bluuebunny/crossref_metadata_2025 huggingface dataset:
Concepts on Train-to-Ground Wireless Communication System for Hyperloop: Channel, Network Architecture, and Resource Management
Authors: Zhang Jiachi , Liu Liu , Han Botao , Li Zheng , Zhou Tao , Wang Kai , Wang Dong , Ai Bo
Published: August 2020

Hyperloop is envisioned as a novel transportation way with merits of ultra-high velocity and great traveling comforts. In this paper, we present some concepts on the key technologies dedicated to the train-to-ground communication system based on some prevailing fifth-generation communication (5G) technologies from three aspects: wireless channel, network architecture, and resource management. First, we characterize the wireless channel of the distributed antenna system (DAS) using the propagation-graph channel modelling theory. Simulation reveals that a drastic Doppler shift variation appears when crossing the trackside antenna. Hence, the leaky waveguide system is a promising way to provide a stable receiving signal. In this regard, the radio coverage is briefly estimated. Second, a cloud architecture is utilized to integrate several successive trackside leaky waveguides into a logical cell to reduce the handover frequency. Moreover, based on a many-to-many mapping relationship between distributed units (DUs) and centralized units (CUs), a novel access network architecture is proposed to reduce the inevitable handover cost by using the graph theory. Simulation results show that this scheme can yield a low handover cost. Then, with regards to the ultra-reliable and low latency communication (uRLLC) traffic, a physical resource block (PRB) multiplexing scheme considering the latency requirements of each traffic type is exploited. Simulation presents that this scheme can maximize the throughput of non-critical mission communication services while guaranteeing the requirements of uRLLC traffic. Finally, in terms of the non-critical mission communication services, two cache-based resource management strategies are proposed to boost the throughput and reduce the midhaul link burden by pre-fetching and post-uploading schemes. Simulation demonstrates that the cache-based schemes can boost the throughput dramatically.
Mathematical Model of Thermodynamic Processes in the Intake Manifold of a Diesel Engine with Fuel and Water Injection
Authors: Bondar Vladimir , Aliukov Sergei , Malozemov Andrey , Das Arkaprava
Published: August 2020

The article presents the results of a study aimed at creating a mathematical model of thermodynamic processes in the intake manifold of a forced diesel engine, taking into account the features of simultaneous injection of fuel and water into the collector. In the course of the study, the tasks of developing a mathematical model were solved, it was implemented in the existing software for component simulation “Internal combustion engine research and development” (ICE RnD), created using the Modelica language, and verification was undertaken using the results of bench tests of diesel engines with injection fuel and water into the intake manifold. The mathematical model is based on a system of equations for the energy and mass balances of gases and includes detailed mathematical submodels of the processes of simultaneous evaporation of fuel and water in the intake manifold; it takes into account the effect of the evaporation of fuel and water on the parameters of the gas state in the intake manifold; it takes into account the influence of the state parameters of the working fluid in the intake manifold on the physical characteristics of fuel and water; it meets the principles of component modeling, since it does not contain parameters that are not related to the simulated component; it describes the process of simultaneous transfer of vapors and non-evaporated liquids between components; and it does not include empirical relationships requiring data on the dynamics of fuel evaporation under reference conditions. According to the results of a full-scale experiment, the adequacy of the mathematical model developed was confirmed. This model can be used to determine the rational design parameters of the fuel and water injection system, the adjusting parameters of the forced diesel engine that provide the required power, and economic indicators, taking into account the limitations on the magnitude of the mechanical and thermal loads of its parts.
Post-Modern Epidemiology: Back to the Populations
Authors: Chiolero Arnaud
Published: August 2020

The creation of new journal about epidemiology is a good opportunity to think about the state of the field and to make proposals for its development [...]
Patterns for Populus spp. Stand Biomass in Gradients of Winter Temperature and Precipitation of Eurasia
Authors: Usoltsev Vladimir Andreevich , Chen Baozhang , Shobairi Seyed Omid Reza , Tsepordey Ivan Stepanovich , Chasovskikh Viktor Petrovich , Anees Shoaib Ahmad
Published: August 2020

Based on a generated database of 413 sample plots, with definitions of stand biomass of the genus Populus spp. in Eurasia, from France to Japan and southern China, statistically significant changes in the structure of forest stand biomass were found, with shifts in winter temperatures and average annual precipitation. When analyzing the reaction of the structure of the biomass of the genus Populus to temperature and precipitation in their transcontinental gradients, a clearly expressed positive relationship of all components of the biomass with the temperature in January is visible. Their relationship with precipitation is less clear; in warm climate zones, when precipitation increases, the biomass of all wood components decreases intensively, and in cold climate zones, this decrease is less pronounced. The foliage biomass does not increase when precipitation decreases, as is typical for wood components, but decreases. This can be explained by the specifics of the functioning of the assimilation apparatus, namely its transpiration activity when warming, and the corresponding increase in transpiration, which requires an increase in the influx of assimilates into the foliage, and the desiccation of the climate that reduces this influx of assimilates. Comparison of the obtained patterns with previously published results for other species from Eurasia showed partial or complete discrepancies, the causes of which require special physiological studies. The results obtained can be useful in the management of biosphere functions of forests, which is important in the implementation of climate stabilization measures, as well as in the validation of the results of simulation experiments to assess the carbon-deposition capacity of forests.
Chemical, Biological and Mechanical Characterization of Wood Treated with Propolis Extract and Silicon Compounds
Authors: Woźniak Magdalena , Kwaśniewska-Sip Patrycja , Krueger Michał , Roszyk Edward , Ratajczak Izabela
Published: August 2020

The development of new bio-friendly alternatives for wood conservation is of great interest and necessary for environmental protection. In this paper, the preparations based on the propolis extract and silicon compounds were used as green wood preservatives. The wood was treated with 15% propolis extract (EEP) and two propolis-silane preparations, namely, EEP-VTMOS/TEOS (EEP with vinyltrimethoxysilane and tetraethyl orthosilicate) and EEP-MPTMOS/TEOS (EEP with 3-(trimethoxysilyl) propyl methacrylate and tetraethyl orthosilicate). The aim of the research was to determine the properties of treated wood, which was characterized by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), atomic absorption spectroscopy (AAS), X-ray fluorescence (XRF), and scanning electron microscopy (SEM). Moreover, the resistance against brown-rot fungus Coniophora puteana and the mechanical properties of treated wood were also determined. The analysis of phenolic compounds concentration in treated wood indicated that phenols were in greater extent leached from wood treated with the propolis extract than from wood impregnated with the propolis-silane preparations. The presence of silicon in treated wood both before and after leaching was confirmed by CP MAS NMR measurements. In turn, AAS and XRF analyses indicated that the degree of Si leaching from wood impregnated with EEP-VTMOS/TEOS was approximately two times lower than from EEP-MPTMOS/TEOS treated wood. The results of chemical analyses confirmed that the constituents of the propolis-silane preparations formed permanent bonds with wood. In turn, the results of the antifungal efficacy against C. puteana showed that the propolis extract and the propolis-silane preparations limited the fungus activity, even the wood was subjected to leaching procedure. The treated wood showed an increase in bending strength and a decrease in the modulus of elasticity compared to untreated wood. The obtained results indicate that the propolis-silane preparations can be promising green wood preservatives, harmless for the natural environment.
Transcriptome Analysis of Bursaphelenchus xylophilus Uncovers the Impact of Stenotrophomonas maltophilia on Nematode and Pine Wilt Disease
Authors: Xue Qi , Wu Xiao-Qin , Wu Fei , Ye Jian-Ren
Published: August 2020

Stenotrophomonas maltophilia influences the reproduction, pathogenicity, and gene expression of aseptic Bursaphelenchus xylophilus after inoculation of aseptic Pinus massoniana. Pine wilt disease is a destructive pine forest disease caused by B. xylophilus, and its pathogenesis is unclear. The role of bacteria associated with B. xylophilus in pine wilt disease has attracted widespread attention. S. maltophilia is one of the most dominant bacteria in B. xylophilus, and its effect is ambiguous. This study aims to explore the role of S. maltophilia in pine wilt disease. The reproduction and virulence of aseptic B. xylophilus and B. xylophilus containing S. maltophilia were examined by inoculating aseptic P. massoniana seedlings. The gene expressions of two nematode treatments were identified by transcriptome sequencing. The reproduction and virulence of B. xylophilus containing S. maltophilia were stronger than that of aseptic nematodes. There were 4240 differentially expressed genes between aseptic B. xylophilus and B. xylophilus containing S. maltophilia after inoculation of aseptic P. massoniana, including 1147 upregulated genes and 2763 downregulated genes. These differentially expressed genes were significantly enriched in some immune-related gene ontology (GO) categories, such as membrane, transporter activity, metabolic processes, and many immune-related pathways, such as the wnt, rap1, PI3K-Akt, cAMP, cGMP-PKG, MAPK, ECM-receptor interaction, and calcium signaling pathways. The polyubiquitin-rich gene, leucine-rich repeat serine/threonine-protein kinase gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, acetyl-CoA carboxylase gene, and heat shock protein genes were the key genes associated with immune resistance. Moreover, there were four cell wall hydrolase genes, thirty-six detoxification- and pathogenesis-related protein genes, one effector gene and ten cathepsin L-like cysteine proteinase genes that were differentially expressed. After inoculation of the host pine, S. maltophilia could affect the virulence and reproduction of B. xylophilus by regulating the expression of parasitic, immune, and pathogenicity genes of B. xylophilus.
Relationship of Forest Cover Fragmentation and Drought with the Occurrence of Forest Fires in the Department of Santa Cruz, Bolivia
Authors: Maillard Oswaldo , Vides-Almonacid Roberto , Flores-Valencia Marcio , Coronado Roger , Vogt Peter , Vicente-Serrano Sergio M. , Azurduy Huáscar , Anívarro Ruth , Cuellar Rosa Leny
Published: August 2020

The forest fires of 2019 were among the most devastating ever recorded in Bolivia. In this study we analyze the relationship between forest fragmentation and meteorological drought with the spatial distribution of forest fires during that year in the Department of Santa Cruz, Bolivia. We carried out a classification of the natural vegetation using Landsat 8 satellite imagery. Forest fragmentation was defined according to the distribution of forest patch sizes and classified using seven categories; furthermore, distance to anthropogenically used areas and forest edges was quantified. Spatial patterns of meteorological drought severity were quantified using long-term series of precipitation and reference evapotranspiration. Areas burned during 2019 (July–December) were characterized by means of spectral indices (normalized burn ratio (NBR) and normalized delta burn ratio (dNBR)) and unsupervised classification methods (interactive self-organizing data analysis algorithm (ISODATA)). The results show that 61.9% of the total area burned occurred in large (>2,000,000 ha), relatively unfragmented patches. However, the highest proportion of fires (17.1%) occurred in relatively small patches (<20 ha). In addition, anthropogenically used zones and forest edges were most impacted by forest fires. Finally, the spatial patterns of drought severity also influenced the severity of forest fires.
Cinara splendens (Hemiptera: Aphididae: Lachninae)—First Record in Palaearctic Region
Authors: Havelka Jan , Havelka Jekaterina , Starý Petr
Published: August 2020

Nearctic aphid Cinara splendens (Gillette and Palmer, 1924) was collected on ornamental Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) in South Bohemia in 2009. It was the first record of this species in the Palaearctic region. The aim of this research was to study the bionomy of this species in Central Europe and to make descriptions of all available morphs, as previous morphological descriptions of C. splendens appeared to be incomplete. Six monitoring sites of this species were established in South Bohemia and were then regularly attended in the period of 2009–2019. The colonies of C. splendens were observed; its natural enemies and honeydew users were also registered. Aphids were collected for the microscope slide preparation, followed by the evaluation of thirty of the basic quantitative and seven qualitative morphological characteristics. Partial sequences of mitochondrial COI and nuclear EF-1α were used to confirm morphology-based identification and to compare samples from the Czech Republic with those of North American origin. Cinarasplendens survived successfully under new ecological conditions, but its population density remained quite low, except for 2009 and 2019, due to a synergistic effect of the dry weather and very high population density of the adelgid Gilletteella coweni (Gillette, 1907), which is a key pest of Douglas fir in the Czech Republic. The principle predators were coccinellid beetles, while the aphidophagous hover flies were less abundant. Together with a weak ability to migrate due to a low number of alate viviparous females in population, C. splendens cannot be a potential pest of P. menziesii in Central Europe.
Key Techniques for Somatic Embryogenesis and Plant Regeneration of Pinus koraiensis
Authors: Gao Fang , Peng Chunxue , Wang Hao , Tretyakova Iraida Nikolaevna , Nosov Alexander Mikhaylovich , Shen Hailong , Yang Ling
Published: August 2020

Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 μmol·L−1 2,4-D and 2.2 μmol·L−1 6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71·g−1 fresh weight). Adding 1–1.5g L−1 L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63·g−1 fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry.
Side-by-Side Comparison of Clean and Biomass-Derived, Impurity-Containing Syngas as Substrate for Acetogenic Fermentation with Clostridium ljungdahlii
Authors: Infantes Alba , Kugel Michaela , Raffelt Klaus , Neumann Anke
Published: August 2020

Syngas, the product of biomass gasification, can play an important role in moving towards the production of renewable chemical commodities, by using acetogenic bacteria to ferment those gaseous mixtures. Due to the complex and changing nature of biomass, the composition and the impurities present in the final biomass-derived syngas will vary. Because of this, it is important to assess the impact of these factors on the fermentation outcome, in terms of yields, productivity, and product formation and ratio. In this study, Clostridium ljungdahlii was used in a fed-batch fermentation system to analyze the effect of three different biomass-derived syngases, and to compare them to equivalent, clean syngas mixtures. Additionally, four other clean syngas mixtures were used, and the effects on product ratio, productivity, yield, and growth were documented. All biomass-derived syngases were suitable to be used as substrates, without experiencing any complete inhibitory effects. From the obtained results, it is clear that the type of syngas, biomass-derived or clean, had the greatest impact on product formation ratios, with all biomass-derived syngases producing more ethanol, albeit with lesser total productivity.
Botrytis cinerea and Table Grapes: A Review of the Main Physical, Chemical, and Bio-Based Control Treatments in Post-Harvest
Authors: De Simone Nicola , Pace Bernardo , Grieco Francesco , Chimienti Michela , Tyibilika Viwe , Santoro Vincenzo , Capozzi Vittorio , Colelli Giancarlo , Spano Giuseppe , Russo Pasquale
Published: August 2020

Consumers highly appreciate table grapes for their pleasant sensory attributes and as good sources of nutritional and functional compounds. This explains the rising market and global interest in this product. Along with other fruits and vegetables, table grapes are considerably perishable post-harvest due to the growth of undesired microorganisms. Among the microbial spoilers, Botrytis cinerea represents a model organism because of its degrading potential and the huge economic losses caused by its infection. The present review provides an overview of the recent primary physical, chemical, and biological control treatments adopted against the development of B. cinerea in table grapes to extend shelf life. These treatments preserve product quality and safety. This article also focuses on the compliance of different approaches with organic and sustainable production processes. Tailored approaches include those that rely on controlled atmosphere and the application of edible coating and packaging, as well as microbial-based activities. These strategies, applied alone or in combination, are among the most promising solutions in order to prolong table grape quality during cold storage. In general, the innovative design of applications dealing with hurdle technologies holds great promise for future improvements.
Evolution during Three Ripening Stages of Évora Cheese
Authors: P. Carvalho Graça , Santos Rute , Fino Anabela , Ferreira Paulo , M. Rodrigues Francisco , Dias João
Published: August 2020

The variability and heterogeneity found in Évora cheeses, Protected Designation of Origin (PDO), can affect consumers’ choices. Assessing the ripening conditions and their effect can be helpful. To study the effect of ripening duration in Évora cheese PDO, sensory and chemical analyses were performed in cheese samples subjected to 30, 60, and 120 days of ripening under controlled conditions (temperature 14 to 15 °C and humidity 65 to 70%). Sensory analysis was conducted with a homogenous panel previously familiarized with the product after a short training period, and chemical analyses including pH, moisture, NaCl content, aw, and salt-in-moisture were determined. Panelists were able to distinguish the differences in the organoleptic characteristics of the three cheese stages, and chemical determinations showed significant differences between stages. Interrater agreement was higher in the sensory evaluation of cheeses with a longer maturation period. As expected, cheeses in the 120 days ripening period presented lower pH, moisture, and water activity and had higher salt-in-moisture content. This stage received the highest scores in hardness and color of the crust, intensity, pungency of the aroma, intensity of taste and piquancy, and firmness and granular characteristics of texture. Overall acceptance of cheese samples was positive, regardless of the ripening stage, which probably reflects both the homogeneity of taster profiles and the previous knowledge of this particular product. The degree of ripeness influences the physical, chemical, and sensory characteristics but does not affect the acceptance of this product by the consumer.
Biodegradable Hybrid Nanocomposite of Chitosan/Gelatin and Green Synthesized Zinc Oxide Nanoparticles for Food Packaging
Authors: Kumar Santosh , Mudai Abhinab , Roy Barnali , Basumatary Indra Bhusan , Mukherjee Avik , Dutta Joydeep
Published: August 2020

In the context of emerging global concerns with synthetic plastic packaging, alternative natural biodegradable packaging materials are gaining increasing attention for food packaging applications. In this study, chitosan and gelatin nanocomposite hybrid films containing green synthesized zinc oxide (ZnO) nanoparticles (NPs) were developed and microstructural properties were studied. Antimicrobial activity of the developed films was evaluated using both Gram negative (Escherichia coli) and Gram positive bacteria (Staphylococcus aureus). Green synthesis protocol was used for the precipitation of ZnO NPs using fruit extract of Cassia fistula. The as-synthesized polyhedral ZnO NPs were in the range of 20–40 nm (average size ≈29 nm). Reinforcement with ZnO NPs in the hybrid films lead to improved thermal stability, elongation-at-break (EAB), and compactness properties. The developed films with 2% and 4% ZnO NPs showed a smooth, compact, and heterogeneous surface morphology compared to the control (chitosan-gelatin hybrid) films. Disc diffusion assays showed that the nanocomposite film had significant antimicrobial activity against E. coli. The developed hybrid nanocomposite films have potential to be developed as biodegradable alternative for postharvest packaging of fresh fruits and vegetables.
Probiotic Yogurt Fortified with Chickpea Flour: Physico-Chemical Properties and Probiotic Survival during Storage and Simulated Gastrointestinal Transit
Authors: Kaur Sidhu Manwinder , Lyu Fengzhi , Sharkie Thomas Patrick , Ajlouni Said , Ranadheera Chaminda Senaka
Published: August 2020

In the present study, probiotic yogurt with Lactobacillus acidophilus LA5 and Bifidobacterium BB12 was produced via fortification with chickpea flour (0, 1, 2.5, 5% w/v). During refrigerated storage for five weeks, probiotics maintained a viable count above the minimum therapeutic level (106 CFU/g) in all yogurt types. Although there was no significant (p > 0.05) positive effect of chickpea flour on probiotic viability during storage, the addition of chickpea flour has beneficial effects on the viability of both probiotic species in the presence of gastric and intestinal juices, with 0.3% bile. This study also evaluated the physio-chemical properties of probiotic yogurt during storage. Some physicochemical properties of yogurt, such as water holding capacity and susceptibility to syneresis, were enhanced by the addition of chickpea flour. Hence, chickpea flour could be an attractive pulse ingredient in the production of probiotic yogurts for health-conscious consumers.
Basal Levels of CD18 Antigen Presenting Cells in Cow Milk Associate with Copy Number Variation of Fc Gamma Receptors
Authors: Seroussi Eyal , Blum Shlomo E. , Krifucks Oleg , Shirak Andrey , Jacoby Shamay , Leitner Gabriel
Published: August 2020

Differentiation of cells by flow cytometry provides informative somatic cell counts (SCCs) that allow analyzing leukocyte population patterns in udder infections of different etiologies. Postulating that this approach also enhances the statistical power to detect genetic variants linked to cell levels in milk of healthy mammary glands, we used monoclonal antibodies anti-CD18, anti-CD4, anti--CD14, and anti-PMN to count cells presenting these surface antigens, and performed a genome-wide association study of these counts in 125 Israeli Holsteins genotyped using SNP BeadChips. We identified an informative haplotype of 15 SNPs in the centromeric end of BTA3 that was strongly associated with CD18 cells (p < 2.3 × 10−9). Within this region, examination of the network of genes interacting with ITGB2 (CD18) indicated an Fc-γ-receptor gene cluster, including FCGR2A (CD32). Sanger-sequence analysis of FCGR2s-linked exon 3 variation to CD18 counts. Meta-analysis of RNA-Seq data revealed a significant negative correlation (R = −0.51) between expression of CD32 and CD18 in milk. Assembly of DNA-Seq reads uncovered FCGR copy-number variation and a variant, designated V7, was abundant in dairy cattle, probably reflecting adaptation to selection pressure for low SCC in Holstein milk.
Identification of MicroRNA-Related Tumorigenesis Variants and Genes in the Cancer Genome Atlas (TCGA) Data
Authors: Li Chang , Wu Brian , Han Han , Zhao Jeff , Bai Yongsheng , Liu Xiaoming
Published: August 2020

MicroRNAs (miRNAs) are a class of small non-coding RNA that can down-regulate their targets by selectively binding to the 3′ untranslated region (3′UTR) of most messenger RNAs (mRNAs) in the human genome. Single nucleotide variants (SNVs) located in miRNA target sites (MTS) can disrupt the binding of targeting miRNAs. Anti-correlated miRNA–mRNA pairs between normal and tumor tissues obtained from The Cancer Genome Atlas (TCGA) can reveal important information behind these SNVs on MTS and their associated oncogenesis. In this study, using previously identified anti-correlated miRNA–mRNA pairs in 15 TCGA cancer types and publicly available variant annotation databases, namely dbNSFP (database for nonsynonymous SNPs’ functional predictions) and dbMTS (database of miRNA target site SNVs), we identified multiple functional variants and their gene products that could be associated with various types of cancers. We found two genes from dbMTS and 33 from dbNSFP that passed our stringent filtering criteria (e.g., pathogenicity). Specifically, from dbMTS, we identified 23 candidate genes, two of which (BMPR1A and XIAP) were associated with diseases that increased the risk of cancer in patients. From dbNSFP, we identified 65 variants located in 33 genes that were likely pathogenic and had a potential causative relationship with cancer. This study provides a novel way of utilizing TCGA data and integrating multiple publicly available databases to explore cancer genomics.
VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter
Authors: Stuchi Leonardo P. , Castanhole-Nunes Márcia Maria U. , Maniezzo-Stuchi Nathália , Biselli-Chicote Patrícia M. , Henrique Tiago , Padovani Neto João Armando , de-Santi Neto Dalisio , Girol Ana Paula , Pavarino Erika C. , Goloni-Bertollo Eny Maria
Published: August 2020

Deregulation of VEGFA (Vascular Endothelial Growth Factor A) and NFE2L2 (Nuclear Factor (Erythroid-derived 2)-Like 2), involved in angiogenesis and oxidative stress, can lead to thyroid cancer progression. MiR-17-5p and miR-612 are possible regulators of these genes and may promote thyroid disorders. In order to evaluate the involvement of VEGFA, NFE2L2, hsa-miR-17-5p, and hsa-miR-612 in thyroid pathology, we examined tissue samples from colloid goiter, papillary thyroid cancer (PTC), and a normal thyroid. We found higher levels of VEGFA and NFE2L2 transcripts and the VEGFA protein in goiter and PTC samples than in normal tissue. In the goiter, miR-612 and miR-17-5p levels were lower than those in PTC. Tumors, despite showing lower VEGFA mRNA expression, presented higher VEGFA protein levels compared to goiter tissue. In addition, NRF2 (Nuclear Related Transcription Factor 2) protein levels in tumors were higher than those in goiter and normal tissues. Inhibition of miR-17-5p resulted in reduced NFE2L2 expression. Overall, both transcript and protein levels of NFE2L2 and VEGFA were elevated in PTC and colloid goiter. Hsa-miR-612 showed differential expression in PTC and colloid goiter, while hsa-miR-17-5p showed differential expression only in colloid goiter, suggesting that hsa-miR-17-5p may be a positive regulator of NFE2L2 expression in PTC.
Genome-Wide Identification of YABBY Genes in Orchidaceae and Their Expression Patterns in Phalaenopsis Orchid
Authors: Chen You-Yi , Hsiao Yu-Yun , Chang Song-Bin , Zhang Diyang , Lan Si-Ren , Liu Zhong-Jian , Tsai Wen-Chieh
Published: August 2020

The plant YABBY transcription factors are key regulators in the lamina development of lateral organs. Orchid is one of the largest families in angiosperm and known for their unique floral morphology, reproductive biology, and diversified lifestyles. However, nothing is known about the role of YABBY genes in orchids, although biologists have never lost their fascination with orchids. In this study, a total of 54 YABBY genes, including 15 genes in CRC/DL, eight in INO, 17 in YAB2, and 14 in FIL clade, were identified from the eight orchid species. A sequence analysis showed that all protein sequences encoded by these YABBY genes share the highly conserved C2C2 zinc-finger domain and YABBY domain (a helix-loop-helix motif). A gene structure analysis showed that the number of exons is highly conserved in the same clades. The genes in YAB2 clade have six exons, and genes in CRC/DL, INO, and FIL have six or seven exons. A phylogenetic analysis showed all 54 orchid YABBY genes could be classified into four major clades, including CRC/DL, INO, FIL, and YAB2. Many of orchid species maintain more than one member in CRC/DL, FIL, and YAB2 clades, implying functional differentiation among these genes, which is supported by sequence diversification and differential expression. An expression analysis of PhalaenopsisYABBY genes revealed that members in the CRC/DL clade have concentrated expressions in the early floral development stage and gynostemium, the fused male and female reproductive organs. The expression of PeINO is consistent with the biological role it played in ovule integument morphogenesis. Transcripts of members in the FIL clade could be obviously detected at the early developmental stage of the flowers. The expression of three genes, PeYAB2,PeYAB3, and PeYAB4, in the YAB2 clade could be revealed both in vegetative and reproductive tissues, and PeYAB4 was transcribed at a relatively higher level than that of PeYAB2 and PeYAB3. Together, this comprehensive analysis provides the basic information for understanding the function of the YABBY gene in Orchidaceae.
Novel Links between TORC1 and Traditional Non-Coding RNA, tRNA
Authors: Otsubo Yoko , Kamada Yoshiaki , Yamashita Akira
Published: August 2020

Target of rapamycin (TOR) is a serine/threonine kinase that modulates cell growth and metabolism in response to environmental changes. Transfer RNA (tRNA) is an abundant and ubiquitous small non-coding RNA that is essential in the translation of mRNAs. Beyond its canonical role, it has been revealed that tRNAs have more diverse functions. TOR complex 1 (TORC1), which is one of the two TOR complexes, regulates tRNA synthesis by controlling RNA polymerase III. In addition to tRNA synthesis regulation, recent studies have revealed hidden connections between TORC1 and tRNA, which are both essential players in eukaryotic cellular activities. Here, we review the accumulating findings on the regulatory links between TORC1 and tRNA—particularly those links in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe.
Characterization of QTLs and Candidate Genes for Days to Heading in Rice Recombinant Inbred Lines
Authors: Mo Youngjun , Jeong Jong-Min , Ha Su-Kyung , Kim Jinhee , Lee Changmin , Lee Gung Pyo , Jeung Ji-Ung
Published: August 2020

Understanding the gene mechanisms controlling days to heading (DH) is important in rice breeding for adaption in the target environment. Using a recombinant inbred line population derived from the cross between two japonica rice cultivars, Koshihikari and Baegilmi, we identified three consistent quantitative trait loci (QTLs) for DH for two years, qDH3, qDH6, and qDH7 on chromosomes 3, 6, and 7, respectively. While Baegilmi contributed the allele for early heading at qDH6 and qDH7 with the additive effect of five days each, Koshihikari contributed the allele for early heading at qDH3 with the additive effect of three days. Notably, pyramiding two or more alleles for early heading at these QTLs accelerated heading effectively. Sequencing of Hd16, Hd1, and Ghd7, the previously known heading date genes underlying qDH3, qDH6, and qDH7, respectively, revealed that Baegilmi and Koshihikari carry different alleles at the three genes. Molecular markers were developed to screen the allelic compositions of the three genes among 295 Korean commercial rice cultivars. The results showed that few cultivars carry alleles for early heading at the three genes, highlighting that DH can be further accelerated and fine-tuned in breeding programs by combining the desirable alleles of Hd16, Hd1, and Ghd7.
Molecular Pharming of the Recombinant Protein hEGF-hEGF Concatenated with Oleosin Using Transgenic Arabidopsis
Authors: Qiang Weidong , Gao Tingting , Lan Xinxin , Guo Jinnan , Noman Muhammad , Li Yaying , Guo Yongxin , Kong Jie , Li Haiyan , Du Linna , Yang Jing
Published: August 2020

We set out to assess the NIH/3T3 cell proliferation activity of Arabidopsis oil body-expressed recombinant oleosin–hEGF–hEGF protein. Normally, human epidermal growth factor (hEGF) is purified through complex process, however, oleosin fusion technology provides an inexpensive and scalable platform for its purification. Under a phaseolin promoter, we concatenated oleosin gene to double hEGF (hEGF–hEGF) with plant-preferred codons in the expression vectors and the construct was transformed into Arabidopsis thaliana (Arabidopsis). The transgenic Arabidopsis was validated by RT–PCR and the content of recombinant protein oleosin–hEGF–hEGF was quantified by western blot. Subsequently, the proliferation assay and transdermal absorption were determined by MTT method and immunohistochemical staining, respectively. First, the expression level of hEGF was recorded to be 14.83-ng/μL oil body and due to smaller size transgenic oil bodies expressing the recombinant oleosin–hEGF–hEGF, they were more skin permeable than those of control. Second, via the staining intensity of transgenic oil bodies was greater than EGF at all time points via immunohistochemical staining in transdermal absorption process. Lastly, activity assays of oil bodies expressed oleosin–hEGF–hEGF indicated that they stimulated the NIH/3T3 cell proliferation activity. Our results revealed oil-body-expressed oleosin–hEGF–hEGF was potential new material having implications in the field of medicine.
Simulated Video Consultations as a Learning Tool in Undergraduate Nursing: Students’ Perceptions
Authors: Jiménez-Rodríguez Diana , Arrogante Oscar
Published: August 2020

Simulated video consultations, a teaching tool based on high-fidelity simulations, were implemented in response to the necessary adaptation of high-fidelity clinical simulation sessions to the online or virtual modality during the university closure due to the COVID-19 confinement. The purpose of our study was to explore the undergraduate nursing students’ satisfaction and perceptions about simulated video consultations using the high-fidelity simulation methodology. A mixed-method was utilized with 93 undergraduate nursing students using a validated satisfaction questionnaire (quantitative data), which included an observations section (qualitative data). Of the total sample, 97.8% of the students expressed a high overall satisfaction with simulated video consultations, highlighting their practical utility and positive learning outcomes. From the students’ comments, two main themes and their related categories emerged: advantages (satisfaction and enjoyment, learning, and calmness during simulated scenarios), and disadvantages (technical issues and technical skills development). Simulated video consultations may be considered as one more high-fidelity simulation teaching option. Nursing students should be trained in this modality of healthcare to face the challenge brought on by its increased use in healthcare services, beyond the specific adaptation of clinical simulation sessions due to the closure of universities during this pandemic.
Palliative and End-of-Life Care Conversations with Older People with Chronic Obstructive Pulmonary Disease in Croatia—A Pilot Study
Authors: Čičak Petra , Thompson Sanja , Popović-Grle Sanja , Fijačko Vladimir , Lukinac Jasmina , Lukinac Ana Marija
Published: August 2020

Despite the progressive nature of chronic obstructive pulmonary disease (COPD), its association of high morbidity and mortality with severe COPD, and the view that discussions between patients and clinicians about palliative care plans should be grounded in patients’ preferences, many older patients do not receive timely end-of-life care (EOLC) discussions with healthcare professionals (HPs), potentially risking inadequate care at the advanced stages of the disease. The aim of this pilot study was to evaluate EOLC discussions and resuscitation issues as a representative and illustrative part within EOLC in older patients with COPD in the University Hospital Center Osijek, Slavonia (Eastern Region), Croatia, as such data have not yet been explored. The study was designed as cross-sectional research. Two groups of participants, namely, patients at least 65 years old with COPD and healthcare professionals, were interviewed anonymously. In total, 83 participants (22 HPs and 61 patients with COPD) were included in the study. According to the results, 77% of patients reported that they had not had EOLC discussions with HPs, 64% expressed the opinion that they would like such conversations, and the best timing for such discussion would be during frequent hospital admissions. Furthermore, 77% of HPs thought that EOLC communication is important, but only 14% actually discussed such issues with their patients because most of them felt uncomfortable starting such a topic. The majority of older patients with COPD did not discuss advanced care planning with their HPs, even though the majority of them would like to have such a discussion. EOLC between HPs and older patients with COPD should be encouraged in line with patients’ wishes, with the aim to improve their quality of care by anticipating patients’ likely future needs in a timely manner and thereby providing proactive support in accordance with patients’ preferences.
Family and School Context: Effects on the Mental Health of Brazilian Students
Authors: Escobar Daise Fernanda Santos Souza , Jesus Thais Ferreira de , Noll Priscilla Rayanne e Silva , Noll Matias
Published: August 2020

Mental health during adolescence can affect an individual’s long-term quality of life. However, the effects of family and school contexts on students’ mental health have been little explored. This study aims to analyze the relationships between family and school life and feelings of loneliness and trouble sleeping owing to worries in adolescents. The data from this cross-sectional study were obtained from Brazil’s National School Health Survey (PeNSE), which obtained its data through questionnaires. This study’s sample consisted of 102,072 ninth-grade students aged between 11 and 19 years, 52,782 (51.7%) of whom were female, enrolled in public and private schools throughout Brazil. The methodology consisted of an analysis using the Poisson regression model. Regarding the family context, mental health issues were associated with hunger, distant relationships with parents, and family violence. Regarding the school context, feelings of loneliness and trouble sleeping were associated with poor peer relationships, insecurity at school, and schools in more violent areas. This study contributes to the elaboration of public policies aimed at bringing awareness to family members and school bodies that indicators of mental health in adolescents are influenced by the quality of bonds established within these environments.
Perceived Social Discrimination, Socioeconomic Status, and Alcohol Consumption among Chinese Adults: A Nationally Representative Study
Authors: Gu Jiafeng , Ming Xing
Published: August 2020

Perceived social discrimination in China has significant effects on drinking behavior. This finding was reached through multivariate logistic regression analysis of a sample of 22,566 adults in the 2016 China Family Panel Studies (CFPS). This was a cross-sectional study conducted with computer-assisted face-to-face interviews to assess alcohol drinking problems and associated factors among Chinese adults. The proportion of adults prone to alcoholism tends to be higher in eastern than central China, and higher in central than western China. Furthermore, gender discrimination and delays in government interactions as a result of unfair treatment have a positive and significant effect on individuals’ drinking. The alcohol consumption rate among Chinese men is about 13 times that of Chinese women. Additionally, older people have a stronger tendency to drink alcohol. In terms of education, those with lower education levels are more prone to alcoholism than those with higher education levels. Regarding marital status, those who are married are more prone to alcoholism than those who are not. Further, those who have been diagnosed with a chronic disease within the past six months are less prone to alcoholism than those without such diagnosis. People with an annual income between 50,000 and 150,000 yuan are more prone to alcoholism than those with an income under 50,000 yuan. Groups that have experienced unequal treatment in public services are also more prone to alcoholism than those who do not suffer such unequal treatment.
A Multi-Site Study of Traumatic Brain Injury in Mexico and Colombia: Longitudinal Mediational and Cross-Lagged Models of Family Dynamics, Coping, and Health-Related Quality of Life
Authors: Cariello Annahir N. , Perrin Paul B. , Rodríguez-Agudelo Yaneth , Olivera Plaza Silvia Leonor , Quijano-Martinez Maria Cristina , Arango-Lasprilla Juan Carlos
Published: August 2020

Latin America has high rates of traumatic brain injury (TBI), yet reduced mental and physical health outcomes due to limited rehabilitation services. To understand the psychosocial adjustment process in TBI patients in Latin America, the incorporation of cultural values including family-based variables is imperative. The current study examined relations among healthy family dynamics, coping, and mental and physical health related quality of life (HRQL) among a sample of TBI patients across three sites and two countries over the first 4 months post-injury. A sample of 109 acute TBI patients from Mexico and Colombia were recruited, and a series of longitudinal mediation and cross-lag panel analyses were run. Mental and physical HRQL were positively associated with each other, as well as healthy family dynamics and coping. Coping partially mediated the effects of healthy family dynamics on both mental and physical HRQL. Family dynamics showed the strongest cross-lag relationships with coping going from 2 months to 4 months. Similarly, coping showed the strongest cross-lag relationships with both mental and physical HRQL going from baseline to 2 months. Results provide empirical support for the importance of a rehabilitation workforce that has been trained in and can effectively implement family-based interventions for individuals with TBI in Latin America.
Runner’s Perceptions of Reasons to Quit Running: Influence of Gender, Age and Running-Related Characteristics
Authors: Menheere Daphne , Janssen Mark , Funk Mathias , van der Spek Erik , Lallemand Carine , Vos Steven
Published: August 2020

Physical inactivity has become a major public health concern and, consequently, the awareness of striving for a healthy lifestyle has increased. As a result, the popularity of recreational sports, such as running, has increased. Running is known for its low threshold to start and its attractiveness for a heterogeneous group of people. Yet, one can still observe high drop-out rates among (novice) runners. To understand the reasons for drop-out as perceived by runners, we investigate potential reasons to quit running among short distance runners (5 km and 10 km) (n = 898). Data used in this study were drawn from the standardized online Eindhoven Running Survey 2016 (ERS16). Binary logistic regressions were used to investigate the relation between reasons to quit running and different variables like socio-demographic variables, running habits and attitudes, interests, and opinions (AIOs) on running. Our results indicate that, not only people of different gender and age show significant differences in perceived reasons to quit running, also running habits, (e.g., running context and frequency) and AIOs are related to perceived reasons to quit running too. With insights into these related variables, potential drop-out reasons could help health professionals in understanding and lowering drop-out rates among recreational runners.
Risk of Mitral Valve Prolapse in Patients with Keratoconus in Taiwan: A Population-Based Cohort Study
Authors: Chang Yuh-Shin , Tai Ming-Cheng , Weng Shih-Feng , Wang Jhi-Joung , Tseng Sung-Huei , Jan Ren-Long
Published: August 2020

This retrospective, nationwide, matched-cohort study included 4488 new-onset keratoconus (KCN) patients, ≥12 years old, recruited between 2004 and 2011 from the Taiwan National Health Insurance Research Database. The control group included 26,928 non-KCN patients selected from the Taiwan Longitudinal Health Insurance Database 2000. Information for each patient was collected and tracked from the index date until December 2013. The incidence rate of mitral valve prolapse (MVP) was 1.77 times (95% confidence interval (CI) = 1.09–2.88; p = 0.0206) higher in KCN patients ≥40 years old and 1.49 times (95% CI = 1.12–1.98; p = 0.0060) higher in female KCN patients than in controls. After using the Cox proportional hazard regression analysis to adjust for potential confounders, including hypertension, hyperlipidemia, and congestive heart failure, KCN maintained an independent risk factor, MVP being 1.77 times (adjusted hazard ratio (HR) = 1.77, 95% CI = 1.09–2.88) and 1.48 times (adjusted HR = 1.48, 95% CI = 1.11–1.97) more likely to develop in patients ≥40 years old and female patients in the study cohort, respectively. We found that KCN patients ≥40 years of age and female KCN patients have increased risks of MVP. Therefore, it is recommended that KCN patients should be alerted to MVP.