bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the Powder-Spreading Process of Walnut Shell/Co-PES Biomass Composite Powder in Additive Manufacturing
Authors: Yu Yueqiang , Ma Tingang , Wang Suling , Jiang Minzheng , Gao Sheng , Guo Yanling , Jiang Ting , Doumbia Bakary S. , Yan Bo , Shen Shaorui
Published: June 2023

Powder laying is a necessary procedure during powder bed additive manufacturing (PBAM), and the quality of powder bed has an important effect on the performance of products. Because the powder particle motion state during the powder laying process of biomass composites is difficult to observe, and the influence of the powder laying process parameters on the quality of the powder bed is still unclear, a simulation study of the biomass composite powder laying process during powder bed additive manufacturing was conducted using the discrete element method. A discrete element model of walnut shell/Co-PES composite powder was established using the multi-sphere unit method, and the powder-spreading process was numerically simulated using two different powder spreading methods (rollers/scrapers). The results showed that the quality of powder bed formed by roller laying was better than that formed by scrapers with the same powder laying speed and powder laying thickness. For both of the two different spreading methods, the uniformity and density of the powder bed decreased as spreading speed increased, although the spreading speed had a more important influence on scraper spreading compared to roller spreading. As powder laying thickness increased, the powder bed formed by the two different powder laying methods became more uniform and denser. When the powder laying thickness was less than 110μm, the particles were easily blocked at the powder laying gap and are pushed out of the forming platform, forming many voids, and decreasing the powder bed’s quality. When the powder thickness was greater than 140 μm, the uniformity and density of the powder bed increased gradually, the number of voids decreased, and the quality of the powder bed improved.
The Primary Irradiation Damage of Hydrogen-Accumulated Nickel: An Atomistic Study
Authors: Yuan Xiaoting , Huang Hai , Zhong Yinghui , Cai Bin , Liu Zhongxia , Peng Qing
Published: June 2023

Nickel-based alloys have demonstrated significant promise as structural materials for Gen-IV nuclear reactors. However, the understanding of the interaction mechanism between the defects resulting from displacement cascades and solute hydrogen during irradiation remains limited. This study aims to investigate the interaction between irradiation-induced point defects and solute hydrogen on nickel under diverse conditions using molecular dynamics simulations. In particular, the effects of solute hydrogen concentrations, cascade energies, and temperatures are explored. The results show a pronounced correlation between these defects and hydrogen atoms, which form clusters with varying hydrogen concentrations. With increasing the energy of a primary knock-on atom (PKA), the number of surviving self-interstitial atoms (SIAs) also increases. Notably, at low PKA energies, solute hydrogen atoms impede the clustering and formation of SIAs, while at high energies, they promote such clustering. The impact of low simulation temperatures on defects and hydrogen clustering is relatively minor. High temperature has a more obvious effect on the formation of clusters. This atomistic investigation offers valuable insights into the interaction between hydrogen and defects in irradiated environments, thereby informing material design considerations for next-generation nuclear reactors.
Study on Sound-Insulation Performance of an Acoustic Metamaterial of Air-Permeable Multiple-Parallel-Connection Folding Chambers by Acoustic Finite Element Simulation
Authors: Peng Wenqiang , Bi Shaohua , Shen Xinmin , Yang Xiaocui , Yang Fei , Wang Enshuai
Published: June 2023

In order to achieve a balance between sound insulation and ventilation, a novel acoustic metamaterial of air-permeable multiple-parallel-connection folding chambers was proposed in this study that was based on Fano-like interference, and its sound-insulation performance was investigated through acoustic finite element simulation. Each layer of the multiple-parallel-connection folding chambers consisted of a square front panel with many apertures and a corresponding chamber with many cavities, which were able to extend both in the thickness direction and in the plane direction. Parametric analysis was conducted for the number of layers nl and turns nt, the thickness of each layer L2, the inner side lengths of the helical chamber a1, and the interval s among the various cavities. With the parameters of nl = 10, nt = 1, L2 = 10 mm, a1 = 28 mm, and s = 1 mm, there were 21 sound-transmission-loss peaks in the frequency range 200–1600 Hz, and the sound-transmission loss reached 26.05 dB, 26.85 dB, 27.03 dB, and 33.6 dB at the low frequencies 468 Hz, 525 Hz, 560 Hz, and 580 Hz, respectively. Meanwhile, the corresponding open area for air passage reached 55.18%, which yielded a capacity for both efficient ventilation and high selective-sound-insulation performance.
Some Magnetic Properties and Magnetocaloric Effects in the High-Temperature Antiferromagnet YbCoC2
Authors: Salamatin Denis Alexandrovich , Krasnorussky Vladimir Nikolaevich , Magnitskaya Mariya Viktorovna , Semeno Alexei Valeryevich , Bokov Alexander Vladimirovich , Velichkov Atanas , Surowiec Zbigniew , Tsvyashchenko Anatoly Vasilyevich
Published: June 2023

The YbCoC2 compound, which crystallizes in a base-centered orthorhombic unit cell in the Amm2 space group CeNiC2 structure, is unique among Yb-based compounds due to the highest magnetic ordering temperature of TN=27 K. Magnetization measurements have made it possible to plot the H-T magnetic phase diagram and determine the magnetocaloric effect of this recently discovered high-temperature heavy-fermion compound, YbCoC2. YbCoC2 undergoes spin transformation to the spin-polarized state through a metamagnetic transition in an external magnetic field. The transition is found to be of the first order. The dependencies of magnetic entropy change ΔSm(T)—have segments with positive and negative magnetocaloric effects for ΔH≤6 T. For ΔH=9 T, the magnetocaloric effect becomes positive, with a maximum ΔSm(T) value of 4.1 J (kg K)−1 at TN and a refrigerant capacity value of 56.6 J kg−1.
Band Structure Evolution during Reversible Interconversion between Dirac and Standard Fermions in Organic Charge-Transfer Salts
Authors: Oka Ryuhei , Ohara Keishi , Konishi Kensuke , Yamane Ichiro , Shimada Toshihiro , Naito Toshio
Published: June 2023

Materials containing Dirac fermions (DFs) have been actively researched because they often alter electrical and magnetic properties in an unprecedented manner. Although many studies have suggested the transformation between standard fermions (SFs) and DFs, the non-availability of appropriate samples has prevented the observation of the transformation process. We observed the interconversion process of DFs and SFs using organic charge-transfer (CT) salts. The samples are unique in that the constituents (the donor D and acceptor A species) are particularly close to each other in energy, leading to the temperature- and D-A-combination-sensitive CT interactions in the solid states. The three-dimensional weak D–A CT interactions in low-symmetry crystals induced the continuous reshaping of flat-bottomed bands into Dirac cones with decreasing temperature; this is a characteristic shape of bands that converts the behavior of SFs into that of DFs. Based on the first-principles band structures supported by the observed electronic properties, round-apex-Dirac-cone-like features appear and disappear with temperature variation. These band-structure snapshots are expected to add further detailed understanding to the related research fields.
Optimal Robust Tracking Control of Injection Velocity in an Injection Molding Machine
Authors: Wu Guoshen , Ren Zhigang , Li Jiajun , Wu Zongze
Published: June 2023

Injection molding is a critical component of modern industrial operations, and achieving fast and stable control of injection molding machines (IMMs) is essential for producing high-quality plastic products. This paper focuses on solving an optimal tracking control problem of the injection velocity that arises in a typical nonlinear IMM. To this end, an efficient optimal robust controller is proposed and designed. The nonlinear injection velocity servo system is first approximately linearized at iteration points using the first-order Taylor expansion approach. Then, at each time node in the optimization process, the relevant algebraic Riccati equation is introduced, and the solution is used to construct an optimal robust feedback controller. Furthermore, a rigorous Lyapunov theorem analysis is employed to demonstrate the global stability properties of the proposed feedback controller. The results from numerical simulations show that the proposed optimal robust control strategy can successfully and rapidly achieve the best tracking of the intended injection velocity trajectory within a given time.
On Correspondences between Feedforward Artificial Neural Networks on Finite Memory Automata and Classes of Primitive Recursive Functions
Authors: Kulyukin Vladimir A.
Published: June 2023

When realized on computational devices with finite quantities of memory, feedforward artificial neural networks and the functions they compute cease being abstract mathematical objects and turn into executable programs generating concrete computations. To differentiate between feedforward artificial neural networks and their functions as abstract mathematical objects and the realizations of these networks and functions on finite memory devices, we introduce the categories of general and actual computabilities and show that there exist correspondences, i.e., bijections, between functions computable by trained feedforward artificial neural networks on finite memory automata and classes of primitive recursive functions.
Observer-Based Fuzzy Control of Uncertain Nonlinear Singular Systems under Multi-Performance Requirements
Authors: Chang Wen-Jer , Huang Yu-Min , Lin Yann-Horng
Published: June 2023

This paper discusses an observer-based fuzzy control problem for uncertain nonlinear singular systems under Multi-Performance Requirements (MPRs). The approach used in the paper is to model the system using a Takagi–Sugeno (T-S) fuzzy model that can be analyzed using linear control theories. The proposed control scheme is based on the Parallel Distributed Compensation (PDC) approach and Proportional Derivative (PD) control scheme. The goal is to design an observer-based fuzzy controller that achieves stability of the system and also satisfies the Guarantee Cost Control (GCC) constraint while maintaining a desired passive constraint. The stability analysis is performed using Lyapunov theory, and the sufficient conditions are transformed into a Linear Matrix Inequality (LMI) form using a Shur Complement, free-weighting matrix method and Singular Value Decomposition (SVD) techniques. The LMI conditions are then solved using convex optimization algorithms. Finally, the proposed control method is validated using a bio-economic system to demonstrate its effectiveness.
Parameter Estimation for Nonlinear Diffusion Problems by the Constrained Homotopy Method
Authors: Liu Tao , Ding Zijian , Yu Jiayuan , Zhang Wenwen
Published: June 2023

This paper studies a parameter estimation problem for the non-linear diffusion equation within multiphase porous media flow, which has important applications in the field of oil reservoir simulation. First, the given problem is transformed into an optimization problem by using optimal control framework and the constraints such as well logs, which can restrain noise and improve the quality of inversion, are introduced. Then we propose the widely convergent homotopy method, which makes natural use of constraints and incorporates Tikhonov regularization. The effectiveness of the proposed approach is demonstrated on illustrative examples.
Variability in Macro- and Micronutrients of 15 Rarely Researched Microalgae
Authors: Sandgruber Fabian , Gielsdorf Annekathrin , Schenz Benjamin , Müller Sandra Marie , Schwerdtle Tanja , Lorkowski Stefan , Griehl Carola , Dawczynski Christine
Published: June 2023

Microalgae have enormous potential for human nutrition, yet the European Commission has authorized the consumption of only eleven species. Strains of fifteen rarely researched microalgae from two kingdoms were screened regarding their nutritional profile and value for human health in two cultivation phases. Contents of protein, fiber, lipids, fatty acids, minerals, trace elements and heavy metals were determined. In the growth phase, microalgae accumulated more arginine, histidine, ornithine, pure and crude protein, Mg, Mn, Fe and Zn and less Ni, Mo and I2 compared to the stationary phase. Higher contents of total fat, C14:0, C14:1n5, C16:1n7, C20:4n6, C20:5n3 and also As were observed in microalgae from the chromista kingdom in comparison to microalgae from the plantae kingdom (p < 0.05). Conversely, the latter had higher contents of C20:0, C20:1n9 and C18:3n3 as well as Ca and Pb (p < 0.05). More precisely, Chrysotila carterae appeared to have great potential for human nutrition because of its high nutrient contents such as fibers, carotenoids, C20:6n3, Mg, Ca, Mn, Fe, Se, Zn, Ni, Mo and I2. In summary, microalgae may contribute to a large variety of nutrients, yet the contents differ between kingdoms, cultivation phases and also species.
Brolucizumab Intravitreal Injections for Wet Age-Related Macular Degeneration: Real-Life Study on a Cohort of Italian Patients
Authors: Gesualdo Carlo , Rossi Settimio , Iodice Clemente Maria , Guarino Francesco , Petrella Mariachiara , D’Agostino Fabiana Anna , Perrotta Raffaele , Simonelli Francesca
Published: June 2023

Background and Objectives: To report the real-life Brolucizumab therapeutical outcomes of treatment-naïve and non-treatment-naïve eyes with neovascular age-related macular degeneration (nAMD) and to analyze the incidence of therapy-related adverse events. Materials and Methods: A total of 56 eyes of 54 patients diagnosed with nAMD were retrospectively evaluated over a 3-month follow-up. Naïve eyes received a 3-month loading phase, whereas non-naïve eyes were treated with one intravitreal injection + ProReNata scheme. The main outcome measures were best-corrected visual acuity (BCVA) and central retinal thickness (CRT) change. In addition, patients were stratified on the basis of fluid accumulation site, whether intra-retinal (IRF), sub-retinal (SRF), or sub-retinal pigmented epithelium (SRPE), to separately assess the eventual BCVA change in each subgroup. Finally, the incidence of ocular adverse events was evaluated. Results: In naïve eyes, a significant improvement of BCVA (LogMar) was observed at all timepoints from baseline (1 month-Mean Difference (MD): −0.13; 2 months MD: −0.17; 3 months MD: −0.24). In non-naïve eyes, a significant mean change was observed at all timepoints, with the exception of 1-month follow-up (2 months MD: −0.08; 3 months MD: −0.05). CRT significantly changed in both groups at all timepoints at a similar pace within the first two months, with naïve eyes displaying a larger overall thickness decrease at the end of the follow-up (Group 1 = MD: −123.91 µm; Group 2 = MD: −110.33 µm). With respect to the location of the edema, a significant BCVA change was observed in naïve patients with fluid in all three sites at the end of the follow-up (SRPE = MD: −0.13 (p = 0.043); SR = MD: −0.15 (p = 0.019); IR = MD: −0.19 (p = 0.041). Non-naïve patients exhibited significant mean BCVA changes only with respect to SR and IR fluid presence (SRPE = MD: −0.13 (p = 0.152); SR = MD: −0.15 (p = 0.007); IR = MD: −0.06 (p = 0.011). One naïve patient experienced acute-onset anterior and intermediate uveitis which completely resolved after therapy. Conclusions: Brolucizumab was demonstrated to be a safe and efficient alternative in improving both the anatomical and functional parameters of eyes with nAMD in this small, uncontrolled, series of patients.
Evaluation of Paraspinal Muscle Degeneration on Pain Relief after Percutaneous Epidural Adhesiolysis in Patients with Degenerative Lumbar Spinal Disease
Authors: Kang Misun , Kim Shin Hyung , Jo Minju , Jung Hyun Eom , Bae Jungbin , Kim Hee Jung
Published: June 2023

Background and Objectives: The analgesic effectiveness of epidural adhesiolysis may be influenced by morphological changes in the paraspinal muscles, particularly in elderly patients. The objective of this study was to assess whether the cross-sectional area or fatty infiltration of the paraspinal muscles impacts the treatment outcomes of epidural adhesiolysis. Materials and Methods: The analysis included a total of 183 patients with degenerative lumbar disease who underwent epidural adhesiolysis. Good analgesia was defined as a reduction in pain score of ≥30% at the 6-month follow up. We measured the cross-sectional area and fatty infiltration rate of the paraspinal muscles and divided the study population into age groups (≥65 years and <65 years). Variables were compared between the good and poor analgesia groups. Results: The results revealed that elderly patients experienced poorer analgesic outcomes as the rate of fatty infiltration in the paraspinal muscles increased (p = 0.029), predominantly in female patients. However, there was no correlation between the cross-sectional area and the analgesic outcome in patients younger than or older than 65 years (p = 0.397 and p = 0.349, respectively). Multivariable logistic regression analysis indicated that baseline pain scores < 7 (Odds Ratio (OR) = 4.039, 95% Confidence Interval (CI) = 1.594–10.233, p = 0.003), spondylolisthesis (OR = 4.074, 95% CI = 1.144–14.511, p = 0.030), and ≥ 50% fatty infiltration of the paraspinal muscles (OR = 6.576, 95% CI = 1.300–33.268, p = 0.023) were significantly associated with poor outcomes after adhesiolysis in elderly patients. Conclusions: Fatty degeneration of paraspinal muscles is correlated with inferior analgesic outcomes following epidural adhesiolysis in elderly patients, but not in young and middle-aged patients. The cross-sectional area of the paraspinal muscles is not associated with pain relief after the procedure.
Antiseizure Medication-Induced Alopecia: A Literature Review
Authors: Pitton Rissardo Jamir , Fornari Caprara Ana Leticia , Casares Maritsa , Skinner Holly J. , Hamid Umair
Published: June 2023

Background: Adverse effects of antiseizure medications (ASMs) remain one of the major causes of non-adherence. Cosmetic side effects (CSEs) are among the most commonly reported side effects of ASMs. In this context, alopecia is one of the CSEs that has a high intolerance rate leading to poor therapeutical compliance. Methods: We performed a literature review concerning alopecia as a secondary effect of ASMs. Results: There are 1656 individuals reported with ASM-induced alopecia. Valproate (983), lamotrigine (355), and carbamazepine (225) have been extensively reported. Other ASMs associated with alopecia were cenobamate (18), levetiracetam (14), topiramate (13), lacosamide (7), vigabatrin (6), phenobarbital (5), gabapentin (5), phenytoin (4), pregabalin (4), eslicarbazepine (3), brivaracetam (2), clobazam (2), perampanel (2), trimethadione (2), rufinamide (2), zonisamide (2), primidone (1), and tiagabine (1). There were no reports of oxcarbazepine and felbamate with drug-induced alopecia. Hair loss seen with ASMs was diffuse and non-scarring. Telogen effluvium was the most common cause of alopecia. A characteristic feature was the reversibility of alopecia after ASM dose adjustment. Conclusions: Alopecia should be considered one important adverse effect of ASMs. Patients reporting hair loss with ASM therapy should be further investigated, and specialist consultation is recommended.
Recent Advanced Synthesis Strategies for the Nanomaterial-Modified Proton Exchange Membrane in Fuel Cells
Authors: Chandra Kishore Somasundaram , Perumal Suguna , Atchudan Raji , Alagan Muthulakshmi , Wadaan Mohammad Ahmad , Baabbad Almohannad , Manoj Devaraj
Published: June 2023

Hydrogen energy is converted to electricity through fuel cells, aided by nanostructured materials. Fuel cell technology is a promising method for utilizing energy sources, ensuring sustainability, and protecting the environment. However, it still faces drawbacks such as high cost, operability, and durability issues. Nanomaterials can address these drawbacks by enhancing catalysts, electrodes, and fuel cell membranes, which play a crucial role in separating hydrogen into protons and electrons. Proton exchange membrane fuel cells (PEMFCs) have gained significant attention in scientific research. The primary objectives are to reduce greenhouse gas emissions, particularly in the automotive industry, and develop cost-effective methods and materials to enhance PEMFC efficiency. We provide a typical yet inclusive review of various types of proton-conducting membranes. In this review article, special focus is given to the distinctive nature of nanomaterial-filled proton-conducting membranes and their essential characteristics, including their structural, dielectric, proton transport, and thermal properties. We provide an overview of the various reported nanomaterials, such as metal oxide, carbon, and polymeric nanomaterials. Additionally, the synthesis methods in situ polymerization, solution casting, electrospinning, and layer-by-layer assembly for proton-conducting membrane preparation were analyzed. In conclusion, the way to implement the desired energy conversion application, such as a fuel cell, using a nanostructured proton-conducting membrane has been demonstrated.
Evaluating Surface Quality of Inconel 617 by Employing Deep Cryogenically Treated Electrodes in Surfactant-Added Dielectrics of Transformer Oil
Authors: Ishfaq Kashif , Sana Muhammad , Mahmood Muhammad Arif , Anwar Saqib , Waseem Muhammad Umair
Published: June 2023

Over the past few decades, better surface quality has remained of great interest to researchers. It deteriorates the fatigue life of the workpiece. The criticality arises when a material of greater strength is selected to work in high-temperature areas such as nickel (Ni)-based superalloys, categorically Inconel 617. Conventional machining operations are not the best choice for the machining of this alloy because of its low density and greater strength. Therefore, electric discharge machining (EDM) is generally engaged. Still, there is a great necessity to make a more reliable surface using EDM, which performs better even in harsh working areas. Therefore, this study examined the potential of deep-cryogenically treated electrodes under the modified dielectrics of transformer oil in the said context, which has not been discussed so far. A set of 30 experiments was performed, designed using the full factorial technique. Deep-cryogenically treated electrodes provided better surface quality in comparison to the non-treated electrodes. Amongst the deep-cryogenically treated electrodes, brass performed outstandingly and provided the lowest value of surface roughness (SR), 6.65 µm, in the modified dielectric of transformer oil with Span 80. The surface finish of deep-cryogenically treated brass is 28.72% better compared to the average value of the overall deep-cryogenically treated electrodes. The lowest value of SR (8.35 µm) was gained by engaging a non-cryogenically treated Cu electrode with a T-20–transformer oil-modified dielectric. The said value of SR is 17.7% better than the highest value of SR achieved in the case of S-80–transformer oil with a non-cryogenically treated Cu electrode.
Effect of Nanosized Ni Reinforcements on the Structure of the Sn-3.0Ag-0.5Cu Alloy in Liquid and After-Reflow Solid States
Authors: Yakymovych Andriy , Shtablavyi Ihor
Published: June 2023

The Sn-Ag-Cu (SAC) alloy family is commonly used in lead-free solders employed in the electronics industry, for instance, SAC305, SAC387, SAC405, etc. However, the trend in manufacturing small electronic products and device miniaturization faces some disadvantages in terms of mechanical properties and their higher melting temperatures compared to Pb-Sn solders, prompting new research relating to the reinforcement of existing SAC solders. The current study presents structural features of nanocomposite (Sn-3.0Ag-0.5Cu)100−x(nanoNi)x solders with 0.5 wt.%, 1.0 wt.%, and 2.0 wt.% Ni. Structural analysis of the investigated samples were performed by means of X-ray diffraction in a liquid state and scanning electron microscopy (SEM). SEM showed the mutual substitution of Ni and Cu atoms in the Cu6Sn5 and Ni3Sn4 phases, respectively. The performed structural studies in liquid and solid states provided essential information concerning the structural transformations of liquid Sn-3.0Ag-0.5Cu alloys caused by minor additions of nanosized Ni powder. The melting point and degree of undercooling of the samples were investigated by DTA analysis.
Holographic Fabrication of 3D Moiré Photonic Crystals Using Circularly Polarized Laser Beams and a Spatial Light Modulator
Authors: Hurley Noah , Kamau Steve , Cui Jingbiao , Lin Yuankun
Published: June 2023

A moiré photonic crystal is an optical analog of twisted graphene. A 3D moiré photonic crystal is a new nano-/microstructure that is distinguished from bilayer twisted photonic crystals. Holographic fabrication of a 3D moiré photonic crystal is very difficult due to the coexistence of the bright and dark regions, where the exposure threshold is suitable for one region but not for the other. In this paper, we study the holographic fabrication of 3D moiré photonic crystals using an integrated system of a single reflective optical element (ROE) and a spatial light modulator (SLM) where nine beams (four inner beams + four outer beams + central beam) are overlapped. By modifying the phase and amplitude of the interfering beams, the interference patterns of 3D moiré photonic crystals are systemically simulated and compared with the holographic structures to gain a comprehensive understanding of SLM-based holographic fabrication. We report the holographic fabrication of phase and beam intensity ratio-dependent 3D moiré photonic crystals and their structural characterization. Superlattices modulated in the z-direction of 3D moiré photonic crystals have been discovered. This comprehensive study provides guidance for future pixel-by-pixel phase engineering in SLM for complex holographic structures.
A Hemodynamic Pulse Wave Simulator Designed for Calibration of Local Pulse Wave Velocities Measurement for Cuffless Techniques
Authors: Guo Cheng-Yan , Perng Jau-Woei , Chen Li-Ching , Hsieh Tung-Li
Published: June 2023

Objective: Devices for cuffless blood pressure (BP) measurement have become increasingly widespread in recent years. Non-invasive continuous BP monitor (BPM) devices can diagnose potential hypertensive patients at an early stage; however, these cuffless BPMs require more reliable pulse wave simulation equipment and verification methods. Therefore, we propose a device to simulate human pulse wave signals that can test the accuracy of cuffless BPM devices using pulse wave velocity (PWV). Methods: We design and develop a simulator capable of simulating human pulse waves comprising an electromechanical system to simulate the circulatory system and an arm model-embedded arterial phantom. These parts form a pulse wave simulator with hemodynamic characteristics. We use a cuffless device for measuring local PWV as the device under test to measure the PWV of the pulse wave simulator. We then use a hemodynamic model to fit the cuffless BPM and pulse wave simulator results; this model can rapidly calibrate the cuffless BPM’s hemodynamic measurement performance. Results: We first used multiple linear regression (MLR) to generate a cuffless BPM calibration model and then investigated differences between the measured PWV with and without MLR model calibration. The mean absolute error of the studied cuffless BPM without the MLR model is 0.77 m/s, which improves to 0.06 m/s when using the model for calibration. The measurement error of the cuffless BPM at BPs of 100–180 mmHg is 1.7–5.99 mmHg before calibration, which decreases to 0.14–0.48 mmHg after calibration. Conclusion: This study proposes a design of a pulse wave simulator based on hemodynamic characteristics and provides a standard performance verification method for cuffless BPMs that requires only MLR modeling on the cuffless BPM and pulse wave simulator. The pulse wave simulator proposed in this study can be used to quantitively assess the performance of cuffless BPMs. The proposed pulse wave simulator is suitable for mass production for the verification of cuffless BPMs. As cuffless BPMs become increasingly widespread, this study can provide performance testing standards for cuffless devices.
p25alpha Domain-Containing Proteins of Apicomplexans and Related Taxa
Authors: Orosz Ferenc
Published: June 2023

TPPP (tubulin polymerization promoting protein)-like proteins contain one or more p25alpha (Pfam05517) domains. TPPP-like proteins occur in different types as determined by their length (e.g., long-, short-, truncated-, and fungal-type TPPP) and include the protein apicortin, which possesses another domain, doublecortin (DCX, Pfam 03607). These various TPPP-like proteins are found in various phylogenomic groups. In particular, short-type TPPPs and apicortin are well-represented in the Myzozoa, which include apicomplexans and related taxa, chrompodellids, dinoflagellates, and perkinsids. The long-, truncated-, and fungal-type TPPPs are not found in the myzozoans. Apicortins are found in all apicomplexans except one piroplasmid species, present in several other myzozoans, and seem to be correlated with the conoid and apical complex. Short-type TPPPs are predominantly found in myzozoans that have flagella, suggesting a role in flagellum assembly or structure.
Endophytes and Plant Extracts as Potential Antimicrobial Agents against Candidatus Liberibacter Asiaticus, Causal Agent of Huanglongbing
Authors: Dominguez Jessica , Jayachandran Krishnaswamy , Stover Ed , Krystel Joseph , Shetty Kateel G.
Published: June 2023

Huanglongbing (HLB), also known as citrus greening, is an insidious disease in citrus and has become a threat to the sustainability of the citrus industry worldwide. In the U.S., Candidatus Liberibacter asiaticus (CLas) is the pathogen that is associated with HLB, an unculturable, phloem-limited bacteria, vectored by the Asian Citrus Psyllid (ACP, Diaphorina citri). There is no known cure nor treatment to effectively control HLB, and current control methods are primarily based on the use of insecticides and antibiotics, where effectiveness is limited and may have negative impacts on beneficial and non-target organisms. Thus, there is an urgent need for the development of effective and sustainable treatment options to reduce or eliminate CLas from infected trees. In the present study, we screened citrus-derived endophytes, their cell-free culture supernatants (CFCS), and crude plant extracts for antimicrobial activity against two culturable surrogates of CLas, Sinorhizobium meliloti and Liberibacter crescens. Candidates considered high-potential antimicrobial agents were assessed directly against CLas in vitro, using a propidium monoazide–based assay. As compared to the negative controls, statistically significant reductions of viable CLas cells were observed for each of the five bacterial CFCS. Subsequent 16S rRNA gene sequencing revealed that each of the five bacterial isolates were most closely related to Bacillus amyloliquefaciens, a species dominating the market of biological control products. As such, the aboveground endosphere of asymptomatic survivor citrus trees, grown in an organic orchard, were found to host bacterial endophytes capable of effectively disrupting CLas cell membranes. These results concur with the theory that native members of the citrus microbiome play a role in the development of HLB. Here, we identify five strains of Bacillus amyloliquefaciens demonstrating notable potential to be used as sources of novel antimicrobials for the sustainable management of HLB.
Molecular Investigation Confirms Myotis Genus Bats as Common Hosts of Polychromophilus in Brazil
Authors: Mathias Bruno da Silva , Minozzo Guilherme Augusto , Biondo Alexander Welker , Costa Jaciara de Oliveira Jorge , Soares Herbert Sousa , Marcili Arlei , Guimarães Lilian de Oliveira , Anjos Carolina Clares dos , Santos Andrea Pires Dos , Riediger Irina Nastassja , Fecchio Alan , Bueno Marina Galvão , Pinho João Batista , Kirchgatter Karin
Published: June 2023

Plasmodium spp. and some other blood parasites belonging to the order Haemosporida are the focus of many epidemiological studies worldwide. However, haemosporidian parasites from wild animals are largely neglected in scientific research. For example, Polychromophilus parasites, which are exclusive to bats, are described in Europe, Asia, Africa, and Oceania, but little is known about their presence and genetic diversity in the New World. In this study, 224 samples of bats from remaining fragments of the Atlantic Forest and Pantanal biomes, as well as urbanized areas in southern and southeastern Brazil, were analyzed for the presence of haemosporidian parasites by PCR of the mitochondrial gene that encodes cytochrome b (cytb). The PCR fragments of the positive samples were sequenced and analyzed by the Bayesian inference method to reconstruct the phylogenetic relationships between Polychromophilus parasites from bats in Brazil and other countries. Sequences from Brazilian lineages of Polychromophilus were recovered in a clade with sequences from Polychromophilus murinus and close to the one Polychromophilus sequence obtained in Panama, the only available sequence for the American continent. This clade was restricted to bats of the family Vespertilionidae and distinct from Polychromophilus melanipherus, a parasite species mainly found in bats of the family Miniopteridae. The detection of Polychromophilus and the genetic proximity to P. murinus were further confirmed with the amplification of two other genes (clpc and asl). We also found a Haemosporida parasite sequence in a sample of Noctilio albiventris collected in the Pantanal biome, which presents phylogenetic proximity with avian Haemoproteus sequences. Morphological and molecular studies are still needed to conclude and describe the Polychromophilus species in Brazilian Myotis bats in more detail and to confirm Haemoproteus parasites in bats. Nevertheless, these molecular results in Brazilian bats confirm the importance of studying these neglected genera.
Upregulation of ica Operon Governs Biofilm Formation by a Coagulase-Negative Staphylococcus caprae
Authors: Oknin Hilla , Kroupitski Yulia , Shemesh Moshe , Blum Shlomo
Published: June 2023

Staphylococcus caprae is a Gram-positive, coagulase-negative staphylococci (CoNS), which appears as commensal in the skin, as well as a prevalent mastitis pathogen of goats. Occasionally, it is also associated with infections in humans. Biofilm formation has been identified as a putative virulence factor in S. caprae. Biofilms are multicellular communities protected by a self-produced extracellular matrix (ECM), which facilitates the resistance of bacterial cells to antimicrobial treatments. The ECM is constructed by exopolysaccharides, including the major exopolysaccharide—polysaccharide intercellular adhesion (PIA), regulated by the ica operon in Staphylococcus species. The aim of this study was to characterize the expression of the ica operon in relation to biofilm formation in S. caprae. Results showed that within a few hours of growth, S. caprae could adhere to polystyrene surfaces, start to accumulate, and form biofilm. Peak biofilm biomass and maturation were reached after 48 h, followed by a reduction in biomass after 72 h. Confocal laser scanning microscopy showed the expression of matrix-associated proteins and polysaccharides at various time points. The expression dynamics of the ica operon were investigated using real-time reverse transcriptase PCR (RT)-qPCR, which showed elevated expression during the early stages of biofilm formation and subsequent downregulation throughout the biofilm aging process. In conclusion, our results show that the ica operon is essential in regulating biofilm formation in S. caprae, similar to other Staphylococcus species. Furthermore, the robustness of the observed biofilm phenotype could account for the successful intramammary colonization and may explain disease persistence caused by this pathogenic bacterium.
Microbiome-Related and Infection Control Approaches to Primary and Secondary Prevention of Clostridioides difficile Infections
Authors: McFarland Lynne V. , Goldstein Ellie J. C. , Kullar Ravina
Published: June 2023

Clostridioides difficile infections (CDIs) have decreased in the past years, but since 2021, some hospitals have reported an increase in CDI rates. CDI remains a global concern and has been identified as an urgent threat to healthcare. Although multiple treatment options are available, prevention strategies are more limited. As CDI is an opportunistic infection that arises after the normally protective microbiome has been disrupted, preventive measures aimed at restoring the microbiome have been tested. Our aim is to update the present knowledge on these various preventive strategies published in the past five years (2018–2023) to guide clinicians and healthcare systems on how to best prevent CDI. A literature search was conducted using databases (PubMed, Google Scholar, and clinicaltrials.gov) for phase 2–3 clinical trials for the primary or secondary prevention of CDI and microbiome and probiotics. As the main factor for Clostridium difficile infections is the disruption of the normally protective intestinal microbiome, strategies aimed at restoring the microbiome seem most rational. Some strains of probiotics, the use of fecal microbial therapy, and live biotherapeutic products offer promise to fill this niche; although, more large randomized controlled trials are needed that document the shifts in the microbiome population.
Functional Analysis of Novel alkB Genes Encoding Long-Chain n-Alkane Hydroxylases in Rhodococcus sp. Strain CH91
Authors: Xiang Wei , Hong Shan , Xue Yanfen , Ma Yanhe
Published: June 2023

Rhodococcus sp. strain CH91 is capable of utilizing long-chain n-alkanes as the sole carbon source. Two new genes (alkB1 and alkB2) encoding AlkB-type alkane hydroxylase were predicted by its whole-genome sequence analysis. The purpose of this study was to elucidate the functional role of alkB1 and alkB2 genes in the n-alkane degradation of strain CH91. RT-qPCR analyses revealed that the two genes were induced by n-alkanes ranging from C16 to C36 and the expression of the alkB2 gene was up-regulated much higher than that of alkB1. The knockout of the alkB1 or alkB2 gene in strain CH91 resulted in the obvious reduction of growth and degradation rates on C16-C36 n-alkanes and the alkB2 knockout mutant exhibited lower growth and degradation rate than the alkB1 knockout mutant. When gene alkB1 or alkB2 was heterologously expressed in Pseudomonas fluorescens KOB2Δ1, the two genes could restore its alkane degradation activity. These results demonstrated that both alkB1 and alkB2 genes were responsible for C16-C36 n-alkanes’ degradation of strain CH91, and alkB2 plays a more important role than alkB1. The functional characteristics of the two alkB genes in the degradation of a broad range of n-alkanes make them potential gene candidates for engineering the bacteria used for bioremediation of petroleum hydrocarbon contaminations.
Spatial Heterogeneity of Rare Earth Elements: Implications for the Topsoil of Regional Ion-Adsorption Type Rare Earth Deposit Areas in Southern China
Authors: Chen Haixia , Chen Lingkang , Zhang Lian , Guo Min
Published: June 2023

The migration and spatial distribution characteristics of topsoil rare earth elements (REEs) are significant for the risk assessment of the external environment. However, the spatial distribution of REEs in the topsoil of ion-adsorption type rare earth element (REE) mining areas is poorly studied. We aimed to determine the differences and control factors of the spatial distribution of REEs in the topsoil of typical rare earth mines in South Jiangxi, South China. Sixty-five topsoil samples and eighteen profile samples were collected and analyzed for their rare earth content to elucidate spatial autocorrelation and heterogeneity using statistical analysis software (IBM SPSS Statistics 26.0.0.0, GS+9.0, and Arcgis10.2.0.3348). Moran index analysis showed that the positive correlation between sampling points was significant within the range of 0–500 m. The best fitting models of the semi-variance variogram were the exponential model, Gaussian model, and spherical model. The sequence of the spatial structure (C0 + C) was Ho > Tb > La > Pr > Nd > Sm > Gd > Tm > Lu > Dy > Er > Yb > Ce > Eu. The spatial fractal distribution pattern was Ho > Tb > Lu > Er > Dy > Yb > Tm > Gd > Ce > La > Eu > Sm > Pr > Nd. This indicated that the light rare earth elements (LREEs) and heavy rare earth elements (HREEs) in the topsoil were significantly different from the other sediments. This study provides new evidence for the environmental quality assessment of the in situ leaching of ionic rare earth ores into the topsoil layer.
Raw Smectite from the Guarapuava–Parana–Brasil Saturated with Copper Ions and Its Properties
Authors: Rocha Maria Lidia M. , Balaba Nayara , Jaerger Silvia , Primo Julia O. , Horsth Dienifer F. L. , Appelt Patrícia , Meneguzzi Daiane , Cunha Mário A. A. , Anaissi Fauze J.
Published: June 2023

In this study, raw smectite (Sm), from the Guarapuava–Parana–Brasil region, was saturated with copper ions (Cu-Sm) by ion exchange and the samples Sm and Cu-Sm were used in crystal violet (CV) adsorption and applied as an antimicrobial and antifungal hybrid pigment. Samples (Sm and Cu-Sm) were used to remove crystal violet (CV) dye from aqueous media, simulating wastewater. Samples after use as adsorbents were characterized and named smectite/adsorbed dye (Sm/Dye) and copper smectite/adsorbed dye (Cu-Sm/Dye); and they were applied as hybrid pigments with antimicrobial action. The Sm and Cu-Sm were characterized by X-ray diffractometry (XRD), energy dispersive X-ray fluorescence (EDXRF), vibrational spectroscopy (FTIR), Zeta potential (ζ), scanning electron microscopy (SEM), and colorimetry (CIE L*a*b*), enabling the identification of the presence of intercalated copper ions and on the smectite surface. The adsorption assays were carried out to evaluate the effects of initial dye concentration and contact time. Tests for application as a hybrid pigment showed good compatibility with commercial white paint being applied on plaster blocks and later photoaging and chemical stability tests were performed in acid and basic environments, both were discussed by colorimetry (CIE L*a*b*), thus being able to relate it to the color variation (∆E). The samples (Sm, Cu-Sm, Sm/Dye, and Cu-Sm/Dye) were dispersed in white paint at 10% and 20% (% w/w) to evaluate the ability to inhibit different microorganisms. The modification with copper ions promoted an increase in the adsorptive capacity relative to the raw smectite and provided antibacterial and antifungal action to the hybrid pigment against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Candida albicans. The Cu-Sm and Cu-Sm/Dye samples showed excellent results against all studied microorganisms and reveal successful materials that can be used in environments that require microbiological protection.
Monomer and Oligomer Transition of Zinc Phthalocyanine Is Key for Photobleaching in Photodynamic Therapy
Authors: Liu Dafeng , Jiang Longguang , Chen Jincan , Chen Zhuo , Yuan Cai , Lin Donghai , Huang Mingdong
Published: June 2023

Photodynamic therapy (PDT) is recognized as a powerful method to inactivate cells. However, the photosensitizer (PS), a key component of PDT, has suffered from undesired photobleaching. Photobleaching reduces reactive oxygen species (ROS) yields, leading to the compromise of and even the loss of the photodynamic effect of the PS. Therefore, much effort has been devoted to minimizing photobleaching in order to ensure that there is no loss of photodynamic efficacy. Here, we report that a type of PS aggregate showed neither photobleaching nor photodynamic action. Upon direct contact with bacteria, the PS aggregate was found to fall apart into PS monomers and thus possessed photodynamic inactivation against bacteria. Interestingly, the disassembly of the bound PS aggregate in the presence of bacteria was intensified by illumination, generating more PS monomers and leading to an enhanced antibacterial photodynamic effect. This demonstrated that on a bacterial surface, the PS aggregate photo-inactivated bacteria via PS monomer during irradiation, where the photodynamic efficiency was retained without photobleaching. Further mechanistic studies showed that PS monomers disrupted bacterial membranes and affected the expression of genes related to cell wall synthesis, bacterial membrane integrity, and oxidative stress. The results obtained here are applicable to other types of PSs in PDT.
Terpinen-4-ol Induces Ferroptosis of Glioma Cells via Downregulating JUN Proto-Oncogene
Authors: Cao Wenpeng , Li Yumei , Zeng Zhirui , Lei Shan
Published: June 2023

According to previous research, turmeric seeds exhibit anti-inflammatory, anti-malignancy, and anti-aging properties due to an abundance of terpinen-4-ol (T4O). Although it is still unclear how T4O works on glioma cells, limited data exist regarding its specific effects. In order to determine whether or not glioma cell lines U251, U87, and LN229 are viable, CCK8 was used as an assay and a colony formation assay was performed using different concentrations of T4O (0, 1, 2, and 4 μM). The effect of T4O on the proliferation of glioma cell line U251 was detected through the subcutaneous implantation of the tumor model. Through high-throughput sequencing, a bioinformatic analysis, and real-time quantitative polymerase chain reactions, we identified the key signaling pathways and targets of T4O. Finally, for the measurement of the cellular ferroptosis levels, we examined the relationship between T4O, ferroptosis, and JUN and the malignant biological properties of glioma cells. T4O significantly inhibited glioma cell growth and colony formation and induced ferroptosis in the glioma cells. T4O inhibited the subcutaneous tumor proliferation of the glioma cells in vivo. T4O suppressed JUN transcription and significantly reduced its expression in the glioma cells. The T4O treatment inhibited GPX4 transcription through JUN. The overexpression of JUN suppressed ferroptosis in the cells rescued through T4O treatment. Taken together, our data suggest that the natural product T4O exerts its anti-cancer effects by inducing JUN/GPX4-dependent ferroptosis and inhibiting cell proliferation, and T4O will hope-fully serve as a prospective compound for glioma treatment.