Evaluation of in vitro cytotoxicity of «ACVR-IN-01» in a human mesenchymal stromal cell model
Authors:   Burnasyan State Research Center of the Federal Medical Biophysical Centre of the Federal Medical Biological Agency, Moscow, Russian Federation, Pustovoyt V.I. , Astrelina T.A. ,   Burnasyan State Research Center of the Federal Medical Biophysical Centre of the Federal Medical Biological Agency, Moscow, Russian Federation, Balakin E.I. ,   Burnasyan State Research Center of the Federal Medical Biophysical Centre of the Federal Medical Biological Agency, Moscow, Russian Federation, Kobzeva I.V. ,   Burnasyan State Research Center of the Federal Medical Biophysical Centre of the Federal Medical Biological Agency, Moscow, Russian Federation, Stepanov A.A. ,   Scientific and Practical Educational Center of the V.N.Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russian Federation, Sinitsyna A.A. ,   Scientific and Practical Educational Center of the V.N.Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russian Federation, Izotov A.A. ,   Scientific and Practical Educational Center of the V.N.Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russian Federation, Butkova T.V. ,   Scientific and Practical Educational Center of the V.N.Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russian Federation, Belorusova A.E. ,   Burnasyan State Research Center of the Federal Medical Biophysical Centre of the Federal Medical Biological Agency, Moscow, Russian Federation, Samoilov A.S. ,   Burnasyan State Research Center of the Federal Medical Biophysical Centre of the Federal Medical Biological Agency, Moscow, Russian Federation
Published: July 2023

Objective. Evaluate the cytotoxicity of a new chemical compound «ACVR-IN-01» (in vitro) on a culture of human mesenchymal stromal cells (MSCs). Materials and methods. The characterized MSC culture of the 4th passage from the biobank of cell cultures was used. Test samples «ACVR-IN-01» were prepared at concentrations of 25, 200 and 400 μg/ml. For each tested concentration of «ACVRIN-01» cultivation was carried out in triplicate. A visual assessment of the state of the cell culture was performed after 24, 48 hours and 7 days. The assessment of the functional state of MSCs was carried out by identifying the total number of viable cells and immunophenotypic studies of cell culture. Results. Cultivation of MSCs in a complete growth medium with «ACVR-IN-01» at a concentration of 25, 200 and 400 μg/ml for 7 days does not affect the expression of CD90 and CD105 antigens at all points of observation in vitro. Cultivation of MSCs in a complete growth medium with «ACVR-IN-01» at concentrations of 25, 200 and 400 μg/ml for 7 days did not cause the expression of CD34 and CD45 antigens at all in vitro observation points. Conclusion. The chemical compound «ACVR-IN-01» does not have a significant effect on the linear affiliation of MSCs. Evaluation of the cytotoxicity of the test substance «ACVR-IN-01» (400 μg/ml) on a cell culture in a complete growth medium showed a pronounced decrease in the expression of the CD73 antigen on the 7th day of the experimental in vitro study, accompanied by a loss of the differentiation potential of MSCs in the osteogenic and chondrogenic direction. Key words: herpes virus infection, cytotoxicity, mesenchymal stromal cells, «ACVR-IN-01»