bluuebunny/crossref_metadata_2025 huggingface dataset:
Highly-connected, non-redundant microRNAs functional control in breast cancer molecular subtypes
Authors: de Anda-Jáuregui Guillermo , Espinal-Enríquez Jesús , Hernández-Lemus Enrique
Published: May 2019

Transcriptional patterns are altered in breast cancer. These alterations capture the heterogeneity of breast cancer, leading to the emergence of molecular subtypes. Network biology approaches to study gene co-expression are able to capture the differences between breast cancer subtypes.

Network biology approaches may be extended to include other co-expression patterns, like those found between genes and non-coding RNA: such as mi-croRNAs (miRs). Commodore miRs are microRNAs that, based on their connectivity and redundancy in co-expression networks, have been proposed as potential control elements of biological functions.

In this work, we reconstructed miR-gene co-expression networks for each breast cancer molecular subtype. We identified Commodore miRs in three out of four molecular subtypes. We found that in each subtype, each cdre-miR had a different set of associated genes, as well as a different set of associated biological functions. We used a systematic literature validation strategy, and identified that the associated biological functions to these cdre-miRs are hallmarks of cancer.
Bacterial and fungal communities are differentially modified by melatonin in agricultural soils under abiotic stress
Authors: Madigan Andrew P. , Egidi Eleonora , Bedon Frank , Franks Ashley E. , Plummer Kim M.
Published: May 2019

An extensive body of evidence from this last decade indicates that melatonin enhances plant resistance to several biotic and abiotic stressors. This has led to an interest in the use of melatonin in agriculture to reduce negative physiological effects from environmental stresses that affect yield and crop quality. However, there are no reports regarding the effects of melatonin on soil microbial communities under abiotic stress, despite the importance of microbes for plant root health and function. Three agricultural soils associated with different land usage histories (pasture, canola or wheat) were placed under abiotic stress by cadmium (100 or 280 mg/kg) or salt (4 or 7 g/kg) and treated with melatonin (0.2 and 4 mg/kg). Automated Ribosomal Intergenic Spacer Analysis (ARISA) was used to generate Operational Taxonomic Units (OTU) for microbial community analysis in each soil. Significant differences in richness (α diversity) and community structures (β diversity) were observed between bacterial and fungal assemblages across all three soils, demonstrating the effect of melatonin on soil microbial communities under abiotic stress. The analysis also indicated that the microbial response to melatonin is governed by the type of soil and history. The effects of melatonin on soil microbes needs to be regarded in potential future agricultural applications.
The Geometric Sparse Matrix Completion Model for Predicting Drug Side effects
Authors: Galeano Diego , Paccanaro Alberto
Published: May 2019

Pair-input associations for drug-side effects are obtained through expensive placebo-controlled experiments in human clinical trials. An important challenge in computational pharmacology is to predict missing associations given a few entries in the drug-side effect matrix, as these predictions can be used to direct further clinical trials. Here we introduce the Geometric Sparse Matrix Completion (GSMC) model for predicting drug side effects. Our high-rank matrix completion model learns non-negative sparse matrices of coefficients for drugs and side effects by imposing smoothness priors that exploit a set of pharmacological side information graphs, including information about drug chemical structures, drug interactions, molecular targets, and disease indications. Our learning algorithm is based on the diagonally rescaled gradient descend principle of non-negative matrix factorization. We prove that it converges to a globally optimal solution with a first-order rate of convergence. Experiments on large-scale side effect data from human clinical trials show that our method achieves better prediction performance than six state-of-the-art methods for side effect prediction while offering biological interpretability and favouring explainable predictions.
Phosphoproteomic identification of Xin as a novel requirement for skeletal muscle disuse atrophy
Authors: Li Zhencheng , Jensen Pia , Abrigo Johanna , Henriquez-Olguin Carlos , Gingrich Molly , Rytter Nicolai , Gliemann Lasse , Richter Erik A. , Hawke Thomas , Cabello-Verrugio Claudio , Larsen Martin R. , Jensen Thomas E.
Published: May 2019

Background

Immobilization of skeletal muscle in a stretched position is associated with marked protection against disuse atrophy. Some intramyocellular changes in known proteins and post-translational modifications were previously linked to this phenomenon but there are likely many presently unknown proteins and post-translational modifications that contribute to this beneficial effect.

Methods

To identify novel proteins and phosphorylation events involved in stretch-induced reduction of disuse atrophy, we conducted a global unbiased screen of the changes occurring in skeletal muscle in control vs. 1 day and 1 week stretched cast-immobilized mouse tibialis anterior muscle, using quantitative tandem mass spectrometry on HILIC-fractionated muscle peptides with follow-up studies in transgenic mice and humans.

Results

Our mass spectrometry analyses detected 11714 phosphopeptides and 2081 proteins, of which 53 phosphopeptides and 5 proteins, 125 phosphopeptides and 43 proteins were deregulated after 1D and 7D of stretched immobilization, respectively. The sarcomere and muscle tendinous junction-associated putative multi-adaptor protein Xin was among the most highly upregulated proteins both in terms of phosphorylation and protein expression and was confirmed to increase with stretch but not disuse atrophy in mice and to increase and decrease with exercise and cast immobilization, respectively, in humans. Xin-/-mice were partially protected against disuse but not denervation atrophy in both stretched and flexed immobilized muscles compared to WT.

Conclusion

This study identified Xin as a novel protein involved in disuse atrophy and also provides a resource to guide future hypothesis-driven investigations into uncovering critical factors in the protection against disuse atrophy.
Ubiquitination of the yeast receptor Atg32 modulates mitophagy
Authors: Vigié Pierre , Gonzalez Cécile , Manon Stephen , Bhatia-Kissova Ingrid , Camougrand Nadine
Published: May 2019

Mitophagy, the process that degrades mitochondria selectively through autophagy, is involved in the quality control of these organelles. In yeast, the presence of the Atg32 protein on the outer mitochondrial membrane allows for the recognition and targeting of superfluous or damaged mitochondria for degradation. Some posttranslational modifications, such as phosphorylation, are crucial for the execution of the mitophagy process. In our study, we showed that in the stationary phase of growth, and to a lesser extent during starvation, the Atg32 protein level decreases. The fact that a decline in Atg32 level can be prevented by inhibition of the proteolytic activity of proteasome may indicate that Atg32 is also ubiquitylated. In fact, mass spectrometry analysis of purified Atg32 protein showed ubiquitination of lysine residue in position 282. These different patterns of posttranslational modifications of Atg32 could allow cells to control the mitophagy process carefully.
Aggregated and hyperstable damage-associated molecular patterns are released during ER stress to modulate immune function
Authors: Andersohn Alexander , Garcia M. Iveth , Fan Ying , Thompson Max C. , Akimzhanov Askar M. , Abdullahi Abdikarim , Jeschke Marc G. , Boehning Darren
Published: May 2019

Chronic ER stress occurs when protein misfolding in the ER lumen remains unresolved despite activation of the unfolded protein response. We have shown that traumatic injury such as a severe burn leads to chronic ER stressin vivoleading to systemic inflammation which can last for more than a year. The mechanisms linking chronic ER stress to systemic inflammatory responses is not clear. Here we show that induction of chronic ER stress leads to the release of known and novel damage-associated molecular patterns (DAMPs). The secreted DAMPs are aggregated and markedly protease resistant. ER stress-derived DAMPs activate dendritic cells which are then capable of polarizing naïve T cells. Our findings indicate that induction of chronic ER stress may lead to the release of hyperstable DAMPs into the circulation resulting in persistent systemic inflammation and adverse outcomes.
Cotranslational folding cooperativity of contiguous domains of α-spectrin
Authors: Kemp Grant , Nilsson Ola B. , Tian Pengfei , Best Robert B. , von Heijne Gunnar
Published: May 2019

Proteins synthesized in the cell can begin to fold during translation before the entire polypeptide has been produced, which may be particularly relevant to the folding of multidomain proteins. Here, we study the cotranslational folding of adjacent domains from the cytoskeletal protein α-spectrin using Force Profile Analysis (FPA). Specifically, we investigate how the cotranslational folding behavior of the R15 and R16 domains are affected by their neighboring R14 and R16, and R15 and R17 domains, respectively. Our results show that the domains impact each other’s folding in distinct ways that may be important for the efficient assembly of α-spectrin, and may reduce its dependence on chaperones. Furthermore, we directly relate the experimentally observed yield of full-length protein in the FPA assay to the force exerted by the folding protein in pN. By combining pulse-chase experiments to measure the rate at which the arrested protein is converted into full-length protein with a Bell model of force-induced rupture, we estimate that the R16 domain exerts a maximal force on the nascent chain of ∼15 pN during cotranslational folding.

Significance

In living cells, proteins are produced in a sequential way by ribosomes. This vectoral process allows the growing protein chain to start to fold before translation has been completed. Thereby, cotranslational protein folding can be significantly different than the folding of a full-length protein in isolation. Here we show how structurally similar repeat domains, normally produced as parts of a single long polypeptide, affect the cotranslational folding of their neighbors. This provides insight into how the cell may efficiently produce multidomain proteins, paving the way for future studiesin vivoor with chaperones. We also provide an estimated magnitude of the mechanical force on the nascent chain generated by cotranslational folding, calculated from biochemical measurements and molecular dynamics simulations.
The evolution of parasite host range in genetically diverse host populations
Authors: Gibson Amanda K , Baffoe-Bonnie Helena S , Penley McKenna J , Lin Julie , Owens Raythe , Khalid Arooj , Morran Levi T.
Published: May 2019

Parasites vary enormously in their host range. Why are some parasites specialists and others generalists? We tested the hypothesis that genetically diverse host populations select for parasites with broader host ranges than genetically homogeneous host populations. To do so, we selected for increased killing ability of the bacterial parasiteSerratia marcescensin populations of the hostCaenorhabditis elegansthat were either diverse (50% mix of genotypes N2 and LTM1) or homogenous (100% N2 or LTM1). We found mixed support for the hypothesis. After 20 generations of selection, parasites selected in diverse host populations maintained a broad host range, as shown by the retention of high killing ability of a novel host genotype. Parasites selected in diverse populations killed N2 hosts equally well as parasites selected in homogenous populations of N2. However, N2-selected parasites lost killing ability against the novel host, consistent with the evolution of narrow host ranges in homogenous environments. In contrast, parasites selected in homogenous LTM1 populations did not specialize: they did not increase in their killing ability on LTM1 and did not lose killing ability against the novel host. Our results argue that the evolution of host range depends upon both the identity and diversity of hosts that a parasite encounters.
Genetic editing of SEC61, SEC62, and SEC63 abrogates human cytomegalovirus US2 expression in a signal peptide-dependent manner
Authors: Schuren Anouk B.C. , Boer Ingrid G.J. , Bouma Ellen , Lebbink Robert Jan , Wiertz Emmanuel J.H.J.
Published: May 2019

Newly translated proteins enter the ER through the SEC61 complex, via either co- or post-translational translocation. In mammalian cells, few substrates of post-translational SEC62- and SEC63-dependent translocation have been described. Here, we targeted all components of the SEC61/62/63 complex by CRISPR/Cas9, creating knock-outs or mutants of the individual subunits of the complex. We show that functionality of the human cytomegalovirus protein US2, which is an unusual translocation substrate with a low-hydrophobicity signal peptide, is dependent on expression of not only SEC61α, -β, and -γ, but also SEC62 and SEC63, suggesting that US2 may be a substrate for post-translational translocation. This phenotype is specific to the US2 signal peptide.
Metabolic overlap in environmentally diverse microbial communities
Authors: Hester Eric R. , Jetten Mike S.M. , Welte Cornelia U. , Lücker Sebastian
Published: May 2019

The majority of microbial communities consist of hundreds to thousands of species, creating a massive network of organisms competing for available resources within an ecosystem. In natural microbial communities it appears that many microbial species have highly redundant metabolisms and seemingly are capable of utilizing the same substrates. This is paradoxical, as theory indicates that species requiring a common resource should outcompete one another. To better understand why microbial species can co-exist, we developed Metabolic Overlap (MO) as a new metric to survey the functional redundancy of microbial communities at the genome scale across a wide variety of ecosystems. Using metagenome-assembled genomes, we surveyed over 1200 studies across ten ecosystem types. We found the highest MO in extreme (i.e., low pH/high temperature) and aquatic environments, while the lowest MO was observed in communities associated with animal hosts, or the built/engineered environment. In addition, different metabolism subcategories were explored for their degree of metabolic overlap. For instance, overlap in nitrogen metabolism was among the lowest in Animal and Engineered ecosystems, while the most was in species from the Built environment. Together, we present a metric that utilizes whole genome information to explore overlapping niches of microbes. This provides a detailed picture of potential metabolic competition and cooperation between species present in an ecosystem, indicates the main substrate types sustaining the community and serves as a valuable tool to generate hypotheses for future research.
Sub-lethal insecticide exposure affects host biting efficiency of Kdr-resistant Anopheles gambiae
Authors: Diop Malal M , Chandre Fabrice , Rossignol Marie , Porciani Angélique , Chateau Mathieu , Moiroux Nicolas , Pennetier Cédric
Published: May 2019

Abstract

The massive use of insecticide-treated nets (ITNs) has drastically changed the environment for malaria vector mosquitoes, challenging their host-seeking behaviour and biting success. Here, we investigated the effect of a brief exposure to an ITN on the biting behaviour of Anopheles mambiae mosquitoes and the interaction between such behaviour and the kdr mutation that confers resistance to pyrethroids. To this aim, we developed a video assay to study the biting behaviour of mosquitoes with similar genetic background, but different kdr locus genotypes (SS i.e. homozygous susceptible, RS i.e. heterozygous and RR i.e. homozygous resistant), after a brief exposure to either control untreated nets or one of two types of pyrethroid-treated nets (deltamethrin or permethrin). In presence of untreated nets, the kdr mutation did not influence mosquito blood feeding success but caused differences in feeding and prediuresis durations and blood meal size. Exposure to deltamethrin ITN decreased the blood feeding success rate of RR and RS mosquitoes, whereas in presence of permethrin ITN, the kdr mutation increased the blood-feeding success of mosquitoes. Exposure to the two types of pyrethroid-treated nets reduced feeding duration, prediuresis duration and blood meal size of all three genotypes. Our study demonstrates a complex interaction between insecticide exposure and the kdr mutation on the biting behavior of mosquitoes, which may substantially impact malaria vector fitness and disease transmission.
Obesity-induced gut microbiota dysbiosis can be ameliorated by fecal microbiota transplantation: a multiomics approach
Authors: Guirro Maria , Costa Andrea , Gual-Grau Andreu , Herrero Pol , Torrell Helena , Canela Núria , Arola Lluís
Published: May 2019

Obesity and its comorbidities are currently considered an epidemic, and the involved pathophysiology is well studied. Recently, the gut microbiota has emerged as a new potential therapeutic target for the treatment of obesity. Diet and antibiotics are known to play crucial roles in changes in the microbiota ecosystem and the disruption of its balance; therefore, the manipulation of gut microbiota may represent a strategy for obesity treatment. Fecal microbiota transplantation, during which fecal microbiota from a healthy donor is transplanted to an obese subject, has aroused interest as an effective approach for the treatment of obesity. To determine its success, a multiomics approach was used that combined metagenomics and metaproteomics to study microbiota composition and function.

To do this, a study was performed in rats that evaluated the effect of a hypercaloric diet on the gut microbiota, and this was combined with antibiotic treatment to deplete the microbiota before fecal microbiota transplantation to verify its effects on gut microbiota-host homeostasis. Our results showed that a high-fat diet induces changes in microbiota biodiversity and alters its function in the host. Moreover, we found that antibiotics depleted the microbiota enough to reduce its bacterial content. Finally, we assessed the use of fecal microbiota transplantation as an obesity therapy, and we found that it reversed the effects of antibiotics and reestablished the microbiota balance, which restored normal functioning and alleviated microbiota disruption.
Diversification and collapse of a telomere elongation mechanism
Authors: Saint-Leandre Bastien , Nguyen Son C. , Levine Mia T.
Published: May 2019

In most eukaryotes, telomerase counteracts chromosome erosion by adding repetitive sequence to terminal ends. Drosophila melanogaster instead relies on specialized retrotransposons that insert exclusively at telomeres. This exchange of goods between host and mobile element—wherein the mobile element provides an essential genome service and the host provides a hospitable niche for mobile element propagation—has been called a “genomic symbiosis.” However, these telomere-specialized, jockey family retrotransposons may actually evolve to “selfishly” overreplicate in the genomes that they ostensibly serve. Under this model, we expect rapid diversification of telomere-specialized retrotransposon lineages and, possibly, the breakdown of this ostensibly symbiotic relationship. Here we report data consistent with both predictions. Searching the raw reads of the 15-Myr-old melanogaster species group, we generated de novo jockey retrotransposon consensus sequences and used phylogenetic tree-building to delineate four distinct telomere-associated lineages. Recurrent gains, losses, and replacements account for this retrotransposon lineage diversity. In Drosophila biarmipes, telomere-specialized elements have disappeared completely. De novo assembly of long reads and cytogenetics confirmed this species-specific collapse of retrotransposon-dependent telomere elongation. Instead, telomere-restricted satellite DNA and DNA transposon fragments occupy its terminal ends. We infer that D. biarmipes relies instead on a recombination-based mechanism conserved from yeast to flies to humans. Telomeric retrotransposon diversification and disappearance suggest that persistently “selfish” machinery shapes telomere elongation across Drosophila rather than completely domesticated, symbiotic mobile elements.
Assembly tolerance analysis based on the Jacobian model and skin model shapes
Authors: Liu Ting , Cao Yan-Long , Zhao Qijian , Yang Jiangxin , Cui Lujun
Published: May 2019

Purpose

The purpose of this paper is to carry out an assembly tolerance analysis by means of a combined Jacobian model and skin model shape. The former is based on small displacements modeling of points using 6 × 6 transformation matrices of open kinematic chains in robotics. The latter easily models toleranced features with all kinds of geometric deviations.

Design/methodology/approach

This paper presents the procedure of performing tolerance analysis by means of the Jacobian model and skin model shape for assemblies. The point cloud-based discrete representative is able to model the actual toleranced surfaces instead of the ideal or associated ones in an assembly, which brings the simulation tools closer to reality.

Findings

The proposed method has the advantage of skin model shape which is suitable for geometric tolerances management along the product life cycle and contact analysis of kinematic small variations, as well as, with the Jacobian, enabling transformation of locally expressed parts deviations to globally expressed functional requirements. The result of the case study shows the accuracy of the method.

Research limitations/implications

The proposed approach has not been developed fully; other functional features such as the pyramid are still ongoing challenges.

Practical implications

It is an effective method for supporting design, manufacturing and inspection by providing a quantitative analysis of the effects of multi-tolerances on the final functional key characteristics and for predicting the quality level.

Originality/value

The paper is original in taking advantages of both Jacobian model and skin model shape to consider all geometric tolerances in assembly.
Beyond gaming
Authors: Hooker Robert , Wasko Molly , Paradice David , Teigland Robin , Hofacker Charles
Published: May 2019

Purpose

The purpose of this paper is to test flow as a construct distinctly separate from its antecedents, and the resulting impact on brand attitudes and purchase intent within realistic online shopping three-dimensional virtual environments (3-DVEs).

Design/methodology/approach

This research utilizes a field study, combined with a survey research design of 348 3-DVE participants.

Findings

The findings reveal that flow is not a unidimensional construct but rather, a mental state that should be measured separately from its antecedents, with the possibility that certain antecedents may be more relevant than others in different activities. Furthermore, the authors received strong support for the overarching hypothesized model separating the nine dimensions associated with engagement in an activity as antecedents of flow, which, in turn, influences brand attitude and thereafter purchase intention.

Research limitations/implications

This study was limited to one type of activity within one 3-DVE but is balanced by the difficulty in gaining access to organizations and computer-mediated environments of this type for examining such phenomena.

Practical implications

Businesses using 3-DVEs to market brands should focus their efforts on creating highly enjoyable experiences that result in the feeling of a loss of sense of time for the participants in order to ultimately positively influence purchase intentions.

Originality/value

Prior research focused on fantasy-based, game-oriented environments, while also neglecting to test all elements of flow, examining “similar to flow” constructs or measuring flow as a unidimensional construct.
Examining the legitimacy of Sukuk structure via Shariah pronouncements
Authors: Ahmed Essia Ries , Islam Aminul , Amran Azlan Bin
Published: May 2019

Purpose

The purpose of this paper is to investigate and detect disclosure on the legitimacy identity of Sukuk structure in Shariah pronouncements. The study also examines the role of Shariah supervisory board on disclosure of legitimacy in Shariah pronouncements.

Design/methodology/approach

A total of 54 Islamic Sukuk in Malaysia representing the whole population were selected. Secondary data were used and sourced from Shariah pronouncements on Sukuk. Using descriptive statistics, the existence and the extent of existence of legitimacy disclosure on Sukuk in Islamic financial institutions in Malaysia were ascertained.

Findings

Using descriptive statistics, the existence and the extent of existence of legitimacy disclosure on Sukuk in Islamic financial institutions in Malaysia was ascertained. The research found that there is empirical evidence showing the effect of Shariah supervisory board on disclosure of legitimacy identity in Shariah pronouncements.

Originality/value

This study offers significant contribution to existing legitimacy of Sukuk structure literature. The study added a new discussion, i.e. legitimacy disclosure on the Sukuk. The study is intended to increase public awareness for legitimacy identity of Sukuk in the importance of Shariah compliancy not only in the form but also in substance. In addition, this study will give more benefits for Islamic financial institutions in the sense that they should amend some of their regulatory frameworks to push the Sukuk market investors to move toward asset-backed structure.
Currency returns during democratic transition: evidence from Tunisia
Authors: Saad Ghadi , Bouraoui Taoufik
Published: May 2019

Purpose

The purpose of this paper is to investigate the question whether democratic transition elections influence currency returns. Also, the paper examines the behavior of the currency market around these elections in Tunisia.

Design/methodology/approach

Empirical data are collected from the International Monetary Fund, the Central Bank of Tunisia and the Tunisian stock market websites. The paper employs event study analysis using a market model and investigates abnormal currency returns around the four election events that occurred during the period of democratic transition in Tunisia (2011–2015). A robustness test is also conducted to control for monetary policy effects.

Findings

The results indicate that democratic transition does impact currency returns. The authors did not find any significant effect on the events dates (t0). However, event windows around the elections days reacted significantly to the events. The authors notice a significant decrease in cumulative abnormal returns (CARs) at event periods leading up to the elections. Post-event windows perceived negative CARs in the first and second election, and positive CARs in the last two elections. The authors also find that the change in the victors of the elections does not cause major differences to CARs. Further, the authors do not find significant results when controlling for inflation and interest rate.

Originality/value

There is no evidence yet on how democratic transition elections can affect currency returns. Given that currency is a leading indicator of the performance of the financial sector, this paper should provide policymakers with new evidence on the response of currency returns to democratic transition.
CFO appointment and debt-equity choice
Authors: Mishra Suchismita , Talukdar Bakhtear , Upadhyay Arun
Published: May 2019

Purpose

There is some evidence that firms appoint internal candidates to exploit their unique firm specific knowledge and that the type of appointments may have signaling value to the market. However, these studies are limited to chief executive officer appointments whereas other top executives could also play an important role in corporate decision making. The purpose of this paper is to focus on the chief financial officer (CFO) appointments and firm’s debt-equity choice.

Design/methodology/approach

The authors employ a multiple regression framework. To control for potential endogeneity, the authors use an instrumental variable approach with both two-stage least squares and generalized method of moments estimators.

Findings

The authors find that firms with internal CFO hires issue more equity than firms that hire from the external labor market. The authors also find that internal CFOs significantly reduce information asymmetry (IA), which may lower market risk and the cost of financing through equity issues. Furthermore, consistent with the value maximizing role of reduced IA the authors find that this effect is concentrated in value firms. In firms with higher IA this preference for equity by the internal CFO may be weaker as even internal CFOs will prefer debt financing for its disciplining role and to reduce IA. A subsample analysis with growth firms shows this diminishing impact on the financing choice of an internal CFO.

Originality/value

This study provides important information about the influence the CFO has on a firm’s capital structure decisions.
Banana peel in dyeing of polyamide/elastane blend fabric
Authors: Yıldırım Leyla , Erdem Ìşmal Özlenen
Published: May 2019

Purpose

The purpose of this paper is to show the dyeing effect of banana peel on polyamide fabric by using various mordants and to reveal alternatives to metallic mordants.

Design/methodology/approach

The simultaneous mordanting method was used in the dyeing process.

Findings

From environmental and economical points of view, this paper studies the use of a natural waste product in textile dyeing. Assessment of domestic organic wastes will provide new possibilities for valorization of biomaterials. It is concluded that colorimetric data are affected by the amount of plant used in extraction, amount and type of mordant and pH of dye bath. Tin II chloride ensured the lightest color shades. No alternative mordants could be presented to iron II sulfate and tin II chloride, as they generated completely different color shades. Acids can be an alternative to 0.8 g/L alum mordant. Ammonium sulfate and sodium acetate also generated similar colors to alum. Ammonium sulfate can be substituted for citric acid and alum. Banana peel can be considered as a natural dye source for polyamide elastane blend fabric.

Originality/value

Banana peel can be suggested as a natural colorant with good wash fastness for dyeing of polyamide elastane blend fabric.
Colour management in circular economy: decolourization of cotton waste
Authors: Määttänen Marjo , Asikainen Sari , Kamppuri Taina , Ilen Elina , Niinimäki Kirsi , Tanttu Marjaana , Harlin Ali
Published: May 2019

Purpose

While aiming to create methods for fibre recycling, the question of colours in waste textiles is also in focus; whether the colour should be kept or should be removed while recycling textile fibre. More knowledge is needed for colour management in a circular economy approach.

Design/methodology/approach

The research included the use of different dye types in a cotton dyeing process, the process for decolourizing and the results. Two reactive dyes, two direct dyes and one vat dye were used in the study. Four chemical treatment sequences were used to evaluate colour removal from the dyed cotton fabrics, namely, HCE-A, HCE-P-A, HCE-Z-P-A and HCE-Y-A.

Findings

The objective was to evaluate how different chemical refining sequences remove colour from direct, reactive and vat dyed cotton fabrics, and how they influence the specific cellulose properties. Dyeing methods and the used refining sequences influence the degree of colour removal. The highest achieved final brightness of refined cotton materials were between 71 and 91 per cent ISO brightness, depending on the dyeing method used.

Research limitations/implications

Only cotton fibre and three different colour types were tested.

Practical implications

With cotton waste, it appears to be easier to remove the colour than to retain it, especially if the textile contains polyester residues, which are desired to be removed in the textile refining stage.

Originality/value

Colour management in the CE context is an important new track to study in the context of the increasing amount of textile waste used as a raw material.
Biomimetic economy: human ecological-economic systems emulating natural ecological systems
Authors: Tamayo Unai , Vargas Gustavo
Published: May 2019

Purpose

The purpose of this paper is to examine the use of biomimicry to inspire sustainable development in economic systems. The research purpose is to explore the link between ecological systems and economic systems to highlight applied environmental solutions. The goal is to propose some driver to develop sustainable business practices inspired on the principles of biomimicry.

Design/methodology/approach

This paper provides a theoretical approach that builds the basis for a better understanding of the relationship between nature and sustainable economic decisions. The premise is that in the field of sustainable development, strategies based on “learning from nature” are useful. Furthermore, the concept of biomimicry provides principles and tools specifically aimed at design practice.

Findings

The complexity of economic systems has shown that high levels of abstraction are required when conceptualising problems and explanations related with nature-inspired solutions. Stakeholder engagement and transdisciplinary collaboration are required to face long-term environmental challenges. Moreover, the exploratory analysis applied in this paper appeared suitable to compile existing literature.

Practical implications

The study provides some general guidelines and empirical approach through case studies that could help decision makers convert nature-inspired alternatives into valuable strategic business opportunities. Although presented practical cases are framed in the local sphere (i.e. the Basque Country), they can serve as references in other international contexts.

Social implications

New business models should recognize the positive synchronization between well-managed social, environmental and economic systems.

Originality/value

The proposed ideas deepen the understanding on the sustainable development and the link between ecological and economic systems. In fact, the concept of biomimetic economy has not been dealt with or developed in depth in previous academic works, nor has it been published thoroughly in the field of research.
Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models
Authors: Liang Tisong , Tao Qingfeng , Guan Rongfa , Cao Guozhou , Shen Haitao , Liu Zhenfeng , Xia Qile
Published: May 2019

The aim of this study was to obtain adequate and detailed information about the antioxidant and antiproliferative activities of C3G and C3G liposomes in different cell culture models. The Caco‐2 cells were cultured in 2D and 3D cell culture models, the H2O2 was used to construct the cell damage model and then the cells treated with C3G and C3G liposomes. The antioxidant activity and antiproliferative activities of C3G liposomes on Caco‐2 cells were investigated. We observed the morphology of cells and measured the cell viability, the activity of glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T‐AOC), and the content of malondialdehyde (MDA) in Caco‐2 cells treated with H2O2, C3G, and C3G liposomes. The results showed that the Caco‐2 cells cultured in the 3D culture model formed a 3D structure and tight spheroids and showed the increase of cell activity in 3D cell culture model, compared with the 2D cell culture model. The C3G and C3G liposomes can enhance the activities of GSH, SOD, and T‐AOC but decrease the MDA content after H2O2 treatment, while the changes were different in 2D and 3D cells culture models. This study revealed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model.

Practical Application

The results of this study showed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. Our work provides a method for evaluating antioxidant activity of C3G liposomes in different cell models and provided certain theoretical basis for the follow‐up research.
Effects of immediate versus gradual nicotine reduction in cigarettes on biomarkers of biological effects
Authors: Hatsukami Dorothy K. , Luo Xianghua , Heskin Alisa K. , Tang Mei Kuen , Carmella Steven G. , Jensen Joni , Robinson Jason D. , Vandrey Ryan , Drobes David J. , Strasser Andrew A. , al'Absi Mustafa , Leischow Scott , Cinciripini Paul M. , Koopmeiners Joseph , Ikuemonisan Joshua , Benowitz Neal L. , Donny Eric C. , Hecht Stephen S.
Published: May 2019

Aim

A previous study showed significantly greater reductions in number of cigarettes smoked and biomarkers of toxicant and carcinogen exposure in smokers assigned to immediate reduction of nicotine in cigarettes to very low levels versus gradually over time or continued smoking of normal nicotine content cigarettes. This study examines the effects of these approaches on selected biomarkers associated with harmful biological effects.

Design

Three‐arm, randomized controlled trial.

Setting

Ten United States academic institutional sites.

Participants

Daily smokers uninterested in quitting smoking with a mean age of 45.1 [standard deviation (SD) = 13.4)] years and smoking 17.1 (SD = 8.5) cigarettes/day; 43.9% (549 of 1250) female; 60.6% (758 of 1250) white ethnicity.

Interventions

(1) Smoking cigarettes where nicotine content was immediately reduced to very low levels (n = 503); (2) smoking cigarettes where nicotine content was gradually reduced, with dose changes occurring monthly (n = 498); and (3) continued smoking with normal nicotine content cigarettes (n = 249).

Measurements

Smokers were assessed at baseline while smoking their usual brand cigarettes, and again at 4, 8, 12, 16 and 20 weeks. Outcomes were areas under the concentration time curve (AUC) for the period of study of biomarkers of inflammation, oxidative stress and hematological parameters.

Findings

No consistent significant differences were observed across groups (Bayes factors showing data to be insensitive), with the only exception being red blood cell size variability, which was observed to be lower in the immediate versus gradual nicotine reduction [mean difference =  −0.11; 95% confidence interval (CI) = –0.18, −0.04, P = 0.004] and normal nicotine control groups (mean difference = − 0.15, 95% CI = –0.23, −0.06, P = 0.001).

Conclusion

It remains unclear whether switching to very low nicotine cigarettes leads to a short‐term reduction in biomarkers of tobacco‐related harm.
Using the MaxEnt model for assessing the impact of climate change on the Aurasian Aleppo pine distribution in Algeria
Authors: Garah Kenza , Bentouati Abdallah
Published: May 2019

In the context of recent climate changes and their impact on the vegetation cover in the southern shore of the Mediterranean, this paper deals with the potential distribution in the present and the future of the Aleppo pine in the north‐east of Algeria. Accordingly, this study focuses on modelling of the spatiotemporal distribution of the Aurasian Aleppo pine, by means of MaxEnt program. The models developed with MaxEnt showed good predictivity with AUC > 0.80 for the RCP 4.5 and RCP 8.5 scenarios projected, respectively, for 2055 and 2085. The results showed that the annual thermal amplitude followed by altitude appear to be the main factors of the spatiotemporal distribution of Aleppo pine in the study area. Under current conditions, only 20% of the territory seems favourable to the presence of the Aleppo pine. However, future predictions expect an extension of the areas classified as “moderately favourable” to the Aleppo pine. In response to climate changes, the Aurasian Aleppo pine may display two contrasted tendencies: a progressive evolution in the north, and a regressive evolution in the south of the Aurès region as well as a displacement of suitable areas for Aleppo pine to the north.
Dehumanization in War and Peace: Encounters with Lebanon's Ex‐Militia Fighters
Authors: Hermez Sami
Published: May 2019

Between 2006 and 2009, I attended various peacebuilding and antiwar events in Lebanon, from dialogue sessions and workshops to lectures and demonstrations. One refrain at these events was that war should be avoided because it was dehumanizing or, in a slightly different form, that it results in a loss of humanity. Alongside these gatherings, I conducted interviews and ethnographic fieldwork with former militia fighters from Lebanon's war (1975–1990). Together, these encounters led me to question notions of dehumanization in discourses of war and peace. Did war dehumanize? Did dehumanization lead to war? Did perpetrators lose their humanity through acts of killing? Did their actions stem from the dehumanization of the other? This article explores these questions and interrogates the notion of humanity. I argue that in allowing the notion of humanity to shape our politics, and in centering the loss of humanity in discourses of war and peace, both war and peace are invariably depoliticized. [war, dehumanization, combatants, humanity, Lebanon]
Obesity and hypertension contribute to prolong QRS complex duration among middle‐aged adults
Authors: Dzikowicz Dillon J. , Carey Mary G.
Published: May 2019

Background

Obesity and hypertension are comorbid diseases, which influence cardiac structure, and are associated with increased risk for cardiovascular events. The QRS duration (QRSd) reflects ventricular depolarization, and increased QRSd is associated with poor cardiovascular outcomes. QRS duration may be influenced by obesity and HTN, and reflect the increased risk factor for poor cardiovascular outcomes. The purpose of this analysis was to assess the relations between obesity, hypertension, and the interaction between them on QRSd.

Methods

In this secondary data analysis, firefighters without documented cardiovascular disease in normal sinus rhythm were included. Twelve‐lead 24‐hr ECG Holter monitors measured mean QRSd. Body mass index (BMI) and resting blood pressure (BP) were measured and categorized. Univariate linear regression models were produced using BMI, BP, and the interaction between BMI and BP as factors associated with QRSd. Multivariate models adjusting for multiple covariates were also produced.

Results

Seventy‐seven firefighters were included and most (89.4%) were overweight or obese. After covariate adjustment, BMI (p = 0.028), BMI categorization (p = 0.020), and the interaction between BMI and systolic BP (p = 0.021) were associated with prolong QRSd.

Conclusions

Increased BMI and an interaction between BMI and systolic BP were independently associated with prolongation of the QRS complex. Determination of the underlying cardiac structures responsible prolongation of the QRSd is recommended for further research.
Donkey pericardium compares favorably with commercial xenopericardia used in the manufacture of transcatheter heart valves
Authors: Mao Jifu , Rassoli Aisa , Tong Yiwei , Rouse Elizabeth Nicole , Le‐Bel Gaёtan , How Daniel , Germain Lucie , Fatouraee Nasser , Zhang Ze , Reed Robert R. , Guidoin Robert
Published: May 2019

Transcatheter aortic valve implantation (TAVI) has gained considerable acceptance in the past decade due to its lower risks than conventional open‐heart surgery. However, the deformation and delamination of the leaflets during the crimping procedure have raised questions about the durability and long‐term serviceability of the pericardium tissue from which the leaflets are made. The collagen architecture, wall thickness and mechanical properties of donkey pericardium were investigated to assess its suitability as an alternative material for the manufacture of heart valves. Coupons sampled from different locations of donkey pericardium were investigated. Bovine, equine, and porcine pericardium specimens served as controls. The donkey pericardium had a similar surface morphology to that of the control pericardia except for the wavy topology on both the fibrous and serous sides. The average thickness of donkey pericardium (ca. 120 µm) was significantly lower than that from bovine (375 µm) and equine (410 µm), but slightly higher than that from porcine (99 µm) specimens. The interlaced wavy collagen bundles in the pericardium were composed of collagen fibers about 100 nm in diameter. This unique structure ensures that the donkey pericardium has a comparable ultimate tensile strength (UTS) and a much higher failure strain than the commercial pericardia used for the manufacture of heart valves. The donkey pericardium has an organized wavy collagen bundle architecture similar to that of bovine pericardium and has a satisfactory UTS and high failure strain. The thin and strong donkey pericardium might be a good candidate valve leaflet material for TAVI.
Addressing data comparability in the creation of combined data sets of bioapatite carbon and oxygen isotopic compositions
Authors: Chesson L. A. , Kenyhercz M. W. , Regan L. A. , Berg G. E.
Published: May 2019

Before amalgamating published isotope data, comparability should be demonstrated. This paper compares carbon and oxygen isotopic compositions of 30 enamel samples measured by two laboratories. The aims were to see what, if any, isotopic variation was observed, to determine the causes as needed and to correct if possible. Bioapatite was acidified at 90°C in 2006 and at 26°C in 2017, while δ values were corrected via one‐point normalization in 2006 and by two‐point normalization in 2017. One case (of the 30) produced different δ values between the analysis dates, suggesting contamination. Repeated carbon isotope ratio measurements were not meaningfully different. Repeated oxygen isotope ratio measurements were significantly different, even following correction for acid‐carbonate fractionation at different temperatures and the renormalization of 2017 δ values using one point; however, differences were not meaningful for interpretations. Results were used to calculate real interpretative differences (RIDs) for comparing enamel bioapatite as 0.6‰ for δ13C values and as 1.6‰ for δ18O values.
Common Reducing Subspace Model and Network Alternation Analysis
Authors: Wang Wenjing , Zhang Xin , Li Lexin
Published: May 2019

Motivated by brain connectivity analysis and many other network data applications, we study the problem of estimating covariance and precision matrices and their differences across multiple populations. We propose a common reducing subspace model that leads to substantial dimension reduction and efficient parameter estimation. We explicitly quantify the efficiency gain through an asymptotic analysis. Our method is built upon and further extends a nascent technique, the envelope model, which adopts a generalized sparsity principle. This distinguishes our proposal from most xisting covariance and precision estimation methods that assume element-wise sparsity. Moreover, unlike most existing solutions, our method can naturally handle both covariance and precision matrices in a unified way, and work with matrix-valued data. We demonstrate the efficacy of our method through intensive simulations, and illustrate the method with an autism spectrum disorder data analysis.