bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunogenicity and Protective Activity of a Chimeric Protein Based on the Domain III of the Tick-Borne Encephalitis Virus E Protein and the OmpF Porin of Yersinia pseudotuberculosis Incorporated into the TI-Complex
Authors: Sanina Nina , Chopenko Natalia , Mazeika Andrey , Davydova Ludmila , Leonova Galina , Stenkova Anna , Uversky Vladimir N. , Kostetsky Eduard
Published: October 2018

Tick-borne encephalitis (TBE) is a widespread, dangerous infection. Unfortunately, all attempts to create safe anti-TBE subunit vaccines are still unsuccessful due to their low immunogenicity. The goal of the present work was to investigate the immunogenicity of a recombinant chimeric protein created by the fusion of the EIII protein, comprising domain III and a stem region of the tick-borne encephalitis virus (TBEV) E protein, and the OmpF porin of Yersinia pseudotuberculosis (OmpF-EIII). Adjuvanted antigen delivery systems, the tubular immunostimulating complexes (TI-complexes) based on the monogalactosyldiacylglycerol from different marine macrophytes, were used to enhance the immunogenicity of OmpF-EIII. Also, the chimeric protein incorporated into the most effective TI-complex was used to study its protective activity. The content of anti-OmpF-EIII antibodies was estimated in mice blood serum by enzyme-linked immunosorbent assay (ELISA). To study protective activity, previously immunized mice were infected with TBEV strain Dal’negorsk (GenBank ID: FJ402886). The animal survival was monitored daily for 21 days. OmpF-EIII incorporated into the TI-complexes induced about a 30–60- and 5–10-fold increase in the production of anti-OmpF-EIII and anti-EIII antibodies, respectively, in comparison with the effect of an individual OmpF-EIII. The most effective vaccine construction provided 60% protection. Despite the dramatic effect on the specific antibody titer, the studied TI-complex did not provide a statistically significant increase in the protection of OmpF-EIII protein. However, our results provide the basis of the future search for approaches to design and optimize the anti-TBEV vaccine based on the OmpF-EIII protein.
Implication and Regulation of AMPK during Physiological and Pathological Myeloid Differentiation
Authors: Jacquel Arnaud , Luciano Frederic , Robert Guillaume , Auberger Patrick
Published: October 2018

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase consisting of the arrangement of various α β, and γ isoforms that are expressed differently depending on the tissue or the cell lineage. AMPK is one of the major sensors of energy status in mammalian cells and as such plays essential roles in the regulation of cellular homeostasis, metabolism, cell growth, differentiation, apoptosis, and autophagy. AMPK is activated by two upstream kinases, the tumor suppressor liver kinase B1 (LKB1) and the calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) through phosphorylation of the kinase on Thr172, leading to its activation. In addition, AMPK inhibits the mTOR pathway through phosphorylation and activation of tuberous sclerosis protein 2 (TSC2) and causes direct activation of unc-51-like autophagy activating kinase 1 (ULK1) via phosphorylation of Ser555, thus promoting initiation of autophagy. Although it is well established that AMPK can control the differentiation of different cell lineages, including hematopoietic stem cells (HSCs), progenitors, and mature hematopoietic cells, the role of AMPK regarding myeloid cell differentiation is less documented. The differentiation of monocytes into macrophages triggered by colony stimulating factor 1 (CSF-1), a process during which both caspase activation (independently of apoptosis induction) and AMPK-dependent stimulation of autophagy are necessary, is one noticeable example of the involvement of AMPK in the physiological differentiation of myeloid cells. The present review focuses on the role of AMPK in the regulation of the physiological and pathological differentiation of myeloid cells. The mechanisms of autophagy induction by AMPK will also be addressed, as autophagy has been shown to be important for differentiation of hematopoietic cells. In addition, myeloid malignancies (myeloid leukemia or dysplasia) are characterized by profound defects in the establishment of proper differentiation programs. Reinduction of a normal differentiation process in myeloid malignancies has thus emerged as a valuable and promising therapeutic strategy. As AMPK seems to exert a key role in the differentiation of myeloid cells, notably through induction of autophagy, we will also discuss the potential to target this pathway as a pro-differentiating and anti-leukemic strategy in myeloid malignancies.
Alcohol and the Developing Brain: Why Neurons Die and How Survivors Change
Authors: Granato Alberto , Dering Benjamin
Published: October 2018

The consequences of alcohol drinking during pregnancy are dramatic and usually referred to as fetal alcohol spectrum disorders (FASD). This condition is one of the main causes of intellectual disability in Western countries. The immature fetal brain exposed to ethanol undergoes massive neuron death. However, the same mechanisms leading to cell death can also be responsible for changes of developmental plasticity. As a consequence of such a maladaptive plasticity, the functional damage to central nervous system structures is amplified and leads to permanent sequelae. Here we review the literature dealing with experimental FASD, focusing on the alterations of the cerebral cortex. We propose that the reciprocal interaction between cell death and maladaptive plasticity represents the main pathogenetic mechanism of the alcohol-induced damage to the developing brain.
Design, Synthesis, and Cytotoxic Analysis of Novel Hederagenin–Pyrazine Derivatives Based on Partial Least Squares Discriminant Analysis
Authors: Fang Kang , Zhang Xiao-Hua , Han Yao-Tian , Wu Gao-Rong , Cai De-Sheng , Xue Nan-Nan , Guo Wen-Bo , Yang Yu-Qin , Chen Meng , Zhang Xin-Yu , Wang Hui , Ma Tao , Wang Peng-Long , Lei Hai-Min
Published: October 2018

Hederagenin (He) is a novel triterpene template for the development of new antitumor compounds. In this study, 26 new He–pyrazine derivatives were synthetized in an attempt to develop potent antitumor agents; they were screened for in vitro cytotoxicity against tumor and non-tumor cell lines. The majority of these derivatives showed much stronger cytotoxic activity than He. Remarkably, the most potent was compound 9 (half maximal inhibitory concentration (IC50) was 3.45 ± 0.59 μM), which exhibited similar antitumor activities against A549 (human non-small-cell lung cancer) as the positive drug cisplatin (DDP; IC50 was 3.85 ± 0.63 μM), while it showed lower cytotoxicity on H9c2 (murine heart myoblast; IC50 was 16.69 ± 0.12 μM) cell lines. Compound 9 could induce the early apoptosis and evoke cell-cycle arrest at the synthesis (S) phase of A549 cells. Impressively, we innovatively introduced the method of cluster analysis modeled as partial least squares discriminant analysis (PLS-DA) into the structure–activity relationship (SAR) evaluation, and SAR confirmed that pyrazine had a profound effect on the antitumor activity of He. The present studies highlight the importance of pyrazine derivatives of He in the discovery and development of novel antitumor agents.
Prostaglandin E1-Mediated Collateral Recruitment Is Delayed in a Neonatal Rat Stroke Model
Authors: Bonnin Philippe , Pansiot Julien , Baud Olivier , Charriaut-Marlangue Christiane
Published: October 2018

While arterial reflow after a stroke represents an important challenge for better outcomes, it is also very important that sudden recanalization does not produce local oxidative and nitrogen species, deleterious for the brain and more particularly the immature brain. Our objective was to determine whether a supply in prostaglandin (Pg) E1 (Alprostadil), via its action on arterial pressure, might progressively improve cerebral reperfusion in a neonatal stroke model. Arterial blood flow was measured using ultrasonography. Rate-limiting and Pg terminal synthesizing enzymes were evaluated using reverse-transcriptase polymerase chain reaction. Our data suggests that a supply in PgE1 might delay and improve the ipsilateral reperfusion by decreasing thromboxane A synthase-1 gene, the density of reactive astrocytes and lesion volume.
Impact of Sarcopenia as a Prognostic Biomarker of Bladder Cancer
Authors: Fukushima Hiroshi , Takemura Kosuke , Suzuki Hiroaki , Koga Fumitaka
Published: October 2018

Sarcopenia, the degenerative and systemic loss of skeletal muscle mass, indicates patient frailty and impaired physical function. Sarcopenia can be caused by multiple factors, including advanced age, lack of exercise, poor nutritional status, inflammatory diseases, endocrine diseases, and malignancies. In patients with cancer cachexia, anorexia, poor nutrition and systemic inflammation make the metabolic state more catabolic, resulting in sarcopenia. Thus, sarcopenia is considered as one of manifestations of cancer cachexia. Recently, growing evidence has indicated the importance of sarcopenia in the management of patients with various cancers. Sarcopenia is associated with not only higher rates of treatment-related complications but also worse prognosis in cancer-bearing patients. In this article, we summarized metabolic backgrounds of cancer cachexia and sarcopenia and definitions of sarcopenia based on computed tomography (CT) images. We conducted a systematic literature review regarding the significance of sarcopenia as a prognostic biomarker of bladder cancer. We also reviewed recent studies focusing on the prognostic role of changes in skeletal muscle mass during the course of treatment in bladder cancer patients. Lastly, we discussed the impact of nutritional support, medication, and exercise on sarcopenia in cancer-bearing patients.
Highly Efficient Targeted Gene Editing in Upland Cotton Using the CRISPR/Cas9 System
Authors: Zhu Shouhong , Yu Xiuli , Li Yanjun , Sun Yuqiang , Zhu Qianhao , Sun Jie
Published: October 2018

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) gene editing system has been shown to be able to induce highly efficient mutagenesis in the targeted DNA of many plants, including cotton, and has become an important tool for investigation of gene function and crop improvement. Here, we developed a simple and easy to operate CRISPR/Cas9 system and demonstrated its high editing efficiency in cotton by targeting-ALARP, a gene encoding alanine-rich protein that is preferentially expressed in cotton fibers. Based on sequence analysis of the target site in the 10 transgenic cottons containing CRISPR/Cas9, we found that the mutation frequencies of GhALARP-A and GhALARP-D target sites were 71.4–100% and 92.9–100%, respectively. The most common editing event was deletion, but deletion together with large insertion was also observed. Mosaic mutation editing events were detected in most transgenic plants. No off-target mutation event was detected in any the 15 predicted sites analyzed. This study provided mutants for further study of the function of GhALARP in cotton fiber development. Our results further demonstrated the feasibility of use of CRISPR/Cas9 as a targeted mutagenesis tool in cotton, and provided an efficient tool for targeted mutagenesis and functional genomics in cotton.
Rice Routes of Countering Xanthomonas oryzae
Authors: Ji Zhiyuan , Wang Chunlian , Zhao Kaijun
Published: October 2018

Bacterial blight (BB) and bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, respectively, are two devastating diseases in rice planting areas worldwide. It has been proven that adoption of rice resistance is the most effective, economic, and environment-friendly strategy to avoid yield loss caused by BB and BLS. As a model system for plant—pathogen interaction, the rice—X. oryzae pathosystem has been intensively investigated in the past decade. Abundant studies have shown that the resistance and susceptibility of rice to X. oryzae is determined by molecular interactions between rice genes or their products and various pathogen effectors. In this review, we briefly overviewed the literature regarding the diverse interactions, focusing on recent advances in uncovering mechanisms of rice resistance and X. oryzae virulence. Our analysis and discussions will not only be helpful for getting a better understanding of coevolution of the rice innate immunity and X. oryzae virulence, but it will also provide new insights for application of plant R genes in crop breeding.
Effects of 1-Methyltryptophan on Immune Responses and the Kynurenine Pathway after Lipopolysaccharide Challenge in Pigs
Authors: Wirthgen Elisa , Otten Winfried , Tuchscherer Margret , Tuchscherer Armin , Domanska Grazyna , Brenmoehl Julia , Günther Juliane , Ohde Daniela , Weitschies Werner , Seidlitz Anne , Scheuch Eberhard , Kanitz Ellen
Published: October 2018

An enhanced indoleamine 2,3-dioxygenase 1 (IDO1) activity is associated with an increased mortality risk in sepsis patients. Thus, the preventive inhibition of IDO1 activity may be a promising strategy to attenuate the severity of septic shock. 1-methyltryptophan (1-MT) is currently in the interest of research due to its potential inhibitory effects on IDO1 and immunomodulatory properties. The present study aims to investigate the protective and immunomodulatory effects of 1-methyltryptophan against endotoxin-induced shock in a porcine in vivo model. Effects of 1-MT were determined on lipopolysaccharide (LPS)-induced tryptophan (TRP) degradation, immune response and sickness behaviour. 1-MT increased TRP and its metabolite kynurenic acid (KYNA) in plasma and tissues, suppressed the LPS-induced maturation of neutrophils and increased inactivity of the animals. 1-MT did not inhibit the LPS-induced degradation of TRP to kynurenine (KYN)—a marker for IDO1 activity—although the increase in KYNA indicates that degradation to one branch of the KYN pathway is facilitated. In conclusion, our findings provide no evidence for IDO1 inhibition but reveal the side effects of 1-MT that may result from the proven interference of KYNA and 1-MT with aryl hydrocarbon receptor signalling. These effects should be considered for therapeutic applications of 1-MT.
Genome-Wide Association Study Reveals Novel Genomic Regions for Grain Yield and Yield-Related Traits in Drought-Stressed Synthetic Hexaploid Wheat
Authors: Bhatta Madhav , Morgounov Alexey , Belamkar Vikas , Baenziger P. Stephen
Published: October 2018

Synthetic hexaploid wheat (SHW; 2n = 6x = 42, AABBDD, Triticum aestivum L.) is produced from an interspecific cross between durum wheat (2n = 4x = 28, AABB, T. turgidum L.) and goat grass (2n = 2x = 14, DD, Aegilops tauschii Coss.) and is reported to have significant novel alleles-controlling biotic and abiotic stresses resistance. A genome-wide association study (GWAS) was conducted to unravel these loci [marker–trait associations (MTAs)] using 35,648 genotyping-by-sequencing-derived single nucleotide polymorphisms in 123 SHWs. We identified 90 novel MTAs (45, 11, and 34 on the A, B, and D genomes, respectively) and haplotype blocks associated with grain yield and yield-related traits including root traits under drought stress. The phenotypic variance explained by the MTAs ranged from 1.1% to 32.3%. Most of the MTAs (120 out of 194) identified were found in genes, and of these 45 MTAs were in genes annotated as having a potential role in drought stress. This result provides further evidence for the reliability of MTAs identified. The large number of MTAs (53) identified especially on the D-genome demonstrate the potential of SHWs for elucidating the genetic architecture of complex traits and provide an opportunity for further improvement of wheat under rapidly changing climatic conditions.
Properties and Applications of Metal (M) dodecahydro-closo-dodecaborates (Mn=1,2B12H12) and Their Implications for Reversible Hydrogen Storage in the Borohydrides
Authors: Grahame Aiden , Aguey-Zinsou Kondo-François
Published: October 2018

Hydrogen has long been proposed as a versatile energy carrier that could facilitate a sustainable energy future. For an energy economy centred around hydrogen to function, a storage method is required that is optimised for both portable and stationary applications and is compatible with existing hydrogen technologies. Storage by chemisorption in borohydride species emerges as a promising option because of the advantages of solid-state storage and the unmatched hydrogen energy densities that borohydrides attain. One of the most nuanced challenges limiting the feasibility of borohydride hydrogen storage is the irreversibility of their hydrogen storage reactions. This irreversibility has been partially attributed to the formation of stable dodecahydro-closo-dodecaborates (Mn=1,2B12H12) during the desorption of hydrogen. These dodecaborates have an interesting set of properties that are problematic in the context of borohydride decomposition but suggest a variety of useful applications when considered independently. In this review, dodecaborates are explored within the borohydride thermolysis system and beyond to present a holistic discussion of the most important roles of the dodecaborates in modern chemistry.
Phase-Contrast and Dark-Field Imaging
Authors: Zabler Simon
Published: October 2018

Very early, in 1896, Wilhelm Conrad Röntgen, the founding father of X-rays, attempted to measure diffraction and refraction by this new kind of radiation, in vain. Only 70 years later, these effects were measured by Ulrich Bonse and Michael Hart who used them to make full-field images of biological specimen, coining the term phase-contrast imaging. Yet, another 30 years passed until the Talbot effect was rediscovered for X-radiation, giving rise to a micrograting based interferometer, replacing the Bonse–Hart interferometer, which relied on a set of four Laue-crystals for beam splitting and interference. By merging the Lau-interferometer with this Talbot-interferometer, another ten years later, measuring X-ray refraction and X-ray scattering full-field and in cm-sized objects (as Röntgen had attempted 110 years earlier) became feasible in every X-ray laboratory around the world. Today, now that another twelve years have passed and we are approaching the 125th jubilee of Röntgen’s discovery, neither Laue-crystals nor microgratings are a necessity for sensing refraction and scattering by X-rays. Cardboard, steel wool, and sandpaper are sufficient for extracting these contrasts from transmission images, using the latest image reconstruction algorithms. This advancement and the ever rising number of applications for phase-contrast and dark-field imaging prove to what degree our understanding of imaging physics as well as signal processing have advanced since the advent of X-ray physics, in particular during the past two decades. The discovery of the electron, as well as the development of electron imaging technology, has accompanied X-ray physics closely along its path, both modalities exploring the applications of new dark-field contrast mechanisms these days. Materials science, life science, archeology, non-destructive testing, and medicine are the key faculties which have already integrated these new imaging devices, using their contrast mechanisms in full. This special issue “Phase-Contrast and Dark-field Imaging” gives us a broad yet very to-the-point glimpse of research and development which are currently taking place in this very active field. We find reviews, applications reports, and methodological papers of very high quality from various groups, most of which operate X-ray scanners which comprise these new imaging modalities.
Towards Neuromorphic Learning Machines Using Emerging Memory Devices with Brain-Like Energy Efficiency
Authors: Saxena Vishal , Wu Xinyu , Srivastava Ira , Zhu Kehan
Published: October 2018

The ongoing revolution in Deep Learning is redefining the nature of computing that is driven by the increasing amount of pattern classification and cognitive tasks. Specialized digital hardware for deep learning still holds its predominance due to the flexibility offered by the software implementation and maturity of algorithms. However, it is being increasingly desired that cognitive computing occurs at the edge, i.e., on hand-held devices that are energy constrained, which is energy prohibitive when employing digital von Neumann architectures. Recent explorations in digital neuromorphic hardware have shown promise, but offer low neurosynaptic density needed for scaling to applications such as intelligent cognitive assistants (ICA). Large-scale integration of nanoscale emerging memory devices with Complementary Metal Oxide Semiconductor (CMOS) mixed-signal integrated circuits can herald a new generation of Neuromorphic computers that will transcend the von Neumann bottleneck for cognitive computing tasks. Such hybrid Neuromorphic System-on-a-chip (NeuSoC) architectures promise machine learning capability at chip-scale form factor, and several orders of magnitude improvement in energy efficiency. Practical demonstration of such architectures has been limited as performance of emerging memory devices falls short of the expected behavior from the idealized memristor-based analog synapses, or weights, and novel machine learning algorithms are needed to take advantage of the device behavior. In this article, we review the challenges involved and present a pathway to realize large-scale mixed-signal NeuSoCs, from device arrays and circuits to spike-based deep learning algorithms with ‘brain-like’ energy-efficiency.
Beta-Glucanemia after Coronary Artery Bypass Graft Surgery: A Case Report
Authors: Styczynski Ashley , Bonilla Hector , Treynor Elizabeth , Shashank Jolly , Zhang Yonglong , Finkelman Malcolm
Published: October 2018

Blood salvage techniques are increasingly being used during surgical procedures to reduce the need for exogenous blood products. The blood recovered from the surgical field through aspiration or absorption by surgical sponges is reinfused into a patient. A 65-year old patient who underwent coronary artery bypass grafting using blood salvage techniques developed a fever on post-op day 3 and was noted to have an elevated β-d-glucan level, a marker of systemic fungal infections. Ultimately, no fungal infection was identified, β-d-glucan levels slowly decreased and the patient demonstrated clinical improvement. To determine whether blood salvage procedures led to his elevated β-d-glucan levels, the surgical sponges were tested for elutable levels of β-d-glucan. The β-d-glucan content of the eluents was measured using the Fungitell® IVD kit (Associates of Cape Cod, Inc.; East Falmouth, MA). The β-d-glucan levels were found to be in concentrations 10,000-times greater than the limit of detection for human serum. While various studies have demonstrated both the immunomodulatory and pro-inflammatory effects of β-d-glucan, the physiologic impact of such high levels of β-d-glucan post-operatively remains unknown. Additionally, the persistence of detectable β-d-glucan up to several weeks after surgical procedures presents a challenge for the diagnosis of invasive fungal infections. Further studies are needed to assess the beta-glucanemia-related safety of surgical materials and their potential biological effects.
6-[1-Acetyl-5-(4-methoxyphenyl)-4,5-dihydro-1H-pyrazole-3-yl]-2(3H)-benzoxazolone
Authors: Ivanova Yordanka , Todorova Antonya , Chanev Christo , Petrov Ognyan
Published: October 2018

The title compound, 6-[1-acetyl-5-(4-methoxyphenyl)-4,5-dihydro-1H-pyrazol-3-yl]-2(3H)-benzoxazolone, was synthesized by condensation of 6-[3-(4-methoxyphenyl)-2-propenoyl]-2(3H)-benzoxazolone (1) and hydrazine hydrate in acetic acid in 84% yield. The structure of the target compound was confirmed using 1H-NMR, 13C-NMR, IR, MS, and elemental analysis.
Activation of a Raw Clay by Mechanochemical Process—Effects of Various Parameters on the Process Efficiency and Cementitious Properties
Authors: Tole Ilda , Habermehl-Cwirzen Karin , Rajczakowska Magdalena , Cwirzen Andrzej
Published: October 2018

The efficiency of the mechanochemical activation (MCA) is influenced by various process parameters as well as by the properties of the treated material. The main objective of this research was to optimize the MCA process, gaining enhancement of the chemical reactivity of a Swedish raw clay, which is going to be used as an alkali-activated cementitious binder. The effects of the amount of water, the filling ratio, the rotation speed, and the grinding duration on the amorphization degree were evaluated by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). Generally, wet and dry processes showed an extensive amorphization of both kaolinite and muscovite minerals present in the studied clay. On the contrary, quartz was amorphized mainly by the wet grinding process. The efficiency of both dry and wet grinding processes was enhanced by the increased number of grinding media versus the amount of the activated material. However, longer processing times caused significant agglomeration while a higher rotational speed enhanced the amorphization. Preliminary tests have shown that alkali activation of the processed clays produced hardened samples. Furthermore, the increased amorphization corresponded to the increased compressive strength values.
Local Deformation and Texture of Cold-Rolled AA6061 Aluminum Alloy
Authors: Zhou Diaoyu , Du Wenwen , Wen Xiyu , Qiao Junwei , Liang Wei , Yang Fuqian
Published: October 2018

Using cold rolling, we plastically deform AA6061 sheets at room temperature and investigate the variations of the microstructures, textures and local deformation of the cold-rolled AA6061 sheets as functions of thickness reduction (Δt/t0, t0 and t are the thicknesses of the AA6061 sheet before and after the cold rolling, respectively). The volume fraction of total deformation texture is relatively independent of the thickness reduction for Δt/t0 ≤ 30%, and becomes an approximately linearly increasing function of the thickness reduction for Δt/t0 > 30%. Increasing the thickness reduction causes the increase of the Vickers hardness of the cross-section of the cold-rolled sheets, which exhibits a similar increase trend to the volume fraction of total deformation texture for Δt/t0 > 30%. A simple relation between the Vickers hardness and the thickness reduction is established and is used to curve-fit the experimental results.
Stable Logic Operation of Fiber-Based Single-Walled Carbon Nanotube Transistor Circuits Toward Thread-Like CMOS Circuitry
Authors: Heo Jae , Kim Kyung-Tae , Ban Seok-Gyu , Kim Yoon-Jeong , Kim Daesik , Kim Taehoon , Hong Yongtaek , Kim In-Soo , Park Sung
Published: October 2018

A fiber-based single-walled carbon nanotube (SWCNT) thin-film-transistor (TFT) has been proposed. We designed complementary SWCNT TFT circuit based on SPICE simulations, with device parameters extracted from the fabricated fiber-based SWCNT TFTs, such as threshold voltage, contact resistance, and off-/gate-leakage current. We fabricated the SWCNTs CMOS inverter circuits using the selective passivation and n-doping processes on a fiber substrate. By comparing the simulation and experimental results, we could enhance the circuit’s performance by tuning the threshold voltage between p-type and n-type TFTs, reducing the source/drain contact resistance and off current level, and maintaining a low output capacitance of the TFTs. Importantly, it was found that the voltage gain, output swing range, and frequency response of the fiber-based inverter circuits can be dramatically improved.
Preparation and Enrichment Properties of Magnetic Dodecyl Chitosan/Silica Composite for Emerging Bisphenol Contaminants
Authors: Hu Jingrong , Liu Wangwei , Liu Huiling , Wu Lamei , Zhang Huijuan
Published: October 2018

Magnetic dodecyl chitosan/silica composite was synthesized and characterized for the enrichment of emerging bisphenol contaminants. The result demonstrated that bispehnol A, bisphenol AF, bisphenol F, and bisphenol S can be efficiently extracted via the resulting composite from aqueous solution. The adsorption rate of four investigated bisphenols on the resulting composite was achieved in the range of 87–99% within 15 min. Bispehnol A was taken as a representative adsorbate to investigate the adsorption studies in detail. The hydrophobic interaction was proposed as the principal mechanism for the adsorption of BPs. The satisfactory reusability of the resulting composite can be quickly achieved by magnetic separation technologies. Magnetic dodecyl chitosan/silica composite has a potential to be applied as a type of efficient and easily recyclable sorbent for the removal of trace organic pollutants from aqueous sample.
2D Material Science: Defect Engineering by Particle Irradiation
Authors: Schleberger Marika , Kotakoski Jani
Published: October 2018

Two-dimensional (2D) materials are at the heart of many novel devices due to their unique and often superior properties. For simplicity, 2D materials are often assumed to exist in their text-book form, i.e., as an ideal solid with no imperfections. However, defects are ubiquitous in macroscopic samples and play an important – if not imperative – role for the performance of any device. Thus, many independent studies have targeted the artificial introduction of defects into 2D materials by particle irradiation. In our view it would be beneficial to develop general defect engineering strategies for 2D materials based on a thorough understanding of the defect creation mechanisms, which may significantly vary from the ones relevant for 3D materials. This paper reviews the state-of-the-art in defect engineering of 2D materials by electron and ion irradiation with a clear focus on defect creation on the atomic scale and by individual impacts. Whenever possible we compile reported experimental data alongside corresponding theoretical studies. We show that, on the one hand, defect engineering by particle irradiation covers a wide range of defect types that can be fabricated with great precision in the most commonly investigated 2D materials. On the other hand, gaining a complete understanding still remains a challenge, that can be met by combining advanced theoretical methods and improved experimental set-ups, both of which only now begin to emerge. In conjunction with novel 2D materials, this challenge promises attractive future opportunities for researchers in this field.
A Hybrid Textile Electrode for Electrocardiogram (ECG) Measurement and Motion Tracking
Authors: An Xiang , Stylios George K.
Published: October 2018

Wearable sensors have great potential uses in personal health monitoring systems, in which textile-based electrodes are particularly useful because they are comfortable to wear and are skin and environmentally friendly. In this paper, a hybrid textile electrode for electrocardiogram (ECG) measurement and motion tracking was introduced. The hybrid textile electrode consists of two parts: A textile electrode for ECG monitoring, and a motion sensor for patient activity tracking. In designing the textile electrodes, their performance in ECG measurement was investigated. Two main influencing factors on the skin-electrode impedance of the electrodes were found: Textile material properties, and electrode sizes. The optimum textile electrode was silver plated, made of a high stitch density weft knitted conductive fabric and its size was 20 mm × 40 mm. A flexible motion sensor circuit was designed and integrated within the textile electrode. Systematic measurements were performed, and results have shown that the hybrid textile electrode is capable of recording ECG and motion signals synchronously, and is suitable for ambulatory ECG measurement and motion tracking applications.
Biodegradation of Some Organic Materials in Soils and Soil Constructions: Experiments, Modeling and Prevention
Authors: Smagin Andrey V. , Sadovnikova Nadezhda B. , Vasenev Vyacheslav I. , Smagina Marina V.
Published: October 2018

The decomposition of natural and synthetic polymeric materials (peat, humates, biochar, strongly swelling hydrogels and other soil conditioners) in a biologically and chemically active soil environment inevitably leads to a reduced ability to improve the structure, water-retention, absorptive capacity and fertility of artificial soil constructions in urbanized ecosystems and agro landscapes (constructozems). Quantitative assessment of the biodegradation process using field and laboratory incubation experiments, as well as mathematical modeling, showed the possibility of significant (up to 30–50% per year) losses of organic matter of constructozems and a corresponding deterioration of soil quality. Incubation experiments that track the carbon dioxide emission rates of polymeric materials under given thermodynamic conditions allow for the estimation of decomposition rates in addition to an exploration on the dependence of such rates on additions of microbial inhibitors. The use of nomographs provide an opportunity to optimize long-term amendment performance in soil constructions by identifying the most favorable depths to apply amendments to ensure stable functioning during desired in-service timelines in the built environment. The results of the study are useful for geo-engineers and landscaping practitioners.
Analysis of the Bearing Capacity of Helical Pile with Hexagonal Joints
Authors: Kim Daehyeon , Baek Kyemoon , Park Kyungho
Published: October 2018

This study aims to improve shafts with hexagonal joints so that they will not require welding or bolts in static load tests. In order to evaluate the bearing capacity of helical piles, two sites were selected to conduct pile installation for the field test and the pile load test. For the pile load test, a static pile load test and a dynamic pile load test were carried out, and torque was measured during pile installation in a field test to compare and analyze the expected bearing capacity and thus assess the feasibility of the method for estimating the bearing capacity. The field pile load test revealed that the bearing capacity of the gravity grout pile was the same or greater than 600 kN in the static pile load test in accordance with the AC 358 code. The non-grout pile showed a bearing capacity that was the same or smaller than 600 kN, suggesting that gravity grouting is required. Moreover, the field pile load test was used to establish the bearing capacity equation considering the torque in the pile installation, and a small number of samples were used to establish the equation, which can be used as basic data.
Gas Path Fault and Degradation Modelling in Twin-Shaft Gas Turbines
Authors: Cruz-Manzo Samuel , Panov Vili , Zhang Yu
Published: October 2018

In this study, an assessment of degradation and failure modes in the gas-path components of twin-shaft industrial gas turbines (IGTs) has been carried out through a model-based analysis. Measurements from twin-shaft IGTs operated in the field and denoting reduction in engine performance attributed to compressor fouling conditions, hot-end blade turbine damage, and failure in the variable stator guide vane (VSGV) mechanism of the compressor have been considered for the analysis. The measurements were compared with simulated data from a thermodynamic model constructed in a Simulink environment, which predicts the physical parameters (pressure and temperature) across the different stations of the IGT. The model predicts engine health parameters, e.g., component efficiencies and flow capacities, which are not available in the engine field data. The results show that it is possible to simulate the change in physical parameters across the IGT during degradation and failure in the components by varying component efficiencies and flow capacities during IGT simulation. The results also demonstrate that the model can predict the measured field data attributed to failure in the gas-path components of twin-shaft IGTs. The estimated health parameters during degradation or failure in the gas-path components can assist the development of health-index prognostic methods for operational engine performance prediction.
Design of Delivery Valve for Hydraulic Pumps
Authors: Formato Andrea , Guida Domenico , Ianniello Domenico , Villecco Francesco , Lenza Tony , Pellegrino Arcangelo
Published: October 2018

After briefly recalling the main problems that arise in the study of globe valves for alternative pumps, a methodology has been set up in order to refine the design. The obtained method has the advantages of simplicity and independence from empirical diagrams. In summary, from the obtained equation, the suitable values of the parameters can be deduced, based on the assigned data (capacity Q0 and number of rounds n) of all the dimensions of the valve or of the valves. Depending on the parameter values, it is possible to identify the most suitable kind of valve: a single dish-shaped valve, a ring valve, a valve with several rings or a group of valves.
Customized Knowledge Discovery in Databases methodology for the Control of Assembly Systems
Authors: Storti Edoardo , Cattaneo Laura , Polenghi Adalberto , Fumagalli Luca
Published: October 2018

The advent of Industry 4.0 has brought to extremely powerful data collection possibilities. Despite this, the potential contained in databases is often partially exploited, especially focusing on the manufacturing field. There are several root causes of this paradox, but the crucial one is the absence of a well-established and standardized Industrial Big Data Analytics procedure, in particular for the application within the assembly systems. This work aims to develop a customized Knowledge Discovery in Databases (KDD) procedure for its application within the assembly department of Bosch VHIT S.p.A., active in the automotive industry. The work is focused on the data mining phase of the KDD process, where ARIMA method is used. Various applications to different lines of the assembly systems show the effectiveness of the customized KDD for the exploitation of production databases for the company, and for the spread of such a methodology to other companies too.
New Silver(I) Coordination Polymer with Fe4 Single-Molecule Magnets as Long Spacer
Authors: Rigamonti Luca , Vaccari Manuela , Roncaglia Fabrizio , Baschieri Carlo , Forni Alessandra
Published: October 2018

In continuation of our work on supramolecular architectures of single-molecule magnets (SMMs) as a promising strategy in developing their magnetic performance, in this paper we report the synthesis and single crystal X-ray structure of the centered triangular tetrairon(III) SMM, [Fe4(PhpPy)2(dpm)6], Fe4 (Hdpm = dipivaloylmethane, H3PhpPy = 2-(hydroxymethyl)-2-(4-(pyridine-4-yl)phenyl)propane-1,3-diol), and its assembly in the coordination polymer {[Fe4(PhpPy)2(dpm)6Ag](ClO4)}n, Fe4Ag, upon reaction with silver(I) perchlorate. Thanks to the presence of the pyridyl rings on the two tripodal ligands, Fe4 behaves as divergent ditopic linker, and due to the Fe4:AgClO4 1:1 ratio, Fe4Ag probably possesses a linear arrangement in which silver(I) ions are linearly coordinated by two nitrogen atoms, forming 1D chains whose positive charge is balanced by the perchlorate anions. The stabilization of such a polymeric structure can be ascribed to the long distance between the two donor nitrogen atoms (23.4 Å) and their donor power. Fe4Ag shows slow relaxation of the magnetization which follows a thermally activated process with Ueff/kB = 11.17(18) K, τ0 = 2.24(17) 10−7 s in zero field, and Ueff/kB = 14.49(5) K, τ0 = 3.88(8) 10−7 s in 1-kOe applied field, in line with what reported for tetrairon(III) SMMs acting as building blocks in polymeric structures.
An Efficient Mixed Integer Linear Programming Model for the Minimum Spanning Tree Problem
Authors: Abdelmaguid Tamer F.
Published: October 2018

Finding a minimum spanning tree in a given network is a famous combinatorial optimization problem that appears in different engineering applications. Even though this problem is solvable in polynomial time, having efficient mathematical programming models is important as they can provide insights for formulating larger models that integrate other decisions in more complex applications. In the literature, there are ten different integer and mixed integer linear programming (MILP) models for this problem. They are variants of set packing, cuts, network flow and node level formulations. In addition, this paper introduces an efficient node level MILP model. Comparisons for the eleven models are provided. First, the models are compared in terms of the number of decision variables and the number of constraints. Then, computational comparisons using a commercial MILP solver on sets of randomly generated instances of different sizes are conducted. Results provide evidence that the proposed MILP model is competitive in terms of the computational time needed for proving optimality of generated solutions for instances with up to 50 nodes. Meanwhile, the LP relaxation of a multi-commodity flow MILP model which has integer polyhedron provides stable computational times despite its larger size.
A Surface of Section for Hydrogen in Crossed Electric and Magnetic Fields
Authors: Burke Korana , Mitchell Kevin
Published: October 2018

A well defined global surface of section (SOS) is a necessary first step in many studies of various dynamical systems. Starting with a surface of section, one is able to more easily find periodic orbits as well as other geometric structures that govern the nonlinear dynamics of the system in question. In some cases, a global surface of section is relatively easily defined, but in other cases the definition is not trivial, and may not even exist. This is the case for the electron dynamics of a hydrogen atom in crossed electric and magnetic fields. In this paper, we demonstrate how one can define a surface of section and associated return map that may fail to be globally well defined, but for which the dynamics is well defined and continuous over a region that is sufficiently large to include the heteroclinic tangle and thus offers a sound geometric approach to studying the nonlinear dynamics.
New Types of Fc-Contractions and the Fixed-Circle Problem
Authors: Taş Nihal , Özgür Nihal Yılmaz , Mlaiki Nabil
Published: October 2018

In this paper we investigate some fixed-circle theorems using Ćirić’s technique (resp. Hardy-Rogers’ technique, Reich’s technique and Chatterjea’s technique) on a metric space. To do this, we define new types of F c -contractions such as Ćirić type, Hardy-Rogers type, Reich type and Chatterjea type. Two illustrative examples are presented to show the effectiveness of our results. Also, it is given an application of a Ćirić type F c -contraction to discontinuous self-mappings which have fixed circles.
Urban-Tissue Optimization through Evolutionary Computation
Authors: Navarro-Mateu Diego , Makki Mohammed , Cocho-Bermejo Ana
Published: October 2018

The experiments analyzed in this paper focus their research on the use of Evolutionary Computation (EC) applied to a parametrized urban tissue. Through the application of EC, it is possible to develop a design under a single model that addresses multiple conflicting objectives. The experiments presented are based on Cerdà’s master plan in Barcelona, specifically on the iconic Eixample block which is grouped into a 4 × 4 urban Superblock. The proposal aims to reach the existing high density of the city while reclaiming the block relations proposed by Cerdà’s original plan. Generating and ranking multiple individuals in a population through several generations ensures a flexible solution rather than a single “optimal” one. Final results in the Pareto front show a successful and diverse set of solutions that approximate Cerdà’s and the existing Barcelona’s Eixample states. Further analysis proposes different methodologies and considerations to choose appropriate individuals within the front depending on design requirements.
Chrysoxanthones A–C, Three New Xanthone–Chromanone Heterdimers from Sponge-Associated Penicillium chrysogenum HLS111 Treated with Histone Deacetylase Inhibitor
Authors: Zhen Xin , Gong Ting , Wen Yan-Hua , Yan Dao-Jiang , Chen Jing-Jing , Zhu Ping
Published: October 2018

By treating with histone-deacetylase inhibitor valproate sodium, three new heterdimeric tetrahydroxanthone–chromanone lactones chrysoxanthones A–C (1–3), along with 17 known compounds were isolated from a sponge-associated Penicillium chrysogenum HLS111. The planar structures of chrysoxanthones A–C were elucidated by means of spectroscopic analyses, including MS, 1D, and 2D NMR. Their absolute configurations were established by electronic circular dichroism (ECD) calculations. Chrysoxanthones A–C exhibited moderate antibacterial activities against Bacillus subtilis with minimum inhibitory concentration (MIC) values of 5–10 μg/mL.
A Novel Cold-Adapted Leucine Dehydrogenase from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. ANT178
Authors: Wang Yatong , Hou Yanhua , Wang Yifan , Zheng Lu , Xu Xianlei , Pan Kang , Li Rongqi , Wang Quanfu
Published: October 2018

l-tert-leucine and its derivatives are useful as pharmaceutical active ingredients, in which leucine dehydrogenase (LeuDH) is the key enzyme in their enzymatic conversions. In the present study, a novel cold-adapted LeuDH, psleudh, was cloned from psychrotrophic bacteria Pseudoalteromonas sp. ANT178, which was isolated from Antarctic sea-ice. Bioinformatics analysis of the gene psleudh showed that the gene was 1209 bp in length and coded for a 42.6 kDa protein containing 402 amino acids. PsLeuDH had conserved Phe binding site and NAD+ binding site, and belonged to a member of the Glu/Leu/Phe/Val dehydrogenase family. Homology modeling analysis results suggested that PsLeuDH exhibited more glycine residues, reduced proline residues, and arginine residues, which might be responsible for its catalytic efficiency at low temperature. The recombinant PsLeuDH (rPsLeuDH) was purified a major band with the high specific activity of 275.13 U/mg using a Ni-NTA affinity chromatography. The optimum temperature and pH for rPsLeuDH activity were 30 °C and pH 9.0, respectively. Importantly, rPsLeuDH retained at least 40% of its maximum activity even at 0 °C. Moreover, the activity of rPsLeuDH was the highest in the presence of 2.0 M NaCl. Substrate specificity and kinetic studies of rPsLeuDH demonstrated that l-leucine was the most suitable substrate, and the catalytic activity at low temperatures was ensured by maintaining a high kcat value. The results of the current study would provide insight into Antarctic sea-ice bacterium LeuDH, and the unique properties of rPsLeuDH make it a promising candidate as a biocatalyst in medical and pharmaceutical industries.
Aurantoside C Targets and Induces Apoptosis in Triple Negative Breast Cancer Cells
Authors: Shrestha Sumi , Sorolla Anabel , Fromont Jane , Blancafort Pilar , Flematti Gavin R.
Published: October 2018

Triple negative breast cancer (TNBC) is a subtype of breast cancers that currently lacks effective targeted therapy. In this study, we found that aurantoside C (C828), isolated from the marine sponge Manihinea lynbeazleyae collected from Western Australia, exhibited higher cytotoxic activities in TNBC cells compared with non-TNBC (luminal and normal-like) cells. The cytotoxic effect of C828 was associated to the accumulation of cell at S-phase, resulting in the decline of cyclin D1, cyclin E1, CDK4, and CDK6, and an increase in p21. We also found that C828 inhibited the phosphorylation of Akt/mTOR and NF-kB pathways and increased the phosphorylation of p38 MAPK and SAPK/JNK pathways, leading to apoptosis in TNBC cells. These effects of C828 were not observed in non-TNBC cells at the concentrations that were cytotoxic to TNBC cells. When compared to the cytotoxic effect with the chemotherapeutic drugs doxorubicin and cisplatin, C828 was found to be 20 times and 35 times more potent than doxorubicin and cisplatin, respectively. These results indicate that C828 could be a promising lead for developing new anticancer agents that target TNBC cells.