bluuebunny/crossref_metadata_2025 huggingface dataset:
Sobre el discurso de seguridad que subyace las prácticas de vigilancia
Authors: Marx Eline
Published: July 2023

El ensayo propone exponer y desmantelar las premisas ontológicas y políticas que dan pie a las prácticas de vigilancia contemporáneas. Argumenta que las políticas y las tecnologías de vigilancia no son abusos sino la continuación de la teoría y práctica política occidental siempre pensada como un proyecto de seguridad estableciendo clases sociales para proteger y otras de las que protegerse. Mientras pretenden proteger nuestras vidas de la inseguridad en las calles, del terrorismo o de un virus, las prácticas de vigilancia maquinan en contra de nuestra autonomía individual y colectiva, definen normas de comportamientos lícitos e ilícitos, criminalizan a grupos sociales precarios, dificultan la identificación de las redes terroristas, crean un mercado en expansión para las empresas de evaluación de riesgos y los estados nacionales, debilitan las reivindicaciones de justicia social y colonizan la esfera pública con un lenguaje bélico. El ensayo denuncia que el proyecto de seguridad liderado en conjunto por actores públicos y privados se encarga de asegurar un negocio lucrativo para los poderosos y un orden social explotador para los desposeídos. Sugiere algunos caminos para oponer el discurso en su fundamento.
Regímenes de propiedad posibles para los datos digitales
Authors: Fraioli Gianni
Published: July 2023

Este trabajo se propone desarrollar la discusión alrededor de la gestión de los datos digitales generados gratuitamente por los usuarios. Para lograrlo trataremos de postular tres regímenes posibles de propiedad de los datos digitales: uno de carácter privado, basado en la implementación de un mercado de datos; otro público, donde hay una centralización de la gestión en manos estatales; finalmente, uno comunitario, donde el control de los datos cae bajo la supervisión de comunidades políticas más pequeñas. Los tres van a funcionar como «tipos ideales» (Weber, 1985), útiles para sintetizar planteos similares actualmentedesperdigados por falta de un corpus teórico lo suficientemente desarrollado. Metodológicamente, estataxonomía tripartita permite señalar alcances y límites de algunas propuestas de distintos autores, a la vez que va a constituir la antesala para la presentación de una alternativa que consideramos superadora en la conclusión.
La semejanza y la mácula
Authors: Valdivia Baselli Alberto
Published: July 2023

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Mindfulness y ansiedad en alumnos de secundaria de una institución educativa privada de Lima
Authors:   , Grunfeld Romero Rebeca Mariella
Published: July 2023

El presente estudio tuvo como propósito hallar la relación entre mindfulness y ansiedad tanto rasgo como estado en alumnos de secundaria de una Institución Educativa Privada de Lima Metropolitana. Se trató de una investigación correlacional, con un diseño no experimental y de corte transversal. La muestra se obtuvo de manera no probabilística e intencional por la investigadora y estuvo conformada por 61 alumnos del primero al quinto año de secundaria cuyas edades fluctuaron entre los 12 y los 17 años. Se utilizó como instrumentos de medición el Five Facet Mindfulness Questionnaire (Baer et al., 2006) y el Inventario de Ansiedad Rasgo-Estado (Spielberger et al., 1975). Los resultados obtenidos evidenciaron que existe una relación estadísticamente significativa e inversa entre el mindfulness y la ansiedad tanto rasgo como estado en la muestra estudiada. Asimismo, se encontró que todas las dimensiones del mindfulness a excepción de la faceta Observación obtuvieron correlaciones significativas, inversas y moderadas con la ansiedad rasgo y que las dimensiones Actuar con Consciencia y Ausencia de Juicio fueron las que mostraron mayor relación con la ansiedad evidenciando valores menores a sig=0.01. Se discutirán las implicaciones de los resultados hallados.
Plan de marketing digital para la empresa Jhanddi
Authors:   , Vásquez Anchelía Eddie Mitchell , Mango Quiroz Daniela Estefani Yessenia , Atiz Hinostroza Edwin Pablo , Palomino Bocanegra Franco Piero
Published: July 2023

La tecnología y las redes sociales están cambiando la forma de trabajar en las empresas y que se están enfocando en la interacción, comunicación directa, seguimiento continuo, estrategias de contenido, etc. En este documento de investigación para la agencia de Viaje “Jhanddi” ubica en Chao, distrito de la Provincia de Virú del departamento de La Libertad, donde se quiere implementar un plan de marketing digital para dar inicio a ser conocida y reconocida como una agencia que brinda calidad de viajes terrestres, precio adecuado y seguridad. Concentrándose en el objetivo primordial en esta investigación es desarrollar el marketing digital, porque la empresa “Jhanddi” tiene 2 años en el mercado ejerciendo su trabajo de manera tradicional, es por ello que en este documento innovaremos la manera de trabajar de la empresa con la ayuda de la tecnología y las redes sociales que es una herramienta muy útil en estos tiempos. También veremos las tablas de inversión, flujo de caja que neta especificando por los 5 primeros años, rentabilidad económica de manera positiva para recuperar la inversión y lo más importante que el proyecto sea viable a largo plazo.
Design and development of 2 x 2 RF MIMO LNA with improved performance characteristics
Authors: Hajamohideen Masood M H , H Umma Habiba , Kunnath Rajesh
Published: July 2023

In this paper, design and development of a 2x2 MIMO RF front end is demonstrated. It has an RF board with commercial transceiver chips and operates at 2.4GHz. In the RF front end the transmitting and receiving paths are separated by using switches. These switches are controlled by transmitter and receiver switches. The signals are then recombined after amplification on both sides. Many advances in radio design and architecture are now allowing for rapid changes in the field of radio design. These changes allow reduction of size, cost, complexity and improve manufacturing by using digital components to replace unreliable and in-accurate analog components. For this to happen, many advances in semiconductor design and fabrication were required and have come to fruition over the last few years. Some of these advances include better integrated mixers, LNA, improved SAW filters, lower cost high performance ADCs and programmable digital tuners and filters.
Meta-Learning based Time-varying Channel Estimation with ICI Cancellation in High-speed Mobile OFDM Systems
Authors: Yang Lihua , Ren Lulu , Shao Yongqi , Chang Ao
Published: July 2023

A meta-learning (ML) based time-varying channel estimation method with inter-carrier interference (ICI) cancellation is proposed for the high-speed mobile OFDM systems. To reduce the effect of the ICI caused by the Doppler shift on the accuracy of channel estimation, a improved transformation matrix is given to transform the transmitted signal with the comb-type pilot in the frequency domain into the one with the block-type pilot in the time domain, which can reduce the interference of the neighboring data symbols to the pilots. Since the ML network has ability to quickly adapt to the new channel scenario, it is employed to estimate the time-varying channel only by the received ICI-free pilots. To improve the practicability of the estimation model, the training target of the network is set as the channel estimation with high accuracy rather than the ideal channel state information. Simulation results show that the proposed method has high estimation accuracy and low computational complexity, and it is robust to the fast time-varying channel in the high-speed mobile scenarios.
Tenki Weather Prediction Model
Authors: CT Manimegalai , Muthu Kali
Published: July 2023

The activities of most sectors rely upon the climate. The environment is changing at an exceptional rate these days, which makes the old forecast strategies less powerful. To foresee the climate, a model is proposed, which combines data preparing, sensors, Google Dataset and machine learning to provide real-time weather monitoring and accomplish climate figures through prescient models valuable for individuals to know the climate conditions constantly.
Path tracking optimization of tractor-trailer wheeled platforms taken into account the wheels dynamics
Authors: Nazemizadeh mostafa , kolahi pouya mallahi , gandomkar mohamadreza
Published: July 2023

Tractor-trailer wheeled platforms are recognized as articulated robotic platforms contain a frontrunner active mobile platform (the tractor) pulling one or more passive ones (the trailers). These categories of mobile platforms are broadly implemented in cooperative activities thanks to their novel features like as: maximum load carrying capacity and minimum energy consumption. The presented article deals with path tracking optimization of the tractor-trailer moving platform employing indirect method of the optimal control. Firstly, the nonlinear motion relations of the modular platform are obtained pertaining to kinematic constraints and inertia characteristics of wheels. Then, the obtained dynamic equations are supposed as constraints of the problem and an appropriate objective criterion is determined for the optimal trajectory tracking. Then, the problem is solved and various simulations are presented. The obtained results reveal proficiency and applicability of the proposed procedure for the optimal tracking of the tractor-trailer wheeled mobile platforms.
The analysis of HPV integration sites based on nanopore sequencing and the profiling changes along the course of photodynamic therapy
Authors: Li Xiulan , Wei Xiaoke , Liu Xin , Wang Nan , Xu Fuqiang , Liu Xingyu , Li Yanmei , Zhou Yuxiang , Tang Huadong , Bian Meina , Hou Ying , Zhang Lili , Wang Weiwei , Liu Qing
Published: July 2023

OBJECTIVE
To detect the HPV genotype and integration sites in patients with high-risk HPV infection at different stages of photodynamic therapy using nanopore technology and to evaluate the treatment effect.
METHODS
Four patients with HPV infection were selected and subjected to photodynamic therapy, and cervical exfoliated cell was sampled at before treatment, after three courses of treatment and six courses of treatment, their viral abundance and insertion sites were analyzed by nanopore technology, and pathological examinations were performed before and after treatment. In this study, we developed a novel assay that combined viral sequence enrichment and Nanopore sequencing for identification of HPV genotype and integration sites at once. The assay has obvious advantages over qPCR or NGS-based methods, as it has better sensitivity after via sequences enrichment and can generate long-reads (kb to Mb) for better detection rate of structure variations, moreover, fast turn-around time for real-time viral sequencing and analysis.
RESULTS
The pathological grade was reduced in all four patients after photodynamic therapy. Virus has been cleared in two cases after treatment, the virus amount reduced after treatment but not completely cleared in one case, and two type viruses were cleared and one type virus persisted after treatment in the last patient with multiple infection. Viral abundance and the number of integration sites were positively correlated. Gene enrichment analysis showed complete viral clearance in 1 patient and 3 patients required follow-up.
CONCLUSION
Nanopore sequencing can effectively monitor the abundance of HPV viruses and integration sites to show the presence status of viruses, and combined with the results of gene enrichment analysis, the treatment effect can be dynamically assessed.
Optimal application of theory in control of unmanned air-craft system - Quadrotor
Authors: Pham Duc Anh , Han Seung Hun
Published: July 2023

Unmanned aerial vehicles (UAVs), particularly Drone-Quadrotors, have gained immense popularity and attracted significant attention. Stability control development and research have emerged as prominent areas of interest among researchers. This study focuses on the design of a controller for a series of quadrotor UAVs with the goal of achieving optimal control while ensuring stability and reaching the desired setpoint. Additionally, our investigation reveals that for miniature UAVs, the internal force acting on the quadrotor is comparatively negligible when compared to the force generated by the control signal. As a result, the non-holonomic nature of the system can be disregarded in the kinetic model of the miniature UAV, while still maintaining satisfactory kinetic performance with the designed controller. The research findings are simulated, compared, and presented using Matlab/Simulink, demonstrating the high efficiency and reliability of the proposed controller.
Hidden impacts of climate change on biological responses of marine life
Authors: Alter Katharina , Jacquemont Juliette , Claudet Joachim , Lattuca María E. , Barrantes María E. , Marras Stefano , Manríquez Patricio , González Claudio P. , Fernández Daniel A. , Peck Myron A. , Cattano Carlo , Milazzo Marco , Mark Felix , Domenici Paolo
Published: July 2023

Conflicting results remain on how climate change affects the biological performance of different marine taxa, hindering our capacity to predict the future state of marine ecosystems. Using a novel meta-analytical approach, we tested for directional changes and deviations across biological responses of fish and invertebrates from exposure to warming (OW), acidification (OA), and their combination. In addition to the established effects of climate change on calcification, survival and metabolism, we found deviations in the physiology, reproduction, behavior, and development of fish and invertebrates, resulting in a doubling of responses significantly affected when compared to directional changes. Widespread deviations of responses were detected even under moderate (IPCC RCP6-level) OW and OA for 2100, while directional changes were mostly limited to more severe (RCP 8.5) exposures. Because such deviations may result in ecological shifts impacting ecosystem structure and processes, our results suggest that OW and OA will likely have stronger impacts than those previously predicted based on directional changes alone.
The Relationship Between the Duration and the Retraction and Atrophy Levels in Traumatic Isolated Full-Thickness Supraspinatus Tears in Young Patients
Authors: ILYAS Gokhan , IPCI Fikri Burak , GOKALP Oguzhan , EGELI Ercument
Published: July 2023

Background
The study aimed to determine the level of retraction and atrophy according to the time elapsed in traumatic isolated full-thickness supraspinatus (SS) tears in young patients.
Methods
One thousand twenty-six patients, who underwent arthroscopic shoulder surgery, were retrospectively reviewed. Pre-operative magnetic resonance imaging (MRI) of 69 patients aged 18 to 40 years with isolated traumatic full-thickness SS lesions remaining after exclusion criteria were evaluated for tendon retraction and atrophy levels. SS retraction was determined from a T2-weighted oblique coronal MRI section, and the atrophy level was determined from the T1-weighted oblique sagittal MRI section. The patients were divided into four groups 0–1 month, 1–3 months, 3–6 months, and 6–12 months according to the time between trauma and MRI. The relationship of tendon retraction and muscle atrophy with elapsed time was evaluated, in addition, comparisons between groups were made.
Results
Thirty-one (45%) of the patients were female and their mean age was 30 ± 7.3 (18–40) years. The mean age of men was 30.5 ± 6.9 (18–39) years (p = 0.880). The time between rupture and MRI was moderately correlated with retraction and strongly correlated with atrophy levels (r = 0.599, .751, respectively). It was observed that there was a statistically significant difference between the 1st (0–1 month) and 2nd (1–3 months) groups (p = 0.003, .001, respectively), and between the 2nd and 3rd (3–6 months) groups (p = 0.032, .002, respectively), but there was no significant difference between the 3rd and 4th (6–12 months) groups (p = 0.118, .057, respectively). In addition, there was a moderate correlation between tendon retraction and atrophy levels (r = 0.668). Power (1- b) in post hoc analysis was calculated as 0.826.
Conclusions
In traumatic full-thickness SS tears, the moderate positive correlation between the time elapsed after trauma with the level of retraction, and a strong correlation with the level of atrophy showed the importance of early surgical repair in young patients.
Effect and prediction of molecular subtype, metastatic site and treatment for early death in non-small cell lung cancer: A SEER-based study
Authors: cao zhuo , yao rui , hu jiapei , Hou Xiaomin , Liu Hang , li ke
Published: July 2023

Background:
early death in patients with non-small cell lung cancer remains an unresolved issue. Therefore, it is necessary to identify the risk factors for early death in non-small cell lung cancer patients.
Purpose:
The purpose of this study was to identify associated risk factors and develop a predictive nomogram for the early death of non-small cell lung cancer patients.
Research method:
We enrolled 51529 patients in SEER Database who were 60 years or older, diagnosed with primary non-small cell lung cancer, from 2010 to 2016. All patients were randomly divided into training and testing sets at 7:3. Multivariate COX proportional hazard regression was used to identify independent risk variables for early death. Use K-M survival curve to compare different molecular subtypes, metastatic sites, and treatment methods on the survival status of lung cancer patients. A predictive nomogram was developed to predict the 1-year overall survival (OS) and then validated by calibration curves, receiver operating characteristic (ROC) curves, and decision curve analysis (DCA).
RESULTS:
Eight independent variables, including race, sex, age, tumor size, AJCC M, molecular subtype, metastatic site and treatment were recognized by using multivariate COX proportional hazard regression model for identifying independent risk variables of early death about non-small cell lung cancer patients. By comprising these variables, a predictive nomogram was constructed in the training set cohort. In the nomogram of 1-year overall survival, the areas under the ROC curves were 0.781 (95% CI: 0.771–0.804) and for the training dataset as well as (95% CI: 0.761–0.821) for the validation dataset. Then, the consistency between the predicted and actual overall survival was confirmed by the calibration curves. Additionally, the results of the DCA indicated that the predictive model had a favorable clinical applicability.
Conclusion:
In the early stages of non-small cell lung cancer (NSCLC), different molecular subtypes, metastatic sites, and treatment methods can significantly affect the survival rate of patients. The nomogram model developed in this study provides an insightful and applicable tool for predicting the risk of early mortality in NSCLC patients. It can help clinicians identify patients at high risk of early death and tailor their treatment plans accordingly, potentially improving patient outcomes.
Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells
Authors: Méndez Rui Santalla , Furones Andrea Rodgers , Classens René , Fedorova Kristina , Haverdil Manon , Capdevila Marta Canela , Duffelen Anne van , Spruijt Cornelia G , Vermeulen Michiel , Beest Martin ter , Spriel Annemiek van , Cano Laia Querol
Published: July 2023

Intracellular vesicle transport is essential for cellular homeostasis and is partially mediated by SNARE proteins. Endosomal trafficking to the plasma membrane ensures cytokine secretion in dendritic cells (DCs) and the initiation of immune responses. Despite its critical importance, the specific molecular agents that regulate DC cytokine secretion are poorly characterised. Galectin-9, a ß-galactoside-binding protein, has emerged as a novel cellular modulator although its exact intracellular roles in regulating (immune) cell homeostasis and vesicle transport are virtually unknown. We investigated galectin-9 function in primary human DCs and report that galectin-9 is essential for intracellular cytokine trafficking to the cell surface. Galectin-9-depleted DCs accumulate cytokine-containing vesicles in the Golgi complex that eventually undergo lysosomal degradation. We observed galectin-9 to molecularly interact with Vamp-3 using immunoprecipitation-mass-spectrometry and identified galectin-9 was required for rerouting Vamp-3-containing endosomes upon DC activation as the underlying mechanism. Overall, this study identifies galectin-9 as a necessary mechanistic component for intracellular trafficking. This may impact our general understanding of vesicle transport and shed new light into the multiple roles galectins play in governing cell function.
Weathering of accretionary orogens as a trigger for the Cambrian Explosion
Authors: Zhu Mingshuai , Zhang Yinggang , Pastor-Galán Daniel , Smit Matthijs , Mills Benjamin J. W. , Zhang Fuqin , Guilmette Carl , Miao Laicheng , Li Shun , Ganbat Ariuntsetseg , Yang Shunhu
Published: July 2023

The Cambrian Explosion (540-515 Ma ago) is arguably the most significant evolutionary transition after the origin of life. A variety of environmental perturbations including rising oxygen levels, changes in ocean chemistry and increased bio-essential elements have been correlated to this rapid faunal diversification. Anomalously high weathering fluxes from the continents to the oceans are hypothesized to cause these perturbations, but there is no obvious candidate to produce such enhanced weathering events. The Gondwana’s peripheral orogens and Central Asian Orogenic Belt, provide a unique and unrecognized global accretionary orogen (>18,500 km) concomitant to the Cambrian Explosion, larger than the so-far recognized Cambrian collisional orogens. Our modeling results suggest that the increased weathering of phosphorus from these Cambrian accretionary orogenic belts would stimulate marine primary productivity, increase atmospheric O2, and promote the expansion of shallow-ocean oxygenation at a time coincident with the Cambrian Explosion.
High GLUT1 membrane expression and low PSMA membrane expression in Ductal Adenocarcinoma and Intraductal Carcinoma of the prostate
Authors: Cai Yi , Wang Xingming , Zhou Li , Qi Lin , Zhang Ye , Yin Hong-ling , Gan Yu , Gao Xiaomei
Published: July 2023

BACKGROUND
Both Ductal Adenocarcinoma (DAC) and Intraductal Carcinoma (IDC) of the prostate are generally associated with aggressive clinical behavior and poor prognosis, which were linked with discordant FDG positivity and low Prostate-Specific Membrane Antigen (PSMA) expression. A recent study only cited a DAC patient with low 68Ga-PSMA-11 PET/CT uptake but high 18F-FDG PET/CT uptake, however, there is lack of directly compared articles nor large data sets. Hence, the objective of this study was to investigate the expression of PSMA and GLUT1 in DAC and IDC-P patients.
METHODS
The study was conducted on 87 DAC or/and IDC-P patients without any treatment and 97 PAC patients with a Gleason score ≥ 8 of prostate biopsies and prostatectomy samples between August 2017 and August 2022. We performed immunohistochemical staining and scoring of various cancer component samples from the patients to reflect the protein expression levels of PSMA and GLUT1.
RESULTS
PSMA expression in PAC was significantly higher than in DAC/IDC-P (141.2 vs 78.6, p < 0.001). There was no significant difference in PSMA expression between DAC/IDC-P and adjacent PAC (78.6 vs 93.4, p = 0.166). GLUT1 expression was higher in DAC/IDC-P than in adjacent PAC (68.6 vs 51.3, p = 0.007), but was still lower than that in pure PAC (68.6 vs 93.1, p = 0.0014). It is worth noting that GLUT1 membrane expression in DAC/IDC-P was significantly increased than in pure PAC (13.0 vs 6.6, p = 0.025), and in PAC adjacent to DAC/IDC-P (13.0 vs 2.0, p < 0.001).
CONCLUSIONS
In DAC/IDC-P tissues, PSMA expression is low, while GLUT1 expression, especially GLUT1 membrane expression is high. These findings imply that DAC/IDC-P may have higher glucose metabolic and raise interest in targeting membrane GLUT1 as a novel anticancer strategy for DAC/IDC-P and other prostate cancer with high glucose metabolism.
Anti-Candida sp Activity of Astronium urundeuva derivatives Free and Loaded into a Nanostructured Lipid System
Authors: Bonifácio Bruna Vidal , Carvalho Flávio Alexandre , Mota Luiza de Oliveira , Silva Patrícia Bento da , de Souza Leonardo Perez , Vilegas Wagner , Chorilli Marlus , Santos André Gonzaga dos , Bauab Taís Maria
Published: July 2023

Infections caused by Candida strains increased, as well as strains resistant to the most conventional antimicrobials. Thus, the search for new natural sources of antimicrobials without adverse effects also increased. In this case, Astronium urundeuva Engl., a medicinal plant with several pharmacological activities, including antifungal activity, emerges as a source of antifungal compounds. Here, we evaluated the antifungal activity of A. urundeuvaand its derivatives free or loaded into a nanostructured lipid system, and combination studies against Candidastrains. A. urundeuva components purification and identification were performed through chromatographic and spectroscopic techniques, respectively. The major components identified were gallic acid (1), methyl gallate (2), ethyl gallate (3), and 1,2,3,4,6-penta-O-galoyl-β-D-glucose (PGG) (4). A. urundeuva extract, its aqueous fraction, and PGG (4) exhibited the same MIC values between 0.2 - 62.5 µg/mL against Candida strains being classified as significant activity, while the amphotericin B MIC was 0.1 - 1.2 µg/mL. Combination assay demonstrated that may occur synergism (0.37) between gallic acid (1) x methyl gallate (2). Thus, the results demonstrated as PGG (4) much as the gallic acid (1) x methyl gallate (2) combined might be the main responsible for the antifungal activity against Candida strains, mainly C. glabrata ATCC 2001 and that the tested nanostructured system maintained the antifungal activity. Through the antifungal activity of the extract and purified compounds, the extract may be employed in the development of antifungal herbal medicine, because its purification process is simple and greener than pure compounds.
Advancements in the Research of Modified Hydroxyapatite Materials for Bone Repair : a Scoping Review
Authors: Huang Leiyun , Bai Zhenglin , Fu Guangjie , Meng Zengdong
Published: July 2023

Modified hydroxyapatite (mHA) materials have shown efficacy in bone defect repair, exhibiting excellent properties such as biocompatibility, mechanical performance, antibacterial activity, and degradability. This article aims to consolidate existing research on the various aspects of mHA materials, discussing their superior performance and the fundamental principles of osteoinduction. Additionally, it explores the potential of these materials to facilitate the exploration of novel bone repair materials in the future. The primary objective of this study is to provide new insights into optimizing the functionality and structure of bone repair materials, as well as enhancing their biocompatibility, mechanical properties, antibacterial capabilities, and degradability. A systematic search was conducted in PubMed and Web of Science databases for relevant articles on modified hydroxyapatite materials. A total of 601 articles were retrieved, 461 articles with poor correlation with the purpose of the study, obsolete content, repetition and stage of the same author were excluded, and 140 articles that met the criteria were reviewed. The critical analysis and synthesis were based on the examination of in vitro and in vivo experimental results, and theoretical foundations of mHA materials. The discussion section addresses the current challenges and identifies future directions in this field.
Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2
Authors: Li Le , Feng Tang , Liu Jiyan , Si Longlong
Published: July 2023

The host immunity induced by wide spread of Omicron variants and its influence on emerging SARS-CoV-2 variants are attracting broad attention. Here we report that the natural infection with Omicron BA.5.2 induce broad cross-reactive immunity against SARS-CoV-2 variants, which could reduce the risk of next wave of SARS-CoV-2 infection.
Enhancing Properties of Thermoplastic Starch/Natural Rubber Blends through the Synergistic Combination of PEG and Modified Natural Rubber
Authors: Songtipya Ladawan , Kalkornsurapranee Ekwipoo , Songtipya Ponusa , Sengsuk Theerarat , Promsung Rawiporn , Chuaybamrung Arthittaya , Johns Jobish
Published: July 2023

This study focuses on enhancing the mechanical and thermal properties of thermoplastic starch (TPS) and natural rubber (NR) blends through the incorporation of polyethylene glycol (PEG 2,000) and various types of modified natural rubber such as epoxidized natural rubber (ENR), poly(methyl methacrylate) grafted natural rubber (NR-g-PMMA), and poly(butyl methacrylate) grafted natural rubber (NR-g-PBMA). The influence of TPS/NR blend ratios, PEG content, and type of modified NR on the properties of the blends was investigated along with their water absorption and bio-degradation. The increase in ductile properties of the TPS/NR blends can be achieved by increasing the NR content. Among the series of TPS/NR blends, the highest toughness (1,630 MJ/m3) can be observed when the blend was formulated from 1.0 wt% of PEG and 10.0 wt% of ENR. The water absorption of TPS/NR blends was found to be lower than the TPS sample, but still exhibited a high water absorption rate compared to the other conventional polymers. The bio-degradation test confirmed the bio-degradation capability of TPS/NR blends according to ASTM 5988-12, and the result revealed that more than 80% of the tested samples can be bio-degraded in soil within 90 days.
Optimization of a new EAE induction strategy by subcutaneous administration of a Eukaryotic RpfE- MOG(35-55) Expressing Plasmid
Authors: Ziaei Maryam , Aliomrani Mehdi , Javdani Moosa , Shakhsi-Niaei Mostafa
Published: July 2023

Multiple Sclerosis (MS) is considered as the common autoimmune inflammatory disease; while, Experimental Autoimmune Encephalomyelitis (EAE) is the most recognized common animal model of the human MS disease. The present study aimed at introducing a new and simple method for induction of chronic EAE in C57BL/6J mice by using a eukaryotic vector. In this method, Resuscitation-promoting factor-E (RpfE) as an adjuvant, and Myeline oligodendrocyte glycoprotein (MOG(35−55)), as neuro-antigen were cloned into pcDNA3.1(+) IRES GFP. Thereafter, the mice received two doses of plasmids in the 1st and 7th days of experiment as follows: group A, 16–16 µg; group B, 32 − 16 µg; group C, 32–32 µg; group D, 64–64 µg and group E was the control group. The clinical scores during 9 weeks were documented before they were euthanized. There were not only significant differences in the clinical symptoms initiation and severity amongst groups but also in the time of showing highest inflammatory responses, which was about two weeks after clinical symptoms onset. Histopathological results revealed different degrees of demyelination as well as CD4+, CD8+, and CD20+ cell infiltration into the CNS tissues. High doses of the recombinant plasmid (64–64 µg) led to higher CD4+ cell accumulation, lesser demyelination, and delayed EAE induction (week 5); while, lower doses (up to 32–32 µg) resulted in higher CD8+ cells penetration, greater demyelination, and earlier EAE initiation (week four). To sum up, different doses of this eukaryotic plasmid is suggested as a simpler and about 3-times cheaper technique for induction of different pathological features of EAE model in mice.
Programmable Mixed-Signal Circuits
Authors: Tappertzhofen Stefan
Published: July 2023

A novel concept for programmable mixed-signal circuits is presented based on programmable transmission gates. For implementation, memristively switching devices are suggested as the most promising candidates for realization of fast and small-footprint signal routing switches with small resistance and capacity. As a proof-of-concept, LT Spice simulations of digital and analogue example circuits implemented by the new concept are demonstrated. It is discussed how important design parameters can be tuned in the circuity. Compared to competing technologies such as Field Programmable Analogue Arrays or Application-Specific Integrated Circuits, the presented concept allows for development of ultra-flexible, reconfigurable, and cheap embedded mixed-signal circuits for applications where only limited space is available or high bandwidth is required.
Spatio-temporal Simulation and Statistical Analysis of Soil Total Nitrogen in Alpine Meadow of Gannan
Authors: 张 锐祺 , Zhang Meiling , Jia Xiaonan
Published: July 2023

To determine the temporal and spatial characteristics of soil nitrogen in the alpine meadow of Gannan, China, the contents of soil organic nitrogen, mineral nitrogen and soil total nitrogen in Gannan alpine meadow from 1971 to 2020 were simulated based on the localized CENTURY model, on this basis, the spatial and temporal distribution of soil nitrogen of the study area was visualized. The influence of various environmental factors on soil nitrogen in the alpine meadow of Gannan was analyzed. The results show that from 1971 to 2020, the soil organic nitrogen in the study area has moderate spatial variability. Active soil organic nitrogen pool and chronic soil organic nitrogen pool accounted for 11.2% and 39.8% of total soil organic nitrogen, respectively. The inert soil organic nitrogen pool accounts for 49.0% of the total organic nitrogen in the soil. In spatial distribution, the total organic nitrogen and total mineral nitrogen in the alpine meadow of Gannan showed a decreasing trend from west to east, and the total mineral nitrogen content in the soil decreased significantly with the increase of soil depth. Correlation analysis of soil nitrogen and environmental factors and structural equation model showed that precipitation, elevation, soil organic matter content and silt content had positive correlation with soil organic nitrogen and mineral nitrogen contents. The effects of sand content and temperature on soil organic nitrogen and mineral nitrogen contents were negatively correlated.
Gas cavities in the chest wall, a report of Clostridium perfringens infection
Authors: Li Kun , Huang Xiaohan , Gu Silan , Cai Hongliu , Huang Lingtong
Published: July 2023

We reported a case of Clostridium perfringens infection with Gas activities in the Chest wall. Unlike the widely reported of gas cavity in liver, gas cavities in the chest wall caused by Clostridium perfringens infection is rare. Extrahepatic gas cavities indicate bloodstream infection and tissue invasion of Clostridium perfringens which can be an indicator of poor disease outcome.
Effects of multiple cations and sintering temperature on microstructure and dielectric properties in Na1/2Ln1/2Cu3Ti4O12 (Ln = Sm and Eu) ceramic materials
Authors: Yuan Long-Fei , Zhang Ting , Han Dan-Dan
Published: July 2023

Na1/2Eu1/2Cu3Ti4O12 and Na1/2Sm1/2Cu3Ti4O12 dielectric ceramics were synthesized at different sintering temperatures (950, 975 and 1000 oC) by a solid-state reaction method. Phase structure, cation valence state, and dielectric properties of all sintered ceramics were systematically investigated. When the preparation temperature was changed, the Cu+ ion concentration of (Na+, Eu3+) co-doped ceramics changed faster than that of (Na+, Sm3+) co-doped ceramics. Abnormally high dielectric permittivity ~3.17 Í 104 were accomplished of Na1/2Sm1/2Cu3Ti4O12 ceramic at a sintering temperature 950 oC. However, Na1/2Eu1/2Cu3Ti4O12 and Na1/2Sm1/2Cu3Ti4O12 prepared in high sintering temperature (1000 oC) exhibited a good dielectric stability and low loss tangent (0.061 and 0.102 at 10 Hz, respectively). It was demonstrated that an increasing number of charge carriers induced by the increase of sintering temperature could lead to a competitive coexistence of two polarization mechanisms (surface barrier layer capacitor and internal barrier layer capacitor), further changing the dielectric properties of CaCu3Ti4O12-based ceramics.
Bushen Tiaochong Recipe induces resistance to apoptosis in oocytes by activating the JNK pathway, inhibiting mitochondrial autophagy and promoting mitochondrial fusion
Authors: Li Shuang , Zhang Yin-Feng , Han Ying , Wang Xin-Yan , Zhang Yun-Shan , Luo Hai-Ning
Published: July 2023

Background: The mechanisms underlying ovarian function regulation and decline have been a hot topic of research. Based on the traditional Chinese medicine concept of "pattern differentiation-physiologic system-pathway analysis," a systematic characterization method can be used to investigate these issues. In this study, we used microproteomics techniques to compare the differential protein expression in oocytes of diminished ovarian reserve (DOR) mice, and drug-treated mice, and to comprehensively analyze the regulatory effects of Bushen Tiaochong Recipe (BSTCR) on the level and functional effects of protein expression in DOR mice.
Methods: There were 10 mice in each of the groups, which included the blank group, the model group, the Western medicine group, and the traditional Chinese medicine group. After 6 weeks of gavage, superovulation was induced in the mice, and seven eggs was collected from each mouse for testing. We analyzed and compared the differentially expressed proteins in oocytes of DOR mice and drug-treated mice.
Results: Using microproteomic technology, we found that the expression of the apoptosis-related proteins BAX and MK09 was significantly downregulated after treatment with BSTCR, and this may inhibit oocyte apoptosis by affecting the JNK-c-Jun pathway. The core proteins UBP30 and MOB1B were significantly downregulated, inhibiting mitophagy to exert antiapoptotic and cell growth effects, which are potential targets of BSTCR.
Conclusion: Overall, we found that BSTCR regulates the expression of MFN1, promotes mitochondrial fusion, and enhances mitochondrial function. Furthermore, BSTCR exerts anti-apoptotic and growth-promoting effects on oocytes by inhibiting mitochondrial autophagy and promoting mitochondrial fusion.
Myxobolus longirostris sp. n. (Myxozoa: Myxobolidae) infecting the brain of Chinese longsnout catfish Leiocassis longirostris (Günther, 1864) in China
Authors: Zhao Xiaojing , Xu Liwen , Wei Qiang , Yin Qi , Yang Yijing , Zhang Jinyong , Xiang Jianguo , Yu Jianbo , Li Deliang , Liu Xinhua
Published: July 2023

During an investigation of Myxobolus diversity in the Chinese longsnout catfish Leiocassis longirostris (Günther, 1864), a new species infecting the intracranial epidermis of the host was discovered. Upon opening the cranial cavity, several round whitish plasmodia measuring 0.55–0.80 mm in diameter were observed. Fresh spores (n = 50) were pyriform in the frontal view and fusiform in the sutural view, with a length of 15.4 ± 0.6 (13.9–16.5) µm, width of 9.1 ± 0.4 (8.3–9.8) µm, and thickness of 7.0 ± 0.4 (6.3–7.9) µm. The spores had smooth shell surfaces and transparent membrane sheaths in the posterior. No folds, intercapsular appendix, and caudal appendages were observed. Two equal polar capsules were pyriform and measured 7.5 ± 0.5 (6.7–8.7) µm in length and 3.2 ± 0.3 (2.5–3.6) µm in width. The polar filaments were coiled with five to six turns and perpendicular to polar capsule length. A BLAST search indicated Myxobolus longirostris sp. n. was closely related to four Myxobolus species (with sequences similarities ranging from 91.8–97.1%) found in different organs of yellow catfish Tachysurus fulvidraco, rather than the Leiocassis-infecting species M. branchiola (with 90.5% sequence similarity). Phylogenetic analysis showed that Myxobolus longirostris sp. n. clustered with M. jianlinensis and M. voremkhai within the Siluriformes-clade with highly supported values. This suggests that the host may play an important role in the evolution of Myxobolus species. Based on the morphological, ecological, and molecular differences observed between the newly discovered species and other available Myxobolus species, Myxobolus longirostris sp. n., is proposed and described in this study.
Influence of Samarium Doping on Structural, thermoelectrical properties of Nano Crystalline Cobalt Ferrite
Authors: Siddeshwar M. , Boda Nehru , Chittury Kanchana Latha , S B Shanti
Published: July 2023

Samarium substituted Cobalt ferrite nanoparticles with chemical formula CoSmXFe2-XO4 (where X=0.0, 0.010, 0.030, 0.050, 0.070 0.090 and 0.10) were synthesized via citrate-gel auto combustion method. Followed by annealing at 500 °C for 4 h in air. The physical properties of the sample were detected through x-ray diffraction, Fourier transformation infrared spectroscopy transmission electron microscopy, scanning electron microscopy, thermo electric power measurements, and dielectric measurement techniques. X-ray diffraction (XRD) analysis showed single-phase cubic spinel structure of the ferrites and the values of lattice constant (a) and x-ray density (dx) increased with the increase of Sm3+ content. The surface morphology of the samples was observed by scanning electron microscopy (SEM). Thermoelectric power studies of prepared samples were carried out from room temperature to well beyond Transition temperature (K) with a differential method. The seebeck coefficient variation with temperature was studied and it shows the prepared samples are n-type semiconducting nature. The transition temperatures of the prepared samples were also observed from thermoelectric power studies measurements. The dielectric parameters like real part of dielectric constant(εʹ), imaginary part dielectric constant (εʺ), Loss tangent(Tanδ), AC Conductivity, of the samples were studied as a function of frequency in the range of 50Hz to5MHz at room temperature using Agilent E4980 were studied. The observed result can be explained on the basis of conduction mechanism.
Impact of care disruption during Covid-19 on the Mental and Physical Well-being of cancer survivors: a nationally representative survey-based study
Authors: Elkefi Safa , Matthews Alicia K.
Published: July 2023

Purpose
Our study explores the care disruption in cancer care among the different demographic subgroups. It also investigates the impact of care disruption on cancer survivors’ mental and physical well-being.
Methods
This study utilized the HINTS-SEER data (Health Information Trends Survey for Surveillance Epidemiology and End Results). Missing data was addressed using the K-nearest-neighbor algorithm. Descriptive statistics were conducted to understand the care disruption perceptions. Partial least squares structural equation models were employed for data analysis.
Results
Most of the cancer survivors thought that Covid-19 impacted their cancer treatment or cancer follow-up medical appointments (69.45%), their routine cancer screening or preventative care (60.70%), and their cancer treatment plans (73.58%). Only 28.03% of them thought the pandemic changed their interactions with their healthcare providers (HCPs). Elderly patients were more likely to report visits and appointment disruption. The interactions with HCPs showed significant correlations with the demographics. People who thought their contact with their doctors changed during Covid-19 are more likely to be older adults, white, or with higher income. Mental and physical well-being were negatively impacted by the changes in cancer treatment and visits but not by changes in the interactions with HCPs.
Conclusions & implications for cancer survivors
These findings underscore the importance of maintaining regular care for cancer survivors. They also emphasize the need for targeted interventions to address disparities in access to cancer treatment and follow-up care among the different demographic groups. Maintaining communication between survivors and HCPs may have played an important role in mitigating care disruption.
SUMOylation in astrocytes induces changes in the proteome of the derived small extracellular vesicles which change protein synthesis and dendrite morphology in target neurons
Authors: Fernandez Anllely , Corvalan Katherine , Santis Octavia , Méndez-Ruette Maxs , Caviedes Ariel , Pizarro Matias , Gomez Maria-Teresa , Batiz Luis Federico , Landgraf Peter , Kahne Thilo , Rojas-Fernandez Alejandro , Wyneken Ursula
Published: July 2023

Emerging evidence highlights the relevance of the protein post-translational modification by SUMO (Small Ubiquitin-like Modifier) in the central nervous system for modulating cognition and plasticity in health and disease. In these processes, astrocyte-to-neuron crosstalk mediated by extracellular vesicles (EVs) plays a yet poorly understood role. Small EVs (sEVs), including microvesicles and exosomes, contain a molecular cargo of lipids, proteins, and nucleic acids that define their biological effect on target cells. Here, we investigated whether SUMOylation globally impacts the sEV protein cargo. For this, sEVs were isolated from primary cultures of astrocytes by ultracentrifugation or by the use of a commercial sEV isolation kit. SUMO levels were regulated: 1) via plasmids that over-express SUMO, or 2) via experimental conditions that increase SUMOylation, i.e., by using the stress hormone corticosterone, or 3) via the SUMOylation inhibitor 2-D08 (2′,3′,4′-trihydroxy-flavone, 2-(2,3,4-Trihydroxyphenyl)-4H-1-Benzopyran-4-one). Corticosterone and 2-D08 had opposing effects on the number of sEVs and on their protein cargo. Proteomic analysis showed that increased SUMOylation in corticosterone-treated or plasmid-transfected astrocytes increased the presence of proteins related to cell division, transcription, and protein translation in the derived sEVs. When sEVs derived from corticosterone-treated astrocytes were transferred to neurons to assess their impact on protein synthesis using the fluorescence non-canonical amino acid tagging assay (FUNCAT), we detected an increase in protein synthesis, while sEVs from 2-D08-treated astrocytes had no effect. Our results show that SUMO conjugation plays an important role in the modulation of the proteome of astrocyte-derived sEVs with a potential functional impact on neurons.
Homoharringtonine enhances cytarabine induced apoptosis in acute myeloid leukemia by regulating p38 MAPK/H2AX/Mcl-1 axis
Authors: Qiu Yang , Bai Lu , Zhao Haosen , Mei Xifan
Published: July 2023

Acute myeloid leukemia (AML) is a fatal disease of hematopoietic malignancies and treated with the conventional combination of cytarabine (Ara-C) and daunorubicin (Dau). Homoharringtonine (HHT) is a natural alkaloid obtained from Cephalotaxus species which was used in China for AML combination treatment. Clinically it has been reported HHT plus Ara-C was equally effective as Dau plus Ara-C in some types of AML patients with less toxic effect. We found HHT downregulates Mcl-1, phosphorylates H2AX and induces apoptosis by activating p38 MAPK. Inactivation of p38 through inhibitors and siRNA blocks apoptosis, H2AX phosphorylation and Mcl-1 reduction. HHT enhances Ara-C activation of p38 MAPK signaling pathway, overcoming Ara-C tolerance to cell apoptosis by regulating the p38/H2AX/Mcl-1 axis. 1:4 (M/M) is the optimal ratio of HHT and Ara-C for AML cell synergistic lethality. HHT synergistically induces apoptosis with Ara-C in vitro and prolongs survival of THP-1 xenografts. We provide a new mechanism for AML treatment by regulating p38 MAPK/H2AX/Mcl-1 axis to improve cytarabine therapy.