bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychometrics of Persian Version of the Ageism Survey Among an Iranian Older Adult Population During COVID-19 Pandemic
Authors: Sharif Nia Hamid , She Long , Rasiah Ratneswary , Khoshnavay Fomani Fatemeh , Kaveh Omolhoda , Pahlevan Sharif Saeed , Hosseini Lida
Published: November 2021

Background: Studies have revealed an increase in discrimination, neglect, and abuse among the older adult population during this period. This study assessed the validity and reliability of the Persian version of the ageism survey instrument tested on a sample of the Iranian older adult population during coronavirus disease (COVID-19) pandemic. An important move in counteracting ageism is to classify the ageism scale comprehensively by employing adequate psychometrics.

Methods: The Persian version of the ageism scale was developed using a two-step procedure. The first step involved translating and revising the original scale to develop a Persian version of the ageism scale. The second step involved assessing the psychometric features of the newly adapted scale using construct validity through exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) and thereafter assessing the reliability through the average inter-item correlation (AIC), Cronbach's alpha. The sample consisted of 400 older adults (age 65 and older), who were recruited through online data collection, with samples for EFA and CFA randomly selected from the total samples.

Results: The Persian version of the ageism survey has three factors: age-related deprivation with five items, dignity with three items, and employment with three items; all of which explained 57.02% of the total variance. The outcome of the EFA was verified by the CFA, with internal consistency reliability being excellent (Cronbach's alpha was 0.725, 0.698, and 0.708 for the three factors).

Conclusion: This study specifically offers a restructured three factors Persian version of the ageism survey for Iranian older adults with acceptable construct validity and reliability.
A Quasi-Experimental Study of Medicaid Expansion and Urban Mortality in the American Northeast
Authors: Ayubcha Cyrus , Pouladvand Pedram , Ayubcha Soussan
Published: November 2021

Objectives: To investigate the association of state-level Medicaid expansion and non-elderly mortality rates from 1999 to 2018 in Northeastern urban settings.

Methods: This quasi-experimental study utilized a synthetic control method to assess the association of Medicaid expansion on non-elderly urban mortality rates [1999–2018]. Counties encompassing the largest cities in the Northeastern Megalopolis (Washington D.C., Baltimore, Philadelphia, New York City, and Boston) were selected as treatment units (n = 5 cities, 3,543,302 individuals in 2018). Cities in states without Medicaid expansion were utilized as control units (n = 17 cities, 12,713,768 individuals in 2018).

Results: Across all cities, there was a significant reduction in the neoplasm (Population-Adjusted Average Treatment Effect = −1.37 [95% CI −2.73, −0.42]) and all-cause (Population-Adjusted Average Treatment Effect = −2.57 [95%CI −8.46, −0.58]) mortality rate. Washington D.C. encountered the largest reductions in mortality (Average Treatment Effect on All-Cause Medical Mortality = −5.40 monthly deaths per 100,000 individuals [95% CI −12.50, −3.34], −18.84% [95% CI −43.64%, −11.67%] reduction, p = < 0.001; Average Treatment Effect on Neoplasm Mortality = −1.95 monthly deaths per 100,000 individuals [95% CI −3.04, −0.98], −21.88% [95% CI −34.10%, −10.99%] reduction, p = 0.002). Reductions in all-cause medical mortality and neoplasm mortality rates were similarly observed in other cities.

Conclusion: Significant reductions in urban mortality rates were associated with Medicaid expansion. Our study suggests that Medicaid expansion saved lives in the observed urban settings.
Knowledge, Attitude, and Practice Toward COVID-19 Among Sherubtse College Students in Bhutan: A Web-Based Cross-Sectional Study
Authors: Dorji Thinley , Wangmo Karma , Yezer  , Wangchuk Tashi , Tshokey  , Wangdi Kinley
Published: November 2021

Bhutan has reopened schools and colleges after an initial closure to contain coronavirus disease 2019 (COVID-19) transmission. However, the risk of transmissions is higher in the schools and colleges due to crowding. Therefore, this study aimed to assess the level of knowledge, attitude, and practice (KAP) toward COVID-19 among the students of Sherubtse College in Bhutan. A cross-sectional study using a questionnaire was conducted in September 2020 among the students of Sherubtse College, Bhutan. The questionnaire was made in the Google Forms and administered through a social forum WeChat app. The KAP scores were calculated that include mean scores. The association between the KAP was assessed using the Pearson's correlation coefficient. A total of 613 students participated in the survey. The majority of the participants (57%) were female and 56% were from the third year. The mean knowledge score was 10.7 (SD = 1.7; range 0–14), mean attitude score of 3.67 (SD = 1.0; range: 0–5), and mean practice score of 5.19 (SD = range: 0–6). A majority of the students had good knowledge (98%) and practice (93.5%) scores, and a positive attitude (86.6%) toward COVID-19. A positive but weak correlation between good knowledge and practice (r = 0.1, p = 0.0126) was observed. Having a positive attitude led to practicing appropriately most of the time (r = 0.1866, p < 0.001). The students had good KAP scores and followed the COVID-19 prevention protocols advocated by the government. Good knowledge and a positive attitude were translated into good practice. Therefore, the education campaign of the Bhutan government seems to be effective in the students.
Promoting Navigation Health Literacy at the Intersection of Schools and Communities. Development of the Game-Based Intervention Nebolus
Authors: Dadaczynski Kevin , Krah Verena , Frank Demian , Zügel-Hintz Elisabeth , Pöhlmann Fabrice
Published: November 2021

Emerging empirical evidence indicates a limited health literacy for a substantial proportion of children and adolescents. Although it is generally agreed upon promoting health literacy as early as possible in the lifespan, there is a lack of interventions addressing children and adolescents and their primary living environments. This article describes the development of Nebolus, a game-based intervention aiming to promote navigation health literacy at the intersection of schools and communities. Its intervention foundation lies in a socio-ecological understanding of health as well as in the Entertainment Education approach. Following an extensive literature search on health-related location-based games, a co-creation process was initiated that involved adolescents, community stakeholders, and design/IT professionals in all phases of the intervention development. The final Nebolus intervention includes three core activities: (1) a Nebolus rally app for adolescents aged 12 to 16 years, (2) an online planning tool allowing local health service providers/professionals to set up own Nebolus rallies, and (3) accompanying teaching material on health literacy in the school setting to be used before and after the Nebolus rallies. This article provides an overview of the intervention layout and discusses strengths and challenges of its development and implementation.
Animal Emotions—Do Animals Feel as We Do?
Authors: Krause Annika , Nawroth Christian
Published: November 2021

Emotions are an essential part of how we experience our world. Humans can express emotions by telling others how we feel—but what about animals? How can we tell whether they experience emotions and, if they do, which ones? When we think about the animals under human care, it is not only scientifically interesting but also ethically important to understand how these animals experience their worlds. Over the last 20 years, researchers have made considerable progress by identifying ways to assess emotions in animals. For example, researchers can look at the facial expressions of animals, record their vocalisations, or measure body processes such as changes in the heartbeat or hormone concentrations in the blood. This information can tell us more about how animals feel, why and how emotions have evolved, and what we, as humans, share with animals in our emotional experience of the world around us.
Developments in Ureteral Stent Technology
Authors: Lee Justin , Katz Matthew , Shah Ojas
Published: November 2021

Ureteral stents have been utilized for decades in maintaining ureteral patency, most commonly after ureteroscopy in the treatment of urolithiasis. Since their initial development, ureteral stents have had many technological advances that have allowed for better patient outcomes with improvements in comfort, durability, patency, encrustation resistance, biocompatibility, ease of insertion, migration, and biofilm development. Several new ureteral stents enter the market every year, each with their own touted benefits. It is essential to understand the different advantages for each ureteral stent to provide the best available care to patients when possible. The purpose of this review is to give a brief history of ureteral stent development and summarize the recent developments in ureteral stent designs. We aim to review the data supporting the clinical advantages of the latest ureteral stents available for use by urologists.
Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
Authors: Xian Jinwen , Zhao Pengpeng , Wang Ning , Wang Weiye , Zhang Yanyan , Meng Jimeng , Ma Xun , Wang Zhengrong , Bo Xinwen
Published: November 2021

Cystic echinococcosis (CE) is a cosmopolitan zoonosis caused by the larval stage ofEchinococcus granulosus, which affects humans and a wide range of mammalian intermediate hosts. Parasite tetraspanin proteins are crucial for host-parasite interactions, and therefore they may be useful for vaccine development or disease diagnosis. In the present study, the major antigen coding sequence of tetraspanin 11 (Eg-TSP11) fromE. granulosuswas determined. The results of immunolocalization showed that Eg-TSP11 was mainly located in the tegument of adult worms and protoscoleces. Western blotting analysis showed that the serum from dogs injected with recombinant Eg-TSP11 (rEg-TSP11) could recognize Eg-TSP11 among natural protoscolex proteins. Moreover, the serum from dogs withE. granulosusinfection also recognized rEg-TSP11. Serum indirect enzyme-linked immunosorbent assays demonstrated that IgG levels gradually increased after the first immunization with rEg-TSP11 compared with those in the control group. Furthermore, the serum levels of interleukin 4, interleukin 5, and interferon gamma were significantly altered in the rEg-TSP11 group. Importantly, we found that vaccination with rEg-TSP11 significantly decreased worm burden and inhibited segment development in a dog model ofE. granulosusinfection. Based on these findings, we speculated that rEg-TSP11 might be a potential candidate vaccine antigen againstE. granulosusinfection in dogs.
Precision Nutrient Rates and Placement in Conservation Maize-Wheat System: Effects on Crop Productivity, Profitability, Nutrient-Use Efficiency, and Environmental Footprints
Authors: Jat Raj K. , Bijarniya Deepak , Kakraliya Suresh K. , Sapkota Tek B. , Kakraliya Manish , Jat Mangi L.
Published: November 2021

Intensive tillage-based production systems coupled with inefficient fertilizer management practices have led to increased production costs, sub-optimal productivity, and significant environmental externalities. Conservation agriculture (CA) is being increasingly advocated as a management strategy to overcome these issues but precision nutrient management under the CA-based maize-wheat system is rarely studied. Two year’s (2014–2015 and 2015–2016) research was conducted at the research farm of BISA, Pusa, Bihar, India to develop precision nutrient management practices for CA-based management in the maize-wheat system. Seven treatment combinations involving (i) tillage (conventional tillage; CT & permanent beds; PB) and (ii) nutrient management rates, application methods (farmers’ fertilizer practices; FFP, state recommended dose of fertilizer; SR and precision nutrient management using Nutrient Expert tool; NE and GreenSeeker; (GS), applied using two methods; broadcasting (B) and drilling (D)) were investigated for multiple parameters. The results showed that NE, NE+GS, and SR-based nutrient management tactics with drilling improved crop yields, nutrient-use efficiency (NUE), and economic profitability relative to NE-broadcasting, SR broadcasting, and FFP broadcasting methods. Maize-wheat system productivity and net returns under NE+GS-drilling on PB were significantly higher by 31.2%, 49.7% compared to FFP-broadcasting method, respectively. Total global warming potential (GWP) was lower in the PB-based maize-wheat system coupled with precision nutrient management compared to CT-based maize-wheat system with FFP. Higher (15.2%) carbon sustainability index (CSI) was recorded with NE-drilling compared to FFP-broadcasting method. Results suggests that PB-based maize-wheat system together with precision nutrient management approaches (NE+GS+drilling) can significantly increase crop yields, NUE, and profitability while reducing the emission of greenhouse gases (GHGs) from maize-wheat systems in eastern Indo Gangetic Plains (IGP).
Performance of Different-Use Type Industrial Hemp Cultivars under Mid-Atlantic Region Conditions
Authors: Rahemi Alireza , Dhakal Ramesh , Temu Vitalis Wilbald , Rutto Laban , Kering Maru Kipleting
Published: November 2021

Due to recent classification as a commodity crop in North America, producer interest in industrial hemp (Cannabis sativa L.) has increased. In the Commonwealth of Virginia, there is a need to evaluate foreign-developed industrial hemp for its adaptability and to develop new cultivars suited to local climatic conditions. Eight cultivars (‘Bialobrzeskie’, ‘Canda’, ‘Fedora 17’, ‘Felina 32’, ‘Joey’, ‘Tygra’, ‘USO 31’, and ‘Wojko’) were evaluated for grain and five (‘Bialobrzeskie’, ‘Carmagnola’, ‘Fedora 17’, ‘Futura 75’, and ‘Wojko’) for vegetative biomass. An experiment carried out at the Virginia State Research and Demonstration (Randolph) farm was laid out in a randomized complete block design with three replications. The results show that cultivars differed in the cumulative growing degree days (CGDD) needed for growth phase transitions, with ‘USO 31’ having an early transition to the reproductive phase. In addition, ‘Fedora 17’ produced greater grain yield and may have the potential for greater performance with better management to improve its adaptability to local conditions. Across cultivars and years, the grain protein content averaged 241 g kg−1 and the mineral elements were at sufficient levels for animal and human nutrition. ‘Carmagnola’ produced greater biomass yield and has the potential for adoption as local fiber cultivar.
Regulating Seeds—A Challenging Task
Authors: Louwaars Niels , De Jonge Bram
Published: November 2021

Seed is an essential start of any crop production. Seed, as both botanical seed and vegetive planting materials, is thus a very important component of agricultural livelihoods in food, ornamental, and industrial value chains, of local and global food security, and a determinant of sustainability. All farmers need good seed, irrespective of the farming system and markets that they supply. Seed qualities, in terms of germination/vigour, health, and genetic content, are a concern of all farmers. Farmers have various ways to access seeds. With time, the diversity of farmers’ and formal seed systems have become increasingly refined and complex. Given the importance of seed, not just for farmers but for society at large, seeds have become subject to an increasing number of regulations that pursue different policy objectives. Some have been intentionally developed to regulate seed systems themselves, while others impact them as a side effect. Various components of different policies, regulations and outcomes, their interactions and apparent dilemmas and inconsistencies are discussed to highlight the significance of seeds and to illustrate the importance for policymakers and regulators to carefully phrase rules and be sensitive toward the possible unintended effects of their actions. This particularly relates to seed marketing regulations, intellectual property and farmers’ rights, and biodiversity and biosafety rules. A general conclusion is that rules and regulations need to respond to evolving technical and socio-economic developments. Since seed systems differ widely and operate side by side, regulating a particular system may negatively impact others. The challenge for policymakers is to create policies and regulations that support both formal and farmers’ seed systems where they are most effective while minimalising negative consequences for breeding, selection, and seed production in either system. Several suggestions and recommendations for how to do so are provided in this special issue.
Field-Evolved Sulfoxaflor Resistance of Three Wheat Aphid Species in China
Authors: Li Xinan , Wang Chao , Li Qiuchi , Zhu Saige , Tian Xujun , Zhang Yunhui , Li Xiangrui , Gao Haifeng , Liu Enliang , Wang Liande , Zhu Xun
Published: November 2021

Sulfoxaflor belongs to a new class of insecticides which are effective against many sap-feeding pests. Sitobion miscanthi, Rhopalosiphum padi, and Metopolophium dirhodum are the predominant pests coexisting on wheat plants. It is unknown whether these aphid species have developed resistance to sulfoxaflor. Here, the susceptibilities of three wheat aphid species from different regions of China to sulfoxaflor were evaluated. The results showed that two S. miscanthi, one R. padi, and two M. dirhodum field populations were highly resistant to sulfoxaflor. Additionally, 13 S. miscanthi, 9 R. padi, and 4 M. dirhodum field populations were moderately resistant to sulfoxaflor. Analysis of differences in toxicity showed that the susceptibility levels of R. padi in 9 of 20 regions, M. dirhodum in 5 of 9 regions, and M. dirhodum in 3 of 9 regions to sulfoxaflor were greater than those of S. miscanthi, S. miscanthi, and R. padi in the same regions, respectively. Thus, each wheat aphid species has field populations that are highly sulfoxaflor resistant. The R. padi and M. dirhodum populations were more susceptible to sulfoxaflor than those of S. miscanthi. These findings provide new insights into insecticide resistance development and rational sulfoxaflor use.
Effects of Nitrogen, Azoxystrobin and a Biostimulant Based on Brown Algae and Yeast on Wild Rocket Features at Harvest and During Storage
Authors: Schiattone Maria Immacolata , Boari Francesca , Cantore Vito , Castronuovo Donato , Denora Michele , Di Venere Donato , Perniola Michele , Renna Massimiliano , Sergio Lucrezia , Candido Vincenzo
Published: November 2021

Recently, the use of biostimulant substances of different origins has been affirmed. They act differently on the physiological processes of the plant, helping to improve its productive response and resistance to biotic and abiotic stress. Therefore, the response of the wild rocket to two substances known to have biostimulating activity (Azoxystrobin, and a fluid extract of brown algae and yeast), was evaluated. Two experimental trials (Exp 1 and Exp 2) were carried out in the greenhouse. The collected product, in addition to being evaluated from a qualitative point of view, was used for evaluation of shelf life. Exp 1 involved the comparison of two N levels with two Azoxystrobin levels (treated–Azo+, and untreated control). Exp 2 involved the comparison of two N levels, and two biostimulating substances based on Azoxystrobin (Azo+) and on fluid extracts of yeast and brown algae (YBA+), in addition to untreated control. A split-plot experimental design with three replications was used. Azo+ increased marketable yield of wild rocket by 16.8% and enhanced some qualitative features at harvest as the increase in chlorophyll (+17.8%) and carotenoids (+13.5%), and decrease in nitrates (−10.6%), regardless of the nitrogen level. Furthermore, Azo+ increased the shelf life (+2.5 days) of wild rocket stored at 3.5 °C. In particular, Azo+ slowed the loss of chlorophyll (yellowing) and the worsening of odor and visual appearance. As Azoxystrobin is a fungicide effective for the control of some diseases of wild rocket, its use should be promoted as it would offer not only the benefit of disease control but also improved production and shelf life. YBA+ caused an increase in the chlorophyll content (+12.5%) at harvest of wild rocket, but reduced its antioxidant activity (−40%). YBA+ did not cause substantial variations in shelf life with the exception of a slowdown in the degradation of carotenoids. Further research is desirable to evaluate other variables such as the dose and time of application.
Immunomodulatory Effects of Herbal Compounds Quercetin and Curcumin on Cellular and Molecular Functions of Bovine-Milk-Isolated Neutrophils toward Streptococcus agalactiae Infection
Authors: Disbanchong Purichaya , Punmanee Wichayaporn , Srithanasuwan Anyaphat , Pangprasit Noppason , Wongsawan Kanruethai , Suriyasathaporn Witaya , Chuammitri Phongsakorn
Published: November 2021

Herbal phytochemicals featuring active ingredients including quercetin and curcumin have shown potential in treating human and animal diseases. The current study investigated their potential function in vitro for host immunomodulation associated with Streptococcus agalactiae subclinical bovine mastitis via milk-isolated neutrophils. Our results showed a positive influence on cellular migration, reactive oxygen species (ROS) generation, phagocytosis, and bacterial killing as well as neutrophil extracellular traps (NETs) release. This study also highlighted several important molecular aspects of quercetin and curcumin in milk-isolated neutrophils. Gene expression analyses by RT-PCR revealed significant changes in the expression of proinflammatory cytokines (IL1B, IL6, and TNF), ROS (CYBA), phagocytosis (LAMP1), and migration (RAC). The expression levels of apoptotic genes or proteins in either pro-apoptosis (CASP3 and FAS) or anti-apoptosis (BCL2, BCL2L1, and CFLAR) were significantly manipulated by the effects of either quercetin or curcumin. A principal component analysis (PCA) identified the superior benefit of quercetin supplementation for increasing both cellular and molecular functions in combating bacterial mastitis. Altogether, this study showed the existing and potential benefits of these test compounds; however, they should be explored further via in vivo studies.
Bad Smells and Broken DNA: A Tale of Sulfur-Nucleic Acid Cooperation
Authors: Shackelford Rodney E. , Li Yan , Ghali Ghali E. , Kevil Christopher G.
Published: November 2021

Hydrogen sulfide (H2S) is a gasotransmitter that exerts numerous physiologic and pathophysiologic effects. Recently, a role for H2S in DNA repair has been identified, where H2S modulates cell cycle checkpoint responses, the DNA damage response (DDR), and mitochondrial and nuclear genomic stability. In addition, several DNA repair proteins modulate cellular H2S concentrations and cellular sulfur metabolism and, in turn, are regulated by cellular H2S concentrations. Many DDR proteins are now pharmacologically inhibited in targeted cancer therapies. As H2S and the enzymes that synthesize it are increased in many human malignancies, it is likely that H2S synthesis inhibition by these therapies is an underappreciated aspect of these cancer treatments. Moreover, both H2S and DDR protein activities in cancer and cardiovascular diseases are becoming increasingly apparent, implicating a DDR–H2S signaling axis in these pathophysiologic processes. Taken together, H2S and DNA repair likely play a central and presently poorly understood role in both normal cellular function and a wide array of human pathophysiologic processes. Here, we review the role of H2S in DNA repair.
Non-Enzymatic Amperometric Glucose Screen-Printed Sensors Based on Copper and Copper Oxide Particles
Authors: Guati Carlota , Gomez-Coma Lucía , Fallanza Marcos , Ortiz Inmaculada
Published: November 2021

Non-enzymatic amperometric glucose sensors have gained much attention in the past decade because of the better chemical and thermal stability and biocompatibility compared to conventional sensors based on the use of biomolecules. This study focuses on a novel copper and copper oxide-based glucose sensor synthesized by an electrodeposition technique through a rigorous protocol which reports an excellent analytical performance due to its structure and its increased active area. In addition, the linear response range, detection limit and sensitivity were 0.5–5.0 mmol L−1, 0.002 mmol L−1, 904 μA mmol−1 L−1 cm−2, respectively. Results show a reliable electrode as it is chemically stable, exhibits rapid and excellent sensitivity, and it is not significantly affected by coexisting species present in the blood samples; furthermore, it reports a maximum relative standard deviation error (RSD) of 6%, and showed long operating life as the electrode was used for thousand measurements of 4.0 mmol L−1 glucose solution during three days.
Highly Efficient Transformerless Inverter with Flying-Capacitor Buck–Boost for Single-Phase Grid-Connected PV Systems
Authors: Salem Ali , Sedraoui Khaled
Published: November 2021

Grid-connected inverters (GCI) are commonly used in PV system applications to generate a regulated AC current to feed into the grid. Transformerless inverters are the most advanced inverters that are used in industry, which provide efficiency with smaller size and lower cost. This paper proposes a grid-connected single-phase transformerless inverter with the technology of common ground and the virtual DC bus concept. In this topology, the grid neutral is connected directly to the PV ground, which generates a constant common mode voltage (CMV), thus leading to the elimination of the leakage current caused by the PV array’s parasitic capacitance. The proposed inverter has a buck–boost circuit with a flying capacitor to generate the DC bus for a negative power cycle, four switches, and two diodes. A unipolar sinusoidal pulse width modulation (SPWM) technique is used which reduces the output filter requirements. In addition, only one switch carries the load current during the active states of both the negative and positive power cycle, thus minimizing the conduction losses. One more advantage presented in the proposed inverter is its ability to charge the flying capacitor during all operation states due to the existence of the buck–boost circuit. Design and theoretical calculations were conducted in this paper to optimize the losses. Moreover, the PSIM simulation was used to validate the proposed topology inverter, verify the performance by showing leakage current elimination, and achieve unipolar voltage in the output bus. The simulation results show a peak efficiency of 98.57% for a 2 kW inverter, which agrees with the theoretical calculations.
Local Failure Modes and Critical Buckling Loads of a Meta-Functional Auxetic Sandwich Core for Composite Bridge Bearing Applications
Authors: Sengsri Pasakorn , Kaewunruen Sakdirat
Published: November 2021

This paper presents a novel meta-functional auxetic unit (MFAU) cell designed to improve performance and weight ratio for structural bridge bearing applications. Numerical investigations were conducted using three-dimensional finite element models validated by experimental results. The validated models were exposed to compression and buckling actions to identify structural failure modes, with special attention placed on the global behaviours of the meta-functional auxetic (MFA) composite bridge bearing. This bearing uses an unprecedented auxetic sandwich core design consisting of multiple MFAU cells. Numerical predictions of the elastic local critical buckling loads of the MFAU cell were in excellent agreement with both the analytical and experimental results, with an observed discrepancy of less than 1%. These results demonstrate that local buckling failures of MFAU cells can potentially be incurred prior to yielding under compression due to their slenderness ratios. Surprisingly, the designed sandwich core used in the MFA composite bridge bearing model can mimic an auxetic structure with significant crashworthiness, implying that this novel core composite structure can be tailored for structural bridge bearing applications. Parametric studies were thus carried out in order to enrich our insight into the MFA composite elements. These insights, stemming from both experimental and numerical studies, enable a novel design paradigm for MFAU that can significantly enhance the structural performance of MFA composite bridge bearings in practice.
Numerical Investigation of Heat Transfer on Unsteady Hiemenz Cu-Water and Ag-Water Nanofluid Flow over a Porous Wedge Due to Solar Radiation
Authors: Inayat Usman , Iqbal Shaukat , Manzoor Tareq , Zia Muhammad Fahad
Published: November 2021

Nanoparticles are generally used to scatter and absorb solar radiations in nanofluid-based direct solar receivers to efficiently transport and store the heat. However, solar energy absorption in nanofluid can be enhanced by using differential materials and tuning nanofluid parameter. In this regard, theoretical investigations of unsteady homogeneous Hiemenz flow of an incompressible nanofluid having copper and silver nanoparticles over a porous wedge is carried out by using optimal homotopy asymptotic method (OHAM). Hence, a semi-analytical solver is applied to the transformed system to study the significance of magnetic field along with Prandtl number. In this work, impacts of conductive radiations, heat sink/source, unsteadiness, and flow parameters have been investigated for velocity and temperature profiles of copper and silver nanoparticles-based nanofluid. The effects of magnetic strength, volume fraction of nanoparticles, thermal conductivity, and flow parameters have also been studied on the considered nanofluids.
Response of Mechanical Properties of Polyvinyl Chloride Geomembrane to Ambient Temperature in Axial Tension
Authors: Zhang Xianlei , Ma Zhongyang , Wu Yunyun , Liu Jianqun
Published: November 2021

Aiming at the mechanical response of geomembrane (GEM) in membrane-faced rockfill dam (MFRD) to different ambient temperatures, the mechanical properties in axial tension of polyvinyl chloride (PVC) GEM were studied by experiment and theoretical analysis. First, fifteen groups of axial tensile tests for longitudinal/transverse specimens were conducted at different temperatures in the temperature environment laboratory, the stress–strain curve and Young’s modulus were obtained, and the variation of Young’s modulus with temperature was analyzed by Boltzmann function fitting. Second, the glass transition temperature of PVC GEM was obtained by differential scanning calorimetry (DSC), and the difference in mechanical properties between longitudinal and transverse specimens of PVC GEM was analyzed by thermomechanical analyzer (TMA) thermodynamic test. The results showed that the lower the temperature, the greater the Young’s modulus, and the smaller the linear interval of stress and strain, while the higher the temperature, the result is opposite. The difference in mechanical properties between the two directions is related to the ambient temperature. The orientation of polymer structure accounts for the difference in mechanical properties by theoretical analysis. The fitting results of Boltzmann function have a certain reference value for numerical simulation. In design of the membrane impervious structure in MFRD, the ambient temperature should be considered fully, and the longitudinal/transverse welding splicing should be avoided as far as possible. The current test specification should test the mechanical performance of GEM at normal operating temperature of reservoir instead of the test and quality evaluation at a single temperature. The temperature should be considered comprehensively in construction to avoid damaging the performance of impervious structure and ensure the service life.
Towards Explainable Augmented Intelligence (AI) for Crack Characterization
Authors: Fradkin Larissa , Uskuplu Altinbasak Sevda , Darmon Michel
Published: November 2021

Crack characterization is one of the central tasks of NDT&E (the Non-destructive Testing and Evaluation) of industrial components and structures. These days data necessary for carrying out this task are often collected using ultrasonic phased arrays. Many ultrasonic phased array inspections are automated but interpretation of the data they produce is not. This paper offers an approach to designing an explainable AI (Augmented Intelligence) to meet this challenge. It describes a C code called AutoNDE, which comprises a signal-processing module based on a modified total focusing method that creates a sequence of two-dimensional images of an evaluated specimen; an image-processing module, which filters and enhances these images; and an explainable AI module—a decision tree, which selects images of possible cracks, groups those of them that appear to represent the same crack and produces for each group a possible inspection report for perusal by a human inspector. AutoNDE has been trained on 16 datasets collected in a laboratory by imaging steel specimens with large smooth planar notches, both embedded and surface-breaking. It has been tested on two other similar datasets. The paper presents results of this training and testing and describes in detail an approach to dealing with the main source of error in ultrasonic data—undulations in the specimens’ surfaces.
The Maximal Complexity of Quasiperiodic Infinite Words
Authors: Staiger Ludwig
Published: November 2021

A quasiperiod of a finite or infinite string is a word whose occurrences cover every part of the string. An infinite string is referred to as quasiperiodic if it has a quasiperiod. We present a characterisation of the set of infinite strings having a certain word q as quasiperiod via a finite language Pq consisting of prefixes of the quasiperiod q. It turns out its star root Pq* is a suffix code having a bounded delay of decipherability. This allows us to calculate the maximal subword (or factor) complexity of quasiperiodic infinite strings having quasiperiod q and further to derive that maximally complex quasiperiodic infinite strings have quasiperiods aba or aabaa. It is shown that, for every length l≥3, a word of the form anban (or anbban if l is even) generates the most complex infinite string having this word as quasiperiod. We give the exact ordering of the lengths l with respect to the achievable complexity among all words of length l.
Two-Dimensional Gel Electrophoresis to Study the Activity of Type IIA Topoisomerases on Plasmid Replication Intermediates
Authors: Cebrián Jorge , Martínez Victor , Hernández Pablo , Krimer Dora B. , Fernández-Nestosa María-José , Schvartzman Jorge B.
Published: November 2021

DNA topoisomerases are the enzymes that regulate DNA topology in all living cells. Since the discovery and purification of ω (omega), when the first were topoisomerase identified, the function of many topoisomerases has been examined. However, their ability to relax supercoiling and unlink the pre-catenanes of partially replicated molecules has received little attention. Here, we used two-dimensional agarose gel electrophoresis to test the function of three type II DNA topoisomerases in vitro: the prokaryotic DNA gyrase, topoisomerase IV and the human topoisomerase 2α. We examined the proficiency of these topoisomerases on a partially replicated bacterial plasmid: pBR-TerE@AatII, with an unidirectional replicating fork, stalled when approximately half of the plasmid had been replicated in vivo. DNA was isolated from two strains of Escherichia coli: DH5αF’ and parE10. These experiments allowed us to assess, for the first time, the efficiency of the topoisomerases examined to resolve supercoiling and pre-catenanes in partially replicated molecules and fully replicated catenanes formed in vivo. The results obtained revealed the preferential functions and also some redundancy in the abilities of these DNA topoisomerases in vitro.
Co-Evolution of Breast Milk Lipid Signaling and Thermogenic Adipose Tissue
Authors: Röszer Tamás
Published: November 2021

Breastfeeding is a unique and defining behavior of mammals and has a fundamental role in nourishing offspring by supplying a lipid-rich product that is utilized to generate heat and metabolic fuel. Heat generation from lipids is a feature of newborn mammals and is mediated by the uncoupling of mitochondrial respiration in specific fat depots. Breastfeeding and thermogenic adipose tissue have a shared evolutionary history: both have evolved in the course of homeothermy evolution; breastfeeding mammals are termed “thermolipials”, meaning “animals with warm fat”. Beyond its heat-producing capacity, thermogenic adipose tissue is also necessary for proper lipid metabolism and determines adiposity in offspring. Recent advances have demonstrated that lipid metabolism in infants is orchestrated by breast milk lipid signals, which establish mother-to-child signaling and control metabolic development in the infant. Breastfeeding rates are declining worldwide, and are paralleled by an alarming increase in childhood obesity, which at least in part may have its roots in the impaired metabolic control by breast milk lipid signals.
Epigenetic Therapy Augments Classic Chemotherapy in Suppressing the Growth of 3D High-Grade Serous Ovarian Cancer Spheroids over an Extended Period of Time
Authors: Bilbao Michelle , Katz Chelsea , Kass Stephanie L. , Smith Devon , Hunter Krystal , Warshal David , Aikins James K. , Ostrovsky Olga
Published: November 2021

Recurrent high-grade serous ovarian cancer (HGSC) is clinically very challenging and prematurely shortens patients’ lives. Recurrent ovarian cancer is characterized by high tumor heterogeneity; therefore, it is susceptible to epigenetic therapy in classic 2D tissue culture and rodent models. Unfortunately, this success has not translated well into clinical trials. Utilizing a 3D spheroid model over a period of weeks, we were able to compare the efficacy of classic chemotherapy and epigenetic therapy on recurrent ovarian cancer cells. Unexpectedly, in our model, a single dose of paclitaxel alone caused the exponential growth of recurrent high-grade serous epithelial ovarian cancer over a period of weeks. In contrast, this effect is not only opposite under treatment with panobinostat, but panobinostat reverses the repopulation of cancer cells following paclitaxel treatment. In our model, we also demonstrate differences in the drug-treatment sensitivity of classic chemotherapy and epigenetic therapy. Moreover, 3D-derived ovarian cancer cells demonstrate induced proliferation, migration, invasion, cancer colony formation and chemoresistance properties after just a single exposure to classic chemotherapy. To the best of our knowledge, this is the first evidence demonstrating a critical contrast between short and prolonged post-treatment outcomes following classic chemotherapy and epigenetic therapy in recurrent high-grade serous ovarian cancer in 3D culture.
Comparison of Characteristics and Survival Rates of Resectable Pancreatic Ductal Adenocarcinoma according to Tumor Location
Authors: Sung Min Kyu , Park Yejong , Kwak Bong Jun , Jun Eunsung , Lee Woohyung , Song Ki Byung , Lee Jae Hoon , Hwang Dae Wook , Kim Song Cheol
Published: November 2021

The impact of tumor location on patient survival in pancreatic ductal adenocarcinoma (PDAC) remains controversial. This study investigated the association between primary tumor location and survival rates for resectable PDAC. Additionally, we assessed if this association remains consistent across categories of the Tumor-Node-Metastasis staging system. We analyzed 2471 patients who underwent surgical resection between 2000 and 2018 at a single center. Subgroup analysis was performed according to the Tumor-Node-Metastasis staging system. Among the group, 67.9% (1677 patients) had pancreatic head cancer (PHC) and 32.1% (794 patients) had pancreatic body/tail cancer (PBTC). Patients with PHC had worse overall survival and worse disease-free survival than those with PBTC. Patients with PHC had worse survival in stage IB and stage IIB than those with PBTC. No significant difference was observed for stages IA, IIA, and III. Multivariate analysis showed that elevated CA 19-9, mGPS, a longer hospital stay, complication, accompanying vein resection, larger tumor size, worse differentiation, higher TNM stage (stage IIB, III, IV), presence of LVI, and positive resection margin were risk factors for poor survival after resection. In resectable PDAC, patients with PHC had worse overall and disease-free survival than those with PBTC. However, tumor location was not an independent prognostic factor for PDAC.
Serum Vitamin D as a Marker of Impaired Information Processing Speed and Early Disability in Multiple Sclerosis Patients
Authors: Virgilio Eleonora , Vecchio Domizia , Crespi Ilaria , Barbero Paolo , Caloni Beatrice , Naldi Paola , Cantello Roberto , Dianzani Umberto , Comi Cristoforo
Published: November 2021

Slowed information processing speed (IPS) is the hallmark and first cognitive domain to be altered in multiple sclerosis (MS) patients. Insufficient serum vitamin D was previously associated with disease development, relapses, and progression, but little is reported on cognition. However, vitamin D and cognitive impairment (CI) in other neurodegenerative diseases have already been linked. We explored the possible correlation between vitamin D and IPS at diagnosis and early disability at last follow-up in 81 MS patients. At diagnosis, we collected vitamin D levels and performed a Symbol Digit Modalities Test (SDMT). Raw scores were adjusted for age, gender, and educational level. Early disability was evaluated with MS severity score (MSSS) and age-related MSSS (ARMSS). A total of 71 patients (86.58%) showed hypovitaminosis D (19.71 ± 8.76 ng/mL) and 18 patients (21.95%) had CI. Patients with CI showed severe hypovitaminosis D (p = 0.004). No patients with sufficient vitamin D levels had CI. We found a positive correlation between vitamin D levels at diagnosis and (1) SDMT raw and z-score that persisted after correction for sunlight exposure and MRI baseline characteristics, and (2) EDSS, MSSS, and ARMSS after a mean 2 year follow-up. Low vitamin D levels may affect both cognition and early disability in newly diagnosed MS patients.
Catalytic Prebiotic Formation of Glycerol Phosphate Esters and an Estimation of Their Steady State Abundance under Plausible Early Earth Conditions
Authors: Gull Maheen , Pasek Matthew A.
Published: November 2021

The emergence of biological phosphate esters of glycerol could have been a crucial step in the origin and evolution of life on the early Earth as glycerol phosphates today play a central role in biochemistry. We investigate here the formation of the glycerol phosphates by employing various rock samples, salts, and minerals as potential catalysts to aid the phosphorylation process. We report the synthesis of various phosphate esters of glycerol including glycerol-1-phosphate, glycerol-2-phosphate, cyclic glycerol-monophosphate as well as various diphosphate esters. Furthermore, the decomposition rates of glycerol phosphate under mild heating were also studied while keeping the pH constant. It was observed that glycerol phosphate starts decomposing quickly under mild heating conditions into inorganic orthophosphate and pyrophosphate, and a steady state concentration of ~0.5 M of glycerol phosphate may have been reasonable in ponds with abundant glycerol, phosphate, urea, and catalytic minerals.
Photoelectrochemical Conversion of Methane into Value-Added Products
Authors: Mehmood Adeel , Chae Sang Youn , Park Eun Duck
Published: November 2021

Methane has been reported to be directly converted into value-added products through various methods. Among them, photoelectrochemical (PEC) methane conversion is considered an eco-friendly method because it utilizes solar light and is able to control the selectivity to different products by means of application of an external bias. Recently, some PEC methane conversion systems have been reported, but their performance efficiencies are relatively lower than those of other existing thermal, photocatalytic, and electrochemical systems. The detailed mechanism of methane activation is not clear at this stage. In this review, various catalytic materials and their roles in the reaction pathways are summarized and discussed. Furthermore, promising semiconductor materials, co-catalysts, and oxidants have also been proposed. Finally, direct and indirect pathways in the design of the PEC methane conversion system have been discussed.
Interaction between Non-Coding RNAs and Androgen Receptor with an Especial Focus on Prostate Cancer
Authors: Taheri Mohammad , Khoshbakht Tayyebeh , Jamali Elena , Kallenbach Julia , Ghafouri-Fard Soudeh , Baniahmad Aria
Published: November 2021

The androgen receptor (AR) is a member of the nuclear receptor superfamily and has three functional domains, namely the N-terminal, DNA binding, and C-terminal domain. The N-terminal domain harbors potent transactivation functions, whereas the C-terminal domain binds to androgens and antiandrogens used to treat prostate cancer. AR has genomic activity being DNA binding-dependent or through interaction with other DNA-bound transcription factors, as well as a number of non-genomic, non-canonical functions, such as the activation of the ERK, AKT, and MAPK pathways. A bulk of evidence indicates that non-coding RNAs have functional interactions with AR. This type of interaction is implicated in the pathogenesis of human malignancies, particularly prostate cancer. In the current review, we summarize the available data on the role of microRNAs, long non-coding RNAs, and circular RNAs on the expression of AR and modulation of AR signaling, as well as the effects of AR on their expression. Recognition of the complicated interaction between non-coding RNAs and AR has practical importance in the design of novel treatment options, as well as modulation of response to conventional therapeutics.
Quantitative Evaluation of Cardiac Cell Interactions and Responses to Cyclic Strain
Authors: Tran Richard Duc Hien , Morris Tessa Altair , Gonzalez Daniela , Hetta Ali Hatem Salaheldin Hassan Ahmed , Grosberg Anna
Published: November 2021

The heart has a dynamic mechanical environment contributed by its unique cellular composition and the resultant complex tissue structure. In pathological heart tissue, both the mechanics and cell composition can change and influence each other. As a result, the interplay between the cell phenotype and mechanical stimulation needs to be considered to understand the biophysical cell interactions and organization in healthy and diseased myocardium. In this work, we hypothesized that the overall tissue organization is controlled by varying densities of cardiomyocytes and fibroblasts in the heart. In order to test this hypothesis, we utilized a combination of mechanical strain, co-cultures of different cell types, and inhibitory drugs that block intercellular junction formation. To accomplish this, an image analysis pipeline was developed to automatically measure cell type-specific organization relative to the stretch direction. The results indicated that cardiac cell type-specific densities influence the overall organization of heart tissue such that it is possible to model healthy and fibrotic heart tissue in vitro. This study provides insight into how to mimic the dynamic mechanical environment of the heart in engineered tissue as well as providing valuable information about the process of cardiac remodeling and repair in diseased hearts.