bluuebunny/crossref_metadata_2025 huggingface dataset:
Unraveling the dynamics of urban desirability: a multi-faceted analysis of large cities
Authors: Salmani Yasamin , Ariannezhad Amin , Madraki Golshan
Published: December 2023

In the context of rapid urbanization, the challenge of accommodating growing populations while enhancing urban desirability has become a paramount concern for city planners worldwide. This study delves into the multifaceted endeavor of understanding urban desirability index, aiming to identify and analyze the attributes that contribute to the allure of large cities. Going beyond traditional perspectives, the research employs association rule mining to uncover hidden interdependencies among urban quality of life attributes that collectively shape citizens’ satisfaction. Furthermore, the study introduces the operative concepts of 'order qualifiers' and 'order winners’, borrowed from the realm of operations management. These concepts provide a novel analytical framework for evaluating pivotal dimensions of urban desirability. Leveraging both survey data and city-level statistical metrics from prominent U.S. urban centers, this research investigates the intricate interplay between economic, social, and environmental facets, thereby illuminating their cumulative influence on the satisfaction of urban residents. Notably, the study identifies median salary and community wellbeing as essential qualifiers shaping urban desirability, while also exploring the impact of nationwide excellence as a determinant of municipal desirability. These discernments serve to empower local governing bodies and policymakers in devising effective strategies for urban development and enhancement.
The impact of lowering the study design significance threshold to 0.005 on sample size in randomized cancer clinical trials
Authors: Leung Tiffany H. , Ho James C. , Wang Xiaofei , Lam Wendy W. , Pang Herbert H.
Published: December 2023

The proposal of improving reproducibility by lowering the significance threshold to 0.005 has been discussed, but the impact on conducting clinical trials has yet to be examined from a study design perspective. The impact on sample size and study duration was investigated using design setups from 125 phase II studies published between 2015 and 2022. The impact was assessed using percent increase in sample size and additional years of accrual with the medians being 110.97% higher and 2.65 years longer respectively. The results indicated that this proposal causes additional financial burdens that reduce the efficiency of conducting clinical trials.
Writing a Colonial Legal History of Northern Nigeria: An Analysis of Methods and Sources
Authors: Owolade Femi
Published: December 2023

This article analyzes the methods and sources of writing a colonial legal history of Africa. The analysis is carried out with a case study of the dual legal system operative in colonial Northern Nigeria from 1900 to 1960, which saw the English common law coexist with Islamic law. I examine how three sources of colonial law – namely, legislations, case law, and legal writings – reveal the varied perspectives of European colonial officials and Africans on the workings of this legal system. I argue that while colonial legislations and legal writings are lopsided toward the perspectives of the British authority, case law in conjunction with African commentaries provide some prospect to engage in a narrative that foregrounds the voices of Africans.
Motion of a disk embedded in a nearly inviscid Langmuir film. Part 1. Translation
Authors: Yariv Ehud , Brandão Rodolfo , Siegel Michael , Stone Howard A.
Published: December 2023

The motion of a disk in a Langmuir film bounding a liquid substrate is a classical hydrodynamic problem, dating back to Saffman (J. Fluid Mech., vol. 73, 1976, p. 593) who focused upon the singular problem of translation at large Boussinesq number,

${\textit {Bq}}\gg 1$

. A semianalytic solution of the dual integral equations governing the flow at arbitrary

${\textit {Bq}}$

was devised by Hughes et al. (J. Fluid Mech., vol. 110, 1981, p. 349). When degenerated to the inviscid-film limit

${\textit {Bq}}\to 0$

, it produces the value

$8$

for the dimensionless translational drag, which is

$50\,\%$

larger than the classical

$16/3$

-value corresponding to a free surface. While that enhancement has been attributed to surface incompressibility, the mathematical reasoning underlying the anomaly has never been fully elucidated. Here we address the inviscid limit

${\textit {Bq}}\to 0$

from the outset, revealing a singular mechanism where half of the drag is contributed by the surface pressure. We proceed beyond that limit, considering a nearly inviscid film. A naïve attempt to calculate the drag correction using the reciprocal theorem fails due to an edge singularity of the leading-order flow. We identify the formation of a boundary layer about the edge of the disk, where the flow is primarily in the azimuthal direction with surface and substrate stresses being asymptotically comparable. Utilising the reciprocal theorem in a fluid domain tailored to the asymptotic topology of the problem produces the drag correction

$(8\,{\textit {Bq}}/{\rm \pi} ) [ \ln (2/{\textit {Bq}}) + \gamma _E+1]$

,

$\gamma _E$

being the Euler–Mascheroni constant.
Science diplomacy and Asian states: Transforming the governance landscape in the Arctic
Authors: Argüello Gabriela , Rafaly Vonintsoa
Published: December 2023

As ice recedes, the governance of the Arctic is undergoing a significant change. What was once considered a frozen desert with little relevance to the legal system, the Arctic has gradually become a global object of governance. Furthermore, the growing political salience of the Arctic Ocean has generated interest in its governance beyond Arctic states, particularly Asian states such as China, India, Japan, Singapore and South Korea. These countries have been actively participating in regional cooperation arrangements, including the Arctic Council. Undoubtedly, science diplomacy has been an important driver in shaping the governance of the Arctic and maintaining it as a low-tension area. However, this perception is now being put to the test following the Russian invasion of Ukraine. Amidst this crisis, we explore whether science diplomacy can continue to promote peaceful collaboration in the Arctic region. Our research suggests that science diplomacy could potentially aid in the future of Arctic governance, particularly with regard to the involvement of Asian states. We analyse the legal and geopolitical factors involved in determining the potential roles of Asian states in Arctic governance, including whether they could serve as a bridge between the West and Russia or if their actions might further fragment Arctic governance.
Gendered Work, Skill, and Women's Labor Activism in Romanian Tobacco Factories from the 1920s to the 1960s
Authors: Ghiț Alexandra
Published: December 2023

In this article, I choose struggles over skill development as an entry point to uncovering features of women's labor activism in state-owned tobacco factories in Romania, from the 1920s to the early 1960s. I look at the processes that constructed women tobacco workers, especially those at the Tobacco Manufactory in the city of Cluj, as non-skilled workers, and examine the forms of labor activism in the tobacco industry that challenged those constructs. I describe how women's work at the Cluj Tobacco Manufactory, from the mid-1920s to the mid-1950s, was shaped by successive waves of production intensification and rationalization, demonstrating that these reorganizations affected female workers more than they affected their male coworkers. I point out that although they were considered non-skilled laborers, female tobacco workers exercised an amount of control over their work and were important contributors to their families’ maintenance. I show that spanning two different political regimes, matters of skill were at the core of labor activism. For female workers, in the interwar period, labor activism in male-dominated organizations and structures entailed skill-mediated political strategies that emphasized experience and shopfloor status besides skill. By the 1950s, labor activism encompassed engaging in confrontational politics over seasoned women workers’ lack of access to skill training programs. I show that both in the late 1920s and in the early 1950s, illiteracy and women's more limited access to formal schooling in general shaped new experiences of participation in labor politics.
George Montandon, the Ainu and the theory of hologenesis
Authors: Hennessey John L.
Published: December 2023

Argument

In 1909, Italian zoologist Daniele Rosa (1857–1944) proposed a radical new evolutionary theory: hologenesis, or simultaneous, pan-terrestrial creation and evolution driven primarily by internal factors. Hologenesis was widely ignored or rejected outside Italy, but Swiss-French anthropologist George Montandon (1879–1944) eagerly embraced and developed the theory. An ambitious careerist, Montandon’s deep investment in an obscure and unpopular theory is puzzling. Today, Montandon is best known for his virulent antisemitism and active collaboration with the Nazi occupation of France at the end of his career. By that point, however, he had quietly moved away from hologenesis. This shift has gone unnoticed or been left unexplained in existing research. This article reexamines Montandon’s theoretical outlook and reasons for championing Rosa’s forgotten theory. It argues that while Montandon’s adoption of hologenesis arose from a complex blend of scientific and personal factors, his previously overlooked early fieldwork with the Ainu played a key role. In contrast, hologenesis did not inform Montandon’s later public antisemitism.
Chickenpox Outbreaks in Three Refugee Camps on Mainland Greece, 2016-2017: A Retrospective Study
Authors: Scales Sarah Elizabeth , Park Jee Won , Nixon Rebecca , Guha-Sapir Debarati , Horney Jennifer A.
Published: December 2023

Introduction:

Displaced populations face disproportionately high risk of communicable disease outbreaks given the strains of travel, health care circumstances in their country of origin, and limited access to health care in receiving countries.

Study Objective:

Understanding the role of demographic characteristics in outbreaks is important for timely and efficient control measures. Accordingly, this study assesses chickenpox outbreaks in three large refugee camps on mainland Greece from 2016 – 2017, using clinical line-list data from Médecins du Monde (MdM) clinics.

Methods:

Clinical line-list data from MdM clinics operating in Elliniko, Malakasa, and Raidestos camps in mainland Greece were used to characterize chickenpox outbreaks in these camps. Logistic regression was used to compare the odds of chickenpox by sex, camp, and yearly increase in age. Incidences were calculated for age categories and for sex for each camp outbreak.

Results:

Across camps, the median age was 19 years (IQR: 7.00 - 30.00 years) for all individuals and five years (IQR: 2.00 - 8.00 years) for cases. Males were 55.94% of the total population and 51.32% of all cases. There were four outbreaks of chickenpox across Elliniko (n = 1), Malakasa (n = 2), and Raidestos (n = 1) camps. The odds of chickenpox when controlling for age and sex was lower for Malakasa (OR = 0.46; 95% CI, 0.38 - 0.78) and Raidestos (OR = 0.36; 95% CI, 0.24 - 0.56) when compared Elliniko. Odds of chickenpox were comparable between Malakasa and Raidestos (OR = 1.49; 95% CI, 0.92 - 2.42). Across all camps, the highest incidence was among children zero-to-five years of age. The sex-specific incidence chickenpox was higher for males than females in Elliniko and Malakasa, while the incidence was higher among females in Raidestos.

Conclusion:

As expected, individuals five years of age and under made up the majority of chickenpox cases. However, 12% of cases were teenagers or older, highlighting the need to consider atypical age groups in vaccination strategies and control measures. To support both host and displaced populations, it is important to consider risk-reduction needs for both groups. Including host communities in vaccination campaigns and activities can help reduce the population burden of disease for both communities.
Statistical Analysis of the Horizontal Phase Velocity Distribution of Atmospheric Gravity Waves and Medium‐Scale Traveling Ionospheric Disturbances in Airglow Images Over Sata (31.0°N, 130.7°E), Japan
Authors: Tsuboi Takuma , Shiokawa Kazuo , Otsuka Yuichi , Fujinami Hatsuki , Nakamura Takuji
Published: December 2023

Atmospheric gravity waves (AGWs) and medium‐scale traveling ionospheric disturbances (MSTIDs) in the upper atmosphere affect atmospheric circulation and radio‐wave transmission including satellite positioning and communications, and high‐frequency radio. The 3‐dimensional (3‐D) spectral analysis of airglow images makes it possible to extract wave parameters of AGWs and MSTIDs. Such spectral analysis of long‐term airglow images has not been performed in the southern part of Japan, which is a unique transition latitude from middle to equatorial region. In this study, we calculated the horizontal phase velocity distribution of AGWs in the mesosphere‐lower‐thermosphere (MLT), and MSTIDs in the F‐region ionosphere by applying the 3‐D spectral analysis method to the 557.7 and 630.0‐nm airglow images obtained at Sata (31.0°N, 130.7°E) in Japan over 20 years from 2000 to 2020. The spectra of AGWs in the MLT region in the 557.7‐nm airglow images show that the poleward (northward) power spectral density (PSD) is stronger in summer, suggesting that the tropospheric convection is a possible source of AGWs and the wave ducting propagation occurred. Westward propagation is also observed in winter, suggesting that wind filtering effect by mesospheric jets plays an important role. The PSD of MSTIDs in the 630‐nm airglow images increases during low solar activity periods, suggesting that MSTIDs are produced by ionospheric plasma instability. The westward PSD of MSTIDs is stronger in summer. These results are an important achievement in clarifying the statistical characteristics of AGWs and MSTIDs over Sata, Japan, based on long‐term airglow observations and 3‐D spectral analysis.
The Link Between Individual Velocities in the Incident Plasma Flow and Ion Populations Upstream of Collisionless Shocks
Authors: Wilkinson W. P. , Humphreys M. , Kilgallon S.
Published: December 2023

The interaction of the solar wind with Earth’s bow shock gives rise to several distinct ion populations upstream and downstream of the shock. Using numerical computation of trajectories in the de Hoffmann‐Teller frame of reference and previous analytical results, we investigate whether membership of these populations is determined by a particle’s incident velocity in the solar wind rest frame. The shock is assumed to be a planar surface acting as an electrostatic potential jump. Ions with insufficient energy to overcome this potential are assumed to reflect specularly. We find that ions escaping upstream come from the wings of the incident distribution, assumed to be Maxwellian, and have incoming velocities in the plasma rest frame that point upstream close to the magnetic field direction. Ions escaping after several reflections have more field‐aligned incident velocities in the upstream frame on average than those escaping after one reflection. Different ion populations occupy separate regions of the incident distribution.
Multi‐Instruments Observation of Ionospheric‐Thermospheric Dynamic Coupling Over Mohe (53.5°N, 122.3°E) During the April 2023 Geomagnetic Storm
Authors: Li Wenbo , Liu Libo , Chen Yiding , Yang Yuyan , Han Tingwei , Ding Feng , Le Huijun , Zhang Ruilong
Published: December 2023

The state and variations of the Ionosphere‐Thermosphere (I‐T) system are strongly influenced by dynamical processes, with these effects being amplified during intense space environment disturbances such as geomagnetic storms, producing multi‐scale disturbance features in the I‐T system. The redistribution of energy and momentum through the dynamical processes is currently not well understood. Unfortunately, the dearth of thermospheric observations has yielded limited studies capable of monitoring and analyzing the I‐T dynamical coupling during storms using co‐located measurements. This work examines the responses of the I‐T system during the April 2023 geomagnetic storm based on high‐resolution continuous I‐T system observations at Mohe observatory, a middle latitude station. Both large‐scale features and short‐period oscillations were identified in multiple key parameters of the I‐T system. Similar disturbed patterns in thermospheric winds occurred during the main and recovery phases of the geomagnetic storm. However, the oscillation features in many parameters of the I‐T system are only observed during the main phase. The results of concurrent I‐T system observations unveil the oscillation features of thermospheric winds, temperatures, the ionospheric F2 peak height, electron density, and total electron content. These findings reveal the important influence of wind divergence on thermospheric temperature disturbances and its modulatory effect on ionospheric disturbances.
Deep Learning‐Based Seasonal Forecast of Sea Ice Considering Atmospheric Conditions
Authors: Zhu Yilin , Qin Mengjiao , Dai Panxi , Wu Sensen , Fu Zhiyi , Chen Zhende , Zhang Laifu , Wang Yuanyuan , Du Zhenhong
Published: December 2023

The ongoing decline of sea ice in the Arctic has heightened the need for accurate sea‐ice forecasts to support environmental protection and resource development in the region and beyond. While deep learning has shown promise in seasonal sea‐ice forecasting, most of the existing models overlook the crucial influence of atmospheric factors, thereby limiting their ability to capture the intricate characteristics of the sea‐ice system and improve forecast accuracy. To address this deficiency, we propose an attention convolutional long short‐term memory ensemble network named Atsicn, which integrates atmospheric factors to enhance the precision of multi‐step seasonal sea‐ice concentration forecasts. Our findings reveal that Atsicn outperforms state‐of‐the‐art dynamic and statistical models, and demonstrates remarkable reliability in extreme years. Furthermore, the impact of atmospheric factors on sea‐ice forecasts exhibits significant seasonality, with a relatively minimal impact on forecasts from March to June, a growing impact from July to October, and a persistent yet diminishing impact from November to February. This study provides a practical approach for seasonal sea‐ice forecasts and contributes a new perspective to the understanding of the intricate interplay between sea ice and atmospheric factors.
Bdnf-Nrf-2 crosstalk and emotional behavior are disrupted in a sex-dependent fashion in adolescent mice exposed to maternal stress or maternal obesity
Authors: Musillo Chiara , Creutzberg Kerstin C. , Collacchi Barbara , Ajmone-Cat Maria Antonietta , De Simone Roberta , Lepre Marcello , Amrein Irmgard , Riva Marco A. , Berry Alessandra , Cirulli Francesca
Published: December 2023

Maternal obesity has been recognized as a stressor affecting the developing fetal brain, leading to long-term negative outcomes comparable to those resulting from maternal psychological stress, although the mechanisms have not been completely elucidated. In this study, we tested the hypothesis that adverse prenatal conditions as diverse as maternal stress and maternal obesity might affect emotional regulation and stress response in the offspring through common pathways, with a main focus on oxidative stress and neuroplasticity. We contrasted and compared adolescent male and female offspring in two mouse models of maternal psychophysical stress (restraint during pregnancy - PNS) and maternal obesity (high-fat diet before and during gestation - mHFD) by combining behavioral assays, evaluation of the hypothalamic–pituitary–adrenal (HPA) axis reactivity, immunohistochemistry and gene expression analysis of selected markers of neuronal function and neuroinflammation in the hippocampus, a key region involved in stress appraisal. Prenatal administration of the antioxidant N-acetyl-cysteine (NAC) was used as a strategy to protect fetal neurodevelopment from the negative effects of PNS and mHFD. Our findings show that these two stressors produce overlapping effects, reducing brain anti-oxidant defenses (Nrf-2) and leading to sex-dependent impairments of hippocampal Bdnf expression and alterations of the emotional behavior and HPA axis functionality. Prenatal NAC administration, by restoring the redox balance, was able to exert long-term protective effects on brain development, suggesting that the modulation of redox pathways might be an effective strategy to target common shared mechanisms between different adverse prenatal conditions.
Addressing the disparities: the approach to the African American patient with multiple myeloma
Authors: Bhutani Manisha , Blue Brandon J. , Cole Craig , Badros Ashraf Z. , Usmani Saad Z. , Nooka Ajay K. , Bernal-Mizrachi Leon , Mikhael Joseph
Published: December 2023

There are significant disparities with regards to incidence, timely diagnosis, access to treatment, clinical trial participation and health care utilization that negatively impact outcomes for African American patients with multiple myeloma. Health care providers have a role in ameliorating these disparities with thoughtful consideration of historical, sociocultural, individual and disease characteristics that influence the care provided to African American patient population. This review by a group of experts committed to health disparity in multiple myeloma provides a snapshot of disparities at both biologic and non-biologic levels, barriers to clinical care, and best practices to ensure that African American patients receive the best care available.
Functional-metabolic coupling in distinct renal cell types coordinates organ-wide physiology and delays premature ageing
Authors: Holcombe Jack , Weavers Helen
Published: December 2023

Precise coupling between cellular physiology and metabolism is emerging as a vital relationship underpinning tissue health and longevity. Nevertheless, functional-metabolic coupling within heterogenous microenvironments in vivo remains poorly understood due to tissue complexity and metabolic plasticity. Here, we establish the Drosophila renal system as a paradigm for linking mechanistic analysis of metabolism, at single-cell resolution, to organ-wide physiology. Kidneys are amongst the most energetically-demanding organs, yet exactly how individual cell types fine-tune metabolism to meet their diverse, unique physiologies over the life-course remains unclear. Integrating live-imaging of metabolite and organelle dynamics with spatio-temporal genetic perturbation within intact functional tissue, we uncover distinct cellular metabolic signatures essential to support renal physiology and healthy ageing. Cell type-specific programming of glucose handling, PPP-mediated glutathione regeneration and FA β-oxidation via dynamic lipid-peroxisomal networks, downstream of differential ERR receptor activity, precisely match cellular energetic demands whilst limiting damage and premature senescence; however, their dramatic dysregulation may underlie age-related renal dysfunction.
Magnaporthe oryzae effector MoSPAB1 directly activates rice Bsr-d1 expression to facilitate pathogenesis
Authors: Zhu Ziwei , Xiong Jun , Shi Hao , Liu Yuchen , Yin Junjie , He Kaiwei , Zhou Tianyu , Xu Liting , Zhu Xiaobo , Lu Xiang , Tang Yongyan , Song Li , Hou Qingqing , Xiong Qing , Wang Long , Ye Daihua , Qi Tuo , Zou Lijuan , Li Guobang , Sun Changhui , Wu Zhiyue , Li Peili , Liu Jiali , Bi Yu , Yang Yihua , Jiang Chunxian , Fan Jing , Gong Guoshu , He Min , Wang Jing , Chen Xuewei , Li Weitao
Published: December 2023

Fungal pathogens typically use secreted effector proteins to suppress host immune activators to facilitate invasion. However, there is rarely evidence supporting the idea that fungal secretory proteins contribute to pathogenesis by transactivating host genes that suppress defense. We previously found that pathogen Magnaporthe oryzae induces rice Bsr-d1 to facilitate infection and hypothesized that a fungal effector mediates this induction. Here, we report that MoSPAB1 secreted by M. oryzae directly binds to the Bsr-d1 promoter to induce its expression, facilitating pathogenesis. Amino acids 103-123 of MoSPAB1 are required for its binding to the Bsr-d1 promoter. Both MoSPAB1 and rice MYBS1 compete for binding to the Bsr-d1 promoter to regulate Bsr-d1 expression. Furthermore, MoSPAB1 homologues are highly conserved among fungi. In particular, Colletotrichum fructicola CfSPAB1 and Colletotrichum sublineola CsSPAB1 activate kiwifruit AcBsr-d1 and sorghum SbBsr-d1 respectively, to facilitate pathogenesis. Taken together, our findings reveal a conserved module that may be widely utilized by fungi to enhance pathogenesis.
Acetylation is required for full activation of the NLRP3 inflammasome
Authors: Zhang Yening , Luo Ling , Xu Xueming , Wu Jianfeng , Wang Fupeng , Lu Yanyan , Zhang Ningjie , Ding Yingying , Lu Ben , Zhao Kai
Published: December 2023

Full activation of the NLRP3 inflammasome needs two sequential signals: a priming signal, followed by a second, assembly signal. Several studies have shown that the two signals trigger post-translational modification (PTM) of NLRP3, affecting activity of the inflammasome, however, the PTMs induced by the second signal are less well characterized. Here, we show that the assembly signal involves acetylation of NLRP3 at lysine 24, which is important for the oligomerization and the actual assembly of NLRP3 without affecting its recruitment to dispersed trans-Golgi network (dTGN). Accordingly, NLRP3 inflammasome activation is impaired in NLRP3-K24R knock-in mice. We identify KAT5 as an acetyltransferase able to acetylate NLRP3. KAT5 deficiency in myeloid cells and pharmacological inhibition of KAT5 enzymatic activity reduce activation of the NLRP3 inflammasome, both in vitro and in vivo. Thus, our study reveals a key mechanism for the oligomerization and full activation of NLRP3 and lays down the proof of principle for therapeutic targeting of the KAT5-NLRP3 axis.
Autophagy of OTUD5 destabilizes GPX4 to confer ferroptosis-dependent kidney injury
Authors: Chu Li-Kai , Cao Xu , Wan Lin , Diao Qiang , Zhu Yu , Kan Yu , Ye Li-Li , Mao Yi-Ming , Dong Xing-Qiang , Xiong Qian-Wei , Fu Ming-Cui , Zhang Ting , Zhou Hui-Ting , Cai Shi-Zhong , Ma Zhou-Rui , Hsu Ssu-Wei , Wu Reen , Chen Ching-Hsien , Yan Xiang-Ming , Liu Jun
Published: December 2023

Ferroptosis is an iron-dependent programmed cell death associated with severe kidney diseases, linked to decreased glutathione peroxidase 4 (GPX4). However, the spatial distribution of renal GPX4-mediated ferroptosis and the molecular events causing GPX4 reduction during ischemia-reperfusion (I/R) remain largely unknown. Using spatial transcriptomics, we identify that GPX4 is situated at the interface of the inner cortex and outer medulla, a hyperactive ferroptosis site post-I/R injury. We further discover OTU deubiquitinase 5 (OTUD5) as a GPX4-binding protein that confers ferroptosis resistance by stabilizing GPX4. During I/R, ferroptosis is induced by mTORC1-mediated autophagy, causing OTUD5 degradation and subsequent GPX4 decay. Functionally, OTUD5 deletion intensifies renal tubular cell ferroptosis and exacerbates acute kidney injury, while AAV-mediated OTUD5 delivery mitigates ferroptosis and promotes renal function recovery from I/R injury. Overall, this study highlights a new autophagy-dependent ferroptosis module: hypoxia/ischemia-induced OTUD5 autophagy triggers GPX4 degradation, offering a potential therapeutic avenue for I/R-related kidney diseases.
Dilution of specialist pathogens drives productivity benefits from diversity in plant mixtures
Authors: Wang Guangzhou , Burrill Haley M. , Podzikowski Laura Y. , Eppinga Maarten B. , Zhang Fusuo , Zhang Junling , Schultz Peggy A. , Bever James D.
Published: December 2023

Productivity benefits from diversity can arise when compatible pathogen hosts are buffered by unrelated neighbors, diluting pathogen impacts. However, the generality of pathogen dilution has been controversial and rarely tested within biodiversity manipulations. Here, we test whether soil pathogen dilution generates diversity- productivity relationships using a field biodiversity-manipulation experiment, greenhouse assays, and feedback modeling. We find that the accumulation of specialist pathogens in monocultures decreases host plant yields and that pathogen dilution predicts plant productivity gains derived from diversity. Pathogen specialization predicts the strength of the negative feedback between plant species in greenhouse assays. These feedbacks significantly predict the overyielding measured in the field the following year. This relationship strengthens when accounting for the expected dilution of pathogens in mixtures. Using a feedback model, we corroborate that pathogen dilution drives overyielding. Combined empirical and theoretical evidence indicate that specialist pathogen dilution generates overyielding and suggests that the risk of losing productivity benefits from diversity may be highest where environmental change decouples plant-microbe interactions.
An inorganic-rich but LiF-free interphase for fast charging and long cycle life lithium metal batteries
Authors: Rahman Muhammad Mominur , Tan Sha , Yang Yang , Zhong Hui , Ghose Sanjit , Waluyo Iradwikanari , Hunt Adrian , Ma Lu , Yang Xiao-Qing , Hu Enyuan
Published: December 2023

Li metal batteries using Li metal as negative electrode and LiNi1-x-yMnxCoyO2 as positive electrode represent the next generation high-energy batteries. A major challenge facing these batteries is finding electrolytes capable of forming good interphases. Conventionally, electrolyte is fluorinated to generate anion-derived LiF-rich interphases. However, their low ionic conductivities forbid fast-charging. Here, we use CsNO3 as a dual-functional additive to form stable interphases on both electrodes. Such strategy allows the use of 1,2-dimethoxyethane as the single solvent, promising superior ion transport and fast charging. LiNi1-x-yMnxCoyO2 is protected by the nitrate-derived species. On the Li metal side, large Cs+ has weak interactions with the solvent, leading to presence of anions in the solvation sheath and an anion-derived interphase. The interphase is surprisingly dominated by cesium bis(fluorosulfonyl)imide, a component not reported before. Its presence suggests that Cs+ is doing more than just electrostatic shielding as commonly believed. The interphase is free of LiF but still promises high performance as cells with high LiNi0.8Mn0.1Co0.1O2 loading (21 mg/cm2) and low N/P ratio (~2) can be cycled at 2C (~8 mA/cm2) with above 80% capacity retention after 200 cycles. These results suggest the role of LiF and Cs-containing additives need to be revisited.
Geometry of visuospatial working memory information in miniature gaze patterns
Authors: Linde-Domingo Juan , Spitzer Bernhard
Published: December 2023

Stimulus-dependent eye movements have been recognized as a potential confound in decoding visual working memory information from neural signals. Here we combined eye-tracking with representational geometry analyses to uncover the information in miniature gaze patterns while participants (n = 41) were cued to maintain visual object orientations. Although participants were discouraged from breaking fixation by means of real-time feedback, small gaze shifts (<1°) robustly encoded the to-be-maintained stimulus orientation, with evidence for encoding two sequentially presented orientations at the same time. The orientation encoding on stimulus presentation was object-specific, but it changed to a more object-independent format during cued maintenance, particularly when attention had been temporarily withdrawn from the memorandum. Finally, categorical reporting biases increased after unattended storage, with indications of biased gaze geometries already emerging during the maintenance periods before behavioural reporting. These findings disclose a wealth of information in gaze patterns during visuospatial working memory and indicate systematic changes in representational format when memory contents have been unattended.
Commensal production of a broad-spectrum and short-lived antimicrobial peptide polyene eliminates nasal Staphylococcus aureus
Authors: Torres Salazar Benjamin O. , Dema Taulant , Schilling Nadine A. , Janek Daniela , Bornikoel Jan , Berscheid Anne , Elsherbini Ahmed M. A. , Krauss Sophia , Jaag Simon J. , Lämmerhofer Michael , Li Min , Alqahtani Norah , Horsburgh Malcolm J. , Weber Tilmann , Beltrán-Beleña José Manuel , Brötz-Oesterhelt Heike , Grond Stephanie , Krismer Bernhard , Peschel Andreas
Published: December 2023

Antagonistic bacterial interactions often rely on antimicrobial bacteriocins, which attack only a narrow range of target bacteria. However, antimicrobials with broader activity may be advantageous. Here we identify an antimicrobial called epifadin, which is produced by nasal Staphylococcus epidermidis IVK83. It has an unprecedented architecture consisting of a non-ribosomally synthesized peptide, a polyketide component and a terminal modified amino acid moiety. Epifadin combines a wide antimicrobial target spectrum with a short life span of only a few hours. It is highly unstable under in vivo-like conditions, potentially as a means to limit collateral damage of bacterial mutualists. However, Staphylococcus aureus is eliminated by epifadin-producing S. epidermidis during co-cultivation in vitro and in vivo, indicating that epifadin-producing commensals could help prevent nasal S. aureus carriage. These insights into a microbiome-derived, previously unknown antimicrobial compound class suggest that limiting the half-life of an antimicrobial may help to balance its beneficial and detrimental activities.
Analysis and prediction of readmissions for heart failure in the first year after discharge with INCA score
Authors: Rubini Alessia , Vilaplana-Prieto Cristina , Vázquez-Jarén Elena , Hernández-González Miriam , Félix-Redondo Francisco Javier , Fernández-Bergés Daniel
Published: December 2023

To determine the readmissions trends and the comorbidities of patients with heart failure that most influence hospital readmission rates. Heart failure (HF) is one of the most prevalent health problems as it causes loss of quality of life and increased health-care costs. Its prevalence increases with age and is a major cause of re-hospitalisation within 30 days after discharge. INCA study had observational and ambispective design, including 4,959 patients from 2000 to 2019, with main diagnosis of HF in Extremadura (Spain). The variables examined were collected from discharge reports. To develop the readmission index, capable of discriminating the population with higher probability of re-hospitalisation, a Competing-risk model was generated. Readmission rate have increased over the period under investigation. The main predictors of readmission were: age, diabetes mellitus, presence of neoplasia, HF without previous hospitalisation, atrial fibrillation, anaemia, previous myocardial infarction, obstructive pulmonary disease (COPD) and chronic kidney disease (CKD). These variables were assigned values with balanced weights, our INCA index showed that the population with values greater than 2 for men and women were more likely to be re-admitted. Previous HF without hospital admission, CKD, and COPD appear to have the greatest effect on readmission. Our index allowed us to identify patients with different risks of readmission.
The preferences of people with amyotrophic lateral sclerosis on riluzole treatment in Europe
Authors: Ludolph Albert C. , Grandjean Harish , Reviers Evy , De Micheli Valentina , Bianchi Cosetta , Cardosi Leonardo , Russ Hermann , Silani Vincenzo
Published: December 2023

The Patient Preference Survey aims to understand unmet needs related to riluzole management in people with Amyotrophic Lateral Sclerosis (ALS) and to identify which characteristics of a new formulation could better match their preferences. The survey involved 117 people with ALS (PALS) treated with riluzole in four European countries. The dysphagic PALS were least satisfied with the riluzole tablet and oral suspension and with ease in self-administration; up to 68% of respondents postponed or missed the treatment due to swallowing difficulties and need of caregiver assistance. Overall, 51% of tablet and 53% of oral suspension users regularly crushed or mixed riluzole with beverages, respectively; PALS who always manipulated riluzole showed low satisfaction with the formulation and considered the risk of choking and pneumonia the most worrisome event. The survey evaluated the driving factors in choosing/switching the therapy: 67% of PALS declared a low risk of choking. The research finally evaluated which attributes of a new formulation would be preferred: the most relevant were ease of use (4.3/5), convenient/portable packaging (4.0/5) and oral-dissolving properties without tongue motility (3.9/5). The Patient Preference Survey suggests that patients have several unmet needs and preferences that could be addressed by a different formulation, e.g. using oral film technologies.
Analysis of oxidized glucosylceramide and its effects on altering gene expressions of inflammation induced by LPS in intestinal tract cell models
Authors: Jutanom Mirinthorn , Kato Shunji , Yamashita Shinji , Toda Masako , Kinoshita Mikio , Nakagawa Kiyotaka
Published: December 2023

Glucosylceramide (GlcCer) belongs to sphingolipids and is found naturally in plant foods and other sources that humans consume daily. Our previous studies demonstrated that GlcCer prevents inflammatory bowel disease both in vitro and in vivo, whose patients are increasing alarmingly. Although some lipids are vulnerable to oxidation which changes their structure and activities, it is unknown whether oxidative modification of GlcCer affects its activity. In this research, we oxidized GlcCer in the presence of a photosensitizer, analyzed the oxide by mass spectrometric techniques, and examined its anti-inflammatory activity in lipopolysaccharide (LPS)-treated differentiated Caco-2 cells as in vitro model of intestinal inflammation. The results showed that GlcCer is indeed oxidized, producing GlcCer hydroperoxide (GlcCerOOH) as a primary oxidation product. We also found that oxidized GlcCer preserves beneficial functions of GlcCer, suppressing inflammatory-related gene expressions. These findings suggested that GlcCerOOH may perform as an LPS recognition antagonist to discourage inflammation rather than induce inflammation.
Effective processing pipeline PACE 2.0 for enhancing chest x-ray contrast and diagnostic interpretability
Authors: Siracusano Giulio , La Corte Aurelio , Nucera Annamaria Giuseppina , Gaeta Michele , Chiappini Massimo , Finocchio Giovanni
Published: December 2023

Preprocessing is an essential task for the correct analysis of digital medical images. In particular, X-ray imaging might contain artifacts, low contrast, diffractions or intensity inhomogeneities. Recently, we have developed a procedure named PACE that is able to improve chest X-ray (CXR) images including the enforcement of clinical evaluation of pneumonia originated by COVID-19. At the clinical benchmark state of this tool, there have been found some peculiar conditions causing a reduction of details over large bright regions (as in ground-glass opacities and in pleural effusions in bedridden patients) and resulting in oversaturated areas. Here, we have significantly improved the overall performance of the original approach including the results in those specific cases by developing PACE2.0. It combines 2D image decomposition, non-local means denoising, gamma correction, and recursive algorithms to improve image quality. The tool has been evaluated using three metrics: contrast improvement index, information entropy, and effective measure of enhancement, resulting in an average increase of 35% in CII, 7.5% in ENT, 95.6% in EME and 13% in BRISQUE against original radiographies. Additionally, the enhanced images were fed to a pre-trained DenseNet-121 model for transfer learning, resulting in an increase in classification accuracy from 80 to 94% and recall from 89 to 97%, respectively. These improvements led to a potential enhancement of the interpretability of lesion detection in CXRs. PACE2.0 has the potential to become a valuable tool for clinical decision support and could help healthcare professionals detect pneumonia more accurately.
The biomarkers’ landscape of post-COVID-19 patients can suggest selective clinical interventions
Authors: Paris Debora , Palomba Letizia , Albertini Maria Cristina , Tramice Annabella , Motta Lorenzo , Giammattei Eleonora , Ambrosino Pasquale , Maniscalco Mauro , Motta Andrea
Published: December 2023

In COVID-19 clinical symptoms can persist even after negativization also in individuals who have had mild or moderate disease. We here investigated the biomarkers that define the post-COVID-19 clinical state analyzing the exhaled breath condensate (EBC) of 38 post COVID-19 patients and 38 sex and age-matched healthy controls via nuclear magnetic resonance (NMR)-based metabolomics. Predicted gene-modulated microRNAs (miRNAs) related to COVID-19 were quantified from EBC of 10 patients and 10 controls. Finally, clinical parameters from all post-COVID-19 patients were correlated with metabolomic data. Post-COVID-19 patients and controls showed different metabolic phenotype (“metabotype”). From the metabolites, by using enrichment analysis we identified miRNAs that resulted up-regulated (hsa-miR146a-5p) and down-regulated (hsa-miR-126-3p and hsa-miR-223-3p) in post-COVID-19. Taken together, our multiomics data indicate that post-COVID-19 patients before rehabilitation are characterized by persistent inflammation, dysregulation of liver, endovascular thrombotic and pulmonary processes, and physical impairment, which should be the primary clinical targets to contrast the post-acute sequelae of COVID-19.
Reproduction of Varroa destructor depends on well-timed host cell recapping and seasonal patterns
Authors: Gabel Martin , Scheiner Ricarda , Steffan-Dewenter Ingolf , Büchler Ralph
Published: December 2023

Resistance traits of honeybees (Apis mellifera) against their major parasite Varroa destructor have fascinated scientists and breeders for long. Nevertheless, the mechanisms underlying resistance are still largely unknown. The same applies to possible interactions between host behaviours, mite reproduction and seasonal differences. Two resistance traits, reproductive failure of mites and recapping of brood cells, are of particular interest. High rates of recapping at the colony level were found to correspond with low reproductive success of mites. However, the direct effect of recapping on mite reproduction is still controversial and both traits seem to be very variable in their expression. Thus, a deeper knowledge of both, the effect of recapping on mite reproduction and the seasonal differences in the expression of these traits is urgently needed. To shed light on this host-parasite interaction, we investigated recapping and mite reproduction in full-grown colonies naturally infested with V. destructor. Measurements were repeated five times per year over the course of 3 years. The reproductive success of mites as well as the recapping frequency clearly followed seasonal patterns. Thereby, reproductive failure of mites at the cell level was constantly increased in case of recapping. Interestingly, this did not apply to the occurrence of infertile mites. In line with this, recapping activity in fertile cells was most frequent in brood ages in which mite offspring would be expected. Our results suggest that mite offspring is the main target of recapping. This, in turn, leads to a significantly reduced reproductive success of the parasite.
Effects of different manufacturing techniques on the performance of planar antennas
Authors: Žemgulytė Justina , Sadauskas Modestas , Ragulis Paulius , Trusovas Romualdas , Ratautas Karolis , Simniškis Rimantas , Kancleris Žilvinas , Račiukaitis Gediminas
Published: December 2023

This study investigates antenna performance based on its manufacturing process. Two types of planar antennas are manufactured on FR-4 dielectric using three different techniques: traditional lithography, laser ablation, and the novel SSAIL (selective surface activation by laser) technique. Various characteristics, such as reflection coefficient, gain, half-power beamwidth, and surface conductivity, are measured to compare the results. These findings offer invaluable insights for choosing the most suitable antenna manufacturing technique, particularly since the SSAIL technique has not been previously compared to alternative methods in the context of antenna production. In both types of antennas, the highest gain is achieved using laser ablation, with the slot-loaded patch antenna reaching 8.5 dBi and the Yagi-Uda antenna reaching 9.76 dBi. Antennas manufactured using SSAIL technology are notable for their excellent resolution and usefulness in constructing structures on non-metallized dielectrics.
A surrogate safety analysis at sharp gore areas of diverging freeway ramps using micro simulation under congested traffic conditions
Authors: Alshehri Abdulaziz H. , Farooq Muhammad Umer , Farhan Abdul , Yosri Ahmed M. , Alanazi Fayez , Deifallah Ahmed Farouk , Okail Mohamed Ahmed
Published: December 2023

Safety at the gore areas near diverging ramps is very crucial during planning and implementation of highway safety improvement programs. Limited research has been conducted on safety at the gore areas on arterial roads. This study aims at investigating the impact of improving a sharp gore area in Lincoln, Nebraska by performing a micro simulation before-and-after study with respect to its underlying state of safety and congestion. Data on travel times and traffic volumes for peak hours are incorporated after successful calibration to find out how a geometric intervention can decrease mobility issues as well as the likelihood of crash involvement. This study has utilized VISSIM software package to run the simulation however, to perform safety analysis, Surrogate Safety Assessment Model (SSAM) is used which is developed by the Federal Highway Administration (FHWA). During the model calibration, custom driving behaviors are created to represent driving tendencies of familiar drivers. The simulation results indicated that by adding an auxiliary lane near the gore area, the mobility issues such as bottle necks, lane changing dilemmas and queue lengths are substantially decreased. However, geometric interventions such as provision of a separate lane, increasing ramp spacing, nose spacing, deceleration area and queue storage area considerably reduced the likelihood of rear-end and lane changing crashes. Surrogate safety assessment in diverging ramps, particularly for sharp gores, has not previously been studied, and this study can serve as a primary footmark for future research on ramp-gores safety.