bluuebunny/crossref_metadata_2025 huggingface dataset:
cPLA2 blockade attenuates S100A7-mediated breast tumorigenicity by inhibiting the immunosuppressive tumor microenvironment
Authors: Mishra Sanjay , Charan Manish , Shukla Rajni Kant , Agarwal Pranay , Misri Swati , Verma Ajeet K. , Ahirwar Dinesh K. , Siddiqui Jalal , Kaul Kirti , Sahu Neety , Vyas Kunj , Garg Ayush Arpit , Khan Anum , Miles Wayne O. , Song Jonathan W. , Bhutani Nidhi , Ganju Ramesh K.
Published: February 2022

Background

Molecular mechanisms underlying inflammation-associated breast tumor growth are poorly studied. S100A7, a pro-inflammatory molecule has been shown to enhance breast cancer growth and metastasis. However, the S100A7-mediated molecular mechanisms in enhancing tumor growth and metastasis are unclear.

Methods

Human breast cancer tissue and plasma samples were used to analyze the expression of S100A7, cPLA2, and PGE2. S100A7-overexpressing or downregulated human metastatic breast cancer cells were used to evaluate the S100A7-mediated downstream signaling mechanisms. Bi-transgenic mS100a7a15 overexpression, TNBC C3 (1)/Tag transgenic, and humanized patient-derived xenograft mouse models and cPLA2 inhibitor (AACOCF3) were used to investigate the role of S100A7/cPLA2/PGE2 signaling in tumor growth and metastasis. Additionally, CODEX, a highly advanced multiplexed imaging was employed to delineate the effects of S100A7/cPLA2 inhibition on the recruitment of various immune cells.

Results

In this study, we found that S100A7 and cPLA2 are highly expressed and correlate with decreased overall survival in breast cancer patients. Further mechanistic studies revealed that S100A7/RAGE signaling promotes the expression of cPLA2 to mediate its oncogenic effects. Pharmacological inhibition of cPLA2 suppressed S100A7-mediated tumor growth and metastasis in multiple pre-clinical models including transgenic and humanized patient-derived xenograft (PDX) mouse models. The attenuation of cPLA2 signaling reduced S100A7-mediated recruitment of immune-suppressive myeloid cells in the tumor microenvironment (TME). Interestingly, we discovered that the S100A7/cPLA2 axis enhances the immunosuppressive microenvironment by increasing prostaglandin E2 (PGE2). Furthermore, CO-Detection by indEXing (CODEX) imaging-based analyses revealed that cPLA2 inhibition increased the infiltration of activated and proliferating CD4+and CD8+T cells in the TME. In addition, CD163+tumor associated-macrophages were positively associated with S100A7 and cPLA2 expression in malignant breast cancer patients.

Conclusions

Our study provides new mechanistic insights on the cross-talk between S100A7/cPLA2 in enhancing breast tumor growth and metastasis by generating an immunosuppressive TME that inhibits the infiltration of cytotoxic T cells. Furthermore, our studies indicate that S100A7/cPLA2 could be used as novel prognostic marker and cPLA2 inhibitors as promising drugs against S100A7-overexpressing aggressive breast cancer.
Australian podiatrists scheduled medicine prescribing practices and barriers and facilitators to endorsement: a cross‐sectional survey
Authors: Graham Kristin , Matricciani Lisa , Banwell Helen , Kumar Saravana , Causby Ryan , Martin Saraid , Nissen Lisa
Published: February 2022

Background

Non‐medical prescribing is one healthcare reform strategy that has the potential to create health system savings and offer equitable and timely access to scheduled medicines. Podiatrists are well positioned to create health system efficiencies through prescribing, however, only a small proportion of Australian podiatrists are endorsed to prescribe scheduled medicines. Since scheduled medicines prescribed by Australian podiatrists are not subsidised by the Government, there is a lack of data available on the prescribing practices of Australian podiatrists. The aim of this research was to investigate the prescribing practices among Australian podiatrists and to explore barriers and facilitators that influence participation in endorsement.

Methods

Participants in this quantitative, cross‐sectional study were registered and practicing Australian podiatrists who were recruited through a combination of professional networks, social media, and personal contacts. Respondents were invited to complete a customised self‐reported online survey, developed using previously published research, research team's expertise, and was piloted with podiatrists. The survey contained three sections: demographic data including clinical experience, questions pertaining to prescribing practices, and barriers and facilitators of the endorsement pathway.

Results

Respondents (n = 225) were predominantly female, aged 25–45, working in the private sector. Approximately one quarter were endorsed (15%) or in training to become endorsed (11%). Of the 168 non‐endorsed respondents, 66% reported that they would like to undertake training to become an endorsed prescriber.

The most common indications reported for prescribing or recommending medications include nail surgery (71%), foot infections 474 (88%), post‐operative pain (67%), and mycosis (95%). The most recommended Schedule 2 medications were ibuprofen, paracetamol, and topical terbinafine. The most prescribed Schedule 4 medicines among endorsed podiatrists included lignocaine (84%), cephalexin (68%), flucloxacillin (68%), and amoxicillin with clavulanic acid (61%).

Conclusion

Podiatrists predominantly prescribe scheduled medicines to assist pain, inflammatory, or infectious conditions. Only a small proportion of scheduled medicines available for prescription by podiatrists with endorsed status were reportedly prescribed. Many barriers exist in the current endorsement for podiatrists, particularly related to training processes, including mentor access and supervised practice opportunities. Suggestions to address these barriers require targeted enabling strategies.
Multidimensional analysis of the host response reveals prognostic and pathogen-driven immune subtypes among adults with sepsis in Uganda
Authors: Cummings Matthew J. , Bakamutumaho Barnabas , Price Adam , Owor Nicholas , Kayiwa John , Namulondo Joyce , Byaruhanga Timothy , Muwanga Moses , Nsereko Christopher , Sameroff Stephen , Tokarz Rafal , Wong Wai , Shah Shivang S. , Larsen Michelle H. , Lipkin W. Ian , Lutwama Julius J. , O’Donnell Max R.
Published: February 2022

Background

The global burden of sepsis is concentrated in sub-Saharan Africa, where severe infections disproportionately affect young, HIV-infected adults and high-burden pathogens are unique. In this context, poor understanding of sepsis immunopathology represents a crucial barrier to development of locally-effective treatment strategies. We sought to determine inter-individual immunologic heterogeneity among adults hospitalized with sepsis in a sub-Saharan African setting, and characterize associations between immune subtypes, infecting pathogens, and clinical outcomes.

Methods

Among a prospective observational cohort of 288 adults hospitalized with suspected sepsis in Uganda, we applied machine learning methods to 14 soluble host immune mediators, reflective of key domains of sepsis immunopathology (innate and adaptive immune activation, endothelial dysfunction, fibrinolysis), to identify immune subtypes in randomly-split discovery (N = 201) and internal validation (N = 87) sub-cohorts. In parallel, we applied similar methods to whole-blood RNA-sequencing data from a consecutive subset of patients (N = 128) to identify transcriptional subtypes, which we characterized using biological pathway and immune cell-type deconvolution analyses.

Results

Unsupervised clustering consistently identified two immune subtypes defined by differential activation of pro-inflammatory innate and adaptive immune pathways, with transcriptional evidence of concomitant CD56(-)/CD16( +) NK-cell expansion, T-cell exhaustion, and oxidative-stress and hypoxia-induced metabolic and cell-cycle reprogramming in the hyperinflammatory subtype. Immune subtypes defined by greater pro-inflammatory immune activation, T-cell exhaustion, and metabolic reprogramming were consistently associated with a high-prevalence of severe and often disseminated HIV-associated tuberculosis, as well as more extensive organ dysfunction, worse functional outcomes, and higher 30-day mortality.

Conclusions

Our results highlight unique host- and pathogen-driven features of sepsis immunopathology in sub-Saharan Africa, including the importance of severe HIV-associated tuberculosis, and reinforce the need to develop more biologically-informed treatment strategies in the region, particularly those incorporating immunomodulation.
Unique ER PR expression pattern in breast cancers with CHEK2 mutation: a hormone receptor and HER2 analysis based on germline cancer predisposition genes
Authors: Wei Grace , Teng Mingxiang , Rosa Marilin , Wang Xia
Published: February 2022

Purpose

Estrogen-receptor (ER) and progesterone-receptor (PR) expression levels in breast cancer, which have been principally compared via binomial descriptors, can vary widely across tumors. We sought to characterize ER and PR expression levels using semi-quantitative analyses of receptor staining in germline pathogenic variant (PV) carriers of cancer predisposition genes.

Methods

We conducted a retrospective chart review of patients who underwent germline genetic testing for cancer predisposition genes at a tertiary cancer center genetics clinic. We performed comparisons of semi-quantitative ER and PR percentage staining levels across carriers and non-carriers of cancer predisposition genes.

Results

Breast cancers from BRCA1 PV carriers expressed significantly lower ER (15.2% vs 78.2%, p < 0.001) and lower PR (6.8% vs 41.1%, p < 0.001) staining compared to non-PV carriers. Similarly, breast cancers of BRCA2 (66.7% vs 78.2%, p = 0.005) and TP53 (50.6% vs 78.2%, p = 0.015) PV tumors also displayed moderate decreases in ER staining. Conversely, CHEK2 tumors displayed higher ER (93.1% vs 78.2%, p = 0.005) and PR (72% vs 48.8%, p = 0.001) staining when compared to non-PV carriers. We observed a wide range of dispersion across the ER and PR staining levels of the carriers and noncarriers. ER and PR ranges of dispersion of CHEK2 tumors were uniquely narrower than all other groups.

Conclusion

The findings of our study suggest that precise expression levels of ER and PR in breast cancers can vary widely. These differences are further augmented when comparing expression staining across PV and non-PV carriers, suggesting potentially unique tumorigenesis and progression pathways influenced by germline cancer predisposition genes.
Assessing the effect of an educational intervention on early childhood development among Mexican preschool children in the state of Oaxaca: a study protocol of a cluster randomized stepped-wedge trial
Authors: Quezada-Sánchez Amado D. , Fuentes-Rivera Evelyn , García-Martínez Angélica , del Carmen Hernández-Chávez María , Pineda-Antúnez Carlos , Martínez Martín Romero , García-Guerra Armando , García-Feregrino Raquel , Madrigal-Ramírez Abby , Santiago-Angelino Tania , Olvera-Flores Fabián , Schnaas Lourdes , Pérez-Escamilla Rafael , Serván-Mori Edson
Published: February 2022

Background

Early childhood development (ECD) is essential in human capacity building and a critical element in the intergenerational process of human development. In some countries, social programs targeted at improving ECD have proven to be successful. Oaxaca is one of the States with the greatest social inequities in Mexico. Therefore, children in Oaxaca are at a high risk of suboptimal ECD. In 2014, the non-governmental organization (NGO) Un Kilo de Ayuda started to implement the Neurological and Psycho-affective Early Childhood Development Program in eighty marginalized communities of Oaxaca. In this article, we present the impact evaluation design to estimate the effect of this program on ECD.

Methods

We will use a cluster randomized stepped-wedge design with an allocation ratio of 1:1. Communities will be randomly assigned to each study group: four groups of twenty communities each. We expect that children from intervened communities will show better ECD outcomes.

Discussion

This study is one of the few rigorous assessments of the effect of an ECD program on the neurodevelopment of Mexican children recruited in their first 3 years of life from communities of high social vulnerability. Our study design is recommended when the way in which outcomes are measured and assessed depends on age, self-selection is present, and assignment is performed at an aggregate level. Implementation research will be conducted prior to study launch and quality control measures will be in place to maximize the fidelity of study design implementation.

Trial registration

ClinicalTrials.gov NCT04210362
Evaluation of the effectiveness of BG-Sentinel and CDC light traps in assessing the abundance, richness, and community composition of mosquitoes in rural and natural areas
Authors: Wilke André B. B. , Vasquez Chalmers , Carvajal Augusto , Moreno Maday , Petrie William D. , Beier John C.
Published: February 2022

Background

Vector-borne diseases are a major burden to public health. Controlling mosquitoes is considered the most effective way to prevent vector-borne disease transmission. Mosquito surveillance is a core component of integrated vector management, as surveillance programs are often the cornerstone for the development of mosquito control operations. Two traps are the most commonly used for the surveillance of adult mosquitoes: Centers for Disease Control and Prevention miniature light trap (CDC light trap) and BG-Sentinel trap (BioGents, Regensburg, Germany). However, despite the importance of the BG-Sentinel trap in surveillance programs in the United States, especially in the Southern states, its effectiveness in consistently and reliably collecting mosquitoes in rural and natural areas is still unknown. We hypothesized that BG-Sentinel and CDC light traps would be more attractive to specific mosquito species present in rural and natural areas. Therefore, our objective was to compare the relative abundance, species richness, and community composition of mosquitoes collected in natural and rural areas by BG-Sentinel and CDC light traps.

Methods

Mosquitoes were collected from October 2020 to March 2021 using BG-Sentinel and CDC light traps baited with dry ice, totaling 105 trap-nights.

Results

The BG-Sentinel traps collected 195,115 mosquitoes comprising 23 species from eight genera, and the CDC light traps collected 188,594 mosquitoes comprising 23 species from eight genera. The results from the permutational multivariate analysis of variance (PERMANOVA) and generalized estimating equation model for repeated measures indicate the BG-Sentinel and CDC light traps had similar sampling power.

Conclusion

Even though BG-Sentinel traps had a slightly better performance, the difference was not statistically significant indicating that both traps are suitable to be used in mosquito surveillance in rural and natural areas.

Graphical Abstract
Time-course effects of functional fitness sessions performed at different intensities on the metabolic, hormonal, and BDNF responses in trained men
Authors: Tibana Ramires Alsamir , de Sousa Neto Ivo Vieira , de Sousa Nuno Manuel Frade , dos Santos Wellington Martins , Prestes Jonato , Neto João Henrique Falk , Dominski Fábio H. , Kennedy Michael D. , Voltarelli Fabricio Azevedo
Published: February 2022

Background

To investigate the time-course effects of a self-regulated training session (performed at an rating perceived exertion of 6/10), all-out session, and a control session on the metabolic, hormonal, and brain derived neurotrophic factor (BDNF) responses in Functional-Fitness (FFT) participants.

Methods

In a randomized, crossover fashion, eight healthy males (age 28.1 ± 5.4 years old; body mass 77.2 ± 4.4 kg; VO2max: 52.6 ± 4.6 mL.(kg.min)−1; 2000 m rowing test 7.35 ± 0.18 min; 1RM back squat 135.6 ± 21.9 kg) performed a FFT session under two different conditions: all-out, or with the intensity controlled to elicit an rating perceived exertion (RPE) of 6 in the Borg 10-point scale (RPE6). A control session (no exercise) was also completed. Metabolic (lactate and creatine kinase), hormonal (testosterone and cortisol), and BDNF responses were assessed pre, post-0 h, 1 h, 2 h and 24 h after the sessions.

Results

Creatine kinase concentrations were significantly higher (p ≤ 0.05) after 24 h for both training sessions. Total and free testosterone concentrations were lower post-2 h for all-out when compared to the RPE6 session (p ≤ 0.05). Serum cortisol concentration increased post-0 h (p = 0.011) for RPE6 and post-0 h (p = 0.003) and post-1 h (p = 0.030) for all-out session when comparing to baseline concentrations. BDNF was significantly higher (p = 0.002) post-0 h only for the all-out session when compared to baseline. A positive correlation between blood lactate concentrations and BDNF (r = 0.51; p = 0.01) was found for both effort interventions.

Conclusions

A single FFT session when performed in all-out format acutely increases the concentrations of serum BDNF. However, physiological stress markers show that the all-out session requires a longer recovery period when compared to the RPE6 protocol. These findings can be helpful to coaches and practitioners design FFT session.
Does predicted age at peak height velocity explain physical performance in U13–15 basketball female players?
Authors: Gryko Karol , Adamczyk Jakub Grzegorz , Kopiczko Anna , Calvo Jorge Lorenzo , Calvo Alberto Lorenzo , Mikołajec Kazimierz
Published: February 2022

Background

The aims of the study were (1) to identify the physical fitness and basic anthropometric characteristics of Polish female basketball players aged 13–15 years, (2) to show the effect of maturity timing on the performance in motor tests and basic body composition parameters, (3) to identify the index that contributes most to the prediction of performance in the tests of speed, jumping ability, agility, and endurance.

Methods

The sample included 904 female Polish players (U13–15). In part 1, maturity timing category distribution were examined within across age-groups. Maturity timing was followed by grouping with respect to years before or after the observed peak high velocity (PHV): PHV0 (− 0.50 to 0.49), PHV1 (0.50 to 1.49), PHV2 (1.50 to 2.49) and PHV3 (2.50 to 3.49). In part 2, the relationship between the anthropometric variables, physical fitness performance was assessed based on maturity timing categories (ANCOVA analysis). In part 3, backward stepwise multiple regression analyse quantified the relationship between maturity timing (group of PHV) and physical performance.

Results

ANCOVA results (age, body height, and body mass as covariates) showed in the U13 female basketball players significantly higher sprinting (20 m), jumping ability and endurance tests results of the PHV1 group. Better results was observed in U14 female players in PHV1 compared to PHV2 and PHV3 in 20 m and jumping tests but opposite trend was observed for 5 m sprint and endurance test (distance covered and VO2max). U15 basketball players from the PHV3 group were characterized by better results of jumping abilities, endurance, 10 m and 20 m sprint and agility (total, S4) tests. Maturity timing (10 m), chronological age (5 m, 20 m, agility, SVJ, VJ, and VO2maxtests), body height (10 m), body mass (10 m, 20 m, VJ, VO2max), and the interaction between body mass and height (SVJ) were significant (adjustedR2 = 0.02–0.10;p < 0.001) predictors of motor skills.

Conclusion

Trainng content of female basketball players aged 13–15 years old should be adjusted to biological requirements especially in jumping, endurance and 20 m sprint test. The time from peak height velocity (PHV) was a significant predictor only in the 10 m sprint test.
Abnormal Schwannoma-like Growth of multiple, multifocal BRAF V600E-positive Glioblastoma in the Interior Acoustic Canal with Leptomeningeal Infiltration: a case report
Authors: Jayarama-Naidu Roopa , Gallus Evelyn
Published: February 2022

Background

Glioblastoma belongs to the most common and most aggressive tumor entity of the central nervous system with a poor prognosis of only few months. Once manifested, it grows fast and diffusely by infiltrating the surrounding brain parenchyma. Despite its aggressive behavior, glioblastoma rarely presents with multiple lesions and metastasis to intra- and extracranial tissues. Therefore, metastasized, multiple glioblastoma is limited to case reports. Our case describes an atypical primary bilateral manifestation of BRAF V600E-positive epithelioid glioblastoma with rapid metastasis and meningeosis glioblastoma while under adjuvant chemoradiotherapy.

Case presentation

A 60-year-old Caucasian male patient presented with a seizure and numbness in his left arm. He was diagnosed with an abnormal primary bilateral manifestation of multiple, multifocal BRAF V600E-positive and isocitrate dehydrogenase (IDH) wild-type intracranial epithelioid glioblastoma with O6-methylguanine-DNA methyltransferase methylation (MGMT) at 12%. While being under the adjuvant chemoradiotherapy with temozolomide, the patient developed left-sided facial nerve weakness and hearing loss, dysarthria, and severe gait instability. Cranial magnetic resonance imaging showed that glioblastoma lesions advanced rapidly with a schwannoma-like growth pattern by invading the left internal acoustic meatus, adjacent cranial nerves, and leptomeninges. A lumbar puncture confirmed meningeosis glioblastoma. Four months after the initial diagnosis of glioblastoma, the patient died from the complications of the fast and diffuse metastasis.

Conclusions

Glioblastoma rarely presents with metastases despite its aggressive and rapidly growing nature. Our case should increase awareness of symptom tracking in patients with glioblastoma to intervene early and efficiently. Moreover, refractory therapies for glioblastoma should underline the importance of personalized medicine.
Synergistic regenerative therapy of thin endometrium by human placenta-derived mesenchymal stem cells encapsulated within hyaluronic acid hydrogels
Authors: Lin Yifeng , Dong Shunni , Ye Xiaohang , Liu Juan , Li Jiaqun , Zhang Yanye , Tu Mixue , Wang Siwen , Ying Yanyun , Chen Ruixue , Wang Feixia , Ni Feida , Chen Jianpeng , Du Binyang , Zhang Dan
Published: February 2022

Background

Thin endometrium is a primary cause of defective endometrial receptivity, resulting in infertility or recurrent miscarriage. Much effort has been devoted toward regenerating thin endometrium by stem cell-based therapies. The human placenta-derived mesenchymal stem cells (HP-MSCs) are emerging alternative sources of MSCs with various advantages. To maximize their retention inside the uterus, we loaded HP-MSCs with cross-linked hyaluronic acid hydrogel (HA hydrogel) to investigate their therapeutic efficacy and possible underlying mechanisms.

Methods

Ethanol was injected into the mice uterus to establish the endometrium-injured model. The retention time of HP-MSCs and HA hydrogel was detected by in vivo imaging, while the distribution of HP-MSCs was detected by immunofluorescence staining. Functional restoration of the uterus was assessed by testing embryo implantation rates. The endometrial morphological alteration was observed by H&E staining, Masson staining, and immunohistochemistry. In vitro studies were further conducted using EdU, transwell, tube formation, and western blot assays.

Results

Instilled HP-MSCs with HA hydrogel (HP-MSCs-HA) exhibited a prolonged retention time in mouse uteri than normal HP-MSCs. In vivo studies showed that the HP-MSCs-HA could significantly increase the gland number and endometrial thickness (P < 0.001, P < 0.05), decrease fibrous area (P < 0.0001), and promote the proliferation and angiogenesis of endometrial cells (as indicated by Ki67 and VEGF, P < 0.05, P < 0.05, respectively) in mice injured endometrium. HP-MSCs-HA could also significantly improve the embryo implantation rate (P < 0.01) compared with the ethanol group. Further mechanistic study showed the paracrine effects of HP-MSCs. They could not only promote the proliferation and migration of human endometrial stromal cells via the JNK/Erk1/2-Stat3-VEGF pathway but also facilitate the proliferation of glandular cells via Jak2-Stat5 and c-Fos-VEGF pathway. In turn, the increased VEGF in the endometrium promoted the angiogenesis of endothelial cells.

Conclusion

Our study suggested the potential therapeutic effects and the underlying mechanisms of HP-MSCs-HA on treating thin endometrium. HA hydrogel could be a preferable delivery method for HP-MSCs, and the strategy represents a promising therapeutic approach against endometrial injury in clinical settings.

Graphical abstract
H3K27ac chromatin acetylation and gene expression analysis reveal sex- and situs-related differences in developing chicken gonads
Authors: Jiang Yunqi , Peng Zhelun , Man Qiu , Wang Sheng , Huang Xiaochen , Meng Lu , Wang Heng , Zhu Guiyu
Published: February 2022

Background

Birds exhibit a unique asymmetry in terms of gonad development. The female left gonad generates a functional ovary, whereas the right gonad regresses. In males, both left and right gonads would develop into testes. How is this left/right asymmetry established only in females but not in males remains unknown. The epigenetic regulation of gonadal developmental genes may contribute to this sex disparity. The modification of histone tails such as H3K27ac is tightly coupled to chromatin activation and gene expression. To explore whether H3K27ac marked chromatin activation is involved in the asymmetric development of avian gonads, we probed genome-wide H3K27ac occupancy in left and right gonads from both sexes and related chromatin activity profile to the expression of gonadal genes. Furthermore, we validated the effect of chromatin activity on asymmetric gonadal development by manipulating the chromatin histone acetylation levels.

Methods

The undifferentiated gonads from both sides of each sex were collected and subjected to RNA-Seq and H3K27ac ChIP-Seq experiments. Integrated analysis of gene expression and active chromatin regions were performed to identify the sex- and situs-specific regulation and expression of gonadal genes. The histone deacetylase inhibitor trichostatin A (TSA) was applied to the undifferentiated female right gonads to assess the effect of chromatin activation on gonadal gene expression and cell proliferation.

Results

Even before sex differentiation, the gonads already show divergent gene expression between different sexes and between left/right sides in females. The sex-specific H3K27ac chromatin distributions coincide with the higher expression of male/female specification genes in each sex. Unexpectedly, the H3K27ac marked chromatin activation show a dramatic difference between left and right gonads in both sexes, although the left/right asymmetric gonadal development was observed only in females but not in males. In females, the side-specific H3K27ac occupancy instructs the differential expression of developmental genes between the pair of gonads and contributes to the development of left but not right gonad. However, in males, the left/right discrepancy of H3K27ac chromatin distribution does not drive the side-biased gene expression or gonad development. The TSA-induced retention of chromatin acetylation causes up-regulation of ovarian developmental genes and increases cell proliferation in the female right gonad.

Conclusions

We revealed that left/right asymmetry in H3K27ac marked chromatin activation exists in both sexes, but this discrepancy gives rise to asymmetric gonadal development only in females. Other mechanisms overriding the chromatin activation would control the symmetric development of male gonads in chicken.
Sterilization of paper during crisis
Authors: Alshammari Fwzah H. , Hussein Hebat-Allah A.
Published: February 2022

Paper sheets represent one of the infection risk sources inside educational and administrative institutions under biological pandemics. So, the present study aimed to validate the efficiency of gamma radiation or dry heat techniques to sterilize contaminated paper sheets with different indicator pathogens while retaining their structure. The results showed that gamma radiation at 6, 12, or 24 kGy can successfully kill Gram-positive bacteria such as Bacillus cereus and Staphylococcus aureus, Gram-negative bacteria such as Escherichia coli and Salmonella typhi, and fungi such as Candida albicans. Moreover, dry heating at 100 °C for 60 min, 150 °C for 30 min, or 200 °C for 15 min can be successful in paper decontamination of all tested species. Surprisingly, scanning electron microscopy (SEM) micrographs proved that gamma radiation at 6 kGy, dry heat at 100 °C for 60 min or 150 °C for 30 min or 200 °C for 15 min, is suitable for paper sheet sterilization while maintaining their structure. Ultimately, dry heat is a simple, effective, fast, safe, and inexpensive technique for paper sterilization. It may be used as a precautionary step inside educational institutions, especially during written examination periods, to ensure a safe life for academic members during biological pandemics such as COVID-19.
Caring for the health and well-being of our learners in medicine as critical actions toward high-quality care
Authors: Karnieli-Miller Orit
Published: February 2022

A recent paper has focused on residents' poor lifestyle habits and their potential negative impact on patients' lifestyles. This commentary argues that there are even greater reasons to be concerned about the health and well-being of residents and medical students and the resultant effects on patients throughout the careers of these learners. There is a “hidden curriculum”, i.e., customs, rituals and norms of behavior, in medical education and in the training at the healthcare setting, often contradicts the formal curriculum and include messages that neglect the basic needs of the learners as well as the patients. Due to the impact of these messages on the professional identity formation of learners, including a deterioration in their own wellbeing as well as impairment of their ability to empathize with and care for patients, we must align our formal and hidden curricula to show dignity and caring for learners, colleagues, and patients. To do this well, we need to change our approach. We need to add processes for learners to support them in dealing with the stresses of their education and training and allow them to build their families and commit fully to medicine as a vocation, not just a job. We also must add faculty development processes to help align the formal and hidden curricula and help faculty empower and constructively assist their learners to handle challenging situations, e.g., where they see a resident struggling with patient care and day-to-day workload, through empathic feedback. When our learners are treated with kindness and respect they will lead more fulfilling lives and be better able to provide the high-quality care and caring all patients deserve.
Effect of a multimodal strategy for prevention of nosocomial influenza: a retrospective study at Grenoble Alpes University Hospital from 2014 to 2019
Authors: Gallouche Meghann , Terrisse Hugo , Larrat Sylvie , Marfaing Sylvie , Di Cioccio Christelle , Verit Bruno , Morand Patrice , Bonneterre Vincent , Bosson Jean-Luc , Landelle Caroline
Published: February 2022

Background

A multimodal strategy to prevent nosocomial influenza was implemented in 2015–2016 in Grenoble Alpes University Hospital. Three modalities were implemented in all units: promotion of vaccination among healthcare workers, epidemiologic surveillance and communication campaigns. Units receiving a high number of patients with influenza implemented 2 additional modalities: improvement of diagnosis capacities and systematic surgical mask use. The main objective was to assess the effectiveness of the strategy for reducing the risk of nosocomial influenza.

Methods

A study was conducted retrospectively investigating 5 epidemic seasons (2014–2015 to 2018–2019) including all patients hospitalized with a positive influenza test at Grenoble Alpes University Hospital. The weekly number of nosocomial influenza cases was analyzed by Poisson regression and incidence rate ratios (IRR) were estimated.

Results

A total of 1540 patients, resulting in 1559 stays, were included. There was no significant difference between the 5 influenza epidemic seasons in the units implementing only 3 measures. In the units implementing the 5 measures, there was a reduction of nosocomial influenza over the seasons when the strategy was implemented compared to the 2014–2015 epidemic season (IRR = 0.56, 95% CI = 0.23–1.34 in 2015–2016; IRR = 0.39, 95% CI = 0.19–0.81 in 2016–2017; IRR = 0.50, 95% CI = 0.24–1.03 in 2017–2018; IRR = 0.48, 95% CI = 0.23–0.97 in 2018–2019).

Conclusions

Our data mainly suggested that the application of the strategy with 5 modalities, including systematic surgical mask use and rapid diagnosis, seemed to reduce by half the risk of nosocomial influenza. Further data, including medico-economic studies, are necessary to determine the opportunity of extending these measures at a larger scale.
A qualitative study on safety perception among healthcare workers of a tertiary academic care center during the SARS-CoV-2 pandemic
Authors: Ehrenzeller Selina , Durovic Ana , Kuehl Richard , Martinez Aurélien Emmanuel , Bielser Michèle , Battegay Manuel , von Rotz Matthias , Fringer André , Tschudin-Sutter Sarah
Published: February 2022

Background

Protecting healthcare workers (HCWs) from exposure to SARS-CoV-2 during patient care is central to managing the current pandemic. Higher levels of trust in personal protective equipment (PPE) and infection prevention and control (IPC) strategies have been previously related to lower levels of emotional exhaustion, yet little is known on how to achieve such a perception of safety. We thus sought to identify institutional actions, strategies and policies related to HCWs’ safety perception during the early phase of the pandemic at a tertiary care center in Switzerland by interviewing HCWs from different clinics, professions, and positions.

Methods

For this qualitative study, 36 face-to-face semi-structured interviews were performed. Interviews were based on a guide that addressed the perception of institutional strategies and policies during the first phase of the pandemic in March 2020. The participants included doctors (n = 19) and nurses (n = 17) in senior and non-senior positions from eight clinics in the University Hospital Basel, Switzerland, all involved in patient care. All interviews were audio-recorded and transcribed verbatim. Data were analyzed using qualitative content analysis and organized using MAXQDA (VERBI Software GmbH, Berlin).

Findings

Five recurring themes were identified to affect HCWs’ perception of their safety during the SARS-CoV-2 pandemic: (1) transparency and clarity of information, (2) communication on the availability of PPE (with the provision of information alone increasing the feeling of safety even if supplies of PPE were reported as low), (3) uniformity and consistency of guidelines, (4) digital resources to support face-to-face teaching (although personal information transfer is still being considered superior in terms of strengthening safety perception) and (5) support and appreciation for the work performed.

Conclusions

This study identifies institutional policies and actions influencing HCWs’ safety perception during the first wave of the COVID-19 pandemic, the most important of which is the factor of transparent communication. This knowledge reveals potential areas of action critical to improving preparedness and management in hospitals faced with an infectious disease threat.
The simultaneous impact of access to credit and cooperative services on cocoa productivity in South-western Nigeria
Authors: Kehinde Ayodeji Damilola , Ogundeji Abiodun Akintunde
Published: February 2022

Background

Cocoa contributes immensely to Nigeria’s economy; it is about 40% of agricultural exports and it is the main source of livelihood for over 200,000 rural households. However, its productivity has remained low in recent years compared to other cocoa-producing countries such as Cote d’Ivoire and Ghana. Low cocoa productivity is attributed to many factors, which include lack of access to credit and cooperative services. However, empirical information on the simultaneous impact of credit access and membership of cooperative society on cocoa productivity is still very scanty. This paper aims at evaluating the impacts of access to credit service, cooperative service, and simultaneous access to credit and cooperative services on cocoa productivity in South-western Nigeria.

Method

A multistage sampling procedure was employed to select 300 cocoa farmers for the study. The endogenous switching regression (ESRM) and the inverse probability-weighted regression adjustment (IPWRA) models were used to analyse the data.

Results

In terms of simultaneous access to credit and cooperative services, age, education, gender, and size of household significantly influence the probability of farmers having simultaneous access to credit and cooperative services. The results of the second stage of the ESRM showed that age, size of household, years of education, and years of experience significantly influence cocoa productivity among farmers who have access to credit and cooperative services. However, age, size of household, size of farm and asset significantly influence the productivity of farmers who do not have access to credit and cooperative services. Average treatment effect on the treated (ATT) revealed that farmers who simultaneously have access to credit and cooperative services achieve significantly higher productivity than farmers who have access to either credit or cooperative services and the set of farmers who do not have access to either credit or cooperative services.

Conclusion

The study concluded that simultaneous access to credit and cooperative services positively impacted cocoa productivity. Farmers who have simultaneous access to credit and cooperative services achieve significantly higher productivity than farmers who have access to either credit or cooperative services or those that do not have access to these services at all. The study recommends that any agricultural productivity-targeted programmes in Nigeria consider farmers’ simultaneous access to credit and cooperative services.
Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells
Authors: Zhou Xiaoge , Mo Zhaoyi , Li Yankun , Huang Liang , Yu Sihai , Ge Lan , Hu Yamei , Shi Shengjie , Zhang Lutong , Wang Liguang , Gao Lei , Yang Gongshe , Chu Guiyan
Published: February 2022

Background

Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state, and it may have detrimental effects on female reproduction and fertility. Oleic acid induces lipid accumulation in bovine granulosa cells, which leads to a foam cell-like morphology and reduced steroidogenesis. However, why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear. This study focused on oleic acid’s effects on lipid type and steroidogenesis.

Results

Oleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester level. Oleic acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in vitro. In addition, oleic acid up-regulated the expression of CD36 and diacylglycerol acyltransferase 2, but down-regulated the expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2, as well as steroidogenesis-related genes, including cytochrome P450 family 11 subfamily A member 1, cytochrome P450 family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein levels. An oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice, which resulted in lower estradiol levels than in control-fed mice. Compared with the control, decreases in estrus days and the numbers of antral follicles and corpora lutea, as well as an increase in the numbers of the atretic follicles, were found in the oleic acid-fed female mice.

Conclusions

Oleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells, and led to a decrease in steroidogenesis. These results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.
Effects of in ovo delivered xylo- and mannan- oligosaccharides on growth performance, intestinal immunity, cecal short-chain fatty acids, and cecal microbiota of broilers
Authors: Singh Amit Kumar , Tiwari Utsav Prakash , Mishra Birendra , Jha Rajesh
Published: February 2022

Background

This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo- and mannan- oligosaccharides (XOS and MOS) differing in the degree of polymerization. A total of 192 fertilized eggs were divided into 6 treatment groups: i) normal saline control (NSC), ii) xylotriose (XOS3), iii) xylotetraose (XOS4), iv) mannotriose (MOS3), v) mannotetraose (MOS4), and vi) no injection control (NIC), each containing 4 replicate trays with 8 eggs per replicate. On d 17 of incubation, 3 mg of oligosaccharides (except for controls) dissolved in 0.5 mL of 0.85% normal saline were injected into the amnion of Cobb 500 broilers eggs. After hatch, the chicks were raised for 28 d under standard husbandry practices and were fed a commercial broilers diet ad libitum, and samples were collected periodically.

Results

The hatchability, growth performance, and relative weights of breast, drumstick, liver, and proventriculus were not different among the treatments (P > 0.05). The XOS3 injection increased the total short-chain fatty acid production at d 28 compared with both control groups (P < 0.05). The villus height to crypt depth ratio was significantly higher in the XOS4 group than both controls on the hatch day (P < 0.01) but were not different among any treatments on d 7 and 28 (P > 0.05). On the hatch day, the expression level of the CD3 gene (a T cell marker) was increased by XOS3, while the IL-10 gene (a marker of anti-inflammatory cytokine) was reduced by MOS4 (P < 0.05) compared with both controls. Compared with both controls, XOS3 exhibited a trend of reduction for IL-10 (P = 0.074). No cytokines or lymphocyte markers were affected by the treatments on d 7 (P > 0.05), except XOS4 increased IL-4 compared with NSC (P < 0.05). The broilers in the MOS4 group had higher operational taxonomic units (OTUs) and had more differentially abundant taxa, including order Lactobacillales and family Leuconostocaceae (P < 0.05) than both controls on d 28. The predictive functional profiling indicated that the linoleic acid metabolism pathway was enriched in the cecal microbiota of the XOS3 group compared with both controls (P < 0.05).

Conclusion

The effects of these XOS and MOS on ileal mucosa and immunity are transient, but the effects on fermentation and cecal microbiota are prolonged, and further research is warranted to determine their use as a gut health promoter in poultry.
A case study on Hoeamsa Temple, Korea: technical examination and identification of pigments and paper unearthed from the temple site
Authors: Go InHee , Mun SeongWoo , Lee JangJon , Jeong HyeYoung
Published: February 2022

This study applied various scientific analyses to one fragment of paper and three pigments excavated from earthenware found in the Hoeamsa Temple site at Yangju, Korea, which is believed to have been built in the early twelfth century. Radiocarbon dating of the paper fragment suggests a manufacture date between 1460 and 1646 (at a 95% confidence interval). It was estimated to have been used during the early and middle period of the Joseon dynasty (1391–1776), when the Hoeamsa Temple site was rebuilt. In addition, by staining the fiber of the paper fragment with Graff “C” stain, the paper’s raw material was identified as paper mulberry through dislocation, cross-marking, and transparent membrane, which are characteristics of the bast fiber. Efforts were made to identify their material properties and manufacturing techniques of the three types of pigments. Color difference analysis was performed by distributing the pigments in large areas to reveal characteristic differences according to the color difference of the pigment. We found that green-type pigments are malachite and atacamite, red-type pigments are hematite based on the element Fe, and white-type pigments are quartz and muscovite based on the elements Si and Al. In addition, as the size distribution of the three types of pigments is wide and has a multi-peak distribution curve, it was concluded that the pigments were used without purification.
The performance of BERT as data representation of text clustering
Authors: Subakti Alvin , Murfi Hendri , Hariadi Nora
Published: February 2022

Text clustering is the task of grouping a set of texts so that text in the same group will be more similar than those from a different group. The process of grouping text manually requires a significant amount of time and labor. Therefore, automation utilizing machine learning is necessary. One of the most frequently used method to represent textual data is Term Frequency Inverse Document Frequency (TFIDF). However, TFIDF cannot consider the position and context of a word in a sentence. Bidirectional Encoder Representation from Transformers (BERT) model can produce text representation that incorporates the position and context of a word in a sentence. This research analyzed the performance of the BERT model as data representation for text. Moreover, various feature extraction and normalization methods are also applied for the data representation of the BERT model. To examine the performances of BERT, we use four clustering algorithms, i.e., k-means clustering, eigenspace-based fuzzy c-means, deep embedded clustering, and improved deep embedded clustering. Our simulations show that BERT outperforms TFIDF method in 28 out of 36 metrics. Furthermore, different feature extraction and normalization produced varied performances. The usage of these feature extraction and normalization must be altered depending on the text clustering algorithm used.