bluuebunny/crossref_metadata_2025 huggingface dataset:
Visceral Adiposity in Relation to Body Adiposity and Nutritional Status in Elderly Patients with Stable Coronary Artery Disease
Authors: Hudzik Bartosz , Nowak Justyna , Szkodziński Janusz , Zubelewicz-Szkodzińska Barbara
Published: July 2021

Introduction: The accumulation of visceral abdominal tissue (VAT) seems to be a hallmark feature of abdominal obesity and substantially contributes to metabolic abnormalities. There are numerous factors that make the body-mass index (BMI) a suboptimal measure of adiposity. The visceral adiposity index (VAI) may be considered a simple surrogate marker of visceral adipose tissue dysfunction. However, the evidence comparing general to visceral adiposity in CAD is scarce. Therefore, we have set out to investigate visceral adiposity in relation to general adiposity in patients with stable CAD. Material and methods: A total of 204 patients with stable CAD hospitalized in the Department of Medicine and the Department of Geriatrics entered the study. Based on the VAI-defined adipose tissue dysfunction (ATD) types, the study population (N = 204) was divided into four groups: (1) no ATD (N = 66), (2) mild ATD (N = 50), (3) moderate ATD (N = 48), and (4) severe ATD (N = 40). Nutritional status was assessed using the Controlling Nutritional Status (CONUT) score. Results: Patients with moderate and severe ATD were the youngest (median 67 years), yet their metabolic age was the oldest (median 80 and 84 years, respectively). CONUT scores were similar across all four study groups. The VAI had only a modest positive correlation with BMI (r = 0.59 p < 0.01) and body adiposity index (BAI) (r = 0.40 p < 0.01). There was no correlation between VAI and CONUT scores. There was high variability in the distribution of BMI-defined weight categories across all four types of ATD. A total of 75% of patients with normal nutritional status had some form of ATD, and one-third of patients with moderate or severe malnutrition did not have any ATD (p = 0.008). In contrast, 55–60% of patients with mild, moderate, or severe ATD had normal nutritional status (p = 0.008). ROC analysis demonstrated that BMI and BAI have poor predictive value in determining no ATD. Both BMI (AUC 0.78 p < 0.0001) and BAI (AUC 0.66 p = 0.003) had strong predictive value for determining severe ATD (the difference between AUC 0.12 being p = 0.0002). However, BMI predicted mild ATD and severe ATD better than BAI. Conclusions: ATD and malnutrition were common in patients with CAD. Notably, this study has shown a high rate of misclassification of visceral ATD via BMI and BAI. In addition, we demonstrated that the majority of patients with normal nutritional status had some form of ATD and as much as one-third of patients with moderate or severe malnutrition did not have any ATD. These findings have important clinical ramifications for everyday practice regarding the line between health and disease in the context of malnutrition in terms of body composition and visceral ATD, which are significant for developing an accurate definition of the standards for the intensity of clinical interventions.
Utilizing Participatory Research to Engage Underserved Populations to Improve Health-Related Outcomes in Delaware
Authors: Robson Shannon M. , Rex Samantha M. , Greenawalt Katie , Peterson P. Michael , Orsega-Smith Elizabeth
Published: July 2021

Cooperative Extension is a community outreach program. Despite its large reach, there is a need for the evaluation of changes in health-related outcomes for individuals engaged with Cooperative Extension. A team-based challenge was developed using community-engaged participatory research integrated with Cooperative Extension to encourage healthy eating and physical activity behaviors through Cooperative Extension programming. Thus, the primary purpose of this secondary analysis was to (1) evaluate changes in anthropometric outcomes and (2) evaluate changes in health behavior outcomes. Associations of anthropometric changes and health behavior changes with engagement in the three-month team-based challenge were explored. Anthropometrics were measured using standard procedures, and intake of fruits and vegetables and physical activity were self-reported. Of the 145 participants in the community-engaged participatory research portion of the study, 52.4% (n = 76) had complete anthropometrics before and after the team-based challenge and were included in this study. At 3 months, there was a significant reduction in body mass index (−0.3 kg/m2, p = 0.024) and no significant change in waist circumference (p = 0.781). Fruit and vegetable intake significantly increased (+0.44 servings/day, p = 0.018). Physical activity did not significantly change based on (1) the number of days 30 or more minutes of physical activity was conducted (p = 0.765) and (2) Godin Leisure-Time Exercise Questionnaire scores (p = 0.612). Changes in anthropometrics and health behaviors were not associated with engagement in the team-based challenge. Using community-engaged participatory research with community outreach programs, such as Cooperative Extension, can improve health-related outcomes in underserved populations. However, despite a participatory approach, changes in anthropometrics and health behaviors were not associated with engagement in the developed team-based challenge.
Complementary Foods and Milk-Based Formulas Provide Excess Protein but Suboptimal Key Micronutrients and Essential Fatty Acids in the Intakes of Infants and Toddlers from Urban Settings in Malaysia
Authors: Khor Geok Lin , Lee Siew Siew
Published: July 2021

This study determined the intakes of complementary foods (CFs) and milk-based formulas (MFs) by a total of 119 subjects aged 6–23.9 months from urban day care centers. Dietary intakes were assessed using two-day weighed food records. Intake adequacy of energy and nutrients was compared to the Recommended Nutrient Intakes (RNI) for Malaysia. The most commonly consumed CFs were cereals (rice, noodles, bread). The subjects derived approximately half of their energy requirements (kcals) from CFs (57 ± 35%) and MFs (56 ± 31%). Protein intake was in excess of their RNI requirements, from both CFs (145 ± 72%) and MFs (133 ± 88%). Main sources of protein included meat, dairy products, and western fast food. Intake of CFs provided less than the RNI requirements for vitamin A, thiamine, riboflavin, folate, vitamin C, calcium, iron, and zinc. Neither CF nor MF intake met the Adequate Intake (AI) requirements for essential fatty acids. These findings indicate imbalances in the dietary intake of the subjects that may have adverse health implications, including increased risk of rapid weight gain from excess protein intake, and linear growth faltering and intellectual impairment from multiple micronutrient deficiencies. Interventions are needed to improve child feeding knowledge and practices among parents and child care providers.
Molecular Diagnosis of Leishmaniasis: Quantification of Parasite Load by a Real-Time PCR Assay with High Sensitivity
Authors: Castelli Germano , Bruno Federica , Reale Stefano , Catanzaro Simone , Valenza Viviana , Vitale Fabrizio
Published: July 2021

Real-time PCR was developed to quantify Leishmania infantum kinetoplast DNA and optimized to achieve a sensitivity of 1 parasite/mL. For this purpose, we cloned the conserved kDNA fragment of 120 bp into competent cells and correlated them with serial dilutions of DNA extracted from reference parasite cultures calculating that a parasite cell contains approximately 36 molecules of kDNA. This assay was applied to estimate parasite load in clinical samples from visceral, cutaneous leishmaniasis patients and infected dogs and cats comparing with conventional diagnosis. The study aimed to propose a real-time PCR for the detection of Leishmania DNA from clinical samples trying to solve the diagnostic problems due to the low sensitivity of microscopic examination or the low predictive values of serology and resolve problems related to in vitro culture. The quantitative PCR assay in this study allowed detection of Leishmania DNA and quantification of considerably low parasite loads in samples that had been diagnosed negative by conventional techniques. In conclusion, this quantitative PCR can be used for the diagnosis of both human, canine and feline Leishmaniasis with high sensitivity and specificity, but also for evaluating treatment and the endpoint determination of leishmaniasis.
The m15 Locus of Murine Cytomegalovirus Modulates Natural Killer Cell Responses to Promote Dissemination to the Salivary Glands and Viral Shedding
Authors: Chan Baca , Arapović Maja , Masters Laura L. , Rwandamuiye Francois , Jonjić Stipan , Smith Lee M. , Redwood Alec J.
Published: July 2021

As the largest herpesviruses, the 230 kb genomes of cytomegaloviruses (CMVs) have increased our understanding of host immunity and viral escape mechanisms, although many of the annotated genes remain as yet uncharacterised. Here we identify the m15 locus of murine CMV (MCMV) as a viral modulator of natural killer (NK) cell immunity. We show that, rather than discrete transcripts from the m14, m15 and m16 genes as annotated, there are five 3′-coterminal transcripts expressed over this region, all utilising a consensus polyA tail at the end of the m16 gene. Functional inactivation of any one of these genes had no measurable impact on viral replication. However, disruption of all five transcripts led to significantly attenuated dissemination to, and replication in, the salivary glands of multiple strains of mice, but normal growth during acute infection. Disruption of the m15 locus was associated with heightened NK cell responses, including enhanced proliferation and IFNγ production. Depletion of NK cells, but not T cells, rescued salivary gland replication and viral shedding. These data demonstrate the identification of multiple transcripts expressed by a single locus which modulate, perhaps in a concerted fashion, the function of anti-viral NK cells.
Bacteriocin Production by Beta-Hemolytic Streptococci
Authors: Vogel Verena , Spellerberg Barbara
Published: July 2021

Beta-hemolytic streptococci cause a variety of infectious diseases associated with high morbidity and mortality. A key factor for successful infection is host colonization, which can be difficult in a multispecies environment. Secreting bacteriocins can be beneficial during this process. Bacteriocins are small, ribosomally produced, antimicrobial peptides produced by bacteria to inhibit the growth of other, typically closely related, bacteria. In this systematic review, bacteriocin production and regulation of beta-hemolytic streptococci was surveyed. While Streptococcus pyogenes produces eight different bacteriocins (Streptococcin A-FF22/A-M49, Streptin, Salivaricin A, SpbMN, Blp1, Blp2, Streptococcin A-M57), only one bacteriocin of Streptococcus agalactiae (Agalacticin = Nisin P) and one of Streptococcus dysgalactiae subsp. equisimilis (Dysgalacticin) has been described. Expression of class I bacteriocins is regulated by a two-component system, typically with autoinduction by the bacteriocin itself. In contrast, a separate quorum sensing system regulates expression of class II bacteriocins. Both identified class III bacteriocins are plasmid-encoded and regulation has not been elucidated.
Merging the Multi-Target Effects of Kleeb Bua Daeng, a Thai Traditional Herbal Formula in Unpredictable Chronic Mild Stress-Induced Depression
Authors: Maneenet Juthamart , Monthakantirat Orawan , Daodee Supawadee , Boonyarat Chantana , Chotritthirong Yutthana , Kwankhao Pakakrong , Pitiporn Supaporn , Awale Suresh , Chulikhit Yaowared
Published: July 2021

Major depressive disorder (MDD) is a common and debilitating psychiatric disease characterized by persistent low mood, lack of energy, hypoactivity, anhedonia, decreased libido, and impaired cognitive and social functions. However, the multifactorial etiology of MDD remains largely unknown due the complex interaction between genetics and environment involved. Kleeb Bua Daeng (KBD) is a Thai traditional herbal formula that has been used to promote brain health. It consists of a 1:1:1 ratio of the aerial part of Centella asiatica, Piper nigrum fruit, and the petals of Nelumbo nucifera. According to the pharmacological activities of the individual medicinal plants, KBD has good potential as a treatment for MDD. The present study investigated the antidepressant activity of KBD in an unpredictable chronic mild stress (UCMS) mouse model. Daily administration of KBD to UCMS mice ameliorated both anhedonia, by increasing 2% sucrose intake, and hopeless behavior, by reducing immobility times in the forced swimming test (FST) and tail suspension test (TST) without any effect on locomotor activity. The mechanism of KBD activity was multi-modal. KBD promoted neurogenesis by upregulation of brain-derived neurotrophic factor (BDNF) and cyclic AMP-responsive element binding (CREB) mRNA expression in the frontal cortex and hippocampus. Daily treatment with KBD significantly reversed UCMS-induced HPA axis dysregulation by upregulating the glucocorticoid receptor (GR) while downregulating serum- and glucocorticoid-inducible kinase 1 (SGK1) and FK506 binding protein 5 (FKBP5) mRNA expression. KBD treatment also normalized proinflammatory cytokine expression including tumor necrosis factor-alpha (TNF-α), and interleukin (IL)-1β and IL-6. KBD and its component extracts also exhibited an inhibitory effect in vitro on monoamine oxidase (MAO) A and B. The multiple antidepressant actions of KBD emphasize its potential as an effective, novel treatment for MDD.
Cancer Mechanisms and Emerging Therapies
Authors: Gulei Diana , Indini Alice , Jerónimo Carmen , Iuga Cristina-Adela , Grossi Francesco
Published: July 2021

Over the last decades, cancer has become one of the most relevant health issues at a worldwide level [...]
Optimized Taste-Masked Microparticles for Orally Disintegrating Tablets as a Promising Dosage Form for Alzheimer’s Disease Patients
Authors: Sutthapitaksakul Lalinthip , Thanawuth Kasitpong , Dass Crispin R. , Sriamornsak Pornsak
Published: July 2021

The objective of this research was to optimize the tasted-masked microparticles for orally disintegrating tablets containing donepezil hydrochloride using quality risk assessment and design of experiment approaches. The double emulsion solvent evaporation technique using aminoalkyl methacrylate copolymer (AMC) was used to prepare taste-masked microparticles. Factors affecting the quality of the taste-masked microparticles were analyzed using an Ishikawa diagram. A risk-ranking approach was used to rank the formulation and process risks. Furthermore, the effect of AMC quantity, stirring time, and volume of outer water phase on various responses, such as particle size, the amount of drug dissolved at 5 min (Q5) in simulated saliva fluid, and mean dissolution time (MDT) in simulated gastric fluid, was investigated using the Box-Behnken design. The optimized microparticles were then used to prepare orally disintegrating tablets (ODTs) and evaluated by in vitro and in vivo testing. The results demonstrated that particle size was influenced by the AMC amount and stirring time. Q5 was significantly affected by the amount of AMC and the volume of the outer water phase. On the other hand, these two factors had a positive effect on MDT. The optimized microparticles had a particle size of 174.45 ± 18.19 µm, Q5 of 5.04%, and MDT of 5.97 min. The ODTs with taste-masked microparticles showed acceptable in vitro dissolution with an MDT of 5 min. According to the results of a panel of six human volunteers, they greatly improved palatability.
Sustained In Vitro and In Vivo Delivery of Metformin from Plant Pollen-Derived Composite Microcapsules
Authors: Meligi Noha M. , Dyab Amro K. F. , Paunov Vesselin N.
Published: July 2021

We developed a dual microencapsulation platform for the type 2 diabetes drug metformin (MTF), which is aimed to increase its bioavailability. We report the use of Lycopodium clavatum sporopollenin (LCS), derived from their natural spores, and raw Phoenix dactylifera L. (date palm) pollens (DPP) for MTF microencapsulation. MTF was loaded into LCS and DPP via a vacuum and a novel method of hydration-induced swelling. The loading capacity (LC) and encapsulation efficiency (EE) percentages for MTF-loaded LCS and MTF-loaded DPP microcapsules were 14.9% ± 0.7, 29.8 ± 0.8, and 15.2% ± 0.7, 30.3 ± 1.0, respectively. The release of MTF from MTF-loaded LCS microcapsules was additionally controlled by re-encapsulating the loaded microcapsules into calcium alginate (ALG) microbeads via ionotropic gelation, where the release of MTF was found to be significantly slower and pH-dependent. The pharmacokinetic parameters, obtained from the in vivo study, revealed that the relative bioavailability of the MTF-loaded LCS-ALG beads was 1.215 times higher compared to pure MTF, following oral administration of a single dose equivalent to 25 mg/kg body weight MTF to streptozotocin (STZ)-induced diabetic male Sprague-Dawley rats. Significant hypoglycemic effect was obtained for STZ-induced diabetic rats orally treated with MTF-loaded LCS-ALG beads compared to control diabetic rats. Over a period of 29 days, the STZ-induced diabetic rats treated with MTF-loaded LCS-ALG beads showed a decrease in the aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides, cholesterol, and low-density lipoprotein-cholesterol (LDL-C) levels, as well as an increase in glutathione peroxidase (GPx) and a recovery in the oxidative stress biomarker, lipid peroxidation (LPx). In addition, histopathological studies of liver, pancreas, kidney, and testes suggested that MTF-loaded LCS-ALG beads improved the degenerative changes in organs of diabetic rats. The LCS-ALG platform for dual encapsulation of MTF achieved sustained MTF delivery and enhancement of bioavailability, as well as the improved biochemical and histopathological characteristics in in vivo studies, opening many other intriguing applications in sustained drug delivery.
Liqui-Mass Technology as a Novel Tool to Produce Sustained Release Liqui-Tablet Made from Liqui-Pellets
Authors: Lam Matthew , Nashed Nour , Nokhodchi Ali
Published: July 2021

The Liqui-Mass technology (also known as Liqui-Pellet technology) has shown promising results in terms of enhancing the drug release rate of water insoluble drugs in a simplistic approach. However, there is no current study on sustained-release formulation using the Liqui-Mass technology. In this study, an attempt was made to produce a sustained-release Liqui-Tablet for the first time using a matrix-based approach. The non-volatile co-solvent used in the investigation included Tween 80, Tween 20 and Kolliphor EL. The production of sustained-release propranolol hydrochloride Liqui-Tablet was successful, and data from the saturation solubility test and dissolution test did not show much difference among the mentioned non-volatile co-solvent. The best Liqui-Tablet formulation took 24 h for drug release to reach at around 100%. There seemed to be a synergistic retarding drug release effect when a non-volatile co-solvent and Eudragit RS PO were used together. The increase of Eudragit RS PO concentration increased the retardant effect. Kinetic drug release analysis suggests that the best formulation followed the Higuchi model. The flowability of pre-compressed Liqui-Tablet pellets had no issues and its size distribution was narrow. Liqui-Tablet was generally robust and most formulations passed the friability test. The study revealed that Liqui-Mass technology can be employed to sustain drug release.
Quantum Optical Aspects of High-Harmonic Generation
Authors: Varró Sándor
Published: July 2021

The interaction of electrons with strong laser fields is usually treated with semiclassical theory, where the laser is represented by an external field. There are analytic solutions for the free electron wave functions, which incorporate the interaction with the laser field exactly, but the joint effect of the atomic binding potential presents an obstacle for the analysis. Moreover, the radiation is a dynamical system, the number of photons changes during the interactions. Thus, it is legitimate to ask how can one treat the high order processes nonperturbatively, in such a way that the electron-atom interaction and the quantized nature of radiation be simultaneously taken into account? An analytic method is proposed to answer this question in the framework of nonrelativistic quantum electrodynamics. As an application, a quantum optical generalization of the strong-field Kramers-Heisenberg formula is derived for describing high-harmonic generation. Our formalism is suitable to analyse, among various quantal effects, the possible role of arbitrary photon statistics of the incoming field. The present paper is dedicated to the memory of Prof. Dr. Fritz Ehlotzky, who had significantly contributed to the theory of strong-field phenomena over many decades.
High Doses of Essential Oil of Croton Zehntneri Induces Renal Tubular Damage
Authors: Silva Katarine F. , Peruchetti Diogo B. , Sirtoli Gabriela M. , Takiya Christina M. , Pinheiro Ana Acacia S. , Leal-Cardoso José Henrique , Caruso-Neves Celso
Published: July 2021

The essential oil of Croton zehntneri (EOCZ) and its major compounds are known to have several biological activities. However, some evidence shows potential toxic effects of high doses of EOCZ (>300 mg/kg) in amphibian and human kidneys. The aim of the present work was to investigate the effects on renal function of EOCZ at 300 mg/kg/day in healthy Swiss mice and a subclinical acute kidney injury (subAKI) animal model, which presents tubule-interstitial injury (TII). Four experimental groups were generated: (1) CONT group (control); (2) EOCZ, mice treated with EOCZ; (3) subAKI; (4) subAKI+EOCZ, subAKI treated simultaneously with EOCZ. EOCZ treatment induced TII measured by increases in (1) proteinuria; (2) cortical tubule-interstitial space; (3) macrophage infiltration; (4) collagen deposition. A decrease in tubular sodium reabsorption was also observed. These results were similar and nonadditive to those observed in the subAKI group. These data suggest that treatment with EOCZ at higher concentrations induces TII in mice, which could be mediated by protein overload in the proximal tubule.
DNA Methylation—An Epigenetic Mark in Mutagen-Treated Brachypodium distachyon Cells
Authors: Bara Adrianna Wiktoria , Braszewska Agnieszka , Kwasniewska Jolanta
Published: July 2021

The chromatin structure is significantly influenced by some epigenetic modifications including DNA methylation. The nuclear organization plays an essential role in the cell response to external stresses including mutagens. We present an analysis of the correlation between epigenetic modifications and the instability of the Brachypodium distachyon genome, which are observed as micronuclei, following maleic hydrazide (MH) and nitroso-N-methylurea (MNU) treatments. We compared the level of DNA methylation in the control (untreated) and mutagen-treated B. distachyon nuclei. An immunostaining method using specific antibodies against modified DNA anti-5-methylcytosine was used for the evaluation of DNA methylation in a single nucleus and micronucleus. Interestingly, we showed an alteration of DNA methylation in cells after mutagenic treatments. The results indicate that DNA methylation might be involved in the response of the B. distachyon genome to mutagenic treatments. This demonstrates that analyses of the epigenetic modifications should be integrated into current plant genetic toxicology in order to explain the mechanisms of DNA damage and repair in plants.
Temporal Evolution of the Gravitaxis of Euglena gracilis from a Single Cell
Authors: Ozasa Kazunari , Kang Hyunwoong , Song Simon , Kato Shota , Shinomura Tomoko , Maeda Mizuo
Published: July 2021

Gravitaxis is one of the most important issues in the growth of microalgae in the water column; it determines how easily cells receive sunlight with a comfortable intensity that is below the damaging threshold. We quantitatively investigated and analyzed the gravitaxis and cell multiplication of Euglena gracilis using vertically placed microchambers containing a single cell. A temporal change in gravitaxis and cell multiplication was observed after transferring the cells to fresh culture medium for 9 days. We performed 29 individual experiments with 2.5 mm × 2.5 mm × 0.1 mm square microchambers and found that the cells showed positive, negative, and moderate gravitaxis in 8, 7, and 14 cases, respectively, after transferring to fresh culture medium. A common trend was observed for the temporal change in gravitaxis for the eight initially positive gravitaxis cases. The cells with initially positive gravitaxis showed a higher rate of cell multiplication than those with initially negative gravitaxis. We also discussed the gravitaxis mechanism of E. gracilis from the observed trend of gravitaxis change and swimming traces. In addition, bioconvection in a larger and thicker chamber was investigated at a millimeter scale and visualized.
Naturally Occurring Polyelectrolytes and Their Use for the Development of Complex-Based Mucoadhesive Drug Delivery Systems: An Overview
Authors: Cazorla-Luna Raúl , Martín-Illana Araceli , Notario-Pérez Fernando , Ruiz-Caro Roberto , Veiga María-Dolores
Published: July 2021

Biopolymers have several advantages for the development of drug delivery systems, since they are biocompatible, biodegradable and easy to obtain from renewable resources. However, their most notable advantage may be their ability to adhere to biological tissues. Many of these biopolymers have ionized forms, known as polyelectrolytes. When combined, polyelectrolytes with opposite charges spontaneously form polyelectrolyte complexes or multilayers, which have great functional versatility. Although only one natural polycation—chitosan has been widely explored until now, it has been combined with many natural polyanions such as pectin, alginate and xanthan gum, among others. These polyelectrolyte complexes have been used to develop multiple mucoadhesive dosage forms such as hydrogels, tablets, microparticles, and films, which have demonstrated extraordinary potential to administer drugs by the ocular, nasal, buccal, oral, and vaginal routes, improving both local and systemic treatments. The advantages observed for these formulations include the increased bioavailability or residence time of the formulation in the administration zone, and the avoidance of invasive administration routes, leading to greater therapeutic compliance.
Review on Interfacial Bonding Mechanism of Functional Polymer Coating on Glass in Atomistic Modeling Perspective
Authors: Park Hyunhang , Lee Sung Hoon
Published: July 2021

Atomistic modeling methods are successfully applied to understand interfacial interaction in nanoscale size and analyze adhesion mechanism in the organic–inorganic interface. In this paper, we review recent representative atomistic simulation works, focusing on the interfacial bonding, adhesion strength, and failure behavior between polymer film and silicate glass. The simulation works are described under two categories, namely non-bonded and bonded interaction. In the works for non-bonded interaction, three main interactions, namely van der Waals interaction, polar interaction, and hydrogen bonds, are investigated, and the contributions to interfacial adhesion energy are analyzed. It is revealed that the most dominant interaction for adhesion is hydrogen bonding, but flexibility of the polymer film and modes of adhesion measurement test do affect adhesion and failure behavior. In the case of bonded interactions, the mechanism of covalent silane bond formation through condensation and hydrolysis process is reviewed, and surface reactivity, molecular density, and adhesion properties are calculated with an example of silane functionalized polymer. Besides interfacial interactions, effects of external conditions, such as surface morphology of the glass substrate and relative humidity on the adhesion and failure behavior, are presented, and modeling techniques developed for building interfacial system and calculating adhesion strengths are briefly introduced.
Enhancing Anti-Static Performance of Fibers by Construction of the Hybrid Conductive Network Structure on the Fiber Surface
Authors: Xu Congcong , Fang Lin , Yu Mingming , Ren Musu , Sun Jinliang , Zhang Liying
Published: July 2021

The hybrid antistatic agent SCNTs/OAA composed of sulfonated carbon nanotubes (SCNTs) and organic antistatic agent (OAA) was treated on the fiber surface to construct the hybrid conductive layer. Among them, SCNTs were synthesized through a simple method, and their chemical structure and morphology were characterized. SCNTs had good dispersibility due to the presence of sulfonic acid groups, which made SCNTs uniformly dispersed on the fiber surface. The SCNTs/OAA-treated fiber was hardly affected by relative humidity, because SCNTs form a continuous and uniform physical conductive network on the fiber surface. When the addition amount of SCNTs/OAA was 0.5~2 wt%, the fiber had excellent antistatic ability. Under the synergistic effect of SCNTs and OAA, the resistivity of SCNTs/OAA-treated fiber was almost not affected by fiber stretching.
3D Modelling of Mass Transfer into Bio-Composite
Authors: Kabbej Marouane , Guillard Valérie , Angellier-Coussy Hélène , Wolf Caroline , Gontard Nathalie , Gaucel Sébastien
Published: July 2021

A three-dimensional model structure that allows considering interphase layer around permeable inclusions is developed to predict water vapor permeability in composite materials made of a matrix Poly(3-HydroxyButyrate-co-3-HydroxyValerate) (PHBV) including Wheat Straw Fiber (WSF) particles. About 500 two-phase structures corresponding to composites of different particles volume fractions (5.14−11.4−19.52 % v/v) generated using experimental particles’ size distribution have permitted to capture all the variability of the experimental material. These structures have served as a basis to create three-phase structures including interphase zone of altered polymer property surrounding each particle. Finite Element Method (FEM) applied on these structures has permitted to calculate the relative permeability (ratio between composite and neat matrix permeability P/Pm). The numerical results of the two-phase model are consistent with the experimental data for volume fraction lower than 11.4 %v/v but the large upturn of the experimental relative permeability for highest volume fraction is not well represented by the two-phase model. Among hypothesis made to explain model’s deviation, the presence of an interphase with its own transfer properties is numerically tested: numerical exploration made with the three-phase model proves that an interphase of 5 µm thick, with diffusivity of Di≥1×10−10 m2·s−1, would explain the large upturn of permeability at high volume fraction.
Biophysico-Chemical Properties of Alginate Oligomers Obtained by Acid and Oxidation Depolymerization
Authors: Zimoch-Korzycka Anna , Kulig Dominika , Król-Kilińska Żaneta , Żarowska Barbara , Bobak Łukasz , Jarmoluk Andrzej
Published: July 2021

The aim of the study was to obtain alginate oligosaccharides by using two degradation methods of sodium alginate (SA): with hydrochloric acid (G—guluronate, M—mannuronate and G + M fractions) and hydrogen peroxide (HAS—hydrolyzed SA), in order to assess and compare their biological activity and physico-chemical properties, with an attempt to produce gels from the obtained hydrolysates. The efficiency of each method was determined in order to select the fastest and most efficient process. The ferric ion reducing antioxidant power (FRAP), the ability to scavenge DPPH free radicals, rheological properties, Fourier Transformed Spectroscopy (FTIR) and the microbiological test against Escherichia coli and Staphylococcus aureus were performed. In order to check the functional properties of the obtained oligosaccharides, the texture profile analysis was assessed. The hydrolysis yield of acid SA depolymerization was 28.1% and from hydrogen peroxide SA, depolymerization was 87%. The FTIR analysis confirmed the degradation process by both tested methods in the fingerprint region. The highest ferric reducing antioxidant power was noted for HSA (34.7 µg), and the highest hydroxyl radical scavenging activity was obtained by G fraction (346 µg/Trolox ml). The complete growth inhibition (OD = 0) of alginate hydrolysates was 1%. All tested samples presented pseudoplastic behavior, only HSA presented the ability to form gel.
Preparation of Poly(acrylate)/Poly(diallyldimethylammonium) Coacervates without Small Counterions and Their Phase Behavior upon Salt Addition towards Poly-Ions Segregation
Authors: Queirós Marcos Vinícius Aquino , Loh Watson
Published: July 2021

In this work, we report the phase behavior of polyelectrolyte complex coacervates (PECs) of poly(acrylate) (PA−) and poly(diallyldimethylammonium) (PDADMA+) in the presence of inorganic salts. Titrations of the polyelectrolytes in their acidic and alkaline forms were performed to obtain the coacervates in the absence of their small counterions. This approach was previously applied to the preparation of polymer–surfactant complexes, and we demonstrate that it also succeeded in producing complexes free of small counterions with a low extent of Hofmann elimination. For phase behavior studies, two different molar masses of poly(acrylate) and two different salts were employed over a wide concentration range. It was possible to define the regions at which associative and segregative phase separation take place. The latter one was exploited in more details because the segregation phenomenon in mixtures of oppositely charged polyelectrolytes is scarcely reported. Phase composition analyses showed that there is a strong segregation for both PA− and PDADMA+, who are accompanied by their small counterions. These results demonstrate that the occurrence of poly-ion segregation in these mixtures depends on the anion involved: in this case, it was observed with NaCl, but not with Na2SO4.
Characterization and Aerosolization Performance of HydroxyPropyl-Beta-Cyclodextrin Particles Produced Using Supercritical Assisted Atomization
Authors: Wu Hsien-Tsung , Chuang Yao-Hsiang , Lin Han-Cyuan , Chien Liang-Jung
Published: July 2021

In this study, hydroxypropyl-beta-cyclodextrin (HP-β-CD) particles were produced using supercritical assisted atomization (SAA) with carbon dioxide as the spraying medium or co-solute and aqueous ethanol solution as the solvent. The effects of several key factors on the morphology and size of the HP-β-CD particles were investigated. These factors included the solvent effect, temperatures of the precipitator and saturator, concentration of the HP-β-CD solution, and flow rate ratio of carbon dioxide to the HP-β-CD solution. The conducive conditions for producing fine spherical particles were 54.2% (w/w) aqueous ethanol as the solvent; precipitator and saturator temperatures of 373.2 K and 353.2 K, respectively; a flow rate ratio of carbon dioxide to HP-β-CD solution of 1.8; and low concentrations of HP-β-CD solution. The addition of leucine (LEU) enhanced the aerosol performance of the HP-β-CD particles, and the fine particle fraction (FPF) of the HP-β-CD particles with the addition of 13.0 mass% LEU was 1.8 times higher than that of the HP-β-CD particles without LEU. This study shows that LEU can act as a dispersion enhancer and that HP-β-CD particles produced using SAA can be used as pulmonary drug carriers.
CFD Simulation for Estimating Efficiency of PBCF Installed on a 176K Bulk Carrier under Both POW and Self-Propulsion Conditions
Authors: Kim Dong-Hyun , Park Jong-Chun , Jeon Gyu-Mok , Shin Myung-Soo
Published: July 2021

In this paper, the efficiency of Propeller Boss Cap Fins (PBCF) installed at the bulk carrier was estimated under both Propeller Open Water (POW) and self-propulsion conditions. For this estimation, virtual model-basin tests (resistance, POW, and self-propulsion tests) were conducted through Computational Fluid Dynamics (CFDs) simulation. In the resistance test, the total resistance and the wake distribution according to ship speed were investigated. In the POW test, changes of thrust, torque coefficient, and open water efficiency on the propeller according to PBCF installation were investigated. Finally, the International Towing Tank Conference (ITTC) 1978 method was used to predict the effect of PBCF installation on self-propulsive coefficient and brake horsepower. For analyzing incompressible viscous flow field, the Reynolds-Averaged Navier–Stokes (RANS) equation with SST k-ω turbulence model was calculated using Star-CCM+ 11.06.010-R8. All simulation results were validated by comparing the results of model tests conducted at the Korea Research Institute of Ships and Ocean Engineering (KRISO). Consequently, for the self-propulsion test with the PBCF, a 1.5% reduction of brake horsepower was estimated in the simulation and a 0.5% reduction of the brake horsepower was estimated in the experiment.
Physicochemical Characteristics of Vinegar from Banana Peels and Commercial Vinegars before and after In Vitro Digestion
Authors: Prisacaru Ancuța Elena , Ghinea Cristina , Apostol Laura Carmen , Ropciuc Sorina , Ursachi Vasile Florin
Published: July 2021

Vinegar is a fermented food with a diversity of uses seasoning, salad dressing and flavouring for foods. Since ancient times it is considered a remedy for health and today there are different types of vinegar on the market, and many others are under development. Determination of the physicochemical characteristics of the new types of vinegar is necessary in order to improve them. Therefore, the aim of this paper is to compare the physicochemical characteristics of vinegar obtained from banana peels (with or without boiling peels) at different ages, with those of commercial vinegars. The vinegar from banana peels was obtained and aged in our laboratory, while the commercial vinegars were purchased from a local market. The physicochemical characteristics of all the samples were investigated before and after gastric and intestinal digestion. Inductively coupled plasma mass spectrometry was used to determine the mineral content of the vinegars. Additionally, statistical analysis of the results was performed by applying a one-way analysis of variance. Results showed that vinegar obtained from banana peels is clearer and total dry extract values are lower than those of commercial vinegars. Banana peel vinegars have higher antioxidant activity and total polyphenol content similar to the commercial balsamic vinegars. This study advances the knowledge in the field of vinegar production by using raw agricultural by-products.
Fashion and Faith: Girls and First Holy Communion in Twentieth-Century Ireland (c. 1920–1970)
Authors: Delay Cara
Published: July 2021

With a focus on clothing, bodies, and emotions, this article examines girls’ First Holy Communions in twentieth-century Ireland (c. 1920–1970), demonstrating that Irish girls, even at an early age, embraced opportunities to become both the center of attention and central faith actors in their religious communities through the ritual of Communion. A careful study of First Holy Communion, including clothing, reveals the importance of the ritual. The occasion was indicative of much related to Catholic devotional life from independence through Vatican II, including the intersections of popular religion and consumerism, the feminization of devotion, the centrality of the body in Catholicism, and the role that religion played in forming and maintaining family ties, including cross-generational links. First Communion, and especially the material items that accompanied it, initiated Irish girls into a feminized devotional world managed by women and especially mothers. It taught them that purchasing, hospitality, and gift-giving were central responsibilities of adult Catholic women even as it affirmed the bonds between women family members who helped girls prepare for the occasion.
Empirical Models to Characterize the Structural and Physiochemical Properties of Vacuum Gas Oils with Different Saturate Contents
Authors: Stratiev Dicho S. , Shishkova Ivelina K. , Dinkov Rosen K. , Petrov Ivan P. , Kolev Iliyan V. , Yordanov Dobromir , Sotirov Sotir , Sotirova Evdokia , Atanassova Vassia , Ribagin Simeon , Atanassov Krassimir , Stratiev Danail D. , Nenov Svetoslav , Todorova-Yankova Liliana , Zlatanov Kamen
Published: July 2021

Inter-criteria analysis was employed in VGO samples having a saturate content between 0.8 and 93.1 wt.% to define the statistically significant relations between physicochemical properties, empirical structural models and vacuum gas oil compositional information. The use of a logistic function and employment of a non-linear least squares method along with the aromatic ring index allowed for our newly developed correlation to accurately predict the saturate content of VGOs. The empirical models developed in this study can be used not only for obtaining the valuable structural information necessary to predict the behavior of VGOs in the conversion processes but can also be utilized to detect incorrectly performed SARA analyses. This work confirms the possibility of predicting the contents of VGO compounds from physicochemical properties and empirical models.
A Satellite View of an Intense Snowfall in Madrid (Spain): The Storm ‘Filomena’ in January 2021
Authors: Tapiador Francisco J. , Villalba-Pradas Anahí , Navarro Andrés , Martín Raúl , Merino Andrés , García-Ortega Eduardo , Sánchez José Luis , Kim Kwonil , Lee Gyuwon
Published: July 2021

Evaluating satellite ability in capturing sudden natural disasters such as heavy snowstorms is a topic of societal interest. This paper presents a rapid qualitative analysis of an intense snowfall in Madrid using data from the Global Precipitation Measurement (GPM) mission, specifically the GPM IMERG (Integrated Multi-satellitE Retrievals for GPM) Late Precipitation L3 Half Hourly 0.1° × 0.1° V06 estimates of precipitation (IMERG-Late), and Sentinel-2 imagery. The main research question addressed is the consistency of ground observations, model outputs and satellite data, a topic of major interest for an appropriate and timely societal response to severe weather episodes. Indeed, the choice of the ‘Late’ product over the IMERG ‘Final’ or other GPM datasets was motivated by the availability of data for near real-time response to the storm. Additionally, the 30-min temporal resolution of the product would in principle allow for a detailed analysis of the dynamic processes involved in the snowstorm. Using several complementary data sources, it is shown that optical remote sensing sensors (Sentinel) add value to existing ground data and that is invaluable for rapid response to severe meteorological events such as Filomena. Regarding the GPM precipitation radar, the sampling of the GPM-core satellite was insufficient to provide the IMERG algorithm with enough quality data to correctly represent the actual sequence of precipitation. Without corrections, the total precipitation differs from observations by a factor of two. The difficulties of retrieving precipitation with radiometers over snow-covered surfaces was a major factor for the mismatch. Thus, the calibrated precipitation product did not fully capture the historic storm, and neither did the IR-based element of the IMERG-Late product, which is a neural network merging of microwave and infrared data. It follows that increased temporal resolution of spaceborne microwave sensors and improved retrieval of precipitation from radiometers are critical in order to provide a complete account of these sorts of extreme, significant, short-duration cases. Otherwise, the high-quality, radar and radiometer data feeding the high temporal resolution algorithms simply slip through the grasp of the ascending and descending orbits, leaving little quality data to be interpolated into successive overpasses.
Seismic Interferometry from Correlated Noise Sources
Authors: Ayala-Garcia Daniella , Curtis Andrew , Branicki Michal
Published: July 2021

It is a well-established principle that cross-correlating seismic observations at different receiver locations can yield estimates of band-limited inter-receiver Green’s functions. This principle, known as Green’s function retrieval or seismic interferometry, is a powerful technique that can transform noise into signals which enable remote interrogation and imaging of the Earth’s subsurface. In practice it is often necessary and even desirable to rely on noise already present in the environment. Theory that underpins many applications of ambient noise interferometry assumes that the sources of noise are uncorrelated in time. However, many real-world noise sources such as trains, highway traffic and ocean waves are inherently correlated in space and time, in direct contradiction to the these theoretical foundations. Applying standard interferometric techniques to recordings from correlated energy sources makes the Green’s function liable to estimation errors that so far have not been fully accounted for theoretically nor in practice. We show that these errors are significant for common noise sources, always perturbing or entirely obscuring the phase one wishes to retrieve. Our analysis explains why stacking may reduce the phase errors, but also shows that in commonly encountered circumstances stacking will not remediate the problem. This analytical insight allowed us to develop a novel workflow that significantly mitigates effects arising from the use of correlated noise sources. Our methodology can be used in conjunction with already existing approaches, and improves results from both correlated and uncorrelated ambient noise. Hence, we expect it to be widely applicable in ambient noise studies.
Mapping Potato Plant Density Variation Using Aerial Imagery and Deep Learning Techniques for Precision Agriculture
Authors: Mhango Joseph K. , Harris Edwin W. , Green Richard , Monaghan James M.
Published: July 2021

In potato (Solanum tuberosum) production, the number of tubers harvested and their sizes are related to the plant population. Field maps of the spatial variation in plant density can therefore provide a decision support tool for spatially variable harvest timing to optimize tuber sizes by allowing densely populated management zones more tuber-bulking time. Computer vision has been proposed to enumerate plant numbers using images from unmanned aerial vehicles (UAV) but inaccurate predictions in images of merged canopies remains a challenge. Some research has been done on individual potato plant bounding box prediction but there is currently no information on the spatial structure of plant density that these models may reveal and its relationship with potato yield quality attributes. In this study, the Faster Region-based Convolutional Neural Network (FRCNN) framework was used to produce a plant detection model and estimate plant densities across a UAV orthomosaic. Using aerial images of 2 mm ground sampling distance (GSD) collected from potatoes at 40 days after planting, the FRCNN model was trained to an average precision (aP) of 0.78 on unseen testing data. The model was then used to generate predictions on quadrants imposed on orthorectified rasters captured at 14 and 18 days after emergence. After spatially interpolating the plant densities, the resultant surfaces were highly correlated to manually-determined plant density (R2 = 0.80). Further correlations were observed with tuber number (r = 0.54 at Butter Hill; r = 0.53 at Horse Foxhole), marketable tuber weight per plant (r = −0.57 at Buttery Hill; r = −0.56 at Horse Foxhole) and the normalized difference vegetation index (r = 0.61). These results show that accurate two-dimensional maps of plant density can be constructed from UAV imagery with high correlation to important yield components, despite the loss of accuracy of FRCNN models in partially merged canopies.