bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphology in Dene Languages
Authors: Rice Keren
Published: February 2020

Dene languages are often recognized for their morphological complexity. The languages are known in particular for their complex verbal morphology, and the Dene verb word has received considerable attention in the literature. The verb word is polysynthetic, with several unusual properties: the actual morpheme inventory is relatively small, with rich word formation possibilities; it is prefixing, while suffixing is far more common amongst the world’s languages; what is a single morpheme from a semantic perspective can be discontinuous, illustrating what Whorf calls interrupted synthesis; a single morpheme can be both semantically productive and lexically idiosyncratic; some affixes are mobile; there is considerable homophony; fusion of morphemes is common; and aspects of the phonology lead to surface opacity, with unexpected allomorphy. Several proposals have been introduced to account for the structure of the verb word, and psycholinguistic and sociolinguistic factors that enter in to understanding the complexities of the verb word have been addressed. There is also research on the acquisition of and teaching of the verb word. Overall, it is important to ask what the structure of the verb is synchronically, and what, while interesting, is a consequence of diachronic developments.

In addition to the well-studied complexities of the verb, there are also interesting aspects of other categories. The nouns are worthy of attention for their formation, a noun classification system, and nominal possession. The directional systems of Dene languages tend to be rich, including both a root that indicates direction and a prefix that specifies distance or direction from the speaker. At least some of he languages also have evidentials, and these are, in general, understudied.

Dene languages, like many other languages of North America, are now being learned largely as second languages. This increases the urgency to study areas such as acquisition and language use in order to help in sustaining the languages in communities where this is desired.
Linking Hazard Vulnerability, Risk Reduction, and Adaptation
Authors: Birkmann Jörn , McMillan Joanna M.
Published: February 2020

The concepts of vulnerability, disaster risk reduction and climate change adaptation are interlinked. Risk reduction requires a focus not just on the hazards themselves or on the people and structures exposed to hazards but on the vulnerability of those exposed. Vulnerability helps with the identification of root causes that make people or structures susceptible to being affected by natural and climate-related hazards. It is therefore an essential component of reducing risk of disasters and of adapting to climate change.

The need to better assess and acknowledge vulnerability has been recognized by several communities of thought and practice, including the Disaster Risk Reduction (DRR) and Climate Change Adaptation (CCA) communities. The concept of vulnerability was introduced during the 1980s as a way to better understand the differential consequences of similar hazard events and differential impacts of climate change on different societies or social groups and physical structures. Since then, the concept gradually became an integral part of discourses around disaster risk reduction and climate change adaptation. Although the history of the emergence of vulnerability concepts and the different perspectives of these communities mean the way they frame vulnerability differs, the academic discourse has reached wide agreement that risk—and actual harm and losses—are not just caused by physical events apparently out of human control but primarily by what is exposed and vulnerable to those events.

In the international policy arena, vulnerability, risk, and adaptation concepts are now integrated into the global agenda on sustainable development, disaster risk reduction, and climate change. In the context of international development projects and financial aid, the terms and concepts are increasingly used and applied. However, there is still too little focus on addressing underlying vulnerabilities.
Managing Vulnerability During Cascading Disasters: Language Access Services
Authors: Federici Federico Marco
Published: February 2020

Communication underpins all phases of disaster risk reduction: it is at the heart of risk mitigation, by increasing resilience and preparedness, and by interacting with affected communities in the response phase and throughout the reconstruction and recovery after a disaster. Communication does not alter the scope or severity of a disaster triggered by natural hazards, but the extent to which risk reduction strategies impact on affected regions depends greatly on existing differences inherent in the society of these regions. Ethnic minorities and multilingual language groups―which are not always one and the same―may become vulnerable groups when there has been little or no planning or no awareness of the impact of limited access to trustworthy information when the disaster strikes.

Furthermore, large-scale disasters are likely to involve personnel from the humanitarian sector from both local and international offices. Communication in most large-scale events has progressively become multilingual; from the late 20th and early 21st centuries, it is expected that large disasters see collaboration between intergovernmental, governmental, local, national, and international entities that operate in different ways in rescue and relief operations. Regardless of linguistic contexts, communication of reliable information in a trustworthy manner is complex to achieve in the aftermath of a disaster, which may instantaneously affect telecommunication infrastructures (overloading VOIP and GPS systems). From coordination to information, clear communication plays a role in any activity intending to reduce risks, damages, morbidity, and mortality. Achieving clear communication in crisis management is a feat in a monolingual context: people from different organizations and with different capacities in multi-agency operations have at least a common language, nonetheless, terminology varies from one organization to another, thus hampering successful communication. Achieving effective and clear communication with multilingual communities, while using one language (or lingua franca), such as English, Arabic, Spanish, or Hindi, depending on the region, is impossible without due consideration to language translation.
Agricultural Energy Demand and Use
Authors: Roland-Holst David
Published: February 2020

This overview article examines the historical and technical relationship between agrifood supply chains and energy services. Because agriculture is the original environmental science, all technological change in food production has environmental implications, but these are especially serious in the context of conventional energy use. Agrifood sustainability is of paramount importance to us all, and this will require lower carbon pathways for agriculture.
iMRM: a platform for simultaneously identifying multiple kinds of RNA modifications
Authors: Liu Kewei , Chen Wei
Published: February 2020

Motivation

RNA modifications play critical roles in a series of cellular and developmental processes. Knowledge about the distributions of RNA modifications in the transcriptomes will provide clues to revealing their functions. Since experimental methods are time consuming and laborious for detecting RNA modifications, computational methods have been proposed for this aim in the past five years. However, there are some drawbacks for both experimental and computational methods in simultaneously identifying modifications occurred on different nucleotides.

Results

To address such a challenge, in this article, we developed a new predictor called iMRM, which is able to simultaneously identify m6A, m5C, m1A, ψ and A-to-I modifications in Homo sapiens, Mus musculus and Saccharomyces cerevisiae. In iMRM, the feature selection technique was used to pick out the optimal features. The results from both 10-fold cross-validation and jackknife test demonstrated that the performance of iMRM is superior to existing methods for identifying RNA modifications.

Availability and implementation

A user-friendly web server for iMRM was established at http://www.bioml.cn/XG_iRNA/home. The off-line command-line version is available at https://github.com/liukeweiaway/iMRM.

Contact

greatchen@ncst.edu.cn

Supplementary information

Supplementary data are available at Bioinformatics online.
Isolation of infectious, non-fibrillar and oligomeric prions from a genetic prion disease
Authors: Vanni Ilaria , Pirisinu Laura , Acevedo-Morantes Claudia , Kamali-Jamil Razieh , Rathod Vineet , Di Bari Michele Angelo , D’Agostino Claudia , Marcon Stefano , Esposito Elena , Riccardi Geraldina , Hornemann Simone , Senatore Assunta , Aguzzi Adriano , Agrimi Umberto , Wille Holger , Nonno Romolo
Published: February 2020

Prions are transmissible agents causing lethal neurodegenerative diseases that are composed of aggregates of misfolded cellular prion protein (PrPSc). Despite non-fibrillar oligomers having been proposed as the most infectious prion particles, prions purified from diseased brains usually consist of large and fibrillar PrPSc aggregates, whose protease-resistant core (PrPres) encompasses the whole C-terminus of PrP. In contrast, PrPSc from Gerstmann-Sträussler-Scheinker disease associated with alanine to valine substitution at position 117 (GSS-A117V) is characterized by a small protease-resistant core, which is devoid of the C-terminus. We thus aimed to investigate the role of this unusual PrPSc in terms of infectivity, strain characteristics, and structural features. We found, by titration in bank voles, that the infectivity of GSS-A117V is extremely high (109.3 ID50 U/g) and is resistant to treatment with proteinase K (109.0 ID50 U/g). We then purified the proteinase K-resistant GSS-A117V prions and determined the amount of infectivity and PrPres in the different fractions, alongside the morphological characteristics of purified PrPres aggregates by electron microscopy. Purified pellet fractions from GSS-A117V contained the expected N- and C-terminally cleaved 7 kDa PrPres, although the yield of PrPres was low. We found that this low yield depended on the low density/small size of GSS-A117V PrPres, as it was mainly retained in the last supernatant fraction. All fractions were highly infectious, thus confirming the infectious nature of the 7 kDa PrPres, with infectivity levels that directly correlated with the PrPres amount detected. Finally, electron microscopy analysis of these fractions showed no presence of amyloid fibrils, but only very small and indistinct, non-fibrillar PrPresparticles were detected and confirmed to contain PrP via immunogold labelling. Our study demonstrates that purified aggregates of 7 kDa PrPres, spanning residues ∼90–150, are highly infectious oligomers that encode the biochemical and biological strain features of the original sample. Overall, the autocatalytic behaviour of the prion oligomers reveals their role in the propagation of neurodegeneration in patients with Gerstmann-Sträussler-Scheinker disease and implies that the C-terminus of PrPSc is dispensable for infectivity and strain features for this prion strain, uncovering the central PrP domain as the minimal molecular component able to encode infectious prions. These findings are consistent with the hypothesis that non-fibrillar prion particles are highly efficient propagators of disease and provide new molecular and morphological constraints on the structure of infectious prions.
Age-Related Decline in the Topological Efficiency of the Brain Structural Connectome and Cognitive Aging
Authors: Li Xin , Wang Yezhou , Wang Wenxiao , Huang Weijie , Chen Kewei , Xu Kai , Zhang Junying , Chen Yaojing , Li He , Wei Dongfeng , Shu Ni , Zhang Zhanjun
Published: February 2020

Brain disconnection model has been proposed as a possible neural mechanism for cognitive aging. However, the relationship between structural connectivity degeneration and cognitive decline with normal aging remains unclear. In the present study, using diffusion MRI and tractography techniques, we report graph theory-based analyses of the brain structural connectome in a cross-sectional, community-based cohort of 633 cognitively healthy elderly individuals. Comprehensive neuropsychological assessment of the elderly subjects was performed. The association between age, brain structural connectome, and cognition across elderly individuals was examined. We found that the topological efficiency, modularity, and hub integration of the brain structural connectome exhibited a significant decline with normal aging, especially in the frontal, parietal, and superior temporal regions. Importantly, network efficiency was positively correlated with attention and executive function in elderly subjects and had a significant mediation effect on the age-related decline in these cognitive functions. Moreover, nodal efficiency of the brain structural connectome showed good performance for the prediction of attention and executive function in elderly individuals. Together, our findings revealed topological alterations of the brain structural connectome with normal aging, which provides possible structural substrates underlying cognitive aging and sensitive imaging markers for the individual prediction of cognitive functions in elderly subjects.
The Effects of Children's Gender Composition on Filial Piety and Old-Age Support*
Authors: Guo Rufei , Zhang Junsen
Published: February 2020

Do parents forge children’s preference for old-age support? Becker (1993) conjectures that the inculcation of filial piety increases parents’ investment in children’s human capital. We provide the first empirical evidence on parents’ instilling of filial piety in children, by combining the natural experiment of twins with China’s One-Child Policy to obtain exogenous variations in children’s gender composition. Among the different models of filial-piety inculcation, our empirical results favour a Beckerian model of altruism inculcation in which parents solicit support from the child with a higher earnings endowment.
Partial lung resection by uniportal video-assisted thoracoscopic surgery: technique and pitfalls
Authors: Hirai Kyoji , Usuda Jitsuo
Published: February 2020

The use of uniportal video-assisted thoracoscopic surgery (VATS) has increased worldwide. The number of facilities introducing this simple and cost-effective surgical procedure in Japan has also increased. Partial lung resection is performed to diagnose or treat various cases and surgeons are required to flexibly deal with it. This report describes the technique and pitfalls of partial lung resection by uniportal VATS.
Altered aortic arch geometry in patients with type B aortic dissection
Authors: Cao Long , Lu Weihang , Ge Yangyang , Wang Xinhao , He Yuan , Sun Guoyi , Liu Jie , Liu Xiaoping , Jia Xin , Xiong Jiang , Ma Xiaohui , Zhang Hongpeng , Wang Lijun , Guo Wei
Published: February 2020

OBJECTIVES

This study aims to evaluate differences in proximal aorta geometry and identify specific anatomical predictors of type B aortic dissection (TBAD).

METHODS

We evaluated computed tomographic angiograms of controls (n = 185) and patients with acute TBAD (n = 173). Using propensity score matching, we created 2 groups of 127 patients. 3mensio Vascular software was used to analyse the computed tomographic angiograms and measure the diameter, length, tortuosity index and angulation of the proximal aorta (divided into ascending aorta and aortic arch). Tortuosity index was calculated by dividing the centre lumen line length of the aortic segment by its shortest length. Angulation was measured by the centre lumen line ‘tangent line angle’. Two independent multivariable models identified significant anatomical associations regarding the tortuosity and angulation geometry.

RESULTS

Aortic diameter and ascending aorta and aortic arch lengths in TBAD increased significantly. The aortic arch tortuosity was significantly higher in the TBAD group (P < 0.001), with no difference regarding the ascending aorta (P = 0.11). Ascending aorta and aortic arch angulation were significantly higher in the TBAD group (P = 0.01, P < 0.001, respectively). Multivariable analyses showed that increased aortic arch tortuosity and angulation were significant predictors of the development of TBAD [odds ratio (OR) 1.91, 95% confidence interval (CI) 1.40–2.59; P < 0.001 and OR 1.08, 95% CI 1.04–1.12; P < 0.001], respectively.

CONCLUSIONS

In addition to proximal aorta dilation and elongation, we identified increased aortic arch tortuosity and angulation as possible specific predictors of TBAD.
Optimal versus suboptimal mitral valve repair: late results in a matched cohort study
Authors: De Bonis Michele , Zancanaro Edoardo , Lapenna Elisabetta , Trumello Cinzia , Ascione Guido , Giambuzzi Ilaria , Ruggeri Stefania , Meneghin Roberta , Abboud Sabrin , Agricola Eustachio , Del Forno Benedetto , Buzzatti Nicola , Monaco Fabrizio , Pappalardo Federico , Castiglioni Alessandro , Alfieri Ottavio
Published: February 2020

OBJECTIVES

After mitral repair for degenerative mitral regurgitation (MR), no or mild (≤1+/4+) residual MR should remain. Occasionally patients are left with more than mild residual MR (>1+/4+) for a number of reasons. The aim of this study was to assess the late implications of such a suboptimal repair in a matched cohort study.

METHODS

From 2006 to 2013, a total of 2158 patients underwent mitral repair for degenerative MR in our institution. Fifty patients (2.3%) with residual MR >1+ at hospital discharge (study group) were matched up to 1:2 with 91 patients operated on during the same period who were discharged with MR ≤1+ (control group). The median follow-up was 8 years (interquartile range 6.3–10.1, longest 12.7 years). A comparative analysis of the outcomes in the 2 groups was performed.

RESULTS

Overall survival at 8 years was 87 ± 8% in the study group and 92 ± 3% in the control group (P = 0.23). There were 3 late deaths (6.0%) in the study group and 6 deaths (6.6%) in the control group. Freedom from reoperation was similar (P = 1.0). At 8 years the prevalence of MR ≥3+ was significantly higher in the study group (15.6% vs 2.1%, P < 0.001) as was the use of diuretics, beta-blockers and angiotensin-converting enzyme inhibitors/angiotensin-receptor blockers. Interestingly, even in the control group, a gradual progression of MR was observed because 13.3% of the patients had MR ≥2+ at 8 years with a significant increase over time (P < 0.001).

CONCLUSIONS

Residual MR more than mild at hospital discharge is associated with lower durability of mitral repair and the need for more medical therapy in the long term. However, even an initial optimal result does not completely arrest the progression of the degenerative process.
Physical Activity as Moderator of the Association Between APOE and Cognitive Decline in Older Adults: Results from Three Longitudinal Cohort Studies
Authors: Stringa Najada , van Schoor Natasja M , Milaneschi Yuri , Ikram M Arfan , Del Panta Vieri , Koolhaas Chantal M , Voortman Trudy , Bandinelli Stefania , Wolters Frank J , Huisman Martijn
Published: February 2020

Background

Previous studies have suggested that the association between APOE ɛ 4 and dementia is moderated by physical activity (PA), but the results remain inconclusive and longitudinal data on cognitive decline are missing. In this study, we examine whether there is a gene–environment interaction between APOE and PA on cognitive decline in older adults using 9-year follow-up data of three cohort studies.

Methods

We followed 7,176 participants from three longitudinal cohort studies: Longitudinal Aging Study Amsterdam (LASA), InCHIANTI, and Rotterdam Study for 9 years. PA was assessed with self-reported questionnaires and was categorized in low, moderate, and high PA. Cognitive function was assessed with the Mini-Mental State Examination (MMSE) and cognitive decline was defined as a decrease of three points or more on the MMSE during 3 years follow-up. We fitted logistic regression models using generalized estimating equations adjusting for age, sex, education, depressive symptoms, and number of chronic disease. Interaction between APOE and PA was tested on multiplicative and additive scale.

Results

Cohorts were similar in most aspects but InCHIANTI participants were on average older and had lower education. APOE ɛ 4 carriers had higher odds of cognitive decline (odds ratio [OR] = 1.46, 95% confidence interval [CI]: 1.29–1.64) while PA was not significantly associated with cognitive decline overall (moderate PA: OR = 0.87, 0.67–1.13; high PA: OR = 0.71, 0.36–1.40). There was no evidence for an interaction effect between PA and APOE ɛ 4 in cognitive decline in older adults (APOE × moderate PA: p = .83; APOE × high PA: p = .90).

Conclusions

Previous claims of a gene–environment interaction between APOE ɛ 4 and PA in cognitive decline are not supported by our results.
Does Winning a Patent Race lead to more follow-on Innovation?
Authors: Thompson Neil C , Kuhn Jeffrey M
Published: February 2020

Competition between firms to invent and patent an idea, or “patent racing,” has been much discussed in theory, but seldom analyzed empirically and never at scale. This article introduces an empirical way to identify patent races, and provides the first broad-based view of them in the real world. It reveals that patent races are common, particularly in information-technology fields. The article then analyzes the effect of winning a patent race, showing that patent race winners do significantly more follow-on innovation, and their follow-on research is more similar to what was covered by the patent.

(JEL CODES: O34, O32, O31)
New candidates for regulated gene integrity revealed through precise mapping of integrative genetic elements
Authors: Mageeney Catherine M , Lau Britney Y , Wagner Julian M , Hudson Corey M , Schoeniger Joseph S , Krishnakumar Raga , Williams Kelly P
Published: February 2020

Integrative genetic elements (IGEs) are mobile multigene DNA units that integrate into and excise from host bacterial genomes. Each IGE usually targets a specific site within a conserved host gene, integrating in a manner that preserves target gene function. However, a small number of bacterial genes are known to be inactivated upon IGE integration and reactivated upon excision, regulating phenotypes of virulence, mutation rate, and terminal differentiation in multicellular bacteria. The list of regulated gene integrity (RGI) cases has been slow-growing because IGEs have been challenging to precisely and comprehensively locate in genomes. We present software (TIGER) that maps IGEs with unprecedented precision and without attB site bias. TIGER uses a comparative genomic, ping-pong BLAST approach, based on the principle that the IGE integration module (i.e. its int-attP region) is cohesive. The resultant IGEs from 2168 genomes, along with integrase phylogenetic analysis and gene inactivation tests, revealed 19 new cases of genes whose integrity is regulated by IGEs (including dut, eccCa1, gntT, hrpB, merA, ompN, prkA, tqsA, traG, yifB, yfaT and ynfE), as well as recovering previously known cases (in sigK, spsM, comK, mlrA and hlb genes). It also recovered known clades of site-promiscuous integrases and identified possible new ones.
Association of motivations and barriers with participation and performance in a pedometer-based intervention
Authors: Sheshadri Anoop , Kittiskulnam Piyawan , Delgado Cynthia , Sudore Rebecca , Lai Jennifer C , Johansen Kirsten L
Published: February 2020

Background

A randomized trial of a pedometer-based intervention with weekly activity goals led to increased walking among dialysis patients. However, the association of participant-expressed motivations and barriers to participation and performance in such an intervention has not been determined.

Methods

Thirty dialysis patients were randomized to a 12-week pedometer-based intervention with weekly step goals. Participants were asked about motivations and barriers to the increasing activity via weekly semi-scripted telephone interviews. We examined the association of these motivations and barriers with achieving weekly goals, reaching overall targets and increasing steps through multivariable linear and logistic regression analyses adjusted for age, sex, body mass index, dialysis modality and baseline steps.

Results

The most common motivations were desire to maintain/improve functional ability (30%) and activity (30%). The most common barriers were health-related (33%). Motivation to maintain/improve functional ability was associated with achieving weekly goals 17.9% more often [95% confidence interval (CI) 1.7–34.2] and with a greater increase in steps (1524 steps; 95% CI 61–2989) than those lacking this motivation. Experiencing a health-related barrier was not associated with the decreased achievement of weekly goals but was associated with lower odds of reaching overall targets (odds ratio = 0.06; 95% CI 0.01–0.53) and a smaller increase in steps (−1640 steps, 95% CI −3244 to −36). No patients who reported weather/environmental barriers or safety concerns reached overall targets.

Conclusions

Participants who express a desire to maintain/improve functional ability may be particularly suited for activity interventions. Health-related setbacks should be met with revised goals. Reporting environmental or safety concerns may merit lowering overall targets.
Treatment of an aggressive orthotopic murine glioblastoma model with combination checkpoint blockade and a multivalent neoantigen vaccine
Authors: Liu Connor J , Schaettler Maximilian , Blaha Dylan T , Bowman-Kirigin Jay A , Kobayashi Dale K , Livingstone Alexandra J , Bender Diane , Miller Christopher A , Kranz David M , Johanns Tanner M , Dunn Gavin P
Published: February 2020

Background

Although clinical trials testing immunotherapies in glioblastoma (GBM) have yielded mixed results, new strategies targeting tumor-specific somatic coding mutations, termed “neoantigens,” represent promising therapeutic approaches. We characterized the microenvironment and neoantigen landscape of the aggressive CT2A GBM model in order to develop a platform to test combination checkpoint blockade and neoantigen vaccination.

Methods

Flow cytometric analysis was performed on intracranial CT2A and GL261 tumor-infiltrating lymphocytes (TILs). Whole-exome DNA and RNA sequencing of the CT2A murine GBM was employed to identify expressed, somatic mutations. Predicted neoantigens were identified using the pVAC-seq software suite, and top-ranking candidates were screened for reactivity by interferon-gamma enzyme linked immunospot assays. Survival analysis was performed comparing neoantigen vaccination, anti-programmed cell death ligand 1 (αPD-L1), or combination therapy.

Results

Compared with the GL261 model, CT2A exhibited immunologic features consistent with human GBM including reduced αPD-L1 sensitivity and hypofunctional TILs. Of the 29 CT2A neoantigens screened, we identified neoantigen-specific CD8+ T-cell responses in the intracranial TIL and draining lymph nodes to two H2-Kb restricted (Epb4H471L and Pomgnt1R497L) and one H2-Db restricted neoantigen (Plin2G332R). Survival analysis showed that therapeutic neoantigen vaccination with Epb4H471L, Pomgnt1R497L, and Plin2G332R, in combination with αPD-L1 treatment was superior to αPD-L1 alone.

Conclusions

We identified endogenous neoantigen specific CD8+ T cells within an αPD-L1 resistant murine GBM and show that neoantigen vaccination significantly augments survival benefit in combination with αPD-L1 treatment. These observations provide important preclinical correlates for GBM immunotherapy trials and support further investigation into the effects of multimodal immunotherapeutic interventions on antiglioma immunity.

Key Points

1. Neoantigen vaccines combined with checkpoint blockade may be promising treatments.

2. CT2A tumors exhibit features of human GBM microenvironments.

3. Differential scanning fluorimetry assays may complement in silico neoantigen prediction tools.
Simulating the Impact of a Cigarette Minimum Floor Price Law on Adult Smoking Prevalence in California
Authors: Golden Shelley D , Kim KyungSu , Kong Amanda Y , Tao Vivian Qingzi , Carr Derek , Musburger Pratima
Published: February 2020

Introduction

Minimum floor price laws (MFPLs) are an emerging tobacco control policy that sets a minimum price below which a specific tobacco product cannot be sold. MFPLs target cheaper products and may disproportionately impact consumers choosing low price brands or using discounts to reduce prices. We developed a static microsimulation model for California, United States to project short-term effects of different MFPL options for a 20-stick pack of cigarettes on adult smoking behaviors.

Aims and Methods

We simulated 300 000 individuals defined by race and ethnicity, sex, age, and poverty status. Smoking behaviors and cigarette prices were assigned based on demographic distributions in the 2014–2016 California Behavioral Risk Factor Surveillance System. We drew 100 random samples (n = 30 000), weighted to state-level California demographic characteristics. We simulated six MFPL options and modeled impacts on smoking prevalence and cigarette consumption, in general, and separately for those in households below or above 250% of the federal poverty level, assuming a price elasticity of −0.4.

Results

Predicted changes in prices, prevalence, and consumption increased exponentially as the floor price increased from $7.00 to $9.50. Assuming 15% policy avoidance, projected increases in average cigarette prices ranged from $0.19 to $1.61. Decreases in smoking prevalence ranged from 0.05 to 0.43 percentage points, and decreases in average monthly cigarette consumption ranged from 1.4 to 12.3 cigarettes. Projected prices increased, and prevalence and consumption decreased, more among individuals in households below 250% federal poverty level.

Conclusions

MFPLs are a promising tobacco control strategy with the potential to reduce socioeconomic disparities in cigarette smoking prevalence and consumption.

Implications

Despite reductions in adult smoking prevalence, significant socioeconomic disparities remain, with lower-income groups smoking at substantially higher levels than higher-income groups. Policies that set a floor price below which a tobacco product cannot be sold could reduce socioeconomic disparities in smoking, depending on variation in prices paid by smokers prepolicy. By using a microsimulation model to predict changes in smoking for different population groups in California under several floor price scenarios, this study demonstrates that MFPLs have the potential to reduce adult smoking prevalence overall, and especially for lower-income tobacco users.
Functional feeding response of Nordic and Arctic krill on natural phytoplankton and zooplankton
Authors: Cabrol Jory , Fabre Anaïs , Nozais Christian , Tremblay Réjean , Starr Michel , Plourde Stéphane , Winkler Gesche
Published: February 2020

Krill species play a pivotal role in energetic transfer from lower to upper trophic levels. However, functional feeding responses, which determine how food availability influences ingestion rates, are still not well defined for northern krill species. Here, we estimated and compared the functional feeding responses on natural communities of phytoplankton and mesozooplankton of two coexisting species, Meganyctiphanes norvegica and Thysanoessa raschii. We tested the influence of the presence of phytoplankton on the ingestion rate and the selectivity of both krill species when feeding on zooplankton prey. We performed a series of feeding experiments using increasing concentrations of natural phytoplankton (64 taxa; 2 to >50 μm) and mesozooplankton (28 taxa; ~100–2000 μm) assemblages and the latter in presence and absence of phytoplankton. Results revealed that both krill species exhibited a Holling type III feeding response on phytoplankton. However, T. raschii was able to exploit efficiently the highest phytoplankton concentrations. Our experiments highlighted that the presence of phytoplankton modified the functional feeding response on mesozooplankton preys of M. norvegica, but not that of T. raschii. Similarly, the presence of phytoplankton influenced the feeding selectivity on mesozooplankton preys, although both species showed contrasting selectivity patterns. In addition, we estimated the energy needs in relation to the daily rations. T. raschii satisfied its energy needs by feeding either on high phytoplankton concentrations or on low mesozooplankton densities, whereas M. norvegica did not cover its metabolic costs efficiently by feeding on phytoplankton only, even at high phytoplankton concentrations.
Tournament Incentives and Acquisition Performance
Authors: Hasan Iftekhar , Navone Marco , To Thomas Y , Wu Eliza
Published: February 2020

This paper examines the impact of promotion-based tournament incentives on corporate acquisition performance. Measuring tournament incentives as the compensation ratio between the CEO and other senior executives, we show that acquirers with greater tournament incentives experience lower announcement returns. Further analysis shows that the negative effect is driven by the risk-seeking behavior of senior executives induced by tournament incentives. Our results are robust to alternative identification strategies. Our evidence highlights that senior executives, in addition to the CEO, play an influential role in acquisition decisions. (JEL G30, G34, G41, J31, J33, J62)

Received: November 5, 2018; editorial decision January 6, 2020 by Editor Isil Erel.
Exogenous melatonin alleviates cadmium uptake and toxicity in apple rootstocks
Authors: He Jiali , Zhuang Xiaolei , Zhou Jiangtao , Sun Luyang , Wan Huixue , Li Huifeng , Lyu Deguo
Published: February 2020

To examine the potential roles of melatonin in cadmium (Cd) uptake, accumulation and detoxification in Malus plants, we exposed two different apple rootstocks varying greatly in Cd uptake and accumulation to either 0 or 30 μM Cd together with 0 or 100 μM melatonin. Cadmium stress stimulated endogenous melatonin production to a greater extent in the Cd-tolerant Malus baccata Borkh. than in the Cd-susceptible Malus micromalus ‘qingzhoulinqin’. Melatonin application attenuated Cd-induced reductions in growth, photosynthesis and enzyme activity, as well as reactive oxygen species (ROS) and malondialdehyde accumulation. Melatonin treatment more effectively restored photosynthesis, photosynthetic pigments and biomass in Cd-challenged M. micromalus ‘qingzhoulinqin’ than in Cd-stressed M. baccata. Exogenous melatonin lowered root Cd2+ uptake, reduced leaf Cd accumulation, decreased Cd translocation factors and increased root, stem and leaf melatonin contents in both Cd-exposed rootstocks. Melatonin application increased both antioxidant concentrations and enzyme activities to scavenge Cd-induced ROS. Exogenous melatonin treatment altered the mRNA levels of several genes regulating Cd uptake, transport and detoxification including HA7, NRAMP1, NRAMP3, HMA4, PCR2, NAS1, MT2, ABCC1 and MHX. Taken together, these results suggest that exogenous melatonin reduced aerial parts Cd accumulation and mitigated Cd toxicity in Malus plants, probably due to the melatonin-mediated Cd allocation in tissues, and induction of antioxidant defense system and transcriptionally regulated key genes involved in detoxification.
How do international gastric cancer prevention guidelines influence clinical practice globally?
Authors: Gasenko Evita , Leja Marcis , Polaka Inese , Hegmane Alinta , Murillo Raul , Bordin Dmitry , Link Alexander , Kulju Minna , Mochalski Pawel , Shani Gidi , Malfertheiner Peter , Herrero Rolando , Haick Hossam
Published: February 2020

Clinical guidelines recommend particular approaches, including ‘screen-and-treat’ strategy for Helicobacter pylori, to prevent gastric cancer. However, little of this is implemented in clinical practice. The aim of the study was to identify barriers to implementation of international guidelines. A web-based questionnaire distributed globally to specialists in the field. Altogether 886 responses from 75 countries were received. Of the responders, 570 (64%) were men of mean age 47 years. There were 606 gastroenterologists and 65 epidemiologists among the responders. Altogether, 79.8% of the responders disagreed that the burden of gastric cancer is a diminishing problem. ‘Screen-and-treat’ strategy for H. pylori in the responder’s country was considered appropriate by 44.4%, inappropriate by 24.3%, with 31.3% being uncertain. Population-based screening for gastric cancer was considered appropriate in the respective home-country by 62.2%, in other areas – but not the home country – by 27.6%, and inappropriate by 10.2%. As a screening tool, upper endoscopy was acceptable by 35.6%, upper X-ray series by 55.3%, pepsinogens by 26.2% and breath-tests by 23.4%; accuracy, cost-effectiveness and feasibility among the tests varied widely. The attitude towards H. pylori vaccination was that 4.6% of the responders were eager to start vaccination immediately, 55.9% were supporting vaccination but considered that more data are required 12% were negative, and 27.6% did not have an opinion. In general, the attitude of the specialists was in line with guidelines, but was not always translated into clinical practice, particularly in the case of ‘screen-and-treat’ strategy.
Severity of Traumatic Brain Injury in Older Adults and Risk of Ischemic Stroke and Depression
Authors: Vadlamani Aparna , Albrecht Jennifer S.
Published: February 2020

Objective:

Risk of ischemic stroke and depression is elevated among older adults following traumatic brain injury (TBI), yet little is known about how the severity of TBI influences risk. Thus, our objective was to assess the association between severity of the index TBI and risk of ischemic stroke and depression in a sample of older adults treated for TBI.

Design:

Retrospective cohort study.

Setting:

R Adams Cowley Shock Trauma Center.

Participants:

Adults 65 years and older treated for TBI between 2006 and 2010 who survived to hospital discharge and could be linked to their Medicare administrative claims data with continuous enrollment for at least 6 months pre-TBI and 12 months post-TBI.

Main Measures:

First dates of ischemic stroke and depression available in Medicare claims were used to exclude individuals with a history. Next, we separately assessed the association between TBI severity and time to first stroke and depression using Cox proportional hazards models.

Results:

Among 132 patients without preexisting history of stroke, high TBI severity was associated with increased risk of stroke compared with low TBI severity (adjusted hazard ratio 6.68, 95% confidence interval 2.49-17.94). Among 163 patients without preexisting history of depression, high TBI severity was not significantly associated with increased risk of depression compared with low TBI severity (adjusted hazard ratio 1.90, 95% confidence interval 0.94-3.84).

Conclusion:

In this group of older adults with TBI, higher TBI severity was associated with increased risk of ischemic stroke, but not depression. These results suggest that increased monitoring of older adults with moderate-severe TBI for stroke may be warranted.
Sleep Disturbances Following Traumatic Brain Injury in Older Adults: A Comparison Study
Authors: Wei Li , Wen Ya-Ting , Thompson Hilaire J. , Liu Cyong-Yi , Su Yu-Kai , Chen Pin-Yuan , Chen Ching-Yi , Chuang Yeu-Hui , Lin Ya-Jui , Chen Chun-Ting , Chen Ching-Chang , Chiu Hsiao-Ting , Chiu Hsiao-Yean
Published: February 2020

Objectives:

To compare the prevalence of sleep disturbances in older adults with traumatic brain injury (TBI) with that of age- and gender-matched controls and to determine the risk factors for post-TBI sleep disturbances and the effects of post-TBI disturbances on quality of life (QOL).

Design:

Cross-sectional case-comparison study.

Participants:

Eighty older adults (aged ≥65 years) with first-time TBI more than 3 months since injury and 80 older adults controls without TBI who completed sleep and health-related QOL questionnaires.

Results:

Older adults with TBI showed a higher prevalence of obstructive sleep apnea (OSA), insomnia, and daytime sleepiness than older adult controls. Being male, having higher levels of depression and pain, and the presence of insomnia were significantly correlated with the risks of OSA, insomnia, and daytime sleepiness following TBI, respectively. Both OSA and insomnia were significantly correlated with low QOL in older adults with TBI.

Conclusions:

Sleep disturbances are highly prevalent in older adults with TBI. Gender differences, depression severity, and pain level are correlated with the occurrence of post-TBI sleep disturbances. Both OSA and insomnia are regarded as major contributors to low QOL in older people with TBI. Interventions targeted at post-TBI sleep disturbances may improve QOL of older adults.
Evidence-Based Care of Children With Tracheostomies: Hospitalization to Home Care
Authors: Lawrence Patricia R. , Chambers Rebecca , Faulkner Melissa Spezia , Spratling Regena
Published: February 2020

Routine tracheostomy care in children maintains airway patency, minimizes infection, and ensures skin integrity around the tracheostomy stoma to prevent complications. Using evidence-based recommendations for care of the mature tracheostomy limits variation in practice and leads to better patient outcomes in all care settings. Incorporating evidence-based care into practice is especially important because children with tracheostomies are at high risk for morbidity and mortality. The purpose of this review is to summarize the most current, evidence-based literature for pediatric tracheostomy care, including stoma care and tracheostomy suctioning. Rehabilitation nurses can then include these best practices when caring for children with tracheostomies and when educating caregivers who provide tracheostomy care to children at home.
The Association Between ABO Blood Type and Mortality Among Severely Injured Trauma Patients
Authors: Griffin Russell L. , Jansen Jan O. , Bosarge Patrick L. , Marques Marisa B. , Kerby Jeffrey D.
Published: February 2020

Background:

Prior research has reported an association among trauma patients between blood type O and adverse events. More recently, another study reported that severely injured trauma patients of mostly O Rh positive blood type were more likely to die.

Objective:

The objective of the current study is to examine whether the same increased association is observed for blood type O severely injured patients in a more generalizable population comprised of Rh positive and Rh negative individuals.

Methods:

Patients admitted to a Level-I academic trauma center between 2015 and 2018 with severe injury (Injury Severity Score >15) were included in this retrospective cohort study. Logistic regression estimated odds ratios (ORs) and 95% confidence intervals (CIs) for the association between blood type and mortality.

Results:

Among 3,913 patients, a majority were either blood type O (47.5%) or A (34.7%) and 60% were Rh positive. There was no observed difference in complication rates by blood type, and there was no observed significant association with death overall or by cause of death. There were weak, increased associations for blood type B (OR 1.61, 95% CI 0.74–3.53) and type O (OR 1.57, 95% CI 0.90–2.76) compared with blood type A patients.

Conclusion:

Contrary to prior research, the current results suggest no association between blood type and mortality among severely injured trauma patients.
A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2
Authors: Zhao Zhen , Cui Haodong , Song Wenxing , Ru Xiaoling , Zhou Wenhua , Yu Xuefeng
Published: February 2020

1

The ongoing outbreak of the novel coronavirus disease 2019 (COVID-19) originating from Wuhan, China, draws worldwide concerns due to its long incubation period and strong infectivity. Although RT-PCR-based molecular diagnosis techniques are being widely applied for clinical diagnosis currently, timely and accurate diagnosis are still limited due to labour intensive and time-consuming operations of these techniques. To address the issue, herein we report the synthesis of poly (amino ester) with carboxyl groups (PC)-coated magnetic nanoparticles (pcMNPs), and the development of pcMNPs-based viral RNA extraction method for the sensitive detection of COVID-19 causing virus, the SARS-CoV-2. This method combines the lysis and binding steps into one step, and the pcMNPs-RNA complexes can be directly introduced into subsequent RT-PCR reactions. The simplified process can purify viral RNA from multiple samples within 20 min using a simple manual method or an automated high-throughput approach. By identifying two different regions (ORFlabandNgene) of viral RNA, a 10-copy sensitivity and a strong linear correlation between 10 and 105copies of SARS-CoV-2 pseudovirus particles are achieved. Benefitting from the simplicity and excellent performances, this new extraction method can dramatically reduce the turn-around time and operational requirements in current molecular diagnosis of COVID-19, in particular for the early clinical diagnosis.
Temporal dynamics of visual representations in the infant brain
Authors: Bayet Laurie , Zinszer Benjamin D. , Reilly Emily , Cataldo Julia K. , Pruitt Zoe , Cichy Radoslaw M. , Nelson Charles A. , Aslin Richard N.
Published: February 2020

Tools from computational neuroscience have facilitated the investigation of the neural correlates of mental representations. However, access to the representational content of neural activations early in life has remained limited. We asked whether patterns of neural activity elicited by complex visual stimuli (animals, human body) could be decoded from EEG data gathered from 12-15-month-old infants and adult controls. We assessed pairwise classification accuracy at each time-point after stimulus onset, for individual infants and adults. Classification accuracies rose above chance in both groups, within 500 ms. In contrast to adults, neural representations in infants were not linearly separable across visual domains. Representations were similar within, but not across, age groups. These findings suggest a developmental reorganization of visual representations between the second year of life and adulthood and provide a promising proof-of-concept for the feasibility of decoding EEG data within-subject to assess how the infant brain dynamically represents visual objects.
A plasmid locus associated withKlebsiellaclinical infections encodes a microbiome-dependent gut fitness factor
Authors: Vornhagen Jay , Bassis Christine M. , Ramakrishnan Srividya , Hein Robert , Mason Sophia , Bergman Yehudit , Sunshine Nicole , Fan Yunfan , Timp Winston , Schatz Michael C. , Young Vincent B. , Simner Patricia J. , Bachman Michael A.
Published: February 2020

Introductory paragraph

Gut colonization by the pathogenKlebsiella pneumoniae(Kp) is consistently associated with subsequent Kp disease1-5, and patients are predominantly infected with their colonizing strain1,2. However, colonizing strains likely vary in their potential to cause infection. We previously identified the plasmid-encoded tellurium resistance (ter) operon as highly associated with infection when compared to asymptomatic colonization in hospitalized patients1. Theteroperon bestows resistance to the toxic compound tellurite oxide (TeO3−2), but this is unlikely to be its physiological function, as tellurium and TeO3−2are exceedingly rare. Here we show thatterCis necessary andterZABCDEFis sufficient for phenotypic TeO3−2resistance. Next, we demonstrate thatteris encoded on a diverse group of plasmids without known plasmid-encoded virulence genes, suggesting an independent role in infection. Finally, our studies indicate thatteris a gut fitness factor, and its fitness advantage is conferred only when specific gut microbiota constituents are present. Collectively, these data reveal the Kpteroperon that is highly associated with human infection likely acts early in pathogenesis as a horizontally-transferrable fitness factor promoting robust gut colonization in the presence of the indigenous microbiota.