bluuebunny/crossref_metadata_2025 huggingface dataset:
LAEA: A 2D LiDAR-Assisted UAV Exploration Algorithm for Unknown Environments
Authors: Hou Xiaolei , Pan Zheng , Lu Li , Wu Yuhang , Hu Jinwen , Lyu Yang , Zhao Chunhui
Published: March 2024

In UAV autonomous exploration, large frontier clusters are commonly associated with high information gain and are visited first. In contrast, small and isolated frontier clusters with fewer frontiers are associated with smaller information gain and are thus explored with low priority. However, these small and isolated frontier clusters are often in close proximity to UAVs and surrounded by explored areas, which could result in back-and-forth flights that lower exploration efficiency. This paper proposes LAEA, a LiDAR-assisted and depth camera-dominated UAV exploration algorithm that aims to improve UAV autonomous exploration efficiency. A hybrid map is obtained that characterizes rich environmental profile information in real time, enabling us to detect small and isolated frontier clusters that can lead to repeated visits to explored areas. An environmental information gain optimization strategy is incorporated such that frontier clusters with larger unexplored areas behind them, as well as small and isolated frontier clusters close to the UAV, are assigned higher weights to prioritize their visit order. An optimized flight trajectory is generated to cover unexplored frontier clusters in the immediate vicinity of the UAV while flying to the next target. A comprehensive comparison between the proposed algorithm and state-of-the-art algorithms was conducted via a simulation study, which showed that our algorithm exhibits superior exploration efficiency in various environments. Experiments were also carried out to verify the feasibility of the proposed approach in real-world scenarios.
Feedforward Enhanced Control System to Pursue Mathematical Competencies Achievement in Engineering Education
Authors: Mínguez-Aroca Francisco , Moll-López Santiago , Llobregat-Gómez Nuria , Roselló María-Dolores , Sánchez-Ruiz Luis M.
Published: March 2024

The learning process can be conceptualized as a dynamic system constantly evolving and adapting in response to a variety of stimuli and inputs. This paper presents a novel approach to competency-based assessment that integrates a dynamic assignment design of personalized activities with the aid of a 3D matrix array structure. This structure, named Topic, Activity, and Competency (TAC) cuboid, provides a detailed view of the student’s progress, enabling the design of tailored curricular strategies to facilitate the development of competencies. Personalized activities serve as control inputs that adapt to each learner’s state, driving a feedforward that promotes proactive improvement. An automation process becomes necessary to cope with the increased data volume from the TAC cuboid and the information complexity. The concept model registers TAC cuboid information, enabling a dynamic analysis to forward an adequate workload when required to facilitate students’ achievement of their expected mathematical competencies. Additionally, the paper discusses the initial application of these models in an educational role-playing game, demonstrating their effectiveness in a gamified learning environment.
Does the Understanding of Managing Variables among Pre-Service Early Childhood Teachers Correspond to Distinct Teaching Methods in Their Future Careers?
Authors: Zoupidis Anastasios , Tselfes Vassilis , Kariotoglou Petros
Published: March 2024

This study aims to examine the views of pre-service early childhood student teachers (referred to as student teachers) regarding the potential impact of an inquiry-based science course on their intention to utilize inquiry-based learning approaches, such as the Control of Variables Strategy (CVS) method. The study comprised a preliminary phase (N = 17) and a primary phase (N = 81). The participants’ level of understanding of the inquiry-based method was assessed using an open- and semi-open questionnaire. A 34-item Likert-type questionnaire was created using the Theory of Planned Behavior in the preliminary phase of the study and utilized in the primary phase to examine student teachers’ intentions to include the CVS technique into their lessons. The results showed student teachers’ strong intention to implement inquiry-based learning strategies, being supported by their positive estimations of managing the CVS method engagement in the classroom. However, their estimations of the significant others’ opinions and the personal gain–loss balance expected from engaging with the CVS method significantly hindered their intention. Most interestingly, the results of the study suggest that the various levels of comprehension of the CVS method attained by student teachers are connected to the adoption of distinct approaches in their future teaching endeavors. In light of these results, we discuss several teaching implications.
Multi-Layered Satellite Communications Systems for Ultra-High Availability and Resilience
Authors: Höyhtyä Marko , Anttonen Antti , Majanen Mikko , Yastrebova-Castillo Anastasia , Varga Mihaly , Lodigiani Luca , Corici Marius , Zope Hemant
Published: March 2024

Satellite communications systems provide a means to connect people and devices in hard-to-reach locations. Traditional geostationary orbit (GEO) satellite systems and low Earth orbit (LEO) constellations, having their own strengths and weaknesses, have been used as separate systems serving different markets and customers. In this article, we analyze how satellite systems in different orbits could be integrated together and used as a multi-layer satellite system (MLSS) to improve communication services. The optimization concerns combining the strengths of different layers that include a larger coverage area as one moves up by each layer of altitude and a shorter delay as one moves down by each layer of altitude. We review the current literature and market estimates and use the information to provide a thorough assessment of the economic, regulatory, and technological enablers of the MLSS. We define the MLSS concept and the architecture and describe our testbed and the simulation tools used as a comprehensive engineering proof-of-concept. The validation results confirm that the MLSS approach can intelligently exploit the smaller jitter of GEO and shorter delay of LEO connections, and it can increase the availability and resilience of communication services. As a main conclusion, we can say that multi-layered networks and the integration of satellite and terrestrial segments seem very promising candidates for future 6G systems.
Study on Landslide Displacement Prediction Considering Inducement under Composite Model Optimization
Authors: Ye Shun , Liu Yu , Xie Kai , Wen Chang , Tian Hong-Ling , He Jian-Biao , Zhang Wei
Published: March 2024

The precise extraction of displacement time series for complex landslides poses significant challenges, and conventional landslide prediction models often overlook the deformation impacts of displacement triggers. To address this, we introduce a novel composite model tailored for predicting landslide displacement. This model employs Variational Mode Decomposition (VMD) to isolate each displacement component, with optimization achieved through the groupwise coupling algorithm. Subsequently, Grey correlation analysis (GRA) is applied to quantitatively assess the dynamic correlations between various triggering factors and landslide displacement. This analysis informs the construction of a feature set predicated on these correlation factors. Integrating the time-series VMD module into the standard Transformer architecture facilitates the prediction of landslide displacement. This integration allows for the extraction of critical time-evolution features associated with the displacement components. Ultimately, the predicted displacements are aggregated and reconstructed. We validate our model using the Bazimen landslide case study, analyzing displacement monitoring data from 1 January 2007, to 31 December 2012. The values of the root mean square error and the mean absolute percentage error were 1.86 and 4.85, respectively. This model offers a more nuanced understanding of the multifaceted causes and evolutionary dynamics underpinning landslide displacement and deformation, thereby markedly enhancing prediction accuracy.
Integrated Active and Reactive Power Control Methods for Distributed Energy Resources in Distribution Systems for Enhancing Hosting Capacity
Authors: Trinh Phi-Hai , Chung Il-Yop
Published: March 2024

Recently, there has been a significant increase in the integration of distributed energy resources (DERs) such as small-scale photovoltaic systems and wind turbines in power distribution systems. When the aggregated outputs of DERs are combined, excessive reverse current may occur in distribution lines, leading to overvoltage issues and exceeding thermal limits of the distribution lines. To address these issues, it is necessary to limit the output of DERs to a certain level, which results in constraining the hosting capacity of DERs in the distribution system. In this paper, coordination control methodologies of DERs are developed and executed to mitigate the overvoltage and overcurrent induced by DERs, thereby increasing the hosting capacity for DERs of the distribution system. This paper proposes three coordinated approaches of active and reactive power control of DERs, namely Var Precedence, Watt Precedence, and Integrated Watt and Var Control. The Var and Watt Precedence prioritizes reactive power for voltage (Q–V) and active power for current (P–I) to address network congestion, thereby enhancing hosting capacity. Conversely, the Integrated Var and Watt Precedence propose a novel algorithm that combines four control indices (Q–V, P–V, Q–I, and P–I) to solve network problems while maximizing hosting capacity. The three proposed methods are based on the sensitivity analysis of voltage and current to the active and reactive power outputs at the DER installation locations on the distribution lines, aiming to minimize DER active power curtailment. Each sensitivity is derived from linearized power equations at the operating points of the distribution system. To minimize the computation burden of iterative computation, each proposed method decouples active and reactive power and proceeds with sequential control in its own unique way, iteratively determining the precise output control of distributed power sources to reduce linearization errors. The three proposed algorithms are verified via case studies, evaluating their performance compared to conventional approaches. The case studies exhibit superior control effectiveness of the proposed DER power control methods compared to conventional methods when issues such as overvoltage and overcurrent occur simultaneously in the distribution line so that the DER hosting capacity of the system can be improved.
Teaming up Radio and Sub-mm/FIR Observations to Probe Dusty Star-Forming Galaxies
Authors: Behiri Meriem , Giulietti Marika , Galluzzi Vincenzo , Lapi Andrea , Liuzzo Elisabetta , Massardi Marcella
Published: March 2024

In this paper, we investigate the benefits of teaming up data from the radio to the far-infrared (FIR) regime for the characterization of dusty star-forming galaxies (DSFGs). These galaxies are thought to be the star-forming progenitors of local massive quiescent galaxies and to play a pivotal role in the reconstruction of the cosmic star formation rate density up to high redshift. Due to their dust-enshrouded nature, DSFGs are often invisible in the near-infrared/optical/UV bands. Therefore, they necessitate observations at longer wavelengths, primarily the FIR band, where dust emission occurs, and the radio band, which is not affected by dust absorption. Combining data from these two spectral windows makes it possible to characterize even the dustiest objects, enabling the retrieval of information about their age, dust temperature, and star-formation status, and facilitates the differentiation between various galaxy populations that evolve throughout cosmic history. Despite the detection of faint radio sources being a challenging task, this study demonstrates that an effective strategy to build statistically relevant samples of DSFGs would be reaching deep sensitivities in the radio band, even restricted to smaller areas, and then combining these radio observations with FIR/submm data. Additionally, this paper quantifies the improvement in the spectral energy distribution (SED) reconstruction of DSFGs by incorporating ALMA band measurements, in particular, in its upgraded status thanks to the anticipated Wideband Sensitivity Upgrade.
Resequencing Analyses Revealed Genetic Diversity and Selection Signatures during Rabbit Breeding and Improvement
Authors: Xie Kerui , Ning Chao , Yang Aiguo , Zhang Qin , Wang Dan , Fan Xinzhong
Published: March 2024

Domestication has shaped the diverse characteristics of rabbits, including coat color, fur structure, body size, and various physiological traits. Utilizing whole-genome resequencing (DNBSEQ-T7), we analyzed the genetic diversity, population structure, and genomic selection across 180 rabbits from 17 distinct breeds to uncover the genetic basis of these traits. We conducted whole-genome sequencing on 17 rabbit breeds, identifying 17,430,184 high-quality SNPs and analyzing genomic diversity, patterns of genomic variation, population structure, and selection signatures related to coat color, coat structure, long hair, body size, reproductive capacity, and disease resistance. Through PCA and NJ tree analyses, distinct clusters emerged among Chinese indigenous rabbits, suggesting varied origins and domestication histories. Selective sweep testing pinpointed regions and genes linked to domestication and key morphological and economic traits, including those affecting coat color (TYR, ASIP), structure (LIPH), body size (INSIG2, GLI3), fertility (EDNRA, SRD5A2), heat stress adaptation (PLCB1), and immune response (SEC31A, CD86, LAP3). Our study identified key genomic signatures of selection related to traits such as coat color, fur structure, body size, and fertility; these findings highlight the genetic basis underlying phenotypic diversification in rabbits and have implications for breeding programs aiming to improve productive, reproductive, and adaptive traits. The detected genomic signatures of selection also provide insights into rabbit domestication and can aid conservation efforts for indigenous breeds.
Language and Communication Interventions in People with Alzheimer’s Disease: A Systematic Review
Authors: Dimitriou Nefeli K. , Nousia Anastasia , Georgopoulou Eleni-Nefeli , Martzoukou Maria , Liampas Ioannis , Dardiotis Efthimios , Nasios Grigorios
Published: March 2024

Although language impairment is frequently observed in patients with Alzheimer’s disease (pwAD), targeted language rehabilitation is often overlooked. The present study reviews published evidence on the impact of language training, either alone or in combination with cognitive training, on cognitive outcomes in pwAD. A systematic search of PubMed, Google Scholar, and Cochrane was carried out, including studies published from inception to November 2023. A total of eight research articles (four randomized controlled trials and four observational studies) met the inclusion criteria: six assessed language training combined with cognitive training and two evaluated language rehabilitation alone. Regarding language and non-language (mainly memory, attention, and executive functions) outcomes, there was a consensus among studies that language rehabilitation (alone or in combination with cognitive training) yields positive results. Some of the articles also explored the impact on patients’ and their caregivers’ quality of life, with all but one showing improvement. Consequently, the combination of language and cognitive training leads to improvements across various cognitive domains. However, limited evidence supports the value of sole language rehabilitation. This conclusion is influenced by heterogeneity among studies (different types and duration of interventions, small participant sets, various assessment tools), and, thus, further research is warranted.
The Development of a Multi-Modal Cancer Rehabilitation (Including Prehabilitation) Service in Sheffield, UK: Designing the Active Together Service
Authors: Humphreys Liam , Myers Anna , Frith Gabriella , Thelwell Michael , Pickering Katie , Mills Gary H. , Kerr Karen , Fisher Patricia , Kidder John , Keen Carol , Hodson Suzanne , Phillips Gail , Smith Rachel , Evans Laura , Thornton Sarah , Dale Emma , Maxwell Louise , Greenfield Diana M. , Copeland Robert
Published: March 2024

Cancer patients undergoing major interventions face numerous challenges, including the adverse effects of cancer and the side effects of treatment. Cancer rehabilitation is vital in ensuring cancer patients have the support they need to maximise treatment outcomes and minimise treatment-related side effects and symptoms. The Active Together service is a multi-modal rehabilitation service designed to address critical support gaps for cancer patients. The service is located and provided in Sheffield, UK, an area with higher cancer incidence and mortality rates than the national average. The service aligns with local and regional cancer care objectives and aims to improve the clinical and quality-of-life outcomes of cancer patients by using lifestyle behaviour-change techniques to address their physical, nutritional, and psychological needs. This paper describes the design and initial implementation of the Active Together service, highlighting its potential to support and benefit cancer patients.
Mycorrhizal Symbiosis Enhances P Uptake and Indole-3-Acetic Acid Accumulation to Improve Root Morphology in Different Citrus Genotypes
Authors: Liu Chun-Yan , Guo Xiao-Niu , Dai Feng-Jun , Wu Qiang-Sheng
Published: March 2024

Arbuscular mycorrhizal fungi (AMF) are known to enhance plant growth via stimulation of root system development. However, the extent of their effects and underlying mechanisms across different citrus genotypes remain to be fully elucidated. This study investigates the impact of Funneliformis mosseae (F. mosseae) inoculation on plant growth performance, root morphology, phosphorus (P), and indole-3-acetic acid (IAA) concentrations, as well as the expression of related synthesis and transporter genes in three citrus genotypes: red tangerine (Citrus tangerine ex. Tanaka), kumquat (Fortunella margarita L. Swingle), and fragrant citrus (Citrus junos Sieb. ex. Tanaka). Following 12 weeks of inoculation, significant improvements were observed in plant height, shoot and root biomass, total root length, average root diameter, second-order lateral root development, root hair density, and root hair length across all genotypes. Additionally, F. mosseae inoculation significantly increased root P and IAA concentrations in the three citrus genotypes. Notably, phosphatase activity was enhanced in F. margarita but reduced in C. tangerine and C. junos following inoculation. Gene expression analysis revealed a universal upregulation of the P transporter gene PT5, whereas expressions of the auxin synthesis gene YUC2, transporter gene LAX2, and phosphatase gene PAP1 were commonly downregulated. Specific to genotypes, expressions of YUC5, LAX5, PIN2, PIN3, PIN6, and expansin genes EXPA2 and EXPA4 were significantly upregulated in C. tangerine but downregulated in F. margarita and C. junos. Principal component analysis and correlation assessments highlighted a strong positive association between P concentration, P and auxin synthesis, and transporter gene expressions with most root morphology traits, except for root average diameter. Conversely, IAA content and phosphatase activities were negatively correlated with these root traits. These findings suggest that F. mosseae colonization notably enhances plant growth and root system architecture in citrus genotypes via modifications in P transport and IAA accumulation, indicating a complex interplay between mycorrhizal symbiosis and host plant physiology.
An Integrated Duranton and Overman Index and Local Duranton and Overman Index Framework for Industrial Spatial Agglomeration Pattern Analysis
Authors: Huang Yupu , Zhuo Li , Cao Jingjing
Published: March 2024

Accurately measuring industrial spatial agglomeration patterns is crucial for promoting regional economic development. However, few studies have considered both agglomeration degrees and cluster locations of industries. Moreover, the traditional multi-scale cluster location mining (MCLM) method still has limitations in terms of accuracy, parameter setting, calculation efficiency, etc. This study proposes a new framework for analyzing industrial spatial agglomeration patterns, which uses the Duranton and Overman (DO) index for estimating agglomeration degrees and a newly developed local DO (LDO) index for mining cluster locations. The MCLM-LDO method was proposed by incorporating the LDO index into the MCLM method, and it was validated via comparisons with three baseline methods based on two synthetic datasets. The results proved that the MCLM-LDO method can achieve accuracies of 0.945 and 1 with computational times of 0.15 s and 0.11 s on two datasets, which are superior to existing MCLM methods. The proposed framework was further applied to analyze the spatial agglomeration patterns of the industry of computer, communication, and other electronic equipment manufacturing in Guangdong Province, China. The results showed that the framework gives a more holistic perspective of spatial agglomeration patterns, which can serve as more meaningful references for industrial sustainable development.
The Role of Index of Microcirculatory Resistance in Left Anterior Descending Artery ST Segment Elevation Myocardial Infarction Patients after Primary Percutaneous Coronary Intervention
Authors: Choi Seong Huan , Ahn Sung Gyun , Yoon Myeong Ho , Seo Kyoung-Woo , Lee Ki-Jeung , Kwon Sung Woo , Park Sang-Don , Woo Seong-Ill
Published: March 2024

Background: Our aim was to assess the relationship of the index of microvascular resistance (IMR) in left anterior descending (LAD) artery involved STEMI patients. Methods: Data of 316 STEMI patients who had undergone primary percutaneous coronary intervention (PCI) were collected from three cardiovascular centers from 2005 to 2015. In total, 246 patients with LAD STEMI were enrolled for IMR evaluation. Patients were divided into two groups respective of the cut-off IMR value of 30. All-cause mortality, left ventricular function, improvement of systolic function, and cardiac biomarkers were analyzed and compared. Results: A total of 246 patients were enrolled. The number of patients in the IMR above 30 group was 93 and below 30 was 153. The mean ages for each group were 57.91 ± 11.99 and 54 ± 10.63, respectively. The peak creatinine kinase (CK) (3936.85 ± 2827.32 IU/L vs. 2218.08 ± 2310.41 IU/L, p < 0.001) and CKmb (336.15 ± 195.08 mg/mL vs. 231.53 ± 179.53 mg/mL, p < 0.001) levels were higher for an IMR above the 30 group. The left ventricular ejection fraction (LVEF) (44.57 ± 6.685% vs. 47.35 ± 8.17%, p = 0.006) and improvement of LVEF (2.81 ± 7.135% vs. 5.88 ± 7.65%, p = 0.004) was lower in the IMR above 30 group. All-cause mortality (7.5% vs. 1.3%, p = 0.012) was higher in the IMR above 30 group, and a Cox regression analysis showed that an IMR above 30 was a poor prognostic factor regarding all-cause mortality (HR: 5.151, 95% CI 1.062–24.987, p = 0.042) even after adjusting for classical clinical risk factors. Conclusions: An elevated IMR value represented larger infarct size, more severe LV dysfunction, and higher mortality in LAD STEMI patients after successful PCI.
Circulation and Stratification Changes in a Hypersaline Estuary Due to Mean Sea Level Rise
Authors: Taebi Soheila , Pattiaratchi Charitha , Haigh Ivan , Kendrick Gary
Published: March 2024

Hypersaline Hamelin Pool, with mean salinity >65, is located in Shark Bay, Western Australia. The high salinity has reduced its biodiversity, but it is home to a diverse assemblage of modern marine stromatolites. The limited exchange of water between Hamelin Pool and the rest of Shark Bay, due to the presence of the shallow Faure Sill together with high evaporation and low rainfall-runoff have resulted in a hypersaline environment. With climate-change-induced mean sea level rise (MSLR), hydrodynamic processes that maintain the hypersaline environment may be affected and are the focus of this paper. Oceanographic observations, together with hydrodynamic model results, were used to examine the hydrodynamic processes under present and future MSLR scenarios. A large attenuation in the tidal range, changes in the tidal characteristics, and current speeds together with a strong salinity gradient were observed across the Faure Sill under present-day conditions. Under an MSLR scenario of 1 m, the tidal amplitude decreased by up to 10% to the north, whilst to the south, the tidal range increased by up to 15%. Regions of strong vertical stratification were present on both sides of the Faure Sill. The simulations indicated that, under MSLR scenarios, these regions expanded in area and exhibited higher levels of vertical stratification. The salt flux across the Faure Sill was maintained as a diffusive process under MSLR scenarios.
The Impact of Essential Amino Acids on the Gut Microbiota of Broiler Chickens
Authors: Taylor-Bowden Thyneice , Bhogoju Sarayu , Khwatenge Collins N. , Nahashon Samuel N.
Published: March 2024

The research involving the beneficial aspects of amino acids being added to poultry feed pertaining to performance, growth, feed intake, and feed conversion ratio is extensive. Yet currently the effects of amino acids on the gut microbiota aren’t fully understood nor have there been many studies executed in poultry to explain the relationship between amino acids and the gut microbiota. The overall outcome of health has been linked to bird gut health due to the functionality of gastrointestinal tract (GIT) for digestion/absorption of nutrients as well as immune response. These essential functions of the GI are greatly driven by the resident microbiota which produce metabolites such as butyrate, propionate, and acetate, providing the microbiota a suitable and thrive driven environment. Feed, age, the use of feed additives and pathogenic infections are the main factors that have an effect on the microbial community within the GIT. Changes in these factors may have potential effects on the gut microbiota in the chicken intestine which in turn may have an influence on health essentially affecting growth, feed intake, and feed conversion ratio. This review will highlight limited research studies that investigated the possible role of amino acids in the gut microbiota composition of poultry.
Pathogenicity of Aeromonas veronii Isolated from Diseased Macrobrachium rosenbergii and Host Immune-Related Gene Expression Profiles
Authors: Gao Xiaojian , Chen Zhen , Zhang Zirui , Qian Qieqi , Chen Anting , Qin Lijie , Tang Xinzhe , Jiang Qun , Zhang Xiaojun
Published: March 2024

Aeromonas veronii is widespread in aquatic environments and is responsible for infecting various aquatic animals. In this study, a dominant strain was isolated from the hepatopancreas of diseased Macrobrachium rosenbergii and was named JDM1-1. According to its morphological, physiological, and biochemical characteristics and molecular identification, isolate JDM1-1 was identified as A. veronii. The results of artificial challenge showed isolate JDM1-1 had high pathogenicity to M. rosenbergii with an LD50 value of 8.35 × 105 CFU/mL during the challenge test. Histopathological analysis revealed severe damage in the hepatopancreas and gills of the diseased prawns, characterized by the enlargement of the hepatic tubule lumen and gaps between the tubules as well as clubbing and degeneration observed at the distal end of the gill filament. Eight virulence-related genes, namely aer, ompA, lip, tapA, hlyA, flgA, flgM, and flgN, were screened by PCR assay. In addition, virulence factor detection showed that the JDM1-1 isolate produced lipase, lecithinase, gelatinase, and hemolysin. Furthermore, the mRNA expression profiles of immune-related genes of M. rosenbergii following A. veronii infection, including ALF1, ALF2, Crustin, C-lectin, and Lysozyme, were assessed, and the results revealed a significant upregulation in the hepatopancreas and intestines at different hours post infection. This study demonstrates that A. veronii is a causative agent associated with massive die-offs of M. rosenbergii and contributes valuable insights into the pathogenesis and host defense mechanisms of A. veronii invasion.
Genomic and Phenotypic Analysis of Salmonella enterica Bacteriophages Identifies Two Novel Phage Species
Authors: Bhandare Sudhakar , Lawal Opeyemi U. , Colavecchio Anna , Cadieux Brigitte , Zahirovich-Jovich Yella , Zhong Zeyan , Tompkins Elizabeth , Amitrano Margot , Kukavica-Ibrulj Irena , Boyle Brian , Wang Siyun , Levesque Roger C. , Delaquis Pascal , Danyluk Michelle , Goodridge Lawrence
Published: March 2024

Bacteriophages (phages) are potential alternatives to chemical antimicrobials against pathogens of public health significance. Understanding the diversity and host specificity of phages is important for developing effective phage biocontrol approaches. Here, we assessed the host range, morphology, and genetic diversity of eight Salmonella enterica phages isolated from a wastewater treatment plant. The host range analysis revealed that six out of eight phages lysed more than 81% of the 43 Salmonella enterica isolates tested. The genomic sequences of all phages were determined. Whole-genome sequencing (WGS) data revealed that phage genome sizes ranged from 41 to 114 kb, with GC contents between 39.9 and 50.0%. Two of the phages SB13 and SB28 represent new species, Epseptimavirus SB13 and genera Macdonaldcampvirus, respectively, as designated by the International Committee for the Taxonomy of Viruses (ICTV) using genome-based taxonomic classification. One phage (SB18) belonged to the Myoviridae morphotype while the remaining phages belonged to the Siphoviridae morphotype. The gene content analyses showed that none of the phages possessed virulence, toxin, antibiotic resistance, type I–VI toxin–antitoxin modules, or lysogeny genes. Three (SB3, SB15, and SB18) out of the eight phages possessed tailspike proteins. Whole-genome-based phylogeny of the eight phages with their 113 homologs revealed three clusters A, B, and C and seven subclusters (A1, A2, A3, B1, B2, C1, and C2). While cluster C1 phages were predominantly isolated from animal sources, cluster B contained phages from both wastewater and animal sources. The broad host range of these phages highlights their potential use for controlling the presence of S. enterica in foods.
Comprehensive Genomics Investigation of Neboviruses Reveals Distinct Codon Usage Patterns and Host Specificity
Authors: Kaushik Rahul , Kumar Naveen , Yadav Pragya , Sircar Shubhankar , Shete-Aich Anita , Singh Ankur , Tomar Shailly , Launey Thomas , Malik Yashpal Singh
Published: March 2024

Neboviruses (NeVs) from the Caliciviridae family have been linked to enteric diseases in bovines and have been detected worldwide. As viruses rely entirely on the cellular machinery of the host for replication, their ability to thrive in a specific host is greatly impacted by the specific codon usage preferences. Here, we systematically analyzed the codon usage bias in NeVs to explore the genetic and evolutionary patterns. Relative Synonymous Codon Usage and Effective Number of Codon analyses indicated a marginally lower codon usage bias in NeVs, predominantly influenced by the nucleotide compositional constraints. Nonetheless, NeVs showed a higher codon usage bias for codons containing G/C at the third codon position. The neutrality plot analysis revealed natural selection as the primary factor that shaped the codon usage bias in both the VP1 (82%) and VP2 (57%) genes of NeVs. Furthermore, the NeVs showed a highly comparable codon usage pattern to bovines, as reflected through Codon Adaptation Index and Relative Codon Deoptimization Index analyses. Notably, yak NeVs showed considerably different nucleotide compositional constraints and mutational pressure compared to bovine NeVs, which appear to be predominantly host-driven. This study sheds light on the genetic mechanism driving NeVs’ adaptability, evolution, and fitness to their host species.
The Incidence and Risk Factors for Enterotoxigenic E. coli Diarrheal Disease in Children under Three Years Old in Lusaka, Zambia
Authors: Sukwa Nsofwa , Bosomprah Samuel , Somwe Paul , Muyoyeta Monde , Mwape Kapambwe , Chibesa Kennedy , Luchen Charlie Chaluma , Silwamba Suwilanji , Mulenga Bavin , Munyinda Masiliso , Muzazu Seke , Chirwa Masuzyo , Chibuye Mwelwa , Simuyandi Michelo , Chilengi Roma , Svennerholm Ann-Mari
Published: March 2024

This study aimed to estimate the incidence and risk factors for Enterotoxigenic Escherichia coli (ETEC) diarrhea. This was a prospective cohort study of children recruited in a household census. Children were enrolled if they were 36 months or below. A total of 6828 children were followed up passively for 12 months to detect episodes of ETEC diarrhea. Diarrheal stool samples were tested for ETEC using colony polymerase chain reaction (cPCR). Among the 6828 eligible children enrolled, a total of 1110 presented with at least one episode of diarrhea. The overall incidence of ETEC diarrhea was estimated as 2.47 (95% confidence interval (CI): 2.10–2.92) episodes per 100 child years. Children who were HIV-positive (adjusted Hazard ratio (aHR) = 2.14, 95% CI: 1.14 to 3.99; p = 0.017) and those whose source of drinking water was public tap/borehole/well (aHR = 2.45, 95% CI: 1.48 to 4.06; p < 0.002) were at increased risk of ETEC diarrhea. This study found that children whose mothers have at least senior secondary school education (aHR = 0.49, 95% CI: 0.29 to 0.83; p = 0.008) were at decreased risk of ETEC diarrhea. Our study emphasizes the need for integrated public health strategies focusing on water supply improvement, healthcare for persons living with HIV, and maternal education.
Coal-Derived Humic Substances: Insight into Chemical Structure Parameters and Biomedical Properties
Authors: Zykova Maria V. , Bratishko Kristina A. , Buyko Evgeny E. , Azarkina Lyudmila A. , Ivanov Vladimir V. , Mihalyov Dmitrii A. , Trofimova Evgeniya S. , Danilets Marina G. , Ligacheva Anastasia A. , Konstantinov Andrey I. , Ufandeev Alexander A. , Rabtsevich Evgenia S. , Drygunova Larisa A. , Zima Anastasia P. , Bashirov Sergey R. , Udut Elena V. , Belousov Mikhail V.
Published: March 2024

An investigation was carried out on humic substances (HSs) isolated from the coal of the Kansk-Achinsk basin (Krasnoyarsk Territory, Russia). The coal HSs demonstrate the main parameters of molecular structure inherent to this class of natural compounds. An assessment was performed for the chemical, microbiological, and pharmacological safety parameters, as well as the biological efficacy. The HS sample meets the safety requirements in microbiological purity, toxic metals content (lead, cadmium, mercury, arsenic), and radionuclides. The presence of 11 essential elements was determined. The absence of general, systemic toxicity, cytotoxicity, and allergenic properties was demonstrated. The coal HS sample was classified as a Class V hazard (low danger substances). High antioxidant and antiradical activities and immunotropic and cytoprotective properties were identified. The ability of the HS to inhibit hydroxyl radicals and superoxide anion radicals was revealed. Pronounced actoprotective and nootropic activities were also demonstrated in vivo. Intragastric administration of the HS sample resulted in the improvement of physical parameters in mice as assessed by the “swim exhaustion” test. Furthermore, intragastric administration in mice with cholinergic dysfunction led to a higher ability of animals with scopolamine-induced amnesia to form conditioned reflexes. These findings suggest that the studied HS sample is a safe and effective natural substance, making it suitable for use as a dietary bioactive supplement.
Chlorpromazine–Polypyrrole Drug Delivery System Tailored for Neurological Application
Authors: Krawczyk Sara , Golba Sylwia , Neves Cristina , Tedim João
Published: March 2024

Nowadays, drug delivery systems (DDSs) are gaining more and more attention. Conducting polymers (CPs) are efficiently used for DDS construction as such systems can be used in therapy. In this research, a well-known CP, polypyrrole (PPy), was synthesized in the presence of the polysaccharide heparin (HEP) and chlorpromazine (CPZ) using sodium dodecyl sulfate (SDS) as electrolyte on a steel substrate. The obtained results demonstrate the successful incorporation of CPZ and HEP into the polymer matrix, with the deposited films maintaining stable electrochemical parameters across multiple doping/dedoping cycles. Surface roughness, estimated via AFM analysis, revealed a correlation with layer thickness—decreasing for thinner layers and increasing for thicker ones. Moreover, SEM images revealed a change in the morphology of PPy films when PPy is electropolymerized in the presence of CPZ and HEP, while FTIR confirmed the presence of CPZ and HEP within PPy. Due to its lower molecular mass compared to HEP, CPZ was readily integrated into the thin polymer matrix during deposition, with diffusion being unimpeded, as opposed to films with greater thickness. Finally, the resulting system exhibited the ability to release CPZ, enabling a dosing range of 10 mg to 20 mg per day, effectively covering the therapeutic concentration range.
Exploration of the Antibacterial and Anti-Inflammatory Activity of a Novel Antimicrobial Peptide Brevinin-1BW
Authors: Chen Zhizhi , Wang Lei , He Dongxia , Liu Qi , Han Qinqin , Zhang Jinyang , Zhang A-Mei , Song Yuzhu
Published: March 2024

Antibiotic resistance has emerged as a grave threat to global public health, leading to an increasing number of treatment failures. Antimicrobial peptides (AMPs) are widely regarded as potential substitutes for traditional antibiotics since they are less likely to induce resistance when used. A novel AMP named Brevinin-1BW (FLPLLAGLAASFLPTIFCKISRKC) was obtained by the Research Center of Molecular Medicine of Yunnan Province from the skin of the Pelophylax nigromaculatus. Brevinia-1BW had effective inhibitory effects on Gram-positive bacteria, with a minimum inhibitory concentration (MIC) of 3.125 μg/mL against Enterococcus faecalis (ATCC 29212) and 6.25 μg/mL against both Staphylococcus aureus (ATCC 25923) and multidrug-resistant Staphylococcus aureus (ATCC 29213) but had weaker inhibitory effects on Gram-negative bacteria, with a MIC of ≥100 μg/mL. Studies using scanning electron microscopy (SEM) and flow cytometry have revealed that it exerts its antibacterial activity by disrupting bacterial membranes. Additionally, it possesses strong biofilm inhibitory and eradication activities as well as significant lipopolysaccharide (LPS)-binding activity. Furthermore, Brevinin-1BW has shown a significant anti-inflammatory effect in LPS-treated RAW264.7 cells. In conclusion, Brevinin-1BW is anticipated to be a promising clinical agent with potent anti-Gram-positive bacterial and anti-inflammatory properties.
Association between Multiple Trace Elements, Executive Function, and Cognitive Impairment with No Dementia in Older Adults
Authors: Ryoo Seung-Woo , Choi Baek-Yong , Son Seok-Yoon , Oh Kun-Hee , Min Jin-Young , Min Kyoung-Bok
Published: March 2024

Many studies suggest a significant association between individual essential trace elements (ETEs) and cognitive impairment in older adults, but evidence of the synchronized effect of multiple ETEs on cognitive function is lacking. We investigated the association between multiple ETEs, cognitive impairment with no dementia (CIND), and executive function in older Korean adults, using the Bayesian kernel machine regression (BKMR) model. Three hundred and thirty-six older adults were included as the study population and classified as the CIND and control groups. Blood manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), and molybdenum (Mo) were measured as relevant ETEs. The frontal/executive tests included digit symbol coding (DSC), the Korean color word Stroop test (K-CWST), a controlled oral word association test (COWAT), and a trial-making test (TMT). Overall, the BKMR showed a negative association between multiple ETEs and the odds of CIND. Mn was designated as the most dominant element associated with the CIND (PIP = 0.6184), with a U-shaped relationship. Cu and Se levels were positively associated with the K-CWST percentiles (β = 31.78; 95% CI: 13.51, 50.06) and DSC percentiles (β = 25.10; 95% CI: 7.66, 42.53), respectively. Our results suggest that exposure to multiple ETEs may be linked to a protective mechanism against cognitive impairment in older adults.
Prebiotic Strategies to Manage Lactose Intolerance Symptoms
Authors: Angima Gloria , Qu Yunyao , Park Si Hong , Dallas David C.
Published: March 2024

Lactose intolerance, which affects about 65–75% of the world’s population, is caused by a genetic post-weaning deficiency of lactase, the enzyme required to digest the milk sugar lactose, called lactase non-persistence. Symptoms of lactose intolerance include abdominal pain, bloating and diarrhea. Genetic variations, namely lactase persistence, allow some individuals to metabolize lactose effectively post-weaning, a trait thought to be an evolutionary adaptation to dairy consumption. Although lactase non-persistence cannot be altered by diet, prebiotic strategies, including the consumption of galactooligosaccharides (GOSs) and possibly low levels of lactose itself, may shift the microbiome and mitigate symptoms of lactose consumption. This review discusses the etiology of lactose intolerance and the efficacy of prebiotic approaches like GOSs and low-dose lactose in symptom management.
The Role of Acyl-CoA Synthetase 1 in Bioactive Lipid Accumulation and the Development of Hepatic Insulin Resistance
Authors: Zabielski Piotr , Imierska Monika , Roszczyc-Owsiejczuk Kamila , Kuźmicki Mariusz , Rogalski Paweł , Daniluk Jarosław , Błachnio-Zabielska Agnieszka U.
Published: March 2024

The liver plays a crucial role in glucose metabolism. Obesity and a diet rich in fats (HFD) contribute to the accumulation of intracellular lipids. The aim of the study was to explore the involvement of acyl-CoA synthetase 1 (ACSL1) in bioactive lipid accumulation and the induction of liver insulin resistance (InsR) in animals fed an HFD. The experiments were performed on male C57BL/6 mice divided into the following experimental groups: 1. Animals fed a control diet; 2. animals fed HFD; and 3. HFD-fed animals with the hepatic ACSL1 gene silenced through a hydrodynamic gene delivery technique. Long-chain acyl-CoAs, sphingolipids, and diacylglycerols were measured by LC/MS/MS. Glycogen was measured by means of a commercially available kit. The protein expression and phosphorylation state of the insulin pathway was estimated by Western blot. HFD-fed mice developed InsR, manifested as an increase in fasting blood glucose levels (202.5 mg/dL vs. 130.5 mg/dL in the control group) and inhibition of the insulin pathway, which resulted in an increase in the rate of gluconeogenesis (0.420 vs. 0.208 in the control group) and a decrease in the hepatic glycogen content (1.17 μg/mg vs. 2.32 μg/mg in the control group). Hepatic ACSL1 silencing resulted in decreased lipid content and improved insulin sensitivity, accounting for the decreased rate of gluconeogenesis (0.348 vs. 0.420 in HFD(+/+)) and the increased glycogen content (4.3 μg/mg vs. 1.17 μg/mg in HFD(+/+)). The elevation of gluconeogenesis and the decrease in glycogenesis in the hepatic tissue of HFD-fed mice resulted from cellular lipid accumulation. Inhibition of lipid synthesis through silencing ACSL1 alleviated HFD-induced hepatic InsR.
The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria
Authors: Zhu Na , Liu Rui , Xu Meihong , Li Yong
Published: March 2024

Extensive in vivo investigations have demonstrated the antioxidant properties of fish collagen oligopeptides (FCOPs). One of the main causes of aging and chronic non-communicable diseases is oxidative stress. Therefore, FCOPs have a broad range of applications in illness prevention and delaying aging from the standpoint of the “food is medicine” theory. However, the mechanisms that underpin the antioxidant activity of FCOPs are not completely understood. The specific objective of this essay was to investigate the antioxidant effect of FCOPs and its possible mechanism at the cellular level. Mouse embryonic fibroblasts NIH/3T3 and human vein endothelial cells (HUVECs) were exposed to 200 µM hydrogen peroxide containing different concentrations of FCOPs for 4 h and were supplemented with different concentrations of FCOPs for 24 h. Normal growth medium without FCOPs was applied for control cells. An array of assays was used to evaluate the implications of FCOPs on cellular oxidative stress status, cellular homeostasis, inflammatory levels, and mitochondrial function. We found that FCOPs exerted a protective effect by inhibiting reactive oxygen species (ROS) production, enhancing superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS) activities and cell viability, inhibiting cell cycle arrest in the G1 phase, suppressing interleukin-1β (IL-1β), IL-6, matrix metalloproteinase-3 (MMP-3) and intercellular adhesion molecule-1(ICAM-1) secretion, downregulating nuclear factor-kappa B (NF-κB) activity, protecting mitochondrial membrane potential, and increasing ATP synthesis and NAD+ activities in both cells. FCOPs had a stronger antioxidant impact on NIH/3T3 than on HUVECs, simultaneously increasing glutathione peroxidase (GSH-Px) activity and decreasing malondialdehyde (MDA) content in NIH/3T3. These findings indicate that FCOPs have antioxidant effects on different tissue cells damaged by oxidative stress. FCOPs were therefore found to promote cellular homeostasis, inhibit inflammation, and protect mitochondria. Meanwhile, better health outcomes will be achieved by thoroughly investigating the effective dose and intervention time of FCOPs, as the absorption efficiency of FCOPs varies in different tissue cells.