bluuebunny/crossref_metadata_2025 huggingface dataset:
A Design of All-Optical Integrated Linearized Modulator Based on Asymmetric Mach-Zehnder Modulator
Authors: Zhao Yiru , Li Jinye , Xiang Zichuan , Liu Jianguo
Published: February 2023

We proposed an all-optical linearized modulator model suitable for an integration platform. The linearized modulator is formed by an asymmetric Mach–Zehnder modulator and a carrier-separated waveguide. We focus on the suppression of both intermodulation distortion (IMD3) and second-order harmonic distortions (SHD). As a result, the third-order nonlinear terms of IMD3 and second-order nonlinear terms of SHD are entirely suppressed, leading to a spurious free dynamic range of IMD3 (SFDRIMD3) improvement of ~14 dB and SFDRSHD improvement of ~3 dB compared with a standard MZM model. The influence of large RF input signals, losses, and fabrication deviations are also discussed to prove the feasibility of the model.
Spiral Shaped Photonic Crystal Fiber-Based Surface Plasmon Resonance Biosensor for Cancer Cell Detection
Authors: Mittal Shweta , Saharia Ankur , Ismail Yaseera , Petruccione Francesco , Bourdine Anton V. , Morozov Oleg G. , Demidov Vladimir V. , Yin Juan , Singh Ghanshyam , Tiwari Manish
Published: February 2023

This work presents the design and simulation of an all-optical sensor for detection of cancer cells. The proposed device is based on the surface plasmon resonance effect on a spiral shaped photonic crystal fiber structure. The finite element method (FEM) based simulations are carried out for the different cancer cells, such as HELA, Basal, Jurkat, and MDA-MB-231, MCF7, and PC12 detection. The sensor has shown the maximum sensitivity of −289 RIU−1 for the refractive index of the detection of breast cancer cell with the resolution of 2.33 × 10−4. The sensor is effective for the refractive index range of 1.36 to 1.401.The structure is based on spiral shaped photonic crystal fiber, and has shown promising linear sensing response to support the practical feasibility of the device. The proposed sensor design is effective in detecting cervical cancer, skin cancer, blood cancer, breast cancer type 1, breast cancer type 2, and adrenal gland cancer.
Noise-like Pulses as a Source of Pump Energy
Authors: Kobtsev Sergey
Published: February 2023

This work analyses application particulars of noise-like pulses used as pulsed pump radiation for lasers (including Raman lasers) and amplifiers. The absence of a phase relationship between the electromagnetic field of the pump and that of the output laser radiation allows for a new application as a pump energy source of noise-like pulses, which may feature relatively high energy parameters. Questions related to this application are considered: configurations for noise-like pulse generation that hold a significant potential, specific aspects of amplification and compression, and characterisation of parameters of these pulses. Possibilities of the efficient application of noise-like pulses as pico- and nano-second pumping radiation are also discussed.
Dynamic Regulation of Grapevine’s microRNAs in Response to Mycorrhizal Symbiosis and High Temperature
Authors: Campos Catarina , Coito João Lucas , Cardoso Hélia , Marques da Silva Jorge , Pereira Helena Sofia , Viegas Wanda , Nogales Amaia
Published: February 2023

MicroRNAs (miRNAs) are non-coding small RNAs that play crucial roles in plant development and stress responses and can regulate plant interactions with beneficial soil microorganisms such as arbuscular mycorrhizal fungi (AMF). To determine if root inoculation with distinct AMF species affected miRNA expression in grapevines subjected to high temperatures, RNA-seq was conducted in leaves of grapevines inoculated with either Rhizoglomus irregulare or Funneliformis mosseae and exposed to a high-temperature treatment (HTT) of 40 °C for 4 h per day for one week. Our results showed that mycorrhizal inoculation resulted in a better plant physiological response to HTT. Amongst the 195 identified miRNAs, 83 were considered isomiRs, suggesting that isomiRs can be biologically functional in plants. The number of differentially expressed miRNAs between temperatures was higher in mycorrhizal (28) than in non-inoculated plants (17). Several miR396 family members, which target homeobox-leucine zipper proteins, were only upregulated by HTT in mycorrhizal plants. Predicted targets of HTT-induced miRNAs in mycorrhizal plants queried to STRING DB formed networks for Cox complex, and growth and stress-related transcription factors such as SQUAMOSA promoter-binding-like-proteins, homeobox-leucine zipper proteins and auxin receptors. A further cluster related to DNA polymerase was found in R. irregulare inoculated plants. The results presented herein provide new insights into miRNA regulation in mycorrhizal grapevines under heat stress and can be the basis for functional studies of plant-AMF-stress interactions.
Performance of APSIM to Simulate the Dynamics of Winter Wheat Growth, Phenology, and Nitrogen Uptake from Early Growth Stages to Maturity in Northern Europe
Authors: Kumar Uttam , Hansen Elly Møller , Thomsen Ingrid Kaag , Vogeler Iris
Published: February 2023

Performance of the APSIM (Agricultural Production Systems sIMulator) wheat model was assessed to simulate winter wheat phenology, biomass, grain yield, and nitrogen (N) uptake for its potential to optimize fertilizer applications for optimal crop growth and minimal environmental degradation. The calibration and evaluation dataset had 144 and 72 different field growing conditions (location (~7) × year (~5) × sowing date (2) × N treatment (7–13)), respectively, and included seven cultivars. APSIM simulated phenological stages satisfactorily with both model calibration and evaluation data sets with r2 of 0.97 and RMSE of 3.98–4.15 BBCH (BASF, Bayer, Ciba-Geigy, and Hoechst) scale. Simulations for biomass accumulation and N uptake during early growth stages (BBCH 28–49) were also reasonable with r2 of 0.65 and RMSE of 1510 kg ha−1, and r2 of 0.64–0.66 and RMSE of 28–39 kg N ha−1, respectively, with a higher accuracy during booting (BBCH 45–47). Overestimation of N uptake during stem elongation (BBCH 32–39) was attributed to (1) high inter-annual variability in simulations, and (2) high sensitivity of parameters regulating N uptake from soil. Calibration accuracy of grain yield and grain N was higher than that of biomass and N uptake at the early growth stages. APSIM wheat model showed high potential for optimizing fertilizer management in winter wheat cultivation in Northern Europe.
Nanopore Technology Applied to Targeted Detection of Tomato Brown Rugose Fruit Virus Allows Sequencing of Related Viruses and the Diagnosis of Mixed Infections
Authors: Abou Kubaa Raied , Amoia Serafina Serena , Altamura Giuseppe , Minafra Angelantonio , Chiumenti Michela , Cillo Fabrizio
Published: February 2023

Tomato (Solanum lycopersicum) plants from a commercial glasshouse were identified with symptoms compatible with a tomato brown rugose fruit virus (ToBRFV) infection. Reverse transcription-PCR and quantitative PCR confirmed the presence of ToBRFV. Subsequently, the same RNA sample and a second from tomato plants infected with a similar tobamovirus, tomato mottle mosaic virus (ToMMV), were extracted and processed for high-throughput sequencing with the Oxford Nanopore Technology (ONT). For the targeted detection of ToBRFV, the two libraries were synthesized by using six ToBRFV sequence-specific primers in the reverse transcription step. This innovative target enrichment technology enabled deep coverage sequencing of ToBRFV, with 30% of the total reads mapping to the target virus genome and 57% mapping to the host genome. The same set of primers applied to the ToMMV library generated 5% of the total reads mapping to the latter virus, indicating that sequencing of similar, non-target viral sequences was also allowed. Further, the complete genome of pepino mosaic virus (PepMV) was also sequenced from the ToBRFV library, thus suggesting that, even using multiple sequence-specific primers, a low rate of off-target sequencing can usefully provide additional information on unexpected viral species coinfecting the same samples in an individual assay. These results demonstrate that targeted nanopore sequencing can specifically identify viral agents and has sufficient sensitivity towards non-target organisms to provide evidence of mixed virus infections.
Crosslinked Polynorbornene-Based Anion Exchange Membranes with Perfluorinated Branch Chains
Authors: Cao Dafu , Sun Xiaowei , Gao Huan , Pan Li , Li Nanwen , Li Yuesheng
Published: February 2023

To investigate the effect of perfluorinated substituent on the properties of anion exchange membranes (AEMs), cross-linked polynorbornene-based AEMs with perfluorinated branch chains were prepared via ring opening metathesis polymerization, subsequent crosslinking reaction, and quaternization. The crosslinking structure enables the resultant AEMs (CFnB) to exhibit a low swelling ratio, high toughness, and high water uptake, simultaneously. In addition, benefiting from the ion gathering and side chain microphase separation caused by their flexible backbone and perfluorinated branch chain, these AEMs had high hydroxide conductivity up to 106.9 mS cm−1 at 80 °C even at low ion content (IEC < 1.6 meq g−1). This work provides a new approach to achieve improved ion conductivity at low ion content by introducing the perfluorinated branch chains and puts forward a referable way to prepare AEMs with high performance.
Development of Crosslinker-Free Polysaccharide-Lysozyme Microspheres for Treatment Enteric Infection
Authors: Li Shuo , Shi Li , Ye Ting , Huang Biao , Qin Yuan , Xie Yongkang , Ren Xiaoyuan , Zhao Xueqin
Published: February 2023

Antibiotic abuse in the conventional treatment of microbial infections, such as inflammatory bowel disease, induces cumulative toxicity and antimicrobial resistance which requires the development of new antibiotics or novel strategies for infection control. Crosslinker-free polysaccharide-lysozyme microspheres were constructed via an electrostatic layer-by-layer self-assembly technique by adjusting the assembly behaviors of carboxymethyl starch (CMS) on lysozyme and subsequently outer cationic chitosan (CS) deposition. The relative enzymatic activity and in vitro release profile of lysozyme under simulated gastric and intestinal fluids were investigated. The highest loading efficiency of the optimized CS/CMS-lysozyme micro-gels reached 84.9% by tailoring CMS/CS content. The mild particle preparation procedure retained relative activity of 107.4% compared with free lysozyme, and successfully enhanced the antibacterial activity against E. coli due to the superposition effect of CS and lysozyme. Additionally, the particle system showed no toxicity to human cells. In vitro digestibility testified that almost 70% was recorded in the simulated intestinal fluid within 6 h. Results demonstrated that the cross-linker-free CS/CMS-lysozyme microspheres could be a promising antibacterial additive for enteric infection treatment due to its highest effective dose (573.08 μg/mL) and fast release at the intestinal tract.
Effect of Short Fibers on Fracture Properties of Epoxy-Based Polymer Concrete Exposed to High Temperatures
Authors: Elalaoui Oussama
Published: February 2023

Recently, polymer concrete (PC) has been widely used in many civil engineering applications. PC shows superiority in major physical, mechanical, and fracture properties comparing to ordinary Portland cement concrete. Despite many suitable characteristics of thermosetting resins related to processing, the thermal resistance of polymer concrete composite is relatively low. This study aims to investigate the effect of incorporating short fibers on mechanical and fracture properties of PC under different ranges of high temperatures. Short carbon and polypropylene fibers were added randomly at a rate of 1 and 2% by the total weight of the PC composite. The exposure temperatures cycles were ranged between 23 to 250 °C. Various tests were conducted including flexure strength, elastic modulus, toughness, tensile crack opening, density, and porosity to evaluate the effect of addition of short fibers on fracture properties of PC. The results show that the inclusion of short fiber lead to an increase in the load carrying capacity of PC by an average of 24% and limits the crack propagation. On the other hand, the enhancement of fracture properties of based PC containing short fibers is vanished at high temperature (250 °C), but still more efficient than ordinary cement concrete. This work could lead to broader applications of polymer concrete exposed to high temperatures.
Polymeric Scaffolds Used in Dental Pulp Regeneration by Tissue Engineering Approach
Authors: Sugiaman Vinna K. , Jeffrey  , Naliani Silvia , Pranata Natallia , Djuanda Rudy , Saputri Rosalina Intan
Published: February 2023

Currently, the challenge in dentistry is to revitalize dental pulp by utilizing tissue engineering technology; thus, a biomaterial is needed to facilitate the process. One of the three essential elements in tissue engineering technology is a scaffold. A scaffold acts as a three-dimensional (3D) framework that provides structural and biological support and creates a good environment for cell activation, communication between cells, and inducing cell organization. Therefore, the selection of a scaffold represents a challenge in regenerative endodontics. A scaffold must be safe, biodegradable, and biocompatible, with low immunogenicity, and must be able to support cell growth. Moreover, it must be supported by adequate scaffold characteristics, which include the level of porosity, pore size, and interconnectivity; these factors ultimately play an essential role in cell behavior and tissue formation. The use of natural or synthetic polymer scaffolds with excellent mechanical properties, such as small pore size and a high surface-to-volume ratio, as a matrix in dental tissue engineering has recently received a lot of attention because it shows great potential with good biological characteristics for cell regeneration. This review describes the latest developments regarding the usage of natural or synthetic scaffold polymers that have the ideal biomaterial properties to facilitate tissue regeneration when combined with stem cells and growth factors in revitalizing dental pulp tissue. The utilization of polymer scaffolds in tissue engineering can help the pulp tissue regeneration process.
Neuroprotective Activities of New Monoterpenoid Indole Alkaloid from Nauclea officinalis
Authors: Liew Sook Yee , Mak Wen Qi , Thew Hin Yee , Khaw Kooi Yeong , Hazni Hazrina , Litaudon Marc , Awang Khalijah
Published: February 2023

Phytochemical investigation on the bark of Nauclea officinalis led to the isolation of a new monoterpenoid indole alkaloid, nauclediol. The structure of the compound was identified through extensive spectroscopic analysis. Nauclediol displayed cholinesterase-inhibitory activities towards AChE and BChE with IC50 values of 15.429 and 8.756 µM, respectively. Statistical analysis revealed that the mode of inhibition of nauclediol was non-competitive inhibitor for both AChE and BChE. Molecular docking revealed that nauclediol interacts with the choline-binding site and the catalytic triad of TcAChE and hBChE. This study also demonstrated the neuroprotective potential of nauclediol against amyloid beta-induced cytotoxicity and LPS-induced neuroinflammation activity in a dose-dependent manner.
Phytochemical Composition and Insight into Antibacterial Potential of Origanum vulgare Essential Oil from Saudi Arabia Using In Vitro and In Silico Approaches
Authors: Al kamaly Omkulthom , Alanazi Ashwag S. , Conte Raffaele , Imtara Hamada
Published: February 2023

In Saudi Arabia, Origanum vulgare is widely disseminated. In the present work, we used GC-MS analysis to identify the components of Origanum vulgare essential oil. The disc diffusion assay was used to assess the essential oil’s in vitro antibacterial efficacy against Gram-positive and Gram-negative Staphylococcus aureus and Escherichia coli. The antimicrobial efficacy against many microbial proteins, including tyrosyl-tRNA synthetase (TyrRS), DNA gyrase, and dihydrofolate reductase (DHFR), was further evaluated using molecular docking. Eighteen compounds were identified using GC-MS analysis, which constituted 99.81% of the total essential oil content. Terpinen-4-ol (34.3%), Beta-Terpineol (16.96%), 3-Epimoretenol (11.84%), and Alpha-Terpineol (3.86%) were the main substances identified. According to the antibacterial investigation, the inhibition zone against Staphylococcus aureus was 8 mm and 6 mm against Escherichia coli. High affinities were found between 3-Epimoretenol and tyrosyl-tRNA synthetase (TyrRS) and dihydrofolate reductase (DHFR) compared to positive controls (Clorobiocin, SCHEMBL2181345); the affinity values were −8.3 Kcal/mol and −9.2, respectively. The results of the present study indicate that Origanum vulgare essential oil can be used as a nutraceutical to treat infectious diseases.
Reliability of Different Nanofluids and Different Micro-Channel Configurations on the Heat Transfer Augmentation
Authors: Elbadawy Ibrahim , Alhajri Abdulaziz , Doust Mohammad , Almulla Yousef , Fayed Mohamed , Dinc Ali , Abouelela Mohamed , Mahariq Ibrahim , Al-Kouz Wael
Published: February 2023

Nanofluid, the fluid suspensions of a metallic nanoparticle, became a coolant fluid that is used when a promising enhancement in heat transfer is required. In the current study, the characteristics of fluid flow and heat transfer are numerically investigated using different nanofluids (Al2O3–H2O, TiO2–H2O, and SiO2–H2O) and different micro-channel heat sink (MCHS) configurations (rectangular, triangular, trapezoidal, and circular). In this numerical investigation, the effect of Re number ranged from 890 to 1500, and the effect of nanoparticle concentration ranged from 1% to 7% at constant heat flux q = 106 W/m2, and constant fluid inlet temperature of 288 K, were studied. The average heat transfer coefficient, h, and pressure drop, Δp, are used to quantify the fluid flow and heat transfer characteristics in each MCHS configuration and for each nanoparticle concentration. It is revealed that a better heat transfer coefficient is obtained for Al2O–H2O compared with other types of nanoparticles and pure water, such as 8.58% heat transfer coefficient improvement obtained at Re = 1500 and φ=7% more than that of pure water. It is also inferred that the maximum heat transfer coefficient is obtained by the triangular MCHS; however, it has the highest pressure drop because of the lowest hydraulic diameter.
Production of Biogas from Food Waste Using the Anaerobic Digestion Process with Biofilm-Based Pretreatment
Authors: Mohamed Ali Amina , Alam Md Zahangir , Mohamed Abdoul-latif Fatouma , Jami Mohammed Saedi , Gamiye Bouh Ibrahim , Adebayo Bello Ibrahim , Ainane Tarik
Published: February 2023

The production of biogas from food waste is a good approach to the minimization of food waste and increase in the production of renewable energy. However, the use of food waste as a feedstock for biogas production currently poses a difficulty due to an ineffective hydrolysis process, which is a pretreatment procedure and the initial step of the biogas conversion process. This restriction results from the food waste polymers’ solubilization and breakdown. This has an impact on the volume of biogas produced during the methanogenesis stage. It is essential to increase the biodegradation of organic compounds (OC) during the hydrolysis process to increase biogas generation. This study focuses on the enhancement of biogas production by the anaerobic digestion (AD) of food waste (FW). FW was hydrolyzed by the immobilized biofilm and digested anaerobically in a semi-continuous digester. Four different digesters including the control were prepared. The control digester composed of no hydrolyzed food waste had no immobilized biofilm while the other three digesters had immobilized biofilm-hydrolyzed food waste with inoculum concentrations of 10%, 30%, and 50%. The results showed that the 50% digester had the highest biogas yield of about 2000 mL/500 mL. The 10%, 30%, and control digesters had a biogas yield of 1523 mL, 753 mL, and 502 mL respectively. Thus, the analysis of total volatile solid (TVS) reduction in the digesters with 10%, 30%, and 50% inoculum and the control have increased to 43.4% for the digesters with 30% and 10%, 60% for the digester with 50% inoculum, and only 29% for the control. Total chemical demand (TCOD) removal increased to 29%, 33%, 43%, and 56% for the control, and 10%, 30%, and 50%, respectively for the inoculum-to-feed ratio. From these results, the 50% inoculum-to-feed ratio has shown the highest biogas production and highest degradation based on TVS reduction and TCOD reduction. Based on this study, the biofilm pretreatment method can be considered a promising method for the enhancement of biogas volume and biodegradation. Biogas production was high (2000 mL) for hydraulic retention time (HRT = 20) days but the HRT = 15 days was also able to produce a significant amount (1400 mL) of biogas and the 50% inoculum-to-feed ratio has shown the highest volume of biogas production.
Documentary Film as Interreligious Dialogue: A Cognitive Perspective
Authors: Lindsay Jenn
Published: February 2023

Research and personal experience affirm that watching a movie can change the way someone lives their life. Documentary storytelling is a multidimensional change agent, a digital media artifact that is rooted in real communities, real lives, and real stories. Because documentary is rooted in the human social world, watching it is a cognitively, psychologically, emotionally, socially, and politically complicated act. Thus, it is a potent medium for stimulating discourse, reflection, and behavioral change. This article explores the power of visual storytelling and positive media representation as a Parasocial-Relational form of interreligious dialogue and delves into practical application as it contemplates best practices for how filmmakers might harness that power, reviewing literature on the possible social, cognitive, and neurobiological impact of documentary. This interdisciplinary cognitive-sociological theory of change posits documentary film as a lever for increased interreligious competence because of its unique ability to disarm with visual storytelling and engaging characters, leading to a potentially reflexive experience of humanization and perceptual shift.
Loneliness, Solitude, Community: Insights from the Apophthegmata Patrum
Authors: Siladi Paul
Published: February 2023

The present article provides an urban reading of the Apophthegmata Patrum, in the broader context of the rediscovery and re-evaluation of monastic spirituality by and for the laity. On the one hand, the 21st century is considered to be the age of loneliness; on the other hand, loneliness defined the lives of the desert fathers, albeit its nuance was different. The wisdom of these elders could be a cure for contemporary loneliness, or, at least give us a set of models to navigate our loneliness.
The Politics of Relics: The Charisma of Rulers and Martyrs in the Middle Ages
Authors: Herrero Montserrat
Published: February 2023

Among the symbols used for representing power in the Middle Ages were the relics of saints and martyrs. When it came to political power, relics were one of the most cherished symbolic instruments to achieve legitimation of political power. However, no texts from the Middle Ages can be found that reflect the practice of associating relics with power. Rather, we have to assume or derive that reflection indirectly through narratives and stories around the relics present in the culture and religion of the time. This article reflects on the symbolic use of relics from a theological–political perspective: What kind of power acts through relics? What meaning of power is embodied in their political use of them? The thesis that the article will defend is that reflection on the politics of relics leads to a resignification of the idea of power in the Middle Ages, which is closely connected to the idea of charisma originating in the writings of the Apostle Paul.
Effects of Post-Fire Mulching with Loranthus europaeus Jacq. on Surface Runoff and Rainsplash Erosion in a Semi-Arid Pine Forest
Authors: Navidi Mehdi , Shafiei Abbas Banj , Alijanpour Ahmad , Pirsa Sajad , Ahmady-Birgani Hesam , Lucas-Borja Manuel Esteban , Zema Demetrio Antonio
Published: February 2023

To avoid flooding and erosion hazards, post-fire management actions are essential in Mediterranean forests after severe wildfires. In this regard, mulching is the most common action but some mulch materials, such as straw, may lead to adverse impacts in burned forests. The use of yellow mistletoe fruits (Loranthus europaeus Jacq., hereafter “LE”) for the production of biodegradable mulch and its effectiveness in post-fire hydrology have never been studied. To fill this gap, this study has evaluated surface runoff and rainsplash erosion in a pine forest in Central Eastern Spain burned by a wildfire and mulched by a mixture of LE fruits and straw (with or without adding clay particles) using a portable rainfall simulator. Compared to untreated sites, runoff increased in burned and mulched soils (by 13.6% for the mixture without clay and by 17.2% when clay was added, in the latter case significantly). This increase was mainly due to the compact layer created by mulch application on the soil surface. However, the peak flow and the time to peak were lower in mulched soils (on average by 32.7% and 60.5%, significantly only for the mulch mixture without clay), thus indicating that, in these soils, peak runoff takes longer and its maximum value is lower compared to untreated sites. Soil erosion noticeably and significantly decreased (up to 97%) in mulched areas in comparison to untreated sites without significant differences between the two mixtures. Overall, this study indicates to land managers that soil mulching with a mixture of Loranthus europaeus Jacq. and straw is an effective post-fire management action to reduce the soil erosion risk after a wildfire.
A Preliminary Predictive Model for Proliferative Lupus Nephritis in Juvenile Systemic Lupus Erythematosus
Authors: Lim Sern Chin , Chan Elaine Wan Ling , Mandal Shikriti Suprakash , Tang Swee Ping
Published: February 2023

Proliferative lupus nephritis, which is diagnosed by renal biopsy, has significant impact on the treatment choices and long-term prognosis of juvenile SLE (jSLE). Renal biopsies are however not always possible or available, thus leading to an ongoing search for alternative biomarkers. This study aimed to develop a clinical predictive machine learning model using routine standard parameters as an alternative tool to evaluate the probability of proliferative lupus nephritis (ISN/RPS Class III or IV). Data were collected retrospectively from jSLE patients seen at Selayang Hospital from 2004 to 2021. A total of 22 variables including demographic, clinical and laboratory features were analyzed. A recursive feature elimination technique was used to identify factors to predict pediatric proliferative lupus nephritis. Various models were then used to build predictive machine learning models and assessed for sensitivity, specificity and accuracy. There were 194 jSLE patients (165 females), of which 111 had lupus nephritis (54 proliferative pattern). A combination of 11 variables consisting of gender, ethnicity, fever, nephrotic state, hypertension, urine red blood cells (RBC), C3, C4, duration of illness, serum albumin, and proteinuria demonstrated the highest accuracy of 79.4% in predicting proliferative lupus nephritis. A decision-tree model performed the best with an AROC of 69.9%, accuracy of 73.85%, sensitivity of 78.72% and specificity of 61.11%. A potential clinically useful predictive model using a combination of 11 non-invasive variables to collectively predict pediatric proliferative lupus nephritis in daily practice was developed.
Eco-Friendly Valorization and Utilization of Plant Waste as a Source of Tannin for Leather Tanning
Authors: Khan Shahid Rehman , Khan Shahzad Maqsood , Khan Rafi Ullah
Published: February 2023

In the present study, bark, which is the waste part of a tree, was utilized as a source of tannin for leather tanning after its value addition. The barks of Acacia nilotica L. and Eucalyptus globulus, which are abundantly available in Pakistan, were selected in this study. Different extraction techniques including mechanical, soxhlet, reflux and ultrasonic extraction were used for the extraction of tannin from the selected barks. The medium of extraction included aqueous as well as different solvent mixtures in different ratios. The solvent mixtures used in this study were methanol-water and acetone–water. The methanol–water ratio was kept (50:50) and the acetone–water ratio was maintained at (50:50 and 70:30). In the first step, the process of extraction was optimized for solvent mixtures and extraction time by taking a fixed dose (5 g) of each bark and a fixed volume of each extracting solvent mixture (150 mL). The total phenolic contents (TPC) of the bark extracts were determined by colorimetric assay (as mg GAE/g bark), taking gallic acid as the reference standard. The tannin contents (TC) of the bark extracts were determined by using the standard hide powder method (SLC 117). It was concluded that among the different extraction techniques, and among the solvent mixtures acetone–water in ratio (70:30), ultrasonic extraction resulted in maximum extraction of tannin (196.1 and 125.2 mg/g), respectively for A. nilotica and E. globulus. FTIR analyses of the dried extracts obtained from both barks were compared with FTIR of the most commonly used commercial vegetable tanning agent in the leather industry, i.e., mimosa. Results were comparable, which confirmed the presence of condensed tannin in both barks. The extracted tannin was applied on a pickle and wet blue leather to evaluate its tanning and re-tanning capability. Mimosa, the most abundantly used vegetable tanning material in the leather industry, was applied as a reference standard on the same pickle and wet blue leather for comparison. Different physical properties, such as leather shrinkage temperature, leather softness, tensile strength, percent elongation and breaking strength, were measured to evaluate the tanning efficacy of the extracted tannins. The results showed that tannin obtained from A. nilotica showed comparable physical properties to those of mimosa. It was concluded from the results that tannin obtained from locally available bark, i.e., A. nilotica, can be utilized as a vegetable tanning agent for the leather industry.
Effect of Alkali and Membrane Area on the Simultaneous Recovery of Nitrogen and Phosphorous from Digestate by Membrane Technology and Chemical Precipitation
Authors: González-García Isabel , Riaño Berta , Molinuevo-Salces Beatriz , García-González María Cruz
Published: February 2023

Nutrient recovery from the agri-food sector waste is an increasingly recognized option within the framework of the bioeconomy. Membrane technologies and chemical precipitation are among the best valued options for their economic and practical feasibility. In this study, the combination of gas-permeable membrane (GPM) technology for the recovery of nitrogen (N) and the chemical precipitation for phosphorous (P) recovery from anaerobically digested swine manure is evaluated. This work studies the effect of the membrane area and the addition of alkali on N and P recovery efficiencies. Specifically, two different membrane area ratios (180 and 100 g of N per m2 of membrane) with and without the addition of alkali were studied. High nutrient recovery efficiencies, of 77% for N and 80% for P, were obtained after 10 days of experiment with a ratio of 180 g N per m2 of GPM and the addition of NaOH (1.5 N), along with the precipitant agent (MgCl2) for P precipitation. Hence, a combined configuration was proposed to perform an effective simultaneous recovery of N and P with the minimum amount of membrane needed in a short time.
Simultaneous Determination of Nine Phthalates in Vegetable Oil by Atmospheric Pressure Gas Chromatography with Tandem Mass Spectrometry (APGC-MS/MS)
Authors: Xiao Yongjun , Wong Wen Yee , Chan Li Yan , Yong Chee Keat , Abe Kosuke , Hancock Peter , Hird Simon
Published: February 2023

Although the use of phthalates has been restricted worldwide, they remain an issue due to health concerns. Diet is one of the most important exposure pathways for humans and due to their solubility in oil, phthalates are commonly found in edible oil and food high in fat. Gas chromatography–mass spectrometry (GC-MS) using electron ionization (EI) has been commonly used for the analysis of the phthalates in foodstuffs, including edible oil. However, this method suffers from issues with sensitivity and selectivity, as most phthalates are fragmented to generate a common phthalic anhydride fragment ion at m/z 149. The molecular ion cannot be observed due to strong fragmentation in EI. In contrast, atmospheric pressure gas chromatography (APGC) is a soft ionization technique with less fragmentation, whereby the molecular ion can be used as the precursor ion for multiple reaction monitoring (MRM). In this study, a simple and quick method for the determination of phthalates in vegetable oil using APGC-MS/MS was developed, and performance was assessed. The method was based on dilution of the oil in solvent and direct injection without the need for further cleanup. The established method was evaluated for linearity, recovery, precision, method detection limit (MDL), and method quantitation limit (MQL). The obtained MQL in vegetable oil was in the range of 0.015–0.058 mg/kg, despite limiting the injection volume to 1 µL, which is suitable for investigating dietary exposure and future proof against decreases to the regulatory limit. Finally, the developed method was successfully applied to analyze nine phthalates in eight commercially available vegetable oil.
Swift/UVOT: 18 Years of Long GRB Discoveries and Advances
Authors: Oates Sam
Published: February 2023

The Neil Gehrels Swift Observatory (Swift) has been in operation for 18 years. The Ultra-Violet/Optical Telescope (UVOT) onboard Swift was designed to capture the earliest optical/UV emission from gamma-ray bursts (GRBs), spanning the first few minutes to days after the prompt gamma-ray emission. In this article, we provide an overview of the long GRBs (whose prompt gamma-ray duration is >2 s) observed by the Swift/UVOT, and review the major discoveries that have been achieved by the Swift/UVOT over the last 18 years. We discuss where improvements have been made to our knowledge and understanding of the optical/UV emission, particularly the early optical/UV afterglow.
Astragalus Saponins, Astragaloside VII and Newly Synthesized Derivatives, Induce Dendritic Cell Maturation and T Cell Activation
Authors: Yakubogullari Nilgun , Cagir Ali , Bedir Erdal , Sag Duygu
Published: February 2023

Astragaloside VII (AST VII), a triterpenic saponin isolated from Astragalus species, shows promise as a vaccine adjuvant, as it supported a balanced Th1/Th2 immune response in previous in vivo studies. However, the underlying mechanisms of its adjuvant activity have not been defined. Here, we investigated the impact of AST VII and its newly synthesized semi-synthetic analogs on human whole blood cells, as well as on mouse bone marrow-derived dendritic cells (BMDCs). Cells were stimulated with AST VII and its derivatives in the presence or absence of LPS or PMA/ionomycin and the secretion of cytokines and the expression of activation markers were analyzed using ELISA and flow cytometry, respectively. AST VII and its analogs increased the production of IL-1β in PMA/ionomycin-stimulated human whole blood cells. In LPS-treated mouse BMDCs, AST VII increased the production of IL-1β and IL-12, and the expression of MHC II, CD86, and CD80. In mixed leukocyte reaction, AST VII and derivatives increased the expression of the activation marker CD44 on mouse CD4+ and CD8+ T cells. In conclusion, AST VII and its derivatives strengthen pro-inflammatory responses and support dendritic cell maturation and T cell activation in vitro. Our results provide insights into the mechanisms of the adjuvant activities of AST VII and its analogs, which will be instrumental to improve their utility as a vaccine adjuvant.
Dynamics of Anti-S IgG Antibodies Titers after the Second Dose of COVID-19 Vaccines in the Manual and Craft Worker Population of Qatar
Authors: Bansal Devendra , Atia Hassan , Al Badr Mashael , Nour Mohamed , Abdulmajeed Jazeel , Hasan Amal , Al-Hajri Noora , Ahmed Lina , Ibrahim Rumissa , Zamel Reham , Mohamed Almuthana , Pattalaparambil Hamad , Daraan Faisal , Chaudhry Adil , Oraby Sahar , El-Saleh Sahar , El-Shafie Sittana S. , Al-Farsi Affra Faiz , Paul Jiji , Ismail Ahmed , Al-Romaihi Hamad Eid , Al-Thani Mohammed Hamad , Doi Suhail A. R. , Zughaier Susu M. , Cyprian Farhan , Farag Elmobashar , Farooqui Habib Hasan
Published: February 2023

There is limited seroepidemiological evidence on the magnitude and long-term durability of antibody titers of mRNA and non-mRNA vaccines in the Qatari population. This study was conducted to generate evidence on long-term anti-S IgG antibody titers and their dynamics in individuals who have completed a primary COVID-19 vaccination schedule. A total of 300 male participants who received any of the following vaccines BNT162b2/Comirnaty, mRNA-1273, ChAdOx1-S/Covishield, COVID-19 Vaccine Janssen/Johnson, or BBIBP-CorV or Covaxin were enrolled in our study. All sera samples were tested by chemiluminescent microparticle immunoassay (CMIA) for the quantitative determination of IgG antibodies to SARS-CoV-2, receptor-binding domain (RBD) of the S1 subunit of the spike protein of SARS-CoV-2. Antibodies against SARS-CoV-2 nucleocapsid (SARS-CoV-2 N-protein IgG) were also determined. Kaplan–Meier survival curves were used to compare the time from the last dose of the primary vaccination schedule to the time by which anti-S IgG antibody titers fell into the lowest quartile (range of values collected) for the mRNA and non-mRNA vaccines. Participants vaccinated with mRNA vaccines had higher median anti-S IgG antibody titers. Participants vaccinated with the mRNA-1273 vaccine had the highest median anti-S-antibody level of 13,720.9 AU/mL (IQR 6426.5 to 30,185.6 AU/mL) followed by BNT162b2 (median, 7570.9 AU/mL; IQR, 3757.9 to 16,577.4 AU/mL); while the median anti-S antibody titer for non-mRNA vaccinated participants was 3759.7 AU/mL (IQR, 2059.7–5693.5 AU/mL). The median time to reach the lowest quartile was 3.53 months (IQR, 2.2–4.5 months) and 7.63 months (IQR, 6.3–8.4 months) for the non-mRNA vaccine recipients and Pfizer vaccine recipients, respectively. However, more than 50% of the Moderna vaccine recipients did not reach the lowest quartile by the end of the follow-up period. This evidence on anti-S IgG antibody titers should be considered for informing decisions on the durability of the neutralizing activity and thus protection against infection after the full course of primary vaccination in individuals receiving different type (mRNA verus non-mRNA) vaccines and those with natural infection.
Mitigation Measures to Control the Expected Mpox Outbreak in a Developing Country—Pakistani Scenario
Authors: Malik Shiza , Asghar Muhammad , Waheed Yasir
Published: February 2023

Mpox (previously named Monkeypox) is one of the neglected viral infectious diseases that remained silent for a long period before finally emerging as a threat to the healthcare system in endemic regions of the world in recent years. It has been mostly centered in African countries but has now been reported in other non-endemic regions as well. While keeping a strict eye on COVID pandemic handling, there is a need to remain concerned and alert about viral threats such as Mpox infections in the future. This situation has altered the healthcare system of endemic regions, including Pakistan, to stay vigilant against the expected Mpox outbreaks in the coming months. Though no specific cases have been reported in Pakistan, the healthcare system needs to take mitigation measures to tackle an expected threat before it arrives. This is important in order to avoid another major shock to the health care system of Pakistan. Moreover, since no specific treatment is available for Mpox, we can only rely upon mitigation measures, involving preventive and treatment strategies devised around some already in-use antiviral agents against Mpox viruses. Moreover, there is an imperative need to proactively prepare the healthcare system against Mpox outbreaks, spread awareness, and involve the public in a participatory approach to stay well prepared against any such infection. Moreover, there is a need to utilize financial sources, aids, and funds wisely, to create awareness in the public about such expected healthcare outbreaks in the future.
Parental Justifications for Not Vaccinating Children or Adolescents against Human Papillomavirus (HPV)
Authors: Rodrigues Eliza S. , Mendes Elisa D. T. , Nucci Luciana B.
Published: February 2023

Vaccination coverage against Human Papillomavirus (HPV) is low compared with uptake of other vaccines in many countries, including Brazil. The aim of this study was to examine the main reasons provided by parents or guardians of a target population that did not have the first dose of HPV vaccine in a small rural Brazilian municipality, and to verify the factors associated with the reasons for non-vaccination. This is a cross-sectional study with interviews based on the Health Belief Model (HBM), conducted with parents and guardians of 177 unvaccinated children or adolescents. The outcome of interest was the main reason for not vaccinating the child/adolescent. The exposure factors of interest were knowledge about HPV and its prevention as well as sociodemographic characteristics. The main justifications for not vaccinating were lack of information (62.2%), fear or refusal (29.9%), and logistical issues (7.9%). The justifications associated with adolescents’ sex, fear, or refusal were mentioned by 39.3% (95% CI: 28.8–50.6%) of parents or guardians of girls and by 21.5% (95% CI: 13.7–31.2%) of parents or guardians of boys. The main barrier to HPV vaccination is lack of information. Further training of health professionals in clarifying the benefits of vaccination and differentiating the risks between boys and girls could encourage uptake.
Exploring the Astrovirome of Shellfish Matrices Using Nanopore Sequencing
Authors: Beikpour Farzad , Pellegrini Francesco , Lanave Gianvito , Camero Michele , Catella Cristiana , Di Martino Barbara , Di Profio Federica , Masotti Chiara , Battistini Roberta , Serracca Laura , La Rosa Giuseppina , Martella Vito , Suffredini Elisabetta
Published: February 2023

Astroviruses are important human enteric pathogens transmissible with contaminated food and water. Astroviruses have also been identified in mammals, birds, lower vertebrates and invertebrates. The genetic diversity of human and animal astroviruses poses a challenge for diagnostics and taxonomy. As a proof of concept, we used a panastrovirus consensus primer set, able to amplify in a nested RT-PCR protocol a 400-nt-long fragment of the RNA-dependent RNA polymerase of most members of the Astroviridae family, in conjunction with a nanopore sequencing platform, to generate information on the astrovirome in filter-feeding mollusks. Amplicons generated from bivalve samples were used to generate libraries for deep sequencing. In three samples, only one unique RdRp sequence type was obtained. However, in seven samples and in three barcodes with eleven pooled samples, we identified a variety of known and unknown RdRp sequence types, in most cases distantly related to astrovirus sequences available in the databases. In total, 37 different sequence contigs were generated. Avian-origin astrovirus sequences were predominant, likely due to contamination of shellfish harvesting waters by marine birds. Astroviruses of the aquatic eco-system were also identified, whereas human astroviruses were not detected.
Feasibility and Validity of Postural Reaction Tests in the Neurological Examination in Healthy Rabbits
Authors: Tsai Chyong-Ying , Yu Pin-Huan , Huang Wei-Hsiang , Chang Ya-Pei
Published: February 2023

Neurological examination remains a fundamental step in the care of patients with neurological disorders. However, studies evaluating the feasibility and validity of neurological examination in rabbits are limited. In this study, postural reaction tests or methods commonly performed in dogs and cats were evaluated in clinically healthy rabbits, and we aimed to postulate a simplified examination list according to the results. The feasibility and validity of each test were determined and screened using a cut-off value of 90%. For the remaining tests/methods, the response rates of tests with similar neuroanatomical pathways were compared. Among the 34 healthy rabbits tested, one method of the hopping reaction (holding then lowering the rabbit quickly towards the floor with only the tested limb touching the ground), the hemi-walking test, the wheelbarrowing test, and the righting response yielded a feasibility and validity of over 90%. When comparing tests/methods with similar neuroanatomical pathways, the normal response rate of the hopping reaction was comparable to that of the hemi-walking test. We conclude that in healthy rabbits, hopping reaction tests using the method mentioned above, hemi-walking, wheelbarrowing, and righting responses are likely to be feasible postural reaction tests that yield consistent and normal responses.
Pilot Study: The Effects of Slice Parameters and the Interobserver Measurement Variability in Computed Tomographic Hepatic Volumetry in Dogs without Hepatic Disease
Authors: Kinoshita Kosuke , Kurihara Hitomi , Moore George E. , Murakami Masahiro
Published: February 2023

Manual computed tomographic (CT) hepatic volumetry is a non-invasive method for assessing liver volume. However, it is time-consuming with large numbers of slices. Reducing the slice number would expedite the process, but the effect of fewer slices on the accuracy of volumetric measurements in dogs has not been investigated. The objectives of this study were to evaluate the relationship between slice interval and the number of slices on hepatic volume in dogs using CT hepatic volumetry and the interobserver variability of CT volumetric measurements. We retrospectively reviewed medical records for dogs without evidence of hepatobiliary disease with abdominal CT from 2019 to 2020. Hepatic volumes were calculated by using all slices, and interobserver variability was calculated using the same dataset in 16 dogs by three observers. Interobserver variability was low, with a mean (±SD) percent difference in the hepatic volume of 3.3 (±2.5)% among all observers. The greatest percent differences in hepatic volume were decreased when using larger numbers of slices; the percent differences were <5% when using ≥20 slices for hepatic volumetry. Manual CT hepatic volumetry can be used in dogs to non-invasively assess liver volume with low interobserver variability, and a relatively reliable result can be acquired using ≥20 slices in dogs.