bluuebunny/crossref_metadata_2025 huggingface dataset:
A Catastrophic Biodiversity Loss in the Environment Is Being Replicated on the Skin Microbiome: Is This a Major Contributor to the Chronic Disease Epidemic?
Authors: Wallen-Russell Christopher , Pearlman Nancy , Wallen-Russell Samuel , Cretoiu Dragos , Thompson Dana Claudia , Voinea Silviu Cristian
Published: November 2023

There has been a catastrophic loss of biodiversity in ecosystems across the world. A similar crisis has been observed in the human gut microbiome, which has been linked to “all human diseases affecting westernized countries”. This is of great importance because chronic diseases are the leading cause of death worldwide and make up 90% of America’s healthcare costs. Disease development is complex and multifactorial, but there is one part of the body’s interlinked ecosystem that is often overlooked in discussions about whole-body health, and that is the skin microbiome. This is despite it being a crucial part of the immune, endocrine, and nervous systems and being continuously exposed to environmental stressors. Here we show that a parallel biodiversity loss of 30–84% has occurred on the skin of people in the developed world compared to our ancestors. Research has shown that dysbiosis of the skin microbiome has been linked to many common skin diseases and, more recently, that it could even play an active role in the development of a growing number of whole-body health problems, such as food allergies, asthma, cardiovascular diseases, and Parkinson’s, traditionally thought unrelated to the skin. Damaged skin is now known to induce systemic inflammation, which is involved in many chronic diseases. We highlight that biodiversity loss is not only a common finding in dysbiotic ecosystems but also a type of dysbiosis. As a result, we make the case that biodiversity loss in the skin microbiome is a major contributor to the chronic disease epidemic. The link between biodiversity loss and dysbiosis forms the basis of this paper’s focus on the subject. The key to understanding why biodiversity loss creates an unhealthy system could be highlighted by complex physics. We introduce entropy to help understand why biodiversity has been linked with ecosystem health and stability. Meanwhile, we also introduce ecosystems as being governed by “non-linear physics” principles—including chaos theory—which suggests that every individual part of any system is intrinsically linked and implies any disruption to a small part of the system (skin) could have a significant and unknown effect on overall system health (whole-body health). Recognizing the link between ecosystem health and human health allows us to understand how crucial it could be to maintain biodiversity across systems everywhere, from the macro-environment we inhabit right down to our body’s microbiome. Further, in-depth research is needed so we can aid in the treatment of chronic diseases and potentially change how we think about our health. With millions of people currently suffering, research to help mitigate the crisis is of vital importance.
Symbiotic Functioning and Photosynthetic Rates Induced by Rhizobia Associated with Jack Bean (Canavalia ensiformis L.) Nodulation in Eswatini
Authors: Ngwenya Zanele D. , Dakora Felix D.
Published: November 2023

Improving the efficiency of the legume–rhizobia symbiosis in African soils for increased grain yield would require the use of highly effective strains capable of nodulating a wide range of legume plants. This study assessed the photosynthetic functioning, N2 fixation, relative symbiotic effectiveness (%RSE) and C assimilation of 22 jack bean (Canavalia ensiformis L.) microsymbionts in Eswatini soils as a first step to identifying superior isolates for inoculant production. The results showed variable nodule number, nodule dry matter, shoot biomass and photosynthetic rates among the strains tested under glasshouse conditions. Both symbiotic parameters and C accumulation differed among the test isolates at the shoot, root and whole-plant levels. Although 7 of the 22 jack bean isolates showed much greater relative symbiotic efficiency than the commercial Bradyrhizobium strain XS21, only one isolate (TUTCEeS2) was statistically superior to the inoculant strain, which indicates its potential for use in inoculant formulation after field testing. Furthermore, the isolates that recorded high %RSE elicited greater amounts of fixed N.
Differentiating between Monofloral Portuguese Bee Pollens Using Phenolic and Volatile Profiles and Their Impact on Bioactive Properties
Authors: Larbi Samar , Aylanc Volkan , Rodríguez-Flores Maria Shantal , Calhelha Ricardo C. , Barros Lillian , Rezouga Feriel , Seijo Maria Carmen , Falcão Soraia I. , Vilas-Boas Miguel
Published: November 2023

Nowadays, bee products are commended by consumers for their medicinal and dietary properties. This study aimed to differentiate between monofloral bee pollens originating from Portugal using phenolic and volatile profiles and investigate their antioxidant and cytotoxic activity. Total phenolic and flavonoid compounds were recorded between 2.9–35.8 mg GAE/g and 0.7–4.8 mg QE/g, respectively. The LC/DAD/ESI-MSn analytical results allowed us to identify and quantify a total of 72 compounds, including phenolic and phenylamide compounds, whereas GC-MS results revealed the presence of 49 different compounds, mostly ketones, aldehydes, esters, hydrocarbons, and terpenes. The highest DPPH• radical scavenging activity, EC50: 0.07 mg/mL, was recorded in the sample dominated by Castanae sp. pollen, whereas the Rubus sp. (1.59 mM Trolox/mg) and Cistaceae sp. (0.09 mg GAE/g) pollen species exhibited the highest antioxidant activity in ABTS•+ and reducing power assays, respectively. Regarding the anti-carcinogenic activity, only Carduus sp. showed remarkable cytotoxic potential against MCF-7.
Optimization of Chemical Extraction Conditions of Dietary Fiber from Cistanche deserticola Residues and Its Structural Characteristics and Physicochemical and Functional Properties
Authors: Sun Ziming , Zhao Yuanyuan , Zhang Zhen , Wang Li , Du Jianming , Zhang Shengxiang
Published: November 2023

Cistanche deserticola residues are by-products of the industrial production of Cistanche deserticola, which are currently often discarded, resulting in the waste of resources. In order to achieve the efficient utilization of Cistanche deserticola, dietary fiber from Cistanche deserticola residues was extracted chemically and the optimization of the extraction conditions was performed, using the response surface methodology to study the effects of the NaOH concentration, extraction temperature, extraction time, and solid–liquid ratio on the yield of water-soluble dietary fiber (SDF). The structural, physicochemical, and functional properties of the dietary fiber were also investigated. The results showed that the optimal conditions were as follows: NaOH concentration of 3.7%, extraction temperature of 71.7 °C, extraction time of 89.5 min, and solid–liquid ratio of 1:34. The average yield of SDF was 19.56%, which was close to the predicted value of 19.66%. The two dietary fiber types had typical polysaccharide absorption peaks and typical type I cellulose crystal structures, and the surface microstructures of the two dietary fiber types were different, with the surface of SDF being looser and more porous. Both dietary fiber types had good functional properties, with SDF having the strongest water-holding capacity and the strongest adsorption capacity for nitrite, cholesterol, sodium cholate, and glucose, while IDF had a better oil-holding capacity. These results suggest that Cistanche deserticola residues are a good source of dietary fiber and have promising applications in the functional food processing industry.
Hepatoprotective Effects of Biochanin A on Thioacetamide-Induced Liver Cirrhosis in Experimental Rats
Authors: Ibrahim Mohamed Yousif , Alamri Zaenah Zuhair , Juma Ameena S. M. , Hamood Sarah Ashour , Shareef Suhayla Hamad , Abdulla Mahmood Ameen , Jayash Soher Nagi
Published: November 2023

The protective effect of biochanin A (BCA) on the histopathology, immunohistochemistry, and biochemistry of thioacetamide (TAA)-induced liver cirrhosis in vivo was investigated. There was a significant reduction in liver weight and hepatocyte propagation, with much lower cell injury in rat groups treated with BCA (25 mg/kg and 50 mg/kg) following a TAA induction. These groups had significantly lower levels of proliferating cell nuclear antigen (PCNA) and α-smooth muscle actin (α-SMA). The liver homogenates showed increased antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as decreased malondialdehyde (MDA) levels. The serum biomarkers associated with liver function, namely alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma glutamyl transaminase (GGT), returned to normal levels, comparable to those observed in both the normal control group and the reference control group. Taken together, the normal microanatomy of hepatocytes, the inhibition of PCNA and α-SMA, improved antioxidant enzymes (SOD, CAT, and GPx), and condensed MDA with repairs of liver biomarkers validated BCA’s hepatoprotective effect.
A Muti-Substrate Flavonol O-glucosyltransferases from Safflower
Authors: Qi Shuyi , He Beixuan , Wang Haotian , Duan Yaqian , Wang Lunuan , Gao Yue , Guo Meili
Published: November 2023

To explore the complete biosynthesis process of flavonoid glycosides in safflower, specifically the key glycosyltransferase that might be involved, as well as to develop an efficient biocatalyst to synthesize flavonoid glycosides, a glycosyltransferase CtUGT4, with flavonoid-O-glycosyltransferase activity, was identified in safflower. The fusion protein of CtUGT4 was heterologously expressed in Escherichia coli, and the target protein was purified. The recombinant protein can catalyze quercetin to form quercetin-7-O-glucoside, and kaempferol to form kaempferol-3-O in vitro, and a series of flavones, flavonols, dihydroflavones, chalcones, and chalcone glycosides were used as substrates to generate new products. CtUGT4 was expressed in the tobacco transient expression system, and the enzyme activity results showed that it could catalyze kaempferol to kaempferol-3-O-glucoside, and quercetin to quercetin-3-O-glucoside. After overexpressing CtUGT4 in safflower, the content of quercetin-3-O-rutinoside in the safflower florets increased significantly, and the content of quercetin-3-O-glucoside also tended to increase, which preliminarily confirmed the function of CtUGT4 flavonoid-O-glycosyltransferase. This work demonstrated the flavonoid-O-glycosyltransferase function of safflower CtUGT4 and showed differences in the affinity for different flavonoid substrates and the regioselectivity of catalytic sites in safflower, both in vivo and in vitro, providing clues for further research regarding the function of UGT genes, as well as new ideas for the cultivation engineering of the directional improvement of effective metabolites in safflower.
Anti-Inflammatory Properties of Eugenol in Lipopolysaccharide-Induced Macrophages and Its Role in Preventing β-Cell Dedifferentiation and Loss Induced by High Glucose-High Lipid Conditions
Authors: Gojani Esmaeel Ghasemi , Wang Bo , Li Dong-Ping , Kovalchuk Olga , Kovalchuk Igor
Published: November 2023

Inflammation is a natural immune response to injury, infection, or tissue damage. It plays a crucial role in maintaining overall health and promoting healing. However, when inflammation becomes chronic and uncontrolled, it can contribute to the development of various inflammatory conditions, including type 2 diabetes. In type 2 diabetes, pancreatic β-cells have to overwork and the continuous impact of a high glucose, high lipid (HG-HL) diet contributes to their loss and dedifferentiation. This study aimed to investigate the anti-inflammatory effects of eugenol and its impact on the loss and dedifferentiation of β-cells. THP-1 macrophages were pretreated with eugenol for one hour and then exposed to lipopolysaccharide (LPS) for three hours to induce inflammation. Additionally, the second phase of NLRP3 inflammasome activation was induced by incubating the LPS-stimulated cells with adenosine triphosphate (ATP) for 30 min. The results showed that eugenol reduced the expression of proinflammatory genes, such as IL-1β, IL-6 and cyclooxygenase-2 (COX-2), potentially by inhibiting the activation of transcription factors NF-κB and TYK2. Eugenol also demonstrated inhibitory effects on the levels of NLRP3 mRNA and protein and Pannexin-1 (PANX-1) activation, eventually impacting the assembly of the NLRP3 inflammasome and the production of mature IL-1β. Additionally, eugenol reduced the elevated levels of adenosine deaminase acting on RNA 1 (ADAR1) transcript, suggesting its role in post-transcriptional mechanisms that regulate inflammatory responses. Furthermore, eugenol effectively decreased the loss of β-cells in response to HG-HL, likely by mitigating apoptosis. It also showed promise in suppressing HG-HL-induced β-cell dedifferentiation by restoring β-cell-specific biomarkers. Further research on eugenol and its mechanisms of action could lead to the development of therapeutic interventions for inflammatory disorders and the preservation of β-cell function in the context of type 2 diabetes.
Nanocatalysis for Environmental Protection, Energy, and Green Chemistry
Authors: Panagiotopoulou Paraskevi , Yentekakis Ioannis V.
Published: November 2023

Nowadays, nanoscience and nanotechnology depict cutting-edge areas of modern science and technology across an array of applications, including heterogeneous catalysis [...]
Nutritional Approach in Selected Inherited Metabolic Cardiac Disorders—A Concise Summary of Available Scientific Evidence
Authors: Luca Alina Costina , Pădureț Ioana-Alexandra , Țarcă Viorel , David Simona Georgiana , Mîndru Dana Elena , Roșu Solange Tamara , Roșu Eduard Vasile , Adumitrăchioaiei Heidrun , Bernic Jana , Cojocaru Elena , Țarcă Elena
Published: November 2023

Inborn errors of metabolism (IMDs) are a group of inherited diseases that manifest themselves through a myriad of signs and symptoms, including structural or functional cardiovascular damage. The therapy of these diseases is currently based on enzyme-replacement therapy, chaperone therapy or the administration of supplements and the establishment of personalized dietary plans. Starting from the major signs identified by the pediatric cardiologist that can indicate the presence of such a metabolic disease—cardiomyopathies, conduction disorders or valvular dysplasias—we tried to paint the portrait of dietary interventions that can improve the course of patients with mitochondrial diseases or lysosomal abnormalities. The choice of the two categories of inborn errors of metabolism is not accidental and reflects the experience and concern of the authors regarding the management of patients with such diagnoses. A ketogenic diet offers promising results in selected cases, although, to date, studies have failed to bring enough evidence to support generalized recommendations. Other diets have been successfully utilized in patients with IMDs, but their specific effect on the cardiac phenotype and function is not yet fully understood. Significant prospective studies are necessary in order to understand and establish which diet best suits every patient depending on the inherited metabolic disorder. The most suitable imagistic monitoring method for the impact of different diets on the cardiovascular system is still under debate, with no protocols yet available. Echocardiography is readily available in most hospital settings and brings important information regarding the impact of diets on the left ventricular parameters. Cardiac MRI (magnetic resonance imaging) could better characterize the cardiac tissue and bring forth both functional and structural information.
The Detection of SARS-CoV-2 Antibodies in an Exposed Human Population Is Biased by the Immunoassay Used: Implications in Serosurveillance
Authors: Llorente Francisco , Pérez-Ramírez Elisa , Pérez-Olmeda Mayte , Dafouz-Bustos Desirée , Fernández-Pinero Jovita , Martínez-Cortés Mercedes , Jiménez-Clavero Miguel Ángel
Published: November 2023

The presence of SARS-CoV-2 antibodies was examined over 7 months in a population of essential service workers exposed during the first epidemic wave in Madrid (Spain). Results obtained with different serological assays were compared. Firstly, serum samples obtained in April 2020 were analyzed using eleven SARS-CoV-2 antibody detection methods, including seven ELISAs, two CLIAs and two LFAs. While all of the ELISA tests and the Roche eCLIA method showed good performance, it was poorer for the Abbott CLIA and LFA tests. Sera from 115 workers with serologically positive results in April were collected 2 and 7 months after the first sampling and were analyzed using five of the tests previously assessed. The results showed that while some ELISA tests consistently detected the presence of anti-SARS-CoV-2 antibodies even 7 months after first detection, other methods, such as the Abbott CLIA test, showed an important reduction in sensitivity for these mature antibodies. The sensitivity increased after establishing new cut-off values, calculated taking into account both recent and old infections, suggesting that an adjustment of assay parameters may improve the detection of individuals exposed to the infection.
Advanced Progress in the Role of Adipose-Derived Mesenchymal Stromal/Stem Cells in the Application of Central Nervous System Disorders
Authors: Wu Haiyue , Fan Yishu , Zhang Mengqi
Published: November 2023

Currently, adipose-derived mesenchymal stromal/stem cells (ADMSCs) are recognized as a highly promising material for stem cell therapy due to their accessibility and safety. Given the frequently irreversible damage to neural cells associated with CNS disorders, ADMSC-related therapy, which primarily encompasses ADMSC transplantation and injection with exosomes derived from ADMSCs or secretome, has the capability to inhibit inflammatory response and neuronal apoptosis, promote neural regeneration, as well as modulate immune responses, holding potential as a comprehensive approach to treat CNS disorders and improve prognosis. Empirical evidence from both experiments and clinical trials convincingly demonstrates the satisfactory safety and efficacy of ADMSC-related therapies. This review provides a systematic summary of the role of ADMSCs in the treatment of central nervous system (CNS) disorders and explores their therapeutic potential for clinical application. ADMSC-related therapy offers a promising avenue to mitigate damage and enhance neurological function in central nervous system (CNS) disorders. However, further research is necessary to establish the safety and efficacy of clinical ADMSC-based therapy, optimize targeting accuracy, and refine delivery approaches for practical applications.
Exploration of Illicit Drug Detection Based on Goos–Hänchen Shift
Authors: Wang Yan , Zhou Xiaodi , Fan Xinmin , Huang Xiaodong , Zhang Lujun , Wang Chunyan
Published: November 2023

Amidst the escalating issue of drug abuse, an urgent need for effective illicit drug detection methods has arisen. This paper introduces a novel optical approach utilizing the Goos–Hänchen Shift (GHS) to explore the possibility of on-site rapid detection of illicit drugs. Delving into the mechanisms, light absorption and attenuation in biological samples are considered through absorption and attenuation coefficients, establishing connections between complex refractive indices, complex dielectric constants, and GHS. A self-assembled GHS detection system measured GHS values across various samples: ultrapure water, serum, methamphetamine, serum–methamphetamine, heroin, and serum–heroin. These experiments unveiled substantial GHS variations among the samples. Refractive indices for serum, serum–methamphetamine, and serum–heroin samples were computed using GHS values and sample extinction coefficients, highlighting GHS’s remarkable sensitivity to refractive index variations as a high-sensitivity refractive index sensing technology. The correlation between the dielectric constant and GHS was explored, yielding refractive indices for pure solutes—serum, methamphetamine, and heroin—of 1.66300, 1.51300, and 1.62300, respectively. Notably, the dielectric constants for these solutes were 2.76557, 2.28917, and 2.63413, emphasizing the dielectric constant’s discriminative potential in identifying illicit drugs. In conclusion, these findings suggest that GHS holds promise for distinguishing various illicit drug types, charting an innovative path for illicit drug detection.
Phytochemical Profiling and Biological Activities of Quercus sp. Galls (Oak Galls): A Systematic Review of Studies Published in the Last 5 Years
Authors: Banc Roxana , Rusu Marius Emil , Filip Lorena , Popa Daniela-Saveta
Published: November 2023

Quercus species have been widely used in traditional medicine, and recently, researchers’ attention has focused on galls of the genus Quercus as a source of health-promoting phytochemicals. This review presents a summary of the most recent findings on the phytochemistry and bioactivity of oak galls, following the screening of scientific papers published in two relevant databases, PubMed and Embase, between January 2018 and June 2023. The oak galls are rich in active compounds, mostly gallotannins and phenolic acids. Due to these secondary metabolites, the reviewed studies have demonstrated a wide range of biological activities, including antioxidant and anti-inflammatory actions, antimicrobial properties, tissue-protective effects, and antitumor, anti-aging, and hypoglycemic potential. Thus, oak galls are a promising natural matrix, to be considered in obtaining pharmaceutical and cosmetic preparations used in anti-aging strategies and, together with medications, in the management of age-related diseases. In further evaluations, the valuable functional properties of oak galls, reported mostly in preclinical studies, should be confirmed with clinical studies that would also take into account the potential health risks of their use.
A Critical Appraisal of the Most Recent Investigations on Ora-Pro-Nobis (Pereskia sp.): Economical, Botanical, Phytochemical, Nutritional, and Ethnopharmacological Aspects
Authors: Teixeira Valéria Maria Costa , Oliveira Anielle de , Backes Emanueli , Souza Cristina Giatti Marques de , Castoldi Rafael , Sá-Nakanishi Anacharis Babeto de , Bracht Lívia , Comar Jurandir Fernando , Corrêa Rúbia Carvalho Gomes , Leimann Fernanda Vitória , Bracht Adelar , Peralta Rosane Marina
Published: November 2023

Pereskia aculeata Miller and Pereskia grandfolia Haw, known as ‘ora-pro-nobis’, are unconventional vegetables belonging to the Cactaceae family, native to the Americas and common in the northeast and southeast regions of Brazil. This review attempts to present a balanced account of both the methods used for obtaining extracts from the diverse parts of the plants and the results that were obtained in terms of their applicability to foods and other products with biological activities. Attention will also be devoted to the properties of their bioactives and their applications to real food products. Methods for obtaining extracts from the diverse parts of the plants will be analyzed, as well as the chemical nature of the bioactives that were hitherto identified. Next, the applicability of ora-pro-nobis in either its integral form or in the form of extracts or other products (mucilages) to the production of food and dietary supplements will be analyzed. The species have been extensively investigated during the last few decades. But, the determination of chemical structures is frequently incomplete and there is a need for new studies on texture determination and color evaluation. Further studies exploring the fruit and flowers of P. aculeata are also required.
Comparison of Silk Hydrogels Prepared via Different Methods
Authors: Hua Jiahui , Huang Renyan , Huang Ying , Yan Shuqin , Zhang Qiang
Published: November 2023

Silk fibroin (SF) hydrogels have garnered extensive attention in biomedical materials, owing to their superior biological properties. However, the challenges facing the targeted silk fibroin hydrogels involve chemical agents and shortfalls in performance. In this study, the silk fibroin hydrogels were prepared in different ways: sonication induction, chemical crosslinking, photopolymerization, and enzyme-catalyzed crosslinking. The SF hydrogels derived from photopolymerization exhibited higher compressive properties, with 124 Kpa fracture compressive stress and breaks at about 46% compression. The chemical crosslinking and enzyme-catalyzed silk fibroin hydrogels showed superior toughness, yet sonication-induced hydrogels showed brittle performance resulting from an increase in silk II crystals. The chemical-crosslinked hydrogel demonstrated lower thermostability due to the weaker crosslinking degree. In vitro, all silk fibroin hydrogels supported the growth of human umbilical vein endothelial cells, as the cell viability of hydrogels without chemical agents was relatively higher. This study provides insights into the formation process of silk fibroin hydrogels and optimizes their design strategy for biomedical applications.
Sunflower and Watermelon Seeds and Their Hybrids with Pineapple Leaf Fibers as New Novel Thermal Insulation and Sound-Absorbing Materials
Authors: Ali Mohamed , Al-Suhaibani Zeyad , Almuzaiqer Redhwan , Al-Salem Khaled , Nuhait Abdullah , Algubllan Fahad , Al-Howaish Meshari , Aloraini Abdullah , Alqahtani Ibrahim
Published: November 2023

Pineapple leaf fiber (PALF), striped sunflower seed fiber (SFSF), and watermelon seed (WMS) are considered natural waste polymer materials, which are biodegradable and sustainable. This study presents new novel thermal insulation and sound absorption materials using such waste as raw materials. PALF, SFSF, and WMS were used as loose, bound, and hybrid samples with different compositions to develop promising thermal insulation and sound-absorbing materials. Eleven sample boards were prepared: three were loose, three were bound, and five were hybrid between PALF with either SFSF or WMS. Wood adhesive was used as a binder for both the bound and hybrid sample boards. Laboratory scale sample boards of size 30 cm × 30 cm with variable thicknesses were prepared. The results show that the average thermal conductivity coefficient for the loose samples at the temperature range 20–80 °C is 0.04694 W/(m.K), 0.05611 W/(m.K), and 0.05976 W/m.K for PALF, SFSF, and WMS, respectively. Those for bound sample boards are 0.06344 W/(m.K), 0.07113 W/(m.K), and 0.08344 W/m.K for PALF, SFSF, and WMS, respectively. The hybrid ones between PALF and SFSF have 0.05921 W/m.K and 0.06845 W/(m.K) for two different compositions. The other hybrid between PALF and WMS has 0.06577 W/(m.K) and 0.07007 for two different compassions. The sound absorption coefficient for most of the bound and hybrid boards is above 0.5 and reaches higher values at some different frequencies. The thermogravimetric analysis for both SFSF and WMS shows that they are thermally stable up to 261 °C and 270 °C, respectively. The three-point bending moment test was also performed to test the mechanical properties of the bound and hybrid sample boards. It should be mentioned that using such waste materials as new sources of thermal insulation and sound absorption materials in buildings and other applications would lead the world to utilize the waste until zero agrowaste is reached, which will lower the environmental impact.
Impact of Incorporating Free Calcium and Magnesium on the Heat Stability of a Dairy- and Soy-Protein-Containing Model Emulsion
Authors: Wang Wei , Tan Kevin Wei Jie , Chiang Poh Leong , Wong Wai Xin , Chen Wenpu , Lin Qi
Published: November 2023

This study investigated the impact of calcium chloride (CaCl2) and magnesium chloride (MgCl2) at varying concentrations on a model milk formulation’s physical and chemical properties after thermal treatment. The model milk was subjected to two-stage homogenization and pasteurization before being supplemented with different concentrations of CaCl2 or MgCl2. The findings revealed that elevating the concentration of either calcium or magnesium resulted in the milk emulsion having a higher viscosity and median particle size following heating. CaCl2 had a slightly stronger impact than MgCl2, particularly at higher concentrations. The milk samples also exhibited a reduction in the zeta potential as the ionic strength of the salt solution increased, with the CaCl2-fortified milk displaying a slightly lower negative surface charge than the MgCl2-fortified milk at the same dose. The model milk’s viscosity was evaluated after adding various salt concentrations and a temperature ramp from 20 to 80 °C. Notably, the viscosity and particle size changes demonstrated a non-linear relationship with increasing mineral levels, where a significant increase was observed at or above 5.0 mM. An emulsion stability analysis also revealed that the de-stabilization pattern of the high salt concentration sample differed significantly from its low salt concentration counterparts. These findings could serve as a basis for the future development of fortified UHT milk with nutritionally beneficial calcium and magnesium in industrial applications.
Enhanced Low-Density Silicone Foams Blown by Water–Hydroxyl Blends
Authors: Rebane Ingrid , Levin Karl Jakob , Mäeorg Uno , Johanson Urmas , Piirimägi Peeter , Tätte Tauri , Tamm Tarmo
Published: November 2023

Water, alcohols, diols, and glycerol are low-cost blowing agents that can be used to create the desired silicone foam structures. Although their combined use can be beneficial, it remains unclear how it affects the physical properties of the resulting materials. We conducted a comparative study of these hydroxyl-bearing blowing agents in fumed silica- and mica-filled polymer composite systems for simultaneous blowing and crosslinking to obtain a low-density, uniform porosity and superior mechanical properties. The foams were optimized for a uniform open-pore structure with densities ranging from 75 to 150 kg‧m−3. Varying the diol chain length (Cn) from one to seven carbons can alter the foam density and structure, thereby enhancing the foam tensile strength while maintaining a low density. Replacing 10 mol% of water with 1,4-butanediol decreased the density by 26%, while increasing the specific strength by 5%. By combining glycerol and water blowing, the resulting foams exhibited a 30% lower apparent density than their water-blown analogs. The results further showed that Cn > 4 alkane chain diols had an odd–even effect on the apparent density and cell wall thickness. All foamable compositions had viscosities of approximately 7000 cSt and curing times below 2 min, allowing for quick dispensing and sufficient time for the foam to cure in semi-industrial volumes.
3D Printed, Single-Use Bioreactor with Integrated Inline Sensors for Microbial and Mammalian Cell Cultivation—A Case Study
Authors: Schneider Samuel Lukas , Seidel Stefan , Seefeldt Andressa , Romang Michael , Kastl Simon , Gensel Julia , Neumeyer Thomas , John Gernot Thomas , Eibl Dieter
Published: November 2023

The development of upstream bioprocesses necessitates small, instrumented bioreactors for investigating and optimizing production processes in a cost-effective manner. Due to advances in both the equipment and the materials used in additive manufacturing, 3D printing of customized bioreactors is now in the realm of possibilities. In this study, a small-scale 3D printed bioreactor suitable for mammalian and microbial cultivations was developed, featuring a working volume of 90 mL, inline pH and dissolved oxygen probes and a levitating magnetic stirrer. Aeration channels and a sampling port were printed directly into the vessel walls. Additionally, the vessel was equipped with a 3D printed customizable optical biomass-sensor. The bioreactor’s performance was evaluated through technical characterization and proof of concept cultivations, demonstrating that mixing time and oxygen mass transfer were sufficient for cultivating mammalian as well as microbial cells at high cell densities. Specifically, an Escherichia coli fed-batch cultivation achieved a maximum OD600 of 204. Furthermore, a fed-batch cultivation of an IgG antibody-producing Chinese hamster ovary cell line reached a peak viable cell density of 10.2 × 106 cells mL−1 and a maximum product titer of 2.75 g L−1. Using a three-parameter fit, the inline biomass signal could be correlated to the corresponding offline values with satisfactory accuracy, making it possible to monitor cell growth in real-time.
Effects of MgO and Rare-Earth Oxides (Y2O3, Yb2O3, Dy2O3) on the Structural Characteristics and Electrical Properties of BaTiO3
Authors: Park Jae Hoon , Kim Eung Soo
Published: November 2023

This study investigated the impact of MgO and rare-earth oxides (Y2O3, Yb2O3, and Dy2O3) on the structural characteristics and electrical properties of BaTiO3. Specimens sintered at 1350 °C for durations ranging from 1 to 5 h in air exhibited a single phase of BaTiO3 with a tetragonal structure. This was observed for pure BaTiO3 and specimens co-doped with MgO-Y2O3 and/or MgO-Dy2O3. However, a pseudo-cubic structure of BaTiO3 was detected for specimens doped with MgO or co-doped with MgO-Yb2O3. The unit-cell volume of the sintered specimens was found to be dependent on the type of substitution ion for the A/B site of BaTiO3 (ABO3). The dielectric constant (εr) of the sintered specimens decreased with the substitution of MgO and rare-earth oxides due to a decrease in tetragonality (c/a). The electrical resistivities of the sintered specimens were influenced not only by their microstructural characteristics but also by the secondary phases of the sintered specimens. The BaTiO3 specimens co-doped with MgO-Yb2O3 and/or doped with MgO met the EIA X7R and X8R specifications (−55 to 125~150 °C, ΔC/C = ±15% or less), respectively.
Modeling and Analysis of the Drying Process of Lithium-Ion Battery Electrodes Based on Non-Steady-State Drying Kinetics
Authors: Zhao Chunhui , Zhang Yuxin , Du Xiaozhong , Zhao Jianjun , Hu Yijian
Published: November 2023

The drying process of lithium-ion battery electrodes is one of the key processes for manufacturing electrodes with high surface homogeneity and is one of the most energy-consuming stages. The choice of the drying parameters has a significant impact on the electrode properties and the production efficiency. In response to these issues, this study establishes the non-steady-state drying kinetic equation for the electrodes, revealing the comprehensive effects of various dominant factors on the drying process. The drying rate is closely related to the electrode surface temperature, thickness, and other factors. Furthermore, this study proposes a coupled model of hot air drying field and capillary porous electrode solvent evaporation. The results showed that approximately 90% of the solvent was removed in less than half of the drying time. Then, the mechanism and control factors of electrode solvent evaporation are analyzed. During the preheating phase, the drying rate is controlled by electrode heating and temperature rise. In the constant velocity phase, it is regulated by the heat transfer from the surface airflow, while in the deceleration phase, it is affected by the mass transfer from the electrodes. Additionally, the effects of different thicknesses, temperatures, and airflow speeds on the drying process were investigated. Finally, experimental verification demonstrated the optimal parameters within the scope of the study: a temperature of 363.15 K and airflow speeds of 2.3 m/s result in a higher drying rate, as well as favorable mechanical performance.
Adolescents’ Experiences of Close Relatives Having Physical Illness, Mental Illness, Addiction/Gambling Disorders, or Death Are Associated with Poor Mental Health and Non-Suicidal Self-Injury
Authors: Tindberg Ylva , Tiikkaja Sanna
Published: November 2023

Adolescents’ lives are negatively influenced by experiences of close relatives having severe health conditions. This study aimed to investigate the associations between adolescents’ experiences of close relatives with severe health conditions (RSHCs) and poor mental health and non-suicidal self-injury (NSSI). Cross-sectional population-based data on 15–18-year-olds (n = 3483) in Sörmland, Sweden, were used to analyse the associations between RSHC experiences, such as physical illness, mental illness, addiction/gambling disorders, or death, and poor mental health and NSSI. Logistic regression models (odds ratios) were generated for having one or multiple types of RSHC experiences in different combinations, adjusting for background factors and protective factors for mental health, and stratifying by gender. Adolescents reporting multiple types of RSHC experiences had significantly increased odds ratios for poor mental health (1.74–3.07) or NSSI (1.83–3.02) compared with peers without such experiences. Adjustments for mental health protective factors attenuated the associations with poor mental health or NSSI. These associations remained significant among girls with multiple types of RSHC experiences, while boys’ vulnerabilities included having a relative who had died or had an addiction/gambling disorder. In conclusion, adolescents with RSHC experiences are at increased risk of poor mental health and NSSI. This vulnerability is most pronounced among girls with multiple RSHC experiences and especially for NSSI.
Evidence for the Involvement of the Tachykinin NK1 Receptor in Acute Inflammation of the Central Nervous System
Authors: Turner Renée J. , Nimmo Alan J.
Published: November 2023

Neuroinflammation is considered to be a significant component in a range of neuropathologies. Unfortunately, whilst its role is well recognised, the options for therapeutic intervention are limited. As such, there is a need to identify novel targets in order to increase treatment options. Given its role as both a neurotransmitter and an immune modulator, substance P (SP) and its NK1 receptor (NK1R) have been widely studied as a potential therapeutic target. There is evidence that NK1R antagonists may exert beneficial effects in a range of conditions, including traumatic brain injury and stroke. Blocking the NK1R has been shown to reduce blood–brain barrier dysfunction, reduce cerebral oedema, and reduce the levels of pro-inflammatory cytokines. These actions are associated with improved survival and functional outcomes. The NK1R has also been shown to be involved in the inflammatory reaction to CNS infection, and hence antagonists may have some benefit in reducing infection-driven inflammation. However, the NK1R may also play a role in the host immune response to infection, and so here, the potential beneficial and detrimental effects need to be carefully balanced. The purpose of this review is to provide a summary of evidence for the involvement of the NK1R in acute CNS inflammation, particularly in the context of traumatic brain injury and stroke.
Climatic and Anthropogenic Contributions to Vegetation Changes in Guangdong Province of South China
Authors: Geng Shoubao , Zhou Xia , Zhang Huamin , Yang Long , Sun Zhongyu , Yan Xiqin , Liu Meijie
Published: November 2023

How to distinguish the relative role of climate change and human activities in vegetation dynamics has attracted increasing attention. However, most of the current studies concentrate on arid and semiarid regions, while the relative contributions of climate change and human activities to vegetation changes remain unclear in warm-humid regions. Based on the normalized difference vegetation index (NDVI) and climatic variables (temperature, precipitation, radiation) during 2001–2020, this study used the Theil–Sen median trend analysis, partial correlation analysis, and residual trend analysis to analyze the spatiotemporal pattern of vegetation trends, the response of vegetation to climate variations, and the climatic and anthropogenic contributions to vegetation dynamics in the warm and humid Guangdong Province of south China. Results showed that the NDVI in most areas exhibited an increasing trend. Changes in climatic variables displayed different spatial variations which, however, were not significant in most areas. Vegetation responded diversely to climate change with temperature as the most important climatic factor for vegetation improvement in most areas, while precipitation was the dominant climatic factor in the southern edge region and radiation was the dominant climatic factor in the central and western regions. Vegetation in most areas was influenced by both climate change and human activities, but the contribution rate of human activities was commonly much higher than climate change. The findings of this study are expected to enhance our understanding of the relative climatic and anthropogenic contributions to vegetation changes in warm-humid regions and provide a scientific basis for future ecological policies and ecosystem management in highly urbanized regions.
There Is Hope in Safety Promotion! How Can Resources and Demands Impact Workers’ Safety Participation?
Authors: Margheritti Simona , Negrini Alessia , da Silva Sílvia Agostinho
Published: November 2023

Promoting workplace safety is crucial in occupational health and safety (OHS). However, existing studies have primarily concentrated on accident prevention, overlooking the role of resources in encouraging safety. This research investigates the impact of a personal resource, namely hope, on safety participation, considering its interaction with job resources and job demands using the Job Demands-Resources (JD-R) model in the context of safety. A cross-sectional study was conducted in a large company managing European shopping centers (N = 262). Of the sample, 52.3% of participants were female. Data were collected through an online questionnaire and analyzed using model 92 of Andrew F. Hayes’ Process Macro to test the hypothesized moderate serial mediation model. Our results highlighted that (1) hope directly correlates with safety participation, (2) hope and job dedication mediate the relationship between autonomy and safety participation, and (3) high job demands can undermine the beneficial effects of resources (i.e., autonomy, hope, and job dedication) on safety participation. These results suggest that workers with personal resources like hope are more likely to engage in safety practices, displaying increased dedication and focus on safety. However, excessive job demands can challenge the effectiveness of these resources in promoting safety participation. This study offers a novel perspective by integrating safety participation into the JD-R model framework.
Assessing Factors Influencing Citizens’ Behavioral Intention towards Smart City Living
Authors: Wirsbinna Aik , Grega Libor , Juenger Michael
Published: November 2023

The adoption and results achieved by “smart city” projects heavily rely on citizens’ acceptance and behavioral intention to embrace smart city living. Understanding the factors influencing citizens’ behavioral intention towards smart city living is crucial for the effective development and rollout of smart city initiatives. This research paper aims to assess the factors influencing citizens’ behavioral intention towards smart city living using quantitative research methods. Through a comprehensive literature review, an ideation structure was developed, integrating theoretical perspectives from the Technology Acceptance Model (TAM). The structure encompasses key variables such as perceived utility, convenience of use, engagement, trialability, observability, interoperability, willingness, and propensity to embrace smart city lifestyles. A quantitative methodological stance was employed to gather information from a statistically significant subset of citizens residing in urban areas in developed countries. A structured questionnaire, based on the theoretical framework, was formulated and distributed to the participants. Statistical analysis techniques, including structural equation modeling, were used for investigating connections between identified factors and citizens’ behavioral intention towards smart city living. Preliminary findings indicate that behavioral intention towards smart city living strongly depends on attitude and perceived usefulness. By addressing these factors, smart cities can foster greater citizen engagement, participation, and ultimately, the successful realization of smart city living.
Digital Séance: Fabricated Encounters with the Dead
Authors: Altaratz Doron , Morse Tal
Published: November 2023

Digital afterlife is becoming increasingly possible due to advancements in VR, deepfake, and AI technologies. The use of computational photography for mourning and commemoration has been re-integrated into practices of remembrance, farewell, continuity, and disengagement. Two case studies, the Shoah Foundation’s Dimensions in Testimony and the TV production Meeting You, are analyzed to explore these new possibilities. We show how photography’s new affordances enable interaction while maintaining its essence as a representation of reality and argue that this socio-technological transformation habituates contemporary practices of mourning and commemoration, adjusting images to serve the individual needs and interests of the bereaved and the community.
The Industrial Decision Analysis of Regional Coordinated Development Considering Information Distribution and Fairness Preference
Authors: Pan Linwei , Liao Xueyu , Li Rui , Cao Shuangping
Published: November 2023

Regional industry synergistic planning contributes to the achievement of Goal 11 of the United Nations’ Sustainable Development Goals. The reasonable layout of regional industries is an important measure for achieving sustainable development through distinguishing between different industries. Taking into account the competitive and cooperative relationships between regional cities, this study created a Stackelberg model considering information distribution and fairness preferences. It analyzed the industrial selection strategies and influencing factors under the requirements of output maximization and profit maximization between regions. The model discussions and numerical simulation results showed that regional industrial planning and adjustment should consider both internal and external behavioral factors. The impact of information distribution and fairness preferences on the selection of heterogeneous and homogeneous industries varies. Differentiated industrial selection should be conducted based on output maximization or profit maximization. Furthermore, following cities should take the initiative to integrate into the industrial development plans of nearby large cities, and leading cities should effectively layout and optimize regional productivity. Both industrial planning and choice require the establishment of regional coordination mechanisms. By enhancing the level of mutual trust and reciprocity among cities, reducing information asymmetry, and guiding fairness preferences, regional industrial synergy is promoted, and conditions are created for sustainable industrial development.