bluuebunny/crossref_metadata_2025 huggingface dataset:
3-Carboxypyridinium chlorochromate  aluminium chloride  An efficient and inexpensive reagent system for the selective oxidation of sulfides to sulfoxides and sulfones in solution and under microwave irradiation
Authors: Mohammadpoor-Baltork Iraj , Memarian Hamid R , Bahrami Kiumars
Published: March 2005

3-Carboxypyridinium chlorochromate (CPCC) in the presence of aluminium chloride is a very efficient reagent for the selective oxidation of sulfides to sulfoxide and sulfones in solution and under microwave irradiation. It is noteworthy that different functional groups including carboncarbon double bonds, ketones, oximes, aldehydes, ethers, and acetals were tolerated under these reaction conditions.Key words: sulfoxides, sulfones, oxidation, chlorochromate, Lewis acid.
A novel enamination of β-dicarbonyl compounds catalyzed by Bi(TFA)3 immobilized on molten TBAB
Authors: Khodaei Mohammad M , Khosropour Ahmad R , Kookhazadeh Mehdi
Published: March 2005

Enamination of a wide variety of primary amines was successfully carried out in the presence of catalytic amounts of bismuth(III) trifluoroacetate immobilized on molten tetrabutylammonium bromide as "green" media under mild conditions. This new system of the catalyst is recyclable and reusable. Generally, the results of the reaction in tetrabutylammonium bromide is better than the previously obtained results in water because of their yields and reaction times.Key words: amine, bismuth compounds, β-dicarbonyl compounds, enamination, ionic liquid.
Efficient one-pot transformation of aminoarenes to haloarenes using halodimethylisulfonium halides generated in situ
Authors: Baik Woonphil , Luan Wanqiang , Lee Hyun Joo , Yoon Cheol Hun , Koo Sangho , Kim Byeong Hyo
Published: March 2005

Halodimethylsulfonium halide 1, which is readily formed in situ from hydrohaloic acid and DMSO, is a good nucleophilic halide. This activated nucleophilic halide rapidly converts aryldiazonium salt prepared in situ by the same hydrohaloic acid and nitrite ion to aryl chlorides, bromides, or iodides in good yield. The combined action of nitrite ion and hydrohaloic acid in DMSO is required for the direct transformation of aromatic amines, which results in the production of aryl halides within 1 h. Substituted compounds with electron-donating or -withdrawing groups or sterically hindered aromatic amines are also smoothly transformed to the corresponding aromatic halides. The only observed by-product is the deaminated arene (usually <7%). The isolated aryldiazonium salts can also be converted to the corresponding aryl halides using 1. The present method offers a facile, one-step procedure for transforming aminoarenes to haloarenes and lacks the environmental pollutants that usually accompany the Sandmeyer reaction using copper halides. Key words: aminoarenes, haloarenes, halodimethylsulfonium halide, halogenation, amination.
Efficient, microwave-assisted intramolecular 1,3-dipolar cycloadditions of oximes and N-methylnitrones derived from o-alkenylmethoxy-acetophenones
Authors: Singh Surinderjit , Ishar M PS , Singh Gajendra , Singh Rajinder
Published: March 2005

Contrary to literature reports, the o-allyloxy- and crotyloxy-acetophenone-oximes (2a, 2b) are transformed to nitrones, which undergo regio- and stereoselective intramolecular 1,3-dipolar cycloadditions, both on microwave heating under solvent free conditions and refluxing in toluene, to afford novel cycloadducts (5a, 5b); the oxazepine-N-oxide (3a) reported to be formed in 98% yield was obtained only as a minor product (~10%). However, o-cinnamyloxy-acetophenone-oxime (2c) under similar conditions undergoes intramolecular N-alkylation to afford nitrone (3c). The reactions carried out under microwave irradiation are cleaner, require shorter reaction times, and have higher yields. Corresponding intramolecular 1,3-dipolar cycloadditions of N-methylnitrones (B), generated in situ from o-alkenylmethoxy-acetophenones (1a1c) and N-methylhydroxylamine under solvent-free microwave irradiation conditions, are completely regio- and stereoselective, require much shorter reaction times, and afford adducts (4a4c) in higher yields, as compared with their thermal counterparts.Key words: cycloadditions, nitrones, oximes, o-alkenylmethoxy-acetophenones, microwave, isoxazolidines.
Effect of Astragalosides on Intracellular Calcium Overload in Cultured Cardiac Myocytes of Neonatal Rats
Authors: Meng Dan , Chen Xiang-Jian , Bian Yun-Yun , Li Ping , Yang Di , Zhang Ji-Nan
Published: March 2005

Astragalosides were the main active components from a native Chinese herb Astragalus membranaceus. Recent studies have shown that Astragalosides have a protective effect on myocardial injury in rats. The present study was designed to investigate the effect of Astragalosides on intracellular calcium overload and sarcoplasmic reticulum calcium load (SR Ca 2+ load) in cultured cardiac myocytes from neonatal rats. Astragalosides (100 μg/ml) were incubated in the presence of isoproterenol (ISO) (10-5 M) for 72 hours in cardiomyocytes. Metoprorol (10-6 M), a β1-selective antagonist, was cultured in the same condition as Astragalosides. The result showed that intracellular calcium concentration ([ Ca 2+] i ) and SR Ca 2+ load increased in ISO-treated cardiac myocytes as compared to control (P<0.01). Astragalosides prevented ISO-induced increase in [ Ca 2+] i and SR Ca 2+ load. Metoprolol also inhibited those increase. The mRNA expression and activity of sarcoplasmic reticulum Ca 2+ ATPase (SERCA) were enhanced following ISO treatment in cardiac myocytes, and these increases were inhibited by Astragalosides or metoprolol (P<0.05). The decrease of superoxide dismutase (SOD) activity and the elevation of intracellular maleic dialdehyde (MDA) were observed after ISO treatment in cardiac myocytes. Both Astragalosides and metoprolol restored the SOD activity and reduced the level of MDA. We conclude that Astragalosides have the effects on reducing [ Ca 2+] i and SR Ca 2+ load, enhancing free radical removal and decreasing lipid peroxidation in ISO-treated cardiomyocytes, which might account for their protective effect on myocardial injury.
Yukmijihwang-Tang Derivatives Enhance Cognitive Processing in Normal Young Adults: A Double-Blinded, Placebo-Controlled Trial
Authors: Park Eunhye , Kang Moonkyu , Oh Jung-Wan , Jung Myungsuk , Park Changbeom , Cho Chongwoon , Kim Changsook , Ji Sangeun , Lee Youngheuk , Choi Hyun , Kim Hongyeoul , Ko Seonggyu , Shin Minkyu , Park Seongkyu , Kim Hyun-Taek , Hong Moochang , Bae Hyunsu
Published: March 2005

Yukmijihwang-tang (YMJ), also known as Luweidihuang-tang in China, has been widely used as a general herbal tonic for hundreds of years in many Asian countries. This study examines whether YMJ derivatives (YMJd) enhance cognitive ability in normal human subjects and discusses its potential as treatment for dementia patients with deficient cognitive ability. Subjects were divided into two groups, the placebo-treated group (n=15) and the YMJd-treated group (n=20). K-WAIS tests, a Korean version of an individual intelligence quotient (IQ) test, and a P300 latency assessment of event-related potential (ERP) were conducted in order to measure changes in cognitive ability before and after 6 weeks of YMJd treatment. The K-WAIS mean scores of the group treated with YMJd were significantly higher than those of the placebo group (p<0.05), and their mean P300 latency was substantially shorter (p<0.005). These results suggest that YMJd treatment accelerates the speed of information processing and enhances cognitive ability. YMJd treatment may help dementia patients or the elderly recover from cognition deficiencies or degeneration in clinic.
Acupuncture Pain Management for Patients with Cystic Fibrosis: A Pilot Study
Authors: Lin Yuan-Chi , Ly Helen , Golianu Brenda
Published: March 2005

Cystic fibrosis is the most commonly occurring genetic disorder among Caucasians. The purpose of this study was to evaluate the effects of acupuncture for pain management in patients with cystic fibrosis; clinical data was obtained regarding the integration of acupuncture into the management of pain in patients with cystic fibrosis. Visual analog scales were used for pain measurement. Acupuncture was found to be effective in decreasing pain complaints in patients with cystic fibrosis. No side effects or complications were reported in relation to the acupuncture treatment. Acupuncture is effective for managing symptoms and illnesses associated with cystic fibrosis. Further randomized controlled trials will be necessary to evaluate additional efficacy in pain management and the improvement of the quality of life of patients with cystic fibrosis.
Increase in the Vagal Modulation by Acupuncture at Neiguan Point in the Healthy Subjects
Authors: Huang Sheng-Teng , Chen Gau-Yang , Lo Huey-Ming , Lin Jaung-Gang , Lee Yin-Shiung , Kuo Cheng-Deng
Published: March 2005

Acupuncture at the Neiguan (P6) point has been shown to lessen nausea and vomiting which are related to vagal modulation. This study investigated whether acupuncture at the P6 point could improve vagal modulation by using heart rate variability analysis. We compared the heart rate variability measures of 39 subjects receiving acupuncture at the P6 point, 38 subjects receiving sham acupuncture, and 34 subjects receiving no treatment at all. The normalized high-frequency power was used as the index of vagal modulation, and the low-/high-frequency power ratio was used as the index of sympathovagal balance. The normalized high-frequency power after acupuncture increased significantly from 28.1±12.6 nu (mean±SD) to 30.7±14.1 nu in the P6 acupuncture group, but not in the sham acupuncture (30.6±13.7 nu versus 31.8±13.8 nu) or no-treatment group (30.1±15.0 nu versus 30.1±15.7 nu). In both the P6 and sham acupuncture groups, the mean RR interval (the intervals between consecutive R waves in the electrocardiogram) increased significantly after acupuncture. In the no-treatment group, there was no statistical difference in all heart rate variability measures in the initial and later sessions. In conclusion, acupuncture at the P6 point can increase vagal modulation of the subjects. This result may be helpful in the understanding of the mechanism underlying the effect of acupuncture or acupressure at P6 on the lessening of nausea and vomiting in clinic.
Arginine vasopressin stimulation of cerebral microvascular endothelial cell Na-K-Cl cotransporter activity is V1 receptor and [Ca] dependent
Authors: O’Donnell Martha E. , Duong Vicki , Suvatne Jimmy , Foroutan Shahin , Johnson Denise M.
Published: March 2005

Ischemia-induced brain edema formation is mediated by increased transport of Na and Cl across an intact blood-brain barrier (BBB). Our previous studies have provided evidence that a luminally located BBB Na-K-Cl cotransporter is stimulated during cerebral ischemia to increase transport of Na and Cl into the brain. The main focus of the present study was to evaluate the effects of arginine vasopressin (AVP), previously shown to be increased in the brain during ischemia and to promote edema formation, on activity of the BBB cotransporter. Cerebral microvascular endothelial cell (CMEC) monolayers were cultured in astroglial cell conditioned medium, and Na-K-Cl cotransporter activity was assessed as bumetanide-sensitive 86Rb influx. In both human and bovine CMECs, as well as in freshly isolated microvessels, AVP stimulated cotransport activity. This stimulatory effect was mimicked by V1 but not V2 vasopressin agonists and was blocked by V1 but not V2 vasopressin antagonists. Consistent with a V1 vasopressin receptor mechanism of action, AVP caused an increase in CMEC intracellular [Ca] that was blocked by a V1 antagonist. Exposing the cells to [Ca]-free media and/or reducing intracellular [Ca] by BAPTA also blocked AVP stimulation of CMEC cotransporter activity, as did the phospholipase C inhibitor U-73122. Finally, we found that while stimulation of CMEC cotransporter activity by AVP occurred within minutes, it was also sustained for hours in the continued presence of AVP. These findings support the hypothesis that AVP, through a V1 receptor- and [Ca]-dependent mechanism, stimulates the BBB Na-K-Cl cotransporter to participate in ischemia-induced edema formation.
HAI-1 regulates activation and expression of matriptase, a membrane-bound serine protease
Authors: Oberst Michael D. , Chen Li-Yuan L. , Kiyomiya Ken-Ichi , Williams Cicely A. , Lee Ming-Shyue , Johnson Michael D. , Dickson Robert B. , Lin Chen-Yong
Published: March 2005

Hepatocyte growth factor activator inhibitor-1 (HAI-1) was initially identified as cognate inhibitor of matriptase, a membrane-bound serine protease. Paradoxically, HAI-1 is also required for matriptase activation, a process that requires sphingosine 1-phosphate (S1P)-mediated translocation of the protease to cell-cell junctions in human mammary epithelial cells. In the present study, we further explored how HAI-1 regulates this protease. First, we observed that after S1P treatment HAI-1 was cotranslocated with matriptase to cell-cell junctions and that the cellular ratio of HAI-1 to matriptase was maintained during this process. However, when this ratio was changed by cell treatment with HAI-1 small interfering RNA or anti-HAI-1 MAb M19, spontaneous activation of matriptase occurred in the absence of S1P-induced translocation; S1P-induced matriptase activation was also enhanced. These results support a role for HAI-1 in protection of cell from uncontrolled matriptase activation. We next expressed matriptase, either alone or with HAI-1 in breast cancer cells that do not endogenously express either protein. A defect in matriptase trafficking to the cell surface occurred if wild-type matriptase was expressed in the absence of HAI-1; this defect appeared to result from matriptase toxicity to cells. Coexpression with matriptase of wild-type HAI-1, but not HAI-1 mutants altered in its Kunitz domain 1, corrected the trafficking defect. In contrast, catalytically defective matriptase mutants were normal in their trafficking in the absence of HAI-1. These results are also consistent with a role for HAI-1 to prevent inappropriate matriptase proteolytic activity during its protein synthesis and trafficking. Taken together, these results support multiple roles for HAI-1 to regulate matriptase, including its proper expression, intracellular trafficking, activation, and inhibition.
RNA interference targeted to multiple P2X receptor subtypes attenuates zinc-induced calcium entry
Authors: Liang Lihua , Zsembery Akos , Schwiebert Erik M.
Published: March 2005

A postulated therapeutic avenue in cystic fibrosis (CF) is activation of Ca2+-dependent Cl− channels via stimulation of Ca2+ entry from extracellular solutions independent of CFTR functional status. We have shown that extracellular zinc and ATP induce a sustained increase in cytosolic Ca2+ in human airway epithelial cells that translates into stimulation of sustained secretory Cl− transport in non-CF and CF human and mouse airway epithelial cells, cell monolayers, and nasal mucosa. On the basis of these studies, the Ca2+ entry channels most likely involved were P2X purinergic receptor channels. In the present study, molecular and biochemical data show coexpression of P2X4, P2X5, and P2X6 subtypes in non-CF (16HBE14o−) and CF (IB3-1) human bronchial epithelial cells. Other P2X receptor Ca2+ entry channel subtypes are expressed rarely or not at all in airway epithelia, epithelial cell models from other CF-relevant tissues, or vascular endothelia. Novel transient lipid transfection-mediated delivery of small interference RNA fragments specific to P2X4 and P2X6 (but not P2X5) into IB3-1 CF human airway epithelial cells inhibited extracellular zinc- and ATP-induced Ca2+ entry markedly in fura-2 Ca2+ measurements and “knocked down” protein by >65%. These data suggest that multiple P2X receptor Ca2+ entry channel subtypes are expressed in airway epithelia. P2X4 and P2X6 may coassemble on the airway surface as targets for possible therapeutics for CF independent of CFTR genotype.
Reduction of Common Synaptic Drive to Ankle Dorsiflexor Motoneurons During Walking in Patients With Spinal Cord Lesion
Authors: Hansen N. L. , Conway B. A. , Halliday D. M. , Hansen S. , Pyndt H. S. , Biering-Sørensen F. , Nielsen J. B.
Published: March 2005

It is possible to obtain information about the synaptic drive to motoneurons during walking by analyzing motor-unit coupling in the time and frequency domains. The purpose of the present study was to compare motor-unit coupling during walking in healthy subjects and patients with incomplete spinal cord lesion to obtain evidence of differences in the motoneuronal drive that result from the lesion. Such information is of importance for development of new strategies for gait restoration. Twenty patients with incomplete spinal cord lesion (SCL) participated in the study. Control experiments were performed in 11 healthy subjects. In all healthy subjects, short-term synchronization was evident in the discharge of tibialis anterior (TA) motor units during the swing phase of treadmill walking. This was identified from the presence of a narrow central peak in cumulant densities constructed from paired EMG recordings and from the presence of significant coherence between these signals in the 10- to 20-Hz band. Such indicators of short-term synchrony were either absent or very small in the patient group. The relationship between the amount of short-term synchrony and the magnitude of the 10- to 20-Hz coherence in the patients is discussed in relation to gait ability. It is suggested that supraspinal drive to the spinal cord is responsible for short-term synchrony and coherence in the 10- to 20-Hz frequency band during walking in healthy subjects. Absence or reduction of these features may serve as physiological markers of impaired supraspinal control of gait in SCL patients. Such markers could have diagnostic and prognostic value in relation to the recovery of locomotion in patients with central motor lesions.
Control of Locomotor Cycle Durations
Authors: Yakovenko S. , McCrea D. A. , Stecina K. , Prochazka A.
Published: March 2005

In intact animals and humans, increases in locomotor speed are usually associated with decreases in step cycle duration. Most data indicate that the locomotor central pattern generator (CPG) shortens cycle duration mainly by shortening the durations of extensor rather than flexor phases of the step cycle. Here we report that in fictive locomotion elicited by electrical stimulation of the midbrain locomotor region (MLR) in the cat, spontaneous variations in cycle duration were due more to changes in flexor rather than extensor phase durations in 22 of 31 experiments. The locomotor CPG is therefore not inherently extensor- or flexor-biased. We coined the term “dominant” to designate the phase (flexion or extension) showing the larger variation. In a simple half-center oscillator model, experimental phase duration plots were fitted well by adjusting two parameters that corresponded to background drive (“bias”) and sensitivity (“gain”) of the oscillator's timing elements. By analogy we argue that variations in background drive to the neural timing elements of the CPG could produce larger variations in phase duration in the half-center receiving the lower background drive, i.e., background drive may determine which half-center is dominant. The fact that data from normal cats were also fitted well by the model indicates that sensory input and central drive combine to determine locomotor phase durations. We conclude that there is a considerable flexibility in the control of phase durations in MLR-induced fictive locomotion. We posit that this may be explained by changes in background excitation of neural timing elements in the locomotor CPG.
Correlation test to assess low-level processing of high-density oligonucleotide microarray data
Authors: Ploner Alexander , Miller Lance D , Hall Per , Bergh Jonas , Pawitan Yudi
Published: March 2005

Background

There are currently a number of competing techniques for low-level processing of oligonucleotide array data. The choice of technique has a profound effect on subsequent statistical analyses, but there is no method to assess whether a particular technique is appropriate for a specific data set, without reference to external data.

Results

We analyzed coregulation between genes in order to detect insufficient normalization between arrays, where coregulation is measured in terms of statistical correlation. In a large collection of genes, a random pair of genes should have on average zero correlation, hence allowing a correlation test. For all data sets that we evaluated, and the three most commonly used low-level processing procedures including MAS5, RMA and MBEI, the housekeeping-gene normalization failed the test. For a real clinical data set, RMA and MBEI showed significant correlation for absent genes. We also found that a second round of normalization on the probe set level improved normalization significantly throughout.

Conclusion

Previous evaluation of low-level processing in the literature has been limited to artificial spike-in and mixture data sets. In the absence of a known gold-standard, the correlation criterion allows us to assess the appropriateness of low-level processing of a specific data set and the success of normalization for subsets of genes.
Pathways to care and ethnicity. 2: Source of referral and help-seeking
Authors: Morgan Craig , Mallett Rosemarie , Hutchinson Gerard , Bagalkote Hemant , Morgan Kevin , Fearon Paul , Dazzan Paola , Boydell Jane , McKenzie Kwame , Harrison Glynn , Murray Robin , Jones Peter , Craig Tom , Leff Julian
Published: March 2005

Background

Previous research has found that African–Caribbean and Black African patients are likely to come into contact with mental health services via more negative routes, when compared with White patients. We sought to investigate pathways to mental health care and ethnicity in a sample of patients with a first episode of psychosis drawn from two UK centres.

Method

We included all White British, other White, African–Caribbean and Black African patients with a first episode of psychosis who made contact with psychiatric services over a 2-year period and were living in defined areas. Clinical, socio-demographic and pathways to care data were collected from patients, relatives and case notes.

Results

Compared with White British patients, general practitioner referral was less frequent for both African–Caribbean and Black African patients and referral by a criminal justice agency was more common. With the exception of criminal justice referrals for Black African patients, these findings remained significant after adjusting for potential confounders.

Conclusions

These findings suggest that factors are operating during a first episode of psychosis to increase the risk that the pathway to care for Black patients will involve non-health professionals.
Occult suicidality in an emergency department population
Authors: Claassen Cynthia A. , Larkin Gregory L.
Published: March 2005

The prevalence and severity of suicidal ideation was established in a patient sample seeking emergency treatment for non-psychiatric reasons. Using a computerised mental health screening panel, data were collected from waiting-room patients during randomised shifts over a 45-day period. Of 1590 screened patients, 185 (11.6%) acknowledged suicidal ideation and 31 (2%) reported planning to kill themselves. Almost all of those with suicidal ideation (97%) acknowledged symptoms consistent with mood, anxiety and/or substance-related disorders. Structured medical record review revealed that 25 of the 31 patients planning suicide were undetected during their index visit, and that 4 attempted suicide within 45 days of the visit. All survived.
Heroin prescription: a limited but valuable role
Authors: Carnwath Tom
Published: March 2005

Luty (2005, this issue) performs a valuable service by directing attention to the new guidelines from the National Treatment Agency. He explains clearly why he thinks these guidelines are wrong. I was a member of the expert advisory group that produced the guidelines, and will therefore attempt to defend them (my arguments do not necessarily reflect the views of all members of the group).
The College
Authors: Bouch Joe , Jackson Robert
Published: March 2005

In April 2001 the College introduced personal development plans (PDPs) as the mechanism for achieving continuing professional development (CPD) objectives. We moved from an individual, retrospective points counting exercise to a prospective peer-group based activity centring on individuals' learning objectives (Royal College of Psychiatrists, 2001). The current CPD policy is due for review in 2005. It is largely in line with General Medical Council guidance, Continuing Professional Development (April 2004) and the Academy of Medical Royal Colleges, CPD: The Ten Principles. A Framework for Continuing Professional Development (February 2002), and major revision will not be necessary. Two significant changes will be incorporated in the new policy. The first is an audit procedure whereby a random 5% of returns will be subject to further scrutiny. This is a process audit and necessary for the quality assurance of the system as a whole (Bouch & Jackson, 2004). The second will allow us to complete up to 10 h of our 50-h minimum requirement for attending meetings, by engaging in online CPD activities.
TheBLADE ON PETIOLEgenes act redundantly to control the growth and development of lateral organs
Authors: Norberg Mikael , Holmlund Mattias , Nilsson Ove
Published: March 2005

Developmental processes in multicellular organisms involve an intricate balance between mechanisms that promote cell division activity and growth, and others that promote cell differentiation. Leaf development in Arabidopsis thaliana is controlled by genes like BLADE ON PETIOLE1(BOP1), which prevent the development of ectopic meristematic activity that leads to the formation of new organs, and JAGGED(JAG), which control the proximodistal development of the leaf by regulating cell-division activity. We have isolated and characterized the BOP1 gene together with a functionally redundant close homolog that we name BOP2. The BOP genes are members of a gene family containing ankyrin repeats and a BTB/POZ domain, suggesting a role in protein-protein interaction. We show that the BOP genes are expressed in the proximal parts of plant lateral organs where they repress the transcription not only of class 1 knox genes but also of JAG. We also show that the BOP genes are acting together with the flower meristem identity gene LEAFY in the suppression of bract formation. These findings show that the BOP genes are important regulators of the growth and development of lateral organs.
Evolution of gap junction proteins – the pannexin alternative
Authors: Panchin Yuri V.
Published: March 2005

Gap junctions provide one of the most common forms of intercellular communication. They are composed of membrane proteins that form a channel that is permeable to ions and small molecules, connecting the cytoplasm of adjacent cells. Gap junctions serve similar functions in all multicellular animals(Metazoa). Two unrelated protein families are involved in this function;connexins, which are found only in chordates, and pannexins, which are ubiquitous and present in both chordate and invertebrate genomes. The involvement of mammalian pannexins to gap junction formation was recently confirmed. Now it is necessary to consider the role of pannexins as an alternative to connexins in vertebrate intercellular communication.
Prey-capture in Pomacanthus semicirculatus (Teleostei,Pomacanthidae): functional implications of intramandibular joints in marine angelfishes
Authors: Konow Nicolai , Bellwood David R.
Published: March 2005

We examined prey-capture morphology and kinematics in the angelfish, Pomacanthus semicirculatus (Cuvier 1931), to evaluate the magnitude and role of functional specialisation. The feeding apparatus of P. semicirculatus possess three biomechanical mechanisms of particular interest: (1) a novel intramandibular joint, permitting dentary rotation and protruded jaw closure; (2) an opercular linkage facilitating mandible depression; and (3) a suspensorial linkage with two novel points of flexion,permitting anterior rotation of the suspensorium and augmenting mandible protrusion. Prey-capture kinematics were quantified using motion analysis of high-speed video, yielding performance profiles illustrating timing of onset,duration and magnitude of movement in these three biomechanical systems, and other variables traditionally quantified in studies of teleostean ram–suction feeding activity. Mandible depression and suspensorial rotation both augmented mandible protrusion, and coincided during jaw protrusion, typically increasing head length by 30%. Jaw closure appeared to result from contraction of the adductor mandibulae segment A2, which rotated the dentary by approximately 30° relative to the articular. This resulted in jaw closure with the mandible fully depressed and the jaws at peak-protrusion. Feeding events were concluded by a high-velocity jaw retraction (20–50 ms), and completed in 450–750 ms. Feeding kinematics and morphology of Pomacanthus differed from other biting teleosts, and more closely resemble some long-jawed ram–suction feeders. The structural and functional modifications in the Pomacanthusfeeding apparatus are matched to an unusual diet of structurally resilient and firmly attached benthic prey.
Tuning of host plants with vibratory songs ofNezara viridulaL(Heteroptera: Pentatomidae)
Authors: Čokl Andrej , Zorović Maja , Žunič Alenka , Virant-Doberlet Meta
Published: March 2005

Songs of the southern green stink bug Nezara viridula L, recorded on a non-resonant loudspeaker membrane, reflect frequency characteristics of body vibrations. The song dominant frequency directly depends on the repetition rate of potentials recorded from synchronously contracting muscles that vibrate the abdomen during singing. Spectra of naturally emitted signals recorded on the pronotum of a singing bug or on a plant contain peaks characteristic of plant resonant spectra. The dominant resonant frequency of sound-induced vibrations in bean and other stink bug host plants ranges between 160 and 215 Hz and subdominant peaks do not exceed 600 Hz. There is no correlation between spectral peak positions and recording points on a plant. The dominant resonance peak corresponds to the best frequency sensitivity of N. viridula middle frequency subgenual receptor cell. Subdominant peaks around and below 100 Hz lie close to the dominant frequency of body vibrations during singing and to the range of best frequency sensitivity of low frequency receptor cells. Tuning of plant resonant frequencies with spectral properties of songs and frequency sensitivity of sensory organs is discussed in the context of stink bug substrate-borne communication.
The ontogenetic changes in the thermal properties of blubber from Atlantic bottlenose dolphinTursiops truncatus
Authors: Dunkin Robin C. , McLellan William A. , Blum James E. , Pabst D. Ann
Published: March 2005

In Atlantic bottlenose dolphins Tursiops truncatus, both the thickness and lipid content of blubber vary across ontogeny and across individuals of differing reproductive and nutritional status. This study investigates how these changes in blubber morphology and composition influence its thermal properties. Thermal conductivity (W m–1deg.–1, where deg. is °C) and thermal insulation(m2 deg. W–1) of dolphin blubber were measured in individuals across an ontogenetic series (fetus through adult, N=36),pregnant females (N=4) and emaciated animals (N=5). These thermal properties were determined by the simultaneous use of two common experimental approaches, the heat flux disc method and the standard material method. Thickness, lipid and water content were measured for each blubber sample. Thermal conductivity and insulation varied significantly across ontogeny. Blubber from fetuses through sub-adults was less conductive(range=0.11–0.13±0.02 W m–1deg.–1) than that of adults (mean=0.18 W m–1deg.–1). The conductivity of blubber from pregnant females was similar to non-adult categories, while that of emaciated animals was significantly higher (0.24 ± 0.04 W m deg.–1) than all other categories. Blubber from sub-adults and pregnant females had the highest insulation values while fetuses and emaciated animals had the lowest. In nutritionally dependant life history categories, changes in blubber's thermal insulation were characterized by stable blubber quality (i.e. conductivity)and increased blubber quantity (i.e. thickness). In nutritionally independent animals, blubber quantity remained stable while blubber quality varied. A final, unexpected observation was that heat flux measurements at the deep blubber surface were significantly higher than that at the superficial surface, a pattern not observed in control materials. This apparent ability to absorb heat, coupled with blubber's fatty acid composition, suggest that dolphin integument may function as a phase change material.
An analysis of diagnostic delay in unilateral facial paralysis
Authors: Alaani A , Hogg R , Saravanappa N , Irving R M
Published: April 2005

Bell’s palsy or idiopathic facial palsy is the commonest cause of unilateral lower motor neuron facial palsy. Misdiagnosis of facial nerve palsy as Bell’s palsy is still seen in clinical practice. The clinician should always consider the possibility of a potentially serious underlying pathology before making the diagnosis of Bell’s palsy.

We present a series of 13 patients referred to our ENT department with an initial diagnosis of Bell’s palsy. Further clinical examination and investigation revealed the underlying cause. Many had additional symptoms and signs related to the ear.

In all patients with unilateral facial palsy a detailed history should be taken and thorough clinical examination carried out. Where no recovery occurs within the expected time period further radiological investigations such as computerized axial tomography (CT) and magnetic resonance imaging (MRI) should be performed. Current scanning techniques provide good quality images, which can show occult lesions of the temporal bone, internal acoustic canal and/or cerebellopontine angle. Radiologists with a special interest and experience in otoneurological radiology should ideally report these images, and a close co-operation between ENT surgeon and radiologist is essential in arriving at a proper diagnosis.
Thoracic ranula: an extremely rare case
Authors: Pang Claudine Elizabeth , Lee Tee Sin , Pang Kenny Peter , Pang Yoke Teen
Published: April 2005

We present the first case of a thoracic ranula which originated from the left submandibular area extending into the subcutaneous tissue planes of the anterior chest wall. The patient had a history of surgery for a previous benign left salivary gland cyst, and presented with an enlarging mass in the anterior chest wall. This was a recurrence of a ranula, with an extension into the anterior thoracic wall. The thoracic ranula was excised, together with ipsilateral sublingual and submandibular glands, via a transcervical approach. No recurrence was detected over a 3-year post-operative follow up.
A review of ENT consultant postal questionnaires
Authors: Ramphul N , Saunders J , Ahmed I , Fenton J E
Published: April 2005

In recent years, there appears to have been an increase in the number of postal questionnaires being received by ENT consultants. Questionnaires with unsound methodology waste the time of those who send and receive them, as inferences cannot be made from their results. In this study, a review was performed on a sample of 19 questionnaire studies published in two ENT journals between January 1998 and December 2002. Each study was given a 30-point score, based on the quality of its methodology. The average score assigned to each study was 32 per cent, suggesting that the quality of methodology was generally poor. These results should serve as a warning to those embarking on and those interpreting research of this kind.
Posterior Segment Changes in Gaucher Disease
Authors: Mueller Anna , Chang Ta Chen Peter
Published: February 2024

This case report discusses posterior segment characteristics in a patient aged 24 years with low vision and a history of Gaucher disease.
Design and Development of Sustainable Polyurethane Foam: A Proof‐of‐Concept as Customizable Packaging for Cultural Heritage Applications
Authors: Pascarella Annachiara , Recupido Federica , Lama Giuseppe Cesare , Sorrentino Luigi , Campanile Assunta , Liguori Barbara , Berthet Mathéo , Rollo Gennaro , Lavorgna Marino , Verdolotti Letizia
Published: February 2024

World cultural heritage is an invaluable resource and must be preserved. Conventional packaging materials, such as polystyrene, polyurethane (PU) foams, paper‐like tissue, and nylon fabric, are petrol‐based. According to Agenda 2030, and Green Deal directives, the development of alternative bio‐based materials has gained significant scientific and technological relevance. In this scenario, a new sustainable packaging material for safe storage and transporting of the artifacts, based on a sustainable PU foam filled with 6 wt% of Zeolite 4A, is proposed. Zeolite 4A dispersion into PU matrix imparts higher compression strength and energy absorption capability with respect to unloaded materials (7‐fold increase), owing to its remarkable cell nucleating effect. The proposed foam can adsorb water vapor, up to 29 wt%, thus contributing to regulating the humidity around the artifact. A remarkable advantage of the developed foam is the possibility to use it as plaster for precisely casting the article to protect, resulting in strongly improved handling, protection, and transportation safety with respect to conventional packaging solutions. A proof‐of‐concept workflow is proposed, consisting of: 1) three‐dimensional (3D) scanning of the original artifact; 2) its replication through 3D printing; 3) the use of such a replica to prepare a tailored cast of the artifact shape.
Advancing Robotic Gripper Control with the Integration of Flexible Printed Pressure Sensors
Authors: Takeda Yasunori , Wang Yi‐Fei , Yoshida Ayako , Sekine Tomohito , Kumaki Daisuke , Tokito Shizuo
Published: February 2024

A pressure sensor array fabricated on a flexible substrate using the printing method can be easily mounted in various locations by combining it with a wireless communication circuit. This is particularly advantageous for applications where wiring is difficult, such as the tip of a robot gripper. Additionally, printed devices are highly efficient in material utilization, facilitating large areas and low‐cost production. In recent years, there has been growing interest in the harmonious interaction between robots and humans, and the intelligentization of robots is a worthwhile research area. Herein, a method for realizing intelligent gripping force control and object recognition robots using a printed flexible pressure sensor array in combination with a wireless communication circuit is proposed. By mounting the sensor array on a robot gripper and performing arithmetic processing on software, these capabilities are achieved.
Stimulation of Sympathetic Nerve Using Ultraflexible Cuff Electrodes Inhibits Inflammation Caused by Tendon Rupture
Authors: Tu Kejun , Bao Rong , Wang Longchun , Guo Zhejun , Jiang Chunpeng , Su Yuezeng , Liu Shen , Liu Jingquan
Published: February 2024

Inflammation is a serious symptom of tendon rupture, that leads to peritendinous adhesion and damages the recovery of motor function. Although it is commonly treated using medical therapy, side effects occur in the cardiovascular and genitourinary systems. Electrical stimulation (ES) is a novel alternative to neural modulation. However, its inflammatory inhibitory effect around the tendon has rarely been investigated. Here, an ultraflexible cuff electrode with a thickness of only 3.3 µm is constructed to conformally wrap around the sympathetic chain ganglia for both stimulation and recording. The superior pliancy of the electrode causes less damage to the surrounding tissues and enhances the efficiency of stimulation and recording. ES reduces inflammation‐related factors of IL‐6 and IL‐1β by 51.6% and 19.8%, respectively, and increases the sympathetic nerve neurotransmitter of norepinephrine (NE) by almost an order of magnitude in the ruptured tendon. Sympathetic recordings have also demonstrated that more neural activity is evoked after ES. To the best of the knowledge, this study is the first to verify that the ES of the sympathetic nerve can inhibit inflammation around the tendon, bringing new inspiration for the tendon rupture treatment.
Pathology‐Guided Cell Membrane‐Coated Polydopamine Nanoparticles for Efficient Multisynergistic Treatment of Periodontitis
Authors: Pan Shengyuan , Zhong Wenjie , Lan Yuyan , Yu Simin , Yang Lanxin , Yang Fengze , Li Jinda , Gao Xiang , Song Jinlin
Published: February 2024

Nanodrug delivery systems (NDDSs) for the treatment of periodontitis remain a significant clinical challenge. The low biocompatibility, singular effect, and lack of disease specificity of conventional nanoparticles limit their biomedical application. Recent studies have highlighted the pivotal role of cell membrane‐coated nanotechnology in anti‐inflammatory strategies due to its improved biocompatibility and superior biointerface properties. Herein, combined with the pathological heterogeneity of periodontitis revealed by bioinformatics analysis, this study develops a novel cell membrane‐coated nanoparticle composed of two elements: minocycline‐loaded polydopamine nanoparticles (PM) and cRGD‐modified cell membrane (RCM). The in vitro results indicate that PM@RCM rescues impaired human periodontal ligament stem cells through antioxidant, anti‐ferroptosis, anti‐inflammatory, and pro‐osteogenic effects, and exhibits favorable antibacterial bioactivity. The in vivo studies further reveal that PM@RCM promotes periodontal tissue regeneration and remodeled periodontal homeostasis in periodontitis mice. Collectively, these findings highlight the unique pathological changes in periodontitis. Moreover, the novel NDDS developed in this study, which leverages the excellent natural properties of cell membrane‐coated nanotechnology and the versatility of nanoparticle cores, provides a promising strategy for the clinical treatment of periodontitis.
Enhanced Strength for Double Network Hydrogel Adhesive Through Cohesion‐Adhesion Balance
Authors: Song Rijian , Wang Xiaoyu , Johnson Melissa , Milne Cameron , Lesniak‐Podsiadlo Anna , Li Yinghao , Lyu Jing , Li Zishan , Zhao Chunyu , Yang Lizhu , Lara‐Sáez Irene , A Sigen , Wang Wenxin
Published: February 2024

Hydrogel adhesives exhibit great potential in various biomedical fields such as tissue sealing and soft robotics. However, the high‐water content and defective network structures of these hydrogel adhesives result in low intrinsic mechanical strength, severely impeding their application. In this study, it is reported that the strong hydrogel adhesive strength can be achieved when a balance is established between adhesive forces and cohesive forces. Based on this principle, a new double network (DN) design is created to combine an adhesive polyvinyl alcohol (PVA) as the first network with a flexible sodium alginate (SA) as the second network. A delicate balance is achieved between cohesion and adhesion by adjusting the ratio between the first rigid adhesive network and the second flexible network. As a result, this balanced DN hydrogel adhesive exhibits a strong tissue adhesion strength, approximately three times higher than that in the non‐balance situation.
Semi‐Solid Thermo‐Electrochemical Cell Based Wearable Power Generator for Body Heat Harvesting
Authors: Xu Tian , Tao Yang , Qian Yongxin , Li Wang , Ma Zheng , Zheng Zusheng , Li Xin , Jiang Qinghui , Luo Yubo , Yang Junyou
Published: February 2024

A high‐performance TiO2/GO‐Fe3+/2+‐based N‐type thermo‐electrochemical cell (TEC) is demonstrated and prepared P‐N combined power generator prototypes combining BST/FeCN3‐/4−‐based P‐type TEC that is reported in the previous work. The functional groups on the TiO2/GO in the electrolyte can adjust the solvation structure of Fe3+/2+to improve theSevalue. In addition, TiO2acts as a relay reaction site to improve charge transport efficiency. The synergistic effect results inSeof 2.53 mV K−1and a maximum output power density of 0.38 W m−2. Besides, both flexible and rigid power generator prototypes are prepared. It is worth noting that the flexible prototype can generate an open‐circuit voltage of 1.27 V and power an electronic watch by using the temperature difference between the human body and the environment. This work demonstrates the potential of TEC for low‐grade heat energy recovery and powering wearable devices.
Engineering Ferroelectric‐/Ion‐Modulated Conductance in 2D vdW CuInP2S6 for Non‐Volatile Digital Memory and Artificial Synapse
Authors: Ci Wenjuan , Wang Peng , Xue Wuhong , Yuan Hongtao , Xu Xiaohong
Published: February 2024

Two‐dimensional (2D) ferroionics is appealing in performing complex artificial intelligence tasks due to the interesting property of coexistence of ferroelectricity and ionic activities. CuInP2S6 (CIPS), as a typical 2D ferroionic material, is highly conducive to rich functions of information devices due to the displacement of Cu+ inducing both ferroelectricity and ionic conductivity. However, the coupling and modulation of polarization and ion migration in CIPS for multifunctional information devices has not been fully explored. Here, this study demonstrates that digital memory and synaptic simulations are realized in Au/CIPS/Au device via engineering ferroelectric polarization reversal and the long‐distance migration of the Cu+ to change conductive modes. Steep resistive switching behavior based on ion‐migration is observed with a high on/off ratio of over 108, long retention time (>2 × 104 s), and current compliance engineered multilevel resistance states, demonstrating reliable nonvolatile high‐density memory characteristics. Based on the continuous modulation of polar order, the key synaptic behaviors are successfully simulated. Moreover, by the co‐modulation of polarization state and ions migration, the paired‐pulse facilitation, paired‐pulse depression, and potentiation following depression are achieved. These results suggest that CIPS is a promising candidate for constructing high‐performance, function‐enriched devices for data storage, information processing, and neuromorphic computing.
Photoactivation Inducing Multifunctional Coupling of Fluorophore for Efficient Tumor Therapy In Situ
Authors: Zhang Weijie , Lv Yunxia , Huo Fangjun , Yun Yang , Yin Caixia
Published: February 2024

Photoactivatable molecules, with high‐precision spatialtemporal control, have largely promoted bioimaging and phototherapy applications of fluorescent dyes. Here, the first photoactivatable sensor (BI) is described that can be triggered by broad excitation light (405–660 nm), which further undergoes intersystem crossing and H‐atom transfer processes to forming superoxide anion radicals (O2−•) and carbon radicals. Particularly, the photoinduced gain of carbon‐centered radicals (BI•) allows for radical‐radical coupling to afford the combined crosslink product (BI─BI), which would be oxidized in the presence of O2−• to produce an extended conjugate system with near infrared emission (820 nm). Besides, the photochemically generated product (Cy─BI) possesses ultra‐high photothermal conversion efficiency up to 90.9%, which optimized phototherapy potential. What′s more, Western Blot assay reveals that both BI and the photoproduct Cy─BI can efficiently inhibit the expression of CHK1, and the irradiation of BI and Cy─BI further induces apoptosis and ultimately enhances the phototherapeutic effects. Thus, the combination of cell cycle block inducing apoptosis, photodynamic therapy and photothermal therapy treatments significantly suppress solid tumor in vivo antitumor efficacy explorations. This is a novel finding in developing photoactivatable molecules, as well as the broad applicability of photoimaging and phototherapy in tumor‐related areas.