bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel PARP Inhibitor DDPF-20 Induces DNA Damage and Inhibits Angiogenesis
through the PI3K/Akt/VEGF Pathway
Authors: Wang Tian , Zhang Dong , Guo Chuanlong , Zhu Wenyong
Published: February 2022

Background:

Poly (ADP-ribose) polymerase (PARP) plays a key role in DNA damage repair. A novel
compound (E)-N'-(2,3-dibromo-4,5-dihydroxyphenyl)-N-(phenylcarbamothioyl)formimidamide (DDPF-20) with excellent
PARP inhibitory activity was synthesized.

Objective:

In this study, we aimed to clarify the mechanism of the novel PARP inhibitor DDPF-20 against lung cancer
by inducing DNA damage and inhibiting angiogenesis.

Method:

The cytotoxic effect of DDPF-20 on the A549 cell line was determined with an MTT assay. Cell cycle and
apoptosis were determined by a flow cytometer. Moreover, the γH2AX foci were detected by immunofluorescence.
Capillary-like tube formation assay and chick chorioallantoic membrane (CAM) assay were used to detect the angiogenesis
inhibitory effect of DDPF-20. The expressions of related proteins were detected by western blot. The anticancer
activity of DDPF-20 in vivo was also detected.

Results:

With an IC50 value of 52.42 ± 15.13 nM, DDPF-20 inhibited the proliferation, induced G2/M cycle arrest, and
induced apoptosis of human lung cancer A549 cells. Further research showed that DDPF-20 induced DNA doublestrand
breaks (DSBs). Interestingly, DDPF-20 inhibited the tube formation of HUVEC cells, as well as inhibited the
neovascularization of CAM, proving the angiogenesis inhibitory ability of DDPF-20. Mechanism studies proved that
DDPF-20 inhibited the PI3K/Akt/VEGF signaling pathway. In an in vivo study, DDPF-20 inhibited tumor growth of an
A549 xenograft. Analysis of the molecular mechanism underlying this effect revealed that the PI3K/Akt/VEGF pathway
was involved in DDPF-20-induced cell death and inhibited angiogenesis in vivo.

Conclusion:

This study suggested that the novel PARP inhibitor DDPF-20 may have therapeutic potential in treating
lung cancer.
Nanostructured Lipid Carriers: A Promising Carrier in Targeted Drug
Delivery System
Authors: Tiwari Abhishek , Palaria Binita , Tiwari Varsha , Aslam Ramsha , Kumar Ashok , Sahoo Biswa Mohan , Kumar Manish , Singh Sunil , Kumar Suresh
Published: February 2022

Nanostructured lipid carriers (NLCs) are considered second-generation
lipid-based pharmaceutical formulations in drug delivery systems. It is a more
efficient drug delivery system that includes the development of a nano-particulate
system, and it is superior to the conventional drug delivery system. Among all
available nanoparticles, NLC has gained more attention due to its superior
characteristics, such as being lipophilic, biodegradable, and biocompatible. This
lipid nanoparticulate system is more advantageous over liposomes, microparticles,
emulsions, and solid lipid nanoparticles. NLCs have emerged as a promising carrier
for the delivery of targeted drug substances via oral, parenteral, topical, pulmonary,
transdermal, and ocular routes. These nanocarriers are utilized for the delivery of
both lipophilic as well as hydrophilic drugs. NLCs are composed of a matrix of
physiological lipids, i.e., solid lipid and liquid lipid, emulsifiers, and water. NLCs
provide an opportunity for large-scale production, ease of preparation with
enhanced encapsulation, targeted efficiency, and reduced toxic effects. This review
is focused on the advantages, limitations, methods of preparation, characterization,
and applications of NLCs.
Development and performance analysis of the Gunungkidul cultural potential application based on progressive web apps
Authors: Setialana Pradana , Ardiansyah Muhammad Nurwidya , Suparmanto Nova
Published: February 2022

Gunungkidul has various cultural potentials that make it a tourist destination. To make it easier for tourists to visit and get tourist destination information, several researchers developed a mobile application-based information system. However, mobile applications have several drawbacks as such as the user must install the application and can only be used on specific operating systems. The purpose of this research is to develop and analyze applications regarding the potential of Gunungkidul culture based on progressive web apps and which can be used without having to install applications and can run in all operating systems. The application development method uses Scrum and Ionic Framework as a framework for the application. The performance analysis method was tested on runtime performance (loading, scripting, rendering, painting, system) and memory usage (min JS Heap and max JS Heap) by using Chrome Developer Tools for 30 tests. The results of the development show that there are 7 features in the application, namely (1) Peta; (2) Geoheritage; (3) Daerah; (4) Cagar Budaya; (5) Kuliner; (6) Seni Adat Tradisi; (7) Agenda. Runtime performance and memory usage test results show the average value on aspects (1) Loading: 33.60 ms; (2) Scripting: 1069.20 ms; (3) Rendering: 84.90 ms; (4) Painting: 22.33; (5) System: 429.67 ms; (6) Min JS Heap: 8.05 MB; and (8) Max JS Heap: 19.51 MB.
Semiotics of mathematics problem-solving in Mason’s generalization
Authors: Fitriyah Ihda Mutimmatul , Arrifadah Yuni , Lailiyah Siti
Published: February 2022

Semiotics are signs that include codes, symbols, words, icons, objects, or gestures. This descriptive-qualitative study aimed to describe the semiotics of mathematics problem-solving in Mason’s generalization. Generalization is a finding pattern process in which students will use the different strategies with some semiotics. The subjects were three of 30 eleventh graders from a senior high school in Gresik Regency, Indonesia, that were selected using the purposive sampling technique. Data was collected through documentation, written test, and interview. Data was analyzed by reducing data, presenting data, and concluding. The results of this study showed that students could present the semiotics of gesture, word, and symbols in the process of Mason’s generalization, even though in several stages or indicators the students could not present semiotics. The absence of semiotics in several stages or indicators was not because students could not do such things, but because students passed or skipped these stages. In the perception of generality stage, the semiotics of gesture, word, and symbols could emerge simultaneously. However, for expression of generality, symbolic expression of generality, and manipulation of generality stage, students did not present the three semiotics’ components simultaneously.
Motivation, self-regulated learning, and their effects on learning outcomes of derivative materials during the Covid-19 pandemic
Authors: Fitria Analisa , Najihah Baiti , Paris M. Amin , Atsnan Muh. Fajaruddin , Nareki Maisea Ledua
Published: February 2022

The study aims to identify the effect of learning motivation and self-regulated learning on learning achievement in “Derivative” learning materials in Trigonometry Functions. The study was field research using the ex-post facto design and quantitative approach during the Covid-19 Pandemic. The population was all grade XII students of a state Islamic senior high school in South Kalimantan, Indonesia. The samples of 24 students were selected using the purposive sampling technique. The data were gathered using a questionnaire and test and through documentation and interview. The data were analysed using the descriptive statistics and the inferential statistics consisting of normality test, linearity test, multicollinearity test, heteroscedasticity test, and multiple linear regression. The result shows that there is an effect of motivation and learning independence during the Covid-19 pandemic, which contributed 90.9% in increasing mathematics learning outcomes, and the rest was affected by other variables. This result is based on the results of F-test (Fcount = 104.948 Ftable =3.47) at the significance level of 5% with a multiple linear regression model Ῠ= 13.557+0.435 X1+0.395 X2 + ɛ.
Mathematics pre-service teacher's metacognitive failure in mathematics online learning
Authors: Alifiani Alifiani , Faradiba Surya Sari
Published: February 2022

This study aims to reveal the metacognitive failures experienced by mathematics pre-service teachers based on their mistakes when solving problems in online learning during the pandemic era. This case study involved 29 participants who attended the mathematical problem test and cognitive style test, the two participants were categorized based on their cognitive style: Field Dependence (FD) and Field Independence (FI). The instrument used was a mathematical problem test to collect data on metacognitive that adapted from Stewart and a cognitive styles test to categorize the cognitive style that adapted from the Group Embedded Figures Test (GEFT). An interview was conducted to determine the nature of mathematical error based on metacognitive failure. The description of data analysis and interpretation of the meaning of the findings applied the text analysis. The results showed the different metacognitive failures of the two participants. The metacognitive failure of FI student was categorized as metacognitive blindness and the FD student was categorized as metacognitive stagnation, a new condition of metacognitive failure that was found in this study.
Algorithm-Enabled, Personalized Glucose Management for Type 1 Diabetes at the Population Scale: Prospective Evaluation in Clinical Practice
Authors: Scheinker David , Gu Angela , Grossman Joshua , Ward Andrew , Ayerdi Oseas , Miller Daniel , Leverenz Jeannine , Hood Korey , Lee Ming Yeh , Maahs David M , Prahalad Priya
Published: February 2022

Background

The use of continuous glucose monitors (CGMs) is recommended as the standard of care by the American Diabetes Association for individuals with type 1 diabetes (T1D). Few hardware-agnostic, open-source, whole-population tools are available to facilitate the use of CGM data by clinicians such as physicians and certified diabetes educators.

Objective

This study aimed to develop a tool that identifies patients appropriate for contact using an asynchronous message through electronic medical records while minimizing the number of patients reviewed by a certified diabetes educator or physician using the tool.

Methods

We used consensus guidelines to develop timely interventions for diabetes excellence (TIDE), an open-source hardware-agnostic tool to analyze CGM data to identify patients with deteriorating glucose control by generating generic flags (eg, mean glucose [MG] >170 mg/dL) and personalized flags (eg, MG increased by >10 mg/dL). In a prospective 7-week study in a pediatric T1D clinic, we measured the sensitivity of TIDE in identifying patients appropriate for contact and the number of patients reviewed. We simulated measures of the workload generated by TIDE, including the average number of time in range (TIR) flags per patient per review period, on a convenience sample of eight external data sets, 6 from clinical trials and 2 donated by research foundations.

Results

Over the 7 weeks of evaluation, the clinical population increased from 56 to 64 patients. The mean sensitivity was 99% (242/245; SD 2.5%), and the mean reduction in the number of patients reviewed was 42.6% (182/427; SD 10.9%). The 8 external data sets contained 1365 patients with 30,017 weeks of data collected by 7 types of CGMs. The rates of generic and personalized TIR flags per patient per review period were, respectively, 0.15 and 0.12 in the data set with the lowest average MG (141 mg/dL) and 0.95 and 0.22 in the data set with the highest average MG (207 mg/dL).

Conclusions

TIDE is an open-source hardware-agnostic tool for personalized analysis of CGM data at the clinical population scale. In a pediatric T1D clinic, TIDE identified 99% of patients appropriate for contact using an asynchronous message through electronic medical records while reducing the number of patients reviewed by certified diabetes care and education specialists by 43%. For each of the 8 external data sets, simulation of the use of TIDE produced fewer than 0.25 personalized TIR flags per patient per review period. The use of TIDE to support telemedicine-based T1D care may facilitate sensitive and efficient guideline-based population health management.
The Impact of COVID-19 Lockdown on Daily Activities, Cognitions, and Stress in a Lonely and Distressed Population: Temporal Dynamic Network Analysis
Authors: Haucke Matthias , Heinz Andreas , Liu Shuyan , Heinzel Stephan
Published: February 2022

Background

The COVID-19 pandemic and its associated lockdown measures impacted mental health worldwide. However, the temporal dynamics of causal factors that modulate mental health during lockdown are not well understood.

Objective

We aimed to understand how a COVID-19 lockdown changes the temporal dynamics of loneliness and other factors affecting mental health. This is the first study that compares network characteristics between lockdown stages to prioritize mental health intervention targets.

Methods

We combined ecological momentary assessments with wrist-worn motion tracking to investigate the mechanism and changes in network centrality of symptoms and behaviors before and during lockdown. A total of 258 participants who reported at least mild loneliness and distress were assessed 8 times a day for 7 consecutive days over a 213-day period from August 8, 2020, through March 9, 2021, in Germany, covering a “no-lockdown” and a “lockdown” stage. COVID-19–related worry, information-seeking, perceived restriction, and loneliness were assessed by digital visual analog scales ranging from 0 to 100. Social activity was assessed on a 7-point Likert scale, while physical activity was recorded from wrist-worn actigraphy devices.

Results

We built a multilevel vector autoregressive model to estimate dynamic networks. To compare network characteristics between a no-lockdown stage and a lockdown stage, we performed permutation tests. During lockdown, loneliness had the highest impact within the network, as indicated by its centrality index (ie, an index to identify variables that have a strong influence on the other variables). Moreover, during lockdown, the centrality of loneliness significantly increased. Physical activity contributed to a decrease in loneliness amid the lockdown stage.

Conclusions

The COVID-19 lockdown increased the central role of loneliness in triggering stress-related behaviors and cognition. Our study indicates that loneliness should be prioritized in mental health interventions during lockdown. Moreover, physical activity can serve as a buffer for loneliness amid social restrictions.
A Web-Based Well-being Program for Health Care Workers (Thrive): Protocol for a Randomized Controlled Trial
Authors: Egan Luke A , Mulcahy Mary , Tuqiri Karen , Gatt Justine M
Published: February 2022

Background

Mental health has come to be understood as not merely the absence of mental illness but also the presence of mental well-being, and recent interventions have sought to increase well-being in various populations. A population that deserves particular attention is that of health care workers, whose occupations entail high levels of stress, especially given the ongoing COVID-19 pandemic. A neuroscience-based web-based well-being program for health care workers—the Thrive program—has been newly developed to promote habits and activities that contribute to brain health and overall mental well-being.

Objective

This paper describes the protocol for a randomized controlled trial whose objective is to evaluate the Thrive program in comparison with an active control condition to measure whether the program is effective at increasing well-being and decreasing symptoms of psychological distress in health care workers at a designated Australian hospital.

Methods

The trial will comprise two groups (intervention vs active control) and 4 measurement occasions over a 12-week period. A survey will be administered in each of weeks 0, 4, 8, and 12, and the well-being program will be delivered in weeks 1-7 (via web-based video presentations or digital pamphlets). Each of the 4 surveys will comprise a range of questionnaires to measure well-being, psychological distress, and other key variables. The planned analyses will estimate group-by-time interaction effects to test the hypothesis that mental health will increase over time in the intervention condition relative to the active control condition.

Results

The Thrive program was delivered to a small number of wards at the hospital between February 2021 and July 2021, and it will be delivered to the remaining wards from October 2021 to December 2021. A power calculation has recommended a sample size of at least 200 participants in total. A linear mixed model will be used to estimate the interaction effects.

Conclusions

This trial seeks to evaluate a new web-based well-being program for health care workers at a major public hospital. It will contribute to the growing body of research on mental well-being and ways to promote it.

Trial Registration

Australian New Zealand Clinical Trials Registry ACTRN12621000027819; https://tinyurl.com/58wwjut9

International Registered Report Identifier (IRRID)

DERR1-10.2196/34005
Strengthening Social Capital to Address Isolation and Loneliness in Long-term Care Facilities During the COVID-19 Pandemic: Protocol for a Systematic Review of Research on Information and Communication Technologies
Authors: Beogo Idrissa , Sia Drissa , Tchouaket Nguemeleu Eric , Zhao Junqiang , Gagnon Marie-Pierre , Etowa Josephine
Published: February 2022

Background

The COVID-19 pandemic has had the greatest impact in long-term care facilities (LTCFs) by disproportionately harming older adults and heightening social isolation and loneliness (SIL). Living in close quarters with others and in need of around-the-clock assistance, interactions with older adults, which were previously in person, have been replaced by virtual chatting using information and communication technologies (ICTs). ICT applications such as FaceTime, Zoom, and Microsoft Teams video chatting have been overwhelmingly used by families to maintain residents’ social capital and subsequently reduce their SIL.

Objective

Because of the lack of substantive knowledge on this ever-increasing form of social communication, this systematic review intends to synthesize the effects of ICT interventions to address SIL among residents in LTCFs during the COVID-19 period.

Methods

We will include studies published in Chinese, English, and French from December 2019 onwards. Beyond the traditional search strategy approach, 4 of the 12 electronic databases to be queried will be in Chinese. We will include quantitative and intervention studies as well as qualitative and mixed methods designs. Using a 2-person approach, the principal investigator and one author will blindly screen eligible articles, extract data, and assess risk of bias. In order to improve the first round of screening, a pilot-tested algorithm will be used. Disagreements will be resolved through discussion with a third author. Results will be presented as structured summaries of the included studies. We plan to conduct a meta-analysis if sufficient data are available.

Results

A total of 1803 articles have been retrieved to date. Queries of the Chinese databases are ongoing. The systematic review and subsequent manuscript will be completed by the fall of 2022.

Conclusions

ICT applications have become a promising avenue to reduce SIL by providing a way to maintain communication between LTCF residents and their families and will certainly remain in the post–COVID-19 period. This review will investigate and describe context-pertinent and high-quality programs and initiatives to inform, at the macro level, policy makers and researchers, frontline managers, and families. These methods will remain relevant in the post–COVID-19 era.

International Registered Report Identifier (IRRID)

DERR1-10.2196/36269
Subjektivitas Imperfek: Perempuan dalam "Second Account" di Instagram
Authors: Sirait Amelia Rugun
Published: February 2022

Instagram adalah media sosial populer yang berbasis pada gambar. Penggunaannya memiliki akun yang biasanya untuk profil dirinya sendiri sebagai ruang ekspresi yang dapat disesuaikan dengan kebutuhan. Seiring berkembangnya fitur di Instagram, seseorang dapat memiliki lebih dari satu akun. Ada akun yang dikhususkan untuk lingkaran pertemanan tertentu disebut dengan second account, sebagai akun privat yang digunakan untuk berekspresi dan berelasi melalui gambar. Mereka memiliki kesadaran untuk menghindari relasi dan tatapan dari akun utama mereka, sehingga mereka membangun subjektivitas yang berbeda dalam second account mereka. Ketika berelasi dalam ruang lingkup yang kecil dan terpercaya, tiga orang perempuan subyek riset menunjukkan diri mereka yang ‘sebenarnya’. Apa yang dimaksud dengan subjektivitas yang mereka tunjukkan ini? Unggahan mereka di second account berupa cerita yang dibagikan kepada orang-orang terpercaya. Dalam ruang yang aman ini, terbangunlah subjektivitas yang tidak sempurna, atau subjektivitas imperfek. Melalui riset etnografi media sosial, saya mengikuti cerita dari tiga orang perempuan mengenai akun kedua (second account) mereka.
Membayangkan Tanah Air di Alam: ‘Heimat’ dalam Relasi Manusia-Hutan di Jerman Selatan
Authors: Fahroji Fahmi Rizki
Published: February 2022

Hal apa yang cocok untuk menjelaskan fenomena keterikatan manusia dan hutan di ruang waktu yang kompleks ini, ketika kehidupan tampaknya tidak lagi menyentuh hubungan yang tradisional? Tulisan ini hendak menjelaskan identitas Jerman dalam representasi wacana kehutanan yang tidak hanya muncul sebagai gejala alam, melainkan juga sebagai sumber kehidupan (anima) yang bermuara pada narasi tanah air. Dalam penelitian ini, data empiris mengenai heimat dikumpulkan menggunakan metode penelitian etnografi yang dilakukan di Waldhaus, Freiburg. Tulisan ini juga akan melihat secara ringkas seperti apa sejarah penggunaan paradigma ke-‘tanah-air’-an dari generasi ke generasi hingga penelitian ini berlangsung dalam relasi manusia-hutan di Jerman Selatan. Melalui konsep tradisional heimat, diskursus dan narasi pelibatan manusia-hutan banyak diceritakan sebagai realitas kultural yang mengakar dari masa lampau. Heimat yang dipopulerkan Svasek (2002) dikaitkan pula pada arkeologi pengetahuannya Foucault (2002) menjadi arena penting dalam menafsirkan gejala hubungan multispesies ini dalam kacamata antropologis. Studi ini penting dilakukan karena sejauh corak kebudayaan bisa dilihat pada aspek multidimensional, seperti hubungan hutan dan orang Jerman, maka ideologis kultural Jerman Selatan di hutan akan menjadi suatu penyempurnaan dalam kajian-kajian sebelumnya yang lebih berorientasi literal, politis, sosial namun apolitis, dan historis.
Ketika Pak Pos Tidak Hanya Mengantar Surat: Jasa Pengiriman Pos Indonesia di Mata Pedagang Online
Authors: Viollina Selma Soraya
Published: February 2022

Artikel ini bertujuan untuk mendeskripsikan pandangan pedagang online terhadap jasa pengiriman barang di era e-commerce saat ini khususnya jasa pengiriman milik PT Pos Indonesia yang sebelumnya memiliki fokus kerja dalam pengantaran surat menyurat. Metode etnografi dilakukan untuk pengumpulan data kualitatif melalui observasi serta wawancara mendalam terhadap lima informan yang merupakan pedagang online, di mana kelima informan tersebut dipilih dengan menggunakan teknik purposive sampling. Hasil penelitian menunjukkan bahwa pedagang online memiliki pengalaman yang beragam selama menggunakan jasa pengiriman Pos Indonesia, baik untuk kebutuhan berdagang maupun kebutuhan pengiriman pribadi. Dari pengalaman tersebut, terdapat tiga poin utama yang turut membentuk pandangan pedagang online terhadap jasa pengiriman Pos Indonesia, yaitu 1) Pos Indonesia sebagai BUMN, 2) tarif ongkos kirim yang murah, 3) Pos Indonesia dalam beradaptasi dengan perkembangan zaman.
Menggambar Bintang: Kisah Anak Suku Asmat --Etnografi Sebagai Sarana Edukasi--
Authors: Soselisa Priyanca Minerva Charillane
Published: February 2022

Tulisan ini adalah ulasan sebuah novel etnografi dengan judul ‘Menggambar Bintang: Kisah Anak Suku Asmat’ yang ditulis oleh Dewi Linggasari (2020). Novel etnografi ini adalah salah satu cara Linggasari untuk merekam tradisi dan pola kehidupan masyarakat Asmat, juga perjuangan dalam usaha menggambar bintang – menempuh pendidikan. Tujuan utama penulisan ulasan ini, perlu diingat, bukan untuk mengkritik ataupun mengkaji konflik yang Linggasari anggap kerap atau berulang terjadi di kehidupan suku Asmat, melainkan untuk mengulas dan menganalisis posisi seorang etnografer dalam penulisan etnografi fiksi yang dirasa berpotensi untuk menjangkau pembaca non-etnografer yang lebih luas. Hal ini juga dapat mengacu pada potensi sebuah novel etnografi untuk mengedukasi masyarakat umum untuk menyadari isu yang ada di masyarakat sejak masih usia belia. Dengan tujuan tersebut maka ulasan ini akan dimulai dengan penjelasan mengenai definisi dan tujuan umum etnografi juga etnografi fiksi, setelah itu akan dilanjutkan dengan pembahasan isi dari novel etnografi oleh Linggasari, akhirnya akan ditutup dengan analisa akan novel tersebut.
Aktivisme Melampaui Laga: Perlawanan dan Gerakan Sosial Ultras di Freiburg
Authors: Pratama Aditya R.
Published: February 2022

Corrillo Ultras dan Supporter Crew SC Freiburg merupakan komunitas suporter sepakbola bergaya Ultras. Di Jerman sendiri, Ultras merupakan format yang populer. Beberapa stereotip dan stigma buruk masih melekat pada kelompok Ultras. Mulai dari suporter yang lekat dengan kekerasan dan vandalisme, hingga keterkaitannya dengan paham sayap kanan dan Neo-Nazi. Bertentangan dengan stereotip yang ada, Corrillo Ultras dan Supporter Crew SC Freiburg malah melakukan berbagai kegiatan sosial yang fokus kepada kritik terhadap perilaku diskriminatif dan rasisme. Muncul beberapa pertanyaan: mengapa Corrillo Ultras dan Suporter Crew SC Freiburg aktif terlibat dalam program sosial? Bagaimana dua kelompok Ultras di Freiburg memersepsikan isu sosial yang sedang berkembang?
Utilization of Waste Plastic in Manufacturing of Paver Blocks
Authors: soni Aaryakant , Rajput Toshan Singh , Sahu Kuldeep , Rajak Sachin
Published: February 2022

Abstract: The aim of this project is to replace cement with plastic waste in paver block and to reduce the cost of paver block when compared to that of convention concrete paver blocks. At present nearly 56 lakhs tones of plastic waste is produced in India per year. The degradation rate of plastic waste is also a very slow process. Hence the project is helpful in reducing plastic waste in a useful way. In this project we have used plastic waste in different proportions with quarry dust, coarse aggregate and ceramic waste. The paver blocks were prepared and tested and the results were discussed. Keywords: Paver block, Plastic waste, Ceramic waste
Immunodetection of Adhesin Pili Protein 38.6 kDa K. pneumoniae Using Western Blot
Authors: Agustina Dini , Mufida Diana Chusna , Saffanah Nurul Indah
Published: February 2022

Abstract: Klebsiella pneumoniae is a pathogen that causes infections in communities and hospitals. These bacteria have many virulence factors that play an important role in the pathogenicity of infection and antibiotic resistance. Pili protein Klebsiella pneumoniae with 38.6 kDa as one of the virulence factors, that have roles as hemagglutinin dan adhesin protein so potentially as a vaccine candidate. This research aimed to immunodetection pili protein 38.6 kDa using Western Blot.  Western Blot was used to detect that protein with specific primary antibodies. These antibodies are obtained from mice serum-induced intraperitoneally with Pili protein 38.6 kDa Klebsiella pneumoniae. Protein bands that appear on the membrane of Western Blot results are proteins with a molecular weight of 85.6 kDa, 65.5 kDa, 46.9 kDa, and 29.4 kDa. This study concludes that Pili protein 38.6 kDa Klebsiella pneumoniae as the target protein does not appear in the Western Blot result.Abstrak: Klebsiella pneumoniae adalah patogen yang menyebabkan infeksi di komunitas maupun rumah sakit. Bakteri tersebut memiliki banyak faktor virulensi yang berperan penting dalam patogenisitas terjadinya infeksi dan resistensi antibiotik. Protein pili Klebsiella pneumoniae dengan 38,6 kDa sebagai salah satu faktor virulensi, memiliki peran sebagai protein hemagglutinin dan adhesin sehingga berpotensi sebagai kandidat vaksin. Tujuan penelitian ini untuk imunodeteksi protein pili 38,6 kDa dengan menggunakan Western Blot. Western Blot digunakan untuk mendeteksi protein itu dengan spesifik antibodi primer. Antibodi ini diperoleh dari serum tikus yang telah diinduksi secara inteperitoneal dengan protein Pili 38,6 kDa Klebsiella pneumoniae. Band protein yang muncul pada membran hasil Western Blot adalah protein dengan berat molekul 85,6 kDa; 65,5 kDa; 46,9 kDa dan 29,4 kDa. Dari penelitian ini dapat disimpulkan bahwa protein Pili 38.6 kDa Klebsiella pneumoniae sebagai protein target tidak muncul.
The Type Fragmentation Patterns Confirmed Acetaminophen By Using Liquid Chromatography-Mass Spectroscopy (LCMS) From Herbal Medicine (Jamu)
Authors: Taupik Muhammad , Djuwarno Endah Nurrohwinta , Mustapa Moh Adam , Kunusa Wiwin R , La Kilo Jafar , Sahumena Muhamad Handoyo
Published: February 2022

Abstract: The Jamu or herbal medicine were adulterated with chemical drugs in the last twenty years. One of the chemical drugs that are commonly added to herbal medicine is acetaminophen. Acetaminophen is generally considered an analgesic-antipyretic drug. The research aims to identify the content of paracetamol and its amount added to the jamu. This study consisted of 6 jamu or herbal medicine samples with different brands. The TLC method conducted the qualitative analysis using the mobile phase n-hexane: ethyl acetate (1:1). Three positive samples containing acetaminophen were obtained: D, E, and F. The three positive samples containing acetaminophen were calculated using the LCMS method. Quantitative analysis was conducted with LCMS using a reversed-phase with a mobile phase of acetonitrile: water (15%>: 85%> v/v) at a flow rate of 0.2 mL/min and an injection volume of 5 µL, it was obtained the levels of acetaminophen in sample D as much as  0.16 g/2 g of sample, in sample E of 0.63 g/7 g of sample, and sample F of 0.56 g/7 g of sample. Although the acetaminophen content in herbal medicine is relatively small, according to the Regulation of the Minister of Health of Indonesia No. 007 of 2012, traditional medicine or herbal medicine should not contain synthetic chemicals.Abstrak: Dalam dua puluh tahun terakhir, banyak jamu yang dipalsukan dengan obat-obatan kimia. Salah satu obat kimia yang biasa ditambahkan pada jamu adalah asetaminofen. Acetaminophen umumnya dianggap sebagai obat analgesik-antipiretik. Penelitian ini bertujuan untuk mengetahui kandungan parasetamol dan jumlah yang ditambahkan pada jamu. Sampel penelitian ini terdiri dari 6 sampel jamu dengan merek yang berbeda. Analisis kualitatif dilakukan dengan metode KLT menggunakan fase gerak n-heksana: etil asetat (1:1). Diperoleh tiga sampel positif mengandung asetaminofen, yaitu sampel D, E, dan F. Tiga sampel positif mengandung asetaminofen dihitung menggunakan metode LCMS. Analisis kuantitatif dilakukan dengan LCMS menggunakan fase terbalik dengan fase gerak asetonitril: air (15%>:85%> v/v) pada laju alir 0,2 mL/menit dan volume injeksi 5 µL. Diperoleh kadar asetaminofen pada sampel D sebanyak 0,16 g/2 g sampel, pada sampel E sebesar 0,63 g/7 g sampel, dan pada sampel F sebesar 0,56 g/7 g sampel. Meskipun kandungan asetaminofen dalam jamu relatif sedikit, namun menurut Peraturan Menteri Kesehatan RI No. 007 Tahun 2012, obat tradisional atau jamu tidak boleh mengandung bahan kimia sintetik.
A Pyrolysis of 80 Mesh Spirulina platensis Residue (SPR) With Fe3O4 Catalyst
Authors: Jamilatun Siti , Shitophyta Lukhi Mulia , Nisa Heidy Oktavia , Nurhidayah Mutia Endar
Published: February 2022

Abstract: Spirulina platensis Residue (SPR) is obtained from the solid residue of Spirulina platensis extraction, which has high carbohydrate and protein content. Pyrolysis technology will convert SPR into a bio-oil, water phase, char, and gas (CO, CO2, H2, CH4) product with the potential as an environmentally friendly and sustainable fuel. This study aims to obtain data on the optimum conditions and product yield composition of SPR catalytic pyrolysis using the in-situ method.  Pyrolysis occurs in a fixed-bed reactor with SPR 50 g feed and Fe3O4 catalyst (2.5 g and 5 g) at temperature variations of 300-600 °C with an average heating rate of 14.07 °C/min. The higher the pyrolysis temperature, the more bio-oil yield, the optimum at a temperature of 500 ⁰C. The use of 2.5 and 5-gram catalysts obtained bio-oil yields of 25.01 and 17.67 %. The yield of biochar will be lower with increasing pyrolysis temperature. The optimum condition for the gas production was achieved at 300 °C, using a catalyst of 2.5 and 5 grams, the yield of gas was 23.59, and 19.74 %. Moreover, the smaller the specific gravity and the higher the bio-oils pH, the better it is as a vehicle fuel.Abstrak: Spirulina platensis Residu (SPR) diperoleh dari residu padat ekstraksi Spirulina platensis yang memiliki kandungan karbohidrat dan protein tinggi. Teknologi pirolisis akan mengubah SPR menjadi produk bio-oil yang berpotensi sebagai bahan bakar ramah lingkungan dan berkelanjutan. Penelitian ini bertujuan untuk mendapatkan data kondisi optimum dan komposisi yield produk dari pirolisis katalitik SPR dengan metode in-situ. Pirolisis SPR akan menghasilkan produk berupa bio-oil, water phase, char dan gas (CO, CO2, H2, CH4). Pirolisis berlangsung dalam reaktor fixed-bed dengan umpan SPR 50 g serta katalis Fe3O4 (2.5 g dan  5 g)  pada variasi suhu 300-600°C dengan heating rate rata-rata 14,07 °C/min. Semakin tinggi suhu pirolisis semakin banyak yield bio-oil, optimum pada suhu 500 ⁰C. Pemakaian katalis 2,5 dan 5 gram diperoleh yield bio-oil sebanyak 25,01 dan 17,67 %. Yield biochar akan semakin rendah dengan kenaikan suhu pirolisis. Kondisi optimum produk gas dicapai pada 300 °C dengan katalis 2,5 dan 5 gram diperoleh yield gas sebesar 23,59, dan 19,74 %. Hal tersebut mengakibatkan, semakin kecil berat jenis dan semakin tinggi pH bio-oil maka semakin baik sebagai bahan bakar kendaraan.
Climate-Responsiveness Embodied in Modern Mosque Design
Authors: Rauzi Era Nopera , Hasan Izziah , Sari Laina Hilma
Published: February 2022

Abstract: A mosque is a place of worship for Muslims. Modernity concept to visualize the simple form in a mosque is relevant, which would help the worshippers to be more solemn. Office buildings, mainly located in Aceh, known as Serambi Mekkah, are required to provide a mosque in the office environment. Since its concept transforms from traditional style to modern style, the mosque in the office area is designed in modernity. The study purposes of enabling climate-responsive principles in mosque design for achieving indoor thermal comfort. Even though without available power support. The design method approached problem-solving by creating the building friendly to the environment and achieving indoor thermal comfort. This study used trial and error through modeling appropriate to climate-responsiveness mosque design in the office area. Furthermore, this study would be continued in complex buildings, namely schools and offices, promoting environmentally-friendly to be applied in building design. Abstrak: Masjid adalah tempat ibadah bagi umat Islam. Konsep modernitas untuk memvisualisasikan bentuk sederhana di masjid adalah relevan, yang akan membantu jamaah untuk lebih khusyuk. Gedung perkantoran khususnya yang berada di Aceh yang dikenal dengan Serambi Mekkah wajib menyediakan mushola di lingkungan kantor. Karena konsepnya bertransformasi dari gaya tradisional ke gaya modern, masjid di area perkantoran juga didesain dengan gaya modern. Tujuan studi ini adalah untuk mengaktifkan prinsip responsif iklim dalam desain masjid untuk mencapai kenyamanan termal dalam ruangan meskipun tanpa dukungan daya yang tersedia. Metode desain pada studi ini menggunakanpendekatan pemecahan masalah untuk menciptakan bangunan ramah lingkungan dan mencapai kenyamanan termal dalam ruangan. Penelitian ini menggunakan metode trial and error melalui pemodelan yang sesuai dengan desain masjid yang responsif terhadap iklim di kawasan perkantoran. Selanjutnya studi ini akan dilanjutkan penerapannya pada bangunan kompleks seperti sekolah dan perkantoran untuk mempromosikan desain bangunan ramah lingkungan.
The effectiveness of jigsaw learning strategy to teach speaking
Authors: Widodo Urip , Mugiyo Mugiyo
Published: February 2022

This research aims to know whether the jigsaw learning strategy is better than the audio-lingual method to teach speaking. This experimental research was carried out in the vocational school. The population was four classes (140 students) of eleventh grade A, B, C, D, and E. The samples were the eleventh grade A as the experimental group and B as the control group. Each group consists of 30 students. The experimental group was taught using the Jigsaw learning strategy, while the control group used the Audio-Lingual Method. The data collection technique was through the speaking test, and the data analysis technique was through descriptive statistics and inferential statistics. The data analysis shows that the mean score of the students who were taught by using the jigsaw learning strategy is 81,23, while the mean score of the students who were taught by using the audio-lingual method is 76,03. Moreover, the Fo (8,77) is higher than Ft (4,00) at the significance level of significance α= 0,05, which means the null hypotheses are rejected, and the alternative hypotheses are accepted. In conclusion, the jigsaw learning strategy is more effective than the audio-lingual method to teach speaking.
Problem-based learning on students' English learning interests
Authors: Qomariyah Siti Syafi’atul , Utama I Made Permadi
Published: February 2022

This study was conducted to find out how Problem Based Learning (PBL) affects students' English learning interests. The questionnaire is used as an instrument. The result of implementing the Problem Based Learning (PBL) method was that students felt enjoy and more interested. The students could develop their brainstorming when the teacher provided a problem to discuss with their group. They also created some ideas for solving their problem. Some students also provide their daily problems and make the classroom/discussion situation life. Based on data analysis showed that computing of the t-test was significant 0.000. It means that applying Problem Based learning (PBLaffects students' learning interests. So, this method can be used as one of the alternative ways of teaching-learning to develop students' learning interests.
Review of The Literature, Prospects, and The Impact of Bancassurance on The Stability of The Banking Industry
Authors: Nasution Anita Hakim , Lukmandono Lukmandono
Published: February 2022

Existing literature, as well as empirical studies on Bancassurance, emphasize that there has been a shifting business in the bank from selling bank products to IKNB (Non-Bank Financial Industry). In this study, Bancassurance is one of the IKNBs that must be sold because the feather-based income is quite large and has a small risk because the bank is a referral as a distribution agent. The purpose of this research is to determine the characteristics of Bancassurance in various countries for the first time in Indonesia so that our human resources are not unfamiliar with the insurance sold in banks and can adjust the strategy to market characteristics in Indonesia. This paper discusses Bancassurance with various methodologies ranging from Expert Judgment to Risk Management by involving 15 international journal studies related to the implementation of Bancassurance in various countries including Google Scholar, Elsevier, and Science Direct. This paper will be useful as a topic for further research where researchers can identify the effect of bancassurance on banking, risks that occur in bancassurance practice, solutions to sales strategies, and system bottlenecks that occur between bank sales and sales bancassurance. The result of this research is the reluctance of selling banks to sell insurance products because they do not understand the concept of life insurance and unit link. In addition, bancassurance sales resources placed in the bank are not as good as insurance agents in general due to different incentives, incentive problems, and bottlenecks in the sales execution flow also make insurance targets cannot be achieved optimally. The solution is that Re-skills must be done in the "Virtual Century" era because the insurance link unit has also shifted from offline meetings to online so that all systems are done digitally. Coordination between resources is expected to go through a digitization process so that customer claims are quickly resolved, referrals are quickly executed and periodic coaching can be carried out by prohibited branch heads/superiors. Bancassurance with unit link customization/personalization will be a new value proposition.
HIGH SCHOOL STUDENTS’ ERROR IN SOLVING WORD PROBLEM OF TRIGONOMETRY BASED ON NEWMAN ERROR HIERARCHICAL MODEL
Authors: Wardhani Thalitha Ariesti Widhia , Argaswari Deshinta P. A. D.
Published: February 2022

This study aims to identify students’ errors in solving word problems of trigonometry according to the Newman Error Hierarchical Model. This study uses qualitative descriptive data gathered based on fact, and data analysis presented descriptively. The population is students of X IPA 2 at SMAN 1 Cikarang Utara, West Java, Indonesia, and to understand more about the error that happened, the researcher took six students of SMAN 1 Cikarang Utara as a sample. The sample is chosen by purposive sampling. Instrument tests, interviews, and documentation are the data collected. This study used the Newman Error Hierarchical Model to analyze high school students’ errors in solving word problems of trigonometry. The results show that students made errors in comprehension, transformation, process skill, and encoding stage. The errors were caused by their lack of understanding some words and terms in the word problem, lack of ability in transforming the word problem to the mathematical model and strategy, lack of processing the algebra and calculation, lack of motivation, and carelessness.
Macroeconomic factors and emerging equity market: A contextual analysis using quantile regression
Authors: Bhattacharya Mousumi , Bhattacharya Sharad Nath
Published: February 2022

This article examines the role of macroeconomic factors in influencing Indian stock market movements across different market conditions. The study is important for market participants and policymakers as macroeconomic factors may be the source of systematic risk that influences the stock market. We employ factor analysis as a solution to the multicollinearity issues associated with multiple macroeconomic factors. Using three statistical factors built from macroeconomic factors, we show how they impact the stock market, particularly during up and down market conditions. While the influence of foreign exchange rate, broad money supply, economic growth, wholesale inflation, global equity markets, and export is positive and stable across market conditions, an inverse relationship between contemporaneous bond yield and equity market movements is evidenced. Gold and foreign institutional investment inflows seem to exert an increasingly negative influence on market movements at extreme up-market conditions. These findings call for active intervention by policymakers to stabilise the market during extreme market conditions
Neue-Gemeinschaft: Digital Transformation of Micro, Small and Medium Enterprises among Rural Community in Banyuwangi, East Java
Authors: Mazya Thita , M Kolopaking Lala , Hadi Dharmawan Arya , Ridho Nurrochmat Dodik , Satria Arif
Published: February 2022

This study aims to analyze the digital transformation of changing rural economy towards a digital society. Qualitative research methods were used, including 50 participants and in-depth interview informants, with participatory observation, policy analysis, and three series of focus group discussions both in person and online. The study found that internet intervention significantly impacts collective behaviour and improves MSMEs development. Referring to the continuum theory of Gemeinschaft-Gesellschaft, the study showed that these changes result in the formation of a ‘Neue Gemeinschaft’. Rural MSMEs are entering a new era due to the adoption of digital technology by businesses that support and exhibit characteristics such as the intensity of the use of internet networks, the ability to conduct digital transactions, and the preservation of village social values. Theoretically and empirically, the research indicates that digital transformation is driven by a change in collective behaviour around technology use, followed by inclusive rural digital economy.
Surgical excision of complex lipoma from the foot: a case report
Authors: Uddin Akram , Flanagan George , Reilly Ian
Published: February 2022

Soft tissue malignant tumours of the foot and ankle are rare. Diagnostic
imaging and interventional biopsy are vital to establish the nature and
grading of a suspicious tumour prior to surgical intervention.
Combined effects of double nonlocal terms in the nonlinear eigenvalue problems
Authors: Yao Shuai , Chen Haibo
Published: February 2022

In this paper, we study the following eigenvalue problem for Kirchhoff
type equation with Hartree nonlinearity:
\begin{equation}
-M\left(\int_{\mathbb{R}^{N}}|\nabla
u|^{2}dx\right)\Delta
u+\mu V(x)u=\left(
I_{\alpha }\ast
Q\left\vert
u\right\vert^{p}\right)
Q\vert u\vert
^{p-2}u+\lambda f(x)u \quad
\text{in}\quad
\mathbb{R}^{N}, \end{equation}
where $N\geq3, a, \mu>0$
parameters, $M(t)=at+1$, $V\in
C(\mathbb{R}^{N},\mathbb{R}%
^{+}) $, $I_{\alpha }$ is the Riesz potential,
$Q(x)\in
L^{\infty}(\mathbb{R}^{N})$
with changes sign in
$\overline{\Omega}:=\left\{V(x)=0\right\}$,
and $0
Pneumonia During COVID-19 Pandemic in Hospitalized Children. Characterization of SARS-COV2 Pneumonia . Multi-Center Cohort Study
Authors: Al-Asy Hassan , Toema Osama , Elmezawy Rehab , Hamza Mohammed , Hasanin Omar , Mabrouk Maali , Aldiba Manal
Published: February 2022

Abstract : Although COVID-19 is milder in young than adults. However, it
can cause pneumonia in children eventually requiring hospitalization.
Clinical similarity between COVID-19, other viral, and bacterial
pneumonia at initial presentation of the disease caused a great
challenge. We aimed to differentiate COVID-19 pneumonia from other viral
and bacterial pneumonia in children, and to characterize it . Materials
and Methods: This study included 300 children, who were hospitalized
with clinically and radiologically confirmed pneumonia during COVID-19
pandemic. Clinical symptoms were collected and analyzed . Cultures ,
real time polymerase chain transcriptase test for some respiratory
viruses and SARS-COV2, C-reactive protein, serum procalcitonin ,serum
ferritin , complete blood counts , and ferritin/procalcitonin ratio were
done for all patients Results: This study showed that COVID-19 pneumonia
was only 15 % of all admitted pneumonia cases. It had low proportion of
high fever , mild course, significant lymphopenia , significant
thrombocytopenia, low procalcitonin , low C-reactive protein , higher
ferritin / procalcitonin ratio , and higher neutrophil/ lymphocyte ratio
, significant high percentage of ground glass , and less percentage of
consolidation in CT images . Conclusion: During ( COVID-19) outbreak,
pneumonia due to COVID-19 was less common , and less severe than that
caused by other viruses or bacteria in children. Naso- pharyngeal swabs
for multi-respiratory pathogen, including SARS-COV-2, are required to
not to attribute most pneumonia cases to be due to COVID-19. However ,
further large-sample studies are needed to have full blown picture about
COVID-19 pneumonia in children.