bluuebunny/crossref_metadata_2025 huggingface dataset:
Структурно-функциональные особенности ландшафтов с активным термокарстом в северной тайге Западной Сибири
Authors: Lim A. G. , Loiko S. V. , Kritckov I. V. , Kuzmina D. M.
Published: January 2018

In the article, the main results of the study of dissolved greenhouse gases (CO2, CH4), dissolved organic carbon (DOC) and colloidal trace elements-metals in surface and soil waters of hollows, depressions, permafrost subsidences are considered. These waters were sampled in areas with active and stable permafrost thawing in Western Siberia. It is found that these zones of active termokarst are mainly located on the slopes and the bottoms of the upper reaches of rivers, which drain the interfluve. Active thawing of flat mound bogs leads to the appearance of numerous hollows, depressions and thermokarst subsidences, especially on the border between forest and fens. This is related to the additional heat input from the flowing water and causing the thawing deep permafrost on the edges of ridges occupied by the forest. Increase in specific conductivity (S.C.) in the smallest water bodies and supra-permafrost water is accompanied by a significant increase in concentrations of DOC, P, K, Cu, Zn, Mo and Ba. It has been found that small hollows and subsidence (< 10 – 100 м²) are characterized by much higher concentrations of DOC and CO2 (p < 0.05) in comparison with large depressions and thermokarst water bodies. There is also a decrease of CH4 concentration with an increase in the size of water bodies (p < 0.05), despite the fact that the data dispersion reaches 3 orders in small (<100 – 300 m²) water bodies of the where located zones active termokarst landscape, concentrations of DOC, CO2 and CH4 are significantly higher (p <0.05) than in the previously studied water bodies formed under conditions of a stable state of permafrost in areas of Nojabrsk and Khanymey. Waters of the active thawed of permafrost peat landscapes are usually rich in oxygen (saturation in the range of 30 ≤ O2 ≤ 105 %) and approximately 0.5 unit’s pH more acidic than areas with a stable state of permafrost. Many major and trace elements show a statistically significant decrease in concentration from very small water bodies (< 10 m²) to larger thermokarst waters. By the results of carried out research we can say that in the landscapes with active termokarst there will be direct mobilization of soil water in the hydrological network (rivers and lakes) with a minimal transformation of the water composition within the catchment area.
Do we have infraspecific taxa of Salvia multicaulis Vahl. (Lamiaceae) in Iran?
Authors: Talebi Seyed Mehdi , Rezakhanlou Reza , Matsyura A V.
Published: January 2018

Salvia multicaulis is a widespread species of Lamiaceae family in Iran. There are many discussions about its infraspecific variations. Although some varieties were definite for this species in various parts of the world, no infraspecific taxon was reported in Iran and all samples of this species were named as S. multicaulis. In this study, morphological characteristics of S. multicaulis populations, naturally growing in Iran, was examined. Twenty-two traits were examined in 94 individuals of this species to
identify their phenotypic difference. Most of the investigated features were showing a high degree of variability, but it was highly pronounced for some characteristics such as basal leaf shape, basal leaf width, basal leaf length/ width ratio and basal petiole length. Significant positive/negative correlations were observed between some morphological variables. Furthermore, significant negative correlations were found between the eastern distribution of populations with basal leaf petiole length and basal leaf length/ width ratio. Based on the UPGMA cluster analysis, populations were divided into two main branches. The first branch contained four populations, while the second branch was bigger and clustered in two sub-branches. In one of them,
three populations and in another one the rest populations arranged in two groups. CA joined plot confirmed that each of studied populations or group of populations had distinct morphological trait(s), which were useful in identification of them. Our findings supported population no. 13 had unique morphological traits such as the largest bracts and basal leaf petiole, highest flower number of each inflorescence cycle, widest and largest calyx. The conservation of the highly diverse populations of
Iranian S. multicaulis is recommended.
Estuarine crabs of the Okhotsk Sea and the Sea of Japan: rare species and new records
Authors: Marin I. N.
Published: January 2018

The paper presents the list of coastal estuarine crabs occurring in estuaries of rivers flowing into the southern part of the Okhotsk Sea and Russian coasts of the Sea of Japan. At the moment the list includes five species: Hemigrapsus penicillatus (de Haan, 1835) (Varunidae) (first satisfied record for the Russian fauna), Hemigrapsus takanoi Asakura and Watanabe, 2005 (Varunidae), Eriocheir japonica (de Haan, 1835) (Varunidae), Helice tridens (de Haan, 1835) (Varunidae) (recorded as extinct species for the area) and Deiratonotus cristatum (de Man, 1895) (Camptandriidae). Two species, H. takanoi and E. japonica, are widespread along the Russian coast of the Sea of Japan and in the southern part of the Okhotsk Sea. Helice tridens previously recorded the region by the literature as “widespread” is probably presently extinct in the studied area as not recorded since 2000-ies. Two remaining species, D. cristatum and H. penicillatus, are marked as restricted-range species: three populations of D. cristatum are known from the estuary of Amur river, the Nevelskiy Strait (the most northern part of the Sea of Japan) and the Salmon Bay of Sakhalin Island (southern part of Okhotsk Sea) respectively whereas the only confirmed habitat of H. penicillatus in the Russian fauna is the estuary Volchanka River in Vostok Bay of the Sea of Japan. Presented records are the most northern known populations of all listed species while the areas of their distribution of them are mostly located in a subtropical region of the Sea of Japan and east coast of the Korean Peninsula south to tropical waters. The paper describes the morphology, coloration, features of ecology and distribution of all estuarine crab species on the territory of Russia and adjacent areas.
The invaders as natural biondicators using the example invasive amphipod Gmelinoides fasciatus (Stebbing, 1899) in the Moscow River Basin: the suggestion of G. lacustris/G. fasciatus bioindication pair
Authors: Marin I. N.
Published: January 2018

The article presents data on the study of the diversity of crustacean communities in the Moscow-river basin. Two most common are two species of amphipods, invasive species Gmelinodes fasciatus and the native species Gammarus lacustris, were detected and studies during the study. However, the number of one of the most important bioindicator species of crustaceans, Asellus aquaticus found in our samples is insufficient for quantitative conclusions. At the same time, population of G. fasciatus can be characterized as stable at all studied stations and the species can be used as bioindicator of the natural condition of the river flowing within the Moscow City. The species seems to be significantly less sensitive to environment conditions and especially anthropogenic pollution than the native amphipod species G. lacustris and can successfully replace A. aquaticus as bioindicator species in the area where the latter species is absent. Thus, a new bioindicator pair Gammarus lacustris / Gmelinoides fasciatus is suggested for using in estimation water pollution In European part of the Russian Federation and adjacent areas.
Первая информация о булавоусых чешуекрылых (Insecta, Lepidoptera, Papilionoidea) национального парка ‘Сайлюгемский’ (Юго-Восточный Алтай)
Authors: Naydenov A. E.
Published: January 2018

20 Lepidoptera species from 6 families of Papilionoidea were reported for the territory of the national park ‘Saylyugemsky’. This list is the primary data on the fauna of Lepidoptera in this protected area.
The record of new for the Russian fauna stygobiotic amphipod family Typhlogammaridae (Crustacea) in august cave in Sochi, Krasnodar Region
Authors: Turbanov I. S. , Marin I. N.
Published: January 2018

The record of the representative of the new for Russian fauna stygobiotic amphipod genus Zenkevitchia and family Typhlogammaridae (Crustacea: Amphipoda) discovered in the cave August, Ahshtyr karst massif (Sochi, Krasnodar Territory) is reported. This report significantly expands the range of the family Typhlogammaridae to the northwest along the south-western slope of the Great Caucasus. Besides, the paper provides an overview of all Caucasian representatives of the family Typhlogammaridae and represent the map of their distribution in the region. The taxonomic status of stygobiotic amphipod genus Zenkevitchia is discussed, and it is suggested that the species discovered by us is a new for science and refers to the "admirabilis"-complex of cryptic species (Z. admirabilis, Z. yakovi) inhabiting the territory of Abkhazia. However, the taxonomic description of Zenkevitchia sp. will be carried out in a separate publication on the basis of an integrative taxonomy using morphological and molecular genetic methods.
New records of Lycaenidae and Nymphalidae (Lepidoptera, Papilionoidea) from Altai Mountains
Authors: Yakovlev R. V. , Huemer P. , Wiesmair B. , Sinev S. Yu. , Wieser Ch. , Kulak A. V. , Inozemzev A. G. , Dorozhkin V. V. , Naydenov A. E.
Published: January 2018

For the first time Agriades glandon (de Prunner, 1798) and Issoria eugenia (Eversmann, 1847) are reported from Altai region (Altai Krai, Russian Federation). We also specify the distribution of Lycaena violacea (Staudinger, 1892), Boloria aquilonaris roddi Kosterin, 2000 and Clossiana angarensis (Erschoff, 1870) in the Altai Republic (Russian Federation), Glaucopsyche argali (Elwes, 1899) and Palaeophylotes svetlana (Yakovlev, 2003) on the Mongolian Altai territory (Mongolia, Khovd aimak, Dzun-Dzhargalant-Khairkhan and Bajtag-Bogd-Uul Mountains Ranges).
The first data on many-plumed moths of Namibia (Lepidoptera, Alucitidae)
Authors: Ustjuzhanin P. A. , Kovtunovich V. N.
Published: January 2018

The article gives the first data on the many-plumed moths of Namibia. Two species are reported: Alucita certifica and Alucita homotrocha, previously known from the Republic of South Africa and Zimbabwe respectively
Effects of microbicide based on lactic acid and metal nanoparticles on laboratory animals
Authors: Ponomarenko G. V. , Kovalenko V. L. , Ponomarenko O. V. , Balackiy Yu. O.
Published: January 2018

Modern complex disinfectants should not only be highly effective against most pathogens, but also safe for the body of laboratory and farm animals. To determine the effect of microbicides on animal organism, there are several commonly accepted parameters that are regulated by relevant methodological documents. Taking into account the urgency of the development and implementation of modern disinfectants, the purpose of the researche was to study the effects of the drug "Orgsept" on the indicators of acute toxicity and its harmful effects on laboratory animals. The determination of acute toxicity, the study of the cumulative and skin-resorptive action of the drug "Orgasept" was performed on clinically healthy white mice, and the irritating effect and sensitizing properties were studied on Guinea pigs. The studies were conducted using generally accepted techniques (Yakubchak et al., 2005). According to the results of the research, acute toxicity, cumulative, sensitizing, irritating, skin-resorptive action of the disinfectant based on lactic acid and nanoparticles of silver "Orgasept" on laboratory animals were determined. It has been determined that the investigated preparation according to sanitary-and-hygienic norms GOST 12.1.007-76 belongs to the 3 groups of toxicity in concentrations which are significantly higher than bactericidal, it does not have a pronounced cumulative, sensitizing and skin-resorptive action.
Біополімери сполучної тканини сироватки крові в коней за отруєння чорнокоренем лікарським (Cynoglоssum officinаle)
Authors: Kibkalo D. V. , Tymoshenko O. P. , Borovkov S. B. , Zakharev A. V. , Skripova K. V.
Published: January 2018

The results of a complex study of the effect of Cynoglossum officinale on the organism of horses were presented in the article. The research was conducted in the period from 2014 to 2017 at the Derkulsky stud farm in Lugansk region. It has been established that poisoning by Cynoglossum officinale causes a violation of the hepatotoxic biliary system and the development of cirrhosis in horses. A set of biochemical parameters of blood serum that may be markers of dystrophic processes in liver tissues was determined. The most informative indicators for diagnosis of cirrhosis of the liver in horses are indicators that characterize the state of the connective tissue, namely the content of glycosaminoglycans and their fractions. There was a tendency to increase the number of general GAGs in the horses which died, compared to the control group. In animals that survived, this indicator increased significantly (p≤0.05) compared to the control, indicating the development of fibrous changes in the tissues of parenchymal organs. After analysis of the level of separate GAGs fractions, it was determined that level I of GAGs fraction in all groups was higher than the reference norm, indicating a metabolic abnormality of GAGs of the connective tissue, in particular chondroitin-6-sulfate, especially in animals that survived after poisoning (p≤0.05). Level II of GAGs fraction for most of the horses which died after Cynoglossum officinale poisoning was also higher than the norm: 3.12 ± 0.49 un., and in the horses that survived this figure was 2.67 ± 0,23 un. Consequently, after Cynoglossum officinale poisoning the formation of cholesterol-4 and dermatan sulfates increased that is usually observed in the degenerative processes in the liver and is accompanied with the growth of the connective tissue. Level III of GAGs fraction, the major part of which is heparan and keratan sulfates, increased by 2.6 times (p≤0.05) in case of death of animals after Cynoglossum officinale poisoning and by 2.4 times (p≤0.05) in the horses that survived. It is possible that depolymerization of heparan sulfate occurs in the stromal elements of the liver and of other internal organs which is replaced by chondroitin-4-sulfates as a result of poisoning of the animals by Cynoglossum officinale while Ito cells are transforming into myofibroblasts. These data are confirmed by morphological studies of the liver, which are manifested by dystrophic changes, on the background of cell cytology, hepatodepressive syndrome and an increase in the volume of connective tissue which leads to the death of most of the horses and to the development of chronic pathological process in animals that survive.
Изменение микробиоты кишечника кур в онтогенезе
Authors: Nikonov I. N. , Il'ina L. A. , Kochish I. I. , Romanov M. N. , Podobed L. I. , Laptev G. Yu. , Panin A. N. , Smolenskij V. I. , Suraj P. F.
Published: January 2018

This paper presents the results of a molecular genetic analysis of the changes in the composition of the microbiota of the blind processes of the intestine of the hens of the industrial loam "Lohmann Brown" during ontogeny. According to the results of the analysis of taxonomic affiliation it is established that over 70% of the phylotypes belong to the three phylums - Firmicutes, Bacteroidetes and Proteobacteria, less represented were Actinobacteria, Tenericutes and Fusobacteria, and a significant number of unidentified bacteria was detected. During ontogenesis, birds exhibited marked changes in the ratio of the number of phylotypes and taxonomic groups of the intestinal microbiota. At the age of 20-40 weeks, the birds showed a significant increase in the representatives of the Clostridia class involved in the metabolism of carbohydrates, acid-utilizing bacteria of the order Negativicutes and bacteria with high antagonistic properties (Bifidobacteriales, Bacillus), as well as a significant decrease in the content of a number of opportunistic and pathogenic taxa - family Enterobacteriaceae, the order of Pseudomonadales, phylum Tenericutes. The greatest homogeneity of the bacterial community of the blind processes of the gastrointestinal tract in laying hens was revealed at the age of 20 weeks, which is confirmed by the estimation of biodiversity by means of ecological indices. 
Формування продуктивності сорго цукрового під впливом строків сівби
Authors: Grabovskiy M. B. , Grabovskaya T. O. , Kozak L. A. , Gorodetskyi O. S. , Bohatyr L. V.
Published: January 2018

The scientifically based choice of sowing terms for sorghum depends on the climatic conditions, type, state and humidity of the soil, biological characteristics of varieties and hybrids, the economic purpose of sowing, rate of positive temperatures growth. The purpose of the research was to determine the effect of sowing terms on the growth, development and the yield of green mass of sugar sorghum. In the experiment, the variety of sugar sorghum Silosne 42 and the hybrid Dovista were sown in three terms when the soil temperatures at the depth of seeding were: І – 6–8 °С, ІІ – 8–10 °С, ІІІ – 10–12 °С. Biometric indexes of sugar sorghum depended both on sowing terms and varietal characteristics. The plants most intensively developed and formed the above-ground mass in a variant where the soil temperature at the depth of seeding was 10–12 °С, and plants height was 151.7 and 193.7 cm in the period of flowering – respectively in the Silosne 42 variety and the Dovista hybrid. The highest indexes of the leaf area (51.87 and 57.25 thousand m2/ha), photosynthetic potential (4.26 and 4.32 million m2×days/ha) and the net productivity of photosynthesis (3.50 and 3.54 g/m2 per day) of sugar sorghum were on varieties, sown when the soil temperature at the depth of seeding was 10–12 °С, respectively in the Silosne 42 variety and the Dovista hybrid. It was noted that the increase in the surface of leaf area and the duration of vegetation result in higher photosynthetic potential and, consequently, the growth of the green mass yield. Maximum indexes of pure productivity of photosynthesis are observed in the flowering period, that is, during the period of the most intense growth of the above-ground mass and the leaf surface of sugar sorghum. From the flag leaf stage until flowering stage there is an intense growth in sugar sorghum, and increment of green mass is 57.3-78.6% compared with the growing point differentiation stage. The sorghum biomass indexes had the highest values at all stages of the third sowing term. Increment of the green mass continues to the hard dough stage, while dry matter grows throughout the growing period. The highest yield of the green and dry mass of the Silosne 42 variety and the Dovista hybrid is observed in the hard dough stage, when the soil temperature at the depth of seeding was 10–12 °С – 74.8 and 17.4 t/ha and 83.7 and 20.2 t/ha. The Dovista hybrid prevailed on the yield of the green mass on 9.8–12.7% and dry matter on 4.5–7.8% than the Siloshne 42 variety.
Impact of mulch tillage and fertilization on growth and development of winter wheat plants in clean fallow in Northern Steppe of Ukraine
Authors: Tsyliuryk A. I. , Tkalich Yu. I. , Masliiov S. V. , Kozechko V. I.
Published: January 2018

In conditions of the northern steppe of Ukraine, while cultivating winter wheat after corn in clean fallow the expediency of shallow mulch tillage (disk, flat-cut (early fallow)) has been justified, which provides obtaining equivalent biometric indicators of plant growth and development, grain yield compared to the fall-plowing (the yield of grains for plowing is 5,24-5,50 t/ha, the disking is 5,17-5,60 t/ha, flat-topping loosening (early fallow) – 5,04-5,52 t/ha) on the organic and mineral background (stubble residues + N30-60P30K30). The autumn disk and spring flat-cut cultivation of the fallow field on the background of applying N30-60P30K30 ensure the annual yield of high quality grain of the 3d class (protein – 11,6-12,3%, gluten – 22,6-24,6%), and also contribute to fuel economy during soil cultivation – 22-29 l/ha and guarantee high profitability of grain production at the level of 110-123%).
Control of soil weediness and sugar beets by after crop green manure and different tillages
Authors: Mishchenko Yu. G. , Masik I. M.
Published: January 2018

The research program envisaged the studying of the variation of weediness of sugar beet crops under the influence of after crop sideration and the application of soil protection cultivation. The application of oil radish green manure provided a significant decrease in the amount of seed weeds in the entire 0-30cm layer of soil in comparison with the non-seeded background on 2.8-3.4 mln. seeds ha-1 and a significant decrease in the weed density of all biological groups weeds on 0.5-6.5 weeds m-2 and their weight on 5.6-77.4g m-2 for the period of growing sugar beets. Comparing to plowing the methods of mouldboard-free cultivation provided a significant increase in the quantity of weed seeds in the layer of soil 0-10cm to 18.3-22.9×106 seeds ha-1 and their significant decrease deeper than 10cm on 19.0-23.3 mln. seeds ha-1. Among the variants of mouldboard-free cultivation the lowest amount of weed seeds in the soil layer of 0-5cm was discounted at the deepest cultivation – 24.5 and 23.5×106 seeds ha-1. The decrease of the depth of the mouldboard-free cultivation led to a significant increase in the weed density (by 4.8-68.2 weeds m-2) and their weight (82.5-1193.5g m-2) in sugar beet crops and a substantial shortfall in yields of root crops – 2.9-5.8t ha-1. It follows from the research results that the application of after crop siderate of radish oil and basic mouldboard-free cultivation reduce the potential amount of weed seeds of root layer 0-30 сm; the deep soil cultivation on 28-30cm at the siderate background provides the greatest decrease in real weediness of sugar beet crops and allows to receive high yields of crops – 34.3 t ha-1.
Вплив різного виду підстилки та конструкційних характеристик приміщень на комфорт і поведінку корів
Authors: Borshch O. O. , Borshch O. V. , Kosior L. T. , Pirova L. V. , Lastovska I. O.
Published: January 2018

The article presents the results of investigations of the influence of different types and variants of application of litter material (chopped straw, sawdust, not shredded straw and deep straw bedding) on the comfort of the conditions of keeping and reacting the daily behavior of dairy cows. It is established that the most comfortable for rest among the investigated variants is the maintenance on a deep long-changeable substrate. Under such a maintenance technique, the indicators of the length of rest in the lying position and the number of cows that feed the feed immediately after it was distributed and the milking prevails these indicators obtained by other technologies, since animals are not limited in time and within the recreation areas and have a slightly larger feeding front, which directly influences the processes of milk production. It was revealed that for keeping cows on deep, long-swaddling litter, the smallest number of cases of humidity of resting places, in comparison with other variants of litter. This maintenance technology ensures the uniform application of chopped straw in the range of 6-10 kg per cow throughout the area of the resting place. It was established that by the complex indices, which characterize the comfort of the contents, hygienic assessment and the position of the tibia, the best indicators were for the maintenance of cows on deep litter. For this type of litter animals differed in less pollution of the sides, udder and limbs in comparison with other variants.
Вплив змішанолігандного комплексу кобальту на його обмін у організмі високопродуктивних корів
Authors: Smetanina O. V. , Ibatulin I. I. , Bomko V. S. , Bomko L. G. , Kuzmenko O. A.
Published: January 2018

In the scientific and economic research we investigated the influence different levels of mixed ligand complex of Cobalt on milk productivity and its exchange in the organism of highly productive cows of Ukrainian Black-and-White milk breed in combination with sulfates: Zinc 650 g/t, potassium 38 g/t, sodium selenite 1.8 g/t. For cows of the 1st control group, we administrated 8.9 g/t of cobalt sulfate, 2nd group – 9.7 g/t of mixed ligand complex of cobalt, 3rd, 4th and 5th experimental groups respectively, were fed into rations, g/t: 7.3; 4.9, and 2.4. As a result, the cows of the 1st control group and the 2nd experimental group the deficit in Cobalt was eliminated 100 % to the current norm, and for cows of the 3rd, 4th and 5th experimental groups - 75, 50 and 25 %.
The conducted researches have proved that the highest average daily milk yield of natural fat had cows of the 3rd experimental group, which consumed mixed fodder in the composition of mixed ligand complex of Cobalt in the amount of 75 % by concentration of the metal, which prevailed the analogues of the control group for this indicator, respectively, by 4.4 kg (р≤0.01), or by 9.7%. Cows of the 2nd, 4th and 5th experimental groups according to the average daily milk fat content of the natural fat were dominated by analogues of control, respectively, by 2.1 kg or 4.8 %; 3.2 kg or 7.1% (p≤0.05); and 1.3 kg, or 2.9 %. During the experiment, fat content of milk of cows of the 3rd experimental group exceeded fat content of milk of control group of cows by 0.03%, and in terms of milk, the 4% fat content milk yield was 3649.7 kg, which is 10.8 % (p≤0.01) higher yields of experimental cows in the control group. As for the content of protein in milk, there was no significant difference between the groups in this indicator. The use of sulfuric acid Cobalt in accordance with the recommended norm in the diet of experimental cows resulted in its positive balance in the body at 4.87 mg per head per day. And in the cows of the 2-5th experimental groups who ate this trace in the form of a mixed ligand complex the balance was, respectively, 8.19 % (p≤0.01), 10.07 (p≤0.01), 8.89 (p≤0.01), and 8.05 (p≤0.01).
Розробка та оцінка придатності методу з визначення антибіотиків у молоці за допомогою рідинної хроматографії високого тиску - тандемної мас-спектрометрії (UPLC-MS-MS)
Authors: Bayer O. V. , Yaremchuk O. S. , Shevchenko L. V. , Mykhalska V. M.
Published: January 2018

On the basis of the conducted research, validation of the method for the determination of sulfonamides and antibiotics in milk by screening method was developed and conducted by LC / MS / MS. It has been confirmed that a method determination by ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC / MS / MS) is more sensitive and selective. Validation of the method was carried out in accordance with the Decision of the European Commission 2002/657 / EC of 12 August 2002, which ensures the implementation of Council Directive 96/23 / EC concerning the effectiveness of analytical methods and the interpretation of results, and in accordance with the recommendations of the reference laboratories of the European Union CRLs of 20/1/2010 on the assessment of the suitability of screening methods for the determination of veterinary drugs. It is proved that the adapted method for the determination of sulfonamides and antibiotics is sensitive and according to parameters meets the European requirements. This method is fast enough, practical and universal, since it allows simultaneous examination of about 70 antibacterial drugs at the same time in milk.

It has been established that the method of determining the residual amount of sulfonamides and antibiotics by the UPLC / MS / MS is suitable for the investigation of milk on the content of the following drugs: sulfaguanidine, sulfacetamide, sulfapiridine, sulfadiazine, sulfamethoxazole, sulfathiazole, sulfamethazine, sulfamethizole, sulfabenzamide, sulfaquinoxaline, sulfadoxine, sulfadimetoxin, sulfanilamide, trimethoprim, sulfamonomethoxine, sulfamethoxypyridazine, sulfachlorpyridazine, sulfamoxol, dapsone, amoxicillin, ampicillin, penicillin G, penicillin V, oxacillin, cloxacillin, nafcillin, dicloxacillin, cefalonium, cefazolin, cefoperazon, cefquinome, cefapirin, ceftiofur, cefalexin, norfloxacin, enrofloxacin, ciprofloxacin, marbofloxacin, difloxacin, danofloxacin, sarafloxacin, flumekvin, oxolinic acid, nalidixic acid, oxytetracycline, chlortetracycline, tetracycline, doxycycline, epi-oxytetracycline, epi-tetracycline, epi-chlortetracycline, streptomycin, dihydrostreptomycin, gentamicin, kanamycin, apramycin, paromomycin, lincomycin, spectinomycin, tilmycosin, erythromycin, josamycin, spiramycin, tylosin, tiamulin, colistin, gamitromycin, tulatromycin. In assessing the suitability of the method, the MS / MS detection parameters are set and the validation characteristics are determined.
Визначення вмісту антибіотиків та сульфаніламідних препаратів у молоці скринінговим методом
Authors: Bayer E. V. , Novozhitskaya Yu. N. , Shevchenko L. V. , Mykhalska V. M.
Published: January 2018

On the basis of the conducted research, an assessment of the suitability of the method for the determination of sulfanilamide preparations and antibiotics in milk by raw screening method was developed and conducted with the help of RX / MS / MS. It has been confirmed that a method based on liquid chromatography using a mass spectrometric detector (PC / MS / MS) is more sensitive and selective. The study was conducted in accordance with the "Plan for monitoring the residues of veterinary drugs and other pollutants in live and unprocessed food of animal origin" approved in Ukraine in accordance with Directive 96/23 / EC dated April 29, 1996. It is proved that the adapted method for the determination of sulfanilamide preparations and antibiotics is sensitive and according to its parameters meets the European requirements. This method is fast enough, practical and universal, since it allows simultaneous examination of about 70 antibacterial drugs at the same time in milk. It has been established that the method of determining the residual amount of sulfanilamide preparations and antibiotics by the PX / MS / MS method is suitable for the investigation of products of animal origin on the content of the following drugs: sulfaguanidine, sulfacetamide, sulfapiridine, sulfadiazinum, sulfamethoxazole, sulfatyazole, sulfamethazine, sulfamethizole, sulfabenzamide, sulfametazine, sulfhinkosolin, sulfadoxine, sulfadimetoxin, sulfanilamide, trimethoprim, sulfamonomethoxin, sulfamethoxypyridazine, sulfachloropyridazine, sulfamoxol, dapsone, amoxicillin, ampicillin, penicillin G, penicillin V, oxacillin, cloxacillin, naftsilin, dicloxacillin, tsefalonium, tsefozolin, cefoperazone, tsefkvinom, tsefopirin, tseftiofur, cephalexin, norfloxacin, enrofloxacin, ciprofloxacin, marbofloksatsyn, dyfloksatsyn, danofloksatsyn, sarafloksatsyn, flumekin, oksolinovuyu acid, nalidixic acid, oxytetracycline, chlortetracycline, tetracycline, doxycycline, epioxytetracycline, epioxytetracycline, epichlortetracycline, streptomycin, dihydrostreptomycin, gentamicin, kanamycin, apramicin, paromomycin, lincomits, Spectinomycin, tilmykozyn, erythromycin, yozamitsyn, spiramycin, tilozyn, tiamulin, colistin, hamitromitsyn, tulatromitsyn. In assessing the suitability of the method, the MS / MS detection parameters are set and the validation characteristics are determined.
Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load
Authors: Gutyj B. , Stybel V. , Darmohray L. , Lavryshyn Y. , Turko I. , Hachak Y. , Shcherbatyy A. , Bushueva I. , Parchenko V. , Kaplaushenko A. , Krushelnytska O.
Published: January 2018

The results of studies of the effect of Cadmium influence on the state of the enzyme and nonenzyme system of antioxidant protection of young cattle organism, namely on the activity of superoxide dismutase, catalase, glutathione peroxidase, level of reduced glutathione, Selenium, vitamins A and E. It was determined that the feeding of young bulls with cadmium chloride in a dose of 0.04 mg / kg of body weight contributed to a decrease both, enzyme and non enzyme level of the system of antioxidant protection (superoxide dismutase by 22%, catalase - by 12%, renewed glutathione by 11%, glutathione peroxidase by 22 %, Selenium - by 18%, vitamin A - by 23%, vitamin E - by 27%,). The lowest level of the antioxidant protection system in the blood of young cattle was set at the sixteenth and twenty-fourth day of the experiment, which is due to the increased activation of lipoperoxidation processes and a disturbance of the balance between the activity of the antioxidant system and the intensity of peroxidation of lipids. The toxic effect of Cadmium contributes to the change in the stationary concentrations of the radical metabolites O2, ˙OH, NO2, which, in their turn, initiate the processes of peroxide oxidation of lipids, indicating an increase in the level of diene conjugates and malonic dialdehyde. The highest level of intermediate and final products of peroxid oxidation of lipids in the blood of young cattle was determined on the thirtieth day of the experiment, while comparing with the control group, they increased by 31 and 27%, respectively.
Changes in essential oil content of Lippia citriodora in response to induction of bio-active compounds and plant growth regulators
Authors: Roodbaraky Mahsa , Mehrafarin Ali , Khalighi-Sigaroodi Farahnaz , Naghdi Badi Hassanali
Published: January 2018

Background & aim: Bioactive compounds and plant growth regulators could change a plant’s essential oil content and composition. In this study, the effect of bioactive compounds and plant growth regulators on lippia citriodora was investigated.
Objective: This experiment was done with three replications on the basis of factorial experiment in randomized complete block design. The bio-regulators were sprayed in four levels, i.e. distilled water, 50 ppm gibberellic acid (GA3) + 50 ppm indole-3-butyric acid (IBA), 50 ppm GA3 + 100 ppm IBA, and 100 ppm GA3 + 50 ppm IBA. The chitosan was sprayed in two levels: distilled water and 400 ppm chitosan. The methanol was sprayed in two levels: distilled water and 5% v/v methanol. The essential oil was extracted by hydrodistillation method and analyzed by GC and GC/MS.
Results: Thirty-two compounds were identified, representing 95.08% of the total essential oil. The main components were E-citral (geranial) (25.02%), Z-citral (neral) (16.04%), ar-curcumene (10.37%), caryophyllene oxide (9.56%), spathulenol (6.83%), limonene (4.99%) and (E)-caryophyllene (2.29%). The studied traits were significantly affected (p < 0.01) by the interaction of bioregulators, methanol and chitosan application.
Conclusion: Foliar application of IBA, GA3, chitosan and methanol could increase aromatic values of L. citriodora essential oil.
Aquatic macroinvertebrates of the Lower Amu Darya
Authors: Palatov D. M. , Rajabov Z. P,
Published: January 2018

Total of 50 species of macroinvertebrates have been found in the Lower Amu Darya River. The most diverse and productive assemblages of macroinvertebrates are formed on flooded woody objects, while the most depleted ones occur on washed sand and clay.
Contamination of Ain Djenane’s water source of the city Tiaret (Algeria) by nitrates, nitrogen and phosphates
Authors: Chafaa M. , Naceur K. , Omar Y. , Maatoug M. , Kharitonov M.
Published: January 2018

Several levies have been made in the groundwater of Ain Djenane of the Tiaret region (Algeria) during the period of April to May 2016 at the level of several wells located in urban areas. These levies have been the subject of analysis according to the techniques of the quality of the water assessment and the recommendations of the World Health Organization. The physical characteristics show that the water is of good quality. Very high nitrate rate has revealed with the chemical analyzes. The physico-chemical analysis has shown that studied wells have toxic substances concentrations below the WHO and national standards. It was indicated a strong contamination with nitrates (98.19±2.46 mg/l), nitrogen (18.0±3.33mg/l), phosphates (0.487±0.06 mg/l), and the contents of suspensions (851.76±18.1 mg/l), respectively. These concentrations are bigger than the standards recommended by the WHO (2006) and the standards of Algeria. The presence very high nitrates content in these waters could constitute a significant health risk to human health.
Application of the photocatalysis for the water purification and forthcoming irrigation
Authors: Omar Y. , Chafaa M. , Bezzerrouk M. A. , Maatoug M. , Hachemi M. F. , Kharitonov M.
Published: January 2018

The considerable development of agricultural and industrial activity, has led to the emergence of new classes of organic pollutants say "persistent" which are resistant to the processes of conventional treatment of the waters. The photocatalysis represents of our days, emerging solution to the problems of pollution of groundwater environments, because that can degrade organic matter in basic products and less toxic. This technique associated with a biological method "bio-monitoring" allows to purify the waters effectively, simple and less costly. The use of photocatalytic process in the presence of photocatalyst SnO2 and Lemna minor under UV light has revealed a decrease in the absorbance by report to the initial state and that the rate of absorption varies from 7% to 33% for wastewater and 7% to 34% for the waters of the dam Dahmouni. The treatment of the waters by photocatalysis associated with the lenses of water could be a reality in the countries with strong sunlight to here to a dozen years. This technique is interesting both from the point of view of technology that from the environmental point of view. In effect, this technique may be extended to large scale for addressing releases industrial or domestic or for the purification of contaminated waters.Two water resourses including the dam Dahmounie and wastewater treatment plant in the city of Tiaret are in in the focus of this study.
Pathogenicity of Simplicillium lanosoniveum to Coccus hesperidum
Authors: Skaptsov M. , Smirnov S. , Kutsev M. , Uvarova O. , Sinitsyna T. , Shmakov A. , Matsyura A.
Published: January 2018

Simplicillium lanosoniveum isolate SSBG2 was isolated from the diseased C. hesper collected from Schefflera octophylla in greenhouse of the South-Siberian Botanical Garden, and identified based on morphological observation and ITS region analysis. The infected plants were inoculated with conidial suspension of the isolate SSBG2 in concentrations 1.0*105/mL. It was showed that after inoculation the C. hesper female adults were more vulnerable to infection. Larvae are affected to a lesser extent. The mycelium grows under the scale and cause the death of the insect. It was indicated that S. lanosoniveum had high infectivity against C. hesper. Infection symptoms appeared on day 7 after the inoculation, the infection reached the peak on day 20. Our study provides a new isolate that affects the Coccus hesperidum.
Применение природного клиноптилолита в кормлении лошадей
Authors: Nekrasov R. V. , Zelenchenkova A. A. , Chabaev M. G. , Tulunay Ç , Shleg E. V.
Published: January 2018

We provided an inclusion of the mineral supplement as clinoptilolite (Nat-Min 9000, fraction 0-1 mm, 70 g per horse/day, Nat-Min 200, fraction 0-0.2 mm, 28 g per head/day) in the ration of sports horses and this was provided a positive effect on the body of experimental animals as evidenced by data of blood biochemistry. The studied blood values were within the physiological norms, the usage of clinoptilolite led to optimization of the biochemical profile of sports horses. The use of the adsorption additive Nat-Min did not adversely affect the concentration of fat-soluble vitamins A and E in the blood of experimental animals. At the end of the experiment, the concentration of vitamins A and E in the blood of the animals of the 2nd test group were above the control by 0.95 - 5.50 μmol/L and by 6.78 - 12.09 μmol/L (p < 0.05) in 3rd test group compared to the control. At the same time, the concentration of vitamin B2 in the horse blood of experimental groups decreased by 0.0037 μmol/ml (p <0.05) in 2nd experimental group and 0,0031 μmol/l in 3rd test group. The content of lysozyme in the blood of animals of the 2nd and 3rd test groups has increased relative to the control level by 0.13 and 0.16 μg/ml, respectively. Phagocytic activity in experimental groups has increased relative to control by 1.40%. A positive effect of the Nat-Min feed additive (fractions of 0-1 and 0-0.2 mm) on the physiological processes in the animals was also established. Variations of mineral additive fodder resulted in a generally equivalent positive biological effect, which makes it possible to recommend the use of clinoptilolite for horse feeding.
Визначення індексу Керногана для судин селезінки пойкілотермних тварин
Authors: Dunaievska O. F.
Published: January 2018

The article presents the results of the determination of the Kernohan index in vessels of the spleen at cold-blooded animals. The index was calculated for the arteries of the white pulp, in particular, the central artery of a lymphoid nodule and lymphoid sheaths near the vessels. This selection is based on the performance of white pulp filtration and immune functions. The study of the spleen conducted in the phase of morphological and functional maturity of the organ in the wells catfish (age 2 years), the lake frogs (age 24-26 months), and the green lizards (aged 23-25 months). The slices of material were fixed in 10-12 % chilled neutral formalin solution, liquid Carnoy, for microscopic studies; then they were embedded in paraffin. Histological sections have a thickness not exceeding 6 micrometers. Then there are dyeing by hematoxylin and eosin according to Van-Gison technology. Morphometric study of the spleen was performed under light microscopy using a microscope Biolam-Lomo and Micros MC-50. The microphotography of histological preparations was carried out with CAM V 200 video camera. It is mounted on a microscope Micros MC-50. The Kernohan index belongs to the indices which characterizing the functional immunomorphology in the spleen and is used to develop test criteria on in biomonitoring.

The wall thickness of the central artery lymphoid nodule is the smallest in the fish (3.4±1.11 micrometers) and the largest in the frog (20.47±4.46 micrometers), of a lizard, it is of 6.96±of 1.62 micrometers. The inner diameter is the smallest in lizards (4.32±2.44 micrometers) and the largest –in the frog (21.03±3.76 micrometers), in the fish is of 6.48±2.31 micrometers. The frog wall thickness of the central artery of lymphoid nodule and arteries lymphoid sheaths near the vessels are the highest (11.25±1.25 micrometers and 31.25±6.25 micrometers, respectively) among the studied animals. The fish`s wall thickness of inner diameter of the artery and arteries lymphoid sheaths near the vessels is 6.25±1.12 micrometers and 16.01±2.55 micrometers; in the lizards – 6.22±1.06 micrometers and was 3.56±0.53 micrometers, respectively. The Kernohan index of arteries lymphoid nodule is the larger than arteries lymphoid sheaths near the vessels by 1.35 times (in fish), by 2.69 times (in frog), and less than by 1.08 times (in lizard). The highest value of Kernohan index was registered for lizard: 3.2±0.61 of artery lymphoid nodule and 3.49±0.59 for arteries lymphoid sheaths near the vessels.
Accumulation of microelements by different invertebrate trophic groups on wasted lands
Authors: Didur O. A. , Kulbachko Yu. L. , Gasso V. Y.
Published: January 2018

The problem of transformation of natural landscapes resulted from the negative technogenic impact is highlighted. It is shown that mining enterprises are powerful anthropo-technical sources of organic and inorganic toxicants entering the environment. Their wastes pollute all components of the ecosystems and negatively influence human health by increasing a risk of disease. The nature of the accumulation of trace elements (Fe, Cu, Zn, Ni, Cd, and Pb) by invertebrate animals of various functional groups under conditions of anthropo-technogenic pressure was studied. The sample plots were located on self-overgrowing sites with ruderal vegetation located in the immediate vicinity of the Mangan ore-dressing and processing enterprise (Dnipropetrovsk region). It is quite naturally that among the studied biogenic microelements (Fe, Cu, Zn and Ni), the phyto-, zoo-, and saprophages in the investigated zone of technogenic pollution most actively accumulate Fe: 22758, 17516 and 18884 mg/kg dry weight on average, respectively. There are significant differences (p ≤ 0.05) in the content of studied microelements between saprophages and phytophages. The saprophages accumulate such trace metals as Mn, Cu, Zn and Cd in high quantities, but Ni and Pb – in smaller ones. The saprophagous functional group of invertebrates is an active agent of detritogenesis, in the conditions of modern nature management it acts as a powerful element of ecosystem engineering (habitat transformation), the main ecological role of which is to modify the habitat of other soil biota. In addition, the saprophages fulfil their concentrating geochemical function. They actively participate in the most important soil biochemical process: the formation of humus, the migration of microelements along trophic chains, the biological cycle in general, and provide such supporting ecosystem services as increasing soil fertility and nutrient cycling.
Прогнозування потреби ремонтного молодняку птиці у гліцині
Authors: Kryvenok M. J. , Ilchuk I. I. , Mykhalska V. M.
Published: January 2018

An effective level of glycine in the diets of replacement chicks has been experimentally established at different periods of their breeding. The influence of different levels of glycine in the mixed fodders of replacement chicks on their growth and development was investigated. It was established that replacement chicks with effective level of glycine in their rations at the end of the growing period had more weight by 167 g than chicks from the control group; this value was nevertheless within the limits recommended by the developer of the cross. We also studied the influence of different glycine levels in mixed fodder of young egg chicks on the digestibility of the nutrients, the balance of nitrogen and amino acid during the whole period of chicks’ growth. It was established that the optimum level of glycine in different growing periods contributed to an increase of protein digestion by 2 percent, fat – 2 percent, fiber – 2 percent, and BER – 1 percent, compared to the control birds. The chicks’ balance of nitrogen was studied and its positive trend was registered for the poultry of all groups. Reducing the amount of glycine in chicken diets as well as its excess leads to a decrease of nitrogen contamination in their bodies. The level of glycine content in chick bodies negatively correlated with its content in the diet. This level was the smallest in animals with excessive levels of glycine in their diet, which in turn led to a decrease in its absorption. Based on the patterns of dietary glycine needs of chickens throughout the entire period of their breeding and its effect on their growth, development, digestibility of nutrients in the body, and balance of nitrogen and investigated amino acids, we calculated the linear regression equation that can be applied in the operational control or in composing of the poultry diet.