bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental Health Awareness: Stigma and Help-Seeking Among Portuguese College Students
Authors: Carvalho Paula Saraiva , Pombal Nádia , Gama Jorge , Loureiro Manuel
Published: December 2024

Background/Objectives: Help-seeking—that is, the attempt to attain external help for mental health, be it from formal or informal sources—can be described as an adaptive coping process. Mental illness stigma is the most frequently identified barrier that prevents students from seeking psychological help. This study analyzed college students’ beliefs about mental illness and attitudes toward formal psychological help-seeking. Methods: Two hundred and eighty-two students from the first and third undergraduate years of Psychology, Sociology, Fashion Design, and Sports Science courses participated. The majority of the sample (75.4%) was female, while only 24.6% was male, with a mean age of 20.04 years. The scales used were the Inventory of Beliefs about Mental Illness (IBMI), the Inventory of Attitudes Toward Seeking Mental Health Services (IATSMHS), and a sociodemographic questionnaire. Results: The results revealed that females, third-year students, and Psychology students had fewer stigmatizing beliefs about mental illness and more positive attitudes towards help-seeking. Moreover, there were statistically significant differences in beliefs and attitudes in relation to psychological support and familiarity with mental illness. We also glimpsed the impact of the COVID-19 pandemic on students’ mental health, and observed a change in opinions and attitudes toward mental illness during this period. Correlation analysis showed negative correlations between stigmatizing beliefs and attitudes toward seeking help. Finally, a cluster analysis identified two profiles of individuals that reflected different levels of stigma and help-seeking attitudes. Conclusions: This study delineated two distinct groups of students, which is relevant as it allows us to trace profiles to outline more uniform intervention groups and, in turn, implement new and improved interventions that are better adapted to the specific needs of college students.
Identifying the Black Country’s Top Mental Health Research Priorities Using a Collaborative Workshop Approach: Community Connexions
Authors: Morrissey Hana , Benoit Celine , Ball Patrick Anthony , Ackom-Mensah Hannah
Published: December 2024

Background: The Black Country (BC) is an area of the United Kingdom covering Dudley, Sandwell, Walsall, and Wolverhampton. The area is ethnically, culturally and religiously diverse. One-fifth of the total population is in the lowest socioeconomic quintile, with an uneven distribution of wealth. The area manifests unmet needs and as perceived underserved community groups. Objectives and Methods: To better understand the situation and inform future provision, listening events were organised across the BC to engage with local underserved communities. A mixed-methods design was employed, using collaborative workshops. The workshops enabled stakeholders to explore priorities, perceived barriers and solutions to mental health services’ access within the BC. Results: Sixty participants verbally consented and signed in to attend the three workshops. There were nine groups that provided 247 statements on the topic, yielding a total of 12 codes and six themes (priorities). The top identified priorities were inappropriate periodisation of accessible funded healthcare needs (n = 42, 18.03%), barriers to appropriate healthcare (n = 49, 21.03%) and limited resources for training, health promotion, preventative care and support networks (n = 62, 26.61%). Conclusions: Addressing the identified priorities will require location and community-specific solutions to establish those communities’ trust and engagement. Cultural stigma should not be viewed as the only barrier to access healthcare but should be considered in combination with the population’s reluctance to reach out to healthcare services due to loss of trust between community groups and lack of co-design of culturally and religiously appropriate services for the community.
Transcultural Adaptation of the Interpersonal Communication Competence Scale (ICCS) in Spanish Health Sciences Undergraduate Students
Authors: Sancho-Cantus David , Álvarez-Nölting Pablo , Privado Jesús , Cubero-Plazas Laura , Botella-Navas Marta , Cañabate-Ros Montserrat
Published: December 2024

Background: The communication skills of healthcare professionals directly impact patient interventions. Consequently, an improvement in healthcare quality indicators is observed. Objective: to adapt and validate the Interpersonal Communication Competence Scale (ICCS) in a Spanish university sample of health science students. Methods: A cross-sectional design was employed with a sample of 803 undergraduate students, utilizing the ICCS. Results: The structure and internal consistency of both the 30-item test and a short form version were analyzed. Additionally, factorial invariance, differential validity by gender, and the convergent and discriminant validity of the instrument were assessed. Benchmarks were also calculated. The complete scale, after removing five items, exhibited a total internal consistency of 0.721 and demonstrated a good fit to a hierarchical model with nine first-order factors and one second-order factor (GFI = 0.921, SRMR = 0.071). The 10-item short scale exhibited an internal consistency of 0.689 and fit a single-factor model (GFI = 0.977, SRMR = 0.059). Additionally, factorial invariance was established based on gender. Concerning convergent validity, the ICCS scales correlated with similar measures from the Social Skills Questionnaire and the Health Professional’s Communication Skills Scale. Regarding differential validity, significant differences were found only in self-disclosure, empathy, and immediacy, with higher scores in all three cases for women. Conclusions: The Spanish version of the ICCS demonstrates good psychometric properties and is a valid tool for assessing interpersonal communication competencies, both generally and specifically within health sciences. Additionally, the establishment of benchmarks in the ICCS will enable future evaluators to identify the position of their assessors relative to a university sample.
Can Informal Care Be a Substitute for Formal Care? Evidence from Older People with Disabilities in Beijing, China
Authors: Cai Jiaoli , Jiang Nan , Coyte Peter C.
Published: December 2024

Background: The demand for long-term care is increasing as the elderly population continues to grow, prompting a critical examination of care modalities. Methods: This study employs data from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) to assess the relationship between informal and formal care for older people with disabilities in Beijing. The analysis employs the Probit model and incorporates the application of instrumental variable techniques and propensity score matching to ensure robustness in the results. Results: The findings highlight the important role of informal care. Conclusions: Policymakers should incorporate support policies for informal care into the overall design of the system, provide support to informal caregivers, and reduce their burden. Our research conclusions provide empirical reference for cities with similar characteristics to Beijing.
LCLN-CA: A Survival Regression Analysis-Based Prediction Method for Catechin Content in Yunnan Sun-Dried Tea
Authors: Li Hongxu , Wang Qiaomei , Wang Houqiao , Li Limei , Wang Xinghua , Wu Tianyu , Wang Chun , Qian Ye , Wang Xiaohua , Xia Yuxin , Xie Jin , Yuan Wenxia , Wang Baijuan
Published: December 2024

Catechins are pivotal determinants of tea quality, with soil environmental factors playing a crucial role in the synthesis and accumulation of these compounds. To investigate the impact of changes in tea garden soil environments on the catechin content in sun-dried tea, this study measured the catechin content in soil samples and corresponding tea leaves from Nanhua, Yunnan, China. By integrating the variations in catechin content with those of 17 soil factors and employing COX regression factor analysis, it was found that pH, organic matter (OM), fluoride, arsenic (As), and chromium (Cr) were significantly correlated with catechin content (p < 0.05). Further, using the LASSO regression for variable selection, a model named LCLN-CA was constructed with four variables including pH, OM, fluoride, and As. The LCLN-CA model demonstrated high fitting accuracy with AUC values of 0.674, 0.784, and 0.749 for catechin content intervals of CA ≤ 10%, 10% < CA ≤ 20%, and 20% < CA ≤ 30% in the training set, respectively. The validation set showed AUC values of 0.630, 0.756, and 0.723, respectively, indicating a well-calibrated curve. Based on the LCLN-CA model and the DynNom framework, a visual prediction system for catechin content in Yunnan sun-dried tea was developed. External validation with a test dataset achieved an Accuracy of 0.870. This study explored the relationship between soil-related factors and variations in catechin content, paving a new way for the prediction of catechin content in tea and enhancing the practical application value of artificial intelligence technology in agricultural production.
Fine-Mapping of Tomato male sterile-24 Locus and Marker Development for ms-24 and Its Alleles, ms-10, ms-35, and ms-36
Authors: Zhang Li , Yang Mengxia , Wei Kai , Yang Wencai , Li Shanshan , Wang Xiaoxuan , Guo Yanmei , Liu Lei , Zhu Can , Du Yongchen , Li Junming , Li Xin , Huang Zejun
Published: December 2024

Male sterility is an essential trait for minimizing costs and ensuring seed purity in the production of tomato hybrid seeds. However, its application in commercial breeding faces challenges such as instability, low stigma exsertion rates, and the lack of molecular markers to facilitate the efficient backcrossing of male sterile loci into elite tomato lines. This study characterized the male sterile-24 (ms-24) mutant, which demonstrated exserted stigmas and complete male sterility. Our histological examination revealed that ms-24 anthers displayed dysfunctional development of the tetrads and tapetum. The ms-24 locus was finely mapped to a 149 kb interval containing 22 putative genes. Among these, Solyc02g079810, also known as MS-10, encodes a basic helix–loop–helix (bHLH) transcription factor essential for tapetum and pollen development. A sequencing analysis revealed an approximately 4.9 kb retrotransposon insertion in the first exon of the MS-10 gene in ms-24. Allelism tests confirmed that ms-24 was allelic to ms-10, which is also allelic to ms-35 and ms-36. The same retrotransposon insertion was also identified in the ms-10 mutant, and a similar insertion of retrotransposon was detected in the second exon of the MS-10 gene in the ms-36 mutant. Based on these retrotransposon sequences, codominant insertion–deletion (InDel) markers, MS-24I and MS-35I, were developed for the precise identification of the ms-24, ms-10, ms-35, and ms-36 alleles. These findings establish a foundational basis for subsequent investigations into the molecular mechanisms underlying male sterility and enhance the selection process of male sterile lines in tomato hybrid breeding programs.
The Relationship Between Body, Mind, and Social Characteristics in a Sample of a Training Program for Developing Social and Personal Resources: A Network Analysis
Authors: Janka Christoph , Höcker Maike , Dorner Thomas E.
Published: December 2024

In this cross-sectional study, the interactions between demographic characteristics and the body, mind, and social dimensions among participants of a holistic training program for social and personal development were examined and compared to a control group. The sample involved 223 adults from Austria, Germany, and Switzerland, with 80 (37.2%) participants in the training group already having finished the training. To explore the variable relationships and compare group structures, advanced network analyses and a network comparison test were applied. The network analysis identified the training as a central variable, being linked to aspirations for societal impact and personal growth (0.31), aspirations for the pursuit of fame and wealth (0.29), transcendence (0.19), the desire to improve the quality of relationships (0.19), aspirations for personal wellbeing and relationships (0.15), and the presence of social resources (0.13). The group comparison revealed that the control group primarily connected through the “Sense of Coherence—Meaning in Life” axis, whereas the training group showed more complex linkages involving wellbeing, life satisfaction, meaning in life, and transcendence, underlining the training program’s positive effect on personal growth, societal impact aspirations, and transcendence. Overall, the network comparison test revealed significant differences in network structure and overall connectivity between the training and the control group.
Workers’ Injury Risks Focusing on Body Parts in Reinforced Concrete Construction Projects
Authors: Lim Jiseon , Cho Jaehong , Kim Jeonghwan , Kang Sanghyeok
Published: December 2024

This study addresses occupational safety in reinforced concrete construction, an area marked by high accident rates and significant worker injury risks. By focusing on activity–body part (A–BP) combinations, this research introduces a novel framework for quantifying injury risks across construction activities. Reinforced concrete construction tasks are categorized into ten specific activities within three major work types: rebar work, formwork, and concrete placement. These are further analyzed concerning six critical body parts frequently injured on-site: head/face, arm/shoulder, wrist/hand, torso, leg/pelvis, and foot/ankle. Using data from 2283 construction accident reports and expert surveys, the probability and severity of injuries for each A–BP element were calculated. Probability scores were derived from actual incident data, while severity scores were determined via expert evaluations, considering injury impact and the required recovery time. To ensure precision and comparability, scores were standardized across scales, enabling a final risk assessment for each A–BP. Results identified that wrist and hand injuries during rebar work activities, particularly cutting and shaping, exhibited the highest risk, underscoring the need for focused protective measures. This study contributes to construction safety management by providing detailed insights into injury risk based on activity–body part interactions, offering safety managers data-driven recommendations for tailored protective equipment, enhanced training, and preventive protocols. This research framework not only helps optimize safety interventions on conventional construction sites but also establishes a basis for future studies aimed at adapting these strategies to evolving construction methods.
Continuous Satellite Image Generation from Standard Layer Maps Using Conditional Generative Adversarial Networks
Authors: Šidlauskas Arminas , Kriščiūnas Andrius , Čalnerytė Dalia
Published: December 2024

Satellite image generation has a wide range of applications. For example, parts of images must be restored in areas obscured by clouds or cloud shadows or areas that must be anonymized. The need to cover a large area with the generated images faces the challenge that separately generated images must maintain the structural and color continuity between the adjacent generated images as well as the actual ones. This study presents a modified architecture of the generative adversarial network (GAN) pix2pix that ensures the integrity of the generated remote sensing images. The pix2pix model comprises a U-Net generator and a PatchGAN discriminator. The generator was modified by expanding the input set with images representing the known parts of ground truth and the respective mask. Data used for the generative model consist of Sentinel-2 (S2) RGB satellite imagery as the target data and OpenStreetMap mapping data as the input. Since forested areas and fields dominate in images, a Kneedle clusterization method was applied to create datasets that better represent the other classes, such as buildings and roads. The original and updated models were trained on different datasets and their results were evaluated using gradient magnitude (GM), Fréchet inception distance (FID), structural similarity index measure (SSIM), and multiscale structural similarity index measure (MS-SSIM) metrics. The models with the updated architecture show improvement in gradient magnitude, SSIM, and MS-SSIM values for all datasets. The average GMs of the junction region and the full image are similar (do not exceed 7%) for the images generated using the modified architecture whereas it is more than 13% higher in the junction area for the images generated using the original architecture. The importance of class balancing is demonstrated by the fact that, for both architectures, models trained on the dataset with a higher ratio of classes representing buildings and roads compared to the models trained on the dataset without clusterization have more than 10% lower FID (162.673 to 190.036 for pix2pix and 173.408 to 195.621 for the modified architecture) and more than 5% higher SSIM (0.3532 to 0.3284 for pix2pix and 0.3575 to 0.3345 for the modified architecture) and MS-SSIM (0.3532 to 0.3284 for pix2pix and 0.3575 to 0.3345 for the modified architecture) values.
It Is Not Just About Storing Energy: The Multifaceted Role of Creatine Metabolism on Cancer Biology and Immunology
Authors: Geng Yuheng , DeLay Susan L. , Chen Xiaoyang , Miska Jason
Published: December 2024

Creatine, a naturally occurring compound in mammals, is crucial in energy metabolism, particularly within muscle and brain tissues. While creatine metabolism in cancer has been studied for several decades, emerging studies are beginning to clarify the sometimes-contradictory role creatine has in either the promotion or inhibition of cancer. On one hand, creatine can directly enhance anti-tumor CD8+ T-cell activity and induce tumor apoptosis, contributing to antitumor immunity. Conversely, other studies have shown that creatine can facilitate cancer cell growth and migration by providing an energy source and activating several signaling pathways. This review will examine what is known about creatine in cancer biology, with a focus on understanding its roles across different cellular compartments. Lastly, we discuss the emerging roles of creatine metabolism, providing exciting new insights into this often-overlooked pathway. This review highlights the complex role of creatine in cancer development and treatment, offering insights into its potential as both a therapeutic target and a risk factor in oncogenesis.
IL-6 Does Not Influence the Expression of SLC41A1 and Other Mg-Homeostatic Factors
Authors: Brodnanova Maria , Cibulka Michal , Grendar Marian , Gondas Eduard , Kolisek Martin
Published: December 2024

Together with chronic inflammation, disturbed magnesium homeostasis is a factor accompanying chronic disease which thus contributes to a reduced quality of human life. In this study, our objective was to examine the possible IL-6-mediated chronic inflammation-dependent regulation of nine magnesiotropic genes encoding for constituents of magnesium homeostasis of the cell. We used three cell lines (HepG2, U-266, and PANC-1), all characterized by high expression of the IL6R gene and the presence of a membrane form of IL-6R capable of responding to human IL-6. Despite the confirmed activation of the IL-6R/JAK/STAT3 pathway after hIL-6 treatment, we observed no biologically relevant changes in the transcription intensity of the studied magnesiotropic genes. This, however, does not exclude the possibility that IL-6 can affect magnesium homeostasis at levels other than through modified transcription.
Bridging the Gap: Endothelial Dysfunction and the Role of iPSC-Derived Endothelial Cells in Disease Modeling
Authors: Sgromo Chiara , Cucci Alessia , Venturin Giorgia , Follenzi Antonia , Olgasi Cristina
Published: December 2024

Endothelial cells (ECs) are crucial for vascular health, regulating blood flow, nutrient exchange, and modulating immune responses and inflammation. The impairment of these processes causes the endothelial dysfunction (ED) characterized by oxidative stress, inflammation, vascular permeability, and extracellular matrix remodeling. While primary ECs have been widely used to study ED in vitro, their limitations—such as short lifespan and donor variability—pose challenges. In this context, induced iECs derived from induced pluripotent stem cells offer an innovative solution, providing an unlimited source of ECs to explore disease-specific features of ED. Recent advancements in 3D models and microfluidic systems have enhanced the physiological relevance of iEC-based models by better mimicking the vascular microenvironment. These innovations bridge the gap between understanding ED mechanisms and drug developing and screening to prevent or treat ED. This review highlights the current state of iEC technology as a model to study ED in vascular and non-vascular disorders, including diabetes, cardiovascular, and neurodegenerative diseases.
Lithium, Inflammation and Neuroinflammation with Emphasis on Bipolar Disorder—A Narrative Review
Authors: Damri Odeya , Agam Galila
Published: December 2024

This narrative review examines lithium’s effects on immune function, inflammation and cell survival, particularly in bipolar disorder (BD) in in vitro studies, animal models and clinical studies. In vitro studies show that high lithium concentrations (5 mM, beyond the therapeutic window) reduce interleukin (IL)-1β production in monocytes and enhance T-lymphocyte resistance, suggesting a protective role against cell death. Lithium modulates oxidative stress in lipopolysaccharide (LPS)-activated macrophages by inhibiting nuclear factor (NF)-ƙB activity and reducing nitric oxide production. At therapeutically relevant levels, lithium increased both pro-inflammatory [interferon (INF)-γ, IL-8 and tumor necrosis factor (TNF)-α)] and anti-inflammatory (IL-10) cytokines on whole blood supernatant culture in healthy volunteers, influencing the balance of pro- and anti-inflammatory responses. Animal models reveal lithium’s potential to alleviate inflammatory diseases by reducing pro-inflammatory cytokines and enhancing anti-inflammatory responses. It also induces selective macrophage death in atherosclerotic plaques without harming other cells. In primary rat cerebellum cultures (ex vivo), lithium prevents neuronal loss and inhibits astroglial growth, impacting astrocytes and microglia. Clinical studies show that lithium alters cytokine profiles and reduces neuroinflammatory markers in BD patients. Chronic treatment decreases IL-2, IL-6, IL-10 and IFN-γ secretion from peripheral blood leukocytes. Lithium response correlates with TNF-α levels, with poor responders showing higher TNF-α. Overall, these findings elucidate lithium’s diverse mechanisms in modulating immune responses, reducing inflammation and promoting cell survival, with significant implications for managing BD and other inflammation-related conditions. Yet, to better understand the drug’s impact in BD and other inflammatory/neuroinflammatory conditions, further research is warranted to appreciate lithium’s therapeutic potential and its role in immune regulation.
Evolution and Function of MADS-Box Transcription Factors in Plants
Authors: Zhang Zihao , Zou Wenhui , Lin Peixia , Wang Zixun , Chen Ye , Yang Xiaodong , Zhao Wanying , Zhang Yuanyuan , Wang Dongjiao , Que Youxiong , Wu Qibin
Published: December 2024

The MADS-box transcription factor (TF) gene family is pivotal in various aspects of plant biology, particularly in growth, development, and environmental adaptation. It comprises Type I and Type II categories, with the MIKC-type subgroups playing a crucial role in regulating genes essential for both the vegetative and reproductive stages of plant life. Notably, MADS-box proteins can influence processes such as flowering, fruit ripening, and stress tolerance. Here, we provide a comprehensive overview of the structural features, evolutionary lineage, multifaceted functions, and the role of MADS-box TFs in responding to biotic and abiotic stresses. We particularly emphasize their implications for crop enhancement, especially in light of recent advances in understanding the impact on sugarcane (Saccharum spp.), a vital tropical crop. By consolidating cutting-edge findings, we highlight potential avenues for expanding our knowledge base and enhancing the genetic traits of sugarcane through functional genomics and advanced breeding techniques. This review underscores the significance of MADS-box TFs in achieving improved yields and stress resilience in agricultural contexts, positioning them as promising targets for future research in crop science.
Protein and Polysaccharide Fibers via Air Jet Spinning: Emerging Techniques for Biomedical and Sustainable Applications
Authors: Prahaladan Varsha , Poluri Nagireddy , Napoli Makara , Castro Connor , Yildiz Kerem , Berry-White Brea-Anna , Lu Ping , Salas-de la Cruz David , Hu Xiao
Published: December 2024

Polymers play a critical role in the biomedical and sustainable materials fields, serving as key resources for both research and product development. While synthetic and natural polymers are both widely used, synthetic polymers have traditionally dominated due to their ability to meet the specific material requirements of most fiber fabrication methods. However, synthetic polymers are derived from non-renewable resources, and their production raises environmental and health concerns. Natural polymers, on the other hand, are derived from renewable biological sources and include a subset known as biopolymers, such as proteins and polysaccharides, which are produced by living organisms. These biopolymers are naturally abundant and offer benefits such as biodegradability and non-toxicity, making them especially suitable for biomedical and green applications. Recently, air jet spinning has emerged as a promising method for fabricating biopolymer fibers, valued for its simplicity, cost-effectiveness, and safety—advantages that stand out compared to the more conventional electrospinning process. This review examines the methods and mechanisms of air jet spinning, drawing on empirical studies and practical insights to highlight its advantages over traditional fiber production techniques. By assembling natural biopolymers into micro- and nanofibers, this novel fabrication method demonstrates strong potential for targeted applications, including tissue engineering, drug delivery, air filtration, food packaging, and biosensing, utilizing various protein and polysaccharide sources.
Damage of the Bone Marrow Stromal Precursors in Patients with Acute Leukemia at the Onset of the Disease and During Treatment
Authors: Sadovskaya Aleksandra , Petinati Nataliya , Shipounova Irina , Drize Nina , Smirnov Igor , Pobeguts Olga , Arapidi Georgiy , Lagarkova Maria , Karaseva Luiza , Pokrovskaya Olga , Kuzmina Larisa , Vasilieva Anastasia , Aleshina Olga , Parovichnikova Elena
Published: December 2024

In patients with acute leukemia (AL), malignant cells and therapy modify the properties of multipotent mesenchymal stromal cells (MSCs) and their descendants, reducing their ability to maintain normal hematopoiesis. The aim of this work was to elucidate the alterations in MSCs at the onset and after therapy in patients with AL. The study included MSCs obtained from the bone marrow of 78 AL patients (42 AML and 36 ALL) and healthy donors. MSC growth characteristics, gene expression pattern, proteome and secretome were studied using appropriate methods. The concentration of MSCs in the bone marrow, proliferative potential, the expression of several genes, proteomes and secretomes were altered in AL-MSCs. Stromal progenitors had been affected differently in ALL and AML patients. In remission, MSC functions remain impaired despite the absence of tumor cells and the maintenance of benign hematopoietic cells. AL causes crucial and, to a large extent, irreversible changes in bone marrow MSCs.
Artificial Tertiary Lymphoid Structures: Exploring Mesenchymal Stromal Cells as a Platform for Immune Niche Formation
Authors: Zubkova Ekaterina , Kalinin Alexander , Beloglazova Irina , Kurilina Ella , Menshikov Mikhail , Parfyonova Yelena , Tsokolaeva Zoya
Published: December 2024

Constructing artificial tertiary lymphoid structures (TLSs) opens new avenues for advancing cancer immunotherapy and personalized medicine by creating controllable immune niches. Mesenchymal stromal cells (MSCs) offer an ideal stromal source for such constructs, given their potent immunomodulatory abilities and accessibility. In this study, we explored the potential of adipose-derived MSCs to adopt TLS-supportive phenotypes and facilitate lymphocyte organization. Single-cell RNA sequencing revealed a distinct subpopulation of MSCs expressing key fibroblastic reticular cell (FRC)-associated markers, including IL-7, PDPN, and IL-15, though lacking follicular dendritic cell (FDC) markers. TNF-α stimulation, but not LTα2β1, further enhanced FRC marker expression (IL-7, PDPN, and ICAM1). Notably, in 3D spheroid co-culture with lymphocytes, MSCs upregulated additional FRC markers, specifically CCL21. Upon implantation into adipose tissue, MSC-lymphocyte organoids maintained structural integrity and showed extensive T-cell infiltration and partial vascularization after 15 days in vivo, although organized B-cell follicles and FDC markers were still lacking. These findings highlight MSCs’ intrinsic ability to adopt an FRC-like phenotype that supports T-cell and HEV organization, suggesting that further optimization, including genetic modification, may be needed to achieve an FDC phenotype and replicate the full architectural and functional complexity of TLSs.
Cannabis sativa: From Plants to Humans
Authors: Trono Daniela
Published: December 2024

This Special Issue aims to highlight some of the most recent developments in the study of Cannabis sativa by collecting contributions that range from studies on the crop and its interaction with the environment and pathogens to the pharmaceutical applications of cannabinoid-based drugs, also including the health risks associated with the consumption of C [...]
Compared Inhibitory Activities of Tamoxifen and Avenanthramide B on Liver Esterase and Correlation Based on the Superimposed Structure Between Porcine and Human Liver Esterase
Authors: Lim Hakseong , Hwang Sungbo , Cho Seung-Hak , Bak Young-Seok , Yang Woong-Suk , Park Daeui , Kim Cheorl-Ho
Published: December 2024

Exposure to tamoxifen can exert effects on the human liver, and esterases process prodrugs such as antibiotics and convert them to less toxic metabolites. In this study, the porcine liver esterase (PLE)-inhibitory activity of tamoxifen has been investigated. PLE showed inhibition of a PLE isoenzyme (PLE5). In addition, avenanthramides, which have a similar structure to that of tamoxifen, have been used to determine the PLE-inhibitory effect. Among the avenanthramide derivatives, avenanthramide B has been shown to inhibit PLE. Avenanthramide B interacts with Lys284 of PLE, whereas avenanthramide A and C counteract with Lys284. Avenanthramide B has shown a similar inhibitory effect to that of tamoxifen. Given that avenanthramide B can modulate the action of PLE, it can be used in pharmaceutical and industrial applications for modulating the effects of PLE. Based on superimposed structures between PLE and human liver esterase, the impact of tamoxifen use in humans is discussed. In addition, this study can serve as a fundamental basis for future investigations regarding the potential risk of tamoxifen and other drugs. Thus, this study presents an insight into the comparison of structurally similar tamoxifen and avenanthramides on liver esterases, which can have implications for the pharmaceutical and agricultural industries.
Systemic and Cardiac Microvascular Dysfunction in Hypertension
Authors: Durante Alessandro , Mazzapicchi Alessandro , Baiardo Redaelli Martina
Published: December 2024

Hypertension exerts a profound impact on the microcirculation, causing both structural and functional alterations that contribute to systemic and organ-specific vascular damage. The microcirculation, comprising arterioles, capillaries, and venules with diameters smaller than 20 μm, plays a fundamental role in oxygen delivery, nutrient exchange, and maintaining tissue homeostasis. In the context of hypertension, microvascular remodeling and rarefaction result in reduced vessel density and elasticity, increasing vascular resistance and driving end-organ damage. The pathophysiological mechanisms underlying hypertensive microvascular dysfunction include endothelial dysfunction, oxidative stress, and excessive collagen deposition. These changes impair nitric oxide (NO) bioavailability, increase reactive oxygen species (ROS) production, and promote inflammation and fibrosis. These processes lead to progressive vascular stiffening and dysfunction, with significant implications for multiple organs, including the heart, kidneys, brain, and retina. This review underscores the pivotal role of microvascular dysfunction in hypertension-related complications and highlights the importance of early detection and therapeutic interventions. Strategies aimed at optimizing blood pressure control, improving endothelial function, and targeting oxidative stress and vascular remodeling are critical to mitigating the systemic consequences of hypertensive microvascular damage and reducing the burden of related cardiovascular and renal diseases.
Coumarins and Hesperetin Inhibit Human Respiratory Syncytial Virus Infection
Authors: de Sá Jéssica Maróstica , Thongpan Ilada , Busso Jefferson de Souza , Rodrigues Thainá dos Santos , Chen Phylip , Helena Alvaro Luiz , Regasini Luis Octavio , Fossey Marcelo Andres , Caruso Ícaro Putinhon , de Souza Fátima Pereira , Peeples Mark Edward
Published: December 2024

Respiratory syncytial virus (RSV) is one of the most prevalent viruses that causes severe acute lower respiratory tract infections (ALRTIs) in the elderly and young children. There is no specific drug to treat RSV, only a broad-spectrum antiviral, ribavirin, which is only used in critical cases. Our research group is investigating antiviral agents of natural origin, such as coumarins and flavonoids, that may help reduce or prevent RSV infection. The cytotoxic concentrations of coumarins and hesperetin were tested on A549 and HEp-2 cells and used in inhibition tests in which 80% of the cells were viable. The anti-RSV action of the molecules was analyzed in A549 and HEp-2 cells and in HBE cell cultures infected with RSV-luc or rgRSV. We also encapsulated the compounds using β-cyclodextrin to improve the permeability and solubility of the molecules. Esculetin and 4-methyl inhibited rgRSV effectively on A549 and HEp-2 cells after 24 hpi, and when they were encapsulated, coumarin, esculetin, and hesperetin presented inhibition against rgRSV in HBE culture. The coumarins inhibit RSV replication in cell culture and even manage to overcome the mucus barriers of the HBE cultures, and β-cyclodextrin was essential for some of the coumarins to enter the cell and therefore to reach their targets.
Online Problem-Based Learning in Child and Adolescent Psychiatry at Nagoya University, Japan
Authors: Kwon Daseul , Kondo Takeshi , Takahashi Noriyuki , Takami Hideki , Nishigori Hiroshi , Aleksic Branko , Bustos-Villalobos Itzel , Yagi Tetsuya , Skokauskas Norbert
Published: December 2024

Purpose: Mental health care is limited worldwide, especially for children and adolescents. Moreover, there are barriers to using mental health services for children and adolescents, including a shortage of standardized training programs and the subsequent workforce. To solve this problem, Japan adopted problem-based learning (PBL) to enhance standardized medical education about child and adolescent psychiatry (CAP). This preliminary study aimed to evaluate online CAP studies at Nagoya University (NU), Japan, with a focus on PBL materials. Design and methods: A descriptive cross-sectional preliminary study was carried out at NU with a questionnaire developed under the Trondheim–Nagoya (TroNA) project by NU and the Norwegian University of Science and Technology (NTNU). The questionnaire, measuring students’ perceptions and understanding of the PBL curriculum and PBL in the CAP, consisted of 15 questions answered on a three-point Likert scale. To compare the PBL CAP delivered online (2021) with the established PBL CAP carried out in person (2019) at NU, an identical questionnaire was used both in 2019 and 2021. Results: The majority of medical students in both in-person (82.5%) and online (91.2%) classes agreed that PBL helped them develop clinical problem-solving skills. In addition, 53.6% of the students in the in-person class and 75.8% of the students in the online class thought PBL enhanced their understanding of CAP. In terms of specific case scenarios, the majority of students in the in-person class (59.8%) and online class (74.7%) agreed that the PBL case helped them understand the importance of a multidisciplinary approach in CAP. Conclusions: Most of the NU medical students were generally satisfied with CAP’s online PBL curriculum. The online PBL CAP curriculum with specific case scenarios can provide medical students with suitable educational means. This preliminary study can to a certain extent provide information relevant for the development of online PBL education in CAP patients and for planning to attract more future doctors to CAP.
Antithrombotic Treatment in Antiphospholipid Syndrome: A Review
Authors: Siniscalchi Carmine , Bernardi Francesca Futura , Di Micco Pierpaolo , Perrella Alessandro , Meschi Tiziana , Trama Ugo
Published: December 2024

Antiphospholipid syndrome (APS) is a thrombo-inflammatory disease propelled by circulating autoantibodies that recognize cell surface phospholipids and phospholipid-binding proteins. APS is an autoimmune disorder associated with recurrent thrombosis of arterial or venous vessels and/or recurrent obstetric complications as miscarriages. APS can be divided into primary or secondary clinical syndrome because of the possible association with other autoimmune systemic diseases as systemic lupus erythematosus (SLE). Vitamin K antagonists remain the mainstay of treatment for most patients with APS and, based on current data, appear superior to the more targeted direct oral anticoagulants. However, the choice of the type of antithrombotic drug is based on the anamnesis of affected patients: patients with previous arterial or venous thrombosis may benefit from anticoagulants, while patients with previous obstetric diseases may benefit from aspirin, but several clinical exceptions may be evaluated. This short review is dedicated to underlining the main clinical evidence for patients affected by APS or CAPS (catastrophic antiphospholipid syndrome) in order to prevent recurrent thrombosis.
Context-Aware QoE for Mobility-Driven Applications Through Dynamic Surveys
Authors: Nawaz Omer , Zhou Yuan , Khatibi Siamak , Fiedler Markus
Published: December 2024

The integration of outdoor smartphone applications with fitness trackers has introduced new opportunities and challenges for user interaction, particularly in mobility-driven activities. While these innovations offer significant benefits, they also pose challenges due to the many factors influencing the user’s quality of experience. Traditional methods of assessing user experiences, such as offline surveys and static questionnaires, often fail to capture the dynamic nature of outdoor activities. This research proposes a novel Quality of Experience (QoE) methodology for mobile applications to enhance the assessment of user experiences in cycling. Focusing on a use case in Blekinge, Sweden, where residents were encouraged to adopt cycling for daily transportation, we extracted land cover data and developed a server-side workflow for bicycle path segmentation. By incorporating dynamic surveys that adapt to users’ real-time experiences, we aim to generate a more accurate and context-aware dataset. This study makes several key contributions: First, it presents a scalable method for bicycle path segmentation; second, it demonstrates the utility and benefits of land cover data extraction; and finally, it evaluates the effectiveness of QoE influence factors through user surveys based on real-world cyclist feedback. This approach is expected to enhance the planning and development of cycling infrastructure by providing urban planners and stakeholders with valuable user insights using adaptable surveys based on route segmentation.
Design and Implementation of an Immersive Web-Based Digital Twin Steam Turbine System for Industrial Training
Authors: Li Zhe , Xiao Hui , Wang Bo , Dong Xuzhu , Shen Lianteng , Di Xiaomeng , Du Xiaodong
Published: December 2024

The steam turbine and its digital electro-hydraulic (DEH) control system constitute vital elements within thermal power generation. However, the complexity of the on-site environment and the high production costs of the equipment hinder users, especially novices, from fully understanding and mastering the operation mechanisms and production processes. In the realm of emerging technologies, the digital twin stands out as a powerful tool for enhancing industrial training and learning for students and operators in this field. This paper details the design and implementation of a web-based digital twin steam turbine system. Initially, a pioneering web-based digital twin architecture is proposed, featuring high-fidelity equipment modeling, web-based immersive 3D displays, algorithm design and networked implementation, and data-driven model synchronization. Subsequently, the functionalities and benefits of the digital twin system in facilitating industrial training are explained, covering aspects such as steam turbine cognitive learning, DEH system simulation learning, and condition monitoring. Finally, a case study in a real thermal power plant is presented to demonstrate the practicability and effectiveness of this web-based digital twin system. This research endeavors to contribute valuable insights and potential solutions to the growing field of web-based digital twin applications in industry.
Enhancing Strength and Corrosion Resistance of Steel-Reinforced Concrete: Performance Evaluation of ICRETE Mineral Additive in Sustainable Concrete Mixes with PFA and GGBS
Authors: V.R Kowshika , Bhaskaran Vijaya , Natarajan Ramkumar , Faridmehr Iman
Published: December 2024

This study investigates the impact of an innovative mineral additive, ICRETE, on steel-reinforced concrete’s compressive strength and corrosion resistance. Nineteen concrete mixes were designed incorporating recycled industrial by-products, including Ground Granulated Blast Furnace Slag (GGBS) and Pulverized Fuel Ash (PFA), with varying dosages of ICRETE. Compressive strength was tested using cube specimens, cured, and assessed at 3, 7, and 28 days following IS 516-2018 standards. Corrosion behavior was evaluated in accordance with ASTM G109, employing macrocell potential monitoring and electrochemical methods, including Tafel extrapolation and linear polarization resistance. The results revealed that ICRETE-enhanced mixes achieved compressive strengths of 56.93 MPa at a water–cement ratio of 0.35 and 50.61 MPa at 0.38, surpassing the control mix’s 50.9 MPa at 0.33. Microstructural analysis via X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed that ICRETE improved hydration, reduced porosity, and refined the microstructure, contributing to more excellent durability. Meanwhile, results demonstrated that the ICRETE additive reduced corrosion rates, displaying lower corrosion current densities and higher polarization resistance values where the corrosion rate dropped from 0.01 mmpy in control samples to 0.0081 mmpy with ICRETE. Environmental assessments indicated that ICRETE could significantly lower CO₂ emissions, reducing up to 46.50 kg CO2 per cubic meter of concrete. These findings highlight ICRETE’s potential to enhance strength and durability, supporting its use in sustainable, eco-friendly concrete applications.
Effectiveness of Video-Game-Based Therapy to Improve Hand Function in Children with Cerebral Palsy: A Systematic Review and Meta-Analysis
Authors: Vezér Mátyás , Gresits Orsolya , Engh Marie Anne , Szabó Bence , Molnár Zsolt , Hegyi Péter , Terebessy Tamás
Published: December 2024

Background: Advances in technology have led to the emergence of new therapeutic methods such as video-game-based therapy (VGBT). This may be a promising new method for improving upper limb function, but the role and proposed uses still need to be clarified. This study aims to investigate the effectiveness of VGBT in children with cerebral palsy (CP) compared to conventional therapy (CT). Methods: A systematic search of five databases was conducted (Cochrane, Embase, Pubmed, Scopus, Web of Science) in January 2024 to identify randomized controlled trials that compared VGBT interventions with CT for children with CP. Our primary outcomes focused on tests on hand functions (HFs) (grip strength, grasp function, manual dexterity tests, hand function questionnaires tests, and upper limb fine motor function tests). A random effects meta-analysis was performed, and ROB2 and GRADE tools were used. Results: Of 2882 articles reviewed, 22 were selected, involving a total of 785 children. Three outcomes were statistically significant in favor of the intervention group: for grasp function, the standardized mean difference (SMD) was 0.80 (95% confidence intervals 0.06, 1.55); for HF questionnaires, the SMD was 0.36 (95% CI 0.04, 0.68); and for HF tests, the SMD was 0.42 (95% CI 0.03, 0.81). The ROB was high risk in two, some concerns for four and low risk in the other cases. The GRADE was moderate in two, while the remaining were low and very low in half of the papers. Conclusions: VGBT has the potential to be an effective tool for rehabilitation of disabled upper limb function in CP as an adjunct to other traditional forms of therapy by integrating it into everyday rehabilitation.
Cerebellar Cavernoma Resection: Case Report with Long-Term Follow-Up
Authors: Toader Corneliu , Serban Matei , Covache-Busuioc Razvan-Adrian , Radoi Mugurel Petrinel , Aljboor Ghaith Saleh Radi , Costin Horia Petre , Corlatescu Antonio Daniel , Glavan Luca-Andrei , Gorgan Radu Mircea
Published: December 2024

Background: Cerebral cavernous malformations (CCMs), particularly when located in the cerebellum, pose unique clinical challenges due to the risk of hemorrhage and proximity to critical neurovascular structures. Surgical resection is often necessary to prevent further neurological deterioration. This case report describes the management of a symptomatic cerebellar cavernoma, emphasizing the use of microsurgical techniques and long-term follow-up. The objective of this study is to illustrate the surgical approach and outcomes of a patient with a hemorrhagic cerebellar cavernoma. Methods: A 63-year-old female presented with vertigo, and neuroimaging revealed a hemorrhagic cavernous malformation located in the right cerebellar hemisphere. Follow-up was conducted at two months and one year postoperatively, with serial imaging to assess lesion recurrence and neurological recovery. Results: Complete resection of the cavernoma was achieved without postoperative neurological deficits. Imaging at two months and one year post-surgery showed no signs of recurrence or new lesion formation. The patient remained asymptomatic, with no cranial nerve deficits or other long-term complications. Conclusions: This case demonstrates the effectiveness of microsurgical resection in treating symptomatic cerebellar cavernomas. The use of advanced intraoperative tools, such as neuronavigation and IONM, contributed to the successful outcome and prevention of postoperative complications. Long-term follow-up remains crucial to monitor for recurrence or the development of de novo lesions.