bluuebunny/crossref_metadata_2025 huggingface dataset:
Reassessment of Poststroke Dysphagia in Rehabilitation Facility Results in Reduction in Diet Restrictions
Authors: Pekacka-Egli Anna Maria , Kazmierski Radoslaw , Lutz Dietmar , Pekacka-Falkowska Katarzyna , Maszczyk Adam , Windisch Wolfram , Spielmanns Marc
Published: April 2021

Background: Dysphagia assessment in postacute stroke patients can decrease the incidence of complications like malnutrition, dehydration, and aspiration pneumonia. It also helps to avoid unnecessary diet restrictions. The aim of this study is to verify if regular reassessment of dysphagia would change the diet management of postacute stroke patients in rehabilitation settings. Methods: This single-center retrospective study included 63 patients referred to an inpatient neurological rehabilitation center between 2018–2019. A standardized clinical swallowing evaluation and Fiberoptic Endoscopic Evaluation of Swallowing (FEES) were performed. Diet level according to Functional Oral Intake Scale (FOIS) was evaluated. As the primary endpoint, the FOIS values based on diagnostic procedures were assessed at hospital discharge, rehabilitation admission, and after FEES. Results: 19 women (30%) and 44 men (70%), with a mean age of 75 y (SD ± 10.08), were enrolled. The intergroup ANOVA revealed significant differences (p < 0.001) between dietary prescriptions in an acute care setting and following clinical and endoscopic reassessment in the rehabilitation center. Diet recommendations changed in 41 of 63 (65%) enrolled patients (p < 0.001). Conclusion: Instrumental diagnostic by FEES during the early convalescence period of stroke patients leads to clinically relevant changes to diet restrictions and lower rates of pneumonia. Our findings underline the need for regular and qualitative dysphagia diagnostics in stroke patients participating in neurological rehabilitation.
Superior Orbicularis Oris Muscle Activity in Children Surgically Treated for Bilateral Complete Cleft Lip and Palate
Authors: Szyszka-Sommerfeld Liliana , Machoy Monika Elżbieta , Wilczyński Sławomir , Lipski Mariusz , Woźniak Krzysztof
Published: April 2021

The aim of this cross-sectional study was to evaluate the electromyographic activity of the superior orbicularis oris muscle both in children surgically treated for bilateral complete cleft lip and palate (BCCLP) as well as in subjects without BCCLP. The study comprised 77 children aged 6.6 to 12.5 years. All the patients with clefts had previously undergone lip and palate surgery. The upper lip electromyographic (EMG) assessments were made with a DAB-Bluetooth device (Zebris Medical GmbH, Germany) at rest, while swallowing saliva, protruding lips and compressing lips. EMG measurements were also made when the subjects produced phonemes /p/, /b/, and /m/ with the vowel /a/. The Mann-Whitney U test was applied to statistically analyze the EMG values. Significantly higher median upper lip EMG activity under working conditions such as swallowing saliva, lip compression, and production of the phoneme /p/ with the vowel /a/ was observed in patients with BCCLP compared to those without a cleft. The results of the study showed that the upper lip muscle activity increases in children with BCCLP when swallowing saliva, compressing lips and during some speech movement tasks. This may be important in the aspect of the effect of surgical lip repair on the craniofacial growth.
Oral Antiplatelet Therapy for Secondary Prevention of Non-Cardioembolic Ischemic Cerebrovascular Events
Authors: De Luca Leonardo , Bellettini Elisa , Di Maio Dario , Natale Enrico , Putini Rita Lucia , Anticoli Sabrina , Colivicchi Furio , Calabrò Paolo , Musumeci Francesco , Gabrielli Domenico
Published: April 2021

Stroke is the leading cause of disability and mortality worldwide. After an acute cerebrovascular ischemia, recurrent vascular events, including recurrent stroke or transient ischemic accidents (TIA), occur in around 20% of cases within the first 3 months. In order to minimize this percentage, antiplatelet therapy may play a key role in the management of non-cardioembolic cerebrovascular events. This review will focus on the current evidence of antiplatelet therapies most commonly discussed in practice guidelines and used in clinical practice for the treatment of stroke/TIA complications. The antiplatelet therapies most commonly used and discussed are as follows: aspirin, clopidogrel, and ticagrelor.
Abnormal Liver Function Tests and Long-Term Outcomes in Patients Discharged after Acute Heart Failure
Authors: Miyama Hiroshi , Shiraishi Yasuyuki , Kohsaka Shun , Goda Ayumi , Nishihata Yosuke , Nagatomo Yuji , Takei Makoto , Fukuda Keiichi , Kohno Takashi , Yoshikawa Tsutomu
Published: April 2021

Abnormal liver function tests (LFTs) are known to be associated with impaired clinical outcomes in heart failure (HF) patients. However, this implication varies with each single LFT panel. We aim to evaluate the long-term outcomes of acute HF (AHF) patients by assessing multiple LFT panels in combination. From a prospective multicenter registry in Japan, 1158 AHF patients who were successfully discharged were analyzed (mean age, 73.9 ± 13.5 years; men, 58%). LFTs (i.e., total bilirubin, aspartate aminotransferase or alanine aminotransferase, and alkaline phosphatase) at discharge were assessed; borderline and abnormal LFTs were defined as 1 and ≥2 parameter values above the normal range, respectively. The primary endpoint was composite of all-cause death or HF readmission. At the time of discharge, 28.7% and 8.6% of patients showed borderline and abnormal LFTs, respectively. There were 196 (16.9%) deaths and 298 (25.7%) HF readmissions during a median 12.4-month follow-up period. The abnormal LFTs group had a significantly higher risk of experiencing the composite outcome (adjusted hazard ratio: 1.51, 95% confidence interval: 1.08–2.12, p = 0.017), whereas the borderline LFTs group was not associated with higher risk of adverse events when referenced to the normal LFTs group. Among AHF patients, the combined elevation of ≥2 LFT panels at discharge was associated with long-term adverse outcomes.
Vertical Bite Rehabilitation of Severely Worn Dentitions with Direct Composite Restorations: Clinical Performance up to 11 Years
Authors: Tauböck Tobias T. , Schmidlin Patrick R. , Attin Thomas
Published: April 2021

Our aim was to evaluate the clinical performance of direct composite restorations placed in patients with severely worn dentitions at an increased vertical dimension of occlusion, after up to 11 years. One hundred and sixty-four teeth in 13 patients with severely worn dentitions had been reconstructed with either microhybrid (first cohort; n = 59) or nanofilled (second cohort; n = 105) composite restorations at increased vertical dimension of occlusion using a wax-up-based template-aided placement technique. From the dental records, information about repair and replacement of restorations was obtained. Patients were clinically examined after a mean follow-up time of 10.7 years (first cohort) or 5.2 years (second cohort) using United States Public Health Service (USPHS) criteria. Subjective patient satisfaction was also recorded using visual analogue scales (VAS). The overall quality of the restorations was good with predominantly ‘Alpha’ and ‘Bravo’ scores, respectively. Nanofilled composite showed less surface degradation and better margin qualities than microhybrid composite. Of the 59 restored teeth in the first cohort, 13 restorations showed unfavorable events after 10.7 years, of which ten could be repaired. In the second cohort, 23 of 105 restorations showed unfavorable events, which could all be repaired. VAS scores revealed high patient satisfaction with the treatment approach. In conclusion, direct composite restorations placed at an increased vertical dimension of occlusion show good clinical long-term performance in patients with severe tooth wear.
Emerging Approaches in Intravenous Moderate and Deep Sedation
Authors: Goudra Basavana , Mason Keira P.
Published: April 2021

Successful pharmacological innovations that have made a difference in daily practice are rare in the world of anesthesia and sedation. After many years of research, it seems that we finally have two new drug innovations that are likely to change the paradigm of moderate and deep sedation. These are oliceridine and remimazolam. Both have been in development for over a decade. Oliceridine was synthesized in a lab as an entirely new molecule. It is a biased μ- receptor agonist that acts preferentially on the G-protein pathway (which is responsible for analgesia). At least in lower doses, it has minimal effect on the beta-arrestin pathway, which is responsible for unwanted effects of μ-opioid receptor activation such as respiratory depression and gastrointestinal dysfunction. Like any other μ- receptor agonist, it produces appropriate dose-dependent analgesia. Remimazolam is structurally similar to midazolam; however, it has an additional ester linkage that delivers the kinetics of remifentanil. As a result, while pharmacodynamically identical to midazolam, remimazolam is metabolized by ester hydrolysis and subsequently its elimination is rapid and predictable. The present review discusses the two drugs in detail with a particular emphasis on their potential role in moderate and deep sedation.
Current Overview on Clinical Management of Chronic Constipation
Authors: Włodarczyk Jakub , Waśniewska Anna , Fichna Jakub , Dziki Adam , Dziki Łukasz , Włodarczyk Marcin
Published: April 2021

Constipation is one of the major gastrointestinal disorders diagnosed in clinical practice in Western countries. Almost 20% of population suffer from this disorder, which means constipation is a substantial utilization of healthcare. Pathophysiology of constipation is complex and multifactorial, where aspects like disturbance in colonic transit, genetic predisposition, lifestyle habits, psychological distress, and many others need to be taken into consideration. Diagnosis of constipation is troublesome and requires thorough accurate examination. A nonpharmacological approach, education of the patient about the importance of lifestyle changes like diet and sport activity state, are the first line of therapy. In case of ineffective treatment, pharmacological treatments such as laxatives, secretagogues, serotonergic agonists, and many other medications should be induced. If pharmacologic treatment fails, the definitive solution for constipation might be surgical approach. Commonness of this disorder, costs of medical care and decrease in quality life cause constipation is a serious issue for many specialists. The aim of this review is to present current knowledge of chronic constipation and management of this disorder.
Combining Site Characterization, Monitoring and Hydromechanical Modeling for Assessing Slope Stability
Authors: Moradi Shirin , Heinze Thomas , Budler Jasmin , Gunatilake Thanushika , Kemna Andreas , Huisman Johan Alexander
Published: April 2021

Rainfall-induced landslides are a disastrous natural hazard causing loss of life and significant damage to infrastructure, farmland and housing. Hydromechanical models are one way to assess the slope stability and to predict critical combinations of groundwater levels, soil water content and precipitation. However, hydromechanical models for slope stability evaluation require knowledge about mechanical and hydraulic parameters of the soils, lithostratigraphy and morphology. In this work, we present a multi-method approach of site characterization and investigation in combination with a hydromechanical model for a landslide-prone hillslope near Bonn, Germany. The field investigation was used to construct a three-dimensional slope model with major geological units derived from drilling and refraction seismic surveys. Mechanical and hydraulic soil parameters were obtained from previously published values for the study site based on laboratory analysis. Water dynamics were monitored through geoelectrical monitoring, a soil water content sensor network and groundwater stations. Historical data were used for calibration and validation of the hydromechanical model. The well-constrained model was then used to calculate potentially hazardous precipitation events to derive critical thresholds for monitored variables, such as soil water content and precipitation. This work introduces a potential workflow to improve numerical slope stability analysis through multiple data sources from field investigations and outlines the usage of such a system with respect to a site-specific early-warning system.
Assessing Land Use and Land Cover Change and Farmers’ Perceptions of Deforestation and Land Degradation in South-West Côte d’Ivoire, West Africa
Authors: Kouassi Jean-Luc , Gyau Amos , Diby Lucien , Bene Yeboi , Kouamé Christophe
Published: April 2021

Deforestation and land degradation remain two major economic and environmental threats in Côte d’Ivoire. This study assessed land use and land cover (LULC) change and farmers’ perceptions of the drivers and effects of deforestation and land degradation in south-western Côte d’Ivoire. We used remotely sensed data to determine LULC change trends, and a household survey to collect farmers’ perceptions of deforestation and land degradation. A total of 411 households were interviewed using a structured questionnaire and the focus group discussions involved 25 farmers. Landsat image analysis reported a drastic LULC change and a conversion of forestlands into agriculture from 1987 to 2015 at a rate of 1.44%/year and 3.44%/year for dense forests and degraded forests, respectively. The household survey revealed that the major causes of deforestation perceived by farmers included population growth (79.3%), extensive agriculture (72.9%), migration (54.2%) and logging (47.7%). Land degradation evolved, from a shortened fallow period (46.7%) and an inappropriate application of inputs (31.4%). The perceived major effects linked to deforestation encompassed land degradation (70.6%), loss of biodiversity (63.8%), global warming (56.9%) and loss of livelihood assets (54.3%). Therefore, this study recommends participatory landscape planning, reforestation and capacity building of stakeholders for sustainable intensification of the production systems to reduce LULC challenges for enhanced productive and protective functions of remaining forests.
Symmetries and Invariant Solutions for the Coagulation of Aerosols
Authors: Zheng Mingliang
Published: April 2021

The coagulation of aerosol particles plays an important role in the structural morphological changes of suspended particles at any time and in any space. In this study, based on the Smoluchowski equation of population balance, a kinetic model of aerosol coalescence considering Brownian motion collision is established. By applying the developed Lie group method, we derive the allowed infinitesimal symmetries and group-invariant solutions of the integro-differential equation, as well as the exact solution under some special conditions. We also provide detailed steps and a discussion of the properties. The content and results provide an effective analytic solution for the progressive evolution of aerosol particle size considering boundary and initial conditions. This solution reveals the self-conservative phenomena in the process of aerosol coalescence and also provides validation for the numerical algorithms of general dynamics equations.
Hybrid Nanofluid Flow over a Permeable Shrinking Sheet Embedded in a Porous Medium with Radiation and Slip Impacts
Authors: Abu Bakar Shahirah , Md Arifin Norihan , Khashi’ie Najiyah Safwa , Bachok Norfifah
Published: April 2021

The study of hybrid nanofluid and its thermophysical properties is emerging since the early of 2000s and the purpose of this paper is to investigate the flow of hybrid nanofluid over a permeable Darcy porous medium with slip, radiation and shrinking sheet. Here, the hybrid nanofluid consists of Cu/water as the base nanofluid and Al2O3–Cu/water works as the two distinct fluids. The governing ordinary differential equations (ODEs) obtained in this study are converted from a series of partial differential equations (PDEs) by the appropriate use of similarity transformation. Two methods of shooting and bvp4c function are applied to solve the involving physical parameters over the hybrid nanofluid flow. From this study, we conclude that the non-uniqueness of solutions exists through a range of the shrinking parameter, which produces the problem of finding a bigger solution than any other between the upper and lower branches. From the analysis, one can observe the increment of heat transfer rate in hybrid nanofluid versus the traditional nanofluid. The results obtained by the stability of solutions prove that the upper solution (first branch) is stable and the lower solution (second branch) is not stable.
Rapid Hemostatic Biomaterial from a Natural Bath Sponge Skeleton
Authors: Wang Qinghua , Chen Jingwei , Wang Dexiang , Shen Minghui , Ou Huilong , Zhao Jing , Chen Ming , Yan Guoliang , Chen Jun
Published: April 2021

Uncontrolled bleeding is the main cause of mortality from trauma. Collagen has been developed as an important hemostatic material due to its platelet affinity function. A bath sponge skeleton is rich in collagen, also known as spongin. To understand the hemostatic effect of spongin, spongin materials, SX, SFM and SR were prepared from the bath sponge Spongia officinalis, and hemostatic experiments were performed. The SX, SFM and SR were significantly better than the positive control, type I collagen, in shortening the whole blood clotting time in vitro and hemostasis upon rat tail amputation. In a hemostatic experiment of rabbit common carotid artery injury, the hemostatic time and 3 h survival rate of the SFM group were 3.00 ± 1.53 min and 100%, respectively, which are significantly better than those of the commercial hemostat CELOX-A (10.33 ± 1.37 min and 67%, respectively). Additionally, the SFM showed good coagulation effects in platelet-deficient blood and defibrinated blood, while also showing good biocompatibility. Through a variety of tests, we speculated that the hemostatic activity of the SFM is mainly caused by its hyperabsorbency, high affinity to platelets and high effective concentration. Overall, the SFM and spongin derivates could be potential hemostatic agents for uncontrolled bleeding and hemorrhagic diseases caused by deficiency or dysfunction of coagulation factors.
Enhancement of Xanthophyll Synthesis in Porphyra/Pyropia Species (Rhodophyta, Bangiales) by Controlled Abiotic Factors: A Systematic Review and Meta-Analysis
Authors: Piña Florentina , Contreras-Porcia Loretto
Published: April 2021

Red alga species belonging to the Porphyra and Pyropia genera (commonly known as Nori), which are widely consumed and commercialized due to their high nutritional value. These species have a carotenoid profile dominated by xanthophylls, mostly lutein and zeaxanthin, which have relevant benefits for human health. The effects of different abiotic factors on xanthophyll synthesis in these species have been scarcely studied, despite their health benefits. The objectives of this study were (i) to identify the abiotic factors that enhance the synthesis of xanthophylls in Porphyra/Pyropia species by conducting a systematic review and meta-analysis of the xanthophyll content found in the literature, and (ii) to recommend a culture method that would allow a significant accumulation of these compounds in the biomass of these species. The results show that salinity significantly affected the content of total carotenoids and led to higher values under hypersaline conditions (70,247.91 µg/g dm at 55 psu). For lutein and zeaxanthin, the wavelength treatment caused significant differences between the basal and maximum content (4.16–23.47 µg/g dm). Additionally, in Pyropia spp., the total carotenoids were considerably higher than in Porphyra spp.; however, the lutein and zeaxanthin contents were lower. We discuss the specific conditions for each treatment and the relation to the ecological distribution of these species.
Antifungal and Larvicidal Activities of Phlorotannins from Brown Seaweeds
Authors: Negara Bertoka Fajar Surya Perwira , Sohn Jae-Hak , Kim Jin-Soo , Choi Jae-Suk
Published: April 2021

Phlorotannins are secondary metabolites produced by brown seaweeds with antiviral, antibacterial, antifungal, and larvicidal activities. Phlorotannins’ structures are formed by dibenzodioxin, ether and phenyl, ether, or phenyl linkages. The polymerization of phlorotannins is used to classify and characterize. The structural diversity of phlorotannins grows as polymerization increases. They have been characterized extensively with respect to chemical properties and functionality. However, review papers of the biological activities of phlorotannins have focused on their antibacterial and antiviral effects, and reviews of their broad antifungal and larvicidal effects are lacking. Accordingly, evidence for the effectiveness of phlorotannins as antifungal and larvicidal agents is discussed in this review. Online databases (ScienceDirect, PubMed, MEDLINE, and Web of Science) were used to identify relevant articles. In total, 11 articles were retrieved after duplicates were removed and exclusion criteria were applied. Phlorotannins from brown seaweeds show antifungal activity against dermal and plant fungi, and larvicidal activity against mosquitos and marine invertebrate larvae. However, further studies of the biological activity of phlorotannins against fungal and parasitic infections in aquaculture fish, livestock, and companion animals are needed for systematic analyses of their effectiveness. The research described in this review emphasizes the potential applications of phlorotannins as pharmaceutical, functional food, pesticide, and antifouling agents.
Microstructure and Thermomechanical Characterization of Fe-28Mn-6Si-5Cr Shape Memory Alloy
Authors: Collazo Antonio , Figueroa Raúl , Mariño-Martínez Carmen , Pérez Carmen
Published: April 2021

Iron-based shape memory alloys (SMAs) have been widely studied during the last years, producing new formulations with potential applications in civil engineering. In the present paper, the microstructure and the thermomechanical behavior of the Fe-28Mn-6Si-5Cr memory alloy has been investigated. At room temperature, the presence of ε-martensite and γ-austenite was confirmed using optical and electron microscopy techniques. The martensitic transformation temperatures (As, Af, Ms, and Mf) were determined by differential scanning calorimetry, together with an X-ray diffraction technique. The use of these techniques also confirmed that this transformation is not totally reversible, depending on the strain degree and the number of thermal cycles. From the kinetics study of the ε → γ transformation, the isoconversion curves (transformation degree versus time) were built, which provided the information required to optimize the thermal activation cycle. Tensile tests were performed to characterize the mechanical properties of the studied alloy. These kinds of tests were also performed to assess the shape memory effect, getting a recovery stress of 140 MPa, after a 7.6% pre-strain and a thermal activation up to 160 °C.
Urinary Metabolites Enable Differential Diagnosis and Therapeutic Monitoring of Pediatric Inflammatory Bowel Disease
Authors: Yamamoto Mai , Shanmuganathan Meera , Hart Lara , Pai Nikhil , Britz-McKibbin Philip
Published: April 2021

Rates of pediatric Crohn’s disease (CD) and ulcerative colitis (UC) are increasing globally. Differentiation of these inflammatory bowel disease (IBD) subtypes however can be challenging when relying on invasive endoscopic approaches. We sought to identify urinary metabolic signatures of pediatric IBD at diagnosis, and during induction treatment. Nontargeted metabolite profiling of urine samples from CD (n = 18) and UC (n = 8) in a pediatric retrospective cohort study was performed using multisegment injection-capillary electrophoresis-mass spectrometry. Over 122 urinary metabolites were reliably measured from pediatric IBD patients, and unknown metabolites were identified by tandem mass spectrometry. Dynamic changes in sum-normalized urinary metabolites were also monitored following exclusive enteral nutrition (EEN) or corticosteroid therapy (CS) in repeat urine samples collected over 8 weeks. Higher urinary excretion of indoxyl sulfate, hydroxyindoxyl sulfate, phenylacetylglutamine, and sialic acid were measured in CD as compared to UC patients, but lower threonine, serine, kynurenine, and hypoxanthine (p < 0.05). Excellent discrimination of CD from UC was achieved based on the urinary serine:indoxylsulfate ratio (AUC = 0.972; p = 3.21 × 10−5). Urinary octanoyl glucuronide, pantothenic acid, and pyridoxic acid were also identified as specific dietary biomarkers of EEN in pediatric IBD patients who achieved clinical remission. This work may complement or replace existing strategies in the diagnosis and early management of children with IBD.
Changes in Gut Microbiota Correlates with Response to Treatment with Probiotics in Patients with Atopic Dermatitis. A Post Hoc Analysis of a Clinical Trial
Authors: Climent Eric , Martinez-Blanch Juan Francisco , Llobregat Laura , Ruzafa-Costas Beatriz , Carrión-Gutiérrez Miguel Ángel , Ramírez-Boscá Ana , Prieto-Merino David , Genovés Salvador , Codoñer Francisco M. , Ramón Daniel , Chenoll Empar , Navarro-López Vicente
Published: April 2021

Atopic dermatitis (AD) is a chronic recurrent inflammatory skin disease with a high impact on the comfort of those who are affected and long-term treated with corticosteroids with limited efficacy and a high prevalence of relapses. Because of the limited effectiveness of these treatments, new strategies for recovery from AD lesions are continually being explored. In this article, we describe the gut microbiome changes achieved in a recently published clinical trial with the probiotic formulation Bifidobacterium animalis subsp. lactis CECT 8145, Bifidobacterium longum CECT 7347, and Lacticaseibacillus casei CECT 9104 (formerly Lactobacillus casei CECT 9104), showing a significant improvement in SCORAD (scoring atopic dermatitis) index in children (4–17 years) with AD (Clinicaltrials.gov identifier: NCT02585986). The present gut microbiome post hoc study showed no significant changes in diversity (Shannon and Simpson indexes) after probiotic consumption. In the probiotic group, genera Bacteroides, Ruminococcus, and Bifidobacterium significantly increased their levels while Faecalibacterium decreased, compared to the placebo group. Faecalibacterium showed the highest presence and significant positive correlation with AD severity (SCORAD index), whereas Abyssivirga, Bifidobacterium, and Lactococcus were inversely correlated. The results suggest that the consumption of the probiotic formulation here assayed modulates the gut microbiome with significant changes in genera Bacteroides and Faecalibacterium. In turn, the improvement in SCORAD correlates with a decrease in Faecalibacterium and an increase in Bifidobacterium, among others.
Drug Repurposing: A Review of Old and New Antibiotics for the Treatment of Malaria: Identifying Antibiotics with a Fast Onset of Antiplasmodial Action
Authors: Pessanha de Carvalho Lais , Kreidenweiss Andrea , Held Jana
Published: April 2021

Malaria is one of the most life-threatening infectious diseases and constitutes a major health problem, especially in Africa. Although artemisinin combination therapies remain efficacious to treat malaria, the emergence of resistant parasites emphasizes the urgent need of new alternative chemotherapies. One strategy is the repurposing of existing drugs. Herein, we reviewed the antimalarial effects of marketed antibiotics, and described in detail the fast-acting antibiotics that showed activity in nanomolar concentrations. Antibiotics have been used for prophylaxis and treatment of malaria for many years and are of particular interest because they might exert a different mode of action than current antimalarials, and can be used simultaneously to treat concomitant bacterial infections.
Genome-Wide Transcriptomic Identification and Functional Insight of Lily WRKY Genes Responding to Botrytis Fungal Disease
Authors: Kumari Shipra , Kanth Bashistha Kumar , Ahn Ju young , Kim Jong Hwa , Lee Geung-Joo
Published: April 2021

Genome-wide transcriptome analysis using RNA-Seq of Lilium longiflorum revealed valuable genes responding to biotic stresses. WRKY transcription factors are regulatory proteins playing essential roles in defense processes under environmental stresses, causing considerable losses in flower quality and production. Thirty-eight WRKY genes were identified from the transcriptomic profile from lily genotypes, exhibiting leaf blight caused by Botrytis elliptica. Lily WRKYs have a highly conserved motif, WRKYGQK, with a common variant, WRKYGKK. Phylogeny of LlWRKYs with homologous genes from other representative plant species classified them into three groups- I, II, and III consisting of seven, 22, and nine genes, respectively. Base on functional annotation, 22 LlWRKY genes were associated with biotic stress, nine with abiotic stress, and seven with others. Sixteen unique LlWRKY were studied to investigate responses to stress conditions using gene expression under biotic and abiotic stress treatments. Five genes—LlWRKY3, LlWRKY4, LlWRKY5, LlWRKY10, and LlWRKY12—were substantially upregulated, proving to be biotic stress-responsive genes in vivo and in vitro conditions. Moreover, the expression patterns of LlWRKY genes varied in response to drought, heat, cold, and different developmental stages or tissues. Overall, our study provides structural and molecular insights into LlWRKY genes for use in the genetic engineering in Lilium against Botrytis disease.
Phytochemical Characterization of By-Products of Habanero Pepper Grown in Two Different Types of Soils from Yucatán, Mexico
Authors: Chel-Guerrero Lilian Dolores , Oney-Montalvo Julio Enrique , Rodríguez-Buenfil Ingrid Mayanín
Published: April 2021

By-products of edible plants may contain potentially useful phytochemicals. Herein, we valorized the by-products of Capsicum chinense by phytochemical characterization of its leaves, peduncles and stems. Plants of habanero pepper were grown in a greenhouse, in polyethylene bags with two soils that were named according to the Maya classification as: K’ankab lu’um (red soil) and Box lu’um (black soil). Habanero pepper by-products were dried using an oven, the extracts were obtained by Ultrasound Assisted Extraction, and phytochemical quantification in all the extracts was conducted by Ultra Performance Liquid Chromatography coupled to Diode Array Detector (UPLC-DAD). Differences in the phytochemical content were observed according to the by-product and soil used. Catechin and rutin showed the highest concentrations in the peduncles of plants grown in both soils. The leaves of plants grown in black soil were rich in myricetin, β-carotene, and vitamin E, and the stems showed the highest protocatechuic acid content. While the leaves of plants grown in red soil were rich in myricetin and vitamin C, the stems showed the highest chlorogenic acid content. This novel information regarding the phytochemical composition of the by-products of C. chinense may be relevant in supporting their potential application in food and pharmaceutical industries.
Wave Propagation in a Fractional Viscoelastic Tissue Model: Application to Transluminal Procedures
Authors: Gomez Antonio , Rus Guillermo , Saffari Nader
Published: April 2021

In this article, a wave propagation model is presented as the first step in the development of a new type of transluminal procedure for performing elastography. Elastography is a medical imaging modality for mapping the elastic properties of soft tissue. The wave propagation model is based on a Kelvin Voigt Fractional Derivative (KVFD) viscoelastic wave equation, and is numerically solved using a Finite Difference Time Domain (FDTD) method. Fractional rheological models, such as the KVFD, are particularly well suited to model the viscoelastic response of soft tissue in elastography. The transluminal procedure is based on the transmission and detection of shear waves through the luminal wall. Shear waves travelling through the tissue are perturbed after encountering areas of altered elasticity. These perturbations carry information of medical interest that can be extracted by solving the inverse problem. Scattering from prostate tumours is used as an example application to test the model. In silico results demonstrate that shear waves are satisfactorily transmitted through the luminal wall and that echoes, coming from reflected energy at the edges of an area of altered elasticity, which are feasibly detectable by using the transluminal approach. The model here presented provides a useful tool to establish the feasibility of transluminal procedures based on wave propagation and its interaction with the mechanical properties of the tissue outside the lumen.
A Raspberry Pi-Based Traumatic Brain Injury Detection System for Single-Channel Electroencephalogram
Authors: Dhillon Navjodh , Sutandi Agustinus , Vishwanath Manoj , Lim Miranda , Cao Hung , Si Dong
Published: April 2021

Traumatic Brain Injury (TBI) is a common cause of death and disability. However, existing tools for TBI diagnosis are either subjective or require extensive clinical setup and expertise. The increasing affordability and reduction in the size of relatively high-performance computing systems combined with promising results from TBI related machine learning research make it possible to create compact and portable systems for early detection of TBI. This work describes a Raspberry Pi based portable, real-time data acquisition, and automated processing system that uses machine learning to efficiently identify TBI and automatically score sleep stages from a single-channel Electroencephalogram (EEG) signal. We discuss the design, implementation, and verification of the system that can digitize the EEG signal using an Analog to Digital Converter (ADC) and perform real-time signal classification to detect the presence of mild TBI (mTBI). We utilize Convolutional Neural Networks (CNN) and XGBoost based predictive models to evaluate the performance and demonstrate the versatility of the system to operate with multiple types of predictive models. We achieve a peak classification accuracy of more than 90% with a classification time of less than 1 s across 16–64 s epochs for TBI vs. control conditions. This work can enable the development of systems suitable for field use without requiring specialized medical equipment for early TBI detection applications and TBI research. Further, this work opens avenues to implement connected, real-time TBI related health and wellness monitoring systems.
Vibration-Sensing Electronic Yarns for the Monitoring of Hand Transmitted Vibrations
Authors: Rahemtulla Zahra , Hughes-Riley Theodore , Dias Tilak
Published: April 2021

Overexposure to hand transmitted vibrations (HTVs) from prolonged use of vibrating power tools can result in severe injuries. By monitoring the exposure of a worker to HTVs, overexposure, and injury, can be mitigated. An ideal HTV-monitoring system would measure vibration were it enters the body, which for many power tools will be the palm and fingers, however this is difficult to achieve using conventional transducers as they will affect the comfort of the user and subsequently alter the way that the tool is held. By embedding a transducer within the core of a textile yarn, that can be used to produce a glove, vibration can be monitored close to where it enters the body without compromising the comfort of the user. This work presents a vibration-sensing electronic yarn that was created by embedding a commercially available accelerometer within the structure of a yarn. These yarns were subsequently used to produce a vibration-sensing glove. The purpose of this study is to characterize the response of the embedded accelerometer over a range of relevant frequencies and vibration amplitudes at each stage of the electronic yarn’s manufacture to understand how the yarn structure influences the sensors response. The vibration-sensing electronic yarn was subsequently incorporated into a fabric sample and characterized. Finally, four vibration-sensing electronic yarns were used to produce a vibration-sensing glove that is capable of monitoring vibration at the palm and index finger.
AMBtalk: A Cardiovascular IoT Device for Ambulance Applications
Authors: Chen Wen-Liang , Lin Yi-Bing , Chang Ted C.-Y. , Lin Yan-Ren
Published: April 2021

Acute Coronary Syndrome (ACS) and other heart emergency events require immediate chest pain identification in the ambulance. Specifically, early identification and triage is required so that patients with chest pain can be quickly sent to a hospital with appropriate care facilities for treatment. In the traditional approach, ambulance personnel often use symptom checklists to examine the patient and make a quick decision for the target hospital. However, not every hospital has specialist facilities to handle such emergency cases. If the result of the subsequent cardiac enzyme test performed at the target hospital strongly suggests the occurrence of myocardial infarction, the patient may need to be sent to another hospital with specialist facilities, such as Percutaneous Coronary Intervention. The standard procedure is time consuming, which may result in delayed treatment and reduce patent survival rate. To resolve this issue, we propose AMBtalk (Ambulance Talk) for accurate, early ACS identification in an ambulance. AMBtalk provides real-time connection to hospital resources, which reduces the elapsed time for treatment, and therefore, improves the patient survival rate. The key to success for AMBtalk is the development of the AllCheck® Internet of Things (IoT) device, which can accurately and quickly provide cardiovascular parameter values for early ACS identification. The interactions between the AllCheck® IoT device, the emergency medical service center, the ambulance personnel and the hospital are achieved through the AMBtalk IoT server in the cloud network. AllCheck® outperforms the existing cardiovascular IoT device solutions for ambulance applications. The testing results of the AllCheck® device show 99% correlation with the results of the hospital reports. Due to its excellent performance in quick ACS identification, the AllCheck® device was awarded the 17th Taiwan Innovators Award in 2020.
A New Approach to Power Efficiency Improvement of Ultrasonic Transmitters via a Dynamic Bias Technique
Authors: Kim Kyeongjin , Choi Hojong
Published: April 2021

To obtain a high-quality signal from an ultrasound system through the transmitter, it is necessary to achieve an appropriate operating point of the power amplifier in the ultrasonic transmitter by applying high static bias voltage. However, the power amplifier needs to be operated at low bias voltage, because a power amplifier operating at high bias voltage may consume a large amount of power and increase the temperature of the active devices, worsening the signal characteristics of the ultrasound systems. Therefore, we propose a new method of increasing the bias voltage for a specific period to solve this problem by reducing the output signal distortion of the power amplifier and decreasing the load on the active device. To compare the performance of the proposed method, we measured and compared the signals of the amplifier with the proposed technique and the amplifier only. Notably, improvement was achieved with 11.1% of the power added efficiency and 3.23% of the total harmonic distortion (THD). Additionally, the echo signal generated by the ultrasonic transducer was improved by 2.73 dB of amplitude and 0.028% of THD under the conditions of an input signal of 10 mW. Therefore, the proposed method could be useful for improving ultrasonic transmitter performance using the developed technique.
Hazard Perception–Response: A Theoretical Framework to Explain Drivers’ Interactions with Roadway Hazards
Authors: Barragan Daniela , Peterson Matthew S. , Lee Yi-Ching
Published: April 2021

Research suggests that novice drivers are most susceptible to errors when detecting and responding to hazards. If this were true, then hazard training should be effective in improving novice drivers’ performance. However, there is limited evidence to support this effectiveness. Much of this research has overlooked a fundamental aspect of psychological research: theory. Although four theoretical frameworks were developed to explain this process, none have been validated. We proposed a theoretical framework to more accurately explain drivers’ behavior when interacting with hazardous situations. This framework is novel in that it leverages support from visual attention and driving behavior research. Hazard-related constructs are defined and suitable metrics to evaluate the stages in hazard processing are suggested. Additionally, individual differences which affect hazard-related skills are also discussed. This new theoretical framework may explain why the conflicts in current hazard-related research fail to provide evidence that training such behaviors reduces crash risk. Future research is necessary to empirically test this framework.
Adaptive Sparse Cyclic Coordinate Descent for Sparse Frequency Estimation
Authors: Garcia Guzman Yuneisy E. , Lunglmayr Michael
Published: April 2021

The frequency estimation of multiple complex sinusoids in the presence of noise is important for many signal processing applications. As already discussed in the literature, this problem can be reformulated as a sparse representation problem. In this letter, such a formulation is derived and an algorithm based on sparse cyclic coordinate descent (SCCD) for estimating the frequency parameters is proposed. The algorithm adaptively reduces the size of the used frequency grid, which eases the computational burden. Simulation results revealed that the proposed algorithm achieves similar performance to the original formulation and the Root-multiple signal classification (MUSIC) algorithm in terms of the mean square error (MSE), with significantly less complexity.
Preclinical Marmoset Model for Targeting Chronic Inflammation as a Strategy to Prevent Alzheimer’s Disease
Authors: Philippens Ingrid H. C. H. M. , Langermans Jan A. M.
Published: April 2021

Due to the aging population, modern society is facing an increasing prevalence of neurological diseases such as Alzheimer’s disease (AD). AD is an age-related chronic neurodegenerative disorder for which no satisfying therapy exists. Understanding the mechanisms underlying the onset of AD is necessary to find targets for protective treatment. There is growing awareness of the essential role of the immune system in the early AD pathology. Amyloidopathy, the main feature of early-stage AD, has a deregulating effect on the immune function. This is reciprocal as the immune system also affects amyloidopathy. It seems that the inflammatory reaction shows a heterogeneous pattern depending on the stage of the disease and the variation between individuals, making not only the target but also the timing of treatment important. The lack of relevant translational animal models that faithfully reproduce clinical and pathogenic features of AD is a major cause of the delay in developing new disease-modifying therapies and their optimal timing of administration. This review describes the communication between amyloidopathy and inflammation and the possibility of using nonhuman primates as a relevant animal model for preclinical AD research.
Attitude of children with autism spectrum disorder to the dental treatment and assessment of their parents’ knowledge about the oral disease prevention (survey data)
Authors: Kiselnikova L. P. , Lyamtseva M. P. , Kuznetsova J. E.
Published: April 2021

Relevance. Autism spectrum disorder (ASD) is a developmental disorder, showing up in children under 3 years old and characterized by the triad of symptoms (qualitative impairment of social interaction and communication, stereotype behavior and non-specific problems) and the dyad of impairment in social interaction, restricted and repetitive patterns of behavior and/ or interests. According to the Russian and international literature, the prevalence of ASD has tended to grow in the 21th century. Children with ASD experience difficulties in communication and manual skills. The oral hygiene in children with ASD is poor, they need their parents’ help brushing their teeth as well as preparing for a dental visit. Thus, improvement of communication techniques is an urgent challenge for these children.Materials and methods. 75 parents of children with ASD aged 4 to 16 years were surveyed.Results. The received results demonstrated that the parents of children with ASD have very little knowledge about the oral hygiene. While preparing for a dental visit the majority of parents do not use communication techniques, which leads to doctor-patient interaction difficulties.Conclusions. The received data showed that techniques for applied behavior analysis, alternative communication system prove to be highly effective in preparing children with ASD for a dental visit, thus, increasing the effectiveness of preventive procedures.
Probability assessment of facial nerve injury in children and adolescents during the elective maxillofacial surgery
Authors: Topolnitsky O. Z. , Askerov E. D.
Published: April 2021

Relevance. Various factors can cause facial neuropathy. Iatrogenic facial nerve injury in childhood and adolescence is a complicated medical, psychological and legal problem. Risk assessment of the facial nerve injury during the elective maxillofacial surgeries is required depending on the localization of the procedure. The statistical assessment is very important for the evaluation of the possible iatrogenic facial neuropathy in children and adolescents during the elective maxillofacial surgeries.Materials and methods. 715 medical records for 2017 from the Department of Pediatric Maxillofacial Surgery of the MSUMD Clinical Center for Maxillofacial, Reconstructive and Plastic Surgery were analyzed.Results. There was a risk of injury to the trunk or branches of the facial nerve during surgery in 121 cases (16,9%) for the technical complexity of the surgical approach and the pathology location.Conclusions. There is a high risk of the facial nerve injury during the elective maxillofacial surgery in children and adolescents due to the complex anatomy of the area. Intraoperative neuromonitoring is recommended to prevent iatrogenic neuropathy of the facial nerve.
Modified PDL anesthetic injection technique in the pediatric dental practice
Authors: Anisimova E. N. , Anisimova N. Yu. , Kovylina O. S. , Eliseev D. A. , Metelitsa Yu. N. , Kravchenko I. A.
Published: April 2021

Relevance. Effective pain relief is required for high-quality outpatient dental treatment in children. Infiltration and PDL local anesthetic injection techniques are easier and safer for young patients. Purpose – to determine the effectiveness and safety of the modified PDL anesthetic injection technique in the pediatric dental practice. Materials and methods. The study involved 100 children aged 4 to 7 years, in need of caries and pulpitis treatment in the primary dentition. 4% articaine solutions with 1: 200000 and 1: 400000 epinephrine were administered as a local anesthetic. The effectiveness of the treatment was determined by the visual analogue scale.Results. The study determined the high effectiveness and safety of the modified technique of PDL anesthetic injection of 4% articaine solution with epinephrine ratio 1:200 000 and 1:400 000 for the treatment of caries and pulpitis in 100 children aged 4-7 years.Conclusions. The conducted studies have demonstrated the high ffectiveness and safety of the modified PDL anesthetic injection technique of a 4% solution of articaine with epinephrine both at a concentration of 1:200 000 and 1:400 000.