bluuebunny/crossref_metadata_2025 huggingface dataset:
An easy and practical guide for imaging infection/inflammation by [18F]FDG PET/CT
Authors: Signore Alberto , Casali Massimiliano , Lauri Chiara
Published: June 2021

Aim

The aim of this mini-review was to summarize the role of positron emission tomography/computed tomography (PET/CT) with 18Fluorine-fluorodeoxyglucose ([18F]FDG) in inflammatory and infective processes, based on the published scientific evidence.

Methods

We analysed clinical indications, patient preparation, image acquisition protocols, image interpretation, pitfalls and how to make the report of cardio-vascular diseases, musculoskeletal diseases and other inflammatory and infective systemic diseases.

Results of this analysis are shown in practical tables, easy to understand for daily routine consultation.

Conclusions

Despite [18F]FDG is currently used in several inflammatory and infective diseases, standardized interpretation criteria are still needed in most cases. It is, therefore, foreseen the execution of multicentre clinical studies that, by adopting the same acquisition and interpretation criteria, may contribute to the standardization of this imaging modality.
Third reported case of rare necrolytic migratory erythema associated with bacteraemia due to severe zinc deficiency after revisional Roux-En-Y gastric bypass: case report and literature review
Authors: Salaheldin Yassmin , El Ansari Walid , Aljaloudi Esraa , Elhag Wahiba
Published: June 2021

Introduction

Obesity is a risk factor for zinc deficiency. After bariatric surgery, non-compliance to diet/vitamin supplements, surgical complications leading to vomiting/diarrhea, poor follow-up and malabsorption can precipitate or exacerbate pre-existing zinc deficiency.

Case report

We report a patient with rare necrolytic migratory erythema associated with bacteraemia due to severe zinc deficiency after revisional Roux-en-Y gastric bypass (following primary laparoscopic sleeve gastrectomy).

Conclusion

Bariatric teams should screen patients before bariatric surgery for nutritional deficiencies and continue surveillance of their nutritional status after surgery. They should maintain a high index of suspicion for zinc deficiency in patients with skin rash after bariatric surgery.

Level of evidence

Level V, case report.
Online social networks security and privacy: comprehensive review and analysis
Authors: Jain Ankit Kumar , Sahoo Somya Ranjan , Kaubiyal Jyoti
Published: June 2021

With fast-growing technology, online social networks (OSNs) have exploded in popularity over the past few years. The pivotal reason behind this phenomenon happens to be the ability of OSNs to provide a platform for users to connect with their family, friends, and colleagues. The information shared in social network and media spreads very fast, almost instantaneously which makes it attractive for attackers to gain information. Secrecy and surety of OSNs need to be inquired from various positions. There are numerous security and privacy issues related to the user’s shared information especially when a user uploads personal content such as photos, videos, and audios. The attacker can maliciously use shared information for illegitimate purposes. The risks are even higher if children are targeted. To address these issues, this paper presents a thorough review of different security and privacy threats and existing solutions that can provide security to social network users. We have also discussed OSN attacks on various OSN web applications by citing some statistics reports. In addition to this, we have discussed numerous defensive approaches to OSN security. Finally, this survey discusses open issues, challenges, and relevant security guidelines to achieve trustworthiness in online social networks.
Chained graphs and some applications
Authors: Concas Anna , Reichel Lothar , Rodriguez Giuseppe , Zhang Yunzi
Published: June 2021

This paper introduces the notions of chained and semi-chained graphs. The chain of a graph, when existent, refines the notion of bipartivity and conveys important structural information. Also the notion of a center vertex $$v_c$$

v
c

is introduced. It is a vertex, whose sum of p powers of distances to all other vertices in the graph is minimal, where the distance between a pair of vertices $$\{v_c,v\}$$

{

v
c

,
v
}

is measured by the minimal number of edges that have to be traversed to go from $$v_c$$

v
c

to v. This concept extends the definition of closeness centrality. Applications in which the center node is important include information transmission and city planning. Algorithms for the identification of approximate central nodes are provided and computed examples are presented.
Discourses in Subjective Experience on Rehabilitation
Authors: Ylimaula Sirpa , Suorsa Teemu
Published: June 2021

In Finland, youth workshops support participants’ everyday management, social empowerment and employability skills, facilitating their access to education and work. In this study, we analysed conversations with ten participants about their experiences in non-formal learning workshops in Northern Finland. We explore how participants described personal transformation borne by workshop participation and how the prevalent discourses on youth (un)employment manifest in participants’ reasons for action. In the analysis, we identified (1) how participants assessed their situations; (2) their thoughts, feelings and actions in relation to their situations; and (3) their grounds for these thoughts, feelings and actions. The societal dimension of subjective experience is articulated by identifying the prevalent societal discourses featured in participants’ descriptions. The analysis showed that participants described themselves as vulnerable prior to the intervention and motivated after the intervention. Participants experienced tailored support as being significant in fostering the change. Individual descriptions of how the learning and training conditions were experienced constitute valuable knowledge about the possible ways of acting and experiencing in relation to common structures. With the help of these descriptions, it would be valuable for researchers and participants to continue the conversation about the discourses to enhance the participants’ conscious participation in maintaining and changing their living conditions.
The ‘3CE2CE’ Framework—Change Management Towards a Circular Economy: Opportunities for Agribusiness
Authors: Yang Nan-Hua Nadja , Bertassini Ana Carolina , Mendes Jéssica Alves Justo , Gerolamo Mateus Cecílio
Published: June 2021

For the transition towards a circular economy (CE), organisations have to be prepared to adapt to major changes. Thus, the concept and implementation of change management (CM) will be essential to an organisation’s success during this transformative period. Studies have shown that organisational CE barriers were more significant than individual CE barriers. To overcome such obstacles, the most appropriate set of managerial practices should be carefully considered. These barriers also have the potential to influence the agricultural sector, which seeks to adopt more sustainable ways of production. The goal of this paper is to propose a solution framework based on CM strategies to overcome organisational challenges posed by a CE, especially for agribusinesses. To accomplish this objectively, a systematic literature review and a content analysis were conducted. The common errors in CM within the implementation process and the main CE barriers were identified and classified. An in-depth analysis of the issue’s roots led to a solid understanding of how to tackle such CM problems. This paper presents an overview of organisational CE barriers verified in the agricultural context, the common errors in CM, and the correlation between these findings. The two areas were then combined in a matrix that shows the connection between common errors in CM errors and CE barriers. Based on this result, a solution framework called 3CE2CE was developed that provides a step-by-step guide on how organisations can successfully undergo transformation processes towards a CE with the principles of CM.
Acceptability of Health Professionals’ Address of Sexuality and Erectile Dysfunction - A Qualitative Interview Study with Men in Cardiac Rehabilitation
Authors: Gerbild Helle , Areskoug-Josefsson Kristina , Larsen Camilla Marie , Laursen Birgitte Schantz
Published: June 2021

Introduction

In the developing phase of the complex health intervention: Physical Activity to reduce Vascular Erectile Dysfunction (PAVED), it is crucial to explore whether men can accept the communicative component regarding information that regular aerobe Physical Activity can reduce Erectile Dysfunction (i-PAVED). This information is provided by health professionals (HPs) in cardiac rehabilitation, where sexuality issues such as erectile dysfunction (ED) are otherwise rarely addressed.

Aim

To explore how acceptance of cardiac HPs’ address of sexuality, ED, and i-PAVED can be identified in men's narratives.

Methods

In this descriptive qualitative study, we conducted semi-structured individual interviews with 20 men (range 48-78 years of age) attending municipal cardiac secondary prevention and rehabilitation programmes on their acceptance of HPs’ address of sexuality, ED, and i-PAVED. The Theoretical Framework of Acceptability components (affective attitude, burden, ethicality, intervention coherence, perceived effectiveness, opportunity costs and self-efficacy) and three temporal perspectives (retrospective, concurrent and prospective) were used in the concept-driven first step of a content analysis, which was followed by a thematically data-driven second step.

Main Outcome Measures

Men anticipated and experiential acceptance was identified in six out of seven components of Theoretical Framework of Acceptability.

Results

Men acceptance was identified as “expression of interest,” “addressing sexuality,” “attitudes and values,” “understandable and meaningful,” “insights” and “motivation,” whereas no narratives were identified in relation to the component of opportunity costs.

Conclusion

As an aspect of the development of the complex cardiovascular health care intervention PAVED, this qualitative study showed that men attending cardiac secondary prevention and rehabilitation seemed to prospectively accept the communicative component of PAVED being HPs’ address of sexuality, ED, and i-PAVED, if the HPs are professional, educated and competent in the field of sexual health.
Logic, geometry and visualisation of the body in Acquapendente’s rediscovered Methodus anatomica (1579)
Authors: Bigotti Fabrizio
Published: June 2021

The article provides the first description and analysis of the recently rediscovered manuscript titledMethodus anatomicaby Girolamo Fabrici da Acquapendente (1533–1619). Acquapendente was one of the most important anatomists in late sixteenth-century Europe and played an instrumental role as Harvey’s teacher in Padua towards the latter’s discovery of the circulation of the blood. The manuscript provides first-hand testimony as to how anatomy was administered in Padua in the post-Vesalian era and sheds light on a number of otherwise unknown aspects of the development of the anatomical method. Chiefly among these is the attention devoted by Acquapendente tohistoria, as a way to order sensory data in a consistent way, which draws widely from the geometrical method and from the contemporary debate on the discretisation of continuous quantities.
Warfare and the launch of medical reform in Britain, 1793–1811
Authors: Mackintosh Alan
Published: June 2021

Until the beginning of the nineteenth century, registering and regulating the training of any medical practitioners in Britain had rarely been attempted, unlike in many other European countries. During the Revolutionary War with France, fevers swept through British armies, leading to numerous fatalities and crushing military defeats, especially in the disastrous expedition to St Domingo. The problem, as forcibly advocated by Robert Jackson, the leading expert on military fevers, seemed to be poor medical care due to both lack of compulsory medical training and the unsuitability of whatever training was available for army medical practitioners. With the simultaneous rapid advance of French military and civilian medical training and the threat of a French invasion, regulating British medical training and excluding the unqualified became a military necessity, and suddenly medical reform was receiving widespread attention. Emphasising the benefits to the Britain’s fighting ability, the reform effort, led by Edward Harrison, a very provincial Lincolnshire physician, under the patronage of Sir Joseph Banks, the President of the Royal Society, gained the support of leading politicians, including three Prime Ministers. For a short time, comprehensive medical reform seemed inevitable: but the opposition of the medical corporations, especially the London College of Physicians, could not be circumvented, and although Harrison persisted in his efforts for 6 years, no legislation was achieved. Nevertheless, within months, the Association of Apothecaries continued the process by pressing for a more limited reform, culminating in the 1815 Apothecaries Act. The long march towards the full regulation of doctors in Britain was started by the perceived military needs of the country during the war with France.
Joy and Love in Zora Neale Hurston and Dorothy Waring’s 1944 Black Feminist MusicalPolk County
Authors: Glover Eric M.
Published: June 2021

What makes Zora Neale Hurston different as a musical theatre writer is her concern about the creation of safe spaces for black women actors. By looking at the theatrical representation of black women in Hurston and Dorothy Waring’sPolk County, it is possible to see ways in which they resist intersecting oppressions of gender and race. Hurston’s adaptation of the blues and folk music for the musical is also subject to analysis, as is her lasting impact on musical theatre.
Foreign Assembly Toshiki Okada’s Time’s Journey through a Room in the US
Authors: Martin Carol
Published: June 2021

Okada is one of the most internationally produced contemporary Japanese playwrights. American directors approach his work both as uniquely Japanese and as a synecdoche for the world. The story of Okada’s web of institutional, professional, and personal relationships is an object lesson in the foreign assembly of international works.
Expectations, values, preferences and experiences of Hungarian primary care population when accessing services: Evaluation of the patient’s questionnaires of the international QUALICOPC study
Authors: Nánási Anna , Ungvári Tímea , Kolozsvári László R. , Harsányi Szilvia , Jancsó Zoltán , Lánczi Levente I. , Mester Lajos , Móczár Csaba , Semanova Csilla , Schmidt Péter , Szidor Judit , Torzsa Péter , Végh Mária , Rurik Imre
Published: June 2021

Background:

Preferences and wishes of patients is an important indicator of primary health care provision, although there are differences between national primary care systems.

Aim:

The aim of this paper is to describe and evaluate the preferences and values of Hungarian primary care (PC) patients before accessing and to analyse their experiences after attending PC services.

Methods:

In the Hungarian arm of the European QUALICOPC Study, in 2013–2014, information was collected with questionnaires; the Patient Values contained 19 and the Patient Experiences had 41 multiple-choice questions.

Findings:

The questionnaires were filled by 2149 (840 men, 1309 women) using PC services, aged 49.1 (SD ± 16.7) years, 73% of them having chronic morbidities. Women preferred to be accompanied and rated their own health better. Patients in the lowest educational category and women visited their GPs more often, and they are consulted more frequently by other doctors as well. Men, older and secondary educated people reported more frequently chronic morbidities. Longer opening hours were preferred by patients with higher education. The most preferred expectations were availability and polite communication of doctors, not pressures on consultation time, clear instructions provided during consultations, shared decisions about treatments and options for consultations, the knowledge of the doctors concerning the living conditions, social and cultural backgrounds of patients, updated medical records, short waiting times, options for home visits, wide scope of professional competences and trust in the doctor.

Conclusion:

Wishes, preferences of patients and fulfilment were similar than described in other participating countries of the study. Although there are room to improve PC services, most of the questioned population were satisfied with the provision.
Cosmic connections: James Croll's influence on his contemporaries and his successors
Authors: FLEMING James R.
Published: June 2021

This paper examines the astronomical theory of ice ages of James Croll (1821–1890), its influence on contemporaries John Tyndall, Charles Lyell, and Charles Darwin, and the subsequent development of climate change science, giving special attention to the work of Svante Arrhenius, Nils Ekholm, and G. S. Callendar (for the carbon dioxide theory), and Milutin Milanković (for the astronomical theory). Croll's insight that the orbital elements triggered feedbacks leading to complex changes – in seasonality, ocean currents, ice sheets, radiative forcing, plant and animal life, and climate in general – placed his theory of the Glacial Epoch at the nexus of astronomy, terrestrial physics, and geology. He referred to climate change as the most important problem in terrestrial physics, and the one which will ultimately prove the most far reaching in its consequences. He was an autodidact deeply involved in philosophy and an early proponent of what came to be called ‘cosmic physics’ – later known as ‘Earth-system science.’ Croll opened up new dimensions of the ‘climate controversy’ that continue today in the interplay of geological and human influences on climate.
Lyell, the Geikies and Croll's observations on terrestrial glacial sediments and landforms
Authors: ROSE James
Published: June 2021

Within the context of the work and achievements of James Croll, this paper reviews the records of direct observations of glacial landforms and sediments made by Charles Lyell, Archibald and James Geikie and James Croll himself, in order to evaluate their contributions to the sciences of glacial geology and Quaternary environmental change. The paper outlines the social and physical environment of Croll's youth and contrasts this with the status and experiences of Lyell and the Geikies. It also outlines the character and role of the ‘Glasgow School’ of geologists, who stimulated Croll's interest into the causes of climate change and directed his focus to the glacial and ‘interglacial’ deposits of central Scotland. Contributions are outlined in chronological order, drawing attention to: (i) Lyell's high-quality observations and interpretations of glacial features in Glen Clova and Strathmore and his subsequent rejection of the glacial theory in favour of processes attributed to floating icebergs; (ii) the significant impact of Archibald Geikie's 1863 paper on the ‘glacial drift of Scotland’, which firmly established the land-ice theory; (iii) the fact that, despite James Croll's inherent dislike of geology and fieldwork, he provided high-quality descriptions and interpretations of the landforms and sediments of central Scotland in order to test his theory of climate change; and (iv) the great communication skills of James Geikie, enhanced by contacts and evidence from around the world. It is concluded that whilst direct observations of glacial landforms and sediments were critical to the long-term development of the study of glaciation, the acceptance of this theory was dependent also upon the skills, personality and status of the Geikies and Croll, who developed and promoted the concepts. Sadly, the subsequent rejection of the land-ice concept by Lyell resulted in the same factors challenging the acceptance of the glacial theory.
Science, metaphysics and Calvinism: the God of James Croll
Authors: FINNEGAN Diarmid A.
Published: June 2021

Science, for James Croll, began and ended in metaphysics. Metaphysics, in turn, provided proof of a First and Final Cause of all things. This proof rested on two metaphysical principles: that every event must have a cause, and that the determination of a cause is distinct from its production. This argument emerged from his deeply held religious commitments. As a 17-year-old, he converted to a Calvinist and evangelical form of Christianity. After a period of questioning the Calvinist system, he embraced it again through reading the famous treatise on the will by the New England theologian, Jonathan Edwards. This determinedly metaphysical work, which engaged as much with Enlightenment thought as with Calvinism, defended the view that the will was not a self-determining cause of human action. This ‘hard case’ provided the basis for a larger claim that every act whatever has a cause, and that the production of an act was different from its determination. In part through reading Edwards, Croll remained a devout and convinced ‘moderate’ Calvinist for the rest of his life. He also developed a deep love of metaphysics and became convinced that without it, everything, including science, remained confused and in darkness. For Croll, even the most basic science could not be properly conducted without prior metaphysical principles. But this was more than just an argument about the philosophical foundations of scientific inquiry. It was also based on Croll's conviction that the cosmos, earth history and life (including his own) was fully determined by a supreme and perfect intellect. This conviction entered into the marrow of Croll's scientific theories and shaped his interpretation of the twists and turns of his own life. In short, to take seriously Croll's own self-understanding, we need to allow him to ‘do God’.
Gender quotas and public demand for increasing women’s representation in politics: an analysis of 28 European countries
Authors: Fernández Juan J. , Valiente Celia
Published: June 2021

Female representation in political decision-making positions is now a salient issue in public discussions throughout Europe. Understanding public attitudes towards a more balanced gender distribution in politics remains limited, however. Using a 2017 Eurobarometer, we focus on cross-national differences in public support for increased female participation in politics to address this limitation. Building on the policy feedbacks literature, we stress the role of gender quotas. We argue that quotas – as legislative devices usually adopted through elite-driven initiatives – stimulate support for stronger female representation. Ensuing debates on quotas raise individual awareness about the underrepresentation of women – informational effect – and, once adopted, give a clear signal that persistent gender imbalance is a social problem to be redressed – normative effect. Our empirical analysis supports this argument. Citizens in countries with gender quotas display stronger support for increased female participation in politics.
Perceived Autonomy Moderates the Relation of Proactive Personality With Job Crafting
Authors: Albert Melissa A. , Highhouse Scott
Published: June 2021

Abstract. A two-wave study with a sample of employed adults ( n = 219) examined the interaction of proactive personality and perceived autonomy in the prediction of self-reported job crafting. We found that proactive personality was associated with increased job crafting, and this relation was stronger among people who reported experiencing a higher level of job autonomy.
CRISPR/Cas9-mediated targeted mutation reveals a role for AN4 rather than DPL in regulating venation formation in the corolla tube of Petunia hybrida
Authors: Zhang Bin , Xu Xiaojing , Huang Renwei , Yang Sha , Li Mingyang , Guo Yulong
Published: June 2021

Venation is a common anthocyanin pattern displayed in flowers that confers important ornamental traits to plants. An anthocyanin-related R2R3-MYB transcription factor, DPL, has been proposed to regulate corolla tube venation in petunia plants. Here, however, we provide evidence redefining the role of DPL in petunia. A CRISPR/Cas9-mediated mutation of DPL resulted in the absence of the vein-associated anthocyanin pattern above the abaxial surface of the flower bud, but not corolla tube venation, thus indicating that DPL did not regulate the formation of corolla tube venation. Alternately, quantitative real-time PCR analysis demonstrated that the spatiotemporal expression pattern of another R2R3-MYB gene, AN4, coincided with the formation of corolla tube venation in petunia. Furthermore, overexpression of AN4 promoted anthocyanin accumulation by increasing the expression of anthocyanin biosynthesis genes. CRISPR/Cas9-mediated mutation of AN4 led to an absence of corolla tube venation, suggesting that this gene in fact determines this key plant trait. Taken together, the results presented here redefine the prime regulator of corolla tube venation, paving the way for further studies on the molecular mechanisms underlying the various venation patterns in petunia.
The diverse roles of cytokinins in regulating leaf development
Authors: Wu Wenqi , Du Kang , Kang Xiangyang , Wei Hairong
Published: June 2021

Leaves provide energy for plants, and consequently for animals, through photosynthesis. Despite their important functions, plant leaf developmental processes and their underlying mechanisms have not been well characterized. Here, we provide a holistic description of leaf developmental processes that is centered on cytokinins and their signaling functions. Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the initial stage of leaf formation; cytokinins and auxins, as well as their interaction, determine the phyllotaxis pattern. The activities of cytokinins in various regions of the leaf, especially at the margins, collectively determine the final leaf morphology (e.g., simple or compound). The area of a leaf is generally determined by the number and size of the cells in the leaf. Cytokinins promote cell division and increase cell expansion during the proliferation and expansion stages of leaf cell development, respectively. During leaf senescence, cytokinins reduce sugar accumulation, increase chlorophyll synthesis, and prolong the leaf photosynthetic period. We also briefly describe the roles of other hormones, including auxin and ethylene, during the whole leaf developmental process. In this study, we review the regulatory roles of cytokinins in various leaf developmental stages, with a focus on cytokinin metabolism and signal transduction processes, in order to shed light on the molecular mechanisms underlying leaf development.
Tissue and regional expression patterns of dicistronic tRNA–mRNA transcripts in grapevine (Vitis vinifera) and their evolutionary co-appearance with vasculature in land plants
Authors: Fabres Pastor Jullian , Anand Lakshay , Sai Na , Pederson Stephen , Zheng Fei , Stewart Alexander A. , Clements Benjamin , Lampugnani Edwin R. , Breen James , Gilliham Matthew , Tricker Penny , Rodríguez López Carlos M. , David Rakesh
Published: June 2021

Transfer RNAs (tRNA) are crucial adaptor molecules between messenger RNA (mRNA) and amino acids. Recent evidence in plants suggests that dicistronic tRNA-like structures also act as mobile signals for mRNA transcripts to move between distant tissues. Co-transcription is not a common feature in the plant nuclear genome and, in the few cases where polycistronic transcripts have been found, they include non-coding RNA species, such as small nucleolar RNAs and microRNAs. It is not known, however, the extent to which dicistronic transcripts of tRNA and mRNAs are expressed in field-grown plants, or the factors contributing to their expression. We analysed tRNA–mRNA dicistronic transcripts in the major horticultural crop grapevine (Vitis vinifera) using a novel pipeline developed to identify dicistronic transcripts from high-throughput RNA-sequencing data. We identified dicistronic tRNA–mRNA in leaf and berry samples from 22 commercial vineyards. Of the 124 tRNA genes that were expressed in both tissues, 18 tRNA were expressed forming part of 19 dicistronic tRNA–mRNAs. The presence and abundance of dicistronic molecules was tissue and geographic sub-region specific. In leaves, the expression patterns of dicistronic tRNA–mRNAs significantly correlated with tRNA expression, suggesting that their transcriptional regulation might be linked. We also found evidence of syntenic genomic arrangements of tRNAs and protein-coding genes between grapevine andArabidopsis thaliana, and widespread prevalence of dicistronic tRNA–mRNA transcripts among vascular land plants but no evidence of these transcripts in non-vascular lineages. This suggests that the appearance of plant vasculature and tRNA–mRNA occurred concurrently during the evolution of land plants.
Identification and characterization of GLOBE, a major gene controlling fruit shape and impacting fruit size and marketability in tomato
Authors: Sierra-Orozco Edgar , Shekasteband Reza , Illa-Berenguer Eudald , Snouffer Ashley , van der Knaap Esther , Lee Tong Geon , Hutton Samuel F.
Published: June 2021

Within large-fruited germplasm, fruit size is influenced by flat and globe shapes. Whereas flat fruits are smaller and retain better marketability, globe fruits are larger and more prone to cuticle disorders. Commercial hybrids are often developed from crosses between flat and globe shaped parents because flat shape is thought to be dominant and fruit size intermediate. The objectives of this study were to determine the genetic basis of flat/globe fruit shape in large-fruited fresh-market tomato germplasm and to characterize its effects on several fruit traits. Twenty-three advanced single plant selections from the Fla. 8000 × Fla. 8111B cross were selectively genotyped using a genome-wide SNP array, and inclusive composite interval mapping identified a single locus on the upper arm of chromosome 12 associated with shape, which we termed globe. A 238-plant F2 population and 69 recombinant inbred lines for this region from the same parents delimited globe to approximately 392-kilobases. A germplasm survey representing materials from multiple breeding programs demonstrated that the locus explains the flat/globe shape broadly. A single base insertion in an exon of Solyc12g006860, a gene annotated as a brassinosteroid hydroxylase, segregated completely with shape in all populations tested. CRISPR/Cas9 knock out plants confirmed this gene as underlying the globe locus. In silico analysis of the mutant allele of GLOBE among 595 wild and domesticated accessions suggested that the allele arose very late in the domestication process. Fruit measurements in three genetic backgrounds evidenced that globe impacts fruit size and several fruit shape attributes, pedicel length/width, and susceptibility of fruit to weather check. The mutant allele of GLOBE appears mostly recessive for all traits except fruit size where it acts additively.
A unified route for flavivirus structures uncovers essential pocket factors conserved across pathogenic viruses
Authors: Hardy Joshua M. , Newton Natalee D. , Modhiran Naphak , Scott Connor A. P. , Venugopal Hariprasad , Vet Laura J. , Young Paul R. , Hall Roy A. , Hobson-Peters Jody , Coulibaly Fasséli , Watterson Daniel
Published: June 2021

The epidemic emergence of relatively rare and geographically isolated flaviviruses adds to the ongoing disease burden of viruses such as dengue. Structural analysis is key to understand and combat these pathogens. Here, we present a chimeric platform based on an insect-specific flavivirus for the safe and rapid structural analysis of pathogenic viruses. We use this approach to resolve the architecture of two neurotropic viruses and a structure of dengue virus at 2.5  Å, the highest resolution for an enveloped virion. These reconstructions allow improved modelling of the stem region of the envelope protein, revealing two lipid-like ligands within highly conserved pockets. We show that these sites are essential for viral growth and important for viral maturation. These findings define a hallmark of flavivirus virions and a potential target for broad-spectrum antivirals and vaccine design. We anticipate the chimeric platform to be widely applicable for investigating flavivirus biology.
Quantum surface-response of metals revealed by acoustic graphene plasmons
Authors: Gonçalves P. A. D. , Christensen Thomas , Peres Nuno M. R. , Jauho Antti-Pekka , Epstein Itai , Koppens Frank H. L. , Soljačić Marin , Mortensen N. Asger
Published: June 2021

A quantitative understanding of the electromagnetic response of materials is essential for the precise engineering of maximal, versatile, and controllable light–matter interactions. Material surfaces, in particular, are prominent platforms for enhancing electromagnetic interactions and for tailoring chemical processes. However, at the deep nanoscale, the electromagnetic response of electron systems is significantly impacted by quantum surface-response at material interfaces, which is challenging to probe using standard optical techniques. Here, we show how ultraconfined acoustic graphene plasmons in graphene–dielectric–metal structures can be used to probe the quantum surface-response functions of nearby metals, here encoded through the so-called Feibelman d-parameters. Based on our theoretical formalism, we introduce a concrete proposal for experimentally inferring the low-frequency quantum response of metals from quantum shifts of the acoustic graphene plasmons dispersion, and demonstrate that the high field confinement of acoustic graphene plasmons can resolve intrinsically quantum mechanical electronic length-scales with subnanometer resolution. Our findings reveal a promising scheme to probe the quantum response of metals, and further suggest the utilization of acoustic graphene plasmons as plasmon rulers with ångström-scale accuracy.
Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis
Authors: Horisawa-Takada Yuki , Kodera Chisato , Takemoto Kazumasa , Sakashita Akihiko , Horisawa Kenichi , Maeda Ryo , Shimada Ryuki , Usuki Shingo , Fujimura Sayoko , Tani Naoki , Matsuura Kumi , Akiyama Tomohiko , Suzuki Atsushi , Niwa Hitoshi , Tachibana Makoto , Ohba Takashi , Katabuchi Hidetaka , Namekawa Satoshi H. , Araki Kimi , Ishiguro Kei-Ichiro
Published: June 2021

During spermatogenesis, meiosis is accompanied by a robust alteration in gene expression and chromatin status. However, it remains elusive how the meiotic transcriptional program is established to ensure completion of meiotic prophase. Here, we identify a protein complex that consists of germ-cell-specific zinc-finger protein ZFP541 and its interactor KCTD19 as the key transcriptional regulators in mouse meiotic prophase progression. Our genetic study shows that ZFP541 and KCTD19 are co-expressed from pachytene onward and play an essential role in the completion of the meiotic prophase program in the testis. Furthermore, our ChIP-seq and transcriptome analyses identify that ZFP541 binds to and suppresses a broad range of genes whose function is associated with biological processes of transcriptional regulation and covalent chromatin modification. The present study demonstrates that a germ-cell specific complex that contains ZFP541 and KCTD19 promotes the progression of meiotic prophase towards completion in male mice, and triggers the reconstruction of the transcriptional network and chromatin organization leading to post-meiotic development.
The impact of lymphedema on health-related quality of life up to 10 years after breast cancer treatment
Authors: Jørgensen Mads G. , Toyserkani Navid M. , Hansen Frederik G. , Bygum Anette , Sørensen Jens A.
Published: June 2021

The impact of breast cancer-related lymphedema (BCRL) on long-term quality of life is unknown. The aim of this study was to investigate the impact of BCRL on health-related quality of life (HRQoL) up to 10 years after breast cancer treatment. This regional population-based study enrolled patients treated for breast cancer with axillary lymph node dissection between January 1st 2007 and December 31th 2017. Follow up and assessments of the included patients were conducted between January 2019 and May 2020. The study outcome was HRQoL, evaluated with the Lymphedema Functioning, Disability and Health Questionnaire, the Disabilities of the Arm, Shoulder and Hand Questionnaire and the Short Form (36) Health Survey Questionnaire. Multivariate linear logistic regression models adjusted for confounders provided mean score differences (MDs) with 95% confidence intervals in each HRQoL scale and item. This study enrolled 244 patients with BCRL and 823 patients without BCRL. Patients with BCRL had significantly poorer HRQoL than patients without BCRL in 16 out of 18 HRQoL subscales, for example, in physical function (MDs 27, 95%CI: 24; 30), mental health (MDs 24, 95%CI: 21; 27) and social role functioning (MDs 20, 95%CI: 17; 23). Age, BMI, BCRL severity, hand and dominant arm affection had only minor impact on HRQoL (MDs < 5), suggesting a high degree of inter-individual variation in coping with lymphedema. This study showed that BCRL is associated with long-term impairments in HRQoL, especially affecting the physical and psychosocial domains. Surprisingly, BCRL diagnosis rather than clinical severity drove the largest impairments in HRQoL.
When primary care providers and smokers meet: a systematic review and metasynthesis
Authors: Manolios Emilie , Sibeoni Jordan , Teixeira Maria , Révah-Levy Anne , Verneuil Laurence , Jovic Ljiljana
Published: June 2021

Primary Care Providers (PCPs) often deal with patients on daily clinical practice without knowing anything about their smoking status and willingness to quit. The aim of this metasynthesis is to explore the PCPs and patients who are smokers perspectives regarding the issue of smoking cessation within primary care settings. It relies on the model of meta-ethnography and follows thematic synthesis procedures. Twenty-two studies are included, reporting on the view of 580 participants. Three main themes emerge: (i) What lacks, (ii) Some expectations but no request, and (iii) How to address the issue and induce patients’ motivation. Our results reveal a global feeling of a lack of legitimacy among PCPs when it comes to addressing the issue of tobacco and smoking cessation with their patients, even though they have developed creative strategies based on what is at the core of their practice, that is proximity, continuity, long-term and trustworthy relationship.
Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine
Authors: Zhang Jian , Fang Xin , Mao Yu , Qi Haochen , Wu Jayne , Liu Xiaoru , You Fangshuo , Zhao Wenci , Chen Ying , Zheng Lei
Published: June 2021

Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.
Advances in 3D peptide hydrogel models in cancer research
Authors: Xu Jingwen , Qi Guangyan , Wang Weiqun , Sun Xiuzhi Susan
Published: June 2021

In vitro cell culture models on monolayer surfaces (2D) have been widely adapted for identification of chemopreventive food compounds and food safety evaluation. However, the low correlation between 2D models and in vivo animal models has always been a concern; this gap is mainly caused by the lack of a three-dimensional (3D) extracellular microenvironment. In 2D models, cell behaviors and functionalities are altered, resulting in varied responses to external conditions (i.e., antioxidants) and hence leading to low predictability. Peptide hydrogel 3D scaffolding technologies, such as PGmatrix for cell culture, have been recently reported to grow organoid-like spheroids physiologically mimicking the 3D microenvironment that can be used as an in vitro 3D model for investigating cell activities, which is anticipated to improve the prediction rate. Thus, this review focuses on advances in 3D peptide hydrogels aiming to introduce 3D cell culture tools as in vitro 3D models for cancer-related research regarding food safety and nutraceuticals.
Identification of CRF89_BF, a new member of an HIV-1 circulating BF intersubtype recombinant form family widely spread in South America
Authors: Delgado Elena , Fernández-García Aurora , Pérez-Losada Marcos , Moreno-Lorenzo María , Fernández-Miranda Ismael , Benito Sonia , Montero Vanessa , Gil Horacio , Hernáez Silvia , Muñoz Josefa , Zubero-Sulibarria Miren Z. , García-Bodas Elena , Sánchez Mónica , del Romero Jorge , Rodríguez Carmen , Elorduy Luis , Bereciartua Elena , Culebras Esther , Rodríguez-Avial Icíar , Giménez-Alarcón María Luisa , Martín-Salas Carmen , Gómez-González Carmen , García-Irure José J. , Cenzual Gema , Martínez-Sapiña Ana , Maiques-Camarero María , Pérez-Álvarez Lucía , Thomson Michael M.
Published: June 2021

Circulating recombinant forms (CRFs) contribute substantially to the HIV-1 pandemic. Among 105 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here we find that it belongs to a larger cluster incorporating 39 viruses collected in 7 countries from 3 continents, 22 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). This BF cluster comprises three major subclusters, two associated with Bolivian and one with Peruvian individuals. Near full-length genome sequence analyses of nine viruses, collected in Spain, Bolivia, and Peru, revealed coincident BF mosaic structures, with 13 breakpoints, 6 and 7 of which coincided with CRF12_BF and CRF17_BF, respectively. In a phylogenetic tree, they grouped in a clade closely related to these CRFs, and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allowed to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1986. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF.
Citizen science and niche modeling to track and forecast the expansion of the brown marmorated stinkbug Halyomorpha halys (Stål, 1855)
Authors: Streito Jean-Claude , Chartois Marguerite , Pierre Éric , Dusoulier François , Armand Jean-Marc , Gaudin Jonathan , Rossi Jean-Pierre
Published: June 2021

Halyomorpha halys (Stål, 1855), the Brown Marmorated StinkBug (BMSB) is a highly successful invasive species native to eastern Asia that managed to spread into North America and Europe in recent decades. We set up a citizen science survey to monitor BMSB expansion in France in 2012 and analyzed the data it yielded between 2012 and 2019 to examine the local expansion of the insect. These data were gathered with occurrences form various sources (GBIF, literature) to calibrate a species niche model and assess potential current BMSB range. We evaluated the potential changes to the BMSB range due to climate change by projecting the model according to 6 global circulation models (GCM) and the shared socio-economic pathways SSP245 in two time periods 2021–2040 and 2041–2060. Citizen science allowed to track BMSB expansion in France and provided information about its phenology and its habitat preferences. The model highlighted the potential for further range expansion in Europe and illustrated the impact of climate change. These results could help managing the current BMSB invasion and the framework of this survey could contribute to a better preparedness of phytosanitary authorities either for the BMSB or other invasive pests.
Immunological and hematological outcomes following protracted low dose/low dose rate ionizing radiation and simulated microgravity
Authors: Paul Amber M. , Overbey Eliah G. , da Silveira Willian A. , Szewczyk Nathaniel , Nishiyama Nina C. , Pecaut Michael J. , Anand Sulekha , Galazka Jonathan M. , Mao Xiao Wen
Published: June 2021

Using a ground-based model to simulate spaceflight [21-days of single-housed, hindlimb unloading (HLU) combined with continuous low-dose gamma irradiation (LDR, total dose of 0.04 Gy)], an in-depth survey of the immune and hematological systems of mice at 7-days post-exposure was performed. Collected blood was profiled with a hematology analyzer and spleens were analyzed by whole transcriptome shotgun sequencing (RNA-sequencing). The results revealed negligible differences in immune differentials. However, hematological system analyses of whole blood indicated large disparities in red blood cell differentials and morphology, suggestive of anemia. Murine Reactome networks indicated majority of spleen cells displayed differentially expressed genes (DEG) involved in signal transduction, metabolism, cell cycle, chromatin organization, and DNA repair. Although immune differentials were not changed, DEG analysis of the spleen revealed expression profiles associated with inflammation and dysregulated immune function persist to 1-week post-simulated spaceflight. Additionally, specific regulation pathways associated with human blood disease gene orthologs, such as blood pressure regulation, transforming growth factor-β receptor signaling, and B cell differentiation were noted. Collectively, this study revealed differential immune and hematological outcomes 1-week post-simulated spaceflight conditions, suggesting recovery from spaceflight is an unremitting process.