bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Analysis of Fecal Microbiome and Metabolomics in Hepatic Fibrosis Rats Reveal Hepatoprotective Effects of Yinchen Wuling Powder From the Host-Microbial Metabolic Axis
Authors: Zhang Yumeng , Zhao Min , Jiang Xue , Qiao Qiaoyu , Liu Tingting , Zhao Chunjie , Wang Miao
Published: July 2021

Hepatic fibrosis (HF) is a typical consequence in the development of multiple chronic liver diseases, which is intimately related to the composition and metabolic status of gut microbiota. A myriad of evidence has indicated that traditional Chinese medicine can treat HF by regulating gut microbiota. Yinchen Wuling powder (YCWLP) is a famous traditional Chinese medicine prescription, which has been used to relieve liver diseases for thousands of years. YCWLP has demonstrated protective function on HF, but its effect on the alterations of gut microbiota is still unclear, and its explicit therapeutic mechanism also needs to be further elucidated. In this study, 16S rRNA gene sequencing and fecal metabolomics analysis were combined to investigate the influence of YCWLP on gut microbiota in HF rats and the interactions between gut microbiota and host metabolism. The results showed that YCWLP treatment significantly improved the disorder of multiple organ indices, HF-related cytokines and plasma LPS induced by HF. Masson’s trichrome stainings also showed that YCWLP treatment could significantly alleviate the severity of HF in rats. Additionally, YCWLP could reverse the significant changes in the abundance of certain genera closely related to HF phenotype, including Barnesiella [Ruminococcus] and Christensenella. Meanwhile, YCWLP significantly increased the abundance of Bifidobacterium, Coprococcus and Anaerostipes, which are closely related to butyrate production. Metabolomics and Spearman’s correlation analysis showed that YCWLP could regulate the disorder of arginine biosynthesis, sphingolipid metabolism and alanine, aspartate and glutamate metabolism in HF rats, and these regulations were intimately related to Barnesiella, [Ruminococcus], Christensenella, Coprococcus and Anaerostipes. By explaining the biological significance of the above results, we concluded that YCWLP might ameliorate HF by regulating the imbalance of gut microbiota, increasing the abundance of butyrate-producing bacteria to reduce ammonia production, promote ammonia degradation, and regulate pro-inflammatory cytokines and immune function.
MALAT1: A Pivotal lncRNA in the Phenotypic Switch of Gastric Smooth Muscle Cells via the Targeting of the miR-449a/DLL1 Axis in Diabetic Gastroparesis
Authors: Wang Yanjuan , Wang Yan , Zhu Boqian , Zhu Ying , Jiang Ya , Xiong Wenjie , Lin Lin , Gong Yaoyao
Published: July 2021

Diabetic gastroparesis (DGP) is a common complication of diabetes mellitus (DM). Our previous study suggested that the expression of the long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is closely related to DGP. However, the role of MALAT1 in DGP pathogenesis remains unclear. Here, we aim to characterize the role of MALAT1 in DGP. First, we analyzed the lncRNA expression profiles through lncRNA sequencing. Next, we detected MALAT1 expression in the stomach tissues of DGP model mice and diabetic patients. Then, we investigated the role and mechanisms of MALAT1 in the proliferation, migration, phenotypic switch, and carbachol-induced intracellular Ca2+ changes in human gastric smooth muscle cells (HGSMCs) under high glucose (HG) conditions, using short hairpin RNA technology, RNA immunoprecipitation, and dual-luciferase reporter assays. We show that MALAT1 expression was upregulated in the gastric tissues of DGP model mice, the adjacent healthy tissues collected from diabetic gastric cancer patients with DGP symptoms, and in HGSMCs cultured under HG conditions. Functionally, MALAT1 knockdown in vitro impacted the viability, proliferation, migration and promoted the phenotypic switch of HGSMCs under HG conditions. Additionally, we show that MALAT1 sponged miR-449a, regulating Delta-like ligand 1 (DLL1) expression in HGSMCs; any disturbance of the MALAT1/miR-449a/DLL1 pathway affects the proliferation, migration, phenotypic switch, and carbachol-induced Ca2+ transient signals in HGSMCs under HG conditions. Collectively, our data highlight a novel regulatory signaling pathway, the MALAT1/miR-449a/DLL1 axis, in the context of DGP.
DIlp7-Producing Neurons Regulate Insulin-Producing Cells in Drosophila
Authors: Prince Elodie , Kretzschmar Jenny , Trautenberg Laura C. , Broschk Susanne , Brankatschk Marko
Published: July 2021

Cellular Insulin signaling shows a remarkable high molecular and functional conservation. Insulin-producing cells respond directly to nutritional cues in circulation and receive modulatory input from connected neuronal networks. Neuronal control integrates a wide range of variables including dietary change or environmental temperature. Although it is shown that neuronal input is sufficient to regulate Insulin-producing cells, the physiological relevance of this network remains elusive. In Drosophila melanogaster, Insulin-like peptide7-producing neurons are wired with Insulin-producing cells. We found that the former cells regulate the latter to facilitate larval development at high temperatures, and to regulate systemic Insulin signaling in adults feeding on calorie-rich food lacking dietary yeast. Our results demonstrate a role for neuronal innervation of Insulin-producing cells important for fruit flies to survive unfavorable environmental conditions.
Irritant Inhalation Evokes P Wave Morphological Changes in Spontaneously Hypertensive Rats via Reflex Modulation of the Autonomic Nervous System
Authors: Hooper J. Shane , Taylor-Clark Thomas E.
Published: July 2021

Irritant inhalation is associated with increased incidence of atrial fibrillation (AF) and stroke. Irritant inhalation acutely regulates cardiac function via autonomic reflexes. Increases in parasympathetic and sympathetic reflexes may increase atrial susceptibility to ectopic activity and the initiation of arrhythmia such as AF. Both age and hypertension are risk factors for AF. We have shown that irritant-evoked pulmonary–cardiac reflexes are remodeled in spontaneously hypertensive (SH) rats to include a sympathetic component in addition to the parasympathetic reflex observed in normotensive Wistar-Kyoto (WKY) rats. Here, we analyzed P wave morphology in 15-week old WKY and SH rats during inhalation of the transient receptor potential ankyrin 1 agonist allyl isothiocyanate (AITC). P Wave morphology was normal during vehicle inhalation but was variably modulated by AITC. AITC increased RR intervals (RRi), PR intervals, and the P Wave duration. In SH rats only, AITC inhalation increased the occurrence of negative P waves. The incidence of AITC-evoked negative P waves in SH rats was dependent on RRi, increasing during bradycardic and tachycardic cardiac cycles. Inhibition of both parasympathetic (using atropine) and sympathetic (using atenolol) components of the pulmonary–cardiac reflex decreased the incidence of negative P waves. Lastly, the probability of evoking a negative P Wave was increased by the occurrence of preceding negative P waves. We conclude that the remodeled irritant-evoked pulmonary–cardiac reflex in SH rats provides a substrate for altered P Wave morphologies. These are likely ectopic atrial beats that could provide a trigger for AF initiation in structurally remodeled atria.
Base Editing in Plants: Applications, Challenges, and Future Prospects
Authors: Azameti Mawuli K. , Dauda Wadzani Palnam
Published: July 2021

The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology. This technology has attracted global attention and has been employed in functional analysis studies in crop plants. Since many important agronomic traits are confirmed to be determined by single-nucleotide polymorphisms, improved crop varieties could be developed by the programmed and precise conversion of targeted single bases in the genomes of plants. One novel genome editing approach which serves for this purpose is base editing. Base editing directly makes targeted and irreversible base conversion without creating double-strand breaks (DSBs). This technology has recently gained quick acceptance and adaptation because of its precision, simplicity, and multiplex capabilities. This review focuses on generating different base-editing technologies and how efficient they are in editing nucleic acids. Emphasis is placed on the exploration and applications of these base-editing technologies to enhance crop production. The review also highlights the drawbacks and the prospects of this new technology.
The GhSWEET42 Glucose Transporter Participates in Verticillium dahliae Infection in Cotton
Authors: Sun Mengxi , Zhang Zhiqiang , Ren Zhongying , Wang Xingxing , Sun Wenjie , Feng Hongjie , Zhao Junjie , Zhang Fei , Li Wei , Ma Xiongfeng , Yang Daigang
Published: July 2021

The SWEET (sugars will eventually be exported transporter) proteins, a family of sugar transporters, mediate sugar diffusion across cell membranes. Pathogenic fungi can acquire sugars from plant cells to satisfy their nutritional demands for growth and infection by exploiting plant SWEET sugar transporters. However, the mechanism underlying the sugar allocation in cotton plants infected by Verticillium dahliae, the causative agent of Verticillium wilt, remains unclear. In this study, observations of the colonization of cotton roots by V. dahliae revealed that a large number of conidia had germinated at 48-hour post-inoculation (hpi) and massive hyphae had appeared at 96 hpi. The glucose content in the infected roots was significantly increased at 48 hpi. On the basis of an evolutionary analysis, an association analysis, and qRT-PCR assays, GhSWEET42 was found to be closely associated with V. dahliae infection in cotton. Furthermore, GhSWEET42 was shown to encode a glucose transporter localized to the plasma membrane. The overexpression of GhSWEET42 in Arabidopsis thaliana plants led to increased glucose content, and compromised their resistance to V. dahliae. In contrast, knockdown of GhSWEET42 expression in cotton plants by virus-induced gene silencing (VIGS) led to a decrease in glucose content, and enhanced their resistance to V. dahliae. Together, these results suggest that GhSWEET42 plays a key role in V. dahliae infection in cotton through glucose translocation, and that manipulation of GhSWEET42 expression to control the glucose level at the infected site is a useful method for inhibiting V. dahliae infection.
Nonverbal Social Sensing: What Social Sensing Can and Cannot Do for the Study of Nonverbal Behavior From Video
Authors: Renier Laetitia Aurelie , Schmid Mast Marianne , Dael Nele , Kleinlogel Emmanuelle Patricia
Published: July 2021

The study of nonverbal behavior (NVB), and in particular kinesics (i.e., face and body motions), is typically seen as cost-intensive. However, the development of new technologies (e.g., ubiquitous sensing, computer vision, and algorithms) and approaches to study social behavior [i.e., social signal processing (SSP)] makes it possible to train algorithms to automatically code NVB, from action/motion units to inferences. Nonverbal social sensing refers to the use of these technologies and approaches for the study of kinesics based on video recordings. Nonverbal social sensing appears as an inspiring and encouraging approach to study NVB at reduced costs, making it a more attractive research field. However, does this promise hold? After presenting what nonverbal social sensing is and can do, we discussed the key challenges that researchers face when using nonverbal social sensing on video data. Although nonverbal social sensing is a promising tool, researchers need to be aware of the fact that algorithms might be as biased as humans when extracting NVB or that the automated NVB coding might remain context-dependent. We provided study examples to discuss these challenges and point to potential solutions.
Assessing Two Dimensions of Interpersonal Trust: Other-Focused Trust and Propensity to Trust
Authors: Zhang Ming
Published: July 2021

One’s propensity to trust others and others’ trustworthiness are two important aspects of interpersonal trust. Both theory and research suggest that it is possible to distinguish between an individual’s propensity to trust (one’s “trustingness” or the extent to which one feels able to trust others) and their other-focused trust (the extent to which one feels that others are worthy of our trust). However, there is as yet no measure that distinguishes between these two components of trust. In three studies, we examined the psychometrics of a proposed two-dimensional measure of trust that encompasses propensity to trust and other-focused trust components. To test discriminant validity, we also administered measures of personality, personal self-esteem, social capital, propensity to like people, perceived social support, as well as general and personal beliefs in a just world. Factor analyses supported the proposed two-factor model for the new trust measure. Further analyses supported the difference between these measures.
The Building Blocks of Child Bilingual Code-Mixing: A Cross-Corpus Traceback Approach
Authors: Quick Antje Endesfelder , Hartmann Stefan
Published: July 2021

This paper offers an inductive, exploratory study on the role of input and individual differences in the early code-mixing of bilingual children. Drawing on data from two German-English bilingual children, aged 2–4, we use the traceback method to check whether their code-mixed utterances can be accounted for with the help of constructional patterns that can be found in their monolingual data and/or in their caregivers' input. In addition, we apply the traceback method to check whether the patterns used by one child can also be found in the input of the other child. Results show that patterns found in the code-mixed utterances could be traced back to the input the children receive, suggesting that children extract lexical knowledge from their environment. Additionally, tracing back patterns within each child was more successful than tracing back to the other child's corpus, indicating that each child has their own set of patterns which depends very much on their individual input. As such, these findings can shed new light on the interplay of the two developing grammars in bilingual children and their individual differences.
Suicide Exposure in a Polymediated Age
Authors: Bell Jo , Westoby Christopher
Published: July 2021

With growing evidence that media plays a vital role in shaping public understanding of suicidality and influencing behaviours, media portrayals of suicidality have for some time been the focus of suicide prevention efforts. Traditional media has changed, and now exists alongside and within an instantaneous, interactive sharing of information created and controlled by anyone; the way most people use it today incorporates a wide variety of online communication media. Polymedia describes media communication as both a product and process, where anyone can contribute and act as producers, consumers, audiences, and critics. In a “Polymediated age,” media exposure becomes much more complex. To understand how media exposure to suicide influences and impacts on others, it is important to take into account the communicative ecology of media technologies and the different interactions we can have with them. We researched the effects of this type of exposure by conducting in-depth interviews with a purposive sample of individuals who have lived experience and/or knowledge of suicide exposure via polymediated communication in the aftermath of a suicide. Using thematic analysis, our data demonstrates how exposure to suicide has become more complex as a result of new communicative media technology: it can be both a gift and a curse, difficult to distinguish, predict or control. Polymedia has the power to determine new forms of narrative and new forms of behaviour that on the one hand can provide support and prevention efforts, while on the other hand can promote conflict and cast an adverse influence on suicidal behaviour. Polymedia provides novel affordances for very intimate collective exposure to suicide. Our findings shed important new light on how the interplay between news media and social media has transformed our relationship with the information to which we’re exposed. We highlight important suggestions for those working in suicide prevention to develop (1) media strategies that recognise the multiple ways in which users are exposed and impacted, and (2) mechanisms for a strategic amplification and moderation of specific types of content. Media organisations and users of social media alike can contribute to maximising the beneficial capacity of polymediated exposure to suicide.
Circumscribed Interest Modulates Attention to Eyes in Boys With and Without Autism Spectrum Disorder
Authors: Wang Qiandong , Hoi Sio Pan , Song Ci , Li Tianbi , Lam Cheuk Man , Wang Yuyin , Yi Li
Published: July 2021

Children with autism spectrum disorder (ASD) exhibit abnormal visual attention, such as diminished attention to eyes and enhanced attention to high-autism-interest objects. We tested whether high-autism-interest objects would modulate the attention to eyes in boys with ASD and typically developing (TD) boys. Twenty-two ASD and 22 TD children were presented simultaneously with human eyes and high/low-autism-interest objects (HAI/LAI) while their eye movements were recorded. We found that visual preference for eyes was influenced by competing objects in children with and without ASD. Specifically, both children with and without ASD showed reduced overall and first looking preference when eyes were paired with HAI objects relative to LAI objects. Children with ASD also showed reduced sustained viewing preference to the eyes after first looking at the eyes and late looking preference to the eyes after first looking at the objects in the HAI condition than the LAI condition, but these effects were absent in the TD group. Our study not only helps us understand some factors that impact attention to eyes, but also has implications for interventions aiming at improving eye contact in children with ASD.
Adherence to Traditional Chinese Postpartum Practices and Postpartum Depression: A Cross-Sectional Study in Hunan, China
Authors: Guo Pengfei , Xu Dong , Liew Zeyan , He Hua , Brocklehurst Peter , Taylor Beck , Zhang Chao , Jin Xin , Gong Wenjie
Published: July 2021

Background: The relationship between adherence to traditional Chinese postpartum practices (known as “doing-the-month”) and postpartum depression (PPD) remains unknown. Practices including restrictions on diet, housework and social activity, personal hygiene, and cold contact, could introduce biological, psychological, and socio-environmental changes during postpartum.

Methods: The cross-sectional study included 955 postpartum women in obstetric clinics in Hunan Province of China between September 2018 to June 2019. Thirty postpartum practices were collected by a self-report online structured questionnaire. Postpartum depression symptoms were assessed by the Chinese version of the Edinburgh Postnatal Depression Scale (EPDS). Multivariable linear regression was used to estimate the differences in EPDS scores according to adherence to postpartum practices. Firth's bias-reduced logistic regression was employed to analyze the binary classification of having PPD symptoms (EPDS ≥ 10).

Results: Overall, both moderate and low adherence to postpartum practices appeared to be associated with higher EPDS scores (adjusted difference 1.07, 95% CI 0.20, 1.94 for overall moderate adherence; and adjusted difference 1.72, 95% CI 0.84, 2.60 for overall low adherence). In analyses by practice domain, low adherence to housework-related and social activity restrictions was associated with having PPD symptoms compared with high adherence (OR 1.61, 95% CI 1.07, 2.43).

Conclusions: Low adherence to traditional Chinese postpartum practices was associated with higher EPDS scores indicating PPD symptoms, especially in the domain of housework-related and social activity restrictions. Psychosocial stress and unsatisfactory practical support related to low adherence to postpartum practices might contribute to PPD. Longitudinal study and clinical assessment would be needed to confirm these findings.
Dynamic Changes in Community Deprivation of Access to Urban Green Spaces by Multiple Transport Modes
Authors: Cheng Shunqi , Liao Zhiqiang , Zhu Yu
Published: July 2021

Urban green spaces (UGSs) improve the quality of life of urban inhabitants. With the acceleration of urbanization and changes in traffic networks, it remains unclear whether changes in the distribution of UGSs can satisfy the needs of all inhabitants and offer equal services to inhabitants from different socioeconomic backgrounds. This study addresses this issue by analyzing dynamic changes in UGS accessibility in 2012, 2016, and 2020 for inhabitants of the central urban area of Fuzhou in China at the community level. The study introduces multiple transportation modes for an accessibility estimation based on a framework using the two-step floating catchment area method and examines the dynamic changes in community deprivation of UGS accessibility using Kernel regularized least squares, a machine learning algorithm. The results demonstrate that spatial disparities of UGS accessibility exist among the multi-transport modes and vary with time. Communities with high accessibility to UGSs by walking are scattered around the urban area; for accessibility by cycling, the high accessibility regions expand and surround the regions with low accessibility in the core urban areas, forming a semi-enclosed spatial pattern. However, the core urban spatial orientation of UGS accessibility by public transit demonstrates a reverse trend to the above two modes. The spatial pattern of UGS accessibility also varies over time, and the growth rate of accessibility slightly declined during the study period. Furthermore, the increase in UGS accessibility tended to slow from 2016–2020 compared with 2012–2016, and the trend toward equality was also erratic. The degree of deprivation for communities first weakened and was then aggravated, corresponding to the slowdown in the growth rate of accessibility, leading to the persistence existence of social inequality. Moreover, significant deprivation mainly exists among less educated people or those using the cycling and integrated travel modes. Although public transport is developing, deprived communities, such as communities with large proportion of older people, have experienced a decline in access to UGSs by public transport. Based on these findings, the study proposes a policy framework for the balanced distribution of UGSs as part of urbanization.
Coronavirus Disease-2019 Survival in Mexico: A Cohort Study on the Interaction of the Associated Factors
Authors: Márquez-González Horacio , Méndez-Galván Jorge F. , Reyes-López Alfonso , Klünder-Klünder Miguel , Jiménez-Juárez Rodolfo , Garduño-Espinosa Juan , Solórzano-Santos Fortino
Published: July 2021

The pandemic caused by the new coronavirus Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is currently affecting more than 200 countries. The most lethal clinical presentation is respiratory insufficiency, requiring attention in intensive care units (ICU). The most susceptible people are over 60 years old with comorbidities. The health systems organization may represent a transcendental role in survival.

Objective: To analyze the correlation of sociodemographic factors, comorbidities and health system organization variables with survival in cases infected by SARS-CoV-2 during the first 7 months of the pandemic in Mexico.

Methods: The cohort study was performed in a health system public basis from March 1st to September 30th, 2020. The included subjects were positive for the SARS-CoV-2 test, and the target variable was mortality in 60 days. The risk variables studied were: age, sex, geographic distribution, comorbidities, health system, hospitalization, and access to ICU. Bivariate statistics (X2-test), calculation of fatality rates, survival analyses and adjustment of confusing variables with Cox proportional-hazards were performed.

Results: A total of 753,090 subjects were analyzed, of which the 52% were men. There were 78,492 deaths (10.3% of general fatality and 43% inpatient). The variables associated with a higher risk of hospital mortality were age (from 60 years onwards), care in public sectors, geographic areas with higher numbers of infection and endotracheal intubation without management in the ICU.

Conclusions: The variables associated with a lower survival in cases affected by SARS-CoV-2 were age, comorbidities, and respiratory insufficiency (with endotracheal intubation without care in the ICU). Additionally, an interaction was observed between the geographic location and health sector where they were treated.
Information Distribution in Multi-Robot Systems: Generic, Utility-Aware Optimization Middleware
Authors: Barciś Michał , Barciś Agata , Tsiogkas Nikolaos , Hellwagner Hermann
Published: July 2021

This work addresses the problem ofwhatinformation is worth sending in a multi-robot system under generic constraints, e.g., limited throughput or energy. Our decision method is based on Monte Carlo Tree Search. It is designed as a transparent middleware that can be integrated into existing systems to optimize communication among robots. Furthermore, we introduce techniques to reduce the decision space of this problem to further improve the performance. We evaluate our approach using a simulation study and demonstrate its feasibility in a real-world environment by realizing a proof of concept in ROS 2 on mobile robots.
Scalable Object Detection for Edge Cloud Environments
Authors: Hector Rory , Umar Muhammad , Mehmood Asif , Li Zhu , Bhattacharyya Shuvra
Published: July 2021

Object detection is an important problem in a wide variety of computer vision applications for sustainable smart cities. Deep neural networks (DNNs) have attracted increasing interest in object detection due to their potential to provide high accuracy detection performance in challenging scenarios. However, DNNs involve high computational complexity and are therefore challenging to deploy under the tighter resource constraints of edge cloud environments compared to more resource-abundant platforms, such as conventional cloud computing platforms. Moreover, the monolithic structure of conventional DNN implementations limits their utility under the dynamically changing operational conditions that are typical in edge cloud computing. In this paper, we address these challenges and limitations of conventional DNN implementation techniques by introducing a new resource-adaptive scheme for DNN-based object detection. This scheme applies the recently-introduced concept of elastic neural networks, which involves the incorporation of multiple outputs within intermediate stages of the neural network backbone. We demonstrate a novel elastic DNN design for object detection, and we show how other methods for streamlining resource requirements, in particular network pruning, can be applied in conjunction with the proposed elastic network approach. Through extensive experiments, we demonstrate the ability of our methods to efficiently trade-off computational complexity and object detection accuracy for scalable deployment.
A Best-Fit Framework and Systematic Review of Asymmetric Gameplay in Multiplayer Virtual Reality Games
Authors: Rogers Katja , Karaosmanoglu Sukran , Wolf Dennis , Steinicke Frank , Nacke Lennart E.
Published: July 2021

Increasingly, virtual reality (VR) design and research leverages gameplay asymmetries, flattening discrepancies of interface, abilities, information or other aspects between players. A common goal is to induce social interactions that draw players without head-mounted displays into a shared game world. Exploring these asymmetries resulted in many artifacts, creating an innovative yet disparate research landscape that showcases points for improvement in coverage of the field and theoretical underpinnings. In this article, we present a literature review of asymmetry in multiplayer VR games, using a framework synthesis method to assess the field through a lens of existing literature on asymmetries in gameplay. We provide an overview of this emerging subfield and identify gaps and opportunities for future research. Moreover, we discuss how research artifacts address prior theoretical work and present a “best fit” framework of asymmetric multiplayer VR games for the community to build upon.
The Global Session Metric Score (GSMs): A Modified Session-Specific Exertional Index
Authors: Gardner Hanna M. , Scheck Andrew W. , Cone John R. , Berry Nathaniel T. , Wideman Laurie
Published: July 2021

Monitoring session training load to optimize the training stress that drives athlete adaptation and subsequent performance, is fundamental to periodization and programming. Analyzing the internal load experienced by the individual in response to the external load prescribed by coaching staff is crucial to avoid overtraining and optimize training adaptation. Subjective measures provide more information regarding individual training load, as heart rate measures alone do not account for collisions, eccentric muscle actions, muscle soreness, weather conditions, or accumulated training loads, which are paramount to the athlete experience. However, the current subjective metric for interpreting session training load (sRPE) is poorly shaped to the athlete's global response to the whole session, often showing poorer correlations to heart rate (HR) measures during intermittent or high-intensity activity. This study introduces a new metric, the Global Session Metric Score (GSMs), which creates a symmetrical relation between the verbal descriptor and numeric values, as well as more applicable session-specific verbal descriptors for the highest level of exertion. Twenty-four D1 male college soccer field players (age: 20.5 +/– 1.42) wore HR monitors and reported GSMs for all practices and games within an entire season. Linear regression with 10-fold cross validation was used to test the relation between GSMs with B-TRIMP and E-TRIMP, respectively. These models demonstrate good performance with consistency and reliability in the estimation of GSMs to predict both B-TRIMP (R2 = 0.75–0.77) and E-TRIMP (R2 = 0.76–0.78). The findings show promise for the GSMs index as a reliable means for measuring load in both training and matches during a high-intensity intermittent team sport. Future studies should directly compare GSMs to the existing sRPE scale within a controlled laboratory setting and across various other sports. GSMs provides coaches and clinicians a simple and cost-effective alternative to heart rate monitors, as well as a proficient measure of internal training load experienced by the individual.
Short-Term Effects of Sacubitril/valsartan on Echocardiographic Parameters in Dogs With Symptomatic Myxomatous Mitral Valve Disease
Authors: Saengklub Nakkawee , Pirintr Prapawadee , Nampimoon Thanida , Kijtawornrat Anusak , Chaiyabutr Narongsak
Published: July 2021

Background and Objective:Sacubitril/valsartan (SV) is an angiotensin receptor-neprilysin inhibitor that works by inhibiting the neprilysin enzyme as well as blocking angiotensin receptors. The benefits of using SV in congestive heart failure patients has been demonstrated in several clinical trials; however, limited data are available for dogs with heart failure. The aim of this study was to investigate the short-term effects of SV in comparison with ramipril in the standard therapy of symptomatic dogs suffering from myxomatous mitral valve disease (MMVD).

Methods:In this prospective, randomized, single-blind study, 21 dogs with MMVD stage C were randomly assigned to received SV (20 mg/kg orally twice a day) or ramipril (0.125 mg/kg, orally once a day) in addition to pimobendan and furosemide. Echocardiography, electrocardiography, blood pressure, N-terminal pro-B-type natriuretic peptide (NT-proBNP), and urinary aldosterone per creatinine ratio were obtained at baseline (D0) and at follow-up (4 weeks).

Results:When comparing the percent change from baseline between groups, the left atrium to aortic root ratio (LA/Ao) and left ventricular internal diameter diastole normalized to body weight (LVIDDN) were significantly reduced in the SV group (P< 0.001 andP< 0.01, respectively). The end-diastolic volume index (EDVI), end-systolic volume index (ESVI), and stroke volume were lower in the SV group (P< 0.001,P< 0.05, andP< 0.01, respectively). No changes were observed between groups for NTproBNP, blood pressure, ECG parameters, and urinary aldosterone per creatinine ratio.

Conclusion:The current study suggested that the short-term effects of SV can reverse myocardial remodeling, as inferred from several echocardiographic indices (i.e., the reduction in LA/Ao, LVIDDN, EDVI and ESVI) in dogs with MMVD stage C. These findings would support the use of SV in clinically symptomatic heart failure in dogs.
Similar Supergraph Search Based on Graph Edit Distance
Authors: Yamada Masataka , Inokuchi Akihiro
Published: July 2021

Subgraph and supergraph search methods are promising techniques for the development of new drugs. For example, the chemical structure of favipiravir—an antiviral treatment for influenza—resembles the structure of some components of RNA. Represented as graphs, such compounds are similar to a subgraph of favipiravir. However, the existing supergraph search methods can only discover compounds that match exactly. We propose a novel problem, called similar supergraph search, and design an efficient algorithm to solve it. The problem is to identify all graphs in a database that are similar to any subgraph of a query graph, where similarity is defined as edit distance. Our algorithm represents the set of candidate subgraphs by a code tree, which it uses to efficiently compute edit distance. With a distance threshold of zero, our algorithm is equivalent to an existing efficient algorithm for exact supergraph search. Our experiments show that the computation time increased exponentially as the distance threshold increased, but increased sublinearly with the number of graphs in the database.
Osmolyte Accumulation and Sodium Compartmentation Has a Key Role in Salinity Tolerance of Pistachios Rootstocks
Authors: Behzadi Rad Pegah , Roozban Mahmoud Reza , Karimi Soheil , Ghahremani Reza , Vahdati Kourosh
Published: July 2021

Physio-biochemical responses of pistachio varieties including Pistacia vera L. ‘Ghazvini’ (GH), P. vera ‘Ghermez-Pesteh’ (GP) and P. atlantica subsp. mutica (M) were assessed under salt stress to understand the common mechanisms of salt tolerance in two popular Pistacia species. In the experiment, half-sib seedlings of the varieties were subjected to high (100 mM) and severe (200 mM) levels of NaCl-induced salinity for 90 days. Growth, physiological, biochemical and ionic parameters in the roots and shoots of plants were measured in the experiment. Salinity markedly declined plant growth, and increased the number of necrotic leaves (NL) and leaf abscission. In terms of physiological responses, salinity reduced the relative water content (RWC), membrane stability index (MSI) and the concentrations of photosynthetic pigments, but increased carbohydrates and proline content in the leaves. MSI of the leaves was positively correlated with the concentrations of anthocyanins and carotenoids. Salinity increased sodium content in root and shoot tissues of the plants, and decreased potassium concentration and K/Na ratio. Among the rootstocks, GH had better performance on all parameters. Despite the high concentration of Na+ and low K/Na ratio in the shoots, the lowest number of NL was found in GH under both salinity levels. The results indicated that salt tolerance in GH was most likely related to compartmentation of Na+ ions. Finally, accumulation of osmolytes and sodium compartmentation were considered to be the most important mechanisms in the salt tolerance of pistachio rootstocks.
What Happened in That Pit? An Archaeozoological and GIS Approach to Study an Accumulation of Animal Carcasses at the Roman Villa of Vilauba (Catalonia)
Authors: Colominas Lídia , Castanyer Pere , Frigola Joan , Tremoleda Joaquim
Published: July 2021

Some of the deposits of animal remains documented throughout prehistory and history are clearly something other than ordinary waste from meat consumption. For the Roman period and based on their characteristics, these assemblages have been classified as butchery deposits, raw material deposits, deposits created for the hygienic management and disposal of animal carcasses, or ritual deposits. However, some are difficult to classify, and the parameters that define each of them are not clear. Here, we present a unique deposit from the Roman villa of Vilauba (Catalonia). A total of 783 cattle remains were found in an irregular-shaped 187 m2 pit originally dug to extract the clay used in the construction of the villa walls around the third quarter of the 1st century AD. The application of a contextual taphonomy approach, with the integration of archaeozoological variables, stratigraphy and context, and a GIS analysis, allowed us to document the nature and formation of this singular assemblage. It consisted of the carcasses of 14 cattle individuals from which the meat had been removed to take advantage of it by preserving it. Therefore, the parameters that characterise the refuse of this activity are presented here as a baseline for other studies.
Characterization of PAHs Trapped in the Soot from the Combustion of Various Mediterranean Species
Authors: Leroy-Cancellieri Valérie , Cancellieri Dominique , Leoni Eric
Published: July 2021

Climate change causes more frequent and destructive wildfires even transforming them into megafire. Moreover, all biomass fires produce emissions of carbon compounds in the form of soot to the atmosphere with a significant impact on the environment and human health. Indeed, the soot is causing the formation of PAHs from (a) the high temperature thermal alteration of natural product precursors in the source organic matter and (b) the recombination of molecular fragments in the smoke. However, these molecules are known to have carcinogenic effects on human health. It is therefore interesting to quantify the 16 PAHs concentration extracted from soot emitted in open diffusion flame of biomass combustion. To achieve this objective, an analytical method developed for the study of kerosene combustion has been adapted for soot from biomass. This new method allowed to quantify the 16 PAHs defined as priority pollutants by the US EPA for their carcinogenic mutagenic effect and on human health.
Computerized Cognitive Training (CCT) versus Yoga Impact on 12 Month Post Intervention Cognitive Outcome in Individuals with Mild Cognitive Impairment
Authors: Phatak Vaishali S. , Smith Glenn E. , Locke Dona , Shandera-Ochsner Anne , Dean Pamela M. , Ball Colleen , Gutierrez Gianna , Chandler Melanie J.
Published: July 2021

There is currently limited and mixed evidence for the cognitive benefits of Computerized Cognitive Training (CCT) and yoga in persons with Mild Cognitive Impairment (pwMCI). The objective of this study was to investigate the benefit of computerized cognitive training (CCT) vs. physical (yoga) intervention on cognitive abilities. Participants in this study were part of the larger Mayo Clinic’s Healthy Action to Benefit Independence and Thinking (HABIT) program comparative effectiveness trial. The HABIT program is designed for pwMCI and their care partner and consists of five behavioral interventions: CCT, Memory Support System-Calendar (MSS-Calendar), wellness education, support groups, and yoga. The subtractive study design randomly withheld one of the interventions for a total of five study arms. Longitudinal mixed-effects regression models were used to investigate the hypothesis that CCT and yoga has a greater positive impact on psychomotor and basic attention abilities at 12 months post-intervention as compared to the other HABIT interventions. Findings showed CCT had a positive impact compared to yoga on the Cogstate psychomotor/attention composite at 12 months post-intervention (ES = 0.54; unadjusted p value = 0.007, adjusted p value = 0.021). The impact of yoga or combining CCT with yoga did not show statistically significant improvement. Continued CCT practice at home showed further benefit on psychomotor/attention at 12 months post-intervention. There was no significant benefit of CCT or yoga on Cogstate learning/working memory composite.
Dyslexics’ Fragile Oculomotor Control Is Further Destabilized by Increased Text Difficulty
Authors: Ward Lindsey M. , Kapoula Zoi
Published: July 2021

Dyslexic adolescents demonstrate deficits in word decoding, recognition, and oculomotor coordination as compared to healthy controls. Our lab recently showed intrinsic deficits in large saccades and vergence movements with a Remobi device independent from reading. This shed new light on the field of dyslexia, as it has been debated in the literature whether the deficits in eye movements are a cause or consequence of reading difficulty. The present study investigates how these oculomotor problems are compensated for or aggravated by text difficulty. A total of 46 dyslexic and 41 non-dyslexic adolescents’ eye movements were analyzed while reading L’Alouette, a dyslexia screening test, and 35 Kilos D’Espoir, a children’s book with a reading age of 10 years. While reading the more difficult text, dyslexics made more mistakes, read slower, and made more regressive saccades; moreover, they made smaller amplitude saccades with abnormal velocity profiles (e.g., higher peak velocity but lower average velocity) and significantly higher saccade disconjugacy. While reading the simpler text, these differences persisted; however, the difference in saccade disconjugacy, although present, was no longer significant, nor was there a significant difference in the percentage of regressive saccades. We propose that intrinsic eye movement abnormalities in dyslexics such as saccade disconjugacy, abnormal velocity profiles, and cognitively associated regressive saccades can be particularly exacerbated if the reading text relies heavily on word decoding to extract meaning; increased number of regressive saccades are a manifestation of reading difficulty and not a problem of eye movement per se. These interpretations are in line with the motor theory of visual attention and our previous research describing the relationship between binocular motor control, attention, and cognition that exists outside of the field of dyslexia.
Glioblastoma: Emerging Treatments and Novel Trial Designs
Authors: Di Nunno Vincenzo , Franceschi Enrico , Tosoni Alicia , Gatto Lidia , Lodi Raffaele , Bartolini Stefania , Brandes Alba Ariela
Published: July 2021

Management of glioblastoma is a clinical challenge since very few systemic treatments have shown clinical efficacy in recurrent disease. Thanks to an increased knowledge of the biological and molecular mechanisms related to disease progression and growth, promising novel treatment strategies are emerging. The expanding availability of innovative compounds requires the design of a new generation of clinical trials, testing experimental compounds in a short time and tailoring the sample cohort based on molecular and clinical behaviors. In this review, we focused our attention on the assessment of promising novel treatment approaches, discussing novel trial design and possible future fields of development in this setting.
A Spatial Quantitative Systems Pharmacology Platform spQSP-IO for Simulations of Tumor–Immune Interactions and Effects of Checkpoint Inhibitor Immunotherapy
Authors: Gong Chang , Ruiz-Martinez Alvaro , Kimko Holly , Popel Aleksander S.
Published: July 2021

Quantitative systems pharmacology (QSP) models have become increasingly common in fundamental mechanistic studies and drug discovery in both academic and industrial environments. With imaging techniques widely adopted and other spatial quantification of tumor such as spatial transcriptomics gaining traction, it is crucial that these data reflecting tumor spatial heterogeneity be utilized to inform the QSP models to enhance their predictive power. We developed a hybrid computational model platform, spQSP-IO, to extend QSP models of immuno-oncology with spatially resolved agent-based models (ABM), combining their powers to track whole patient-scale dynamics and recapitulate the emergent spatial heterogeneity in the tumor. Using a model of non-small-cell lung cancer developed based on this platform, we studied the role of the tumor microenvironment and cancer–immune cell interactions in tumor development and applied anti-PD-1 treatment to virtual patients and studied how the spatial distribution of cells changes during tumor growth in response to the immune checkpoint inhibition treatment. Using parameter sensitivity analysis and biomarker analysis, we are able to identify mechanisms and pretreatment measurements correlated with treatment efficacy. By incorporating spatial data that highlight both heterogeneity in tumors and variability among individual patients, spQSP-IO models can extend the QSP framework and further advance virtual clinical trials.
Transcription/Replication Conflicts in Tumorigenesis and Their Potential Role as Novel Therapeutic Targets in Multiple Myeloma
Authors: Dutrieux Laure , Lin Yea-Lih , Lutzmann Malik , Rodriguez Raphaël , Cogné Michel , Pasero Philippe , Moreaux Jérôme
Published: July 2021

Plasma cells (PCs) have an essential role in humoral immune response by secretion of antibodies, and represent the final stage of B lymphocytes differentiation. During this differentiation, the pre-plasmablastic stage is characterized by highly proliferative cells that start to secrete immunoglobulins (Igs). Thus, replication and transcription must be tightly regulated in these cells to avoid transcription/replication conflicts (TRCs), which could increase replication stress and lead to genomic instability. In this review, we analyzed expression of genes involved in TRCs resolution during B to PC differentiation and identified 41 genes significantly overexpressed in the pre-plasmablastic stage. This illustrates the importance of mechanisms required for adequate processing of TRCs during PCs differentiation. Furthermore, we identified that several of these factors were also found overexpressed in purified PCs from patients with multiple myeloma (MM) compared to normal PCs. Malignant PCs produce high levels of Igs concomitantly with cell cycle deregulation. Therefore, increasing the TRCs occurring in MM cells could represent a potent therapeutic strategy for MM patients. Here, we describe the potential roles of TRCs resolution factors in myelomagenesis and discuss the therapeutic interest of targeting the TRCs resolution machinery in MM.