bluuebunny/crossref_metadata_2025 huggingface dataset:
Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels
Authors: Sevimli Gulsah , Smith Matthew , Caglar Tuba Akgul , Bilir Şükriye , Secilmis Melike , Altun Hamza , Yigit Esra , Yang Fan , Malli Roland , Öztürk Gürkan , Mann Giovanni , Eroglu Emrah
Published: February 2022

Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation and pericellular oxygen levels in cultured cells and their downstream effects on metalloproteins has been less explored. This study investigates the functionality of geNOps - an iron-containing metalloprotein and NO biosensor - in cultured HEK293T epithelial and EA.hy926 endothelial cells adapted to standard room air (18 kPa O2) or physiological normoxia (5 kPa O2). We show that cells in culture require iron supplementation to activate the metalloprotein geNOps and demonstrate that cells adapted to physiological normoxia require significantly lower iron to achieve geNOps signals comparable to those measured in cells adapted to hyperoxia. This study establishes an essential role for recapitulating oxygen levels in vivo and uncovers a previously unrecognized requirement for ferrous iron supplementation under standard cell culture conditions to achieve geNOps functionality.
Phytotelm to assess bacterial multi-resistance to the antibiotics in a deep tropical forest
Authors: Sanchez-Vargas Stephanny , Vargas-Jiménez Paula , Vega-Corrales Luis , Pérez-Molina Junior Pastor
Published: February 2022

Paradoxically, the use of antibiotics has resulted in the proliferation of resistant microorganisms in environments where they were not thought to be likely to appear. In this context, this research aimed to evaluate the phytotelmata as a matrix to detect the presence of bacterial strains with antibiotic resistance in the deep understory of tropical cloud forest and in a nearby rural town. The study was carried out in the Santa Elena Cloud Forest Reserve and the urban zone of the Santa Elena town, only 10 km. A compounded sample of phytotelm water was collected from 10—15 bromeliads and heliconia plants by site. Gram-negative bacterial strains found were evaluated to antibiotic susceptibility tests (13 antibiotics). In both sites, five strains presented multi-resistance to more than three antibiotics. Overall, antibiotics possessing cell wall synthesis inhibitor mechanisms were the most common with resistance for both sites followed by those acting as protein synthesis inhibitors. NMDS and PERMANOVA revealed that both environments had a similar response to antibiotic resistance (P > 0.05). The detection of bacterial multi-resistance to antibiotics in understory phytotelm water could become a quick tool to determine the degree of exposure of natural ecosystems to anthropogenic effects. This research opens the discussion of the potential dangers of the appearance of bacterial antibiotic multi-resistance in natural ecosystems, whose future impact is uncertain.
The Relationship between Perceived Stress and Overall Well-being during the COVID-19 epidemic: A moderated mediation model
Authors: Yang Minqi , Guo Hanxiao , Jie Chen , Guo Xicheng , Tian Kexin , Qu Chunyu
Published: February 2022

Objective: To explore the relationship between perceived stress and well-being of Chinese people and the mechanism underlying the association during the COVID-19 epidemic. Methods: An online survey was conducted in the general population to (N = 498) using the Chinese Perceived Stress Scale, General Well-Being Schedule, Short Dark Triad, and Safety Questionnaire. Results: (a) perceived stress was significantly negatively correlated with overall well-being and sense of security, (b) sense of security played a partial mediating role between perceived stress and overall well-being, (c) the Dark Triad played a moderation role in the path from perceived stress to sense of security. These results provide insight into the subjective well-being and underlying psychological processes associated with the perceived stress. The implication of these findings were interpreted and discussed.
A Real-Time Location System Design for Production Facilities Working under COVID-19 Pandemic Precautions
Authors: Kır Sena
Published: February 2022

By reason of the COVID-19 pandemic, essential digital transformations are taking place in many areas of business life. Although the most important one of these transformations is due to the widespread use of the remote working model, the production sector does not have the opportunity to switch to such a model completely. Therefore, it is inevitable to maintain social distance to prevent the spread of COVID-19 while working in production facilities. In this study, a real-time location system (RTLS) model is proposed to keep track of social distance in production facilities and to ensure occupational health safety (OHS) at the same time. Since the social distance rule is essential for every production facility, the most important feature of the proposed system is that it can easily be integrated into the standard personnel tracking system in almost every enterprise. Besides, the proposed RTLS is designed as an efficient system based on ultra-wideband and radio-frequency identification, which can operate as a closed-loop monitoring system within itself. An adequately installed RTLS can monitor the position of employees in real-time and provides to intervene in the situation instantly when necessary. In case of a violation of social distance or a situation against OHS, it can be prevented instantly by the proposed system. It is also a useful model in the management of emergencies.
Effects of Reduced Gag Cleavage Efficiency on HIV-1 Gag-Pol Package
Authors: Lin Yi-Ru , Chu Shih-Ming , Yu Fu-Hsien , Huang Kuo-Jung , Wang Chin-Tien
Published: February 2022

Background
HIV-1 pol, which encodes enzymes required for virus replication, is initially translated as a Gag-Pol fusion protein. Gag-Pol is incorporated into virions via interactions with Gag precursor Pr55gag. Protease (PR) embedded in Gag-Pol mediates the proteolytic processing of both Pr55gag and Gag-Pol during or soon after virus particle release from cells. Since efficient Gag-Pol viral incorporation depends on interaction with Pr55gag via its N-terminal Gag domain, the prevention of premature Gag cleavage may alleviate Gag-Pol packaging deficiencies associated with cleavage enhancement from PR.
Results
We engineered PR cleavage-blocking Gag mutations with the potential to significantly reduce Gag processing efficiency. Such mutations may mitigate the negative effects of enhanced PR activation on virus assembly and Gag-Pol packaging due to an RT dimerization enhancer or leucine zipper dimerization motif. When co-expressed with Pr55gag, we noted that enhanced PR activation resulted in reduced Gag-Pol cis or trans incorporation into Pr55gag particles, regardless of whether or not Gag cleavage sites within Gag-Pol were blocked.
Conclusion
Our data suggest that the amount of HIV-1 Gag-Pol or Pol viral incorporation is largely dependent on virus particle production, and that cleavage blocking in the Gag-Pol N-terminal Gag domain does not exert significant impacts on Pol packaging.
The Mechanism of Cartilage Regeneration by Buffy Coat and the Pre-clinical Study
Authors: Song Guo , Gurung Kumar , Fu ShouYong , Jin GuangWen , Jin Longhao
Published: February 2022

BackgroundAutologous bone marrow buffy coat transplantation possesses obvious advantages in the therapy of large cartilage defects and osteoarthritis. However, there is no definite research on the specific effective components of bone marrow buffy coat and its mechanism of cartilage regeneration. Moreover, as the crucial cartilage regenerative cell in bone marrow buffy coat, mesenchymal stem cells(MSCs) are difficult to fix onto the damaged cartilage area without damaging the subchondral bone. We assessed the effect of using hyaluronic acid(HA) as a liquid scaffold mixed with autologous bone marrow buffy coat to fix cartilage defect.Methods and MaterialsWe extracted the bone marrow from the anterior superior iliac crest of the white New Zealand rabbit, centrifuged to obtain a buffy coat, and analyzed the components of buffy coat by ELISA. Buffy coat+HA group, MSC+HA group, MSC+TGF-β+HA group were cultured in vitro and observed by staining. In addition, we made damage to the femoral condyle of rabbits, and divided them into groups: HA group, buffy coat group, buffy coat with HA group. Each group was assessed for cartilage regeneration by visual observation, histological and immunohistochemical analysis at 4 weeks and 8 weeks, and biochemical analysis at 8 weeks postoperatively. One-way ANOVA and LSD were used for statistic analysis.ResultsBuffy coat have a variety of growth factors, inflammatory factors and anti-inflammatory factors that stimulate the MSCs’ regeneration. Buffy coat can differentiate into cartilage without TGF-β stimulation in vitro. The cartilage regeneration ability of buffy coat and buffy coat+HA is strong, and the combination of buffy coat and liquid scaffold HA can make cartilage formation ability more stable in vivo.ConclusionMSC, multiple growth factors and cytokines in buffy coat synergistically promote cartilage regeneration. Liquid scaffold HA enhances the effect of buffy coat on cartilage attachment and regeneration of cartilage defects.
Subjektive Sichtweisen auf Integration und deren Ausgänge in Sportorganisationen
Authors:   , Weißmann Sarah
Published: February 2022

Der Diskurs über Integration und Integrationsmechanismen fand seinen Beginn in der Migrationsforschung, deren Anfänge bis in die erste Hälfte des 19. Jahrhunderts zurückreichen . Innerhalb dieses Feldes fanden immer wieder Wandlungen der Ausgestaltung des Begriffs der Integration statt, da die theoretischen Konzepte in unterschiedlichen gesellschaftlichen sowie historischen Kontexten entstanden sind bzw. entstehen. Heute existiert eine Vielzahl an theoretischen Perspektiven auf Integration, die eine hohe Heterogenität und auch Interdisziplinarität aufweisen. Generell zeigt sich somit, dass, wenn von Integration gesprochen wird, nicht wirklich klar ist, was unter dem Begriff zu verstehen ist. Die vorliegende Arbeit macht es sich somit zur Aufgabe, den Begriff der Integration und damit verbundene Zielvorstellungen aus Sicht der Akteurinnen und Akteure in Sportorganisationen zu beleuchten. Dafür wird eine Studie im Mixed-Methods-Design durchgeführt, die eine fragebogenbasierte Umfrage, explorative Interviews mit Vereinsvorständen und vertiefende Leitfadeninterviews mit Vereinsmitgliedern umfasst. Ein besonderer Fokus der Gruppenvergleiche liegt dabei auf Personen mit und ohne Migrationshintergrund, um die Perspektive von Personen mit Migrationshintergrund selbst in den Diskurs einbringen zu können. Da sich subjektive Sichtweisen auch aufgrund weiterer Faktoren unterscheiden können, werden außerdem Zusammenhänge zwischen sozialstrukturellen Merkmalen sowie Merkmalen der Vereine und subjektiven Sichtweisen auf Integration untersucht.
Pharmacoeconomic Analysis of Gilteritinib in the Therapy of Adult Patients with Relapsed/Refractory Acute Myeloid Leukemias with FLT3 Mutation
Authors: Kolbin A.S. , Gomon Yuliya Mikhailovna , Balykina Yu.E. , Proskurin M.A.
Published: February 2022

Background. The implementation of new FLT3-targeted drugs in clinical practice has changed the approaches to the management of patients with acute myeloid leukemias (AML) with FLT3 mutation. One of these drugs is gilteritinib, approved by FDA in 2018 as a drug of choice in the therapy of adult patients with relapsed/refractory AML with FLT3 mutation. Aim. To assess the economic feasibility of gilteritinib in the therapy of adult patients with relapsed/refractory AML with FLT3 mutation. Materials & Methods. Pharmacoeconomic modelling was based on Markov and decision-tree models. Incremental cost-effectiveness ratio (ICER) was calculated relative to the efficiency indicator “overall survival”. Its values were compared with those calculated for venetoclax as reference drug, which has been already included in Essential Drug List and used as a first-line drug in this population if high-dose chemotherapy therapy is contraindicated. A Budget Impact Analysis has been made. Results. Gilteritinib proved to be not only more effective in terms of overall survival (median 9.3 vs. 5.6 months), but also more cost-intensive (7,408,108 vs. 1,685,356 rubles a year) compared to the currently used polychemotherapy regimens. However, the difference of ICER with venetoclax was +4.89 % of total costs. The Budget Impact Analysis showed that the total economic load of implementing gilteritinib in clinical practice throughout 3 years will be 8,628,658,505 rubles. Conclusion. Gilteritinib therapy is economically feasible and viable for adult patients with relapsed/refractory AML with FLT3 mutation.
Assessing the role of interatomic position matrix elements in tight-binding calculations of optical properties
Authors: Ibañez-Azpiroz Julen , de Juan Fernando , Souza Ivo
Published: February 2022

We study the role of hopping matrix elements of the position operator
\hat{r}r̂
in tight-binding calculations of linear and nonlinear optical properties
of solids. Our analysis relies on a Wannier-interpolation scheme based
on calculations, which automatically includes matrix elements of
\hat{r}r̂
between different Wannier orbitals. A common approximation, both in
empirical tight-binding and in Wannier-interpolation calculations, is to
discard those matrix elements, in which case the optical response only
depends on the on-site energies, Hamiltonian hoppings, and orbital
centers. We find that interatomic \hat{r}r̂-hopping
terms make a sizeable contribution to the shift photocurrent in
monolayer BC_22N,
a covalent acentric crystal. If a minimal basis of
p_zpz
orbitals on the carbon atoms is used to model the band-edge response,
even the dielectric function becomes strongly dependent on those
terms.
CARBOXITERAPIA E ULTRASSOM NA REDUÇÃO DA LIPODISTROFIA ABDOMINAL: UMA REVISÃO INTEGRATIVA
Authors: Borba Juliana Amorim , Lima Beatriz Moreira
Published: February 2022

O objetivo do presente estudo é verificar a eficácia da carboxiterapia e ultrassom na redução da lipodistrofia abdominal em mulheres. Uma das disfunções estéticas que mais causam incômodo é o acumulo de gordura na região abdominal, que pode estar associada à genética, maus hábitos alimentares e sedentarismo. A pesquisa evidenciou que a carboxiterapia e o ultrassom são técnicas benéficas na diminuição da gordura localizada. A carboxiterapia é um procedimento não invasivo que através da aplicação de CO2 auxilia na queima de gordura, ele também promove o aumento do fluxo sanguíneo local, eleva a produção de colágeno e melhora o aspecto tegumentar. O ultrassom é outro procedimento que atua diretamente na redução da lipodistrofia, é um recurso terapêutico que possui alto poder de penetração, tendo sucesso em causar modificações nos tecidos.
A qualitative study on resource barriers facing scaled container-based sanitation service chains
Authors: Ferguson Charlie , Mallory Adrian , Anciano Fiona , Russell Kory , Valladares Hellen del Rocio Lopez , Riungu Joy , Parker Alison
Published: February 2022

Container-based sanitation (CBS) is an increasingly recognised form of off-grid sanitation provision appropriate for impoverished urban environments. To ensure a safely managed and sustainable service, a managing organisation must implement a service chain that performs robustly and cost-effectively, even with an expanding customer base. These ‘CBS operators’ adopt varying approaches to achieve this objective. Following research including interviews with representatives from six current CBS operators, this paper presents a generalised diagrammatic model of a CBS service chain and discusses the three broad thematic challenges currently faced by these organisations. Supplying cover material is a universal problem with hidden challenges when taking advantage of freely available resources. There is no universally applicable approach for the efficient collection of faecal waste despite the high labour costs of waste collection. The best strategy depends on the CBS operator's overall expansion strategy and the location of fixed features within the served community. Although CBS is technically well-suited to being turned into new products within the circular economy, in practice, this requires a diverse range of skills from CBS operators and is hampered by slow growth in other organic waste recovery services and unhelpful regulation.
Effect of Silver Nanoparticles (AgNPs) on Candida albicans, Candida
dubliniensis and Candida guilliermondii
Authors: Madani Mahboobeh , Shakib Pegah , Masoumizadeh Mahnaz , Fatahian Soheil
Published: February 2022

Background:

Candida species are the most important fungal pathogens and are sometimes
considered the fourth most common cause of infection in hospitals. Today, research needs to be conducted
on the antifungal effect of silver nanoparticles (AgNPs) due to toxicity, side effects, and drug
interactions of antifungal chemical drugs.

Objective:

Therefore, this study was conducted to evaluate the antifungal activity of AgNPs on Candida
albicans, Candida dubliniensis, and Candida guilliermondii.

Methodology:

Antifungal activity of AgNPs on Candida albicans, Candida dubliniensis, and Candida
guilliermondii was assessed by agar and macrodilution diffusion methods in an in-vitro investigation.
Structural changes were investigated by scanning electron microscope (SEM). Then, the obtained data
were evaluated by SPSS statistical software.

Results:

Based on the results, the mean diameter of growth inhibition halos by AgNPs was equal to
20, 20.2, and 40.7mm for Candida albicans, Candida dubliniensis, and Candida guilliermondii, respectively.
The minimum inhibitory concentrations (MIC) equal 62.50, 31.25, and 15.62 mg/ml for Candida
albicans, Candida dubliniensis, and Candida guilliermondii, respectively. The minimum fungicidal
concentrations (MFC) were equal to 125, 62.50, and 31.25 mg/ml for Candida albicans, Candida
dubliniensis, and Candida guilliermondii, respectively.

Conclusion:

Our results revealed that AgNPs inhibit the growth of Candida albicans, Candida
dubliniensis, and Candida guilliermondii. SEM observations also showed that NPs disrupted cell
membrane/wall. Changes in yeast levels from smooth to uneven were also observed.
HSC and miRNA Regulation with Implication for Foetal Haemoglobin
Induction in Beta Haemoglobinopathies
Authors: Okeke Chinwe , Silas Ufele , Nnodu Obiageli , Clementina Odoh
Published: February 2022

Sickle cell disease (SCD) is one of the most common haemoglobinopathies worldwide, with up
to 70 % of global SCD annual births occurring in sub-Saharan Africa. Reports have shown that 50 to 80
% of affected children in these countries die annually. Efforts geared towards understanding and controlling
HbF production in SCD patients could lead to strategies for effective control of globin gene expression
and therapeutic approaches that could be beneficial to individuals with haemoglobinopathies. Hemopoietic
stem cells (HSCs) are characterized by a specific miRNA signature in every state of differentiation.
The role of miRNAs has become evident both in the maintenance of the “stemness” and in the early
induction of differentiation by modulation of the expression of the master pluripotency genes and during
early organogenesis. miRNAs are extra regulatory mechanisms in hematopoietic stem cells (HSCs) via
influencing transcription profiles together with transcript stability. miRNAs have been reported to be used
to reprogram primary somatic cells toward pluripotency. Their involvement in cell editing holds the potential
for therapy for many genetic diseases. This review provides a snapshot of miRNA involvement in
cell fate decisions, haemoglobin induction pathway, and their journey as some emerge prime targets for
therapy in beta haemoglobinopathies.
Angiogenic and Migratory Gene Expression Analysis of Stem Cells From
Exfoliated Deciduous Teeth for Wound Repair Application
Authors: Aziz Nur Syazwani , Azlina Ahmad , Yusop Norhayati
Published: February 2022

Background:

The migration and differentiation of stem cells take place during the reparative
phase of the healing cascade. Chemokine ligands and receptors are the key players in the homing process
during the early stage of capillary morphogenesis. Stem cells from exfoliated deciduous teeth are known
to possess a huge potential benefit for tissue regeneration. However, the gene expression of SHED engaging
in angiogenesis and migratory activity during tissue healing is not fully understood. This study aims
to assess the gene expression of SHED following in-vitro angiogenesis and migratory induction protocol.

Methods:

Scratch test assay was conducted following an angiogenic induction of SHED by supplementation
of EGM-2 and VEGF. For the detection of migratory cell markers, angiogenic markers, and stem cell
markers, RNA samples were extracted on days 1, 3, 7, 10, and 14 after the angiogenic induction in a
transwell chamber, followed by RT-PCR analysis.

Results:

The findings suggested that SHED formed endothelial cells at higher capacity under an immature
state with higher seeding density. SHED undergoing angiogenesis and migratory activity showed elevated
IL-8, CCR1, CXCR4, and CCL28 expression. CCR1 expression significantly increased in the A+M+
group (p<0.05).

Conclusion:

The gene expression of these chemokines, particularly CCR1, which closely represent cellular
migration, suggests the potential use of SHED for cell-based therapy to enhance tissue repair.
Reflexões sobre regências dos verbos ligar e telefonar em Português Europeu e llamar e telefonear em Espanhol Peninsular
Authors: Faria Marcela
Published: February 2022

Thiswork,basedonwrittencorporadata,presentssomethoughtsaboutthepossiblevariationoftheprepositionswiththeverbsligar(‘tocall’)andtelefonar(‘totelephone’),inEuropeanPortuguese,andllamar(‘tocall’)andtelefonear(‘totelephone’)inPeninsularSpanish.Takingintoaccountthecomplexityoftheprepositioncategory,weanalysethediversityoftheconstructionswiththeseverbsandwereflectupondessemantizationasthebase of that variation.
Intra-population variation of body temperature of the lizard Sceloporus anahuacus (Squamata: Phrynosomatidae) in Sierra del Ajusco, Mexico
Authors: Güizado-Rodríguez Martha Anahí , García-Vázquez Uri Omar , Solano-Zavaleta Israel , Maceda-Cruz Rolando Jonathan , Duifhuis-Rivera Christopher
Published: February 2022

We evaluated intra-population variation of body temperature of the Mexican lizard Sceloporus anahuacus (Phrynosomatidae) in Sierra del Ajusco, Mexico. The geographic distribution of this lizard is restricted to the higher parts of the mountains bordering the south of the Mexican Valley in Mexico City. The study of the body temperature of S. anahuacus and its relationship with the environment is important because high elevation taxa are seriously threatened by climate change. For that reason, and compared body temperature between sex, age class, reproductive condition and activity state. Overall, mean body temperature was 26.2 ± 5.5 °C with a range between 9–39 °C. We did not find differences between body temperatures of males and females. However, we found significant differences depending on activity state in both sexes. In females, body temperature did not vary as a function of age class or reproductive status. Instead, we found differences in body temperature between males of different age classes. Juveniles had the highest body temperature, probably due to differential temperature requirements associated with development. On the other hand, body temperature of active and inactive S. anahuacus was strongly associated with environmental temperature, perhaps due to sedentary habits and territorial behavior. Exploring why such variation exists on body temperature regulation may provide information on factors influencing the survivorship of this endemic Mexican lizard.
Coccinélidos (Coleoptera: Coccinellidae) asociados a cítricos del centro-sur de Tamaulipas, México
Authors: Lara Luis Alejandro , Godinez-Cortés Sergio , Torres Reyna Ivonne , Padrón-Torres Eduardo , Reyes José , González-González Felipe Anastacio , Hernández Verónica , González-Nieto Eduardo Daniel , Hernández Torres Hermelindo
Published: February 2022

El presente estudio identifica e ilustra la riqueza de especies de Coccinellidae asociados a cultivos de cítricos en el centro-sur de Tamaulipas, México. La colecta de especímenes se realizó de marzo a octubre de 2020 en intervalos semanales, usando red entomológica, trampas amarillas y revisando manualmente las ramas de los árboles de menor tamaño. Se recolectaron 169 especímenes adultos de la familia Coccinellidae representados en dos subfamilias, 13 géneros y 20 especies. De las 20 especies, 14 ya estaban reportadas y seis son primeros registros para Tamaulipas. Se concluye que el aumento en el número de especies recolectadas en esta región se debe al mayor esfuerzo en el muestreo realizado.
Utilization of Siam Weed leaves (Chromolaena odorata L.) as natural dyes
Authors: Dulmalik Dulmalik , Rosliani Selli , Rosliana Sella
Published: February 2022

Siam Weed leaves can be used as natural dyes since they contain tannin compounds which give a brown color to the dyeing of fabrics. This study was conducted to utilize Siam Weed leaves as a natural dye substitute for synthetic cotton cloth and obtain dye from Siam Weed leaves in liquid form. The material used is a dyed cotton cloth with the extract of Siam Weed leaves. The variation of dipping time was 30 minutes and 13 hours. The types of fixators used were Ferrous Sulfate and Alum solution. The findings showed that the type of fixator can affect the color result of the fabric. The fixed fixator produces a dark green color while the Alum solution produces a yellow color. Based on the results of the dry rub test, the cloth that has been immersed in the dye for 13 hours and fixed by an Alum fixator produces the maximum color fastness value with a staining scale value of 5, 5, 5 (very good). Whereas in the wet rub test, the cloth that has been immersed in the dye for 13 hours and fixed with an Alum solution fixator produces good values with a range of values for the staining scale 4-5, 4-5, 4-5 (good). In the colorfastness test of washing cloth soap soaked for 30 minutes, the best dye fastness with a grayscale value of 4-5, 4-5, 4-5 (good). The type of fixator that has the best colorfastness in the washing test is the Alum fixator.
The characteristics of stability test using Bantak as the main aggregate
Authors: Aqif Mohamad , Haque Md. Masudul , Ahmad Wasiu Akande
Published: February 2022

This study aims to determine the characteristics of the stability test using Bantak as the main material. Fundamental studies in terms of the characteristics of Stability values include Density (Density), Flow (melting), VIM (Void in Mix), VMA (Void in Mineral Aggregate), VFB (Void Filled Bitumen), and Marshall Quotient (MQ). This study uses an experimental test method consisting of different bitumen content variants, respectively, 5%, 5.5%, 6%, 6.5%, and 7%. Each variant consists of three samples of the test object. The research was carried out at the building materials laboratory, Faculty of Engineering, Universitas Negeri Yogyakarta. The implementation stages include inspection of AC 60/70, an inspection of aggregates (fine aggregate and coarse aggregate), an inspection of fillers, and manufacturing mixed specimens and stability tests with various characteristics. The results of the research showed that the average value of stability, density, flow, void in the mix, void mineral aggregate, void filled bitumen, and Marshall quotient was 1156.44 kg, 2.33 gr/cc, 3.07 mm, 5.20 %, 13.92% 60.10% and 377.28 kg/mm. Finally, Bantak aggregate can be used as an alternative in the manufacture of hot asphalt mixtures because the research performance data meet the requirements for accepting Indonesian national standards. Its abundant availability makes Bantak a solution in exploiting Merapi material, which is very influential on the environmental impact and improves residents' economy in the quarry area.
Kesulitan siswa SMA dalam menyelesaikan soal matematika pada materi peluang
Authors: Fitri Annisa , Abadi Agus Maman
Published: February 2022

Penelitian ini bertujuan untuk mendeskripsikan jenis kesulitan siswa SMA dalam menyelesaikan soal matematika pada materi peluang di Kabupaten Indragiri Hilir. Penelitian ini termasuk penelitian survei dengan pendekatan kuantitatif dan kualitatif. Sampel penelitian sebanyak 284 siswa SMA kelas XI Program IPA di Kabupaten Indragiri Hilir, Indonesia, yang berasal dari sepuluh sekolah dengan kategori tinggi, sedang, dan rendah. Sampel penelitian ditentukan menggunakan teknik stratified propotional random sampling. Instrumen yang digunakan berupa tes yang telah divalidasi oleh ahli (expert judgement). Kesalahan siswa dijustifikasi berdasarkan Newman’s errors categories. Hasil penelitian menunjukkan bahwa jenis kesulitan siswa dalam menyelesaikan soal matematika pada materi peluang yaitu kesulitan memahami masalah (comprehension errors) sebanyak 64,1%; kesulitan melakukan transformasi (transformations errors) sebanyak 71,1%; kesulitan keterampilan proses (processing skill errors) sebanyak 89,4%; dan kesulitan menarik kesimpulan (encoding errors) sebanyak 94%. AbstractThis study aimed to describe the types of difficulties high school students in solving mathematics problems on probability material in Indragiri Hilir Regency. This study includes a survey with quantitative and qualitative approaches. The research sample was 284 eleventh-grade high school students in the science program on Indragiri Hilir Regency, Indonesia, from ten schools with high, medium, and low categories. The research sample was determined using a stratified proportional random sampling technique. The instrument used was a test that has been validated by an expert. Students’ errors were justified based on Newman’s errors categories. The results showed that the types of students’ difficulties in solving mathematics problems on the probability material, namely: difficulty in understanding the problem (comprehension errors) as many as 64.1%; difficulty in making transformations (transformation errors) as many as 71.1%; difficulty in process skill (processing skill errors) as many as 89.4%; and difficulty in concluding (encoding errors) as many as 94%.
Tahap berpikir geometri siswa SMP berdasarkan teori Van Hiele ditinjau dari keterampilan geometri
Authors: Susanto Sdyoko , Mahmudi Ali
Published: February 2022

Penelitian ini bertujuan untuk mendeskripsikan tahap berpikir geometri siswa SMP menurut teori Van Hiele  ditinjau dari keterampilan geometri.  Jenis penelitian adalah deskriptif menggunakan pendekatan kualitatif. Sampel penelitian adalah sembilan siswa kelas VIII dari tiga SMP Negeri Kota Sampit yang dipilih menggunakan teknik stratified proportional random sampling dan dilanjutkan dengan purposive sampling. Data penelitian dikumpulkan melalui wawancara mendalam terhadap subyek setelah diberikan tes keterampilan geometri. Teknik analisis data yang digunakan adalah kualitatif secara interaktif maju berkelanjutan di antara pengumpulan data, reduksi data, penyajian data, triangulasi data, dan penarikan kesimpulan. Hasil penelitian mengungkapkan bahwa: (1) keterampilan geometri siswa beragam pada tiap tahap berpikir geometri; (2) keterampilan verbal dan keterampilan menggambar merupakan keterampilan yang banyak dikuasai subjek; (3) keterampilan verbal merupakan keterampilan yang paling sulit bagi siswa; (4) tahap berpikir geometri siswa SMP kelas VIII SMP Negeri Sampit adalah 43,6% siswa berada di tahap 0 (visualisasi), 35,1% siswa di tahap 1 (analisis), dan 21,3% siswa di tahap 2 (deduksi informal).AbstractThis study aimed to describe the geometric thinking stages of junior high school students according to van Hiele's theory in terms of geometry skills. This type of research is descriptive using a qualitative approach. The research sample was nine 8th grade students from three public junior high schools in Sampit City, Indonesia, who were selected using a stratified proportional random sampling technique and followed by purposive sampling. Research data were collected through in-depth interviews with subjects after being given a geo­metry skills test. The data analysis technique was an interactively qualitative, continuous process between data collection, data reduction, data presentation, data triangulation, and concluding. The results of the study revealed that: (1) students' geometry skills varied at each stage of geometric thinking; (2) verbal and drawing were the skills that mastered mainly by subjects; (3) verbal skill was the most difficult for students; (4) the geometric thinking stage of students was 43.6% of students in stage 0 (visualization), 35.1% of students in stage 1 (analysis), and 21.3% of students in stage 2 (informal deduction).
High school students’ attitudes toward mathematics and its relation to mathematics learning achievement
Authors: Idris Khairiani , Khazila Khazila , Agustina Agustina , Lisa Lisa
Published: February 2022

Attitudes toward mathematics have been acknowledged among factors related to students’ mathematics learning achievement. The positive attitudes may de­crease when the education level getting higher. This study applied a mixed-method approach to describe students’ attitudes toward mathematics and to analyze the correlation between attitudes toward mathematics and mathematics learning achievement among students in the coastal area of Aceh Province, spe­cifically in Lhokseumawe City and North Aceh Regency. Total of 481 eleventh graders completed attitudes toward mathematics questionnaire online and 19 of them were involved in semi-structured interview. Data of mathematics learning achievement was obtained from students’ scores in their report card of the pre­vious semester. Data analyses were conducted by using Spearman’s correlation and chi-square test. The results of the study revealed that attitudes toward mathematics have a weak negative correlation but significant with mathematics learning achievement. This shows that there is a small percentage of students who have low learning achievement in mathematics tend to have a positive attitude towards mathematics in the high category, while students with high achievement on the contrary. Based on the results of the study, we provide seve­ral suggestions regarding learning mathematics and the direction of further research.
Development of a web-based worksheet with a project-based learning model assisted by GeoGebra
Authors: Muchlis Effie Efrida , Priatna Nanang , Dahlan Jarnawi Afgani
Published: February 2022

Previous studies on the use of GeoGebra in mathematics learning were more focused on implementation and effectiveness in learning outcomes. However, not many studies focus on providing opportunities to students to explore Geo­Gebra to find concepts in plane geometry through developing an application. Therefore, this research and development applied a 4D model to produce a web-based worksheet using project-based learning (PjBL) assisted by GeoGebra that was valid, practical, and effective in the plane geometry course. The web-based worksheet intended to facilitate students to be independent, creative, and able to find the concepts in the plane geometry course through exploration activities using GeoGebra. The results of the data analysis revealed that this research and development produced a web-based worksheet using PjBL assisted by Geo­Gebra that was valid, practical, and effective. The validity of the web-based student worksheet was shown from the validation results explaining that the worksheet was good in terms of content, construction, and language. The prac­ticality of the worksheet was shown from the results of practitioner assessments and student questionnaires, showing that the web-based worksheet was easy to use and attractive. The effectiveness of the worksheet was shown from the formative assessment results with the interpretation that the use of the web-based worksheet could help students understand the content of plane geometry well.
Keefektifan pendekatan STEM berbantuan Wolfram Alpha pada pembelajaran matematika ditinjau dari motivasi dan kemandirian belajar
Authors: Argianti Atin , Andayani Sri
Published: February 2022

Penelitian ini bertujuan untuk mendeskripsikan keefektifan pendekatan STEM berbantuan Wolfram Alpha ditinjau dari motivasi dan kemandirian belajar. Penelitian ini merupakan penelitian eksperimen semu dengan populasi seluruh siswa kelas VIII di salah satu SMP Negeri di Pati, Jawa Tengah, Indonesia. Sampel penelitian ini adalah siswa di satu kelas VIII yang dibagi menjadi 2, yaitu kelas eksperimen (kelompok siswa bernomor absen ganjil) dan kelas kontrol (kelompok siswa bernomor absen genap) dengan banyaknya siswa berturut-turut 16 dan 15 siswa. Data dikumpulkan menggunakan angket motivasi dan kemandirian belajar. Analisis data menggunakan uji t untuk mendeskripsikan keefektifan pendekatan STEM berbantuan Wolfram Alpha ditinjau dari masing-masing variabel. Hasil penelitian menunjukkan bahwa (1) pendekatan STEM berbantuan Wolfram Alpha efektif ditinjau dari motivasi, dan (2) pendekatan STEM berbantuan Wolfram Alpha efektif ditinjau dari kemandirian belajar. Hasil analisis keefektifan pendekatan STEM berbantuan Wolfram Alpha ditinjau dari motivasi ditunjukkan dari nilai t variabel motivasi adalah 4,258 dan nilai signifikan motivasi setelah perlakuan adalah 0,001 kurang dari 0,05. Keefektifan pendekatan STEM berbantuan Wolfram Alpha ditinjau dari kemandirian belajar dibuktikan dari nilai t variabel kemandirian adalah 5,030 dan nilai signifikannya 0,000 kurang dari 0,05.AbstractThis study aims to reveal the effectiveness of Wolfram Alpha-assisted STEM approach in terms of motivation and self-regulated learning. This study was quasi-experimental research. The population were all grade VIII students of a State Junior High School in Pati, Central Java, Indonesia. The sample were students of a class in grade VIII, which is divided into two groups, namely the experimental group (odd-numbered students) and the control group (even-numbered students) with 16 and 15 participating students respectively. The data were collected using questionnaires on motivation and self-regulated learning. The data were analyzed using the t-test to describe the effectiveness of Wolfram Alpha-assisted STEM approach. The result shows that: (1) the Wolfram Alpha-assisted STEM approach is effective in improving motivation; and (2) the Wolfram Alpha-assisted STEM approach is effective in improving self-regulated learning. The results of the analysis of the Wolfram Alpha-assisted STEM approach is effective in terms of motivation with a score of 4.258 and with the significance value of motivation after treatment being 0.001, which is less than 0.05. The Wolfram Alpha-assisted STEM approach is effective in terms of self-regulated learning with a score of 5.030 and with the significance value of 0.000, which is less than 0.05.
Pengembangan model pembelajaran creative problem solving terintegrasi TPACK untuk meningkatkan kemampuan pemecahan masalah
Authors: Waluyo Edy , Nuraini Nuraini
Published: February 2022

Guru perlu menerapkan model pembelajaran yang mampu mengembangkan kemampuan berpikir tingkat tinggi dan kemampuan pemecahan masalah. Penelitian ini bertujuan untuk mengembangkan model pembelajaran Creative Problem Solving (CPS) terintegrasi TPACK untuk mengembangkan dan meningkatkan kemampuan siswa dalam pemecahan masalah. Penelitian ini merupakan penelitian pengembangan dengan model pengembangan Borg Gall. Sampel penelitian ini adalah siswa kelas VII Sekolah Menengah Petama di Lombok Timur yang berjumlah 30 siswa, yang terdiri dari 12 siswa laki laki dan 18 siswa perempuan.  Instrumen yang digunakan untuk mengumpulkan data meliputi pedoman validasi desain pembelajaran CPS terintegrasi TPACK, angket kepraktisan dan tes kemampuan pemecahan masalah pada materi geometri. Analisis data yang digunakan dalam penelitian ini menggunakan analisis deskriptif. Berdasarkan analisis validasi, produk yang dikembangkan termasuk kategori valid yang ditunjukkan oleh  skor rata-rata validasi produk oleh  3 ahli yaitu ahli bahasa, ahli materi dan ahli teknologi pembelajaran  berturut turut 79,4; 79,9; dan 80,8. Begitu pula dari segi kepraktisan, produk yang dikembangkan juga tergolong praktis dengan rata-rata skor kepraktisan 82,67. Produk juga tergolong efektif dengan persentase keefektifan sebesar 86,67% . Produk yang dikembangkan mempunyai efek potensial dalam kategori tinggi untuk meningkatkan kemampuan siswa dalam pemecahan masalah matematika dengan N-Gain sebesar 0,81.AbstractTeachers need to apply teaching models that enable students to develop high order thinking skills and student problem-solving skills. This research aims to develop TPACK's integrated creative problem solving (CPS) teaching model to improve students' ability to solve problems. This research was developmental research applying Borg Gall development model. The sample was 30 grade VII students of a State Junior High School, in Lombok Timur, Indonesia, consisting of 12 male and 18 female students. The instruments used to collect the data include TPACK integrated CPS learning design validation guidelines, practicality questionnaires, and troubleshooting tests on geometric materials. The data analysis used descriptive analysis. Based on the validation analysis, the developed model was in a valid category as indicated by the average score of product validation by three experts: a linguist, materials expert, and learning technology expert, with scores of 79.4, 79.9, and 80.8 consecutively. Similarly, in terms of practicality, the developed model is also relatively practical with an average practicality score of 82.67. The model is also classified as effective, with an effectiveness percentage of 86.67%. It has a potential effect in the high category to improve students' ability to solve mathematic problems with an n-gain of 0.81.
International Technologies on Prevention and Treatment of Neurological and Psychiatric Diseases: Bibliometric Analysis of Patents
Authors: Zheng Fuhao , Wang Ling , Zeng Zhaonan , Wu Siying
Published: February 2022

Background

Neurological and psychiatric disorders are serious and expensive global public health problems. Therefore, exploring effective intervention technologies plays an important role in improving patients’ clinical symptoms and social functions, as well as reducing medical burden.

Objective

The aim of this study is to analyze and summarize the key new technologies and innovative development trends witnessed globally for neurological illness and psychiatric disorders by mining the relevant patent data.

Methods

A bibliometric analysis was conducted on patent applications, priority countries, main patentees, hot technologies, and other patent information on neurological and psychiatric disorders, revealing the current situation along with the trend of technology development in this field.

Results

In recent years, inventions and innovations related to neurological and psychiatric diseases have become very active, with China being the largest patent priority country. Of the top patent holders, Visicu (headquartered in the United States) is the leader. The distribution of patent holders in China remains relatively scattered, with no monopoly organization at present. Global technologies on neurological illness and psychiatric disorders are mainly concentrated around A61B (diagnosis, surgery, and identification).

Conclusions

This paper analyzed and summarized the key new technologies and global innovative development trends of neurological and psychiatric diseases by mining the relevant patent data, and provides practical references and research perspectives for the prevention and treatment of the aforesaid diseases.
Understanding the Potential of Mental Health Apps to Address Mental Health Needs of the Deaf and Hard of Hearing Community: Mixed Methods Study
Authors: Borghouts Judith , Neary Martha , Palomares Kristina , De Leon Cinthia , Schueller Stephen M , Schneider Margaret , Stadnick Nicole , Mukamel Dana B , Sorkin Dara H , Brown Dakota , McCleerey-Hooper Shannon , Moriarty Gloria , Eikey Elizabeth V
Published: February 2022

Background

Mental health concerns are a significant issue among the deaf and hard of hearing (D/HH) community, but community members can face several unique challenges to accessing appropriate resources.

Objective

The aim of this study was to investigate the mental health needs of the D/HH community and how mental health apps may be able to support these needs.

Methods

A total of 10 members of the D/HH community participated in a focus group and survey to provide their perspectives and experiences. Participants were members of the Center on Deafness Inland Empire team, which comprises people with lived experience as members of and advocates for the D/HH community.

Results

Findings identified a spectrum of needs for mental health apps, including offering American Sign Language and English support, increased education of mental health to reduce stigma around mental health, direct communication with a Deaf worker, and apps that are accessible to a range of community members in terms of culture, resources required, and location.

Conclusions

These findings can inform the development of digital mental health resources and outreach strategies that are appropriate for the D/HH community.
Big Data Health Care Innovations: Performance Dashboarding as a Process of Collective Sensemaking (Preprint)
Authors: van Elten Hilco J , Sülz Sandra , van Raaij Erik M , Wehrens Rik
Published: February 2022

UNSTRUCTURED

Big data is poised to revolutionize health care, and performance dashboards can be an important tool to manage big data innovations. Dashboards show the progress being made and provide critical management information about effectiveness and efficiency. However, performance dashboards are more than just a clear and straightforward representation of performance in the health care context. Instead, the development and maintenance of informative dashboards can be more productively viewed as an interactive and iterative process involving all stakeholders. We refer to this process as dashboarding and reflect on our learnings within a large European Union–funded project. Within this project, multiple big data applications in health care are being developed, piloted, and scaled up. In this paper, we discuss the ways in which we cope with the inherent sensitivities and tensions surrounding dashboarding in such a dynamic environment.

INTERNATIONAL REGISTERED REPORT

RR2-10.2196/16779