bluuebunny/crossref_metadata_2025 huggingface dataset:
COLMENA: A Culture Collection of Native Microorganisms for Harnessing the Agro-Biotechnological Potential in Soils and Contributing to Food Security
Authors: de los Santos-Villalobos Sergio , Díaz-Rodríguez Alondra María , Ávila-Mascareño María Fernanda , Martínez-Vidales Andrea Denisse , Parra-Cota Fannie Isela
Published: July 2021

COLMENA is a microbial culture collection dedicated to the characterization, classification, preservation, and transferal of native microorganisms isolated from various agro-systems and other ecosystems in Mexico. This collection aims to protect microbial diversity, reducing soil degradation, but also exploiting its agro-biotechnological potential. So far, COLMENA has isolated and cryopreserved soil microorganisms from different crops in two major agricultural regions in Mexico, the Yaqui Valley, Sonora, and the Fuerte Valley, Sinaloa. COLMENA has specialized in the identification and characterization of microbial strains with metabolic capacities related to the promotion of plant growth and the biocontrol of phytopathogens. Thus, COLMENA has identified several promising plant growth-promoting microbial (PGPM) strains due to their metabolic and genetic potentials and their beneficial effects in vivo and field trials. These findings demonstrate the biotechnological potential of these strains for their future use in profitable agricultural alternatives focused on enhancing global food security. To share the knowledge and results of the COLMENA team’s scientific research, a virtual platform was created, where the database of the studied and preserved microorganisms is available to professionals, researchers, agricultural workers, and anyone who is interested.
Conserved Sequence Analysis of Influenza A Virus HA Segment and Its Application in Rapid Typing
Authors: Lin Qianyu , Ji Xiang , Wu Feng , Ma Lan
Published: July 2021

The high mutation rate of the influenza A virus hemagglutinin segment poses great challenges to its long-term effective testing and subtyping. Our conserved sequence searching method achieves high-specificity conserved sequences on H1–H9 subtypes. In addition, PCR experiments show that primers based on conserved sequences can be used in influenza A virus HA subtyping. Conserved sequence-based primers are expected to be long-term, effective subtyping tools for influenza A virus HA.
The Incremental Prognostic Value of E/(e’×s’) Ratio in Non-ST-Segment Elevated Acute Coronary Syndrome
Authors: Ionac Ioana , Lazăr Mihai-Andrei , Brie Daniel , Erimescu Constantin , Vînă Radu , Mornoş Cristian
Published: July 2021

It has been shown that the E/(e’×s’) index, which associates a marker of diastolic function (E/e’, early transmitral/diastolic mitral annulus velocity ratio) and a parameter that explores LV systolic performance (s’, systolic mitral annulus velocity), is a good predictor of outcome in acute anterior myocardial infarction. There are no studies that have investigated the prognostic value of E/(e’×s’) in a non-ST-segment elevated acute coronary syndrome (NSTE-ACS) population. Echocardiography was performed in 307 consecutive hospitalized patients with NSTE-ACS and succesful percutaneous coronary intervention, before discharge and six weeks after. The primary endpoint consisted of cardiac death or readmission due to re-infarction or heart failure. During the follow-up period (25.4 ± 3 months), cardiac events occurred in 106 patients (34.5%). Receiver operating characteristic (ROC) analysis identified E/(e’×s’) at discharge as the best independent predictor of composite outcome. The optimal cut-off value was 1.63 (74% sensitivity, 67% specificity). By multivariate Cox regression analysis, E/(e’×s’) was the only independent predictor of cardiac events. Kaplan–Meier analysis identified that patients with an initial E/(e’×s’) > 1.63 that worsened after six weeks presented the worst prognosis regarding composite outcome, readmission, and cardiac death (all p < 0.001). In conclusion, in NSTE-ACS, E/(e’×s’) is a powerful predictor of clinical outcome, particularly if it is accompanied by worsening after 6-weeks.
Design Techniques for Low-Power and Low-Voltage Bandgaps
Authors: Barteselli Edoardo , Sant Luca , Gaggl Richard , Baschirotto Andrea
Published: July 2021

Reverse bandgaps generate PVT-independent reference voltages by means of the sums of pairs of currents over individual matched resistors: one (CTAT) current is proportional to VEB; the other one (PTAT) is proportional to VT (Thermal voltage). Design guidelines and techniques for a CMOS low-power reverse bandgap reference are presented and discussed in this paper. The paper explains firstly how to design the components of the bandgap branches to minimize circuit current. Secondly, error amplifier topologies are studied in order to reveal the best one, depending on the operation conditions. Finally, a low-voltage bandgap in 65 nm CMOS with 5 ppm/°C, with a DC PSR of −91 dB, with power consumption of 5.2 μW and with an area of 0.0352 mm2 developed with these techniques is presented.
Nonsingular Terminal Sliding Mode Control of PMSM Based on Improved Exponential Reaching Law
Authors: Jiang Changhong , Wang Qiming , Li Zonghao , Zhang Niaona , Ding Haitao
Published: July 2021

When a permanent magnet synchronous motor runs at low speed, the inverter will output discontinuous current and generate torque ripple; when the motor is runs at high speed, a large amount of stator harmonic current generates, which affects its speed following ability and torque stability. To ensure the fast and smooth switching of a permanent magnet synchronous motor in the full speed domain, this paper proposes the nonsingular terminal sliding mode control of PMSM speed control based on the improved exponential reaching law. Firstly, the improved exponential reaching law is composed of the state variables and power terms of the sliding mode surface functions. The reaching law function is designed in sections to balance the fast dynamic response of the system and chattering control. Secondly, an improved exponential reaching law based on the sliding mode control strategy of the PMSM speed loop is proposed. By designing the initial value of the integral term in the nonsingular terminal sliding mode surface function, the initial state of the system is located on the sliding mode surface. The integral sliding mode surface is used to reduce the system steady-state error, while the proposed sliding mode reaching law is used to increase the arrival speed and suppress system chattering, ultimately affecting modeling error problems, complex working conditions, and uncertainty factors. This paper proposes a sliding mode observer based on an improved exponential reaching law to compensate for the disturbances. Lyapunov stability theory can prove that this system can make the speed tracking error converge to zero in finite time. Hardware-in-the-loop experiments were used to validate the effectiveness of the proposed method.
Adsorption of Estradiol by Natural Clays and Daphnia magna as Biological Filter in an Aqueous Mixture with Emerging Contaminants
Authors: Pérez-González Andrés , Pinos-Vélez Verónica , Cipriani-Avila Isabel , Capparelli Mariana , Jara-Negrete Eliza , Alvarado Andrés , Cisneros Juan Fernando , Tripaldi Piercosimo
Published: July 2021

Among emerging pollutants, endocrine disruptors such as estradiol are of most concern. Conventional water treatment technologies are not capable of removing this compound from water. This study aims to assess a method that combines physicochemical and biological strategies to eliminate estradiol even when there are other compounds present in the water matrix. Na-montmorillonite, Ca-montmorillonite and zeolite were used to remove estradiol in a medium with sulfamethoxazole, triclosan, and nicotine using a Plackett–Burman experimental design; each treatment was followed by biological filtration with Daphnia magna. Results showed between 40 to 92% estradiol adsorption in clays; no other compounds present in the mixture were adsorbed. The most significant factors for estradiol adsorption were the presence of nicotine and triclosan which favored the adsorption, the use of Ca-montmorillonite, Zeolite, and time did not favor the adsorption of estradiol. After the physicochemical treatment, Daphnia magna was able to remove between 0–93% of the remaining estradiol. The combination of adsorption and biological filtration in optimal conditions allowed the removal of 98% of the initial estradiol concentration.
Increased Post-Drought Growth after Thinning in Pinus nigra Plantations
Authors: Manrique-Alba Àngela , Beguería Santiago , Tomas-Burguera Miquel , Camarero Jesús Julio
Published: July 2021

In Mediterranean seasonally dry regions, the rise in dieback and mortality episodes observed in pine afforestations has been related to higher drought intensity and lack of appropriate management, which enhance competition between trees for water and light. However, there is little understanding of the benefits of silviculture for plantations under seasonal drought stress. A combination of dendrochronology and wood C and O isotope analyses was used in three Black pine (Pinus nigra) plantations to work out the responses of radial growth (BAI, basal area increment) and water-use efficiency (WUEi) to thinning treatments (removal of 40% of the stand basal area). Thinning had a positive effect on BAI and WUEi, reduced drought sensitivity, and reduced the temporal dependence on the previous year’s growth. These results were significant even 13–14 years after thinning and coherent for the three study sites. Differences were found between the sites regarding the physiological mechanisms of adaptation. In two sites, we inferred the enhanced WUEi was due to increased photosynthetic rates (A) at constant stomatal conductance (gs). In the third site, which had higher tree density and therefore competition, we inferred increases in both A and gs, with the former being proportionally larger than the latter.
Differences in Soil Physicochemical Properties in Different-Aged Pinus massoniana Plantations in Southwest China
Authors: Yin Xiaoai , Zhao Longshan , Fang Qian , Ding Guijie
Published: July 2021

With increasing age, plants will cause changes in soil physicochemical properties. The objective of this study was to investigate differences in the soil physicochemical properties in different-aged Masson pine forest plantations (i.e., 10, 20, 40, and 60 years old). Soil samples were collected in a pure Masson pine forest plantation in Southwest China. The soil determination indexes included organic carbon, nitrogen, phosphorus and potassium contents, water content, bulk density, and pH. The soil pH of a 20-year-old forest was significantly (p < 0.05) higher than that of a 10, 40, and 60-year-old forest. In addition, soil-available phosphorus in a 60-year-old forest was significantly (p < 0.05) higher than that in the other three age forest groups. With increasing forest age, available phosphorus increased, while available nitrogen decreased at 20 years old and then increased at 40 years old. There was a significant positive correlation (p < 0.05) between total nitrogen and available potassium; no significant correlation (p> 0.05) between total phosphorus and total potassium, organic carbon, bulk density, and pH; and a significant negative correlation (p < 0.05) between available phosphorus and the water content. The availability and utilization efficiency of soil nutrients in young forests were higher than those in old forests and the intermediate forest age was an important time point that affected the soil properties. To improve the availability of soil nutrients and ensure the sustainable utilization of soil resources, it is necessary to increase the input of nitrogen and especially phosphorus. More attention should be given to the phytochemometric response with respect to the age of plantations.
Forest Restoration: Do Site Selection and Restoration Practices Follow Ecological Criteria? A Case Study in Sweden
Authors: Hof Anouschka R. , Zachrisson Anna , Polvi Lina E.
Published: July 2021

The speed with which restoration will, or can, be accomplished depends on the initial state and location of the sites. However, many factors can undermine the process of choosing sites that are deemed the best ecological choice for restoration. Little attention has been paid to whether site selection follows ecological criteria and how this may affect restoration success. We used habitat inventory data to investigate whether ecological criteria for site selection and restoration have been followed, focusing on restoration for the white-backed woodpecker (Dendrocopos leucotos B.) in Sweden. In our study region, which is situated in an intensively managed forest landscape with dense and young stands dominated by two coniferous species, purely ecological criteria would entail that sites that are targeted for restoration would (1) initially be composed of older and more deciduous trees than the surrounding landscape, and (2) be at a scale relevant for the species. Furthermore, restoration should lead to sites becoming less dense and less dominated by coniferous trees after restoration, which we investigated as an assessment of restoration progress. To contextualize the results, we interviewed people involved in the restoration efforts on site. We show that although the first criterion for ecological site selection was largely met, the second was not. More research is needed to assess the motivations of actors taking part in restoration efforts, as well as how they interlink with public efforts. This would allow us to identify possible synergies that can benefit restoration efforts.
Implementation of Parallel Cascade Identification at Various Phases for Integrated Navigation System
Authors: Iqbal Umar , Abosekeen Ashraf , Georgy Jacques , Umar Areejah , Noureldin Aboelmagd , Korenberg Michael J.
Published: July 2021

Global navigation satellite systems (GNSS) are widely used for the navigation of land vehicles. However, the positioning accuracy of GNSS, such as the global positioning system (GPS), deteriorates in urban areas due to signal blockage and multipath effects. GNSS can be integrated with a micro-electro-mechanical system (MEMS)–based inertial navigation system (INS), such as a reduced inertial sensor system (RISS) using a Kalman filter (KF) to enhance the performance of the integrated navigation solution in GNSS challenging environments. The linearized KF cannot model the low-cost and small-size sensors due to relatively high noise levels and compound error characteristics. This paper reviews two approaches to employing parallel cascade identification (PCI), a non-linear system identification technique, augmented with KF to enhance the navigational solution. First, PCI models azimuth errors for a loosely coupled 2D RISS integrated system with GNSS to obtain a navigation solution. The experimental results demonstrated that PCI improved the integrated 2D RISS/GNSS performance by modeling linear, non-linear, and other residual azimuth errors. For the second scenario, PCI is utilized for modeling residual pseudorange correlated errors of a KF-based tightly coupled RISS/GNSS navigation solution. Experimental results have shown that PCI enhances the performance of the tightly coupled KF by modeling the non-linear pseudorange errors to provide an enhanced and more reliable solution. For the first algorithm, the results demonstrated that PCI can enhance the performance by 77% as compared to the KF solution during the GNSS outages. For the second algorithm, the performance improvement for the proposed PCI technique during the availability of three satellites was 39% compared to the KF solution.
T-Cell Acute Lymphoblastic Leukemia: Biomarkers and Their Clinical Usefulness
Authors: Bardelli Valentina , Arniani Silvia , Pierini Valentina , Di Giacomo Danika , Pierini Tiziana , Gorello Paolo , Mecucci Cristina , La Starza Roberta
Published: July 2021

T-cell acute lymphoblastic leukemias (T-ALL) are immature lymphoid tumors localizing in the bone marrow, mediastinum, central nervous system, and lymphoid organs. They account for 10–15% of pediatric and about 25% of adult acute lymphoblastic leukemia (ALL) cases. It is a widely heterogeneous disease that is caused by the co-occurrence of multiple genetic abnormalities, which are acquired over time, and once accumulated, lead to full-blown leukemia. Recurrently affected genes deregulate pivotal cell processes, such as cycling (CDKN1B, RB1, TP53), signaling transduction (RAS pathway, IL7R/JAK/STAT, PI3K/AKT), epigenetics (PRC2 members, PHF6), and protein translation (RPL10, CNOT3). A remarkable role is played by NOTCH1 and CDKN2A, as they are altered in more than half of the cases. The activation of the NOTCH1 signaling affects thymocyte specification and development, while CDKN2A haploinsufficiency/inactivation, promotes cell cycle progression. Among recurrently involved oncogenes, a major role is exerted by T-cell-specific transcription factors, whose deregulated expression interferes with normal thymocyte development and causes a stage-specific differentiation arrest. Hence, TAL and/or LMO deregulation is typical of T-ALL with a mature phenotype (sCD3 positive) that of TLX1, NKX2-1, or TLX3, of cortical T-ALL (CD1a positive); HOXA and MEF2C are instead over-expressed in subsets of Early T-cell Precursor (ETP; immature phenotype) and early T-ALL. Among immature T-ALL, genomic alterations, that cause BCL11B transcriptional deregulation, identify a specific genetic subgroup. Although comprehensive cytogenetic and molecular studies have shed light on the genetic background of T-ALL, biomarkers are not currently adopted in the diagnostic workup of T-ALL, and only a limited number of studies have assessed their clinical implications. In this review, we will focus on recurrent T-ALL abnormalities that define specific leukemogenic pathways and on oncogenes/oncosuppressors that can serve as diagnostic biomarkers. Moreover, we will discuss how the complex genomic profile of T-ALL can be used to address and test innovative/targeted therapeutic options.
Expression of a Truncated Yeast Ccc1 Vacuolar Transporter Increases the Accumulation of Endogenous Iron
Authors: Sorribes-Dauden Raquel , Martínez-Pastor María Teresa , Puig Sergi
Published: July 2021

Iron is an essential micronutrient for all eukaryotic organisms because it participates as a redox cofactor in multiple metabolic processes. Iron bioavailability is highly restricted due to the low solubility of its oxidized form, frequently leading to iron deficiency anemia. The baker’s yeast Saccharomyces cerevisiae is used as a model organism for iron homeostasis studies, but also as a food supplement and fermentative microorganism in the food industry. Yeast cells use the vacuolar Ccc1 transporter to detoxify and store excess iron in the vacuoles. Here, we modulate CCC1 expression and properties to increase iron extraction from the environment. We show that constitutive expression of full-length CCC1 is toxic, whereas deletion of its cytosolic amino-terminal (Nt) domain (NtΔCCC1) rescues this phenotype. Toxicity is exacerbated in cells lacking AFT1 transcription factor. Further characterization of NtΔCcc1 protein suggests that it is a partially functional protein. Western blot analyses indicate that deletion of Ccc1 Nt domain does not significantly alter GFP-Ccc1 protein stability. A functional full-length GFP-Ccc1 protein localized to particular regions of the vacuolar membrane, whereas GFP-NtΔCcc1 protein was evenly distributed throughout this endogenous membrane. Interestingly, expression of NtΔCCC1 increased the accumulation of endogenous iron in cells cultivated under iron-sufficient conditions, a strategy that could be used to extract iron from media that are not rich in iron.
The Chloroplast Epitranscriptome: Factors, Sites, Regulation, and Detection Methods
Authors: Manavski Nikolay , Vicente Alexandre , Chi Wei , Meurer Jörg
Published: July 2021

Modifications in nucleic acids are present in all three domains of life. More than 170 distinct chemical modifications have been reported in cellular RNAs to date. Collectively termed as epitranscriptome, these RNA modifications are often dynamic and involve distinct regulatory proteins that install, remove, and interpret these marks in a site-specific manner. Covalent nucleotide modifications-such as methylations at diverse positions in the bases, polyuridylation, and pseudouridylation and many others impact various events in the lifecycle of an RNA such as folding, localization, processing, stability, ribosome assembly, and translational processes and are thus crucial regulators of the RNA metabolism. In plants, the nuclear/cytoplasmic epitranscriptome plays important roles in a wide range of biological processes, such as organ development, viral infection, and physiological means. Notably, recent transcriptome-wide analyses have also revealed novel dynamic modifications not only in plant nuclear/cytoplasmic RNAs related to photosynthesis but especially in chloroplast mRNAs, suggesting important and hitherto undefined regulatory steps in plastid functions and gene expression. Here we report on the latest findings of known plastid RNA modifications and highlight their relevance for the post-transcriptional regulation of chloroplast gene expression and their role in controlling plant development, stress reactions, and acclimation processes.
Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale
Authors: Jian Yue , Zhang Chenlu , Wang Yating , Li Zhiqing , Chen Jing , Zhou Wenting , Huang Wenli , Jiang Min , Zheng Hao , Li Mengyao , Miao Huiying , Zhang Fen , Li Huanxiu , Wang Qiaomei , Sun Bo
Published: July 2021

Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was cloned from the white flower cultivar of Chinese kale and was expressed in all developmental stages and organs of Chinese kale; its expression was highest in young seeds. The subcellular localization indicated that BoaNXS was localized in the chloroplast. BoaNXS-overexpressed plants were obtained via Agrobacterium-mediated transient overexpression methodology, and the gene overexpression efficiencies ranged from 2.10- to 4.24-fold. The color in the leaves of BoaNXS-overexpressed plants changed from green to yellow-green; the content of total and individual carotenoids, such as neoxanthin, violaxanthin, and lutein, was significantly increased, and the expression levels of most carotenoid biosynthetic genes were notably increased. These findings indicated that BoaNXS is of vital importance in carotenoid biosynthesis in Chinese kale and could be used as a candidate gene for enriching the carotenoid accumulation and color of Chinese kale and other Brassica vegetables.
Advances with Long Non-Coding RNAs in Alzheimer’s Disease as Peripheral Biomarker
Authors: Garofalo Maria , Pandini Cecilia , Sproviero Daisy , Pansarasa Orietta , Cereda Cristina , Gagliardi Stella
Published: July 2021

One of the most compelling needs in the study of Alzheimer’s disease (AD) is the characterization of cognitive decline peripheral biomarkers. In this context, the theme of altered RNA processing has emerged as a contributing factor to AD. In particular, the significant role of long non-coding RNAs (lncRNAs) associated to AD is opening new perspectives in AD research. This class of RNAs may offer numerous starting points for new investigations about pathogenic mechanisms and, in particular, about peripheral biomarkers. Indeed, altered lncRNA signatures are emerging as potential diagnostic biomarkers. In this review, we have collected and fully explored all the presented data about lncRNAs and AD in the peripheral system to offer an overview about this class of non-coding RNAs and their possible role in AD.
RNA Modifications in Pathogenic Bacteria: Impact on Host Adaptation and Virulence
Authors: Antoine Laura , Bahena-Ceron Roberto , Devi Bunwaree Heemee , Gobry Martin , Loegler Victor , Romby Pascale , Marzi Stefano
Published: July 2021

RNA modifications are involved in numerous biological processes and are present in all RNA classes. These modifications can be constitutive or modulated in response to adaptive processes. RNA modifications play multiple functions since they can impact RNA base-pairings, recognition by proteins, decoding, as well as RNA structure and stability. However, their roles in stress, environmental adaptation and during infections caused by pathogenic bacteria have just started to be appreciated. With the development of modern technologies in mass spectrometry and deep sequencing, recent examples of modifications regulating host-pathogen interactions have been demonstrated. They show how RNA modifications can regulate immune responses, antibiotic resistance, expression of virulence genes, and bacterial persistence. Here, we illustrate some of these findings, and highlight the strategies used to characterize RNA modifications, and their potential for new therapeutic applications.
Allelic Variants in Established Hypopituitarism Genes Expand Our Knowledge of the Phenotypic Spectrum
Authors: Nakaguma Marilena , Ferreira Nathalia Garcia Bianchi Pereira , Benedetti Anna Flavia Figueredo , Madi Mariana Cotarelli , Silva Juliana Moreira , Li Jun Z. , Ma Qianyi , Bilge Ozel Ayse , Fang Qing , Narcizo Amanda de Moraes , Cardoso Laís Cavalca , Montenegro Luciana Ribeiro , Funari Mariana Ferreira de Assis , Nishi Mirian Yumie , Arnhold Ivo Jorge Prado , Jorge Alexander Augusto de Lima , Mendonca Berenice Bilharinho de , Camper Sally Ann , Carvalho Luciani R.
Published: July 2021

We report four allelic variants (three novel) in three genes previously established as causal for hypopituitarism or related disorders. A novel homozygous variant in the growth hormone gene, GH1 c.171delT (p.Phe 57Leufs*43), was found in a male patient with severe isolated growth hormone deficiency (IGHD) born to consanguineous parents. A hemizygous SOX3 allelic variant (p.Met304Ile) was found in a male patient with IGHD and hypoplastic anterior pituitary. YASARA, a tool to evaluate protein stability, suggests that p.Met304Ile destabilizes the SOX3 protein (ΔΔG = 2.49 kcal/mol). A rare, heterozygous missense variant in the TALE homeobox protein gene, TGIF1 (c.268C>T:p.Arg90Cys) was found in a patient with combined pituitary hormone deficiency (CPHD), diabetes insipidus, and syndromic features of holoprosencephaly (HPE). This variant was previously reported in a patient with severe holoprosencephaly and shown to affect TGIF1 function. A novel heterozygous TGIF1 variant (c.82T>C:p.Ser28Pro) was identified in a patient with CPHD, pituitary aplasia and ectopic posterior lobe. Both TGIF1 variants have an autosomal dominant pattern of inheritance with incomplete penetrance. In conclusion, we have found allelic variants in three genes in hypopituitarism patients. We discuss these variants and associated patient phenotypes in relation to previously reported variants in these genes, expanding our knowledge of the phenotypic spectrum in patient populations.
Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma
Authors: Rothzerg Emel , Ho Xuan Dung , Xu Jiake , Wood David , Märtson Aare , Kõks Sulev
Published: July 2021

The human genome encodes thousands of natural antisense long noncoding RNAs (lncRNAs); they play the essential role in regulation of gene expression at multiple levels, including replication, transcription and translation. Dysregulation of antisense lncRNAs plays indispensable roles in numerous biological progress, such as tumour progression, metastasis and resistance to therapeutic agents. To date, there have been several studies analysing antisense lncRNAs expression profiles in cancer, but not enough to highlight the complexity of the disease. In this study, we investigated the expression patterns of antisense lncRNAs from osteosarcoma and healthy bone samples (24 tumour-16 bone samples) using RNA sequencing. We identified 15 antisense lncRNAs (RUSC1-AS1, TBX2-AS1, PTOV1-AS1, UBE2D3-AS1, ERCC8-AS1, ZMIZ1-AS1, RNF144A-AS1, RDH10-AS1, TRG-AS1, GSN-AS1, HMGA2-AS1, ZNF528-AS1, OTUD6B-AS1, COX10-AS1 and SLC16A1-AS1) that were upregulated in tumour samples compared to bone sample controls. Further, we performed real-time polymerase chain reaction (RT-qPCR) to validate the expressions of the antisense lncRNAs in 8 different osteosarcoma cell lines (SaOS-2, G-292, HOS, U2-OS, 143B, SJSA-1, MG-63, and MNNG/HOS) compared to hFOB (human osteoblast cell line). These differentially expressed IncRNAs can be considered biomarkers and potential therapeutic targets for osteosarcoma.
Gender Differences in Depression and Sex Hormones among Patients Receiving Long-Term Opioid Treatment for Chronic Noncancer Pain in Taiwan—A Multicenter Cross-Sectional Study
Authors: Ho Shung-Tai , Lin Tso-Chou , Yeh Chun-Chang , Cheng Kuang-I , Sun Wei-Zen , Sung Chun-Sung , Wen Yeong-Ray , Hsieh Yi-Jer , Wang Po-Kai , Liu Yen-Chin , Tsai Yu-Chuan
Published: July 2021

Background: Long-term use of opioids for chronic noncancer pain is associated with sex hormone disturbances. The interferences with sex hormones, sexual function, and depression were investigated in patients with chronic noncancer pain. Methods: A cross-sectional multicenter survey was conducted on 170 officially registered outpatients receiving long-term opioid treatment in nine medical centers in Taiwan between October 2018 and July 2019. Serum sex hormone levels were examined after the collection of self-administered questionnaires containing the Taiwanese version of the Brief Pain Inventory, depressive status, and sexual function interference. Results: Among 117 (68.8%) questionnaire responses from 170 enrolled outpatients, 38 women and 62 men completed the sex hormone tests, among whom only 23 (23%) had previously received blood hormone tests. Low serum total testosterone levels were detected in 34 (89.5%) women (<30 ng/dL) and 31 (50%) men (<300 ng/dL). Over 60% of women and men reported reduced sexual desire and function despite a nearly 50% reduction in pain intensity and daily function interference over the previous week after opioid treatment. Women generally had higher risks of a depression diagnosis (p = 0.034) and severe depressive symptoms (p = 0.003) and nonsignificantly lower opioid treatment duration (median 81 vs. 120 months) and morphine milligram equivalent (median 134 vs. 165 mg/day) compared with men. Conclusions: This survey demonstrated the high prevalence of depression diagnosis, low sex hormone levels, and reduced sexual function among Taiwanese patients with chronic noncancer pain receiving prolonged opioid therapy. Regular hypogonadal screenings are recommended for further management.
Validation and Psychometric Properties of the Spanish Version of the Hopkins Symptom Checklist-25 Scale for Depression Detection in Primary Care
Authors: Rodríguez-Barragán María , Fernández-San-Martín María Isabel , Clavería-Fontán Ana , Aldecoa-Landesa Susana , Casajuana-Closas Marc , Llobera Joan , Oliván-Blázquez Bárbara , Peguero-Rodríguez Eva
Published: July 2021

Depression constitutes a major public health problem due to its high prevalence and difficulty in diagnosis. The Hopkins Symptom Checklist-25 (HSCL-25) scale has been identified as valid, reproducible, effective, and easy to use in primary care (PC). The purpose of the study was to assess the psychometric properties of the HSCL-25 and validate its Spanish version. A multicenter cross-sectional study was carried out at six PC centers in Spain. Validity and reliability were assessed against the structured Composite International Diagnostic Interview (CIDI). Out of the 790 patients, 769 completed the HSCL-25; 738 answered all the items. Global Cronbach’s alpha was 0.92 (0.88 as calculated for the depression dimension and 0.83 for the anxiety one). Confirmatory factor analysis (CFA) showed one global factor and two correlated factors with a correlation of 0.84. Area under the curve (AUC) was 0.89 (CI 95%, 0.86–0.93%). For a 1.75 cutoff point, sensibility was 88.1% (CI 95%, 77.1–95.1%) and specificity was 76.7% (CI 95%, 73.3–79.8%). The Spanish version of the HSCL-25 has a high response percentage, validity, and reliability and is well-accepted by PC patients.
Conspiracy Theories, Psychological Distress, and Sympathy for Violent Radicalization in Young Adults during the COVID-19 Pandemic: A Cross-Sectional Study
Authors: Levinsson Anna , Miconi Diana , Li Zhiyin , Frounfelker Rochelle L. , Rousseau Cécile
Published: July 2021

The COVID-19 pandemic has spread uncertainty, promoted psychological distress, and fueled interpersonal conflict. The concomitant upsurge in endorsement of COVID-19 conspiracy theories is worrisome because they are associated with both non-adherence to public health guidelines and intention to commit violence. This study investigates associations between endorsement of COVID-19 conspiracy theories, support for violent radicalization (VR) and psychological distress among young adults in Canada. We hypothesized that (a) endorsement of COVID-19 conspiracy theories is positively associated with support for VR, and (b) psychological distress modifies the relationship between COVID-19 conspiracy theories and support for VR. A total of 6003 participants aged 18–35 years old residing in four major Canadian cities completed an online survey between 16 October 2020 and 17 November 2020, that included questions about endorsement of COVID-19 conspiracy theories, support for VR, psychological distress, and socio-economic status. Endorsement of conspiracy theories was associated with support for VR in multivariate regression (β = 0.88, 95% confidence interval (CI) 0.80–0.96). There is a significant interaction effect between endorsement of COVID-19 conspiracy theories and psychological distress (β = 0.49, 95% CI 0.40–0.57). The magnitude of the association was stronger in individuals reporting high psychological distress (β = 1.36, 95% CI 1.26–1.46) compared to those reporting low psychological distress (β = 0.47, 95% CI 0.35–0.59). The association between endorsement of COVID-19 conspiracy theories and VR represents a public health challenge requiring immediate attention. The interaction with psychological distress suggests that policy efforts should combine communication and psychological strategies to mitigate the legitimation of violence.
COVID-19 Prevention and Control Measures in Workplace Settings: A Rapid Review and Meta-Analysis
Authors: Ingram Carolyn , Downey Vicky , Roe Mark , Chen Yanbing , Archibald Mary , Kallas Kadri-Ann , Kumar Jaspal , Naughton Peter , Uteh Cyril Onwuelazu , Rojas-Chaves Alejandro , Shrestha Shibu , Syed Shiraz , Cléirigh Büttner Fionn , Buggy Conor , Perrotta Carla
Published: July 2021

Workplaces can be high-risk environments for SARS-CoV-2 outbreaks and subsequent community transmission. Identifying, understanding, and implementing effective workplace SARS-CoV-2 infection prevention and control (IPC) measures is critical to protect workers, their families, and communities. A rapid review and meta-analysis were conducted to synthesize evidence assessing the effectiveness of COVID-19 IPC measures implemented in global workplace settings through April 2021. Medline, Embase, PubMed, and Cochrane Library were searched for studies that quantitatively assessed the effectiveness of workplace COVID-19 IPC measures. The included studies comprised varying empirical designs and occupational settings. Measures of interest included surveillance measures, outbreak investigations, environmental adjustments, personal protective equipment (PPE), changes in work arrangements, and worker education. Sixty-one studies from healthcare, nursing home, meatpacking, manufacturing, and office settings were included, accounting for ~280,000 employees based in Europe, Asia, and North America. Meta-analyses showed that combined IPC measures resulted in lower employee COVID-19 positivity rates (0.2% positivity; 95% CI 0–0.4%) than single measures such as asymptomatic PCR testing (1.7%; 95% CI 0.9–2.9%) and universal masking (24%; 95% CI 3.4–55.5%). Modelling studies showed that combinations of (i) timely and widespread contact tracing and case isolation, (ii) facilitating smaller worker cohorts, and (iii) effective use of PPE can reduce workplace transmission. Comprehensive COVID-19 IPC measures incorporating swift contact tracing and case isolation, PPE, and facility zoning can effectively prevent workplace outbreaks. Masking alone should not be considered sufficient protection from SARS-CoV-2 outbreaks in the workplace.
Research on the Performance of Human Capital at Different Organizational Levels of Pharmaceutical Corporations: Moderation of Informal Relational Capital
Authors: Pi Shenglei , Chang Kuei-Feng , Shih I-Tung
Published: July 2021

As intellectual capital is considered an important strategic resource in knowledge-intensive industries, such as the health and pharmaceutical industries, scholars have developed a deeper understanding of the human capital at different levels of organizations and its interaction with relational capital from informal institutional stakeholders. This study focused on the role of human capital at different levels of pharmaceutical corporations and the orchestration of human capital at different levels with informal relational capital. By using data regarding Chinese pharmaceutical listed companies from 2001 to 2017, this study found that (1) human capital at the employee level exerted an inverted U-shaped effect on a pharmaceutical firm’s performance, which was negatively moderated by informal institutional relational capital, and (2) based on the upper echelons theory, human capital at the executive level had a monotonic positive impact on a pharmaceutical firm’s performance but was negatively moderated by informal relational capital. We found two arrangements that facilitate the orchestration of intellectual capital components to gain optimal distinctiveness and performance.
Effect of Resonant Frequency Vibration on Delayed Onset Muscle Soreness and Resulting Stiffness as Measured by Shear-Wave Elastography
Authors: Jones Garrett C. , Blotter Jonathan D. , Smallwood Cameron D. , Eggett Dennis L. , Cochrane Darryl J. , Feland J. Brent
Published: July 2021

This study utilized resonant frequency vibration to the upper body to determine changes in pain, stiffness and isometric strength of the biceps brachii after eccentric damage. Thirty-one participants without recent resistance training were randomized into three groups: a Control (C) group and two eccentric exercise groups (No vibration (NV) and Vibration (V)). After muscle damage, participants in the V group received upper body vibration (UBV) therapy for 5 min on days 1–4. All participants completed a visual analog scale (VAS), maximum voluntary isometric contraction (MVIC), and shear wave elastography (SWE) of the bicep at baseline (pre-exercise), 24 h, 48 h, and 1-week post exercise. There was a significant difference between V and NV at 24 h for VAS (p = 0.0051), at 24 h and 1-week for MVIC (p = 0.0017 and p = 0.0016, respectively). There was a significant decrease in SWE for the V group from 24–48 h (p = 0.0003), while there was no significant change in the NV group (p = 0.9341). The use of UBV resonant vibration decreased MVIC decrement and reduced VAS pain ratings at 24 h post eccentric damage. SWE was strongly negatively correlated with MVIC and may function as a predictor of intrinsic muscle state in the time course of recovery of the biceps brachii.
Science Policy to Advance a Climate Change and Health Research Agenda in the United States
Authors: Madrigano Jaime , Shih Regina A. , Izenberg Maxwell , Fischbach Jordan R. , Preston Benjamin L.
Published: July 2021

Climate change is thought to be one of the greatest public health threats of the 21st century and there has been a tremendous growth in the published literature describing the health implications of climate change over the last decade. Yet, there remain several critical knowledge gaps in this field. Closing these gaps is crucial to developing effective interventions to minimize the health risks from climate change. In this commentary, we discuss policy trends that have influenced the advancement of climate change and health research in the United States context. We then enumerate specific knowledge gaps that could be addressed by policies to advance scientific research. Finally, we describe tools and methods that have not yet been fully integrated into the field, but hold promise for advancing the science. Prioritizing this advancement offers the potential to improve public health-related policies on climate change.
From Exposure to Violence between Mother and Her Intimate Partner to Suicidality Experienced by Urban Adolescents in Chicago’s Southside
Authors: Hong Jun Sung , Zhang Saijun , Burlaka Viktor , Yoshihama Mieko , Yan Yueqi , Voisin Dexter R.
Published: July 2021

Although the relationship between exposure to intimate partner violence and youths’ psychological and other wellbeing has been widely studied, there is limited research about how youths’ exposure to violence between mother and her intimate partner may be related to youth psychological wellbeing. The study used a sample of urban adolescents in Chicago Southbound to examine whether youths’ exposure to verbal conflict between mother and her intimate partner is related to their suicidality and whether youth depression and aggression may be in between such a linkage. Our findings indicated that one-third of the youth had suicidal thoughts or suicidal/self-hurting attempts. Youths’ exposure to verbal conflict between mother and her intimate partner was associated with their depressive and aggressive symptoms, and depressive symptoms subsequently were linked to suicidality. The findings also showed that youth depressive symptoms and aggressive symptoms were positively correlated, which may influence their associations with suicidality. We concluded that youth exposure to parental intimate partner violence, even comparatively mild forms such as a verbal conflict between mother and her intimate partner, may increase their risk of suicidality by worsening psychological wellbeing. The findings highlight the importance of tackling youth suicidality risks while accounting for their exposure to intimate partner violence including verbal conflicts between parents.
Effects of a Midwife-Coordinated Maternity Care Intervention (ChroPreg) vs. Standard Care in Pregnant Women with Chronic Medical Conditions: Results from a Randomized Controlled Trial
Authors: de Wolff Mie G. , Midtgaard Julie , Johansen Marianne , Rom Ane L. , Rosthøj Susanne , Tabor Ann , Hegaard Hanne K.
Published: July 2021

The proportion of childbearing women with pre-existing chronic medical conditions (CMC) is rising. In a randomized controlled trial, we aimed to evaluate the effects of a midwife-coordinated maternity care intervention (ChroPreg) in pregnant women with CMC. The intervention consisted of three main components: (1) Midwife-coordinated and individualized care, (2) Additional ante-and postpartum consultations, and (3) Specialized known midwives. The primary outcome was the total length of hospital stay (LOS). Secondary outcomes were patient-reported outcomes measuring psychological well-being and satisfaction with maternity care, health utilization, and maternal and infant outcomes. A total of 362 women were randomized to the ChroPreg intervention (n = 131) or Standard Care (n = 131). No differences in LOS were found between groups (median 3.0 days, ChroPreg group 0.1% lower LOS, 95% CI −7.8 to 7%, p = 0.97). Women in the ChroPreg group reported being more satisfied with maternity care measured by the Pregnancy and Childbirth Questionnaire (PCQ) compared with the Standard Care group (mean PCQ 104.5 vs. 98.2, mean difference 6.3, 95% CI 3.0–10.0, p < 0.0001). In conclusion, the ChroPreg intervention did not reduce LOS. However, women in the ChroPreg group were more satisfied with maternity care.
Deep Venous Thrombosis and Risk of Consequent Sepsis Event: A Retrospective Nationwide Population-Based Cohort Study
Authors: Yeh Ying-Tung , Tsai Sheng-En , Chen Ying-Cheng , Yang Shun-Fa , Yeh Han-Wei , Wang Bo-Yuan , Yeh Liang-Tsai , Shih Nai-Chen , Wang Yu-Hsun , Chen Yin-Yang , Yeh Chao-Bin
Published: July 2021

Deep vein thrombosis causes several acute and chronic vessel complications and puts patients at risk of subsequent sepsis development. This unique study aimed to estimate the risk of sepsis development in DVT patients compared with non-DVT patients. This population-based cohort study used records of a longitudinal health insurance database containing two million patients defined in Taiwan’s National Health Insurance Research Database (NHIRD). Our study included patients aged over 20 years with a new diagnosis of DVT with at least two outpatient department visits or an admission between 2001 and 2014. Patients with a diagnosis of sepsis before the index date were excluded. Propensity score matching (PSM) was used to homogenize the baseline characteristics between the two groups. To define the independent risk of the DVT group, a multivariate Cox proportional hazard model was used to estimate the hazard ratios. After PSM, the DVT group (n = 5753) exhibited a higher risk of sepsis (adjusted hazard ratio, aHR, 1.74; 95% CI, 1.59–1.90) compared with non-DVT group (n = 5753). Patients with an increased risk of sepsis were associated with being elderly aged, male, having diabetes, chronic kidney disease, chronic obstructive pulmonary disease, stroke, malignancy, and use of antibiotics. In conclusion, this population-based cohort study demonstrated an increased risk of sepsis in DVT patients compared with non-DVT patients. Thus, early prevention and adequate treatment of DVT is necessary in clinical practice.
Pace Controlled by a Steady-State Physiological Variable Is Associated with Better Performance in a 3000 M Run
Authors: Molinari Claire A. , Bresson Pierre , Palacin Florent , Billat Véronique
Published: July 2021

This paper aims to test the hypothesis whereby freely chosen running pace is less effective than pace controlled by a steady-state physiological variable. Methods Eight runners performed four maximum-effort 3000 m time trials on a running track. The first time trial (TT1) was freely paced. In the following 3000 m time trials, the pace was controlled so that the average speed (TT2), average V˙O2 (TT3) or average HR (TT4) recorded in TT1 was maintained throughout the time trial. Results: Physiologically controlled pace was associated with a faster time (mean ± standard deviation: 740 ± 34 s for TT3 and 748 ± 33 s for TT4, vs. 854 ± 53 s for TT1; p < 0.01), a lower oxygen cost of running (200 ± 5 and 220 ± 3 vs. 310 ± 5 mLO2·kg−1·km−1, respectively; p < 0.02), a lower cardiac cost (0.69 ± 0.08 and 0.69 ± 0.04 vs. 0.86 ± 0.09 beat·m−1, respectively; p < 0.01), and a more positively skewed speed distribution (skewness: 1.7 ± 0.9 and 1.3 ± 0.6 vs. 0.2 ± 0.4, p < 0.05). Conclusion: Physiologically controlled pace (at the average V˙O2 or HR recorded in a freely paced run) was associated with a faster time, a more favorable speed distribution and lower levels of physiological strain, relative to freely chosen pace. This finding suggests that non-elite runners do not spontaneously choose the best pace strategy.