bluuebunny/crossref_metadata_2025 huggingface dataset:
Left bundle branch pacing and cardiac remodeling in HF patients with type 2 diabetes mellitus: epigenetic pathways and clinical outcomes
Authors: Sardu Celestino , Vittoria Marfella Ludovica , Giordano Valerio , Lepre Caterina Claudia , D’Amico Giovanbattista , Volpicelli Mario , Contaldi Carla , Galiero Raffaele , Caturano Alfredo , Casolaro Flavia , Sasso Ferdinando Carlo , Uran Carlo , Cozzolino Domenico , Nicoletti Maddalena , Signoriello Giuseppe , Paolisso Giuseppe , Marfella Raffaele
Published: May 2024

Background

Left bundle branch (LBB) pacing could achieve cardiac resynchronization therapy (CRT) in patients who cannot be resynchronized via the placement of the left ventricle (LV) lead into the coronary sinus. LBB pacing could improve cardiovascular outcomes in heart failure (HF) patients with LBB block who are affected by type 2 diabetes mellitus (T2DM).

Study hypothesis

LBB pacing could increase the number of CRT responders and lead to the best clinical outcomes in HF patients with T2DM, inducing cardiac remodeling and improving left ventricle ejection fraction (LVEF) via microRNA (miR) modulation.

Methods

In a multicenter observational study, we enrolled 334 HF patients with LBB block and an indication to receive LBB pacing for CRT. In these patients, we evaluated the CRT responder rate, clinical outcomes, and miR expression at 1 year of follow-up.

Results

At 1 year of follow-up, we had 223 responders (66.8%), 132 hospitalizations for HF (39.5%), 24 cardiac deaths (7.2%), and 37 all-cause deaths (11.1%), with a higher rate of HF hospitalizations (77 (69.4%) vs 55 (24.7%), p < 0.05), and cardiac deaths (13 (11.7% vs 11 (4.9%), p < 0.05) in non-responders vs responders. At the end of follow-up, we found the lowest expression of miR-26, miR-29, miR-30, miR-92, and miR-145 in LBB-pacing non-responders vs responders (p < 0.05), and a direct correlation between miR-30 (0.340, [0.833–1.915]; p 0.001), the 6-minute-walking test (6MWT; 0.168, [0.008–0.060]; p 0.011), angiotensin-receptor-neprilysin inhibitors (ARNI; 0.157, [0.183–4.877]; p 0.035), sodium-glucose-transporter-2 inhibitors (0.245, [2.242–7.283]; p 0.001), and LVEF improvements. C reactive protein (CRP) inversely correlated with LVEF improvement (−0.220, [-(0.066–0.263)]; p 0.001). ARNI (1.373, CI 95% [1.007–1.872], p 0.045), miR-30 (2.713, CI 95% [1.543–4.769], p 0.001), and 6MWT (1.288, CI 95% [1.084–1.998], p 0.001) were predictors of LBB pacing responders at 1 year of follow-up.

Conclusion

LBB-pacing responders evidenced miR modulation, which was linked to significant improvement of the cardiac pump. Specifically, miR-30 was linked to cardiac pump improvement and predicted responders at 1 year of follow-up in patients with T2DM.
Exploration of the mechanism of Traditional Chinese Medicine for anxiety and depression in patients with diarrheal irritable bowel syndrome based on network pharmacology and meta-analysis
Authors: Bai Chen , Wang Junyi , Wang Yifan , Liu Haoqi , Li Jiaxiu , Wang Siyi , Bai Zhen , Guo Rongjuan
Published: May 2024

Background

The efficacy of Chinese herbal medicine (CHM) in managing irritable bowel syndrome with diarrhea (IBS-D) accompanied by anxiety and depression remains uncertain. Thus, a systematic review was carried out employing meta-analysis and network pharmacology to ascertain the efficacy and underlying mechanisms of CHM therapy.

Methods

By conducting a systematic review, including literature search, screening, and data extraction, we identified 25 randomized controlled trials to assess CHM’s effectiveness in treating irritable bowel syndrome alongside anxiety and depression. Network pharmacology was utilized to scrutinize the metabolite utility of CHM in addressing this condition. Potential primary mechanisms were synthesized using information sourced from the PubMed database.

Results

Twenty-five studies, including 2055 patients, were analyzed, revealing significant treatment efficacy for IBS-D in the trial group compared to controls [OR = 4.01, 95% CI (2.99, 5.36), I2 = 0%] Additionally, treatment for depression [SMD = −1.08, 95% CI (-1.30, −0.86), p < 0.00001, I2 = 68%; SDS: SMD = -1.69, 95% CI (-2.48, −0.90), p < 0.0001, I2 = 96%] and anxiety [HAMA: SMD = -1.29, 95% CI (-1.68, −0.91), p < 0.00001, I2 = 89%; SAS: SMD = -1.75, 95% CI (-2.55, −0.95), p < 0.00001, I2 = 96%] significantly improved in the trial group. Furthermore, the trial group exhibited a significantly lower disease relapse rate [OR = 0.30, 95% CI (0.20, 0.44), p < 0.00001, I2 = 0%]. CHM treatment consistently improved IBS severity (IBS-SSS) and symptom scores. Network pharmacology analysis identified key chemical metabolites in traditional Chinese medicine formulations, including Beta-sitosterol, Stigmasterol, Quercetin, Naringenin, Luteolin, Kaempferol, Nobiletin, Wogonin, Formononetin, and Isorhamnetin. Utilizing the STRING database and Cytoscape v3.9.0 software, a protein-protein interaction (PPI) network revealed the top eight key targets: IL-6, TNF, PPARG, PTGS2, ESR1, NOS3, MAPK8, and AKT1, implicated in anti-inflammatory responses, antioxidant stress modulation, and neurotransmitter homeostasis maintenance.

Conclusion

Chinese Herbal Medicine (CHM) offers a promising and safe treatment approach for patients dealing with Diarrheal Irritable Bowel Syndrome (IBS-D) accompanied by anxiety and depression; thus, indicating its potential for practical implementation. The most active metabolites of CHM could simultaneously act on the pathological targets of IBS-D, anxiety, and depression.The diverse scope of CHM’s therapeutic role includes various aspects and objectives, underscoring its potential for broad utilization.
GWAS analysis reveals the genetic basis of blast resistance associated with heading date in rice
Authors: Lee Seung Young , Lee Gileung , Han Jiheon , Ha Su-Kyung , Lee Chang-Min , Kang Kyeongmin , Jin Mina , Suh Jung-Pil , Jeung Ji-Ung , Mo Youngjun , Lee Hyun-Sook
Published: May 2024

Rice blast is a destructive fungal disease affecting rice plants at various growth stages, significantly threatening global yield stability. Development of resistant rice cultivars stands as a practical means of disease control. Generally, association mapping with a diversity panel powerfully identifies new alleles controlling trait of interest. On the other hand, utilization of a breeding panel has its advantage that can be directly applied in a breeding program. In this study, we conducted a genome-wide association study (GWAS) for blast resistance using 296 commercial rice cultivars with low population structure but large phenotypic diversity. We attempt to answer the genetic basis behind rice blast resistance among early maturing cultivars by subdividing the population based on its Heading date 1 (Hd1) functionality. Subpopulation-specific GWAS using the mixed linear model (MLM) based on blast nursery screening conducted in three years revealed a total of 26 significant signals, including three nucleotide-binding site leucine-rich repeat (NBS-LRR) genes (Os06g0286500, Os06g0286700, and Os06g0287500) located at Piz locus on chromosome 6, and one at the Pi-ta locus (Os12g0281300) on chromosome 12. Haplotype analysis revealed blast resistance associated with Piz locus was exclusively specific to Type 14 hd1 among japonica rice. Our findings provide valuable insights for breeding blast resistant rice and highlight the applicability of our elite cultivar panel to detect superior alleles associated with important agronomic traits.
A systematic review of personality and musculoskeletal disorders: evidence from general population studies
Authors: Quirk Shae E. , Koivumaa-Honkanen Heli , Honkanen Risto J. , Mohebbi Mohammadreza , Stuart Amanda L. , Heikkinen Jeremi , Williams Lana J.
Published: May 2024

Introduction

We conducted a systematic review to evaluate the quality and extent of evidence on associations between personality disorders (PDs) and musculoskeletal disorders (MSDs) in population-based studies, since these disorders are leading causes of disease burden worldwide.

Methods

A search strategy of published, peer-reviewed and gray literature was developed in consultation with a liaison librarian and implemented for Embase, CINAHL Complete, Medline Complete, and PsycINFO via the EBSCOhost platform from 1990 to the present and CORDIS and ProQuest Dissertations & Theses Global, respectively. The inclusion criteria were as follows: I) general population participants aged ≥15 years; II) self-report, probable PD based on positive screen, or threshold PD according to the DSM-IV/5 (groupings: any, Clusters A/B/C, specific PD) or ICD-10/11; III) MSDs identified by self-report or ICD criteria (arthritis, back/neck conditions, fibromyalgia, osteopenia/osteoporosis) and III) cohort, case-control, and cross-sectional study designs. Two reviewers independently screened articles and extracted the data. Critical appraisal was undertaken using the Joanna Briggs Institute checklists for systematic reviews of etiology and risk. A descriptive synthesis presents the characteristics of included studies, critical appraisal results, and descriptions of the main findings. This review adhered to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines.

Results

There were 11 peer-reviewed, published articles included in this review (n = 9 cross-sectional and n = 2 case-control studies); participants were ≥18 years in these studies. No published gray literature was identified. Semi-structured interviews were the most common method to ascertain PDs; all studies utilized self-reported measures to identify MSDs. Overall, we detected limited and conflicting evidence for associations between PDs and MSDs.

Discussion

The main result may be explained by lack of population-based longitudinal evidence, heterogenous groupings of PD, and few comparable cross-sectional and case-control studies. Strengths of the review include a comprehensive search strategy and a discussion of mechanisms underlying possible associations between PDs and MSDs.

Conclusions

The quality of most studies included in this review that examined associations between PD and MSDs in general population adults was high. However, the results demonstrated limited and conflicting evidence for these associations, in part, due to lack of comparable evidence, which should be addressed in future research.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42021243094.
Communal nesting shapes the sex-dependent glutamatergic response to early life stress in the rat prefrontal cortex
Authors: Mottarlini Francesca , Rizzi Beatrice , Targa Giorgia , Buzzelli Valeria , Di Trapano Melania , Rullo Laura , Candeletti Sanzio , Ciccocioppo Roberto , Fattore Liana , Romualdi Patrizia , Fumagalli Fabio , Trezza Viviana , Caffino Lucia
Published: May 2024

Introduction

Early social environment, either positive or negative, shapes the adult brain. Communal nesting (CN), a naturalistic setting in which 2-3 females keep their pups in a single nest sharing care-giving behavior, provides high level of peer interaction for pups. Early social isolation (ESI) from dam and siblings represents, instead, an adverse condition providing no peer interaction.

Methods

We investigated whether CN (enrichment setting) might influence the response to ESI (impoverishment setting) in terms of social behavior and glutamate system in the medial prefrontal cortex (mPFC) of adult and adolescent male and female rats.

Results

Pinning (a rewarding component of social play behavior) was significantly more pronounced in males than in females exposed to the combination of CN and ESI. CN sensitized the glutamate synapse in the mPFC of ESI-exposed male, but not female, rats. Accordingly, we observed (i) a potentiation of the glutamatergic neurotransmission in the mPFC of both adolescent and adult males, as shown by the recruitment of NMDA receptor subunits together with increased expression/activation of PSD95, SynCAM 1, Synapsin I and αCaMKII; (ii) a de-recruiting of NMDA receptors from active synaptic zones of same-age females, together with reduced expression/activation of the above-mentioned proteins, which might reduce the glutamate transmission. Whether similar sex-dependent glutamate homeostasis modulation occurs in other brain areas remains to be elucidated.

Discussion

CN and ESI interact to shape social behavior and mPFC glutamate synapse homeostasis in an age- and sex-dependent fashion, suggesting that early-life social environment may play a crucial role in regulating the risk to develop psychopathology.
Long-term gastrointestinal symptoms and sleep quality sequelae in adolescents after COVID-19: a retrospective study
Authors: Yang Wei-Lin , Wang Qi , Wang Ying , Sun Shaopeng , Shen Yan , Yu Lei-Min
Published: May 2024

Objective

To evaluate the long-term gastrointestinal (GI) symptoms and sleep quality sequelae in adolescents with COVID-19.

Methods

Between June and July 2023, an online survey was done in Xiaoshan District, Hangzhou City, Zhejiang Province, China, using the GI Symptom Rating Scale (GSRS) and the Pittsburgh Sleep Quality Inventory (PSQI).

Results

GI symptoms in COVID-19 patients increased by 11.86% compared to before infection, while sleep quality decreased by 10.9%. Over time, there was a significant increase in the cumulative incidence rate of GI symptoms and sleep disorders (p < 0.001). Follow-up of COVID-19 positive patients within 6 months of infection showed that GI symptoms and sleep quality began to ease starting from the first month after infection. Further analysis indicated a significant linear relationship between the severity of GI symptoms and sleep quality (R > 0.5, p < 0.001). Moreover, females, older age, and higher education were identified as risk factors influencing the long-term effects of COVID-19.

Conclusion

SARS-CoV-2 affects GI symptoms and sleep quality in adolescents during both the acute phase and post-infection periods. Over time, these symptoms gradually alleviate. A significant correlation exists between GI symptoms and sleep quality.
Blood cadmium level as a risk factor for chronic pain: NHANES database 1999–2004
Authors: Mi Panpan , Dong Haoran , Chen Shengle , Gao Xuan , Cao Xu , Liu Yong , Wang Huijie , Fan Guofeng
Published: May 2024

Objective

The escalating prevalence of chronic pain poses a substantial socio-economic burden. Chronic pain primarily stems from musculoskeletal and nervous system impairments. Given cadmium's known toxicity to these systems, our study sought to investigate the correlation between blood cadmium levels and chronic pain.

Methods

The cross-sectional study was conducted from the National Health and Nutrition Examination Survey (NHANES, 1999–2004), and comprised US adults who participated in a chronic pain interview. We employed logistic regression models and smooth curve fitting to elucidate the relationship between blood cadmium levels and chronic pain.

Results

Our findings revealed a linear association between blood cadmium levels and chronic pain. Compared to the lower blood cadmium tertile 1 (<0.3 ug/dL), the adjusted odds ratios (ORs) for tertile 2 (0.3–0.4 ug/dL), and tertile 3 (≥0.5 ug/dL), were 1.11 (0.96–1.29) and 1.2 (1.03–1.39), respectively. Sensitivity analyses corroborated these results.

Conclusion

Elevated levels of blood cadmium are associated with a heightened risk of chronic pain among adults in the United States. Mitigating cadmium exposure could potentially decrease the risk of chronic pain, thereby enhancing strategies for chronic pain prevention and management.
COVID-19 outbreaks in residential aged care facilities: an agent-based modeling study
Authors: McAndrew Fenella , Sacks-Davis Rachel , Abeysuriya Romesh G. , Delport Dominic , West Daniel , Parta Indra , Majumdar Suman , Hellard Margaret , Scott Nick
Published: May 2024

Introduction

A disproportionate number of COVID-19 deaths occur in Residential Aged Care Facilities (RACFs), where better evidence is needed to target COVID-19 interventions to prevent mortality. This study used an agent-based model to assess the role of community prevalence, vaccination strategies, and non-pharmaceutical interventions (NPIs) on COVID-19 outcomes in RACFs in Victoria, Australia.

Methods

The model simulated outbreaks in RACFs over time, and was calibrated to distributions for outbreak size, outbreak duration, and case fatality rate in Victorian RACFs over 2022. The number of incursions to RACFs per day were estimated to fit total deaths and diagnoses over time and community prevalence.

Total infections, diagnoses, and deaths in RACFs were estimated over July 2023–June 2024 under scenarios of different: community epidemic wave assumptions (magnitude and frequency); RACF vaccination strategies (6-monthly, 12-monthly, no further vaccines); additional non-pharmaceutical interventions (10, 25, 50% efficacy); and reduction in incursions (30% or 60%).

Results

Total RACF outcomes were proportional to cumulative community infections and incursion rates, suggesting potential for strategic visitation/staff policies or community-based interventions to reduce deaths. Recency of vaccination when epidemic waves occurred was critical; compared with 6-monthly boosters, 12-monthly boosters had approximately 1.2 times more deaths and no further boosters had approximately 1.6 times more deaths over July 2023–June 2024. Additional NPIs, even with only 10–25% efficacy, could lead to a 13–31% reduction in deaths in RACFs.

Conclusion

Future community epidemic wave patterns are unknown but will be major drivers of outcomes in RACFs. Maintaining high coverage of recent vaccination, minimizing incursions, and increasing NPIs can have a major impact on cumulative infections and deaths.
Fine particulate matter-sudden death association modified by ventricular hypertrophy and inflammation: a case-crossover study
Authors: Rappazzo Kristen M. , Egerstrom Nicole M. , Wu Jianyong , Capone Alia B. , Joodi Golsa , Keen Susan , Cascio Wayne E. , Simpson Ross J.
Published: May 2024

Background

Sudden death accounts for approximately 10% of deaths among working-age adults and is associated with poor air quality. Objectives: To identify high-risk groups and potential modifiers and mediators of risk, we explored previously established associations between fine particulate matter (PM2.5) and sudden death stratified by potential risk factors.

Methods

Sudden death victims in Wake County, NC, from 1 March 2013 to 28 February 2015 were identified by screening Emergency Medical Systems reports and adjudicated (n = 399). Daily PM2.5 concentrations for Wake County from the Air Quality Data Mart were linked to event and control periods. Potential modifiers included greenspace metrics, clinical conditions, left ventricular hypertrophy (LVH), and neutrophil-to-lymphocyte ratio (NLR). Using a case-crossover design, conditional logistic regression estimated the OR (95%CI) for sudden death for a 5 μg/m3 increase in PM2.5 with a 1-day lag, adjusted for temperature and humidity, across risk factor strata.

Results

Individuals having LVH or an NLR above 2.5 had PM2.5 associations of greater magnitude than those without [with LVH OR: 1.90 (1.04, 3.50); NLR > 2.5: 1.25 (0.89, 1.76)]. PM2.5 was generally less impactful for individuals living in areas with higher levels of greenspace.

Conclusion

LVH and inflammation may be the final step in the causal pathway whereby poor air quality and traditional risk factors trigger arrhythmia or myocardial ischemia and sudden death. The combination of statistical evidence with clinical knowledge can inform medical providers of underlying risks for their patients generally, while our findings here may help guide interventions to mitigate the incidence of sudden death.
Efficacy of telemedicine intervention in the self-management of patients with type 2 diabetes: a systematic review and meta-analysis
Authors: Liu Fengzhao , Li Jixin , Li Xiangyu , Yang Zhenyu , Wang Wenru , Zhao Lijuan , Wu Tao , Huang Chengcheng , Xu Yunsheng
Published: May 2024

Purpose

We aimed to report the latest and largest pooled analyses and evidence updates to assess the effectiveness of telemedicine interventions for self-management (DSM) in patients with type 2 diabetes mellitus (T2DM).

Methods

A systematic literature search was conducted using PubMed, Cochrane, Embase, and Web of Science in December 2023. We included randomized controlled trials (RCTs) of adults (≥18 years of age) diagnosed with T2DM where the intervention was the application of telemedicine. The Cochrane Risk of Bias Assessment was used to evaluate quality. The study’s main outcome indicators were glycosylated hemoglobin (HbA1c) and diabetes self-management (DSM) capacity.

Results

A total of 17 eligible articles, comprising 20 studies and 1,456 patients (734 in the intervention group and 722 in the control group), were included in the evidence synthesis. The baseline characteristics of both groups were similar in all outcomes. Comprehensive analyses showed post-intervention decreases in HbA1c, 2-h postprandial glucose, systolic and diastolic blood pressure, increases in Diabetes Self- Care activities, DSM competencies based on dietary and medication adherence, and improvements in overall DSM scores, all of which were statistically significant. While no statistically significant differences were observed in body mass index, lipids, and other DSM dimensions. Based on subgroup analyses, app-based experimental interventions targeting under 60 years old populations in Asia and North America were found to be more effective and less heterogeneity in the short term (<6 months of intervention).

Conclusion

Telemedicine interventions may assist patients with T2DM in enhancing their DSM and improving their HbA1c levels. Clinician can use various telemedicine interventions to enhance DSM in T2DM patients, considering local circumstances.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, CRD42024508522.
Varroa destructor economic injury levels and pathogens associated with colony losses in Western Canada
Authors: Morfin Nuria , Foster Leonard J. , Guzman-Novoa Ernesto , Van Westendorp Paul , Currie Robert W. , Higo Heather
Published: May 2024

Introduction

The honey bee parasitic mite, Varroa destructor, is considered the main factor associated with winter colony losses. To control this mite, beekeepers must implement Integrated Pest Management (IPM) strategies. An effective IPM strategy is based on timely monitoring of mite levels to treat the colonies when the mites reach an economic threshold and before they grow to damaging levels (economic injury level). Additionally, concerns have been raised about the possible interaction between V. destructor and other stressors; stressors acting additively or synergistically may lead to high colony mortality.

Methodology

Varroa destructor levels were recorded in colonies from five regions of British Columbia, Canada: Lower Mainland, Kootenay, Okanagan, Cariboo, and Peace. Mite levels were analyzed for associations with colony strength and mortality, as well as with other pathogens that were identified using total RNA sequencing.

Results

The Kootenay and Lower Mainland regions had the highest V. destructor levels in the fall (3.38±0.65 and 2.51±0.57, respectively) followed by the Okanagan (1.54±0.36), Cariboo (1.31±0.39), and Peace (0.66±0.25) regions. The Peace region had a significantly lower proportion of dead colonies than the other regions. Varroa destructor levels in the fall significantly predicted odds of colony mortality in the spring. Colonies with ≥ 1% mite infestation in the fall (August) had a significantly higher mortality rate (p=0.005) compared to colonies with <1% mite levels. Therefore, treatment is recommended above that threshold. Also, deformed wing virus-B levels were 8.4 times higher than those of deformed wing virus-A variant in all the regions and were highest in the Kootenay region, which also had the highest rate of colony losses. Lastly, Malpighamoeba mellificae and Nosema (Vairimorpha) apis transcripts were identified along with other pathogens that deserve further investigation, including Apis mellifera filamentous virus, Apis rhabdovirus, Lake Sinai virus, Varroa tymo-like, and Apis mellifera solinvivirus 1.

Discussion

The dynamic nature of host-pathogen interactions requires frequent pathogen surveillance to determine the prevalence and levels of parasitic mites and associated infectious diseases, and their impact on honey bee health, not only in the regions of this study, but globally. Nevertheless, more studies are needed to determine the nature of the type of interaction between the identified pathogens and V. destructor. Also, more research is needed to evaluate their potential impact on colony survival, to provide accurate mite and disease control recommendations to beekeepers.
Long-term follow-up results of prostate capsule-sparing and nerve-sparing radical cystectomy with neobladder: a single-center retrospective analysis
Authors: Zhu Zaisheng , Zhu Yiyi , Shi Hongqi , Zhou Penfei , Xue Yadong , Hu Shengye
Published: May 2024

Objective

This study aims to investigate and analyze the feasibility, oncological outcomes, functional efficacy, and complications with the prostatic capsule sparing (PCS) as well as the nerve sparing (NS) in radical cystectomy for bladder cancer.

Patients and methods

Between January 2007 and December 2021, 67 total cystectomies with PCS and 54 with NS were performed at our institution. The inclusion criteria for PCS were as follows: proactive, fully informed patient consent; negative transurethral resection of the bladder neck; normal prostate-specific antigen (PSA) level < 4 ng/dL; and normal transrectal ultrasonography with biopsy of any suspicious nodes. Patients received complete oncological and functional follow-ups. The Kaplan-Meier method was utilized to characterize survival outcomes after surgery.

Results

The median follow-up times for PCS and NS were 144 and 122 months, respectively. Cumulative survival estimated the 5- and 10-years cancer-specific survival were 93.0% and 88.7% for the PCS group and 79.7% and 79.6% for the NS group, respectively (p = 0.123). In terms of function, the daytime urinary control at 3, 6, and 12 months postoperatively was 80.60%, 97.01%, and 100% in the PCS group, and 53.70%, 85.19%, and 94.44% in the NS group, respectively (p = 0.002, 0.023, and 0.100); and nocturnal urinary control was 62.69%, 94.03%, and 98.51% in the PCS group, and 40.74%, 72.22%, and 87.04% in the NS group, respectively (p = 0.016, 0.001, and 0.022). The erectile function recovery revealed that 62.69% and 40.74% of patients returned to preoperative levels (International Index of Erectile Function (IIEF)-5 score ≥ 15) in the PCS and NS groups, respectively (p = 0.016). Considering complications within 30 days after surgery, 4.48% and 7.69% patients had Clavien ≥ III complications in the PCS and NS groups, respectively (p = 0.700).

Conclusion

The PCS provides better restored urinary control and sexual function than the NS technique and does not affect oncological outcomes. However, PCS is prone to bladder-neck obstruction complications and requires closer long-term follow-up.
“Building” Human Health: When Doctors and Physicists Work Together
Authors: Debad Susan J. , Kowalska Magdalena
Published: May 2024

In this article, we explore the importance of cooperation in science. Just as various construction trades must work together to build a skyscraper, scientists from separate fields can cooperate to tackle complex scientific challenges. This is called interdisciplinary collaboration, and it is a great way to do science. By bringing together knowledge and tools from multiple fields, scientists can uncover creative solutions and make meaningful connections that they might not have reached on their own. We give an example of how collaboration between particle physics and medicine—two fields that seem very different from one another—come together to improve healthcare. Using the tools of particle physics, scientists are enhancing cancer diagnosis and treatment. Interdisciplinary collaboration is the best way to address many of the complex issues we face today, like controlling climate change or fighting cancer, and it can help scientists and doctors make a lasting impact on human lives and the health of our planet.
Shaping the Future of Destinations: New Clues to Smart Tourism Research from a Neuroscience Methods Approach
Authors: Cardoso Lucília , Fraga Carla
Published: May 2024

In the context of the technological era, the smart tourism construct serves as a bridge between human and the artificial worlds, combining social sciences and neurosciences. This study aims to explore smart tourism through neuroscientific methods in order to shape the future of tourism destinations, using a hybrid methodology combining bibliometric techniques and content analysis. The findings reveal the integration of diverse scientific domains, highlighting a transdisciplinary approach. They offer clear evidence that neuroscientific methods in smart tourism integrate multiple areas of scientific knowledge, surpassing disciplinary boundaries. “Destination” stands out alongside “emotion”, “visual attention” and eye tracking (ET). The collaboration network reveals the emergence of a new school, called neurotourism in the 21st century, formed mainly by actors and organizations from the Global North, evoking the need to include the Global South in the research scenario. The predominant methods include ET, heart rate (HR), and electroencephalography (EEG), suggesting triangulation with traditional methods for robust results. Virtual reality emerges as the primary immersive technology, promising insights when integrated with neurosciences. This study’s practical and theoretical contributions guide smart tourism strategies and enhance destination experiences through neuroscientific methods, addressing a gap in the scientific literature while advancing ontological and epistemological understanding.
Optimization and Prediction of Operational Parameters for Enhanced Efficiency of a Chickpea Peeling Machine
Authors: Ali Khaled Abdeen Mousa , Li Sheng Tao , Li Changyou , Darwish Elwan Ali , Wang Han , Abdelwahab Taha Abdelfattah Mohammed , Fodah Ahmed Elsayed Mahmoud , Elsaadawi Youssef Fayez
Published: May 2024

Chickpeas hold significant nutritional and cultural importance, being a rich source of protein, fiber, and essential vitamins and minerals. They are a staple ingredient in various cuisines worldwide. Peeling chickpeas is considered a crucial pre-consumption operation due to the undesirability of peels for some uses. This study aimed to design, test, and evaluate a small chickpea seed peeling machine. The peeling prototype was designed in accordance with the chickpeas’ measured properties; the seeds’ moisture content was determined to be 6.96% (d.b.). The prototype was examined under four different levels of drum revolving speeds (100, 200, 300, and 400 rpm), and three different numbers of brush peeling rows. The prototype was tested with rotors of four, eight, and twelve rows of brushes. The evaluation of the chickpea peeling machine encompassed several parameters, including the machine’s throughput (kg/h), energy consumption (kW), broken seeds percentage (%), unpeeled seeds percentage (%), and peeling efficiency (%). The obtained results revealed that the peeling machine throughput (kg/h) exhibited an upward trend with increases in the rotation speed of the peeling drum. Meanwhile, the throughput decreased as the number of peeling brushes installed on the roller increased. The highest recorded productivity of 71.29 kg/h was achieved under the operational condition of 400 rpm and four peeling brush rows. At the same time, the peeling efficiency increased with the increase in both of peeling drum rotational speed and number of peeling brush rows. The highest peeling efficiency (97.2%) was recorded at the rotational speed of 400 rpm and twelve peeling brush rows. On the other hand, the lowest peeling efficiency (92.85%) was recorded at the lowest drum rotational speed (100 rpm) and number of peeling brush rows (4 rows). In the optimal operational condition, the machines achieved a throughput of 71.29 kg/h, resulting in a peeling cost of 0.001 USD per kilogram. This small-scale chickpea peeling machine is a suitable selection for small and medium producers.
Unraveling the Major Determinants behind Price Changes in Four Selected Representative Agricultural Products
Authors: Tandogan Aktepe Nisa Sansel , Kayral İhsan Erdem
Published: May 2024

This study aims to analyze the drivers behind price changes in agricultural products in Türkiye from 2002 to 2021, considering the impacts of three crises of different causes which are the global food crisis, the Russia–Türkiye aircraft crisis, and the COVID-19 pandemic. The potential factors are categorized into four subgroups: governmental effects, agricultural inputs, macroeconomic indicators, and climatic conditions. The selected agricultural goods for price change measurement include wheat and maize representing subsistence goods, and olive oil and cotton as marketing goods. The autoregressive distributed lag (ARDL) model is applied to observe both the short- and long-term impacts of the variables on price developments. The results suggest that government effectiveness, regulatory quality, nitrogen use, water price, money supply, exchange rate, and GDP under the related categories are the most effective factors in price changes. Among the variables under the category of climatic conditions, significant values are obtained only in the analysis of the temperature impact on olive oil. The analysis also reveals the variable impact of crises on the prices of the chosen products, depending on the goods involved. The maize and wheat analyses yield particularly noteworthy results. In the long run, nitrogen use demonstrates a substantial positive impact, registering at 29% for wheat and 19.47% for maize, respectively. Conversely, GDP exhibits a significant negative impact, with 26.15% and 20.08%. Short-term observations reveal that a unit increase in the governmental effect leads to a reduction in inflation for these products by 17.01% and 21.42%. However, changes in regulatory quality result in an increase in inflation by 25.45% and 20.77% for these products, respectively.
The Impact of Agricultural Socialized Service on Grain Production: Evidence from Rural China
Authors: Li Ruisheng , Chen Jiaoyan , Xu Dingde
Published: May 2024

Although China’s grain production has reached nineteen consecutive harvests, the uncertainty of the current domestic and international environment has put more pressure on further increasing grain production in the future. For the past few years, agricultural socialization services have been crucial in boosting grain production and farmers’ revenue by addressing the issue of land cultivation and farming methods. In this regard, the question of whether and how agricultural socialized services may resolve the present grain production conundrum is extremely practical. Therefore, the study employs the China Rural Revitalization Survey data of 3709 households. Based on the 2SLS model, stepwise regression method, and moderated effects model, it creatively takes into account a variety of agricultural production segments, investigates the mechanism of services on grain production from the standpoint of improved production efficiency and plot concentration, and further examines the effects of aging populations and regional variations in grain production areas. The study found the following: (1) The average proportion of grain production area of farmers in the sample is 49%, and 42% of farmers have purchased agricultural socialization services. (2) Agricultural socialization services can significantly promote farmers’ grain cultivation behavior by facilitating connected transfers in and inhibiting connected transfers out to take advantage of plot concentration, and boosting the use of agricultural machines to enhance output efficiency. (3) The aging of the agricultural population will, to a certain extent, strengthen the promoting effect of agricultural socialization services on grain cultivation. Agricultural socialization services affect grain cultivation more in main grain-producing areas. Therefore, emphasizing the role of agricultural socialization services in accelerating the shift to moderate-scale operations, decreasing the non-grain component of the planting structure, and promoting the implementation of policies tailored to actual production needs are important steps to safeguard the production capacity of grain in different regions.
Assessment of Resistance of Barley Varieties to Diseases in Polish Organic Field Trials
Authors: Lenartowicz Tomasz , Bujak Henryk , Przystalski Marcin , Mashevska Inna , Nowosad Kamila , Jończyk Krzysztof , Feledyn-Szewczyk Beata
Published: May 2024

Leaf rust and net blotch are two important fungal diseases of barley. Leaf rust is the most important rust disease of barley, whereas net blotch can result in significant yield losses and cause the deterioration of crop quality. The best and the most environmentally friendly method to control diseases is to cultivate resistant varieties. The aim of the current study was to identify barley varieties with an improved resistance to leaf rust and net blotch in Polish organic post-registration trials conducted in the years 2020–2022. For this purpose, the cumulative link mixed model with several variance components was applied to model resistance to leaf rust and net blotch. It was found that the reference variety Radek was the most resistant to leaf rust, whereas variety Avatar outperformed the reference variety in terms of resistance to net blotch, although the difference between the two varieties was non-significant. In the present study, the use of the cumulative link mixed model framework made it possible to calculate cumulative probabilities or the probability of a given score for each variety and disease, which might be useful for plant breeders and crop experts. Both, the method of analysis and resistant varieties may be used in the breeding process to derive new resistant varieties suitable for the organic farming system.
A Study of Farmers’ Behavior in Classifying Domestic Waste Based on the Participants Intellectual Decision Model
Authors: Wang Jing , Zhao Nan , Li Dongjian , Li Shiping
Published: May 2024

The farmers’ deep participation in the classification of domestic waste plays a crucial role in reducing the amount of waste out of the village from the source, lowering the cost of waste treatment, and realizing the sustainable development of rural waste resocialization, reduction, and harmlessness. This paper aims to identify the key factors and logical structure that influence the farmers’ behavior in classifying domestic waste and provide recommendations for improving it. Based on the Participants’ Intellectual Decision (PID) Model, we constructed a theoretical analysis framework for farmers’ decision-making on domestic waste classification, and the PID model was further extended by combining with the practice of rural domestic waste management in China and proposing the research hypothesis that factors, such as community attributes, rules of operation, the status of the participants, and the situation of external actions, have a significant impact on the farmers’ behavior in classifying domestic waste. Empirical analyses were carried out with the help of the ordered logistic model and the DEMATEL-ISM using 939 research data of farmers in Jiangsu and Gansu provinces of China. The results show the following: (1) classification of domestic waste by farmers in the sample area was predominantly unclassified (34.40%) and two-classified (40.58%); (2) 17 factors, including regional disparity, Party affiliation, organizational support perception, environmental emotions, conscious governance attitudes, trust in village cadres, social reference norms, and expected outcomes, have a significant impact on the farmers’ behavior in classifying domestic waste; (3) trust in village cadres, organizational support perception, and environmental emotion are superficial direct factors; incentive measures, fee level, waste transport situation, difficulty perception, self-consciousness perception, social reference norms, and expected outcomes are middle indirect factors; whether or not it is a demonstration village, Party membership and regional disparity are deep root factors affecting farmers to classify their domestic waste.
The Triterpenoid CDDO-Methyl Ester Reduces Tumor Burden, Reprograms the Immune Microenvironment, and Protects from Chemotherapy-Induced Toxicity in a Preclinical Mouse Model of Established Lung Cancer
Authors: Moerland Jessica A. , Liby Karen T.
Published: May 2024

NRF2 activation protects epithelial cells from malignancy, but cancer cells can upregulate the pathway to promote survival. NRF2 activators including CDDO-Methyl ester (CDDO-Me) inhibit cancer in preclinical models, suggesting NRF2 activation in other cell types may promote anti-tumor activity. However, the immunomodulatory effects of NRF2 activation remain poorly understood in the context of cancer. To test CDDO-Me in a murine model of established lung cancer, tumor-bearing wildtype (WT) and Nrf2 knockout (KO) mice were treated with 50–100 mg CDDO-Me/kg diet, alone or combined with carboplatin/paclitaxel (C/P) for 8–12 weeks. CDDO-Me decreased tumor burden in an Nrf2-dependent manner. The combination of CDDO-Me plus C/P was significantly (p < 0.05) more effective than either drug alone, reducing tumor burden by 84% in WT mice. CDDO-Me reduced the histopathological grade of WT tumors, with a significantly (p < 0.05) higher proportion of low-grade tumors and a lower proportion of high-grade tumors. These changes were augmented by combination with C/P. CDDO-Me also protected WT mice from C/P-induced toxicity and improved macrophage and T cell phenotypes in WT mice, reducing the expression of CD206 and PD-L1 on macrophages, decreasing immunosuppressive FoxP3+ CD4+ T cells, and increasing activation of CD8+ T cells in a Nrf2-dependent manner.
A Novel MAO-B/SSAO Inhibitor Improves Multiple Aspects of Dystrophic Phenotype in mdx Mice
Authors: Gasparella Francesca , Nogara Leonardo , Germinario Elena , Tibaudo Lucia , Ciciliot Stefano , Piccoli Giorgia , Venegas Francisca Carolina , Fontana Francesca , Sales Gabriele , Sabbatini Daniele , Foot Jonathan , Jarolimek Wolfgang , Blaauw Bert , Canton Marcella , Vitiello Libero
Published: May 2024

Duchenne muscular dystrophy (DMD) is one of the most frequent and severe childhood muscle diseases. Its pathophysiology is multifaceted and still incompletely understood, but we and others have previously shown that oxidative stress plays an important role. In particular, we have demonstrated that inhibition of mitochondrial monoamine oxidases could improve some functional and biohumoral markers of the pathology. In the present study we report the use of dystrophic mdx mice to evaluate the efficacy of a dual monoamine oxidase B (MAO-B)/semicarbazide-sensitive amine oxidase (SSAO) inhibitor, PXS-5131, in reducing inflammation and fibrosis and improving muscle function. We found that a one-month treatment starting at three months of age was able to decrease reactive oxygen species (ROS) production, fibrosis, and inflammatory infiltrate in the tibialis anterior (TA) and diaphragm muscles. Importantly, we also observed a marked improvement in the capacity of the gastrocnemius muscle to maintain its force when challenged with eccentric contractions. Upon performing a bulk RNA-seq analysis, PXS-5131 treatment affected the expression of genes involved in inflammatory processes and tissue remodeling. We also studied the effect of prolonged treatment in older dystrophic mice, and found that a three-month administration of PXS-5131 was able to greatly reduce the progression of fibrosis not only in the diaphragm but also in the heart. Taken together, these results suggest that PXS-5131 is an effective inhibitor of fibrosis and inflammation in dystrophic muscles, a finding that could open a new therapeutic avenue for DMD patients.
Impact of Relative Biologic Effectiveness for Proton Therapy for Head and Neck and Skull-Base Tumors: A Technical and Clinical Review
Authors: Holtzman Adam L. , Mohammadi Homan , Furutani Keith M. , Koffler Daniel M. , McGee Lisa A. , Lester Scott C. , Gamez Mauricio E. , Routman David M. , Beltran Chris J. , Liang Xiaoying
Published: May 2024

Proton therapy has emerged as a crucial tool in the treatment of head and neck and skull-base cancers, offering advantages over photon therapy in terms of decreasing integral dose and reducing acute and late toxicities, such as dysgeusia, feeding tube dependence, xerostomia, secondary malignancies, and neurocognitive dysfunction. Despite its benefits in dose distribution and biological effectiveness, the application of proton therapy is challenged by uncertainties in its relative biological effectiveness (RBE). Overcoming the challenges related to RBE is key to fully realizing proton therapy’s potential, which extends beyond its physical dosimetric properties when compared with photon-based therapies. In this paper, we discuss the clinical significance of RBE within treatment volumes and adjacent serial organs at risk in the management of head and neck and skull-base tumors. We review proton RBE uncertainties and its modeling and explore clinical outcomes. Additionally, we highlight technological advancements and innovations in plan optimization and treatment delivery, including linear energy transfer/RBE optimizations and the development of spot-scanning proton arc therapy. These advancements show promise in harnessing the full capabilities of proton therapy from an academic standpoint, further technological innovations and clinical outcome studies, however, are needed for their integration into routine clinical practice.
Tripartite Motif-Containing 2, a Glutamine Metabolism-Associated Protein, Predicts Poor Patient Outcome in Triple-Negative Breast Cancer Treated with Chemotherapy
Authors: Masisi Brendah K. , El Ansari Rokaya , Alfarsi Lutfi , Fakroun Ali , Erkan Busra , Ibrahim Asmaa , Toss Michael , Ellis Ian O. , Rakha Emad A. , Green Andrew R.
Published: May 2024

Background: Breast cancer (BC) remains heterogeneous in terms of prognosis and response to treatment. Metabolic reprogramming is a critical part of oncogenesis and a potential therapeutic target. Glutaminase (GLS), which generates glutamate from glutamine, plays a role in triple-negative breast cancer (TNBC). However, targeting GLS directly may be difficult, as it is essential for normal cell function. This study aimed to determine potential targets in BC associated with glutamine metabolism and evaluate their prognostic value in BC. Methods: The iNET model was used to identify genes in BC that are associated with GLS using RNA-sequencing data. The prognostic significance of tripartite motif-containing 2 (TRIM2) mRNA was assessed in BC transcriptomic data (n = 16,575), and TRIM2 protein expression was evaluated using immunohistochemistry (n = 749) in patients with early-stage invasive breast cancer with long-term follow-up. The associations between TRIM2 expression and clinicopathological features and patient outcomes were evaluated. Results: Pathway analysis identified TRIM2 expression as an important gene co-expressed with high GLS expression in BC. High TRIM2 mRNA and TRIM2 protein expression were associated with TNBC (p < 0.01). TRIM2 was a predictor of poor distant metastasis-free survival (DMFS) in TNBC (p < 0.01), and this was independent of established prognostic factors (p < 0.05), particularly in those who received chemotherapy (p < 0.05). In addition, TRIM2 was a predictor of shorter DMFS in TNBC treated with chemotherapy (p < 0.01). Conclusions: This study provides evidence of an association between TRIM2 and poor patient outcomes in TNBC, especially those treated with chemotherapy. The molecular mechanisms and functional behaviour of TRIM2 and the functional link with GLS in BC warrant further exploration using in vitro models.