bluuebunny/crossref_metadata_2025 huggingface dataset:
Discharge Against Medical Advice in Traumatic Brain Injury: Follow-Up and Readmission Rate
Authors: Marcoux Judith , Alkutbi Mohammad , Lamoureux Julie , Feyz Mitra , Saluja Rajeet S. , de Guise Elaine
Published: May 2016

Background:Patients who leave hospital against medical advice (AMA) may be at risk of adverse health outcomes, medical complications, and readmission. In this study, we examined the characteristics of patients who left AMA after traumatic brain injury (TBI), their rates of follow-up visits, and readmission.Methods:We retrospectively studied 106 consecutive patients who left the tertiary trauma center AMA (1.8% of all admitted patients with a TBI). Preinjury health and social issues, mechanism of injury, computed tomography findings, and injury markers were collected. They were correlated to compliance with follow-up visits and unplanned emergency room (ER) visits and readmission rates.Results:The most prevalent premorbid health or social-related issues were alcohol abuse (33%) and assault as a mechanism of trauma (33%). Only 15 (14.2%) subjects came to follow-up visit for their TBI. Sixteen (15.1%) of the 106 subjects had multiple readmissions and/or ER visits related to substance abuse. Seven (6.6%) had multiple readmissions or ER visits with psychiatric reasons. Those patients with multiple readmissions and ER visits showed in higher proportion preexisting neurological condition (p=0.027), homelessness (p=0.012), previous neurosurgery (p=0.014), preexisting encephalomalacia (p=0.011), and had a higher ISS score (p=0.014) than those who were not readmitted multiple times.Conclusions:The significantly increased risks of multiple follow-up visits and readmission among TBI patients who leave hospital AMA are related to a premorbid vulnerability and psychosocial issues. Clinicians should target AMA TBI patients with premorbid vulnerability for discharge transition interventions.
Is there a role of pharmacogenomics in Africa
Authors: Matimba A. , Dhoro M. , Dandara C.
Published: May 2016

Pharmacogenomics has the potential of transforming clinical research and improving healthcare in sub-Saharan Africa (SSA). The role of African genome diversity and the opportunities for pharmacogenomics research are highlighted and will enable discovery of novel genetic mechanisms and validation of established markers. African genomics and biobank consortia will play an important role in building capacity for pharmacogenomics in SSA.
Cost-Effectiveness Analysis of Safety-Engineered Devices
Authors: Fukuda Haruhisa , Moriwaki Kensuke
Published: May 2016

OBJECTIVE

To estimate the cost-effectiveness of safety-engineered devices (SEDs) relative to non-SEDs for winged steel needles, intravenous catheter stylets, suture needles, and insulin pen needles.

DESIGN

Decision analysis modeling.

PARTICIPANTS

Hypothetical cohort of healthcare workers who utilized needle devices.

METHODS

We developed a decision-analytic model to estimate and compare the life-cycle costs and benefits for SED and non-SED needle devices. For this cost-effectiveness analysis, we quantified the total direct medical cost per needlestick injury, number of needlestick injuries avoided, and incremental cost-effectiveness ratio. Sensitivity analyses were performed to examine the robustness of the base-case analysis.

RESULTS

In the base-case analysis, we calculated the incremental cost-effectiveness ratios of SED winged steel needles, intravenous catheter stylets, suture needles, and insulin pen needles to be $2,633, $13,943, $1,792, and $1,269 per needlestick injury avoided, respectively. Sensitivity analyses showed that the calculated incremental cost-effectiveness ratio values for using SEDs did not fall below zero even after adjusting the values of each parameter.

CONCLUSION

The use of SED needle devices would not produce cost savings for hospitals. Government intervention may be needed to systematically protect healthcare workers in Japan from the risk of bloodborne pathogen infections.

Infect Control Hosp Epidemiol 2016;37:1012–1021
Wards, At-Large Systems and the Focus of Representation in Canadian Cities
Authors: Koop Royce , Kraemer John
Published: May 2016

Proponents of both ward and at-large systems agree that these systems of election play a role in shaping the representational foci (that is, who representatives seek to represent) of city councillors and, in so doing, affect the quality of local democracy. Canadian cities employ both ward and at-large systems of election, and therefore provide an opportunity to explore the relationship between elective systems and focus of representation. We draw on data derived from both 52 interviews with and a survey of councillors in Canadian cities to test the proposition that cities' systems of election influence the representational foci of councillors. We find strong evidence that ward systems are related to a representational focus on geographically defined neighbourhoods, whereas councillors in at-large systems report prioritizing representation of their cities as wholes.
STATISTICS AND INTELLIGENCE IN DEVELOPING COUNTRIES: A NOTE
Authors: Kodila-Tedika Oasis , Asongu Simplice A. , Azia-Dimbu Florentin
Published: May 2016

The purpose of this study is to assess the relationship between intelligence (or human capital) and the statistical capacity of developing countries. The line of inquiry is motivated essentially by the scarce literature on poor statistics in developing countries and an evolving stream of literature on the knowledge economy. A positive association is established between intelligence quotient (IQ) and statistical capacity. The relationship is robust to alternative specifications with varying conditioning information sets and control for outliers. Policy implications are discussed.
Use of bait containing triclabendazole against Fasciola gigantica in a herd of captive wild impala (Aepyceros melampus)
Authors: Phiri A.M. , Mudenda N.M. , Luwe M. , Phiri I.G.K.
Published: May 2016

Although the efficacy of triclabendazole treatment against Fasciola gigantica has been shown before in cattle and buffaloes, there appears to be no published report on the efficacy of triclabendazole in impala or other antelope species. As part of a health monitoring programme at Mulungushi International Conference Centre, Lusaka, Zambia, a coprological examination was undertaken to investigate the helminthological status of captive impala (Aepyceros melampus). Of 39 fresh coprological samples, 46% contained F. gigantica. The source of infection was identified to be a fountain within the grounds of the conference centre. Lymnaea natalensis, collected from the study site, were induced to shed cercariae, and were thus confirmed as the snail intermediate host. In managing this disease, triclabendazole at 6 mg/kg was administered together with the feed bait. Water from the pond was drained; vegetation within it cleared and the pond allowed to dry for 1 week before water was replaced. Three weeks post-treatment, faecal examination revealed that the Fasciola had been cleared. To the best of our knowledge, this is a first case of Fasciola spp. infection reported in captive wild impala in Zambia and provides evidence that triclabendazole may be delivered to free-ranging antelope using medicated bait.
Distribution and status of the African forest buffalo Syncerus caffer nanus in south-eastern Nigeria
Authors: Eniang Edem A. , Ebin Clement O. , Nchor Ayuk A. , Agba Gabriel I. , Gideon Idorenyin K. , Amori Giovanni , Luiselli Luca
Published: May 2016

Although not categorized as threatened on the IUCN Red List, the African forest buffalo Syncerus caffer nanus is declining across its range. In Nigeria its distribution, abundance and status are virtually unknown. We conducted interviews with experienced hunters, and field surveys (linear and recce transects), to study the buffalo's distribution and ecology in the montane forests of Cross River State. General linear modelling indicated that the number of individuals varied significantly across survey areas and habitat types but not with the survey period, and there was no study area × study period interaction. Buffalo were found most commonly in mature and secondary forests. Given the species’ scattered distribution, fragmentation of its habitat, and the relatively low numbers observed, Nigerian populations require a separate, regional categorization on the IUCN Red List.
Dynamic cerebral reorganization in the pathophysiology of schizophrenia: a MRI-derived cortical thickness study
Authors: Guo S. , Palaniyappan L. , Liddle P. F. , Feng J.
Published: May 2016

Background

A structural neuroanatomical change indicating a reduction in brain tissue is a notable feature of schizophrenia. Several pathophysiological processes such as aberrant cortical maturation, progressive tissue loss and compensatory tissue increase could contribute to the structural changes seen in schizophrenia.

Method

We studied cortical thickness using surface-based morphometry in 98 clinically stable patients with schizophrenia and 83 controls. Using a pattern classification approach, we studied whether the features that discriminate patients from controls vary across the different stages of the illness. Using a covariance analysis, we also investigated if concurrentincreasesaccompanydecreasesin cortical thickness.

Results

Very high levels of accuracy (96.3%), specificity (98.8%) and sensitivity (88%) were noted when classifying patients with <2 years of illness from controls. Within the patient group, reduced thickness was consistently accompanied by increased thickness in distributed brain regions. A pattern of cortical amelioration or normalization (i.e. reduced deviation from controls) was noted with increasing illness duration. While temporo-limbic and fronto-parietal regions showed reduced thickness, the occipital cortex showed increased thickness, especially in those with a long-standing illness.

Conclusion

A compensatory remodelling process might contribute to the cortical thickness variations in different stages of schizophrenia. Subtle cerebral reorganization reflecting the inherent plasticity of brain may occur concomitantly with processes contributing to tissue reduction in adult patients with schizophrenia.
Why clams have the shape they have: an experimental analysis of burrowing
Authors: Stanley Steven M.
Published: May 2016

The prosogyrous shape and flattened lunule of a typical clam shell form a blunt anterior, the function of which is related to the forward-and-back rocking motion of the shell in burrowing. Analysis of movies has revealed that each rocking motion of a morphologically typical clam, Mercenaria mercenaria (Linné), involves purely rotational movement, with no translational component. The clam is able to burrow by “walking” its way downward only because the axis of backward rotation lies to the anterior of the axis of forward rotation. Experiments with burrowing robots show that the blunt anterior serves to shift the axis of backward rotation anteriorly, thus aiding in downward progress. The prosogyrous condition and the rotational mechanism of burrowing are fundamental adaptations of burrowing clams and were apparently present in the ancestral bivalves of the Cambrian.
Chromosomal Mechanisms in the Evolution of Artiodactyls
Authors: Todd Neil B.
Published: May 2016

Evidence is presented that primitive artiodactyls had a diploid number of 14. The higher diploid numbers of most living artiodactyls are interpreted as resulting from karyotypic fissioning at the times of past adaptive radiations. The fossil record appears to support this contention.

An evolutionary sequence of unusual X chromosome transformations has been deduced from the differences that exist among extant species. From these, and from interrelationships of karyotypes, certain phylogenetic revisions are suggested.
Ecological succession as an aspect of structure in fossil communities
Authors: Walker Kenneth R. , Alberstadt Leonard P.
Published: May 2016

Succession involves changes in a community through time, whether internally or externally controlled. As succession progresses, niche specialization, species diversity (variety and equitability), complexity of food chains, and pattern diversity increase; net production and species growth rate decrease. We apply the succession concept to three types of ancient community sequences: 1) fossil reefs (Ordovician—Cretaceous in age), 2) short-term successions occurring through thin stratigraphic intervals, and 3) long-term successions occurring through thicker stratigraphic intervals. Ancient reefs show four vertical zones: (1) a basal stabilization zone (autogenic), 2) the overlying colonization zone (autogenic, pioneer stage), 3) the diversification zone, the bulk of most reefs (diversification culminating in climax), and 4) the uppermost domination zone. The first three zones represent autogenic succession but the final stage may involve allogenic succession. Short-term succession usually occurs where periodic allogenic catastrophes wipe out the community which is rebuilt through autogenic succession. Opportunistic pioneer species are important and in our examples (Ordovician, Silurian, and Cretaceous) are species which pave soft substrata. Paleozoic strophomenid brachiopods filled this role, and inoceramid pelecypods served the function in the Mesozoic. The succession which begins with opportunists progresses to a climax community of equilibrists. Repetition of catastrophe-succession couplets produces a cyclic stratigraphic record. Long-term successions are recorded in thicker stratigraphic sequences, and are of two types: 1) autogenic succession in unchanging physical environments and 2) allogenic succession in changing physical environments. Our examples of these are from the Devonian Haragan-Bois D'Arc formations of Oklahoma and the Lime Creek Formation of Iowa. This type of succession represents a temporal-spatial mosaic. The Haragan data (unchanging environments) indicate characteristic, intergrading, and ubiquitous species in the brachiopod communities. Most ubiquitous species in the pioneer community were eurytopic opportunists. The Lime Creek data allows testing of the prediction that environmental changes cause regression to an earlier succession stage. The brachiopod communities after environmental changes have more ubiquitous and intergrading eurytopic species. These represent an earlier stage in the succession.
Stratigraphic biases in the analysis of taxonomic survivorship
Authors: Sepkoski J. John
Published: May 2016

Taxonomic survivorship curves may reflect stratigraphic as well as biologic factors. The approximately lognormal distribution of lengths of Phanerozoic time intervals produces an error in the estimation of taxonomic durations that is also lognormally distributed. As demonstrated by several simulated examples, this error may cause concave taxonomic survivorship curves to appear linear, especially if the maximum durations involved are relatively short. The error of estimation also makes highly concave taxonomic survivorship curves virtually unrecognizable. Incomplete sampling of the fossil record, on the other hand, may not be a serious problem in survivorship analysis. Simulated paleontological sampling employing a simple model suggests that survivorship curves tend to retain their original shapes even when as few as 20% of the taxa have been discovered. However, concave taxonomic survivorship curves tend to lose their concavity as efficiency of sampling declines.
Some comments on Van Valen's law of extinction
Authors: Salthe Stanley N.
Published: May 2016

The linear survivorship curve of Van Valen's Law can be generated from stochastic loss of lineages, given a decreasing sample size within an adaptive zone. No special biological explanation is required or called for in order to explain “linearity” in taxonomic survivorship curves.
The role of phyletic change in the evolution ofPseudocubus vema(Radiolaria)
Authors: Kellogg Davida E.
Published: May 2016

While the importance of allopatric speciation in the fossil record has long been underestimated, phyletic change within single unbranching lineages also occurs. The 50% increase in thoracic width observed in the radiolarian speciesPseudocubus vemafrom an Antarctic deep-sea core is a clear example of a long-term phyletic trend in a continuous fossil sequence. Phyletic change inP. vemaoccurred at varying rates, but changes in the morphologic rate of evolution do not correspond to any obvious breaks in the fossil record such as would be indicated by missing segments of the core's magnetic stratigraphy. Variation in thoracic width, as measured by the coefficient of variation, does not depend on the morphologic rate of evolution, proportional rate of evolution, nor the amount of time required for the width to change by one standard deviation, so much as it depends on whether change was accomplished by addition or removal of extreme phenotypes to or from the population.
Form and function in a Recent free living brachiopod Magadina cumingi
Authors: Richardson Joyce R. , Watson Jeanette E.
Published: May 2016

Magadina cumingi (Davidson) lives in an environment of high current energy and mobile sediments using the pedicle as an elevating device. This previously undescribed substrate relationship is reflected in pedicle form, the muscular system, distribution of secondary thickening and characters of the beak. The cardinalia provide the evidence of key differences in the muscular system from an attached species Magellania australis (Quoy and Gaimard) and a free lying species with atrophied pedicle Neothyris lenticularis (Deshayes). The possession of an open foramen in articulate brachiopods is not synonymous with permanent attachment.
Strength of pedicle attachment in articulate brachiopods: ecologic and paleoecologic significance
Authors: Thayer Charles W.
Published: May 2016

It has been claimed that articulate brachiopods are excluded from turbulent environments by the weakness of their pedicle attachment. The evidence does not support this conclusion. Experiments on the force required to remove brachiopods from their substrate show that removal force often equals that of bivalve mussels from turbulent environments having the same valve area. Intertidal brachiopods probably occupy cryptic habitats to avoid desiccation, not to avoid waves. Subtidal brachiopods are abundant in tidal currents of 4 knots (2 m/sec) where they are usually attached in exposed positions.

Although paleoecologists often assume that the size of the pedicle foramen is directly proportional to attachment strength, the relationship is not precise. Experimental detachment of brachiopods resulted from failure of the substrate, pedicle-substrate attachment, pedicle, or pedicle adjustor muscles, in approximately equal frequencies. At the same attachment strength, the foramen in Terebratalia is four times the size of the foramen in Hemithiris.

Although subapical foramina are supposedly unable to enlarge as the brachiopod grows, the subapical foramen of Hemithiris appears to do so.
Stationary patterns and their selection mechanism of urban crime models with heterogeneous near-repeat victimization effect
Authors: GU YU , WANG QI , YI GUANGZENG
Published: May 2016

In this paper, we study two PDEs that generalize the urban crime model proposed by Short et al. (2008 Math. Models Methods Appl. Sci.18, 1249–1267). Our modifications are made under assumption of the spatial heterogeneity of both the near-repeat victimization effect and the dispersal strategy of criminal agents. We investigate pattern formations in the reaction–advection–diffusion systems with non-linear diffusion over multi-dimensional bounded domains subject to homogeneous Neumann boundary conditions. It is shown that the positive homogeneous steady state loses its stability as the intrinsic near-repeat victimization rate ε decreases and spatially inhomogeneous steady states emerge through bifurcation. Moreover, we find the wavemode selection mechanism through rigorous stability analysis of these non-trivial spatial patterns, which shows that the only stable pattern must have a wavenumber that maximizes the bifurcation value. Based on this wavemode selection mechanism, we will be able to predict the formation of stable aggregates of the house attractiveness and criminal population density, at least when the diffusion rate ε is around the principal bifurcation value. Our theoretical results also suggest that large domains support more stable aggregates than small domains. Finally, we perform extensive numerical simulations over 1D intervals and 2D squares to illustrate and verify our theoretical findings. Our numerics also demonstrate the formation of other interesting patterns in these models such as the merging of two interior spikes and the emerging of new spikes, etc. These non-trivial solutions can model the well-observed phenomenon of aggregation in urban criminal activities.
Neonatal management and outcomes of prenatally diagnosed CHDs
Authors: Bensemlali Myriam , Bajolle Fanny , Laux Daniela , Parisot Pauline , Ladouceur Magalie , Fermont Laurent , Lévy Marilyne , Le Bidois Jérôme , Raimondi Francesca , Ville Yves , Salomon Laurent J. , Boudjemline Younes , Bonnet Damien
Published: May 2016

Objectives

The aim of this study was to determine the probability of intervention at birth after prenatal diagnosis of CHD.

Methods

A 10-year retrospective study including all foetuses with a prenatally diagnosed CHD and those delivered in a tertiary-care cardiac centre between January, 2002 and December, 2011 was carried out. Patients were classified into eight groups according to the anticipated risk of neonatal intervention.

Results

The need for urgent intervention and/or PGE1 infusion within the first 48 hours of life was 47% (n=507/1080): 72% (n=248) for CHD at risk for a Rashkind procedure, 77% (n=72) for CHD with ductal-dependent pulmonary flow, 13% (n=22) for CHD with potentially ductal-dependent pulmonary flow, 94% (n=62) for CHD with ductal-dependent systemic flow, 29% (n=88) for CHD with potentially ductal-dependant systemic flow, 50% (n=4) for total anomalous pulmonary venous connection, and 17% (n=1) for CHD with atrio-ventricular block. In all, 34% of the patients received PGE1 infusion and 21.4% underwent urgent catheter-based or surgical interventions; 10% of patients without anticipated risk (n=10) underwent an early intervention; 6.7% (n=73) of the patients died; and 55% (n=589) had an intervention before discharge from hospital.

Conclusion

Half of the neonates with foetal CHD benefited from an urgent intervention or PGE1 infusion at birth. We recommend scheduled delivery and in utero transfer for transposition of the great arteries, double-outlet right ventricle with sub-pulmonary ventricular septal defect, total anomalous pulmonary venous connection, CHD with atrio-ventricular block with heart rate <50, all ductal-dependant lesions, and CHD with potentially ductal-dependant systemic flow.
Left ventricular end-diastolic dimension as a predictive factor of outcomes in children with acute myocarditis
Authors: Kim Geena , Ban Gil-Ho , Lee Hyoung-Doo , Sung Si-Chan , Kim Hyungtae , Choi Kwang-Ho
Published: May 2016

In this study, we sought predictors of mortality in children with acute myocarditis and of incomplete recovery in the survivor group. We classified our patients into three groups according to their outcomes at last follow-up: full recovery was classified as group I, incomplete recovery was classified as group II, and death was classified as group III. In total, 55 patients were enrolled in the study: 33 patients in group I, 11 patients in group II, and 11 patients in group III. The initial left ventricular fractional shortening – left ventricular fractional shortening – was significantly lower in group III (p=0.001), and the left ventricular end-diastolic dimension z score was higher in groups II and III compared with group I (p=0.000). A multivariate analysis showed that the left ventricular end-diastolic dimension z score (odds ratio (OR), 1.251; 95% confidence interval (CI), 1.004–1.559), extracorporeal membrane oxygenation (OR, 9.842; 95% CI, 1.044–92.764), and epinephrine infusion (OR, 18.552; 95% CI, 1.759–195.705) were significant predictors of mortality. The left ventricular end-diastolic dimension z score was the only factor that predicted incomplete recovery in the survivor group (OR, 1.360; 95% CI, 1.066–1.734; p=0.013). The receiver operating characteristic curve of the left ventricular end-diastolic dimension z score at admission showed a cut-off level of 3.01 for predicting mortality (95% CI, 0.714–0.948). In conclusion, a high left ventricular end-diastolic dimension z score on admission was a significant predictor of worse outcomes, both regarding mortality and incomplete recovery.
The value of right ventricular longitudinal strain in the evaluation of adult patients with repaired tetralogy of Fallot: a new tool for a contemporary challenge
Authors: Almeida-Morais Luís , Pereira-da-Silva Tiago , Branco Luísa , Timóteo Ana T. , Agapito Ana , de Sousa Lídia , Oliveira José A. , Thomas Boban , Jalles-Tavares Nuno , Soares Rui , Galrinho Ana , Cruz-Ferreira Rui
Published: May 2016

Objective

The role of right ventricular longitudinal strain for assessing patients with repaired tetralogy of Fallot is not fully understood. In this study, we aimed to evaluate its relation with other structural and functional parameters in these patients.

Methods

Patients followed-up in a grown-up CHD unit, assessed by transthoracic echocardiography, cardiac MRI, and treadmill exercise testing, were retrospectively evaluated. Right ventricular size and function and pulmonary regurgitation severity were assessed by echocardiography and MRI. Right ventricular longitudinal strain was evaluated in the four-chamber view using the standard semiautomatic method.

Results

In total, 42 patients were included (61% male, 32±8 years). The mean right ventricular longitudinal strain was −16.2±3.7%, and the right ventricular ejection fraction, measured by MRI, was 42.9±7.2%. Longitudinal strain showed linear correlation with tricuspid annular systolic excursion (r=−0.40) and right ventricular ejection fraction (r=−0.45) (all p<0.05), which in turn showed linear correlation with right ventricular fractional area change (r=0.50), pulmonary regurgitation colour length (r=0.35), right ventricular end-systolic volume (r=−0.60), and left ventricular ejection fraction (r=0.36) (all p<0.05). Longitudinal strain (β=−0.72, 95% confidence interval −1.41, −0.15) and left ventricular ejection fraction (β=0.39, 95% confidence interval 0.11, 0.67) were independently associated with right ventricular ejection fraction. The best threshold of longitudinal strain for predicting a right ventricular ejection fraction of <40% was −17.0%.

Conclusions

Right ventricular longitudinal strain is a powerful method for evaluating patients with tetralogy of Fallot. It correlated with echocardiographic right ventricular function parameters and was independently associated with right ventricular ejection fraction derived by MRI.
Gender difference in the association between food away-from-home consumption and body weight outcomes among Chinese adults
Authors: Du Wen-Wen , Zhang Bing , Wang Hui-Jun , Wang Zhi-Hong , Su Chang , Zhang Ji-Guo , Zhang Ji , Jia Xiao-Fang , Jiang Hong-Ru
Published: May 2016

Objective

The present study aimed to explore the associations between food away-from-home (FAFH) consumption and body weight outcomes among Chinese adults.

Design

FAFH was defined as food prepared at restaurants and the percentage of energy from FAFH was calculated. Measured BMI and waist circumference (WC) were used as body weight outcomes. Quantile regression models for BMI and WC were performed separately by gender.

Setting

Information on demographic, socio-economic, diet and health parameters at individual, household and community levels was collected in twelve provinces of China.

Subjects

A cross-sectional sample of 7738 non-pregnant individuals aged 18–60 years from the China Health and Nutrition Survey 2011 was analysed.

Results

For males, quantile regression models showed that percentage of energy from FAFH was associated with an increase in BMI of 0·01, 0·01, 0·01, 0·02, 0·02 and 0·03 kg/m2 at the 5th, 25th, 50th, 75th, 90th and 95th quantile, and an increase in WC of 0·04, 0·06, 0·06, 0·04, 0·06, 0·05 and 0·07 cm at the 5th, 10th, 25th, 50th, 75th, 90th and 95th quantile. For females, percentage of energy from FAFH was associated with 0·01, 0·01, 0·01 and 0·02 kg/m2 increase in BMI at the 10th, 25th, 90th and 95th quantile, and with 0·05, 0·04, 0·03 and 0·03 cm increase in WC at the 5th, 10th, 25th and 75th quantile.

Conclusions

Our findings suggest that FAFH consumption is relatively more important for BMI and WC among males rather than females in China. Public health initiatives are needed to encourage Chinese adults to make healthy food choices when eating out.
Quantifying Variability of Manual Annotation in Cryo-Electron Tomograms
Authors: Hecksel Corey W. , Darrow Michele C. , Dai Wei , Galaz-Montoya Jesús G. , Chin Jessica A. , Mitchell Patrick G. , Chen Shurui , Jakana Jemba , Schmid Michael F. , Chiu Wah
Published: May 2016

Although acknowledged to be variable and subjective, manual annotation of cryo-electron tomography data is commonly used to answer structural questions and to create a “ground truth” for evaluation of automated segmentation algorithms. Validation of such annotation is lacking, but is critical for understanding the reproducibility of manual annotations. Here, we used voxel-based similarity scores for a variety of specimens, ranging in complexity and segmented by several annotators, to quantify the variation among their annotations. In addition, we have identified procedures for merging annotations to reduce variability, thereby increasing the reliability of manual annotation. Based on our analyses, we find that it is necessary to combine multiple manual annotations to increase the confidence level for answering structural questions. We also make recommendations to guide algorithm development for automated annotation of features of interest.
Context and Media Frames: The Case of Liberia
Authors: Adams Melinda
Published: May 2016

There is a growing body of work examining gender stereotypes in media representations of female candidates, but much of this literature is based on analysis of media sources in developed countries, including the United States (Braden 1996; Jalalzai 2006; Kahn 1994, 1996; Smith 1997), Australia (Kittilson and Fridkin 2008), Canada (Kittilson and Fridkin 2008), France (Murray 2010b), and Germany (Wiliarty 2010). The increase in female presidential candidates and presidents in Latin America has encouraged research on media portrayals of women in Argentina, Chile, and Venezuela (Franceschet and Thomas 2010; Hinojosa 2010; Piscopo 2010; Thomas and Adams 2010). To date, however, there has been little research exploring media representations of female politicians in Africa. (Exceptions include Adams 2010; Anderson, Diabah, and hMensah 2011). A question that emerges is whether the gender stereotypes common in coverage in the United States, Europe, and Latin America are also prevalent in Africa.
Effect of the Theta-Beta Neurofeedback Protocol as a Function of Subtype in Children Diagnosed with Attention Deficit Hyperactivity Disorder
Authors: Duarte Hernández Elimelech , González Marqués Javier , Alvarado Jesús M.
Published: May 2016

Neurofeedback is a neuronal self-regulation technique that teaches people to modulate their brain frequencies using visual and auditory reinforcements presented on a computer screen. To assess the effect of neurofeedback training in children with ADHD as far as improved attention and impulse control, and analyze whether or not there are differences between the inattentive and hyperactive subtypes. Fifty children diagnosed with ADHD participated in the study: 14 comprised the control group, and 36 the experimental group (16 with the inattentive ADHD subtype, 20 with the hyperactive ADHD subtype). Attention and impulse control were assessed using the Integrated Visual Auditory CPT (IVA/CPT). Results indicated that the predominantly inattentive group showed significant differences on the Control Scale (p = .023, d = 1.31) and the Attention Scale (p < .01, d = 1.89) of the IVA/CPT; meanwhile the predominantly hyperactive group showed significant improvement on the Control Scale (p = .016, d = 1.21). The control group exhibited no significant differences on either of the two scales (p > .5). In terms of theta/beta ratio, no significant differences were detected (p = .10) between ADHD subtypes. The findings suggest that neurofeedback training using the theta/beta protocol was more effective in the predominantly inattentive subset of individuals with ADHD.
Ocean Floor Networks Capture Low-Frequency Earthquake Event
Authors: Nakano Masaru , Hori Takane , Araki Eiichiro , Takahashi Narumi , Kodaira Shuichi
Published: May 2016

Last August, stations on a newly deployed permanent ocean floor observation network recorded rarely seen, very low frequency signals from shallow earthquakes.
How on Earth to Decide Where on Mars to Land?
Authors: Wilson Claire
Published: May 2016

The Public Lecture at AGU's 2016 Fall Meeting will feature three experts—including one still in high school—to discuss landing site selection for the Mars 2020 rover.
A spin-liquid with pinch-line singularities on the pyrochlore lattice
Authors: Benton Owen , Jaubert L.D.C. , Yan Han , Shannon Nic
Published: May 2016

The mathematics of gauge theories lies behind many of the most profound advances in physics in the past 200 years, from Maxwell’s theory of electromagnetism to Einstein’s theory of general relativity. More recently it has become clear that gauge theories also emerge in condensed matter, a prime example being the spin-ice materials which host an emergent electromagnetic gauge field. In spin-ice, the underlying gauge structure is revealed by the presence of pinch-point singularities in neutron-scattering measurements. Here we report the discovery of a spin-liquid where the low-temperature physics is naturally described by the fluctuations of a tensor field with a continuous gauge freedom. This gauge structure underpins an unusual form of spin correlations, giving rise to pinch-line singularities: line-like analogues of the pinch points observed in spin-ice. Remarkably, these features may already have been observed in the pyrochlore material Tb2Ti2O7.
The machinery underlying malaria parasite virulence is conserved between rodent and human malaria parasites
Authors: De Niz Mariana , Ullrich Ann-Katrin , Heiber Arlett , Blancke Soares Alexandra , Pick Christian , Lyck Ruth , Keller Derya , Kaiser Gesine , Prado Monica , Flemming Sven , del Portillo Hernando , Janse Chris J. , Heussler Volker , Spielmann Tobias
Published: May 2016

Sequestration of red blood cells infected with the human malaria parasite Plasmodium falciparum in organs such as the brain is considered important for pathogenicity. A similar phenomenon has been observed in mouse models of malaria, using the rodent parasite Plasmodium berghei, but it is unclear whether the P. falciparum proteins known to be involved in this process are conserved in the rodent parasite. Here we identify the P. berghei orthologues of two such key factors of P. falciparum, SBP1 and MAHRP1. Red blood cells infected with P. berghei parasites lacking SBP1 or MAHRP1a fail to bind the endothelial receptor CD36 and show reduced sequestration and virulence in mice. Complementation of the mutant P. berghei parasites with the respective P. falciparum SBP1 and MAHRP1 orthologues restores sequestration and virulence. These findings reveal evolutionary conservation of the machinery underlying sequestration of divergent malaria parasites and support the notion that the P. berghei rodent model is an adequate tool for research on malaria virulence.
BDNF rescues BAF53b-dependent synaptic plasticity and cocaine-associated memory in the nucleus accumbens
Authors: White André O. , Kramár Enikö A. , López Alberto J. , Kwapis Janine L. , Doan John , Saldana David , Davatolhagh M. Felicia , Alaghband Yasaman , Blurton-Jones Mathew , Matheos Dina P. , Wood Marcelo A.
Published: May 2016

Recent evidence implicates epigenetic mechanisms in drug-associated memory processes. However, a possible role for one major epigenetic mechanism, nucleosome remodelling, in drug-associated memories remains largely unexplored. Here we examine mice with genetic manipulations targeting a neuron-specific nucleosome remodelling complex subunit, BAF53b. These mice display deficits in cocaine-associated memory that are more severe in BAF53b transgenic mice compared with BAF53b heterozygous mice. Similar to the memory deficits, theta-induced long-term potentiation (theta-LTP) in the nucleus accumbens (NAc) is significantly impaired in slices taken from BAF53b transgenic mice but not heterozygous mice. Further experiments indicate that theta-LTP in the NAc is dependent on TrkB receptor activation, and that BDNF rescues theta-LTP and cocaine-associated memory deficits in BAF53b transgenic mice. Together, these results suggest a role for BAF53b in NAc neuronal function required for cocaine-associated memories, and also that BDNF/TrkB activation in the NAc may overcome memory and plasticity deficits linked to BAF53b mutations.
Climatic and environmental aspects of the Mongol withdrawal from Hungary in 1242 CE
Authors: Büntgen Ulf , Di Cosmo Nicola
Published: May 2016

The Mongol invasion of Eastern Europe and especially its sudden withdrawal from Hungary in 1242 CE, has generated much speculation and an array of controversial theories. None of them, however, considered multifaceted environmental drivers and the coupled analysis of historical reports and natural archives. Here we investigate annually resolved, absolutely dated and spatially explicit paleoclimatic evidence between 1230 and 1250 CE. Documentary sources and tree-ring chronologies reveal warm and dry summers from 1238–1241, followed by cold and wet conditions in early-1242. Marshy terrain across the Hungarian plain most likely reduced pastureland and decreased mobility, as well as the military effectiveness of the Mongol cavalry, while despoliation and depopulation ostensibly contributed to widespread famine. These circumstances arguably contributed to the determination of the Mongols to abandon Hungary and return to Russia. While overcoming deterministic and reductionist arguments, our ‘environmental hypothesis’ demonstrates the importance of minor climatic fluctuations on major historical events.
Conservation of Terrestrial Vertebrates in a Global Hotspot of Karst Area in Southwestern China
Authors: Luo Zhenhua , Tang Songhua , Jiang Zhigang , Chen Jing , Fang Hongxia , Li Chunwang
Published: May 2016

The karst area of southwest China (KASC) is the largest piece of karst landscape on the earth and a global biodiversity hot-spot with high concentrations of endemic species. Although a number of nature reserves (NRs) have been established across the region, the representativeness of biodiversity of the NR system is still unknown. Based on comprehensive literature and field surveys and intensive consultations with zoologists and wildlife managers, we compiled distributions of 1,204 terrestrial vertebrate species and 271 NRs in KASC. We found Jinxiu, Mengla, Hekou and Jinghong have the richest amphibian species; Jinxiu has the highest species richness of reptiles; Jinghong, Menghai and Mengla have the largest numbers of avian species; whereas, Mengla, Longzhou and Ningming have the greatest mammalian diversity in the region. Gap analysis among NR system, species richness pattern and five biogeographic indicators found insufficient representation of the NR system on territorial vertebrate diversity. The conservation effectiveness in Guizhou Province was much lower than that in Guangxi and Yunnan Provinces. Under-representation and over-representation simultaneously occurred in many of the ecoregions, elevation classes, vegetation types, landcover categories and human disturbance intensity gradients. For conservation of terrestrial vertebrates in KASC, several suggestions were presented in this study.
Glucocorticoid receptor isoforms direct distinct mitochondrial programs to regulate ATP production
Authors: Morgan David J. , Poolman Toryn M. , Williamson Andrew J. K. , Wang Zichen , Clark Neil R. , Ma’ayan Avi , Whetton Anthony D. , Brass Andrew , Matthews Laura C. , Ray David W.
Published: May 2016

The glucocorticoid receptor (GR), a nuclear receptor and major drug target, has a highly conserved minor splice variant, GRγ, which differs by a single arginine within the DNA binding domain. GRγ, which comprises 10% of all GR transcripts, is constitutively expressed and tightly conserved through mammalian evolution, suggesting an important non-redundant role. However, to date no specific role for GRγ has been reported. We discovered significant differences in subcellular localisation, and nuclear-cytoplasmic shuttling in response to ligand. In addition the GRγ transcriptome and protein interactome was distinct, and with a gene ontology signal for mitochondrial regulation which was confirmed using Seahorse technology. We propose that evolutionary conservation of the single additional arginine in GRγ is driven by a distinct, non-redundant functional profile, including regulation of mitochondrial function.
Impact of Different Promoters on Episomal Vectors Harbouring Characteristic Motifs of Matrix Attachment Regions
Authors: Wang Xiao-Yin , Zhang Jun-He , Zhang Xi , Sun Qiu-Li , Zhao Chun-Peng , Wang Tian-Yun
Published: May 2016

We previously demonstrated that the characteristic sequence of matrix attachment regions (MARs) allows transgenes to be maintained episomally in CHO cells. In the present study, six commonly used promoters from human cytomegalovirus major immediate-early (CMV), simian vacuolating virus 40 (SV40), Rous sarcoma virus, Homo sapiens ubiquitin C, phosphoglycerate kinase and β-globin, respectively, were evaluated to determine their effects on transgene expression and stability in CHO cells stably transfected via the episomal vector harbouring characteristic MAR motifs. The CHO cells were transfected with vectors and then screened using G418, after which the stably transfected cells were split into two and further cultured either in the presence or absence of G418. Of the six promoters, the CMV promoter yielded the highest transgene expression levels and the highest transfection efficiency, whereas the SV40 promoter maintained transgene expression more stably during long-term culture than the other promoters did. The CMV and SV40 promoter-containing vectors were furthermore episomally maintained and conferred sustained eGFP expression in the cells even under nonselective conditions. On the basis of these findings, we conclude that the CMV promoter performs best in terms of yielding both high expression levels and high levels of stability using this episomal vector system.
Systematic analysis of mutation distribution in three dimensional protein structures identifies cancer driver genes
Authors: Fujimoto Akihiro , Okada Yukinori , Boroevich Keith A. , Tsunoda Tatsuhiko , Taniguchi Hiroaki , Nakagawa Hidewaki
Published: May 2016

Protein tertiary structure determines molecular function, interaction and stability of the protein, therefore distribution of mutation in the tertiary structure can facilitate the identification of new driver genes in cancer. To analyze mutation distribution in protein tertiary structures, we applied a novel three dimensional permutation test to the mutation positions. We analyzed somatic mutation datasets of 21 types of cancers obtained from exome sequencing conducted by the TCGA project. Of the 3,622 genes that had ≥3 mutations in the regions with tertiary structure data, 106 genes showed significant skew in mutation distribution. Known tumor suppressors and oncogenes were significantly enriched in these identified cancer gene sets. Physical distances between mutations in known oncogenes were significantly smaller than those of tumor suppressors. Twenty-three genes were detected in multiple cancers. Candidate genes with significant skew of the 3D mutation distribution included kinases (MAPK1,EPHA5,ERBB3andERBB4), an apoptosis related gene (APP), an RNA splicing factor (SF1), a miRNA processing factor (DICER1), an E3 ubiquitin ligase (CUL1) and transcription factors (KLF5andEEF1B2). Our study suggests that systematic analysis of mutation distribution in the tertiary protein structure can help identify cancer driver genes.
Up-regulated extracellular matrix components and inflammatory chemokines may impair the regeneration of cholestatic liver
Authors: Zhang Shuai , Li Tao-Sheng , Soyama Akihiko , Tanaka Takayuki , Yan Chen , Sakai Yusuke , Hidaka Masaaki , Kinoshita Ayaka , Natsuda Koji , Fujii Mio , Kugiyama Tota , Baimakhanov Zhassulan , Kuroki Tamotsu , Gu Weili , Eguchi Susumu
Published: May 2016

Although the healthy liver is known to have high regenerative potential, poor liver regeneration under pathological conditions remains a substantial problem. We investigated the key molecules that impair the regeneration of cholestatic liver. C57BL/6 mice were randomly subjected to partial hepatectomy and bile duct ligation (PH+BDL group, n = 16), partial hepatectomy only (PH group, n = 16), or sham operation (Sham group, n = 16). The liver sizes and histological findings were similar in the PH and sham groups 14 days after operation. However, compared with those in the sham group, the livers in mice in the PH+BDL group had a smaller size, a lower cell proliferative activity and more fibrotic tissue 14 days after the operation, suggesting the insufficient regeneration of the cholestatic liver. Pathway-focused array analysis showed that many genes were up- or down-regulated over 1.5-fold in both PH+BDL and PH groups at 1, 3, 7 and 14 days after treatment. Interestingly, more genes that were functionally related to the extracellular matrix and inflammatory chemokines were found in the PH+BDL group than in the PH group at 7 and 14 days after treatment. Our data suggest that up-regulated extracellular matrix components and inflammatory chemokines may impair the regeneration of cholestatic liver.
The significance of droughts for hyporheic dwellers: evidence from freshwater crayfish
Authors: Kouba Antonín , Tíkal Jan , Císař Petr , Veselý Lukáš , Fořt Martin , Příborský Josef , Patoka Jiří , Buřič Miloš
Published: May 2016

Freshwater biodiversity is globally threatened by various factors while severe weather events like long-term droughts may be substantially devastating. In order to remain in contact with the water or stay in a sufficiently humid environment at drying localities, the ability to withstand desiccation by dwelling in the hyporheic zone, particularly through vertical burrowing is crucial. We assessed the ability of three European native and five non-native crayfish as models to survive and construct vertical burrows in a humid sandy-clayey substrate under a simulated one-week drought. Three native species (Astacus astacus, A. leptodactylus and Austropotamobius torrentium) suffered extensive mortalities. Survival of non-native species was substantially higher while all specimens of Cherax destructor and Procambarus clarkii survived. The native species and Pacifastacus leniusculus exhibited no ability to construct vertical burrows. Procambarus fallax f. virginalis and P. clarkii constructed bigger and deeper burrows than C. destructor and Orconectes limosus. In the context of predicted weather fluctuations, the ability to withstand desiccation through constructing vertical burrows into the hyporheic zone under drought conditions might play a significant role in the success of particular crayfish species, as well as a wide range of further hyporheic-dwelling aquatic organisms in general.