bluuebunny/crossref_metadata_2025 huggingface dataset:
Allele-specific methylation of the PSA promoter in prostate cells: a new translational marker for the differential diagnosis of prostate cancer
Authors: Baryshev Mikhail , Maksimova Irina , Sasoveca Ilona
Published: September 2023

Background
DNA methylation is one of the mechanisms of epigenetic control of gene expression and a change in the intrinsic pattern can lead to various diseases and disorders. At the same time, this makes DNA methylation a disease-specific biomarker. Here, we show that the acquisition of biallelic methylation status or even biallelic lack of methylation by the PSA promoter is a characteristic feature of cancer cells, while the monoallelic distribution of CpG\CCWGG methylation in the PSA promoter is a hallmark of non-cancerous conditions.
Methods
We performed PCR bisulfite sequencing analysis of the proximal PSA promoter, which has six potential CpG and five CCWGG methylation marks that are targets of DNMTs and therefore have the ability to epigenetically influence gene expression and be a disease-specific biomarker. SNP G-158A, located in the AREI of the PSA promoter, was taken into account when genotyping prostate cell lines for allele-specific methylation analysis. To clarify the differences in PSA promoter methylation we applied RT-qPCR to estimate the level expression of the DNMTs by comparing that with data of gene expression in 54 tissues from GTEx RNA-seq of donors. A PCR cloning sequencing approach and detection of SNPs in exon 3 were used to identify biallelic expression of PSA in the LNCaP cell line.
Results
According to our study of prostate cancer cell lines, the CG/CCWGG methylation in PSA promoter has a great translational ability to be a disease-specific biomarker. Elevated PSA level along with monoallelic promoter methylation would reflect BPH or other non-malignant conditions, whereas a significantly elevated PSA level due to biallelic PSA expression and status of the unmethylated PSA promoter will be consistent with the PCa. The detection of biallelic PSA promoter methylation at the given PSA level will indicate the aggressive PCa. We propose that the CCWGG motif represents an allele-specific methylation signal, which is a novel functionality for this mark involved in the allele-specific methylation mechanism and/or imprinting, as well as its possible role in regulating monoallelic gene expression.
Significance
According to our study in prostate cell line models, the determination of allele-specific methylation in the PSA promoter can be translated into a disease-specific state and can refine PSA-based PCa testing.
Researchers' sex/gender identity influences how sex/gender question is investigated in neuroscience: An example from an OHBM meeting
Authors: Picó-Pérez Maria , Marco Elena Ábalos , Thurston Lindsey , Ambrosi Valerie , Genon Sarah , Bryant Katherine L. , Martínez Ana Belén , Ciccia Lu , Trujillo Anelis Kaiser
Published: September 2023

Gender inequality and diversity in STEM is a challenging field of research. Although the relation between the sex/gender of the researcher and the scientific research practices has been previously examined, less interest has been demonstrated towards the relation between sex/gender of the researcher and the research topic. Here, we examine, from a neurofeminist perspective, both questions: whether sex/gender diversity is related to the examination of sex/gender as a variable and whether sex/gender diversity is related to the topic of study. Using the database of submitted posters to the Organization of Human Brain Mapping 2022 annual conference, we identified abstracts examining a sex/gender-related research question. Among these target abstracts, we identified four analytical categories, varying in their degree of content-related complexity: (1) sex/gender as a covariate, (2) sex/gender as a binary variable for the study of sex/gender differences, (3) sex/gender with additional biological information, and (4) sex/gender with additional social information. Statistical comparisons between sex/gender of researcher and the research topics showed that the proportion of abstracts from Non-binaryor Other first authors compared to both Women and Men was lower for all submitted abstracts than for the target abstracts; that more researchers with sex/gender-identity other than Man implemented analytical category 4; and, for instance, that research involving cognitive, affective, and behavioural neuroscience more frequently fit into categories 4. Word cloud analysis confirmed the validity of the four exploratorily identified analytical categories. We conclude by discussing how raising awareness about contemporary neurofeminist approaches, including perspectives from the global south, is critical to neuroscientific and societal progress.
Amelioration of lithium stress in Vigna radiata (L.) R. Wilczek through the application of Plant Growth Promoting Bacteria
Authors: Hayyat Muhammad Umar. , Nawaz Rab , Siddiq Zafar , Khurshid Iram , Irfan Ali , Mekonnen Amare Bitew , Gaafar Abdel-Rhman Z. , Ahmed Zulkfil , Ali Sikandar , Bourhia Mohammed
Published: September 2023

Lithium is among the alkali metals which can significantly reduce plant growth and yield. In this study, different levels of lithium stress lithium were applied to Vigna radiata (Mung bean), an economically important crop, grown in earthen pots. Each treatment of lithium was compared with the plants inoculated with bacteria that is resistant to lithium. Growth and physiological parameters were measured during the study. The results showed a significant reduction in growth such as plant height, branch number, plant height, root, fresh and dry weight of shoot, seeds quantity per plant, and seeds’ weight, stomatal conductance, photosynthetic and transpiration rate as compared to the controlled and also the plants with Lithium treatment. However, for each treatment, there was a significant increase in the growth and physiological parameters among the plants inoculated with lithium-resistant bacteria. A relationship between lithium concentrations (grains and roots) and the biomass accumulation factor was positive and significant. The lithium level was significantly lower in the roots, shoots, and grains of the plants having bacterial inoculation in comparison with treatments of lithium only. This study showed that lithium can affect the physiological performance and growth of Vigna radiata. This reduction of growth can be significantly minimized by the application of lithium-resistant bacteria. The findings of this research work can be used to understand the lithium effects on the crops and also the utilization of microbes to ameliorate the lithium stress.
Temporal overlapping effect in pump-probe technique on the Rhodamine 6G with addition copper nanoparticle
Authors: khalid Saad , zoory muayyed , Ali Alaa
Published: September 2023

This study took place utilizing the laser "Pump-Probe" probe technique to investigate the properties of a rhodamine 6G laser dye. 532 nm wavelength laser was employed for excitation of the dye "pumping", while another laser with a wavelength of 657 nm was utilized for sensitization "probe". A copper nanomaterial with an average size of 43 nm has been added to the system. The green laser pulse width was modulated at various percentages, including 5%, 10%, 20%, 30%, 40%, and 50% of the original pulse width. The pulse utilized for the pumping process was divided into two components, one of the tow pulse was directed towards the dye, while optical path of the other component was altered by employing multiple mirrors, the modification in the optical path was implemented in order to establish an accurate delay time between the two pulses and control their overlap by extending the duration of the laser pulse. The transmittance laser intensity was determined both before and after the addition of the nanomaterial. Additionally, (FWHM) of the output laser pulse was computed. The wavelength of the output laser pulse was also determined through calculation. The study findings demonstrated a discernible impact resulting from the alteration of laser pulse width as well as the influence of the nanomaterial on the intensity and optical characteristics of the dye. This study is a component of a series of research endeavors focused on comprehending the interplay between nanomaterials and laser dyes as well as the resultant impact of these interactions on the characteristics of laser dyes.
Benefiting from change: range-expanding native thrushes in Argentina
Authors: Vazquez M. Soledad , Scorolli Alberto L. , Zalba Sergio M.
Published: September 2023

The widespread human activity on Earth exerts strong selective pressures on the species with which we coexist. Faced with constant environmental change, species respond with mechanisms of adaptation, expansion, retraction or displacement. Species that establish new populations outside their former range as a result of human-induced environmental change are called 'neonatives'. Many birds now appear to be expanding their ranges in response to habitat modification and climate change. In this study, we use data from three citizen science platforms (eBird, GBIF and VertNet) to document the range expansion of four thrushes in Argentina: Turdus amaurochalinus, T. chiguanco, T. falcklandii, and T. rufiventris. The genus has a history of recent expansion and seems to have benefited from forestation and the landscaping of towns and villages. We found that thrushes have expanded their range about 10-fold since their known distribution in the mid-1980s, expanding at an average linear rate of 35 km/year. In all cases, they also appear to have accelerated their expansion in the last 20 years. Turdus amaurochalinus, T. chiguanco and T. rufiventris have mainly expanded southwards, while T. falcklandii has expanded northwards. This expansion is likely to result in novel ecological interactions, affecting other native species in different ways, and also establishing new relationships with productive activities and urban environments. Thrushes provide an opportunity to understand the response of wild species to human change on the planet, and to design conservation strategies adapted to this new reality.
Application of multi-omics to investigate the effect of Pinus koraiensis cone essential oil on rumen methane emission, microbial community, and metabolites
Authors: Choi Youyoung , Lee Shin Ja , Kim Hyun Sang , Eom Jun Sik , Jo Seong Uk , Guan Le Luo , Lee Sung Sill
Published: September 2023

Background
Enteric methane (CH4) excreted by ruminants is a major source of anthropogenic greenhouse gas emissions in the global environment. Pinus koraiensis cone essential oil (PEO) contains functional compounds such as monoterpene hydrocarbons, which can directly affect the microbiota and their function in the rumen. Previously, we found that PEO oral administration during the growing phases of goats reduced CH4 emissions and was associated with the rumen prokaryotic microbiota. However, a more comprehensive analysis of the rumen microbiota and metabolites are needed. The objective was to elucidate the potential microbial features that underpin CH4 mitigation in goats using metataxonomics (prokaryotes, protozoa, and fungi) and metabolomics (rumen fluid and serum). Ten fattening Korean native goats were divided into two dietary groups: control (CON; basal diet without additives) and PEO (basal diet + 1.5 g/d of PEO), using a 2 × 2 crossover design for 11 weeks. Methane measurements were conducted every four consecutive days for 24–27 d.
Results
Oral administration of PEO reduced CH4 concentrations in the exhaled gas from eructation by 12.0–13.6% (P < 0.05). Although the microbiota structure, including prokaryotes, protozoa, and fungi, was not altered after PEO administration, MaAsLin2 analysis revealed that Selenomonas, Christensenellaceae R-7, and Anaerovibrio were enriched in the PEO group (Q < 0.1). Co-occurrence network analysis revealed that the Bacteroidales RF16 group and Anaerovibrio were the keystone genera in the CON and PEO groups, respectively, with fungal genera exclusively found in the PEO group but not identified as keystone taxa. Predicted function analysis using CowPI, CH4 metabolism was enriched in the CON group, whereas metabolism of sulfur (P < 0.001) and propionate (P < 0.1) were enriched in the PEO group. Random forest analysis identified eight ruminal metabolites, including propionate, that were altered after PEO administration, with predictive accuracy ranging from 0.75 to 0.88. Selenomonas was positively correlated with propionate and co-occurred with it.
Conclusions
The results provide an understanding of how PEO oral administration affects the ruminal microbial community and its functions in the rumen, as well as its linkages with rumen metabolites and host health, ultimately leading to the reduced CH4 emissions.
Pharmacokinetics and Bioequivalence of Two Formulations of Azithromycin Tablets: A Randomized, Single Dose, 3-Period Crossover Study in Healthy Chinese Volunteers Under Fasting and Fed Conditions
Authors: Chen Yingrong , Ye Libing , Mei Jue , Tian Mengli , Xu Min , Jin Qiuyue , Yu Xiang , Yang Shuixin , Wang Jie
Published: September 2023

Background
Azithromycin is the first azalidic antibiotic that is related to the macrolide family of antibiotics. This study was conducted to compare the bioequivalence of two azithromycin tablets under fasting and fed conditions in healthy Chinese volunteers.
Methods
The study was a single-dose, reference-replicated, 3-period crossover trial. A total of 72 healthy subjects (36 subjects in the fasting state study and 36 subjects in the fed study) were enrolled in the study and were randomized to treatment. After the administration of a single oral dose of a 250 mg generic azithromycin tablet (test) or branded azithromycin tablet (reference), blood samples were collected at specific time intervals from 0 to 120 hours. The plasma concentrations of azithromycin were measured by high-performance liquid chromatography–tandem mass spectrometry (HPLC‒MS/MS). Non-compartmental analysis method was employed to determine the pharmacokinetic parameters. Adverse events were also recorded.
Results
In fasting test, the either reference-scaled average bioequivalence (RSABE) approach was used to evaluate the bioequivalence of maximum plasma concentration (Cmax) (withinsubject standard deviation, SWR>0.294), while average bioequivalence (ABE) method was used to evaluate the bioequivalence of area under the cocentration-time curve from time 0 to the time of the last measurable plasma concentration (AUC0 − t ) and area under the cocentration-time curve from time 0 extrapolated to infinity (AUC0−∞) (SWR<0.294). The geometric mean ratio (GMR) of the test/reference for Cmax was 106.49%, and the 95% upper confidence bound was < 0. For AUC0 − t and AUC0−∞ comparisons, GMRs were 103.34% and 101.28%, and the 90% CIs of the test/reference were 95.90%-111.35%, 94.85%-108.15%, respectively. In fed test, the RSABE approach was used to evaluate the bioequivalence of Cmax (SWR>0.294), while ABE approach was used to evaluate the bioequivalence of AUC0 − t and AUC0−∞ (SWR<0.294). The GMRs for Cmax was 99.80%, while the 95% upper confidence bound value was < 0. For AUC0 − t and AUC0−∞ comparisons, GMRs were 97.07% and 98.15%, and the 90% CIs of the test/reference were 90.02%-104.68%, 90.66%-106.25%, respectively. All adverse events were mild and transient.
Conclusions
The trial demonstrated that the test and the reference azithromycin tablets were bioequivalent and well tolerated in Chinese volunteers under fasting and fed conditions.
Trial Registration:
Clinicaltrials, ChiCTR2300071630 (retrospectively registered in 19/05/2023).
The Efficacy of Ocular Lubricants With 0.38% SH Gelling Ingredient and 0.18% SH Non-gelling Ingredient in Treating Dry Eye in Adult Gazan Participants
Authors: Aljarousha Mohammed , Badarudin Noor Ezailina , Azemin Mohd Zulfaezal Che , Aljeesh Yousef , Amer Abuimara , Rahim Muhammad Afzam Shah Abdul
Published: September 2023

Purpose: This study aimed to assess the efficacy of two formulations of lubricant eye drops, containing a gelling agent or not, compared to normal saline.
Study Design: This was a prospective, randomized, double-blinded, three-group, parallel, interventional single-site clinical study.
Methods: Forty-five Gazan participants with moderate to severe dry eye disease (DED) were randomized into three groups of 15 participants each. Each group received either normal saline eye drops or lubricant eye drops. For each group, one drop was applied three times a day for six weeks. All participants applied the normal saline solution for the first week. The outcomes assessed were the Arab-ocular surface disease index (OSDI) scores and clinical tests including tear break-up time test (TBUT), corneal fluorescein staining (CFS), and lissamine green conjunctival staining (LGS) at weeks 1, 3, and 6.
Results: Both formulations exhibited a significant improvement in Arab-OSDI scores from visit 2 at follow-up time points (p < 0.001). TBUT, CFS, and LGS showed an improvement in both the 0.15% SH and 0.38% SH groups (p < 0.05). SH 0.38% had a greater improvement in the proportion of evaporative dry eye from visit 2 to visit 5 (p = 0.001).
Conclusion: Lubricant eye drops are beneficial for alleviating the symptoms of dry eye. There was no noticeable difference in the effectiveness of these formulations in relieving symptoms and changing any of the objective signs that were assessed. Improved EDE outcomes occurred with SH 0.38% eye drops, observed between visit 2 and visit 5.
Identification of circRNA-miRNA-mRNA regulatory network associated to the autism spectrum disorder in children through integrated bioinformatics analysis
Authors: Pour Najmeh Nezamabadi , Ahmadi Kambiz , Ebrahimi Seyed Omar , Reiisi Somayeh , Jahanara Abbas
Published: September 2023

Background: Autism spectrum disorder (ASD) is a complex neurological disability with multifactorial etiology. ASD is described by behavior, speech, language, and communication defects. CircRNA is a type of ceRNA that plays an important role in modulating microRNAs (miRNA) in several disorders. However, the potential role of the circRNA/miRNA/mRNA regulatory network in the pathogenesis of ASD is not fully understood. Therefore, this study aimed to create a circRNA/miRNA/mRNA network associated with ASD to cast light on the pathogenesis of ASD.
Methods: CircRNA expression profile data were recruited from Gene Expression Omnibus datasets, and the differentially expressed circRNAs (DEcircRNAs) were identified. Then, miRNAs modulated by these circRNAs were predicted and overlapped with differentially expressed miRNAs. Next, the potentially involved genes were identified by overlapping predicted targets, and differentially expressed genes. The enrichment analysis was performed, and a PPI network was projected. Subsequently, ten key genes were selected from the network. Furthermore, a circRNA/miRNA/mRNA regulatory network was constructed, and probable molecules and drugs with potential anti-ASD effects were predicted.
Results: 11 DEcircRNAs and 8 miRNAs regulated by 4 circRNAs were identified as being significantly involved. Subsequently, gene enrichment analysis of 71 overlapped mRNA regulated by these miRNAs showed that they are mostly associated with hippocampal synaptogenesis, neurogenesis, and axon guidance. Additionally, two high-score compounds, GSK3β inhibitor (SB216763) and dexamethasone, and three drugs (haloperidol, nystatin, paroxetine) were confirmed as potential therapeutic options for ASD.
Conclusion: The results of this study may help gain deeper insight into the pathogenesis of the circRNA/miRNA/mRNA regulatory network in ASD, providing potential therapeutic management options.
A protease from Moringa oleifera Lam exhibits plasmin like activity of blood clot solubilization under ex-vivo and in-vitro hydrolysis of fibrin
Authors: Sawetaji Sawetaji , Aggarwal Kamal Krishan
Published: September 2023

Thrombosis is the formation of abnormal blood clots in the blood vessels that obstruct blood flow and lead to cause thrombosis. Current treatments for thrombosis are associated with serious side effects. Therefore there is a need for alternative natural therapy. To isolate and characterize fibrinolytic protease from M.oleifera and evaluation of its fibrinolytic efficiency. Fresh leaves of Moringa oleifera Lam were taken, fibrinolytic protease was isolated and characterized for its potential to solubilize fibrin under in-vitro conditions and its blood clot solubilization efficiency under ex-vivo experiments. The isolated protease showed a single protein band on native-PAGE. It showed optimum fibrinolytic activity at pH 8.0, 37 oC at 50µg concentration. Its fibrinolytic activity was also confirmed by fibrin zymography. Km and Vmax of isolated protease was determined by the Lineweaver Burk plot. The isolated protease could solubilize 96.41% of blood clot by 96 hrs under ex-vivo conditions. In-vitro fibrin hydrolysis and ex-vivo blood clot solubilization activities shown by isolated protease from leaves of Moringa oleifera Lam suggest its fibrinolytic potential to dissolve blood clots. Being a natural molecule and from a dietary plant it can be explored as an alternative natural therapy against thrombosis.
Large-scale genome-centric metagenomics reveals the prevalence of lysogenic virus-host interactions in activated sludge
Authors: Zhang Xu-Xiang , Li Jie , Wang Depeng , He Xiwei , Liu Peng , Ren Hongqiang
Published: September 2023

Bacteriophages are the most common organisms in the activated sludge (AS) of wastewater treatment plants (WWTPs), playing an important role in maintaining or regulating the microbial community. However, lysogenic virus-host interactions in artificial AS systems remain poorly understood. To comprehensively characterize integrated prophages in AS, we reconstructed metagenome-assembled genomes (MAGs) from 44 full-scale WWTPs across five countries. Results showed that lysogenic virus-host interactions were prevalent in AS systems, with > 55% lysogenic MAGs (lysogens), and Proteobacteria (13.8%) and Bacteroidota (12.7%) were the most common lysogenic bacterial phyla. Gene-sharing network analysis revealed that most prophages (~ 99%) could not be categorized into the recognized viral genera, indicating that AS is an untapped source of novel and diverse viruses. The community structure and diversity of lysogens and parasitic prophages in AS systems showed evidently spatial variations, but AS systems from different locations possessed common functional lysogenic microorganisms. In addition, the parasitic prophages detected within lysogens were active and inducible. The active prophages were found to be able to lyse the microorganisms involved in the removal of organic matter, nitrogen, and phosphorous. To our best knowledge, this is the first study to reveal the predominance of lysogenic virus-host interactions in AS systems by large-scale genome-centric metagenomics, which enhanced our understanding of the pivotal role of the prophages in the AS microbiome.
SIRT1 inhibits Smad3 acetylation by reducing Lnc CRNDE transcription and inhibits BV2 cell overactivation to promote SCI neurological repair
Authors: Chen Hongbo , Li Qiaofen , Yu Huimin , Pan Zhipeng , Zhang Bingxue , Yan Na , Zhang Yuying , Li Guiliang , Lv Shaokun , Lv Meifen
Published: September 2023

Methods
We established a BV2 cell activation model by in vitro lipopolysaccharide (LPS) treatment and a rat SCI model according to physical injury. We used HE staining, Luxol solid blue staining and Nissl staining to observe the spinal cord structure, RT‒qPCR to detect SIRT1 mRNA and Lnc CRNDE expression, western blotting to detect SIRT1 protein, BV2 cell activation protein marker (Iba-1) and autophagy-related protein (LC3; Beclin-1; P62) expression, immunoprecipitation reaction to detect the relationship between Smad3 and SIRT1 binding, RNA binding protein immunoprecipitation (RIP) to detect the relationship between Smad3 and CRNDE, dual luciferase reporter gene to verify the transcriptional regulation of CRNDE by Smad3, and immunofluorescence staining to detect the coexpression of BV2 cell activation marker (Iba-1) and autophagy marker (P62).
Results
SIRT1 was expressed at low levels in SCI and LPS-treated BV2 cells from SCI rats. Overexpression of SIRT1 promoted BV2 cell autophagy, inhibited BV2 cell overactivation, alleviated the pathological conditions of spinal cord congestion, edema and structural damage after SCI, improved BBB scores, increased neuronal numbers and promoted myelin regeneration. SIRT1 could inhibit Lnc CRNDE transcription by reducing Smad3. SIRT1 inhibits Lnc CRNDE transcription by reducing Smad3 acetylation and inhibiting its nuclear localization. Overexpression of CRNDE reversed the protective effect on SCI exhibited by SIRT1, and knockdown of CRNDE inhibited BV2 cell overactivation and promoted SCI repair.
Conclusion
SIRT1 promotes SCI repair by reducing Smad3 acetylation and inhibiting its nuclear localization to suppress Lnc CRNDE transcription and inhibit BV2 cell overactivation.
Ubiquitin-conjugating enzyme 2C (UBE2C) plays as a cancer diagnostic and prognostic marker: A Bioinformatics pan-cancer analysis
Authors: Jalali Pooya , Samei Amir , Rezaie Malihe , Shahmoradi Arvin , Pashizeh Fatemeh , Salehi Zahra
Published: September 2023

Background
The diverse and complex attributes of cancer have made it a daunting challenge to overcome globally and remains to endanger human life. Detection of critical cancer-related gene alterations in solid tumor samples better defines patient diagnosis and prognosis, and indicates what targeted therapies must be administered to improve cancer patients’ outcome.
Materials and Methods
To identify genes which have aberrant expression across different cancer types, differential expressed genes were detected within the TCGA datasets. Subsequently, the DEGs common to all pan cancers were determined. Furthermore, various methods were employed to gain genetic alterations, co-expression genes network and protein-protein interaction (PPI) network, pathway enrichment analysis of common genes. Finally, the gene regulatory network was constructed.
Results
Intersectional analysis identified UBE2C as a common DEG between all 28 types of studied cancers. Upregulated UBE2C expression was significantly correlated with OS and DFS of 10 and 9 types of cancer patients. Also, UBE2C can be a diagnostic factor in CESC, CHOL, GBM, and UCS with AUC = 100% and diagnose 19 cancer types with AUC ≥ 90%. A ceRNA network constructed including UBE2C, 41 TFs, 10 shared miRNAs, and 21 circRNAs and 128 lncRNAs.
Conclusion
In summary, UBE2C can be a theranostic gene which may serve as a reliable biomarker in diagnosing cancers, improving treatment responses and increasing the overall survival of cancer patients and can be a promising gene to be target by cancer drugs in the future.
Complications of Delayed Diagnosis and Challenges: Hereditary Spherocytosis with Hepatocellular Jaundice, a Case Report from Ethiopia
Authors: Mekonnen Sintayehu , Adefris Dereje , Bati Abdi , Azmeraw Daniel , Teshome Eliud , Farris Hawi
Published: September 2023

Hereditary spherocytosis is a rare genetic disorder of the RBC membrane that is characterized by anemia, jaundice, and splenomegaly; however, in the absence of family history and with unusual clinical presentation, the diagnosis might not be made until later in life. Here, we present a challenging case of genetically proven hereditary spherocytosis that involves the SPTB gene in a 23-year-old female patient who had repeated medical visits for episodic jaundice, where the delay in diagnosis led to several complications.
The impact of accounting information quality and financing constraints on innovation input of Chinese private enterprises
Authors: Tang Liqing
Published: September 2023

With the advancement of economic globalization and the gradual intensification of competition in international market, enterprises need to constantly enhance their comprehensive strength to consolidate market position and improve the internal innovation ability, which is also a new mission given to enterprises under the new era. At present, the innovation activities of Chinese enterprises are faced with many difficulties, such as long investment cycle time, too much investment capital, high risk, short-term income is not obvious, and so on. However, if enterprises use agents to promote enterprise innovation, the financing constraints caused by the current market agency costs and internal and external information asymmetry greatly hinder the process of independent innovation of private enterprises. Therefore, it is of great significance to study the influencing factors of private enterprises' input in indigenous innovation to improve their independent innovation ability and promote their further development.
Blockchain and human resource management: Emerging themes and research agenda
Authors: Zirar Araz
Published: September 2023

The potential of Blockchain to solve human resource management (HRM) pain points is increasingly being pitched. This review explores the value of Blockchain in HRM. A systematic search of 622 documents from Scopus, Web of Science, Business Source Complete, ABI/INFORM Collection, and Emerald Insight yielded 25 articles on Blockchain's potential for HRM. The analysis suggests that (1) rather than comparing HR professionals' awareness of blockchain technology against non-HR professionals, it is necessary to inquire about HR professionals' awareness level of the technology; (2) while Blockchain has potential for HRM, the advantages of Blockchain in HRM are often perceived rather than actual, and a convincing rationale for adoption is lacking; (3) the cited contributions of Blockchain to HRM assume a range of assumptions about Blockchain in the cryptocurrency world and reflect a high-level understanding of what Blockchain is or may be; (4) HR professionals and their organisations must examine the likely consequences of blockchain adoption, such as the need for highly sought-after skills, skill distribution between Blockchain and workers, and workers with obsolete skills, and (5) research focus should be on how to deal with the challenges of blockchain adoption rather than the perks that an organisation and HR professionals may gain. Research questions are offered after each theme to encourage further research.
WITHDRAWN: General Solutions with Hidden Variables to the Riccati Equation and Second-Order Linear ODEs
Authors: Zhao Ji-Xiang
Published: September 2023

By using elementary quadrature method, analytical general solutions with hidden variables to the Riccati equation and second-Order Linear ODEs are presented in this paper, which provide a possible mathematical criterion for the hidden variable model in various fields.
MEDIATIZATION: ENCOURAGING THE EXISTENCE OF SUBCULTURES THROUGH THE MEDIA
Authors: Yudha Reza Praditya
Published: September 2023

This article discusses Citayam Fashion Weeks from a mediatization perspective. The purpose of this article is to examine the socio-cultural dynamics behind the Citayam Fashion Weeks phenomenon. This article argues that the ease of using the media has encouraged the birth of subcultures into the public sphere. The findings of the study show that the media make it easier for subcultures to gain existence. The logic of the media then creates a shift in meaning and banalization of the identity of Citayam Fashion Weeks as a subculture. The existence of subcultures is then related to their ability to adapt to large figurations in the surrounding environment.
KELOMPOK ISLAM NURCU DAN NEGARA: PENERIMAAN MUSLIM ATAS SEKULARISME
Authors: Khamami Akhmad Rizqon
Published: September 2023

This article explores the Nurcu's response to the implementation of secularization in the Republic of Turkey in the 20th century. The implementation of secularism in Turkey brought about three groups: Kemalists, Islamists, and Nurcu. While the Kemalists became the main proponents of secularism, and the Islamists strongly rejected it, a third group emerged, that is the Nurcu. The Nurcu is an association of followers of the teachings of Said Nursi, a Turkish scholar and thinker. This article assumes that the Nurcu accepts secularism, and actually supports it. They believe that secularization is not in contradiction to Islamic teachings. The acceptance of secularism subsequently leads the Nurcu's responses to state, democracy, role of the people, and the economic system adopted by the state. This study found that the Nurcu group enjoyed social and political openness, democracy and economic liberalization in Turkey, despite tensions between the Nurcu and the Kemalist.
The Prevalence of PTSD Among an Online Sample of US Familial Dementia Caregivers
Authors: Mashinchi Genna M , Hicks Emily C. , Heppner Hannes
Published: September 2023

The increased prevalence of dementia has increased the number of caregivers. Familial caregivers can suffer emotionally as a result of caregiving. Despite caregiver organizations campaigning for awareness of caregiver trauma, more research examining PTSD among US familial dementia caregivers is needed. The present study surveyed an online sample of US familial dementia caregivers to gather a prevalence rate of PTSD. We hypothesized that these caregivers experience an equal or greater prevalence of PTSD compared to the general US population, nurses, emergency medical responders, and veterans. An online survey containing the PTSD-Civilian Checklist (PCL-C) was administered to familial dementia caregivers across the US. Data (n = 23) revealed that 26.1% of participants surveyed screened positive for PTSD criteria. Consistent with our hypothesis, this percentage is greater than the prevalence rates for the general US population and is similar to the prevalence rates for emergency first responders, nurses, and even US military veterans. These findings can greatly benefit US dementia caregivers by spreading awareness and helping allocate resources to assist those struggling with PTSD.
Impact of economic policy uncertainty on audit fees: Evidence from Chinese listed companies
Authors: Cheng Ming , Sutunyarak Chonlavit
Published: September 2023

High economic policy uncertainty in China amplifies operational risks and managerial pressure for businesses while driving the demand for independent audits. However, previous studies have paid limited attention to the impact of economic policy uncertainty on audit fees. This study aims to examine the impact of economic policy uncertainty on audit fees and to test whether the internal control quality will moderate this association at the firm level. This study employs the economic policy uncertainty index to measure uncertainty. The paper examines 3,469 Chinese A-share listed companies from 2007 to 2020, using STATA 17 for fixed-effects regression on panel data. The results show a robust positive association between economic policy uncertainty and audit fees (β = 0.0302, p < 0.001). However, this association is weaker for companies with better internal control quality (β = –0.0229, p < 0.001), suggesting that effective internal controls can mitigate the impact of economic policy uncertainty on audit fees. Furthermore, the findings indicate that this positive association is weaker for state-owned companies compared to non-state-owned companies (β = –0.0170, p < 0.001). At the same time, the study does not establish a significant difference in the positive association between TOP 10 and non-TOP 10 accounting firms. This study examines the adverse effects of economic policy uncertainty from the perspective of audit fees. The results have implications for stakeholders, including the need for companies to establish effective internal controls and good government-business relationships and for governments to reduce economic policy uncertainty and increase transparency in their policies.
Multibody system dynamics modelling and simulation of a high-speed train for its suspension optimization
Authors: Wu Yongming , Liu Shiping , Wu Dazhuo , Wang Wenlin
Published: September 2023

Multibody system (MBS) dynamics modelling and simulation of high-speed train system is essential and meaningful in the context of train dynamics research and product design. In this work, MBS modelling of a CRH high-speed train is carried out, in the modelling process, nonlinear geometry of wheel-rail contact, nonlinear characteristics of the suspensions and nonlinear railroad irregularities are fully considered. With the obtained MBS model, simulation is performed and demonstrates that critical speed of the CRH train is 378 km/h, vertical ride comfort of the CRH train is in “Excellent” level and lateral ride comfort is in “Good” level, in addition, RMS derailment coefficient of the train is less than 0.5 which means curving performance of the train is good. Agreement of simulation result with actual test or running data validates the established MBS model, thus, the MBS model developed in this study is accurate and would be useful in the next step vibration analysis and suspension optimization of the CRH train.
Magnetic smart materials enabled micro-pump: design, modeling, and performance analysis
Authors: Jiang Huihuang , Hu Hao , Ullakko Kari , Liu Xiaojie , He Shan , Guo Donghui
Published: September 2023

Smart materials have gained significant attention in various industries due to their exceptional properties and unique capabilities. Among these materials, smart magnetic materials have emerged as a promising candidate with the ability to sense and respond to environmental changes. This paper presents a novel micro-pump structure, fabricated using 0.18 µm integrated technology, which can be integrated into microfluidic systems and MEMS devices. In this study, the design and manufacturing process of the micro-pump, utilizing magnetic smart materials, are presented. The characteristics of the smart material and the micro-pump's performance are analyzed through simulations. The proposed micro-pump design incorporates one-way valves and has demonstrated improved efficiency and enhanced performance compared to conventional designs. The results of this study provide a foundation for further research and development of magnetic smart material-based micro-pumps and their potential applications in various fields, including lab-on-a-chip devices and biomedical engineering.
Experimental study on rolling segregation of cement stabilized macadam
Authors: Wang Zhibin , Wang Xiaosen , Qiu Wenli , Meng Huilin , Li Haidong
Published: September 2023

Indoor simulated mixing building storage bin unloading, forming conical piles. The effects of falling distance, mixing time and water content on rolling segregation of cement-stabilized macadam were analyzed by three indexes, including repose Angle of pile, key sieve passing rate and cement dosage. The results show that the segregation of mixture can be increased significantly with the increase of drop distance. The separation degree of the mixture can be effectively controlled by controlling the unloading distance within 70 cm. Mixing time has little effect on the Angle of rest and gradation segregation of the mixture, but has significant effect on the distribution uniformity of cement. Indoor mixing time should reach more than 45 s. When the mixing water content is less than the optimal water content, the segregation of the mixture is serious. When the moisture content increased to 1.5 %, the mixture showed obvious bleeding phenomenon. The mixture shall not be lower than the optimal moisture content before the crushing is completed, and the moisture content increase shall not exceed 1.5 % of the optimal moisture content as far as possible.
Study on the fatigue performance of steel-composite grillage deck of large-span arch bridge
Authors: Huang Longxian , Liao Nengwu , Li Hong , Zhang Liangliang
Published: September 2023

In this paper, based on the finite element numerical method, an arch bridge with a main span of 252m was selected as the engineering background, and three standard grillage segments of the bridge deck in the derrick area were selected to establish a refined finite element model, and the fatigue characteristics of the steel-composite grillage deck were studied. The fatigue calculation mode III in General Specification for Design of Highway Bridges and Culverts (JTG D60-2015) was loaded into the finite element model for calculation, and the fatigue key points were determined, the stress time course curves were extracted, and the fatigue damage calculation was carried out. The results indicate that the most unfavorable stress point of the steel-composite structure model is at the bottom plate (outside) of the intersection of the middle longitudinal beam and the secondary crossbeam under the longitudinal loading of vehicle load in the transverse position; the stress amplitude of the maximum critical point in the longitudinal bridge stress course under the standard fatigue vehicle model is less than the fatigue strength limit of the normal amplitude, which meets the design requirements.
Microstructure and properties of iron-based surfacing layer based on JmatPro software simulation calculation
Authors: Chen Zhongbing , Yu Yunhe , Hou Jixin , Zhu Ping , Zhang Jianlin
Published: September 2023

The material performance simulation software JMatPro was used to simulate and calculate equilibrium phase structure and properties of a mixture formed by mixing different proportions of Norem02 alloy and F304 stainless steel. The results show that in the range of 500-1300 °C, equilibrium phases of the mixture include Austenite and Ferrite, and the carbides include M2(C,N), M6C, M7C3 and M23C6. As the content of F304 in the mixture increases, the phase transition temperature A1 of the mixture decreases, and A3 first decreases and then increases; in the equilibrium phase, the content of Austenite and Ferrite increases, and the content of carbide decreases until the carbides M7C3 and M6C gradually disappear. Density, coefficient of linear expansion, thermal conductivity, Young’s modulus, Poisson’s ratio and specific heat capacity of different mixture materials vary with temperature in a complex manner. Strength and hardness of the mixture formed when the content of F304 is 20 % is the highest. The finer the grain size of the mixture, the higher the strength and hardness of the material. Comparing standard physical property data of F304 stainless steel at room temperature with the simulation calculation results, it is found that the error is small, which verifies the accuracy of the simulation calculation results.
Influence of structural irregularity on the q-behaviour factor of light-frame timber buildings by means of incremental dynamic analysis
Authors: Mazelli Alessandro , Bedon Chiara , Morassi Antonino
Published: September 2023

This paper investigates the role of sheathing-to-framing connection ductility in the evaluation of the structural q-behaviour factor for Light-Frame Timber (LFT) buildings, by means of Incremental Dynamic Analyses (IDA). This approach allows to consider nonlinear cyclic behaviour of the walls, which cannot be taken into account with the static approaches used in most of the available literature on LFT buildings. To this aim, Finite Element wall models, preliminary calibrated towards a cyclic full-scale experimental test, are built to study six case-study buildings, both regular and non-regular, with 2, 3 or 4 storeys, which were designed according to Eurocode and Capacity Design provisions. Parametric analyses are performed by varying the displacement-ductility of the panel. Finally, numerical results are discussed in terms of q-behaviour factor, and its sensitivity to structural irregularities, with respect to existing code provisions for timber buildings.
Non-regularity damage evaluation in reinforced concrete structures via fragility curves
Authors: Smiroldo Giovanni , Fasan Marco , Bedon Chiara
Published: September 2023

The present study explores the seismic performance and the expected structural damage in reinforced concrete (RC) frames with progressively increasing in-plan and in-height non-regularity. To this aim, four three-dimensional (3D) buildings characterised by different regularity levels are taken into account. The structures are subjected to seismic input consisting of unscaled real ground motion records, and non-linear dynamic analyses are performed in the Cloud Analysis framework, represented by three different intensity measures (IMs). To evaluate the effect of structural non-regularity, fragility curves are derived based on linear regression models.
Research on the microstructure and properties of iron based alloy coatings based on gradient transition
Authors: Chen Zhongbing , Sun Zhiqiang , Yang Jia , Zhang Jianlin , Yao Xianghong
Published: September 2023

An iron alloy coating with gradient transition was sprayed on F304 stainless steel substrate by plasma spraying method and with a gradient material obtained by the material performance simulation software, and the effect of gradient transition on the microstructure, hardness and wear resistance of the coating was analyzed by comparing with the non gradient transition coating. The results show that both the gradient coating and the non gradient coating have wavy layered structure and are formed by highly flattened deformed particles overlapping each other. Micro metallurgical bonding was observed between the coating particles, and oxides were observed between the coating and the substrate, between the flat particles and inside the flat layer. The coating microstructure includes austenite phase, ferrite phase and carbides. The surface hardness of the gradient coating and the non gradient transition coating are basically similar, but there are obvious steep drop steps between the section hardness of the non gradient coating and the substrate hardness, while the section hardness of the gradient coating and the substrate hardness change gently, and the section hardness of the gradient coating is slightly lower than that of the non gradient coating. The wear rate of gradient coating is similar to that of non gradient coating at 400 °C, but it is lower than that of non gradient transition coating at 350 °C. The reason for reducing hardness changes and improving wear resistance of the coating may be related to the improvement of the microstructure and plasticity of the coating by the gradient layer, but more research is needed.
Role of secondary components in the numerical analysis and in-plane seismic performance assessment of glass curtain walls
Authors: Cella Nicola , Bedon Chiara
Published: September 2023

Glass façades are complex mechanical systems, in which brittle and vulnerable glass panels interact with metal members and secondary components. Under extreme design actions, such as seismic events, glass failure in tension (cracking) or compression (crushing) is a critical condition for structural performance assessment. Compared to full-scale experiments, in this regard, Finite Element (FE) numerical tools can offer a robust support in design. Besides, many primary and secondary façade components should be properly taken into account, because responsible of possible major approximations in their expected mechanical interactions. In this paper, the in-plane seismic response of glass curtain walls is investigated with geometrically accurate and detailed (“MREF”) or simplified and efficient (“MSIMP”) numerical models. Comparative results are critically discussed, based on dynamic numerical simulations, with a primary attention which is focused on the mechanical performance of glass panels.
Effect of heat treatment on microstructure and properties of 10B21 cold-heading steel for fasteners
Authors: Chen Xiaoli , Hou Yu
Published: September 2023

Fasteners are widely used in automobile, electronic and other fields, and the demand for high performance fastener materials is increasingly urgent. 10B21 mild steel has the advantages of good plasticity, obvious heat treatment effect and so on. It is used to produce fasteners below grade 9.8. In this paper, the domestic 10B21 boron steel as the research object, through quenching, tempering and other heat treatment, change its structure from ferrite to tempered martensite, in order to improve the strength and hardness.