bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation of hydroxyapatite nanofibers by using ionic liquids as template and application in enhancing hydrogel performance
Authors: Ren Xiuli , Liang Zepeng , Zhao Xingjun
Published: August 2023

Introduction: Hydroxyapatite (HAP or HA) nanofibers are very attractive in the field of biomedical engineering. However, templates used for preparing HAP nanofibers are usually hydrophobic molecules, like fatty acids and/or surfactants, which are difficult to remove and potentially toxic. Therefore, it is important to develop a green approach to prepare HAP nanofibers.

Methods: Imidazolium-based ionic liquids (ILs) were used as templates to control the crystallization of HAP. The obtained HAP nanofibers were composited into polyvinyl alcohol-sodium alginate (PVA-Alg) hydrogel (HAP@H). The rheological performance, stretching, and compression properties were tested. Scanning electron microscope (SEM), high resolution transmission electron microscope (HRTEM), X-ray diffraction (XRD), Fourier-transform infrared (FT-IR), and differential scanning calorimetry (DSC) were adopted to characterize the morphology, size, crystallographic orientations, and phase of HAP@H.

Results: HAP nanofibers with a length of ∼50 μm were harvested. The DSC results proved that water loss temperature increased from 98°C (for pure hydrogel) to 107°C (for HAP@H). Also, HAP@H hydrogel presented much better porous structure, tensile performance, and compressive performance than that of pure hydrogel.

Discussion: The morphology, size, and growth direction of HAP could be modulated easily by altering the alkyl chain length of ILs’ cations. This is possibly due to face-specific adsorption of imidazolium moieties on HAP nanocrystals. The enhancing performance of HAP@H is probably due to the composited highly oriented HAP nanofibers.
Dissecting the molecular trajectory of fibroblast reprogramming to chemically induced mammary epithelial cells
Authors: Qin Liangshan , Zhang Dandan , Liu Siyi , Liu Quanhui , Liu Mingxing , Huang Ben
Published: August 2023

Introduction: The plasticity of cell identity allows cellular reprogramming that manipulates the lineage of cells to generate the target cell types, bringing new avenues for disease modeling and autologous tailored cell therapy. Previously, we had already successfully established a technical platform for inducing fibroblast reprogramming to chemically induced mammary epithelial cells (CiMECs) by small-molecule compounds. However, exactly how the molecular mechanism driving the lineage conversion remains unknown.

Methods: We employ the RNA-sequencing technology to investigate the transcriptome event during the reprogramming process and reveal the molecular mechanisms for the fate acquisition of mammary lineage.

Results: The multi-step reprogramming process first overcomes multiple barriers, including the inhibition of mesenchymal characteristics, pro-inflammatory and cell death signals, and then enters an intermediate plastic state. Subsequently, the hormone and mammary development genes were rapidly activated, leading to the acquisition of the mammary program together with upregulation of the milk protein synthesis signal. Moreover, the gene network analyses reveal the potential relationship between the TGF-β signaling pathway to mammary lineage activation, and the changes in the expression of these genes may play important roles in coordinating the reprogramming process.

Conclusion: Together, these findings provide critical insights into the molecular route and mechanism triggered by small-molecule compounds that induce fibroblast reprogramming into the fate of mammary epithelial cells, and they also laid a foundation for the subsequent research on the development and differentiation of mammary epithelial cells and lactation.
Mid-term changes in cognitive functions in patients with atrial fibrillation: a longitudinal analysis of the Swiss-AF cohort
Authors: Wueest Alexandra S. , Zuber Priska , Coslovsky Michael , Rommers Nikki , Rodondi Nicolas , Gencer Baris , Moschovitis Giorgio , De Perna Maria Luisa , Beer Juerg H. , Reichlin Tobias , Krisai Philipp , Springer Anne , Conen David , Stauber Annina , Mueller Andreas S. , Paladini Rebecca E. , Kuhne Michael , Osswald Stefan , Monsch Andreas U. , Bonati Leo H.
Published: August 2023

Background

Longitudinal association studies of atrial fibrillation (AF) and cognitive functions have shown an unclear role of AF-type and often differ in methodological aspects. We therefore aim to investigate longitudinal changes in cognitive functions in association with AF-type (non-paroxysmal vs. paroxysmal) and comorbidities in the Swiss-AF cohort.

Methods

Seven cognitive measures were administered up to five times between 2014 and 2022. Age-education standardized scores were calculated and association between longitudinal change in scores and baseline AF-type investigated using linear mixed-effects models. Associations between AF-type and time to cognitive drop, an observed score of at least one standard deviation below individual's age-education standardized cognitive scores at baseline, were studied using Cox proportional hazard models of each cognitive test, censoring patients at their last measurement. Models were adjusted for baseline covariates.

Results

2,415 AF patients (mean age 73.2 years; 1,080 paroxysmal, 1,335 non-paroxysmal AF) participated in this Swiss multicenter prospective cohort study. Mean cognitive scores increased longitudinally (median follow-up 3.97 years). Non-paroxysmal AF patients showed smaller longitudinal increases in Digit Symbol Substitution Test (DSST), Cognitive Construct Score (CoCo)and Trail Making Test part B (TMT-B) scores vs. paroxysmal AF patients. Diabetes, history of stroke/TIA and depression were associated with worse performance on all cognitive tests. No differences in time to cognitive drop were observed between AF-types in any cognitive test.

Conclusion

This study indicated preserved cognitive functioning in AF patients, best explained by practice effects. Smaller practice effects were found in non-paroxysmal AF patients in the DSST, TMT-B and the CoCo and could indicate a marker of subtle cognitive decline. As diabetes, history of stroke/TIA and depression—but not AF-type—were associated with cognitive drop, more attention should be given to risk factors and underlying mechanisms of AF.
Mechanics of the Kalabagh Fault, northwest Himalayan fold and thrust belt (convergence zone of India and Eurasia), using SAR interferometry and CFS
Authors: Zafar Waqar Ali , Javed Farhan , Ahmed Rizwan , Ehsan Muhsan , Abdelrahman Kamal , Fnais Mohammed S. , Qureshi Mansoor Aziz
Published: August 2023

The Kalabagh strike–slip fault, which is characterized by right-lateral movement, is part of the northwestern Himalayan foreland fold and thrust belt in Pakistan. This structure marks the western and eastern terminations of the Salt Range and Surghar Ranges, respectively. No significant (>M6) earthquakes have been reported along the Kalabagh Fault in recent decades. Here, we take advantage of space-borne Sentinel-1A SAR interferometry to gain insight into the mechanics of faulting, aseismic creeping, and stress loading of the seismic cycle on the Kalabagh Fault spanning over approximately 7 years. In this study, we also removed the tropospheric effects using the Generic Atmospheric Correction Online Service data from the rate map. We further resolved the LOS deformation into both horizontal and vertical deformations. Our Bayesian inversion indicates that the fault experiences significant horizontal and vertical displacements. The fault’s southern and northern segments exhibit a creeping rate of approximately ∼4.2 ± 1.3 to 4.8 ± 1.6 mm/year, respectively, while the central section does not display any horizontal creeping. We found that the creeping is confined between 0 and ∼2.7 ± 1.1 km depth at the northern section and 0 and ∼3.9 ± 1.1 km on the southern section of the faults. Nevertheless, we found that the vertical creeping of ∼10 mm/year is confined between 0.5 and 6 km depth in the central segment of the fault. Moreover, our model does not resolve the interseismic slip at depth on the Kalabagh Fault. Our results affirm that Kalabagh Fault is creeping, and the internal deformation due to the presence of a thick salt layer over the decollement facilitates the creeping on this fault. In addition, Coulomb stress modeling depicts that the creeping on the Kalabagh Fault increases the Coulomb stress changes in the northern section of the KBF.
Most postoperative reserved “normal” metatarsal stumps of diabetic foot osteomyelitis are infected but have healing potential
Authors: Xu Jun , Chen Weiling , He Lu , Feng Shuhong , Zhang Jinghang , Chang Bai
Published: August 2023

Background

Although the pathology and bacterial status of the “normal” bone stump after operation of diabetic foot osteomyelitis (DFO) are of great significance for the prognosis of foot wounds, there are only a few studies on this topic; hence, it is clinically relevant and urgent to study this topic.

Methods

The data of 57 inpatients with DFO from June 2021 to April 2022 were collected, all of whom had DFO in the forefoot and underwent conservative surgery. After the surgical removal of necrotic bone, bone biopsies were taken from the necrotic phalangeal bone and the reserved “normal” metatarsal stump. They were cultured, after which antibiotic susceptibility test and pathological screening were carried out. According to clinical judgment, inpatients’ wounds were divided into metatarsal affected group and metatarsal unaffected group. We then compared and analyzed the pathological and bacterial characteristics of preserved “normal” bone stump and its effect on wound healing and prognosis.

Results

The poor concordance rate between deep soft tissue culture and infected phalange culture was only 19.3%. The deep soft tissue (72.6%), infected phalange (70.7%), and metatarsal stump (71.4%) were mainly infected with gram-negative Bacillus. The proportion of Enterococcus spp. increased significantly in bone tissue. Acinetobacter baumannii had the highest drug resistance (88%, 22/25). There was no significant difference in several clinical characteristics and wound healing regardless of whether their metatarsal stumps were affected. Most reserved “normal” metatarsal stumps (84.2%, 48/57) were positive by pathological diagnosis and bacterial culture testing; only 15.7% (9/57) samples were truly sterile. Only 8.3% (4/48) of the former patients healed within 6 months; whereas, all the latter (9/9) patients healed within 6 months. However, the majority (89.6%, 43/48) could heal. There was no difference in operations, skin grafting, negative pressure wound therapy, and mortality between the two groups.

Conclusion

The most reserved “normal” metatarsal stumps have been invaded by bacteria. However, the majority stumps can be preserved, and the wound will eventually be healed according to the pathological and bacterial culture results.
Predicting air quality using a quantitative forecasting model of PM2.5 micro-scale variation nested with wave spectrum analysis
Authors: Wang Deying , Wang Jizhi , Yang Yuanqin , Jia Wenxing , Zhong Junting , Wang Yaqiang , Zhang Xiaoye
Published: August 2023

This study focused on weather and environmental numerical prediction and public demand. It expanded the concept and technology growth points in new fields in terms of new tasks for major prediction services for “large-scale public events.” This is required for more advanced prediction and to improve the resolution, fineness, and accuracy of the prediction. This study explored the prediction theory and technical application of transient atmospheric aerosol pollution within an accuracy of an hour. The novelty of this study is as follows: ①Based on high-quality big data covering the Northern Hemisphere with high temporal resolution with an accuracy of 1 h, a quantitative theory of the “natural weather cycle” spectral analysis algorithm was developed. This study presented a quantitative forecast model that nests the “spectral analysis of atmospheric wave-like disturbance” in the westerly belt with the “transient characteristics” of micro-scale aerosols (PM2.5 concentration) in Beijing and North China. ②According to the nested model of this study, the wave-like oscillation (H′) of 500 hPa was positively correlated with the PLAM index and PM2.5 mass concentration during nested multi-“natural weather cycles.” The significance level exceeded 0.001. This study demonstrated the prediction abilities of early quantitative fine prediction theory and implementation in the context of air quality. The forecast service on 1 October 2022, for the opening of the CCP 20th National Congress (16 October), and during the conference was successfully presented in real time. The results of this study on hourly resolution high-precision air quality forecasting service showed that rolling forecasts can be continuously released both 1 month and 7–10 days in advance, and the nesting effect can constantly be updated. Forecasts were found to be consistent with reality. ③The nested mode method for atmospheric spectrum analysis and micro-scale aerosol (PM2.5) distribution provides quantitative analysis and a decision-making basis for business-oriented operations to address technical difficulties.
Impact of economic growth on the changes in forest resources in Inner Mongolia of China
Authors: Wu Xiaoyu , Liu Guiyan , Bao Qingfeng
Published: August 2023

Balancing the relationship between forest resources and economic growth is crucial for achieving sustainable development in China. Inner Mongolia, with its unique geographical environment and relatively underdeveloped economy, exhibits distinctive patterns in its forest resources development and changes. This study investigates the impact of economic growth on forest resources in Inner Mongolia using time-series data from 1980 to 2018 and the cubic curve regression model based on the Environmental Kuznets Curve (EKC) hypothesis. The results reveal an inverted N-shaped EKC relationship, with the forest coverage rate undergoing a “decline-up-decline” evolution process, characterized by turning points 1984 and 2015, and the forest stock volume exhibiting a “decline-up” evolution process with a minimum turning point around 1999. Remarkably, the forest stock volume continues to increase at present and over a foreseeable future. Furthermore, the study finds that population density has a negligible effect on the reduction of forest coverage rate in Inner Mongolia. Lastly, the paper offers policy suggestions to promote the sustainable development of forest resources in the region.
The implication of necroptosis-related lncRNAs in orchestrating immune infiltration and predicting therapeutic efficacy in colon adenocarcinoma: an integrated bioinformatic analysis with preliminarily experimental validation
Authors: Li Shizhe , Wang Xiaotong , Liu Yajun , Xiao Junbo , Yi Jun
Published: August 2023

Background: Necroptosis contributes significantly to colon adenocarcinoma (COAD). We aim to assess the relationship between immunoinfiltration and stemness in COAD patients through the development of a risk score profile using necroptosis-related long noncoding RNAs (NRLs).

Methods: Our study was based on gene expression data and relevant clinical information from The Cancer Genome Atlas (TCGA). Necroptosis-related genes (NRGs) were obtained from the Kyoto Encyclopedia of Genes and Genome (KEGG) database. Pearson correlation analysis, Cox regression, and least absolute shrinkage and selection operator (LASSO) regression were used to determine the NRL prognositic signature (NRLPS). NRLs expression was examined using qRT-PCR method. Several algorithms were used to identify relationships between immune cell infiltration and NRLPS risk scores. Further analysis of somatic mutations, tumor stemness index (TSI), and drug sensitivity were also explored.

Results: To construct NRLPS, 15 lncRNAs were investigated. Furthermore, NRLPS patients with high-risk subgroups had lower survival rates than that of patients with low-risk subgroups. Using GSEA analysis, NRL was found to be enriched in Notch, Hedgehog and Smoothened pathways. Immune infiltration analysis showed significant differences in CD8+ T cells, dendritic cell DCs, and CD4+ T cells between the two risk groups. In addition, our NRLPS showed a relevance with the regulation of tumor microenvironment, tumor mutation burden (TMB) and stemness. Finally, NRLPS demonstrated potential applications in predicting the efficacy of immunotherapy and chemotherapy in patients with COAD.

Conclusion: Based on NRLs, a prognostic model was developed for COAD patients that allows a personalized tailoring immunotherapy and chemotherapy to be tailored.
CHDH, a key mitochondrial enzyme, plays a diagnostic role in metabolic disorders diseases and tumor progression
Authors: Li Yifei , Shen Xinzhuang , Yang Xiaowen , Lian Fuming , Li Yanping , Li Jinmeng , Huang Yongming , Shen Wenzhi , Liu Huan
Published: August 2023

Human choline dehydrogenase (CHDH) is a transmembrane protein located in mitochondria. CHDH has been shown to be one of the important catalytic enzymes that catalyze the oxidation of choline to betaine and is involved in mitochondrial autophagy after mitochondrial damage. In recent years, an increasing number of studies have focused on CHDH and found a close association with the pathogenesis of various diseases, including tumor prognosis. Here we summarized the genomic localization, protein structure and basic functions of CHDH and discuss the progress of CHDH research in metabolic disorders and other diseases. Moreover, we described the regulatory role of CHDH on the progression of different types of malignant tumors. In addition, major pathogenic mechanisms of CHDH in multiple diseases may be associated with single nucleotide polymorphism (SNP). We look forward to providing new strategies and basis for clinical diagnosis and prognosis prediction of diseases by diagnosing SNP loci of CHDH genes. Our work evaluates the feasibility of CHDH as a molecular marker relevant to the diagnosis of some metabolic disorders diseases and tumors, which may provide new targets for the treatment of related diseases and tumors.
Rheumatoid arthritis and hypothyroidism: a bidirectional Mendelian randomization study
Authors: Duan Lincheng , Chen Dongqing , Shi Yue , Ye Senlin , Dou Shulin , Feng Yue
Published: August 2023

Object

Though significant correlations between rheumatoid arthritis (RA) and hypothyroidism have been found in earlier observational studies, their underlying causal relationship is still unknown. Mendelian randomization (MR) was used in the current study to assess the bidirectional causation between RA and hypothyroidism.

Method

We gathered summary data from genome-wide association studies (GWASs) of RA and hypothyroidism in people of European descent. Then, using data from the FinnGen consortium, we replicated our findings. Three approaches were employed to assess the causal link between RA and hypothyroidism: MR-Egger, weighted median (WM), and inverse variance weighted (IVW). The pleiotropy and heterogeneity were examined using a variety of techniques, including the MR-Egger intercept, the MR-PRESSO approach, the leave-one-out method, and the Cochran’s Q test.

Results

The study looked at a bidirectional incidental relationship between RA and hypothyroidism. The risk of hypothyroidism increased with RA (IVW odds ratio (OR) = 1.28, 95% confidence interval (CI) = 1.18–1.39, P = 8.30E-10), as did the risk of secondary hypothyroidism (IVW OR = 1.12, 95% CI = 1.05–1.21, P = 9.64E-4). The results of reverse MR analysis revealed that hypothyroidism (IVW OR = 1.68, 95% CI = 1.51–1.88, P = 4.87E-21) and secondary hypothyroidism (IVW OR = 1.74, 95% CI = 1.50–2.01, P = 1.91E-13) were linked to an increased risk of RA. Additionally, we obtain the same results in the duplicated datasets as well, which makes our results even more reliable. This study revealed no evidence of horizontal pleiotropy.

Conclusion

The present study established a bidirectional causal link between RA and hypothyroidism. However, it differs slightly from the findings of prior observational studies, suggesting that future research should concentrate on the interaction mechanisms between RA and hypothyroidism.
Extracellular vesicles of Trypanosoma cruzi and immune complexes they form with sialylated and non-sialylated IgGs increase small peritoneal macrophage subpopulation and elicit different cytokines profiles
Authors: Cornet-Gomez Alberto , Moreira Lissette Retana , Gomez-Samblás Mercedes , Osuna Antonio
Published: August 2023

American trypanosomiasis, or Chagas disease, is caused by the protozoan parasite Trypanosoma cruzi and is characterized by the presence of cardiac or gastrointestinal symptoms in a large number of patients during the chronic phase of the disease. Although the origin of the symptoms is not clear, several mechanisms have been described involving factors related to T. cruzi and the host immune response. In this sense, the extracellular vesicles (EVs) secreted by the parasite and the immune complexes (ICs) formed after their recognition by host IgGs (EVs-IgGs) may play an important role in the immune response during infection. The aim of the present work is to elucidate the modulation of the immune response exerted by EVs and the ICs they form by analyzing the variation in the subpopulations of small and large peritoneal macrophages after intraperitoneal inoculation in mice and to evaluate the role of the sialylation of the host IgGs in this immunomodulation. Both macrophage subpopulations were purified and subjected to cytokine expression analysis by RT-qPCR. The results showed an increase in the small peritoneal macrophage subpopulation after intraperitoneal injection of parasite EVs, but a greater increase in this subpopulation was observed when sialylated and non-sialylated ICs were injected, which was similar to inoculation with the trypomastigote stage of the parasite. The cytokine expression results showed the ability of both subpopulations to express inflammatory and non-inflammatory cytokines. These results suggest the role of free EVs in the acute phase of the disease and the possible role of immune complexes in the immune response in the chronic phase of the disease, when the levels of antibodies against the parasite allow the formation of immune complexes. The differential expression of interleukins showed after the inoculation of immune complexes formed with sialylated and non-sialylated IgGs and the interleukins expression induced by EVs, demonstrates that the IgG glycosilation is involved in the type of immune response that dominates in each of the phases of the Chagas disease.
Crosstalk between hypoxic cellular micro-environment and the immune system: a potential therapeutic target for infectious diseases
Authors: Akinsulie Olalekan Chris , Shahzad Sammuel , Ogunleye Seto Charles , Oladapo Ifeoluwa Peace , Joshi Melina , Ugwu Charles Egede , Gbadegoye Joy Olaoluwa , Hassan Fasilat Oluwakemi , Adeleke Richard , Afolabi Akande Qudus , Adesola Ridwan Olamilekan
Published: August 2023

There are overwhelming reports on the promotional effect of hypoxia on the malignant behavior of various forms of cancer cells. This has been proposed and tested exhaustively in the light of cancer immunotherapy. However, there could be more interesting functions of a hypoxic cellular micro-environment than malignancy. There is a highly intricate crosstalk between hypoxia inducible factor (HIF), a transcriptional factor produced during hypoxia, and nuclear factor kappa B (NF‐κB) which has been well characterized in various immune cell types. This important crosstalk shares common activating and inhibitory stimuli, regulators, and molecular targets. Impaired hydroxylase activity contributes to the activation of HIFs. Inflammatory ligands activate NF-κB activity, which leads to the expression of inflammatory and anti-apoptotic genes. The eventual sequelae of the interaction between these two molecular players in immune cells, either bolstering or abrogating functions, is largely cell-type dependent. Importantly, this holds promise for interesting therapeutic interventions against several infectious diseases, as some HIF agonists have helped prevent immune‐related diseases. Hypoxia and inflammation are common features of infectious diseases. Here, we highlighted the role of this crosstalk in the light of functional immunity against infection and inflammation, with special focus on various innate and adaptive immune cells. Particularly, we discussed the bidirectional effects of this crosstalk in the regulation of immune responses by monocytes/macrophages, dendritic cells, neutrophils, B cells, and T cells. We believe an advanced understanding of the interplay between HIFs and NF-kB could reveal novel therapeutic targets for various infectious diseases with limited treatment options.
Copper-instigated modulatory cell mortality mechanisms and progress in oncological treatment investigations
Authors: Gao Lei , Zhang Anqi
Published: August 2023

Copper, a transition metal, serves as an essential co-factor in numerous enzymatic active sites and constitutes a vital trace element in the human body, participating in crucial life-sustaining activities such as energy metabolism, antioxidation, coagulation, neurotransmitter synthesis, iron metabolism, and tetramer deposition. Maintaining the equilibrium of copper ions within biological systems is of paramount importance in the prevention of atherosclerosis and associated cardiovascular diseases. Copper induces cellular demise through diverse mechanisms, encompassing reactive oxygen species responses, apoptosis, necrosis, pyroptosis, and mitochondrial dysfunction. Recent research has identified and dubbed a novel regulatory cell death modality—”cuprotosis”—wherein copper ions bind to acylated proteins in the tricarboxylic acid cycle of mitochondrial respiration, resulting in protein aggregation, subsequent downregulation of iron-sulfur cluster protein expression, induction of proteotoxic stress, and eventual cell death. Scholars have synthesized copper complexes by combining copper ions with various ligands, exploring their significance and applications in cancer therapy. This review comprehensively examines the multiple pathways of copper metabolism, copper-induced regulatory cell death, and the current status of copper complexes in cancer treatment.
Mercury methylation in boreal aquatic ecosystems under oxic conditions and climate change: a review
Authors: Rodríguez Juanjo
Published: August 2023

Methylmercury (MeHg) formation is a concerning environmental issue described in waters and sediments from multiple aquatic ecosystems. The genetic and metabolic bases of mercury (Hg) methylation have been well described in anoxic environments, but a number of factors seem to point towards alternative pathways potentially occurring in pelagic waters under oxic conditions. Boreal aquatic ecosystems are predicted to undergo increasing concentrations of dissolved organic matter (DOM) as a result of higher terrestrial runoff induced by climate change, which may have important implications in the formation of MeHg in the water column. In this review, different Hg methylation mechanisms postulated in the literature are discussed, with particular focus on potential pathways independent of the hgcAB gene pair and occurring under oxic conditions. Potential effects of DOM on Hg methylation and MeHg bioaccumulation are examined in the context of climate in boreal aquatic ecosystems. Furthermore, the implementation of meta-omic technologies and standardized methods into field measurements and incubation experiments is discussed as a valuable tool to determine taxonomic and functional aspects of Hg methylation in oxic waters and under climate change-induced conditions.
Simultaneously focusing electromagnetic and acoustic waves by double-physical-fields null medium
Authors: Wu Boyang , Sun Fei , Liu Yichao , Liu Xin , Zhao Xiaodan , Fei Hongming , Yang Yibiao , Chen Zhihui , Liang Shaowei , Wang Zheng , Wang Bingjie
Published: August 2023

A novel double-physical-fields lens that can simultaneously focus acoustic and electromagnetic waves into a given region is designed based on double-physical-fields null medium, which can be realized by metal plates with subwavelength separations/thicknesses and precisely designed lengths. Numerical simulations show the proposed double-physical-fields lens can create exactly the same focusing effect for both electromagnetic and acoustic waves, i.e., the same focal spot size and efficiency at the same focal length. Four typical lens with different output surfaces are studied, which shows different focusing characteristics, e.g., noodle-shaped focal spot, tiny focal spot, and capsule-shaped focal spot. With the help of the designed double-physical-fields lens, an additional degree of freedom for control can be provided by simultaneously focusing acoustic and electromagnetic waves, which may lead to wider range of applications than single-field focusing.
bettercallsal: better calling of Salmonella serotypes from enrichment cultures using shotgun metagenomic profiling and its application in an outbreak setting
Authors: Konganti Kranti , Reed Elizabeth , Mammel Mark , Kayikcioglu Tunc , Binet Rachel , Jarvis Karen , Ferreira Christina M. , Bell Rebecca L. , Zheng Jie , Windsor Amanda M. , Ottesen Andrea , Grim Christopher J. , Ramachandran Padmini
Published: August 2023

Most current Salmonella subtyping analyses rely on whole genome sequencing (WGS), which focuses on the high-resolution analysis of single genomes or multiple single genomes from the isolated colonies on microbiological agar plates. In this study, we introduce bioinformatics innovations for a metagenomic outbreak response workflow that accurately identifies multiple Salmonella serovars at the same time. bettercallsal is one of the first analysis tools to identify multiple Salmonella enterica serotypes from metagenomic or quasi-metagenomic datasets with high accuracy, allowing these isolate-independent methods to be incorporated into surveillance and root cause investigations. It was tested on an in silico benchmark dataset comprising 29 unique Salmonella serovars, 46 non-Salmonella bacterial genomes, and 10 viral genomes at varying read depths and on previously well-characterized and sequenced non-selective primary and selective enrichments of papaya and peach samples from separate outbreak investigations that resulted in the identification of multiple Salmonella serovars using traditional isolate culturing and WGS as well as nucleic acid assays. Analyses were also conducted on these datasets using a custom-built k-mer tool, SeqSero2, and Kallisto to compare serotype calling to bettercallsal. The in silico dataset analyzed with bettercallsal achieved the maximum precision, recall, and accuracy of 100, 83, and 94%, respectively. In the papaya outbreak samples, bettercallsal identified the presence of multiple serovars in agreement with the Luminex® xMAP assay results and also identified more serovars per sample, as evidenced by NCBI SNP clustering. In peach outbreak samples, bettercallsal identified two serovars in concordance with k-mer analysis and the Luminex xMAP assay. The genome hit reported by bettercallsal clustered with the chicken isolate genome, as reported by the FDA peach outbreak investigation from sequenced isolates (WGS). Overall, bettercallsal outperformed k-mer, Seqsero2, and Kallisto in identifying multiple serovars from enrichment cultures using shotgun metagenomic sequencing.
Potential role of the gut microbiota of bumblebee Bombus pyrosoma in adaptation to high-altitude habitats
Authors: Zhang Zhengyi , Guo Yulong , Zhuang Mingsheng , Liu Fugang , Xia Zhongyan , Zhang Zhihao , Yang Fan , Zeng Huayan , Wu Yueguo , Huang Jiaxing , Li Jilian
Published: August 2023

The gut microbiota affects the health and overall fitness of bumblebees. It can enhance the host's ecological range by leveraging their metabolic capacities. However, the diversity of the gut microbiota and adaptive functional evolution in high-altitude regions remain unclear. To explore how the gut microbiota helps the host adapt to high-altitude environments, we analyzed the differences in diversity and function of the gut microbiota between high- and low-altitude regions through full-length 16S rRNA sequencing. Our results show that high-altitude regions have a lower abundance of Fructobacillus and Saccharibacter compared to low-altitude regions. Additionally, some individuals in low-altitude regions were invaded by opportunistic pathogens. The gut microbiota in high-altitude regions has a greater number of pathways involved in “Protein digestion and absorption” and “Biosynthesis of amino acids,” while fewer carbohydrate pathways are involved in “digestion and absorption” and “Salmonella infection.” Our finding suggests that plateau hosts typically reduce energy metabolism and enhance immunity in response to adverse environments. Correspondingly, the gut microbiota also makes changes, such as reducing carbohydrate degradation and increasing protein utilization in response to the host. Additionally, the gut microbiota regulates their abundance and function to help the host adapt to adverse high-altitude environments.
Phage-based peptides for pancreatic cancer diagnosis and treatment: alternative approach
Authors: Li Yang , Yang Kai-di , Duan Hao-yu , Du Ya-nan , Ye Jun-feng
Published: August 2023

Pancreatic cancer is a devastating disease with a high mortality rate and a lack of effective therapies. The challenges associated with early detection and the highly aggressive nature of pancreatic cancer have limited treatment options, underscoring the urgent need for better disease-modifying therapies. Peptide-based biotherapeutics have become an attractive area of research due to their favorable properties such as high selectivity and affinity, chemical modifiability, good tissue permeability, and easy metabolism and excretion. Phage display, a powerful technique for identifying peptides with high affinity and specificity for their target molecules, has emerged as a key tool in the discovery of peptide-based drugs. Phage display technology involves the use of bacteriophages to express peptide libraries, which are then screened against a target of interest to identify peptides with desired properties. This approach has shown great promise in cancer diagnosis and treatment, with potential applications in targeting cancer cells and developing new therapies. In this comprehensive review, we provide an overview of the basic biology of phage vectors, the principles of phage library construction, and various methods for binding affinity assessment. We then describe the applications of phage display in pancreatic cancer therapy, targeted drug delivery, and early detection. Despite its promising potential, there are still challenges to be addressed, such as optimizing the selection process and improving the pharmacokinetic properties of phage-based drugs. Nevertheless, phage display represents a promising approach for the development of novel targeted therapies in pancreatic cancer and other tumors.
An improved model for target detection and pose estimation of a teleoperation power manipulator
Authors: Xie Li , Huang Jiale , Li Yutian , Guo Jianwen
Published: August 2023

Introduction

A hot cell is generally deployed with a teleoperation power manipulator to complete tests, operations, and maintenance. The position and pose of the manipulator are mostly acquired through radiation-resistant video cameras arranged in the hot cell. In this paper, deep learning-based target detection technology is used to establish an experimental platform to test the methods for target detection and pose estimation of teleoperation power manipulators using two cameras.

Methods

In view of the fact that a complex environment affects the precision of manipulator pose estimation, the dilated-fully convolutional one-stage object detection (dilated-FCOS) teleoperation power manipulator target detection algorithm is proposed based on the scale of the teleoperation power manipulator. Model pruning is used to improve the real-time performance of the dilated-FCOS teleoperation power manipulator target detection model. To improve the detection speed for the key points of the teleoperation power manipulator, the keypoint detection precision and model inference speed of different lightweight backbone networks were tested based on the SimpleBaseline algorithm. MobileNetv1 was selected as the backbone network to perform channel compression and pose distillation on the upsampling module so as to further optimize the inference speed of the model.

Results and discussion

Compared with the original model, the proposed model was experimentally proven to reach basically the same precision within a shorter inference time (only 58% of that of the original model). The experimental results show that the compressed model basically retains the precision of the original model and that its inference time is 48% of that of the original model.
Acute necrotizing encephalopathy in children with COVID-19: a retrospective study of 12 cases
Authors: Lin Xia , Wang Ying , Li Xiaoying , Abdalla Mohnad , Zhang Fan , Dong Chunhua , Lu Weifeng , Liu Xia , Zhang Jian , Ma Kang , Ma Xiang , Jiang Qin
Published: August 2023

Background

Acute necrotizing encephalopathy (ANE) is a devastating neurologic condition that can arise following a variety of systemic infections, including influenza and SARS-Cov-2. The clinical features of COVID-19-associated ANE in pediatric patients based on multi-case data have not yet been described and remain obscure. We reviewed 12 pediatric patients to better describe the clinical features of ANE with COVID-19.

Methods

We retrospectively collected and summarized the clinical features of ANE in children with COVID-19. Clinical data were collected from 12 children, including their general status, clinical symptoms, laboratory tests, and neuroimaging features.

Results

Among the subjects, 10 were over 5 years old and they accounted for 83.33%. A large percentage of those affected (66.67%) were females. The major manifestations included fever (100%), impaired consciousness (100%), and convulsions (75%). We determined that increased interleukin (IL)-6 and IL-10, and tumor necrosis factor-α and interferon gamma were not predictive of severe ANE and mortality in children with COVID-19 in this study. All children presented with abnormal neuroimaging with multiple and symmetrically distributed lesions, involving the thalamus, basal ganglia, cerebellum, and brain hemispheres. Eight of the 12 children died, resulting in a mortality rate of 66.67%, and 75% of these children were females. Importantly, we found the timely administration of mannitol after an acute onset of convulsions or disturbance of consciousness may be decreased the high mortality induced by ANE children with COVID-19.

Conclusion

COVID-19 associated with ANE in children is characterized by sudden symptom onset, rapid disease progression, and high mortality.
The effects of attention in auditory–visual integration revealed by time-varying networks
Authors: Jiang Yuhao , Qiao Rui , Shi Yupan , Tang Yi , Hou Zhengjun , Tian Yin
Published: August 2023

Attention and audiovisual integration are crucial subjects in the field of brain information processing. A large number of previous studies have sought to determine the relationship between them through specific experiments, but failed to reach a unified conclusion. The reported studies explored the relationship through the frameworks of early, late, and parallel integration, though network analysis has been employed sparingly. In this study, we employed time-varying network analysis, which offers a comprehensive and dynamic insight into cognitive processing, to explore the relationship between attention and auditory-visual integration. The combination of high spatial resolution functional magnetic resonance imaging (fMRI) and high temporal resolution electroencephalography (EEG) was used. Firstly, a generalized linear model (GLM) was employed to find the task-related fMRI activations, which was selected as regions of interesting (ROIs) for nodes of time-varying network. Then the electrical activity of the auditory-visual cortex was estimated via the normalized minimum norm estimation (MNE) source localization method. Finally, the time-varying network was constructed using the adaptive directed transfer function (ADTF) technology. Notably, Task-related fMRI activations were mainly observed in the bilateral temporoparietal junction (TPJ), superior temporal gyrus (STG), primary visual and auditory areas. And the time-varying network analysis revealed that V1/A1↔STG occurred before TPJ↔STG. Therefore, the results supported the theory that auditory-visual integration occurred before attention, aligning with the early integration framework.
From pulse width modulated TENS to cortical modulation: based on EEG functional connectivity analysis
Authors: Jadidi Armita Faghani , Jensen Winnie , Zarei Ali Asghar , Lontis Eugen Romulus , Atashzar S. Farokh
Published: August 2023

Modulation in the temporal pattern of transcutaneous electrical nerve stimulation (TENS), such as Pulse width modulated (PWM), has been considered a new dimension in pain and neurorehabilitation therapy. Recently, the potentials of PWM TENS have been studied on sensory profiles and corticospinal activity. However, the underlying mechanism of PWM TENS on cortical network which might lead to pain alleviation is not yet investigated. Therefore, we recorded cortical activity using electroencephalography (EEG) from 12 healthy subjects and assessed the alternation of the functional connectivity at the cortex level up to an hour following the PWM TENS and compared that with the effect of conventional TENS. The connectivity between eight brain regions involved in sensory and pain processing was calculated based on phase lag index and spearman correlation. The alteration in segregation and integration of information in the network were investigated using graph theory. The proposed analysis discovered several statistically significant network changes between PWM TENS and conventional TENS, such as increased local strength and efficiency of the network in high gamma-band in primary and secondary somatosensory sources one hour following stimulation. Our findings regarding the long-lasting desired effects of PWM TENS support its potential as a therapeutic intervention in clinical research.
Synthetic approaches to cis-THC, a promising scaffold in medicinal chemistry
Authors: Gurgone Lucía , La-Venia Agustina , Caprioglio Diego , Riveira Martín J.
Published: August 2023

The chemistry of phytocannabinoids has witnessed renewed interest these last decades as a consequence of reduced restrictions, research on the endocannabinoid system and the development of approved therapeutic treatments based on cannabinoids. The medicinal cannabinoid market constitutes a prolific scenario in current medicine. Most studies, however, have focused on only two major components of Cannabis sativa L., namely, cannabidiol (CBD, 2) and (−)-Δ9-trans-tetrahydrocannabinol (Δ9-trans-THC, 6a), the latter being the main psychoactive compound of this plant. The cis-diastereoisomer of Δ9-trans-THC, Δ9-cis-THC, although also present in the same plant, has been less investigated in terms of biological, medicinal and synthetic perspectives. Interestingly, the cis-fused tetrahydrobenzo [c]chromene motif present in Δ9-cis-THC is embedded in many other natural products which also exhibit interesting biological activities such as anticancer, antifungal, and antiparasitic. This review discloses synthetic approaches that have been established towards the cis-fused tetrahydroisochromene system of Δ9-cis-THC.
MicroRNAs miR-16 and miR-519 control meningioma cell proliferation via overlapping transcriptomic programs shared with the RNA-binding protein HuR
Authors: Hergalant Sébastien , Casse Jean-Matthieu , Oussalah Abderrahim , Houlgatte Rémi , Helle Déborah , Rech Fabien , Vallar Laurent , Guéant Jean-Louis , Vignaud Jean-Michel , Battaglia-Hsu Shyue-Fang , Gauchotte Guillaume
Published: August 2023

Introduction

Meningiomas are the most common type of primary central nervous system tumors. In about 80% cases, these tumors are benign and grow very slowly, but the remainder 20% can unlock higher proliferation rates and become malignant. In this study we examined two miRs, miR-16 and miR-519, and evaluated their role in tumorigenesis and cell growth in human meningioma.

Methods

A cohort of 60 intracranial grade 1 and grade 2 human meningioma plus 20 healthy meningeal tissues was used to quantify miR-16 and miR-519 expressions. Cell growth and dose-response assays were performed in two human meningioma cell lines, Ben-Men-1 (benign) and IOMM-Lee (aggressive). Transcriptomes of IOMM-lee cells were measured after both miR-mimics transfection, followed by integrative bioinformatics to expand on available data.

Results

In tumoral tissues, we detected decreased levels of miR-16 and miR-519 when compared with arachnoid cells of healthy patients (miR-16: P=8.7e-04; miR-519: P=3.5e-07). When individually overexpressing these miRs in Ben-Men-1 and IOMM-Lee, we observed that each showed reduced growth (P<0.001). In IOMM-Lee cell transcriptomes, downregulated genes, among which ELAVL1/HuR (miR-16: P=6.1e-06; miR-519:P=9.38e-03), were linked to biological processes such as mitotic cell cycle regulation, pre-replicative complex, and brain development (FDR<1e-05). Additionally, we uncovered a specific transcriptomic signature of miR-16/miR-519-dysregulated genes which was highly enriched in HuR targets (>6-fold; 79.6% of target genes).

Discussion

These results were confirmed on several public transcriptomic and microRNA datasets of human meningiomas, hinting that the putative tumor suppressor effect of these miRs is mediated, at least in part, via HuR direct or indirect inhibition.
Genetics of enzymatic dysfunctions in metabolic disorders and cancer
Authors: Mahé Mélanie , Rios-Fuller Tiffany J. , Karolin Andrea , Schneider Robert J.
Published: August 2023

Inherited metabolic disorders arise from mutations in genes involved in the biogenesis, assembly, or activity of metabolic enzymes, leading to enzymatic deficiency and severe metabolic impairments. Metabolic enzymes are essential for the normal functioning of cells and are involved in the production of amino acids, fatty acids and nucleotides, which are essential for cell growth, division and survival. When the activity of metabolic enzymes is disrupted due to mutations or changes in expression levels, it can result in various metabolic disorders that have also been linked to cancer development. However, there remains much to learn regarding the relationship between the dysregulation of metabolic enzymes and metabolic adaptations in cancer cells. In this review, we explore how dysregulated metabolism due to the alteration or change of metabolic enzymes in cancer cells plays a crucial role in tumor development, progression, metastasis and drug resistance. In addition, these changes in metabolism provide cancer cells with a number of advantages, including increased proliferation, resistance to apoptosis and the ability to evade the immune system. The tumor microenvironment, genetic context, and different signaling pathways further influence this interplay between cancer and metabolism. This review aims to explore how the dysregulation of metabolic enzymes in specific pathways, including the urea cycle, glycogen storage, lysosome storage, fatty acid oxidation, and mitochondrial respiration, contributes to the development of metabolic disorders and cancer. Additionally, the review seeks to shed light on why these enzymes represent crucial potential therapeutic targets and biomarkers in various cancer types.