bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Air Taxis on Air Traffic in the Vicinity of Airports
Authors: Ahrenhold Nils , Pohling Oliver , Schier-Morgenthal Sebastian
Published: October 2021

The extension of the current urban transportation system utilising the third dimension by air taxi (AT) operations represents a potential solution for the congestion of metropolitan areas. A major asset for AT operations is the connection to existing airports enabling the access to multiple other transportation systems. This paper develops an analytical model for AT operations and their capacity impact on airports, exemplary for Hamburg airport. The model is developed, based on the results of a fast time simulation (FTS) considering multiple aspects, such as vehicle configuration and touchdown and lift-off areas (TLOF). Collectively, three integration methods were analysed, each of them impacting the conventional air traffic differently. The results show that an integration using the runway-system is not possible with five ATs per hour. Further methods allow an integration of up to 20 air taxis per hour. Additionally, an energy consumption analysis of the ATs is conducted. Finally, proposals are given for integrating ATs at an airport and further strategies to extend the analytical model. Through this work, a model to calculate and predict an AT’s influence on the airside capacity of an airport is designed. This is an important step for the practical implementation of AT operations at airports.
Facile Synthesis and Redox Behavior of an Overcrowded Spirogermabifluorene
Authors: Morisako Shogo , Noro Kohsuke , Sasamori Takahiro
Published: October 2021

A spirogermabifluorene that bears sterically demanding 3,3′,5,5′-tetra(t-butyl)-2,2′-biphenylene groups (1) was obtained from the reaction of in-situ-generated 2,2′-dilithiobiphenylene with GeCl2·(dioxane). The solid-state structure and the redox behavior of 1 were examined by single-crystal X-ray diffraction analysis and electrochemical measurements, respectively. The sterically hindered biphenyl ligands endow 1 with high redox stability and increased electron affinity. The experimental observations were corroborated by theoretical DFT calculations.
Towards Integrated Pest and Pollinator Management in Intensive Pear Cultivation: A Case Study from Belgium
Authors: Belien Tim , Raymaekers Stijn , Eeraerts Maxime , Mommaerts Veerle , Claus Gregor , Bogen Christian , Piot Niels , Smagghe Guy , Spanoghe Pieter , Bylemans Dany
Published: October 2021

Recently, the concept of Integrated Pest Management (IPM) was further extended into Integrated Pest and Pollinator Management (IPPM). Implementation of IPPM strategies entails the combination of actions for pest and pollinator management providing complementary or synergistic benefits for yield and/or quality of the harvest. The aim of this study was to examine IPPM elements (i.e., mixed hedgerow, nesting boxes for mason bees, Osmia spp.) and demonstrate their impact in the practical context of modern commercial fruit cultivation in a 4-year case study in an intensive ‘Conference’ pear orchard. The outcomes of visual observations during transect walks and molecular analysis of pollen collected by mason bees, showed the importance of additional floral resources for the presence of mason bees and other pollinating insects in the orchard environment. Pear quality assessments indicated that insect-mediated pollination had a significant positive impact, with a tendency for higher quality pears in the close vicinity of Osmia nesting boxes. However, despite the fact that pear pollen was also detected in Osmia spp. nest cells, the amount and frequency of pear pollen collection for their nest built-up turned out to be rather low. In the same intensive pear orchard studied for pollination effects, we simultaneously demonstrate the impact of a mixed hedgerow to enhance integrated pest control.
Preoperative Assessment and Management of Cardiovascular Risk in Patients Undergoing Non-Cardiac Surgery: Implementing a Systematic Stepwise Approach during the COVID-19 Pandemic Era
Authors: Bossone Eduardo , Cademartiri Filippo , AlSergani Hani , Chianese Salvatore , Mehta Rahul , Capone Valentina , Ruotolo Carlo , Tarrar Imran Hayat , Frangiosa Antonio , Vriz Olga , Maffei Vincenzo , Annunziata Roberto , Galzerano Domenico , Ranieri Brigida , Sepe Chiara , Salzano Andrea , Cocchia Rosangela , Majolo Massimo , Russo Giuseppe , Longo Giuseppe , Muto Mario , Fedelini Paolo , Esposito Ciro , Perrella Alessandro , Guggino Gianluca , Raiola Eliana , Catalano Mara , De Palma Maurizio , Romano Luigia , Romano Gaetano , Coppola Ciro , Mauro Ciro , Mehta Rajendra H.
Published: October 2021

Major adverse cardiac events, defined as death or myocardial infarction, are common causes of perioperative mortality and major morbidity in patients undergoing non-cardiac surgery. Reduction of perioperative cardiovascular risk in relation to non-cardiac surgery requires a stepwise patient evaluation that integrates clinical risk factors, functional status and the estimated stress of the planned surgical procedure. Major guidelines on preoperative cardiovascular risk assessment recommend to establish, firstly, the risk of surgery per se (low, moderate, high) and the related timing (elective vs. urgent/emergent), evaluate the presence of unstable cardiac conditions or a recent coronary revascularization (percutaneous coronary intervention or coronary artery bypass grafting), assess the functional capacity of the patient (usually expressed in metabolic equivalents), determine the value of non-invasive and/or invasive cardiovascular testing and then combine these data in estimating perioperative risk for major cardiac adverse events using validated scores (Revised Cardiac Risk Index (RCRI) or National Surgical Quality Improvement Program (NSQIP)). This stepwise approach has the potential to guide clinicians in determining which patients could benefit from cardiovascular therapy and/or coronary artery revascularization before non-cardiac surgery towards decreasing the incidence of perioperative morbidity and mortality. Finally, it should be highlighted that there is a need to implement specific strategies in the 2019 Coronavirus disease (COVID-19) pandemic to minimize the risk of transmission of COVID-19 infection during the preoperative risk assessment process.
Acute and Chronic Effects of COVID-19 on the Cardiovascular System
Authors: Arévalos Victor , Ortega-Paz Luis , Rodríguez-Arias Juan José , Calvo López Margarita , Castrillo-Golvano Leticia , Salazar-Rodríguez Anthony , Sabaté-Tormos Marta , Spione Francesco , Sabaté Manel , Brugaletta Salvatore
Published: October 2021

COVID-19 has shown significant morbidity with the involvement of multiple systems, including the cardiovascular system. Cardiovascular manifestations in the acute phase can include myocardial injury itself, myocardial infarction, venous thromboembolic events, myocarditis, Takotsubo syndrome, and different arrhythmic events. Myocardial injury defined by the rise of cardiac biomarkers in blood has been found in multiple studies with a prevalence of about 20%. Its presence is related to worse clinical outcomes and in-hospital mortality. The mechanisms of myocardial injury have been the subject of intense research but still need to be clarified. The characterization of the cardiac affectation with echocardiography and cardiac magnetic resonance has found mixed results in different studies, with a striking incidence of imaging criteria for myocarditis. Regarding post-acute and chronic follow-up results, the persistence of symptoms and imaging changes in recovered COVID-19 patients has raised concerns about the duration and the possible significance of these findings. Even though the knowledge about this disease has increased incredibly in the last year, many aspects are still unclear and warrant further research.
Speckle-Tracking Global Longitudinal and Regional Strain Analysis in Neonates with Coarctation of Aorta: A Case-Control Study
Authors: Gozar Liliana , Mărginean Claudiu , Cerghit Paler Andreea , Gabor-Miklosi Dorottya , Toma Daniela , Iancu Mihaela , Togănel Rodica , Făgărășan Amalia
Published: October 2021

Our objectives are to compare speckle-tracking peak global longitudinal (pGLS) and regional strain values in neonates with coarctation of aorta (CoA) and control groups. Echocardiographic parameters measured by speckle-tracking were studied in a retrospective single-center study. A comparison of pGLS and segmental deformation between neonates with CoA and control group was performed using a three-way mixed ANOVA model. There was a significant difference in the means of segmental strain values between CoA and control group at the apical (p = 0.018) and basal segments (p = 0.031) of the interventricular septum and at the apical segment (p = 0.026) of the left ventricle (LV). After correcting for multiple comparisons, the results had a tendency toward statistical significance (adjusted-p < 0.10). There was significant difference in the mean values of pGLS [F(1, 39) = 7.61, p = 0.009, adjusted p = 0.018] between the studied groups. The results of ROC analysis showed that a cut-off value of −16.60% for pGLS provided an estimated sensitivity of 92.31% (95% CI: [63.97, 99.81]) and 71.43% specificity (95% CI: [51.33, 86.78]) for the diagnosis of CoA in neonates (AUC = 0.794, 95% CI: [0.66, 0.93]). pGLS can be regarded as a feasible and reproducible parameter reflecting LV dysfunction in newborns with CoA when compared to newborns with a false-positive diagnosis.
PD-L1 Inhibitors as Monotherapy for the First-Line Treatment of Non-Small-Cell Lung Cancer in PD-L1 Positive Patients: A Safety Data Network Meta-Analysis
Authors: García Campelo María Rosario , Arriola Edurne , Campos Balea Begoña , López-Brea Marta , Fuentes-Pradera José , de Castro Carpeno Javier , Aguado Carlos , Pérez Parente Diego , de Oro Pulido Fidel , Ruiz-Gracia Pedro , Rodríguez-Abreu Delvys
Published: October 2021

This network meta-analysis (NMA) evaluates the safety of first-line programmed death-ligand 1 (PD-L1) inhibitor monotherapy in advanced NSCLC patients compared to platinum-based chemotherapy. We also compared the risk of adverse events (AEs) according to programmed cell death-1 receptor (PD-1) or PD-L1 inhibitors therapy. To that end, we conducted a series of metanalyses (MAs) using data from six phase III clinical trials, including 4053 patients. Our results show a reduced risk of any grade treatment-related AEs (risk ratio (RR) = 0.722 95% CI: 0.667–0.783, p = 0.002), and grade 3–5 AEs (RR = 0.406 95% CI: 0.340–0.485, p = 0.023) in immunotherapy as compared to chemotherapy. In contrast, a higher risk of immune-related AEs (irAEs) was estimated for immunotherapy versus chemotherapy. The subgroup MAs comparing PD-L1 to PD-1 inhibitors, determined a lower risk of AEs leading to treatment discontinuation in the anti-PD-L1 subgroup (RR = 0.47 95% CI: 0.29–0.75, p = 0.001); however, this statistically significant difference between anti-PD-L1 and anti-PD-1 subgroups was not reached for other safety outcomes analyzed. In conclusion, our findings show that PD-L1 inhibitor monotherapy improves safety outcomes in the 1L treatment of advanced NSCLC patients as compared to chemotherapy except for irAEs.
The Potential Role of Proinflammatory Cytokines and Complement Components in the Development of Drug-Induced Neuropathy in Patients with Multiple Myeloma
Authors: Łuczkowska Karolina , Rutka Magdalena , Rogińska Dorota , Paczkowska Edyta , Baumert Bartłomiej , Milczarek Sławomir , Górska Martyna , Kulig Piotr , Osękowska Bogumiła , Janowski Michał , Safranow Krzysztof , Sommerfeld Krzysztof , Borowiecka Ewa , Zawodny Piotr , Koclęga Anna , Helbig Grzegorz , Machaliński Bogusław
Published: October 2021

The launch of novel chemotherapeutic agents—in particular, proteasome inhibitors and immunomodulatory drugs—dramatically changed multiple myeloma (MM) therapy, improving the response rate and prolonging progression-free survival. However, none of the anti-MM drugs are deprived of side effects. Peripheral neuropathy (PN) seems to be one of the most pressing problems. Despite extensive research in this area, the pathogenesis of drug-induced peripheral neuropathy (DiPN) has not yet been fully elucidated. In the present study, we aimed to assess the potential relationship between proinflammatory factors and the development of PN in MM patients with particular emphasis on the application of VTD (bortezomib, thalidomide, dexamethasone) regimen. Our analysis identified increased concentrations of CCL2, IL-1β, and IFN-γ in plasma of MM patients during treatment, both with and without symptoms of PN, compared with untreated neuropathy-free MM patients. At the same time, the plasma concentration of IL-1β in patients with neuropathy was significantly increased compared with patients without PN before and during treatment. Moreover, the results were enhanced at the transcript level by performing global mRNA expression analysis using microarray technology. The most significant changes were observed in the expression of genes responsible for regulating immunological and apoptotic processes. An in-depth understanding of the mechanisms responsible for the development of DiPN might in the future reduce the incidence of PN and accelerate diagnosis, allowing the choice of neuropathy-free treatment strategies for MM.
Identifying Common Pathogenic Features in Deep Endometriotic Nodules and Uterine Adenomyosis
Authors: Stratopoulou Christina Anna , Camboni Alessandra , Donnez Jacques , Dolmans Marie-Madeleine
Published: October 2021

Increasing imaging data point to a link between deep endometriotic nodules (DENs) and uterine adenomyosis (AD). The study aimed to investigate this link at the histological level and detect potential features shared by the two diseases. We collected formalin-fixed paraffin-embedded tissue (endometrium and lesions) from women with DENs of the rectovaginal septum (n = 13), AD (n = 14), and control subjects (n = 14). Immunohistochemical analyses of CD41 and CD68 were conducted to explore the roles of platelets and macrophages, respectively. Picrosirius red staining was carried out to gather evidence of fibrosis. Vascular endothelial growth factor (VEGF) was assessed, and total numbers of CD31-positive vessels were calculated to investigate the mechanism governing angiogenesis. Double immunohistochemistry for CD31 and alpha smooth muscle actin (αSMA) was performed to discern stable vessels. Platelet aggregation was significantly decreased in both types of lesions compared to their corresponding eutopic endometrium and healthy controls. Macrophage numbers were higher in both lesions than in their corresponding endometrium and healthy subjects. Significantly higher rates of collagen accumulation were detected in DENs and AD lesions compared to their corresponding eutopic and healthy endometrium. VEGF expression was downregulated in the stromal compartment of AD lesions compared to the healthy endometrium. The total number of vessels per area was significantly higher in DENs and AD lesions than in the healthy endometrium. Rates of αSMA-surrounded vessels were decreased in DENs and AD lesions compared to their corresponding eutopic and healthy endometrium. We report common pathogenic mechanisms between DENs and AD, namely excessive macrophage accumulation, fibrosis, and irregular angiogenesis. Our results further support the notion of DENs and AD being linked at the histological level.
Radiological Evaluation of Cervical Spine Involvement in Rheumatoid Arthritis: A Cross-Sectional Retrospective Study
Authors: Kotecki Mateusz , Gasik Robert , Głuszko Piotr , Sudoł-Szopińska Iwona
Published: October 2021

Background: Cervical spine lesions are a common manifestation of rheumatoid arthritis (RA). The purpose of this study was to conduct a retrospective analysis of radiological lesions in cervical spine in patients with RA and to correlate findings with clinical and laboratory parameters. Methods: Overall, 240 consecutive patients with RA were referred for imaging by clinicians based on symptoms suggesting cervical spine involvement and/or long disease duration. In each patient, lateral radiographs and MRI of the cervical spine were performed. The imaging data were correlated with clinical records and laboratory data. Results: The cervical spine was affected in 179 patients (75%). The most common lesions were anterior atlanto-axial subluxation (AAS; 58%), subaxial subluxation (58%), and demineralization (48%). Cervical spine involvement was linked to longer disease duration (p = 0.007), the presence of rheumatoid factor (RF; p = 0.010), elevated C-reactive protein (CRP) levels (p = 0.016), and accelerated erythrocyte sedimentation rate (ESR; p = 0.025). Longer disease duration was associated with anterior AAS (p = 0.005), subaxial subluxation (p = 0.005), and basilar settling (p = 0.003). Conclusions: As many as 75% of RA patients develop lesions that can be observed on radiographs and through MRI. The most frequent radiological findings include anterior AAS and subaxial subluxation. Long disease duration, RF seropositivity, and elevated inflammatory markers were risk factors for cervical spine involvement.
A Surveillance Endoscopy Strategy Based on Local Recurrence Rates after Colorectal Endoscopic Submucosal Dissection
Authors: Park Jin Hwa , Yoon Ji Young , Hwang Sung Wook , Park Sang Hyoung , Yang Dong-Hoon , Ye Byong Duk , Myung Seung-Jae , Yang Suk-Kyun , Byeon Jeong-Sik
Published: October 2021

Backgrounds: It is not clear when and how frequently surveillance endoscopy should be performed after colorectal endoscopic submucosal dissection (ESD). We aimed to suggest a surveillance endoscopy strategy by investigating the cumulative local recurrence rates and identifying risk factors for local recurrence after colorectal ESD. Methods: We reviewed the medical records of 770 patients who underwent colorectal ESD for 778 lesions at our institution from 2005 to 2016. We investigated the cumulative local recurrence rates and risk factors for local recurrence. Results: Local recurrence developed in 12 (1.5%) of 778 lesions during the follow-up period of 37.4 ± 31.7 months. The one-, three-, and five-year cumulative local recurrence rates were 0.4%, 1.7%, and 2.2%, respectively. The risk factors for local recurrence were piecemeal resection (odds ratio (OR) 3.948, 95% confidence interval (CI) 1.164–13.385; p = 0.028) and histological incomplete resection (OR 8.713, 95% CI 2.588–29.334; p < 0.001). Local recurrence tended to develop frequently after ESD of early cancers. Conclusions: Short-term surveillance endoscopy should be recommended after piecemeal ESD, histological incomplete resection, and ESD of early colorectal cancers. Surveillance endoscopy with longer intervals can be suggested after en bloc ESD with the histological complete resection of benign colorectal tumors.
Acute Kidney Disease and Mortality in Acute Kidney Injury Patients with COVID-19
Authors: Marques Filipe , Gameiro Joana , Oliveira João , Fonseca José Agapito , Duarte Inês , Bernardo João , Branco Carolina , Costa Claúdia , Carreiro Carolina , Braz Sandra , Lopes José António
Published: October 2021

Background: The incidence of AKI in coronavirus disease 2019 (COVID-19) patients is variable and has been associated with worse prognosis. A significant number of patients develop persistent kidney damage defined as Acute Kidney Disease (AKD). There is a lack of evidence on the real impact of AKD on COVID-19 patients. We aim to identify risk factors for the development of AKD and its impact on mortality in COVID-19 patients. Methods: Retrospective analysis of COVID-19 patients with AKI admitted at the Centro Hospitalar Universitário Lisboa Norte between March and August of 2020. The Kidney Disease Improving Global Outcomes (KDIGO) classification was used to define AKI. AKD was defined by presenting at least KDIGO Stage 1 criteria for >7 days after an AKI initiating event. Results: In 339 COVID-19 patients with AKI, 25.7% patients developed AKD (n = 87). The mean age was 71.7 ± 17.0 years, baseline SCr was 1.03 ± 0.44 mg/dL, and the majority of patients were classified as KDIGO stage 3 AKI (54.3%). The in-hospital mortality was 18.0% (n = 61). Presence of hypertension (p = 0.006), CKD (p < 0.001), lower hemoglobin (p = 0.034) and lower CRP (p = 0.004) at the hospital admission and nephrotoxin exposure (p < 0.001) were independent risk factors for the development of AKD. Older age (p = 0.003), higher serum ferritin at admission (p = 0.008) and development of AKD (p = 0.029) were independent predictors of in-hospital mortality in COVID-19-AKI patients. Conclusions: AKD was significantly associated with in-hospital mortality in this population of COVID-19-AKI patients. Considering the significant risk of mortality in AKI patients, it is of paramount importance to identify the subset of higher risk patients.
Biomechanical Value of a Protective Proximal Humeral Cerclage in Reverse Total Shoulder Arthroplasty
Authors: Michel Philipp A. , Katthagen J. Christoph , Schliemann Benedikt , Wilkens Sina , Frank Andre , Heilmann Lukas F. , Dyrna Felix , Raschke Michael J.
Published: October 2021

Reverse shoulder arthroplasty (RSA) is a commonly performed salvage procedure for failed proximal humeral fracture fixation. The rate of intraoperative periprosthetic fractures is higher compared to primary RSA. The goal of this study was to investigate the biomechanical value of a protective cerclage during stem impaction in a revision surgery setting. Twenty-eight fresh-frozen human humeri were used to assess different configurations for steel wire and FiberTape cerclages. A custom-built biomechanical test setup simulated the mallet strikes during the stem impaction process with the Univers Revers prothesis stem. The mallet energy until the occurrence of a first crack was not different between groups. The total energy until progression of the fracture distally to the cerclage was significantly higher in the cerclage groups compared to the native humerus (9.5 J vs. 3.5 J, respectively; p = 0.0125). There was no difference between the steel wire and FiberTape groups (11.4 J vs. 8.6 J, respectively; p = 0.2695). All fractures were located at the concave side of the stem at the metaphyseal calcar region. This study demonstrates that a protective cerclage can successfully delay the occurrence of a fracture during stem impaction in reverse shoulder arthroplasty. A FiberTape cerclage is biomechanically equally efficient compared to a steel wire cerclage.
Masked Face Analysis via Multi-Task Deep Learning
Authors: Patel Vatsa S. , Nie Zhongliang , Le Trung-Nghia , Nguyen Tam V.
Published: October 2021

Face recognition with wearable items has been a challenging task in computer vision and involves the problem of identifying humans wearing a face mask. Masked face analysis via multi-task learning could effectively improve performance in many fields of face analysis. In this paper, we propose a unified framework for predicting the age, gender, and emotions of people wearing face masks. We first construct FGNET-MASK, a masked face dataset for the problem. Then, we propose a multi-task deep learning model to tackle the problem. In particular, the multi-task deep learning model takes the data as inputs and shares their weight to yield predictions of age, expression, and gender for the masked face. Through extensive experiments, the proposed framework has been found to provide a better performance than other existing methods.
Food-Grade Biorefinery Processing of Macroalgae at Scale: Considerations, Observations and Recommendations
Authors: Adams Jessica M. M. , Morris S. Michael , Steege Laura , Robinson Joanne , Bavington Charles
Published: October 2021

Using brown seaweed kelp species Saccharina latissima and Laminaria digitata as feedstocks, a set of pilot-scale macroalgae processing batches were conducted (50–200 kg per batch) for the production of a range of food-grade liquid and solid fractions. The aim of this communication is to relay a number of lessons learnt during this period in combination with previous relevant observations and considerations for others who are intending to process macroalgae at scale. The novelty of this paper is thus to form a bridge between academic findings and practical know-how. Considerations covers material diversity; abiotic and biotic impact and variation; and supply chain considerations. Observations covers milling and cutting; equipment requirements; and acids including their effects on heavy metals, especially lead. Recommendations summarises key points from this pilot-scale and previous work. These include: harvest seasonality, water quality and proximity to processing facilities; minimising contaminants within the macroalgae such as stones and shells; considering equipment composition and volume for all steps and processes including final product quality; acid choice and its effects on both the equipment used and the metals bioaccumulated within the macroalgae.
The Depositional Environments in the Cilento Offshore (Southern Tyrrhenian Sea, Italy) Based on Marine Geological Data
Authors: Aiello Gemma , Caccavale Mauro
Published: October 2021

The depositional environments offshore of the Cilento Promontory have been reconstructed based on the geological studies performed in the frame of the marine geological mapping of the geological sheet n. 502 “Agropoli”. The littoral environment (toe-of-coastal cliff deposits and submerged beach deposits), the inner continental shelf environment (inner shelf deposits and bioclastic deposits), the outer continental shelf environment (outer shelf deposits and bioclastic deposits), the lowstand system tract and the Pleistocene relict marine units have been singled out. The littoral, inner shelf and outer shelf environments have been interpreted as the highstand system tract of the Late Quaternary depositional sequence. This sequence overlies the Cenozoic substratum (ssi unit), composed of Cenozoic siliciclastic rocks, genetically related with the Cilento Flysch. On the inner shelf four main seismo-stratigraphic units, overlying the undifferentiated acoustic basement have been recognized based on the geological interpretation of seismic profiles. On the outer shelf, palimpsest deposits of emerged to submerged beach and forming elongated dunes have been recognized on sub-bottom profiles and calibrated with gravity core data collected in previous papers. The sedimentological analysis of sea bottom samples has shown the occurrence of several grain sizes occurring in this portion of the Cilento offshore.
Designing the Next Generation of Condition Tracking and Early Warning Systems for Shellfish Aquaculture
Authors: Guarini Jean-Marc , Hinz Shawn , Coston-Guarini Jennifer
Published: October 2021

Early detection of environmental disturbances affecting shellfish stock condition is highly desirable for aquaculture activities. In this article, a new biophysical model-based early warning system (EWS) is described, that assesses bivalve stock condition by diagnosing signs of persistent physiological dysfunctioning. The biophysical model represents valve gape dynamics, controlled by active contractions of the adductor muscle countering the passive action of the hinge ligament; the dynamics combine continuous convergence to a steady-state interspersed with discrete closing events. A null simulation was introduced to describe undisturbed conditions. The diagnostic compares valve gape measurements and simulations. Indicators are inferred from the model parameters, and disturbances are assessed when their estimates deviate from their null distribution. Instead of focusing only on discrete events, our EWS exploits the complete observed dynamics within successive time intervals defined by the variation scales. When applied to a valvometry data series, collected in controlled conditions from scallops (Pecten maximus), the EWS indicated that one among four individuals exhibited signs its physiological condition was degrading. This was detected neither during experiments nor during the initial data analysis, suggesting the utility of an approach that quantifies physiological mechanisms underlying functional responses. Practical implementations of biological-EWS at farming sites are then discussed.
Exploring the Current Practices and Future Needs of Marine Engineering Education in Bangladesh
Authors: Tusher Hasan Mahbub , Sharma Amit , Nazir Salman , Munim Ziaul Haque
Published: October 2021

Developing countries, despite being the major suppliers of maritime manpower, lag in their future-proof competence development. This study explores whether the current maritime education and training (MET) practices for marine engineering education in a developing nation are perceived as sufficient by the early-stage marine engineers. The current competence requirements set by the Standards of Training, Certification and Watchkeeping for Seafarers (STCW’74 as amended) are compared and contrasted against the current MET practices as well as the future needs for remotely controlled autonomous ship operations. Bangladesh, a maritime nation in the Southeast Asia region was selected as a developing nation for this study. An online survey was conducted among early-stage professional marine engineers. The analysis of 62 valid responses revealed that current MET practices are significantly perceived to be satisfactory for acquiring non-technical competencies, such as being proficient in the English language, but are less satisfactory for instilling technical competencies such as of electrical and electronics, knowledge regarding seaworthiness of ships, as well as medical first aid. In addition, the findings highlight the perceived gaps in existing marine engineering education as well as the need for future studies focused on re-training the future maritime workforce that could potentially improve MET strategies and practices in the developing nations.
Research on Whipping and Jetting Combined Swimming Behavior of Solen strictus Gould
Authors: Dong Zhaohui , Bi Shusheng , Liu Tian
Published: October 2021

Solen strictus Gould are mainly cave dwellers, using their axe feet to dig caves. The Solen strictus Gould also exhibit escape-swimming behavior when food, environment, and so on changes. In this paper, Solen strictus Gould were captured on a high-speed camera as they escaped, and it was found that the Solen strictus Gould whipped while the water spout between the axe foot and the mantle sprayed water outwards. The combined propulsion of these two methods allows the Solen strictus Gould to advance rapidly in a short time. It is calculated that the swimming speed of razor clams is positively correlated with their size and that the adult Solen strictus Gould can travel up to 12 times their body length per second. This study enriches the data on the behavioral characteristics of Solen strictus Gould and is of interest regarding Solen strictus Gould in artificial farming, marine fishing, offshore ecological restoration, and underwater bionic robot development.
Erosive Degradation Study of Concrete Augmented by Mussel Shells for Marine Construction
Authors: Camba Carolina , Mier José Luis , Carral Luis , Lamas María Isabel , Álvarez José Carlos , Díaz-Díaz Ana-María , Tarrío-Saavedra Javier
Published: October 2021

This work proposes a green material for artificial reefs to be placed in Galicia (northwest Spain) taking into account the principles of circular economy and sustainability of the ecosystem. New concrete formulations for marine applications, based on cement and/or sand replacement by mussel shells, are analyzed in terms of resistance to abrasion. The interest lies in the importance of the canning industry of Galicia, which generates important quantities of shell residues with negative environmental consequences. Currently, the tests to determine the abrasion erosion resistance of concrete on hydraulic structures involve large and complex devices. According to this, an experimental test has been proposed to estimate and compare the wear resistance of these concretes and, consequently, to analyze the environmental performance of these structures. First, a numerical analysis validated with experimental data was conducted to design the test. Subsequently, experimental tests were performed using a slurry tank in which samples with conventional cement and sand were partially replaced by mussel shell. The abrasive erosion effect of concrete components was analyzed by monitoring the mass loss. It shows an asymptotic trend with respect to time that has been modeled by Generalized Additive Model (GAM) and nonlinear regression models. The results were compared to concrete containing only conventional cement and sand. Replacing sand and/or cement by different proportions of mussel shells has not significantly reduced the resistance of concrete against erosive degradation, except for the case where a high amount of sand (20 wt.%) is replaced. Its resistance against the erosive abrasion is increased, losing between 0.1072 and 0.0310 wt.% lower than common concrete. In all the remaining cases (replacements of the 5–10 wt.% of sand and cement), the effect of mussel replacement on erosive degradation is not significant. These results encourage the use of mussel shells in the composition of concrete, taking into account that we obtain the same degradation properties, even more so considering an important residue in the canning industry (and part of the seabed) that can be valorized.
Genotoxicity of Polystyrene (PS) Microspheres in Short-Term Exposure to Gametes of the Sand Dollar Scaphechinus mirabilis (Agassiz, 1864) (Echinodermata, Echinoidea)
Authors: Mazur Andrey Alexandrovich , Chelomin Viktor Pavlovich , Zhuravel Elena Vladimirovna , Kukla Sergey Petrovich , Slobodskova Valentina Vladimirovna , Dovzhenko Nadezda Vladimirovna
Published: October 2021

Microplastic pollution appears to be one of the major environmental problems in the world today, and researchers have been paying special attention to the study of the impact of microplastics on biota. In this article, we studied the short-term effects of polystyrene micro-spheres on genome integrity using the gametes of the Scaphechinus mirabilis sand dollar with the comet assay method. This highly sensitive method allowed us to identify the level of genome damage in both gametes before and after short-term exposure to PS microparticles. It was shown that primary polystyrene microspheres at concentrations of 104, 105, and 106 particles/L had a genotoxic effect during short-term exposure to the sperm of the sand dollar S. mirabilis, which was expressed as a significant increase in sperm DNA damage. The highest percentage of DNA damage (more than 20%) was detected in spermatozoa exposed for 1 h in water containing 105 microspheres of plastic per 1 L. Additionally, at all concentrations of microplastic studied in the experiment, the genetic damage index (GDI) values in spermatozoa exceeded the control level. However, regardless of the level of DNA damage, spermatozoa retained the ability to fertilise eggs with up to 97% efficiency. We must acknowledge that the genotoxic property of microplastic against sperm to some extent predicts the development of long-term adverse effects of environmental significance.
Estimation of Propagation Speed and Direction of Nonlinear Internal Waves from Underway and Moored Measurements
Authors: Lee Seung-Woo , Nam Sunghyun
Published: October 2021

Propagation speed and direction of nonlinear internal waves (NLIWs) are important parameters for understanding the generation and propagation of waves, and ultimately clarifying regional ocean circulation. However, these parameters cannot be directly measured from in-situ instruments, but can only be estimated from post-processing in situ data. Herein, we present two methods and an optimal approach to estimate the propagation speed and direction of waves using underway and moored observations. The Doppler shift method estimates these parameters from apparent observations concerning a moving ship using the Doppler shift induced by the changing relative distance of the NLIWs from the moving ship. The time lag method estimates the parameters using the distance between two locations of the NLIW observed at different times and the time lag. To optimize the speed and direction of NLIWs, the difference in the propagation direction independently estimated by the two methods needs to be minimized concerning the optimal propagation speed to yield the optimal propagation direction. The methods were applied to two cases observed in the northern East China Sea in May 2015 and August 2018. This study has practical significance for better estimating the propagation speed and direction of NILWs particularly over a broad continental shelf.
Structure–Activity Relationship of Phytotoxic Natural 10-Membered Lactones and Their Semisynthetic Derivatives
Authors: Dalinova Anna , Fedorov Anatoly , Dubovik Vsevolod , Voitsekhovskaja Olga , Tyutereva Elena , Smirnov Sergey , Kochura Dmitry , Chisty Leonid , Senderskiy Igor , Berestetskiy Alexander
Published: October 2021

Ten-membered lactones (nonenolides) demonstrate phytotoxic, antimicrobial, and fungicidal activity promising for the development of natural product-derived pesticides. The fungus Stagonospora cirsii is able to produce phytotoxic stagonolides A (1), J (2), K (3) and herbarumin I (4) with high yield. The aim of this study was to create a set of structurally related nonenolides and to reveal the structural features that affect their biological activity. Stagonolide A (1) and C-7 oxidized stagonolide K (11) showed the highest phytotoxicity in leaf puncture assay and agar seedlings assay. The oxidation of C-7 hydroxyl group (as in 1, acetylstagonolide A (10) and (11) led to the manifestation of toxicity to microalgae, Bacillus subtilis and Sf9 cells regardless of the configuration of C-9 propyl chains (R in 1 and 10, S in 11). C-7 non-oxidized nonenolides displayed none or little non-target activity. Notably, 7S compounds were more phytotoxic than their 7R analogues. Due to the high inhibitory activity against seedling growth and the lack of side toxicity, mono- and bis(acetyl)- derivatives of herbarumin I were shown to be potent for the development of pre-emergent herbicides. The identified structural features can be used for the rational design of new herbicides.
Cell Wall Integrity Pathway Involved in Morphogenesis, Virulence and Antifungal Susceptibility in Cryptococcus neoformans
Authors: de Oliveira Haroldo Cesar , Rossi Suelen Andreia , García-Barbazán Irene , Zaragoza Óscar , Trevijano-Contador Nuria
Published: October 2021

Due to its location, the fungal cell wall is the compartment that allows the interaction with the environment and/or the host, playing an important role during infection as well as in different biological functions such as cell morphology, cell permeability and protection against stress. All these processes involve the activation of signaling pathways within the cell. The cell wall integrity (CWI) pathway is the main route responsible for maintaining the functionality and proper structure of the cell wall. This pathway is highly conserved in the fungal kingdom and has been extensively characterized in Saccharomyces cerevisiae. However, there are still many unknown aspects of this pathway in the pathogenic fungi, such as Cryptococcus neoformans. This yeast is of particular interest because it is found in the environment, but can also behave as pathogen in multiple organisms, including vertebrates and invertebrates, so it has to adapt to multiple factors to survive in multiple niches. In this review, we summarize the components of the CWI pathway in C. neoformans as well as its involvement in different aspects such as virulence factors, morphological changes, and its role as target for antifungal therapies among others.
Aspergillus Goes Viral: Ecological Insights from the Geographical Distribution of the Mycovirome within an Aspergillus flavus Population and Its Possible Correlation with Aflatoxin Biosynthesis
Authors: Degola Francesca , Spadola Giorgio , Forgia Marco , Turina Massimo , Dramis Lucia , Chitarra Walter , Nerva Luca
Published: October 2021

Microbial multi-level interactions are essential to control the success of spreading and survival of most microbes in natural environments. Phytopathogenic mycotoxigenic fungal species, such as Aspergillus flavus, represent an important issue in food safety. Usually, non-toxigenic strains are exploited for biocontrol strategies to mitigate infections by toxigenic strains. To comprehend all the biological variables involved in the aflatoxin biosynthesis, and to possibly evaluate the interplay between A. flavus toxigenic and non-toxigenic strains during intraspecific biocompetition, the “virological” perspective should be considered. For these reasons, investigations on mycoviruses associated to A. flavus populations inhabiting specific agroecosystems are highly desirable. Here, we provide the first accurate characterization of the novel mycovirome identified within an A. flavus wild population colonizing the maize fields of northern Italy: a selection of A. flavus strains was biologically characterized and subjected to RNAseq analysis, revealing new mycoviruses and a peculiar geographic pattern distribution in addition to a 20% rate of infection. More interestingly, a negative correlation between viral infection and aflatoxin production was found. Results significantly expanded the limited existent data about mycoviruses in wild A. flavus, opening new and intriguing hypotheses about the ecological significance of mycoviruses.
Direct and Indirect Effects of COVID-19 in Frail Elderly: Interventions and Recommendations
Authors: Pizano-Escalante María Guadalupe , Anaya-Esparza Luis Miguel , Nuño Karla , Rodríguez-Romero José de Jesús , Gonzalez-Torres Sughey , López-de la Mora David A. , Villagrán Zuamí
Published: October 2021

Frailty is a state of vulnerability to stressors because of a decreased physiological reserve, resulting in poor health outcomes. This state is related to chronic conditions, many of which are risk factors for outcomes in elderly patients having SARS-COV-2. This review aims to describe frailty as a physiological vulnerability agent during the COVID-19 pandemic in elderly patients, summarizing the direct and indirect effects caused by the SARS-COV-2 infection and its prognosis in frail individuals, as well as the interventions and recommendations to reduce their effects. Cohort studies have shown that patients with a Clinical Frailty Scale higher than five have a higher risk of mortality and use of mechanical ventilation after COVID-19; nonetheless, other scales have also associated frailty with longer hospital stays and more severe forms of the disease. Additionally, the indirect effects caused by the pandemic have a negative impact on the health status of older people. Due to the above, a holistic intervention is proposed based on a comprehensive geriatric assessment for frail patients (preventive or post-infection) with emphasis on physical activity and nutritional recommendations, which could be a potential preventive intervention in viral infections by COVID-19.
Plasma Anion Gap and Risk of In-Hospital Mortality in Patients with Acute Ischemic Stroke: Analysis from the MIMIC-IV Database
Authors: Jhou Hong-Jie , Chen Po-Huang , Yang Li-Yu , Chang Shu-Hao , Lee Cho-Hao
Published: October 2021

We aimed to investigate the association between the plasma anion gap (AG) and in-hospital mortality among patients with acute ischemic stroke (AIS). In total, 1236 AIS patients were enrolled using the Medical Information Mart for Intensive Care Database IV. Primary outcome was in-hospital mortality. The patients were divided into four groups according to AG category. The mean age and Charlson comorbidity index increased as the AG category increased. The fourth AG category was most related to the in-hospital mortality (hazards ratio (HR), 95% confidence interval (CI): 2.77, 1.60–4.71), even after adjusting for possible confounding variables (Model 1: HR, 95% CI: 3.37, 1.81–6.09; Model 2: HR, 95% CI: 3.57, 1.91–6.69). Moreover, intensive care unit mortality (p = 0.008) was higher in the highest AG category, but the intracranial hemorrhage (p = 0.071) did not associate with the plasma AG. The plasma AG had a satisfactory predictive ability for in-hospital mortality among AIS patients (areas under the receiver operating characteristic curve: 0.631). The plasma AG is an independent risk factor that can satisfactorily predict the in-hospital mortality among AIS patients.
Use of Mobile Grocery Shopping Application: Motivation and Decision-Making Process among South Korean Consumers
Authors: Kim Hyungjoon
Published: October 2021

With the revitalization of the online grocery trading market, many consumers are using mobile applications to purchase groceries. Although past studies were conducted on online grocery purchases, few measured mobile app users in a conceptual model that combines both motivational needs and behavioral components. Grounded in the uses and gratifications theory and the theory of planned behavior, this study investigated utilitarian motives, hedonic motives, experiential motives, attitudes, subjective norms, perceived behavioral control, purchase intention, and purchase behavior among mobile grocery app users in South Korea. As an additional analysis, a comparison between users and non-users of mobile grocery apps was implemented. The results showed that the utilitarian motives of grocery app users significantly influenced attitudes, attitudes and subjective norms influenced user intention, and user intention influenced grocery purchase behavior. Users showed statistically higher utilitarian motives, hedonic motives, and attitudes than non-users. The results suggest that South Korean consumers hold positive attitudes toward mobile grocery shopping and that the opinions of others may influence the decision to use the services. Mobile groceries in South Korea may have the potential for continued growth if individuals’ perceived control of the service improves. Implications and suggestions for future research are discussed.
Shoreline Evolution and Environmental Changes at the NW Area of the Gulf of Gela (Sicily, Italy)
Authors: Borzì Laura , Anfuso Giorgio , Manno Giorgio , Distefano Salvatore , Urso Salvatore , Chiarella Domenico , Di Stefano Agata
Published: October 2021

Coastal areas are among the most biologically productive, dynamic and valued ecosystems on Earth. They are subject to changes that greatly vary in scale, time and duration and to additional pressures resulting from anthropogenic activities. The aim of this work was to investigate the shoreline evolution and the main environmental changes of the coastal stretch between the towns of Licata and Gela (in the Gulf of Gela, Sicily, Italy). The methodology used in this work included the analysis of: (i) shoreline changes over the long- and medium-term periods (1955–2019 and 1989–2019, respectively), (ii) dune system fragmentation and (iii) the impact of coastal structures (harbours and breakwaters) on coastal evolution. The shoreline change analysis mainly showed a negative trend both over the long- and medium-term periods, with a maximum retreat of 3.87 m/year detected over the medium-term period down-drift of the Licata harbour. However, a few kilometres eastward from the harbour, significant accretion was registered where a set of breakwaters was emplaced. The Shoreline Change Envelope (SCE) showed that the main depositional phenomena occurred during the decade between 1955 and 1966, whereas progressive and constant erosion was observed between 1966 and 1989 in response to the increasing coastal armouring.