bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Term Mortality after Transcatheter Edge-to-Edge Mitral Valve Repair Significantly Decreased over the Last Decade: Comparison between Initial and Current Experience from the MiTra Ulm Registry
Authors: Nita Nicoleta , Tadic Marijana , Mörike Johannes , Paukovitsch Michael , Felbel Dominik , Keßler Mirjam , Gröger Matthias , Schneider Leonhard-Moritz , Rottbauer Wolfgang
Published: April 2024

(1) Objective: We aimed to assess whether the candidate profile, the long-term outcomes and the predictors for long-term mortality after transcatheter edge-to-edge mitral valve repair (M-TEER) have changed over the last decade; (2) Methods: Long-term follow-up data (median time of 1202 days) including mortality, MACCE and functional status were available for 677 consecutive patients enrolled in the prospective MiTra Ulm registry from January 2010 to April 2019. The initial 340 patients treated in our institution before January 2016 were compared with the following 337 patients; (3) Results: Patients treated after 2016 showed significantly less ventricular dilatation (left ventricular end-systolic diameter of 43 ± 13 mm vs. 49 ± 16 mm, p < 0.007), lower systolic pulmonary pressures (50 ± 15 mmHg vs. 57 ± 21 mmHg, p = 0.01) and a lower prevalence of severe tricuspid regurgitation (27.2% vs. 47.3%, p < 0.001) at baseline than patients treated before 2016. Compared to the cohort treated before 2016, patients treated afterwards showed a significantly lower all-cause 3-year mortality (29.4% vs. 43.8%, p < 0.001) and lower MACCE (38.6% vs. 54.1%, p < 0.001), without differences for MR etiology. While severe tricuspid regurgitation and NYHA class IV remained independently associated with an increased long-term mortality over the last decade, severe left ventricular dilatation (hazard ratio, HR 2.12, p = 0.047) and severe pulmonary hypertension (HR 2.18, p = 0.047) were predictors of long-term mortality only in patients treated before 2016. (4) Conclusions: The M-TEER candidates are currently treated earlier in the course of disease and benefit significantly in terms of a better long-term survival than patients treated at the beginning of the M-TEER era.
Integrating the PD-L1 Prognostic Biomarker in Non-Muscle Invasive Bladder Cancer in Clinical Practice—A Comprehensive Review on State-of-the-Art Advances and Critical Issues
Authors: Sanguedolce Francesca , Falagario Ugo Giovanni , Zanelli Magda , Palicelli Andrea , Zizzo Maurizio , Busetto Gian Maria , Cormio Angelo , Carrieri Giuseppe , Cormio Luigi
Published: April 2024

Bladder cancer (BC) is one of the most prevalent cancers worldwide. Non-muscle invasive bladder cancer (NMIBC), comprising the majority of initial BC presentations, requires accurate risk stratification for optimal management. This review explores the evolving role of programmed cell death ligand 1 (PD-L1) as a prognostic biomarker in NMIBC, with a particular focus on its implications in the context of Bacillus Calmette-Guérin (BCG) immunotherapy. The literature suggests a potential association between elevated PD-L1 status and adverse outcomes, resistance to BCG treatment, and disease progression. However, conflicting findings and methodological issues highlight the heterogeneity of PD-L1 assessment in NMIBC, probably due to the complex biological mechanisms that regulate the interaction between PD-L1 and the tumor microenvironment. The identification of PD-L1 as a prognostic biomarker provides ground for tailored therapeutic interventions, including immune checkpoint inhibitors (ICIs). Nevertheless, challenges such as intratumoral heterogeneity and technical issues underscore the need for standardized protocols and larger, homogeneous trials. This review contributes to the ongoing debate on the personalized management of NMIBC patients, focusing on the advances and perspectives of incorporating PD-L1 as a biomarker in this setting.
The Fatigue and Altered Cognition Scale among SARS-CoV-2 Survivors: Psychometric Properties and Item Correlations with Depression and Anxiety Symptoms
Authors: Hsiao Yu-Yu , Elliott Timothy R. , Jaramillo Julie , Douglas Megan E. , Powers Mark B. , Warren Ann Marie
Published: April 2024

Background/Objectives: This study examined the psychometric properties of the Fatigue and Altered Cognition Scale (FACs) among adult COVID-19 survivors and its unique ability to assess symptomology not accounted for by measures of depression and anxiety. Methods: COVID-19 survivors completed an online survey that included the FACs, a measure of brain fog and central fatigue with 20 items rated on a digital–analog scale. Useable data from 559 participants were analyzed to test the two-factor structure of the FACs, test for measurement invariance by sex and device was used to complete the survey (hand-held, computer), and item correlations with symptoms of depression and anxiety were examined. Results: The two-factor structure of the FACs replicated, supporting the separate assessments of brain fog and fatigue, χ2(164) = 1028.363, p < 0.001, CFI = 0.934, TLI = 0.923, RMSEA = 0.097, SRMR = 0.053. The FACs exhibited invariance at the scalar level, indicating item and factor integrity regardless of sex and device type. Using a correlation > 0.70 as a criterion (i.e., indicating more than 50% shared variance between two items), items on the FACs (assessing fatigue and lack of energy) were highly correlated with feeling tired or having little energy on the depression measure. No other items correlated with any anxiety symptom larger than 0.70. Conclusions: The FACs appears to be a psychometrically sound and efficient measure for use with COVID-19 survivors, assessing symptoms of brain fog and central fatigue that are not attributable to symptoms assessed by established measures of depression and anxiety.
Ustekinumab or Vedolizumab after Failure of Anti-TNF Agents in Crohn’s Disease: A Review of Comparative Effectiveness Studies
Authors: Sharip Mohmmed , Nishad Nilanga , Pillay Lushen , Goordyal Nilkantsingh , Goerge Samuel , Subramanian Sreedhar
Published: April 2024

Background: Anti-tumour necrosis factor (TNF) agents are effective in Crohn’s disease (CD), but some patients lose responsiveness and require alternative biologic therapy. Until recently, ustekinumab and vedolizumab were the only other biological agents approved for use in CD. There are no randomised trials which compare the efficacy of these two agents in patients with anti-TNF refractory disease, but several retrospective cohort studies have compared their effectiveness in this setting. Aim: To review the effectiveness of ustekinumab and vedolizumab in anti-TNF refractory patients with CD. Methods: We included studies that compared the effectiveness of ustekinumab and vedolizumab in treating patients with anti-TNF refractory CD. We recorded the sample size, primary and secondary outcome measures and whether the studies employed adjustments for appropriate confounders. Results: Fourteen studies were included with a total sample size of 5651, of whom 2181 (38.6%) were treated with vedolizumab and the rest were treated with ustekinumab (61.4%). Of the fourteen studies included, eight found ustekinumab to be more effective in achieving clinical remission/steroid-free remission in the induction phase or during maintenance therapy (at least 1-year post-treatment) or that treatment persistence rates with ustekinumab were higher than with vedolizumab. Only one study reported vedolizumab to be superior during the maintenance phase in terms of clinical remission or treatment persistence rates. Biochemical outcomes were reported in five studies, two of which showed superiority for ustekinumab at 14 weeks and the other at 52 weeks. Only two studies reported endoscopic and/or radiologic outcomes; of these, one study showed ustekinumab to be significantly better at achieving endoscopic and radiologic responses. Adverse outcomes were broadly comparable, barring a single study which reported a lower hospitalisation rate for severe infection with ustekinumab. Conclusions: Most studies found ustekinumab to be more effective or non-inferior to vedolizumab in treating patients with anti-TNF refractory CD. Although many studies adjusted appropriately for confounders, the possibility of residual confounding remains and further data from prospective studies are warranted to confirm these findings. Further studies are required to compare these two therapies to other emerging therapies, such as Janus-kinase inhibitors.
Comparison between Invasive Intervention and Conservative Treatment in Patients with In-Hospital Myocardial Infarctions: Results from the Regional Myocardial Infarction Registry of Saxony-Anhalt (RHESA) Study
Authors: Assaf Mohamad , Costa Daniela , Efremov Ljupcho , Holland Karen , Mikolajczyk Rafael
Published: April 2024

Background/Objectives: In-hospital myocardial infarctions (AMIs) are less often treated with invasive intervention, compared to out-of-hospital AMIs. We aimed to identify the determinants of invasive intervention in patients with in-hospital AMIs and assess its association with mortality, compared to conservative treatment. Methods: This was a cross-sectional study of in-hospital AMIs in The Regional Myocardial Infarction Registry of Saxony-Anhalt. Patients’ characteristics and outcomes were compared based on the treatment strategy (invasive intervention vs. conservative treatment). Logistic regression was performed to assess the determinants of invasive intervention (vs. conservative treatment) and its association with 30-day mortality. Results: Nearly 67% of the patients (259/386) received invasive intervention, and the rest were treated conservatively. Those who were treated with an invasive intervention were younger and had a lower proportion of chronic heart failure than those treated conservatively. Age > 75 years compared to younger patients, pre-existing heart failure, and higher heart rate upon presentation were associated with lower odds of receiving invasive intervention. Hypertension (OR = 2.86, 95% CI [1.45–5.62]) and STEMI vs. NSTEMI (1.96, [1.10–3.68]) were associated with higher odds of invasive intervention. The adjusted odds of 30-day mortality were lower with invasive intervention compared to conservative treatment (0.25, [0.10–0.67]). Conclusions: One-third of the patients with in-hospital AMIs received conservative treatment. Younger age, absence of heart failure, lower heart rate, hypertension, and STEMI were determinants of invasive intervention usage. Invasive intervention had lower odds of 30-day mortality, but longitudinal studies are still needed to assess the efficacy of conservative vs. invasive strategies in in-hospital AMIs.
Functionalization of PCL-Based Fiber Scaffolds with Different Sources of Calcium and Phosphate and Odontogenic Potential on Human Dental Pulp Cells
Authors: Anselmi Caroline , Mendes Soares Igor Paulino , Mota Rafaella Lara Maia , Leite Maria Luísa , Ribeiro Rafael Antonio de Oliveira , Fernandes Lídia de Oliveira , Bottino Marco C. , de Souza Costa Carlos Alberto , Hebling Josimeri
Published: April 2024

This study investigated the incorporation of sources of calcium, phosphate, or both into electrospun scaffolds and evaluated their bioactivity on human dental pulp cells (HDPCs). Additionally, scaffolds incorporated with calcium hydroxide (CH) were characterized for degradation, calcium release, and odontogenic differentiation by HDPCs. Polycaprolactone (PCL) was electrospun with or without 0.5% w/v of calcium hydroxide (PCL + CH), nano-hydroxyapatite (PCL + nHA), or β-glycerophosphate (PCL + βGP). SEM/EDS analysis confirmed fibrillar morphology and particle incorporation. HDPCs were cultured on the scaffolds to assess cell viability, adhesion, spreading, and mineralized matrix formation. PCL + CH was also evaluated for gene expression of odontogenic markers (RT-qPCR). Data were submitted to ANOVA and Student’s t-test (α = 5%). Added CH increased fiber diameter and interfibrillar spacing, whereas βGP decreased both. PCL + CH and PCL + nHA improved HDPC viability, adhesion, and proliferation. Mineralization was increased eightfold with PCL + CH. Scaffolds containing CH gradually degraded over six months, with calcium release within the first 140 days. CH incorporation upregulated DSPP and DMP1 expression after 7 and 14 days. In conclusion, CH- and nHA-laden PCL fiber scaffolds were cytocompatible and promoted HDPC adhesion, proliferation, and mineralized matrix deposition. PCL + CH scaffolds exhibit a slow degradation profile, providing sustained calcium release and stimulating HDPCs to upregulate odontogenesis marker genes.
Ecology and Phenology of the Subtidal Brown Alga Sargassum furcatum (Ochrophyta, Fucales), a Likely Non-Indigenous Species from the Mediterranean Sea
Authors: Marletta Giuliana , Lombardo Andrea , Serio Donatella
Published: April 2024

This study provides new insights regarding the ecology and phenology of the likely non-indigenous canopy-forming species Sargassum furcatum on the central-eastern coast of Sicily (Italy, Central Mediterranean). This species was described for St. Thomas (Virgin Islands), and it was reported for the first time in the Mediterranean in 1995 in the Chafarinas islands (Spain, western Mediterranean). After the first report, this species was found in 2021 in several sites located along the eastern coast of Sicily (Italy), along the French Mediterranean coastline, in Corsica, and recently also in the Aeolian Islands (Italy). No phenological studies on this species have been ever carried out in the Mediterranean Sea. To conduct this study, a visual census activity was performed in three sites along the Ionian coast of Sicily throughout 2023, during which the length of the main axis of S. furcatum specimens was measured and the frequency of findings of the species during the year was noted. In this study, it was observed that S. furcatum shows a wide adaptability in terms of range of depth, temperature, light exposure, and type of substrate. Since the distribution of this species is mostly centralized in the western Atlantic Ocean, it is likely that S. furcatum entered the Mediterranean through the Gibraltar Strait. Consequently, the entrance of this species in this Basin could be further proof of the ongoing seawater warming and tropicalization of Mediterranean waters. From this point of view, it is important to keep monitoring the dynamics of S. furcatum in the Mediterranean Sea in order to understand its putative impacts on autochthonous communities.
Study on Dynamic Evolution and Erosion Characteristics of Cavitation Clouds in Submerged Cavitating Water Jets
Authors: Cui Yanyu , Zhao Manjun , Ding Qingmiao , Cheng Bin
Published: April 2024

The dynamic evolution behavior of submerged water jet cavitation clouds was studied by combining experiments and simulation. The formation, development, shedding, and collapsing process of a void cloud was analyzed by high-speed camera technology, and the influence of jet pressure was studied. Cavitation water jet erosion experiments were carried out on AL6061 specimens with standard cylindrical nozzles, and the correlation between cavitation cloud evolution and material erosion was studied by surface analysis. The results showed that the evolution of a cavitation cloud has obvious periodicity, that one period is about 0.8 ms, and its action region can be divided according to the attenuation rate of the jet velocity of the nozzle axis. The attenuation rate of the jet velocity at the nozzle axis in the central jet action zone is less than or equal to 82.5%, in the mixed action zone greater than 82.5% and less than 96%, and in the cavitation action zone greater than or equal to 96%. The erosion damage characteristics in different regions of the mixed action zone are significantly different.
BiodiverseCity St. Louis—An Initiative of the Missouri Botanical Garden
Authors: Ponzi Jean , Abney Glenda , Albrecht Matthew A. , Doherty Sean , Hart Robbie , Joyce Allison , Karimi Nisa , Mckelvey Daria , Saxton Mike , Sieradzki Jen
Published: April 2024

Botanical gardens are addressing urgent biodiversity issues through plant-based capacities including botanical research and data-sharing, conservation horticulture, ecological restoration, seed banking, and more. The Missouri Botanical Garden initiative BiodiverseCity St. Louis, led by the Garden’s sustainability division, adds broad community engagement to this mix. This work includes public and professional education, the demonstration and promotion of ecological landscaping and Green Infrastructure practices, citizen science programs, and coordinating communications for a regional network of partner organizations focused on biodiversity. Diverse activity engages businesses, local governments, elementary and secondary (K-12) schools, colleges, and community groups. Community biodiversity work at the Garden is informed by an institutional core of scientific rigor, provides opportunity for internal collaborations, and aligns with global strategies for plant conservation—to ground impactful local work. Missouri Botanical Garden’s experience offers a model for public gardens: leveraging modes of community engagement, in concert with diverse institutional strengths, to address biodiversity needs.
Wafer-Scale Periodic Poling of Thin-Film Lithium Niobate
Authors: Chen Mengwen , Wang Chenyu , Tian Xiao-Hui , Tang Jie , Gu Xiaowen , Qian Guang , Jia Kunpeng , Liu Hua-Ying , Yan Zhong , Ye Zhilin , Yin Zhijun , Zhu Shi-Ning , Xie Zhenda
Published: April 2024

Periodically poled lithium niobate on insulator (PPLNOI) offers an admirably promising platform for the advancement of nonlinear photonic integrated circuits (PICs). In this context, domain inversion engineering emerges as a key process to achieve efficient nonlinear conversion. However, periodic poling processing of thin-film lithium niobate has only been realized on the chip level, which significantly limits its applications in large-scale nonlinear photonic systems that necessitate the integration of multiple nonlinear components on a single chip with uniform performances. Here, we demonstrate a wafer-scale periodic poling technique on a 4-inch LNOI wafer with high fidelity. The reversal lengths span from 0.5 to 10.17 mm, encompassing an area of ~1 cm2 with periods ranging from 4.38 to 5.51 μm. Efficient poling was achieved with a single manipulation, benefiting from the targeted grouped electrode pads and adaptable comb line widths in our experiment. As a result, domain inversion is ultimately implemented across the entire wafer with a 100% success rate and 98% high-quality rate on average, showcasing high throughput and stability, which is fundamentally scalable and highly cost-effective in contrast to traditional size-restricted chiplet-level poling. Our study holds significant promise to dramatically promote ultra-high performance to a broad spectrum of applications, including optical communications, photonic neural networks, and quantum photonics.
Mechanical Properties of the AlCu4Mg1 Alloy Joint Manufactured by Underwater Friction Stir Welding
Authors: Szachogłuchowicz Ireneusz , Śnieżek Lucjan , Wójcik Adam
Published: April 2024

The manuscript presents the results of butt joining of 3-millimeter-thick AlCu4Mg1 alloy sheets using the FSW (friction stir welding) and UWFSW (underwater friction stir welding) methods. The aim of the research is to verify the influence of the water environment on the FSW friction welding process. The article checked three sets of joint parameters. The parameters differed in tool rotation speed and feed rate. The same sets of parameters were used for the FSW and UWFSW fusion techniques. With the supplied devices, metallographic sections are cross-sectioned, and the power supplies are subjected to a light microscope. Microhardness tests and the influence of the heat-affected zone were carried out. A monotonic test was performed. A monotonic test is available, extended with a visual correlation test. The obtained cracked fracture surfaces were examined using a scanning microscope. An analysis of the microfractographic cracking process was carried out. The obtained results did not show any improvement in the strength properties of the obtained joints made using the UWFSW technique when using a scanning microscope.
Study on Compressibility According to Mixing Ratio and Milling Time of Fe-6.5wt.%Si
Authors: Kim Jaemin , Lee Seonbong
Published: April 2024

Recently, researchers have focused on improving motor performance and efficiency. To manufacture motors with performance and efficiency higher than those of motors manufactured through the additive process, compressibility verification through the parameter control of soft magnetic composites (SMCs) is essential. To this end, this study aims to select suitable powders for manufacturing high-performance, high-efficiency motors by exploring powder mixing ratios and milling times. Through physical property tests, the optimal mixing ratio is predicted using the Multi-Particle Finite Element Method (MPFEM) and powder compression molding analysis, and compressibility is predicted in view of the influence of a change in particle size as a function of the powder mixing ratio and milling time. In addition, based on the result of a comparative analysis of density through experiments and an analysis of internal defects through SEM, a 50:50 mixing ratio of hybrid atomizing and gas atomizing powders milled for 3 h provided the best compressibility. Therefore, the use of SMC cores fabricated using powder compression molding is expected to improve motor performance and efficiency.
Study of Dynamic Failure Behavior of a Type of PC/ABS Composite
Authors: Zhou Jiayu , Xia Zhaodong , Ma Dongfang , Wang Huanran
Published: April 2024

PC/ABS composites are commonly used in airbag covers. In this paper, uniaxial tensile experiments of a PC/ABS composite at different temperatures and strain rates were conducted. The results showed that the temperature and loading rate affect the mechanical properties of the PC/ABS composite. As the temperature increases, the yield stress decreases and the strain at the moment of fracture increases, but the strain rate at the same temperature has a relatively small effect on the mechanical properties, which are similar to ductile materials. The experimental results were applied to the Abaqus model which considered thermal effects and the exact Johnson–Cook constitutive parameters were calculated by applying the inverse method. Based on the constitutive model and the failure analysis findings acquired by DIC, the uniaxial tensile test at the room temperature and varied strain rates were simulated and compared to the test results, which accurately reproduced the test process. The experiment on target plate intrusion of the PC/ABS composite was designed, and a finite-element model was established to simulate the experimental process. The results were compared with the experiments, which showed that the constitutive and the failure fracture strains were valid.
An Investigation of the Efficient–Precise Continuous Electrochemical Grinding Process of Ti–6Al–4V
Authors: Yang Guangbin , Ming Pingmei , Niu Shen , Qin Ge , Liu Huan , Li Dongdong , Zhang Anchao
Published: April 2024

Titanium alloys have many excellent characteristics, and they are widely used in aerospace, biomedicine, and precision engineering. Meanwhile, titanium alloys are difficult to machine and passivate readily. Electrochemical grinding (ECG) is an ideal technology for the efficient–precise machining of titanium alloys. In the ECG process of titanium alloys, the common approach of applying high voltage and active electrolytes to achieve high efficiency of material removal will lead to serious stray corrosion, and the time utilized for the subsequent finishing will be extended greatly. Therefore, the application of ECG in the field of high efficiency and precision machining of titanium alloys is limited. In order to address the aforementioned issues, the present study proposed an efficient–precise continuous ECG (E-P-C-ECG) process for Ti–6Al–4V applying high-pulsed voltage with an optimized duty cycle and low DC voltage in the efficient ECG stage and precise ECG stage, respectively, without changing the grinding wheel. According to the result of the passivation properties tests, the ideal electrolyte was selected. Optimization of the process parameters was implemented experimentally to improve the processing efficiency and precision of ECG of Ti–6Al–4V. Utilizing the process advantages of the proposed process, a thin-walled structure of Ti–6Al–4V was obtained with high efficiency and precision. Compared to the conventional mechanical grinding process, the compressive residual stress of the machined surface and the processing time were reduced by 90.5% and 63.3% respectively, and both the surface roughness and tool wear were obviously improved.
Experimental Investigation of Load-Bearing Capacity in EN AW-2024-T3 Aluminum Alloy Sheets Strengthened by SPIF-Fabricated Stiffening Rib
Authors: Khalaf Hassanein I. , Al-Sabur Raheem , Kubit Andrzej , Święch Łukasz , Żaba Krzysztof , Novák Vit
Published: April 2024

The aluminum strength-to-weight ratio has become a highly significant factor in industrial applications. Placing stiffening ribs along the surface can significantly improve the panel’s resistance to bending and compression in aluminum alloys. This study used single-point incremental forming (SPIF) to fabricate stiffening ribs for 1 mm and 3 mm thick aluminum alloy EN AW-2024-T3 sheets. A universal compression machine was used to investigate sheet deformation. The resulting deformation was examined using non-contact digital image correlation (DIC) based on several high-resolution cameras. The results showed that deformation progressively escalated from the edges toward the center, and the highest buckling values were confined within the non-strengthened area. Specimens with a larger thickness (3 mm) showed better effectiveness against buckling and bending for each applied load: 8 kN or 10 kN. Additionally, the displacement from the sheet surface decreased by 60% for sheets 3 mm thick and by half for sheets 1 mm thick, which indicated that thicker sheets could resist deformation better.
Correction: Chen et al. Evolution of Microstructure in Welding Heat-Affected Zone of G115 Steel with the Different Content of Boron. Materials 2022, 15, 2053
Authors: Chen Zhongyi , Kou Dongxu , Chen Zhengzong , Yang Fan , Ma Yonglin , Li Yiming
Published: April 2024

In the original publication [...]
Electrospun Nanofibers Loaded with Marigold Extract Based on PVP/HPβCD and PCL/PVP Scaffolds for Wound Healing Applications
Authors: Paczkowska-Walendowska Magdalena , Rosiak Natalia , Plech Tomasz , Karpiński Tomasz M. , Miklaszewski Andrzej , Witkowska Katarzyna , Jaskólski Maciej , Erdem Cansu , Cielecka-Piontek Judyta
Published: April 2024

Marigold flower is a traditionally used plant material topically applied on the skin due to its anti-inflammatory properties and antibacterial activity. This potential of action justifies the implementation of marigold extract in nanofiber scaffolds based on poly-vinylpyrrolidone/hydroxypropyl-β-cyclodextrin (PVP/HPβCD) and polycaprolactone/polyvinylpyrrolidone (PCL/PVP) obtained by electrospinning for wound treatment. Using SEM, the morphology of electrospun scaffolds showed a fiber diameter in the range of 298–527 nm, with a uniform and bead-free appearance. ATR-FTIR spectroscopy confirmed the presence of marigold extracts in nanofibrous scaffolds. The composition of the nanofibers can control the release; in the case of PVP/HPβCD, immediate release of 80% of chlorogenic acid (an analytical and functional marker for marigold extract) was achieved within 30 min, while in the case of PCL/PVP, the controlled release was achieved within 24 h (70% of chlorogenic acid). All systems showed weak antibacterial activity against skin and wound-infecting bacteria Staphylococcus aureus (MIC 100 mg/mL), and Pseudomonas aeruginosa (MIC 200 mg/mL) and yeasts Candida albicans (MIC 100 mg/mL). Analysis of the effect of different scaffold compositions of the obtained electrofibers showed that those based on PCL/PVP had better wound healing potential. The scratch was closed after 36 h, compared to the 48 h required for PVP/HPβCD. Overall, the study shows that scaffolds of PCL/PVP nanofibers loaded with classic marigold extract have the best potential as wound dressing materials because of their ability to selectively modulate inflammation (via inhibition of hyaluronidase enzyme) and supportive antimicrobial properties, thereby aiding in the early stages of wound healing and repair.
Utilization of Plant Oils for Sustainable Polyurethane Adhesives: A Review
Authors: Ciastowicz Żaneta , Pamuła Renata , Białowiec Andrzej
Published: April 2024

The utilization of plant oils as a renewable resource for the production of polyurethane adhesives presents a promising way to improve sustainability and reduce environmental impact. This review explores the potential of various vegetable oils, including waste oils, in the synthesis of polyurethanes as an alternative to conventional petroleum-based raw materials. The investigation highlights the environmental challenges associated with conventional polyurethane production and highlights the benefits of switching to bio-renewable oils. By examining the feasibility and potential applications of vegetable oil-based polyurethanes, this study emphasizes the importance of further research and development in this area to realize the full potential of sustainable polyurethane adhesives. Further research and development in this area are key to overcoming the challenges and realizing the full potential of plant-oil-based polyurethanes in various industrial applications.
Properties of Geopolymer Mixtures Incorporating Recycled Ceramic Fines
Authors: Kalinowska-Wichrowska Katarzyna , Pawluczuk Edyta , Chyliński Filip , Chai Hwa Kian , Joka Yildiz Magdalena , Chuczun Aleksandra , Łuniewski Stanisław
Published: April 2024

This research aimed to optimize the production conditions for geopolymer matrices by investigating the combination of heat curing conditions and the incorporation of recycled ceramic fines (CFs) as a partial replacement material for fly ash (FA). The obtained physical and mechanical properties of the composites confirmed the positive impact resulting from increasing the curing temperature from 65 °C to 85 °C and using CFs in the amount of 37.5% as a replacement for FA. The results were from laboratory tests performed to evaluate compressive strength, bending strength, bulk density, and water absorption of the geopolymer mixes. In addition, microscopic observations and porosity assessment were also performed, which confirmed that a further increase in the replacement of FA by CFs causes an increase in the porosity of the mixes and, thus, a decrease in all the assessed properties that are relevant to their practical use.
Effects of Lithium Carbonate and Superplasticizer on Ultra-Early Strength of Alite-Ye’elimite-Belite-Ferrite Cement
Authors: Du Peng , Sun Hao , Lu Xiaolei , Huang Yongbo , Cheng Xin
Published: April 2024

Alite-ye’elimite-belite-ferrite cement (AYBFC) integrates the advantages of calcium sulfoaluminate cement and Portland cement, but its ultra-early strength needs to be further improved when applied to rush repair and construction works. In this study, the ultra-early strength of AYBFC was improved using lithium carbonate (Li2CO3) and superplasticizer. The results showed that an appropriate amount of Li2CO3 could significantly improve the ultra-early strength of AYBFC, since it was capable of promoting the hydration reaction of AYBFC. After polycarboxylate superplasticizer was doped on this basis, the ultra-early compressive strength of AYBFC was further improved. This was because the superplasticizer could markedly enhance the matrix compactness despite its inhibitory effect on the hydration reaction of cement and the generation of hydration products.
Correction: Chen et al. Online Trajectory Optimization Method for Large Attitude Flip Vertical Landing of the Starship-like Vehicle. Mathematics 2023, 11, 288
Authors: Chen Hongbo , Ma Zhenwei , Wang Jinbo , Su Linfeng
Published: April 2024

Additional Notes on Article [...]
Understanding CEAP Classification: Insights from an Italian Survey on Corona Phlebectatica and Recurrent Active Venous Ulcers by Vascular Specialists
Authors: Lessiani Gianfranco , Gazzabin Luca , Cocco Giulio , Corvino Antonio , D’Ardes Damiano , Boccatonda Andrea
Published: April 2024

Background and Objectives: The clinical relevance of “corona phlebectatica” and the management of risk factors for recurrence of venous ulcers in patients with chronic venous disease may be variable based on vascular specialists in different geographical areas of Italy. The aim of the present survey is to evaluate the management of patients with chronic venous disease by vascular specialists in different areas of the national territory. In particular, this involves ascertaining the clinical/prognostic relevance attributed to the presence of the “corona phlebectatica” as well as to the management of risk factors related to recurrence of venous ulcers. Materials and Methods: The web-based survey aimed at vascular medicine specialists with particular interest in venous disease. A questionnaire was developed, based on 12 questions, in relation to clinical assessment, risk factor management, and therapy in patients with chronic venous disease. Results: Almost all of the specialists involved actively participated in the survey, declaring that they personally manage chronic venous disease overall. There was a strong agreement in the prognostic consideration attributed to the presence of “corona phlebectatica” and to the management of risk factors for venous ulcer recurrence, regardless of the different geographical areas of interest. Conclusions: Accordingly with the results of this self-assessment survey, the skills and experience of the specialists involved appear to be of a good standard, both in the clinical evaluation and in the management of the progression of chronic venous disease. However, the need to reach more cultural insights into the correlations between chronic venous disease and risk factors correlated with disease progression emerges. Moreover, there was the need for a greater and tighter overall clinical control of a patient with chronic venous disease, also in relation to the presence of comorbidities.
Study of Galena Ore Powder Sintering and Its Microstructure
Authors: Al-Saqarat Bety S. , Al-Mobydeen Ahmed , Al-Dalahmeh Yousef , AL-Masri Ahmed N. , Altwaiq Abdelmnim M. , Hamadneh Imad , Abu-Afifeh Qusay , Zoubi Mutaz M. , Esaifan Muayad , Moosa Iessa Sabbe , AlShamaileh Ehab
Published: April 2024

Galena is a natural mineral enriched with lead sulfide (PbS). It typically forms in hydrothermal veins associated with igneous rocks and can also occur as a gangue mineral in other ore deposits. PbS is of special importance for scientific research applications due to the possibility of tuning its semiconductor energy gap using nanotechnology in conjunction with powder metallurgy as an easy, controllable production route. In this paper, almost pure PbS was successfully produced starting from a high ratio of PbS phase galena ore. As-received galena lumps were roughly pulverized and milled to produce four particle size ranges of 38, 63, 125, and 250 µm prior to compaction and sintering in a vacuum (pre-flushed with argon gas). SEM coupled with the EDAX analysis unit was employed to investigate the microstructure and chemical composition of the as-received galena and the subsequent products after sintering. The chemical analysis confirmed the high ratio of PbS compound in the as-received galena and sintered products with approximately 85% Pb and 13% S mass ratio. The sintering process of the galena powder was carried out at different values of temperature, time, and compaction pressure. Additionally, the effect of length to diameter ratio of compacted and sintered samples was investigated. XRD analysis confirmed the existence of the PbS phase in the as-received and sintered samples at 700 °C with approximately 98 wt.%, as well as a new phase that is formed at 800 °C with a lower percentage. The micro-hardness of the as-received and sintered samples was measured and compared with the as-received galena ore. The results showed a significant reduction in the hardness of sintered galena powder compared with the bulk as-received galena by 52%. Furthermore, a relative sintered density of 99.3% for the as-received galena density signifies a novel result using powder metallurgy techniques.
Study on the Effect of Solid Solution Treatment on the Bending Fatigue Property of Fe-Mn-Si Shape Memory Alloys
Authors: Niu Haojie , Sun Yubin , Lin Chengxin
Published: April 2024

Fe-Mn-Si shape memory alloys have excellent low-cycle fatigue performance and broad application prospects in the field of civil engineering and construction. It is necessary to conduct comprehensive and in-depth research on the mechanical properties of Fe-Mn-Si shape memory alloys. This study takes the Fe17Mn5Si10Cr5Ni shape memory alloy as the research object. After solid solution treatment at different temperatures and times, the effect of solid solution treatment on the bending fatigue performance of Fe-Mn-Si shape memory alloys was studied using bending cycle tests. The phase composition and fracture morphology of the sample were analyzed. The results showed that solid solution treatment can significantly improve the bending fatigue performance of Fe-Mn-Si shape memory alloys, reaching the optimal value at 850 °C for 1 h. The number of bending cycles until fracture increased by 131% compared to untreated specimens. Stress induction γ → ε martensitic transformation occurred in Fe-Mn-Si shape memory alloy specimens during bending cyclic testing, which is reversible. The fracture area of Fe-Mn-Si shape memory alloy specimens is mainly characterized by ductile fracture, with some areas exhibiting quasi-quasi-cleavage fracture characteristics.
Design and Optimization of MEMS Resonant Pressure Sensors with Wide Range and High Sensitivity Based on BP and NSGA-II
Authors: Lv Mingchen , Li Pinghua , Miao Jiaqi , Qiao Qi , Liang Ruimei , Li Gaolin , Zhuang Xuye
Published: April 2024

With the continuous progress of aerospace, military technology, and marine development, the MEMS resonance pressure sensor puts forward the requirements of not only a wide range but also high sensitivity. However, traditional resonators are hardly compatible with both. In response, we propose a new sensor structure. By arranging the resonant beam and the sensitive diaphragm vertically in space, the new structure improves the rigidity of the diaphragm without changing the thickness of the diaphragm and achieves the purpose of increasing the range without affecting the sensitivity. To find the optimal structural parameters for the sensor sensitivity and range, and to prevent the effects of modal disturbances, we propose a multi-objective optimization design scheme based on the BP and NSGA-II algorithms. The optimization of the structure parameters not only improved the sensitivity but also increased the interference frequency to solve the issue of mode interference. The optimized structure achieves a sensitivity and range of 4.23 Hz/kPa and 1–10 MPa, respectively. Its linear influence factor is 38.07, significantly higher than that of most resonant pressure sensors. The structural and algorithmic optimizations proposed in this paper provide a new method for designing resonant pressure sensors compatible with a wide range and high sensitivity.
3D Artificial Skin Platform for Investigating Pregnancy-Related Skin Pigmentation
Authors: Jeong Uiechan , Yoon Sunhee , Park Sungjin , Jeon Tae-Joon , Kim Sun
Published: April 2024

In this study, we created a 3D Artificial Skin Platform that can be used for the treatment of pigmentation by artificially realizing the skin of pregnant women. For the stable realization of 3D artificial skin, a bilayer hydrogel composed of collagen type I and fibrin was designed and applied to the study to reduce the tension-induced contraction of collagen type I, the extracellular matrix (ECM) of artificial skin, by dynamic culture. Oxygen concentration and 17β-Estradiol (E2) concentration, which are highly related to melanin production, were selected as parameters of the pregnancy environment and applied to cell culture. Oxygen concentration, which is locally reduced in the first trimester (2.5–3%), and E2, which is upregulated in the third trimester, were applied to the cell culture process. We analyzed whether the 3D artificial skin implemented in the 3D Artificial Skin Platform could better represent the tendency of melanin expression in pregnant women than cells cultured under the same conditions in 2D. The expression levels of melanin and melanin-related genes in the 2D cell culture did not show a significant trend that was similar to the melanin expression trend in pregnant women. However, the 3D artificial skin platform showed a significant trend towards a 2-6-fold increase in melanin expression in response to low oxygen concentrations (2.5%) and E2 concentrations (17 ng/mL), which was similar to the trend in pregnant women in vivo. These results suggest that 3D artificial skin cultured on the Artificial Skin Platform has the potential to be used as a substitute for human pregnant skin in various research fields related to the treatment of pigmentation.
A Comparison of Different Protocols for the Extraction of Microbial DNA Inhabiting Synthetic Mars Simulant Soil
Authors: Wang Han , Pijl Agata , Liu Binbin , Wamelink Wieger , Korthals Gerard W. , Costa Ohana Y. A. , Kuramae Eiko E.
Published: April 2024

Compared with typical Earth soil, Martian soil and Mars simulant soils have distinct properties, including pH > 8.0 and high contents of silicates, iron-rich minerals, sulfates, and metal oxides. This unique soil matrix poses a major challenge for extracting microbial DNA. In particular, mineral adsorption and the generation of destructive hydroxyl radicals through cationic redox cycling may interfere with DNA extraction. This study evaluated different protocols for extracting microbial DNA from Mars Global Simulant (MGS-1), a Mars simulant soil. Two commercial kits were tested: the FastDNA SPIN Kit for soil (“MP kit”) and the DNeasy PowerSoil Pro Kit (“PowerSoil kit”). MGS-1 was incubated with living soil for five weeks, and DNA was extracted from aliquots using the kits. After extraction, the DNA was quantified with a NanoDrop spectrophotometer and used as the template for 16S rRNA gene amplicon sequencing and qPCR. The MP kit was the most efficient, yielding approximately four times more DNA than the PowerSoil kit. DNA extracted using the MP kit with 0.5 g soil resulted in 28,642–37,805 16S rRNA gene sequence reads and 30,380–42,070 16S rRNA gene copies, whereas the 16S rRNA gene could not be amplified from DNA extracted using the PowerSoil kit. We suggest that the FastDNA SPIN Kit is the best option for studying microbial communities in Mars simulant soils.
Age-Dependent Differences in Postprandial Bile-Acid Metabolism and the Role of the Gut Microbiome
Authors: Majait Soumia , Meessen Emma C. E. , Davids Mark , Chahid Youssef , Olde Damink Steven W. , Schaap Frank G. , Kemper Ellis Marleen , Nieuwdorp Max , Soeters Maarten R.
Published: April 2024

Ageing changes the impact of nutrition, whereby inflammation has been suggested to play a role in age-related disabilities such as diabetes and cardiovascular disease. The aim of this study was to investigate differences in postprandial bile-acid response and its effect on energy metabolism between young and elderly people. Nine young, healthy men and nine elderly, healthy men underwent a liquid mixed-meal test. Postprandial bile-acid levels, insulin, glucose, GLP-1, C4, FGF19 and lipids were measured. Appetite, body composition, energy expenditure and gut microbiome were also measured. The elderly population showed lower glycine conjugated CDCA and UDCA levels and higher abundances of Ruminiclostridium, Marvinbryantia and Catenibacterium, but lower food intake, decreased fat free mass and increased cholesterol levels. Aging is associated with changes in postprandial bile-acid composition and microbiome, diminished hunger and changes in body composition and lipid levels. Further studies are needed to determine if these changes may contribute to malnutrition and sarcopenia in elderly.
Optimization and Testing of a Commercial Viability PCR Protocol to Detect Escherichia coli in Whole Blood
Authors: Jones Kristi L. , Cunha Federico , Casaro Segundo , Galvão Klibs N.
Published: April 2024

Bacteremia, specifically if progressed to sepsis, poses a time-sensitive threat to human and animal health. Escherichia coli is a main causative agent of sepsis in humans. The objective was to evaluate a propidium monoazide (PMA)-based viability PCR (vPCR) protocol to detect and quantify live E. coli from whole blood. We optimized the protocol by adding a eukaryotic-specific lysis step prior to PMA exposure, then used spiking experiments to determine the lower limit of detection (LOD) and linear range of quantification. We also compared the vPCR quantification method to standard colony count of spiked inoculum. Lastly, we calculated percent viability in spiked samples containing 50% live cells or 0% live cells. The LOD was 102 CFU/mL for samples containing live cells only and samples with mixed live and heat-killed cells. The linear range of quantification was 102 CFU/mL to 108 CFU/mL (R2 of 0.997) in samples containing only live cells and 103 CFU/mL to 108 CFU/mL (R2 of 0.998) in samples containing live plus heat-killed cells. A Bland–Altman analysis showed that vPCR quantification overestimates compared to standard plate count of the spiked inoculum, with an average bias of 1.85 Log10 CFU/mL across the linear range when only live cells were present in the sample and 1.98 Log10 CFU/mL when live plus heat-killed cells were present. Lastly, percent viability calculations showed an average 89.5% viable cells for samples containing 50% live cells and an average 19.3% for samples containing 0% live cells. In summary, this optimized protocol can detect and quantify viable E. coli in blood in the presence of heat-killed cells. Additionally, the data presented here provide the groundwork for further development of vPCR to detect and quantify live bacteria in blood in clinical settings.