bluuebunny/crossref_metadata_2025 huggingface dataset:
Lithostratigraphy and Limestone Microfacies of the Jafnayn Formation (Paleocene to Early Eocene, Al-Khod, Sultanate of Oman): Deposition in a Restricted Lagoon with Intervals of Open Marine Conditions
Authors: Mattern Frank , Scharf Andreas , Al-Sayigh Abdul Razak , Al-Mamari Abdulaziz , Galluccio Laura , Al-Ghaiti Sundus , Frijia Gianluca , Consorti Lorenzo , Al-Saadi Maram , Al-Jabri Fatema
Published: December 2024

We studied the Jafnayn Formation’s lithostratigraphy, microfacies, depositional environment, and uncertain presence of the Paleocene/Eocene boundary and present the first detailed analysis of a 127-m-thick section using the standard microfacies (SMF)/facies zone (FZ) system. The formation is dominated by foraminiferal grainstones and packstones of SMF 18-FOR, followed by peloidal grainstones and packstones of SMF 16. Coral-red algae floatstones of SMF 8 occur sporadically. SMF 18-DASY appears only once. SMF 16 and 18-FOR suggest a restricted lagoon, whereas SMF 8 and 18-DASY reflect episodic open marine lagoonal conditions. The section consists of four lithostratigraphic units. Considerable detrital quartz near the base (Unit 1: 22%; sand, sandstone, conglomerate) and top (Unit 4: 40%; sand) coincides with a restricted lagoon affected by near-shore processes (base) and near-shore conditions (top). Open marine conditions show an elevated bioclast diversity in units 3 and 4. Unit 2 displays very thickly-bedded limestones of the restricted lagoon. The lagoon barriers likely consist of foraminifera and other allochems that were reworked from the lagoon to form shoals. Coral remains in units 3 and 4 suggest that coral build-ups in the upper part of the formation protected the lagoon as well. The early Eocene age of several basal Alveolina species in the lowermost 9 m of the studied section indicates that the section is incomplete, with the late Paleocene part and meters-thick basal yellow marl missing. The Paleocene/Eocene boundary is unexposed.
Use of Whooley Questions and GAD-2 Tools in Screening for Perinatal Mental Health: Current Expert Considerations
Authors: Grussu Pietro , Severo Melania , Jorizzo Gianfranco J. , Quatraro Rosa Maria
Published: December 2024

Background/Objectives: Mental health screening of perinatal women is recommended as an essential element of routine maternity health care. Knowledge of screening conditions in healthcare settings as indicated by NICE is limited. This scoping review examines current expert considerations in the scientific literature on the use of Whooley questions and GAD-2 tools in screening for women’s perinatal mental health. Methods: The search included literature in English published prior to April 2023. Searches in PsycINFO, PubMed, Scopus, Scilit, and Google Scholar used the terms “Whooley questions” and “GAD-2”. Results: A total of 9 articles on studies using both of these tools were included. During pregnancy, rapid screening instruments permit the identification of women at increased risk for postpartum depression. They also detect continuity between depressive or anxious symptoms during pregnancy and depressive symptoms after delivery. Effectiveness compares favorably to that of some lengthier screening instruments. Rapid self-report tools appear to be most suitable for initial screening. They are indicative only of perinatal mental health and have no diagnostic value. In the individual studies considered in this review, the reliability and sensitivity of the Whooley questions and GAD-2 are unclear. Because of their self-reporting nature, outcomes may be subject to recall bias. Conclusions: Future evaluation of the instruments’ performance over the perinatal period is needed.
Comprehensive Analysis of Volatile Organic Compounds and Their Impact on Apple Quality Following Some Essential Oil Treatments Against Botrytis cinerea
Authors: Karakus Sinem , Hatterman-Valenti Harlene , Sahin Muge , Kaya Ozkan
Published: December 2024

The susceptibility of apples to post-harvest decay by the fungus Botrytis cinerea has prompted innovative research into alternative preservation methods. In this regard, essential oils (EOs) have emerged as promising candidates due to their natural origin and potential antimicrobial properties. Investigating the biological significance of EO blends is crucial for understanding their potential antimicrobial mechanisms and evaluating their capacity to modulate metabolic responses that could inhibit post-harvest fungal decay in fruit tissues. This study delves into the intricate chemistry of apples when subjected to various EO treatments, shedding light on the profound changes in alcohols, esters, C6 compounds, terpenes, and volatile compounds. Based on our results, terpene concentrations exhibit significant variations with α-Pinene ranging from 13.4 µg L−1 in Fungus + Thymol + 1,8-Cineole treatment (Fun+Thy+Cin) to 28.7 µg L−1 in Fungus + Thymol + 1,8-Cineole + Eugenol treatment (Fun+Thy+Cin+Eug), and β-Pinene concentrations spanning 19.3 µg L−1 in Fungus + 1,8-Cineole + Eugenol treatment (Fun+Cin+Eug) to 45.5 µg L−1 in Fungus + Thymol + 1,8-Cineole + Eugenol treatment (Fun+Thy+Cin+Eug). Ester elaboration presents marked changes, with ethyl octanoate peaking at 715.7 µg L−1 in Fungus + Thymol + 1,8-Cineole + Eugenol treatment (Fun+Thy+Cin+Eug) and ethyl propionate reaching 152.9 µg L−1 in Fungus + Thymol treatment (Fun+Thy). The volatile compound dynamics also demonstrate significant variations, with hexanoic acid concentrations ranging from 0.1 to 0.2 among treatments and 3-Methylbutanal displaying concentrations from 0.8 to 6.4, with the highest concentration observed in the Control. The essential oil combination of Thymol, Eugenol, and 1,8-Cineol (Fun+Thy+Cin+Eug) had the most significant impact on the volatile compound content in the fruits. The findings from this study unveil the intricate responses of apple chemistry to various EO treatments. These insights hold promise for enhancing post-harvest apple preservation strategies through the modulation of EO treatments.
Comparative Analysis of Japanese Quince Juice Concentrate as a Substitute for Lemon Juice Concentrate: Functional Applications as a Sweetener, Acidifier, Stabilizer, and Flavoring Agent
Authors: Radenkovs Vitalijs , Krasnova Inta , Cinkmanis Ingmars , Juhnevica-Radenkova Karina , Rubauskis Edgars , Seglina Dalija
Published: December 2024

This research examined the viability of Japanese quince juice concentrate (JQJC) as an innovative alternative to lemon juice concentrate (LJC). Given the rising consumer demand for natural food ingredients, this study focused on a thorough analysis of the nutritional and functional characteristics of JQJC in comparison to LJC. The chemical analysis indicated that JQJC possesses a total soluble solids (TSS) content of 50.6 °Brix, with fructose and glucose, to a greater extent, being the primary contributors to its solids content. In contrast, LJC had a TSS of 39.8 °Brix and also contained glucose and fructose. Additionally, malic acid is a principal component of JQJC’s acidity, determined at 20.98 g 100 g−1 of fresh weight (FW), while LJC mostly contained citric acid at a concentration of 30.86 g 100 g−1 FW. Moreover, the ascorbic acid content quantified in JQJC was eight times greater than that observed in LJC. The assessment of antioxidant activity, utilizing the DPPH• and FRAP assays, indicated that JQJC exhibits scavenging activity nearly eleven times higher than that of LJC, suggesting its superior antioxidant capacity. The total phenolic content for JQJC was quantified at 2189.59 mg 100 g−1 FW, significantly (p < 0.05) exceeding the 262.80 mg 100 g−1 FW found in LJC. The analysis identified 16 individual phenolic compounds in JQJC, highlighting the dominance of epicatechin, chlorogenic, and protocatechuic acids with concentrations ranging from 0.16 to 50.63 mg 100 g−1 FW, contributing to a total individual phenolic content of 114.07 mg 100 g−1 FW. Conversely, LJC is characterized by substantial contributions from hesperidin, eriocitrin, and, to a lesser extent, quercetin-3-O-rutinoside, yielding a phenolic content of 109.65 mg 100 g−1 FW. This study presents strong evidence supporting the utilization of JQJC as a functional substitute for LJC across a variety of product categories, including beverages, jams, and other food items. The findings indicate that JQJC has the potential to enhance product development targeted at health-conscious consumers while optimizing the utilization of a relatively underexplored fruit crop.
Investigation of Oxidative-Stress Impact on Human Osteoblasts During Orthodontic Tooth Movement Using an In Vitro Tension Model
Authors: Hosseini Samira , Diegelmann Julia , Folwaczny Matthias , Sabbagh Hisham , Otto Sven , Kakoschke Tamara Katharina , Wichelhaus Andrea , Baumert Uwe , Janjic Rankovic Mila
Published: December 2024

In recent years, there has been a growing number of adult orthodontic patients with periodontal disease. The progression of periodontal disease is well-linked to oxidative stress (OS). Nevertheless, the impact of OS on orthodontic tooth movement (OTM) is not fully clarified. Therefore, we applied an OS in vitro-model utilizing H2O2 to study its effect on tension-induced mechanotransduction in human osteoblasts (hOBs). Experimental parameters were established based on cell viability and proliferation. Apoptosis detection was based on caspase-3/7 activity. Gene expression related to bone-remodeling (RUNX2, P2RX7, TNFRSF11B/OPG), inflammation (CXCL8/IL8, IL6, PTRGS2/COX2), autophagy (MAP1LC3A/LC3, BECN1), and apoptosis (CASP3, CASP8) was analyzed by RT-qPCR. IL6 and PGE2 secretion were determined by ELISA. Tension increased the expression of PTRGS2/COX2 in all groups, especially after stimulation with higher H2O2 concentration. This corresponds also to the measured PGE2 concentrations. CXCL8/IL8 was upregulated in all groups. Cells subjected to tension alone showed a general upregulation of osteogenic differentiation-related genes; however, pre-stimulation with OS did not induce significant changes especially towards downregulation. MAP1LC3A/LC3, BECN1 and CASP8 were generally upregulated in cells without OS pre-stimulation. Our results suggest that OS might have considerable impacts on cellular behavior during OTM.
A CsWRKY48 Gene from Tea Plants Intercropped with Chinese Chestnut Plays an Important Role in Resistance to Biotic and Abiotic Stresses
Authors: Wang Jianzhao , Gong Yikai , Li Meng , Bai Yan , Wu Tian
Published: December 2024

Tea plant (Camellia sinensis) is an important horticultural crop. The quality and productivity of tea plants is always threatened by various adverse environmental factors. Numerous studies have shown that intercropping tea plants with other plants can greatly improve the quality of their products. The intercropping system of Chinese chestnut (Castanea mollissima) and tea plants is an agricultural planting model in which the two species are grown on the same piece of land following a specific spacing and cultivation method. Based on a comparative transcriptome analysis between Chinese chestnut tea intercropped plantations and a pure tea plantation, it was found that the expression levels of the WRKY genes were significantly upregulated under the intercropping pattern. In this study, we cloned a candidate gene, CsWRKY48, and verified its functions in tobacco (Nicotiana tabacum) via heterologous transformation. The contents of protective enzyme activities and osmoregulatory substances were significantly increased, and the trichomes length and density were improved in the transgenic tobacco lines. This phenotype offered an enhanced resistance to both low temperatures and aphids for transgenic lines overexpressing CsWRKY48. Further analysis indicated that the CsWRKY48 transcription factor might interact with other regulators, such as CBF, ERF, MYC, and MYB, to enhance the resistance of tea plants to biotic and abiotic stresses. These findings not only confirm the elevated resistance of tea plants under intercropping, but also indicate a potential regulatory network mediated by the WRKY transcription factor.
Severity-Dependent Long-Term Post-Traumatic Changes in the Circulating Oxylipin Profile
Authors: Reinicke Madlen , Zheng Leyu , Rang Moujie , Fuchs Carolin , Weikert Juliane , Keß Annette , Kleber Christian , Ceglarek Uta , Osterhoff Georg , Aust Gabriela
Published: December 2024

Trauma causes the breakdown of membrane phospholipids and the subsequent degradation of the released polyunsaturated fatty acids (PUFAs) to partially bioactive oxylipins. Here, we screened for circulating PUFAs and oxylipins in patients (n = 34) differing from those of uninjured controls (n = 25) and analyzed their diagnostic potential. Patients were followed up for 1 to 240 h after minor/moderate, severe, and very severe injuries. Of the targeted oxylipins, 13 out of 80 (13/80) were detected in almost all patients and controls. Injury caused a long-term decrease in 9- and 13-hydroxyoctadecadienoic acids and in several dihydroxyeicosatetraenoic acids, the stable derivatives of bioactive anti-inflammatory epoxyeicosatrienoic acids, compared to controls. Frequently, these oxylipins correlated inversely to injury severity, days in the intensive care unit and hospital, and/or procalcitonin and pro-inflammatory cytokine levels 48 up to 240 h after trauma. Notably, 20/80 oxylipins were detected in some patients but not or less often in controls. Many of these oxylipins increased transiently immediately after injury. Their level is partly correlated with adverse clinical parameters at this early time point. The circulating oxylipidome was markedly affected by trauma. Several oxylipins showed injury-dependent alterations at different time points in the post-traumatic course.
Population Studies and Molecular Mechanisms of Human Radioadaptive Capabilities: Is It Time to Rethink Radiation Safety Standards?
Authors: Sosin Dmitry Vitalievich , Baranovskii Denis S. , Nechaev Denis Nikolaevich , Sosina Mariya Aleksandrovna , Shaposhnikov Alexander Vladimirovich , Trusov Georgy Aleksandrovich , Titova Anastasia Germanovna , Krasnikov Boris Fedorovich , Lomov Alexey Nikolaevich , Makarov Valentin Vladimirovich , Yudin Vladimir Sergeevich , Keskinov Anton Arturovich , Yudin Sergey Mihailovich , Klabukov Ilya Dmitrievich
Published: December 2024

The evolution of man on Earth took place under conditions of constant exposure to background ionizing radiation (IR). From this point of view, it would be reasonable to hypothesize the existence of adaptive mechanisms that enable the human organism to safely interact with IR at levels approximating long-term natural background levels. In some situations, the successful operation of molecular mechanisms of protection against IR is observed at values significantly exceeding the natural background level, for example, in cancer cells. In 15–25% of cancer patients, cancer cells develop a phenotype that is resistant to high doses of IR. While further investigations are warranted, the current evidence suggests a strong probability of observing positive health effects, including an increased lifespan, a reduced cancer risk, and a decreased incidence of congenital pathologies, precisely at low doses of ionizing radiation. This review offers arguments primarily based on a phenomenological approach and critically reconsidering existing methodologies for assessing the biological risks of IR to human health. Currently, in the most economically developed countries, there are radiation safety rules that interpret low-dose radiation as a clearly negative environmental factor. Nowadays, this approach may pose significant challenges to the advancement of radiomedicine and introduce complexities in the regulation of IR sources. The review also examines molecular mechanisms that may play a key role in the formation of the positive effects of low-dose IR on human radioadaptive capabilities.
Nanosilver–Biopolymer–Silica Composites: Preparation, and Structural and Adsorption Analysis with Evaluation of Antimicrobial Properties
Authors: Blachnio Magdalena , Zienkiewicz-Strzalka Malgorzata , Kutkowska Jolanta , Derylo-Marczewska Anna
Published: December 2024

In this article, we report on the research on the synthesis of composites based on a porous, highly ordered silica material modified by a metallic nanophase and chitosan biofilm. Due to the ordered pore system of the SBA-15 silica, this material proved to be a good carrier for both the biologically active nanophase (highly dispersed silver nanoparticles, AgNPs) and the adsorption active phase (chitosan). The antimicrobial susceptibility was determined against Gram-positive Staphylococcus aureus ATCC 25923, Gram-negative bacterial strains (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, and Pseudomonas aeruginosa ATCC 27853), and yeast Candida albicans ATCC 90028. The zones of microbial growth inhibition correlated with the content of silver nanoparticles deposited in the composites and were the largest for C. albicans (14–21 mm) and S. aureus (12–17 mm). The suitability of the composites for the purification of water and wastewater from anionic pollutants was evaluated based on kinetic and equilibrium adsorption studies for the dye Acid Red 88. The composite with the highest amount of the chitosan component showed the greatest adsorption capacity (am) of 0.57 mmol/g and the most effective kinetics with a rate constant (log k) and half-time (t0.5) of −0.21 and 1.62 min, respectively. Due to their great practical importance, AgNP–chitosan–silica composites can aspire to be classified as functional materials combining the environmental problem with microbiological activity.
Molecular Subgroups of HRD Positive Ovarian Cancer and Their Prognostic Significance
Authors: Kekeeva Tatiana , Dudina Irina , Andreeva Yulia , Tanas Alexander , Kalinkin Alexey , Musatova Victoria , Chernorubashkina Natalia , Khokhlova Svetlana , Tikhomirova Tatiana , Volkonsky Mikhail , Kutsev Sergey , Zaletaev Dmitry , Strelnikov Vladimir
Published: December 2024

Homologous recombination repair deficiency (HRD) is involved in the development of high-grade serous ovarian carcinoma (HGSOC) and its elevated sensitivity to platinum-based chemotherapy. To investigate the heterogeneity of the HRD-positive HGSOC we evaluated the HRD status, including BRCA mutations, genomic scar score, and methylation status of BRCA1/2 genes in 352 HGSOC specimens. We then divided the HRD-positive cohort into three molecular subgroups, the BRCA mutation cohort (BRCA+), BRCA1 methylation cohort (Meth+), and the rest of the HRD+ cohort (HRD+BRCA-Meth-), and evaluated their first-line chemotherapy response, benefit from olaparib, and progression-free survival (PFS). HRD-positive status was detected in 65% (228/352) of samples. The first group, BRCA+, accounted for 45% (102/228) of HRD positive cases and showed the best outcome in platinum therapy (ORR 96%), the highest olaparib benefit (p = 0.006) and the highest median PFS (46 months). The frequency of the second cohort, Meth+, among HRD-positive patients was 23% (52/228). Patients with Meth+ HGSOC showed a significantly poorer outcome, with a median PFS of 19 months, a significantly lower ORR to platinum therapy (84%) and a modest, but not significant, benefit from olaparib maintenance. The third HRD+BRCA-Meth- group accounted for 32% (74/228) of HRD-positive patients and showed an ORR to platinum therapy similar to that of the BRCA+ group (90%), a higher, but not statistically significant, benefit from olaparib and a median PFS of 23 months. In conclusion, Meth+ subgroup had poor outcomes in terms of chemotherapy response, olaparib benefit, and PFS compared to the other HRD+ subgroups, requiring a more thorough follow-up.
CYP9Q1 Modulates Dopamine to Increase Sugar Responsiveness in Honeybees (Apis mellifera)
Authors: Xu Xue-Ling , Geng Long , Zeng Zhao-Yang , Wu Zun , Li Lin-Feng , Tang Shao-Han , Wang Zi-Jing , Shi Han-Hui , Li Zhi-Guo , Nie Hong-Yi , Su Song-Kun
Published: December 2024

The appetite of honeybees for food is crucial to their survival and reproduction, as they sustain their entire colony by collecting pollen and nectar for nutrients. Dopamine, an important neurotransmitter, regulates appetite and satiety. However, how dopamine regulates honeybee foraging behavior remains unexplored. In this study, we investigated dopamine expression in 23-day-old Apis mellifera under different food-wanting conditions and identified differentially expressed genes (DEGs) in the brains of honeybees using RNA sequencing technology. We showed that dopamine levels in honeybees starved for 2 h were higher than those sated after 2 h of starvation. RNA-seq results revealed there were differences in the expression of cytochrome P450-dependent monooxygenase (CYP9Q1) in honeybees, which regulated the sucrose sensitivity of honeybees under different intake states. Furthermore, CYP9Q1 targeted the expression of the insulin receptor substrate (IRS) to promote dopamine synthesis. Our findings emphasize the relationship between dopamine and honeybees’ desire for food at the molecular level, providing a reference for further exploring the mechanism of food wanting.
Influence of Composition on the Patterns of Electrokinetic Potential of Thermosensitive N-(Isopropyl)Acrylamide Derivatives with Poly(Ethylene Glycol) Dimethacrylate and N-(2-Hydroxyethyl)Acrylamide
Authors: Gasztych Monika , Malamis-Stanowska Aleksandra , Trafalski Mateusz , Musiał Witold
Published: December 2024

The synthesis of poly(N-isopropyl acrylamide) (pNIPA)-based polymers via the surfactant-free precipitation polymerization (SFPP) method produced thermosensitive nanospheres with a range of distinctive physicochemical properties. Nano- and microparticles were generated using various initiators, significantly influencing particle characteristics, including the hydrodynamic diameter (DH), which varied from 87.7 nm to 1618.1 nm. Initiators, such as potassium persulfate and 2,2′-azobis(2-methylpropionamidine) dihydrochloride, conferred anionic and cationic functionalities, respectively, impacting the electrokinetic potential (EP) of the particles. Notably, certain particles with cationic initiators exhibited negative EP values at 18 °C, attributed to residual initiator components that affected the surface charge distribution. The presence of hydrophilic N-(2-hydroxyethyl)acrylamide (HEAA) segments also influenced solubility and phase transition behaviors, with critical dependencies on the HEAA/NIPA (N-isopropyl acrylamide) molar ratios. EP measurements taken at 18 °C and 42 °C revealed substantial differences, primarily governed by the initiator type and polymer composition. Observed variations in particle stability and size were associated with the choice of crosslinking agents and comonomer content, which affected both DH and EP in distinct ways. This study provides insights into key factors influencing colloidal stability and electrostatic interactions within thermosensitive polymer systems, underscoring their potential applications in biomedical and industrial fields.
Targeting Cancer: Microenvironment and Immunotherapy Innovations
Authors: Padzińska-Pruszyńska Irena Barbara , Taciak Bartłomiej , Kiraga Łukasz , Smolarska Anna , Górczak Małgorzata , Kucharzewska Paulina , Kubiak Małgorzata , Szeliga Jacek , Matejuk Agata , Król Magdalena
Published: December 2024

In 2024, the United States was projected to experience 2 million new cancer diagnoses and approximately 611,720 cancer-related deaths, reflecting a broader global trend in which cancer cases are anticipated to exceed 35 million by 2050. This increasing burden highlights ongoing challenges in cancer treatment despite significant advances that have reduced cancer mortality by 31% since 1991. Key obstacles include the disease’s inherent heterogeneity and complexity, such as treatment resistance, cancer stem cells, and the multifaceted tumor microenvironment (TME). The TME—comprising various tumor and immune cells, blood vessels, and biochemical factors—plays a crucial role in tumor growth and resistance to therapies. Recent innovations in cancer treatment, particularly in the field of immuno-oncology, have leveraged insights into TME interactions. An emerging example is the FDA-approved therapy using tumor-infiltrating lymphocytes (TILs), demonstrating the potential of cell-based approaches in solid tumors. However, TIL therapy is just one of many strategies being explored. This review provides a comprehensive overview of the emerging field of immuno-oncology, focusing on how novel therapies targeting or harnessing components of the TME could enhance treatment efficacy and address persistent challenges in cancer care.
General Physicochemical Parameters, Phenolic Composition, and Varietal Aromatic Potential of Three Red Vitis vinifera Varieties (“Merlot”, Syrah”, and “Saborinho”) Cultivated on Pico Island—Azores Archipelago
Authors: Jordão António M. , Correia Ana C. , Martins Bárbara , Romão Ana , Oliveira Bruno
Published: December 2024

Pico Island is one of the islands of the Azores archipelago located in the North Atlantic Ocean, where there are very specific conditions for vine cultivation. In this context, there is scarce knowledge related to grape ripening under these conditions. Thus, the aim of this study was to evaluate several physicochemical parameters, the phenolic composition, antioxidant capacity, and varietal aromatic potential, of the “Merlot”, Syrah”, and “Saborinho” grape varieties cultivated on Pico Island over three vintages. The outcomes obtained demonstrated that “Merlot” grapes showed a tendency for significantly higher values of estimated alcohol degree, total phenols, flavonoid and non-flavonoid phenols, total anthocyanins, color intensity, and antioxidant capacity over the three vintages. In addition, for individual anthocyanins, “Merlot” and “Syrah” grapes showed a predominance of acetyl-anthocyanins in relation to p-coumaroylated forms, while for “Saborinho” grapes, an oppositive tendency was observed. For varietal aromatic potential, only in the 2021 vintage was it possible to detect significantly different values between the three grape varieties studied. In this case, “Merlot” and “Syrah” grapes showed the significantly highest values. Considering all parameters analyzed, the results obtained for the “Merlot” grape variety seem to show a better adaptation of this variety to the conditions of Pico Island than the remaining two varieties studied.
Understanding User Behavior for Enhancing Cybersecurity Training with Immersive Gamified Platforms
Authors: Donekal Chandrashekar Nikitha , Lee Anthony , Azab Mohamed , Gracanin Denis
Published: December 2024

In modern digital infrastructure, cyber systems are foundational, making resilience against sophisticated attacks essential. Traditional cybersecurity defenses primarily address technical vulnerabilities; however, the human element, particularly decision-making during cyber attacks, adds complexities that current behavioral studies fail to capture adequately. Existing approaches, including theoretical models, game theory, and simulators, rely on retrospective data and static scenarios. These methods often miss the real-time, context-specific nature of user responses during cyber threats. To address these limitations, this work introduces a framework that combines Extended Reality (XR) and Generative Artificial Intelligence (Gen-AI) within a gamified platform. This framework enables continuous, high-fidelity data collection on user behavior in dynamic attack scenarios. It includes three core modules: the Player Behavior Module (PBM), Gamification Module (GM), and Simulation Module (SM). Together, these modules create an immersive, responsive environment for studying user interactions. A case study in a simulated critical infrastructure environment demonstrates the framework’s effectiveness in capturing realistic user behaviors under cyber attack, with potential applications for improving response strategies and resilience across critical sectors. This work lays the foundation for adaptive cybersecurity training and user-centered development across critical infrastructure.
Traversable Ledger for Responsible Data Sharing and Access Control in Health Research
Authors: Bose Sunanda , Marijan Dusica
Published: December 2024

Healthcare institutions and health registries often store patients’ health data. In order to ensure privacy, sensitive medical information is stored separately from the identifying information of the patient. Generally, institutions anonymize medical information while sharing it for external use. However, internal users may also use it for identifying inaccuracies or missing information. Even though internal users may be legally permitted to access sensitive medical information, such access may lead to the identification of the patient, which can be vulnerable to patient privacy. Ensuring the accountability and responsibility of the internal users may lead to tractability in case of adversarial access with malicious intentions. Therefore, a secure system must be developed for the storage and retrieval of health data. To this end, in this paper, we propose a ledger-based system that cryptographically ensures that all access to health data must be logged into a ledger. Nevertheless, the ledger entries must be protected against adversarial access, too. At the same time, the ledger must be traversable by the patients as well as internal users. To address these needs, we propose techniques for the construction of a ledger to permit both internal users and patients to securely traverse and view only the entries to which they are linked.
Age-Stage, Two-Sex Life Tables of Megalurothrips usitatus (Bagnall) and Frankliniella intonsa (Trybom) on Different Bean Pods Under Laboratory Conditions: Implications for Their Competitive Interactions
Authors: Li Mengni , Peng Zhengke , Guo Chaosong , Xiao Yong , Yin Fei , Yuan Haibin , Li Zhenyu , Zalucki Myron P.
Published: December 2024

As two major pests of cowpea in South China, bean flower thrips [Megalurothrips usitatus (Bagnall)] and flower thrips [Frankliniella intonsa (Trybom)] always occur on the same plant. In this study, the two-sex life table parameters of these two species were investigated on three bean pods: cowpea (Vigna unguiculata L. var. Chunqiu Wujiadou), snap bean (Phaseolus vulgaris L. var. Yonglong 3), and green bean (Phaseolus vulgaris L. var. Linghangzhe) using standard cut bean protocols. Longevity of female adult M. usitatus showed no significant difference on cowpea and snap bean, but was shortest on green bean, with the highest fecundity on snap bean, followed by cowpea and green bean. Longevity and fecundity of female adult F. intonsa were both highest on cowpea, followed by snap bean and green bean. On snap bean, the population parameters (R0, GRR, T) of M. usitatus were higher than F. intonsa, whereas r and λ were not different, suggesting that M. usitatus were more competitive on snap bean. On cowpea, the population parameters (R0, GRR, r, λ) of F. intonsa were higher than M. usitatus, suggesting a competitive advantage of F. intonsa on cowpea. To better mimic field conditions, we investigated the survival and fecundity of adult M. usitatus under two distinct scenarios: when they could feed directly on cut surfaces of cowpeas (standard lab protocol), and when they could not. Adult survival of M. usitatus is not significantly influenced by whether or not they fed directly on cut surfaces of cowpeas. However, a notable decrease in fecundity was observed in M. usitatus that fed on surface-covered cut pods. This suggested that direct feeding on cut surfaces of cowpea did have an impact on thrips’ fecundity, offering fresh perspectives for future research into the growth and development of thrips in a laboratory. Our study suggests that experiments involving cut surfaces may be misleading, and further investigations are ongoing to address these concerns.
Trends in Pediatric Neck Injuries During Sporting Activities
Authors: Lu Victor M. , Ramchandran Subaraman , Errico Thomas , George Stephen
Published: December 2024

Background: Although neck injuries secondary to sporting activities in the pediatric demographic presenting to the emergency department (ED) are common, predictors of needing higher-level care (HLC) outside immediate treatment and release are not clear. The aim of this study was to describe how these neck injuries present in the United States (US) and identify predictors of HLC. Methods: We interrogated the US National Electronic Injury Surveillance System (NEISS) database for presentations to the ED of pediatric patients (aged 6–18 years old) whose primary complaint was neck injury in the setting of sport between 2014 and 2023. Statistical analysis utilized weighted estimates to evaluate incidence and then univariate and multivariate regression analyses were carried out to identify parameters associated with HLC for these patients. Results: Overall, a national weighted total of 360,885 ED presentations were estimated to have occurred in the last decade without any statistical change over the years. The mean age was 12.7 years, with more males (60.0%) than females (40.0%). The most common race observed was Black (50.6%), and these injuries most commonly occurred at a sporting complex (62.9%). Neck strains (59.9%) were the most common diagnosis seen across all presentations, followed by neck pain (29.1%), neck contusions (including abrasion) (6.4%), neck fractures (1.7%) and neck lacerations (0.8%). Ultimately, the majority of presentations were treated and released from the ED (95.5%). There were 4.5% of presentations, however, that did require HLC. Older age (OR 1.07, p = 0.004), male gender (OR 1.51, p = 0.002), involvement of other body part(s) (OR 1.45, p = 0.007) and non-strain neck injuries (OR 11.8, p < 0.001) were all independent, statistically significant predictors of HLC. Football (18.6%) was the most common sport associated with these presentations overall, but this was driven mostly by male cases. For females, the most common sport associated with these presentations was cheerleading (12.3%). Conclusions: In the last decade, neck injuries secondary to sporting activities in the pediatric demographic presenting to the ED have remained consistent. We have identified a unique set of predictors for presentations requiring HLC. These findings can be used in tandem with the findings that there are a number of gender-specific sports that drive these presentations to develop more sensitive and specific protocols for both primary prevention and ED triaging.
Association Between Postpartum Depression and Personality Traits Among Japanese Postpartum Mothers and Fathers
Authors: Haku Ayana , Kaneko Hitoshi , Kawahito Junko
Published: December 2024

Background/Objectives: Although numerous investigations have been conducted on postpartum depression, studies on the association between postpartum depression and personality traits of mothers and fathers are lacking. This study aimed to examine the association between postpartum depression and the Big Five personality models among Japanese mothers and fathers at one-month health check-ups. Methods: The participants were 82 couples, and they responded to the Edinburgh Postnatal Depression Scale (EPDS), the Japanese version of the Ten-Item Personality Inventory, and the Quality of Marriage Index (QMI). We examined the correlations among variables and analyzed the data using structural equation modeling (SEM). Results: Maternal neuroticism was significantly associated with maternal depression (β = 0.50, p < 0.001), and maternal extraversion was significantly associated with paternal depression (β = −0.64, p < 0.001). In addition, we found that maternal postpartum depression was associated with maternal marital satisfaction (r = −0.29, p = 0.037); however, this association disappeared in SEM. Conclusions: Our findings suggest that health practitioners should pay attention to depression and personality traits in both postpartum mothers and fathers. Moreover, we should consider the different associations between depression and personality in parents when initiating interventions.
Pilot Study on the Relationship Between Different Lower Limb Raising Velocities and Trunk Muscle Contraction in Active Straight Leg Raise
Authors: Yoshikawa Kohei , Kida Noriyuki , Jiroumaru Takumi , Murata Yuta , Noguchi Shinichi
Published: December 2024

Background/Objectives: The active straight leg raise requires intricate coordination between the hip, knee, pelvis, and spine. Despite its complexity, limited research has explored the relationship between lower limb raising velocity and trunk muscle motor control during an active straight leg raise in healthy individuals. This study aimed to explore the potential effects of increased lower limb raising velocity on core muscle contractions during active straight leg raises. Methods: Six healthy adult men (mean age: 24.5 ± 2.5 years) participated in this study. Electromyography signals were recorded using surface electrodes placed on the rectus abdominis, external oblique, and internal oblique/transverse abdominis muscles. The participants performed active straight leg raises at three different velocities: 3 s, 2 s, and as fast as possible (max). The electromyography data were analyzed from 250 ms before to 1000 ms after movement initiation, with muscle activity expressed as a percentage of the maximal voluntary isometric contraction. Statistical analyses were conducted using non-parametric tests, including the Friedman test for overall differences, followed by pairwise Wilcoxon signed-rank tests with Bonferroni correction for multiple comparisons (p < 0.05). Results: During the 250 ms before movement initiation, the internal oblique/transverse abdominis, external oblique, and rectus abdominis muscles showed greater activity in the max condition compared to the 3 s and 2 s conditions (Friedman test, p < 0.05), but no significant differences were found in pairwise comparisons (Wilcoxon test, p > 0.05). Similarly, during the 500 ms after movement initiation, internal oblique/transverse abdominis activity was higher in the max condition, with no significant pairwise differences observed. Conclusions: Faster lower limb raising velocities during active straight leg raise may enhance core stability by activating anticipatory and sustained internal oblique/transverse abdominis, external oblique, and rectus abdominis activity on the raised limb side. Training to promote this activation could improve dynamic stability in rapid or asymmetric movements.
New Strategies to Combat Human Fungal Infections
Authors: Santos André Luis Souza dos , Branquinha Marta Helena
Published: December 2024

Over the past few decades, numerous reports have highlighted the significant rise in fungal infections worldwide, contributing to considerable morbidity, mortality, and escalating healthcare costs [...]
Diversity of Lignicolous Freshwater Fungi from Yuanjiang River in Yunnan (China), with the Description of Four New Species
Authors: Zhang Liang , Bao Dan-Feng , Shen Hong-Wei , Luo Zong-Long
Published: December 2024

Yuanjiang River (Red River) is one of the six major water systems in Yunnan Province, which originates from western Yunnan Province. This river system features numerous tributaries, complex terrain, and abundant natural resources. During the investigation on the diversity of lignicolous freshwater fungi in the Yuanjiang River, nine species were collected and identified, five belonging to Dothideomycetes and four to Sordariomycetes. Based on morphology and multigene phylogenetic analyses, four species, namely, Aquadictyospora aquatica, Dictyosporium fluminicola, Myrmecridium submersum, and Neomyrmecridium fusiforme, are described as new species. Dictyocheirospora aquadulcis is reported as a new national record, and Myrmecridium hydei is reported as a new habitat record. Dictyocheirospora rotunda, Halobyssothecium aquifusiforme, and Pseudohalonectria lutea were known earlier from freshwater habitats, but we described them in detail in this paper. This study contributes significantly to the understanding of the diversity of lignicolous freshwater fungi in southwestern China.
Whole-Genome Sequencing and Genome Annotation of Pathogenic Elsinoë batatas Causing Stem and Foliage Scab Disease in Sweet Potato
Authors: Xu Yuan , Liu Yuqing , Wang Yihan , Liu Yi , Zhu Guopeng
Published: December 2024

A pathogen strain responsible for sweet potato stem and foliage scab disease was isolated from sweet potato stems. Through a phylogenetic analysis based on the rDNA internal transcribed spacer (ITS) region, combined with morphological methods, the isolated strain was identified as Elsinoë batatas. To comprehensively analyze the pathogenicity of the isolated strain from a genetic perspective, the whole-genome sequencing of E. batatas HD-1 was performed using both the PacBio and Illumina platforms. The genome of E. batatas HD-1 is about 26.31 Mb long in 167 scaffolds, with a GC content of 50.81%, and 7898 protein-coding genes, 131 non-coding RNAs, and 1954 interspersed repetitive sequences were predicted. Functional annotation revealed that 408 genes encode virulence factors involved in plant disease (DFVF—Plant). Notably, twenty-eight of these virulence genes encode secretory carbohydrate-active enzymes (CAZymes), including two endo-1,4-β-xylanase genes and seven cutinase genes, which suggested that endo-1,4-β-xylanase and cutinase play a vital role in the pathogenicity of E. batatas HD-1 within sweet potato. In total, twelve effectors were identified, including five LysM effectors and two CDIP effectors, suggesting that LysM and CDIP effectors play significant roles in the interaction between E. batatas HD-1 and sweet potato. Additionally, our analysis of biosynthetic gene clusters (BGCs) showed that two gene clusters are involved in melanin and choline metabolism. This study enriches the genomic resources of E. batatas and provides a theoretical foundation for future investigations into the pathogenic mechanisms of its infection in sweet potatoes, as well as potential targets for disease control.
Higher Education Loan Schemes Across the Globe: A Systematic Review on the Utility Derived and Burden Associated with Educational Debt
Authors: Frank Daniel , Bhandary Rakshith , Prabhu Sudhir K.
Published: December 2024

Education is considered an investment in human capital that is gained at the cost of knowledge acquisition. This cost is borne by the beneficiary along with subsidy provided by the government, if any, that is mainly collected through tax revenues. This article aims to systematically review the utility derived and the burden experienced with educational debt borrowers across the globe as per the three types of educational loan schemes present across the globe. This study follows the PRISMA guidelines for review selection, and 47 articles published between 1994 and 2024 were included for the final review. The study results reveal that education improves the quality of life; an educational debt servicing to income ratio above 8% is considered as a financial burden. Also, the results reveal that material benefits are high after education along with an increase in the psychological burden because of repayment concerns. This study highlights the need to move towards designing a flexible repayment system in the education loan scheme based on the income contingent schemes adopted in many countries. Income contingent schemes reduce the repayment burden of the borrowers but the return to the lender is limited to the income of the borrower, and mortgage-based schemes are associated with high repayment burden. Therefore, a dynamic scheme will fix the problems associated with the repayment burden by creating a dynamic link between the benefits received and the contributions made by the borrower.
Physiotherapy Intervention in the Treatment of Venous Ulcers: Results from a Delphi Panel
Authors: Medeiros Sabrina , Rodrigues Alexandre , Costa Rui
Published: December 2024

Background/Objectives: Venous ulcers are the most common type of ulcerated wounds in the lower limbs and result from chronic venous insufficiency. The treatment of venous ulcers is multidisciplinary, with physiotherapy intervention serving as an adjuvant therapy in managing these wounds. This study investigated physiotherapeutic interventions for the management of venous ulcers. Methods: This was an exploratory and descriptive study using the Delphi method. The panel comprised 12 experts in wound care, including 25.0% physicians, 41.7% nurses, and 33.3% physiotherapists. Two rounds of analysis were conducted. A quantitative analysis was performed to assess the level of agreement in responses, while qualitative analysis was applied to the experts’ comments. Results: In the first round, consensus varied between 80% and 100%, and in the second round, it ranged from 83.3% to 100%. In the second round, all interventions obtained at least 80% consensus. The interventions included the use of compression therapy and therapeutic exercise: resistance training (2–3 sets of 10–25 repetitions, 3 times/day, for at least 12 weeks), aerobic exercise (at least 30 min, 3 times/week, for 12 weeks), stretching, balance training, and the use of a vibrating platform. Conclusions: Physiotherapy may be beneficial as an adjuvant therapy alongside specific local treatments for venous ulcers. Therapeutic exercise and compression therapy are commonly utilized interventions that could support overall treatment outcomes.
Pilot Study on the Effects of a Cosmetic Serum Containing Niacinamide, Postbiotics and Peptides on Facial Skin in Healthy Participants: A Randomized Controlled Trial
Authors: Rusic Doris , Ivic Magdalena , Slugan Ana , Leskur Dario , Modun Darko , Durdov Toni , Vukovic Dubravka , Bukic Josipa , Bozic Josko , Seselja Perisin Ana
Published: December 2024

The literature describes niacinamide, but also postbiotics and peptides, as ingredients that improve skin barrier function, but also affect melanin and sebum levels in individuals. However, the combined effects of these ingredients in a single cosmetic serum have not been sufficiently investigated. Therefore, the aim of this randomized controlled study, conducted at the University of Split School of Medicine (Croatia), was to evaluate the effects of cosmetic products containing these active ingredients. This study was registered with ClinicalTrials.gov (NCT06189105) and has been completed. Primary outcomes were the levels of trans epidermal water loss, skin hydration, erythema, melanin, and sebum, all measured in 25 healthy Caucasian participants. Significant differences between hydration levels were observed at week 4 (61.0 ± 11.2 vs. 68.6 ± 13.3 AU, control and intervention). Moreover, a significant decrease in erythema values from the first to last measurement in the intervention group was observed, (379.9 ± 106.8 vs. 333.6 ± 73.5 AU, baseline values and week 4, intervention group). Interestingly, both the increase in skin hydration levels and the decrease in skin erythema after niacinamide serum application were significant in study participants who did not use sun protection products. It is well known that ultraviolet radiation has detrimental effects on human skin, and our results suggest that niacinamide could help counteract these effects.