bluuebunny/crossref_metadata_2025 huggingface dataset:
Discovery and Biology of Spathius verustus Chao (Hymenoptera: Braconidae), a Potential Biological Agent on Two Monochamus Vectors of the Pinewood Nematode
Authors: Kim Moo-Sung , Jung Jong-Kook , Hong Ki-Jeong , Kim Chang-Jun , Lee Bong-Woo , Kim Il-Kwon
Published: June 2022

Pine wilt disease in Korea can be managed by reducing vector density through chemical application. To reduce the side effects from pesticides, effective natural enemies must be identified and used to reduce the vectors’ natural density. Sentinel logs were used to investigate a parasitoid wasp parasitic to Monochamus alternatus and Monochamus saltuarius, the vectors of Bursaphelenchus xylophilus, which causes this disease. During 2016–2017, the parasitoid wasps distributed in the Pinus densiflora and Pinus koraiensis forests were surveyed using sentinel logs at six different areas. An ectoparasitoid wasp Spathius verustus Chao of M. alternatus and M. saltuarius was identified. We showed for the first time that S. verustus was parasitic to M. alternatus in South Korea and to M. saltuarius worldwide. The parasitism rates were affected by region and session but not by the sentinel log height in the tree and the distances between the trap and forest edge locations. Studies also showed that S. verustus appeared to prefer M. alternatus to M. saltuarius as a host. This study unveiled the ecological details of S. verustus. Further research on various environmental factors such as regional differences, host density differences, and the degree of damage from the pine wilt disease is required to understand the effects of environmental or ecological factors on parasitism rates.
Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (Procambarus clarkii) from Lakes and Rice Fields by SSR Markers
Authors: Guo Xin-Fen , Liu Min , Zhou Yu-Lin , Wei Wen-Yu , Li Zhi , Zhou Li , Wang Zhong-Wei , Gui Jian-Fang
Published: June 2022

The red swamp crayfish (Procambarus clarkii) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally developed to assess the genetic diversities of seven wild populations from natural lakes and nine cultured populations from rice fields. Genetic diversity was slightly higher in the cultured populations than in the wild populations. The degree of genetic differentiation between cultured populations is slight, while a moderate to a large degree of genetic differentiation between wild populations and most of the variations occurred within individuals (79%). The analysis of cluster, principal coordinate analysis and STRUCTURE were similar, and they showed that isolation-by-distance pattern was not significant. The microsatellite markers developed in this study can not only be used for genetic monitoring of population but also provide important information for the management of breeding and cultured population in red swamp crayfish.
Characterization of a Commercial Whey Protein Hydrolysate and Its Use as a Binding Agent in the Whey Protein Isolate Agglomeration Process
Authors: Zaitoun Baheeja J. , Palmer Niels , Amamcharla Jayendra K.
Published: June 2022

The first objective of this study was to characterize the chemical properties of three lots of whey protein hydrolysate (WPH) obtained from a commercial manufacturer. The degree of hydrolysis (DH) of WPH was between 13.82 and 15.35%, and was not significantly (p > 0.05) different between the batches. From MALDI-TOF, 10 to 13 different peptides were observed in the range of 2.5–5 kDa and 5–8 kDa, respectively. The second objective of the study was to evaluate the effectiveness of WPH as a binder in whey protein isolate (WPI) wet agglomeration. For this purpose, a 3 × 3 × 2 factorial design was conducted with pre-wet mass (60, 100, and 140 g), WPH concentration (15, 20, and 25%), and flow rate (4.0 and 5.6 mL·min−1) as independent variables. WPI agglomeration was carried out in a top-spray fluid bed granulator (Midi-Glatt, Binzen, Germany). Agglomerated WPI samples were stored at 25 °C and analyzed for moisture content (MC), water activity, relative dissolution index (RDI), and emulsifying capacity. Pre-wet mass, flow rate, and the WPH concentration had a significant (p < 0.05) effect on the MC. Moreover, all interactions among the main effects had also a significant (p < 0.05) effect on MC. High MC and water activity were observed for the treatments with a higher pre-wet volume and higher flow rate, which also resulted in clumping of the powders. The treatment with the 60 g pre-wet mass, 20% WPH concentration, and 5.6 mL·min−1 flow rate combination had the highest RDI among all the samples. In conclusion, WPH can be used as a potential alternative to soy lecithin in WPI wet agglomeration.
Effect of Drought Stress on Degradation and Remodeling of Membrane Lipids in Nostoc flagelliforme
Authors: Wang Meng , Zhu Qiang , Li Xiaoxu , Hu Jinhong , Song Fan , Liang Wangli , Ma Xiaorong , Wang Lingxia , Liang Wenyu
Published: June 2022

Nostoc flagelliforme is a kind of terrestrial edible cyanobacteria with important ecological and economic value which has developed special mechanisms to adapt to drought conditions. However, the specific mechanism of lipidome changes in drought tolerance of N. flagelliforme has not been well understood. In this study, the ultra-high-performance liquid chromatography and mass spectrometry were employed to analyze the lipidome changes of N. flagelliforme under dehydration. A total of 853 lipid molecules were identified, of which 171 were significantly different from that of the control group. The digalactosyldiacylglycerol/monogalactosyldiacylglycerol (DGDG/MGDG) ratio was increased. The amount of wax ester (WE) was sharply decreased during drought stress, while Co (Q10) was accumulated. The levels of odd chain fatty acids (OCFAs) were increased under dehydration, positively responding to drought stress according to the energy metabolism state. In conclusion, the lipidomic data corroborated that oxidation, degradation, and biosynthesis of membrane lipids took place during lipid metabolism, which can respond to drought stress through the transformation of energy and substances. Besides, we constructed a lipid metabolic model demonstrating the regulatory mechanism of drought stress in N. flagelliforme. The present study provides insight into the defense strategies of cyanobacteria in lipid metabolic pathways.
Frozen-Phase High-Pressure Destruction Kinetics of Escherichia coli as Influenced by Application Mode, Substrate, and Enrichment Medium
Authors: Wang Chunfang , Liu Hongru , Yu Yong , Qiao Yongjin
Published: June 2022

The synergistic effect of frozen-phase high pressure (HP) on the inactivation of E. coli ATCC 25922 cultures in suspension medium, Chinese bayberry juice (pH 3.0), and carrot juice (pH 6.5) was evaluated. The survivor count of E. coli remained at 3.36 log CFU/mL on a nonselective brain heart infusion (BHIA) medium, while no survivor was detected on a selective violet red bile agar (VRBA) medium after a 5 min hold pressure at 250 MPa in a frozen culture suspension. BHIA was suitable for safe testing of the injured E coli cells after HP treatment in frozen state. Frozen Chinese bayberry juice showed higher sensitivity to HP treatment for its matrix property with high sterilizing efficiency at 170 MPa. Two pulses exhibited a significant inactivation effect in frozen samples compared with one pulse, especially for the Chinese bayberry juice with different pressure levels. The destruction kinetics of HP pulse mode followed the first-order rate kinetics with a Zp value of 267 MPa in frozen carrot juice. Our results evaluated the influenced factors of frozen HP destruction effects, including the medium, substrate, and application mode. The frozen HP destruction kinetics of pulses afford us better understanding of the technology application in the food industry.
Structural Characterization and Hypoglycemic Activity of a Novel Pumpkin Peel Polysaccharide-Chromium(III) Complex
Authors: Zhang Wen , Li Lingyu , Ma Yue , Chen Xiaole , Lan Tao , Chen Long , Zheng Zhenjia
Published: June 2022

The aim of our study was to synthesize a pumpkin peel polysaccharide (PPP)-Cr(III) complex and investigate its hypoglycemic activity. Firstly, a novel PPP-Cr(III) complex with a Cr content of 23.77 mg/g was synthesized and characterized. Physicochemical characterization indicated that PPP-Cr(III) had some changes in chemical composition, monosaccharide composition, and morphological structure compared with PPP. The molecular weights of PPP-Cr(III) and PPP were 1.398 × 106 g/mol and 3.386 × 106 g/mol, respectively, showing a lower molecular weight after the introduction of Cr(III). Fourier transform infrared spectroscopy showed that a new characteristic absorption peak of Cr-O appeared at 534 cm−1 in PPP-Cr(III), indicating that Cr(III) was successfully complexed with PPP. Secondly, the hypoglycemic activity of PPP-Cr(III) based on α-glucosidase inhibitory and insulin resistance (IR)-HepG2 cells was evaluated. Compared with PPP, PPP-Cr(III) exhibited a more significantly α-glucosidase inhibitory activity. The IR-HepG2 cells confirmed an obvious increase in glucose consumption. Western blot analysis demonstrated that the treated IR-HepG2 cells were able to increase the protein levels of p-AMPK and p-GSK-3β, indicating that IR-HepG2 cells exerted hypoglycemic activity via the AMPK/GSK-3β signaling pathway. These results suggested that PPP-Cr(III) had good hypoglycemic activity, which could provide theoretical support for the development of novel hypoglycemic products.
Identification of the Core Promoter and Variants Regulating Chicken CCKAR Expression
Authors: Wang Zhepeng , Reid Angus M. A. , Wilson Peter W. , Dunn Ian C.
Published: June 2022

Decreased expression of chicken cholecystokinin A receptor (CCKAR) attenuates satiety, which contributes to increased food intake and growth for modern broilers. The study aims to define the core promoter of CCKAR, and to identify variants associated with expression activity. A 21 kb region around the CCKAR was re-sequenced to detect sequence variants. A series of 5′-deleted promoter plasmids were constructed to define the core promoter of CCKAR. The effects of sequence variants located in promoter (PSNP) and conserved (CSNP) regions on promoter activity were analyzed by comparing luciferase activity between haplotypes. A total of 182 variants were found in the 21 kb region. There were no large structural variants around CCKAR. pNL−328/+183, the one with the shortest insertion, showed the highest activity among the six promoter constructs, implying that the key cis elements regulating CCKAR expression are mainly distributed 328 bp upstream. We detected significant activity differences between high- and low-growth associated haplotypes in four of the six promoter constructs. The high-growth haplotypes of constructs pNL−1646/+183, pNL−799/+183 and pNL−528/+183 showed lower activities than the low-growth haplotypes, which is consistent with decreased expression of CCKAR in high-growth chickens. Lower expression of the high-growth allele was also detected for the CSNP5-containing construct. The data suggest that the core promoter of CCKAR is located the 328 bp region upstream from the transcription start site. Lower expression activities shown by the high-growth haplotypes in the reporter assay suggest that CSNP5 and variants located between 328 bp and 1646 bp upstream form a promising molecular basis for decreased expression of CCKAR and increased growth in chickens.
Characterization of Immune-Based Molecular Subtypes and Prognostic Model in Prostate Adenocarcinoma
Authors: Guo Li , Kang Yihao , Xia Daoliang , Ren Yujie , Yang Xueni , Xiang Yangyang , Tang Lihua , Ren Dekang , Yu Jiafeng , Wang Jun , Liang Tingming
Published: June 2022

Prostate adenocarcinoma (PRAD), also named prostate cancer, the most common visceral malignancy, is diagnosed in male individuals. Herein, in order to obtain immune-based subtypes, we performed an integrative analysis to characterize molecular subtypes based on immune-related genes, and further discuss the potential features and differences between identified subtypes. Simultaneously, we also construct an immune-based risk model to assess cancer prognosis. Our findings showed that the two subtypes, C1 and C2, could be characterized, and the two subtypes showed different characteristics that could clearly describe the heterogeneity of immune microenvironments. The C2 subtype presented a better survival rate than that in the C1 subtype. Further, we constructed an immune-based prognostic model based on four screened abnormally expressed genes, and they were selected as predictors of the robust prognostic model (AUC = 0.968). Our studies provide reference for characterization of molecular subtypes and immunotherapeutic agents against prostate cancer, and the developed robust and useful immune-based prognostic model can contribute to cancer prognosis and provide reference for the individualized treatment plan and health resource utilization. These findings further promote the development and application of precision medicine in prostate cancer.
Toxoplasma gondii Seropositivity Interacts with Catechol-O-methyltransferase Val105/158Met Variation Increasing the Risk of Schizophrenia
Authors: Rovira Paula , Gutiérrez Blanca , Sorlózano-Puerto Antonio , Gutiérrez-Fernández José , Molina Esther , Rivera Margarita , Martínez-Leal Rafael , Ibanez-Casas Inmaculada , Martín-Laguna María Victoria , Rosa Araceli , Torres-González Francisco , Cervilla Jorge A.
Published: June 2022

Schizophrenia is a heterogeneous and severe psychotic disorder. Epidemiological findings have suggested that the exposure to infectious agents such as Toxoplasma gondii (T. gondii) is associated with an increased risk for schizophrenia. On the other hand, there is evidence involving the catechol-O-methyltransferase (COMT) Val105/158Met polymorphism in the aetiology of schizophrenia since it alters the dopamine metabolism. A case–control study of 141 patients and 142 controls was conducted to analyse the polymorphism, the prevalence of anti-T. gondii IgG, and their interaction on the risk for schizophrenia. IgG were detected by ELISA, and genotyping was performed with TaqMan Real-Time PCR. Although no association was found between any COMT genotype and schizophrenia, we found a significant association between T. gondii seropositivity and the disorder (χ2 = 11.71; p-value < 0.001). Furthermore, the risk for schizophrenia conferred by T. gondii was modified by the COMT genotype, with those who had been exposed to the infection showing a different risk compared to that of nonexposed ones depending on the COMT genotype (χ2 for the interaction = 7.28, p-value = 0.007). This study provides evidence that the COMT genotype modifies the risk for schizophrenia conferred by T. gondii infection, with it being higher in those individuals with the Met/Met phenotype, intermediate in heterozygous, and lower in those with the Val/Val phenotype.
Impact of an Educational Program on Improving Nurses’ Management of Fever: An Experimental Study
Authors: Hsiao Bi-Hung , Tzeng Ya-Ling , Lee Kwo-Chen , Lu Shu-Hua , Lin Yun-Ping
Published: June 2022

Background: Despite a public information campaign “To Break the Myth of Fever”, nurses continued to overtreat fever. This study hypothesized that the campaign lacked the detailed rationale essential to alter nurses’ attitudes and behaviors. Aim: To evaluate the effect of the educational program on nurses’ knowledge, attitudes, and behaviors related to fever management. Design: A randomized experimental design using a time series analysis. Methods: A random sample of 58 medical/surgical nurses was evenly divided into an intervention and a control group. The intervention group received an educational program on fever and fever management. Both groups completed a pretest and four posttests using investigator-developed instruments: a questionnaire on knowledge and attitudes about fever management and a fever treatment checklist to audit charts. Results: The intervention group had markedly higher knowledge scores and reduced use of ice pillows at all four posttests, as well as lower use of antipyretics overall, except for the first posttest, despite no sustained change in attitude. Conclusions: An educational program for fever management can effectively improve clinical nurses’ knowledge and attitudes about fever management.
Assessment of Diagnostic Imaging Sector in Public Hospitals in Northern Jordan
Authors: Oglat Ammar A.
Published: June 2022

The most effective diagnostic methods in the medical field are diagnostic imaging techniques such as radiography, computed tomography (CT), magnetic resonance imaging (MRI), and nuclear medicine, which are used to visualize internal body to diagnose it, determine potential treatment, and evaluate and forecast care results. Therefore, the purpose of this research is to assess the diagnostic imaging sector, at three major public hospitals in the northern part of Jordan, according to regional and global requirements. The assessment approach was based on knowledge of the accessibility of diagnostic imaging equipment and its quality assurance and performance, the quantity and efficiency of radiological technologists, and the design of radiology units and medical imaging chambers in many aspects based on the use of two tools, a questionnaire and checklists, to accomplish a comprehensive evaluation. The response rate of radiological technologists was 66%. The assessment reveals a noticeable increase in the number of radiological technologists in general with high academic qualification level. Additionally, the number of diagnostic imaging equipment in Jordan revealed a large deficiency in the population–device balance, and through checklists that evaluated both CT and MRI units, it was revealed that the rate of following global requirements and occupational health and safety (OHS) standards was high. The basic supplies available in both the CT and MRI units alike were high, which indicates the high quality of healthcare provided in Jordan.
The Efficacy of Machine-Learning-Supported Smart System for Heart Disease Prediction
Authors: Absar Nurul , Das Emon Kumar , Shoma Shamsun Nahar , Khandaker Mayeen Uddin , Miraz Mahadi Hasan , Faruque M. R. I. , Tamam Nissren , Sulieman Abdelmoneim , Pathan Refat Khan
Published: June 2022

The disease may be an explicit status that negatively affects human health. Cardiopathy is one of the common deadly diseases that is attributed to unhealthy human habits compared to alternative diseases. With the help of machine learning (ML) algorithms, heart disease can be noticed in a short time as well as at a low cost. This study adopted four machine learning models, such as random forest (RF), decision tree (DT), AdaBoost (AB), and K-nearest neighbor (KNN), to detect heart disease. A generalized algorithm was constructed to analyze the strength of the relevant factors that contribute to heart disease prediction. The models were evaluated using the datasets Cleveland, Hungary, Switzerland, and Long Beach (CHSLB), and all were collected from Kaggle. Based on the CHSLB dataset, RF, DT, AB, and KNN models predicted an accuracy of 99.03%, 96.10%, 100%, and 100%, respectively. In the case of a single (Cleveland) dataset, only two models, namely RF and KNN, show good accuracy of 93.437% and 97.83%, respectively. Finally, the study used Streamlit, an internet-based cloud hosting platform, to develop a computer-aided smart system for disease prediction. It is expected that the proposed tool together with the ML algorithm will play a key role in diagnosing heart diseases in a very convenient manner. Above all, the study has made a substantial contribution to the computation of strength scores with significant predictors in the prognosis of heart disease.
Religiosity, Emotions and Health: The Role of Trust/Mistrust in God in People Affected by Cancer
Authors: Almaraz David , Saiz Jesús , Moreno Martín Florentino , Sánchez-Iglesias Iván , Molina Antonio J. , Goldsby Tamara L. , Rosmarin David H.
Published: June 2022

Trust in God implies the conviction that God looks after a person’s own interests. The first evidence of a relationship between this construct and people’s psychological and emotional health dates back several centuries. However, the literature on this is limited, especially for people with physical health conditions, such as cancer. Therefore, the purpose of this study is to test the relationships between trust/mistrust in God, social support and emotions in people affected by cancer. The sample consisted of 177 women and men in Spain diagnosed with cancer. The instruments used were the Trust/Mistrust in God Scale, the Positive and Negative Affect Schedule and the Multidimensional Scale of Perceived Social Support. Correlation analysis and hierarchical regression analysis were performed to compare several explanatory models for the dependent variables: positive and negative emotions. The results show significant relationships between all variables. It was observed that, when trust/mistrust in God is included in the model, only mistrust in God predicts both types of emotions. In addition, both social support and some sociodemographic variables help to predict the dependent variables. This study shows that valuing the religiosity and spirituality of oncology patients in healthcare settings can have a significant positive impact on the health of these individuals. Moreover, it represents an important approach to the study of trust/mistrust in God in the context of a traditionally Catholic country.
An Electrostatic Pest Exclusion Strategy for Greenhouse Tomato Cultivation
Authors: Kakutani Koji , Matsuda Yoshinori , Nonomura Teruo , Toyoda Hideyoshi
Published: June 2022

Electrostatic devices generating an electric field (EF) are promising tools for greenhouse tomato cultivation. In these devices, an EF is generated in the space surrounding an insulated conductor (IC) that is charged by a voltage generator. Thus, a physical force is exerted on any insect that enters the EF, as a negatively charged IC (NC-IC) pushes a negative charge (free electrons) out of the insect body. The insect is polarized positively to be attracted to the NC-IC, and a grounded metal net (G-MN) repels the insect. This dual function of the apparatus (insect capture and repulsion) is the core of the electrostatic pest-exclusion strategy. In this study, we applied various innovative EF-based devices to evaluate their efficacy in greenhouse tomato cultivation. Our objective was to determine the optimal apparatus for simple, inexpensive construction by greenhouse workers. The results of this study will contribute to the development of sustainable pest-management protocols in greenhouse horticulture.
The Influence of Harvest Moment and Cultivar on Variability of Some Chemical Constituents and Antiradical Activity of Dehydrated Chokeberry Pomace
Authors: Mazilu Ivona Enescu , Vîjan Loredana Elena , Cosmulescu Sina
Published: June 2022

This paper’s aim was to study how the antioxidant activity and the level of certain phenolic complexes and carotenoids vary in the pomace obtained from the fruits of two cultivars of chokeberry at different times of harvest after reaching the stage of maturity. The influence of the cultivar, harvest moment, and the combined effect of these two factors on the antioxidant activity and the dehydrated pomace content in components with antioxidant potentials, such as total phenolics, total tannins, total flavonoids, lycopene, and β-carotene was analyzed. The methanolic extract from the pomace obtained from the ‘Melrom’ cultivar had the highest efficiency (92.14 ± 5.02%). The antiradical activity of the pomace was maximal (93.27 ± 4.32%) after the middle of the harvest season (3 September). The pomace obtained from the ‘Nero’ cultivar displayed superior levels of phenolic content (13,030.16 ± 1414.46 mg/100 g), flavonoids (4627.83 ± 509.63 mg CE/100 g), tannins (7458.56 ± 529.43 mg/100 g), and lycopene (1.171 ± 0.388 mg/100 g). The ‘Melrom’ cultivar presented superior content of β-carotene (0.313 ± 0.07 mg/100 g). On average, a positive significant correlation between radical scavenging activity with total phenolic content and β-carotene was observed. The combined cultivar × harvest moment effect was reflected in the variations in the total tannins content and the total flavonoid content, but also in the antiradical activity of the methanolic extracts. Dehydrated pomace from chokeberry fruit can be an important source of antioxidant biological compounds and can be used to make innovative foods.
Surface Moisture Induces Microcracks and Increases Water Vapor Permeance of Fruit Skins of Mango cv. Apple
Authors: Athoo Thomas O. , Winkler Andreas , Owino Willis O. , Knoche Moritz
Published: June 2022

Exposure to surface moisture triggers cuticular microcracking of the fruit skin. In mango fruit cv. apple, microcracking compromises postharvest performance by increasing moisture loss and infections with pathogens. This study reports the effects of exposing the fruit’s skin to surface moisture on the incidence of microcracking and on water vapor permeance. Microcracking was quantified microscopically following infiltration with a fluorescent tracer. Water mass loss was determined gravimetrically. Moisture exposure increased cuticular microcracking and permeance. During moisture exposure, permeance increased over the first 4 d, remained constant up to approximately 8 d, then decreased for longer exposure times. Fruit development followed a sigmoid growth pattern. The growth rate peaked approximately 103 days after full bloom. This coincided with the peak in moisture-induced microcracking. There were no increases in water vapor permeance or in microcracking in control fruit that remained dry. When experimental moisture exposure was terminated, microcracking and water vapor permeance decreased. This suggests a repair process restoring the barrier properties of the fruit skin. Histological analyses reveal a periderm forms in the hypodermis beneath a microcrack. Our study demonstrates that surface moisture induces microcracking in mango cv. apple that increases the skin’s water vapor permeance and induces russeting.
Effect of Foaming Conditions on Foam Properties and Drying Behavior of Powder from Magenta (Peristropheroxburghiana) Leaves Extracts
Authors: Thuy Nguyen Minh , Tien Vo Quoc , Van Tai Ngo , Minh Vo Quang
Published: June 2022

In manufacturing food powders, foam-mat drying provides a cost-effective alternative to traditional drying methods. This study aimed to select the foaming conditions which support the subsequent drying of Magenta leaves extract. In the initial stage, concentrations of egg albumin (5 to 15%) as a foaming agent, xanthan gum as foam stabilizer (0.1 to 0.5%), and whipping time (2 to 8 min) were designed. Multiple regression analysis was applied to analyze the relationship between the dependent variables (Foam expansion volume and foam density) and three independent variables as an indicator of foaming capacity and foam stability. The multiple response optimization was applied to optimize the foam density and foam expansion. The optimum foam density (0.25 g/mL) and foam expansion volume (298.12%) were obtained at the optimum egg albumin and xanthan gum concentrations, and whipping time at 12.21%, 0.24%, and 5.8 min, respectively, indicating a stable foam structure. Experimental moisture loss data are fitted for five selected drying models. The mathematical models were compared according to three statistical parameters, such as coefficient of determination (R2), chi-square (χ2), and root mean square error (RMSE). Among the five mathematical models tested with experimental data, the Page model could be applied to describe the foam-mat drying process of magenta leaves extract. The highest value of R2 (99.54%), the lowest value of χ2 (0.0007), and RMSE (0.0253) were observed for a air drying temperature of 60 °C. The effect of temperature on diffusion is described by the Arrhenius equation with an activation energy of 100.21 kJ/mol. Effective moisture diffusion values ranged from 2.27 × 10−10 to 6.71 × 10−10 m2/s as the temperature increased. The effect of drying conditions on anthocyanin changes of magenta leaves powder was compared. The results showed that the highest quality of the sample was observed when the sample was dried at 60 °C.
Psychological Intervention in Women Victims of Childhood Sexual Abuse: An Open Study—Protocol of a Randomized Controlled Clinical Trial Comparing EMDR Psychotherapy and Trauma-Based Cognitive Therapy
Authors: Molero-Zafra Milagros , Mitjans-Lafont María Teresa , Hernández-Jiménez María Jesús , Pérez-Marín Marián
Published: June 2022

Introduction: Most victims of sexual abuse have symptoms that may lead to post-traumatic stress disorder. This study aims to offer evidence-based psychological treatment to women who have been sexually abused earlier in life and currently have sequelae from that trauma. With this treatment, each of the women in the study will hopefully improve their overall quality of life and, more specifically, it is expected that post-traumatic stress symptoms will decrease, as found in recent studies, as well as strengthening their security, confidence, and coping with the situations they have experienced. Methods and analysis: The effect of two therapeutic approaches focused on the improvement of trauma will be evaluated in a sample of 30–50 women victims of childhood sexual abuse, with a randomized clinical trial comparing EMDR psychotherapy and trauma-focused cognitive behavioral therapy. According to the literature reviewed, both approaches will considerably improve self-esteem when the appropriate number of sessions are conducted, significantly reducing general psychiatric symptoms and depression. Furthermore, the effects are sustained over time. It should be noted that this study will be carried out comparing both therapies, analyzing both the differential benefit of each and the cumulative effect of receiving both treatments and in which order. It is also intended to demonstrate that implementing the protocols presented in this study will help improve the quality of life of the women who benefit from them, and after this study, it will be possible to replicate this program in other people with the same problems. Each of the therapeutic benefits of each of them will be analyzed, and clinical and logistical guidance will be provided to implement both, including a session-by-session protocol.
The Relationship between the Hamstring-to-Quadriceps Ratio and Jumping and Sprinting Abilities of Young Male Soccer Players
Authors: Diker Gürkan , Struzik Artur , Ön Sadi , Zileli Raif
Published: June 2022

The correct torque ratio between the knee joint extensor and flexor muscle groups can effectively prevent injuries to the anterior cruciate ligament and hamstring strain. However, it is unclear whether a high torque ratio of the knee joint flexor muscles to the extensor muscles is beneficial for sport performance. Therefore, the aim of the study was to investigate the relationship between the hamstring-to-quadriceps (H/Q) ratio and sprint times (10- and 30-m) and jump heights (CMJ—countermovement jump and SJ—squat jump) in soccer players. The study examined 26 young elite soccer players (age: 18.1 ± 0.7 years; body height: 1.77 ± 0.05 m; body mass: 72.7 ± 5.7 kg). Knee joint flexor and extensor peak torques were assessed using the Cybex dynamometer (at 60°/s, 120°/s and 180°/s). Additionally, each participant performed the CMJ, SJ, and 30 m sprint. A significant relationship was obtained between the H/Q ratio (60°/s) and 30 m sprint time (r = 0.47). The positive direction of this relationship may indicate an important role of knee joint extensors in sprinting performance. Moreover, the H/Q ratio was not significantly associated with the CMJ, SJ or 10 m sprint performance. The H/Q ratio should be considered together with the peak torque values in terms of the assessment of sprinting and jumping performance.
The Impact of Charlson Comorbidity Index on the Functional Capacity of COVID-19 Survivors: A Prospective Cohort Study with One-Year Follow-Up
Authors: Núñez-Cortés Rodrigo , Malhue-Vidal Constanza , Gath Florencia , Valdivia-Lobos Gonzalo , Torres-Castro Rodrigo , Cruz-Montecinos Carlos , Martinez-Arnau Francisco M. , Pérez-Alenda Sofía , López-Bueno Rubén , Calatayud Joaquín
Published: June 2022

Objective: To determine the association between the Charlson comorbidity index (CCI) score after discharge with 6-min walk test (6MWT) 1 year after discharge in a cohort of COVID-19 survivors. Methods: In this prospective study, data were collected from a consecutive sample of patients hospitalized for COVID-19. The CCI score was calculated from the comorbidity data. The main outcome was the distance walked in the 6MWT at 1 year after discharge. Associations between CCI and meters covered in the 6MWT were assessed through crude and adjusted linear regressions. The model was adjusted for possible confounding factors (sex, days of hospitalization, and basal physical capacity through sit-to-stand test one month after discharge). Results: A total of 41 patients were included (mean age 58.8 ± 12.7 years, 20/21 men/women). A significant association was observed between CCI and 6MWT (meters): (i) crude model: β = −18.7, 95% CI = −34.7 to −2.6, p < 0.05; (ii) model adjusted for propensity score including sex, days of hospitalization, and sit-to-stand: β = −23.0, 95% CI = −39.1 to −6.8, p < 0.05. Conclusions: A higher CCI score after discharge indicates worse performance on the 6MWT at 1-year follow-up in COVID-19 survivors. The CCI score could also be used as a screening tool to make important clinical decisions.
CFD Numerical Simulation in Building Drainage Stacks as an Infection Pathway of COVID-19
Authors: Cheng Cheng-Li , Lin Yen-Yu
Published: June 2022

Being aware of global pandemics, this research focused on the potential infection routes in building drainage systems. Case studies have found that dysfunctional building drainage systems not only failed to block contaminants but also potentially became a route for the spreading of viruses. Several fluid simulations in pipelines were conducted in this research using COMSOL Multiphysics. In particular, virus transmission from one patient’s room to other uninfected residential units through pipelines was visualized. A 12-story building, which is commonly seen in the local area, was designed as a simulation model to visualize the transmission and analyze its hazards. Furthermore, five environmental factors were filtered out for discussion: distance, time span, pressure, initial concentration, and environment temperature. By manipulating these factors, the relationship between the factors and the behavior of the contaminant could be explored. In addition, a simulation with a different pipeline arrangement was included to observe the virus diffusion behavior under different scenarios. The visualized simulation concluded that the contaminant would spread through the drainage system and arrive at the neighboring four floors within an hour under the circumstances of a 12-story building with broken seals and constant pressure and contaminant supply on the seventh floor. Meanwhile, the whole building would be exposed to infection risks by the continuous virus spreading through a drainage system. Distance, time span, and pressure were considered critical factors that affected indoor contamination in the system. On the other hand, initial concentration and environmental temperature did not have significant roles. Visualizing the behavior of viruses provides a glimpse of what happens behind walls, paving the way for recognizing the importance of maintaining functional drainage systems for individuals’ health.
Decision Tree Analyses to Explore the Relevance of Multiple Sex/Gender Dimensions for the Exposure to Green Spaces: Results from the KORA INGER Study
Authors: Dandolo Lisa , Hartig Christina , Telkmann Klaus , Horstmann Sophie , Schwettmann Lars , Selsam Peter , Schneider Alexandra , Bolte Gabriele ,   on behalf of the INGER Study Group
Published: June 2022

Recently, attention has been drawn to the need to integrate sex/gender more comprehensively into environmental health research. Considering theoretical approaches, we define sex/gender as a multidimensional concept based on intersectionality. However, operationalizing sex/gender through multiple covariates requires the usage of statistical methods that are suitable for handling such complex data. We therefore applied two different decision tree approaches: classification and regression trees (CART) and conditional inference trees (CIT). We explored the relevance of multiple sex/gender covariates for the exposure to green spaces, measured both subjectively and objectively. Data from 3742 participants from the Cooperative Health Research in the Region of Augsburg (KORA) study were analyzed within the INGER (Integrating gender into environmental health research) project. We observed that the participants’ financial situation and discrimination experience was relevant for their access to high quality public green spaces, while the urban/rural context was most relevant for the general greenness in the residential environment. None of the covariates operationalizing the individual sex/gender self-concept were relevant for differences in exposure to green spaces. Results were largely consistent for both CART and CIT. Most importantly we showed that decision tree analyses are useful for exploring the relevance of multiple sex/gender dimensions and their interactions for environmental exposures. Further investigations in larger urban areas with less access to public green spaces and with a study population more heterogeneous with respect to age and social disparities may add more information about the relevance of multiple sex/gender dimensions for the exposure to green spaces.
The Impact of the COVID-19 Pandemic on Research and Volunteering Activities among Medical Students: A Cross-Sectional Study among Romanian and International Students from One Medical Faculty from Romania
Authors: Pop Andreea Iulia , Mirel Simona , Florea Mira , Lotrean Lucia Maria
Published: June 2022

Introduction: This study focuses on the influence of the COVID-19 pandemic on medical students in their last year of study at the Faculty of Medicine of the University of Medicine and Pharmacy in Cluj-Napoca, Romania. It analyzes the impact of social distance limits on the conduct of research activities required for the development of the graduation thesis, as well as assesses sixth-year students’ attitudes and behaviors toward volunteer activities during the COVID-19 pandemic. Methods: A cross-sectional survey was conducted in June–July 2021 among sixth-year students. It used an anonymous online questionnaire and was sent to all students from the Faculty of Medicine studying in the Romanian, English, or French sections (n = 606). The response rate was 38.28%, resulting in a final sample of 232 students from both Romanian and international sections. Results: The findings demonstrate that two-thirds of the students in the survey agreed that the COVID-19 pandemic had a substantial impact on their research. Some of the students had to choose another subject for their graduation thesis, while about a quarter of the students gave up collecting personal data, 25% of respondents stated that it was difficult to contact research participants, one-third of them linked the difficulty of doing activities at the hospital to the present outbreak, and almost 30% of the students had difficulty communicating with the coordinator. The disturbance of the research activity seems to be higher among international students in comparison with the Romanian students, while there are limited gender differences. One out of five medical students has been involved in volunteer activities during the pandemic. Most have opted to volunteer at hospitals (especially international students) and contribute to health education activities (especially women). Conclusions: This is the first study from Romania and one of the few from Europe that presents data regarding the influence of the COVID-19 pandemic on research and volunteering activities among medical students.
Are Coping Strategies, Emotional Abilities, and Resilience Predictors of Well-Being? Comparison of Linear and Non-Linear Methodologies
Authors: Lacomba-Trejo Laura , Mateu-Mollá Joaquín , Bellegarde-Nunes Monica D. , Delhom Iraida
Published: June 2022

Emotional intelligence (EI), problem-oriented coping, and resilience have been deeply studied as psychological predictors of wellbeing in stressful daily situations. The aim was to find out whether coping, EI, and resilience are predictors of well-being, using two statistical methodologies (hierarchical regression models and comparative qualitative models). With this objective in mind, we built an online evaluation protocol and administered it to 427 Spanish people, exploring these variables through a selection of validated tests. The extracted data were studied using linear predictive tests (hierarchical regression models), as well as fuzzy set qualitative comparative analysis. We found that EI variables had important associations with coping, positive affect, negative affect, and life satisfaction, and also acted as relevant predictors for all of them, together with resilience and problem-oriented coping. The fuzzy set qualitative comparative analysis showed a series of logical combinations of conditional causes and results of each potential configuration for these variables. The interaction between the presence of EI, resilience, and coping resulted in high levels of well-being. On the other hand, the presence of high emotional attention in interaction with low resilience and low coping abilities resulted in low well-being. These results increase knowledge about protective factors and allow for the creation of intervention programmes to enhance them.
Coping with the Experience of Bad Sleep and Fatigue Associated with the Nursing Clinical Practicum
Authors: Lai Mei-Hsin , Tzeng Chyn-Yuan , Chuang Yeu-Hui , Chang Pi-Chen , Chung Min-Huey
Published: June 2022

Nursing students experience anxiety during clinical practicum, which may interfere with their learning of clinical practice. This study explored the practicum anxiety symptom experience of clinical nursing students. The methodology was mixed research design consist of questionnaire and a qualitative research design following a grounded theory approach. Research data were gathered via theoretical sampling from 37 female college nursing students practicing in a Central Taiwan hospital and analyzed using the content analysis method. The mean age of the subjects was 20.7 ± 1.35 years old. The practicum-related anxiety symptom experience was made up of core dimensions associated with the nursing clinical practicum, such as bad sleep and fatigue, and covered six themes. Students first encountered anxiety-inducing situations in the clinical setting, and then they began coping through self-adaptation and teachers’ help. In cases of coping failure, students began to have a bad sleep in the night and then felt tired and fatigued all day. These themes became a repeating cycle during the clinical practicum. This conceptual model shows that students experienced bad sleep and fatigue as a result of anxiety symptoms during the clinical practicum. Bad sleep and fatigue are critical anxiety symptoms for nursing students in clinical practice. Nursing teachers should pay attention to bad sleep and fatigue in nursing students and help students to improve their mental and physical health.
Returning Home after Decontamination? Applying the Protective Action Decision Model to a Nuclear Accident Scenario
Authors: Rasmussen Joel , Wikström Petter B.
Published: June 2022

Studies of the aftermath of nuclear power plant accidents show that affected citizens assess higher risks and adopt more risk-avoidant behaviors than authorities expect. This results in differences between the planned recovery and actual outcomes. Based on this knowledge, this study examined the factors that affect citizens’ preference to continue living in a decontaminated area. Testing the key aspects of the protective action decision model (PADM), this study analyzed Swedish survey data (N = 2291) regarding such an accident scenario. Several aspects of the PADM, from the layperson’s view of threats and protective actions, to stakeholders and situational factors, were strongly supported. The most influential variables affecting settlement choices are perceptions of radiation risk, perceptions of decontamination effectiveness, government information, living with certain restrictions, and attachment to an area because of one’s work. A novel contribution of this study is that it ranked the significance of such effects on behavioral intentions in an emergency scenario. Regarding the policy recommendations, this study concluded that a recovery program must facilitate most aspects of people’s lives and provide trustworthy information on decontamination efficiency. As some people will avoid potential health risks and leave a decontaminated area, planning to implement one solution for everyone would likely not be optimal.
Protocol for a Systematic Review and Meta-Analysis of Observational Studies on the Association of Exposure to Toxic Environmental Pollutants and Left Ventricular Dysfunction
Authors: Linzalone Nunzia , Donzelli Gabriele , Morales Maria Aurora , Vozzi Federico
Published: June 2022

The association between environmental exposure to toxic substances and cardiovascular diseases (CVDs) in humans is widely recognized. However, the analysis of underlying pathophysiological mechanisms is essential to target meaningful endpoints of cardiotoxicity and allow a close-to-real life understanding of the role of chronic and acute exposure to multiple toxicants. The aim of this study is to outline the process for a systematic review of the literature that investigates the relationship between environmental pollution and left ventricular dysfunction. This systematic review and meta-analysis protocol will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA-P) statement. PubMed, Embase, and Web of Science databases will be searched without applying search filters. Two independent reviewers will screen all titles and abstracts and identify the articles to be included in the synthesis. The risk of bias (RoB) will be assessed using an instrument developed for non-randomized (i.e., observational) studies (NRS) of environmental exposures. The results of cohort, case-control, cross-sectional, time-series, and case-crossover studies will be extracted and presented in tables considering different population subgroups and length of exposure. This protocol will be expected to provide a sound basis for selecting toxic chemicals and pollutants to contribute with the epidemiological evidence to the in vitro testing protocol within the EU-funded ALTERNATIVE Project.