bluuebunny/crossref_metadata_2025 huggingface dataset:
Raman Spectroscopic Investigation of Osteoclastic Activity under the Influence of Bisphosphonate
Authors: Marin Elia , Adachi Tetsuya , Boschetto Francesco , Zhu Wenliang , Adachi Keiji , Kanamura Narisato , Yamamoto Toshiro , Pezzotti Giuseppe
Published: April 2022

The bone resorption inhibitor bisphosphonate (BP) is used to prevent fractures in patients with osteoporosis and bone metastases caused by cancer. However, BP induces apoptosis of osteoclasts and excessively suppresses bone turnover, so that side effects such as jawbone necrosis have become a problem. In the super-aging society that Japan is facing, it is expected that jawbone necrosis (Medication-related osteonecrosis of the jaw: MRONJ) will increase as the number of osteoporosis patients increases. There are many unclear points about the pathophysiology of jawbone necrosis, and there have been attempts to clarify it. Most of the research on osteoclasts so far has comprised destructive and invasive analyses, such as TRAP staining and PCR by culturing osteoclasts on a plastic plate, which is the original physiological function of osteoclasts. “Bone resorption” cannot be analyzed in real time. In this study, Raman spectroscopy is used to show the state of bone resorption of osteoclasts cultured on ivory sections or octacalcium phosphate plates noninvasively and without the need for colorimetric assays. This makes it possible to clarify the effect of BP on osteoclast metabolism in an environment closer to that of a living body. If this method is established, then we aim to elucidate the pathophysiology of bone pathologies and medical treatments that directly affect osteoclasts, such as medication-related osteonecrosis, and establish a diagnostic method.
Comparative Evaluation of the Thermal, Structural, Chemical and Morphological Properties of Bagasse from the Leaf and Fruit of Bromelia hemisphaerica Lam. Delignified by Organosolv
Authors: Tapia-Maruri Daniel , Evangelista-Lozano Silvia , Alamilla-Beltrán Liliana , Camacho-Díaz Brenda Hildeliza , Ávila-Reyes Sandra Victoria , Villalobos-Espinosa Julieta del Carmen , Jiménez-Aparicio Antonio Ruperto
Published: April 2022

Bromelia hemisphaerica Lam., a wild plant native to Mexico, has medicinal attributes and is mainly used for its hemisphericin content in foods. However, the residues of its leaves and fruits are underutilized, representing an area of opportunity for foods or materials. Lignocellulosic material from leaves and fruit bagasse was isolated using an organosolv treatment to separate their components (cellulose, hemicellulose and lignin) and to determine the influence after processing on the physicochemical, thermal and microstructural characteristics. The extracted fiber presented a cellulose content of 44% in the leaf and 33.5% in the fruit. The release of lignin after the organosolv process represented a greater amount of amorphous material in the leaf than in the fruit. By FTIR and X-ray diffraction (DRx), the change in the crystallinity of the cellulose was determined (from 18% higher in the leaf than the fruit before to 14% higher in the fruit after the organosolv process), with values similar to type I cellulose. The thermal properties showed a high order in the structure of the cellulose. Microscopy and digital analysis techniques showed the microstructural changes and the effectiveness of delignification during the process. It is concluded that the leaf fiber of B. hemisphaerica presents characteristics that make it useful as a potential ingredient for food product development and other uses.
The Curing and Strength Properties of Highly Moist Waste Mud from Slurry Shield Tunnel Construction
Authors: Ding Ziyu , Liu Tao , Zhang Yan , Su Xiuting , Zheng Jianguo
Published: April 2022

The waste mud from slurry shield tunnel construction can easily cause environmental pollution. Thus, the RCSLP curing agent is developed to reutilize the waste mud produced in the Jinan Yellow River tunnel. The strength properties of the soils solidified with cement or RCSLP were tested, and their microstructures were studied by scanning electron microscopy. The results show that for the RCSLP curing agent, an optimum cement content of 15% and an optimum additive content of 12% improved the early compressive strength of the solidified soil. The mechanical properties of the RCSLP solidified soil were improved due to the significantly increased hydration products. The findings of this research could help achieve the utilization of waste mud from slurry shield tunnel construction.
A Numerical Aerodynamic Analysis on the Effect of Rear Underbody Diffusers on Road Cars
Authors: Guerrero Alex , Castilla Robert , Eid Giorgio
Published: April 2022

The aerodynamic complexity of the underbody surfaces of conventional road vehicles is a matter of fact. Currently available literature is focused mainly on very simple Ahmed-body geometries as opposed to realistic car shapes, due to their complexity and computational cost. We attempted to understand the flow behaviour around different realistic conventional road car geometries, and we provide an extensive evaluation of the aerodynamic loads generated. The key findings of this article could potentially set a precedent and be useful within the automotive industry’s investigations on drag-reduction mechanisms or sources of downforce generation. The novelty of the work resides in the realistic approach employed for the geometries and in the investigation of barely researched aerodynamic elements, such as front diffusers, which might pave the way for further research studies. A baseline flat-underfloor design, a 7∘ venturi diffuser-equipped setup, a venturi diffuser with diagonal skirts, and the same venturi diffuser with frontal slot-diffusers are the main configurations we studied. The numerical predictions evaluated using RANS computational fluid dynamics (CFD) simulations deal with the aerodynamic coefficients. The configuration that produced the highest downforce coefficient was the one composed of the 7∘ venturi diffuser equipped with diagonal sealing skirts, achieving a CL value of −0.887, which represents an increase of around 1780% with regard to the baseline model. That achievement and the gains in higher vertical loads also entail a compromise with an increase in the overall air resistance. The performance achieved with diffusers in the generation of downforce is, as opposed to the one obtained with conventional wings, a cleaner alternative, by avoiding wake disturbances and downwash phenomena.
Influence of Unburned Carbon on Environmental-Technical Behaviour of Coal Fly Ash Fired Clay Bricks
Authors: Dacuba Juan , Cifrian Eva , Romero Maximina , Llano Tamara , Andrés Ana
Published: April 2022

The incorporation of coal fly ash (CFA) in fired clay bricks (FCBs), as a clay replacement, contributes toward cleaner production practices. CFA disposal is an important issue worldwide due to its huge volume and to its potential negative environmental impacts, and currently does not have a recovery route due to its high concentration in unburned materials. In this study, the impact of the incorporation of two CFA, with different content of unburned carbon, FAA (low LOI) and FAB (high LOI) in FCBs, from a technical and environmental point of view was conducted. Unburned carbon plays an important role on the final properties of FCBs. The thermal decomposition during the firing process promotes an increase of water absorption, decreasing the flexural strength as the porosity increases, although the technical and mechanical properties of samples containing up to 30% FAA and percentages of 20% FAB are acceptable. The leaching behaviour showed an immobilisation of Cr and Se in FCBs while Mo reduced its mobility to values below non-hazardous limits. Acid gas emission values do not exceed the reference emission value, except for SO2 emissions while the level of CO2 emissions must be estimated based on the total annual production of the ceramic factory.
Bioactive Peptides from Lupinus spp. Seed Proteins-State-of-the-Art and Perspectives
Authors: Garmidolova Aleksandra , Desseva Ivelina , Mihaylova Dasha , Lante Anna
Published: April 2022

Nowadays, the search for food-suitable plant proteins is a great challenge. In addition to their sustainability and nutritional value, the focus is more and more on possible positive interactions with human health. To date, the presence of bioactive peptides encrypted in the structure of protein opens new perspectives, addressing the food industry’s request for new ingredients with technological properties and also the nutraceutical and pharmaceutical sectors based on multifunctional health applications. Lupinus is a sustainable genus of the legume family Fabaceae, and the lupin seed-derived bioactive peptides have demonstrated different effects including anti-inflammatory, antidiabetic, antioxidant, antibacterial, hypocholesterolemic, and antihypertensive activities. This review aims to discuss the current knowledge on lupin protein and their bioactive peptides, highlighting the documented health claims, but also the possibility of allergenicity and the work to be done for the development of new functional products.
Eco Valorization of Eucalyptus globulus Bark and Branches through Liquefaction
Authors: Fernandes Ana , Cruz-Lopes Luísa , Dulyanska Yuliya , Domingos Idalina , Ferreira José , Evtuguin Dmitry , Esteves Bruno
Published: April 2022

Eucalyptus globulus forest residues, bark, and branches, were characterized by wet chemistry methods and involved in the liquefaction process using a glycerol-ethylene glycol reaction mixture (1:1, v/v) catalyzed by strong mineral acid (3% H2SO4) or strong mineral base (6% KOH). The effect of the reaction conditions (temperature and duration) and the particle size on the yield of liquefied products have been evaluated. Acid catalysis revealed remarkably higher yields (25–50%) than when using basic catalyst. It was considered that bark was more vulnerable to liquefaction with respect to particle size than branches. Too high temperatures (>180 °C) are not advantageous regarding the liquefaction yields and, therefore, temperatures around 160–180 °C would be preferable. The best yield for the bark sample (>80 mesh fraction) was obtained at 180 °C for 60 min (61.6%), while for the branches the best yield was obtained at 160 °C for 60 min (62.2%). Under compromised conditions (180 °C for 60 min), the fine fraction (>80 mesh) of bark and branches did not show significant differences between their liquefaction yields and can be processed together while adjusting the suitable processing time. The main advantage of the use of these residues instead of solid wood is that it would bring the Forest managing companies a much higher income for their wastes that are usually burned and the use of lignocellulosic materials in detriment of petroleum-based materials for the production of polymers would make industry less dependent on oil prices fluctuations.
Effect of Catalyst Ink and Formation Process on the Multiscale Structure of Catalyst Layers in PEM Fuel Cells
Authors: Liu Huiyuan , Ney Linda , Zamel Nada , Li Xianguo
Published: April 2022

The structure of a catalyst layer (CL) significantly impacts the performance, durability, and cost of proton exchange membrane (PEM) fuel cells and is influenced by the catalyst ink and the CL formation process. However, the relationship between the composition, formulation, and preparation of catalyst ink and the CL formation process and the CL structure is still not completely understood. This review, therefore, focuses on the effect of the composition, formulation, and preparation of catalyst ink and the CL formation process on the CL structure. The CL structure depends on the microstructure and macroscopic properties of catalyst ink, which are decided by catalyst, ionomer, or solvent(s) and their ratios, addition order, and dispersion. To form a well-defined CL, the catalyst ink, substrate, coating process, and drying process need to be well understood and optimized and match each other. To understand this relationship, promote the continuous and scalable production of membrane electrode assemblies, and guarantee the consistency of the CLs produced, further efforts need to be devoted to investigating the microstructure of catalyst ink (especially the catalyst ink with high solid content), the reversibility of the aged ink, and the drying process. Furthermore, except for the certain variables studied, the other manufacturing processes and conditions also require attention to avoid inconsistent conclusions.
On the Safe Deployment of Matrix Multiplication in Massively Parallel Safety-Related Systems
Authors: Fernández Javier , Perez-Cerrolaza Jon , Agirre Irune , Calderon Alejandro J. , Abella Jaume , Cazorla Francisco J.
Published: April 2022

Deep learning technology has enabled the development of increasingly complex safety-related autonomous systems using high-performance computers, such as graphics processing units (GPUs), which provide the required high computing performance for the execution of parallel computing algorithms, such as matrix–matrix multiplications (a central computing element of deep learning software libraries). However, the safety certification of parallel computing software algorithms and GPU-based safety-related systems is a challenge to be addressed. For example, achieving the required fault-tolerance and diagnostic coverage for random hardware errors. This paper contributes with a safe matrix–matrix multiplication software implementation for GPUs with random hardware error-detection capabilities (permanent, transient) that can be used with different architectural patterns for fault-tolerance, and which serves as a foundation for the implementation of safe deep learning libraries for GPUs. The proposed contribution is complementary and can be combined with other techniques, such as algorithm-based fault tolerance. In particular, (i) we provide the high-performance matrix multiplication CUTLASS library with a catalog of diagnostic mechanisms to detect random hardware errors down to the arithmetic operation level; and (ii) we measure the performance impact incurred by the adoption of these mechanisms and their achievable diagnostic coverage with a set of representative matrix dimensions. To that end, we implement these algebraic operations, targeting CUDA cores with single instructions and multiple-thread math instructions in an NVIDIA Xavier NX GPU.
Synchrotron X-ray Radiation (SXR) in Medical Imaging: Current Status and Future Prospects
Authors: Tamal Mahbubunnabi , Althobaiti Murad , Alomari Abdul-Hakeem , Dipty Sumaiya Tabassum , Suha Khadiza Tun , Al-Hashim Maryam
Published: April 2022

Synchrotron X-ray radiation (SXR) has been widely studied to explore the structure of matter. Recently, there has been an intense focus on the medical application of SXR in imaging. This review is intended to explore the latest applications of SXR in medical imaging and to shed light on the advantages and drawbacks of this modality. The article highlights the latest developments in other fields that can greatly enhance the capability and applicability of SXR. The potentials of using machine and deep learning (DL)-based methods to generate synthetic images to use in regular clinics along with the use of photon counting X-ray detectors for spectral medical imaging with SXR are also discussed.
Observational Analyses of Dry Intrusions and Increased Ozone Concentrations in the Environment of Wildfires
Authors: Georgiev Christo G. , Tjemkes Stephen A. , Karagiannidis Athanasios , Prieto Jose , Lagouvardos Konstantinos
Published: April 2022

In this study, atmospheric dynamical processes, which govern the intensification of wildfire activity and the associated increase in low-level ozone concentrations, were studied using images, advanced products and vertical profiles derived from satellite observations. The analyses confirm that the influence of deep stratospheric intrusions, identified in the satellite water vapor imagery, on a fire-risk area contributes to the increase in fire activity. The depth of dry stratospheric intrusions, the associated synoptic evolution and the enhanced low-level ozone concentrations caused by vertical transport of stratospheric air and/or related to biomass burning emissions were analyzed using satellite measurements from SEVIRI, IASI and CrIS instruments, complemented with surface observations near the wildfires’ locations. It is shown that the spatial and vertical resolutions of these soundings provide a way of identifying areas of enhanced ozone downwind of wildfires. Influences of the upper-troposphere dynamics and the wind field evolution as factors of uncertainty and complexity in studying the ozone production from wildfire emissions are considered. The combination of satellite soundings and satellite estimations of fire radiative energy and WV imagery may contribute to better understand the ozone enhancement associated with stratospheric intrusion and wildfire emissions.
Note on the Higher-Order Derivatives of the Hyperharmonic Polynomials and the r-Stirling Polynomials of the First Kind
Authors: Cereceda José
Published: April 2022

In this paper, we focus on the higher-order derivatives of the hyperharmonic polynomials, which are a generalization of the ordinary harmonic numbers. We determine the hyperharmonic polynomials and their successive derivatives in terms of the r-Stirling polynomials of the first kind and show the relationship between the (exponential) complete Bell polynomials and the r-Stirling numbers of the first kind. Furthermore, we provide a new formula for obtaining the generalized Bernoulli polynomials by exploiting their link with the higher-order derivatives of the hyperharmonic polynomials. In addition, we obtain various identities involving the r-Stirling numbers of the first kind, the Bernoulli numbers and polynomials, the Stirling numbers of the first and second kind, and the harmonic numbers.
Strong Chromatic Index of Outerplanar Graphs
Authors: Wang Ying , Wang Yiqiao , Wang Weifan , Cui Shuyu
Published: April 2022

The strong chromatic index χs′(G) of a graph G is the minimum number of colors needed in a proper edge-coloring so that every color class induces a matching in G. It was proved In 2013, that every outerplanar graph G with Δ≥3 has χs′(G)≤3Δ−3. In this paper, we give a characterization for an outerplanar graph G to have χs′(G)=3Δ−3. We also show that if G is a bipartite outerplanar graph, then χs′(G)≤2Δ; and χs′(G)=2Δ if and only if G contains a particular subgraph.
Influence of Wall Compliance on the Flow Patterns in a Patient-Specific Brachio-Cephalic Arterio-Venous Fistula
Authors: Alam Neda , Newport David
Published: April 2022

Patients with end stage renal disease require some form of vascular access for treatment, with Arterio-Venous Fistulas (avf) being the preferred form available due to better patency rates. However, they continue to present complications after creation, leading to early or late failure. While many studies are examining the flow in patient-specific fistulas, they often neglect the influence of vessel compliance on its hemodynamics. The objective of this study is to investigate the effect of wall compliance on the complex hemodynamics of a patient-specific brachio-cephalic avf and how it differs from a rigid fistula. Particle Image Velocimetry (piv) was used to capture the flow pattern within the fistula for both steady (Re = 1817) and pulsatile (Reav=1817, Remax=2232) flow conditions. The results were compared to rigid model measurements performed under the same Reynolds number. The streamline plots and coefficient of variation results did not differ significantly between the models; however, the non-dimensional velocity and directional variability results did vary between the two fistulas. A difference of approximately 8% was seen between the two models for both steady and pulsatile flow. The findings of this study suggest that to determine the bulk flow, a rigid model is adequate, but to capture the finer details of the flow, a compliant model is necessary.
Mycomerge: Fabrication of Mycelium-Based Natural Fiber Reinforced Composites on a Rattan Framework
Authors: Nguyen Mai Thi , Solueva Daniela , Spyridonos Evgenia , Dahy Hanaa
Published: April 2022

There is an essential need for a change in the way we build our physical environment. To prevent our ecosystems from collapsing, raising awareness of already available bio-based materials is vital. Mycelium, a living fungal organism, has the potential to replace conventional materials, having the ability to act as a binding agent of various natural fibers, such as hemp, flax, or other agricultural waste products. This study aims to showcase mycelium’s load-bearing capacities when reinforced with bio-based materials and specifically natural fibers, in an alternative merging design approach. Counteracting the usual fabrication techniques, the proposed design method aims to guide mycelium’s growth on a natural rattan framework that serves as a supportive structure for the mycelium substrate and its fiber reinforcement. The rattan skeleton is integrated into the finished composite product, where both components merge, forming a fully biodegradable unit. Using digital form-finding tools, the geometry of a compressive structure is computed. The occurring multi-layer biobased component can support a load beyond 20 times its own weight. An initial physical prototype in furniture scale is realized. Further applications in architectural scale are studied and proposed.
The Impact of Music on Stress Biomarkers: Protocol of a Substudy of the Cluster-Randomized Controlled Trial Music Interventions for Dementia and Depression in ELderly Care (MIDDEL)
Authors: Rasing Naomi L. , Janus Sarah I. M. , Kreutz Gunter , Sveinsdottir Vigdis , Gold Christian , Nater Urs M. , Zuidema Sytse U.
Published: April 2022

Recently, a large cluster-randomized controlled trial was designed—Music Interventions for Dementia and Depression in ELderly care (MIDDEL)—to assess the effectiveness of music interventions on depression in care home residents with dementia (ClinicalTrials.gov NCT03496675). To understand the pathophysiological mechanisms, we observed the effect of repeated music interventions on stress in this population since chronic stress was associated with depression and an increased risk for dementia. An exploratory study was designed to assess: (1) changes in hair cortisol concentrations as an indicator of longer-term stress; (2) whether baseline stress is a predictor of therapy outcome; (3) pre- and post-treatment effects on salivary α-amylase and cortisol response as an indicator of immediate stress in 180–200 care home residents with dementia and depressive symptoms who partake in the MIDDEL trial. Insights into mediatory effects of stress to explain the effect of music interventions will be gained. Hair cortisol concentrations were assessed at baseline and at 3, 6, and 12 months along with the Perceived Stress Scale. Salivary α-amylase and cortisol concentrations were assessed at 1, 3, and 6 months. Saliva was collected just before a session and 15 and 60 min after a session, along with a stress Visual Analogue Scale.
Effects of Working from Home on Greenhouse Gas Emissions and the Associated Energy Costs in Six Australian Cities
Authors: Navaratnam Satheeskumar , Jayalath Amitha , Aye Lu
Published: April 2022

Working from home (WFH) has been imposed due to the COVID-19 pandemic. The adoption of WFH impacts energy use in the residential, commercial, and transportation sectors. Consequently, this affects the greenhouse gas emission (GHGE) and the associated energy costs to workers and employers. This study estimates the effects of WFH on the GHGE and energy-related costs in the residential, commercial, and transportation sectors. A simple linear model was used to estimate the changes in the GHGEs and cost by a typical employee when WFH practice is adopted for 1.5 and 4 days per week. The adoption of WFH reduces the operational GHGE accounted for commercial buildings and transport. However, it increases the operational GHGE accounted for residential buildings, which is a maximum of about 6% and 12%, respectively, for WFH 1.5 and 4 days. The reduction of GHGE from transport is significantly higher than that of residential buildings. The GHGE reductions from the transport sector are about 30% and 80%, respectively, for WFH 1.5 days and 4 days per week. WFH for 1.5 and 4 days per week reduces the national annual GHGE by about 1.21 Mt CO2-e and 5.76 Mt CO2-e, respectively. Further, the annual transportation cost of an employee is reduced by 30% and 80% in each city when the employee WFH for 1.5 and 4 days per week. The outcomes of this study offer a direction to reduce energy consumption and related costs and potential future research avenues on this topic. Further, the findings also help policymakers develop a hybrid work model for the post-COVID-19 pandemic.
SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site
Authors: Wang Shiyu , Wang Yanan , Liu Xiangnan , Yang Yongbin , Wu Sufang , Liu Yuan
Published: April 2022

Background: Sulforaphane (SFN) is one kind of phytochemical anticancer drug. It inhibits cancer cell proliferation and promotes cell apoptosis while the mechanism behind is still uncertain. We aimed to explore its downstream target and the radiotherapy sensitization mechanism in cervical cancer. Methods: We treated established cervical cancer cells line (SiHa, HeLa, C33A) with SFN followed by irradiation, and explored its survival, apoptosis, and DNA damage repair in vitro and validated the radiosensitivity of SFN treatment in vivo. We conducted mRNA sequencing to identify differentially expressed mRNAs after SFN treatment. We further investigated SFN downstream target and its involvement in DNA damage repair under irradiation. Results: We found that SFN inhibited the survival of cervical cancer cells under radiotherapy treatment in vitro and prolonged the survival period after radiotherapy in the mouse tumorigenic model. SFN increased the protein expression of LATS2 and promoted apoptosis of cervical cancer cells. Overexpressed LATS2 decreased the cellular survival rate of cervical cancer cells. Additionally, SFN treatment and LATS2 overexpression prevented MDC1 and Rad51 from accumulating in the nucleus in cervical cancer cells after being exposed to ionized radiation. LATS2 loss intervened with SFN-alleviated RAD51 and MDC1 nucleus accumulation and resumed the repairment of DNA damage. Conclusion: We identified SFN as cervical cancer cells radiotherapy sensitizer and LATS2 served as a downstream target of SFN treatment. SFN treatment resulted in the inhibition of the homologous recombination (HR) pathway, and LATS2 has an indispensable contribution to this SFN-facilitated radiotherapy sensitization.
Correction: Takakura et al. In Vitro and In Vivo Cell Uptake of a Cell-Penetrating Peptide Conjugated with Fluorescent Dyes Having Different Chemical Properties. Cancers 2021, 13, 2245
Authors: Takakura Hideo , Sato Honoka , Nakajima Kohei , Suzuki Motofumi , Ogawa Mikako
Published: April 2022

There was an error in the description in the original publication, and it contained information that should not have been disclosed [...]
Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy
Authors: Gwak Eun , Kim Dasol , Hwang Hui-Yun , Kwon Ho
Published: April 2022

Human colon carcinomas, including HCT116 cells, often exhibit high autophagic flux under nutrient deprivation or hypoxic conditions. Mitochondrial ROS (mROS) is known as a ‘molecular switch’ for regulating the autophagic pathway, which is critical for directing cancer cell survival or death. In early tumorigenesis, autophagy plays important roles in maintaining cellular homeostasis and contributes to tumor growth. However, the relationships between mROS and the autophagic capacities of HCT116 cells are poorly understood. Ubiquinol cytochrome c reductase binding protein (UQCRB) has been reported as a biomarker of colorectal cancer, but its role in tumor growth has not been clarified. Here, we showed that UQCRB is overexpressed in HCT116 cells compared to CCD18co cells, a normal colon fibroblast cell line. Pharmacological inhibition of UQCRB reduced mROS levels, autophagic flux, and the growth of HCT116 tumors in a xenograft mouse model. We further investigated mutant UQCRB-overexpressing cell lines to identify functional links in UQCRB-mROS-autophagy. Notably, an increasing level of mROS caused by UQCRB overexpression released Ca2+ by the activation of lysosomal transient receptor potential mucolipin 1 (TRPML1) channels. This activation induced transcription factor EB (TFEB) nuclear translocation and lysosome biogenesis, leading to autophagy flux. Collectively, our study showed that increasing levels of mROS caused by the overexpression of UQCRB in human colon carcinoma HCT116 cells could be linked to autophagy for cell survival.
Telemedicine and Its Past, Present, and Future Roles in Providing Palliative Care to Advanced Cancer Patients
Authors: Tang Michael , Reddy Akhila
Published: April 2022

The landscape of healthcare delivery has considerably changed due to the emergence of coronavirus disease 2019 (COVID-19). This is nowhere more evident than in the care of advanced cancer patients receiving palliative care. This population is susceptible to the severe complications of COVID-19, and immediate measures had to be taken to ensure their safety. Thus, the adoption of telemedicine as a health care delivery model emerged. This model provides many benefits, such as improved access to care while maintaining social distancing; however, there exist challenges to this model, including health care disparities, reimbursement, and monitoring of opioids in high-risk populations. This narrative review provides an overview of the unique benefits and barriers of telemedicine in palliative care patients.
Are Survival Outcomes Different for Young and Old Patients with Oral and Oropharyngeal Squamous Cell Carcinoma? A Systematic Review and Meta-Analysis
Authors: Panda Swagatika , Mohanty Neeta , Panda Saurav , Mishra Lora , Gopinath Divya , Sahoo Alkananda , Nagraj Sumanth Kumbargere , Lapinska Barbara
Published: April 2022

This systematic review and meta-analysis aims to address whether age can be a determinant of overall survival (OS), disease-free survival (DFS), recurrence, distant metastasis (DM) and second primary (SP) in surgically treated oral and oropharyngeal squamous cell carcinoma (OOPSCC). A total of 4981 cases and 44254 controls from 25 comparative observational studies were included in the analysis. A significantly better OS (matched subgroup analysis: OR 1.64; 95% CI 1.31–2.04, overall analysis: OR 1.48; 95% CI 1.09–2.01) was observed in young patients compared to older adults, with heterogeneity ranging from moderate to severe. Worse DFS (unmatched subgroup analysis OR 0.43; 95% CI 0.27–0.68) was observed in young patients compared to older adults with minimal to moderate heterogeneity. The frequency of recurrence (OR 1.49; 95% CI 1.10–2.02) and DM (OR 1.83; 95% CI 1.10–3.03) was significantly higher in the young patients, as found in unmatched and matched subgroup analysis, with the least heterogeneities. Young age can be considered as an independent prognostic factor for recurrence and distant metastases in OOP-SCC. Larger and methodologically robust observational studies with longer follow-up are needed to establish the definitive role of age as an independent prognostic factor on OS and DFS in OOPSCC.
Radiomics Analysis on [68Ga]Ga-PSMA-11 PET and MRI-ADC for the Prediction of Prostate Cancer ISUP Grades: Preliminary Results of the BIOPSTAGE Trial
Authors: Feliciani Giacomo , Celli Monica , Ferroni Fabio , Menghi Enrico , Azzali Irene , Caroli Paola , Matteucci Federica , Barone Domenico , Paganelli Giovanni , Sarnelli Anna
Published: April 2022

Prostate cancer (PCa) risk categorization based on clinical/PSA testing results in a substantial number of men being overdiagnosed with indolent, early-stage PCa. Clinically non-significant PCa is characterized as the presence of ISUP grade one, where PCa is found in no more than two prostate biopsy cores.MRI-ADC and [68Ga]Ga-PSMA-11 PET have been proposed as tools to predict ISUP grade one patients and consequently reduce overdiagnosis. In this study, Radiomics analysis is applied to MRI-ADC and [68Ga]Ga-PSMA-11 PET maps to quantify tumor characteristics and predict histology-proven ISUP grades. ICC was applied with a threshold of 0.6 to assess the features’ stability with variations in contouring. Logistic regression predictive models based on imaging features were trained on 31 lesions to differentiate ISUP grade one patients from ISUP two+ patients. The best model based on [68Ga]Ga-PSMA-11 PET returned a prediction efficiency of 95% in the training phase and 100% in the test phase whereas the best model based on MRI-ADC had an efficiency of 100% in both phases. Employing both imaging modalities, prediction efficiency was 100% in the training phase and 93% in the test phase. Although our patient cohort was small, it was possible to assess that both imaging modalities add information to the prediction models and show promising results for further investigations.
Deciphering HER2-HER3 Dimerization at the Single CTC Level: A Microfluidic Approach
Authors: Tulukcuoglu Guneri Ezgi , Lakis Emile , Hajji Ismail , Martin Elian , Champ Jerome , Rampanou Aurore , Pierga Jean-Yves , Viovy Jean-Louis , Proudhon Charlotte , Bidard François-Clément , Descroix Stéphanie
Published: April 2022

Microfluidics has provided clinicians with new technologies to detect and analyze circulating tumor biomarkers in order to further improve their understanding of disease mechanism, as well as to improve patient management. Among these different biomarkers, circulating tumor cells have proven to be of high interest for different types of cancer and in particular for breast cancer. Here we focus our attention on a breast cancer subtype referred as HER2-positive breast cancer, this cancer being associated with an amplification of HER2 protein at the plasma membrane of cancer cells. Combined with therapies targeting the HER2 protein, HER2-HER3 dimerization blockade further improves a patient’s outcome. In this work, we propose a new approach to CTC characterization by on-chip integrating proximity ligation assay, so that we can quantify the HER2-HER3 dimerization event at the level of single CTC. To achieve this, we developed a microfluidic approach combining both CTC capture, identification and HER2-HER3 status quantification by Proximity Ligation Assay (PLA). We first optimized and demonstrated the potential of the on-chip quantification of HER2-HER3 dimerization using cancer cell lines with various levels of HER2 overexpression and validated its clinical potential with a patient’s sample treated or not with HER2-targeted therapy.
Oestrogen Receptor Isoforms May Represent a Therapeutic Target in Oesophageal Adenocarcinoma
Authors: Due Steven L. , Watson David I. , Bastian Isabell , Eichelmann Ann-Kathrin , Hussey Damian J.
Published: April 2022

Oesophageal adenocarcinoma is a rapidly increasing problem in which treatment options are limited. Previous studies have shown that oesophageal adenocarcinoma cells and tissues express oestrogen receptors (ERs) and show growth suppression and apoptosis in response to ER modulator agents such as tamoxifen. ERs are known to be expressed in a number of isoforms that act together to regulate cell growth and cell death. In this study, we used western blotting to profile the expression of ERα and ERβ isoforms, and expression of the oncologically related molecules p53, HER2, and EGFR, in a panel of oesophageal adenocarcinoma cell lines. The cytotoxicity of tamoxifen in the cell lines was determined with Annexin V-FITC flow cytometry, and correlations between cytotoxicity and receptor expression were assessed using Spearman’s rank-order correlation. Oesophageal adenocarcinoma cell lines showed varying cytotoxicity in response to tamoxifen. The ER species ERα90, ERα50, and ERα46, as well as p53, were positively associated with a cytotoxic response. Conversely, ERα74, ERα70, and ERβ54 were associated with a lack of cytotoxic response. The ER species detected in oesophageal adenocarcinoma cells may work together to confer sensitivity to ER modulators in this disease, which could open up a new avenue for therapy in selected patients.
Reliability of International Fitness Scale (IFIS) in Chinese Children and Adolescents
Authors: Bao Ran , Chen Sitong , Kastelic Kaja , Drenowatz Clemens , Li Minghui , Zhang Jialin , Wang Lei
Published: April 2022

Background and Objectives: It has previously been shown that the International Fitness Scale (IFIS) is a reliable and valid instrument when used in numerous regions and subgroups, but it remains to be determined whether the IFIS is a reliable instrument for use with Chinese children and adolescents. If the reliability of the IFIS can be verified, populational surveillance and monitoring of physical fitness (PF) can easily be conducted. This study aimed to test the reliability of the IFIS when used with Chinese children and adolescents. Methods: The convenience sampling method was used to recruit study participants. In total, 974 school-aged children and adolescents between 11 and 17 years of age were recruited from three cities in Southeast China: Shanghai, Nanjing and Wuxi. The study participants self-reported demographic data, including age (in years) and sex (boy or girl). The participants completed the questionnaire twice within a two-week interval. Results: A response rate of 95.9% resulted in a sample of 934 participants (13.7 ± 1.5 years, 47.4% girls) with valid data. On average, the participants were 13.7 ± 1.5 years of age. The test–retest weighted kappa coefficients for overall fitness, cardiorespiratory fitness, muscle fitness, speed and agility and flexibility were 0.52 (Std. errs. = 0.02), 0.51 (Std. errs. = 0.02), 0.60 (Std. errs. = 0.02), 0.55 (Std. errs. = 0.02) and 0.55 (Std. errs. = 0.02), respectively. Conclusions: The International Fitness Scale was found to have moderate reliability in the assessment of (self-reported) physical fitness in Chinese children and adolescents. In the future, the validity of the IFIS should be urgently tested in Chinese subgroup populations.
Numerical and Experimental Investigation of Anchor Channels Subjected to Tension Load in Composite Slabs with Profiled Steel Decking
Authors: Bogdanić Anton , Casucci Daniele , Ožbolt Joško
Published: April 2022

In curtain wall applications, anchor channels are frequently installed near the edge of composite slabs with profiled steel decking. The complex concrete geometry of these floor slabs affects the capacity of all concrete failure modes, but there are currently no guidelines or investigations available on this topic. The main objective of the present research is to investigate how the position of anchor channels and the complex slab geometry influence the tensile capacity of anchor channels. For this purpose, an extensive numerical parametric study was performed using the 3D nonlinear FE code MASA, which is based on the microplane constitutive model. In order to validate the numerical results, an experimental program was carried out for some of the configurations possible in practice. Based on the results, recommendations are given for the reduction in the tensile capacity of anchor channels in composite slabs with profiled steel decking.
Exploring AMOC Regime Change over the Past Four Decades through Ocean Reanalyses
Authors: de Toma Vincenzo , Artale Vincenzo , Yang Chunxue
Published: April 2022

We examine North Atlantic climate variability using an ensemble of ocean reanalysis datasets to study the Atlantic Meridional Overturning Circulation (AMOC) from 1979 to 2018. The dataset intercomparison shows good agreement for the latest period (1995–2018) for AMOC dynamics, characterized by a weaker overturning circulation after 1995 and a more intense one during 1979–1995, with varying intensity across the various datasets. The correlation between leading empirical orthogonal functions suggests that the AMOC weakening has connections with cooler (warmer) sea surface temperature (SST) and lower (higher) ocean heat content in the subpolar (subtropical) gyre in the North Atlantic. Barotropic stream function and Gulf Stream index reveal a shrinking subpolar gyre and an expanding subtropical gyre during the strong-AMOC period and vice versa, consistently with Labrador Sea deep convection reduction. We also observed an east–west salt redistribution between the two periods. Additional analyses show that the AMOC variability is related to the North Atlantic Oscillation phase change around 1995. One of the datasets included in the comparison shows an overestimation of AMOC variability, notwithstanding the model SST bias reduction via ERA-Interim flux adjustments: further studies with a set of numerical experiments will help explain this behavior.
A Novel SERS Substrate Based on Discarded Oyster Shells for Rapid Detection of Organophosphorus Pesticide
Authors: Chu Chi-Yu , Lin Pei-Ying , Li Jun-Sian , Kirankumar Rajendranath , Tsai Chen-Yu , Chen Nan-Fu , Wen Zhi-Hong , Hsieh Shuchen
Published: April 2022

Over the past few years, the concern for green chemistry and sustainable development has risen dramatically. Researchers make an effort to find solutions to difficult challenges using green chemical processes. In this study, we use oyster shells as a green chemical source to prepare calcium oxide nanoparticles (CaO-NPs). Transmission electron microscopy (TEM) results showed the CaO-NPs morphology, which was spherical in shape, 40 ± 5 nm in diameter, with uniform dispersion. We further prepared silver/polydopamine/calcium-oxide (Ag/PDA/CaO) nanocomposites as the surface-enhanced Raman scattering (SERS) substrates and evaluated their enhancement effect using the methyl parathion pesticide. The effective SERS detection limit of this method is 0.9 nM methyl parathion, which is much lower than the safety limits set by the Collaborative International Pesticides Analytical Council for insecticide in fruits. This novel green material is an excellent SERS substrate for future applications and meets the goal of green chemistry and sustainable development.
Corrosion Resistance of Li-Al LDHs Film Modified by Methionine for 6063 Al Alloy in 3.5 wt.% NaCl Solution
Authors: Ma Ji , Chang Menglei , He Haiying , Wei Hongyang , Huang Yinchun , Du Xiaoqing , Chen Dongchu
Published: April 2022

Methionine (Met) was introduced to modify the Li-Al layered double hydroxides (LDHs) film prepared on 6063 aluminum alloy by in situ method for the first time. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, Scanning electron microscopy, and X-ray diffraction confirmed the successful insertion of Met into LDHs film and revealed that the introduction of Met could make the LDHs film much denser. Electrochemical tests illustrated that the corrosion rate of the Met modified LDHs film was reduced by more than an order of magnitude compared with the bare Al alloy. Moreover, the corrosion rate of the modified LDHs film after immersion in 3.5 wt.% NaCl solution for 21 days was almost the same as that without immersion, which indicates that the modified film has good corrosion durability. The corrosion resistance of the scratched modified film could recover to the level without a scratch on the 14th day based on the scratch test results, meaning the modified film has a good self-healing property. Finally, the anti-corrosion mechanism of the Met was proved by molecular dynamic simulations and found that the enhanced corrosion resistance may be attributed to the addition of Met that slowed the diffusion of the corrosive medium Cl− and water molecules.
Isolation Forests and Deep Autoencoders for Industrial Screw Tightening Anomaly Detection
Authors: Ribeiro Diogo , Matos Luís Miguel , Moreira Guilherme , Pilastri André , Cortez Paulo
Published: April 2022

Within the context of Industry 4.0, quality assessment procedures using data-driven techniques are becoming more critical due to the generation of massive amounts of production data. In this paper, we address the detection of abnormal screw tightening processes, which is a key industrial task. Since labeling is costly, requiring a manual effort, we focus on unsupervised detection approaches. In particular, we assume a computationally light low-dimensional problem formulation based on angle–torque pairs. Our work is focused on two unsupervised machine learning (ML) algorithms: isolation forest (IForest) and a deep learning autoencoder (AE). Several computational experiments were held by assuming distinct datasets and a realistic rolling window evaluation procedure. First, we compared the two ML algorithms with two other methods, a local outlier factor method and a supervised Random Forest, on older data related with two production days collected in November 2020. Since competitive results were obtained, during a second stage, we further compared the AE and IForest methods by adopting a more recent and larger dataset (from February to March 2021, totaling 26.9 million observations and related to three distinct assembled products). Both anomaly detection methods obtained an excellent quality class discrimination (higher than 90%) under a realistic rolling window with several training and testing updates. Turning to the computational effort, the AE is much lighter than the IForest for training (around 2.7 times faster) and inference (requiring 3.0 times less computation). This AE property is valuable within this industrial domain since it tends to generate big data. Finally, using the anomaly detection estimates, we developed an interactive visualization tool that provides explainable artificial intelligence (XAI) knowledge for the human operators, helping them to better identify the angle–torque regions associated with screw tightening failures.
Review of Electrochemical Testing to Assess Corrosion of Post-Tensioned Tendons with Segregated Grout
Authors: Permeh Samanbar , Lau Kingsley
Published: April 2022

Post-tensioned (PT) construction incorporating bonded tendons with cementitious grouts has been used for highway bridges. The tendon duct and the encapsulating grout materials provide barrier corrosion protection for the embedded high-strength steel strand. Although generally used in good engineering practice, cases of PT tendon corrosion have been documented relating to inadequate detailing for joints and development of grout bleed water. Recently, in the past several years, unexpected severe localized strand corrosion has related to the segregation of thixotropic grouts. In the latter case, thixotropic grouts (that have been developed to mitigate grout bleeding) formed physical and chemical deficiencies that have been characterized to have high moisture content and elevated sulfate ion concentrations. The early presence of elevated sulfate ion concentrations in the deficient grout hinders stable steel passivation. The corrosion mechanism can be complicated due to the compounding effects of physical grout deficiency, moisture content, pore water pH, and the presence of sulfate ions. There remains interest to reliably assess corrosion of PT tendons with deficient grout. A review of electrochemical techniques and test methods used in earlier research by the authors to identify the role of sulfates on localized steel corrosion in alkaline solutions is presented. It was evident that different testing methods can reveal various aspects of the corrosion of strands in the deficient PT grout. The open-circuit potential and linear polarization method could differentiate corrosion activity between hardened and deficient grout environments but did not reveal the development of localized corrosion. Electrochemical impedance spectroscopy was useful to identify grout deficiencies by the differentiation of its bulk electrical properties. Potentiodynamic polarization and electrochemical noise technique were used to identify metastable and pitting in alkaline sulfate solutions representative of the deficient grout pore water.