bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathogenetic scenarios of the development of brain ischemia and the main elements of the ischemic cascade
Authors: Shilenko L. A. , Karpov A. A. , Veretennikova E. I. , Shilenko A. A. , Galagudza M. M.
Published: April 2024

Ischemic stroke is one of the leading causes of disability and mortality. The key approaches to the treatment of this pathology are thrombolytic therapy and thromboaspiration. Despite their proven efficacy, the application of these methods is limited by a relatively narrow therapeutic window. There remains a great relevance in the further study of the molecular mechanisms of ischemic- reperfusion brain damage within the framework of the ischemic cascade concept. The impact on the mechanisms of induction and implementation of various types of programmed cell death with the help of pharmacological agents is a promising approach to reduce brain damage in ischemic stroke. This review considers the key processes leading to irreversible damage to neurons and their death. The mechanisms of formation and the role of excitotoxicity, calcium overload, oxidative and nitrosyl stress, mitochondrial dysfunction, as well as the triggering of signaling pathways of apoptosis and aseptic inflammation are considered in detail. The issue of various variants of cell death against the background of these pathological processes is discussed. Along with the consideration of signaling pathways that contribute to the progression of brain damage, special attention is paid to the activation of protective signaling mechanisms during ischemia, which provide an increase in cell resistance to death.
L'alchimie des nuages : étude du Petit éloge de la lumière nature du poète tchadien Nimrod; à la lumière de Rimbaud et de Baudelaire
Authors:   Société d’Etudes Franco-coréennes, SONG Hongjin
Published: April 2024

Dans le recueil des poèmes Petit éloge de la lumière nature, Nimrod, poète né au Tchad, relie l'Afrique et l'Europe dans la tradition poétique des éloges, éclairant à sa manière l'essence de la poésie de Rimbaud et Baudelaire. Après Saint-John Perse et Senghor, Nimrod propose une nouvelle façon de célébrer la nature. Dans son voyage d'exil, les paysages de son enfance sont ravivés avec une sensibilité fremissante et les paysages inconnus de l'Europe découverts à travers les yeux d'un étranger. En voyant le soleil, la lune, les étoiles et en sentant le vent comme Rimbaud, le poète n'oublie jamais que la nature, même dans ses changements, ouvre des horizons universels. Pour Nümod, l'éloge de la “lumière nature” n'est rien d'autre qu'une foi dans le pouvoir d'éveiller les sens, une célébration de la force créatrice qui fait revivre les mondes perdus et une croyance à l'alchimie des nuages comme Baudelaire. Avec cette foi, le poète en exil s'affirme non plus comme “déraciné” mais comme ”l'arbre qui marche”, toujours dans la lumière, toujours avec les nuages.
La Couleur, la langue et l'amour dans La Disparition de la lnngue française d'Assia Djebar: L'Esthétique transfrontière à travers I'effondrement de la structure dichotomique
Authors:   Société d’Etudes Franco-coréennes, JEON Seunghwa
Published: April 2024

Notre article vise à analyser la contribution de la couleur et de la langue à la structure dichotomique dans La Disparition de la langue française d'Assia Djebar, ainsi qu'à examiner l'effondrement de cette structure engendré par la couleur et la langue, qui s'associent avec l'amour pour réaliser l'esthétique transfrontière de l'auteur. L'écrivaine exprime de manière dichotomique la relation entre la France colonisatrice et l'Algérie colonisée. De plus elle utilise la couleur et la langue pour mettre en relief cette structure dichotomique. C'est une stratégie structurelle pour souligner l'écroulement du système dichotomique des deux pays. Enfin, Djebar propose l'idéal transfrontière à travers l'amour des personnages qui connaissent bien la tragédie et la violence du dichtomique
Étude critique des enjeux du discours sur la féminisation de la langue française
Authors:   Société d’Etudes Franco-coréennes, CHOI Ji ln
Published: April 2024

La discussion sur le genre en français est partie du constat d'un déséquilibre socio-culturel des « sexes ». Cela a eu une conséquence concrète : la féminisation des noms de métiers. Un point de vue a émergé qui associe le sexe pris comme symbole de l'identité socio-culturelle au genre des mots, et qui va au-delà de la reconnaissance du genre grammatical en tant qu'élement linguistique pour le lier au produit complexe qui se construit autour du sexe biologique et social. Le langage non sexiste permet de faire reconnaître qu'il s'agit d'une question socio- culturelle plutôt que linguistique et permet d'affirmer que l'égalité entre les genres est un moyen de réaliser l'égalité entre les sexes.
Cette étude présente les enjeux de la féminisation de la langue française. Nous examinerons le discours en faveur de la féminisation, invoquant des enjeux politiques et symboliques, et le discours contre, s'opposant au concept d'un langage non sexiste. Notre discussion vise à observer des faits linguistiques et à examiner s'ils suscitent des arguments valables et appropriés pour ou contre des changements linguistiques.
La triple réécriture chez Michel Tournier: à travers le conte “Le Roi mage Faust”
Authors:   Société d’Etudes Franco-coréennes, KWON Seowon
Published: April 2024

Le roman Gaspard, Melchior et Balthazar, une oeuvre réécrite à partir d'un mythe biblique, paraît en 1980. Trois ans après ce roman, Tournier publie Les Rois Mages. C'est une version réduite du roman initial, une version pour les enfants. Cependant, l'auteur ne se contente pas de ses deux premiers textes. Il réécrit en effet une troisième fois ce mythe des Rois mages sous la forme d'un conte intitulé “Le Roi mage Faust” dans le recueil Le Médianoche amoureux. Cette triple réécriture n'est pourtant pas nouvelle pour Tournier. Bien avant cette série, il a déjà effectué de telles écritures intertexuelles particulièrement intéressantes et complexes. Afin de comprendre cette réécriture à trois niveaux qui lui est propre, nous remontons donc d'abord à sa première occun-ence et examinons ses deux séries de réécritures, celle de ‘Vendredi’, puis celle de ‘Rois mages’. Nous analysons ensuite le conte dans l'intention de clarifier comment et pourquoi l'auteur réécrit sa dernière variante du mythe des Rois mages. Cette étude nous permet enfin de comprendre sa philosophie de la réécriture.
Criminología Verde una Disciplina Emergente. Revisión Bibliométrica (2000-2022).
Authors: Gómez Velasco Nubia , Rincón Báez Lina , Sanabria Higuera Diego
Published: April 2024

La criminología verde se enfoca principalmente en el estudio de los impactos negativos generados hacia un entorno natural. El objetivo de este artículo fue evaluar tendencias de producción científica sobre criminología verde y a través de revisión documental, identificar principales fuentes de contaminación hídrica. La búsqueda se realizó en inglés, en la base Scopus, delimitando el periodo de búsqueda entre los años 2000 a 2022. Se encontraron 405 documentos. Las principales tendencias de la producción científica en el ámbito internacional presentan una tasa de crecimiento promedio anual del 27.1 %, con un incremento de publicaciones en revistas indexadas en diferentes bases de datos de referencias bibliográficas. Esta investigación contribuye al conocimiento de las dinámicas de producción científica sobre criminología verde y sirve de referencia con aportes para el diseño de futuras investigaciones sobre el tema.
Analyzing Labor Costs in The Transport and Storage Sector in European Countries Through Trend and Cluster Analysis:2007-2022
Authors: Yıldırım Metin
Published: April 2024

Trends in the transport and storage industry's labor markets in Europe have significantly impacted changes in labor costs. Technological advancements, increased privatization and deregulation, and changes in global capital flows are among the key reasons that have resulted in significant changes in labor costs in the logistics sector during the last few decades, especially in Europe. The amount of remuneration for logistics personnel and its pace of change over time are essential aspects influencing not just capital investment choices but also country competitiveness. The clusters of countries with similar wage increases over time are also impacted by their economic, social, and ecological problems to a similar extent. The existence of probable trends in labor costs in transport and storage on a country level, as well as cluster analysis over the countries where the trend is found, will aid in revealing significant similarities and contrasts concerning the sector and the countries involved in the research. The Labor Cost Index (LCI), according to NACE Rev. 2 Activity-nominal value, quarterly data released by Eurostat was utilized for this research. Sixty-four quarterly periods have been covered between the first quarter of 2007 and the fourth quarter of 2022. The research was based on data collected from 23 different European countries. The Mann-Kendall trend test and Sen's slope test were used for trend analysis, and the K-means clustering algorithm was used for cluster analysis.
SALON DERGİSİ: ŞEKİL - MUHTEVA ÖZELLİKLERİ VE AÇIKLAMALI DİZİNİ
Authors: Çetinkol Mehmet , Çetinkol Mustafa , Çetinkol Döne
Published: April 2024

Süreli yayınlar, kendi dönemlerine ayna tutmaları açısından kıymetli araçlardır. Gazetelerden farklı olarak derinlikli okuma sunan dergiler; yaşanılan dönemin sosyal, kültürel, sanatsal, siyasi, ekonomik vb. durumlarını yansıtmaları hasebiyle tarihî birer vesika olarak da görülmüştür. Salon dergisi, Ramiz Gökçe’nin sanat hayatının son döneminde katıldığı yayımcılık faaliyetinin bir ürünüdür. Basın dünyasında 1918’den itibaren karikatürleri ile yer alan, eserleri ile inkılapların destekçisi olan Ramiz Gökçe’nin bu dergisi, 1 Kasım 1947 ile 15 Mayıs 1950 tarihleri arasında 62 sayı olarak yayımlanmıştır. İkinci Dünya Savaşı’ndan sonra yeniden şekillenen dünyada yönünü Batı’ya dönen Türkiye’nin sosyo-kültürel değişiminin okunabileceği Salon; Hollywood haberleri, konserler, balolar, tiyatro gruplarının etkinliklerinin haberleştirilmesi ile toplumdaki dönüşüm ve değişimi de gözler önüne serer. Dergi, bu konuları kadın teması üzerinden bayağılaşmadan verir. Salon, modern dünya ve onun merkezindeki kadına yer vermesi kadar Anadolu kadınına, Türk kültür ögelerine de yabancı kalmaz. Topkapı Sarayı, tarihî eserler, yemekler, halılar, el oyaları, müzelerdeki sanat eserleri dergideki yazıların ve röportajların konusu olur. Ayrıca mezkûr dergi, bünyesinde barındırdığı Erken Cumhuriyet Dönemi’nin önde gelen yazar/şair kadrosu ve sayfalarını açtığı amatör şairlerle, muhtevasındaki edebî ürünlerle edebiyat tarihi açısından dikkate şayan bir niteliktedir. Bundan dolayı çalışmada, Salon dergisinin şekil ve muhteva özellikleriyle genel yayın politikası değerlendirilmiş; 1940’lı yıllardaki edebî gelişmeler ortaya konmuş, çalışmanın önemli bir kısmında ise derginin zengin içeriğini yansıtan yazılar dizini verilmiştir. Böylelikle dergiyle ilgili çalışmalar yapacak araştırmacılar için de istifade fırsatı sunulmuştur.
Integração de técnicas de inteligência artificial para modelagem probabilística do estudante em ambientes virtuais de aprendizagem
Authors: Ferreira Hiran N. M. , Araújo Rafael , Dorça Fabiano , Cattelan Renan
Published: April 2024

Práticas pedagógicas apoiadas por recursos computacionais, especialmente aquelas que incorporam técnicas de Inteligência Artificial, podem auxiliar na predição do nível de conhecimento de estudantes em ambientes virtuais de aprendizagem. Nesse contexto, este artigo apresenta uma abordagem híbrida, baseada em Redes Bayesianas e ontologias, para tratar informações sobre o nível de conhecimento e comportamento dos estudantes e, assim, medir seu desempenho. Foi criado um modelo de estudante dinâmico, probabilístico, independente de domínio, extensível e reutilizável. Também foi apresentada uma extensão do modelo para permitir a visualização das capacidades e limitações dos estudantes. Como estudo de caso, o modelo proposto foi integrado a uma plataforma educacional, servindo de base para validação e experimentação da abordagem.
Lifecycle carbon footprints of buildings and sustainability pathways in China
Authors: Zhou Yuekuan , Yu Xiaojun , Zhang Xinyue
Published: April 2024

The Paris Agreement, with a 2 °C temperature increase baseline and a 1.5 °C target temperature increase, imposes challenges to the sustainability of buildings. However, as an end-user or end-of-the-art, the building sector overlaps with other sectors, such as industry and transportation in building materials manufacturing (such as steel, concrete, cement, etc. ) and electricity use (such as building-to-vehicle charging), imposing difficulties in lifecycle carbon footprint quantification in buildings. In this study, the lifecycle carbon footprint of buildings and sustainability pathways in China are provided. Tools and platforms for lifecycle carbon footprint quantification in buildings are reviewed. A global database for lifecycle carbon footprint quantification in buildings is reviewed and compared, together with an analysis of the advantages and disadvantages. Furthermore, decarbonization technologies in the building operation stage are comprehensively reviewed, including the decarbonization potential, technology readiness level (TRL), techno-economic performance, and current technical status. Pathways on carbon neutrality in building sectors in China are provided. The results indicate that inconsistencies in global databases, unclear definitions of lifecycle carbon emissions, and inaccurate models of building energy consumption are the main challenges for accurate lifecycle carbon analysis in buildings. Various carbon neutrality transformation technologies can be classified into ultralow energy building and near zero-energy building technologies and into efficient heat pump and smart metering technologies. Pathways for low-carbon transition in building sectors include building energy saving (330 million tCO2 with 22%), renewable energy supply (299 million tCO2 with 20%), building electrification and power sector decarbonization (450 million tCO2 with 30%), and carbon capture, utilization and storage (CCUS) technology (420 million tCO2 with 28%). These research results can pave the way for upcoming studies on the lifecycle carbon footprint in buildings and sustainability pathways in China.
On the Use of Randomly Selected Partial Charges to Predict Battery State-of-Health
Authors: Byg Vilsen Søren , Stroe Daniel-Ioan
Published: April 2024

As society becomes more reliant on Lithium-ion (Li-ion) batteries, state-of-health (SOH) estimation will need to become more accurate and reliable. Therefore, SOH modelling is in the process of shifting from using simple and continuous charge/discharge profiles, to more dynamic profiles constructed to mimic real operation, when ageing the Li-ion batteries. However, in most cases, when ageing the batteries, the same exact profile is just repeated until the battery reaches its end-of-life. Using data from batteries aged in this fashion to build a model, there is a very real possibility that the model will rely on the built-in repetitiveness of the profile. Therefore, this work will examine the dependence of the performance of a multiple linear regression on the number of charges used to train the model, and their location within the profile used to age the batteries. The investigation shows that it is possible to build models using randomly selected partial charges while still reaching errors as low as 0.5%. Furthermore, it shows that only two randomly sampled partial charges are needed to achieve errors of less than 1%. Lastly, as the number of randomly sampled partial charges used to create the model increases, then the dependence on particular partial charges tends to decrease.
Adaptasi dan Uji Psikometri Alat Ukur Perilaku Ramah Lingkungan Versi Indonesia
Authors: Adishesa Made Syanesti , Rocky  , Suwartono Christiany
Published: April 2024

Perilaku ramah lingkungan telah banyak diteliti di Indonesia. Akan tetapi, dalam kurun waktu lima tahun terakhir belum ada penelitian yang fokus pada pengujian psikometri alat ukur perilaku ramah lingkungan. Tujuan penelitian ini adalah mengadaptasi skala pengukuran perilaku ramah lingkungan serta menguji validitas dan reliabilitas skala tersebut. Sampel penelitian terdiri dari 196 orang dengan rentang usia 18-25 tahun. Versi asli dari alat ukur ini terdiri dari 10 aitem. Versi final dari alat ukur yang telah diadaptasi terdiri dari lima aitem, dan menunjukkan koefisien reliabilitas sebesar 0.78. Alasan eliminasi aitem dalam versi Bahasa Indonesia juga didiskusikan. Selain itu, hasil analisis faktor menghasilkan model satu faktor yang fit dengan data dan mengkonfirmasi bahwa alat ukur ini mengukur satu faktor, yaitu perilaku ramah lingkungan. Dengan demikian, alat ukur Perilaku Ramah Lingkungan versi Bahasa Indonesia disimpulkan sebagai skala yang sahih dan reliabel.
Typomorphic and Technological Characteristics of Quartz of the Mica-Pegmatite Formation on the Example of the Berkutinskaya Vein (South Urals)
Authors: Korekina M.A. , Savichev A.N. , Ankusheva N.N. , Pankrushina E.A. , Artemyev D.A.
Published: April 2024

—Understanding the origin of quartz veins of the mica-pegmatite formation is of great importance for deciphering the emplacement conditions for the of commercially prospective quartz targets. On the example of the Berkutinskaya vein, which is the typical metamorphogenic quartz deposit of the mica-pegmatite formation of the South Urals, we study the source and origin of the mineral-forming fluid, temperatures of quartz formation, the structure of intergranular boundaries, their morphometric feature, the fractal dimension of quartz grains and commercial characteristics of quartz grit. An integrated approach to the study of the typomorphic features of quartz allowed us to assess their impact on the technological characteristics of quartz concentrates. Comparison of data on the content of impurity elements in the Berkutinskaya quartz vein with quartz of the vein No. 175 of OOO Russian Quartz and IOTA-std (Sibelco) showed that the Berkutinskaya quartz vein is promising for commercial development.
Pangenome-based design of strain-specific primers allows the specific monitoring of engraftment in different habitats
Authors: Lebeer Sarah , Eilers Tom , Delanghe Lize , De Boeck Ilke , Rillaer Tim Van , Malderen Joke Van , Bakelants Sam , Beeck Wannes Van , Wittouck Stijn
Published: April 2024

Lactic acid bacteria (LAB), including many well-known beneficial bacteria, have seen a rise in the number of applications with specific strains across various areas, including live biotherapeutic products (LBPs). The most extensively researched strains belong to the Lactobacillaceae. Assessing the survival and persistence of specific strains in different niches is still an important challenge, while selective monitoring techniques are often lacking at strain level. Here, we show a robust pangenome-based approach for detecting singletons, which can be used to develop strain-specific primers. We developed selective and specific primers for six strains across different LAB species. The primers for the widely-used probiotic L. rhamnosus GG and L. plantarum WCFS1 were validated in in vivo studies and showed that these strains can persist in and on other habitats such as the human skin, upper respiratory tract and fermented vegetables. In conclusion, the selection of unique genes derived from the pangenome of a species resulted in a specific and sensitive method based on qPCR to detect and monitor strains in different habitats. This approach can be readily extended to other bacterial strains on other families for any type applications in research and industry.
Aedes aegypti mosquitoes (Diptera: Culicidae) can discriminate by blood sugar: new insights into comorbidity of dengue and diabetes
Authors: bursalı fatma
Published: April 2024

Purpose Aedes aegypti (L.,1762) is a primary vector of arboviral infections like dengue, yellow fever, Zika. Female mosquitoes are influenced by various physical and chemical cues from host when blood feeding, e.g., they find some individuals with certain blood types or certain conditions more attractive than others. This study determined whether Ae. aegypti shows a preference when offered blood from a patient with diabetes mellitus (DM), an endocrine disorder associated with abnormal glucose metabolism, compared to healthy blood from non-DMs
Methods In the dual feeding experiments, forty newly emerged female mosquitoes were provided with two blood feeding systems with blood from a non-diabetic (healthy) and diabetic patient using artificial feeders. Blood from 12 diabetic and 12 non-diabetic patients was matched by ABO blood type (e.g., diabetic type O blood was compared with non-diabetic type O blood). The number of mosquitoes that landed and fed from each membrane was counted every 2 minutes for thirty minutes.
Results Ae. aegypti species significantly preferred for blood from non-diabetic individuals (50-65% among the different blood type groups) compared to blood from diabetic individuals. Using multiplex allele-specific PCR it was also determined that, Ae. aegypti significantly preferred the O blood group regardless of blood sugar level compared to others.
Conclusion Ae. aegypti has less preference for diabetic blood to non-diabetics. Regardless people affected with this condition need to take preventive measures to reduce mosquito bites as they tend to have weaker immune systems and can experience more severe cases of dengue.
Amplicon Barcoding Based Sequencing Reveals Bacterial Diversity From Himalayan Hotspring of Kotli Azad Jammu and Kashmir
Authors: Ishaque Kazima , Shah Asad Hussain , Fariq Anila , Rasheed Sajida Rasheed , Jannat Sammyia , Sharif Sobia
Published: April 2024

Microorganisms are found to exist everywhere including extreme habitats where other forms of life rarely thrive. In this study, culture-independent technique was used to analyze the microbial diversity of water samples from Tata Pani hot spring in district Kotli of Azad Kashmir, Pakistan. Physiochemical analysis was also conducted to study the microbial community composition and diversity of the hot spring in extreme environment. Metagenomic sequencing of five different hot spring samples yielded 875,184 of 16S rRNA sequence reads. The original sequencing output files were submitted in National Centre for Biotechnology Information (NCBI) under the Bioproject ID PRJNA869294 with the accession numbers SRX17083105, SRX17083106, SRX17083107, SRX17083108 and SRX17083109 for 5 hot water samples. Bacterial abundance measures and diversity statistics were assessed using QIIME metagenomics workflow. All the resulting 16S rRNA fragments were then classified into ten bacterial phyla. The highly represented phylum was Proteobacteria (74.68%), followed by Firmicutes (18.58%) and Bacteroidetes (4.25%) respectively. Proteobacteria were detected in a wide temperature range and their relative abundance increased with the decreasing temperature in almost all samples. There were also some unidentified sequences detected at each taxonomic level in all samples. Multivariate analysis indicated that electrical conductivity, temperature and pH are the major environmental factors involved in modelling microbial communities. This is the first study of a high resolution microbial phylogenetic profile of Tatapani hot spring.
Relationship between outdoor walking path preferences and street environment of older adults in a suburban area
Authors: Gu Yangcheng , Wang Xiaorui , Matsushita Daisuke
Published: April 2024

This study aimed to understand the relationship between walking path preferences and street characteristics of older adults who live in suburban areas. Physical inactivity is more prevalent in developed countries because trends in urban design and built environments over the past several decades have led to car-oriented suburban development. Compared to urban areas, suburban areas have different characteristics, including limited traffic, a high rate of aging, low floor area ratios, and low building heights. We measured the spontaneous walking path of 30 older adults for two months using GPS loggers and calculated the Walk Score, Green View Index (GVI), Sky View Ratio (SVR), and the existence of the sidewalk. Participants who walked ≥ 1 km on ≥ 20 days during the 2-month experiment were classified as the active group, whereas others were classified as the inactive group. The results showed that the active group preferred the path with a significantly higher GVI, whereas the inactive group preferred the path with a significantly higher Walk Score. In addition, there was a positive correlation between walking distance and both GVI and SVR. The findings have important implications for urban planners seeking to create a supportive environment that promotes outdoor walking activity.
Educational workshops for health care professionals on compassionate communication following adverse events in health care: a mixed method evaluation
Authors: McQueen Jean , Berry Gillian , Gordon June , Cook Marcia
Published: April 2024

Background: When things go wrong in healthcare patients and families want to be included, they want open honest compassionate communication yet in practice this does not always happen.
Purpose: This study evaluates the impact of compassionate communication skills training offered to 74 health care staff from across NHS Scotland. The training intervention focused on open communication and ‘good’ patient family involvement during adverse event reviews using a person-centred approach.
Methods: The evaluation, completed by two independent reviewers, draws on the 4 levels of the Kirkpatrick model. It aims to determine learners' reaction to the training and its impact on knowledge, confidence, and practice. It draws on both qualitative and quantitative data from participants who completed the training. This includes feedback from course evaluation forms, pre and post measures of knowledge and confidence together with thematic analysis from reflective practice assignments.
Results: Quantitative outcomes indicate participants felt more confident crafting and delivering an apology, communicating, and including patients and families in adverse event reviews. They valued the on-line delivery method which combined both theory and practice. Qualitative analysis of participants' self-reported reflective practice logs suggest the training inspired improved communication, and empathic presence.
Conclusion After completing the training staff felt more confident, and some evidenced self-reported skill enhancement and changes in practice through a more person-centric compassionate approach to involving patients and families in the adverse event review process.
A feasibility study of [18F]F-AraG positron emission tomography (PET) for cardiac imaging – myocardial viability in ischemia-reperfusion injury model
Authors: shrestha uttam , Chae Hee-Don , Fang Qizhi , Lee Randall J. , Packiasamy Juliet , Huynh Lyna , Blecha Joseph , Huynh Tony L. , VanBrocklin Henry F. , Levi Jelena , Seo Youngho
Published: April 2024

Purpose: Myocardial infarction (MI) with subsequent inflammation is one of the most common heart conditions leading to progressive tissue damage. A reliable imaging marker to assess tissue viability after MI would help determine the risks and benefits of any intervention. In this study, we investigate whether a new mitochondria-targeted imaging agent, 18F-labeled 2'-deoxy-2'-18F-fluoro-9-β-d-arabinofuranosylguanine ([18F]F-AraG), a positron emission tomography (PET) agent developed for imaging activated T cells, is suitable for cardiac imaging and to test the myocardial viability after MI.
Procedure: To test whether the myocardial [18F]-F-AraG signal is coming from cardiomyocytes or immune infiltrates, we compared cardiac signal in wild-type (WT) mice with that of T cell deficient Rag1 knockout (Rag1 KO) mice. We assessed the effect of dietary nucleotides on myocardial [18F]F-AraG uptake in normal heart by comparing [18F]F-AraG signals between mice fed with purified diet and those fed with purified diet supplemented with nucleotides. The myocardial viability was investigated in rodent model by imaging rat with [18F]F-AraG and 2-deoxy-2[18F]fluoro-D-glucose ([18F]FDG) before and after MI. All PET signals were quantified in terms of the percent injected dose per cc (%ID/cc). We also explored [18F]FDG signal variability and potential T cell infiltration into fibrotic area in the affected myocardium with H&E analysis.
Results: The difference in %ID/cc for Rag1 KO and WT mice was not significant (p = ns) indicating that the [18F]F-AraG signal in the myocardium was primarily coming from cardiomyocytes. No difference in myocardial uptake was observed between [18F]F-AraG signals in mice fed with purified diet and with purified diet supplemented with nucleotides (p = ns). The [18F]FDG signals showed wider variability at different time points. Noticeable [18F]F-AraG signals were observed in the affected MI regions. There were T cells in the fibrotic area in the H&E analysis, but they did not constitute the predominant infiltrates.
Conclusions: Our preliminary preclinical data show that [18F]F-AraG accumulates in cardiomyocytes indicating that it may be suitable for cardiac imaging and to evaluate the myocardial viability after MI.
Identification of pericyte loss and neutrophils infiltration in Focal cortical dysplasia by Integrated Analysis of Single-Cell and Bulk RNA-Sequencing
Authors: Wang Xin , Wang Weiliang , Xu Fei , Yao Yi , Zhang Xiaobin , Weng Mingxiang , Wang Fengpeng , Gang Baozhi
Published: April 2024

Introduction
Epilepsy is one of the most common serious brain conditions. Focal cortical dysplasia(FCD) is the most common diagnosis in children among patients with drug-resistant epilepsy who require surgical treatment. However, the pathogenic genes and the interaction between different cells of FCD are not clear, especially the immune mechanism of FCD remains nascent. We further analyze FCD data in terms of genes, cellular changes and immune infiltration by integrating bulk and single-cell sequencing data.
Methods
The scRNA-seq dataset GSE201048 and bulk RNA-seq dataset GSE62019 were combined to perform deconvolution to infer the cell composition ratio of samples in bulk RNA-seq of FCD. We used AUCell analysis, GO and KEGG enrichment analysis, cell communication analysis and WGCNA to analyze pericyte function and pericyte cell communication. PPI was used to identify hub genes. GSVA and The Pearson correlation analysis were used to identify relationships between hub genes and pericyte. We used logistic regression to construct a diagnostic model based on hub genes. Finally, we performed immune infiltration analysis.
Results
Pericytes were significantly reduced in FCD. Neutrophils cells were significantly different between the FCD group and the control group ( p < 0.05 ), and the degree of immune infiltration was higher in the FCD group. PPI network analysis identified 5 hub genes related to pericytes. Through LASSO regression analysis, we established a risk score model containing five genes.
Conclusion
This study provides a new perspective to study the molecular mechanism and cell changes in FCD. It was found for the first time that pericytes play an important role in FCD.
Validity of the checkbox method: a new scale for quantification of response cost
Authors: Oda Ryo , Yasutani Hinako
Published: April 2024

In the checkbox method, participants check boxes in a grid of 10 × 10 checkboxes on a web page by pointing the mouse on each box and clicking. Each box is numbered from 1 to 100 and it is only possible to check them in order. This method is useful for quantitatively measuring the extent to which respondents are willing to pay a cost, such as the willingness to help or to express gratitude. It needs to be tested whether the checkbox method of measurement shows similar results to traditional methods such as the Likert scale in contexts not directly related to cost. However, no studies have compared the results of a Likert scale with those of a checkbox scale in such a context. We asked participants to complete both a Likert scale and a checkbox scale of the Big Five personality traits using the TIPI-J and investigated relationships between the scales. Despite the time-consuming nature of the responses, there were statistically significant correlations between the number of boxes checked and the Likert scale scores, which revealed that the checkbox method can measure the Big Five personality traits correctly to some extent.
Evaluation of the effectiveness of student-centered teaching model in medical microbiology education
Authors: Qian Jun , Xu Weizhen , Kao Wenping , Wang Xu , Zhang Qingmeng , Zhuang Min
Published: April 2024

Background: The student-centered teaching model involves reforms in training mode, teaching methods, assessment systems, class roles and learning behaviors, aiming to improve the teaching quality effectively, which has become an inevitable trend for maximizing teaching efficiency. Medical microbiology is the study of the pathogenic microorganisms related to human infectious diseases, playing a crucial role in the training of medical professionals. However, there is a lack of data demonstrating the effectiveness of this teaching modelin the medical microbiology education of undergraduate medical students. This study aims to evaluate it.
Methods: This study focused on undergraduate clinical medicine students at Harbin Medical University from grade 2018 to 2021. The teaching team began implementing a student-centered teaching model and incorporating online content into the class from grade 2019. The effectiveness of the teaching model was evaluated by comparing the usual scores and final exam scores of different grades under different teaching models used Pearson correlation. An anonymous questionnaire survey was conducted after the course in grade 2020 and 2021 to assess students' opinions on the student-centered teaching model.
Results: The final exam scores of students from grade 2019, 2020, and 2021 using the new teaching model (85.29±0.51, 76.84±0.60, 80.88±0.55) were significantly higher than those of grade 2018 under traditional teaching model (75.83±0.55). There was a significant correlation between usual scores and final exam scores(grade 2019 r =0.3936, grade 2020 r =0.2819, grade 2021 r =0.1953, P < 0.05). Results from the questionnaire showed that 61.62% students of grade 2020 (183/297) and 65.90% students of grade 2021 (201/305) believed that the new teaching model helped integrate knowledge and understand the key contents, 60.27% (179/297) and 67.87% (207/305) of the studentsfelt it increased their desire to learn clinical professional courses, 65.66% (195/297) and 71.80% (219/305) of the studentsthought it enhanced problem analysis and solving abilities, 58.59% (174/297) and 62.30% (190/305) of studentsbelieved it stimulated their interest in studying medical microbiology.
Conclusions: The student-centered teaching model significantly improved student learning efficiency in medical microbiology courses, enhanced the development of higher-order skills, and promoted students' awareness of active learning through various assessments.
The Mediating Role of Police Officers' Emotional Self-Regulation Competency in the Association between Perceived Stress and Suicide Tendency
Authors: Tansel Bülent , Padır Mehmet Ali , Demir Volkan
Published: April 2024

Objectives
Suicide tendency is prevalent among police officers, influenced by various psychological factors and challenging work conditions that impact their well-being and mental health. Despite its significance, there remains a scarcity of evidence addressing this critical issue. The current study aimed to examine the mediating role of regulatory emotional self-efficacy in the association between perceived stress and suicide probability.
Method
The data were collected from 855 police officers (85.8% men; mean age = 35.68 ± 8.83) in Turkey. Using a cross-sectional research design, participants were administered the stress subscale of the Depression, Anxiety, and Stress Scale (DASS), the Regulatory Emotional Self-Efficacy Scale, and the Suicide Probability Scale.
Results
The results showed that perceived stress had a significant direct positive impact on suicidal probability and a negative impact on regulatory emotional self-efficacy. The regulatory emotional self-efficacy had a significant direct negative impact on suicidal probability. Furthermore, regulatory emotional self-efficacy played a partial mediating role in the effect of perceived stress on suicidal probability.
Conclusions
Effective use of emotional self-regulation skills by police officers can mitigate the impact of perceived stress and decrease suicidal tendencies.
MRI Radiomics and Biological Correlations for Predicting Axillary Lymph Node Burden in Early-Stage Breast Cancer
Authors: Hong Minping , Xu Maosheng , Fan Sijia , Xu Zeyan , Fang zhen , Ling keng , Lai Penghao , Han Chaokang , Chen Zhonghua , Hou Jie , Liang Yanting , Zhou Changyu , Wang Junyan , Chen Xiaobo , Huang Yanqi
Published: April 2024

Background and aims Preoperative prediction of axillary lymph node (ALN) burden in patients with early-stage breast cancer is pivotal for individualised treatment. This study aimed to develop a MRI radiomics model for evaluating the ALN burden in early-stage breast cancer and to provide biological interpretability to predictions by integrating radiogenomic data.
Methods This study retrospectively analyzed 1211 patients with early-stage breast cancer from four centers, supplemented by data from The Cancer Imaging Archive (TCIA) and Duke University (DUKE). MRI radiomic features were extracted from dynamic contrast-enhanced MRI images and an ALN burden-related radscore was constructed by the backpropagation neural network algorithm. Clinical and combined models were developed, integrating ALN-related clinical variables and radscore. The Kaplan–Meier curve and log-rank test were used to assess the prognostic differences between the predicted high- and low-ALN burden groups in both Center I and DUKE cohorts. Gene set enrichment and immune infiltration analyses based on transcriptomic TCIA and TCIA Breast Cancer dataset were used to investigate the biological significance of the ALN-related radscore.
Results The MRI radiomics model demonstrated an area under the curve of 0.781–0.809 in three validation cohorts. The predicted high-risk population demonstrated a poorer prognosis (log-rank P< 0.05 in both cohorts). Radiogenomic analysis revealed migration pathway upregulation and cell differentiation pathway downregulation in the high radscore groups. Immune infiltration analysis confirmed the ability of radiological features to reflect the heterogeneity of the tumor microenvironment.
Conclusions The MRI radiomics model effectively predicted the ALN burden and prognosis of early-stage breast cancer. Moreover, radiogenomic analysis revealed key cellular and immune patterns associated with the radscore.
A joint analytic framework towards the problem of survival curves crossing and the application in patients with NSCLC
Authors: Han Xinyue , Zhou Dingzi , Yang Shuai , Weng Yang , Zhang Tao
Published: April 2024

Background: When the problem of survival-curve-crossing arises, the traditional proportional hazard (PH) Cox model usually fails to accurately estimate the effect of prognostic factors. This problem has not been completely resolved in the prognostic studies of non-small cell lung cancer. Therefore, a joint analytic framework towards the SCC problem should be constructed for a better understanding of NSCLC prognosis.
Method: The framework includes segmentation, model selection, and evaluation. It presents a clear roadmap on how to choose an appropriate model given different data conditions. The performance of the framework was evaluated through the data of NSCLC patients from SEER database. We compared its goodness-of-fit with that of the PH Cox model by the Akaike Information Criterion and Cox-Snell Residuals. Besides, we also conducted subgroup analyses to enhance the validity of our framework.
Results: There was a crossing point in the survival curves of chemotherapy. The model selected by the joint analytic framework had a better goodness-of-fit performance. In contrast, the non-segmented model failed to find out that radiotherapy shortened the survival time of patients with survival time >14 months. The subgroup analyses showed specific patients who benefited from radiotherapy or chemotherapy.
Conclusion: The joint analytic framework could help clinicians avoid pitfalls such as SCC during the prognosis study of NSCLC. The prognostic effect of radiotherapy and chemotherapy in NSCLC patients with survival time >14 months needs more attention in the future.
First Report of Hermetia Illucens (Linnaeus, 1758), Black Soldier Fly (Diptera, Stratiomyidae) from Iran
Authors: Abbasi Ebrahim
Published: April 2024

Background
The black soldier fly or Hermetia illucens (Linnaeus, 1758) (Diptera: stratiomyidae) is one of the best-known flies all around the world for its nutritional value. This species is originally a new world species and was described in 1758 by the Swedish. They lay their eggs in moist, decaying organic material such as fruits and manure. Larvae are large saprophagous maggots with six larval instars and reach 25–30 mm in length and 6–8 mm in width in the last stages. Adults of Hermetia illucens are black at all body parts except the first abdominal segment, which is translucent, and the feet-like tarsi, which are white. There are several reports of h. illucens worldwide.
Methods
In northwest Iran, specimens were collected from 36°46'36.9"n 48°27'21.1"e in Zanjan Province, where rice, corn, oilseeds, fruits, and potatoes are cultivated. The specimens were collected from fruit waste decomposing in the field as eggs and larvae and transported to the Shiraz University of medical sciences insectarium. They were reared in the insectarium, and the collected specimens' generation was established in the insectarium. All eggs, larva, pupa, and adult stages were analyzed, and photographs were captured by a sc100 model camera mount under microscope. The specimen was identified using valid identification keys.
Results
Totally, 362 flies were collected including Abricta curvicosta, Alobates pennsylvania, Bllatela americana, Carabus coriaceus, Chorthippus albomarginatus, Crocothemis erythaea, Hermetia illucens Lasius flavus, Omocestus viridulus, Orius insidiosus, Ospherantera coerulescens, and Topinoma sessile.
Conclusions
This is the first detection of Hermetia illucens in Iran.
Socio-Economic Challenges Faced by Parents of Children with Cerebral Palsy at Ekiti State University Teaching Hospital, Ado-Ekiti, Ekiti State, Nigeria: A Cross-sectional Study.
Authors: Opeyemi Oladunni , Oyindamola Olaoye Jewel , Opelopejesu Owolabi , Faith Ajani Olumide
Published: April 2024

Background
Cerebral palsy (CP) is a group of neurological disorders that appear in early childhood and permanently affect body movements and muscle coordination. It is caused by a brain injury during development. This neurological disorder in children has an effect on the quality of life, restricts participation in peer activities, and impedes normal growth and development. Caring for and raising children with CP can have an economic burden on parents/caregivers affected families, the healthcare system, and the economy. The economic burden of CP may add to caregivers’/parents’ difficulties in finding the appropriate health services for children with CP.
Methods
This study aims to explore the socio-economic challenges experienced by parents of children with cerebral palsy at Ekiti State University Teaching Hospital in Ado-Ekiti, Ekiti State, Nigeria. The study adopted a descriptive cross-sectional design among 68 participants who are mostly married women and parents of children with cerebral palsy. The data was collected using a self-administered questionnaire, and descriptive data analysis was used to analyze the data. The respondents were aged between 11–20 to 31–40.
Results
Nearly 80% of the respondents indicated that children with cerebral palsy are a burden to them and face difficulties communicating with their children. Furthermore, 78.4% of the respondents indicated that their child's situation affects their mental health. There is no association between monthly income and the perception of children with CP as a burden.
Conclusion
Caring for children with cerebral palsy is challenging and has a significant negative impact on family, social, and financial resources, particularly in resource-poor countries like Nigeria with little or no social support system. The findings from this study have important implications for the practice of public health in Nigeria. The state government needs to provide support systems and take measures to assist parents of children with developmental disabilities. The findings from this study can be translated to the national level for effective program implementation in relation to cerebral palsy support.
RETRACTED: Session Interest Model for CTR Prediction Based on Feature Co-Action Network
Authors: Wang Qianqian , Liu Fang’ai , Zhao Xiaohui , Tan Qiaoqiao
Published: April 2024

The authors have requested that this preprint be removed from Research Square.
Correlation between circulating dephosphorylated uncarboxylated matrix Gla protein and vascular calcification in peritoneal dialysis patients
Authors: Mao Liman , Huang Haole , Zhou Meiyang , Zhou Canxin
Published: April 2024

Objectives
Peritoneal dialysis (PD) patients are prone to develop vascular calcification (VC), which increases the all-cause mortality in PD patients. Dephosphorylated uncarboxylated matrix Gla protein (dp-ucMGP) is the inactive form of matrix Gla protein (MGP), and MGP is considered as a potent inhibitor of VC. This study is to explore the association between serum dp-ucMGP and abdominal aortic calcification (AAC) in PD patients.
Methods
A total 128 PD patients and 120 healthy controls were enrolled into the study. Serum dp-ucMGP was measured by enzyme-linked immunosorbent assay. Lateral plain X-ray films of abdomen were used to evaluate the abdominal aortic calcification (AAC) score (AACS). PD patients were divided into two groups according to the presence or absence of AAC. The relationships between dp-ucMGP levels and AACS were assessed by Spearman analysis and the value of dp-ucMGP in predicting AAC was evaluated by receiver operating characteristic (ROC).
Results
Serum dp-ucMGP in PD patients were significantly higher than controls. And PD patients with AAC had higher serum dp-ucMGP than that of PD patients without AAC. In 128 PD patients, higher dp-ucMGP was associated with greater risk of present AAC (OR = 2.554, 95%CI 1.415–4.609). The area under ROC curve of dp-ucMGP was 0.9227, the corresponding sensitivity was 0.86, and the specificity was 0.92.
Conclusion
Serum dp-ucMGP levels were positively associated with the AACS in PD patients. Higher serum dp-ucMGP level is independently associated with AAC in PD patients.