bluuebunny/crossref_metadata_2025 huggingface dataset:
Research examining a spatial autocorrelation imaging method based on stationary characteristics of microtremors
Authors: Du Qingling , Pan Yanhui , Zhao Kuanyao , Gao Denghui
Published: February 2024

The spatial autocorrelation method is an important method for extracting the velocity dispersion curve from microtremor data. However, site data typically cannot strictly meet spatial and temporal stationary feature, and this greatly affects the accuracy of the calculation results of this method. Therefore, based on the cosine similarity theory, this study deduces the applicability of the spatial autocorrelation method to unidirectional Rayleigh surface waves and again verifies the applicability of this method to spatially and temporally stationary Rayleigh waves. The numerical simulation results demonstrate that the velocity dispersion curve can be extracted from a one-way Rayleigh wave using the spatial autocorrelation method to obtain an accurate geological profile, whereas the superposition of finite groups of Rayleigh waves in different directions cannot yield an accurate geological profile. In this study, we quantitatively analyzed the impact of the spatial autocorrelation method on the extraction of the velocity dispersion curve when the signal could not meet the characteristics of temporal and spatial stationarity through numerical simulation. The results reveal that the velocity-dispersion curve can be accurately extracted only when the signal satisfies both spatial and temporal stationarity. When a signal is closer to the spatial and temporal stationary characteristics, this indicates that a more accurate velocity dispersion curve can be extracted. These results provide a reference for improving the calculation accuracy of spatial autocorrelation methods.
Adaptive auditory assistance for stride length cadence modification in older adults and people with Parkinson’s
Authors: Wu Tina L. Y. , Murphy Anna , Chen Chao , Kulić Dana
Published: February 2024

Gait rehabilitation using auditory cues can help older adults and people with Parkinson’s improve walking performance. While auditory cues are convenient and can reliably modify gait cadence, it is not clear if auditory cues can reliably modify stride length (SL), another key gait performance metric. Existing algorithms also do not address habituation or fluctuation in motor capability, and have not been evaluated with target populations or under dual-task conditions. In this study, we develop an adaptive auditory cueing framework that aims to modulate SL and cadence. The framework monitors the gait parameters and learns a personalized cue-response model to relate the gait parameters to the input cues. The cue-response model is represented using a multi-output Gaussian Process (MOGP) and is used during optimization to select the cue to provide. The adaptive cueing approach is benchmarked against the fixed approach, where cues are provided at a fixed cadence. The two approaches are tested under single and dual-task conditions with 13 older adults (OA) and 8 people with Parkinson’s (PwP). The results show that more than half of the OA and PwP in the study can change both SL and cadence using auditory cues. The fixed approach is best at changing people’s gait without secondary task, however, the addition of the secondary task significantly degrades effectiveness at changing SL. The adaptive approach can maintain the same level of SL change regardless of the presence of the secondary task. A separate analysis is conducted to identify factors that influence the performance of the adaptive framework. Gait information from the previous time step, along with the previous input cue, can improve its prediction accuracy. More diversity in the initialization data can also improve the GP model. Finally, we did not find a strong correlation between stride length and cadence when the parameters are contingent upon input cues.
A novel computational framework for the estimation of internal musculoskeletal loading and muscle adaptation in hypogravity
Authors: Cowburn James , Serrancolí Gil , Pavei Gaspare , Minetti Alberto , Salo Aki , Colyer Steffi , Cazzola Dario
Published: February 2024

Introduction: Spaceflight is associated with substantial and variable musculoskeletal (MSK) adaptations. Characterisation of muscle and joint loading profiles can provide key information to better align exercise prescription to astronaut MSK adaptations upon return-to-Earth. A case-study is presented of single-leg hopping in hypogravity to demonstrate the additional benefit computational MSK modelling has when estimating lower-limb MSK loading.

Methods: A single male participant performed single-leg vertical hopping whilst attached to a body weight support system to replicate five gravity conditions (0.17, 0.25, 0.37, 0.50, 1 g). Experimental joint kinematics, joint kinetics and ground reaction forces were tracked in a data-tracking direct collocation simulation framework. Ground reaction forces, sagittal plane hip, knee and ankle net joint moments, quadriceps muscle forces (Rectus Femoris and three Vasti muscles), and hip, knee and ankle joint reaction forces were extracted for analysis. Estimated quadriceps muscle forces were input into a muscle adaptation model to predict a meaningful increase in muscle cross-sectional area, defined in (DeFreitas et al., 2011).

Results: Two distinct strategies were observed to cope with the increase in ground reaction forces as gravity increased. Hypogravity was associated with an ankle dominant strategy with increased range of motion and net plantarflexor moment that was not seen at the hip or knee, and the Rectus Femoris being the primary contributor to quadriceps muscle force. At 1 g, all three joints had increased range of motion and net extensor moments relative to 0.50 g, with the Vasti muscles becoming the main muscles contributing to quadriceps muscle force. Additionally, hip joint reaction force did not increase substantially as gravity increased, whereas the other two joints increased monotonically with gravity. The predicted volume of exercise needed to counteract muscle adaptations decreased substantially with gravity. Despite the ankle dominant strategy in hypogravity, the loading on the knee muscles and joint also increased, demonstrating this provided more information about MSK loading.

Discussion: This approach, supplemented with muscle-adaptation models, can be used to compare MSK loading between exercises to enhance astronaut exercise prescription.
Direct modulation of TRPC ion channels by Gα proteins
Authors: Kang Hana , Kim Jinhyeong , Park Christine Haewon , Jeong Byeongseok , So Insuk
Published: February 2024

GPCR-Gi protein pathways are involved in the regulation of vagus muscarinic pathway under physiological conditions and are closely associated with the regulation of internal visceral organs. The muscarinic receptor-operated cationic channel is important in GPCR-Gi protein signal transduction as it decreases heart rate and increases GI rhythm frequency. In the SA node of the heart, acetylcholine binds to the M2 receptor and the released Gβγ activates GIRK (I(K,ACh)) channel, inducing a negative chronotropic action. In gastric smooth muscle, there are two muscarinic acetylcholine receptor (mAChR) subtypes, M2 and M3. M2 receptor activates the muscarinic receptor-operated nonselective cationic current (mIcat, NSCC(ACh)) and induces positive chronotropic effect. Meanwhile, M3 receptor induces hydrolysis of PIP2 and releases DAG and IP3. This IP3 increases intracellular Ca2+ and then leads to contraction of GI smooth muscles. The activation of mIcat is inhibited by anti-Gi/o protein antibodies in GI smooth muscle, indicating the involvement of Gαi/o protein in the activation of mIcat. TRPC4 channel is a molecular candidate for mIcat and can be directly activated by constitutively active GαiQL proteins. TRPC4 and TRPC5 belong to the same subfamily and both are activated by Gi/o proteins. Initial studies suggested that the binding sites for G protein exist at the rib helix or the CIRB domain of TRPC4/5 channels. However, recent cryo-EM structure showed that IYY58-60 amino acids at ARD of TRPC5 binds with Gi3 protein. Considering the expression of TRPC4/5 in the brain, the direct G protein activation on TRPC4/5 is important in terms of neurophysiology. TRPC4/5 channels are also suggested as a coincidence detector for Gi and Gq pathway as Gq pathway increases intracellular Ca2+ and the increased Ca2+ facilitates the activation of TRPC4/5 channels. More complicated situation would occur when GIRK, KCNQ2/3 (IM) and TRPC4/5 channels are co-activated by stimulation of muscarinic receptors at the acetylcholine-releasing nerve terminals. This review highlights the effects of GPCR-Gi protein pathway, including dopamine, μ-opioid, serotonin, glutamate, GABA, on various oragns, and it emphasizes the importance of considering TRPC4/5 channels as crucial players in the field of neuroscience.
The inhibitory potential of green manure return on the germination and seedling growth of Eleusine indica L.
Authors: Zhang Ying , Liu Silin , Du Xiao , Chen Zhongwen , Ma Zhiyu , Mu Yinghui
Published: February 2024

Trifolium repens L. (white clover) and Lolium perenne L. (ryegrass) are green manures widely used in conservation tillage systems worldwide. Eleusine indica L. (goosegrass) is a globally recognized noxious weed. Herein, we investigated the effects of aqueous extracts, decomposed liquids, and different straw-to-soil ratios on the germination and growth of goosegrass. The results showed that high concentrations (≥ 30%) of aqueous extracts or decomposed liquids of both green manures significantly inhibited germination-related parameters of goosegrass. The strongest inhibitory effect was observed for the 7-day decomposition treatment, and white clover’s inhibitory effect was greater than ryegrass’s. A pot experiment showed that non-photochemical quenching, catalase, and peroxidase activity levels of goosegrass leaves were significantly increased. At the same time, the net photosynthetic rate significantly decreased. Seedling growth was inhibited when the straw-to-soil ratio was greater than 3:100. The ryegrass treatments inhibited goosegrass seedlings more than the white clover treatments. This study demonstrated the inhibitory potential of white clover and ryegrass straw return on seed germination and seedling growth of goosegrass. The study has also helped to identify weed-resistant substances in these green manures so that their weed-control properties can be used more effectively and herbicide usage can be reduced.
Phylogeny, biogeography, and character evolution of Anaphalis (Gnaphalieae, Asteraceae)
Authors: Xu Xue-Min , Xu He , Yang Zheng , Wei Zhen , Gu Jun-Yu , Liu Dan-Hui , Liu Quan-Ru , Zhu Shi-Xin
Published: February 2024

The HAP clade, mainly including Helichrysum Mill, Anaphalis DC., and Pseudognaphalium Kirp., is a major component of tribe Gnaphalieae (Asteraceae). In this clade, Anaphalis represents the largest genus of Asian Gnaphalieae. The intergeneric relationships among Anaphalis and its related genera and the infrageneric taxonomy of this genus are complex and remain controversial. However, there are few studies that have focused on these issues. Herein, based on the current most comprehensive sampling of the HAP clade, especially Anaphalis, we conducted phylogenetic analyses using chloroplast (cp) genome and nuclear ribosomal DNA (nrDNA) to evaluate the relationships within HAP clade, test the monophyly of Anaphalis, and examine the infrageneric taxonomy of this genus. Meanwhile, the morphological characters were verified to determine the circumscription and infrageneric taxonomy system of Anaphalis. Additionally, the biogeographical history, diversification processes, and evolution of crucial morphological characters were estimated and inferred. Our phylogenetic analyses suggested that Anaphalis is polyphyletic because it nested with Helichrysum and Pseudognaphalium. Two and four main clades of Anaphalis were identified in cp genome and nrDNA trees, respectively. Compared with nrDNA trees, the cp genome trees were more effective for phylogenetic resolution. After comprehensively analyzing morphological and phylogenetic evidence, it was concluded that the achene surface ornamentation and leaf base showed less homoplasy and supported the two Anaphalis lineages that were inferred from cp genome. Our biogeographical analyses based on cp genome indicated that HAP clade underwent rapid diversification from late Miocene to Pliocene. The two Anaphalis lineages appeared to have originated in Africa, then spread to Western and Southern Asia, and subsequently moved into Southwestern China forming a diversity center. The dispersal patterns of the two Anaphalis lineages were different. One dispersed around the world, except in Africa and South America. The other one dispersed to Eastern and Southeastern Asia from the ancestral origin region.
More N fertilizer, more maize, and less alfalfa: maize benefits from its higher N uptake per unit root length
Authors: Shao Zeqiang , Zheng Congcong , Postma Johannes Auke , Gao Qiang , Zhang Jinjing
Published: February 2024

Root plasticity is fundamental to soil nutrient acquisition and maximizing production. Different soil nitrogen (N) levels affect root development, aboveground dry matter accumulation, and N uptake. This phenotypic plasticity is well documented for single plants and specific monocultures but is much less understood in intercrops in which species compete for the available nutrients. Consequently, the study tested whether the plasticity of plant roots, biomass and N accumulation under different N levels in maize/alfalfa intercropping systems differs quantitatively. Maize and alfalfa were intercropped for two consecutive years in large soil-filled rhizoboxes and fertilized with 6 different levels of N fertilizer (0, 75, 150, 225, 270, and 300 kg ha-1). Root length, root surface area, specific root length, N uptake and yield were all increased in maize with increasing fertilizer level, whereas higher N rates were supraoptimal. Alfalfa had an optimal N rate of 75-150 kg ha-1, likely because the competition from maize became more severe at higher rates. Maize responded more strongly to the fertilizer treatment in the second year when the alfalfa biomass was much larger. N fertilization contributes more to maize than alfalfa growth via root plasticity responses. Our results suggest that farmers can maximize intercropping yield and economic return by optimizing N fertilizer management.
Few-shot disease recognition algorithm based on supervised contrastive learning
Authors: Mu Jiawei , Feng Quan , Yang Junqi , Zhang Jianhua , Yang Sen
Published: February 2024

Diseases cause crop yield reduction and quality decline, which has a great impact on agricultural production. Plant disease recognition based on computer vision can help farmers quickly and accurately recognize diseases. However, the occurrence of diseases is random and the collection cost is very high. In many cases, the number of disease samples that can be used to train the disease classifier is small. To address this problem, we propose a few-shot disease recognition algorithm that uses supervised contrastive learning. Our algorithm is divided into two phases: supervised contrastive learning and meta-learning. In the first phase, we use a supervised contrastive learning algorithm to train an encoder with strong generalization capabilities using a large number of samples. In the second phase, we treat this encoder as an extractor of plant disease features and adopt the meta-learning training mechanism to accomplish the few-shot disease recognition tasks by training a nearest-centroid classifier based on distance metrics. The experimental results indicate that the proposed method outperforms the other nine popular few-shot learning algorithms as a comparison in the disease recognition accuracy over the public plant disease dataset PlantVillage. In few-shot potato leaf disease recognition tasks in natural scenarios, the accuracy of the model reaches the accuracy of 79.51% with only 30 training images. The experiment also revealed that, in the contrastive learning phase, the combination of different image augmentation operations has a greater impact on model. Furthermore, the introduction of label information in supervised contrastive learning enables our algorithm to still obtain high accuracy in few-shot disease recognition tasks with smaller batch size, thus allowing us to complete the training with less GPU resource compared to traditional contrastive learning.
NO enhances the adaptability to high-salt environments by regulating osmotic balance, antioxidant defense, and ion homeostasis in eelgrass based on transcriptome and metabolome analysis
Authors: Wang Xianyan , Wang Tongtong , Yu Pei , Li Yuchun , Lv Xinfang
Published: February 2024

Introduction

Eelgrass is a typical marine angiosperm that exhibits strong adaptability to high-salt environments. Previous studies have shown that various growth and physiological indicators were significantly affected after the nitrate reductase (NR) pathway for nitric oxide (NO) synthesis in eelgrass was blocked.

Methods

To analyze the molecular mechanism of NO on the adaptability to high-salt environment in eelgrass, we treated eelgrass with artificial seawater (control group) and artificial seawater with 1 mM/L Na2WO4 (experimental group). Based on transcriptomics and metabolomics, we explored the molecular mechanism of NO affecting the salt tolerance of eelgrass.

Results

We obtained 326, 368, and 859 differentially expressed genes (DEGs) by transcriptome sequencing in eelgrass roots, stems, and leaves, respectively. Meanwhile, we obtained 63, 52, and 36 differentially accumulated metabolites (DAMs) by metabolomics in roots, stems, and leaves, respectively. Finally, through the combined analysis of transcriptome and metabolome, we found that the NO regulatory mechanism of roots and leaves of eelgrass is similar to that of terrestrial plants, while the regulatory mechanism of stems has similar and unique features.

Discussion

NO in eelgrass roots regulates osmotic balance and antioxidant defense by affecting genes in transmembrane transport and jasmonic acid-related pathways to improve the adaptability of eelgrass to high-salt environments. NO in eelgrass leaves regulates the downstream antioxidant defense system by affecting the signal transduction of plant hormones. NO in the stems of eelgrass regulates ion homeostasis by affecting genes related to ion homeostasis to enhance the adaptability of eelgrass to high-salt environments. Differently, after the NO synthesis was inhibited, the glyoxylate and dicarboxylate metabolism, as well as the tricarboxylic acid (TCA) cycle, was regulated by glucose metabolism as a complementary effect to cope with the high-salt environment in the stems of eelgrass. These are studies on the regulatory mechanism of NO in eelgrass, providing a theoretical basis for the study of the salt tolerance mechanism of marine plants and the improvement of terrestrial crop traits. The key genes discovered in this study can be applied to increase salt tolerance in terrestrial crops through cloning and molecular breeding methods in the future.
Adapting a self-efficacy scale to the task of teaching scientific reasoning: collecting evidence for its psychometric quality using Rasch measurement
Authors: Welter Virginia Deborah Elaine , Dawborn-Gundlach Merryn , Großmann Leroy , Krell Moritz
Published: February 2024

Besides teachers' professional knowledge, their self-efficacy is a crucial aspect in promoting students' scientific reasoning (SR). However, because no measurement instrument has yet been published that specifically refers to self-efficacy beliefs regarding the task of teaching SR, we adapted the Science Teaching Efficacy Belief Instrument (STEBI) accordingly, resulting in the Teaching Scientific Reasoning Efficacy Beliefs Instrument (TSR-EBI). While the conceptual framework of the TSR-EBI is comparable to that of the STEBI in general terms, it goes beyond it in terms of specificity, acknowledging the fact that teaching SR requires very specific knowledge and skills that are not necessarily needed to the same extent for promoting other competencies in science education. To evaluate the TSR-EBI's psychometric quality, we conducted two rounds of validation. Both samples (N1 = 114; N2 = 74) consisted of pre-service teachers enrolled in university master's programs in Germany. The collected data were analyzed by applying Rasch analysis and known-group comparisons. In the course of an analysis of the TSR-EBI's internal structure, we found a 3-category scale to be superior to a 5-category structure. The person and item reliability of the scale proved to be satisfactory. Furthermore, during the second round of validation, it became clear that the results previously found for the 3-category scale were generally replicable across a new (but comparable) sample, which clearly supports the TSR-EBI's psychometric quality. Moreover, in terms of test-criterion relationships, the scale was also able to discriminate between groups that are assumed to have different levels of self-efficacy regarding teaching SR. Nonetheless, some findings also suggest that the scale might benefit from having the selection of individual items reconsidered (despite acceptable item fit statistics). On balance, however, we believe that the TSR-EBI has the potential to provide valuable insights in future studies regarding factors that influence teachers' self-efficacy, such as their professional experiences, prior training, or perceived barriers to effective teaching.
The effects of mind-body exercise on anxiety and depression in older adults: a systematic review and network meta-analysis
Authors: Dong Yangjian , Zhang Xinxin , Zhao Rongting , Cao Lan , Kuang Xiaoqin , Yao Jiwei
Published: February 2024

Background

Limited research directly compares the clinical effects of different types of mind-body exercises on anxiety and depression in older adults. Therefore, we conducted a systematic review and network meta-analysis of randomized controlled trials that meet the inclusion criteria to explore the intervention effects of five different types of mind-body exercises in improving anxiety and depression in older adults.

Methods

We followed the PRISMA-NMA guidelines and conducted searches in the Web of Science, PubMed, the Cochrane Library, and Embase databases up to July 28, 2023. The language was limited to English. Two independent reviewers conducted literature screening and data extraction. Review Manager 5.4 was used to perform Pairwise meta-analysis and risk assessment, while STATA version 15 software was used for network meta-analysis.

Result

A total of 42 studies, involving 2974 participants, were included. The results of the traditional meta-analysis showed that mind-body exercises were superior to the control group in alleviating anxiety (SMD: -0.87, 95% CI: -1.43, -0.31, p<0.05, I2 = 95%) and depressive (SMD: -0.52, 95% CI: -0.71, -0.34, p<0.05, I2 = 80%). In the network meta-analysis, the ranking of treatment effects for anxiety showed that Tai Chi > Qigong > Yoga > Dance > control group, while for depression, the ranking showed Tai Chi > Pilates > Yoga > Qigong > Dance > control group.

Conclusion

This study found that mind-body exercises have positive effects on improving anxiety and depression in older adults. Among the five different types of mind-body exercise interventions, Tai Chi was considered an effective approach for improving anxiety and depression. However, we encourage older adults to choose exercise modalities that suit their interests to enhance adherence.

Systematic review registration

http://www.crd.york.ac.uk/PROSPERO/, identifier CRD42023464296.
Impact of traditional Chinese Baduanjin exercise on menstrual health among international female students studying in China: a randomized controlled trial
Authors: Alkhatib Asem , Alshikh Ahmad Hoda , Zhang Ci , Peng Wenwen , Li Xianhong
Published: February 2024

Background

Menstrual symptoms were the common complaints of international female students during the acculturation period, and the symptoms negatively affected the daily life and school performance of those women. The present study aimed to evaluate the effectiveness of the traditional Chinese Baduanjin exercise on reducing menstrual symptoms for international female students studying in China during the acculturation period.

Methods

We conducted a randomized controlled trial among 62 international female students who suffered menstrual symptoms during the first 6 months after arriving in China. The study was carried out at three campuses of the two largest universities in the capital of a province in south-central China from March to October 2021. After screening, participants were randomly assigned to a control or intervention group. The intervention group engaged in 24 weeks of Baduanjin exercise for at least 30 min per day. The menstrual symptoms, sociocultural adaptation, perceived stress, and sleep quality were evaluated at baseline, the 12th week, and the 24th week. The chi-square test or Fisher's exact test, paired sample t-test, and multivariate analysis of variance (MANOVA)were adopted to analyze the data using SPSS 25.0.

Results

Totally, 62 participants were enrolled in this study from 30 countries. Their mean age was 27.87 ± 5.58 years. None of the participants reported an adverse event. The results of the MANOVA test showed that the traditional Chinese Baduanjin exercise reduced the total score of MSQ among international female students (intervention: 47.83 ± 8.71 vs. control: 65.12 ± 16.86; F = 19.49, P < 0.01). In addition, the results of the MANOVA indicated statistically significant decreases in scores for the two subscales of MSQ: premenstrual symptoms (intervention: 17.07 ± 3.33 vs. control: 28.42 ± 7.56; F = 44.70, P < 0.01)and menstrual pain (16.03 ± 4.53 vs. 19.58 ± 5.14; F = 6.50, P < 0.05). Moreover, the results showed that traditional Chinese Baduanjin exercise reduced the scores of the sociocultural adaptation level, the perceived stress level, and sleep quality and improved the blood progesterone hormone and blood estrogen hormone.

Conclusion

Regular Baduanjin exercise is a safe, acceptable, and effective form of exercise to promote international female students' menstrual health, reduce stress levels, and improve sleep quality.

Trial registration

www.chictr.org.cn, Chinese Clinical Trial Registry: ChiCTR2300072376.
A first insight into tuberculosis transmission at the border of Ecuador and Colombia: a retrospective study of the population structure of Mycobacterium tuberculosis in Esmeraldas province
Authors: Castro-Rodriguez Bernardo , Espinoza-Andrade Sebastián , Franco-Sotomayor Greta , Benítez-Medina José Manuel , Jiménez-Pizarro Natalia , Cárdenas-Franco Camilo , Granda Juan Carlos , Jouvin Jose Luis , Orlando Solon Alberto , Hermoso de Mendoza Javier , García-Bereguiain Miguel Ángel
Published: February 2024

Objective

Tuberculosis (TB) is a major public health concern in Ecuador and Colombia, considering that both countries are high-burden TB settings. Molecular epidemiology is crucial to understand the transmission dynamics of Mycobacterium tuberculosis complex (MTBC) and to identify active transmission clusters of regional importance.

Methods

We studied the potential transmission of TB between Colombia and Ecuador through the analysis of the population structure of MTBC lineages circulating in the Ecuadorian province of Esmeraldas at the border with Colombia. A total of 105 MTBC strains were characterized by 24-loci MIRU-VNTR and spoligotyping.

Results

MTBC lineage 4 is only present in Esmeraldas; no MTBC strains belonging to Lineage 2–sublineage Beijing were found despite its presence in other provinces of Ecuador and, in Colombia. Genotyping results revealed a high degree of diversity for MTBC in Esmeraldas: Neither active transmission clusters within this province nor including MTBC strains from Colombia or other provinces of Ecuador were found.

Conclusion

Our data suggest that tuberculosis dynamics in this rural and isolated area may be not related to highly transmitted strains but could be influenced by other health determinants that favor TB relapse such as poverty and poor health system access. Further studies including a larger number of MTBC strains from Esmeraldas are necessary to test this hypothesis.
The presence of antibiotic-resistant bacteria at four Norwegian wastewater treatment plants: seasonal and wastewater-source effects
Authors: Basiry Daniel , Kommedal Roald , Kaster Krista Michelle
Published: February 2024

Wastewater treatment plants receive low concentrations of antibiotics. Residual concentrations of antibiotics in the effluent may accelerate the development of antibiotic resistance in the receiving environments. Monitoring of antimicrobial resistance genes (ARGs) in countries with strict regulation of antibiotic use is important in gaining knowledge of how effective these policies are in preventing the emergence of ARGs or whether other strategies are required, for example, at-source treatment of hospital effluents. This study evaluates the presence of certain common resistance genes (blaSHV-1, blaTEM-1, msrA, ermA, ermC, tetM, tetL, tetA, vanA, and vanC) in the influent, sludge, and effluent of four wastewater treatment plants (WWTPs) in the North Jæren region of Norway at two different sampling times (January and May). These WWTPs vary in drainage area and wastewater composition and were selected based on their differing wastewater characteristics. Randomly selected colonies from the activated sludge samples were used to determine the minimum inhibitory concentration (MIC) for ampicillin, vancomycin, and tetracycline. In addition, variations in the bacterial composition of the wastewater were characterized via 16S rRNA sequencing and were analyzed in terms of bacterial host taxa that explain the presence of the ARGs in wastewater. The MIC tests revealed MIC90 values of >128 µg/mL for ampicillin, ≥128 µg/mL for vancomycin, and 32 µg/mL for tetracycline. In addition, the three resistance genes, ermB, tetA, and tetM, that were present in the influent and activated sludge were still present in the effluent. These results indicate that WWTPs represent a direct route into the environment for resistance genes and do not significantly reduce their abundance. Hence, the development of treatment methods for the removal of these genes from WWTPs in the future is of utmost importance.
Comparison of daily step count between the Fitbit Inspire 3 and the activPAL 3 in adults with transtibial amputation
Authors: Leister Kyle R. , Burke Sara E. , Kim Joon Young , Duenas Victor H. , Barreira Tiago V.
Published: February 2024

Introduction

Physical activity has significant positive effects on health. Accelerometers can be used to track daily physical activity. The Fitbit Inspire 3 is a commercially available health and fitness tracker, but its validity for tracking steps among individuals with transtibial amputation has not been examined. Therefore, the purpose of this study was to evaluate the concurrent validity of the Fitbit Inspire 3 for assessing free-living daily steps in adults with transtibial amputation.

Methods

Participants (n = 79) completed a general health survey and were provided with a Fitbit Inspire 3 and activPAL 3 accelerometer to wear concurrently for seven days in their home environment. Relationships between the activPAL and Fitbit Inspire 3 were examined using Pearson's Correlation. Paired samples t-tests, mean difference, mean absolute difference, and equivalence testing were used to compared daily step counts between Fitbit Inspire 3 and activPAL 3.

Results

Average step counts were 5,768 ± 3,750 (mean ± SD) and 4,674 ± 3,081 by the Fitbit Inspire 3 and activPAL, respectively. A high correlation (r = 0.93) but significant mean difference was found between the activPAL 3 and Fitbit Inspire 3 (p < 0.001). The mean absolute difference between the devices was 1,347 ± 1,184 steps. On average, the Fitbit Inspire 3 counted 1,094 ± 1,423 more daily steps than the activPAL 3. Equivalency could not be claimed between the devices.

Discussion

The Fitbit Inspire 3 counted more steps compared to the activPAL. Because of the significant mean differences and the large mean absolute difference between the devices, the activPAL 3 and Fitbit Inspire 3 are not interchangeable for estimating physical activity in individuals with transtibial amputation. However, due to the high correlation, the devices will produce similar classification rankings based on step counts.
Transport-related social exclusion and mobility in developing countries: the South African case
Authors: Luke Rose
Published: February 2024

Introduction

Transport-related social exclusion has been studied in many countries, and from many different perspectives. In Africa, however, there is little recent research into the phenomenon, even though African cities tend to have poor transport services, urban sprawl is extensive usually resulting in long travel times and high travel costs, and certain areas experience high levels of crime. By implication, there are several factors that impact the ability of people to access economic and societal opportunities, however these are not well documented. Building on previous work from several authors, this research sought to describe transport-related social exclusion in a large metropolitan area in an emerging economy.

Methods

Using a qualitative methodology, 60 interviews were conducted with City of Johannesburg residents. The data was analyzed using manual thematic and classic content analysis.

Results

The study found that residents often did not have access to services due to availability, but also that economic, geographic and fear-based exclusion were particularly prevalent in the sample, although there were several other psychological exclusion dimensions, as well as information exclusion.

Discussion

This study is the first recent study in South Africa to consider a wide range of commuters from varying demographic strata, thus providing a novel perspective on TRSE in a major urban area in the country. The study recommends that policy actions be considered, taking into account the minibus taxi industry, which is often overlooked in government policy. TRSE should also be an inherent element in urban (and other) transport planning, as well as take into account the broader societal realities faced by citizens. Future research directions include conducting broader quantitative studies across a variety of urban areas in South Africa, and beyond, to determine specific TRSE dimensions in various urban areas. Policy analysis is also recommended, to determine TRSE policy gaps and align interventions with specific commuter needs.
The Mysterious World of Mosquitoes and Disease
Authors: Ciubotariu Ilinca I. , Markle Hannah L. , Gebhardt Mary E. , Norris Douglas E.
Published: February 2024

If someone were to ask you, “What is the deadliest animal in the world?” you might not think the answer would be an insect smaller than a thumb tack! However, the deadliest animal in the world is in fact the mosquito! While “mosquito” means “little fly” and may sound harmless, mosquitoes can actually transmit devastating diseases to humans, resulting in over 600,000 deaths worldwide every year. This is almost as high as the number of deaths from cancer, the second leading cause of death in the United States. Mosquitoes are found all over the world and, chances are, you have encountered them (and their itchy bites!) yourself. Keep reading to learn more about these creatures, including how they live, the many types of mosquitoes that exist, and the dangerous diseases they can transmit to humans.
Study and analysis of the correlation between lumbar spondylolisthesis and Modic changes
Authors: Li Guang-qing , Kang Xiao , Li Wei , Pei Shi-shen
Published: February 2024

Background

This study aimed to explore the risk factors of Modic changes in lumbar spondylolisthesis.

Methods

The distribution of Modic changes in different types of lumbar spondylolisthesis, degree of spondylolisthesis, and degree of intervertebral disc degeneration in patients with lumbar spondylolisthesis was observed and analyzed. Statistical analysis was conducted to assess whether intervertebral disc degeneration, local mechanical changes, etc. affect the occurrence of Modic changes. The risk factors of Modic changes in lumbar spondylolisthesis were further illustrated.

Results

The age in the lumbar spondylolisthesis with Modic changes group was younger than that in the lumbar spondylolisthesis without Modic changes group, and the bone mineral density was better in the lumbar spondylolisthesis with Modic changes group than that in the lumbar spondylolisthesis without Modic changes group, P < 0.05. The two groups statistically differed in intervertebral disc height (IDH) and disc angle on magnetic resonance imaging (MRI). In the classification of Modic changes, the incidence of type II was the highest. The incidence of Modic changes is higher in isthmic spondylolisthesis than in degenerative spondylolisthesis. With the aggravation of lumbar spondylolisthesis and intervertebral disc degeneration, the incidence of Modic changes gradually increased. Modic changes are most commonly seen in both the upper and lower endplates. Logistic regression analysis showed that the occurrence of Modic changes in lumbar spondylolisthesis was significantly correlated with IDH, disc angle on MRI, type of spondylolisthesis, degree of spondylolisthesis, and degree of intervertebral disc degeneration, P < 0.05.

Conclusions

The occurrence of Modic changes is related to the type of spondylolisthesis, the degree of spondylolisthesis, the degree of disc degeneration, the decrease of intervertebral disc height, and local stress angulation.
Bovine mortality: the utility of two data sources for the provision of population-level surveillance intelligence
Authors: Eze Jude I. , Correia-Gomes Carla , Gunn George J. , Tongue Sue C.
Published: February 2024

Introduction

The use of existing data to provide surveillance intelligence is widely advocated but often presents considerable challenges. Two data sources could be used as proxies for the mortality experienced by the Scottish cattle population: deaths recorded in the mandatory register [Cattle Tracing System (CTS)] and fallen stock collections by the National Fallen Stock Company (NSFCo) with a nationwide voluntary membership.

Methods

Data for the period 2011–2016 were described and compared to establish their strengths and limitations. Similarities and differences in their temporal, seasonal and spatial patterns were examined overall, at postcode area level and for different age groups. Temporal aberration detection algorithms (TADA) were fitted.

Results

Broadly, similar patterns were observed in the two datasets; however, there were some notable differences. The observed seasonal, annual and spatial patterns match expectations, given knowledge of Scottish cattle production systems. The registry data provide more comprehensive coverage of all areas of Scotland, while collections data provide a more comprehensive measure of the mortality experienced in 0–1-month-old calves.

Discussion

Consequently, estimates of early calf mortality and their impact on the livestock sector made using CTS, or successor registers, will be under-estimates. This may apply to other registry-based systems. Fitted TADA detected points of deviations from expected norms some of which coincided in the two datasets; one with a known external event that caused increased mortality. We have demonstrated that both data sources do have the potential to be utilized to provide measures of mortality in the Scottish cattle population that could inform surveillance activities. While neither is perfect, they are complementary. Each has strengths and weaknesses, so ideally, a system where they are analyzed and interpreted in parallel would optimize the information obtained for surveillance purposes for epidemiologists, risk managers, animal health policy-makers and the wider livestock industry sector. This study provides a foundation on which to build an operational system. Further development will require improvements in the timeliness of data availability and further investment of resources.
Digestive problems in rabbit production: moving in the wrong direction?
Authors: van der Sluis Malou , van Zeeland Yvonne R. A. , de Greef Karel H.
Published: February 2024

Digestive problems, both those with a clear pathogenic origin (e.g., Escherichia coli) and those without obvious pathogen involvement [e.g., syndromes like epizootic rabbit enteropathy (ERE)], are common in production rabbits and account for the majority of losses in meat rabbit production. A multitude of nutritional, genetic and housing factors have been found to play a role in the occurrence of digestive problems. However, the exact early pathophysiological mechanism, including the links between aforementioned risk factors and subsequent development and expression of gastrointestinal disease, is less clear, especially in non-specific enteropathies without obvious pathogen involvement. In this review, we aim to shed more light on the derailment of the normal gastrointestinal functioning in rabbits. We discuss a conceptual integrated view of this derailment, based on an “overload” pathway and a “chymus jam” pathway, which may occur simultaneously and interact. The “overload” pathway centers around exposure to excess amounts of easily fermentable substrate (e.g., starch and protein) that might be incompletely digested prior to entering the caecum. Once there, hyperfermentation may result in changes in caecal pH and inhibition of the normal microflora. The second pathway centers around a chymus jam resulting from a compromised passage rate. Here, reduced hindgut motility (e.g., resulting from stress or limited fiber supply) leads to reduced flow of digesta and increased caecal retention times, which might lead to the production of abnormal caecal fermentation products and subsequent inhibition of the normal microflora. A central role in the presumed mechanism is attributed to the fusus coli. We discuss the suggested mechanisms behind both pathways, as well as the empirical substantiation and alignment between theoretical concepts and observations in practice. The proposed hypotheses may explain the effect of time-based restriction to prevent ERE, which is widely applied in practice but to date not really understood, and suggest that the particle size of fiber may be a key point in the normal functioning of the colon and fusus coli. Further insight into the circumstances leading to the derailment of physiological processes in the rabbit hindgut could provide a meaningful starting point to help improve their gastrointestinal resilience.
Disposable Voltammetric Immunosensor for Determination and Quantification of Biomarker CA 15-3 in Biological Specimens
Authors: Oliveira Ana Elisa F. , Pereira Arnaldo César , Resende Mayra A. C. , Ferreira Lucas Franco
Published: February 2024

A disposable voltammetric immunosensor was developed to measure breast cancer biomarker 15-3 (CA 15-3) in human saliva and serum samples. Screen-printed paper-based electrodes (f-SPE) previously fabricated by our research group using homemade conductive inks were used as transducers, which were later modified only with gold nanoparticles to immobilize anti-CA 15-3 antibodies. The sensor was operated using antigen–antibody interactions in conjunction with a redox species (ferrocyanide potassium) for the indirect determination of the CA 15-3 antigen. The device characterization involved atomic force microscopy (AFM) and electrochemical analysis. Optimization of the construction and response of the immunosensor was achieved at incubation times of 6 h for anti-CA 15-3, 1 h for bovine serum albumin, and 1 h for interaction with CA 15-3. The sensor displays a linear range between 2 and 16 U/mL, with a sensitivity of 0.012 μA/U mL−1, a limit of detection (LOD) of 0.56 U/mL, and a limit of quantification (LOQ) of 1.88 U/mL. The interfering substances minimally affected the signal, with 4.94% response variation, and the reproducibility of the immunosensor demonstrated a relative standard deviation (RSD) of 5.65%. The sensor successfully determined the CA 15-3 concentration in human serum and saliva, demonstrating its potential for clinical analysis.
Experimental Study on Cyclic Shear Performance of the Four-Way Geogrid Reinforcement–Soil Interface
Authors: Zhang Mengxi , Ruan Xibo , Jiang Lei
Published: February 2024

This paper presents the results of horizontal cyclic direct shear tests at the reinforced soil interface of a four-way polypropylene geogrid reinforced sandy soil. The influence of normal stress and shear displacement amplitude on the shear stress, shear stiffness, and damping ratio of the reinforced soil interface are evaluated by varying the normal stress and shear displacement amplitude. Dynamic shear characteristics of reinforced soil interface under normal constant load were investigated by using a large dynamic straight shear apparatus. Peak interface strength increases with increasing amplitude of normal stress and shear displacement amplitude. The larger the normal stress and shear displacement amplitude, the fewer cycles are needed to attain peak interface strength. At low-magnitude normal stress levels, the peak shear stress and shear stiffness tend to stabilize after an initial increase during the cycling process, and the damping ratio decreases and then stabilizes with the increase in the number of cycles; whereas when the normal stress level is high, the peak shear stress and shear stiffness increase and then decrease during the cycling process and eventually stabilize, and the damping ratio decreases and then increases and finally stabilizes with the increase in the number of cycles. Moreover, under the same number of cycles, the corresponding shear stiffness decreases with an increase in shear displacement amplitude, while the damping ratio increases.
Methods for Testing Meniscal Repair Using a 3D-Printed Meniscus
Authors: Nelson Andrew , Voinier Steven , Tran Jeremy , Gilchrist Kristin H. , Helgeson Melvin , Ho Vincent B. , Klarmann George J.
Published: February 2024

Torn and damaged menisci resulting from trauma are very common knee injuries, which can cause pain and mobility limitations and lead to osteoarthritis. Meniscal injuries can require surgery to repair the tissue damage and restore mobility. Here we describe the biomechanical testing of a 3D-printed meniscus to illustrate methods to determine if it has the strength and durability to effectively repair meniscal tears and restore knee biomechanics. This work was designed to demonstrate the steps needed to test novel meniscus repair devices prior to moving toward animal testing. The first testing step determined the ability of the 3D-printed meniscus to withstand surgical fixation by measuring the suture pull-out force. We show that vertical 2/0 silk or Fiberwire sutures need an average of 1.4 or 1.8 N, respectively, to pull through the meniscus, while horizontal sutures need only 0.7 and 1.2 N, respectively. The next step measured the compressive strength of normal, damaged, and repaired porcine meniscus tissue. Here, we show that meniscectomy decreased the stiffness of meniscus tissue from 26.7 ± 0.85 N to 7.43 ± 0.81 N at 25% strain. Menisci repaired with the 3D-printed tissue restored 66% of the measured force at 25% strain. The final step measured the contact pressures and areas in an ex vivo porcine knee before and after meniscal repair was made with the 3D-printed meniscus tissue. The example 3D-printed meniscus was successfully sutured into the porcine knee joint but failed to restore normal knee contact pressures. This work demonstrates the need for an iterative biomechanical testing process of biomaterial development, 3D-printing optimization, and knee kinematics to develop a durable and functional meniscus repair device. In summary, the methods described here serve as a guide for the functional evaluation of novel meniscus repair devices.