bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the use of POLY4 for the improvement of productivity, peanut quality, and soil properties in Southern India
Authors: Gopinath Kodigal A. , Rajanna Gandhamanagenahalli A. , Visha Kumari Venugopalan , Singh Vinod Kumar , Ajay B. C. , Awasthi Neeraj K. , Mishra Vipin , Sukumaran Suvana , Venkatesh Govindarajan , Rajkumar Bollam
Published: October 2024

Polyhalite-based POLY4, a multi-nutrient source containing potassium, calcium, magnesium, and sulphur, is increasingly recognised for its potential to improve crop yields and soil health in agricultural systems. It is also been considered as a feasible approach for addressing the deficiency in potassium, calcium, and sulphur within a single application source. The present study aimed to investigate the impact of polyhalite-based POLY4 application, either as a complete or partial substitute for traditional potassium fertiliser and gypsum supplement, on the improvement of peanut (Arachis hypogaea) growth and soil quality. An extensive field study was conducted from 2021 and 2022, employing ten distinct nutrient management treatments with three replications in a randomised complete block design. The findings of the study indicated that the application of polyhalite (POLY4) in conjunction with only NP fertilisers resulted in a higher yield advantage (approximately 150–200 kg ha-1) than in plots treated with NPK + gypsum (at 500 kg ha-1) and control plots. The application of polyhalite-based fertiliser (POLY4) at a rate that was 100% equivalent to K along with NP fertilisers resulted in a significant increase in pod yield (5.3–12.8%) over NPK + gypsum and control plots. Thus, the increased crop yield led to an increase in gross returns of 4.88% and in net returns of 4.28% with the application of POLY4 (100%) + NP fertilisers over other treatments. Likewise, variable rates of conventional fertilisers along with POLY4 (100% recommended) + NP + gypsum at 310 kg/ha significantly increased the linoleic acid content (38.5%), oleic acid content, and oil content (48.1%) by reducing palmitic acid (11.96%) content in the groundnut seed. Interestingly, POLY4 use at the 50% recommended rate also resulted in yields that were comparable with those obtained with 100% NPK. Therefore, applying POLY4, a polyhalite fertiliser, in either a 100% or 50% equivalent of essential K can be an effective way of increasing the production of peanut crops and promoting agricultural sustainability.
Weight-control compensatory behaviors patterns and correlates: a scoping review
Authors: Yuan Trista Yue , Bouzari Narges , Bains Andy , Cohen Tamara R. , Kakinami Lisa
Published: October 2024

Objective

Weight-control compensatory behaviors appear to be a commonly utilized strategy for health management. Individuals engaging in such behaviors believe that the negative consequences from unhealthy behaviors will be neutralized by the positive consequences of healthy behaviors. Existing research has not reached a consensus on whether such behaviors are beneficial to health. This review aims to (1) summarize the patterns of weight-control compensatory health behaviors in different populations, (2) highlight correlates, predictors, and consequences of compensatory health behaviors, and (3) identify gaps for future research.

Method

This review identified existing literature using online databases, CINAHL and PubMed. Primary research articles published after 2000 with non-clinical participants of 12 years or older who engaged in compensatory behaviors for weight control purposes were selected. Descriptive statistics were extracted from 35 studies.

Results

Different patterns for weight-control compensatory behaviors emerged between the female and male sexes. Meanwhile, no clear association of such behaviors was found across weight status. Studies reviewed also highlighted three main areas of compensatory behaviors for weight management, namely dietary behaviors, physical activity, and alcohol consumption. Weight-control compensatory behaviors had significant negative correlations with mental health indicators, such as psychosocial functioning, emotional differentiation ability, and body esteem.

Conclusion

Weight-control compensatory behaviors may be a widely used weight management strategy and can be presented in diverse ways. Although believed to be promoting health, such behaviors appear to be associated with poor psychological well-being. This emerging topic warrants more in-depth investigation to establish the direction of causation. Future research may investigate the relationship between weight-control compensatory behaviors and various aspects of health over longer time periods, examine the engagement of multiple weight-control compensatory behaviors, and focus on high-risk populations.
Playing-related musculoskeletal disorders among Chinese conservatoire piano students: prevalence, risk factors and preventive interventions
Authors: Zhao Luqian , Wang Yafei , Zhang Zheren
Published: October 2024

Introduction

Both professional musicians and conservatoire students are at significant risk of developing playing-related musculoskeletal disorders (PRMDs) during their career life. With the growing number of students pursuing a conservatory degree and graduating from music conservatory in China, the aims of this study were: (1) to identify the nature of PRMD and explore the prevalence of PRMD in Chinese conservatoire students; (2) to determine the relevant risk factors with the presence of PRMD among Chinese conservatoire students; and (3) to suggest preventive interventions for young pianists at their early career stage.

Methods

A self-reported online survey study was conducted among 363 Chinese conservatoire students who majored in piano performance.

Results

Of all respondents, 82.6% reported having had at least one PRMD. The wrist was proved to be the most affected body site, followed by the shoulder, finger and arm. Respondents who experienced PRMD reported “pain,” “fatigue,” and “stiffness” as the most frequent symptoms. The main risk factors associated with PRMD included gender, years of playing experiences, practice hours, warm-up habits before practice, and break-taking during practice.

Discussion

Female students, those with longer year of playing experience, those who practice longer hours, those who do not warm up before practice, and those who do not take breaks during practice were found to have more PRMD symptoms and higher level of severity. This study highlights the need to increase awareness of PRMD among conservatoire students and to understand the occurrence of PRMD; it is helpful for young pianists to prevent severe musculoskeletal disorders and implement preventive measures at early career stages. Further studies are suggested to follow up on music students who have had at least one PRMD at different stages of professional musical training.
Generation and discrimination of autism MRI images based on autoencoder
Authors: Shi Yuxin , Gong Yongli , Guan Yurong , Tang Jiawei
Published: October 2024

This study aims to explore an autoencoder-based method for generating brain MRI images of patients with Autism Spectrum Disorder (ASD) and non-ASD individuals, and to discriminate ASD based on the generated images. Initially, we introduce the research background of ASD and related work, as well as the application of deep learning in the field of medical imaging. Subsequently, we detail the architecture and training process of the proposed autoencoder model, and present the results of generating MRI images for ASD and non-ASD patients. Following this, we designed an ASD classifier based on the generated images and elucidated its structure and training methods. Finally, through analysis and discussion of experimental results, we validated the effectiveness of the proposed method and explored future research directions and potential clinical applications. This research offers new insights and methodologies for addressing challenges in ASD studies using deep learning technology, potentially contributing to the automated diagnosis and research of ASD.
Hepatotoxicity of antipsychotics: an exploratory pharmacoepidemiologic and pharmacodynamic study integrating FAERS data and in vitro receptor-binding affinities
Authors: Zeiss René , Schönfeldt-Lecuona Carlos , Connemann Bernhard J. , Hafner Susanne , Gahr Maximilian
Published: October 2024

Introduction

Antipsychotic psychopharmacotherapy is associated with the risk of drug-induced liver injury (DILI). However, understanding specific risk factors remains challenging due to limited data. This study investigates the relationship between receptor binding affinities and occupancies of antipsychotics and their associated hepatotoxic risks.

Methods

A disproportionality analysis with calculation of the Reporting Odds Ratio (ROR) and the Information Component (IC) was conducted using data from the FDA Adverse Event Reporting System (FAERS) to identify signals related to the Standardised MedDRA Query “drug-related hepatic disorders”, which served as a proxy for drug-induced hepatotoxicity. This was followed by a pharmacoepidemiologic-pharmacodynamic approach to investigate the relationship between the ROR and substance-related receptor binding affinities and occupancy, which was estimated based on in vitro receptor-binding profiles.

Results

Significant signals were identified for several antipsychotics, including chlorpromazine, loxapine, olanzapine, and quetiapine, with chlorpromazine and loxapine showing the highest RORs for DILI. Gender-specific analysis revealed a higher frequency of signals in female patients. Statistically significant negative correlations were identified between the ROR for drug-related hepatic disorders and the affinity for serotonin receptor 5-HT1A (r (17) = -0.68, p = 0.0012), while a positive correlation was observed for cholinergic receptors (r (17) = 0.46, p = 0.048). No significant correlations were found related to other receptors or drug properties.

Conclusion

Our findings suggest that the serotonin and probably the cholinergic system may play a role in the development of DILI related to antipsychotic medications. The identification of antipsychotics with a higher association with DILI, such as chlorpromazine, underscores the need for careful monitoring in clinical practice. However, our findings need further longitudinal studies to confirm causality. A better understanding of the associations may inform clinical decision-making, particularly in patients with an increased susceptibility to liver damage.
The impact of climate change-related disasters on mental health and epigenetics: a narrative review
Authors: Roe Emma Antonina , Khov Engluy , Kim Hannah , Demelo Beth , Zavitz Savannah , Cao-Lei Lei
Published: October 2024

Climate change has contributed to an increase in the frequency and intensity of natural disasters such as droughts, wildfires, hurricanes, and floods, leading to both immediate physical harm and long-term mental health consequences. Survivors often experience psychological distress, including anxiety, depression, and post-traumatic stress disorder (PTSD), as a result of these traumatic events. This narrative review explores the intersection of climate change-related disasters, mental health outcomes, and epigenetic modifications. Specifically, we summarize recent findings on how environmental stressors influence both mental health and epigenetic changes, such as DNA methylation. Emerging evidence suggests that epigenetic mechanisms, particularly DNA methylation, could mediate the effects of climate change-related stress on mental health, potentially contributing to the onset of mental disorders like depression, panic disorder, autism spectrum disorder, and attention deficit hyperactivity disorder. We also discuss other epigenetic mechanisms, such as histone modifications and non-coding RNAs, and emphasize the importance of longitudinal studies to capture the dynamic nature of epigenetic changes over time. Finally, we propose future research directions to deepen our understanding of the complex relationships between climate change-related disasters, mental health outcomes, and epigenetic mechanisms, which will pave the way for more effective mental health interventions and policy integration.
Building locally anchored implementation science capacity: the case of the adolescent HIV implementation science alliance-supported local iS alliances
Authors: Vorkoper Susan , Agot Kawango , Dow Dorothy E. , Mbizvo Michael , Mugo Cyrus , Sam-Agudu Nadia A. , Semitala Fred C. , Zanoni Brian C. , Sturke Rachel
Published: October 2024

Background

The Fogarty International Center-led Adolescent HIV Implementation Science Alliance (AHISA) supports region-/country-specific implementation science (IS) alliances that build collaborations between research, policy, and program partners that respond to local implementation challenges. AHISA supported the development of seven locally-led IS alliances: five country-specific (i.e., Kenya, South Africa, Tanzania, Uganda, and Zambia), one in Central and West Africa, and one with youth researchers. This article outlines the aims, activities, and outcomes of local alliances, demonstrating how they enhance sustainable IS activities to address local challenges.

Methods

We conducted a desk review of each alliance's funding applications, reports, and data from the initial findings of a larger AHISA evaluation. The review analyzes common approaches, highlights their local relevance, and summarizes initial outcomes.

Results

The local alliances have a common goal: to expand implementation of successful interventions to improve adolescent HIV. We identified four overarching themes across the local alliances’ activities: capacity building, priority setting, stakeholder engagement, and knowledge dissemination. Research capacity building activities include long-term mentorship between junior and senior researchers and short-term training for non-research partners. Setting priorities with members identifies local research needs and streamlines activities. Alliances incorporate substantial engagement between partners, particularly youth, who may serve as leaders and co-create activities. Dissemination shares activities and results broadly.

Conclusion

Local IS alliances play a key role in building sustainable IS learning and collaboration platforms, enabling improved uptake of evidence into policy and programs, increased IS research capacity, and shared approaches to addressing implementation challenges.
A functional trait-based assessment of urban street tree selection for Ethiopia
Authors: Tamene Gebretsadik , Sjöman Henrik , Sang Åsa Ode , Yeshitla Kumelachew , Belay Birhanu , Tesfaye Ergua , Levinsson Anna
Published: October 2024

Urbanization in Ethiopia is rapidly reducing green space and biodiversity, and placing ecological stress on trees. Urban street trees (UST) can provide multiple ecosystem services, but need to cope with challenging street ecology and future climate risks. However, UST selection in Ethiopia is poor, and conventional trials and modern breeding are costly and time consuming to apply in Ethiopia. The main objective of the study was to identify potential UST species for two Ethiopian cities; Addis Ababa, and Arba Minch, by studying interspecific differences in functional traits. For the purpose, we created a database with list of 120 candidate UST gathered from multi-stakeholder workshop, literature review, and senior experts survey, and their ecological with their phenological and morphological features. Then we filtered 25 potential UST through framework analysis considering eco-morphological features of the trees. For the 25 species, we measured three types of plant functional traits: conservative traits (leaf mass per area and leaf dry matter content), acquisitive traits (leaf area, specific leaf area and leaf water content) and drought tolerance traits (leaf turgor pressure loss point, and leaf succulence index). Our results showed a strong trade-off between conservative and acquisitive traits, particularly between leaf dry matter content (LDMC) and specific leaf area (SLA) (r = −0.71), consistent with plant economic spectrum theory. Using PCA analysis, we identified three groups of potential USTs (Type I, II and III) that exhibit different adaptation strategies, consistent with Grime’s trait-based classification of universal plant adaptation strategies (CSR). Compared to species with high acquisitive traits (Type II and Type III), Type I species have high conservative and drought-tolerant traits shows better adaptation to harsh road environments, whereas Type II and Type III species may be more advantageous for urban parks and other resource-rich components of urban ecology. We also found significant variation in conservative, acquisitive and drought-tolerant traits among different species, indicating their different adaptation strategies. Our research advances the knowledge of plant adaptation in urban environments and provides a useful method for UST selection.
Physically-based modelling of UK river flows under climate change
Authors: Smith Ben A. , Birkinshaw Stephen J. , Lewis Elizabeth , McGrady Eleyna , Sayers Paul
Published: October 2024

This study presents the model setup and results from the first calibrated, physically-based, spatially-distributed hydrological modelling of combined land cover and climate change impacts on a large sample of UK river catchments. The SHETRAN hydrological model was automatically calibrated for 698 UK catchments then driven by the 12 regional climate model projections from UKCP18, combined with urban development and natural flood management scenarios. The automatic calibration of SHETRAN produces a median Nash-Sutcliffe efficiency value of 0.82 with 581 catchments having a value greater than 0.7. 24 summary metrics were calculated to capture changes to important aspects of the flow regime. The UKCP18 realisations in SHETRAN indicate that a warming climate will cause river flows, on average, to decrease. These decreases are simulated to be greatest in the south and east of the UK, with droughts becoming longer and more severe. While high flows also decrease on average, an increased number of extremes are exhibited, implying a greater number of extreme flood events in the future, particularly in the north and west of the UK. In the urban development scenarios, for flood events there is an increase in flow with the increased urbanization, with the 1 in 3-year peak flow event showing the greatest increase. The natural flood management scenarios consider the effect of increasing woodland and adding surface water storage ponds. The inclusion of these features produces a complex response but overall, the modelling shows a reduction in low, median, and high flows, although the more extreme the flow event the smaller the percentage change in flow. Simulated timeseries and summary metric datasets are freely available on the CEDA archive.
Construction of a blockchain based cold chain logistics information platform for Gannan navel oranges to enhance transparency and efficiency
Authors: Xu Xifeng , Yuen Choon Wah , Koting Suhana Binti , Musa Siti Nurmaya Binti
Published: October 2024

As a high-value agricultural product, the cold chain logistics management of Gannan navel oranges has global significance. Especially in Africa and Europe, food safety and supply chain quality are critical issues. However, the existing Gannan navel orange logistics information management system relies on traditional databases and information systems, with insufficient data sharing and transparency, affecting the overall coordination of the supply chain and customer satisfaction. In addition, the traditional system is vulnerable to hacker attacks and malicious tampering of data by insiders, resulting in serious economic losses and reputation damage. So the blockchain platform constructed in this study improves the transparency and traceability of logistics data through the SMART-PBFT algorithm of the alliance chain, which optimizes the logistics management process, improves the quality of logistics services, and reduces operating costs. It improves the logistics efficiency and data transparency of local agricultural products, and also provides a demonstrative case for the cold chain management of other high-value agricultural products around the world, with broad application potential and reference value.
Microbiology of periprosthetic infections following implant-based breast reconstruction surgery: a multicentric retrospective study
Authors: Lisa Andrea Vittorio Emanuele , Zeneli Flavia , Mazzucco Martina , Barbieri Benedetta , Rietjens Mario , Lissidini Germana , Vinci Valeriano , Bartoletti Michele , Belati Alessandra , Bavaro Davide
Published: October 2024

Introduction

Implant-based breast reconstruction (IBR) is the predominant breast reconstruction technique post-mastectomy, with bacterial infections being a significant complication affecting patient recovery and quality of life. The following study aimed to determine the microbiological features of the causative agents responsible for breast implant infections, with more attention paid to the comparative analysis of Gram-positive and Gram-negative bacteria and their presentation.

Methods

We conducted a retrospective analysis of 214 patients who presented with periprosthetic infection and underwent implant removal following implant-based breast reconstruction at Humanitas Research Hospital and Istituto Europeo di Oncologia between January 2018 and March 2024.

Results

The study revealed that Gram-positive bacteria were more prevalent, with Staphylococcus species, particularly Staphylococcus aureus, being the most isolated pathogen in both institutions (∼39.96%). In contrast, Gram-negative bacteria were less frequent, with a higher proportion of these pathogens being multi-resistant strains. A significant difference was observed (p = 0.007), indicating that individuals with normal BMI have a higher prevalence of Gram-positive infections (88.46%), whereas obese and overweight patients had higher proportions of Gram-negative infections (23.53% and 28.89%, respectively). In addition, smoking status was also significantly associated with pathogen distribution (p = 0.032), with active and past smokers being related to higher percentages of polymicrobial infections. Furthermore, positive prophylactic MSSA/MRSA swabs were significantly more associated with Staphylococcus aureus infections compared to those with negative results (p = <0.001).

Conclusions

Gram-positive bacteria, especially Staphylococcus species, dominate the microbiological landscape of implant-based breast reconstruction (IBR) infections. Our findings provide insights into this critical issue, facilitating a more precise choice of empiric antibiotic treatment and prevention strategies. This analysis underscores the necessity for prophylactic protocols and therapeutic approaches tailored to the predominant bacterial groups. Further research is needed to explore long-term trends and resistance mechanisms to improve patient management.
ESOT Roadmap for Advanced Therapy Medicinal Products in Transplantation: Navigating Regulatory Challenges to Enhance Access and Care
Authors: Berishvili Ekaterine , Piemonti Lorenzo , de Koning Eelco J. P. , Lindstedt Sandra , Scholz Hanne , Scott William E. , Auxenfans Celine , Johnson Paul , Martin Dominique E. , Gunther Penilla , Mey Devi , Potena Luciano , Thaunat Olivier
Published: October 2024

The field of organ transplantation is experiencing a transformative shift with the rise of Advanced Therapy Medicinal Products (ATMPs), which include gene therapies, somatic cell therapies, and tissue-engineered products. These therapies offer new, potentially curative treatments for longstanding medical challenges, impacting numerous patients. However, their adoption is hindered by complex regulatory frameworks, high production costs, and inconsistent access across Europe. The ESOT ATMP Task Force’s position paper analyzes these challenges from research to clinical application, advocating for a coordinated strategy to position Europe as a leader in ATMP development. It proposes specific actions such as streamlining regulatory pathways to accelerate approvals, boosting funding for ATMP research, and creating specialized facilities for development and implementation. The paper also highlights the critical roles of patient engagement and real-world evidence in optimizing clinical and regulatory practices.
Intelligent Grinding System for Medium/Thick Plate Welding Seams in Construction Machinery Using 3D Laser Measurement and Deep Learning
Authors: Liu Qifeng , Zheng Rencheng , Li Pengchao , Liu Chao , Mi Deyuan , Wang Jian , Xie Wenli
Published: October 2024

With the rapid development of the construction machinery industry, thick plate welds are increasingly needing efficient, accurate, and intelligent processing. This study proposes an intelligent grinding system using 3D line laser measurement and deep learning algorithms to solve the problems of inefficiency and inaccuracy existing in traditional weld grinding methods. This study makes use of 3D line laser measurement technology and deep learning algorithms in tandem, which perform automated 3D measurement and analysis to extract key parameters of the weld seam, in conjunction with deep learning algorithms applied on image data of the weld seam for the automatic classification, positioning, and segmentation of the weld seam. The entire work is divided into the following: image acquisition, motion control, and image processing. Based on various weld seam detection algorithms, the selected model was MNet-based DeepLab-V3. An intelligent trimming system for welding seams based on deep learning was constructed. Experiments were conducted to verify the feasibility and accuracy of the 3D line laser measurement technology for weld seam inspections, and that the deep learning algorithm can effectively identify the type and location of the weld seam, thus predicting the trimming strategy. With an accuracy far superior to conventionally based methods in accurate detection and regrinding of weld surface defects, the system proves advantageous for improved weld regrinding productivity and quality. It was determined that the system presents significant advantages in reinforcing weld regrinding when it comes to efficiency and quality, thus initiating a paradigm of using intelligent treatments for medium/thick plate welds in the construction machinery industry.
Fusarium Species Shifts in Maize Grain as a Response to Climatic Changes in Poland
Authors: Czembor Elzbieta , Frasiński Seweryn , Urbaniak Monika , Waśkiewicz Agnieszka , Czembor Jerzy H. , Stępień Łukasz
Published: October 2024

Maize, along with wheat and rice, is the most important crop for food security. Ear rots caused by Fusarium species are among the most important diseases of maize. The distribution of Fusarium species provides essential epidemiological information for disease management. The effect of weather conditions, climate change and geographic localization on the Fusarium population in Poland was evaluated between 2015 and 2018. Grain samples (n = 233) were collected from hybrids at 16 locations (L1–L16). The differences in altitude between locations ranged from 39 to 243 m above sea level, longitude varied between 15°55′ and 23°12′ E, and latitude spanned from 50°12′ to 54°01′ N. Isolates were identified using molecular techniques. The highest Fusarium species frequency was recorded for 2016 (30.70%) and 2017 (28.18%), and the lowest for 2018 (5.36%). F. verticillioides and F. temperatum were the most frequent. Altitude has an effect on F. vericillioides frequency: F. graminearum showed a negative correlation with both latitude and longitude. In Polish conditions, from silking to harvesting, the number of days with higher precipitation and lower temperatures is associated with an increased frequency of F. verticillioides, F. temperatum, F. graminearum and F. avenaceum. This suggests that the Fusarium presence in Poland is significantly influenced not only by climate change but also by extreme weather changes.
Effects of Various Herbicide Types and Doses, Tillage Systems, and Nitrogen Rates on CO2 Emissions from Agricultural Land: A Literature Review
Authors: Hashim Zainulabdeen Khalaf , De Silva Agampodi Gihan Shyamal Dharmendra , Hassouni Ali Adnan , Vona Viktória Margit , Bede László , Stencinger Dávid , Horváth Bálint , Zsebő Sándor , Kulmány István Mihály
Published: October 2024

Although herbicides are essential for global agriculture and controlling weeds, they impact soil microbial communities and CO2 emissions. However, the effects of herbicides, tillage systems, and nitrogen fertilisation on CO2 emissions under different environmental conditions are poorly understood. This review explores how various agricultural practices and inputs affect CO2 emissions and addresses the impact of pest-management strategies, tillage systems, and nitrogen fertiliser usage on CO2 emissions using multiple databases. Key findings indicate that both increased and decreased tendencies in greenhouse gas (GHG) emissions were observed, depending on the herbicide type, dose, soil properties, and application methods. Several studies reported a positive correlation between CO2 emissions and increased agricultural production. Combining herbicides with other methods effectively controls emissions with minimal chemical inputs. Conservation practices like no-tillage were more effective than conventional tillage in mitigating carbon emissions. Integrated pest management, conservation tillage, and nitrogen fertiliser rate optimisation were shown to reduce herbicide use and soil greenhouse gas emissions. Fertilisers are similarly important; depending on the dosage, they may support yield or harm the soil. Fertiliser benefits are contingent on appropriate management practices for specific soil and field conditions. This review highlights the significance of adaptable management strategies that consider local environmental conditions and can guide future studies and inform policies to promote sustainable agriculture practices worldwide.
The Properties of Microorganisms and Plants in Soils after Amelioration
Authors: Zheng Xuebo
Published: October 2024

Soil, as a vital foundation of the Earth’s biosphere, supports plant growth, carbon storage, water cycling, and nutrient supply, making it a crucial resource for ensuring global food production and ecosystem health [...]
Response Mechanism and Evolution Trend of Carbon Effect in the Farmland Ecosystem of the Middle and Lower Reaches of the Yangtze River
Authors: Wang Xiaowen , Zheng Zhen , Jia Weidong , Tai Kaile , Xu Yujie , He Yuming
Published: October 2024

The farmland system in the global terrestrial ecosystem has dual attributes as both a carbon source and a carbon sink, playing a crucial role in controlling carbon emissions and mitigating global warming. Using carbon source and sink accounting of farmland ecosystems, we applied methods such as standard deviation ellipse, Tapio decoupling theory, and Markov chain to analyze the spatiotemporal changes, response mechanisms, and evolutionary trends of regional carbon effects. The results indicated that from 2011 to 2021, the farmland ecosystem in the middle and lower reaches of the Yangtze River consistently acted as a carbon sink. However, the net carbon sink showed slight fluctuations and significant spatial differences. The migration range of the net carbon sink center in the farmland ecosystem of the middle and lower reaches of the Yangtze River was relatively small, ranging from 115.52 to 115.77° E and 30.14 to 30.27° N. The decomposition of the Tapio decoupling index between the net carbon sink of the farmland ecosystem and agricultural output value showed the order of effects on their coupling relationship as follows: agricultural mechanization level > agricultural mechanization efficiency > agricultural output value > planting scale. The probability of maintaining the original state of net carbon sink in various cities in the middle and lower reaches of the Yangtze River (over 77%) was much higher than the probability of transfer, making it difficult to achieve a leapfrog growth in net carbon sink. The net carbon sink at the city scale exhibits the Matthew effect and spatial spillover effect. The above research results clarify the spatiotemporal changes in carbon effects in agricultural production at multiple levels, including city, province, and region. They also provide a theoretical basis for formulating differentiated regional emission reduction and sink enhancement strategies in the middle and lower reaches of the Yangtze River, promoting the rapid development of low-carbon agriculture in China.
Biochemical and Transcriptomic Analyses Reveal Key Salinity and Alkalinity Stress Response and Tolerance Pathways in Salix linearistipularis Inoculated with Trichoderma
Authors: Han Zhouqing , Chen Lili , Wang Wenyi , Guan Xueting , Song Junjie , Ma Shurong
Published: October 2024

Soil salinization and alkalinization are pervasive environmental issues that severely restrict plant growth and crop yield. Utilizing plant growth-promoting rhizobacteria (PGPR) is an effective strategy to enhance plant tolerance to saline–alkaline stress, though the regulatory mechanisms remain unclear. This study employed biochemical and RNA-Seq methods to uncover the critical growth-promoting effects of Trichoderma spp. on Salix linearistipularis under saline–alkaline stress. The results showed that, during saline–alkaline stress, inoculation with Trichoderma sp. M4 and M5 significantly increased the proline and soluble sugar contents in Salix linearistipularis, enhanced the activities of SOD, POD, CAT, and APX, and reduced lipid peroxidation levels, with M4 exhibiting more pronounced effects than M5. RNA-Seq analysis of revealed that 11,051 genes were upregulated after Trichoderma sp. M4 inoculation under stress conditions, with 3532 genes primarily involved in carbon metabolism, amino acid biosynthesis, and oxidative phosphorylation—processes that alleviate saline–alkaline stress. Additionally, 7519 genes were uniquely upregulated by M4 under stress, mainly enriched in secondary metabolite biosynthesis, amino acid metabolism, cyanamide metabolism, and phenylpropanoid biosynthesis. M4 mitigates saline–alkaline stress-induced damage in Salix linearistipularis seedlings by reducing oxidative damage, enhancing organic acid and amino acid metabolism, and activating phenylpropanoid biosynthesis pathways to eliminate harmful ROS. This enhances the seedlings’ tolerance to saline–alkaline stress, providing a basis for studying fungi–plant interactions under such conditions.
Influence of Contaminants Mercury and PAHs on Somatic Indexes of the European Hake (Merluccius merluccius, L. 1758)
Authors: Panfili Monica , Guicciardi o Guizzardi Stefano , Frapiccini Emanuela , Truzzi Cristina , Girolametti Federico , Marini Mauro , Santojanni Alberto , Annibaldi Anna , Illuminati Silvia , Colella Sabrina
Published: October 2024

This research investigates the dynamics of contaminant exposure in European hake (Merluccius merluccius, L. 1758) from the Adriatic Sea (Central Mediterranean Sea) by examining the levels of total mercury (THg) and polycyclic aromatic hydrocarbons (PAHs) in the muscle fish tissues. The study explores the correlations between these pollutants and somatic indexes to identify the early warning signals of pollution and ecological effects. The levels of pollutants are influenced by season and sex. Lipids appear to have a minimal effect on the PAH levels, whereas they exhibit a positive correlation with mercury levels in the muscle. No significant relationships between the pollutants and condition indexes were observed, except for a positive correlation between THg and the gonadosomatic index, indicating a potential impact on the reproductive health of fish. In contrast, PAHs showed no meaningful correlation with condition indexes. Differences in contaminant accumulations and lipid levels between sexes reflect variations in metabolic activity, reproductive costs, and adaptive strategies to seasonal changes and energy demands. This study highlights the importance of long-term monitoring to improve pollution management, environmental conservation, and the protection of marine organisms’ health.
Avian and Mammalian Diversity and Abundance in Jhalana Reserve Forest, Jaipur, India
Authors: Kumbhojkar Swapnil , Mahabal Anil , Rakholia Shrey , Yosef Reuven
Published: October 2024

We utilized camera traps to evaluate animal diversity, relative abundance, and the extent of anthropogenic activities in the Jhalana Reserve Forest (JRF), located in Jaipur, with a population of 3.9 million people. Between November 2017 and November 2019, camera traps were strategically deployed in the tourist zone and peripheral areas, capturing 16,328 photos. This study represents the first comprehensive baseline assessment of animal diversity in the JRF, documenting 39 species, including 18 bird species, 14 mammals, and 6 domestic species, alongside human activity. Among the 14 mammal species, 7 were carnivores. Notably, we identified 25 individual Indian leopards (Panthera pardus fusca) during 2017–2018, comprising 8 males and 17 females, highlighting the leopard as the apex predator in the JRF. Concurrently, domestic animals accompanied by humans were observed within the JRF. However, rigorous conservation efforts and patrols by the Rajasthan Forest Department resulted in a notable decline in human activity, from 28.04% in 2017–2018 to 3.92% in 2018–2019, with domestic animal activity reaching zero in the latter period. Consequently, the relative abundance of wildlife species increased during the study period of 2018–2019, underscoring the positive impact of conservation strategies implemented by authorities. Our findings establish that camera-trapping methodology collates definitive baseline data, assesses mammal diversity, and evaluates relative abundance in reserve forests within human-dominated landscapes. We strongly recommend a further study to assess the avifauna diversity. This study provides critical insights to inform the development and implementation of conservation strategies in similar protected areas.
Pyrroroquinoline Quinone (PQQ) Improves the Quality of Holstein Bull Semen during Cryopreservation
Authors: Wang Hai , Liu Kexiong , Zeng Weibin , Bai Jiahua , Xiao Linli , Qin Yusheng , Liu Yan , Xu Xiaoling
Published: October 2024

Cryopreserved semen is extensively utilized in the artificial insemination (AI) of domestic animals; however, suboptimal conception rates due to oxidative damage following AI continue to pose a challenge. The present study investigated the effects of Pyrroroquinoline Quinone (PQQ), a novel antioxidant, on the semen quality of Holstein bulls during cryopreservation, as well as its potential molecular mechanisms. Semen samples were diluted with varying concentrations of PQQ (0, 50 μmol/L, 100 μmol/L, 150 μmol/L) prior to cryopreservation. Following the freeze–thaw process, a comprehensive evaluation was conducted to assess sperm motility, plasma membrane integrity, acrosome integrity, and the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and adenosine triphosphate (ATP). Western blot analysis was employed to examine the levels of proteins including PGAM2, CAPZB, CAT, SOD1, and GPX1. Notably, the inclusion of 100 μmol/L PQQ significantly enhanced sperm motility, membrane integrity, and acrosome integrity post freeze–thawing (p < 0.05). Furthermore, the group treated with 100 μmol/L PQQ exhibited reduced levels of MDA and ROS (p < 0.05), while ATP levels were significantly elevated (p < 0.05). Interestingly, treatment with 100 μmol/L PQQ resulted in decreased consumption of PGAM2, CAPZB, CAT, SOD1, and GPX1 proteins in sperm after freeze–thawing, compared to the control group (p < 0.05). These findings indicate that PQQ treatment enhances the quality of bull semen, mitigates oxidative stress damage, and ultimately improves the efficacy of sperm cryopreservation.
Distribution and Seasonality of the Omura’s Whale (Balaenoptera omurai) in Australia Based on Passive Acoustic Recordings
Authors: Browne Ciara Edan , Erbe Christine , McCauley Robert D.
Published: October 2024

The Omura’s whale (Balaenoptera omurai) is one of the most recently described species of baleen whale. Initially known only from stranding and whaling specimens, it has now been identified in all ocean basins excluding the central and eastern Pacific. Unlike most baleen whales that migrate between the poles and the equator seasonally, the Omura’s whale is known to inhabit tropical to sub-tropical waters year-round. In Australian waters, there remain fewer than 30 confirmed visual sightings over the past decade. However, based on acoustic records, the Omura’s whale has been detected off areas of the northwest coast of Australia year-round. This study utilises passive acoustic recordings from 41 locations around Australia from 2005 to 2023 to assess the distribution and seasonality of the Omura’s whale. The seasonal presence of Omura’s whale vocalisations varied by location, with higher presence at lower latitudes. Vocalisations were detected year-round in the Joseph Bonaparte Gulf in the Timor Sea, and near Browse Island and Scott Reef, in the Kimberley region. In the Pilbara region, acoustic presence mostly peaked from February to April and no acoustic presence was consistently observed from July to September across all sites. The most southerly occurrence of Omura’s whale vocalisations was recorded off the North West Cape in the Gascoyne region. Vocalisations similar but not identical to those of the Omura’s whale were detected in the Great Barrier Reef. The identified seasonal distribution provides valuable information to assess environmental and anthropogenic pressures on the Omura’s whale and to aid in creating management and conservation policies for the species in Australia.
Transcriptome Analysis Reveals the Early Development in Subcutaneous Adipose Tissue of Laiwu Piglets
Authors: Bian Liwen , Di Zhaoyang , Xu Mengya , Tao Yuhan , Yu Fangyuan , Jiang Qingyan , Yin Yulong , Zhang Lin
Published: October 2024

Adipose tissue plays an important role in pig production efficiency. Studies have shown that postnatal development has a vital impact on adipose tissue; however, the mechanisms behind pig adipose tissue early-life programming remain unknown. In this study, we analyzed the transcriptomes of the subcutaneous adipose tissue (SAT) of 1-day and 21-day old Laiwu piglets. The results showed that the SAT of Laiwu piglets significantly increased from 1-day to 21-day, and transcriptome analysis showed that there were 2352 and 2596 differentially expressed genes (DEGs) between 1-day and 21-day SAT in male and female piglets, respectively. Expression of genes in glycolysis, gluconeogenesis, and glycogen metabolism such as pyruvate kinase M1/2 (PKM), phosphoenolpyruvate carboxy kinase 1 (PCK1) and amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) were significantly different between 1-day and 21-day SAT. Genes in lipid uptake, synthesis and lipolysis such as lipase E (LIPE), acetyl-CoA carboxylase alpha (ACACA), Stearoyl-CoA desaturase (SCD), and 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) were also differentially expressed. Functional analysis showed enrichment of DEGs in transcriptional regulation, protein metabolism and cellular signal transduction. The protein–protein interaction (PPI) networks of these DEGs were analyzed and potential hub genes in these pathways were identified, such as transcriptional factors forkhead box O4 (FOXO4), CCAAT enhancer binding protein beta (CEBPB) and CCAAT enhancer binding protein delta (CEBPD), signal kinases BUB1 mitotic checkpoint serine/threonine kinase (BUB1) and cyclin-dependent kinase 1 (CDK1), and proteostasis-related factors ubiquitin conjugating enzyme E2 C (UBE2C) and cathepsin D (CTSD). Moreover, we further analyzed the transcriptomes of SAT between genders and the results showed that there were 54 and 72 DEGs in 1-day and 21-day old SAT, respectively. Genes such as KDM5D and KDM6C showed gender-specific expression in 1-day and 21-day SAT. These results showed the significant changes in SAT between 1-day and 21-day in male and female Laiwu pigs, which would provide information to comprehensively understand the programming of adipose tissue early development and to regulate adipose tissue function.
Modeling Analysis of Nocturnal Nitrate Formation Pathways during Co-Occurrence of Ozone and PM2.5 Pollution in North China Plain
Authors: Dai Wei , Cheng Keqiang , Huang Xiangpeng , Xie Mingjie
Published: October 2024

The rapid formation of secondary nitrate (NO3−) contributes significantly to the nocturnal increase of PM2.5 and has been shown to be a critical factor for aerosol pollution in the North China Plain (NCP) region in summer. To explore the nocturnal NO3− formation pathways and the influence of ozone (O3) on NO3− production, the WRF-CMAQ model was utilized to simulate O3 and PM2.5 co-pollution events in the NCP region. The simulation results demonstrated that heterogeneous hydrolysis of dinitrogen pentoxide (N2O5) accounts for 60% to 67% of NO3− production at night (22:00 to 05:00) and is the main source of nocturnal NO3−. O3 enhances the formation of NO3 radicals, thereby further promoting nocturnal N2O5 production. In the evening (20:00 to 21:00), O3 sustains the formation of hydroxyl (OH) radicals, resulting in the reaction between OH radicals and nitrogen dioxide (NO2), which accounts for 48% to 64% of NO3− formation. Our results suggest that effective control of O3 pollution in NCP can also reduce NO3− formation at night.
Characterization of the Major Elements and Paleoenvironmental Significance of the Shiyang Profile in the Weinan Basin, China
Authors: Cao Jing , Dong Qiuyao , Li Chengxiang , Wang Pan , Yang Zhenjing , Chen Hongyun
Published: October 2024

The enrichment and migration patterns of different chemical elements record paleoclimatic information in loess formations. The chemical elemental measurements of 245 samples from the Shiyang profile in the Weinan Region were compared with the geochemical characteristics of typical wind-formed profiles, and the paleoclimatic evolution was discussed. The results showed the following: (1) the standardized curves of the cumulative concentrations of SiO2, Al2O3, and CaO along with the Upper Continental Crust (UCC) in the Shiyang profile exhibited significant similarities with typical wind-formed profiles. This strongly suggests that the Shiyang profile has a wind-formed origin. (2) The mean value of the chemical index of alteration (CIA) of the Shiyang profile is 62.06, indicating that the Shiyang profile has been in the stage of primary chemical weathering. (3) The ratios of K2O/Al2O3, TiO2/Al2O3, and Fe2O3/Al2O3 in the Shiyang profile are comparable to those found in typical wind-formed profiles, suggesting a common source area and supporting the premise that the Shiyang profile is of wind-induced origin.(4) The regional climate has undergone a series of transitions: from a dry and cool phase in the early Holocene to warm and humid yet unstable conditions in the middle Holocene, and returning to dry and cool during the late Holocene.