bluuebunny/crossref_metadata_2025 huggingface dataset:
LITERATURE REVIEW: ADVANTAGES AND DISADVANTAGES OF BLACK BOX AND WHITE BOX TESTING METHODS
Authors: Maspupah Asri
Published: October 2024

Software testing methods play a crucial role in ensuring the quality, security, and performance of applications. The two main approaches often used are black box and white box testing. This article presents a comprehensive literature review of the characteristics, advantages and disadvantages of both testing methods with the aim of providing in-depth insights for practitioners and researchers in the field of software engineering. The research method used is Systematic Literature Review (SLR), which is a technique of collecting information through the process of searching for literature based on research questions, filtering search results, evaluating, and analyzing relevant research from various academic database sources to gain a comprehensive understanding. Through the analysis of various literature studies, the results of this research are the characteristics of each testing method that is more effectively used in best practice to achieve efficient testing with the coverage of literature review sources in the range of 2020 to 2024. The review of these characteristics is based on seven criteria, namely: test focus, test base, tester's point of view, test case design, program error identification method, program code knowledge, and test effectiveness. In addition, this article presents a comparison of the two methods so that the differences in testing methods can be clearly recognised in terms of how they are implemented. Based on the results of review literature, both methods are better used together in order to ensure that the functional and internal code structure of the software is functioning properly.
KONSEP ILMU SOSIAL PROFETIK DAN RELEVANSINYA TERHADAP PENDIDIKAN AGAMA ISLAM TELAAH PEMIKIRAN KUNTOWIJOYO
Authors: Widya pratama Adellia , Mulyadi Acep
Published: October 2024

Abstract: The study aims to determine how Kuntowijoyo thinks about the concept of prophetic social science and its relevance to Islamic religious education. The type of research used is Library Research, where the data sources are generated from various literature from books, articles or scientific papers written by Kuntowijoyo, and others that are related to a problem to be discussed. The results of the study indicate that prophetic social science has three main values, namely: (1) Humanization related to the formation of attitudes, morals and personality of a person in order to reduce acts of violence (2) Liberation, liberation to be able to open the horizons of thought of students (3) Transcendence, will lead to the ideals of prophetic ethics desired by Kuntowijoyo. Returning human nature to God through Islamic religious education with a monotheistic approach in order to become a stronger and more responsible person, where the three values ​​are relevant to Islamic religious education.
PENDIDIKAN ANTI KORUPSI DALAM AL-QURAN (Perskripsi Moral-Religius dalam Memerangi Praktik Rasuah)
Authors: Yuwanamu Irham , Haq Sansan Ziaul
Published: October 2024

This study seeks to integrate anti-corruption education with an Islamic perspective. This study uses a thematic-analytical method whose primary source is the Quran, interpreted from various commentaries. The findings show that, in substance, the Qur'an has taught us not to commit corruption. Phrases found in the Qur'an whose meaning is commensurate with corruption are 'eating other people's property unlawfully' (akl amwal al-nas bi-al-batil), 'eating illegally' (akl al-suht), and bribing judges (al-idla' ila-al-hukkam). This study further shows that corruption falls into the category of unlawful taking away other people's rights, while bribery is one of the doors to this unjust act. Therefore, corruption is a grave sin that will have serious consequences hereafter. The findings of this study can further contribute to the development of anti-corruption education from an Islamic perspective.
ISSUES OF IMPROVING THE INSTITUTION OF JUDICIAL FINE
Authors:   North Caucasus Institute for Advanced Studies (branch) of the Krasnodar University of the Ministry of Internal Affairs of Russia, Nalchik, Russia, SHERIEV A.M. , GOLYANDIN N.P. , ZHUKOVA A.V. ,   North Caucasus College of Innovative Technologies, Pyatigorsk, Russia
Published: October 2024

The purpose of this article is to consider issues of improving the institution of a judicial fine in the Russian criminal law. The authors consider the similarity in name with other institutions and the direct connection with the provisions of Article 46 of the Criminal Code of the Russian Federation. The authors present different views on the conceptual apparatus of the problem under consideration and its limitation from similar definitions in the field of civil procedure, and also make proposals to change the name for a clearer differentiation. The prospects for reforming the judicial fine in the context of improving criminal legal mechanisms are discussed. Conclusions: It is proposed to establish restrictions on exemption from criminal liability in connection with the imposition of a court fine and the termination of criminal prosecution. These restrictions are proposed to be extended not only by category of crime, but also by the object of the crime, as well as by the nature and degree of public danger. That is, it is proposed to exclude the possibility of applying a judicial fine for certain crimes provided for by individual articles of the special part of the Criminal Code of the Russian Federation.
The Effect of Implementing 3S (SIND, SINO, SINI)-based Orthopedic Nursing Care Instrument in Improving Nursing Service Quality and Patient Safety in Indonesia Hospital
Authors: Sunadi Apri , Nursalam Nursalam , Sukartini Tintin , Ahsan Ahsan , Kolomboy Fajrillah
Published: October 2024

Along with the increasing of orthopedic disorders incidence, the provision of orthopedic nursing care in Indonesia is also increasing. The Indonesian National Nurses Association (INNA) has published nursing care standards as a reference for nurses in providing nursing care and standardizing nursing care terminologies. Standard-based orthopedic nursing care instruments have been developed, but their effectiveness has not been investigated in improving the quality of nursing services and patient safety. This study aimed to identify the effect of the application of 3S-based Orthopedic Nursing Care (3S-ONC) on the quality of nursing services and patient safety. Descriptive quantitative analysis using a quasi-experimental method with a pretest and posttest with control group design, involving 106 respondents (53 control, 53 intervention), recruited by purposive sampling technique. In the intervention group, nurses provided nursing care by applying the 3S-based Orthopedic Nursing Care (3S-ONC) instrument. The study showed that in both groups, most of the nurses were in the early adult category, dominated by female sex and most of them had professional education level. There were significant differences in the quality of nursing services (p 0.046; α 0.05) and patient safety (p 0.000; α 0.05) between the intervention group and the control group after the application of the 3S-ONC instrument. The 3S-ONC instrument can improve the quality of nursing services and the safety of patients with orthopedic disorders in hospitals.The 3S-ONC instrument can be considered for the development of a paper-based or computer-based nursing care documentation system to further improve the quality of nursing services and patient safety in healthcare facilities, especially in hospital.
Evaluation of the immune response of peripheral blood mononuclear cells cultured on Ti6Al4V-ELI polished or etched surfaces
Authors: Abreu Hugo , Lallukka Mari , Raineri Davide , Leigheb Massimiliano , Ronga Mario , Cappellano Giuseppe , Spriano Silvia , Chiocchetti Annalisa
Published: October 2024

Introduction

While titanium and its alloys exhibit excellent biocompatibility and corrosion resistance, their polished surfaces can hinder fast and effective osseointegration and other biological processes, such as angiogenesis, due to their inert and hydrophobic properties. Despite being commonly used for orthopedic implants, research focuses on developing surface treatments to improve osseointegration, promoting cell adhesion and proliferation, as well as increasing protein adsorption capacity. This study explores a chemical treatment intended for titanium-based implants that enhances tissue integration without compromising the mechanical properties of the Ti6Al4V substrate. However, recognizing that inflammation contributes to nearly half of early implant failures, we assessed the impact of this treatment on T-cell viability, cytokine production, and phenotype.

Methods

Ti6Al4V with extra low interstitial (ELI) content discs were treated with hydrofluoric acid followed by a controlled oxidation step in hydrogen peroxide that creates a complex surface topography with micro- and nano-texture and modifies the chemistry of the surface oxide layer. The acid etched surface contains an abundance of hydroxyl groups, crucial for promoting bone growth and apatite precipitation, while also enabling further functionalization with biomolecules.

Results

While cell viability remained high in both groups, untreated discs triggered an increase in Th2 cells and a decrease of the Th17 subset. Furthermore, peripheral blood mononuclear cells exposed to untreated discs displayed a rise in various pro-inflammatory and anti-inflammatory cytokines compared to the control and treated groups. Conversely, the treated discs showed a similar profile to the control, both in terms of immune cell subset frequencies and cytokine secretion.

Discussion

The dysregulation of the cytokine profile upon contact with untreated Ti6Al4V-ELI discs, namely upregulation of IL-2 could be responsible for the decrease in Th17 frequency, and thus might contribute to implant-associated bacterial infection. Interestingly, the chemical treatment restores the immune response to levels comparable to the control condition, suggesting the treatment’s potential to mitigate inflammation by enhancing biocompatibility.
Cardiac sarcoidosis: diagnosis and management
Authors: Aftab Abdullah , Szeto Stanley , Aftab Zoha , Bokhari Sabahat
Published: October 2024

Non-caseating granulomatous infiltration of the myocardium is the hallmark of cardiac sarcoidosis (CS). CS manifests clinically as conduction disturbance, ventricular arrhythmia, sudden cardiac death and/or heart failure with reduced ejection fraction. Other than confirmation through endomyocardial biopsy, a diagnosis of probable CS can be established by histological evidence of systemic sarcoidosis in addition to characteristic clinical or advanced imaging findings. Cardiac Magnetic Resonance imaging (CMR) and 18F-flurodeoxyglycose positron emission tomography (FDG-PET) are imaging modalities indispensable in the diagnosis and monitoring of CS. FDG-PET is the method of choice for identifying the active inflammatory phase of CS and in the monitoring and modifying of immunosuppressive treatment. CMR is better suited for assessing cardiac morphology and function. Both modalities are more effective in detecting CS when used in combination than either is alone. Management of CS is primarily based upon observational data of low quality due to a paucity of randomized controlled trials. Corticosteroid therapy and/or tiered-immunosuppression are the mainstays of treatment in reducing myocardial inflammation. Steroid-sparing agents aim to limit the unfavorable side-effects of a significant steroid burden. Antiarrhythmics and guideline-directed medical therapies are utilized for control of ventricular arrhythmia and left ventricular dysfunction respectively. CS necessitates multidisciplinary care in specialized centers to most effectively diagnose and manage the disease. Additional randomized trials are warranted to further our understanding of medical optimization in CS.
Complicated atrial myxoma with coro-cameral fistula arising from its feeding branch: a case report
Authors: Li Jian-Qiang , Dong Ping , Wang Qian-Li , Li Xiao-Xia , Sha Tu-Min , Zhang Peng , Zhao Zhen-Qing , Liu Chao-Liang
Published: October 2024

A 56-year-old man with a 5-year history of paroxysmal palpitations, which have worsened over the past year, was diagnosed with atrial fibrillation. During evaluation, transesophageal echocardiography revealed a left atrium (LA) tumoral mass attached to the atrial septal fossa ovale, with intra-tumoral blood flow and blood stream draining from the mass. Both coronary computed tomography angiography and coronary angiography demonstrated a coro-cameral fistula connection between the left circumflex artery (LCX) branch and the LA. In addition, they showed feeding arteries of the mass arising from the LCX. The patient underwent surgical resection of the LA mass and repair of the coronary artery fistula. Intraoperative exploration revealed a 1.7 cm × 1.0 cm jelly-like, brittle LA mass and confirmed a rupture of the supplying artery, leading to a coronary artery–left atrial fistula. Surgical ligation was executed to ensure complete sealing of the supplying coronary branch within the atrial septum. Histopathological examination confirmed the diagnosis of left atrial myxoma. The 6-month follow-up indicated no recurrence of the myxoma and restoration of sinus rhythm after radiofrequency ablation. In the literature, cases of a left circumflex artery branch–left atrial fistula due to rupture of the artery supplying a left atrial myxoma are rare.
Association between air pollutants, thyroid disorders, and thyroid hormone levels: a scoping review of epidemiological evidence
Authors: Yang Kaijie , Zhang Guofeng , Li Yongze
Published: October 2024

Background

Over the past two decades, the incidence of thyroid disorders has been steadily increasing. There is evidence to suggest that air pollution may be one of the etiological factors of thyroid diseases. This comprehensive review aimed to examine the evidence related to air pollutants and thyroid disorders and thyroid hormones levels from an epidemiological perspective.

Methods

The scoping review adopted a systematic approach to search for, identify, and include peer-reviewed articles published in English. We performed a comprehensive search of three databases-PubMed, Embase, and Web of Science to identify relevant literature on the relationship between air pollution [particulate matter, nitrogen oxide, carbon monoxide (CO), ozone (O3), sulfur dioxide (SO2)] exposure and thyroid disorders, including hypothyroidism, congenital hypothyroidism (CH), thyroid nodules, thyroid cancer, autoimmune thyroid diseases, as well as thyroid hormone levels, such as thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), and free thyroxine (FT4). Articles published until August 1, 2023, were included.

Results

A total of 3,373 studies were retrieved, and among them, 25 studies covering eight different air pollutants were relevant. The most frequently studied air pollutants in this review included fine particulate matter (with fine particulate matter (PM2.5), n=21; inhalable particles (PM10), n=10; PM10-2.5, n=1) and nitrogen oxides (with NO2, n=13; NOx, n=3). The thyroid disorders and thyroid hormone levels most commonly associated with evidence of air pollution exposure were hypothyroidism (n=7) and TSH (n=12).

Conclusions

Despite variations in study designs and exposure assessments, the findings consistently highlight the substantial health risks that air pollution, particularly PM2.5, poses to thyroid health, especially among vulnerable populations. Given that our study was limited to epidemiological investigations and the increasing prevalence of toxic substances in the environment, there is an urgent need for further research to elucidate the mechanisms by which these pollutants disrupt thyroid function and contribute to the development of thyroid diseases.
Long term outcomes of pituitary adenomas in Multiple Endocrine Neoplasia type 1: a nationwide study
Authors: Valdés Nuria , Romero Ana , Diego Estrella , Calatayud María , Lamas Cristina , Araujo-Castro Marta , Álvarez-Escolá Cristina , Díaz José A. , Alcázar Victoria , Sastre Julia , Martínez Rosa , Oriola Josep , Paja Miguel , Sánchez-Sobrino Paula , Salinas Isabel , Recio-Córdova José María , Navarro Elena , Chiara María Dolores , Castaño Luis , Casterás Anna
Published: October 2024

Introduction

Historically, Multiple Endocrine Neoplasia type 1 (MEN1)-related pituitary adenomas (PAs) were considered more aggressive and treatment-resistant than sporadic PAs. However, recent studies suggest similarities in their behavior. This study aimed to evaluate the long-term outcomes of MEN1 PAs and identify predictive factors.

Methods

Nationwide multicenter retrospective cohort study of MEN1-related PAs with a minimum 1-year follow-up, collecting patient demographics, germline MEN1 pathogenic variants (PV), PA size, secretory profile, radiological characteristics, treatments, and outcomes.

Results

We analyzed 84 PAs, 69%in females and 31% in males (P<0.001), diagnosed at a mean age of 35.2±14.9 years, mostly through screening (60.7%). Median follow-up was 9 years (IQR:4-16). Prolactin-secreting PAs (PRLomas) (53.5%) and microadenomas (65.5%) were most common. Dopamine agonist treatment was first line for 16 macroPRLomas and 25 microPRLomas, 60.9% of them achieved PRL normalization. There was no significant association observed with tumor size, sex, treatment duration or MEN1 PV. The risk of progression from micro-PA to invasive macro-PA was 7.2% (4/55), after 8 years (IQR:4-13), all of them were microPRLomas. Kaplan-Meier estimation curve showed significantly higher progression probability in microPRLomas than in other microadenomas subtypes (P=0.017) or microNFPAs (P=0.032). No differences were found between sex, age, or germline MEN1 PV.

Conclusion

MEN1-related micro-PAs have a low risk of progressing to invasive macro-PAs, regardless of sex, age at diagnosis, or MEN1 germline PV. The risk is higher for microPRLomas over the long term. Therefore, long-term surveillance with reduced frequency, rather than intensive short-term monitoring, may be appropriate for patients with MEN1-related PAs.
Elevated 12,13-diHOME level in maternal and umbilical cord blood complicated with preeclampsia
Authors: Yoshida Tomohiro , Kitada Kohei , Nakai Kensaku , Uemura Ryo , Kurihara Yasushi , Tahara Mie , Hamuro Akihiro , Nakano Akemi , Misugi Takuya , Tachibana Daisuke
Published: October 2024

Background

Preeclampsia (PE) is a condition in pregnancy characterized by hypertension and proteinuria, thus leading to severe complications for both mother and fetus, including fetal growth restriction (FGR). However, there are still unclear aspects regarding the pathogenesis, prevention, and treatments. This study aimed to elucidate the characteristics of lipid metabolism in maternal and umbilical cord plasma complicated with PE using liquid chromatography-mass spectrometry (LC-MS).

Method

The study included singleton pregnant women at Osaka Metropolitan University Hospital from March 2023 to February 2024. PE was diagnosed based on new-onset hypertension after 20 weeks of gestation and other symptoms such as proteinuria and organ dysfunction. FGR was defined by ultrasound measurements below -1.5 standard deviation (SD). Plasma samples were collected from maternal and umbilical cord blood within 24 hours before delivery. Lipid metabolites were comprehensively analyzed using LC-MS, and the lipokine 12,13-diHOME, identified as elevated in the comprehensive analysis, was quantified. Immunohistochemistry was conducted on placental samples to assess soluble epoxide hydrolase (sEH) expression.

Results

The study involved 31 participants, with 20 in the control group and 11 in the PE group. A comprehensive analysis of maternal plasma samples identified a significant increase in 12,13-diHOME levels in the PE group compared to the control group. Quantification of 12,13-diHOME showed a significant increase in maternal plasma, umbilical venous plasma, and umbilical arterial plasma in the PE group compared to the control group (p = 0.007, p = 0.008, p = 0.005). PE with FGR showed significantly higher 12,13-diHOME concentrations in the umbilical arterial/venous ratio compared to the PE without FGR group (p = 0.03). Negative correlations were observed between 12,13-diHOME levels and birth weight in the PE group. Immunohistochemistry did not show significant differences in the sEH expression between the groups.

Conclusion

This study demonstrated that 12,13-diHOME levels were significantly elevated in maternal and umbilical cord blood in PE patients, particularly in PE with FGR. Elevated 12,13-diHOME may reflect the progression of placental ischemia due to PE pathogenesis. This lipid metabolite could serve as a marker for the severity of preeclampsia, thus providing new insights into perinatal lipidomics and the potential role of 12,13-diHOME in PE.
Exploring the heterogeneity of hepatic and pancreatic fat deposition in obesity: implications for metabolic health
Authors: Deng Ming , Li Zhen , Chen Shangyu , Wang Huawei , Sun Li , Tang Jun , Luo Liman , Zhang Xiaoxiao , Xu Haibo , Dai Zhe
Published: October 2024

Objective

This retrospective observational study investigates the heterogeneity of hepatic and pancreatic fat deposition and its implications for metabolic health in individuals with obesity.

Methods

A total of 706 patients with obesity underwent an MRI to quantify liver and pancreatic fat. Patients were classified into four groups based on fat deposition: no fat (None), fatty pancreas only (NAFPD), fatty liver only (NAFLD), and both conditions (NAFLD+NAFPD). Biochemical profiles, insulin resistance (Homeostatic Model Assessment for Insulin Resistance, HOMA-IR), and β-cell function were analyzed. A series of multiple linear regressions were used to investigate the independent effects of characteristics on glucose, insulin, and C-peptide at 0h. Another multiple linear regression was performed to evaluate the effects of basic characteristics on average liver fat, mean pancreatic fat, and visceral fat.

Results

The majority (76.63%) exhibited both NAFLD and NAFPD, highlighting the heterogeneity of fat deposition among individuals with obesity. Groups with fatty liver displayed significantly higher fasting glucose, insulin, C-peptide, and HOMA-IR levels than those without fatty liver (P < 0.01). Fatty pancreas alone did not significantly influence these metabolic parameters (P > 0.05). This underscores the greater metabolic impact of hepatic fat compared to pancreatic fat.

Conclusions

The study confirms the complex heterogeneity of fat deposition in obesity, with the fatty liver being a more influential factor in metabolic disturbances than the fatty pancreas. The prevalent co-occurrence of NAFLD and NAFPD in this population underscores the need for targeted management strategies focusing on hepatic fat reduction to mitigate metabolic risk.
Unveiling new chapters in medullary thyroid carcinoma therapy: advances in molecular genetics and targeted treatment strategies
Authors: Huai Jia-Xuan , Wang Fang , Zhang Wen-Hui , Lou Yan , Wang Gao-Xiang , Huang Li-Ji , Sun Jing , Zhou Xi-Qiao
Published: October 2024

Medullary Thyroid Carcinoma (MTC), a neuroendocrine malignancy that arises from the calcitonin-secreting parafollicular C-cells of the thyroid, constitutes a minor yet impactful fraction of thyroid malignancies. Distinguished by its propensity for aggressive growth and a pronounced tendency for metastasis, MTC poses formidable obstacles to the early diagnosis and therapeutic intervention. The molecular genetics of MTC, particularly the role of the RET gene and the RAS gene family, have been extensively studied, offering insights into the pathogenesis of the disease and revealing potential therapeutic targets. This comprehensive review synthesizes the latest advancements in the molecular genetics of MTC, the evolution of precision therapies, and the identification of novel biomarkers. We also discuss the implications of these findings for clinical practice and the future direction of MTC research.
Active power balance control of wind-photovoltaic-storage power system based on transfer learning double deep Q-network approach
Authors: Xiao Jun , Zhao Wen , Li Wei , Zhao Yankai , Li Yongzhi , Ma Xudong , Liu Yuchao
Published: October 2024

Introduction

This study addresses the challenge of active power (AP) balance control in wind-photovoltaic-storage (WPS) power systems, particularly in regions with a high proportion of renewable energy (RE) units. The goal is to effectively manage the AP balance to reduce the output of thermal power generators, thereby improving the overall efficiency and sustainability of WPS systems.

Methods

To achieve this objective, we propose the transfer learning double deep Q-network (TLDDQN) method for controlling the energy storage device within WPS power systems. The TLDDQN method leverages the benefits of transfer learning to quickly adapt to new environments, thereby enhancing the training speed of the double deep Q-network (DDQN) algorithm. Additionally, we introduce an adaptive entropy mechanism integrated with the DDQN algorithm, which is further improved to enhance the training capability of agents.

Results

The proposed TLDDQN algorithm was applied to a regional WPS power system for experimental simulation of AP balance control. The results indicate that the TLDDQN algorithm trains agents more rapidly compared to the standard DDQN algorithm. Furthermore, the AP balance control method based on TLDDQN can more accurately manage the storage device, thereby reducing the output of thermal power generators more effectively than the particle swarm optimization-based method.

Discussion

Overall, the TLDDQN algorithm proposed in this study can provide some insights and theoretical references for research in related fields, especially those requiring decision making.
Leveraging advanced AI algorithms with transformer-infused recurrent neural networks to optimize solar irradiance forecasting
Authors: Naveed M. S. , Hanif M. F. , Metwaly M. , Iqbal I. , Lodhi E. , Liu X. , Mi J.
Published: October 2024

Solar energy (SE) is vital for renewable energy generation, but its natural fluctuations present difficulties in maintaining grid stability and planning. Accurate forecasting of solar irradiance (SI) is essential to address these challenges. The current research presents an innovative forecasting approach named as Transformer-Infused Recurrent Neural Network (TIR) model. This model integrates a Bi-Directional Long Short-Term Memory (BiLSTM) network for encoding and a Gated Recurrent Unit (GRU) network for decoding, incorporating attention mechanisms and positional encoding. This model is proposed to enhance SI forecasting accuracy by effectively utilizing meteorological weather data, handling overfitting, and managing data outliers and data complexity. To evaluate the model’s performance, a comprehensive comparative analysis is conducted, involving five algorithms: Artificial Neural Network (ANN), BiLSTM, GRU, hybrid BiLSTM-GRU, and Transformer models. The findings indicate that employing the TIR model leads to superior accuracy in the analyzed area, achieving R2 value of 0.9983, RMSE of 0.0140, and MAE of 0.0092. This performance surpasses those of the alternative models studied. The integration of BiLSTM and GRU algorithms with the attention mechanism and positional encoding has been optimized to enhance the forecasting of SI. This approach mitigates computational dependencies and minimizes the error terms within the model.
The impact of green trade barriers on China’s photovoltaic products exports to ASEAN
Authors: Chen Shumin
Published: October 2024

In the context of the global push towards a green economy, this research investigates the impact of green trade barriers on the export trade volume of Chinese photovoltaic products to ASEAN from 2010 to 2021. The strong evidence indicates that green trade barriers have a positive effect on improving the export trade volume of Chinese photovoltaic products to ASEAN. This conclusion remains robust through multiple robustness tests, including excluding data from pandemic-affected years and the only developed country Singapore. Moreover, the impact of green trade barriers varies with exchange rate levels and language environments. In countries with higher exchange rates and countries with different official languages, the positive effect of green trade barriers on trade is more significant. In contrast, in countries with the same official language, green trade barriers have a negative effect on improving the trade volume. Additionally, higher levels of renewable energy consumption diminish the beneficial effect of TBT on photovoltaic trade, and CO2 emissions lessen the detrimental effect of TBT on photovoltaic trade. The findings provide valuable insights for policymakers, suggesting that align with international standards, provide differentiated technical assistance and adopt transparent trade rules when formulating green trade barriers to better support the competitiveness and export growth of Chinese photovoltaic products in the international market.
TFE3 and TP53 were novel diagnostic biomarkers related to mitochondrial autophagy in chronic rhinosinusitis with nasal polyps
Authors: Wang Ning , Yuan Ying , Jia Yanjun , Han Yue , Yu Xuemin , Fu Ying , Li Xiao
Published: October 2024

Background

Chronic rhinosinusitis with nasal polyps (CRSwNP) belongs to a subtype of Chronic rhinosinusitis which is a heterogeneous inflammatory condition. It has been reported that mitophagy may provide a new therapeutic option for CRSwNP.

Methods

The GSE136825 (training dataset) and GSE179265 (validation dataset) were scoured from the Gene Expression Omnibus database. The candidate genes related to mitophagy were identified by differential expression analysis. Subsequently, the biomarkers were selected from the machine learning, Receiver Operating Characteristic curves, and expression level verification. A backpropagation (BP) neural network was generated to evaluate the diagnostic ability of biomarkers. In addition, the infiltration abundance of immune cells, potential drugs, and related ear-nose-throat (ENT) diseases were analyzed based on the biomarkers. Finally, qPCR analysis was performed to verify these biomarkers.

Results

A total of 8 candidate genes were identified by overlapping 3,400 differentially expressed genes (DEGs) and 72 mitophagy-related genes Subsequently, TFE3 and TP53 were identified as biomarkers of CRSwNP, and the area under the curves (AUC) of the BP neural network was 0.74, which indicated that the biomarkers had excellent abilities. TFE3 and TP53 were co-enriched in the cancer pathway, cell cycle, endocytosis, etc. What’s more, Macrophage and Immature dendritic cells had significant correlations with biomarkers. The drugs (Doxorubicin, Tetrachlorodibenzodioxin, etc.) and the ear-nose-throat diseases (hearing loss, sensorineural, tinnitus, etc.) related to biomarkers were predicted. Ultimately, qPCR results showed that the expression levels of TFE3 and TP53 in polyp tissue of CRSwNP were increased.

Conclusion

Overall, TFE3 and TP53 could be used as biomarkers or potential therapeutic targets to diagnose and treat CRSwNP.
Identification and validation of an m7G-related lncRNAs signature for predicting prognosis, immune response and therapy landscapes in ovarian cancer
Authors: Li Jixin , Wang Hui , Zhang Siyang , Quan Linru , Zhou Xin
Published: October 2024

Background

Ovarian cancer is the most mortality malignancy in gynecology. N7-methylguanosine (m7G) is one of the most prevalent RNA modifications in the development and progression of cancer. The aim of this study is to investigate the effect of m7G-related lncRNA on ovarian cancer in terms of instruction prognosis and immunotherapy.

Methods

After integrating and processing the RNA expression profiles with the clinical sample information in the TCGA database, we initially screened to the m7G-related lncRNAs by Spearman correlation analysis, and subsequently obtained a prognostic model constructed by five m7G-related lncRNAs with Univariate Cox analysis, LASSO regression analysis, and Multivariate Cox regression analysis, after which we further evaluated and validated the prognostic value of the model using Kaplan-Meier survival analysis, Principal component analysis, Nomogram, and ROC curve. In addition, based on this risk model, we explored the differentially enriched pathways and functions of the high and low risk groups, and characterized the immune cells, immune functions, gene mutations, and drug sensitivity between the two groups.

Results

After a series of rigorous filtering, we finally attained a prognostic risk model consisting of KRT7-AS, USP30-AS1, ZFHX4-AS1, ACAP2-IT1, and TWSG1-DT which is excellent in predicting the prognostic survival of ovarian cancer patients as well as existing as an independent prognostic factor. Moreover, the model has certain relevance in the immune cells and functions between high and low risk groups, and simultaneously, the signature has the role of guiding the option of immunotherapy and chemotherapeutic drugs.

Conclusion

Altogether, our study established a tight connection between m7G-associated lncRNAs and ovarian cancer, with potential that the prognostic patterns contribute to steering the prognosis of ovarian cancer patients, measuring the efficacy of immunotherapeutic approaches, and detecting effective chemotherapeutic agents.
Impact of deceased-donor characteristics on early graft function: outcome of kidney donor pairs accepted for transplantation
Authors: Mahler Christoph F. , Friedl Felix , Nusshag Christian , Speer Claudius , Benning Louise , Göth Daniel , Schaier Matthias , Sommerer Claudia , Mieth Markus , Mehrabi Arianeb , Renders Lutz , Heemann Uwe , Krautter Markus , Schwenger Vedat , Echterdiek Fabian , Zeier Martin , Morath Christian , Kälble Florian
Published: October 2024

Introduction

The impact of deceased donor characteristics on kidney transplant outcomes is controversial. Correspondingly, the predictive performance of deceased donor scores remains moderate, and many transplant centers lack validated criteria for graft acceptance decisions. To better dissect donor-related risk from recipient and periprocedural variables, we analyzed outcomes of kidney donor pairs transplanted in different individuals.

Methods

This study explored (a)symmetry of early outcomes of 328 cadaveric kidney transplant recipients from 164 donor pairs transplanted at three Eurotransplant centers. The primary discriminatory factor was (a)symmetry of partner graft function, defined as early graft loss or impaired graft function [estimated glomerular filtration rate (eGFR) <30 mL/min] 3 months after transplantation. We reasoned that a relevant impact of donor factors would result in a high concordance rate of limited graft function or failure.

Results

The observed number of symmetric graft failure after transplantation was less than statistically expected (3 months: 1 versus 2, p = 0.89; and 12 months: 3 versus 5, p = 0.26). However, we found a trend toward an impaired 5-year graft survival of grafts with good function 3 months after transplantation but a failed or impaired partner graft compared to symmetrically well-functioning grafts (p = 0.09). Subsequently, we explored the impact of individual donor and recipient variables on early transplant outcomes. Generalized estimating equations after feature selection with LassoGEE bootstrap selected donor age, donor body mass index, and donor eGFR as the relevant risk factors.

Discussion

Our findings indicate that donor factors impact early outcomes in kidney transplantation but may have a limited role in long-term graft survival, once a graft has been accepted for transplantation. Utilizing donor-based clinical scores has the potential to aid clinicians in acceptance decisions, giving them an estimate of individual posttransplant outcomes. However, the ultimate decision for acceptance should rest with clinicians, who must consider the complex interplay of donor factors, as well as recipient and periprocedural characteristics.
Using genetics to explore complement C5 as a druggable protein in periodontitis
Authors: Alayash Zoheir , Baumeister Sebastian-Edgar , Holtfreter Birte , Kocher Thomas , Baurecht Hansjörg , Ehmke Benjamin , Reckelkamm Stefan Lars , Nolde Michael
Published: October 2024

Aim

An excessively activated or dysregulated complement system has been proven to be a vital contributor to the pathogenesis of periodontitis. It has been previously hypothesized that inhibiting the activity of complement component C5 by targeting the C5a receptor is a powerful candidate for treating periodontitis. Here, we apply the drug target instrumental variable (IV) approach to investigate the therapeutic effect of genetically proxied inhibition of C5 on periodontitis.

Method

In our primary analysis, we used 26 independent ‘cis’ single nucleotide polymorphisms as IVs from the vicinity of the encoding locus of C5 that are associated with plasma C5 levels. In a secondary analysis, we assess the validity of our primary findings, exploring the involvement of alternative downstream biomarkers, interleukin 17 (IL-17), interleukin 1β (IL-1β), and tumor necrosis factor (TNF). Summary statistics of plasma levels (C5, IL-17, IL-1β, and TNF) were obtained from a genome-wide association study (GWAS) of 35,559 European descent individuals. We extracted association statistics from a GWAS of 17,353 clinical periodontitis cases and 28,210 European controls. Wald ratios were combined using inverse-variance weighted meta-analysis.

Results

In our primary approach, inhibiting C5 reduced the risk of periodontitis (Odds ratio 0.89 per 1 standard deviation reduction in C5; 95% confidence Interval 0.80–0.98, p value=0.022). Our secondary analysis suggests an involvement of IL-17 within the potential causal pathway, but was inconclusive for other biomarkers.

Conclusions

The findings from our study suggest that C5 inhibition may reduce the risk of periodontitis, prioritizing C5 inhibitors as a potential adjunctive therapeutic intervention in this disease.
Case report: Intralesional secukinumab injection for pediatric nail psoriasis: does it have to be a positive outcome?
Authors: Wang Xuesong , Sun Yonghu , Xie Weixing , Liu Hong , Liu Guoyan
Published: October 2024

Recent studies have shown that local injection of secukinumab can achieve positive therapeutic effects when applied in the treatment of nail psoriasis. At present, there have been no other studies on the use of biological agents in the treatment of pediatric nail psoriasis. Three children were included in the study to evaluate the efficacy and safety of periungual injection and long-term injection of secukinumab in the treatment of nail psoriasis in children. It was found that local injection did not achieve a remarkable therapeutic effect. The nail lesions were improved continuously by subcutaneous injection once a month.
Immune cell dynamics and the impact on the efficiency of transvascular antitumor interventional therapies in hepatocellular carcinoma patients
Authors: Sun Yuan-Dong , Zhang Hao , Li Yuan-Min , Zhou Chun-Xiao , Han Jian-Jun
Published: October 2024

Objective

This study investigates the impact of transvascular antitumor interventional therapies on immune cell dynamics and its correlation with disease control and progression-free survival (PFS) in hepatocellular carcinoma (HCC) patients.

Methods

A single-center observational case-control study was conducted with 119 HCC patients. Transvascular antitumor interventional therapy were administered based on patient-specific evaluations. Peripheral blood samples were collected before and within 28 days after the first treatment to analyze lymphocyte subsets and other immune cells.

Results

Higher counts of total white blood cells (WBCs), lymphocytes, monocytes, and basophils were significantly associated with disease control rate. Subgroup analysis revealed that abnormal BMI, diabetes, infection, and multiple lesions were significantly associated with T cell abnormalities. Age, abnormal BMI, hypertension, and abnormal AFP were linked to total T cell abnormalities. NK cells, B cells, Th cells, Tc/Ts cells, and CD4/CD8 ratios did not show significant differences in PFS probabilities.

Conclusion

Higher counts of WBCs, lymphocytes, monocytes, and basophils, play a crucial role in the effectiveness of HCC interventional therapy.
Microbe-immune interactions: new perspectives on coagulation deficiencies, purpura, and other hemorrhagic conditions under the regulation of the gut microbiota
Authors: Ren Ruhua , Huang Xiaohua , Wei Diu , Guo Qing , Wang Chong , Li Mengjie , Yang Lu , Lang Haiyan , Chen Shana
Published: October 2024

Background

The relationship between gut microbiota and coagulation defects, purpura, and other hemorrhagic conditions (CPH) is currently unclear, with causal links yet to be firmly established.

Objective

The causal relationships between gut microbiota and CPH, along with the potential mediating role of immune cells, were studied using Mendelian randomization analysis.

Methods

Data on 412 gut microbiota species, 731 immune cell types, and CPH were methodologically compiled from genome-wide association studies and the FinnGen database. A 2-sample Mendelian randomization approach in 2 stages was used and the causal links between gut microbiota and CPH were statistically analyzed, assessing the potential mediation by immune cells. Sensitivity and reliability were ensured through heterogeneity and pleiotropy tests.

Results

The abundance of Alistipes putredinis (odds ratio [OR]=0.77, 95% confidence interval [CI] 0.64–0.93, P=0.006) was negatively correlated with CPH, whereas the abundance of Bacteroides stercoris (OR=1.25, 95%CI 1.09–1.45, P=0.002) was positively correlated with the risk of CPH. There was no evidence of reverse causality or the potential mediating effects of 731 immune cell types. The abundance of Proteobacteria (OR=0.81, 95%CI 0.71–0.92, P=0.001) and Coprococcus sp. ART55/1 (OR=0.87, 95%CI 0.80–0.96, P=0.005) was negatively associated with the risk of CPH, whereas the abundance of Enterobacteriales/Enterobacteriaceae (OR=1.36, 95%CI 1.12–1.64, P=0.002) was positively correlated with the risk of CPH, with no evidence of reverse causality. Furthermore, CD38 levels on CD3-CD19 cells can serve as a mediating factor for the influence of Proteobacteria on the pathogenesis of CPH, with a mediating effect ratio of 7.26%.

Conclusions

An increase in Proteobacteria abundance leads to a decrease in CD38 expression on CD3-CD19- cells, thereby reducing the risk of developing CPH. CD3 expression on naive CD4+ in mature T cells serves as a mediating factor for the influence of Enterobacteriales/Enterobacteriaceae on the pathogenesis of CPH, whereas IgD CD38br AC expression on B cells serves as a mediating factor for the influence of Coprococcus sp. ART55/1 on the pathogenesis of CPH. The mediating effect is opposite to the overall trend and has a relatively small impact. No significant heterogeneity or pleiotropy was observed.
Role of SARS-CoV-2-specific memory B cells promoting immune protection after booster vaccination in solid organ transplantation
Authors: Donadeu Laura , Gomez-Olles Susana , Casanova Franc , Torija Alba , Lopez-Meseguer Manuel , Boada-Pérez Meritxell , Kervella Delphine , Crespo Elena , Carrera-Muñoz Claudia , Campos-Varela Isabel , Castells Lluís , Cortese Maria F. , Esperalba Juliana , Fernández-Naval Candela , Quintero Jesús , Muñoz Marina , Agüero Fernando , Gonzalez-Costello José , Lladó Laura , Favà Alexandre , Cañas Laura , del Mar de la Hoz-Caballero María , Meneghini Maria , Torres Irina B. , Juvé Mariona , Hafkamp FMJ , Vila Marta , Robles Alba G. , Buzón Maria José , Toapanta Nestor , Zúñiga José Miguel , Monforte Víctor , Saez-Giménez Berta , Len Oscar , Arcos Ibai Los , Miret Enric , Ariceta Gema , Pardo Emma , Martínez Xavier , Moreso Francesc , Bestard Oriol
Published: October 2024

Introduction

Solid organ transplant (SOT) recipients display weak seroconversion and neutralizing antibody (NAb) responses after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination and remain at risk of severe coronavirus disease 2019 (COVID-19). While B-cell memory is the hallmark of serological immunity, its role in driving successful vaccine responses and providing immune protection in SOT patients remains unclear.

Methods

We investigated the function and interplay of SARS-CoV-2-specific memory B cells (mBc), different cytokineproducing T cells, and cross-reactive NAb in driving seroconversion and protection against COVID-19 in two cohorts. First, we studied a large cohort of 148 SOT recipients and 32 immunocompetent individuals who underwent several vaccinations. Subsequently, we assessed 25 SOT patients participating in a randomized controlled trial to compare two different immunosuppressive strategies for allowing successful seroconversion and memory-cell responses after booster vaccination.

Results

We corroborate previous findings that B- and T-cell memory responses are weaker and more delayed in SOT patients than in immunocompetent (IC) individuals; however, within the SOT cohort, we found that these responses are relatively stronger and more robust in patients not receiving mycophenolate mofetil (MMF)-based therapies. Anti- spike IgG titers strongly correlated with RBD-specific IgG-producing mBc, with both displaying broad viral cross reactivity. Prebooster SARS-CoV-2-specific mBc and IL-2- producing T cells accurately predicted Nab seroconversion (AUC, 0.828) and protection against severe COVID-19. While switching unresponsive SOT patients from calcineurin inhibitors (CNI)/MMF to a low-exposure CNI/mTOR-i regimen favored wider SARS-CoV-2-specific immune responses after a fourth booster vaccination, preformed RBD-specific mBc predicted NAb seroconversion.

Discussion

Our study adds new insights into the pathobiology of immune memory and highlights the pivotal role of SARS-CoV-2-specific mBc in promoting immune protection inSOT patients.
BANF1 is a novel prognostic biomarker linked to immune infiltration in head and neck squamous cell carcinoma
Authors: He Yaodong , Li Huan , Li Jing , Huang Junhong , Liu Rong , Yao Yanbing , Hu Yating , Yang Xinjie , Wei Jianhua
Published: October 2024

Background

Barrier-to-autointegration factor 1 (BANF1) is an abundant and ubiquitously expressed postnatal mammalian protein that is overexpressed in numerous human cancers and can promote cancer cell proliferation. However, the role of BANF1 in prognosis remains unclear in head and neck squamous cell carcinoma (HNSCC).

Methods

BANF1 expression data were obtained from the GEO and TCGA databases. We used Cox regression and Kaplan–Meier curves to assess the prognostic potential of BANF1. The role of BANF1-related genes was investigated using Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses. In addition, we explored the link between BANF1, drug sensitivity, and the tumor immune microenvironment. Finally, functional in vitro and in vivo assays were used to explore the effects of BANF1 on tumor growth and metastasis of HNSCC.

Results

BANF1 was markedly overexpressed in HNSCC and was correlated with clinicopathological characteristics. According to survival analysis, BANF1 can be inversely correlated with patient survival and can act as a prognostic risk indicator. IC50 values for chemotherapeutic treatments indicated that the group with high BANF1 expression was more responsive to most antitumor treatments. Furthermore, higher TIDE scores were observed in the low BANF1 expression group, indicating a decline in the efficacy of immune checkpoint inhibitor therapy. Functionally, the malignant biological behavior of HNSCC cell lines was inhibited when BANF1 expression was knocked down.

Conclusion

BANF1 can promote tumor progression in patients with HNSCC. BANF1 shows great promise as a potential biomarker to assess the prognosis.