bluuebunny/crossref_metadata_2025 huggingface dataset:
Penile Injuries in Wild and Domestic Pigs
Authors: Weiler Ulrike , Isernhagen Marie , Stefanski Volker , Ritzmann Mathias , Kress Kevin , Hein Charlotte , Zöls Susanne
Published: March 2016

In boars, sexually motivated mounting can not only cause problems such as lameness, but penile injuries are also reported. The relevance of penis biting in boars is discussed controversially, but reliable data is missing. In the present study, boars (n = 385) and barrows (n = 85) from experimental farms were therefore evaluated for scars, fresh wounds and severe injuries of the penis. Similarly, 321 boars from 11 farms specializing in pork production with boars, and 15 sexually mature wild boars from the hunting season of 2015/16 were included in the study. In domestic boars, a high incidence of penile injuries was obvious (76.6%–91.3% of animals with scars and/or wounds at experimental farms, 64.0%–94.9% at commercial farms). The number of boars with severe injuries was in a similar range in both groups (5.2% vs. 9.3%). At commercial farms, the number of scars but not that of fresh wounds increased per animal with age by 0.3 per week. Moreover, raising boars in mixed groups led to about a 1.5 times higher number of scars than in single-sex groups. In wild boars, a considerable proportion of animals (40%) revealed penile injuries, which were even severe in three animals. We therefore conclude that penis biting is a highly relevant and severe welfare problem in the male pig population, but this phenomenon is not limited to intensive production systems.
Prospects for Foamy Viral Vector Anti-HIV Gene Therapy
Authors: Nalla Arun , Trobridge Grant
Published: March 2016

Stem cell gene therapy approaches for Human Immunodeficiency Virus (HIV) infection have been explored in clinical trials and several anti-HIV genes delivered by retroviral vectors were shown to block HIV replication. However, gammaretroviral and lentiviral based retroviral vectors have limitations for delivery of anti-HIV genes into hematopoietic stem cells (HSC). Foamy virus vectors have several advantages including efficient delivery of transgenes into HSC in large animal models, and a potentially safer integration profile. This review focuses on novel anti-HIV transgenes and the potential of foamy virus vectors for HSC gene therapy of HIV.
Synthesis of Diethyl Carbonate from Carbon Dioxide, Propylene Oxide and Ethanol over KNO3-CeO2 and KBr-KNO3-CeO2 Catalysts
Authors: Wang Yanlou , Jia Dongdong , Zhu Zhen , Sun Yongyue
Published: March 2016

One-pot syntheses of diethyl carbonate (DEC) from CO2, propylene oxide and ethanol were carried out using different solid catalysts. The supercritical CO2 extraction method was used to separate the liquid products and reactants from the catalysts after reaction. The KNO3-CeO2 and KBr-KNO3-CeO2 were found to be active for the reaction after calcinations. The catalyst was also reusable. The thermodynamic properties of the reaction were also evaluated. The effects of various conditions, such as reaction time, amount of catalysts, molar ratio of the reactants, the composition and calcination temperature of the catalysts on the conversion and yields, were investigated, and the yield of DEC was about 13.0% with a selectivity of 38.5% over KBr-KNO3-CeO2. The yield of DEC was improved about 10-fold by using KBr-KNO3-CeO2 catalyst compared to CeO2.
Noisiness of the Surfaces on Low-Speed Roads
Authors: Gardziejczyk Wladyslaw , Gierasimiuk Pawel , Motylewicz Marek
Published: March 2016

Traffic noise is a particular threat to the environment in the vicinity of roads. The level of the noise is influenced by traffic density and traffic composition, as well as vehicle speed and the type of surface. The article presents the results of studies on tire/road noise from passing vehicles at a speed of 40–80 kph, carried out by using the statistical pass-by method (SPB), on seven surfaces with different characteristics. It has been shown that increasing the speed from 40 kph to 50 kph contributes to the increase in the maximum A-weighted sound pressure level by about 3 dB, regardless of the type of surface. For larger differences in speed (30 kph–40 kph) increase in noise levels reaches values about 10 dB. In the case of higher speeds, this increase is slightly lower. In this article, special attention is paid to the noisiness from surfaces made of porous asphalt concrete (PAC), BBTM (thin asphalt layer), and stone mastic asphalt (SMA) with a maximum aggregate size of 8 mm and 5 mm. It has also been proved that surfaces of porous asphalt concrete, within two years after the commissioning, significantly contribute to a reduction of the maximum level of noise in the streets and roads with lower speed of passing cars. Reduction of the maximum A-weighted sound pressure level of a statistical car traveling at 60 kph reaches values of up to about 6 dB, as compared with the SMA11. Along with the exploitation of the road, air voids in the low-noise surface becomes clogged and acoustic properties of the road decrease to a level similar to standard asphalt.
The Adhesive Properties of Coherent and Semicoherent NiAl/V Interfaces Within the Peierls-Nabarro Model
Authors: Linghu Yaoyao , Wu Xiaozhi , Wang Rui , Li Weiguo , Liu Qing
Published: March 2016

The work of adhesion and the interface energy of NiAl/V coherent interface systems have been investigated using first-principles methods. The adhesion of the Ni-terminated interface is larger than the Al-terminated interface. The difference in charge density and the density of states show that the Ni-terminated interface is dominated by metallic bonds, and the Al-terminated interface is dominated by metallic and covalent bonds. To account for the effects of misfit dislocations on the semicoherent interfaces, the Peierls–Nabarro model combined with generalized stacking fault energy is employed to determine the interface energy. It is found that misfit dislocations can reduce the adhesion of the interface, and the reduction increases with the maximum of the restoring force.
Introduction to Supersymmetric Theory of Stochastics
Authors: Ovchinnikov Igor
Published: March 2016

Many natural and engineered dynamical systems, including all living objects, exhibit signatures of what can be called spontaneous dynamical long-range order (DLRO). This order’s omnipresence has long been recognized by the scientific community, as evidenced by a myriad of related concepts, theoretical and phenomenological frameworks, and experimental phenomena such as turbulence, 1/f noise, dynamical complexity, chaos and the butterfly effect, the Richter scale for earthquakes and the scale-free statistics of other sudden processes, self-organization and pattern formation, self-organized criticality, etc. Although several successful approaches to various realizations of DLRO have been established, the universal theoretical understanding of this phenomenon remained elusive. The possibility of constructing a unified theory of DLRO has emerged recently within the approximation-free supersymmetric theory of stochastics (STS). There, DLRO is the spontaneous breakdown of the topological or de Rahm supersymmetry that all stochastic differential equations (SDEs) possess. This theory may be interesting to researchers with very different backgrounds because the ubiquitous DLRO is a truly interdisciplinary entity. The STS is also an interdisciplinary construction. This theory is based on dynamical systems theory, cohomological field theories, the theory of pseudo-Hermitian operators, and the conventional theory of SDEs. Reviewing the literature on all these mathematical disciplines can be time consuming. As such, a concise and self-contained introduction to the STS, the goal of this paper, may be useful.
On Variable Reverse Power Flow-Part II: An Electricity Market Model Considering Wind Station Size and Location
Authors: Gabash Aouss , Li Pu
Published: March 2016

This is the second part of a companion paper on variable reverse power flow (VRPF) in active distribution networks (ADNs) with wind stations (WSs). Here, we propose an electricity market model considering agreements between the operator of a medium-voltage active distribution network (MV-ADN) and the operator of a high-voltage transmission network (HV-TN) under different scenarios. The proposed model takes, simultaneously, active and reactive energy prices into consideration. The results from applying this model on a real MV-ADN reveal many interesting facts. For instance, we demonstrate that the reactive power capability of WSs will be never utilized during days with zero wind power and varying limits on power factors (PFs). In contrast, more than 10% of the costs of active energy losses, 15% of the costs of reactive energy losses, and 100% of the costs of reactive energy imported from the HV-TN, respectively, can be reduced if WSs are operated as capacitor banks with no limits on PFs. It is also found that allocating WSs near possible exporting points at the HV-TN can significantly reduce wind power curtailments if the operator of the HV-TN accepts unlimited amount of reverse energy from the MV-ADN. Furthermore, the relationships between the size of WSs, VRPF and demand level are also uncovered based on active-reactive optimal power flow (A-R-OPF).
Synergistic Optimization of Thermoelectric Performance in P-Type Bi0.48Sb1.52Te3/Graphene Composite
Authors: Xie Dewen , Xu Jingtao , Liu Guoqiang , Liu Zhu , Shao Hezhu , Tan Xiaojian , Jiang Jun , Jiang Haochuan
Published: March 2016

We report the synergistic optimization of the thermoelectric properties in p-type Bi0.48Sb1.52Te3 by the additional graphene. Highly dense Bi0.48Sb1.52Te3 + graphene (x wt%, x = 0, 0.05, 0.1 and 0.15) composites have been synthesized by zone-melting followed by spark plasma sintering. With the help of scanning electron microscopy, the graphene has been clearly observed at the edge of the grain in the composites. Due to the additional graphene, the composites show an improved power factor of 4.8 × 10−3 Wm−1K−2 with modified carrier concentration and suppressed lattice thermal conductivity. Consequently, synergistic optimization in electrical and lattice properties by additional graphene leads to a great improvement in the figure of merit ZT (1.25 at 320 K).
Development of an ICE-Based Micro-CHP System Based on a Stirling Engine; Methodology for a Comparative Study of its Performance and Sensitivity Analysis in Recreational Sailing Boats in Different European Climates
Authors: Rey Guillermo , Ulloa Carlos , Míguez Jose , Arce Elena
Published: March 2016

Micro combined heating and power (micro-CHP) systems are becoming more than important, and even essential, if we pretend to take full advantage of available energy. The efficiency of this kind of systems reaches 90% and important savings in energy transport processes can occur. In this research, an internal combustion engine (ICE)-based micro-CHP system was developed and tested under specific constraints. The system uses a two cylinder Otto engine as prime mover, coupled to an electrical alternator, and it uses exhaust gases and engine cooling circuit heat. The micro-CHP system was developed to match the electrical power of a typical Stirling engine (SE)-based micro-CHP unit, in order to later compare both systems’ performance under similar circumstances. Different operating modes were tested under different engine speeds, in order to find the optimum operating point. A stand-alone portable application of this system was performed using recreational sailing boats as mobile homes. Specific considerations had to be taken, related to boundary conditions with sea water, and a transient simulation was performed, considering the boat under three different European climates. Results were compared for the different locations and the performance of the equipment shown. A comparative study with the SE-based micro-CHP system performance was done, and a sensitivity analysis of the influence of the battery size was carried out under the same conditions. The SE and ICE-based proposed micro-CHP system have similar behavior, except for the differences found due to the electric/thermal power ratios in both systems. Battery bank size sensitivity analysis reflects a limit in performance improvement. This limit is caused by the uniform distribution of electrical demand profile.
Towards Renewable Iodide Sources for Electrolytes in Dye-Sensitized Solar Cells
Authors: Sagaidak Iryna , Huertas Guillaume , Nguyen Van Nhien Albert , Sauvage Frédéric
Published: March 2016

A novel family of iodide salts and ionic liquids based on different carbohydrate core units is herein described for application in dye-sensitized solar cell (DSC). The influence of the molecular skeleton and the cationic structure on the electrolyte properties, device performance and on interfacial charge transfer has been investigated. In combination with the C106 polypyridyl ruthenium sensitizer, power conversion efficiencies lying between 5.0% and 7.3% under standard Air Mass (A.M.) 1.5G conditions were obtained in association with a low volatile methoxypropionitrile (MPN)-based electrolyte.
A Novel Secondary Control for Microgrid Based on Synergetic Control of Multi-Agent System
Authors: Yu Zhiwen , Ai Qian , Gong Jinxia , Piao Longjian
Published: March 2016

In power systems, the secondary control is a very useful way to restore the system frequency and voltage to the rated value. This paper tries to propose a secondary frequency and voltage control of islanded microgrids based on the distributed synergetic control of multi-agent systems. In the proposed control, since each distributed generation only requires its own information and that of the neighbors, the secondary control is fully distributed. The system is more reliable because the central controller and complex communication network are reduced in the distributed structure. Based on multi-agent systems, the dynamic model is established, and distributed synergetic control algorithms are given to design the secondary control of the islanded microgrid. Meanwhile, the system has globally asymptotic stability under the proposed control, which is proved by the direct Lyapunov method. Simulation results about a test microgrid are given to verify the effectiveness of the proposed control.
Consumer Choice between Food Safety and Food Quality: The Case of Farm-Raised Atlantic Salmon
Authors: Haghiri Morteza
Published: March 2016

Since the food incidence of polychlorinated biphenyls in farm-raised Atlantic salmon, its market demand has drastically changed as a result of consumers mistrust in both the quality and safety of the product. Policymakers have been trying to find ways to ensure consumers that farm-raised Atlantic salmon is safe. One of the suggested policies is the implementation of integrated traceability methods and quality control systems. This article examines consumer choice between food safety and food quality to purchase certified farm-raised Atlantic salmon, defined as a product that has passed through various stages of traceability systems in the province of Newfoundland and Labrador, Canada.
“Spectacular Death”—Proposing a New Fifth Phase to Philippe Ariès’s Admirable History of Death
Authors: Jacobsen Michael
Published: March 2016

This article revisits, reviews and revises the much cited and magisterial description of successive historical death mentalities from the Middle Ages to modern society as proposed several decades ago by French historian Philippe Ariès. The article first outlines Ariès’s position starting out with the medieval “tamed death,” then moves on to point to several inherent limitations in his history-writing, before suggesting a revision and update of it. Whereas Ariès ended his history-writing with modern “forbidden death,” the author suggests that contemporary death mentality in Western society rather be labelled “spectacular death” in which death, dying and mourning have increasingly become spectacles. Moreover, the author proposes that what is currently happening in contemporary Western society can be interpreted as an expression of a “partial re-reversal” of “forbidden death” to some of the characteristic features of previous historical death mentalities, which—coupled with contemporary scientific and technological possibilities—creates several paradoxical tendencies making death linger uneasily between liberation and denial as well as between autonomy and control.
A Review of the Water and Energy Sectors and the Use of a Nexus Approach in Abu Dhabi
Authors: Paul Parneet , Al Tenaiji Ameena , Braimah Nuhu
Published: March 2016

Rapid population increase coupled with urbanization and industrialization has resulted in shortages of water in the Middle East. This situation is further exacerbated by global climate change due to greenhouse gas emissions. Recent research advocates that solutions to the global water security and scarcity crisis must involve water–energy nexus approaches. This means adopting policies and strategies that harmonize these inter-related sectors to minimize environmental impact while maximizing human benefit. In the case of Abu Dhabi, when designing and locating oil/gas refineries and associated power generation facilities, previous relevant decisions were based on simple economic and geographical grounds, such as nearness to oil rigs, pipelines, existing industries and port facilities, etc. The subsequent design and location of water abstraction and treatment works operated by the waste heat from these refining and/or power generation processes was catered for as an afterthought, meaning that there is now a mismatch between the water and energy supplies and demands. This review study was carried out to show how Abu Dhabi is trying now to integrate its water–energy sectors using a nexus approach so that future water/power infrastructure is designed optimally and operated in harmony, especially in regard to future demand. Based upon this review work, some recommendations are made for designers and policy makers alike to bolster the nexus approach that Abu Dhabi is pursuing.
Association between Atmospheric Fine Particulate Matter and Hospital Admissions for Chronic Obstructive Pulmonary Disease in Southwestern Taiwan: A Population-Based Study
Authors: Hwang Su-Lun , Guo Su-Er , Chi Miao-Ching , Chou Chiang-Ting , Lin Yu-Ching , Lin Chieh-Mo , Chou Yen-Li
Published: March 2016

Objectives: This paper reports on the findings of a population-based study to evaluate the relationship between atmospheric fine particulate matter (PM2.5) levels and hospital admissions for chronic obstructive pulmonary disease (COPD) in southwestern Taiwan over a three-year period, 2008–2010. Methods: Data on hospital admissions for COPD and PM2.5 levels were obtained from the National Health Insurance Research database (NHIRD) and the Environmental Protection Administration from 2008 to 2010, respectively. The lag structure of relative risks (RRs) of hospital admissions for COPD was estimated using a Poisson regression model. Results: During the study period, the overall average hospitalization rate of COPD and mean 24-h average level of PM2.5 was 0.18% and 39.37 μg/m3, respectively. There were seasonal variations in PM2.5 concentrations in southwestern Taiwan, with higher PM2.5 concentrations in both spring (average: 48.54 μg/m3) and winter (49.96 μg/m3) than in summer (25.89 μg/m3) and autumn (33.37 μg/m3). Increased COPD admissions were significantly associated with PM2.5 in both spring (February–April) and winter (October–January), with the relative risks (RRs) for every 10 μg/m3 increase in PM2.5 being 1.25 (95% CI = 1.22–1.27) and 1.24 (95% CI = 1.23–1.26), respectively, at a lag zero days (i.e., no lag days). Lag effects on COPD admissions were observed for PM2.5, with the elevated RRs beginning at lag zero days and larger RRs estimates tending to occur at longer lags (up to six days, i.e., lag 0–5 days). Conclusions: In general, findings reveal an association between atmospheric fine particulate matter (PM2.5) and hospital admissions for COPD in southwestern Taiwan, especially during both spring and winter seasons.
A Neurophysiological Approach for Evaluating Noise-Induced Sleep Disturbance: Calculating the Time Constant of the Dynamic Characteristics in the Brainstem
Authors: Tagusari Junta , Matsui Toshihito
Published: March 2016

Chronic sleep disturbance induced by traffic noise is considered to cause environmental sleep disorder, which increases the risk of cardiovascular disease, stroke, diabetes and other stress-related diseases. However, noise indices for the evaluation of sleep disturbance are not based on the neurophysiological process of awakening regulated by the brainstem. In this study, through the neurophysiological approach, we attempted (1) to investigate the thresholds of awakening due to external stimuli in the brainstem; (2) to evaluate the dynamic characteristics in the brainstem and (3) to verify the validity of existing noise indices. Using the mathematical Phillips–Robinson model, we obtained thresholds of awakening in the brainstem for different durations of external stimuli. The analysis revealed that the brainstem seemed insensitive to short stimuli and that the response to external stimuli in the brainstem could be approximated by a first-order lag system with a time constant of 10–100 s. These results suggest that the brainstem did not integrate sound energy as external stimuli, but neuroelectrical signals from auditory nerve. To understand the awakening risk accumulated in the brainstem, we introduced a new concept of “awakening potential” instead of sound energy.
Identification of Haplotype Tag Single-Nucleotide Polymorphisms within the PPAR Family Genes and Their Clinical Relevance in Patients with Major Trauma
Authors: Gao Jun-Wei , Zeng Ling , Zhang An-Qiang , Wang Xiao , Pan Wei , Du Ding-Yuan , Zhang Lian-Yang , Gu Wei , Jiang Jian-Xin
Published: March 2016

Background: Peroxisome proliferator-activated receptors (PPARs) play important roles in the development of inflammatory diseases and sepsis. Recently, genetic variants of PPARs genes have been widely studied in some inflammatory diseases. However, the association between PPAR family of genes polymorphisms and sepsis risk in trauma patients was little known. Methods: SNPs were selected from the PPARs genes through constructing haplotype blocks and genotyped by the improved multiplex ligation detection reaction (iMLDR) method. The association between the selected SNPs and the risk of sepsis and multiple organ dysfunction (MOD) scores was evaluated in 734 trauma patients. In addition, tumor necrosis factor α (TNFα) production of peripheral blood leukocytes was also analyzed after lipopolysaccharide (LPS) stimulation. Results: Our results revealed that there were significant associations between the rs10865710 polymorphism and the risk of sepsis and MOD scores in Chinese Han trauma patients. Further, we found that the level of TNFα production was higher in patients with the rs10865710 G allele compared to those with the variant C allele. Conclusions: The rs10865710 polymorphism in the PPARγ gene might be used to assess the risk of sepsis and multiple organ dysfunction syndrome (MODS) in trauma patients.
Enhanced Co-Worker Social Support in Isolated Work Groups and Its Mitigating Role on the Work-Family Conflict-Depression Loss Spiral
Authors: McTernan Wesley , Dollard Maureen , Tuckey Michelle , Vandenberg Robert
Published: March 2016

This paper examines a loss spiral model (i.e., reciprocal relationships) between work-family conflict and depression, moderated by co-worker support. We expected that the moderation effect due to co-worker support would be evident among those working in isolation (i.e., mining workers) due to a greater level of intragroup attraction and saliency attributable to the proximity effects. We used a two wave panel study and data from a random population sample of Australian employees (n = 2793, [n = 112 mining, n = 2681 non-mining]). Using structural equation modelling we tested the reciprocal three way interaction effects. In line with our theory, co-worker support buffered the reciprocal relationship between WFC and depression, showing a protective effect in both pathways. These moderation effects were found in the mining industry only suggesting a proximity component moderates the social support buffer hypothesis (i.e., a three way interaction effect). The present paper integrates previous theoretical perspectives of stress and support, and provides insight into the changing dynamics of workplace relationships.
Children’s Exposure to Radon in Nursery and Primary Schools
Authors: Branco Pedro , Nunes Rafael , Alvim-Ferraz Maria , Martins Fernando , Sousa Sofia
Published: March 2016

The literature proves an evident association between indoor radon exposure and lung cancer, even at low doses. This study brings a new approach to the study of children’s exposure to radon by aiming to evaluate exposure to indoor radon concentrations in nursery and primary schools from two districts in Portugal (Porto and Bragança), considering different influencing factors (occupation patterns, classroom floor level, year of the buildings’ construction and soil composition of the building site), as well as the comparison with IAQ standard values for health protection. Fifteen nursery and primary schools in the Porto and Bragança districts were considered: five nursery schools for infants and twelve for pre-schoolers (seven different buildings), as well as eight primary schools. Radon measurements were performed continuously. The measured concentrations depended on the building occupation, classroom floor level and year of the buildings’ construction. Although they were in general within the Portuguese legislation for IAQ, exceedances to international standards were found. These results point out the need of assessing indoor radon concentrations not only in primary schools, but also in nursery schools, never performed in Portugal before this study. It is important to extend the study to other microenvironments like homes, and in time to estimate the annual effective dose and to assess lifetime health risks.
Nutritional Signaling via Free Fatty Acid Receptors
Authors: Miyamoto Junki , Hasegawa Sae , Kasubuchi Mayu , Ichimura Atsuhiko , Nakajima Akira , Kimura Ikuo
Published: March 2016

Excess energy is stored primarily as triglycerides, which are mobilized when demand for energy arises. Dysfunction of energy balance by excess food intake leads to metabolic diseases, such as obesity and diabetes. Free fatty acids (FFAs) provided by dietary fat are not only important nutrients, but also contribute key physiological functions via FFA receptor (FFAR)-mediated signaling molecules, which depend on FFAs’ carbon chain length and the ligand specificity of the receptors. Functional analyses have revealed that FFARs are critical for metabolic functions, such as peptide hormone secretion and inflammation, and contribute to energy homeostasis. In particular, recent studies have shown that the administration of selective agonists of G protein-coupled receptor (GPR) 40 and GPR120 improved glucose metabolism and systemic metabolic disorders. Furthermore, the anti-inflammation and energy metabolism effects of short chain FAs have been linked to the activation of GPR41 and GPR43. In this review, we summarize recent progress in research on FFAs and their physiological roles in the regulation of energy metabolism.
Mechanism of Sleep Disturbance in Children with Atopic Dermatitis and the Role of the Circadian Rhythm and Melatonin
Authors: Chang Yung-Sen , Chiang Bor-Luen
Published: March 2016

Sleep disturbance is common in children with atopic dermatitis (AD). It is a major factor leading to impaired quality of life in these patients and could have negative effects on neurocognitive function and behavior. However, the pathophysiology of sleep disturbance in children with AD is poorly understood, and there is no consensus on how to manage sleep problems in these patients. Pruritus and scratching could lead to sleep disruption but is unlikely the sole etiology. The circadian rhythm of cytokines, the immune system, and skin physiology such as transcutaneous water loss and skin blood flow might also play a role. Recent studies have suggested that melatonin could also be involved due to its multiple effects on sleep, immunomodulation, and anti-oxidant ability. Environmental factors should also be considered. In this review, we summarize the current understanding of the pathophysiology of sleep disturbance in children with AD, and discuss possible therapeutic implications.
Thymoquinone Modulates Blood Coagulation in Vitro via Its Effects on Inflammatory and Coagulation Pathways
Authors: Muralidharan-Chari Vandhana , Kim Jaehan , Abuawad Ahlam , Naeem Mubeena , Cui Huadong , Mousa Shaker
Published: March 2016

Thymoquinone (THQ) is a major component of black seeds. Given that both THQ and black seeds exhibit anti-cancer and anti-inflammatory activities, we hypothesized that THQ will affect cancer-associated thrombosis (CAT), which is primarily triggered by tissue factor (TF) and inflammation. The effect of both black seed-extracted and purchased (“pure”) THQ on normal blood coagulation was tested with in vitro thromboelastography (TEG) and activated partial thromboplastin time (aPTT) coagulation assays. The effect of pure THQ on CAT was tested with aPTT assay using pancreatic cancer cell lines that are either positive or negative for TF, and with TEG assay using lipopolysaccharide as an inflammatory trigger. Additionally, the direct effect of THQ on the inactivation of factors IIa and Xa was assessed. Since TNF-α facilitates crosstalk between inflammation and thrombosis by triggering the NF-κB pathway, we tested THQ’s ability to interfere with this communication with a luciferase assay. Both extracted and pure THQ had minimal effects on normal blood coagulation. Pure THQ reversed CAT initiated by both TF and inflammation to basal levels (p < 0.001). Mechanistically, while THQ had minimal to no effect on factor IIa and Xa inactivation, it strongly reduced the effects of TNF-α on NF-κB elements (p < 0.001). THQ has a minimal effect on basal coagulation and can reverse CAT in vitro, possibly by interfering with the crosstalk between inflammation and coagulation. This study suggests the utility of THQ as a preventative anticoagulant and/or as a supplement to existing chemotherapies and anticoagulant therapies.
On-Body Smartphone Localization with an Accelerometer
Authors: Fujinami Kaori
Published: March 2016

A user of a smartphone may feel convenient, happy, safe, etc., if his/her smartphone works smartly based on his/her context or the context of the device. In this article, we deal with the position of a smartphone on the body and carrying items like bags as the context of a device. The storing position of a smartphone impacts the performance of the notification to a user, as well as the measurement of embedded sensors, which plays an important role in a device’s functionality control, accurate activity recognition and reliable environmental sensing. In this article, nine storing positions, including four types of bags, are subject to recognition using an accelerometer on a smartphone. In total, 63 features are selected as a set of features among 182 systematically-defined features, which can characterize and discriminate the motion of a smartphone terminal during walking. As a result of leave-one-subject-out cross-validation, an accuracy of 0.801 for the nine-class classification is shown, while an accuracy of 0.859 is obtained against five classes, which merges the subclasses of trouser pockets and bags. We also show the basic performance evaluation to select the proper window size and classifier. Furthermore, the analysis of the contributive features is presented.
Enabling Virtual Sensing as a Service
Authors: Li Yang , Pandis Ioannis , Guo Yike
Published: March 2016

In many situations, placing a physical sensor in the ideal position in or on the human body to acquire sensing data is incredibly difficult. Virtual sensors, in contrast to physical sensors, can provide indirect measurements by making use of other available sensor data. In this paper, we demonstrate a virtual sensing application developed as a service on top of a cloud-based health sensor data management platform called Wiki-Health. The proposed application “implants” virtual sensors in the human body by integrating environmental, geographic and personal sensor data with physiological models to compute temperature estimations of various parts of the body. The feasibility of the proposed virtual sensing service is supported by a case study. The ability to share computational models relevant to do calculations on measured data on the go is also discussed.
Functional and Comparative Genomics of Hoxa2 Gene cis-Regulatory Elements: Evidence for Evolutionary Modification of Ancestral Core Element Activity
Authors: Davis Adam , Reubens Michael , Stellwag Edmund
Published: March 2016

Hoxa2 is an evolutionarily conserved developmental regulatory gene that functions to specify rhombomere (r) and pharyngeal arch (PA) identities throughout the Osteichthyes. Japanese medaka (Oryzias latipes) hoxa2a, like orthologous Hoxa2 genes from other osteichthyans, is expressed during embryogenesis in r2–7 and PA2-7, whereas the paralogous medaka pseudogene, ψhoxa2b, is expressed in noncanonical Hoxa2 domains, including the pectoral fin buds. To understand the evolution of cis-regulatory element (CRE) control of gene expression, we conducted eGFP reporter gene expression studies with extensive functional mapping of several conserved CREs upstream of medaka hoxa2a and ψhoxa2b in transient and stable-line transgenic medaka embryos. The CREs tested were previously shown to contribute to directing mouse Hoxa2 gene expression in r3, r5, and PA2-4. Our results reveal the presence of sequence elements embedded in the medaka hoxa2a and ψhoxa2b upstream enhancer regions (UERs) that mediate expression in r4 and the PAs (hoxa2a r4/CNCC element) or in r3–7 and the PAs ψhoxa2b r3–7/CNCC element), respectively. Further, these elements were shown to be highly conserved among osteichthyans, which suggests that the r4 specifying element embedded in the UER of Hoxa2 is a deeply rooted rhombomere specifying element and the activity of this element has been modified by the evolution of flanking sequences that redirect its activity to alternative developmental compartments.
Personal Genome Sequencing in Ostensibly Healthy Individuals and the PeopleSeq Consortium
Authors: Linderman Michael , Nielsen Daiva , Green Robert
Published: March 2016

Thousands of ostensibly healthy individuals have had their exome or genome sequenced, but a much smaller number of these individuals have received any personal genomic results from that sequencing. We term those projects in which ostensibly healthy participants can receive sequencing-derived genetic findings and may also have access to their genomic data as participatory predispositional personal genome sequencing (PPGS). Here we are focused on genome sequencing applied in a pre-symptomatic context and so define PPGS to exclude diagnostic genome sequencing intended to identify the molecular cause of suspected or diagnosed genetic disease. In this report we describe the design of completed and underway PPGS projects, briefly summarize the results reported to date and introduce the PeopleSeq Consortium, a newly formed collaboration of PPGS projects designed to collect much-needed longitudinal outcome data.
Synthesis of Dendronized Poly(l-Glutamate) via Azide-Alkyne Click Chemistry
Authors: Perdih Peter , Kržan Andrej , Žagar Ema
Published: March 2016

Poly(l-glutamate) (PGlu) was modified with a second-generation dendron to obtain the dendronized polyglutamate, P(Glu-D). Synthesized P(Glu-D) exhibited a degree of polymerization (DPn) of 46 and a 43% degree of dendronization. Perfect agreement was found between the P(Glu-D) expected structure and the results of nuclear magnetic resonance spectroscopy (NMR) and size-exclusion chromatography coupled to a multi-angle light-scattering detector (SEC-MALS) analysis. The PGlu precursor was modified by coupling with a bifunctional building block (N3-Pr-NH2) in the presence of 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) coupling reagent. The second-generation polyamide dendron was prepared by a stepwise procedure involving the coupling of propargylamine to the l-lysine carboxyl group, followed by attaching the protected 2,2-bis(methylol)propionic acid (bis-MPA) building block to the l-lysine amino groups. The hydroxyl groups of the resulting second-generation dendron were quantitatively deprotected under mild acidic conditions. The deprotected dendron with an acetylene focal group was coupled to the pendant azide groups of the modified linear copolypeptide, P(Glu-N3), in a Cu(I) catalyzed azide-alkyne cycloaddition reaction to form a 1,4-disubstituted triazole. The dendronization reaction proceeded quantitatively in 48 hours in aqueous medium as confirmed by 1H NMR and Fourier transform infrared spectroscopy (FT-IR) spectroscopy.
Enhanced Photocatalytic Activity of CdS-Decorated TiO2/Carbon Core-Shell Microspheres Derived from Microcrystalline Cellulose
Authors: Liu Xin , Li Yinliang , Yang Jun , Wang Bo , Ma Mingguo , Xu Feng , Sun Runcang , Zhang Xueming
Published: March 2016

The fabrication of reusable and biodegradation materials from renewable resources such as cellulose is essential for a sustainable world. The core-shell structured CdS-decorated TiO2/Carbon microspheres (CdS/TiO2/Carbon MS) photocatalyst was synthesized with controlled hydrolysis and a novel sonochemical method. It was prepared by using crosslinked microcrystalline cellulose as the core, tetrabutyl titanate as the titania source and CdS as the photosensitizer. The morphology, chemical structure and properties of the obtained material were characterized by many means. Additionally, the photocatalytic activity of the CdS/TiO2/Carbon MS was evaluated by the photodegradation efficiency of Rhodamine B solution, which reached 95.24% under visible light irradiation. This study demonstrated the excellent photocatalytic performance of CdS/TiO2/Carbon MS, which might have promising applications in environmental treatments.
Contemporary Strategies for the Synthesis of Tetrahydropyran Derivatives: Application to Total Synthesis of Neopeltolide, a Marine Macrolide Natural Product
Authors: Fuwa Haruhiko
Published: March 2016

Tetrahydropyrans are structural motifs that are abundantly present in a range of biologically important marine natural products. As such, significant efforts have been paid to the development of efficient and versatile methods for the synthesis of tetrahydropyran derivatives. Neopeltolide, a potent antiproliferative marine natural product, has been an attractive target compound for synthetic chemists because of its complex structure comprised of a 14-membered macrolactone embedded with a tetrahydropyran ring, and twenty total and formal syntheses of this natural product have been reported so far. This review summarizes the total and formal syntheses of neopeltolide and its analogues, highlighting the synthetic strategies exploited for constructing the tetrahydropyran ring.
Fucoxanthin, a Marine Carotenoid, Reverses Scopolamine-Induced Cognitive Impairments in Mice and Inhibits Acetylcholinesterase in Vitro
Authors: Lin Jiajia , Huang Ling , Yu Jie , Xiang Siying , Wang Jialing , Zhang Jinrong , Yan Xiaojun , Cui Wei , He Shan , Wang Qinwen
Published: March 2016

Fucoxanthin, a natural carotenoid abundant in edible brown seaweeds, has been shown to possess anti-cancer, anti-oxidant, anti-obesity and anti-diabetic effects. In this study, we report for the first time that fucoxanthin effectively protects against scopolamine-induced cognitive impairments in mice. In addition, fucoxanthin significantly reversed the scopolamine-induced increase of acetylcholinesterase (AChE) activity and decreased both choline acetyltransferase activity and brain-derived neurotrophic factor (BDNF) expression. Using an in vitro AChE activity assay, we discovered that fucoxanthin directly inhibits AChE with an IC50 value of 81.2 μM. Molecular docking analysis suggests that fucoxanthin likely interacts with the peripheral anionic site within AChE, which is in accordance with enzymatic activity results showing that fucoxanthin inhibits AChE in a non-competitive manner. Based on our current findings, we anticipate that fucoxanthin might exhibit great therapeutic efficacy for the treatment of Alzheimer’s disease by acting on multiple targets, including inhibiting AChE and increasing BDNF expression.
In Vitro Anticancer Activity of Phlorofucofuroeckol A via Upregulation of Activating Transcription Factor 3 against Human Colorectal Cancer Cells
Authors: Eo Hyun , Kwon Tae-Hyung , Park Gwang , Song Hun , Lee Su-Jin , Park Nyun-Ho , Jeong Jin
Published: March 2016

Phlorofucofuroeckol A (PFF-A), one of the phlorotannins found in brown algae, has been reported to exert anti-cancer property. However, the molecular mechanism for the anti-cancer effect of PFF-A has not been known. Activating transcription factor 3 (ATF3) has been reported to be associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which PFF-A stimulates ATF3 expression and apoptosis in human colorectal cancer cells. PFF-A decreased cell viability through apoptosis of human colorectal cancer cells. PFF-A increased ATF3 expression through regulating transcriptional activity. The responsible cis-element for ATF3 transcriptional activation by PFF-A was cAMP response element binding protein (CREB), located between positions −147 and −85 of the ATF3 promoter. Inhibition of p38, c-Jun N-terminal kinases (JNK), glycogen synthase kinase (GSK) 3β, and IκB kinase (IKK)-α blocked PFF-A-mediated ATF3 expression. ATF3 knockdown by ATF3 siRNA attenuated the cleavage of poly (ADP-ribose) polymerase (PARP) by PFF-A, while ATF3 overexpression increased PFF-A-mediated cleaved PARP. These results suggest that PFF-A may exert anti-cancer property through inducing apoptosis via the ATF3-mediated pathway in human colorectal cancer cells.
Surface Tension Directed Fluidic Self-Assembly of Semiconductor Chips across Length Scales and Material Boundaries
Authors: Biswas Shantonu , Mozafari Mahsa , Stauden Thomas , Jacobs Heiko
Published: March 2016

This publication provides an overview and discusses some challenges of surface tension directed fluidic self-assembly of semiconductor chips which are transported in a liquid medium. The discussion is limited to surface tension directed self-assembly where the capture, alignment, and electrical connection process is driven by the surface free energy of molten solder bumps where the authors have made a contribution. The general context is to develop a massively parallel and scalable assembly process to overcome some of the limitations of current robotic pick and place and serial wire bonding concepts. The following parts will be discussed: (2) Single-step assembly of LED arrays containing a repetition of a single component type; (3) Multi-step assembly of more than one component type adding a sequence and geometrical shape confinement to the basic concept to build more complex structures; demonstrators contain (3.1) self-packaging surface mount devices, and (3.2) multi-chip assemblies with unique angular orientation. Subsequently, measures are discussed (4) to enable the assembly of microscopic chips (10 μm–1 mm); a different transport method is introduced; demonstrators include the assembly of photovoltaic modules containing microscopic silicon tiles. Finally, (5) the extension to enable large area assembly is presented; a first reel-to-reel assembly machine is realized; the machine is applied to the field of solid state lighting and the emerging field of stretchable electronics which requires the assembly and electrical connection of semiconductor devices over exceedingly large area substrates.
NMR Study on the Inclusion Complexes of β-Cyclodextrin with Isoflavones
Authors: Zhao Rui , Sandström Corine , Zhang Haiyang , Tan Tianwei
Published: March 2016

The structure of the inclusion complexes of β-cyclodextrin (β-CD) with daidzein and daidzin in D2O were investigated using NMR spectroscopy. For the β-CD and daidzein system, two types of 1:1 complexes were formed with the daidzein deeply inserted into the CD cavity with different orientations. For the β-CD/daidzin system, a 1:1 complex was formed with the flavonoid part of daidzin entering the CD cavity from the wide rim. The inclusion complexes determined by NMR were constructed using molecular docking. Furthermore, the mixture of puerarin, daidzein and daidzin, which are the major isoflavonoid components present in Radix puerariae, was analyzed by diffusion-ordered spectroscopy (DOSY) alone and upon addition of β-CD in order to mimic chromatographic conditions and compare their binding affinities.
The Chemical Composition of Achillea wilhelmsii C. Koch and Its Desirable Effects on Hyperglycemia, Inflammatory Mediators and Hypercholesterolemia as Risk Factors for Cardiometabolic Disease
Authors: Khazneh Elian , Hřibová Petra , Hošek Jan , Suchý Pavel , Kollár Peter , Pražanová Gabriela , Muselík Jan , Hanaková Zuzana , Václavík Jiří , Miłek Michał , Legáth Jaroslav , Šmejkal Karel
Published: March 2016

This study was done to identify the content compounds of Achillea wilhelmsii (A. wilhelmsii) and to evaluate its hypoglycemic and anti-hypercholesterolemic activity and effect on inflammatory mediators. The extracts and fractions of A. wilhelmsii were thoroughly analyzed using high performance liquid chromatography (HPLC), and the total content of phenols and flavonoids was determined. The hypoglycemic activity was evaluated in vivo using alloxan-induced diabetic mice. The effect upon inflammatory mediators was evaluated in vitro using the human monocytic leukemia cell line (THP-1). The anti-hypercholesterolemic activity was evaluated in vitro using the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase assay kit. The water extract (WE)-treated group showed the highest reduction in the fasting blood glucose levels (FBGL). The chloroform fraction (CF) and ethyl acetate fraction (EAF) both showed a significant ability to reduce the secretion of tumor necrosis factor alpha (TNF-α). The EAF, however, also attenuated the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). The CF showed the most significant 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) inhibition activity. The five main compounds in the CF were isolated and identified. Out of the five compounds in the CF, 1β,10β-epoxydesacetoxymatricarin (CP1) and leucodin (CP2) showed the highest anti-hypercholesterolemic potential. A molecular docking study provided corresponding results.
Mechanistic Insight of Bivalent Compound 21MO as Potential Neuroprotectant for Alzheimer’s Disease
Authors: Saathoff John , Liu Kai , Chojnacki Jeremy , He Liu , Chen Qun , Lesnefsky Edward , Zhang Shijun
Published: March 2016

We have recently developed a bivalent strategy to provide novel compounds that potentially target multiple risk factors involved in the development of Alzheimer’s disease (AD). Our previous studies employing a bivalent compound with a shorter spacer (17MN) implicated that this compound can localize into mitochondria and endoplasmic reticulum (ER), thus interfering with the change of mitochondria membrane potential (MMP) and Ca2+ levels in MC65 cells upon removal of tetracycline (TC). In this report, we examined the effects by a bivalent compound with a longer spacer (21MO) in MC65 cells. Our results demonstrated that 21MO suppressed the change of MMP, possibly via interaction with the mitochondrial complex I in MC65 cells. Interestingly, 21MO did not show any effects on the Ca2+ level upon TC removal in MC65 cells. Our previous studies suggested that the mobilization of Ca2+ in MC65 cells, upon withdraw of TC, originated from ER, so the results implicated that 21MO may preferentially interact with mitochondria in MC65 cells under the current experimental conditions. Collectively, the results suggest that bivalent compounds with varied spacer length and cell membrane anchor moiety may exhibit neuroprotective activities via different mechanisms of action.