bluuebunny/crossref_metadata_2025 huggingface dataset:
Deformation Behavior of Transient Liquid-Phase Sintered Cu-Solder-Resin Microstructure for Die-Attach
Authors: Tatsumi Hiroaki , Yamaguchi Hiroshi , Matsuda Tomoki , Sano Tomokazu , Kashiba Yoshihiro , Hirose Akio
Published: August 2019

We have proposed a low-temperature bonding technology utilizing the sintering of Cu particles with transient liquid-phase of Sn-based solder, called transient liquid-phase sintering (TLPS), as a die-attach solution for high-temperature power modules. A copper-intermetallic compound-resin (Cu-IMC-resin) microstructure, which consists of Cu particles connected with Cu–Sn intermetallic compounds (IMCs) partially filled with polyimide resin, is obtained by the pressureless TLPS process at 250 °C for 1 min using a novel Cu-solder-resin composite as the bonding material in a nitrogen atmosphere. Macro- and micro-deformation properties of the unique microstructure of the TLPS Cu-IMC-resin are evaluated by finite element analysis using a three-dimensional image reconstruction model. The macroscopic computational uniaxial tensile tests of the Cu-IMC-resin model reveal that the utilization of the IMCs and the addition of the easily-deformable resin facilitates the temperature-stability and low-stiffness of the mechanical properties. The microstructure exhibits a significantly low homogenized Young’s modulus (11 GPa). Microscopic investigations show that the local stresses are broadly distributed on the IMC regions under uniaxial macroscopic tensile displacement, indicating highly reliable performance of the joint within a specific macroscopic strain condition. Numerical and experimental investigations demonstrate the excellent thermal cyclic reliability of die-attached joints between silicon carbide chips and directly bonded copper substrate.
Development of the Dyskinesia Impairment Mobility Scale to Measure Presence and Severity of Dystonia and Choreoathetosis during Powered Mobility in Dyskinetic Cerebral Palsy
Authors: Bekteshi Saranda , Konings Marco , Nica Ioana Gabriela , Gakopoulos Sotirios , Vanmechelen Inti , Aerts Jean-Marie , Hallez Hans , Monbaliu Elegast
Published: August 2019

The majority of individuals with dyskinetic cerebral palsy cannot use powered mobility with a joystick, due to the lack of manual abilities by the severe presence of dystonia and choreoathetosis. Reliable measurements of these movement disorders is indispensable for good evaluation towards evidence–based insights during powered mobility. This study aimed to develop and assess the Dyskinesia Impairment Mobility Scale (DIMS), a video–based tool to measure presence and severity of dystonia and choreoathetosis during powered mobility. DIMS was measured for the neck and arms region during five mobility tasks. Interrater reliability, test–retest reliability, internal consistency and concurrent validity of the DIMS were assessed. Interrater reliability coefficients ranged between 0.68 and 0.87 for the total DIMS, and the dystonia and choreoathetosis subscales. Test–retest reliability was moderate to excellent (range 0.51–0.93) while Cronbach’s alpha was good (range 0.69–0.81) for the total scale and subscale scores. Concurrent validity showed during mobility tasks significant correlations with rest postures in the arm region, and with requested but voluntary activity in the neck region. The DIMS reliably measures the presence and severity of the movement disorders during powered mobility, increasing insights into the underlying mechanisms of independent mobility. This scale may therefore be a promising tool to evaluate mobility training.
Testing Procedure of Unmanned Aerial Vehicles (UAVs) Trajectory in Automatic Missions
Authors: Ćwiąkała Paweł
Published: August 2019

This paper describes an experimental test campaign while using an Unmanned Aerial Vehicle (UAV) and measuring the obtained UAV positions during different flight tasks and in different operative conditions. A new test procedure has been presented and tested for different devices in various weather conditions. This paper describes and analyses the measurements of the flight trajectory of the UAV that was performed with the use of a robotic total station (RTS), as compared to the design data and the data recorded in the internal memory of the UAV. Five different test tasks have been conducted. The obtained results have allowed for the assessment of the correctness of task performance as compared to the design and to determine the flying accuracy of the entire UAV set. The proposed set of tasks can be successfully utilised to control the correctness of operation of various types of UAVs and it may be implemented as a universal test to verify the algorithms optimising take-offs and landings, test flights of the objects, as well as flight planning in various terrain and weather conditions, which will increase the safety of the flights while using UAVs.
Numerical Analysis and Dynamic Response of Optimized Composite Cross Elliptical Pressure Hull Subject to Non-Contact Underwater Blast Loading
Authors: Helal Mahmoud , Huang Huinan , Fathallah Elsayed , Wang Defu , Mokbel ElShafey Mohamed , Ali Mohamed A. E. M.
Published: August 2019

Among the most important problems confronted by designers of submarines is to minimize the weight, increase the payload, and enhance the strength of pressure hull in order to sustain the hydrostatic pressure and underwater explosions (UNDEX). In this study, a Multiple Intersecting Cross Elliptical Pressure Hull (MICEPH) subjected to hydrostatic pressure was first optimized to increase the payload according to the design requirements. Thereafter, according to the optimum design results, a numerical analysis for the fluid structure interaction (FSI) phenomena and UNDEX were implemented using nonlinear finite element code ABAQUS/Explicit. The propagation of shock waves through the MICEPH was analyzed and the response modes (breathing, accordion and whipping) were discussed. Furthermore, the acceleration, displacement and failure index time histories at different locations were presented. The results showed that the greatest acceleration occurred in the athwart direction, followed by the vertical and longitudinal directions. Additionally, the first bubble pulse has a major effect on athwart acceleration. Moreover, the analysis can be effectively used to predict and calculate the failure indices of pressure hull. Additionally, it provides an efficient method that reasonably captures the dynamic response of a pressure hull subjected to UNDEX.
Fault Data Detection of Traffic Detector Based on Wavelet Packet in the Residual Subspace Associated with PCA
Authors: Li Xiaolu , Zhang Xi , Zhang Peng , Zhu Guangyu
Published: August 2019

To improve the accuracy and efficiency of fault data identification of traffic detectors is crucial in order to decrease the probability of unexpected failures of the intelligent transportation system (ITS). Since convolutional fault data recognition based on traffic flow three-parameter law has a poor capability for multiscale of fault data, PCA (principal component analysis) is adopted for traffic fault data identification. This paper proposes the fault data detection models based on the PCA model, MSPCA (multiscale principal component analysis) model and improved MSPCA model, respectively. In order to improve the recognition rate of traffic detectors’ fault data, the improved MSPCA model combines the wavelet packet energy analysis and PCA to achieve traffic detector data fault identification. On the basis of traditional MSPCA, wavelet packet multi-scale decomposition is used to get detailed information, and principal component analysis models are established on different scale matrices, and fault data are separated by wavelet packet energy difference. Through case analysis, the feasibility verification of traffic flow data identification method is carried out. The results show that the improved method proposed in this paper is effective for identifying traffic fault data.
Stiffness Modulus and Marshall Parameters of Hot Mix Asphalts: Laboratory Data Modeling by Artificial Neural Networks Characterized by Cross-Validation
Authors: Baldo Nicola , Manthos Evangelos , Miani Matteo
Published: August 2019

The present paper discusses the analysis and modeling of laboratory data regarding the mechanical characterization of hot mix asphalt (HMA) mixtures for road pavements, by means of artificial neural networks (ANNs). The HMAs investigated were produced using aggregate and bitumen of different types. Stiffness modulus (ITSM) and Marshall stability (MS) and quotient (MQ) were assumed as mechanical parameters to analyze and predict. The ANN modeling approach was characterized by multiple layers, the k-fold cross validation (CV) method, and the positive linear transfer function. The effectiveness of such an approach was verified in terms of the coefficients of correlation ( R ) and mean square errors; in particular, R values were within the range 0.965 – 0.919 in the training phase and 0.881 – 0.834 in the CV testing phase, depending on the predicted parameters.
Seismic Vulnerability Analysis of Structure Subjected to Uneven Foundation Settlement
Authors: Bao Chao , Xu Fangze , Chen Gang , Ma Xiaotong , Mao Mingjie , Zhang Shangrong
Published: August 2019

Uneven foundation settlement is one of the common engineering problems in a collapsible loess area. In order to study the influence of uneven foundation settlement on the seismic performance of a structure, the incremental dynamic analysis (IDA) method is used to analyze the seismic vulnerability of the steel structure frame. The differences in the seismic response of the structure in relation to uneven foundation settlement are analyzed. The influences of uneven foundation settlement quantities and various areas of uneven settlement on the seismic response of the structure are discussed. On this basis, the relationship between ground motion intensity and structural failure probability is studied, by changing the magnitude of seismic acceleration peaks. Compared with the unsettled structure, the internal force redistribution of the structure caused by uneven foundation settlement is one of the causes of earthquake damage for some components. The uneven foundation settlement located at the corner of the plane of the structure is likely to cause more serious earthquake damage to the structure. Uneven settlement will cause an increase in storey drift. With the increase in settlement, the seismic damage of the superstructure will be aggravated.
Performance Assessment of Grid Forming Converters Using Different Finite Control Set Model Predictive Control (FCS-MPC) Algorithms
Authors: Alhasheem Mohammed , Blaabjerg Frede , Davari Pooya
Published: August 2019

Finite control set model predictive control (FCS-MPC) methods in different power electronic applications are gaining considerable attention due to their simplicity and fast dynamics. This paper introduces an assessment of the two-level three-phase voltage source converter (2L-VSC) utilizing different MPC schemes with and without a modulation stage. In order to perform such a comparative evaluation, 2L-VSC efficiency and total harmonics distortion of the voltage (THDv) have been investigated, when considering a linear load. The results demonstrate the performance of different MPC algorithms through an experimental verification on a Danfoss converter, and a set of analyses have been studied using the PLECS and MATLAB/SIMULINK together. It can be concluded that a comparable performance is achieved by using conventional MPC (CMPC), improved MPC (IMPC), periodic MPC (PMPC), and MPC scheme with modulator (M 2 PC) controllers. The assessment is critical to classify the strategies as mentioned earlier according to their efficiency. Furthermore, it gives a thorough point of view on which algorithm is suitable for the grid-forming applications.
Situational Ecumenism: The Architecture of Jewish Student Centers on American University Campuses
Authors: Kargon Jeremy
Published: August 2019

Since the start of the 20th century, the presence of Jewish students on American university campuses required accommodation of their religious practices. Jewish activities, including prayer, took place in existing campus buildings designed for other purposes. Eventually, at some universities, facilities were built to serve Jewish religious and social needs. These Jewish Student Centers, which include worship spaces yet are typologically different from synagogues, generally have to accommodate the diverse religious streams that characterize Jewish life in the United States. To do so, both architects and Jewish organizations have adapted the idea of ecumenism, by which related sects seek unity through fellowship and dialogue, not doctrinal agreement. Three examples—at Yale, Duke University, and the University of California San Diego—demonstrate differently the situational ecumenism at the core of their designs. These buildings, and other Jewish Student Centers elsewhere, make visible the intersection between American collegiate and Jewish religious values, variously defined.
Measuring Regional Atmospheric CO2 Concentrations in the Lower Troposphere with a Non-Dispersive Infrared Analyzer Mounted on a UAV, Ogata Village, Akita, Japan
Authors: Chiba Takashi , Haga Yumi , Inoue Makoto , Kiguchi Osamu , Nagayoshi Takeshi , Madokoro Hirokazu , Morino Isamu
Published: August 2019

We have developed a simple measuring system prototype that uses an unmanned aerial vehicle (UAV) and a non-dispersive infrared (NDIR) analyzer to detect regional carbon dioxide (CO2) concentrations and obtain vertical CO2 distributions. Here, we report CO2 measurement results for the lower troposphere above Ogata Village, Akita Prefecture, Japan (about 40° N, 140° E, approximately −1 m amsl), obtained with this UAV system. The actual flight observations were conducted at 500, 400, 300, 200, 100, and 10 m above the ground, at least once a month during the daytime from February 2018 to February 2019. The raw CO2 values from the NDIR were calibrated by two different CO2 standard gases and high-purity nitrogen (N2) gas (as a CO2 zero gas; 0 ppm). During the observation period, the maximum CO2 concentration was measured in February 2019 and the minimum in August 2018. In all seasons, CO2 concentrations became higher as the flight altitude was increased. The monthly pattern of observed CO2 changes is similar to that generally observed in the Northern Hemisphere as well as to surface CO2 changes simulated by an atmospheric transport model of the Japan Meteorological Agency. It is highly probable that these changes reflect the vegetation distribution around the study area.
VAPOR: A Visualization Package Tailored to Analyze Simulation Data in Earth System Science
Authors: Li Shaomeng , Jaroszynski Stanislaw , Pearse Scott , Orf Leigh , Clyne John
Published: August 2019

Visualization is an essential tool for analysis of data and communication of findings in the sciences, and the Earth System Sciences (ESS) are no exception. However, within ESS, specialized visualization requirements and data models, particularly for those data arising from numerical models, often make general purpose visualization packages difficult, if not impossible, to use effectively. This paper presents VAPOR: a domain-specific visualization package that targets the specialized needs of ESS modelers, particularly those working in research settings where highly-interactive exploratory visualization is beneficial. We specifically describe VAPOR’s ability to handle ESS simulation data from a wide variety of numerical models, as well as a multi-resolution representation that enables interactive visualization on very large data while using only commodity computing resources. We also describe VAPOR’s visualization capabilities, paying particular attention to features for geo-referenced data and advanced rendering algorithms suitable for time-varying, 3D data. Finally, we illustrate VAPOR’s utility in the study of a numerically- simulated tornado. Our results demonstrate both ease-of-use and the rich capabilities of VAPOR in such a use case.
Metastasis of Cancer Stem Cells Developed in the Microenvironment of Hepatocellular Carcinoma
Authors: Afify Said M. , Hassan Ghmkin , Osman Amira , Calle Anna Sanchez , Nawara Hend M , Zahra Maram Hussein , EL-Ghlban Samah , Mansour Hager , Alam Md Jahangir , Abu Quora Hagar A , Du Juan , Seno Akimasa , Iwasaki Yoshiaki , Seno Masaharu
Published: August 2019

Metastasis develops when cancer cells spread from the primary site of a malignant tumor to the surrounding and distant tissues, and it is the most critical problem in cancer treatment. Our group developed cancer stem cells (CSCs) from induced pluripotent stem cells (iPSCs) in the presence of a conditioned medium (CM) of cancer-derived cells. The CSCs were characterized by the formation of malignant tumors in vivo, followed by metastasis. In this study, CSCs converted from mouse iPSCs in the presence of CM from hepatocellular carcinoma (HCC) cell line Huh7 cells. These converted cells (miPS-Huh7cm cells) were established as the metastatic cells. The generated CSCs were injected into the liver or spleen of nude mice. Almost one month after transplantation, the tumors were excised, and the primary cultured cells derived from the malignant tumors and metastatic nodules were evaluated by stemness and metastatic markers to compare their differences. The miPS-Huh7cm cells exhibited metastatic potential, and efficiently formed malignant tumors with lung and/or liver lesions in vivo, whereas the injected miPS formed teratoma. The primary cultured cells derived from the malignant tumors and metastatic nodules sustained the expression of stemness markers, such as Nanog, Klf4 and c-Myc, and acquired cancer stem markers, such as CD90, CD44 and ALDH1. Simultaneously, the expression of metastatic markers, such as Slug, Twist1 and vimentin, in primary cells derived from the malignant tumors, was higher than in metastatic nodules. The CSCs derived from iPSCs, forming malignant tumors and displaying high metastasis, will provide a good animal model to study the mechanisms of metastasis.
Biotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379
Authors: Kucera Dan , Novackova Ivana , Pernicova Iva , Sedlacek Petr , Obruca Stanislav
Published: August 2019

The terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 4-hydroxybutyrate (4HB) was produced employing Cupriavidus sp. DSM 19379. Growth in the presence of γ-butyrolactone, ε-caprolactone, 1,4-butanediol, and 1,6-hexanediol resulted in the synthesis of a polymer consisting of 3HB and 4HB monomers. Single and two-stage terpolymer production strategies were utilized to incorporate the 3HV subunit into the polymer structure. At the single-stage cultivation mode, γ-butyrolactone or 1,4-butanediol served as the primary substrate and propionic and valeric acid as the precursor of 3HV. In the two-stage production, glycerol was used in the growth phase, and precursors for the formation of the terpolymer in combination with the nitrogen limitation in the medium were used in the second phase. The aim of this work was to maximize the Polyhydroxyalkanoates (PHA) yields with a high proportion of 3HV and 4HB using different culture strategies. The obtained polymers contained 0–29 mol% of 3HV and 16–32 mol% of 4HB. Selected polymers were subjected to a material properties analysis such as differential scanning calorimetry (DSC), thermogravimetry, and size exclusion chromatography coupled with multi angle light scattering (SEC-MALS) for determination of the molecular weight. The number of polymers in the biomass, as well as the monomer composition of the polymer were determined by gas chromatography.
Serum Apurinic/Apyrimidinic Endodeoxyribonuclease 1 (APEX1) Level as a Potential Biomarker of Cholangiocarcinoma
Authors: Tummanatsakun Doungdean , Proungvitaya Tanakorn , Roytrakul Sittiruk , Limpaiboon Temduang , Wongkham Sopit , Wongkham Chaisiri , Silsirivanit Atit , Somintara Ongart , Sangkhamanon Sakkarn , Proungvitaya Siriporn
Published: August 2019

Diagnostic and/or prognostic biomarkers for cholangiocarcinoma (CCA) are still insufficient with poor prognosis of patients. To discover a new CCA biomarker, we constructed our secretome database of three CCA cell lines and one control cholangiocyte cell line using GeLC-MS/MS. We selected candidate proteins by five bioinformatics tools for secretome analysis. The inclusion criteria were as follows: having predicted signal peptide or being predicted as non-classically secreted protein; together with having no transmembrane helix and being previously detected in plasma and having the highest number of signal peptide cleavage sites. Eventually, apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1) was selected for further analysis. To validate APEX1 as a bio-marker for CCA, serum APEX1 levels of 80, 39, and 40 samples collected from CCA, benign biliary diseases (BBD), and healthy control groups, respectively, were measured using dot blot analysis. The results showed that serum APEX1 level in CCA group was significantly higher than that in BBD or healthy control group. Among CCA patients, serum APEX1 level was significantly higher in patients having metastasis than in those without metastasis. The higher level of serum APEX1 was correlated with the shorter survival time of the patients. Serum APEX1 level might be a diagnostic and prognostic biomarker for CCA.
Sequential Multilingualism and Cognitive Abilities: Preliminary Data on the Contribution of Language Proficiency and Use in Different Modalities
Authors: Boumeester Marlijne , Michel Marije C. , Fyndanis Valantis
Published: August 2019

This exploratory study focuses on sequential bi-/multilinguals (specifically, nonimmigrant young Dutch native speakers who learned at least one foreign language (FL) at or after the age of 5) and investigates the impact of proficiency-based and amount-of-use-based degrees of multilingualism in different modalities (i.e., speaking, listening, writing, reading) on inhibition, disengagement of attention, and switching. Fifty-four participants completed a comprehensive background questionnaire, a nonverbal fluid intelligence task, a Flanker task, and the Trail Making Test. Correlational and regression analyses considering multilingualism related variables and other variables that may contribute to the cognitive abilities under investigation (e.g., years of formal education, socioeconomic status, physical activity, playing video-games) revealed that only proficiency-based degrees of multilingualism impacted cognitive abilities. Particularly, mean FL writing proficiency affected inhibition (i.e., significant positive flanker effect) and L2 listening proficiency influenced disengagement of attention (i.e., significant negative sequential congruency effect). Our findings suggest that only those speakers who have reached a certain proficiency threshold in more than one FL show a cognitive advantage, which, in our sample, emerged in inhibition only. Furthermore, our study suggests that, regarding the impact of proficiency-based degrees of multilingualism on cognitive abilities, for our participants the writing and listening modalities mattered most.
Technological Advances and Trends in Modern High-Rise Buildings
Authors: Szolomicki Jerzy , Golasz-Szolomicka Hanna
Published: August 2019

The purpose of this paper is to provide structural and architectural technological solutions applied in the construction of high-rise buildings, and present the possibilities of technological evolution in this field. Tall buildings always have relied on technological innovations in engineering and scientific progress. New technological developments have been continuously taking place in the world. It is closely linked to the search for efficient construction materials that enable buildings to be constructed higher, faster and safer. This paper presents a survey of the main technological advancements on the example of selected tall buildings erected in the last decade, with an emphasis on geometrical form, the structural system, sophisticated damping systems, sustainability, etc. The famous architectural studios (e.g., for Skidmore, Owings and Merill, Nikhen Sekkei, RMJM, Atkins and WOHA) that specialize, among others, in the designing of skyscrapers have played a major role in the development of technological ideas and architectural forms for such extraordinary engineering structures. Among their completed projects, there are examples of high-rise buildings that set a precedent for future development.
Involvement of the Anterior Commissure in Early Glottic Cancer (Tis-T2): A Review of the Literature
Authors: Hendriksma Martine , Sjögren Elisabeth V.
Published: August 2019

Background: The impact of the anterior commissure (AC) involvement on prognosis in laryngeal cancer remains a topic of discussion with inconsistent results in the literature. This review examines AC involvement as a prognostic factor in patients with early glottic cancer (Tis–T2) treated with radiotherapy or transoral laser microsurgery (TLM). Methods: A systematic literature search was performed. Due to the heterogeneity of the data, no meta-analysis was implemented. Weighted averages were calculated if the appropriate data were extractable. Results: Thirty-four studies on radiotherapy and 23 on TLM fit the inclusion criteria. The majority of studies for both radiotherapy (67.7%) and TLM (75.0%) did not report a significant impact on oncological outcomes. Weighted averages were slightly lower in patients with AC involvement. The two studies that applied a more detailed classification showed a significant impact on the amount of AC involvement. Conclusions: Binary variables (yes/no) for AC involvement lead to inconsistent results. Studies that use more detailed classifications of the AC show that there is a significant impact on the outcome. To further elucidate the role of the AC, detailed stratification of tumors involving the AC need to be investigated in further studies for both treatment modalities.
The Diverse Contributions of Fucose Linkages in Cancer
Authors: Keeley Tyler S. , Yang Shengyu , Lau Eric
Published: August 2019

Fucosylation is a post-translational modification of glycans, proteins, and lipids that is responsible for many biological processes. Fucose conjugation via α(1,2), α(1,3), α(1,4), α(1,6), and O’- linkages to glycans, and variations in fucosylation linkages, has important implications for cancer biology. This review focuses on the roles that fucosylation plays in cancer, specifically through modulation of cell surface proteins and signaling pathways. How L-fucose and serum fucosylation patterns might be used for future clinical diagnostic, prognostic, and therapeutic approaches will be discussed.
Novel High Efficiency Layered Oxide Photocatalyst Li2SnO3 for Rhodamine B and Tetracycline Degradation
Authors: Li Yuanyuan , Wu Meijun , Yang Dingfeng , Zeng Hanlu , Zhang Tao , Shen Jinfeng , Zhang Bin , Li Qiaoqi
Published: August 2019

The use of a layered Li2SnO3 material as an efficient photocatalyst for the degradation of environmental pollutants (Rhodamine B and tetracycline) was investigated. The structure and morphology of the material were characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, and field emission scanning electron microscopy. Optical measurements demonstrated that Li2SnO3 was a UV-light-responsive material with a band gap of 3.71 eV. The maximum kinetic rate constants of photocatalytic degradation of Rhodamine B and tetracycline solutions were 0.0155 min−1 and 0.0406 min−1 · L/mg, respectively, when exposed to UV-light irradiation within 120 min. Trapping experiments demonstrated that holes ( h + ) , hydroxyl radical ( · O H ) and superoxide radical ( · O 2 − ) were the dominant active species during the degradation of Rhodamine B and tetracycline. Theoretical band structure calculations revealed that Li2SnO3 was a direct gap semiconductor with a large m h * / m e * value (4.7) near the band edge. Partial charge density near the top of the valence band indicated that the photocatalytic oxidation reaction occurred largely on the O-2p states. The excellent photocatalytic performance was attributed to the synergistic effect of the layered crystal structure and large m h * / m e * . This work represents an important contribution to the design and optimization of efficient oxide photocatalysts with layered crystal structures for environmental remediation.
Cu0.4Co0.6MoO4 Nanorods Supported on Graphitic Carbon Nitride as a Highly Active Catalyst for the Hydrolytic Dehydrogenation of Ammonia Borane
Authors: Li Junhao , Li Fangyuan , Liao Jinyun , Liu Quanbing , Li Hao
Published: August 2019

As a typical chemical hydride, ammonia borane (AB) has received extensive attention because of its safety and high hydrogen storage capacity. The aim of this work was to develop a cost-efficient and highly reactive catalyst for hydrolyzing AB. Herein, we synthesized a series of CuxCo1–xMoO4 dispersed on graphitic carbon nitride (g-C3N4) to dehydrogenate AB. Among those CuxCo1–xMoO4/g-C3N4 catalysts, Cu0.4Co0.6MoO4/g-C3N4 exhibited the highest site time yield (STY) value of 75.7 m o l H 2 m o l c a t − 1   m i n − 1 with a low activation energy of 14.46 kJ mol−1. The STY value for Cu0.4Co0.6MoO4/g-C3N4 was about 4.3 times as high as that for the unsupported Cu0.4Co0.6MoO4, indicating that the g-C3N4 support plays a crucial role in improving the catalytic activity. Considering its low cost and high catalytic activity, our Cu0.4Co0.6MoO4/g-C3N4 catalyst is a strong candidate for AB hydrolysis for hydrogen production in practical applications.
Dehydrogenation of 2-[(n-Methylcyclohexyl)Methyl]Piperidine over Mesoporous Pd-Al2O3 Catalysts Prepared by Solvent Deficient Precipitation: Influence of Calcination Conditions
Authors: Bathula Hari Babu , Oh Jinho , Jo Yeongin , Suh Young-Woong
Published: August 2019

A pair of 2-[(n-methylcyclohexyl)methyl]piperidine (H12-MBP) and its full dehydrogenation product (H0-MBP) has recently been considered as a potential liquid organic hydrogen carrier with 6.15 wt% H2 storage capacity. In the discovery of an active and stable catalyst for H2 discharge from H12-MBP at lower temperatures, a mesoporous Pd-Al2O3 catalyst (MPdA) was synthesized by a one-pot solvent deficient precipitation (SDP). In the present work, the sensitivity and effectiveness of the SDP method are examined by varying the calcination temperature and time in the preparation of the MPdA catalyst. The characterization revealed that the final properties of the MPdA catalyst greatly rely on both the calcination temperature and time. The MPdA catalyst showed better dehydrogenation activity for calcination at 600 °C than at other temperatures, because of Pd particles of smaller size with higher dispersion. Although the MPdA catalysts calcined at 600 °C for different periods of time have similar size and dispersion of Pd particles, the dehydrogenation efficiency was superior as the calcination time became shorter (e.g., 1 h), which originated from the better arrangement of Pd particles over a higher surface area. These MPdA catalysts, irrespective of the calcination time, displayed a remarkable stability in the dehydrogenation of H12-MBP owing to the protection of Pd particles by the Al2O3 layer.
Transforming Growth Factor-Beta (TGFβ) Signaling Pathway in Cholangiocarcinoma
Authors: Papoutsoglou Panagiotis , Louis Corentin , Coulouarn Cédric
Published: August 2019

Cholangiocarcinoma is a deadly cancer worldwide, associated with a poor prognosis and limited therapeutic options. Although cholangiocarcinoma accounts for less than 15% of liver primary cancer, its silent nature restricts early diagnosis and prevents efficient treatment. Therefore, it is of clinical relevance to better understand the molecular basis of cholangiocarcinoma, including the signaling pathways that contribute to tumor onset and progression. In this review, we discuss the genetic, molecular, and environmental factors that promote cholangiocarcinoma, emphasizing the role of the transforming growth factor β (TGFβ) signaling pathway in the progression of this cancer. We provide an overview of the physiological functions of TGFβ signaling in preserving liver homeostasis and describe how advanced cholangiocarcinoma benefits from the tumor-promoting effects of TGFβ. Moreover, we report the importance of noncoding RNAs as effector molecules downstream of TGFβ during cholangiocarcinoma progression, and conclude by highlighting the need for identifying novel and clinically relevant biomarkers for a better management of patients with cholangiocarcinoma.
Mutant Huntingtin Affects Diabetes and Alzheimer’s Markers in Human and Cell Models of Huntington’s Disease
Authors: Chaves Gepoliano , Stanley John , Pourmand Nader
Published: August 2019

A higher incidence of diabetes was observed among family members of individuals affected by Huntington’s Disease with no follow-up studies investigating the genetic nature of the observation. Using a genome-wide association study (GWAS), RNA sequencing (RNA-Seq) analysis and western blotting of Rattus norvegicus and human, we were able to identify that the gene family of sortilin receptors was affected in Huntington’s Disease patients. We observed that less than 5% of SNPs were of statistical significance and that sortilins and HLA/MHC gene expression or SNPs were associated with mutant huntingtin (mHTT). These results suggest that ST14A cells derived from R. norvegicus are a reliable model of HD, since sortilins were identified through analysis of the transcriptome in these cells. These findings help highlight the genes involved in mechanisms targeted by diabetes drugs, such as glucose transporters as well as proteins controlling insulin release related to mHTT. To the best of our knowledge, this is the first GWAS using RNA-Seq data from both ST14A rat HD cell model and human Huntington’s Disease.
Signaling Pathways of Type I and Type III Interferons and Targeted Therapies in Systemic Lupus Erythematosus
Authors: Chyuan I-Tsu , Tzeng Hong-Tai , Chen Ji-Yih
Published: August 2019

Type I and type III interferons (IFNs) share several properties in common, including the induction of signaling pathways, the activation of gene transcripts, and immune responses, against viral infection. Recent advances in the understanding of the molecular basis of innate and adaptive immunity have led to the re-examination of the role of these IFNs in autoimmune diseases. To date, a variety of IFN-regulated genes, termed IFN signature genes, have been identified. The expressions of these genes significantly increase in systemic lupus erythematosus (SLE), highlighting the role of type I and type III IFNs in the pathogenesis of SLE. In this review, we first discussed the signaling pathways and the immunoregulatory roles of type I and type III IFNs. Next, we discussed the roles of these IFNs in the pathogenesis of autoimmune diseases, including SLE. In SLE, IFN-stimulated genes induced by IFN signaling contribute to a positive feedback loop of autoimmunity, resulting in perpetual autoimmune inflammation. Based on this, we discussed the use of several specific IFN blocking strategies using anti-IFN-α antibodies, anti-IFN-α receptor antibodies, and IFN-α-kinoid or downstream small molecules, which intervene in Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathways, in clinical trials for SLE patients. Hopefully, the development of novel regimens targeting IFN signaling pathways will shed light on promising future therapeutic applications for SLE patients.
ROCK Inhibition Drives Resolution of Acute Inflammation by Enhancing Neutrophil Apoptosis
Authors: Galvão Izabela , Athayde Rayssa M. , Perez Denise A. , Reis Alesandra C. , Rezende Luisa , de Oliveira Vivian Louise S. , Rezende Barbara M. , Gonçalves William A. , Sousa Lirlândia P. , Teixeira Mauro M. , Pinho Vanessa
Published: August 2019

Uncontrolled inflammation leads to tissue damage and it is central for the development of chronic inflammatory diseases and autoimmunity. An acute inflammatory response is finely regulated by the action of anti-inflammatory and pro-resolutive mediators, culminating in the resolution of inflammation and restoration of homeostasis. There are few studies investigating intracellular signaling pathways associated with the resolution of inflammation. Here, we investigate the role of Rho-associated kinase (ROCK), a serine/threonine kinase, in a model of self-resolving neutrophilic inflammatory. We show that ROCK activity, evaluated by P-MYPT-1 kinetics, was higher during the peak of lipopolysaccharide-induced neutrophil influx in the pleural cavity of mice. ROCK inhibition by treatment with Y-27632 decreased the accumulation of neutrophils in the pleural cavity and was associated with an increase in apoptotic events and efferocytosis, as evaluated by an in vivo assay. In a model of gout, treatment with Y-27632 reduced neutrophil accumulation, IL-1β levels and hypernociception in the joint. These were associated with reduced MYPT and IκBα phosphorylation levels and increased apoptosis. Finally, inhibition of ROCK activity also induced apoptosis in human neutrophils and destabilized cytoskeleton, extending the observed effects to human cells. Taken together, these data show that inhibition of the ROCK pathway might represent a potential therapeutic target for neutrophilic inflammatory diseases.
JNK1 Induces Notch1 Expression to Regulate Genes Governing Photoreceptor Production
Authors: Pan Mingyu , Hu Haiyang , Wang Rui , Zhou Yi , Zhang Lele , Wang Chen , Wang Quanyi
Published: August 2019

c-Jun N-terminal kinases (JNKs) regulate cell proliferation and differentiation via phosphorylating such transcription factors as c-Jun. The function of JNKs in retinogenesis remains to be elucidated. Here, we report that knocking out Jnk1, but not Jnk2, increased the number of photoreceptors, thus enhancing the electroretinogram (ERG) responses. Intriguingly, Notch1, a well-established negative regulator of photoreceptor genesis, was significantly attenuated in Jnk1 knockout (KO) mice compared to wild-type mice. Mechanistically, light specifically activated JNK1 to phosphorylate c-Jun, which in turn induced Notch1 transcription. The identified JNK1–c-Jun–Notch1 axis strongly inhibited photoreceptor-related transcriptional factor expression and ultimately impaired photoreceptor opsin expression. Our study uncovered an essential function of JNK1 in retinogenesis, revealing JNK1 as a potential candidate for targeting ophthalmic diseases.
Somatic Mutations of lats2 Cause Peripheral Nerve Sheath Tumors in Zebrafish
Authors: Brandt Zachary J. , North Paula N. , Link Brian A.
Published: August 2019

The cellular signaling pathways underlying peripheral nerve sheath tumor (PNST) formation are poorly understood. Hippo signaling has been recently implicated in the biology of various cancers, and is thought to function downstream of mutations in the known PNST driver, NF2. Utilizing CRISPR-Cas9 gene editing, we targeted the canonical Hippo signaling kinase Lats2. We show that, while germline deletion leads to early lethality, targeted somatic mutations of zebrafish lats2 leads to peripheral nerve sheath tumor formation. These peripheral nerve sheath tumors exhibit high levels of Hippo effectors Yap and Taz, suggesting that dysregulation of these transcriptional co-factors drives PNST formation in this model. These data indicate that somatic lats2 deletion in zebrafish can serve as a powerful experimental platform to probe the mechanisms of PNST formation and progression.
Insights into Early Recovery from Influenza Pneumonia by Spatial and Temporal Quantification of Putative Lung Regenerating Cells and by Lung Proteomics
Authors: Ong Joe Wee Jian , Tan Kai Sen , Ler Siok Ghee , Gunaratne Jayantha , Choi Hyungwon , Seet Ju Ee , Chow Vincent Tak-Kwong
Published: August 2019

During influenza pneumonia, the alveolar epithelial cells of the lungs are targeted by the influenza virus. The distal airway stem cells (DASCs) and proliferating alveolar type II (AT2) cells are reported to be putative lung repair cells. However, their relative spatial and temporal distribution is still unknown during influenza-induced acute lung injury. Here, we investigated the distribution of these cells, and concurrently performed global proteomic analysis of the infected lungs to elucidate and link the cellular and molecular events during influenza pneumonia recovery. BALB/c mice were infected with a sub-lethal dose of influenza H1N1 virus. From 5 to 25 days post-infection (dpi), mouse lungs were subjected to histopathologic and immunofluorescence analysis to probe for global distribution of lung repair cells (using P63 and KRT5 markers for DASCs; SPC and PCNA markers for AT2 cells). At 7 and 15 dpi, infected mouse lungs were also subjected to protein mass spectrometry for relative protein quantification. DASCs appeared only in the damaged area of the lung from 7 dpi onwards, reaching a peak at 21 dpi, and persisted until 25 dpi. However, no differentiation of DASCs to AT2 cells was observed by 25 dpi. In contrast, AT2 cells began proliferating from 7 dpi to replenish their population, especially within the boundary area between damaged and undamaged areas of the infected lungs. Mass spectrometry and gene ontology analysis revealed prominent innate immune responses at 7 dpi, which shifted towards adaptive immune responses by 15 dpi. Hence, proliferating AT2 cells but not DASCs contribute to AT2 cell regeneration following transition from innate to adaptive immune responses during the early phase of recovery from influenza pneumonia up to 25 dpi.
Physiology, Pathology and Regeneration of Salivary Glands
Authors: Porcheri Cristina , Mitsiadis Thimios
Published: August 2019

Salivary glands are essential structures in the oral cavity. A variety of diseases, such as cancer, autoimmune diseases, infections and physical traumas, can alter the functionality of these glands, greatly impacting the quality of life of patients. To date, no definitive therapeutic approach can compensate the impairment of salivary glands, and treatment are purely symptomatic. Understanding the cellular and molecular control of salivary glands function is, therefore, highly relevant for therapeutic purposes. In this review, we provide a starting platform for future studies in basic biology and clinical research, reporting classical ideas on salivary gland physiology and recently developed technology to guide regeneration, reconstruction and substitution of the functional organs.
Parental Educational Attainment and Chronic Medical Conditions among American Youth; Minorities’ Diminished Returns
Authors: Assari Shervin , Bazargan Mohsen , Caldwell Cleopatra H.
Published: August 2019

Background: Parental educational attainment is protective against chronic medical conditions (CMCs). According to the minorities’ diminished returns (MDRs) theory, however, the health effects of socioeconomic status (SES) indicators are smaller for socially marginalized groups such as racial and ethnic minorities rather than Whites. Aims: To explore racial and ethnic differences in the effect of parental educational attainment on CMCs in a nationally representative sample of American youth. Methods: In this cross-sectional study, we used baseline data of 10,701 12–17 years old youth in the Population Assessment of Tobacco and Health (PATH; 2013). Parental educational attainment was the independent variable. The dependent variable was the number of CMCs in youth. Age, gender, and family structure were covariates. Race and ethnicity were the focal moderators. Linear and multinomial regression were applied to analyze the data. Results: Overall, higher parental educational attainment was associated with a lower number of CMCs. Race and ethnicity, however, showed significant interactions with parental educational attainment on a number of CMCs as well as 2+ CMCs, suggesting that the effect of parenting educational attainment on CMCs is significantly smaller for Black and Hispanic than White youth. Conclusions: In the United States, race and ethnicity alter the health gains that are expected to follow parental educational attainment. While White youth who are from highly educated families are most healthy, Black and Hispanic youth from highly educated families remain at higher risk for CMCs. That means, while the most socially privileged group, Whites, gain the most health from their parental education, Blacks and Hispanics, the least privileged groups, gain the least. The result is a disproportionately high number of CMCs in middle-class Blacks and Hispanics. Economic, social, public, and health policy makers should be aware that health disparities are not all due to lower SES of the disadvantaged group but also diminished returns of SES resources for them. Youth physical health disparities due to race and ethnicity exist across all SES levels.
Ohmic Contact of Pt/Au on Hydrogen-Terminated Single Crystal Diamond
Authors: Zhang Minghui , Lin Fang , Wang Wei , Li Fengnan , Wang Yan-Feng , Abbasi Haris , Zhao Dan , Chen Genqiang , Wen Feng , Zhang Jingwen , Bu Renan , Wang Hongxing
Published: August 2019

In this study, contact properties of platinum/gold (Pt/Au) on the surface of hydrogen-terminated single crystal diamond (H-SCD) were studied with several treatment conditions. The electrodes of Pt (20 nm)/Au (100 nm) were deposited on H-SCD surface by electron beam evaporation technique. Then, the specific contact resistance (ρc) of the as-fabricated sample was measured by the circular transmission line model, which showed good ohmic properties with the value of 5.65 × 10−4 Ω·cm2. To identify the thermal stability of Pt/Au/H-SCD, the sample was annealed in hydrogen ambient from 200 to 700 °C for 20 min at each temperature. As the temperature increased, ρc demonstrated better thermal stability. In addition, the barrier height was evaluated to be −0.67 ± 0.12 eV by X-ray photoelectron spectroscopy (XPS) technique.
Numerical Simulation Using Finite-Difference Schemes with Continuous Symmetries for Processes of Gas Flow in Porous Media
Authors: Markov Pavel , Rodionov Sergey
Published: August 2019

This article presents the applications of continuous symmetry groups to the computational fluid dynamics simulation of gas flow in porous media. The family of equations for one-phase flow in porous media, such as equations of gas flow with the Klinkenberg effect, is considered. This consideration has been made in terms of difference scheme constructions with the preservation of continuous symmetries, which are presented in original parabolic differential equations. A new method of numerical solution generation using continuous symmetry groups has been developed for the equation of gas flow in porous media. Four classes of invariant difference schemes have been found by using known group classifications of parabolic differential equations with partial derivatives. Invariance of necessary conditions for stability has been shown for the difference schemes from the presented classes. Comparison with the classical approach for seeking numerical solutions for a particular case from the presented classes has shown that the calculation speed is greater by several orders than for the classical approach. Analysis of the accuracy for the presented method of numerical solution generation on the basis of continuous symmetries shows that the accuracy of generated numerical solutions depends on the accuracy of initial solutions for generations.