bluuebunny/crossref_metadata_2025 huggingface dataset:
OSTEOPOROSIS SCREENING TECHNOLOGY APPLICATION TO IMPROVE HEALTHY LIFESTYLES OF SCHOOL RESIDENTS IN PORONG, SIDOARJO
Authors: Muqmiroh Lailatul , Kartika Sari Amillia , Devianti Putri Berliana , Muhaimin Muhaimin
Published: June 2022

Background : Osteoporosis is one of degenerative disease that can affect elderly and children as well. Knowledge about Osteoporosis has not been fully understood by school residents and their community.  Objectives : This activity aims to (1) increase the school residents knowledge about Osteoporosis, (2) provide digital health communication media that can be used widely and easily by school residents, (3) increase the ability of school residents to use osteoporosis screening technology application and disseminate to the community. Method : Training and mentoring using ATOs-A application media and pocket books. Monitoring and evaluation is carried out to see and assist teachers in using the Osteoporosis screening application. Knowledge improvement is measured by pre-post test using questionnaire as a tool. Result : There is improvement of school residents knowledge about Osteoporosis and how to use screening technology application. So that, they have more encouragement to improve their knowledge and propagate it to community. Conclusion : Community service has a positive response from school residents and benefits to improve the healthy lifestyle in that area.
FAMILY NURSING CARE OF PHYSICAL MOBILITY DISORDERS IN PATIENT WITH POST STROKE IN BLULUK VILLAGE, LAMONGAN REGENCY
Authors: Iswatun Iswatun , Endah sri Wijayanti  , Rizkika Putri Silvia
Published: June 2022

Introduction: Stroke is brain damage due to blood supply to the brain, an event that suddenly takes place within 24 hours without realizing it, such as rupture of blood vessels in the brain and lack of blood supply in the brain. Prevalence by sex male 11.0% (10.5-11.5 per mile) 355,726 cases, female sex 10.9 (10.4-11.4 per mile) 358,056 cases. The proportion of stroke control to health care facilities in the population aged 15 years with stroke prevalence in East Java routinely 40.0% sometimes 39.5% not re-checking 20.5%. The purpose of this study was to carry out Family Nursing Care In Post Stroke Cases With Nursing Problems Of Physical Mobility Disorders. Methods: This research method uses a case study. Data were collected by means of interviews, observations, and documentation studies. Results: The results of the case studies indicate that there are gaps in the assessment of nursing care. Nursing diagnoses according to the IDHS lead to the main priority, namely Mobility Support (D.0045), priority nursing actions according to SIKI Mobilization Support (I.05173), with an outcome according to the SLKI Physical mobility (L.05042), evaluation of the main priority diagnoses, namely impaired mobility partially resolved by home visits for 6 days. Conclusions: Intervention combination Active and passive Range Of Movement techniques are very important for stroke patients. The family can motivate and teach the client to do this.
THE RELATIONSHIP BETWEEN NURSES’ KNOWLEDGE AND COMPLIANCE IN PERFORMING FALL RISK RE-ASSESSMENT
Authors: Sulistyowati Arlina Dhian , Rusminingsih Esri , Sari Devi Permata , Kayati Fitriana Noor , A Yuli Widhi
Published: June 2022

Introduction: The fall incidence will have a detrimental impact on the hospital, staffs and patients as the service recipients. This can be prevented by conducting fall risk reassessment. It is an assessment of patient’s fall risk carried out by nurses. This study aims to determine the level of knowledge and compliance of nurses in carrying out a patient fall risk reassessment in the inpatient ward. Methods: This study used an observational analytical design with a quantitative method and a cross-sectional approach. A total sampling of 48 respondents were used as samples. Kendall Tau-b test was employed as the analysis technique. Results: The respondents were mostly female or as many as 33 people (68.8%). The educational level of the respondents was mostly D-III or as many as 32 people (66.7%). The majority of respondents’ working period was 3-5 years or as many as 26 people (54.2 %). The average age of the respondents was 27.5 years, the respondents' knowledge was mostly good or as many as 24 people (50%), and the respondents’ compliance in carrying out the fall risk reassessment was mostly compliant or as many as 41 people (85.4%). This study utilized the Kendall Tau-b test and obtained p value = 0.006 and Correlation Coefficient of 0.387.  Conclusions: There is a significant relationship between nurses' knowledge of fall risk and their compliance in carrying out a fall risk reassessment in the inpatient ward of the PDHI Yogyakarta Islamic Hospital. The higher the knowledge, the higher the level of nurse compliance.
NURSING CARE OF CONTINUOUS URINARY INCONTINENCE IN THE ELDERLY POST-ISCHEMIC STROKE A CASE REPORT
Authors: Tyas Anestasia Pangestu Mei , Arisandy Fatimahndya Maulitha De , Ilkafah Ilkafah , Mardhika Amellia , Sulpat Emuliana
Published: June 2022

Introduction : Continuous urinary incontinence is the continued and uncontrolled passing of urine without distension and a feeling of fullness in the bladder due to neurological dysfunction. This condition is often found in the elderly post-ischemic stroke. The study aimed to describe nursing care of continuous urinary incontinence in the elderly post-ischemic stroke in Lamongan. Methods : This study used a case study approach (case report) on a participant through the nursing process approach. The sample in this study is one patient, an elderly post-ischemic stroke. This research was conducted in 2021 in Lamongan with three visits to patients' homes for seven days. Data collection techniques include interviews, observation, and physical examination. This research instrument uses the gerontic nursing care format and sandvik severity index. Results : Continuous urinary incontinence nursing care with the main intervention for urinary incontinence treatment (I.04163) and the supporting intervention is pelvic muscle exercises (I.07215) can be applied to elderly post-ischemic stroke according to the expected outcomes. The continuous urinary incontinence has a partially resolved on the third home visit. The client can hold urine several times when the bladder feels full, reduced the frequency of night urination, and rarely wakes up because of wet diapers. Sandvik Severity Index shows a value of 4 (moderate incontinence). Conclusions: Improving nursing care need for collaboration between patients, families, and health workers in performing pelvic muscle exercises independently, continuously, and regularly to increase the strength of the detrusor-sphincter muscles and showed improvement in urinary continence.
Omics-based integrated analysis identified ATRX as a biomarker associated with glioma diagnosis and prognosis
Authors: Xie Yingbin
Published: June 2022

Objective ATRX is a multifunctional protein that is tightly regulated by and implicated in transcriptional regulation and chromatin remodeling. Numerous studies have shown that genetic alterations in ATRX play a significant role in gliomas. This study aims to further determine the relationship between ATRX and glioma prognosis and identify possible mechanisms for exploring the biological significance of ATRX using large data sets. Methods We used The Cancer Genome Atlas (TCGA) database and 130 immunohistochemical results to confirm the difference in ATRX mutations in high- and low-grade gliomas. An online analysis of the TCGA glioma datasets using the cBioPortal platform was performed to study the relationship between ATRX mutations and IDH1, TP53, CDKN2A and CDKN2B mutations in the corresponding TCGA glioma dataset. In combination with clinical pathology data, the biological significance of the relationships were analyzed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses and annotations of all adjacent genes in the network were performedin the Database for Annotation, Visualization and Integrated Discovery (DAVID) and R language. A protein-protein interaction (PPI) network was constructed, and the interactions of all adjacent nodes were analyzed by the String database and using Cytoscape software. Results In the selected TCGA glioma datasets, a total of 2,228 patients were queried, 21% of whom had ATRX alterations, which co-occurred frequently with TP53 and IDH1 mutations. ATRX alterations are associated with multiple critical molecular events, which results in a significantly improved overall survival (OS) rate. In low-grade gliomas, ATRX mutations are significantly associated with multiple important molecular events, such as ZNF274 and FDXR at mRNA and protein levels. A functional cluster analysis revealed that these genes played a role in chromatin binding and P53, and a link was observed between ATRX and IDH1 and TP53 in the interaction network. ATRX and TP53 are important nodes in the network and have potential links with the blood oxygen imbalance. Conclusions ATRX mutations have clinical implications for the molecular diagnosis of gliomas and can provide diagnostic and prognostic information for gliomas. ATRX is expected to serve as a new therapeutic target.
The correlation and overlaps between PD-L1 expression and classical genomic aberrations in Chinese lung adenocarcinoma patients: a single center case series
Authors: Wu Jianghua , Sun Wei , Wang Haiyue , Huang Xiaozheng , Wang Xinyu , Jiang Weiyang , Jia Ling , Wang Ping , Feng Qin , Lin Dongmei
Published: June 2022

Objective To investigate the correlation and overlaps between PD-L1 expression and classical genomic aberrations in Chinese lung adenocarcinoma (LADC) patients. Methods We reviewed 428 consecutive, surgically resected cases of LADC from October 2015 to December 2016 from our center. PD-L1 expression was evaluated based on tumor proportion score (TPS). Correlation and co-occurrence of PD-L1 expression level with those of classical driver genes, such as EGFR, ALK, ROS-1, and KRAS and with clinical variables and disease-free survival (DFS) were analyzed. Results Seventy of the 428 cases (16.4%) showed TPS = 1%, and 21 cases (4.9%) showed TPS = 50%. PD-L1 positive expression was significantly associated with male gender, smoking, advanced TNM stage, and solid histologic subtype. Both TPS = 1% and = 50% were correlated with the absence of an EGFR mutation (P < 0.001) and the presence of ALK rearrangement ( P = 0.024). KRAS mutation was associated with TPS = 50% (P = 0.035). PD-L1 positivity commonly overlapped with the alterations of classical driver oncogenes (58.5% with TPS = 1% and 42.9% with TPS = 50%). Approximately three-quarters of PD-L1 positive cases co-occurred with classical therapeutic-gene aberrations in cases with stage III/IV cancer or cancer progression. LADC could be divided into four subgroups based on the expression profile of current routine biomarkers for potential therapeutic strategies. Conclusions PD-L1 expression is not only closely correlated with classic gene alterations but also commonly overlaps with the aberrations of classical driver oncogenes in Chinese LADC patients. These findings provide a useful overview of clinical strategies that rely on the profile of routinely used molecular biomarkers.
Interpretation of breast cancer screening guideline for Chinese women
Authors: Huang Yubei , Tong Zhongsheng , Chen Kexin , Wang Ying , Liu Peifang , Gu Lin , Liu Juntian , Yu Jinpu , Song Fengju , Zhao Wenhua , Shi Yehui , Li Hui , Xiao Huaiyuan , Hao Xishan
Published: June 2022

Breast cancer is the most common malignant tumor in Chinese women. Early screening is the best way to improve the rates of early diagnosis and survival of breast cancer patients. The peak onset age for breast cancer in Chinese women is considerably younger than those in European and American women. It is imperative to develop breast cancer screening guideline that is suitable for Chinese women. By summarizing the current evidence on breast cancer screening in Chinese women, and referring to the latest guidelines and consensus on breast cancer screening in Europe, the United States, and East Asia, the China Anti-Cancer Association and National Clinical Research Center for Cancer (Tianjin Medical University Cancer Institute and Hospital) have formulated population-based guideline for breast cancer screening in Chinese women. The guideline provides recommendations on breast cancer screening for Chinese women at average or high risk of breast cancer according to the following three aspects: age of screening, screening methods, and screening interval. This article provides more detailed information to support the recommendations in this guideline and to provide more direction for current breast cancer screening practices in China.
Preclinical Immunogenicity and Efficacy of a Multiple Antigen-Presenting System (Maps) Sars Cov-2 Vaccine
Authors: Brian Cieslewicz , Makrinos Daniel , Burke Heidi , Bree Dara , Haridas Renuka , Tonkiss Ian , Bartsch Yannic , Alter Galit , Malley Richard , Besin Gilles
Published: June 2022

Despite the remarkable success of SARS CoV-2 vaccines, the rise of variants, some of which are more resistant to the effects of vaccination, highlights the potential need for additional COVID-19 vaccines. We used the Multiple Antigen Presenting System (MAPS) technology, in which proteins are presented on a polysaccharide polymer to induce antibody, Th1, Th17 and CD8+ T cell responses, to engineer a novel vaccine targeting SARS CoV-2. This vaccine contains a fragment of the spike (S) protein receptor-binding domain (RBD) sequence of the original D614G strain and was used to immunize nonhuman primates (NHP) for assessment of immunological responses and protection against SARS CoV-2 challenge. The SARS CoV-2 MAPS vaccine generated robust neutralizing antibodies as well as Th1, Th17 and cytotoxic CD8 T-cell responses in NHPs. Furthermore, MAPS-immunized NHPs had significantly lower viral loads in the nasopharynx and lung compared to control animals. Taken together, these findings support the use of the MAPS platform to make a SARS CoV-2 vaccine. The nature of the platform also could enable its use for the inclusion of different variants in a single vaccine.
SARS-CoV-2 Shielding Effect of Chitosan Derivatives: An in-silico Prospective
Authors: Das Poonam , Sahoo Sabuj , Majhi Sanatan , Kerry George rout , Jena Atala Bihari
Published: June 2022

Abstract The present work was designed to investigate antiviral potential of novel mono and oligomeric Chitosan derivatives through in-silico approaches to find potent broad-spectrum anti-viral and promising drug candidates against SARS-CoV-2 and augmentation of their mode of action. Chitosan biopolymer and its derivatives were screened virtually against spike glycoprotein and human ACE2 receptor of nCoV-19. HTCC a polymeric Chitosan has been reported to interact with the corona viral Spike (S) protein and blocks its interaction with ACE2 receptor. Better biocompatibility, structural correlations, variation in degree of decetylation and molecular weight of modified Chitosan derivatives are the key attributes for enhancement of anti-viral activity. The Chitosan derivatives constructively interact with viral S protein. N-Carboxy methyl Chitosan (NCMC) among the Chitosan derivatives displayed efficient binding affinity. NCMC, when compared to mHTCC, a monomeric Chitosan for its interaction with the S protein, RBD site and ACE2 receptor, displayed efficient binding affinity with binding energy of -7.9, -6.3 and -7.4; -6.2, -4.8 and -5.5 Kcal/mol respectively. The interaction of S protein with ACE2 and ligand mHTCC-S protein complex and NCMC-S protein complex with ACE2 was calculated through flexible docking resulted in efficient reduction of binding energy from -901.2kJ/mol to -765.06kJ/mol and-814.72kJ/mol respectively, elucidated that the binding affinity of the viral S protein for its receptor ACE2 decreases in the presence of NCMC/mHTCC. The computational study envisages the antiviral efficiency of NCMC, mHTCC and biocompatible Chitosan derivatives for the first time,as preventive intervention against Covid-19.
Augmented Reality to Engage Visitors of Science Museums through Interactive Experiences
Authors: Spadoni Elena , Porro Sara , Bordegoni Monica , Arosio Ilaria , Barbalini Laura , Carulli Marina
Published: June 2022

In the last years, interactive exhibitions based on digital technologies are becoming widely common, thanks to their flexibility and effectiveness in engaging visitors and creating memorable experiences. One of the topics in which digital technologies can be particularly effective is the communication of abstract concepts that are difficult for the human mind to imagine. An emblematic example is the astronomy discipline, which requires us to imagine and understand phenomena far away from our everyday life. In this paper, the authors present a research project, MARSS, in which digital technologies are used effectively to enhance the Users’ Experience of the Museo Astronomico di Brera located in Milan. Specifically, the MARSS project aims at designing and developing a new digital journey inside the museum to allow different categories of visitors to enjoy the exhibition in an engaging and interactive way. The paper presents the design and development phases of the experience and its evaluation with users. The results of the evaluation indicate that the digital interactive experience has been appreciated by users and is successful in translating the content of high scientific value into more engaging and easily understandable elements.
Virulent Phages Isolated from A Smear-Ripened Cheese are Also Detected in Reservoirs of the Cheese Factory
Authors: Paillet Thomas , Lossouarn Julien , Figueroa Clarisse , Midoux Cédric , Rué Olivier , Petit Marie-Agnès , Dugat-Bony Eric
Published: June 2022

Smear-ripened cheeses host complex microbial communities that play a crucial role in the ripening process. Although bacteriophages have been frequently isolated from dairy products, their diversity and ecological role in such type of cheese remain underexplored. In order to fill this gap, the main objective of this study was to isolate and characterize bacteriophages from the rind of a smear-ripened cheese. Thus, viral particles extracted from cheese rind were tested against a collection of bacterial isolates through a spot assay. In total, five virulent bacteriophages infecting Brevibacterium aurantiacum, Glutamicibacter arilaitensis, Leuconostoc falkenbergense and Psychrobacter aquimaris species were obtained. All exhibit a narrow host range, being only able to infect a few cheese-rind isolates within the same species. The complete genome of each phage was sequenced using both Nanopore and Illumina technologies, assembled and annotated. Sequence comparison with known phages revealed that four of them may represent at least new genera. The distribution of the five virulent phages into the dairy-plant environment was also investigated by PCR and three potential reservoirs were identified. This work provides new knowledge on the cheese rind viral community and an overview of the distribution of phages within a cheese factory.
The Impact of COVID-19 on Dental Treatment in Kuwait – a Retrospective Analysis from the Nation’s Largest Hospital
Authors: AlHayyan Wasmiya Ali , AlShammari Khalaf , AlAjmi Falah , Pani Sharat Chandra
Published: June 2022

Background: The COVID-19 pandemic has changed the way dentistry has been practiced world over , this study sought to assess the impact of the COVID-19 pandemic on the patterns of attendance for dental treatment in a large hospital in Kuwait compare them to data from the year prior to the pandemic Methods: A total of 176,690 appointment records of 34,250 patients presenting to the AlJahra specialist hospital, Kuwait for dental treatment from April 2019 to March 2021 were analyzed. Types of procedures and the departments to which they presented were analyzed and the patterns of attendance before and during the pandemic were compared; Results: While there was a significant reduction in the number of orthodontic, endodontic and periodontal procedures there was no impact on oral surgery, restorative procedures or pediatric dentistry; Conclusions: There has been a return in the numbers of patients availing dental treatment, however, there has been a definite shift in the use of certain dental procedures .
An Advanced Approach for MgZnAl-LDH Catalysts Synthesis Used in Claisen-Schmidt Condensation
Authors: Zăvoianu Rodica , Mihăilă Silvana-Denisa , Cojocaru Bogdan , Tudorache Madalina , Pârvulescu Vasile I. , Pavel Octavian Dumitru , Oikonomopoulos Solon , Jacobsen Elisabeth Egholm
Published: June 2022

Using of the organic base tetramethylammonium hydroxides (TMAH) is a viable, cheap and fast route, for the MgZnAl-LDH type materials synthesis by both co-precipitation and mechano-chemical methods. TMAH provided several advantages as smaller quantity of water required in the washing step compared to the use of inorganic alkalis, prevention of LDH contamination with alkali cations, acting as template molecule in texture tailoring along with disadvantages as its presence in small quantities in the resulting layered materials. Regardless the use of organic / inorganic bases and co-precipitation / mechano-chemical methods, zincite stable phase was found in all the synthesized solids. The basicity of catalysts followed the trend: mixed oxides > reconstructed > parent LDH. The memory effect of LDH is supported only by the presence of Mg and Al cations, while Zn remains as zincite stable phase. The catalytic activities for Claisen-Schmidt condensation of benzaldehyde with cyclohexanone provided values higher than 90% after 2h, with a total selectivity in 2,6-dibenzylidenecyclohexanone, while in self-condensation of cyclohexanone no more than 7.29% after 5h. These behaviors depended on catalysts basicity as well as the planar rigidity of the compound.
Identification of Fish Community in Lake La Alberca, Cienega de Chapala, Michoacán, México
Authors: Catalán-Romero Juan Manuel , Buelna-Osben Héctor René
Published: June 2022

Small, enclosed lake is in a tourist area called "Los Negritos", by the prominences a few steps from the lake on a perimeter of tens of meters with hot pools and mud bubbles. The lake originally belonged to Lake Chapala, so the group of fish present here derives from that. This is an endorheic vessel with at least one deep water inlet to the center, with a slight salinity (2.14 to 2.38 0/00), which is precisely greater at the point of upwelling and at a depth of approximately 36 m. This study to date has continued to collect and take data, also presents the characteristic of being an area of saline soils and halophilic vegetation. This body of water we consider is as relevant as that of Alchichica in Puebla. Its study is by monthly sampling with “chinchorro” and "chango" type trawl, recording morphological parameters, also environmental variables in the water, with a Data Sonde 4 brand Hydrolab. Knowing the structure and temporal dynamics of this fish community, along with the physical and chemical characterization of the water and implementa-tion of Importance Value Index (IVI).
A Comparison of Computer-Aided Diagnosis Schemes Optimized Using Radiomics and Deep Transfer Learning Methods
Authors: Danala Gopichandh , Maryada Sai Kiran , Islam Warid , Faiz Rowzat , Jones Meredith , Qiu Yuchen , Zheng Bin
Published: June 2022

Objective: Radiomics and deep transfer learning are two popular technologies used to develop computer-aided detection and diadnosis (CAD) schemes of medical images. This study aims to investigate and compare advantages and potential limitations of applying these two technologies in developing CAD schemes. Methods: A relatively large and diverse retrospective dataset including 3,000 digital mammograms is assembled in which 1,496 images depict malignant lesions and 1,054 images depict benign lesions. Two CAD schemes are developed to classify breast lesions. The first scheme is developed using four steps namely, applying an adaptive multi-layer topographic region growing algorithm to segment lesions, computing initial radiomics features, applying a principal component algorithm to generate an optimal feature vector, and building a support vector machine classifier. The second CAD scheme is built based a pre-trained residual net architecture (ResNet50) as a transfer learning model to classify breast lesions. Both CAD schemes are trained and tested using a 10-fold cross-validation method. Several score fusion methods are also investigated to classify breast lesions. CAD classification performance is evaluated by the area under ROC curve (AUC). Results: ResNet50 model-based CAD scheme yields AUC = 0.85±0.02, which is significantly higher than radiomics feature-based CAD scheme with AUC = 0.77±0.02 (p < 0.01). Additionally, fusion of classification scores generated by two CAD schemes does not further improve classification performance. Conclusion: This study indicates that using deep transfer learning is more efficient to develop CAD schemes and enables to yield higher lesion classification performance than CAD schemes developed using radiomics-based technology.
Explainable Machine Learning Approaches to Assess the COVID-19 Vaccination Uptake: Social, Political, and Economic Aspects
Authors: Ahmed Jishan , Green Robert , Alauddin Md. , Jaman Md. Hasnat , Saha Goutam
Published: June 2022

COVID-19 vaccine hesitancy is considered responsible for the lower rate of acceptance of vaccines in many parts of the world. However, sources of this hesitancy are rooted in many social, political, and economic factors. This paper strives to find the most important variables in predicting the COVID-19 vaccination uptake. We introduce an explainable machine learning (ML) framework to understand the COVID-19 vaccination uptake around the world. To predict vaccination uptake, we have trained a random forest (RF) regression model using a number of sociodemographic and socioeconomic data. The traditional decision tree (DT) regression model is also implemented as the baseline model. We found that the RF model performed better than the DT model since RF is more robust to handle nonlinearity and multi-collinearity. Also, we have presented feature importance based on impurity measure, permutation, and Shapley values to provide the most significant unbiased features. It is found that electrification coverage and Gross Domestic Product are the strongest predictors for higher vaccination uptake, whereas the Fragile state index (FI) contributed to lower vaccination uptake. These findings suggest addressing issues that are found responsible for lower vaccination uptake to combat any future public health crisis.
The Chemical Literacy Understanding of Chemistry Teachers at Islamic Senior High School
Authors: Alwathoni Mahbub , Saputro Sulistyo , Yamtinah Sri , Masykuri Mohammad
Published: June 2022

Chemical literacy has a role in helping students understand local and global issues such as the energy crisis, environment, and health. Teachers need an adequate understanding of chemical literacy to plan appropriate learning and design adequate assessment instruments. This study aims to analyze the description of chemical literacy skills in chemistry teachers at Madrasah Aliyah (Islamic Senior High School). This research is a quantitative descriptive method that describes the understanding of chemical literacy. A total of 85 teachers from 12 Madrasah Aliyah in Central Java, Indonesia were respondents. The research data were obtained by questionnaire with a total of 24 statements about the components of chemical literacy. Questionnaire analysis carried out to see the relationship between teacher understanding in assessing chemical literacy, obtained by using RASCH analysis. The results showed that the reliability of the questionnaire was 0.98. Cronbach's Alpha (α) which measures the interaction between respondents and items has a value of 0.96. This value indicates the instrument used is in perfect condition and effective with a high level of reliability so that it can use in actual research. The results showed that the teachers had not fully understood the domains and aspects of chemical literacy skills. The Wright map shows only 30% of respondents (teachers) understand the aspects of chemical literacy and apply them in chemistry learning. The results of this study suggest that policymakers such as the government and educational institutions, hold more in-house training and workshops on chemical literacy for teachers.
Effective Management of Non-Surgical Periodontal Treatment in a Patient with Severe Gingival Enlargement and Periodontal Bone Loss: A Case Report
Authors: Dalipi Zana S , Dragidella Fatmir , Dragidella Donika K
Published: June 2022

The immune-inflammatory response of periodontal tissue can be influenced by various systemic variables such as hormone disorders, hematologic diseases, nutrition, and medications. Inflammatory cytokines released by infected periodontal tissue are implicated in suppressing erythrocyte growth, differentiation, and erythropoiesis inhibition. We present a case of non-surgical periodontal treatment in a 31-year-old female patient with severe gingival enlargement associated with aggressive bone destruction combined with iron deficiency anemia (IDA). The periodontal treatment plan was achieved in collaboration with the hematologist, starting with oral hygiene instruction, scaling and root planning (SRP) by sextants with systemic antibiotic therapy combined with a chemical agent and low-level-laser-therapy (LLLT), and extraction of hopeless teeth. Effective non-surgical periodontal treatment with SRP and LLLT reduces inflammation and gingival enlargements and controls the recolonization of subgingival sites by periodontal pathogens as a crucial factor to proceed with periodontal surgery. Function, aesthetics, and the patient’s social communication are all improved.
Prevalence and Characteristics of Impacted Third Molars in a Kosovar Population: A Cross-Sectional Study
Authors: Dalipi Zana Sllamniku , Koҁani Ferit K , Sejfija Zana O
Published: June 2022

Background: The goal of this randomized cross-sectional study was to determine the prevalence of impacted third molars in the Kosovar population and to assess the site of impaction using the Winter and the Pell and Gregory classifications. Methods and Results: A total of 5515 digital panoramic radiographs (PANs) were reviewed to determine the number of impacted third molars, their angulation, and depth. The age range was from 18 to 77 years (29.3±12.8 years). The prevalence of impacted third molars in the sample of the Kosovar population was 73.7%. Of all PANs, one or more impacted third molars were observed in a total of 710 PANs, including 296(41.7%) in men and 414(58.3%) in women, with a sex ratio of 1:1.4; this difference was not significant (P=0.616). The mandible exhibited a substantially greater prevalence of impacted third molars than the maxilla 62.1% vs. 37.9%; P=0.001). In the mandible, significantly higher frequencies of third molar impaction were noted in the distoangular and vertical positions (36.9% and 33.5%). In the maxilla, the most common angulation of impacted third molars was mesioangular (52.8%). Most impacted third molars recorded a Class C depth of impaction (62.3%) followed by Class B (34.9%), and only 2.8% recorded a Class A depth of impaction. Conclusion: The samples of third molar impaction in the Kosovar population are characterized by a high prevalence of impaction, especially in the mandible. The most common angulation was the mesial in the maxilla as well as in the mandible. The most common level of impaction in the maxilla and mandible was level C.
Oxidative Stress Intensity in Children and Adolescents with a New Coronavirus Infection
Authors: Rychkova Lyubov V , Darenskaya Marina A , Semenova Natalya V , Kolesnikov Sergey I , Petrova Alla G , Nikitina Olga A , Brichagina Anastasia S , Kolesnikova Lyubov I
Published: June 2022

The aim of our research was to assess the intensity of oxidative stress (OS) in children and adolescents with COVID-19 using the oxidative stress index (OSI). Methods and Results: The study was conducted between May 2020 and March 2021. The main group included 17 children and adolescents [8(47.1%) boys and 9(52.9%) girls; mean age of 12.35±4.01 years] with diagnosed COVID-19 infection (mild to moderate course) selected as a result of the primary diagnostic examination from among those admitted to hospitalization at the Irkutsk Regional Infectious Diseases Clinical Hospital. The control group included 17 healthy children and adolescents (average age of 12.35±4.01 years) matched by copy-pair type. The obtained data indicated statistically significant differences in a number of parameters between children and adolescents with COVID-19 and the control groups. We found statistically significant higher levels of LPO products (CDs, P<0.0001; KD and CT, P=0.006; and TBARs, P=0.013) in the study group than in the control group. Among AOD system parameters, the levels of retinol (P=0.015) and reduced glutathione (P=0.048) and SOD activity (P<0.0001) were statistically lower in the study group than in the control group. The OSI level was significantly greater (by 8.5 times, P=0.028) in the study group than in the control group, which confirms the development of antioxidant deficiency in COVID-19. Conclusion: The results of the assessment of OSI in children and adolescents with COVID-19 indicate insufficient activity of some critical components of AOD and a shift of the redox balance toward pro-oxidant factors, which can have extremely negative consequences in the development of the disease. In this regard, we recommend carrying out corrective measures to stabilize LPO/AOD parameters by including drugs with antioxidant properties in the treatment complex.
Mast Cells as the Target of the Biological Effects of Molecular Hydrogen in the Specific Tissue Microenvironment
Authors: Atiakshin Dmitry A , Shishkina Victoria , Esaulenko Dmitry I , Ovsyannikov Evgeniy S , Antakova Lyubov N , Gerasimova Olga A , Samoilenko Tatiana V , Andreev Pavel Yu , Magerramova Sara T , Budnevskaya Sofia A
Published: June 2022

Mast cells (MCs) as key players in the development of both physiological and pathological processes in the organism can form a specific tissue microenvironment. Having a rich secretion of biologically active substances, MCs can secrete tryptase and/or chymase and thereby participate in the regulation of processes such as inflammation, neoangiogenesis, allergic reactions, and oncogenesis. Reactive oxygen intermediates (ROI) play an essential role in regulation of MC degranulation, shown in vitro and in vivo models. Application of molecular hydrogen as a substance with antioxidant characteristics pathogenically appears to be an important mechanism decreasing MC secretory activity, and, as a consequence, a novel option to reduce an inflammatory background in the specific tissue microenvironment.
Análise musical de canções em aulas de português como língua materna, na perspectiva de Copland
Authors: Nishida Gustavo
Published: June 2022

O objetivo deste trabalho é apresentar alguns elementos musicais que possam servir de guia para o trabalho docente com as canções populares em aulas de português como língua materna. As orientações se baseiam na perspectiva de Copland sobre como ouvimos música. Além disso, é apresentada uma estrutura analítica da canção popular para que o professor consiga entender como o gênero canção se organiza. Por fim, apresenta-se uma análise da canção “Marchinha psicótica de Doutor Soup” de Júpiter Maçã para exemplificar como uma análise pode ser realizada com esses princípios. A análise mostra que os efeitos de sentidos são entendidos a partir do reconhecimento de estruturas musicais que não fazem parte do gênero marchinha.
Endophytic bacterium Bacillus aryabhattai induces novel transcriptomic changes to stimulate plant growth.
Authors: Xu Hongli , Gao Jingyao , Portieles Roxana , Du Lihua , Gao Xiangyou , Borrás-Hidalgo Orlando
Published: June 2022

In nature, plants interact with a wide range of microorganisms, and most of these microorganisms have the ability to promote plant growth through the induction of important molecular pathways. The current study evaluated whether the endophytic bacterium Bacillus aryabhattai encourages plant growth and the transcriptional changes that might be implicated in this effect. The endophytic bacterium promotes the growth of Arabidopsis and tobacco plants. The transcriptional changes in Arabidopsis plants treated with the bacterium were also identified, and the results showed that various genes, such as cinnamyl alcohol dehydrogenase, apyrase, thioredoxin H8, benzaldehyde dehydrogenase, indoleacetaldoxime dehydratase, berberine bridge enzyme-like and gibberellin-regulated protein, were highly expressed. Also, endophytic bacterial genes, such as arginine decarboxylase, D-hydantoinase, ATP synthase gamma chain and 2-hydroxyhexa-2,4-dienoate hydratase, were activated during the interaction. These findings demonstrate that the expression of novel plant growth-related genes is induced by interaction with the endophytic bacterium B. aryabhattai and that these changes may promote plant growth in sustainable agriculture.
In-vitro Cytotoxicity and anticancer drug release behavior of Methionine-coated magnetite nanoparticles as carriers.
Authors: Yeganeh Faten Eshrati , Yeganeh Amir Eshrati , Fatemizadeh Mahdi , Far Bahareh Farasati Farasati , Quazi Sameer , Safdar Muhammad
Published: June 2022

A novel and specific Drug delivery for in vitro cancer-targeted are developed successfully by a simple one-step method. A CoFe2O4@Methionine core-shell nanoparticle was prepared by the reflux assay which amino acid in the surface makes ferrite biocompatible, enhances its chemical stability, and improves the drug loading capacity. The synthesized nanoparticles were characterized using FTIR, TGA, XRD, SEM, TEM, and VSM which coating amino acid on the surface of CoFe2O4 was confirmed by XRD and TGA. The appearance of a new peak for C≡N confirms the formation of Letrozole loaded carrier in the FTIR. The vibrating sample magnetometer of both bare CoFe2O4 and Methionine-coated CoFe2O4 nanoparticles exhibited room-temperature superparamagnetic behavior with a saturation value of 46emu/g and 16.8emu/g, respectively. The morphology and size of samples were characterized by SEM and TEM that the average size of the particle was around 28–29 nm. The loading of Letrozole and the effect of pH (5, 7.4) on the release behavior of the carrier was studied. The result of the drug release in pH equals 5 was about 88% which higher than pH equals 7.4. Also, the preparation had been evaluated for determining its cytotoxicity using MCF-7, MDA-MB-231, and MCF10A cells as an in vitro model, and the result vitro experiments showed that CoFe2O4@Methionine could significantly reduce cancer in cells model. These results demonstrate that core-shell nanoparticle was prepared is biocompatible and have potential use as drug delivery.
Clinical Effect of Soft Coagulation for Air Leak Treatment during Pulmonary Lobectomy
Authors: Takahashi Yuki , Maki Ryunosuke , Tsuruta Kodai , Tada Makoto , Arai Wataru , Shindo Yuma , Nakamura Yasuyuki , Miyajima Masahiro , Watanabe Atsushi
Published: June 2022

BackgroundSoft coagulation using the VIO soft coagulation system is used to treat minor lung air leaks during pulmonary resection in Japan. We previously reported that it has a similar effect as the air leak treatment with fibrin glue. We evaluated the efficacy of soft coagulation using the VIO soft coagulation system for lung air leakage during pulmonary resection.MethodsIntraoperative air leaks from the interlobar lung parenchyma were observed in 42 of the 283 patients who underwent video-assisted thoracoscopic surgery lobectomy between 2016 and 2018. We retrospectively reviewed these 42 patients who were treated using the VIO soft coagulation system for air leaks. We classified the air leaks in to grades using the Macchiarini scale score and evaluated the surgical outcomes of air leak treatment.Results Air leaks from the interlobar lung parenchyma having Macchiarini scale scores 1, 2, and 3 occurred in 8, 17, and 17 patients, respectively. In all the 8 patients with score 1 air leaks (100%), the air leaks could be controlled using the VIO soft coagulation system alone, and none had delayed pneumothorax requiring intervention. Of the score 2 and 3 air leaks, 52.9% and 35.3% were controlled using the VIO soft coagulation system alone, respectively.Conclusions Macchiarini scale score 1 air leaks from the interlobar lung parenchyma could be well controlled using the VIO soft coagulation system. Therefore, soft coagulation with this system may be an alternative method for treating minor air leaks during pulmonary resection surgery.
A CNN-based Lower Limb Motion Quality Evaluation Method for Home-based Stroke Rehabilitation
Authors: Ge Pin , Huang Ziyang , Tang Guoliang , Kumar Akshay , Mahmoud Seeahmed , Fang Qiang , Chen Jian
Published: June 2022

Background: With the rapid increase of stroke incidence in recent yearsworldwide, home-based rehabilitation training has become more needed,especially for remote regions or in developing countries where rehabilitationresources are scarce. Studies have demonstrated that home-based rehabilitationfor poststroke patients is essential for reducing the cost as well as for providingefficient rehabilitation. Nevertheless, home-based rehabilitation training requireseffective professional support and timely evaluation. Method: In this paper, a home-based rehabilitation quality evaluation methodfor lower limb training was proposed. The kinematic data of a patient’s lowerlimb during a set of selected training exercises was captured by a wireless bodyarea sensor network (WBASN). The data was then processed by a convolutionalneural network (CNN) based algorithm to classify the rehabilitation training typeand to evaluate the training quality. A series of kinematic features were selectedfor rehabilitation quality scoring. The experiments have been conducted using 26human participants, including 6 healthy participants and 20 stroke patients atdifferent Brunnstrom recovery stages. Results: An accuracy of 95.3% has been achieved for recognizing therehabilitation training types and a statistically significant linear positivecorrelation (R2 = 0.9962) has been obtained between the objective scores andthe Brunnstrom stages evaluated by the clinicians.
Qualitative study of process in daily life of patients with larynx/pharyngeal cancer before total laryngectomy
Authors: Kotake Kumiko , Haba Kaori , Kurita Mami , Ota Ichiro , Suzukamo Yoshimi , Kai Ichiro , Iwanaga Kazuyo , Takahashi Aya , Ishibashi Yoko , Uemura Hirokazu , Hara Yoriko , Atrata Yukiko , Kinoshita Yasuhito
Published: June 2022

Background
Many patients with larynx/pharyngeal cancer only become aware of the disease in an advanced stage due to hoarseness and a strange feeling upon swallowing. The aim of this study was to discuss what kinds of support are required for these patients by clarifying the process in their daily life prior to total laryngectomy.
Methods
A qualitative study was performed using the Modified Grounded Theory Approach in patients with larynx/pharyngeal cancer before total laryngectomy. In a semi-structured interview, patients were asked to talk freely about their health conditions, problems, and anxieties. Information on age, sex, family members living with the patient, occupation, diagnosis, staging, and subjective symptoms was also obtained in the interview.
Results
The analysis theme of “How do cancer patients live before surgery?” had a storyline centered on {surviving surgery}. The patients had a [strange feeling] caused by a sense of a lump in the throat and pain of hoarseness, as well as [unexperienced anxiety] for their first experience of hospitalization and surgery. The core category and the two categories interacted with each other and recurred. [Unexperienced anxiety] was related to [cannot imagine] daily life after hospital discharge, and transitioned to [not knowing what will happen after surgery] until the time comes. The core category of {surviving surgery} and the three categories interacted with each other and recurred. The patients also had a positive mindset of [having a purpose in life after hospital discharge] and were [looking for positives] at that time, while making a [request of support from physicians] to improve their [strange feeling]. Quality of life (QOL) prior to laryngectomy is influenced by negative mental and physical feelings caused by cancer and by anxieties about postoperative life, but with hope for social support.
Conclusions
Patients experience a “transition” before total laryngectomy, and at this turning point they try to think positively about surviving the surgery, but also feel loneliness, fear, and anxiety. These findings suggest the need for a healthcare team approach for pre- and postoperative support by staying close to a patient, even after hospital discharge.
Synthesis of plant-mediated silver nanoparticles using Veronica anagallis-aquatica extract; Optical and biological properties
Authors: Ahmed Mushtaq , Khan Aman , Mushtaq Nadia , Sher Naila , Khan Rahmat Ali
Published: June 2022

There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy, and medicine. Metallic NPs are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. The focus of the current study was the green synthesis of silver nanoparticles (AgNPs) from 1 mM silver nitrate (AgNO3) solution using an aqueous solution of Veronica anagallis-aquatica (V. anagallis-aquatica). Fixed ratio of AgNO3 and plant extract was mixed and conformation of AgNPs was done by UV visible spectroscopy; which showed a maximum absorption peak at 425 nm. The Fourier transformed spectroscopy (FTIR) spectrum shows various peaks for different functional groups bearing compounds that had played role in the bioreduction of AgNPs. Scanning electron microscopy (SEM) analysis demonstrated that AgNPs were spherical with a size of about 20 nm calculated by Nano measurer software. The average size of 11.45 nm was calculated using four major peaks 38°, 44°, 64°, and 78° of X-rays diffraction (XRD) analysis. The chemical composition of AgNPs was revealed by Energy dispersive X-ray (EDX). The atomic and weight percentages of Ag, Na, Mg, and O were 73.55, 17.91, 6.78, 1.75, and 60.58, 21.20, 8.49, and 9.74. EDX confirmed the intense sharp peak at 73.55 keV. The in vitro antioxidant, antibacterial, antifungal, antidiabetic, and TBARS were found with positive results. The current study concluded that V. anagallis-aquatica possesses the ability to synthesize AgNPs. These synthesized AgNPs showed enhanced properties compared to the V. anagallis-aquatica.
A 5-year outcome of Propranolol for the treatment of paediatric intracranial cavernoma: Case report and a review of the literature
Authors: Tiefenbach Jakov , Park Jay J , Kaliaperumal Chandrasekaran
Published: June 2022

We describe a case of a young male patient with KRIT1 driven familial cavernous malformation syndrome who developed multiple brain cavernomas, intracranial bleeding, and persistent seizures. Due to the relentless growth of cavernous malformations and recurrent intracranial bleeds, it was decided to enrol the patient in the “Propranolol for Intracranial Cavernoma” (PICC) pilot trial at our institution. Over the five-year treatment period with 20–40mg oral propranolol three times daily (TDS), we noted near-complete arrest of the growth of cavernous malformations with no further evidence of intracranial bleeding or any further seizures. The observed outcome is consistent with the extremely limited published literature on the topic; thus, this case provides important evidence that supports the use of propranolol as a prophylactic treatment for paediatric intracranial cavernomas. We strongly encourage and recommend future prospective randomized controlled trials to definitively assess and characterize the therapeutic utility of propranolol in this patient population.