bluuebunny/crossref_metadata_2025 huggingface dataset:
The Assessment of Food Addiction and the Yale Food Addiction Scale in Bariatric Surgery Populations
Authors: Stanley Paul , O'Donovan Analise , Schwartz Jeffrey , Edwards-Hampton Shenelle
Published: November 2021

Food addiction (FA) appears among bariatric weight loss surgery candidates who struggle to control the intake of hyperpalatable/refined foods have high rates of psychopathology and related health problems. Despite this, prevalence rates of FA in the bariatric sector are reported as low or variable. We investigated the prevalence of FA and the applicability of conventionally used metrics for 166 pre-surgery candidates from a weight management centre (USA) and a major metropolitan hospital (Australia). Self-report measures assessed FA (Yale Food Addiction Scale (YFAS)), body mass index (BMI), disordered eating, addictive personality, psychopathology, and diet. Consistent with prior research, standard YFAS scoring, requiring the endorsement of a distress/impairment (D/I) criterion (FA + D/I), yielded a FA prevalence rate of 12.7%, compared to 37.3% when D/I was omitted (FA − D/I). We compared profiles for those with FA using each scoring method against those ‘without’, who did not meet a minimum of three YFAS symptoms (non-FA ≤ 2). Both methods differentiated those with and without FA on addictive traits, disordered eating and hyperpalatable food consumption. Only FA + D/I differentiated markers of psychological distress or impairment, including depression, anxiety and quality of life. Results indicate a need for further FA research in bariatric settings.
The ‘Muting’ of the Stability and Growth Pact
Authors: ESTELLA Antonio
Published: November 2021

This article proposes the “muting” of the SGP, the framework of rules that the EU has implemented since the coming into being of the European Monetary Union in the fiscal domain. It is argued herein that the system is far from being credible, from the perspective of the law-as-credibility paradigm. Therefore, the legal condition of the SGP should be “muted”. Three proposals to legally mute the SGP are examined in this article. The Open Method of Coordination is used as a useful model that could be followed from now on in the EU fiscal field. The gains in terms of legal credibility would argue in favour of the muting of the SGP and its correlative conversion into an OMC-like system.
Gender-Affirmation and Loving Attention
Authors: Hernandez E. M.
Published: November 2021

In this article I examine the moral dimensions of gender-affirmation. I argue that the moral value of gender-affirmation is rooted in what Iris Murdoch called loving attention. Loving attention is central to the moral value of gender-affirmation because such affirmation is otherwise too fragile or insincere to have such value. Moral reasons to engage in acts that gender-affirm derive from the commitment to give and express loving attention to trans people as a way of challenging their marginalization. In the latter part of the article, I will discuss how my arguments bear on recent arguments by Robin Dembroff and Daniel Wodak on the use of gender-neutral language (Dembroff and Wodak 2018). They argue that we have a duty not to use gender-specific pronouns for anyone. Their conclusion turns, in part, on a rejection of gender-affirmation as a moral duty. The value of gender-affirmation, rooted in our moral perception of trans people, should make us skeptical of this conclusion, in favor of a more nuanced and pluralistic approach to the ethics of gendering.
Is Testimonial Injustice Epistemic? Let Me Count the Ways
Authors: Almagro Holgado Manuel , Navarro Laespada Llanos , de Pinedo García Manuel
Published: November 2021

Miranda Fricker distinguishes two senses in which testimonial injustice is epistemic. In theprimarysense, it is epistemic because it harms the victim as a giver of knowledge. In thesecondarysense, it is epistemic, more narrowly, because it harms the victim as a possessor of knowledge. Her characterization of testimonial injustice has raised the following objection: testimonial injustice is not always anepistemicinjustice, in the narrow, secondary sense, as it does not always entail that the victim is harmed as a knowledge-possessor. By adopting a perspective based on Robert Brandom's normative expressivism, we respond to this objection by arguing that there is a close connection, conceptual and constitutive rather than merely causal, between the primary and the secondary epistemic harms of testimonial injustice, such that testimonial injustice always involves both kinds of epistemic harm. We do so by exploring the logic and functioning of belief and knowledge ascriptions in order to highlight three ways in which the secondary epistemic harm caused by testimonial injustice crystallizes: it undermines the epistemic agency of the victim, the epistemic friction necessary for knowledge, and the possibility of occupying particular epistemic nodes.
About the influences of compressibility, heat transfer and pressure gradients in compressible turbulent boundary layers
Authors: Wenzel Christoph , Gibis Tobias , Kloker Markus
Published: November 2021

This paper presents a comprehensive analysis of the momentum and energy transfer in compressible turbulent boundary layers based on integral identities. By considering data obtained from direct numerical simulations for a wide parameter range, the superordinate influences of compressibility, wall heat transfer and pressure gradient on the terms of the governing equations are identified and visualized. This allows us both to determine to what degree cases corresponding to different Mach number, heat transfer and pressure-gradient conditions have physically comparable behaviour and to design turbulent boundary-layer cases with specific sought-after behaviour. To this end, newly formulated identities for the skin-friction coefficient

$c_f$

and the specific heat-transfer coefficient

$c_h$

from wall-normal integrals based on the non-dimensional compressible momentum and total-enthalpy equations are derived and evaluated. As the individual terms of the resulting identities stay formally close to the terms of the governing equations, the integral analysis further allows the evaluation of common arguments derived from the ‘strong’ Reynolds analogy from an integral perspective. A particular formulation of the Eckert number

$Ec$

is proposed as a similarity parameter, mapping cases with different Mach numbers and wall heat transfer conditions.
On the merging and splitting processes in the lobe-and-cleft structure at a gravity current head
Authors: Dai Albert , Huang Yu-Lin
Published: November 2021

High-resolution simulations are performed for gravity currents propagating on a no-slip boundary to study the merging and splitting processes in the lobe-and-cleft structure at a gravity current head. The simulations reproduce the morphological features observed in the laboratory and provide more detailed flow information to elucidate the merging and splitting processes. Our mean lobe width$\tilde {b}$and mean maximum lobe width$\tilde {b}_{max}$satisfy the empirical relationships$\tilde {b}/\tilde {d}=7.4 Re^{-0.39}_f$and$\tilde {b}_{max}/\tilde {d}=12.6 Re^{-0.38}_f$, respectively, over the front Reynolds number in the range$383 \le Re_f \le 3267$, where the front Reynolds number is defined as$Re_f= \tilde {u}_f \tilde {d} / \tilde {\nu }, \tilde {u}_f$is the front velocity,$\tilde {d}$is the height of the gravity current head and$\tilde {\nu }$is the fluid kinematic viscosity. When measured in terms of the viscous length scale$\tilde {\delta }_\nu = \tilde {\nu }/\tilde {u}^*$, where$\tilde {u}^*$is the shear velocity at the gravity current head, the mean lobe width and the mean maximum lobe width increase with increasing front Reynolds number and asymptotically approach$126 \tilde {\delta }_\nu$and$230 \tilde {\delta }_\nu$at$Re_f=3267$, respectively. The vortical structure inside a lobe has an elongated tooth-like shape and a pair of counter-rotating streamwise vortices are positioned on the left- and right-hand sides of each cleft. For the merging process, it requires the interaction of three tooth-like vortices and the middle tooth-like vortex breaks up and reconnects with the two neighbouring tooth-like vortices. Therefore, a cleft may continually merge with another neighbouring cleft but may never disappear. For the splitting process, even before the new cleft appears, a new born streamwise vortex is created by the parent vortex of opposite orientation and the parent vortex can be either the left part or the right part of the existing tooth-like vortex inside the splitting lobe. The new born streamwise vortex then induces the other counter-rotating streamwise vortex as the new cleft develops. The initiation of the splitting process can be attributed to the Brooke–Hanratty mechanism reinforced by the baroclinic production of vorticity. Depending on the orientation of the parent vortex, the resulting new cleft after the splitting process can shift laterally in the positive or negative spanwise direction along the leading edge of the gravity currents as the lobe-and-cleft structure moves forward in the streamwise direction. For gravity currents propagating on a no-slip boundary, the lobe-and-cleft structure is self-sustaining and the manifestations of the merging and splitting processes are in accord with reported laboratory observations.
Grylloblattidan insects from Sperbersbach and Cabarz (Germany), two new early Permian and insect-rich localities
Authors: Cui Yingying , Brauner Stephan , Schneider Joerg W. , Béthoux Olivier
Published: November 2021

New fossil insect specimens from two new localities in Germany, namely Sperbersbach and Cabarz (Goldlauter Formation; early Permian), belonging to the Grylloblattida, are described. Abundant material is assigned to Pictoborella clara n. gen. n. sp., regarded as closely related to Pictoborella germanica (Prokop et al., 2012) n. comb., from the Saar-Nahe Basin (Germany; early Permian). Liomopterum fuscatum n. sp., represented by fewer specimens, is delimited based on previously published and new data on various Liomopterum spp. Two other Liomopteridae, Uralioma thuringiensis n. sp. and Liomopterites sperbersbachensis n. sp., each known from a single forewing, are also described. Finally, Cabarzopterum magnificus n. gen. n. sp., with unclear familial affinities, is described based on three forewings. The assemblages of grylloblattidan insects at Sperbersbach and Cabarz are generally similar, with differences probably related to different depositional environments and local paleoclimatic conditions.

UUID: http://zoobank.org/25ae125c-6a17-415f-8cf0-4c0507e4f89d
Provenance and depositional history of the Mesozoic Sanjiang Basin (northeastern China): implications for the uplift history of the northeastern Asian continental margin
Authors: Luan Jin-Peng , Rosenbaum Gideon , Fu Jian-Fei
Published: November 2021

The uplift history of northeastern Asian continental margin has been the subject of much debate. The Sanjiang Basin is an ideal area to investigate the uplift history of this margin. We present new data from sandstone petrology, whole-rock geochemistry, U–Pb geochronology and Hf isotopy of detrital zircons to trace the provenance of the basin and to unravel the uplift history of the northeastern Asian continental margin. We investigated the chemical and plagioclase indices of alteration and index of compositional variability (CIA, PIA and ICV, respectively) in samples from two formations. The geochemical proxies indicate that source rocks were subjected to an intensified weathering process. Based on the high SiO2/Al2O3 values and low K2O, we infer that the two basinal strata experienced silicification and insignificant potassium metasomatism. Sandstone petrography is indicative of low degrees of sedimentary sorting, suggesting a proximal deposition. The provenance fingerprints of light rare earth, high-field-strength and transition metal elements indicate that parts of the provenance included recycled sediments, and that first-cycle sediments were derived mainly from felsic rocks with a minor contribution from intermediate and mafic rocks. The combined detrital zircon U–Pb ages and Hf isotopic results constrain the contributions of different source terranes over time. The provenance results, in combination with seismic profile interpretations from the Sanjiang Basin, suggest that the northeastern Asian continental margin experienced at least three stages of uplift, which were driven by subduction initiation of the Paleo-Pacific Ocean during the Jurassic Period, a plate motion change during the Early Cretaceous Epoch, and a shallowing of the slab dip angle during the Late Cretaceous Epoch.
Is there a bilingual disadvantage for word segmentation? A computational modeling approach
Authors: FIBLA Laia , SEBASTIAN-GALLES Nuria , CRISTIA Alejandrina
Published: November 2021

Since there are no systematic pauses delimiting words in speech, the problem of word segmentation is formidable even for monolingual infants. We use computational modeling to assess whether word segmentation is substantially harder in a bilingual than a monolingual setting. Seven algorithms representing different cognitive approaches to segmentation are applied to transcriptions of naturalistic input to young children, carefully processed to generate perfectly matched monolingual and bilingual corpora. We vary the overlap in phonology and lexicon experienced by modeling exposure to languages that are more similar (Catalan and Spanish) or more different (English and Spanish). We find that the greatest variation in performance is due to different segmentation algorithms and the second greatest to language, with bilingualism having effects that are smaller than both algorithm and language effects. Implications of these computational results for experimental and modeling approaches to language acquisition are discussed.
The effects of a sugar-sweetened beverage tax and a nutrient profiling tax based on Nutri-Score on consumer food purchases in a virtual supermarket: a randomised controlled trial
Authors: Eykelenboom Michelle , Olthof Margreet R , van Stralen Maartje M , Djojosoeparto Sanne K , Poelman Maartje P , Kamphuis Carlijn BM , Vellinga Reina E , Waterlander Wilma E , Renders Carry M , Steenhuis Ingrid HM
Published: November 2021

Objective:

To investigate the effects of a sugar-sweetened beverage (SSB) tax and a nutrient profiling tax on consumer food purchases in a virtual supermarket.

Design:

A randomised controlled trial was conducted with a control condition with regular food prices (n 152), an SSB tax condition (n 130) and a nutrient profiling tax condition based on Nutri-Score (n 112). Participants completed a weekly grocery shop for their household. Primary outcome measures were SSB purchases (ordinal variable) and the overall healthiness of the total shopping basket (proportion of total unit food items classified as healthy). The secondary outcome measure was the energy (kcal) content of the total shopping basket. Data were analysed using regression analyses.

Setting:

Three-dimensional virtual supermarket.

Participants:

Dutch adults aged ≥18 years are being responsible for grocery shopping in their household (n 394).

Results:

The SSB tax (OR = 1·62, (95 % CI 1·03, 2·54)) and the nutrient profiling tax (OR = 1·88, (95 %CI 1·17, 3·02)) increased the likelihood of being in a lower-level category of SSB purchases. The overall healthiness of the total shopping basket was higher (+2·7 percent point, (95 % CI 0·1, 5·3)), and the energy content was lower (−3301 kcal, (95 % CI −6425, −177)) for participants in the nutrient profiling tax condition than for those in the control condition. The SSB tax did not affect the overall healthiness and energy content of the total shopping basket (P > 0·05).

Conclusions:

A nutrient profiling tax targeting a wide range of foods and beverages with a low nutritional quality seems to have larger beneficial effects on consumer food purchases than taxation of SSB alone.
The impact of attacks on urban services II: Reverberating effects of damage to water and wastewater systems on infectious disease
Authors: Talhami Michael , Zeitoun Mark
Published: November 2021

This article investigates the effects that attacks during armed conflict which damage water and wastewater services have on the outbreak and transmission of infectious disease. It employs a lens of uncertainty to assess the level of knowledge about the reverberations along this consequential chain and to discuss the relevance to military planning and targeting processes, and to the laws of armed conflict. It draws on data in policy reports and research from a wide variety of contexts, and evidence from protracted armed conflicts in Iraq, Yemen and Gaza. The review finds a strong base of evidence of the impact of attacks on water and wastewater services, and a high level of confidence in information about the transmission of infectious disease. One clear risk identified is when people are exposed to water supplies which are contaminated by untreated wastewater. Obtaining a similar level of confidence about the cause and effect along the full consequential chain is challenged by numerous compounding variables, though there are a number of patterns related to the duration of the armed conflict within which the attacks occur. As the conflict protracts, both the risk of the spread of infectious disease and the evidence base for gauging the reverberating effects becomes stronger, for example. The article concludes that the reverberating effects of damage from an attack can be foreseen in some contexts and can be expected to become more foreseeable over time. The analysis suggests that the most pragmatic path for military institutions and those involved in targeting operations to take this knowledge into account is through a “precautionary approach” which assumes the existence of the reverberating effects, and works them in to the standard information-gathering and planning processes.
New Tool Crafts Fast, Free Flood Maps for the Global South
Authors: Besl J.
Published: November 2021

A new online program can quickly map the outlines of past floods, allowing data-scarce countries to prepare for future disasters.
Frequency Dependent Mantle Viscoelasticity via the Complex Viscosity: Cases From Antarctica
Authors: Lau H. C. P. , Austermann J. , Holtzman B. K. , Havlin C. , Lloyd A. J. , Book C. , Hopper E.
Published: November 2021

Studies of glacial isostatic adjustment (GIA) often use paleoshorelines and present‐day deformation to constrain the viscosity of the mantle and the thickness of the elastic lithosphere. However, several studies focused on similar locations have resulted in different estimates of these physical properties. We argue that these different estimates infer apparent viscosities and apparent lithospheric elastic thicknesses, dependent on the timescale of deformation. We use recently derived relationships between these frequency dependent apparent quantities and the underlying thermodynamic conditions to produce predictions of viscoelastic properties and lithospheric thickness across a broad spectrum of geophysical timescales for two Antarctic locations (Amundsen Sea Embayment and the Antarctic Peninsula). Our predictions are constrained by input from seismic tomography, require the self‐consistent consideration of elastic, viscous, and transient rheological behavior and also include non‐linear steady state viscosity, which have been determined by several laboratories. We demonstrate that when the full spectrum of viscoelasticity is considered, lithospheric thickness displays a significant range across frequency and that transient creep may play an important role across the timescales relevant for the GIA studies we explore. We suggest that observational studies could move toward a framework of determining the frequency dependence of viscoelastic quantities—rather than single, frequency independent values of viscosity. There remains much work to accomplish this both theoretically and observationally, but the eventual result would provide deeper insight into the rheological behavior of Earth.
Vertical Profiles of Ozone Concentrations in the Lower Troposphere Downwind of New York City During LISTOS 2018–2019
Authors: Couillard Maxim H. , Schwab Margaret J. , Schwab James J. , Lu Cheng‐Hsuan (Sarah) , Joseph Everette , Stutsrim Brennan , Shrestha Bhupal , Zhang Jie , Knepp Travis N. , Gronoff Guillaume P.
Published: November 2021

While surface concentrations of ozone are routinely monitored, ozone aloft is infrequently measured, but critical for a full understanding of ozone production and transport. In this study, twenty‐six balloon‐borne ozonesondes were launched near the north shore of central Long Island in the summers of 2018 and 2019 as part of the Long Island Sound Tropospheric Ozone Study (LISTOS). The observed vertical ozone profiles are presented and analyzed with additional data sources and modeling tools, including lidar wind profiles from the New York State Mesonet, back trajectories based on 3 km resolution high‐resolution rapid refresh model data, and surface data, aircraft observations, sonde, and ozone lidar measurements from other LISTOS participants. Special attention is given to the region of interest for ozone pollution in the lower troposphere, from the surface to 2 km altitude. Cases analyzed in detail illustrate events with high ozone levels observed in the lower troposphere, often with pronounced vertical structure in the profile. Specifically, easily discernible layers are identified with ozone excursions of up to 40 ppb over short vertical distances. Analysis indicates that synoptic and local meteorological processes can combine to generate the observed vertical profiles. Hot, sunny days with high‐pressure systems are accompanied by high precursor emissions due to increased power demands, plentiful radiation for photochemistry, and stagnation of synoptic winds. Under these conditions, meso‐ and smaller‐scale flows like low‐level jets and sea/bay/land breeze circulations may dominate synoptic flow to produce shearing and the complex vertical layered structure observed.
Serum exosomes from diabetic kidney disease patients promote pyroptosis and oxidative stress through the miR-4449/HIC1 pathway
Authors: Gao Chan , Wang Benyong , Chen Qi , Wang Ming , Fei Xiao , Zhao Ning
Published: November 2021

Background

Diabetic kidney disease (DKD) is a major contributor to end-stage renal disease. Several microRNAs (miRNAs) have been found to be enriched in exosomes of DKD patients, but it remains unclear if any of these miRNAs play an important role in the pathogenesis of DKD.

Methods

Exosomes from diabetic kidney disease (DKD) patients were isolated, and the expression of miR-4449 was measured by qRT-PCR. Reactive oxygen species (ROS) was determined by DCDFA assay kit, and pyroptosis was measured by quantifying the level of activated caspase 1. mRNA and protein levels were quantified by qRT-PCR and WB.

Results

In this study, we demonstrated that miR-4449 is enriched in the serum exosomes of DKD patients, and these exosomes regulate the expression of pro-inflammatory cytokines, ROS levels, and pyroptosis through miR-4449.

Conclusions

Our study uncovered a novel mechanism for the progression of DKD that is mediated through miR-4449 in serum exosomes, which highlights an important role for exosomes in the pathogenesis of DKD.
Intraoperative neural signals predict rapid antidepressant effects of deep brain stimulation
Authors: Sendi Mohammad S. E. , Waters Allison C. , Tiruvadi Vineet , Riva-Posse Patricio , Crowell Andrea , Isbaine Faical , Gale John T. , Choi Ki Sueng , Gross Robert E. , S. Mayberg Helen , Mahmoudi Babak
Published: November 2021

Deep brain stimulation (DBS) of the subcallosal cingulate (SCC) is a promising intervention for treatment-resistant depression (TRD). Despite the failure of a clinical trial, multiple case series have described encouraging results, especially with the introduction of improved surgical protocols. Recent evidence further suggests that tractography targeting and intraoperative exposure to stimulation enhances early antidepressant effects that further evolve with ongoing chronic DBS. Accelerating treatment gains is critical to the care of this at-risk population, and identification of intraoperative electrophysiological biomarkers of early antidepressant effects will help guide future treatment protocols. Eight patients underwent intraoperative electrophysiological recording when bilateral DBS leads were implanted in the SCC using a connectomic approach at the site previously shown to optimize 6-month treatment outcomes. A machine learning classification method was used to discriminate between intracranial local field potentials (LFPs) recorded at baseline (stimulation-naïve) and after the first exposure to SCC DBS during surgical procedures. Spectral inputs (theta, 4–8 Hz; alpha, 9–12 Hz; beta, 13–30 Hz) to the model were then evaluated for importance to classifier success and tested as predictors of the antidepressant response. A decline in depression scores by 45.6% was observed after 1 week and this early antidepressant response correlated with a decrease in SCC LFP beta power, which most contributed to classifier success. Intraoperative exposure to therapeutic stimulation may result in an acute decrease in symptoms of depression following SCC DBS surgery. The correlation of symptom improvement with an intraoperative reduction in SCC beta power suggests this electrophysiological finding as a biomarker for treatment optimization.
Switched Aβ43 generation in familial Alzheimer’s disease with presenilin 1 mutation
Authors: Kakuda Nobuto , Takami Mako , Okochi Masayasu , Kasuga Kensaku , Ihara Yasuo , Ikeuchi Takeshi
Published: November 2021

Presenilin (PS) with a genetic mutation generates abundant β-amyloid protein (Aβ) 43. Senile plaques are formed by Aβ43 in the cerebral parenchyma together with Aβ42 at middle ages. These brains cause the early onset of Alzheimer’s disease (AD), which is known as familial Alzheimer’s disease (FAD). Based on the stepwise processing model of Aβ generation by γ-secretase, we reassessed the levels of Aβs in the cerebrospinal fluid (CSF) of FAD participants. While low levels of Aβ38, Aβ40, and Aβ42 were generated in the CSF of FAD participants, the levels of Aβ43 were unchanged in some of them compared with other participants. We sought to investigate why the level of Aβ43 was unchanged in FAD participants. These characteristics of Aβ generation were observed in the γ-secretase assay in vitro using cells, which express FAD mutations in PS1. Aβ38 and Aβ40 generation from their precursors, Aβ42 and Aβ43, was decreased in PS1 mutants compared with wild-type (WT) PS1, as observed in the CSF. Both the ratios of Aβ38/Aβ42 and Aβ40/Aβ43 in PS1 mutants were lower than those in the WT. However, the ratio of Aβ43/amyloid precursor protein intracellular domain (AICD) increased in the PS1 mutants in an onset age dependency, while other Aβ/AICD ratios were decreased or unchanged. Importantly, liquid chromatography–mass spectrometry found that the generation of Aβ43 was stimulated from Aβ48 in PS1 mutants. This result indicates that PS1 mutants switched the Aβ43 generating line, which reflects the level of Aβ43 in the CSF and forming senile plaques.
The sooner the better: clinical and neural correlates of impulsive choice in Tourette disorder
Authors: Atkinson-Clement Cyril , de Liege Astrid , Klein Yanica , Beranger Benoit , Valabregue Romain , Delorme Cecile , Roze Emmanuel , Fernandez-Egea Emilio , Hartmann Andreas , Robbins Trevor W. , Worbe Yulia
Published: November 2021

Reward sensitivity has been suggested as one of the central pathophysiological mechanisms in Tourette disorder. However, the subjective valuation of a reward by introduction of delay has received little attention in Tourette disorder, even though it has been suggested as a trans-diagnostic feature of numerous neuropsychiatric disorders. We aimed to assess delay discounting in Tourette disorder and to identify its brain functional correlates. We evaluated delayed discounting and its brain functional correlates in a large group of 54 Tourette disorder patients and 31 healthy controls using a data-driven approach. We identified a subgroup of 29 patients with steeper reward discounting, characterised by a higher burden of impulse-control disorders and a higher level of general impulsivity compared to patients with normal behavioural performance or to controls. Reward discounting was underpinned by resting-state activity of a network comprising the orbito-frontal, cingulate, pre-supplementary motor area, temporal and insular cortices, as well as ventral striatum and hippocampus. Within this network, (i) lower connectivity of pre-supplementary motor area with ventral striatum predicted a higher impulsivity and a steeper reward discounting and (ii) a greater connectivity of pre-supplementary motor area with anterior insular cortex predicted steeper reward discounting and more severe tics. Overall, our results highlight the heterogeneity of the delayed reward processing in Tourette disorder, with steeper reward discounting being a marker of burden in impulsivity and impulse control disorders, and the pre-supplementary motor area being a hub region for the delay discounting, impulsivity and tic severity.
RETRACTED ARTICLE: Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs
Authors: Yarmarkovich Mark , Marshall Quinlen F. , Warrington John M. , Premaratne Rasika , Farrel Alvin , Groff David , Li Wei , di Marco Moreno , Runbeck Erin , Truong Hau , Toor Jugmohit S. , Tripathi Sarvind , Nguyen Son , Shen Helena , Noel Tiffany , Church Nicole L. , Weiner Amber , Kendsersky Nathan , Martinez Dan , Weisberg Rebecca , Christie Molly , Eisenlohr Laurence , Bosse Kristopher R. , Dimitrov Dimiter S. , Stevanovic Stefan , Sgourakis Nikolaos G. , Kiefel Ben R. , Maris John M.
Published: November 2021

The majority of oncogenic drivers are intracellular proteins, thus constraining their immunotherapeutic targeting to mutated peptides (neoantigens) presented by individual human leukocyte antigen (HLA) allotypes1. However, most cancers have a modest mutational burden that is insufficient to generate responses using neoantigen-based therapies2,3. Neuroblastoma is a paediatric cancer that harbours few mutations and is instead driven by epigenetically deregulated transcriptional networks4. Here we show that the neuroblastoma immunopeptidome is enriched with peptides derived from proteins that are essential for tumourigenesis and focus on targeting the unmutated peptide QYNPIRTTF, discovered on HLA-A*24:02, which is derived from the neuroblastoma dependency gene and master transcriptional regulator PHOX2B. To target QYNPIRTTF, we developed peptide-centric chimeric antigen receptors (CARs) using a counter-panning strategy with predicted potentially cross-reactive peptides. We further hypothesized that peptide-centric CARs could recognize peptides on additional HLA allotypes when presented in a similar manner. Informed by computational modelling, we showed that PHOX2B peptide-centric CARs also recognize QYNPIRTTF presented by HLA-A*23:01 and the highly divergent HLA-B*14:02. Finally, we demonstrated potent and specific killing of neuroblastoma cells expressing these HLAs in vitro and complete tumour regression in mice. These data suggest that peptide-centric CARs have the potential to vastly expand the pool of immunotherapeutic targets to include non-immunogenic intracellular oncoproteins and widen the population of patients who would benefit from such therapy by breaking conventional HLA restriction.
Terrestrial-type nitrogen-fixing symbiosis between seagrass and a marine bacterium
Authors: Mohr Wiebke , Lehnen Nadine , Ahmerkamp Soeren , Marchant Hannah K. , Graf Jon S. , Tschitschko Bernhard , Yilmaz Pelin , Littmann Sten , Gruber-Vodicka Harald , Leisch Nikolaus , Weber Miriam , Lott Christian , Schubert Carsten J. , Milucka Jana , Kuypers Marcel M. M.
Published: November 2021

Symbiotic N2-fixing microorganisms have a crucial role in the assimilation of nitrogen by eukaryotes in nitrogen-limited environments1–3. Particularly among land plants, N2-fixing symbionts occur in a variety of distantly related plant lineages and often involve an intimate association between host and symbiont2,4. Descriptions of such intimate symbioses are lacking for seagrasses, which evolved around 100 million years ago from terrestrial flowering plants that migrated back to the sea5. Here we describe an N2-fixing symbiont, ‘Candidatus Celerinatantimonas neptuna’, that lives inside seagrass root tissue, where it provides ammonia and amino acids to its host in exchange for sugars. As such, this symbiosis is reminiscent of terrestrial N2-fixing plant symbioses. The symbiosis between Ca. C. neptuna and its host Posidonia oceanica enables highly productive seagrass meadows to thrive in the nitrogen-limited Mediterranean Sea. Relatives of Ca. C. neptuna occur worldwide in coastal ecosystems, in which they may form similar symbioses with other seagrasses and saltmarsh plants. Just like N2-fixing microorganisms might have aided the colonization of nitrogen-poor soils by early land plants6, the ancestors of Ca. C. neptuna and its relatives probably enabled flowering plants to invade nitrogen-poor marine habitats, where they formed extremely efficient blue carbon ecosystems7.
Robotic fabrication of high-quality lamellae for aberration-corrected transmission electron microscopy
Authors: Tsurusawa Hideyo , Nakanishi Nobuto , Kawano Kayoko , Chen Yiqiang , Dutka Mikhail , Van Leer Brandon , Mizoguchi Teruyasu
Published: November 2021

Aberration-corrected scanning transmission electron microscopy (STEM) is widely used for atomic-level imaging of materials but severely requires damage-free and thin samples (lamellae). So far, the preparation of the high-quality lamella from a bulk largely depends on manual processes by a skilled operator. This limits the throughput and repeatability of aberration-corrected STEM experiments. Here, inspired by the recent successes of “robot scientists”, we demonstrate robotic fabrication of high-quality lamellae by focused-ion-beam (FIB) with automation software. First, we show that the robotic FIB can prepare lamellae with a high success rate, where the FIB system automatically controls rough-milling, lift-out, and final-thinning processes. Then, we systematically optimized the FIB parameters of the final-thinning process for single crystal Si. The optimized Si lamellae were evaluated by aberration-corrected STEM, showing atomic-level images with 55 pm resolution and quantitative repeatability of the spatial resolution and lamella thickness. We also demonstrate robotic fabrication of high-quality lamellae of SrTiO3 and sapphire, suggesting that the robotic FIB system may be applicable for a wide range of materials. The throughput of the robotic fabrication was typically an hour per lamella. Our robotic FIB will pave the way for the operator-free, high-throughput, and repeatable fabrication of the high-quality lamellae for aberration-corrected STEM.
Sitosterol and glucosylceramide cooperative transversal and lateral uneven distribution in plant membranes
Authors: Rondelli V. , Koutsioubas A. , Pršić J. , Deboever E. , Crowet J. M. , Lins L. , Deleu M.
Published: November 2021

The properties of biomembranes depend on the presence, local structure and relative distribution assumed by the thousands of components it is made of. As for animal cells, plant membranes have been demonstrated to be organized in subdomains with different persistence lengths and times. In plant cells, sitosterol has been demonstrated to confer to phospholipid membranes a more ordered structure while among lipids, glycosphingolipids are claimed to form rafts where they tightly pack with sterols. Glucosylceramides are glycosphingolipids involved in plant signalling and are essential for viability of cells and whole plant. The glucosylceramide-sitosterol structural coupling within PLPC membranes is here investigated by Langmuir films, in silico simulations and neutron reflectometry, unveiling that a strong direct interaction between the two molecules exists and governs their lateral and transversal distribution within membrane leaflets. The understanding of the driving forces governing specific molecules clustering and segregation in subdomains, such as glucosylceramide and sitosterol, have an impact on the mechanical properties of biomembranes and could reflect in the other membrane molecules partitioning and activity.
Optimally-weighted non-linear beamformer for conventional focused beam ultrasound imaging systems
Authors: Vayyeti Anudeep , Thittai Arun K.
Published: November 2021

A novel non-linear beamforming method, namely, filtered delay optimally-weighted multiply and sum (F-DowMAS) beamforming is reported for conventional focused beamforming (CFB) technique. The performance of F-DowMAS was compared against delay and sum (DAS), filtered delay multiply and sum (F-DMAS), filtered delay weight multiply and sum (F-DwMAS) and filter delay Euclidian weighted multiply and sum (F-DewMAS) methods. Notably, in the proposed method the optimal adaptive weights are computed for each imaging point to compensate for the effects due to spatial variations in beam pattern in CFB technique. F-DowMAS, F-DMAS, and DAS were compared in terms of the resulting image quality metrics, Lateral resolution (LR), axial resolution (AR), contrast ratio (CR) and contrast-to-noise ratio (CNR), estimated from experiments on a commercially available tissue-mimicking phantom. The results demonstrate that F-DowMAS improved the AR by 57.04% and 46.95%, LR by 58.21% and 53.40%, CR by 67.35% and 39.25%, and CNR by 44.04% and 30.57% compared to those obtained using DAS and F-DMAS, respectively. Thus, it can be concluded that the newly proposed F-DowMAS outperforms DAS and F-DMAS. As an aside, we also show that the optimal weighting strategy can be extended to benefit DAS.
Infection induced SARS-CoV-2 seroprevalence and heterogeneity of antibody responses in a general population cohort study in Catalonia Spain
Authors: Karachaliou Marianna , Moncunill Gemma , Espinosa Ana , Castaño-Vinyals Gemma , Jiménez Alfons , Vidal Marta , Santano Rebeca , Barrios Diana , Puyol Laura , Carreras Anna , Mayer Leonie , Rubio Rocío , Cortés Beatriz , Pleguezuelos Vanessa , O’Callaghan-Gordo Cristina , Fossati Serena , Rivas Ioar , Casabonne Delphine , Vrijheid Martine , Izquierdo Luis , Aguilar Ruth , Basagaña Xavier , Garcia-Aymerich Judith , de Cid Rafael , Dobaño Carlota , Kogevinas Manolis
Published: November 2021

Sparse data exist on the complex natural immunity to SARS-CoV-2 at the population level. We applied a well-validated multiplex serology test in 5000 participants of a general population study in Catalonia in blood samples collected from end June to mid November 2020. Based on responses to fifteen isotype-antigen combinations, we detected a seroprevalence of 18.1% in adults (n = 4740), and modeled extrapolation to the general population of Catalonia indicated a 15.3% seroprevalence. Antibodies persisted up to 9 months after infection. Immune profiling of infected individuals revealed that with increasing severity of infection (asymptomatic, 1–3 symptoms, ≥ 4 symptoms, admitted to hospital/ICU), seroresponses were more robust and rich with a shift towards IgG over IgA and anti-spike over anti-nucleocapsid responses. Among seropositive participants, lower antibody levels were observed for those ≥ 60 years vs < 60 years old and smokers vs non-smokers. Overweight/obese participants vs normal weight had higher antibody levels. Adolescents (13–15 years old) (n = 260) showed a seroprevalence of 11.5%, were less likely to be tested seropositive compared to their parents and had dominant anti-spike rather than anti-nucleocapsid IgG responses. Our study provides an unbiased estimate of SARS-CoV-2 seroprevalence in Catalonia and new evidence on the durability and heterogeneity of post-infection immunity.
Feedback between bottom-up and top-down control of stream biofilm mediated through eutrophication effects on grazer growth
Authors: Iannino Alessandra , Fink Patrick , Weitere Markus
Published: November 2021

Algal biofilms in streams are simultaneously controlled by light and nutrient availability (bottom-up control) and by grazing activity (top-down control). In addition to promoting algal growth, light and nutrients also determine the nutritional quality of algae for grazers. While short-term experiments have shown that grazers increase consumption rates of nutrient-poor algae due to compensatory feeding, nutrient limitation in the long run can constrain grazer growth and hence limit the strength of grazing activity. In this study, we tested the effects of light and phosphorus availability on grazer growth and thus on the long-term control of algal biomass. At the end of the experiment, algal biomass was significantly affected by light, phosphorus and grazing, but the interactive effects of the three factors significantly changed over time. At both high light and phosphorus supply, grazing did not initially reduce algal biomass, but the effect of grazing became stronger in the final three weeks of the experiment. Snail growth was enhanced by light, rather than phosphorus, suggesting that algal quantity rather than quality was the main limiting factor for grazer growth. Our results highlight the role of feedback effects and the importance of long-term experiments in the study of foodweb interactions.
National cohort and meteorological data based nested case–control study on the association between air pollution exposure and thyroid cancer
Authors: Park Sung Joon , Min Chanyang , Yoo Dae Myoung , Choi Hyo Geun
Published: November 2021

The objective of this study was to evaluate the influence of exposure to meteorological conditions, including air pollution, on thyroid cancer. A nested case–control study was conducted utilizing 4632 patients with thyroid cancer and 18,528 control subjects who were matched at a 1:4 ratio by age group, sex, income, and region of residence. Korean National Health Insurance Service-Health Screening Cohort data from 2002 to 2015 were used. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated for thyroid cancer correlated with meteorological and air pollution exposure over a moving average of 3 years before the index dates. For all participants, the adjusted ORs associated with relative humidity (1.01, 95% CI 1.00–1.03, P value = 0.023), ambient atmospheric pressure (1.02, 95% CI 1.01–1.03, P value < 0.001), and sunshine duration (1.17, 95% CI 1.04–1.31, P value = 0.007) indicated correlations with the occurrence of thyroid cancer; however, these results were inconsistent in the subgroup analyses. Overall, exposure to nitrogen dioxide (NO2) (1.33, 95% CI 1.24–1.43, P value < 0.001) and particulate matter (PM10) (0.64, 95% CI 0.60–0.69, P value < 0.001) were related to thyroid cancer. These relationships persisted in the subgroup analyses. In conclusion, thyroid cancer occurrence was positively associated with NO2 exposure and negatively associated with PM10 exposure.
SARS-CoV-2 IgG seropositivity in a cohort of 449 non-hospitalized individuals during Spanish COVID-19 lockdown
Authors: Martínez Patricia Torres , García Paula Diaque , Salas María Rubio , Sánchez Raquel Rodríguez , Avendaño-Ortíz José , Guerrero-Monjo Sandra , García Felipe , Llamas Miguel Ángel , López-Collazo Eduardo , Saz-Leal Paula , del Fresno Carlos
Published: November 2021

The Coronavirus Disease of 2019 (COVID-19) pandemic caused by SARS-CoV-2 led the Spanish government to impose a national lockdown in an attempt to control the spread of the infection. Mobility restrictions and the requirement of a medical prescription for serological testing for COVID-19 were included among the control measures. Under this scenario, between April 15th and June 15th, 2020, we performed an observational study including 449 individuals allowed to be tested according to the governmental restrictions, i.e. fulfilling the following prescription requirements: manifestation of COVID-19-compatible symptoms, contact with a confirmed COVID-19 patient, or employment as an essential worker, including health care workers, firefighters and public safety personnel such as police. Importantly, a relevant feature of the studied cohort was that none of the participants had been hospitalized. We analyzed SARS-CoV-2 IgG seropositivity in this specific cohort, uncovering intrinsic features of great demographic interest. The overall rate of IgG seropositivity was 33.69% (95% CI: 29.27–38.21). This frequency was comparable among the different participant occupations. A RT-PCR positive test, contact with a household member previously tested positive and the presence of COVID-19-compatible symptoms were positively associated with IgG + results. Among these symptoms, ageusia/anosmia was positively and independently associated with SARS-CoV-2 IgG seropositivity, while odynophagia was inversely associated. However, fever, ageusia/anosmia and asthenia were the most frequent symptoms described by IgG + subjects. Therefore, our data illustrate how specific cohorts display particular characteristics that should be taken into account when studying population-wide SARS-CoV-2 seroprevalence and key defining symptoms of COVID-19.
Prognostic value of different N1 lymph node zones in pN1M0 non-small cell lung cancer: a systematic review and meta-analysis
Authors: Hu Fengling , Lin Chutong , Chu Hongling , Ren Peng , Wang Jingdi , Ma Shaohua
Published: November 2021

The IASLC lymph node map grouped the lymph node stations into “zones” for prognostic analyses. In the N1 lymph nodes group, N1 nodes are divided into the Hilar/Interlobar zone (N1h) and Peripheral zone (N1p). There is no consensus on the different prognostic values of N1 lymph nodes in N1h and N1p. Therefore, we conducted a systematic review and meta-analysis to assess the survival difference between N1h and N1p in patients of pN1M0 NSCLC. Medline, the Cochrane Library, Embase, and the Web of science were systematically searched to identify relevant studies published up to April 4th, 2020. A retrospective and prospective cohort study comparing N1h versus N1p to the pN1M0 NSCLC was included. Hazard ratios (HRs) for OS were aggregated according to a fixed or random-effect model. Ten publications for 1946 patients of pN1M0 NSCLC were included for the meta-analysis.The 5-year OS was lower for patients with N1h (HR: 1.67, 95% CI 1.44–1.94; P < 0.001). The pooled 5-year OS in N1h and N1p were 40% and 56%, respectively. The patients in pN1M0 NSCLC have different survival according to different N1 lymph node zones involvement: patients with N1p metastasis have a better prognosis than those with N1h metastasis.