bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptive Tracking Method for Non-Cooperative Continuously Thrusting Spacecraft
Authors: Yin Juqi , Yang Zhen , Luo Yazhong
Published: September 2021

Performance of the traditional Kalman filter and its variants can seriously degrade when they are used to track a non-cooperative continuously thrusting spacecraft. To overcome this shortcoming, an adaptive tracking method for relative state estimation of a non-cooperative target is proposed based on the interacting multiple model (IMM) algorithm. First, built upon a current statistical jerk (CSJerk) model, a robust CSJerk filtering (RCSJF) algorithm is developed, which can address the issue of low estimation accuracy and instability of traditional approaches at the moments when the spacecraft starts and ends thrusting. Second, the developed RCSJF algorithm is further used to form the model set of the IMM by incorporating different maximum jerk values, based on which an adaptive tracking method is presented that can track a non-cooperative target with different maneuvering levels. Simulation results show that the proposed method can effectively track the target across all thrusts levels under the conditions considered, and the convergence performance of the proposed method is improved in comparison to the CSJerk-based extended Kalman filter, especially at the start and end time of the maneuver.
Trajectory-Tracking Controller Design of Rotorcraft Using an Adaptive Incremental-Backstepping Approach
Authors: Jung Useok , Cho Moon-Gyeang , Woo Ji-Won , Kim Chang-Joo
Published: September 2021

This paper treats a robust adaptive trajectory-tracking control design for a rotorcraft using a high-fidelity math model subject to model uncertainties. In order to control the nonlinear rotorcraft model which shows strong inter-axis coupling and high nonlinearity, incremental backstepping approach with state-dependent control effectiveness matrix is utilized. Since the incremental backstepping control suffers from performance degradation in the presence of control matrix uncertainties due to change of flight conditions, control system robustness is improved by combining the least squares parameter estimator to estimate time varying uncertainties contained in the control effectiveness matrix. Also, by selecting a suitable gain set by investigating the error dynamics, a uniform trajectory-tracking performance over operational flight envelope of the rotorcraft is ensured without resorting to the conventional gain scheduling method. To evaluate the proposed controller, comparative results between IBSC and Adaptive IBSC are provided in this paper with sequential maneuvers from the ADS-33E-PRF. The proposed method shows improved tracking performance under variations in control effective matrix in the flight simulation. Robust and stable parameter estimation is also guaranteed due to the implementation of the DF-RLS algorithm for the least squares estimator.
d2 Law and Penetration Length of Jatropha and Camelina Bio-Synthetic Paraffinic Kerosene Spray Characteristics at Take-Off, Top of Climb and Cruise
Authors: Sing Mei Sim , Anjang Ab Rahman Aslina , Abidin Mohd Shukur Zainol , Mazlan Nurul Musfirah
Published: September 2021

A comparison of d2 law and penetration length of biofuels with Jet–A through the incorporation of fuel properties and actual combustor inlet data at various flight trajectories is presented. This study aims to identify fuel properties and flight operating conditions that most influence droplet characteristics accurately. The study comprises two phases involving a simulation using GSP to predict combustor inlet data for the respective flight operating conditions and a simulation using ANSYS Fluent V18.1 to obtain combustion characteristics of biofuels and Jet–A. The biofuels chosen in this study are Jatropha Bio-synthetic Paraffinic Kerosene (JSPK) and Camelina Bio-synthetic Paraffinic Kerosene (CSPK), evaluated as pure (100%) and blend (50%) with Jet–A. Thrust specific fuel consumption (TSFC) of biofuels is improved due to lower fuel consumed by the engine. The d2 law curve shows a heat-up period that takes place at the early stage of the combustion process. The penetration length of the fuels is shorter at take-off. Combusting biofuels reduce combustion temperature and the penetration length of the droplet.
A Hybrid Real/Ideal Gas Mixture Computational Framework to Capture Wave Propagation in Liquid Rocket Combustion Chamber Conditions
Authors: D’Alessandro Simone , Pizzarelli Marco , Nasuti Francesco
Published: September 2021

The present work focuses on the development of new mathematical and numerical tools to deal with wave propagation problems in a realistic liquid rocket chamber environment. A simplified real fluid equation of state is here derived, starting from the literature. An approximate Riemann solver is then specifically derived for the selected conservation laws and primitive variables. Both the new equation of state and the new Riemann solver are embedded into an in-house one-dimensional CFD solver. The verification and validation of the new code against wave propagation problems are then performed, showing good behavior. Although such problems might be of interest for different applications, the present study is specifically oriented to the low order modeling of high-frequency combustion instability in liquid-propellant rocket engines.
Estimation of Genetic Parameters for Feeding Pattern Traits and Its Relationship to Feed Efficiency and Production Traits in Duroc Pigs
Authors: Santiago Kier G. , Kim Sung-Hoon , Lopez Bryan Irvine , Lee Dong-Hui , Cho Young-Gyu , Song Yu-Na , Seo Kang-Seok
Published: September 2021

This study was conducted to estimate the genetic parameters of different feeding pattern traits, including average daily feed intake (ADFI), average occupation time per day (AOTD), average occupation time per visit (AOTV), average daily feeding rate (ADFR), average feeding rate per feeding visit (AFRV), average feed intake per feeding visit (AFIV), and average number of visits per day (ANVD), and their genetic relationship to production traits, such as on-test average daily gain (ADG), backfat thickness (BFT), loin muscle area (LMA), lean percentage (LP), and feed efficiency traits, such as feed conversion ratio (FCR) and five measures of residual feed intake (RFI1 to RFI5), in Duroc pigs (DD). The non-heritable common spatial pen effect was also estimated in all studied traits. The feeding pattern traits used in this study were derived from filtered feeding visits of 602 DD pigs. Using three animal models and the REML method, the genetic parameters revealed low to moderate heritability for ADFI (0.19 to 0.32) and AFIV (0.18 to 0.33), moderate heritability for ANVD (0.28 to 0.35) and AOTV (0.21 to 0.31), and high heritability for AOTD (0.73), ADFR (0.62 to 0.64), and AFRV (0.59 to 0.63). The addition of a common spatial pen effect in models 2 and 3 had a substantial impact, ranging from 8% to 23%, on the total variability of most feeding pattern traits, with the exception of AOTD, which only had a percentage variance of 0.30% due to the pen effect. The genetic and phenotypic correlation revealed that ADFI had consistent moderate to high genetic and phenotypic correlation with production and feed efficiency (FE) traits. However, selection against ADFI would negatively affect on-test ADG. Interestingly, the AOTD had no genetic correlation with ADG (0.04), low to moderate positive genetic correlation with FCR (0.27) and all RFI measures (0.24 to 0.33), and moderate negative correlation with LP (−0.39), indicating that selection for DD pigs with lower AOTD would not influence on-test ADG but may increase LP and improve feed efficiency by lowering FCR and all RFI measures. However, the corresponding phenotypic correlation of AOTD with production and feed efficiency traits was mostly weak, which can be attributed to the low residual or environmental correlation between these correlated traits. At the genetic level, the feeding pattern traits showed potential in improving feed efficiency and production traits. However, further studies are needed to evaluate their impact at phenotypic level.
Half Diallel Analysis for Biochemical and Morphological Traits in Cultivated Eggplants (Solanum melongena L.)
Authors: Datta Debi Rani , Rafii Mohd Y. , Misran Azizah , Jusoh Mashitah , Yusuff Oladosu , Haque Md. Azadul , Jatto Muhammed Itopa
Published: September 2021

Eleven morphologically diverse cultivated eggplant accessions were used for hybridization following half diallel mating design to obtain 55 hybrids. Evaluation of hybrids along with the parents was conducted over two locations followed by randomised complete block design with three replications to study gene action and combining ability of 15 morphological and biochemical traits. The analysis of variance indicated highly significant differences among the environments and interaction of genotype and environment, except for fruit length to width ratio. Additive gene effects were significant for the inheritance of these traits and expression of these additive genes were greatly affected by environments. The general combining ability (GCA) was greater than their respective specific combining ability (SCA) for all traits except for fruit yield per plant. High values of GCA and SCA effects for characters of interest were dispersed among different genotypes. From this study it was observed that the best parental line was BT15 based on days to first flowering, total number of fruits per plant, total soluble solids and total phenol content. Besides, the parent BM5 showed good general combining ability effects for fruit yield per plant, fruit length and fruit length to width ratio and the parent BB1 performed good general combining ability for fruit diameter, fruit girth and fruit weight. Besides, other parents showed the best performance for only one trait. On the other hand, the hybrid BT6 × BT15 was reported bearing early flowering with high total phenol content and the hybrid BM9 × BB26 has high fruit yield with high soluble solids. Besides, the hybrid BM9 × BB1 has a high fruit diameter and fruit weight. All other hybrids except for these three (BT6 × BT15, BM9 × BB26 and BM9 × BB1) were shown the best performance for only one trait. Hence, based on the desired trait, the hybrid can be selected for future use after large scale evaluation.
Environmental and Animal-Related Parameters and the Emissions of Ammonia and Methane from an Open-Sided Free-Stall Barn in Hot Mediterranean Climate: A Preliminary Study
Authors: D'Urso Provvidenza Rita , Arcidiacono Claudia , Cascone Giovanni
Published: September 2021

Increased knowledge on the factors that affect emissions from open-sided dairy buildings may lead to an improvement of the mitigation strategies. In this study, ammonia (NH3) and methane (CH4) emissions were assessed in an open dairy barn in a hot Mediterranean climate at different managements of the cooling system, as well as the influence of environmental and animal-related parameters on daily emissions. Measurements of gas concentrations and micro-climatic parameters were carried out in a cubicle free-stall dairy barn located in the province of Ragusa (Italy) in two weeks of 2016 characterised by similar climatic conditions in the warm period. Emissions of NH3 and CH4 were estimated through the application of the carbon-dioxide (CO2) mass balance method. Data collected were organised in specific datasets to carry out different statistical analyses on gas emissions depending on selected parameters for the two weeks with a different management of the cooling system. The results showed higher NH3 emissions and lower CH4 emissions in W1 than those in W2. The variability in gas emissions was related to the effect of temperature humidity index (THI) (p < 0.001) and cow behaviour (p < 0.01). The highest emissions were recorded during the cleaning procedures for both NH3 (p < 0.001) and CH4 (p < 0.001), whereas the lowest emissions were recorded during the central hours of the day.
Tillage and Liquid Dairy Manure Effects on Overland Flow Nitrogen and Phosphorus Loss Potential in an Upper Midwest Corn Silage-Winter Triticale Cropping System
Authors: Sherman Jessica F. , Young Eric O. , Cavadini Jason
Published: September 2021

Dairy manure is an important crop nutrient source in Wisconsin and other parts of the upper Midwest but can contribute to nitrogen (N) and phosphorus (P) losses in overland flow/surface runoff. Winter cereal grain cover crops can help reduce erosion and nutrient transport in corn systems. However, few studies have compared tillage impacts on nutrient loss in live cover crop systems. The objective of this study was to evaluate vertical (VT) and chisel tillage (CT) effects on overland flow nutrient and sediment loss potential after spring-applied liquid manure. A surface application treatment (i.e., broadcast) and a no manure control were also included for comparison. After corn (Zea mays L.) planting into a live triticale (Triticale hexaploide L.) cover crop, four artificial rainfall-overland flow events were generated (42 mm h−1 for 30 min) on replicated field-scale plots in central Wisconsin. Mean total P, total N, and suspended solids loads were consistently lower for VT at 2 days post-manure application (with 97 to 99% lower losses than broadcast, respectively). Dissolved reactive P and ammonium-N concentrations for both CT and VT were significantly lower three weeks after manure application compared to broadcast. Results suggest that VT reduced soil/residue disturbance while incorporating manure sufficiently to reduce sediment, N, and P transport potential under simulated high overland flow conditions.
Socioeconomic Impact of Climate Change in Rural Areas of Greece Using a Multicriteria Decision-Making Model
Authors: Georgilas Ioannis , Moulogianni Christina , Bournaris Thomas , Vlontzos George , Manos Basil
Published: September 2021

Agriculture is the main and, in some cases, the only, source of income and employment in rural areas. The change in the conditions under which agriculture is practiced has various effects on the agricultural economy but also on the social structure of rural areas. Climate change has multiple effects on agricultural production, necessitating the reorganization of agricultural production in some cases. These effects of climate change will also impact the economic and social aspects of farms in rural areas. This paper attempts to identify these effects by measuring the socioeconomic impacts of climate change in the region of Central Macedonia in Greece. For this reason, a multicriteria model was developed to simulate these impacts by estimating a set of seven social and economic indicators. The model was implemented to the average farm which was estimated from the main cultivations of the region. A scenario analysis was also used in combination with the multicriteria model. The multicriteria model suggests modifications are needed in the average farm crop plan of the region as a result of the climate change impact. The scenarios results show that climate change will negatively affect all the social and economic indicators and will continue to affect them over the years. These results can be used by policymakers to understand the economic and social impacts of climate change in the region to plan their future policies.
An Approach to Drought Vulnerability Assessment Focused on Groundwater Wells in Upland Cultivation Areas of South Korea
Authors: Shin Hyungjin , Lee Gyumin , Lee Jaenam , Lee Jaeyoung , Park Minji , Park Changi
Published: September 2021

This study aimed to present an approach that identifies priority management areas to drought focused on field crops and groundwater wells using the MCDM method. Groundwater wells are the primary source of water during drought for field crops. Nevertheless, the systematic management of groundwater wells has not been achieved. Thus, this paper intends to establish a plan that can manage groundwater wells through a vulnerability assessment. This study used TOPSIS, a widely applied multi-criterion decision-making algorithm, to evaluate 158 cities and counties in Korea. This study chose the assessment factors by focusing on drought and classifying the positive and negative elements of the wells. Precipitation, groundwater level, and pumping capacity were considered to have positive effects, while cultivated area, the number of consecutive days without rain, and the proportion of private groundwater wells were considered as negative factors. As a result, the pumping capacity per cultivation area is the major factor affecting management priorities and groundwater well vulnerability. This study presents an approach to assess the drought vulnerability of field crops focused on groundwater wells and select a priority management area, which facilitates efficient well management and reduces damage to crops caused by local droughts.
Biological Activities of Methanolic Extract of Aegle marmelos against HN Protein of Newcastle Disease Virus
Authors: Andleeb Rahat , Ijaz Muhammad Umar , Rafique Azhar , Ashraf Asma , Bano Naheed , Zafar Nimrah , Tasleem Faisal , Marc (Vlaic) Romina Alina , Pop Oana Lelia , Ahmedah Hanadi Talal
Published: September 2021

The current study explores the methanolic extracts of the leaves and fruit of Aegle marmelos (Bael) for their total phenolic content (TPC), total flavonoids content (TFC), antioxidants, and antibiofilms, as well as its in ovo antiviral potential against Newcastle disease virus (NDV). The drug-likeliness thereof and the potential identification of an interaction—their molecular docking of ligands with target proteins by GOLD—was determined in silico using the Swiss ADME software. The total flavonoids content (TFC) was 135.17 ± 2.02 and 111.2 ± 3.67 mg QE/g, while the total phenolics content (TPC) was 185.02 ± 2.15 and 171.13 ± 6.73 mg GAE/g, in the fruit and leaves extracts, respectively. In a DPPH assay, the IC50 value for the methanolic extracts of leaves and fruit was 63.52 ± 1.48 and 52.06 ± 1.62. μg/mL d.w. The fruit extract of A. marmelos showed significantly higher reducing power (i.e., 59.32 ± 0.05 µmol/g d.w) than the leaves extract (p < 0.05). The biofilm-inhibition activity of the fruit extract of A. marmelos was 65.78 ± 0.65 µg/mL. Both parts of the plant showed potent antiviral potential at higher concentrations. A study in silico, using the molecular docking of three compounds, showed good interaction with the HN protein, with considerable binding affinities and fulfilled docking parameters. This work shows that Aegle marmelos and its phytoconstituents can be used as a potential remedy for NDV.
Effect of Pre-Milking Teat Foam Disinfection on the Prevention of New Mastitis Rates in Early Lactation
Authors: Fitzpatrick Sarah Rose , Garvey Mary , Flynn Jim , O’Brien Bernadette , Gleeson David
Published: September 2021

The objective of this study was to determine the benefit of pre-milking teat foam disinfection on the prevention of new infections by contagious and environmental bacteria in two spring calving herds managed outdoors (Herd 1 [H1]; 331 cows and Herd 2 [H2]; 142 cows). Four pre-milking teat preparation treatments were applied post calving; with each herd receiving two treatments; using a split udder design (for approx. 15 weeks). These treatments included; (1) ‘water wash, foam application and dry wipe (WFD) in H1′; (2) ‘water wash and dry wipe (WD)’ in H1; (3) ‘foam application and dry wipe (FD)’ in H2; (4) ‘no teat cleaning preparation (NP)’ in H2. Individual quarter foremilk samples were collected on four occasions and all clinical and sub-clinical cases were recorded. The mean SCC of quarter foremilk samples was 134 × 103 cells/mL and 127 × 103 cells/mL for WD and WFD, respectively, and 109 × 103 cells/mL and 89 × 103 cells/mL for NP and FD, respectively (p > 0.05). Lower bacterial counts were observed on teat skin that received a foaming treatment. Pre-milking teat disinfection using a foaming product may be of little benefit, in early lactation, for a pasture-based dairy herd.
Influence of Speed, Ground Surface and Shoeing Condition on Hoof Breakover Duration in Galloping Thoroughbred Racehorses
Authors: Horan Kate , Coburn James , Kourdache Kieran , Day Peter , Harborne Dan , Brinkley Liam , Carnall Henry , Hammond Lucy , Peterson Mick , Millard Sean , Pfau Thilo
Published: September 2021

Understanding the effect of horseshoe–surface combinations on hoof kinematics at gallop is relevant for optimising performance and minimising injury in racehorse–jockey dyads. This intervention study assessed hoof breakover duration in Thoroughbred ex-racehorses from the British Racing School galloping on turf and artificial tracks in four shoeing conditions: aluminium, barefoot, aluminium–rubber composite (GluShu) and steel. Shoe–surface combinations were tested in a randomized order and horse–jockey pairings (n = 14) remained constant. High-speed video cameras (Sony DSC-RX100M5) filmed the hoof-ground interactions at 1000 frames per second. The time taken for a hoof marker wand fixed to the lateral hoof wall to rotate through an angle of 90 degrees during 384 breakover events was quantified using Tracker software. Data were collected for leading and non-leading forelimbs and hindlimbs, at gallop speeds ranging from 23–56 km h−1. Linear mixed-models assessed whether speed, surface, shoeing condition and any interaction between these parameters (fixed factors) significantly affected breakover duration. Day and horse–jockey pair were included as random factors and speed was included as a covariate. The significance threshold was set at p < 0.05. For all limbs, breakover times decreased as gallop speed increased (p < 0.0005), although a greater relative reduction in breakover duration for hindlimbs was apparent beyond approximately 45 km h−1. Breakover duration was longer on turf compared to the artificial surface (p ≤ 0.04). In the non-leading hindlimb only, breakover duration was affected by shoeing condition (p = 0.025) and an interaction between shoeing condition and speed (p = 0.023). This work demonstrates that speed, ground surface and shoeing condition are important factors influencing the galloping gait of the Thoroughbred racehorse.
Effect of a Mineral–Microbial Deodorizing Preparation on the Functions of Internal Organs and the Immune System in Commercial Poultry
Authors: Kowalczyk Joanna , Tykałowski Bartłomiej , Śmiałek Marcin , Stenzel Tomasz , Dziewulska Daria , Koncicki Andrzej
Published: September 2021

Animal production is identified as one of the main sources of high concentrations of odours, which are related to air pollution, health problems of living organisms and indirect negative impact on production results. One common method for reducing emissions of ammonia is using preparations containing probiotics and hygroscopic or disinfecting compounds. This study was undertaken in order to determine the impact of innovative mineral–microbial deodorizing preparation, which reduces odorous gases, applying to the litter once a week in poultry houses on the physiological status of breeder chickens, broiler chickens and turkeys. Samples were collected after slaughter and analyzed using ELISA tests, flow cytometry and biochemical methods. Biochemical markers of the liver and kidney profile (ALT, AST, LDH, ALP, CK, TP, CALC, PHOS) and the titers of specific antibodies against AEV, aMPV, AAvV-1, IBDV, HEV, BA were analyzed in serum samples. The percentage contribution of T and B lymphocyte subpopulations was determined in the samples of tracheal mucosa, blood, and spleen. No significant differences were found between the control and experimental group with regard to all the analyzed parameters, with some exceptions for biochemistry. The results of our study indicated that mineral–microbial deodorizing preparation did not affect the physiological status of birds.
ISGylation Inhibits an LPS-Induced Inflammatory Response via the TLR4/NF-κB Signaling Pathway in Goat Endometrial Epithelial Cells
Authors: Xiao Jinbang , Li Shanshan , Zhang Ruixue , Wang Zongjie , Zhang Xinyan , Wang Aihua , Jin Yaping , Lin Pengfei
Published: September 2021

Endometritis is a common and important reproductive disease of domestic animals. The principal factors responsible for the disease are infection with Gram-negative bacteria, the release of Lipopolysaccharides (LPS) and activation of the TLR4/NF-κB signaling pathway. However, we do not fully understand the interaction between endometrial immunity and bacterial infection in the disease etiology. The ubiquitin-like protein ISG15 can regulate the TLR4/NF-κB signaling pathway via the ISGylation modification system, modulating the inflammatory response. In the present study, we found that ISG15 protein was expressed mainly in the cytoplasm of goat endometrial epithelial cells (gEECs) and that the expression of key genes and proteins of ISGylation increased in LPS-induced gEECs. Overexpression and silencing of the ISG15 gene demonstrated that ISGylation inhibited an LPS-induced inflammatory response via the TLR4/NF-κB signaling pathway in gEECs. Here, we provide the experimental basis for further exploration of the role of the ISGylation modification system in the inflammatory response of endometrium and a potential method for the treatment of endometritis.
Neurohormonal Profiles of Assistance Dogs Compared to Pet Dogs: What Is the Impact of Different Lifestyles?
Authors: Mengoli Manuel , Oliva Jessica L. , Mendonça Tiago , Chabaud Camille , Arroub Sana , Lafont-Lecuelle Céline , Cozzi Alessandro , Pageat Patrick , Bienboire-Frosini Cécile
Published: September 2021

Assistance dogs must manage stress efficiently because they are involved in challenging tasks. Their welfare is currently a fundamental issue. This preliminary study aimed to compare assistance dogs (AD; n = 22) with pet dogs (PD; n = 24), using blood neuromodulator indicators to help find biomarkers that can improve the AD breeding, selection, training, and welfare monitoring. Both populations originated from different breeds, are of different ages, and had different lifestyles. Basal peripheral concentrations of prolactin (PRL), serotonin (5-HT), free (fOT) and total (tOT) oxytocin were measured by immunoassays. Multiple linear regressions were performed to assess the effect of activity, age, sex, and their interactions on these parameters. Correlations between neurohormonal levels were analyzed. No interactions were significant. fOT and tOT concentrations were significantly influenced by age (p < 0.0001 and p = 0.0002, respectively) and dogs’ activity (p = 0.0006 and p = 0.0277, respectively). A tendency was observed for age effect on PRL (p = 0.0625) and 5-HT (p = 0.0548), as well as for sex effect on tOT (p = 0.0588). PRL concentrations were heterogenous among AD. fOT and tOT were significantly but weakly correlated (Pearson’s r = 0.34; p = 0.04). Blood prolactin, serotonin, and oxytocin may represent biomarkers to assess workload and chronic stress-related responses in ADs and eventually improve their selection and training.
Effect of a Low-Methane Diet on Performance and Microbiome in Lactating Dairy Cows Accounting for Individual Pre-Trial Methane Emissions
Authors: Chagas Juana C. , Ramin Mohammad , Exposito Ruth Gomez , Smidt Hauke , Krizsan Sophie J.
Published: September 2021

This study examined the effects of partly replacing grass silage (GS) with maize silage (MS), with or without rapeseed oil (RSO) supplementation, on methane (CH4) emissions, production performance, and rumen microbiome in the diets of lactating dairy cows. The effect of individual pre-trial CH4-emitting characteristics on dietary emissions mitigation was also examined. Twenty Nordic Red cows at 71 ± 37.2 (mean ± SD) days in milk were assigned to a replicated 4 × 4 Latin square design with four dietary treatments (GS, GS supplemented with RSO, GS plus MS, GS plus MS supplemented with RSO) applied in a 2 × 2 factorial arrangement. Partial replacement of GS with MS decreased the intake of dry matter (DM) and nutrients, milk production, yield of milk components, and general nutrient digestibility. Supplementation with RSO decreased the intake of DM and nutrients, energy-corrected milk yield, composition and yield of milk fat and protein, and general digestibility of nutrients, except for crude protein. Individual cow pre-trial measurements of CH4-emitting characteristics had a significant influence on gas emissions but did not alter the magnitude of CH4 emissions. Dietary RSO decreased daily CH4, yield, and intensity. It also increased the relative abundance of rumen Methanosphaera and Succinivibrionaceae and decreased that of Bifidobacteriaceae. There were no effects of dietary MS on CH4 emissions in this study, but supplementation with 41 g RSO/kg of DM reduced daily CH4 emissions from lactating dairy cows by 22.5%.
Toward Precise Nutrient Value of Feed in Growing Pigs: Effect of Meal Size, Frequency and Dietary Fibre on Nutrient Utilisation
Authors: Chassé Élisabeth , Guay Frédéric , Bach Knudsen Knud Erik , Zijlstra Ruurd T. , Létourneau-Montminy Marie-Pierre
Published: September 2021

Nutritional values of ingredients have been and still are the subject of many studies to reduce security margins of nutrients when formulating diets to reduce feed cost. In most studies, pigs are fed a limited amount of feed in a limited number of meals that do not represent how pigs are fed in commercial farm conditions. With free access to feed, pigs follow their intrinsic feeding behaviour. Feed intake is regulated by satiety and satiation signals. Reducing the feed intake level or feeding frequency can affect digestibility and transit time and induce metabolic changes. To reduce feed costs, alternative ingredients that are frequently rich in dietary fibre are added to diets. Fibre acts on the digestion process and transit time by decreasing energy density and causing viscosity. Various analyses of fibre can be realised, and the measured fibre fraction can vary. Exogenous enzymes can be added to counteract the effect of fibre, but digestive tract conditions, influenced by meal size and frequency, can affect the efficiency of supplemented enzymes. In conclusion, the frequency and size of the meals can affect the digestibility of nutrients by modulating gastrointestinal tract conditions (pH and transit time), metabolites (glucose and short-chain fatty acids) and hormones (glucagon-like peptide 1 and peptide tyrosine tyrosine).
Towards Efficient Early Warning: Pathobiology of African Swine Fever Virus “Belgium 2018/1” in Domestic Pigs of Different Age Classes
Authors: Pikalo Jutta , Schoder Marie-Eve , Sehl-Ewert Julia , Breithaupt Angele , Cay Ann Brigitte , Lhoëst Coline , van Campe Willem , Mostin Laurent , Deutschmann Paul , Roszyk Hanna , Beer Martin , Blome Sandra , Tignon Marylène
Published: September 2021

African swine fever (ASF) is one of the most important and devastating viral diseases in wild boar and domestic pigs worldwide. In the absence of vaccines or treatment options, early clinical detection is crucial and requires a sound knowledge of disease characteristics. To provide practitioners and state veterinarians with detailed information, the objective of the present study was to characterize the ASF virus (ASFV) isolate “Belgium 2018/1” in subadult and weaning domestic pigs. To this end, two animal trials were performed. Trial A included eight subadult domestic pigs and trial B five weaner pigs. In general, clinical signs and pathological lesions were in line with previous studies utilizing highly virulent ASF genotype II viruses. However, in trial A, four subadult domestic pigs survived and recovered, pointing to an age-dependent outcome. The long-term fate of these survivors remains under discussion and would need further investigation.
Osmoregulatory Plasticity of Juvenile Greater Amberjack (Seriola dumerili) to Environmental Salinity
Authors: Barany Andre , Gilannejad Neda , Alameda-López María , Rodríguez-Velásquez Liliana , Astola Antonio , Martínez-Rodríguez Gonzalo , Roo Javier , Muñoz Jose Luis , Mancera Juan Miguel
Published: September 2021

Osmotic costs in teleosts are highly variable, reaching up to 50% of energy expenditure in some. In several species, environmental salinities close to the isosmotic point (~15 psu) minimize energy demand for osmoregulation while enhancing growth. The present study aimed to characterize the physiological status related to osmoregulation in early juveniles of the greater amberjack, Seriola dumerili, acclimated to three salinities (15, 22, and 36 psu). Our results indicate that plasma metabolic substrates were enhanced at the lower salinities, whereas hepatic carbohydrate and energetic lipid substrates decreased. Moreover, osmoregulatory parameters, such as osmolality, muscle water content, gill and intestine Na+-K+-ATPase activities, suggested a great osmoregulatory capacity in this species. Remarkably, electrophysiological parameters, such as short-circuit current (Isc) and transepithelial electric resistance (TER), were enhanced significantly at the posterior intestine. Concomitantly, Isc and TER anterior-to-posterior intestine differences were intensified with increasing environmental salinity. Furthermore, the expression of several adeno-hypophyseal genes was assessed. Expression of prl showed an inverse linear relationship with increasing environmental salinity, while gh mRNA enhanced significantly in the 22 psu-acclimated groups. Overall, these results could explain the better growth observed in S. dumerili juveniles kept at salinities close to isosmotic rather than in seawater.
Soybean Oil Replacement by Poultry Fat in Broiler Diets: Performance, Nutrient Digestibility, Plasma Lipid Profile and Muscle Fatty Acids Content
Authors: Saleh Ahmed A. , Alharthi Abdulrahman S. , Alhotan Rashed A. , Atta Mustafa Shukry , Abdel-Moneim Abdel-Moneim Eid
Published: September 2021

Continuous genetic improvements of commercial broiler strains has led to the necessity of using fats in their rations to fulfill a large portion of the energetic requirements. Several fat sources have been introduced in poultry nutrition, such as rendering poultry fat (PF) an available and cheap lipid source compared to conventional sources such as soybean oil (SO). The present study investigated the effect of partial or full replacement of SO by PF on performance, nutrient digestibility, blood lipids, and fatty acids (FAs) content of pectoral muscle. Four hundred and eighty one-day-old male Ross-308 chicks were distributed into four experimental groups (12 replicates each): the first group (control) was fed a diet formulated with soybean oil as a fat source while the second to fourth groups (PF25, PF50, and PF100) were fed diets formulated with 25, 50 and 100% of PF as a fat source instead of SO. Results revealed no synergistic effect between SO and PF in any of the studied parameters. Replacing SO by PF did not alter birds’ growth, carcass characteristics, and plasma indices of birds. Abdominal fat% was increased (p < 0.01) in PF50 and PF100. Dry matter digestibility was improved (p < 0.05) in PF50 and PF100, while crude fat and protein digestibility was not affected. Contents of palmitic and docosahexaenoic acids in the pectoral muscle of PF50 and PF100 were reduced (p < 0.01) while concentrations of oleic and linolenic acids, total unsaturated FAs, and polyunsaturated FAs/Saturated FAs ratio were elevated (p < 0.05) in the same groups. Liver thiobarbituric acid reactive substances (TBARS) and muscle vitamin E contents were not altered. The dietary addition of PF greatly improved economic parameters. In conclusion, PF can be used as a lipid source in broiler diets to produce inexpensive meat while maintaining its growth performance.
Increased Inflammatory Markers Detected in Nasal Lavage Correlate with Paranasal Sinus Abnormalities at MRI in Adolescent Patients with Cystic Fibrosis
Authors: Chung Jaehi , Wünnemann Felix , Salomon Johanna , Boutin Sébastien , Frey Dario L. , Albrecht Tobias , Joachim Cornelia , Eichinger Monika , Mall Marcus A. , Wielpütz Mark O. , Sommerburg Olaf
Published: September 2021

Chronic rhinosinusitis (CRS) is a characteristic feature of cystic fibrosis (CF) multiorgan disease and develops early in the life of patients with CF. The study aimed to correlate the inflammatory markers and the presence of structural abnormalities detected by MRI in the paranasal sinuses of patients with CF. Methods: Nasal lavage and MRI of the paranasal sinuses was performed in a cohort of 30 CF patients (median age 14 y; range 7–20 y). Morphological abnormalities characteristic of CF were evaluated with a dedicated CRS MRI scoring system and correlated with different inflammation parameters measured in nasal lavage. Inflammation of the paranasal sinuses was positively associated with structural abnormalities in MRI. The concentration of the pro-inflammatory markers neutrophil elastase (NE) and the neutrophil elastase/alpha1-antitrypsin (NE/A1AT) complex correlated significantly with CRS-MRI sum score (p < 0.05, r = 0.416 and p < 0.05, r = 0.366, respectively). S. aureus infection was associated with the increased pro-inflammatory cytokine activity of IL-6 and IL-8, and increased levels of NE/A1AT complex in our patients (p < 0.05, respectively). CRS-MRI sum score and individual sinus MRI scores were positively associated with inflammatory activity as a sign of CRS pathology present in CF.
SVCT2 Overexpression and Ascorbic Acid Uptake Increase Cortical Neuron Differentiation, Which Is Dependent on Vitamin C Recycling between Neurons and Astrocytes
Authors: Salazar Katterine , Espinoza Francisca , Cerda-Gallardo Gustavo , Ferrada Luciano , Magdalena Rocío , Ramírez Eder , Ulloa Viviana , Saldivia Natalia , Troncoso Ninoschka , Oviedo María José , Barahona María José , Martínez Fernando , Nualart Francisco
Published: September 2021

During brain development, sodium–vitamin C transporter (SVCT2) has been detected primarily in radial glial cells in situ, with low-to-absent expression in cerebral cortex neuroblasts. However, strong SVCT2 expression is observed during the first postnatal days, resulting in increased intracellular concentration of vitamin C. Hippocampal neurons isolated from SVCT2 knockout mice showed shorter neurites and low clustering of glutamate receptors. Other studies have shown that vitamin C-deprived guinea pigs have reduced spatial memory, suggesting that ascorbic acid (AA) and SVCT2 have important roles in postnatal neuronal differentiation and neurite formation. In this study, SVCT2 lentiviral overexpression induced branching and increased synaptic proteins expression in primary cultures of cortical neurons. Analysis in neuroblastoma 2a (Neuro2a) and human subventricular tumor C3 (HSVT-C3) cells showed similar branching results. SVCT2 was mainly observed in the cell membrane and endoplasmic reticulum; however, it was not detected in the mitochondria. Cellular branching in neuronal cells and in a previously standardized neurosphere assay is dependent on the recycling of vitamin C or reduction in dehydroascorbic acid (DHA, produced by neurons) by glial cells. The effect of WZB117, a selective glucose/DHA transporter 1 (GLUT1) inhibitor expressed in glial cells, was also studied. By inhibiting GLUT1 glial cells, a loss of branching is observed in vitro, which is reproduced in the cerebral cortex in situ. We concluded that vitamin C recycling between neurons and astrocyte-like cells is fundamental to maintain neuronal differentiation in vitro and in vivo. The recycling activity begins at the cerebral postnatal cortex when neurons increase SVCT2 expression and concomitantly, GLUT1 is expressed in glial cells.
Emerging Green Techniques for the Extraction of Antioxidants from Agri-Food By-Products as Promising Ingredients for the Food Industry
Authors: Carpentieri Serena , Soltanipour Farid , Ferrari Giovanna , Pataro Gianpiero , Donsì Francesco
Published: September 2021

Nowadays, the food industry is heavily involved in searching for green sources of valuable compounds, to be employed as potential food ingredients, to cater to the evolving consumers’ requirements for health-beneficial food ingredients. In this frame, agri-food by-products represent a low-cost source of natural bioactive compounds, including antioxidants. However, to effectively recover these intracellular compounds, it is necessary to reduce the mass transfer resistances represented by the cellular envelope, within which they are localized, to enhance their extractability. To this purpose, emerging extraction technologies, have been proposed, including Supercritical Fluid Extraction, Microwave-Assisted Extraction, Ultrasound-Assisted Extraction, High-Pressure Homogenization, Pulsed Electric Fields, High Voltage Electrical Discharges. These technologies demonstrated to be a sustainable alternative to conventional extraction, showing the potential to increase the extraction yield, decrease the extraction time and solvent consumption. Additionally, in green extraction processes, also the contribution of solvent selection, as well as environmental and economic aspects, represent a key factor. Therefore, this review focused on critically analyzing the main findings on the synergistic effect of low environmental impact technologies and green solvents towards the green extraction of antioxidants from food by-products, by discussing the main associated advantages and drawbacks, and the criteria of selection for process sustainability.
The Effect of Oxidative Stress on the Chicken Ovary: Involvement of Microbiota and Melatonin Interventions
Authors: Wang Jianping , Jia Ru , Gong Haojie , Celi Pietro , Zhuo Yong , Ding Xuemei , Bai Shiping , Zeng Qiufeng , Yin Huadong , Xu Shengyu , Liu Jingbo , Mao Xiangbing , Zhang Keying
Published: September 2021

The poultry ovary is used as a classic model to study ovarian biology and ovarian cancer. Stress factors induced oxidative stress to cause follicle atresia, which may be a fundamental reason for the reduction in fertility in older laying hens or in aging women. In the present study, we set out to characterize the relationships between oxidative stress and ovarian function. Layers (62 weeks of age; BW = 1.42 ± 0.12 kg) were injected with tert-butyl hydroperoxide (tBHP) at 0 (CON) and 800 μmol/kg BW (oxidative stress group, OS) for 24 days and the role of melatonin (Mel) on tBHP-induced ovary oxidative stress was assessed through ovary culture in vitro. The OS (800 μmol/kg BW tert-butyl hydroperoxide) treatment decreased the reproduction performance and ovarian follicle numbers. OS decreased the expression of SIRT1 and increased the P53 and FoxO1 expression of the ovary. A decreased Firmicutes to Bacteroidetes ratio, enriched Marinifilaceae (family), Odoribacter (genus) and Bacteroides_plebeius (species) were observed in the cecum of the OS group. Using Mel in vitro enhanced the follicle numbers and decreased the ovary cell apoptosis induced by tBHP. In addition, it increased the expression of SIRT1 and decreased the P53 and FoxO1 expression. These findings indicated that oxidative stress could decrease the laying performance, ovarian function and influence gut microbiota and body metabolites in the layer model, while the melatonin exerts an amelioration the ovary oxidative stress through SIRT1-P53/FoxO1 pathway.
Stereolithographic Additive Manufacturing of Zirconia Electrodes with Dendritic Patterns for Aluminum Smelting
Authors: Takahashi Masaya , Kirihara Soshu
Published: September 2021

Zirconia electrodes with dendritic patterns were fabricated by stereolithographic additive manufacturing (STL-AM). A solid electrolyte of yttria-stabilized zirconia (YSZ) was selected for oxygen separation in the molten salt electrolysis of aluminum smelting without carbon dioxide excretion. Thereafter, 4, 6, 8 and 12-coordinated dendritic structures composed of cylindrical lattices were designed as computer graphics. The specific surface area of each structure was maximized by changing the aspect ratio. The spatial profile and surface pressure of the hot liquid propagation in the dendrite patterns were systematically visualized by computational fluid dynamics (CFD). During the fabrication process, a photosensitive resin containing zirconia particles was spread on a substrate, and an ultraviolet (UV) laser beam was scanned to create a two-dimensional (2D) cross-section. Through layer laminations, three-dimensional (3D) objects with dendritic structures were successfully fabricated. The ceramics were obtained through dewaxing and sintering.
A Real-Time BLE/PDR Integrated System by Using an Improved Robust Filter for Indoor Position
Authors: Xu Shenglei , Wang Yunjia , Sun Meng , Si Minghao , Cao Hongji
Published: September 2021

Indoor position technologies have attracted the attention of many researchers. To provide a real-time indoor position system with high precision and stability is necessary under many circumstances. In a real-time position scenario, gross errors of the Bluetooth low energy (BLE) fingerprint method are more easily occurring and the heading angle of the pedestrian will drift without acceleration and magnetic field compensation. A real-time BLE/pedestrian dead-reckoning (PDR) integrated system by using an improved robust filter has been proposed. In the PDR method, the improved Mahony complementary filter based on the pedestrian motion states is adopted to estimate the heading angle reducing the drift error. Then, an improved robust filter is utilized to detect and restrain the gross error of the BLE fingerprint method. The robust filter detected the gross error at different granularity by constructing a robust vector changing the observation covariance matrix of the extended Kalman filter (EKF) adaptively when the application is running. Several experiments are conducted in the true position scenario. The mean position accuracy obtained by the proposed method in the experiment is 0.844 m and RMSE is 0.74 m. Compared with the classic EKF, these two values are increased by 38% and 18%, respectively. The results show that the improved filter can avoid the gross error in the BLE method and provide high precision and scalability in indoor position service.
Enhancement of Text Analysis Using Context-Aware Normalization of Social Media Informal Text
Authors: Khan Jebran , Lee Sungchang
Published: September 2021

We proposed an application and data variations-independent, generic social media Textual Variations Handler (TVH) to deal with a wide range of noise in textual data generated in various social media (SM) applications for enhanced text analysis. The aim is to build an effective hybrid normalization technique that ensures the use of useful information of the noisy text in its intended form instead of filtering them out to analyze SM text better. The proposed TVH performs context-aware text normalization based on intended meaning to avoid the wrong word substitution. We integrate the TVH with state-of-the-art (SOTA) deep-learning-based text analysis methods to enhance their performance for noisy SM text data. The proposed scheme shows promising improvement in the text analysis of informal SM text in terms of precision, recall, accuracy, and F1-score in simulation.