bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling of Personalized Treatments in Colon Cancer Based on Preclinical Genomic and Drug Sensitivity Data
Authors: Keil Marlen , Conrad Theresia , Becker Michael , Keilholz Ulrich , Yaspo Marie-Laure , Lehrach Hans , Schütte Moritz , Haybaeck Johannes , Hoffmann Jens
Published: November 2021

The current standard therapies for advanced, recurrent or metastatic colon cancer are the 5-fluorouracil and oxaliplatin or irinotecan schedules (FOxFI) +/− targeted drugs cetuximab or bevacizumab. Treatment with the FOxFI cytotoxic chemotherapy regimens causes significant toxicity and might induce secondary cancers. The overall low efficacy of the targeted drugs seen in colon cancer patients still is hindering the substitution of the chemotherapy. The ONCOTRACK project developed a strategy to identify predictive biomarkers based on a systems biology approach, using omics technologies to identify signatures for personalized treatment based on single drug response data. Here, we describe a follow-up project focusing on target-specific drug combinations. Background for this experimental preclinical study was that, by analyzing the tumor growth inhibition in the PDX models by cetuximab treatment, a broad heterogenic response from complete regression to tumor growth stimulation was observed. To provide confirmation of the hypothesis that drug combinations blocking alternatively activated oncogenic pathways may improve therapy outcomes, 25 models out of the well-characterized ONCOTRACK PDX panel were subjected to treatment with a drug combination scheme using four approved, targeted cancer drugs.
Photo-Fenton Oxidation of Methyl Orange Dye Using South African Ilmenite Sands as a Catalyst
Authors: Butt Alicia Levana , Mpinga John Kabangu , Tichapondwa Shepherd Masimba
Published: November 2021

In this study, the viability of South African ilmenite sands as a catalyst in the photo-Fenton-like degradation of methyl orange (MO) dye was investigated. The mineralogy and other properties of the material were characterized. Complete decolorization occurred under acidic conditions (pH < 4) in the presence of ilmenite and H2O2. Light irradiation accelerated the rate of reaction. Parameter optimization revealed that a pH of 2.5, UVB irradiation, 2 g/L catalyst loading, and a hydrogen peroxide concentration of 1.0 mM were required. Under these conditions, complete decolorization was observed after 45 min. Degradation kinetics were best described by the pseudo-first order (PFO) model. Rate constants of 0.095 and 0.034 min−1 were obtained for 5 and 20 mg/L MO concentrations, respectively. A 37% total organic carbon removal was observed after 60 min. This suggests a stepwise MO degradation pathway with intermediate formation rather than complete mineralization. Although iron leaching was detected, the mineralogy of the catalyst recovered after the reaction was similar to the fresh catalyst.
Reaction Mechanism of the Microwave-Assisted Synthesis of 5-Hydroxymethylfurfural from Sucrose in Sugar Beet Molasses
Authors: Rocha Sebastián , Marzialetti Teresita , Kopp Matías , Cea Mara
Published: November 2021

5-hydroxymethylfurfural (HMF) stands out among the chemical products derived from biomass as a building block in the chemical industry. The conventional production of HMF is usually carried out from fructose, glucose, or other monosaccharides as feedstock, but sugar beet molasses, a by-product of the sugar industry containing sucrose (45–55%), is promising. This exploratory study used three aqueous stock solutions and one biphasic system as the sources of sucrose. The dehydration of sucrose to 5-hydroxymethylfurfural was assisted by microwave heating and subcritical water conditions. The maximum yield of HMF was 27.8 mol % for the aqueous solution of synthetic sucrose at 80 min of treatment. Although HMF yield was 7.1 mol % in the aqueous sugar beet molasses solution, it increased 2-fold after clarification (15.1 mol %) and 1.6-fold in the biphasic system (11.4 mol %). These are favorable outcomes since this is an exploratory investigation. The pseudo-first-order model fitted experimental data from the conversion of the sucrose from the stock solutions, and kinetic parameters were estimated and compared. The estimated reaction rate constant showed that inversion of sucrose is faster than fructose dehydration to HMF, but the latter reaction was the rate-determining step only for the biphasic system. The maximum partition coefficient value was four between 40 min and 60 min of reaction, calculated at room temperature. These predictions help investigators to estimate conversions and selectivity when pilot plants need to be simulated.
The Abscission Checkpoint: A Guardian of Chromosomal Stability
Authors: Petsalaki Eleni , Zachos George
Published: November 2021

The abscission checkpoint contributes to the fidelity of chromosome segregation by delaying completion of cytokinesis (abscission) when there is chromatin lagging in the intercellular bridge between dividing cells. Although additional triggers of an abscission checkpoint-delay have been described, including nuclear pore defects, replication stress or high intercellular bridge tension, this review will focus only on chromatin bridges. In the presence of such abnormal chromosomal tethers in mammalian cells, the abscission checkpoint requires proper localization and optimal kinase activity of the Chromosomal Passenger Complex (CPC)-catalytic subunit Aurora B at the midbody and culminates in the inhibition of Endosomal Sorting Complex Required for Transport-III (ESCRT-III) components at the abscission site to delay the final cut. Furthermore, cells with an active checkpoint stabilize the narrow cytoplasmic canal that connects the two daughter cells until the chromatin bridges are resolved. Unsuccessful resolution of chromatin bridges in checkpoint-deficient cells or in cells with unstable intercellular canals can lead to chromatin bridge breakage or tetraploidization by regression of the cleavage furrow. In turn, these outcomes can lead to accumulation of DNA damage, chromothripsis, generation of hypermutation clusters and chromosomal instability, which are associated with cancer formation or progression. Recently, many important questions regarding the mechanisms of the abscission checkpoint have been investigated, such as how the presence of chromatin bridges is signaled to the CPC, how Aurora B localization and kinase activity is regulated in late midbodies, the signaling pathways by which Aurora B implements the abscission delay, and how the actin cytoskeleton is remodeled to stabilize intercellular canals with DNA bridges. Here, we review recent progress toward understanding the mechanisms of the abscission checkpoint and its role in guarding genome integrity at the chromosome level, and consider its potential implications for cancer therapy.
Associations of Genes for Killer Cell Immunoglobulin-like Receptors and Their Human Leukocyte Antigen-A/B/C Ligands with Abdominal Aortic Aneurysm
Authors: Dubis Joanna , Niepiekło-Miniewska Wanda , Jędruchniewicz Natalia , Sobczyński Maciej , Witkiewicz Wojciech , Zapotoczny Norbert , Kuśnierczyk Piotr
Published: November 2021

Abdominal aortic aneurysm (AAA) is an immune-mediated disease with a genetic component. The multifactorial pathophysiology is not clear and there is still no pharmacotherapy to slow the growth of aneurysms. The signal integration of cell-surface KIRs (killer cell immunoglobulin-like receptors) with HLA (ligands, human leukocyte class I antigen molecules) modulates the activity of natural killer immune cells. The genetic diversity of the KIR/HLA system is associated with the risk of immune disorders. This study was a multivariate analysis of the association between genetic variants of KIRs, HLA ligands, clinical data and AAA formation. Genotyping was performed by single polymerase chain reaction with sequence-specific primers using commercial assays. Patients with HLA-A-Bw4 have a larger aneurysm by an average of 4 mm (p = 0.008). We observed a relationship between aneurysm diameter and BMI in patients with AAA and co-existing CAD; its shape was determined by the presence of HLA-A-Bw4. There was also a nearly 10% difference in KIR3DL1 allele frequency between the study and control groups. High expression of the cell surface receptor KIR3DL1 may protect, to some extent, against AAA. The presence of HLA-A-Bw4 may affect the rate of aneurysm growth and represents a potential regional pathogenetic risk of autoimmune injury to the aneurysmal aorta.
Effectiveness of Chloral Hydrate on Brain MRI in Children with Developmental Delay/Intellectual Disability Comparing with Normal Intelligence: Single Tertiary Center Experience
Authors: Moon Ja Un , Han Ji Yoon
Published: November 2021

Neurodiagnostic investigation requirements are expanding for diagnostic and therapeutic purposes in children, especially in those with developmental delay/intellectual disability (DD/ID). Thus, determination of optimal sedatives to achieve successful sedation and immobility without further neurological compromise is important in children with DD/ID. The purpose of this study is to assess the effectiveness and adverse reactions of chloral hydrate (CH) for brain magnetic resonance imaging (B-MRI) in children with DD/ID compared to those with normal intelligence (NI). We performed a retrospective chart review of children aged from 1 day to 12 years who required elective sedation using CH for B-MRI. About 730 cases (415 with DD/ID and 315 with NI) of CH sedation were conducted for B-MRI. Children with DD/ID showed a higher failure rate (22%) than did those with NI (6%); additional CH and prolonged sedation time were required. There was no difference in incidence of adverse reactions between DD/ID and NI groups (p = 0.338). Older or heavier children with DD/ID (p = 0.036 and p = 0.013, respectively), as well as those diagnosed with epilepsy or neuropsychiatric disorders showed higher risk of sedation failure (p < 0.001 for each). In conclusion, CH was a suboptimal sedative drug for children with DD/ID compared with those with NI. Other alternative or supplementary sedatives should be taken into consideration especially for those vulnerable groups.
Effectiveness and Acceptability of Telerehabilitation in Physical Therapy during COVID-19 in Children: Findings of a Systematic Review
Authors: Alonazi Asma
Published: November 2021

The goal of this systematic review was to determine the efficacy and acceptability of telerehabilitation in physical therapy (PT) and parental acceptance during the COVID-19 pandemic in children. In 2021, an electronic search of academic articles was performed using databases such as Embase, PubMed and Scopus. One-hundred and one articles did not satisfy the eligibility criteria after deleting duplicates and reviewing abstracts, while 16 papers did not meet eligibility after reviewing complete texts. Hence, full texts for 13 articles were retrieved, which were incorporated in the review. All these studies were observational studies assessing the effectiveness and acceptability of telerehabilitation in PT required for diverse conditions in children during the COVID-19 pandemic. All included studies revealed a positive effect of telerehabilitation in PT during the COVID-19 pandemic in children with different conditions. Moreover, the included studies revealed that both rehabilitation professionals and parents or caregivers of children were satisfied with the telerehabilitation services provided remotely. Thus, telerehabilitation appears to be a suitable and convenient strategy to offer remote services to children in need but cannot visit in person due to COVID-19. The existing evidence shows that telerehabilitation can be considered effective for children who need PT for any health condition mainly during the pandemic. However, due to the dearth of studies in this area, exploring this topic is recommended mostly in low-middle-income countries with poor access to health care services and limited resources.
Bacterial Adhesion on Prosthetic and Orthotic Material Surfaces
Authors: Abram Anže , Zore Anamarija , Lipovž Urban , Košak Anita , Gavras Maja , Boltežar Žan , Bohinc Klemen
Published: November 2021

Prosthetic and orthotic parts, such as prosthetic socket and inner sides of orthoses, are often in contact with human skin, giving bacteria the capability to adhere and form biofilms on the materials of those parts which can further cause infections. The purpose of this study was to determine the extent of bacterial adhesion of Staphylococcus aureus and Staphylococcus epidermidis on twelve different prosthetic and orthotic material surfaces and how roughness, hydrophobicity, and surface charge of this materials affect the adhesion. The roughness, contact angle, zeta potential of material surfaces, and adhesion rate of Staphylococcus aureus and Staphylococcus epidermidis were measured on all twelve prosthetic and orthotic materials, i.e., poly(methyl methacrylate), thermoplastic elastomer, three types of ethylene polyvinyl acetates (pure, with low-density polyethylene and with silver nanoparticles), silicone, closed-cell polyethylene foams with and without nanoparticles, thermo and natural cork, and artificial and natural leather. The greatest degree of adhesion was measured on both closed-cell polyethylene foams, followed by artificial thermo cork and leather. The lowest adhesion extent was observed on ethylene-vinyl acetate. The bacterial adhesion extent increases with the increasing surface roughness. Smaller deviations of this rule are the result of the surface’s hydrophobicity and charge.
MHD 3D Crossflow in the Streamwise Direction Induced by Nanofluid Using Koo–Kleinstreuer and Li (KLL) Correlation
Authors: Khan Umair , Bouslimi Jamel , Zaib Aurang , Al-Mubaddel Fahad S. , Imtiaz Najma , Alharbi Abdulaziz N. , Eid Mohamed R.
Published: November 2021

Aluminum nanoparticles are suitable for wiring power grids, such as local power distribution and overhead power transmission lines, because they exhibit high conductivity. These nanoparticles are also among the most utilized materials in electrical field applications. Thus, the present study investigated the impact of magnetic field on 3D crossflow in the streamwise direction with the impacts of Dufour and Soret. In addition, the effects of activation energy and chemical reaction were incorporated. The viscosity and thermal conductivity of nanofluids were premeditated by KKL correlation. Prominent PDEs (Partial Differential Equations) were converted into highly nonlinear ODEs (Ordinary Differential Equations) using the proper similarity technique and then analyzed numerically with the aid of the built-in bvp4c solver in MATLAB. The impact of diverse important variables on temperature and velocity was graphically examined. Additionally, the influences of pertaining parameters on the drag force coefficient, Nusselt number, and Sherwood number were investigated. Inspections revealed that the mass transfer rate decreases, while the heat transport increases with increasing values of the Soret factor. However, the Nusselt and Sherwood numbers validate the differing trend for rising quantities of the Dufour factor.
Unimodal and Multimodal Perception for Forest Management: Review and Dataset
Authors: da Silva Daniel Queirós , dos Santos Filipe Neves , Sousa Armando Jorge , Filipe Vítor , Boaventura-Cunha José
Published: November 2021

Robotics navigation and perception for forest management are challenging due to the existence of many obstacles to detect and avoid and the sharp illumination changes. Advanced perception systems are needed because they can enable the development of robotic and machinery solutions to accomplish a smarter, more precise, and sustainable forestry. This article presents a state-of-the-art review about unimodal and multimodal perception in forests, detailing the current developed work about perception using a single type of sensors (unimodal) and by combining data from different kinds of sensors (multimodal). This work also makes a comparison between existing perception datasets in the literature and presents a new multimodal dataset, composed by images and laser scanning data, as a contribution for this research field. Lastly, a critical analysis of the works collected is conducted by identifying strengths and research trends in this domain.
Transient Two-Way Molecular-Continuum Coupling with OpenFOAM and MaMiCo: A Sensitivity Study
Authors: Wittenberg Helene , Neumann Philipp
Published: November 2021

Molecular-continuum methods, as considered in this work, decompose the computational domain into continuum and molecular dynamics (MD) sub-domains. Compared to plain MD simulations, they greatly reduce computational effort. However, the quality of a fully two-way coupled simulation result strongly depends on a variety of system-specific parameters, and the corresponding sensitivity is only rarely addressed in the literature. Using a state-flux molecular-continuum coupling algorithm, we investigated the influences of various parameters, such as the size of the overlapping region, the coupling time step and the quality of ensemble-based sampling of flow velocities, in a Couette flow scenario. In particular, we considered a big setup in terms of domain size and number of time steps, which allowed us to investigate the long-term behavior of the coupling algorithm close to the incompressible regime. While mostly good agreement was reached on short time scales, it was the long-term behavior which differed even with slightly differently parametrized simulations. We demonstrated our findings by measuring the error in velocity, and we summarize our main observations with a few lessons learned.
IoT Security Mechanisms in the Example of BLE
Authors: Kalinin Evgeny , Belyakov Danila , Bragin Dmitry , Konev Anton
Published: November 2021

In recent years, a lot of IoT devices, wireless sensors, and smart things contain information that must be transmitted to the server for further processing. Due to the distance between devices, battery power, and the possibility of sudden device failure, the network that connects the devices must be scalable, energy efficient, and flexible. Particular attention must be paid to the protection of the transmitted data. The Bluetooth mesh was chosen as such a network. This network is built on top of Bluetooth Low-Energy devices, which are widespread in the market and whose radio modules are available from several manufacturers. This paper presents an overview of security mechanisms for the Bluetooth mesh network. This network provides encryption at two layers: network and upper transport layers, which increases the level of data security. The network uses sequence numbers for each message to protect against replay attacks. The introduction of devices into the network is provided with an encryption key, and the out-of-band (OOB) mechanism is also supported. At the moment, a comparison has been made between attacks and defense mechanisms that overlap these attacks. The article also suggested ways to improve network resiliency.
Microstructure Study of Phase Transformation of Quartz in Potassium Silicate Glass at 900 °C and 1000 °C
Authors: Li Wenbo , Xu Chenghao , Xie Ameng , Chen Ken , Yang Yingfei , Liu Lanlan , Zhu Shenglong
Published: November 2021

Interfacial reaction between quartz and potassium silicate glass was studied at both 900 °C and 1000 °C. The results showed that no phase transformation was observed for the pure quartz at 900 °C or 1000 °C. Instead, for quartz particles in K2O-SiO2 glass, the transformation from quartz to cristobalite occurred at the quartz/glass interface at first, and then the cristobalite crystals transformed into tridymite. The tridymite formed at the interface between particles and glass became the site of heterogeneous nucleation, which induces plenty of tridymite precipitation in potassium silicate glass. The influential mechanism of firing temperature and size of quartz particles on transformation rate was discussed.
Fatty Liver Index for Predicting Nonalcoholic Fatty Liver Disease in an Asymptomatic Korean Population
Authors: Cho Eun-Ju , Jung Gu-Cheol , Kwak Min-Sun , Yang Jong-In , Yim Jeong-Yoon , Yu Su-Jong , Chung Goh-Eun
Published: November 2021

Nonalcoholic fatty liver disease (NAFLD) is increasing worldwide, highlighting the importance of early and accurate detection and the appropriate management of NAFLD. However, ultrasonography (US) is not included in many mass screening programs, and people have limited access to it. The aim of this study is to validate the fatty liver index (FLI) and investigate the optimal cutoff value for predicting NAFLD in an asymptomatic population. We conducted a retrospective cohort study in Korea. All subjects who underwent health checkup exams, including abdominal US, controlled attenuation parameter (CAP) and blood testing, were enrolled. Analyses of the area under the receiver operating characteristic curve (AUROC) were used to evaluate the diagnostic accuracy and to calculate the optimal FLI cutoff for US-NAFLD. Among the 4009 subjects (mean age 54.9 years, 83.5% male), the prevalence of US-diagnosed NAFLD and CAP-defined hepatic steatosis was 61.4% and 55.4%. The previously used cutoff of FLI = 60 showed poor performance in predicting US-diagnosed NAFLD, with an AUROC of 0.63 (0.62–0.64), and CAP-defined NAFLD, with an AUROC 0.63 (0.62–0.64). The optimal FLI cutoff values to discriminate fatty liver detected by US were 29 for the entire population, with an AUROC of 0.82 (0.81–0.84). The sex-specific values were 31 for males and 18 for females (sensitivity 72.8% and 73.4%; specificity 74.2% and 85.0%, respectively). The FLI cutoff for US-diagnosed NAFLD can be set as 29 for the entire Korean population. Considering the sex dimorphism in NAFLD, different cutoff values are suggested to predict US-diagnosed NAFLD. These results may be helpful in the accurate non-invasive diagnosis of NAFLD.
Isolation and Enrichment of Circulating Fetal Cells for NIPD: An Overview
Authors: Sabbatinelli Giulia , Fantasia Donatella , Palka Chiara , Morizio Elisena , Alfonsi Melissa , Calabrese Giuseppe
Published: November 2021

Prenatal diagnosis plays a crucial role in clinical genetics. Non-invasive prenatal diagnosis using fetal cells circulating in maternal peripheral blood has become the goal of prenatal diagnosis, to obtain complete fetal genetic information and avoid risks to mother and fetus. The development of high-efficiency separation technologies is necessary to obtain the scarce fetal cells from the maternal circulation. Over the years, multiple approaches have been applied, including choice of the ideal cell targets, different cell recovering technologies, and refined cell isolation yield procedures. In order to provide a useful tool and to give insights about limitations and advantages of the technologies available today, we review the genetic research on the creation and validation of non-invasive prenatal diagnostic testing protocols based on the rare and labile circulating fetal cells during pregnancy.
Importance of Visual Estimation of Coronary Artery Stenoses and Use of Functional Evaluation for Appropriate Guidance of Coronary Revascularization—Multiple Operator Evaluation
Authors: Calmac Lucian , Popa-Fotea Nicoleta-Monica , Bataila Vlad , Ploscaru Vlad , Turea Adrian , Tache Irina Andra , Stoian Diana , Itu Lucian , Badila Elisabeta , Scafa-Udriste Alexandru , Dorobantu Maria
Published: November 2021

Background: Visual estimation (VE) of coronary stenoses is the first step during invasive coronary angiography. The aim of this study was to evaluate the accuracy of VE together with invasive functional assessment (IFA) in defining the functional significance (FS) of coronary stenoses based on the opinion of multiple operators. Methods: Fourteen independent operators visually evaluated 133 coronary lesions which had a previous FFR measurement, indicating the degree of stenosis (DS), FS and IFA intention. We determined the accuracy of FS prediction using several scenarios combining individual and group decision, considering IFA as deemed necessary by the operator or only in intermediate lesions. Results: The accuracy of VE in predicting FS was largely variable between operators (average 66.1%); it improved significantly when IFA was used either as per operator’s opinion (86.3%; p < 0.0001) or only in intermediate DS (82.9; p < 0.0001). There was no significant difference between using IFA per observer’s opinion or only in intermediate DS lesions (p = 0.166). The poorest accuracy of VE for FS was obtained in intermediate DS lesions (59.1%). Conclusions: There are significant inter-observer differences in reporting the degree of DS, while the accuracy of VE prediction of FS is also largely dependent on the operator, and the worst performance is obtained in the evaluation of intermediate DS.
How Kindergarten Teachers Assess Their Own Professional Competencies
Authors: Pupíková Eva , Gonda Dalibor , Páleníková Kitti , Medová Janka , Kolárová Dana , Tirpáková Anna
Published: November 2021

One of the requirements of Education 4.0 is that students and practitioners should be involved in the creation of the content of study plans. Therefore, in the present research we focused on identifying the further educational needs of kindergarten teachers. Teachers’ educational needs were divided into four dimensions: ‘content knowledge’, ‘diagnostic knowledge’, ‘didactical knowledge’, and ‘classroom management knowledge’. In parallel, we discovered how teachers assess the level of their own teaching competencies. Based on the obtained data, we identified that teachers have the greatest need for further education in the dimension of ‘diagnostic knowledge’ and that the need for their further education in this dimension did not depend on the length of practice. In the other three dimensions, a declining trend in teachers’ educational needs has been recorded with an increasing length of practice, declining significantly in three of the four dimensions examined. The study points to the need to create in-service courses for kindergarten teachers to deepen their ‘diagnostic knowledge’ and thus ensure the sustainability of the quality of pre-school education for children. Teachers‘ self-assessment of their own teaching competencies corresponds to their educational needs, which supports the relevance of the findings on the further educational needs of kindergarten teachers. This study aimed to obtain relevant data on which the improvement of the higher education of future kindergarten teachers might be based. At the same time, this would allow the analysis and tailoring of the content of professional development courses to the needs of in-service kindergarten teachers.
An All-Textile Dual-Band Antenna for BLE and LoRa Wireless Communications
Authors: Ibrahim Nur Fatihah , Dzabletey Philip Ayiku , Kim Hyoungsoo , Chung Jae-Young
Published: November 2021

In this paper, a dual-band conductive textile-based wearable antenna operating at LoRa-868 MHz and BLE-2.4 GHz is presented. The proposed antenna is intended for accurate geolocation, tracking and communication applications in the military, industrial and telemedicine industries. The low-profile patch antenna is suitable for integrating into clothing. It is composed of three textile layers: top and bottom silver-ink-printed polystyrene fabrics, and a neoprene substrate. To utilize the flexible and restorable properties of these textile materials, the proposed antenna is directly fed by a flexible cable using an aperture-coupled feeding technique. This method not only eliminates the use of the conventional, bulky, and metallic SMA connector but also introduces a secondary resonance at 2.4 GHz, enabling the dual-band property. Using a thin coaxial cable fixed on the aperture slot for proximity coupling, a compact antenna size of 150 mm2 is obtained that can easily be attached and detached on existing cloths. The proposed structure has been fabricated and measured in an anechoic chamber to verify the performance. Measured gain of 3.28 dBi and 3.25 dBi was realized for LoRa and BLE at an antenna size of 0.61 λg × 0.61 λg × 0.012 λg (where λg is guided wavelength at 868 MHz) with a front-to-back ratio (FBR) of greater than 10 dBi.
Measuring the Damping Performance of Gradient-Structured Bamboo Using the Resonance Method
Authors: Chen Xiaoyi , Deng Liping , Wei Xin , Li Mingpeng , Wang Ge , Chen Fuming
Published: November 2021

Bamboo has natural damping properties, but, due to the obvious gradient differences in bamboo walls, the damping properties of different layers may vary. Using bamboo slivers as the research object, this study investigated the underlying mechanism of the effect of microstructural and chemical components on the damping properties (η, damping ratio) of bamboo using the resonance and nonresonance methods. The damping ratio decreased on L3 (inner layer), L2 (middle layer), and L1 (outer layer) due to lower microfibril angles, increased crystallinity of cellulose, and decreased hemicellulose content. All of these limited the motion of the bamboo’s molecular chains. The damping ratio successively increased in the oven-dried, air-dried, and water saturated states because water acted as a plasticizer. The damping ratio of L1, in the oven-dried state, was slightly higher than that of the air-dried state because L1 had the lowest water content. This allowed less water to escape during drying, which intensified the molecular distortion. The initial tan δ (tangent of the loss angle) decreased successively on the L3, L2, and L1 layers of the bamboo, and the tan δ of L3 was lower than that of L2 due to changes in the temperature sensitivity of hemicellulose.
The Emergence and Identification of Large-Scale Coherent Structures in Free Convective Flows of the Rayleigh-Bénard Type
Authors: Smirnov Sergei , Smirnovsky Alexander , Bogdanov Sergey
Published: November 2021

The revealing of the turbulence archetypes is one of the fundamental problems in the study of turbulence, which is important not only from the fundamental point of view but also for practical applications, e.g., in geophysics of ocean and lakes. The paper is devoted to the study of the emergence of coherent structures and the identification of their turbulent archetypes, typical for the free convective flows of the Rayleigh-Bénard type. Using Direct Numerical Simulation, we perform a numerical study of two refined convective flows: convection in a cylinder heated from below and internally heated convection in a layer. The main purpose of the study is identifying coherent structures (CS), investigating its main features and properties, and determining the turbulence archetypes using the anisotropy invariant map (AIM). We show that, in both configurations considered, CS takes place. In a cylinder, CS is a single large-scale vortex that can rotate azimuthally in non-titled container, but is almost “fixed” in the case of slightly tilted cylinder; in a layer, CS is a quasi-2D vortex, which can arise, exist for some time, disrupt, and then re-emerge again in the orthogonal direction. Nevertheless, the turbulence archetypes represented by the AIM are quite similar for both cases, and there are the distinct CS fingerprints on AIM.
Raman Fingerprints of Rice Nutritional Quality: A Comparison between Japanese Koshihikari and Internationally Renowned Cultivars
Authors: Pezzotti Giuseppe , Zhu Wenliang , Hashimoto Yuuki , Marin Elia , Masumura Takehiro , Sato Yo-Ichiro , Nakazaki Tetsuya
Published: November 2021

Raman spectroscopy was applied to characterize at the molecular scale the nutritional quality of the Japanese Koshihikari rice cultivar in comparison with other renowned rice cultivars including Carnaroli from Italy, Calrose from the USA, Jasmine rice from Thailand, and Basmati from both India and Pakistan. For comparison, two glutinous (mochigome) cultivars were also investigated. Calibrated and validated Raman analytical algorithms allowed quantitative determinations of: (i) amylopectin and amylose concentrations, (ii) fractions of aromatic amino acids, and (iii) protein content and secondary structure. The Raman assessments non-destructively linked the molecular composition of grains to key nutritional parameters and revealed a complex intertwine of chemical properties. The Koshihikari cultivar was rich in proteins (but with low statistical relevance as compared to other investigated cultivars) and aromatic amino acids. However, it also induced a clearly higher glycemic impact as compared to long-grain cultivars from Asian countries. Complementary to genomics and wet-chemistry analyses, Raman spectroscopy makes non-destructively available factual and data-driven information on rice nutritional characteristics, thus providing customers, dietitian nutritionists, and producers with a solid science-consolidated platform.
Content and Bioaccessibility of Vitamin K (Phylloquinone and Menaquinones) in Cheese
Authors: Jensen Marie Bagge , Daugintis Andrius , Jakobsen Jette
Published: November 2021

Vitamin K is a fat-soluble group of vitamers consisting of phylloquinone (PK) and menaquinones (MKs). To date, only a daily reference intake for PK is set; however, in the last decade, research studying the correlation between MKs intake and improvement of health in regards to cardiovascular diseases, bone metabolism, and chronic kidney disease has been conducted. MKs are synthesised by bacteria in the fermentation process of foods, e.g., cheeses. The content and bioaccessibility of vitamin K vitamers (PK, MK-4, MK-5, MK-6, MK-7, MK-8, MK-9, and MK-10) were assessed in eight different cheese products differing in ripening time, starter culture, fat content, and water content. The bioaccessibility was assessed using the static in vitro digestion model INFOGEST 2.0. Variation of the vitamin K content (<0.5 μg/100 g–32 μg/100 g) and of the vitamin K bioaccessibility (6.4–80%) was observed. A longer ripening time did not necessarily result in an increase of MKs. These results indicate that the vitamin K content and bioaccessibility differs significantly between different cheese products, and the ripening time, starter culture, fat content, and water content cannot explain this difference.
Compositional Features and Nutritional Value of Pig Brain: Potential and Challenges as a Sustainable Source of Nutrients
Authors: Chanted Jaruwan , Panpipat Worawan , Panya Atikorn , Phonsatta Natthaporn , Cheong Ling-Zhi , Chaijan Manat
Published: November 2021

The goal of this study was to establish the nutritional value and compositional properties of the brains of crossbred pigs (Landrace–Large white–Duroc (LLD)), in order to realize the zero-waste concept and increase the use of by-products in the sustainable meat industry. Fat (9.25% fresh weight (fw)) and protein (7.25% fw) were the principal dry matters of pig brain, followed by carbohydrate and ash. Phospholipid and cholesterol had a 3:1 ratio. Pig brain had a red tone (L* = 63.88, a* = 5.60, and b* = 15.43) and a high iron content (66 mg/kg) due to a total heme protein concentration of 1.31 g/100 g fw. The most prevalent macro-element was phosphorus (14 g/kg), followed by potassium, sodium, calcium, and magnesium. Zinc, copper, and manganese were among the other trace elements discovered. The most prevalent nitrogenous constituents were alkali-soluble protein, followed by water-soluble protein, stromal protein, salt-soluble protein, and non-protein nitrogen. Essential amino acids were abundant in pig brain (44% of total amino acids), particularly leucine (28.57 mg/g protein), threonine, valine, and lysine. The total lipid, neutral, and polar lipid fractions of the pig brain had different fatty acid compositions. The largest amount was observed in saturated fatty acids (SFA), followed by monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). Stearic acid and palmitic acid were the most common SFA. Oleic acid was the most prevalent MUFA, while docosahexaenoic acid was the most common PUFA. Thus, the pig brain can be used in food formulations as a source of nutrients.
LTBP3 Frameshift Variant in British Shorthair Cats with Complex Skeletal Dysplasia
Authors: Rudd Garces Gabriela , Knebel Anna , Hülskötter Kirsten , Jagannathan Vidhya , Störk Theresa , Hewicker-Trautwein Marion , Leeb Tosso , Volk Holger A.
Published: November 2021

We investigated a highly inbred family of British Shorthair cats in which two offspring were affected by deteriorating paraparesis due to complex skeletal malformations. Radiographs of both affected kittens revealed vertebral deformations with marked stenosis of the vertebral canal from T11 to L3. Additionally, compression of the spinal cord, cerebellar herniation, coprostasis and hypogangliosis were found. The pedigree suggested monogenic autosomal recessive inheritance of the trait. We sequenced the genome of an affected kitten and compared the data to 62 control genomes. This search yielded 55 private protein-changing variants of which only one was located in a likely functional candidate gene, LTBP3, encoding latent transforming growth factor β binding protein 3. This variant, c.158delG or p.(Gly53Alafs*16), represents a 1 bp frameshift deletion predicted to truncate 95% of the open reading frame. LTBP3 is a known key regulator of transforming growth factor β (TGF-β) and is involved in bone morphogenesis and remodeling. Genotypes at the LTBP3:c.158delG variant perfectly co-segregated with the phenotype in the investigated family. The available experimental data together with current knowledge on LTBP3 variants and their functional impact in human patients and mice suggest LTBP3:c.158delG as a candidate causative variant for the observed skeletal malformations in British Shorthair cats. To the best of our knowledge, this study represents the first report of LTBP3-related complex skeletal dysplasia in domestic animals.
Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains
Authors: Setiamarga Davin H. E. , Hirota Kazuki , Yoshida Masa-aki , Takeda Yusuke , Kito Keiji , Ishikawa Makiko , Shimizu Keisuke , Isowa Yukinobu , Ikeo Kazuho , Sasaki Takenori , Endo Kazuyoshi
Published: November 2021

Despite being a member of the shelled mollusks (Conchiferans), most members of extant cephalopods have lost their external biomineralized shells, except for the basally diverging Nautilids. Here, we report the result of our study to identify major Shell Matrix Proteins and their domains in the Nautilid Nautilus pompilius, in order to gain a general insight into the evolution of Conchiferan Shell Matrix Proteins. In order to do so, we performed a multiomics study on the shell of N. pompilius, by conducting transcriptomics of its mantle tissue and proteomics of its shell matrix. Analyses of obtained data identified 61 distinct shell-specific sequences. Of the successfully annotated 27 sequences, protein domains were predicted in 19. Comparative analysis of Nautilus sequences with four Conchiferans for which Shell Matrix Protein data were available (the pacific oyster, the pearl oyster, the limpet and the Euhadra snail) revealed that three proteins and six protein domains were conserved in all Conchiferans. Interestingly, when the terrestrial Euhadra snail was excluded, another five proteins and six protein domains were found to be shared among the four marine Conchiferans. Phylogenetic analyses indicated that most of these proteins and domains were probably present in the ancestral Conchiferan, but employed in shell formation later and independently in most clades. Even though further studies utilizing deeper sequencing techniques to obtain genome and full-length sequences, and functional analyses, must be carried out in the future, our results here provide important pieces of information for the elucidation of the evolution of Conchiferan shells at the molecular level.
Cancer as a Chronic Illness in Colombia: A Normative Consensus Approach to Improving Healthcare Services for those Living with and beyond Cancer and Its Treatment
Authors: Mendieta Cindy V. , Gómez-Neva Maria Elizabeth , Rivera-Amézquita Laura Victoria , de Vries Esther , Arévalo-Reyez Martha Lucía , Rodriguez-Ariza Santiago , Castro E Carlos J. , Faithfull Sara
Published: November 2021

Cancer survivorship care in Colombia is of increasing importance. International survivorship initiatives and studies show that continuing symptoms, psychological distress, and late effects impact the quality of life for survivors. Priorities for quality survivorship according to Colombian patients and clinicians are unknown. We undertook a nominal consensus approach with 24 participants using virtual meeting technology to identify the priorities for cancer survivorship. We applied an iterative approach conducted over eight weeks with five workshops and one patient focus group followed by a priority setting survey. The consensus group established six main themes, which were subsequently evaluated by experts: (i) symptoms and secondary effects of cancer; (ii) care coordination to increase patient access and integration of cancer care; (iii) psychosocial support after cancer treatment; (iv) mapping information resources and available support services for long-term cancer care; (v) identifying socioeconomic and regional inequalities in cancer survival to improve care and outcomes; and (vi) health promotion and encouraging lifestyle change. The order of priorities differed between clinicians and patients: patients mentioned psychosocial support as the number one priority, and clinicians prioritized symptoms and surveillance for cancer recurrence. Developing survivorship care needs consideration of both views, including barriers such as access to services and socioeconomic disparities.
Overcoming Pre-Fertilization Barriers in Intertribal Crosses between Anemone coronaria L. and Ranunculus asiaticus L.
Authors: Dhooghe Emmy , Reheul Dirk , Van Labeke Marie-Christine
Published: November 2021

Hybridization in flowering plants depends, in the first place, on the delivery of pollen to a receptive stigma and the subsequent growth of pollen tubes through the style to the ovary, where the sperm nucleus of the pollen grain can ultimately fertilize the egg cell. However, reproductive failure is often observed in distant crosses and is caused by pre- and/or post-zygotic barriers. In this study, the reproductive pre-fertilization barriers of intertribal crosses between Anemone coronaria L. and Ranunculus asiaticus L., both belonging to the Ranunculaceae, were investigated. Despite the incongruity of intertribal crosses between A. coronaria and R. asiaticus having been of low intensity at the stigmatic level, interstylar obstructions of the pollen tube growth occurred, which confirmed the presence of pre-fertilization barriers. We show that these barriers could be partially bypassed by combining pollination with a stigma treatment. More specifically, a significantly higher ratio of the pollen tube length to the total style length and a better seed set were observed when the stigma was treated with the auxin 2,4-dichlorophenoxyacetic acid (2,4-D, 1 mg·mL−1) together with the cytokinin kinetin (KIN, 0.5 mg·mL−1) 24 h after pollination, irrespective of the cross direction. More specifically, the stigma treatments with any form of auxin (combined or not combined with cytokinin) resulted in a full seed set, assuming an apomictic fruit set, because no pollination was needed to obtain these seeds.
Multiple Effects of Nitrogen Fertilization on Grape Vegetative Growth, Berry Quality and Pest Development in Mediterranean Vineyards
Authors: Cocco Arturo , Mercenaro Luca , Muscas Enrico , Mura Alessandra , Nieddu Giovanni , Lentini Andrea
Published: November 2021

Nitrogen is a key macronutrient for the quantitative and qualitative yield of grapes; in addition, it influences the development and reproduction of grape pests. The multiple effects of different nitrogen rates were investigated on the red berry cultivar ‘Carignano’ and the grape pest Planococcus ficus in a two-year field trial. Different amounts of ammonium nitrate were compared: 0, 80 and 160 Units ha−1 for mineral nitrogen. The amount of nitrogen fertilization supplied influenced the nitrogen status of vines and increased the pruning weight and leaf area, as well as the overall grape yield, by increasing the cluster weight. However, doubling the nitrogen rate did not generally increase the vegetative and productive parameters of grapevines. At harvest, nitrogen supply did not influence the anthocyanin content, tritatable acidity, and soluble solids, although the latter parameter showed a clear, yet not significant, decreasing trend. Planococcus ficus exhibited higher fecundity, survival and shorter development time on grapevines provided with nitrogen, whereas its fertility was unaffected by nitrogen fertilization. Ultimately, nitrogen had a direct and positive effect on grape yield and vine mealybug development, highlighting the importance of integrated cultural and pest control practices to promote grape production.
Sanitary Aspects of Countering the Spread of COVID-19 in Russia
Authors: Belova Elena , Shashina Ekaterina , Shcherbakov Denis , Zhernov Yury , Sukhov Vitaly , Zabroda Nadezhda , Makarova Valentina , Isiutina-Fedotkova Tatiana , Mishina Svetlana , Simanovsky Anton , Mitrokhin Oleg
Published: November 2021

Due to the conditions that cause the spread of COVID-19, national health systems worldwide are under severe strain. Most countries face similar difficulties such as a lack of medical personnel and equipment and tools for diagnosis and treatment, overrun hospitals, and forced restriction of planned medical care. Public authorities in healthcare take the following measures due to increased pressure: limiting the transmission and spread of the virus (social distancing and quarantine), mobilizing medical personnel, ensuring the availability of diagnostic and treatment tools, and providing a sufficient number of premises, which are not always suitable for the provision of medical care (buildings and structures). To date, the stages of management decision-making to counter coronavirus infection and the risk of COVID-19 transmission at various facilities have not been analyzed. The authors propose a methodology for assessing the COVID-19 transmission risk at various social and transport facilities. A survey of 1325 respondents from Moscow demonstrated the most significant risk factors, such as visitation avoidance, infection risk, and facemask wearing. Risk categories were determined and objects classified according to high, medium, and low-risk levels.
Job Satisfaction and Performance Orientation of Paramedics in German Emergency Medical Services—A Nationwide Survey
Authors: Eiche Christian , Birkholz Torsten , Konrad Fabian , Golditz Tobias , Keunecke Johann Georg , Prottengeier Johannes
Published: November 2021

(1) Background: Shortage of skilled workers is a relevant global health care problem. To remain competitive with other professions, job satisfaction is a critical issue; however, to date, there are no data available on the German EMS. This study aims to perform a statistical analysis of job satisfaction and performance orientation and to identify risk factors for low job satisfaction of paramedics in the German EMS. (2) Methods: Data were collected from 2590 paramedics through a nationwide cross-sectional survey, using the job satisfaction questionnaire by Neuberger and Allerbeck and the performance orientation questionnaire by Hippler and Krüger. Descriptive and regression statistical analysis were performed. (3) Results: The participants scored significantly lower than the reference sample on job satisfaction, with “organization and management” and “payment” being the lowest rated subscales. Around 9% of employees feared losing their jobs. While work attitude toward performance and success enhancement was high, fear of failure was also common. (4) Conclusions: Job satisfaction of paramedics in the German EMS is below that of the reference sample. Discontent with payment and organizational issues is common. Performance orientation is high, but fear of failure is frequent. Current and future efforts that aim at an attractive working environment should reflect on these findings.