bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolism of NK cells during viral infections
Authors: Osuna-Espinoza Kenia Y. , Rosas-Taraco Adrián G.
Published: January 2023

Cellular metabolism is essential for the correct function of immune system cells, including Natural Killer cells (NK). These cells depend on energy to carry out their effector functions, especially in the early stages of viral infection. NK cells participate in the innate immune response against viruses and tumors. Their main functions are cytotoxicity and cytokine production. Metabolic changes can impact intracellular signals, molecule production, secretion, and cell activation which is essential as the first line of immune defense. Metabolic variations in different immune cells in response to a tumor or pathogen infection have been described; however, little is known about NK cell metabolism in the context of viral infection. This review summarizes the activation-specific metabolic changes in NK cells, the immunometabolism of NK cells during early, late, and chronic antiviral responses, and the metabolic alterations in NK cells in SARS-CoV2 infection. The modulation points of these metabolic routes are also discussed to explore potential new immunotherapies against viral infections.
The imbalance in the relationship between inflammatory and regulatory cytokines during gestational toxoplasmosis can be harmful to fetuses: A systematic review
Authors: Santos Priscilla Vilela dos , Toledo Débora Nonato Miranda de , de Souza Débora Maria Soares , Menezes Tatiana Prata , Perucci Luiza Oliveira , Silva Zolder Marinho , Teixeira Daniela Caldas , Vieira Ed Wilson Rodrigues , Andrade-Neto Valter Ferreira de , Guimarães Nathalia Sernizon , Talvani André
Published: January 2023

Objective

To evaluate the available information on inflammatory and regulatory plasma mediators in pregnant women (PW) diagnosed with toxoplasmosis. Source: The PubMed, Embase, Scopus, and Lilacs databases were evaluated until October 2022. Study eligibility criteria: This review was carried out following the PRISMA and registered on the PROSPERO platform (CRD42020203951). Studies that reported inflammatory mediators in PW with toxoplasmosis were considered.

Evaluation methods

After excluding duplicate articles, two authors independently carried out the process of title and abstract exclusion, and a third resolved disagreements when necessary. The full text was evaluated to detect related articles. The extraction table was built from the following data: Author, year of publication, journal name and impact factors, country, study design, number of gestations and maternal age (years), gestational period, diagnosis of toxoplasmosis, levels of inflammatory markers, laboratory tests, and clinical significance. Methodological quality was assessed using Joanna Briggs Institute tools.

Results

Of the 1,024 studies reported, only eight were included. Of the 868 PW included in this review, 20.2% were IgM+/IgG- and 50.8% were IgM-/IgG+ to T. gondii, and 29.0% uninfected. Infected PW presented higher plasma levels ofIL-5, IL-6, IL-8, IL-17, CCL5, and IL-10. Regarding the methodological quality, four studies obtained high quality. Data from this review pointed out the maintenance of the inflammatory pattern during pregnancy with a closely related to the parasite.

Conclusion

Immune status in PW defined the course of the T. gondii infection, where the equilibrium between inflammatory and regulatory cytokines mitigated the harmful placenta and fetus effects.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD420203951.
Evaluating the repetitive mucus extraction effects on mucus biomarkers, mucous cells, and the skin-barrier status in a marine fish model
Authors: Sanahuja Ignasi , Guerreiro Pedro M. , Girons Albert , Fernandez-Alacid Laura , Ibarz Antoni
Published: January 2023

Among all the mucosal barriers, the skin and its surrounding mucus are possibly the main defensive tool against changes in the environment that can be harmful for fish. Due to the extraction of this mucus being less invasive, the study of its production and functions has attracted great interest in recent years. However, there are still many gaps concerning the sampling process as well as the possible alterations in skin integrity and mucus composition. In the current study, the effects of skin mucus extraction were determined by comparing the effects of a single extraction (single extraction group, SEG) with those of three successive extractions separated by 3 days (repetitive extractions group, REG). Intact skin histology without mucus extraction (ØEG) and both plasma and skin mucus biomarkers and antibacterial capacities were also assessed. Regarding the skin histology and skin barrier properties, both the SEG and REG did not show differences in the intact skin. Interestingly, repetitive mucus extractions seemed to activate skin mucus turnover, significantly increasing the number of small-sized mucous cells (cell area< 100 µm2) and reducing the number of large-sized mucous cells (cell area > 150 µm2). Repetitive extractions significantly decreased the amounts of soluble protein and increased cortisol secretion. These metabolites remained unaltered in the plasma, indicating different responses in the plasma and mucus. Despite changes in the mucus biomarkers, antibacterial capacity against pathogenic bacteria (Pseudomonas anguilliseptica and Vibrio anguillarum) was maintained in both the plasma and mucus irrespective of the number of mucus extractions. Overall, the mucus sampling protocol had little effect on skin integrity and mucus antibacterial properties, only modifying the amounts of soluble protein exuded and stimulating mucous cell replacement. This protocol is a feasible and minimally invasive way of studying and monitoring fish health and welfare and can be used as an alternative or a complement to plasma analysis. This methodology can be transferred to farm culture conditions and be very useful for studying threatened species in order to preserve fish welfare.
Full-length transcriptome analysis provides insights into larval shell formation in Mulinia lateralis
Authors: Guo Xiaolin , Li Xixi , Zhao Feng , Liu Danwen , Yang Zujing , Li Moli , Li Yajuan , Wei Huilan , Wang Hao , Qin Zhenkui , Zhang Zhifeng , Bao Zhenmin
Published: January 2023

Mollusca is the second largest animal phylum and represents one of the most evolutionarily successful animal groups. Mulinia lateralis, a small bivalve, is a promising model organism to facilitate studies of mollusc development. However, because of the lack of published genomic and transcriptomic resources, integrated research on the formation of larval shells in this species, which is a representative developmental process of molluscs and of great importance for larva survival, is hindered. In this study, the blastula, gastrula, trochophore larva, and D-shaped larva of M. lateralis were utilized for generating a comprehensive full-length transcriptome through Pacific BioSciences (PacBio) isoform sequencing (Iso-seq) and Illumina RNA-Seq. A total of 238,919 full-length transcripts with an average length of 3,267 bp and 121,424 annotated genes were obtained. Illumina RNA-Seq data analysis showed that 4,512, 10,637, and 17,829 differentially expressed genes (DEGs) were obtained between the two adjacent developmental stages. Functional annotation and enrichment analysis revealed the specific function of genes in shell biomineralization during different developmental stages. Twelve genes that may be involved in the formation of the larval shell of M. lateralis were identified, including insoluble shell matrix protein-encoding gene 1 (ISMP1), ISMP2, ISMP5, chitin synthase, tyrosinase, chitin-binding protein, collagen and pu14 involved in shell matrix deposition, and carbonic anhydrase, solute carrier family 4 member 8 (slc4a8), EF-hand, and a calmodulin coding gene C-2442 participated in ion transportation. In addition, calcium ion binding function, calcium signaling pathway, and endocrine and other factor-regulated calcium reabsorption pathways were significantly enriched. Weighted gene correlation network analysis (WGCNA) identified two modules related to biomineralization and larval shell formation, and slc4a8 and ring finger protein 41 (rnf41) were key hub genes that may be involved in this process. Moreover, it could be implied that the process of ion transport occurs earlier than the deposition of the shell matrix. This work provided a clear view of the transcriptome for M. lateralis and will be valuable in elucidating the mechanisms of larval shell formation as well as other developmental processes in molluscs.
Finite element, analytical, artificial neural network models for carbon fibre reinforced polymer confined concrete filled steel columns with elliptical cross sections
Authors: Isleem Haytham F. , Augustino Daudi Salezi , Mohammed Ahmed Salih , Najemalden Ahmed M. , Jagadesh P. , Qaidi Shaker , Sabri Mohanad Muayad Sabri
Published: January 2023

In the present era of architecture, different cross-sectional shapes of structural concrete elements have been utilized. However, this change in shape has a significant effect on load-carrying capacity. To restore this, the use of column confinements with elliptical sections has gained attention. This paper aim to investigate the effect of elliptical shape sections of confined concrete reinforced with Carbon Fiber Reinforced Polymer (CFRP) and steel tube on axial load-carrying capacity. This study is achieved using following tools Finite Element (FE) in Abaqus and Artificial Neural Networks (ANN) modeling. The study involved a 500-mm-high column with three sets of aspect ratios: 1.0, 1.5, and 2.0. In each aspect ratio, three different layers of CFRP were used, i.e., .167, .334, and .501-mm. Analytical results showed that with the increase in aspect ratio from 1 to 2, there is a decrease in ultimate axial load of about 23.2% on average. In addition, the combined confining pressure of steel tube and CFRP increases with a decrease in dilation angle as the number of CFRP layers increases. The failure mode for the column with a large aspect ratio is local buckling at its mid-height along the minor axis. The result showed a good correlation between FE and experimental results of ultimate stress and strains, with a mean squared error of 2.27 and .001, respectively. Moreover, ANN and analytical models showed a delightful correlation of R2 of .97 for stress models and .88 for strain models, respectively. The elliptical concrete section of the column confined with steel tubes can be adopted for a new architectural type of construction; however, with more than three aspect ratios, the wrapping of the section with CFRP jackets is highly recommended.
Severe retinal hemorrhages at various levels with a serous retinal detachment in a pediatric patient with aplastic anemia–A case report
Authors: Jiang Xiaoshuang , Shen Mengxi , Liang Licong , Rosenfeld Philip J. , Lu Fang
Published: January 2023

Background

Aplastic anemia can cause ophthalmic abnormalities in patients. Vision loss in a child with aplastic anemia due to massive retinal hemorrhages at various levels is rare.

Case presentation

A pediatric patient with aplastic anemia presented with retinal hemorrhages at multiple levels along with a serous retinal detachment in both eyes and subsequent retinal changes after pars plana vitrectomy.

Conclusion

Anemia and thrombocytopenia in aplastic anemia could cause severe retinal hemorrhages and result in retinal atrophy and retinal edema. Vitrectomy can be performed to remove vitreous hemorrhage, but risk factors for retinal atrophy and edema need further investigation.
Muscle activation patterns and muscle synergies reflect different modes of coordination during upper extremity movement
Authors: Chen Xiaoling , Dong Xiaojiao , Feng Yange , Jiao Yuntao , Yu Jian , Song Yan , Li Xinxin , Zhang Lijie , Hou Peiguo , Xie Ping
Published: January 2023

A core issue in motor control is how the central nervous system generates and selects the muscle activation patterns necessary to achieve a variety of behaviors and movements. Extensive studies have verified that it is the foundation to induce a complex movement by the modular combinations of several muscles with a synergetic relationship. However, a few studies focus on the synergetic similarity and dissimilarity among different types of movements, especially for the upper extremity movements. In this study, we introduced the non-negative matrix factorization (NMF) method to explore the muscle activation patterns and synergy structure under 6 types of movements, involving the hand open (HO), hand close (HC), wrist flexion (WF), wrist extension (WE), supination (SU), and pronation (PR). For this, we enrolled 10 healthy subjects to record the electromyography signal for NMF calculation. The results showed a highly modular similarity of the muscle synergy among subjects under the same movement. Furthermore, Spearman’s correlation analysis indicated significant similarities among HO-WE, HO-SU, and WE-SU (p< 0.001). Additionally, we also found shared synergy and special synergy in activation patterns among different movements. This study confirmed the theory of modular structure in the central nervous system, which yields a stable synergetic pattern under the same movement. Our findings on muscle synergy will be of great significance to motor control and even to clinical assessment techniques.
Analytical and functional approaches to assess the immunogenicity of gluten proteins
Authors: Mamone Gianfranco , Di Stasio Luigia , Vitale Serena , Picascia Stefania , Gianfrani Carmen
Published: January 2023

Gluten proteins are the causative agents of celiac disease (CD), a lifelong and worldwide spread food intolerance, characterized by an autoimmune enteropathy. Gluten is a complex mixture of high homologous water-insoluble proteins, characterized by a high content of glutamine and proline amino acids that confers a marked resistance to degradation by gastrointestinal proteases. As a consequence of that, large peptides are released in the gut lumen with the potential to activate inflammatory T cells, in CD predisposed individuals. To date, several strategies aimed to detoxify gluten proteins or to develop immunomodulatory drugs to recover immune tolerance to gluten are under investigation. This review overviews the state of art of both analytical and functional methods currently used to assess the immunogenicity potential of gluten proteins from different cereal sources, including native raw seed flours and complex food products, as well as drug-treated samples. The analytical design to assess the content and profile of gluten immunogenic peptides, described herein, is based on the oral-gastro-intestinal digestion (INFOGEST model) followed by extensive characterization of residual gluten peptides by proteomic and immunochemical analyses. These approaches include liquid chromatography–high-resolution mass spectrometry (LC-MS/MS) and R5/G12 competitive ELISA. Functional studies to assess the immune stimulatory capabilities of digested gluten peptides are based on gut mucosa T cells or peripheral blood cells obtained from CD volunteers after a short oral gluten challenge.
Preparation of nano-selenium from chestnut polysaccharide and characterization of its antioxidant activity
Authors: Wang Shanshan , Wu Hao , Zhang Xiaoshuang , Luo Shihong , Zhou Shuang , Fan Haiyan , Lv Chunmao
Published: January 2023

Chestnut is widely cultivated and has high nutritional value due to its richness in polysaccharides. In order to improve the antioxidant activity of chestnut polysaccharide, chestnut polysaccharide (CP) was extracted by ultrasonic-assisted water extraction and alcohol precipitation and purified by cellulose DEAE-52 exchange and Sephadex G-100 chromatography in this study. CP isolates were characterized by I2-KI reaction, three-strand helical structure analysis, infrared spectrum analysis, and nuclear magnetic resonance detection. The results showed that CP is a pyrylan sugar with triple helical structure and connected by α-glycosidic bonds, with sugar residues 1,4-α-D-Glcp, 1,6-α-D-Galp, 1,5-α-L-Araf, 1,4-α-L-Rhap, and 1,4-β-D-Glcp in the CP backbone. After purification, the branching structure, rod, and spherical structure were significantly increased, with reduced lamellar structure. The in vitro scavenging rates of CP at 10 mg·mL−1 against DPPH, hydroxyl radicals, and ABTS were 88.95, 41.38, and 48.16%, respectively. The DPPH free radical scavenging rate of purified polysaccharide fraction CP-1a was slightly enhanced, and the other rates showed a small decrease. Selenized chestnut polysaccharide (CP-Se) was prepared using nano-selenium method. The selenization method was optimized and stable Se-CP was obtained. When the concentration was 5 mg·mL−1, Se-CP had significantly higher scavenging abilities 89.81 ± 2.33, 58.50 ± 1.60, and 40.66 ± 1.91% for DPPH, hydroxyl radical, and ABTS radicals, respectively, than those of CP. The results of this study provide insight into the effects purification and selenization of chestnut polysaccharide on antioxidant activity, and also provide a theoretical basis for the development of chestnut polysaccharide for use in functional foods or health products.
Hand grip strength should be normalized by weight not height for eliminating the influence of individual differences: Findings from a cross-sectional study of 1,511 healthy undergraduates
Authors: Xu Taojin , Li Xu , Wang Dingfang , Zhang Yi , Zhang Qinghua , Yan Jianyin , Jiang Junhao , Liu Wenbin , Chen Jing
Published: January 2023

Background

Hand grip strength (HGS) is a fast, useful, and inexpensive outcome predictor of nutritional status and muscular function assessment. Numerous demographic and anthropometric factors were reported to be associated with HGS, while which one or several factors produce greater variations in HGS has not been discussed in detail. This is important for answering how should HGS be normalized for eliminating the influence of individual differences in clinical practice.

Aims

To compare the contribution of age, sex, height, weight, and forearm circumference (FCF) to variations in HGS based on a large-scale sample.

Methods

We enrolled 1,511 healthy undergraduate students aged 18–23 years. Age, weight, height, and sex were obtained. HGS was measured using a digital hand dynamometer, and FCF was measured at the point of greatest circumference using a soft ruler in both hands. Pearson’s or Spearman’s correlation coefficients were calculated with data of women and men separated and mixed for comparison. Partial correlation analysis and multivariate linear regression were used to compare the effect of variables on HGS.

Results

Analysis results confirmed the correlates of higher HGS include higher height, heavier weight, being men and dominant hand, and larger FCF. The correlation between HGS and FCF was the highest, and the bivariate correlation coefficient between weight and HGS was largerata of women and men were mixed, than that between height and HGS. When data of women and men were mixed, there were moderate correlations between HGS and height and weight (r = 0.633∼0.682). However, when data were separated, there were weak correlations (r = 0.246∼0.391). Notably, partial correlation analysis revealed no significant correlation between height and HGS after eliminating the weight effect, while the correlation between weight and HGS was still significant after eliminating the height effect. Multivariate linear regression analyses revealed sex was the most significant contributor to the variation in HGS (Beta = –0.541 and –0.527), followed by weight (Beta = 0.243 and 0.261) and height (Beta = 0.102 and 0.103).

Conclusion

HGS and FCF reference values of healthy college students were provided. Weight was more correlate with hand grip strength, at least among the healthy undergraduates.

Clinical trial registration

http://www.chictr.org.cn/showproj.aspx?proj=165914, identifier ChiCTR2200058586.
Case report: Effectiveness of sirolimus in treating partial DiGeorge Syndrome with Autoimmune Lymphoproliferative Syndrome (ALPS)-like features
Authors: Gu Hao , Mou Wenjun , Chen Zhenping , Xie Xingjuan , Yao Jiafeng , Zhang Rui , Wu Runhui , Gui Jingang
Published: January 2023

Background

DiGeorge Syndrome (DGS) is a rare disease associated with 22q11.2 chromosomal microdeletion, also known as a velocardiofacial syndrome, based on the frequent involvements of the palate, facial, and heart problems. Hematologic autoimmunity is rare in DGS but presents with a refractory course and poor prognosis. Herein, we report a case of partial DGS in a patient with refractory immune cytopenia and autoimmune lymphoproliferative syndrome (ALPS)-like manifestations.

Case description

A 10-year-old boy with growth retardation presented initially with a ventricular septal defect at 7 months old, which had been repaired soon after. The patient suffered from thrombocytopenia and progressed into chronic refractory immune thrombocytopenia (ITP) at 30 months old. One year later, the patient developed multilineage cytopenias including thrombocytopenia, neutropenia, and anemia. First-line treatment of ITP, like high-dose dexamethasone and intravenous immunoglobulin, had little or short-term effect on controlling symptoms. Whole-exome sequencing revealed the presence of a de novo heterozygous 2.520 Mb deletion on chromosome 22q11.21. Moreover, decreased proportion of naive T cells and elevated double-negative T cells were found. The patient was given sirolimus therapy (1.5 mg/m2, actual blood concentration range: 4.0–5.2 ng/ml) without adding other immunosuppressive agents. The whole blood cell count was gradually restored after a month, and the disease severity was soothed with less frequency of infections and bleeding events. Decreased spleen size and restrained lymph node expansion were achieved after 3-month sirolimus monotherapy.

Conclusions

This case is the first description on the efficacy of sirolimus monotherapy to treat refractory multilineage cytopenias of DGS presented with ALPS-like features.
Growth of a cohort of very low birth weight and preterm infants born at a single tertiary health care center in South Africa
Authors: Michaelis Isabel Alexandra , Krägeloh-Mann Ingeborg , Mazinu Mikateko , Jordaan Esme
Published: January 2023

Background

Very low birth weight (VLBW) and extremely low birth weight (ELBW) infants are known to be at high risk of growth failure and developmental delay later in life. The majority of those infants are born in low and middle income countries.

Aim

Growth monitoring in a cohort of infants born with a VLBW up to 18 months corrected age was conducted in a low resource setting tertiary hospital.

Methods

In this prospective cohort study, 173 infants with a birth weight below 1,501 g admitted within their first 24 h of life were recruited and the 115 surviving until discharged were asked to follow up at 1, 3, 6, 12 and 18 months. Weight, height and head circumferences were recorded and plotted on WHO Z-score growth charts.

Results

Of the 115 discharged infants 89 were followed up at any given follow-up point (1, 3, 6, 12 and/or 18 months). By 12 months of corrected age another 15 infants had demised (13.0%). The infants' trends in weight-for-age z-scores (WAZ) for corrected age was on average below the norm up to 12 months (average estimated z-score at 12 months = −0.44; 95% CI, −0.77 to −0.11), but had reached a normal range on average at 18 months = −0.24; 95% CI, −0.65 to 0.19) with no overall difference in WAZ scores weight between males and female' infants (p > 0.7). Similar results were seen for height at 12 months corrected age with height-for-age z-scores (HAZ) of the study subjects being within normal limits (−0.24; 95% CI, −0.63 to 0.14). The mean head circumference z-scores (HCZ) initially plotted below −1.5 standard deviations (S.D.), but after 6 months the z-scores were within normal limits (mean z-score at 7 months = −0.19; 95% CI, −0.45 to 0.06).

Conclusion

Weight gain, length and head circumferences in infants with VLBW discharged showed a catch-up growth within the first 6–18 months of corrected age, with head circumference recovering best. This confirms findings in other studies on a global scale, which may be reassuring for health systems such as those in South Africa with a high burden of children born with low birth weights.
Case report: A de novo Non-sense SOX9 mutation (p.Q417*) located in transactivation domain is Responsible for Campomelic Dysplasia
Authors: Qiao Xingxing , Wu Liping , Tang Jianjun , Xiang Rong , Fan Liangliang , Huang Hao , Chen Yaqin
Published: January 2023

Background

Campomelic dysplasia (CD) is an autosomal dominant skeletal dysplasia syndrome characterized by shortness and bowing of lower extremities, and often accompanied by XY sex reversal. Heterozygous pathogenic variants of SOX9 or rearrangement involving the long arm of chromosome 17 are the causes of disease. However, evidence for pathogenesis of SOX9 haploinsufficiency is insufficient.

Methods

We enrolled a Chinese family where the fetus was diagnosed with CD. The affected fetus was selected for whole-exome sequencing to identify the pathogenic mutations in this family.

Results

After data filtering, a novel non-sense SOX9 variant (NM_000346.3; c.1249C > T; p.Q417*) was identified as the pathogenic lesion in the fetus. Further co-segregation analysis using Sanger sequencing confirmed that this novel SOX9 mutation (c.1249C > T; p.Q417*) was a de novo mutation in the affected fetus. This terminated codon mutation identified by bioinformatics was located at an evolutionarily conserved site of SOX9. The bioinformatics-based analysis predicted this variant was pathogenic and affected SOX9 transactivation activity.

Conclusion

CD is a rare condition, which connected with SOX9 tightly. We identified a novel heterozygous SOX9 variant (p.Q417*) in a Chinese CD family. Our study supports the putative reduced transactivation of SOX9 variants in the pathogenicity of CD.
Quantum-inspired optimization for wavelength assignment
Authors: Boev Aleksey S. , Usmanov Sergey R. , Semenov Alexander M. , Ushakova Maria M. , Salahov Gleb V. , Mastiukova Alena S. , Kiktenko Evgeniy O. , Fedorov Aleksey K.
Published: January 2023

Problems related to wavelength assignment (WA) in optical communications networks involve allocating transmission wavelengths for known transmission paths between nodes that minimize a certain objective function, for example, the total number of wavelengths. Playing a central role in modern telecommunications, this problem belongs to NP-complete class for a general case so that obtaining optimal solutions for industry-relevant cases is exponentially hard. In this work, we propose and develop a quantum-inspired algorithm for solving the wavelength assignment problem. We propose an advanced embedding procedure to transform this problem into the quadratic unconstrained binary optimization (QUBO) form, having a improvement in the number of iterations with price-to-pay being a slight increase in the number of variables (“spins”). Then, we compare a quantum-inspired technique for solving the corresponding QUBO form against classical heuristic and industrial combinatorial solvers. The obtained numerical results indicate on an advantage of the quantum-inspired approach in a substantial number of test cases against the industrial combinatorial solver that works in the standard setting. Our results pave the way to the use of quantum-inspired algorithms for practical problems in telecommunications and open a perspective for further analysis of the use of quantum computing devices.
Non-destructive detection of kiwifruit soluble solid content based on hyperspectral and fluorescence spectral imaging
Authors: Xu Lijia , Chen Yanjun , Wang Xiaohui , Chen Heng , Tang Zuoliang , Shi Xiaoshi , Chen Xinyuan , Wang Yuchao , Kang Zhilang , Zou Zhiyong , Huang Peng , He Yong , Yang Ning , Zhao Yongpeng
Published: January 2023

The soluble solid content (SSC) is one of the important parameters depicting the quality, maturity and taste of fruits. This study explored hyperspectral imaging (HSI) and fluorescence spectral imaging (FSI) techniques, as well as suitable chemometric techniques to predict the SSC in kiwifruit. 90 kiwifruit samples were divided into 70 calibration sets and 20 prediction sets. The hyperspectral images of samples in the spectral range of 387 nm~1034 nm and the fluorescence spectral images in the spectral range of 400 nm~1000 nm were collected, and their regions of interest were extracted. Six spectral pre-processing techniques were used to pre-process the two spectral data, and the best pre-processing method was selected after comparing it with the predicted results. Then, five primary and three secondary feature extraction algorithms were used to extract feature variables from the pre-processed spectral data. Subsequently, three regression prediction models, i.e., the extreme learning machines (ELM), the partial least squares regression (PLSR) and the particle swarm optimization - least square support vector machine (PSO-LSSVM), were established. The prediction results were analyzed and compared further. MASS-Boss-ELM, based on fluorescence spectral imaging technique, exhibited the best prediction performance for the kiwifruit SSC, with the Rp2, Rc2 and RPD of 0.8894, 0.9429 and 2.88, respectively. MASS-Boss-PLSR based on the hyperspectral imaging technique showed a slightly lower prediction performance, with the Rp2, Rc2, and RPD of 0.8717, 0.8747, and 2.89, respectively. The outcome presents that the two spectral imaging techniques are suitable for the non-destructive prediction of fruit quality. Among them, the FSI technology illustrates better prediction, providing technical support for the non-destructive detection of intrinsic fruit quality.
Characterization of the endophytic bacterial community of Bituminaria bituminosa plant grown in vitro and its interaction with the plant extract
Authors: Chiellini Carolina , De Leo Marinella , Longo Vincenzo , Pieracci Ylenia , Pistelli Laura
Published: January 2023

Introduction

Bituminaria bituminosa is a medicinal plant recognized for its phytochemicals, such as furanocoumarins, pterocarpans, and flavonoids. Since the secondary metabolism is influenced by the plant-endophyte interactions, the endophytic bacterial community of B. bituminosa was explored and the possible interactions with the plant were described.

Materials and methods

Different bacterial strains were isolated from different organs of in vitro plants as shoots, roots, and seeds. The bacterial strains were identified and phenotypically characterized for different traits; strains were also exposed to different concentrations of B. bituminosa plant extract showing different susceptibility, probably determined by different secondary metabolites produced by the plant in the different organs (i.e. aerial parts and roots).

Results and discussion

Bacterial strains showed different phenotypic characteristics; the 6 detected haplotypes were dominated by a single species related to Stenotrophomonas rhizophila. Endophytes isolated from the aerial parts produced a higher indole-3-acetic acid (IAA) amount than those of the roots, while all strains were unable to produce biosurfactants and antagonistic activity toward the other strains. The research opens new perspectives for future analysis addressed to test the susceptibility of the endophytic bacterial community of B. bituminosa toward the pure compounds extracted from the plants, and to investigate the role of these compounds on the distribution of endophytes within the different plant tissues.
Security vs. civil liberties: How citizens cope with threat, restriction, and ideology
Authors: Jäger Felix
Published: January 2023

How do citizens balance their preferences for civil liberties and security in the context of a competitive party system? Using the case of terrorism and counter-terrorism, I argue that the willingness to support restrictions of civil liberties does not only depend on external shocks and being targeted by a counter-policy. Instead, it also depends on their ideological match with policymakers and terrorist actors. Using an original survey experiment conducted in Germany in 2022, I study how the four factors feeling threatened by a terrorist attack, being targeted by a surveillance measure, the ideology behind an attack, and the partisanship of counteracting politicians influence the attitudes of citizens and whether these factors are mutually dependent. While earlier research has focused on one kind of terrorism (mostly Islamic), this paper examines various forms of terrorism (religious, right-wing, and climate-radical) and how they affect peoples' attitudes toward civil liberties and surveillance. The results show that terrorist ideology plays a minor role, but that it matters whether citizens sympathize with the party that proposes a policy. The study extends our understanding of the political consequences of polarization, threat perceptions of terrorism, and public support for surveillance policies.
Introducing Peer-supported Open Dialogue in changing mental health care
Authors: Lorenz-Artz Karin , Bierbooms Joyce , Bongers Inge
Published: January 2023

The need to transform mental health care toward person-centered, recovery-based, and network-oriented care is recognized worldwide. Open Dialogue (OD) is seen as a hopeful approach in the context of this transformation and is introduced in countries around the globe. Five Dutch mental health care organizations spread over the Netherlands introduced the Peer-supported Open Dialogue (POD) approach, which adds an explicit role of peer-support workers to the OD approach. It appeared that (P)OD-trained professionals face issues in introducing the (P)OD approach in existing MHC settings. One of the reasons, which is the focus of this study, may be that they encounter difficulties in explaining to non-(P)OD-trained professionals what (P)OD entails. The main objective of this study is to provide guidance to and contribute to making (P)OD better understandable for non-(P)OD-trained professionals. In this study, we used a qualitative design and conducted 23 semi-structured interviews with POD-trained professionals with various backgrounds, to cultivate a rich understanding of which aspects could contribute to a better understanding of POD for non-POD-trained professionals. We used a hybrid approach to analyze the data, meaning that the technique of both inductive and deductive thematic analyses has been applied. From these analyses, six aspects emerged that could give guidance to and contribute to making (P)OD more understandable for non-(P)OD-trained professionals: (1) Experiencing (P)OD by attending treatment network sessions, (2) a coherent and profound narrative about (P)OD, (3) adjusting terminology to better fit the context, such as the two terms “principles” and “responsibility” in this study, (4) the order in which (P)OD elements are introduced in the narrative, (5) bringing the elements “presence,” “reflecting,” and “expertise by experience” more to the foreground, and (6) conceptualizing the main elements in a “talking paper.” A better understanding of (P)OD might be one of the building blocks for improving (P)OD adoption in existing MHC practices, which are on their way toward person-centered, recovery-based, and network-oriented care.
Exploring the effects of risk-taking, exploitation, and exploration on divergent thinking under group dynamics
Authors: Harada Tsutomu
Published: January 2023

This study examined the effects of risk-taking and exploitation/exploration trade-off on divergent thinking in individuals, dyads, and triads. We adopted a simple Q-learning model to estimate risk attitudes, exploitation, and exploration parameters. The results showed that risk-taking, exploitation, and exploration did not affect divergent thinking in dyads. Instead, loss aversion was negatively related to divergent thinking. In contrast, risk attitudes and the inverse temperature as a ratio between exploitation and exploration were significant but with contrasting effects in individuals and triads. For individuals, risk-taking, exploitation and loss aversion played a critical role in divergent thinking. For triads, risk aversion and exploration were significantly related to divergent thinking. However, the results also indicated that balancing risk with exploitation/exploration and loss aversion is critical in enhancing divergent thinking in individuals and triads when learning coherence emerges. These results could be interpreted consistently with related literature such as the odd-vs. even-numbered group dynamics, knowledge diversity in group creativity, and representational change theory in insight problem-solving.
Sleep – the guarantee of health! Does the environmental perception characteristics of urban residential areas affect residents' sleep quality?
Authors: Zhu Xun , Gao Ming , Cheng Xinting , Zhao Wei
Published: January 2023

A complex urban living environment and residents' sleep quality are intrinsically linked. Nonetheless, there is little evidence that the residential environment affects sleep quality. Based on the results of subjective questionnaires, this study uses the multiple regression combined with mediation analysis to construct a mechanical model of the impact of urban residential environmental perception characteristics on residents' sleep quality. Moreover, the differences among the influence intensities of the significant factors are compared and the results show that (1) in low-density environments (FAR < 2) and lower floors (4–6), residents sleep longer and have better sleep quality; (2) the environmental quality and service facilities of the physical environment and the sense of safety in the social environment have a significant impact on residents' sleep quality; and (3) the mental health of residents play a significant intermediary role in the relationship between social environment and sleep quality, with the highest effect accounting for 33.88%. The influence mechanisms of various environmental factors in a residential area on sleep quality were revealed and a more refined design basis for a healthy urban living environment, community renewal, and renovation was provided.
Does the way we do science foster discovery?
Authors: Sivadas Nithin
Published: January 2023

Freedom to explore the unknown is key to scientific discovery. Maximizing modern individualistic measures of scientific productivity like the number of citations and publications may impede the progress of science as a whole.
Unsolved problems in Strong Thermal Emission Velocity Enhancement (STEVE) and the picket fence
Authors: Nishimura Yukitoshi , Dyer Alan , Kangas Lauri , Donovan Eric , Angelopoulos Vassilis
Published: January 2023

This paper reviews key properties and major unsolved problems about Strong Thermal Emission Velocity Enhancement (STEVE) and the picket fence. We first introduce the basic characteristics of STEVE and historical observations of STEVE-like emissions, particularly the case on 11 September 1891. Then, we discuss major open questions about STEVE: 1) Why does STEVE preferentially occur in equinoxes? 2) How do the solar wind and storm/substorm conditions control STEVE? 3) Why is STEVE rare, despite that STEVE does not seem to require extreme driving conditions? 4) What are the multi-scale structures of STEVE? 5) What mechanisms determine the properties of the picket fence? 6) What are the chemistry and emission mechanisms of STEVE? 7) What are the impacts of STEVE on the ionosphere−thermosphere system? Also, 8) what is the relation between STEVE, stable auroral red (SAR) arcs, and the subauroral proton aurora? These issues largely concern how STEVE is created as a unique mode of response of the subauroral magnetosphere−ionosphere−thermosphere coupling system. STEVE, SAR arcs, and proton auroras, the three major types of subauroral emissions, require energetic particle injections to the pre-midnight inner magnetosphere and interaction with cold plasma. However, it is not understood why they occur at different times and why they can co-exist and transition from one to another. Strong electron injections into the pre-midnight sector are suggested to be important for driving intense subauroral ion drifts (SAID). A system-level understanding of how the magnetosphere creates distinct injection features, drives subauroral flows, and disturbs the thermosphere to create optical emissions is required to address the key questions about STEVE. The ionosphere−thermosphere modeling that considers the extreme velocity and heating should be conducted to answer what chemical and dynamical processes occur and how much the STEVE luminosity can be explained. Citizen scientist photographs and scientific instruments reveal the evolution of fine-scale structures of STEVE and their connection to the picket fence. Photographs also show the undulation of STEVE and the localized picket fence. High-resolution observations are required to resolve fine-scale structures of STEVE and the picket fence, and such observations are important to understand underlying processes in the ionosphere and thermosphere.
Object detection and classification using few-shot learning in smart agriculture: A scoping mini review
Authors: Ragu Nitiyaa , Teo Jason
Published: January 2023

Smart agriculture is the application of modern information and communication technologies (ICT) to agriculture, leading to what we might call a third green revolution. These include object detection and classification such as plants, leaves, weeds, fruits as well as animals and pests in the agricultural domain. Object detection, one of the most fundamental and difficult issues in computer vision has attracted a lot of attention lately. Its evolution over the previous two decades can be seen as the pinnacle of computer vision advancement. The detection of objects can be done via digital image processing. Machine learning has achieved significant advances in the field of digital image processing in current years, significantly outperforming previous techniques. One of the techniques that is popular is Few-Shot Learning (FSL). FSL is a type of meta-learning in which a learner is given practice on several related tasks during the meta-training phase to be able to generalize successfully to new but related activities with a limited number of instances during the meta-testing phase. Here, the application of FSL in smart agriculture, with particular in the detection and classification is reported. The aim is to review the state of the art of currently available FSL models, networks, classifications, and offer some insights into possible future avenues of research. It is found that FSL shows a higher accuracy of 99.48% in vegetable disease recognition on a limited dataset. It is also shown that FSL is reliable to use with very few instances and less training time.
Effect of processing and substitution levels of Ukwa (Treculia africana) on the anti-nutrient factors, in-vitro starch and protein digestibility and total essential amino-acid content of snack bars
Authors: Edima-Nyah Anne P. , Ntukidem Victor E. , Ekanem Moses C. , Ojimelukwe Philippa C. , Nwabueze Titus U.
Published: January 2023

Introduction

Ukwa (Treculia africana) is an indigenous edible seed. It is a strategic store of vital food nutrients that are available during a specific crucial time of the year when reliable sources of these nutrients are under cultivation and extremely scarce. In the past, only poor rural residents used to eat it, but today it is not only a specialty meal enjoyed by Nigeria's elite and metropolitan residents but also a source of foreign exchange.

Methods

In this study, Ukwa was processed into whole, dehulled, malted and defatted flours. Maize and coconut flours were blended with the respective Ukwa flours at the ratio of 0:95:5, 20:75:5, 25:70:5, 30:65:5, 35:60:5 and 95:0:5 for Ukwa flour:Maize flour:Coconut grits respectively for development of snack bars. A two-factor factorial experiment in a completely randomized block design was applied for the study of the effect of processing, substitution levels of Ukwa flour and the interaction of the two variables on the responses analyzed. Anti-nutrient content and proximate compositions of the flours were determined using standard procedures.

Results

Processing significantly reduced the anti-nutrient content and increased protein content of Ukwa flours. Up to 51.72% reduction of tannin was obtained by dehulling. Defatting, malting and dehulling resulted in 18.75, 34.37 and 65.62% reduction respectively in oxalate content. Highest reduction was obtained by dehulling, and was 70.69% in phytate, 79.95% in saponin, and 48.17% in trypsin inhibitor activity. Crude protein content of snack bars had 16.16 to 25.46% substitution main effect, and 19.43 to 22.65% processing main effect. In-vitro protein and starch digestibility increased with processing and decreased with increase in substituted level of Ukwa in the blend. Improvement, up to 9.97% by dehulling, 9.86% by malting and 8.64% by defatting, was recorded in in-vitro protein digestibility. An increase of 3.00 to 24.10% by defatting, 5.90 to 29.09% by malting and 9.70 to 31.80% by dehulling was recorded in in-vitro starch digestibility.

Discussion

Our study revealed that, all the processing methods adopted reduced the anti-nutrient content of ukwa flours. Protein content and total essential amino-acid (TEAA) showed significant increase with increased substituted level of ukwa flour. In-vitro protein and starch digestibility decreased with increase substituted level of ukwa flour. Snack bars formulated with 20% Ukwa showed the highest in-vitro starch and protein digestibility irrespective of the method of processing. Malted Ukwa based snack bars recorded the highest values of TEAA. Processing of this nutritious seeds and use of its flour to develop snack bars could enhance utilization and give convenience to consumers and encourage extensive farming of the crop.
Electrohydraulic lithotripsy through endoscopic retrograde cholangiopancreatography combined with SpyGlass in the treatment of complex pancreatic duct stones: A case report and literature review
Authors: Pu Weigao , Ma Chenhui , Wang Bofang , Wang Yunpeng , Wang Haiyun , Xu Bo , He Puyi , Cui Hongbin , Chen Hao
Published: January 2023

The incidence of pancreatic duct stones (PDS) is less than 1%. After the formation of stones, the lumen of the pancreatic duct is blocked, and the pancreatic juice cannot be discharged smoothly, resulting in the impairment of the internal and external secretions of the pancreas. Several national guidelines now recommend endoscopic retrograde cholangiopancreatography (ERCP) as the treatment for PDS. The emergence of SpyGlass makes it possible to visualize the ERCP blind area of the pancreatic system directly. Electrohydraulic lithotripsy (EHL) under SpyGlass can crush large and pressure-resistant stones into smaller fragments, significantly improving the success of the endoscopic treatment of large stones. Here, we report a patient presented with acute alcohol-associated pancreatitis, found to have PDS on imaging, who underwent ERCP combined with SpyGlass (EHL), avoiding surgery, reducing trauma, and being discharged from the hospital with a rapid recovery. Therefore, endoscopic therapy is effective and safe for PDS patients. The combination therapy of this patient is the first use of SpyGlass for PDS in our centre, which marks a new stage in the application of endoscopic therapy for pancreatic diseases.