bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Diversity and Connectivity of the Vulnerable Species Phengaris nausithous in Palencia (Northern Spain)
Authors: Sánchez-Sastre Luis Fernando , Ramírez-del-Palacio Óscar , Martín-Ramos Pablo , Hernández-Minguillón María-Ángeles
Published: February 2025

Habitat fragmentation is one of the most critical threats to species conservation, particularly for those with strict habitat preferences and specialized feeding and reproductive behaviors. In such cases, dispersal is essential for gene flow, playing a significant role in determining metapopulation dynamics and stability. This is precisely the case for many Lepidoptera, such as the dusky large blue butterfly (Phengaris nausithous), whose larvae feed on a single host plant (Sanguisorba officinalis) before completing their life cycle as social parasites of Myrmica ants. In Spain, P. nausithous is a vulnerable species found only in certain areas along the mountainous fringe of the northern plateau. In one of these regions (northern Palencia province), five populations separated by distances ranging from 2 to 40 km were studied using microsatellite markers, along with a control population located 200 km away (Soria province), to assess their genetic structure and population dynamics. The results revealed a lack of connectivity and pronounced genetic structuring in four of the studied populations. Only two populations within the Montaña Palentina Natural Park exhibited clear connectivity, demonstrating high gene flow between them. This could be explained by the availability and stability of S. officinalis patches, which may function as stepping stones. This study confirms the challenges posed by habitat fragmentation but also demonstrates that Phengaris nausithous can maintain gene flow between relatively distant areas. At a local level, the results provide the first evidence that the populations studied within the western part of the Montaña Palentina Natural Park belong to a single metapopulation, which may encompass the entire natural park.
Hermetic Bags Effectively Manage Emerging and Common Pests of Stored Cowpeas in Niger
Authors: Bawa Habibou Yahaya Dan , Baoua Ibrahim Boukary , Rabé Mahamane Moctar , Baributsa Dieudonne
Published: February 2025

The cowpea is a vital crop for low-resource farmers in the Sahel, but post-harvest losses due to insect pests remain a major challenge. Callosobruchus maculatus (Fabricius, 1775), is the primary pest responsible for most of the damage to stored cowpeas. Recently, Trogoderma granarium (Everts, 1898) was found infesting cowpeas in large warehouses in Niger. This study evaluated hermetic storage bags to manage both common and emerging insect pests. Treatments included (i) the Purdue Improved Crop Storage (PICS) hermetic bag; (ii) a woven polypropylene (PP) bag with a polyethylene (PE) liner and Phostoxin; and (iii) a woven PP bag without Phostoxin (control). Naturally infested cowpea grains were obtained from the Office des Produits Vivriers du Niger (OPVN) warehouse in Maradi, Niger. Infestation levels were assessed using 12 samples of 500 g each, randomly collected from each treatment at the start and end of the trial. Major pests identified were C. maculatus, T. granarium, and Tribolium sp., with initial populations of 0.83, 0.44, and 0.83 adults per 500 g of cowpea, respectively. After six months of storage, pest densities in the control increased significantly: 232-fold for C. maculatus, 7.4-fold for T. granarium, and 2.7-fold for Tribolium sp.; resulting in a 38.5% weight loss. In contrast, both the Phostoxin and the PICS hermetic bags effectively suppressed pest populations, preventing weight loss. This study confirms the efficacy of hermetic storage, such as the PICS bag, in protecting cowpeas from both common and emerging pests.
Methodology for the Evaluation of Varietal Resistance to Haplaxius crudus, Vector of the Causal Agent of Lethal Wilt in Oil Palm in Colombia
Authors: Beltrán-Aldana Ivette Johana , Romero-Guerrero Gladys Alejandra , Mesa-Fuquen Eloina , Morales-Rodriguez Anuar
Published: February 2025

Lethal Wilt is a limiting disease for oil palm cultivation in the eastern and central zones of Colombia. In the eastern zone, it caused the eradication of approximately 8700 ha of oil palm between 2010 and 2022, with economic losses of more than 185 million dollars. Studies conducted by Cenipalma reported that the pathogen causing this disease is Candidatus Liberibacter, which is possibly transmitted by Haplaxius crudus (Van Duzee). The adults feed on the foliage of the palms and move between them, spreading the pathogen in the plantation. A strategy to contribute to the management of LW is establishing cultivars resistant to the insect vector; however, no resistant cultivars or sources of resistance have been identified in the country’s commercial cultivars or germplasm collections. Therefore, this work aimed to design and validate a methodology to characterize the oil palm genotypes Elaeis guineensis and Elaeis oleifera and interspecific OxG hybrids against adults of H. crudus, evaluating resistance through antixenosis and antibiosis to identify genotypes with possible sources of resistance. An arena with leaflets of the different genotypes in free-choice tests was used to assess antixenosis. For antibiosis, entomological sleeves were installed on the palm leaves, which were infested with adults of H. crudus from a breeding unit. The results of antixenosis and antibiosis in both the first phase (design) and the second phase (validation) indicated greater preference and survival for the genotypes of E. guineensis and lower preference and survival for the interspecific hybrids and E. oleifera. In the genotype E. guineensis, the average mortality was reached after 30 days, while in E. oleifera and the hybrids, it occurred between the third and fourth days. The results of this research provide a reproducible methodology for the evaluation of oil palm germplasms against H. crudus and sucking insects for the selection of sources of resistance for incorporation into breeding programs.
Prevalence of ADHD Among Nonpsychotic Patients of Day-Care Centers and Comparison of Psychiatric Comorbidities Among Persons With and Without ADHD: A Pilot Study
Authors: Szaniawska Monika , Kokoszka Andrzej
Published: February 2025

Background/Objectives: Adults with ADHD are more likely to develop other mental disorders. There are few data on the prevalence of ADHD among nonpsychotic patients treated with psychotherapy in day-care centers. This paper aims to assess persons with ADHD in this specific population and to compare psychiatric comorbidities in groups with and without ADHD. Methods: A total of 152 persons (men and women aged 18–58 years [M = 33; SD = 9.56]) were diagnosed according to the Mini International Neuropsychiatric Interview 5.0. (MINI). Personality disorders were assessed with SCID-II. ADHD was diagnosed according to a structured diagnostic history, DSM-IV-TR criteria, as well as CAARS and ASRS questionnaires. Results: A total of 47 persons (31%) met the criteria for ADHD in adults. Individuals with and those without ADHD did not differ in terms of education, employment, earnings, functioning on the labor market, satisfaction with their material status, being in a relationship, and divorce rate. Patients with ADHD more often had comorbidities such as major depression (34%) and personality disorders (31.9%), while for those without ADHD, anxiety disorders (58.1%) were predominant. The prevalence of personality disorders was higher in the group with ADHD and approached statistical significance (χ2 [1, N = 152] = 18.496; p = 0.06). Borderline and passive–aggressive personality disorders were also more frequent in this group. Conclusions: The prevalence of ADHD among patients treated in the day-care psychiatric center was much higher than in general population. In the group with ADHD, there was a higher prevalence of personality disorders, mainly borderline and passive–aggressive types. As only a small sample was analyzed in this single-center study, the presented findings need replication in similar settings.
Outcome of Facial Nerve Integrity After Parotid Gland Surgery With and Without Intraoperative Monitoring: A Ten-Year Retrospective Study
Authors: Cirignaco Giulio , Monarchi Gabriele , Betti Enrico , Paglianiti Mariagrazia , Catarzi Lisa , Tel Alessandro , Vaira Luigi Angelo , Balercia Paolo , Consorti Giuseppe
Published: February 2025

Background: Facial nerve injury is one of the most concerning complications of parotid gland surgery, with temporary and permanent dysfunction rates varying widely in the literature. This study aimed to identify factors associated with facial nerve injury during surgery for benign parotid tumors and assess the protective efficacy of intraoperative nerve monitoring (NIM) in preventing nerve injury. Methods: This retrospective study analyzed 329 patients who underwent parotid gland surgery between 2010 and 2023. Data collected included patient demographics, tumor characteristics (size, location, histology), surgical parameters (operation time, NIM usage), and postoperative nerve function evaluated using a modified House–Brackmann scale. Descriptive and inferential statistical analyses, including Chi-square tests and logistic regression, were employed to identify predictors of facial nerve outcomes. Results: Facial nerve injury occurred in 5.2% of patients, comprising 4.6% temporary and 0.6% permanent damage. Tumors located in the deep and inferior lobes significantly increased the risk of facial paralysis/palsy (p = 0.035), while tumor size and histology showed no significant associations. Using NIM significantly reduced the risk of facial nerve injury (p < 0.05; OR: 0.35, 95% CI: 0.25–0.50). Age was also identified as a significant predictor of nerve dysfunction (p < 0.05). Conclusions: The findings emphasize the importance of NIM in mitigating facial nerve injury, especially in anatomically complex tumor locations. Tailored surgical approaches based on tumor location and patient-specific factors, combined with the routine use of NIM, are recommended to optimize nerve preservation and improve postoperative outcomes.
Sundowning Syndrome in Dementia: Mechanisms, Diagnosis, and Treatment
Authors: Reimus Michalina , Siemiński Mariusz
Published: February 2025

“Sundowning syndrome” refers to the evening decline in mental state among cognitively impaired patients. This phenomenon is well known, but it is not entirely understood. Its prevalence ranges from 1.6% to 66% of patients with dementia. Development of SS relies on neurodegeneration, the presence of sleep disorders, circadian rhythm of patients’ activities, and mood disorders. Therefore, patients with SS need very precise diagnostic workup aiming at defining the exact cause of the syndrome. Potential therapeutic modalities include behavioral and environmental interventions and pharmacological approaches. Pharmacotherapy with sedatives can by effective but is related to severe side effects. Behavioral interventions are more efficacious but require intense involvement of caregivers. This article discusses the biological processes that may underlie SS and proposes potential diagnostic procedures and therapeutic interventions.
Multidisciplinary Therapeutic Approaches to Pancreatic Cancer According to the Resectability Status
Authors: Mauro Aurelio , Faverio Carlotta , Brizzi Leonardo , Mazza Stefano , Scalvini Davide , Alfieri Daniele , Cappellini Alessandro , Chicco Fabio , Ciccioli Carlo , Delogu Claudia , Bardone Marco , Gallotti Anna , Pagani Anna , Torello Viera Francesca , Anderloni Andrea
Published: February 2025

Pancreatic ductal adenocarcinoma (PDAC) is among the most lethal cancers, characterized by late diagnosis, rapid progression, and limited therapeutic options. Despite advancements, only 20% of patients are eligible for surgical resection at diagnosis, the sole curative treatment. Multidisciplinary evaluation is critical to optimize care, stratifying patients based on resectability into resectable, borderline resectable, locally advanced, and metastatic stages. Preoperative imaging, such as computed tomography (CT) and endoscopic ultrasound (EUS), remains central for staging, for vascular assessment, and tissue acquisition. Endoscopic and systemic approaches are pivotal for addressing complications like biliary obstruction and improving outcomes. Endoscopic retrograde cholangiopancreatography (ERCP) has been considered for years the gold standard for biliary drainage, although EUS-guided drainage is increasingly utilized due to its efficacy in both resectable and unresectable disease. Systemic therapies play a key role in neoadjuvant, adjuvant, and palliative settings, with ongoing trials exploring their impact on survival and resectability chance. This review highlights the evolving multidisciplinary approaches tailored to the disease stage, focusing on biliary drainage techniques, systemic therapies, and their integration into comprehensive care pathways for PDAC. The continuous refinement of these strategies offers incremental survival benefits and underscores the importance of personalized, multidisciplinary management.
Adverse Outcomes in Neonates Following Planned Home Births: A Case Report Series and a Narrative Literature Review
Authors: Bellini Tommaso , Vinci Francesco , Polleri Giulia , Pezzotta Federico , Grasselli Luca , Piccotti Emanuela , Ramenghi Luca Antonio , Moscatelli Andrea , Striano Pasquale
Published: February 2025

Background: Although home births provide personal and intimate experiences, they pose potential risks that may be better managed in hospital settings. The safety of home birth remains highly debated, with no consensus on its safety or potential adverse events, and its adoption varies widely across the world. In Italy, the Italian Society of Neonatology opposed this practice, resulting in one of the lowest home birth rates in Europe (approximately 0.1% of total births). This study evaluated the impact of planned home births on neonatal health, with a focus on severe complications requiring intervention in the pediatric emergency department (PED). Methods: Cases were collected from patients admitted to IRCCS Istituto Giannina Gaslini Children’s Hospital between January 2022 and December 2024. The analysis focused on neonates born at home, who required emergency care for life-threatening conditions. Results: We identified five cases, with an incidence of approximately 0.65 per 10,000 PED visits and a complication rate of 15–30% for all planned home births. Factors contributing to unfavorable outcomes include lack of advanced medical equipment, timely diagnostics, and comprehensive postpartum screening. Thus, while proponents argue that home births can provide a more comfortable and personalized experience, concerns about safety and associated risks persist. Conclusions: This study aims to highlight the necessity of adopting hospital-level neonatal care protocols for home births, particularly during the critical first 48 h of life, to mitigate risks and ensure optimal neonatal outcomes.
COVID-19 Related Retinal Vascular Occlusion: A Systematic Review
Authors: Tzamalis Argyrios , Foti Maria , Georgiadou Maria , Tsaftaridis Nikolaos , Ziakas Nikolaos
Published: February 2025

Background/Objectives: To provide insight into populations at higher risk of COVID-19-related retinal vascular occlusion, we identified the baseline characteristics of COVID-19 patients and vaccine recipients who developed this condition by conducting a systematic review to summarize the findings and evaluate the current knowledge on this subject. Methods: An electronic search on the PubMed and Scopus databases was performed for relevant case reports or series regarding retinal vascular occlusion in patients with past or present COVID-19 infection or SARS-CoV-2 immunization. This study was conducted using a pre-determined protocol following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results: A total of 34 studies were enrolled in this systematic review. A total of 21 patients (14 males, 66.7%) have been diagnosed with COVID-19 related retinal vein occlusion (RVO, mean age = 41.9 ± 10.3 years), and 15 patients (12 males, 80%) have been diagnosed with retinal artery occlusion (RAO, mean age = 56.9 ± 13.2 years). The time to RVO since COVID-19 infection or SARS-CoV-2 immunization ranged from 8 h to 51 days (mean = 12.3 ± 15.7 days), while the time to RAO ranged from 2 to 40 days (mean = 14.9 ± 10.8 days). Fifteen out of the twenty-one patients (71.4%) with RVO had a significant improvement in visual acuity after the resolution of symptoms while eight out of the fifteen patients (53.3%) with RAO did not show improvement. Conclusions: COVID-19 seems to play a significant role in the pathogenesis of vascular occlusion, as it is suggested to increase the risk of thromboembolic episodes. However, the pathophysiologic mechanisms have not been fully elucidated, and further studies are expected to shed light on this phenomenon.
Mechanical Stability and Clinical Outcomes Following Posterior Cervical Fusion Surgery Using C3-6 Lateral Mass Screw Fixation: En Bloc Versus Regional Screw Fixation
Authors: Lee Dong-Ho , Seok Sang Yun , Lee Woon Sang , Lee Hyung Rae , Park Sehan , Hwang Chang Ju , Cho Jae Hwan
Published: February 2025

Background/Objectives: Although lateral mass screws lower the risk of vertebral artery injury, they are shorter and have a weaker purchase than a pedicle screw, thereby posing the risk of mechanical failure following a ≥3-level posterior cervical fusion (PCF). Therefore, the purpose of this study is to demonstrate that the posterior en bloc fusion of C2-7 is mechanically stronger than shorter, regionally confined posterior fusions of the cervical spine. Methods: We included 178 patients who underwent PCF with ≥3 levels. Patients who underwent PCF that included both C2 and C7 were classified as the en bloc fusion group (EBF group, n = 116), while PCF cases not including these levels were assigned to a regional fusion group (RF group, n = 62). The fusion rate, incidences of mechanical failure, and clinical outcomes were evaluated using univariate analysis between the two groups. Results: The fusion rate was significantly higher in the EBF group than in the RF group (p = 0.038). In contrast, the mechanical failure rate was significantly lower in the EBF group (8/116 [6.9%] vs. 16/62 [25.8%], p = 0.047). Although the ROM was significantly higher in the RF group (p < 0.001), the functional scores did not significantly differ between the two groups. Conclusions: EBF seems to lower the rate of mechanical failure, as well as similar clinical outcomes, compared to RF. When the possibility of mechanical failure is high after PCF, extending the fusion level to C2 and C7 could be considered to minimize mechanical failure, rather than stopping at C3 or C6.
SAVE: Self-Attention on Visual Embedding for Zero-Shot Generic Object Counting
Authors: Zgaren Ahmed , Bouachir Wassim , Bouguila Nizar
Published: February 2025

Zero-shot counting is a subcategory of Generic Visual Object Counting, which aims to count objects from an arbitrary class in a given image. While few-shot counting relies on delivering exemplars to the model to count similar class objects, zero-shot counting automates the operation for faster processing. This paper proposes a fully automated zero-shot method outperforming both zero-shot and few-shot methods. By exploiting feature maps from a pre-trained detection-based backbone, we introduce a new Visual Embedding Module designed to generate semantic embeddings within object contextual information. These embeddings are then fed to a Self-Attention Matching Module to generate an encoded representation for the head counter. Our proposed method has outperformed recent zero-shot approaches, achieving the best Mean Absolute Error (MAE) and Root Mean Square Error (RMSE) results of 8.89 and 35.83, respectively, on the FSC147 dataset. Additionally, our method demonstrates competitive performance compared to few-shot methods, advancing the capabilities of visual object counting in various industrial applications such as tree counting, wildlife animal counting, and medical applications like blood cell counting.
CFD Design Optimisation for the Hydrodynamic Performance of the Novel Fin-Ring Horizontal Axis Hydrokinetic Turbine
Authors: Ibrahim Mahmoud I. , Legaz María J. , Banawan Adel A. , Ahmed Tamer M.
Published: February 2025

In this paper, the aim is to optimise the hydrodynamic performance of the novel fin-ring horizontal axis hydrokinetic turbine (HAHK). The original unique fin-ring turbine is an unconventional marine current turbine that comprises seven concentric rings with 88 connecting cambered fins and a solid centre hub. To begin with, the hydrodynamic performance of the benchmark turbine is evaluated using CFD simulations and is validated against sea-test data available in the literature. Subsequently, three of the turbine design parameters, namely, the fins’ pitch angle, the fins’ camber length, and the fins’ aspect ratio, are optimised for maximum power generation. Further test simulations illustrated the existence of a laminar region of flow in the turbine flow field. The K-kL-ω transition-sensitive turbulence model is adopted to capture the influence of transition on the flow field with results compared against those of the fully turbulent K-ε turbulence model. A final fine-tuning in the turbine design is carried out by increasing the number of fins per ring in the outermost rings to further maximise the generated power. The turbine hydrodynamic performance is assessed by comparison against other conventional hydrokinetic turbines available in the literature. Very satisfactory results are obtained with an increase of about 35% in the turbine-generated CP as compared to that of the benchmark turbine. The turbine performance compares very well with other conventional turbines, especially in terms of higher peak CP values, wider operating TSR range, and less sensitivity to variations in the inflow current speeds.
Enhancement Strategy for Port Resilience: Shipping Route Optimization Methods Based on Network Characteristics of Ports
Authors: Yuan Xiang , He Xinhao
Published: February 2025

Ports and their affiliated shipping routes are fundamental to the maritime logistics system, crucial for global trade. However, they face risks from natural disasters and human-induced crises. Enhancing port resilience, the ability to quickly recover and maintain operations during disruptions is vital for a robust maritime network. This study focuses on enhancing port resilience by improving the shipping route network, using an innovative link-prediction-based approach. Initially, a multi-dimensional resilience analysis is conducted to identify key low-resilience and bottleneck ports, guiding targeted network optimizations. Then, a novel link prediction algorithm is applied to find potential new shipping connections, significantly enhancing network efficiency, robustness, and port resilience. The optimized network effectively improves the connectivity of critical low-resilience ports with central hub ports and bottleneck ports with surrounding ones. Route diversification mitigates risks and strengthens overall resilience. Key low-resilience ports and bottleneck ports are reduced by an average of 20% and 25%. Finally, practical strategies are proposed. Low-resilience ports should establish direct connections with major hubs, and regional sub-networks can offer support. For bottleneck ports, additional secondary and short distance links should be added to transform them into more integrated hubs, enhancing the network’s robustness. These strategies improve the network’s operational capacity during crises, ensuring efficient cargo flow.
Analysis of Interference Magnetic Field Characteristics of Underwater Gliders
Authors: Xie Taotao , Xiao Dawei , Zhang Jiawei , Ji Qing
Published: February 2025

Underwater gliders are a new type of unmanned underwater vehicle, characterized by high energy efficiency, long endurance, and low operational costs. They hold broad application prospects in fields such as ocean exploration, resource surveying, maritime surveillance, and military defense. This paper takes underwater gliders as the research subject, analyzing the characteristics of magnetic interference signals under different operational conditions. The study found that during full operational states, the motor’s operation generates interference signals at 17 Hz; during attitude adjustment, the movement of the moving block generates significant interference magnetic fields, especially during the forward and backward motion of the block, where interference signals at 20 Hz are particularly pronounced. To meet the objective of equipping underwater gliders with magnetic field sensors for underwater target detection, this paper proposes an adaptive filtering method based on the Recursive Least Squares (RLS) algorithm. The experimental results indicate that after filtering with the RLS algorithm, the amplitude of the noise signal has been reduced by over 60%, and it can effectively eliminate the noise components at 17 Hz and 20 Hz caused by the glider’s motor. This algorithm achieves an average increase in the signal-to-noise ratio (SNR) of 12 dB, which is equivalent to an approximately 80% improvement in accuracy. It significantly enhances the stability and signal-to-noise ratio of the magnetic field signals of underwater targets. This provides a feasible solution for equipping underwater gliders with magnetic field sensors for underwater target detection, holding important practical engineering significance.
Coexistence of Built Areas and Natural Protected Areas Within Metropolitan Regions in China
Authors: Yuan Lin , Wang Siqi , Hou Jingxuan
Published: February 2025

Compared with research on the rapid development of Chinese metropolitan regions, systematic studies on nature conservation in metropolitan regions are lacking. We still lack a deep understanding of the spatial relationship between natural protected areas and built-up areas in Chinese metropolitan regions. In this study, we selected four time points, 1990, 2000, 2010, and 2019, to analyze the evolution of the spatial patterns of natural protected areas and built-up areas in metropolitan regions with a population exceeding one million in China, and to analyze their coexistence status. A comprehensive indicator “Index of coexistence degree” (ICD) was established to evaluate the spatial coexistence relationship between natural protected areas and built-up areas, objectively quantifying this coexistence phenomenon. The study found that over the past 30 years, the coexistence of natural protected areas and built-up areas in China’s metropolitan regions has increased significantly: The number of natural protected areas in metropolitan regions has increased by 36 times, the area has increased by 12 times, the nearest distance between natural protected areas and built-up areas has decreased from 15 km to 0.7 km; The ICD value has increased from 0.012 in 1990 to 0.037 in 2019, and the number of cities with high ICD values increased from 1 to 32. Metropolitan regions in China should emphasize the development of natural protected areas in future, spatial pattern for better coexistence with nature is important for biodiversity and human well-being.
Opportunities and Limitations of Urban Agendas as Tools for Sustainable Management of Urban University Campuses
Authors: Rodrigo-Torromé Guillermo , Coronado José M. , Ruiz Rita , Gallego Inmaculada
Published: February 2025

Despite the fact that university campuses have great potential to contribute significantly to urban sustainability, to date, no strategic sustainability plans in this context have been part of any urban agenda, even though these can constitute an adequate framework for such plans. The aim of this paper is to define a procedure for adapting the strategic framework of a national urban agenda, in this case, the Spanish one, to the context of a university, and to discuss the possible limitations and opportunities of this approach. For this purpose, both the strategic framework of the Spanish Urban Agenda (i.e., the strategic and specific objectives and their lines of action) and its sets of indicators (i.e., indicators for the evaluation and monitoring of the objectives and descriptive data) are transferred to the academic context. This procedure makes it possible to obtain a “university urban agenda” with a simplified strategic framework and indicators that are appropriate to the context of universities and that facilitate the collaboration and participation of different agents of the university community to achieve campus management in accordance with the principles of sustainable development.
Optical Coherence Tomography Biomarkers Predict the Long-Term Restorative Effect of Early Anti-VEGF Treatment on Diabetic Macular Edema
Authors: Okudan Süleyman , Acar Duyan Sule , Erdem Abdullah , Bozkurt Oflaz Ayse , Turgut Ozturk Banu
Published: February 2025

Background/Purpose: This study compared the effects of three induction doses of anti-vascular endothelial growth factor (anti-VEGF) on diabetic macular edema (DME) with that of long-term treatment using biomarkers to find out the predictability potential of early response to anti-VEGF treatment for the long-term restorative effect. Methods: We retrospectively reviewed the clinical and optical coherence tomography (OCT) data of 71 DME eyes treated with three monthly anti-VEGF doses and followed for 1 year. BCVA, central subfield thickness (CST), subretinal fluid (SRF), intraretinal cysts, hyperreflective foci (HF), disorganization of inner retinal layers (DRILs), ellipsoid zone/external limiting membrane (EZ/ELM) integrity, and vitreoretinal relationships were assessed at baseline, 3, 6, and 12 months. Results: Patients (50.7% male) had a mean follow-up of 12 months. After three anti-VEGF doses, 19 eyes required no additional injections, 25 continued anti-VEGF, 20 switched to dexamethasone implants, and seven received combination therapy. Best corrected visual acuity (BCVA) improved from 0.52 to 0.40 logMAR at 3 months, 0.30 at 6 months, and stabilized at 0.40 at 12 months. CST decreased from 406 µm to 317 µm at 3 months and 307 µm at 12 months. Significant early improvements in BCVA, CST, SRF, and intraretinal cysts were sustained in the long-term follow-up. HF reduction became significant after 6 months, while DRIL and EZ/ELM integrity remained unchanged. Conclusions: The improvement of OCT biomarkers in DME patients supported that intravitreal anti-VEGF significantly restored the retinal microstructure, which was already evident at 3 months in the control after anti-VEGF induction.
Breaking Barriers to an HIV-1 Cure: Innovations in Gene Editing, Immune Modulation, and Reservoir Eradication
Authors: Borrajo Ana
Published: February 2025

Recent advances in virology, particularly in the study of HIV-1, have significantly progressed the pursuit of a definitive cure for the disease. Emerging therapeutic strategies encompass innovative gene-editing technologies, immune-modulatory interventions, and next-generation antiretroviral agents. Efforts to eliminate or control viral reservoirs have also gained momentum, with the aim of achieving durable viral remission without the continuous requirement for antiretroviral therapy. Despite these promising developments, critical challenges persist in bridging the gap between laboratory findings and clinical implementation. This review provides a comprehensive analysis of recent breakthroughs, ongoing clinical trials, and the barriers that must be addressed to translate these advancements into effective treatments, emphasizing the multifaceted approaches being pursued to achieve a curative solution for HIV-1 infection.
Irritable Bowel Syndrome: A Hallmark of Psychological Distress in Women?
Authors: Marano Giuseppe , Traversi Gianandrea , Pola Roberto , Gasbarrini Antonio , Gaetani Eleonora , Mazza Marianna
Published: February 2025

Irritable bowel syndrome (IBS) is a functional gastrointestinal disorder characterized by abdominal pain, bloating, and altered bowel habits. Women are disproportionately affected by IBS due to a complex interplay between genetic, environmental, and psychosocial factors, along with a crucial role of the gut–brain axis in modulating both bowel function and pain perception. Evidence suggests a strong association between psychological distress and IBS symptoms. Women with IBS report higher levels of psychological distress compared to men, and sex is a biological variable that shapes several aspects of the mechanisms, epidemiology, and clinical manifestations of IBS. This paper explores the bidirectional relationship between psychological factors and IBS with a focus on women. Stress, anxiety, depression, and childhood trauma contribute to IBS symptomatology, and societal and biological factors unique to women may exacerbate this condition. Strategies for integrated care approaches and gender-specific treatment strategies to improve patient outcomes and quality of life are needed.
Exploring Lymphangioma: A Synthesis of the Literature and Clinical Perspectives
Authors: Bochor Nada , Gera Parshotam
Published: February 2025

Lymphatic malformations (LMs) are benign, congenital vascular anomalies caused by abnormal lymphangiogenesis during embryology, often presenting as fluid-filled cystic lesions. Though LMs can affect any part of the body except the brain, they primarily manifest in the head and neck or axilla regions of children. With a prevalence of approximately 1 in 4000 births, LMs are commonly diagnosed by age two, with symptoms varying based on lesion location and size. This paper reviews the classification of LMs and discusses the de Serres staging system, which aids in assessing prognosis based on lesion site. Mutations in the (PIK3CA) gene are implicated in most cases, and LMs are also associated with syndromic conditions like Turner and Noonan syndromes. They are diagnosed by ultrasound (USS) or magnetic resonance imaging (MRI), while a histologic analysis can confirm lymphatic origin. Treatment options range from conservative approaches, such as observation, to sclerotherapy, pharmacotherapy, and surgery. Sclerotherapy, particularly with agents like OK-432, bleomycin, and doxycycline, has shown significant efficacy in reducing LM size and symptoms with minimal side effects. Pharmacological therapies, such as sirolimus, that target the mTOR pathway are also increasingly being used, with a good effect on the burden of disease. While surgical excision remains a choice for symptomatic or large lesions, minimally invasive approaches are often preferred due to lower morbidity. Emerging techniques include gravity-dependent sclerotherapy, electrosclerotherapy, alpelisib, everolimus, and Wnt/β-catenin pathway stimulators (e.g., tankyrase inhibitors, porcupine inhibitors). Computational atomistic molecular dynamics (MD) and density functional tight binding (DFTB) techniques may offer an experimental approach to future therapeutic targets. This paper highlights a multidisciplinary approach to LM management, emphasising individualised treatment based on lesion characteristics and patient needs.
Magnetic Anisotropy and Slow Magnetic Relaxation in Two Mononuclear Octahedral Cobalt(II) Complexes
Authors: Cui Hui-Hui , Wang Dou-Zun , Li Shixiang , Wang Leixin , Yu Xinrui , Liu Xiancong , Wang Jin , Wang Miao , Tang Yanfeng
Published: February 2025

Two mononuclear octahedral Co(II) complexes, [Co(L)X2] (L = 1-(prop-2-en-1-yl)-1H-imidazole, X = NCS− (1) and NCSe− (2)), have been synthesized and characterized. The central Co(II) ions in two complexes adopt an octahedral geometry, coordinated by four N atoms from the ligand and two N atoms from the anion. Direct-current magnetic data revealed large easy-plane magnetic anisotropy in both 1 and 2. Dynamic magnetic measurements demonstrated that 1 and 2 display field-induced slow magnetic relaxation. For 1 and 2, the Raman mechanism is found to the dominant process in the whole temperature range. Compared to 1, the magnetic relaxation of 2 is faster, likely due to the presence of the hydrogen bonding system in 2.
Different Susceptibilities of Wheat Straw and Corn Stover to Mechanical Pretreatment for Biomethane Production
Authors: Dell’Omo Pier Paolo
Published: February 2025

The effects of milling on the anaerobic degradability of wheat straw and corn stover were investigated. Pretreatment was carried out by an industrial-scale device, able to process over one ton per hour. After 28 days of digestion under mesophilic conditions, the cumulative methane production from the pretreated straw (250 Nm3 t−1 of volatile solids) was 49.2% greater than that from the raw material. Pretreated stover reached a cumulative methane yield of 219.8 Nm3 t−1 of volatile solids, gaining 10.1% as compared to the feedstock. The specific electrical energy requirements for pretreatment were 66.6 kWh t−1 for processed straw and 64.8 kWh t−1 for stover; these consumptions were not significantly different. With reference to biomethane production, the impact of raw material on the production cost decreased from EUR 0.418 Nm−3 to EUR 0.328 Nm−3 for pretreated straw, whereas it increased by 5.8% for corn stover, whose pretreatment, therefore, was not economically feasible.
High-Performance Ag-NWs Doped Graphene/ITO Hybrid Transparent Conductive Electrode
Authors: Bourahla Hana , Fernández Susana , Ryu Yu Kyoung , Velasco Andres , Malkia Chahinez , Boscá Alberto , Gómez-Mancebo M. Belén , Calle Fernando , Martinez Javier
Published: February 2025

Indium tin oxide (ITO) is a commonly used material for transparent conductive electrodes (TCE) in optoelectronic applications. On the other hand, graphene has superior electrical conductivity and exceptional mechanical flexibility, which makes it a promising candidate as a TCE material. This work proposes a CVD graphene/ITO hybrid electrode enhanced by doping with silver nanowires (Ag-NWs). The study aims to improve the performance of the electrode by optimizing two key parameters during the fabrication process: the thermal annealing time after the transfer of graphene on ITO and the Ag-NWs doping conditions. The annealing treatment is fundamental to reducing the residues on the surface of graphene and increasing the interface contact between graphene and ITO. The correct coverage and distribution of the dopant on graphene is obtained by controlling the concentration of the Ag-NWs and the spin coating speeds. The results indicate a substantial improvement in the optical and electrical performance of the Ag-NWs/graphene/ITO hybrid electrode. A remarkably low sheet resistance of 42.4 Ω/sq (±2 Ω/sq) has been achieved while maintaining a high optical transmittance of 87.3% (±0.5%).
Uncovering the Multifaceted Role of PA2649 (nuoN) in Type III Secretion System and Other Virulence Production in Pseudomonas aeruginosa PAO1
Authors: Chen Lin , Si Yujie , Han Xue , Xiao Yue , Pan Yidan , Duan Kangmin , Fu Songzhe
Published: February 2025

Pseudomonas aeruginosa is a multi-drug-resistant opportunistic pathogen that adapts to challenging environments by deploying virulence factors, including the type III secretion system (T3SS). Emerging evidence points to a role for NADH dehydrogenase complexes in regulating virulence; however, their precise contributions remain unclear. Here, we identify PA2649, a component of the NADH dehydrogenase complex I (nuo operon), as a key regulator of T3SS-related activities. PA2649 deletion resulted in a twofold increase in exoS expression and enhanced cytotoxicity in both A549 cell and Chinese cabbage models. Full revertant of the nuo operon was necessary to restore exoS expression to wild-type levels, suggesting a critical connection between NADH dehydrogenase activity and T3SS regulation. The PA2649 mutation also disrupted the Rsm-Exs regulatory axis, downregulating gacS, rsmY, rsmZ, and hfq while upregulating exsC. Overexpression of rsmY, rsmZ, gacA, hfq, and exsD partially rescued T3SS function, confirming that PA2649 influences T3SS via the Rsm-Exs pathway. Furthermore, PA2649 deletion altered motility, biofilm formation, pyocyanin production, protease activity, and antibiotic susceptibility. These phenotypes could not be complemented with T3SS regulatory genes alone, indicating that PA2649 modulates these traits through mechanisms independent of the Rsm-Exs axis, potentially involving NADH dehydrogenase-associated pathways. This study underscores the multifaceted role of PA2649 in regulating P. aeruginosa pathogenicity and resistance, providing novel insights into its complex regulatory networks and highlighting new avenues for therapeutic targeting.
Antischistosomal Potential of Animal-Derived Natural Products and Compounds
Authors: Fischer-Carvalho Agatha , Taveira-Barbosa Tereza Cristina , Verjovski-Almeida Sergio , Haeberlein Simone , Sena Amaral Murilo
Published: February 2025

Schistosomiasis is a neglected tropical disease that affects over 240 million people worldwide. Currently, praziquantel is the only drug recommended by the World Health Organization for treatment. However, cases of drug resistance have been reported, which indicates an urgent need for new therapeutics. In this context, natural compounds represent valuable sources of pharmacological substances. Plant-derived natural products have been greatly explored for their potential antischistosomal activity, while animal-derived compounds have received little attention. Recent advances in the biotechnology field allow the wide exploration of animal-derived compounds in drug discovery, which may represent a cost-effective option to find bioactive molecules also against Schistosoma mansoni and other parasites. This review highlights the research into animal-derived products and compounds that have already been tested against schistosomes. Phenotypic effects on schistosomes have been observed upon incubation with some of these substances, which may, therefore, represent possible candidates to be used in the development of new drugs. Overall, these studies advance the discovery of antischistosomal compounds by exploring a yet understudied natural resource. The present review also discusses the challenges of testing animal-derived products and provides examples of the experimental in vitro testing of different selected animal natural products against S. mansoni.
Selective Separation of La(III) and Ce(III) Using Hollow Fiber Membranes: Influence of pH and Extractant Systems
Authors: Olea Felipe , Ulloa Laura , Bringas Eugenio , Urzúa-Ahumada Julio , Abejón Ricardo , Romero Julio , Quijada-Maldonado Esteban
Published: February 2025

The selective separation of adjacent rare earth elements (REEs), such as La(III) and Ce(III), is a critical challenge in hydrometallurgy due to their similar chemical properties. This work evaluates the performance of non-dispersive solvent extraction (NDSX) using hollow fiber (HF) membranes for this purpose. Initial solvent extraction (SX) equilibrium experiments with Cyanex® 272 in kerosene determined that the aqueous phase’s optimal pH for selectivity is 5.6, achieving a selectivity of αCe/La=12.7. NDSX experiments demonstrated enhanced selectivity αCe/La=34 after 120 min, benefiting from the additional mass transfer resistance provided by the HF membrane. Maintaining a constant pH of 5.0 with NaOH improved extraction rates but slightly reduced selectivity to αCe/La=26. Experiments using 1,1,1-trifluoro-2,4-pentanedione (HTFAC) in the ionic liquid (IL) [Omim][Tf2n] as the receiving phase showed lower extraction rates but achieved comparable selectivity values (αCe/La=22) in just 20 min, thanks to the IL’s viscosity limiting La(III) extraction. The impact of HF membrane design was also assessed; increasing the membrane’s surface area significantly improved extraction rates but reduced selectivity due to reduced mass transfer resistance. These results demonstrate the potential of NDSX systems for selective REE separation, particularly by leveraging controlled mass transfer and operating conditions. However, further work is needed to optimize system design. The findings highlight the advantages of NDSX over traditional SX, offering a promising pathway for sustainable and efficient REE processing.
Effect of Curcumin Pretreatment on the Susceptibility of Cryptococcus neoformans to Photodynamic Therapy Mediated by Aluminum Phthalocyanine in Nanoemulsion
Authors: Costa Fabiana Chagas , Nunes Lourival Carvalho , Ranjan Kunal , Silveira Ariane Pandolfo , Martins da Silva Ingrid Gracielle , Mariano André de Lima e Silva , de Souza Paulo Eduardo Narcizo , Báo Sônia Nair , Poças-Fonseca Marcio Jose , Muehlmann Luis Alexandre
Published: February 2025

Background/Objectives: Curcumin has antimicrobial activity, and its mechanism of action involves changing histone acetylation. Our group has shown that histone deacetylases (HDACs) inhibitors increase the sensibility of Cryptococcus neoformans to certain antifungal treatments. Therefore, the aim of this work was to investigate whether curcumin pretreatment increases the effect of photodynamic therapy (PDT) mediated by aluminum phthalocyanine in nanoemulsion (AlPc-NE) against C. neoformans. Methods: The minimum inhibitory concentrations (MIC) of AlPc-NE and curcumin, along with the 72-h growth curve of cells exposed to the combined treatments, were evaluated in the C. neoformans reference strain H99. Additionally, further analysis was performed using HDAC gene deletion mutant strains, hda1Δ and hos2Δ. Results: Curcumin reduces the effect of PDT on C. neoformans reference strain H99, likely due to its antioxidant properties. In the hda1Δ strain, 50% MIC of curcumin reduced the effect of PDT, but this effect was not observed in response to 75% MIC of curcumin. Conversely, in the hos2Δ strain, pretreatment with curcumin at 75% MIC enhanced the efficacy of PDT in combination with 50% MIC of AlPc-NE. Conclusions: These results indicate that curcumin inhibits C. neoformans. Moreover, at lower concentrations, curcumin protects cells against oxidant damage, while at higher concentrations, it may trigger epigenetic mechanisms that compromise cell viability. In conclusion, both curcumin and PDT are active against C. neoformans, with HDACs affecting their efficacy, and the effectiveness of the combined treatment depends on the concentration of both curcumin and AlPc-NE.
Agave macroacantha Transcriptome Reveals Candidate CNGC Genes Responsive to Cold Stress in Agave
Authors: Li Yubo , Hu Xiaoli , Mkapa Dietram Samson , Xie Li , Guo Pingan , Tan Shibei , Zhang Weiyi , Chen Helong , Huang Xing , Yi Kexian
Published: February 2025

Agave, with its unique appearance and ability to produce hard fibers, holds high economic value. However, low temperatures during winter can restrict its growth and even damage the leaves, causing a loss of ornamental appeal or affecting the fiber quality. Conversely, the plant cyclic nucleotide-gated channel (CNGC) family plays an important role in the growth and development of plants and the response to stress. Studying the CNGC family genes is of great importance for analyzing the mechanism by which agave responds to cold stress. This research conducted a transcriptomic analysis of the ornamental plant Agave macroacantha. Through assembly via Illumina sequencing, 119,911 transcripts were obtained, including 78,083 unigenes. In total, 6, 10, 11, and 13 CNGC genes were successfully identified from A. macroacantha, Agave. H11648, Agave. deserti, and Agave. tequilana, respectively. These CNGC genes could be divided into four groups (I, II, III, and IV), and group IV could be divided into two subgroups (IV-A and IV-B). The relative expression levels were quantified by qRT-PCR assays, which revealed that AhCNGC4.1 was significantly upregulated after cold treatment and Ca(NO3)2 treatment, suggesting its importance in cold stress and calcium signaling. Additionally, the Y2H assay has preliminarily identified interacting proteins of AhCNGC4.1, including AhCML19 and AhCBSX3. This study has established a completely new transcriptome dataset of A. macroacantha for the first time, enriching the bioinformatics of agave’s transcriptome. The identified CNGC genes are of great significance for understanding the evolution of agave species. The cloned CNGC genes, expression pattern analysis, and protein interaction results laid a foundation for future research related to the molecular functions of agave CNGC genes in cold tolerance.