bluuebunny/crossref_metadata_2025 huggingface dataset:
PdMo nanoflowers for endogenous/exogenous-stimulated nanocatalytic therapy
Authors: Liang Xinqiang , Tang Yanping , Kurboniyon Mekhrdod S. , Luo Danni , Tu Guiwan , Xia Pengle , Ning Shufang , Zhang Litu , Wang Chen
Published: December 2023

The clinical application of reactive oxygen species (ROS)-mediated tumor treatment has been critically limited by inefficient ROS generation. Herein, we rationally synthesized and constructed the three-dimensional PdMo nanoflowers through a one-pot solvothermal reduction method for elaborately regulated peroxidase-like enzymatic activity and glutathione peroxidase-like enzymatic activity, to promote oxidation ROS evolvement and antioxidation glutathione depletion for achieving intensive ROS-mediated tumor therapy. The three-dimensional superstructure composed of two-dimensional nanosheet subunits can solve the issues by avoiding the appearance of tightly stacked crystalline nanostructures. Significantly, Mo is chosen as a second metal to alloy with Pd because of its more chemical valence and negative ionization energy than Pd for improved electron transfer efficiencies and enhanced enzyme-like activities. In addition, the photothermal effect generated by PdMo nanoflowers could also enhance its enzymatic activities. Thus, this work provides a promising paradigm for achieving highly ROS-mediated tumor therapeutic efficacy by regulating the multi-enzymatic activities of Pd-based nanoalloys.
The metabolism of nonstructural carbohydrates, lipids, and energy in two Cycas species with differential tolerance to unexpected freezing stress
Authors: Wu Yangyang , Zhu Huan , Ling Zhiwei , Lu Erya , Peng Xiaoling , Zheng Yanling
Published: December 2023

Introduction

With the climate warming, the occurrence of freezing events is projected to increase in late spring and early autumn in the Northern Hemisphere. Observation of morphological traits showed that Cycas panzhihuaensis was more tolerant to unexpected freezing stress than C. bifida. Energy balance is crucial for plant tolerance to stress. Here, we aimed to determine whether the different responses of the two species to the unpredicted freezing stress were associated with the metabolism of energy and related substances.

Methods

The effects of unexpected freezing temperatures on C. panzhihuaensis and C. bifida were studied by measuring chlorophyll fluorescence parameters, energy charge and the profile of nonstructural carbohydrates (NSC) and lipids.

Results

C. panzhihuaensis exhibited higher stability of photosynthetic machinery than C. bifida under unpredicted freezing events. Significant interaction between species and treatments were observed in the energy charge, the level of NSC and its most components and the amount of most lipid categories and lipid classes. The decrease of soluble sugar and the increase of neutral glycerolipids at the early freezing stage, the accumulation of membrane glycerolipids at the late freezing stage and the continuous decrease of energy charge during the freezing period were the characteristics of C. panzhihuaensis responding to unexpected freezing stress. The degradation of membrane glycerolipids and the continuous decrease of soluble sugar during the freezing period and the accumulation of neutral glycerolipids and energy charge at the late freezing stage represented the characteristics of C. bifida responses.

Discussion

The different freezing sensitivity between C. panzhihuaensis and C. bifida might be associated with the differential patterns of the metabolism of energy, NSC and lipids. C. panzhihuaensis possesses the potential to be introduced to the areas of higher latitudes and altitudes.
Responsivity and relation to depressive symptoms of occupational behavior and experience patterns
Authors: Papst Lilia , Zickwolf Christian , Käfer Michael , Beierlein Volker , Köllner Volker
Published: December 2023

Introduction

Work stress is a frequent factor in the development of depression. However, not only workplace environment, but also personal attitudes may affect stress experience. The aim of this study was to investigate the change sensitivity of occupational attitudes in psychosomatic inpatients and assess the relationship of changes to depressive symptom reduction.

Methods

The data set encompassed N = 1708 inpatients from two German psychosomatic rehabilitation clinics at admission and discharge. Responsivity of AVEM measures was evaluated by Bonferroni-corrected t-tests and Cohen’s dz effect sizes for paired samples. The relation of occupational behavior and experience patterns and depressive symptoms as assessed by the BDI-II questionnaire was calculated by Pearson correlation analysis of pre-post differential values.

Results

Changes in work attitudes were found on eight out of eleven AVEM subscales (Padj ≤ 0.001, Cohen’s dz = −0.45 to 0.43) and all AVEM coping styles. Most patients (57.4%) were classified to have a Burnout occupational coping style at admission. Changes following rehabilitation were most frequently observed from Burnout to Sparing coping styles (8.3%). Small to moderate associations between changes in occupational attitudes and depressive symptom reduction were found for all subscales (r = −0.39 to 0.25) except work ambition, and for occupational coping styles Burnout (r = 0.19), Sparing (r = −0.18) and Healthy (r = −0.10), but not Ambitious.

Discussion

The data support responsivity of occupational behavior and experience patterns within a psychosomatic rehabilitation setting. Correlations with depressive symptom reduction suggest that occupational attitudes are related but separate treatment targets.
Agronomic and reclamation strategies to enhance soil fertility, productivity and water accessibility
Authors: Rau Alexey , Zhu Kai , Nurlan Balgabayev , Mirobit Mirdadayev , Yessenkul Kalybekova , Bek Meymank Hozhaev , Nabiollina Madina , Kurmanbek Zhanymhan , Issakov Yerlan , Antal Sándor , Ujj Apolka , Dávid Lóránt Dénes
Published: December 2023

Introduction

Over the last decade, yields and profitability of crops on agricultural lands in the Kyzylkum irrigation massif in the south of Kazakhstan, as well as soil fertility, have been decreasing. The Kyzylkum irrigation basin, located in the Syrdarya River basin, is affected by various factors such as agronomic practices, reclamation factors, and human activities including tourism. This study aims to monitor the yields of cotton and alfalfa in alfalfa-cotton crop rotations in the Kyzylkum irrigation massif, assess soil fertility after alfalfa and cotton cultivation under production conditions, and evaluate the profitability of water consumption.

Methods

The research methodology involved experimental and observational studies conducted on a nine-pole alfalfa-cotton crop rotation area in the Kyzylkum basin within the Syrdarya river basin. The study was conducted on three farms: Didar, Toishy, and Ali-Myrza. Productivity, soil fertility after crop cultivation, and profitability of water consumption were determined through these studies.

Results

The study found that the Ali-Myrza farm experienced a 21% increase in cotton yield, a 24% increase in alfalfa yield, a 36% increase in profit from crop rotation, a 34% increase in profitability, and a 5-6% increase in water consumption. After alfalfa rotation, the Ali-Myrza farm recorded the highest cotton yield at 3.5 t/ha and a profit of 1,316 euros/ha. However, in the following year, cotton yields decreased to 2.52 t/ha, and profits dropped to 576 euro/ha. The first year of alfalfa cultivation resulted in a yield of 10 t/ha and a profit of 462 euro/ha, which increased to 14 t/ha and 967 euro/ha in the subsequent year. Soil analysis revealed that after two years of growing alfalfa in a six-pole rotation, the root mass in the top 20 cm of soil reached 8.89 t/ha. In contrast, rotations with seven (Toishy) and nine (Didar) crops resulted in lower root masses of 4.68 t/ha and 3.47 t/ha, respectively. The corresponding humus content was 1.65%, 1.40%, and 1.1%, respectively.

Discussion

Comparing the seven and nine-pole rotations of alfalfa and cotton, the six-pole rotation demonstrated significant improvements in soil fertility (30-40% increase), cotton yield (18-23% increase), alfalfa yield (20-28% increase), and water consumption (5-6% increase). These findings confirm the effectiveness of the alfalfa-cotton rotation method in enhancing soil fertility, productivity, water efficiency, and grey soil productivity in Kyzylkum irrigated fields. The results obtained from this study can be applied in cotton farms in South Kazakhstan that use nine-layer cotton and alfalfa rotations. Furthermore, the findings have implications for cotton cultivation in any country worldwide.
Enhanced Precision and Safety in Thermal Ablation: O-Arm Cone Beam Computed Tomography with Magnetic Resonance Imaging Fusion for Spinal Column Tumor Targeting
Authors: Aslan Siran , Al-Smadi Mohammad Walid , Kozma István , Viola Árpad
Published: December 2023

Spinal metastatic tumors are common and often cause debilitating symptoms. Image-guided percutaneous thermal ablation (IPTA) has gained significant recognition in managing spinal column tumors due to its exceptional precision and effectiveness. Conventional guidance modalities, including computed tomography, fluoroscopy, and ultrasound, have been important in targeting spinal column tumors while minimizing harm to adjacent critical structures. This study presents a novel approach utilizing a fusion of cone beam computed tomography with magnetic resonance imaging to guide percutaneous thermal ablation for four patients with secondary spinal column tumors. The visual analog scale (VAS) evaluated the procedure effectiveness during an 18-month follow-up. Percutaneous vertebroplasty was performed in two cases, and a thermostat was used during all procedures. Imaging was performed using the Stealth Station navigation system Spine 8 (SSS8) and a 1.5T MRI machine. The fusion of CBCT with MRI allowed for precise tumor localization and guidance for thermal ablation. Initial results indicate successful tumor ablation and symptom reduction, emphasizing the potential of CBCT–MRI fusion in spinal column tumor management. This innovative approach is promising in optimizing therapy for secondary spinal column tumors. Further studies are necessary to validate its efficacy and applicability.
Phytoplankton Diversity and Blooms in Ephemeral Saline Lakes of Cyprus
Authors: Polykarpou Polina , Katsiapi Matina , Genitsaris Savvas , Stefanidou Natassa , Dörflinger Gerald , Moustaka-Gouni Maria , Economou-Amilli Athena , Raitsos Dionysios E.
Published: December 2023

The ephemeral saline lakes of Cyprus in the Mediterranean, situated in close proximity to each other, demonstrate pronounced seasonal and interannual fluctuations in their environmental conditions. Despite their extreme saline conditions, these lakes support phytoplankton diversity and bloom-forming species. Anthropogenic activities, particularly urban and artificial land uses within their catchments, contribute to eutrophication, warranting conservation attention within the context of European legislation. Over two years (2018–2019), we examined phytoplankton abundance and diversity alongside salinity in six lakes, with samples collected every three weeks. Chlorophytes were the dominant and most diverse group, followed by cyanobacteria and diatoms. Increasing salinity correlated with reduced compositional diversity and species richness. The proximity of lakes to each other suggested airborne microbe colonization from one lake to another as a significant factor in shaping these communities, while similar land use within each lake’s catchment impacted bloom formation. The highly halotolerant chlorophyte Dunaliella frequently dominated phytoplankton blooms, occasionally coexisting with other taxa in less saline lakes. Our findings provide insight into the phytoplankton community dynamics in temporal saline lakes, essential for developing effective conservation strategies and sustainable management practices.
Optimal Configuration Analysis Method of Energy Storage System Based on “Equal Area Criterion”
Authors: Li Yizheng , Zeng Yuan , Wang Zhidong , Zhao Lang , Wang Yao
Published: December 2023

In order to solve the problem of randomness and volatility caused by the rapid growth of renewable energy (RE), energy storage systems (ESSs)—as an important means of regulation—can effectively improve the flexible regulation capacity of power systems utilizing a high proportion of RE. Most of the current ESS capacity configuration procedures are carried out based on the typical scenario method or time series production simulation. This method tends to determine the size of the ESS configuration through multiple trial simulations. Uncertainty of simulation prediction data can result in the existence of an excess capacity or lack of configured capacity. In addition, this method reflects the ESS demand under specific targets, but it fails to fully utilize RE generation characteristics. The configuration process lacks the mathematical mechanism of RE consumption, and the calculation process is too complicated. In view of the shortcomings of traditional ESS optimal configuration methods, this paper examines the mathematical mechanism of RE consumption and proposes the ESS optimal configuration analysis method based on “equal area criterion”. First, the principle of RE consumption is analyzed and the “RE consumption characteristic curve” is proposed according to RE characteristics. In addition, a working principle diagram of RE consumption, including ESS, is constructed to visually show the consumption capacity of RE and the working position of ESS. Then, the ESS optimal configuration process, based on the “equal area criterion”, is proposed to achieve an accurate match between ESS capacity demand and RE consumption targets. Finally, the power grid of a region in China is taken as an example. We prove that the proposed method can save 1.41 × 103 MWh of ESS capacity and provide a more “mathematical” and “convenient” systematic solution for RE consumption and ESS optimization compared to the production simulation method.
Prediction of Circumsolar Irradiance and Its Impact on CSP Systems under Clear Skies
Authors: Abreu Edgar F.M. , Canhoto Paulo , Costa Maria João
Published: December 2023

In this work, a model to estimate circumsolar normal irradiance (CSNI) for several half-opening angles under clear skies was developed. This approach used a look-up table to determine the model parameters and estimate CSNI for half-opening angles between 0.5° and 5°. To develop and validate the proposed model, data from five locations worldwide were used. It was found that the proposed model performs better at the locations under study than the models available in the literature, with relative mean bias error ranging from −13.94% to 0.70%. The impact of CSNI for these different half-opening angles on concentrating solar power (CSP) systems was also studied. It was found that neglecting CSNI could lead to up to a 7% difference between the direct normal irradiance (DNI) measured by a field pyrheliometer and the DNI that is captured by CSP systems. Additionally, a case study for parabolic trough concentrators was performed as a way to estimate the impact of higher circumsolar ratios (CSR) on the decrease of the intercept factor for these systems. It was also concluded that if parabolic trough designers aim to reduce the impact of CSNI variation on the intercept factor, then parabolic troughs with higher rim angles are preferred.
A Review on the Effect of Electrical Steel Manufacturing Processes on the Performance of Electric Machines
Authors: Mahmouditabar Farshid , Baker Nick
Published: December 2023

The manufacturing processing of Electrical Steel Laminations (ESLs) for electric machines comprises cutting, stacking, and housing techniques which can result in plastic deformation and residual stress in the soft magnetic material. These manufacturing processes result in decreasing the magnetic quality and a local increase in both the static and dynamic hysteresis losses near the cut edges and consequently a reduction in the performance of the designed motor. The iron losses resulting from cutting can vary by a factor of two or more depending on geometrical, material, and processing parameters as well as the magnetic field strength. It is advantageous to consider these manufacturing effects in the design stage to reduce the probability of underperforming mass production. In this paper, the manufacturing processes of ESLs and their modelling methods are comprehensively surveyed. The gaps in scientific understanding and the research need for the expansion of accurate modelling of the cutting and joining of ESLs are subsequently discussed.
Research on Estimating and Evaluating Subtropical Forest Carbon Stocks by Combining Multi-Payload High-Resolution Satellite Data
Authors: Du Yisha , Chen Donghua , Li Hu , Liu Congfang , Liu Saisai , Zhang Naiming , Fan Jingwei , Jiang Deting
Published: December 2023

Forest carbon stock is an important indicator reflecting the structure of forest ecosystems and forest quality, and an important parameter for evaluating the carbon sequestration capacity and carbon balance of forests. It is of great significance to study forest carbon stock in the context of current global climate change. To explore the application ability of multi-loaded, high-resolution satellite data in the estimation of subtropical forest carbon stock, this paper takes Huangfu Mountain National Forest Park in Chuzhou City as the study area, extracts remote sensing features such as spectral features, texture features, backscattering coefficient, and other remote sensing features based on multi-loaded, high-resolution satellite data, and carries out correlation analyses with the carbon stock of different species of trees and different age groups of forests. Regression models for different tree species were established for different data sources, and the optimal modeling factors for multi-species were determined. Then, three algorithms, namely, multiple stepwise regression, random forest, and gradient-enhanced decision tree, were used to estimate carbon stocks of multi-species, and the predictive ability of different estimation models on carbon stocks was analyzed using the coefficient of determination (R2) and the root mean square error (RMSE) as indexes. The following conclusions were drawn: for the feature factors, the texture features of the GF-2 image, the new red edge index of the GF-6 image, the radar intensity coefficient sigma, and radar brightness coefficient beta of the GF-3 image have the best correlation with the carbon stock; for the algorithms, the random forest and gradient-boosting decision tree have the better effect of fitting and predicting the carbon stock of multi-tree species, among which gradient-boosting decision tree has the best effect, with an R2 of 0.902 and an RMSE of 10.261 t/ha. In summary, the combination of GF-2, GF-3, and GF-6 satellite data and gradient-boosting decision tree obtains the most accurate estimation results when estimating forest carbon stocks of complex tree species; multi-load, high-resolution satellite data can be used in the inversion of subtropical forest parameters to estimate the carbon stocks of subtropical forests. The multi-loaded, high-resolution satellite data have great potential for application in the field of subtropical forest parameter inversion.
Genome-Wide Association Study of Arsenic Accumulation in Polished Rice
Authors: Dong Zheng , Guo Liang , Li Xiaoxiang , Li Yongchao , Liu Wenqiang , Chen Zuwu , Liu Licheng , Liu Zhixi , Guo Yujing , Pan Xiaowu
Published: December 2023

The accumulation of arsenic (As) in rice poses a significant threat to food safety and human health. Breeding rice varieties with low As accumulation is an effective strategy for mitigating the health risks associated with arsenic-contaminated rice. However, the genetic mechanisms underlying As accumulation in rice grains remain incompletely understood. We evaluated the As accumulation capacity of 313 diverse rice accessions grown in As-contaminated soils with varying As concentrations. Six rice lines with low As accumulation were identified. Additionally, a genome-wide association studies (GWAS) analysis identified 5 QTLs significantly associated with As accumulation, with qAs4 being detected in both of the experimental years. Expression analysis demonstrated that the expression of LOC_Os04g50680, which encodes an MYB transcription factor, was up-regulated in the low-As-accumulation accessions compared to the high-As-accumulation accessions after As treatment. Therefore, LOC_Os04g50680 was selected as a candidate gene for qAs4. These findings provide insights for exploiting new functional genes associated with As accumulation and facilitating the development of low-As-accumulation rice varieties through marker-assisted breeding.
Cybersex and Attachment Styles: Proposal of the Emotional and Relational Aspects in Cybersex Activities (ERACA) Questionnaire
Authors: Baroncelli Andrea , Taddei Maria Giulia , Giommi Roberta , Lenzi Elena , Facci Carolina , Ciucci Enrica
Published: December 2023

The current study presents the development and the initial validation of a new questionnaire to assess individual differences in emotional and relational aspects related to cybersex activities (i.e., the ERACA). A total of 246 adults (105 females, mean age = 31.89 years, SD = 10.03) coming from the general adult population participated in the study. The items of the ERACA were developed considering the extant literature, and an exploratory factor analysis approach indicated a three-factor structure (i.e., the gratification of the Self through the objectification of other people, the gratification of the Self through relational aspects, betrayal, and infidelity). The associations between the dimensions of the ERACA and dimensional measures of both attachment styles and online sexual behaviors indicated that different aspects related to the quality of the relationships play a different role in individual differences concerning emotional and relational aspects of cybersex activities. The discussion emphasizes the potential usefulness of the ERACA questionnaire for both research purposes and from a health-promoting point of view.
Targeting Cathepsin L in Cancer Management: Leveraging Machine Learning, Structure-Based Virtual Screening, and Molecular Dynamics Studies
Authors: Almalki Abdulraheem Ali , Shafie Alaa , Hazazi Ali , Banjer Hamsa Jameel , Bakhuraysah Maha M. , Almaghrabi Sarah Abdullah , Alsaiari Ahad Amer , Alsaeedi Fouzeyyah Ali , Ashour Amal Adnan , Alharthi Afaf , Alharthi Nahed S. , Anjum Farah
Published: December 2023

Cathepsin L (CTSL) expression is dysregulated in a variety of cancers. Extensive empirical evidence indicates their direct participation in cancer growth, angiogenic processes, metastatic dissemination, and the development of treatment resistance. Currently, no natural CTSL inhibitors are approved for clinical use. Consequently, the development of novel CTSL inhibition strategies is an urgent necessity. In this study, a combined machine learning (ML) and structure-based virtual screening strategy was employed to identify potential natural CTSL inhibitors. The random forest ML model was trained on IC50 values. The accuracy of the trained model was over 90%. Furthermore, we used this ML model to screen the Biopurify and Targetmol natural compound libraries, yielding 149 hits with prediction scores >0.6. These hits were subsequently selected for virtual screening using a structure-based approach, yielding 13 hits with higher binding affinity compared to the positive control (AZ12878478). Two of these hits, ZINC4097985 and ZINC4098355, have been shown to strongly bind CTSL proteins. In addition to drug-like properties, both compounds demonstrated high affinity, ligand efficiency, and specificity for the CTSL binding pocket. Furthermore, in molecular dynamics simulations spanning 200 ns, these compounds formed stable protein-ligand complexes. ZINC4097985 and ZINC4098355 can be considered promising candidates for CTSL inhibition after experimental validation, with the potential to provide therapeutic benefits in cancer management.
Oxidative Stress and the Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) Pathway in Multiple Sclerosis: Focus on Certain Exogenous and Endogenous Nrf2 Activators and Therapeutic Plasma Exchange Modulation
Authors: Tonev Dimitar , Momchilova Albena
Published: December 2023

The pathogenesis of multiple sclerosis (MS) suggests that, in genetically susceptible subjects, T lymphocytes undergo activation in the peripheral compartment, pass through the BBB, and cause damage in the CNS. They produce pro-inflammatory cytokines; induce cytotoxic activities in microglia and astrocytes with the accumulation of reactive oxygen species, reactive nitrogen species, and other highly reactive radicals; activate B cells and macrophages and stimulate the complement system. Inflammation and neurodegeneration are involved from the very beginning of the disease. They can both be affected by oxidative stress (OS) with different emphases depending on the time course of MS. Thus, OS initiates and supports inflammatory processes in the active phase, while in the chronic phase it supports neurodegenerative processes. A still unresolved issue in overcoming OS-induced lesions in MS is the insufficient endogenous activation of the Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) pathway, which under normal conditions plays an essential role in mitochondria protection, OS, neuroinflammation, and degeneration. Thus, the search for approaches aiming to elevate endogenous Nrf2 activation is capable of protecting the brain against oxidative damage. However, exogenous Nrf2 activators themselves are not without drawbacks, necessitating the search for new non-pharmacological therapeutic approaches to modulate OS. The purpose of the present review is to provide some relevant preclinical and clinical examples, focusing on certain exogenous and endogenous Nrf2 activators and the modulation of therapeutic plasma exchange (TPE). The increased plasma levels of nerve growth factor (NGF) in response to TPE treatment of MS patients suggest their antioxidant potential for endogenous Nrf2 enhancement via NGF/TrkA/PI3K/Akt and NGF/p75NTR/ceramide-PKCζ/CK2 signaling pathways.
Identification of New GSK3β Inhibitors through a Consensus Machine Learning-Based Virtual Screening
Authors: Galati Salvatore , Di Stefano Miriana , Bertini Simone , Granchi Carlotta , Giordano Antonio , Gado Francesca , Macchia Marco , Tuccinardi Tiziano , Poli Giulio
Published: December 2023

Glycogen synthase kinase-3 beta (GSK3β) is a serine/threonine kinase that plays key roles in glycogen metabolism, Wnt/β-catenin signaling cascade, synaptic modulation, and multiple autophagy-related signaling pathways. GSK3β is an attractive target for drug discovery since its aberrant activity is involved in the development of neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. In the present study, multiple machine learning models aimed at identifying novel GSK3β inhibitors were developed and evaluated for their predictive reliability. The most powerful models were combined in a consensus approach, which was used to screen about 2 million commercial compounds. Our consensus machine learning-based virtual screening led to the identification of compounds G1 and G4, which showed inhibitory activity against GSK3β in the low-micromolar and sub-micromolar range, respectively. These results demonstrated the reliability of our virtual screening approach. Moreover, docking and molecular dynamics simulation studies were employed for predicting reliable binding modes for G1 and G4, which represent two valuable starting points for future hit-to-lead and lead optimization studies.
Predicting the Impact of OTOF Gene Missense Variants on Auditory Neuropathy Spectrum Disorder
Authors: Dmitriev Dmitry A. , Shilov Boris V. , Polunin Michail M. , Zadorozhny Anton D. , Lagunin Alexey A.
Published: December 2023

Auditory neuropathy spectrum disorder (ANSD) associated with mutations of the OTOF gene is one of the common types of sensorineural hearing loss of a hereditary nature. Due to its high genetic heterogeneity, ANSD is considered one of the most difficult hearing disorders to diagnose. The dataset from 270 known annotated single amino acid substitutions (SAV) related to ANSD was created. It was used to estimate the accuracy of pathogenicity prediction using the known (from dbNSFP4.4) method and a new one. The new method (ConStruct) for the creation of the protein-centric classification model is based on the use of Random Forest for the analysis of missense variants in exons of the OTOF gene. A system of predictor variables was developed based on the modern understanding of the structure and function of the otoferlin protein and reflecting the location of changes in the tertiary structure of the protein due to mutations in the OTOF gene. The conservation values of nucleotide substitutions in genomes of 100 vertebrates and 30 primates were also used as variables. The average prediction of balanced accuracy and the AUC value calculated by the 5-fold cross-validation procedure were 0.866 and 0.903, respectively. The model shows good results for interpreting data from the targeted sequencing of the OTOF gene and can be implemented as an auxiliary tool for the diagnosis of ANSD in the early stages of ontogenesis. The created model, together with the results of the pathogenicity prediction of SAVs via other known accurate methods, were used for the evaluation of a manually created set of 1302 VUS related to ANSD. Based on the analysis of predicted results, 16 SAVs were selected as the new most probable pathogenic variants.
Rate of Leiomyosarcomas during Surgery for Uterine Fibroids: 8-Year Experience of a Single Center
Authors: Zouzoulas Dimitrios , Tsolakidis Dimitrios , Pavlidi Olga I. , Pappas Panagiotis D. , Theodoridis Theodoros , Pados George , Pervana Stavroula , Pazarli Elsa , Grimbizis Grigoris
Published: December 2023

The aim of this study is to investigate the prevalence of occult malignant mesenchymal tumors in patients operated on for uterine fibroids in relation to the surgical approach and type of operation. A retrospective review of all patients that underwent surgery for uterine fibroids (January 2011–December 2018) at the 1st Department of Obstetrics & Gynecology at “Papageorgiou” Hospital. The surgical approach and clinicopathological characteristics were analyzed. A total of 803 patients were operated on: 603 (75.1%) with laparotomy, 187 (23.3%) laparoscopically, and 13 (1.6%) vaginally. Furthermore, 423 (52.7%) patients underwent hysterectomy and 380 (47.3%) myomectomies. Laparoscopy and myomectomy were offered to younger patients with fewer smaller uterine fibroids and were associated with statistically significant shorter hospitalization. The pathological reports revealed: 690 (86%) benign leiomyomas, 32 (4%) cellular leiomyomas, 29 (3.6%) degenerated leiomyomas, 22 (2.7%) adenomyomas, 18 (2.2%) atypical-bizarre leiomyomas, 1 (0.1%) STUMP, 5 (0.65%) endometrial stromal sarcomas, and 6 (0.75%) cases of leiomyosarcomas (LMS). All LMS were preoperatively characterized as suspicious and underwent abdominal hysterectomy. Morcellation was offered in two cases of atypical leiomyomas, with no morcellation-associated complication. Laparoscopy as a valuable surgical approach for young patients with fewer in number and smaller in size fibroids is associated with shorter hospitalization. The risk of unintended morcellation of LMS seems to be very low and can be reduced with careful preoperative work-up but not eliminated.
Eco-Friendly Pavements Manufactured from Mixed Recycled Aggregates Obtained from Construction and Demolition Waste: An Industrial-Scale Validation
Authors: Contreras-Llanes Manuel , Gázquez Manuel Jesús , Romero Maximina
Published: December 2023

This study aimed to validate that laboratory-scale results could be commercially replicated when manufacturing marketable precast concrete. Construction and demolition waste (CDW) was separated into two (fine and coarse) recycled aggregates (RAs). Precast paver and kerb units were fabricated by partial or total substitution of natural aggregates (NAs) by RAs. The study involved the comprehensive characterisation of raw materials, including particle size distribution, mineral composition, and elemental composition. Paver blocks and kerbs manufactured with up to 50% RAs showed mechanical resistance (T = 3.7 ± 0.2 and B = 5.3 ± 0.6 MPa, respectively), water absorption between 5.3–5.7%, and abrasion resistance (approximately 20.2 mm), which met the standard requirements (UNE-EN 1340:2004 and UNE-EN 1338:2004). Furthermore, industrial-scale precast pavement units demonstrated strength and durability suitable for heavy traffic areas. A reduction of 13% in cement content could maintain the requirements with a partial RA substitution of 25%, offering economic and environmental benefits. Therefore, it is feasible at an industrial level to replace NAs with RAs, promoting durability and technological properties with a positive environmental impact and considerably reducing CO2 emissions by up to 65%. Overall, pavers with RAs manufactured at the laboratory scale met mechanical standards, and the kerb stones showed improvements in abrasion resistance. On an industrial scale, kerb stones and precast blocks with specific substitutions can meet strength, water absorption, and abrasion requirements, allowing a reduction in cement content.
Cytosporones with Anti-Inflammatory Activities from the Mangrove Endophytic Fungus Phomopsis sp. QYM-13
Authors: Wang Guisheng , Yuan Yilin , Li Zhaokun , Zhu Junhao , She Zhigang , Chen Yan
Published: December 2023

Six previously undescribed cytosporone derivatives (phomotones A-E (1–5) and phomotone F (13)), two new spiro-alkanol phombistenes A-B (14–15), and seven known analogs (6–12) were isolated from the mangrove endophytic fungus Phomopsis sp. QYM-13. The structures of these compounds were elucidated using spectroscopic data analysis, electronic circular dichroism (ECD), and 13C NMR calculations. Compound 14 features an unprecedented 1,6-dioxaspiro[4.5]decane ring system. All isolates were evaluated for their inhibitory effect on nitric oxide (NO) in LPS-induced RAW264.7 cells. The results showed that compounds 1, 6, 8, and 11 exhibited potent bioactivities by comparing with positive control. Then, compound 1 displayed the anti-inflammatory effect by inhibiting the MAPK/NF-κB signaling pathways. Molecular docking further revealed the possible mechanism of compound 1 interaction with ERK protein.
Size- and Voltage-Dependent Electron Transport of C2N-Rings-Based Molecular Chains
Authors: Song Dian , Li Jie , Liu Kun , Guo Junnan , Li Hui , Okulov Artem
Published: December 2023

C2N-ring-based molecular chains were designed at the molecular level and theoretically demonstrated to show distinctive and valuable electron transport properties that were superior to the parent carbonaceous system and other similar nanoribbon-based molecular chains. This new -type molecular chain presented an exponential attenuation of the conductance and electron transmission with the length. Essentially, the molecular chain retained the electron-resonant tunneling within 7 nm and the dominant transport orbital was the LUMO. Shorter molecular chains with stronger conductance anomalously possessed a larger tunnel barrier energy, attributing to the compensation of a much smaller HOMO–LUMO gap, and these two internal factors codetermined the transport capacity. Some influencing factors were also studied. In contrast to the common O impurity with a tiny effect on electron transmission of the C2N rings chain, the common H impurity clearly improved it. When the temperature was less than 400 K, the electron transmission varied with temperature within a narrow range, and the structural disorder deriving from proper heating did not greatly modify the transmission possibility and the exponentially decreasing tendency with the length. In a non-equilibrium condition, the current increased overall with the bias but the growth rate varied with size. A valuable negative differential resistance (NDR) effect appeared in longer molecular chains with an even number of big carbon–nitrogen rings and strengthened with size. The emergence of such an effect originated from the reduction in transmission peaks. The conductance of longer molecular chains was enhanced with the voltage but the two shortest ones presented completely different trends. Applying the bias was demonstrated to be an effective way for C2N-ring-based molecular chains to slow down the conductance decay constant and affect the transport regime. C2N-ring-based molecular chains show a perfect application in tunneling diodes and controllable molecular devices.
From Selye’s and Szabo’s Cysteamine-Duodenal Ulcer in Rats to Dopamine in the Stomach: Therapy Significance and Possibilities
Authors: Sikiric Predrag , Boban Blagaic Alenka , Krezic Ivan , Zizek Helena , Kalogjera Luka , Smoday Ivan Maria , Vukovic Vlasta , Oroz Katarina , Chiddenton Helen Marie , Buric Sara , Antunovic Marko , Gojkovic Slaven , Strbe Sanja , Skocic Milena , Sikiric Suncana , Milavic Marija , Beketic Oreskovic Lidija , Kokot Antonio , Koprivanac Antun , Dobric Ivan , Sever Marko , Staresinic Mario , Batelja Vuletic Lovorka , Skrtic Anita , Seiwerth Sven
Published: December 2023

We reviewed gastric ulcer healing by dopamine considering several distinctive duodenal key points. Selye and Szabo describe the cysteamine-induced duodenal ulcer in rats as a duodenal stress ulcer in patients. Szabo’s cysteamine duodenal ulcer as the dopamine duodenal healing and cysteamine as a dopamine antagonist signifies the dopamine agonists anti-ulcer effect and dopamine antagonists ulcerogenic effect. From these viewpoints, we focused on dopamine and gastric ulcer healing. We mentioned antecedent studies on the dopamine presence in the stomach and gastric juice. Then we reviewed, in the timeline, therapy significance arising from the anti-ulcer potency of the various dopamine agonists, which is highly prevailing over the quite persistent beneficial evidence arising from the various dopamine antagonists. Meanwhile, the beneficial effects of several peptides (i.e., amylin, cholecystokinin, leptin, and stable gastric pentadecapeptide BPC 157, suggested as an acting mediator of the dopamine brain-gut axis) were included in the dopamine gastric ulcer story. We attempt to resolve dopamine agonists/antagonists issue with the dopamine significance in the stress (cysteamine as a prototype of the duodenal stress ulcer), and cytoprotection (cysteamine in small dose as a prototype of the cytoprotective agents; cysteamine duodenal ulcer in gastrectomized rats). Thereby, along with dopamine agonists’ beneficial effects, in special circumstances, dopamine antagonists having their own ulcerogenic effect may act as “mild stress (or)” or “small irritant” counteracting subsequent strong alcohol or stress procedure-induced severe lesions in this particular tissue. Finally, in the conclusion, as a new improvement in further therapy, we emphasized the advantages of the dopamine agents’ application in lower gastrointestinal tract therapy.
Carrier–Tumor Cell Membrane Interactions for Optimized Delivery of a Promising Drug, 4(RS)-4-F4t-Neuroprostane
Authors: Abawi Ariana , Thomann Céline , Lollo Giovanna , Granjon Thierry , Petiot Emma , Bérot Anna , Oger Camille , Bultel-Poncé Valérie , Guy Alexandre , Galano Jean-Marie , Durand Thierry , Girard-Egrot Agnès , Maniti Ofelia
Published: December 2023

Nanomedicines engineered to deliver molecules with therapeutic potentials, overcoming drawbacks such as poor solubility, toxicity or a short half-life, are targeted towards their cellular destination either passively or through various elements of cell membranes. The differences in the physicochemical properties of the cell membrane between tumor and nontumor cells have been reported, but they are not systematically used for drug delivery purposes. Thus, in this study, a new approach based on a match between the liposome compositions, i.e., membrane fluidity, to selectively interact with the targeted cell membrane was used. Lipid-based carriers of two different fluidities were designed and used to deliver 4(RS)-4-F4t-Neuroprostane (F4t-NeuroP), a potential antitumor molecule derived from docosahexaenoic acid (DHA). Based on its hydrophobic character, F4t-NeuroP was added to the lipid mixture prior to liposome formation, a protocol that yielded over 80% encapsulation efficiency in both rigid and fluid liposomes. The presence of the active molecule did not modify the liposome size but increased the liposome negative charge and the liposome membrane fluidity, which suggested that the active molecule was accommodated in the lipid membrane. F4t-NeuroP integration in liposomes with a fluid character allowed for the selective targeting of the metastatic prostate cell line PC-3 vs. fibroblast controls. A significant decrease in viability (40%) was observed for the PC-3 cancer line in the presence of F4t-NeuroP fluid liposomes, whereas rigid F4t-NeuroP liposomes did not alter the PC-3 cell viability. These findings demonstrate that liposomes encapsulating F4t-NeuroP or other related molecules may be an interesting model of drug carriers based on membrane fluidity.
Development and Characterization of Thermoresponsive Smart Self-Adaptive Chitosan-Based Polymer for Wellbore Plugging
Authors: Wu Huimei , Lou Yishan , Li Zhonghui , Zhai Xiaopeng , Gao Fei
Published: December 2023

To meet the escalating demand for oil and gas exploration in microporous reservoirs, it has become increasingly crucial to develop high-performance plugging materials. Through free radical grafting polymerization technology, a carboxymethyl chitosan grafted poly (oligoethylene glycol) methyl ether methyl methacrylate acrylic acid copolymer (CCMMA) was successfully synthesized. The resulting CCMMA exhibited thermoresponsive self-assembling behavior. When the temperature was above its lower critical solution temperature (LCST), the nanomicelles began to aggregate, forming mesoporous aggregated structures. Additionally, the electrostatic repulsion of AA chains increased the value of LCST. By precisely adjusting the content of AA, the LCST of CCMMA could be raised from 84.7 to 122.9 °C. The rheology and filtration experiments revealed that when the temperature surpassed the switching point, CCMMA exhibited a noteworthy plugging effect on low-permeability cores. Furthermore, it could be partially released as the temperature decreased, exhibiting temperature-switchable and self-adaptive plugging properties. Meanwhile, CCMMA aggregates retained their reversibility, along with thermal thickening behavior in the pores. However, more detailed experiments and analysis are needed to validate these claims, such as a comprehensive study of the CCMMA copolymer’s physical properties, its interaction with the reservoir environment, and its performance under various conditions. Additionally, further studies are required to optimize its synthesis process and improve its efficiency as a plugging material for oil and gas recovery in microporous reservoirs.
Polyhydroxyurethane and Poly(ethylene oxide) Multiblock Copolymer Networks: Crosslinking with Polysilsesquioxane, Reprocessing and Solid Polyelectrolyte Properties
Authors: Li Lei , Zhao Bingjie , Hang Guohua , Gao Yuan , Hu Jiawei , Zhang Tao , Zheng Sixun
Published: December 2023

This contribution reports the synthesis of polyhydroxyurethane (PHU)-poly(ethylene oxide) (PEO) multiblock copolymer networks crosslinked with polysilsesquioxane (PSSQ). First, the linear PHU-PEO multiblock copolymers were synthesized via the step-growth polymerization of bis(6-membered cyclic carbonate) (B6CC) with α,ω-diamino-terminated PEOs with variable molecular weights. Thereafter, the PHU-PEO copolymers were allowed to react with 3-isocyanatopropyltriethoxysilane (IPTS) to afford the derivatives bearing triethoxysilane moieties, the hydrolysis and condensation of which afforded the PHU-PEO networks crosslinked with PSSQ. It was found that the PHU-PEO networks displayed excellent reprocessing properties in the presence of trifluoromethanesulfonate [Zn(OTf)2]. Compared to the PHU networks crosslinked via the reaction of difunctional cyclic carbonate with multifunctional amines, the organic–inorganic PHU networks displayed the decreased reprocessing temperature. The metathesis of silyl ether bonds is responsible for the improved reprocessing behavior. By adding lithium trifluoromethanesulfonate (LiOTf), the PHU-PEO networks were further transformed into the solid polymer electrolytes. It was found that the crystallization of PEO chains in the crosslinked networks was significantly suppressed. The solid polymer electrolytes had the ionic conductivity as high as 7.64 × 10−5 S × cm−1 at 300 K. More importantly, the solid polymer electrolytes were recyclable; the reprocessing did not affect the ionic conductivity.
Construction and Characterization of Fitting Equations for a New Wheat Straw Pulping Method
Authors: Liang Xiaoli , Wei Shan , Xu Yanpeng , Yin Liang , Wang Ruiming , Li Piwu , Liu Kaiquan
Published: December 2023

The pretreatment of pulp with enzymes has been extensively studied in the laboratory. However, due to cost constraints, the application of enzymes in the pulp and paper industry is very limited. In this paper, an environment-friendly and efficient pulping method is proposed as an alternative to traditional pulping and papermaking methods. This new method overcomes the low efficiency and extreme pollution problems associated with traditional pulping methods. In addition, fitting equations for the new pulping method are constructed using data on enzyme treatments, which reflect the effect of enzymes and enable the realization of real-time control of the pulping process. The experimental results show that the efficiency of the pulping and papermaking process can be improved using biological enzymes, and the separation of cellulose can be facilitated using mixed enzymes, which have a better effect than single enzymes.
Analysis of Light Obstruction from Street Lighting in Road Scenes
Authors: Ren Jingzhi , Li Yongqiang , Liu Huiyun , Li Kanghong , Hao Daoqian , Wang Zhiyao
Published: December 2023

As urban greenery improves and the ecological environment is continuously optimized, road facilities are also impacted to varying degrees. For example, as vegetation grows, it causes varying degrees of obstruction to the lighting facilities on the roads. This article is based on vehicle-mounted LiDAR data and focuses on the point cloud data characteristics of different objects. Using appropriate modeling techniques, it accurately models road surfaces, green belts, streetlights, and other objects. On the Lumion platform, this system creates a 3D visualization of road scenes and examines the interplay between objects and lighting space, analyzing lit areas. Leveraging the precise 3D spatial relationships found in point clouds, it determines the effective illumination area on the ground from streetlights after object obstruction, comparing it to the theoretical illumination area. This not only visualizes the road scene but also quantifies the lighting obstruction rate. Furthermore, it assesses the lighting conditions in road scenes based on illuminance distribution, offering scientific insights and suggestions for enhancing road lighting.