bluuebunny/crossref_metadata_2025 huggingface dataset:
Motion Control of Autonomous Underwater Vehicle Based on Fractional Calculus Active Disturbance Rejection
Authors: Wan Junhe , Liu Hailin , Yuan Jian , Shen Yue , Zhang Hao , Wang Haoxiang , Zheng Yi
Published: December 2021

An active disturbance rejection control based on fractional calculus is proposed to improve the motion performance and robustness of autonomous underwater vehicle (AUV). The active disturbance rejection control (ADRC) method can estimate and compensate the total disturbance of AUV automatically. The fractional-order PID (proportional integral derivative) has fast dynamic response, which can eliminate the estimation error of extended state observer. The fractional calculus active disturbance rejection strategy combines the advantages of the above two algorithms, and it is designed for AUV heading and pitch subsystems. In addition, the stability of fractional calculus ADRC heading subsystem is proven by Lyapunov stability theorem. The numerical simulations and experimental results document that the superior performance has been achieved. The fractional calculus ADRC strategy has more excellent abilities for disturbance rejection, performs better than ADRC and PID, and has important theoretical and practical value.
Distributed Consensus of USVs under Heterogeneous UAV-USV Multi-Agent Systems Cooperative Control Scheme
Authors: Xue Kai , Wu Tingyi
Published: December 2021

This paper addresses the formation motion control of heterogeneous multi-agent unmanned systems via a distributed consensus approach. The considered heterogeneous system consisted of unmanned aerial vehicles (UAVs) and unmanned surface vehicles (USVs). A leader-following consensus scheme and APF method are used to construct UAV-USVs Formation task requirements. A fuzzy-based sliding mode control approach is proposed to ensure the formation assembles in a finite time, and the finite-time stability is proved by the Lyapunov stability theorem. To highlight the cooperation within the heterogeneous systems, such as UAV and USV, a novel vision-based path re-planning approach is proposed. Simulation results confirm the efficiency of the proposed approach.
The Close Relationship between Internal Wave and Ocean Free Surface Wave
Authors: Chen Bang-Fuh , Huang Yi-Jei
Published: December 2021

A numerical model was used to simulate the propagation of internal waves (IW) along the surface layer. The results show that strong water exchange during IW propagation results in strong free surface flow and produces small but distinct free surface waves. We found a close relationship between the internal and ocean surface waves. Our intuitive reaction is that by training the relationship between the water surface wave height and the internal wave waveform, the internal wave waveform can be reversed from the water surface wave height value. This paper intends to validate our intuition. The artificial neural network (ANN) method was used to train the Fluent simulated results, and then the trained ANN model was used to predict the inner waves below by the free surface wave signal. In addition, two linear internal wave equations (I and II) were derived, one based on the Archimedes principle and the other based on the long wave and Boussinesq approximation. The prediction by equation (II) was superior to the prediction of equation (I), which is independent of depth. The predicted IW of the proposed ANN method was in good agreement with the simulated results, and the predicted quality was much better than the two linear wave formulas. The proposed simple method can help researchers infer the magnitude of IW from the free surface wave signal. In the future, the spatial distribution of IW below the sea surface might be obtained by the proposed method without costly field investigation.
Demographics of Scomberomorus commerson in the Central Taiwan Strait
Authors: Weng Jinn-Shing , Cheng Li-Chi , Lo Yun-Sin , Shiao Jen-Chieh , He Jia-Sin , Lee Ming-An , Liu Kwang-Ming
Published: December 2021

The narrow-barred Spanish mackerel Scomberomorus commerson is an economically essential species; however, few studies have investigated its demographic structure in the northwestern Pacific, which includes Taiwan’s waters. This study examined the growth parameters, age composition, mortality, and sex ratio of S. commerson catches by examining sagittal otoliths and other biological data collected in a 3-year project from June 2018 to June 2021. The transverse sections of sagittal otoliths exhibited alternating translucent and opaque zones, in annual cycles, and this observation was validated by otolith edge analysis. Opaque zones began to form in October; the growth peaked in December and lasted until March. Growth parameters were estimated for female (L∞ = 144.1 cm fork length [FL], k = 0.39 y−1, to = −0.85 y) and male (L∞ = 136.0 cm FL, k = 0.32 y−1, to = −1.49 y) specimens. The maximum recorded FL, body weight, and age were 159.0 cm, 27 kg, and 9.2 y for female and 135.0 cm, 17.8 kg, and 7.2 y for male specimens. Rapid growth was observed for both sexes, with FL reaching 66.8 ± 14.2 cm in female specimens and 70.1 ± 11.0 cm in male specimens during the first year of life. An age–length key based on the direct otolith aging and FL dataset (N = 646) was used to estimate the age composition of 3-year catches measured at landing (N = 16,133). The results verified that the S. commerson currently caught in the central Taiwan Strait are mainly young fish aged 1+ to 2+ y. The estimated fishing mortality (0.27 y−1) and exploitation rate (0.30) suggested that overfishing was not occurring in this stock. The findings of this study have helped clarify the population dynamics of the S. commerson in the Taiwan Strait, and the biological parameters reported herein can aid the management and conservation to ensure the sustainability of this species in this region.
Deriving the 100-Year Total Water Level around the Coast of Corsica by Combining Trivariate Extreme Value Analysis and Coastal Hydrodynamic Models
Authors: Louisor Jessie , Rohmer Jérémy , Bulteau Thomas , Boulahya Faïza , Pedreros Rodrigo , Maspataud Aurélie , Mugica Julie
Published: December 2021

As low-lying coastal areas can be impacted by flooding caused by dynamic components that are dependent on each other (wind, waves, water levels—tide, atmospheric surge, currents), the analysis of the return period of a single component is not representative of the return period of the total water level at the coast. It is important to assess a joint return period of all the components. Based on a semiparametric multivariate extreme value analysis, we determined the joint probabilities that significant wave heights (Hs), wind intensity at 10 m above the ground (U), and still water level (SWL) exceeded jointly imposed thresholds all along the Corsica Island coasts (Mediterranean Sea). We also considered the covariate peak direction (Dp), the peak period (Tp), and the wind direction (Du). Here, we focus on providing extreme scenarios to populate coastal hydrodynamic models, SWAN and SWASH-2DH, in order to compute the 100-year total water level (100y-TWL) all along the coasts. We show how the proposed multivariate extreme value analysis can help to more accurately define low-lying zones potentially exposed to coastal flooding, especially in Corsica where a unique value of 2 m was taken into account in previous studies. The computed 100y-TWL values are between 1 m along the eastern coasts and a maximum of 1.8 m on the western coast. The calculated values are also below the 2.4 m threshold recommended when considering the sea level rise (SLR). This highlights the added value of performing a full integration of extreme offshore conditions, together with their dependence on hydrodynamic simulations for screening out the coastal areas potentially exposed to flooding.
Satellite-Derived Topography and Morphological Evolution around Authie Macrotidal Estuary (France)
Authors: Bagot Philippe , Huybrechts Nicolas , Sergent Philippe
Published: December 2021

The wide spatial and temporal coverage of remotely sensing images is an essential asset to analyze the morphological behaviour of fast-changing coastal environments such as estuarine systems. This paper investigates the reliability of intertidal topography mapping around the Authie Bay, a macrotidal estuarine system located on the northern coast of France. A Satellite-Derived Topography technique is developed by relating the green band reflectance of Sentinel-2 images to rapid variations in topography. This method is well suited to small sedimentary structures of the coastal zone with a 0.30 to 0.35 m height accuracy of the constructed Digital Elevation Model (DEM). For the more complex estuarine configuration, the waterline method was applied and resulted in the construction of DEMs with a height accuracy of 0.35 to 0.38 m. Video animations and records of Authie meander positions along transects are created from Sentinel-2 and Landsat satellite archives (1984–2020). These materials allow to highlight a sedimentation phase at the east side of the spit since 2015. It constrains the main channel towards the eastern bank, thus promoting coastal erosion. The monitoring of a severe erosion phase throughout 2019 shows a 130 m retreat of the coastline. Topographic map differentiation led to the detection of a sedimentation anomaly upstream of the bay, probably linked to this erosion event.
Physical and Numerical Investigations on Wave Run-Up and Dissipation under Breakwater with Fence Revetment
Authors: Zhao Enjin , Mu Lin , Hu Zhaoyang , Wang Xinqiang , Sun Junkai , Zhang Zhiyong
Published: December 2021

Revetment elements and protective facilities on a breakwater can effectively weaken the impact of waves. In order to resist storm surges, there is a plan to build a breakwater on the northern shore of Meizhou Bay in Putian City, China. To better design it, considering different environmental conditions, physical and numerical experiments were carried out to accurately study the effects of the breakwater and its auxiliary structures on wave propagation. In the experiments, the influence of the wave type, initial water depth, and the structure of the fence plate are considered. The wave run-up and dissipation, the wave overtopping volume, and the structure stability are analyzed. The results indicate that the breakwater can effectively resist the wave impact, reduce the wave run-up and overtopping, and protect the rear buildings. In addition, under the same still water depth and significant wave height, the amount of overtopped water under regular waves is larger than that under irregular waves. With the increase of the still water depth and significant wave height, the overtopped water increases, which means that when the storm surge occurs, damage on the breakwater under the high tide level is greater than that under the low tide level. Besides, the fence plate can effectively dissipate energy and reduce the overtopping volume by generating eddy current in the cavity. Considering the stability and the energy dissipation capacity of the fence plate, it is suggested that a gap ratio of 50% is reasonable.
Fatigue Damage Evaluation of Compressor Blade Based on Nonlinear Ultrasonic Nondestructive Testing
Authors: Wang Pengfei , Wang Weiqiang , Zheng Sanlong , Chen Bingbing , Gao Zengliang
Published: December 2021

Nonlinear ultrasonic testing is highly sensitive to micro-defects and can be used to detect hidden damage and defects inside materials. At present, most tests are carried out on specimens, and there are few nonlinear ultrasonic tests for fatigue damage of compressor blades. A vibration fatigue test was carried out on compressor blade steel KMN, and blade specimens with different damage degrees were obtained. Then, the nonlinear coefficients of blade specimens were obtained by nonlinear ultrasonic testing. The results showed that the nonlinear coefficient increased with the increase in the number of fatigue cycles in the early stage of fatigue, and then the nonlinear coefficient decreased. The microstructures were observed by scanning electron microscopy (SEM). It was proven that the nonlinear ultrasonic testing can be used for the detection of micro-cracks in the early stage of fatigue. Through the statistical analysis of the size of the micro-cracks inside the material, the empirical formula of the nonlinear coefficient β and the equivalent crack size were obtained. Combined with the β–S–N three-dimensional model, an evaluation method based on the nonlinear ultrasonic testing for the early fatigue damage of the blade was proposed.
Mycorrhizal Fungal Partners Remain Constant during a Root Lifecycle of Pleione bulbocodioides (Orchidaceae)
Authors: Qin Jiao , Feng Jing-Qiu , Zhang Wei , Zhang Shi-Bao
Published: December 2021

Mycorrhizal mutualisms are vital for orchids through germination to adulthood. Fungal species diversity and community composition vary across seasons and plant development stages and affect plant survival, adaptation, and community maintenance. Knowledge of the temporal turnover of mycorrhizal fungi (OMF) remains poorly understood in the eco-physiologically diverse orchids (especially in epiphytic orchids), although it is important to understand the function and adaptation of mycorrhizae. Some species of Pleione are epiphytic plants with annual roots and may recruit different fungal partners during their root lifecycle. Based on continuous samplings of Pleione bulbocodioides during a whole root lifecycle, we characterized the fungal temporal dynamics using Illumina sequencing of the ITS2 region. Our data showed that the plants of P. bulbocodioides were quickly colonized by OMF at root emergence and had a constant OMF composition throughout one root lifecycle, although the OMF richness declined with root aging after a peak occurrence during root elongation. In contrast, the richness of root-inhabiting fungal endophytes kept increasing with root aging and more drastic turnovers were found in their species compositions. Our findings of OMF temporal turnover contribute to further understanding of mycorrhizal associations and adaptation of Orchidaceae and will benefit orchid resource conservation and utilization.
Tr-milRNA1 Contributes to Lignocellulase Secretion under Heat Stress by Regulating the Lectin-Type Cargo Receptor Gene Trvip36 in Trichoderma guizhouence NJAU 4742
Authors: Li Tuo , Liu Jinding , Wang Qin , Liu Yang , Li Ting , Liu Dongyang , Shen Qirong
Published: December 2021

Background: MicroRNA plays an important role in multifarious biological processes by regulating their corresponding target genes. However, the biological function and regulatory mechanism of fungal microRNA-like RNAs (milRNAs) remain poorly understood. Methods: In this study, combined with deep sequencing and bioinformatics analysis, milRNAs and their targets from Trichoderma guizhouence NJAU 4742 were isolated and identified under solid-state fermentation (SSF) by using rice straw as the sole carbon source at 28 °C and 37 °C, respectively. Results: A critical milRNA, TGA1_S04_31828 (Tr-milRNA1), was highly expressed under heat stress (37 °C) and adaptively regulated lignocellulase secretion. Overexpression of Tr-milRNA1 (OE-Tr-milRNA1) did not affect vegetative growth, but significantly increased lignocellulose utilization under heat stress. Based on the bioinformatics analysis and qPCR validation, a target of Tr-milRNA1 was identified as Trvip36, a lectin-type cargo receptor. The expression of Tr-milRNA1 and Trvip36 showed a divergent trend under SSF when the temperature was increased from 28 °C to 37 °C. In addition, the expression of Trvip36 was suppressed significantly in Tr-milRNA1 overexpression strain (OE-Tr-milRNA1). Compared with the wild type, deletion of Trvip36 (ΔTrvip36) significantly improved the secretion of lignocellulases by reducing the retention of lignocellulases in the ER under heat stress. Conclusions: Tr-milRNA1 from NJAU 4742 improved lignocellulose utilization under heat stress by regulating the expression of the corresponding target gene Trvip36. These findings might open avenues for exploring the mechanism of lignocellulase secretion in filamentous fungi.
Comparative Transcriptomics and Gene Knockout Reveal Virulence Factors of Arthrinium phaeospermum in Bambusa pervariabilis × Dendrocalamopsis grandis
Authors: Fang Xinmei , Yan Peng , Guan Mingmin , Han Shan , Qiao Tianmin , Lin Tiantian , Zhu Tianhui , Li Shujiang
Published: December 2021

Arthrinium phaeospermum can cause branch wilting of Bambusa pervariabilis × Dendrocalamopsis grandis, causing great economic losses and ecological damage. A. phaeospermum was sequenced in sterile deionized water (CK), rice tissue (T1) and B. pervariabilis × D. grandis (T2) fluid by RNA-Seq, and the function of Ctf1β 1 and Ctf1β 2 was verified by gene knockout. There were 424, 471 and 396 differentially expressed genes between the T2 and CK, T2 and T1, and CK and T1 groups, respectively. Thirty DEGs had verified the change in expression by fluorescent quantitative PCR. Twenty-nine DEGs were the same as the expression level in RNA-Seq. In addition, ΔApCtf1β 1 and ΔApCtf1β 2 showed weaker virulence by gene knockout, and the complementary strains Ctf1β 1 and Ctf1β 2 showed the same virulence as the wild-type strains. Relative growth inhibition of ΔApCtf1β 1 and ΔApCtf1β was significantly decreased by 21.4% and 19.2%, respectively, by adding H2O2 compared to the estimates from the wild-type strain and decreased by 25% and 19.4%, respectively, by adding Congo red. The disease index of B. pervariabilis × D. grandis infected by two mutants was significantly lower than that of wild type. This suggested that Ctf1β genes are required for the stress response and virulence of A. phaeospermum.
Proteomics of Two Thermotolerant Isolates of Trichoderma under High-Temperature Stress
Authors: Poosapati Sowmya , Ravulapalli Prasad Durga , Viswanathaswamy Dinesh Kumar , Kannan Monica
Published: December 2021

Several species of the soil borne fungus of the genus Trichoderma are known to be versatile, opportunistic plant symbionts and are the most successful biocontrol agents used in today’s agriculture. To be successful in field conditions, the fungus must endure varying climatic conditions. Studies have indicated that a high atmospheric temperature coupled with low humidity is a major factor in the inconsistent performance of Trichoderma under field conditions. Understanding the molecular modulations associated with Trichoderma that persist and deliver under abiotic stress conditions will aid in exploiting the value of these organisms for such uses. In this study, a comparative proteomic analysis, using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/time-of-flight (MALDI-TOF-TOF) mass spectrometry, was used to identify proteins associated with thermotolerance in two thermotolerant isolates of Trichoderma: T. longibrachiatum 673, TaDOR673 and T. asperellum 7316, TaDOR7316; with 32 differentially expressed proteins being identified. Sequence homology and conserved domains were used to identify these proteins and to assign a probable function to them. The thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition, whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high-temperature conditions by reducing the accumulation of misfolded proteins through an unfolded protein response pathway and autophagy. In addition, there were unique, as well as common, proteins that were differentially expressed in the two isolates studied.
Effects of Secondary Metabolites from Pea on Fusarium Growth and Mycotoxin Biosynthesis
Authors: Perincherry Lakshmipriya , Witaszak Natalia , Urbaniak Monika , Waśkiewicz Agnieszka , Stępień Łukasz
Published: December 2021

Fusarium species present ubiquitously in the environment are capable of infecting a wide range of plant species. They produce several mycotoxins targeted to weaken the host plant. While infecting some resistant plants, the host can alter the expression of toxin-related genes and accumulate no/very low amounts of mycotoxins. The ability of the host plant to modulate the biosynthesis of these toxins is entirely depending on the secondary metabolites produced by the plant, often as a part of systemic acquired resistance (SAR). A major role plays in the family of metabolites called phenyl propanoids, consisting of thousands of natural products, synthesized from the phenylalanine or tyrosine amino acids through a cascade of enzymatic reactions. They are also famous for inhibiting or limiting infection through their antioxidant characteristics. The current study was aimed at identifying the differentially expressed secondary metabolites in resistant (Sokolik) and susceptible (Santana) cultivars of pea (Pisum sativum L.) and understanding their roles in the growth and mycotoxin biosynthesis of two different Fusarium species. Although metabolites such as coumarin, spermidine, p-coumaric acid, isoorientin, and quercetin reduced the growth of the pathogen, a higher level of p-coumaric acid was found to enhance the growth of F. proliferatum strain PEA1. It was also noticeable that the growth of the pathogen did not depend on their ability to produce mycotoxins, as all the metabolites were able to highly inhibit the biosynthesis of fumonisin B1 and beauvericin.
Interactions among Escovopsis, Antagonistic Microfungi Associated with the Fungus-Growing Ant Symbiosis
Authors: Christopher Yuliana , Aguilar Celestino , Gálvez Dumas , Wcislo William T. , Gerardo Nicole M. , Fernández-Marín Hermógenes
Published: December 2021

Fungi in the genus Escovopsis (Ascomycota: Hypocreales) are prevalent associates of the complex symbiosis between fungus-growing ants (Tribe Attini), the ants’ cultivated basidiomycete fungi and a consortium of both beneficial and harmful microbes found within the ants’ garden communities. Some Escovopsis spp. have been shown to attack the ants’ cultivated fungi, and co-infections by multiple Escovopsis spp. are common in gardens in nature. Yet, little is known about how Escovopsis strains impact each other. Since microbe–microbe interactions play a central role in microbial ecology and evolution, we conducted experiments to assay the types of interactions that govern Escovopsis–Escovopsis relationships. We isolated Escovopsis strains from the gardens of 10 attine ant genera representing basal (lower) and derived groups in the attine ant phylogeny. We conducted in vitro experiments to determine the outcome of both intraclonal and interclonal Escovopsis confrontations. When paired with self (intraclonal interactions), Escovopsis isolated from lower attine colonies exhibited antagonistic (inhibitory) responses, while strains isolated from derived attine colonies exhibited neutral or mutualistic interactions, leading to a clear phylogenetic pattern of interaction outcome. Interclonal interactions were more varied, exhibiting less phylogenetic signal. These results can serve as the basis for future studies on the costs and benefits of Escovopsis coinfection, and on the genetic and chemical mechanisms that regulate the compatibility and incompatibility observed here.
Collection and Characterization of Wood Decay Fungal Strains for Developing Pure Mycelium Mats
Authors: Cartabia Marco , Girometta Carolina Elena , Milanese Chiara , Baiguera Rebecca Michela , Buratti Simone , Branciforti Diego Savio , Vadivel Dhanalakshmi , Girella Alessandro , Babbini Stefano , Savino Elena , Dondi Daniele
Published: December 2021

Wood decay fungi (WDF) seem to be particularly suitable for developing myco-materials due to their mycelial texture, ease of cultivation, and lack of sporification. This study focused on a collection of WDF strains that were later used to develop mycelium mats of leather-like materials. Twenty-one WDF strains were chosen based on the color, homogeneity, and consistency of the mycelia. The growth rate of each strain was measured. To improve the consistency and thickness of the mats, an exclusive method (newly patented) was developed. The obtained materials and the corresponding pure mycelia grown in liquid culture were analyzed by both thermogravimetric analysis (TGA) and scanning electron microscopy (SEM) to evaluate the principal components and texture. TGA provided a semi-quantitative indication on the mycelia and mat composition, but it was hardly able to discriminate differences in the production process (liquid culture versus patented method). SEM provided keen insight on the mycelial microstructure as well as that of the mat without considering the composition; however, it was able to determine the hyphae and porosity dimensions. Although not exhaustive, TGA and SEM are complementary methods that can be used to characterize fungal strains based on their desirable features for various applications in bio-based materials. Taking all of the results into account, the Fomitopsis iberica strain seems to be the most suitable for the development of leather-like materials.
Chitin Deacetylase, a Novel Target for the Design of Agricultural Fungicides
Authors: Martínez-Cruz Jesús M. , Polonio Álvaro , Zanni Riccardo , Romero Diego , Gálvez Jorge , Fernández-Ortuño Dolores , Pérez-García Alejandro
Published: December 2021

Fungicide resistance is a serious problem for agriculture. This is particularly apparent in the case of powdery mildew fungi. Therefore, there is an urgent need to develop new agrochemicals. Chitin is a well-known elicitor of plant immunity, and fungal pathogens have evolved strategies to overcome its detection. Among these strategies, chitin deacetylase (CDA) is responsible for modifying immunogenic chitooligomers and hydrolysing the acetamido group in the N-acetylglucosamine units to avoid recognition. In this work, we tested the hypothesis that CDA can be an appropriate target for antifungals using the cucurbit powdery mildew pathogen Podosphaera xanthii. According to our hypothesis, RNAi silencing of PxCDA resulted in a dramatic reduction in fungal growth that was linked to a rapid elicitation of chitin-triggered immunity. Similar results were obtained with treatments with carboxylic acids such as EDTA, a well-known CDA inhibitor. The disease-suppression activity of EDTA was not associated with its chelating activity since other chelating agents did not suppress disease. The binding of EDTA to CDA was confirmed by molecular docking studies. Furthermore, EDTA also suppressed green and grey mould-causing pathogens applied to oranges and strawberries, respectively. Our results conclusively show that CDA is a promising target for control of phytopathogenic fungi and that EDTA could be a starting point for fungicide design.
The Role of Dimorphism Regulating Histidine Kinase (Drk1) in the Pathogenic Fungus Paracoccidioides brasiliensis Cell Wall
Authors: Navarro Marina Valente , de Barros Yasmin Nascimento , Segura Wilson Dias , Chaves Alison Felipe Alencar , Jannuzzi Grasielle Pereira , Ferreira Karen Spadari , Xander Patrícia , Batista Wagner Luiz
Published: December 2021

Dimorphic fungi of the Paracoccidioides genus are the causative agents of paracoccidioidomycosis (PCM), an endemic disease in Latin America with a high incidence in Brazil. This pathogen presents as infective mycelium at 25 °C in the soil, reverting to its pathogenic form when inhaled by the mammalian host (37 °C). Among these dimorphic fungal species, dimorphism regulating histidine kinase (Drk1) plays an essential role in the morphological transition. These kinases are present in bacteria and fungi but absent in mammalian cells and are important virulence and cellular survival regulators. Hence, the purpose of this study was to investigate the role of PbDrk1 in the cell wall modulation of P. brasiliensis. We observed that PbDrk1 participates in fungal resistance to different cell wall-disturbing agents by reducing viability after treatment with iDrk1. To verify the role of PbDRK1 in cell wall morphogenesis, qPCR results showed that samples previously exposed to iDrk1 presented higher expression levels of several genes related to cell wall modulation. One of them was FKS1, a β-glucan synthase that showed a 3.6-fold increase. Furthermore, confocal microscopy analysis and flow cytometry showed higher β-glucan exposure on the cell surface of P. brasiliensis after incubation with iDrk1. Accordingly, through phagocytosis assays, a significantly higher phagocytic index was observed in yeasts treated with iDrk1 than the control group, demonstrating the role of PbDrk1 in cell wall modulation, which then becomes a relevant target to be investigated. In parallel, the immune response profile showed increased levels of proinflammatory cytokines. Finally, our data strongly suggest that PbDrk1 modulates cell wall component expression, among which we can identify β-glucan. Understanding this signalling pathway may be of great value for identifying targets of antifungal molecular activity since HKs are not present in mammals.
From Naturally-Sourced Protease Inhibitors to New Treatments for Fungal Infections
Authors: Gutierrez-Gongora Davier , Geddes-McAlister Jennifer
Published: December 2021

Proteases are involved in a broad range of physiological processes, including host invasion by fungal pathogens, and enzymatic inhibition is a key molecular mechanism controlling proteolytic activity. Importantly, inhibitors from natural or synthetic sources have demonstrated applications in biochemistry, biotechnology, and biomedicine. However, the need to discover new reservoirs of these inhibitory molecules with improved efficacy and target range has been underscored by recent protease characterization related to infection and antimicrobial resistance. In this regard, naturally-sourced inhibitors show promise for application in diverse biological systems due to high stability at physiological conditions and low cytotoxicity. Moreover, natural sources (e.g., plants, invertebrates, and microbes) provide a large reservoir of undiscovered and/or uncharacterized bioactive molecules involved in host defense against predators and pathogens. In this Review, we highlight discoveries of protease inhibitors from environmental sources, propose new opportunities for assessment of antifungal activity, and discuss novel applications to combat biomedically-relevant fungal diseases with in vivo and clinical purpose.
Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives
Authors: Vila-Santa Ana , Mendes Fernão C. , Ferreira Frederico C. , Prather Kristala L. J. , Mira Nuno P.
Published: December 2021

Microbially produced carboxylic acids (CAs) are considered key players in the implementation of more sustainable industrial processes due to their potential to replace a set of oil-derived commodity chemicals. Most CAs are intermediates of microbial central carbon metabolism, and therefore, a biochemical production pathway is described and can be transferred to a host of choice to enable/improve production at an industrial scale. However, for some CAs, the implementation of this approach is difficult, either because they do not occur naturally (as is the case for levulinic acid) or because the described production pathway cannot be easily ported (as it is the case for adipic, muconic or glucaric acids). Synthetic biology has been reshaping the range of molecules that can be produced by microbial cells by setting new-to-nature pathways that leverage on enzyme arrangements not observed in vivo, often in association with the use of substrates that are not enzymes’ natural ones. In this review, we provide an overview of how the establishment of synthetic pathways, assisted by computational tools for metabolic retrobiosynthesis, has been applied to the field of CA production. The translation of these efforts in bridging the gap between the synthesis of CAs and of their more interesting derivatives, often themselves non-naturally occurring molecules, is also reviewed using as case studies the production of methacrylic, methylmethacrylic and poly-lactic acids.
New Applications of Photodynamic Therapy in the Management of Candidiasis
Authors: Rodríguez-Cerdeira Carmen , Martínez-Herrera Erick , Fabbrocini Gabriella , Sanchez-Blanco Beatriz , López-Barcenas Adriana , EL-Samahy May , Juárez-Durán Eder R. , González-Cespón José Luís
Published: December 2021

The most important aetiological agent of opportunistic mycoses worldwide is Candida spp. These yeasts can cause severe infections in the host, which may be fatal. Isolates of Candida albicans occur with greater frequency and variable resistance patterns. Photodynamic therapy (PDT) has been recognised as an alternative treatment to kill pathogenic microorganisms. PDT utilises a photosensitizer, which is activated at a specific wavelength and oxygen concentration. Their reaction yields reactive oxygen species that kill the infectious microorganism. A systematic review of new applications of PDT in the management of candidiasis was performed. Of the 222 studies selected for in-depth screening, 84 were included in this study. All the studies reported the antifungal effectiveness, toxicity and dosimetry of treatment with antimicrobial PDT (aPDT) with different photosensitizers against Candida spp. The manuscripts that are discussed reveal the breadth of the new applications of aPDT against Candida spp., which are resistant to common antifungals. aPDT has superior performance compared to conventional antifungal therapies. With further studies, aPDT should prove valuable in daily clinical practice.
Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species
Authors: Vázquez-Avendaño Rebeca , Rodríguez-Haas José Benjamín , Velázquez-Delgado Hugo , Rosas-Saito Greta Hanako , Hernández-Domínguez Eric Edmundo , Sánchez-Rangel Diana
Published: December 2021

Neofusicoccum parvum belongs to the Botryosphaeriaceae family, which contains endophytes and pathogens of woody plants. In this study, we isolated 11 strains from diseased tissue of Liquidambar styraciflua. Testing with Koch’s postulates—followed by a molecular approach—revealed that N. parvum was the most pathogenic strain. We established an in vitro pathosystem (L. styraciflua foliar tissue–N. parvum) in order to characterize the infection process during the first 16 days. New CysRPs were identified for both organisms using public transcriptomic and genomic databases, while mRNA expression of CysRPs was analyzed by RT-qPCR. The results showed that N. parvum caused disease symptoms after 24 h that intensified over time. Through in silico analysis, 5 CysRPs were identified for each organism, revealing that all of the proteins are potentially secreted and novel, including two of N. parvum proteins containing the CFEM domain. Interestingly, the levels of the CysRPs mRNAs change during the interaction. This study reports N. parvum as a pathogen of L. styraciflua for the first time and highlights the potential involvement of CysRPs in both organisms during this interaction.
Determinants of Financial Performance of Insurance Companies: Empirical Evidence Using Kenyan Data
Authors: Morara Kamanda , Sibindi Athenia Bongani
Published: December 2021

The drivers of financial success of the insurance industry are of interest to several players in any economy including the government; policymakers; policyholders; and investors. In Kenya; there have been relatively few studies on this topic; most of which look at narrow elements that determine insurance companies’ performance. This article sought to explore the components contributing to the financial performance of insurance firms. We employed a sample consisting of 37 general insurers and 16 life insurers for the period running from 2009 to 2018 and utilised panel data methods in order to establish the determinants of financial performance of Kenyan insurers. The pooled OLS; fixed effects and random effects models were estimated with the financial performance measures (proxied by either ROA or ROE) as the dependent variables. The results of the study documented that insurer financial performance and size were positively related. The study also found that insurer financial performance was negatively related to the age variable. The study also unraveled that higher leveraged insurance companies performed better than their lowly geared peers. This article provides broad analyses of the various drivers of financial performance of the insurance industry in Kenya. The findings of this study contribute to the academic literature on the financial performance of the insurance sector in Kenya and Africa as a whole. Furthermore; it gives pointers to the management of insurance companies on the aspects of their business that would need greater attention to drive and sustain superior financial performance.
Potassium (3-Methyl-2-oxido-1,2,5-oxadiazol-4-yl)dinitromethanide
Authors: Zhilin Egor S. , Meerov Dmitry B. , Fershtat Leonid L.
Published: December 2021

Furoxan derivatives enriched with explosophoric functionalities are promising compounds in the preparation of novel energetic materials. Herein, a previously unknown potassium (3-methyl-2-oxido-1,2,5-oxadiazol-4-yl)dinitromethanide (also referred to as potassium 4-dinitromethyl-3-methylfuroxanate) was synthesized via tandem nitration-reduction reactions of an available (furoxanyl)chloroxime. The structure of the synthesized compound was established by elemental analysis, IR, 1H, 13C and 14N NMR spectroscopy. Thermal stability and mechanical sensitivity of the prepared compound toward impact and friction were experimentally determined.
Relationship between the Tensile Properties and Damping Capacity of Fe-22%Mn-12%Cr-4%Co-3%Ni-2%Si Alloys by Fatigue Stress
Authors: Kim Jae-Hwan , Lee Myong-Soo , Kim Jong-Sig
Published: December 2021

The relationship between the tensile properties and damping capacity of fatigue-damaged Fe-22%Mn-12%Cr-4%Co-3%Ni-2%Si alloy under various magnitudes of fatigue stress was investigated. Analytical results show that α′- and ε-martensite were formed due to fatigue stress. The formed α′- and ε-martensite followed a specific orientation and surface relief and intersected with each other. TEM observation and pattern analysis reveal that both α′- and ε-martensites formed on the austenite. As a result of X-ray diffraction, with an increase in fatigue stress, the volume fractions of α′- and ε-martensite were increased, and the increasing rate of the volume fraction of α′-martensite was higher than that of the ε-martensite. As the fatigue stress increased, the tensile strength and damping capacity increased, but the elongation decreased. Besides, as the strength increased and the elongation decreased, the damping capacity decreased. This result is inconsistent with the general tendency for metals but similar to that of alloys undergoing deformation-induced martensite transformation.
Stiffness Modelling and Performance Evaluation of a Soft Cardiac Fixator Flexible Arm with Granular Jamming
Authors: Gao Likun , He Yanlin , Zhu Hangwei , Sun Guangkai , Zhu Lianqing
Published: December 2021

To meet the practical application requirements of cardiac fixation during off-pump coronary artery bypass surgery, a soft cardiac fixator with a flexible arm was previously designed. To enable the soft cardiac fixator to adapt to uncertain external forces, this study evaluates the variable-stiffness performance of the flexible arm. First, the flexible arm was simplified as a soft silicone manipulator measuring 60 mm × 90 mm × 120 mm, which can actuate, soften, or stiffen independently along the length of the arm by combining granular jamming with input pressure. Then, the soft manipulator was modelled as a cantilever beam to analyse its variable-stiffness performance with granular jamming. Next, based on theoretical analysis and calculations, many experiments were conducted to evaluate the variable-stiffness performance of the soft manipulator. The experimental results demonstrated that the variable-stiffness performance is influenced by the flexible arm length, the size of the granules, and the input pressure.
Analysis on Vibration Characteristics and Suppression Strategy of Vehicle Mounted GPR Detection Device for Tunnel Lining
Authors: Lei Yang , Zou Yong , Tian Tian , Qi Falin , Jiang Bo
Published: December 2021

The GPR antenna is installed on the high-speed railway tunnel detection vehicle through the detection device to carry out non-contact nondestructive and rapid detection of the lining condition. Through simulation calculation and field vibration test data analysis, the vibration characteristics of the detection device are obtained. Vibration excitation is applied to GPR antennas to explore the amplitude-frequency sensitivity of echo signal affected by vibration when GPR detects tunnel lining, thus as to provide a basis for mechanism design and vibration control strategy. Through the optimization of the material properties, structural design, and control program of the detection device, the vibration frequency and amplitude of the detection device are optimized, the adaptive adjustment stability of the control system is improved, and the quality of GPR detection data is enhanced.
Investigation on the Transient Characteristics of Self-Priming Pumps with Different Hub Radii
Authors: Chang Hao , Hong Shiming , Wang Chuan , Peng Guangjie , Fan Fengyi , Ye Daoxing
Published: December 2021

Self-priming pumps, important fluid equipment, are widely used in the disaster relief and emergency fields. Meanwhile, the impeller is the only rotational unit of the self-priming pump, which plays an essential part in the power capability of the pump. In this paper, impellers with different hub radii are proposed; by comparing the internal flow characteristics, blade surface load, pressure pulsation characteristics, and radial force distribution of each scheme, the relationship between transient characteristics and hub radius is obtained. The results present that the impeller with a large hub radius can not only weaken the pressure pulsation, blade surface load, and radial force distribution, but also improve the ability of the blade to work on the internal flow field. Finally, the relevant hydraulic experiment is conducted, with the difference between the experiment and calculation below 3%, which ensures the accuracy of the calculation results.
Design of an Embedded Energy Management System for Li–Po Batteries Based on a DCC-EKF Approach for Use in Mobile Robots
Authors: Chellal Arezki Abderrahim , Gonçalves José , Lima José , Pinto Vítor , Megnafi Hicham
Published: December 2021

In mobile robotics, since no requirements have been defined regarding accuracy for Battery Management Systems (BMS), standard approaches such as Open Circuit Voltage (OCV) and Coulomb Counting (CC) are usually applied, mostly due to the fact that employing more complicated estimation algorithms requires higher computing power; thus, the most advanced BMS algorithms reported in the literature are developed and verified by laboratory experiments using PC-based software. The objective of this paper is to describe the design of an autonomous and versatile embedded system based on an 8-bit microcontroller, where a Dual Coulomb Counting Extended Kalman Filter (DCC-EKF) algorithm for State of Charge (SOC) estimation is implemented; the developed prototype meets most of the constraints for BMSs reported in the literature, with an energy efficiency of 94% and an error of SOC accuracy that varies between 2% and 8% based on low-cost components.
Design and Testing of a WAAM Retrofit Kit for Repairing Operations on a Milling Machine
Authors: Campatelli Gianni , Venturini Giuseppe , Grossi Niccolò , Baffa Francesco , Scippa Antonio , Yamazaki Kazuo
Published: December 2021

Repairing, remanufacturing, and refurbishing high value metal components are crucial to move towards a more sustainable economy. Nowadays, repairing operations on high value parts, such as dies, are generally performed using time-consuming manual approaches that rely on the operator’s expertise. The research idea of this paper is to develop a retrofit kit to provide additive capabilities to an existing milling machine, allowing automatic repairing of components thanks to a fast switch between additive and machining operations without a relevant economic investment such the acquisition of a brand-new machine: the final cost of the solution is lower than 10% with respect to the mean cost of a 5-axis milling machine. The additive technology used in this work is Wire Arc Additive Manufacturing (WAAM) that is characterized by a higher deposition rate and a simpler and cost-effective equipment with respect to other techniques (e.g., laser cladding). The design of the system is illustrated in the paper together with the analysis of the results achieved repairing a test case: a die casting mold made of AISI H13 tool steel.
Electropolishing Parametric Optimization of Surface Quality for the Fabrication of a Titanium Microchannel Using the Taguchi Method
Authors: Mahardika Muslim , Setyawan Martin Andre , Sriani Tutik , Miki Norihisa , Prihandana Gunawan Setia
Published: December 2021

Titanium is widely used in biomedical components. As a promising advanced manufacturing process, electropolishing (EP) has advantages in polishing the machined surfaces of material that is hard and difficult to cut. This paper presents the fabrication of a titanium microchannel using the EP process. The Taguchi method was adopted to determine the optimal process parameters by which to obtain high surface quality using an L9 orthogonal array. The Pareto analysis of variance was utilized to analyze the three machining process parameters: applied voltage, concentration of ethanol in an electrolyte solution, and machining gap. In vitro experiments were conducted to investigate the fouling effect of blood on the microchannel. The result shows that an applied voltage of 20 V, an ethanol concentration of 20 vol.%, and a machining gap of 10 mm are the optimum machining parameters by which to enhance the surface quality of a titanium microchannel. Under the optimized machining parameters, the surface quality improved from 1.46 to 0.22 μm. Moreover, the adhesion of blood on the surface during the fouling experiment was significantly decreased, thus confirming the effectiveness of the proposed method.
Iterative Algorithm for Parameterization of Two-Region Piecewise Uniform Quantizer for the Laplacian Source
Authors: Nikolić Jelena , Aleksić Danijela , Perić Zoran , Dinčić Milan
Published: December 2021

Motivated by the fact that uniform quantization is not suitable for signals having non-uniform probability density functions (pdfs), as the Laplacian pdf is, in this paper we have divided the support region of the quantizer into two disjunctive regions and utilized the simplest uniform quantization with equal bit-rates within both regions. In particular, we assumed a narrow central granular region (CGR) covering the peak of the Laplacian pdf and a wider peripheral granular region (PGR) where the pdf is predominantly tailed. We performed optimization of the widths of CGR and PGR via distortion optimization per border–clipping threshold scaling ratio which resulted in an iterative formula enabling the parametrization of our piecewise uniform quantizer (PWUQ). For medium and high bit-rates, we demonstrated the convenience of our PWUQ over the uniform quantizer, paying special attention to the case where 99.99% of the signal amplitudes belong to the support region or clipping region. We believe that the resulting formulas for PWUQ design and performance assessment are greatly beneficial in neural networks where weights and activations are typically modelled by the Laplacian distribution, and where uniform quantization is commonly used to decrease memory footprint.