bluuebunny/crossref_metadata_2025 huggingface dataset:
The Detection of Kiwifruit Sunscald Using Spectral Reflectance Data Combined with Machine Learning and CNNs
Authors: Wu Ke , Jia Zhicheng , Duan Qifeng
Published: August 2023

Sunscald in kiwifruit, an environmental stress caused by solar radiation during the summer, reduces fruit quality and yields and causes economic losses. The efficient and timely detection of sunscald and similar diseases is a challenging task but helps to implement measures to control stress. This study provides high-precision detection models and relevant spectral information on kiwifruit physiology for similar statuses, including early-stage sunscald, late-stage sunscald, anthracnose, and healthy. Primarily, in the laboratory, 429 groups of spectral reflectance data for leaves of four statuses were collected and analyzed using a hyperspectral reflection acquisition system. Then, multiple modeling approaches, including combined preprocessing methods, feature extraction algorithms, and classification algorithms, were designed to extract bands and evaluate the performance of the models to detect the statuses of kiwifruit. Finally, the detection of different stages of kiwifruit sunscald under anthracnose interference was accomplished. As influential bands, 694–713 nm, 758–777 nm, 780–799 nm, and 1303–1322 nm were extracted. The overall accuracy, precision, recall, and F1-score values of the models reached 100%, demonstrating an ability to detect all statuses with 100% accuracy. It was concluded that the combined processing of moving average and standard normal variable transformations (MS) could significantly improve the data; the near-infrared support vector machine and visible convolutional neural network with MS (NIR-MS-SVM and VIS-MS-CNN) were established as high-precision detection techniques for the classification of similar kiwifruit statuses, demonstrating 25.58% higher accuracy than the single support vector machine. The VIS-MS-CNN model reached convergence with a stable cross-entropy loss of 0.75 in training and 0.77 in validation. The techniques developed in this study will improve orchard management efficiency and yields and increase researchers’ understanding of kiwifruit physiology.
Cytological Quantification of Nodal Mast Cells in Dogs Affected by Non-Neoplastic Condition and Mast Cell Tumor Using Different Sample Preparation Techniques: An Explorative Study
Authors: Buzzi Giulia , Gambini Matteo , Recordati Camilla , Grieco Valeria , Stefanello Damiano , Ferrari Roberta , Zamboni Clarissa , Manfredi Martina , Giudice Chiara
Published: August 2023

Cytological evaluation of lymph nodes (LN) in canine cutaneous mast cell tumors (MCT) has a key role in MCT staging. However, cytological discrimination between metastatic and reactive LNs is debated and diagnostic criteria inconsistent. The aim of this study was to retrospectively quantify nodal mast cells (MCs) in non-oncological (NOD) and MCT-bearing dogs (MCTBD), using different sample preparation techniques, to evaluate the significance of the MCT number. Cytological specimens from NOD-LNs (10 fine-needle aspirates—FNAs) and MCTBD-LNs (10 FNAs, 10 scrapings, 10 touch imprints) were evaluated. MCTBD-LNs were grouped in: non-metastatic, possibly-metastatic, and metastatic based on current literature criteria. MCs were counted in 4, 8, and 20 high-power-fields, and over 500, 1000, and 2000 total cells. MCs were significantly more numerous in MCTBD-LNs than in NOD-LNs and in “metastatic” samples than in “non-metastatic”. There was no significant difference between “metastatic” and “possibly metastatic” samples. Sample preparation techniques did not influence these results. A negative correlation between MCs number and sample cellularity was observed. Results were confirmed regardless of the counting method applied. MCs counting per se cannot distinguish possibly metastatic and metastatic cytological samples. Sample preparation technique and the counting method applied seem to have no influence on cytological quantification of nodal MCs in MCTBDs.
In Vitro Evaluation of Antiviral Activities of Funicone-like Compounds Vermistatin and Penisimplicissin against Canine Coronavirus Infection
Authors: Cerracchio Claudia , Salvatore Maria Michela , Del Sorbo Luca , Serra Francesco , Amoroso Maria Grazia , DellaGreca Marina , Nicoletti Rosario , Andolfi Anna , Fiorito Filomena
Published: August 2023

Recent studies have demonstrated that 3-O-methylfunicone (OMF), a fungal secondary metabolite from Talaromyces pinophilus belonging to the class of funicone-like compounds, has antiviral activity against canine coronaviruses (CCoV), which causes enteritis in dogs. Herein, we selected two additional funicone-like compounds named vermistatin (VER) and penisimplicissin (PS) and investigated their inhibitory activity towards CCoV infection. Thus, both compounds have been tested for their cytotoxicity and for antiviral activity against CCoV in A72 cells, a fibrosarcoma cell line suitable for investigating CCoV. Our findings showed an increase in cell viability, with an improvement of morphological features in CCoV-infected cells at the non-toxic doses of 1 μM for VER and 0.5 μM for PS. In addition, we observed that these compounds caused a strong inhibition in the expression of the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor which is activated during CCoV infection. Our results also showed the alkalinization of lysosomes in the presence of VER or PS, which may be involved in the observed antiviral activities.
Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, Aplodinotus grunniens
Authors: Xue Miaomiao , Xu Pao , Wen Haibo , Chen Jianxiang , Wang Qingyong , He Jiyan , He Changchang , Kong Changxin , Song Changyou , Li Hongxia
Published: August 2023

The appropriate level of dietary lipids is essential for the nutrient requirements, rapid growth, and health maintenance of aquatic animals, while excessive dietary lipid intake will lead to lipid deposition and affect fish health. However, the symptoms of excessive lipid deposition in the liver of freshwater drums (Aplodinotus grunniens) remain unclear. In this study, a 4-month rearing experiment feeding with high-fat diets and a 6-week starvation stress experiment were conducted to evaluate the physiological alteration and underlying mechanism associated with lipid deposition in the liver of A. grunniens. From the results, high-fat-diet-induced lipid deposition was associated with increased condition factor (CF), viscerosomatic index (VSI), and hepatosomatic index (HSI). Meanwhile, lipid deposition led to physiological and metabolic disorders, inhibited antioxidant capacity, and exacerbated the burden of lipid metabolism. Lipid deposition promoted fatty acid synthesis but suppressed catabolism. Specifically, the transcriptome and metabolome showed significant enrichment of lipid metabolism and antioxidant pathways. In addition, the interaction analysis suggested that peroxisome proliferator-activated receptor (PPAR)-mediated 13-S-hydroxyoctadecenoic acid (13 (s)-HODE) could serve as the key target in regulating lipid metabolism and oxidative stress during lipid deposition in A. grunniens. Inversely, with a lipid intake restriction experiment, PPARs were confirmed to regulate lipid expenditure and physiological homeostasis in A. grunniens. These results uncover the molecular basis of and provide specific molecular targets for fatty liver control and prevention, which are of great importance for the sustainable development of A. grunniens.
Peroxiredoxins Play an Important Role in the Regulation of Immunity and Aging in Drosophila
Authors: Odnokoz Olena , Earland Noah , Badinloo Marziyeh , Klichko Vladimir , Benes Judith , Orr William , Radyuk Svetlana
Published: August 2023

Aberrant immune responses and chronic inflammation can impose significant health risks and promote premature aging. Pro-inflammatory responses are largely mediated via reactive oxygen species (ROS) and reduction–oxidation reactions. A pivotal role in maintaining cellular redox homeostasis and the proper control of redox-sensitive signaling belongs to a family of antioxidant and redox-regulating thiol-related peroxidases designated as peroxiredoxins (Prx). Our recent studies in Drosophila have shown that Prxs play a critical role in aging and immunity. We identified two important ‘hubs’, the endoplasmic reticulum (ER) and mitochondria, where extracellular and intracellular stress signals are transformed into pro-inflammatory responses that are modulated by the activity of the Prxs residing in these cellular organelles. Here, we found that mitochondrial Prx activity in the intestinal epithelium is required to prevent the development of intestinal barrier dysfunction, which can drive systemic inflammation and premature aging. Using a redox-negative mutant, we demonstrated that Prx acts in a redox-dependent manner in regulating the age-related immune response. The hyperactive immune response observed in flies under-expressing mitochondrial Prxs is due to a response to abiotic signals but not to changes in the bacterial content. This hyperactive response, but not reduced lifespan phenotype, can be rescued by the ER-localized Prx.
Salt Stress-Induced Modulation of Porphyrin Biosynthesis, Photoprotection, and Antioxidant Properties in Rice Plants (Oryza sativa)
Authors: Nguyen Anh Trung , Tran Lien Hong , Jung Sunyo
Published: August 2023

Salt stress disrupts cellular ion homeostasis and adversely impacts plant growth and productivity. We examined the regulatory mechanisms of porphyrin biosynthesis, photoprotection, and antioxidant properties in salt-stressed rice seedlings. In response to 150 mM NaCl, the rice seedlings exhibited dehydration, reduced relative water content, and increased levels of conductivity, malondialdehyde, and H2O2. The expression levels of the salt-stress-responsive genes NHX1, SOS1, and MYB drastically increased after NaCl treatment. The seedlings grown under NaCl stress displayed declines in Fv/Fm, ΦPSII, rETRmax, and photochemical quenching but increases in nonphotochemical quenching (NPQ) and the expression of genes involved in zeaxanthin formation, BCH, and VDE. Under salt stress conditions, levels of chlorophyll precursors significantly decreased compared to controls, matching the downregulation of CHLD, CHLH, CHLI, and PORB. By contrast, NaCl treatment led to increased heme content at 24 h of treatment and significant upregulations of FC2, HO1, and HO2 compared to controls. Salt-stressed seedlings also increased their expression of CATs (catalases) and APXs (ascorbate peroxidases) as well as the activities of superoxide dismutase, CAT, APX, and peroxidase. Our results indicate that chlorophyll and heme biosynthesis involve the protective strategies for salt stress alleviation through photoprotection by the scavenging of chlorophyll precursors and NPQ as well as activating antioxidant enzymes.
UAV-Assisted Caching Strategy Based on Content Cache Pricing in Vehicular Networks
Authors: Gong Ting , Zhu Qi
Published: August 2023

A UAV-assisted caching strategy considering content cache pricing in vehicular networks is proposed to address the problem of high communication load and high backhaul link overhead in vehicular networks. Consider a traffic scenario consisting of a content provider (CP), a network operator (NO), and multiple mobile users, where the NO has a set of cache-enabled roadside units (RSUs) and an unmanned aerial vehicle (UAV). The CP leases some popular contents to the NO for its benefit and the NO places this leased content in its RSU’s local cache to save expensive backhaul transmission overhead and latency. However, both NO and CP are selfish and their interests conflict with each other because they have opposing expectations for content pricing. In order to take into account the interests of both, this paper defines the utilities of CP and MNO and uses the Stackelberg game framework to model the competition between the two entities, where CP acts as a leader and sets the rental price of the content and NO acts as a follower responding to CP’s actions. An iteration-based dynamic programming algorithm is also designed to find the Stackelberg equilibrium. Meanwhile, a caching-capable UAV is introduced into the vehicular network and, based on this, a Dijkstra-based path planning algorithm is designed to further increase the total utility of NO by optimizing the trajectory of the UAV. The simulation results show that the strategy in this paper can reasonably allocate the benefits of CP and NO, reduce the average request delay, and increase the utility of NO; for example, we reduced the request latency for vehicle users by 27% and increased the total utility of NO by 13%.
Modeling Graph Neural Networks and Dynamic Role Sorting for Argument Extraction in Documents
Authors: Zhang Qingchuan , Chen Hongxi , Cai Yuanyuan , Dong Wei , Liu Peng
Published: August 2023

The existing methods for document-level event extraction mainly face two challenges. The first challenge is effectively capturing event information that spans across sentences. The second challenge is using predefined orders to extract event arguments while disregarding the dynamic adjusting of the order according to the importance of argument roles. To address these issues, we propose a model based on graph neural networks which realizes the semantic interaction among documents, sentences, and entities. Additionally, our model adopts a dynamic argument detection strategy, extracting arguments depending on their number in correspondence with each role. The experimental results confirm the outperformance of our model, which surpasses previous methods by 7% and 1.9% in terms of an F1 score.
Medical Application of Big Data: Between Systematic Review and Randomized Controlled Trials
Authors: Shim Sung Ryul , Lee Joon-Ho , Kim Jae Heon
Published: August 2023

In terms of medical health, we are currently living in the era of data science, which has brought tremendous change. Big data related to healthcare includes medical data, genome data, and lifelog data. Among medical data, public medical data is very important for actual research and medical policy reflection because it has data on a large number of patients and is representative. However, there are many difficulties in actually using such public health big data and designing a study, and conducting systematic review (SR) on the research topic can help a lot in the methodology. In this review, in addition to the importance of research using big data for the public interest, we will introduce important public medical big data in Korea and show how SR can be specifically applied in research using public medical big data.
Cytochalasans and Their Impact on Actin Filament Remodeling
Authors: Lambert Christopher , Schmidt Katharina , Karger Marius , Stadler Marc , Stradal Theresia E. B. , Rottner Klemens
Published: August 2023

The eukaryotic actin cytoskeleton comprises the protein itself in its monomeric and filamentous forms, G- and F-actin, as well as multiple interaction partners (actin-binding proteins, ABPs). This gives rise to a temporally and spatially controlled, dynamic network, eliciting a plethora of motility-associated processes. To interfere with the complex inter- and intracellular interactions the actin cytoskeleton confers, small molecular inhibitors have been used, foremost of all to study the relevance of actin filaments and their turnover for various cellular processes. The most prominent inhibitors act by, e.g., sequestering monomers or by interfering with the polymerization of new filaments and the elongation of existing filaments. Among these inhibitors used as tool compounds are the cytochalasans, fungal secondary metabolites known for decades and exploited for their F-actin polymerization inhibitory capabilities. In spite of their application as tool compounds for decades, comprehensive data are lacking that explain (i) how the structural deviances of the more than 400 cytochalasans described to date influence their bioactivity mechanistically and (ii) how the intricate network of ABPs reacts (or adapts) to cytochalasan binding. This review thus aims to summarize the information available concerning the structural features of cytochalasans and their influence on the described activities on cell morphology and actin cytoskeleton organization in eukaryotic cells.
The 3′UTR VNTR SLC6A3 Genetic Variant and Major Depressive Disorder: A Systematic Review
Authors: Gontijo Bruna Rodrigues , Possatti Isabella , Fratelli Caroline Ferreira , Pereira Alexandre Sampaio Rodrigues , Bonasser Larissa Sousa Silva , de Souza Silva Calliandra Maria , Rodrigues da Silva Izabel Cristina
Published: August 2023

Major Depressive Disorder (MDD) is a disabling and particularly persistent mental disorder that is considered to be a priority public health problem. The active human dopamine transporter (DAT), which is encoded by the SLC6A3 gene, regulates the dopamine concentration in the synaptic cleft. In this sense, this neurotransmitter is primordial in modulating human emotions. This systematic review aims to verify the SLC6A3 (DAT1) 3′UTR VNTR (rs28363170) gene variant’s SS (9R/9R) genotype and S (9R) allele frequency fluctuation and its influence on the modulation of pharmacotherapy in MDD. For this purpose, we searched different databases, and after applying the eligibility criteria, six articles were selected. Studies have shown an association between the SS (9R/9R) genotypic and S (9R) allelic presence with the risk of developing MDD, in addition to influencing the decrease in response to antidepressant therapy. However, despite the findings, disagreements were observed between other studies. For this reason, further studies with the SLC6A3 3′UTR VNTR (rs28363170) variant in different populations are necessary to understand this polymorphism’s role in the onset of this disease.
Colorimetric and Label-Free Optical Detection of Pb2+ Ions via Colloidal Gold Nanoparticles
Authors: Flowers Jasmin A. , Farrell Monique J. , Rutherford Gugu , Pradhan Aswini K.
Published: August 2023

The detection of the lead heavy metal (Pb) in water is crucial in many chemical processes, as it is associated with serious health hazards. Here, we report the selective and precise colorimetric detection of Pb2+ ions in water, exploiting the aggregation and self-assembly mechanisms of glutathione (GSH)-functionalized gold nanoparticles (GNPs). The carboxyl functional groups are able to create coordination complexes with Pb2+, inducing aggregation amongst the GSH-GNPs in the presence of Pb2+ due to the chelation of the GSH ligands. The resulting aggregation amongst the GSH-GNPs in the presence of Pb2+ increases the aggregate size depending on the available Pb2+ ions, affecting the plasmonic coupling. This causes a substantial shift in the plasmon wavelength to a longer wavelength side with increasing Pb2+ concentration, resulting in a red-to-blue colorimetric or visual change, enabling the instant determination of lead content in water.
Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection
Authors: Yuwen Lihui , Zhang Shifeng , Chao Jie
Published: August 2023

Virus-related infectious diseases are serious threats to humans, which makes virus detection of great importance. Traditional virus-detection methods usually suffer from low sensitivity and specificity, are time-consuming, have a high cost, etc. Recently, DNA biosensors based on DNA nanotechnology have shown great potential in virus detection. DNA nanotechnology, specifically DNA tiles and DNA aptamers, has achieved atomic precision in nanostructure construction. Exploiting the programmable nature of DNA nanostructures, researchers have developed DNA nanobiosensors that outperform traditional virus-detection methods. This paper reviews the history of DNA tiles and DNA aptamers, and it briefly describes the Baltimore classification of virology. Moreover, the advance of virus detection by using DNA nanobiosensors is discussed in detail and compared with traditional virus-detection methods. Finally, challenges faced by DNA nanobiosensors in virus detection are summarized, and a perspective on the future development of DNA nanobiosensors in virus detection is also provided.
Modern Aspects of the Complex Interactions between Biodiversity and the Main Neuropsychiatric Disorders
Authors: Borca Madalina , Ciobica Alin , Iordache Alin
Published: August 2023

The high incidence of psychopathologies recorded in today’s human society, correlated with the high percentages of biodiversity loss, point to the need for an interdisciplinary approach of the scientific fields under study—neuroscience and biodiversity conservation. Thus, our approach here presents, in a synergistic manner, the significant correlation between mental health and the increased values of biodiversity in the ecosystems located in the immediate vicinity, especially those located in the middle of cities. Our approach aims to emphasize the importance of biodiversity conservation in the context of preserving mental health and general well-being. There are a series of recent experimental demonstrations that outline the influence of natural elements on the human psyche and, implicitly, the effects of nature in the prevention and reduction of stress, anxiety, and depression. And beyond the cognitive barriers of humanity in relating to the surrounding biodiversity must lie the desire to know the values of biodiversity and the absolute importance of its conservation. The sustainable relationship between humans and living nature, seen as a complex of biodiversity, is dealt with by a branch of science called human ecology. Therefore, this study emphasizes the crucial need to know and respect the connection between man and nature, based, since time immemorial, on biophilia. And with the regression of ignorance and the correlated approach of several scientific fields, some at the intersection of the humanities and natural sciences, one can observe the progress of preserving the dynamic balance within ecosystems and, implicitly, the preservation of mental health and human well-being.
Reassessing the Proportional System of Joseon Era Wooden Architecture: The Bracket Arm Length and Width as a Standard Modular Method
Authors: Cha Ju-Hwan , Kim Young-Jae
Published: August 2023

Previous research has agreed that standard modular methods in Song Yingzao Fashi and Qing Gongcheng Zuofa were not applied to Korean wooden architecture. This study notes the size of bracket arms as a standard modular method by investigating the proportion systems of Sungnyemun, Paldalmun, and Heunginjimun Gates, the official government buildings of the Joseon Dynasty. The purlin direction bracket arms in the intercolumnar bracket sets apply a proportional system in the ratio of a regular integer relationship to the front and side facades and building height. Challenging current assumptions, the application of the bracket arm width as a modular rule is divided into more subdivided values than the measurement units. A particularly important finding is that, unlike the height of the bracket arms, the width and length of the brackets are standard members that determine the height of the side facades. This is very similar to the official government building styles in the Song and Qing Dynasties, premodern China. Therefore, this study is meaningful in reassessing wooden frame structures of the Joseon era, deriving parametric measuring rules universally applied in East Asia to provide basic data useful for heritage conservation.
Comparative Assessment of Different Ultrasound Technologies in the Detection of Prostate Cancer: A Systematic Review and Meta-Analysis
Authors: Alghamdi Dareen , Kernohan Neil , Li Chunhui , Nabi Ghulam
Published: August 2023

The present study aimed to assess the diagnostic test accuracy of different ultrasound scanning technologies in the detection of prostate cancer. A systematic search was conducted using the Cochrane Guidelines for Screening and Diagnostic Tests. We performed a systematic search in the international databases PubMed, Medline, Ovid, Embase and Cochrane Library. Searches were designed to find all studies that evaluated Micro-US, mpUS, SWE and CEUS as the main detection modalities for prostate cancer. This study was registered with Research Registry of systematic review and meta-analysis. The QUADAS-2 tool was utilized to perform quality assessment and bias analysis. The literature search generated 1376 studies. Of these, 320 studies were screened for eligibility, with 1056 studies being excluded. Overall, 26 studies with a total of 6370 patients met the inclusion criteria. The pooled sensitivity for grayscale, CEUS, SWE, Micro-US and mpUS modalities were 0.66 (95% CI 0.54–0.73) 0.73 (95% CI 0.58–0.88), 0.82 (95% CI 0.75–0.90), 0.85 (95% CI 0.76–0.94) and 0.87 (95% CI 0.71–1.03), respectively. Moreover, the pooled specificity for grayscale, CEUS, SWE, Micro-US and mpUS modalities were 0.56 (95% CI 0.21–0.90), 0.78 (95% CI 0.67–0.88), 0.76 (95% CI 0.65–0.88), 0.43 (95% CI 0.28–0.59) and 0.68 (95% CI 0.54–0.81), respectively. In terms of sensitivity, substantial heterogeneity between studies was detected (I2 = 72%, p = 0.000 < 0.05). In relation to specificity, extreme heterogeneity was detected (I2 = 93%, p = 0.000 < 0.05). Some studies proved that advanced ultrasound modalities such as mpUS, Micro-US, shear-wave elastography, contrast enhanced and micro-ultrasound are promising methods for the detection of prostate cancer.
Macroscopic Evaluation of Colon Cancer Resection Specimens
Authors: Jarrett Ross , West Nicholas P.
Published: August 2023

Colon cancer is a common disease internationally. Outcomes have not improved to the same degree as in rectal cancer, where the focus on total mesorectal excision and pathological feedback has significantly contributed to improved survival and reduced local recurrence. Colon cancer surgery shows significant variation around the world, with differences in mesocolic integrity, height of the vascular ligation and length of the bowel resected. This leads to variation in well-recognised quality measures like lymph node yield. Pathologists are able to assess all of these variables and are ideally placed to provide feedback to surgeons and the wider multidisciplinary team to improve surgical quality over time. With a move towards complete mesocolic excision with central vascular ligation to remove the primary tumour and all mechanisms of spread within an intact package, pathological feedback will be central to improving outcomes for patients with operable colon cancer. This review focusses on the key quality measures and the evidence that underpins them.
Mechanistic Insight into the Propane Oxidation Dehydrogenation by N2O over Cu-BEA Zeolite with Diverse Active Site Structures
Authors: Wu Ruiqi , Liu Ning , Dai Chengna , Xu Ruinian , Yu Gangqiang , Wang Ning , Chen Biaohua
Published: August 2023

The present work theoretically investigated propane oxidation dehydrogenation by utilizing N2O as an oxidant (N2O-ODHP) over Cu-BEA with three different types of active site, including monomeric Cu ([Cu]+), dimeric Cu ([Cu−Cu]2+), and distant monomeric Cu sites ([Cu]+—[Cu]+). Energetically, we calculated that the monomeric [Cu]+ is favorable for the αH dehydrogenation step (∆E = 0.05 eV), which, however, suffers from high barriers of N2O dissociation and βH dehydrogenation steps of 1.40 and 1.94 eV, respectively. Although the dimeric [Cu−Cu]2+ site with a Cu—Cu distance of 4.91 Å is much more favorable for N2O dissociation (0.95 eV), it still needs to overcome an extremely high barrier (∆E = 2.15 eV) for βH dehydrogenation. Interestingly, the distant [Cu]+—[Cu]+ site with the Cu—Cu distance of 5.82 Å exhibits low energy barriers for N2O dissociation (0.89 eV) and ODHP steps (0.01 and 0.33 eV) due to the synergistic effect of distant [Cu]+. The microkinetic analyses quantitatively verified the superior activity of the distant [Cu]+—[Cu]+ site with a reaction rate being eight to nine orders of magnitude higher than those of the monomeric and the dimeric Cu sites, and this is related to its ready charge-transfer ability, as shown by the partial Density of State (PDOS) analysis and the static charge differential density analysis in this study. Generally, the present work proposes that the distance between the [Cu]+ sites plays a significant and important role in N2O-ODHP over the Cu-based zeolite catalyst and modulates Cu—Cu distance, and this constitutes a promising strategy for highly-efficient Cu-zeolite catalyst design for N2O-ODHP.
Wearable Chemosensors in Physiological Monitoring
Authors: Tang Zeyi , Cui Tianrui , Liu Houfang , Jian Jinming , Li Ding , Yang Yi , Ren Tianling
Published: August 2023

The development of flexible electronic technology has led to significant advancements in wearable sensors. In the past decades, wearable chemosensors have received much attention from researchers worldwide due to their high portability, flexibility, lightweight, and adaptability. It allows real-time access to the user’s physiological status at the molecular level to analyze their health status. Therefore, it can be widely used in the field of precision medicine. This review introduces the sensing mechanisms of wearable chemosensors and recent progress in wearable sweat and interstitial fluid-based chemosensors. The complexities of wearable chemosensors are not to be underestimated, as there are considerable challenges in this field. This review aims to shed light on the difficulties associated with designing wearable sweat and interstitial fluid-based chemosensors and their potential development directions.
Gas-Sensing Properties of Graphene Functionalized with Ternary Cu-Mn Oxides for E-Nose Applications
Authors: Kodu Margus , Pärna Rainer , Avarmaa Tea , Renge Indrek , Kozlova Jekaterina , Kahro Tauno , Jaaniso Raivo
Published: August 2023

Chemiresistive gas sensors were produced by functionalizing graphene with a ~3 nm layer of mixed oxide xCu2O⸱yMnO using pulsed laser deposition (PLD) from a hopcalite CuMn2O4 target. Sensor response time traces were recorded for strongly oxidizing (NO2, O3) and reducing (NH3, H2S) poisonous gases at ppb and ppm levels, respectively. The morphology of the MOX layer was modified by growth temperature during PLD, resulting in the optimization of the sensor response. Differences in decomposition or oxidation rates on catalytically active metal oxide (MOX) were utilized to achieve partial selectivity for pairs of gases that have similar adsorption and redox properties. The predominant selectivity towards ozone in most samples at different measuring conditions remained difficult to suppress. A distinct selectivity for H2S emerged at higher measurement temperatures (100–150 °C), which was assigned to catalytic oxidation with O2. Several gas–MOX interaction mechanisms were advanced to tentatively explain the sensor behavior, including reversible electron transfer in the simplest case of NO2, decomposition via ionic transients for O3, and complex catalytic oxidative transformations for NH3 and H2S.
A Comparison of Slow Infusion Intermittent Feeding versus Gravity Feeding in Preterm Infants: A Randomized Controlled Trial
Authors: Yavanoglu Atay Funda , Bozkurt Ozlem , Sahin Suzan , Bidev Duygu , Sari Fatma Nur , Uras Nurdan
Published: August 2023

Background: The transition to full enteral feeding is important for ensuring adequate growth in preterm infants. Aims: The aim of this study was to investigate the effects of two different intermittent feeding methods on the transition to full enteral feeding in preterm infants. Study design: A prospective, randomized controlled study was conducted in a neonatology and perinatology center. Subjects: Preterm infants with a gestational age between 24 + 0/7 and 31 + 6/7 were included in this study. They were divided into two groups: the SIF (slow infusion feeding) group and the IBF (intermittent bolus feeding) group. In the SIF group, feed volumes were administered over one hour using an infusion pump through an orogastric tube, with feeding occurring every three hours. The IBF group received enteral feeding using a gravity-based technique with a syringe through an orogastric tube, completed within 10 to 30 min. Outcome measures: The primary outcome was the achievement of full enteral feeding and the occurrence of feeding intolerance. Results: A total of 103 infants were enrolled in the study (50 in SIF and 53 in IBF). The time to achieve full enteral feeding did not differ significantly between the two groups (p = 0.20). The SIF group had significantly fewer occurrences in which gastric residual volume exceeded 50% (p = 0.01). Moreover, the SIF group had a significantly shorter duration of non-per-oral (NPO) status than the IBF group (p = 0.03). Conclusions: It is our contention that the use of the SIF method as an alternative feeding method is appropriate for infants with feeding intolerance and those at high risk of feeding intolerance.
The Relationship between Narrative Skills and Executive Functions across Childhood: A Systematic Review and Meta-Analysis
Authors: Scionti Nicoletta , Zampini Laura , Marzocchi Gian Marco
Published: August 2023

Executive functions (EFs) and narrative competence (NC) are two important predictors of many outcomes in human development. To date, however, it is unclear whether these skills develop synergistically—supporting or opposing each other—or whether they are independent of each other. The purpose of this meta-analysis is to understand if these skills are related to over development and if the magnitude of their association changes over time; differs in typical and atypical development; and changes with EF (inhibition, working memory, flexibility, planning) and NC (oral, written; micro and macrostructural level). For this purpose, 30 studies containing 285 effect sizes were selected and combined. The results show that EFs and NC are weakly associated with each other (r = 0.236, p < 0.001) and that this association decreases with age (b(267) = −0.0144, p = 0.001). They are more associated in preschool and early elementary school grades, becoming more independent after seven years old. Between 3 and 7 years of age, the association seems stronger in atypically developing children and for macrostructural NC. Additionally, before 7 years old, the various EF domains seem to associate indistinctly with NC, and only later specific links between EFs and NC would be observed.
Relevant Properties and Potential Applications of Sericin in Bone Regeneration
Authors: Kim Jwa-Young , Kim Seong-Gon , Garagiola Umberto
Published: August 2023

The potential of sericin, a protein derived from silkworms, is explored in bone graft applications. Sericin’s biocompatibility, hydrophilic nature, and cost-effectiveness make it a promising candidate for enhancing traditional graft materials. Its antioxidant, anti-inflammatory, and UV-resistant properties contribute to a healthier bone-healing environment, and its incorporation into 3D-printed grafts could lead to personalized medical solutions. However, despite these promising attributes, there are still gaps in our understanding. The precise mechanism through which sericin influences bone cell growth and healing is not fully understood, and more comprehensive clinical trials are needed to confirm its long-term biocompatibility in humans. Furthermore, the best methods for incorporating sericin into existing graft materials are still under investigation, and potential allergic reactions or immune responses to sericin need further study.
Anti-Inflammatory Effects of Serotonin Receptor and Transient Receptor Potential Channel Ligands in Human Small Intestinal Epithelial Cells
Authors: Robinson Gregory Ian , Li Dongping , Wang Bo , Zahoruiko Yeva , Gerasymchuk Marta , Hudson Darryl , Kovalchuk Olga , Kovalchuk Igor
Published: August 2023

Intestinal inflammation and dysbiosis can lead to inflammatory bowel diseases (IBD) and systemic inflammation, affecting multiple organs. Developing novel anti-inflammatory therapeutics is crucial for preventing IBD progression. Serotonin receptor type 2A (5-HT2A) ligands, including psilocybin (Psi), 4-Acetoxy-N,N-dimethyltryptamine (4-AcO-DMT), and ketanserin (Ket), along with transient receptor potential (TRP) channel ligands like capsaicin (Cap), curcumin (Cur), and eugenol (Eug), show promise as anti-inflammatory agents. In this study, we investigated the cytotoxic and anti-inflammatory effects of Psi, 4-AcO-DMT, Ket, Cap, Cur, and Eug on human small intestinal epithelial cells (HSEIC). HSEIC were exposed to tumor necrosis factor (TNF)-α and interferon (IFN)-γ for 24 h to induce an inflammatory response, followed by treatment with each compound at varying doses (0–800 μM) for 24 to 96 h. The cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and protein expression by Western blot (WB) analysis. As single treatments, Psi (40 μM), Cur (0.5 μM), and Eug (50 μM) significantly reduced COX-2 levels without cytotoxic effects. When combined, Psi (40 μM) and Cur (0.5 μM) exhibited synergy, resulting in a substantial decrease in COX-2 protein levels (−28× fold change), although the reduction in IL-6 was less pronounced (−1.6× fold change). Psi (20 μM) and Eug (25 μM) demonstrated the most favorable outcomes, with significant decreases in COX-2 (−19× fold change) and IL-6 (−10× fold change) protein levels. Moreover, the combination of Psi and Eug did not induce cytotoxic effects in vitro at any tested doses. This study is the first to explore the anti-inflammatory potential of psilocybin and 4-AcO-DMT in the intestines while highlighting the potential for synergy between the 5-HT2A and TRP channel ligands, specifically Psi and Eug, in alleviating the TNF-α/IFN-γ-induced inflammatory response in HSEIC. Further investigations should evaluate if the Psi and Eug combination has the therapeutic potential to treat IBD in vivo.
The Effect of Coating Additives on the Properties of Hydrophobic Coatings with Low Infrared Emissivity
Authors: Zhang Weigang , Li Xiang , Lv Dandan
Published: August 2023

In order to further reduce the emissivity of hydrophobic low infrared emissivity composite coating and improve its mechanical properties, a dispersant, adhesion enhancer, and defoamer were used to improve the dispersion state of the fillers, the interface structure, and the surface state of a hydrogen silicone oil-modified polyurethane/Al composite coating. The influences of dispersant, adhesion enhancer, and defoamer on the microstructure, emissivity, glossiness, hydrophobic property, adhesion strength, and impact strength of the coating were systematically studied. The results indicate that polycarboxylate anionic dispersant can improve the dispersion state of Al powder and nano-SiO2 in the coating, so that the infrared emissivity of the coating can be reduced, and the coating can have greater hydrophobic and mechanical properties. The bonding strength between the resin and the substrate of the coating can be improved by the adhesion enhancer through a bridging action, so that the mechanical properties of the coating can be significantly improved. The defoamer can significantly reduce the pores in the coating, resulting in a substantial improvement in the surface condition, and the mechanical properties can be significantly improved. The coating exhibits superior characteristics, including an emissivity value of 0.527, glossiness of 4.3, adhesion strength of grade 1, impact strength of 40 kg·cm, and hydrophobic property (the water contact angle (WCA) is 140°) when the amount of dispersant, adhesion enhancer, and defoamer is 5 wt%, 4 wt%, and 1 wt%, respectively.
Numerical Simulation and Performance Optimization of a Solar Cell Based on WO3/CdTe Heterostructure Using NiO as HTL Layer by SCAPS 1D
Authors: Medina José Carlos Zepeda , Andrés Enrique Rosendo , Ruíz Crisóforo Morales , Espinosa Eduardo Camacho , Yarce Leticia Treviño , Galeazzi Isasmendi Reina , Trujillo Román Romano , Salgado Godofredo García , Solis Antonio Coyopol , Caballero Fabiola Gabriela Nieto
Published: August 2023

In this paper, a solar cell based on WO3/CdTe heterojunction was analyzed and optimized, for which the following structure of the Al/AZO/WO3/CdTe/NiO/Ni device was proposed, which was numerically simulated by the SCAPS 1-D software. Using the software, the effect of the thickness and carrier concentration of the absorber layer (CdTe) and the window layer (WO3) was analyzed, and the optimal value of these parameters was found to be 2 µm and 1015 cm−3 for the CdTe layer and 10 nm and 1019 cm−3 for the WO3 layer, respectively. The influence of the defect density of the WO3/CdTe interface on the performance of the proposed cell was also analyzed, simulating from 1010 to 1016 cm−2, obtaining better device performance at lower interface defect density. Another parameter analyzed was the operating temperature on the photovoltaic performance of the device, observing that the solar cell has a better performance at lower temperatures. Finally, a maximum optimized PCE of 19.87% is obtained with a Voc = 0.85 V, Jsc = 28.45 mA/cm2, and FF = 82.03%, which makes the WO3/CdTe heterojunction an interesting alternative for the development of CdTe-based solar cells.
Substrate Structured Bournonite CuPbSbS3 Thin Film Solar Cells
Authors: Lin Limei , Da Rui , Zheng Chenqi , Zeng Ruibo , Ding Junda
Published: August 2023

CuPbSbS3 has excellent photoelectric properties, such as high element abundance and optical absorption coefficient, and a suitable band gap, which is a material with the potential for absorbing layers of high-quality thin film solar cells. In addition, CuPbSbS3 is a material with a three-dimensional structure, which can guide the carrier to transport in all directions, so its performance can be regulated in multiple dimensions. At present, the substrate structure is often used in efficient solar cells since this structure does not affect other functional layers when the absorption layer is subjected to harsh annealing conditions. However, there have been no reports of the substrate structure of CuPbSbS3 solar cells so far. Therefore, in this work, CuPbSbS3 films deposited on a stable substrate of molybdenum (Mo) were prepared with butyldithiocarbamic acid (BDCA) solution, and the preparation process of reaction mechanism was described in detail. It was found that the band gap of the CuPbSbS3 thin film was 2.0 eV and the absorption coefficient was up to 105 cm−1, which is expected to be applied to the top absorption layer material in laminated cells. Thus, we first built a Glass/Mo/CuPbSbS3/CdS/ZnO/ITO substrate structured solar cell. From this, a photoelectric conversion efficiency of 0.094% was achieved. This work provides a tentative exploration for the future development of substrate structured CuPbSbS3 solar cells.
Pleistocene Aquatic Refuges Support the East–West Separation of the Neotropical Catfish Trichomycterinae (Siluriformes: Trichomycteridae) and High Diversity in the Magdalena, Guiana, and Paraná-Paraguay Basins
Authors: Volpi Thais de Assis , Monjardim Marina , Sarmento-Soares Luisa M. , Fagundes Valéria
Published: August 2023

(1) Background: Trichomycterinae represent 60% of the species in the family and, while seven genera comprise 1–3 species each, Trichomycterus and Cambeva have over 180 known species between them. Although integrative studies aimed to clarify the relationships within the subfamily, the diversity of species of Trichomycterus remains an open question. Herein, we explored an unprecedented sample to investigate the divergence in the lineages of Trichomycterus. (2) Methods: we recovered the phylogenetic relationships of the subfamily using 566 sequences (999 bp) of the mitochondrial gene cytochrome b, calculated intra- and intergroup distance percentages, and estimated divergence times. (3) Results: we recovered 13 highly supported and geographically structured lineages; intergenus divergence was 11–20%, while interspecies divergence was 3–11%; Trichomycterus, Cambeva, Scleronema, Hatcheria, Eremophilus, and Ituglanis were recovered as monophyletic, with three other highly divergent clades: Guiana Shield, Magdalena basin, and Tapajós basin. (4) Conclusions: We propose that the trans-Andean austral clades be allocated into Hatcheria, and the Guiana clade supports a new genus. We also observed that the headwaters nearest the Magdalena and Orinoco basins showed a high diversity and endemism of Trichomycterinae lineages. We discussed the role of geomorphological events and the climatic features which may explain cladogenesis events in Trichomycterinae.
Pembrolizumab-Induced Lichen Planus Pemphigoides in a Patient with Metastatic Adrenocortical Cancer: A Case Report and Literature Review
Authors: Madan Vrinda , Marchitto Mark C. , Sunshine Joel C.
Published: August 2023

While the advent of immune-checkpoint inhibitors has revolutionized cancer therapy, immune-related adverse effects (irAEs) have also been on the rise. Cutaneous toxicities are among the most common irAEs, especially in the context of programmed cell death protein-1 (PD-1) inhibitors like pembrolizumab. Herein, we report a case of anti-PD-1-induced lichen planus pemphigoides (LPP)—a rare autoimmune blistering disorder with characteristics of both lichen planus and bullous pemphigoid. To our knowledge, this is the first reported case of LPP following anti-PD-1 therapy for metastatic adrenocortical cancer. Recognizing that LPP is within the spectrum of irAEs is important, especially as the indications for immunotherapy grow to include rarer malignancies like adrenocortical cancer. In addition to our case presentation, we also provide a comprehensive review of the literature surrounding immunotherapy-induced LPP—highlighting key characteristics towards the early recognition and clinical management of this cutaneous irAE.
The Impact of Prostate Volume on the Prostate Imaging and Reporting Data System (PI-RADS) in a Real-World Setting
Authors: Volz Yannic , Apfelbeck Maria , Pyrgidis Nikolaos , Pfitzinger Paulo L. , Berg Elena , Ebner Benedikt , Enzinger Benazir , Ivanova Troya , Atzler Michael , Kazmierczak Philipp M. , Clevert Dirk-André , Stief Christian , Chaloupka Michael
Published: August 2023

Multiparametric magnetic resonance imaging (mpMRI) has emerged as a new cornerstone in the diagnostic pathway of prostate cancer. However, mpMRI is not devoid of factors influencing its detection rate of clinically significant prostate cancer (csPCa). Amongst others, prostate volume has been demonstrated to influence the detection rates of csPCa. Particularly, increasing volume has been linked to a reduced cancer detection rate. However, information about the linkage between PI-RADS, prostate volume and detection rate is relatively sparse. Therefore, the current study aims to assess the association between prostate volume, PI-RADS score and detection rate of csP-Ca, representing daily practice and contemporary mpMRI expertise. Thus, 1039 consecutive patients with 1151 PI-RADS targets, who underwent mpMRI-guided prostate biopsy at our tertiary referral center, were included. Prior mpMRI had been assessed by a plethora of 111 radiology offices, including academic centers and private practices. mpMRI was not secondarily reviewed in house before biopsy. mpMRI-targeted biopsy was performed by a small group of a total of ten urologists, who had performed at least 100 previous biopsies. Using ROC analysis, we defined cut-off values of prostate volume for each PI-RADS score, where the detection rate drops significantly. For PI-RADS 4 lesions, we found a volume > 61.5 ccm significantly reduced the cancer detection rate (OR 0.24; 95% CI 0.16–0.38; p < 0.001). For PI-RADS 5 lesions, we found a volume > 51.5 ccm to significantly reduce the cancer detection rate (OR 0.39; 95% CI 0.25–0.62; p < 0.001). For PI-RADS 3 lesions, none of the evaluated clinical parameters had a significant impact on the detection rate of csPCa. In conclusion, we show that enlarged prostate volume represents a major limitation in the daily practice of mpMRI-targeted biopsy. This study is the first to define exact cut-off values of prostate volume to significantly impair the validity of PI-RADS assessed in a real-world setting. Therefore, the results of mpMRI-targeted biopsy should be interpreted carefully, especially in patients with prostate volumes above our defined thresholds.