Disrupted topological properties of structural brain networks present a glutamatergic neuropathophysiology in people with narcolepsy
Authors: Chen Guoyan , Wang Wen , Wu Haoyang , Zhao Xiangchao , Kang Xiaopeng , Ren Jiafeng , Zhang Jun , He Jiaxiu , Sun Shihui , Zhong Zhao , Shang Danqing , Fan Mengmeng , Cheng Jinxiang , Zhang Dan , Su Changjun , Lin Jiaji 
Published: July 2023

Study objectives

Growing evidences have documented various abnormalities of the white matter bundles in people with narcolepsy. We sought to evaluate topological properties of brain structural networks, and their association with symptoms and neuropathophysiological features in people with narcolepsy.

Methods

Diffusion tensor imaging (DTI) was conducted for people with narcolepsy (n = 30) and matched healthy controls as well as symptoms assessment. Structural connectivity for each participant was generated to analyze global and regional topological properties and their correlations with narcoleptic features. Further human brain transcriptome was extracted and spatially registered for connectivity vulnerability. Genetic functional enrichment analysis was performed and further clarified usingin vivoemission computed tomography data.

Results

A wide and dramatic decrease in structural connectivities was observed in people with narcolepsy, with descending network degree and global efficiency. These metrics were not only correlated with sleep latency and awakening features, but also reflected alterations of sleep macrostructure in people with narcolepsy. Network-based statistics identified a small hyperenhanced subnetwork of cingulate gyrus that was closely related to rapid eye movement sleep behavior disorder (RBD) in narcolepsy. Further imaging genetics analysis suggested glutamatergic signatures were responsible for the preferential vulnerability of connectivity alterations in people with narcolepsy, while additional PET/SPECT data verified that structural alteration was significantly correlated with metabotropic glutamate receptor 5 (mGlutR5) and N-methyl-D-aspartate receptor (NMDA).

Conclusions

People with narcolepsy endured a remarkable decrease in the structural architecture, which was not only be closely related to narcolepsy symptoms but also glutamatergic signatures.

Statement of Significance

Growing evidences have identified a widespread disrupted white matter integrity of people with narcolepsy, so that connectome properties and neuropathophysiological features underlying these abnormalities have become a topic of increasing interest. This report extends on findings regarding the structural wirings and architectural topology of people with narcolepsy and inferring their clinical correlation with sleepiness assessment, polysomnography features and sleep macrostructure. Further imaging genetics analysis suggests glutamatergic signatures are responsible for the preferential vulnerability of connectivity alterations, while additional PET/SPECT data verifies that structural alteration is significantly correlated with metabotropic glutamate receptor 5 (mGlutR5) and N-methyl-D-aspartate receptor (NMDA). Our findings, therefore, converge structural network and genetic signatures for in people with narcolepsy.