bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Omics Reveals Disrupted Immunometabolic Homeostasis and Oxidative Stress in Adipose Tissue of Dairy Cows with Subclinical Ketosis: A Sphingolipid-Centric Perspective
Authors: Zhao Huiying , Li Liuxue , Tan Jian , Wang Ying , Zhang Ao , Zhao Yuchao , Jiang Linshu
Published: May 2024

Ketosis, especially its subclinical form, is frequently observed in high-yielding dairy cows and is linked to various diseases during the transition period. Although adipose tissue plays a significant role in the development of metabolic disorders, its exact impact on the emergence of subclinical ketosis (SCK) is still poorly understood. The objectives of this study were to characterize and compare the profiling of transcriptome and lipidome of blood and adipose tissue between SCK and healthy cows and investigate the potential correlation between metabolic disorders and lipid metabolism. We obtained blood and adipose tissue samples from healthy cows (CON, n = 8, β-hydroxybutyric acid concentration < 1.2 mmol/L) and subclinical ketotic cows (SCK, n = 8, β-hydroxybutyric acid concentration = 1.2–3.0 mmol/L) for analyzing biochemical parameters, transcriptome, and lipidome. We found that serum levels of nonesterified fatty acids, malonaldehyde, serum amyloid A protein, IL-1β, and IL-6 were higher in SCK cows than in CON cows. Levels of adiponectin and total antioxidant capacity were higher in serum and adipose tissue from SCK cows than in CON cows. The top enriched pathways in whole blood and adipose tissue were associated with immune and inflammatory responses and sphingolipid metabolism, respectively. The accumulation of ceramide and sphingomyelin in adipose tissue was paralleled by an increase in genes related to ceramide biosynthesis, lipolysis, and inflammation and a decrease in genes related to ceramide catabolism, lipogenesis, adiponectin production, and antioxidant enzyme systems. Increased ceramide concentrations in blood and adipose tissue correlated with reduced insulin sensitivity. The current results indicate that the lipid profile of blood and adipose tissue is altered with SCK and that certain ceramide species correlate with metabolic health. Our research suggests that disruptions in ceramide metabolism could be crucial in the progression of SCK, exacerbating conditions such as insulin resistance, increased lipolysis, inflammation, and oxidative stress, providing a potential biomarker of SCK and a novel target for nutritional manipulation and pharmacological therapy.
Resonant Forcing by Solar Declination of Rossby Waves at the Tropopause and Implications in Extreme Events, Precipitation, and Heat Waves—Part 1: Theory
Authors: Pinault Jean-Louis
Published: May 2024

The purpose of this first article is to provide a physical basis for atmospheric Rossby waves at the tropopause to clarify their properties and improve our knowledge of their role in the genesis of extreme precipitation and heat waves. By analogy with the oceanic Rossby waves, the role played by the pycnocline in ocean Rossby waves is replaced here by the interface between the polar jet and the ascending air column at the meeting of the polar and Ferrel cell circulation or between the subtropical jet and the descending air column at the meeting of the Ferrel and Hadley cell circulation. In both cases, the Rossby waves are suitable for being resonantly forced in harmonic modes by tuning their natural period to the forcing period. Here, the forcing period is one year as a result of the variation in insolation due to solar declination. A search for cause-and-effect relationships is performed from the joint representation of the amplitude and phase of (1) the velocity of the cold or warm modulated airflows at 250 mb resulting from Rossby waves, (2) the geopotential height at 500 mb, and (3) the precipitation rate or ground air temperature. This is for the dominant harmonic mode whose period can be 1/16, 1/32, or 1/64 year, which reflects the intra-seasonal variations in the rising and falling air columns at the meeting of the polar, Ferrel, and Hadley cell circulation. Harmonics determine the duration of blocking. Two case studies referring to extreme cold and heat waves are presented. Dual cyclone–anticyclone systems seem to favor extreme events. They are formed by two joint vortices of opposite signs reversing over a period, concomitantly with the involved modulated airflows at the tropopause. A second article will be oriented toward (1) the examination of different case studies in order to ascertain the common characteristics of Rossby wave patterns leading to extreme events and (2) a map of the globe revealing future trends in the occurrence of extreme events.
Investigation of the Historical Trends and Variability of Rainfall Patterns during the March–May Season in Rwanda
Authors: Uwizewe Constance , Jianping Li , Habumugisha Théogène , Bello Ahmad Abdullahi
Published: May 2024

This study explores the spatiotemporal variability and determinants of rainfall patterns during the March to May (MAM) season in Rwanda, incorporating an analysis of teleconnections with oceanic–atmospheric indices over the period 1983–2021. Utilizing the Climate Hazards Group Infrared Precipitation with Stations (CHIRPS) dataset, the study employs a set of statistical tools including standardized anomalies, empirical orthogonal functions (EOF), Pearson correlation, the Mann–Kendall (MK) trend test, and Sen’s slope estimator to dissect the intricacies of rainfall variability, trends, and their association with large-scale climatic drivers. The findings reveal a distinct southwest to northwest rainfall gradient across Rwanda, with the MK test signaling a decline in annual precipitation, particularly in the southwest. The analysis for the MAM season reveals a general downtrend in rainfall, attributed in part to teleconnections with the Indian Ocean Sea surface temperatures (SSTs). Notably, the leading EOF mode for MAM rainfall demonstrates a unimodal pattern, explaining a significant 51.19% of total variance, and underscoring the pivotal role of atmospheric dynamics and moisture conveyance in shaping seasonal rainfall. The spatial correlation analysis suggests a modest linkage between MAM rainfall and the Indian Ocean Dipole, indicating that negative (positive) phases are likely to result in anomalously wet (dry) conditions in Rwanda. This comprehensive assessment highlights the intricate interplay between local rainfall patterns and global climatic phenomena, offering valuable insights into the meteorological underpinnings of rainfall variability during Rwanda’s critical MAM season.
ECMWF Ensemble Forecasts of Six Tropical Cyclones That Formed during a Long-Lasting Rossby Wave Breaking Event in the Western North Pacific
Authors: Elsberry Russell L. , Tsai Hsiao-Chung , Chin Wei-Chia , Marchok Timothy P.
Published: May 2024

The ECMWF‘s ensemble (ECEPS) predictions are documented for the lifecycles of six tropical cyclones (TCs) that formed during a long-lasting Rossby wave breaking event in the western North Pacific. All six TC tracks started between 20° N and 25° N, and between 136° E and 160° E. All five typhoons recurved north of 30° N, and the three typhoons that did not make landfall had long tracks to 50° N and beyond. The ECEPS weighted mean vector motion track forecasts from pre-formation onward are quite accurate, with track forecast spreads that are primarily related to initial position uncertainties. The ECEPS intensity forecasts have been validated relative to the Joint Typhoon Warning Center (JTWC) Working Best Track (WBT) intensities (when available). The key results for Tokage (11 W) were the ECEPS forecasts of the intensification to a peak intensity of 100 kt, and then a rapid decay as a cold-core cyclone. For Hinnamnor (12 W), the key result was the ECEPS intensity forecasts during the post-extratropical transition period when Hinnamnor was rapidly translating poleward through the Japan Sea. For Muifa (14 W), the key advantage of the ECEPS was that intensity guidance was provided for longer periods than the JTWC 5-day forecast. The most intriguing aspect of the ECEPS forecasts for post-Merbok (15 W) was its prediction of a transition to an intense, warm-core vortex after Merbok had moved beyond 50° N and was headed toward the Aleutian Islands. The most disappointing result was that the ECEPS over-predicted the slow intensification rate of Nanmadol (16 W) until the time-to-typhoon (T2TY), but then failed to predict the large rapid intensification (RI) following the T2TY. The tentative conclusion is that the ECEPS model‘s physics are not capable of predicting the inner-core spin-up rates when a small inner-core vortex is undergoing large RI.
Subtractive Proteomics and Reverse-Vaccinology Approaches for Novel Drug Target Identification and Chimeric Vaccine Development against Bartonella henselae Strain Houston-1
Authors: Rahman Sudais , Chiou Chien-Chun , Ahmad Shabir , Islam Zia Ul , Tanaka Tetsuya , Alouffi Abdulaziz , Chen Chien-Chin , Almutairi Mashal M. , Ali Abid
Published: May 2024

Bartonella henselae is a Gram-negative bacterium causing a variety of clinical symptoms, ranging from cat-scratch disease to severe systemic infections, and it is primarily transmitted by infected fleas. Its status as an emerging zoonotic pathogen and its capacity to persist within host erythrocytes and endothelial cells emphasize its clinical significance. Despite progress in understanding its pathogenesis, limited knowledge exists about the virulence factors and regulatory mechanisms specific to the B. henselae strain Houston-1. Exploring these aspects is crucial for targeted therapeutic strategies against this versatile pathogen. Using reverse-vaccinology-based subtractive proteomics, this research aimed to identify the most antigenic proteins for formulating a multi-epitope vaccine against the B. henselae strain Houston-1. One crucial virulent and antigenic protein, the PAS domain-containing sensor histidine kinase protein, was identified. Subsequently, the identification of B-cell and T-cell epitopes for the specified protein was carried out and the evaluated epitopes were checked for their antigenicity, allergenicity, solubility, MHC binding capability, and toxicity. The filtered epitopes were merged using linkers and an adjuvant to create a multi-epitope vaccine construct. The structure was then refined, with 92.3% of amino acids falling within the allowed regions. Docking of the human receptor (TLR4) with the vaccine construct was performed and demonstrated a binding energy of −1047.2 Kcal/mol with more interactions. Molecular dynamic simulations confirmed the stability of this docked complex, emphasizing the conformation and interactions between the molecules. Further experimental validation is necessary to evaluate its effectiveness against B. henselae.
Comprehensive Molecular Analysis of Disease-Related Genes as First-Tier Test for Early Diagnosis, Classification, and Management of Patients Affected by Nonsyndromic Ichthyosis
Authors: Fioretti Tiziana , Martora Fabrizio , De Maggio Ilaria , Ambrosio Adelaide , Piscopo Carmelo , Vallone Sabrina , Amato Felice , Passaro Diego , Acquaviva Fabio , Gaudiello Francesca , Di Girolamo Daniela , Maiolo Valeria , Zarrilli Federica , Esposito Speranza , Vitiello Giuseppina , Auricchio Luigi , Sammarco Elena , Brasi Daniele De , Petillo Roberta , Gambale Antonella , Cattaneo Fabio , Ammendola Rosario , Nappa Paola , Esposito Gabriella
Published: May 2024

Inherited ichthyoses are a group of clinically and genetically heterogeneous rare disorders of skin keratinization with overlapping phenotypes. The clinical picture and family history are crucial to formulating the diagnostic hypothesis, but only the identification of the genetic defect allows the correct classification. In the attempt to molecularly classify 17 unrelated Italian patients referred with congenital nonsyndromic ichthyosis, we performed massively parallel sequencing of over 50 ichthyosis-related genes. Genetic data of 300 Italian unaffected subjects were also analyzed to evaluate frequencies of putative disease-causing alleles in our population. For all patients, we identified the molecular cause of the disease. Eight patients were affected by autosomal recessive congenital ichthyosis associated with ALOX12B, NIPAL4, and TGM1 mutations. Three patients had biallelic loss-of-function variants in FLG, whereas 6/11 males were affected by X-linked ichthyosis. Among the 24 different disease-causing alleles we identified, 8 carried novel variants, including a synonymous TGM1 variant that resulted in a splicing defect. Moreover, we generated a priority list of the ichthyosis-related genes that showed a significant number of rare and novel variants in our population. In conclusion, our comprehensive molecular analysis resulted in an effective first-tier test for the early classification of ichthyosis patients. It also expands the genetic, mutational, and phenotypic spectra of inherited ichthyosis and provides new insight into the current understanding of etiologies and epidemiology of this group of rare disorders.
Recurrent Acute Coronary Syndrome in Young Man with Familial Hypercholesterolemia: Efficacy of Evolocumab Add-On Treatment Demonstrated via Serial Coronary Angiography
Authors: Kim Narae , Cho Jin-Man , Yang In-Ho
Published: May 2024

In patients with acute coronary syndrome (ACS), lipid-lowering therapy plays an important role in the prevention of the recurrence of cardiovascular disease. Recent guidelines recommend the use of proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors in patients with ACS if their low-density lipoprotein cholesterol (LDL-C) levels are not adequately controlled with statins and ezetimibe. Based on this, we report a case in which administering a PCSK9 inhibitor successfully lowered the patient’s LDL-C level to the target level and managed the coronary artery disease (CAD) recurrence. A 39-year-old man who was taking statins presented to the hospital with chest pain and was diagnosed with unstable angina. He started taking maximum doses of statins and ezetimibe to lower his LDL-C. However, the patient’s unstable angina recurred 1 year later, and a de novo lesion with plaque rupture was demonstrated via coronary angiography. The LDL-C failed to reach the target level despite maintaining the maximum dose of statin and ezetimibe. Accordingly, evolocumab was initiated in addition to rosuvastatin/ezetimibe 20/10 mg daily. Subsequently, coronary angiography was performed twice, and on follow-up, the patient remained free of CAD recurrence. This case highlights the efficacy of lipid-lowering therapy with evolocumab in high-risk patients with repeated ACS.
EEG Changes in Migraine—Can EEG Help to Monitor Attack Susceptibility?
Authors: van den Hoek Thomas C. , van de Ruit Mark , Terwindt Gisela M. , Tolner Else A.
Published: May 2024

Migraine is a highly prevalent brain condition with paroxysmal changes in brain excitability believed to contribute to the initiation of an attack. The attacks and their unpredictability have a major impact on the lives of patients. Clinical management is hampered by a lack of reliable predictors for upcoming attacks, which may help in understanding pathophysiological mechanisms to identify new treatment targets that may be positioned between the acute and preventive possibilities that are currently available. So far, a large range of studies using conventional hospital-based EEG recordings have provided contradictory results, with indications of both cortical hyper- as well as hypo-excitability. These heterogeneous findings may largely be because most studies were cross-sectional in design, providing only a snapshot in time of a patient’s brain state without capturing day-to-day fluctuations. The scope of this narrative review is to (i) reflect on current knowledge on EEG changes in the context of migraine, the attack cycle, and underlying pathophysiology; (ii) consider the effects of migraine treatment on EEG features; (iii) outline challenges and opportunities in using EEG for monitoring attack susceptibility; and (iv) discuss future applications of EEG in home-based settings.
Comparing Redox and Intracellular Signalling Responses to Cold Plasma in Wound Healing and Cancer
Authors: Abdo Adrian I. , Kopecki Zlatko
Published: May 2024

Cold plasma (CP) is an ionised gas containing excited molecules and ions, radicals, and free electrons, and which emits electric fields and UV radiation. CP is potently antimicrobial, and can be applied safely to biological tissue, birthing the field of plasma medicine. Reactive oxygen and nitrogen species (RONS) produced by CP affect biological processes directly or indirectly via the modification of cellular lipids, proteins, DNA, and intracellular signalling pathways. CP can be applied at lower levels for oxidative eustress to activate cell proliferation, motility, migration, and antioxidant production in normal cells, mainly potentiated by the unfolded protein response, the nuclear factor-erythroid factor 2-related factor 2 (Nrf2)-activated antioxidant response element, and the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) pathway, which also activates nuclear factor-kappa B (NFκB). At higher CP exposures, inactivation, apoptosis, and autophagy of malignant cells can occur via the degradation of the PI3K/Akt and mitogen-activated protein kinase (MAPK)-dependent and -independent activation of the master tumour suppressor p53, leading to caspase-mediated cell death. These opposing responses validate a hormesis approach to plasma medicine. Clinical applications of CP are becoming increasingly realised in wound healing, while clinical effectiveness in tumours is currently coming to light. This review will outline advances in plasma medicine and compare the main redox and intracellular signalling responses to CP in wound healing and cancer.
Short Fiber-Reinforced Polymer Polyamide 6 Lugs and Selective Laser-Melted Ti-6Al-4V Bushing Contact Cohesive Zone Model Mode II Parameters’ Evaluation
Authors: Sedelnikov Andry , Kurkin Evgenii , Smelov Vitaliy , Chertykovtseva Vladislava , Alekseev Vyacheslav , Gavrilov Andrey , Kishov Evgenii , Zvyagincev Maksim , Chernyakin Sergey
Published: May 2024

This paper discusses an approach to estimating the parameters of the cohesive zone model (CZM) by mode II by extruding the bushing along the lug axis. This method of evaluation requires small samples, which is particularly relevant when investigating short fiber-reinforced polymers (SFRPs) with additively manufactured embedded elements. Adhesion is investigated on the example of 30% carbon fiber-reinforced polyamide-6 molded to Ti-6Al-4V (VT6) selective laser-melted (SLM) alloy bushing in cases of a roughness Ra = 2.66 μm (vibratory finishing), Ra = 8.79 μm (sandblasting), and Ra = 10.02 (directly from SLM). The values of the maximum equivalent tangential contact stress were in a range from 1.1 MPa to 9.5 MPa, while the critical fracture energy for tangential slip was estimated at 15 N/mm for all cases. Experimental validation of the obtained CZM mode II was carried out by evaluating the load-carrying capacity of the lugs with different bushings. In both the experiment and the calculation, greater bushing roughness provides greater lug load-bearing capacity. The ribbed bushings added significant strength in the experiments, which confirmed the importance of considering the tangential mode in the contact model. The presented models can be used for the preliminary evaluation of short fiber-reinforced polyamide-6 parts with titanium-embedded elements bearing capacity.
Population Status and Conservation of the Largest Population of the Endangered François’ Langur (Trachypithecus francoisi) in Vietnam
Authors: Le Tu A. , Nguyen Anh T. , Le Trung S. , Le Tuan A. , Le Minh D.
Published: May 2024

François’ langur is an Endangered colobine inhabiting limestone habitats in southern China and northern Vietnam. Its global population has been estimated to be just more than 2000 mature individuals. Populations in Vietnam are highly fragmented with reportedly fewer than 200 adults in total and 50 in a single location. Although the François’ langur in Vietnam is highly imperiled as remnant populations persist in only three to four sites, little research has been carried out to provide a reliable estimate of its remaining population. In this study, we conducted field surveys in Lam Binh District, Tuyen Quang Province, northeastern Vietnam. In total, we recorded at least 16 groups of François’ langurs, with 156 individuals, raising the total number of individuals by approximately 10% compared to a previous study. The group structure, group size, activity budget, and density of the Lam Binh population resemble those reported in François’ langurs in China and other limestone langur species. The results show that the behavior ecology of limestone langurs significantly differs from that of forest langurs probably because they occupy separate habitats with distinctly different environmental variables. During our surveys, we detected a number of direct threats to this population, namely illegal logging, hunting, firewood collecting, hydropower development, grazing, and mining. It is recommended that the protection forest be elevated to the nature reserve status to better protect the most important population of the François’ langur in Vietnam.
Two New Species of Hymenogaster (Hymenogastraceae, Agaricales) from China Based on Morphological and Molecular Markers
Authors: Li Ting , Mao Ning , Fu Haoyu , Zhang Yuxin , Fan Li
Published: May 2024

Two new species from China, Hymenogaster pseudoniveus and H. zunhuaensis, are described and illustrated based on morphological and molecular evidence. Hymenogaster pseudoniveus is distinguished from other species of the genus based on the color of peridium, which is snow white to white when unexcavated but then turns yellowish to earth yellow, and the basidiospores, which are ornamented with nearly longitudinally arranged ridges up to 2 μm high. H. zunhuaensis is diagnosed by its dirty white to pale yellow peridium, yellow brown to brown gleba, and the smaller (Lm × Wm = 11.7 μm × 9.8 μm) broadly ellipsoidal to subglobose basidiospores (Q = 1.1–1.3). ITS/LSU-based phylogenetic analysis supports the erection of the two new species, each placed in distinct clades with strong statistical support, suggesting that they represent two distinct species novel to science. Based on the morphological and molecular evidence, we have published two new species of Hymenogaster. A key for Hymenogaster species from China is provided.
An Innovative Mechanical Approach to Mitigating Torque Fluctuations in IC Engines during Idle Operation
Authors: Cardoso Daniel Silva , Fael Paulo Oliveira , Gaspar Pedro Dinis , Espírito-Santo António
Published: May 2024

Internal combustion engines have been a major contributor to air pollution. Replacing these engines with electric propulsion systems presents significant challenges due to different countries’ needs and limitations. An active, purely mechanical solution to the problem of irregular torque production in an alternative internal combustion engine is proposed. This solution uses an actuator built on a camshaft and a spring, which stores and returns energy during the engine operating cycle, allowing torque production to be normalized, avoiding heavy flywheels. Designed for control throughout the engine’s duty cycle, this system incorporates a cam profile and a spring mechanism. The spring captures energy during the expansion stroke, which is then released to the engine during the intake and compression strokes. Simple, lightweight, and efficient, this system ensures smoother and more consistent engine operations. It presents a viable alternative to the heavy and problematic dual-mass flywheels that were introduced in the 1980s and are still in use. This innovative approach could significantly enhance the performance and reliability of alternative internal combustion engines without notable energy losses.
Correction: Matchim Kamden et al. A Comprehensive Study on DES Pretreatment Application to Microalgae for Enhanced Lipid Recovery Suitable for Biodiesel Production: Combined Experimental and Theoretical Investigations. Energies 2023, 16, 3806
Authors: Matchim Kamdem Michele Corneille , Tamafo Fouegue Aymard Didier , Lai Nanjun
Published: May 2024

The authors wish to make the following correction to this paper [...]
Improved Design and Economic Estimation of Cold-End Systems for Inland Nuclear Power Plants
Authors: Zhang Wenjie , Li Yushan , Liu Peiqi , Wei Huimin
Published: May 2024

Reserve sites for coastal nuclear power plants are gradually being depleted, prompting a shift towards the development of inland nuclear power stations. A new cooling system based on the integration of multiple cooling sources using a hybrid dry–wet cycle is proposed to achieve a balance between energy and water consumption for inland nuclear power stations. Comparative studies among all the available cooling systems were further conducted to analyze the cooling performance and economic viability. The case study results indicate that, in comparison to relative humidity, the cooling performance and circulating water consumption of cooling systems are more susceptible to changes in dry-bulb temperature. In arid and water-scarce regions, a Combined Natural Draft Hybrid Cooling System generally exhibits a monthly average circulating water consumption rate that is more than 270 kg/s lower than that of the natural draft wet cooling system, with an average monthly back pressure reduction of 0.11 kPa. When the dry-bulb temperature exceeds 13 °C, the net profit of wet cooling surpasses that of hybrid cooling. However, this scenario undergoes a reversal as the dry-bulb temperature decreases and local water prices rise. It is emphasized that hybrid cooling demonstrates minimal impact when subjected to changes in environmental conditions, offering extensive regional applicability.
Passive Shunted Piezoelectric Systems for Vibration Control of Wind Turbine Towers: A Feasibility Study
Authors: Daraki Maria-Styliani , Marakakis Konstantinos , Alevras Panagiotis , Foutsitzi Georgia A. , Stavroulakis Georgios E.
Published: May 2024

Many countries have a variety of offshore and onshore wind turbines that face extreme aging challenges. Issues with harmful vibrations that must be minimized are addressed in this paper. A new method of wind turbine tower vibration control using piezoelectricity and shunt circuits is proposed in this paper. The passive vibration control method is shown to improve the tower’s structural performance under various environmental loads, like wind and seismic excitations. To examine the effectiveness of the suggested shunted piezoelectric system, a simple surrogate finite element model of a wind turbine tower is considered, and various investigations at the second eigenfrequency are carried out. An alternative way of modeling the studied structure is considered and the results demonstrate better performance. The advantages of setting up structural damping systems for decreasing tower vibrational loads and boosting their structural stability and resilience against extreme events are highlighted throughout this work.
Comparative Analysis of Enzymatic Activities and Transcriptional Profiles of Various Hepatic Enzymes between Male and Female Yellowfin Tuna (Thunnus albacares)
Authors: Liu Dongge , Yang Hao , Li Shuisheng , Huang Hai , Li Guangli , Chen Huapu
Published: May 2024

Yellowfin tuna (Thunnus albacares) is a valuable pelagic migratory fish with potential for aquaculture. Despite this, there is limited understanding of the biological and physiological characteristics of this species, particularly regarding sex differences in growth performance. The liver, a crucial organ for digestion and metabolism, plays a significant role in regulating fish growth. This study aimed to compare liver enzyme activities and transcriptome profiles between female and male yellowfin tuna to uncover the molecular mechanisms underlying difference between the sexes. The results revealed that female yellowfin tuna exhibited higher amylase and lipid metabolism enzyme activities, while male yellowfin tuna showed higher glucose-6-phosphate dehydrogenase and antioxidant enzyme activities. Additionally, through Illumina sequencing technology, the study generated 37.74 Gb of clean data and identified 36,482 unique genes (UniGenes) in the liver transcriptome. A total of 2542 differentially expressed genes were found, with enriched Gene Ontology terms and pathways related to metabolic processes, particularly lipid metabolism and transport. These findings suggest that female yellowfin tuna have superior digestive enzyme activities and lipid metabolism, while male yellowfin tuna excel in sugar metabolism, ATP production, and antioxidant defense. This study provides valuable insights into sex differences in yellowfin tuna and could aid in advancing full-cycle aquaculture practices for this species.
Ultrasound-Assisted Nanoemulsion Loaded with Optimized Antibacterial Essential Oil Blend: A New Approach against Escherichia coli, Staphylococcus aureus, and Salmonella Enteritidis in Trout (Oncorhynchus mykiss) Fillets
Authors: Torres Neto Luiz , Monteiro Maria Lucia Guerra , da Silva Bruno Dutra , Machado Maxsueli Aparecida Moura , Mutz Yhan da Silva , Conte-Junior Carlos Adam
Published: May 2024

This study aimed to obtain and characterize an oil-in-water nanoemulsion (NE) loaded with an in vitro optimized bactericidal essential oil blend of 50% oregano, 40% thyme, and 10% lemongrass and to evaluate its potential at three different concentrations (0.5%, 1%, and 2%) in the inactivation of Escherichia coli, Staphylococcus aureus, and Salmonella enterica serotype Enteritidis inoculated in rainbow trout fillets stored at 4 °C for 9 days. Regarding the NE, the nanometric size (<100 nm) with low polydispersion (0.17 ± 0.02) was successfully obtained through ultrasound at 2.09 W/cm2. Considering the three concentrations used, S. Enteritidis was the most susceptible. On the other hand, comparing the concentrations used, the NE at 2% showed better activity, reducing S. Enteritidis, E. coli, and S. aureus by 0.33, 0.20, and 0.73 log CFU/g, respectively, in the trout fillets. Thus, this data indicates that this is a promising eco-friendly alternative to produce safe fish for consumption and reduce public health risks.
Investigating and Improving Pedestrian Safety in an Urban Environment of a Low- or Middle-Income Country: A Case Study of Yaoundé, Cameroon
Authors: Feudjio Steffel Ludivin Tezong , Tchaheu Dimitri Tchaheu , Fondzenyuy Stephen Kome , Jackai Isaac Ndumbe , Usami Davide Shingo , Persia Luca
Published: May 2024

In Yaoundé, Cameroon, where walking dominates transport modes, pedestrian safety remains an issue as pedestrians account for a fair share of road traffic casualties, partly due to the lack of walking policies and pedestrian facilities safety data, hindering targeted intervention. This study used a pedestrian safety index (PSI) and the Global Walkability Index (GWI) to investigate 12 road segments frequented by diverse pedestrian groups. Indexes were graded from E—lowest to A—highest and analyzed using description and rank correlation. Main safety issues included lack of adequate and accessible sidewalks, bollards, pedestrian crossings, signage, shade, and street lighting. Only one segment (R7) achieved grade C, while the remainder scored D or E, indicating poor pedestrian safety conditions and an unpleasant walking experience. The correlation coefficient (0.69) between the PSI and GWI at a 99% significance level validated the safety assessment, providing confidence in the results. A seven-year (2024–2030) safety strategy is proposed to improve all roads to grade B. This strategy contains several interventions, including engineering improvement, which have been proven effective. This study offers evidence for city officials to improve pedestrian safety and informs walking policies and the implementation of upcoming projects. Future research should quantify the recommendations’ benefits and validate indexes with crash or conflict data.
Farmer Perceptions of Land Cover Classification of UAS Imagery of Coffee Agroecosystems in Puerto Rico
Authors: Klenke Gwendolyn , Brines Shannon , Hernandez Nayethzi , Li Kevin , Glancy Riley , Cabrera Jose , Neal Blake H. , Adkins Kevin A. , Schroeder Ronny , Perfecto Ivette
Published: May 2024

Highly diverse agroecosystems are increasingly of interest as the realization of farms’ invaluable ecosystem services grows. Simultaneously, there has been an increased use of uncrewed aerial systems (UASs) in remote sensing, as drones offer a finer spatial resolution and faster revisit rate than traditional satellites. With the combined utility of UASs and the attention on agroecosystems, there is an opportunity to assess UAS practicality in highly biodiverse settings. In this study, we utilized UASs to collect fine-resolution 10-band multispectral imagery of coffee agroecosystems in Puerto Rico. We created land cover maps through a pixel-based supervised classification of each farm and assembled accuracy assessments for each classification. The average overall accuracy (53.9%), though relatively low, was expected for such a diverse landscape with fine-resolution data. To bolster our understanding of the classifications, we interviewed farmers to understand their thoughts on how these maps may be best used to support their land management. After sharing imagery and land cover classifications with farmers, we found that while the prints were often a point of pride or curiosity for farmers, integrating the maps into farm management was perceived as impractical. These findings highlight that while researchers and government agencies can increasingly apply remote sensing to estimate land cover classes and ecosystem services in diverse agroecosystems, further work is needed to make these products relevant to diversified smallholder farmers.
Cultural Competence Interventions in European Healthcare: A Scoping Review
Authors: De-María Berta , Topa Gabriela , López-González M. Angeles
Published: May 2024

Europe is undergoing rapid social change and is distinguished by its cultural superdiversity. Healthcare is facing an increasing need for professionals to adapt to this environment. Thus, the promotion of cultural competence in healthcare has become a priority. However, the training being developed and their suitability for the European context are not well known. The aim of this qualitative study has been to map the scientific literature in order to comprehend the current state of research on this topic. For this purpose, we conducted a systematic scoping review of the empirical publications focused on cultural competence interventions for healthcare professionals in European countries. The search was conducted in eight thematic (PsycINFO, MedLine, and PubPsych) and multidisciplinary databases (Academic Search Ultimate, E-Journals, Scopus, ProQuest, and Web of Science) to identify relevant publications up to 2023. Results were presented qualitatively. Out of the initial 6506 records screened, a total of 63 publications were included. Although the interventions were implemented in 23 different European countries, cultural competence interventions have not been widely adopted in Europe. Significant heterogeneity was observed in the conception and operacionalización of cultural competence models and in the implementation of the interventions. The interventions have mostly aimed at improving healthcare for minority population groups and have focused on the racial and ethnic dimensions of the individual. Future research is needed to contribute to the conceptual development of cultural competence to design programs tailored to European superdiversity. This scoping review has been registered in OSF and is available for consultation.
A Cost–Consequence Analysis of Nutritional Interventions Used in Hospital Settings for Older Adults with or at Risk of Malnutrition
Authors: Wong Alvin , Huang Yingxiao , Banks Merrilyn D. , Sowa P. Marcin , Bauer Judy D.
Published: May 2024

Background: Malnutrition is a significant and prevalent issue in hospital settings, associated with increased morbidity and mortality, longer hospital stays, higher readmission rates, and greater healthcare costs. Despite the potential impact of nutritional interventions on patient outcomes, there is a paucity of research focusing on their economic evaluation in the hospital setting. This study aims to fill this gap by conducting a cost–consequence analysis (CCA) of nutritional interventions targeting malnutrition in the hospital setting. Methods: We performed a CCA using data from recent systematic reviews and meta-analyses, focusing on older adult patients with or at risk of malnutrition in the hospital setting. The analysis included outcomes such as 30-day, 6-month, and 12-month mortality; 30-day and 6-month readmissions; hospital complications; length of stay; and disability-adjusted life years (DALYs). Sensitivity analyses were conducted to evaluate the impact of varying success rates in treating malnutrition and the proportions of malnourished patients seen by dietitians in SingHealth institutions. Results: The CCA indicated that 28.15 DALYs were averted across three SingHealth institutions due to the successful treatment or prevention of malnutrition by dietitians from 1 April 2021 to 31 March 2022, for an estimated 45,000 patients. The sensitivity analyses showed that the total DALYs averted ranged from 21.98 (53% success rate) to 40.03 (100% of malnourished patients seen by dietitians). The cost of implementing a complex nutritional intervention was USD 218.72 (USD 104.59, USD 478.40) per patient during hospitalization, with additional costs of USD 814.27 (USD 397.69, USD 1212.74) when the intervention was extended for three months post-discharge and USD 638.77 (USD 602.05, USD 1185.90) for concurrent therapy or exercise interventions. Conclusion: Nutritional interventions targeting malnutrition in hospital settings can have significant clinical and economic benefits. The CCA provides valuable insights into the costs and outcomes associated with these interventions, helping healthcare providers and policymakers to make informed decisions on resource allocation and intervention prioritization.
An Innovative System for Enhancing Archaeological In Situ Excavation through Geospatial Integration
Authors: Dimara Asimina , Tsakiridis Sotirios , Psarros Doukas , Papaioannou Alexios , Varsamis Dimitrios , Anagnostopoulos Christos-Nikolaos , Krinidis Stelios
Published: May 2024

The field of archaeological excavation has seen enormous developments as a result of the quick development of digital technologies. It is critical to acknowledge the long-term benefits of new approaches. In this regard, this study intends to suggest a system that provides archaeologists with digital tools that allow them to fully and effectively document their excavations in real time while in the field, which is specifically designed for classical and Byzantine archaeology. The system not only facilitates traditional documentation practices but also integrates advanced geospatial technologies and augmented reality, enhancing the accuracy and depth of archaeological research and preservation. This dual functionality enables both the efficient recording of excavation data as experienced by excavators on-site and the detailed documentation processed by researchers post-excavation. The objective of the application is to generate a sophisticated system that will enable the excavation data and experience that the excavator gains on the site of work to be recorded in real time, as well as the documentation that is subsequently processed and accomplished by the researcher or by other researchers. The system saves detailed images and 3D models of artifacts and excavation sites. This ensures that every detail is recorded while preserving the data for future analysis and reference. Lastly, the application was tested in realistic use case scenarios and real-world settings, which increased the system’s credibility and demonstrated its capability to enhance the procedures involved in archaeological excavation and documentation.
Effect of Transplanting Time and Nitrogen–Potassium Ratio on Yield, Growth, and Quality of Cauliflower Landrace Gigante di Napoli in Southern Italy
Authors: Tallarita Alessio Vincenzo , Cozzolino Eugenio , Salluzzo Antonio , Sekara Agnieszka , Pokluda Robert , Murariu Otilia Cristina , Vecchietti Lorenzo , del Piano Luisa , Lombardi Pasquale , Cuciniello Antonio , Caruso Gianluca
Published: May 2024

Research has been increasingly focusing on the preservation of the biodiversity of vegetable crops under sustainable farming management. An experiment was carried out in southern Italy on Brassica oleracea L. var. botrytis, landrace Gigante di Napoli, to assess the effects of two transplanting times (9 September and 7 October), in factorial combination with five nitrogen–potassium ratios (0.6; 0.8; 1.0; 1.2; and 1.4) on plant growth, yield, and quality of cauliflower heads. A split-plot design was used for the treatment distribution in the field, with three replications. The earlier transplant and the 1.2 N:K ratio led to the highest yield, mean weight, and firmness of cauliflower heads which were not significantly affected by both transplanting time and N:K ratio in terms of colour components. The 1.2 N:K ratio led to the highest head diameter with the earlier transplant, whereas the 1.0 ratio was the most effective on this parameter in the later crop cycle. The highest nitrate, nitrogen, and potassium concentrations in the heads were recorded with the earlier transplanting time. Antioxidant activity, ascorbic acid, and polyphenol content increased with the rise of the N:K ratio. The element use efficiency was constantly negative with the N:K increase for nitrogen and was augmented until the 1.2 ratio for potassium. The results of our investigation showed that the optimal combination between transplanting time and N:K ratio is a key aspect to improve head yield and quality of the cauliflower landrace Gigante di Napoli, under the perspective of biodiversity safeguarding and valorisation.
Tomato (Solanum lycopersicum L.) YTH Domain-Containing RNA-Binding Protein (YTP) Family Members Participate in Low-Temperature Treatment and Waterlogging Stress Responses
Authors: Zhang Yidan , Guo Tianli , Li Jingyuan , Jiang Libo , Wang Na
Published: May 2024

YT521-B homology (YTH) domain-containing RNA-binding proteins (YTPs) are important N6-methyladenosine (m6A) readers that have crucial roles in determining the destiny of m6A-modified RNAs, which are the most widespread RNA modifications in eukaryotes. Tomatoes (Solanum lycopersicum L.) hold significant importance in both dietary consumption patterns and scientific inquiries. While the YTP gene family has been characterized in tomatoes, their specific reactions to the low temperature and waterlogging stresses remain to be elucidated. In our study, nine tomato SlYTPs could be divided into five subclasses, YTHDFa-c and YTHDCa-b. After gene cloning and measuring their expression levels under stress conditions, it was revealed that SlYTP8 exhibited increased sensitivity to low-temperature treatment, while the expression levels of SlYTP9 were notably upregulated in leaf tissues subjected to waterlogging conditions. As members of the YTHDFc subfamily, SlYTP8 and SlYTP9 are both localized in the cytoplasm. Nevertheless, overexpression (OE) of SlYTP8 increased the sensitivity of tomato plants to low-temperature treatment, which was manifested by a higher accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2) and a weaker reactive oxygen species scavenging ability compared to wild-type (WT) tomatoes. However, in comparison to WT plants, the leaves of SlYTP9 OE tomatoes showed higher chlorophyll content and a stronger reactive oxygen species scavenging ability after 3 days of waterlogging treatment, thereby increasing the resistance of tomatoes to waterlogging stress. Moreover, in order to investigate the possible molecular mechanisms underlying their responses to the low temperature and waterlogging stresses, the transcription factors and interacting protein networks associated with SlYTP8/9 promoters and proteins were also predicted, respectively. These results could fill the gap in the understanding of tomato YTPs in response to the low temperature and waterlogging stresses, while also providing a theoretical and experimental basis for subsequent studies on their molecular mechanisms.
Temporal Assessment of Phosphorus Speciation in a Model Ramsar Lake System in Asia
Authors: Venukumar Anjali , Azimov Abdugani M. , Iztleuov Gani M. , Moorchilot Vishnu S. , Aravind Usha K. , Sataev Marat I. , Koshy Valsamma J. , Aravindakumar Charuvila T.
Published: May 2024

This study focused on monitoring phosphorus (P) concentrations in the water of the Ramsar site, Lake Vembanad, with a special focus on the mouths of the river bodies draining into the lake, a known hotspot for eutrophication. Four phosphorus fractions—total reactive phosphorus (TRP), total acid hydrolysable phosphorus (TAHP), total organic phosphorus (TOP), and total phosphorus (TP)—were monitored during the pre-monsoon and post-monsoon seasons. The results revealed high levels of all monitored phosphorus fractions, with an average concentration exceeding 300 ppb P across both seasons, indicating a highly eutrophic state. Notably, TRP, TOP, and TP showed high concentrations in both the pre-monsoon and post-monsoon periods. These data suggest significant phosphorus input into the lake’s surface water, potentially triggering excessive algal growth and threatening the biodiversity of this rich wetland ecosystem.
The Association between Salivary Metabolites and Gingival Bleeding Score in Healthy Subjects: A Pilot Study
Authors: Antonelli Rita , Ferrari Elena , Gallo Mariana , Ciociola Tecla , Calciolari Elena , Spisni Alberto , Meleti Marco , Pertinhez Thelma A.
Published: May 2024

Periodontal diseases, including gingivitis and periodontitis, are among the most prevalent diseases in humans. Gingivitis is the mildest form of periodontal disease, characterized by inflammation of the gingiva caused by the accumulation of dental plaque. Salivary diagnostics are becoming increasingly popular due to the variation in saliva composition in response to pathological processes. We used a metabolomics approach to investigate whether a specific saliva metabolic composition could indicate preclinical stage of gingivitis. 1H-NMR spectroscopy was used to obtain the salivary metabolite profiles of 20 healthy subjects. Univariate/multivariate statistical analysis evaluated the whole saliva metabolite composition, and the Full-Mouth Bleeding Score (FMBS) was employed as a classification parameter. Identifying a signature of specific salivary metabolites could distinguish the subjects with high FMBS scores but still within the normal range. This set of metabolites may be due to the enzymatic activities of oral bacteria and be associated with the early stages of gingival inflammation. Although this analysis is to be considered exploratory, it seems feasible to establish an FMBS threshold that distinguishes between the absence and presence of early inflammatory alterations at the salivary level.
Targeting the Multiple Complex Processes of Hypoxia-Ischemia to Achieve Neuroprotection
Authors: Maïza Auriane , Hamoudi Rifat , Mabondzo Aloïse
Published: May 2024

Hypoxic-ischemic encephalopathy (HIE) is a major cause of newborn brain damage stemming from a lack of oxygenated blood flow in the neonatal period. Twenty-five to fifty percent of asphyxiated infants who develop HIE die in the neonatal period, and about sixty percent of survivors develop long-term neurological disabilities. From the first minutes to months after the injury, a cascade of events occurs, leading to blood-brain barrier (BBB) opening, neuronal death and inflammation. To date, the only approach proposed in some cases is therapeutic hypothermia (TH). Unfortunately, TH is only partially protective and is not applicable to all neonates. This review synthesizes current knowledge on the basic molecular mechanisms of brain damage in hypoxia-ischemia (HI) and on the different therapeutic strategies in HI that have been used and explores a major limitation of unsuccessful therapeutic approaches.