bluuebunny/crossref_metadata_2025 huggingface dataset:
Reaction Mechanism of Pyrolysis and Combustion of Methyl Oleate: A ReaxFF-MD Analysis
Authors: Wei Yu , Zhang Xiaohui , Qing Shan , Wang Hua
Published: July 2024

As an emerging environmentally friendly fuel, biodiesel has excellent fuel properties comparable to those of petrochemical diesel. Oleic acid methyl ester, as the main component of biodiesel, has the characteristics of high cetane number and low emission rate of harmful gases. However, the comprehensive chemical conversion pathway of oleic acid methyl ester is not clear. In this paper, the reactive force field molecular dynamics simulation (ReaxFF-MD) method is used to construct a model of oleic acid methyl ester pyrolysis and combustion system. Further, the chemical conversion kinetics process at high temperatures (2500 K–3500 K) was studied, and a chemical reaction network was drawn. The research results show that the density of the system has almost no effect on the decomposition activation energy of oleic acid methyl ester, and the activation energies of its pyrolysis and combustion processes are 190.02 kJ/mol and 144.89 kJ/mol, respectively. Ethylene, water and carbon dioxide are the dominant and most accumulated products. From the specific reaction mechanism, the main pyrolysis path of oleic acid methyl ester is the breakage of the C-C bond to produce small molecule intermediates, and subsequent transformation of the ester group radical into carbon oxides. The combustion path is the evolution of long-chain alkanes into short-carbon-chain gaseous products, and these species are further burned to form stable CO2 and H2O. This study further discusses the microscopic combustion kinetics of biodiesel, providing a reference for the construction of biodiesel combustion models. Based on this theoretical study, the understanding of free radicals, intermediates, and products in the pyrolysis and combustion of biomass can be deepened.
Modeling the Efficiency of Biogas Plants by Using an Interval Data Analysis Method
Authors: Dyvak Mykola , Manzhula Volodymyr , Melnyk Andriy , Rusyn Bohdan , Spivak Iryna
Published: July 2024

This article considers the task of developing mathematical models and their computer implementation that would establish the dependence of pH (acidity of the environment) on the volume and structure of raw materials for daily loading, as well as on the operating parameters of temperature and humidity based on the interval analysis of experimental data obtained during BGP research of a given type. In the process of research, based on the developed interval models, it was established that this indicator depends on the volume and structure of raw materials, as well as on the temperature and humidity of the substrate in the bioreactor. To build this mathematical model, it is proposed to use the method of interval data analysis and the method of identification of model parameters based on multidimensional optimization. The results of experimental studies for a specific type of biogas plant are given, and interval models with guaranteed prognostic properties that characterize the pH of the environment depending on the specific type of bio-raw material of solid and liquid fractions, temperature, and humidity are obtained. Based on the use of different types of raw materials, the developed models, based on experimental data, describe different configurations of the structure and volumes of raw materials for daily loading. The obtained mathematical models are an algebraic nonlinear equation that can be applied to control the level of pH of the environment in the bioreactor by determining the optimal volumes of raw materials of each type during the loading period depending on the temperature and humidity of the substrate in the bioreactor.
Fluid-Structure Numerical Study of an In-Pipe Axial Turbine with Circular Blades
Authors: Monsalve-Cifuentes Oscar D. , Vélez-García Sebastián , Sanín-Villa Daniel , Revuelta-Acosta Josept David
Published: July 2024

Hydraulic turbines have become indispensable for harnessing renewable energy sources, particularly in-pipe hydraulic turbine technology, which leverages excess energy within pipeline systems like drinking water distribution pipes to produce electrical power. Among these turbines, the propeller-type axial turbine with circular blades stands out for its efficiency. However, there is a notable lack of literature on fluid dynamics and structural behavior under various operational conditions. This study introduces a comprehensive methodology to numerically investigate the hydraulic and structural responses of turbines designed for in-pipe installation. The methodology encompasses the design of circular blades, followed by parametric studies on fluid dynamics and structural analysis. The circular blade’s performance was evaluated across different materials, incorporating static, modal, and harmonic response analyses. Results showed that the circular blade achieved a peak hydraulic efficiency of 75.5% at a flow rate of 10 l/s, generating 1.86 m of head pressure drop and 138 W of mechanical power. Structurally, it demonstrated a safety factor exceeding 1 across the entire hydraulic range without encountering resonance or fatigue issues. This research and its methodology significantly contribute to advancing the understanding of designing and assessing the fluid dynamic behavior and structural integrity of circular blades in axial propeller-type turbines for in-pipe installations, serving as a valuable resource for future studies in similar domains.
An Automated Cropland Burned-Area Detection Algorithm Based on Landsat Time Series Coupled with Optimized Outliers and Thresholds
Authors: Zhang Sumei , Li Huijuan , Zhao Hongmei
Published: July 2024

Given the increasingly severe global fires, the accurate detection of small and fragmented cropland fires has been a significant challenge. The use of medium-resolution satellite data can enhance detection accuracy; however, key challenges in this approach include accurately capturing the annual and interannual variations of burning characteristics and identifying outliers within the time series of these changes. In this study, we focus on a typical crop-straw burning area in Henan Province, located on the North China Plain. We develop an automated burned-area detection algorithm based on near-infrared and short-wave infrared data from Landsat 5 imagery. Our method integrates time-series outlier analysis using filtering and automatic iterative algorithms to determine the optimal threshold for detecting burned areas. Our results demonstrate the effectiveness of using preceding time-series and seasonal time-series analysis to differentiate fire-related changes from seasonal and non-seasonal influences on vegetation. Optimal threshold validation results reveal that the automatic threshold method is efficient and feasible with an overall accuracy exceeding 93%. The resulting burned-area map achieves a total accuracy of 93.25%, far surpassing the 76.5% detection accuracy of the MCD64A1 fire product, thereby highlighting the efficacy of our algorithm. In conclusion, our algorithm is suitable for detecting burned areas in large-scale farmland settings and provides valuable information for the development of future detection algorithms.
A Delphi–Fuzzy Delphi Study on SDGs 9 and 12 after COVID-19: Case Study in Brazil
Authors: Sousa Isabela Caroline de , Sigahi Tiago F. A. C. , Rampasso Izabela Simon , Moraes Gustavo Hermínio Salati Marcondes de , Leal Filho Walter , Eustachio João Henrique Paulino Pires , Anholon Rosley
Published: July 2024

The COVID-19 pandemic has affected all Sustainable Development Goals (SDGs), leading to setbacks in various Latin American countries. In Brazil, progress in technological development and the adoption of sustainable practices by organizations has been significantly hindered. Yet, there remains a limited understanding of the long-term impacts on the country’s development, and a structured national plan for recovery and resuming progress toward the SDGs is lacking. This paper aims to investigate the repercussions of COVID-19 on SDGs 9 (industry, innovation, and infrastructure) and 12 (sustainable consumption and production) in the context of a latecomer country such as Brazil. This study adopted the Delphi-based scenario and Fuzzy Delphi approach and involved the participation of 15 sustainability experts with extensive experience in the Brazilian industrial sector. The findings elucidate the long-term impacts of the pandemic on these SDGs, focusing on Brazil’s socioeconomic landscape and developmental challenges. The pandemic worsened pre-existing issues, hindering infrastructure modernization, technological investment, and sustainable practices. Insufficient research funding, industry modernization, and small business integration further impede progress. Additionally, the paper identifies implications for research, companies, and public policies, aiming to provide actionable insights for fostering sustainable development in the post-pandemic era.
Insights into the Injectivity and Propagation Behavior of Preformed Particle Gel (PPG) in a Low–Medium-Permeability Reservoir
Authors: He Hong , Tian Yuhang , Zhang Lianfeng , Li Hongsheng , Guo Yan , Liu Yu , Liu Yifei
Published: July 2024

Heterogeneous phase combined flooding (HPCF) has been a promising technology used for enhancing oil recovery in heterogeneous mature reservoirs. However, the injectivity and propagation behavior of preformed particle gel (PPG) in low–medium-permeability reservoir porous media is crucial for HPCF treatment in a low–medium-permeability reservoir. Thus, the injectivity and propagation behavior of preformed particle gel in a low–medium-permeability reservoir were systematically studied by conducting a series of sand pack flooding experiments. The matching factor (δ) was defined as the ratio of the average size of PPG particles to the mean size of pore throats and the pressure difference ratio (β) was proposed to characterize the injectivity and propagation ability of PPG. The results show that with the increase in particle size and the decrease in permeability, the resistance factor and residual resistance factor increase. With the increase in the matching factor, the resistance factor and residual resistance factor increase. The higher the resistance factor and residual resistance factor are, the worse the injectivity of particles is. By fitting the relationship curve, PPG injection and propagation standards were established: when the matching coefficient is less than 55 and β is less than 3.4, PPG can be injected; when the matching coefficient is 55–72 and β is 3.4–6.5, PPG injection is difficult; when the matching coefficient is greater than 72 and β is greater than 6.5, PPG cannot be injected Thus, the matching relationship between PPG particle size and reservoir permeability was obtained. This research will provide theoretical support for further EOR research and field application of heterogeneous phase combined flooding.
Comprehensive Biosafety Profile of Carbomer-Based Hydrogel Formulations Incorporating Phosphorus Derivatives
Authors: Zakzak Khaled , Semenescu Alexandra-Denisa , Moacă Elena-Alina , Predescu Iasmina , Drăghici George , Vlaia Lavinia , Vlaia Vicenţiu , Borcan Florin , Dehelean Cristina-Adriana
Published: July 2024

Determining the safety of a newly developed experimental product is a crucial condition for its medical use, especially for clinical trials. In this regard, four hydrogel-type formulations were manufactured, all of which were based on carbomer (Blank-CP940) and encapsulated with caffeine (CAF-CP940), phosphorus derivatives (phenyl phosphinic (CAF-S1-CP940) and 2-carboxyethyl phenyl phosphinic acids (CAF-S2-CP940)). The main aim of this research was to provide a comprehensive outline of the biosafety profile of the above-mentioned hydrogels. The complex in vitro screening (cell viability, cytotoxicity, morphological changes in response to exposure, and changes in nuclei morphology) on two types of healthy skin cell lines (HaCaT—human keratinocytes and JB6 Cl 41-5a—murine epidermal cells) exhibited a good biosafety profile when both cell lines were treated for 24 h with 150 μg/mL of each hydrogel. A comprehensive analysis of the hydrogel’s impact on the genetic profile of HaCaT cells sustains the in vitro experiments. The biosafety profile was completed with the in vivo and in ovo assays. The outcome revealed that the developed hydrogels exerted good biocompatibility after topical application on BALB/c nude mice’s skin. It also revealed a lack of toxicity after exposure to the hen’s chicken embryo. Further investigations are needed, regarding the in vitro and in vivo therapeutic efficacy and safety for long-term use and potential clinical translatability.
Heavens of Knowledge: The Order of Sciences in Dante’s Convivio
Authors: Pegoretti Anna
Published: July 2024

The essay focuses on Dante’s divisio scientiae presented in the second book of his Convivio. As a first step, it offers a fresh reading of Dante’s description of knowledge and the cosmos, emphasizing its deeply visual nature. The article then presents an overview of the Medieval tradition of the divisio scientiae, culminating with divisions that are geographically and chronologically close to Dante’s Florence. In relation to Brunetto Latini’s Rettorica and Tresor, new evidence is provided to elucidate his peculiar division of logic. Ultimately, Dante’s divisio and its objectives are reassessed in light of their historical background, underscoring their cosmological and totalizing scope. Adopting an interdisciplinary approach, the findings consider cognitive practices, such as diagrams, and examine a wide array of sources within their historical and institutional context, highlighting their transmission and dissemination.
Assessment of the Severity of COVID-19 on the Basis of Examination and Laboratory Diagnostics in Relation to Computed Tomography Imagery of Patients Hospitalised Due to COVID-19—Single-Centre Study
Authors: Ilczak Tomasz , Skoczynski Szymon , Oclon Ewa , Kucharski Mirosław , Strejczyk Tomasz , Jagosz Marta , Jedynak Antonina , Wita Michał , Ćwiertnia Michał , Jędrzejek Marek , Dutka Mieczysław , Waksmańska Wioletta , Bobiński Rafał , Pakuła Roch , Kawecki Marek , Kukla Paweł , Białka Szymon
Published: July 2024

From the moment the SARS-CoV-2 virus was identified in December 2019, the COVID-19 disease spread around the world, causing an increase in hospitalisations and deaths. From the beginning of the pandemic, scientists tried to determine the major cause that led to patient deaths. In this paper, the background to creating a research model was diagnostic problems related to early assessment of the degree of damage to the lungs in patients with COVID-19. The study group comprised patients hospitalised in one of the temporary COVID hospitals. Patients admitted to the hospital had confirmed infection with SARS-CoV-2. At the moment of admittance, arterial blood was taken and the relevant parameters noted. The results of physical examinations, the use of oxygen therapy and later test results were compared with the condition of the patients in later computed tomography images and descriptions. The point of reference for determining the severity of the patient’s condition in the computer imagery was set for a mild condition as consisting of a percentage of total lung parenchyma surface area affected no greater than 30%, an average condition of between 30% and 70%, and a severe condition as greater than 70% of the lung parenchyma surface area affected. Patients in a mild clinical condition most frequently had mild lung damage on the CT image, similarly to patients in an average clinical condition. Patients in a serious clinical condition most often had average levels of damage on the CT image. On the basis of the collected data, it can be said that at the moment of admittance, BNP, PE and HCO3− levels, selected due to the form of lung damage, on computed tomography differed from one another in a statistically significant manner (p < 0.05). Patients can qualify for an appropriate group according to the severity of COVID-19 on the basis of a physical examination and applied oxygen therapy. Patients can qualify for an appropriate group according to the severity of COVID-19 on the basis of BNP, HCO3 and BE parameters obtained from arterial blood.
Evaluating the Cold Tolerance of Stenotaphrum Trin Plants by Integrating Their Performance at Both Fall Dormancy and Spring Green-Up
Authors: Qu Jia , Hao Dong-Li , Zhou Jin-Yan , Chen Jing-Bo , Sun Dao-Jin , Liu Jian-Xiu , Zong Jun-Qin , Wang Zhi-Yong
Published: July 2024

Owing to the poor cold tolerance of Stenotaphrum Trin and the urgent need for shade-tolerant grass species in temperate regions of East China, this study evaluated the cold tolerance of 55 Stenotaphrum accessions, aiming to provide shade-tolerant materials for temperate regions. A fine cold-tolerant turfgrass should have both the advantages of delayed fall dormancy and early spring green-up. However, previous research on the cold resistance of turfgrass has mainly focused on the performance of the spring green-up, with less attention paid to the fall dormancy, which has affected the ornamental and application value of turfgrass. This study first dynamically investigated the leaf colour of each accession during the fall dormancy and the coverage during the spring green-up and evaluated the cold resistance of the accession through membership functions and cluster analysis. Significant differences in the cold resistance were found with the assignment of breeding lines to four categories. The weak correlation (R2 = 0.1682) between leaf colour during the fall dormancy and coverage during the spring green-up indicates that using the performance of a single period to represent the cold resistance of accessions is not appropriate. To test whether using the laboratory-based LT50 and stolon regrowth rating analysis can replace the above-improved method, we conducted a related analysis and found that the fit between these two methods is very poor. This phenomenon is attributed to the poor correlation between the laboratory-based parameters and the pot-investigated data. Therefore, this study presents a cold resistance evaluation method for Stenotaphrum that integrates performance in both the fall dormancy and spring green-up periods. This improved evaluation method cannot be simplified by the growth performance of a single period or replaced by using laboratory-based LT50 and stolon regrowth tests. With the help of this improved method, several excellent cold tolerance accessions (ST003, S13, and S12) were identified for temperate regions of East China.
Hydrogels Based on Proteins Cross-Linked with Carbonyl Derivatives of Polysaccharides, with Biomedical Applications
Authors: Mahmoudi Chahrazed , Tahraoui Douma Naïma , Mahmoudi Hacene , Iurciuc (Tincu) Camelia Elena , Popa Marcel
Published: July 2024

Adding carbonyl groups into the hydrogel matrix improves the stability and biocompatibility of the hydrogels, making them suitable for different biomedical applications. In this review article, we will discuss the use of hydrogels based on polysaccharides modified by oxidation, with particular attention paid to the introduction of carbonyl groups. These hydrogels have been developed for several applications in tissue engineering, drug delivery, and wound healing. The review article discusses the mechanism by which oxidized polysaccharides can introduce carbonyl groups, leading to the development of hydrogels through cross-linking with proteins. These hydrogels have tunable mechanical properties and improved biocompatibility. Hydrogels have dynamic properties that make them promising biomaterials for various biomedical applications. This paper comprehensively analyzes hydrogels based on cross-linked proteins with carbonyl groups derived from oxidized polysaccharides, including microparticles, nanoparticles, and films. The applications of these hydrogels in tissue engineering, drug delivery, and wound healing are also discussed.
Advancements in Biomaterials for Bioengineering and Biotechnology
Authors: Tsouknidas Alexander
Published: July 2024

Biomaterials, whether of biological or synthetic origin, have risen to the forefront of modern medical innovation since the early 2000s, transcending their traditional roles in orthopedic and dental applications, to encompass drug delivery systems, implantable biosensors, and templates for cellular growth and tissue regeneration [...]
Genus Sambucus: Exploring Its Potential as a Functional Food Ingredient with Neuroprotective Properties Mediated by Antioxidant and Anti-Inflammatory Mechanisms
Authors: Merecz-Sadowska Anna , Sitarek Przemysław , Zajdel Karolina , Sztandera Wiktoria , Zajdel Radosław
Published: July 2024

The genus Sambucus, mainly Sambucus nigra, has emerged as a valuable source of bioactive compounds with potential neuroprotective properties. This review explores the antioxidant, anti-inflammatory, and neuroregenerative effects of Sambucus-derived compounds and their implications for brain health and cognitive function. In vitro studies have demonstrated the ability of Sambucus extracts to mitigate oxidative stress, modulate inflammatory responses, and promote neural stem cell proliferation and differentiation. In vivo studies using animal models of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, have shown that Sambucus compounds can improve cognitive function, motor performance, and neuronal survival while attenuating neuroinflammation and oxidative damage. The neuroprotective effects of Sambucus are primarily attributed to its rich content of polyphenols, particularly anthocyanins, which exert their benefits through multiple mechanisms, including the modulation of signaling pathways involved in inflammation, apoptosis, mitochondrial function, and oxidative stress. Furthermore, the potential of Sambucus as a functional food ingredient is discussed, highlighting its application in various food products and the challenges associated with the stability and bioavailability of its bioactive compounds. This review provides a comprehensive overview of the current state of research on the neuroprotective potential of Sambucus and its derivatives, offering valuable insights for the development of dietary strategies to promote brain health and prevent age-related cognitive decline.
Assessment of Pyrogenic Response of Medical Devices and Biomaterials by the Monocyte Activation Test (MAT): A Systematic Review
Authors: Gimenes Izabela , Spoladore Janaína , Paranhos Bruno Andrade , Romasco Tea , Di Pietro Natalia , Piattelli Adriano , Mourão Carlos Fernando , Gomes Alves Gutemberg
Published: July 2024

Pyrogens are fever-inducing substances routinely investigated in health products through tests such as the Rabbit Pyrogen Test (RPT), the Limulus Amebocyte Lysate (LAL), and the Monocyte Activation Test (MAT). However, the applications of the MAT for medical devices and biomaterials remain limited. This work aimed to overview the studies evaluating the pyrogenicity of medical devices and biomaterials using the MAT, highlighting its successes and potential challenges. An electronic search was performed by December 2023 in PubMed, Scopus, and Web of Science, identifying 321 records which resulted in ten selected studies. Data were extracted detailing the tested materials, MAT variants, interferences, and comparisons between methods. Methodological quality was assessed using the ToxRTool, and the results were synthesized descriptively. The selected studies investigated various materials, including polymers, metals, and natural compounds, employing the different biological matrices of the MAT. Results showed the MAT’s versatility, with successful detection of pyrogens in most materials tested, though variability in sensitivity was noted based on the material and testing conditions. Challenges remain in optimizing protocols for different material properties, such as determining the best methods for direct contact versus eluate testing and addressing the incubation conditions. In conclusion, the MAT demonstrates significant potential as a pyrogen detection method for medical devices and biomaterials. However, continued research is essential to address existing gaps, optimize protocols, and validate the test across a broader range of materials.
Debates Surrounding the Use of Antithrombotic Therapy in Hemophilic Patients with Cardiovascular Disease: Best Strategies to Minimize Severe Bleeding Risk
Authors: Badulescu Oana-Viola , Scripcariu Dragos Viorel , Badescu Minerva Codruta , Ciocoiu Manuela , Vladeanu Maria Cristina , Plesoianu Carmen Elena , Bojan Andrei , Iliescu-Halitchi Dan , Tudor Razvan , Huzum Bogdan , Bararu Bojan Iris
Published: July 2024

Navigating through antithrombotic therapy in patients with both hemophilia and cardiovascular pathology presents a complex scenario with inherent challenges and opportunities. The presence of hemophilia, characterized by impaired blood clotting, adds a layer of complexity to the management of cardiovascular conditions requiring antiplatelet therapy and anticoagulation. Striking a delicate balance between the necessity for antithrombotic treatment to prevent cardiovascular events and the heightened risk of severe bleeding in individuals with hemophilia demands a nuanced and carefully considered approach. The challenges revolve around identifying an optimal therapeutic strategy that effectively mitigates cardiovascular risks without exacerbating bleeding tendencies. In hemophilic patients with cardiovascular disease, the decision to use antiplatelet therapy requires careful consideration of the individual’s bleeding risk profile, considering factors such as the severity of hemophilia, history of bleeding episodes, and concurrent medications. The goal is to provide effective antithrombotic treatment while minimizing the potential for excessive bleeding complications. Conventional anticoagulants like warfarin pose difficulties due to their potential to increase the risk of bleeding. On the other hand, emerging options like novel direct oral anticoagulants (DOACs) present an opportunity, offering predictable pharmacokinetics and user-friendly administration. However, a comprehensive exploration of their safety and efficacy in hemophilic patients is imperative. Achieving the right equilibrium between preventing cardiovascular events and minimizing bleeding risk is pivotal in selecting the most effective therapeutic option for individuals with hemophilia and cardiovascular pathology. A multidisciplinary approach, integrating the expertise of hematologists and cardiologists, becomes essential to customize treatments and address the intricacies of this medical challenge.
Cilostazol Ameliorates Motor Dysfunction and Schwann Cell Impairment in Streptozotocin-Induced Diabetic Rats
Authors: Chang Lin-Li , Wu Yu-Ming , Wang Hung-Chen , Tseng Kuang-Yi , Wang Yi-Hsuan , Lu Yen-Mou , Cheng Kuang-I
Published: July 2024

This study investigated the effects of cilostazol on motor dysfunction, spinal motor neuron abnormalities, and schwannopathy in rats with diabetes. Diabetes mellitus (DM) was induced in rats via femoral intravenous streptozotocin (STZ) injection (60 mg/kg). After successful DM induction, cilostazol was administered on day 15 via oral gavage (100 mg/kg/day) for 6 weeks until sacrifice. Behavioral assays, including motor function, were performed weekly. The sciatic nerve, L5 spinal cord, and spinal ventral root were collected to evaluate the expression of the glial fibrillary acidic protein (GFAP), myelin protein zero (P0), and choline acetyltransferase (ChAT) by immunofluorescence and Western blotting. DM rats displayed decreased running speeds, running distances, and toe spread but increased foot pressure. In addition, loss of non-myelinating Schwann cells and myelin sheaths was observed in the sciatic nerve and L5 spinal ventral root. Reduced numbers of motor neurons were also found in the L5 spinal ventral horn. Cilostazol administration significantly potentiated running speed and distance; increased hind paw toe spread; and decreased foot pressure. In the sciatic nerve and L5 spinal ventral root, cilostazol treatment significantly improved non-myelinated Schwann cells and increased myelin mass. ChAT expression in motor neurons in the spinal ventral horn was improved, but not significantly. Cilostazol administration may protect sensorimotor function in diabetic rats.
Adipose Tissue Dysfunction Related to Climate Change and Air Pollution: Understanding the Metabolic Consequences
Authors: Stojchevski Radoslav , Chandrasekaran Preethi , Hadzi-Petrushev Nikola , Mladenov Mitko , Avtanski Dimiter
Published: July 2024

Obesity, a global pandemic, poses a major threat to healthcare systems worldwide. Adipose tissue, the energy-storing organ during excessive energy intake, functions as a thermoregulator, interacting with other tissues to regulate systemic metabolism. Specifically, brown adipose tissue (BAT) is positively associated with an increased resistance to obesity, due to its thermogenic function in the presence of uncoupled protein 1 (UCP1). Recently, studies on climate change and the influence of environmental pollutants on energy homeostasis and obesity have drawn increasing attention. The reciprocal relationship between increasing adiposity and increasing temperatures results in reduced adaptive thermogenesis, decreased physical activity, and increased carbon footprint production. In addition, the impact of climate change makes obese individuals more prone to developing type 2 diabetes mellitus (T2DM). An impaired response to heat stress, compromised vasodilation, and sweating increase the risk of diabetes-related comorbidities. This comprehensive review provides information about the effects of climate change on obesity and adipose tissue, the risk of T2DM development, and insights into the environmental pollutants causing adipose tissue dysfunction and obesity. The effects of altered dietary patterns on adiposity and adaptation strategies to mitigate the detrimental effects of climate change are also discussed.
How Histone Acetyltransferases Shape Plant Photomorphogenesis and UV Response
Authors: Boycheva Irina , Bonchev Georgi , Manova Vasilissa , Stoilov Lubomir , Vassileva Valya
Published: July 2024

Higher plants have developed complex mechanisms to adapt to fluctuating environmental conditions with light playing a vital role in photosynthesis and influencing various developmental processes, including photomorphogenesis. Exposure to ultraviolet (UV) radiation can cause cellular damage, necessitating effective DNA repair mechanisms. Histone acetyltransferases (HATs) play a crucial role in regulating chromatin structure and gene expression, thereby contributing to the repair mechanisms. HATs facilitate chromatin relaxation, enabling transcriptional activation necessary for plant development and stress responses. The intricate relationship between HATs, light signaling pathways and chromatin dynamics has been increasingly understood, providing valuable insights into plant adaptability. This review explores the role of HATs in plant photomorphogenesis, chromatin remodeling and gene regulation, highlighting the importance of chromatin modifications in plant responses to light and various stressors. It emphasizes the need for further research on individual HAT family members and their interactions with other epigenetic factors. Advanced genomic approaches and genome-editing technologies offer promising avenues for enhancing crop resilience and productivity through targeted manipulation of HAT activities. Understanding these mechanisms is essential for developing strategies to improve plant growth and stress tolerance, contributing to sustainable agriculture in the face of a changing climate.
Oral Exposure to Low Concentration of Fumonisin B2, but Not Fumonisin B1, Significantly Exacerbates the Pathophysiology of Imiquimod-Induced Psoriasis in Mice
Authors: Ando Mana , Yamaguchi Hiroki , Iwashita Naoki , Takagi Yoshiichi , Yoshinari Tomoya , Fukuyama Tomoki
Published: July 2024

This study aimed to determine whether oral fumonisin exposure contributes to the development of psoriasis. Oral administration of fumonisin B1 (FB1, 0.1 mg/kg) or fumonisin B2 (FB2, 0.1 mg/kg) was conducted for 10 days, in addition to the induction of psoriatic symptoms through topical application of 5% imiquimod cream from day 6 to day 10 (5 days) in female BALB/c mice. The results demonstrated that oral administration of FB2 significantly exacerbated psoriatic symptoms, including skin thickness, itching behavior, transepidermal water loss, immune cell infiltration in the dermis, and proinflammatory cytokine production. However, no changes were observed following exposure to FB1. Our results confirm that oral exposure to FB2 adversely affects the pathogenesis of psoriasis by increasing skin thickness and impairing barrier function.
The Impact of Tobacco Smoking and Alcohol Consumption on the Development of Gastric Cancers
Authors: Hatta Waku , Koike Tomoyuki , Asano Naoki , Hatayama Yutaka , Ogata Yohei , Saito Masahiro , Jin Xiaoyi , Uno Kaname , Imatani Akira , Masamune Atsushi
Published: July 2024

Chronic infection of Helicobacter pylori is considered the principal cause of gastric cancers, but evidence has accumulated regarding the impact of tobacco smoking and alcohol consumption on the development of gastric cancers. Several possible mechanisms, including the activation of nicotinic acetylcholine receptors, have been proposed for smoking-induced gastric carcinogenesis. On the other hand, local acetaldehyde exposure and ethanol-induced mucosal inflammation have been proposed as the mechanisms involved in the development of gastric cancers in heavy alcohol drinkers. In addition, genetic polymorphisms are also considered to play a pivotal role in smoking-related and alcohol-related gastric carcinogenesis. In this review, we will discuss the molecular mechanisms involved in the development of gastric cancers in relation to tobacco smoking and alcohol consumption.
Inner Structure of the Lateral Geniculate Complex of Adult and Newborn Acomys cahirinus
Authors: Merkulyeva Natalia , Mikhalkin Aleksandr , Veshchitskii Aleksandr
Published: July 2024

Acomys cahirinus is a unique Rodentia species with several distinctive physiological traits, such as precocial development and remarkable regenerative abilities. These characteristics render A. cahirinus increasingly valuable for regenerative and developmental physiology studies. Despite this, the structure and postnatal development of the central nervous system in A. cahirinus have been inadequately explored, with only sporadic data available. This study is the first in a series of papers addressing these gaps. Our first objective was to characterize the structure of the main visual thalamic region, the lateral geniculate complex, using several neuronal markers (including Ca2+-binding proteins, glutamic acid decarboxylase enzyme, and non-phosphorylated domains of heavy-chain neurofilaments) to label populations of principal neurons and interneurons in adult and newborn A. cahirinus. As typically found in other rodents, we identified three subdivisions in the geniculate complex: the dorsal and ventral lateral geniculate nuclei (LGNd and LGNv) and the intergeniculate leaflet (IGL). Additionally, we characterized internal diversity in the LGN nuclei. The “shell” and “core” regions of the LGNd were identified using calretinin in adults and newborns. In adults, the inner and outer parts of the LGNv were identified using calbindin, calretinin, parvalbumin, GAD67, and SMI-32, whereas in newborns, calretinin and SMI-32 were employed for this purpose. Our findings revealed more pronounced developmental changes in LGNd compared to LGNv and IGL, suggesting that LGNd is less mature at birth and more influenced by visual experience.
Analysis of Carcinogenic Involvement of MicroRNA Pattern in Peripheral Non-Cancerous Tissues and Chronic Viral Liver Injury
Authors: Umezu Tomohiro , Mori Tomoya , Toyoda Hidenori , Kanekura Kohsuke , Tamori Akihiro , Ochiya Takahiro , Kuroda Masahiko , Akutsu Tatsuya , Murakami Yoshiki
Published: July 2024

Risk factors for hepatocarcinogenesis include chronic inflammation due to viral infection, liver fibrosis, and aging. In this study, we separated carcinogenic and non-carcinogenic cases due to hepatitis C virus (HCV) infection, aiming to comprehensively analyze miRNA expression in liver tissues by age, and identify factors that contribute to carcinogenesis. Total RNA was extracted from 360 chronic hepatitis C (CH), 43 HCV infected hepatocellular carcinoma (HCC), and surrounding non-tumor (SNT) tissues. MicroRNA (miRNA) expression patterns were analyzed using microarray. Using machine learning, we extracted characteristic miRNA expression patterns for each disease and age. There were no age-dependent changes in miRNA expression in the disease-specific comparisons; however, miRNA expression differed among the age groups of 50, 60, and 70 years of age between CH and SNT. The expression of miRNA was different between SNT and HCC only in patients in their 70s. Of the 55 miRNAs with significant differences in expression between CH and SNT, 34 miRNAs showed significant differences in expression even in the degree of liver fibrosis. The observation that miRNAs involved in hepatocarcinogenesis differ at different ages suggests that the mechanisms of carcinogenesis differ by age group as well. We also found that many miRNAs whose expression did not affect liver fibrosis were involved in carcinogenesis. These findings are expected to define biomarkers for detection of HCC at early stage, and develop novel therapeutic targets for HCC.
Semliki Forest Virus (SFV) Self-Amplifying RNA Delivered to J774A.1 Macrophage Lineage by Its Association with a Purified Recombinant SFV Capsid Protein
Authors: Gomes Roselane P. , Barbosa Flavia F. , Toledo Marcelo A. S. , Jorge Soraia A. C. , Astray Renato M.
Published: July 2024

The Semliki Forest virus capsid protein (C) is an RNA binding protein which exhibits both specific and unspecific affinities to single-strand nucleic acids. The putative use of the self-amplifying RNAs (saRNAs) of alphaviruses for biotechnological purpose is one of the main studied strategies concerning RNA-based therapies or immunization. In this work, a recombinant C protein from SFV was expressed and purified from bacteria and used to associate in vitro with a saRNA derived from SFV. Results showed that the purified form of C protein can associate with the saRNA even after high temperature treatment. The C protein was associated with a modified saRNA coding for the green fluorescent protein (GFP) and delivered to murine macrophage cells which expressed the GFP, showing that the saRNA was functional after being associated with the recombinant purified C protein.
A New Tailored Nanodroplet Carrier of Astaxanthin Can Improve Its Pharmacokinetic Profile and Antioxidant and Anti-Inflammatory Efficacies
Authors: Mishra Kumudesh , Khatib Nadin , Barasch Dinorah , Kumar Pradeep , Garti Sharon , Garti Nissim , Kakhlon Or
Published: July 2024

Astaxanthin (ATX) is a carotenoid nutraceutical with poor bioavailability due to its high lipophilicity. We tested a new tailored nanodroplet capable of solubilizing ATX in an oil-in-water micro-environment (LDS-ATX) for its capacity to improve the ATX pharmacokinetic profile and therapeutic efficacy. We used liquid chromatography tandem mass spectrometry (LC-MS/MS) to profile the pharmacokinetics of ATX and LDS-ATX, superoxide mutase (SOD) activity to determine their antioxidant capacity, protein carbonylation and lipid peroxidation to compare their basal and lipopolysaccharide (LPS)-induced oxidative damage, and ELISA-based detection of IL-2 and IFN-γ to determine their anti-inflammatory capacity. ATX and LDS-ATX corrected only LPS-induced SOD inhibition and oxidative damage. SOD activity was restored only by LDS-ATX in the liver and brain and by both ATX and LDS-ATX in muscle. While in the liver and muscle, LDS-ATX attenuated oxidative damage to proteins and lipids better than ATX; only oxidative damage to lipids was preferably corrected by LDS-ATX in the brain. IL-2 and IFN-γ pro-inflammatory response was corrected by LDS-ATX and not ATX in the liver and brain, but in muscle, the IL-2 response was not corrected and the IFN-γ response was mitigated by both. These results strongly suggest an organ-dependent improvement of ATX bioavailability and efficacy by the LDS-ATX nanoformulation.
Rv0687 a Putative Short-Chain Dehydrogenase Is Required for In Vitro and In Vivo Survival of Mycobacterium tuberculosis
Authors: Bhargavi Gunapati , Mallakuntla Mohan Krishna , Kale Deepa , Tiwari Sangeeta
Published: July 2024

Mycobacterium tuberculosis (Mtb), a successful human pathogen, resides in host sentinel cells and combats the stressful intracellular environment induced by reactive oxygen and nitrogen species during infection. Mtb employs several evasion mechanisms in the face of the host as a survival strategy, including detoxifying enzymes as short-chain dehydrogenases/reductases (SDRs) to withstand host-generated insults. In this study, using specialized transduction, we have generated a Rv0687 deletion mutant and its complemented strain and investigated the functional role of Rv0687, a member of SDRs family genes in Mtb pathogenesis. A wildtype (WT) and a mutant Mtb strain lacking Rv0687 (RvΔ0687) were tested for the in vitro stress response and in vivo survival in macrophages and mice models of infection. The study demonstrates that the deletion of Rv0687 elevated the sensitivity of Mtb to oxidative and nitrosative stress-inducing agents. Furthermore, the lack of Rv0687 compromised the survival of Mtb in primary bone marrow macrophages and led to an increase in the levels of the secreted proinflammatory cytokines TNF-α and MIP-1α. Interestingly, the growth of WT and RvΔ0687 was similar in the lungs of infected immunocompromised mice; however, a significant reduction in RvΔ0687 growth was observed in the spleen of immunocompromised Rag−/− mice at 4 weeks post-infection. Moreover, Rag−/− mice infected with RvΔ0687 survived longer compared to those infected with the WT Mtb strain. Additionally, we observed a significant reduction in the bacterial burden in the spleens and lungs of immunocompetent C57BL/6 mice infected with RvΔ0687 compared to those infected with complemented and WT Mtb strains. Collectively, this study reveals that Rv0687 plays a role in Mtb pathogenesis.
Cyclic Nitroxide 4-Methoxy-Tempo May Decrease Serum Amyloid A-Mediated Renal Fibrosis and Reorganise Collagen Networks in Aortic Plaque
Authors: Gao Antony , Xie Kangzhe , Gupta Sameesh , Ahmad Gulfam , Witting Paul K.
Published: July 2024

Acute-phase serum amyloid A (SAA) can disrupt vascular homeostasis and is elevated in subjects with diabetes, cardiovascular disease, and rheumatoid arthritis. Cyclic nitroxides (e.g., Tempo) are a class of piperidines that inhibit oxidative stress and inflammation. This study examined whether 4-methoxy-Tempo (4-MetT) inhibits SAA-mediated vascular and renal dysfunction. Acetylcholine-mediated vascular relaxation and aortic guanosine-3′,5′-cyclic monophosphate (cGMP) levels both diminished in the presence of SAA. 4-MetT dose-dependently restored vascular function with corresponding increases in cGMP. Next, male ApoE-deficient mice were administered a vehicle (control, 100 µL PBS) or recombinant SAA (100 µL, 120 µg/mL) ± 4-MetT (at 15 mg/kg body weight via i.p. injection) with the nitroxide administered before (prophylaxis) or after (therapeutic) SAA. Kidney and hearts were harvested at 4 or 16 weeks post SAA administration. Renal inflammation increased 4 weeks after SAA treatment, as judged by the upregulation of IFN-γ and concomitant increases in iNOS, p38MAPK, and matrix metalloproteinase (MMP) activities and increased renal fibrosis (Picrosirius red staining) in the same kidneys. Aortic root lesions assessed at 16 weeks revealed that SAA enhanced lesion size (vs. control; p < 0.05), with plaque presenting with a diffuse fibrous cap (compared to the corresponding aortic root from control and 4-MetT groups). The extent of renal dysfunction and aortic lesion size was largely unchanged in 4-MetT-supplemented mice, although renal fibrosis diminished at 16 weeks, and aortic lesions presented with redistributed collagen networks. These outcomes indicate that SAA stimulates renal dysfunction through promoting the IFN-γ–iNOS–p38MAPK axis, manifesting as renal damage and enhanced atherosclerotic lesions, while supplementation with 4-MetT only affected some of these pathological changes.
Joint Inflammation Correlates with Joint GPR30 Expression in Males and Hippocampal GPR30 Expression in Females in a Rat Model of Rheumatoid Arthritis
Authors: Grubić Kezele Tanja , Omrčen Hrvoje , Batičić Lara , Šućurović Sandra , Zoričić Cvek Sanja
Published: July 2024

It is not entirely clear how the interaction between joint inflammation and the central nervous system (CNS) response in rheumatoid arthritis (RA) works, and what pathophysiology underlies the sex differences in coexisting neuropsychiatric comorbidities. It is known that estrogen hormones reduce inflammation in RA and that this occurs mainly via the stimulation of G protein-coupled receptor-30 (GPR30), also known as G protein-coupled estrogen receptor (GPER) 1. However, changes in GPR30 expression and sex differences induced by local and systemic inflammation in RA are not yet known. Our aim was to reveal sex differences in the expression and association of joint GPR30 with local and systemic inflammation, clinical course and furthermore with hippocampal GPR30 expression during pristane-induced arthritis (PIA) in Dark Agouti (DA) rats, an animal model of RA. Furthermore, we demonstrated sex-specific differences in the association between joint and systemic inflammation and hippocampal microglia during PIA. Our results suggest sex-specific differences not only in the clinical course and serum levels of pro-inflammatory cytokines but also in the expression of GPR30. Female rats show greater synovial inflammation and greater damage to the articular cartilage compared to males during PIA attack. Male rats express higher levels of synovial and cartilaginous GPR30 than females during PIA, which correlates with a less severe clinical course. The correlation between synovial and cartilaginous GPR30 and joint inflammation scores (Krenn and Mankin) in male rats suggests that the more severe the joint inflammation, the higher the GPR30 expression. At the same time, there is no particular upregulation of hippocampal GPR30 in males. On the other hand, female rats express higher levels of neuroprotective GPR30 in the hippocampus than male rats at the basic level and during PIA attack. In addition, females have a higher number of Iba-1+ cells in the hippocampus during PIA attack that strongly correlates with the clinical score, serum levels of IL-17A, and Krenn and Mankin scores. These results suggest that male rats are better protected from inflammation in the joints and female rats are better protected from the inflammation in the hippocampus during a PIA attack, independently of microglia proliferation. However, in the remission phase, synovial GPR30 expression suddenly increases in female rats, as does hippocampal GPR30 expression in males. Further experiments with a longer remission period are needed to investigate the molecular background of these sex differences, as well as microglia phenotype profiling.