bluuebunny/crossref_metadata_2025 huggingface dataset:
Machine Learning Model Reveals Land Use and Climate’s Role in Amazon Wildfires: Present and Future Scenarios
Authors: de Santana Mariana Martins Medeiros , de Vasconcelos Rodrigo Nogueira , Mariano Neto Eduardo , da Franca Rocha Washington de Jesus Sant’Anna
Published: September 2024

Understanding current fire dynamics in the Amazon is vital for designing effective fire management strategies and setting a baseline for climate change projections. This study aimed to analyze recent fire probabilities and project future “fire niches” under global warming scenarios across the Legal Amazon, a scale chosen for its relevance in social and economic planning. Utilizing the maximum entropy method, this study combined a complex set of predictors with fire occurrences detected during 1985–2022. It allowed for the estimation of current fire patterns and projecting changes for the near future (2020–2040) under two contrasting socioeconomic pathways. The results showed strong model performance, with AUC values consistently above 0.85. Key predictors included “Distance to Farming” (53.4%), “Distance to Non-Vegetated Areas” (11.2%), and “Temperature Seasonality” (9.3%), revealing significant influences from human activities alongside climatic predictors. The baseline model indicated that 26.5% of the Amazon has “moderate” to “very high” fire propensity, especially in the southern and southeastern regions, notably the “Arc of Deforestation”. Future projections suggest that fire-prone areas may expand, particularly in the southern border regions and near the Amazon riverbanks. The findings underscore the importance of incorporating both ecological and human factors into fire management strategies to effectively address future risks.
Optimizing Wildfire Evacuations through Scenario-Based Simulations with Autonomous Vehicles
Authors: Ali Asad , Guo Mingwei , Ahmad Salman , Huang Ying , Lu Pan
Published: September 2024

Natural disasters like hurricanes, wildfires, and floods pose immediate hazards. Such events often necessitate prompt emergency evacuations to save lives and reduce fatalities, injuries, and property damage. This study focuses on optimizing wildfire evacuations by analyzing the influence of different transportation infrastructures and the penetration of autonomous vehicles (AVs) on a historical wildfire event. The methodology involves modeling various evacuation scenarios and incorporating different intersection traffic controls such as roundabouts and stop signs and an evacuation strategy like lane reversal with various AV penetration rates. The analysis results demonstrate that specific interventions on evacuation routes can significantly reduce travel times during evacuations. Additionally, a comparative analysis across different scenarios shows a promising improvement in travel time with a higher level of AV penetration. These findings advocate for the integration of autonomous technologies as a crucial component of future emergency response strategies, demonstrating the potential for broader applications in disaster management. Future studies can expand on these findings by examining the broader implications of integrating AVs in emergency evacuations.
Effects of Stocking Density of the River Shrimp Cryphiops caementarius on Physiological and Performance Responses in a Biofloc System
Authors: Mendez Carlos Andres , Morales María Cristina , Brokordt Katherina
Published: September 2024

This study aimed to evaluate and compare the effects of biofloc technology (BFT) and clear water (CW) on water quality physiological and productive performance of juvenile freshwater Northern River shrimp, Cryphiops caementarius under three stocking densities (100, 200, and 400 shrimp m−2). Shrimp with an initial body weight of 0.44 ± 0.07 g were stocked in 18 rectangular fiberglass tanks with a water volume 150 L for 290 days. During the experiment, water quality parameters stayed within acceptable ranges for shrimp growth. The highest survival rate was recorded in BFT treatments; however, the growth performance of shrimp in the treatments with the lowest stocking density was higher than that with the highest stocking density, regardless of whether BFT or CW was used. Transcriptional levels of heat shock protein (Hsp70) and superoxide dismutase (SOD) showed significant differences (p < 0.05) between treatments, particularly in BFT. These results indicate that an initial stocking density of 200 shrimp m−2 appears to be appropriate for shrimp juveniles cultured in a BFT system. Thus, this technology emerges as an effective tool for river shrimp farmers looking to increase their stocking densities and improve the efficiency of their production systems in arid zones.
Molecular Characterization of Gymnorhynchus isuri Robinson, 1959 (Gymnorhynchidae) Infecting the Sharptail Mola Masturus lanceolatus (Liénard, 1840) from off the Coast of Kerala, India
Authors: Sarlin Pathissery John , Occhibove Flavia , Morris Sancia , Morris Sandie , Joseph Polycarp , Santoro Mario
Published: September 2024

The cestode family Gymnorhynchidae (Trypanorhyncha) comprises three genera and six valid species that, as adults, are all intestinal parasites of large pelagic sharks. Their life cycle has not been elucidated yet, but it has been proposed that copepods serve as first, pelagic euphausiids or schooling fish as second, and larger predatory fishes as third intermediate hosts. Molidae fish have been proposed as intermediate hosts for at least two gymnorhynchid species (i.e., Molicola horridus and M. uncinatus). During a parasitological survey of fish from the coast of Kerala (India), some individuals of a gymnorhynchid species were found in a sharptail mola Masturus lanceolatus. Parasites were located on the subcapsular tissue of liver showing a serpiginous route. Based on 28S rDNA molecular and phylogenetic analysis, parasites were identified as Gymnorhynchus isuri, which resulted genetically identical to G. isuri obtained from the liver of a sun fish Mola mola in the Mediterranean Sea.
Dietary β-1,3-Glucan Promotes Growth Performance and Enhances Non-Specific Immunity by Modulating Pattern Recognition Receptors in Juvenile Oriental River Prawn (Macrobrachium nipponense)
Authors: Xu Tailei , Wang Junbao , Xu Hao , Wang Zifan , Liu Yujie , Bai Hongfeng , Zhang Yixiang , Kong Youqin , Liu Yan , Ding Zhili
Published: September 2024

As a typical pathogen-associated molecular pattern (PAMP), β-1,3-glucan can engage with pattern recognition receptors (PRRs) to initiate an immune response. In this study, we investigated the effects of dietary β-1,3-glucan on growth performance, antioxidant capacity, immune response, intestinal health, and bacterial resistance in juvenile Macrobrachium nipponense. Prawns were fed with five experimental diets containing 0%, 0.05%, 0.1%, 0.2%, and 0.4% β-1,3-glucan for eight weeks. The findings demonstrated that the inclusion of β-1,3-glucan improved weight gain and survival rate in prawns. Prawns fed with β-1,3-glucan exhibited elevated activities of hepatopancreatic ACP (acid phosphatase), AKP (alkaline phosphatase), and SOD (superoxide dismutase), while MDA (malondialdehyde) content was reduced. Expression levels of PRRs related genes including LGBP (lipopolysaccharide and β-1,3-glucan binding protein), lectin, and LBP (lipopolysaccharide-binding protein) were significantly increased in prawns fed with β-1,3-glucan. Intestinal flora analysis revealed suppression of Cyanobacteria abundance at the Phylum level and enhancement in Rhodobacter abundance at the genus level in prawns fed with a 0.2% β-1,3-glucan diet. Furthermore, prawns fed with 0.1%, 0.2%, and 0.4% β-1,3-glucan demonstrated significantly higher survival rates following Aeromonas hydrophila infection. In conclusion, β-1,3-glucan can activate PRRs to improve immune responses in M. nipponese. Within the range of β-1,3-glucan concentrations set in this experiment, it is recommended to add 0.18% of β-1,3-glucan to the diet, taking into account the positive effect of β-1,3-glucan on the survival rate of M. nipponensecu.
Fish Stock Status and Its Clues on Stocking: A Case Study of Acanthopagrus schlegelii from Zhanjiang Coastal Waters, China
Authors: Lauden Hagai Nsobi , Xu Xinwen , Lyu Shaoliang , Alfatat Alma , Lin Kun , Zhang Shuo , Chen Ning , Wang Xuefeng
Published: September 2024

Global fisheries face overfishing challenges, endangering fish stock and biodiversity. While hatchery release stocking programs aim to bolster fish populations, their effectiveness remains uncertain due to challenges in data acquisition, such as obtaining stocking details and differentiating between wild and stocked individuals. This study evaluates the stock status of black sea bream (Acanthopagrus schlegelii) released in Chinese hatchery programs since the 1980s. Samples from Zhanjiang fish markets (January 2019–October 2021) underwent analysis using the length–weight relationship (LWR), length-based spawning potential ratio (LBSPR), and length-based Bayesian (LBB) methods. The male and female LWRs were W = 0.0188L2.9725 and W = 0.016L3.0284, respectively, and the observed condition factors indicated good health. The estimates imply that the region is conducive to species survival and can sustain its stocking programs. The LBSPR (SPR = 0.21 (0.17–0.25)) and LBB (B/BMSY = 1.1 (0.718–1.34), B/B0 = 0.37 (0.253–0.473)) results suggest a rebuilding population capable of replenishing to more sustainable levels. However, F/M and net selectivity estimates indicate that fishing practices target juvenile fish, limiting the reproductive potential of A. schlegelii. These findings underline the need for enhanced management strategies, including delayed harvesting and the use of size-specific fishing gear, to ensure the long-term sustainability of the species.
Droplet Contact Line Dynamics after Impact on Solid Surface: Future Perspectives in Healthcare and Medicine
Authors: Mohammad Karim Alireza
Published: September 2024

The physics of the moving contact line of an impacting droplet is widely applied in a variety of domains in rapidly advancing healthcare technology and medicine. The behavior of the dynamic contact line after impact of a biologically active droplet on a complex material surface involves complicated solid–liquid and liquid–gas interfacial interactions. Therefore, a deep understanding of such complex droplet contact line dynamics by applying the current physical models and state-of-the-art nanotechnology and artificial neural networks can be one of the ongoing promising interests in the field of interfacial physics. This review provides an overview of several scientific aspects of contact line dynamics of an impacting droplet and its influence on the current developed healthcare technology and medicine. Firstly, the potential applications in modern healthcare and personalized medicine are listed and discussed. Secondly, the theory of the moving contact line and the fundamental physical parameters related to the motion of impacting droplets are introduced. Afterwards, the current physical models of moving contact line dynamics are critically explained by emphasizing their limitations. Finally, current concerns and obstacles are summarized, and future perspectives and research directions are outlined to address poorly understood and conflicting issues.
Predicting Power Consumption Using Deep Learning with Stationary Wavelet
Authors: Frikha Majdi , Taouil Khaled , Fakhfakh Ahmed , Derbel Faouzi
Published: September 2024

Power consumption in the home has grown in recent years as a consequence of the use of varied residential applications. On the other hand, many families are beginning to use renewable energy, such as energy production, energy storage devices, and electric vehicles. As a result, estimating household power demand is necessary for energy consumption monitoring and planning. Power consumption forecasting is a challenging time series prediction topic. Furthermore, conventional forecasting approaches make it difficult to anticipate electric power consumption since it comprises irregular trend components, such as regular seasonal fluctuations. To address this issue, algorithms combining stationary wavelet transform (SWT) with deep learning models have been proposed. The denoised series is fitted with various benchmark models, including Long Short-Term Memory (LSTM), Gated Recurrent Units (GRUs), Bidirectional Gated Recurrent Units (Bi-GRUs), Bidirectional Long Short-Term Memory (Bi-LSTM), and Bidirectional Gated Recurrent Units Long Short-Term Memory (Bi-GRU LSTM) models. The performance of the SWT approach is evaluated using power consumption data at three different time intervals (1 min, 15 min, and 1 h). The performance of these models is evaluated using metrics such as Root Mean Square Error (RMSE), Mean Absolute Percentage Error (MAPE), and Mean Absolute Error (MAE). The SWT/GRU model, utilizing the bior2.4 filter at level 1, has emerged as a highly reliable option for precise power consumption forecasting across various time intervals. It is observed that the bior2.4/GRU model has enhanced accuracy by over 60% compared to the deep learning model alone across all accuracy measures. The findings clearly highlight the success of the SWT denoising technique with the bior2.4 filter in improving the power consumption prediction accuracy.
Creation of an Experimental Animal Model for the Study of Postmortem Dark Scleral Spots
Authors: Nioi Matteo , Napoli Pietro Emanuele , Nieddu Domenico , Chighine Alberto , Fossarello Maurizio , d’Aloja Ernesto
Published: September 2024

Postmortem dark scleral spots, first described by Sommer in 1833, are well-known in forensic pathology. Background/Objectives: Despite this, their presence is currently considered a nonspecific sign, and their pathogenesis has received little attention in forensic literature. In recent years, however, preliminary studies have suggested new mechanisms in their pathogenesis and links to specific types of death. This study aims to create an experimental model for studying scleral spots. Methods: Twenty sheep, already slaughtered for food, were used. After decapitation, the heads were transported to a room with known temperature and humidity. The right eye underwent eyelid excision, while the left eye’s eyelids were sutured. Continuous observation for approximately 24 h was conducted, with brief interruptions to observe the closed eyes. Results: Dark scleral spots appeared in all open eyes (20/20) after an average of 240.96 ± 58.36 min. The spots did not appear in any of the closed eyes. Conclusions: Experimental data indicate that despite the different location compared to human cadavers, this model serves as an excellent experimental framework for studying postmortem scleral spots.
Therapeutic Needs of Older Adults with Inflammatory Bowel Disease (IBD): A Systematic Review
Authors: Davis Suja P. , McInerney Rachel , Fisher Stephanie , Davis Bethany Lynn
Published: September 2024

Background/Objective: Inflammatory bowel disease (IBD) diagnosis in the elderly falls under two categories: those diagnosed at a younger age and transitioning to the elderly group (>60 years) and those diagnosed at ≥60 years of age. Although it is difficult to calculate the incidence of IBD among elderly adults precisely, it is estimated that around 10–15% of IBD in the US are diagnosed after 60 years, and approximately 13% of IBD cases are diagnosed after the age of 65 globally. The objective of this systematic review is to assess the therapeutic needs of elderly adults with IBD, focusing on quality of life (QOL), symptom presentation, mental health management, IBD medication utilization patterns, surgical outcomes, and healthcare utilization to identify gaps in IBD management. Methods: We identified 42 published articles through a database search using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses from October 2023 to June 2024. We conducted the quality appraisal of the selected studies using the Joanna Briggs Institute (JBI) critical appraisal tools. Results: Our findings indicate decreased health-related QOL, more colonic and less penetrating disease in elderly Crohn’s disease patients, and comparable symptoms between elderly and younger ulcerative colitis patients. Despite an increased trend in prescribing biologics, the elderly show decreased response rates and poor remission. Higher healthcare utilization is noted among elderly IBD patients, alongside insufficient attention to their mental health concerns. Conclusions: The findings from this systematic review offer a comprehensive synthesis of the management of elderly adults with IBD and highlight several unmet needs that warrant attention in future research and clinical practice considerations.
Resection of the Inferior Vena Cava Due to Tumor Involvement Allows Long-Term Survival in Different Neoplasms
Authors: Jaén-Torrejimeno Isabel , López-Guerra Diego , Rojas-Holguín Adela , Gómez-Martin Antonio Enrique , Blanco-Fernández Gerardo
Published: September 2024

Background: The involvement of the inferior vena cava in advanced abdominal tumors is a surgical challenge, given the high postoperative morbidity and poor long-term prognosis. Our goal was to analyze our experience, perioperative management, and results. Methods: We retrospectively evaluated short and long-term results of surgical resections of tumors with associated inferior vena cava resection performed in our facilities between 2012 and 2023. Results: A total of 25 patients were selected for our retrospective study: 3 with renal carcinoma, 5 with sarcoma, 12 with metastatic lesions, 2 with adrenal tumors, 1 pancreatic tumor, 1 extra-adrenal paraganglioma and 1 with hepatocellular carcinoma. Postoperative severe complications (Dindo-Clavien score ≥ IIIa) affected 36% of patients and the mortality rate was 8%. Partial resection with primary repair was performed in 13 patients (52%), with patch reconstruction in 6 (24%), and vascular reconstruction with prosthesis in 6 patients (24%). The mean disease-free survival was 7 months (IQR 2.5–11.5). Graft thrombosis occurred in 2 patients during follow-up. Conclusions: In selected patients, the resection of the inferior vena cava is a complex surgery with an assumable morbimortality and relative survival improvement.
Breaking Queer Silences, Building Queer Archives, and Claiming Queer Indigenous P’urhépecha Methodologies
Authors: Gómez Zamora Mario A.
Published: September 2024

In this essay, I recover queer Indigenous P’urhépecha histories in Michoacán, México, by claiming queer P’urhépecha research methods. To do so, I introduce the Indigenous methodology of talking-while-walking, which refers to how I learned to connect with P’urhépecha knowledge and traditions through the voice of my P’urhépecha grandfather. Since the colonial system eradicated queer histories from my land, I seek historical narratives about queer people in Michoacán from any source available to me, including oral histories, archives, information in the media, and interviews. I argue that queer P’urhépecha histories are unstable and non-linear, and that P’urhépecha bodies have been hunted and their histories distorted, provoking fear and false speculations about queerness among the collective. I also examine the attachment of P’urhépecha people to gender binary traditions and heteronormativity and how the narratives behind these practices relate to colonial violence and the persecution of queer P’urhépechas. Thus, I demonstrate how P’urhépecha queerness has been marginalized and simultaneously displaced from the archival records while I claim queer P’urhépecha histories and build queer P’urhépecha archives. Finally, I propose a sensitive and personal approach to queer histories guided by the voices of my queer P’urhépecha interlocutors and the histories that my P’urhépecha abuelo passed to me.
Genome-Wide Association Study Reveals the Genetic Architecture of Growth and Meat Production Traits in a Chicken F2 Resource Population
Authors: Volkova Natalia A. , Romanov Michael N. , Vetokh Anastasia N. , Larionova Polina V. , Volkova Ludmila A. , Abdelmanova Alexandra S. , Sermyagin Alexander A. , Griffin Darren K. , Zinovieva Natalia A.
Published: September 2024

Background/Objectives: For genomic selection to enhance the efficiency of broiler production, finding SNPs and candidate genes that define the manifestation of main selected traits is essential. We conducted a genome-wide association study (GWAS) for growth and meat productivity traits of roosters from a chicken F2 resource population (n = 152). Methods: The population was obtained by crossing two breeds with contrasting phenotypes for performance indicators, i.e., Russian White (slow-growing) and Cornish White (fast-growing). The birds were genotyped using the Illumina Chicken 60K SNP iSelect BeadChip. After LD filtering of the data, 54,188 SNPs were employed for the GWAS analysis that allowed us to reveal significant specific associations for phenotypic traits of interest and economic importance. Results: At the threshold value of p < 9.2 × 10−7, 83 SNPs associated with body weight at the age of 28, 42, and 63 days were identified, as well as 171 SNPs associated with meat qualities (average daily gain, slaughter yield, and dressed carcass weight and its components). Moreover, 34 SNPs were associated with a group of three or more traits, including 15 SNPs significant for a group of growth traits and 5 SNPs for a group of meat productivity indicators. Relevant to these detected SNPs, nine prioritized candidate genes associated with the studied traits were revealed, including WNT2, DEPTOR, PPA2, UNC80, DDX51, PAPPA, SSC4D, PTPRU, and TLK2. Conclusions: The found SNPs and candidate genes can serve as genetic markers for growth and meat performance characteristics in chicken breeding in order to achieve genetic improvement in broiler production.
Peculiar k-mer Spectra Are Correlated with 3D Contact Frequencies and Breakpoint Regions in the Human Genome
Authors: Hikmat Wisam Mohammed , Sievers Aaron , Hausmann Michael , Hildenbrand Georg
Published: September 2024

Background: It is widely accepted that the 3D chromatin organization in human cell nuclei is not random and recent investigations point towards an interactive relation of epigenetic functioning and chromatin (re-)organization. Although chromatin organization seems to be the result of self-organization of the entirety of all molecules available in the cell nucleus, a general question remains open as to what extent chromatin organization might additionally be predetermined by the DNA sequence and, if so, if there are characteristic differences that distinguish typical regions involved in dysfunction-related aberrations from normal ones, since typical DNA breakpoint regions involved in disease-related chromosome aberrations are not randomly distributed along the DNA sequence. Methods: Highly conserved k-mer patterns in intronic and intergenic regions have been reported in eukaryotic genomes. In this article, we search and analyze regions deviating from average spectra (ReDFAS) of k-mer word frequencies in the human genome. This includes all assembled regions, e.g., telomeric, centromeric, genic as well as intergenic regions. Results: A positive correlation between k-mer spectra and 3D contact frequencies, obtained exemplarily from given Hi-C datasets, has been found indicating a relation of ReDFAS to chromatin organization and interactions. We also searched and found correlations of known functional annotations, e.g., genes correlating with ReDFAS. Selected regions known to contain typical breakpoints on chromosomes 9 and 5 that are involved in cancer-related chromosomal aberrations appear to be enriched in ReDFAS. Since transposable elements like ALUs are often assigned as major players in 3D genome organization, we also studied their impact on our examples but could not find a correlation between ALU regions and breakpoints comparable to ReDFAS. Conclusions: Our findings might show that ReDFAS are associated with instable regions of the genome and regions with many chromatin contacts which is in line with current research indicating that chromatin loop anchor points lead to genomic instability.
Cloning and Expression of Pigeon-Derived Escherichia coli Type 1 Pilus Clusters and Analysis of Amino Acid Sequence Characteristics of Functional Proteins
Authors: Chen Junhong , Dai Wei , Wang Hang , Lei Weiqiang , Fang Guangyuan , Dai Dingzhen
Published: September 2024

Background: Type 1 pili, as an important virulence factor of E. coli, has certain homology between APEC and UPEC, but the homology degree is not clear enough. Objectives: This study aims to compare the homology between them. Methods: The recombinant bacteria were constructed by homologous recombination. The pili were observed by TEM, and the hemagglutination characteristics were determined by MHSA. The complete gene sequence was determined by sequencing, and the amino acid sequences of the functional proteins of type 1 pili of APEC and UPEC were compared. Results: TEM showed that they could express pili, which were slender, straight, and dense. Stable-pUC-fimBH has MHSA but stable-pUC-fimBG does not. The amino acid sequence similarity of FimB of NJ05 and UPEC was 98.8%, FimE was 99.4%, and the similarity between them was 51.5%. Compared with UPEC’s type 1 pili FimC and FimD sequences, the similarity was 99.52% and 87.8%, respectively. The amino acid sequence of FimA of NJ05 was 89–96%, similar to UPEC, and the N-terminal and C-terminal amino acid sequences were exactly the same. The gene sequence and amino acid sequence similarity of FimH between them were both above 99%. The similarity of the pilus binding domain of FimH was 52.8%, but only 27.6% in the receptor binding domain. A few of the same amino acid residues were found in the corresponding regions of FimA, FimF, FimG, and FimH. Conclusions: The type 1 pili of APEC and UPEC come from the same origin, which is helpful to further reveal the pathogenic mechanism of E. coli infection in the poultry respiratory tract.
Freshwater Slugs in the Caribbean: Rediscovery of Tantulidae (Acochlidimorpha, Panpulmonata) with the Description of Potamohedyle espinosai n. gen. n. sp. from Cuba
Authors: Neusser Timea P. , Onay Anabel , Pirchtner Mona , Jörger Katharina M. , Diez Yander L.
Published: September 2024

Freshwater slugs are scarce and belong exclusively to panpulmonate Acochlidimorpha. There is a radiation of eight species of large-sized slugs living benthically in rivers on tropical Indo-Pacific Islands. In the Western Atlantic, only one small interstitial slug, Tantulum elegans Rankin, 1979, is known from the Caribbean island of St. Vincent. We recently discovered a novel species of freshwater slugs in Cuba. Here, we describe Potamohedyle espinosai n. gen. n. sp., which is the first freshwater slug in the region of the Western Atlantic with a benthic lifestyle, in 3D-microanatomical and histological detail using light and scanning electron microscopy. It shows a mix of characters from different freshwater acochlidimorph genera, such as a medium body size, the presence of an osphradial ganglion, a distal gonoduct with a muscular sphincter, a penis with a solid thorn and cuticular comb, and a basal finger with a hollow stylet. Morphological adaptations to a life in freshwater include multiplicated renopericardioducts. The taxonomic character mix justifies the establishment of a novel genus within the herein diagnostically modified freshwater family Tantulidae. A molecular phylogenetic hypothesis of riverine slugs including the first Caribbean representatives suggests that the transition to freshwater occurred once along the stemline of limnic Acochlidiidae, secondarily marine Pseudunelidae and limnic Tantulidae.
Liquid Organic Hydrogen Carriers: Hydrogenation Thermodynamics of Aromatic Esters
Authors: Verevkin Sergey P. , Samarov Artemiy A. , Vostrikov Sergey V. , Rakhmanin Oleg S.
Published: September 2024

Aromatic esters such as phenyl acetates are of interest as promising liquid organic hydrogen carriers (LOHCs) due to the presence of double bonds. However, the key factor for the development of green hydrogen fuel is the production of LOHCs from renewable sources. Since the synthesis and isolation of such esters is a complex task, understanding the relationship between the chemical structures of aromatic esters and their thermodynamic properties is of great importance for their further practical use as LOHCs. Obtaining reliable thermodynamic and thermochemical properties of phenyl and benzyl phenyl acetates formed the basis of this work. Vapour pressures, enthalpies of vaporisation, and enthalpies of formation were systematically studied. An approach based on the structure–property correlation was used to confirm these quantities. Additionally, the high-level quantum-chemical method G4 was used to estimate the enthalpy of formation in the gas phase. The final stage was the assessment of the energetics of chemical reactions based on aromatic esters and their partially and fully hydrogenated analogues.
Intelligent Infrastructure and Construction: A New Transdisciplinary Journal Focusing on the Use of Information Technologies in Civil Engineering
Authors: Zheng Junxing
Published: September 2024

The field of infrastructure and construction engineering is evolving to address economic, social, and environmental resilience, facing numerous challenges along the way [...]
Co-Design Model of Support for Child and Family Health Nurse Practice with Culturally and Linguistically Diverse Families
Authors: Bonakdar Tehrani Mehrnoush , Blythe Stacy , Trajkovski Suza , Kemp Lynn
Published: September 2024

Culturally and linguistically diverse (CALD) mothers with young children face multiple inequities in accessing primary health services, such as language barriers, social isolation, low health literacy, and the availability of appropriate interpretation services. These inequities are persistent and indicate that child and family health nurse (CFHN) services, the providers of primary healthcare in many developed countries, require better support to address the needs of these families. This study engaged with CFHNs and healthcare interpreters to co-design a model of support for practice using workshops that included individual and collective brainstorming and visual representations. Transcripts of the discussion were analysed using thematic analysis. CFHNs and interpreters were able to articulate their perfect service model: a central multidisciplinary team of CFHNs, interpreters, and bilingual educators who could facilitate nurse–interpreter and nurse–interpreter–client relationships, allowing CFHNs and interpreters to do their jobs properly. This central structural component would support and be supported by rapport, trust, client choice and access, continuity of care, and cultural comfort. The study concluded that CALD mothers’ access and engagement require CFHNs to have support for their cultural comfort through the mechanism of bilingual educators and the expansion of healthcare interpreters’ role and scope in working with CFHNs in the delivery of services.
Appraising eHealth Investment for Africa: Scoping Review and Development of a Framework
Authors: Broomhead Sean C. , Mars Maurice , Scott Richard E.
Published: September 2024

Background: As opportunities grow for resource-constrained countries to use eHealth (digital health) to strengthen health systems, a dilemma arises. Wise eHealth investments require adequate appraisal to address opportunity costs. Economic appraisal techniques conventionally utilised for this purpose require sufficient economic expertise and adequate data that are frequently in short supply in low- and middle-income countries. This paper aims to identify, and, if required, develop, a suitable framework for performing eHealth investment appraisals in settings of limited economic expertise and data. Methods: Four progressive steps were followed: (1) identify required framework attributes from published checklists; (2) select, review, and chart relevant frameworks using a scoping review; (3) analyse the frameworks using deductive and inductive iterations; and, if necessary, (4) develop a new framework using findings from the first three steps. Results: Twenty-four candidate investment appraisal attributes were identified and seven relevant frameworks were selected for review. Analysis of these frameworks led to the refinement of the candidate attributes to 23 final attributes, and each framework was compared against them. No individual framework adequately addressed sufficient attributes. A new framework was developed that addressed all 23 final attributes. Conclusions: A new evidence-based investment appraisal framework has been developed that provides a practical, business case focus for use in resource-constrained African settings.
Treatment Outcomes of Tuberculosis Among Artisanal and Small-Scale Miners in Zimbabwe: A Follow-Up Observational Study Using Secondary Data
Authors: Moyo Dingani , Kavenga Fungai , Ncube Ronald Thulani , Moyo Florence , Chiboyiwa Nathan , Nyambo Andrew , Madziva Godknows , Ncube Mpokiseng , Muzvidziwa Orippa , Mperi Tafadzwa , Chigaraza Blessings , Chizana Victoria Varaidzo , Chinove Plassey Ropafadzo , Mudzingwa Frank , Mutungamiri Kudzaishe , Timire Collins
Published: September 2024

In Zimbabwe, artisanal and small-scale miners (ASMs) are a key vulnerable group with high risk for tuberculosis (TB), HIV, and silicosis. The main purpose of this study was to investigate treatment outcomes of TB among ASMs. We conducted a follow-up observational study using secondary data. We analyzed data from 208 ASMs treated for TB at two occupational health clinics. We found a high treatment success rate of 87%, comparable to the national average for drug-sensitive TB. Unsuccessful outcomes were due to death (5%) and loss to follow-up (7%). Over a quarter of ASMs had unknown HIV status. Our study is the first to document treatment outcomes of TB among ASMs in Zimbabwe. Encouragingly, this study demonstrates the possibility of achieving good TB treatment outcomes even among highly mobile populations like ASMs. Further research is needed to analyze leakages across the whole TB patient pathway among ASMs. Additionally, addressing the high rate of unknown HIV statuses among ASMs is crucial to further improve overall TB treatment outcomes in this population.
The Resurgence of Treponema pallidum Infections and Reinfections during the COVID-19 Pandemic in Greece
Authors: Tsantes Andreas G. , Toumasis Panagiotis , Domouchtsidou Aglaia , Nicolaidou Electra , Bonovas Stefanos , Stratigos Alexander , Tsakris Athanasios , Vrioni Georgia
Published: September 2024

The beginning of the COVID-19 pandemic was marked by a sharp decline in syphilis infections in many countries worldwide, including Greece. However, a resurgence of positive cases started to appear in the second half of 2020. The aim of this study was to explore the impact of the pandemic on the incidence of new syphilis infections and reinfections and analyze the sociodemographic characteristics associated with recurrent episodes. We analyzed medical records from a 14-month period after the beginning of the pandemic and compared them with the respective period before the start of the pandemic. Our participants consisted mainly of men, with a median age of 43 years, homosexual orientation, and higher education. During COVID-19, more HIV patients presented for syphilis testing (38.0% vs. 34.6%, p = 0.025). Overall, we observed almost a two-fold increase in positive syphilis cases during COVID-19 (21.1% vs. 12.4%, p < 0.001), with new infections rising from 8.4% to 13.2% and reinfections from 4.0% to 7.9%. Multivariable logistic regression analysis revealed that the COVID-19 pandemic, among different parameters (such as age, gender, sexual orientation, HIV status, and educational level), was the only factor associated with higher positive syphilis rates (OR 1.47, 95% CI: 1.07–2.01, p = 0.003). Our results highlight the need to ensure enhanced prevention and undisrupted healthcare services, with a focus on future pandemics.
Adaptation of the Nursing Activities Score in Latvia
Authors: Cerela-Boltunova Olga , Millere Inga , Trups-Kalne Ingrida
Published: September 2024

This study focuses on the adaptation and validation of the Nursing Activities Score (NAS) for use in Latvian intensive care units (ICUs) to measure nursing workload. The NAS, widely used internationally, was selected for its comprehensive ability to reflect 81% of ICU nursing activities, making it a suitable tool for assessing nursing workload in the Latvian healthcare context. The study followed a two-phase methodology: (1) expert validation using the Content Validity Index (CVI) and (2) a pilot study to assess the psychometric properties of the adapted tool. In the first phase, 10 ICU nursing experts assessed the translated NAS items, resulting in revisions to three specific paragraphs (4a, 14, and 20) based on low CVI scores. After refinement, CVIs improved from 0.6 to 0.8 for paragraphs 4a and 14, and from 0.5 to 0.9 for paragraph 20. The final CVI for all items reached 0.909. In the second phase, a pilot study was conducted in a Latvian ICU with 42 patients and 226 NAS assessments. The psychometric evaluation showed strong reliability and validity, confirming the NAS’s suitability for measuring nursing workload in this context. Cronbach’s alpha for the scale was 0.973. The adapted NAS provides a standardised method for workload assessment in Latvian ICUs, offering potential improvements in nurse resource management and patient care.
MicroRNA-195-5p Inhibits Intracerebral Hemorrhage-Induced Inflammatory Response and Neuron Cell Apoptosis
Authors: Tsai Yi-Cheng , Chang Chih-Hui , Chong Yoon Bin , Wu Chieh-Hsin , Tsai Hung-Pei , Cheng Tian-Lu , Lin Chih-Lung
Published: September 2024

Intracerebral hemorrhage (ICH) is a severe condition characterized by bleeding within brain tissue. Primary brain injury in ICH results from a mechanical insult caused by blood accumulation, whereas secondary injury involves inflammation, oxidative stress, and disruption of brain physiology. miR-195-5p may participate in ICH pathology by regulating cell proliferation, oxidative stress, and inflammation. Therefore, we assessed the performance of miR-195-5p in alleviating ICH-induced secondary brain injury. ICH was established in male Sprague–Dawley rats (7 weeks old, 200–250 g) via the stereotaxic intrastriatal injection of type IV bacterial collagenase, after which miR-195-5p was administered intravenously. Neurological function was assessed using corner turn and forelimb grip strength tests. Protein expression was assessed by western blotting and ELISA. The miR-195-5p treatment significantly improved neurological function; modulated macrophage polarization by promoting anti-inflammatory marker (CD206 and Arg1) production and inhibiting pro-inflammatory marker (CD68 and iNOS) production; enhanced Akt signalling, reduced oxidative stress by increasing Sirt1 and Nrf2 levels, and attenuated inflammation by decreasing NF-κB activation; inhibited apoptosis via increased Bcl-2 and decreased cleaved caspase-3 levels; and regulated synaptic plasticity by modulating NMDAR2A, NMDAR2B, BDNF, and TrkB expression and ERK and CREB phosphorylation. In conclusion, miR-195-5p exerts neuroprotective effects in ICH by reducing inflammation and oxidative stress, inhibiting apoptosis, and restoring synaptic plasticity, ultimately restoring behavioral recovery, and represents a promising therapeutic agent that warrants clinical studies.
Drosophila melanogaster as an Alternative Model to Higher Organisms for In Vivo Lung Research
Authors: Ehrhardt Birte , Roeder Thomas , Krauss-Etschmann Susanne
Published: September 2024

COPD and asthma are lung diseases that cause considerable burden to more than 800 million people worldwide. As both lung diseases are so far incurable, it is mandatory to understand the mechanisms underlying disease development and progression for developing novel therapeutic approaches. Exposures to environmental cues such as cigarette smoke in earliest life are known to increase disease risks in the individual’s own future. To explore the pathomechanisms leading to later airway disease, mammalian models are instrumental. However, such in vivo experiments are time-consuming and burdensome for the animals, which applies in particular to transgenerational studies. Along this line, the fruit fly Drosophila melanogaster comes with several advantages for research in this field. The short lifespan facilitates transgenerational studies. A high number of evolutionary conserved signaling pathways, together with a large toolbox for tissue-specific gene modification, has the potential to identify novel target genes involved in disease development. A well-defined airway microbiome could help to untangle interactions between disease development and microbiome composition. In the following article, Drosophila melanogaster is therefore presented and discussed as an alternative in vivo model to investigate airway diseases that can complement and/or replace models in higher organisms.
Harnessing B7-H6 for Anticancer Immunotherapy: Expression, Pathways, and Therapeutic Strategies
Authors: Lee Sunyoung , Kim Ji Hyun , Jang In-Hwan , Jo Seona , Lee Soo Yun , Oh Se-Chan , Kim Seok-Min , Kong Lingzu , Ko Jesang , Kim Tae-Don
Published: September 2024

Cancer therapies have evolved from traditional chemotherapy to more precise molecular-targeted immunotherapies, which have been associated with improved side effects and outcomes. These modern strategies rely on cancer-specific biomarkers that differentiate malignant from normal cells. The B7 family of immune checkpoint molecules is crucial for cancer immune evasion and a prime therapeutic target. B7-H6, a recently identified member of the B7 family, has emerged as a promising therapeutic target. Unlike other B7 proteins, B7-H6 is not expressed in healthy tissues but is upregulated in several cancers. It binds to NKp30, activating natural killer (NK) cells and triggering immune responses against cancer cells. This review explores the expression of B7-H6 in different cancers, the factors that regulate its expression, and its intrinsic and extrinsic pathways. Additionally, we discuss potential anticancer therapies targeting B7-H6, highlighting its significance in advancing precision medicine. Understanding the role of B7-H6 in cancer immunity may inform the development of appropriate therapies that exploit its cancer-specific expression.
The Aggravation of Neuropsychiatric Symptoms in the Offspring of a Korean Family with Intellectual Disability and Developmental Delay Caused by a Novel ARX p.Lys385Ter Variant
Authors: Han Ji Yoon , Kim Tae Yun , Gwack Jin , Park Joonhong
Published: September 2024

The ARX mutations encompass a nearly continuous spectrum of neurodevelopmental disorders (NDDs), ranging from lissencephaly to Proud syndrome, as well as infantile spasms without brain malformations, and including both syndromic and non-syndromic intellectual disabilities (IDs). We describe worsening neuropsychiatric symptoms in the offspring of a Korean family with ID/developmental delay (DD) caused by a novel ARX p.Lys385Ter variant. Sequential genetic testing was performed to investigate the ID, DD, agenesis of the corpus callosum (ACC), and developmental epileptic encephalopathy (DEE) observed in the proband. A comprehensive trio clinical exome sequencing approach using a Celemics G-Mendeliome Clinical Exome Sequencing Panel was employed. Given the clinical manifestations observed in the proband, gene panel sequencing identified a heterozygous ARX variant, c.1153A>T/p.Lys385Ter (Reference transcript ID: NM_139058.3), as the most likely cause of ID, DD, ACC, and DEE in the proband. Sanger sequencing confirmed the segregation of the ARX variant, c.1153A>T/p.Lys385Ter, with the phenotype and established the maternally inherited dominant status of the heterozygous variant in the patient, as well as in her grandmother, mother, and aunt. Our case report adds to the understanding of the female phenotype in ARX-related disorders caused by loss-of-function variants in the ARX gene. Genetic counseling for ARX families should proceed with caution, as female carriers can exhibit a wide range of phenotypes, from normal cognitive development to ID/DD, ACC, and DEE.
Untargeted Metabolomic Biomarker Discovery for the Detection of Ectopic Pregnancy
Authors: Turkoglu Onur , Citil Ayse , Katar Ceren , Mert Ismail , Quinn Robert A. , Bahado-Singh Ray O. , Graham Stewart F.
Published: September 2024

Ectopic pregnancy (EP) is the leading cause of maternal morbidity and mortality in the first trimester. Using an untargeted metabolomic approach, we sought to identify putative plasma biomarkers using tandem liquid chromatography–mass spectrometry for the detection of tubal EP. This case-control study included the prospective recruitment of 50 tubal EP cases and 50 early intrauterine pregnancy controls. To avoid over-fitting, logistic regression models were developed in a randomly selected discovery group (30 cases vs. 30 controls) and validated in the test group (20 cases vs. 20 controls). In total, 585 mass spectral features were detected, of which 221 molecular features were significantly altered in EP plasma (p < 0.05). Molecular networking and metabolite identification was employed using the Global Natural Products Social Molecular Networking (GNPS) database, which identified 97 metabolites at a high confidence level. Top significant metabolites include subclasses of sphingolipids, carnitines, glycerophosphocholines, and tryptophan metabolism. The top regression model, consisting of D-erythro-sphingosine and oleoyl-carnitine, was validated in a test group and achieved an area under receiving operating curve (AUC) (95% CI) = 0.962 (0.910–1) with a sensitivity of 100% and specificity of 95.9%. Metabolite alterations indicate alterations related to inflammation and abnormal placentation in EP. The validation of these metabolite biomarkers in the future could potentially result in improved early diagnosis.
Application of H2N-Fe3O4 Nanoparticles for Prostate Cancer Magnetic Resonance Imaging in an Animal Model
Authors: Blasiak Barbara , MacDonald David , Jasiński Krzysztof , Cheng Fong-Yu , Tomanek Boguslaw
Published: September 2024

This paper presents the efficacy of a contrast agent based on H2N-Fe3O4 nanoparticles for the detection of prostate cancer in an animal model using a preclinical 9.4 T MRI system. The relaxivities r1 and r2 of the nanoparticles were 6.31 mM−1s−1 and 8.33 mM−1s−1, respectively. Nanoparticles injected in a concentration of 2 mg Fe/mL decreased the tumor-relative T1 relaxation across all animals from 100 to 76 ± 26, 85 ± 27, 89 ± 20, and 97 ± 16 12 min 1 h, 2 h, and 24 h post injection, respectively. The corresponding T1 decrease in muscle tissues was 90 ± 20, 94 ± 23, 99 ± 12, and 99 ± 14. The relative T2 changes in the tumor were 82 ± 17, 89 ± 19, 97 ± 14, and 99 ± 8 12 min, 1 h, 2 h, and 24 h post injection, respectively, while, for muscle tissues, these values were 95 ± 11, 95 ± 8, 97 ± 6, and 95 ± 10 at the corresponding time points. The differences in the relative T1 and T2 were only significant 12 min after injection (p < 0.05), although a decrease was visible at each time point, but it was statistically insignificant (p > 0.05). The results showed the potential application of H2N-Fe3O4 nanoparticles as contrast agents for enhanced prostate cancer MRI.