bluuebunny/crossref_metadata_2025 huggingface dataset:
Spotted Hyena Optimization Method for Harvesting Maximum PV Power under Uniform and Partial-Shade Conditions
Authors: Ranganathan Ezhilmaran , Natarajan Rajasekar
Published: April 2022

Maximum power-point-tracking techniques applied for partially shaded photovoltaic array yield maximum power output via operating the panel at its most efficient voltage. Considering the noticeable issues existing with the available methods, including steady-state oscillations, poor tracking capability and complex procedures, a new bioinspired Spotted-Hyena Optimizer (SHO) is proposed. It follows simple implementation steps, and does not require additional controller-parameter tuning to track the optimal power point. To validate the versatility of the proposed method, the SHO algorithm is applied to track the maximum power of different string arrangements under six partial-shade conditions. Further, to authenticate SHO’s methods, its results are compared with perturb-and-observe (P&O), and particle-swarm-optimization (PSO) methods. As a result of its implementation, it is observed that the tracking speed of SHO towards the global convergence for four patterns under 4S2P are 0.34 s, 0.24 s, 0.2 s, and 0.3 s, which is far less than the PSO and P&O methods. Further, to demonstrate its suitability, a hardware prototype is built and tested for various operating conditions. The experimental results are in good agreement with the simulated values.
Temporal Understanding of the Water–Energy Nexus: A Literature Review
Authors: Fontenelle Ana Luiza , Nilsson Erik , Hidalgo Ieda Geriberto , Uvo Cintia B. , Peyerl Drielli
Published: April 2022

Guaranteeing reliable access to water and clean energy has been one of the most debated topics to promote sustainable development, which has made the Water–Energy Nexus (WEN) a relevant field of study. However, despite much development of the WEN, there are still many gaps to be addressed. One of these gaps is the understanding of temporal features. To address this, this study aimed to identify, categorize, and analyze the main temporal features applied in WEN studies based on a review of academic publications from 2010 to 2021. The results showed that most of the recent literature has focused on understanding the WEN from a quantitative perspective, often does not provide clear motivations for their choice of time, and lacks understanding of the role of historical processes. To improve the temporal understanding in WEN research, there is a need to include more methodological diversity, enhance the understanding of historical developments, and diversify the data use. The presented measures provide a chance to improve the evaluation of key issues, enhance the understanding of drivers of trade-offs between the water and energy sectors, and ground the discussion besides quantification. Moreover, these measures help the scientific community better communicate results to a broader audience.
Concept for the Use of Cotton Waste Hydrolysates in Fermentation Media for Biofuel Production
Authors: Binczarski Michal J. , Malinowska Justyna Z. , Berlowska Joanna , Cieciura-Wloch Weronika , Borowski Sebastian , Cieslak Malgorzata , Puchowicz Dorota , Witonska Izabela A.
Published: April 2022

Currently, most cotton textile waste is sent to landfill. However, due to the use of synthetic additives and the chemical treatment of cotton fibers, cotton textile waste is difficult to biodegrade. Cotton textile waste can also be subjected to material recycling, or to incineration/gasification to produce energy. Here, we present the optimization of acid hydrolysis of cotton yarn fibers for glucose efficiency. The cotton yarn hydrolysates showed great potential for replacing simple sugar solutions in fermentation media. The highest glucose concentration was obtained in the hydrolysates of cotton yarn hydrolyzed in a 2% solution of sulfuric acid or phosphoric acid at 140–160 °C for 2 h. After 2 h of hydrolysis at 140 °C with 2% H3PO4, the concentration of glucose in the cotton yarn hydrolysate (13.19 g/L) increased fivefold compared with cotton yarn treated under the same conditions with H2SO4 (2.65 g/L). The structural modifications in the solid residues after acid hydrolysis were analyzed using a scanning electron microscope with energy dispersive spectroscopy (SEM-EDS), attenuated total reflectance Fourier-transform infrared spectroscopy (FTIR-ATR), and Raman spectroscopy. The SEM images, IR spectra, and Raman spectra revealed that the most significant changes in the morphology of the fibers occurred when the process was carried out at high temperatures (≥140 °C). Better growth of the yeast strains Saccharomyces cerevisiae Ethanol Red and Saccharomyces cerevisiae Tokay ŁOCK0204 was observed in the medium containing phosphoric acid hydrolysate. The maximum methane yield of 278 dm3/kgVS and the maximum hydrogen yield of 42 dm/kgVS were reported for cotton yarn waste after pretreatment with H3PO4. This might have been linked to the beneficial effect of phosphorus, which is a key nutrient for anaerobic digestion. The proposed hydrolysis method does not generate fermentation inhibitors.
Recent Advances in Greener and Energy Efficient Alkene Epoxidation Processes
Authors: Mohammed Misbahu Ladan , Saha Basudeb
Published: April 2022

The chemical industry is considered to be one of the largest consumers of energy in the manufacturing sector. As the cost of energy is rising rapidly, coupled with the increasingly stringent standards for the release of harmful chemicals and gases into the environment, more attention is now focused on developing energy efficient chemical processes that could significantly reduce both operational costs and greenhouse gas emissions. Alkene epoxidation is an important chemical process as the resultant epoxides are highly reactive compounds that are used as platform chemicals for the production of commercially important products for flavours, fragrances, paints and pharmaceuticals. A number of epoxidation methods have been developed over the past decade with the ultimate aim of minimising waste generation and energy consumption. In this review paper, some of the recent advances in epoxides synthesis using energy efficient processes are discussed. The epoxidation methods may provide sustainability in terms of environmental impact and energy consumption.
DC and AC Tests of Moisture Electrical Pressboard Impregnated with Mineral Oil or Synthetic Ester—Determination of Water Status in Power Transformer Insulation
Authors: Zukowski Pawel , Rogalski Przemyslaw , Kołtunowicz Tomasz N. , Kierczynski Konrad , Zenker Marek , Pogrebnjak Alexander D. , Kucera Matej
Published: April 2022

In this study, the conductivity and permittivity of electrical pressboard—insulating liquid—water composites were investigated, and the electrical properties of the composites and water were analysed comparatively. Mineral oil and synthetic ester were used as insulating liquids. It was found that the presence of water caused an increase in the permeability of the composite in the frequency range below 100 Hz. The value of static permittivity determined by water in the content of 5 wt. % was approximately 15. To obtain this value caused by liquid water, its volume should be approximately five (oil) and four times (ester) higher than its actual content, respectively. The determined values of the activation energy of the DC conductivity of the composites were several times higher than the values of the activation energy of the conductivity of the liquid water. The experimental values of the dielectric relaxation times were many orders of magnitude higher than the dielectric relaxation times of water. This means that the experimental results obtained for the dielectric permittivity, the activation energy of conductivity and the dielectric relaxation times for moisture electrical pressboard impregnated by mineral oil or synthetic ester exclude the possibility of the presence of liquid water in the composites. It was found that the conductivity of the composites increased exponentially with increasing water content. Such dependencies are characteristic of hopping conductivity, caused by the quantum phenomenon of electron tunnelling between nanometre-sized potential wells. As the increase in conductivity is determined by the presence of water in the composites, therefore, the nanometre potential wells were single-water molecules or nanodrops.
Cost and Cybersecurity Challenges in the Commissioning of Microgrids in Critical Infrastructure: COGE Case Study
Authors: Kraemer Rodrigo Antonio Sbardeloto , Dias Douglas Pereira , da Silva Alisson Carlos , Martins Marcos Aurelio Izumida , Ludwig Mathias Arno
Published: April 2022

The application of microgrids in critical infrastructures has grown considerably due to the power supply reliability and resilience, and the island operation possibility of providing independence from the main grid. However, the necessity of intense information exchange between the devices that compose the microgrid to their proper operation, and the communication infrastructure required to realize that, makes the system vulnerable to cybersecurity threats. In this context, the case study presented in this paper raises two important subjects of discussion in the Brazilian electrical sector context, which are microgrids and cybersecurity in critical infrastructures of the electrical sector. Therefore, this paper presents the practical challenges related to these two subjects by reporting the development, implementation, and commissioning of a new microgrid controller, and the solutions found to accelerate the development by reducing costs, mitigating risks, and optimizing the commissioning time.
Two-Stage Energy Management Strategies of Sustainable Wind-PV-Hydrogen-Storage Microgrid Based on Receding Horizon Optimization
Authors: Wang Jiarui , Li Dexin , Lv Xiangyu , Meng Xiangdong , Zhang Jiajun , Ma Tengfei , Pei Wei , Xiao Hao
Published: April 2022

Hydrogen and renewable electricity-based microgrid is considered to be a promising way to reduce carbon emissions, promote the consumption of renewable energies and improve the sustainability of the energy system. In view of the fact that the existing day-ahead optimal operation model ignores the uncertainties and fluctuations of renewable energies and loads, a two-stage energy management model is proposed for the sustainable wind-PV-hydrogen-storage microgrid based on receding horizon optimization to eliminate the adverse effects of their uncertainties and fluctuations. In the first stage, the day-ahead optimization is performed based on the predicted outpower of WT and PV, the predicted demands of power and hydrogen loads. In the second stage, the intra-day optimization is performed based on the actual data to trace the day-ahead operation schemes. Since the intra-day optimization can update the operation scheme based on the latest data of renewable energies and loads, the proposed two-stage management model is effective in eliminating the uncertain factors and maintaining the stability of the whole system. Simulations show that the proposed two-stage energy management model is robust and effective in coordinating the operation of the wind-PV-hydrogen-storage microgrid and eliminating the uncertainties and fluctuations of WT, PV and loads. In addition, the battery storage can reduce the operation cost, alleviate the fluctuations of the exchanged power with the power grid and improve the performance of the energy management model.
Wood Formation under Changing Environment: Omics Approaches to Elucidate the Mechanisms Driving the Early-to-Latewood Transition in Conifers
Authors: Traversari Silvia , Giovannelli Alessio , Emiliani Giovanni
Published: April 2022

The global change scenarios highlight the urgency of clarifying the mechanisms driving the determination of wood traits in forest trees. Coniferous xylem is characterized by the alternation between earlywood (EW) and latewood (LW), on which proportions the wood density depend, one of the most important mechanical xylem qualities. However, the molecular mechanisms triggering the transition between the production of cells with the typical features of EW to the LW are still far from being completely elucidated. The increasing availability of omics resources for conifers, e.g., genomes and transcriptomes, would lay the basis for the comprehension of wood formation dynamics, boosting both breeding and gene-editing approaches. This review is intended to introduce the importance of wood formation dynamics and xylem traits of conifers in a changing environment. Then, an up-to-date overview of the omics resources available for conifers was reported, focusing on both genomes and transcriptomes. Later, an analysis of wood formation studies using omics approaches was conducted, with the aim of elucidating the main metabolic pathways involved in EW and LW determination. Finally, the future perspectives and the urgent needs on this research topic were highlighted.
Development and Optimization of a Silica Column-Based Extraction Protocol for Ancient DNA
Authors: Dehasque Marianne , Pečnerová Patrícia , Kempe Lagerholm Vendela , Ersmark Erik , Danilov Gleb K. , Mortensen Peter , Vartanyan Sergey , Dalén Love
Published: April 2022

Rapid and cost-effective retrieval of endogenous DNA from ancient specimens remains a limiting factor in palaeogenomic research. Many methods have been developed to increase ancient DNA yield, but modifications to existing protocols are often based on personal experience rather than systematic testing. Here, we present a new silica column-based extraction protocol, where optimizations were tested in controlled experiments. Using relatively well-preserved permafrost samples, we tested the efficiency of pretreatment of bone and tooth powder with a bleach wash and a predigestion step. We also tested the recovery efficiency of MinElute and QIAquick columns, as well as Vivaspin columns with two molecular weight cut-off values. Finally, we tested the effect of uracil-treatment with two different USER enzyme concentrations. We find that neither bleach wash combined with a predigestion step, nor predigestion by itself, significantly increased sequencing efficiency. Initial results, however, suggest that MinElute columns are more efficient for ancient DNA extractions than QIAquick columns, whereas different molecular weight cut-off values in centrifugal concentrator columns did not have an effect. Uracil treatments are effective at removing DNA damage even at concentrations of 0.15 U/µL (as compared to 0.3 U/µL) of ancient DNA extracts.
Depression and Perceived Social Support among Unemployed Youths in China: Investigating the Roles of Emotion-Regulation Difficulties and Self-Efficacy
Authors: Hua Zhiya , Ma Dandan
Published: April 2022

In recent years, the issue of youth unemployment has begun to emerge in China. Unemployed young people are at high risk of depression and other mental health problems. The present study investigates influential factors related to depression and examines the possible mediating effects of difficulties in emotion regulation and self-efficacy between perceived social support and depressive symptoms among unemployed youths in China. Through community recruitment, 511 unemployed young people from Shanghai participated in this cross-sectional survey. The results demonstrate that the prevalence of probable depression in the sample was 49.3% (95% CI: 45.0–53.7%). Moreover, we found that both the perceived social support and self-efficacy were significant negative predictors of depression, whereas difficulties in emotion regulation were positive predictors of depression. In addition, the analysis results indicate that difficulties in emotion regulation and self-efficacy partially mediate the relationship between perceived social support and depression. Overall, this cross-sectional study reveals that depression and mental health problems among China’s unemployed youths are concerning while identifying emotion-regulation difficulties as a risk factor for these and social support and self-efficacy as protective factors, all of which warrant our attention in preventing and intervening with cases of youth depression.
Inverted U-Shaped Relationship between Physical Activity and Academic Achievement among Chinese Adolescents: On the Mediating Role of Physical and Mental Health
Authors: Wang Tianjiao , Guo Congbin
Published: April 2022

We aimed to clarify the relationship between Chinese adolescents’ physical activity and academic achievement, the mediating role of physical and mental health, and provide a scientific reference for the integration of sports and education. Based on baseline data from the China Education Panel Survey, we conducted a nationwide study of 12,960 adolescents. We used nonlinear models, mediation effect analysis, and other measurement methods. Three significant findings emerged: (1) adolescent physical activity duration and academic achievement showed an inverted U-shaped relationship. Moderate physical activity improves academic achievement. The inverted U-shaped inflection point is about 3.549 h/week; (2) physical activity among the male group has a stronger effect on promoting academic achievement, while the positive effect of physical activity among females is not significant. The difference in effectiveness between urban and rural youth groups is relatively small, but the inflection point of rural youth groups is higher, and (3) the level of physical and mental health effectively mediates the inverted U-shaped influence of youth physical activity on academic achievement. We recommend that governments and schools should implement physical activity appropriately and suggest the feasibility of using physical activity to narrow the growth gap between urban and rural youth.
Psychosocial Correlates of Reactive and Proactive Aggression among Protesters during the Social Movement in Hong Kong
Authors: Fung Annis Lai Chu
Published: April 2022

This pioneering study examined how psychosocial factors predicted reactive and proactive aggression among adolescents and young adults in Hong Kong during the Anti-Extradition Bill Movement. A total of 1027 local secondary and tertiary students (578 male, 449 female) aged from 12 to 25 years (M = 16.95, SD = 3.30) completed a questionnaire measuring political participation and attitudes, victimization experiences, aggression, life satisfaction, moral disengagement, and psychopathic traits. ANCOVA and multiple linear regression analyses were performed. The results revealed that compared with non-protesters, protestors had more negative traits and poorer well-being (higher levels of reactive aggression, moral disengagement, narcissism, and impulsivity; lower life satisfaction; more experiences of victimization by strangers related to political disputes). Nonetheless, protesters had similar psychosocial correlates of reactive and proactive aggression when compared to the non-protesters. Among the protesters, reactive aggression was positively predicted by anger towards the government, moral justification, diffusion of responsibility, impulsivity, and narcissism and negatively predicted by satisfaction with the government, advantageous comparison, and dehumanization. Furthermore, proactive aggression was positively predicted by narcissism, euphemistic language, and advantageous comparison and negatively predicted by moral justification. The implications of the findings for psychotherapy, school education, parenting, and social policies are discussed.
Urinary Concentrations of Triclosan, Bisphenol A, and Brominated Flame Retardants and the Association of Triclosan with Demographic Characteristics and Body Fatness among Women with Newly Diagnosed Breast Cancer
Authors: Ilozumba Mmadili N. , Shelver Weilin L. , Hong Chi-Chen , Ambrosone Christine B. , Cheng Ting-Yuan David
Published: April 2022

Background: Triclosan, bisphenol A (BPA), and brominated flame retardants are environmental estrogenic endocrine-disrupting compounds that may influence the prognosis of breast cancer. We examined the urinary concentrations of these compounds and their associations with demographic characteristics and body fatness in a population of women with newly diagnosed breast cancer. Methods: Overnight urine collection and anthropometric measures were obtained from 302 participants. Triclosan, BPA, tetrabromobisphenol A (TBBPA), and tetrabromobenzoic acid (TBBA) concentrations were determined using ultra-performance liquid chromatography–tandem mass spectrometry. Regression analyses were conducted to examine associations of urinary compound concentration with age, menopause, race, ethnicity, educational level, estrogen receptor status, body size, and body composition. Results: Triclosan, BPA, and TBBA were detected in urine samples from 98.3%, 6.0%, and 0.3% of patients, respectively; TBBPA was undetectable. Among patients with quantifiable values, the geometric mean concentrations were 20.74 µg/L (27.04 µg/g creatinine) for triclosan and 0.82 µg/L (1.08 µg/g creatinine) for BPA. Body mass index ≥ 30 vs. <25 kg/m2 was associated with lower creatinine-corrected urinary concentrations of triclosan (−40.00, 95% confidence interval [CI] = −77.19 to −2.81; p = 0.0351). The observed association was predominantly in postmenopausal women (−66.57; 95% CI: −109.18% to −23.96%). Consistent results were found for associations between triclosan levels and fat mass variables. Conclusion: In this study population, women with newly diagnosed breast cancer had triclosan exposure. Assessments of the implications of urinary concentrations of triclosan for women should consider body fatness and menopausal status.
Anemia in Pregnant Women and Children Aged 6 to 59 Months Living in Mozambique and Portugal: An Overview of Systematic Reviews
Authors: Maulide Cane Réka , Chidassicua José Braz , Varandas Luís , Craveiro Isabel
Published: April 2022

Introduction: Globally, anemia is still a public health issue faced by people in low and high-income countries. This study gives an overview of published scientific articles related to the prevalence, nutritional indicators, and social determinants of anemia in pregnant women and children aged 6 to 59 months living in Mozambique and Portugal. Methods: We performed a review of scientific literature in April 2021, searching for published indexed articles in the last 15 years (2003–2018) in electronic databases. Subsequently, quality assessment, data extraction, and content analysis were performed. Results: We have identified 20 relevant publications. Unsurprisingly, anemia plays a relevant role in disability and life imbalances for these subgroups in Mozambique compared with Portugal. For both countries, data on anemia and iron deficiency in pregnant women and children aged 6 to 59 months old are either outdated or remain unclear. Similarly, studies on social determinants and anemia are also still scarce. Conclusions: A gap of information on anemia, other nutritional indicators, and social determinants in pregnant women and children between 6 and 59 months of age living in Mozambique and Portugal is highly observed. More research is crucial to help achieve the goals established by the Sustainable Development Goals.
Reply to Ngoh et al. Comment on “Surendran et al. The Missed Opportunity of Patient-Centered Medical Homes to Thrive in an Asian Context. Int. J. Environ. Res. Public Health 2021, 18, 1817”
Authors: Surendran Shilpa , Foo Chuan De , Tam Chen Hee , Ho Elaine Qiao Ying , Matchar David Bruce , Car Josip , Koh Gerald Choon Huat
Published: April 2022

We carefully read the comment [...]
Sense of Coherence Predicts Physical Activity Maintenance and Health-Related Quality of Life: A 3-Year Longitudinal Study on Cardiovascular Patients
Authors: Adorni Roberta , Greco Andrea , D’Addario Marco , Zanatta Francesco , Fattirolli Francesco , Franzelli Cristina , Maloberti Alessandro , Giannattasio Cristina , Steca Patrizia
Published: April 2022

Cardiovascular disease (CVD) is the leading cause of morbidity and mortality globally. A physically active lifestyle can improve the health-related quality of life (HRQoL) of people with CVD. Nevertheless, adherence to a physically active lifestyle is poor. This study examined the longitudinal (pre-event, 6-, 12-, 24-, and 36-month follow-ups) physical activity profiles in 275 patients (mean age = 57.1 years; SD = 7.87; 84% men) after the first acute coronary event. Moreover, it investigated the associations among physical activity, sense of coherence (SOC), and HRQoL. Physical activity profiles were identified through latent class growth analysis, and linear regressions were then performed to explore the association between physical activity, SOC, and HRQoL. After the cardiovascular event, 62% of patients reached adequate physical activity levels and maintained them over time (virtuous profile). The remaining 38% could not implement (23%) or maintain (15%) a healthy behavior. A strong SOC at baseline (standardized β = 0.19, p = 0.002) predicted the probability of belonging to the virtuous profile. Moreover, a strong SOC at baseline (standardized β = 0.27, p < 0.001), together with the probability of belonging to the virtuous profile (standardized β = 0.16, p = 0.031), predicted a better HRQoL at the final follow-up. Findings showed a strong relationship between SOC, the ability to adopt a physically active lifestyle stably over time, and HRQoL in patients with CVD. They suggest the importance of tailoring physical activity interventions by promoting resilience resources such as SOC to improve patients’ quality of life after an acute coronary event.
Polychlorinated Biphenyls and Pulmonary Hypertension
Authors: Assaggaf Hamza , Yoo Changwon , Lucchini Roberto G. , Black Steven M. , Hamed Munerah , Minshawi Faisal , Felty Quentin
Published: April 2022

Polychlorinated biphenyls (PCBs) are persistent environmental pollutants that were banned because of their potential carcinogenicity. Population studies have shown that PCBs are associated with lung toxicity and hypertension. The objective of this study was to evaluate whether higher exposure to PCB congeners is associated with the risk of pulmonary hypertension. Serum levels of PCBs in 284 subjects with combined risk factors for pulmonary arterial hypertension (PAH) were compared to 4210 subjects with no risk for PAH using the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2004. The major findings from this study include significantly higher PCB levels in PAH subjects compared to non-PAH subjects; for example, the geometric mean (GM) of PCB74 was 15.91 (ng/g) (14.45–17.53) vs. 11.48 (ng/g) (10.84–12.16), respectively. Serum levels of PCB congeners showed an increasing trend in the age group 20–59 years as PCB180 GM was 19.45 (ng/g) in PAH vs. 12.75 (ng/g) in the control. A higher body burden of PCB153 followed by PCB138, PCB180, and PCB118 was observed. Estimated age, race, BMI, and gender-adjusted ORs for PCB congener levels in subjects with the combined risk factors for PAH compared to controls was significant; for example, PCB99 (OR: 1.5 (CI: 1.49–1.50). In summary, these findings indicate that exposure, as well as body burden estimated based on lipid adjustment of PCBs, were higher in people with risk factors for PAH, and PCB congeners accumulated with age. These findings should be interpreted with caution because of the use of cross-sectional self-reported data and a small sample size of subjects with combined risk factors for pulmonary arterial hypertension. Nonetheless, our finding emphasizes a need for a comprehensive environmental molecular epidemiologic study to determine the potential role of environmental exposures to PCBs in the development of pulmonary arterial hypertension.
Reduced Muscle Activity of the Upper Extremity in Individuals with Spinal Cord Injuries
Authors: Lee Kyung-Sun , Zaman Mobasshira , Hwang Jaejin
Published: April 2022

Compromised physical ability due to musculoskeletal impairment among spinal cord injury (SCI) patients is known to negatively affect their quality of life. It is essential to comprehensively understand the muscle strength of the upper extremity among patients with SCI to enhance muscle function and capacity to engage in an active lifestyle. The objective of this study was to evaluate the muscle strength of 15 upper extremity muscles among patients with SCI and compare the relative weakness of individual muscles to the control group. Seven male patients with SCI with ASIA impairment scale D and E and 33 males in the control group participated in this study. Each participant performed maximal voluntary contraction of individual muscles, and the electromyography data were recorded. The results showed that the majority of the upper extremity muscles (12 out of 15) showed considerable weakness (24 to 53%) relative to the control group. Furthermore, the relative strength (ranking) of individual muscles among 15 upper extremity muscles was different between patients with SCI and the control group. This information would be useful to the selective strengthening of specific muscles as an intensive rehabilitation effort and prevent overuse and adverse injuries due to excessive muscle training.
Profile of Whole Body Electromyostimulation Training Users—A Pilot Study
Authors: Rodrigues-Santana Luiz , Louro Hugo , Denche-Zamorano Ángel , Vega-Muñoz Alejandro , Contreras-Barraza Nicolás , Adsuar Jose Carmelo
Published: April 2022

(1) Introduction: Whole Body Electromyostimulation is a technological and time efficient personal training practiced all over the world. With the increase of practitioners in the last 10 years, the need to study more about practitioners has arisen, so this pilot study aims to trace a user profile of this method through the analysis of socio-demographic data for a better understand of the profile of people looking for this type of training to improve the effectiveness of the intervention and develop programs that are in accordance with the motivation of practitioners. (2) Methods: 270 users from 5 countries answered an online questionnaire with socio-demographic questions. Data were treated using descriptive statistics. Possible differences between sexes and between groups were analyzed by means of non-parametric statistical tests: Mann–Whitney U-test (continuous variables); in addition to studying possible dependence relationships and differences between proportions, using the Chi-square statistic with pairwise z-test using the Bonferroni correction (categorical variables). (3) Results: Middle-aged women are the main user of this type of training. The majority of WB-EMS users do another type of physical activity with significant difference between men and woman (p < 0.05) men are more active than women. Weight loss, health and wellness and muscle mass increase are the main goals of the WB-EMS users. There are significant differences in weight loss and rehabilitation between genders (p < 0.05). Women look much more than men to lose weight and men look more than women to rehabilitation. (4) Conclusions: The user profile is a physically active woman, aged 35–49 years, with normal weight and high educational level, who carries out twice weekly full body electrostimulation training with the goals of weight loss, health and/or wellness and muscle mass gain.
Effect of Treatment and Nutrition on Glycemic Variability in Critically Ill Patients
Authors: Kapłan Cezary , Kalemba Alicja , Krok Monika , Krzych Łukasz
Published: April 2022

Nondiabetic hyperglycemia is a dangerous metabolic phenomenon in the intensive care unit. Inattentive treatment of glycemic disorders is a serious health hazard promoting negative outcomes. The aim of our study was to assess glycemic variability and its basic determinants, and to verify its relationship with mortality in patients hospitalized in a mixed ICU (intensive care unit). The medical records of 37 patients hospitalized 13 January–29 February 2020 were analyzed prospectively. The BG (blood glucose) variability during the stay was assessed using two definitions, i.e., the value of standard deviation (SD) from all the measurements performed and the coefficient of variation (CV). A correlation between the BG variability and insulin dose was observed (SD: R = 0.559; p < 0.01; CV: R = 0.621; p < 0.01). There was also a correlation between the BG variability and the total energy daily dose (SD: R = 0.373; p = 0.02; CV: R = 0.364; p = 0.03). Glycemic variability was higher among patients to whom treatment with adrenalin (p = 0.0218) or steroid (p = 0.0292) was applied. The BG variability, expressed using SD, was associated with ICU mortality (ROC = 0.806; 95% CI: 0.643–0.917; p = 0.0014). The BG variability in the ICU setting arises from the loss of balance between the supplied energy and the applied insulin dose and may be associated with a worse prognosis.
Investigating Perceptions of Teachers and School Nurses on Child and Adolescent Oral Health in Los Angeles County
Authors: Maida Carl A. , Marcus Marvin , Xiong Di , Ortega-Verdugo Paula , Agredano Elizabeth , Huang Yilan , Zhou Linyu , Lee Steve Y. , Shen Jie , Hays Ron D. , Crall James J. , Liu Honghu
Published: April 2022

This study reports the results of focus groups with school nurses and teachers from elementary, middle, and high schools to explore their perceptions of child and adolescent oral health. Participants included 14 school nurses and 15 teachers (83% female; 31% Hispanic; 21% White; 21% Asian; 14% African American; and 13% Others). Respondents were recruited from Los Angeles County schools and scheduled by school level for six one-hour focus groups using Zoom. Audio recordings were transcribed, reviewed, and saved with anonymization of speaker identities. NVivo software (QSR International, Melbourne, Australia) was used to facilitate content analysis and identify key themes. The nurses’ rate of “Oral Health Education” comments statistically exceeded that of teachers, while teachers had higher rates for “Parental Involvement” and “Mutual Perception” comments. “Need for Care” was perceived to be more prevalent in immigrants to the United States based on student behaviors and complaints. “Access to Care” was seen as primarily the nurses’ responsibilities. Strong relationships between community clinics and schools were viewed by some as integral to students achieving good oral health. The results suggest dimensions and questions important to item development for oral health surveys of children and parents to address screening, management, program assessment, and policy planning.
Structural Requirements for the Binding of a Peptide to Prohibitins on the Cell Surface of Monocytes/Macrophages
Authors: Zhang Qindong , Olberg Anniken , Sioud Mouldy
Published: April 2022

The screening of phage peptide libraries resulted in the identification of a sequence (named NW peptide, NWYLPWLGTNDW) that specifically binds to human monocytes and macrophages. Although the NW peptide can be used for the targeted delivery of therapeutics without knowledge of its receptor(s), the identification of-its binding partners will support future clinical applications-Here, we used the biotinylated NW peptide for cross-linking cell surface receptor(s) on live cells or as bait in pull-down assays with membrane proteins isolated from monocytes or human THP-1 cells differentiated into macrophages. Proteomic analysis of the captured proteins identified cell surface prohibitins (PHB1 and PHB2) and modified albumin as binding partners. Using flow cytometry and pull-down methods, we demonstrated that PHB1 and PHB2 interact directly with the NW peptide. Confocal imaging showed co-localization of the peptide with PHB1 on the surface of monocytes. Single replacement of either tryptophan or leucine with alanine completely inhibited binding, whereas the replacement of asparagine at position 1 or 10 and aspartic acid at position 11 with alanine did not affect the binding of the peptide variants. Neutral amino acid replacement of tryptophan at positions 2, 6, and 12 with tyrosine or phenylalanine also abolished the binding, implying that the indole ring of tryptophan is indispensable for the NW peptide to bind. Overall, the data suggest that membrane-associated prohibitins might be a useful target for the delivery of therapeutics to monocytes/macrophages and that tryptophan and leucine are key residues for peptide binding.
Editorial of Special Issue “The Role of Vitamin D in Human Health and Diseases”
Authors: Silvagno Francesca , Bergandi Loredana
Published: April 2022

Vitamin D has been described as a differentiative hormone, but this definition is reductive for a molecule targeting every tissue, produced in its active form by many kinds of cells and effective on the whole life of cells by different mechanisms, which lead to nuclear, non-genomic and mitochondrial effects [...]
Liquid Biopsy as a Source of Nucleic Acid Biomarkers in the Diagnosis and Management of Lynch Syndrome
Authors: Buglyó Gergely , Styk Jakub , Pös Ondrej , Csók Ádám , Repiska Vanda , Soltész Beáta , Szemes Tomas , Nagy Bálint
Published: April 2022

Lynch syndrome (LS) is an autosomal dominant inherited cancer predisposition disorder, which may manifest as colorectal cancer (CRC), endometrial cancer (EC) or other malignancies of the gastrointestinal and genitourinary tract as well as the skin and brain. Its genetic cause is a defect in one of the four key DNA mismatch repair (MMR) loci. Testing of patients at risk is currently based on the absence of MMR protein staining and detection of mutations in cancer tissue and the germline, microsatellite instability (MSI) and the hypermethylated state of the MLH1 promoter. If LS is shown to have caused CRC, lifetime follow-up with regular screening (most importantly, colonoscopy) is required. In recent years, DNA and RNA markers extracted from liquid biopsies have found some use in the clinical diagnosis of LS. They have the potential to greatly enhance the efficiency of the follow-up process by making it minimally invasive, reproducible, and time effective. Here, we review markers reported in the literature and their current clinical applications, and we comment on possible future directions.
Titanium Surface Characteristics Induce the Specific Reprogramming of Toll-like Receptor Signaling in Macrophages
Authors: González-Sánchez Zaira , Areal-Quecuty Victoria , Jimenez-Guerra Alvaro , Cabanillas-Balsera Daniel , Gil Francisco Javier , Velasco-Ortega Eugenio , Pozo David
Published: April 2022

Most of the research on titanium-based dental implants (Ti-discs) is focused on how they are able to stimulate the formation of new tissue and/or cytotoxic studies, with very scarce data on their effects on functional responses by immunocompetent cells. In particular, the link between the rewiring of innate immune responses and surface biomaterials properties is poorly understood. To address this, we characterize the functional response of macrophage cultures to four different dental titanium surfaces (MA: mechanical abrasion; SB + AE: sandblasting plus etching; SB: sandblasting; AE: acid etching). We use different Toll-like receptor (TLR) ligands towards cell surface receptors (bacterial lipopolysaccharide LPS for TLR4; imiquimod for TLR7; synthetic bacterial triacylated lipoprotein for TLR2/TLR1) and endosomal membrane receptor (poly I:C for TLR3) to simulate bacterial (cell wall bacterial components) or viral infections (dsRNA and ssRNA). The extracellular and total LDH levels indicate that exposure to the different Ti-surfaces is not cytotoxic for macrophages under resting or TLR-stimulated conditions, although there is a tendency towards an impairment in macrophage proliferation, viability or adhesion under TLR4, TLR3 and TLR2/1 stimulations in SB discs cultures. The secreted IL-6 and IL-10 levels are not modified upon resting macrophage exposure to the Ti-surfaces studied as well as steady state levels of iNos or ArgI mRNA. However, macrophage exposure to MA Ti-surface do display an enhanced immune response to TLR4, TLR7 or TLR2/1 compared to other Ti-surfaces in terms of soluble immune mediators secreted and M1/M2 gene expression profiling. This change of characteristics in cellular phenotype might be related to changes in cellular morphology. Remarkably, the gene expression of Tlr3 is the only TLR that is differentially affected by distinct Ti-surface exposure. These results highlight the relevance of patterned substrates in dental implants to achieve a smart manipulation of the immune responses in the context of personalized medicine, cell-based therapies, preferential lineage commitment of precursor cells or control of tissue architecture in oral biology.
Accelerated Generation of Extra-Islet Insulin-Producing Cells in Diabetic Rats, Treated with Sodium Phthalhydrazide
Authors: Abidov Musa T. , Sokolova Ksenia V. , Gette Irina F. , Danilova Irina G.
Published: April 2022

β-cells dysfunction plays an important role in the pathogenesis of type 2 diabetes (T2D), partially may be compensated by the generation of extra-islet insulin-producing cells (IPCs) in pancreatic acini and ducts. Pdx1 expression and inflammatory level are suggested to be involved in the generation of extra-islet IPCs, but the exact reasons and mechanisms of it are unclear. Macrophages are key inflammatory mediators in T2D. We studied changes in mass and characteristics of extra-islet IPCs in rats with a streptozotocin-nicotinamide model of T2D and after i.m. administration of 20 daily doses of 2 mg/kg b.w. sodium aminophthalhydrazide (APH). Previously, we found that APH modulates macrophage production and increases the proliferative activity of pancreatic β-cells. Expressions of insulin and Pdx1, as well as F4/80 (macrophage marker), were detected at the protein level by immunohistochemistry analysis, the concentration of pro- and anti-inflammatory cytokines in blood and pancreas—by ELISA. Diabetic rats treated with APH showed an increasing mass of extra-islet IPCs and the content of insulin in them. The presence of Pdx1+ cells in the exocrine pancreas also increased. F4/80+ cell reduction was accompanied by increasing TGF-β1 content. Interestingly, during the development of diabetes, the mass of β-cells decreased faster than the mass of extra-islet IPCs, and extra-islet IPCs reacted to experimental T2D differently depending on their acinar or ductal location.
Synergistic Effects of Weighted Genetic Risk Scores and Resistin and sST2 Levels on the Prognostication of Long-Term Outcomes in Patients with Coronary Artery Disease
Authors: Chou Hsin-Hua , Hsu Lung-An , Juang Jyh-Ming Jimmy , Chiang Fu-Tien , Teng Ming-Sheng , Wu Semon , Ko Yu-Lin
Published: April 2022

Resistin and soluble suppression of tumorigenicity 2 (sST2) are useful predictors in patients with coronary artery disease (CAD). Their serum levels are significantly attributed to variations in RETN and IL1RL1 loci. We investigated candidate variants in the RETN locus for resistin levels and those in the IL1RL1 locus for sST2 levels and evaluated the prognostication of these two biomarkers and the corresponding variants for long-term outcomes in the patients with CAD. We included 4652, 557, and 512 Chinese participants from the Taiwan Biobank (TWB), cardiovascular health examination (CH), and CAD cohorts, respectively. Candidate variants in RETN and IL1RL1 were investigated using whole-genome sequence (WGS) and genome-wide association study (GWAS) data in the TWB cohort. The weighted genetic risk scores (WGRS) of RETN and IL1RL1 with resistin and sST2 levels were calculated. Kaplan–Meier curves were used to analyze the prognostication of resistin and sST2 levels, WGRS of RETN and IL1RL1, and their combinations. Three RETN variants (rs3219175, rs370006313, and rs3745368) and two IL1RL1 variants (rs10183388 and rs4142132) were independently associated with resistin and sST2 levels as per the WGS and GWAS data in the TWB cohort and were further validated in the CH and CAD cohorts. In combination, these variants explained 53.7% and 28.0% of the variation in resistin and sST2 levels, respectively. In the CAD cohort, higher resistin and sST2 levels predicted higher rates of all-cause mortality and major adverse cardiac events (MACEs) during long-term follow-up, but WGRS of RETN and IL1RL1 variants had no impact on these outcomes. A synergistic effect of certain combinations of biomarkers with RETN and IL1RL1 variants was found on the prognostication of long-term outcomes: Patients with high resistin levels/low RETN WGRS and those with high sST2 levels/low IL1RL1 WGRS had significantly higher all-cause mortality and MACEs rates, and those with both these combinations had the poorest outcomes. Both higher resistin and sST2 levels, but not RETN and IL1RL1 variants, predict poor long-term outcomes in patients with CAD. Furthermore, combining resistin and sST2 levels with the WGRS of RETN and IL1RL1 genotyping exerts a synergistic effect on the prognostication of CAD outcomes. Future studies including a large sample size of participants with different ethnic populations are needed to verify this finding.
The Inhibitory Response to PI3K/AKT Pathway Inhibitors MK-2206 and Buparlisib Is Related to Genetic Differences in Pancreatic Ductal Adenocarcinoma Cell Lines
Authors: Ma Yixuan , Sender Sina , Sekora Anett , Kong Weibo , Bauer Peter , Ameziane Najim , Al-Ali Ruslan , Krake Susann , Radefeldt Mandy , Weiss Frank Ulrich , Lerch Markus M. , Parveen Alisha , Zechner Dietmar , Junghanss Christian , Murua Escobar Hugo
Published: April 2022

The aberrant activation of the phosphoinositide 3-kinase (PI3K)/ protein kinase B (AKT) pathway is common in pancreatic ductal adenocarcinomas (PDAC). The application of inhibitors against PI3K and AKT has been considered as a therapeutic option. We investigated PDAC cell lines exposed to increasing concentrations of MK-2206 (an AKT1/2/3 inhibitor) and Buparlisib (a pan-PI3K inhibitor). Cell proliferation, metabolic activity, biomass, and apoptosis/necrosis were evaluated. Further, whole-exome sequencing (WES) and RNA sequencing (RNA-seq) were performed to analyze the recurrent aberrations and expression profiles of the inhibitor target genes and the genes frequently mutated in PDAC (Kirsten rat sarcoma virus (KRAS), Tumor protein p53 (TP53)). MK-2206 and Buparlisib demonstrated pronounced cytotoxic effects and limited cell-line-specific effects in cell death induction. WES revealed two sequence variants within the direct target genes (PIK3CA c.1143C > G in Colo357 and PIK3CD c.2480C > G in Capan-1), but a direct link to the Buparlisib response was not observed. RNA-seq demonstrated that the expression level of the inhibitor target genes did not affect the efficacy of the corresponding inhibitors. Moreover, increased resistance to MK-2206 was observed in the analyzed cell lines carrying a KRAS variant. Further, increased resistance to both inhibitors was observed in SU.86.86 carrying two TP53 missense variants. Additionally, the presence of the PIK3CA c.1143C > G in KRAS-variant-carrying cell lines was observed to correlate with increased sensitivity to Buparlisib. In conclusion, the present study reveals the distinct antitumor effects of PI3K/AKT pathway inhibitors against PDAC cell lines. Aberrations in specific target genes, as well as KRAS and TP53, individually or together, affect the efficacy of the two PI3K/AKT pathway inhibitors.