bluuebunny/crossref_metadata_2025 huggingface dataset:
Sediment classification in the paleo-oceanic environment based on multi-acoustic reflectance characteristics in the Southern Tianshan Mountains
Authors: Zhen Huancheng , Cao Xinghui , Qu Zhiguo , Zou Dapeng , Xiong Shuai , Song Jiang , Guo Hao
Published: August 2024

The grain size of sediments is a crucial parameter in sedimentology, with significant implications for submarine engineering and water conservancy projects. In this study, we developed an acoustic reflection measurement system using a self-developed, high-precision, high-frequency shallow stratigraphic profiler. The system's accuracy was validated with standard acrylic samples. Results showed that within the sediment grain size range of 0.3 to 2.5 mm, the acoustic reflection amplitude increased with grain size. However, distinguishing grain sizes between 0.1 and 0.3 mm from those between 1.0 and 1.5 mm based solely on reflection amplitude proved challenging. Notably, the differences in wavefront flare shapes between these grain sizes were readily apparent. Therefore, combining reflection peak amplitude with time-domain waveform analysis enables more precise sediment grain size classification.
Production and characterization of biodiesel fuel produced from third-generation feedstock
Authors: Verma Suraj , Sahu Deepak , Almutairi Bader O.
Published: August 2024

Biodiesel is an eco-friendly, renewable alternative fuel, and it can be obtained from soybean oil, vegetable oils, animal fat, or microalgae. This study presents a comprehensive investigation into the production and characterization of microalgae biodiesel utilizing multiple analytical techniques, including CHNSO analysis, Fourier-transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GC–MS), and proton nuclear magnetic resonance spectroscopy (1H NMR). The CHNSO analysis revealed the elemental composition of biodiesel blends, highlighting the effects of TiO2 nanoparticle concentrations on carbon, nitrogen, sulfur, and oxygen content. With increasing TiO2 concentration, a steady increase in the carbon content and a gradual decrease in the nitrogen content were observed. According to the CHNSO analysis, the sulfur content of blended biodiesel was found to be lower than that of fossil diesel, with an empirical formula of CH2.26N0.000584S0.000993O0.0517. FTIR and 1H NMR spectroscopy confirmed the synthesis of biodiesel. Fourier-transform infrared resonance confirmed the presence of ester groups at 1732 cm-1, and a prominent peak at 1,455 cm-1 indicated a higher carbon content in the blended biodiesel. GC–MS analysis identified compounds of fatty acid methyl esters (FAMEs) and hydrocarbons. The major components of FAMEs were 9-octadecenoic acid methyl ester (C19H36O2), linoleic acid ethyl ester (C20H36O2), and hexadecanoic acid methyl ester (C17H34O2), with compositions 20.65%, 9.67%, and 6.26%, respectively. The presence of methyl ester in the blended fuel suggests its potential as an alternative fuel source.
Soft ankle exoskeleton to counteract dropfoot and excessive inversion
Authors: Zhang Xiaochen , Liu Yi-Xing , Wang Ruoli , Gutierrez-Farewik Elena M.
Published: August 2024

Introduction

Wearable exoskeletons are emerging technologies for providing movement assistance and rehabilitation for people with motor disorders. In this study, we focus on the specific gait pathology dropfoot, which is common after a stroke. Dropfoot makes it difficult to achieve foot clearance during swing and heel contact at early stance and often necessitates compensatory movements.

Methods

We developed a soft ankle exoskeleton consisting of actuation and transmission systems to assist two degrees of freedom simultaneously: dorsiflexion and eversion, then performed several proof-of-concept experiments on non-disabled persons. The actuation system consists of two motors worn on a waist belt. The transmission system provides assistive force to the medial and lateral sides of the forefoot via Bowden cables. The coupling design enables variable assistance of dorsiflexion and inversion at the same time, and a force-free controller is proposed to compensate for device resistance. We first evaluated the performance of the exoskeleton in three seated movement tests: assisting dorsiflexion and eversion, controlling plantarflexion, and compensating for device resistance, then during walking tests. In all proof-of-concept experiments, dropfoot tendency was simulated by fastening a weight to the shoe over the lateral forefoot.

Results

In the first two seated tests, errors between the target and the achieved ankle joint angles in two planes were low; errors of <1.5° were achieved in assisting dorsiflexion and/or controlling plantarflexion and of <1.4° in assisting ankle eversion. The force-free controller in test three significantly compensated for the device resistance during ankle joint plantarflexion. In the gait tests, the exoskeleton was able to normalize ankle joint and foot segment kinematics, specifically foot inclination angle and ankle inversion angle at initial contact and ankle angle and clearance height during swing.

Discussion

Our findings support the feasibility of the new ankle exoskeleton design in assisting two degrees of freedom at the ankle simultaneously and show its potential to assist people with dropfoot and excessive inversion.
Cluster headache: understandings of current knowledge and directions for whole process management
Authors: Xu Xiao-Hu , Li Yi-Ming , Ren Li-Na , Xu Xiao-Fan , Dai Yi-Long , Jin Cheng-Qiang , Yang Rui-Rui
Published: August 2024

Cluster headache (CH) is a common primary headache that severely impacts patients’ quality of life, characterized by recurrent, severe, unilateral headaches often centered around the eyes, temples, or forehead. Distinguishing CH from other headache disorders is challenging, and its pathogenesis remains unclear. Notably, patients with CH often experience high levels of depression and suicidal tendencies, necessitating increased clinical attention. This comprehensive assessment combines various reports and the latest scientific literature to evaluate the current state of CH research. It covers epidemiology, population characteristics, predisposing factors, and treatment strategies. Additionally, we provide strategic insights into the holistic management of CH, which involves continuous, individualized care throughout the prevention, treatment, and rehabilitation stages. Recent advances in the field have revealed new insights into the pathophysiology of CH. While these findings are still evolving, they offer a more detailed understanding of the neurobiological mechanisms underlying this disorder. This growing body of knowledge, alongside ongoing research efforts, promises to lead to the development of more targeted and effective treatments in the future.
Promising therapeutic effects of high-frequency repetitive transcranial magnetic stimulation (HF-rTMS) in addressing autism spectrum disorder induced by valproic acid
Authors: Afshari Masoud , Gharibzadeh Shahriar , Pouretemad Hamidreza , Roghani Mehrdad
Published: August 2024

Introduction

Autism spectrum disorder (ASD) is a neurodevelopmental condition that affects various regions of the brain. Repetitive transcranial magnetic stimulation (rTMS) is a safe and non-invasive method utilized for stimulating different brain areas. Our objective is to alleviate ASD symptoms using high-frequency rTMS (HF-rTMS) in a rat model of ASD induced by valproic acid (VPA).

Methods

In this investigation, we applied HF-rTMS for ASD treatment, focusing on the hippocampus. Behavioral assessments encompassed core ASD behaviors, as well as memory and recognition tests, alongside evaluations of anxiety and stress coping strategies. Additionally, we analyzed oxidative stress and a related inflammation marker, as well as other biochemical components. We assessed brain-derived neurotrophic factor (BDNF), Microtubule-associated protein-2 (MAP-2), and synaptophysin (SYN). Finally, we examined dendritic spine density in the CA1 area of the hippocampus.

Results

The results demonstrated that HF-rTMS successfully mitigated ASD symptoms, reducing oxidative stress and improving various biochemical factors, along with an increase in dendritic spine density.

Discussion

Collectively, our data suggests that HF-rTMS may effectively alleviate ASD symptoms. These findings could be valuable in clinical research and contribute to a better understanding of the mechanisms underlying ASD.
Plant but not animal sourced nitrate intake is associated with lower dementia-related mortality in the Australian Diabetes, Obesity, and Lifestyle Study
Authors: Rajendra Anjana , Bondonno Nicola P. , Zhong Liezhou , Radavelli-Bagatini Simone , Murray Kevin , Rainey-Smith Stephanie R. , Gardener Samantha L. , Blekkenhorst Lauren C. , Magliano Dianna J. , Shaw Jonathan E. , Daly Robin M. , Anstey Kaarin J. , Lewis Joshua R. , Hodgson Jonathan M. , Bondonno Catherine P.
Published: August 2024

Introduction

Dietary nitrate is potentially beneficial for cardiovascular, cerebrovascular, and nervous systems due to its role as a nitric oxide (NO) precursor. Increased nitrate intake improves cardiovascular health and therefore could protect against dementia, given the cardiovascular-dementia link.

Objective

To investigate the association between source-dependent nitrate intake and dementia-related mortality. As individuals with diabetes are at higher risk of dementia, a secondary aim was to investigate if the associations between nitrate and dementia varied by diabetes mellitus (DM) and pre-diabetes status.

Methods

This study involved 9,149 participants aged ≥25 years from the well-characterised Australian Diabetes, Obesity, and Lifestyle (AusDiab) Study followed over a period of 17 years. Intakes of plant-sourced, vegetable-sourced, naturally occurring animal-sourced nitrate, and processed meat (where nitrate is an allowed additive)-sourced nitrate were assessed from a 74-item food frequency questionnaire completed by participants at baseline and nitrate databases were used to estimate nitrate from these different dietary sources. Associations between source-dependent nitrate intake and dementia-related mortality were assessed using multivariable-adjusted Cox proportional hazards models adjusted for demographics, lifestyle, and dietary factors.

Results

Over 17 years of follow-up, 93 (1.0%) dementia-related deaths occurred of 1,237 (13.5%) total deaths. In multivariable-adjusted models, participants with the highest intakes of plant-sourced nitrate (median intake 98 mg/day) had a 57% lower risk of dementia-related mortality [HR (95% CI): 0.43 (0.22, 0.87)] compared to participants with lowest intakes of plant-sourced nitrate (median intake 35 mg/day). A 66% lower risk was also seen for higher intakes of vegetable-sourced nitrate [HR (95% CI): 0.34 (0.17, 0.66)]. No association was observed for animal-sourced nitrate, but the risk was two times higher amongst those who consumed the most processed meat-sourced nitrate intake [HR (95%): 2.10 (1.07, 4.12)]. The highest intake of vegetable-sourced nitrate was associated with a lower risk of dementia-related mortality for those with and without DM and pre-diabetes.

Conclusion

Encouraging the intake of nitrate-rich vegetables, such as green leafy vegetables and beetroot, may lower the risk of dementia-related mortality, particularly in individuals with (pre-) diabetes who are at a higher dementia risk.
A novel modified penile disassembly procedure for isolated male epispadias repair: preliminary outcomes
Authors: Deng Fuming , Fu Wen , Liu Guochang , Sun Liangzhong
Published: August 2024

Purpose

This study aimed to evaluate the preliminary outcome of a novel modification of a penile disassembly procedure.

Methods

We retrospectively reviewed the data of 15 patients with isolated male epispadias (IME) who underwent a modified penile disassembly procedure. This modification involved leaving the glans connected while dissecting the urethral plate from the corpus cavernosum. This approach reserves the bundles of the anastomosis at the glans, which can prevent ischemic changes.

Results

One of the first two patients experienced glans ischemic changes on the first day after the operation, which ultimately resulted in the loss of half of the hemiglans. Urethral plate shortening was observed in two patients and was addressed with a transverse preputial island flap. Two patients developed a penopubic urethral fistula, which was repaired 6 months postoperatively. Of the 15 patients, 10 (66%) were continent or social continent, while 4 were incontinent and needed bladder neck reconstruction. One patient had not yet reached the age for continence evaluation. Additionally, Two patients had a residual dorsal curvature of approximately 10–15° and were advised to undergo continuous follow-up.

Conclusions

The modified penile disassembly procedure is a simple, effective, and safe method for IME repair with an acceptable penile appearance and complication rate.
Different care mode alter composition and function of gut microbiota in cerebral palsy children
Authors: Lyu Jinli , Zhang Xiaowei , Xiong Shenghua , Wu Hui , Han Jing , Xie Yongjie , Qiu Feifeng , Yang Zhenyu , Huang Congfu
Published: August 2024

Introduction

Specialized care is essential for the recovery of children with cerebral palsy (CP). This study investigates how different care modes impact the gut microbiota.

Methods

Fecal samples from 32 children were collected, among whom those cared for by family (n = 21) were selected as the observation group, and those cared for by children's welfare institutions (n = 11) were selected as the control group (registration number of LGFYYXLL-024). The gut microbiota profiles were analyzed.

Results

There was no significant difference in the α-diversity of the gut microbiota and the abundance at the phylum level. However, at the genus level, the observation group showed a significant increase in the abundance of butyrate-producing bacteria Bacteroides and Lachnospiracea incertae sedis (P < 0.05), and a significant decrease in the abundance of opportunistic pathogens Prevotella, Clostridium cluster IV, Oscillibacter, and Fusobacterium (P < 0.05). Additionally, lipid metabolism, carbohydrate metabolism, transcription, cellular processes and signaling, and membrane transport were significantly upregulated in the observation group. Lipid metabolism was positively correlated with Bacteroides and Lachnospiracea incertae sedis, indicating a positive impact of the family-centered care mode on bacterial metabolism processes.

Discussion

This study highlights that the family-centered care mode had a positive impact on the composition and function of the gut microbiota. The study provides valuable insights into the relationship between care mode and gut microbiota, which can inspire the development of interventions for cerebral palsy.
Lawyer-client relationship in divorce proceedings: development and validation of a new instrument
Authors: Martínez-Pampliega Ana , Cormenzana Susana , García-Garnica Ma Carmen , Pellón-Elexpuru Inés , Merino Laura
Published: August 2024

Introduction

This study is based on the paradigm of collaborative law and the current absence of instruments that evaluate the lawyer-client relationship as a function of the needs of the family system. The objective was to construct and validate an instrument, conceptualizing the lawyer-client relationship as a helping relationship.

Method

Two groups of experts and 239 parents (58% mothers and 42% fathers), users of Family Visitation Centers, participated in the study. The content, construct, and criterion validity of the instrument, as well as its invariance for both parents, were analyzed.

Results

The resulting 12-item instrument has been shown to have a two-dimensional structure, invariant for both parents, with high psychometric solidity.

Discussion

The LCR scale seems to be a valuable and effective measure for use in a legal context, with important correlations with the parents’ psychological well-being, leading to a promising and relevant instrument for the holistic approach to the divorce process.
Mendelian randomization analysis does not reveal a causal influence between keratoconus and three major mental disorders
Authors: Zhang Xueyan , Wang Qiaoling , Cui Fenghua , Wu Xuelian , Zhang Chenming
Published: August 2024

Background

Observational studies have suggested at a possible link between keratoconus (KC) and various mental disorders, but the exact direction of causation in these associations remains unclear. This study aims to investigate the potential causal link between KC and three prominent mental conditions: Anxiety, Depression, and Schizophrenia.

Methods

Using instrumental variables identified from Genome-wide association study (GWAS) data of European individuals, we conducted bidirectional two-sample Mendelian Randomization (MR) analyses to explore potential causal relationships between KC and the three major mental disorders. We primarily employed the Inverse-Variance Weighted (IVW) method to evaluate causality. In addition, we performed four supplementary MR methods (MR-Egger, Weighted Median, Simple Mode, and Weighted Mode). Furthermore, we conducted various sensitivity analyses to assess heterogeneity, horizontal pleiotropy, and result stability.

Results

Our findings did not reveal any concrete evidence of a causal link between KC and the three major mental disorders, namely anxiety, depression, and schizophrenia [anxiety: odds ratio (OR)=0.997, 95% confidence interval (CI)=0.988–1.008, p = 0.621; depression: OR=1.008, 95% CI=0.999–1.017, p = 0.084; schizophrenia: OR=1.002, 95% CI= 0.984–1.020, p = 0.840]. Similarly, the three major mental disorders were not caustically associated with KC [anxiety: OR=1.014, 95% CI=0.635–1.620, p = 0.953; depression: OR=1.109, 95% CI= 0.749–1.643, p = 0.604; schizophrenia: OR= 0.969, 95% CI= 0.884–1.062, p = 0.497]. The sensitivity analyses indicated that the results remained robust, with no signs of pleiotropy or heterogeneity.

Conclusions

Our study does not support a genetically determined significant causal connection between KC and the three major mental disorders. The increased occurrence of mental disorders observed in KC patients in observational reports likely arises from factors that can be modified. Further research is warranted to unveil the underlying mechanisms behind the associations observed in observational studies.
Fostering social health of people with dementia: evaluation of the Razem przed siebie dementia awareness campaign in Poland
Authors: Błaszkiewicz M. , Szcześniak D. , Ciułkowicz M. , Rymaszewska Julia Ewa , Low L.-F. , Brodaty H. , Rymaszewska J.
Published: August 2024

Background

Due to the need to increase social awareness about dementia and the needs of patients living with dementia in Poland, the Razem przed siebie (eng. Forward with Dementia) campaign was created. The aim of the study was to evaluate its effectiveness.

Methods

To disseminate key campaign messages to the target audiences (people with dementia, carers, health and social care professionals [HSCP] and general public) a website, social and traditional media promotions, webinars and social activities were created. The campaign ran between September 2021 and April 2022. Mixed methods (online survey, reach estimates and interviews) were used to evaluate the campaign.

Results

Almost 1,300 people visited the website during the campaign period. Of these, 55 carers and HSCP responded to the online survey. The most read section of the website was Understanding the diagnosis (carers [56% of 25] and HSCP [80% out of 30]). The website was mostly accessed by carers (68%) and HSCP (66.7%) through word-of-mouth recommendations. 80% carers and 90% HSCP found the website very or extremely helpful. Over 90% of carers and HSCP expressed an intention to revisit the website. Based on 31 interviews, campaign effects, change mechanisms and limitations were identified. Campaign events elicited positive emotions among people with dementia, providing them with a feeling of belonging and engagement. Esteeming personal interactions over informational campaign materials, those with dementia felt acknowledged and empowered by the events. Carers also reported positive experiences and increased interest and knowledge, though they expressed disappointment with the lack of respite care, an issue beyond the campaign’s scope. HSCP perceived the campaign events positively and identified significant gaps in the dementia care system.

Conclusion

Evaluation of the Razem przed siebie campaign revealed successes and limitations. While effectively incorporating anti-stigma campaign recommendations and enhancing social health for individuals with dementia, the campaign clearly showed the pressing need for systemic solutions. Despite positive perception of the campaign, there is a need for a better diagnostic and post-diagnostic support for people with dementia and their carers.
Review on reverse electrodialysis process-a pioneering technology for energy generation by salinity gradient
Authors: Gül Taha Furkan , Akalın Minel , Dönmezler Eda Nur , Bolat Ahmet , Cihanoğlu Aydın , Güler Enver , Kabay Nalan
Published: August 2024

Blue energy obtained by salinity gradient can be generated by mixing two saline solutions having different salt concentrations. According to researchers working in this area, about 80% of the current global electricity demand could potentially be covered by this energy source. There are basically two membrane technologies so-called pressure-retarded osmosis (PRO) and reverse electrodialysis (RED) that are capable to generate electrical energy from salinity gradient. The pressure driven PRO process is more suitable for energy generation from highly concentrated brines. However, RED is more favorable for power generation by mixing seawater and river water. In RED process, ion exchange membranes (IEMs) placed between two electrodes in a stack were employed for transport of ions. Thus, an electrical current is obtained at the electrodes by electron transport through redox reactions. This review gives an overview of RED as a pioneering technology for salinity gradient energy (SGE) generation. The review summarizes the recent improvements of IEMs employed for RED studies, membrane fouling and RED stack design.
Quantum entanglement of optical photons: the first experiment, 1964–67
Authors: Kocher Carl A.
Published: August 2024

The first experimental observation of entangled visible light was achieved by optically exciting free atoms of calcium and detecting pairs of photons emitted in a two-stage cascade. The polarizations of the entangled photons were observed to be correlated, in agreement with quantum theory. This review describes the rationale, methodology, challenges, and results, including experimental details not previously published.
Identification of distinct physical activity profiles through adolescence: a longitudinal qualitative description study
Authors: Bélanger Mathieu , Goguen Julie , Beauchamp Jacinthe , Gallant François , Boucher Anika , Chevarie Jean-Sébastien , DeGrâce Sara , Saheb Yanis , Gagnon Maryse , Doré Isabelle , Sabiston Catherine M.
Published: August 2024

Introduction

We aimed to better understand longitudinal physical activity experiences among initially active adolescents and to identify and describe distinct physical activity profiles.

Methods

A sample of 23 physically active participants [52% female; mean age = 12.2 (0.6) years at study inception] were selected from the MATCH study to take part in this nested qualitative descriptive study. Participants were interviewed once a year for six years. Following individual-level analyses, profiles were identified based on similarity of longitudinal experiences.

Results

Four profiles captured participants' experiences: Independents (those who progressively seek activities that cater to their pursuit of autonomy); Multitaskers (those who participate in many different sports as an integral part of their lifestyle); Specialists (those who are dedicated to becoming the best they can be at one sport); Undecided (those who take part in physical activity to occupy time).

Discussion

The exploration of longitudinal physical activity experiences led to the identification of distinct profiles that could be targets for tailored interventions, theory development, and participation models.
The presentation pattern and surgical strategies in bronchopulmonary carcinoid tumors: a multicenter experience in a low-income country
Authors: Deme Workneh Tesfaye , Merine Seyoum Kassa , Wadaja Desalegn Fekadu , Gemeda Abdela Hayato , Demissie Meklit Tamrat , Bahta Mahlet Tesfaye , Reta Demissie Wondu
Published: August 2024

Background

Bronchopulmonary carcinoid tumors include typical and atypical carcinoids, with typical carcinoids accounting for 80%–90% of these types of tumor. The primary curative treatment for these tumors is surgical resection. To our knowledge, there are limited studies on the presentation patterns and treatment strategies of bronchopulmonary carcinoid tumors in Africa.

Objective

To determine the presentation patterns and surgical strategies in bronchopulmonary carcinoid tumors in patients treated at multicenters in Ethiopia from January 2018 to December 2023.

Materials and methods

A 5-year retrospective cross-sectional study was conducted using medical records and pathology record reviews of patients operated on in Tikur Anbessa Specialized Hospital, Menelik II Hospital, and Saint Peter's Specialized Hospital from 1 January 2018 to 31 December 2023. The completeness of the data was checked before being entered into EpiData version 4.6.1, and analysis was conducted using SPSS version 29. Logistic regression was applied to depict the association of the histological pattern with its predictors. A P-value of <0.05 was considered significant for the association of variables.

Results

A total of 62 patients with bronchopulmonary carcinoid tumors were included in the study with a mean age of 35.29 ± 12.26 years ranging from 14 to 67 years, in which more than half [37 (56.5%)] were females, with a male-to-female ratio of 1:1.3. The majority of the patients were non-smokers (90.3%) and symptomatic (98.4%), with a mean duration of symptoms of 29.7 ± 26 months, ranging from 3 to 156 months. Nearly half of the patients (48.4%) were treated for pulmonary tuberculosis before a diagnosis of carcinoid tumor was made. The majority of the patients underwent surgery by open posterolateral thoracotomy (98.4%), and pneumonectomy was the most common (38.7%) resection performed. Typical carcinoids were observed in 85.5% of patients. Age, smoking history, duration of symptoms, location of tumors, and lymph node status were statistically associated with histological patterns.

Recommendation

Based on our study findings, improving physician awareness on the clinical presentation of carcinoid tumors, training for surgeons in less invasive surgical approaches, and further nationwide studies are recommended.
Effect of allogeneic pure platelet-rich plasma, sodium cloxacillin, and their combination for the treatment of subclinical mastitis in crossbred cows
Authors: López Catalina , Duque-Madrid Paulo Cesar , Ceballos-Márquez Alejandro , Carmona Jorge U.
Published: August 2024

Introduction

Bovine subclinical mastitis (SCM) caused by Gram-positive bacteria is a major cause of economic loss in the dairy industry, exacerbated in situations where antimicrobial resistance is present. Pure platelet-rich plasma (P-PRP) may be a therapeutic alternative for SCM, when used alone or with antibiotics, such as sodium cloxacillin (SC). This study aimed 1) to evaluate the therapeutic efficacy of allogeneic P-PRP, SC, and their combination (P-PRP+SC) in cows with SCM caused by Staphylococcus aureus and by streptococci (Staphylococcus aureus and S. dysgalactiae); 2) to determine the concentrations of somatic cells (SCC), interleukin 1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α) and TGF-β1 in milk samples of the cows.

Methods

130 cows from 4 dairy herds completed the study, of which 40 cows were treated with P-PRP (10 mL), 28 cows with SC (5g), 36 with P-PRP+SC (10mL/5g), and 26 did not receive no treatment (negative control group, NCG).

Results

The overall bacteriological cure was observed in 10/40 (25%) cows in the P-PRP group, 9/28 (32.14%) animals in the SC group, 26/36 (72.22%) cows in the P-PRP+SC group, and 10/26 (38.46%) animals in the NCG. SCM caused by S. aureus (82/130, 63.08%), was cured in 6/24 (25%) cows treated with P-PRP, 7/24 (29.2%) cows treated with SC, 8/16 (50%) animals treated with P-PRP+SC, and in 8/18 (44.4%) cows in NCG. For SCM caused by the streptococci (48/130, 36.91%), the cure was achieved in 4/12 (33.3%) cows treated with P-PRP, 2/4 (50%) cows treated with SC, 18/20 (90%) cows treated with P-PRP+SC, and in 2/8 (25%) cows of the NCG. SCC was significantly (p < 0.001) affected by the treatment, herd, cure, bacteria group, and number of calvings factors. IL-1β milk concentrations were significantly (p < 0.001) influenced by treatment and farm factors, and the interaction between these factors. TNF-α milk concentrations were significantly (p < 0.001) influenced by time factor. TGF-β1 milk concentrations were significantly affected by the time and cure factors.

Conclusion

The combination of P-PRP and SC showed the best therapeutic response (90%) against bovine SCM caused by streptococci. However, none of the treatments showed an effective therapeutic response against S. aureus.
Case report: Gross total resection of a primary fourth ventricular meningioma using the telovelar approach in a dog
Authors: Jeong Jaemin , Lee Haebeom , Rho Yoonho , Jeon YoungJin
Published: August 2024

An 11-year-old spayed female Maltese dog presented with a 2-month history of gait alterations, wide-based stance, and chronic vomiting. Neurological examination revealed cerebellovestibular signs, including head tilt, nystagmus, strabismus, intentional tremor, and hypermetric gait. MRI showed a mass with iso- to hypointensity on T1-weighted (T1W) images and heterogeneous hyperintensity on T2-weighted (T2W) images, with marked non-uniform contrast enhancement. The tumor was removed via a telovelar approach without intraoperative complications. Postoperatively, the dog developed non-ambulatory paraparesis with the rigidity of the pelvic limbs but recovered ambulation within 6 days. Preoperative neurological signs progressively improved, and the patient was discharged without complications 10 days after surgery. Histological examination revealed dense spindle cells with an abundant collagen matrix and oval-shaped nucleated cells with small whorls, leading to a diagnosis of transitional meningioma of the fourth ventricle. MRI follow-up at 8 months postoperatively showed no definitive evidence of recurrence. At the final follow-up, 15.4 months postoperatively, mild neurological signs, including a slight head tilt and subtle strabismus, remained, but the rest of the neurological examination was normal. This is the first reported case of a meningioma in the fourth ventricle of a dog successfully removed using the telovelar approach.
Transcriptomic analysis of Penaeus monodon in response to acute and chronic hypotonic stress
Authors: Ji Jing , Wang Qiaohuang , Li Shuigen , Chen Yanting , Zhang Jiexin , Yu Hanxiu , Xu Jinzhen , Li Miaomiao , Zheng Renhao , Lin Nan , Zhang Ziping
Published: August 2024

To investigate the different mechanisms of Penaeus monodon in response to acute and chronic hypotonic stress, RNA sequencing technology was employed to profile the gene expression patterns in the gill, hepatopancreas, and hemocyte at 0, 6, 48, and 72 h post acute hypotonic stress treatment (with salinity immediately decreased from 20 psu to 4 psu) and at 0, 2, 10, 15 days during chronic hypotonic stress treatment (with salinity gradually decreased from 20 psu to 4 psu). The control group (SC) was maintained at a constant salinity of 20 psu. Differentially expressed genes (DEGs) were identified, followed by further validation using real-time quantitative reverse transcription PCR (RT-qPCR). A total of 34,217 genes were expressed through sequencing. Compared with the control group, 8,503 DEGs were identified in the acute hypotonic stress group, comprising 3,266 up-regulated and 5,237 down-regulated genes. In the chronic hypotonic stress group, 8,900 DEGs were detected, including 3,019 up-regulated and 5,881 down-regulated genes. Gene Ontology (GO) functional annotation analysis indicated that DEGs were primarily enriched in biological processes such as cellular and metabolic processes, cellular components like membrane and other cellular components, and molecular functions including structural binding and catalytic activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that DEGs were predominantly concentrated in five major pathways: metabolism, genetic information processing, environmental information processing, cellular processes, and biological systems. These pathways encompassed antigen processing and presentation, the NOD-like receptor signaling pathway, the Toll-like receptor signaling and cell apoptosis. The RT-qPCR validation of 11 DEGs (hsp70, hsp90, nlrp3, mincle, nlrp12, tlr4, myd88, imd, casp7, casp9 and toll) demonstrated that the trends observed in the quantitative results were consistent with those from the transcriptome analysis, thereby validating the reliability of transcriptome sequencing data. This study identified that hypotonic stress triggers physiological responses in P. monodon to both acute and chronic hypotonic conditions, offering valuable insights into the expression patterns of functional genes in the gills, hepatopancreas, and hemocytes of P. monodon under such stress. These findings provide foundational data and a theoretical basis for further research into the regulatory mechanism of P. monodon in response to hypotonic stress.
Synergistic Pushing and Grasping for Enhanced Robotic Manipulation Using Deep Reinforcement Learning
Authors: Shiferaw Birhanemeskel Alamir , Agidew Tayachew F. , Alzahrani Ali Saeed , Srinivasagan Ramasamy
Published: August 2024

In robotic manipulation, achieving efficient and reliable grasping in cluttered environments remains a significant challenge. This study presents a novel approach that integrates pushing and grasping actions using deep reinforcement learning. The proposed model employs two fully convolutional neural networks—Push-Net and Grasp-Net—that predict pixel-wise Q-values for potential pushing and grasping actions from heightmap images of the scene. The training process utilizes deep Q-learning with a reward structure that incentivizes both successful pushes and grasps, encouraging the robot to create favorable conditions for grasping through strategic pushing actions. Simulation results demonstrate that the proposed model significantly outperforms traditional grasp-only policies, achieving an 87% grasp success rate in cluttered environments, compared to 60% for grasp-only approaches. The model shows robust performance in various challenging scenarios, including well-ordered configurations and novel objects, with completion rates of up to 100% and grasp success rates as high as 95.8%. These findings highlight the model’s ability to generalize to unseen objects and configurations, making it a practical solution for real-world robotic manipulation tasks.
Designing a Robotic Gripper Based on the Actuating Capacity of NiTi-Based Shape Memory Wires
Authors: Teodoriu Adrian Petru , Pricop Bogdan , Lohan Nicoleta-Monica , Popa Mihai , Comăneci Radu Ioachim , Doroftei Ioan , Bujoreanu Leandru-Gheorghe
Published: August 2024

In the present study, the capacity of two commercial NiTi and NiTiCu shape memory alloy (SMA) wires to develop work-generating (WG) and constrained-recovery (CR) shape memory effects (SMEs), as well as the capacity of a commercial NiTiFe super-elastic wire to act as cold-shape restoring element, have been investigated. Using differential scanning calorimetry (DSC), the reversible martensitic transformation to austenite of the three NiTi-based wires under study was emphasized by means of an endothermic minimum of the heat flow variation with temperature. NiTi and NiTiCu wire fragments were further tested for both WG-SME and CR-SME developed during the heating, from room temperature (RT) to different maximum temperatures selected from the DSC thermograms. The former tests revealed the capacity to repetitively lift various loads during repetitive heating, while the latter tests disclosed the repetitive development of shrinkage stresses during the repetitive heating of elongated wires. The tensile behavior of the three NiTi-based SMA wires was analyzed by failure and loading–unloading tests. The study disclosed the actuation capacity of NiTi and NiTiCu shape memory wires, which were able to develop work while being heated, as well as the resetting capacity of NiTiFe super-elastic wires, which can restore the initial undeformed shape of shape memory wires which soften while being cooled down. These features enable the design of a robotic gripper based on the development of NiTi-based actuators with repetitive action.
Load Torque Component Extraction and Analysis of Ultra-High-Speed Electric Air Compressors for Fuel Cell Vehicles
Authors: Zhou Jiaming , Shu Xing , Zhang Jinming , Yi Fengyan , Hu Donghai , Zhang Caizhi , Li Yanzhao
Published: August 2024

An ultra-high-speed electric air compressor (UHSEAC) generates a load torque component during operation, leading to unstable output. As air passes through the UHSEAC, the air density changes significantly, resulting in increased uneven distribution. This causes rapid changes in gas parameters, making them difficult to extract. Consequently, the behavior of load torque component variations under ultra-high-speed operating conditions (UHSOC) remains unclear. The influence of UHSOC on the load torque component has not been studied. In this article, the fluid model of the UHSEAC is established and verified, and the load torque component is extracted based on this model. The influence of speed and torque on the load torque component under UHSOC is then studied. The results show that the frequency of the load torque component increases by 14.29% as the speed increases from 70,000 rpm to 90,000 rpm. Additionally, as the torque increases from 1.3 Nm to 1.7 Nm, the amplitude of the load torque component increases by 69.56%. These findings provide valuable guidance for future scholars seeking to optimize UHSEAC design to reduce load torque components.
Can Marandu Grass (Urochloa brizantha) Extract Modulate Methanogenesis and Rumen Microbiota?
Authors: Freitas Rafaela Scalise Xavier de , Silva Janaina Silveira da , Alves Teresa Cristina , Oliveira Alessandra Lopes de , Bueno Ives Cláudio da Silva
Published: August 2024

Urochloa spp. are the most important grasses for ruminants in Brazil and contain secondary metabolites, mainly saponins. Urochloa brizantha extracts (ethanolic EE and hydroalcoholic HE with 3.62 and 5.38 mg protodioscin mL−1, respectively) were developed to verify their potential as additives for ruminant nutrition. The in vitro gas production technique was used to evaluate ten treatments in a completely randomized factorial arrangement (2 × 4 + 2), where the main effects were two extracts (EE and HE); four levels (50, 100, 150, and 200 mL of the extract kg−1 of DM), plus two controls (one positive with 25 ppm of monensin and another with no additives). The extracts EXT (EE and HE) produced a higher proportion of acetate (C2) and lower propionate (C3) than CTL, reflected in a 31% higher C2:C3 ratio. However, there was no significant difference (p > 0.05) between the treatments for methane production parameters. Archaea and Ruminococcus’ relative gene expressions were higher in EE than in HE; however, the protozoa opposite occurred, HE was higher than EE. Fibrobacter succinogenes were 33% lower in EXT than in CTL. The addition of these extracts in a sheep diet increased the production of SCFA and decreased Fibrobacter succinogenes without altering the methane and archaeal population.
Impact of Fertilization and Seasonal Changes on Paddy Soil: Unveiling the Interplay between Agricultural Practices, Enzyme Activity, and Gene Diversity
Authors: Chen Yu-Pei , Huang Hsi-Yuan , Tsai Chia-Fang , Young Chiu-Chung
Published: August 2024

Climate change and soil acidification are critical factors affecting crop production and soil quality. This study comprehensively analyzed the impact of fertilization practices, including conventional (CA), sustainable (SA), and unfertilized (BK), on soil properties, enzyme activities, and gene diversity in paddy fields across seasonal changes. Soil pH was significantly influenced by fertilization, with higher pH in BK and a decrease in pH with increased fertilization. Soil enzyme activities and Biolog EcoPlate™ analysis revealed the lowest activities in September, with the highest in December under different practices. Metagenomic analysis showed the highest genetic richness in CA soil, with seasonal variations influencing genetic diversity. From the perspective of genes in species taxonomy, Sorangium cellulosum and Anaeromyxobacter sp. were the most abundant taxa. Soil genes annotated by CAZy, COG, and GO databases revealed highly similar gene structures among different practices. Moreover, the genetic origins of soil enzymes were linked to specific bacterial contributors. While not all gene’s diversity and abundance were associated with soil enzyme activity, arylsulfatase showed an obvious correlation. Enzyme activities proved more sensitive indicators of microbial activity than gene abundance. This study emphasizes the need for rational fertilization strategies to maintain soil enzyme activities, considering agricultural practices and seasonal variations.
Design and Experimental Testing of Potato Drip Irrigation Tape Recycling Machine
Authors: Ji Xinjie , Yan Jianguo , Xie Shengshi
Published: August 2024

The purpose of this paper is the design of a potato drip irrigation tape recycling machine that would not require manual assistance or suffer from broken tapes. To clarify the recycling performance of the new potato drip irrigation tape recycling machine under different working parameters, a single-factor performance experiment was conducted. To investigate the reasons for variations in the performance indexes of the machine under different operating parameters, RecurDyn-EDEM coupled simulation experiments were carried out, with the peak height of soil and the maximum bending angle of the drip irrigation tape during the recycling process as the experimental indexes. To determine the optimal working parameter combinations and to clarify the effects of the interaction of various factors on the performance indexes, a response surface experiment was conducted. Following this, an analysis of variance was performed, and a mathematical regression model was established based on the experimental results. The findings revealed that a machine forward speed of 2.4 km/h, a shovel angle of 12°, and a conveyor sprocket speed of 270 r/min resulted in a drip irrigation tape recovery rate of 95.2%, meeting the relevant standards for drip irrigation tape recycling operations.
Improved Tunicate Swarm Optimization Based Hybrid Convolutional Neural Network for Classification of Leaf Diseases and Nutrient Deficiencies in Rice (Oryza)
Authors: Jesie R. Sherline , Premi M. S. Godwin
Published: August 2024

In Asia, rice is the most consumed grain by humans, serving as a staple food in India. The yield of rice paddies is easily affected by nutrient deficiencies and leaf diseases. To overcome this problem and improve the yield productivity of rice, nutrient deficiency and leaf disease identification are essential. The main nutrient elements in paddies are potassium, phosphorus, and nitrogen (PPN), the deficiency of any of which strongly affects the rice plants. When multiple nutrient elements are deficient, the leaf color of the rice plants is altered. To overcome this problem, optimal nutrient delivery is required. Hence, the present study proposes the use of Fuzzy C Means clustering (FCM) with Improved Tunicate Swarm Optimization (ITSO) to segment the lesions in rice plant leaves and identify the deficient nutrients. The proposed ITSO integrates the Tunicate Swarm Optimization (TSO) and Bacterial Foraging Optimization (BFO) approaches. The Hybrid Convolutional Neural Network (HCNN), a deep learning model, is used with ITSO to classify the rice leaf diseases, as well as nutrient deficiencies in the leaves. Two datasets, namely, a field work dataset and a Kaggle dataset, were used for the present study. The proposed HCNN-ITSO classified Bacterial Leaf Bright (BLB), Narrow Brown Leaf Spot (NBLS), Sheath Rot (SR), Brown Spot (BS), and Leaf Smut (LS) in the field work dataset. Furthermore, the potassium-, phosphorus-, and nitrogen-deficiency-presenting leaves were classified using the proposed HCNN-ITSO in the Kaggle dataset. The MATLAB platform was used for experimental analysis in the field work and Kaggle datasets in terms of various performance measures. When compared to previous methods, the proposed method achieved the best accuracies of 98.8% and 99.01% in the field work and Kaggle datasets, respectively.
Genome-Wide Identification, Characterization, and Expression Profile of PDCB Gene Family in Zea mays L.
Authors: Guo Jiabao , Wang Shiji , Zhang Meichun , Song Xiaohan , Wang Hongyan
Published: August 2024

The plant kingdom harbors the Plasmodesmata Callose Binding Protein (PDCB) gene family, which plays essential roles in plant growth, development, environmental adaptation, and yield. PDCB genes are closely involved in regulating cell-to-cell communication and controlling callose deposition at plasmodesmata (PD) throughout the whole plant. Remarkably, their functions remain largely unknown in many crops, including maize. This study sought to identify the members of the PDCB gene family within the maize genome and analyze their physicochemical properties and expression patterns. Utilizing bioinformatics methodologies, a comprehensive genome-wide analysis of the PDCB gene family was performed. The findings revealed that PDCB genes were highly abundant in maize, with a total of 56 PDCB genes identified and categorized into six distinct groups. Members of the PDCB family were dispersed across all chromosomes. The PDCBs within each group exhibited significant similarity in their conserved motifs and gene structures; all members contained the X8 domain, comprising one to five exons, while displaying a straightforward genomic structure. Numerous cis-acting elements associated with plant growth and development, light response, stress-associated responses, and plant hormones were identified in the promoter regions of PDCB genes. Moreover, the PDCBs exhibited diverse expression patterns across various tissues. This study improves the comprehension of the PDCB gene family and provides a robust foundation for further research on maize.