bluuebunny/crossref_metadata_2025 huggingface dataset:
Computational Particle Fluid Dynamics Simulation on Combustion Characteristics of Blended Fuels of Coal, Biomass, and Oil Sludge in a 130 t h−1 Circulating Fluidized Bed Boiler
Authors: Wang Yang , Chen Xiangyu , Xu Liping , Ma Mingwei , Huang Xiaole , Han Feng , Zhou Yong , Du Chen , Da Yaodong , Deng Lei
Published: December 2023

In this study, the co-combustion of coal and biomass, and the tri-combustion of coal, biomass, and oil sludge in a 130 t h−1 circulating fluidized bed (CFB) boiler are investigated via the computational particle fluid dynamics (CPFD) approach. Furthermore, the effect of biomass feeding position is also comprehensively evaluated. The results show that for the co-combustion of coal and biomass, the O2 mole fraction at the furnace outlet rises from 0.0541 to 0.0640 as the biomass blending ratio enhances from 40% to 100%, while the CO2 mole fraction reduces from 0.1357 to 0.1267. The mole fraction of NOx and SO2 at the furnace outlet decreases from 4.5867 × 10−5 to 3.9096 × 10−5 and 2.8253 × 10−4 to 4.6635 × 10−5, respectively. For the tri-combustion of three fuels, the average NOx mole fraction initially grows quickly and then declines gradually, ranging from 4.1173 × 10−5 to 4.2556 × 10−5. The mole fraction of SO2 at the furnace outlet increases from 3.5176 × 10−4 to 4.7043 × 10−4 when the ratio of oil sludge rises from 10% to 20%. The uniformity of temperature and gas components distribution is “new inlet > secondary air inlet > feed inlet”. As for the three inlet positions, the mole fractions of NOx at the furnace outlet are between 3.9096 × 10−5 and 5.1537 × 10−5, while those for SO2 are between 2.5978 × 10−4 and 2.5278 × 10−4.
Determination of the Optimal Level of Reactive Power Compensation That Minimizes the Costs of Losses in Distribution Networks
Authors: Andruszkiewicz Jerzy , Lorenc Józef , Weychan Agnieszka
Published: December 2023

The objective of the presented paper is to verify economically justified levels of reactive energy compensation in the distribution network in the new market conditions, including the extensive use of smart metering systems, new types of load, or distributed generation. The proposed methodology is based on the minimization of annual costs of losses caused by the flow of reactive energy to the supplied loads through the equivalent resistance of the distribution system determined on the basis of statistical energy losses in this network. The costs of losses are compared to the costs of using compensating devices expressed by the levelized costs of reactive energy generation. The results are the relations describing the optimal annual average value of the tgφ factor to be maintained by customers to optimize the cost of loss of the distribution network caused by reactive energy flows. The dependence of the optimal tgφ value on the analyzed load and network parameters is also discussed. The resulting optimal tgφ levels should be considered in the tariffication process of services offered by distribution system operators to improve capacity and limit the costs of power network operation due to reactive energy transmission.
Channel Access in Wireless Smart Grid Networks Operating under ETSI Frame-Based Equipment Rules
Authors: Karcz Marcin , Szott Szymon
Published: December 2023

Smart grid operators seeking to extend their wireless network capacity can use unlicensed bands. However, devices in these shared bands must follow rules such as Listen Before Talk (LBT), standardized by ETSI. In this paper, we focus on the performance of the frame-based equipment (FBE) version of LBT channel access. We design, implement, and validate a fully functional FBE channel access simulator. Next, we conduct an extensive performance analysis of the FBE variants encountered in the literature, focusing on channel efficiency and fairness in upper-bound and coexistence scenarios. Our study leads to several conclusions about the operation of FBE-based devices, including the need for proper configuration of channel access parameters to ensure fairness and optimal performance. We also observe generally poor coexistence among FBE variants: the highest Jain’s fairness index was only 0.88, with an average normalized channel efficiency of 0.76. Therefore, we identify several open research areas in the field, such as the need for further development of parameter adaptation algorithms, the deployment of an external controller to update channel access parameters, and new FBE designs with better coexistence qualities.
Mountain Taiga in a Warming Climate: Contrast of Siberian Pine Growth along an Elevation Gradient
Authors: Kharuk Viacheslav I. , Petrov Il’ya A. , Golyukov Alexey S. , Im Sergei T. , Shushpanov Alexander S.
Published: December 2023

The growth and survival of trees in the Siberian Mountains are experiencing a strong influence on climate warming. We analyzed Siberian pine (SP, Pinus sibirica) growth within the treeline ecotone in high (>1000 m) and low (<900 m) lands. We used ground surveys, dendrochronology, and climate variable data analysis. We found a contrasting response of SP growth with increasing air temperature and moisture parameters along the elevation gradient. In the treeline ecotone and highlands, the tree’s growth has been increasing since warming onset in the 1970s, whereas in the lowlands, the initial growth increase switched to a growth drop since the beginning of the 2000s, with a consequent partial mortality of the Siberian pine forest caused by warming-driven water stress in combination with bark borers’ attacks. This mortality suggests the retraction of the Siberian pine range in the lowlands of the Siberian Mountains. The projected drought increase will likely lead to the substitution of Siberian pine with drought-tolerant species. The tree’s growth index (GI) dependence on air temperature and moisture variables includes two phases. In the first phase (since the warming onset in the 1970s), the trees’ GI was positively correlated with elevated temperature, whereas correlations with precipitation and soil moisture were negative. During the second phase (since the increase in warming in the 2000s), negative correlations between the GI and moisture variables switched to positive ones. The correlations of the GI with air temperature switched from positive to mostly insignificant. The wind’s influence on the trees’ growth changed from negative to insignificant since the 2000s within all elevation belts. Afforestation within the areas of Siberian pine mortality should not be based on the planting of Siberian pine but on drought-tolerant species such as larch (Larix sibirica) and Scots pine (Pinus sylvestris).
Genome-Wide Identification, Expression and Interaction Analyses of PP2C Family Genes in Chenopodium quinoa
Authors: Yang Dongdong , Zhang Xia , Cao Meng , Yin Lu , Gao Aihong , An Kexin , Gao Songmei , Guo Shanli , Yin Haibo
Published: December 2023

Plant protein phosphatase 2Cs (PP2Cs) function as inhibitors in protein kinase cascades involved in various processes and are crucial participants in both plant development and signaling pathways activated by abiotic stress. In this study, a genome-wide study was conducted on the CqPP2C gene family. A total of putative 117 CqPP2C genes were identified. Comprehensive analyses of physicochemical properties, chromosome localization and subcellular localization were conducted. According to phylogenetic analysis, CqPP2Cs were divided into 13 subfamilies. CqPP2Cs in the same subfamily had similar gene structures, and conserved motifs and all the CqPP2C proteins had the type 2C phosphatase domains. The expansion of CqPP2Cs through gene duplication was primarily driven by segmental duplication, and all duplicated CqPP2Cs underwent evolutionary changes guided by purifying selection. The expression of CqPP2Cs in various tissues under different abiotic stresses was analyzed using RNA-seq data. The findings indicated that CqPP2C genes played a role in regulating both the developmental processes and stress responses of quinoa. Real-time quantitative reverse transcription PCR (qRT-PCR) analysis of six CqPP2C genes in subfamily A revealed that they were up-regulated or down-regulated under salt and drought treatments. Furthermore, the results of yeast two-hybrid assays revealed that subfamily A CqPP2Cs interacted not only with subclass III CqSnRK2s but also with subclass II CqSnRK2s. Subfamily A CqPP2Cs could interact with CqSnRK2s in different combinations and intensities in a variety of biological processes and biological threats. Overall, our results will be useful for understanding the functions of CqPP2C in regulating ABA signals and responding to abiotic stress.
Effect of Different Approaches to Antimicrobial Therapy with Cefmetazole and Meropenem on the Time to Defervescence in Non-Severe Extended-Spectrum β-Lactamase-Producing Escherichia coli Bacteremia
Authors: Hoshi Takanobu , Fujii Satoshi , Watanabe Kei , Fukumura Yuta , Miyazaki Koji , Takahashi Madoka , Taniguchi Sakae , Kimura Shingo , Saito Arisa , Wada Naoki , Saijo Masaji , Yamada Kazunori , Iwayama Kuninori , Itaya Marie , Sato Hideki
Published: December 2023

Carbapenems are antimicrobial agents commonly used to treat extended-spectrum β-lactamase (ESBL)-producing bacteria. Although cefmetazole (CMZ) is considered effective for ESBL-producing Escherichia coli (ESBL-EC) bacteremia, previous studies showed its limitations, including the influence of the initial antimicrobial agent. Here, we examined the effects of different approaches to antimicrobial therapy with CMZ and meropenem (MEPM) on the time to defervescence in ESBL-EC bacteremia. Notably, the influence of previous antimicrobial agents was excluded. Inpatients with ESBL-EC detected in blood cultures between April 2018 and March 2023 were included and assigned to CMZ (n = 14), MEPM (n = 8), de-escalation to CMZ (dCMZ; n = 9), or escalation to MEPM (eMEPM; n = 11) groups. The median time to defervescence was 3.5, 1.0, 2.0, and 4.0 days in the CMZ, MEPM, dCMZ, and eMEPM groups, respectively, with no significant differences. Cox proportional hazards analysis showed a significant difference in the hazard ratio (95% confidence interval) of 0.378 (0.145–0.984) for the time to defervescence with CMZ versus MEPM (p = 0.046). The extent of a delayed time to defervescence is greater with early CMZ administration than with MEPM administration in patients with non-severe ESBL-EC bacteremia.
Unpacking the Public Health Triad of Social Inequality in Health, Health Literacy, and Quality of Life—A Scoping Review of Research Characteristics
Authors: Holmen Heidi , Flølo Tone , Tørris Christine , Løyland Borghild , Almendingen Kari , Bjørnnes Ann Kristin , Albertini Früh Elena , Grov Ellen Karine , Helseth Sølvi , Kvarme Lisbeth Gravdal , Malambo Rosah , Misvær Nina , Rasalingam Anurajee , Riiser Kirsti , Sandbekken Ida Hellum , Schippert Ana Carla , Sparboe-Nilsen Bente , Sundar Turid Kristin Bigum , Sæterstrand Torill , Utne Inger , Valla Lisbeth , Winger Anette , Torbjørnsen Astrid
Published: December 2023

Social inequalities in health, health literacy, and quality of life serve as distinct public health indicators, but it remains unclear how and to what extent they are applied and combined in the literature. Thus, the characteristics of the research have yet to be established, and we aim to identify and describe the characteristics of research that intersects social inequality in health, health literacy, and quality of life. We conducted a scoping review with systematic searches in ten databases. Studies applying any design in any population were eligible if social inequality in health, health literacy, and quality of life were combined. Citations were independently screened using Covidence. The search yielded 4111 citations, with 73 eligible reports. The reviewed research was mostly quantitative and aimed at patient populations in a community setting, with a scarcity of reports specifically defining and assessing social inequality in health, health literacy, and quality of life, and with only 2/73 citations providing a definition for all three. The published research combining social inequality in health, health literacy, and quality of life is heterogeneous regarding research designs, populations, contexts, and geography, where social inequality appears as a contextualizing variable.
Improving the Maternity Care Safety Net: Establishing Maternal Mortality Surveillance for Non-Obstetric Providers and Institutions
Authors: Combellick Joan L. , Basile Ibrahim Bridget , Esmaeili Aryan , Phibbs Ciaran S. , Johnson Amanda M. , Patton Elizabeth Winston , Manzo Laura , Haskell Sally G.
Published: December 2023

The siloed nature of maternity care has been noted as a system-level factor negatively impacting maternal outcomes. Veterans Health Administration (VA) provides multi-specialty healthcare before, during, and after pregnancy but purchases obstetric care from community providers. VA providers may be unaware of perinatal complications, while community-based maternity care providers may be unaware of upstream factors affecting the pregnancy. To optimize maternal outcomes, the VA has initiated a system-level surveillance and review process designed to improve non-obstetric care for veterans experiencing a pregnancy. This quality improvement project aimed to describe the VA-based maternal mortality review process and to report maternal mortality (pregnancy-related death up to 42 days postpartum) and pregnancy-associated mortality (death from any cause up to 1 year postpartum) among veterans who use VA maternity care benefits. Pregnancies and pregnancy-associated deaths between fiscal year (FY) 2011–2020 were identified from national VA databases. All deaths underwent individual chart review and abstraction that focused on multi-specialty care received at the VA in the year prior to pregnancy until the time of death. Thirty-two pregnancy-associated deaths were confirmed among 39,720 pregnancies (PAMR = 80.6 per 100,000 live births). Fifty percent of deaths occurred among individuals who had experienced adverse social determinants of health. Mental health conditions affected 81%. Half (n = 16, 50%) of all deaths occurred in the late postpartum period (43–365 days postpartum) after maternity care had ended. More than half of these late postpartum deaths (n = 9, 56.2%) were related to suicide, homicide, or overdose. Integration of care delivered during the perinatal period (pregnancy through postpartum) from primary, mental health, emergency, and specialty care providers may be enhanced through a system-based approach to pregnancy-associated death surveillance and review. This quality improvement project has implications for all healthcare settings where coordination between obstetric and non-obstetric providers is needed.
Urban Violence, Migration and Alcohol, Tobacco, and Marijuana Use among Transnational Students in Northern Mexico
Authors: García-Pérez Hilda , Kulis Stephen S. , Marsiglia Flavio F. , Estabrooks Paul A.
Published: December 2023

This article reports on the findings of a study of the relationship between transnational experiences in the United States (US) and the use of alcohol, tobacco, and marijuana among 7th grade students (n = 1418). The study was guided by a cross-national framework for research on immigrant health and assessed the accumulation of risk factors for transnational adolescents. Data came from a survey conducted in 2017 in Nogales, Mexico. In this study, the last 30-day prevalence of use of alcohol, tobacco, and marijuana among students was 21.7%, 8.3%, and 2.4%, respectively. Most students were born in Nogales (69.6%), while 10.5% were born in the US, 7.5% attended school in the US, and 3.6% engaged in health-related risk behaviors while living in or visiting the US. Students with transnational experiences, such as attending school in the US, reported the highest 30-day prevalence of tobacco (13.3%) and marijuana (9.5%) use. After adjusting for family, school, access to substances and neighborhood violence variables, students who engaged in health-related risk behavior in the US had significantly increased odds of alcohol and marijuana use while later attending school in Mexico. The article discusses the findings from a prevention science perspective and provides implications for policy, practice, and future research on the Mexico-US border region.
Cis-Regulatory Elements in Mammals
Authors: Liu Xingyu , Chen Mengjie , Qu Xiuwen , Liu Wenjing , Dou Yuting , Liu Qingyou , Shi Deshun , Jiang Mingsheng , Li Hui
Published: December 2023

In cis-regulatory elements, enhancers and promoters with complex molecular interactions are used to coordinate gene transcription through physical proximity and chemical modifications. These processes subsequently influence the phenotypic characteristics of an organism. An in-depth exploration of enhancers and promoters can substantially enhance our understanding of gene regulatory networks, shedding new light on mammalian development, evolution and disease pathways. In this review, we provide a comprehensive overview of the intrinsic structural attributes, detection methodologies as well as the operational mechanisms of enhancers and promoters, coupled with the relevant novel and innovative investigative techniques used to explore their actions. We further elucidated the state-of-the-art research on the roles of enhancers and promoters in the realms of mammalian development, evolution and disease, and we conclude with forward-looking insights into prospective research avenues.
Nucleotide, Phospholipid, and Kynurenine Metabolites Are Robustly Associated with COVID-19 Severity and Time of Plasma Sample Collection in a Prospective Cohort Study
Authors: Chatelaine Haley A. S. , Chen Yulu , Braisted John , Chu Su H. , Chen Qingwen , Stav Meryl , Begum Sofina , Diray-Arce Joann , Sanjak Jaleal , Huang Mengna , Lasky-Su Jessica , Mathé Ewy A.
Published: December 2023

Understanding the molecular underpinnings of disease severity and progression in human studies is necessary to develop metabolism-related preventative strategies for severe COVID-19. Metabolites and metabolic pathways that predispose individuals to severe disease are not well understood. In this study, we generated comprehensive plasma metabolomic profiles in >550 patients from the Longitudinal EMR and Omics COVID-19 Cohort. Samples were collected before (n = 441), during (n = 86), and after (n = 82) COVID-19 diagnosis, representing 555 distinct patients, most of which had single timepoints. Regression models adjusted for demographics, risk factors, and comorbidities, were used to determine metabolites associated with predisposition to and/or persistent effects of COVID-19 severity, and metabolite changes that were transient/lingering over the disease course. Sphingolipids/phospholipids were negatively associated with severity and exhibited lingering elevations after disease, while modified nucleotides were positively associated with severity and had lingering decreases after disease. Cytidine and uridine metabolites, which were positively and negatively associated with COVID-19 severity, respectively, were acutely elevated, reflecting the particular importance of pyrimidine metabolism in active COVID-19. This is the first large metabolomics study using COVID-19 plasma samples before, during, and/or after disease. Our results lay the groundwork for identifying putative biomarkers and preventive strategies for severe COVID-19.
Icariin Improves Stress Resistance and Extends Lifespan in Caenorhabditis elegans through hsf-1 and daf-2-Driven Hormesis
Authors: Todorova Monika N. , Savova Martina S. , Mihaylova Liliya V. , Georgiev Milen I.
Published: December 2023

Aging presents an increasingly significant challenge globally, driven by the growing proportion of individuals aged 60 and older. Currently, there is substantial research interest in pro-longevity interventions that target pivotal signaling pathways, aiming not only to extend lifespan but also to enhance healthspan. One particularly promising approach involves inducing a hormetic response through the utilization of natural compounds defined as hormetins. Various studies have introduced the flavonoid icariin as beneficial for age-related diseases such as cardiovascular and neurodegenerative conditions. To validate its potential pro-longevity properties, we employed Caenorhabditis elegans as an experimental platform. The accumulated results suggest that icariin extends the lifespan of C. elegans through modulation of the DAF-2, corresponding to the insulin/IGF-1 signaling pathway in humans. Additionally, we identified increased resistance to heat and oxidative stress, modulation of lipid metabolism, improved late-life healthspan, and an extended lifespan upon icariin treatment. Consequently, a model mechanism of action was provided for icariin that involves the modulation of various players within the stress-response network. Collectively, the obtained data reveal that icariin is a potential hormetic agent with geroprotective properties that merits future developments.
Alpha-Synuclein Contribution to Neuronal and Glial Damage in Parkinson’s Disease
Authors: Saramowicz Kamil , Siwecka Natalia , Galita Grzegorz , Kucharska-Lusina Aleksandra , Rozpędek-Kamińska Wioletta , Majsterek Ireneusz
Published: December 2023

Parkinson’s disease (PD) is a complex neurodegenerative disease characterized by the progressive loss of dopaminergic neurons in the substantia nigra and the widespread accumulation of alpha-synuclein (αSyn) protein aggregates. αSyn aggregation disrupts critical cellular processes, including synaptic function, mitochondrial integrity, and proteostasis, which culminate in neuronal cell death. Importantly, αSyn pathology extends beyond neurons—it also encompasses spreading throughout the neuronal environment and internalization by microglia and astrocytes. Once internalized, glia can act as neuroprotective scavengers, which limit the spread of αSyn. However, they can also become reactive, thereby contributing to neuroinflammation and the progression of PD. Recent advances in αSyn research have enabled the molecular diagnosis of PD and accelerated the development of targeted therapies. Nevertheless, despite more than two decades of research, the cellular function, aggregation mechanisms, and induction of cellular damage by αSyn remain incompletely understood. Unraveling the interplay between αSyn, neurons, and glia may provide insights into disease initiation and progression, which may bring us closer to exploring new effective therapeutic strategies. Herein, we provide an overview of recent studies emphasizing the multifaceted nature of αSyn and its impact on both neuron and glial cell damage.
Withaferin A and Celastrol Overwhelm Proteostasis
Authors: Vilaboa Nuria , Voellmy Richard
Published: December 2023

Withaferin A (WA) and celastrol (CEL) are major bioactive components of plants that have been widely employed in traditional medicine. The pleiotropic activities of plant preparations and the isolated compounds in vitro and in vivo have been documented in hundreds of studies. Both WA and CEL were shown to have anticancer activity. Although WA and CEL belong to different chemical classes, our synthesis of the available information suggests that the compounds share basic mechanisms of action. Both WA and CEL bind covalently to numerous proteins, causing the partial unfolding of some of these proteins and of many bystander proteins. The resulting proteotoxic stress, when excessive, leads to cell death. Both WA and CEL trigger the activation of the unfolded protein response (UPR) which, if the proteotoxic stress persists, results in apoptosis mediated by the PERK/eIF-2/ATF4/CHOP pathway or another UPR-dependent pathway. Other mechanisms of cell death may play contributory or even dominant roles depending on cell type. As shown in a proteomic study with WA, the compounds appear to function largely as electrophilic reactants, indiscriminately modifying reachable nucleophilic amino acid side chains of proteins. However, a remarkable degree of target specificity is imparted by the cellular context.
Modulation of TRPV1 and TRPA1 Channels Function by Sea Anemones’ Peptides Enhances the Viability of SH-SY5Y Cell Model of Parkinson’s Disease
Authors: Kolesova Yuliya S. , Stroylova Yulia Y. , Maleeva Ekaterina E. , Moysenovich Anastasia M. , Pozdyshev Denis V. , Muronetz Vladimir I. , Andreev Yaroslav A.
Published: December 2023

Cellular dysfunction during Parkinson’s disease leads to neuroinflammation in various brain regions, inducing neuronal death and contributing to the progression of the disease. Different ion channels may influence the process of neurodegeneration. The peptides Ms 9a-1 and APHC3 can modulate the function of TRPA1 and TRPV1 channels, and we evaluated their cytoprotective effects in differentiated to dopaminergic neuron-like SH-SY5Y cells. We used the stable neuroblastoma cell lines SH-SY5Y, producing wild-type alpha-synuclein and its mutant A53T, which are prone to accumulation of thioflavin-S-positive aggregates. We analyzed the viability of cells, as well as the mRNA expression levels of TRPA1, TRPV1, ASIC1a channels, alpha-synuclein, and tyrosine hydroxylase after differentiation of these cell lines using RT-PCR. Overexpression of alpha-synuclein showed a neuroprotective effect and was accompanied by a reduction of tyrosine hydroxylase expression. A mutant alpha-synuclein A53T significantly increased the expression of the pro-apoptotic protein BAX and made cells more susceptible to apoptosis. Generally, overexpression of alpha-synuclein could be a model for the early stages of PD, while expression of mutant alpha-synuclein A53T mimics a genetic variant of PD. The peptides Ms 9a-1 and APHC3 significantly reduced the susceptibility to apoptosis of all cell lines but differentially influenced the expression of the genes of interest. Therefore, these modulators of TRPA1 and TRPV1 have the potential for the development of new therapeutic agents for neurodegenerative disease treatment.
Involvement of PAR-2 in the Induction of Cell-Specific Matrix Metalloproteinase-2 by Activated Protein C in Cutaneous Wound Healing
Authors: Julovi Sohel M. , McKelvey Kelly , Minhas Nikita , Chan Yee-Ka Agnes , Xue Meilang , Jackson Christopher J.
Published: December 2023

We previously reported that human keratinocytes express protease-activated receptor (PAR)-2 and play an important role in activated protein C (APC)-induced cutaneous wound healing. This study investigated the involvement of PAR-2 in the production of gelatinolytic matrix metalloproteinases (MMP)-2 and -9 by APC during cutaneous wound healing. Full-thickness excisional wounds were made on the dorsum of male C57BL/6 mice. Wounds were treated with APC on days 1, 2, and 3 post-wounding. Cultured neonatal foreskin keratinocytes were treated with APC with or without intact PAR-2 signalling to examine the effects on MMP-2 and MMP-9 production. Murine dermal fibroblasts from PAR-2 knock-out (KO) mice were also assessed. MMP-2 and -9 were measured via gelatin zymography, fluorometric assay, and immunohistochemistry. APC accelerated wound healing in WT mice, but had a negligible effect in PAR-2 KO mice. APC-stimulated murine cutaneous wound healing was associated with the differential and temporal production of MMP-2 and MMP-9, with the latter peaking on day 1 and the former on day 6. Inhibition of PAR-2 in human keratinocytes reduced APC-induced MMP-2 activity by 25~50%, but had little effect on MMP-9. Similarly, APC-induced MMP-2 activation was reduced by 40% in cultured dermal fibroblasts derived from PAR-2 KO mice. This study shows for the first time that PAR-2 is essential for APC-induced MMP-2 production. Considering the important role of MMP-2 in wound healing, this work helps explain the underlying mechanisms of action of APC to promote wound healing through PAR-2.
Peptidomics Unveils Distinct Acetylation Patterns of Histone and Annexin A1 in Differentiated Thyroid Cancer
Authors: Coelho Margarida , Capela João , Mendes Vera M. , Pacheco João , Fernandes Margarida Sá , Amendoeira Isabel , Jones John G. , Raposo Luís , Manadas Bruno
Published: December 2023

Thyroid cancer is a common malignancy of the endocrine system. Nodules are routinely evaluated for malignancy risk by fine needle aspiration biopsy (FNAB), and in cases such as follicular lesions, differential diagnosis between benign and malignant nodules is highly uncertain. Therefore, the discovery of new biomarkers for this disease could be helpful in improving diagnostic accuracy. Thyroid nodule biopsies were subjected to a precipitation step with both the insoluble and supernatant fractions subjected to proteome and peptidome profiling. Proteomic analysis identified annexin A1 as a potential biomarker of thyroid cancer malignancy, with its levels increased in malignant samples. Also upregulated were the acetylated peptides of annexin A1, revealed by the peptidome analysis of the supernatant fraction. In addition, supernatant peptidomic analysis revealed a number of acetylated histone peptides that were significantly elevated in the malignant group, suggesting higher gene transcription activity in malignant tissue. Two of these peptides were found to be robust malignancy predictors, with an area under the receiver operating a characteristic curve (ROC AUC) above 0.95. Thus, this combination of proteomics and peptidomics analyses improved the detection of malignant lesions and also provided new evidence linking thyroid cancer development to heightened transcription activity. This study demonstrates the importance of peptidomic profiling in complementing traditional proteomics approaches.
GmCBP60b Plays Both Positive and Negative Roles in Plant Immunity
Authors: Ye Mei-Yan , Lan Hu-Jiao , Liu Jian-Zhong
Published: December 2023

CBP60b (CALMODULIN-BINDING PROTEIN 60b) is a member of the CBP60 transcription factor family. In Arabidopsis, AtCBP60b not only regulates growth and development but also activates the transcriptions in immune responses. So far, CBP60b has only been studied extensively in the model plant Arabidopsis and rarely in crops. In this study, Bean pod mottle virus (BPMV)-mediated gene silencing (BPMV-VIGS) was used to silence GmCBP60b.1/2 in soybean plants. The silencing of GmCBP60b.1/2 resulted in typical autoimmunity, such as dwarfism and enhanced resistance to both Soybean mosaic virus (SMV) and Pseudomonas syringae pv. glycinea (Psg). To further understand the roles of GmCBP60b in immunity and circumvent the recalcitrance of soybean transformation, we generated transgenic tobacco lines that overexpress GmCBP60b.1. The overexpression of GmCBP60b.1 also resulted in autoimmunity, including spontaneous cell death on the leaves, highly induced expression of PATHOGENESIS-RELATED (PR) genes, significantly elevated accumulation of defense hormone salicylic acid (SA), and significantly enhanced resistance to Pst DC3000 (Pseudomonas syrangae pv. tomato DC3000). The transient coexpression of a luciferase reporter gene driven by the promoter of soybean SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (GmSARD1) (ProGmSARD1::LUC), together with GmCBP60b.1 driven by the 35S promoter, led to the activation of the LUC reporter gene, suggesting that GmCBP60b.1 could bind to the core (A/T)AATT motifs within the promoter region of GmSARD1 and, thus, activate the expression of the LUC reporter. Taken together, our results indicate that GmCBP60b.1/2 play both positive and negative regulatory roles in immune responses. These results also suggest that the function of CBP60b is conserved across plant species.
Chemokines in Cartilage Regeneration and Degradation: New Insights
Authors: Edderkaoui Bouchra
Published: December 2023

Cartilage plays a crucial role in the human body by forming long bones during development and growth to bear loads on joints and intervertebral discs. However, the increasing prevalence of cartilage degenerative disorders is a growing public health concern, especially due to the poor innate regenerative capacity of cartilage. Chondrocytes are a source of several inflammatory mediators that play vital roles in the pathogenesis of cartilage disorders. Among these mediators, chemokines have been explored as potential contributors to cartilage degeneration and regeneration. Our review focuses on the progress made during the last ten years in identifying the regulators and roles of chemokines and their receptors in different mechanisms related to chondrocytes and cartilage. Recent findings have demonstrated that chemokines influence cartilage both positively and negatively. Their induction and involvement in either process depends on the local molecular environment and is both site- and time-dependent. One of the challenges in defining the role of chemokines in cartilage pathology or regeneration is the apparent redundancy in the interaction of chemokines with their receptors. Hence, it is crucial to determine, for each situation, whether targeting specific chemokines or their receptors will help in developing effective therapeutic strategies for cartilage repair.
Predictive Factors for Successful Cervical Ripening among Women with Gestational Diabetes Mellitus at Term: A Prospective Study
Authors: Ducarme Guillaume , Planche Lucie , Lbakhar Mounia
Published: December 2023

The purpose of this prospective cohort study is to identify the predictive factors for vaginal delivery among women (n = 146) who underwent cervical ripening using a dinoprostone insert (PG) alone (13.7%), cervical ripening balloon (CRB) alone (52.7%), oral misoprostol (M) alone (4.1%), or repeated methods (R, 29.5%) for gestational diabetes mellitus (GDM) at term, and to analyze maternal and neonatal morbidity outcomes according to the method for cervical ripening. After cervical ripening, vaginal delivery occurred in 84.2% (n = 123) and was similar among groups (90.0% after PG, 83.1% after CRB, 83.3% after M, and 83.7% after R; p = 0.89). After a multivariable logistic regression analysis adjusted for potential confounders, the internal cervical os being open before cervical ripening was a predictor of vaginal delivery (adjusted odds ratio (OR) of 4.38, 95% confidence index (CI) of 1.62–13.3, p = 0.03), and previous cesarean delivery was a predictor of cesarean delivery (aOR of 7.67, 95% CI of 2.49–24.00, p < 0.01). Birthweight was also significantly associated with cesarean delivery (aOR of 1.15, 95% CI of 1.03–1.31, p = 0.02). The rates of maternal and neonatal morbidity outcomes were 10.9% (n = 16) and 19.9% (n = 29), respectively, and did not differ according to the mode of delivery and to the method used for cervical ripening. Identifying these specific high-risk women (previous cesarean delivery and internal cervical os being closed before cervical ripening) for cesarean delivery among women who underwent cervical ripening for GDM at term is important and practical for all physicians to make a decision in partnership with women.
Safety of Duodenal Endoscopic Submucosal Dissection for Superficial Non-Ampullary Duodenal Epithelial Tumor: A Single-Center Study in the United States
Authors: Koseki Mako , Nishimura Makoto , Nammour Tarek , Chin Kana , Nagao Sayaka , Beauvais Jacques C. , Schattner Mark A.
Published: December 2023

Endoscopic submucosal dissection (ESD) of superficial non—ampullary duodenal epithelial tumors (SNADETs) is associated with a high rate of en bloc resection and low rate of recurrence. However, in the United States, SNADETs are predominantly managed using endoscopic mucosal resection (EMR) or surgery because the feasibility and safety of duodenal ESD have not yet been established. In this study, we analyzed the outcomes of duodenal ESD for SNADETs. This single—center retrospective study reviewed the data of patients who underwent ESD for SNADETs between June 2018 and August 2023. Baseline patient characteristics, histopathology of the resected lesions, adverse events, and recurrence rates were evaluated. The primary outcome measures were en bloc resection, complications, and recurrence rate. Thirty ESD procedures were performed on 24 patients. All 30 lesions were adenomas, with no cancerous lesions. The en bloc resection rate and R0 resection rates were both 53%. There were no cases of procedure-associated perforation. Post-ESD bleeding was observed in six cases. No ESD—related mortality was observed. The recurrence rate was 14% in 1 year follow up, and 28% the during all follow-up period. ESD is a safe option for SNADET in the United States; however further comparative studies are necessary to determine the optimal procedure for North American populations.
Obstructive Sleep Apnea and Its Influence on Intracranial Aneurysm
Authors: Jung Tae Young , Lee Eunkyu , Park Minhae , Lee Jin-Young , Hong Yun Soo , Cho Juhee , Guallar Eliseo , Hong Sang Duk , Jung Yong Gi , Gu Seonhye , Ryoo Jae Wook , Joo Eun-Yeon , Yeon Je Young , Ryu Gwanghui , Kim Hyo Yeol
Published: December 2023

Obstructive sleep apnea syndrome (OSAS) is associated with cerebrovascular disease, which can lead to life-threatening outcomes. The purpose of the study was to investigate the relationship between OSAS and comorbid intracranial aneurysms. We retrospectively reviewed 564 patients who underwent a polysomnography and brain magnetic resonance angiography as part of their health checkup. We calculated the prevalence of an intracranial aneurysm and OSAS in patients and measured the size of the intracranial aneurysm if present. The mean patient age was 55.6 ± 8.5 years, and 82.3% of them were men. The prevalence of an intracranial aneurysm in patients with OSAS was 12.1%, which is significantly higher than patients with non-OSAS (5.9%, p = 0.031). Patients with OSAS had a much higher prevalence of intracranial aneurysms, after adjusting all possible confounding factors such as age, sex, smoking status, alcohol drinking, and body mass index (odds ratio: 2.32; 95% confidence interval: 1.07–5.04). Additionally, the OSAS group had noticeably larger aneurysms compared with those of the non-OSAS group (3.2 ± 2.0 mm vs. 2.0 ± 0.4 mm, p = 0.013). We found a significant association between OSAS and intracranial aneurysms. OSAS could be another risk factor for the development of intracranial aneurysms.
Effects of Immersive Virtual Reality on Upper-Extremity Stroke Rehabilitation: A Systematic Review with Meta-Analysis
Authors: Kiper Pawel , Godart Nathalie , Cavalier Manon , Berard Charlotte , Cieślik Błażej , Federico Sara , Kiper Aleksandra , Pellicciari Leonardo , Meroni Roberto
Published: December 2023

Virtual reality (VR) is an innovative rehabilitation tool increasingly used in stroke rehabilitation. Fully immersive VR is a type of VR that closely simulates real-life scenarios, providing a high level of immersion, and has shown promising results in improving rehabilitation functions. This study aimed to assess the effect of immersive VR-based therapy for stroke patients on the upper extremities, activities of daily living (ADLs), and pain reduction and its acceptability and side effects. For this review, we gathered all suitable randomized controlled trials from PubMed, EMBASE, Cochrane Library, Scopus, and Web of Science. Out of 1532, 10 articles were included, with 324 participants. The results show that immersive VR offers greater benefits in comparison with conventional rehabilitation, with significant improvements observed in ADLs (SMD 0.58, 95% CI 0.25 to 0.91, I2 = 0%, p = 0.0005), overall function as measured by the Fugl-Meyer Assessment (MD 6.33, 95% CI 4.15 to 8.50, I2 = 25%, p = 0.00001), and subscales for the shoulder (MD 4.96, 95% CI—1.90–8.03, I2 = 25%, p = 0.002), wrist (MD 2.41, 95% CI—0.56–4.26, I2 = 0%, p = 0.01), and hand (MD 2.60, 95% CI—0.70–4.5°, I2 = 0%, p = 0.007). These findings highlight the potential of immersive VR as a valuable therapeutic option for stroke survivors, enhancing their ADL performance and upper-limb function. The immersive nature of VR provides an engaging and immersive environment for rehabilitation.
Myocarditis and Chronic Inflammatory Cardiomyopathy, from Acute Inflammation to Chronic Inflammatory Damage: An Update on Pathophysiology and Diagnosis
Authors: Uccello Giuseppe , Bonacchi Giacomo , Rossi Valentina Alice , Montrasio Giulia , Beltrami Matteo
Published: December 2023

Acute myocarditis covers a wide spectrum of clinical presentations, from uncomplicated myocarditis to severe forms complicated by hemodynamic instability and ventricular arrhythmias; however, all these forms are characterized by acute myocardial inflammation. The term “chronic inflammatory cardiomyopathy” describes a persistent/chronic inflammatory condition with a clinical phenotype of dilated and/or hypokinetic cardiomyopathy associated with symptoms of heart failure and increased risk for arrhythmias. A continuum can be identified between these two conditions. The importance of early diagnosis has grown markedly in the contemporary era with various diagnostic tools available. While cardiac magnetic resonance (CMR) is valid for diagnosis and follow-up, endomyocardial biopsy (EMB) should be considered as a first-line diagnostic modality in all unexplained acute cardiomyopathies complicated by hemodynamic instability and ventricular arrhythmias, considering the local expertise. Genetic counseling should be recommended in those cases where a genotype–phenotype association is suspected, as this has significant implications for patients’ and their family members’ prognoses. Recognition of the pathophysiological pathway and clinical “red flags” and an early diagnosis may help us understand mechanisms of progression, tailor long-term preventive and therapeutic strategies for this complex disease, and ultimately improve clinical outcomes.
Gastric Outlet Obstruction from Stomach-Containing Groin Hernias: Case Report and a Systematic Review
Authors: Favela Juan G. , Argo Madison B. , McAllister Jared , Waldrop Caitlyn L. , Huerta Sergio
Published: December 2023

Most abdominopelvic structures can find their way to a groin hernia. However, location, and relative fixation are important for migration. Gastric outlet obstruction (GOO) from a stomach-containing groin hernia (SCOGH) is exceedingly rare. In the current report, we present a 77-year-old man who presented with GOO from SCOGH to our facility. We performed a review of the literature following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) of patients presenting with SCOGH since it was first reported in 1802. Ninety-one cases of SCOGH were identified (85 inguinal and six femoral) over the last two centuries (1802–2023). GOO from SCOGH occurred in 48% of patients in one review and 18% in our systematic analysis. Initial presentation ranged from a completely asymptomatic patient to peritonitis. Management varied from entirely conservative treatment to elective hernia repair to emergent laparotomy. Only one case of laparoscopic management was documented. Twenty-one deaths from SCOGH were reported, with most occurring in early manuscripts (1802–1896 [n = 9] and 1910–1997 [n = 10]). In the recent medical era, outcomes for patients with this rare clinical presentation are satisfactory and treatment ranging from conservative, non-operative management to surgical repair should be tailored towards patients’ clinical presentation.
Management and Treatment for Dysphagia in Neurodegenerative Disorders
Authors: Ueha Rumi , Cotaoco Carmel , Kondo Kenji , Yamasoba Tatsuya
Published: December 2023

Patients with neurodegenerative disorders (NDDs) often experience functional dysphagia, which may involve dysfunction in a specific phase of swallowing or in the entire process. This review outlines the approach to dysphagia in the setting of NDDs. Distinguishing the etiology of dysphagia can be difficult, and it is important to always look out for signs pointing to NDD as the cause. Thorough diagnostic work-up is essential, and it includes a comprehensive history and physical examination, alongside swallowing function tests, such as fiberoptic endoscopic evaluation of swallowing, videofluoroscopic swallowing study, and high-resolution manometry. Management requires a multidisciplinary approach with a treatment plan tailored to each patient. This involves dietary guidance, swallowing rehabilitation, and surgery in cases in which improvement with rehabilitation is inadequate. Surgery may involve altering certain pharyngolaryngeal structures to facilitate swallowing and reduce the risk of aspiration (swallowing improvement surgery) or separating the airway and digestive tract while sacrificing laryngeal function, with the main goal of preventing aspiration (aspiration prevention surgery). Proper management stems from recognizing the impact of these disorders on swallowing and consistently finding ways to improve the quality of life of patients.
Emerging Perspectives on the Set of Conditions That Lead to the Emergence of Metabolic Syndrome
Authors: Tarcău Bogdan M. , Vicaș Laura G. , Filip Lorena , Maghiar Florin , Șandor Mircea , Pallag Annamaria , Jurca Tunde , Mureșan Mariana Eugenia , Marian Eleonora
Published: December 2023

Metabolic syndrome, as a medical condition, presents multifactorial complexity that is characterized by the resulting damage from genetic, environmental, and lifestyle factors (presence or absence of physical activity, food choices). Thus, metabolic syndrome qualifies unequivocally as a medical condition in which there are, simultaneously, several independent metabolic risk factors, namely, abdominal obesity, high triglyceride level, low HDL cholesterol level, arterial hypertension, and high glycemic level. Although age, sex, socio-economic status, and the precise definition of metabolic syndrome all influence the prevalence and risk of developing the condition, clinical and epidemiological studies clearly show that central obesity, as measured by an increased abdominal circumference, is the main risk factor. Thus, due to the growing global incidence of obesity, there has been an increase in the incidence of metabolic syndrome. Starting with obesity, all other metabolic risk factors are influenced: for example, as a result of insulin resistance with hyperglycemia, diabetes is linked to an increased risk of cardiovascular disease due to increased abdominal circumference. Through this review, we aimed to highlight the latest research studies and dietary nutritional interventions useful in the prevention of this disease but also implementation strategies for primary prevention among the healthy population.
Application of New Al-Si Welding Filler with High Concentration of Copper and Magnesium: High-Temperature Strength and Anti-Corrosion Mechanism
Authors: Zhao Jun-Ren , Hung Fei-Yi , Pan Chien-Yu
Published: December 2023

Currently, the primary commercial aluminum alloy fillers used are 4043 and 5356. However, when welded with high-strength work pieces like 6061 and 7075 aluminum alloys, the strength of weld beads significantly lags behind that of the original welded material. Both 4043 and 5356 aluminum alloys cannot be strengthened through heat treatment. The strength difference between the weld bead and base material doubles after heat treatment. In this study, an Al-Si-Cu-Mg alloy (SCM) filler modified using a heat-treatable A319 aluminum alloy was employed to investigate the post-welding microstructural and mechanical properties of specimens under room- and high-temperature conditions and after prolonged exposure in a saltwater environment (3.5 wt.% NaCl). The aim was to demonstrate that commercial aluminum alloy fillers could be substituted with a high-silicon aluminum alloy boasting excellent solidification and mechanical properties. The results revealed that, after heat treatment of the weld bead, dendrites were not eliminated, but the tensile strength increased to 310 MPa, closely matching that of commercial 6061 aluminum alloy. The strength of the weld bead remained higher than 250 MPa in high-temperature (240 °C) and saltwater environments. These findings underscore the potential application of this material.
Advances in the Experimentation and Numerical Modeling of Material Joining Processes
Authors: Campilho Raul D. S. G.
Published: December 2023

Material joining processes are a critical factor in engineering structures since they influence such structures’ structural integrity, performance, and longevity [...]