bluuebunny/crossref_metadata_2025 huggingface dataset:
Homoeologous Chromosome Pairing and Alien Introgression in Backcrossing Progenies Derived from Hybrids Solanum tuberosum (+) Mexican 2x (1 EBN) B-Genome Potato Species
Authors: Gavrilenko Tatjana , Pendinen Galina , Antonova Olga , Makarova Tamara , Thieme Ramona
Published: July 2023

We applied a genomic in situ hybridization (GISH) to analyze the genomic constitution of and meiotic pairing in interspecific somatic hybrids, and in a wide subset of backcrossing derivatives (BC1–BC5), from three interspecific combinations involving the cultivated potato, Solanum tuberosum (AAAA genome), and three diploid (1 EBN) wild Mexican potato species (genome BB)—S. tarnii, S. pinnatisectum, and S. bulbocastanum. The theoretically expected genomic composition was detected in the somatic hybrids (AAAABB) and in the BC1 progeny (AAAAB), whereas in the subsequent BC2–BC4 generations, the partial loss of alien chromosomes was observed and almost all the BC5 genotypes showed a complete chromosome elimination of wild species. GISH revealed a homoeologous pairing between the chromosomes of the A- and the B-genomes in each of the hybrid progenies. Using GISH, we selected introgression lines with a single chromosome of the wild species in a potato genome background, as well as introgression lines with intergenomic recombinant chromosomes. Moreover, via molecular screening, BC hybrids with diagnostic markers for the R-genes conferring resistance to late blight disease and to the quarantine pest of the potato–Columbia root-knot nematode—were selected. The potential application of the results obtained for the planning of introgressive schemes directed to the breeding of advanced lines with multiple disease and pest resistance is discussed.
Performance Analysis and Testing of a Multi-Duct Orchard Sprayer
Authors: Li Zhanbiao , Wang Xingyu , Li Cui , Lan Haipeng , He Yichuan , Tang Zhihui , Tang Yurong
Published: July 2023

A multi-duct orchard sprayer is designed in this paper to address the problem of low droplet coverage during plant protection in walnut orchards in South Xinjiang, China. This spray comprises a base frame, a pesticide tank, an air delivery system, a copying base frame and a drive system. Effects of the sprayer structure on the flow field were simulated using computational fluid dynamics. Operation parameters were optimised using the response surface analysis and validated using a field test. The results demonstrated that five jet tubes in the simulation design could fulfil the design requirements of GB/T 32250.3-2022. The upper, middle and lower droplet coverages were 74.92%, 90.01% and 69.9%, respectively, when the sprayer’s advancing speed, the angle of jet tubes and the nozzle diameter were 0.53 m/s, 70.27° and 0.81 mm, respectively. The machine can effectively enhance the spraying efficiency and droplet coverage of each canopy during plant protection operations in the new walnut orchards of South Xinjiang, China, and provide references for designing and optimising fruit tree spraying machinery.
Research on Apple Object Detection and Localization Method Based on Improved YOLOX and RGB-D Images
Authors: Hu Tiantian , Wang Wenbo , Gu Jinan , Xia Zilin , Zhang Jian , Wang Bo
Published: July 2023

The vision-based fruit recognition and localization system is the basis for the automatic operation of agricultural harvesting robots. Existing detection models are often constrained by high complexity and slow inference speed, which do not meet the real-time requirements of harvesting robots. Here, a method for apple object detection and localization is proposed to address the above problems. First, an improved YOLOX network is designed to detect the target region, with a multi-branch topology in the training phase and a single-branch structure in the inference phase. The spatial pyramid pooling layer (SPP) with serial structure is used to expand the receptive field of the backbone network and ensure a fixed output. Second, the RGB-D camera is used to obtain the aligned depth image and to calculate the depth value of the desired point. Finally, the three-dimensional coordinates of apple-picking points are obtained by combining two-dimensional coordinates in the RGB image and depth value. Experimental results show that the proposed method has high accuracy and real-time performance: F1 is 93%, mean average precision (mAP) is 94.09%, detection speed can reach 167.43 F/s, and the positioning errors in X, Y, and Z directions are less than 7 mm, 7 mm, and 5 mm, respectively.
Does Precision Technologies Adoption Contribute to the Economic and Agri-Environmental Sustainability of Mediterranean Wheat Production? An Italian Case Study
Authors: Finco Adele , Bentivoglio Deborah , Belletti Matteo , Chiaraluce Giulia , Fiorentini Marco , Ledda Luigi , Orsini Roberto
Published: July 2023

The European Green Deal has set a concrete strategic plan to increase farm sustainability. At the same time, the current global challenges, due to climate change and fuels and commodity market crises, combined with the COVID-19 pandemic and the ongoing war in Ukraine, affect the need for quality food and necessitate the reduction of negative external effects of agricultural production, with fair remuneration for the farmers. In response, precision agriculture has great potential to contribute to sustainable development. Precision agriculture is a farming management system that provides a holistic approach to managing the spatial and temporal crop and soil variability within a field to improve the farm’s performance and sustainability. However, farmers are still hesitant to adopt it. On these premises, the study aims to evaluate the impacts of precision agriculture technologies on farm economic, agronomic, and environmental management by farmers adopting (or not) these technologies, using the case study method. In detail, the work focuses on the period 2014–2022 for two farms that cultivate durum wheat in central Italy. The results suggest that the implementation of precision technologies can guarantee economic and agri-environmental efficiency. The results could serve as a basis for developing a program to start training in farms as well as to suggest policy strategies.
Maize Grain Germination Is Accompanied by Acidification of the Environment
Authors: Wellmann Konrad , Varnskühler Jens , Leubner-Metzger Gerhard , Mummenhoff Klaus
Published: July 2023

Seed germination is a complex process involving several stages, starting with the imbibition of water and ending with the emergence of the radicle. In the current study, we address the observation of an unexpected pH shift during the imbibition of maize grains. We used direct pH measurements of soak water, the pH indicator methyl red, and anatomical analysis to shed light on the acidification associated with maize (Zea mays L.) germination, a largely overlooked phenomenon. Our work shows that acidification during imbibition of maize grains is a two-step process: (i) early, rapid acidification (pH values up to 4.4), in which protons stored in the (dead) pericarp/testa are mobilised and rapidly diffuse into the surrounding medium, and (ii) late, delayed acidification (pH values just below 6), starting hours after contact of grains with water, representing an active transport process caused by living cells of the seed. We discuss the physiological mechanisms and ecological relevance of environmental acidification during maize grain germination.
Open-Field Agrivoltaic System Impacts on Photothermal Environment and Light Environment Simulation Analysis in Eastern China
Authors: Zhang Long , Yang Zhipeng , Wu Xue , Wang Wenju , Yang Chen , Xu Guijun , Wu Cuinan , Bao Encai
Published: July 2023

In order to clarify the temporal and spatial changes in the internal photothermal environment in an open-field agrivoltaic system (OAVS), this paper took the OAVS in eastern China as the research object and divided the internal area into the southern area, middle area and northern area, according to the spatial structure. Further, a photothermal environment test was conducted in the above three areas in the summer and winter. The results showed that the summer average daylight rate (Rm-avg) in the middle area was 66.6%, while the Rm-avg in the other two areas was about 20%, with no significant difference. In the winter, the light environment in the southern area was slightly better, and the Rm-avg in the above three areas was 26.4%, 24.7% and 19.7%, respectively. On the whole, the relationship between the thermal environmental factors and the solar radiation intensity was consistent. Further, a 3D model of an OAVS was established using Autodesk Ecotect Analysis 2011, and the internal light environment was simulated. Compared with the measured values, the relative error was less than 10%, which verified the reliability of the OAVS model. Then, the model was used to reveal the temporal and spatial changes in the light environment of the OAVS. The simulation results showed that the daylighting rate in the summer from the ground to the height of the fig canopy inside the system was 20.7% to 61.5%. In the winter, the daylighting rate from the ground to the height of the fig canopy inside the system was 17.7% to 36.4%. The effectiveness of the OAVS in reducing the level of solar radiation intensity depended on the time of day and the angle of the sun. At the spatial scale, due to the strong consumption of light by photovoltaic panels, there was a strong horizontal and vertical light environment gradient inside the system. In conclusion, the photothermal environment research of an OAVS based on Autodesk Ecotect Analysis 2011 can not only provide a basis for agricultural production and structural design such as span, height and the laying density of PV panels, but also expand its application to regions with different latitudes and longitudes and specific climates.
Spatial Distribution of Leymus chinensis Is Not Determined by Its Ecological Stoichiometry
Authors: Wu Jinrui , Li Mengzhen , Yang Junyi , Yang Yong , Lv Shijie , Liu Hongmei , Tian Shichao , Yun Xiangjun , Yang Xia
Published: July 2023

Leymus chinensis is a widely distributed species of Eurasian grasslands that is well adapted to a range of environmental conditions. In this study, we measured soil and plant chemical elements to examine the basis of the ecological adaptations of L. chinensis to temperate steppe. We sampled leaves of L. chinensis and soil layers at different latitudes in temperate steppe for three consecutive years from 2012 to 2014. One-way analysis of variance and linear regression were used to analyze the variation in foliar nitrogen (N) and phosphorus (P) contents and the N:P ratio in L. chinensis. The correlations between the foliar N:P ratio and the variation in soil total nitrogen (TN) and total P contents with latitudinal gradient were measured to explore the homeostasis stoichiometry of foliar N and P in L. chinensis. With increasing latitude, both the foliar N and P contents of L. chinensis showed parabolic trends with significant negative correlations. The population of L. chinensis was limited by N, and the soil TN content showed a significant increasing trend with the increase in the foliar N:P ratio. The variation in the foliar N:P ratio of L. chinensis was determined through the variation in the soil N content. In different latitude intervals, the foliar N, P, and N:P ratio of L. chinensis showed absolute stability and strong internal stability, and the values of HN:P were high and similar in different latitudes. In conclusion, the population of L. chinensis in the temperate steppe is highly adaptable; and, thus, can maintain a dominant position in the variable environment and has a wide distribution range.
Secondary Metabolites, Other Prospective Substances, and Alternative Approaches That Could Promote Resistance against Phytophthora infestans
Authors: Dufková Hana , Greplová Marie , Hampejsová Romana , Kuzmenko Marharyta , Hausvater Ervín , Brzobohatý Břetislav , Černý Martin
Published: July 2023

Potato (Solanum tuberosum) is a valuable staple crop that provides nutrition for a large part of the human population around the world. However, the domestication process reduced its resistance to pests and pathogens. Phytophthora infestans, the causal agent of late blight disease, is the most destructive pathogen of potato plants. Considerable efforts have been made to develop late blight-resistant potato cultivars, but the success has been limited and present-day potato production requires the extensive use of fungicides. In this review, we summarize known sources of late blight resistance and obstacles in P. infestans control. We outline the problematic aspects of chemical treatment, the possible use of biological control, and available resources of natural resistance in wild Solanum accessions. We focus on prospective putative markers of resistance that are often overlooked in genome-centered studies, including secondary metabolites from alkaloid, phenylpropanoid, and terpenoid classes, lipids, proteins, and peptides. We discuss the suitability of these molecules for marker-assisted selection and the possibility of increasing the speed of conventional breeding of more resilient cultivars.
A Lightweight YOLOv8 Tomato Detection Algorithm Combining Feature Enhancement and Attention
Authors: Yang Guoliang , Wang Jixiang , Nie Ziling , Yang Hao , Yu Shuaiying
Published: July 2023

A tomato automatic detection method based on an improved YOLOv8s model is proposed to address the low automation level in tomato harvesting in agriculture. The proposed method provides technical support for the automatic harvesting and classification of tomatoes in agricultural production activities. The proposed method has three key components. Firstly, the depthwise separable convolution (DSConv) technique replaces the ordinary convolution, which reduces the computational complexity by generating a large number of feature maps with a small amount of calculation. Secondly, the dual-path attention gate module (DPAG) is designed to improve the model’s detection precision in complex environments by enhancing the network’s ability to distinguish between tomatoes and the background. Thirdly, the feature enhancement module (FEM) is added to highlight the target details, prevent the loss of effective features, and improve detection precision. We built, trained, and tested the tomato dataset, which included 3098 images and 3 classes. The proposed algorithm’s performance was evaluated by comparison with the SSD, faster R-CNN, YOLOv4, YOLOv5, and YOLOv7 algorithms. Precision, recall rate, and mAP (mean average precision) were used for evaluation. The test results show that the improved YOLOv8s network has a lower loss and 93.4% mAP on this dataset. This improvement is a 1.5% increase compared to before the improvement. The precision increased by 2%, and the recall rate increased by 0.8%. Moreover, the proposed algorithm significantly reduced the model size from 22 M to 16 M, while achieving a detection speed of 138.8 FPS, which satisfies the real-time detection requirement. The proposed method strikes a balance between model size and detection precision, enabling it to meet agriculture’s tomato detection requirements. The research model in this paper will provide technical support for a tomato picking robot to ensure the fast and accurate operation of the picking robot.
Effect of Fibroblast Growth Factor 10 and an Interacting Non-Coding RNA on Secondary Hair Follicle Dermal Papilla Cells in Cashmere Goats’ Follicle Development Assessed by Whole-Transcriptome Sequencing Technology
Authors: Gao Yuan , Song Weiguo , Hao Fei , Duo Lei , Zhe Xiaoshu , Gao Chunyan , Guo Xudong , Liu Dongjun
Published: July 2023

Cashmere, a keratinised product of secondary hair follicles (SHFs) in cashmere goats, holds an important place in international high-end textiles. However, research on the complex molecular and signal regulation during the development and growth of hair follicles (HFs), which is essential for the development of the cashmere industry, is limited. Moreover, increasing evidence indicates that non-coding RNAs (ncRNAs) participate in HF development. Herein, we systematically investigated a competing endogenous RNA (ceRNA) regulatory network mediated by circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in skin samples of cashmere goat embryos, using whole-transcriptome sequencing technology. We obtained 6468, 394, and 239 significantly differentially expressed mRNAs, circRNAs, and miRNAs, respectively. These identified RNAs were further used to construct a ceRNA regulatory network, mediated by circRNAs, for cashmere goats at a late stage of HF development. Among the molecular species identified, miR-184 and fibroblast growth factor (FGF) 10 exhibited competitive targeted interactions. In secondary HF dermal papilla cells (SHF-DPCs), miR-184 promotes proliferation, inhibits apoptosis, and alters the cell cycle via the competitive release of FGF10. This study reports that FGF10 and its interaction with ncRNAs significantly affect SHF-DPCs, providing a reference for research on the biology of HFs in cashmere goats and other mammals.
Seasonal Variation in Gut Microbiota of the Wild Daurian Ground Squirrel (Spermophilus dauricus): Metagenomic Insights into Seasonal Breeding
Authors: Song Fengcheng , Ma Shubao , Zhang Yujiao , Yang Xiaoying , Zhang Haolin , Han Yingying , Liu Yuning , Gao Fuli , Yuan Zhengrong
Published: July 2023

The Spermophilus dauricus, the wild Daurian ground squirrel, is known to exhibit seasonal breeding behavior. Although the importance of gut microbiota in animal digestion, metabolism, and immunity is well-established, the correlation between gut microbiota and seasonal breeding in this species remains inadequately explored. In the present study, using metagenomic sequencing technology, the compositions and functions of the gut microbiota of wild Daurian ground squirrels in different breeding seasons were explored. The dominant gut microbial phyla were Firmicutes and Bacteroidetes. The Firmicutes were predominant in the breeding season, whereas Bacteroidetes were predominant in the non-breeding season. At the genus level, Lactobacillus accumulated during the breeding season, whereas Odoribacter and Alistipes increased during the non-breeding season. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genome) annotations indicated that genes in gut samples were highly associated with metabolic functions. The differential expression gene analysis showed that genes related to the phosphotransferase system, cysteine, and methionine metabolism were highly expressed during the breeding season, whereas the non-breeding season upregulated genes were enriched in starch and sucrose metabolism and bacterial chemotaxis pathways. In conclusion, this study could provide a reference for investigating gut microbiota in seasonal breeding animals and offer new insight into gut microbial function.
Role of Macrophages in the Pathogenesis of Genotype VII Newcastle Disease Virus in Chickens
Authors: Ni Jie , Deng Jing , Chen Qing , Liao Tianxing , Hu Jiao , Chen Yu , Hu Shunlin , Hu Zenglei , Liu Xiufan
Published: July 2023

Long-term evolution of Newcastle disease virus (NDV) results in substantial alteration in viral pathogenesis. NDVs of genotype VII, a late genotype, show marked tropism to lymphoid tissues, especially to macrophages in chickens. However, the role of macrophages in the pathogenesis of genotype VII NDV is still unclear. Herein, NDV infectivity in macrophages and the role of macrophages in the pathogenesis of genotype VII NDV in chickens were investigated. We reported that NDV strains of genotype VII (JS5/05) and IV (Herts/33) can replicate in the adherent (predominantly macrophages) and non-adherent cells (predominantly lymphocytes) derived from chicken peripheral blood mononuclear cells (PBMCs), and significantly higher virus gene copy was detected in the adherent cells. In addition, JS5/05 had significantly higher infectivity in PBMC-derived adherent cells than Herts/33, correlating with its enhanced tropism to macrophages in the spleen of chickens. Interestingly, the depletion of 68% of macrophages exerted no significant impact on clinical signs, mortality and the systematic replication of JS5/05 in chickens, which may be associated with the contribution of non-depleted macrophages and other virus-supportive cells to virus replication. Macrophage depletion resulted in a marked exacerbation of tissue damage and apoptosis in the spleen caused by JS5/05. These findings indicated that macrophages play a critical role in alleviating tissue damage caused by genotype VII NDV in chickens. Our results unveiled new roles of macrophages in NDV pathogenesis in chickens.
Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent
Authors: Zheng Mingde , Liu Xikui , Meng Yu , Lin Xiao , Li Jiahui , Zhu Jianguo , Zhao Minmeng , Liu Long , Geng Tuoyu , Gong Daoqing , Zhang Jun
Published: July 2023

The mechanism of sex determination in chickens, especially the molecular mechanism of female ovarian development, has not yet been fully elucidated. Previous studies have shown that RSPO1, which is associated with ovarian development in mammals, might have a conserved role in chickens. In this study, we systematically investigated the spatiotemporal expression pattern of RSPO1 in various tissues, especially gonads, of male and female chicken embryos using qPCR and Western blotting, and we explored its correlation with the expression of key genes in the estrogen pathway using drug treatment or gene overexpression in vivo and in vitro. Our results reveal that RSPO1 was widely expressed in all examined tissues of chicken embryos, showing a female bias in gonadal tissues at both the mRNA and protein levels. Surprisingly, RSPO1 was not differentially expressed between male and female gonadal cells with fadrozole-induced estrogen pathway blockades, and furthermore, estradiol-induced estrogen stimulation altered the expression of RSPO1. In addition, overexpression of RSPO1 in gonadal cells induced the mRNA expression of its downstream target genes, Wnt family member 4 (WNT4) and Catenin beta 1 (CTNNB1), and that of estrogen receptor α (ERα), an estrogen pathway gene. In summary, this study provided new evidence for elucidating the role of RSPO1 in ovarian development in poultry.
Effects of Thymol and Carvacrol Eutectic on Growth Performance, Serum Biochemical Parameters, and Intestinal Health in Broiler Chickens
Authors: Li Lixuan , Chen Xiaochun , Zhang Keying , Tian Gang , Ding Xuemei , Bai Shiping , Zeng Qiufeng
Published: July 2023

This study aimed to evaluate the effect of diets supplementing with various levels of thymol and carvacrol eutectic (TCE) on growth performance, serum biochemical parameters, intestinal morphology, and the expression of intestinal nutrient absorption, barrier function- and inflammation-related genes in broiler chickens. A total of 640 one-day-old Arbor Acres male broilers with similar body weights were randomly divided into four groups (8 replicates/group, 20 broilers/replicate). Birds in the four experimental groups were fed a basal diet with TCE at 0, 30, 60, or 120 mg/kg. The results showed that the growth performance of birds during 22–42 d or 1–42 d, serum IgE and IgG content at 21 d of age, jejunal and ileal morphology, ileal MUC2, OCLN, and IL-10 mRNA expression were significantly increased compared with the control group (p < 0.05), and the ileal IL-6 mRNA expression quadratically decreased (p < 0.05) with increasing dietary TCE supplemented dosage, and its expression showed a linear downward trend (0.05 < p < 0.1). Meanwhile, compared with the other three groups, birds fed diets with 30 mg/kg TCE presented better (p < 0.05) growth performance, intestinal morphology, and function. These results indicated that the optimal supplementation amount of TCE in the broiler diets was 30 mg/kg.
A Large-Scale Study into Protist-Animal Interactions Based on Public Genomic Data Using DNA Barcodes
Authors: Xie Jiazheng , Tan Bowen , Zhang Yi
Published: July 2023

With the birth of next-generation sequencing (NGS) technology, genomic data in public databases have increased exponentially. Unfortunately, exogenous contamination or intracellular parasite sequences in assemblies could confuse genomic analysis. Meanwhile, they can provide a valuable resource for studies of host-microbe interactions. Here, we used a strategy based on DNA barcodes to scan protistan contamination in the GenBank WGS/TSA database. The results showed a total of 13,952 metazoan/animal assemblies in GenBank, where 17,036 contigs were found to be protistan contaminants in 1507 assemblies (10.8%), with even higher contamination rates in taxa of Cnidaria (150/281), Crustacea (237/480), and Mollusca (107/410). Taxonomic analysis of the protists derived from these contigs showed variations in abundance and evenness of protistan contamination across different metazoan taxa, reflecting host preferences of Apicomplexa, Ciliophora, Oomycota and Symbiodiniaceae for mammals and birds, Crustacea, insects, and Cnidaria, respectively. Finally, mitochondrial proteins COX1 and CYTB were predicted from these contigs, and the phylogenetic analysis corroborated the protistan origination and heterogeneous distribution of the contaminated contigs. Overall, in this study, we conducted a large-scale scan of protistan contaminant in genomic resources, and the protistan sequences detected will help uncover the protist diversity and relationships of these picoeukaryotes with Metazoa.
Evaluation of the Physical Characteristics and Chemical Properties of Black Soldier Fly (Hermetia illucens) Larvae as a Potential Protein Source for Poultry Feed
Authors: Pornsuwan Rattanakorn , Pootthachaya Padsakorn , Bunchalee Pasakorn , Hanboonsong Yupa , Cherdthong Anusorn , Tengjaroenkul Bundit , Boonkum Wuttigrai , Wongtangtintharn Sawitree
Published: July 2023

The aim of this study was to investigate and compare the effects of different drying methods on the physical and chemical properties of black soldier fly larvae (BSFL) to determine their potential as an alternative protein source in animal feed. The experimental design was a 2 × 3 factorial arrangement in a completely randomized design (BSFL type × drying method), with five replications. The influence of post-harvest procedures was studied, including the different BSFL types (non-defatted and defatted) and drying methods (parabola dome, hot air oven, and microwave). The results showed that the types of BSFL, drying methods, and their interaction significantly (p < 0.001) influenced the feed’s physical properties; these included the brightness of color (L* 29.74–54.07; a* 0.40–5.95; b* 9.04–25.57), medium bulk density (381.54–494.58 g/L), free flow with an angle of repose (41.30–45.40°), and small particle size. They significantly (p < 0.001) influenced the nutritive value of BSFL, which contained 42–59% crude protein, 7–14% crude fiber, 9–30% ether extract, and 5035–5861 kcal/kg of energy. Overall, both BSFL types and all the drying methods resulted in a slight variation in the proximate composition. However, a microwave and a hot-air oven were considered the most suitable methods for producing BSFL powder because of the high levels of nutrients retained and the improved physical parameters when compared to a parabola dome. This characterization of the physical and chemical composition of BSFL represents a preliminary methodology that could be used to initially preprocess larvae for use as an alternative protein source in animal feed and for other applications.
Handling and Training of Wild Animals: Evidence and Ethics-Based Approaches and Best Practices in the Modern Zoo
Authors: Brando Sabrina , Norman Max
Published: July 2023

There is an ethical responsibility to provide all animals living in human care with optimal and positive well-being. As animals living in zoos and aquariums frequently interact with their human caregivers as part of their daily care routines, it is both relevant and essential to consider the impact of these interactions on animal well-being. Allowing animals to have choice and control in multiple areas of their lives, such as by providing opportunities for them to voluntarily participate in their own care through, for example, positive reinforcement training, is an essential component of good animal well-being programs. This review aims to describe evidence-based approaches, ethics, and best practices in the handling and training of the many taxa held in zoos and aquariums worldwide, drawing from work in related animal care fields such as laboratories, farms, rescue, and sanctuaries. The importance of ongoing animal well-being assessments is discussed, with a particular focus on the need for continued review and refinement of processes and procedures pertaining to animal training and handling specifically. Review, enquiry, assessment, evaluation, and refinement will aim to dynamically support positive well-being for all animals.
Insights into the Response in Digestive Gland of Mytilus coruscus under Heat Stress Using TMT-Based Proteomics
Authors: Xu Lezhong , Wang Yuxia , Lin Shuangrui , Li Hongfei , Qi Pengzhi , Buttino Isabella , Wang Weifeng , Guo Baoying
Published: July 2023

Ocean warming can cause injury and death in mussels and is believed to be one of the main reasons for extensive die-offs of mussel populations worldwide. However, the biological processes by which mussels respond to heat stress are still unclear. In this study, we conducted an analysis of enzyme activity and TMT-labelled based proteomic in the digestive gland tissue of Mytilus coruscus after exposure to high temperatures. Our results showed that the activities of superoxide dismutase, acid phosphatase, lactate dehydrogenase, and cellular content of lysozyme were significantly changed in response to heat stress. Furthermore, many differentially expressed proteins involved in nutrient digestion and absorption, p53, MAPK, apoptosis, and energy metabolism were activated post-heat stress. These results suggest that M. coruscus can respond to heat stress through the antioxidant system, the immune system, and anaerobic respiration. Additionally, M. coruscus may use fat, leucine, and isoleucine to meet energy requirements under high temperature stress via the TCA cycle pathway. These findings provide a useful reference for further exploration of the response mechanism to heat stress in marine mollusks.
Guinea Pig Sperm Morphology and Fertility under Different Photoperiod
Authors: Quispe-Ccasa Hurley Abel , Briceño-Mendoza Yander M. , Cayo-Colca Ilse Silvia
Published: July 2023

Sperm morphology can predict the reproductive male fertilizing potential. This study aimed to determine the morphological and morphometric spermatozoa characteristics from guinea pigs subjected to different photoperiodic stimulation. Thirty F1 guinea pigs were randomly assigned to three photoperiodic treatments: FT1 (photoperiod with 10 Light/14 Dark LED light), FT2 (photoperiod with 10L/14D sunlight), and FT0 (room without direct light source). At 107 ± 9.8 days of age, sperm concentration and motility were higher in the FT0 and FT1 groups (p < 0.05); furthermore, there were no differences in nucleus length and ellipticity between the FT0 and FT1 groups, but the sperm of the FT1 group was higher in perimeter and nuclear area, while that of the FT0 group was higher in roughness, regularity, midpiece length, and tail (p < 0.01). Expanding acrosome (Type 2) was more frequent in the FT2 group, but there was variation in head measurements between all morphological categories. Pregnancy rate, calving age, and mating age were higher in the FT0 group; meanwhile, the FT1 group initiated successful matings earlier (p < 0.01). The FT0 group had a higher fertility rate, and the age of mating and first calving were earlier in the FT1 group than the FT0 group, but no pregnancies were reported for the FT2 group. Photoperiodic stimulation can increase the morphometric dimensions of guinea pig spermatozoa, favoring the reproductive characteristics, but sunlight could reduce their size due to heat stress.
The Effect of Ethyl Esters of Linseed Oil on the Changes in the Fatty Acid Profile of Hair Coat Sebum, Blood Serum and Erythrocyte Membranes in Healthy Dogs
Authors: Wyrostek Anna , Czyż Katarzyna , Sokoła-Wysoczańska Ewa , Patkowska-Sokoła Bożena , Bielas Wiesław
Published: July 2023

The aim of this study was to determine the effect of supplementation with ethyl esters of linseed oil on the fatty acid profile in hair sebum, blood serum and erythrocyte membranes in healthy dogs. The material for the study included hair and blood samples of adult beagle dogs. The experiment was performed in two periods: summer and winter. Each time it lasted 16 weeks. During the first 8 weeks, the dogs received a supplement, the amount of which was determined individually so that the ratio of α-linolenic acid (ALA) to linoleic acid (LA) together in the feed and supplement was 1:1. Hair coat and blood samples were collected on days 0, 56 and 112; i.e., before the start of supplementation, after 8 weeks of supplementation and 8 weeks after supplementation was completed. The study included a determination of the fatty acid profile with a particular emphasis on polyunsaturated fatty acids (PUFAs) and the ratio of omega-6 to omega-3 in hair sebum, blood serum and erythrocyte membranes. As a result of supplementation, a significant decrease in saturated acids and an increase in unsaturated acids was observed in hair sebum both in summer and winter and especially in omega-3 fatty acids; i.e., α-linolenic (ALA) and its derivatives eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The same relationships were observed in blood serum and in erythrocyte cell membranes in all the studied periods. Additionally, 8 weeks after the end of supplementation, the level of polyunsaturated fatty acids was still higher compared to the period before supplementation. Moreover, the supplementation resulted in a favorable decrease in the ratio of omega-6 to omega-3 acids in the tested samples, which persisted even after the withdrawal period.
Dynamic Analysis and Optimal Control of Fractional Order African Swine Fever Models with Media Coverage
Authors: Shi Ruiqing , Zhang Yihong , Wang Cuihong
Published: July 2023

African swine fever is a highly contagious virus that causes pig disease. Its onset process is short, but the mortality rate is as high as 100%. There are still no effective drugs that have been developed to treat African swine fever, and prevention and control measures are currently the best means to avoid infection in pig herds. In this paper, two fractional order mathematical models with media coverage are constructed to describe the transmission of African swine fever. The first model is a basic model with media coverage, and no control measures are considered. For this model, the reproduction number is obtained by using the next generation matrix method. Then, the sufficient conditions for the existence and stability of two equilibriums are obtained. Based on the first model, the second model is established incorporating two control measures. By using Pontryagin’s maximal principle, the optimal control solution is derived. After that, some numerical simulations are performed for the two models to verify the theoretical results. Both the qualitative analysis and numerical results indicate that timely media coverage combined with disinfection control measures is crucial to preventing the spread of disease.
Effect of Dam Body Conformations on Birth Traits of Calves in Chinese Holsteins
Authors: Yang Jiayu , Zhang Zhipeng , Lu Xubin , Yang Zhangping
Published: July 2023

The objective of this study was to explore the effect of dam body conformations on birth traits including stillbirth, dystocia, gestation length and birth weight of Chinese Holstein calves and to provide a reference for improving cow reproductive performance. We collected phenotype data on 20 conformation traits of Chinese Holstein cows and analyzed the impact of dam conformation trait linear scores on stillbirth, dystocia, gestation length and calf birth weight. The feet angle, set of rear legs, fore udder attachment and rear attachment height traits of the dairy cows significantly affected the risk of stillbirth. The risk of dystocia decreases with the increase in stature and pin width. The bone quality of dairy cows had a significant positive correlation with gestation length. There was a significant positive correlation between fore udder attachment and calf weight at birth. The birth weight of calves from cows with high body conformation traits was significantly higher than that of calves with a low composite score. These results suggest that improving the body conformation traits, especially the selection of mammary system and body shape total score, will be beneficial in improving the reproductive performance of dairy cows.
Prevalence of Enterococci and Vancomycin Resistance in the Throat of Non-Hospitalized Individuals Randomly Selected in Central Italy
Authors: Palmieri Annalisa , Martinelli Marcella , Pellati Agnese , Carinci Francesco , Lauritano Dorina , Arcuri Claudio , Baggi Luigi , Gatto Roberto , Scapoli Luca
Published: July 2023

Enterococci are commonly found in the environment and humans as a part of the normal microbiota. Among these, Enterococcus faecalis and Enterococcus faecium can convert into opportunistic pathogens, making them a major cause of nosocomial infections. The rapid diffusion of vancomycin-resistant strains and their impact on nosocomial settings is of considerable concern. Approximately one-third of the E. faecium infections in Italy are caused by vancomycin-resistant strains. This study explored the hypothesis that the oral cavity could represent a silent reservoir of virulent enterococci. A sample of 862 oral flora specimens collected from healthy human volunteers in Central Italy was investigated by real-time PCR to detect E. faecalis and E. faecium, as well as the genetic elements that most frequently determine vancomycin resistance. The prevalence of E. faecalis was 19%, a value that was not associated with alcohol consumption, tobacco smoking, or age of the subjects. Less frequently detected, with an overall prevalence of 0.7%, E. faecium was more common among people older than 49 years of age. The genes conferring vancomycin resistance were detected in only one sample. The results indicate that the oral cavity can be considered a reservoir of clinically relevant enterococci; however, our data suggest that healthy individuals rarely carry vancomycin-resistant strains.
Assessment of Antimicrobial Consumption in Multi-Field Hospitals with Pediatric Inpatients: Conventional vs. Novel Pediatric-Adjusted Methodologies
Authors: Rachina Svetlana , Belkova Yuliya , Kozlov Roman , Mladov Vladimir , Mishchenko Vladimir , Andreeva Alla , Domanskaya Olga , Portnjagina Ulyana , Dushina Anastasiia , Zainalabidova Khadizhat
Published: July 2023

Background: the objective of this study was to propose a methodology for the assessment of antimicrobial consumption (AMC) in pediatric inpatients and to estimate variances in consumption levels in multi-field hospitals with pediatric inpatients, calculated by means of the pediatric-adjusted methodology vs. the conventional methodology. Methods: the pediatric-adjusted methodology based on the conventional ATC/DDD method and children’s DDDs (cDDD) for antimicrobials were proposed and validated in a series of probabilistic sensitivity analyses of real clinical data extracted from the receipt notes of three multi-field hospitals. Differences in AMC in multi-field hospitals with pediatric inpatients, calculated by means of the proposed methodology vs. the conventional methodology, were assessed for a virtual cohort of inpatients, with the pediatric share increasing by 1%. Results: in children ≤12 years old, assessment by the standard methodology resulted in a 59% underestimation of AMC from the levels based on prescribed doses, vs. a 25% underestimation for the proposed methodology. In a mixed-age virtual population of inpatients, the underestimation of consumption levels rose to 321% for the ATC/DDD methodology compared to the proposed one. Conclusions: the proposed methodology demonstrated a higher accuracy of AMC estimates compared to the conventional one and can be considered for the quantification of antimicrobial utilization in pediatric institutions and multi-field hospitals with a substantial share of pediatric inpatients.
Antibiotic Treatment for Lower Respiratory Tract Infections in Primary Care: A Register-Based Study Examining the Role of Radiographic Imaging
Authors: Carlsson Sara , Hedin Katarina , Cronberg Olof , Moberg Anna
Published: July 2023

When imaging (i.e., chest-x-ray or computed tomography) is used to differentiate between acute bronchitis and pneumonia, many patients are being prescribed antibiotics despite the absence of radiographic pneumonia signs. This study of lower respiratory tract infections (LRTIs) with negative chest imaging compares cases where antibiotics were prescribed and not prescribed to find characteristics that could explain the prescription. Data were extracted from the regional electronic medical record system in Kronoberg County, Sweden, for patients aged 18–79 years diagnosed with acute bronchitis or pneumonia and who had any chest radiologic imaging between 2007–2014. Of 696 cases without evidence of pneumonia on imaging, 55% were prescribed antibiotics. Age, sex, and co-morbidity did not differ between those with or without antibiotics. The median level of C-reactive protein was low in both groups but differed significantly (21 vs. 10 mg/L; p < 0.001). Resident physicians prescribed antibiotics more frequently than interns or specialists (p < 0.001). It is unclear what features prompted the antibiotic prescribing in those with negative imaging indicating overuse of antibiotics for LRTIs.
Antibiotic, Heavy Metal, and Biocide Concentrations in a Wastewater Treatment Plant and Its Receiving Water Body Exceed PNEC Limits: Potential for Antimicrobial Resistance Selective Pressure
Authors: Chukwu Kelechi B. , Abafe Ovokeroye A. , Amoako Daniel G. , Essack Sabiha Y. , Abia Akebe L. K.
Published: July 2023

Although the rise in antimicrobial resistance has been attributed mainly to the extensive and indiscriminate use of antimicrobials such as antibiotics and biocides in humans, animals and on plants, studies investigating the impact of this use on water environments in Africa are minimal. This study quantified selected antibiotics, heavy metals, and biocides in an urban wastewater treatment plant (WWTP) and its receiving water body in Kwazulu-Natal, South Africa, in the context of the predicted no-effect concentrations (PNEC) for the selection of antimicrobial resistance (AMR). Water samples were collected from the WWTP effluent discharge point and upstream and downstream from this point. Heavy metals were identified and quantified using the United States Environmental Protection Agency (US EPA) method 200.7. Biocides and antibiotic residues were determined using validated ultra-high-performance liquid chromatography with tandem mass spectrometry-based methods. The overall highest mean antibiotic, metal and biocide concentrations were observed for sulfamethoxazole (286.180 µg/L), neodymium (Nd; 27.734 mg/L), and benzalkonium chloride (BAC 12) (7.805 µg/L), respectively. In decreasing order per sampling site, the pollutant concentrations were effluent > downstream > upstream. This implies that the WWTP significantly contributed to the observed pollution in the receiving water. Furthermore, most of the pollutants measured recorded values exceeding the recommended predicted no-effect concentration (PNEC) values, suggesting that the microbes in such water environments were at risk of developing resistance due to the selection pressure exerted by these antimicrobials. Further studies are required to establish such a relationship.
Discovery of Novel Myristic Acid Derivatives as N-Myristoyltransferase Inhibitors: Design, Synthesis, Analysis, Computational Studies and Antifungal Activity
Authors: Javid Saleem , Ather Hissana , Hani Umme , Siddiqua Ayesha , Asif Ansari Shaik Mohammad , Shanmugarajan Dhivya , Yogish Kumar Honnavalli , Arivuselvam Rajaguru , Purohit Madhusudan N. , Kumar B. R. Prashantha
Published: July 2023

In recent years, N-Myristoyltransferase (NMT) has been identified as a new target for the treatment of fungal infections. It is observed that at present, there are increased rates of morbidity and mortality due to fungal infections. Hence, a series of novel myristic acid derivatives were designed via molecular docking studies and ADMET studies by targeting NMT (N-Myristoyltransferase). The designed myristic acid derivatives were synthesized by converting myristic acid into myristoyl chloride and coupling it with aryl amines to yield corresponding myristic acid derivatives. The compounds were purified and characterized via FTIR, NMR and HRMS spectral analyses. In this study, we carried out a target NMT inhibition assay. In the NMT screening assay results, the compounds 3u, 3m and 3t showed better inhibition compared to the other myristic acid derivatives. In an in vitro antifungal evaluation, the myristic acid derivatives were assessed against Candida albicans and Aspergillus niger strains by determining their minimal inhibitory concentrations (MIC50). The compounds 3u, 3k, 3r and 3t displayed superior antifungal capabilities against Candida albicans, and the compounds 3u, 3m and 3r displayed superior antifungal capabilities against Aspergillus niger compared to the standard drug FLZ (fluconazole). Altogether, we identified a new series of antifungal agents.
The Influence of Exercise on Oxidative Stress after Spinal Cord Injury: A Narrative Review
Authors: Maugeri Grazia , Amato Alessandra , Sortino Martina , D′Agata Velia , Musumeci Giuseppe
Published: July 2023

Spinal cord injury (SCI) is an irreversible disease resulting in partial or total loss of sensory and motor function. The pathophysiology of SCI is characterized by an initial primary injury phase followed by a secondary phase in which reactive oxygen species (ROSs) and associated oxidative stress play hallmark roles. Physical exercise is an indispensable means of promoting psychophysical well-being and improving quality of life. It positively influences the neuromuscular, cardiovascular, respiratory, and immune systems. Moreover, exercise may provide a mechanism to regulate the variation and equilibrium between pro-oxidants and antioxidants. After a brief overview of spinal cord anatomy and the different types of spinal cord injury, the purpose of this review is to investigate the evidence regarding the effect of exercise on oxidative stress among individuals with SCI.