bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Mixing Relations for Determining the Effective Thermal Conductivity of Open-Cell Foams
Authors: Camacho Hernandez Jesus Nain , Link Guido , Schubert Markus , Hampel Uwe
Published: March 2022

This paper proposes a new approach to relate the effective thermal conductivity of open-cell solid foams to their porosity. It is based on a recently published approach for estimating the dielectric permittivity of isotropic porous media. A comprehensive assessment was performed comparing the proposed mixing relation with published experimental data for thermal conductivity and with numerical data from state-of-the-art relations. The mixing relation for the estimation of thermal conductivities based on dodecahedrons as building blocks shows good agreement with experimental data over a wide range of porosity.
Colour Change of Sustainable Concrete Containing Waste Ceramic and Hybrid Fibre: Effect of Temperature
Authors: Najm Hadee Mohammed , Nanayakkara Ominda , Ahmad Mahmood , Sabri Sabri Mohanad Muayad
Published: March 2022

Construction and demolition (C&D) waste was considered as zero value materials and, as a result, most C&D waste materials ended up in landfills, eventually constituting environmental issues. Therefore, it is important to explore the potential of such C&D waste materials for concrete production. Thus, this research effort aims to find a new method to improve sustainable concrete properties exposed to elevated temperatures at the lowest costs and identify the relationship between temperature change and a change in colour in a heat-exposed concrete structure. Therefore, this study explored the behaviour of three types of concrete: plain concrete (PC), waste ceramic optimal concrete (WOC), and waste ceramic optimal concrete reinforced by hybrid fibre (WOC-Hybrid) in ambient and elevated temperature. The study shows that colour change in a concrete structure exposed to high temperature has a consistent relationship, where it has been found that the colour alteration is of great interest because its appearance usually coincides with the onset of a significant loss of concrete strength as a result of elected temperatures. Overall, it can be considered that waste ceramic materials reinforced by hybrid fibres can be used for concrete production, and by doing so, the negative impact of these wastes on the environment can be controlled as well as fortify the mechanical properties.
Structure and Photoluminescence Properties of Dy3+ Doped Phosphor with Whitlockite Structure
Authors: Tang Wanjuan , Guo Qingfeng , Su Ke , Liu Haikun , Zhang Yuanyuan , Mei Lefu , Liao Libing
Published: March 2022

Whitlockite has the advantages of a low sintering temperature, high stability, and a low fabrication cost, and it is widely used as the host for luminescent material. In this study, Ca1.8Li0.6La0.6−x(PO4)2:xDy3+ phosphor was prepared by the high-temperature solid-state method, and its structure, composition, and luminescence properties were systematically studied. The results showed that a new whitlockite type matrix was prepared by replacing Ca2+ in whitlockite with monovalent and trivalent cations. The prepared phosphors belonged to a hexagonal crystal system with a particle size in the range of 5–20 μm. Under the excitation of 350 nm UV light, the samples emitted white light, and there were mainly two stronger emission peaks at 481 nm in the blue band and 573 nm in the yellow band, which correspond to the electron transitions at 4F9/2→6H15/2 and 4F9/2→6H13/2 of Dy3+, respectively. The optimal doping concentration of Dy3+ in Ca1.8Li0.6La0.6(PO4)2 matrix was 0.03 (mol%). The main mechanism of concentration quenching in the sample was dipole–dipole energy transfer. When the temperature was 130 °C, the luminescence intensity of the samples was 78.7% of that at 30 °C, and their thermal quenching activation energy was 0.25 eV. The CIE coordinates of the sample at 30 °C were (0.2750, 0.3006), and their luminescent colors do not change with temperature. All the results indicate that Ca1.8Li0.6La0.6−x(PO4)2:xDy3+ phosphor is a luminescent material with good luminescence performance and thermal stability, which shows a promising application in the field of LED display.
0D/2D Mixed Dimensional Lead-Free Caesium Bismuth Iodide Perovskite for Solar Cell Application
Authors: Masawa Salma Maneno , Li Jihong , Zhao Chenxu , Liu Xiaolong , Yao Jianxi
Published: March 2022

Bismuth-based perovskites are potentially a promising alternative for lead-free perovskites. During bond formation, however, trivalent ions on Cs3Bi2I9 with CsI/BiI3 ratio of 1.5/1 form 0D-neutral charged compounds with higher bandgap (>2.0 eV) and poor absorption capacity. Mixed 0/2-dimensional structures are potentially suitable substitutes due to their low bandgap. So far, the reported CsI/BiI3 ratios for 0D/2D structures are 1:1, 1:2 and 1:3. Herein, a new ratio of 1/1.5 is reported. Caesium bismuth iodide at a ratio of CsI/BiI3 of 1/1.5 was synthesised using a one-step processing method with/without solvent vapour annealing. During solvent annealing, a 1/4 (v/v) mixture of DMF/methanol was used as a solvent. The crystal structure formed at a ratio of 1/1.5 is more similar to 1.5/1 than to 1/3. The XRD pattern revealed additional characteristics peaks at 009, 012, 209 and 300, indicating the growth of another phase. The formed heterogeneous mixed 0D/2D structure has an extended light absorption capacity greater than 720 nm. Solvent vapour annealing improved film morphology by enhancing grain size and packing density. When cells with and without solvent vapour annealing are compared, the power conversion efficiency of caesium bismuth iodide increases from 0.26% without solvent annealing to 0.98% with solvent vapour annealing. This study establishes a new route for future research on crystal configuration, nomenclature, film and morphology, quality tailoring and applications toward the goal of lead-free perovskite solar cells.
Numerical Simulation and Analysis of the Flow Characteristics of the Roof-Attached Vortex (RAV) in a Closed Pump Sump
Authors: Zhang Bowen , Cheng Li , Zhu Minghu , Jiao Weixuan , Zhang Di
Published: March 2022

Unsteady numerical simulation and visual experiment are used to reveal the formation mechanism of the roof-attached vortex (RAV) on the roof of the closed sump of a pumping station. The results show that RAVs mainly occur between the pump device and the rear wall of the closed sump. In the 10th period of impeller rotation, there are 2 RAVs at the roof. V1 (Vortex 1 in numerical simulation) is located directly behind the water pump unit, and V2 (Vortex 2 in numerical simulation) is close to the right wall. Significantly, the vorticity intensity at the V1 vortex core increases with the rotation of the impeller. Vtest1 (Vortex 1 in test) and Vtest2 (Vortex 2 in test) are two RAVs observed in the experiment, which are highly consistent with the unsteady numerical simulation V1 and V2. Comparing the vorticity intensity of the roof, rear wall, and sidewall, it can be seen that the maximum vorticity intensity on the roof is more significant than that on the rear wall and both sides of the wall. The roof is more likely to induce vortex. When the RAVs on the roof occur, the pressure in the middle of the bell mouth is lower than that on the sidewall, and the velocity is higher. At 2/5 T, the blade is in the low-pressure zone. The velocity distribution uniformity and velocity weighted average angle at the bell mouth also decreased. The RAVs enter the pump after being generated, which is the most harmful to the safe and stable operation of the pump. The study can provide theoretical guidance for the optimal design of the closed sump.
Concurrent Control Chart Pattern Recognition: A Systematic Review
Authors: García Ethel , Peñabaena-Niebles Rita , Jubiz-Diaz Maria , Perez-Tafur Angie
Published: March 2022

The application of statistical methods to monitor a process is critical to ensure its stability. Statistical process control aims to detect and identify abnormal patterns that disrupt the natural behaviour of a process. Most studies in the literature are focused on recognising single abnormal patterns. However, in many industrial processes, more than one unusual control chart pattern may appear simultaneously, i.e., concurrent control chart patterns (CCP). Therefore, this paper aims to present a classification framework based on categories to systematically organise and analyse the existing literature regarding concurrent CCP recognition to provide a concise summary of the developments performed so far and a helpful guide for future research. The search only included journal articles and proceedings in the area. The literature search was conducted using Web of Science and Scopus databases. As a result, 41 studies were considered for the proposed classification scheme. It consists of categories designed to assure an in-depth analysis of the most relevant topics in this research area. Results concluded a lack of research in this research field. The main findings include the use of machine learning methods; the study of non-normally distributed processes; and the consideration of abnormal patterns different from the shift, trend, and cycle behaviours.
Mond-Weir and Wolfe Duality of Set-Valued Fractional Minimax Problems in Terms of Contingent Epi-Derivative of Second-Order
Authors: Das Koushik , Treanţă Savin , Saeed Tareq
Published: March 2022

This paper is devoted to provide sufficient Karush Kuhn Tucker (in short, KKT) conditions of optimality of second-order for a set-valued fractional minimax problem. In addition, we define duals of the types Mond-Weir and Wolfe of second-order for the problem. Further we obtain the theorems of duality under contingent epi-derivative together with generalized cone convexity suppositions of second-order.
Empirical Study of Data-Driven Evolutionary Algorithms in Noisy Environments
Authors: Lin Dalue , Huang Haogan , Li Xiaoyan , Gong Yuejiao
Published: March 2022

For computationally intensive problems, data-driven evolutionary algorithms (DDEAs) are advantageous for low computational budgets because they build surrogate models based on historical data to approximate the expensive evaluation. Real-world optimization problems are highly susceptible to noisy data, but most of the existing DDEAs are developed and tested on ideal and clean environments; hence, their performance is uncertain in practice. In order to discover how DDEAs are affected by noisy data, this paper empirically studied the performance of DDEAs in different noisy environments. To fulfill the research purpose, we implemented four representative DDEAs and tested them on common benchmark problems with noise simulations in a systematic manner. Specifically, the simulation of noisy environments considered different levels of noise intensity and probability. The experimental analysis revealed the association relationships among noisy environments, benchmark problems and the performance of DDEAs. The analysis showed that noise will generally cause deterioration of the DDEA’s performance in most cases, but the effects could vary with different types of problem landscapes and different designs of DDEAs.
Comparative Study of Polycaprolactone Electrospun Fibers and Casting Films Enriched with Carbon and Nitrogen Sources and Their Potential Use in Water Bioremediation
Authors: Pompa-Monroy Daniella Alejandra , Iglesias Ana Leticia , Dastager Syed Gulam , Thorat Meghana Namdeo , Olivas-Sarabia Amelia , Valdez-Castro Ricardo , Hurtado-Ayala Lilia Angélica , Cornejo-Bravo José Manuel , Pérez-González Graciela Lizeth , Villarreal-Gómez Luis Jesús
Published: March 2022

Augmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Furthermore, different types of carbon (glucose, fructose, lactose and galactose) and nitrogen sources (yeast extract, glycine, peptone and urea) were added to the scaffold to determinate their influence in bacterial growth. Bacterial growth was observed by scanning electron microscopy; thermal characteristics were also evaluated; bacterial cell growth was measured by ultraviolet-visible spectrophotometry at 600-nm. Fibers produced have an average diameter between 313 to 766 nm, with 44% superficial porosity of the scaffolds, a glass transition around ~64 °C and a critical temperature of ~338 °C. The fibrous scaffold increased the cell growth of Escherichia coli by 23% at 72 h, while Pseudomonas aeruginosa and Staphylococcus aureus increased by 36% and 95% respectively at 48 h, when compared to the normal growth of their respective bacterial cultures. However, no significant difference in bacterial growth between the scaffolds and the casted films could be observed. Cell growth depended on a combination of several factors: type of bacteria, carbon or nitrogen sources, casted films or 3D scaffolds. Microscopy showed traces of a biofilm formation around 3 h in culture of P. aeruginosa. Water bioremediation studies showed that P. aeruginosa on poly (caprolactone)/Glucose fibers was effective in removing 87% of chromium in 8 h.
Influence of Y Doping on WO3 Membranes Applied in Electrolyte-Insulator-Semiconductor Structures
Authors: Kao Chyuan-Haur , Liao Yu-Ching , Chuang Chi-Chih , Huang Yi-Hsuan , Lee Chang-Hsueh , Chen Shih-Ming , Lee Ming-Ling , Chen Hsiang
Published: March 2022

In this paper, tungsten oxide (WO3) is deposited on a silicon substrate applied in electrolyte-insulator-semiconductor structures for pH sensing devices. To boost the sensing performance, yttrium (Y) is doped into WO3 membranes, and annealing is incorporated in the fabrication process. To investigate the effects of Y doping and annealing, multiple material characterizations including X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), atom force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) are performed. Material analysis results indicate that annealing and Y doping can increase crystallinity, suppress defects, and enhance grainization, thereby strengthening membrane sensing capabilities in terms of sensitivity, linearity, and reliability. Because of their stable response, high reliability, and compact size, Y-doped WO3 membranes are promising for future biomedical applications.
Formation of Cells and Subgrains and Its Influence on Properties
Authors: Sandström Rolf
Published: March 2022

During plastic deformation, cells and subgrains are created in most alloys. This is collectively referred as the formation of a substructure. There is extensive qualitative information about substructures in the literature, but quantitative modeling has only appeared recently. In this paper, basic models for the formation of substructure during creep and deformation at constant strain rate are presented. It is demonstrated that the models can give at least an approximate description of available experimental data. The presence of substructure can have a dramatic impact on properties. It is well-known that prior cold work can significantly increase the creep strength. Cold work of copper can raise the creep rupture time by up to six orders of magnitude. During plastic deformation dislocations with opposite Burgers vectors move in different directions creating polarized or unbalanced dislocations. Since the unbalanced dislocations are not exposed to static recovery, they form a stable dislocation structure. Taking the role of the unbalanced dislocations into account, the full increase of the creep strength after cold work can quantitatively be explained (without the use of adjustable parameters). Additionally, the shape of the creep curves that varies with the amount of cold work can be modeled. The substructure is also of importance for the modeling of creep curves for material without cold work. In power-law breakdown, the stress exponent can be 50 or more. This should imply that there would be a huge increase in the creep rate with increasing strain, but that is not observed. The reason is that the unbalanced dislocations form a back stress that acts against the increase in the true stress. Taking the back stress into account, it has been possible to model creep curves for copper at near ambient temperatures. This effect must be taken into account in stress analysis to avoid overestimating the creep rate by many orders of magnitude.
The GUM Tree Calculator: A Python Package for Measurement Modelling and Data Processing with Automatic Evaluation of Uncertainty
Authors: Hall Blair D.
Published: March 2022

There is currently interest in the digitalisation of metrology because technologies that can measure, analyse, and make critical decisions autonomously are beginning to emerge. The notions of metrological traceability and measurement uncertainty should be supported, following the recommendations in the Guide to the Expression of Uncertainty in Measurement (GUM). However, GUM offers no specific guidance. Here, we report on a Python package that implements algorithmic data processing using ‘uncertain numbers’, which satisfy the general criteria in GUM for an ideal format to express uncertainty. An uncertain number can represent a physical quantity that has not been determined exactly. Using uncertain numbers, measurement models can be expressed clearly and succinctly in terms of the quantities involved. The algorithms and simple data structures we use provide an example of how metrological traceability can be supported in digital systems. In particular, uncertain numbers provide a format to capture and propagate detailed information about quantities that influence a measurement along the various stages of a traceability chain. More detailed information about influence quantities can be exploited to extract more value from results for users at the end of a traceability chain.
An Automated Power Evaluation Workbench for Triboelectric Nanogenerators
Authors: Yuan Ming , Li Chunhui , Zhang Sheng , Xie Yannan
Published: March 2022

Triboelectric nanogenerators (TENGs) have high potential in self-powered sensing and energy harvesting applications. In general, TENGs’ internal source resistance is high, and their output power varies under different load resistance values. Therefore, a resistance box is required to evaluate their energy harvesting performance and obtain the power curve under different load values. The load tuning process is usually performed by hand. This repetitive process is time-consuming and error-prone. Consequently, an Automated Power Evaluation Workbench (APEW) is developed, making the resistance switching and power measuring process program-controlled. The resistance value is resolved using the Octal decomposition principle. In addition, a resistance synthesis algorithm is proposed to alter the resistance value with a minimum step of 1 Ohm. The target resistance value is physically synthesized by relay switching, while digital lines control the relays. The proposed APEW is then evaluated experimentally, and the obtained results are compared with those of the traditional manual switching approach. It is deduced that the two power curves are almost identical. Therefore, it is believed that the proposed APEW will play a crucial role in TENG’s further development.
Cellular Responses Required for Oxidative Stress Tolerance of the Necrotrophic Fungus Alternaria alternata, Causal Agent of Pear Black Spot
Authors: Zhang Miao , Zhang Yandong , Li Yongcai , Bi Yang , Mao Renyan , Yang Yangyang , Jiang Qianqian , Prusky Dov
Published: March 2022

To establish successful infections in host plants, pathogenic fungi must sense and respond to an array of stresses, such as oxidative stress. In this study, we systematically analyzed the effects of 30 mM H2O2 treatment on reactive oxygen species (ROS) metabolism in Alternaria alternata. Results showed that 30 mM H2O2 treatment lead to increased O2− generation rate and H2O2 content, and simultaneously, increased the activities and transcript levels of NADPH oxidase (NOX). The activities and gene expression levels of enzymes related with ascorbic acid-glutathione cycle (AsA-GSH cycle) and thioredoxin systems, including superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (AXP) and thioredoxin (TrxR), were remarkably enhanced by 30 mM H2O2 stress treatment. Additionally, 30 mM H2O2 treatment decreased the glutathione (GSH) content, whereas it increased the amount of oxidized glutathione (GSSG), dehydroascorbate (DHA) and ascorbic acid (AsA). These results revealed that cellular responses are required for oxidative stress tolerance of the necrotrophic fungus A. alternata.
Cutaneous Complications of mRNA and AZD1222 COVID-19 Vaccines: A Worldwide Review
Authors: Kroumpouzos George , Paroikaki Maria Eleni , Yumeen Sara , Bhargava Shashank , Mylonakis Eleftherios
Published: March 2022

Because of the increasing emergence of cutaneous reactions from COVID-19 vaccines worldwide, we investigated the published reports of these complications. We searched the PubMed, Google Scholar, and Scopus databases and the preprint server bioRxiv for articles on cutaneous complications linked to mRNA-1273 (Moderna), BNT162b2 (Pfizer–BioNTech), and AZD1222 (AstraZeneca–Oxford University) vaccines published until 30 September 2021. Eighty studies describing a total of 1415 reactions were included. Cutaneous reactions were more prevalent in females (81.6%). Delayed large local reactions were the most common complication (40.4%), followed by local injection site reactions (16.5%), zoster (9.5%), and urticarial eruptions (9.0%). Injection site and delayed large local reactions were predominantly caused by the mRNA-1273 vaccine (79.5% and 72.0%, respectively). BNT162b2 vaccination was more closely linked to distant reactions (50.1%) than mRNA-1273 (30.0%). Zoster was the most common distant reaction. Of reactions with adequate information for both vaccine doses, 58.3% occurred after the first dose only, 26.9% after the second dose only, and 14.8% after both doses. Overall, a large spectrum of cutaneous reaction patterns occurred following the COVID-19 vaccination. Most were mild and without long-term health implications. Therefore, the occurrence of such dermatologic complications does not contraindicate subsequent vaccination.
Epitheliocystis in Greater Amberjack: Evidence of a Novel Causative Agent, Pathology, Immune Response and Epidemiological Findings
Authors: Cascarano Maria Chiara , Ruetten Maja , Vaughan Lloyd , Tsertou Maria Ioanna , Georgopoulou Dimitra , Keklikoglou Kleoniki , Papandroulakis Nikos , Katharios Pantelis
Published: March 2022

Epitheliocystis is a fish gill disease caused by a broad range of intracellular bacteria infecting freshwater and marine fish worldwide. Here we report the occurrence and progression of epitheliocystis in greater amberjack reared in Crete (Greece). The disease appears to be caused mainly by a novel Betaproteobacteria belonging to the Candidatus Ichthyocystis genus with a second agent genetically similar to Ca. Parilichlamydia carangidicola coinfecting the gills in some cases. After a first detection of the disease in 2017, we investigated epitheliocystis in the following year’s cohort of greater amberjack juveniles (cohort 2018) transferred from inland tanks to the same cage farm in the open sea where the first outbreak was detected. This cohort was monitored for over a year together with stocks of gilthead seabream and meagre co-farmed in the same area. Our observations showed that epitheliocystis could be detected in greater amberjack gills as early as a month following the transfer to sea cages, with ionocytes at the base of the gill lamellae being initially infected. Cyst formation appears to trigger a proliferative response, leading to the fusion of lamellae, impairment of gill functions and subsequently to mortality. Lesions are characterized by infiltration of immune cells, indicating activation of the innate immune response. At later stages of the outbreak, cysts were no longer found in ionocytes but were observed in mucocytes at the trailing edge of the filament. Whole cysts appeared finally to be expelled from infected mucocytes directly into the water, which might constitute a novel means of dispersion of the infectious agents. Molecular screening indicates that meagre is not affected by this disease and confirms the presence of previously described epitheliocystis agents, Ca. Ichthyocystis sparus, Ca. Ichthyocystis hellenicum and Ca. Similichlamydia spp., in gilthead seabream. Prevalence data show that the bacteria persist in both gilthead seabream and greater amberjack cohorts after first infection.
High-Resolution Topographic and Chemical Surface Imaging of Chalk for Oil Recovery Improvement Applications
Authors: Bredal Tine , Zimmermann Udo , Madland Merete , Minde Mona , Ost Alexander , Wirtz Tom , Audinot Jean-Nicolas , Korsnes Reidar
Published: March 2022

Chalk is a very fine-grained carbonate and can accommodate high porosity which is a key characteristic for high-quality hydrocarbon reservoirs. A standard procedure within Improved Oil Recovery (IOR) is seawater-injection which repressurizes the reservoir pore pressure. Long-term seawater-injection will influence mineralogical processes as dissolution and precipitation of secondary minerals. These secondary minerals (<1 micrometer) precipitate during flooding experiments mimicking reservoir conditions. Due to their small sizes, analysis from traditional scanning electron microscopy combined with energy dispersive X-ray spectroscopy is not conclusive because of insufficient spatial resolution and detection limit. Therefore, chalk was analyzed with high-resolution imaging by helium ion microscopy (HIM) combined with secondary ion mass spectrometry (SIMS) for the first time. Our aim was to identify mineral phases at sub-micrometer scale and identify locations of brine–rock interactions. In addition, we wanted to test if current understanding of these alteration processes can be improved with the combination of complementary imaging techniques and give new insights to IOR. The HIM-SIMS imaging revealed well-defined crystal boundaries and provided images of excellent lateral resolution, allowing for identification of specific mineral phases. Using this new methodology, we developed chemical identification of clay minerals and could define their exact location on micron-sized coccolith grains. This shows that it is essential to study mineralogical processes at nanometer scale in general, specifically in the research field of applied petroleum geology within IOR.
Meteoric 10Be, 137Cs and 239+240Pu as Tracers of Long- and Medium-Term Soil Erosion—A Review
Authors: Loba Aleksandra , Waroszewski Jarosław , Sykuła Marcin , Kabala Cezary , Egli Markus
Published: March 2022

Isotopes of meteoric 10Be, 137Cs, 239+240Pu have been proposed as a soil redistribution tracer and applied worldwide as an alternative method to classical field-related techniques (e.g., sediment traps). Meteoric 10Be provides information about long-term soil redistribution rates (millennia), while 137Cs and 239+240Pu give medium-term rates (decades). A significant progress in developing new models and approaches for the calculation of erosion rates has been made; thus, we provide a global review (n = 59) of research articles to present these three isotopes (meteoric 10Be, 239+240Pu and 137Cs) as soil erosion markers in different environments and under different land-use types. Understanding the dynamics and behaviours of isotopes in the soil environment is crucial to determine their usefulness as soil erosion tracers; thus, we discuss the chemical–physical behaviour of meteoric 10Be, 137Cs and 239+240Pu in soils. The application of these isotopes sometimes has strong limitations, and we give suggestions on how to overcome them or how to adapt them to a given situation. This review also shows where these isotopic methods can potentially be applied in the future. A lack in knowledge about soil redistribution rates exists particularly in loess-dominated areas where the tillage system has changed or in areas with strong wind erosion.
Alfalfa (Medicago sativa) Sprouts Respiratory Responses to Cadmium Stress Using IR LPAS
Authors: Popa Cristina , Petrus Mioara , Bratu Ana
Published: March 2022

Cadmium (Cd) is not considered a fundamental element for plants inducing general growth disturbances and inhibition in many species of plants. The purpose of our research was to examine the ethylene (C2H4) and ammonia (NH3), emissions in alfalfa sprouts with or without Cd, using infrared laser photoacoustic spectroscopy (IR LPAS), in order to suggest new markers that may add a better knowledge of Cd effect. The responses of alfalfa sprouts to C2H4 and NH3 may fluctuate, depending on tissue sensitivity and the phase of plant development. From the determinations of this study, the C2H4 was established to be inhibited, while NH3 was established to be in a higher concentration with the amount of Cd added to the alfalfa seeds for growth when the values were correlated to the control and BIOalfalfa sprouts (Sonnentor).
IAM Chromatographic Models of Skin Permeation
Authors: Sobańska Anna W. , Brzezińska Elżbieta
Published: March 2022

Chromatographic retention factor log kIAM obtained from IAM HPLC chromatography with buffered aqueous mobile phases and calculated molecular descriptors (surface area—Sa; molar volume—VM; polar surface area—PSA; count of freely rotable bonds—FRB; H-bond acceptor count—HA; energy of the highest occupied molecular orbital—EHOMO; energy of the lowest unoccupied orbital—ELUMO; and polarizability—α) obtained for a group of 160 structurally unrelated compounds were tested in order to generate useful models of solutes’ skin permeability coefficient log Kp. It was established that log kIAM obtained in the conditions described in this study is not sufficient as a sole predictor of the skin permeability coefficient. Simple put, potentially useful models based on log kIAM and readily available calculated descriptors, accounting for 85 to 91% of the total variability, were generated using Multiple Linear Regression (MLR).The models proposed in the study were tested on a group of 20 compounds with known experimental log Kp values.
Are Vitamin E Supplementation Beneficial for Female Gynaecology Health and Diseases?
Authors: Md Amin Nur Amira , Sheikh Abdul Kadir Siti Hamimah , Arshad Akmal Hisyam , Abdul Aziz Norhaslinda , Abdul Nasir Nurul Alimah , Ab Latip Normala
Published: March 2022

Vitamin E is known as an essential vitamin, and many studies had demonstrated the importance of vitamin E throughout the reproductive process, such as miscarriage, premature birth, preeclampsia, and intrauterine growth restriction, which could be caused by a lack of vitamin E during pregnancy. Its potent antioxidant properties can counteract the oxidative stress induced by oxygen free radicals and imbalance of oxidative-antioxidant levels, hence it may play a role in maintaining the normal function of the female reproductive system. Despite the fact that vitamin E is acknowledged as the substance needed for reproduction, its beneficial effects on female fertility, gynaecological health, and diseases are still poorly understood and lacking. Therefore, the goal of this paper is to provide a summary of the known roles of vitamin E supplementation in women for gynaecological health and reproductive-related diseases, as well as its future perspective.
Laurus nobilis L. Essential Oil-Loaded PLGA as a Nanoformulation Candidate for Cancer Treatment
Authors: Ercin Esin , Kecel-Gunduz Serda , Gok Bahar , Aydin Tugba , Budama-Kilinc Yasemin , Kartal Murat
Published: March 2022

The aim of this study was to obtain essential oil (LNEO) from the Laurus nobilis L. plant, and to prepare LNEO-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) as an approach in cancer treatment. The components of the obtained LNEO were analyzed using GC-MS. The LNEO-NPs were synthesized by the single-emulsion method. The LNEO-NPs were characterized using UV-Vis spectrometry, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and a DNA binding assay, which was performed via the UV-Vis titration method. According to the results, the LNEO-NPs had a 211.4 ± 4.031 nm average particle size, 0.068 ± 0.016 PdI, and −7.87 ± 1.15 mV zeta potential. The encapsulation efficiency and loading capacity were calculated as 59.25% and 25.65%, respectively, and the in vitro drug release study showed an LNEO release of 93.97 ± 3.78% over the 72 h period. Moreover, the LNEO was intercalatively bound to CT-DNA. In addition, the mechanism of action of LNEO on a dual PI3K/mTOR inhibitor was predicted, and its antiproliferative activity and mechanism were determined using molecular docking analysis. It was concluded that LNEO-loaded PLGA NPs may be used for cancer treatment as a novel phytotherapeutic agent-based controlled-release system.
An Insight into Antihyperlipidemic Effects of Polysaccharides from Natural Resources
Authors: Jing Yong-Shuai , Ma Yun-Feng , Pan Fei-Bing , Li Ming-Song , Zheng Yu-Guang , Wu Lan-Fang , Zhang Dan-Shen
Published: March 2022

Hyperlipidemia is a chronic metabolic disease caused by the abnormal metabolism of lipoproteins in the human body. Its main hazard is to accelerate systemic atherosclerosis, which causes cerebrovascular diseases such as coronary heart disease and thrombosis. At the same time, although the current hypolipidemic drugs have a certain therapeutic effect, they have side effects such as liver damage and digestive tract discomfort. Many kinds of polysaccharides from natural resources possess therapeutic effects on hyperlipidemia but still lack a comprehensive understanding. In this paper, the research progress of natural polysaccharides on reducing blood lipids in recent years is reviewed. The pharmacological mechanisms and targets of natural polysaccharides are mainly introduced. The relationship between structure and hypolipidemic activity is also discussed in detail. This review will help to understand the value of polysaccharides in lowering blood lipids and provide guidance for the development and clinical application of new hypolipidemic drugs.
Multi-Omics Characterization of Type 2 Diabetes Mellitus-Induced Cognitive Impairment in the db/db Mouse Model
Authors: Song Xiaoxuan , Zhu Zeyu , Qian Xiaohang , Liu Xiaoli , Chen Shengdi , Tang Huidong
Published: March 2022

Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder frequently accompanied by cognitive impairment. Contributing factors such as modern lifestyle, genetic predisposition, and gene environmental interactions have been postulated, but the pathogenesis remains unclear. In this study, we attempt to investigate the potential mechanisms and interventions underlying T2DM-induced cognitive deficits from the brain–gut axis perspective. A combined analysis of the brain transcriptome, plasma metabolome, and gut microbiota in db/db mice with cognitive decline was conducted. Transcriptome analysis identified 222 upregulated gene sets and 85 downregulated gene sets, mainly related to mitochondrial respiratory, glycolytic, and inflammation. In metabolomic analysis, a total of 75 significantly altered metabolites were identified, correlated with disturbances of glucose, lipid, bile acid, and steroid metabolism under disease state. Gut microbiota analysis suggested that the species abundance and diversity of db/db mice were significantly increased, with 23 significantly altered genus detected. Using the multi-omics integration, significant correlations among key genes (n = 33), metabolites (n = 41), and bacterial genera (n = 21) were identified. Our findings suggest that disturbed circulation and brain energy metabolism, especially mitochondrial-related disturbances, may contribute to cognitive impairment in db/db mice. This study provides novel insights into the functional interactions among the brain, circulating metabolites, and gut microbiota.
Microfluidic Microalgae System: A Review
Authors: Alias Anand Baby , Mishra Shubhanvit , Pendharkar Gaurav , Chen Chi-Shuo , Liu Cheng-Hsien , Liu Yi-Ju , Yao Da-Jeng
Published: March 2022

Microalgae that have recently captivated interest worldwide are a great source of renewable, sustainable and economical biofuels. The extensive potential application in the renewable energy, biopharmaceutical and nutraceutical industries have made them necessary resources for green energy. Microalgae can substitute liquid fossil fuels based on cost, renewability and environmental concern. Microfluidic-based systems outperform their competitors by executing many functions, such as sorting and analysing small volumes of samples (nanolitre to picolitre) with better sensitivities. In this review, we consider the developing uses of microfluidic technology on microalgal processes such as cell sorting, cultivation, harvesting and applications in biofuels and biosensing.
ICP-MS Multi-Elemental Analysis of the Human Meninges Collected from Sudden Death Victims in South-Eastern Poland
Authors: Baj Jacek , Teresiński Grzegorz , Kowalska Beata , Krajka Tomasz , Buszewicz Grzegorz , Forma Alicja , Flieger Wojciech , Karakuła Kaja Hanna , Kędzierawski Paweł , Cywka Tomasz , Flieger Jolanta
Published: March 2022

Metals perform many important physiological functions in the human body. The distribution of elements in different tissues is not uniform. Moreover, some structures can be the site of an accumulation of essential or toxic metals, leading to multi-directional intracellular damage. In the nervous system, these disorders are especially dangerous. Metals dyshomeostasis has been linked to a variety of neurological disorders which end up leading to permanent injuries. The multi-elemental composition of the human brain is still the subject of numerous investigations and debates. In this study, for the first time, the meninges, i.e., the dura mater and the arachnoid, were examined for their elemental composition by means of inductively coupled plasma mass spectrometry (ICP-MS). Tissue samples were collected post mortem from those who died suddenly as a result of suicide (n = 20) or as a result of injuries after an accident (n = 20). The interactions between 51 elements in both groups showed mainly weak positive correlations, which dominated the arachnoid mater compared to the dura mater. The study showed differences in the distribution of some elements within the meninges in the studied groups. The significant differences concerned mainly metals from the lanthanide family (Ln), macroelements (Na, K, Ca, Mg), a few micronutrients (Co), and toxic cadmium (Cd). The performed evaluation of the elemental distribution in the human meninges sheds new light on the trace metals metabolism in the central nervous system, although we do not yet fully understand the role of the human meninges.
Fullerenes against COVID-19: Repurposing C60 and C70 to Clog the Active Site of SARS-CoV-2 Protease
Authors: Marforio Tainah Dorina , Mattioli Edoardo Jun , Zerbetto Francesco , Calvaresi Matteo
Published: March 2022

The persistency of COVID-19 in the world and the continuous rise of its variants demand new treatments to complement vaccines. Computational chemistry can assist in the identification of moieties able to lead to new drugs to fight the disease. Fullerenes and carbon nanomaterials can interact with proteins and are considered promising antiviral agents. Here, we propose the possibility to repurpose fullerenes to clog the active site of the SARS-CoV-2 protease, Mpro. Through the use of docking, molecular dynamics, and energy decomposition techniques, it is shown that C60 has a substantial binding energy to the main protease of the SARS-CoV-2 virus, Mpro, higher than masitinib, a known inhibitor of the protein. Furthermore, we suggest the use of C70 as an innovative scaffold for the inhibition of SARS-CoV-2 Mpro. At odds with masitinib, both C60 and C70 interact more strongly with SARS-CoV-2 Mpro when different protonation states of the catalytic dyad are considered. The binding of fullerenes to Mpro is due to shape complementarity, i.e., vdW interactions, and is aspecific. As such, it is not sensitive to mutations that can eliminate or invert the charges of the amino acids composing the binding pocket. Fullerenic cages should therefore be more effective against the SARS-CoV-2 virus than the available inhibitors such as masinitib, where the electrostatic term plays a crucial role in the binding.
Enhancing the Anticancer Activity of Sorafenib through Its Combination with a Nitric Oxide Photodelivering β-Cyclodextrin Polymer
Authors: Laneri Francesca , Graziano Adriana C. E. , Seggio Mimimorena , Fraix Aurore , Malanga Milo , Béni Szabolcs , Longobardi Giuseppe , Conte Claudia , Quaglia Fabiana , Sortino Salvatore
Published: March 2022

In this contribution, we report a strategy to enhance the therapeutic action of the chemotherapeutic Sorafenib (SRB) through its combination with a multifunctional β-cyclodextrin-based polymer able to deliver nitric oxide (NO) and emit green fluorescence upon visible light excitation (PolyCDNO). The basically water-insoluble SRB is effectively encapsulated in the polymeric host (1 mg mL−1) up to a concentration of 18 μg mL−1. The resulting host-guest supramolecular complex is able to release SRB in sink conditions and to preserve very well the photophysical and photochemical properties of the free PolyCDNO, as demonstrated by the similar values of the NO release and fluorescence emission quantum efficiencies found. The complex PolyCDNO/SRB internalizes in HEP-G2 hepatocarcinoma, MCF-7 breast cancer and ACHN kidney adenocarcinoma cells, localizing in all cases mainly at the cytoplasmic level. Biological experiments have been performed at SRB concentrations below the IC50 and with light doses producing NO at nontoxic concentrations. The results demonstrate exceptional mortality levels for PolyCDNO/SRB upon visible light irradiation in all the different cell lines tested, indicating a clear synergistic action between the chemotherapeutic drug and the NO. These findings can open up exciting avenues to potentiate the anticancer action of SRB and, in principle, to reduce its side effects through its use at low dosages when in combination with the photo-regulated release of NO.
Production of Atosiban’s Key Intermediate Pentapeptide: Synthetic Approaches to the Development of a Peptide Synthesis with Less Racemization and Simplifier Purification Process
Authors: Ma Chunying , Zheng Yixuan , Liu Jinwei , Li Xiaobao , Feng Wenhua
Published: March 2022

The key intermediate NH2-Ile-Thr(Bzl)-Asn-Cys(Bzl)-Pro-COOH of Atosiban was prepared from N-Boc-S-Bzl-cysteine by the stepwise lengthening of the chain according to the repetitive N,O-bis(trimethylsilyl)acetamide/N-hydroxysuccinimide ester (BSA/NHS) strategy. This synthetic route required no chromatography purification and can be readily performed, yielding a highly pure pentapeptide compound.
Ultra-High Tg Thermoset Fibers Obtained by Electrospinning of Functional Polynorbornenes
Authors: Commarieu Basile , Compaoré Moubarak , de Boëver Raphaël , Imbeault Régis , Leprince Maxime , Martin Barbara , Perard Bruno , Qiu Weiguang , Claverie Jerome P.
Published: March 2022

Insertion polynorbornenes (PBNEs) are rigid-rod polymers that have very high glass transition temperatures (Tg). In this study, two functional PNBEs were electrospun in the presence of a variety of cross-linkers, resulting in fibers with Tgs greater than 300 °C. The fibers are long (several mm), rigid, and with diameters that can be tuned in the range 300 nm–10 μm. The electrospinning process can be used to encapsulate dyes or graphene dots. Due to the high cross-linking density of the fiber, dye leaching is prevented. In contrast with other rigid-rod polymers, electrospinning of PNBE is facile and can be performed at injection rates as high as 1 mL/min.
Surface Transformation of Spin-on-Carbon Film via Forming Carbon Iron Complex for Remarkably Enhanced Polishing Rate
Authors: Lee Jun-Myeong , Lee Jong-Chan , Kim Seong-In , Lee Seung-Jae , Bae Jae-Yung , Park Jin-Hyung , Park Jea-Gun
Published: March 2022

To scale down semiconductor devices to a size less than the design rule of 10 nm, lithography using a carbon polymer hard-mask was applied, e.g., spin-on-carbon (SOC) film. Spin coating of the SOC film produces a high surface topography induced by pattern density, requiring chemical–mechanical planarization (CMP) for removing such high surface topography. To achieve a relatively high polishing rate of the SOC film surface, the CMP principally requires a carbon–carbon (C-C) bond breakage on the SOC film surface. A new design of CMP slurry evidently accomplished C-C bond breakage via transformation from a hard surface with strong C-C covalent bonds into a soft surface with a metal carbon complex (i.e., C=Fe=C bonds) during CMP, resulting in a remarkable increase in the rate of the SOC film surface transformation with an increase in ferric catalyst concentration. However, this surface transformation on the SOC film surface resulted in a noticeable increase in the absorption degree (i.e., hydrophilicity) of the SOC film CMP slurry on the polished SOC film surface during CMP. The polishing rate of the SOC film surface decreased notably with increasing ferric catalyst concentration. Therefore, the maximum polishing rate of the SOC film surface (i.e., 272.3 nm/min) could be achieved with a specific ferric catalyst concentration (0.05 wt%), which was around seven times higher than the me-chanical-only CMP.
Low Adherence to the Mediterranean Diet Is Associated with Poor Socioeconomic Status and Younger Age: A Cross-Sectional Analysis of the EpiDoC Cohort
Authors: Mendonça Nuno , Gregório Maria João , Salvador Clara , Henriques Ana Rita , Canhão Helena , Rodrigues Ana M.
Published: March 2022

The Mediterranean diet (MD) is recognized as one of the healthiest dietary patterns as it has been consistently associated with several beneficial health outcomes. Adherence to the MD pattern has been decreasing in southern European countries for the last decades, especially among low socioeconomic groups. The aim of this study was to assess the adherence to the MD in Portugal, to evaluate regional differences, and explore associated factors (sociodemographic, economic, and lifestyles behaviors). This study used the third data collection wave of the Epidemiology of Chronic Diseases Cohort Study (EpiDoC 3). MD adherence was assessed using the Portuguese-validated MD adherence score (MEDAS) questionnaire. Non-adjusted and adjusted logistic regression models were used to assess the risk factors for low MD adherence and individual MEDAS items. In this cross-sectional evaluation of the EpiDoC 3 cohort study (n = 5647), 28.8% of the Portuguese population had low adherence to a MD. Azores and Madeira had lower adherence to the MD than the rest of the country. Younger individuals in lower income categories (e.g., ORfinding it very difficult = 1.48; 95% CI 1.16–1.91) and with a lower educational level (e.g., OR0–4 years = 2.63; 95% CI 2.09–3.32) had higher odds of having a lower adherence to the MD. Portuguese adults have a high prevalence of low adherence to the MD, especially among those who are younger and have lower socioeconomic status. Public health policies to promote adherence to the MD should pay special attention to these groups.