bluuebunny/crossref_metadata_2025 huggingface dataset:
Governance challenges for Indonesian pond aquaculture: a case study of milkfish production in Gresik
Authors: Riany Citra Fitrie , Partelow Stefan , Nagel Ben
Published: September 2023

Introduction

An important blind spot in current aquaculture governance research and practice is recognizing the high dependency of the sector on commons (i.e., shared resources), and their interconnectivity with other sectors such as agriculture. This is particularly evident in Indonesia where there is general lack of social and governance research on aquaculture. The purpose of this study is to: (1) identify the commons that need to be governed in pond aquaculture systems, and (2) identify the arising governance challenges from those commons and potential solutions.

Methods

We apply Elinor Ostrom’s Socio-Ecological Systems Framework (SESF) to identify variables influencing collective action through semi-structuredinterviews with relevant key informant actors in a milkfish aquaculture village in Gresik, Indonesia. Our case study approach allows for a rich description through qualitative data to understand system interactions. We interviewed 22 stakeholders including regional and local government officials, fish farmers (owners and workers), patrons and local academics.

Results and discussion

Our findings indicate five governance challenges influencing collective action: (1) limited access to capital and dependence on patrons, (2) lack of govern institutions, (3) continued government program failure, (4) lack of community leadership, and (5) lack of motivation and incentives to collectively act. These imply that governance policies may be more successful if monitoring mechanisms are applied to ensure that government funding is more precisely targeted at improving the livelihoods of traditional fish farmers. Furthermore, attempts to support social capital, network structures, and improve trust-reciprocity among relevant stakeholders (i.e., farmers, extension officers, private business actors) in the form of capacity building is likely more effective than other monetary incentives or the enforcement of top-down rules that don’t fit local needs.
Ewing sarcoma from molecular biology to the clinic
Authors: Dupuy Maryne , Lamoureux François , Mullard Mathilde , Postec Anaïs , Regnier Laura , Baud’huin Marc , Georges Steven , Brounais-Le Royer Bénédicte , Ory Benjamin , Rédini Françoise , Verrecchia Franck
Published: September 2023

In Europe, with an incidence of 7.5 cases per million, Ewing sarcoma (ES) is the second most common primary malignant bone tumor in children, adolescents and young adults, after osteosarcoma. Since the 1980s, conventional treatment has been based on the use of neoadjuvant and adjuvant chemotherapeutic agents combined with surgical resection of the tumor when possible. These treatments have increased the patient survival rate to 70% for localized forms, which drops drastically to less than 30% when patients are resistant to chemotherapy or when pulmonary metastases are present at diagnosis. However, the lack of improvement in these survival rates over the last decades points to the urgent need for new therapies. Genetically, ES is characterized by a chromosomal translocation between a member of the FET family and a member of the ETS family. In 85% of cases, the chromosomal translocation found is (11; 22) (q24; q12), between the EWS RNA-binding protein and the FLI1 transcription factor, leading to the EWS-FLI1 fusion protein. This chimeric protein acts as an oncogenic factor playing a crucial role in the development of ES. This review provides a non-exhaustive overview of ES from a clinical and biological point of view, describing its main clinical, cellular and molecular aspects.
Diagnostic probability classification in suspected borreliosis by a novel Borrelia C6-peptide IgG1- subclass antibody test
Authors: Nyman Dag , Nordberg Marika , Nyberg Clara , Olausson Susanne , Carlströmer Berthen Nellie , Carlsson Sten-Anders
Published: September 2023

The tick-borne multisystemic infection caused by Borrelia burgdorferi sensu lato, Lyme borreliosis, or Lyme disease, occurring in temperate regions of the northern hemisphere, continues to spread geographically with the expanding tick population. Despite the rising perceived risk of infection in the population, the clinical diagnosis of Borrelia infection is not always obvious and the most important laboratory test, antibody detection, has limited accuracy in diagnosing active disease. According to international guidelines, the primary serology test, which has a high sensitivity-low specificity, should, be verified using a high specificity confirmation test to improve the specificity. However, this enhancement in specificity comes at the cost of lower sensitivity. This two-step procedure is often omitted in everyday clinical practice. An optimal primary test would be one where no secondary tests for confirmation would be necessary. In the present study, the performance of a novel assay for quantitating IgG1-subclass antibodies to Borrelia C6-peptide was compared to a commercial reference assay of total IgG and IgM antibodies to Borrelia C6-peptide in the setting of a high endemic area for borreliosis. A derivation study on a retrospective clinical material was performed to compare the performance parameters and assess the discriminatory properties of the assays, followed by a prospective validation study. The IgG1-antibody assay achieved comparable summary performance parameters to those of the reference assay. The sensitivity was almost 100% while the specificity was about 50%. In a high-endemic setting, characterized by high background seropositivity of about 50% and disease prevalence of approximately 10%, antibody tests are unable to rule-in active Borrelia infection. The rule-out assessment of the methods revealed that of 1000 patients, 7 – 54 with negative results based on the reference method could have an active Borrelia infection. Such uncertainty was not found for the index test and may help improve the risk classification of patients.
Contribution of viral and bacterial infections to senescence and immunosenescence
Authors: Reyes Antonia , Ortiz Gerardo , Duarte Luisa F. , Fernández Christian , Hernández-Armengol Rosario , Palacios Pablo A. , Prado Yolanda , Andrade Catalina A. , Rodriguez-Guilarte Linmar , Kalergis Alexis M. , Simon Felipe , Carreño Leandro J. , Riedel Claudia A. , Cáceres Mónica , González Pablo A.
Published: September 2023

Cellular senescence is a key biological process characterized by irreversible cell cycle arrest. The accumulation of senescent cells creates a pro-inflammatory environment that can negatively affect tissue functions and may promote the development of aging-related diseases. Typical biomarkers related to senescence include senescence-associated β-galactosidase activity, histone H2A.X phosphorylation at serine139 (γH2A.X), and senescence-associated heterochromatin foci (SAHF) with heterochromatin protein 1γ (HP-1γ protein) Moreover, immune cells undergoing senescence, which is known as immunosenescence, can affect innate and adaptative immune functions and may elicit detrimental effects over the host’s susceptibility to infectious diseases. Although associations between senescence and pathogens have been reported, clear links between both, and the related molecular mechanisms involved remain to be determined. Furthermore, it remains to be determined whether infections effectively induce senescence, the impact of senescence and immunosenescence over infections, or if both events coincidently share common molecular markers, such as γH2A.X and p53. Here, we review and discuss the most recent reports that describe cellular hallmarks and biomarkers related to senescence in immune and non-immune cells in the context of infections, seeking to better understand their relationships. Related literature was searched in Pubmed and Google Scholar databases with search terms related to the sections and subsections of this review.
Fabrication of deformable patient-specific AAA models by material casting techniques
Authors: Antonuccio Maria Nicole , Gasparotti Emanuele , Bardi Francesco , Monteleone Angelo , This Alexandre , Rouet Laurence , Avril Stéphane , Celi Simona
Published: September 2023

Background

Abdominal Aortic Aneurysm (AAA) is a balloon-like dilatation that can be life-threatening if not treated. Fabricating patient-specific AAA models can be beneficial for in-vitro investigations of hemodynamics, as well as for pre-surgical planning and training, testing the effectiveness of different interventions, or developing new surgical procedures. The current direct additive manufacturing techniques cannot simultaneously ensure the flexibility and transparency of models required by some applications. Therefore, casting techniques are presented to overcome these limitations and make the manufactured models suitable for in-vitro hemodynamic investigations, such as particle image velocimetry (PIV) measurements or medical imaging.

Methods

Two complex patient-specific AAA geometries were considered, and the related 3D models were fabricated through material casting. In particular, two casting approaches, i.e. lost molds and lost core casting, were investigated and tested to manufacture the deformable AAA models. The manufactured models were acquired by magnetic resonance, computed tomography (CT), ultrasound imaging, and PIV. In particular, CT scans were segmented to generate a volumetric reconstruction for each manufactured model that was compared to a reference model to assess the accuracy of the manufacturing process.

Results

Both lost molds and lost core casting techniques were successful in the manufacturing of the models. The lost molds casting allowed a high-level surface finish in the final 3D model. In this first case, the average signed distance between the manufactured model and the reference was (−0.2±0.2) mm. However, this approach was more expensive and time-consuming. On the other hand, the lost core casting was more affordable and allowed the reuse of the external molds to fabricate multiple copies of the same AAA model. In this second case, the average signed distance between the manufactured model and the reference was (0.1±0.6) mm. However, the final model’s surface finish quality was poorer compared to the model obtained by lost molds casting as the sealing of the outer molds was not as firm as the other casting technique.

Conclusions

Both lost molds and lost core casting techniques can be used for manufacturing patient-specific deformable AAA models suitable for hemodynamic investigations, including medical imaging and PIV.
Holocene sediment source analysis and paleoclimatic significance of core KZK01 from the eastern part of the Beibu Gulf
Authors: Liang Dingyong , Xu Guoqiang , Gao Fanglei , Wen Ling , Jia Liyun , Liu Li , Jiao Dongfeng , Yang Chaoyun , Wu Shuzhuang
Published: September 2023

Identifying the sources of sediments is of great significance in reconstructing Holocene paleoclimate evolution in the Beibu Gulf and in understanding the characteristics of regional responses to changes in global climate. The Holocene paleoclimatic evolutionary history of the Beibu Gulf was investigated by chronological, geochemical, and mineralogical means using the sediments of Core KZK01 from the eastern part of the Beibu Gulf. The rare earth element (REE) distribution curves, (Gd/Yb)N and (La/Yb)N discriminant diagram, and (Gd/Lu)N and ∑LREE/∑HREE discriminant diagram indicated that the detrital materials in the eastern part of the Beibu Gulf primarily originated from Hainan Island and its proximal sources, with considerable contributions from Taiwan and Pearl River materials. Source analysis of clay minerals showed that Luzon Island was the main source of smectite, followed by Hainan Island. Rivers in Taiwan were the main sources of illite in the study area, followed by the Red River. The Red River was the main contributor of chlorite, followed by the Pearl River. Kaolinite mainly originated from Hainan Island and Guangxi. Coastal currents, surface currents, and warm currents were the main drivers of material transport. Paleoclimatic variations since the Holocene in the Beibu Gulf were divided into three stages: 12–9 cal kyr BP, 9–1.3 cal kyr BP, and 1.3 cal kyr BP to the present. During different stages of climatic evolution, drought was often accompanied by cold and humidity coexisted with warmth, and cold-dry-warm-humid alternation is characterized by significant phases. The illite crystallinity clearly recorded the extreme cold events, such as Bond Events (except Bond6) and the Younger Dryas, and the change trend was essentially consistent with the regional climate record, reflecting the control of global climate change on the process of land–sea interaction in the tropical region. Furthermore, it highlights the great potential of illite crystallinity as a proxy indicator for reconstructing the surface chemical weathering processes of the region.
Relationship between PM2.5 pollution and firms’ emissions in Shaanxi Province, China
Authors: Zhao Jie , Yuan Linjiang , Jia Ce , Guan Panbo
Published: September 2023

The relationship between the high-frequency time series of PM2.5 in the atmosphere and the air pollutants emitted by industrial firms is not yet fully understood. This study aimed to identify independent PM2.5 clustering regions in Shaanxi Province and to evaluate the spatio-temporal correlations of PM2.5 concentrations and pollutant emissions from industrial firms in these regions. To accomplish this, daily data on PM2.5 concentrations and air pollutants emitted by industrial firms were analyzed using the K-means spatial clustering method and cross-wavelet transformation. The results show that: 1) PM2.5 concentrations in Shaanxi Province can be divided into three independent clustering regions. 2) The lagged impact of industrial emissions on PM2.5 concentrations were about 1/4-1/2 period. 3) PM2.5 was mainly influenced by particulate matter (PM) emissions from industrial plants during the period of 16–32 days, while nitrogen oxides (NOx) significantly affected PM2.5 concentrations during the period of 32–64 days. 4) Emissions of PM, NOx, and sulfur dioxide (SO2) more significantly affect PM2.5 concentrations in northern and central Shaanxi, and pollutants emitted by firms in the thermal power generation, utility, and steel industries had more significant effects on PM2.5 concentrations than those emitted by the cement manufacturing and electric power industries. During the COVID-19 shutdown, the emissions of firms cannot significantly affect PM2.5 concentrations. These findings suggest that emission reduction initiatives should consider industrial, regional, and periodic differences to reduce PM2.5 pollution during winter.
Effect of regional body composition changes on bone density remodeling after sleeve gastrectomy
Authors: Yang Di , Xu Rongrong , Tu Yinfang , Xiao Yunfeng , Zhang Hongwei , Liu Weijie , Zhang Pin , Yu Haoyong , Bao Yuqian , Yang Ying , Han Junfeng
Published: September 2023

Background

Sleeve gastrectomy (SG) results in bone mineral density (BMD) loss and varying body composition parameters. However, the effects of body compositions on bone health are controversial. In order to accurately demonstrate their relationship and provide new insights into the causes of BMD loss after sleeve gastrectomy, this study is aimed to investigate the role of changes in body composition in BMD loss 12 months after SG.

Methods

41 Chinese individuals with obesity (25 women and 16 men) who underwent SG were prospectively examined for at least 12 months. Measurements of anthropometrics, body composition, BMD and blood samples were collected.

Results

For 12 months, the femoral neck (FN) BMD and total hip (TH) BMD decreased significantly compared with baseline in both sexes but not lumbar spine (LS) BMD. Greater TH BMD loss was observed in men than in women. For the first 6 months post-SG, the FN BMD loss was positively associated with the estimated fat free mass index (eFFMI) reduction in women (adjusted β = 0.77, P = 0.004) and positively associated with reduction of subcutaneous fat area (SFA) in men (r = 0.931, P = 0.007). For 12 months post-SG, the FN BMD loss was negatively associated with visceral fat area (VFA) reduction in women (adjusted β = -0.58, P = 0.027) and men (adjusted β = -0.68, P = 0.032). TH BMD loss was positively associated with waist circumference reduction in women (r = 0.448, P = 0.028).

Conclusion

FN and TH BMD decrease after SG in both women and men. The changes in body compositions are associated with BMD loss at different time points and bone sites. Our data emphasize the limitation of simply taking the total weight loss (% TWL) as an influencing factor of bone mineral density and the necessity of delineating body composition in relevant studies.
Multiple uremic tumoral calcinosis in periarticular soft tissues with chronic renal failure: a case report
Authors: He Lijin , Li Meifang , Lin Chenlan , Yan Kunlong , Yang Chengmin , Tang Jing
Published: September 2023

Uremic tumoral calcinosis (UTC) is an uncommon and severe complication of hemodialysis therapy. The most important pathogenic factor involved in UTC is an increase in calcium-phosphorus products. We report here a patient undergoing hemodialysis for renal failure caused by hypertensive nephropathy who presented multiple UTCs in the right shoulder, left elbow and wrist. After surgical excision, they all recurred, with a similar UTC in the left shoulder. By observing the imaging features of various imaging examinations during the whole period of this case, including X-ray, computed tomography (CT), magnetic resonance imaging (MRI), and single-photon emission computed tomography (SPECT), we highlight the importance of imaging for evaluating the state of UTC regarding treatment options, further deepening our understanding of the imaging manifestations for this disease and their clinical significance.
Survey of epiphytic microalgae to evaluate risk of ciguatera fish poisoning across natural and artificial reefs in North Carolina
Authors: Lim Prisca , Paxton Avery B. , Taylor J. Christopher , Hall Nathan S.
Published: September 2023

Epiphytic microalgae are important contributors to the carbon and nutrient cycles yet are often overlooked during ecological surveys. In reef habitats, epiphytes are often found living on host organisms, including seaweeds or corals, and can influence community composition of higher trophic level taxa. Hence, understanding how epiphytes respond to different reef substrate materials can help inform designs of substrates intentionally deployed to form artificial reefs which can encourage high biodiversity and ecological functioning. One such epiphyte, Gambierdiscus spp., is a harmful benthic dinoflagellate which produces toxins that bioaccumulate and cause ciguatera fish poisoning (CFP) when contaminated fish is consumed by humans. CFP is one of the most frequently reported seafood-associated illnesses around the world, occurring most often near tropical reefs. We surveyed the epiphytes amongst 13 natural and artificial reef sites located off the coast of North Carolina to determine the role of the reefs’ foundational substrate (e.g., natural marl, steel or concrete) on structuring the epiphyte community with an emphasis on Gambierdiscus spp. abundance. No Gambierdiscus spp. were detected among the sampled reefs, likely due to suboptimal water temperatures. An ex-situ substrate preference experiment for Gambierdiscus spp. was performed using marl to represent natural rocky reefs, and steel and concrete to represent artificial reefs. Experimental results indicated that Gambierdiscus spp. grew fastest in the presence of marl and density decreased significantly when exposed to steel. However, steel artificial reefs had the highest average epiphyte biomass and species richness amongst the sampled reefs. 18s rRNA gene sequence analysis revealed that natural reefs were more likely to be dominated by dinoflagellates, whereas steel and concrete reefs were dominated by diatoms. We found that epiphyte composition was related to material at a phylum level, but seaweed hosts played a more significant role at the species level. These findings suggest that CFP risk is relatively low on the reefs studied but natural reefs would likely be preferentially colonized by Gambierdiscus spp. should ambient conditions become appropriate.
Abl kinases can function as suppressors of tumor progression and metastasis
Authors: Marchal Melissa A. , Moose Devon L. , Varzavand Afshin , Jordan Nicole E. , Taylor Destiney , Tanas Munir R. , Brown James A. , Henry Michael D. , Stipp Christopher S.
Published: September 2023

Introduction

Abl family kinases function as proto-oncogenes in various leukemias, and pro-tumor functions have been discovered for Abl kinases in many solid tumors as well. However, a growing body of evidence indicates that Abl kinases can function to suppress tumor cell proliferation and motility and tumor growth in vivo in some settings.

Methods

To investigate the role of Abl kinases in tumor progression, we used RNAi to generate Abl-deficient cells in a model of androgen receptor-indifferent, metastatic prostate cancer. The effect of Abl kinase depletion on tumor progression and metastasis was studied in an in vivo orthotopic model, and tumor cell motility, 3D growth, and signaling was studied in vitro.

Results

Reduced Abl family kinase expression resulted in a highly aggressive, metastatic phenotype in vivo that was associated with AKT pathway activation, increased growth on 3D collagen matrix, and enhanced cell motility in vitro. Inhibiting AKT pathway signaling abolished the increased 3D growth of Abl-deficient cells, while treatment with the Abl kinase inhibitor, imatinib, promoted 3D growth of multiple additional tumor cell types. Moreover, Abl kinase inhibition also promoted soft-agar colony formation by pre-malignant fibroblasts.

Conclusions

Collectively, our data reveal that Abl family kinases can function to suppress malignant cell phenotypes in vitro, and tumor progression and metastasis in vivo.
Almonertinib plus chemotherapy versus almonertinib alone in second-line treatment of advanced non-small cell lung cancer with mutated epidermal growth factor receptor: a retrospective study
Authors: Fang Xiaoxu , Xiang Yan , Lu Kaihua
Published: September 2023

Objective

This study mainly observes the efficacy and safety of almonertinib plus chemotherapy compared with almonertinib alone in the second-line treatment of advanced non-small cell lung cancer (NSCLC) with mutated epidermal growth factor receptor (EGFR).

Methods

In this study, clinical data of 68 patients with advanced NSCLC who were treated in Jiangsu Provincial People’s Hospital and Nanjing Chest Hospital between April 2020 and December 2022 were collected. Among them, the study group (n=30) received second-line almonertinib combined with platinum-based chemotherapy, while the control group (n=38) received almonertinib alone. The near-term and long-term effects and adverse events of the two groups were compared respectively.

Results

The median follow-up time until 31 December 2022 was 16.3 months (95% CI: 11.32-21.34). Results of chi-square analysis showed no statistically significant difference in objective response rate (ORR) and disease control rate (DCR) between the study group and the control group (56.73% vs. 55.3%, P>0.05; 100% vs. 86.8%, P>0.05). Log-rank test comparing the two groups revealed that the median progression-free survival (mPFS) of the study group was significantly longer than that of the control group by 3.1 months (12.7 vs. 9.6 months, P=0.01). Multivariate COX proportional risk model showed a statistically significant effect of treatment method and PS score on PFS (HR=0.43, P=0.023; HR=3.82, P=0.001). In terms of safety, most of the adverse events (AEs) were mild, with no grade 4-5 in the two groups, and the overall tolerance of patients was good.

Conclusion

For advanced NSCLC patients with EGFR mutations, second-line treatment with almonertinib plus chemotherapy significantly improved PFS compared with almonertinib alone without a significant increase in adverse events, providing efficacy and safety.
Evaluation of single-fraction high dose FLASH radiotherapy in a cohort of canine oral cancer patients
Authors: Børresen Betina , Arendt Maja L. , Konradsson Elise , Bastholm Jensen Kristine , Bäck Sven ÅJ. , Munck af Rosenschöld Per , Ceberg Crister , Petersson Kristoffer
Published: September 2023

Background

FLASH radiotherapy (RT) is a novel method for delivering ionizing radiation, which has been shown in preclinical studies to have a normal tissue sparing effect and to maintain anticancer efficacy as compared to conventional RT. Treatment of head and neck tumors with conventional RT is commonly associated with severe toxicity, hence the normal tissue sparing effect of FLASH RT potentially makes it especially advantageous for treating oral tumors. In this work, the objective was to study the adverse effects of dogs with spontaneous oral tumors treated with FLASH RT.

Methods

Privately-owned dogs with macroscopic malignant tumors of the oral cavity were treated with a single fraction of ≥30Gy electron FLASH RT and subsequently followed for 12 months. A modified conventional linear accelerator was used to deliver the FLASH RT.

Results

Eleven dogs were enrolled in this prospective study. High grade adverse effects were common, especially if bone was included in the treatment field. Four out of six dogs, who had bone in their treatment field and lived at least 5 months after RT, developed osteoradionecrosis at 3-12 months post treatment. The treatment was overall effective with 8/11 complete clinical responses and 3/11 partial responses.

Conclusion

This study shows that single-fraction high dose FLASH RT was generally effective in this mixed group of malignant oral tumors, but the risk of osteoradionecrosis is a serious clinical concern. It is possible that the risk of osteonecrosis can be mitigated through fractionation and improved dose conformity, which needs to be addressed before moving forward with clinical trials in human cancer patients.
Association of dietary fatty acid intake with hypertension in children and adolescents: evidence from the NHANES 2005–2018
Authors: Li Xiumin , Qi Fengqin , Zhao Zhihong , Ma Jinbang
Published: September 2023

Aim

This study aims to evaluate the association between dietary fatty acid intake and hypertension in children and adolescents.

Methods

This cross-sectional study used data of children and adolescents aged 8–17 years from the National Health and Nutrition Examination Survey (NHANES) 2005–2018. Dietary intake of total fat and fatty acid was evaluated via two 24-h dietary recall interviews. Multivariate logistic regression models were used to assess the association between fatty acid intake and hypertension, with odds ratios (ORs) and 95% confidence intervals (CIs) calculated. A subgroup analysis was conducted according to gender, age, and body mass index Z-score.

Results

This study included 13,330 subjects, of which 11,614 were non-hypertensive and 1,716 were hypertensive. Higher intake of total polyunsaturated fatty acids (PUFAs) was associated with significantly lower odds of hypertension (OR = 0.85, 95% CI: 0.74–0.97, P = 0.018). No significant associations were found between the density of total saturated fatty acid, monounsaturated fatty acids, and PUFAs and the odds of hypertension (all P > 0.05). Increased intake of omega-3 (OR = 0.82, 95% CI: 0.72–0.93, P = 0.002) and omega-6 (OR = 0.86, 95% CI: 0.75–0.98, P = 0.025) PUFAs, octadecatrienoic acid (OR = 0.82, 95% CI: 0.72–0.93, P = 0.003), and octadecadienoic acid (OR = 0.86, 95% CI: 0.75–0.98, P = 0.025) was associated with significantly lower odds of hypertension, and individuals with higher omega-6/omega-3 ratio had significantly higher odds of hypertension (OR = 1.09, 95% CI: 1.02–1.17, P = 0.025). The density of omega-3 PUFAs (OR = 0.86, 95% CI: 0.78–0.95, P = 0.004) and octadecatrienoic acid (OR = 0.87, 95% CI: 0.78–0.96, P = 0.006) was inversely associated with the odds of hypertension, and the omega-6/omega-3 ratio was positively associated with the odds of hypertension (OR = 1.09, 95% CI: 1.02–1.17, P = 0.012).

Conclusion

Total PUFA intake was negatively associated with the odds of hypertension in children and adolescents. Higher intake of omega-3 and omega-6 PUFAs, octadecatrienoic acid, and octadecadienoic acid, as well as density of omega-3 PUFAs and octadecatrienoic acid, was associated with lower odds of hypertension.
A hypothermia mimetic molecule (zr17-2) reduces ganglion cell death, gliosis, and electroretinogram distortion in male rats subjected to perinatal asphyxia
Authors: Rey-Funes Manuel , Fernández Juan Carlos , Peláez Rafael , Soliño Manuel , Contartese Daniela S. , Ciranna Nicolás S. , Nakamura Ronan , Sarotto Aníbal , Dorfman Verónica B. , Zapico José M. , Ramos Ana , de Pascual-Teresa Beatriz , López-Costa Juan José , Larrayoz Ignacio M. , Martínez Alfredo , Loidl César Fabián
Published: September 2023

Introduction: Perinatal asphyxia (PA) represents a major problem in perinatology and may cause visual losses, including blindness. We, and others, have shown that hypothermia prevents retinal symptoms associated to PA. In the present work, we evaluate whether a hypothermia mimetic small molecule, zr17-2, has similar effects in the context of PA.

Methods: Four experimental groups were studied in male rats: Naturally born rats as controls (CTL), naturally born rats injected s.c. with 50 µL of 330 nmols/L zr17-2 (ZR), animals that were exposed to PA for 20 min at 37°C (PA), and rats that were exposed to PA and injected with zr17-2 (PA-ZR). Forty-five days after treatment, animals were subjected to electroretinography. In addition, morphological techniques (TUNEL, H&E, multiple immunofluorescence) were applied to the retinas.

Results: A reduction in the amplitude of the a- and b-wave and oscillatory potentials (OP) of the electroretinogram (ERG) was detected in PA animals. Treatment with zr17-2 resulted in a significant amelioration of these parameters (p < 0.01). In PA animals, a large number of apoptotic cells was found in the GCL. This number was significantly reduced by treatment with the small molecule (p < 0.0001). In a similar way, the thickness of the inner retina and the intensity of GFAP immunoreactivity (gliosis) increased in PA retinas (p < 0.0001). These parameters were corrected by the administration of zr17-2 (p < 0.0001). Furthermore, injection of the small molecule in the absence of PA did not modify the ERG nor the morphological parameters studied, suggesting a lack of toxicity.

Discussion: In conclusion, our results indicate that a single s.c. injection of zr17-2 in asphyctic neonates may provide a novel and efficacious method to prevent the visual sequelae of PA.
The pathology of oxidative stress-induced autophagy in a chronic rotator cuff enthesis tear
Authors: Prasetia Renaldi , Purwana Siti Zainab Bani , Lesmana Ronny , Herman Herry , Chernchujit Bancha , Rasyid Hermawan Nagar
Published: September 2023

Partial-thickness rotator cuff tears (PTRCTs) are often found in daily orthopedic practice, with most of the tears occurring in middle-aged patients. An anaerobic process and imbalanced oxygenation have been observed in PTRCTs, resulting in oxidative stress. Studies have shown the roles of oxidative stress in autophagy and the potential of unregulated mechanisms causing disturbance in soft tissue healing. This article aims to review literature works and summarize the potential pathology of oxidative stress and unregulated autophagy in the rotator cuff enthesis correlated with chronicity. We collected and reviewed the literature using appropriate keywords, in addition to the manually retrieved literature. Autophagy is a normal mechanism of tissue repair or conversion to energy needed for the repair of rotator cuff tears. However, excessive mechanisms will degenerate the tendon, resulting in an abnormal state. Chronic overloading of the enthesis in PTRCTs and the hypovascular nature of the proximal tendon insertion will lead to hypoxia. The hypoxia state results in oxidative stress. An autophagy mechanism is induced in hypoxia via hypoxia-inducible factors (HIFs) 1/Bcl-2 adenovirus E1B 19-kDa interacting protein (BNIP) 3, releasing beclin-1, which results in autophagy induction. Reactive oxygen species (ROS) accumulation would induce autophagy as the regulator of cell oxidation. Oxidative stress will also remove the mammalian target of rapamycin (mTOR) from the induction complex, causing phosphorylation and initiating autophagy. Hypoxia and endoplasmic reticulum (ER) stress would initiate unfolded protein response (UPR) through protein kinase RNA-like ER kinase (PERK) and activate transcription factor 4, which induces autophagy. Oxidative stress occurring in the hypovascularized chronic rotator cuff tear due to hypoxia and ROS accumulation would result in unregulated autophagy directly or autophagy mediated by HIF-1, mTOR, and UPR. These mechanisms would disrupt enthesis healing.
Neural substrates underlying rhythmic coupling of female reproductive and thermoregulatory circuits
Authors: Grant Azure D. , Kriegsfeld Lance J.
Published: September 2023

Coordinated fluctuations in female reproductive physiology and thermoregulatory output have been reported for over a century. These changes occur rhythmically at the hourly (ultradian), daily (circadian), and multi-day (ovulatory) timescales, are critical for reproductive function, and have led to the use of temperature patterns as a proxy for female reproductive state. The mechanisms underlying coupling between reproductive and thermoregulatory systems are not fully established, hindering the expansion of inferences that body temperature can provide about female reproductive status. At present, numerous digital tools rely on temperature to infer the timing of ovulation and additional applications (e.g., monitoring ovulatory irregularities and progression of puberty, pregnancy, and menopause are developed based on the assumption that reproductive-thermoregulatory coupling occurs across timescales and life stages. However, without clear understanding of the mechanisms and degree of coupling among the neural substrates regulating temperature and the reproductive axis, whether such approaches will bear fruit in particular domains is uncertain. In this overview, we present evidence supporting broad coupling among the central circuits governing reproduction, thermoregulation, and broader systemic physiology, focusing on timing at ultradian frequencies. Future work characterizing the dynamics of reproductive-thermoregulatory coupling across the lifespan, and of conditions that may decouple these circuits (e.g., circadian disruption, metabolic disease) and compromise female reproductive health, will aid in the development of strategies for early detection of reproductive irregularities and monitoring the efficacy of fertility treatments.
Mummy berry pseudosclerotia survive for several years
Authors: Pscheidt J. W. , Bassinette J. P. , Warneke B.
Published: September 2023

Organic blueberry production in the PNW has many challenges, including diseases like mummy berry caused by the fungus Monilinia vaccinii-corymbosi (Reade) Honey. Management recommendations focus on reducing overwintering pseudosclerotia, however, it is unknown how long they survive. Based on qualitative observations pseudosclerotia are hypothesized to survive multiple years after contact with the soil surface. The development of apothecia from M. vaccinii-corymbosi pseudosclerotia was evaluated over multiple years at a location without a history of blueberry production. A total of 1,000 pseudosclerotia were placed on field soil plots in 2018 and replicated eight times. Another 100 pseudosclerotia were placed in wire corrals on field soil and replicated fifteen times. Plots and corrals were regularly examined each spring for the emergence of apothecia. The pseudosclerotia were able to survive, germinate, and produce apothecia for up to five years after their placement. Very few pseudosclerotia produced apothecia in any year, varying from 0 to 18 at any observed time. Pieces of partial or whole pseudosclerotia were observed for up to three years after placement. Our study shows that a pseudosclerotial “seed bank” exists under blueberry bushes, necessitating a long-term implementation of mummy berry cultural management tactics.
Culturally sensitive active ageing seen through the lens of the welfare theory of health: assistant nurses’ views
Authors: Johansson Carl , Gustafsson Lena-Karin , Lindberg Daniel , Asztalos Morell Ildikó
Published: September 2023

Assistant nurses caring for older adults with immigrant backgrounds are on the front lines of a practical, theoretical, and policy battlefield. They need to implement culturally sensitive care provision while not overstating the importance of culture, thereby, contributing to a negative picture of older immigrants as especially problematic. One proposed way to strike such a balance is the welfare theory of health (WTH). In this article, we let assistant nurses apply the WTH to a series of questions in four different vignettes representing the life stories of older persons who characterize typical dilemmas described by the theory. The results show that, through the lens of the WTH, assistant nurses looked for individual care preferences rather than stereotypical ideas about cultural characteristics. Further, the assistant nurses expressed a desire to get to know the persons more deeply to better interpret and understand their individual preferences. Thus, the theoretical framework is useful not only for exposing vulnerabilities to which some older adults with immigrant backgrounds may be exposed, but also for finding ways to mitigate the vulnerability by illuminating vital life goals and using them as a framework to organize care. This approach allows for mitigating the gap between the vital life goals and available resources to achieve a holistic state of health.
Sequential mediation of early temperament and eating behaviors in the pathways from feeding practices to childhood overweight and obesity
Authors: Zhang Xiaoning , Zhou Qiong , Vivor Nathaniel Kossi , Liu Wei , Cao Junli , Wang Sheng
Published: September 2023

Introduction

Childhood eating behaviors and temperament may have important implication for constructing the pathways from maternal feeding practices to childhood overweight and obesity (OW/OB). Examining multiple feeding styles simultaneously to childhood OW/OB is critical through the mediators of early childhood temperament and eating behaviors.

Methods

This cross-sectional study recruited mothers mainly responsible for child care from two hospitals and two healthcare centers in eastern China. Sociodemographic characteristics, and data from the Infant Feeding Style Questionnaire (IFSQ), the short form of Children Behavior Questionnaire [Revised (IBQ-RSF)], and the Child Eating Behavior Questionnaire for toddler (CEBQ-T) were collected. Weight and recumbent length were measured to calculate the age- and sex-specific body mass index (BMI) z-scores (BMIz). The structural equation modeling (SEM) approach was used to examine direct and indirect pathways from five maternal feeding styles to childhood OW/OB through temperament and eating behaviors.

Results

A total of 486 children were recruited, 73 (15.02%) children were OW/OB; the age of the children was 14.55 (SD = 5.14) months, and the age of the mothers was 29.90 (SD = 3.63) years. The responsive feeding exerted significant direct (β = −0.098), indirect (β = −0.136) and total (β = −0.234) effects on childhood OW/OB. Restrictive feeding had significant direct (β = 0.222), indirect (β = 0.102) and total (β = 0.324) effects on childhood OW/OB. Indulgent feeding had significant direct (β = 0.220), indirect (β = 0.063), and total (β = 0.283) effects on childhood OW/OB. Pressuring feeding had significant direct (β = −0.116), indirect (β = −0.096) and total (β = −0.212) effects on childhood OW/OB.

Discussion

There was a direct effect of feeding practices on childhood OW/OB; feeding practices indirectly predicted childhood OW/OB through temperament and eating behaviors in children aged 6–23 months. This study could help governments agencies, policymakers, and healthcare workers to establish optimal intervention programs targeting feeding practices through childhood eating behaviors and temperament to prevent childhood OW/OB.
The safety and efficacy of laparoscopic retrograde appendicectomy, base-to-tip approach
Authors: Ko Ara , Lindsay Perry , Choi Julian
Published: September 2023

Background

Laparoscopic appendicectomy is one of the most frequently performed surgical procedures worldwide. There is limited evidence evaluating the role and safety of laparoscopic retrograde appendicectomy (LRA), base to tip approach, compared to standard laparoscopic antegrade appendicectomy (LAA), tip to base approach. This study aims to assess the safety of LRA compared to LAA in terms of intra-abdominal collection (IAC) rate and using Sunshine Appendicitis Grading System (SAGS).

Methods

Records of two-hundred and seventy-three patients undergoing laparoscopic appendicectomy by LAA and LRA approaches were analysed. The severity of appendicitis was rated using a standardised Sunshine Appendicitis Grading System (SAGS) score intra-operatively. The primary outcome measure was the occurrence of an intra-abdominal collection, and secondary measures were procedure time, post-operative length of stay and other complications.

Results

Of the two-hundred and seventy-three patients, there were two patients who developed an intra-abdominal collection. Both patients were in the LAA group with SAGS IV scores. Between SAGS IV patients, Chi-squared p value of 0.6691. Therefore, there was no statically significant difference in the intra-abdominal collection (IAC) rate between LAA and LRA groups from this study.

Conclusions

The current study has shown that laparoscopic retrograde appendicectomy (LRA) does not increase risk of intra-abdominal collection compared to laparoscopic antegrade appendicectomy (LAA) within the limit of this study.
Comparative Study of IGBT and SiC MOSFET Three-Phase Inverter: Impact of Parasitic Capacitance on the Output Voltage Distortion
Authors: Poolphaka Paisak , Jamshidpour Ehsan , Lubin Thierry , Baghli Lotfi , Takorabet Noureddine
Published: September 2023

This study investigates the nonlinearities in three-phase inverters for SiC-based systems and compares their performance to IGBT-based systems. An analytical model of inverter voltage distortion is developed, which accounts not only for dead time (td), switching delay time, switching frequency (fs), and voltage drops of power devices, but also for output parasitic capacitance (Cout). Experimental tests validate the model, which provides a more accurate estimate of the inverter’s output phase voltage distortion. The power device characteristics are obtained from datasheets, while Cout is determined through experimentation. Three-phase inverters with varying switching frequencies, fundamental frequencies, and dead-time values are used in simulations and experiments to determine the influence of nonlinearity on phase voltage deviation and current distortion. The results show that, due to SiC devices’ faster switching time, the phase voltage deviation and phase current distortion are lower in SiC-based inverters than in IGBT-based ones for high-frequency applications, as the dead time can be reduced.
Late Adolescents’ Texting Experiences with Family: Mixed-Method Analysis for Understanding Themes and Sentiments
Authors: Sun Xiaoran , Dworkin Jodi , LeBouef Samantha
Published: September 2023

(1) Background: Texting is a prevalent communication method between late adolescents and their families that has implications for their relationships and well-being. This study used mixed-method analysis to understand what late adolescents, specifically college students, text their families about (i.e., themes), and how they feel about their family texting experiences (i.e., sentiments). (2) Methods: Our analyses used text transcripts of semi-structured individual interviews from 19 college students (aged 18–22 years, 13 female, 10 students of color), with 357 sentences/passages coded in total. (3) Results: Inductive content analysis revealed four themes: emotional support, relationship maintenance, conflict, and difficult conversations. Quantitative sentiment analysis revealed the highest level of positive sentiment attached to emotional support, and the highest level of negative sentiment attached to difficult conversations. The interviews that covered more conflict-based themes tended to have higher positive and negative sentiments. Differences between participants texting with their mothers and fathers were also revealed. (4) Conclusions: This research advances the understanding, in terms of both content and emotions, of the texting interactions with family members among late adolescents, along with providing methodological contributions, by highlighting the utility of the mixed-method analysis of interview data.
Stiffness Design of Active Capture Claw-Type Docking Mechanism for Lunar Sample Return
Authors: Wang Weijun , Zhang Chongfeng , Qi Chenkun , Fu Lijia , Wang Shigang
Published: September 2023

The docking mechanism is the key system for realizing the lunar-orbit docking mission of two spacecraft, which needs to have both capture correction and connection hold functions. The different stiffness requirements between the capture correction process, where low stiffness is desired, and the connection hold process, where high stiffness is desired, pose a significant challenge to the design of the docking mechanism. In this paper, an active capture claw docking mechanism is designed. Under the constraints of being lightweight and having an envelope size, three sets of independent claw mechanisms are designed using the modular design idea to achieve the performance optimization and function integration of the docking mechanisms. The theoretical model of the collision dynamics between the active and passive docking mechanisms is established; the stiffness value range of the docking mechanism is determined, and the typical docking conditions are simulated and verified. The results show that the stiffness design in this paper can satisfy the requirements of the two docking processes. The active capture claw docking mechanism developed was applied to the lunar surface sample return mission successfully and played an important role in the lunar-orbit docking mission.
A Smart Farm DNN Survival Model Considering Tomato Farm Effect
Authors: Kim Jihun , Ha Il Do , Kwon Sookhee , Jang Ikhoon , Na Myung Hwan
Published: September 2023

Recently, smart farming research based on artificial intelligence (AI) has been widely applied in the field of agriculture to improve crop cultivation and management. Predicting the harvest time (time-to-harvest) of crops is important in smart farming to solve problems such as planning the production schedule of crops and optimizing the yield and quality. This helps farmers plan their labor and resources more efficiently. In this paper, our concern is to predict the time-to-harvest (i.e., survival time) of tomatoes on a smart farm. For this, it is first necessary to develop a deep learning modeling approach that takes into account the farm effect on the tomato plants, as each farm has multiple tomato plant subjects and outcomes on the same farm can be correlated. In this paper, we propose deep neural network (DNN) survival models to account for the farm effect as a fixed effect using one-hot encoding. The tomato data used in our study were collected on a weekly basis using the Internet of Things (IoT). We compare the predictive performance of our proposed method with that of existing DNN and statistical survival modeling methods. The results show that our proposed DNN method outperforms the existing methods in terms of the root mean squared error (RMSE), concordance index (C-index), and Brier score.