bluuebunny/crossref_metadata_2025 huggingface dataset:
Combining Methods to Better Estimate Total Fish Richness on Temperate Reefs: The Case of a Mediterranean Coralligenous Cliff
Authors: Soldo Alen , Glavičić Igor , Kovačić Marcelo
Published: June 2021

Coralligenous habitat is considered as one of the most important special habitat types in the Mediterranean; however, due to its inaccessibility, little is known about it, although it is considered as one of the Mediterranean’s richest habitats in terms of species. Due to a low number of studies, it was presumed that the richness of coralligenous fish assemblages is underestimated using traditional visual census methods which are not applicable to the deep, steep, and vertical slopes of coralligenous cliffs and do not capture exhaustively cryptobenthic species commonly found in this habitat. This paper aims at producing a more complete assessment of fish assemblages on a coralligenous cliff by combining different methods, particularly the deep vertical transect visual census and square with anesthetics method. A total of 76 fish species were recorded on a single coralligenous cliff, supporting the opinion that coralligenous cliffs are important Mediterranean biodiversity hotspots. The analysis of species traits between species recorded by the different methods showed how complementary they are to better describe species compositions. Hence, the result of this study demonstrates that the combined use of methods is essential for a more exhaustive description of the whole fish community structure and for accurate estimates of the abundance and diversity patterns, particularly in complex habitats such as coralligenous cliffs.
Low Glucose Mediated Fluconazole Tolerance in Cryptococcus neoformans
Authors: Bhattacharya Somanon , Oliveira Natalia Kronbauer , Savitt Anne G. , Silva Vanessa K. A. , Krausert Rachel B. , Ghebrehiwet Berhane , Fries Bettina C.
Published: June 2021

Chronic meningoencephalitis is caused by Cryptococcus neoformans and is treated in many parts of the world with fluconazole (FLC) monotherapy, which is associated with treatment failure and poor outcome. In the host, C. neoformans propagates predominantly under low glucose growth conditions. We investigated whether low glucose, mimicked by growing in synthetic media (SM) with 0.05% glucose (SMlowglu), affects FLC-resistance. A > 4-fold increase in FLC tolerance was observed in seven C. neoformans strains when minimum inhibitory concentration (MIC) was determined in SMlowglu compared to MIC in SM with normal (2%) glucose (SMnlglu). In SMlowglu, C. neoformans cells exhibited upregulation of efflux pump genes AFR1 (8.7-fold) and AFR2 (2.5-fold), as well as decreased accumulation (2.6-fold) of Nile Red, an efflux pump substrate. Elevated intracellular ATP levels (3.2-fold and 3.4-fold), as well as decreased mitochondrial reactive oxygen species levels (12.8-fold and 17-fold), were found in the presence and absence of FLC, indicating that low glucose altered mitochondrial function. Fluorescence microscopy revealed that mitochondria of C. neoformans grown in SMlowglu were fragmented, whereas normal glucose promoted a reticular network of mitochondria. Although mitochondrial membrane potential (MMP) was not markedly affected in SMlowglu, it significantly decreased in the presence of FLC (12.5-fold) in SMnlglu, but remained stable in SMlowglu-growing C. neoformans cells. Our data demonstrate that increased FLC tolerance in low glucose-growing C. neoformans is the result of increased efflux pump activities and altered mitochondrial function, which is more preserved in SMlowglu. This mechanism of resistance is different from FLC heteroresistance, which is associated with aneuploidy of chromosome 1 (Chr1).
Efficient Genetic Safety Switches for Future Application of iPSC-Derived Cell Transplants
Authors: Dahlke Julia , Schott Juliane W. , Vollmer Barbosa Philippe , Klatt Denise , Selich Anton , Lachmann Nico , Morgan Michael , Moritz Thomas , Schambach Axel
Published: June 2021

Induced pluripotent stem cell (iPSC)-derived cell products hold great promise as a potential cell source in personalized medicine. As concerns about the potential risk of graft-related severe adverse events, such as tumor formation from residual pluripotent cells, currently restrict their applicability, we established an optimized tool for therapeutic intervention that allows drug-controlled, specific and selective ablation of either iPSCs or the whole graft through genetic safety switches. To identify the best working system, different tools for genetic iPSC modification, promoters to express safety switches and different safety switches were combined. Suicide effects were slightly stronger when the suicide gene was delivered through lentiviral (LV) vectors compared to integration into the AAVS1 locus through TALEN technology. An optimized HSV-thymidine kinase and the inducible Caspase 9 both mediated drug-induced, efficient in vitro elimination of transgene-positive iPSCs. Choice of promoter allowed selective elimination of distinct populations within the graft: the hOct4 short response element restricted transgene expression to iPSCs, while the CAGs promoter ubiquitously drove expression in iPSCs and their progeny. Remarkably, both safety switches were able to prevent in vivo teratoma development and even effectively eliminated established teratomas formed by LV CAGs-transgenic iPSCs. These optimized tools to increase safety provide an important step towards clinical application of iPSC-derived transplants.
Investigating the Causal Linkages among Inflation, Interest Rate, and Economic Growth in Pakistan under the Influence of COVID-19 Pandemic: A Wavelet Transformation Approach
Authors: Hayat Muhammad Azmat , Ghulam Huma , Batool Maryam , Naeem Muhammad Zahid , Ejaz Abdullah , Spulbar Cristi , Birau Ramona
Published: June 2021

This research is the earliest attempt to understand the impact of inflation and the interest rate on output growth in the context of Pakistan using the wavelet transformation approach. For this study, we used monthly data on inflation, the interest rate, and industrial production from January 1991 to May 2020. The COVID-19 pandemic has affected economies around the world, especially in view of the measures taken by governmental authorities regarding enforced lockdowns and social distancing. Traditional studies empirically explored the relationship between these important macroeconomic variables only for the short run and long run. Firstly, we employed the autoregressive distributed lag (ARDL) cointegration test and two causality tests (Granger causality and Toda–Yamamoto) to check the cointegration properties and causal relationship among these variables, respectively. After confirming the long-run causality from the ARDL bound test, we decomposed the time series of growth, inflation, and the interest rate into different time scales using wavelet analysis which allows us to study the relationship among variables for the very short run, medium run, long run, and very long run. The continuous wavelet transform (CWT), the cross-wavelet transform (XWT), cross-wavelet coherence (WTC), and multi-scale Granger causality tests were used to investigate the co-movement and nature of the causality between inflation and growth and the interest rate and growth. The results of the wavelet and multi-scale Granger causality tests show that the causal relationship between these variables is not the same across all time horizons; rather, it is unidirectional in the short-run and medium-run but bi-directional in the long-run. Therefore, this study suggests that the central bank should try to maintain inflation and the interest rate at a low level in the short run and medium run instead of putting too much pressure on these variables in the long-run.
AI Recommendation Service Acceptance: Assessing the Effects of Perceived Empathy and Need for Cognition
Authors: Yoon Namhee , Lee Ha-Kyung
Published: June 2021

This study investigated the effect of perceived technology quality and personalization quality on behavioral intentions, mediated by perceived empathy in using an artificial intelligence (AI) recommendation service. The study was based on a theoretical model of artificial intelligent device use acceptance. We also tested the moderating effect of individuals’ need for cognition, influencing empathy. Data collection was conducted through an online survey using a nationally recognized consumer research panel service in Korea. The participants were asked to respond to their preferences and needs on sneakers; then, they randomly experienced the AI (versus human expert) recommendation service that offers a recommended product. A total of 200 data were analyzed using SPSS 21.0 for descriptive statistics, reliability analysis, and PROCESS analysis, and AMOS 21.0 for confirmatory factor analysis and structural equation modeling (SEM). Results revealed that, compared with the human (expert) recommendation service, the AI recommendation service increased perceived technology quality, which increased personalization quality. Technology and personalization quality had a positive influence on behavioral intentions, mediated by perceived empathy. In addition, when individuals had a high level of need for cognition, the effect of personalization quality on empathy was stronger. However, individuals with a low level of need for cognition perceived greater empathy, as technology quality increased. The findings of the current study improve understanding of how consumers accept AI technology-driven services in the online shopping context.
iBTA-Induced Biotube® Blood Vessels: 2020 Update
Authors: Nakayama Yasuhide , Higashita Ryuji , Shiraishi Yasuyuki , Umeno Tadashi , Tajikawa Tsutomu , Yamada Akihiro , Mori Kazuki , Miyazaki Manami , Ohara Mamiko , Iwai Ryosuke , Terazawa Takeshi , Oie Tomonori , Yambe Tomoyuki , Miyamoto Shinji
Published: June 2021

Blood access is a lifeline for dialysis patients. However, serious problems such as stenosis or obstruction of access blood vessels, which are life-threatening conditions in daily clinical practice, still remain. One of the most promising candidates for solving these problems may be Biotube blood vessels. More than 20 years have passed since the development of in-body tissue architecture (iBTA), a technology for preparing tissues for autologous implantation in patients. The tissues obtained by iBTA do not elicit immunological rejection, which is one of the ultimate goals of regenerative medical engineering; however, their practical applications were quite challenging. The seemingly unorthodox iBTA concepts that do not follow the current pre-established medical system may not be readily accepted in general medicine. In contrast, there are many diseases that cannot be adequately addressed even with the latest and most advanced medical technology. However, iBTA may be able to save patients with serious diseases. It is natural that the development of high-risk medical devices that do not fit the corporate logic would be avoided. In order to actively treat such largely unattached diseases, we started Biotube Co., Ltd. with an aim to contribute to society. Biotubes induced by iBTA are collagenous tubular tissues prepared in the patient’s body for autologous implantation. The application of Biotubes as tissues for vascular implantation has been studied for many years. Biotubes may have excellent potential as small-diameter artificial blood vessels, one of the most difficult to clinically achieve. Their possibility is currently being confirmed in preclinical tests. Biotubes may save hundreds of thousands of patients worldwide annually from amputation. In addition, we aim to eliminate the recuring access vascular problems in millions of dialysis patients. This study provides an update on the current development status and future possibilities of Biotubes and their preparation molds, Biotube Makers.
The Use of Lasers in Dental Materials: A Review
Authors: Tzanakakis Emmanouil-George C. , Skoulas Evangelos , Pepelassi Eudoxie , Koidis Petros , Tzoutzas Ioannis G.
Published: June 2021

Lasers have been well integrated in clinical dentistry for the last two decades, providing clinical alternatives in the management of both soft and hard tissues with an expanding use in the field of dental materials. One of their main advantages is that they can deliver very low to very high concentrated power at an exact point on any substrate by all possible means. The aim of this review is to thoroughly analyze the use of lasers in the processing of dental materials and to enlighten the new trends in laser technology focused on dental material management. New approaches for the elaboration of dental materials that require high energy levels and delicate processing, such as metals, ceramics, and resins are provided, while time consuming laboratory procedures, such as cutting restorative materials, welding, and sintering are facilitated. In addition, surface characteristics of titanium alloys and high strength ceramics can be altered. Finally, the potential of lasers to increase the adhesion of zirconia ceramics to different substrates has been tested for all laser devices, including a new ultrafast generation of lasers.
Potential Application of an Aqueous Extract of Tinospora Cordifolia (Thunb.) Miers (Giloy) in Oral Submucous Fibrosis—An In Vitro Study
Authors: Patil Shankargouda
Published: June 2021

The in vitro antifibrotic activity of Tinospora cordifolia (Thunb.) Miers (giloy) was assessed to explore its potential for the management of oral submucous fibrosis. Epithelial cells dissociated from the tissue obtained from histopathologically normal oral mucosa during surgical extraction of third molars were cultured and fibrosis was induced by TGF-β1 in the oral keratinocytes. Cell viability was assessed by MTT and comparative gene expression analysis was carried out in the fibrosis-induced oral keratinocytes treated with various concentrations of Tinospora cordifolia extract (TcE) for matricellular protein-related gene expression. Concentrations of 0.5 µg/mL and 1 µg/mL TcE demonstrated a significant reduction in the expression of CTGF, SERPINE1, COL1A1, FN1, MMP1, MMP2, MMP3, and TIMP2 and an increase in the expression of PLAU, COL3A1, TIMP1, and TIMP3. Although TcE was found to reduce the expression of several fibrotic genes and increase the expression of antifibrotic genes, a varied effect was found, causing increased expression of COL3A1 and decreased expression of TIMP2 on TGF-β1-induced human buccal epithelial cells. However, further studies are warranted to assess the exact mechanism of antifibrotic activity and its clinical applications.
Formation and Investigation of Physicochemical, Biological and Bacteriostatic Properties of Nanocomposite Foils Containing Silver Nanoparticles and Graphene Oxide in Hyaluronic Acid Matrix
Authors: Khachatryan Karen , Khachatryan Lusine , Krzan Marcel , Krystyjan Magdalena , Krzemińska-Fiedorowicz Lidia , Lenart-Boroń Anna , Koronowicz Aneta , Drozdowska Mariola , Khachatryan Gohar
Published: June 2021

Natural polysaccharides, including hyaluronic acid, find a wide range of applications in biomedical sciences. There is a growing interest in nanocomposites containing hyaluronic acid and nanoparticles such as nanometals or graphene. In this study, we prepared foils of pure sodium hyaluronate and sodium hyaluronate containing nanosilver, graphene oxide, nanosilver/graphene oxide and characterized their properties. UV-vis spectroscopy and scanning electron microscopy (SEM) confirmed the formation of 10–20 nm silver nanoparticles. The structural changes were investigated using Fourier transforms infrared (FTIR) spectra and size exclusion chromatography. The obtained results suggest changes in molecular weights in the samples containing nanoparticles, which was highest in a sample containing nanosilver/graphene oxide. We also assessed the mechanical properties of the foils (thickness, tensile strength and elongation at break) and their wettability. The foils containing nanosilver and nanosilver/graphene oxide presented bacteriostatic activity against E. coli, Staphylococcus spp. and Bacillus spp., which was not observed in the control and sample containing graphene oxide. The composites containing graphene oxide and nanosilver/graphene oxide exhibited a cytotoxic effect on human melanoma WM266-4 cell lines (ATCC, Manassas, VA, USA).
Lignin-Based High-Performance Fibers by Textile Spinning Techniques
Authors: Jin Yanhong , Lin Jiaxian , Cheng Yu , Lu Chunhong
Published: June 2021

As a major component of lignocellulosic biomass, lignin is one of the largest natural resources of biopolymers and, thus, an abundant and renewable raw material for products, such as high-performance fibers for industrial applications. Direct conversion of lignin has long been investigated, but the fiber spinning process for lignin is difficult and the obtained fibers exhibit unsatisfactory mechanical performance mainly due to the amorphous chemical structure, low molecular weight of lignin, and broad molecular weight distribution. Therefore, different textile spinning techniques, modifications of lignin, and incorporation of lignin into polymers have been and are being developed to increase lignin’s spinnability and compatibility with existing materials to yield fibers with better mechanical performance. This review presents the latest advances in the textile fabrication techniques, modified lignin-based high-performance fibers, and their potential in the enhancement of the mechanical performance.
Biodegradable Iron-Based Materials—What Was Done and What More Can Be Done?
Authors: Gąsior Gabriela , Szczepański Jonasz , Radtke Aleksandra
Published: June 2021

Iron, while attracting less attention than magnesium and zinc, is still one of the best candidates for biodegradable metal stents thanks its biocompatibility, great elastic moduli and high strength. Due to the low corrosion rate, and thus slow biodegradation, iron stents have still not been put into use. While these problems have still not been fully resolved, many studies have been published that propose different approaches to the issues. This brief overview report summarises the latest developments in the field of biodegradable iron-based stents and presents some techniques that can accelerate their biocorrosion rate. Basic data related to iron metabolism and its biocompatibility, the mechanism of the corrosion process, as well as a critical look at the rate of degradation of iron-based systems obtained by several different methods are included. All this illustrates as the title says, what was done within the topic of biodegradable iron-based materials and what more can be done.
Comparison of Various Criteria Determining the Direction of Crack Propagation Using the UDMGINI User Procedure Implemented in Abaqus
Authors: Gontarz Jakub , Podgórski Jerzy
Published: June 2021

This paper describes a method of predicting the direction of crack propagation implemented by user subroutines in the Simulia-Abaqus FEA system with the use of the extended finite element method (X-FEM). This method is based on displacements and stresses according to Westergaard’s solution of Griffith’s crack problem. During the calculations, in each crack increment, the algorithm reads the stresses and displacements in the model around the crack tip, calculates the criterion values at the read points, reduces them to a unit distance from the crack tip, fits a polynomial to these points, and finds the minimum of the function closest to the last propagation angle. The algorithm also decides when the crack grows, depending on a chosen criterion. Four criteria have been implemented to predict the direction of failure propagation: the maximum principal stress criterion, the Ottosen–Podgórski criterion, the new criterion described here based on the minimum component values of the displacement vector, and the maximum circumferential tensile stress (MTS). These criteria were verified in two tests: the three-point bending test of the notched beam and the anchor pull-out test. For these tests, the criterion built into Simulia Abaqus does not correctly define the crack path, which causes the crack propagation direction to “rotate” when simulating the fracture. The criteria developed here, in most cases, determine the crack path and the maximum force very well compared to real laboratory tests.
Differential Action of Silver Nanoparticles on ABCB1 (MDR1) and ABCC1 (MRP1) Activity in Mammalian Cell Lines
Authors: Krzyzanowski Damian , Kruszewski Marcin , Grzelak Agnieszka
Published: June 2021

Silver nanoparticles (AgNPs), due to their unique properties have been receiving immense attention in recent years. In addition to their antibacterial and antifungal activities, AgNPs also cause apoptosis, mitochondria disfunction, nucleic acid damage and show potent anticancer properties in both multidrug resistance (MDR) and sensitive tumors. The MDR phenomenon, caused by the presence of ATP-binding cassette (ABC) proteins, is responsible for the failure of chemotherapy. Thus, investigating the influence of widely used AgNPs on ABC transporters is crucial. In the present study, we have examined the cytotoxicity of silver nanoparticles of a nominal size of 20 nm (Ag20) on the cell lines of different tissue origins. In addition, we have checked the ATP-binding cassette transporters’ activity and expression under AgNP exposure. The results indicate that Ag20 shows a toxic effect on tested cells, as well as modulating the expression and transport activity of ABC proteins.
Early-Stage Dissolution Kinetics of Silicate-Based Bioactive Glass under Dynamic Conditions: Critical Evaluation
Authors: Galusková Dagmar , Kaňková Hana , Švančárková Anna , Galusek Dušan
Published: June 2021

This manuscript presents a systematic and detailed study of ion release from 45S5 bioactive glass to develop a methodology to directly monitor dissolved ions in a simulated fluid via inductively coupled plasma optical emission spectrometry (ICP OES). For the kinetic study, two dynamic tests, an inline ICP test and a flow-through test, are performed with the same flow rate, temperature, pH, ionic strength of the solution, and sample surface to leaching solution volume ratio. The flow-through test allows for the measurement of an initial dissolution rate, as well the maximum amount of any species released from the surface of the glass. In addition, the data from the inline ICP test are obtained by immediate and direct monitoring of ions from the first minutes of contact of the glass with aqueous fluids with pH values of 4 and 7.4. The overall dissolution rates of the tested commercial bioactive glass in simulated body fluid (SBF) (pH 7.4) were significantly lower compared to the initial rate acquired. The methodology developed in this study can be applied to monitor the controlled release of ions with additional therapeutic functionalities, where the amount of ions released in the first minutes can be critical for the resulting biological performance.
Effect of the Crack Tip Bifurcation on the Plasticity-Induced Fatigue Propagation in Metallic Materials
Authors: Toribio Jesús , González Beatriz , Matos Juan-Carlos
Published: June 2021

This article deals with the influence of the crack path branching (at the micro level) on the plasticity-induced fatigue crack growth. With regard to this, a modeling by means of the finite element method was performed considering a cracked panel subjected to tension with different symmetric and asymmetric configurations of the bifurcated crack tip. The results show the appearance of a retardation effect in the growth rate of the bifurcated crack in relation to the growth rate of the fully straight crack in different cases studied, namely: (i) if the two branches of the bifurcation have different initial projected length, the propagation rate is greater at the crack tip corresponding to the long-branch than that of the short-branch, and the long-branch growth rate increases with the decrease of the initial branch angle and of the initial projected short-branch length and with the increase of the intensity of fatigue; (ii) if the two branches of the bifurcation have identical initial projected length, the retardation effect depends on the initial distance between the two bifurcated crack tips, the growth rate going up with the decrease of such a distance and with the increase of the fatigue intensity.
Substituent and Solvent Polarity on the Spectroscopic Properties in Azo Derivatives of 2-Hydroxynaphthalene and Their Difluoroboranes Complexes
Authors: Skotnicka Agnieszka , Czeleń Przemysław
Published: June 2021

Novel fluorescent dyes such as difluoroborane complexes of 1-phenylazonaphthalen-2-ol derivatives were successfully synthesized and characterized with a focus on the influence of a substituent and a solvent on the basic photophysical properties. 1H, 11B, 13C, 15N, and 19F nuclear magnetic resonance (NMR) spectra of substituted 1-phenylazonaphthalen-2-ol difluoroboranes and their parent azo dyes were recorded and discussed. The absorption and emission properties of synthesized compounds were investigated in solvents of varying polarity. They were found to be fluorescent despite the presence of the azo group. The azo group rotation was blocked by complexing with -BF2 to get a red shift in absorption. Solvent-dependent spectral properties of compounds were investigated using Lipper-Mataga and Bakhshiev plot. The calculated DFT energies and Frontier Molecular Orbitals calculations of the studied compounds were proved to be consistent with the experimental observations.
Crosslinking of Gelatin in Bicomponent Electrospun Fibers
Authors: Dulnik Judyta , Sajkiewicz Paweł
Published: June 2021

Four chemical crosslinking methods were used in order to prevent gelatin leaching in an aqueous environment, from bicomponent polycaprolactone/gelatin (PCL/Gt) nanofibers electrospun from an alternative solvent system. A range of different concentrations and reaction times were employed to compare genipin, 1-(3-dimethylaminopropyl)-N’-ethylcarbodimide hydrochloride/N-hydroxysuccinimide (EDC/NHS), 1,4-butanediol diglycidyl ether (BDDGE), and transglutaminase. The objective was to optimize and find the most effective method in terms of reaction time and solution concentration, that at the same time provides satisfactory gelatin crosslinking degree and ensures good morphology of the fibers, even after 24 h in aqueous medium in 37 °C. The series of experiments demonstrated that, out of the four compared crosslinking methods, EDC/NHS was able to yield satisfactory results with the lowest concentrations and the shortest reaction times.
Long-GRIN-Lens Microendoscopy Enabled by Wavefront Shaping for a Biomedical Microdevice: An Analytical Investigation
Authors: Liu Guigen , Kang Jeon Woong , Jonas Oliver
Published: June 2021

We analytically investigate the feasibility of long graded-index (GRIN)-lens-based microendoscopes through wavefront shaping. Following the very well-defined ray trajectories in a GRIN lens, mode-dependent phase delay is first determined. Then, the phase compensation needed for obtaining diffraction limited resolution is derived. Finally, the diffraction pattern of the lens output is computed using the Rayleigh–Sommerfeld diffraction theory. We show that diffraction-limited resolution is obtained for a 0.5 mm diameter lens with a length over 1 m. It is also demonstrated that different imaging working distances (WDs) can be realized by modifying the phase compensation. When a short design WD is used, a large imaging numerical aperture (NA) higher than 0.4 is achievable even when a low NA lens (NA = 0.1) is used. The long- and thin-GRIN-lens-based microendoscope investigated here, which is attractive for biomedical applications, is being prioritized for use in a clinical stage microdevice that measures three-dimensional drug responses inside the body. The advance described in this work may enable superior imaging capabilities in clinical applications in which long and flexible imaging probes are favored.
CA Modeling of Microsegregation and Growth of Equiaxed Dendrites in the Binary Al-Mg Alloy
Authors: Zyska Andrzej
Published: June 2021

A two-dimensional model based on the Cellular Automaton (CA) technique for simulating free dendritic growth in the binary Al + 5 wt.% alloy was presented. In the model, the local increment of the solid fraction was calculated using a methodology that takes into account changes in the concentration of the liquid and solid phase component in the interface cells during the solidification transition. The procedure of discarding the alloy component to the cells in the immediate vicinity was used to describe the initial, unstable dendrite growth phase under transient diffusion conditions. Numerical simulations of solidification were performed for a single dendrite using cooling rates of 5 K/s, 25 K/s and 45 K/s and for many crystals assuming the boundary condition of the third kind (Newton). The formation and growth of primary and secondary branches as well as the development of component microsegregation in the liquid and solid phase during solidification of the investigated alloy were analysed. It was found that with an increase in the cooling rate, the dendrite morphology changes, its cross-section and the distance between the secondary arms decrease, while the degree of component microsegregation and temperature recalescence in the initial stage of solidification increase. In order to determine the potential of the numerical model, the simulation results were compared with the predictions of the Lipton-Glicksman-Kurz (LGK) analytical model and the experimental solidification tests. It was demonstrated that the variability of the dendrite tip diameter and the growth rate determined in the Cellular Automaton (CA) model are similar to the values obtained in the LGK model. As part of the solidification tests carried out using the Derivative Differential Thermal Analysis (DDTA) method, a good fit of the CA model was established in terms of the shape of the solidification curves as well as the location of the characteristic phase transition temperatures and transformation time. Comparative tests of the real structure of the Al + 5 wt.% Mg alloy with the simulated structure were also carried out, and the compliance of the Secondary Dendrite Arm Spacing (SDAS) parameter and magnesium concentration profiles on the cross-section of the secondary dendrites arms was assessed.
Testing Procedure for Fatigue Characterization of Steel-CFRP Hybrid Laminate Considering Material Dependent Self-Heating
Authors: Mrzljak Selim , Schmidt Stefan , Kohl Andreas , Hülsbusch Daniel , Hausmann Joachim , Walther Frank
Published: June 2021

Combining carbon fiber reinforced polymers (CFRP) with steel offers the potential of utilizing the desired characteristics of both materials, such as specific strength/stiffness and fatigue strength of fiber reinforced polymers (FRP) and impact resistance of metals. Since in such hybrid laminates multiple material layers are combined, a gradual failure is likely that can lead to changes in mechanical properties. A failure of the metal partner leads to an increase in stress on the FRP, which under fatigue load results in increased self-heating of the FRP. Therefore, a suitable testing procedure is required and developed in this study, to enable a reproducible characterization of the mechanical properties under fatigue load. The resulting testing procedure, containing multiple frequency tests as well as load increase and constant amplitude tests, enabled characterization of the fatigue performance while never exceeding a testing induced change in temperature of 4 K. In addition to the development of the testing procedure, an insight into the manufacturing induced residual stresses occurring in such hybrid laminates, which impacts the load-bearing capacity, was established using finite element simulation. The gathered data and knowledge represents a basis for future in-depth investigations in the area of residual stress influence on the performance of hybrid laminates and highlights its importance, since not only the used testing procedure determines the measured fatigue performance.
A Convergent Three-Step Numerical Method to Solve a Double-Fractional Two-Component Bose–Einstein Condensate
Authors: Serna-Reyes Adán J. , Macías-Díaz Jorge E. , Reguera Nuria
Published: June 2021

This manuscript introduces a discrete technique to estimate the solution of a double-fractional two-component Bose–Einstein condensate. The system consists of two coupled nonlinear parabolic partial differential equations whose solutions are two complex functions, and the spatial fractional derivatives are interpreted in the Riesz sense. Initial and homogeneous Dirichlet boundary data are imposed on a multidimensional spatial domain. To approximate the solutions, we employ a finite difference methodology. We rigorously establish the existence of numerical solutions along with the main numerical properties. Concretely, we show that the scheme is consistent in both space and time as well as stable and convergent. Numerical simulations in the one-dimensional scenario are presented in order to show the performance of the scheme. For the sake of convenience, A MATLAB code of the numerical model is provided in the appendix at the end of this work.
Figures of Graph Partitioning by Counting, Sequence and Layer Matrices
Authors: Tomescu Mihaela Aurelia , Jäntschi Lorentz , Rotaru Doina Iulia
Published: June 2021

A series of counting, sequence and layer matrices are considered precursors of classifiers capable of providing the partitions of the vertices of graphs. Classifiers are given to provide different degrees of distinctiveness for the vertices of the graphs. Any partition can be represented with colors. Following this fundamental idea, it was proposed to color the graphs according to the partitions of the graph vertices. Two alternative cases were identified: when the order of the sets in the partition is relevant (the sets are distinguished by their positions) and when the order of the sets in the partition is not relevant (the sets are not distinguished by their positions). The two isomers of C28 fullerenes were colored to test the ability of classifiers to generate different partitions and colorings, thereby providing a useful visual tool for scientists working on the functionalization of various highly symmetrical chemical structures.
Mortality Predictors in Patients Diagnosed with COVID-19 in the Emergency Department: ECG, Laboratory and CT
Authors: Türkay Kunt Aslı , Kozaci Nalan , Torun Ebru
Published: June 2021

Background and Objectives: The aim of this study was to investigate parameters that can be used to predict mortality in patients diagnosed with COVID-19 in the emergency department (ED). Materials and Methods: Patients diagnosed with COVID-19 in the ED were included in this prospective study. The patients were divided into two groups. The surviving patients were included in Group 1 (survivors), and the patients who died were included in Group 2 (non-survivors). The electrocardiogram (ECG), laboratory results and chest computerized tomography (CCT) findings of the two groups were compared. The CCT images were classified according to the findings as normal, mild, moderate and severe. Results: Of the 419 patients included in the study, 347 (83%) survived (survivor) and 72 (17%) died (non-survivor). The heart rate and respiratory rate were found to be higher, and the peripheral oxygen saturation (SpO2) and diastolic blood pressure (DBP) were found to be lower in the non-survivor patients. QRS and corrected QT interval (QTc) were measured as longer in the non-survivor patients. In the CCT images, 79.2% of the non-survivor patients had severe findings, while 11.5% of the survivor patients had severe findings. WBC, neutrophil, NLR, lactate, D-dimer, fibrinogen, C- Reactive Protein (CRP), urea, creatinine, creatine kinase-MB (CK-MB) and hs-Troponin I levels were found to be higher and partial pressure of carbon dioxide (PCO2), base excess (BE), bicarbonate (HCO3), lymphocyte eosinophil levels were found to be lower in non-survivor patients. The highest AUC was calculated at the SpO2 level and the eosinophil level. Conclusions: COVID-19 is a fatal disease whose mortality risk can be estimated when the clinical, laboratory and imaging studies of the patients are evaluated together in the ED. SpO2 that is measured before starting oxygen therapy, the eosinophil levels and the CT findings are all important predictors of mortality risk.
Variability Study of Bond Work Index and Grindability Index on Various Critical Metal Ores
Authors: García Gloria G. , Oliva Josep , Guasch Eduard , Anticoi Hernán , Coello-Velázquez Alfredo L. , Menéndez-Aguado Juan M.
Published: June 2021

It is a well-known fact that the value of the Bond work index (wi) for a given ore varies along with the grinding size. In this study, a variability bysis is carried out with the Bond standard grindability tests on different critical metal ores (W, Ta), ranging from coarse grinding (rod mills) to fine grinding (ball mills). The relationship between wi and grinding size did not show a clear correlation, while the grindability index (gpr) and the grinding size showed a robust correlation, fitting in all cases to a quadratic curve with a very high regression coefficient. This result suggests that, when performing correlation studies among ore grindability and rock mechanics parameters, it is advised to use the grindability index instead of the Bond work index.
Theoretical Study on Thermoelectric Properties and Doping Regulation of Mg3X2 (X = As, Sb, Bi)
Authors: Wang Xiaofang , Lu Yong , Hu Ziyu , Shao Xiaohong
Published: June 2021

For searching both high-performances and better fits for near-room temperature thermoelectric materials, we here carried out a theoretical study on thermoelectric properties and doping regulation of Mg3X2 (X = As, Sb, Bi) by the combined method of first principle calculations and semi-classical Boltzmann theory. The thermoelectric properties of n-type Mg3As2, Mg3Sb2, and Mg3Bi2 were studied, and it was found that the dimensionless figures of merit, zT, are 2.58, 1.38, 0.34, and the p-type ones are 1.39, 0.64, 0.32, respectively. Furthermore, we calculated the lattice thermal conductivity of doped structures and screened out the structures with a relatively low formation energy to study the phonon dispersion and thermal conductivity in Mg3X2 (X = As, Sb, Bi). Finally, high thermoelectric zT and ultralow thermal conductivity of these doped structures was discussed.
High-Throughput Chlorophyll and Carotenoid Profiling Reveals Positive Associations with Sugar and Apocarotenoid Volatile Content in Fruits of Tomato Varieties in Modern and Wild Accessions
Authors: Aono Yusuke , Asikin Yonathan , Wang Ning , Tieman Denise , Klee Harry , Kusano Miyako
Published: June 2021

Flavor and nutritional quality has been negatively impacted during the course of domestication and improvement of the cultivated tomato (Solanum lycopersicum). Recent emphasis on consumers has emphasized breeding strategies that focus on flavor-associated chemicals, including sugars, acids, and aroma compounds. Carotenoids indirectly affect flavor as precursors of aroma compounds, while chlorophylls contribute to sugar production through photosynthesis. However, the relationships between these pigments and flavor content are still unclear. In this study, we developed a simple and high-throughput method to quantify chlorophylls and carotenoids. This method was applied to over one hundred tomato varieties, including S. lycopersicum and its wild relatives (S. l. var. cerasiforme and S. pimpinellifolium), for quantification of these pigments in fruits. The results obtained by integrating data of the pigments, soluble solids, sugars, and aroma compounds indicate that (i) chlorophyll-abundant varieties have relatively higher sugar accumulations and (ii) prolycopene is associated with an abundance of linear carotenoid-derived aroma compounds in one of the orange-fruited varieties, “Dixie Golden Giant”. Our results suggest the importance of these pigments not only as components of fruit color but also as factors influencing flavor traits, such as sugars and aroma.
Experimental Validation of a Novel Generator of Gas Mixtures Based on Axial Gas Pulses Coupled to a Micromixer
Authors: Noël Florian , Trocquet Claire , Serra Christophe A. , Le Calvé Stéphane
Published: June 2021

In this work, a novel generator of gas mixtures previously numerically investigated and based on axial gas pulses coupled to a micromixer has been conceived, manufactured, and validated. Standard gaseous pollutant mixtures and pure nitrogen or pure air were introduced in a microdevice designed to generate alternating axial gas pulses which were downstream homogenized by means of a multi-stage modular micromixer. The dilution, and therefore the final pollutant concentration, was controlled by two parameters: the ratio between the times of each of the two gas pulses and the partial pressure of the pollutant(s) mixture added to the device. The gas mixture generator was coupled to an analyzer to monitor the concentration of aromatic pollutants. The response time was optimized to be lower than 2 min in accordance with the analytical instrument. The quantity of pollutants measured at the micromixer’s outlet increased linearly with the expected gas concentration of 3.7–100 ppb generated by this novel microfluidic generator and fitted perfectly with those obtained by a reference gas dilution bench. At 5 ppb, the precision on the concentration generated is close to that obtained with the conventional gas mixing bench, i.e., around 10%.
Influence of Bubbles Causing Cavitation on Spool Oscillation of a Direct Drive Servovalve
Authors: Ren Pengda , Wang Bin , Zhang Wei , Xie Zhigang
Published: June 2021

A direct drive servovalve has some inherent benefits over its conventional counterparts, but also has better reliability and output power. However, due to the rigid connection between the spool and the motor, which takes the place of interstage drive-by fluid, the spool oscillation is a long-standing unsolved problem. In order to study the oscillation mechanism and the influencing factors, a double-circuit direct drive servovalve was numerically simulated. An oil return valve cavity was concentrated on as the main flow domain and was used to analyze the fluid flow characteristics. Local cavitation fraction and surface average cavitation fraction were defined to evaluate the cavitation situation. The periodic growth process of bubbles in the valve cavity was obtained. The numerical results show that bubbles in the oil return valve cavity changes, although the occurrence, evolution, and collapse stages were certain. The intensity of pressure pulsation caused by bubble variation is highly related to the bubbles causing the cavitation, which suggests a workable way to inhibit the spool oscillation.
Bartonella alsatica in Wild and Domestic Rabbits (Oryctolagus cuniculus) in The Netherlands
Authors: Kik Marja J. L. , Jaarsma Ryanne I. , IJzer Jooske , Sprong Hein , Gröne Andrea , Rijks Jolianne M.
Published: June 2021

Members of the genus Bartonella are Gram-negative facultative intracellular bacteria that are transmitted by arthropod vectors. Bartonella alsatica was detected in the spleens and livers of 7 out of 56 wild rabbits (Oryctolagus cuniculus) and in the liver of 1 out of 87 domestic rabbits in the Netherlands. The molecular evidence of B. alsatica infection in wild as well as domestic rabbits indicates the possibility of exposure to humans when these come in close contact with rabbits and possibly their fleas with subsequent risk of Bartonella infection and disease.
Root-Associated Bacterial Community Shifts in Hydroponic Lettuce Cultured with Urine-Derived Fertilizer
Authors: Van Gerrewey Thijs , El-Nakhel Christophe , De Pascale Stefania , De Paepe Jolien , Clauwaert Peter , Kerckhof Frederiek-Maarten , Boon Nico , Geelen Danny
Published: June 2021

Recovery of nutrients from source-separated urine can truncate our dependency on synthetic fertilizers, contributing to more sustainable food production. Urine-derived fertilizers have been successfully applied in soilless cultures. However, little is known about the adaptation of the plant to the nutrient environment. This study investigated the impact of urine-derived fertilizers on plant performance and the root-associated bacterial community of hydroponically grown lettuce (Lactuca sativa L.). Shoot biomass, chlorophyll, phenolic, antioxidant, and mineral content were associated with shifts in the root-associated bacterial community structures. K-struvite, a high-performing urine-derived fertilizer, supported root-associated bacterial communities that overlapped most strongly with control NPK fertilizer. Contrarily, lettuce performed poorly with electrodialysis (ED) concentrate and hydrolyzed urine and hosted distinct root-associated bacterial communities. Comparing the identified operational taxonomic units (OTU) across the fertilizer conditions revealed strong correlations between specific bacterial genera and the plant physiological characteristics, salinity, and NO3−/NH4+ ratio. The root-associated bacterial community networks of K-struvite and NPK control fertilized plants displayed fewer nodes and node edges, suggesting that good plant growth performance does not require highly complex ecological interactions in hydroponic growth conditions.
Dental Biofilm and Saliva Microbiome and Its Interplay with Pediatric Allergies
Authors: Arweiler Nicole B. , Rahmel Vivien , Alhamwe Bilal Alashkar , Alhamdan Fahd , Zemlin Michael , Boutin Sébastien , Dalpke Alexander , Renz Harald
Published: June 2021

Little is known about the interplay and contribution of oral microorganisms to allergic diseases, especially in children. The aim of the clinical study was to associate saliva and dental biofilm microbiome with allergic disease, in particular with allergic asthma. In a single-center study, allergic/asthmatic children (n = 15; AA-Chd; age 10.7 ± 2.9), atopic/allergic children (n = 16; AT/AL-Chd; 11.3 ± 2.9), and healthy controls (n = 15; CON-Chd; age 9.9 ± 2.2) were recruited. After removing adhering biofilms from teeth and collecting saliva, microbiome was analyzed by using a 16s-rRNA gene-based next-generation sequencing in these two mediums. Microbiome structure differed significantly between saliva and dental biofilms (β-diversity). Within the groups, the dental biofilm microbiome of AA-Chd and AT/AL-Chd showed a similar microbial fingerprint characterized by only a small number of taxa that were enriched or depleted (4) compared to the CON-Chd, while both diseased groups showed a stronger microbial shift compared to CON-Chd, revealing 14 taxa in AA-Chd and 15 taxa in AT/AL-Chd that were different. This could be the first note to the contribution of dental biofilm and its metabolic activity to allergic health or disease.