bluuebunny/crossref_metadata_2025 huggingface dataset:
16S rRNA Gene Sequencing-Based Identification and Comparative Analysis of the Fecal Microbiota of Five Syntopic Lizard Species from a Low-Mountain Area in Western Bulgaria
Authors: Lazarkevich Irina , Engibarov Stephan , Mitova Simona , Vacheva Emiliya , Popova Steliyana , Stanchev Nikola , Eneva Rumyana , Gocheva Yana , Boyadzhieva Ivanka , Gerginova Maria
Published: January 2024

Studies on the gut microbiome of free-living reptiles in Europe are generally fragmentary and still missing in Bulgaria. We aimed to identify and compare the fecal microbiota profiles of five syntopic lizard species from three families: the European green lizard (Lacerta viridis), the common wall lizard (Podarcis muralis), the meadow lizard (Darevskia praticola) (Lacertidae), the European snake-eyed skink (Ablepharus kitaibelii) (Scincidae), and the European slow worm (Anguis fragilis) (Anguidae), which coinhabit a low mountainous area in the western part of the country. A high-throughput sequencing of the hypervariable V3-V4 region of the 16S rRNA gene, performed on the Illumina HiSeq2500 platform, was used. The core microbiota of lizard hosts seems to be species-specific. A dynamic phyla proportion between hosts was found. The richest alpha diversity was observed in D. praticola, and the lowest alpha diversity was observed in P. muralis and A. fragilis. Within the three lacertids, the microbiota of D. praticola and L. viridis were more closely related to each other than they were to those of P. muralis. Sharing a largely common trophic resource (all species except A. fragilis are mainly insectivorous) was not an indication of similarity in their gut microbial communities.
Toward Effects of Hydrophobicity on Biosurfactant Production by Bacillus subtilis Isolates from Crude-Oil-Exposed Environments
Authors: Hashemi Seyedeh Zahra , Fooladi Jamshid , Vahidinasab Maliheh , Hubel Philipp , Pfannstiel Jens , Pillai Evelina , Hrenn Holger , Hausmann Rudolf , Lilge Lars
Published: January 2024

Background: Due to their structural features, biosurfactants reveal promising physicochemical properties, making them interesting for various applications in different fields, such as the food, cosmetics, agriculture, and bioremediation sectors. In particular, the bioproduction of surfactin, one of the most potent microbially synthesized biosurfactant molecules, is of great interest. However, since the wild-type productivities are comparably low, stimulatory environmental conditions have to be identified for improved bioproduction This study aims to find a correlation between the hydrophobicity and production of the biosurfactant surfactin by B. subtilis isolates from crude-oil-contaminated soil and water. Methods: The surfactin production yield was characterized in adapted batch cultivations using high-performance thin-layer liquid chromatography (HPTLC). Defined hydrophobic environmental conditions were achieved by supplementation with hexadecane or polystyrene beads, and the effects on biosurfactant production were measured. Adaptations at the protein level were analyzed using mass spectrometry measurements. Results: The correlation between hydrophobicity and surfactin production was characterized using Bacillus subtilis strains ZH1 and P7 isolated from crude-oil-contaminated soil and water. Since these isolates show the biodegradation of crude oil and hexadecane as hydrophobic substrates, respectively, a first-time approach, using polystyrene beads, was applied to provide a hydrophobic environment. Interestingly, contrary to popular opinion, reduced biosurfactant production was determined. Using mass spectrometric approaches, the physiological effects of co-cultivation and the cellular response at the protein level were investigated, resulting in altered quantities of stress proteins and proteins involved in the carbon metabolism counter to polystyrene beads. Conclusions: Contrary to common opinion, increasing hydrophobicity does not have a stimulating effect, and even reduces the effect on the bioproduction of surfactin as the main biosurfactant using selected B. subtilis strains.
Constructing a Nomogram Model to Estimate the Risk of Ventilator-Associated Pneumonia for Elderly Patients in the Intensive Care Unit
Authors: Gan Wensi , Chen Zhihui , Tao Zhen , Li Wenyuan
Published: January 2024

Background: Ventilator-associated pneumonia (VAP) causes heavy losses in terms of finances, hospitalization, and death for elderly patients in the intensive care unit (ICU); however, the risk is difficult to evaluate due to a lack of reliable assessment tools. We aimed to create and validate a nomogram to estimate VAP risk to provide early intervention for high-risk patients. Methods: Between January 2016 and March 2021, 293 patients from a tertiary hospital in China were retrospectively reviewed as a training set. Another 84 patients were enrolled for model validation from April 2021 to February 2022. Least absolute shrinkage and selection operator (LASSO) regression and multivariable logistic regression analysis were employed to select predictors, and a nomogram model was constructed. The calibration, discrimination, and clinical utility of the nomogram were verified. Finally, a web-based online scoring system was created to make the model more practical. Results: The predictors were hypoproteinemia, long-term combined antibiotic use, intubation time, length of mechanical ventilation, and tracheotomy/intubation. The area under the curve (AUC) was 0.937 and 0.925 in the training and validation dataset, respectively, suggesting the model exhibited effective discrimination. The calibration curve demonstrated high consistency with the observed result and the estimated values. Decision curve analysis (DCA) demonstrated that the nomogram was clinically applicable. Conclusions: We have created a novel nomogram model that can be utilized to anticipate VAP risk in elderly ICU patients, which is helpful for healthcare professionals to detect patients at high risk early and adopt protective interventions.
Bismuth Nanoparticles Encapsulated in a Porous Carbon Skeleton as Stable Chloride-Storage Electrodes for Seawater Desalination
Authors: Dong Xiaoqing , Wang Ying , Zou Qian , Li Chaolin
Published: January 2024

Cost-effective bismuth (Bi) boasts a high theoretical capacity and exceptional selectivity towards Cl- ion storage, making it a promising material for desalination batteries (DBs). However, the substantial volume expansion and low conductivity severely hinder the cycling performance of Bi-based DBs. In this study, a carbon-layer-coated Bi nanocomposite (Bi@C) was synthesized by pyrolyzing a metal–organic framework (Bi-MOF) containing Bi using a straightforward method. The results show that the Bi@C synthesized under the condition of annealing at 700 °C for 2 h has the optimum properties. The Bi@C has good multiplication performance, and the desalination capacity is 106.1 mg/g at a high current density of 1000 mA/g. And the material exhibited a high desalination capacity of 141.9 mg/g at a current density of 500 mA/g and retained 66.9% of its capacity after 200 cycles. In addition, the Bi@C can operate at a wide range of NaCl concentrations from 0.05 to 2 mol/L. The desalination mechanism analysis of the Bi@C revealed that the carbon coating provides space for Bi particles to expand in volume, thereby mitigating the issues of electrode material powdering and shedding. Meanwhile, the porous carbon skeleton establishes electron and ion channels to enhance the electrode material’s conductivity. This research offers a promising strategy for the application of chloride-storage electrode materials in electrochemical desalination systems.
Resolving Genotype–Phenotype Discrepancies of the Kidd Blood Group System Using Long-Read Nanopore Sequencing
Authors: Gueuning Morgan , Thun Gian Andri , Trost Nadine , Schneider Linda , Sigurdardottir Sonja , Engström Charlotte , Larbes Naemi , Merki Yvonne , Frey Beat M. , Gassner Christoph , Meyer Stefan , Mattle-Greminger Maja P.
Published: January 2024

Due to substantial improvements in read accuracy, third-generation long-read sequencing holds great potential in blood group diagnostics, particularly in cases where traditional genotyping or sequencing techniques, primarily targeting exons, fail to explain serological phenotypes. In this study, we employed Oxford Nanopore sequencing to resolve all genotype–phenotype discrepancies in the Kidd blood group system (JK, encoded by SLC14A1) observed over seven years of routine high-throughput donor genotyping using a mass spectrometry-based platform at the Blood Transfusion Service, Zurich. Discrepant results from standard serological typing and donor genotyping were confirmed using commercial PCR-SSP kits. To resolve discrepancies, we amplified the entire coding region of SLC14A1 (~24 kb, exons 3 to 10) in two overlapping long-range PCRs in all samples. Amplicons were barcoded and sequenced on a MinION flow cell. Sanger sequencing and bridge-PCRs were used to confirm findings. Among 11,972 donors with both serological and genotype data available for the Kidd system, we identified 10 cases with unexplained conflicting results. Five were linked to known weak and null alleles caused by variants not included in the routine donor genotyping. In two cases, we identified novel null alleles on the JK*01 (Gly40Asp; c.119G>A) and JK*02 (Gly242Glu; c.725G>A) haplotypes, respectively. Remarkably, the remaining three cases were associated with a yet unknown deletion of ~5 kb spanning exons 9–10 of the JK*01 allele, which other molecular methods had failed to detect. Overall, nanopore sequencing demonstrated reliable and accurate performance for detecting both single-nucleotide and structural variants. It possesses the potential to become a robust tool in the molecular diagnostic portfolio, particularly for addressing challenging structural variants such as hybrid genes, deletions and duplications.
A Brain-Protective Sterol from Soft Coral Inhibits Lipopolysaccharide-Induced Matrix Metalloproteinase-9-Mediated Astrocytic Migration
Authors: Lee Tsong-Hai , Chen Jiun-Liang , Chang Chuan-Hsin , Tsai Ming-Ming , Tseng Hui-Ching , Chang Yu-Chia , Shanmugam Velayuthaprabhu , Hsieh Hsi-Lung
Published: January 2024

Matrix metalloproteinases (MMPs), which are proteolytic enzymes, promote blood–brain barrier (BBB) disruption, leading to neuronal damage and neuroinflammation. Among them, MMP-9 upregulation serves as an inflammatory biomarker in the central nervous system (CNS). Currently, the development of marine organism-derived bioactive compounds or metabolites as anti-inflammatory drugs has received considerable attention. The 9,11-secosteroid, 3β,11-dihydroxy-9,11-secogorgost-5-en-9-one (4p3f), is a novel sterol compound extracted from the soft coral Sinularia leptoclado with potential anti-inflammatory activity. However, the effect of and potential for brain protection of 4p3f on brain astrocytes remain unclear. Herein, we used rat brain astrocytes (RBAs) to investigate the effects and signaling mechanisms of 4p3f on lipopolysaccharide (LPS)-induced MMP-9 expression via zymographic, quantitative reverse transcription–polymerase chain reaction (qRT-PCR), Western blot, immunofluorescence staining, promoter–reporter, and cell migration analyses. We first found that 4p3f blocked LPS-induced MMP-9 expression in RBAs. Next, we demonstrated that LPS induced MMP-9 expression via the activation of ERK1/2, p38 MAPK, and JNK1/2, which is linked to the STAT3-mediated NF-κB signaling pathway. Finally, 4p3f effectively inhibited LPS-induced upregulation of MMP-9-triggered RBA cell migration. These data suggest that a novel sterol from soft coral, 4p3f, may have anti-inflammatory and brain-protective effects by attenuating these signaling pathways of MMP-9-mediated events in brain astrocytes. Accordingly, the soft coral-derived sterol 4p3f may emerge as a potential candidate for drug development or as a natural compound with neuroprotective properties.
A Bioactive Gelatin-Methacrylate Incorporating Magnesium Phosphate Cement for Bone Regeneration
Authors: Zhang Xiping , Gong Changtian , Wang Xingyu , Wei Zhun , Guo Weichun
Published: January 2024

Maintaining proper mechanical strength and tissue volume is important for bone growth at the site of a bone defect. In this study, potassium magnesium phosphate hexahydrate (KMgPO4·6H2O, MPC) was applied to gelma-methacrylate hydrogel (GelMA) to prepare GelMA/MPC composites (GMPCs). Among these, 5 GMPC showed the best performance in vivo and in vitro. These combinations significantly enhanced the mechanical strength of GelMA and regulated the degradation and absorption rate of MPC. Considerably better mechanical properties were noted in 5 GMPC compared with other concentrations. Better bioactivity and osteogenic ability were also found in 5 GMPC. Magnesium ions (Mg2+) are bioactive and proven to promote bone tissue regeneration, in which the enhancement efficiency is closely related to Mg2+ concentrations. These findings indicated that GMPCs that can release Mg2+ are effective in the treatment of bone defects and hold promise for future in vivo applications.
High-Grade Serous Ovarian Cancer—A Risk Factor Puzzle and Screening Fugitive
Authors: Wilczyński Jacek , Paradowska Edyta , Wilczyński Miłosz
Published: January 2024

High-grade serous ovarian cancer (HGSOC) is the most lethal tumor of the female genital tract. Despite extensive studies and the identification of some precursor lesions like serous tubal intraepithelial cancer (STIC) or the deviated mutational status of the patients (BRCA germinal mutation), the pathophysiology of HGSOC and the existence of particular risk factors is still a puzzle. Moreover, a lack of screening programs results in delayed diagnosis, which is accompanied by a secondary chemo-resistance of the tumor and usually results in a high recurrence rate after the primary therapy. Therefore, there is an urgent need to identify the substantial risk factors for both predisposed and low-risk populations of women, as well as to create an economically and clinically justified screening program. This paper reviews the classic and novel risk factors for HGSOC and methods of diagnosis and prediction, including serum biomarkers, the liquid biopsy of circulating tumor cells or circulating tumor DNA, epigenetic markers, exosomes, and genomic and proteomic biomarkers. The novel future complex approach to ovarian cancer diagnosis should be devised based on these findings, and the general outcome of such an approach is proposed and discussed in the paper.
Modulating TRPV4 Channel Activity in Pro-Inflammatory Macrophages within the 3D Tissue Analog
Authors: Babaniamansour Parto , Jacho Diego , Niedzielski Skyler , Rabino Agustin , Garcia-Mata Rafael , Yildirim-Ayan Eda
Published: January 2024

Investigating macrophage plasticity emerges as a promising strategy for promoting tissue regeneration and can be exploited by regulating the transient receptor potential vanilloid 4 (TRPV4) channel. The TRPV4 channel responds to various stimuli including mechanical, chemical, and selective pharmacological compounds. It is well documented that treating cells such as epithelial cells and fibroblasts with a TRPV4 agonist enhances the Ca2+ influx to the cells, which leads to secretion of pro-inflammatory cytokines, while a TRPV4 antagonist reduces both Ca2+ influx and pro-inflammatory cytokine secretion. In this work, we investigated the effect of selective TRPV4 modulator compounds on U937-differentiated macrophages encapsulated within three-dimensional (3D) matrices. Despite offering a more physiologically relevant model than 2D cultures, pharmacological treatment of macrophages within 3D collagen matrices is largely overlooked in the literature. In this study, pro-inflammatory macrophages were treated with an agonist, 500 nM of GSK1016790A (TRPV4(+)), and an antagonist, 10 mM of RN-1734 (TRPV4(−)), to elucidate the modulation of the TRPV4 channel at both cellular and extracellular levels. To evaluate macrophage phenotypic alterations within 3D collagen matrices following TRPV4 modulator treatment, we employed structural techniques (SEM, Masson’s trichrome, and collagen hybridizing peptide (CHP) staining), quantitative morphological measures for phenotypic assessment, and genotypic methods such as quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). Our data reveal that pharmacological modulation of the macrophage TRPV4 channel alters the cytoskeletal structure of macrophages and influences the 3D structure encapsulating them. Moreover, we proved that treating macrophages with a TRPV4 agonist and antagonist enhances the expression of pro- and anti-inflammatory genes, respectively, leading to the upregulation of surface markers CD80 and CD206. In the TRPV4(−) group, the CD206 gene and CD206 surface marker were significantly upregulated by 9- and 2.5-fold, respectively, compared to the control group. These findings demonstrate that TRPV4 modulation can be utilized to shift macrophage phenotype within the 3D matrix toward a desired state. This is an innovative approach to addressing inflammation in musculoskeletal tissues.
Cannabinoid and Serotonergic Systems: Unraveling the Pathogenetic Mechanisms of Stress-Induced Analgesia
Authors: Nocheva Hristina , Stoynev Nikolay , Vodenicharov Vlayko , Krastev Dimo , Krastev Nikolay , Mileva Milka
Published: January 2024

The perception of „stress” triggers many physiological and behavioral responses, collectively called the stress response. Such a complex process allows for coping with stress and also triggers severe pathology. Because of the multidirectional effect of stress on the body, multiple systems participate in its pathogenesis, with the endogenous cannabinoid and the serotoninergic ones among them. These two systems also take part in the pain perception decrease, known as stress-induced analgesia (SIA), which can then be taken as an indirect indicator of the stress response. The aim of our study was to study the changes in cold SIA (c-SIA) resulting from the exogenous activation of cannabinoid receptor type 1 (CB1) and 5-hydroxytryptamine (5-HT, serotonin) receptor type 1A (5-HT1A). Various combinations of agonists and/or antagonists of CB1 and 5-HT1A, before or after 1 h of cold exposure, were applied, since we presumed that the exogenous activation of the receptors before the cold exposure would influence the pathogenesis of the stress response, while their activation after the stressful trigger would influence the later development. Our results show that the serotonergic system “maintained” c-SIA in the pre-stress treatment, while the cannabinoids’ modulative effect was more prominent in the post-stress treatment. Here, we show the interactions of the two systems in the stress response. The interpretation and understanding of the mechanisms of interaction between CB1 and 5-HT1A may provide information for the prevention and control of adverse stress effects, as well as suggest interesting directions for the development of targeted interventions for the control of specific body responses.
In Vitro Studies on 3D-Printed PLA/HA/GNP Structures for Bone Tissue Regeneration
Authors: Negrescu Andreea-Mariana , Mocanu Aura-Cătălina , Miculescu Florin , Mitran Valentina , Constantinescu Andreea-Elena , Cimpean Anisoara
Published: January 2024

The successful regeneration of large-size bone defects remains one of the most critical challenges faced in orthopaedics. Recently, 3D printing technology has been widely used to fabricate reliable, reproducible and economically affordable scaffolds with specifically designed shapes and porosity, capable of providing sufficient biomimetic cues for a desired cellular behaviour. Natural or synthetic polymers reinforced with active bioceramics and/or graphene derivatives have demonstrated adequate mechanical properties and a proper cellular response, attracting the attention of researchers in the bone regeneration field. In the present work, 3D-printed graphene nanoplatelet (GNP)-reinforced polylactic acid (PLA)/hydroxyapatite (HA) composite scaffolds were fabricated using the fused deposition modelling (FDM) technique. The in vitro response of the MC3T3-E1 pre-osteoblasts and RAW 264.7 macrophages revealed that these newly designed scaffolds exhibited various survival rates and a sustained proliferation. Moreover, as expected, the addition of HA into the PLA matrix contributed to mimicking a bone extracellular matrix, leading to positive effects on the pre-osteoblast osteogenic differentiation. In addition, a limited inflammatory response was also observed. Overall, the results suggest the great potential of the newly developed 3D-printed composite materials as suitable candidates for bone tissue engineering applications.
Development of a Taxonomy for Causes of Changes in Construction Projects
Authors: Birgonul Zeynep , Budayan Cenk , Koc Kerim
Published: January 2024

Contracting parties in construction projects confront significant challenges due to changes. This is an inherent industry characteristic. Managing changes properly with the help of a taxonomy encompassing many of the causes of changes can have a longitudinal and positive effect on project performance, knowledge management, and stakeholder management. However, studies to date have failed to propose an in-depth taxonomy for change causes in construction projects. Therefore, a taxonomy for change causes that can be applied to different construction projects has been developed. First, a systematic literature review and desk study sessions were conducted to identify the initial list of the taxonomy components. Six case studies were then analyzed to reveal the change causes of these cases. Based on the extracted change causes from the literature review and case studies, a taxonomy was developed by conducting focus group discussions with six experts. In the next step, the applicability and validity of the refined taxonomy were evaluated through face-to-face interviews. As a result, a taxonomy with a three-level hierarchy was proposed. This taxonomy is divided into three levels with 13 main categories, 50 subcategories, and 52 change causes. The proposed taxonomy is expected to contribute to practice by reducing the frequency of changes through proactive management of potential changes and standardizing knowledge management practices for managing change.
Long-Term Impact of D2 Lymphadenectomy during Gastrectomy for Cancer: Individual Patient Data Meta-Analysis and Restricted Mean Survival Time Estimation
Authors: Aiolfi Alberto , Bona Davide , Bonitta Gianluca , Lombardo Francesca , Manara Michele , Sozzi Andrea , Schlanger Diana , Popa Calin , Cavalli Marta , Campanelli Giampiero , Biondi Antonio , Bonavina Luigi
Published: January 2024

Background: Debate exists concerning the impact of D2 vs. D1 lymphadenectomy on long-term oncological outcomes after gastrectomy for cancer. Methods: PubMed, MEDLINE, Scopus, and Web of Science were searched and randomized controlled trials (RCTs) analyzing the effect of D2 vs. D1 on survival were included. Overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS) were assessed. Restricted mean survival time difference (RMSTD) and 95% confidence intervals (CI) were used as effect size measures. Results: Five RCTs (1653 patients) were included. Overall, 805 (48.7%) underwent D2 lymphadenectomy. The RMSTD OS analysis shows that at 60-month follow-up, D2 patients lived 1.8 months (95% CI −4.2, 0.7; p = 0.14) longer on average compared to D1 patients. Similarly, 60-month CSS (1.2 months, 95% CI −3.9, 5.7; p = 0.72) and DFS (0.8 months, 95% CI −1.7, 3.4; p = 0.53) tended to be improved for D2 vs. D1 lymphadenectomy. Conclusions: Compared to D1, D2 lymphadenectomy is associated with a clinical trend toward improved OS, CSS, and DFS at 60-month follow-up.
Why Is Surgery Still Done after Concurrent Chemoradiotherapy in Locally Advanced Cervical Cancer in Romania?
Authors: Voinea Silviu Cristian , Bordea Cristian Ioan , Chitoran Elena , Rotaru Vlad , Andrei Razvan Ioan , Ionescu Sinziana-Octavia , Luca Dan , Savu Nicolae Mircea , Capsa Cristina Mirela , Alecu Mihnea , Simion Laurentiu
Published: January 2024

The incidence and mortality of cervical cancer are high in Romania compared to other European countries, particularly for locally advanced cervical cancer cases, which are predominant at the time of diagnosis. Widely accepted therapeutic guidelines indicate that the treatment for locally advanced cervical cancer consists of concurrent chemoradiotherapy (total dose 85–90 Gy at point A), with surgery not being necessary as it does not lead to improved survival and results in significant additional morbidity. In Romania, the treatment for locally advanced cervical cancer differs, involving lower-dose chemoradiotherapy (total dose 60–65 Gy at point A), followed by surgery, which, under these circumstances, ensures better local control. In this regard, we attempted to evaluate the role and necessity of surgery in Romania, considering that in our study, residual lesions were found in 55.84% of cases on resected specimens, especially in cases with unfavorable histology (adenocarcinoma and adenosquamous carcinoma). This type of surgery was associated with significant morbidity (28.22%) in our study. The recurrence rate was 24.21% for operated-on patients compared to 62% for non-operated-on patients receiving suboptimal concurrent chemotherapy alone. In conclusion, in Romania, surgery will continue to play a predominant role until radiotherapy achieves the desired effectiveness for local control.
The Role of SOX2 and SOX9 in Radioresistance and Tumor Recurrence
Authors: Barbosa Silvia , Laureano Natalia Koerich , Hadiwikarta Wahyu Wijaya , Visioli Fernanda , Bonrouhi Mahnaz , Pajdzik Kinga , Conde-Lopez Cristina , Herold-Mende Christel , Eidt Gustavo , Langie Renan , Lamers Marcelo Lazzaron , Stögbauer Fabian , Hess Jochen , Kurth Ina , Jou Adriana
Published: January 2024

Head and neck squamous cell carcinoma (HNSCC) exhibits considerable variability in patient outcome. It has been reported that SOX2 plays a role in proliferation, tumor growth, drug resistance, and metastasis in a variety of cancer types. Additionally, SOX9 has been implicated in immune tolerance and treatment failures. SOX2 and SOX9 induce treatment failure by a molecular mechanism that has not yet been elucidated. This study explores the inverse association of SOX2/SOX9 and their distinct expression in tumors, influencing the tumor microenvironment and radiotherapy responses. Through public RNA sequencing data, human biopsy samples, and knockdown cellular models, we explored the effects of inverted SOX2 and SOX9 expression. We found that patients expressing SOX2LowSOX9High showed decreased survival compared to SOX2HighSOX9Low. A survival analysis of patients stratified by radiotherapy and human papillomavirus brings additional clinical relevance. We identified a gene set signature comprising newly discovered candidate genes resulting from inverted SOX2/SOX9 expression. Moreover, the TGF-β pathway emerges as a significant predicted contributor to the overexpression of these candidate genes. In vitro findings reveal that silencing SOX2 enhances tumor radioresistance, while SOX9 silencing enhances radiosensitivity. These discoveries lay the groundwork for further studies on the therapeutic potential of transcription factors in optimizing HNSCC treatment.
Ceramics 3D Printing: A Comprehensive Overview and Applications, with Brief Insights into Industry and Market
Authors: Abdelkader Mohamed , Petrik Stanislav , Nestler Daisy , Fijalkowski Mateusz
Published: January 2024

3D printing enables the creation of complex and sophisticated designs, offering enhanced efficiency, customizability, and cost-effectiveness compared to traditional manufacturing methods. Ceramics, known for their heat resistance, hardness, wear resistance, and electrical insulation properties, are particularly suited for aerospace, automotive, electronics, healthcare, and energy applications. The rise of 3D printing in ceramics has opened new possibilities, allowing the fabrication of complex structures and the use of diverse raw materials, overcoming the limitations of conventional fabrication methods. This review explores the transformative impact of 3D printing, or additive manufacturing, across various sectors, explicitly focusing on ceramics and the different 3D ceramics printing technologies. Furthermore, it presents several active companies in ceramics 3D printing, proving the close relation between academic research and industrial innovation. Moreover, the 3D printed ceramics market forecast shows an annual growth rate (CAGR) of more than 4% in the ceramics 3D printing market, reaching USD 3.6 billion by 2030.
Ferrocene-Containing Gallic Acid-Derivative Modified Carbon-Nanotube Electrodes for the Fast Simultaneous and Selective Determination of Cytostatics from Aqueous Solutions
Authors: Motoc (m. Ilies) Sorina , Andelescu Adelina , Visan Alexandru , Baciu Anamaria , Szerb Elisabeta I. , Manea Florica
Published: January 2024

In this work, a ferrocene-containing gallic acid-derivative modified carbon-nanotube paste electrode (Gal-Fc-CNT), obtained through simple mechanical mixing, was studied for the fast simultaneous voltammetric determination of doxorubicin (DOX), capecitabine (CPB), and cyclophosphamide (CPP) as cytostatic indices based on their cumulative signals and the selective determination of DOX. The individual and simultaneous electrochemical behavior of DOX, CPB, and CPP, studied through cyclic voltammetry (CV) on the Gal-Fc-CNT paste electrode at various pHs and potential ranges, allowed for the development of a simple simultaneous determination method as a cytostatic index at a pH of 12 using square-wave voltammetry, which allowed for a better performance than reported electrodes for each individual cytostatic. A faster and selective detection of DOX, with a limit of detection of 75 ng·L−1, was achieved using square-wave voltammetry at a pH of 3. The good results obtained for the real tap water assessment indicated the applicability of the Gal-Fc-CNT paste electrode for practical applications (water samples).
Evolution of Microstructure and Mechanical Properties of Novel Al-Mg-Mn-Ag-Cr-Zr Alloy
Authors: Wang Huan , Liu Tao , Wu Yanli , Guo Cheng
Published: January 2024

In order to reinforce the mechanism of Ag in 5xxx aluminum alloys with low magnesium, research on the microstructure and mechanical properties of an Al-Mg-Mn-Ag-Cr-Zr alloy was conducted using optical microscopy (OM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), hardness measurement, and tensile testing. The as-cast microscopic structure of the alloy comprises the Al6(Mn, Fe) phase and the T-Mg32(Al, Ag)49 phase. Changes in the characteristics of the investigated alloy were clear during the aging process. Based on the findings obtained from TEM and SAED analysis, it was evident that the predominant strengthening phase during the peak-aged stage is the β″ phase, observed when the alloy is aged for 24 h at 160 °C. The β″ phase had a L12-type crystal lattice architecture and presented a completely coherent relevance with the Al-matrix. The lattice parameter, a, of the β″ phase was 0.408 nm. The mechanical properties of the peak-aged alloy increased greatly as compared to the as-quenched alloy. The tensile strength exhibited a rise from 410 MPa to 449 MPa, representing a 9.5% increase, while the yield strength demonstrated an increase from 185 MPa to 273 MPa, indicating a significant enhancement of 47.5%. The method used in the present study has solved the problem of 5xxx aluminum alloys not being heat treatable for strengthening to a significant degree, considerably improving the alloy strength. In addition, new methods and foundations for exploiting new-type Al-Mg based alloys and developing high-strength aluminum alloys are provided in this study.
X-ray Technologies for Astrophysics Missions Supported by the Italian Space Agency
Authors: Miliucci Marco , Volpe Angela , Fabiani Sergio , Feroci Marco , Latronico Luca , Macculi Claudio , Piro Luigi , D’Andrea Matteo , Gatti Flavio , Puccetti Simonetta , Soffitta Paolo , Cavazzuti Elisabetta
Published: January 2024

The Italian Space Agency plays a key role in the fulfillment of space missions, contributing to the scientific, technological and economic progress in Italy. The agency accomplishes space experiments by collaborating with scientific and industrial entities, supporting them in the realization of new projects able to achieve, over the last two decades, unprecedented results and obtention of fundamental information on the birth and evolution of the universe. The paper describes a selection of X-ray technologies developed by the synergy between the Italian Space Agency and its principal collaborators which contributed to the main scientific results achieved over the years, together with the latest advances addressed to the next astrophysics missions.
Artemis as Predictive Biomarker of Responsiveness to Preoperative Chemoradiotherapy in Patients with Locally Advanced Rectal Cancer
Authors: Liu Hai , Huang Runying , Shan Jingjing , Xie Xuyun , Wang Chongwei , Hu Peng , Sun Xiaonan
Published: January 2024

The aim of this study was to identify Artemis as a predictive biomarker for guiding preoperative chemoradiotherapy in locally advanced rectal cancer. The resection specimens were collected from 50 patients with rectal cancer who underwent preoperative chemoradiotherapy. Artemis expression in biopsy tissues was evaluated using immunohistochemical staining according to the percentage of positively stained cells combined with staining intensity. Among the 50 patients, 36 (72%) had a weakly positive Artemis protein expression, 10 (20%) had a moderately positive expression, and 4 (8%) showed a strongly positive expression. The criteria of magnetic resonance imaging tumor regression grade (mrTRG) and pathological rectal cancer regression grade (RCRG) were used to assess the tumor response to chemoradiotherapy. Correlation analysis shows that there is a significant negative correlation between high Artemis immunoscore and treatment response (r = −0.532, p < 0.001). The results imply that high Artemis expression was associated with poor treatment response. Our study suggested a potential role of Artemis as a predictive biomarker of the tumor response to preoperative chemoradiotherapy in patients with locally advanced rectal cancer.
Late Quaternary Paleoecology and Environmental History of the Hortobágy, an Alkaline Steppe in Central Europe
Authors: Szilágyi Gábor , Gulyás Sándor , Vári Tamás Zsolt , Sümegi Pál
Published: January 2024

Hungary’s first national park was created in 1973 in the Hortobágy area to protect Europe’s largest contiguous steppe area and its flora and fauna. The Hortobágy National Park—the Puszta was inscribed on the UNESCO World Heritage List as a cultural landscape in 1999. The park’s outstanding importance is due to the predominantly non-arboreal steppe vegetation, home to a unique bird fauna, and alkaline and chernozem soils with a complex, mosaic-like spatial structure. In addition, the landscape of Hortobágy has a pastoral history stretching back thousands of years. Several hypotheses have been put forward that suggest that the alkaline soils and the habitats that cover them were formed as a result of human activities related to river regulation that began in the second half of the 19th century. However, paleoecological and paleobiological studies over the last 30–40 years have pointed to the natural origin of the alkaline steppes, dating back to the end of the Ice Age. For thousands of years, human activities, in particular, grazing by domestic animals, hardly influenced the natural evolution of the area. The drainage of marshy and flooded areas began in the 19th century, as well as the introduction of more and more intensive agriculture, had a significant impact on the landscape. This paper aims to describe the past natural development of this special alkaline steppe ecosystem, with particular reference to the impacts of past and present human activities, including conservation measures.
Diagnosing Cutaneous Melanocytic Tumors in the Molecular Era: Updates and Review of Literature
Authors: Huang Chelsea , Lau Tiffany Wing-See , Smoller Bruce R.
Published: January 2024

Over the past decade, molecular and genomic discoveries have experienced unprecedented growth, fundamentally reshaping our comprehension of melanocytic tumors. This review comprises three main sections. The first part gives an overview of the current genomic landscape of cutaneous melanocytic tumors. The second part provides an update on the associated molecular tests and immunohistochemical stains that are helpful for diagnostic purposes. The third section briefly outlines the diverse molecular pathways now utilized for the classification of cutaneous melanomas. The primary goal of this review is to provide a succinct overview of the molecular pathways involved in melanocytic tumors and demonstrate their practical integration into the realm of diagnostic aids. As the molecular and genomic knowledge base continues to expand, this review hopes to serve as a valuable resource for healthcare professionals, offering insight into the evolving molecular landscape of cutaneous melanocytic tumors and its implications for patient care.
Late Complications in Long-Term Childhood Cancer Survivors: What the Oral Health Professional Needs to Know
Authors: Al-Ansari Sali , Stolze Juliette , Bresters Dorine , Brook Alan Henry , Laheij Alexa M. G. A. , Brand Henk S. , Dahllöf Göran , Rozema Frederik R. , Raber-Durlacher Judith E.
Published: January 2024

With diagnostic and therapeutic advances, over 80% of children diagnosed with cancer become long-term survivors. As the number of childhood cancer survivors (CCS) continues to increase, dental practitioners become more likely to have CCS among their patients. CCS may develop late complications from damage caused by their cancer treatment to endocrine, cardiovascular, musculoskeletal, and other organ systems. These complications may surface decades after the completion of treatment. Adverse outcomes of childhood cancer treatment frequently involve oral and craniofacial structures including the dentition. Tooth development, salivary gland function, craniofacial growth, and temporomandibular joint function may be disturbed, increasing oral health risks in these individuals. Moreover, CCS are at risk of developing subsequent malignancies, which may manifest in or near the oral cavity. It is important that dental practitioners are aware of the childhood cancer history of their patients and have knowledge of potential late complications. Therefore, this narrative review aims to inform dental practitioners of late oral complications of cancer treatment modalities commonly used in pediatric oncology. Furthermore, selected common non-oral late sequelae of cancer therapy that could have an impact on oral health and on delivering dental care will be discussed.
Identification of Critical Links Based on Electrical Betweenness and Neighborhood Similarity in Cyber-Physical Power Systems
Authors: Dong Jiuling , Song Zilong , Zheng Yuanshuo , Luo Jingtang , Zhang Min , Yang Xiaolong , Ma Hongbing
Published: January 2024

Identifying critical links is of great importance for ensuring the safety of the cyber-physical power system. Traditional electrical betweenness only considers power flow distribution on the link itself, while ignoring the local influence of neighborhood links and the coupled reaction of information flow on energy flow. An identification method based on electrical betweenness centrality and neighborhood similarity is proposed to consider the internal power flow dynamic influence existing in multi-neighborhood nodes and the topological structure interdependence between power nodes and communication nodes. Firstly, for the power network, the electrical topological overlap is proposed to quantify the vulnerability of the links. This approach comprehensively considers the local contribution of neighborhood nodes, power transmission characteristics, generator capacity, and load. Secondly, in communication networks, effective distance closeness centrality is defined to evaluate the importance of communication links, simultaneously taking into account factors such as the information equipment function and spatial relationships. Next, under the influence of coupled factors, a comprehensive model is constructed based on the dependency relationships between information flow and energy flow to more accurately assess the critical links in the power network. Finally, the simulation results show the effectiveness of the proposed method under dynamic and static attacks.
How Far Are NEET Youth Falling Behind in Their Non-Cognitive Skills? An Econometric Analysis of Disparities
Authors: Avanesian Garen , Borovskaya Marina , Masych Marina , Dikaya Ludmila , Ryzhova Victoria , Egorova Valeria
Published: January 2024

Youth not in education, employment, or training (NEET) refers to the most vulnerable group in the transition from school to work. While much research focuses on institutional factors behind the NEET incidence, the current study approaches the problem of the NEET youth from the perspective of non-cognitive skills. For measuring non-cognitive skills, the Big Five personality characteristics (openness, conscientiousness, extraversion, agreeableness, neuroticism) as well as grit were analyzed. The analysis was carried out using propensity score matching based on the data of the Russian Longitudinal Monitoring Survey (RLMS HSE) for 2016. This study shows that the majority of young people in the NEET group come from the poorest families. Nearly half of the NEET youth are not only not working, but they are also not looking for a job either. The analysis revealed that NEET youth fall behind in different non-cognitive abilities, with statistically significant differences in conscientiousness, extraversion, and grit, as well as a greater severity of neuroticism.
Effects of Physical Activity and COVID-19 on Healthy Student Strengths in the University System: Implications for Post-Pandemic Management
Authors: Gómez Chacón Ramón , Nuñez Sánchez Jose Manuel , Gálvez Ruiz Pablo
Published: January 2024

The COVID-19 pandemic has profoundly affected the physical, mental, and social well-being of millions worldwide. It has also brought about abrupt disruptions to the entire university system, whose students form a crucial segment of society. The pandemic’s effects on student education and well-being have been particularly significant. One of the primary consequences has been a drastic reduction in physical activity levels among students, leading to mental and physical health problems. Despite the rapid growth in the literature exploring student experiences during the pandemic, there is a paucity of research on how this decline in physical activity has affected the five strengths of the healthy student: optimism, self-efficacy, resilience, engagement, and hope. Therefore, the aim of this investigation is to examine the relationship between physical activity levels and the five strengths of the healthy student at two different time points (pre-COVID-19 and COVID-19) through the International Physical Activity Questionnaire (IPAQ) and the Healthy Student Questionnaire. The study involved 897 participants, with 290 participating in the pre-COVID-19 phase and 607 participating in the COVID-19 phase. The results revealed significant differences in the five strengths between the two periods. Students who engaged in physical activity exhibited significantly higher optimism scores in the pre-COVID-19 phase. During the COVID-19 phase, physically active students demonstrated significantly higher scores in optimism, resilience, and self-efficacy. These findings provide clear guidance for university administrators seeking to enhance student well-being in a post-pandemic world and in the face of future disruptions. Universities should consider implementing physical exercise programs for their students to promote psychosocial well-being and provide training and resources to equip faculty members with new skills to better understand and support students’ perceptions.
Towards Energy Efficient Wireless Sensing by Leveraging Ambient Wi-Fi Traffic
Authors: Sharma Aryan , Li Junye , Mishra Deepak , Jha Sanjay , Seneviratne Aruna
Published: January 2024

Wireless-based sensing of physical environments has garnered tremendous attention recently, and its applications range from intruder detection to environmental occupancy monitoring. Wi-Fi is positioned as a particularly advantageous sensing medium, due to the ubiquity of Wi-Fi-enabled devices in a more connected world. Although Wi-Fi-based sensing using Channel State Information (CSI) has shown promise, existing sensing systems commonly configure dedicated transmitters to generate packets for sensing. These dedicated transmitters substantially increase the energy requirements of Wi-Fi sensing systems, and hence there is a need for understanding how ambient transmissions from nearby Wi-Fi devices can be leveraged instead. This paper explores the potential of Wi-Fi-based sensing using CSI derived from ambient transmissions of Wi-Fi devices. We demonstrate that CSI sensing accuracy is dependent on the underlying traffic type and the Wi-Fi transceiver architecture, and that control packets yield more robust CSI than payload packets. We also show that traffic containing upload data is more suitable for human occupancy counting, using the Probability Mass Function (PMF) of CSI. We further demonstrate that multiple spatially diverse streams of Wi-Fi CSI can be combined for sensing to an accuracy of 99%. The experimental study highlights the importance of training Wi-Fi sensing systems for multiple transmission sources to improve accuracy. This research has significant implications for the development of energy-efficient Wi-Fi sensing solutions for a range of applications.
Progress and Challenges Connected with the Integration of Renewable Energy Sources with Railway Distribution Networks
Authors: Kuznetsov Valeriy , Hubskyi Petro , Rojek Artur , Udzik Magdalena , Lowczowski Krzysztof
Published: January 2024

Rail is the most efficient and low carbon means of transport, but efforts are still being made to improve the energy efficiency of this sector. In Poland, one of the elements of the implementation of the energy transformation in rail is the “Green Railway” program, which assumes an increase in the share of renewable energy sources (RES) in the power supply structure of the sector to 50% in 2025 and 85% in 2030, and ultimately, to 100%. An increase in energy efficiency leads to a reduction in financial costs, and also contributes to improving the environment and, consequently, to enhancing the economic and social benefit through a cost–benefit analysis. Energy consumption in railway operations is characterized (unlike in construction) by being repetitive. This energy consumption is produced in four areas: in the movement of trains; in auxiliary systems in the trains; in auxiliary systems in the infrastructure (lighting consumption of tunnels or sections of track, point heating systems, the signaling and communication systems, etc.); and in stations, workshops and by other consumers. The aim of this article was to review modern technologies using renewable energy sources in rail transport for traction and non-traction customers.