bluuebunny/crossref_metadata_2025 huggingface dataset:
Least-Squares Estimators of Drift Parameter for Discretely Observed Fractional Ornstein–Uhlenbeck Processes
Authors: Kříž Pavel , Szała Leszek
Published: May 2020

We introduce three new estimators of the drift parameter of a fractional Ornstein–Uhlenbeck process. These estimators are based on modifications of the least-squares procedure utilizing the explicit formula for the process and covariance structure of a fractional Brownian motion. We demonstrate their advantageous properties in the setting of discrete-time observations with fixed mesh size, where they outperform the existing estimators. Numerical experiments by Monte Carlo simulations are conducted to confirm and illustrate theoretical findings. New estimation techniques can improve calibration of models in the form of linear stochastic differential equations driven by a fractional Brownian motion, which are used in diverse fields such as biology, neuroscience, finance and many others.
Are Helicobacter pylori Infection and Fucoidan Consumption Associated with Fucoidan Absorption?
Authors: Tomori Makoto , Nagamine Takeaki , Iha Masahiko
Published: May 2020

We examined the associations of Helicobacter pylori and mozuku consumption with fucoidan absorption. Overall, 259 Japanese volunteers consumed 3 g fucoidan, and their urine samples were collected to measure fucoidan values and H. pylori titers before and 3, 6, and 9 h after fucoidan ingestion. Compared to the basal levels (3.7 ± 3.4 ng/mL), the urinary fucoidan values significantly increased 3, 6, and 9 h (15.3 ± 18.8, 24.4 ± 35.1, and 24.2 ± 35.2 ng/mL, respectively) after fucoidan ingestion. The basal fucoidan levels were significantly lower in H. pylori-negative subjects who rarely ate mozuku than in those who regularly consumed it. Regarding the ΔMax fucoidan value (highest value − basal value) in H. pylori-positive subjects who ate mozuku at least once a month, those aged ≥40 years exhibited significantly lower values than <40 years old. Among subjects ≥40 years old who regularly consumed mozuku, the ΔMax fucoidan value was significantly lower in H. pylori-positive subjects than in H. pylori-negative ones. In H. pylori-positive subjects who ate mozuku at least once monthly, basal fucoidan values displayed positive correlations with H. pylori titers and ΔMax fucoidan values in subjects <40 years old. No correlations were found in H. pylori-positive subjects who ate mozuku once every 2–3 months or less. Thus, fucoidan absorption is associated with H. pylori infection and frequency of mozuku consumption.
Identification, Cloning and Heterologous Expression of the Gene Cluster Directing RES-701-3, -4 Lasso Peptides Biosynthesis from a Marine Streptomyces Strain
Authors: Oves-Costales Daniel , Sánchez-Hidalgo Marina , Martín Jesús , Genilloud Olga
Published: May 2020

RES-701-3 and RES-701-4 are two class II lasso peptides originally identified in the fermentation broth of Streptomyces sp. RE-896, which have been described as selective endothelin type B receptor antagonists. These two lasso peptides only differ in the identity of the C-terminal residue (tryptophan in RES-701-3, 7-hydroxy-tryptophan in RES-701-4), thus raising an intriguing question about the mechanism behind the modification of the tryptophan residue. In this study, we describe the identification of their biosynthetic gene cluster through the genome mining of the marine actinomycete Streptomyces caniferus CA-271066, its cloning and heterologous expression, and show that the seven open reading frames (ORFs) encoded within the gene cluster are sufficient for the biosynthesis of both lasso peptides. We propose that ResE, a protein lacking known putatively conserved domains, is likely to play a key role in the post-translational modification of the C-terminal tryptophan of RES-701-3 that affords RES-701-4. A BLASTP search with the ResE amino acid sequence shows the presence of homologues of this protein in the genomes of eight other Streptomyces strains, which also harbour the genes encoding the RES-701-3, -4 precursor peptide, split-B proteins and ATP-dependent lactam synthetase required for the biosynthesis of these compounds.
New Cytotoxic Natural Products from the Red Sea Sponge Stylissa carteri
Authors: Abdelhameed Reda F. A. , Habib Eman S. , Eltahawy Nermeen A. , Hassanean Hashim A. , Ibrahim Amany K. , Mohammed Anber F. , Fayez Shaimaa , Hayallah Alaa M. , Yamada Koji , Behery Fathy A. , Al-Sanea Mohammad M. , Alzarea Sami I. , Bringmann Gerhard , Ahmed Safwat A. , Abdelmohsen Usama Ramadan
Published: May 2020

Bioactivity-guided isolation supported by LC-HRESIMS metabolic profiling led to the isolation of two new compounds, a ceramide, stylissamide A (1), and a cerebroside, stylissoside A (2), from the methanol extract of the Red Sea sponge Stylissa carteri. Structure elucidation was achieved using spectroscopic techniques, including 1D and 2D NMR and HRMS. The bioactive extract’s metabolomic profiling showed the existence of various secondary metabolites, mainly oleanane-type saponins, phenolic diterpenes, and lupane triterpenes. The in vitro cytotoxic activity of the isolated compounds was tested against two human cancer cell lines, MCF-7 and HepG2. Both compounds, 1 and 2, displayed strong cytotoxicity against the MCF-7 cell line, with IC50 values at 21.1 ± 0.17 µM and 27.5 ± 0.18 µM, respectively. They likewise showed a promising activity against HepG2 with IC50 at 36.8 ± 0.16 µM for 1 and IC50 30.5 ± 0.23 µM for 2 compared to the standard drug cisplatin. Molecular docking experiments showed that 1 and 2 displayed high affinity to the SET protein and to inhibitor 2 of protein phosphatase 2A (I2PP2A), which could be a possible mechanism for their cytotoxic activity. This paper spreads light on the role of these metabolites in holding fouling organisms away from the outer surface of the sponge, and the potential use of these defensive molecules in the production of novel anticancer agents.
Separation of Zn(II), Cr(III), and Ni(II) Ions Using the Polymer Inclusion Membranes Containing Acetylacetone Derivative as the Carrier
Authors: Radzyminska-Lenarcik Elzbieta , Pyszka Ilona , Ulewicz Malgorzata
Published: May 2020

Polymer inclusion membranes (PIMs) doped with ethylenodiamino-bis-acetylacetone as fixed carrier was applied for the investigation of the facilitated transport of Zn(II), Cr(III), and Ni(II) ions from an aqueous nitrate feed phase (cM = 0.001 mol/dm3). The optimal membrane composition (amount of carrier and o-NPPE-plasticizer) was determined. For the optimal polymer inclusion membranes doped with ethylenodiamino-bis-acetylacetone, the following patterns of transport selectivity were found: Zn(II) > Cr(III) > Ni(II). The initial flux of Zn(II), Cr(III), and Ni(II) ions was 6.37 µmol/m2∙s, 5.53 µmol/m2∙s, and 0.40 µmol/m2∙s, respectively. The selectivity coefficients equal to 1.2 and 15.9 were found for Zn(II)/Cr(III) and Zn(II)/Ni(II), respectively. After 24-h transport, the recovery factor of Zn(II), Cr(III), and Ni(II) were 90%, 65%, and 6%, respectively. The polymer inclusion membranes doped with ethylenodiamino-bis-acetylacetone were characterized by scanning electron microscopy and non-contact atomic force microscopy. The influence of membrane morphology on transport process was discussed.
Effects of Testing Temperature on the Serration Behavior of an Al–Zn–Mg–Cu Alloy with Natural and Artificial Aging in Sharp Indentation
Authors: Yamada Hiroyuki , Kami Tsuyoshi , Ogasawara Nagahisa
Published: May 2020

Serration phenomena, in which stress fluctuates in a saw-tooth shape, occur when a uniaxial test is performed on an aluminum alloy containing a solid solution of solute atoms. The appearance of the serrations is affected by the strain rate and temperature. Indentation tests enable the evaluation of a wide range of strain rates in a single test and are a convenient test method for evaluating serration phenomena. Previously, the serrations caused by indentation at room temperature were clarified using strain rate as an index. In this study, we considered ambient temperature as another possible influential factor. We clarify, through experimentation, the effect of temperature on the serration phenomenon caused by indentation. An Al–Zn–Mg–Cu alloy (7075 aluminum alloy) was used as the specimen. The aging phenomenon was controlled by varying the testing temperature of the solution-treated specimen. Furthermore, the material properties obtained by indentation were evaluated. By varying the testing temperature, the presence and amount of precipitation were controlled and the number of solute atoms was varied. Additionally, the diffusion of solute atoms was controlled by maintaining the displacement during indentations, and a favorable environment for the occurrence of serrations was induced. The obtained results reveal that the variations in the serrations formed in the loading curvature obtained via indentation are attributed to the extent of interaction between the solute atoms and the dislocations.
Development and Characterization of a Novel Low-Cost Water-Level and Water Quality Monitoring Sensor by Using Enhanced Screen Printing Technology with PEDOT:PSS
Authors: Wang Bei , Baeuscher Manuel , Hu Xiaodong , Woehrmann Markus , Becker Katharina , Juergensen Nils , Hubl Moritz , Mackowiak Piotr , Schneider-Ramelow Martin , Lang Klaus-Dieter , Ngo Ha-Duong
Published: May 2020

A novel capacitive sensor for measuring the water-level and monitoring the water quality has been developed in this work by using an enhanced screen printing technology. A commonly used environment-friendly conductive polymer poly(3,4-ethylenedioxythiophene):poly (styrenesulfonate) (PEDOT:PSS) for conductive sensors has a limited conductivity due to its high sheet resistance. A physical treatment performed during the printing process has reduced the sheet resistance of printed PEDOT:PSS on polyethylenterephthalat (PET) substrate from 264.39 Ω/sq to 23.44 Ω/sq. The adhesion bonding force between printed PEDOT:PSS and the substrate PET is increased by using chemical treatment and tested using a newly designed adhesive peeling force test. Using the economical conductive ink PEDOT:PSS with this new physical treatment, our capacitive sensors are cost-efficient and have a sensitivity of up to 1.25 pF/mm.
Microbial Metabolic Potential of Phenol Degradation in Wastewater Treatment Plant of Crude Oil Refinery: Analysis of Metagenomes and Characterization of Isolates
Authors: Viggor Signe , Jõesaar Merike , Soares-Castro Pedro , Ilmjärv Tanel , Santos Pedro M. , Kapley Atya , Kivisaar Maia
Published: May 2020

The drilling, processing and transportation of oil are the main sources of pollution in water and soil. The current work analyzes the microbial diversity and aromatic compounds degradation potential in the metagenomes of communities in the wastewater treatment plant (WWTP) of a crude oil refinery. By focusing on the degradation of phenol, we observed the involvement of diverse indigenous microbial communities at different steps of the WWTP. The anaerobic bacterial and archaeal genera were replaced by aerobic and facultative anaerobic bacteria through the biological treatment processes. The phyla Proteobacteria, Bacteroidetes and Planctomycetes were dominating at different stages of the treatment. Most of the established protein sequences of the phenol degradation key enzymes belonged to bacteria from the class Alphaproteobacteria. From 35 isolated strains, 14 were able to grow on aromatic compounds, whereas several phenolic compound-degrading strains also degraded aliphatic hydrocarbons. Two strains, Acinetobacter venetianus ICP1 and Pseudomonas oleovorans ICTN13, were able to degrade various aromatic and aliphatic pollutants and were further characterized by whole genome sequencing and cultivation experiments in the presence of phenol to ascertain their metabolic capacity in phenol degradation. When grown alone, the intermediates of catechol degradation, the meta or ortho pathways, accumulated into the growth environment of these strains. In the mixed cultures of the strains ICP1 and ICTN13, phenol was degraded via cooperation, in which the strain ICP1 was responsible for the adherence of cells and ICTN13 diminished the accumulation of toxic intermediates.
Comparative Genomic and Proteomic Analyses of Three Widespread Phytophthora Species: Phytophthora chlamydospora, Phytophthora gonapodyides and Phytophthora pseudosyringae
Authors: McGowan Jamie , O’Hanlon Richard , Owens Rebecca A. , Fitzpatrick David A.
Published: May 2020

The Phytophthora genus includes some of the most devastating plant pathogens. Here we report draft genome sequences for three ubiquitous Phytophthora species—Phytophthora chlamydospora, Phytophthora gonapodyides, and Phytophthora pseudosyringae. Phytophthora pseudosyringae is an important forest pathogen that is abundant in Europe and North America. Phytophthora chlamydospora and Ph. gonapodyides are globally widespread species often associated with aquatic habitats. They are both regarded as opportunistic plant pathogens. The three sequenced genomes range in size from 45 Mb to 61 Mb. Similar to other oomycete species, tandem gene duplication appears to have played an important role in the expansion of effector arsenals. Comparative analysis of carbohydrate-active enzymes (CAZymes) across 44 oomycete genomes indicates that oomycete lifestyles may be linked to CAZyme repertoires. The mitochondrial genome sequence of each species was also determined, and their gene content and genome structure were compared. Using mass spectrometry, we characterised the extracellular proteome of each species and identified large numbers of proteins putatively involved in pathogenicity and osmotrophy. The mycelial proteome of each species was also characterised using mass spectrometry. In total, the expression of approximately 3000 genes per species was validated at the protein level. These genome resources will be valuable for future studies to understand the behaviour of these three widespread Phytophthora species.
Combined Use of Lachancea thermotolerans and Schizosaccharomyces pombe in Winemaking: A Review
Authors: Benito Santiago
Published: May 2020

The combined use of Lachancea thermotolerans and Schizosaccharomyces pombe is a new winemaking biotechnology that aims to solve some modern industrial oenology problems related to warm viticulture regions. These areas are characterized for producing musts with high levels of sugar that can potentially be converted into wines with elevated ethanol contents, which are usually associated with high pH levels. This biotechnology was reported for the first time in 2015, and since then, several scientific articles have been published regarding this topic. These reported scientific studies follow an evolution similar to that performed in the past for Saccharomyces cerevisiae and Oenococcus oeni; they start by reporting results for basic winemaking parameters at the beginning, later continuing with more advanced parameters. This review compares the results of different researchers that have applied this new biotechnology and have studied wine quality parameters such as ethanol, glycerol, malic acid, lactic acid, amino acids, aroma compounds, or anthocyanins. It is shown that the new biotechnology is repeatedly reported to solve specific winemaking problems such as the lack of acidity, biogenic amines, ethyl carbamate, or undesirable color losses. Such results highlight this biotechnology as a promising option for warm viticulture areas.
The Microbiome of Peri-Implantitis: A Systematic Review and Meta-Analysis
Authors: Sahrmann Philipp , Gilli Fabienne , Wiedemeier Daniel B. , Attin Thomas , Schmidlin Patrick R. , Karygianni Lamprini
Published: May 2020

This review aimed to systematically compare microbial profiles of peri-implantitis to those of periodontitis and healthy implants. Therefore, an electronic search in five databases was conducted. For inclusion, studies assessing the microbiome of peri-implantitis in otherwise healthy patients were considered. Literature was assessed for consistent evidence of exclusive or predominant peri-implantitis microbiota. Of 158 potentially eligible articles, data of 64 studies on 3730 samples from peri-implant sites were included in this study. Different assessment methods were described in the studies, namely bacterial culture, PCR-based assessment, hybridization techniques, pyrosequencing, and transcriptomic analyses. After analysis of 13 selected culture-dependent studies, no microbial species were found to be specific for peri-implantitis. After assessment of 28 studies using PCR-based methods and a meta-analysis on 19 studies, a higher prevalence of Aggregatibacter actinomycetemcomitans and Prevotella intermedia (log-odds ratio 4.04 and 2.28, respectively) was detected in peri-implantitis biofilms compared with healthy implants. Actinomyces spp., Porphyromonas spp. and Rothia spp. were found in all five pyrosequencing studies in healthy-, periodontitis-, and peri-implantitis samples. In conclusion, the body of evidence does not show a consistent specific profile. Future studies should focus on the assessment of sites with different diagnosis for the same patient, and investigate the complex host-biofilm interaction.
Walstromite, BaCa2(Si3O9), from Rankinite Paralava within Gehlenite Hornfels of the Hatrurim Basin, Negev Desert, Israel
Authors: Krzątała Arkadiusz , Krüger Biljana , Galuskina Irina , Vapnik Yevgeny , Galuskin Evgeny
Published: May 2020

Walstromite, BaCa2Si3O9, known only from metamorphic rocks of North America, was found in small veins of unusual rankinite paralava within gehlenite hornfelses of the Hatrurim Complex, Israel. It was detected at two localities—Gurim Anticline and Zuk Tamrur, Hatrurim Basin, Negev Desert. The structure of Israeli walstromite [with P 1 ¯ space group and cell parameters a = 6.74874(10) Å, b = 9.62922(11) Å, c = 6.69994(12) Å, α = 69.6585(13)°, β = 102.3446(14)°, γ = 96.8782(11)°, Z = 2, V = 398.314(11) Å3] is analogous to the structure of walstromite from type locality—Rush Creek, eastern Fresno County, California, USA. The Raman spectra of all tree minerals exhibit bands related to stretching symmetric vibrations of Si-O-Si at 650–660 cm−1 and Si-O at 960–990 cm−1 in three-membered rings (Si3O9)6−. This new genetic pyrometamorphic type of walstromite forms out of the differentiated melt portions enriched in Ba, V, S, P, U, K, Na, Ti and F, a residuum after crystallization of rock-forming minerals of the paralava (rankinite, gehlenite-åkermanite-alumoåkermanite, schorlomite-andradite series and wollastonite). Walstromite associates with other Ba-minerals, also products of the residual melt crystallization as zadovite, BaCa6[(SiO4)(PO4)](PO4)2F and gurimite, Ba3(VO4)2. The genesis of unusual barium mineralization in rankinite paralava is discussed. Walstromite is isostructural with minerals—margarosanite, BaCa2Si3O9 and breyite, CaCa2(Si3O9), discovered in 2018.
Archaeological Ceramic Diagenesis: Clay Mineral Recrystallization in Sherds from a Late Byzantine Kiln, Israel
Authors: Weiner Steve , Nagorsky Alla , Feldman Yishai (Isai) , Kossoy Anna
Published: May 2020

The pseudo-amorphous clay components of some of the pottery sherds that formed a surface in the firing chamber of a Late Byzantine kiln were shown by Fourier Transform Infrared Spectroscopy to have undergone almost complete recrystallization. Powder X-ray diffraction showed that the crystalline montmorillonite component of these sherds increased and kaolinite formed de novo. As this recrystallization process only occurred in the center of the firing chamber, we infer that the recrystallization process was due to repeated exposure of the sherds to high temperatures. The zeolite gonnardite was identified by X-ray diffraction. The chemical compositions of sodium-rich minerals, determined by energy dispersive X-ray spectroscopy (EDS), are consistent with the presence of gonnardite and analcime, and showed that the sodium was partially substituted by calcium and other cations. As these zeolites were also present in sherds from the upper pottery chamber, they did not form only as a result of repeated exposure to high temperatures. The demonstration that the clay mineral component of ceramics can undergo diagenetic recrystallization supports the possibility that provenience studies based on elemental analyses, especially of cooking pots that are repeatedly exposed to high temperatures, may be affected by recrystallization.
Water Content in Garnet from Eclogites: Implications for Water Cycle in Subduction Channels
Authors: Gou Yiren , Wang Qin , Li Yan , Wirth Richard
Published: May 2020

Garnet from eclogites often shows very heterogenous and extremely high hydroxyl concentration. Eight eclogite samples were selected from the Sulu ultrahigh-pressure terrane and the Sumdo high-pressure metamorphic belt (Lhasa). The mean hydroxyl concentration in pyrope-rich and almandine-rich garnet varies from 54 to 427 ppm H2O and increases with the retrogression degree of eclogites. TEM observations reveal nanometer-sized anthophyllite exsolutions and clinochlore inclusions in water-rich domains in garnet, where anthophyllite is partly replaced by clinochlore. Because of overlapping of the infrared stretching absorption bands for structural OH in garnet and chlorite, it is impossible to exclude contribution of chlorite inclusions to the estimated hydroxyl concentration in garnet. The broad band near 3400 cm−1 is attributed to molecular water and nanometer-sized chlorite inclusions. Anthophyllite exsolutions may be formed by decomposition of hydrous garnet from ultrahigh-pressure eclogites during exhumation. Significant amounts of water can be stored in garnet from massif eclogites in the forms of hydroxyl in garnet and nanometer-sized inclusions of anthophyllite and clinochlore, as well as fluid inclusions. Amphibolite facies retrograde metamorphism can significantly increase both hydroxyl concentration and water heterogeneity in garnet from massif eclogites. These nano-inclusions in garnet provide a window to trace the water cycle in subduction channels.
Bone Diseases: Current Approach and Future Perspectives in Drug Delivery Systems for Bone Targeted Therapeutics
Authors: Chindamo Giulia , Sapino Simona , Peira Elena , Chirio Daniela , Gonzalez Mónica Cristina , Gallarate Marina
Published: May 2020

Bone diseases include a wide group of skeletal-related disorders that cause mobility limitations and mortality. In some cases, e.g., in osteosarcoma (OS) and metastatic bone cancer, current treatments are not fully effective, mainly due to low patient compliance and to adverse side effects. To overcome these drawbacks, nanotechnology is currently under study as a potential strategy allowing specific drug release kinetics and enhancing bone regeneration. Polymers, ceramics, semiconductors, metals, and self-assembled molecular complexes are some of the most used nanoscale materials, although in most cases their surface properties need to be tuned by chemical or physical reactions. Among all, scaffolds, nanoparticles (NPs), cements, and hydrogels exhibit more advantages than drawbacks when compared to other nanosystems and are therefore the object of several studies. The aim of this review is to provide information about the current therapies of different bone diseases focusing the attention on new discoveries in the field of targeted delivery systems. The authors hope that this paper could help to pursue further directions about bone targeted nanosystems and their application for bone diseases and bone regeneration.
Fitting Biochars and Activated Carbons from Residues of the Olive Oil Industry as Supports of Fe- Catalysts for the Heterogeneous Fenton-Like Treatment of Simulated Olive Mill Wastewater
Authors: Esteves Bruno M. , Morales-Torres Sergio , Maldonado-Hódar Francisco J. , Madeira Luis M.
Published: May 2020

A series of biochars and activated carbons (ACs) was prepared combining carbonization and physical or chemical activation of cheap and abundant residues of the olive oil industry. These materials were used as Fe-support to develop low-cost catalysts for the heterogeneous Fenton-like oxidation of simulated olive mill wastewater (OMW), the highly pollutant effluent generated by this agroindustry. Commercial ACs were also used as reference. All catalysts prepared were extensively characterized and results related with their performances in the catalytic wet peroxide oxidation (CWPO). Results showed a linear relationship of the textural properties of the catalysts with the adsorptive and catalytic performance, as well as the preferential adsorption and degradation of some phenolic compounds (caffeic and gallic acids) by specific interactions with the catalysts’ surface. Despite the best performance of catalysts developed using commercial supports, those prepared from agro-industrial residues present some advantages, including a smaller catalyst deactivation by iron leaching. CWPO results show that catalysts from physically activated olive stones are the most promising materials, reaching total organic carbon and toxicity reductions of 35% and 60%, respectively, as well an efficient use of H2O2, comparable with those obtained using commercial supports. This approach showed that the optimized treatment of this type of residues will allow their integration in the circular economic process of the olive oil production.
The Protective Effect of Rosmarinic Acid against Unfavorable Influence of Methylparaben and Propylparaben on Collagen in Human Skin Fibroblasts
Authors: Matwiejczuk Natalia , Galicka Anna , Zaręba Ilona , Brzóska Małgorzata M.
Published: May 2020

Parabens, which are widely used in food, medicines and cosmetics, have a harmful effect on human health. People are most exposed to parabens transdermally by using cosmetic products containing these preservatives. The purpose of this study was to estimate the influence of parabens (methylparaben—MP and propylparaben—PP) on the metabolism of collagen in the human skin fibroblasts and above all, to assess whether rosmarinic acid (RA—50, 100, or 150 μM) can protect these cells from the adverse effects of parabens (0.001% MP and 0.0003% PP, 0.003% MP and 0.001% PP, and 0.01% MP and 0.003% PP). The possible mechanisms of RA action were estimated as well. Parabens decreased the expression of collagen type I and III at mRNA and protein levels, while RA (depending on the concentration) provided partial or total protection against these changes. The effective protection against the adverse effects of parabens on cell viability and proliferation was also provided by RA. The beneficial impact of RA on collagen and the fibroblasts resulted from an independent action of this compound and its interaction with parabens. This study allows us to conclude that this polyphenolic compound may protect from unfavorable health outcomes caused by lifetime human exposure to parabens contained in cosmetic products.
Change in Growth and Diet Quality Among Preschool Children in Tokyo, Japan
Authors: Shinsugi Chisa , Tani Yukako , Kurotani Kayo , Takimoto Hidemi , Ochi Manami , Fujiwara Takeo
Published: May 2020

Dietary intake of adequate quality and quantity in early life is essential for healthy growth and development. This study aimed to examine the association between one-year change in growth and diet quality in preschool children in Adachi City, Tokyo, Japan. A total of 110 participants (49% boys, four to five years old at baseline) were included in this analysis. Body mass index for age z-score (BAZ) and height for age z-score (HAZ) were calculated in accordance with WHO reference. Dietary intakes were assessed using the brief-type self-administered diet history questionnaire for children (BDHQ3y), and daily quality score was calculated based on the Japanese Food Guide Spinning Top (JFGST score). Regression analyses found no significant association between one-year change in growth and diet quality (compared to a low JFGST score, BAZ: β = 0.16, 95% CI: −0.29 to 0.60 for a middle JFGST score, β = −0.14, 95% CI: −0.61 to 0.33 for a high JFGST score, HAZ: β = −0.15, 95% CI: −0.50 to 0.21 for a middle JFGST score, β = −0.06, 95% CI: −0.43 to 0.30 for a high JFGST score). Further studies are needed to develop an appropriate diet quality index for healthy growth in Japanese preschool children.
A Pilot Screening of Agro-Food Waste Products as Sources of Nutraceutical Formulations to Improve Simulated Postprandial Glycaemia and Insulinaemia in Healthy Subjects
Authors: Tenore Gian Carlo , Caruso Domenico , D’Avino Maria , Buonomo Giuseppe , Caruso Giuseppe , Ciampaglia Roberto , Schiano Elisabetta , Maisto Maria , Annunziata Giuseppe , Novellino Ettore
Published: May 2020

The control of glucose homeostasis is the main goal for both the prevention and management of diabetes and pre-diabetes. Numerous drugs are available, despite their side effects. This is constantly leading people to be inclined to natural alternative treatments. Evidence indicates antioxidant-based nutraceuticals as an optimal tool for the glycaemic control. Currently, a great interest has been focused on the valorisation of agro-food by-products as sources of bioactive compounds including polyphenols. In this sense, we tested the efficacy of novel nutraceutical products based on polyphenolic extract from nectarines (NecP), tomato peels (TP), and olive leaves (EOL) on glycaemic and insulinemic responses. The three formulations contained, respectively, 0.007 mg abscisic acid (ABA)/g, 0.5 mg carotenoids/g, and 150 mg oleuropein/g. Twenty healthy subjects consumed a regular glucose solution (RG) or a treatment beverage (TB) obtained by mixing RG with the individual formulations (TB NecP, TB EOL, and TB TP), separately, and on different days. All three formulations significantly lowered the 30 min glucose plasma peak (p < 0.05 for all); similarly, NecP and TP also significantly lowered the 30 min insulin plasma peak (p < 0.05 for all). These results may lead to the hypothesis of a formulation of a multi-component nutraceutical with a synergistic efficacy for the glycaemic control.
Sarcopenia: A Contemporary Health Problem among Older Adult Populations
Authors: Papadopoulou Sousana
Published: May 2020

Sarcopenia, a geriatric disease characterized by a progressive loss of skeletal muscle mass and loss of muscle function, constitutes a rising, often undiagnosed health problem. Its prevalence in the elderly population is largely considered variable, as it ranges from 5% to 50% depending on gender, age, pathological conditions as well as diagnostic criteria. There is no one unified approach of treatment or assessment, which makes sarcopenia even harder to assess. There is a pressing need to provide better diagnosis, diagnostics, prevention, and individualized health care. Physical activity and nutrition are the main studied ways to prevent sarcopenia, and they also offer better outcomes. This review aims to report the prevalence of sarcopenia in older adults, its etiology, prevention, and treatment techniques.
Self-Reported Eating Speed and Incidence of Gestational Diabetes Mellitus: the Japan Environment and Children’s Study
Authors: Dong Jia-Yi , Ikehara Satoyo , Kimura Takashi , Cui Meishan , Kawanishi Yoko , Kimura Tadashi , Ueda Kimiko , Iso Hiroyasu ,   the Japan Environment and Children’s Study Group
Published: May 2020

There is little evidence linking eating speed to gestational diabetes mellitus (GDM) incidence. We therefore aimed to evaluate the prospective association of eating speed with GDM incidence. Overall, 97,454 pregnant women were recruited between January 2011 and March 2014. Singleton pregnant women who did not have GDM, heart disease, stroke, cancer, type 1 diabetes, and/or type 2 diabetes at the time of study enrollment were eligible. Each woman was asked about her eating speed at that time via a questionnaire. Odds ratios of GDM in relation to eating speed were obtained using logistic regression. Among the 84,811 women eligible for analysis, 1902 cases of GDM were identified in medical records. Compared with women who reported slow eating speed, the age-adjusted odds ratios (95% confidence interval) of GDM for women who reported medium, relatively fast, or very fast eating speed were 1.03 (0.90, 1.18), 1.07 (0.94, 1.23), and 1.28 (1.05, 1.58), respectively. Adjustment for demographic, lifestyle-related, and dietary factors including dietary fat, dietary fiber, and energy intakes yielded similar results. The association was attenuated and no longer significant after further adjustment for pre-pregnancy body mass index. The mediation analysis showed that being overweight accounted for 64% of the excess risk of GDM associated with eating speed. In conclusion, women who reported very fast eating speed, compared with those reporting slow eating speed, were associated with an increased incidence of GDM, which may be largely mediated by increased body fat.
Effects of Arginine Supplementation on Athletic Performance Based on Energy Metabolism: A Systematic Review and Meta-Analysis
Authors: Viribay Aitor , Burgos José , Fernández-Landa Julen , Seco-Calvo Jesús , Mielgo-Ayuso Juan
Published: May 2020

Nitric oxide related ergogenic aids such as arginine (Arg) have shown to impact positively on sport performance through several physiological and metabolic mechanisms. However, research results have shown to be controversial. The great differences regarding required metabolic pathways and physiological demands between aerobic and anaerobic sport disciplines could be the reasons. The aim of this systematic review and meta-analysis was to evaluate the effects of Arg supplementation on aerobic (≤VO2max) and anaerobic (>VO2max) performance. Likewise, to show the effective dose and timing of this supplementation. A structured search was carried out in accordance with PRISMA® (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) statement and PICOS guidelines in PubMed/MEDLINE, Web of Science (WOS), and Scopus databases from inception to January 2020. Eighteen studies were included which compare Arg supplementation with placebo in an identical situation and testing its effects on aerobic and anaerobic performance tests. Trials analyzing supplementation with other supplements were removed and there was not athlete’s level, gender, ethnicity, or age filters. The performed meta-analysis included 15 studies and random effects model and pooled standardized mean differences (SMD) were used according to Hedges’ g. Results revealed that Arg supplementation could improve aerobic (SMD, 0.84; 95% CI, 0.12 to 1.56; magnitude of SMD (MSMD), large; I2, 89%; p = 0.02) and anaerobic (SMD, 0.24; 95% CI, 0.05 to 0.43; MSMD, small; I2, 0%; p = 0.01) performance tests. In conclusion, acute Arg supplementation protocols to improve aerobic and anaerobic performance should be adjusted to 0.15 g/kg of body weight ingested between 60–90 min before. Moreover, chronic Arg supplementation should include 1.5–2 g/day for 4–7 weeks in order to improve aerobic performance, and 10–12 g/day for 8 weeks to enhance anaerobic performance.
Walnut Oil Alleviates Intestinal Inflammation and Restores Intestinal Barrier Function in Mice
Authors: Bartoszek Adrian , Makaro Adam , Bartoszek Agnieszka , Kordek Radzisław , Fichna Jakub , Salaga Maciej
Published: May 2020

Ulcerative colitis belongs to inflammatory bowel diseases, which is a group of chronic disorders of the gastrointestinal tract. It is a debilitating condition with a wide range of symptoms including rectal bleeding, diarrhea, and visceral pain. Current dietary habits often lead to imbalance in n-6/n-3 polyunsaturated fatty acids (PUFA) in favor of n-6 PUFA. Recent data showed the potential anti-inflammatory advantage of n-3 PUFA. Walnut oil (WO) is rich in those fatty acids and mainly consists of linoleic and linolenic acids that may act via free fatty acids receptors (FFARs). We assessed the anti-inflammatory effect of WO in the mouse model of dextran sulfate sodium (DSS)-induced colitis. Moreover, we examined changes in the expression of tight junction proteins (TJ), pro-inflammatory cytokines, and FFAR proteins in the inflamed mouse colon. WO improves the damage score in inflamed tissue, significantly restoring ion transport and colonic wall permeability. Inflammation caused changes in TJ, FFAR, and pro-inflammatory gene proteins expression, which WO was able to partially reverse. WO has anti-inflammatory properties; however, its exact mechanism of action remains unclear. This stems from the pleiotropic effects of n-3 PUFA ligands associated with receptor distribution and targeted signaling pathways.
Exercise and Curcumin in Combination Improves Cognitive Function and Attenuates ER Stress in Diabetic Rats
Authors: Cho Jin Ah , Park Se Hwan , Cho Jinkyung , Kim Jong-Oh , Yoon Jin Hwan , Park Eunmi
Published: May 2020

Type 2 diabetes mellitus (T2DM) is a metabolic disease associated with chronic low-grade inflammation that is mainly associated with lifestyles. Exercise and healthy diet are known to be beneficial for adults with T2DM in terms of maintaining blood glucose control and overall health. We investigated whether a combination of exercise and curcumin supplementation ameliorates diabetes-related cognitive distress by regulating inflammatory response and endoplasmic reticulum (ER) stress. This study was performed using male Otsuka Long-Evans Tokushima Fatty (OLETF) rats (a spontaneous diabetes Type 2 model) and Long-Evans Tokushima Otsuka (LETO) rats (LETO controls) by providing them with exercise alone or exercise and curcumin in combination. OLETF rats were fed either a diet of chow (as OLETF controls) or a diet of chow containing curcumin (5 g/kg diet) for five weeks. OLETF rats exercised with curcumin supplementation exhibited weight loss and improved glucose homeostasis and lipid profiles as compared with OLETF controls or exercised OLETF rats. Next, we examined cognitive functions using a Morris water maze test. Exercise plus curcumin improved escape latency and memory retention compared to OLETF controls. Furthermore, OLETF rats exercised and fed curcumin had lower IL6, TNFα, and IL10 levels (indicators of inflammatory response) and lower levels of ER stress markers (BiP and CHOP) in the intestine than OLETF controls. These observations suggest exercise plus curcumin may offer a means of treating diabetes-related cognitive dysfunction.
Crosslinked Fibroin Nanoparticles: Investigations on Biostability, Cytotoxicity, and Cellular Internalization
Authors: Pham Duy Toan , Saelim Nuttawut , Cornu Raphaël , Béduneau Arnaud , Tiyaboonchai Waree
Published: May 2020

Recently, crosslinked fibroin nanoparticles (FNP) using the crosslinker 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) or the polymer poly(ethylenimine) (PEI) have been developed and showed potentials as novel drug delivery systems. Thus, this study further investigated the biological properties of these crosslinked FNP by labeling them with fluorescein isothiocyanate (FITC) for in vitro studies. All formulations possessed a mean particle size of approximately 300 nm and a tunable zeta potential (−20 to + 30 mV) dependent on the amount/type of crosslinkers. The FITC-bound FNP showed no significant difference in physical properties compared to the blank FNP. They possessed a binding efficacy of 3.3% w/w, and no FITC was released in sink condition up to 8 h. All formulations were colloidal stable in the sheep whole blood. The degradation rate of these FNP in blood could be controlled depending on their crosslink degree. Moreover, no potential toxicity in erythrocytes, Caco-2, HepG2, and 9L cells was noted for all formulations at particle concentrations of < 1 mg/mL. Finally, all FNP were internalized into the Caco-2 cells after 3 h incubation. The uptake rate of the positively charged particles was significantly higher than the negatively charged ones. In summary, the crosslinked FNP were safe and showed high potentials as versatile systems for biomedical applications.
The Fast and the FRDR: Improving Metadata for Data Discovery in Canada
Authors: Turp Clara , Wilson Lee , Pascoe Julienne , Garnett Alex
Published: May 2020

The Federated Research Data Repository (FRDR), developed through a partnership between the Canadian Association of Research Libraries’ Portage initiative and the Compute Canada Federation, improves research data discovery in Canada by providing a single search portal for research data stored across Canadian governmental, institutional, and discipline-specific data repositories. While this national discovery layer helps to de-silo Canadian research data, challenges in data discovery remain due to a lack of standardized metadata practices across repositories. In recognition of this challenge, a Portage task group, drawn from a national network of experts, has engaged in a project to map subject keywords to the Online Computer Library Center’s (OCLC) Faceted Application of Subject Terminology (FAST) using the open source OpenRefine software. This paper will describe the task group’s project, discuss the various approaches undertaken by the group, and explore how this work improves data discovery and may be adopted by other repositories and metadata aggregators to support metadata standardization.
Reassessing Religion and Politics in the Life of Jagjivan Rām
Authors: Friedlander Peter
Published: May 2020

Jagjivan Ram (1908–1986) was, for more than four decades, the leading figure from India’s Dalit communities in the Indian National Congress party. In this paper, I argue that the relationship between religion and politics in Jagjivan Ram’s career needs to be reassessed. This is because the common perception of him as a secular politician has overlooked the role that his religious beliefs played in forming his political views. Instead, I argue that his faith in a Dalit Hindu poet-saint called Ravidās was fundamental to his political career. Acknowledging the role that religion played in Jagjivan Ram’s life also allows us to situate discussions of his life in the context of contemporary debates about religion and politics. Jeffrey Haynes has suggested that these often now focus on whether religion is a cause of conflict or a path to the peaceful resolution of conflict. In this paper, I examine Jagjivan Ram’s political life and his belief in the Ravidāsī religious tradition. Through this, I argue that Jagjivan Ram’s career shows how political and religious beliefs led to him favoring a non-confrontational approach to conflict resolution in order to promote Dalit rights.
Using GIS, Remote Sensing, and Machine Learning to Highlight the Correlation between the Land-Use/Land-Cover Changes and Flash-Flood Potential
Authors: Costache Romulus , Bao Pham Quoc , Corodescu-Roșca Ema , Cîmpianu Cătălin , Hong Haoyuan , Thi Thuy Linh Nguyen , Ming Fai Chow , Najah Ahmed Ali , Vojtek Matej , Muhammed Pandhiani Siraj , Minea Gabriel , Ciobotaru Nicu , Cristian Popa Mihnea , Diaconu Daniel Constantin , Thai Pham Binh
Published: May 2020

The aim of the present study was to explore the correlation between the land-use/land cover change and the flash-flood potential changes in Zăbala catchment (Romania) between 1989 and 2019. In this regard, the efficiency of GIS, remote sensing and machine learning techniques in detecting spatial patterns of the relationship between the two variables was tested. The paper elaborated upon an answer to the increase in flash flooding frequency across the study area and across the earth due to the occurred land-use/land-cover changes, as well as due to the present climate change, which determined the multiplication of extreme meteorological phenomena. In order to reach the above-mentioned purpose, two land-uses/land-covers (for 1989 and 2019) were obtained using Landsat image processing and were included in a relative evolution indicator (total relative difference-synthetic dynamic land-use index), aggregated at a grid-cell level of 1 km2. The assessment of runoff potential was made with a multilayer perceptron (MLP) neural network, which was trained for 1989 and 2019 with the help of 10 flash-flood predictors, 127 flash-flood locations, and 127 non-flash-flood locations. For the year 1989, the high and very high surface runoff potential covered around 34% of the study area, while for 2019, the same values accounted for approximately 46%. The MLP models performed very well, the area under curve (AUC) values being higher than 0.837. Finally, the land-use/land-cover change indicator, as well as the relative evolution of the flash flood potential index, was included in a geographically weighted regression (GWR). The results of the GWR highlights that high values of the Pearson coefficient (r) occupied around 17.4% of the study area. Therefore, in these areas of the Zăbala river catchment, the land-use/land-cover changes were highly correlated with the changes that occurred in flash-flood potential.
Lake Ice-Water Classification of RADARSAT-2 Images by Integrating IRGS Segmentation with Pixel-Based Random Forest Labeling
Authors: Hoekstra Marie , Jiang Mingzhe , Clausi David A. , Duguay Claude
Published: May 2020

Changes to ice cover on lakes throughout the northern landscape has been established as an indicator of climate change and variability, expected to have implications for both human and environmental systems. Monitoring lake ice cover is also required to enable more reliable weather forecasting across lake-rich northern latitudes. Currently, the Canadian Ice Service (CIS) monitors lakes using synthetic aperture radar (SAR) and optical imagery through visual interpretation, with total lake ice cover reported weekly as a fraction out of ten. An automated method of classification would allow for more detailed records to be delivered operationally. In this research, we present an automatic ice-mapping approach which integrates unsupervised segmentation from the Iterative Region Growing using Semantics (IRGS) algorithm with supervised random forest (RF) labeling. IRGS first locally segments homogeneous regions in an image, then merges similar regions into classes across the entire scene. Recently, these output regions were manually labeled by the user to generate ice maps, or were labeled using a Support Vector Machine (SVM) classifier. Here, three labeling methods (Manual, SVM, and RF) are applied after IRGS segmentation to perform ice-water classification on 36 RADARSAT-2 scenes of Great Bear Lake (Canada). SVM and RF classifiers are also tested without integration with IRGS. An accuracy assessment has been performed on the results, comparing outcomes with author-generated reference data, as well as the reported ice fraction from CIS. The IRGS-RF average classification accuracy for this dataset is 95.8%, demonstrating the potential of this automated method to provide detailed and reliable lake ice cover information operationally.
Wideband Waveform Generation Using MDDS and Phase Compensation for X-Band SAR
Authors: Kim Kyeong-Rok , Kim Jae-Hyun
Published: May 2020

This study investigated wideband waveform generation using a field programmable gate array (FPGA) for X-band high-resolution synthetic aperture radar (SAR). Due to the range resolution determined by the bandwidth, we focused on wide bandwidth generation while preserving spectrum quality. The proposed method can generate wide bandwidth using a relatively low system clock. The new approach was designed in Simulink and implemented by very-high-speed-integrated-circuits hardware description language (VHDL). We also proposed a hardware structure in accordance with the proposed method. Signal connections of FPGA and digital analog converter (DAC) are described in the design of the proposed hardware structure. The developed X-band waveform generator using the proposed method output the desired pulse waveform. For the reduction of phase error and improvement of spectrum quality at the X-band, phase error compensation and pre-distortion were applied to the waveform generator. The results of the simulation and the hardware output demonstrate that the variation and standard deviation of the phase error were improved within the frequency spectrum. Accordingly, the proposed method and the developed waveform generator have the potential to produce a high-resolution image of the area of interest.