bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Distribution Characteristics and Influencing Factors of Traditional Villages in China
Authors: Bian Jiaojiao , Chen Wanxu , Zeng Jie
Published: April 2022

Traditional villages carry the essence of traditional culture, which is necessary for rural revitalisation. However, continuous urban expansion has resulted in the rapid decline and even disappearance of these villages in recent decades. It is necessary to analyse the spatial pattern and influencing factors for the protection and development of traditional villages. Previous studies focused on the value and theoretical protection mechanism of traditional villages in China, disregarding their spatial distribution characteristics and influencing factors. Thus, we employed a Geographic Information System and spatial analysis with mathematical statistics to analyse the characteristics of these villages. Moreover, we analysed the associated influencing factors both qualitatively and quantitatively. The results show that traditional villages were mainly distributed in the southeast of the Hu Line in China, with an unbalanced spatial distribution pattern and an agglomeration distribution tendency. In general, four major agglomeration areas of traditional villages formed at the junction of Hebei, Shandong, and Henan provinces; the border between the Guizhou, Guangxi, and Hunan provinces; the border between the Anhui, Zhejiang, and Jiangxi provinces; and northwestern and southeastern Yunnan provinces. Traditional villages also existed in areas with relief lower than 300 m, altitudes of less than 1000 m, and slopes of less than 10°. They were mostly distributed in subtropical and temperate zones. A positive correlation was found between traditional villages and the level of economic development, population, and human history; conversely, the transportation network was negatively correlated. This study reveals the complex and diverse characteristics of traditional villages and provides scientific suggestions for their future protection, development, and utilisation.
Seasonal Dynamics of Bacterial Community Structure in Diesel Oil-Contaminated Soil Cultivated with Tall Fescue (Festuca arundinacea)
Authors: Lee Yun-Yeong , Lee Soo , Lee Sang , Cho Kyung-Suk
Published: April 2022

The objective of this study was to explore the seasonal characteristics of rhizoremediation and the bacterial community structure over the course of a year in soil contaminated with diesel oil. The soil was contaminated with diesel oil at a total petroleum hydrocarbon (TPH) concentration of 30,000 mg-TPH·kg-soil−1. Tall fescue seedlings were planted in the contaminated soil and rhizoremediation performance was monitored for 317 days. The TPH concentration gradually declined, reaching 75.6% after day 61. However, the TPH removability decreased by up to 30% after re-contamination in the fall and winter. The bacterial community structure exhibited distinct seasonal dynamics. Genus Pseudomonas significantly increased up to 55.7% in the winter, while the genera Immundisolibacter and Lysobacter, well-known petroleum hydrocarbon (PH)-degrading bacteria, were found to be positively linked to the TPH removal rate. Consequently, knowledge of this seasonal variation in rhizoremediation performance and the bacterial community structure is useful for the improvement of rhizoremediation in PH-contaminated environments.
COVID-19 Phenotypes and Comorbidity: A Data-Driven, Pattern Recognition Approach Using National Representative Data from the United States
Authors: Vavougios George D. , Stavrou Vasileios T. , Konstantatos Christoforos , Sinigalias Pavlos-Christoforos , Zarogiannis Sotirios G. , Kolomvatsos Konstantinos , Stamoulis George , Gourgoulianis Konstantinos I.
Published: April 2022

The aim of our study was to determine COVID-19 syndromic phenotypes in a data-driven manner using the survey results based on survey results from Carnegie Mellon University’s Delphi Group. Monthly survey results (>1 million responders per month; 320,326 responders with a certain COVID-19 test status and disease duration <30 days were included in this study) were used sequentially in identifying and validating COVID-19 syndromic phenotypes. Logistic Regression-weighted multiple correspondence analysis (LRW-MCA) was used as a preprocessing procedure, in order to weigh and transform symptoms recorded by the survey to eigenspace coordinates, capturing a total variance of >75%. These scores, along with symptom duration, were subsequently used by the Two Step Clustering algorithm to produce symptom clusters. Post-hoc logistic regression models adjusting for age, gender, and comorbidities and confirmatory linear principal components analyses were used to further explore the data. Model creation, based on August’s 66,165 included responders, was subsequently validated in data from March–December 2020. Five validated COVID-19 syndromes were identified in August: 1. Afebrile (0%), Non-Coughing (0%), Oligosymptomatic (ANCOS); 2. Febrile (100%) Multisymptomatic (FMS); 3. Afebrile (0%) Coughing (100%) Oligosymptomatic (ACOS); 4. Oligosymptomatic with additional self-described symptoms (100%; OSDS); 5. Olfaction/Gustatory Impairment Predominant (100%; OGIP). Our findings indicate that the COVID-19 spectrum may be undetectable when applying current disease definitions focusing on respiratory symptoms alone.
Incidence and Predictors of Loss to Follow Up among Patients Living with HIV under Dolutegravir in Bunia, Democratic Republic of Congo: A Prospective Cohort Study
Authors: Buju Roger T. , Akilimali Pierre Z. , Kamangu Erick N. , Mesia Gauthier K. , Kayembe Jean Marie N. , Situakibanza Hippolyte N.
Published: April 2022

This study aimed to examine the incidence and predictors of loss to follow up (LTFU) in the context of ongoing atrocities caused by armed conflict, where HIV treatment programs and HIV-infected patients may face unique challenges in terms of ART adherence and retention in care. We conducted an observational prospective cohort study of 468 patients living with HIV (PLWHIV) under dolutegravir (DTG) in all health facilities in Bunia between July 2019 and July 2021. Kaplan–Meier plots were used to determine the probability of LTFU as a function of time as inclusive of the cohort. The main outcome variable was LTFU, defined as not taking an ART refill for a period of 3 months or longer from the last attendance for refill, and not yet classified as ‘dead’ or ‘transferred-out.’ The log-rank test was used to compare survival curves based on predictors. Cox proportional hazard modeling was used to measure predictors of LTFU from the baseline until 31 July 2021 (the endpoint). A total of 3435.22 person-months (p-m) were involved in follow up, with an overall incidence rate of 33.48 LTFU per 1000 p-m. Patients who had less experience with ART at enrolment and the ethnically Sudanese, had a higher hazard of being LTFU compared to their reference groups. This study reports a high LTFU rate in this conflict setting. An ART program in such a setting should pay more attention to naive patients and other particularly vulnerable patients such as Sudanese during the pre-ART phase. The study implies the implementation of innovative strategies to address this high risk of being LTFU, reducing either the cost or the distance to the health facility.
Communicating about Energy Policy in a Resource-Rich Jurisdiction during the Climate Crisis: Lessons from the People of Brisbane, Queensland, Australia
Authors: Selvey Linda A. , Carpenter Morris , Lazarou Mattea , Cullerton Katherine
Published: April 2022

There is a high degree of expert consensus that anthropogenic climate change will be catastrophic if urgent and significant measures to reduce carbon emissions are not undertaken worldwide. Australia is a world-leading exporter of coal and gas, and does not have an effective emissions reduction strategy. Though many Australians support action on climate change, this has not affected voting patterns. In this qualitative study, we aimed to explore the attitudes of Australian voters in Brisbane, Queensland, Australia towards potential environmental policies. We approached people in public spaces, and invited them to participate in interviews. Six of the thirty-five interview participants who voted for the two main political parties or were undecided voters agreed with the transition to 100% renewables and/or no new coal mines in Australia. Many thought that renewables were not reliable enough and/or the economy was too dependent on coal to make the transition. There was strong support for political leadership in order to regulate mining and pollution, and for a transition plan for fossil-fuel-dependent communities. Participants were most concerned about tangible environmental issues, such as waste and pollution, and also described needing clear solutions in order to engage with the issues. Some described feeling ‘shouted at’ by protests and messaging about climate change and environmental issues. Our findings suggest that solution-based messaging may increase levels of engagement about climate change, and that waste and pollution can be entry-points for discussions about climate change. It is important to have conversations with people about this important issue.
Effect of High-Intensity Interval Training Combined with Fasting in the Treatment of Overweight and Obese Adults: A Systematic Review and Meta-Analysis
Authors: Guo Zhicheng , Cai Jianguang , Wu Ziqiang , Gong Weiqi
Published: April 2022

Objectives: A systematic review and meta-analysis is conducted to compare the effects of high-intensity interval training (HIIT) combined with fasting (HIIT + fasting) and other interventions (HIIT alone, fasting alone, or normal intervention) in adults with overweight and obesity on body composition (body mass, body mass index (BMI), waist circumference (WC), percent fat mass (PFM), fat mass (FM), fat-free mass (FFM)), maximal oxygen uptake (VO2peak), and glucose metabolism (fasting plasma glucose (FPG)), fasting plasma insulin (FPI)). Methods: The databases of PubMed, the Cochrane Library, Embace, Web of Science, CNKI, Wangfang Data, and CBM were searched from their inception to February 2022. Randomized controlled trials comparing the effects of HIIT + fasting and other interventions on adults with overweight and obesity were included in this meta-analysis. The risk of bias was assessed by the Cochrane risk of bias tool. The effect size was completed by using mean difference (MD) and standard deviation. If there were varying units or large differences among the included studies, the standardized mean difference (SMD) would be used. The certainty of evidence was evaluated using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Results: Nine randomized controlled trials with 230 overweight and obese adults were conducted in accordance with our inclusion criteria. The results of the meta-analysis revealed that compared to the control group HIIT + fasting had better effects on the body mass, WC, FM, and VO2peak, while there were no significant differences in PFM, FFM, FPG, and FPI. Conclusions: Despite the number of included trials being small and the GRADE of all outcomes being very low, HIIT + fasting has a positive effect on the body composition of overweight and obese adults, and significantly improves VO2peak. For adults with overweight and obesity who have long-term comorbidity, HIIT + fasting was a better way to improve FPG than HIIT alone or fasting alone. More studies are required to investigate different combinations of HIIT + fasting; and the safety of HIIT + fasting intervention on overweight and obese adults.
Psychometrics of the Pearlin Mastery Scale among Family Caregivers of Older Adults Who Require Assistance in Activities of Daily Living
Authors: Lim Zhi Xiang , Chua Wei Ling , Lim Wee Shiong , Lim An Qi , Chua Kia Chong , Chan Ee-Yuee
Published: April 2022

This study examined the psychometric properties of the seven-item mastery scale among 392 family caregivers of care dependent older adults in a tertiary hospital in Singapore. Item response theory (IRT) analysis and confirmatory factor analysis (CFA) were used to assess the scale’s psychometric properties. Construct validity was assessed based on correlations between mastery and caregiver burden, depression, and quality of life. Data from the seven-item mastery scale showed acceptable reliability and model fit while IRT analysis showed that response categories were ordered but reflected poor fit for the two positively worded items. Without these two items, responses on the five-item version showed acceptable model fit and had acceptable reliability and high correlation with those on the seven-item version. Item responses on both the seven- and five-item versions show logical correlations with carer self-report on burden, depression, and quality of life. Further psychometric studies of the seven-item mastery scale are warranted. For practical applications such as caregiver screening during hospital admissions, the five-item mastery scale is fit for purpose.
The Temporal and Spatial Distributions and Influencing Factors of Transboundary Pollution in China
Authors: Zhang Zhonghao , Nie Tiantian , Wu Yingtao , Ling Jiahui , Huang Danhuang
Published: April 2022

Transboundary pollution between neighboring regions seriously affects the efficiency of overall environmental governance; however, there are few studies focused on how to estimate the degree of transboundary pollution between different regions. With China as a case study, this article developed a new measurement to estimate the degree of transboundary pollution among regions, and comprehensively investigated the influencing factors of transboundary pollution in China between 2000 and 2013. The results indicate that transboundary pollution effects exist in China. In ascending order, the regions most affected overall by transboundary pollution from polluting enterprises were as follows: eastern region < central region < western region. The reduction in transboundary pollution effects was most prominent for severely polluting enterprises in the eastern and western regions and lightly polluting enterprises in the central region. An analysis of the influencing factors reveals that the regional environmental regulation intensity has a negative feedback effect on the transboundary pollution effects. These findings indicate that polluting enterprises in regions with a low environmental regulation intensity are more inclined to operate in border areas to obtain both the environmental benefits associated with the low local environmental regulation intensity and the market economy benefits associated with neighboring urban regions, thereby aggravating environmental pollution in border areas.
Prevention of Non-Contact Anterior Cruciate Ligament Injuries among Youth Female Athletes: An Umbrella Review
Authors: Mattu Anmol T. , Ghali Brianna , Linton Vanessa , Zheng Alex , Pike Ian
Published: April 2022

Anterior cruciate ligament (ACL) injuries account for a large percentage of knee injuries, disproportionately affecting female athletes. To help health professionals stay current, we performed an umbrella review to evaluate the effectiveness of ACL injury prevention programs in reducing non-contact ACL injury rates, determine the effective components within interventions, and provide clinical recommendations. Twelve databases (Medline, Embase, Cochrane Database of Systematic Reviews, SPORTDiscus, Cumulative Index to Nursing and Allied Health Literature, PEDro, Web of Science Core Collection, Epistemonikos, TRIP, BC Guidelines and Protocols, CPG Infobase, ProQuest Dissertations and Theses Global) were searched in May 2021 to identify relevant systematic reviews and meta-analyses. Four databases were searched again in September 2021 to identify recent primary literature. Non-contact ACL injury data were extracted to calculate incidence rate ratios (IRRs) and these were combined using an inverse variance random-effects model. A qualitative assessment of included reviews was performed. The methodological quality of the studies was assessed using a Measurement Tool to Assess Systematic Reviews 2 (AMSTAR 2) or Cochrane Risk-of-Bias Tool for Randomized Trials (RoB 2). Sixteen reviews and two primary studies met the inclusion criteria. Across 11 primary studies, prevention programs were effective in reducing non-contact ACL injuries by 64% (IRR = 0.36 (95% CI: 0.18–0.70)). A multi-faceted exercise program, beginning in the pre-season and containing at least three exercise types, may be beneficial in reducing ACL injury risk.
Risk Perception towards COVID-19: A Systematic Review and Qualitative Synthesis
Authors: Cipolletta Sabrina , Andreghetti Gabriela , Mioni Giovanna
Published: April 2022

Several studies have attempted to identify how people’s risk perceptions differ in regard to containing COVID-19 infections. The aim of the present review was to illustrate how risk awareness towards COVID-19 predicts people’s preventive behaviors and to understand which features are associated with it. For the review, 77 articles found in six different databases (ProQuest, PsycInfo, PubMed, Science Direct, SCOPUS, and Web of Science) were considered, the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was followed, and data synthesis was conducted using a mixed-methods approach. The results indicate that a high-risk perception towards COVID-19 predicts, in general, compliance with preventive behaviors and social distancing measures. Additionally, risk awareness was found to be associated with four other key themes: demographic factors, individual factors, geographical factors, and timing. Therefore, gaining a greater understanding of individual and cultural differences as well as how people behave could be the basis of an effective strategy for raising public risk awareness and for countering COVID-19.
Living with Rheumatic Heart Disease at the Intersection of Biomedical and Aboriginal Worldviews
Authors: Haynes Emma , Marawili Minitja , Marika Makungun B. , Mitchell Alice , Walker Roz , Katzenellenbogen Judith M. , Bessarab Dawn
Published: April 2022

Rheumatic heart disease (RHD) significantly impacts the lives of First Nations Australians. Failure to eliminate RHD is in part attributed to healthcare strategies that fail to understand the lived experience of RHD. To rectify this, a PhD study was undertaken in the Northern Territory (NT) of Australia, combining Aboriginal ways of knowing, being and doing with interviews (24 participants from clinical and community settings) and participant observation to privilege Aboriginal voices, including the interpretations and experiences of Aboriginal co-researchers (described in the adjunct article). During analysis, Aboriginal co-researchers identified three interwoven themes: maintaining good feelings; creating clear understanding (from good information); and choosing a good djalkiri (path). These affirm a worldview that prioritises relationships, positive emotions and the wellbeing of family/community. The findings demonstrate the inter-connectedness of knowledge, choice and behaviour that become increasingly complex in stressful and traumatic health, socioeconomic, political, historical and cultural contexts. Not previously heard in the RHD domain, the findings reveal fundamental differences between Aboriginal and biomedical worldviews contributing to the failure of current approaches to communicating health messages. Mitigating this, Aboriginal co-researchers provided targeted recommendations for culturally responsive health encounters, including: communicating to create positive emotions; building trust; and providing family and community data and health messages (rather than individualistic).
The Effect of an Educational Intervention on Self-Care in Patients with Venous Leg Ulcers—A Randomized Controlled Trial
Authors: Žulec Mirna , Rotar Pavlič Danica , Žulec Ana
Published: April 2022

Background: Although patients with venous leg ulcers are involved in ulcer management, little is known about why and how these patients self-treat their ulcers without direct supervision by health professionals. Yet patients’ knowledge of ulcer management can be important for achieving ulcer closure and/or preventing recurrence. This study thus investigates the effects of an educational intervention on knowledge of self-care among patients with venous leg ulcers, mainly on wound dressing practice, compression therapy, physical activity and nutrition. Methods and participants: This research was conducted in three outpatient hospitals in central Croatia. An educational brochure was made and distributed to patients; patients were surveyed about caring for venous leg ulcers before the brochure was distributed and after 3 months. Results: In total, 208 patients were involved in the study: 112 in the experimental group and 96 in the control group. The educational intervention increased awareness of compression therapy, knowledge of recurrence prevention, appropriate lifestyle habits, and warning signs related to venous leg ulcers. Conclusions: Patient education on illness and self-care is necessary to achieve positive effects in self-care knowledge. In this study, patients learned how to change dressings, learned how to improve their lifestyle, and were empowered to deal with their illness.
Children’s Filial Piety Changes Life Satisfaction of the Left-Behind Elderly in Rural Areas in China?
Authors: Luo Yaling , Wu Xiling , Liao Liao , Zou Hongmei , Zhang Lulu
Published: April 2022

Along with the aging of the population and miniaturization of family structure, the problem of the left-behind elderly has become more and more prominent in China. According to the Report on the family development in China (2015) released by the National Health Commission of the People’s Republic of China, left-behind elderly people account for half of the total number of the elderly, of whom 10% live alone. The left-behind elderly not only suffer physiological obstacles such as body function decline, but also face a lack of support at the material level and loneliness at the spiritual level, which greatly affects their quality of life, accounting for their lower life satisfaction than that of the ordinary elderly. The rural areas of Sichuan Province are relatively backwards in terms of economic level and have limited pension security. Therefore, the left-behind elderly in rural areas are confronted with more complicated and severe pension problems compared with those in urban areas. Meanwhile, limited by economic and regional factors, a large number of rural labor forces in Sichuan Province have transferred to cities. These long-time migrant workers cannot provide material, spiritual and life care support for their left-behind parents in rural areas in a timely fashion, which changes their filial piety behaviors, and this affects the life satisfaction of the rural left-behind elderly. Therefore, it is necessary to understand the living conditions of empty-nest elderly and their children’s filial piety in rural areas of Sichuan province in order to verify the influence mechanism of filial piety on the life satisfaction of the elderly, and to explore how to improve the rural empty-nest elderly’s life satisfaction, enabling the elderly to live a healthy and happy life.
Rotaviruses and Noroviruses as Etiological Agents of Acute Intestinal Diseases of Ukrainian Children
Authors: Soloviov Serhii O. , Todosiichuk Tetiana S. , Kovaliuk Olena V. , Filippelli Gabriel M. , Trokhymenko Olena P. , Dziublyk Iryna V. , Rodd Zachary A.
Published: April 2022

(1) Background: Rotavirus and norovirus infections are the primary viral causes of childhood diarrhea. In Ukraine, the diarrhea-linked infant mortality rate is low, but the number of children infected is quite high. This study examined the rates of rotavirus and norovirus infections throughout Ukraine. (2) Methods: Fecal samples for children admitted to hospitals in six Ukrainian cities (Kyiv, Lviv, Sumy, Odesa, Kharkiv, and Uman) were tested for the presence of rotavirus and norovirus. (3) Results: The overall rate of hospitalized children suffering from diarrhea with confirmed presence of rotavirus or norovirus in fecal samples was significant (20.67% and 27.94%, respectively). Samples obtained from children from Lviv had significantly higher rates of the viruses, and Kyiv and Uman had significantly lower rotavirus or norovirus detection levels than expected. (4) Conclusion: Childhood diarrhea impacts Ukraine significantly. The economic and societal effects of the failure to address this public health issue are indicated by the hospitalization rate of children with preventable illnesses. The geographical disparities in Ukraine for child hospitalizations caused by rotavirus and norovirus infections could result from environmental (sanitary factors or water purity issues) or social factors. Further research is needed to completely characterize infant viral infections in Ukraine.
A Pilot Dance Intervention to Encourage Physical Activity Engagement for Adolescent Girls with Intellectual Disabilities
Authors: Must Aviva , Bandini Linda G. , Curtin Carol , Rancaño Katherine M. , Eliasziw Misha , Tybor DJ , Stanish Heidi
Published: April 2022

Adolescent girls with intellectual disabilities (ID) are at risk for low physical activity (PA) participation due to their limited opportunities. Purpose: To evaluate the feasibility and preliminary efficacy of a 12-week dance intervention to promote engagement in moderate-to-vigorous PA (MVPA) and increase cardiorespiratory fitness. Methods: The 12-week intervention included two 75-min weekly dance sessions. Continuous heart-rate (HR) monitoring assessed time spent below/at/above each girl’s target HR zone. Cardiorespiratory fitness was measured by the 6-min walk test (6MWT). Survey items assessed participant enjoyment and participant and parent satisfaction. Results: The mean (SD) age of 18 adolescent girls was 17.3 (2.7) years. Overall, girls attended 88% of sessions and spent 52.3% of each session in MVPA. Mean MVPA was unchanged across the 12 weeks, but the pattern differed across the three sites. We observed a non-significant pre-post increase of 74.6 feet on the 6MWT. Post-intervention surveys indicated that most girls liked the program, perceived improved fitness, and wished to continue dancing. The majority also reported a preference for a girls-only dance program exclusively for those with ID. Conclusion: Our findings suggest that dance is viable for promoting PA for girls with ID. More frequent exercise training is likely needed to improve cardiorespiratory fitness.
Assessing Nursing Students’ Self-Perceptions about Safe Medication Management: Design and Validation of a Tool, the NURSPeM
Authors: Fuster-Linares Pilar , Alfonso-Arias Cristina , Gallart Fernández-Puebla Alberto , Rodríguez-Higueras Encarna , García-Mayor Silvia , Font-Jimenez Isabel , Llaurado-Serra Mireia
Published: April 2022

Objective: The objective was to design and validate a tool for assessing nursing students’ self-perceptions about safe medication management. Methods: A descriptive instrumental study was conducted involving construct definition, development of the tool, analysis of the content validity, and psychometric evaluation. Consensus regarding the content was obtained through a two-round Delphi process, and the resulting tool (the NURSPeM) was administered to nursing students to examine its internal consistency, temporal stability, and construct validity, the latter through exploratory factor analysis. Results: Thirteen experts participated in the Delphi process, which yielded a tool comprising two questionnaires: (1) Self-perceptions about safe medication management (27 items) and (2) the frequency and learning of drug-dose calculation (13 items). The tool’s psychometric properties were then examined based on responses from 559 nursing students. This analysis led to the elimination of three items from questionnaire 1, leaving a total of 24 items distributed across seven dimensions. All 13 items in questionnaire 2 were retained. Both questionnaires showed good internal consistency (Cronbach’s alpha = 0.894 and 0.893, respectively) and temporal stability (ICC = 0.894 and 0.846, respectively). Conclusions: The NURSPeM is a valid and reliable tool for assessing nursing students’ self-perceptions about safe medication management. It may be used to identify areas in which their training needs to be enhanced, and to evaluate the subsequent impact of new teaching initiatives.
Physical Activity, Climate Change and Health—A Conceptual Model for Planning Public Health Action at the Organizational Level
Authors: Schneider Sven , von Winning Alexandra , Grüger Fiona , Anderer Stefan , Hoffner Robert , Anderson Lilian
Published: April 2022

Climate change is linked to health risks for both professional and amateur athletes. Sports organisations will need to react to these developments. The starting point for this concept paper is a summary of the sport-specific health risks currently under discussion: increasing heatwaves, growing numbers of extreme weather events, rising UV, ozone and allergen levels and the spread of infectious diseases. Based on the current state of research, a conceptual model is developed to reduce these climate-related health risks in sports at organisational level. Given the wide variety of predicted direct and indirect health risks linked to climate change, the “sports, clubs and climate change model” (SC3 model) presented here follows a stepwise risk-specific approach using technical, organisational and person-related measures. The SC3 model also includes cross-cutting measures that have an overarching effect comprising training, warning systems, coordination and evaluation measures. The SC3 model makes it possible to develop prevention plans, both at national level for central associations and at the regional level of local organisations and clubs. It can be applied to typical settings (e.g., training or competition at elite or amateur levels) and target groups (e.g., athletes, spectators, referees and club officials).
An Overview of Antibiotic Resistance and Abiotic Stresses Affecting Antimicrobial Resistance in Agricultural Soils
Authors: Kaviani Rad Abdullah , Astaykina Angelika , Streletskii Rostislav , Afsharyzad Yeganeh , Etesami Hassan , Zarei Mehdi , Balasundram Siva K.
Published: April 2022

Excessive use of antibiotics in the healthcare sector and livestock farming has amplified antimicrobial resistance (AMR) as a major environmental threat in recent years. Abiotic stresses, including soil salinity and water pollutants, can affect AMR in soils, which in turn reduces the yield and quality of agricultural products. The objective of this study was to investigate the effects of antibiotic resistance and abiotic stresses on antimicrobial resistance in agricultural soils. A systematic review of the peer-reviewed published literature showed that soil contaminants derived from organic and chemical fertilizers, heavy metals, hydrocarbons, and untreated sewage sludge can significantly develop AMR through increasing the abundance of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARBs) in agricultural soils. Among effective technologies developed to minimize AMR’s negative effects, salinity and heat were found to be more influential in lowering ARGs and subsequently AMR. Several strategies to mitigate AMR in agricultural soils and future directions for research on AMR have been discussed, including integrated control of antibiotic usage and primary sources of ARGs. Knowledge of the factors affecting AMR has the potential to develop effective policies and technologies to minimize its adverse impacts.
A Pre–Post Study on the Cardiorespiratory Response to Different Protocols of Exposure on a Vibratory Platform in Young Healthy Individuals
Authors: Iconaru Elena Ioana , Ciucurel Manuela Mihaela , Georgescu Luminita , Tudor Mariana , Tantu Monica Marilena , Ciucurel Constantin
Published: April 2022

This study aimed to investigate the acute specific physiological effects of 15 min of whole-body vibration (WBV) exposure at six different types of vibrations on cardiorespiratory function in 26 healthy young subjects (sex ratio, 1:1; mean age, 20.73 years). The protocols included six variants of a combination of mechanical stimuli with different frequencies (15, 25, and 35 Hz) and direction of stimuli (vertical or diagonal). The investigated cardiorespiratory parameters were heart rate (HR), arterial oxygen saturation (SaO2), respiratory rate (RR), and spirometric indicators: tidal volume (TV), vital capacity (VC), forced vital capacity (FVC), forced expiratory volume at 1 s (FEV1), and maximum voluntary ventilation for 12 s (MVV). The data series were statistically processed by using descriptive and inferential statistical methods: the Shapiro–Wilk test, the two-way ANOVA with repeated measures, and post hoc analysis. We obtained significantly higher values for HR, TV, VC, FVC, FEV1, and MVV after the WBV exposure. These parameters are significantly influenced by both the frequency and direction of stimuli, and certain protocols of WBV are noticeable for their distinct effects. Our results offer a new perspective on the possibility of using preferential variants of vibratory stimulation to obtain maximum cardiorespiratory physiological effects.
Identification of Small Regions of Overlap from Copy Number Variable Regions in Patients with Hypospadias
Authors: Scott Carter H. , Amarillo Ina E.
Published: April 2022

Hypospadias is a common form of congenital atypical sex development that is often associated with other congenital comorbidities. Many genes have been associated with the condition, most commonly single sequence variations. Further investigations of recurrent and overlapping copy number variations (CNVs) have resulted in the identification of genes and chromosome regions associated with various conditions, including differences of sex development (DSD). In this retrospective study, we investigated the DECIPHER database, as well as an internal institutional database, to identify small recurrent CNVs among individuals with isolated and syndromic hypospadias. We further investigated these overlapping recurrent CNVs to identify 75 smallest regions of overlap (SROs) on 18 chromosomes. Some of the genes within these SROs may be considered potential candidate genes for the etiology of hypospadias and, occasionally, additional comorbid phenotypes. This study also investigates for the first time additional common phenotypes among individuals with hypospadias and overlapping CNVs. This study provides data that may aid genetic counseling and management of individuals with hypospadias, as well as improve understanding of its underlying genetic etiology and human genital development overall.
Tracing the Primordial Chemical Life of Glycine: A Review from Quantum Chemical Simulations
Authors: Rimola Albert , Balucani Nadia , Ceccarelli Cecilia , Ugliengo Piero
Published: April 2022

Glycine (Gly), NH2CH2COOH, is the simplest amino acid. Although it has not been directly detected in the interstellar gas-phase medium, it has been identified in comets and meteorites, and its synthesis in these environments has been simulated in terrestrial laboratory experiments. Likewise, condensation of Gly to form peptides in scenarios resembling those present in a primordial Earth has been demonstrated experimentally. Thus, Gly is a paradigmatic system for biomolecular building blocks to investigate how they can be synthesized in astrophysical environments, transported and delivered by fragments of asteroids (meteorites, once they land on Earth) and comets (interplanetary dust particles that land on Earth) to the primitive Earth, and there react to form biopolymers as a step towards the emergence of life. Quantum chemical investigations addressing these Gly-related events have been performed, providing fundamental atomic-scale information and quantitative energetic data. However, they are spread in the literature and difficult to harmonize in a consistent way due to different computational chemistry methodologies and model systems. This review aims to collect the work done so far to characterize, at a quantum mechanical level, the chemical life of Gly, i.e., from its synthesis in the interstellar medium up to its polymerization on Earth.
Early Life Events and Maturation of the Dentate Gyrus: Implications for Neurons and Glial Cells
Authors: Aniol Viktor , Manolova Anna , Gulyaeva Natalia
Published: April 2022

The dentate gyrus (DG), an important part of the hippocampus, plays a significant role in learning, memory, and emotional behavior. Factors potentially influencing normal development of neurons and glial cells in the DG during its maturation can exert long-lasting effects on brain functions. Early life stress may modify maturation of the DG and induce lifelong alterations in its structure and functioning, underlying brain pathologies in adults. In this paper, maturation of neurons and glial cells (microglia and astrocytes) and the effects of early life events on maturation processes in the DG have been comprehensively reviewed. Early postnatal interventions affecting the DG eventually result in an altered number of granule neurons in the DG, ectopic location of neurons and changes in adult neurogenesis. Adverse events in early life provoke proinflammatory changes in hippocampal glia at cellular and molecular levels immediately after stress exposure. Later, the cellular changes may disappear, though alterations in gene expression pattern persist. Additional stressful events later in life contribute to manifestation of glial changes and behavioral deficits. Alterations in the maturation of neuronal and glial cells induced by early life stress are interdependent and influence the development of neural nets, thus predisposing the brain to the development of cognitive and psychiatric disorders.
A Novel NOX Inhibitor Treatment Attenuates Parkinson’s Disease-Related Pathology in Mouse Models
Authors: Ghosh Anurupa A. , Verma Dinesh Kumar , Cabrera Gabriela , Ofori Kwadwo , Hernandez-Quijada Karina , Kim Jae-Kwan , Chung Joo Hee , Moore Michael , Moon Sung Hwan , Seo Jong Bok , Kim Yong-Hwan
Published: April 2022

Parkinson’s disease (PD) is a progressive neurodegenerative motor disorder without an available therapeutic to halt the formation of Lewy bodies for preventing dopaminergic neuronal loss in the nigrostriatal pathway. Since oxidative-stress-mediated damage has been commonly reported as one of the main pathological mechanisms in PD, we assessed the efficacy of a novel NOX inhibitor from AptaBio Therapeutics (C-6) in dopaminergic cells and PD mouse models. The compound reduced the cytotoxicity and enhanced the cell viability at various concentrations against MPP+ and α-synuclein preformed fibrils (PFFs). Further, the levels of ROS and protein aggregation were significantly reduced at the optimal concentration (1 µM). Using two different mouse models, we gavaged C-6 at two different doses to the PD sign-displaying transgenic mice for 2 weeks and stereotaxically PFF-injected mice for 5 weeks. Our results demonstrated that both C-6-treated mouse models showed alleviated motor deficits in pole test, hindlimb clasping, crossbeam, rotarod, grooming, and nesting analyses. We also confirmed that the compound treatment reduced the levels of protein aggregation, along with phosphorylated-α-synuclein, in the striatum and ventral midbrain and further dopaminergic neuronal loss. Taken together, our results strongly suggest that NOX inhibition can be a potential therapeutic target for PD.
Development and Evolution of DNA-Dependent Protein Kinase Inhibitors toward Cancer Therapy
Authors: Matsumoto Yoshihisa
Published: April 2022

DNA double-strand break (DSB) is considered the most deleterious type of DNA damage, which is generated by ionizing radiation (IR) and a subset of anticancer drugs. DNA-dependent protein kinase (DNA-PK), which is composed of a DNA-PK catalytic subunit (DNA-PKcs) and Ku80-Ku70 heterodimer, acts as the molecular sensor for DSB and plays a pivotal role in DSB repair through non-homologous end joining (NHEJ). Cells deficient for DNA-PKcs show hypersensitivity to IR and several DNA-damaging agents. Cellular sensitivity to IR and DNA-damaging agents can be augmented by the inhibition of DNA-PK. A number of small molecules that inhibit DNA-PK have been developed. Here, the development and evolution of inhibitors targeting DNA-PK for cancer therapy is reviewed. Significant parts of the inhibitors were developed based on the structural similarity of DNA-PK to phosphatidylinositol 3-kinases (PI3Ks) and PI3K-related kinases (PIKKs), including Ataxia-telangiectasia mutated (ATM). Some of DNA-PK inhibitors, e.g., NU7026 and NU7441, have been used extensively in the studies for cellular function of DNA-PK. Recently developed inhibitors, e.g., M3814 and AZD7648, are in clinical trials and on the way to be utilized in cancer therapy in combination with radiotherapy and chemotherapy.
Cellular Computational Logic Using Toehold Switches
Authors: Choi Seungdo , Lee Geonhu , Kim Jongmin
Published: April 2022

The development of computational logic that carries programmable and predictable features is one of the key requirements for next-generation synthetic biological devices. Despite considerable progress, the construction of synthetic biological arithmetic logic units presents numerous challenges. In this paper, utilizing the unique advantages of RNA molecules in building complex logic circuits in the cellular environment, we demonstrate the RNA-only bitwise logical operation of XOR gates and basic arithmetic operations, including a half adder, a half subtractor, and a Feynman gate, in Escherichia coli. Specifically, de-novo-designed riboregulators, known as toehold switches, were concatenated to enhance the functionality of an OR gate, and a previously utilized antisense RNA strategy was further optimized to construct orthogonal NIMPLY gates. These optimized synthetic logic gates were able to be seamlessly integrated to achieve final arithmetic operations on small molecule inputs in cells. Toehold-switch-based ribocomputing devices may provide a fundamental basis for synthetic RNA-based arithmetic logic units or higher-order systems in cells.
The Interplay between Hydrogen Sulfide and Phytohormone Signaling Pathways under Challenging Environments
Authors: Khan Muhammad Saad Shoaib , Islam Faisal , Ye Yajin , Ashline Matthew , Wang Daowen , Zhao Biying , Fu Zheng Qing , Chen Jian
Published: April 2022

Hydrogen sulfide (H2S) serves as an important gaseous signaling molecule that is involved in intra- and intercellular signal transduction in plant–environment interactions. In plants, H2S is formed in sulfate/cysteine reduction pathways. The activation of endogenous H2S and its exogenous application has been found to be highly effective in ameliorating a wide variety of stress conditions in plants. The H2S interferes with the cellular redox regulatory network and prevents the degradation of proteins from oxidative stress via post-translational modifications (PTMs). H2S-mediated persulfidation allows the rapid response of proteins in signaling networks to environmental stimuli. In addition, regulatory crosstalk of H2S with other gaseous signals and plant growth regulators enable the activation of multiple signaling cascades that drive cellular adaptation. In this review, we summarize and discuss the current understanding of the molecular mechanisms of H2S-induced cellular adjustments and the interactions between H2S and various signaling pathways in plants, emphasizing the recent progress in our understanding of the effects of H2S on the PTMs of proteins. We also discuss future directions that would advance our understanding of H2S interactions to ultimately mitigate the impacts of environmental stresses in the plants.
Dissecting the Molecular Mechanisms Surrounding Post-COVID-19 Syndrome and Neurological Features
Authors: Mohamed Mohamed S. , Johansson Anton , Jonsson Jörgen , Schiöth Helgi B.
Published: April 2022

Many of the survivors of the novel coronavirus disease (COVID-19) are suffering from persistent symptoms, causing significant morbidity and decreasing their quality of life, termed “post-COVID-19 syndrome” or “long COVID”. Understanding the mechanisms surrounding PCS is vital to developing the diagnosis, biomarkers, and possible treatments. Here, we describe the prevalence and manifestations of PCS, and similarities with previous SARS epidemics. Furthermore, we look at the molecular mechanisms behind the neurological features of PCS, where we highlight important neural mechanisms that may potentially be involved and pharmacologically targeted, such as glutamate reuptake in astrocytes, the role of NMDA receptors and transporters (EAAT2), ROS signaling, astrogliosis triggered by NF-κB signaling, KNDy neurons, and hypothalamic networks involving Kiss1 (a ligand for the G-protein-coupled receptor 54 (GPR54)), among others. We highlight the possible role of reactive gliosis following SARS-CoV-2 CNS injury, as well as the potential role of the hypothalamus network in PCS manifestations.
A Novel Patent Ductus Arteriosus Severity Score to Predict Clinical Outcomes in Premature Neonates
Authors: Umapathi Krishna Kishore , Muller Brieann , Sosnowski Cyndi , Thavamani Aravind , Murphy Joshua , Awad Sawsan , Bokowski John W.
Published: April 2022

Background: Patent Ductus Arteriosus (PDA) in premature neonates has been associated with comorbidities including chronic lung disease (CLD), and death. However, the treatment of PDA remains controversial. There have been several echocardiographic variables previously used to determine the hemodynamic significance of PDA but their utility in early prediction of clinical outcomes is not well studied. Objective: The objective of our study was to evaluate the use of a severity scoring system incorporating markers of systemic under perfusion, pulmonary over perfusion and left ventricular (LV) function in predicting clinical outcomes in premature neonates. Methods: It is a single center prospective observational study involving newborns < 32 weeks’ gestation. An echocardiogram was done within seven days of life to measure variables previously known to predict severity of shunting in PDA including pulmonary perfusion index (PPI). Predictors of CLD/death were identified using multivariate logistic regression. A severity score was derived and its ability to predict clinical outcomes was tested using a receiver operating characteristic curve. Results: We studied 98 infants with a mean (SD) gestation of 28.9 ± 1.91 weeks and birth weight of 1228.06 ± 318.94 g, respectively. We identified five echocardiographic variables along with gestational age that was independently associated with the outcome variable (PPI, LV output, Superior Mesenteric Artery [SMA] Velocity Time Integral [VTI], Peak diastolic flow velocity in Pulmonary Vein [PV Vd], and reversal of flow in diastole in descending aorta [DFR]). The range of severity score was 0 (low risk) to 12 (high risk). A higher score was associated with the primary outcome variable of CLD/death (7.5 [1.2] vs. 3.6 [1.5], p < 0.001). Our severity score had an area under the curve of 0.97 (95% CI 0.93–0.99, p < 0.001) for predicting CLD/death. Conclusion: Our new PDA severity score of 5.5 has a sensitivity and specificity of 94% and 93%, and positive and negative predictive values of 94% and 93%, respectively.