bluuebunny/crossref_metadata_2025 huggingface dataset:
Tingkat Infeksi Ektoparasit Pada Ikan Gurami (Osprhonemus gouramy) di Pembudidaya Desa Singasari, Kecamatan Karanglewas, Kabupaten Banyumas
Authors: Gayatri Sekar Arum
Published: December 2024

Ikan gurami adalah salah satu jenis ikan yang paling prospek untuk dibudidayakan di Indonesia. Berdasarkan Data Perikanan Indonesia pada tahun 2022 tercatat permintaan akan ikan gurami di Indonesia mencapai 176.113,78 ton atau senilai 6,21 triliun. Serangan parasit adalah salah satu penyakit infeksi yang paling sering menyerang ikan. Organisme yang tergolong parasit diantaranya protozoa, crustacea, dan annelida. Serangan dari parasit ini dapat memicu penurunan kualitas dan pertumbuhan. Tidak hanya itu, produktifitas ikan juga akan menurun, sehingga dapat menimbulkan kerugian secara ekonomi. Penelitian ini bertujuan untuk mengetahui jenis ektoparasit, nilai prevalensi dan intensitas ektoparasit yang menyerang benih gurami. Metode penelitian yang digunakan adalah metode observasi. Sampel yang digunakan adalah benih gurami berukuran 2 - <5 cm dan 5 – 7 cm sebanyak 30 ekor untuk tiap ukuran. Hasil penelitian menunjukan jenis ektoparasit yang ditemukan meliputi Trichodina sp., Chilodonella sp., dan Ichthyoptirius multifiliis. Nilai prevalensi tertinggi mencapai 100% pada ukuran 2 - <5 cm. Sedangkan nilai intensitas tertinggi mencapai 48 ind/ekor pada ukuran 5 – 7 cm.
Penilaian Ekonomi Ekosistem Mangrove di Desa Tritih Kulon dan Karangtalun, Kabupaten Cilacap
Authors: Chairummah Annis , Wisudyanti Dewi , Riviani Riviani , Ekasanti Anandita , Listiowati Emyliana , Nugrayani Dewi , Pelamonia Alamanda
Published: December 2024

Mangrove forests function as a place for the survival of various marine biota such as fish, shrimp, and crabs, especially the mangrove forest area in Tritih Kulon and Karangtalun Villages, Cilacap Regency. Mangrove forests in the area also function as a place for the reproduction of living things that can support the economy of the local community. The purpose of this internship is to determine the economic potential of the mangrove ecosystem and to determine the economic impact of the existence of the mangrove ecosystem on the income of local communities in Tritih Kulon and Karangtalun Villages, Cilacap Regency. This internship was carried out by conducting interviews and distributing questionnaires to obtain data. The results of the internship showed that the economic potential of mangrove forests in the Tritih Kulon area is quite high, namely IDR 330,430,000 and IDR 53,700,000 in Karangtalun Village. The existence of mangrove forests in both locations brings economic value to the local community through tourism activities, fisheries, and home industry production. It is hoped that this research can be a reference in the development of mangrove ecosystems in the future.
Sebaran Kadar pH di Daerah Aliran Sungai Serayu
Authors: Fikriyya Nabela , Firmansyah Faisal Ari , Susanti Susan Tri , Hidayati Nuning Vita
Published: December 2024

Daerah Aliran Sungai (DAS) Serayu merupakan salah satu DAS besar di Jawa Tengah yang menaungi 5 kabupaten, yaitu Kabupaten Wonosobo, Banjarnegara, Purbalingga, Banyumas, dan Cilacap. DAS Serayu menjadi salah satu DAS prioritas yang sekarang ini mengalami kerusakan dan pencemaran lingkungan. Derajat keasaman (pH) merupakan nilai yang menunjukkan konsentrasi ion hidrogen yang masuk kedalam perairan untuk mengetahui keseimbangan asam dan basa. Nilai pH perairan dipengaruhi oleh zat atau bahan asing yang masuk ke badan air, termasuk limbah buangan industri dan rumah tangga. Tujuan penelitian ini yaitu untuk menganalisis sebaran kadar pH dan membandingkan kondisi kadar pH di Daerah Aliran Sungai Serayu dengan baku mutu. Pengambilan sampel dilakukan pada bulan Oktober-November 2023. Pengambilan sampel dilakukan di 15 titik DAS Serayu. Analisis data kualitas air parameter pH menggunakan software ArcGis. Hasil analisis yang didapatkan yaitu nilai pH tertinggi terdapat pada Sungai Serayu Hulu Bendung Singomerto sebesar 7,51 dan nilai pH terendah terdapat pada Sungai Menyawak Bendung Menyawak sebesar 6,18. Data keasaman (pH) Daerah Aliran Sungai Serayu secara umum dari hulu ke hilir menunjukkan trend yang semakin menurun nilai pH nya.
PERLINDUNGAN HUKUM HAK CIPTA TERHADAP DESAIN GRAFIS DALAM MEDIA SOSIAL PINTEREST
Authors: Choirunnisa Farah Tisya , Hadi Hernawan
Published: December 2024

Artikel ini bertujuan untuk mengidentifikasi dan menganalisis mengenai bagaimana perlindungan hukum yang diberikan kepada pemilik hak cipta atas desain grafis dalam media sosial pinterest ditinjau menggunakan Undang-Undang Nomor 28 Tahun 2014 Tentang Hak Cipta. Penelitian ini merupakan penelitian hukum normatif atau doktrinal yang bersifat perspektif dan terapan. Pendekatan yang digunakan adalah pendekatan perundang-undangan dan pendekatan konseptual dengan menggunakan bahan hukum primer dan bahan hukum sekunder. Teknik pengumpulan bahan hukum menggunakan teknik studi dokumen atau studi kepustakaan. Teknis analisis bahan hukum yang digunakan menggunakan metode deduksi dengan silogisme hukum. Hasil peneniltian menunjukkan bahwa Undang-undang Nomor 28 Tahun 2014 Tentang Hak Cipta pada dasarnya telah memberikan perlindungan hukum secara langsung bagi pencipta desain grafis atas desain grafis yang telah diunggahnya dalam media sosial pinterest. Jika terjadi pelanggaran hak cipta atas desain grafis dalam pinterest, pencipta dapat melakukan upaya hukum baik secara preventif maupun represif untuk melindungi ciptaannya.
PROBLEMATIKA PELAKSANAAN RESTRUKTURISASI KREDIT MACET AKIBAT COVID-19 BERDASARKAN PERATURAN OTORITAS JASA KEUANGAN NOMOR 11/POJK.03/2020 (Studi BPR Sami Makmur)
Authors: Yuniar Nirda Okta , Imanullah Moch. Najib
Published: December 2024

This article this thesis law aims to review legal issues to see the implementation of bad credit restructuring due to Covid-19 by BPR Sami Makmur in accordance with the Financial Services Authority Regulation (POJK) Number 11 / POJK.03 / 2020 as well as prohibitions and alternative solutions that can be applied to problems in implementation. bad debt restructuring due to Covid-19 by BPR Sami Makmur in accordance with the Financial Services Authority Regulation (POJK) Number 11 / POJK.03 / 2020.The research method which is empirical legal research is descriptive analytical. The sources of legal materials used are primary and secondary legal materials. The research approach technique used a qualitative approach method. Data observation techniques used document study, interview or observation, observation or observation. The technique of analyzing legal materials uses a syllogistic method that uses a deductive mindset.These results indicate that the implementation of bad debt restructuring is carried out through changes in loan interest rates, additional credit ceilings, extension of credit terms, changes in interest arrears and loan principal, collateral auction or liquidation to simple lawsuit filing. The results of this study also show that during the implementation of the restructuring, including the debtor's lack of education / understanding regarding the restructuring, the debtor does not meet the debt restructuring debtor category, the debtor does not carry out the contents of the credit restructuring according to the agreement.
PROBLEMATIKA PEMENUHAN HAK KONSUMEN DALAM PERATURAN PEMERINTAH NOMOR 80 TAHUN 2019 TENTANG PERDAGANGAN MELALUI SISTEM ELEKTRONIK SERTA PERLINDUNGAN KONSUMEN ATAS POP-UP STORE
Authors: Firmansyah Alfa Edha , . Suraji
Published: December 2024

This article examines the problems of consumer protection against consumer rights with pop-up stores as an alternative to online shopping that can be done in terms of overcoming violations of consumer rights and solutions that can be done to protect consumers against the disadvantages of online shopping. This research is a normative legal research that is descriptive and applied with a legal approach (statue approach), conceptual approach (conceptual approach) and historical approach (historical approach). Types of legal materials include primary and secondary legal materials. The technique of collecting legal materials is a literature study which will then be studied deductively using a syllogistic logic. The results showed the problems of consumer protection for the rights of consumers to shop online and pop-up stores, first, in that consumers cannot directly test, try, see or touch the goods to be ordered when shopping online. Second, the unclear status of legal subjects of business actors. Third, unclear information about the products offered in the form of misleading advertisements. Fourth, the imposition of an unbalanced risk on guaranteed delivery of goods. Fifth, borderless cross-border transactions raise questions about the mechanism for fulfilling consumer rights and obligations of business actors in transactions between countries. Sixth, there are no statutory provisions or implementing provisions for the pop-up store trading model or O2O business (online to offline or offline to online).
Spatiotemporal on Built-Up Area and Population towards Urban Sprawl in Malang City using Google Earth Engine
Authors: Puspita Rafika Tri , Ifan Deffinika  , Purwanto  , Ike Sari Astuti
Published: December 2024

Malang City is the second-most populous city in East Java after Surabaya City. In addition, development in Malang City in recent years has also impacted changes in land use and land cover. The methods used in this research are: 1) built-up area index (EBBI, NDBI, and UI) of Malang City from 2019 to 2023; 2) dasymetric density; 3) distance measurement; and 4) gradient direction. The results of the calculation of the built-up area index show that UI has an average kappa accuracy of 0.75, which is higher than the other two indices. In 2019–2020, the built-up area of Malang City decreased, while in 2021–2023,. There was an increase in the built-up area. This condition is caused by large-scale social restrictions that cause a decrease in development in various sectors. The development of urban sprawl in Malang City in 2019–2023 leads to the north, namely in the districts of Lowokwaru and Blimbing. The correlation test shows that the total population variable has a strong correlation with the urban sprawl (density) variable based on five districts in Malang City.
United center for emergency care and disaster medicine in the Belgorod region: daily work in protracted emergency situations
Authors: Teplov V. M. , Tsebrovskaya E. A. , Karpova E. A. , Grigoriev S. A. , Moskvina S. S. , Bagnenko S. F.
Published: December 2024

Introduction. The trend of uniting the ambulance service in the subject of the Russian Federation has already been introduced in more than half of the regions of our country, but despite this, the arguments in favor of such centralization are still being questioned in the professional community.

The purpose of the study: to study the impact of the association in the Center of Emergency Medical Care and Disaster Medicine on the speciﬁcs of providing emergency medical care to patients in the Belgorod region in working conditions in protracted emergencies.

Materials and methods. A comparative analysis of the activities of the emergency medical service was carried out before and after the creation of the Center for Emergency Medical Care and Disaster Medicine. The obtained results were subjected to statistical processing using the Pearson χ2 test.

Results. According to the results of the study, it was revealed that despite working in conditions of protracted emergencies of a biological, social and military nature, as a result of the merger, it was possible to signiﬁcantly (p<0.05) improve the performance of 20-minute delivery of emergency medical services teams to emergency calls. Thanks to the merger, it was possible to ensure timely evacuation of victims with severe combined injuries to level 1 and 2 trauma centers, as well as patients with suspected acute coronary syndrome to the regional vascular center.

Conclusion. The creation of the Uniﬁed Center for Emergency Medical Care and Disaster Medicine made it possible to organize the eﬀective functioning of the EMS service in the region against the backdrop of a protracted emergency of a biological, social and military nature.
USACE Interference Management Standard v1.0
Authors: Spangler Stephen , Hutsell Steve
Published: December 2024

The Interference Management Standard (IMS) is a comprehensive frame-work designed to streamline the coordination of design, construction, and operation and maintenance models. The IMS provides clear guidelines, defined goals, and objectives to ensure effective interference management. The process encompasses several stages: authoring and compiling models, clash detection, clash analysis, conflict resolution, report compilation, and deliverables submission. By implementing the IMS, users can expect im-proved efficiency and accuracy in model coordination, leading to enhanced project outcomes.
National Creutzfeldt–jakob Disease Research Biobank, a Novel Approach to the Establishment of the Scientific Platform: Collaboration Between Patient Advocacy Group, Scientists, Regulators and Physicians
Authors: Anane Alice , Pasternak Doron , Reisner Shimon A. , Novack Victor
Published: December 2024

Creutzfeldt–Jakob disease (CJD) is a severe neurodegenerative disorder characterized by the abnormal accumulation of prion proteins. In Israel, a unique epidemiological pattern of CJD has been identified, specifically a genetic form (gCJD) associated with the E200K mutation in the PRNP gene. Investigating rare diseases such as CJD syndrome poses challenges due to their low prevalence, hindering the formation of an adequate patient cohort for comprehensive research and treatment trials. To overcome this limitation, biobanks have emerged as transformative tools for collecting and distributing biological specimens along with corresponding health data. Biobanks offer a solution to the inherent heterogeneity in rare diseases, allowing researchers to access diverse and extensive sample sets, thereby enhancing the understanding of disease nuances toward potential therapy. We introduce a novel collaborative model involving the Negev BioBank (NBB), the Creutzfeldt–Jakob Israel Foundation, the Israeli National BioBank for Research (MIDGAM), and the Israeli Ministry of Health. Each entity contributes unique expertise and resources to establish a comprehensive platform for studying the disease. The goal was to establish a participant pool of 500 individuals, including clinically diagnosed cases, confirmed gCJD carriers, and their first- and second-degree relatives. During the pilot phase of the last year, 250 participants were enrolled, with each family contributing between 1 and 25 participants. This collaborative approach involving communities, scientists, physicians, and regulatory bodies establishes a model applicable across various fields. These synergistic efforts aim to advance research on CJD and potentially serve as a blueprint for studying other rare diseases.
Objectively measured moderate-to-vigorous physical activity does not attenuate prospective weight gain among African-origin adults spanning the epidemiological transition.
Authors: Davies Jessica C. , Choo-Kang Candice , Soepnel Larske , Geffen Hayli , Africa Chad , Mtintsilana Asanda , Bovet Pascal , Viswanathan Bharathi , Bedu-Addo Kweku , Boateng Prince Oti , Apusiga Kingsley , Dei Oscar Akunor , Forrester Terrence E. , Williams Marie , Lambert Estelle V. , Rae Dale E. , Sinyanya Nandipha , Layden Brian T. , Gilbert Jack A. , Ecklu-Mensah Gertrude , Joyce Cara , Luke Amy , Dugas Lara R.
Published: December 2024

Traditional obesity-related public health messaging often includes physical activity (PA) recommendations. However, at the population level, the data are conflicting, especially when comparing different self-reported vs measured techniques across different settings and populations. We measured the association between moderate-to-vigorous intensity PA (MVPA) and prospective weight change across five African-origin populations and the extent to which MVPA attenuated weight change over time. At baseline, 2,500 adults (median age: 37y) were recruited into the Modelling the Epidemiologic Transition Study (METS), from Ghana, South Africa, Jamaica, Seychelles, and US. 2000 participants were followed up 8 years later, with 851 participants having complete 7-day accelerometry to measure MVPA at both time points. Generalised estimating equations were used to explore the longitudinal association between weight and MVPA adjusted for several confounders. The obesity prevalence at baseline was 27.5% which increased to 38.0% at follow-up. Baseline MVPA varied from 7 (IQR: 4, 16) min/day in US women to 52 (IQR: 36, 78) min/day in South African men, and similarly at follow-up ranged from 8 min/day to 41 min/day among the same participant groups. While overall, engaging in higher MVPA levels was associated with a lower body weight, such that every additional 30 min of MVPA equalled a 600g lower body weight (p = 0.04), the interaction between time and MVPA was not statistically significant (p = 0.18). Therefore, regardless of the amount of MVPA at any time point, body weight increased over time. Despite the association between MVPA and weight, our results suggest that objectively measured longitudinal MVPA was not associated with the change in 8-year weight in African-origin adults. Our research confirms that while PA is a critical determinant of cardiovascular health, it alone may not be enough to stem the rising obesity burden.
NEIL3 Promotes the Carcinogenesis of Prostate Cancer by Activating PI3K/Akt/mTOR Signaling
Authors: Zhang Wei , Liu Zihao , Wen Simeng , Shao Yuan , Yang Zhen , Wang Yong
Published: December 2024

Prostate cancer (PCa) is one of the most common cancers worldwide. Nei endonuclease VIII-like 3 (NEIL3) plays important roles in diverse cancers. In this study, we found that NEIL3 was over-expressed in PCa tissues and cell lines. NEIL3 over-expression is associated with worse prognostic outcomes in PCa patients. In vitro, PCa cell proliferation, invasion, and migration could be significantly inhibited with knocking down NEIL3 by inactivating the phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of the rapamycin (mTOR) signaling. Besides, based on the results of RNA-sequencing, we found that the protein expression of high-mobility gene group A2 and androgen receptor (AR) were decreased in C4-2 cells treated with siNEIL3. Therefore, this result suggests that NEIL3 regulates the progression of PCa by modulating the PI3K/AKT/mTOR signaling and the expression of AR.
Effect of Different Doses of Ciprofol on Hemodynamics Induced By General Anesthesia in Elderly Diabetic Patients Undergoing Spinal Surgery: A double-blind, randomized, controlled study
Authors: Jiang Xiao-rui , Zhang Lin-zhong , Ji Jia-wei , Jing Yan , Li Mei-ping
Published: December 2024

Objective To investigate the safety and efficacy of different doses ciprofol in induction of general anesthesia in elderly diabetic patients.
Methods Ninety spinal single-level PLIF patients who were planned to undergo elective tracheal intubation under general anesthesia were selected as the subjects.The study subjects were randomly divided into 3 groups. Ciprofol induction dose was 0.2 mg/kg in group A, 0.3 mg/kg in group B and 0.4 mg/kg in group C, The safety and efficacy of general anesthesia induction were compared among the three groups.
Results The incidence of hypotension in group C (46.4%) was significantly higher than that in group A (7.4%) and group B (14.8%) (P < 0.05). AUCMAP of the three groups was higher in group C (176.39±33.83 mmHg.min) than in group B (158.44±26.55 mmHg.min) and higher in group B than in group A (143.59±19.52 mmHg.min), P < 0.05. The incidence of intubation reaction in group A (77.8%) was the highest (P < 0.05). There were significant differences in BIS values at 3, 4 and 5 minutes after administration.
Conclusion The use of 0.3mg/kg ciprofol in the induction period in elderly diabetic patients has more stable circulation and less vasoactive drug dosage during induction, which is better than 0.2 and 0.4mg/kg dosage groups. The trial was registered at Chinese ClinicalTrials.gov in February 26, 2024 (Registration number:ChiCTR2400081164).
An Analytical Approach for Determining Macro- and Micro- Elements in Two Different Growing Periods of Artemisia abrotanum L
Authors: Cansever İslam , Sogut Ozlem
Published: December 2024

The widespread use of medicinal plants has led to the cultivation of Artemisia abrotanum L., which grows wild in Turkey. The amount of trace elements contained in plants is important for health therefore,analyses of microwave and infusion extracts of the plant were performed using ICP-MS. The evaluation of the elemental content of the harvest time of Artemisia abrotanum L. using a validated method constitutes the aim of the study. Na, K, Mg, Fe, P, S, Ca, Al, V, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Sr, Mo, Cd, Ba, Tl, Li, U were determined. It was observed that the element distribution varied depending on the element in the pre-flowering and flowering periods of the plant. In both extraction methods, Na, Mn, Ni, Co, As, Se, Sr, and Ba concentrations were higher in the blooming period and K, Mg, Ca, Zn, Mo, Cd, and Pb concentrations were higher in the pre-blooming period of Artemisia abratonum L. The amounts of P, Fe, Al, V, Cr, and Tl concentrations differed according to the extraction method. Cu and S concentrations were determined to be similar in both extraction methods and at both harvest times. The results were classified by chemometric analysis and the preparation method and elemental relationships were evaluated. When evaluating the amounts of essential, beneficial, and harmful elements present in Artemisia abrotanum L., consumed as an infusion by the public, independent of harvest time, we found that the detected levels of these elements exceeded the required or harmful thresholds for human health.
Clinical Correlation of CD39 Expression on Killer T Cells in peripheral blood in Cervical Squamous Cell Carcinoma
Authors: LI MENG , Jin Hao , Han Dongmei
Published: December 2024

Aim
To study the correlation between CD39 expression level of CD3 + CD8 + T cells in peripheral blood of patients with cervical squamous cell carcinoma (CESC) and the clinical information.
Methods
This study enrolled 80 participants with CESC, their peripheral blood samples were collected, and flow cytometry was used to detect and analyze the number of CD3 + CD8 + T cells and the expression levels of CD39 and cytokines (IFN-γ and TNF-α) in peripheral blood. Clinical information of tumor patients was collected, and the correlation between the expression levels and clinical characteristics was analyzed and preliminary mechanism exploration was carried out.
Results
The study found that the expression level of CD39 molecule on the surface of CD3 + CD8 + T cells in the peripheral blood of patients with CESC was correlated with the patients' FIGO stage, lymph node metastasis and distant metastasis; IFN-γand TNF-α secretion levels were correlated. This study found that the proportion of CD8 + CD39 + T cells in patients without lymph node and distant metastases was higher than that in patients with lymph node and distant metastases (P < 0.05). This study also found that CESC patients with a higher percentage of CD8 + CD39 + T cells in CD3 + T cells could secrete more IFN-γand TNF-α (P < 0.0001).
Conclusion
In this study, bioinformatics analysis of CESC patients and the detection of the expression level of CD39 molecule on the surface of CD3 + CD8 + T cells in peripheral blood showed that the expression level of CD39 molecule in CESC is related to disease progression. Further studies have found that CD8 + CD39 + T cell subsets have anti-tumor properties and better prognosis for patients.
Effect of Music Therapy on Emotional Resilience, Well-Being, and Employability: A Quantitative Investigation of Mediation and Moderation
Authors: Yingjie Feng , Mingda Wang
Published: December 2024

This study explores the impact of music therapy on emotional resilience, well-being, and employability. Through an 8-week music therapy intervention involving 256 participants, the results demonstrated that music therapy significantly enhanced participants' emotional resilience, which in turn improved their well-being and employability. A significant positive correlation was found between emotional resilience, well-being, and employability, with well-being mediating the relationship between emotional resilience and employability. Additionally, the study identified moderating effects of age and education level, revealing that younger individuals and those with higher education levels benefit more from emotional resilience in terms of well-being and employability. These findings suggest that music therapy is an effective intervention for enhancing emotional resilience and career development, with well-being playing a crucial role in this process. Future research should continue to investigate the long-term benefits of music therapy, explore its effects across diverse populations, and incorporate objective measures and longitudinal designs to validate and expand upon these findings.
Leadless pacemaker implantation after delayed atrial lead perforation and pacemaker energy exhaustion: A case report
Authors: Zhao Yichang , Su Liping , Gao Yuchen , Wang Hao , Luan Chao , Liu Jinqiu , Chen Feifei
Published: December 2024

Background
Delayed lead perforation is a rare complication of a cardiac implantable electronic device (CIED). Clinical presentations range from completely asymptomatic to pericardial tamponade. Surgical lead extraction is recommended and percutaneous lead extraction with surgical backup is an alternative method.
Case presentation
A male with a history of paroxysmal atrial fibrillation and sick sinus syndrome implanted a dual-chamber pacemaker with two passive fixation lead. He was on oral anticoagulants and played golf for almost 1 hour every day after implantation. However, he complained of thoracic stabbing in the sternal manubrium with abnormal findings on pacemaker interrogation. Imaging confirmed the perforated atrial electrode with lead tip protrusion from the pericardium adjacent to the inferior wall of the main right pulmonary artery, but with no pericardial effusion. A lead removal by transvenous approach with surgical support was suggested, but the patient refused. Given the stable condition of the patient, conservative treatment was chosen in the absence of complications during a follow-up period of 14 years. Then pacemaker energy was exhausted with a ventricular lead threshold increased. A leadless pacemaker was implanted.
Conclusions
Chest pain in CIED with abnormal electrical parameters, especially ongoing treatment with anticoagulants and regular physical activity, should always raise suspicion of lead perforation. A conservative strategy may be appropriate and feasible for those in the absence of perforation-related complications. For patients with pacemaker energy exhaustion combined with ventricular lead aging after conservative treatment of lead perforation, leadless pacemaker may be an alternative approach.
Plasma cfDNA VILL gene methylation as a diagnostic marker for nasopharyngeal carcinoma
Authors: Fu Xiao-Yan , Zhou Zi-Ying , Yang Teng-Yue , Wen Ying-Juan , Liu Da-Bo , Zhou Yi-Bo , Yuan Yue , Ye Fei , Huang Zhong-Xi
Published: December 2024

Currently, the non-invasive diagnostic methods for nasopharyngeal carcinoma (NPC) continue to grapple with the challenge of low sensitivity. The hypermethylation of tumor suppressor genes is an established early event in NPC pathogenesis. Consequently, we conducted whole-genome methylation sequencing on plasma cell-free DNA (cfDNA) from six NPC cases and four healthy controls, integrating Illumina Human Methylation 450K microarray data from the GEO database comprising six NPC cases and six samples of non-cancerous nasopharyngeal tissue (NP). As result, we screened only one CpG island associated with cell type-specific regulation within the tumor suppressor gene VILL, which exhibits specific methylation patterns in NPC. We validated our findings using 25 pairs of NPC and NP samples from GEO, alongside 9,736 pan-cancer tissues from TCGA and 656 healthy human leukocyte samples sourced from GEO through methylation microarray analysis. Based on this, we designed a methylation-specific qPCR (qMSP) system for the VILL gene, and then tested it on 192 primary NPC and 154 NC plasma samples. The new qMSP system when compared with EBV DNA qPCR revealed a sensitivity for primary NPC of 80.2% vs.81.3% (78.8% vs.54.5% for early-stage NPC), and a specificity of 100% vs. 93.5%. Notably, employing a combined methodology further enhanced sensitivity to 94.8%, including a sensitivity rate of 90.9% for early-stage NPC diagnosis. Therefore, VILL methylation assessment combined with EBV DNA detection presents a promising avenue for non-invasive diagnosis of NPC, particularly beneficial for early detection.
Does Gender Matter? A Qualitative Analysis of Teaching Award Nominations of General Surgery Chief Residents By Medical Students
Authors: McDermott Colleen E , Stonehocker Dellene , Sun Ting , Lambert Laura
Published: December 2024

Purpose
Gender disparities have been identified in surgical resident teaching award distribution. Qualitative analysis of resident and faculty teaching award nominations has been performed previously; however, qualitative analysis of students' nominations of residents for teaching awards by gender has not yet been explored.
Methods
Nominations for resident teaching awards from the past four years at a single institution were grouped by resident gender. Nominations were then analyzed for thematic content via inductive coding methods using MAXQDA software.
Results
All residents identified as male or female. 40 nominations (13 male, 17 female) were analyzed. The following themes emerged: 1 )Inclusivity 2) Commitment to Teaching 3) Teaching Efficacy 4)Approval of Resident/Role Modeling. Subcategories of major themes were also identified. Many categories were similar for all residents. Notable areas that differed by > 5% of coded text included: female residents were more likely to be described as kind or approachable, and were more commonly described as teaching a specific skill. Male residents were more likely to be described as incorporating the med student into the team, and were more commonly mentioned for teaching in the OR.
Conclusion
This analysis of medical student nominations for resident teaching awards shows areas of thematic difference between male and female surgical residents and provides insight into how learners may assess teaching skills differently by gender. Further study into how this may apply to faculty and impact the learning environment is warranted.
mBAT: A Newly Developed Mobile Application for Self-Screening of Pediatric Bleeding Disorders – A Multi-Center Study
Authors: Sermcheep Arpatsorn , Natesirinilkul Rungrote , Komvilaisak Patcharee , Songthawee Natsaruth , Laohverapanich Kamala , Songdej Duantida , Wongwerawattanakoon Pakawan , Kadegasem Praguywan , Chuansumrit Ampaiwan , Sirachainan Nongnuch
Published: December 2024

Background
Bleeding assessment tools (BATs) are used by trained medical personnel for screening bleeding disorders on a one-to-one basis with patients; hence, they are time consuming and limited its use for large population screening
Objectives
To develop, validate, and demonstrate a Thai version mobile application (mBAT) for self-screening of bleeding disorders
Methods
mBAT was developed and validated from the paper-based Thai pediatric bleeding questionnaire (TPBQ). Then, mBAT was applied to 916 subjects from communities and hematology clinics. Those with scores ≥ 3 were consented for further investigation of bleeding disorders.
Results
mBAT was developed for Thai children using simple sentences, video, pictures, and auto-calculation. It showed a high correlation with the TPBQ: r = 0.96 and 0.97 in the PBQ and ISTH scoring systems, respectively (P < 0.01). A score ≥ 3 was considered abnormal. The mBAT was applied to 916 subjects, with a mean (range) age of 10.5 (0.07-18.0) years. The majority of the subjects (97.3%) were from the community; and the rest (2.7%) were from hematology clinics. The results showed 41 subjects had abnormal scores, and 35 of them (16 from the community and 19 from clinics) consented for diagnostic investigation. Eleven subjects (31.4%) were found to have bleeding disorders, including four with VWD, two each with hemophilia A and thrombocytopenia, and one each with factor VII deficiency, platelet dysfunction, and MYH9-related disorder. Thus, the prevalence rates of bleeding disorders in the subjects whose score ≥ 3 from community and hematology clinics were 6.25% and 52.6%, respectively.
Conclusion
The mBAT had a high correlation with the TPBQ. As a self-screening tool, it could enhance the diagnosis of bleeding disorders.
ANKRD22 Participates in Pro-inflammatory Activities of Macrophages in the Tumor Microenvironment of Colon Cancer
Authors: Wang Xiaoying , Yang Keqing , Yang Bin , Wang Rui , zhu yongliang , Pan Tianhui
Published: December 2024

Tumor-associated macrophages (TAMs) are among the most common types of immune cells in the colon cancer microenvironment. Reprogramming M2-type TAMs with immunosuppressive functions into M1-type TAMs with pro-inflammatory functions is a novel strategy for reshaping the tumor microenvironment (TME) and enhancing the efficacy of immunotherapy in colon cancer. However, the key molecules and mechanisms underlying TAM polarization require further clarification. Our previous study suggested that ANKRD22 may play a role in regulating the functional state transition of macrophages. However, the expression levels of ANKRD22 in colon TAMs and its specific effects on tumor proliferation remain unclear. In the current study, we observed elevated ANKRD22 expression in M1-type TAMs. The expression level of ANKRD22 correlated positively with the survival period of patients with colon cancer, as well as with the infiltration abundance of M1-type TAMs and negatively with the infiltration abundance of M2-type TAMs. A significant decrease in ANKRD22 expression in macrophages co-cultured with colon cancer cell culture supernatant as well as in macrophages directly derived from colorectal cancer tissues were observed. Single-cell RNA sequencing, spatial transcriptomic studies, and subcutaneous xenograft experiments in mice showed that silencing of Ankrd22 altered the subtype distribution of macrophages, attenuated their pro-inflammatory activity, and enhanced their pro-tumor activity. Finally, we identified a potential ANKRD22 agonistic small-molecule lead compound that could contribute to the development of novel therapeutics based on TAM remodeling.
An effective heuristic for developing hybrid feature selection in high dimensional and low sample size datasets
Authors: Shin Hyunseok , Oh Sejong
Published: December 2024

Background.
High-dimensional datasets with low sample sizes (HDLSS) are pivotal in the fields of biology and bioinformatics. One of core objective of HDLSS is to select most informative features and discarding redundant or irrelevant features. This is particularly crucial in bioinformatics, where accurate feature (gene) selection can lead to breakthroughs in drug development and provide insights into disease diagnostics. Despite its importance, identifying optimal features is still a significant challenge in HDLSS.
Results.
To address this challenge, we propose an effective feature selection method that combines gradual permutation filtering with a heuristic tribrid search strategy, specifically tailored for HDLSS contexts. The proposed method considers inter-feature interactions and leverages feature rankings during the search process. In addition, a new performance metric for the HDLSS that evaluates both the number and quality of selected features is suggested. Through the comparison of the benchmark dataset with existing methods, the proposed method reduced the average number of selected features from 37.8 to 5.5 and improved the performance of the prediction model, based on the selected features, from 0.855 to 0.927.
Conclusions.
The proposed method effectively selects a small number of important features and achieves high prediction performance.
Bedtime snacking deteriorates continuous glucose monitoring metrics in young children with type 1 diabetes: a randomized controlled crossover trial
Authors: Gökçe Tuğba , Karakuş Kağan , Mutlu Gül Yeşiltepe , Muradoğlu Serra , Eviz Elif , Can Ecem , Smart Carmel E , Hatun Şükrü , Özel Hülya Gökmen
Published: December 2024

Objective
To determine if a bedtime snack in young children with type 1 diabetes (T1D) prevents nocturnal hypoglycemia, and the impact on glycemia overnight.
Methods
In this randomized controlled crossover trial, 10 grams of carbohydrate (milk, yogurt, and kefir) was given 150–180 minutes after dinner over three nights to 5-8-year-old children with T1D using multiple daily injection therapy. Continuous glucose monitoring (CGM) data were collected for 6 hours following the snacks on one control and three snack nights. Time in 70–180 mg/dl range (TIR), time below 70 mg/dL (TBR), and other metrics were analyzed according to international CGM consensus. Trial day was terminated if blood glucose exceeded 300 mg/dL or fell below 70 mg/dL.
Results
Of 28 children (13 female, mean age 6.6 ± 0.8 years, HbA1c 7.0 ± 0.5%), mean glucose values before the test snacks were 137.8 ± 14.5 mg/dL for milk, 141.9 ± 16.9 mg/dL for yogurt, 136 ± 19.1 mg/dL for kefir, and 140.8 ± 17.0 mg/dL for control without significant difference (p = 0.548). TIR was 34.7% for milk, 38.7%. for yogurt, 45.9% for kefir, and 75.5% for control during the 6-hour post snack period, with TIR on the control day significantly higher than the three snack days (p < 0.001). TBR did not differ by group (p > 0.05). Of 112 trial days, 13 days were terminated due to hyperglycemia (> 300 mg/dL) (8 milk, 4 yogurt, 1 kefir), and 3 trial days due to hypoglycemia (< 70 mg/dL) (1 yogurt, 2 control).
Conclusion
Bedtime snacking in young children with T1D impairs nocturnal glycemia and reduces TIR, without decreasing the frequency of hypoglycemia.
Optimizing clinical nursing pathways to reduce the delay of thrombolysis in patients with acute ischemic stroke
Authors: Jiao Feitong , Wang Chunyan , Lu Jing , Zhou Pingping
Published: December 2024

Objective
To evaluate the effectiveness of optimizing clinical nursing pathways in reducing the delay of thrombolysis in patients with acute ischemic stroke.
Methodology: Process optimization implementation from July 2021 to December 2021 AIS patients who came to the emergency department of the Affiliated Hospital of Jilin Medical University (Jilin China) were included in the control group, referred to as T1. Process optimization from July 2022 to December 2022 The AIS patients who visited the clinic after implementation belonged to the T2 group. In June 2022, it will be the trial operation stage of optimizing the treatment process in the hospital, and will not be included in the study. The time from hospital admission to thrombolytic treatment and the situation of thrombolysis in the two groups were collected, including the time of physical assessment of the patients, CT time, the time of signing the thrombolysis agreement by the family members of the patients, and the time when the doctor was in place. Group T2 has stroke specialist nurses who participated in in-hospital training to guide the process and participate in the whole process of treatment.
Results
The median DNT in the T2 group was shortened from 39.5 minutes in the T1 group to 28.5 minutes, the proportion of DNT ≤ 45min and DNT ≤ 30min increased from 77.2–88.8%, and from 21.1–67.5%, the differences were statistically significant (all P < 0.01); the median on-site time of clinic doctors and thrombolytic doctors was shortened from 2.9 minutes to 2.6 minutes, and from 5.1 minutes to 4.3 minutes, respectively, with statistically significant differences (all P < 0.0001); process links The median time for the physical assessment of the middle-aged patients and the time from the completion of CT to the signing of the informed consent for intravenous thrombolysis were shortened from 15.2 minutes to 8.5 minutes, and from 11.6 minutes to 6.2 minutes (all P < 0.0001); transfer the patient to the CT and call the clinic The median time from the doctor to the completion of the physical examination and from the signing of the informed consent to the start of thrombolysis was shortened from 3.0 minutes to 2.6 minutes, 6.8 minutes to 6.7 minutes, and 4.3 minutes to 4.0 minutes respectively (all P < 0.05); There was no significant difference in NIHSS scores before treatment, 24 hours after treatment, and one week after treatment (P > 0.05). After 3 months of treatment, the proportion of mRS ≤ 2 points increased, but the differences in indicators were not statistically significant (P > 0.05).
Conclusion
The optimized clinical nursing path reduces the delay of thrombolysis in patients with acute ischemic stroke.
Influence of Sex and Gender on Knowledge of Generic Medicines Among Older Patients and Caregivers
Authors: Ceolin Chiara , Salis Francesco , Macaluso Myriam , Levati Elena , Zazzara Maria Beatrice , Onder Graziano , Bernabei Roberto , Trevisan Caterina , D'Ignazio Federica , Rivasi Gilia
Published: December 2024

Purpose
Generic medicines (GMs) are bioequivalent to brand-name drugs and more cost-effective, yet their use is limited. Gender-related factors may influence attitudes towards GMs, but data on older adults are scarce. This study aimed to explore sex and gender differences in knowledge and opinions about GMs among older patients and their caregivers.
Methods
SurFE is a cross-sectional multicenter survey conducted in Italy (April-May 2023) that enrolled non-institutionalized individuals aged ≥ 65 years and their caregivers. Participants completed a questionnaire assessing knowledge, attitudes, and perceptions of GMs, and sociodemographic and health-related information. We collected data on gender-specific factors and, using a validated methodology, developed a composite gender score.
Results
Among the 471 participants (312 patients and 159 caregivers), no differences related to biological sex were observed. According to the gender score, patients with male gender characteristics were less likely to believe that generic medicines are made with lower-quality substances. Additionally, both female patients and male caregivers were more likely to consider GMs equivalent to brand-name medications. Logistic regression analysis showed that female caregivers were significantly more likely to believe that GMs are produced with lower-quality substances (OR = 2.06, 95% CI 1.01–4.21, p = 0.047). No other significant associations between sex, gender, and beliefs about GMs were found.
Conclusions
Gender-related factors may have a greater impact on shaping beliefs about GMs than biological sex. Further research is required to explore the potential role of other sociodemographic or clinical factors to better understand and address barriers to the use of GMs.
Testing of a reusable chemical warming pad and an insulating jacket to manage hypothermia of preterm and low birthweight neonates
Authors: Ahmed Anisuddin , Rahman Fariya , Islam Md. Mahinur , Patwary Hridoy , Tanvir K M , Ahmed Saifuddin , Hussam Abul , Islam Mominul , Målqvist Mats , Rahman Ahmed Ehsanur , Arifeen Shams El , Rahman Syed Moshfiqur
Published: December 2024

Hypothermia remains a leading contributing factor to neonatal mortality. This study reports testing of a thermoregulatory device— ‘Thermal Jacket’ that includes a reusable chemical warming pad(CWP) and an insulating jacket designed for hypothermia management. The laboratory experiments were conducted in two distinct phases between February’21 and June’22. In phase 1, a ternary composite of Sodium-Acetate-Trihydrate, Glycerol, Paraffin, and water contained in a high-density polyethylene-pouch named ‘CWP’ was finalised, and an insulating jacket was designed for targeted heat retention. In phase 2, the device’s efficacy was evaluated using mannequin in a controlled setting. The sample size was 81 events. Welch's t-test, ANOVA, and GEE were used to assess any significant differences in performance with success. Among 81 events, approximately 93% of events of CWP and 98% of events of insulating jacket successfully maintained temperatures within 36–38°C for 120 minutes. Moreover, neither ambient temperature nor repeated use of CWPs had any significant effect on success rate of CWP and insulating jacket. Thermal Jacket had achieved and sustained the temperature range of 36–38°C for 2 hours. While this study used mannequin, rigorous systematic testing with preterm or low birthweight neonates in clinical settings is imperative to assess its effectiveness for thermal care management.
Increased Detection of Desulfobacterota in Wastewater Treatment Plant Effluent via Composite Sampling Versus Grab Sampling
Authors: Jurvansuu Jaana , Länsivaara Annika , Palmroth Marja , Kaarela Outi , Hyöty Heikki , Oikarinen Sami , Lehto Kirsi-Maarit
Published: December 2024

Municipal wastewater surveillance is essential for public health monitoring and preventing environmental contamination. Analysing treatment plant influents offers insights into population exposure to chemicals and microbes, while effluent and activated sludge reflect treatment efficiency and the quality of substances released into the environment. Our study, which compared grab and 24-hour composite bacterial sampling methods using next-generation sequencing of the 16S V4 region, offers practical insights. We collected samples from influent, activated sludge from the activated sludge process, and effluent wastewater on eight dates in 2017-2018 at wastewater treatment plants in Pirkanmaa, Finland. The results showed distinct bacterial compositions across sample types, with composite effluent samples revealing significantly higher Desulfobacterota abundance than the grab sample, thereby highlighting its higher resolution for effluent sampling.