bluuebunny/crossref_metadata_2025 huggingface dataset:
Selection of Rattus norvegicus cumulus–oocyte complex for vitrification by brilliant cresyl blue
Authors: Oliveira Iaskara , Fisch Joana , Gomes Juliana , Lopes Rui Fernando Felix , Oliveira Alexandre Tavares Duarte de
Published: July 2023

The influence of the method of evaluating developmentally competent oocytes on their viability after cryopreservation still needs to be better understood. The objective of this study was to determine the cleavage and embryo developmental rates after parthenogenetic activation of cumulus–oocyte complexes (COCs) selected by different concentrations of brilliant cresyl blue (BCB) and cryopreservation. In the first experiment, COCs were separated into groups and incubated for 1 h in medium containing BCB (13 μM, 16 μM, or 20 μM). The control group was not exposed to BCB staining. In the second experiment, COCs were divided into four groups: 13 μM BCB(+), 13 μM BCB(−), fresh control (selected by morphologic observation and immediately in vitro matured) and vitrified control (selected by morphologic evaluation, vitrified, and in vitro matured). In the first experiment, the 13 μM BCB group displayed greater development rates at the morula stage (65.45%, 36/55) when compared with the other groups. In the second experiment, cleavage (47.05%, 72/153) and morula development (33.55%, 51/153) of the control group of fresh COCs were increased compared with the other groups. However, when comparing morula rates between vitrified COC control and BCB(+) groups, the BCB(+) group had better results (19.23%, 5/26 and 64.7%, 11/17, respectively). Our best result in rat COC selection by BCB staining was obtained using a concentration of 13 μM. This selection could be a valuable tool to improve vitrification outcomes, as observed by the BCB(+) group that demonstrated better results compared with the vitrified COC control.
Developing feasible healthy diets for Ethiopian women of reproductive age: a linear goal programming approach
Authors: Bekele Tesfaye Hailu , van Rooijen Maike , Gerdessen Johanna C , Brouwer Inge D , Feskens Edith JM , Trijsburg Laura , Alemayehu Dawit , de Vries Jeanne HM
Published: July 2023

Objective:

To develop a healthy diet for Ethiopian women closely resembling their current diet and taking fasting periods into account while tracking the cost difference.

Design:

Linear goal programming models were built for three scenarios (non-fasting, continuous fasting and intermittent fasting). Each model minimised a function of deviations from nutrient reference values for eleven nutrients (protein, Ca, Fe, Zn, folate, and the vitamins A, B1, B2, B3, B6, and B12). The energy intake in optimised diets could only deviate 5 % from the current diet.

Settings:

Five regions are included in the urban and rural areas of Ethiopia.

Participants:

Two non-consecutive 24-h dietary recalls (24HDR) were collected from 494 Ethiopian women of reproductive age from November to December 2019.

Results:

Women’s mean energy intake was well above 2000 kcal across all socio-demographic subgroups. Compared to the current diet, the estimated intake of several food groups was considerably higher in the optimised modelled diets, that is, milk and dairy foods (396 v. 30 g/d), nuts and seeds (20 v. 1 g/d) and fruits (200 v. 7 g/d). Except for Ca and vitamin B12 intake in the continuous fasting diet, the proposed diets provide an adequate intake of the targeted micronutrients. The proposed diets had a maximum cost of 120 Ethiopian birrs ($3·5) per d, twice the current diet’s cost.

Conclusion:

The modelled diets may be feasible for women of reproductive age as they are close to their current diets and fulfil their energy and nutrient demands. However, the costs may be a barrier to implementation.
Assessment of health-care workers’ attitudes, knowledge, and skills in the care of critically ill Muslim children in New York
Authors: Ijaz Nadir , Hassan Amir , Ghannoum Sarah , Choudhury Tarif , Piracha Natasha
Published: July 2023

Objectives

Pediatric health-care workers often care for families of minority religious backgrounds, but little is known about their perspective in providing culturally and spiritually appropriate care for Muslim patients. We aimed to (1) characterize the attitudes, knowledge, and skills of health-care workers in the care of critically ill Muslim children and (2) evaluate preferences for different educational interventions to improve care of critically ill Muslim children.

Methods

We administered a single-center, cross-sectional, 33-question, electronic survey of interdisciplinary health-care workers in a large pediatric intensive care unit in New York City to characterize their attitudes, knowledge, and skills in caring for critically ill Muslim children.

Results

Of 413 health-care workers surveyed, there were 109 (26%) respondents. Participants responded correctly to 51.7 ± 22.2% (mean ± SD) and 69.2 ± 20.6% of background knowledge and clinical skills questions, respectively. Only 29.8% of participants perceived adequate institutional resources to provide culturally competent care to Muslim patients and their families. Participants identified end-of-life care (47.5%) and bioethical concerns (45%) as needed areas for additional institutional resources. When asked about support to aid in caring for Muslim patients, 43.4% of participants requested a team of Muslim health-care workers to provide guidance. Participants most often requested video-based training modules (32.5%) and written materials (30%) as potential educational interventions.

Significance of results

We identify gaps in health-care worker knowledge and skills in the care of the critically ill Muslim child. We also describe possible areas for intervention to facilitate culturally and spiritually appropriate care delivery to Muslim children and families.
Anglican Confirmation: an Unfinished Reform
Authors: Buchanan Colin
Published: July 2023

Confirmation, one of the seven sacraments in Lombard’s schedule, was retained by the English Reformers, but not as a sacrament and without any distinctive quasi-sacramental grace attributed to it. It became a ceremony to complete the catechizing process for children who, having been baptized as infants, were at the age of discretion now to come to holy communion. The reformers thought that a post-baptismal laying on of hands had been practised from apostolic times, and so commended the ceremony for their church practice. This requirement enabled later generations, such as Gregory Dix, to bid up its significance, teaching that confirmation completes baptism, and thus that water-baptism is of itself incomplete. The underlying premise has been that from the apostles onwards a requisite second initiatory ceremony invariably followed baptism. Both Bible and early church history contradict this thesis, not least the eight post-apostolic authors of the first two centuries who mention baptism. All eight testify to the use of water without any further ceremony. Thus, any insistence upon a two-stage sacramental initiation today lacks historical foundations; Anglicans ought to review residual texts and practices which reflect such a pattern.
Early neurodevelopment in the offspring of women enrolled in a randomized controlled trial assessing the effectiveness of a nutrition + exercise intervention on the cognitive development of 12-month-olds
Authors: Mortaji Neda , Krzeczkowski John , Atkinson Stephanie , Amani Bahar , Schmidt Louis A. , Van Lieshout Ryan J.
Published: July 2023

Experimental data on the effects of lifestyle interventions on fetal neurodevelopment in humans remain scarce. This study assessed the impact of a pregnancy nutrition+exercise intervention on offspring neurodevelopment at 12 months of age. The Be Healthy in Pregnancy (BHIP) randomized controlled trial (RCT) randomly assigned pregnant persons with stratification by site and body mass index (BMI) to bi-weekly nutrition counselling and high dairy protein diet, walking goal of 10,000 steps/day plus usual prenatal care (UPC; intervention group) or UPC alone (control group). This study examined a subset of these mothers (> 18 years, singleton pregnancy, BMI <40 kg/m2, and enrolled by ≤12 weeks gestation) and their infants (intervention = 42, control = 32), assessing cognition, language, motor, social-emotional, and adaptive functioning at 12 months using the Bayley Scales of Infant and Toddler Development third edition (BSID-III) as the outcome measure. We also examined if maternal factors (prepregnancy BMI, gestational weight gain (GWG)) moderated associations. Expressive language (MD = 9.62, 95% CI = (9.05–10.18), p = 0.03, ƞ2p = 0.07) and general adaptive composite (GAC) scores (MD = 103.97, 95% CI = (100.31–107.63), p = 0.04, ƞ2p = 0.06) were higher in infants of mothers in the intervention group. Effect sizes were medium. However, mean cognitive, receptive language, motor, and social-emotional scale scores did not differ between groups. A structured and monitored nutrition+exercise intervention during pregnancy led to improved expressive language and general adaptive behavior in 12-month-olds, but not cognitive, receptive language, motor, or socioemotional functioning. While these experimental data are promising, further research is needed to determine the clinical utility of nutrition+exercise interventions for optimizing infant neurodevelopment.
Countering Misinformation
Authors: Roozenbeek Jon , Culloty Eileen , Suiter Jane
Published: July 2023

Abstract: Developing effective interventions to counter misinformation is an urgent goal, but it also presents conceptual, empirical, and practical difficulties, compounded by the fact that misinformation research is in its infancy. This paper provides researchers and policymakers with an overview of which individual-level interventions are likely to influence the spread of, susceptibility to, or impact of misinformation. We review the evidence for the effectiveness of four categories of interventions: boosting (psychological inoculation, critical thinking, and media and information literacy); nudging (accuracy primes and social norms nudges); debunking (fact-checking); and automated content labeling. In each area, we assess the empirical evidence, key gaps in knowledge, and practical considerations. We conclude with a series of recommendations for policymakers and tech companies to ensure a comprehensive approach to tackling misinformation.
Thank You to Our 2022 Reviewers
Authors: Parrish Julia , Schensul Jean , Buchanan Paula , Gupta Shobhana , Haklay Muki , Roop Heidi , Semmens Kathryn , Shukla Roopam
Published: July 2023

Key Points

The editors thank the 2022 peer reviewers
Substorm Impact on Dayside Ionospheric Currents
Authors: Elhawary R. , Laundal K. M. , Reistad J. P. , Madelaire M. , Ohma A.
Published: July 2023

Ionospheric dayside dynamics is strongly controlled by the interaction between the Interplanetary Magnetic Field (IMF) and the Earth's magnetic field near the dayside magnetopause, while nightside ionospheric dynamics depends mainly on magnetotail activity. However, we know little about the influence of magnetotail activity on the dayside ionospheric dynamics. We investigate this by performing superposed epoch analyses of ground magnetic field data for substorms occurring during northward IMF. In such substorms, dayside reconnection is minimized, allowing us to separate the effects of the magnetotail activity on the dayside current system. We find that as nightside activity elevates, the dayside ionospheric current elevates. Our analyses indicate that the lobe cells are less distinct before onset than during non‐substorm northward IMF conditions. They become more pronounced after onset, possibly due to magnetospheric reconfiguration or a remote effect of the nightside current. We discuss possible mechanisms that may explain our observations.
The East‐West Asymmetry of Particle Intensity in Energetic Storm Particle Events
Authors: Ding Zheyi , Li Gang , Santa Fe Dueñas Adolfo , Ebert Robert W. , Wijsen Nicolas , Poedts Stefaan
Published: July 2023

We examine the East‐West asymmetry of the peak intensity in energetic storm particle (ESP) events using the improved Particle Acceleration and Transport in the Heliosphere model. We find that injection efficiency peaks east of the nose of coronal mass ejection shock where the shock exhibits a quasi‐parallel geometry. We show that the peak intensity at the eastern flank is generally larger than that at the western flank and it positively correlates with the injection efficiency. We also examine this asymmetry for heavy ions, which depends sensitively on the ion energy. Comparison between the modeling results with the measurements of ESP events at 1 au shows a reasonable agreement. We suggest that the injection efficiency can be a primary factor leading to the East‐West asymmetry of the peak intensity in ESP events. Additionally, the charge‐to‐mass (Q/A) dependence of the maximum particle energy affects this asymmetry for heavy ions.
Culture expansion of CAR T cells results in aberrant DNA methylation that is associated with adverse clinical outcome
Authors: Salz Lucia , Seitz Alexander , Schäfer Daniel , Franzen Julia , Holzer Tatjana , Garcia-Prieto Carlos A. , Bürger Iris , Hardt Olaf , Esteller Manel , Wagner Wolfgang
Published: July 2023

Chimeric antigen receptor (CAR) T cells provide new perspectives for treatment of hematological malignancies. Manufacturing of these cellular products includes culture expansion procedures, which may affect cellular integrity and therapeutic outcome. In this study, we investigated culture-associated epigenetic changes in CAR T cells and found continuous gain of DNAm, particularly within genes that are relevant for T cell function. Hypermethylation in many genes, such as TCF7, RUNX1, and TOX, was reflected by transcriptional downregulation. 332 CG dinucleotides (CpGs) showed an almost linear gain in methylation with cell culture time, albeit neighboring CpGs were not coherently regulated on the same DNA strands. An epigenetic signature based on 14 of these culture-associated CpGs predicted cell culture time across various culture conditions. Notably, even in CAR T cell products of similar culture time higher DNAm levels at these CpGs were associated with significantly reduced long-term survival post transfusion. Our data demonstrate that cell culture expansion of CAR T cells evokes DNA hypermethylation at specific sites in the genome and the signature may also reflect loss of potential in CAR T cell products. Hence, reduced cultivation periods are beneficial to avoid dysfunctional methylation programs that seem to be associated with worse therapeutic outcome.
Mcr-dependent methanogenesis in Archaeoglobaceae enriched from a terrestrial hot spring
Authors: Buessecker Steffen , Chadwick Grayson L , Quan Melanie E , Hedlund Brian P , Dodsworth Jeremy A , Dekas Anne E
Published: July 2023

The preeminent source of biological methane on Earth is methyl coenzyme M reductase (Mcr)-dependent archaeal methanogenesis. A growing body of evidence suggests a diversity of archaea possess Mcr, although experimental validation of hypothesized methane metabolisms has been missing. Here, we provide evidence of a functional Mcr-based methanogenesis pathway in a novel member of the family Archaeoglobaceae, designated Methanoglobus nevadensis, which we enriched from a terrestrial hot spring on the polysaccharide xyloglucan. Our incubation assays demonstrate methane production that is highly sensitive to the Mcr inhibitor bromoethanesulfonate, stimulated by xyloglucan and xyloglucan-derived sugars, concomitant with the consumption of molecular hydrogen, and causing a deuterium fractionation in methane characteristic of hydrogenotrophic and methylotrophic methanogens. Combined with the recovery and analysis of a high-quality M. nevadensis metagenome-assembled genome encoding a divergent Mcr and diverse potential electron and carbon transfer pathways, our observations suggest methanogenesis in M. nevadensis occurs via Mcr and is fueled by the consumption of cross-fed byproducts of xyloglucan fermentation mediated by other community members. Phylogenetic analysis shows close affiliation of the M. nevadensis Mcr with those from Korarchaeota, Nezhaarchaeota, Verstraetearchaeota, and other Archaeoglobales that are divergent from well-characterized Mcr. We propose these archaea likely also use functional Mcr complexes to generate methane on the basis of our experimental validation in M. nevadensis. Thus, divergent Mcr-encoding archaea may be underestimated sources of biological methane in terrestrial and marine hydrothermal environments.
FOXM1 is critical for the fitness recovery of chromosomally unstable cells
Authors: Pan Fan , Chocarro Sara , Ramos Maria , Chen Yuanyuan , Alonso de la Vega Alicia , Somogyi Kalman , Sotillo Rocio
Published: July 2023

Tumor progression and evolution are frequently associated with chromosomal instability (CIN). Tumor cells often express high levels of the mitotic checkpoint protein MAD2, leading to mitotic arrest and cell death. However, some tumor cells are capable of exiting mitosis and consequently increasing CIN. How cells escape the mitotic arrest induced by MAD2 and proliferate with CIN is not well understood. Here, we explored loss-of-function screens and drug sensitivity tests associated with MAD2 levels in aneuploid cells and identified that aneuploid cells with high MAD2 levels are more sensitive to FOXM1 depletion. Inhibition of FOXM1 promotes MAD2-mediated mitotic arrest and exacerbates CIN. Conversely, elevating FOXM1 expression in MAD2-overexpressing human cell lines reverts prolonged mitosis and rescues mitotic errors, cell death and proliferative disadvantages. Mechanistically, we found that FOXM1 facilitates mitotic exit by inhibiting the spindle assembly checkpoint (SAC) and the expression of Cyclin B. Notably, we observed that FOXM1 is upregulated upon aneuploid induction in cells with dysfunctional SAC and error-prone mitosis, and these cells are sensitive to FOXM1 knockdown, indicating a novel vulnerability of aneuploid cells.
Unveiling phase diagram of the lightly doped high-Tc cuprate superconductors with disorder removed
Authors: Kurokawa Kifu , Isono Shunsuke , Kohama Yoshimitsu , Kunisada So , Sakai Shiro , Sekine Ryotaro , Okubo Makoto , Watson Matthew D. , Kim Timur K. , Cacho Cephise , Shin Shik , Tohyama Takami , Tokiwa Kazuyasu , Kondo Takeshi
Published: July 2023

The currently established electronic phase diagram of cuprates is based on a study of single- and double-layered compounds. These CuO2 planes, however, are directly contacted with dopant layers, thus inevitably disordered with an inhomogeneous electronic state. Here, we solve this issue by investigating a 6-layered Ba2Ca5Cu6O12(F,O)2 with inner CuO2 layers, which are clean with the extremely low disorder, by angle-resolved photoemission spectroscopy (ARPES) and quantum oscillation measurements. We find a tiny Fermi pocket with a doping level less than 1% to exhibit well-defined quasiparticle peaks which surprisingly lack the polaronic feature. This provides the first evidence that the slightest amount of carriers is enough to turn a Mott insulating state into a metallic state with long-lived quasiparticles. By tuning hole carriers, we also find an unexpected phase transition from the superconducting to metallic states at 4%. Our results are distinct from the nodal liquid state with polaronic features proposed as an anomaly of the heavily underdoped cuprates.
Breathing porous liquids based on responsive metal-organic framework particles
Authors: Koutsianos Athanasios , Pallach Roman , Frentzel-Beyme Louis , Das Chinmoy , Paulus Michael , Sternemann Christian , Henke Sebastian
Published: July 2023

Responsive metal-organic frameworks (MOFs) that display sigmoidal gas sorption isotherms triggered by discrete gas pressure-induced structural transformations are highly promising materials for energy related applications. However, their lack of transportability via continuous flow hinders their application in systems and designs that rely on liquid agents. We herein present examples of responsive liquid systems which exhibit a breathing behaviour and show step-shaped gas sorption isotherms, akin to the distinct oxygen saturation curve of haemoglobin in blood. Dispersions of flexible MOF nanocrystals in a size-excluded silicone oil form stable porous liquids exhibiting gated uptake for CO2, propane and propylene, as characterized by sigmoidal gas sorption isotherms with distinct transition steps. In situ X-ray diffraction studies show that the sigmoidal gas sorption curve is caused by a narrow pore to large pore phase transformation of the flexible MOF nanocrystals, which respond to gas pressure despite being dispersed in silicone oil. Given the established flexible nature and tunability of a range of MOFs, these results herald the advent of breathing porous liquids whose sorption properties can be tuned rationally for a variety of technological applications.
Structural delineation and computational design of SARS-CoV-2-neutralizing antibodies against Omicron subvariants
Authors: Moriyama Saya , Anraku Yuki , Taminishi Shunta , Adachi Yu , Kuroda Daisuke , Kita Shunsuke , Higuchi Yusuke , Kirita Yuhei , Kotaki Ryutaro , Tonouchi Keisuke , Yumoto Kohei , Suzuki Tateki , Someya Taiyou , Fukuhara Hideo , Kuroda Yudai , Yamamoto Tsukasa , Onodera Taishi , Fukushi Shuetsu , Maeda Ken , Nakamura-Uchiyama Fukumi , Hashiguchi Takao , Hoshino Atsushi , Maenaka Katsumi , Takahashi Yoshimasa
Published: July 2023

SARS-CoV-2 Omicron subvariants have evolved to evade receptor-binding site (RBS) antibodies that exist in diverse individuals as public antibody clones. We rationally selected RBS antibodies resilient to mutations in emerging Omicron subvariants. Y489 was identified as a site of virus vulnerability and a common footprint of broadly neutralizing antibodies against the subvariants. Multiple Y489-binding antibodies were encoded by public clonotypes and additionally recognized F486, potentially accounting for the emergence of Omicron subvariants harboring the F486V mutation. However, a subclass of antibodies broadly neutralized BA.4/BA.5 variants via hydrophobic binding sites of rare clonotypes along with high mutation-resilience under escape mutation screening. A computationally designed antibody based on one of the Y489-binding antibodies, NIV-10/FD03, was able to bind XBB with any 486 mutation and neutralized XBB.1.5. The structural basis for the mutation-resilience of this Y489-binding antibody group may provide important insights into the design of therapeutics resistant to viral escape.
Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration
Authors: Pang Xiaojie , Nawrocki Wojciech J. , Cardol Pierre , Zheng Mengyuan , Jiang Jingjing , Fang Yuan , Yang Wenqiang , Croce Roberta , Tian Lijin
Published: July 2023

While photosynthesis transforms sunlight energy into sugar, aerobic and anaerobic respiration (fermentation) catabolizes sugars to fuel cellular activities. These processes take place within one cell across several compartments, however it remains largely unexplored how they interact with one another. Here we report that the weak acids produced during fermentation down-regulate both photosynthesis and aerobic respiration. This effect is mechanistically explained with an “ion trapping” model, in which the lipid bilayer selectively traps protons that effectively acidify subcellular compartments with smaller buffer capacities – such as the thylakoid lumen. Physiologically, we propose that under certain conditions, e.g., dim light at dawn, tuning down the photosynthetic light reaction could mitigate the pressure on its electron transport chains, while suppression of respiration could accelerate the net oxygen evolution, thus speeding up the recovery from hypoxia. Since we show that this effect is conserved across photosynthetic phyla, these results indicate that fermentation metabolites exert widespread feedback control over photosynthesis and aerobic respiration. This likely allows algae to better cope with changing environmental conditions.
Universal alignment in turbulent pair dispersion
Authors: Shnapp Ron , Brizzolara Stefano , Neamtu-Halic Marius M. , Gambino Alessandro , Holzner Markus
Published: July 2023

Countless processes in nature and industry, from rain droplet nucleation to plankton interaction in the ocean, are intimately related to turbulent fluctuations of local concentrations of advected matter. These fluctuations can be described by considering the change of the separation between particle pairs, known as pair dispersion, which is believed to obey a cubic in time growth according to Richardson’s theory. Our work reveals a universal, scale-invariant alignment between the relative velocity and position vectors of dispersing particles at a mean angle that we show to be a universal constant of turbulence. We connect the value of this mean angle to Richardson’s traditional theory and find agreement with data from a numerical simulation and a laboratory experiment. While the Richardson’s cubic regime has been observed for small initial particle separations only, the constancy of the mean angle manifests throughout the entire inertial range of turbulence. Thus, our work reveals the universal nature of turbulent pair dispersion through a geometrical paradigm whose validity goes beyond the classical theory, and provides a framework for understanding and modeling transport and mixing processes.
Increased body mass index is linked to systemic inflammation through altered chromatin co-accessibility in human preadipocytes
Authors: Garske Kristina M. , Kar Asha , Comenho Caroline , Balliu Brunilda , Pan David Z. , Bhagat Yash V. , Rosenberg Gregory , Koka Amogha , Das Sankha Subhra , Miao Zong , Sinsheimer Janet S. , Kaprio Jaakko , Pietiläinen Kirsi H. , Pajukanta Päivi
Published: July 2023

Obesity-induced adipose tissue dysfunction can cause low-grade inflammation and downstream obesity comorbidities. Although preadipocytes may contribute to this pro-inflammatory environment, the underlying mechanisms are unclear. We used human primary preadipocytes from body mass index (BMI) -discordant monozygotic (MZ) twin pairs to generate epigenetic (ATAC-sequence) and transcriptomic (RNA-sequence) data for testing whether increased BMI alters the subnuclear compartmentalization of open chromatin in the twins’ preadipocytes, causing downstream inflammation. Here we show that the co-accessibility of open chromatin, i.e. compartmentalization of chromatin activity, is altered in the higher vs lower BMI MZ siblings for a large subset ( ~ 88.5 Mb) of the active subnuclear compartments. Using the UK Biobank we show that variants within these regions contribute to systemic inflammation through interactions with BMI on C-reactive protein. In summary, open chromatin co-accessibility in human preadipocytes is disrupted among the higher BMI siblings, suggesting a mechanism how obesity may lead to inflammation via gene-environment interactions.
Tailoring a local acid-like microenvironment for efficient neutral hydrogen evolution
Authors: Zheng Xiaozhong , Shi Xiaoyun , Ning Honghui , Yang Rui , Lu Bing , Luo Qian , Mao Shanjun , Xi Lingling , Wang Yong
Published: July 2023

Electrochemical hydrogen evolution reaction in neutral media is listed as the most difficult challenges of energy catalysis due to the sluggish kinetics. Herein, the Ir-HxWO3 catalyst is readily synthesized and exhibits enhanced performance for neutral hydrogen evolution reaction. HxWO3 support is functioned as proton sponge to create a local acid-like microenvironment around Ir metal sites by spontaneous injection of protons to WO3, as evidenced by spectroscopy and electrochemical analysis. Rationalize revitalized lattice-hydrogen species located in the interface are coupled with Had atoms on metallic Ir surfaces via thermodynamically favorable Volmer-Tafel steps, and thereby a fast kinetics. Elaborated Ir-HxWO3 demonstrates acid-like activity with a low overpotential of 20 mV at 10 mA cm−2 and low Tafel slope of 28 mV dec−1, which are even comparable to those in acidic environment. The concept exemplified in this work offer the possibilities for tailoring local reaction microenvironment to regulate catalytic activity and pathway.
Symmetry-breaking dynamics in a tautomeric 3D covalent organic framework
Authors: Xu Yangyang , Sun Tu , Zeng Tengwu , Zhang Xiangyu , Yao Xuan , Liu Shan , Shi Zhaolin , Wen Wen , Zhao Yingbo , Jiang Shan , Ma Yanhang , Zhang Yue-Biao
Published: July 2023

The enolimine-ketoenamine tautomerism has been utilised to construct 2D covalent organic frameworks (COFs) with a higher level of chemical robustness and superior photoelectronic activity. However, it remains challenging to fully control the tautomeric states and correlate their tautomeric structure-photoelectronic properties due to the mobile equilibrium of proton transfer between two other atoms. We show that symmetry-asymmetry tautomerisation from diiminol to iminol/cis-ketoenamine can be stabilised and switched in a crystalline, porous, and dynamic 3D COF (dynaCOF-301) through concerted structural transformation and host-guest interactions upon removal and adaptive inclusion of various guest molecules. Specifically, the tautomeric dynaCOF-301 is constructed by linking the hydroquinone with a tetrahedral building block through imine linkages to form 7-fold interwoven diamondoid networks with 1D channels. Reversible framework deformation and ordering-disordering transition are determined from solvated to activated and hydrated phases, accompanied by solvatochromic and hydrochromic effects useful for rapid, steady, and visual naked-eye chemosensing.
Oxidative phosphorylation is a metabolic vulnerability of endocrine therapy and palbociclib resistant metastatic breast cancers
Authors: El-Botty Rania , Morriset Ludivine , Montaudon Elodie , Tariq Zakia , Schnitzler Anne , Bacci Marina , Lorito Nicla , Sourd Laura , Huguet Léa , Dahmani Ahmed , Painsec Pierre , Derrien Heloise , Vacher Sophie , Masliah-Planchon Julien , Raynal Virginie , Baulande Sylvain , Larcher Thibaut , Vincent-Salomon Anne , Dutertre Guillaume , Cottu Paul , Gentric Géraldine , Mechta-Grigoriou Fatima , Hutton Scott , Driouch Keltouma , Bièche Ivan , Morandi Andrea , Marangoni Elisabetta
Published: July 2023

Resistance to endocrine treatments and CDK4/6 inhibitors is considered a near-inevitability in most patients with estrogen receptor positive breast cancers (ER + BC). By genomic and metabolomics analyses of patients’ tumours, metastasis-derived patient-derived xenografts (PDX) and isogenic cell lines we demonstrate that a fraction of metastatic ER + BC is highly reliant on oxidative phosphorylation (OXPHOS). Treatment by the OXPHOS inhibitor IACS-010759 strongly inhibits tumour growth in multiple endocrine and palbociclib resistant PDX. Mutations in thePIK3CA/AKT1genes are significantly associated with response to IACS-010759. At the metabolic level, in vivo response to IACS-010759 is associated with decreased levels of metabolites of the glutathione, glycogen and pentose phosphate pathways in treated tumours. In vitro, endocrine and palbociclib resistant cells show increased OXPHOS dependency and increased ROS levels upon IACS-010759 treatment. Finally, in ER + BC patients, high expression of OXPHOS associated genes predict poor prognosis. In conclusion, these results identify OXPHOS as a promising target for treatment resistant ER + BC patients.
Polymeric epitope-based vaccine induces protective immunity against group A Streptococcus
Authors: Chen Shuxiong , Ozberk Victoria , Sam Gayathri , Gonzaga Zennia Jean C. , Calcutt Ainslie , Pandey Manisha , Good Michael F. , Rehm Bernd H. A.
Published: July 2023

Group A Streptococcus (Strep A) is a life-threatening human pathogen with no licensed vaccine. Here, we used a biopolymer particle (BP) approach to display repeats of Strep A vaccine candidate peptides p*17 and K4S2 derived from M and non-M protein, respectively. BPs densely displaying both peptides (BP-p*17-S2) were successfully assembled in one-step inside an engineered endotoxin-free Escherichia coli strain. Purified BP-p*17-S2 showed a spherical core-shell morphology with a biopolymer core and peptide shell. Upon formulation with aluminum hydroxide as adjuvant, BP-p*17-S2 exhibited a mean diameter of 2.9 µm and a positive surface charge of 22 mV. No cytotoxicity was detected when tested against HEK-293 cells. Stability studies showed that BP-p*17-S2 is ambient-temperature stable. Immunized mice showed no adverse reactions, while producing high titers of peptide specific antibodies and cytokines. This immune response could be correlated with protective immunity in an animal model of infection, i.e. intranasal challenge of mice with Strep A, where a significant reduction of >100-fold of pathogen burden in nose-associated lymphoid tissue, lung, and spleen was obtained. The cost-effective scalable manufacture of ambient-temperature stable BPs coated with Strep A peptides combined with their immunogenic properties offer an attractive alternative strategy to current Strep A vaccine development.
Database of lower limb kinematics and electromyography during gait-related activities in able-bodied subjects
Authors: Schulte Robert V. , Prinsen Erik C. , Schaake Leendert , Paassen Robert P. G. , Zondag Marijke , van Staveren Eline S. , Poel Mannes , Buurke Jaap H.
Published: July 2023

This data descriptor describes the Roessingh Research & Development-MyLeg database for activity prediction (MyPredict), containing three data sets. These data sets contain data from 55 able-bodied subjects, mean age 24 ± 2 years, measured in 85 measurement sessions. Measurement sessions consisted of trials containing sitting, standing, overground walking, stair ascent, stair descent, ramp ascent, ramp descent, walking on uneven terrain and walking in simulated confined spaces. Subjects were measured using eight inertial measurement units in combination with different types of sEMG. Recorded kinematics consisted of joint angles, sensor accelerations, angular velocity, orientation and virtual marker positions. sEMG was recorded using bipolar sEMG, multi-array sEMG or a combination of both. All data showed excellent correlation with other online available data sets. The data reported in this descriptor forms a solid basis for research into myoelectric pattern recognition, myoelectric control development and electromyography to be used in data-driven applications.
Correlational study on novelty factor, immersive tendencies, purchase intention and memory in immersive VR e-commerce applications
Authors: Gonçalves Guilherme , Meirinhos Galvão , Melo Miguel , Bessa Maximino
Published: July 2023

E-commerce is a field that changed how consumers purchase and interact with products. Although, inherent limitations such as the difficulty of testing the products “first-hand” before a purchase can compromise consumers’ trust in online purchases. Virtual Reality (VR) has been investigated as a tool to solve limitations in several fields and how we can harness its potential to improve the overall user experience. This study analysed how immersive VR (IVR) could solve these limitations by allowing consumers to test products beforehand. We have studied how the Novelty Factor (evaluated by the users’ past VR experience) and Immersive Tendencies correlate with the users’ Purchase Intention and Memory (how well they remember the product’s characteristics). We have analysed a sample of 38 participants (21 males) from 18 to 28 years old. Participants experienced a refrigerator with an interactive touchscreen in an IVR setup and were guided through its functionalities. Results indicated that memory of the product’s characteristics was positively correlated with how recently they experienced VR. No correlations were found in the female sample. A negative correlation between Purchase Intention and Memory of the product’s characteristics was found in the male sample. We concluded that IVR applications could become helpful for both consumers and online shops in an e-commerce context regardless of the Novelty Factor and Immersive Tendencies of consumers. However, differences between genders should be further investigated.
Integrating local and global information to identify influential nodes in complex networks
Authors: Mukhtar Mohd Fariduddin , Abal Abas Zuraida , Baharuddin Azhari Samsu , Norizan Mohd Natashah , Fakhruddin Wan Farah Wani Wan , Minato Wakisaka , Rasib Amir Hamzah Abdul , Abidin Zaheera Zainal , Rahman Ahmad Fadzli Nizam Abdul , Anuar Siti Haryanti Hairol
Published: July 2023

Centrality analysis is a crucial tool for understanding the role of nodes in a network, but it is unclear how different centrality measures provide much unique information. To improve the identification of influential nodes in a network, we propose a new method called Hybrid-GSM (H-GSM) that combines the K-shell decomposition approach and Degree Centrality. H-GSM characterizes the impact of nodes more precisely than the Global Structure Model (GSM), which cannot distinguish the importance of each node. We evaluate the performance of H-GSM using the SIR model to simulate the propagation process of six real-world networks. Our method outperforms other approaches regarding computational complexity, node discrimination, and accuracy. Our findings demonstrate the proposed H-GSM as an effective method for identifying influential nodes in complex networks.
Craniectomy size and decompression of the temporal base using the altered posterior question-mark incision for decompressive hemicraniectomy
Authors: Früh A. , Zdunczyk A. , Wolf S. , Mertens R. , Spindler P. , Wasilewski D. , Hecht N. , Bayerl S. , Onken J. , Wessels L. , Faust K. , Vajkoczy P. , Truckenmueller P.
Published: July 2023

The altered posterior question-mark incision for decompressive hemicraniectomy (DHC) was proposed to reduce the risk of intraoperative injury of the superficial temporal artery (STA) and demonstrated a reduced rate of wound-healing disorders after cranioplasty. However, decompression size during DHC is essential and it remains unclear if the new incision type allows for an equally effective decompression. Therefore, this study evaluated the efficacy of the altered posterior question-mark incision for craniectomy size and decompression of the temporal base and assessed intraoperative complications compared to a modified standard reversed question-mark incision. The authors retrospectively identified 69 patients who underwent DHC from 2019 to 2022. Decompression and preservation of the STA was assessed on postoperative CT scans and CT or MR angiography. Forty-two patients underwent DHC with the standard reversed and 27 patients with the altered posterior question-mark incision. The distance of the margin of the craniectomy to the temporal base was 6.9 mm in the modified standard reversed and 7.2 mm in the altered posterior question-mark group (p = 0.77). There was no difference between the craniectomy sizes of 158.8 mm and 158.2 mm, respectively (p = 0.45), and there was no difference in the rate of accidental opening of the mastoid air cells. In both groups, no transverse/sigmoid sinus was injured. Twenty-four out of 42 patients in the modified standard and 22/27 patients in the altered posterior question-mark group had a postoperative angiography, and the STA was preserved in all cases in both groups. Twelve (29%) and 5 (19%) patients underwent revision due to wound-healing disorders after DHC, respectively (p = 0.34). There was no difference in duration of surgery. Thus, the altered posterior question-mark incision demonstrated technically equivalent and allows for an equally effective craniectomy size and decompression of the temporal base without increasing risks of intraoperative complications. Previously described reduction in wound-healing complications and cranioplasty failures needs to be confirmed in prospective studies to demonstrate the superiority of the altered posterior question-mark incision.
Epidemic thresholds and human mobility
Authors: Pardo-Araujo Marta , García-García David , Alonso David , Bartumeus Frederic
Published: July 2023

A comprehensive view of disease epidemics demands a deep understanding of the complex interplay between human behaviour and infectious diseases. Here, we propose a flexible modelling framework that brings conclusions about the influence of human mobility and disease transmission on early epidemic growth, with applicability in outbreak preparedness. We use random matrix theory to compute an epidemic threshold, equivalent to the basic reproduction number $$R_{0}$$

R
0

, for a SIR metapopulation model. The model includes both systematic and random features of human mobility. Variations in disease transmission rates, mobility modes (i.e. commuting and migration), and connectivity strengths determine the threshold value and whether or not a disease may potentially establish in the population, as well as the local incidence distribution.
State-of-the-art predictive modeling of heavy metal ions removal from the water environment using nanotubes
Authors: Ghasemi Zeinab , Farzad Farzaneh , Zaboli Ameneh , Zeraatkar Moghaddam Ali
Published: July 2023

In this research, molecular dynamics (MD) simulation is used to investigate the efficiency of carbon nanotubes (CNT) and boron nitride nanotubes (BNNT) in removing lead ions from contaminated waters. Then the effect of functionalizing nanotubes with –COO– and COOH– functional groups and the nanotubes’ absorption performance of two different concentrations of lead ions are studied. To better evaluate adsorption process, the set of descriptors, such as interaction energies, radial distribution function, etc., are calculated. The MD results show that the absorption performance is significantly improved by modifying the surface of CNT and BNNT with functional groups. In addition, the adsorption capacity increases in higher concentrations of Pb ions at BNNTCOO– and CNTCOOH systems. The interaction energy of BNNTCOO– with a concentration of 50 lead ions is − 2879.28 kJ/mol, which is about 106 kJ/mol more negative than BNNTCOO– at a concentration of 20 lead ions. Also, it is observed that the functionalization of both nanotubes with –COO– increases their absorption capacity. The obtained results from this study provide significant information about the mechanisms of lead adsorption on the surface of nanotubes.
Polymer based dual drug delivery system for targeted treatment of fluoroquinolone resistant Staphylococcus aureus mediated infections
Authors: Thamilselvan Gopalakrishnan , David Helma , Sajeevan Anusree , Rajaramon Shobana , Solomon Adline Princy , Durai Ramya Devi , Narayanan Vedha Hari B.
Published: July 2023

The present study attempts to treat S. aureus-induced soft skin infections using a combinatorial therapy with an antibiotic, Ciprofloxacin (CIP), and an efflux pump inhibitor 5-Nitro-2-(3-phenylpropoxy) pyridine (5-NPPP) through a smart hydrogel delivery system. The study aims to reduce the increasing rates of infections and antimicrobial resistance; therefore, an efflux pump inhibitor molecule is synthesized and delivered along with an antibiotic to re-sensitize the pathogen towards antibiotics and treat the infections. CIP-loaded polyvinyl alcohol (PVA) hydrogels at varying concentrations were fabricated and optimized by a chemical cross-linking process, which exhibited sustained drug release for 5 days. The compound 5-NPPP loaded hydrogels provided linear drug release for 2 days, necessitating the need for the development of polymeric nanoparticles to alter the release drug pattern. 5-NPPP loaded Eudragit RSPO nanoparticles were prepared by modified nanoprecipitation—solvent evaporation method, which showed optimum average particle size of 230–280 nm with > 90% drug entrapment efficiency. The 5-NPPP polymeric nanoparticles loaded PVA hydrogels were fabricated to provide a predetermined sustained release of the compound to provide a synergistic effect. The selected 7% PVA hydrogels loaded with the dual drugs were evaluated using Balb/c mice models induced with S. aureus soft skin infections. The results of in vivo studies were evidence that the dual drugs loaded hydrogels were non-toxic and reduced the bacterial load causing re-sensitization towards antibiotics, which could initiate re-epithelization. The research concluded that the PVA hydrogels loaded with CIP and 5-NPPP nanoparticles could be an ideal and promising drug delivery system for treating S. aureus-induced skin infections.
Longitudinal in vivo metabolic labeling reveals tissue-specific mitochondrial proteome turnover rates and proteins selectively altered by parkin deficiency
Authors: Stauch K. L. , Totusek S. , Trease A. J. , Estrella L. D. , Emanuel K. , Fangmeier A. , Fox H. S.
Published: July 2023

Our study utilizes a longitudinal isotopic metabolic labeling approach in vivo in combination with organelle fraction proteomics to address the role of parkin in mitochondrial protein turnover in mice. The use of metabolic labeling provides a method to quantitatively determine the global changes in protein half-lives whilst simultaneously assessing protein expression. Studying two diverse mitochondrial populations, we demonstrated the median half-life of brain striatal synaptic mitochondrial proteins is significantly greater than that of hepatic mitochondrial proteins (25.7 vs. 3.5 days). Furthermore, loss of parkin resulted in an overall, albeit modest, increase in both mitochondrial protein abundance and half-life. Pathway and functional analysis of our proteomics data identified both known and novel pathways affected by loss of parkin that are consistent with its role in both mitochondrial quality control and neurodegeneration. Our study therefore adds to a growing body of evidence suggesting dependence on parkin is low for basal mitophagy in vivo and provides a foundation for the investigation of novel parkin targets.
Accurate detection of paroxysmal atrial fibrillation with certified-GAN and neural architecture search
Authors: Asadi Mehdi , Poursalim Fatemeh , Loni Mohammad , Daneshtalab Masoud , Sjödin Mikael , Gharehbaghi Arash
Published: July 2023

This paper presents a novel machine learning framework for detecting PxAF, a pathological characteristic of electrocardiogram (ECG) that can lead to fatal conditions such as heart attack. To enhance the learning process, the framework involves a generative adversarial network (GAN) along with a neural architecture search (NAS) in the data preparation and classifier optimization phases. The GAN is innovatively invoked to overcome the class imbalance of the training data by producing the synthetic ECG for PxAF class in a certified manner. The effect of the certified GAN is statistically validated. Instead of using a general-purpose classifier, the NAS automatically designs a highly accurate convolutional neural network architecture customized for the PxAF classification task. Experimental results show that the accuracy of the proposed framework exhibits a high value of 99.0% which not only enhances state-of-the-art by up to 5.1%, but also improves the classification performance of the two widely-accepted baseline methods, ResNet-18, and Auto-Sklearn, by $$2.2\%$$

2.2
%

and $$6.1\%$$

6.1
%

.