bluuebunny/crossref_metadata_2025 huggingface dataset:
PlanteSaine: An Artificial Intelligent Empowered Mobile Application for Pests and Disease Management for Maize, Tomato, and Onion Farmers in Burkina Faso
Authors: Appiah Obed , Hackman Kwame Oppong , Diallo Belko Abdoul Aziz , Ogunjobi Kehinde O. , Diakalia Son , Valentin Ouedraogo , Abdoul-Karim Damoue , Dabire Gaston
Published: July 2024

This study presents PlanteSaine, a novel mobile application powered by Artificial Intelligence (AI) models explicitly designed for maize, tomato, and onion farmers in Burkina Faso. Agriculture in Burkina Faso, like many developing nations, faces substantial challenges from plant pests and diseases, posing threats to both food security and economic stability. PlanteSaine addresses these challenges by offering a comprehensive solution that provides farmers with real-time identification of pests and diseases. Farmers capture images of affected plants with their smartphones, and PlanteSaine’s AI system analyzes these images to provide accurate diagnoses. The application’s offline functionality ensures accessibility even in remote areas with limited Internet connectivity, while its messaging feature facilitates communication with agricultural authorities for guidance and support. Additionally, PlanteSaine includes an emergency alert mechanism to notify farmers about pest and disease outbreaks, enhancing their preparedness to deal with these threats. An AI-driven framework, featuring an image feature extraction phase with EfficientNetB3 and an artificial neural network (ANN) classifier, was developed and integrated into PlanteSaine. The evaluation of PlanteSaine demonstrates its superior performance compared to baseline models, showcasing its effectiveness in accurately detecting diseases and pests across maize, tomato, and onion crops. Overall, this study highlights the potential of PlanteSaine to revolutionize agricultural technology in Burkina Faso and beyond. Leveraging AI and mobile computing, PlanteSaine provides farmers with accessible and reliable pest and disease management tools, ultimately contributing to sustainable farming practices and enhancing food security. The success of PlanteSaine underscores the importance of interdisciplinary approaches in addressing pressing challenges in global agriculture
Responses of Soil Enzymes Activities to Sprinkler Irrigation and Differentiated Nitrogen Fertilization in Barley Cultivation
Authors: Siwik-Ziomek Anetta , Kuśmierek-Tomaszewska Renata
Published: July 2024

Our study aimed to assess the impact of sprinkler irrigation on the activity of selected soil enzymes in terms of nitrogen metabolism and oxidation–reduction processes in soil with different doses of inorganic nitrogen fertilizers. An Alfisol was sampled from an experimental field of spring barley within the University Research Center in the central part of Poland, namely the village of Mochełek with a moderate transitory climate, during the growing seasons of 2015–2017. The soil resistance (RS) was derived to recognize the resistance enzymes during drought. In the maturity phase, nitrate reductase activity was 18% higher in irrigated soil and the activities of other enzymes were higher than in the non-irrigated plots by 25% for dehydrogenase, 22% for peroxidase, 33% for catalase, and 17% for urease. The development phase in the barley influenced nitrate reductase activity. Enzymatic activities changed throughout the research years. During the maturity stage, a lower ammonium nitrogen content in the soil resulted from a higher spring barley uptake due to drought stress. Irrigation probably contributed to increased leaching of nitrate in the soil. The highest index of resilience was found in the soil catalase activity.
Lipidomics in Plants Under Abiotic Stress Conditions: An Overview
Authors: Henschel Juliane Maciel , Andrade Antônio Nunes de , dos Santos Josefa Bruna Lima , da Silva Rodrigo Ribeiro , da Mata Djair Alves , Souza Tancredo , Batista Diego Silva
Published: July 2024

Lipids are ubiquitous macromolecules that play essential roles in several metabolic processes in plants, such as primary and secondary metabolism, energy storage, and lipid signaling, also being major constituents of membranes. Considering their importance, lipid contents, proportion, and composition are widely modulated in response to environmental conditions, which is even more important under unfavorable conditions such as abiotic stresses. In recent years, technological advances have allowed for the analysis of the global lipid profile, also known as lipidomics, which has emerged as a powerful tool for the comprehensive analysis of the modulation and roles of lipids under different conditions. This review provides a current overview of plant lipidomics research, covering the different lipid classes found in plants, analytical techniques, and the main lipid-related responses under temperature, water, salt, alkali, heavy metal, nutrient deficiency, light, and oxidative stress.
Pasture Performance: Perspectives on Plant Persistence and Renewal in New Zealand Dairy Systems
Authors: Cartmill Andrew D. , Donaghy Daniel J.
Published: July 2024

Pasture systems dominate the landscape of Aotearoa, New Zealand, and are an integral component of sustainable and resilient livestock production. Predicting the response, performance, and dynamics of pasture species and adapting management practices is key to the long-term economic and environmental sustainability and resilience of the agricultural sector. However, there is limited information on the long-term productivity, performance, and persistence of forage cultivars and species for pasture production systems, particularly when linked to grazing and animal performance. Here, we sought to reduce scientific uncertainty, inform modelling efforts, and contribute to a predictive framework for understanding pasture performance, persistence, and renewal. Inter-annual pasture renewal (direct drilling and cultivation) rates vary by region and year, reflecting both opportunity and problem-based drivers, with the highest pasture renewal rates in Waikato and Canterbury on the North and South Island, respectively.
Optimization of Plant Density and Harvest Time to Maximize Volatile Oil Accumulation in Two Aromatic Plants
Authors: Zhang Yu , Xu Hongliang , Huang Yang , Xia Pengguo
Published: July 2024

Volatile oil, mainly extracted from aromatic plants, is widely used in medical, cosmetics, and other industries for its disinfection, sterilization, antioxidant, and other effects. The yield of volatile oil was influenced by plant density and harvest time. Here, two common aromatic plants, Perilla frutescens and Ysphania ambrosioide, were taken as examples to investigate the effects of plant density and harvest time on plant yield-related traits, content, and yield of volatile oil, and the oil yield was determined by steam distillation. Results showed that plant density and harvest time significantly affected the yield and quality of the two plants (p < 0.05). Increasing plant density significantly increased the dry yield and leaf dry yield of P. frutescens, while the dry yield and volatile oil content and yield of Y. ambrosioides significantly decreased, and the maximum value exhibited was at the plant density of 80 cm × 80 cm. In P. frutescens, the maximum volatile oil content was obtained at the plant density of 60 cm × 60 cm, and the maximum volatile oil yield was obtained at the plant density of 30 cm × 30 cm. The yield and volatile oil yield of these two plants increased first and then decreased with the extension of harvest time. The maximum of P. frutescens was in mid-September, and that of Y. ambrosioides was in late October to early November. Our study provides new data for improving plant volatile oil yield and commercial value by optimizing planting density and harvest time and highlights the importance of optimizing planting management mode.
Arbuscular Mycorrhizal Fungal Interacted with Biochar and Enhanced Phosphate-Solubilizing Microorganism Abundance and Phosphorus Uptake in Maize
Authors: Meng Lingbo , Cheng Zeyu , Wang Yingning , Li Shumin , Clarke Nicholas
Published: July 2024

In agricultural production, it is crucial to increase the availability of phosphorus (P) in cultivated soil to solve the P limitation. Arbuscular mycorrhizal fungi (AMF) have been proven to promote crop nutrient absorption effectively, while biochar can lead to improvements in soil properties. However, the possible synergistic effect of AMF and biochar on P uptake by crops as well as its underlying mechanisms are unclear. In this study, we conducted a pot experiment to explore the effects of biochar and AMF (Glomus etunicatum) on the community of rhizospheric phosphate-solubilizing microorganisms (PSMs) of maize (Zea mays L. Xianyu-335) using metagenomic methods. The experiment used 0 mg P2O5 g·kg−1 soil (P0) and 30 mg P2O5 g·kg−1 soil (P30) application rates. Each P application rate included 0 (NC), 20 g·kg−1 biochar (BC) addition, inoculation AMF, and without AMF treatments (NM) for a total of eight treatments. During the experiment, both the P uptake and the biomass of maize were measured. The study found that the combination of AMF and biochar significantly increased the mycorrhizal colonization rate of maize roots, regardless of P application level. It was observed that the P uptake by maize was significantly increased when exposed to a combination of AMF and biochar. The increase in P uptake in P0 treatments was 67% higher than the sum of the effects of biochar and AMF inoculation alone. The increase was only 35% higher in P30 treatments, demonstrating a substantially higher interactive effect under P0 than under P30 conditions. The AM-BC treatments significantly increased the abundance of Streptomyces, Bacillus, and Pseudomonas, genera that are known to contain PSMs. In addition, the abundance of genes related to P-cycling (gcd, phoD, and ugpQ) in PSMs increased significantly by 1.5–1.8 times in AM-BC treatments compared with NM-BC and AM-NC treatments under P0 conditions. This increase was significantly and positively correlated with the P uptake. Overall, the results suggest that biochar can help AMF colonize the roots, increasing the functional roles of PSMs in the rhizosphere, which in turn promotes P uptake and biomass in maize. This study provides a new way to improve P-use efficiency and reduce the need for P-fertilizer application in agricultural production.
Connecting the Dots between GmPERK-1 and Enhanced Grain Weight in Glycine max
Authors: Lu Yanming , Irshad Ahsan , Rehman Shoaib Ur , Wang Yan , Zhou Boqian , Jin Hua
Published: July 2024

Large and distinct families of receptor-like kinases (RLKs) play elemental roles in many fundamental processes of plants. The proline-rich extensin-like receptor kinase (PERK) family is one of the most pivotal classes of RLKs. To date, there have been no comprehensive or published studies conducted on the PERK gene family in Glycine max. This research aimed to characterize the role of the PERK gene family in cultivated soybean using a systematic array of bioinformatic and experimental approaches. We identified 16 PERK members in G. max through local BLASTp, using PERK members from Arabidopsis thaliana as a query. Tissue expression of genes, predicted via tissue specific expression analysis from the soybean database “SoyBase”, revealed that these PERK genes exhibit differentiated expression patterns in various plant organs. The gene structure was predicted via Gene Structure Display Server (GSDS). Phylogeny was demonstrated through an evolutionary tree employing the neighbor-joining method. Subcellular localization of proteins was identified via “Softberry” and cis-acting elements were identified through PlantCARE. The KASP (Kompetitive Allele Specific PCR (KASP)) marker was developed for the GmPERK-1-C and GmPERK-1-T allele, targeting position 167 nt in the CDS region. Genotyping results indicated that GmPERK-1 exhibits promising potential for utilization in molecular breeding programs for soybean to increase crop yield. Collectively, our findings indicate that G. max accessions harboring the GmPERK-1-C allele exhibit significantly higher thousand grain weight compared to accessions carrying the GmPERK-1-T allele. This research enhances the understanding of the molecular roles of PERK genes in G. max, providing valuable insights for the utilization of favorable genetic variations in soybean molecular breeding programs.
Microbiome Analysis Revealed the Effects of Environmental Factors on the Presence of Toxigenic Fungi and Toxin Production in Rice Grains
Authors: Zhang Fengmin , Cao Zhenzhen , Zhao Xiaohua , Yan Qing , Guan Meiyan , Chen Mingxue , Lin Xiaoyan
Published: July 2024

Fungal contamination in rice and mycotoxins present significant challenges to both rice quality and food safety. However, there is a dearth of comprehensive research on the compositional and structural changes within fungal colonies in rice, particularly in typical rice-producing regions, as well as their underlying influencing factors. In this study, a comprehensive analysis of fungal taxa in rice grains was conducted using amplicon sequencing and bioinformatics methods on 99 rice samples collected in three major rice-producing regions in China: Northeast Plain (NP), Yangtze River Basin (YR), and Southeast Coastal Area (SC). A total of 6,019,722 fungal ITS sequences were obtained with an average sequence length of 235 base pairs, and effective ASVs (2014) accounted for approximately 97.58% of the total ASVs (2064). The fungal community diversity in rice grains exhibited significant variations across the three regions, with deterministic processes playing a predominant role in shaping the ecological dynamics of fungal taxa. Among the core microbiota (92 shared ASVs), the first five species (Alternaria, Fusarium, Curvularia, Epicoccum, and Ustilaginoidea) accounting for a proportion greater than 5% had been reported as potential pathogens for plants. Geographical variations in fungal community composition were evident, with a significantly higher number of shared populations observed between YR and CS regions compared to those in the NP region. Nutrient elements and climatic conditions were the internal and external driving factors of rice fungal community composition. Additionally, notable regional variations in fungal functionality were observed. The findings have significant implications for gaining a comprehensive understanding of the distribution patterns of fungal communities in the major rice-producing regions in China. Additionally, it provides valuable insights into controlling key influencing factors to effectively reduce the occurrence of toxin-producing fungi and mitigate the associated risks related to mycotoxin contamination, thereby contributing to improved risk management and assessment.
Nutritional Partitioning among Sympatric Ungulates in Eastern Tibet
Authors: Luo Zhengwei , Pei Chao , Zhang Haonan , Wang Yichen , Zhang Baofeng , Hu Defu
Published: July 2024

Wild ungulates play crucial roles in maintaining the structure and function of local ecosystems. The alpine musk deer (Moschus chrysogaste), white-lipped deer (Przewalskium albirostris), and red serow (Capricornis rubidus) are widely distributed throughout the Nyenchen Tanglha Mountains of Tibet. However, research on the mechanisms underlying their coexistence in the same habitat remains lacking. This study aimed to investigate the mechanisms underlying the coexistence of these species based on their dietary preferences through DNA barcoding using the fecal samples of these animals collected from the study area. These species consume a wide variety of food types. Alpine musk deer, white-lipped deer, and red serow consume plants belonging to 74 families and 114 genera, 62 families and 122 genera, and 63 families and 113 genera, respectively. Furthermore, significant differences were observed in the nutritional ecological niche among these species, primarily manifested in the differentiation of food types and selection of food at the genus level. Owing to differences in social behavior, body size, and habitat selection, these three species further expand their differentiation in resource selection, thereby making more efficient use of environmental resources. Our findings indicate these factors are the primary reasons for the stable coexistence of these species.
Rumen Microbiota Transplantation Alleviates Gossypol Diet-Induced Reproductive, Liver, and Intestinal Damage in Male Mice
Authors: Zhang Chen , Lu Wenguang , Liu Huiru , Shen Lingwei , Zhu Mengfan , Zhou Tangtang , Zhang Ling , Xiao Dingfu , Chen Lijuan
Published: July 2024

Ruminants exhibit stronger tolerance to gossypol, an anti-nutritional factor, compared to monogastric animals. We transplanted Hu sheep rumen microbiota into male mice to investigate the role of rumen microbiota in animal gossypol tolerance. Thirty specific-pathogen-free (SPF) male C57BL/6 mice were randomly divided into three groups: normal diet (CK group), gossypol diet (FG group), and rumen microbiota transplantation (FMT group, gossypol diet). The pathological changes in the liver and small intestine of the mice, the organ coefficient, and sperm parameters were analyzed. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the blood and lactate dihydrogen-X (LDH-X) levels in the testicular tissue were also measured. The results showed that body weight, feed intake, sperm concentration, sperm motility, and LDH-X levels in the FMT group increased (p < 0.05) compared with the FG group, while the enzyme activities of ALT, AST, and AST/ALT decreased (p < 0.05). In the FMT group, the injury to liver cells was alleviated, the structure of the small intestine was intact, and the villus height and the ratio of villus height to crypt depth (V/C) were higher than those in the FG group (p < 0.05). And there were no differences in various organ coefficients and sperm deformity rates among the three groups (p > 0.05), but compared with the FG group, mice in the FMT group showed tendencies closer to those in the CK group. Rumen microbiota transplantation relieved the reproductive toxicity and liver damage induced by gossypol in male mice and improved the tolerance of recipient animals to gossypol. Additionally, rumen microbes improved the intestinal structural integrity of recipients.
Heat Shock Related Protein Expression in Abdominal Testes of Asian Elephant (Elephas maximus)
Authors: Sato Yoko , Tharasanit Theerawat , Thitaram Chatchote , Somgird Chaleamchat , Mahasawangkul Sittidet , Thongtip Nikorn , Chatdarong Kaywalee , Tiptanavattana Narong , Taniguchi Masayasu , Otoi Takeshige , Techakumphu Mongkol
Published: July 2024

The abdominal testes of Asian elephants show normal spermatogenesis. Heat shock in cryptorchid testes elevates heat shock factor (HSF) expression, leading to germ cell apoptosis, while increased heat shock proteins (HSPs) levels provide protection. To investigate how heat shock affects elephant spermatogenic cells, focusing on heat shock-related molecules and the cell death mechanism, immunohistochemistry and TUNEL staining were employed to assess the immunoexpression of several heat shock-related molecules and the status of apoptosis in elephant fibroblasts (EF) induced by heat shock stimulus. Additionally, the immunoexpression of heat shock-related molecules and cell proliferation status in the elephant spermatogenic cells. Our finding indicated that heat shock-induced HSF1 immunoexpression in EF leads to apoptosis mediated by T-cell death-associated gene 51 (TDAG51) while also upregulating HSP70 to protect damaged cells. In elephant spermatogenic cells, immunostaining revealed a predominance of proliferating cell nuclear antigen (PCNA)-positive cells with minimal TDAG51- and TUNEL-positive cells, suggesting active proliferation and apoptosis suppression during normal spermatogenesis in the abdominal testis. Interestingly, spermatogonia co-immunoexpressed HSF1 and HSP90, potentially reducing apoptosis through protective mechanisms different from those observed in other mammals. Spermatogenic cells did not show immunolocalisation of HSP70, and hence, it may not contribute to protecting the spermatogonia from heat shock because the transcriptional activity of HSF1 is suppressed by HSP90A binding. This study provides insight into the specific heat shock response and defence mechanisms in elephant spermatogenic cells and may contribute to our understanding of species-specific adaptation to environmental stresses of the testis.
Complete Thoracic Ectopia Cordis in Two Lambs
Authors: Cerqueira Liz de Albuquerque , Mâcedo Isabel Luana de , Sousa Davi Emanuel Ribeiro de , Amorim Haiane Arruda Luz , Borges José Renato Junqueira , Ximenes Fábio Henrique Bezerra , Câmara Antonio Carlos Lopes , Castro Márcio Botelho de
Published: July 2024

Cardiac congenital defects related to inheritance and teratogenesis have been reported in veterinary species and humans worldwide. Among these, ectopia cordis (EC), characterized by an externalized heart through a cleft, is extremely rare in sheep. This report presents the diagnostic features of two cases of complete thoracic EC in newborn lambs. Clinical findings in the lambs, aside from the EC, were unremarkable. Both animals exhibited exteriorized hearts without pericardial coverage, delineated in the thoracic cleft by a fibrous ring of the pericardium and adjacent skin. Histologically, the epicardium was thickened by fibrous tissue in both lambs, with one animal also showing marked edema, hemorrhage, and neutrophilic inflammatory infiltration. The prognosis of EC in the lambs of this study was poor, with fatal outcomes despite attempts at surgical correction.
Effects of Personality and Behavioral Syndromes on Competition for Social Hierarchical Status in Anemonefish Amphiprion clarkii
Authors: Wu Lisheng , Deng Shunyun , Tang Wei , Zhang Sipeng , Liang Feng , Ding Shaoxiong
Published: July 2024

In this study, the behavioral ethogram of Amphiprion clarkii during the growth phase prior to sexual differentiation was summarized based on behavioral observations in three social environments. These behaviors can be classified into four categories: in addition to normal behaviors, the other three categories of behaviors—threatening, agonistic, and appeasing behaviors—represent different intentions in interactions with other individuals. Subsequently, the personalities of each individual were assessed by testing their reactions to intruders. These individuals mainly exhibited two distinct personality types: bold-aggressive and shy-submissive. In pairing experiments, the interactive behaviors of the anemonefish were observed in pairing combinations of different body sizes and personalities. The impact of personality on the establishment of a stable social hierarchy was confirmed by significant differences in the success rates of different pairing combinations, with the frequency of appeasing behaviors being the main factor influencing the success rate. Our results suggested that in natural waters, when juvenile individuals migrate among host anemones, shy-submissive individuals are more likely to be accepted due to their appeasing behaviors towards larger individuals, thus avoiding the risk of being attacked and bitten, and benefiting the survival of the individual. Conversely, bold-aggressive individuals are more likely to be driven away to another host anemone due to their unwillingness to settle for a lower-ranked status, thereby contributing to population dispersal and increasing opportunities for gene exchange between populations.
Isavuconazole Pharmacokinetics in Critically Ill Patients: Relationship with Clinical Effectiveness and Patient Safety
Authors: Martín-Cerezuela María , Maya Gallegos Cristina , Marqués-Miñana María Remedios , Broch Porcar María Jesús , Cruz-Sánchez Andrés , Mateo-Pardo Juan Carlos , Peris Ribera José Esteban , Gimeno Ricardo , Castellanos-Ortega Álvaro , Poveda Andrés José Luis , Ramírez Galleymore Paula
Published: July 2024

Isavuconazole is used to treat fungal infections. This study aims to describe isavuconazole pharmacokinetics in critically ill patients and evaluate their relationship with clinical efficacy and patient safety. We conducted a prospective, observational study in patients treated with intravenous isavuconazole. Samples were collected at predose (Cmin), 1 h (Cmax) and 12 h (C50) after the last dose. The plasma concentration was determined by high-performance liquid chromatography. The relationship between plasma concentration and clinical and microbiological outcomes and safety was evaluated. The influence of covariates (age, sex, weight, SAPS3, creatinine, liver enzymes and extracorporeal devices: continuous renal replacement therapy (CRRT) and extracorporeal membrane oxygenation (ECMO)) was analysed. Population pharmacokinetic modelling was performed using NONMEN®. A total of 71 isavuconazole samples from 24 patients were analysed. The mean Cmin was 1.76 (1.02) mg/L; 87.5% reached the optimal therapeutic target and 12.5% were below 1 mg/L. Population pharmacokinetics were best described by a one-compartment model with first-order elimination. No factor had a significant impact on the plasma concentration or pharmacokinetic parameters. Thus, isavuconazole could be safely used in a critically ill population, even in those treated with CRRT and ECMO, from a pharmacokinetic standpoint. Therefore, routine therapeutic drug monitoring may not be strictly necessary in daily clinical practice.
Antimicrobial Resistance in Equines: A Growing Threat to Horse Health and Beyond—A Comprehensive Review
Authors: Kabir Ajran , Lamichhane Bibek , Habib Tasmia , Adams Alexis , El-Sheikh Ali Hossam , Slovis Nathan M. , Troedsson Mats H. T. , Helmy Yosra A.
Published: July 2024

The equine industry holds substantial economic importance not only in the USA but worldwide. The occurrence of various infectious bacterial diseases in horses can lead to severe health issues, economic losses, and restrictions on horse movement and trade. Effective management and control of these diseases are therefore crucial for the growth and sustainability of the equine industry. While antibiotics constitute the primary treatment strategy for any bacterial infections in horses, developing resistance to clinically important antibiotics poses significant challenges to equine health and welfare. The adverse effects of antimicrobial overuse and the escalating threat of resistance underscore the critical importance of antimicrobial stewardship within the equine industry. There is limited information on the epidemiology of antimicrobial-resistant bacterial infections in horses. In this comprehensive review, we focus on the history and types of antimicrobials used in horses and provide recommendations for combating drug-resistant bacterial infections in horses. This review also highlights the epidemiology of antimicrobial resistance (AMR) in horses, emphasizing the public health significance and transmission dynamics between horses and other animals within a One Health framework. By fostering responsible practices and innovative control measures, we can better help the equine industry combat the pressing threat of AMR and thus safeguard equine as well as public health.
A Novel Bacitracin-like Peptide from Mangrove-Isolated Bacillus paralicheniformis NNS4-3 against MRSA and Its Genomic Insights
Authors: Sermkaew Namfa , Atipairin Apichart , Wanganuttara Thamonwan , Krobthong Sucheewin , Aonbangkhen Chanat , Yingchutrakul Yodying , Uchiyama Jumpei , Songnaka Nuttapon
Published: July 2024

The global rise of antimicrobial resistance (AMR) presents a critical challenge necessitating the discovery of novel antimicrobial agents. Mangrove microbes are valuable sources of new antimicrobial compounds. This study reports the discovery of a potent antimicrobial peptide (AMP) from Bacillus paralicheniformis NNS4-3, isolated from mangrove sediment, exhibiting significant activity against methicillin-resistant Staphylococcus aureus (MRSA). The AMP demonstrated a minimum inhibitory concentration ranging from 1 to 16 µg/mL in the tested bacteria and exhibited bactericidal effects at higher concentrations. Structural analysis revealed a bacitracin-like configuration and the peptide acted by disrupting bacterial membranes in a time- and concentration-dependent manner. The AMP maintained stability under heat, proteolytic enzymes, surfactants, and varying pH treatments. The ten biosynthetic gene clusters (BGCs) of secondary metabolites were found in the genome. Detailed sequence comparison of the predicted bacitracin BGC indicated distinct DNA sequences compared to previously reported strains. Although the antibiotic resistance genes were found, this strain was susceptible to antibiotics. Our findings demonstrated the potential of Bacillus paralicheniformis NNS4-3 and its AMP as a promising agent in combating AMR. The genetic information could be pivotal for future applications in the healthcare industry, emphasizing the need for continued exploration of marine microbial diversity in drug discovery.
Sport and Physical Activity Participation by Weight Groups in School-Aged Hungarian Children
Authors: Uvacsek Martina , Wágner Bernadett , Simkó Georgina
Published: July 2024

The aim of this study was to compare physical activity and organized sport participation survey data between different weight classifications in children. In the cross-sectional online data collection, 677 parents (42.8 ± 6.4 years old) provided information about 677 children (10.9 ± 2.5 years old). On average, 77% of the children achieved 60 min of MVPA/day and 63% participated in a club or organized sport. Most of them (63%) were in the healthy weight category, 14% of the children were underweight, and 22% of the children were overweight or obese. The parental support for physical activity was 83%. Club or organized sport participation (OR = 0.56 CI: 0.34–0.91; p < 0.02) and a parent with a higher educational level (OR = 0.25 CI:0.14–0.43; p < 0.01) were found to be preventive for obesity. Football was the most popular and highly chosen sport activity. Regarding sport activities with parents, cycling, walking, and football were the most popular, independently of the weight classification of the child. The overweight and obese children had almost the same participation rate in sport activities than others. Increased physical activity might be explained by the daily physical education in schools and governmental support. Further studies and measures are needed to prevent overweight and obesity in children of parents with a lower educational level.
Influences of Different Selective Laser Melting Machines on the Microstructures and Mechanical Properties of Co–Cr–Mo Alloys
Authors: Takaichi Atsushi , Kajima Yuka , Htat Hein Linn , Wakabayashi Noriyuki
Published: July 2024

Dental prostheses have been fabricated using various selective laser melting (SLM) machines; however, the impact of the type of machine on the microstructure and mechanical properties of Co–Cr–Mo alloys remains unclear. In this study, we prepared samples using two SLM machines (the small M100 and mid-sized M290) with different beam spot sizes (40 and 100 µm, respectively). The microstructures and tensile properties of the heated (1150 °C for 60 min) and as-built samples were evaluated. The grain sizes of the M100 samples were smaller than those of the M290 samples due to the small beam spot size of the M100 machine. Both heated samples exhibited recrystallized equiaxed grains; however, the amount of non-recrystallized grains remaining in the M290 sample exceeded that in the M100 sample. This suggests that the M100 samples recrystallized faster than the M290 samples after heating. The elongation of the M100 samples was higher than that of the M290 samples in the as-built and heated states, owing to the smaller grain size of the M100 samples. A comparison of the M100 and M290 SLM machines indicated that the M100 was suitable for producing dental prostheses owing to its good elongation and rapid recrystallization features, which shorten its post-heat-treatment duration.
Reservoir Characteristics of Marine–Continental Transitional Taiyuan Formation Shale and Its Influence on Methane Adsorption Capacity: A Case Study in Southern North China Basin
Authors: Jiang Wei , Hu Yang
Published: July 2024

To further study the reservoir characteristics and adsorption capacity of the Taiyuan Formation shale in the South North China Basin (SNCB), the pore structure and adsorption capacity of shale are discussed using various analysis tests, including elemental geochemistry, organic geochemistry, mineral composition, low-temperature nitrogen adsorption (LTNA), and methane adsorption experiments. The results indicate that the Taiyuan Formation shale formed in a poor oxygen and anaerobic sedimentary environment in still water. The average value of total organic carbon (TOC) content is 2.37%. The organic matter type mainly consists of type III kerogen. The vitinite reflectance (Ro) ranges from 3.11% to 3.50%. The clay mineral content varies greatly, averaging at 40.7%, while the quartz content averages at 37.7%. The Taiyuan Formation shale mainly develops interparticle (InterP) pores, followed by organic pores, intraparticle (IntraP) pores, solution pores, and microfractures. BET specific surface area (SSA) is between 9.47 m2/g and 22.14 m2/g, while pore volume (PV) ranges from 0.0098 cm3/g to 0.022 cm3/g, indicating favorable conditions for shale gas storage. According to the results of the CH4 adsorption experiment, Langmuir volume from Taiyuan Formation shales exhibits 1.35~4.30 cm3/g, indicating excellent adsorption capacity. TOC content shows a positive correlation with both Langmuir volume and BET SSA from Taiyuan Formation shales, suggesting that TOC plays a crucial role in controlling microscopic pores and gas adsorption capacity. Organic matter enhances the shale adsorption capacity by providing abundant pore SSA. Due to formation compaction, the pore size of clay minerals decreases, leading to an increase in pore SSA, while kaolinite exhibits weak hydrophilic ability. Consequently, with the increase in clay minerals and kaolinite content, the shale adsorption capacity is enhanced to a certain extent. However, an increase in the carbonate mineral content may result in a decrease in the proportion of clay minerals, therefore reducing the CH4 adsorption capacity of shale.
Semantic Kansei Engineering Approach for Game Controllers and Design Improvement
Authors: González A. G. , López O. , Pizarro M. , Vázquez J. F.
Published: July 2024

The entertainment industry undergoes constant changes, and video games, along with the ways people enjoy them, evolve dynamically. In this context, there arises a need to innovate in areas such as user–game interaction and the design of conventional controllers to keep up with these transformations. This study has found that the design requirements of the external geometry of video game controllers significantly impact users’ purchasing decisions, who often rely on their sensations and emotions. The primary aim of this research has been to demonstrate that it is possible to develop a specific methodology to establish design guidelines that evaluate geometry and ergonomic aspects, considering users’ emotional needs. To achieve this goal, the Kansei methodology has been employed, an engineering discipline that focuses on product design based on emotional interaction and user sensations when using them. Through advanced statistical techniques such as principal component analysis and linear regression, the set of observations has been reduced and a robust predictive model has been developed. Following this methodological approach, surveys and field studies have been conducted, thus differentiating the project from a conventional engineering approach in various stages. Special attention has been paid to the tactile part of the controller, especially its casing. After thorough analysis, the redesigned casing, incorporating improvements derived from statistical analyses, has been compared to existing market models. The results obtained show a significant improvement compared to existing market models, particularly highlighting enhancements in functional characteristics and ergonomic design, enabling comfortable use for individuals with different hand sizes and preventing the grip surface from being slippery. In summary, this work supports the effectiveness of the Kansei methodology as an approach to adapting product design to users’ perceptions and emotional needs.
Timing Performance Testing and Regularity Analysis of eLoran System
Authors: Di Jianchen , Fu Jun , Xu Bing , Wu Miao , Liu Liang , Jin Xianzhou
Published: July 2024

Accurate time synchronisation is critical in modern communication, navigation, and scientific research. In this context, the eLoran receiver, which is an advanced timing device, has attracted increasing scholarly attention. This study aims to comprehensively analyse the performance and potential applications of eLoran systems for timing monitoring and to specifically explore the relevant indices of two eLoran receivers. To this end, we evaluated the performance of these receivers in receiving time signals through both simulated and empirical data and conducted a regularity analysis to uncover their potential value in practical applications. The findings demonstrate that the eLoran receiver excels at timing monitoring and provides highly accurate time information. An analysis of the timing performance of the eLoran system improved its accuracy and integrity.
Removal of Reactive Yellow 86 from Synthetic Wastewater in Lab-Scale Constructed Wetlands Planted with Cattail and Papyrus
Authors: Yamamoto Akihiro , Eguchi Hiroki , Soda Satoshi
Published: July 2024

Synthetic wastewater was treated in lab-scale constructed wetlands (CWs) in sequencing batch mode to evaluate roles of aquatic plants for removing an azo dye: Reactive Yellow 86 (RY86). Under hydraulic retention time (HRT) of 5 days, removal by unplanted CWs was less than 20% for RY86 of 10–50 mg/L. The CWs planted with cattail and papyrus demonstrated RY86 removal of 50–68% and 73–84%, respectively. For wastewater containing 50 mg/L RY86, removal in the unplanted CW was <12%, even under a 15 day HRT, although it was 22–71% in CWs planted with cattail and 34–81% in CWs planted with cattail, with increasing values under HRTs of 1 day to 15 days. Both cattail and papyrus grew well, extending their roots in the CWs for 90 days. RY86-decolorizing microorganisms were detected in CW effluent. Overall, RY86 removal was positively correlated with evapotranspiration in the CWs, indicating the plant uptake as the main removal mechanism. Papyrus and cattail, especially the former, are suitable plants for CWs intended to treat RY86-containing wastewater.
A Network Analysis-Based Approach for As-Built BIM Generation and Inspection
Authors: Hu Wei , Xie Zhuoheng , Cai Yiyu
Published: July 2024

With the rapid advancement in Building Information Modelling (BIM) technology to strengthen the Building and Construction (B&C) industry, effective methods are required for the analysis and improvement of as-built BIM, which reflects the completed building project and captures all deviations and updates from the initial design. However, most existing studies are focused on as-designed BIM, while the analysis and inspection of as-built BIM rely on labour-intensive visual and manual approaches that overlook interdependent relationships among components. To address these issues, we propose a network analysis-based approach for managing and improving as-built BIM. Networks are generated from geometric attributes extracted from Industry Foundation Classes (IFC) documents, and network analytical techniques are applied to facilitate BIM analysis. In addition, a practical dataset is utilised to verify the feasibility of the proposed approach. The results demonstrate that our method significantly enhances the analysis and comparison of as-built BIM from model analysis and matching. Specifically, the innovative contribution leverages global information and interdependent relations, providing a more comprehensive understanding of the as-built BIM for effective management and optimisation. Our findings suggest that network analysis can serve as a powerful tool for structure and asset management in the B&C industry, offering new perspectives and methodologies for as-built BIM analysis and comparison. Finally, detailed discussion and future suggestions are presented.
Fruiting Characteristics and Molecular-Assisted Identification of Korla Fragrant Pear Bud Mutation Materials
Authors: Yang Xian’an , Zhang Cuifang , Sun Haichang , Wang Shiwei , Cui Yutong , Zhao Long
Published: July 2024

Korla fragrant pear is a high-quality local pear variety native to Xinjiang, China. Currently, the low fruit-setting rate and low calyx shedding rate problems in Korla fragrant pears have been highlighted, which seriously affect the fruit yield and quality. It is of great significance to research the fruiting characteristics and molecular-assisted identification of Korla fragrant pear bud mutation materials for enriching the germplasm resources of Korla fragrant pear. In this research, a natural pollination group (YB) of Korla fragrant pear bud mutation materials and a natural pollination group (CK) of Korla fragrant pears were established. On the fruiting characteristics, the fruit-setting rate and calyx-removal rate of the two groups were investigated. In terms of fruit quality, the fruit shape index, fruit specific gravity, soluble solids content, sugar:acid ratio, soluble sugar content, and other indicators were measured. For the anatomical structure of the calyx tube, the detachment cells were observed. The formation time of the two groups of detached cells was compared. In the GBS simplified genome sequencing, a phylogenetic tree was constructed based on the obtained SNP sites. A principal component analysis, population genetic structure analysis, and genetic diversity index analysis were carried out. In the aspect of SSR molecular marker identification, the SSR types were counted. Polyacrylamide gel electrophoresis was performed. The results demonstrate the following: (1) the fruit-setting rate (30.87%) and calyx-removal rate (68.11%) in the YB group were significantly higher than those in the CK group (19.37%) and the calyx-removal rate (55.18%). (2) There was no significant difference in fruit quality indexes, such as average fruit weight (127.10–130.00 g) and soluble sugar content (9.47–9.56%) between the two groups. (3) Abscission-layer cells were observed at 2, 4, 6, 8, and 10 h after calyx tube discoloration in the YB group and at 48, 72, and 96 h after calyx tube discoloration in the CK group. (4) The genetic background of the YB group and the CK group was similar at the GBS level, but there were differences at the DNA level. This research finally shows that Korla fragrant pear bud mutation material is a good germplasm resource. This germplasm resource can promote the structural optimization of Korla fragrant pear varieties and the healthy development of the industry.
Research and Simulation Analysis of Fuzzy Intelligent Control System Algorithm for a Servo Precision Press
Authors: He Yanzhong , Luo Xiang , Wang Xingsong
Published: July 2024

With the rapid development of the manufacturing industry toward intelligence and flexibility, traditional mechanical presses are unable to meet the increased stamping requirements due to the difficulties in achieving variable speed control and changing slide motion trajectories. Servo presses, driven directly by servo motors, can realize the flexible control of press movement and have become the trend in the industry for future development. The independent research and development of servo press control systems has become a popular topic and challenge in the domestic press industry. This paper proposes a new type of press transmission mechanism suitable for high-precision stamping, analyzes the working principle of its transmission mechanism, and provides a detailed analysis and discussion on the main research methods of fuzzy intelligent control for servo presses, including fuzzy model and basic control algorithms, fuzzy modeling and control of nonlinear systems, fuzzy predictive control, and stability analysis of fuzzy control systems. Based on the principle of the control scheme, a closed-loop control system block diagram and mathematical model for servo high-precision presses are established. Taking the physical prototype parameters of the STSZG1-250 as an example, the control system is dynamically simulated and verified using Simulink modules. Finally, their role and effects in intelligent control, and prospects for the development of fuzzy intelligent control, are discussed.
Evaluation of Safety, Patient Perception and Efficacy of a New Cymenol-Based Mouth Rinse Formulation: A Randomized Clinical Trial
Authors: Araoz Ana , Figuero Elena , Serrano Jorge , Roldán Silvia , Alonso Bettina , Sanz Mariano , Herrera David
Published: July 2024

The aim of this study was to evaluate a newly formulated mouth rinse containing cymenol in patients undergoing supportive periodontal care in terms of safety (primary outcome) and the impact on dental biofilm and gingival inflammation (secondary outcomes). This research was designed as a pilot, controlled, randomized, parallel, triple-blinded, single-center, clinical trial of a 12-week duration. Adverse events and product perception were assessed by a questionnaire. Clinical, patient-reported outcomes (PROs), compliance, tooth staining, dentin hypersensitivity and microbiological variables were also evaluated. Student T, Mann–Whitney-U and Chi-square tests were applied. Thirty participants (15 per group) were included, randomized and followed for 12 weeks. No adverse events were reported. The questionnaire showed an overall rating of 7.2 (out of 10) in the experimental group and of 8.2 in the control group (p = 0.165) at 12 weeks. No statistically significant differences were observed in terms of gingival health, tooth staining, dentin hypersensitivity or microbiological outcomes between groups at baseline, 6- and 12-week visits. The adjuvant use of the new mouth rinse formulation proved to be as safe as the control product and no significant differences were observed in terms of clinical efficacy.
Reducing the Cooling Energy Demand by Optimizing the Airflow Distribution in a Ventilated Roof: Numerical Study for an Existing Residential Building and Applicability Map
Authors: Rincón-Casado Alejandro , Rodríguez Jara Enrique Ángel , Ruiz Pardo Alvaro , Salmerón Lissén José Manuel , Sánchez de la Flor Francisco José
Published: July 2024

This work presents a study of a ventilated hollow core slab system (VHCS) that obviates the need to completely replace the slab of an existing residential building. It is assimilated to a heat exchanger to allow its effectiveness to be studied as a function of the area and airflow rate. The balance between the energy consumed by the fan and the heat evacuated by the system is also studied through the use of the thermo-hydraulic performance factor (THPF), for which a series of cases were simulated by CFD following a methodology in which a configuration is achieved by means of the sequential analysis of cases in which both the thermal effectiveness and the THPF are maximized. The configuration chosen in this study was found to benefit from high airflow rates since, although this implies an increase in fan energy consumption, the increase in heat removed is proportionally greater. It has also been found that the design of the airflow distribution through the slab is of high importance as it affects both the heat exchanged with the slab and the pressure losses. An applicability map has been developed as a function of the temperature of the space below and the air temperature at the inlet of the ventilated roof. The heat flux per unit area that the studied envelope is able to evacuate is about 20 W/m2 K.