bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan Pola Komunikasi Soft Selling melalui Storytelling dalam Film Iklan “Metamorfodream”
Authors: Dewi Nuria Indah Kurnia , Saraswati Agni , Furqon Audrey Nafisa Falihah
Published: November 2022

Abstrak Prodi D3 Animasi ISI Yogyakarta (Prodi Animasi) sebagai lembaga pendidikan pencetak pekerja seni dalam bidang animasi perlu melakukan proses diseminasi yang berkesinambungan sebagai upaya untuk meningkatkan animo masyarakat. Terkait tujuan tersebut, Prodi Animasi memerlukan sebuah media diseminasi komunikasi yang relevan dengan perkembangan zaman, sesuai dengan latar belakang target audiens, serta mampu merepresentasikan karakter Prodi Animasi itu sendiri. Berdasarkan kriteria tersebut, maka film iklan animasi berjudul “Metamorfodream” dipilih sebagai sebuah model media diseminasi. Film iklan ini dirancang dengan memadukan unsur storytelling dan periklanan sehingga menghasilkan iklan dengan pola komunikasi soft selling. Landasan teori yang digunakan adalah teori film animasi oleh Jean Ann Wright dan konsep mengenai identitas brand oleh Derrick Chong serta teori penceritaan Struktur Tiga Babak oleh Weels Root. Metode penciptaan yang dipakai adalah proses penciptaan kreatif oleh Graham Wallas. Tujuan penelitian ini difokuskan untuk mengetahui bagaimana penerapan pola komunikasi soft selling melalui storytelling dalam film iklan Metamorfodream, di mana unsur storytelling akan digunakan dalam membangun bahasa komunikasi sehingga iklan yang dihasilkan akan bersifat soft selling sehingga dapat dinikmati sebagai sebuah karya film. Kata Kunci: diseminasi, film iklan animasi, storytelling, soft selling AbstractAs an educational institution that produces art workers, the Animation Program in the Indonesia Institute of the Arts Yogyakarta needs to carry out a continuous dissemination process to increase public interest. Related to this goal, the Animation Program requires a communicative dissemination medium that is relevant to the times and suitable to the audience's background. It should be able to represent the character of the Animation Study Program itself. Based on these criteria, the animated advertising film entitled “Metamorphodream” was chosen as a media dissemination model. This advertising film is designed by combining storytelling and advertising elements, so as to produce advertisements with soft-selling communication type. The theoretical basis used is the theory of animated films by Jean Ann Wright, the concept of brand identity by Derrick Chong, and the theory of storytelling in Three Acts Structure by Weels Root. The method of creation used is the creative creation process by Graham Wallas. This research aims to know how soft- selling communication patterns can be applied through storytelling in the Metamorphodream advertising film. Storytelling elements will be used in building a communication language so that the resulting advertisements will be soft selling so that they can be enjoyed as a film. Keywords: animated advertising films, concept art, dissemination, storytelling
Analisis Sentimen Terhadap Kementrian Perdagangan Pada Sosial Media Twitter Menggunakan Metode Naïve Bayes
Authors: Hasibuan Fefbiansyah , Priatna Wowon , Lestari Tyastuti Sri
Published: November 2022

Penelitian ini untuk mendapatkan opini masyarakat di media sosial twitter terkait tentang Kementrian Perdagangan Republik Indonesia dengan mengimplementasikan algoritma Naïve Bayes untuk melakukan analisis sentimen terhadap opini yang ada. Penelitian ini bertujuan untuk menganalisis opini masyarakat di media sosial terhadap Kementrian Perdagangan Republik Indonesia mengenai kelangkaan minyak goreng dengan menggunakan Naïve Bayes. Data yang digunakan pada penelitian ini adalah postingan tweet yang diberikan masyarakat yang ditujukan dan berkaitan kepada Kementrian Perdagangan Republik Indonesia yang diambil sebanyak 1000 tweet. Metode pengumpulan data dilakukan dengan cara crawling menggunakan acces token api key yang di dapat dari twitter develover. Setelah data didapat maka dilakukan text processing agar mempermudah dalam proses analisis. Hasil analisis pada penelitian ini menggunakan  algoritma naïve bayes adalah  dengan nilai akurasi sebesar 89,24%. Perbandingan persentase didapatkan 84,02% tanggapan yangdiberikan masyarakat bernilai positif dan 15,98% bernilai negatif.
Penilaian Esai Pendek Otomatis Berdasarkan Similaritas Semantik dengan SBERT
Authors: Chamidah Nurul , Santoni Mayanda Mega , Irmanda Helena Nurramdhani , Astriratma Ria , Yulnelly Yulnelly
Published: November 2022

Ujian dalam bentuk soal esai dianggap lebih baik dalam mengukur pemahaman dari pada soal berbentuk pilihan. Namun, jawaban esai memerlukan waktu dan tenaga lebih banyak untuk dievaluasi dan sering terjadi inkonsistensi. Maka dari itu, diperlukan suatu sistem penilaian esai otomatis yang dapat membantu evaluator dalam memberikan nilai dengan lebih cepat dan lebih konsisten. Penelitian ini bertujuan untuk mengevaluasi performa model penilaian esai otomatis dimana teks esai jawaban uji dan kunci jawaban dibandingkan secara semantik untuk mengetahui seberapa besar persamaan antara teks jawaban uji dan kunci jawaban. Semantik dari teks esai diperoleh dengan melakukan word embeddings dengan memanfaatkan model bahasa pretrained Siamese-BERT (SBERT) yang mentransformasi teks esai menjadi vektor sepanjang 512. Proses penilaian esai otomatis ini dimulai dengan praproses pada teks dengan menerapkan case folding, berikutnya word embeddings pada teks yang telah di praproses dengan SBERT. Vektor numerik dari kunci jawaban dan jawaban uji hasil word embeddings kemudian dibandingkan dengan Cosine Similarity untuk mendapatkan similaritas semantik sekaligus nilai esai yang merupakan output model. Evaluasi model penilaian esai otomatis ini dilakukan dengan membandingkan nilai dari model dengan nilai dari evaluator manusia. Pengukuran yang dipakai untuk mengukur performa penilaian esai otomatis ini adalah adalah dengan menghitung Mean Absolute Error (MAE) dan Pearson Correlation, dimana hasil penelitian ini menunjukan nilai rata-rata MAE sebesar 0.26 dan rata-rata korelasi sebesar 0.78.
Information Retrieval Pada Frequently Asked Questions (FAQ) dengan metode String Similarity
Authors: Setiawan Gede Herdian , Adnyana I Made Budi
Published: November 2022

Information retrieval merupakan sebuah sarana untuk menemukan informasi berdasarkan kumpulan informasi pada data terstruktur maupun tidak terstruktur secara otomatis. implementasi information retrival seperti mesin pencari menggunakan query dari pengguna dengan bahasa alami manusia kemudian sistem dapat menemukan dokumen atau informasi yang berkaitan dengan query dari pengguna. Pada penelitian ini di usulkan sistem information retrieval pada Frequently Asked Questions atau FAQ dengan mencari pertanyaan yang mirip (similar) pada daftar pertanyaan di basis data terhadap pertanyaan yang diberikan oleh pengguna menggunakan algoritma Cosine similarity untuk mencari kesamaan kosinus tertinggi. Selanjutnya memberikan respon jawaban yang sebelum nya sudah di berikan label terhadap pertanyaan yang relevan dan memiliki similaritas paling tinggi. Telah dihasilkan dataset FAQ dan dilakukan preprocessing, penerapan algoritma Cosine Similarity terhadap input pertanyaan (query) dengan dataset dan menghasilkan bobot pada setiap pertanyaan (label) pada dataset. Melalui evaluasi akurasi pemberian bobot similaritas yang dilakukan dengan memberikan sembilan input pertanyaan dibagai pada tiga kategori berdasarkan tingkat kemiripan memiliki akurasi mencapai 100%, dengan demikian information retrieval dengan Cosine similarity telah mampu memberikan bobot sesuai dengan tingkat similaritas pertanyaan (query) dengan dataset pertanyaan pada FAQ
Implementasi Arm Robot pada Smart Farming Berbasis Internet of Things
Authors: Cahya Waskita , Febriansyah Muhammad , Angellia Filda , Widyaningsih Tri Wahyu
Published: November 2022

Hidroponik merupakan metode penanaman tanpa media tanah yang mampu meningkatkan kuantitas hasil tanam dengan lahan minimal. Smart farming menerapkan teknologi dalam pertanian seperti big data, internet of things dan cloud computing. Dalam penelitian ini menerapkan teknologi IoT dengan menggunakan arm robot dan robot slider. Beberapa sensor yang digunakan untuk mengukur kualitas tanaman hidroponik antara lain sensor TDS untuk mendeteksi nutrisi, pH, dan DHT 11 untuk mendeteksi suhu dan kelembapan. Penelitian ini diawali dengan pembibitan, penanaman, pemantauan nutrisi, dan penuaian tanaman hidroponik. Perancangan sistem smart farming ini menggunakan diagram deployment, diagram objek dan diagram flowchart. Sistem smart farming ini mampu mendeteksi dan memonitoring tanaman hidroponik dengan menerapkan teknologi IoT, menerapkan arm robot untuk melakukan penanaman, penyiraman ,dan proses tuai secara otomatis, serta dapat membantu petani urban dalam mengelola pertanian meskipun dalam lahan yang terbatas.
Implementasi Data Mining Pada Perpustakaan Untuk Penentuan Tata Letak Buku Dalam Menarik Minat Baca
Authors: Gama Adie Wahyudi Oktavia , Widnyani Ni Made , Suparna Putu
Published: November 2022

Perpustakaan memiliki sistem informasi untuk mempermudah manajemen sirkulasi buku. Sistem informasi biasanya hanya menghasilkan laporan harian, mingguan atau bahkan bulanan saja. Data mining adalah suatu cara dalam menemukan pengetahuan baru pada basis data dari suatu sistem informasi. Data mining mampu mengurai basis data untuk melakukan ekstraksi pengetahuan yang tidak ditemukan sebelumnya oleh sistem informasi. Asosiasi merupakan salah satu metode data mining yang bisa dipakai untuk menggali suatu pengetahuan baru. Teknik asosiasi memiliki berbagai algoritma yang dapat digunakan sebagai pendekatan dalam membentuk kombinasi produk, salah satunya adalah menggunakan algoritma apriori. Apriori berjalan dengan melakukan pendekatan dengan iterasi berulang pada basis data, dimana k-itemset dipakai membentuk (k+1)-itemset selanjutnya. Aturan asosiasi dimulai dengan penyiapan basis data serta setting nilai batas support dan confidence. Apriori melakukan scaning basis data secara berulang untuk membuat kombinasi dan mencatat kemunculannya pada semua transaksi. Frequent itemset atau kombinasi yang paling sering muncul diambil dari itemset yang total kemuculannya lebih besar atau sama dengan ambang nilai support yang ditentukan. Data yang dipakai pada riset ini adalah sampel 100 data peminjaman dari basis data sistem informasi perpustakaan Universitas Pendidikan Nasional. Terdapat 42 judul buku yang memenuhi minimum support, berhasil dibentuk 40 aturan asosiasi yang sesuai memenuhi minimum confidence. Berdasarkan analisis dan pembahasan tersebut diatas maka dapat dilihat bahwa algoritma apriori dapat menemukan frequent itemset tersembunyi dari data peminjaman buku yang digunakan untuk membentuk aturan asosiasi. Aturan asosiasi yang dihasilkan bisa dipakai mendukung keputusan penataan buku yang baik guna meningkatkan minat baca anggota perpustakaan.
Perbandingan Elemen Diegetik Dan Non Diegetik Terhadap Game Experience Pada Game Matematika
Authors: Sholikhah Amirotus , Rizky .
Published: November 2022

Kegiatan pembelajaran yang dilakukan secara daring atau School From Home (SFH) melibatkan banyak pihak untuk memudahkan para siswa dalam belajar. Banyak media atau aplikasi game yang dibuat untuk proses belajar mengajar para siswa seperti aplikasi game edukasi pada mata pelajaran matematika. Penelitian ini bertujuan untuk menganalisa dan membandingkan pengalaman bermain game pada elemen diegetik dan non diegetik terhadap game matematika. Metode penelitian dalam membandingkan game ini menggunakan metode CEGE yang terdiri dari beberapa bagian yaitu enjoyment, frustration, Puppetry, dan bagian Video-Game. Pengujian dilakukan dengan mengisi kuesioner yang berjumlah 38 pertanyaan terhadap 22 responden. Berdasarkan hasil pengujian, sebagian besar pengguna memilih game dengan elemen diegetik pada skala enjoyment dengan nilai 16 dan CEGE dengan nilai 146, sedangkan pada game non diegetik sebagian besar memilih pada skala video-game (environment) dengan nilai 29. Sehingga hasil penelitian ini menunjukkan bahwa user lebih banyak menggunakan elemen diegetik.
Microneedling opportunities in acne and postacne treatment
Authors: Novikov K. A.
Published: November 2022

Postacne is one of the leading problems in aesthetic medicine. Often, the presence of postacne elements is combined with ongoing acne, which together creates conditions for social maladaptation, a decrease in self-esteem, and the patient’s desire for social isolation. Currently, the issue of postacne correction remains open and not fully resolved. The difficulties of correction, in particular atrophic postacne scars, lie in the depletion of the skin after a long inflammatory process and a decrease in skin resources that ensure full regeneration. The thickness of the dermis of the skin of the face and the depth of the postacne scars create a pronounced optical defect, which often demonstrates resistance to correction by fractional laser radiation, chemical and laser peels. In this article, microneedling is considered as one of the options for correcting atrophic scars post-acne, by stimulating the synthesis of collagen in the atrophic scar, hanging the regenerative abilities of the skin, the formation of organotypic healing, and so accelerating the resorption of postinflammatory stagnant spots. Also, the use of microneedling contributes to the resolution of inflammatory elements and the prolongation of acne remission.
Experience of using acitretin in genital form of scleroatrophic lichen with urethral lesion in men
Authors: Ignatovsky A. V.
Published: November 2022

The defeat of the genitals in scleroatrophic lichen is often accompanied by the involvement of the urethra in the process, which threatens serious complications and, first of all, the development of stricture. Therapy of such cases is difficult and ineffective in the case of topical corticosteroids. The article describes the positive experience of using acitretin at a dose of 25 mg per day for the treatment of scleroatrophic lichen, which occurs with damage to the external orifice and anterior segment of the urethra.
TR 61 Bölgesindeki İş İlanlarında Kadın İstihdamı | Women Employment in Job Advertisements in TR61 Region
Authors: ÇETİNKAYA BOZKURT Özlem , KIRAN Funda
Published: November 2022

Bu çalışmada, kadın çalışan istihdamına yönelik çıkan iş ilanlarının işe alım yapılacağı departmanlar, pozisyon seviyeleri, sektörler ile kadınlardan talep edilen tecrübe durumu, eğitim seviyesi, çalışma şekli ve eril/dişil özellikler saptanarak, toplanan verilerin karşılaştırılması amaçlanmıştır. Bu doğrultuda nitel araştırma yöntemi kullanılmıştır. Evren, Kariyer.net portalından ulaşılan TR 61 (Batı Akdeniz) bölgesindeki kadın çalışan arayan iş ilanları olarak belirlenmiş ve 10 Aralık 2019 -10 Ocak 2020 tarihleri arasında adı geçen sitede yer alan iş ilanları incelenmiştir. Toplam 28 ilan çalışma kapsamına dahil edilmiştir. Nicel içerik analizinin gerçekleştirildiği çalışmada MAXQDA 18.0 programı kullanılmıştır. Sonuç olarak sadece kadın çalışanların talep edildiği ilanların incelendiği çalışmada, adayın sahip olması gerektiği kriterlerde eril özelliklere de ulaşılmıştır. Hatta üç adet ilanda hiç dişil özelliğe yer verilmediği aksine eril özelliklere yer verildiği tespit edilmiştir. Dişil özellikler arasında iletişim becerilerine sahip olmanın en çok aranan özellik olduğu dikkat çekmektedir. Bu özellik tüm pozisyon seviyelerinde ve eğitim düzeylerinde yer almaktadır. Kadınların özellikle turizm, sağlık, spor ve gıda gibi emek-yoğun sektörlerde istihdam edildiği tespit edilmiştir. İşe alımın yapılacağı pozisyonun daha çok pazarlama, muhasebe, kasa vb. elemanı olduğu görülmüştür. Estetik emeğin aranan önemli özelliklerden biri olduğu ortaya çıkmıştır. Kadın adayların tam zamanlı çalışma şeklinde istihdam edileceği tespit edilmiştir. Lise mezunu kadınların başvurabileceği daha çok ilanın olduğu gözlenmiştir. Çalışmada ayrıca dişil/eril özellikler ile eğitim seviyesi, pozisyon, departman ve sektör karşılaştırma sonuçlarına yer verilmiştir.
Osmanlı Kadın Tarihi Çalışmalarında Kullanılabilecek Birincil Kaynaklar | Primary Sources that can be Employed in the Field of History of Ottoman Women
Authors: İPŞİRLİ ARGIT Betül
Published: November 2022

Bu makale, Osmanlı kadın tarihi çalışmalarında kullanılabilecek birincil kaynaklar üzerinedir. Çalışmanın amacı asırlar boyunca geniş bir coğrafyada yaşamış, farklı din, ırk, etnik yapı, sosyoekonomik statüye mensup Osmanlı kadınlarının yaşamlarını inşa ederken kullanılabilecek kaynakları tanıtmak ve değerlendirmektir. Mevcut kaynaklar genel olarak yazılı kaynaklar, görsel kaynaklar, maddi kültür unsurları, mimari yapılar, mezar taşları ve sözlü kaynaklar olmak üzere belli kategoriler altında değerlendirilecektir. Ağırlıklı olarak 19. yüzyıl öncesi kaynaklar ve Osmanlılar tarafından üretilen kaynaklar üzerine durulacaktır. Osmanlı kadın tarihi çalışmalarında kullanılabilecek kaynakların çeşitliliğini göstermek ve muhtelif kategorideki kaynakların kadın tarihi alanında kaynak olarak değerine ışık tutmak amacında olan çalışmada, kaynakların genel olarak mahiyeti, hangi grup Osmanlı kadınları hakkında açılım sağladığı ve hangi konularda ne tür bilgilere erişme imkânı verdiği konusuna temas edilecektir. Ayrıca kaynakların sınırlarına, problemli taraflarına ve ihtiyatla yaklaşılması gereken yönlerine yer yer değinilecektir. Kaynakların zenginliğine dair farkındalığın artmasının, yeni kaynak arayışları içinde olarak kaynakların çeşitlendirilmesinin ve kaynakların değeri ölçülüp birbirleriyle koordineli kullanarak kaynakların potansiyelini zorlamanın, alanda sağlayabileceği açılımlara işaret edilecektir.
The Effect Of PBL Model On Learning Outcomes Theme 5 Subtheme 3 Learning 1 In Grade 3
Authors: Khoirun Nisak Siti Nur , Rochmania Desty Dwi
Published: November 2022

This study aims to determine student learning outcomes of critical thinking before using the PBL model on theme 5 sub-theme 3 learning 1 in grade 3 SDN Gedangan Student learning outcomes in critical thinking after using the PBL model on theme 5 sub-theme 3 learning 1 in grade 3 SDN Gedangan 1, and To find out the response after learning to think critically using the PBL model on theme 5 sub-theme 3 learning 1 in grade 3 SDN Gedangan 1. The results of this study are expected to provide ideas that can be used as a source of information to increase knowledge, as well as input and consideration for institutions that related to the PBL learning model. The design of this study was pre-experimental and the design used was a one group pretest-posttest design. The research subjects were 3rd grade students of SDN Gedaangan 1 Sumobito Jombang for the academic year 2021/2022. Data collection techniques using tests. The study was conducted in the even semester of January 2022. Students were given pre-test and before learning using the PBL learning model in learning theme 5 sub-theme 3 learning 1 in grade 3 SDN Gedangan 1, from the pretest and posttest tests carried out, the average pretest result was 62 with 18 students scored below the KKM and 16 students scored above the KKM, while the posttest average was 90, none of the students scored below the KKM. The positive response of student questionnaire data is 68.6%. These results indicate that the use of the PBL model in learning theme 5 sub-theme 3 learning 1 in class III SDN Gedangan 1 can have a positive effect on student learning outcomes and interest in learning.

Keywords: PBL Model, Critical Thinking Learning Outcomes
Citizenship in comparative perspective: populism in Rio de Janeiro and in Quebec
Authors: Monteiro Eduardo
Published: November 2022

Populists (re)orient social identities by creating a dichotomous perspective in which certain groups are selected to be a menace. How is this phenomenon possible in so different contexts? A cultural and an identity turn seems to be a necessary condition for the success of populism. This phenomenon is better seen at how candidates consider racial groups as ideal citizens and other as subcitizens. Critical Discourse Analysis helped to elucidate social-hierarchies and power relations towards racialized groups in Quebec and in Rio de Janeiro during 2018 elections. This framework deepens our comprehension on the complex causality of populism by focusing on citizenship.
Trends in research on the perioperative use of acupuncture in the last 20 years: A CiteSpace-based bibliometric analysis and literature review
Authors: Zhang Minjuan , Cairen Zhuoma , Zhang Junbao , Wang Yun , Lu Zhihong
Published: November 2022

Objective

Research on acupuncture-related techniques in the perioperative period has been increasing in the last 20 years. In this research, a bibliometric analysis was performed to review the application of acupuncture for perioperative medicine and to evaluate the trends in research on the perioperative use of acupuncture.

Methods

CiteSpace was used to analyze published papers on the perioperative use of acupuncture in the Web of Science database from 2001 to 2021. The research trends were evaluated based on the number of publications, keywords, journals, countries, institutions, and authors. Moreover, collaborative networks and reference co-citation network maps were analyzed.

Results

A total of 719 related studies were included in the analyses. The number of annual publications has gradually been increasing. Evidence-Based Complementary and Alternative Medicine published the most (49 articles). Regarding the cited journals, Anesthesia & Analgesia ranked first in terms of frequency (361), while Pain ranked first in terms of centrality (0.71). China (284) and the China Medical University (26) published the most. Wang Yu (21 articles) was the most active author, while Lee A (116) was the most co-cited author. The most co-cited reference was a meta-analysis of acupuncture for post-operative pain (212). The “post-operative cognitive dysfunction” cluster was new research directions that had recently emerged. Pain-related keywords had the highest frequency in keywords about indications.

Conclusion

Research on perioperative acupuncture is a promising field, and more cooperation in global scientific research is needed. Our results may help researchers in identifying potential collaborators, research frontiers, and hot topics.
Myocardial protection with glucose-insulin potassium in patients with acute coronary syndromes requiring coronary artery bypass grafting: A case series
Authors: Ellenberger Christoph , Hagerman Andres , Putzu Alessandro , Cikirikcioglu Mustafa , Licker Marc
Published: November 2022

Emergent cardiac surgery in patients with acute coronary syndrome (ACS) is always a challenging task for surgeons, anesthesiologists and patients. As “time is muscle,” early revascularization by percutaneous coronary intervention (PCI) has been largely advocated to salvage myocardial ischemic cells. In cases judged not suitable for PCI, on-pump coronary artery bypass grafting (CABG) is indicated with cardioplegic arrest and eventually anesthetic preconditioning to enhance myocardial protection. In these high-risk emergent procedures, adjuvant interventions to cardioplegic arrest are aimed to maximize the chance of cardiac functional recovery. Although the infusion of glucose-insulin-potassium (GIK) has demonstrated interesting cardioprotective effects in animal models of myocardial ischemia and in patients undergoing elective cardiac surgery, this cardioprotective strategy has not yet been adopted largely and has been ignored so far in emergent myocardial revascularization procedures. In this case series, we describe the effects of GIK on left ventricular performance assessed by transesophageal echocardiography in four patients with ACS who required emergent CABG surgery. The GIK solution of 20 g glucose, 20 UI insulin and 10 mEq potassium chloride was infused twice over 30 min, first after anesthesia induction and later after aortic unclamping. The systolic performance was assessed after anesthesia induction and after each GIK infusion using the 3D left ventricular ejection fraction, as well as the global longitudinal and circumferential strain. The diastolic function was assessed based on mitral inflow patterns (E-and A-waves) as well as flow propagation velocity.
Case report: Nasal polyp exfoliated into the tracheal tube in a patient with a difficult airway during nasotracheal intubation
Authors: Bai Fu-Rong , Zhang Shuang , Yi Ming-liang
Published: November 2022

Background

Difficult airway has always been one of the greatest challenges for anesthesiologists. Patients with difficult airways and severe obstructive sleep apnea-hypopnea syndrome (OSAHS) often find themselves in life-threatening danger before their artificial airway is established and after their artificial airway is removed.

Case presentation

The study reported a case of nasal polyps shedding into the tracheal tube when nasotracheal intubation in a patient diagnosed with a difficult airway and OSAHS; the patient was being prepared for a transoral tonsillectomy under general anesthesia. The uniqueness of this case was that nasal polyps were scraped off using the tracheal tube during nasotracheal intubation, and detected in real-time; this ultimately prevented an airway foreign body obstruction. Reviewing the literature on intubation methods for difficult airways, awake endotracheal intubation guided by fiberoptic bronchoscopy remains to be the gold standard for airway management. Additionally, nasotracheal intubation can provide more operating space for oral surgery. In this case, the unexpected nasal polyp fell off into the tracheal tube during intubation, as is visualized on the following pictures. This fortunate event prevented the nasal polyp from falling into the trachea and bronchus.

Conclusion

It is necessary to rule out any nasal abnormalities in patients requiring nasotracheal intubation; otherwise, blind nasal intubation may be dangerous.
An advanced and efficient asymmetric PCR method for microarray applications
Authors: Reddy Banda Suresh , Klapproth Holger , Smit Nicolaas , Bednar Sonja , Brandstetter Thomas , Rühe Jürgen
Published: November 2022

The sensitivity of a PCR based biochip assay relies on the efficiency of PCR amplicons in binding to the microarray spots. The essential factor determining the sensitivity is the amount of single stranded (ss) amplicons available for biochip hybridization. Asymmetric PCR can generate ss-amplicons depending on the ratio of primers used in the amplification process, but this process is often inefficient. We report a novel variant of PCR called the Asymmetric Exponential and Linear Amplification (AELA) which can overcome these issues and generate large amounts of single stranded amplicons. AELA-PCR introduces an amplification strategy that makes use of both exponential and linear amplification of the target nucleic acid. This is done by specifically designed primers and choice of adequate thermal profiles. In conventional PCR with a classical thermal profile, these specifically designed primers will work normally and contribute to an exponential increase of amplicons. A designed sequence extension of one of the primers and a very specific thermal profile, will result in a situation that the extended primer will be the only functional one for amplification, resulting in a linear phase of the amplification process. That is why during this step only one of the two strands of the target is amplified linearly and no longer exponentially. The result of the whole process is an amplification product enriched very strongly in one of the two single strands of the target. These adaptions in PCR are particularly favorable where the generation of ss-DNA/RNA is required. We demonstrate the higher biochip sensitivity of AELA-PCR compared to conventional amplification methods with an example of the Staphylococcus aureus detection on a DNA oligonucleotide microarray.
A review of technologies for direct lithium extraction from low Li+ concentration aqueous solutions
Authors: Murphy Olivia , Haji Maha N.
Published: November 2022

Under the Paris Agreement, established by the United Nations Framework Convention on Climate Change, many countries have agreed to transition their energy sources and technologies to reduce greenhouse gas emissions to levels concordant with the 1.5°C warming goal. Lithium (Li) is critical to this transition due to its use in nuclear fusion as well as in rechargeable lithium-ion batteries used for energy storage for electric vehicles and renewable energy harvesting systems. As a result, the global demand for Li is expected to reach 5.11 Mt by 2050. At this consumption rate, the Li reserves on land are expected to be depleted by 2080. In addition to spodumene and lepidolite ores, Li is present in seawater, and salt-lake brines as dissolved Li+ ions. Li recovery from aqueous solutions such as these are a potential solution to limited terrestrial reserves. The present work reviews the advantages and challenges of a variety of technologies for Li recovery from aqueous solutions, including precipitants, solvent extractants, Li-ion sieves, Li-ion-imprinted membranes, battery-based electrochemical systems, and electro-membrane-based electrochemical systems. The techno-economic feasibility and key performance parameters of each technology, such as the Li+ capacity, selectivity, separation efficiency, recovery, regeneration, cyclical stability, thermal stability, environmental durability, product quality, extraction time, and energy consumption are highlighted when available. Excluding precipitation and solvent extraction, these technologies demonstrate a high potential for sustainable Li+ extraction from low Li+ concentration aqueous solutions or seawater. However, further research and development will be required to scale these technologies from benchtop experiments to industrial applications. The development of optimized materials and synthesis methods that improve the Li+ selectivity, separation efficiency, chemical stability, lifetime, and Li+ recovery should be prioritized. Additionally, techno-economic and life cycle analyses are needed for a more critical evaluation of these extraction technologies for large-scale Li production. Such assessments will further elucidate the climate impact, energy demand, capital costs, operational costs, productivity, potential return on investment, and other key feasibility factors. It is anticipated that this review will provide a solid foundation for future research commercialization efforts to sustainably meet the growing demand for Li as the world transitions to clean energy.
The investigation of the NH3-SCR performance of a copper-based AEI-CHA intergrown zeolite catalyst
Authors: Ye Hongling , Ren Kai , Wang Pengfei , Wang Lin
Published: November 2022

This work prepared an ISAPO-34/SAPO-18 intergrown zeolite using phosphate organoamine as the structure guiding agent. Physical-chemical characterizations by XRD, SEM, TG, and BET showed that the SAPO-34/SAPO-18 presents a cross-stacked cubic block-like microscopic morphology, with characteristic diffusive diffraction peaks at 2θ = 16–18° and 30–33° and a specific surface area of 557 m2 g−1. The series of copper-based catalysts prepared from SAPO-34/SAPO-18 showed a shift of the active temperature window to a lower temperature with increasing copper content. Moreover, the Brønsted acid site decreased significantly due to copper ion exchange and zeolite structure framework damage. Among them, the 1.2 wt% sample showed the widest active temperature window, with a T90 range of 175–435°C. After low-temperature hydrothermal aging treatment, the zeolite structure was eroded and the catalyst activity deteriorated significantly.
3D printed cylindrical capsules as a Chlorella pyrenoidosa immobilization device for removal of lead ions contamination
Authors: Lan Shuzhen , Xia Xinshu , Liu Zhen , Yang Yujin , Qian Qingrong , Luo Yongjin , Chen Qinghua , Cao Changlin , Xiao Liren
Published: November 2022

Immobilization is considered as a promising strategy toward the practical applications of powdered adsorbent. Herein, three dimensional (3D) printing cylindrical capsules with cross-linked PVA hydrogels membrane in encapsulate Chlorella pyrenoidosa (Cp) were utilized for removal of lead ions. The chemical compositions, hydrogels performance and morphologies of the membranes were determined by Fourier transformed infrared spectroscopy (FTIR), cross-linking degree, swelling degree, membrane flux and scanning electron microscopy (SEM). It is found that PVA cross-linking structure is successfully synthesized on the surface of capsule body and cap due to the presence of PVA in the filament. The lead ions adsorption capacity related to initial concentration of 50 mg/L in 48 h is reached 75.61%, revealing a good removal ability. The self-floating 3D printed capsules device also shows an excellent recovering property. After 7 runs of adsorption experiment, the lead ions adsorption ratio remains 78.56%, which will bring a broad prospect in wastewater treatment, chemical slow release along with sample preparation and separation.
Wolbachia infection in field-collected Aedes aegypti in Yunnan Province, southwestern China
Authors: Zhang HengDuan , Gao Jian , Ma Zu , Liu Yuan , Wang Ge , Liu Qing , Du YuTong , Xing Dan , Li ChunXiao , Zhao Teng , Jiang YuTing , Dong YanDe , Guo XiaoXia , Zhao TongYan
Published: November 2022

Background

Wolbachia is gram-negative and common intracellular bacteria, which is maternally inherited endosymbionts and could expand their propagation in host populations by means of various manipulations. Recent reports reveal the natural infection of Wolbachia in Aedes Aegypti in Malaysia, India, Philippines, Thailand and the United States. At present, none of Wolbachia natural infection in Ae. aegypti has been reported in China.

Methods

A total of 480 Ae. aegypti adult mosquitoes were collected from October and November 2018 based on the results of previous investigations and the distribution of Ae. aegypti in Yunnan. Each individual sample was processed and screened for the presence of Wolbachia by PCR with wsp primers. Phylogenetic trees for the wsp gene was constructed using the neighbour-joining method with 1,000 bootstrap replicates, and the p-distance distribution model of molecular evolution was applied.

Results

24 individual adult mosquito samples and 10 sample sites were positive for Wolbachia infection. The Wolbachia infection rate (IR) of each population ranged from 0 - 41.7%. The infection rate of group A alone was 0%-10%, the infection rate of group B alone was 0%-7.7%, and the infection rate of co-infection with A and B was 0-33.3%.

Conclusions

Wolbachia infection in wild Ae. aegypti in China is the first report based on PCR amplification of the Wolbachia wsp gene. The Wolbachia infection is 5%, and the wAlbA and wAlbB strains were found to be prevalent in the natural population of Ae. aegypti in Yunnan Province.
“The whole is greater than the sum of its parts” – Exploring teachers’ technology readiness profiles and its relation to their emotional state during COVID-19 emergency remote teaching
Authors: Pozas Marcela , Letzel-Alt Verena , Schneider Christoph
Published: November 2022

With the rapid spread of the Coronavirus (COVID-19), schools around the world came to a shutdown. This resulted in an abrupt transition from face-to-face instruction to emergency remote teaching (ERT), resulting in numerous challenges that have greatly affected teachers. Even though research has identified key factors for teachers to effectively implement ERT in the context of the COVID-19 school shutdown, there is still little research on the factors (and their interrelations) that account for the differences between teachers’ experiences. Following a theoretical model of technology readiness, this study aimed to investigate teacher profiles based on domains of technology acceptance, technology competence and technology control. In addition, this study seeks to explore whether teachers’ emotional state during ERT varies between the teacher technology readiness profiles. A total of 124 teachers participated voluntarily in an online survey stemming from the research project “Students-Parents-Teachers in Homeschooling” in Germany. Results from a two-step cluster analysis revealed three distinct teacher technology readiness profiles. Moreover, the findings also revealed gender differences between the three technology readiness profiles. Lastly, an analysis of variance indicated that teachers’ positive emotional state during ERT varied significantly across the clusters. Implications of the results for teacher education and teacher professional development, as well as further lines of research are discussed.
Coagulation- and fibrinolysis-related genes for predicting survival and immunotherapy efficacy in colorectal cancer
Authors: Ma Yanling , Wang Bofang , He Puyi , Qi Wenbo , Xiang Ling , Maswikiti Ewetse Paul , Chen Hao
Published: November 2022

Background

Colorectal cancer (CRC) is a common cancer and has a poor prognosis. The coagulation system and fibrinolysis system are closely related to the progression of malignant tumors and is also related to the immunotherapy of malignant tumors. Herein, we tried to predict survival and the immunotherapy effect for patients with CRC using a novel potential prognostic model.

Methods

Through online data of TCGA and GEO, we screened significantly differentially expressed genes (DEGs) to construct a prognostic model, followed by obtaining immune-related genes (IRGs) from the ImmPort database and coagulation- and fibrinolysis-related genes (CFRGs) from the GeneCards database. The predictive power of the model is assessed by Kaplan–Meier survival curves as well as the time-dependent ROC curve. Moreover, univariate and multivariate analyses were conducted for OS using Cox regression models, and the nomogram prognostic model was built. In the end, we further studied the possibility that CXCL8 was associated with immunocyte infiltration or immunotherapy effect and identified it by immunohistochemistry and Western blot.

Results

Five DEGs (CXCL8, MMP12, GDF15, SPP1, and NR3C2) were identified as being prognostic for CRC and were selected to establish the prognostic model. Expression of these genes was confirmed in CRC samples using RT-qPCR. Notably, those selected genes, both CFRGs and IRGs, can accurately predict the OS of CRC patients. Furthermore, CXCL8 is highly correlated with the tumor microenvironment and immunotherapy response in CRC.

Conclusion

Overall, our established IRGPI can very accurately predict the OS of CRC patients. CXCL8 reflects the immune microenvironment and reveals the correlation with immune checkpoints among CRC patients.
A highly sensitive bead-based flow cytometric competitive binding assay to detect SARS-CoV-2 neutralizing antibody activity
Authors: Yao Xiangyu , Zhang Zhichao , Mei Qingmin , Li Shenwei , Xing Li , Long Yali , Zhang Demei , Wang Jing , Wang Xiedong , Xie Bin , Yang Bo , Gao Yong , Wu Changxin , Meng Qinglai
Published: November 2022

Accurate detection of SARS-CoV-2 neutralizing antibody (nAb) is critical for assessing the immunity levels after virus infection or vaccination. As fast, cost-effective alternatives to viral infection-based assays, competitive binding (CB) assays were developed to quantitate nAb by monitoring the ability of sera to inhibit the binding of viral spike (S) protein to the angiotensin converting enzyme 2 (ACE2) receptor. Herein, we established a bead-based flow cytometric CB assay and tested the detection performance of six combination models, i.e. immobilized ACE2 and soluble Fc-tagged S1 subunit of S protein (iACE2/S1-Fc), immobilized ACE2 and soluble Fc-tagged receptor binding domain (RBD) of S protein (iACE2/RBD-Fc), immobilized S1 and soluble Fc-tagged ACE2 (iS1/ACE2-Fc), immobilized S1 and soluble His-tagged ACE2 (iS1/ACE2-His), immobilized RBD and soluble Fc-tagged ACE2 (iRBD/ACE2-Fc), and immobilized RBD and soluble His-tagged ACE2 (iRBD/ACE2-His). Using SARS-CoV-2 monoclonal antibodies and sera of convalescent COVID-19 patients and vaccinated subjects, the combination models iACE2/RBD-Fc, iACE2/S1-Fc and iS1/ACE2-His were identified to be able to specifically detect SARS-CoV-2 nAb, among which iACE2/RBD-Fc model showed the highest sensitivity, superior to a commercial SARS-CoV-2 surrogate virus neutralization test (sVNT) ELISA kit. Further studies demonstrated that the sensitivity and specificity of CB assays were affected by the tag of ACE2, type of spike and method of measuring binding rate between ACE2 and spike. Moreover, the iACE2/RBD-Fc model showed good performance in detecting kinetic development of nAb against both the prototype SARS-CoV-2 strain and an omicron variant of SARS-CoV-2 in people immunized by an inactivated SARS-CoV-2 vaccine, and the results of iACE2/RBD-Fc model are correlated well with those of live virus-based and pseudovirus-based neutralization tests, demonstrating the potential to be developed into a highly sensitive, specific, versatile and high-throughput method for detecting SARS-CoV-2 nAb in clinical practice.
Cortisol awake response imbalance as an indicator of acute central serous chorioretinopathy: Relationship with choriocapillaris and choroidal features
Authors: Scarinci Fabio , Patacchioli Francesca Romana , Costanzo Eliana , Parravano Mariacristina
Published: November 2022

Purpose

The purpose of the present study was to measure in central serous chorioretinopathy (CSC) the salivary cortisol awake response (CAR) delta percentage (Δ%) variation, a distinct and robust indicator of cortisol rhythm during wakefulness, commonly proposed as a marker of hypothalamic-pituitary adrenal (HPA) axis activity, whose alteration is frequently associated with several adverse health outcomes.

Methods

In the present cross-sectional observational study, salivary CAR Δ% variation was assessed in 17 adult male subjects affected by acute naïve CSC and compared to 17 matched healthy controls. Choroid vasculature metrics were assessed in the study population by measuring the subfoveal choroidal thickness (FCT) and the choroidal vascularity index (CVI) by the imaging technique of enhanced-depth imaging spectral-domain optical coherence tomography (EDI-SD-OCT). Furthermore, flow signal void area features of the choriocapillaris were evaluated in the study population using OCT angiography (OCTA).

Results

Both the control and CSC groups showed a physiological cortisol increase that occurred during the first 30 min after awaking. However, CSC adult male patients showed remarkably blunted CAR Δ% variation in comparison with controls, which might reflect a CSC-related imbalance of HPA axis activity. Statistically significant correlations were shown by Pearson’s correlation test between salivary CAR Δ% and the selected choroidal and choriocapillaris imaging biomarkers (FCT, CVI, and flow signal void area) in the study population.

Conclusion

In conclusion, alterations of the CAR Δ% increase, associated with choroidal-retinal metrics, might provide a window into the physiopathology of acute CSC, suggesting a possible common factor to explain the association between stress and CSC.
Response of soil microbial compositional and functional heterogeneity to grazing exclusion in alpine shrub and meadows in the Qinghai–Tibet Plateau
Authors: Wang Shilin , Abalori Theophilus Atio , Wang Wenhu , Deng Xiuxia , Liu Wanting , Wang Jinlan , Cao Wenxia
Published: November 2022

Soil microorganisms found in shrub-meadow ecosystems are highly heterogeneous and extremely sensitive to grazing, but changes in microbial compositional and functional heterogeneity during grazing exclusion (GE) have been largely overlooked compared to community diversity. We collected soil samples from heavily grazed plots (6.0 sheep/ha) and GE plots (matrix and patch areas in both), and used a combination of next-generation sequencing, vegetation features, and the associated soil property data to investigate the effect of GE on the composition and function of microbial communities (bacteria fungi, and archaea) in 0–10 cm soils. Regarding community composition, the proportions of species in bacteria, fungi, and archaea were 97.3, 2.3, and 0.4%, respectively. GE significantly affected the species diversity of fungi and archaea but not that of bacteria. GE decreased the heterogeneity of bacteria (2.9% in matrix and 6.2% in patch) and archaea (31.1% in matrix and 19.7% in patch) but increased that of fungi by 1.4% in patch. Regarding community function, enzyme diversity and heterogeneity were increased by 10.4 and 9.4%, respectively, in patch after 6 years of fencing, exemplifying a high level of microbial functional redundancy. The Kyoto Encyclopedia of Genes and Genome pathways—cell growth and death, translation, digestive system, and nucleotide metabolism—were functional biomarkers (linear discriminant analysis effect size method) in matrix-non-grazed plots, whereas lipid metabolism, xenobiotics biodegradation and metabolism, and metabolism of terpenoids and polyketides, cell motility, cancer: overview, endocrine system, and membrane transport were biomarkers in patch-non-grazed plots. Additionally, GE improved the capacity for fatty acid metabolism but decreased the abundance of methane-producing archaea by 42.9%. Redundancy analysis revealed that the factors that affected microbial composition the most were soil aggregates, soil moisture, and the number of plant species, whereas those that affected microbial function the most were soil available phosphorus, soil temperature, and shrub canopy diameter. Our results quantified soil microbial heterogeneity, emphasizing the different responses of the composition and function of bacteria, fungi, and archaea to GE in alpine shrubs and meadows.
The repertoire of protein-sulfatide interactions reveal distinct modes of sulfatide recognition
Authors: Capelluto Daniel G. S
Published: November 2022

Sulfatide is an abundant glycosphingolipid in the mammalian nervous system, kidney, trachea, gastrointestinal tract, spleen, and pancreas and is found in low levels in other tissues. Sulfatide is characterized by the presence of a sulfate group in the hydrophilic galactose moiety, with isoforms differing in their sphingosine base and the length, unsaturation, and hydroxylation of their acyl chain. Sulfatide has been associated with a variety of cellular processes including immune responses, cell survival, myelin organization, platelet aggregation, and host-pathogen interactions. Structural studies of protein-sulfatide interactions markedly advanced our understanding of their molecular contacts, key-interacting residues, orientation of the sulfatide in its binding site, and in some cases, sulfatide-mediated protein oligomerization. To date, all protein-sulfatide interactions are reported to display dissociation constants in the low micromolar range. At least three distinct modes of protein-sulfatide binding were identified: 1) protein binding to short consensus stretches of amino acids that adopt α-helical-loop-α-helical conformations; 2) sulfatide-bound proteins that present the sulfatide head group to another protein; and 3) proteins that cage sulfatides. The scope of this review is to present an up-to-date overview of these molecular mechanisms of sulfatide recognition to better understand the role of this glycosphingolipid in physiological and pathological states.