bluuebunny/crossref_metadata_2025 huggingface dataset:
Catastrophic Consequences of Spain’s Language Policy against the Background of Russia’s Language Policy
Authors: Guzman Tirado Rafael
Published: March 2023

Introduction. The problem of preserving and protecting the native language today is one of the main ones facing any people thinking about their future in the conditions of globalization, European economic integration and a modern multinational and multi-confessional state. The purpose of this article is to examine the internal processes of the destruction of the status of a single official language as a link between the country and the analysis of the discriminatory status of the Spanish language in modern Spain and the prediction of the consequences of such discrimination of language policy, as well as a comparative analysis of the language situation in Spain and Russia.
Materials and Methods. This section specifies what has served as material for the analysis and describes the research techniques used. The object of the study is the Spanish language as the official language of the Kingdom of Spain, its role and its changes in the regions in the process of political processes over the past decades. Comparative- historical and typological research methods are used, as well as methods of observation, analysis, modeling.
Results. This is the main section, which aims to confirm or reject a working hypothesis (or hypotheses) by means of analysis, synthesis and elucidation of data. The reasons for the unbalanced state of the language situation in Spain, as well as the negative consequences of the language policy pursued by the government, are analyzed. Practical recommendations have been developed to overcome the existing critical problems in the field of the country's language policy, based on the Russian example.
Discussion and Conclusion. In this part, the results are to be briefly summarized and the main directions for further research indicated. The results presented in the article contribute to the scientific understanding of the problem of the status of the state official language in modern Spain. They contribute to the development of awareness of the seriousness and danger of the problem. If we Spaniards do not realize the seriousness of what is happening and do not force the government, the opposition and all legitimate parties to respond to this critical situation, then the future of the Spanish language and the Spaniards themselves in Spain seems pessimistic, since we will be foreigners in our beautiful country.
On a class of self-affine sets on the plane given by six homotheties
Authors: Bagaev Andrey V.
Published: March 2023

This paper is devoted to a class of self-affine sets on the plane determined by six homotheties. Centers of these homotheties are located at the vertices of a regular hexagon P
, and the homothetic coefficients belong to the interval (0,1)
. One must note that equality of homothetic coefficients is not assumed. A self-affine set on the plane is a non-empty compact subset that is invariant with respect to the considered family of homotheties. The existence and uniqueness of such a set is provided by Hutchinson's theorem. The goal of present work is to investigate the influence of homothetic coefficients on the properties of a self-affine set. To describe the set, barycentric coordinates on the plane are introduced. The conditions are found under which the self-affine set is: a) the hexagon P
; b) a Cantor set in the hexagon P
. The Minkowski and the Hausdorff dimensions of the indicated sets are calculated. The conditions providing vanishing Lebesgue measure of self-affine set are obtained. Examples of self-affine sets from the considered class are presented.
REPORTE DE CASO: BACTERIEMIA POR EDWARDSIELLA TARDA EN UNA PACIENTE CON NEUROFIBROMATOSIS Y SARCOMA FUSOCELULAR DE ALTO GRADO
Authors: Restrepo Marín Juan David , Fernández Chica Dinno Alberto , Ospino Guzmán Jéssica Liliana , Sánchez Garcés Juan Pablo , Valiente Meléndez Ricardo Jesús
Published: March 2023

La Edwardsiella tarda se ha asociado principalmente a infecciones gastrointestinales, cuando se entra en contacto o se consumen animales acuáticos mal cocidos, incluidos reptiles. Por lo que se ha considerado a E. tarda como un germen que comparte los patrones clínicos de la salmonelosis. Cuando se presenta en huéspedes con algún espectro de susceptibilidad debido a inmunosupresión hasta el 20% del compromiso se ha reportado extraintestinal incluyendo su presentación más severa en forma de bacteriemia.  Presentamos un caso de infección por Edwardsiella tarda en una paciente con antecedente de neurofibromatosis y sarcoma fusocelular de alto grado, bajo tratamiento inmunosupresor, la cual debuto con sangrado genital y rápido deterioro del estado general. Se destaca el papel que presenta la rápida identificación bacteriana por parte del laboratorio clínico usando métodos moleculares y cultivos convencionales para disminuir la incertidumbre diagnóstica, lo que representó un papel crucial en el tratamiento antibiótico final de la paciente y la resolución del proceso infeccioso.

Palabras clave: Bacteriemia, Edwardsiella tarda, Choque Hipovolémico, Inmunosupresión. Fuente:DeCS.
The effectiveness parameters analysis for piers scour calculation
Authors: AL-Jubouri Muhanad , Ray Richard P.
Published: March 2023

A computational fluid dynamics numerical model addressed the problem of local scouring and deposition calculation for non-cohesive sediment and clear water conditions near single and double cylindrical piers. The numerical results of single cylindrical piers correlate very well with the physical model's results while are higher than the case of the double pier, especially when the large-eddy turbulence model, the van Rijn bed-load transport equation, and fine mesh size are considered. Additionally, the final numerical predictions are compared to experimental data after parameters effectiveness explores the range of results based on projected user inputs like the bed-load equation, mesh cell size, and turbulence model.
Complications of pregnancy and childbirth related to sleep disorders
Authors:   Shupyk National Healthcare University of Ukraine, Kyiv, Golianovskiy O.V. , Frolov S.V. ,   Shupyk National Healthcare University of Ukraine, Kyiv
Published: March 2023

Purpose - to conduct a retrospective analysis of complications during pregnancy and childbirth in women with sleep disorders according to medical records. Materials and methods. On the clinical bases of the Department of Obstetrics and Gynecology No. 1 of the Shupyk National Healthcare University of Ukraine during 2020-2022, a retrospective analysis of the course of pregnancy and childbirth was carried out according to the data of medical documentation (individual card of the pregnant/puerperal woman and a questionnaire, history of pregnancy and childbirth) with a total number of 10,694 women. After the selection of two groups of pregnant women according to inclusion and exclusion criteria, the main group consisted of 1,675 pregnant women with signs of sleep disorders, the control group - 7,125 patients with no signs of sleep disorders. The data of the history of pregnancy and childbirth were analyzed according to the most significant risk factors, which were more often found in pregnant women with sleep disorders (maternal age >35 years, body mass index >40 kg/m2), which required the use of sleeping pills. The frequency of complications during pregnancy and childbirth in women with sleep disorders (pregnant anemia, premature birth, development of preeclampsia, delivery by cesarean section) was also analyzed. To evaluate the results of the study, we used the method of statistical testing of hypotheses according to the Student's t-test with determination of the odds ratio and 95% confidence interval (OR with 95.0% CI). Results. We proved that in pregnant women with sleep disorders, the overall frequency of pregnancy complications and abdominal delivery was significantly higher than in pregnant women without sleep disorders (p<0.05). The older age of women with first pregnancy over 35 years and the body mass index of pregnant women >40 kg/m2 influenced sleep disturbances in pregnant women (p<0.05). Conclusions. Sleep disorders are common during pregnancy and pose a real threat to the development of complications during pregnancy and childbirth. Obstetricians and gynecologists and general practitioners who manage pregnancy need to receive information about the quality of sleep of the expectant mother and, in the presence of insomnia, correct the detected disorders in a timely manner with the consultation of specialized specialists. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
The effect of the reparative action drug in treatment of pediatric duodenal ulcer
Authors:   National Pirogov Memorial Medical University, Vinnitsya, Ukraine, Dudnyk V.M. , Buhlova N.O. ,   National Pirogov Memorial Medical University, Vinnitsya, Ukraine, Morozova I.V. ,   National Pirogov Memorial Medical University, Vinnitsya, Ukraine, Kukuruza T.Y. ,   National Pirogov Memorial Medical University, Vinnitsya, Ukraine
Published: March 2023

Background. Digestive tract diseases occupy one of the first places in the structure of childhood pathology, including duodenal ulcers (DU), the development of which in 70-100% of cases is associated with H.pylori infection. It is found that the important role in the development of inflammatory process in digestive tract have toll-like receptors 4 (TLR4), due to reactiveness to H.pylori. It is proved that growth factors, in particular epidermal growth factor (EGF), are able to influence on the healing processes in the mucous membrane. It is advisable to include drugs of reparative action in complex treatment of pediatric DU. Purpose - to evaluate the effect of the reparative action drug in the treatment of pediatric DU, taking into account the dynamics of TLR4 and EGF in blood serum. Materials and methods. The total clinical group consisted of 39 children aged 7-18 years with H.pylori-associated duodenal ulcer. In 20 patients of the group 1 with DU a standard triple regimen was used. In 19 patients of the group 2 a drug of reparative action was included. The content of TLR4 and EGF in serum were determined by ELISA. Results. It was found that the level of TLR 4 decreased by 15.1% and 27.5% after a course of treatment in patients of the group 1 and in the group 2, respectively сompared to a level before treatment. It is showed that in patients of the group 1 and the group 2 after 3 weeks from the start of treatment there were changes in the content of EGF manifested by a decrease by 19.81% and 23.3%, сompared to a level before treatment. Сonclusions. Our results indicated that after the course of treatment the content of TLR4 and EGF in the group 1 of patients was lower by 12.37% and 3.49% respectively, than in the group 2. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.
Noise Immunity of Single-Sideband Modulation Signals with a Controlled Carrier Level
Authors: Dvornikov Sergey , Dvornikov Sergey , Zheglov Kirill
Published: March 2023

Single-sideband modulation is actively used in the organization of communication through the ionospheric channel in the decameter range of radio waves. This is due to the fact that transmissions with single-sideband modulation make it possible to minimize the frequency band while maintaining the information transfer rate and at the same time increase the noise immunity of reception in relation to transmissions with amplitude and frequency analog modulation. At the same time, the widespread use of quadrature synthesis technologies has opened up new possibilities for the formation of transmissions with single-sideband modulation without the direct use of filtering procedures. An analysis of the implementation features of the method of quadrature synthesis of signals with single-sideband modulation showed that the introduction of an additional parameter into its procedures will allow you to control the residual level of the carrier wave, and thereby control the noise immunity of the reception. The opened opportunities made it possible to develop a method and a device for generating a single-sideband modulation signal with an adjustable level of the carrier wave that implements it. The technologies of quadrature synthesis of signals of amplitude modulation and single-sideband modulation with the suppressed carrier are considered both at the level of analytical modeling and using a standard quadrature modulator. The necessity of transition to the analytical form of representation of the modulating signal is substantiated. The role and place of the Hilbert converter in the formation of signals with single-sideband modulation are shown. Known technologies for generating single-sideband modulation signals with a stored pilot signal are considered. The possibility of controlling the value of the stored pilot signal at the level of quadrature synthesis procedures is substantiated. An analytical model and, based on it, a structural diagram have been developed that allow one to generate single-sideband modulation signals with an adjustable pilot signal level. The results of analytical modeling are demonstrated. The value of the provided energy gain as a result of regulation by the residual level of the carrier wave is calculated. Approaches to assessing the noise immunity of transmissions with single-sideband modulation are analyzed. An approach is proposed for calculating the bit error probability of SSB transmissions manipulated by discrete oscillations based on the results of energy redistribution between the carrier oscillation and the sideband, determined by the residual pilot signal level. Conclusions and proposals for the practical implementation of the results obtained are formulated.
Building a Chatbot System to Analyze Opinions of English Comments
Authors: Nguyen Hung , Tan Nguyen , Quan Nguyen , Huong Truong , Phat Nguyen
Published: March 2023

Chatbot research has advanced significantly over the years. Enterprises have been investigating how to improve these tools’ performance, adoption, and implementation to communicate with customers or internal teams through social media. Besides, businesses also want to pay attention to quality reviews from customers via social networks about products available in the market. From there, please select a new method to improve the service quality of their products and then send it to publishing agencies to publish based on the needs and evaluation of society. Although there have been numerous recent studies, not all of them address the issue of opinion evaluation on the chatbot system. The primary goal of this paper’s research is to evaluate human comments in English via the chatbot system. The system’s documents are preprocessed and opinion-matched to provide opinion judgments based on English comments. Based on practical needs and social conditions, this methodology aims to evolve chatbot content based on user inter-actions, allowing for a cyclic and human-supervised process with the following steps to evaluate comments in English. First, we preprocess the input data by collecting social media comments, and then our system parses those comments according to the rating views for each topic covered. Finally, our system will give a rating and comment result for each comment entered into the system. Experiments show that our method can improve accuracy better than the referenced methods by 78.53%.
Performance Analysis of Concatenated Coding to Increase the Endurance of Multilevel NAND Flash Memory
Authors: Trofimov Andrey , Taubin Feliks
Published: March 2023

The increasing storage density of modern NAND flash memory chips, achieved both due to scaling down the cell size, and due to the increasing number of used cell states, leads to a decrease in data storage reliability, namely, error probability, endurance (number of P/E cycling) and retention time. Error correction codes are often used to improve the reliability of data storage in multilevel flash memory. The effectiveness of using error correction codes is largely determined by the model accuracy that exhibits the basic processes associated with writing and reading data. The paper describes the main sources of disturbances for a flash cell that affect the threshold voltage of the cell in NAND flash memory, and represents an explicit form of the threshold voltage distribution. As an approximation of the obtained threshold voltage distribution, a Normal-Laplace mixture model was shown to be a good fit in multilevel flash memories for a large number of rewriting cycles. For this model, a performance analysis of the concatenated coding scheme with an outer Reed-Solomon code and an inner multilevel code consisting of binary component codes is carried out. The performed analysis makes it possible to obtain tradeoffs between the error probability, storage density, and the number of P/E cycling. The resulting tradeoffs show that the considered concatenated coding schemes allow, due to a very slight decrease in the storage density, to increase the number of P/E cycling up to 2–2.5 times than their nominal endurance specification while maintaining the required value of the bit error probability.
OCENA STANU KONSTRUKCJI KOMPOZYTOWYCH POPRZEZ UPROSZCZONE BADANIA REZONANSOWE
Authors: Krzymień Wiesław
Published: March 2023

Badania rezonansowe płatowców, a na ich podstawie obliczenia prędkości krytycznej flatteru, są uznaną od wielu lat metodą wyznaczenia bezpiecznej maksymalnej prędkości lotu. Badania rezonansowe (zwane także badaniami modalnym lub GVT) dotyczą wszystkich powierzchni aerodynamicznych (czyli płatów, stateczników, sterów, trymerów itp.), jak i całej konstrukcji w wybranych wariantach masowych i mają na celu wyznaczenie częstotliwości i postaci drgań własnych. Na ich podstawie przeprowadzane są obliczenia prędkości krytycznej flatteru, a następnie wyznaczana jest bezpieczna maksymalna prędkość lotu. Wykonuje się je zwykle na prototypie lotnym (w wersji ostatecznej) i na tej podstawie konstrukcja zostaje dopuszczona do lotu i otrzymuje certyfikat. Badania rezonansowe są prowadzone w obecnej Pracowni Badania Drgań SBŁ-Instytutu Lotnictwa od wielu lat, jednak nigdy nie wykonywano ich na tym samym egzemplarzu (lub typie) po pewnym czasie po raz drugi. W ramach subwencjonowanego projektu przygotowano i wykonano kilka uproszczonych badań rezonansowych wytypowanych szybowców konstrukcji kompozytowej, by na ich podstawie i posiadanych archiwalnych sprawozdań określić zmiany we właściwościach drganiowych tych konstrukcji.W artykule przedstawiono założenia i metodykę uproszczonych badań rezonansowych oraz wybrane wyniki takich badań kilku polskich szybowców.
Application of computer vision in optimal design of assembled buildings
Authors: Ding Ying , Song Kezhi , Liu Xingzong , Jiang Rui , Zhao Hongxia , Yuan Hongxian , Gong Xiubin , Zhang Keyu
Published: March 2023

The shape data of prefabricated building components are closely related to their safety and reliability. To solve the problem of shape energy-saving optimisation, a radial basis function neural network model based on particle swarm optimisation (PSO) considering temperature compensation is studied and designed, and building information modelling technology is introduced as an auxiliary technology for effective management of visual information, which finally realises the energy-saving calculation of building shape dimensions. The results show that the maximum expansion deformation measured by the proposed model appears at the 28th minute, the maximum expansion deformation is 0.11 mm, the error between the model and the actual value is only 0.02 mm and the difference between the monitoring time points is only 3 min. The total energy consumption values of this model are 36.92, 42.15 and 33.58 kWh/m2 less than those of the PSO model for three types of buildings. In terms of the total contribution rate of energy conservation, the former are 0.76, 0.88 and 2.94% higher than the latter, respectively. Therefore, this research has effectively improved monocular machine vision technology. At the same time, the energy-saving model of shape with temperature compensation for innovative design has also been effectively verified.
BK 5101: Erfahrungen aus der allergologischen Praxis
Authors:   Dermatologie, Allergologie, Dermatohistologie, Berufsdermatologie (ABD), SRH-Klinikum Gera, Elsner Peter
Published: March 2023

Auch wenn bei der Berufskrankheit Nr. 5101 („Schwere oder wiederholt rückfällige Hautkrankheiten“) zahlenmäßig irritative Ekzeme im Vordergrund stehen, ist eine allergologische Abklärung regelmäßig erforderlich, zumal irritative Ekzeme Ausschlussdiagnosen sind. Zu der allergologischen Abklärung gehören neben der serologischen Diagnostik Hauttestungen (Epikutan- und Pricktestungen, heute seltener Scratchtests und Provokationstests). Im Sinne eines erfolgreichen Fallmanagements ist dabei nicht nur auf berufliche Allergene, sondern auch auf Allergene in Hautschutzmitteln und in Therapeutika zu achten. Da die Verfügbarkeit kommerzieller Epikutantestsubstanzen zunehmend eingeschränkt ist, kommt der Testung von Eigensubstanzen der Versicherten eine immer größere Bedeutung zu.
Psychische Belastung: Neue GDA-Empfehlungen
Authors:   Bundesanstalt für Arbeitsschutz und Arbeitsmedizin (BAuA), Berlin, Beck David , Taşkan Esin ,   Berufsgenossenschaft Rohstoffe und chemische Industrie (BG RCI), Kompetenz Center Gesundheit im Betrieb, Mainz
Published: March 2023

Im Rahmen des laufenden Arbeitsprogramms „Psyche“ hat die Gemeinsame Deutsche Arbeitsschutzstrategie (GDA) ihre „Empfehlungen zur Berücksichtigung psychischer Belastung in der Gefährdungsbeurteilung“ weiterentwickelt. Die grundlegend überarbeitete Neuauflage konkretisiert Gestaltungsziele zum Schutz vor Gefährdungen durch psychische Belastung unter Berücksichtigung des aktuellen Wissensstandes und bestehender Vorschriften und Regeln des Arbeitsschutzes. Für die betriebliche Praxis steht damit eine verbesserte Handlungshilfe zur Verfügung.
Die neue AMR 13.2: Arbeitsmedizinische Vorsorge bei Belastungen des Muskel-Skelett-Systems
Authors:   Staatliches Gewerbeaufsichtsamt Hannover, Baars Stefan
Published: March 2023

Körperliche Belastungen sind nach wie vor charakteristisch für zahlreiche Arbeitsplätze und stellen eine bedeutsame Ursache für krankheitsbedingte Ausfälle dar. Für wesentlich erhöhte körperliche Belastungen sieht die Verordnung zur arbeitsmedizinischen Vorsorge (ArbMedVV) seit 2013 eine Angebotsvorsorge vor. Konkretisiert wird dieser Vorsorgeanlass in der Arbeitsmedizinischen Regel (AMR) 13.2, die im Februar 2022 in einer grundlegend überarbeiteten Fassung aktualisiert veröffentlicht wurde. Die wesentlichen Inhalte sind Gegenstand dieses Beitrags.
Chamber music examples and performance analysis for the development of musical talent for undergraduate education
Authors: TÜRKİLİ Erman
Published: March 2023

The development of musical talent can be possible with the mentoring of scientists in the field of musical talent. Academics in the field of music have tested the effectiveness of examples, and their analyzes can make important contributions to the development of musical talent. This research has a qualitative character since it was conducted with a document analysis technique based on experience and observation, and it is an examination of the researcher's selection of materials to be used in music teaching, the reasons for this, and analysis. This study provides fifteen chamber works including string duos, trios, quartets, quintets, and sextets suitable for the undergraduate students. The aim of this study is during undergraduate education, the technical development of the student is prioritized in instrumental studies. Secondly, solo repertoire decision is made by teachers for the young student to expand their repertoire. Chamber music education is neglected in comparison to solo repertoire and technical repertoire. Playing together with other students is a very helpful to hear and play simultaneously. Therefore, this list will help students to analyze their chamber music repertoire and understand the technical difficulties as well as publisher info, timing etc.
B-PER Lysis--CHEM 384 v1
Authors: Christensen Ken
Published: March 2023

The Thermo Scientific B-PER Bacterial Protein Extraction Reagent enables mild extraction of proteins from bacteria (E. coli) without the need for mechanical disruption. The reagent may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates. The B-PER Reagent with Enzymes is supplied with lysozyme and DNase I to improve the extraction efficiency of large (> 70 kDa) molecular weight proteins and proteins expressed in inclusion bodies.
Extraction and PCR for animal samples using the Thermofisher Scientific TaqPath COVID-19 Combo Kit v1
Authors: Stenger Brianna
Published: March 2023

To describe the steps and materials needed to perform a multiplex real-time reverse transcriptase PCR test intended for the qualitative detection of nucleic acid from SARS-CoV-2, the causative agent of COVID-19, in animal respiratory swab samples. This document includes the information and procedures to comply with the ThermoFisher Scientific TaqPath™ COVID-19 Combo Kit under the Food and Drug Administration’s (FDA) Emergency Use Authorization (EUA). The TaqPath™ COVID-19 Combo Kit includes a Real Time PCR assay multiplex that contains three primer/probe sets specific to the SARS-CoV-2 and a primer probe set for bacteriophage MS2 as an internal extraction control. Sections of this procedure are taken directly from the TaqPath™ COVID-19 Combo Kit Instructions for Use, Publication MAN0019181, Version G.0. For additional information, please refer to these instructions.
Phosphorus Extraction -Bray Method v1
Authors: not provided maggie.bowman
Published: March 2023

The method estimates the relative bioavailability of inorganic ortho-phosphate (PO4-P) in soils with acid to neutral pH.
Molecular Observatory Network (MONet) v1
Authors: not provided maggie.bowman
Published: March 2023

EMSL is leading the effort to develop a national network of environmental sampling and sensing sites to produce comprehensive molecular-level information on the composition and structure of soil, water, and resident microbial communities required to advance the span and accuracy of multiscale models of Earth systems. Through the Molecular Observation Network (MONet), EMSL will collaborate with a broad range of partners managing an expanding network of selected natural, urban, and managed watershed, coastal, continental, and atmospheric sites, both experimental and observational. To establish MONet for the Biological and Environmental Research user community, research will focus on seven areas. Research focused under current development include: Establishing the supporting field and experimental networks, Advancing methods for model–data exchange and multiscale modeling, Automating organic matter and soil analysis, Developing the next generation of rhizosphere sensors and other field sensors for plants, microbes, nutrients, biomarkers, and aerosols. The data and models generated through MONet will improve prediction of ecosystem function and response to disturbances, supporting the long-term U.S. Department of Energy goal of scientifically informed decision-making regarding the nation’s energy and environmental security and sustainability. For more information please visit:https://www.emsl.pnnl.gov/monet
Molecular Observation Network (MONet) v2
Authors: not provided maggie.bowman
Published: March 2023

EMSL is leading the effort to develop a national network of environmental sampling and sensing sites to produce comprehensive molecular-level information on the composition and structure of soil, water, and resident microbial communities required to advance the span and accuracy of multiscale models of Earth systems. Through the Molecular Observation Network (MONet), EMSL will collaborate with a broad range of partners managing an expanding network of selected natural, urban, and managed watershed, coastal, continental, and atmospheric sites, both experimental and observational. To establish MONet for the Biological and Environmental Research user community, research will focus on seven areas. Research focused under current development include: Establishing the supporting field and experimental networks, Advancing methods for model–data exchange and multiscale modeling, Automating organic matter and soil analysis, Developing the next generation of rhizosphere sensors and other field sensors for plants, microbes, nutrients, biomarkers, and aerosols. The data and models generated through MONet will improve prediction of ecosystem function and response to disturbances, supporting the long-term U.S. Department of Energy goal of scientifically informed decision-making regarding the nation’s energy and environmental security and sustainability. For more information please visit:https://www.emsl.pnnl.gov/monet
Sistema de categorías para el estudio de la eficacia escolar v1
Authors: Ortiz-de-Villate Carla
Published: March 2023

Sistema de categorías para el estudio de la eficacia escolar. Se ha calculado su confiabilidad y concordancia entre investigadores en nuestra investigación con un Kalpha de Krippendorff alto (α=0.807).
DNA Extraction v1
Authors: not provided izabel.stohel@pnnl.gov
Published: March 2023

A list of all protocols used, from Zymo Research with slight modification. Additionally, protocols for measurement of purity and concentration are listed as well. D6010 is used when there are only a handful of samples and we use tubes, with the corresponding clean and concentrate protocol being D4013 . D4302, D4306, D4308 is the protocol used when there are a large number of samples and we are using a plate, and the corresponding clean and concentrate protocol is D4066. For bottoms of cores that have less DNA, protocol D6110 has been effective.
Core Receiving and Splitting v1
Authors: not provided maggie.bowman
Published: March 2023

The method covers how soil cores were received and depth stratified.
Water Extractable Organic Matter (WEOM) v1
Authors: not provided maggie.bowman
Published: March 2023

The method covers the extraction of water extractable organic matter for soil organic matter characterization using FT-ICR-MS, LCMS, and TOC/TN.
DAPI-Based Polyphosphate Estimation with Extraction Sufficiency Validation:A Method for Quantifying Polyphosphate from Microalgae Samples v1
Authors: Hu Ying-Yu
Published: March 2023

The utilization of DAPI-based fluorometric estimation for polyphosphate (polyP) analysis from microalgae has become increasingly prevalent in field samples since its publication by Martin P. et al. This technique involves evaluating the fluorescence of DAPI-stained samples in quartz cuvettes using a spectrofluorometer. To reduce the consumption of reagent, time, and labor while minimizing DAPI photobleaching, we have adapted this method to a 96-well black microtiter plate with a black film-covered lid. Additionally, the calculation method has been modified to account for matrix effects in microplates. Testing the number of treatment rounds necessary to extract all polyP is crucial. However, even when collecting samples from the same field location or cultivation condition, there can be high variability in treatment rounds among replicates, leading to significant background fluorescence and rendering the polyP from the sample undetectable. This challenge is especially prominent when measuring polyP from field samples. Limited sample availability and insufficient polyP extraction, combined with high background fluorescence, make the laborious measurement unpredictable and hinder accurate polyP measurement. This obstacle is a significant hurdle in polyP measurement. In our assay, we overcome the challenge by validating the sufficiency of extraction for each sample and then measuring the polyP values. To conduct the assay, roughly 400 uL RNase, 400 uL DNase, and 700 uL proteinase are required for four samples.
Extraction and ONT MinION Library Preparation of uHMW gDNA v2
Authors: S. Herzog Kaylee
Published: March 2023

This custom protocol optimizes extraction, purification, and Oxford Nanopore Technologies (ONT) MinION library preparation for ultra-high molecular weight genomic DNA (uHMW gDNA) from parasitic nematodes. It can be used effectively with both low-input samples (e.g., a single adult hookworm) and high-input samples (e.g., a chunk of tissue from an Ascaris sp. adult). Protocols on which this workflow is based: Zymo® Quick-DNATM Magbead Plus Kit protcol Oxford Nanopore Technologies® SQK-LSK-109 gDNA Ligation Sequencing protocol Zymo® DNA Clean & ConcentratorTM Magbead Kit
Sequencing SARS-CoV-2 from Animal Samples with NEBNext® ARTIC SARS-CoV-2 Companion Kit v1
Authors: Gautreau Isabel
Published: March 2023

This protocol is adapted for animal samples and is based on the methods for the NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®), NEB #E7660S/L 24/96 reactions. This protocol does not include a cleanup and normalization step for each sample after cDNA synthesis. Performing the cleanup and normalization step creates library pools where the reads for each library are more evenly distributed. Skipping these steps reduces hands on time but may require a longer sequencing run to obtain sufficient coverage for each sample. To obtain more even sample to sample coverage, we recommend normalizing the RNA samples prior to starting the protocol. Changes in this version: Removal of the human primers Combining multiple barcoding reactions (step 15 and 21) Addition of Loading Flongle Flow Cell section Addition of Analysis section
Soil pH 1:1 Soil Water Ratio v1
Authors: not provided maggie.bowman
Published: March 2023

Soil pH can be measured at either a 1:1 or 1:2 soil to water ratio. Because of the use of the log scale, the two ratios typically provide similar results. A soil with high clay or organic matter may require a 1:2 or even 1:5 ratio to allow for enough fluid to make a measurement. A sample that will also have the lime requirement (i.e. Sikora) measured will be measured with a 1:1 ratio when possible. With either soil to water ratio, the soil and water are placed in a tube and shaken for 15 minutes on a reciprocating shaker to achieve soil solution equilibrium. A calibrated probe accounting for temperature is used for the measurement. One CAL standard sample is included in each batch.
A DIALÉTICA RACIONAL NA RELAÇÃO PRÁTICA EDUCATIVA EM UM AMBIENTE DE INTERVENÇÃO ESCOLAR
Authors: Pereira Santos Luiz Carlos , Santos Eline Alves , Bitencourt Daniela Venceslau , Santana Luzinete Santos
Published: March 2023

É irrefutável o impacto que um projeto pedagógico bem articulado entre docentes e discentes têm na forma como modifica as relações com a instituição. Uma intervenção escolar, quando aplicado em um viés de democracia, assume aqui uma posição proeminente pela forma como se interage e se constroem comunidades escolares prontas para os desafios de mudanças que se esperam. Este texto pretende apresentar uma reflexão na compreensão dialética na relação prática educativa para um ambiente de intervenção escolar. Para tanto, o presente documento busca em teóricos uma resposta para esta relação educativa em um direcionamento da intervenção escolar. Com isso, o tema em análise, para uma prática docente vivenciada para um ambiente de intervenção escolar, emana de uma pesquisa de cunho descritivo, de abordagem qualitativa voltada para auxiliar na compreensão de que os efeitos de uma intervenção compensatória e em tempo útil podem ter consequências muito positivas nas aprendizagens. Quanto ao procedimento bibliográfico, buscou-se um embate entre vários autores da academia na compreensão desta relação.
CURRICULUM ACTS AND NETWORK EDUCATION IN THE COMMUNITY SERVICE AND STRENGTHENING OF BONDS
Authors: Ferreira Simone de Lucena , Nunes Everton de Almeida , Oliveira Bruna Santana de
Published: March 2023

The sociocultural scenarios of the interactions of social actors are forged in the acts of curricula amid the experiences and experiences of the subjects causing ruptures that multi-referenize in the midst of pluralities. These performances show learning processes, presuppose transgressions of the elected formative knowledge and go beyond the walls of the school. This paper aims to present glances at the curricular scenes developed from two Social Assistance Reference Centers (CRAS) located in Aracaju-SE that serve children and adolescents from the Coexistence and Strengthening Service of Bonds (SCFV) using educational practices with digital mobile technologies. From a multi-referential perspective, it is perceived that the methodologies used in CRAS break with the logic of formal teaching, thus opening the possibility of charting a new path in the process of elucidating social phenomena in contemporaneity.
 
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ENSINAR DIVERSIDADE, APRENDER SOLIDARIEDADE
Authors: Garcia Wilton
Published: March 2023

O presente texto articula um posicionamento crítico-reflexivo a respeito de diversidade e solidariedade, em especial atenção às políticas públicas educacionais brasileiras atualmente. Com isso, instaura-se uma pergunta: que tipo de políticas públicas educacionais inscreve diversidade e solidariedade no Brasil? No formato ensaio, o percurso metodológico elege a pesquisa empírica, qualitativa, ao discutir esse questionamento, a partir dos estudos contemporâneos. O resultado desta investigação demonstra dinâmicas estratégicas na educação atual que (re)dimensionam diversidade e solidariedade como potências para a produção de conhecimento, a produção de subjetividade e a produção de informação.
A EDUCAÇÃO PROFISSIONAL E TECNOLÓGICA NO BRASIL: ANOTAÇÕES A PARTIR DOS GOVERNOS DE COLLOR, FERNANDO CARDOSO E LULA
Authors: Araújo José Júlio César do Nascimento , Nicácio Marcondes de Lima , De Oliveira Selma Suely Baçal
Published: March 2023

O artigo discute neoliberalismo e educação profissional nas condições histórico-econômicas da criação dos Institutos Federais (IFs). A análise metodológica centra-se em estudo bibliográfico e documental ancorado no método dialético. Na discussão apresenta-se a educação profissional e tecnológica e as reformas e transformações neoliberais no Brasil marcadas pela interferência de organismos internacionais (1990-2014) que institui-se para o atendimento dos interesses do mercado produtivo em sua mercadologização e expansão articulada às políticas sociais e à oferta ampliada e precarizada. No desfecho, indica que a organização sócio-histórica da educação profissional não se renova, permanecendo espaço de reprodução dos interesses fragmentados e imediatos do capital.
DIREITO À EDUCAÇÃO DAS PESSOAS JOVENS, ADULTAS E IDOSAS EM INTERFACE COM A EDUCAÇÃO ESPECIAL
Authors: Morais Paula Varlanes Brito , Santos José Jackson Reis dos , Lago Danúsia Cardoso
Published: March 2023

A construção histórica do campo da Educação de pessoas jovens, adultas e idosas e da Educação Especial é permeada por avanços, retrocessos e desafios constantes. Nesse sentido, este estudo, de natureza qualitativa, teve por base a pesquisa documental como ferramenta de apoio para compreensão da realidade social, histórica e política e por objetivo analisar o direito assegurado em documentos normativos sobre a educação das pessoas jovens, adultas e idosas em interface com a educação especial. Foram analisados, numa perspectiva histórica e crítica, 11 documentos publicados nos últimos 30 anos, em âmbito nacional e internacional sobre as referidas modalidades educativas, em diálogo com a literatura especializada. A análise demonstra a materialização da (in)visibilidade legal das pessoas com deficiência na interface entre a EPJAI e a Educação Especial na perspectiva da inclusão. As garantias legais estabelecidas surgem de processos, contextos e militâncias de diversos sujeitos que buscam e lutam por espaço, autonomia, cidadania, em meio a tensões e debates, em que as garantias legais passam a ser questionadas também pelos grupos minoritários.
ATIVIDADES EDUCATIVAS EM MUSEUS DE CIÊNCIAS: UMA REVISÃO BIBLIOGRÁFICA
Authors: Giglio Roberta , Sehnem Heck Gabriela , Ferraro José Luís
Published: March 2023

Este artigo tem por objetivo realizar uma revisão bibliográfica acerca da realização de atividades educativas em museus de ciências, buscando subsídios para justificar a relevância desse tipo de atividade na promoção do ensino e da aprendizagem em espaços de educação não formal. A partir de três bases de dados acadêmicos, obtiveram-se 16 trabalhos que se referem a ações educativas vinculadas a setores/programas educativos dentro de espaços de educação não formal, especificamente museus de ciências. Entre eles foram obtidos sete artigos, quatro monografias, três dissertações de mestrado, um capítulo de livro e uma tese de doutorado. A partir da leitura e análise dos trabalhos, emergiram quatro categorias, sendo elas Resultados de atividades educativas realizada em museus de ciências, Desenvolvimento de atividades educativas em museus de ciências, Educação Museal e Processos de aprendizado. Com base nos estudos encontrados, foi possível verificar a relevância das ações educativas nos espaços museais para a construção de conhecimentos dos visitantes no que se refere a uma educação científica.
GÊNERO E SEXUALIDADE NA EDUCAÇÃO INFANTIL: EXPERIÊNCIA FORMATIVA COM PROFESSORAS
Authors: Alencar dos Santos Marta , Dos Santos de Jesus Carla Cristina , Conceição de Santana Miranda Amanaiara
Published: March 2023

Este texto apresenta uma experiência formativa junto as professoras de escolas comunitárias da PenínsulaItapagipana de Salvador/BA no âmbito de um projeto de intervenção, produto do curso de especializaçãoem Gênero e Sexualidade na Educação ofertado pela Universidade Federal da Bahia. Estaformação teve como tema central as discussões sobre gênero e sexualidades na Educação Infantil.Os aportes teóricos utilizados se centram nas discussões sobre os feminismos negros e a interseccionalidade(ASSIS, 2009). Nos encontros com as professoras buscamos refletir com elas as concepçõespessoais e profissionais acerca de gênero e sexualidade em suas vidas a fim de rompermos osestereótipos de gênero e sexualidade presente na sociedade e nas práticas pedagógicas e elegermospedagogias outras que favoreçam o respeito às diferenças.As abordagens metodológicas utilizadas na formação foram orientadas a partir dos estudosde Virgínia Kastrup (1999; 2005) e Amanaiara Conceição Miranda (2018), trazendo uma discussãosobre aprendizagem inventiva, evidenciando os deslocamentos, as desaprendizagens e problematizaçõesnuma ação pedagógica transgressora de um fazer junto as crianças pequenas. Formações deprofessoras como a apresentada neste texto podem contribuir para que as crianças pequenas vivamsuas vidas permeadas pelo afeto e respeito as suas escolhas cotidianas.
O INVÓLUCRO DA NINFA
Authors: Ferraz Santos Gusmão Aline , Thürler Djalma
Published: March 2023

O presente trabalho é resultado de um projeto de intervenção na unidade escolar Colégio EstadualLuís Eduardo Magalhães (CELEM) da cidade de Caraíbas/BA, que pesquisa a performatividade doscorpos femininos no contexto escolar. O trabalho tem como objetivo compreender de que forma épossível observar uma relação de subalternidade de gêneros e um contexto de assédio relacionadosà padronização do fardamento escolar. E tem como premissa a possível associação da vestimenta e daestrutura física dos corpos femininos a um comportamento abusivo de assédio do público masculino.
PRESCRIÇÕES MÉDICAS PARA A VIDA SOCIAL DA MULHER: O CASAMENTO COMO MEDIDA HIGIÊNICA (SÉCULO XIX)
Authors: Alves Araújo de Alencar Juselice , Conceição Joaquim Tavares
Published: March 2023

Este artigo discute prescrições médico-higiênicas para a vida diária de mulheres, tomando como fontes principais teses médicas defendidas na Faculdade de Medicina da Bahia, no século XIX, localizadas na Biblioteca Gonçalo Moniz, da Faculdade de Medicina da Bahia, em Salvador/BA. É recorrente nos assuntos abordados nas teses médicas analisadas o discurso em torno da educação feminina, com diversas temáticas que enfocam regras de higiene para o corpo e prescrições a respeito do comportamento a ser seguido pelas mulheres nos espaços doméstico e público. Dentre essas prescrições, os médicos apontavam o casamento como medida profilática para os males da saúde da mulher e indicavam a idade ideal para as meninas, jovens e mulheres se casarem. A medicina construiu um discurso sobre a educação feminina, considerando o casamento como uma medida preventiva/curativa para as mulheres a fim de evitar determinadas moléstias. Os discursos médico-higiênicos em torno de prescrições higiênicas para o casamento da mulher decorriam da influência da moral católica e do domínio do patriarcalismo e resultou em violência e dominação masculina, com repercussões negativas que podem ser evidenciados no tempo presente.
O QUE OS ALGORITMOS DE BUSCA COMUNICAM SOBRE AS LESBIANIDADES?
Authors: Motter Julianna Paz Japiassu
Published: March 2023

Este trabalho utiliza o evento de mudança dos algoritmos do Google para o termo “lésbica” e associados, em agosto de 2019 para o estudo das plataformas digitais e sua influência e participação nas controvérsias cotidianas relacionadas à sexualidade e gênero, partindo da reflexão de Gillespie (2018) sobre os buscadores como fonte de conhecimento. A pesquisa está metodologicamente e teoricamente alinhada à Teoria Ator-Rede e à Teoria Fundamentada nos Dados. Os resultados das buscas em 18 páginas de resultados do Bing e do Yahoo para o termo "lésbica" apontam para a perspectiva de não neutralidade tecnológica e, com isso para reflexões sobre o papel dos algoritmos na vida cotidiana e nas dinâmicas de poder, podendo criar ou aprofundar dilemas e controvérsias. O objetivo é refletir sobre a persistência de conteúdos pornográficos nessas plataformas e suas consequências. Palavras-chave: lesbianidades; pornografia; algoritmização; plataformização.
INCLUSÃO DIGITAL NO AMAZONAS E O ACESSO DE JOVENS ÀS MÍDIAS SOCIAIS
Authors: Ribeiro Fernandes Wania , De Oliveira Márcio , Machado Melo Fernanda , Fernandes Baptista Jessica , Machado Mendonça Ana Valéria
Published: March 2023

Este artigo decorrente da Pesquisa Nacional em desenvolvimento, intitulada “Comunicação promotora de saúde: estratégias de enfrentamento de epidemias de IST, HIV/AIDS e Hepatites Virais em população jovem”, financiada pelo CNPq no âmbito de contextualização do acesso de jovens nortistas às estratégias de comunicação e informação à saúde. Visa caracterizar a acessibilidade às tecnologias digitais no estado do Amazonas. A metodologia, de cunho bibliográfico descritivo, coletou publicações dos últimos 10 anos sobre o desenvolvimento tecnológico no Amazonas. A análise dos dados descreve o serviço de internet disponível no Estado. Os resultados apontam as especificidades geográficas e econômicas como determinantes para a indisponibilidade de serviços de internet e de acessibilidade. O acesso limitado em municípios do interior, com falhas na transmissão e custo muito elevado, o que reflete no processo de escolarização dos/as jovens e na capacidade de inclusão digital desses sujeitos afetando seu status de visibilidade e importância social.**"Este trabalho contou com financiamento da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) e da Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) e da Universidade Federal do Amazonas (UFAM)".