bluuebunny/crossref_metadata_2025 huggingface dataset:
Prediction of IGBT Gate Oxide Layer’s Performance Degradation Based on MultiScaleFormer Network
Authors: He Shilie , Yu Meiling , Chen Yiqiang , Zhou Zhenwei , Yu Lubin , Zhang Chao , Ni Yuanhua
Published: August 2024

Insulated gate bipolar transistors (IGBTs) are widely used in power electronic devices, and their health prediction problems have attracted much attention in the field of power electronic equipment health management. The performance degradation of IGBT gate oxide is one of the most important failure modes. In order to analyze this failure mechanism and the ease of implementation of a monitoring circuit, the gate leakage current of IGBTs was selected as the fault precursor parameter for the degradation of their gate oxide performance, and feature selection and fusion were carried out by using time domain characteristic analysis, grayscale correlation, Mahalanobis distance, Kalman filter, and other methods. Thus, a health indicator was obtained to characterize the degradation of IGBT performance, which was used to indicate the degree of aging of the IGBT gate oxide layer. In this paper, we propose an improved degradation prediction model called MultiScaleFormer, inspired by advanced design ideas of the iTransformer network architecture, combined with the health parameters of IGBTs to construct a degradation prediction model for the IGBT gate oxide layer. MultiScaleFormer showed the highest fitting accuracy compared with the Long Short-Term Memory (LSTM), Convolutional Neural Network (CNN), Support Vector Regression (SVR), Gaussian Process Regression (GPR), CNN-LSTM, and Transformer models in our experiment. The mean absolute error (MAE) of the MultiScaleFormer prediction was as low as 0.0087. Extraction of the health indicator and the construction and verification of the degradation prediction model were carried out on the dataset released by the NASA-Ames Laboratory. These results demonstrate the feasibility of the gate leakage current as a fault precursor parameter for IGBT gate oxide failure, and the feasibility and accuracy of the MultiScaleFormer prediction model for IGBT performance degradation.
Reliability Simulation Analysis of TSV Structure in Silicon Interposer under Temperature Cycling
Authors: Tian Wenchao , Dang Haojie , Li Dexin , Cong Yunhao , Chen Yuanming
Published: August 2024

As semiconductor integration scales expand and chip sizes shrink, Through Silicon Via (TSV) technology advances towards smaller diameters and higher aspect ratios, posing significant challenges in thermo-mechanical reliability, particularly within interposer substrates where mismatched coefficients of thermal expansion exacerbate issues. This study conducts a thermo-mechanical analysis of TSV structures within multi-layered complex interposers, and analyzes the thermal stress behavior and reliability under variable temperature conditions (−55 °C to 85 °C), taking into account the typical electroplating defects within the copper pillars in TSVs. Initially, an overall model is established to determine the critical TSV locations. Sub-model analysis is then employed to investigate the stress and deformation of the most critical TSV, enabling the calculation of the temperature cycle life accordingly. Results indicate that the most critical TSV resides centrally within the model, exhibiting the highest equivalent stress. During the temperature cycling process, the maximum deformation experiences approximately periodic variations, while the maximum equivalent stress undergoes continuous accumulation and gradually diminishes. Its peak occurs at the contact interface corner between the TSV and Redistribution Layer (RDL). The estimated life of the critical point is 3.1708 × 105 cycles. Furthermore, it is observed that electroplating defect b alleviates thermal stress within TSVs during temperature cycling. This study provides insights into TSV thermal behavior and reliability, which are crucial for optimizing the design and manufacturing processes of advanced semiconductor packaging.
Design and Fabrication of High-Performance Piezoelectric Micromachined Ultrasonic Transducers Based on Aluminum Nitride Thin Films
Authors: Zhang Le , Yan Kunxian , Ye Lei , Luo Xiangyu , He Jian , Chou Xiujian
Published: August 2024

Ultrasound is widely applied in diverse domains, such as medical imaging, non-destructive evaluation, and acoustic communication. Piezoelectric micromachined ultrasonic transducers (PMUTs) capable of generating and receiving ultrasonic signals at the micrometer level have become a prominent technology in the field of ultrasound. It is important to enrich the models of the PMUTs to meet the varied applications. In this study, a series of PMUT devices featured with various top electrode configurations, square, circular, and doughnut, were designed to assess the influence of shape on the emission efficacy. It was demonstrated that the PMUTs with a circular top electrode were outperformed, which was calculated from the external acoustic pressure produced by the PMUTs operating in the fundamental resonant mode at a specified distance. Furthermore, the superior performance of PMUT arrays were exhibited through computational simulations for the circular top electrode geometries. Conventional microelectromechanical systems (MEMS) techniques were used to fabricate an array of PMUTs based on aluminum nitride (AlN) films. These findings make great contributions for enhancing the signal transmission sensitivity and bandwidth of PMUTs, which have significant potential in non-destructive testing and medical imaging applications.
Key Technologies for Surface-Borehole Transient Electromagnetic Systems and Applications
Authors: Guo Qingming , Mao Yurong , Yan Liangjun , Chen Wenhui , Yang Jupeng , Xie Xingbing , Zhou Lei , Li Haojin
Published: August 2024

The nonferrous metal mines in China are rapidly depleting due to years of mining, and it has become difficult to identify new mineral resources in the periphery of the old mining area. In order to deal with this situation, advanced technologies and equipment must be deployed. The borehole transient electromagnetic method (TEM) has become a key technology due to its deep investigative capabilities within conductive geological structures. In the present study, in order to meet the exploration needs at depths of less than 3000 m, surface-borehole TEM exploration was used to analyze the characteristics of electromagnetic signals generated by a long wire source and a large loop source, providing essential data for the development of key technologies, such as sensor parameter design and signal gain optimization of the TEM system in the borehole. This study discussed in detail two key technical problems as follows: firstly, the efficient synchronization mechanism between the ground transmitter system and the borehole electromagnetic signal acquisition system ensured the accuracy and timeliness of data acquisition; and secondly, the realization of mass storage technology, which effectively solved the problem of mass storage and real-time transmission of data in a deep borehole environment. The effectiveness of the surface-borehole TEM systems with a long wire source and a large loop source was verified by tests in real mines. The surface-borehole electromagnetic signal acquisition system developed in this study effectively collected electromagnetic signals in the borehole, and the results accurately reflected the stratigraphic information of mineral resources in the study area. This study can pave a new technical path for the exploration of deep and peripheral areas of non-ferrous metal mines and provide valuable experience and insights for mineral resource exploration in similarly complex geological environments.
Characterization of Ancient Cereals Cultivated by Intensive and Organic Procedures for Element Content
Authors: Radaelli Marta , Scalabrin Elisa , Roman Marco , Buffa Gabriella , Griffante Irene , Capodaglio Gabriele
Published: August 2024

According to their nutritional value, their ability to adapt to the various environmental conditions, and their versatility, cereals are among the most cultivated plants in the world. However, the ongoing climate changes subject crops to important environmental stress that for some varieties leads to high production losses. Therefore, the selection of species and varieties that are more versatile and adaptable to different environmental conditions can be important. However, the characteristics of some cereals are not completely known; this is a priority before aiming to improve their cultivation. The aim of this study is to characterize select species that are potentially suitable for local environmental conditions and that possess nutritional value. The elemental composition was assessed in different cereal species grown following intensive and organic agriculture practices. Six species were grown for this study with techniques of intensive agriculture: Triticum monococcum L., Triticum dicoccum L., Triticum aestivum L., variety Verna, Triticum durum Desf., variety Senatore Cappelli, Triticum durum Desf., variety Claudio, and Avena strigosa Schreb.; four of these were also grown following organic procedures: Triticum monococcum L., Triticum dicoccum L., Triticum aestivum L., variety Verna, and Triticum durum Desf., variety Senatore Cappelli. The study considered twenty elements, including major nutrients (Ca, K, Mg, P, and S), seven micronutrients (B, Cu, Fe, Mn, Mo, Se, and Zn), and trace elements with toxic properties (Al, Ba, Cd, Cr, Na, Rb, Sc, and Sr) that can be accumulated at the seed level. The results highlight the differences in the element concentrations in the cereal seeds in relation to the genus and species; the highest concentrations of the major nutrients appeared in T. monococcum; the concentrations were 6.9, 2.09, 7.2, and 2.9 mg/g for K, Mg, P, and S, respectively. The highest concentrations of certain micronutrients, B, Ca, Mo, and Se (16, 785, 3.69, and 0.34 μg/g), were in A. strigosa. There is also evidence that the element content can be affected by the adopted cultivation procedure; however, the effects of the growing procedure can be significantly different when different species are considered. T. monococcum, grown by an organic procedure, presented lower concentrations of the major nutrients, while it demonstrated a modest increase in the micronutrients in the T. durum variety organic S. Cappelli, and the production procedure did not affect the elemental composition of the T. aestivum variety Verna. The survey also highlights that the studied species and the growing procedure affected the capacity to accumulate and translocate trace hazardous elements for human health at the seed level.
User Experience in Immersive Virtual Reality-Induced Hypoalgesia in Adults and Children Suffering from Pain Conditions
Authors: Guerra-Armas Javier , Flores-Cortes Mar , Ceniza-Bordallo Guillermo , Matamala-Gomez Marta
Published: August 2024

Pain is the most common reason for medical consultation and use of health care resources. The high socio-economic burden of pain justifies seeking an appropriate therapeutic strategy. Immersive virtual reality (VR) has emerged as a first-line non-pharmacological option for pain management. However, the growing literature has not been accompanied by substantial progress in understanding how VR could reduce the pain experience, with some user experience factors being associated with the hypoalgesic effects of immersive VR. The aim of this review is (i) to summarize the state of the art on the effects of VR on adults and children suffering from pain conditions; (ii) to identify and summarize how mechanisms across immersive VR user experience influence hypoalgesic effects in patients with acute and chronic pain among adults and children. A critical narrative review based on PICOT criteria (P = Patient or Population and Problem; I = Intervention or Indicator; C = O = Outcome; T = Type) was conducted that includes experimental studies or systematic reviews involving studies in experimentally induced pain, acute pain, or chronic pain in adults and children. The results suggest an association between immersive VR-induced hypoalgesia and user experience such as distraction, presence, interactivity, gamification, and virtual embodiment. These findings suggest that hierarchical relationships might exist between user experience-related factors and greater hypoalgesic effects following an immersive VR intervention. This relationship needs to be considered in the design and development of VR-based strategies for pain management.
The Preparation of a Polyamidoxime–Phosphorylated Cellulose Nanofibrils Composite Aerogel for the Selective Extraction of Uranium from Seawater
Authors: Yang Xiaoying , Cui Mei , Su Rongxin , Huang Renliang
Published: August 2024

Uranium is the most important fuel for nuclear power operations, and the safe supply of its resources is the key to the development of nuclear power in China. Because of the complex seawater environment and extremely low uranium concentration, extracting uranium from natural seawater poses a significant challenge. In this study, a polyamidoxime–phosphorylated cellulose nanofibril composite aerogel was prepared as an adsorbent for uranium extraction from seawater. An adsorption kinetics test, equilibrium adsorption isotherm model fitting, an adsorption–desorption cycle test, and a selectivity test were carried out to evaluate the adsorption performance of the composite aerogel for uranium extraction. The adsorption capacities for the initial concentrations of 4 and 8 ppm in uranium-spiked pure water were 96.9 and 204.3 mg-U/g-Ads, respectively. The equilibrium uranium adsorption capacities of uranium-spiked simulated seawater were 38.9 and 51.7 mg-U/g-Ads, respectively. The distribution coefficient KD of uranium was calculated to be 2.5 × 107 mL/g. The results show that the polyamidoxime–phosphorylated cellulose nanofiber composite aerogels prepared in this study have the advantages of low cost and high uranium selectivity for uranium extraction from seawater.
Reviewing Material-Sensitive Computed Tomography: From Handcrafted Algorithms to Modern Deep Learning
Authors: Weiss Moritz , Meisen Tobias
Published: August 2024

Computed tomography (CT) is a widely utilised imaging technique in both clinical and industrial applications. CT scan results, presented as a volume revealing linear attenuation coefficients, are intricately influenced by scan parameters and the sample’s geometry and material composition. Accurately mapping these coefficients to specific materials is a complex task. Traditionally, material decomposition in CT relied on classical algorithms using handcrafted features based on X-ray physics. However, there is a rising trend towards data-driven approaches, particularly deep learning, which offer promising improvements in accuracy and efficiency. This survey explores the transition from classical to data-driven approaches in material-sensitive CT, examining a comprehensive corpus of literature identified through a detailed and reproducible search using Scopus. Our analysis addresses several key research questions: the origin and generation of training datasets, the models and architectures employed, the extent to which deep learning methods reduce the need for domain-specific expertise, and the hardware requirements for training these models. We explore the implications of these findings on the integration of deep learning into CT practices and the potential reduction in the necessity for extensive domain knowledge. In conclusion, this survey highlights a significant shift towards deep learning in material-resolving CT and discusses the challenges and opportunities this presents. The transition suggests a future where data-driven approaches may dominate, offering enhanced precision and robustness in material-resolving CT while potentially transforming the role of domain experts in the field.
Artificial Intelligence as a Replacement for Animal Experiments in Neurology: Potential, Progress, and Challenges
Authors: Rudroff Thorsten
Published: August 2024

Animal experimentation has long been a cornerstone of neurology research, but it faces growing scientific, ethical, and economic challenges. Advances in artificial intelligence (AI) are providing new opportunities to replace animal testing with more human-relevant and efficient methods. This article explores the potential of AI technologies such as brain organoids, computational models, and machine learning to revolutionize neurology research and reduce reliance on animal models. These approaches can better recapitulate human brain physiology, predict drug responses, and uncover novel insights into neurological disorders. They also offer faster, cheaper, and more ethical alternatives to animal experiments. Case studies demonstrate AI’s ability to accelerate drug discovery for Alzheimer’s, predict neurotoxicity, personalize treatments for Parkinson’s, and restore movement in paralysis. While challenges remain in validating and integrating these technologies, the scientific, economic, practical, and moral advantages are driving a paradigm shift towards AI-based, animal-free research in neurology. With continued investment and collaboration across sectors, AI promises to accelerate the development of safer and more effective therapies for neurological conditions while significantly reducing animal use. The path forward requires the ongoing development and validation of these technologies, but a future in which they largely replace animal experiments in neurology appears increasingly likely. This transition heralds a new era of more humane, human-relevant, and innovative brain research.
High-Fat Diet Delays Liver Fibrosis Recovery and Promotes Hepatocarcinogenesis in Rat Liver Cirrhosis Model
Authors: Taguchi Daisuke , Shirakami Yohei , Sakai Hiroyasu , Maeda Toshihide , Miwa Takao , Kubota Masaya , Imai Kenji , Ibuka Takashi , Shimizu Masahito
Published: August 2024

More effective treatments for hepatitis viral infections have led to a reduction in the incidence of liver cirrhosis. A high-fat diet can lead to chronic hepatitis and liver fibrosis, but the effects of lipid intake on liver disease status, including hepatitis C virus and alcohol, after elimination of the cause are unclear. To investigate the effects, we used a rat cirrhosis model and a high-fat diet in this study. Male Wistar rats were administered carbon tetrachloride for 5 weeks. At 12 weeks of age, one group was sacrificed. The remaining rats were divided into four groups according to whether or not they were administered carbon tetrachloride for 5 weeks, and whether they were fed a high-fat diet or control diet. At 12 weeks of age, liver fibrosis became apparent and then improved in the groups where carbon tetrachloride was discontinued, while it worsened in the groups where carbon tetrachloride was continued. Liver fibrosis was notable in both the carbon tetrachloride discontinuation and continuation groups due to the administration of a high-fat diet. In addition, liver precancerous lesions were observed in all groups, and tumor size and multiplicity were higher in the high-fat diet-fed groups. The expression of genes related to inflammation and lipogenesis were upregulated in rats fed a high-fat diet compared to their controls. The results suggest that a high-fat diet worsens liver fibrosis and promotes liver carcinogenesis, presumably through enhanced inflammation and lipogenesis, even after eliminating the underlying cause of liver cirrhosis.
Use of Interactive Food Labels to Increase Confidence in Preparing Produce among College Students
Authors: Spaccarotella Kim , Mishra Sasmita , Healy Liam
Published: August 2024

College students may have limited access to produce and may lack confidence in preparing it, but cooking videos can show how to make healthy dishes. The Cognitive Theory of Multimedia Learning suggests that learning is enhanced when visual and auditory information is presented considering cognitive load (e.g., highlighting important concepts, eliminating extraneous information, and keeping the video brief and conversational). The purpose of this project was to pilot test a food label for produce grown at an urban university and assess whether student confidence in preparing produce improved after using the label and QR code to view a recipe video developed using principles from the Cognitive Theory of Multimedia Learning. The video showed a student preparing a salad with ingredients available on campus. Students indicated the label was helpful and reported greater perceived confidence in preparing lettuce after viewing the label and video (mean confidence of 5.60 ± 1.40 before vs. 6.14 ± 0.89 after, p = 0.016, n = 28). Keeping the video short and providing ingredients and amounts onscreen as text were cited as helpful. Thus, a brief cooking video and interactive label may improve confidence in preparing produce available on campus. Future work should determine whether the label impacts produce consumption and if it varies depending on the type of produce used.
Adherence to a Cholesterol-Lowering Diet and the Risk of Pancreatic Cancer: A Case–Control Study
Authors: Di Maso Matteo , Augustin Livia S. A. , Jenkins David J. A. , Crispo Anna , Toffolutti Federica , Negri Eva , La Vecchia Carlo , Ferraroni Monica , Polesel Jerry
Published: August 2024

Background: Pancreatic cancer risk has been associated with increased serum cholesterol level, which is in turn partially influenced by diet. This study aimed at evaluating the association between pancreatic cancer risk and the adherence to a plant-based cholesterol-lowering diet. Methods: Data were derived from an Italian case–control study including 258 pancreatic cancer patients and 551 controls. The cholesterol-lowering diet score was based on seven components: high intakes of (i) non-cellulosic polysaccharides (a proxy of viscous fibers), (ii) monounsaturated fatty acids, (iii) legumes, and (iv) seeds/corn oils (a proxy of phytosterols); and low intakes of (v) saturated fatty acids, (vi) dietary cholesterol, and (vii) food with a high glycemic index. The score was calculated adding one point for each fulfilled component, thus ranging from zero (no adherence) to seven (complete adherence). The odds ratios (ORs) and 95% confidence intervals (CIs) were estimated through the logistic regression model. Results: Scores 5–7 were associated with reduced cancer risk (OR = 0.30; 95% CI: 0.18–0.52) compared to scores 0–2. Conclusions: Adherence to a plant-based cholesterol-lowering diet was associated with a reduced risk of pancreatic cancer.
Phytochemical Index and the Risk of Gastritis/Gastric Ulcer among Korean Adults: A Prospective Cohort Study
Authors: Park Yeeun , Park Kyong
Published: August 2024

Phytochemicals found in fruits, vegetables, and plant-based foods have potential protective effects against various diseases, including gastric disorders. This study aimed to analyze the longitudinal association between phytochemical intake and the risk of gastritis/gastric ulcer in Korean adults. This was a prospective cohort study, a community-based cohort conducted as part of the Korean Genome and Epidemiology Study, examining the association between phytochemical intake and the risk of gastritis/gastric ulcer in Korean adults. Dietary information was collected using a validated semi-quantitative food frequency questionnaire, and the phytochemical index (PI) was calculated. The study included 7377 Korean men and women aged 40–69 years without gastritis/gastric ulcer at baseline of the Korea Association Resource study in Korea. The incidence of gastritis/gastric ulcer was determined using a survey questionnaire administered by trained staff. Multivariate Cox proportional hazards regression was used to calculate the hazard ratio and 95% confidence interval to determine the association between PI and risk of gastritis/gastric ulcer. During the median follow-up period of 9.50 years, 729 cases were reported. The fully adjusted model showed a significantly lower risk of gastritis/gastric ulcer in the highest PI quartile compared to the lowest (hazard ratio: 0.78, 95% confidence interval: 0.61–0.98), and this association was linear (p for trend = 0.01). This research indicates that incorporating foods abundant in phytochemicals into one’s diet could be associated with a reduced risk of developing gastritis/gastric ulcers. These findings underscore the importance of further investigating the role of phytochemical-rich diets in gastrointestinal health, as demonstrated in this study.
Promoting Glutathione Synthesis: A Possibility for Treating Cardiomyopathy Induced by a Maternal Western Diet
Authors: Zhang Jialing , Wang Jiayu , Xu Da , Gui Yiting , Bai Fan , Huo Yu , Cao Li , Gui Yonghao
Published: August 2024

Background: The adverse effects of a Western diet on obesity and diabetes among reproductive-aged women pose a significant threat to the cardiovascular health of their offspring. Given the crucial role of glutathione metabolism and glutathione-related antioxidant defense systems in cardiovascular diseases through scavenging ROS and maintaining redox homeostasis, further exploration of their specific influence is imperative to develop therapeutic strategies for cardiomyopathy induced by a maternal Western diet. Methods: We developed a prenatal maternal Western diet exposure model in C57/B6 mice to investigate cardiac morphology and function through histological analysis and echocardiography. RNA sequencing and analysis were utilized to elucidate the mechanisms underlying the impact of a maternal Western diet and N-acetylcysteine treatment on cardiomyopathy. Additionally, ELISAs, transmission electron microscopy, and flow cytometry were employed to assess the antioxidant defense system and mitochondrial ROS levels in progenitor cardiomyocytes. Results: N-acetylcysteine significantly mitigated cardiomyocyte hypertrophy, myocardial interstitial fibrosis, collagen type I accumulation, and left ventricular remodeling induced by a maternal Western diet, particularly in male offspring. Furthermore, N-acetylcysteine reversed the increase in apoptosis and the increase in the β/α-MyHC ratio in the myocardium of offspring that results from a maternal Western diet. RNA sequencing and GSEA revealed that the beneficial effects of N-acetylcysteine were linked to its ability to modulate oxidative phosphorylation pathways. Additionally, N-acetylcysteine treatment during pregnancy can markedly elevate glutathione levels, augment glutathione peroxidase (GPx) activity, and mitigate the accumulation of mitochondrial ROS caused by a maternal Western diet. Conclusions: N-acetylcysteine mitigated cardiomyopathy induced by a maternal Western diet by bolstering glutathione synthesis and enhancing GPx activity, thereby scavenging mitochondrial ROS and modulating oxidative phosphorylation pathways.
Acute Cardiovascular Effects of Simultaneous Energy Drink and Alcohol Consumption in Young Adults: A Review of Case Reports
Authors: Azarm Victor , Link Jan-Philipp , Mandilaras Guido , Li Pengzhu , Dalla-Pozza Robert , Jakob André , Haas Nikolaus Alexander , Oberhoffer Felix Sebastian , Schrader Meike
Published: August 2024

(1) Background: The aim of this review was to identify and summarize adverse cardiovascular health events associated with the simultaneous consumption of energy drinks (ED) and alcohol. Potential prevention strategies and the implementation of research toward the underlying mechanisms for these pathologies were highlighted to emphasize the need for further investigation and to encourage more attention to this field. (2) Methods: The PubMed database was searched for case reports linked with adverse cardiovascular events after simultaneous ED and alcohol consumption. Inclusion criteria were: the reported age of the patient is between 16 and 35 years and confirmed co-consumption of EDs and alcohol. All relevant articles that met the inclusion criteria were fully read and all relevant data was extracted. The extracted data was summarized and presented in this review of cases. (3) Results: In total, 10 cases were identified. The analysis showed that mainly young adults (median age = 24.5 years), in particular men (80%) were affected. The three parts of the cardiovascular system affected were heart rhythm (42%), myocardial function (33%), and coronary arteries (25%). In 3 cases the outcome was fatal. Moreover, preexisting health conditions and/or potential trigger factors were present in 60% of selected cases. (4) Conclusions: This review of case reports suggests that the simultaneous consumption of EDs and alcohol can lead to adverse cardiovascular health events and even incidents with fatal outcomes were reported. Potential trigger factors and preexisting health conditions seem to increase the probability of adverse cardiovascular health events. Consumers should be informed about the potential risks and follow responsible consumption behavior to prevent future health events. More systematic studies are needed to determine the acute effects on the cardiovascular system in young adults.
Status Asthmaticus in the Pediatric ICU: A Comprehensive Review of Management and Challenges
Authors: Joseph Amy , Ganatra Hammad
Published: August 2024

This narrative review addresses the significant burden of pediatric status asthmaticus, which comprises almost 20% of admissions to pediatric intensive care units (PICUs). It highlights the diverse modalities employed in the PICU for managing this life-threatening condition, and thoroughly discusses the literature in support of or against these treatment modalities.
A Phytochemical Analysis and the Pharmacological Implications of the Seagrass Halodule uninervis: An Overview
Authors: Wehbe Nadine , Bechelany Mikhael , Badran Adnan , Al-Sawalmih Ali , Mesmar Joelle Edward , Baydoun Elias
Published: August 2024

Seagrasses are marine angiosperms that inhabit tropical and subtropical regions around the world. They play a vital role in marine biodiversity and the ecosystem by providing habitats and food for several marine organisms, stabilizing sediments, and improving water quality. Halodule uninervis from the family Cymodoceaceae has been used in traditional folk medicine for the treatment of many ailments. Additionally, several identified bioactive metabolites have been shown to contribute to its pharmacological activities, including anticancer, anti-inflammatory, and antioxidant. As such, H. uninervis could contribute to the development of novel drugs for various diseases. This review aims to compile the phytochemical composition and pharmacological activities of H. uninervis. Furthermore, details about its botanical characteristics and ecological significance are also discussed. By providing valuable insights into the role of H. uninervis in both the marine ecosystem and biomedicine, this review helps to highlight its potential as a therapeutic agent for future drug discovery and development.
Orodispersible Dosage Forms with Rhinacanthin-Rich Extract as a Convenient Formulation Dedicated to Pediatric Patients
Authors: Suksawat Thongtham , Brniak Witold , Łyszczarz Ewelina , Wesoły Małgorzata , Ciosek-Skibińska Patrycja , Mendyk Aleksander
Published: August 2024

Rhinacanthins, derived from Rhinacanthus nasutus, widely used in traditional medicine, exhibit antifungal, anticancer, antiviral, antibacterial, and antiplatelet aggregation effects. Recently, their anti-diabetic activity was confirmed, which makes them an interesting natural alternative in the therapy of the early stage of diabetes mellitus. The aim of this study was to demonstrate the possibility of formulating orodispersible tablets (ODTs) and orodispersible films (ODFs) containing rhinacanthin-rich extract (RRE). Tablets with 50 mg or 100 mg of RRE were produced by direct compression. ODFs were manufactured by casting of Lycoat RS 720 or polyvinyl alcohol solution with RRE and additional excipients. The mechanical properties and disintegration times of the prepared formulations were studied. The effectiveness of taste masking was analyzed with an electronic tongue system. Six months simplified stability studies were performed in conditions complying to ICH guidelines. Appropriate friability of ODTs was achieved, despite low tensile strength (0.45–0.62 MPa). All prepared ODFs successfully met the acceptance criteria regarding Young’s modulus, tensile strength, and elongation at break. The observed variations in their mechanical properties were dependent on the type and quantity of polymers and plasticizers used. Disintegration time of ODTs ranged from 38.7 s to 54.2 s, while for ODFs from 24.2 to 40 s in the pharmacopoeial apparatus. Analyses made with the electronic tongue showed the significant taste-masking effect in both formulations. The addition of sucralose as a sweetener and menthol with mint flavor as a taste-masking agent was sufficient to mask an RRE’s taste in the case of ODTs and ODFs. Stability studies of ODTs packed in the PVC/Alu blisters showed a decrease in the RRE content below 90% after 6 months. However, ODFs with PVA were physicochemically stable for 6 months while being stored in Alu/Alu sachets. Our study proved for the first time the possibility of the formulation of orodispersible dosage forms with RRE, characterized by good mechanical properties, disintegration time, and appropriate taste masking.
Mechanism, Formulation, and Efficacy Evaluation of Natural Products for Skin Pigmentation Treatment
Authors: Peng Xueli , Ma Yuning , Yan Chenxin , Wei Xiaocen , Zhang Linlin , Jiang Hehe , Ma Yuxia , Zhang Suohui , Xing Mengzhen , Gao Yunhua
Published: August 2024

Skin pigmentation typically arises from the excessive secretion and accumulation of melanin, resulting in a darker complexion compared to normal skin. Currently, the local application of chemical drugs is a first-line strategy for pigmentation disorders, but the safety and efficacy of drugs still cannot meet clinical treatment needs. For long-term and safe medication, researchers have paid attention to natural products with higher biocompatibility. This article begins by examining the pathogenesis and treatment approaches of skin pigmentation diseases and summarizes the research progress and mechanism of natural products with lightening or whitening effects that are clinically common or experimentally proven. Moreover, we outline the novel formulations of natural products in treating pigmentation disorders, including liposomes, nanoparticles, microemulsions, microneedles, and tocosomes. Finally, the pharmacodynamic evaluation methods in the study of pigmentation disorder were first systematically analyzed. In brief, this review aims to collect natural products for skin pigmentation treatment and investigate their formulation design and efficacy evaluation to provide insights for the development of new products for this complex skin disease.
Polyvinyl Alcohol Coatings Containing Lamellar Solids with Antimicrobial Activity
Authors: Bastianini Maria , Sisani Michele , Escudero García Raúl , Di Guida Irene , Russo Carla , Pietrella Donatella , Narducci Riccardo
Published: August 2024

The design of an antimicrobial coating material has become important in the prevention of infections caused by the transmission of pathogens coming from human contact with contaminated surfaces. With that aim, layered single hydroxides (LSHs) and layered double hydroxides (LDHs) containing Zn and Cu intercalated with antimicrobial molecules were synthesized and characterized. Cinnamate and salicylate anions were chosen because of their well-known antimicrobial activity. Several coatings based on polyvinyl alcohol (PVA) and LDHs or LSHs with increasing amounts of filler were prepared and filmed on a polyethylene terephthalate (PET) substrate. The coatings were characterized, and their antimicrobial activity was evaluated against several pathogens that are critical in nosocomial infections, showing a synergistic effect between metal ions and active molecules and the ability to inhibit their growth.
The Impact of Protein in Post-Menopausal Women on Muscle Mass and Strength: A Narrative Review
Authors: Black Katherine Elizabeth , Matkin-Hussey Penelope
Published: August 2024

Background: Menopause is a significant period in the life of a female; many hormonal and lifestyle changes occur, which can have a catastrophic effect on their health and well-being. Amongst these changes is the loss of muscle mass and strength. Resistance training is recommended for post-menopausal women; however, the role of protein in muscle mass and strength in this population is unclear. Methods: This narrative review discusses the research evidence regarding daily protein needs, dose and timings of intake, and protein quality. Results: Observational and interventional studies suggest post-menopausal females should ingest at least the RDA 0.8 g·kg−1·d−1 of protein, the dosing at each meal maybe important. Both whey and soy protein may provide some benefit to muscle strength. Conclusions: Overall, there is limited evidence and not of high quality, making it difficult to make inferences about the protein needs of post-menopausal females.
Chemical and Protein Characterization of Two Varieties of Chickpea (Cicer Arietinum): Costa 2004 and El Patrón
Authors: Pascual-Bustamante Selene , Raya-Pérez Juan Carlos , Aguirre-Mancilla César Leobardo , Ramírez Pimentel Juan Gabriel , Vargas-Martínez María Gabriela , Trejo-Márquez María Andrea
Published: August 2024

The objective of this study was to evaluate the chemical composition of two chickpea varieties, ‘Costa 2004’ and ‘El Patrón’, and to characterize their proteins to determine their technological potential for the food industry. For this purpose, chickpea samples of both varieties from the 2019 harvest region of Guanajuato, Mexico, were obtained and chemically characterized to determine the protein fractions using electrophoretic and amino acid profiling. The chickpea variety ‘Costa 2004’ contained 3% less protein and 7% less dietary fiber content than the variety ‘El Patrón’; whereas, the carbohydrate content of ‘Costa 2004’ was 4% greater. Additionally, the chickpeas demonstrated an antioxidant capacity ranging from 319 to 387 µMET/g and total phenol levels exceeding 500 mg/g. Among the protein fractions, globulins represented the highest proportion in both varieties of chickpea, at approximately 8.73 g/100 g (‘Costa 2004’) and 10.42 g/100 g (‘El Patrón’), followed by albumin, at approximately 1.24 g/100 g and 1.47 g/100 g, respectively. The chickpea proteins ranged in molecular weight between 100 and 25 kDa, with particularly strong signals in the albumin and globulin bands. Regarding the amino acid profile, histidine was predominant in both varieties. In conclusion, both varieties of chickpea have high nutritional value and broad potential for technological use in the food industry.
Phosphorous Utilization in Microalgae: Physiological Aspects and Applied Implications
Authors: Bossa Rosanna , Di Colandrea Melania , Salbitani Giovanna , Carfagna Simona
Published: August 2024

Phosphorus (P) is a fundamental element for life, playing an integral role in cellular metabolism including energy transfer, nucleic acid synthesis, and membrane structure. This nutrient is critical to the physiological ecology in all photosynthetic organisms including eukaryotic microalgae and cyanobacteria. The review, here presented, delves into the intricate mechanisms governing phosphorus acquisition from the environment, its utilization in plant metabolism, and regulation in these photosynthetic microorganisms. Furthermore, it comprehensively explores the strategies employed by microalgae to cope with phosphorus limitation, such as the activation of high-affinity phosphate transporters and the synthesis of phosphorus storage compounds. On the other hand, the ability to consume abundant phosphate makes microalgae exploitable organisms for environmental remediation processes. The knowledge synthesized in this review contributes to the broader understanding of microalgal physiology, offering insights into the ecological and biotechnological implications of phosphorus assimilation in these microorganisms.
Current Status and Prospects of Pine Wilt Disease Management with Phytochemicals—A Review
Authors: Zhang Quanhong , Si Guiling , Chen Liusheng , Hu Lili , Cui Gaofeng , Wang Min , Zhao Danyang
Published: August 2024

PWD (pine wilt disease) is a devastating forest disease caused by the Bursaphelenchus xylophilus, which is the major invasive species in Asian and European countries. To control this disease, fumigation, pesticide injection, and clear cutting of epidemic trees have been widely used. But these management strategies have many limitations in terms of the effectiveness and environmental impacts, especially for the overuse of chemical pesticides. Thus, PCs (phytochemicals), the various compounds extracted from plants, have drawn extensive attention owing to their special characteristics, including abundant sources, low toxicity, high efficacy, and easy degradation. This review provides an overview of the current status of using PCs as alternative approaches to manage PWD. It discusses the efficacy of various PCs, the factors influencing their nematicidal activity, and their mechanism of action against B. xylophilus. These results will reveal the application of PCs in combating these devastating diseases and the necessity for further research.
Establishment of Novel Simple Sequence Repeat (SSR) Markers from Chimonanthus praecox Transcriptome Data and Their Application in the Identification of Varieties
Authors: Liu Bin , Wu Hua-Feng , Cao Yin-Zhu , Yang Xi-Meng , Sui Shun-Zhao
Published: August 2024

Chimonanthus praecox, a member of the Calycanthaceae family, is a unique, traditional, and famous flowering economic tree species in China. Despite the existence of several varieties, only a few cultivars have been formally named. Currently, expression sequence tag–simple sequence repeat (EST-SSR) markers are extensively used to identify different species and varieties; a large number of microsatellites can be identified from transcriptome databases. A total of 162,638 unigenes were assembled using RNA-seq; 82,778 unigenes were annotated using the Nr, Nt, Swiss-Prot, Pfam, GO, KOG, and KEGG databases. In total, 13,556 SSR loci were detected from 11,691 unigenes, with trinucleotide repeat motifs being the most abundant among the six repeat motifs. To develop the markers, 64,440 pairs of SSR primers with polymorphism potential were designed, and 75 pairs of primers were randomly selected for amplification. Among these markers, seven pairs produced amplified fragments of the expected size with high polymorphism. Using these markers, 12 C. praecox varieties were clustered into two monophyletic clades. Microsatellites in the transcriptome of C. praecox exhibit rich types, strong specificity, and great polymorphism potential. These EST-SSR markers serve as molecular technical methods for identifying different varieties of C. praecox and facilitate the exploration of a large number of candidate genes associated with important traits.
Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Varieties from South China
Authors: Tang Liqun , Song Jian , Cui Yongtao , Fan Honghuan , Wang Jianjun
Published: August 2024

Rice blast caused by the pathogenic fungus Magnaporthe oryzae poses a significant threat to rice cultivation. The identification of robust resistance germplasm is crucial for breeding resistant varieties. In this study, we employed functional molecular markers for 10 rice blast resistance genes, namely Pi1, Pi2, Pi5, Pi9, Pia, Pid2, Pid3, Pigm, Pikh, and Pita, to assess blast resistance across 91 indica rice backbone varieties in South China. The results showed a spectrum of resistance levels ranging from highly resistant (HR) to highly susceptible (HS), with corresponding frequencies of 0, 19, 40, 27, 5, and 0, respectively. Yearly correlations in blast resistance genes among the 91 key indica rice progenitors revealed Pid2 (60.44%), Pia (50.55%), Pita (45.05%), Pi2 (32.97%), Pikh (4.4%), Pigm (2.2%), Pi9 (2.2%), and Pi1 (1.1%). Significant variations were observed in the distribution frequencies of these 10 resistance genes among these progenitors across different provinces. Furthermore, as the number of aggregated resistance genes increased, parental resistance levels correspondingly improved, though the efficacy of different gene combinations varied significantly. This study provides the initial steps toward strategically distributing varieties of resistant indica rice genotypes across South China.
Development of Apomictic 56-Chromosomal Maize–Tripsacum Hybrids: A Potential Breakthrough in Heterosis Fixation
Authors: Sokolov Viktor Andreevich , Panikhin Pavel Alexandrovich , Plotnikov Kirill Olegovich , Chepurnov Grigory Yurievich , Blinov Alexander Genadievich
Published: August 2024

Maize (Zea mays L.) is one of the most demanded grain crops in the world. Currently, production has exceeded one billion tons and is increasing by 3–5% annually. Such growth is due to the genetic potential of the crop and the use of heterosis F1 hybrids in production. However, the need to produce first-generation seed annually poses significant challenges and is an economically costly technology. A solution to this problem may be the transfer of the asexual (apomictic) mode of reproduction to maize from its wild relative, eastern gamagrass (Tripsacum dactyloides L.). In this work, we report the production of 56-chromosome apomictic hybrids of maize (Zea mays L.) with eastern gamagrass (T. dactyloides L.) with restored anther fertility. The mode of reproduction of the plant was confirmed by counting chromosomes and sequencing the nuclear gene (Pox3) and chloroplast tRNA-Leu (trnL) gene. These apomictic hybrids had karyotypes of 2n = 56 = [(10Zm(573MB) + 36Td) + 10Zm(611CB)] and 2n = 56 = [(10Zm(611CB) + 36Td) + 10Zm(611CB)]. The resulting hybrids can be widely used as a fodder crop.
Hybrid Dielectric Barrier Discharge Reactor: Characterization for Ozone Production
Authors: Korzec Dariusz , Freund Florian , Bäuml Christian , Penzkofer Patrik , Nettesheim Stefan
Published: August 2024

The generation of ozone by dielectric barrier discharge (DBD) is widely used for water and wastewater treatment, the control of catalytic reactions, and surface treatment. Recently, a need for compact, effective, and economical ozone and reactive oxygen–nitrogen species (RONS) generators for medical, biological, and agricultural applications has been observed. In this study, a novel hybrid DBD (HDBD) reactor fulfilling such requirements is presented. Its structured high-voltage (HV) electrode allows for the ignition of both the surface and volume microdischarges contributing to plasma generation. A Peltier module cooling of the dielectric barrier, made of alumina, allows for the efficient control of plasma chemistry. The typical electrical power consumption of this device is below 30 W. The operation frequency of the DBD driver oscillating in the auto-resonance mode is from 20 to 40 kHz. The specific energy input (SEI) of the reactor was controlled by the DBD driver input voltage in the range from 10.5 to 18.0 V, the Peltier current from 0 to 4.5 A, the duty cycle of the pulse-width modulated (PWM) power varied from 0 to 100%, and the gas flow from 0.5 to 10 SLM. The operation with oxygen, synthetic air, and compressed dry air (CDA) was characterized. The ultraviolet light (UV) absorption technique was implemented for the measurement of the ozone concentration. The higher harmonics of the discharge current observed in the frequency range of 5 to 50 MHz were used for monitoring the discharge net power.