bluuebunny/crossref_metadata_2025 huggingface dataset:
Formyl-Peptide Receptor 2 Signaling Modulates SLC7A11/xCT Expression and Activity in Tumor Cells
Authors: Pecchillo Cimmino Tiziana , Punziano Carolina , Panico Iolanda , Petrone Zeudi , Cassese Myrhiam , Faraonio Raffaella , Barresi Vincenza , Esposito Gabriella , Ammendola Rosario , Cattaneo Fabio
Published: May 2024

Cancer cells exhibit high levels of oxidative stress and consequently require a high amount of cysteine for glutathione synthesis. Solute Carrier Family 7 Member 11 (SLC7A11), or xCT, mediates the cellular uptake of cystine in exchange for intracellular glutamate; imported extracellular cystine is reduced to cysteine in the cytosol through a NADPH-consuming reduction reaction. SLC7A11/xCT expression is under the control of stress-inducing conditions and of several transcription factors, such as NRF2 and ATF4. Formyl-peptide receptor 2 (FPR2) belongs to the FPR family, which transduces chemotactic signals mediating either inflammatory or anti-inflammatory responses according to the nature of its ligands and/or FPR2 binding with other FPR isoforms. The repertoire of FPR2 agonists with anti-inflammatory activities comprises WKYMVm peptide and Annexin A1 (ANXA1), and the downstream effects of the intracellular signaling cascades triggered by FPR2 include NADPH oxidase (NOX)-dependent generation of reactive oxygen species. Herein, we demonstrate that stimulation of CaLu-6 cells with either WKYMVm or ANXA1: (i) induces the redox-regulated activation of SLC7A11/xCT; (ii) promotes the synthesis of glutathione; (iii) prevents lipid peroxidation; and (iv) favors NRF2 nuclear translocation and activation. In conclusion, our overall results demonstrate that FPR2 agonists and NOX modulate SLC7A11/xCT expression and activity, thereby identifying a novel regulative pathway of the cystine/glutamate antiport that represents a new potential therapeutical target for the treatment of human cancers.
Variability in the Qualitative and Quantitative Composition of Phenolic Compounds and the In Vitro Antioxidant Activity of Sour Cherry (Prunus cerasus L.) Leaves
Authors: Zymonė Kristina , Liaudanskas Mindaugas , Lanauskas Juozas , Nagelytė Miglė , Janulis Valdimaras
Published: May 2024

Sour cherry (Prunus cerasus L.) is a deciduous tree belonging to the Rosaceae Juss. family. Cherry leaves are an underutilized source of biologically active compounds. The aim of this study was to determine the composition of the phenolic compounds, as well as the total antioxidant activity, in leaf samples of P. cerasus cultivars and to elucidate the cultivars with particular phytochemical compositions. The phytochemical profiles of P. cerasus leaves vary significantly in a cultivar-dependent manner. The total content of identified phenolic compounds varied from 8.254 to 16.199 mg/g in the cherry leaves. Chlorogenic acid ranged between 1413.3 µg/g (‘North Star’) and 8028.0 µg/g (‘Note’). The total content of flavonols varied from 4172.5 µg/g (‘Vytenu zvaigzde’) to 9030.7 µg/g (‘Tikhonovskaya’). The total content of identified proanthocyanidins varied from 122.3 µg/g (‘Note’) to 684.8 µg/g (‘Kelleris’). The highest levels of phloridzin (38.1 ± 0.9 µg/g) were found in samples of ‘Molodezhnaya’, while the lowest level of this compound was determined in the leaf samples of ‘Turgenevka’ (6.7 ± 0.2). The strongest antiradical (138.0 ± 4.0 µmol TE/g, p < 0.05) and reducing (364.9 ± 10.5 µmol TE/g, p < 0.05) activity in vitro was exhibited by the cultivar ‘Vytenu zvaigzde’ cherry leaf sample extracts. ‘Kelleris’, ‘Note’, and ‘Tikhonovskaya’ distinguish themselves with peculiar phytochemical compositions.
Astaxanthin Added during Post-Warm Recovery Mitigated Oxidative Stress in Bovine Vitrified Oocytes and Improved Quality of Resulting Blastocysts
Authors: Dujíčková Linda , Olexiková Lucia , Makarevich Alexander V. , Bartková Alexandra Rosenbaum , Němcová Lucie , Chrenek Peter , Strejček František
Published: May 2024

Various antioxidants are tested to improve the viability and development of cryopreserved oocytes, due to their known positive health effects. The aim of this study was to find whether astaxanthin (AX), a xanthophyll carotenoid, could mitigate deteriorations that occurred during the vitrification/warming process in bovine oocytes. Astaxanthin (2.5 µM) was added to the maturation medium during the post-warm recovery period of vitrified oocytes for 3 h. Afterward, the oocytes were fertilized in vitro using frozen bull semen and presumptive zygotes were cultured in the B2 Menezo medium in a co-culture with BRL-1 cells at 38.5 °C and 5% CO2 until the blastocyst stage. AX addition significantly reduced ROS formation, lipid peroxidation, and lysosomal activity, while increasing mitochondrial activity in vitrified oocytes. Although the effect of AX on embryo development was not observed, it stimulated cell proliferation in the blastocysts derived from vitrified oocytes and improved their quality by upregulation or downregulation of some genes related to apoptosis (BCL2, CAS9), oxidative stress (GPX4, CDX2), and development (GJB5) compared to the vitrified group without AX. Therefore, the antioxidant properties of astaxanthin even during short exposure to bovine vitrified/warmed oocytes resulted in improved blastocyst quality comparable to those from fresh oocytes.
Physical Activity and Oxidative Stress in Aging
Authors: Militello Rosamaria , Luti Simone , Gamberi Tania , Pellegrino Alessio , Modesti Alessandra , Modesti Pietro Amedeo
Published: May 2024

Biological aging, characterized by changes in metabolism and physicochemical properties of cells, has an impact on public health. Environment and lifestyle, including factors like diet and physical activity, seem to play a key role in healthy aging. Several studies have shown that regular physical activity can enhance antioxidant defense mechanisms, including the activity of enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase. However, intense or prolonged exercise can also lead to an increase in reactive oxygen species (ROS) production temporarily, resulting in oxidative stress. This phenomenon is referred to as “exercise-induced oxidative stress”. The relationship between physical activity and oxidative stress in aging is complex and depends on various factors such as the type, intensity, duration, and frequency of exercise, as well as individual differences in antioxidant capacity and adaptation to exercise. In this review, we analyzed what is reported by several authors regarding the role of physical activity on oxidative stress in the aging process as well as the role of hormesis and physical exercise as tools for the prevention and treatment of sarcopenia, an aging-related disease. Finally, we reported what has recently been studied in relation to the effect of physical activity and sport on aging in women.
Effectiveness of a Polyphenol-Enriched Blend on Weight Management and Metabolic Syndrome-Related Parameters in Healthy Overweight Adults
Authors: Martínez-Rodríguez Alejandro , Martínez-Olcina María , Vicente-Martínez Manuel , Asencio-Mas Nuria , Navarro Pau , Caturla Nuria , Jones Jonathan
Published: May 2024

Weight loss solutions are on the rise, and there is growing interest in natural alternatives to the pharmaceuticals in the market, which are not devoid of relevant side effects. To this end, the objective of the current study was to assess the effects of a botanical-based blend comprised of lemon verbena and hibiscus extracts on weight management, body fat, appetite, lipid, and glucose profiles in an overweight population for 90 days. The volunteers presented significant weight loss in absence of diet (approximately 3 kg, −3.4% total body weight), which coincided with decreased body fat (−1.7%, equivalent to an almost 6% decrease compared to initial value). Body fat was assessed by anthropometry, electrical bioimpedance and DEXA scans. Cholesterol levels were also significantly decreased (approximately 7% decrease). Satiety assessment revealed that the product contributed to increased feeling of fullness and satisfaction after a meal compared to the placebo group, which could contribute to the weight loss observed. Blood glucose and blood pressure levels remained unchanged, which was expected as the volunteers were normoglycemic and their blood pressure was in the healthy range. In conclusion, daily supplementation of a botanical-based blend contributes to weight loss in overweight individuals in absence of diet, while not affecting other parameters that are in the healthy range. This product could be a candidate alternative or add-on to other weight loss products as part of a healthy lifestyle.
The Advancement and Utilization of Marx Electric Field Generator for Protein Extraction and Inducing Structural Alterations
Authors: Stankevič Voitech , Jonynaitė Kamilė , Taha Ahmed , Keršulis Skirmantas , Dervinis Aldas , Kurčevskis Sebastjanas , Tolvaišienė Sonata , Stirkė Arūnas , Žurauskienė Nerija
Published: May 2024

This study introduces an innovative two-range, 12-stage Marx pulse generator employing thyristor switches designed specifically for the electroporation of biological cells. The generator consists of two module capacitors of different capacitances (1 μF and 0.25 μF), which enable the generation of electrical pulses with different durations and amplitudes of up to 25 kV. Safety aspects, including overcurrent and overvoltage protection mechanisms, are implemented in both the software and the hardware. In the experimental section, the tests of the Marx generator with resistive load are described in detail, and the results for the voltage fluctuations, pulse duration, and output characteristics of the generator are presented. The advantages of the design, including the high output voltage, the wide range of repetition rates, and the flexibility of the pulse parameters, are emphasized. Additionally, the research showcases the utilization of the devised generator for industrial purposes. Hence, an investigation into the efficiency of protein extraction from microalgae (Chlorella vulgaris) and the impacts of pulsed electric fields (PEFs) on the structural characteristics of casein micelles (CSMs) was chosen as an illustrative example. The obtained results provide valuable insights into the application of PEF in food processing and biotechnology and underline the potential of the developed generator for sustainable and environmentally friendly practices.
Inadvertent Tooth Movement from a Bonded Mandibular Lingual Retainer—A Case Report with a Follow-Up 3D Analysis of Tooth Movement and a Microscopic Evaluation of the Wire
Authors: Jedliński Maciej , Tandecka Katarzyna , Grocholewicz Katarzyna , Janiszewska-Olszowska Joanna
Published: May 2024

Background: One of the rarest complications of fixed orthodontic retention is inadvertent tooth movement of the teeth bonded to the retainer. A 25-year-old patient presented at the orthodontist as she was preoccupied about the position of the lower teeth. The aim of this case report was to present a follow-up of anterior teeth alignment after fixed retainer removal and to analyze the structure of the removed fixed orthodontic retainer in a patient suffering from a twist effect. Materials and Methods: The retainer that caused inadvertent movement has been removed, and subsequent teeth displacement was assessed with scan superimposition. The retainer structure and diameter were analyzed with a laser confocal microscope. Results: The superimposition showed significant improvements in the position of the teeth. The sole removal of the activated retainer resulted in a partial self-correction of the twist effect. Under microscopic observation, changes in the dimensions of the wire were found but were too small to cause significant changes in tooth position. Conclusions: A fixed retainer should always be removed when a twist effect is diagnosed. The wire sections covered with composite are less likely to untwist. In the presented case, the changes in the dimensions of the round retainer wire could not have led to changes in the teeth’s positions.
Adaptive Shadow Compensation Method in Hyperspectral Images via Multi-Exposure Fusion and Edge Fusion
Authors: Meng Yan , Li  Guanyi , Huang Wei
Published: May 2024

Shadows in hyperspectral images lead to reduced spectral intensity and changes in spectral characteristics, significantly hindering analysis and applications. However, current shadow compensation methods face the issue of nonlinear attenuation at different wavelengths and unnatural transitions at the shadow boundary. To address these challenges, we propose a two-stage shadow compensation method based on multi-exposure fusion and edge fusion. Initially, shadow regions are identified through color space conversion and an adaptive threshold. The first stage utilizes multi-exposure, generating a series of exposure images through adaptive exposure coefficients that reflect spatial shadow intensity variations. Fusion weights for exposure images are determined based on exposure, contrast, and spectral variance. Then, the exposure sequence and fusion weights are constructed as Laplacian pyramids and Gaussian pyramids, respectively, to obtain a weighted fused exposure sequence. In the second stage, the previously identified shadow regions are smoothly reintegrated into the original image using edge fusion based on the p-Laplacian operator. To further validate the effectiveness and spectral fidelity of our method, we introduce a new hyperspectral image dataset. Experimental results on the public dataset and proposed dataset demonstrate that our method surpasses other mainstream shadow compensation methods.
Reassessing the Location, Magnitude, and Macroseismic Intensity Map of the 8 April 1893 Svilajnac (Serbia) Earthquake
Authors: Manić Miodrag I. , Bulajić Borko Đ.
Published: May 2024

A devastating earthquake took place on 8 April 1893, close to the town of Svilajnac, central Serbia. Over the past decade, significant historical data on the effects of this earthquake has been collected from a variety of sources, including books, scientific publications, reports, newspapers, and coeval chronicles. Additionally, this earthquake was recorded 750 km from the epicenter at the seismological station Rocca di Papa in Rome, Italy. Based on critical review and analysis of the historical data, we demonstrate that the epicentral area of this earthquake was 531 km2, and the macroseismic effects were recorded at epicentral distances up to 600 km towards the west (Vienna, Austria) towards the north, up to 500 km (Košice–Michalovce, Slovakia), towards the east up to 460 km (Brašov–Borsec, Romania); and towards the south up to about 300 km (Radoviš, North Macedonia). Finally, we show that the key parameters of the 1893 Svilajnac earthquake are as follows: (1) epicentral intensity, I0 = IX EMS-98, (2) the estimations of the moment magnitude and focal depth based on the observed intensities, MW = 6.8 and h = 13 km, respectively, and (3) the epicenter coordinates, 44.160° N and 21.354° E.
Spatiotemporal Dynamics of CO2 Emissions in China Based on Multivariate Spatial Statistics
Authors: Wang Mengyao , Dai Xiaoyan , Zhang Hao
Published: May 2024

With China’s rapid industrialization and urbanization in the process of socio-economic development, the extensive use of energy has resulted in a large amount of CO2 emissions, which puts great pressure on China’s carbon emission reduction task. Through multivariate socio-economic data, this paper proposes an extraction and screening method of multivariate variables based on land-use types, and the downscaled spatial decomposition of carbon emissions at different scales was carried out by using the spatial lag model (SLM). This paper makes up for the shortcomings of previous studies, such as an insufficient modeling scale, simple modeling variables, limited spatio-temporal span of spatial decomposition, and no consideration of geographical correlation. Based on the results of the spatial decomposition of carbon emissions, this paper explores the spatial and temporal dynamics of carbon emissions at different scales. The results showed that SLM is capable of downscaling the spatialization of carbon emissions with high precision, and the continuity of the decomposition results at the provincial scale is stronger, while the differences of the decomposition results at the municipal scale are more obvious within the municipal units. In terms of the spatial and temporal dynamics of CO2 emissions, carbon emissions at both scales showed a significant positive correlation. The dominant spatial correlation types are “Low–Low” at the provincial level, and “Low–Low” and “High–High” at the municipal level. The smaller spatial scope is more helpful to show the geographic dependence and geographic differences of China’s carbon emissions. The findings of this paper will help deepen the understanding of the spatial and temporal changes of carbon emissions in China. They will provide a scientific basis for the formulation of feasible carbon emission reduction policies.
The Relationship between Changes in Hydro-Climate Factors and Maize Crop Production in the Equatorial African Region from 1980 to 2021
Authors: Nooni Isaac Kwesi , Ogou Faustin Katchele , Hagan Daniel Fiifi Tawiah , Saidou Chaibou Abdoul Aziz , Prempeh Nana Agyemang , Nakoty Francis Mawuli , Jin Zhongfang , Lu Jiao
Published: May 2024

Agricultural production across the African continent is subjected to various effects of climate variability. One of the main staple foods in Sub-Saharan Africa is maize. However, limited scientific research has recently focused on understanding the possible effects of hydro-climatic variability on maize production. The aim of the present work was to contribute to policy and climate adaptation, thus reducing the vulnerability of maize production to climate change over Equatorial Africa. This study firstly examined long-term trends of precipitation (PRE), soil moisture (SM), actual evapotranspiration (E), and potential evapotranspiration (Ep), as well as surface air temperatures, including the minimum (TMIN) and maximum (TMAX). Secondly, the relationship between maize production and these climate variables was quantified for 18 Equatorial African countries (EQCs) over 1980−2021. To assess the linear trends, Mann–Kendall and Sen’s slope tests were used to quantify the magnitude of the hydro-climatic variable trends at the 5% significance level, and Pearson’s correlation coefficient was used to evaluate the relation of these climate parameters with the maize production. The annual mean PRE declined at 0.03 mm day−110a−1. Other climate variables increased at different rates: SM at 0.02 mmday−110a−1, E at 0.03 mm day−110a−1, Ep at 0.02 mm day−1 10a−1, TMIN and TMAX at 0.01 °C day−110a−1. A regional analysis revealed heterogeneous significant wet–dry and warm–cool trends over the EQCs. While, spatially, dry and warm climates were observed in the central to eastern areas, wet and warm conditions dominated the western regions. Generally, the correlations of maize production with the E, Ep, TMAX, and TMIN were strong (r > 0.7) and positive, while moderate (r > 0.45) correlations of maize production with PRE and SM were obvious. These country-wide analyses highlight the significance of climate change policies and offer a scientific basis for designing tailored adaptation strategies in rainfed agricultural regions.
Characteristics of the East Asian Summer Monsoon Using GK2A Satellite Data
Authors: Wie Jieun , Byon Jae-Young , Moon Byung-Kwon
Published: May 2024

In East Asia, where concentrated summer precipitation often leads to climate disasters, understanding the factors that cause such extreme rainfall is crucial for effective forecasting and preparedness. The western North Pacific subtropical high (WNPSH) is a key driver of summer precipitation variability, and therefore, its monitoring is critical to predicting the wet or dry periods during the East Asian summer monsoon. Using the Geo-KOMPSAT 2A (GK2A) satellite cloud amount data and ERA5 reanalysis data during the years 2020–2023, this study identified three leading empirical orthogonal function (EOF) modes and investigated the associated WNPSH variability at synoptic and subseasonal scales. The analysis includes a linear regression of meteorological fields onto the principal component (PC) time series. All three modes play a role in the spatiotemporal variability of the WNPSH, exhibiting lead–lag relationships. In particular, the second mode is responsible for its northwestward shift and intensification. As the WNPSH moves northwestward, the position of the monsoon rain band also shifts, and its intensity is modulated mainly by the moisture transport along the WNPSH boundary. Our results highlight the potential of high-resolution, real-time data from the GK2A satellite to elucidate WNPSH variability and its impact on the East Asian summer monsoon. By addressing the variability of the WNSPH using GK2A data, we pave the way for the development of a real-time monitoring framework with GK2A, which will improve our predictability and readiness for extreme weather events in East Asia.
Investigation of BTX Concentrations and Effects of Meteorological Parameters in the Steelpoort Area of Limpopo Province, South Africa
Authors: Maswanganyi Collet , Tshilongo James , Mkhohlakali Andile , Martin Lynwill
Published: May 2024

It has been demonstrated that benzene, toluene, and xylene are carcinogens. Its combined effects with other contaminants have the potential to harm several ecosystem components. Since most human benzene exposure takes place inside, it is important to understand how outdoor benzene emissions from traffic and industry affect interior concentrations. However, this area of study has not received enough attention to date. Herein, we examine the outdoor concentrations of benzene, toluene, and xylene (BTX) in a Steelpoort mining area. BTX pollutants were passively sampled on the first seven days of the month, from January to December 2021 using Radiello samplers. The effects of meteorological parameters such as temperature, relative humidity, wind speed, and solar radiation on BTX concentrations were also statistically tested. For all seasons, BTX concentrations were greater in the winter than in the summer with concentrations of 0.69 µg/m3, 2.97 µg/m3 and 0.80 µg/m3 for benzene, toluene and xylene, respectively. In addition, toluene was the most common BTX compound with the highest concentrations when compared to benzene and xylene. Benzene, toluene and xylene, had yearly average concentrations of 0.61 µg/m3, 1.48 µg/m3 and 0.64 µg/m3, respectively. The benzene and xylene concentrations were below international exposure limits (annual, 5 µg/m3 for benzene; weekly, 260 µg/m3 for toluene), as in comparison to the World Health Organization, as well as within South African exceedance limits. Both positive and negative correlations between BTX and meteorological parameters were demonstrated by statistical models. Temperature, wind speed, and relative humidity depicted a weak negative correlation with benzene of 0.003, 0.019 and 0.006, respectively. Toluene showed a positive correlation with wind speed (1.90) and relative humidity (0.041). Overall, the concentration of benzene is of major concern since it is an agent of cancer and it is there in the atmosphere.
Distinguishing Saharan Dust Plume Sources in the Tropical Atlantic Using Elemental Indicators
Authors: Yeager Daniel E. , Morris Vernon R.
Published: May 2024

The Sahara Desert is the largest contributor of global atmospheric dust aerosols impacting regional climate, health, and ecosystems. The climate effects of these dust aerosols remain uncertain due, in part, to climate model uncertainty of Saharan source region contributions and aerosol microphysical properties. This study distinguishes source region elemental signatures of Saharan dust aerosols sampled during the 2015 Aerosols Ocean Sciences Expedition (AEROSE) in the tropical Atlantic. During the 4-week campaign, cascade impactors size-dependently collected airborne Saharan dust particulate upon glass microfiber filters. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis differentiated metal isotope concentrations within filter samples from various AEROSE dust sampling periods. Back-trajectory analysis and NOAA satellite aerosol optical depth retrievals confirmed source regions of AEROSE ’15 dust samples. Pearson correlational statistics of source region activity and dust isotope concentrations distinguished the elemental signatures of North African potential source areas (PSAs). This study confirmed that elemental indicators of these PSAs remain detectable within dust samples collected far into the marine boundary layer of the tropical Atlantic. Changes detected in dust elemental indicators occurred on sub-weekly timescales across relatively small sampling distances along the 23W parallel of the tropical Atlantic. PSA-2 emissions, covering the western coast of the Sahara, were very strongly correlated (R2 > 0.79) with Ca-44 isotope ratios in AEROSE dust samples; PSA-2.5 emissions, covering eastern Mauritania and western Mali, were very strongly correlated with K-39 ratios; PSA-3 emissions, spanning southwestern Algeria and eastern Mali, were very strongly correlated with Fe-57 and Ti-48 ratios. The abundance of Ca isotopes from PSA-2 was attributed to calcite minerals from dry lakebeds and phosphorous mining activities in Western Sahara, based on source region analysis. The correlation between K isotope ratios and PSA-2.5 was a likely indicator of illite minerals near the El Djouf Desert region, according to corroboration with mineral mapping studies. Fe and Ti ratio correlations with PSA-3 observed in this study were likely indicators of iron and titanium oxides from Sahelian sources still detectable in Atlantic Ocean observations. The rapid changes in isotope chemistry found in AEROSE dust samples provide a unique marker of Saharan source regions and their relative contributions to desert outflows in the Atlantic. These elemental indicators provide source region apportionments of Sahara Desert aerosol flux and deposition into the Atlantic Ocean, as well as a basis for model and satellite validation of Saharan dust emissions for regional climate assessments.
Evaluating Phoenix Metropolitan Area Ozone Behavior Using Ground-Based Sampling, Modeling, and Satellite Retrievals
Authors: Miech Jason , Herckes Pierre , Fraser Matthew , Arellano Avelino , Mirrezaei Mohammad , Guo Yafang
Published: May 2024

An oxidizing and harmful pollutant gas, tropospheric ozone is a product of a complex set of photochemical reactions that can make it difficult to enact effective control measures. A better understanding of its precursors including volatile organic compounds (VOCs) and nitrogen oxides (NOx) and their spatial distribution can enable policymakers to focus their control efforts. In this study we used low-cost sensors (LCSs) to increase the spatial resolution of an existing NO2 monitoring network in addition to VOC sampling to better understand summer ozone formation in Maricopa County, Arizona, and observed that afternoon O3 values at the downwind sites were significantly correlated, ~0.27, to the morning NO2 × rate values at the urban sites. Additionally, we looked at the impact of wildfire smoke on ozone exceedances and compared non-smoke days to smoke days. The average O3 on smoke days was approximately 20% higher than on non-smoke days, however, the average NO2 concentration multiplied by estimated photolysis rate (NO2 × rate) values were only 2% higher on smoke days. Finally, we evaluated the ozone sensitivity of the region by calculating HCHO/NO2 ratios using three different datasets: ground, satellite, and model. Although the satellite dataset produced higher HCHO/NO2 ratios than the other datasets, when the proper regime thresholds are applied the three datasets consistently show transition and VOC-limited O3 production regimes over the Phoenix metro area. This suggests a need to implement more VOC emission controls in order to reach O3 attainment in the county.
A Year-Long Measurement and Source Contributions of Volatile Organic Compounds in Nanning, South China
Authors: Wu Ying , Mo Zhaoyu , Wu Qinqin , Fan Yongji , Chen Xuemei , Li Hongjiao , Lin Hua , Huang Xishou , Tang Hualei , Liao Donglan , Liu Huilin , Mo Ziwei
Published: May 2024

Severe ozone (O3) pollution has been recorded in China in recent years. The key precursor, volatile organic compounds (VOCs), is still not well understood in Nanning, which is a less developed city compared to other megacities in China. In this study, a year-long measurement of VOCs was conducted from 1 October 2020 to 30 September 2021, to characterize the ambient variations and apportion the source contributions of VOCs. The daily-averaged concentration of VOCs was measured to be 26.4 ppb, ranging from 3.2 ppb to 136.2 ppb across the whole year. Alkanes and oxygenated VOCs (OVOCs) were major species, contributing 46.9% and 25.2% of total VOC concentrations, respectively. Propane, ethane, and ethanol were the most abundant in Nanning, which differed from the other significant species, such as toluene (3.7 ppb) in Guangzhou, ethylene (3.8 ppb) in Nanjing, and isopentane (5.5 ppb), in Chengdu. The positive matrix factorization (PMF) model resolved six source factors, including vehicular emission (contributing 33% of total VOCs), NG and LPG combustion (19%), fuel burning (17%), solvent use (16%), industry emission (10%), and biogenic emission (5%). This indicated that Nanning was less affected by industrial emission compared with other megacities of China, with industry contributing 12–50%. Ethylene, m/p-xylene, butane, propylene, and isoprene were key species determined by ozone formation potential (OFP) analysis, which should be priority-controlled. The variations in estimated OFP and observed O3 concentrations were significantly different, suggesting that VOC reactivity-based strategies as well as meteorological and NOx effects should be considered collectively in controlling O3 pollution. This study presents a year-long dataset of VOC measurements in Nanning, which gives valuable implications for VOC control in terms of key sources and reactive species and is also beneficial to the formulation of effective ozone control strategies in other less developed regions of China.
Climate Change, Air Pollution, and Human Health in the Kruger to Canyons Biosphere Region, South Africa, and Amazonas, Brazil: A Narrative Review
Authors: dos Santos Monika
Published: May 2024

There is a 50% possibility that global temperatures will have risen by more than 5 °C by the year 2100. As demands on Earth’s systems grow more unsustainable, human security is clearly at stake. This narrative review provides an overview and synthesis of findings in relation to climate change, air pollution, and human health within the Global South context, focusing on case study geographic locations in South Africa and Brazil. Two case study regions—the Kruger to Canyons Biosphere region of South Africa and the Amazon region of Brazil—were the subjects of PubMed literature searches. Technical reports, policy briefs, and grey literature were also narratively synthesized. The burning of wood for fuel, as witnessed in Agincourt, and forest fires, such as those seen in the Amazon rainforest, release air pollutants such as methane and black carbon, which are strong short-lived climate pollutants (SLCPs) which fuel climate change and adversely affect human health. SLCPs have a brief lifetime in the atmosphere, but they frequently have a far larger potential for global warming than carbon dioxide (CO2). Most air pollution in geographic case study areas, that are home to human settlements, is due to the burning of wood and other biomasses that are pollutants. These areas are seen to be important for climate and health responses, and if constructive action is taken to switch to other modes of electricity generation (such as solar power) and the prevention of deforestation, the worst of the impacts may still be mitigated in these regions. Authorities should also establish a monitoring strategy for air quality, as well as enforce air quality regulations that safeguard public health.
Wide and Deep Learning Model for Satellite-Based Real-Time Aerosol Retrievals in China
Authors: Luo Nana , Zou Junxiao , Zang Zhou , Chen Tianyi , Yan Xing
Published: May 2024

Machine learning methods have been recognized as rapid methods for satellite-based aerosol retrievals but have not been widely applied in geostationary satellites. In this study, we developed a wide and deep learning model to retrieve the aerosol optical depth (AOD) using Himawari-8. Compared to traditional deep learning methods, we embedded a “wide” modeling component and tested the proposed model across China using independent training (2016–2018) and test (2019) datasets. The results showed that the “wide” model improves the accuracy and enhances model interpretability. The estimates exhibited better accuracy (R2 = 0.81, root-mean-square errors (RMSEs) = 0.19, and within the estimated error (EE) = 63%) than those of the deep-only models (R2 = 0.78, RMSE = 0.21, within the EE = 58%). In comparison with extreme gradient boosting (XGBoost) and Himawari-8 V2.1 AOD products, there were also significant improvements. In addition to higher accuracy, the interpretability of the proposed model was superior to that of the deep-only model. Compared with other seasons, higher contributions of spring to the AOD concentrations were interpreted. Based on the application of the wide and deep learning model, the near-real-time variation of the AOD over China could be captured with an ultrafine temporal resolution.
A New Nonlinear Integral Inequality with a Tempered Ψ–Hilfer Fractional Integral and Its Application to a Class of Tempered Ψ–Caputo Fractional Differential Equations
Authors: Medved’ Milan , Pospíšil Michal , Brestovanská Eva
Published: May 2024

In this paper, the tempered Ψ–Riemann–Liouville fractional derivative and the tempered Ψ–Caputo fractional derivative of order n−1<α<n∈N are introduced for Cn−1–functions. A nonlinear version of the second Henry–Gronwall inequality for integral inequalities with the tempered Ψ–Hilfer fractional integral is derived. By using this inequality, an existence and uniqueness result and a sufficient condition for the non-existence of blow-up solutions of nonlinear tempered Ψ–Caputo fractional differential equations are proved. Illustrative examples are given.
MSC-Based Cell Therapy in Neurological Diseases: A Concise Review of the Literature in Pre-Clinical and Clinical Research
Authors: Zhang Xiaorui , Kuang Qihong , Xu Jianguang , Lin Qing , Chi Haoming , Yu Daojin
Published: May 2024

Mesenchymal stem cells (MSCs) are multipotent stromal cells with the ability to self-renew and multi-directional differentiation potential. Exogenously administered MSCs can migrate to damaged tissue sites and participate in the repair of damaged tissues. A large number of pre-clinical studies and clinical trials have demonstrated that MSCs have the potential to treat the abnormalities of congenital nervous system and neurodegenerative diseases. Therefore, MSCs hold great promise in the treatment of neurological diseases. Here, we summarize and highlight current progress in the understanding of the underlying mechanisms and strategies of MSC application in neurological diseases.
A Tiny Viral Protein, SARS-CoV-2-ORF7b: Functional Molecular Mechanisms
Authors: Mansueto Gelsomina , Fusco Giovanna , Colonna Giovanni
Published: May 2024

This study presents the interaction with the human host metabolism of SARS-CoV-2 ORF7b protein (43 aa), using a protein–protein interaction network analysis. After pruning, we selected from BioGRID the 51 most significant proteins among 2753 proven interactions and 1708 interactors specific to ORF7b. We used these proteins as functional seeds, and we obtained a significant network of 551 nodes via STRING. We performed topological analysis and calculated topological distributions by Cytoscape. By following a hub-and-spoke network architectural model, we were able to identify seven proteins that ranked high as hubs and an additional seven as bottlenecks. Through this interaction model, we identified significant GO-processes (5057 terms in 15 categories) induced in human metabolism by ORF7b. We discovered high statistical significance processes of dysregulated molecular cell mechanisms caused by acting ORF7b. We detected disease-related human proteins and their involvement in metabolic roles, how they relate in a distorted way to signaling and/or functional systems, in particular intra- and inter-cellular signaling systems, and the molecular mechanisms that supervise programmed cell death, with mechanisms similar to that of cancer metastasis diffusion. A cluster analysis showed 10 compact and significant functional clusters, where two of them overlap in a Giant Connected Component core of 206 total nodes. These two clusters contain most of the high-rank nodes. ORF7b acts through these two clusters, inducing most of the metabolic dysregulation. We conducted a co-regulation and transcriptional analysis by hub and bottleneck proteins. This analysis allowed us to define the transcription factors and miRNAs that control the high-ranking proteins and the dysregulated processes within the limits of the poor knowledge that these sectors still impose.
Scorpion Venom Antimicrobial Peptide Derivative BmKn2-T5 Inhibits Enterovirus 71 in the Early Stages of the Viral Life Cycle In Vitro
Authors: Xia Zhiqiang , Wang Huijuan , Chen Weilie , Wang Aili , Cao Zhijian
Published: May 2024

Enterovirus 71 (EV71), a typical representative of unenveloped RNA viruses, is the main pathogenic factor responsible for hand, foot, and mouth disease (HFMD) in infants. This disease seriously threatens the health and lives of humans worldwide, especially in the Asia–Pacific region. Numerous animal antimicrobial peptides have been found with protective functions against viruses, bacteria, fungi, parasites, and other pathogens, but there are few studies on the use of scorpion-derived antimicrobial peptides against unenveloped viruses. Here, we investigated the antiviral activities of scorpion venom antimicrobial peptide BmKn2 and five derivatives, finding that BmKn2 and its derivative BmKn2-T5 exhibit a significant inhibitory effect on EV71. Although both peptides exhibit characteristics typical of amphiphilic α-helices in terms of their secondary structure, BmKn2-T5 displayed lower cellular cytotoxicity than BmKn2. BmKn2-T5 was further found to inhibit EV71 in a dose-dependent manner in vitro. Moreover, time-of-drug-addition experiments showed that BmKn2-T5 mainly restricts EV71, but not its virion or replication, at the early stages of the viral cycle. Interestingly, BmKn2-T5 was also found to suppress the replication of the enveloped viruses DENV, ZIKV, and HSV-1 in the early stages of the viral cycle, which suggests they may share a common early infection step with EV71. Together, the results of our study identified that the scorpion-derived antimicrobial peptide BmKn2-T5 showed valuable antiviral properties against EV71 in vitro, but also against other enveloped viruses, making it a potential new candidate therapeutic molecule.
Pipeline Terracotta Microbial Fuel Cell: Organic Content Biosensor and Energy Harvesting Device Integrated in Wastewater Pipeline
Authors: Nakamoto Trang , Nakamoto Dung , Taguchi Kozo
Published: May 2024

Wastewater pipelines are present everywhere in urban areas. Wastewater is a preferable fuel for renewable electricity generation from microbial fuel cells. Here, we created an integrated microbial fuel cell pipeline (MFCP) that could be connected to wastewater pipelines and work as an organic content biosensor and energy harvesting device at domestic waste-treatment plants. The MFCP used a pipeline-like terracotta-based membrane, which provided structural support for the MFCP. In addition, the anode and cathode were attached to the inside and outside of the terracotta membrane, respectively. Co−MnO2 was used as a catalyst to improve the performance of the MFCP cathode. The experimental data showed a good linear relationship between wastewater chemical oxygen demand (COD) concentration and the MFCP output voltage in a COD range of 200–1900 mg/L. This result implies the potential of using the MFCP as a sensor to detect the organic content of the wastewater inside the wastewater pipeline. Furthermore, the MFCP can be used as a long-lasting sustainable energy harvester with a maximum power density of 400 mW/m2 harvested from 1900 mg/L COD wastewater at 25 °C.
A Comprehensive Review of Organ-on-a-Chip Technology and Its Applications
Authors: Farhang Doost Negar , Srivastava Soumya K.
Published: May 2024

Organ-on-a-chip (OOC) is an emerging technology that simulates an artificial organ within a microfluidic cell culture chip. Current cell biology research focuses on in vitro cell cultures due to various limitations of in vivo testing. Unfortunately, in-vitro cell culturing fails to provide an accurate microenvironment, and in vivo cell culturing is expensive and has historically been a source of ethical controversy. OOC aims to overcome these shortcomings and provide the best of both in vivo and in vitro cell culture research. The critical component of the OOC design is utilizing microfluidics to ensure a stable concentration gradient, dynamic mechanical stress modeling, and accurate reconstruction of a cellular microenvironment. OOC also has the advantage of complete observation and control of the system, which is impossible to recreate in in-vivo research. Multiple throughputs, channels, membranes, and chambers are constructed in a polydimethylsiloxane (PDMS) array to simulate various organs on a chip. Various experiments can be performed utilizing OOC technology, including drug delivery research and toxicology. Current technological expansions involve multiple organ microenvironments on a single chip, allowing for studying inter-tissue interactions. Other developments in the OOC technology include finding a more suitable material as a replacement for PDMS and minimizing artefactual error and non-translatable differences.
Evaluating Two Brief Motivational Interventions for Excessive-Drinking University Students
Authors: Hogan Lee M. , Cox W. Miles
Published: May 2024

Objective: Two brief computerized motivational interventions for excessive-drinking university students were evaluated. Method: Participants (N = 88, females = 61.5%, mean age = 21.05 years) were randomly assigned to a control group or one of two experimental groups: Computerized Brief Intervention (CBI) or Computerized Brief Intervention-Enhanced (CBI-E). CBI followed the principles of Motivational Interviewing to motivate participants to change their drinking behavior. CBI-E additionally used the principles of Systematic Motivational Counseling to identify and discuss with participants their dysfunctional motivational patterns that were interfering with their attainment of emotional satisfaction. At baseline and a three-month follow-up, the participants completed a battery of measures of alcohol consumption and related problems. Results: At baseline, the participants were confirmed to be heavy drinkers with many drink-related negative consequences. Males and females responded differently to the interventions. During follow-up, males’ alcohol use was ordered: CBI-E < CBI < Controls. The females in all three groups reduced their alcohol use, but there were no significant group differences. Conclusions: Males responded to the interventions as expected. For females, the assessment itself seemed to serve as an effective intervention, and there were no post-intervention differences among the three groups. Suggestions for future research using CBI and CBI-E are discussed.
The Influence of Personality Type D and Coping Strategies on Cognitive Functioning in Students
Authors: Sumin Alexey N. , Prokashko Ingrid Yu. , Shcheglova Anna V.
Published: May 2024

Introduction: Academic and emotional challenges faced by medical students can affect their psychological well-being and health. Personal characteristics may also predispose one to the manifestation of distress reactions. Individuals with type D personality have an increased tendency to develop depressive reactions and somatic diseases, including the presence of cognitive dysfunction. In students, the presence of cognitive dysfunction may additionally adversely affect academic and psycho-emotional problems. The purpose of this study was to examine the influence of type D personality and coping strategies on cognitive functioning in medical students. Methods: A cross-sectional study included 258 medical students (age 19 ± 1.2 years, 79 men). All participants completed psychological questionnaires (DS-14 to identify type D personality, and The Coping Strategy Indication, CSI—to determine coping strategies), as well as extensive neuropsychological testing of cognitive functions. Results: Among the medical students examined, the frequency of identification of type D personality was 44%. In persons with personality type D, according to psychometric testing, a decrease in the level of functional mobility of nervous processes (FMNP) was noted, which was manifested in an increase in the test completion time (p < 0.001) and an increase in the number of errors (p < 0.001) during the FMNP test, and an increase in the test completion time in the attention concentration test. In addition, in type D participants, an increase in the test execution time during the attention test was noted (p = 0.007). Personality type D was an independent risk factor for cognitive decline in students in multiple linear regression analysis, when type D was analyzed as a dichotomous construct. Conclusions: Assessing personal characteristics and identifying personality type D is advisable for medical students, to develop subsequent programs to increase their resistance to academic challenges, improve cognitive function, and also to prepare for future stress loads during professional activities in the field of healthcare.