bluuebunny/crossref_metadata_2025 huggingface dataset:
3. Kinship Denied and Acknowledged
Authors: Sommer Marianne
Published: July 2024

While there was a way of using diagrams to evidence close human kinship, diagrams were thus also used to deny genealogical relatedness, to create differences within humankind that amounted to the status of unrelated species. Samuel George Morton drew especially on French polygenist writings, in which a rather elaborate system of measurements was already in place. This diagrammatics of ‘race’ was enmeshed in matters of ‘racial’ politics. At the same time, Morton also referred to the works of monogenists, and members of both camps could stand for complex and contradicting politics of knowledge.
8. Map, Scale, and Tree in Darwin, Haeckel and Co.
Authors: Sommer Marianne
Published: July 2024

The coexistence of different diagrammatic elements also characterized Darwin’s verbal and visual imagery. Charles Darwin was inspired by family genealogy in his understanding of humanity at large and of the entire living world. However, even though Darwin drew a family tree of the primates, he did not include it in The Descent of Man (1871). Those who preceded Darwin in the application of antiquity and evolution to humankind, too, did not include human phylogenies in their pioneering books. The exception was ‘the German Darwin’, i.e. Ernst Haeckel, whose phylogenies Darwin considered too speculative.
Part III. Radicalizing versus Deconstructing the Family Tree of the Human ‘Races’
Authors: Sommer Marianne
Published: July 2024

As shown in Part II, there in fact existed theories of human classification and evolution in the history of anthropology that corresponded to the notions transported by the human family tree, such as the independent evolution of human populations. Sometimes explicitly drawing on Ernst Haeckel’s phylogenies, some anthropologists published polygenist diagrams to convey their understanding of intra-human specific or even generic differences well into the twentieth century. In these visualization practices, the tree shape was increasingly radicalized through prolongation of the independent lines leading to the modern groups that were thereby constructed in hierarchical order. At the same time, while there have been critical voices that opposed this kind of thinking with diagrams at least since Charles Darwin, it is especially from the interwar period that the ‘racial trees’ came under critique as relying on a faulty understanding of genetics and evolution, and as racist – a critique that became louder in the aftermath of World War II. The debates once again related to larger politics, and also up until this time, the anthropological family tree can be connected to the pedigrees developed and used in eugenics research, most of all in studies of ‘racial mixing’.
Ultrasound-guided quadratus lumborum block in sheep: A cadaveric study
Authors: Mattaliano Giorgio , Verdier Natali , Klonner Moriz E , Kyllar Michal , Kau-Strebinger Silvio , Otero Pablo E
Published: July 2024

This prospective anatomical study aimed to establish an ultrasound-guided technique to the quadratus lumborum (QL) plane in sheep cadavers. Thirteen cadavers, weighing less than 117 kg, were included. In phase 1, one cadaver underwent dissection and two cadavers underwent 3D computed tomographic reconstruction for anatomical evaluation of the thoracolumbar region. In phase 2, two cadavers were used to compare two ultrasound techniques to the QL plane: lateral to the QL muscle with a transversal approach (LQL) and transmuscular between QL and psoas muscles with a longitudinal approach (TQL). For LQL, the reference was the first lumbar transverse process, whereas for TQL, it was the intertransverse region between the first and second lumbar vertebrae. The needle was advanced in-plane towards the specific target for each technique and a total of four injections were performed using 0.4 ml kg−1 of a dye-lidocaine solution. In phase 3, 10 cadavers received bilateral LQL injections ( n = 20). All cadavers were then dissected to evaluate spread of dye. In phase 2, following LQL injections, no dye was observed in undesired locations; however, the dye was noted in the retroperitoneal space (1/2) after TQL injections. In phase 3, the 13th thoracic, first, second, third lumbar nerves, and sympathetic trunk segments were stained in 80%, 95%, 100%, 45% and 35% of the injections, respectively. In conclusion, the LQL technique was feasible, allowing staining of the spinal nerves innervating the cranial abdomen in sheep cadavers. Further studies in live animals are warranted.
The North-South divide and everything that gets left out in-between: conceptualizing Central and Eastern Europe to explain its positioning on climate change
Authors: Drieschova Alena
Published: July 2024

The North-South divide forms the central axis along which scholars study the contemporary global order. Yet many countries fall in-between the cracks of a world divided into core and periphery. This paper develops a structural account to understand the position of countries in this space of in-betweenness. The focus is on Central and Eastern Europe. I draw on already existing scholarship on liminalities, the varieties of capitalism and transition studies to argue that a liminal identity of in-betweenness goes hand-in-glove with a domestic logic of transitioning, as the state seeks to move somewhere else. Furthermore transitioning dynamics position the state in the semi-periphery, as the transition to the civilizational core requires capital and know-how from abroad. The resulting semi-peripheral position further underlines the liminal identity. The paper uses this apparatus to understand why CEE countries are typically climate change laggards within the EU. Their continuing liminal identity results in frustration over and resistance against the schooling tendencies of Brussels and Western European capitals. The dynamics of a sense of imposition of climate change mitigation policies stem from CEE’s liminal positioning as apprentices, but the reasons for the perceived alienness of such policies are located in domestic societal dynamics, and CEE countries’ economic structure. The specific political structures of communism and the communist transition have strengthened particularistic personal ties of friendship and family between individuals and the localities they live in, while simultaneously weakening general and abstract conceptions of the public good. Accordingly initiatives for preserving specific localities can be strong, but conceptions of protecting an abstract, global climate, are not well developed. Additionally, the material costs of protecting the climate are higher in post-communist economies due to their comparative advantage in resource and labour intensive industries, their reliance on foreign capital, and a lack of domestic innovations.
Organizing in the Folding of Time: Shaping organizational change trajectories at turning points
Authors: Hernes Tor , Feuls Miriam
Published: July 2024

Organizational change research has shown how change processes in organizations connect sequentially to form a trajectory of change through time; however, research has yet to offer an understanding of how ongoing organizational change integrates more distant change processes across time to shape the organization’s long-term trajectory. To better understand the dynamics between ongoing change and the shaping of trajectories, we refer to change processes as turning points, arguing that turning points are immanent to – and formative of – trajectories. We define turning points in terms of their definitional, directional and curvilinear features, which we relate to the shaping of organizational change trajectories. Drawing on Deleuze’s concept of the fold and the distinction between actual and singular events, we theorize how actors connect past and future turning points of the trajectory as singular events into enacting the actual events of the current turning point. Inspired by Deleuze’s conception of the fold, we then develop a theoretical framework for the shaping of organizational change trajectories at turning points. This framework contributes to current change research by explaining how ongoing change processes give shape to trajectories of change that subsequently shape the ongoing process of change. It also contributes to the literature by proposing a more dynamic and central role of continuity in change. Finally, our use of Deleuze’s concept of the fold enables us to suggest how theorizing actors’ movement through time may extend current process views of time.
How Algorithms Promote Self-Radicalization: Audit of TikTok’s Algorithm Using a Reverse Engineering Method
Authors: Shin Donghee , Jitkajornwanich Kulsawasd
Published: July 2024

Algorithmic radicalization is the idea that algorithms used by social media platforms push people down digital “rabbit holes” by framing personal online activity. Algorithms control what people see and when they see it and learn from their past activities. As such, people gradually and subconsciously adopt the ideas presented to them by the rabbit hole down which they have been pushed. In this study, TikTok’s role in fostering radicalized ideology is examined to offer a critical analysis of the state of radicalism and extremism on platforms. This study conducted an algorithm audit of the role of radicalizing information in social media by examining how TikTok’s algorithms are being used to radicalize, polarize, and spread extremism and societal instability. The results revealed that the pathways through which users access far-right content are manifold and that a large portion of the content can be ascribed to platform recommendations through radicalization pipelines. Algorithms are not simple tools that offer personalized services but rather contributors to radicalism, societal violence, and polarization. Such personalization processes have been instrumental in how artificial intelligence (AI) has been deployed, designed, and used to the detrimental outcomes that it has generated. Thus, the generation and adoption of extreme content on TikTok are, by and large, not only a reflection of user inputs and interactions with the platform but also the platform’s ability to slot users into specific categories and reinforce their ideas.
More Money, More Problems? Implications of Excess Cash in Nonprofit Hospitals
Authors: Gupta Anubhav
Published: July 2024

This study examines the implications of excess cash in nonprofit hospitals. Using a nationally representative sample of hospitals, I benchmark nonprofit cash holdings against for-profit cash holdings, and find that nonprofit hospitals hold significantly more cash. I consider three potential uses of excess cash in nonprofit hospitals: (a) investment in fixed assets, (b) increased charity care provision, and (c) higher executive compensation. I find that excess cash is associated with investments in fixed assets but not with increased charity care provision. Moreover, I find a positive relationship between excess cash and growth in CEO compensation. The results suggest that while nonprofit hospitals may accumulate cash to finance fixed assets, some of the accumulated cash may be diverted toward executive compensation. Overall, concerns regarding agency problems in nonprofit organizations may not be unfounded.
CCL3 Promotes Cutaneous Wound Healing Through Recruiting Macrophages in Mice
Authors: Shi Wanwan , Li Xunsheng , Wang Zhen , Li Chenguang , Wang Datao , Li Chunyi
Published: July 2024

Wound healing is a complex process, which involves three stages: inflammation, proliferation, and remodeling. Inflammation is the first step; thus, immune factors play an important regulatory role in wound healing. In this study, we focused on a chemokine, C-C motif chemokine ligand 3 (CCL3), which is often upregulated for expression during wound healing. We compared cutaneous wound healing at the histological, morphological, and molecular levels in the presence and absence of CCL3. The results showed that the wound healing rate in the wild-type and CCL3-/- + CCL3 mice was faster than that of CCL3-/- mice ( P < 0.01), and application of CCL3 to wounds increased the healing rate. In the process of wound healing, the degree of reepithelialization and the rate of collagen deposition in the wound of CCL3-/- mice were significantly lower than those of wild-type mice ( P < 0.01). The number of macrophages and the expression levels of tumor necrosis factor(TNF)-α and transforming growth factor (TGF)-β1 in the wounds of wild-type mice were much higher than those of the CCL3-/- mice. Removal of macrophages and CCL3-/- mice share similar phenotypes. Therefore, we infer that the wound healing requires the participation of macrophages, and CCL3 may play an important regulatory role through recruiting macrophages to the wound sites.
Association of nutritional intake with clinical and imaging activity in pediatric multiple sclerosis
Authors: Mohan Sonam D , Peterson Skyler , Brenton J Nicholas , Carmichael Suzan L , Virupakshaiah Akash , Rodriguez Moses , Tillema Jan-Mendelt , Mar Soe , Rensel Mary R , Abrams Aaron , Chitnis Tanuja , Benson Leslie , Gorman Mark , Lotze Tim , Shukla Nikita , Graves Jennifer , Aaen Gregory , Casper T Charles , Waubant Emmanuelle
Published: July 2024

Background:

Understanding nutrition’s role in multiple sclerosis (MS) can guide recommendations and intervention-based studies.

Objective:

Evaluate the association between nutrition and pediatric-onset MS outcomes.

Methods:

Prospective longitudinal multicenter study conducted as part of the US Network of Pediatric MS centers. Predictors were collected using a food screener estimating intake of various dietary food groups (e.g. dairy and fruits) and additional calculated indices (e.g. Healthy Eating Index (HEI)). Outcomes included time-from-enrollment to clinical relapse, new magnetic resonance imaging (MRI) T2 lesions, and Expanded Disability Status Scale (EDSS) increase.

Results:

353 children with MS were enrolled (mean ± SD age 15.4 ± 2.9, follow-up 3.9 ± 2.6 years). Multivariable analysis demonstrated that increased dairy by 50% of recommended intake was associated with increased relapse risk by 41% (adjusted hazard ratio (HR) 1.41, 95% CI 1.07–1.86), and risk of T2 progression by 40% (1.40, 1.12–1.74). Increased intake of fruit or vegetable above recommended, and every five-point HEI increase decreased relapse risk by 25% (0.75, 0.60–0.95), 45% (0.55, 0.32–0.96), and 15% (0.84, 0.74–0.96), respectively. No associations were found with EDSS.

Conclusion:

This work supports the influence of dietary intake on MS course, particularly with dairy intake. Future prospective study is required to establish causation.
Flexural performance of the negative moment region in bonded steel-wire-rope-strengthened reinforced concrete T-beams at different prestressing levels
Authors: Haryanto Yanuar , Sudibyo Gathot Heri , Nugroho Laurencius , Hu Hsuan-Teh , Han Ay Lie , Hsiao Fu-Pei , Widyaningrum Arnie , Susetyo Yudi
Published: July 2024

This work examines the performance of reinforced concrete (RC) beams strengthened using bonded steel wire rope (SWR) at various prestressing levels. The strengthening approach has, however, been applied to the flexural strengthening of RC T-beams in the negative moment region, in order to determine its advantages. For this purpose, four RC T-beams were fabricated and tested under monotonic four-point bending: one control beam (S00), one beam strengthened with non-prestressed SWR (S20), and two beams strengthened with SWR (prestressed at 10% and 20% of their ultimate tensile strength: S21 and S22). The results indicate that the strengthened beams exhibit higher load-carrying capacities. Specifically, the cracking load, yield load, and ultimate load of S20, S21, and S22 increase by 10%–30%, 30%–50%, and 50%–90%, respectively, compared to S00. Additionally, there is an improvement in stiffness and energy absorption capacity. However, these strategies may have a dual effect on the specimens, resulting in a reduction in their ductility index. Finally, the tested beams were replicated using a three-dimensional finite element model, which has proved effective in predicting the behavior of such structures and, therefore, was found to be appropriate for use in future studies.
The Importance and Limitation of Epistemic Norms in Political Theory
Authors: Erman Eva , Möller Niklas
Published: July 2024

One recent debate in political theory centers on the question of whether there is a distinctively political normativity. According to an influential view, there is a distinctive set of norms that applies specifically to political actions and decisions, which are not grounded in moral normativity. On one version of this non-moral view, political theory is grounded in epistemic normativity (and thus epistemic norms). Theorists identifying as “radical realists” insist that political theorists do not need any moral normativity (and thus moral norms), because epistemic normativity may provide action-guidance for political theory. In this article, we take our point of departure in a critical analysis of this epistemic version of the non-moral view, with the overall aim of analyzing the importance and limitation of epistemic norms in political theory. We argue that epistemic norms are necessary—since a political theory should not rely on empirical falsities—but not sufficient for a successful account in the political domain. Two claims are made: moral norms are essential in the process of political theorizing, both in the form of pre-epistemic norms and in the form of post-epistemic norms. More specifically, we contend, first, that we need moral norms to identify and justify which practices to study when conducting political theorizing, and second, that we need moral norms to tell us how to act in light of our investigation of warranted and unwarranted beliefs.
State of the Data Summary: National School Health Data Set: Every Student Counts! 2023–2024 School Year
Authors: Stanislo Kimberly J.
Published: July 2024

The National School Health Data Set: Every Student Counts! ( ESC!) is NASN’s data initiative focusing on building data capacity for school nurses, a uniform data set with standardized definitions, and promoting data infrastructure including school nurse access to electronic documentation, interoperability of educational systems and school health records, and build partnerships to increase data collection, storage, retrievable, and utilization. Each year since 2018, states have submitted data to NASN for inclusion in the National School Health Data Set. Participation is built on a tiered programing model to include school nurses at the school, state, and national level. Every state has identified a State Data Coordinator (SDC) who serves as a liaison to NASN to support ESC! but also provides support to school nurses in their state. This article provides an overview of the ESC! data initiative for the 2023–2024 school year, which includes the data from the 2022–2023 school year.
The Impact of Covid-19 on Earnings Management: Empirical Evidence from Vietnam
Authors: Dang Anh Tuan , Khanh Dung Nguyen Ngoc
Published: July 2024

Earnings management (EM) is the practice of adjusting profits or earnings on financial statements at the discretion of the management. This study undertook a regression analysis of the earnings management model, with data from 300 non-financial listed companies on the Vietnamese stock exchange during the period 2016 to 2021 to determine whether the COVID-19 pandemic has had an impact on earnings management. To estimate earnings management, compared with the original model, we add the leverage variable (LV) was used as earnings management proxy in this research. The results show that the COVID-19 pandemic positively and significantly impact the earnings management of non-financial listed companies. Moreover, it was found that managers are more involved in real earnings management than accrual earnings management, implying that financially distressed firms need to reassure investors to raise more capital during the pandemic. However, there is no evidence of a trade-off between these two techniques during the pandemic compared to before the pandemic. Additionally, this study provides evidence that the model by Roychowdhury, following Cohen et al., and the performance-matched model by Jones, following Kothari et al. are more suitable for detecting EM behavior when factoring in the financial leverage (LV) variable for firms with a high debt ratio in developing countries.

JEL Classification: H8, M42
Morphology and ITS sequences provide insights into the phylogeny of Tongoloa (Apiaceae) from China
Authors: Gui Lingjian , Peng Chang , Yu Liying , Liu Lijia , Wei Shugen , Yan Zhigang , Zhang Xiaomei , Zhou Songdong , He Xingjin
Published: July 2024

Background

Tongoloa is a genus comprising approximately 20 species, primarily distributed in the mountainous regions of southwest China. The insufficiency of specimen materials and morphological similarities among species render it a taxonomically challenging genus within the Apiaceae family. To elucidate the phylogenetic relationships and taxonomy of Chinese Tongoloa, this study utilized a total of 115 nrITS sequences, including 47 recently obtained sequences, for phylogenetic reconstruction.

Results

Phylogenetic relationships reconstructed from ITS sequences indicate that the East Asia Clade and the Komarovia Clade are sister groups, and Tongoloa belongs to the East Asia Clade. Species of Tongoloa are subdivided into 3 distinct groups, all sharing similar fruit morphologies and are clearly differentiated from related taxa. Several Tongoloa-like members classified under other genera are interpreted to be closely related to Tongoloa. Morphological and molecular data indicate that Tongoloa, Sinolimprichtia subclade and Chinese Trachydium subclade are separate yet genetically contiguous taxa. It is confirmed that Tongoloa zhongdianensis belongs to the Hymenidium Clade, while Sinocarum is classified within the Acronema Clade. Two new taxa are found in the Hengduan Mountains.

Conclusion

Tongoloa is a genus within the East Asia Clade of Apiaceae, and the phylogeny reconstructed based on ITS sequences divides it into 3 main groups. By integrating fruit morphology and molecular phylogenetic analyses, we preliminary clarified the intricate taxonomic relationships among Tongoloa and related taxa. These results provide valuable opportunities for a deeper understanding of the phylogeny of Tongoloa.
Perinatal maternal factors influencing postpartum feeding practices at six weeks
Authors: Wang Yanchi , Mao Kai , Chu Minjie , Lu Xiaopeng
Published: July 2024

Objective

Despite the World Health Organization’s recommendation of exclusive breastfeeding for the initial 6 months, breastfeeding rates decline within the first 6 weeks after delivery. This study aimed to (1) investigate the breastfeeding rate at 6 weeks postpartum and (2) explore the influence of perinatal factors on feeding patterns at 6 weeks postpartum.

Method

A total of 635 participants were enrolled from February to August 2023 at outpatient clinics in three tertiary hospitals in Nantong City. Variables were collected through questionnaires during the third trimester of pregnancy, including demographic information, pregnancy stress, anxiety, depression, sleep, and resilience. At 6 weeks postpartum, information regarding feeding patterns, delivery and postpartum situations, postpartum stress, anxiety, depression, sleep, and resilience was gathered. Initial single-factor analyses were conducted using feeding pattern as the dependent variable, and variables with significance were chosen as independent variables. The disordered multi-classification logistic regression model was then established using the stepwise forward method.

Results

Within the first 6 weeks, 35.28% (224/635) of postpartum women exclusively breastfed their infants. Factors influencing exclusive breastfeeding and formula feeding at 6 weeks postpartum included breast pain, sleep quality, mental resilience, difference between postpartum and late pregnancy anxiety, insufficient milk supply, and maternal herself caring for the infant (P < 0.05). Factors influencing the transition from exclusive to partial breastfeeding were insufficient milk supply and maternal herself caring for the infant (P < 0.05).

Conclusion

The study reveals a relative low rate of exclusive breastfeeding in China’s first 6 weeks postpartum, along with a comparison of perinatal factors affecting three different feeding patterns. Our findings may contribute additional evidence to the association between perinatal factors and feeding patterns. This study guides healthcare professionals in developing strategies to promote exclusive breastfeeding and improve personalized counseling for exclusive breastfeeding and mental health.
Comparison of factors affecting Turkish citizens’ search for online health information before and during the COVID-19 pandemic
Authors: Alkan Ömer , Küçükoğlu Uğur , Ünver Şeyda
Published: July 2024

Background

Health information consumers can acquire knowledge regarding health problems, combat health problems, make health-related decisions, and change their behaviour by conducting health information searches. This study aims to identify the sociodemographic and economic factors affecting individuals’ search for health information on the internet before and during COVID-19.

Methods

In this study, micro data sets of the Household Information Technologies (IT) Usage Survey conducted by the Turkish Statistical Institute in 2018 and 2021 were used. The binary logistic regression analysis was also used in the study.

Results

It was determined that age, gender, education level, occupation, social media use, searching for information about goods and services, internet banking use, e-government use, having a desktop computer, having a tablet computer, and region variables were associated with the status of searching for health information on the internet during the COVID-19 period.

Conclusion

The main reasons for the increase in health information searches during the COVID-19 epidemic can be attributed to several key factors, such as society’s need for information and meeting its need for information, access to up-to-date health data and increased trust in official sources. The study’s findings serve as a valuable resource for health service providers and information sources attempting to identify the health information-seeking behaviour of the public and to meet their needs in this context.
Traditional Chinese medicine for sjögren’s syndrome: a national survey of attitudes and perceptions among Chinese patients and rheumatologists
Authors: Liu Ruihua , Guo Zilin , Xu Haodong , He Jing , Wang Bei , Jin Yuebo , Zhao Ziying , Tang Xiaopo , Zhou Xinyao
Published: July 2024

Background

This study explored similarities and differences among Chinese patients and rheumatologists in their attitudes towards and perceptions of traditional Chinese medicine (TCM) for Sjögren’s syndrome (SS), including analyzing factors that influenced their decision making.

Methods

An anonymous questionnaire was used to conduct a multicenter survey among patients with SS at three tertiary care medical centers in Beijing and among rheumatology clinicians at several hospitals across China. Results were analyzed using descriptive statistics.

Results

There were 942 valid questionnaires from patients from 31 provinces and cities in China, with a male-to-female ratio of approximately 1:14, a mean age of 48.81 years, and a median disease duration of 7 (4, 10) years. There were 320 valid questionnaires from rheumatologists, covering 30 provinces and cities in China, with a male-to-female ratio of approximately 0.87:1, a mean age of 48 years, and a median work duration of 10.5 (6, 15) years. The rheumatologists treated a median of 15 (11, 50) SS cases per month, and the median proportion of SS to all rheumatic diseases was 6.66% (6–10%). Many patients believed TCM could cure the root of the disease, and the most expected TCM therapies were TCM patent prescriptions and medicinal teas. Conversely, rheumatologists placed high value on the efficacy of TCM, and most commonly prescribed Chinese herbal decoctions. Most doctor-patient groups were positive about TCM treatment, citing the low side effects as the major advantage. Regression analysis showed that for patients over 40 years old with a course of disease > 4 years, the probability of using TCM has increased by 1–6 times; the probability of recommending TCM in clinical work of doctors who have worked for more than 15 years, TCM and integrated traditional Chinese and western medicine has increased 1–2 times.

Conclusions

TCM has become widely accepted and earned attention from doctor-patient groups, especially among older patients and experienced rheumatologists. However, negative prejudices and absence of accurate information about TCM treatments and SS itself require improvement. The contradiction between TCM dosage form and efficacy is a major problem, and patient demand for convenient and efficient TCM patent preparations suggests future work should focus on developing TCM patent preparations with clear compositions and mechanisms.
Unspoken expectations and situational participation: a qualitative study exploring the instantiation of next of kin involvement within the trust model
Authors: Slåtsveen Ruth-Ellen , Wibe Torunn , Halvorsrud Liv , Lund Anne
Published: July 2024

Background

Demographic changes, such as an increase in older adults, present a challenge to the healthcare service’s current capacity. Moreover, the need for healthcare personnel is rising, while the availability of labour is dwindling, leading to a potential workforce shortage. To address some of these challenges, enhanced collaboration between home-based healthcare frontline workers, service users, and next of kin is a necessity. The trust model is an organisational model where home-based healthcare services are organised into smaller interdisciplinary teams aiming to tailor the services in collaboration with service-users and their next of kin’. This study explores how the next of kin and frontline workers perceive and perform involvement in making decisions regarding tailoring the services for the users of home-based healthcare services organised after the trust model.

Methods

Four in-depth interviews and 32 observations were conducted, and thematic analysis was employed to identify meaningful patterns across the datasets.

Results

The results are presented as two themes: (i) unspoken expectations and (ii) situational participation. The results highlight the complex nature of next-of-kin involvement and shared decision making, raising questions about meeting expectations, evaluating available resources, and developing sustainable involvement processes.

Conclusion

This study indicates that despite of an interdisciplinary organisational model aiming for shared decision making as the trust model, the involvement of next of kin continues to be a challenge for frontline workers in home-based healthcare services. It also points to the importance of transparent communication and how it is deemed essential for clarifying implicit expectations.
Insight into the role of Streptococcus suis zinc metalloprotease C from the new serotype causing meningitis in piglets
Authors: Gu Qibing , He Peijuan , Bai Qiankun , Zhong Xiaojun , Zhang Yue , Ma Jiale , Yao Huochun , Pan Zihao
Published: July 2024

Streptococcus suis (S. suis) is an important gram-positive pathogen and an emerging zoonotic pathogen that causes meningitis in swine and humans. Although several virulence factors have been characterized in S. suis, the underlying mechanisms of pathogenesis are not fully understood. In this study, we identified Zinc metalloproteinase C (ZmpC) probably as a critical virulence factor widely distributed in S. suis strains. ZmpC was identified as a critical facilitator in the development of bacterial meningitis, as evidenced by the detection of increased expression of TNF-α, IL-8, and matrix metalloprotease 9 (MMP-9). Subcellular localization analysis further revealed that ZmpC was localized to the cell wall surface and gelatin zymography analysis showed that ZmpC could cleave human MMP-9. Mice challenge demonstrated that ZmpC provided protection against S. suis CZ130302 (serotype Chz) and ZY05719 (serotype 2) infection. In conclusion, these results reveal that ZmpC plays an important role in promoting CZ130302 to cause mouse meningitis and may be a potential candidate for a S. suis CZ130302 vaccine.
Identification of plasma miR-4505, miR-4743-5p and miR-4750-3p as novel diagnostic biomarkers for coronary artery disease in patients with type 2 diabetes mellitus: a case-control study
Authors: Szydełko Joanna , Czop Marcin , Petniak Alicja , Lenart-Lipińska Monika , Kocki Janusz , Zapolski Tomasz , Matyjaszek-Matuszek Beata
Published: July 2024

Background

Type 2 diabetes mellitus (T2DM) and coronary artery disease (CAD) are commonly coexisting clinical entities with still growing incidence worldwide. Recently, circulating microRNAs (miRNAs) have emerged as novel molecular players in cardiometabolic diseases. This study aimed to identify a specific miRNA signature as a candidate biomarker for CAD in T2DM and to delineate potential miRNA-dependent mechanisms contributing to diabetic atherosclerosis.

Methods

A total of 38 plasma samples from T2DM patients with and without CAD, CAD patients and healthy controls were collected for expression profiling of 2,578 miRNAs using microarrays. To investigate the regulatory role of differentially expressed (DE)-miRNA target genes, functional annotation and pathway enrichment analyses were performed utilizing multiple bioinformatics tools. Then, protein-protein interaction networks were established leveraging the STRING database in Cytoscape software, followed by cluster analysis and hub gene identification. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was carried out for microarray data validation in the larger replication cohort of 94 participants. Receiver operating characteristic analysis was applied to evaluate the diagnostic values of miRNAs. Multivariate logistic regression analysis was used to develop miRNA-based diagnostic models.

Results

In the discovery stage, overexpression of hsa-miR-4505, hsa-miR-4743-5p, hsa-miR-6846-5p, and down-regulation of hsa-miR-3613-3p, hsa-miR-4668-5p, hsa-miR-4706, hsa-miR-6511b-5p, hsa-miR-6750-5p, hsa-miR-4750-3p, hsa-miR-320e, hsa-miR-4717-3p, hsa-miR-7850-5p were detected in T2DM-CAD patients. The DE-miRNA target genes were significantly enriched in calcium ion binding, regulation of actin cytoskeleton, and gene expression. hsa-miR-4505, hsa-miR-4743-5p, and hsa-miR-4750-3p were found to be involved in fatty acid metabolism, leukocyte transendothelial migration, and neurotrophin signaling pathway. Dysregulation of hsa-miR-4505, hsa-miR-4743-5p, and hsa-miR-4750-3p in T2DM-CAD patients compared with T2DM subjects and controls (all p < 0.001) was further confirmed by RT-qPCR. All validated miRNAs demonstrated good discriminatory values for T2DM-CAD (AUC = 0.833–0.876). The best performance in detecting CAD in T2DM was achieved for a combination of three miRNAs (AUC = 0.959, 100% sensitivity, 86.67% specificity).

Conclusions

Our study revealed a unique profile of plasma-derived miRNAs in T2DM patients with CAD. Potential miRNA-regulated pathways were also identified, exploring the underlying pathogenesis of CAD in T2DM. We developed a specific three-miRNA panel of hsa-miR-4505, hsa-miR-4743-5p and hsa-miR-4750-3p, that could serve as a novel non-invasive biomarker for CAD in patients with T2DM.
Fast-track surgery with three-port versus conventional perioperative management of bladder cancer associated laparoscopic radical cystectomy and Ileal conduit diversion: Chinese experience
Authors: Lin Guangzheng , Wang Xin , Ma Jiaxing , Sun Wei , Han Chengxiang , Tang Liang
Published: July 2024

Objective

This study seeks to explore the impact of fast track surgery (FTS) with three-port in patients treated with laparoscopic radical cystectomy and ileal conduit on postoperative recovery, hospital stay and the complications.

Methods

This retrospective study analyzed 230 patients with invasive bladder cancer who underwent laparoscopic radical cystectomy at the Second Hospital of Anhui Medical University between December 2011 to January 2023. 50 patients received conventional surgery (CS) and 180 patients received FTS with three-port. Patients were assessed for time to normal diet consumption, time to passing first flatus, number of postoperative recovery days and complications. Trends of serum C-reactive protein levels were monitored preoperatively and on postoperative days 1, 3 and 7.

Results

Patients who underwent FTS with three-port had a shorter duration to first flatus (P < 0.05). And number of postoperative hospital days and the length of hospital stay were notably shorter in contrast to the CS group (P < 0.05). Serum CRP levels on postoperative day 7 were markedly reduced in those of the FTS group compared to the CS group (P < 0.05). Those of the CS group experienced more frequent rates of complications compared to those of the FTS with three-port group (P < 0.05).

Conclusion

Our findings demonstrate that the FTS with three-port program hastens postoperative recovery and reduces duration of hospital stay. It is safer and more effective than the CS program in the Chinese population undergoing laparoscopic radical cystectomy.
Italian and Middle Eastern adherence to Mediterranean diet in relation to Body Mass Index and non-communicable diseases: nutritional adequacy of simulated weekly food plans
Authors: Luconi Ester , Tosi Martina , Boracchi Patrizia , Colonna Ilaria , Rappocciolo Emilia , Ferraretto Anita , Lorenzini Erna C.
Published: July 2024

Background

The Mediterranean diet (MD), known to prevent obesity, overweight and the related non communicable diseases (NCD), is based on typical dishes, foods and on a common cultural milieu. Although MD is the basis of dietary guidelines, the prevalence of obesity, overweight and NCD, is increasing both in Western regions, and even more in Middle Eastern regions (MER). This study aimed to analyze (i) the impact of different levels of adherence to the MD, in Italy and MER, on body mass index (BMI) (ii) the bromatological composition of a simulated 7-days food plan (7-DFP) based on Italian or MER typical meals, following MD criteria and the Italian or MER food base dietary guideline; (iii) the optimization of nutrients impacting on NCD.

Methods

The 7-DFPs were implemented using a dietary software. The association between adherence to MD and BMI was evaluated by pooled estimated ORs (with 95% confidence intervals and p-values). Pooled measures were obtained by the methods appropriate for meta-analysis. The different food-based guidelines have been compared.

Results

The pooled ORs of obese status comparing medium vs. high adherence to MD were: 1.19 (95% C.I.: 0.99; 1.42, p-value = 0.062) and 1.12 (95% C.I.: 0.90; 1.38, p-value = 0.311) for MER and Italy respectively. For the comparison of low vs. high adherence, the pooled ORs were 1.05 (95% C.I.: 0.88; 1.24, p-value = 0.598) for MER, and 1.20 (95% C.I.: 1.02; 1.41, p-value = 0.031) for Italy when outliers are removed. High adherence to the MD resulted as potential protective factor against obesity. In MER 7-DFP: total fats is higher (34.5 E%) vs. Italian 7-DFP (29.4 E%); EPA (20 mg) and DHA (40 mg) are lower than recommended (200 mg each); sugars (12.6 E%) are higher than recommended (< 10 E%). Calcium, Zinc, and vitamin D do not reach target values in both 7-DFPs.

Conclusion

This study highlights that, even when 7-DFPs follow MD and refer to nutrient needs, it is necessary to verify nutrient excesses or deficits impacting on NCD. High MD adherence is protective toward NCDs. MD principles, and energy balance should be communicated according to socioeconomic and educational levels.
Targeting tumor-infiltrating CCR8+ regulatory T cells induces antitumor immunity through functional restoration of CD4+ Tconvs and CD8+ T cells in colorectal cancer
Authors: Chen Qian , Shen Meiying , Yan Min , Han Xiaojian , Mu Song , Li Ya , Li Luo , Wang Yingming , Li Shenglong , Li Tingting , Wang Yingying , Wang Wang , Wei Zhengqiang , Hu Chao , Jin Aishun
Published: July 2024

Background

Chemokine (C-C motif) receptor 8 (CCR8) is a chemokine receptor selectively expressed on tumor-infiltrating regulatory T cells (Tregs). Strong immunosuppression mediated by CCR8+ Tregs observed in breast and lung malignancies suggest for their functional significance in cancer therapy. To date, detailed characterization of tumor-infiltrating CCR8+ Tregs cells in colorectal cancer (CRC) is limited.

Methods

To study the presence and functional involvement of CCR8+ Tregs in CRC, we analyzed the proportions of CCR8-expressing T cells in different T cell subsets in tumor and adjacent normal tissues and peripheral blood mononuclear cells (PBMCs) from CRC patients by Flow cytometry. Also, we compared the distribution of CCR8+ T cells in malignant tissues and peripheral lymphoid organs from a subcutaneous CRC murine model. Bioinformatic analysis was performed to address the significance of CCR8 expression levels in CRC prognosis, immune regulatory gene expression profiles and potential molecular mechanisms associated with CCR8+ Tregs in CRC tumors. Further, we administrated an anti-CCR8 monoclonal antibody to CT26 tumor-bearing mice and examined the antitumor activity of CCR8-targeted therapy both in vivo and in an ex vivo confirmative model.

Results

Here, we showed that Tregs was predominantly presented in the tumors of CRC patients (13.4 ± 5.8, p < 0.0001) and the CRC subcutaneous murine model (35.0 ± 2.6, p < 0.0001). CCR8 was found to be preferentially expressed on these tumor-infiltrating Tregs (CRC patients: 63.6 ± 16.0, p < 0.0001; CRC murine model: 65.3 ± 9.5, p < 0.0001), which correlated with poor survival. We found that majority of the CCR8+ Tregs expressed activation markers and exhibited strong suppressive functions. Treatment with anti-CCR8 antibody hampered the growth of subcutaneous CRC tumor through effectively restoring the anti-tumor immunity of CD4+ conventional T cells (CD4+ Tconvs) and CD8+ T cells, which was confirmed in the ex vivo examinations.

Conclusions

Collectively, these findings illustrate the importance of CCR8+ Tregs for an immunosuppressive microenvironment in CRC tumors by functional inhibition of CD4+ Tconvs and CD8+ T cells, and suggest for the applicable value of CCR8-targeted therapy for CRC.
Triglyceride-glucose index and glycemic dynamics in pancreatic ductal adenocarcinoma: implications for disease progression and prognosis
Authors: Song Yunda , Jiang Lingmin , Han Yuanxia , Zhang Subo , Li Shengping
Published: July 2024

Background

To elucidate the relationship between the triglyceride-glycemic index (TyG) and clinical characteristics of pancreatic ductal adenocarcinoma (PDAC).

Methods

A total of 1,594 individuals diagnosed with pancreatic and periampullary neoplasms were categorized into four groups: PDAC-early (n = 403), locally advanced PDAC (LAPC, n = 315), PDAC-late with distant metastasis (n = 371), and other tumor types (n = 505). TyG-high was defined as a TyG index greater than 8.81 in males and 8.73 in females.

Results

The prevalence of TyG-high status was highest in PDAC-early (68.48%), followed by LAPC (53.33%), and lowest in PDAC-late (44.47%). TyG-high status significantly predicted worse PDAC prognosis (P = 0.0166), particularly in PDAC-late (P = 0.0420). Despite similar blood glucose levels across PDAC groups (P = 0.897), PDAC-early patients showed significantly higher rates of glycemic disturbances (56.33% vs. 32.28%) and TyG-high status (68.48% vs. 47.13%) compared to those with other tumors. Progressive increases in glycemic disturbances and TyG-high status were observed from benign to pre-malignant lesions and PDAC-early. PDAC-early patients at the pancreatic head exhibited higher rates of glycemic disturbances (58.12% vs. 33.33%, P < 0.0001), larger pancreatic duct diameters (0.4056 cm vs. 0.3398 cm, P = 0.0043), and poorer prognosis compared to periampullary cancers, although the TyG-high rate and body mass index were similar.

Conclusion

The TyG index exhibits a complex association with PDAC stages, profoundly shaping glycemic profiles. At the initial stages of PDAC, a notable elevation in TyG-high status and glycemic disturbances is observed. However, in advanced PDAC, while the TyG-high rate diminishes, abnormal glucose levels persist.
Viral metagenomics combined with non-targeted serum metabolomics reveals the role of enteroviruses in a mouse model of coronary heart disease
Authors: Li Xiang , Liu Sihong , Wu Haonan , Li Bing , Li Yingying , Li Ruoqi , Tang Danli , Zhang Huamin
Published: July 2024

Background

Coronary heart disease (CHD) is a common cardiovascular disease that is associated with altered gut microbiota. Enteroviruses, an essential component of the gut microbiome, may play an important role in disease progression. However, the relationship between enteroviruses and CHD remains unclear. The development of high-throughput sequencing technologies has facilitated research on the interconnections between viruses and disease-related metabolites.

Methods and results

Mice were fed a high-fat diet (CHD group) or chow diet (Sham group) for 12 weeks, and ligation of the left anterior descending coronary artery was performed at the end of week 8. After 4 weeks, all animals were euthanised. Subsequently, the animals were evaluated for basic haemato-biochemical parameters and cardiac function, and aorta staining was performed. Based on enteroviral metagenomics and serum UPLC–MS/MS metabolomics analyses, we evaluated the association between enteroviral groups and serum metabolites of CHD mouse model. A high-fat diet and coronary ligation enabled the establishment of the CHD mouse model. Notably, the enterovirus spectrum of the sham group was significantly different from that of the CHD group, with 24 viral communities of different family and species classification, such as Tsarbombavirus, Mingyongvirus, Claudivirus, and Firehammervirus, exhibiting significant differences. In addition, 731 Differential metabolites were detected in the serum of both groups of mice. Correlation network analysis revealed a close relationship between various metabolites related to lipid metabolism and different viruses, including Tsarbombavirus, Mingyongvirus, Claudivirus, and Firehammervirus.

Conclusions

An animal model of CHD, characterised by lipid disturbance and myocardial ischaemia, was established using a high-fat diet and ligation of the left anterior descending branch of the coronary artery. Tsarbombavirus, Firehammervirus, Mingyongvirus, and Claudivirus were associated with metabolites in the lipid metabolism pathway. The results indicate that Tsarbombavirus may be the main genus interacting with CHD-related metabolites in mice. Conclusively, the findings of our study provide novel insights into the potential relationship enterovirus groups and metabolites associated with CHD.