bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
Authors: Kerep Robert , Šeba Tino , Borko Valentina , Weitner Tin , Keser Toma , Lauc Gordan , Gabričević Mario
Published: May 2023

Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances. It has been reported that the sialic acid groups that terminate the N–glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein. The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs—clindamycin, diltiazem, lidocaine, and warfarin—was quantitatively evaluated using isothermal titration calorimetry. The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction. The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10−5–10−6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference. Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
Evaluation of Reliquefaction Behavior of Coastal Embankment Due to Successive Earthquakes Based on Shaking Table Tests
Authors: Yoo Mintaek , Kwon Sun Yong
Published: May 2023

Liquefaction caused by long-term cyclic loads in loose saturated soil can lead to ground subsidence and superstructure failures. To address this issue, this study aimed to emulate the liquefaction phenomenon based on a shaking table test while especially focusing on the soil behavior mechanism due to the reliquefaction effect. Liquefaction and reliquefaction behaviors were analyzed by ground conditions where an embankment was located on the coastal ground. Silica sand was used for the experiment for various thickness and liquefiable conditions, and the embankment model was constructed above the model ground. For seismic waves, sine wave excitation was applied, and a total of five excitations (cases) were conducted. When the upper ground layer consisted of a non-liquefiable layer, liquefaction did not occur due to the first excitations but occurred by the third excitation. The results indicated that as the earthquake was applied, the water level in the liquefiable layer increased to the height of the non-liquefiable layer and liquefaction could occur. It was identified that even if liquefaction did not occur for the main earthquake, liquefaction could occur due to aftershocks caused by a rise in the groundwater level due to a series of earthquakes. In a general seismic design code, liquefaction assessment is performed only for soil layers below the groundwater level; however, when successive earthquakes occur, unexpected liquefaction damage could occur. Therefore, to mitigate the earthquake risk of liquefaction for coastal embankments, it is necessary to evaluate the liquefaction by aftershocks even when the groundwater level of the ground layer under an embankment is low.
Secure Rate-Splitting Multiple Access for Maritime Cognitive Radio Network: Power Allocation and UAV’s Location Optimization
Authors: Min Lingtong , Li Jiawei , He Yixin , Wang Weiguang
Published: May 2023

This paper investigates the secure rate-splitting multiple access (RSMA) cooperation for the maritime cognitive unmanned aerial vehicle (UAV) network. Specifically, we first take into account the primary privacy information and the secondary maritime UAV’s quality of service. Then, we formulate an optimization problem to maximize UAV’s transmission rate according to the RSMA decoding principle and primary information security requirements. To solve this non-convex problem, we design a CPFS algorithm to allocate the transmit power and adjust the UAV’s location. In addition, the worst case is analyzed, which is the lower-bound secondary transmission rate. Finally, simulation results indicate that the proposed scheme improves the UAV’s transmission rate compared with the traditional schemes.
Archaeology of the Landscape of Metalworking Sites in Italian Alpine Areas (Orobic Alps) between the Middle Ages and the Modern Era
Authors: de Vingo Paolo
Published: May 2023

The article introduces features of iron-working in the north-western Italian Alpine region (specifically, the Valtellina side of the Bergamesque or Orobic Alps) during the Middle Ages by comparing historical data and archaeological sources. This will help shed light on the organisation of the production process, starting from iron ore mining, proceeding to examine the transformation phases and culminating in the conversion of the ore into ingots or bars to produce tools for agricultural or wood-cutting activities. The article follows two distinct paths, initially presenting the main stages of iron-working in Valtellina until the second half of the eighteenth century, followed by an analysis of the mining complex of Val Venina where an extremely important metal-working site is situated. Two separate mining zones were identified, the first deep underground and the second an opencast working site. Furthermore, a series of rooms made of dry-stone walling that provided accommodation for the miners have been brought to light, as well as mineral deposits and stables for the animals required to carry out the activities described by Melchiorre Gioia in his volume “Statistica del Dipartimento dell’Adda” and indicated in the land registers of the Lombardy-Veneto regions carried out in 1815 and 1863.
Spatiotemporal Patterns in Land Use/Land Cover Observed by Fusion of Multi-Source Fine-Resolution Data in West Africa
Authors: Asenso Barnieh Beatrice , Jia Li , Menenti Massimo , Yu Le , Nyantakyi Emmanuel Kwesi , Kabo-Bah Amos Tiereyangn , Jiang Min , Zhou Jie , Lv Yunzhe , Zeng Yelong , Bennour Ali
Published: May 2023

Land Use/Land Cover (LULC) change is a major global concern and a topic of scientific debate. In West Africa, the key trend among the changes of the past few years is the loss of natural vegetation related to changes in different LULC categories, e.g., water bodies, wetland, and bare soil. However, not all detected changes in these LULC categories are relevant for LULC change management intervention in a resource-constrained continent, as a massive change in the dominant LULC types may be due to errors in the LULC maps. Previous LULC change analysis detected large discrepancies in the existing LULC maps in Africa. Here, we applied an open and synergistic framework to update and improve the existing LULC maps for West Africa at five-year intervals from 1990 to 2020—updating them to a finer spatial resolution of 30 m. Next, we detected spatial–temporal patterns in past and present LULC changes with the intensity analysis framework, focusing on the following periods: 1990–2000, 2000–2010, and 2010–2020. A faster annual rate of overall transition was detected in 1990–2000 and 2010–2020 than in 2000–2010. We observed consistent increases in shrubland and grassland in all of the periods, which confirms the observed re-greening of rangeland in West Africa. By contrast, forestland areas experienced consistent decreases over the entire period, indicating deforestation and degradation. We observed a net loss for cropland in the drought period and net gains in the subsequent periods. The settlement category also gained actively in all periods. Net losses of wetland and bare land categories were also observed in all of the periods. We observed net gains in water bodies in the 1990–2000 period and net losses in the 2010–2020 period. We highlighted the active forestland losses as systematic and issued a clarion call for an intervention. The simultaneous active gross loss and gain intensity of cropland raises food security concerns and should act as an early warning sign to policy makers that the food security of marginal geographic locations is under threat, despite the massive expansion of cropland observed in this study area. Instead of focusing on the dynamics of all the LULC categories that may be irrelevant, the intensity analysis framework was vital in identifying the settlement category relevant for LULC change management intervention in West Africa, as well as a cost-effective LULC change management approach.
Effect of Hollow 304 Stainless Steel Fiber on Corrosion Resistance and Mechanical Properties of Ultra-High Performance Concrete (UHPC)
Authors: Li Tianran , Yan Yulong , Xu Chengying , Han Xiangnan , Liu Yang , Qi Haiquan , Ming Yang
Published: May 2023

This study investigated the effect of hollow 304 stainless-steel fiber on the corrosion resistance and mechanical properties of ultra-high-performance concrete (UHPC), and prepared copper-coated-fiber-reinforced UHPC as the control group. The electrochemical performance of the prepared UHPC was compared with the results of X-ray computed tomography (X-CT). The results reveal that cavitation can improve the distribution of steel fibers in the UHPC. Compared with solid steel fibers, the compressive strength of UHPC with hollow stainless-steel fibers did not exhibit significant change, but the maximum flexural strength increased by 45.2% (2 vol% content, length–diameter ratio of 60). Hollow stainless-steel fiber could better improve the durability of UHPC compared with copper-plated steel fiber, and the gap between the two continued to increase as the durability test progressed. After the dry–wet cycle test, the flexural strength of the copper-coated-fiber-reinforced UHPC was 26 MPa, marking a decrease of 21.9%, while the flexural strength of the UHPC mixed with hollow stainless-steel fibers was 40.1 MPa, marking a decrease of only 5.6%. When the salt spray test had run for seven days, the difference in the flexural strength between the two was 18.4%, but when the test ended (180 days), the difference increased to 34%. The electrochemical performance of the hollow stainless-steel fiber improved, owing to the small carrying capacity of the hollow structure, and more uniform distribution in the UHPC and lower interconnection probability were achieved. According to the AC impedance test results, the charge transfer impedance of the UHPC doped with solid steel fiber is 5.8 KΩ, while that of the UHPC doped with hollow stainless-steel fiber is 8.8 KΩ.
Quantitative Evaluation of the Lymph Node Metastases in the Head and Neck Malignancies Using Diffusion-Weighted Imaging and Apparent Diffusion Coefficient Mapping: A Bicentric Study
Authors: Belfiore Maria Paola , Gallo Luigi , Reginelli Alfonso , Parrella Pasquale Maria , Russo Gaetano Maria , Caliendo Valentina , Fasano Morena , Ciani Giovanni , Zeccolini Raffaele , Liguori Carlo , Nardone Valerio , Cappabianca Salvatore
Published: May 2023

This study aimed to determine if diffusion-weighted imaging (DWI) can differentiate between benign and malignant lymph nodes in patients with head and neck cancer. A total of 55 patients with confirmed head and neck cancer and enlarged neck nodes were enrolled and evaluated by two radiologists using a workstation. Lymph nodes were analyzed using 3D regions of interest (ROIs) placed on T2-weighted images and compared to the corresponding DWI images. This study found that DWI and ADC values can be used to assess metastatic lymph nodes in the neck and that the sensitivity, specificity, and AUC of a narrower ROI for recognizing metastases were greater compared to the ADC value of the whole node. The study also found that the size of the ROI affects ADC values. The results suggest that DWI can accurately predict the status of cervical lymph nodes in patients with head and neck cancer and that it may be useful in diagnosing, determining the stage, developing a treatment plan, and monitoring these patients.
Chaotic States of Transistor-Based Tuned-Collector Oscillator
Authors: Petrzela Jiri
Published: May 2023

This brief paper shows that robust chaotic behavior can be detected within a tuned-collector single-stage transistor-based oscillator. The content of this work also contributes to the problem of chaos localization in simplified mathematical model of standard analog building block. Searching for chaos is performed via numerical optimization routine applied onto the principal schematic of oscillator where generalized bipolar transistor is modelled as a two-port described by impedance as well as admittance matrix. In both cases, the presence of dense chaotic attractor is proved via calculation of the largest Lyapunov exponent, while its structural stability is validated by real measurement, i.e., visualization of captured oscilloscope screenshots.
A Data-Driven Decision-Making Model for Configuring Surgical Trays Based on the Likelihood of Instrument Usages
Authors: Ahmadi Ehsan , Masel Dale T. , Hostetler Seth
Published: May 2023

In order to perform a surgical procedure, substantial numbers of sterile instruments should be readily available to surgeons through the containers referred to as surgical trays and peel packs. After the procedure, all instruments in the opened containers, regardless of whether they have been used or not, must go through the labor-intensive re-sterilization process. Empirical studies have shown that the utilization rate of instruments within trays is very low due to not having optimized tray configurations. Additionally, surgical trays often include instruments that are not likely to be used but are included “just in case”, which imposes an additional cost on hospitals through unnecessary instrument re-sterilization. This study is the first analytical attempt to address the issue of configuring surgical trays based on the likelihood of instrument usage through formulating and solving a probabilistic tray optimization problem (PTOP). The PTOP model can serve as a decision support for surgeons by providing them with the tray’s probability of being used for optimally configured trays and its associated reprocessing costs. The PTOP is constructed upon an integer non-linear programming model. A decomposition-based heuristic and metaheuristic method coupled with two novel local search algorithms are developed to solve the PTOP. The application of this model can be illustrated through a case study. We discuss how hospitals could benefit from our model in reducing the costs associated with opening trays unnecessarily before a procedure. Additionally, we conducted a risk analysis to estimate the level of confidence for the recommended solution.
Antimicrobial Peptides from Photosynthetic Marine Organisms with Potential Application in Aquaculture
Authors: García-Beltrán José María , Arizcun Marta , Chaves-Pozo Elena
Published: May 2023

Aquaculture production is at a record level and is estimated to increase in the coming years. However, this production can be negatively affected by infectious diseases produced by viruses, bacteria, and parasites, causing fish mortality and economic losses. Antimicrobial peptides (AMPs) are small peptides that may be promising candidates to replace antibiotics because they are the first line of defense in animals against a wide variety of pathogens and have no negative effects; they also show additional activities such as antioxidant or immunoregulatory functions, which makes them powerful alternatives for use in aquaculture. Moreover, AMPs are highly available in natural sources and have already been used in the livestock farming and food industries. Photosynthetic marine organisms can survive under all kinds of environmental conditions and under extremely competitive environments thanks to their flexible metabolism. For this reason, these organisms represent a powerful source of bioactive molecules as nutraceuticals and pharmaceuticals, including AMPs. Therefore, in this study we reviewed the present knowledge about AMPs from photosynthetic marine organism sources and analyzed whether they could be suitable for use in aquaculture.
Microalgae from Cold Environments and Their Possible Biotechnological Applications
Authors: Montuori Eleonora , Saggiomo Maria , Lauritano Chiara
Published: May 2023

Cold environments include deep ocean, alpine, and polar areas. Even if the cold conditions are harsh and extreme for certain habitats, various species have been adapted to survive in them. Microalgae are among the most abundant microbial communities which have adapted to live in low light, low temperature, and ice coverage conditions typical of cold environments by activating different stress-responsive strategies. These species have been shown to have bioactivities with possible exploitation capabilities for human applications. Even if they are less explored compared to species living in more accessible sites, various activities have been highlighted, such as antioxidant and anticancer activities. This review is focused on summarizing these bioactivities and discussing the possible exploitation of cold-adapted microalgae. Thanks to the possibility of mass cultivating algae in controlled photobioreactors, eco-sustainable exploitation is in fact possible by sampling a few microalgal cells without impacting the environment.
Comparison of the Effects of Metformin and Thiazolidinediones on Bone Metabolism: A Systematic Review and Meta-Analysis
Authors: Chen Ru-Dong , Yang Cong-Wen , Zhu Qing-Run , Li Yu , Hu Hai-Feng , Wang Da-Chuan , Han Shi-Jie
Published: May 2023

Objectives: Studies have shown that people with diabetes have a high risk of osteoporosis and fractures. The effect of diabetic medications on bone disease cannot be ignored. This meta-analysis aimed to compare the effects of two types of glucose-lowering drugs, metformin and thiazolidinediones (TZD), on bone mineral density and bone metabolism in patients with diabetes mellitus. Methods: This systematic review and meta-analysis were prospectively registered on PROSPERO, and the registration number is CRD42022320884. Embase, PubMed, and Cochrane Library databases were searched to identify clinical trials comparing the effects of metformin and thiazolidinediones on bone metabolism in patients with diabetes. The literature was screened by inclusion and exclusion criteria. Two assessors independently assessed the quality of the identified studies and extracted relevant data. Results: Seven studies involving 1656 patients were finally included. Our results showed that the metformin group had a 2.77% (SMD = 2.77, 95%CI [2.11, 3.43]; p < 0.00001) higher bone mineral density (BMD) than the thiazolidinedione group until 52 weeks; however, between 52 and 76 weeks, the metformin group had a 0.83% (SMD = −0.83, 95%CI: [−3.56, −0.45]; p = 0.01) lower BMD. The C-terminal telopeptide of type I collagen (CTX) and procollagen type I N-terminal propeptide (PINP) were decreased by 18.46% (MD = −18.46, 95%CI: [−27.98, −8.94], p = 0.0001) and 9.94% (MD = −9.94, 95%CI: [−16.92, −2.96], p = 0.005) in the metformin group compared with the TZD group.
Potential of Integrated Component-Driven Material Design to Improve Crash Box Performance
Authors: Jafarzadeh-Aghdam Nima , Sparrer Yannik , Zimmermann Jannik , Mäde Konrad Arno Gregor , Sharma Rahul , Schröder Kai-Uwe , Münstermann Sebastian
Published: May 2023

The crushing of crash boxes is a complex phenomenon characterized by a strong interaction of structural and material properties. Many attempts to improve their energy absorption by adopting one aspect resulted in unacceptable unprogressive bucking. Thus, special considerations are required for crash box optimization including both material and structural effects. In this study, the structural behavior of the crash box is analyzed and a new design approach is introduced to fulfill these requirements. Currently, components, processes, and materials are mainly developed independently. However, to exploit the full potential of modern materials in component design, integrative development work is necessary. Component performance-based requirements and corresponding local material properties must be taken into account concurrently. In this work, a component-driven material design approach is presented, in which local-material-property requirements are derived from component simulations. This new approach is demonstrated by the use case of a quasistatically deformed crash box produced out of steel DP600 for the optimization target “energy absorption”. The finite element simulations were carried out on the crash box, and required material properties for improving the crash box performance were derived based on the simulations. Heat treatment strategies were developed afterwards and experimentally validated to fulfill these requirements. The finite element (FE) simulations based on the experimentally extracted material properties reveal the potential of the component-driven material design approach to improve the crash box performance. The introduced approach enables exploiting the full energy-absorption capacity of the material while ensuring the desired service behavior of the component.
Repeated Low-Level Blast Exposure Alters Urinary and Serum Metabolites
Authors: Sigler Austin , Wu Jiandong , Pfaff Annalise , Adetunji Olajide , Nam Paul , James Donald , Burton Casey , Shi Honglan
Published: May 2023

Repeated exposure to low-level blast overpressures can produce biological changes and clinical sequelae that resemble mild traumatic brain injury (TBI). While recent efforts have revealed several protein biomarkers for axonal injury during repetitive blast exposure, this study aims to explore potential small molecule biomarkers of brain injury during repeated blast exposure. This study evaluated a panel of ten small molecule metabolites involved in neurotransmission, oxidative stress, and energy metabolism in the urine and serum of military personnel (n = 27) conducting breacher training with repeated exposure to low-level blasts. The metabolites were analyzed using HPLC—tandem mass spectrometry, and the Wilcoxon signed-rank test was used for statistical analysis to compare the levels of pre-blast and post-blast exposures. Urinary levels of homovanillic acid (p < 0.0001), linoleic acid (p = 0.0030), glutamate (p = 0.0027), and serum N-acetylaspartic acid (p = 0.0006) were found to be significantly altered following repeated blast exposure. Homovanillic acid concentration decreased continuously with subsequent repeat exposure. These results suggest that repeated low-level blast exposures can produce measurable changes in urine and serum metabolites that may aid in identifying individuals at increased risk of sustaining a TBI. Larger clinical studies are needed to extend the generalizability of these findings.
The Role of Endocan in Cardiometabolic Disorders
Authors: Klisic Aleksandra , Patoulias Dimitrios
Published: May 2023

Discovered two decades ago, endocan still represents an intriguing biomarker related to inflammation. Endocan is a soluble dermatan sulphate proteoglycan secreted by endothelial cells. Its expression is observed in tissues related to the enhanced proliferation, especially hepatocytes, lungs, kidneys, etc. Endocan has been investigated in many cardiometabolic disorders that are tightly connected with inflammation, such as type 2 diabetes mellitus, hypertension, atherosclerotic cardiovascular disease, kidney disease, obesity, polycystic ovary syndrome, metabolic syndrome, non-alcoholic fatty liver disease, etc. In this narrative, comprehensive review of the currently available literature, special attention will be paid to the role of endocan in the broad spectrum of cardiometabolic disorders. Since endocan has emerged as a novel endothelial dysfunction marker, the discovery of potential therapeutic strategies for patients with certain cardiometabolic risk factors would be of great importance to delay or even prevent the onset and progression of related complications, mainly cardiovascular.
Creepage Distance Estimation of Hairpin Stators Using 3D Feature Extraction
Authors: Grambow Niklas , Hinz Lennart , Bonk Christian , Krüger Jörg , Reithmeier Eduard
Published: May 2023

The increasing demand for electric drives challenges conventional powertrain designs and requires new technologies to increase production efficiency. Hairpin stator manufacturing technology enables full automation, and quality control within the process is particularly important for increasing the process capacity, avoiding rejects and for safety-related aspects. Due to the complex, free-form geometries of hairpin stators and the required short inspection times, inline reconstruction and accurate quantification of relevant features is of particular importance. In this study, we propose a novel method to estimate the creepage distance, a feature that is crucial regarding the safety standards of hairpin stators and that could be determined neither automatically nor accurately until now. The data acquisition is based on fringe projection profilometry and a robot positioning system for a highly complete surface reconstruction. After alignment, the wire pairs are density-based clustered so that computations can be parallelized for each cluster, and an analysis of partial geometries is enabled. In several further steps, stripping edges are segmented automatically using a novel approach of spatially asymmetric windowed local surface normal variation, and the creepage distances are subsequently estimated using a geodesic path algorithm. Finally, the approach is examined and discussed for an entire stator, and a methodology is presented that enables the identification of implausible estimated creepage distances.
Advantages of Whole Genome Sequencing in Mitigating the Helicobacter pylori Antimicrobial Resistance Problem
Authors: Fauzia Kartika Afrida , Alfaray Ricky Indra , Yamaoka Yoshio
Published: May 2023

Helicobacter pylori antimicrobial resistance is a critical public health issue. Typically, antimicrobial resistance epidemiology reports include only the antimicrobial susceptibility test results for H. pylori. However, this phenotypic approach is less capable of answering queries related to resistance mechanisms and specific mutations found in particular global regions. Whole genome sequencing can help address these two questions while still offering quality control and is routinely validated against AST standards. A comprehensive understanding of the mechanisms of resistance should improve H. pylori eradication efforts and prevent gastric cancer.
Microbiota Regulates Pancreatic Cancer Carcinogenesis through Altered Immune Response
Authors: Chai Yihan , Huang Zhengze , Shen Xuqiu , Lin Tianyu , Zhang Yiyin , Feng Xu , Mao Qijiang , Liang Yuelong
Published: May 2023

The microbiota is present in many parts of the human body and plays essential roles. The most typical case is the occurrence and development of cancer. Pancreatic cancer (PC), one of the most aggressive and lethal types of cancer, has recently attracted the attention of researchers. Recent research has revealed that the microbiota regulates PC carcinogenesis via an altered immune response. Specifically, the microbiota, in several sites, including the oral cavity, gastrointestinal tract, and pancreatic tissue, along with the numerous small molecules and metabolites it produces, influences cancer progression and treatment by activating oncogenic signaling, enhancing oncogenic metabolic pathways, altering cancer cell proliferation, and triggering chronic inflammation that suppresses tumor immunity. Diagnostics and treatments based on or in combination with the microbiota offer novel insights to improve efficiency compared with existing therapies.
In Vitro and In Vivo Study of Combined Effect of Some Algerian Medicinal Plants and Probiotics against Helicobacter pylori
Authors: Hasna Bouhenni , Houari Hemida , Koula Doukani , Marina Spinu , Emilia Ungureanu , Assia Boumezrag
Published: May 2023

Helicobacter pylori causes gastritis, peptic ulcers and gastric cancer and affects more than half of the world’s population. Although this infection can have serious consequences, no novel cure or remedy have been discovered, the present therapy still relies on a variety of known antibiotics and anti-secretory agents. In the present study, the potential effect of combinations between methanolic extracts of four Algerian medicinal plants, garlic (Allium sativum), red onion (Allium cepa), cumin (Cuminum cyminum L.) and fenugreek (T. foenum-graecum L.), with different strains of lactic acid bacteria against H. pylori, was investigated. Similarly, the in vivo antibacterial effect of fenugreek extract combined with Bifidobacterium breve on H. pylori colonization potential to confirm the enhanced effect of the mixture was explored. Helicobacter pylori was inhibited by all combined mixtures of extracts and probiotics with varying results. The highest anti-H. pylori activities were found in fenugreek/B. breve, cumin/B. breve, garlic/B. breve and onion/B. breve combinations with inhibition diameters of 29, 26, 23 and 25 mm, respectively. Preliminary studies on the effect of probiotics against H. pylori revealed that the inhibition was due to lactic acid and bacteriocins and it was also related to the presence of phenolic compounds in the studied plants, such as gallic acid, caffeic acid, quercetin and vanillic acid. Fenugreek extract was found to inhibit the growth of H. pylori in a concentration-dependent manner. When H. pylori-infected rats were administered B. breve, the infection rate of H. pylori was significantly reduced, while the combination of B. breve and fenugreek extract effectively inhibited H. pylori. In addition, the B. breve and fenugreek extract mixture significantly reduced gastritis in H. pylori-infected rats. These results suggest that this complex mixture may be an alternative to treating diseases caused by H. pylori infection.
Human Bocavirus in Childhood: A True Respiratory Pathogen or a “Passenger” Virus? A Comprehensive Review
Authors: Trapani Sandra , Caporizzi Alice , Ricci Silvia , Indolfi Giuseppe
Published: May 2023

Recently, human bocavirus (HBoV) has appeared as an emerging pathogen, with an increasing number of cases reported worldwide. HBoV is mainly associated with upper and lower respiratory tract infections in adults and children. However, its role as a respiratory pathogen is still not fully understood. It has been reported both as a co-infectious agent (predominantly with respiratory syncytial virus, rhinovirus, parainfluenza viruses, and adenovirus), and as an isolated viral pathogen during respiratory tract infections. It has also been found in asymptomatic subjects. The authors review the available literature on the epidemiology of HBoV, the underlying risk factors associated with infection, the virus’s transmission, and its pathogenicity as a single pathogen and in co-infections, as well as the current hypothesis about the host’s immune response. An update on different HBoV detection methods is provided, including the use of quantitative single or multiplex molecular methods (screening panels) on nasopharyngeal swabs or respiratory secretions, tissue biopsies, serum tests, and metagenomic next-generations sequencing in serum and respiratory secretions. The clinical features of infection, mainly regarding the respiratory tract but also, though rarely, the gastrointestinal one, are extensively described. Furthermore, a specific focus is dedicated to severe HBoV infections requiring hospitalization, oxygen therapy, and/or intensive care in the pediatric age; rare fatal cases have also been reported. Data on tissue viral persistence, reactivation, and reinfection are evaluated. A comparison of the clinical characteristics of single infection and viral or bacterial co-infections with high or low HBoV rates is carried out to establish the real burden of HBoV disease in the pediatric population.
Robust Self-Supported SnO2-Mn2O3@CC Electrode for Efficient Electrochemical Degradation of Cationic Blue X-GRRL Dye
Authors: Li Caiyun , Yi Peng , Sun Junwei , Wang Xi-Ao , Liu Rongzhan , Sun Jiankun
Published: May 2023

Exploration of highly efficient and robust catalyst is pivotal for electrocatalytic degradation of dye wastewater, but it still is a challenge. Here, we develop a three-dimensional self-supported SnO2-Mn2O3 hybrid nanosheets grown on carbon cloth (noted by SnO2-Mn2O3@CC) electrode via a simple hydrothermal method and annealing treatment. Benefitting from the interlaced nanosheets architecture that enlarges the surface area and the synergetic component effect that accelerates the interfacial electronic transfer, SnO2-Mn2O3@CC electrode exhibits a superior electrocatalytic degradation efficiency for cationic blue X-GRRL dye in comparison with the single metal oxide electrode containing SnO2@CC and Mn2O3@CC. The degradation efficiency of cationic blue X-GRRL on SnO2-Mn2O3@CC electrode can reach up to 97.55% within 50 min. Furthermore, self-supported architecture of nanosheets on carbon cloth framework contributes to a robust stability compared with the traditional electrode via the multiple dip/brush coating accompanied by the thermal decomposition method. SnO2-Mn2O3@CC electrode exhibits excellent recyclability, which can still retain a degradation efficiency of 94.12% after six cycles. This work may provide a new pathway for the design and exploration of highly efficient and robust electrooxidation catalysts for dye degradation.
Toxicity Effects of Polystyrene Nanoplastics with Different Sizes on Freshwater Microalgae Chlorella vulgaris
Authors: Xiang Qingqing , Zhou Ying , Tan Chengxia
Published: May 2023

The ubiquitous nature of plastics, particularly nanoplastics, raises concern about their potential effects on primary producer microalgae. Currently, the impacts and potential mechanisms of nanoplastics on microalgae are not fully understood. In this study, the effects of two plain commercial polystyrene nanoplastics (PS-NPs) with different sizes (50 nm and 70 nm) on C. vulgaris were assessed in a concentration range of 0–50 mg/L during 72 h exposure periods. Results revealed that both PS-NPs have dose-dependent toxicity effects on C. vulgaris, as confirmed by the decrease of growth rates, chlorophyll a and esterase activities, and the increase of ROS, MDA, and membrane damage. The membrane damage was caused by the agglomeration of PS-NPs on microalgae and may be the key reason for the toxicity. Compared with 70 nm PS-NPs (72 h EC50 >50 mg/L), 50 nm PS-NPs posed greater adverse effects on algae, with an EC50–72h of 19.89 mg/L. FTIR results also proved the stronger variation of macromolecules in the 50 nm PS-NPs treatment group. This phenomenon might be related to the properties of PS-NPs in exposure medium. The lower absolute zeta potential value of 50 nm PS-NPs induced the stronger interaction between PS-NPs and algae as compared to 70 nm PS-NPs, leading to severe membrane damage and the loss of esterase activity as well as settlement. These findings emphasized the importance of considering the impacts of commercial PS-NPs properties in toxicity evaluation.
Anti-Atopic Effect of Isatidis Folium Water Extract in TNF-α/IFN-γ-Induced HaCaT Cells and DNCB-Induced Atopic Dermatitis Mouse Model
Authors: Min Ga-Yul , Kim Tae In , Kim Ji-Hye , Cho Won-Kyung , Yang Ju-Hye , Ma Jin Yeul
Published: May 2023

Isatidis folium or Isatis tinctoria L. is a flowering plant of the Brassicaceae family, commonly known as woad, with an ancient and well-documented history as an indigo dye and medicinal plant. This study aimed to evaluate the anti-atopic dermatitis (AD) effects of Isatidis folium water extract (WIF) using a 2,4-dinitrochlorobenzene (DNCB)-induced AD-like mouse model and to investigate the underlying mechanism using tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ)-activated HaCaT cells. Oral administration of WIF reduced spleen weight, decreased serum IgE and TNF-α levels, reduced epidermal and dermal thickness, and inhibited eosinophil and mast cell recruitment to the dermis compared to DNCB-induced control groups. Furthermore, oral WIF administration suppressed extracellular signal-regulated kinase and p38 mitogen-activated protein kinase protein expression levels, p65 translocation from the cytoplasm to the nucleus, and mRNA expression of TNF-α, IFN-γ, interleukin (IL)-6, and IL-13 in skin lesion tissues. In HaCaT cells, WIF suppressed the production of regulated upon activation, normal T cell expressed and secreted (RANTES), thymus and activation-regulated chemokine (TARC), macrophage-derived chemokine (MDC), MCP-1, and MIP-3a, which are inflammatory cytokines and chemokines related to AD, and inhibited the mRNA expression of RANTES, TARC, and MDC in TNF-α/IFN-γ-stimulated HaCaT cells. Overall, the results revealed that WIF ameliorated AD-like skin inflammation by suppressing proinflammatory cytokine and chemokine production via nuclear factor-κB pathway inhibition, suggesting WIF as a potential candidate for AD treatment.
Mitochondria-Targeted Fluorescent Nanoparticles with Large Stokes Shift for Long-Term BioImaging
Authors: Li Xiao , Zhang Tao , Diao Xuebo , Yu Li , Su Yue , Yang Jiapei , Shang Zibo , Liu Shuai , Zhou Jia , Li Guolin , Chi Huirong
Published: May 2023

Mitochondria (MITO) play a significant role in various physiological processes and are a key organelle associated with different human diseases including cancer, diabetes mellitus, atherosclerosis, Alzheimer’s disease, etc. Thus, detecting the activity of MITO in real time is becoming more and more important. Herein, a novel class of amphiphilic aggregation-induced emission (AIE) active probe fluorescence (AC-QC nanoparticles) based on a quinoxalinone scaffold was developed for imaging MITO. AC-QC nanoparticles possess an excellent ability to monitor MITO in real-time. This probe demonstrated the following advantages: (1) lower cytotoxicity; (2) superior photostability; and (3) good performance in long-term imaging in vitro. Each result of these indicates that self-assembled AC-QC nanoparticles can be used as effective and promising MITO-targeted fluorescent probes.
Extraction of Isoflavones, Alpha-Hydroxy Acids, and Allantoin from Soybean Leaves—Optimization by a Mixture Design of the Experimental Method
Authors: Dresler Sławomir , Strzemski Maciej , Baczewska Izabela , Koselski Mateusz , Hassanpouraghdam Mohammad , Szczepanek Dariusz , Sowa Ireneusz , Wójciak Magdalena , Hanaka Agnieszka
Published: May 2023

Soybeans are commonly known as a valuable source of biologically active compounds including isoflavones as well as allantoin and alpha-hydroxy acids. Since these compounds exhibit skin therapeutic effects, they are widely used in the cosmetic and pharmaceutical industries. The presented paper shows the optimization of three solvent systems (ethanol, water, and 1,3-propanediol) to increase the extraction efficiency of isoflavones (daidzin, genistin, 6″-O-malonyldaidzin, 6″-O-malonylglycitin, 6″-O-malonylgenistin), allantoin, and alpha-hydroxy acids (citric acid, malic acid) from soybean leaves. A simplex centroid mixture design for three solvents with interior points was applied for the experimental plan creation. Based on the obtained results of metabolite extraction yield in relation to solvent composition, polynomial regression models were developed. All models were significant, with predicted R-squared values between 0.77 and 0.99, while in all cases the model’s lack of fit was not significant. The optimal mixture composition enabling the maximization of extraction efficiency was as follows: 32.9% ethanol, 53.9% water, and 13.3% propanediol (v/v/v). Such a mixture composition provided the extraction of 99%, 91%, 100%, 92%, 99%, 70%, 92%, and 69% of daidzin, genistin, 6″-O-malonyldaidzin, 6″-O-malonylglycitin, 6″-O-malonylgenistin, allantoin, citric acid, and malic acid, respectively. The solvent mixture composition developed provides a good extraction efficiency of the metabolites from soybean leaves and high antioxidant properties.
Mechanistic Studies of Arene–Ruthenium(II) Complexes with Carbothioamidopyrazoles as Alternative Cancer Drugs
Authors: Hikisz Paweł , Namiecińska Ewelina , Paneth Piotr , Budzisz Elzbieta
Published: May 2023

Arene–ruthenium(II) complexes with carbothioamidopyrazoles at the C-2 and C-5 positions have been recognized as chemotherapeutic agent alternatives to cisplatin and its oxaliplatin analogs. The aim of this study was to continue research on the biological aspect of arene–ruthenium(II) complexes and their anticancer activity. The present paper includes an additional 12 new tumor cells, analyzed by MTT, and employs a series of extended bioassays to better understand their potential mechanism of antitumor activity. The following tests were conducted: membrane permeability studies, intramolecular reactive oxygen and nitrogen species (ROS/RNS) assays, mitochondrial potential changes, DNA analysis by comet assay using the electrophoresis method, measurement of cleaved PARP protein levels, and determination of apoptotic and necrotic cell fractions by fluorescence microscopy. Additionally, the article presents lipophilicity studies based on RP-TLC and molecular docking studies. We hope that the presented data will prove useful in practical treatment, especially for patients with cancer.
Non-Native Structures of Apomyoglobin and Apoleghemoglobin in Folding Intermediates Related to the Protein Misfolding
Authors: Nishimura Chiaki , Kikuchi Takeshi
Published: May 2023

Protein folding is essential for a polypeptide chain to acquire its proper structure and function. Globins are a superfamily of ubiquitous heme-binding α-helical proteins whose function is principally to regulate oxygen homoeostasis. In this review, we explore the hierarchical helical formation in the globin proteins apomyoglobin and leghemoglobin, and we discuss the existence of non-native and misfolded structures occurring during the course of folding to its native state. This review summarizes the research aimed at characterizing and comparing the equilibrium and kinetic intermediates, as well as delineating the complete folding pathway at a molecular level, in order to answer the following questions: “What is the mechanism of misfolding via a folding intermediate? Does the non-native structure stabilize the contemporary intermediate structure? Does the non-native structure induce slower folding?” The role of the non-native structures in the folding intermediate related to misfolding is also discussed.
Comparison between Cashew-Based and Petrochemical Hydroxyoximes: Insights from Molecular Simulations
Authors: Nguyen Cuong V. , Phan Chi M. , Hoang Son A. , Yusa Shin-ichi
Published: May 2023

Solvent extraction has been ubiquitously used to recover valuable metals from wastes such as spent batteries and electrical boards. With increasing demands for energy transition, there is a critical need to improve the recycling rate of critical metals, including copper. Therefore, the sustainability of reagents is critical for the overall sustainability of the process. Yet, the recycling process relies on functional organic compounds based on the hydroxyoxime group. To date, hydroxyoxime extractants have been produced from petrol-based chemical feedstocks. Recently, natural-based cardanol has been used to produce an alternative hydroxyoxime. The natural-based oxime has been employed to recover valuable metals (Ga, Ni, Co) via a liquid/liquid extraction process. The natural compound has a distinctive structure with 15 carbons in the alkyl tail. In contrast, petrol-based hydroxyoximes have only 12 or fewer carbons. However, the molecular advantages of this natural-based compound over the current petrol-based ones remain unclear. In this study, molecular dynamics simulation was employed to investigate the effect of extractant hydrocarbon chains on the extraction of copper ions. Two hydroxyoxime extractants with 12 and 15 carbons in the alkyl chain were found to have similar interactions with Cu2+ ions. Yet, a slight molecular binding increase was observed when the carbon chain was increased. In addition, lengthening the carbon chain made the extracting stage easier and the stripping stage harder. The binding would result in a lower pH in the extraction step and a lower pH in the stripping step. The insights from this molecular study would help design the extraction circuit using natural-based hydroxyoxime extractants. A successful application of cashew-based cardanol will improve the environmental benefits of the recycling process. With cashew-producing regions in developing countries, the application also improves these regions’ social and economic sustainability.
High-Contrast Visualization Chemiluminescence Based on AIE-Active and Base-Sensitive Emitters
Authors: Zhang Xiao-Wen , Chen Xu-Lin , Lu Can-Zhong
Published: May 2023

Peroxyoxalate chemiluminescence (PO-CL) is one of the most popular cold light sources, yet the drawback of aggregation-caused quenching limits their use. Here, we report a new kind of efficient bifunctional emitter derived from salicylic acid, which not only exhibits typical aggregation-induced emission (AIE) character but also has the ability to catalyze the CL process under basic conditions based on base sensitivity. By taking advantage of these unique features, we successfully confine the CL process on the surface of solid bases and provide a high-contrast visualization of CL emission. This method allows most of the common basic salts like sodium carbonate to be invisible encryption information ink and PO-CL solution to be a decryption tool to visualize the hidden information. The current study opens up an appealing way for the development of multifunction CL emitters for information encryption and decryption applications.
Palladium-Catalyzed Direct (Het)arylation Reactions of Benzo[1,2-d:4,5-d′]bis([1,2,3]thiadiazole and 4,8-Dibromobenzo[1,2-d:4,5-d′]bis([1,2,3]thiadiazole)
Authors: Chmovzh Timofey N. , Kudryashev Timofey A. , Alekhina Daria A. , Rakitin Oleg A.
Published: May 2023

Palladium-catalyzed direct (het)arylation reactions of strongly electron-withdrawing tricyclic benzo[1,2-d:4,5-d′]bis([1,2,3]thiadiazole) and its 4,8-dibromo derivative were studied; the conditions for the selective formation of mono- and bis-aryl derivatives were found. The reaction of 4,8-dibromobenzo[1,2-d:4,5-d′]bis([1,2,3]thiadiazole) with thiophenes in the presence of palladium acetate as a catalyst and potassium pivalate as a base, depending on the conditions used, selectively gave both mono- and bis-thienylated benzo-bis-thiadiazoles in low to moderate yields; arenes were found to be inactive in these reactions. It was discovered that direct C–H arylation of benzo[1,2-d:4,5-d′]bis([1,2,3]thiadiazole with bromo(iodo)arenes and -thiophenes in the presence of Pd(OAc)2 and di-tert-butyl(methyl)phosphonium tetrafluoroborate salt is a powerful tool for the selective formation of 4-mono- and 4,8-di(het)arylated benzo-bis-thiadiazoles. Oxidative double C–H hetarylation of benzo[1,2-d:4,5-d′]bis([1,2,3]thiadiazole with thiophenes in the presence of Pd(OAc)2 and silver (I) oxide in DMSO was successfully employed to prepare bis-thienylbenzo-bis-thiadiazoles in moderate yields.