bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure S6 from The Novel ATR Inhibitor Tuvusertib (M1774) Induces Replication Protein Overexpression and Broad Synergy with DNA-targeted Anticancer Drugs
Authors: Jo Ukhyun , Arakawa Yasuhiro , Zimmermann Astrid , Taniyama Daiki , Mizunuma Makito , Jenkins Lisa M. , Maity Tapan K. , Kumar Suresh , Zenke Frank T. , Takebe Naoko , Pommier Yves
Published: July 2024

This figure shows representative images in the treatment of TPT (topotecan) and M1774 in small cell lung cancer organoids, and profiling of cell viability and DNA-damage molecules after drug treatment.
Supplementary Figure S5 from The Novel ATR Inhibitor Tuvusertib (M1774) Induces Replication Protein Overexpression and Broad Synergy with DNA-targeted Anticancer Drugs
Authors: Jo Ukhyun , Arakawa Yasuhiro , Zimmermann Astrid , Taniyama Daiki , Mizunuma Makito , Jenkins Lisa M. , Maity Tapan K. , Kumar Suresh , Zenke Frank T. , Takebe Naoko , Pommier Yves
Published: July 2024

This figure shows profiling of cell viability after M1774 treatment in DMS114 and DU145 cells, and a correlation plot between topotecan and SLFN11 expression.
Table S3 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Table S3 shows PrP and PP-based BKIDC chemical structures.
Table S1 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Table S1 shows BKIDC-1553 intratumoral concentrations after at least 4 weeks of thrice weekly oral dosing at 20 mg/kg.
Supplementary Figure S1 from The Novel ATR Inhibitor Tuvusertib (M1774) Induces Replication Protein Overexpression and Broad Synergy with DNA-targeted Anticancer Drugs
Authors: Jo Ukhyun , Arakawa Yasuhiro , Zimmermann Astrid , Taniyama Daiki , Mizunuma Makito , Jenkins Lisa M. , Maity Tapan K. , Kumar Suresh , Zenke Frank T. , Takebe Naoko , Pommier Yves
Published: July 2024

This figure shows Chemical structures of clinically developing ATR inhibitors.
Figure S9 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Figure S9 shows cooperation of BKIDCs with oligomycin to induce apoptotic cell death.
Figure S7 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Figure S7 shows Western blot analysis confirming the findings with RPPA.
Figure S1 from A Compound That Inhibits Glycolysis in Prostate Cancer Controls Growth of Advanced Prostate Cancer
Authors: Uo Takuma , Ojo Kayode K. , Sprenger Cynthia C.T. , Epilepsia Kathryn S. , Perera B. Gayani K. , Damodarasamy Mamatha , Sun Shihua , Kim Soojin , Hogan Hannah H. , Hulverson Matthew A. , Choi Ryan , Whitman Grant R. , Barrett Lynn K. , Michaels Samantha A. , Xu Linda H. , Sun Vicky L. , Arnold Samuel L.M. , Pang Haley J. , Nguyen Matthew M. , Vigil Anna-Lena B.G. , Kamat Varun , Sullivan Lucas B. , Sweet Ian R. , Vidadala Ram , Maly Dustin J. , Van Voorhis Wesley C. , Plymate Stephen R.
Published: July 2024

Figure S1 shows evaluation of Hexokinase activity using LNCaP crude extracts and recombinant enzyme.
Figure 4 from Zinc-alpha-2-glycoprotein Secreted by Triple-Negative Breast Cancer Promotes Peritumoral Fibrosis
Authors: Verma Surbhi , Giagnocavo Stephanie D. , Curtin Meghan C. , Arumugam Menusha , Osburn-Staker Sandra M. , Wang Guoying , Atkinson Aaron , Nix David A. , Lum David H. , Cox James E. , Hilgendorf Keren I.
Published: July 2024

ZAG is important for MDA-MB-468 tumorigenesis. A and B, Depletion of ZAG inhibits xenograft growth of MDA-MB-468 cells as determined by (A) tumor volume measurements over time or (B) weight of excised tumor at the end point. C, MDA-MB-468 cells depleted for ZAG show a trend of decreased proliferation in vivo. A–C, Data are mean ± SEM. Data points and n are independent mice. P values calculated using (A) two-way ANOVA followed or (B and C) Student t test. (**, P < 0.01; ****, P < 0.0001.)
Figure 2 from Zinc-alpha-2-glycoprotein Secreted by Triple-Negative Breast Cancer Promotes Peritumoral Fibrosis
Authors: Verma Surbhi , Giagnocavo Stephanie D. , Curtin Meghan C. , Arumugam Menusha , Osburn-Staker Sandra M. , Wang Guoying , Atkinson Aaron , Nix David A. , Lum David H. , Cox James E. , Hilgendorf Keren I.
Published: July 2024

The glycoprotein ZAG inhibits adipogenesis. A, Inhibition of 3T3-L1 adipogenesis is rescued with heat inactivation of the secretomes of MDA-MB-468 and MDA-MB-231 cells. B, The antiadipogenic factor of the MDA-MB-468 and MDA-MB-231 secretomes (total) is retained in the supernatant (top) fraction following centrifugation using a spin column with a molecular weight cut-off of 100,000 Da. C, Candidate antiadipogenic factors identified in the secretomes of MDA-MB-468 and MDA-MB-231 cells. D, The secretome of MDA-MB-468 cells depleted of ZAG does not inhibit 3T3-L1 adipogenesis. E, Supplementing 3T3-L1 cells with recombinant ZAG during the first 2 days of adipogenesis is sufficient to inhibit adipogenesis. A–E, All data are mean ± SD. Data points show independent biological replicates. P values calculated using one-way ANOVA followed by (A and B) Tukey’s multiple comparison test or (D) Dunnett’s multiple comparison test, or (E) two-way ANOVA followed by Šidák’s multiple comparison test. (ns, non-significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001.)
Clinical Risk Factors of Post Operative Complications in Hypospadias Repair
Authors: Fawzi Akmal , Satyagraha Paksi , Daryanto Besut
Published: July 2024

Objectives. Failed hypospadias refers to any hypospadias repair that leads to complications or causes patient dissatisfaction. Many factors influence the occurrence of complications, therefore this study aims to determine what risk factors can cause complications after hypospadias surgery.Methods. The study was conducted retrospectively by collecting 78 data of hypospadias patients who underwent surgery in Saiful Anwar General Hospital medical record from January 2015 until June 2019. age at the time of surgery, location of the external urethral meatus, degree of chordee, previous surgery history, type of urinary diversion, presence of Urinary Tract Infection (UTI), how many stages of surgery were performed, the surgical technique used at each stage and the complication recorded and analyzed.Results. Hypospadias patients who underwent surgery were 78 patients. The mean patient age was 9.96 years SD ± 6.959. Most of the ages were 11-18 years old with 28 patients. The location of the external urethral meatus was mostly found proximal to the penis of 40 patients. 44 patients were accompanied by mild chordee. 10 patients had previously undergone hypospadias surgery. 16 patients suffered from significant urinary tract infections but had no symptoms. 60 patients were inserted urethral catheter placement after hypospadias surgery. The most surgical technique used in stage 1 hypospadias surgery is Tubularized Incised Plate (TIP) Urethroplasty. While the technique used in 2 stage hypospadias surgery was Dorsal onlay preputial graft followed by TIP Urethroplasty. Complications that occurred in 1 stage hypospadias surgery were urethrocutan fistule in 9 patients and chordee in 1 patient. In 2 stage hypospadias surgery 3 patients obtained urethrocutan fistules, 2 patients with scrotal abscess and 1 patient obtained graft shrinkage. Age at surgery, location of Meatus Urethrae Externus (MUE), presence of UTI and also the choice of urinary diversion type correlated with the presence of complications. (P=0,004, P=0,033, P=0,049, P=0,000).Conclusions. Our results suggest that the success of hypospadias surgery is associated with age at surgery, location of MUE, presence of UTI and also the choice of postoperative urinary diversion type.
Case Report: Staghorn Stone Successfully Treated With Open Nephrolithotomy
Authors: Nababan Ezra Harun Florentino , Siadary Ephram Sanders Alfian , Telussa Arley Sandra
Published: July 2024

Objectives. Staghorn stones are large branching stones that fill part of all of the renal pelvis and renal calyces and they can be complete or partial depending on the level of occupancy of the collecting system. The case of this Staghorn Kidney Stone stone was reported at Leona hospital, Kupang, East Nusa Tenggara, Indonesia.Case. The patient is a 22 year old male with Staghorn renal stone with the size measured 14 x 11 cm. The patient came to the ER with complaints of low back pain and patient complains of difficulty urinating first. nephrolithotomy surgical procedure was performed to release the stone.Conclusion. The cause of the Renal staghorn stone should be considered prior to treatment as eliminating the cause and the underlying factor will reduce the complications and recurrence rates.
Varicocelectomy For Infertile Men with Clinical Varicocele and Normal Semen Parameters, In Relation to Pregnancy Rate and Recurrent Spontaneous Miscarriage Incidence: A Systematic Review
Authors: Mahaputra I Komang Bintang Satria , Indradiputra I Made Udiyana , Sanjaya I Dewa Gede Reza
Published: July 2024

Objectives. Current guidelines suggest performing varicocelectomy only for men with clinical varicocele, infertile, abnormal semen parameters, and impaired testicular development. Varicocelectomy in infertile men with normal semen parameters was deemed to not benefit fertility significantly. The purpose of this systematic review is to discuss articles that studied varicocelectomy in men with clinical varicocele, normal semen analysis, and normal women who experience infertility or spontaneous miscarriage and find out whether there is an improvement in pregnancy and live birth rate.Methods. The databases used for searching articles in this study are PubMed and Cochrane using the keywords “varicocele”, “normal semen parameters”, “recurrent miscarriage”, “sperm DNA fragmentation”, and “infertility”. The outcome of the articles sought is pregnancy rates and miscarriage rates after varicocelectomy.Result. After carrying out the article selection process, there are 2 articles used. One study is a randomized control trial and another one is a non-randomized control trial. All enrolled patients had varicocele and normal semen analysis results according to WHO guidelines 2022. The average pregnancy rate after varicocelectomy at a 12-month follow-up interval was 51% in the varicocelectomy group vs 25% in the control group. The average miscarriage rate was 6% in the varicocelectomy group vs 34% in the control group. One study showed a correlation between decreasing Sperm DNA fragmentation and pregnancy rate.Conclusion. Varicocelectomy may provide benefits in infertile couples and cases of recurrent spontaneous miscarriage with normal female factors and men with varicocele with normal semen parameters.
The Prevalence of LUTS in Men Aged ≥40 years based on the IPSS-Ina Questionnaire
Authors: Lasandara Wangga P. , Taufiqurrachman Muhammad , Handoyo Dimas Bintoro Kresna Yustisia
Published: July 2024

Objectives. LUTS (Lower Urinary Tract Symptoms) are several symptoms experienced by a person due to various pathologies that occur in the lower urinary tract. The prevalence of LUTS in ≥ 40-year-old men is high and many methods including IPSS have been made to measure LUTS. Unfortunately, the high prevalence is not followed by the number of patients who are seeking treatment for their LUTS.Methods. This observational cross-sectional study used 45 men aged ≥40 years who came to Tongauna Health Center. Each respondent was asked to fill out the IPSS questionnaire. Moreover, when the respondent has LUTS, he is also asked if he is ever looking for health care providers, where he consultations, and what the reason is if he is never looking for it.Result. Of 45 men, there are 37 men who have LUTS and only 8 asymptomatic men. Mild LUTS is the most common symptom with 22 respondents, followed by moderate with 11 respondents, and only 4 respondents who suffered from severe LUTS. Pleased (1) is the most respondent’s feel for their Quality of Life score. Only 7 respondents are ever looking for treatment, and the common reasons for them not getting treatment are their misperception about LUTS, economic factors, and attitude toward their illness.Conclusion. The number of LUTS patients≥40 years old male at Tongauna District is high, but their initiative to treat this symptom is still low due to many factors like knowledge, economic, and patient attitude.
Benign prostate obstruction in the primary care setting: A Case Report and Review
Authors: Liem Roy Andrew Halim , Putra Aulia Rahman
Published: July 2024

Introduction. Benign prostatic hyperplasia is a prevalent issue among the elderly. The prevalence continues to rise every year and disrupts the patient's quality of life. It is often observed that individuals with this disease frequently exhibit lower urinary tract symptoms (LUTS) due to bladder outlet obstruction (BOO). The case was reported at Tanjung Kasuari Community Health Centre, Sorong City, West Papua, Indonesia.Case. An elderly male, 69 years old, came with complaints of highly frequent night urination (nocturia), experienced a weak urine flow, and difficulty postponing urination (urgency). The patient was treated with empirical therapy of Oral Tamsulosin 0.4mg once daily for 2 weeks and lifestyle modifications.Conclusion. Primary care physicians play an important role in early detection, predominantly in remote locations, but in complex cases when a more precise diagnosis and further management is necessary, consider referring immediately to urologists.
Detetive climático : caderno de exercícios - Vol. 1
Authors:   Universidade de São Paulo. Escola de Artes, Ciências e Humanidades, Cseh Amanda , Cavaco Isabela Carmo , Gomes Aline da Conceição ,   Universidade de São Paulo. Instituto de Energia e Ambiente,   Universidade de São Paulo. Escola de Artes, Ciências e Humanidades
Published: July 2024

A obra "Detetive climático : caderno de exercícios - Vol. 1" integra a Série NOSS Educação para Justiça Climática, um resultado do “Projeto de Pesquisa Participativa em Ação: Educação para Justiça Climática no Vale do Ribeira” desenvolvido pelo NOSS EACH USP, com fomento internacional Climate-U, sob coordenação do INCLINE USP. O objetivo central é contribuir para que a escola se transforme em um epicentro estratégico na promoção da justiça climática, criando subsídios e inspirando educadores e educadoras para que sejam difusores de conhecimento para ação climática e, consequentemente, para que os estudantes se tornem agentes centrais para mudança de suas comunidades. A estratégia metodológica do Detetive Climático é baseada na educação dialógica de Paulo Freire, aprendizagem significativa e a Ciência Cidadã. Inclui atividades participativas e reflexão individual.
Contribuições de uma leitura fleckiana do movimento Anti-Vaxx para a compreensão do negacionismo científico
Authors: Rosa Marllon Moreti de Souza , Andrade Mariana Aparecida Bologna Soares de
Published: July 2024

This article addresses the career, professional interests, and socio-political context in which Ludwik Fleck lived and left its contributions. Next, it focuses on the concepts of “thinking style” and “thought collective” that are part of his epistemology. The paper aims to use them and other elements of Fleck’s epistemology to understand the historical-cultural processes involved in scientific denialism concerning the vaccine. To do this, we will use some historical examples. We consider that the anti-vaccine movement consists of a group of people who predominantly belong to exoteric circles in political terms, dogmatically expressing the political interests of these groups. Protesters against the vaccine position consider themselves defenders of individual freedom, distrusting the public machine and understanding the state as a controlling and invasive entity.
Editorial
Authors: Martins Lilian Al-Chueyr Pereira , Prestes Maria Elice de Brzezinski
Published: July 2024

Editorial da revista
Excess Mortality and Loss of Life Expectancy After Myocardial Infarction: A Registry-Based Matched Cohort Study
Authors: Reitan Christian , Andell Pontus , Alfredsson Joakim , Erlinge David , Hofmann Robin , Lindahl Bertil , Simonsson Moa , Dickman Paul W. , Jernberg Tomas
Published: July 2024

BACKGROUND:

The effect of myocardial infarction (MI) on life expectancy is difficult to study because the prevalence of MI hinders direct comparison with the life expectancy of the general population. We sought to assess this in relation to age, sex, and left ventricular ejection fraction (LVEF) by comparing individuals with MI with matched comparators without previous MI.

METHODS:

We included patients with a first MI between 1991 and 2022 from the nationwide SWEDEHEART registry (Swedish Web-System for Enhancement and Development of Evidence-Based Care in Heart Disease Evaluated According to Recommended Therapies), each matched with up to 5 comparators on age, sex, and region of residence. Flexible parametric survival models were used to estimate excess mortality and mean loss of life expectancy (LOLE) depending on index year, age, sex, and LVEF, and adjusted for differences in characteristics.

RESULTS:

A total of 335 748 cases were matched to 1 625 396 comparators. A higher LOLE was observed in younger individuals, women, and those with reduced LVEF (<50%). In 2022, the unadjusted and adjusted mean LOLE spanned from 11.1 and 9.5 years in 50-year-old women with reduced LVEF to 5 and 3.7 months in 80-year-old men with preserved LVEF. Between 1992 and 2022, the adjusted mean LOLE decreased by 36% to 55%: from 4.4 to 2.0 years and from 3.3 to 1.9 years in 50-year-old women and men, respectively, and from 1.7 to 1.0 years and from 1.4 to 0.9 years in 80-year-old women and men, respectively.

CONCLUSIONS:

LOLE is higher in younger individuals, women, and those with reduced LVEF, but is attenuated when adjusting for comorbidities and risk factors. Advances in MI treatment during the past 30 years have almost halved LOLE, with no clear sign of leveling off to a plateau.
Assessing climate niche similarity between persian fallow deer (Dama mesopotamica) areas in Iran
Authors: Rahimi Ehsan , Dong Pinliang , Ahmadzadeh Faraham
Published: July 2024

The Persian fallow deer or Mesopotamian fallow Deer (Dama mesopotamica, Brook 1875), a species of significant ecological importance, had faced the threat of extinction in Iran. One conservation strategy involved the translocation of Persian deer to enclosed areas across Iran, where they were afforded protection from external threats and provided with essential care by human caretakers. While human caretakers diligently attend to their needs and mitigate external threats, climate variables may now become critical factors affecting population dynamics in enclosed areas. This study aims to assess the similarity in climate niches between the original area (Dez and Karkheh) of the Persian deer species and 11 newly enclosed areas. To achieve this, we employed climate data and ecological niche modeling (ENM) techniques to assess the variations in climate among 12 areas. We utilized the environmental equivalency test to determine whether the environmental spaces of area pairs exhibit significant differences and whether these spaces are interchangeable. Extrapolation analyses were also constructed in the next steps to explore climatic conditions in original fallow deer habitats that are non-analogous to those in other parts of Iran. Our results reveal significant disparities in climate conditions between the original and all translocated areas. Based on observations of population growth in specific enclosed areas where translocated deer populations have thrived, we hypothesize that the species may demonstrate a non-equilibrium distribution in Iran. Consequently, these new areas could potentially be regarded as part of the species’ potential climate niche. Extrapolation analysis showed that for a significant portion of Iran, extrapolation predictions are highly uncertain and potentially unreliable for the translocation of Persian fallow deer. However, the primary objective of translocation efforts remains the establishment of self-sustaining populations of Persian deer capable of thriving in natural areas beyond enclosed areas, thus ensuring their long-term survival and contributing to preservation efforts. Evaluating the success of newly translocated species requires additional time, with varying levels of success observed. In cases where the growth rate of the species in certain enclosed areas falls below expectations, it is prudent to consider climate variables that may contribute to population declines. Furthermore, for future translocations, we recommend selecting areas with climate similarities to regions where the species has demonstrated growth rates.
Integrated analysis of DNA methylome and transcriptome revealing epigenetic regulation of CRIR1-promoted cold tolerance
Authors: Li Zhibo , Wang Wenjuan , Yu Xiaoling , Zhao Pingjuan , Li Wenbin , Zhang Xiuchun , Peng Ming , Li Shuxia , Ruan Mengbin
Published: July 2024

Background

DNA methylation contributes to the epigenetic regulation of nuclear gene expression, and is associated with plant growth, development, and stress responses. Compelling evidence has emerged that long non-coding RNA (lncRNA) regulates DNA methylation. Previous genetic and physiological evidence indicates that lncRNA-CRIR1 plays a positive role in the responses of cassava plants to cold stress. However, it is unclear whether global DNA methylation changes with CRIR1-promoted cold tolerance.

Results

In this study, a comprehensive comparative analysis of DNA methylation and transcriptome profiles was performed to reveal the gene expression and epigenetic dynamics after CRIR1 overexpression. Compared with the wild-type plants, CRIR1-overexpressing plants present gained DNA methylation in over 37,000 genomic regions and lost DNA methylation in about 16,000 genomic regions, indicating a global decrease in DNA methylation after CRIR1 overexpression. Declining DNA methylation is not correlated with decreased/increased expression of the DNA methylase/demethylase genes, but is associated with increased transcripts of a few transcription factors, chlorophyll metabolism and photosynthesis-related genes, which could contribute to the CRIR1-promoted cold tolerance.

Conclusions

In summary, a first set of transcriptome and epigenome data was integrated in this study to reveal the gene expression and epigenetic dynamics after CRIR1 overexpression, with the identification of several TFs, chlorophyll metabolism and photosynthesis-related genes that may be involved in CRIR1-promoted cold tolerance. Therefore, our study has provided valuable data for the systematic study of molecular insights for plant cold stress response.
Analytical and clinical performance evaluation of a new NT-proBNP assay
Authors: Feng Pingfeng , Qin Junlong , Chai Zhixin , Zhang Yajie , Zhao Minghai , Liu Liya , Zhang Lijun , Chen Yaqiong , Xiong Yufeng
Published: July 2024

Background

The study evaluated the performance of the Mindray N-terminal pro-B-type natriuretic peptide (NT-proBNP) in a healthy population in China, focusing on creating a reference range for future clinical applications adjusted according to different demographics.

Methods

The study measured NT-proBNP in 2277 healthy individuals. We analyzed age and sex-stratified data, performed precision, accuracy, linearitcvy, and detection limit studies, and evaluated method comparison and consistency between Roche and Mindray assays on 724 serum samples. We used Excel 2010, Medcalc, and GraphPad Prism 9.

Results

In males, the 97.5th centile NT-proBNP concentration at age < 45, 45 to 54, 55 to 64, 65 to 74 and ≧ 75 were 89.4 ng/L, 126 ng/L, 206 ng/L, 386 ng/L and 522 ng/L, respectively. In females, the concentration of NT-proBNP at the same age was 132 ng/L, 229 ng/L, 262 ng/L, 297 ng/L and 807 ng/L, respectively. The repeatability precision coefficient of variation (CV%) for NT-proBNP was between 0.86 and 1.65 in analytical performance. In contrast, the reproducibility precision (CV%) for NT-proBNP was between 1.52 and 3.22, respectively. The study found a bias of accuracy of 3.73% in low-value samples (concentration: 148.69) and 7.31% in high-value samples (concentration: 1939.08). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 125 ng/L were 96.6%, 92.3%, 84.2%, and 98.5%, respectively. In contrast, those of 300 ng/L were 94.0%, 98.2%, 95.7% and 97.5%, respectively.

Conclusions

The Mindray NT-proBNP assay showed increased levels in both males and females with age, with higher levels in women. It performs well and aligns with manufacturer specifications. We recommend adjusting cutoff values based on demographic factors.
A latent bleeding complication in ipsilateral common femoral puncture involving delayed diagnosis: a case report
Authors: Nomura Tetsuya , Yoshida Shiori , Kitamura Michitaka , Ono Kenshi , Shoji Keisuke , Kato Yukinori , Wada Naotoshi , Keira Natsuya , Tatsumi Tetsuya
Published: July 2024

Background

The trans femoral ipsilateral approach is often adopted for endovascular treatment (EVT) for better steerability of guidewires or better device deliverability. However, contrary to the trans femoral contralateral approach, ipsilateral antegrade puncture sometimes causes peculiar bleeding complications.

Case presentation

A 76-year-old female underwent EVT for chronic occlusion of the left superficial femoral artery (SFA) via the ipsilateral antegrade approach. After guidewire passage, we inflated the drug-coated balloons, but angiography showed blood flow stasis at the mid segment of the SFA. We also ensured prolonged balloon inflation, which resulted in favorable blood flow. While trying to ensure hemostasis, the blood pressure remained decreased, but neither bleeding nor superficial hematoma were observed at the puncture site. After hemostasis was achieved, we removed the surgical drape and noticed a swelling in the mid-portion of the thigh, distant from the puncture point. We then approached the left common femoral artery (CFA) contralaterally. Angiography showed continuous bleeding from a little bit distally to the sheath insertion point that was spreading through an intramuscular space. We stopped the bleeding with balloon tamponade inside the CFA. Angiography after hemostasis demonstrated blood flow stasis at the mid-segment of the SFA, similarly as that seen before. We confirmed compression of the SFA by a large hematoma using both intra- and extra- vascular ultrasound. Therefore, we deployed a self-expandable stent at the compressed SFA position. Finally, we achieved favorable blood flow on angiography.

Conclusion

We encountered a case that latent bleeding unrecognized in the surgical field persisted while prolonged inflation of DCB was conducted at just proximal SFA. We could have avoided bailout stenting by noticing the bleeding incident in a timely manner. Prediction and prevention are essential for all kinds of procedural complications in EVT.
Evaluating diabetes care in primary healthcare centers in Abuja, Nigeria: a cross-sectional formative assessment
Authors: Orji Ikechukwu A. , Baldridge Abigail S. , Ikechukwu-Orji Mercy U. , Banigbe Bolanle , Eze Nelson C. , Chopra Aashima , Omitiran Kasarachi , Iyer Guhan , Odoh Deborah , Alex-Okoh Morenike , Reng Rifkatu , Hirschhorn Lisa R. , Huffman Mark D. , Ojji Dike B.
Published: July 2024

Introduction

Noncommunicable diseases (NCDs) are associated with high and rising burden of morbidity and mortality in sub-Saharan Africa, including Nigeria. Diabetes mellitus (DM) is among the leading causes of NCD-related deaths worldwide and is a foremost public health problem in Nigeria. As part of National policy, Nigeria has committed to implement the World Health Organization (WHO) Package of Essential Non-communicable Disease interventions for primary care. Implementing the intervention requires the availability of essential elements, including guidelines, trained staff, health management information systems (HMIS), equipment, and medications, in primary healthcare centers (PHCs). This study assessed the availability of the DM component of the WHO package, and the readiness of the health workers in these PHCs to implement a DM screening, evaluation, and management program to inform future adoption and implementation.

Methods

This cross-sectional formative assessment adapted the WHO Service Availability and Readiness Assessment (SARA) tool to survey 30 PHCs selected by multistage sampling for readiness to deliver DM diagnosis and care in Abuja, Nigeria, between August and October 2021. The SARA tool was adapted to focus on DM services and the availability and readiness indicator scores were calculated based on the proportion of PHCs with available DM care services, minimum staff requirement, diagnostic tests, equipment, medications, and national guidelines/protocols for DM care within the defined SARA domain.

Results

All 30 PHCs reported the availability of at least two full-time staff (median [interquartile range] = 5 [4–9]), which were mostly community health extension workers (median [interquartile range]) = 3 [1–4]. At least one staff member was recently trained in DM care in 11 PHCs (36%). The study also reported high availability of paper-based HMIS (100%), and DM screening services using a glucometer (87%), but low availability of DM job aids (27%), treatment (23%), and national guidelines/protocols (0%).

Conclusion

This formative assessment of PHCs’ readiness to implement a DM screening, evaluation, and management program in Abuja demonstrated readiness to integrate DM care into PHCs regarding equipment, paper-based HMIS, and nonphysician health workers' availability. However, strategies are needed to promote DM health workforce training, provide DM management guidelines, and supply essential DM medications.
Change in suicidal ideation, depression, and anxiety following collaborative care in the community
Authors: Khazanov Gabriela Kattan , Wolk Courtney Benjamin , Lorenc Emily , Candon Molly , Pieri Matteo F. , Oslin David W. , Press Matthew J. , Anderson Eleanor , Famiglio Emilie , Jager-Hyman Shari
Published: July 2024

Background

The Collaborative Care Model (CoCM) increases access to mental health treatment and improves outcomes among patients with mild to moderate psychopathology; however, it is unclear how effective CoCM is for patients with elevated suicide risk.

Methods

We examined data from the Penn Integrated Care program, a CoCM program including an intake and referral management center plus traditional CoCM services implemented in primary care clinics within a large, diverse academic medical system. In this community setting, we examined: (1) characteristics of patients with and without suicidal ideation who initiated CoCM, (2) changes in suicidal ideation (Patient Health Questionnaire-9 [PHQ-9] item 9), depression (PHQ-9 total scores), and anxiety (Generalized Anxiety Disorder Scale-7 scores) from the first to last CoCM visit overall and across demographic subgroups, and (3) the relationship between amount of CoCM services provided and degree of symptom reduction.

Results

From 2018 to 2022, 3,487 patients were referred to CoCM, initiated treatment for at least 15 days, and had completed symptom measures at the first and last visit. Patients were 74% female, 45% Black/African American, and 45% White. The percentage of patients reporting suicidal ideation declined 11%-7% from the first to last visit. Suicidal ideation severity typically improved, and very rarely worsened, during CoCM. Depression and anxiety declined significantly among patients with and without suicidal ideation and across demographic subgroups; however, the magnitude of these declines differed across race, ethnicity, and age. Patients with suicidal ideation at the start of CoCM had higher depression scores than patients without suicidal ideation at the start and end of treatment. Longer CoCM episodes were associated with greater reductions in depression severity.

Conclusions

Suicidal ideation, depression, and anxiety declined following CoCM among individuals with suicidal ideation in a community setting. Findings are consistent with emerging evidence from clinical trials suggesting CoCM’s potential for increasing access to mental healthcare and improving outcomes among patients at risk for suicide.
Association and temporal sequence of pneumonia and gastrointestinal bleeding after acute ischemic stroke
Authors: Zhang Runhua , Hou Huiqing , Zhao Xingquan , Liu Liping , Wang Yilong , Liu Gaigen , Wang Yongjun , Ji Ruijun
Published: July 2024

Background

Stroke-associated pneumonia (SAP) and gastrointestinal bleeding (GIB) are common medical complications after stroke. The previous study suggested a strong association between SAP and GIB after stroke. However, little is known about the time sequence of SAP and GIB. In the present study, we aimed to verify the association and clarify the temporal sequence of SAP and GIB after ischemic stroke.

Methods

Patients with ischemic stroke from in-hospital Medical Complication after Acute Stroke study were analyzed. Data on occurrences of SAP and GIB during hospitalization and the intervals from stroke onset to diagnosis of SAP and GIB were collected. Multiple logistic regression was used to evaluate the association between SAP and GIB. Kruskal-Wallis test was used to compare the time intervals from stroke onset to diagnosis of SAP and GIB.

Results

A total of 1129 patients with ischemic stroke were included. The median length of hospitalization was 14 days. Overall, 86 patients (7.6%; 95% CI, 6.1-9.2%) developed SAP and 47 patients (4.3%; 95% CI, 3.0-5.3%) developed GIB during hospitalization. After adjusting potential confounders, SAP was significantly associated with the development of GIB after ischemic stroke (OR = 5.13; 95% CI, 2.02-13.00; P < 0.001). The median time from stroke onset to diagnosis of SAP was shorter than that of GIB after ischemic stroke (4 days vs. 5 days; P = 0.039).

Conclusions

SAP was associated with GIB after ischemic stroke, and the onset time of SAP was earlier than that of GIB. It is imperative to take precautions to prevent GIB in stroke patients with SAP.
Dental complications as a potential indicator of Redondovirus infection: a cross-sectional study
Authors: Mohebbi Alireza , Kiani Seyed Jalal , Khanaliha Khadijeh , Donyavi Tahereh , Emtiazi Nikoo , Sharifian Kimia , Mohebbi Maryam , Gholami Amytis , Behnezhad Farzane , Abbasi-Kolli Mohammad , Dehghani-Dehej Farzaneh , Bokharaei-Salim Farah
Published: July 2024

Background

Redondoviridae is a newly discovered virus family linked to oral and respiratory conditions in people, while there is still debate about whether it is also coinfected with other respiratory viruses. This study aimed to determine the frequency of Redondovirus (ReDoV) in nasopharyngeal samples and to investigate any possible links to SARS-CoV-2 infections.

Methods

A polymerase chain reaction (PCR) test was conducted on 731 nasopharyngeal samples from individuals referred to medical centers in Tehran, Iran, for SARS-CoV-2 testing to investigate the prevalence of ReDoV. An oral interview was performed to complete information on dental issues and the individuals’ demographics, symptoms, and vaccination history.

Results

The prevalence of ReDoV was 25.99%, and 15.26% had a coinfection with SARS-CoV-2. No notable correlation was found regarding ReDoVs and SARS-CoV-2 infections (p > 0.05). Women had a higher ReDoV positivity rate of 18.47% compared to men at 7.52% (p = 0.12), and there was no significant correlation between age groups and ReDoV presence. Nonetheless, a significant association was noted between ReDoVs and dental/gum issues (p < 0.0001, OR: 13.0326). A phylogenetic analysis showed that ReDoVs originated from various human-related clusters.

Conclusions

These results highlight the potential for detecting ReDoVs in nasopharyngeal samples of people with gum or dental issues. Additionally, conducting more ReDoV epidemiological research and proposing oral health as a possible marker for ReDoV infections is important.
Differentiation of glioma and solitary brain metastasis: a multi-parameter magnetic resonance imaging study using histogram analysis
Authors: Su Yifei , Cheng Rui , Guo Jinxia , Zhang Miaoqi , Wang Junhao , Ji Hongming , Wang Chunhong , Hao Liangliang , He Yexin , Xu Cheng
Published: July 2024

Background

Differentiation of glioma and solitary brain metastasis (SBM), which requires biopsy or multi-disciplinary diagnosis, remains sophisticated clinically. Histogram analysis of MR diffusion or molecular imaging hasn’t been fully investigated for the differentiation and may have the potential to improve it.

Methods

A total of 65 patients with newly diagnosed glioma or metastases were enrolled. All patients underwent DWI, IVIM, and APTW, as well as the T1W, T2W, T2FLAIR, and contrast-enhanced T1W imaging. The histogram features of apparent diffusion coefficient (ADC) from DWI, slow diffusion coefficient (Dslow), perfusion fraction (frac), fast diffusion coefficient (Dfast) from IVIM, and MTRasym@3.5ppm from APTWI were extracted from the tumor parenchyma and compared between glioma and SBM. Parameters with significant differences were analyzed with the logistics regression and receiver operator curves to explore the optimal model and compare the differentiation performance.

Results

Higher ADCkurtosis (P = 0.022), frackurtosis (P<0.001),and fracskewness (P<0.001) were found for glioma, while higher (MTRasym@3.5ppm)10 (P = 0.045), frac10 (P<0.001),frac90 (P = 0.001), fracmean (P<0.001), and fracentropy (P<0.001) were observed for SBM. frackurtosis (OR = 0.431, 95%CI 0.256–0.723, P = 0.002) was independent factor for SBM differentiation. The model combining (MTRasym@3.5ppm)10, frac10, and frackurtosis showed an AUC of 0.857 (sensitivity: 0.857, specificity: 0.750), while the model combined with frac10 and frackurtosis had an AUC of 0.824 (sensitivity: 0.952, specificity: 0.591). There was no statistically significant difference between AUCs from the two models. (Z = -1.14, P = 0.25).

Conclusions

The frac10 and frackurtosis in enhanced tumor region could be used to differentiate glioma and SBM and (MTRasym@3.5ppm)10 helps improving the differentiation specificity.
Exploring the shift in newborn care practices among mothers and grandmothers in rural Odisha, India — a qualitative study
Authors: Thomas Livson , Arun Sumitha , Varughese Deepak Thomas , Soreng Christ Kamalini , Manjari B. Prativa , Khosla Prabhati S. , Pani Dikhita K.
Published: July 2024

Background

This study delves into newborn care and infant-feeding practices in rural Odisha, specifically focusing on the rural KBK + region of Odisha (Kalahandi-Bolangir-Koraput region), inhabited predominantly by Schedule Tribes and Schedule Castes individuals. There has been an improvement in the health indicators in these areas in recent times. In the background of improved health indicators in these areas, this research explores the current and changing newborn care practices and attempts to gain insight into people’s perceptions of the factors that brought about the changes.

Methods

This qualitative study was conducted between February and July 2023 at Christian Hospital, Bissamcuttack in Odisha. The methodology involved focused group discussions with mothers and grandmothers.

Results

Analysis revealed healthy practices like exclusive breastfeeding till six months of age, appropriate timing of the first bath, and prompt healthcare-seeking behavior for minor illnesses among the mothers. The use of cow ghee or breast milk in a baby’s eyes, the application of ash on the umbilical cord, and the use of herbal medicines for minor illnesses were practiced more by the grandmothers in the past and were not as popular among the mothers. It is noteworthy that the cultural practices to ward off the ‘evil eye’ were practiced by both mothers and grandmothers alike. Despite the influence of traditional cultural practices on the beliefs and norms of the community, the study identified a shift in health-seeking behavior, with increased reliance on healthcare providers and safe healthcare practices. The study identifies the pivotal role of Accredited Social Health Activists (ASHAs) as a bridge between the rural communities and the healthcare system.

Conclusions

This research provides valuable insights for healthcare providers aiming to enhance community-centric safe newborn care practices in rural settings. The emphasis is on the importance of understanding the current and changing local practices. This would help the healthcare providers to encourage healthy practices while eliminating the harmful practices related to newborn care through community workers like ASHA and Anganwadi workers.
Socioeconomic determinants and reasons for non-acceptance to vaccination recommendations during the 3rd - 5th waves of the COVID-19 pandemic in Hungary
Authors: Ligeti Anna Sára , Oroszi Beatrix , Luca Csaba , Bilics Edit , Ágoston József , Röst Gergely , Koltai Júlia
Published: July 2024

Background

In Hungary, although six types of vaccines were widely available, the percentage of people receiving the primary series of COVID-19 vaccination remained below the EU average. This paper investigates the reasons for Hungary’s lower vaccination coverage by exploring changing attitudes towards vaccination, socio-demographic determinants, and individual reasons for non-acceptance during the 3rd - 5th pandemic waves of COVID-19.

Methods

The study’s empirical analysis is based on representative surveys conducted in Hungary between February 19, 2021, and June 30, 2022. The study used a total of 17 surveys, each with a sample size of at least 1000 respondents. Binomial logistic regression models were used to investigate which socio-demographic characteristics are most likely to influence vaccine hesitancy in Hungary. The study analysed 2506 open-ended responses to identify reasons for vaccine non-acceptance. The responses were categorised into four main categories and 13 sub-categories.

Results

Between the third and fifth wave of the pandemic, attitudes towards COVID-19 vaccination have significantly changed. Although the proportion of vaccinated individuals has increased steadily, the percentage of individuals who reported not accepting the vaccine has remained almost unchanged. Socio-demographic characteristics were an important determinant of the observed vaccine hesitancy, although they remained relatively stable over time. Individuals in younger age groups and those with lower socioeconomic status were more likely to decline vaccination, while those living in the capital city were the least likely. A significant reason behind vaccine refusal can undoubtedly be identified as lack of trust (specifically distrust in science), facing an information barrier and the perception of low personal risk.

Conclusion

Although compulsory childhood vaccination coverage is particularly high in Hungary, voluntary adult vaccines, such as the influenza and COVID-19 vaccines, are less well accepted. Vaccine acceptance is heavily affected by the social-demographic characteristics of people. Mistrust and hesitancy about COVID-19 vaccines, if not well managed, can easily affect people’s opinion and acceptance of other vaccines as well. Identifying and understanding the complexity of how vaccine hesitancy evolved during the pandemic can help to understand and halt the decline in both COVID-19 and general vaccine confidence by developing targeted public health programs to address these issues.
Factors related with lung functions among Orang Asli in Tasik Chini, Malaysia: a cross-sectional study
Authors: Mohd Razib Nur Fadhilah , Ismail Halim , Ibrahim Roszita , Isa Zaleha Md
Published: July 2024

Background

Orang Asli lifestyle and household setting may influence their health status especially respiratory system and lung functions. This cross-sectional study was carried out to investigate the status of lung functions of Orang Asli community and the associated factors.

Methods

Data collection was carried out from November 2017 until May 2018 among 211 Orang Asli respondents aged 18 years old and above, who lived in five villages in Tasik Chini, Pahang. All respondents who fulfilled the inclusion criteria were recruited in this study. Interview-guided questionnaire was administered, and spirometry test that include Forced Expiratory Volume in one second (FEV1), Forced Vital Capacity (FVC), and Peak Expiratory Flow Rate (PEFR) was carried out. Data were analyzed using SPSS software version 23.0. In the first stage, descriptive analysis was done to describe the characteristics of the respondents. In the second stage, bivariable analysis was carried out to compare proportions. Finally, multiple logistic regression was performed to assess the effects of various independent predictors on spirometry parameters.

Results

The respondents’ age ranged from 18 to 71 years old in which 50.2% of them were female. The majority ethnicity in Tasik Chini was Jakun tribe (94.3%). More than half of the respondents (52.1%) were current smoker, 5.2% were ex-smoker and 41.7% were non-smoker. More than half of them (62.1%) used woodstove for cooking, compared to only 37.9% used cleaner fuel like Liquefied Petroleum Gas (LPG) as a fuel for everyday cooking activity. The lung function parameters (FEV1 and FVC) were lower than the predictive value, whereas the ratio of Forced Expiratory Volume in one second and Forced Vital Capacity (FEV1/FVC) (%) and PEFR were within the predictive value. The FEV1 levels were significantly associated with age group (18–39 years old) (p = 0.002) and presence of woodstove in the house (p = 0.004). FVC levels were significantly associated with presence of woodstove in the house (p = 0.004), whereas there were no significant associations between all factors and FEV1/FVC levels.

Conclusions

FEV1 levels were significantly associated with age group 18–39 years old, whereas FVC levels were significantly associated with the presence of woodstove in the house. Thus, environmental interventions such as replacing the use of woodstove with LPG, need to be carried out to prevent further worsening of respiratory health among Orang Asli who lived far from health facilities. Moreover, closer health monitoring is crucial especially among the younger and productive age group.