bluuebunny/crossref_metadata_2025 huggingface dataset:
Radiofrecuencia térmica en alivio del dolor lumbar de origen facetario. Resultado de una serie de casos
Authors: Rodriguez Jose Fernando , Calle Alejandra , Rodriguez Rene Felipe , Arias Claudia Marcela , Caracas Lina Susana , Gonzalez Daniel Santiago , Benitez Carlos Gustavo , Rodriguez Rene Fernando
Published: April 2020

Introducción: El dolor lumbar es causa frecuente de consulta médica, responsable de 10-15% de incapacidades laborales. En Colombia es la tercera causa de consulta en servicios de urgencias, la cuarta en medicina general, la primera causa de reubicación laboral y la segunda de pensiones por invalidez, por esto la importancia de evaluar la efectividad de diferentes medidas terapéuticas, como la radiofruencia, para alivio de esta clase de dolor.Objetivos: Describir los datos clínicos, terapéuticos y evolutivos a corto plazo de la radiofrecuencia térmica de los ramos mediales en el alivio del dolor lumbar de origen facetario.Métodos: Se realizó un estudio transversal de la cohorte de pacientes con dolor lumbar facetario sometidos a radiofrecuencia térmica. Se analizaron variables demográficas y clínicas; se evaluaron en el primer control al mes y controles trimestrales, hasta un año de seguimiento. Se calificó como mejoría al procedimiento aquel paciente que con disminución del dolor <50% con respecto al basal.Resultados: El procedimiento fue efectivo en 80% de los pacientes; disminuyó progresivamente con el tiempo: 3 meses (70%), 6 (61%), 9 (44%) de mejoría y 12 (disminuyó a 25%). Como efectos adversos, un paciente refirió incremento inicial del dolor y otro sensación de disminución de la fuerza de las piernas que desapareció durante la primera semana.Conclusiones: La radiofrecuencia térmica de los ramos mediales disminuye el dolor lumbar crónico de origen facetario. La mejoría se reduce gradualmente. La baja incidencia de efectos colaterales y complicaciones de la radiofrecuencia justifican su utilización.
Caracterización clínica y bioquímica de la mujer con Síndrome de Ovario Poliquístico
Authors: Builes Carlos Alfonso , Díaz Ivonne , Castañeda Jimmy , Pérez Luis Ernesto
Published: April 2020

El Síndrome de Ovario Poliquístico (SOP) tiene una prevalencia entre 4 y 8% en la población general joven (18 a 45 años de edad). Su diagnóstico se hace con la presencia de dos de los siguientes tres criterios: oligo-anovulación, anovulación, hiperandrogenismo clínico o asintomático y morfología poliquística del ovario al ultrasonido. En el estudio del SOP se deben descartar otras causas de hiperandrogenismo como hiperplasia adrenal congénita, síndrome de Cushing y tumores productores de andrógenos. Las pacientes con SOP tienen alto riesgo de desarrollar intolerancia a los carbohidratos, diabetes y síndrome metabólico, pero no existe evidencia clínica contundente que respalde en ellas una mayor incidencia de eventos isquémicos del miocardio, ni de cáncer endometrial.
Degradação de ecossistemas marinhos: análises a partir do caso do descarte de efluentes de estações de tratamento offshore à luz dos princípios da prevenção e da precaução
Authors: Mafort Antonio Vinicius Lamblet , Ferreira Maria Inês Paes , Pinto Augusto Eduardo Miranda
Published: April 2020

Acompanhando o crescimento das atividades navais, a degradação dos oceanos surge como uma grande preocupação e motivo de alerta às ações conservacionistas desses ambientes. Os objetivos de desenvolvimento sustentável (ODS) propostos pela Organização das Nações Unidas corroboram esses fatos e ratificam a importância ambiental dos oceanos. Por meio de revisão bibliográfica, este artigo busca analisar as regulamentações acerca da suficiência de políticas públicas que preencham lacunas relativas aos descartes de efluentes sanitários de embarcações e que ratifiquem a importância de se empregarem os princípios da precaução e prevenção para que abordagens, por meio de novas regulamentações, estabeleçam parâmetros, até então inexistentes, de controle de qualidade de efluentes condizentes com as proposições de sustentabilidade ambiental abordadas nos ODS e  aplicáveis ao caso brasileiro.
A utilização de graxas em revestimentos durante a construção de poços de petróleo: análise de Fichas de Informações de Segurança de Produtos Químicos
Authors: De Almeida Samantha Souza , De Oliveira Manildo Marcião , Serejo Arthur Neves Fraga
Published: April 2020

A poluição oceânica vem ganhando cada vez mais notoriedade, tamanha a frequência da constatação de que muitas espécies aquáticas e regiões (até então preservadas) têm sido afetadas direta ou indiretamente por ações individuais e corporativas. Diante da lacuna existente nos registros relacionados à utilização de graxas em revestimentos instalados em poços de petróleo (tanto onshore quanto offshore), considerando as premissas da ecotoxicologia, este estudo destaca a fragilidade na divulgação dos parâmetros ambientais relacionados a algumas das graxas de estocagem (Kendex OCTG e Jet-LubeKorr-Guard) e descida (Lubrax Lith PM1 e Bestolife 72733) mais utilizadas no Brasil. Considerando a existência de opções que dispensam a utilização de tais produtos químicos durante todo o ciclo de vida dos produtos (tecnologia “dope-free”), o aprofundamento de estudos afins (especialmente testes de toxicidade), bem como a adoção de medidas com menor potencial poluidor nas atividades de construção de poços, são sugeridos.
Pertanggungjawaban Pidana Lembaga Konservasi Atas Eksploitasi Satwa Liar Dilindungi Dalam Peragaan Satwa
Authors: Ariagana Balma
Published: April 2020

Penelitian ini dalam penulisannya menggunakan tipe penelitian hukum doktrinal yang bersifat normatif. Pendekatan yang dipilih dalam menyusun penelitian ini adalah pendekatan peraturan perundang-undangan dan pendekatan konseptual. Bahan hukum yang digunakan adalah peraturan perundang-undangan yang relevan sebagai bahan hukum primer dan buku-buku, jurnal-jurnal, surat kabar baik cetak maupun elektronik sebagai bahan hukum sekunder. Bahan hukum tersebut dianalisis untuk menjawab dan menjelaskan rumusan masalah berupa Eksploitasi Satwa Liar Yang Dilindungi Yang Berimplikasi Tindak Pidana dan Pertanggungjawaban Pidana Terhadap Lembaga Konservasi Pelaku Eksploitasi Satwa Liar Dilindungi. Dengan menjawab rumusan masalah tersebut, diharapkan dapat menjelaskan bentuk-bentuk eksploitasi satwa liar dilindungi yang berimplikasi tindak pidana dan menjelaskan bentuk pertanggungjawaban pidana yang dapat diterapkan bagi lembaga konservasi pelaku eksploitasi satwa liar dilindungi. Satwa liar dilindungi dieksploitasi oleh lembaga konservasi dengan modus operandi berupa edukasi atraksi satwa. Dalam prakteknya, atraksi satwa tersebut ditemukan penyiksaan dan penyalahgunaan satwa. Lembaga-lembaga tersebut memanfaatkan izin konservasi yang dimilikinya untuk menyiksa satwa yang dikelolanya demi mendapatkan keuntungan, padahal satwa liar dilindungi merupakan salah satu bentuk keanekaragaman hayati yang harus dijaga kelestariannya mengingat jumlahnya yang semakin mendekati kepunahan. Penyiksaan dan penyalahgunaan satwa dalam eksploitasi tersebut mengancam kesejahteraan hewan yang dimiliki satwa liar dilindungi yang berada di pengelolaannya. Hasil penelitian menunjukan bahwa: eksploitasi satwa liar dilindungi merupakan peragaan satwa yang bertransformasi memjadi eksploitasi yang berimplikasi tindak pidana melukai satwa dilindungi dalam keadaan hidup berdasarkan UU Konservasi dan oleh karena itu Pengurus lembaga konservasi adalah pihak yang dapat dimintai pertanggungjawaban pidana atas eksploitasi satwa liar dilindungi yang berimplikasi tindak pidana berdasarkan undang-undang tersebut.
Keabsahan Permohonan Pailit Yang Diajukan Oleh Buruh Tanpa Putusan Pengadilan Hubungan Industrial
Authors: Fitasari Farlina Dwi
Published: April 2020

Kepailitan merupakan upaya kreditor untuk mendapatkan pembagian utang yang dimiliki debitor secara adil. Buruh memiliki kedudukan dalam Kepailitan, yaitu sebagai Kreditor Preferen. Hal ini diatur dalam Pasal 95 ayat (4) UU Ketenagakerjaan, bahwa upah pekerja mendapatkan pemenuhan terlebih dahulu dalam hal suatu perusahaan dinyatakan Pailit. Dengan demikian, maka pekerja dapat menjadi pemohon pailit apabila terdapat upah yang menunggak dan tidak dibayarkan oleh Pemberi Kerja (telah jatuh tempo). Pekerja tidak perlu menunggu Putusan Pengadilan Hubungan Industrial (PHI) terlebih dahulu untuk menagih upah yang tidak dibayarkan, apabila telah memenuhi syarat Kepailitan secara sederhana dan terbukti bahwa tidak terdapat sengketa Upah Buruh Terutang antara buruh dan Pemberi Kerja, maka Buruh dapat langsung mengajukan permohonan pailit kepada Pengadilan Niaga. Dengan dibuktikannya hal tersebut, maka permohonana pailit tidak bersifat prematur lagi.. Dalam penelitian ini akan membahas mengenai legal standing buruh dalam mengajukan permohonan pailit tanpa putusan PHI, dan penghitungan buruh sebagai pemohon pailit yang diwakili oleh Serikat Pekerja nya. Penulisan ini menggunakan metode penelitian hukum yuridis normatif dengan pendekatan statute approach, conceptual approach, dan case approach. Hasil dari penelitian yang dilakukan penulis yaitu buruh dapat mengajukan permohonan pailit apabila telah memenuhi syarat sederhana Kepailitan, untuk membuktikan keabsahan jumlah upah pekerja yang akan dijadikan sebagai obyek permohonan pailit, pekerja dapat menjadikan Pasal 28 Permenaker 33/2016. Dan dalam hal mengajukan permohonana pailit, buruh dihitung sebagai subyek individu, meskipun diwakili oleh Serikat Pekerja.
L’utilisation des drêches de blé en alimentation porcine
Authors: COZANNET P. , PRIMOT Y. , MÉTAYER J.P. , GADY C. , LESSIRE M. , GERAERT P.A. , LE TUTOUR L. , SKIBA F. , NOBLET J.
Published: April 2020

Parallèlement à l’accroissement rapide de la demande d’éthanol, essentiellement à base de blé en Europe, la disponibilité pour l’alimentation des animaux d’élevage des coproduits associés, tels que les drêches de blé s’est accrue. La synthèse réalisée dresse le profil moyen de la composition des drêches de blé et indique l’existence d’une forte variabilité de la composition des drêches pour certains paramètres. Ces modifications du profil moyen dépendent des spécificités de traitement du produit. La variabilité est ainsi très importante pour la teneur en parois végétales (NDF) et en amidon dont les teneurs moyennes (min-max) sont respectivement de 28 (23-33) et 4,7 (2,1-10,3)% de la MS. Les teneurs moyennes en énergie digestible de 14,2 MJ d’ED/kg MS et en phosphore digestible de 0,60% MS illustrent le potentiel des drêches pour l’alimentation des porcs. Ces valeurs varient selon les sources (12,8 à 16,0 MJ/kg pour l’ED). La teneur en lysine des protéines (0,83 à 3,0%) et la digestibilité iléale standardisée de la lysine (49 à 72%) sont les paramètres les plus variables, vraisemblablement en lien avec le procédé mis en œuvre (séchage notamment). La prise en compte des caractéristiques physiques (odeur et couleur) devrait permettre de prédire la valeur nutritionnelle et de conduire à l’inclusion de drêches à des niveaux plus importants dans l’aliment des porcs.
Modulation de la qualité de la viande de porc par l’alimentation
Authors: MOUROT J. , LEBRET B.
Published: April 2020

Parmi les facteurs d’élevage influençant la qualité de la viande chez le porc, l’alimentation joue un rôle central. Le niveau et le profil de distribution de la ration (restriction puis réalimentation) influencent la vitesse et la composition du gain de poids (protéines/lipides) aux niveaux corporel et tissulaire, et, donc, la composition de la carcasse et des tissus. La qualité sensorielle de la viande peut être ainsi modifiée, notamment via la teneur en lipides intramusculaires. L’équilibre entre les principaux nutriments, notamment les protéines et l’énergie de la ration, conditionnent également la vitesse et la nature des dépôts tissulaires, pouvant ainsi affecter la qualité sensorielle des viandes. La nutrition constitue un puissant levier pour modifier le profil lipidique des viandes porcines, la teneur en acides gras (polyinsaturés n-3 notamment), et par suite leur valeur nutritionnelle.
Elevage bio : des cahiers des charges français à la réglementation européenne
Authors: LEROUX J. , FOUCHET M. , HAEGELIN A.
Published: April 2020

L’apparition de cahiers des charges nationaux de l’élevage biologique dès les années 90 a permis un premier développement de ces élevages variable selon les productions. Le règlement européen a ensuite pris le relais en 2000, d’abord avec un complément national (le CC REPAB F) puis, à compter du 1er janvier 2009, avec un nouveau dispositif sans possibilité de subsidiarité.
Le règlement européen de l’agriculture biologique décline chaque grand principe de ce mode de production en un certain nombre de règles détaillées, et, dans certains cas, de dérogations. Ces principes sont les suivants : choix de races appropriées, naissance et élevage des animaux en bio, réduction des pollutions et du surpâturage, accès permanent à l’extérieur, densité et organisation des bâtiment permettant le bien-être, interdiction de l’attache des animaux, réduction des mutilations, méthodes de reproduction naturelles privilégiées, élevage extensif, lien sol-plante-animaux sur l’exploitation, alimentation adaptée et biologique, absence d’OGM, santé animale passant d’abord par la prévention via les conditions d’élevage, et des traitements homéopathiques et phytothérapiques, etc.
Les auteurs se sont attachés à mettre en relief les changements réglementaires récents et les enjeux techniques, économiques ou d’image qu’ils soulèvent.
En conclusion, il est souligné l’importance du travail à réaliser sur trois grands axes : les questions techniques liées à la production biologique, les verrous techniques ou économiques liés à la mise en place des filières, et l’analyse des systèmes de production dans leur globalité.
SIFAT KIMIAWI MENTEGA KETAPANG (Catappa butter) (Pengaruh Lama Sangrai Dan Lama Rendam)
Authors: Wijonarko Gunawan , Erminawati Erminawati , Handayani Isti
Published: April 2020

Biji ketapang mempunyaicita rasa yang enak dan komposisi kimia yang mirip dengan kacang tanah. Oleh karena itu biji ketapang mempunyai potensi yang sangat besar untuk dibuat menjadi produk pangan baru. Salah satu produk pangan tersebut adalah mentega ketapang (Catapa butter). Berdasarkan hal tersebut maka perlu dilakukan penelitian yang bertujuan untukmengevaluasi sifat kmiawi mentega ketapang yang dibuat pada berbagai : (1) lama penyangraian (2) lama perendaman  (3) lama penyangraian dan perendaman.Rancangan yang digunakan dalam peneltian ini adalah Rancangan Acak Lengkap. Faktor yang dicoba adalah lama penyangraian (4 ; 8 ; dan 12 menit) dan lama prendaman (0 dan 12 jam). Perlakuan disusun secara faktorial sehingga diperoleh 6 kombinasi perlakuan. Percobaan diulang 4 kali dengan 24 unit percobaan. Variabel yang diamati adalah kadar air, abu, serat kasar, lemak dan protein. Data hasil pengamatan dianalisis menggunakan Uji F. Jika terdapat perbedaan nyata antar perlakuan maka dilanjutkan dengan Uji Rentang Jamak Duncan (DMRT) untuk menentukan perlakuan terbaik. Perlakuan terbaik kemudian dibandingakn dengan selai kacang tanah non komersial. Hasil penelitian menunjukkan lama penyangraian berpengaruh nyata terhadap kadar air dan abu sedangkan perendaman berpengaruh nyata terhadap kadar air, abu dan protein. Kombinasi keduanya memberikan pengaruh yang nyata hanya terhadap kadar protein. Penyangraian 8 menit menghasilkan mentega ketapang dengan kadar air 2,8% dan abu 2,6% sedangkan perendaman 0 jam menghasilkan mentega ketapang dengan kadar air 2,5%, abu 2,7% dan protein 26,5%. Kombinasi kedua perlakuan berpengaruh terhadap kadar air, abu dan protein mentega ketapang. Dibandingkan dengan SNI selai kacang tanah, mentega ketapang mempunyai karakter kimiawi yang mirip kecuali pada kadar abu yang cenderung lebih tinggi yaitu sebesar 2,7%.  Penyangraian 8 menit dan perendaman 0 jam menghasilkan mentega ketapang dengan kadar air 2,7%, abu 2,5%, serat kasar 1,8%, lemak 50,6% dan protein 27,6%.
Study of Local Herb Potency as Rumen Modifier: Red Ginger (Zingiber Officinale Var. Rubrum) Addition Effect on In Vitro Ruminal Nutrient Digestibility
Authors: Kurniawati Asih , Yusiati Lies Mira , Widodo Widodo , Artama Wayan Tunas
Published: April 2020

Red ginger is herb that commonly used as part of traditional medicine due to its essential oil content. Some essential oil have proven as rumen fermentation modifier. Addition of red ginger in ruminant diet was studied using in vitro gas production technique to evaluate its effect on nutrient digestibility. Red ginger meal was added to meet essential oil level in fermentation medium of 0 mg/l as control, 25, 50, 75 and 100 mg/l. The diet consisted of Penisetum hybride, rice bran, wheat pollard in ratio 60:20:20 DM basis. Feed fermentation was incubated for 24 h at 39°C. At the end of incubation data of gas production volume was taken, and residual feed were collected for further nutrient analysis to calculate the digestibility of dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Variance analysis was used to analysis collected data, followed by DMRT analysis.  Addition of red ginger increase total volume of gas production at level 50 mg/L and above. DM and OM digestibility did not affected by red ginger addition whereas CP digestibility were significantly decreased start at level 50 mg/l. In contrast, CF digestibility of treatment level of 50 mg/l significantly higher than control. In conclusion, addition of red ginger correspond to level essential oil of 50 to 100 mg/l improve ruminal nutrient fermentation.
HUBUNGAN PERILAKU PERSONAL HYGIENE DAN STATUS KECACINGAN DENGAN STATUS GIZI ANAK SEKOLAH DASAR DI WILAYAH KERJA PUSKESMAS II SUMBANG
Authors: Arifin Lida , Purnamasari Dyah Umiyarni
Published: April 2020

Background : Nutrition is one kind of important factors that build qualified human resources. A child being underweight due to two important things, they are food intake and illness. One type of infection that is often found in children that worm infection. The infection is often found in developing countries with lack of personal hygiene. The purpose of this research is to determine relation between personal hygiene and status worm infection with nutritional status among primary children.

 

Methods : This study design was observational analytic study with cross sectional approach. The sample were 51 children fourth grade 01 Banjarsari Kulon and 02 Banjarsari Wetan Primary School.

 

Results : Respondents with normal nutritional status 72%. Respondents with good behavior personal hygiene 59%. Respondents with positive helminthiasis status 53%. Relation between personal hygiene and nutritional status was examined by chi square (p value 1.000 > 0.05). Relation between helminthiasis status and nutritional status p value 0.425. Relation between personal hygiene and helminthiasis status p value 0.625.

 

Conclusion : There is no relation between the behavior of personal hygiene and nutritional status of school children in Puskesmas II Sumbang. There is no relation between helminthiasis status and nutritional status of school children in Puskesmas II Sumbang. There is no relation between personal hygiene and helminthiasis status.
SUBSTITUSI TEPUNG AMPAS KELAPA DALAM PEMBUATAN BROWNIES KUKUS TERHADAP SIFAT ORGANOLEPTIK DAN NILAI GIZI
Authors: Septiani Septiani , Hiyanah Nur
Published: April 2020

The utilization of coconut pulp flour in the food product making is still very limited. This research to analyze brownies that are distuited coconut pulp flour against nutritional value. This research is an experimental research with level of substitution of  wheat flour with coconut pulp flour as  treatment. There are 3 treatments, that is F1 (55% wheat flour and 45% coconut pulp flour), F2 (45% wheat flour and 55% coconut pulp flour) ad F3 (35% wheat flour and 65% coconut pulp flour). Formula results selected from the organoleptic  test who is F1 with comparison 55:45 continued with result proximate test that obtained water rate, ash rate, fat, protein, carbohydrate and fiber in way continue that 23,88%, 1,91%, 7,39%, 5,78%, 61,04 and 1,82%. The conclusion of this research is that have differences (p <0.05) between the formula result to water rate, ash rate, protein, carbohydrate and fiber.
The Effect of Addition of Ginger Extract and Kencur Extract on the Physicochemical Properties of Instant Temulawak and the Sensory Properties of the Beverage
Authors: Septiana Aisyah Tri
Published: April 2020

AbstrACT

Temulawak has health benefits, but it is sensationally less favored because it is bitter and has an unpleasant odor, so that in its processing often other spices such as ginger and kencur are added. Temulawak can be processed into temulawak instant drink. This study aims to determine the effect of the concentration of ginger extract (0%, 15%, 30%) and kencur (0%, 15%, 30%) on the physicochemical properties of temulawak instant and sensory brewed drinks. Physicochemical measurements were carried out by analyzing the water content and density of cages of temulawak instant drink. Sensory analysis included color, aroma, spicy taste, sweetness, flavor and likeness. The results showed that the addition of ginger and kencur extract tended to increase water content. The addition of ginger extract and kencur up to 15% increase the flavor and preference of ginger drink. The best combination of the addition of ginger extract and kencur extract was obtained in the treatment of adding ginger extract 15% and the concentration of galingale extract 15% with physicochemical properties of powder drink: water content of 5.20% bb and kamba density of 0.608 g / ml. color 3.24 (brownish yellow); 2.68 spicy flavor (approaching somewhat spicy); sweetness level 3,4 (somewhat sweet); flavor 3.51 (close to tasty); fondness 3.43 (few likes).
COVID-19 Pandemic and the Digital Revolution in Academia and Higher Education
Authors: Strielkowski Wadim
Published: April 2020

COVID-19 pandemic instigated a digital revolution in academia and higher education. Social distancing, months-long quarantine, as economic shutdown will help the majority of people working in academia and higher education not only to complete their personal transition to the fully functional and operational online tuition, but also to understand that online defences, online entrance and final exams, as well as online academic jobs are as effective and meaningful as those conducted “in real life”. Due to the crisis induced by the coronavirus epidemic, innovations in academia and higher education that would have normally taken several years due to the various contradictory administrative regulations are now introduced promptly in a matter of days. This is a clear example of the Schumpeterian ‘creative destruction’ in making that will forever change the status quo in academia and higher education.
Solving Mixed Volterra - Fredholm Integral Equation (MVFIE) by Designing Neural Network
Authors: Et al. Al-Saif
Published: April 2020

In this paper, we focus on designing feed forward neural network (FFNN) for solving Mixed Volterra – Fredholm Integral Equations (MVFIEs) of second kind in 2–dimensions. in our method, we present a multi – layers model consisting of a hidden layer which has five hidden units (neurons) and one linear output unit. Transfer function (Log – sigmoid) and training algorithm (Levenberg – Marquardt) are used as a sigmoid activation of each unit. A comparison between the results of numerical experiment and the analytic solution of some examples has been carried out in order to justify the efficiency and the accuracy of our method.
A Tangent-Line Approach for Effective Density Used in the Ideal Mixing Rule: Part II—Evaluation of Mixing Characteristics of Oil/Gas Systems and Application Criteria
Authors: Chen Zehua , Yang Daoyong (Tony)
Published: April 2020

Although in Part I of this study (Chen and Yang 2020) we developed a tangent-line approach for effective density that is more general, robust, and flexible than the methods proposed by Saryazdi (2012) and Saryazdi et al. (2013), its application is only limited to heavy-oil/bitumen-associated mixtures [i.e., specifically, it has only been applied to bitumen-rich liquid phase (denoted as L2)]. As indicated in Part I, the density of nitrogen (N2)/hydrocarbon mixtures cannot be accurately predicted by using the ideal mixing rule (IM) with either real density or effective density. Not only do we need to explain and evaluate the observed deviations and patterns, but also the density prediction of solvent/Fraction 1 systems [i.e., Fraction 1 of the Athabasca bitumen, which has a molecular weight (MW) of 268.8 g/mol, as reported in Azinfar et al. (2018a, 2018b, 2018c)] needs to be improved for practical use. In this study, we evaluate the mixing characteristics of different molecules in a mixture using the tangent-line approach. By evaluating and comparing performances of the IM with effective density (IM-E) and the IM with real density (IM-R), the observed patterns and deviations together with those calculated from the Westman equation indicate that the oil/gas molecules somewhat behave like solid particles in mixing. Accordingly, we further modify the effective density used in the IM to bridge the gap between the IM-E and the IM-R. The database has been extended to light-oil/gas systems such as black oils, volatile oils, gas condensates, carbon dioxide (CO2) miscible fluids, sour gases, and wet/dry gases. The IM with modified effective density (IM-ME) has also been applied to solvent/Fraction 1 systems and the C2 or C3 or n-C4-extraction L1 phase (bitumen-related mixtures) with better accuracy. Also, we develop new criteria for the uses of the IM-E, IM-ME, and IM-R that can cover the density predictions for almost all types of oil/gas systems in the petroleum industry with high accuracy. The performances of the IM are thoroughly evaluated and compared with the volume-translated (VT) Peng-Robinson equation of state (EOS) (VT PR EOS), from which the deviations provide new insights for accurately quantifying the mixture density in a more robust and reliable manner.
Successful pregnancy after complete resection of leiomyomatosis peritonealis disseminate without recurrence:A case report with next generation sequencing analysis and literature review
Authors: Bu Hualei , Jin Chengjuan , Fang Yan , Ma Yana , Wang Xiao , Chen Jingying , Chen Lijun
Published: April 2020

Background: Peritoneal leiomyomatosis disseminate (LPD) is a rare disease characterized by widespread dissemination of leiomyomas nodules throughout the peritoneal and omental surfaces. Reports of pregnancy with LPD are even rarer. Therefore, there is no clear consensus on the treatment of LPD on pregnancy, and the pathogenesis is still unclear. Case presentation：We reported a case of LPD patient who developed during pregnancy. The patient underwent cesarean section at 32 weeks of gestation while removing all visible tumors, and no LPD lesions were seen in the subsequent cesarean section at full-term. NGS of LPD lesions detected 4 mutations with focal high-level amplifications of CDK4 (Cyclin-dependent kinases 4), NBN (Nibrin), DAXX (Death domain associated protein) and MYC (Myelocytomatosis oncogene). Immunohistochemistry staining analysis among benign leiomyoma, LPD and leiomyosarcoma verified that LPD was an unusual intermediate between benign and malignant uterine smooth muscle tumors. Besides, LPD is a hormonal-dependent leiomyoma. After detailed literature search, we summarized the detailed clinical features and follow-up information of patients with LPD during pregnancy.Conclusions: This is the first reported LPD case of successful term pregnancy without recurrence, following resection of all visible lesions in prior pregnancy. LPD is an unusual intermediate between benign and malignant uterine smooth muscle tumors.
Kethoxal-assisted single-stranded DNA sequencing (KAS-seq) for capturing transcription dynamics and enhancer activity
Authors: He Chuan , Wu Tong , Lyu Ruitu
Published: April 2020

We describe a rapid and sensitive labeling of single-stranded DNA for sequencing (KAS-seq), based on a kethoxal-guanine reaction. KAS-seq procedure involves N
3
-kethoxal labeling, DNA isolation, biotinylation, fragmentation and enrichment, library preparation and sequencing. The whole process can be finished in a day. KAS-seq enables rapid measurement of the dynamics of transcriptionally-engaged Pol II, transcribing enhancers, Pol I and Pol III activities, and non-canonical DNA structures involving ssDNA simultaneously
in situ
, by using as few as 1,000 cells or mice tissues. The robust and tissue-friendly nature coupled with low input material requirement make KAS-seq a method that can be broadly applied. This step-by-step protocol is related to the publication “Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity
in situ
”

in
Nature Methods
.
A protocol for evaluating a multi-level implementation theory to scale up obstetric triage in referral hospitals in Ghana
Authors: Williams Caitlin Rain , Bogdewic Stephanie , Owen Medge , Srofe Emmanuel K. , Ramaswamy Rohit
Published: April 2020

Background: Ghana significantly reduced maternal and newborn mortality between 1990 and 2015, largely through efforts focused on improving access to care. Yet achieving further progress requires improving the quality and timeliness of care. Beginning in 2013, Ghana Health Service and Kybele, a US-based non-governmental organization, developed an innovative obstetric triage system to help midwives assess, diagnosis, and determine appropriate care plans more quickly and accurately. In 2019, efforts began to scale this successful intervention into six additional hospitals. This protocol describes the theory-based implementation approach guiding scale-up and presents the proposed mixed-methods evaluation plan. Methods: An implementation theory was developed to describe how complementary implementation strategies would be bundled into a multi-level implementation approach. Drawing on the Interactive Systems Framework and Evidenced Based System for Implementation Support, the proposed implementation approach is designed to help individual facilities develop implementation capacity and also build a learning network across facilities to support the implementation of evidence-based interventions.A convergent design mixed methods approach will be used to evaluate implementation with relevant data drawn from tailored assessments, routinely collected process and quality monitoring data, textual analysis of relevant documents and WhatsApp group messages, and key informant interviews. Implementation outcomes of interest are acceptability, adoption, and sustainability.Discussion: The past decade has seen a rapid growth in the development of frameworks, models, and theories of implementation, yet there remains little guidance on how to use these to operationalize implementation practice. This study proposes one method for using implementation theory, paired with other kinds of mid-level and program theory, to guide the replication and evaluation of a clinical intervention in a complex, real-world setting. The results of this study should help to provide evidence of how implementation theory can be used to help close the “know-do” gap.
WITHDRAWN: Rumen fermentation kinetics as impacted by red amaranth (Amaranthus cruentus, L) leaf powder using in vitro gas production technique
Authors: Ampapon Thiwakorn , Viennasay Bounnaxay , Wanapat Metha
Published: April 2020

Background
A need for research searching for alternative rumen enhancers warrants immediate attention. The in vitro fermentation experiment was conducted using factorial arrangement of two factors of roughage to concentrate and seven level of red amaranth leaf powder percentage of total substrate in a Completely randomized design (CRD). Two factors, namely Factor A was two ratio of roughage (R) to concentrate (C) at 60:40 and 40:60 and Factor B was level of red amaranth (Amaranthus cruentus, L) leaf powder (RALP) supplementation at 0, 2, 4, 6, 8, 10, and 12% of total dietary substrate.
Results
Red amaranth leaf powder (RALP) contained phytonutrients both condensed tannins and saponins in addition with high macro minerals (Ca, K, and Mg). This experiment revealed innovations of the RALP supplementation by enhancing rumen propionate (C3) production, reducing acetate (C2) to (C3) ratio, reducing protozoal population and mitigating methane (CH4) production. Furthermore, rumen dry matter degradation percentages were remarkably enhanced (P < 0.001) by increasing RALP supplementation.
Conclusion
Plants rich in phytonutrients and minerals such as red amaranth leaf powder (RALP) have a vital and promising role in modulating rumen fermentation, mitigating methane production, as well as increasing substrate degradability.
Antidyskinetic Treatment with the mGluR5 Antagonist MPEP Affects CaMKII/CREB/BDNF Molecular Pathways in the Parkinsonian Striatum
Authors: Huang Yixian , Zhang Qilin , zhan zhoubing , Shu Haiyang , Zhao Junyan , Zhen Tili , Luo Weifeng
Published: April 2020

Background

L-DOPA is still the gold-standard drug for the treatment of Parkinson's disease (PD). However, the long-term therapy often causes L-DOPA-induced dyskinesia (LID). Metabotropic glutamate receptor type 5 (mGluR5) is abundant in the basal ganglia, and its antagonists is thought to alleviates LID, but the underlying mechanisms have remained unclear.
Methods

We used 6-hydroxydopamine-lesioned rats to create PD rat model, PD rats were daily treated with L-DOPA alone or with MPEP 30 min before L-DOPA for 3 weeks, and at least 21 days of L-DOPA was administrated followed with microinjection of saline, CaMKII antagonist KN-93, anti-CREB, or anti-BDNF into the lesioned striatum of all the PD rats. The behavioral evaluation of abnormal involuntary movements(AIM) and rotational behavior tests were performed on the 2, 9, 11, 18, 21 and 23 days after drug application, and to tested the protein level of mGluR5, CaMKII, CREB and BDNF by western blot.
Results

Our results showed that MPEP cotreatment attenuates the abnormal involuntary movements, reversed the reduction of rotational response duration, and reduced overexpression of striatal mGluR5 and CaMKII/CREB/BDNF in the LID rats. Furthermore, we found that the CaMKII inhibitor KN-93, anti-CREB and anti-BDNF intrastriatal injection partly attenuates LID; KN-93 downregulated the striatal p-CaMKII, p-CREB and BDNF expression of the lesioned side, but the striatal mGluR5 expression without inhibition; anti-CREB downregulated the striatal p-CREB and BDNF expression of the lesioned side, but the striatal mGluR5 and p-CaMKII expression without inhibition; anti-BDNF downregulated the striatal BDNF expression of the lesioned side, but the mGluR5, CaMKII and CREB protein without inhibition.
Conclusions

These fundings suggested that mGluR5 specific antagonist MPEP attenuates L-DOPA-induced dyskinesia through affects CaMKII/CREB/BDNF molecular pathways in the striatum in this LID rat model.
Effect combined learning on oral health self-efficacy and self-care behaviors of students: a randomized controlled trial
Authors: Hashemi Zahra Sadat , khorsandi Mahboobeh , Shamsi Mohsen , Moradzadeh Rahmatollah
Published: April 2020

Background: In order to prevent oral diseases, the use of appropriate educational methods at childhood is one of the most important determinants of the public health. Therefore, the aim of this study was to investigate the effect of training through animations and games on oral health self-efficacy and self-care behaviors in students aged 6-12 years old. Methods: In this interventional study, 82 students were selected using cluster random sampling (38 subjects in the case group and 44 in the control group). The case group received 4 sessions of blended learning per week including animations and games while the control group received routine school education. Data were collected in six domains, including demographics, self-care, knowledge, attitude, behavior and self-efficacy before and 5 months after the intervention using a questionnaire. SPSS version 20 was used for data analysis. Results: Five months after the intervention, the mean score of self-care increased from 3.8 to 4.8 of 5, the mean score of self-efficacy increased from 36.8 to 48.9 of 70, and the mean score of behavior increased from 17.07 to 18.29 of 32, indicating significant changes (p < 0.05). There were no significant changes in these variables in the control group (p >0.05) Conclusion: Use of combined methods for oral health self-care education has positive effects on the students' performance and self-efficacy. IRCT registration number: This trial has been registered at IRCT. IRCT2017042133565N1 Registration date: 2017-05-17 https://en.irct.ir/trial/25851
Exploration on the clinical effect of different autotransfusion methods on patients with femoral shaft fracture surgery
Authors: Wang Huan , Yin Dan , Wang Jin-huo , Li Zhen-zhou , Xu Jia-Ming , Zhang Zi-Wei , Wang Xiao-Xiao , Kang Yi-Qun , Guo Jian-rong
Published: April 2020

BackgroundTo explore the clinical effect of predeposit, salvage and hemodilution autotransfusion on patients with femoral shaft fractdure (FSF) surgery.MethodsSelected FSF patients were randomly divided into 3 groups: intraoperative blood salvage autotransfusion group, preoperative hemodilution autohemotransfusion group and predeposit autotransfusion. After the operation for 5 days, body temperature, heart rate, blood platelet (PLT) and hemoglobin (Hb) of patients were determined; the concentrations of EPO and G-MSF in the 3 groups were calculated by ELISA assay; the content of CD14 + monocytes were calculated by FCM assay. The growth time and condition of the patient’s callus were determined at the 30th, 45th and 60th days after operation. Cox regression analysis was used to analyze the correlation between EP0, G-MSF, CD14 + mononuclear content, callus growth and autotransfusion methods.ResultsThere were no statistically significant differences in body temperature and heart rate between the 3 groups (P > 0.05). PLT and Hb in Predeposit group were markedly increased compared to that in Salvage group and Hemodilution group. The concentrations of EPO and G-MSF in Predeposit group were markedly increased compared to that in Salvage group and Hemodilution group. The content of CD14 + monocytes in Predeposit group was significantly higher compared to that in Salvage group and Hemodilution group. Predeposit autotransfusion promotes callus growth more quickly. The concentration of EP0 and G-MSF were positively correlated with callus growth in the three groups, and the content of CD14 + monocytes was not correlated in any of the three groups.ConclusionPredeposit autotransfusion promoted the recovery of FSF patients after operation more quickly compared to salvage autotransfusion and hemodilution autotransfusion.# These authors contributed equally to this work.
A study of the correlation between chalazion and intestinal flora in chinese children
Authors: Cui Yuanyue , Song Lei , Li Xie , Qiu Ting , Jin Jing , Zhao Keke , Zhu Xiaowei , Qu Jifang , Cao Qing , Jiang Bo
Published: April 2020

BackgroundChalazion is a chronic inflammatory granuloma of the meibomian gland formed on the basis of the obstruction of the meibomian gland drainage duct and the retention of secretions. It is one of the most common clinically eye diseases in children. Chronic inflammation of the meibomian glands is responsible for this disease, and the gut flora is thought to be involved in the inflammatory process. In this study, we investigated the relationship between intestinal microbial composition and children's chalazion.MethodsFecal samples were collected from 21 children with chalazion and 26 healthy children. DNA was extracted from fecal stool samples and 16S rRNA sequences in the gut flora were detected by using second second-generation sequencing technology. The results were used to compare the composition of the microbiome between patients and healthy controls.ResultsAccording to Alpha Diversity and Beta Diversity analysis, we found that there was no significant difference in bacteria diversity and relative abundance between the two groups. We compared the flora of the control group and the diseased group through Lefse analysis, and screened out 11 different species. Based on the absolute abundance of species, 43 different species were selected. Anosim analysis and metastats analysis were used to compare the flora of the control group and the diseased group again. At the species level, we found that gut_metagenome and human_gut_metagenome are the common differences in species levels obtained from the above analysis. Finally, corrplot correlation analysis was performed, suggesting that gut_metagenome has a great correlation with the number, ulceration, and recurrence of chalazion in children.ConclusionsThere was no significant difference in the diversity index and relative abundance of flora in children with chalazion compared with healthy children, but there were significant differences in some bacterial species. The gut_metagenome strains identified in this study were significantly related to the growth, ulceration, and relapse of children with chalazion. It is suggested that gut_metagenome may be a microbiological indicator which is independent of clinicopathologic factors but associated with chalazion disease .* These authors have contributed equally to this work.
Characteristics of acute pancreatitis with diabetic ketoacidosis and analysis of factors related to the length of hospital stay
Authors: Dong Fengliang , He Xiaoyan , Yang Qunying , Lou Jiangming , Wang Bei
Published: April 2020

Obejective 11% of adults with diabetic ketoacidosis (DKA) also suffer from AP. The obejective of this research was to study the characteristics of AP with DKA, the factors related to the length of hospital stay, and to explore indicators for the severity of AP.Methods A retrospective analysis of 173 AP with DKA cases treated in our hospital from July 2011 to December 2020 was performed. We analyzed the gender, age, systemic inflammatory response syndrome(SIRS), pleural effusion, blood urea nitrogen（BUN）, bedside index for the severity in acute pancreatitis（BISAP）score, white blood cell（WBC）, aspartate transaminase （AST）, lactate dehydrogenase（LDH）, blood glucose, Ranson score, partial pressure of carbon dioxide （PaCO 2 ）,triglyceride, modified CT severity index （MCTSI）, potential of hydrogen potential of hydrogen （PH）, amylase, glycated hemoglobin, C-reactive protein（CRP）, creatinine（Cr）, blood calcium, procalcitonin （PCT）, the length of stay.Result Blood calcium, LDH, BUN, pleural effusion, MCTSI, Ranson score and BISAP score were related to the length of stay (P <0.05). WBC, AST, blood glucose, PaCO 2 , triglyceride, PH, glycated hemoglobin, CRP, Cr , PCT were not related to the length of stay (P> 0.05).Conclusion The current Atlanta classification is not suitable for evaluating the severity of AP with DKA. Serum calcium, LDH, BUN, pleural effusion, and MCTSI are suitable for evaluating the length of stay and severity of AP with DKA.
RNA sequencing analysis reveals differential gene expression of CXCL2 in ACPA-positive and ACPA-negative rheumatoid arthritis
Authors: Sun Lin , Wang Xinyu , He Ning , An Zhuo , Yu Ruohan , Li Yanming , Li Yongjun , Liu Xiangyuan , Fang Xiangdong , Zhao Jinxia
Published: April 2020

Background. Anti-citrullinated protein/peptide antibodies (ACPA) play important roles in the pathogenesis of rheumatoid arthritis (RA), and are associated with RA severity. It has been suggested that ACPA-positive (ACPA+) and ACPA-negative (ACPA-) RA are different disease subsets with distinct differences in genetic variation and clinical outcomes. The aims of the present study were to compare gene expression profiles in ACPA + and ACPA- RA and identify novel candidate gene signatures that might serve as therapeutic targets. Methods. Comprehensive transcriptome analysis of peripheral blood mononuclear cells (PBMCs) from ACPA + and ACPA- RA patients, and healthy controls was performed via RNA sequencing. Genes with significantly different expressions were analyzed by cluster analysis, Gene Ontology analysis and Ingenuity Pathway analysis. A validation cohort was used to further investigate differentially expressed genes via real-time PCR and enzyme-linked immunosorbent assay. Spearman's correlation test was used to evaluate the correlation of differentially expressed genes and the clinical and laboratory data of the patients. The role of differentially expressed genes in osteoclastogenesis was further investigated. Results. There were significant differences in the expression levels of both genes and gene isoforms between ACPA + and ACPA- RA samples. Expression of C-X-C motif chemokine ligand 2 (CXCL2) was significantly increased in ACPA + RA patients than in ACPA- RA patients and healthy controls. Validation of candidate genes expression showed that CXCL2 levels in PBMCs and serum were higher in ACPA + RA patients than in ACPA- RA patients and healthy controls. CXCL2 promoted the migration of CD14 + monocytes and increased osteoclast differentiation in RA patients. RAW264.7 macrophages were used to investigate specific mechanisms, and the results suggested that CXCL2 stimulated osteoclastogenesis via ERK MAPK and NFκB pathways. Conclusion. Novel pathways associated with ACPA + RA were identified via RNA sequencing, and CXCL2 was highly expressed in ACPA + RA than in ACPA- RA. These results reveal a previously unreported role of CXCL2 during osteoclastogenesis in RA, and suggest that the blockade of CXCL2 might be a novel strategy for the treatment of RA.
World Health Organization Grading Classification for Pancreatic Neuroendocrine Neoplasms: A Comprehensive Analysis from a Large Chinese Institution
Authors: Yang Min , Zeng Lin , Ke Neng-wen , Tan Chun-lu , Tian Bo-le , Liu Xu-bao , Xiang Bo , Zhang Yi
Published: April 2020

Background Pancreatic neuroendocrine neoplasms (p-NENs) are a group of highly heterogeneous tumors with distinct clinicopathological features and long-term prognosis. In 2017, in order to better stratify patients into prognostic groups and predicting their outcomes, World Health Organization (WHO) officially updated its grading system for p-NENs which distinguished these neoplasms among Grading 1 (G1) pancreatic neuroendocrine tumors (p-NETs), G2 p-NETs, G3 p-NETs and G3 pancreatic neuroendocrine carcinomas (p-NECs). However, this new grading classification for p-NENs has not yet been rigorously validated.Methods Data of patients who were surgically treated and histopathologically diagnosed as p-NENs at West China Hospital of Sichuan University from January 2002 to December 2018 were retrospectively collected and analyzed according the novel WHO 2017 grading classification.Results We eventually enrolled 480 eligible patients with p-NENs in our present study, in which 150 patients with WHO 2017 G1 p-NETs, 158 with G2 p-NETs, 64 with G3 p-NETs and 108 with G3 p-NECs were identified. The estimated 5-year overall survival for patients with G1 p-NETs, G2 p-NETs, G3 p-NETs and G3 p-NECs was 75.8%, 58.4%, 35.1% and 11.1%, with a median survival time of 85.3mons, 67.4mons, 51.3mons and 26.8mons, respectively. Patients with G2 p-NETs present notably worse survival than those with G1 p-NETs (P = 0.03). Survival of G3 p-NETs were significantly worse than that of G1 p-NETs or G2 p-NETs (P < 0.001, P = 0.023, respectively), as well as that when comparing G3 p-NECs with G1 p-NETs or G2 p-NETs (P < 0.001, P < 0.001, respectively). Patients with G3 p-NECs showed statistically shorter survival than those with G3 p-NETs (P < 0.001). Both WHO 2017 and 2010 grading criteria could be independent predictor for the OS of p-NENs (P = 0.016, P = 0.022; respectively). The 95% confidence intervals of WHO 2017 grading classification (0.983–9.454) was slightly smaller than that of WHO 2010 criteria (0.201–13.374), indicating a relatively more accurate predicting ability for the prognosis of p-NENs.Conclusion The WHO 2017 grading classification for p-NENs could successfully allocate patients into four groups with distinct clinical features and significant survival differences, which might be superior to the WHO 2010 criteria for its better prognostic stratification and more accurate predicting ability.