bluuebunny/crossref_metadata_2025 huggingface dataset:
Responding to Climate and Environmental Change Impacts on Human Health via Integrated Surveillance in the Circumpolar North: A Systematic Realist Review
Authors: Sawatzky Alexandra , Cunsolo Ashlee , Jones-Bitton Andria , Middleton Jacqueline , Harper Sherilee L.
Published: November 2018

Environments are shifting rapidly in the Circumpolar Arctic and Subarctic regions as a result of climate change and other external stressors, and this has a substantial impact on the health of northern populations. Thus, there is a need for integrated surveillance systems designed to monitor the impacts of climate change on human health outcomes as part of broader adaptation strategies in these regions. This review aimed to identify, describe, and synthesize literature on integrated surveillance systems in Circumpolar Arctic and Subarctic regions, that are used for research or practice. Following a systematic realist review approach, relevant articles were identified using search strings developed for MEDLINE® and Web of Science™ databases, and screened by two independent reviewers. Articles that met the inclusion criteria were retained for descriptive quantitative analysis, as well as thematic qualitative analysis, using a realist lens. Of the 3431 articles retrieved in the database searches, 85 met the inclusion criteria and were analyzed. Thematic analysis identified components of integrated surveillance systems that were categorized into three main groups: structural, processual, and relational components. These components were linked to surveillance attributes and activities that supported the operations and management of integrated surveillance. This review advances understandings of the distinct contributions of integrated surveillance systems and data to discerning the nature of changes in climate and environmental conditions that affect population health outcomes and determinants in the Circumpolar North. Findings from this review can be used to inform the planning, design, and evaluation of integrated surveillance systems that support evidence-based public health research and practice in the context of increasing climate change and the need for adaptation.
Preface to “A Commemorative Issue in Honour of Professor Nick Hadjiliadis: Metal Complex Interactions with Nucleic Acids and/or DNA”
Authors: Banti Christina N. , Hadjikakou Sotiris K.
Published: November 2018

This Special Issue of the International Journal of Molecular Science comprises a comprehensive study on "Metal Complex Interactions with Nucleic Acids and/or DNA". [...]
Unambiguous Ex Situ and in Cell 2D 13C Solid-State NMR Characterization of Starch and Its Constituents
Authors: Poulhazan Alexandre , Arnold Alexandre A. , Warschawski Dror E. , Marcotte Isabelle
Published: November 2018

Starch is the most abundant energy storage molecule in plants and is an essential part of the human diet. This glucose polymer is composed of amorphous and crystalline domains in different forms (A and B types) with specific physicochemical properties that determine its bioavailability for an organism, as well as its value in the food industry. Using two-dimensional (2D) high resolution solid-state nuclear magnetic resonance (SS-NMR) on 13C-labelled starches that were obtained from Chlamydomonas reinhardtii microalgae, we established a complete and unambiguous assignment for starch and its constituents (amylopectin and amylose) in the two crystalline forms and in the amorphous state. We also assigned so far unreported non-reducing end groups and assessed starch chain length, crystallinity and amylose content. Starch was then characterized in situ, i.e., by 13C solid-state NMR of intact microalgal cells. Our in-cell methodology also enabled the identification of the effect of nitrogen starvation on starch metabolism. This work shows how solid-state NMR can enable the identification of starch structure, chemical modifications and biosynthesis in situ in intact microorganisms, eliminating time consuming and potentially altering purification steps.
α-Melanocyte-Stimulating Hormone Attenuates Neovascularization by Inducing Nitric Oxide Deficiency via MC-Rs/PKA/NF-κB Signaling
Authors: Weng Wen-Tsan , Wu Chieh-Shan , Wang Feng-Sheng , Wu Chang-Yi , Ma Yi-Ling , Chan Hoi-Hung , Wu Den-Chiung , Wu Jian-Ching , Chu Tian-Huei , Huang Shih-Chung , Tai Ming-Hong
Published: November 2018

α-melanocyte-stimulating hormone (α-MSH) has been characterized as a novel angiogenesis inhibitor. The homeostasis of nitric oxide (NO) plays an important role in neovascularization. However, it remains unclear whether α-MSH mitigates angiogenesis through modulation of NO and its signaling pathway. The present study elucidated the function and mechanism of NO signaling in α-MSH-induced angiogenesis inhibition using cultured human umbilical vein endothelial cells (HUVECs), rat aorta rings, and transgenic zebrafish. By Griess reagent assay, it was found α-MSH dose-dependently reduced the NO release in HUVECs. Immunoblotting and immunofluorescence analysis revealed α-MSH potently suppressed endothelial and inducible nitric oxide synthase (eNOS/iNOS) expression, which was accompanied with inhibition of nuclear factor kappa B (NF-κB) activities. Excessive supply of NO donor l-arginine reversed the α-MSH-induced angiogenesis inhibition in vitro and in vivo. By using antibody neutralization and RNA interference, it was delineated that melanocortin-1 receptor (MC1-R) and melanocortin-2 receptor (MC2-R) participated in α-MSH-induced inhibition of NO production and NF-κB/eNOS/iNOS signaling. This was supported by pharmaceutical inhibition of protein kinase A (PKA), the downstream effector of MC-Rs signaling, using H89 abolished the α-MSH-mediated suppression of NO release and eNOS/iNOS protein level. Therefore, α-MSH exerts anti-angiogenic function by perturbing NO bioavailability and eNOS/iNOS expression in endothelial cells.
The Role of NADPH Oxidases and Oxidative Stress in Neurodegenerative Disorders
Authors: Tarafdar Anuradha , Pula Giordano
Published: November 2018

For a number of years, nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOX) was synonymous with NOX2/gp91phox and was considered to be a peculiarity of professional phagocytic cells. Over the last decade, several more homologs have been identified and based on current research, the NOX family consists of NOX1, NOX2, NOX3, NOX4, NOX5, DUOX1 and DUOX2 enzymes. NOXs are electron transporting membrane proteins that are responsible for reactive oxygen species (ROS) generation—primarily superoxide anion (O2●−), although hydrogen peroxide (H2O2) can also be generated. Elevated ROS leads to oxidative stress (OS), which has been associated with a myriad of inflammatory and degenerative pathologies. Interestingly, OS is also the commonality in the pathophysiology of neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS). NOX enzymes are expressed in neurons, glial cells and cerebrovascular endothelial cells. NOX-mediated OS is identified as one of the main causes of cerebrovascular damage in neurodegenerative diseases. In this review, we will discuss recent developments in our understanding of the mechanisms linking NOX activity, OS and neurodegenerative diseases, with particular focus on the neurovascular component of these conditions. We conclude highlighting current challenges and future opportunities to combat age-related neurodegenerative disorders by targeting NOXs.
CTCF Expression is Essential for Somatic Cell Viability and Protection Against Cancer
Authors: Bailey Charles G , Metierre Cynthia , Feng Yue , Baidya Kinsha , Filippova Galina N , Loukinov Dmitri I , Lobanenkov Victor V , Semaan Crystal , Rasko John EJ
Published: November 2018

CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. Cancer genome sequencing reveals diverse acquired mutations in CTCF, which we have shown functions as a tumour suppressor gene. While CTCF is essential for embryonic development, little is known of its absolute requirement in somatic cells and the consequences of CTCF haploinsufficiency. We examined the consequences of CTCF depletion in immortalised human and mouse cells using shRNA knockdown and CRISPR/Cas9 genome editing as well as examined the growth and development of heterozygous Ctcf (Ctcf+/−) mice. We also analysed the impact of CTCF haploinsufficiency by examining gene expression changes in CTCF-altered endometrial carcinoma. Knockdown and CRISPR/Cas9-mediated editing of CTCF reduced the cellular growth and colony-forming ability of K562 cells. CTCF knockdown also induced cell cycle arrest and a pro-survival response to apoptotic insult. However, in p53 shRNA-immortalised Ctcf+/− MEFs we observed the opposite: increased cellular proliferation, colony formation, cell cycle progression, and decreased survival after apoptotic insult compared to wild-type MEFs. CRISPR/Cas9-mediated targeting in Ctcf+/− MEFs revealed a predominance of in-frame microdeletions in Ctcf in surviving clones, however protein expression could not be ablated. Examination of CTCF mutations in endometrial cancers showed locus-specific alterations in gene expression due to CTCF haploinsufficiency, in concert with downregulation of tumour suppressor genes and upregulation of estrogen-responsive genes. Depletion of CTCF expression imparts a dramatic negative effect on normal cell function. However, CTCF haploinsufficiency can have growth-promoting effects consistent with known cancer hallmarks in the presence of additional genetic hits. Our results confirm the absolute requirement for CTCF expression in somatic cells and provide definitive evidence of CTCF’s role as a haploinsufficient tumour suppressor gene. CTCF genetic alterations in endometrial cancer indicate that gene dysregulation is a likely consequence of CTCF loss, contributing to, but not solely driving cancer growth.
Thermogravitational Cycles: Theoretical Framework and Example of an Electric Thermogravitational Generator Based on Balloon Inflation/Deflation
Authors: Aouane Kamel , Sandre Olivier , Ford Ian J. , Elson Tim P. , Nightingale Chris
Published: November 2018

Several studies have involved a combination of heat and gravitational energy exchanges to create novel heat engines. A common theoretical framework is developed here to describe thermogravitational cycles which have the same efficiencies as the Carnot, Rankine, or Brayton cycles. Considering a working fluid enclosed in a balloon inside a column filled with a transporting fluid, a cycle is composed of four steps. Starting from the top of the column, the balloon goes down by gravity, receives heat from a hot source at the bottom, then rises and delivers heat to a cold source at the top. Unlike classic power cycles which need external work to operate the compressor, thermogravitational cycles can operate as a “pure power cycle” where no external work is needed to drive the cycle. To illustrate this concept, the prototype of a thermogravitational electrical generator is presented. It uses a hot source of average temperature near 57 °C and relies on the gravitational energy exchanges of an organic fluorinated fluid inside a balloon attached to a magnetic marble to produce an electromotive force of 50 mV peak to peak by the use of a linear alternator. This heat engine is well suited to be operated using renewable energy sources such as geothermal gradients or focused sunlight.
Anthropogenic and Natural Radiative Forcing: Positive Feedbacks
Authors: Pinault Jean-Louis
Published: November 2018

This article is based on recent work intended to estimate the impact of solar forcing mediated by long-period ocean Rossby waves that are resonantly forced—the ‘Gyral Rossby Waves’ (GRWs). Here, we deduce both the part of the anthropogenic and climate components within the instrumental surface temperature spatial patterns. The natural variations in temperature are estimated from a weighted sum of sea surface temperature anomalies in preselected areas of subtropical gyres representative of long-period GRWs. The temperature response to anthropogenic forcing is deduced by subtracting the climate component from the instrumental temperature. Depending on whether the inland regions are primarily impacted by latent or sensible heat fluxes from the oceans, positive feedbacks occur. This suggests that the lapse rate and the high troposphere cloud cover have a driving role in the amplification effect of anthropogenic climate forcing, while specifying the involved mechanisms.
Tribological Behavior of Lamellar Molybdenum Trioxide as a Lubricant Additive
Authors: Tang Wei , Liu Rui , Lu Xiangyong , Zhang Shaogang , Liu Songyong
Published: November 2018

In this study, the tribological behavior of lamellar MoO3 as a lubricant additive was investigated under different concentrations, particle sizes, normal loads, velocity, and temperature. The friction and wear tests were performed using a tribometer and with a reciprocating motion. The results indicate that the friction-reducing ability and antiwear property of the base oil can be improved effectively with the addition of lamellar MoO3. The 0.5 wt % and 0.1 wt % concentrations of MoO3 yield the best antifriction and antiwear effects, respectively. The maximum friction and wear reduction is 19.8% and 55.9%, compared with that of the base oil. It is also found the MoO3 additive can decrease the friction considerably under a high velocity and normal load, and increase the working temperature. The smaller the size of MoO3, the better the friction-reducing effect the lamellar MoO3 shows. The friction-reducing and antiwear mechanisms of lamellar MoO3 were discussed.
The Influence of PSA Pre-Anodization of AA2024 on PEO Coating Formation: Composition, Microstructure, Corrosion, and Wear Behaviors
Authors: Serdechnova Maria , Karpushenkov Sergey A. , Karpushenkava Larisa S. , Starykevich Maksim , Ferreira Mario G. S. , Hack Theodor , Iuzviuk Mariia H. , Zobkalo Igor A. , Blawert Carsten , Zheludkevich Mikhail L.
Published: November 2018

In the frame of the current work, it was shown that plasma electrolytic oxidation (PEO) treatment can be applied on top of phosphoric sulfuric acid (PSA) anodized aluminum alloy AA2024. Being hard and well-adherent to the substrate, PEO layers improve both corrosion and wear resistance of the material. To facilitate PEO formation and achieve a dense layer, the systematic analysis of PEO layer formation on the preliminary PSA anodized layer was performed in this work. The microstructure, morphology, and composition of formed PEO coatings were investigated using scanning electron microscopy (SEM), x-ray diffraction (XRD), and glow-discharge optical emission spectroscopy (GDOES). It was shown that under constant current treatment conditions, the PSA layer survived under the applied voltage of 350 V, whilst 400 V was an intermediate stage; and under 450 V, the PSA layer was fully converted after 5 min of the treatment. The comparison test with PEO formation on the bare material was performed. It was confirmed that during the “sparking” mode (400 V) of PEO formation, the PEO coatings, formed on PSA treated AA2024, were more wear resistant than the same PEO coatings on bare AA2024.
Soft-Material-Based Smart Insoles for a Gait Monitoring System
Authors: Wang Changwon , Kim Young , Min Se Dong
Published: November 2018

Spatiotemporal analysis of gait pattern is meaningful in diagnosing and prognosing foot and lower extremity musculoskeletal pathologies. Wearable smart sensors enable continuous real-time monitoring of gait, during daily life, without visiting clinics and the use of costly equipment. The purpose of this study was to develop a light-weight, durable, wireless, soft-material-based smart insole (SMSI) and examine its range of feasibility for real-time gait pattern analysis. A total of fifteen healthy adults (male: 10, female: 5, age 25.1 ± 2.64) were recruited for this study. Performance evaluation of the developed insole sensor was first executed by comparing the signal accuracy level between the SMSI and an F-scan. Gait data were simultaneously collected by two sensors for 3 min, on a treadmill, at a fixed speed. Each participant walked for four times, randomly, at the speed of 1.5 km/h (C1), 2.5 km/h (C2), 3.5 km/h (C3), and 4.5 km/h (C4). Step count from the two sensors resulted in 100% correlation in all four gait speed conditions (C1: 89 ± 7.4, C2: 113 ± 6.24, C3: 141 ± 9.74, and C4: 163 ± 7.38 steps). Stride-time was concurrently determined and R2 values showed a high correlation between the two sensors, in both feet (R2 ≥ 0.90, p < 0.05). Bilateral gait coordination analysis using phase coordination index (PCI) was performed to test clinical feasibility. PCI values of the SMSI resulted in 1.75 ± 0.80% (C1), 1.72 ± 0.81% (C2), 1.72 ± 0.79% (C3), and 1.73 ± 0.80% (C4), and those of the F-scan resulted in 1.66 ± 0.66%, 1.70 ± 0.66%, 1.67 ± 0.62%, and 1.70 ± 0.62%, respectively, showing the presence of a high correlation (R2 ≥ 0.94, p < 0.05). The insole developed in this study was found to have an equivalent performance to commercial sensors, and thus, can be used not only for future sensor-based monitoring device development studies but also in clinical setting for patient gait evaluations.
Synthesis and Optimization of Chitosan Ceramic-Supported Membranes in Pervaporation Ethanol Dehydration
Authors: Nikbakht Fini Mahdi , Soroush Sepideh , Montazer-Rahmati Mohammad
Published: November 2018

In the present work, ceramic-supported chitosan hybrid membranes were prepared for the pervaporation dehydration of ethanol. Mullite and combined mullite-alumina (50% alumina content) tubular low-cost ceramic supports were fabricated, and their influence on membrane performance was compared to a commercial α-alumina support. The membrane preparation parameters were different ceramic supports and the concentration of chitosan solution (varying from 2 wt.% to 4 wt.%). The supports and hybrid membranes were characterized by field emission scanning electron microscopy (FE-SEM) and contact angle measurements. The results show, with increasing chitosan concentration, the permeability decreases, and selectivity increases. It was also found that the separation factor decreases with increasing feed temperature and feed water content, while the permeation flux increases. The membrane that was coated on α-alumina support with a 3 wt.% chitosan concentration exhibited the best pervaporation performance, leading to a permeation flux and separation factor of 352 g·m−2·h−1 and 200 for 90 wt.% ethanol in feed at 60 °C, respectively.
Untargeted Metabolic Profiling Cell-Based Approach of Pulmonary Artery Smooth Muscle Cells in Response to High Glucose and the Effect of the Antioxidant Vitamins D and E
Authors: Alamri Abdulwahab , Burzangi Abdulhadi S. , Coats Paul , Watson David G.
Published: November 2018

Pulmonary arterial hypertension (PAH) is a multi-factorial disease characterized by the hyperproliferation of pulmonary artery smooth muscle cells (PASMCs). Excessive reactive oxygen species (ROS) formation resulted in alterations of the structure and function of pulmonary arterial walls, leading to right ventricular failure and death. Diabetes mellitus has not yet been implicated in pulmonary hypertension. However, recently, variable studies have shown that diabetes is correlated with pulmonary hypertension pathobiology, which could participate in the modification of pulmonary artery muscles. The metabolomic changes in PASMCs were studied in response to 25 mM of D-glucose (high glucose, or HG) in order to establish a diabetic-like condition in an in vitro setting, and compared to five mM of D-glucose (normal glucose, or LG). The effect of co-culturing these cells with an ideal blood serum concentration of cholecalciferol-D3 and tocopherol was also examined. The current study aimed to examine the role of hyperglycemia in pulmonary arterial hypertension by the quantification and detection of the metabolomic alteration of smooth muscle cells in high-glucose conditions. Untargeted metabolomics was carried out using hydrophilic interaction liquid chromatography and high-resolution mass spectrometry. Cell proliferation was assessed by cell viability and the [3H] thymidine incorporation assay, and the redox state within the cells was examined by measuring reactive oxygen species (ROS) generation. The results demonstrated that PASMCs in high glucose (HG) grew, proliferated faster, and generated higher levels of superoxide anion (O2·−) and hydrogen peroxide (H2O2). The metabolomics of cells cultured in HG showed that the carbohydrate pathway, especially that of the upper glycolytic pathway metabolites, was influenced by the activation of the oxidation pathway: the pentose phosphate pathway (PPP). The amount of amino acids such as aspartate and glutathione reduced via HG, while glutathione disulfide, N6-Acetyl-L-lysine, glutamate, and 5-aminopentanoate increased. Lipids either as fatty acids or glycerophospholipids were downregulated in most of the metabolites, with the exception of docosatetraenoic acid and PG (16:0/16:1(9Z)). Purine and pyrimidine were influenced by hyperglycaemia following PPP oxidation. The results in addition showed that cells exposed to 25 mM of glucose were oxidatively stressed comparing to those cultured in five mM of glucose. Cholecalciferol (D3, or vitamin D) and tocopherol (vitamin E) were shown to restore the redox status of many metabolic pathways.
Recent Progress in Flexible Organic Thermoelectrics
Authors: Culebras Mario , Choi Kyungwho , Cho Chungyeon
Published: November 2018

Environmental energy issues caused by the burning of fossil fuel such as coal, and petroleum, and the limited resources along with the increasing world population pose a world-wide challenge. Alternative energy sources including solar energy, wind energy, and biomass energy, have been suggested as practical and affordable solutions to future energy needs. Among energy conversion technologies, thermoelectric (TE) materials are considered one of the most potential candidates to play a crucial role in addressing today’s global energy issues. TE materials can convert waste heat such as the sun, automotive exhaust, and industrial processes to a useful electrical voltage with no moving parts, no hazardous working chemical-fluids, low maintenance costs, and high reliability. These advantages of TE conversion provide solutions to solve the energy crisis. Here, we provide a comprehensive review of the recent progress on organic TE materials, focused on polymers and their corresponding organic composites incorporated with carbon nanofillers (including graphene and carbon nanotubes). Various strategies to enhance the TE properties, such as electrical conductivity and the Seebeck coefficient, in polymers and polymer composites will be highlighted. Then, a discussion on polymer composite based TE devices is summarized. Finally, brief conclusions and outlooks for future research efforts are presented.
Zircon Xenocrysts from Cenozoic Alkaline Basalts of the Ratanakiri Volcanic Province (Cambodia), Southeast Asia—Trace Element Geochemistry, O-Hf Isotopic Composition, U-Pb and (U-Th)/He Geochronology—Revelations into the Underlying Lithospheric Mantle
Authors: Piilonen Paula C. , Sutherland F. Lin , Danišík Martin , Poirier Glenn , Valley John W. , Rowe Ralph
Published: November 2018

Zircon xenocrysts from alkali basalts in Ratanakiri Province, Cambodia represent a unique low-Hf zircon within a 12,000 km long Indo-Pacific megacryst zone. Colorless, yellow, brown, and red crystals ({100}, {101}, subordinate {211}, {1103}), with hopper growth and corrosion features range up to 20 cm in size. Zircon chemistry indicates juvenile, Zr-saturated, mantle-derived alkaline melt (Hf 0.6–0.7 wt %, Y <0.2 wt %, U + Th + REE (Rare-Earth Elements) < 600 ppm, Zr/Hf 66–92, Eu/Eu*N ~1, positive Ce/Ce*N, HREE (Heavy REE) enrichment). Incompatible element depletion with increasing Yb/SmN from core to rim at ~ constant Hf suggests single stage growth. Ti-in-zircon temperatures (~570–740 °C) are lower than predicted by crystal morphology (800–900 °C) and decrease from core to rim (ΔT = 10–50 °C). The δ18O values (4.88 to 5.01‰ VSMOW (Vienna Standard Mean Ocean Water)) are relatively low for xenocrysts from the zircon Indo-Pacific zone (ZIP). The 176Hf/177Hf values (+ εHf 4.5–10.2) give TDepleted Mantle model source ages of 260–462 Ma and TCrustal ages of 391–754 Ma. The source magmas reflect variably depleted lithospheric mantle with little supracrustal input. Zircon U-Pb (0.88–1.56 Ma) and (U-Th)/He (0.86–1.02 Ma) ages are older than host basalt ages (~0.7 Ma), which suggests limited residence before transport. Zircon genesis suggests Zr-saturated, Al-undersaturated, carbonatitic-influenced, low-degree partial melting (<1%) of peridotitic mantle at ~60 km beneath the Indochina terrane.
Investigation of the Effects of Molecular Parameters on the Hemostatic Properties of Chitosan
Authors: Hu Zhang , Lu Sitong , Cheng Yu , Kong Songzhi , Li Sidong , Li Chengpeng , Yang Lei
Published: November 2018

Hemorrhea is one of the major problems in war, trauma care, and surgical operation that threaten the life of the injured and patients. As a novel polymeric hemostatic agent, biodegradable chitosan can stop bleeding through a variety of approaches. In this paper, chitosan with various molecular parameters was prepared from chitin as raw material through deacetylation, oxidative degradation, hydrophilic modification, and salt formation reactions. The influence of different polymer parameters on the hemostatic effects of chitosan was investigated by in vitro coagulation time and dynamic coagulation assay. The results showed that when the molecular weights were high (105–106) and approximate, the coagulation effect of chitosan improved with a decrease of the deacetylation degree and achieved a prominent level in a moderate degree of deacetylation (68.36%). With the same degree of deacetylation, the higher the molecular weight of chitosan, the better the procoagulant effect. The substituent derivatives and acid salts of chitosan showed significant procoagulant effects, especially the acid salts of chitosan. In addition, the hemostasis mechanism of chitosan with various parameters was preliminarily explored by analyzing the plasma recalcification time (PRT). The efforts in this paper laid a basis for further study of the structure–activity relationship and the mechanism of chitosan hemostasis.
The Prebiotic Activity of Simulated Gastric and Intestinal Digesta of Polysaccharides from the Hericium erinaceus
Authors: Yang Yang , Zhao Changhui , Diao Mengxue , Zhong Shuning , Sun Maocheng , Sun Bo , Ye Haiqing , Zhang Tiehua
Published: November 2018

Hericium erinaceus (HE) is a well-known edible and medicinal fungus widely grown in Asian countries. Polysaccharides from the Hericium erinaceus (HEP) are major biological macromolecules. It has been reported that HEP has multiple biological activities, such as antioxidant activity, immunomodulatory effects, anti-inflammatory effect, anti-chronic gastritis activity, and so on. In the current study, we investigated the biological property of HEP during gastrointestinal digestion. The results indicated that both simulated gastric and small intestinal digesta of HEP has better stimulation of probiotics growth than HEP alone, especially for Lactobacillus plantarum BG112. The prebiotic activity was the strongest when HEP was treated by simulated gastric juice for 2 h and by simulated small intestinal juice for 4 h. The molecular weight (Mw) of HEP decreased from 1.68 × 106 Da and 2.32 × 104 Da to 529.3 ± 7.2 Da, as digestion time increased. Meanwhile, the reducing sugar content was significantly increased from 0.610 ± 0.007 to 22.698 ± 0.752 mg/ml, suggesting that the decrease of Mw was likely due to the breakdown of glycosidic bonds. Considerable mannose and galactopyranose were released throughout the gastrointestinal digestion period, indicating that the gastrointestinal digestion resulted in production of free monosaccharides. After fermentation of L. plantarum BG112, the Mw of HEP was decreased and short chain fatty acids (SCFAs) including acetic acid, isovaleric acid, lactic acid, and butyric acid were produced. We speculated that the release of free monosaccharides during gastrointestinal digestion and utilization of HEP, by the probiotics, contributed to the prebiotic activity of HEP’s gastric and intestinal digesta. These results unveiled some mechanisms on the close relationship between the structure and bioactivity of polysaccharides, during digestion.
Leishmanicidal Activity of Withanolides from Aureliana fasciculata var. fasciculata
Authors: Lima Simone , Pacheco Juliana , Marques André , Veltri Eduardo , Almeida-Lafetá Rita , Figueiredo Maria , Kaplan Maria , Torres-Santos Eduardo
Published: November 2018

Leishmaniasis is the generic denomination to the neglected diseases caused by more than 20 species of protozoa belonging to the genus Leishmania. The toxic and parenteral-delivered pentavalent antimonials remain to be the first-line treatment. However, all the current used drugs have restrictions. The species Aureliana fasciculata (Vell.) Sendtner var. fasciculata is a native Brazilian species parsimoniously studied on a chemical point of view. In this study, the antileishmanial activity of A. fasciculata was evaluated. Among the evaluated samples of the leaves, the dichloromethane partition (AFfDi) showed the more pronounced activity, with IC50 1.85 µg/ml against promastigotes of L. amazonensis. From AFfDi, two active withanolides were isolated, the Aurelianolides A and B, with IC50 7.61 μM and 7.94 μM, respectively. The withanolides also proved to be active against the clinically important form, the intracellular amastigote, with IC50 2.25 μM and 6.43 μM for Aurelianolides A and B, respectively. Furthermore, withanolides showed results for in silico parameters of absorption, distribution, metabolism, excretion, and toxicity (ADMET) similar to miltefosine, the reference drug, and were predicted as good oral drugs, with the advantage of not being hepatotoxic. These results suggest that these compounds can be useful as scaffolds for planning drug design.
Adsorption of Sb (III) on Oxidized Exfoliated Graphite Nanoplatelets
Authors: Capra Luiza , Manolache Mihaela , Ion Ion , Stoica Rusandica , Stinga Gabriela , Doncea Sanda Maria , Alexandrescu Elvira , Somoghi Raluca , Calin Marian Romeo , Radulescu Ileana , Ivan Georgeta Ramona , Deaconu Marian , Ion Alina Catrinel
Published: November 2018

In this work, Sb (III) adsorption on oxidized exfoliated graphite nanoplatelets (ox-xGnP) was evaluated for the first time, to the best of our knowledge. The ox-xGnP were characterized by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), Brunauer–Emmet–Teller (BET) analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM) equipped with energy-dispersive X-ray spectroscopy (EDX), and Zeta potential analysis. The adsorption parameters, such as pH and contact time, were optimized, and the best adsorption capacity obtained was 8.91 mg g−1 at pH = 7.0, 1.0 mg ox-xGnP/100 mL solution, T = 293 K, 1.0 mg L−1, Sb (III), 25 min contact time. The best correlation of the kinetic data was described by a pseudo-first-order kinetic model, with R2 = 0.999. The adsorption isotherms of Sb (III) onto ox-xGnP were best described by the Langmuir isotherm model. The thermodynamic parameters showed that the adsorption process was exothermic and spontaneous.
Correction: Che Idris, C.A., et al. Effect of Consumption Heated Oils with or without Dietary Cholesterol on the Development of Atherosclerosis. Nutrients 2018, 10, 1527
Authors: Che Idris Che , Sundram Kalyana , Abdull Razis Ahmad
Published: November 2018

The authors wish to make the following change to their paper (Che Idris et al. [...]
Functional Polymer Nanocarriers for Photodynamic Therapy
Authors: Li Tuanwei , Yan Lifeng
Published: November 2018

Photodynamic therapy (PDT) is an appealing therapeutic modality in management of some solid tumors and other diseases for its minimal invasion and non-systemic toxicity. However, the hydrophobicity and non-selectivity of the photosensitizers, inherent serious hypoxia of tumor tissues and limited penetration depth of light restrict PDT further applications in clinic. Functional polymer nanoparticles can be used as a nanocarrier for accurate PDT. Here, we elucidate the mechanism and application of PDT in cancer treatments, and then review some strategies to administer the biodistribution and activation of photosensitizers (PSs) to ameliorate or utilize the tumor hypoxic microenvironment to enhance the photodynamic therapy effect.
Empirical Assessment Tool for Bathymetry, Flow Velocity and Salinity in Estuaries Based on Tidal Amplitude and Remotely-Sensed Imagery
Authors: Leuven Jasper R. F. W. , Verhoeve Steye L. , Van Dijk Wout M. , Selaković Sanja , Kleinhans Maarten G.
Published: November 2018

Hydromorphological data for many estuaries worldwide is scarce and usually limited to offshore tidal amplitude and remotely-sensed imagery. In many projects, information about morphology and intertidal area is needed to assess the effects of human interventions and rising sea-level on the natural depth distribution and on changing habitats. Habitat area depends on the spatial pattern of intertidal area, inundation time, peak flow velocities and salinity. While numerical models can reproduce these spatial patterns fairly well, their data need and computational costs are high and for each case a new model must be developed. Here, we present a Python tool that includes a comprehensive set of relations that predicts the hydrodynamics, bed elevation and the patterns of channels and bars in mere seconds. Predictions are based on a combination of empirical relations derived from natural estuaries, including a novel predictor for cross-sectional depth distributions, which is dependent on the along-channel width profile. Flow velocity, an important habitat characteristic, is calculated with a new correlation between depth below high water level and peak tidal flow velocity, which was based on spatial numerical modelling. Salinity is calculated from estuarine geometry and flow conditions. The tool only requires an along-channel width profile and tidal amplitude, making it useful for quick assessments, for example of potential habitat in ecology, when only remotely-sensed imagery is available.
Residuals of Tropospheric Delays from GNSS Data and Ray-Tracing as a Potential Indicator of Rain and Clouds
Authors: Hordyniec Paweł , Kapłon Jan , Rohm Witold , Kryza Maciej
Published: November 2018

The Global Navigation Satellite System (GNSS) is commonly recognized by its all-weather capability. However, observations depend on atmospheric conditions which requires the induced tropospheric delay to be estimated as an unknown parameter. In the following study, we investigate the impact of intense weather events on GNSS estimates. GNSS slant total delays (STD) in Precise Point Positioning technique (PPP) strategy were calculated for stations in southwest Poland in a 56 days period covering several heavy precipitation cases. The corresponding delays retrieved from Weather Research and Forecasting (WRF) model by a ray-tracing technique considered only gaseous parts of the atmosphere. The discrepancies are correlated with rain rates and cloud type products from remote sensing platforms. Positive correlation is found as well as GNSS estimates tend to be systematically larger than modeled delays. Mean differences mapped to the zenith direction are showed to vary between 10 mm and 30 mm. The magnitude of discrepancies follows the intensity of phenomena, especially for severe weather events. Results suggest that effects induced by commonly neglected liquid and solid water terms in the troposphere modeling should be considered in precise GNSS applications for the atmosphere monitoring. The state-of-art functional model applied in GNSS processing strategies shows certain deficits. Estimated tropospheric delays with gradients and post-fit residuals could be replaced by a loosely constrained solution without loss of quality.
The Use of Unmanned Aerial Systems to Map Intertidal Sediment
Authors: Fairley Iain , Mendzil Anouska , Togneri Michael , Reeve Dominic E.
Published: November 2018

This paper describes a new methodology to map intertidal sediment using a commercially available unmanned aerial system (UAS). A fixed-wing UAS was flown with both thermal and multispectral cameras over three study sites comprising of sandy and muddy areas. Thermal signatures of sediment type were not observable in the recorded data and therefore only the multispectral results were used in the sediment classification. The multispectral camera consisted of a Red–Green–Blue (RGB) camera and four multispectral sensors covering the green, red, red edge and near-infrared bands. Statistically significant correlations (>99%) were noted between the multispectral reflectance and both moisture content and median grain size. The best correlation against median grain size was found with the near-infrared band. Three classification methodologies were tested to split the intertidal area into sand and mud: k-means clustering, artificial neural networks, and the random forest approach. Classification methodologies were tested with nine input subsets of the available data channels, including transforming the RGB colorspace to the Hue–Saturation–Value (HSV) colorspace. The classification approach that gave the best performance, based on the j-index, was when an artificial neural network was utilized with near-infrared reflectance and HSV color as input data. Classification performance ranged from good to excellent, with values of Youden’s j-index ranging from 0.6 to 0.97 depending on flight date and site.
An Overall Assessment of JPSS-2 VIIRS Radiometric Performance Based on Pre-Launch Testing
Authors: Oudrari Hassan , McIntire Jeff , Xiong Xiaoxiong , Butler James , Ji Qiang , Schwarting Thomas , Angal Amit
Published: November 2018

The Visible Infrared Imaging Radiometer Suite (VIIRS) on-board the second Joint Polar Satellite System (JPSS) completed its sensor level testing in February 2018. The JPSS-2 (J2) mission is scheduled to launch in 2022 and will be very similar to its two predecessor missions, the Suomi National Polar-orbiting Partnership (SNPP) mission, launched on 28 October 2011 and JPSS-1 (renamed NOAA-20) launched on 18 November 2017. VIIRS instrument has 22 spectral bands covering the spectrum between 0.4 and 12.6 μm: 14 reflective solar bands (RSB), 7 thermal emissive bands (TEB) and one day-night band (DNB). It is a cross-track scanning radiometer capable of providing global measurements through observations at two spatial resolutions, 375 m and 750 m at nadir for the imaging bands and moderate bands, respectively. This paper will provide an overview of J2 VIIRS characterization methodologies and calibration performance during the pre-launch testing phases performed by the National Aeronautics and Space Administration (NASA) VIIRS Characterization Support Team (VCST) to evaluate the at-launch baseline radiometric performance and generate the parameters needed to populate the sensor data record (SDR) Look-Up-Tables (LUTs). Our analysis results confirmed the good performance of J2 VIIRS, in general as good as previous VIIRS instruments and all non-compliances are expected to have low impact on data quality. Key sensor performance metrics include the signal to noise ratio (SNR), radiance dynamic range, reflective and emissive bands calibration performance, polarization sensitivity, spectral performance, response versus scan-angle (RVS) and scattered light response. A set of performance metrics generated during the pre-launch testing program will be compared to both the SNPP and JPSS-1 VIIRS sensors.
Physically-Based Retrieval of Canopy Equivalent Water Thickness Using Hyperspectral Data
Authors: Wocher Matthias , Berger Katja , Danner Martin , Mauser Wolfram , Hank Tobias
Published: November 2018

Quantitative equivalent water thickness on canopy level (EWTcanopy) is an important land surface variable and retrieving EWTcanopy from remote sensing has been targeted by many studies. However, the effect of radiative penetration into the canopy has not been fully understood. Therefore, in this study the Beer-Lambert law is applied to inversely determine water content information in the 930 to 1060 nm range of canopy reflectance from measured winter wheat and corn spectra collected in 2015, 2017, and 2018. The spectral model was calibrated using a look-up-table (LUT) of 50,000 PROSPECT spectra. Internal model validation was performed using two leaf optical properties datasets (LOPEX93 and ANGERS). Destructive in-situ measurements of water content were collected separately for leaves, stalks, and fruits. Correlation between measured and modelled water content was most promising for leaves and ears in case of wheat, reaching coefficients of determination (R2) up to 0.72 and relative RMSE (rRMSE) of 26% and in case of corn for the leaf fraction only (R2 = 0.86, rRMSE = 23%). These findings indicate that, depending on the crop type and its structure, different parts of the canopy are observed by optical sensors. The results from the Munich-North-Isar test sites indicated that plant compartment specific EWTcanopy allows us to deduce more information about the physical meaning of model results than from equivalent water thickness on leaf level (EWT) which is upscaled to canopy water content (CWC) by multiplication of the leaf area index (LAI). Therefore, it is suggested to collect EWTcanopy data and corresponding reflectance for different crop types over the entire growing cycle. Nevertheless, the calibrated model proved to be transferable in time and space and thus can be applied for fast and effective retrieval of EWTcanopy in the scope of future hyperspectral satellite missions.
Assessment of Sentinel-2 MSI Spectral Band Reflectances for Estimating Fractional Vegetation Cover
Authors: Wang Bing , Jia Kun , Liang Shunlin , Xie Xianhong , Wei Xiangqin , Zhao Xiang , Yao Yunjun , Zhang Xiaotong
Published: November 2018

Fractional vegetation cover (FVC) is an essential parameter for characterizing the land surface vegetation conditions and plays an important role in earth surface process simulations and global change studies. The Sentinel-2 missions carrying multi-spectral instrument (MSI) sensors with 13 multispectral bands are potentially useful for estimating FVC. However, the performance of these bands for FVC estimation is unclear. Therefore, the objective of this study was to assess the performance of Sentinel-2 MSI spectral band reflectances on FVC estimation. The samples, including the Sentinel-2 MSI canopy reflectances and corresponding FVC values, were simulated using the PROSPECT + SAIL radiative transfer model under different conditions, and random forest regression (RFR) method was then used to develop FVC estimation models and assess the performance of various band reflectances for FVC estimation. These models were finally evaluated using field survey data. The results indicate that the three most important bands of Sentinel-2 MSI data for FVC estimation are band 4 (Red), band 12 (SWIR2) and band 8a (NIR2). FVC estimation using these bands has a comparable accuracy (root mean square error (RMSE) = 0.085) with that using all bands (RMSE = 0.090). The results also demonstrate that band 12 had a better performance for FVC estimation than the green band (RMSE = 0.097). However, the newly added red-edge bands, with low scores in the RFR model, have little significance for improving FVC estimation accuracy compared with the Red, NIR2 and SWIR2 bands.
Integrating Spatial Continuous Wavelet Transform and Normalized Difference Vegetation Index to Map the Agro-Pastoral Transitional Zone in Northern China
Authors: Han Yinan , Peng Jian , Meersmans Jeroen , Liu Yanxu , Zhao Zhiqiang , Mao Qi
Published: November 2018

The agro-pastoral transitional zone (APTZ) in Northern China is one of the most important ecological barriers of the world. The commonly-used method to identify the spatial distribution of ATPZ is to apply a threshold rule on climatic or land use indicators. This approach is highly subjective, and the quantity standards vary among the studies. In this study, we adopted the spatial continuous wavelet transform (SCWT) technique to detect the spatial fluctuation in normalized difference vegetation index (NDVI) sequences, and as such identify the APTZ. To carry out this analysis, the Moderate Resolution Imaging Spectroradiometer (MODIS) NDVI 1-month data (MODND1M) covering the period 2006–2015 were used. Based on the spatial variation in NDVI, we identified two sub-regions within the APTZ. The temporal change of APTZ showed that although vegetation spatial pattern changed annually, certain areas appeared to be stable, while others showed higher sensitivity to environmental variance. Through correlation analysis between the dynamics of APTZ and precipitation, we found that the mean center of the APTZ moved toward the southeast during dry years and toward the northwest during humid years. By comparing the APTZ spatial pattern obtained in the present study with the outcome following the traditional approach based on mean annual precipitation data, it can be concluded that our study provides a reliable basis to advance the methodological framework to identify accurately transitional zones. The identification framework is of high importance to support decision-making in land use management in Northern China as well as other similar regions around the world.
A Collaborative UAV-WSN Network for Monitoring Large Areas
Authors: Popescu Dan , Dragana Cristian , Stoican Florin , Ichim Loretta , Stamatescu Grigore
Published: November 2018

Large-scale monitoring systems have seen rapid development in recent years. Wireless sensor networks (WSN), composed of thousands of sensing, computing and communication nodes, form the backbone of such systems. Integration with unmanned aerial vehicles (UAVs) leads to increased monitoring area and to better overall performance. This paper presents a hybrid UAV-WSN network which is self-configured to improve the acquisition of environmental data across large areas. A prime objective and novelty of the heterogeneous multi-agent scheme proposed here is the optimal generation of reference trajectories, parameterized after inter- and intra-line distances. The main contribution is the trajectory design, optimized to avoid interdicted regions, to pass near predefined way-points, with guaranteed communication time, and to minimize total path length. Mixed-integer description is employed into the associated constrained optimization problem. The second novelty is the sensor localization and clustering method for optimal ground coverage taking into account the communication information between UAV and a subset of ground sensors (i.e., the cluster heads). Results show improvements in both network and data collection efficiency metrics by implementing the proposed algorithms. These are initially evaluated by means of simulation and then validated on a realistic WSN-UAV test-bed, thus bringing significant practical value.