bluuebunny/crossref_metadata_2025 huggingface dataset:
Applying an Evolutionary Approach of Risk-Taking Behaviors in Adolescents
Authors: Salas-Rodríguez Javier , Gómez-Jacinto Luis , Hombrados-Mendieta Isabel , del Pino-Brunet Natalia
Published: January 2022

Risk-taking behaviors in adolescents have traditionally been analyzed from a psychopathological approach, with an excessive emphasis on their potential costs. From evolutionary theory we propose that risk-taking behaviors can be means through which adolescents obtain potential benefits for survival and reproduction. The present study analyses sex differences in three contexts of risk (i.e., risk propensity, expected benefits and risk perception) in the evolutionary specific domains and the predictive value of these domains over risk-taking behaviors, separately in female and male adolescents. 749 adolescents (females = 370) valued their risk perception, expected benefits and risk propensity through the Evolutionary Domain-Specific Risk Scale, as well as their engagement in risk-taking behaviors through the Risky Behavior Questionnaire. Male adolescents showed lower risk perception in two evolutionary domains, expected higher benefits in two other domains and showed higher risk propensity in six domains. Female adolescents showed lower risk perception in two domains. Additionally, risk perception, expected benefits and risk propensity in the evolutionary domains predicted the engagement in risk-taking behaviors in male adolescents, whereas in female adolescents only expected benefits and risk propensity showed a predictive effect over risk-taking behaviors. These results suggest the potential role of evolutionary mechanisms on risk-taking behaviors in adolescents. Results have practical implications for interventions programs aimed at reducing risk-taking behaviors. In addition to considering sex differences, intervention programs should consider alternative behaviors through which adolescents can reach their evolutionary goals, and handle the risks related to those behaviors that cannot be replaced but have potential benefits for adolescents.
The Six Components of Social Interactions: Actor, Partner, Relation, Activities, Context, and Evaluation
Authors: Hoppler Sarah Susanna , Segerer Robin , Nikitin Jana
Published: January 2022

Social interactions are essential aspects of social relationships. Despite their centrality, there is a lack of a standardized approach to systematize social interactions. The present research developed (Study 1) and tested (Study 2) a taxonomy of social interactions. In Study 1 (5,676 descriptions of social interactions from N = 708 participants, age range 18–83 years), we combined a bottom-up approach based on the grounded theory with a top-down approach integrating existing empirical and theoretical literature to develop the taxonomy. The resulting taxonomy (APRACE) comprises the components Actor, Partner, Relation, Activities, Context, and Evaluation, each specified by features on three levels of abstraction. A social situation can be described by a combination of the components and their features on the respective abstraction level. Study 2 tested the APRACE using another dataset (N = 303, age range 18–88 years) with 1,899 descriptions of social interactions. The index scores of the six components, the frequencies of the features on the most abstract level, and their correlations were largely consistent across both studies, which supports the generalizability of the APRACE. The APRACE offers a generalizable tool for the comprehensive, parsimonious, and systematic description of social interactions and, thus, enables networked research on social interactions and application in a number of practical fields.
Webcams and Social Interaction During Online Classes: Identity Work, Presentation of Self, and Well-Being
Authors: Hosszu Alexandra , Rughiniş Cosima , Rughiniş Răzvan , Rosner Daniel
Published: January 2022

The well-being of children and young people has been affected by the COVID-19 pandemic. The shift to online education disrupted daily rhythms, transformed learning opportunities, and redefined social connections with peers and teachers. We here present a qualitative content analysis of responses to open-ended questions in a large-scale survey of teachers and students in Romania. We explore how their well-being has been impacted by online education through (1) overflow effects of the sudden move to online classes; (2) identity work at the individual and group levels; and (3) Students’ and teachers’ presentations of self in the online environment, with a focus on problematic aspects of webcam use. The results indicate that both students and teachers experienced ambivalence and diverse changes in well-being, generated by the flexibility, burdens, and disruptions of school-from-home. The identities associated with the roles of teacher and student have been challenged and opened for re-negotiation. Novel patterns have emerged in teachers’ and Students’ identity work. Failure or success at the presentation of self in online situations is relevant for the emotional valence of learning encounters, impacting well-being. Online classes have brought about new ways to control one’s presentation of self while also eliminating previous tactics and resources. The controversy regarding webcams has captured this duality: for some, the home remained a backstage that could not be safely exposed; for others, the home became a convenient front stage for school. Well-being was affected by the success of individual and collective performances, and by student-teacher asymmetries. Overall, our study of online learning indicates powerful yet variable influences on subjective well-being, which are related to overflow effects, identity work, and presentation of self.
The Mediating Effect of Creativity on the Relationship Between Mathematic Achievement and Programming Self-Efficacy
Authors: Liu Jun , Sun Meng , Dong Yue , Xu Fei , Sun Xue , Zhou Yan
Published: January 2022

Purpose: This study aimed to explore the relationship between mathematic achievement and programming self-efficacy, and adopt a mediation model to verify the mediating role of creativity on the relationship between mathematic achievement and programming self-efficacy.

Methods: A total of 950 upper-secondary school students were surveyed using their math test scores, the Kirton Adaption-Innovation and the Programmed Self-Efficacy Scale. SPSS-26 was used for descriptive statistical analysis and correlation analysis of related variables. The PROCESS plugin was used to test the mediating effect of creativity.

Results: (1) Mathematic achievement has a positive effect on programming self-efficacy, mathematic achievement is positively related to creativity, and creativity also has a positive influence on programming self-efficacy. (2) Creativity has a mediating effect on the relationship between mathematic achievement and programming self-efficacy.

Conclusion: The results revealed that mathematic achievement affected programming self-efficacy directly and also indirectly through creativity. This provided certain ideas for the development of programming education for teenagers. Since students’ mathematics learning and creativity are related to programming learning, it is necessary to pay attention to the integration of the disciplines of programming education and mathematics. Further, the cultivation of innovative thinking is also critical to facilitate programming learning.
Identity Work as Ethical Self-Formation: The Case of Two Chinese English-as-Foreign-Language Teachers in the Context of Curriculum Reform
Authors: Jiang Anne Li
Published: January 2022

Curriculum reform urges teachers to constantly reflect on existing identities and develop probably whole new identities. Yet, in the wake of the poststructuralist view of identity as a complex matter of the social and the individual, of discourse and practice, and of agency and structure, teacher identity is a process of arguing for themselves and hence ethical and political in nature. Drawing on Foucault’s notion of ethical self-formation and its adoption by Clarke (2009a) “Diagram for Doing Identity Work” in teacher education research, this 2-year-long case study explores how two Chinese English-as-foreign-language (EFL) teachers engaged in identity work in a changing curricular landscape. The analysis of narrative frames and semistructured interviews reveals the relations between the relative stable and the evolving elements of teachers’ identity work, and the essential role of teachers’ ethical agency based on reflective and critical responsiveness to the contextual reality and the dynamic power relations during the reform. The findings argue for the importance of nourishing teachers’ reflective identity work and ethical agency during the turbulence of educational change.
Word- and Text-Level Processes Contributing to Fluent Reading of Word Lists and Sentences
Authors: van Viersen Sietske , Protopapas Athanassios , de Jong Peter F.
Published: January 2022

In this study, we investigated how word- and text-level processes contribute to different types of reading fluency measures. We aimed to increase our understanding of the underlying processes necessary for fluent reading. The sample included 73 Dutch Grade 3 children, who were assessed on serial word reading rate (familiar words), word-list reading fluency (increasingly difficult words), and sentence reading fluency. Word-level processes were individual word recognition speed (discrete word reading) and sequential processing efficiency (serial digit naming). Text-level processes were receptive vocabulary and syntactic skills. The results showed that word- and text-level processes combined accounted for a comparable amount of variance in all fluency outcomes. Both word-level processes were moderate predictors of all fluency outcomes. However, vocabulary only moderately predicted sentence reading fluency, and syntactic skills merely contributed to sentence reading fluency indirectly through vocabulary. The findings indicate that sequential processing efficiency has a crucial role in reading fluency across various measures besides individual word recognition speed. Additionally, text-level processes come into play when complexity and context availability of fluency measures increases, but the exact timing requires further study. Findings are discussed in terms of future directions and their possible value for diagnostic assessment and intervention of reading difficulties.
Preliminary Study on a Novel Protocol for Improving Familiarity with a Lower-Limb Robotic Exoskeleton in Able-Bodied, First-Time Users
Authors: Lau Jan C. L. , Mombaur Katja
Published: January 2022

Lower-limb exoskeletons have been created for different healthcare needs, but no research has been done on developing a proper protocol for users to get accustomed to moving with one. The user manuals provided also do not include such instructions. A pre-test was conducted with the TWIN (IIT), which is a lower-limb exoskeleton made for persons with spinal cord injury. In the pre-test, two healthy, able-bodied graduate students indicated a need for a protocol that can better prepare able-bodied, first-time users to move with an exoskeleton. TWIN was used in this preliminary study and nine users were divided to receive a tutorial or no tutorial before walking with the exoskeleton. Due to COVID-19 regulations, the study could only be performed with healthy, young-to-middle-aged lab members that do not require walking support. The proposed protocol was evaluated with the System Usability Scale, NASA Raw Task Load Index, and two custom surveys. The members who received the tutorial found it easy to follow and helpful, but the tutorial seemed to come at a price of higher perceived mental and physical demands, which could stem from the longer testing duration and the need to constantly recall and apply the things learned from the tutorial. All results presented are preliminary, and it is recommended to include biomechanical analysis and conduct the experiment with more participants in the future. Nonetheless, this proof-of-concept study lays groundwork for future related studies and the protocol will be adjusted, applied, and validated to patients and geriatric users.
Functional Characterization of an Amaranth Natterin-4-Like-1 Gene in Arabidopsis thaliana
Authors: Cabrales-Orona Gabriela , Martínez-Gallardo Norma , Délano-Frier John Paul
Published: January 2022

The functional characterization of anAmaranthus hypochondriacus Natterin-4-Like-1gene (AhN4L-1) coding for an unknown function protein characterized by the presence of an aerolysin-like pore-forming domain in addition to two amaranthin-like agglutinin domains is herewith described. Natterin and nattering-like proteins have been amply described in the animal kingdom. However, the role of nattering-like proteins in plants is practically unknown. The results described in this study, obtained from gene expression data in grain amaranth and fromAhN4L-1-overexpressingArabidopsis thalianaplants indicated that this gene was strongly induced by several biotic and abiotic conditions in grain amaranth, whereas data obtained from the overexpressing Arabidopsis plants further supported the defensive function of this gene, mostly against bacterial and fungal plant pathogens. GUS and GFPAhN4L-1localization in roots tips, leaf stomata, stamens and pistils also suggested a defensive function in these organs, although its participation in flowering processes, such as self-incompatibility and abscission, is also possible. However, contrary to expectations, the overexpression of this gene negatively affected the vegetative and reproductive growth of the transgenic plants, which also showed no increased tolerance to salinity and water-deficit stress. The latter despite the maintenance of significantly higher chlorophyll levels and photosynthetic parameters under intense salinity stress. These results are discussed in the context of the physiological roles known to be played by related lectins and AB proteins in plants.
Coupling Effect of Nonlinear Stiffness of Tape Spring Hinges and Flexible Deformation of Panels during Orbit Maneuvers
Authors: Gu Wenyan , Zhang Jinsheng , Pan Longye , Qu Yegao , Choi Jin-Hwan , Zhu Xiangqian
Published: January 2022

Many solar panels for spacecrafts are deployed by Tape Spring Hinges (TSHs) which have changeable stiffness. The stiffness of TSH is small when panels are folded, and it becomes large quickly in its deployed status. Since the solar panel is a thin sheet, flexible deformation is easily generated by orbit maneuvers. The coupling effect between the nonlinear TSHs and the flexible panels generates obvious vibration which affects the operational stability of the satellite. To investigate this coupling effect, non-deformable, linear deformable and nonlinear deformable panels were modelled by rigid body, modal order reduction method (MORM) and finite element method (FEM), respectively. The driving torque of TSH was described as a function of the rotation angle and angular velocity. The nonlinear properties of the TSH were reflected by one angle-stiffness spline multiplied by one stiffness coefficient. Dynamic responses of a satellite in deployment and orbit steering were analyzed by numerical simulations. Analysis results indicate the local deformation of panels keeps the stiffness of the TSH within a large range which accelerates the orbit maneuvers. However, much vibration is generated by the coupling effect if the luck-up status is broken up. The coupling effect affects the sequence of deployment, overshoot phenomenon and acceleration magnitude of the panels. Although the MORM is more efficient than FEM in computation, we propose FEM is better suited in the design of TSH and in studying the precise control of spacecraft with flexible solar panels and TSHs.
Short-Term Abandonment versus Mowing in a Mediterranean-Temperate Meadow: Effects on Floristic Composition, Plant Functionality, and Soil Properties—A Case Study
Authors: Vannucchi Francesca , Lazzeri Valerio , Rosellini Irene , Scatena Manuele , Caudai Claudia , Bretzel Francesca
Published: January 2022

Hay meadows are secondary grasslands maintained by mowing, and their ecological importance resides in the inherent biodiversity and carbon stocking. We investigated the plant community and soil properties of a sub humid acid grassland near the Fucecchio marshes (Italy), managed as a hay meadow, mowed once a year, and not fertilized. Part of the meadow had been abandoned for three years. We analysed the soil properties (i.e., organic carbon and total nitrogen content, available phosphorus, pH, cation-exchange capacity, texture, and conductibility) and the plant community structure (composition, functionality, and species richness) of the two sides of the meadow (mowed and abandoned). Our aim was to highlight the changes in soil properties and vegetation community, and to find out to what extent abandonment can affect those dynamics. Our results showed that after short-term abandonment, soil pH, C and N increased; litter biomass and perennial forbs increased; and annual forbs decreased. New species colonising after abandonment, thus enriching the flora, may keep spreading and eventually hinder the growth of the specialists if mowing is not resumed. Certain valuable meadow habitats need constant human intervention to maintain their peculiar vegetation, most especially if they are a buffer zone in the proximity of natural protected areas.
Effectiveness of Common Preprocessing Methods of Time Series for Monitoring Crop Distribution in Kenya
Authors: Ni Rui , Zhu Xiaohui , Lei Yuping , Li Xiaoxin , Dong Wenxu , Zhang Chuang , Chen Tuo , Mburu David M. , Hu Chunsheng
Published: January 2022

Accurate crop identification and spatial distribution mapping are important for crop production estimation and famine early warning, especially for food-deficit African agricultural countries. By evaluating existing preprocessing methods for classification using satellite image time series (SITS) in Kenya, this study aimed to provide a low-cost method for cultivated land monitoring in sub-Saharan Africa that lacks financial support. SITS were composed of a set of MODIS Vegetation Indices (MOD13Q1) in 2018, and the classification method included the Support Vector Machine (SVM) and Random Forest (RF) classifier. Eight datasets obtained at three levels of preprocessing from MOD13Q1 were used in the classification: (1) raw SITS of vegetation indices (R-NDVI, R-EVI, and R-NDVI + R-EVI); (2) smoothed SITS of vegetation indices (S-NDVI); and (3) vegetation phenological data (P-NDVI, P-EVI, R-NDVI + P-NDVI, and P-NDVI-1). Both SVM and RF classification results showed that the “R-NDVI + R-EVI” dataset achieved the highest performance, while the three pure phenological datasets produced the lowest accuracy. Correlation analysis between variable importance and rainfall time series demonstrated that the vegetation index SITS during rainfall periods showed higher importance in RF classifiers, thus revealing the potential of saving computational costs. Considering the preprocessing cost of SITS and its negative impact on the classification accuracy, we recommend overlaying the original NDVI with the original EVI time series to map the crop distribution in Kenya.
Evaluating the Effect of Cover Materials on Greenhouse Microclimates and Thermal Performance
Authors: Kim Hyung-Kweon , Lee Si-Young , Kwon Jin-Kyung , Kim Yong-Hyeon
Published: January 2022

This study compared and analyzed changes in the microclimate and thermal environment inside single-span greenhouses covered with a single layer of plastic film, polycarbonate (PC), and glass. The results of the experiment show that the PC-covered greenhouse was the most favorable for managing the nighttime heating effect during the cold season. However, the glass-covered greenhouse was found to be the most favorable for managing the cooling effect during the hot season. Although the plastic-covered greenhouse was inexpensive and easy to install, the air temperature inside varied significantly, and it was difficult to control its indoor environment. The thermal load leveling values showed that the PC-covered greenhouse had the lowest variation, confirming its superiority in terms of environmental control and energy savings. In terms of the overall heat transfer, heat was generally transferred from the interior to the exterior of the greenhouses. In the plastic-covered greenhouse, however, heat was transferred in the opposite direction at night due to the influence of radiant cooling. The occurrence of the minimum and maximum heat transfer values had a tendency similar to that of the occurrence of the minimum and maximum air temperatures inside the greenhouses.
Next-Generation Proteomics Reveals a Greater Antioxidative Response to Drought in Coffea arabica Than in Coffea canephora
Authors: Marques Isabel , Gouveia Duarte , Gaillard Jean-Charles , Martins Sónia , Semedo Magda C. , Lidon Fernando C. , DaMatta Fábio M. , Ribeiro-Barros Ana I. , Armengaud Jean , Ramalho José C.
Published: January 2022

Drought is a major threat to coffee, compromising the quality and quantity of its production. We have analyzed the core proteome of 18 Coffea canephora cv. Conilon Clone 153 and C. arabica cv. Icatu plants and assessed their responses to moderate (MWD) and severe (SWD) water deficits. Label-free quantitative shotgun proteomics identified 3000 proteins in both genotypes, but less than 0.8% contributed to ca. 20% of proteome biomass. Proteomic changes were dependent on the severity of drought, being stronger under SWD and with an enrolment of different proteins, functions, and pathways than under MWD. The two genotypes displayed stress-responsive proteins under SWD, but only C. arabica showed a higher abundance of proteins involved in antioxidant detoxification activities. Overall, the impact of MWD was minor in the two genotypes, contrary to previous studies. In contrast, an extensive proteomic response was found under SWD, with C. arabica having a greater potential for acclimation/resilience than C. canephora. This is likely supported by a wider antioxidative response and an ability to repair photosynthetic structures, being crucial to develop new elite genotypes that assure coffee supply under water scarcity levels.
Spring Precipitation Deficiency in Poland and Its Temporal and Spatial Variability in the Context of Agricultural Needs
Authors: Kalbarczyk Robert , Kalbarczyk Eliza
Published: January 2022

Deficient precipitation (dPr) in the growing season, especially in critical periods, affects plant condition and determines the quality and quantity of obtained yields. Knowledge about the variability and distribution of dPr is essential to mitigate its effect on agricultural soils and on crop and livestock production. The goal of the work is to determine the spatial and temporal distribution of spring precipitation deficiency and also to indicate the zones of risk and variability of its occurrence in Poland. It was assumed that dPr occurred when total monthly precipitation in a given year accounted for ≤75% of the total multi-year mean (1951–2018). In the spring season, the multi-year mean of the area covered by deficient precipitation (ACDP) amounted to 33% and fluctuated between approximately 31% in May and approximately 35% in March. The study distinguished four zones in Poland that vary in terms of the risk and variability of spring precipitation deficiency. The obtained results may be used, for example, to assess the needs for irrigation in the changing climate conditions, to model the growing season and yields of cultivated plants, and to select adaptation measures for agriculture in response to climate change.
Expression of Genes Related to Plant Hormone Signal Transduction in Jerusalem Artichoke (Helianthus tuberosus L.) Seedlings under Salt Stress
Authors: Yue Yang , Wang Jueyun , Ren Wencai , Zhou Zhaosheng , Long Xiaohua , Gao Xiumei , Rengel Zed
Published: January 2022

Background: Jerusalem artichoke (Helianthus tuberosus L.) is moderately tolerant to salinity stress and has high economic value. The salt tolerance mechanisms of Jerusalem artichoke are still unclear. Especially in the early stage of Jerusalem artichoke exposure to salt stress, gene transcription is likely to undergo large changes. Previous studies have hinted at the importance of temporal expression analysis in plant transcriptome research. Elucidating these changes may be of great significance to understanding the salt tolerance mechanisms of it. Results: We obtained high-quality transcriptome from leaves and roots of Jerusalem artichoke exposed to salinity (300 mM NaCl) for 0 h (hour), 6 h, 12 h, 24 h, and 48 h, with 150 and 129 unigenes and 9023 DEGs (differentially expressed genes). The RNA-seq data were clustered into time-dependent groups (nine clusters each in leaves and roots); gene functions were distributed evenly among them. KEGG enrichment analysis showed the genes related to plant hormone signal transduction were enriched in almost all treatment comparisons. Under salt stress, genes belonging to PYL (abscisic acid receptor PYR/PYL family), PP2C (Type 2C protein phosphatases), GH3 (Gretchen Hagen3), ETR (ethylene receptor), EIN2/3 (ethylene-insensitive protein 2/3), JAZ (genes such as jasmonate ZIM-domain gene), and MYC2 (Transcription factor MYC2) had extremely similar expression patterns. The results of qRT-PCR of 12 randomly selected and function known genes confirmed the accuracy of RNA-seq. Conclusions: Under the influence of high salinity (300 mM) environment, Jerusalem artichoke suffer serious damage in a short period of time. Based on the expression of genes on the time scale, we found that the distribution of gene functions in time is relatively even. Upregulation of the phytohormone signal transduction had a crucial role in the response of Jerusalem artichoke seedlings to salt stress, and the genes of abscisic acid, auxin, ethylene, and jasmonic acid had the most obvious change pattern. Research emphasized the regulatory role of hormones under high salt shocks and provided an explorable direction for the study of plant salt tolerance mechanisms.
Does Nursing Behaviour of Sows in Loose-Housing Pens Differ from That of Sows in Farrowing Pens with Crates?
Authors: Wiechers Dierck-Hinrich , Herbrandt Swetlana , Kemper Nicole , Fels Michaela
Published: January 2022

Sows confined to farrowing crates are restricted in performing natural behaviour such as maternal behaviour. Loose-housing farrowing pens (LH) and farrowing pens with crates (FC) were compared regarding sows’ nursing behaviour via video analyses over four weeks per batch (one day per week). Nursing frequency was similar in LH and FC pens (1.25 ± 0.82 vs. 1.19 ± 0.75 nursings/sow/hour; p > 0.05). However, nursing duration differed between the two systems (LH: 5.7 ± 4.6 min vs. FC: 7.0 ± 5.0 min; odds ratio (OR) 1.168, p = 0.011). In LH pens, more nursing bouts were sow-terminated than in FC pens (OR 0.427, p = 0.001). The probability of sow-terminated nursing occurring increased from week 1 to week 4 (OR 3.479, adjusted p (padj) < 0.001), while that of observing unnursed piglets decreased from week 1 to week 4 (OR 0.301, padj < 0.001) and rose with increasing litter size (OR 1.174, p = 0.010). We conclude that nursing behaviour was affected by the farrowing system, with shorter nursing duration and more nursing terminations by the sow in LH than in FC pens. Since this corresponds to the nursing behaviour of sows in semi-natural conditions, it can be assumed that sows in LH pens are more likely to exhibit natural nursing behaviour.
Effects of Anthocyanin Supplementation and Ageing Time on the Volatile Organic Compounds and Sensory Attributes of Meat from Goat Kids
Authors: Sgarro Maria Federica , Maggiolino Aristide , Pateiro Mirian , Domínguez Rubén , Iannaccone Francesco , De Palo Pasquale , Lorenzo José M.
Published: January 2022

The aim of this study was to assess the effects of dietary anthocyanin addition on volatile compounds of meat from goat kids during ageing. For this work, 60 male and female kids were divided into two groups: red orange and lemon extract (RLE group; n = 30), which received an RLE extract (90 mg/kg of live weight); and control (CON group; n = 30). The phytoextract in dry powder form was rich in bioflavonoids such as flavanones (about 16%) and anthocyanins (about 3%). After slaughtering, the longissimus thoracis et lumborum muscle was aged at 4 °C. The volatile organic compound (VOC) and sensorial analyses were carried out at 1, 3 and 7 days. A total of 10 chemical families were identified during the ageing process. Aldehydes were the most abundant VOC, followed by ketones and alcohols. Their contents increased during the process, showing after 7 days of ageing mean values of 20,498, 2193 and 1879 ng/g of meat, respectively. Regarding dietary effects, carboxylic acids, hydrocarbons and thiols presented significant differences between treatments, with higher carboxylic acid contents observed in RLE samples (437 vs. 467 ng/g of meat for CON and RLE batches, respectively; p < 0.05). On the contrary, hydrocarbons (436 vs. 254 ng/g of meat for CON and RLE batches, respectively) and thiols (160 vs. 103 ng/g of meat for CON and RLE batches, respectively) displayed significantly (p < 0.01) higher amounts in CON compared to the RLE group. Regarding ageing time, the tenderness, juiciness, odour and overall assessment parameters showed significantly higher scores at the end of the whole process (p < 0.05). On the other hand, only odour displayed significant differences between treatments, reaching higher scores in CON samples (p < 0.05). Therefore, ageing time improved the sensorial properties (tenderness, juiciness, odour and overall assessment) and the VOC content, whereas the inclusion of anthocyanins in the kids’ diet did not have a great impact on the properties of aged meat.
Hormonal Management for the Induction of Luteolysis and Ovulation in Andalusian Jennies: Effect on Reproductive Performance, Embryo Quality and Recovery Rate
Authors: Bottrel Marina , Ortiz Isabel , Hidalgo Manuel , Díaz-Jiménez María , Pereira Blasa , Consuegra César , Yousef Mohamed Samy , Dorado Jesús
Published: January 2022

Two prostanglandins (luprostiol, LUP, and dinoprost, DIN) and two ovulation-inducing agents (human Chorionic Gonadotropin, hCG, and deslorelin, DES) were evaluated for luteolysis and estrus induction, and for ovulation induction, respectively, in embryo donor jennies. Twenty-six fertile Andalusian jennies were used. In Experiment 1, jennies (n = 112 cycles) were randomly treated with either LUP or DIN after embryo flushing. In Experiment 2, donors (n = 84 cycles) were randomly treated with either hCG or DES to induce ovulation. No differences were found between prostaglandins for all variables studied (prostaglandin–ovulation interval (POI), interovulatory interval (IOI), embryo recovery rate (ERR), positive flushing rate (PFR) and embryo grade (EG)). The ovulation rate was similar for hCG and DES (60.9% vs. 78.7%). However, the interval to ovulation (ITO) was affected (62.61 ± 7.20 vs. 48.79 ± 2.69 h). None of the other variables studied (ERR, PFR and EG) were affected (p > 0.05), except for embryo quality (p = 0.009). In short, both prostaglandins evaluated are adequate to induce luteolysis and estrus. Both ovulation-inducing agents hastened ovulation, but DES seems to be more effective than hCG. Follicular diameter affected the interval from treatment to ovulation, and high uterine edema was related to low embryo quality.
Integrity of the Intestinal Barrier: The Involvement of Epithelial Cells and Microbiota—A Mutual Relationship
Authors: Gieryńska Małgorzata , Szulc-Dąbrowska Lidia , Struzik Justyna , Mielcarska Matylda Barbara , Gregorczyk-Zboroch Karolina Paulina
Published: January 2022

The gastrointestinal tract, which is constantly exposed to a multitude of stimuli, is considered responsible for maintaining the homeostasis of the host. It is inhabited by billions of microorganisms, the gut microbiota, which form a mutualistic relationship with the host. Although the microbiota is generally recognized as beneficial, at the same time, together with pathogens, they are a permanent threat to the host. Various populations of epithelial cells provide the first line of chemical and physical defense against external factors acting as the interface between luminal microorganisms and immunocompetent cells in lamina propria. In this review, we focus on some essential, innate mechanisms protecting mucosal integrity, thus responsible for maintaining intestine homeostasis. The characteristics of decisive cell populations involved in maintaining the barrier arrangement, based on mucus secretion, formation of intercellular junctions as well as production of antimicrobial peptides, responsible for shaping the gut microbiota, are presented. We emphasize the importance of cross-talk between gut microbiota and epithelial cells as a factor vital for the maintenance of the homeostasis of the GI tract. Finally, we discuss how the imbalance of these regulations leads to the compromised barrier integrity and dysbiosis considered to contribute to inflammatory disorders and metabolic diseases.
Metagenomic Comparisons between Soft and Hard Feces of Plateau Pikas (Ochotona curzoniae)
Authors: Fu Haibo , Li Wenjing
Published: January 2022

The division of hard and soft feces is an effective digestion strategy in the order Lagomorpha. Although previous studies have reported that hard and soft feces differ in morphology and component, the discrepancy in the microbiome remains unclear. This study explored the microbiomes of hard and soft feces in plateau pikas by sequencing the V3 and V4 regions of 16S rDNA. We found that hard feces harbored higher Firmicutes, while soft feces harbored higher Akkermansia. Increased rare bacterial taxa were observed in hard feces compared with soft feces. Moreover, hard and soft feces displayed a greater difference in terms of core operational taxonomy units (OTUs) compared to the total OTUs. The soft feces showed enhancements in all predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) functions, indicating an advancing microbial metabolism compared to hard feces. The significantly upregulated pathways in soft feces were mainly enriched in metabolism of energy and carbohydrate, glycan biosynthesis, cofactors and vitamins, and amino acids—all of which are associated with increased contents of microbial proteins, vitamins, and short-chain fatty acids. Our study reports, for the first time, the differential microbiomes between hard and soft feces of pikas and provides direction for the future studies on cecotrophy.
Shelf-Life Evaluation of Ingredient Combinations and Technologies for Use in Pet Food Formulations
Authors: Wagoner Madison P. , Presume Marc R. , Chilenje Moses E. , Abascal-Ponciano Gerardo A. , Sandoval Jorge L. , Smith Hunter R. , Reyes Tristan M. , Wilborn Barney S. , Dunavant Justin A. , Mason Robert P. , Altom Eric K. , Starkey Charles W. , Sawyer Jason T.
Published: January 2022

Poultry co-product chicken frames (CF) and wooden breast (WB) along with ingredient technology use may bring enhanced value to the pet food industry. Therefore, the current study focused on evaluating CF and WB combinations along with sodium alginate and encapsulated calcium lactate pentahydrate (ALGIN) inclusion within a fresh pet food formulation under simulated shelf-life conditions. Fresh chicken frames (CF) and boneless-skinless wooden breast (WB) were ground and allocated randomly to one of ten treatment combinations with either 0.5 or 1.0% added ALGIN. Ground treatments were placed into a form and fill vacuum package and stored using a reach-in refrigerated case for 21 days. Packages were evaluated for instrumental surface color, lipid oxidation, water activity, and pH on days 1, 3, 7, 14 and 21 of the display. Packages of pet food were lighter, less red, and more yellow (p < 0.05) with increasing percentages of CF regardless of ALGIN inclusion, whereas pH was greater (p < 0.05) and lipid oxidation was less (p < 0.05) with increasing percentage of WB. Water activity increased (p < 0.05) when WB and ALGIN inclusion increased. The current results suggest that the use of ALGIN in a poultry co-product pet food formulation can improve shelf-life characteristics such as surface color and lipid oxidation in fresh pet food.
Comparing Blind and Ultrasound-Guided Retrobulbar Nerve Blocks in Equine Cadavers: The Training Effect
Authors: Hermans Hanneke , Lloyd-Edwards Ralph A. , Ferrão-van Sommeren Aukje J. H. , Tersmette Anne A. , Schouten Jacobine C. M. , Serra Bragança Filipe M. , van Loon Johannes P. A. M.
Published: January 2022

In standing ophthalmic surgery in horses, a retrobulbar nerve block (RNB) is often placed blindly for anesthesia and akinesia. The ultrasound (US)-guided RNB may have fewer complications, but the two techniques have only been compared once in equine cadavers. This study compares the techniques for success and complication rates and analyzes the effect of training on US guidance. Twenty-two equine cadavers were divided into three groups: blind RNBs were performed bilaterally in eight cadavers, US-guided RNBs were performed bilaterally in seven cadavers, and after US-guided training, blind RNBs were performed bilaterally in seven cadavers. All RNBs were performed by the same two inexperienced operators, and a combination of contrast medium (CM; 1.25 mL) and methylene blue dye (1.25 mL) were injected (2.5 mL total volume). Needle positioning in the periorbita and the distance of the CM to the optic foramen were assessed using computerized tomography (CT). Dye spreading was evaluated by dissection. In group 1, 37.5% of the injections were in the optimal central position in the periorbita; in group 2, 75% and in group 3, 71.4%. There was no significant difference between the groups regarding needle position (groups 1 and 2 p = 0.056; groups 1 and 3 p = 0.069, groups 2 and 3 p = 0.8). The mean CM distribution distance was not significantly different between all groups. Group 1 had 18.75% intraocular injections versus 0% in group 2 and 7.1% in group 3 (not significant). US guidance showed no significant increases in accuracy nor decreases in complications. However, the effects on accuracy showed a trend towards significant improvement, and larger scale follow-up studies might show significant training effects on US guidance.
Appetite Stimulant and Anti-Emetic Effect of Mirtazapine Transdermal Ointment in Cats Affected by Lymphoma Following Chemotherapy Administration: A Multi-Centre Retrospective Study
Authors: Ferro Livia , Ciccarelli Stefano , Stanzani Giacomo , Nappi Lisa , Angelini Francesca , Leo Chiara
Published: January 2022

In humans, mirtazapine can prevent chemotherapy-induced nausea and vomiting (CINV) and improve cancer patients’ quality of life (QoL). This drug is being increasingly used as an appetite stimulant in cats. The hypothesis of this retrospective study was that mirtazapine could reduce the incidence of CINV and weight loss in feline patients affected by lymphoma. The objectives were to report the use of mirtazapine transdermal ointment and assess the incidence of gastrointestinal (GI) toxicity and weight loss in cats diagnosed with lymphoma and receiving chemotherapy. Transdermal mirtazapine was topically administered to the inner surface of the pinna (2 mg/cat/daily) for 14 days following chemotherapy administration. Data recorded from 20 patients were collected. Different grades of GI toxicity were shown in 8/20 (40%) patients. Body weight (BW), body condition score (BCS), and muscle condition score (MCS) improved in 12/20 (60%), 6/20 (30%), and 2/20 (10%) cats, respectively. Mirtazapine-induced adverse events (AEs) occurred in 4/20 (20%) cats and did not require mirtazapine discontinuation. Substantial weight loss was not encountered, suggesting that patients had an adequate food intake after chemotherapy administration. Transdermal mirtazapine ointment was considered safe and well tolerated.
Effects of Restricted Feeding on Growth Performance, Intestinal Immunity, and Skeletal Muscle Development in New Zealand Rabbits
Authors: Zhuang Junyi , Zhou Tong , Bai Shaocheng , Zhao Bohao , Wu Xinsheng , Chen Yang
Published: January 2022

This study aimed to explore the effects of different feeding restriction levels on the growth performance, intestinal immunity, and skeletal muscle development of meat rabbits. Additionally, we studied whether complete compensatory growth could be obtained post 2 weeks of restricted feeding, in order to seek a scientific mode of feeding restriction. Each of three groups was exposed to 3 weeks of feeding restriction and 2 weeks of compensatory growth. The 15% feeding restriction showed a negligible effect on the final body-weight of the rabbits (p > 0.05), but significantly reduced the feed-to-weight ratio (p < 0.05); reduced diarrhea and mortality; and increased digestive enzyme activity and antioxidant capacity. However, a 30% feeding-restriction level substantially reduced the growth rate of the rabbits (p < 0.05), impaired skeletal muscle development, and showed no compensatory growth after 2 weeks of nutritional recovery. Additionally, immunoglobulin and antioxidant enzyme synthesis were impaired due to reduced nutritional levels, and levels of pro-inflammatory factors were increased during the compensation period. The IGF1 mRNA expression decreased significantly (p < 0.05), whereas MSTN and FOXO1 expression increased noticeably (p < 0.05). Moreover, protein levels of p-Akt and p-p70 decreased significantly in the 15% feeding restriction group. Overall, the 15% feeding limit unaffected the weight and skeletal muscle development of rabbits, whereas the 30% feeding limit affected the growth and development of skeletal muscle in growing rabbits. The PI3K/Akt signaling pathway is plausibly a mediator of this process.
Phytotoxicity and Accumulation of Antibiotics in Water Lettuce (Pistia stratiotes) and Parrot Feather (Myriophyllum aquaticum) Plants under Hydroponic Culture Conditions
Authors: Park Young-Jae , Son Jae-Gwon
Published: January 2022

The aim of this research was to investigate the accumulation of antibiotics in two kinds of plants. The hydroponic culture solution included a mixture of antibiotics, including three commonly used antibiotics in South Korea: norfloxacin (NOR), sulfamethazine (SMZ), and tetracyclines (TC). None of these antibiotics were detected in the shoots of water lettuce plants, only in the roots. However, in parrot feather plants, antibiotics were detected in both the shoots and the roots, with higher amounts detected in the shoots than in the roots. SMZ and TC were most likely to be detected in the roots and shoots of water lettuce and parrot feather plants, and about one-third of the NOR administered was later detected in the plants. The BCF (bioconcentration factor) of antibiotics ranged from 0.24 to 0.78, while that of NOR was much lower, ranging from 0.24 to 0.38. The SMZ (0.59–0.64) and TET (0.72–0.78) exhibited higher uptake accumulation in the water lettuce tissues compared with the parrot feather plants.
Long-Term Impacts of Weather Conditions on Indoor Radon Concentration Measurements in Switzerland
Authors: Rey Joan Frédéric , Goyette Stéphane , Gandolla Mauro , Palacios Martha , Barazza Fabio , Goyette Pernot Joëlle
Published: January 2022

Radon is a natural and radioactive gas that can accumulate in indoor environments. Indoor radon concentration (IRC) is influenced, among other factors, by meteorology, which is the subject of this paper. Weather parameters impact indoor radon levels and have already been investigated, but rarely in Switzerland. Moreover, there is a strong need for a better understanding of the radon behaviour inside buildings in Switzerland for public health concerns as Switzerland is a radon prone area. Based on long-term, continuous, and hourly radon measurements, radon distributions classified according to different weather event definitions were investigated and then compared at three different study sites in Western Switzerland. Outdoor temperature influences the most indoor radon, and it is globally anti-correlated. Wind influences indoor radon, but it strongly depends on intensity, direction, and building characteristics. Precipitation influences periodically indoor radon levels relatively to their intensity. Atmospheric pressure and relative humidity do not seem to be huge determinants on IRC. Our results are in line with previous findings and provide a vivid example in Western Switzerland. This paper underlines the different influence complexities of radon, and the need to communicate about it within the broader public and with construction professionals, to raise awareness.
The Thermal Environment of Housing and Its Implications for the Health of Older People in South Australia: A Mixed-Methods Study
Authors: Hansen Alana , Williamson Terence , Pisaniello Dino , Bennetts Helen , van Hoof Joost , Arakawa Martins Larissa , Visvanathan Renuka , Zuo Jian , Soebarto Veronica
Published: January 2022

Older people are often over-represented in morbidity and mortality statistics associated with hot and cold weather, despite remaining mostly indoors. The study “Improving thermal environment of housing for older Australians” focused on assessing the relationships between the indoor environment, building characteristics, thermal comfort and perceived health/wellbeing of older South Australians over a study period that included the warmest summer on record. Our findings showed that indoor temperatures in some of the houses reached above 35 °C. With concerns about energy costs, occupants often use adaptive behaviours to achieve thermal comfort instead of using cooling (or heating), although feeling less satisfied with the thermal environment and perceiving health/wellbeing to worsen at above 28 °C (and below 15 °C). Symptoms experienced during hot weather included tiredness, shortness of breath, sleeplessness and dizziness, with coughs and colds, painful joints, shortness of breath and influenza experienced during cold weather. To express the influence of temperature and humidity on perceived health/wellbeing, a Temperature Humidity Health Index (THHI) was developed for this cohort. A health/wellbeing perception of “very good” is achieved between an 18.4 °C and 24.3 °C indoor operative temperature and a 55% relative humidity. The evidence from this research is used to inform guidelines about maintaining home environments to be conducive to the health/wellbeing of older people.