bluuebunny/crossref_metadata_2025 huggingface dataset:
La deliberación parlamentaria
Authors: Chueca Ricardo
Published: November 2022

La deliberación es una actividad humana individual, que se transforma y complica al comunicarla a otro u otros. Esto sucede en todo órgano colegiado y, de modo muy singular, en los parlamentos. Es un modo muy singular de deliberación porque no posee una naturaleza voluntaria: se trate de una actividad necesaria para dotar de voluntad al sujeto soberano sin el que la existencia del Estado no es posible. Su naturaleza ha ido cambiando con la evolución de la institución parlamentaria y con los cambios del entorno, especialmente con la consolidación de los partidos políticos y la conversión del parlamento en un parlamento de grupos. Se describe la situación actual y se propone una visión de la deliberación parlamentaria en donde, junto a su permanencia como recurso de respuesta al reto, se destacan los cambios y transformaciones experimentados en las últimas décadas. Se concluye que la deliberación parlamentaria está tan solo acompañando cambios profundos en los procesos de representación política que deberíamos observar minuciosamente.
PERAN RELAWAN PALIATIF DALAM PERAWATAN PALIATIF PASIEN STROKE
Authors: MARWOKO TRIANANG
Published: November 2022

Abstract

Palliative care is a form of service that aims to improve the quality of life of patients and families from life-threatening diseases. With good knowledge, the role of volunteers in providing palliative care services to patients and their families will be better. This study aims to describe the role of palliative volunteers in the palliative care of stroke patients in Batu City. The population in this study are volunteers who are members of the membership. Determination of the number of samples used is purposive sampling which is determined and selected with certain considerations and goals. Methods of data collection through observation, documentation and interviews. The results showed that the role of volunteers in patient self-care, especially in meeting the basic needs of life, could improve the quality of life of patients. Through mentoring provided by volunteers, especially in counseling, it plays a very important role in reducing the level of patient anxiety so that patients no longer think about the cost of their care because the volunteers have sought assistance or sources of treatment funds.
HUBUNGAN DUKUNGAN KELUARGA DAN PERAWAT DENGAN MOTIVASI PASIEN KANKER KOLOREKTAL DALAM MENJALANI KEMOTERAPI
Authors: MUKHLIS AKHMAD
Published: November 2022

ABSTRACT

Chemotherapy can cause physiological and psychological impacts that cause some patients to feel afraid to do chemotherapy, for this reason, family support and nurse support are needed so that colorectal cancer patients remain motivated to undergo chemotherapy. The purpose of this study was to determine the relationship between family support and nurse support on patient motivation to undergo chemotherapy in colorectal cancer patients at Dr. Saiful Anwar Hospital Malang. The design used is a correlation study to study the relationship between the independent variables of family support and nurse support, the dependent variable is the motivation of patients undergoing chemotherapy. Data collection is a cross sectional study using an instrument in the form of a questionnaire. By using the purposive sampling technique, a sample of 40 people was obtained. The research was conducted at Dr. Saiful Anwar Hospital, Malang. The results of the Rank Spearman correlation test show that there is a relationship between family support and motivation in colorectal cancer patients of 0.347 (p=0.028), the relationship is positive/unidirectional with moderate strength, the better the family support, the better the motivation of colorectal cancer patients undergoing chemotherapy. With family support helps cancer patients to increase their confidence that they are accepted by their families, thus providing motivation to do chemotherapy regularly in order to recover. The results of the Rank Spearman correlation test showed that there was a relationship between nurse support and motivation in colorectal cancer patients of 0.490 (p=0.000), the relationship was positive/unidirectional with moderate strength, the better the nurse support, the better the motivation of colorectal cancer patients undergoing chemotherapy. Because with the support of nurses, it will increase the patient's knowledge about chemotherapy and healing, thereby increasing the patient's motivation to undergo chemotherapy.

 

Keywords: Colorectal cancer, chemotherapy, family support, nurse support, motivation
STUDI LITERATURE TENTANG HUBUNGAN STIGMA DIRI DENGAN PENERIMAAN DIRI PADA ODHA
Authors: MALO BILI MELKIANUS , DARAMATASIA WIRA , SOELISTYONINGSIH DWI
Published: November 2022

Pendahuluan: Stigma merupakan fenomena yang sangat kuat yang terjadi di masyarakat dan erat kaitannya dengan nilai-nilai yang dianut oleh berbagai identitas sosial yang terkait. Penerimaan diri merupakan suatu kondisi dimana seseorang memiliki sikap positif terhadap dirinya sendiri, mengakui dan menerima berbagai kekurangan dan kelebihan yang dimilikinya serta mampu berpikir positif tentang kehidupan yang dijalaninya.

Tujuan penelitian: Studi Pustaka Penelitian ini bertujuan untuk mengetahui hubungan antara stigma diri dengan penerimaan diri pada ODHA.

Metode penelitian: Literatur penelitian ini diperoleh dari 3 database yaitu science direct, pubmed dan googleschool. Cari artikel penelitian ilmiah dari tahun 2015-2020 menggunakan kriteria inklusi dan eksklusi. Penelitian sebelumnya menggunakan desain cross sectional, deskriptif kuantitatif, deskriptif kualitatif dan pretest-posttest.

Hasil penelitian: Berdasarkan 10 literatur terkait stigma diri dan penerimaan diri (n = 10), ditemukan bahwa sebagian besar stigma yang dirasakan pada ODHA memiliki tingkat personalisasi stigma, sikap publik dan citra diri negatif yang rendah, sedangkan pengungkapan kekhawatiran memiliki tingkat yang tinggi. Sementara itu. tidak ada hubungan antara pengetahuan HIV dan AIDS, tingkat pendidikan dan terpaan media dengan stigma terhadap ODHA. Bekerja untuk kepentingan keluarga, intervensi di lingkungan pedesaan dan tempat kerja, serta dukungan sosial yang nyata diperlukan untuk meminimalkan stigma diri pada ODHA. Usia dan pendidikan mempengaruhi persepsi ODHA dan hasil positif untuk ODHA dan CFM terbukti bermanfaat karena ODHA merasa lebih didukung dan CFM menunjukkan kasih sayang yang lebih terhadap ODHA. Ada pengaruh antara penerimaan diri pada ODHA dengan kecerdasan emosional. Pengaruh positif dan negatif terhadap stres kerja karyawan hingga 37,8%. Partisipan yang merupakan mantan WPS (wanita pekerja seks) digambarkan dalam tiga tema dengan merasakan tekanan batin saat mengetahui dirinya positif HIV, memotivasi diri dan mendapatkan dukungan dari orang terdekatnya, serta menerima dan membuka statusnya sebagai ODHA. Cara ODHA memproses hasil positif penyakitnya dapat mempengaruhi keterlibatannya dalam pengobatan dan perawatan HIV/AID.

Kesimpulan: Berdasarkan 10 jurnal yang di-riview menunjukkan usia dan Pendidikan mempengaruhi persepsi ODHA dan CFM terbukti bermanfaat untuk menunjukkan lebih banyak kasih saying terhadap ODHA. Ada pengaruh antara penerimaan diri pada ODHA dengan kecerdasan emosional.
Fetal Pericardiocentesis
Authors: Garcia Rodriguez Raquel , Garcia Delgado Raquel , Obreros Zegarra Luciana , Emergui Zhrizen Yonit , Armas Roca Marta , Castellano Medina Margarita , Garcia Hernandez Jose Angel
Published: November 2022

Fetal pericardiocentesis is a safe and effective procedure that is used to drain pericardial effusion in selected fetuses. The aim of the procedure is to reduce the risk of pulmonary hypoplasia, the development of cardiac tamponade and fetal hydrops, and in some cases to allow fetal lung maturity, improving fetal extraction with a better haemodynamic and respiratory condition. In this review, we discuss the indications, technical procedure, and the outcomes of the fetal pericardiocentesis reported in the literature.
Virtual Reality Assisted Anaesthesia During Upper Gastrointestinal Endoscopy: Report of 115 Cases
Authors: Mosso Vázquez José Luis , Wiederhold Brenda K. , Miller Ian , Wiederhold Mark D.
Published: November 2022

Objective: The utility of virtual reality (VR) pain management to reduce visceral or autonomic responses is presented in 115 cases during diagnostic upper gastrointestinal (GI) endoscopy.

Methodology: 115 patients with peptic disease and gastro-oesophageal reflux were given an upper GI endoscopy with local anaesthesia. They were divided into two groups, 56 treated with VR and 59 without VR during procedures. A 10-point Visual Analogue Scale (VAS) for pain was administered to patients and the physician rated level of stress on a 3-point scale.

Results: Overall, visceral responses during oesophageal, stomach, and duodenum endoscopy were reduced using VR. Overall pain was significantly lower in the VR group than the control group with a moderate effect size. Physician stress was also reduced in the VR group, allowing greater accuracy and a shorter procedure time. A total of 115 satisfactory GI endoscopy procedures were carried out with no complications.

Conclusions: VR therapy considerably reduces the need for medication, effectively lowering costs for public health institutions and decreasing patient complications and recovery time.
Drug-Coated Balloons and Coronary Bifurcation Lesions
Authors: Durante Alessandro , Laforgia Pietro Leonida
Published: November 2022

Bifurcation coronary lesions still represent a challenge for interventional cardiologists. Although provisional stenting is considered the gold standard for the treatment of bifurcations, some studies report good results with two-stent techniques. In the last few years, drug-coated balloons have been used for the treatment of several kinds of coronary lesion, such as in-stent restenosis, small vessels, and bifurcations. The use of drug coated balloons for the treatment of the side branch after provisional stenting is a promising option for further improving provisional stenting results.
Dual Antiplatelet Therapy Challenges in Complex Clinical Scenarios
Authors: Saul Helen
Published: November 2022

The symposium was a practical, case-based discussion in which four panellists each presented a real and challenging case regarding dual antiplatelet therapy (DAPT) strategy. The audience and other panel members were invited to give their thoughts on the difficult decisions that arose during treatment.

Case 1, presented by Dr Chevalier, was a man with multivessel disease, enrolled in the Discovery 1TO3 trial.1 He received staged treatment with three stents; strut coverage was evaluated with optical frequency domain imaging (OFDI). Four days after the staged percutaneous coronary intervention (PCI), the patient suffered a Bleeding Academic Research Consortium (BARC) Type 3a gastric bleeding event and the gastroenterologist requested that the DAPT be stopped.

Dr Vranckx presented Case 2, in which a man, aged 84 years, presented 5 hours after myocardial infarction. He had a bifurcation lesion and was treated with a two-stent technique. Bleeding and ischaemic risk scores varied according to the risk scoring system used, but overall this patient had a high bleeding risk (HBR) and reasonable ischaemic risk. Treatment had to balance the two risks.

Case 3, presented by Dr Cuisset, was a 62-year-old male with chronic coronary artery disease (CAD), alcohol abuse, and Type 2 diabetes mellitus with poor adherence to metformin. The patient had good haemoglobin levels and renal function, and moderate liver abnormalities. He had thrombocytopenia and stable angina with prior documentation of ischaemia under stress. There was no significant lesion on the left anterior descending artery (LAD) or left coronary artery, but a 60–70% stenosis of the mid-right coronary artery (RCA). He was likely to be nonadherent to antiplatelet therapy.

Dr Colombo presented the fourth case, in which a 73-year-old male had a large thoracoabdominal aortic aneurysm and had been referred from vascular surgeons. He had standard risk factors for CAD, reasonable ejection fraction (EF), and no significant valvular disease. Preoperative coronary angiography showed a noncritical stenotic lesion on RCA, a 90% stenotic lesion mid-left circumflex artery (LCX), and total occlusion of LAD. PCI ahead of vascular surgery could involve one, two, or three vessels.
Desain Motif sebagai Brand Strategy Pengembangan Galeri Sulam Pinjaik Patah Kabupaten Agam Sumatra Barat
Authors: Masdupi Erni , Kurniawati Tri , Fitra Halkadri , Faisal Dini
Published: November 2022

Abstrak Pinjaik patah atau jarum jahit yang dipatahkan merupakan brand yang cukup dikenal untuk persoalan produk sulaman terawang di Sumatra Barat, khususnya di kabupaten Agam, Nagari Panampuang. Sayangnya, pengembangan motif yang dilakukan masih minim sehingga diversifikasi produk, pembeda produk hasil sulaman pinjaik patah dan hasil produk kompetitor tidak dapat dibedakan. Perlu dirancang sebuah desain motif baru yang berangkat dari picture mark logo galeri sulam pinjaik patah dengan tujuan untuk menciptakan diversifikasi produk dan brand awareness sulaman pinjaik patah. Metode yang digunakan adalah research and development dengan tahapan 1) Potensi dan Masalah; 2) Pengumpulan informasi; 3) Pengembangan model; 4) Validasi model; 5) Uji coba model. Hasil yang didapatkan yaitu perajin sulaman dapat mengaplikasikan desain motif dalam jenis sulaman terawang dan sulaman menggunakan bordir, hanya saja masih diperlukan revisi dan latihan dari perajin sulaman agar dapat menghasilkan motif yang harmonis. Pengembangan desain dan produk sebaiknya difokuskan pada sulaman terawang yang menjadi unggulan sulam pinjaik patah. Kata kunci: brand awareness, desain motif, pinjaik patah, sulaman AbstractPinjaik patah or a broken sewing needle is a well-known brand for embroidery products in West Sumatra, especially in Agam district, Nagari Panampuang. Unfortunately, the development of the motifs is still minimal so that product diversification, the difference between the product of pinjaik patah embroidery and the product of competitors cannot be distinguished. It is necessary to design a new motif design that departs from the picture mark of the Pinjaik Patah embroidery gallery logo with the aim of creating product diversification and brand awareness of Pinjaik Patah embroidery. The method used is research and development which includes five steps like 1) potentials and problems; 2) gathering information; 3) designing; 4) validation and; 5) design testing. The results obtained are that the embroiderer can apply the motif design in the type of overlay embroidery and embroidery using embroidery, it's just that revision and practice from the embroiderer is still needed in order to produce a harmonious motif. Design and product development should be focused on terawang embroidery which is the flagship of pinjaik patah embroidery. Keywords: brand awareness, embroidery, motif design, pinjaik patah
Implementasi Internet of Things Dalam Mengontrol dan Memonitor Ketinggian Air
Authors: Zakaria Alamsyah , Sholeh Abd. Rahman , Ngemba Hajra Rasmita , Setijadi Eko
Published: November 2022

Peningkatan kualitas suatu alat dibidang industri sangat diperlukan dalam menilai keakuratan dan efisiensi suatu produk. Salah satu bidang industri yang membutuhkan sistem pengontrolan dan monitoring adalah tempat penampungan air. Sistem ini sangat diperlukan untuk mengetahui kondisi ketinggian air. Umumnya pengguna dalam melakukan pengisian dan pemantauan air hanya memanfaatkan mesin pompa yang dikendalikan secara manual. Permasalahan tersebut membutuhkan waktu yang lama, terjadi pemborosan daya listrik, air, dan dapat menimbulkan korsleting listrik yang disebabkan oleh air meluap dari penampungan. Untuk meningkatkan keamanan dan efisiensi waktu diperlukan suatu rancangan teknologi yang dapat mengontrol dan memonitor ketinggian air secara realtime. Modul yang digunakan dalam mengontrol batas maksimal ketinggian air menggunakan arduino uno. Sedangkan untuk memonitor kondisi ketinggian air menggunakan modul ESP32. Penelitian ini bertujuan untuk membantu pengguna dalam mengontrol dan memonitor kondisi ketinggian air melalui smartphone. Rancangan ini dapat meminimalisir terjadinya pemborosan air, daya listrik, dan kebakaran. Hasil penelitian menunjukkan bahwa rancangan bekerja dengan optimal dengan tingkat akurasi 98,96%
Pengembangan Website Joy Fellowship Indonesia dengan Metode Uji Usabilitas
Authors: Actaviana Anggita Irent , Delima Rosa , Krisnawati Lucia Dwi
Published: November 2022

Website JOY Indonesia merupakan media untuk mendapatkan informasi terkait kegiatan JOY serta digunakan sebagai media promosi untuk menjangkau anggota baru dan tetap terhubung dengan para-alumni. Salah satu faktor ketertarikan pengguna untuk mengakses website dan menjadi ciri khas adalah antarmuka, namun saat ini website JOY masih jarang diakses dan juga masih terdapat kekurangan pada website terutama pada fungsionalitasnya. Terkait masalah tersebut akan dilakukan pengukuran tingkat usabilitas antarmuka situs web JOY Indonesia dengan metode Usability Testing, kemudian akan dilakukan perancangan purwarupa baru berdasarkan hasil uji usabilitas tersebut. Penelitian ini akan dilakukan dengan menguji empat aspek usabilitas yaitu time on task, task success, error dan efficiency, serta dilakukan wawancara dan observasi terhadap responden untuk mengetahui tingkat kebergunaan situs web JOY Indonesia. Pengujian akhir yang dilakukan pada desain pengembangan memiliki hasil efisiensi sebesar 88.34%, kemudian hasil pengujian time on task pada desain hasil pengembangan total waktu keseluruhannya adalah sebesar 139,8 detik, dan nilai success rate sebesar 94.80%. Hasil tersebut sudah memenuhi rata-rata tingkat keberhasilan serta fungsionalitasnya dapat diakses dengan efisien dan efektif oleh pengguna.
Identifikasi Pohon Tropis di Daerah Perkotaan Menggunakan Multispectral Drone Imagery
Authors: Abidin Zainal , Fatchurrohman Fatchurrohman , Aziz Okta Qomaruddin
Published: November 2022

Vegetasi di daerah perkotaan tumbuh diantara gedung dan jalan. Bangunan di sekitar pohon berdampak pada identifikasi tanaman menggunakan citra drone. Paper ini menjelaskan tentang perbandingan kemampuan citra drone pada band cahaya tampak, near infrared, dan red-edge untuk identifikasi pohon tropis di daerah perkotaan. Kami menyusun dataset yang berisi paduan pohon saman (Samanea Saman), cemara (Casuarina equisetifolia), dan pohon-lain. Setiap citra melalui tahapan proses filtering, segmentasi, dan classification. Hasil uji coba menunjukkan bahwa band cahaya tampak dapat mengidentifikasi saman, cemara, dan pohon-lain dibandingkan dengan citra pada band near-infrared dan red-edge.
Penerapan Algoritma C4.5 Untuk Memprediksi Keluhan Pelanggan Pada Apartemen Jakarta
Authors: Sunarti Sunarti , Handayanna Frisma
Published: November 2022

Ketidakpuasan pelanggan merupakan masalah yang dihadapi oleh pengelola Apartemen. Ketidakpuasan dapat menimbulkan keluhan dan merugikan dalam bisnis. Penelitian menggunakan metode algoritma C4.5. Metode ini digunakan menentukan pengambilan keputusan untuk memprediksi keluhan pelanggan, diketahui tingkat akurasi dan presisinya. Data dianalisis menggunakan aplikasi Rapidminer. Rapidminer adalah perangkat lunak dengn open source. Tujuan dari penelitian ini ialah untuk mengatasi keluhan pelanggan apartemen atas ketidakpuasan terhadap pelayanan yang diberikan. Atributnya adalah nounit, kewarganegaraan, status pernikahan, pekerjaan, alamat pekerjaan, jumlah penghuni, dan jenis kelamin dengan keputusan komplain dan tidak komplain. Peroleham pengujian model yang terbentuk akurat hingga 88,13 dengan kumpulan hasil pengolahan ROC (Receiver Operating Characteristic) menggunakan data training dengan besarnya 0,953 tingkat diagnostik klasifikasi sangat baik. Model pohon keputusan yang ada bisa meningkatkan akurasi untuk memprediksi keluhan pelanggan pada Apartemen Jakarta.
Dancer vs Non Dancer: Korelasi Kontroler Terhadap Game Experience Pada Rhythm Game
Authors: Ni'maturahmah Musyaffa , Rizky .
Published: November 2022

Dalam penelitian ini, penulis akan meneliti tentang korelasi kontroler terhadap game experience pada rhythm game dimana untuk mengetahui lebih efektif penggunaan kontroler kamera ipad atau nintendo switch. Dengan menggunakan metode CEGE atau kuisioner yang terbagi atas beberapa bagian yaitu enjoyment, frustration, Puppetry, dan bagian Video-Game yang diberikan terhadap 22 responden dengan 38 pertanyaan. Hasil dari penelitian didapat bahwa untuk pemain pemula atau non-dancer akan lebih nyaman menggunakan kamera ipad, sedangkan pemain dancer atau yang ahli lebih nyaman menggunakan joycon. Dengan adanya penelitian ini diharapkan dapat menjadi acuan bagi penelitian yang serupa.
The Role of the School Environment in the Formation of Student Character Values at SDN Gelaran 2
Authors: Sagita Riski Rismala , Rochmania Desty Dwi
Published: November 2022

Character education in the current era is very much needed, because the Indonesian nation is experiencing a character crisis in the nation's children which results in the destruction of the Indonesian nation itself. In this case the school environment can be used as a means of building student character values. This research uses a case study qualitative approach. Data were collected using observation, interviews, and documentation methods. The purpose of this study is to describe the process and results of the formation of student character values ​​through the school environment at SDN Gelaran 2. The results of this study are (1) The formation of character values ​​for SDN Gelaran 2 students through the school environment is realized through habituation activities, examples, regulations made by teachers and schools, extracurricular activities, and also through the learning process in the classroom. The supporting and inhibiting factors in the process of forming student character values ​​through the school environment are the family environment, the community environment, and the children themselves (2) The results of the formation of student character values ​​through the school environment are very good. With the formation of student character values ​​through this school environment, students can behave well, be more polite and courteous to teachers and friends, students respect and respect each other more, students have a 5S culture (smile, greeting, greeting, polite, and courteous), and obey the rules. The polite behavior of students is shown by student attitudes such as greeting the teacher when passing by, respecting the teacher by walking behind him, saying excuse me when walking in front of the teacher, knocking on the door before entering the classroom, taking something with his right hand, looking down when the teacher is giving advice.
The Development Of Water Cycle Diorama Media To Improve Student Learning Outcomes
Authors: Islamiyah Latifatul , Asmarani Ratih
Published: November 2022

The development of diorama media asan effort to provide education on water cycle diorama media is based on several gaps including students who have never received interesting education on water cyclematerial, diorama media are not available, on average students do not understand the water cycle process. Development of diorama media and implementation for students of SDN Sukorejo1Perak.the development aims to producevalid diorama media. thedevelopment of this diorama media uses the Borg and Gall development model. There are 8 development processes that have been implemented in this research, namely, 1) potential and problems, 2) gathering information, 3) product design, 4) design validation , 5) design improvement, 6) product testing, 7) product revision, 8)trial use. The assessment of the diorama media was carried out by instrument experts, material experts, media experts, users of educators, as well as fifth grade students of SDN Sukorejo 1 Perak. The aresults obtained by the media expert validator is 95,58%, the material expert validator is 82,35 %. The results of the percentage of instrument expert validators are 80%, the results of user of educator trials are 77,7%. The results of small group trials were conducted with 8 studens witha percentage of91,5%, field trials were conducted with 16 students with a percentage of 90%. Based on the results of theoverall data analysis, it can be concluded that the development of diorama media is feasible to beused as a learning medium.
DEVELOPMENT OF EDUCATIONAL GAMES BASED ON INTERACTIVE POWERPOINT MATERIALS OF SUBSTANCES AND ITS CHANGES
Authors: Rohmatul Lailiyah  , Nur Hayati
Published: November 2022

Learning media aims as a communication tool to convey a material in learning. Developing an Interactive PowerPoint-based Educational Game Media is one of the general objectives of this research. The development model used in this research is ADDIE (Analysis, Design, Development, Implementation, and Evaluation). However, the Implementation phase was not carried out due to the pandemic. The data obtained are qualitative data types (from the media development process) and quantitative data (from data validation results by media experts). This research resulted in a product in the form of an interactive PowerPoint-based educational game media material substance, substance and its changes, which have been validated by several experts, namely material expert lecturers, media expert lecturers and science teachers at Al-Furqan Madrasatul Qur'an Tebuireng Jombang Middle School. With a validation score of 2.98 out of 4.00 which is categorized as valid.
Nucleobase-modified nucleosides and nucleotides: Applications in biochemistry, synthetic biology, and drug discovery
Authors: Berdis Anthony
Published: November 2022

Abstract. DNA is often referred to as the “molecule of life” since it contains the genetic blueprint for all forms of life on this planet. The core building blocks composing DNA are deoxynucleotides. While the deoxyribose sugar and phosphate group are ubiquitous, it is the composition and spatial arrangement of the four natural nucleobases, adenine (A), cytosine (C), guanine (G), and thymine (T), that provide diversity in the coding information present in DNA. The ability of DNA to function as the genetic blueprint has historically been attributed to the formation of proper hydrogen bonding interactions made between complementary nucleobases. However, recent chemical and biochemical studies using nucleobase-modified nucleotides that contain “non-hydrogen bonding” functional groups have challenged many of the dogmatic views for the necessity of hydrogen-bonding interactions for DNA stability and function. Based on years of exciting research, this area has expanded tremendously and is thus too expansive to provide a comprehensive review on the topic. As such, this review article provides an opinion highlighting how nucleobase-modified nucleotides are being applied in diverse biomedical fields, focusing on three exciting areas of research. The first section addresses how these analogs are used as mechanistic probes for DNA polymerase activity and fidelity during replication. This section outlines the synthetic logic and medicinal chemistry approaches used to replace hydrogen-bonding functional groups to examine the contributions of shape/size, nucleobase hydrophobicity, and pi-electron interactions. The second section extends these mechanistic studies to provide insight into how nucleobase-modified nucleosides are used in synthetic biology. One example is through expansion of the genetic code in which changing the composition of DNA makes it possible to site-specifically incorporate unnatural amino acids bearing unique functional groups into enzymes and receptors. The final section describes results of pre-clinical studies using nucleobase-modified nucleosides as potential therapeutic agents against diseases such as cancer.
Early prevention of radial artery occlusion via distal transradial access for primary percutaneous coronary intervention
Authors: Wang Yujie , Liu Zijing , Wu Yongxia , Li Zixuan , Wang Yuntao , Wang Senhu , Xu Rong , Zhang Libin , Wang Yuping , Guo Jincheng
Published: November 2022

Background

Conventional transradial access (TRA) has been the preferred access for coronary intervention. Recently, distal radial access (DRA) is introduced as an alternative choice to reduce radial artery occlusion (RAO) risk. The study sought to assess the impact of DRA on early RAO using Doppler ultrasound in patients with ST-segment elevation myocardial infarction (STEMI) who underwent primary percutaneous coronary intervention (PCI).

Methods

This is a prospective, single-center, open-label randomized clinical trial in which patients with indications for primary PCI from January 2022 to September 2022 were assigned to DRA or TRA group with 100 cases in each group. The primary endpoint was the incidence of forearm RAO, evaluated by Doppler ultrasound before discharge.

Results

The rate of access success was comparable between the DRA and TRA groups (98.0 vs. 94.0%,P= 0.279). Compared with the TRA group, longer puncture time was observed in the DRA group [2.4 (1.7–4.2) min vs. 1.7 (1.4–2.3) min;P< 0.001] whereas the door-to-wire time was not delayed in primary PCI [71 (54–88) min vs. 64 (56–82) min,P= 0.103]. Shorter hemostasis time was required in the DRA group [3.1 (2.7–3.3) h vs. 6.2 (5.9–6.4) h;P< 0.001]. Significant reduction of the incidence of forearm RAO was observed in the DRA group (2.0 vs. 9.0%,P= 0.030). Local hematomas ≤ 5 cm was similar in both groups (4.0 vs. 6.0%,P= 0.516), while those > 5 cm were significantly more frequent in the TRA group (0 vs. 6.0%,P= 0.029).

Conclusion

Distal radial access is associated with a comparable lower incidence of forearm RAO, shorter hemostasis time, and lower rate of vascular complications compared to TRA in primary PCI.

Systematic review registration

[https://www.chictr.org.cn], identifier [ChiCTR2200061841].
Using Facebook advertising data to describe the socio-economic situation of Syrian refugees in Lebanon
Authors: Fatehkia Masoomali , del Villar Zinnya , Koebe Till , Letouzé Emmanuel , Lozano Andres , Al Feel Roaa , Mrad Fouad , Weber Ingmar
Published: November 2022

While the fighting in the Syrian civil war has mostly stopped, an estimated 5.6 million Syrians remain living in neighboring countries1. Of these, an estimated 1.5 million are sheltering in Lebanon. Ongoing efforts by organizations such as UNHCR to support the refugee population are often ineffective in reaching those most in need. According to UNHCR's 2019 Vulnerability Assessment of Syrian Refugees Report (VASyR), only 44% of the Syrian refugee families eligible for multipurpose cash assistance were provided with help, as the others were not captured in the data. In this project, we are investigating the use of non-traditional data, derived from Facebook advertising data, for population level vulnerability assessment. In a nutshell, Facebook provides advertisers with an estimate of how many of its users match certain targeting criteria, e.g., how many Facebook users currently living in Beirut are “living abroad,” aged 18–34, speak Arabic, and primarily use an iOS device. We evaluate the use of such audience estimates to describe the spatial variation in the socioeconomic situation of Syrian refugees across Lebanon. Using data from VASyR as ground truth, we find that iOS device usage explains 90% of the out-of-sample variance in poverty across the Lebanese governorates. However, evaluating predictions at a smaller spatial resolution also indicate limits related to sparsity, as Facebook, for privacy reasons, does not provide audience estimates for fewer than 1,000 users. Furthermore, comparing the population distribution by age and gender of Facebook users with that of the Syrian refugees from VASyR suggests an under-representation of Syrian women on the social media platform. This work adds to growing body of literature demonstrating the value of anonymous and aggregate Facebook advertising data for analysing large-scale humanitarian crises and migration events.
Construction and validation of a predictive model for hypocalcemia after parathyroidectomy in patients with secondary hyperparathyroidism
Authors: Cheng Jingning , Lv Yong , Zhang Ling , Liu Yafeng
Published: November 2022

Objective

We aimed to construct and validate a predictive model for the risk of hypocalcemia following parathyroidectomy (PTX) for the treatment of secondary(renal) hyperparathyroidism (SHPT).

Methods

Information regarding patients with SHPT who underwent PTX between January 2019 and April 2022 was collected retrospectively. Univariate and multivariate logistic regression analyses were used to identify independent risk factors for hypocalcemia following PTX and to construct predictive models. The areas under the receiver operating characteristic curve (AUC), the calibration curve, and the clinical decision curve (decision curve analysis, DCA) were used to assess the discrimination, calibration, and level of clinical benefit obtained using the predictive models.

Results

We studied 238 patients who were randomly allocated in a 7:3 ratio to a training group (n=166) and a test group (n=72). Univariate and multivariate logistic regression analyses were performed, in which three variables (the circulating parathyroid hormone (PTH) and Ca concentrations, and alkaline phosphatase (ALP) activity) were interrogated for possible roles as independent risk factors for hypocalcemia in patients with SHPT who undergo PTX, and used to construct predictive models. The AUCs for the constructed models were high for both the training (0.903) and test (0.948) groups. The calibration curve showed good agreement between the incidence of postoperative hypocalcemia estimated using the predictive model and the actual incidence. The DCA curve indicated that the predictive model performed well.

Conclusion

A predictive model constructed using a combination of preoperative PTH, Ca, and ALP may represent a useful means of identifying patients with SHPT at high risk of developing hypocalcemia following PTX in clinical practice.
Cardiovascular disease risk in offspring of polycystic ovary syndrome
Authors: Shawky Noha M.
Published: November 2022

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder affecting women at reproductive age. PCOS diagnosis (Rotterdam criteria) is based on the presence of two out of three criteria; clinical and/or biochemical hyperandrogenism, oligo- or an-ovulation and polycystic ovaries. PCOS women suffer from a constellation of reproductive and metabolic abnormalities including obesity and insulin resistance. PCOS women also have increased blood pressure and increased risk of cardiovascular diseases (CVD). In-utero, offspring of PCOS women are exposed to altered maternal hormonal environment and maternal obesity (for most of PCOS women). Offspring of PCOS women could also be subject to genetic susceptibility, the transgenerational transmission of some of the PCOS traits or epigenetic changes. Offspring of PCOS women are commonly reported to have an abnormal birth weight, which is also a risk factor for developing CVD and hypertension later in life. Although studies have focused on the growth pattern, reproductive and metabolic health of children of PCOS women, very limited number of studies have addressed the risk of hypertension and CVD in those offspring particularly as they age. The current narrative review is designed to summarize the available literature (both human studies and experimental animal studies) and highlight the gaps in addressing hypertension and CVD risks in offspring of PCOS women or hyperandrogenemic female animal models.
Vertical green system for gray water treatment: Analysis of the VertiKKA-module in a field test
Authors: Aicher Andreas , Boermel Melanie , Londong Jörg , Beier Silvio
Published: November 2022

This work presents a modular Vertical Green System (VGS) for gray water treatment, developed at the Bauhaus-Universität Weimar. The concept was transformed into a field study with four modules built and tested with synthetic gray water. Each module set contains a small and larger module with the same treatment substrate and was fed hourly. A combination of lightweight structural material and biochar of agricultural residues and wood chips was used as the treatment substrate. In this article, we present the first 18 weeks of operation. Regarding the treatment efficiency, the parameters chemical oxygen demand (COD), total phosphorous (TP), ortho-phosphate (ortho-P), total bound nitrogen (TNb), ammonium nitrogen (NH4-N), and nitrate nitrogen (NO3-N) were analyzed and are presented in this work. The results of the modules with agricultural residues are promising. Up to 92% COD reduction is stated in the data. The phosphate and nitrogen fractions are reduced significantly in these modules. By contrast, the modules with wood chips reduce only 67% of the incoming COD and respectively less regarding phosphates and the nitrogen fraction.
Using loop-primer mediated PCR to enhance the detection of poorly preserved DNA
Authors: Xiang Hai , Wang Zhi , Yang Liu , Zhang Xing , Zhao Xingbo
Published: November 2022

Ancient DNA is vitally important in evolutionary research, and obtaining authentic ancient DNA sequences is critical for a proper analysis. However, it is difficult to acquire amplicons accurately and efficiently from ancient DNA templates using current techniques. Here, we established a loop-primer-mediated amplification method (L-PCR) to obtain target ancient DNA sequences with high accuracy and efficiency. The method was tested using 66 ancient samples (including 27 pig bones or teeth and 39 chicken bones) and serially diluted modern animal DNA templates. Compared to nested PCR, L-PCR was proven to be more efficient and accurate and could obtain more amplicons from both ancient pig samples and chicken bones and detect as low as 10−3 ng/μl modern pig template DNA. The efficiency was at least 100-fold that of the nested PCR. The results suggest that L-PCR is advantageous for obtaining authentic DNA sequences from poorly preserved or recalcitrant ancient specimens.
Identification of human placenta-derived circular RNAs and autophagy related circRNA-miRNA-mRNA regulatory network in gestational diabetes mellitus
Authors: Bao Yindi , Zhang Jun , Liu Yi , Wu Lianzhi , Yang Jing
Published: November 2022

Gestational diabetes mellitus (GDM) is a metabolic and reproductive disease with serious risks and adverse health effects. However, the pathophysiological mechanism of GDM, especially the roles of circRNAs in its pathogenesis, is largely unknown. The objective of this study was to identify and investigate the roles of circRNAs in GDM. In the current study, placental circRNA expression profiles of normal controls and GDM patients were analyzed using high-throughput sequencing. Bioinformatics analysis identified a total of 4,955 circRNAs, of which 37 circRNAs were significantly deregulated in GDM placentas compared with NC placentas. GO and KEGG enrichment analyses demonstrated that metabolic process-associated terms and metabolic pathways that may be related to GDM were significantly enriched. The biological characteristics of placenta-derived circRNAs, such as their stability and RNase R resistance, were also validated Bioinformatics prediction. Moreover, we constructed the autophagy related circRNA-miRNA-mRNA regulatory network and further functional analysis revealed that the circCDH2–miR-33b-3p–ULK1 axis may be associated with autophagy in the placentas of GDM patients. Our study indicates that aberrant expression of circRNAs may play roles in autophagy in GDM placentas, providing new insights into GDM.
Cutting through the weeds: Evaluation of a novel adsorption with crossmatch cells and elution protocol to sharpen HLA antibody identification by the single antigen bead assay
Authors: Liwski Robert S. , Tafulo Sandra , Carroll Robert , Lan James H. , Greenshields Anna L.
Published: November 2022

The single antigen bead (SAB) assay is the most used test for the identification of HLA specific antibodies pre- and post-transplant. Nevertheless, detection of spurious reactivities remains a recognized assay limitation. In addition, the presence of weak reactivity patterns can complicate unacceptable antigen assignment. This work presents the evaluation of the adsorption with crossmatch cells and elution (AXE) technique, which was designed to help differentiate weak HLA specific antibodies targeting native antigens from spurious and background SAB assay reactivity. The AXE protocol uses selected donor cells to adsorb HLA specific antibodies from sera of interest. Bound antibodies are then eluted off washed cells and identified using the SAB assay. Only antibodies targeting native HLA are adsorbed. Assay evaluation was performed using five cell donors and pooled positive control serum. AXE efficiency was determined by comparing SAB reactivity of adsorbed/eluted antibody to that of the antibodies in unadsorbed sera. A robust efficiency was seen across a wide range of original MFI for donor specific antibodies (DSA). A higher absorption/elution recovery was observed for HLA class I antigens vs. class II. Locus-specific variation was also observed, with high-expression HLA loci (HLA-A/B/DR) providing the best recovery. Importantly, negligible reactivity was detected in the last wash control, confirming that AXE eluates were not contaminated with HLA antibody carry-over. Donor cells incubated with autologous and DSA-containing allogeneic sera showed that AXE selectively adsorbed HLA antibodies in a donor antigen-specific manner. Importantly, antibodies targeting denatured epitopes or other non-HLA antigens were not detected by AXE. AXE was particularly effective at distinguishing weak HLA antibodies from background reactivity. When combined with epitope analysis, AXE enhanced precise identification of antibody-targeted eplets and even facilitated the characterization of a potential novel eplet. Comparison of AXE to flow cytometric crossmatching further revealed that AXE was a more sensitive technique in the detection of weak DSA. Spurious reactivities on the current SAB assay have a deleterious impact on the assignment of clinically relevant HLA specificities. The AXE protocol is a novel test that enables users to interrogate reactive patterns of interest and discriminate HLA specific antibodies from spurious reactivity.
Targeted screening of genetic associations with COVID-19 susceptibility and severity
Authors: Li Ping , Ke Yuehua , Shen Wenlong , Shi Shu , Wang Yahao , Lin Kailin , Guo Xinjie , Wang Changjun , Zhang Yan , Zhao Zhihu
Published: November 2022

The COVID-19 pandemic has resulted in great morbidity and mortality worldwide and human genetic factors have been implicated in the susceptibility and severity of COVID-19. However, few replicate researches have been performed, and studies on associated genes mainly focused on genic regions while regulatory regions were a lack of in-depth dissection. Here, based on previously reported associated variants and genes, we designed a capture panel covering 1,238 candidate variants and 25 regulatory regions of 19 candidate genes and targeted-sequenced 96 mild and 145 severe COVID-19 patients. Genetic association analysis was conducted between mild and severe COVID-19 patients, between all COVID-19 patients and general population, or between severe COVID-19 patients and general population. A total of 49 variants were confirmed to be associated with susceptibility or severity of COVID-19 (p < 0.05), corresponding to 18 independent loci. Specifically, rs1799964 in the promoter of inflammation-related gene TNF, rs9975538 in the intron of interferon receptor gene IFNAR2, rs429358 in the exon of APOE, rs1886814 in the intron of FOXP4-AS1 and a list of variants in the widely reported 3p21.31 and ABO gene were confirmed. It is worth noting that, for the confirmed variants, the phenotypes of the cases and controls were highly consistent between our study and previous reports, and the confirmed variants identified between mild and severe patients were quite different from those identified between patients and general population, suggesting the genetic basis of susceptibility and severity of SARS-CoV-2 infection might be quite different. Moreover, we newly identified 67 significant associated variants in the 12 regulatory regions of 11 candidate genes (p < 0.05). Further annotation by RegulomeDB database and GTEx eQTL data filtered out two variants (rs11246060 and rs28655829) in the enhancer of broad-spectrum antiviral gene IFITM3 that might affect disease severity by regulating the gene expression. Collectively, we confirmed a list of previously reported variants and identified novel regulatory variants associated with susceptibility and severity of COVID-19, which might provide biological and clinical insights into COVID-19 pathogenesis and treatment.
No causal association between tea consumption and 7 cardiovascular disorders: A two-sample Mendelian randomization study
Authors: Cai Dongsheng , Chen Jun , Wu Yuteng , Jiang Chenyang
Published: November 2022

Background: Previous studies have reported inconsistent results on the causal association between habitual tea consumption and the risk of cardiovascular disease (CVD). This study is aim to determine the association between habitual tea intake and CVD using two-sample Mendelian randomization (MR) analysis.

Methods: The genetically predicted causation between tea consumption and 7 common cardiovascular diseases (atrial fibrillation, hypertension, acute myocardial infarction, coronary atherosclerosis, peripheral vascular disease, angina, and heart failure) was evaluated using MR analysis model. We performed a total of 9 MR analysis methods to analyze the final results. The IVW methods was used as the primary outcome. The other MR analysis method (simple mode, weighted mode, simple median, weighted median, penalized weighted median, MR Egger, and MR-Egger (bootstrap)) were performed as the complement to IVW. Also, the robustness of the MR analysis results was assessed using a leave-one-out analysis.

Results: The IVW analysis methods indicated that there is no causal association between tea consumption and risk of CVD (AF: OR, 0.997, 95% CI, 0.992–1.0001, p = 0.142; hypertension: OR, 0.976, 95% CI, 0.937–1.017, p = 0.242; AMI: OR, 0.996, 95% CI, 0.991–1.000, p = 0.077; CA: OR, 1.001, 95% CI, 0.993–1.009, p = 0.854; PVD: OR, 1.002, 95% CI, 1.000–1.005, p = 0.096; angina: OR, 0.999, 95% CI, 0.993–1.006, p = 0.818; HF: OR, 0.999, 95% CI, 0.996–1.002, p = 0.338). The other MR analysis method and further leave-one-out sensitivity analysis suggested the results were robust.

Conclusion: This MR study indicated that there was no genetically predicted causal association between habitual tea intake and risk of CVD.