bluuebunny/crossref_metadata_2025 huggingface dataset:
Complemented Palindromic Small RNAs First Discovered from SARS Coronavirus
Authors: Liu Chang , Chen Ze , Hu Yue , Ji Haishuo , Yu Deshui , Shen Wenyuan , Li Siyu , Ruan Jishou , Bu Wenjun , Gao Shan
Published: September 2018

In this study, we report for the first time the existence of complemented palindromic small RNAs (cpsRNAs) and propose that cpsRNAs and palindromic small RNAs (psRNAs) constitute a novel class of small RNAs. The first discovered 19-nt cpsRNA UUAACAAGCUUGUUAAAGA, named SARS-CoV-cpsR-19, was detected from a 22-bp DNA complemented palindrome TCTTTAACAAGCTTGTTAAAGA in the severe acute respiratory syndrome coronavirus (SARS-CoV) genome. The phylogenetic analysis supported that this DNA complemented palindrome originated from bat betacoronavirus. The results of RNA interference (RNAi) experiments showed that one 19-nt segment corresponding to SARS-CoV-cpsR-19 significantly induced cell apoptosis. Using this joint analysis of the molecular function and phylogeny, our results suggested that SARS-CoV-cpsR-19 could play a role in SARS-CoV infection or pathogenesis. The discovery of cpsRNAs has paved a way to find novel markers for pathogen detection and to reveal the mechanisms underlying infection or pathogenesis from a different point of view. Researchers can use cpsRNAs to study the infection or pathogenesis of pathogenic viruses when these viruses are not available. The discovery of psRNAs and cpsRNAs, as a novel class of small RNAs, also inspire researchers to investigate DNA palindromes and DNA complemented palindromes with lengths of psRNAs and cpsRNAs in viral genomes.
Metagenomic Composition Analysis of an Ancient Sequenced Polar Bear Jawbone from Svalbard
Authors: Pratas Diogo , Hosseini Morteza , Grilo Gonçalo , Pinho Armando J. , Silva Raquel M. , Caetano Tânia , Carneiro João , Pereira Filipe
Published: September 2018

The sequencing of ancient DNA samples provides a novel way to find, characterize, and distinguish exogenous genomes of endogenous targets. After sequencing, computational composition analysis enables filtering of undesired sources in the focal organism, with the purpose of improving the quality of assemblies and subsequent data analysis. More importantly, such analysis allows extinct and extant species to be identified without requiring a specific or new sequencing run. However, the identification of exogenous organisms is a complex task, given the nature and degradation of the samples, and the evident necessity of using efficient computational tools, which rely on algorithms that are both fast and highly sensitive. In this work, we relied on a fast and highly sensitive tool, FALCON-meta, which measures similarity against whole-genome reference databases, to analyse the metagenomic composition of an ancient polar bear (Ursus maritimus) jawbone fossil. The fossil was collected in Svalbard, Norway, and has an estimated age of 110,000 to 130,000 years. The FASTQ samples contained 349 GB of nonamplified shotgun sequencing data. We identified and localized, relative to the FASTQ samples, the genomes with significant similarities to reference microbial genomes, including those of viruses, bacteria, and archaea, and to fungal, mitochondrial, and plastidial sequences. Among other striking features, we found significant similarities between modern-human, some bacterial and viral sequences (contamination) and the organelle sequences of wild carrot and tomato relative to the whole samples. For each exogenous candidate, we ran a damage pattern analysis, which in addition to revealing shallow levels of damage in the plant candidates, identified the source as contamination.
Bioinformatics Analysis of SNPs in IL-6 Gene Promoter of Jinghai Yellow Chickens
Authors: Xin Shijie , Wang Xiaohui , Dai Guojun , Zhang Jingjing , An Tingting , Zou Wenbin , Zhang Genxi , Xie Kaizhou , Wang Jinyu
Published: September 2018

The proinflammatory cytokine, interleukin-6 (IL-6), plays a critical role in many chronic inflammatory diseases, particularly inflammatory bowel disease. To investigate the regulation of IL-6 gene expression at the molecular level, genomic DNA sequencing of Jinghai yellow chickens (Gallus gallus) was performed to detect single-nucleotide polymorphisms (SNPs) in the region −2200 base pairs (bp) upstream to 500 bp downstream of IL-6. Transcription factor binding sites and CpG islands in the IL-6 promoter region were predicted using bioinformatics software. Twenty-eight SNP sites were identified in IL-6. Four of these 28 SNPs, three [−357 (G > A), −447 (C > G), and −663 (A > G)] in the 5′ regulatory region and one in the 3′ non-coding region [3177 (C > T)] are not labelled in GenBank. Bioinformatics analysis revealed 11 SNPs within the promoter region that altered putative transcription factor binding sites. Furthermore, the C-939G mutation in the promoter region may change the number of CpG islands, and SNPs in the 5′ regulatory region may influence IL-6 gene expression by altering transcription factor binding or CpG methylation status. Genetic diversity analysis revealed that the newly discovered A-663G site significantly deviated from Hardy-Weinberg equilibrium. These results provide a basis for further exploration of the promoter function of the IL-6 gene and the relationships of these SNPs to intestinal inflammation resistance in chickens.
Methodology for Improving the Analysis, Interpretation, and Geo-Visualisation of Erosion Rates in Coastal Beaches—Andalusia, Southern Spain
Authors: Prieto-Campos Antonio , Díaz-Cuevas Pilar , Fernandez-Nunez Miriam , Ojeda-Zújar José
Published: September 2018

Erosion is one of the major issues currently facing coastal areas. Some consequences of this process are beach loss and higher flood risk, which will likely be exacerbated given ongoing sea-level rise. With this in mind, those responsible for conservation and management decisions need appropriate tools with which to identify critical coastal areas, as well as to analyse, interpret, and visualise them with the appropriate geomorphological and environmental background. The aim of this work was to present a methodology for improving the analysis and interpretation of coastal erosion rates, as well as to guarantee wide access and dissemination of erosion data. To that end, an approach for the production, management, and dissemination of shoreline erosion data for the Andalusian coast in Southern Spain was developed. This approach enables the analysis and interpretation of the erosion rates in coasts by linking erosion rates with geomorphological and thematic information using a data model. Additionally, this methodology was proven to be a valid and appropriate tool for the design of a web-based viewer, being the best way to represent the erosion rates obtained every 50 m of shore for the entire Andalusian coast, being an exposed coastal front 917 km long. This is particularly useful for integrated coastal zone management schemes, enabling quick and easy access to valuable information.
How Do Investment Banks Price Initial Public Offerings? An Empirical Analysis of Emerging Market
Authors: Rasheed Abdul , Khalid Sohail Muhammad , Din Shahab-Ud , Ijaz Muhammad
Published: September 2018

This study investigates that how investment banks select alternative valuation models to price Initial Public Offerings (IPOs) and examine the value-relevance of each valuation model using the data of 88 IPOs listed on the Pakistan Stock Exchange (PSX) during 2000–2016. This study investigates that investment banks used Dividend Discount Model (DDM), Discounted Cash Flow (DCF) and comparable multiples valuation models on the basis of firm-specific characteristics, aggregate stock market returns and volatility before the IPOs. In this study, a binary logit regression model is used to estimate the cross-sectional determinants of the choice of valuation models by investment banks. The results reveal that underwriters are more likely to use DDM to value firms that have dividends payout trail. The investment banks select DCF when valuing the younger firms, that have more assets-in-tangible, firms that have negative sales growth and positive market returns before the IPO; while comparable multiples are used for mature firms and firms that have less assets-in-tangible. Furthermore, this study also used OLS regressions to examine the value-relevance of each valuation model and Wald-test to examine the predictive power of cross-sectional variation in the market values. The findings unveil that P/B ratio has highest but DCF has lowest predictive power to market values. The Wald-test results depict that none of the valuation methods produces an unbiased estimate of market values.
Actin-Myosin Interaction: Structure, Function and Drug Discovery
Authors: Guhathakurta Piyali , Prochniewicz Ewa , Thomas David D.
Published: September 2018

Actin-myosin interactions play crucial roles in the generation of cellular force and movement. The molecular mechanism involves structural transitions at the interface between actin and myosin’s catalytic domain, and within myosin’s light chain domain, which contains binding sites for essential (ELC) and regulatory light chains (RLC). High-resolution crystal structures of isolated actin and myosin, along with cryo-electron micrographs of actin-myosin complexes, have been used to construct detailed structural models for actin-myosin interactions. However, these methods are limited by disorder, particularly within the light chain domain, and they do not capture the dynamics within this complex under physiological conditions in solution. Here we highlight the contributions of site-directed fluorescent probes and time-resolved fluorescence resonance energy transfer (TR-FRET) in understanding the structural dynamics of the actin-myosin complex in solution. A donor fluorescent probe on actin and an acceptor fluorescent probe on myosin, together with high performance TR-FRET, directly resolves structural states in the bound actin-myosin complex during its interaction with adenosine triphosphate (ATP). Results from these studies have profound implications for understanding the contractile function of actomyosin and establish the feasibility for the discovery of allosteric modulators of the actin-myosin interaction, with the ultimate goal of developing therapies for muscle disorders.
Transcriptome Analysis of Novosphingobium pentaromativorans US6-1 Reveals the Rsh Regulon and Potential Molecular Mechanisms of N-acyl-l-homoserine Lactone Accumulation
Authors: Lu Hang , Huang Yili
Published: September 2018

In most bacteria, a bifunctional Rsh responsible for (p)ppGpp metabolism is the key player in stringent response. To date, no transcriptome-wide study has been conducted to investigate the Rsh regulon, and the molecular mechanism of how Rsh affects the accumulation of N-acyl-l-homoserine lactone (AHL) remains unknown in sphingomonads. In this study, we identified an rshUS6–1 gene by sequence analysis in Novosphingobium pentaromativorans US6-1, a member of the sphingomonads. RNA-seq was used to determine transcription profiles of the wild type and the ppGpp-deficient rshUS6–1 deletion mutant (∆rsh). There were 1540 genes in the RshUS6–1 regulon, including those involved in common traits of sphingomonads such as exopolysaccharide biosynthesis. Furthermore, both RNA-seq and quantitative real-time polymerase chain reaction (qRT-PCR) showed essential genes for AHL production (novI and novR) were positively regulated by RshUS6–1 during the exponential growth phase. A degradation experiment indicated the reason for the AHL absence in ∆rsh was unrelated to the AHL degradation. According to RNA-seq, we proposed σE, DksA, Lon protease and RNA degradation enzymes might be involved in the RshUS6–1-dependent expression of novI and novR. Here, we report the first transcriptome-wide analysis of the Rsh regulon in sphingomonads and investigate the potential mechanisms regulating AHL accumulation, which is an important step towards understanding the regulatory system of stringent response in sphingomonads.
Genome-Wide Identification, Classification and Expression Analysis of the HSP Gene Superfamily in Tea Plant (Camellia sinensis)
Authors: Chen Jiangfei , Gao Tong , Wan Siqing , Zhang Yongheng , Yang Jiankun , Yu Youben , Wang Weidong
Published: September 2018

Heat shock proteins (HSPs) function as molecular chaperones. These proteins are encoded by a multigene family whose members play crucial roles in plant growth, development and stress response. However, little is known about the HSP gene superfamily in tea plant. In this study, a total of 47 CsHSP genes were identified, including 7 CsHSP90, 18 CsHSP70, and 22 CssHSP genes. Phylogenetic and composition analyses showed that CsHSP proteins in the same subfamily have similar gene structures and conserved motifs, but significant differences exist in the different subfamilies. In addition, expression analysis revealed that almost all CsHSP genes were specifically expressed in one or more tissues, and significantly induced under heat and drought stress, implying that CsHSP genes play important roles in tea plant growth, development, and response to heat and drought stress. Furthermore, a potential interaction network dominated by CsHSPs, including HSP70/HSP90 organizing protein (HOP) and heat shock transcription factor (HSF), is closely related to the abovementioned processes. These results increase our understanding of CsHSP genes and their roles in tea plant, and thus, this study could contribute to the cloning and functional analysis of CsHSP genes and their encoded proteins in the future.
Improved Chondrogenic Differentiation of rAAV SOX9-Modified Human MSCs Seeded in Fibrin-Polyurethane Scaffolds in a Hydrodynamic Environment
Authors: Venkatesan Jagadeesh K. , Gardner Oliver , Rey-Rico Ana , Eglin David , Alini Mauro , Stoddart Martin J. , Cucchiarini Magali , Madry Henning
Published: September 2018

The repair of focal articular cartilage defects remains a problem. Combining gene therapy with tissue engineering approaches using bone marrow-derived mesenchymal stem cells (MSCs) may allow the development of improved options for cartilage repair. Here, we examined whether a three-dimensional fibrin-polyurethane scaffold provides a favorable environment for the effective chondrogenic differentiation of human MSCs (hMSCs) overexpressing the cartilage-specific SOX9 transcription factor via recombinant adeno-associated virus (rAAV) -mediated gene transfer cultured in a hydrodynamic environment in vitro. Sustained SOX9 expression was noted in the constructs for at least 21 days, the longest time point evaluated. Such spatially defined SOX9 overexpression enhanced proliferative, metabolic, and chondrogenic activities compared with control (reporter lacZ gene transfer) treatment. Of further note, administration of the SOX9 vector was also capable of delaying premature hypertrophic and osteogenic differentiation in the constructs. This enhancement of chondrogenesis by spatially defined overexpression of human SOX9 demonstrate the potential benefits of using rAAV-modified hMSCs seeded in fibrin-polyurethane scaffolds as a promising approach for implantation in focal cartilage lesions to improve cartilage repair.
RBM4a-SRSF3-MAP4K4 Splicing Cascade Constitutes a Molecular Mechanism for Regulating Brown Adipogenesis
Authors: Peng Hui-Yu , Liang Yu-Chih , Tan Tse-Hua , Chuang Huai-Chia , Lin Ying-Ju , Lin Jung-Chun
Published: September 2018

An increase in mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) reportedly attenuates insulin-mediated signaling which participates in the development of brown adipose tissues (BATs). Nevertheless, the effect of MAP4K4 on brown adipogenesis remains largely uncharacterized. In this study, results of a transcriptome analysis (also referred as RNA-sequencing) showed differential expressions of MAP4K4 or SRSF3 transcripts isolated from distinct stages of embryonic BATs. The discriminative splicing profiles of MAP4K4 or SRSF3 were noted as well in brown adipocytes (BAs) with RNA-binding motif protein 4-knockout (RBM4−/−) compared to the wild-type counterparts. Moreover, the relatively high expressions of authentic SRSF3 transcripts encoding the splicing factor functioned as a novel regulator toward MAP4K4 splicing during brown adipogenesis. The presence of alternatively spliced MAP4K4 variants exerted differential effects on the phosphorylation of c-Jun N-terminal protein kinase (JNK) which was correlated with the differentiation or metabolic signature of BAs. Collectively, the RBM4-SRSF3-MAP4K4 splicing cascade constitutes a novel molecular mechanism in manipulating the development of BAs through related signaling pathways.
Structure-Based Classification and Anti-Cancer Effects of Plant Metabolites
Authors: Shin Seong-Ah , Moon Sun Young , Kim Woe-Yeon , Paek Seung-Mann , Park Hyun Ho , Lee Chang Sup
Published: September 2018

A variety of malignant cancers affect the global human population. Although a wide variety of approaches to cancer treatment have been studied and used clinically (surgery, radiotherapy, chemotherapy, and immunotherapy), the toxic side effects of cancer therapies have a negative impact on patients and impede progress in conquering cancer. Plant metabolites are emerging as new leads for anti-cancer drug development. This review summarizes these plant metabolites with regard to their structures and the types of cancer against which they show activity, organized by the organ or tissues in which each cancer forms. This information will be helpful for understanding the current state of knowledge of the anti-cancer effects of various plant metabolites against major types of cancer for the further development of novel anti-cancer drugs.
Belt-Driven Open Source Circuit Mill Using Low-Cost 3-D Printer Components
Authors: Oberloier Shane , Pearce Joshua M.
Published: September 2018

Barriers to inventing electronic devices involve challenges of iterating electronic designs due to long lead times for professional circuit board milling or high costs of commercial milling machines. To overcome these barriers, this study provides open source (OS) designs for a low-cost circuit milling machine. First, design modifications for mechanical and electrical subsystems of the OS Distributed 3-D (D3D) Robotics prototyping system are provided. Next, Copper Carve, an OS custom graphical user interface, is developed to enable circuit board milling by implementing backlash and substrate distortion compensation. The performance of the OS D3D circuit mill is then quantified and validated for: positional accuracy, cut quality, feature accuracy, and distortion compensation. Finally, the return on investment is calculated for inventors using it. The results show by properly compensating for motion inaccuracies with Copper Carve, the machine achieves a motion resolution of 10 microns, which is more than adequate for most circuit designs. The mill is at least five times less expensive than all commercial alternatives and the material costs of the D3D mill are repaid from fabricating 20–43 boards. The results show that the OS circuit mill is of high-enough quality to enable rapid invention and distributed manufacturing of complex products containing custom electronics.
PDMS-Based Microfluidic Devices for Cell Culture
Authors: Torino Stefania , Corrado Brunella , Iodice Mario , Coppola Giuseppe
Published: September 2018

Microfluidic technology has affirmed itself as a powerful tool in medical and biological research by offering the possibility of managing biological samples in tiny channels and chambers. Among the different applications, the use of microfluidics for cell cultures has attracted much interest from scientists worldwide. Traditional cell culture methods need high quantities of samples and reagents that are strongly reduced in miniaturized systems. In addition, the microenvironment is better controlled by scaling down. In this paper, we provide an overview of the aspects related to the design of a novel microfluidic culture chamber, the fabrication approach based on polydimethylsiloxane (PDMS) soft-lithography, and the most critical issues in shrinking the size of the system.
Association between Air Quality and Sedentary Time in 3270 Chinese Adults: Application of a Novel Technology for Posture Determination
Authors: Ma Yiqun , Yuan Bing , Fan Shuhui , Luo Yizhou , Wen Xu
Published: September 2018

This study investigated the association between ambient air quality and sedentary time in Chinese adults. The participants were 3270 Chinese users (2021 men and 1249 women) of wrist-worn activity trackers. The data of participants’ daily activities were collected from July 2015 to October 2015. A novel algorithm based on raw accelerometer data was employed to determine sedentary time. Personal data, including sex, age, weight and height, were self-reported by the participants. Data of air quality, ambient temperature and weather were collected from the data released by the China National Environmental Monitoring Centre and the China Central Meteorological Observatory and matched in accordance with the Global Positioning System and time information. Multilevel regression analyses were conducted to investigate the association between air quality and sedentary time and adjusted for gender, age, region, body mass index, weather, temperature, weekday/weekend and monitored wake time per day. Better air quality index levels and lower concentrations of fine particulate matter were significantly associated with approximately 20 and 45 min reduction in sedentary time, respectively. Poor air quality appears to be an independent factor associated with prolonged sedentary time in Chinese adults.
Modeling the Transport and Fate of Sediments Released from Marine Construction Projects in the Coastal Waters of British Columbia, Canada
Authors: Fissel David B. , Lin Yuehua
Published: September 2018

Major marine construction projects, resulting in the release of sediments, are subject to environmental assessment and other regulatory approval processes. An important tool used for this is the development of specialized numerical methods for these marine activities. An integrated set of numerical methods addresses four distinct topics: (1) The near-field release and mixing of suspended sediments into the water column (i.e., the initial dilution zone); (2) the transport of the suspended sediments under the influence of complex ocean currents in the far-field; (3) the settling of the transported suspended sediments onto the seabed; and (4) the potential for resuspension of the deposited sediments due to sporadic occurrences of unusually large near-bottom currents. A review of projects subjected to environmental assessment in the coastal waters of British Columbia, from the year 2006 to 2017, is presented to illustrate the numerical models being used and their ongoing development. Improvements include higher resolution model grids to better represent the near-field, the depiction of particle size dependent vertical settling rates and the computation of resuspension of initially deposited sediments, especially in relation to temporary subsea piles of sediments arising from trenching for marine pipelines. The ongoing challenges for this numerical modeling application area are also identified.
Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens
Authors: Champion Olivia L. , Titball Richard W. , Bates Steven
Published: September 2018

In the past decade, Galleria mellonella (wax moth) larvae have become widely used as a non-mammalian infection model. However, the full potential of this infection model has yet to be realised, limited by the variable quality of larvae used and the lack of standardised procedures. Here, we review larvae suitable for research, protocols for dosing larvae, and methods for scoring illness in larvae infected with fungal pathogens. The development of standardised protocols for carrying out our experimental work will allow high throughput screens to be developed, changing the way in which we evaluate panels of mutants and strains. It will also enable the in vivo screening of potential antimicrobials at an earlier stage in the research and development cycle.
Watery Entanglements in the Cypriot Hinterland
Authors: Steel Louise
Published: September 2018

This paper examines how water shaped people’s interaction with the landscape in Cyprus during the Bronze Age. The theoretical approach is drawn from the new materialisms, effectively a ‘turn to matter’, which emphasises the very materiality of the world and challenges the privileged position of human agents over the rest of the environment. The paper specifically moves away from more traditional approaches to landscape archaeology, such as central place theory and more recently network theory, which serve to separate and distance people from the physical world they live in, and indeed are a part of; instead, it focuses on an approach that embeds humans, and the social/material worlds they create, as part of the environment, exploring human interactions within the landscape as assemblages, or entanglements of matter. It specifically emphasises the materiality and agency of water and how this shaped people’s engagement with, and movement through, their landscape. The aim is to encourage archaeologists to engage with the materiality of things, to better understand how people and other matter co-create the material (including social) world.
Mechanistic Insight into Etching Chemistry and HF-Assisted Etching of MgO-Al2O3-SiO2 Glass-Ceramic
Authors: Ji Yanxin , Yang Shun , Li Zhulian , Duan Junjie , Xu Meng , Jiang Hong , Li Changjiu , Chen Yongjun
Published: September 2018

The present study focuses on the etching conditions and mechanism of MgO-Al2O3-SiO2 glass-ceramic (MAS) in hydrofluoric acid (HF). The results show that the amorphous phase has 218 times higher etching rate than pure cordierite crystal at room temperature. In addition, the activation energies of cordierite and amorphous phases in the HF solution are 52.5 and 30.6 kJ/mol, respectively. The time (tad) taken for complete dissolution of the amorphous phase depends on the HF concentration (CHF). Based on the etching experiments, a new model is established and refined to assess the tad evolution. In addition, a highly crystalline cordierite phase, with the high specific surface area (59.4 m2·g−1) and mesoporous structure, has been obtained by HF etching. This paper presents novel insights into the etching chemistry and opens up avenues for further research in the area of cordierite-based catalytic ceramics.
Operators of Fractional Calculus and Their Applications
Authors: Srivastava Hari Mohan
Published: September 2018

n/a
A Game-Theoretic Loss Allocation Approach in Power Distribution Systems with High Penetration of Distributed Generations
Authors: Pourahmadi Farzaneh , Dehghanian Payman
Published: September 2018

Allocation of the power losses to distributed generators and consumers has been a challenging concern for decades in restructured power systems. This paper proposes a promising approach for loss allocation in power distribution systems based on a cooperative concept of game-theory, named Shapley Value allocation. The proposed solution is a generic approach, applicable to both radial and meshed distribution systems as well as those with high penetration of renewables and DG units. With several different methods for distribution system loss allocation, the suggested method has been shown to be a straight-forward and efficient criterion for performance comparisons. The suggested loss allocation approach is numerically investigated, the results of which are presented for two distribution systems and its performance is compared with those obtained by other methodologies.
Fractional Queues with Catastrophes and Their Transient Behaviour
Authors: Ascione Giacomo , Leonenko Nikolai , Pirozzi Enrica
Published: September 2018

Starting from the definition of fractional M/M/1 queue given in the reference by Cahoy et al. in 2015 and M/M/1 queue with catastrophes given in the reference by Di Crescenzo et al. in 2003, we define and study a fractional M/M/1 queue with catastrophes. In particular, we focus our attention on the transient behaviour, in which the time-change plays a key role. We first specify the conditions for the global uniqueness of solutions of the corresponding linear fractional differential problem. Then, we provide an alternative expression for the transient distribution of the fractional M/M/1 model, the state probabilities for the fractional queue with catastrophes, the distributions of the busy period for fractional queues without and with catastrophes and, finally, the distribution of the time of the first occurrence of a catastrophe.
On Magnifying Elements in E-Preserving Partial Transformation Semigroups
Authors: Kaewnoi Thananya , Petapirak Montakarn , Chinram Ronnason
Published: September 2018

Let S be a semigroup. An element a of S is called a right [left] magnifying element if there exists a proper subset M of S satisfying S = M a [ S = a M ] . Let E be an equivalence relation on a nonempty set X. In this paper, we consider the semigroup P ( X , E ) consisting of all E-preserving partial transformations, which is a subsemigroup of the partial transformation semigroup P ( X ) . The main propose of this paper is to show the necessary and sufficient conditions for elements in P ( X , E ) to be right or left magnifying.
Clinical Safety of Combined Targeted and Viscum album L. Therapy in Oncological Patients
Authors: Thronicke Anja , Oei Shiao Li , Merkle Antje , Matthes Harald , Schad Friedemann
Published: September 2018

Background: Despite improvement of tumor response rates, targeted therapy may induce toxicities in cancer patients. Recent studies indicate amelioration of adverse events (AEs) by add-on mistletoe (Viscum album L., VA) in standard oncological treatment. The primary objective of this multicenter observational study was to determine the safety profile of targeted and add-on VA therapy compared to targeted therapy alone. Methods: Demographic and medical data were retrieved from the Network Oncology registry. Allocation to either control (targeted therapy) or combinational group (targeted/add-on VA) was performed. Safety-associated variables were evaluated by adjusted multivariable analyses. Results: The median age of the study population (n = 310) at first diagnosis was 59 years; 67.4% were female. In total, 126 patients (40.6%) were in the control and 184 patients (59.4%) in the combination group. Significant differences were observed between both groups with respect to overall AE frequency (χ2 = 4.1, p = 0.04) and to discontinuation of standard oncological treatment (χ2 = 4.8, p = 0.03) with lower rates in the combinational group (20.1%, 35% respectively) compared to control (30.2%, 60.5%, respectively). Addition of VA to targeted therapy significantly reduced the probability of oncological treatment discontinuation by 70% (Odds ratio (OR) 0.30, p = 0.02). Conclusions: Our results indicate a highly significant reduction of AE-induced treatment discontinuation in all-stage cancer patients when treated with VA in addition to targeted therapy.
Poly(1-trimethylsilyl-1-propyne)-Based Hybrid Membranes: Effects of Various Nanofillers and Feed Gas Humidity on CO2 Permeation
Authors: Dai Zhongde , Løining Vilde , Deng Jing , Ansaloni Luca , Deng Liyuan
Published: September 2018

Poly(1-trimethylsilyl-1-propyne) (PTMSP) is a high free volume polymer with exceptionally high gas permeation rate but the serious aging problem and low selectivity have limited its application as CO2 separation membrane material. Incorporating inorganic nanoparticles in polymeric membranes has been a common approach to improve the separation performance of membranes, which has also been used in PTMSP based membrane but mostly with respect to tackling the aging issues. Aiming at increasing the CO2 selectivity, in this work, hybrid membranes containing four types of selected nanofillers (from 0 to 3D) were fabricated using PTMSP as the polymer matrix. The effects of the various types of nanofillers on the CO2 separation performance of the resultant membranes were systematically investigated in humid conditions. The thermal, chemical and morphologic properties of the hybrid membranes were characterized using TGA, FTIR and SEM. The gas permeation properties of the hybrid membranes were evaluated using mixed gas permeation test with the presence of water vapour to simulate the flue gas conditions. Experiments show that the addition of different fillers results in significantly different separation performances; The addition of ZIF-L porous 2D filler improves the CO2/N2 selectivity at the expenses of CO2 permeability, while the addition of TiO2, ZIF-7 and ZIF-8 increases the CO2 permeability but the CO2/N2 selectivity decreases.
A Meta-Analysis of Intracortical Device Stiffness and Its Correlation with Histological Outcomes
Authors: Stiller Allison M. , Black Bryan J. , Kung Christopher , Ashok Aashika , Cogan Stuart F. , Varner Victor D. , Pancrazio Joseph J.
Published: September 2018

Neural implants offer solutions for a variety of clinical issues. While commercially available devices can record neural signals for short time periods, they fail to do so chronically, partially due to the sustained tissue response around the device. Our objective was to assess the correlation between device stiffness, a function of both material modulus and cross-sectional area, and the severity of immune response. Meta-analysis data were derived from nine previously published studies which reported device material and geometric properties, as well as histological outcomes. Device bending stiffness was calculated by treating the device shank as a cantilevered beam. Immune response was quantified through analysis of immunohistological images from each study, specifically looking at fluorescent markers for neuronal nuclei and astrocytes, to assess neuronal dieback and gliosis. Results demonstrate that the severity of the immune response, within the first 50 µm of the device, is highly correlated with device stiffness, as opposed to device modulus or cross-sectional area independently. In general, commercially available devices are around two to three orders of magnitude higher in stiffness than devices which induced a minimal tissue response. These results have implications for future device designs aiming to decrease chronic tissue response and achieve increased long-term functionality.
Atomistic Investigation of Anisotropic Nanoindentation Behavior of Nanotwinned Aluminum Containing Inclined Twin Boundaries
Authors: Liu Yuan , Duan Yanfeng , Zhang Junjie
Published: September 2018

Nanotwinned metals exhibit superior mechanical properties due to unique dislocation–twin boundary interactions. In the present work, we elucidate the microscopic deformation mechanisms and their correlations with the macroscopic mechanical response of nanotwinned Al containing inclined twin boundaries under nanoindentation by means of molecular dynamics simulations. The effect of twin boundary orientation with respect to the indented surface on the nanoindentation is evaluated. Simulation results reveal that dislocation slip, dislocation–twin boundary interaction, and twin boundary migration operate in parallel in the plastic deformation of nanotwinned Al. The inclination angle of twin boundaries with respect to indented surface has a strong influence on the interaction between individual deformation modes, which in turn leads to the anisotropic indentation behavior of nanotwinned Al.
Effect of Whey Protein Supplementation on Physical Performance and Body Composition in Army Initial Entry Training Soldiers
Authors: McAdam Jeremy S. , McGinnis Kaitlin D. , Beck Darren T. , Haun Cody T. , Romero Matthew A. , Mumford Petey W. , Roberson Paul A. , Young Kaelin C. , Lohse Keith R. , Lockwood Christopher M. , Roberts Michael D. , Sefton JoEllen M.
Published: September 2018

We investigated the effects of whey protein (WP) supplementation on body composition and physical performance in soldiers participating in Army Initial Entry Training (IET). Sixty-nine, male United States Army soldiers volunteered for supplementation with either twice daily whey protein (WP, 77 g/day protein, ~580 kcal/day; n = 34, age = 19 ± 1 year, height = 173 ± 6 cm, weight = 73.4 ± 12.7 kg) or energy-matched carbohydrate (CHO) drinks (CHO, 127 g/day carbohydrate, ~580 kcal/day; n = 35, age = 19 ± 1 year, height = 173 ± 5 cm, weight = 72.3 ± 10.9 kg) for eight weeks during IET. Physical performance was evaluated using the Army Physical Fitness Test during weeks two and eight. Body composition was assessed using 7-site skinfold assessment during weeks one and nine. Post-testing push-up performance averaged 7 repetitions higher in the WP compared to the CHO group (F = 10.1, p < 0.001) when controlling for baseline. There was a significant decrease in fat mass at post-training (F = 4.63, p = 0.04), but no significant change in run performance (F = 3.50, p = 0.065) or fat-free mass (F = 0.70, p = 0.41). Effect sizes for fat-free mass gains were large for both the WP (Cohen’s d = 0.44) and CHO (Cohen’s d = 0.42) groups. WP had a large effect on fat mass (FM) loss (Cohen’s d = −0.67), while CHO had a medium effect (Cohen’s d = −0.40). Twice daily supplementation with WP improved push-up performance and potentiated reductions in fat mass during IET training in comparison to CHO supplementation.
Changes in Plasma Acylcarnitine and Lysophosphatidylcholine Levels Following a High-Fructose Diet: A Targeted Metabolomics Study in Healthy Women
Authors: Gonzalez-Granda Anita , Damms-Machado Antje , Basrai Maryam , Bischoff Stephan C.
Published: September 2018

Background: The consumption of high amounts of fructose is associated with metabolic diseases. However, the underlying mechanisms are largely unknown. Objective: To determine the effects of high fructose intake on plasma metabolomics. Study design: We enrolled 12 healthy volunteers (six lean and six obese women, age 24–35 years) in a crossover intervention study. All participants carried out three diets: (1) low fructose (<10 g/day); (2) high fructose (100 g/day) from natural food sources (fruit); and (3) high fructose (100 g/day) from high fructose syrup (HFS). Outcome measures: The primary outcome was changes in plasma metabolites measured by targeted metabolomics. Results: High compared to low fructose diets caused a marked metabolite class separation, especially because of changes in acylcarnitine and lysophosphatidylcholine levels. Both high fructose diets resulted in a decrease in mean acylcarnitine levels in all subjects, and an increase in mean lysophosphatidylcholine and diacyl-phosphatidylcholine levels in obese individuals. Medium chain acylcarnitines were negatively correlated with serum levels of liver enzymes and with the fatty liver index. Discussion: The metabolic shifts induced by high fructose consumption suggest an inhibition of mitochondrial β-oxidation and an increase in lipid peroxidation. The effects tended to be more pronounced following the HFS than the fruit diet.
Oral Bioavailability and Lymphatic Transport of Pueraria Flavone-Loaded Self-Emulsifying Drug-Delivery Systems Containing Sodium Taurocholate in Rats
Authors: Qiao Jin , Ji Danyang , Sun Shilin , Zhang Guangyuan , Liu Xin , Sun Bingxue , Guan Qingxiang
Published: September 2018

We developed self-microemulsifying drug-delivery systems (SMEDDS), including bile salts, to improve the oral bioavailability of pueraria flavones (PFs). The physical properties of the SMEDDS using Cremophor RH 40, and bile salts as mixed surfactants at weight ratios of 10:0–0:10 were determined. The particle sizes of PFs-SMEDDSNR containing sodium taurocholate (NaTC) and Cremophor RH 40, and PFs-SMEDDSR containing Cremophor RH 40 were measured upon dilution with deionized water and other aqueous media. Dilution volume presented no remarkable effects on particle size, whereas dilution media slightly influenced particle size. PFs-SMEDDSNR and PFs-SMEDDSR provided similar release rates in pH-1.2 hydrochloride solution. However, the release rate of PFs-SMEDDSNR was faster than that of PFs-SMEDDSR in pH-6.8 phosphate buffer containing 20 mM NaTC and 500 U/mL porcine pancreas lipase. The pharmacokinetics and bioavailability were measured in rats. The oral bioavailability of PFs-SMEDDSNR was 2.57- and 2.28-fold that of a suspension of PFs (PFs-suspension) before and after the blockade of the lymphatic transport route by cycloheximide, respectively. These results suggested PFs-SMEDDSNR could significantly improve the oral relative absorption of PFs via the lymphatic uptake pathway. SMEDDS containing NaTC may provide an effective approach for enhancing the oral bioavailability of PFs.
Franz Diffusion Cell Approach for Pre-Formulation Characterisation of Ketoprofen Semi-Solid Dosage Forms
Authors: Salamanca Constain H. , Barrera-Ocampo Alvaro , Lasso Juan C. , Camacho Nathalia , Yarce Cristhian J.
Published: September 2018

This study aimed to evaluate and compare, using the methodology of Franz diffusion cells, the ketoprofen (KTP) releasing profiles of two formulations: A gel and a conventional suspension. The second aim was to show that this methodology might be easily applied for the development of semi-solid prototypes and claim proof in pre-formulation stages. Drug release analysis was carried out under physiological conditions (pH: 5.6 to 7.4; ionic strength 0.15 M; at 37 °C) for 24 h. Three independent vertical Franz cells were used with a nominal volume of the acceptor compartment of 125 mL and a diffusion area of 2.5 cm2. Additionally, two different membranes were evaluated: A generic type (regenerated cellulose) and a transdermal simulation type (Strat-M®). The KTP permeation profiles demonstrated that depending on the membrane type and the vehicle used, the permeation is strongly affected. High permeation efficiencies were obtained for the gel formulation, and the opposite effect was observed for the suspension formulation. Moreover, the permeation studies using Strat-M membranes represent a reproducible methodology, which is easy to implement for pre-formulation stage or performance evaluation of semi-solid pharmaceutical products for topical or transdermal administration.
Preparation and Properties of sc-PLA/PMMA Transparent Nanofiber Air Filter
Authors: Lv Shengnan , Zhao Xin , Shi Lei , Zhang Gaokai , Wang Shubo , Kang Weimin , Zhuang Xupin
Published: September 2018

Particulate matter (PM) pollution is a serious concern for the environment and public health. To protect indoor air quality, nanofiber filters have been used to coat window screens due to their high PM removal efficiency, transparency and low air resistance. However, these materials have poor mechanical property. In this study, electrostatic induction-assisted solution blowing was used to fabricate polylactide stereocomplex (sc-PLA), which served as reinforcement to enhance the physical cross-linking point to significantly restrict poly(methyl methacrylate) (PMMA) molecular chain motion and improve the mechanical properties of sc-PLA/PMMA nanofibers. Moreover, the introduction of sc-PLA led to the formation of thick/thin composite nanofiber structure, which is beneficial for the mechanical property. Thus, sc-PLA/PMMA air filters of ~83% transparency with 99.5% PM2.5 removal and 140% increase in mechanical properties were achieved when 5 wt % sc-PLA was added to PMMA. Hence, the addition of sc-PLA to transparent filters can effectively improve their performance.
Fabrication of PPy Nanosphere/rGO Composites via a Facile Self-Assembly Strategy for Durable Microwave Absorption
Authors: Wang Ying , Du Yunchen , Wu Bo , Han Binhua , Dong Shaoming , Han Xijiang , Xu Ping
Published: September 2018

Traditional magnetic metal and alloy materials suffer from easy oxidation and high density, which hinders their practical application as high-performance microwave absorbers. Lightweight and durability have become new goals in the fabrication of the next generation of microwave absorbers. Herein, we report the synthesis of polypyrrole (PPy) nanosphere/reduced graphene oxide (rGO) composites through chemical reduction of self-assembly PPy nanosphere/GO hybrids. PPy nanospheres and GO are integrated effectively by π–π interaction of dual conjugated systems. When the mass ratio of PPy nanospheres to rGO is 0.6:1, the resultant composite, PPy/rGO-0.6, presents comparable/superior reflection loss characteristics to those magnetic metals and their related graphene-based composites in previous studies. Electromagnetic analysis reveals that well-matched characteristic impedance, multiple polarization loss, and good conductivity loss are, together, responsible for the excellent microwave absorption performance of PPy/rGO-0.6. More importantly, PPy/rGO-0.6 also exhibits good microwave absorption after being treated at 423 K for a long time. This work provides a new idea for designing and preparing a high-performance microwave absorber with lightweight and durable features.
Mathematical Modeling of RBC Count Dynamics after Blood Loss
Authors: Tetschke Manuel , Lilienthal Patrick , Pottgiesser Torben , Fischer Thomas , Schalk Enrico , Sager Sebastian
Published: September 2018

The regeneration of red blood cells (RBCs) after blood loss is an individual complex process. We present a novel simple compartment model which is able to capture the most important features and can be personalized using parameter estimation. We compare predictions of the proposed and personalized model to a more sophisticated state-of-the-art model for erythropoiesis, and to clinical data from healthy subjects. We discuss the choice of model parameters with respect to identifiability. We give an outlook on how extensions of this novel mathematical model could have an important impact for personalized clinical decision support in the case of polycythemia vera (PV). PV is a slow-growing type of blood cancer, where especially the production of RBCs is increased. The principal treatment targeting the symptoms of PV is bloodletting (phlebotomy), at regular intervals that are based on personal experiences of the physicians. Model-based decision support might help to identify optimal and individualized phlebotomy schedules.
The Seepage Control of the Tunnel Excavated in High-Pressure Water Condition Using Multiple Times Grouting Method
Authors: Gong Bin , Jiang Yujing , Okatsu Keisuke , Wu Xuezhen , Teduka Jin , Aoki Koichi
Published: September 2018

Groundwater can cause many hazardous problems when a tunnel is excavating. Seepage force acting on the support structure and the tunnel surface cannot be negligible. Under high groundwater table condition, the seepage situation becomes more complex and it is more difficult to control the leakage of groundwater to flow into a tunnel. In the paper, a multiple times grouting method is proposed, and the mechanical deformation behavior of surrounding rock is analyzed using the FLAC3D (Fast Lagrangian Analysis of Continua in 3 Dimensions) software according to the high groundwater table condition of the Hokusatsu tunnel. The results present that multiple times grouting can control leakage and the rock deformation well, compared with one-time grouting condition in rock breaking and high water pressure area. The seepage force decrease around the tunnel and the displacement is controlled effectively. The pore pressure reduces inside the grouting zone using a new kind of grouting material, which is high permeability ultramicro particle cement (average particle size 1.5 μm). In the test fieldwork, the grouting scheme reduces the maximum discharge from 300 t/h to 40 t/h, and there is not obvious deformation and abnormal stress in the tunnel. The multiple times grouting method proposed in this research is verified effectively and can supply a positive experience to on-site construction.