bluuebunny/crossref_metadata_2025 huggingface dataset:
Principles for the design of a history and heritage game based on the evaluation of immersive virtual reality video games
Authors: Egea-Vivancos Alejandro , Arias-Ferrer Laura
Published: December 2020

The extension of technology in current society has encouraged teachers to introduce products based on VR and IVR in their classrooms, especially video games. In this paper the specific role of video games in cultural heritage and history teaching is analyzed. Moreover, the results of recent research on the application of video games in Secondary Education is discussed. The results advocate taking into account principles such as Civic Education, Historical Relevance, Engagement, Applicability and Multimodality (what has been called the CREAM Model) in the design of a video game. These principles are especially important if the video game aims to introduce archaeological and/or historical contents into the classroom. This model has been implemented in the design of the IVR video game Carthago Nova, which is discussed as an exemplar of how the proposed principles can be engaged. The educational sequence, in which the video game is contextualized, reinforces its educational aims.
Reversible acral and mucosal hyperpigmentation in a patient with B12 deficiency secondary to polyglandular autoimmune syndrome type II
Authors: Asokan Ishan , Wheatley Rachel , Lullo Jenna , Yuen Meiling , Smogorzewski Jan
Published: December 2020

Reversible cutaneous hyperpigmentation often occurs in the setting of nutritional deficiencies and protein energy malnourishment, with atypical presentations arising from autoimmune disease. Here, we present a 52-year-old female with hypertension, type 1 diabetes, and Hashimoto’s thyroiditis, under the diagnosis of polyglandular autoimmune syndrome type II, referred for evaluation of asymptomatic hyperpigmentation of the palms, soles, hard palate, and tongue for 6 months. The patient underwent a significant work-up, including esophagogastroduodenoscopy, which revealed hypertrophic gastropathy as well as evidence of acquired B12 deficiency secondary to pernicious anemia. The patient was initiated on B12 supplementation, with eventual resolution of mucocutaneous findings.
The digital life of the #migrantcaravan: Contextualizing Twitter as a spatial technology
Authors: Walker Margath A , Frimpong Boamah Emmanuel
Published: December 2020

The Central American migrant caravans of 2018 are best understood as having been precipitated by entangled multi-scalar geopolitical histories among the United States, Mexico, Guatemala, Honduras, and El Salvador. Unsurprisingly, the migrants traveling north to the United States garnered widespread attention on social media. So much so that the reaction to the caravan accelerated plans to deploy troops to the US southern border and deny Central Americans the opportunity to seek asylum. This example showcases how the digital world can have exponential material effects. While coverage on border security and migration has been extensive, within political geography, such concerns have rarely been paired with social media. In this article, we take as our object of analysis the digitality or “digital life” of the migrant caravan. Mapping the patterns of migrant caravan-related tweeting paired with the exploration of Twitter’s networked dimensions reveals the platform to be a fundamentally spatial technology. Rather than reflect, refract or distort, Twitter produces and (its power) is in turn produced through spatial mechanisms. We present multiple cartographic visualizations in support of this claim and highlight the ways in which a contextual knowledge of the subject under study—the migrant caravan—can further inform analyses of Big Data.
Narrative metaphor as a tool for exploring character formation
Authors: Roth Donald
Published: December 2020

An assessment of character development naturally entails assessing the narrative sources that a person draws upon in that formative process. I argue that narrative metaphor provides a lens to gain insight into this process. This article lays a theoretical groundwork that connects narrative metaphor to character formation and outlines a range of available Biblical narrative metaphors. It closes by sharing a course assessment that implements this concept and offering some analysis of this assessment to demonstrate how a tool like this can help an institution develop a picture of its students’ character formation.
Comparison of Two Different Criteria for Specific Antibody Deficiency in Patients With Chronic and Recurrent Rhinosinusitis
Authors: Chernikova Diana , Stiehm Richard , Estevez Dennys , Song Charles H.
Published: December 2020

Background

Specific antibody deficiency (SAD) is highly associated with chronic rhinosinusitis (CRS) and is defined by inadequate post-vaccination percentage of protective (≥1.3 ug/mL) pneumococcal antibody serotypes divided by total tested serotypes (post-pPA).

Objective

Although  < 70% post-pPA has been used commonly as the criterion for SAD, we sought to evaluate the clinical outcome of a different definition of SAD.

Methods

203 patients aged 6 to 70 years with CRS were classified, retrospectively by pre-vaccination pPA (pre-pPA) and post-pPA by two different criteria. Using 70% as the threshold for adequate pneumococcal antibody (PA) response, patients were classified as: Group A (adequate pre-pPA), Group B (inadequate pre-pPA, adequate post-pPA), Group C (inadequate pre-pPA, inadequate post-pPA, SAD). Using 50% as the threshold, patients were similarly classified as: Group A’, B’ and C’.

Results

The recurrence rate of sinusitis during the next one year in Group A (pre-pPA ≥70%) was significantly less than that of Group A’ (pre-pPA ≥50%) (10% vs. 34%, P = .03). Group A had lower incidence of sinusitis than Group B (pre-pPA < 70%, post-pPA ≥70%) (10% vs. 34%, P = .025). Among Group B’ patients, the recurrence rate of sinusitis was significantly less among those with post-pPA of ≥70% than those with 50%–69% (28% vs. 69%, P < .01).

Conclusion

Employment of a 70% pPA threshold for SAD in comparison to a 50% threshold would decrease the incidence of sinusitis in the next one year by vaccinating patients in 51–69% pPA range. Pre-existing PAs (Group A) yielded a higher protection against sinusitis than vaccine-acquired antibodies (Group B).
Comparison of 12-Week Additional Effect Features of Formoterol Co-Inhalation and Tulobuterol Patch on Budesonide Inhalation in Elderly Patients With Asthma
Authors: Fukahori Susumu , Kawano Tetsuya , Obase Yasushi , Iriki Jun , Tsuchida-Yabe Tomoko , Tomari Shinya , Fukushima Chizu , Matsuse Hiroto , Mukae Hiroshi
Published: December 2020

Background

For asthma strategy, to avoid the aggravation of bronchial inflammation and contraction, the long acting beta agonist (LABA) addition on inhaled corticosteroids (ICS) has been recommended.

Objectives

To know whether there is any clinical difference between the additional efficacies of Formoterol (FOR) and Tulobuterol (TUL) onto Budesonide (BUD) may be useful for the elderly patients’ asthma treatment strategy.

Methods

Eighteen outpatients with mild to moderate bronchial asthma with FEV1.0% < 80% treated by intermediate ICS dosages visited Respiratory Division of Nagasaki University Hospital or Isahaya General Hospital, Japan Community Health care Organization were subjected, and were randomly assigned (9 cases per group) to either the FBC group (BUD/FOR 160/4.5 µg, 2 inhalations twice daily) or BUD + TUL group (BUD 200 mcg: 2 inhalations twice daily + TUL 2 mg daily) and were compared in parallel with 2 arms for 12 weeks prospectively. Peak expiratory flow, forced expiratory volume in 1 second, impulse oscillometry (IOS), fractional exhaled nitric oxide (FeNO), Asthma Control Questionnaire, mini-Asthma Quality of Life Questionnaire (mini-AQLQ), and occurrence of adverse reactions were compared.

Results

The “Fres” of IOS was improved in FBC group (p = 0.03). The “emotion” domain of mini-AQLQ was improved in BUD + TUL group (p = 0.03).

Conclusion

By changing the drug formulation, the patch was superior in terms of satisfaction, but it was thought that the inhaled combination was superior in improving the respiratory function itself. It is necessary to pay attention to the characteristics of the patient when selecting treatment.
Hydroxyapatite-incorporation improves bone formation on endosseous PEEK implant in canine tibia
Authors: Geng Yuan-ming , Ren Dong-ni , Li Shu-yi , Li Zong-yi , Shen Xiao-qing , Yuan Yu-yu
Published: December 2020

Background:

Poly Ether Ether Ketone (PEEK) has been considered as a potential alternative material for endosseous dental implants, for its low elastic modulus, biocompatibility, and low cost in customized device manufacture. Hydroxyapatite-incorporation is supposed to improve the poor osseointegration of PEEK.

Methods:

In the present study we analyzed the in vivo response of hydroxyapatite-incorporated PEEK (PEEK-HA) implants in canine tibia. PEEK-HA and PEEK implants were implanted and were examined 4 weeks and 12 weeks after implantation with radiology and histology. Commercial titanium dental implants served as controls.

Results:

The ratio of bone volume to tissue volume of PEEK-HA implants was higher than that of PEEK implants 4 weeks after implantation in the μ-CT analysis. The bone implant contact of PEEK and PEEK-HA implants showed no statistical difference in the histological examination, but newly-formed bone around PEEK-HA implants showed more signs of mineralization than that around PEEK implants.

Conclusion:

The study suggested that bone formation was improved with hydroxyapatite-incorporation in PEEK. Hydroxyapatite-incorporated PEEK implants may represent a potential material for endosseous dental implant.
Is it the survival of the fittest or of the jacks-of-all-trades? Business survival in the light of Lazear’s theory
Authors: Mackiewicz Michał , Kurczewska Agnieszka
Published: December 2020

In this article, we apply a proportional hazard model to analyze the determinants of success in a large sample of 1,656 entrepreneurs and former entrepreneurs. We base our research on the theory of entrepreneurship by Edward Lazear, according to which individuals with broad educational and professional experience, in comparison to specialists, are more likely to become entrepreneurs. We extend this theory by verifying whether breadth of education and professional career also contribute to the likelihood of entrepreneurial success. According to our findings, breadth of education not only influences the propensity to start a business but also positively influences the chances of business survival. The breadth of professional experience proved to have a significant impact on business survival, but this result did not hold for extensive managerial experience.

JEL CLASSIFICATION: L26, J24.
Security Environment and Military Spending of Turkey in the 2000s
Authors: Erdağ Ramazan
Published: December 2020

A significant change in Turkey’s foreign policy came with an active engagement in regional issues that, in turn, produced more risks, new areas of interest, regional policy arrangements, and different security challenges in the 2000s. In this vein, Turkey’s military spending has increased to sustain its active policy behavior and goals, both regionally and globally. This article examines Turkey’s military spending between 2000 and 2018 by using Stockholm International Peace Research Institute’s (SIPRI) Military Expenditure Database and its effects on bilateral and regional relations. It argues that the increase in Turkey’s military spending in the 2000s can be explained not only by diversified security threats but also by the quest for autonomy, the desire to be a regional actor, economic capacity increase, efforts to nationalize the defense industry, and the North Atlantic Treaty Organization (NATO) alliance’s inability to form a sufficient umbrella time in the face of security threats, notably in the face of missile threats. The results show that the traditional alliances and strategic cooperation do not guarantee Turkey’s military demands, and its military spending increases, while the proportion of military spending in GDP falls, due to economic growth.
Creation of Superficial Temporal Arteriovenous Loop for Staged Free Tissue Transfer for Dural Coverage in the Setting of Radiation: A Case Report
Authors: Kumbla Pallavi A. , Myers René P.
Published: December 2020

Free tissue transfer for dural coverage can be challenging for various reasons. In the case of malignancy, patients often have received significant doses of radiation to the head and neck leading to fibrosis and osteoradionecrosis. Not only will free tissue transfer need to accommodate an often large defect but will need to protect intracranial contents. Recipient vessel quality and patency is often affected by comorbidities such as diabetes mellitus and coronary artery disease and can be compounded by radiation. Due to these factors, more proximal vessels in the head and neck are often pursued but due to insufficient length, often require vein grafts or arteriovenous loops to reach the donor vessels for anastomosis. This requires larger incisions and harvesting of lengthy veins. In this study, we discuss a technique of harvesting a small dorsal hand vein, that is hidden well in a hand crease, to create an arteriovenous loop between the superficial temporal vessels. Benefits include exploration of the recipient superficial temporal vessels prior to craniectomy without creating additional incisions, readily accessible recipient vessels to reach donor vessels without harvesting lengthy vein grafts, and allowing for arterialization of the superficial temporal vein leading to decreased venous congestion and thrombosis. While this can be done in 1 stage, we perform this in 2 stages to avoid an increased number of anastomoses and increased risk of flap failure. We present the case of an elderly male with multiple comorbidities and scalp osteoradionecrosis secondary to malignancy who this technique was successfully performed on.
CLL dedifferentiation to clonally related myeloid cells
Authors: Dong Qianze , Xiu Yan , Bossler Aaron , Syrbu Sergei , Wang Hongming , Xue Weishuang , Zhao Jinming , Li Qingchang , Jin Meiling , Wang Lili , Boyce Brendan , Sakr Hany , Ansari Mohammad Q. , Zhao Chen
Published: December 2020

Key Points

Common progenitor cells exist in clonally related concomitant chronic lymphocytic leukemia and acute myeloid leukemias. CLL cells dedifferentiated to clonally related myeloid cells posttransplantation.
Identification of CD8+ T cell epitopes through proteasome cleavage site predictions
Authors: Gomez-Perosanz Marta , Ras-Carmona Alvaro , Lafuente Esther M. , Reche Pedro A.
Published: December 2020

Background

We previously introduced PCPS (Proteasome Cleavage Prediction Server), a web-based tool to predict proteasome cleavage sites using n-grams. Here, we evaluated the ability of PCPS immunoproteasome cleavage model to discriminate CD8+ T cell epitopes.

Results

We first assembled an epitope dataset consisting of 844 unique virus-specific CD8+ T cell epitopes and their source proteins. We then analyzed cleavage predictions by PCPS immunoproteasome cleavage model on this dataset and compared them with those provided by a related method implemented by NetChop web server. PCPS was clearly superior to NetChop in term of sensitivity (0.89 vs. 0.79) but somewhat inferior with regard to specificity (0.55 vs. 0.60). Judging by the Mathew’s Correlation Coefficient, PCPS predictions were overall superior to those provided by NetChop (0.46 vs. 0.39). We next analyzed the power of C-terminal cleavage predictions provided by the same PCPS model to discriminate CD8+ T cell epitopes, finding that they could be discriminated from random peptides with an accuracy of 0.74. Following these results, we tuned the PCPS web server to predict CD8+ T cell epitopes and predicted the entire SARS-CoV-2 epitope space.

Conclusions

We report an improved version of PCPS named iPCPS for predicting proteasome cleavage sites and peptides with CD8+ T cell epitope features. iPCPS is available for free public use at https://imed.med.ucm.es/Tools/pcps/.
Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes
Authors: Bai Yang , Wang Rui , Song Wen , Li Lifang , Santoferrara Luciana F. , Hu Xiaozhong
Published: December 2020

Background

The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-monophyletic, but it cannot be revised until more of its species are studied with modern methods.

Results

Here, T. hemispiralis Yin, 1956, T. kiaochowensis Yin, 1956, and T. uruguayensis Balech, 1948, from coastal waters of China, were studied. Lorica and cell features were morphometrically investigated in living and protargol-stained specimens, and sequences of three ribosomal RNA (rRNA) loci were phylogenetically analyzed. The three species show a complex ciliary pattern (with ventral, dorsal, and posterior kineties and right, left, and lateral ciliary fields), but differ in lorica morphology, details of the somatic ciliature and rRNA gene sequences. Tintinnopsis hemispiralis is further distinguished by a ciliary tuft (a ribbon of very long cilia originated from the middle portion of the ventral kinety and extending out of the lorica) and multiple macronuclear nodules. Both T. kiaochowensis and T. uruguayensis have two macronuclear nodules, but differ in the number of somatic kineties and the position of the posterior kinety. Two neotypes are fixed for T. hemispiralis and T. kiaochowensis to stabilize the species names objectively, mainly because of the previous unavailability of type materials. By phylogenetic analysis and comparison with closely-related species, we infer that the ciliary tuft and details such as the commencement of the rightmost kinety in the lateral ciliary field are synapomorphies that may help clarify the systematics of Tintinnopsis-like taxa.

Conclusion

The redescriptions of three poorly known Tintinnopsis species, namely T. hemispiralis, T. kiaochowensis, and T. uruguayensis firstly revealed their ciliary patterns and rRNA sequences. This study expands knowledge and database of tintinnines and helps in identifying potential synapomorphies for future taxonomic rearrangements.
Correction to: Complete genome analysis of a virulent Vibrio scophthalmi strain VSc190401 isolated from diseased marine fish half-smooth tongue sole, Cynoglossus semilaevis
Authors: Zhang Zheng , Yu Yong-xiang , Wang Yin-geng , Liu Xiao , Wang Li-fang , Zhang Hao , Liao Mei-jie , Li Bin
Published: December 2020

An amendment to this paper has been published and can be accessed via the original article.
Asymptomatic malaria and hepatitis B do not influence cytokine responses of persons involved in chronic sedentary activities
Authors: Anabire Nsoh Godwin , Aryee Paul Armah , Ziblim Zulka , Suurbaar Jonathan , Ansah Felix , Helegbe Gideon Kofi
Published: December 2020

Background

Chronic Sedentary lifestyles have been linked to increased odds of stress, elevated anxiety and diminished wellbeing, inducing cytokine production and predispose to hypertension and other cardiovascular diseases. In endemic areas,Plasmodium falciparumand hepatitis B virus (HBV) infections can trigger pro-inflammatory cytokine responses. However, the impact of these infections on cytokine response profiles in individuals engaged in chronic sedentary activities is unknown. This study was aimed at addressing these concerns using a predominantly sedentary population of traders in the Tamale metropolis of Ghana.

Method

Four hundred respondents were categorized, based on their number of working years (< or ≥ 5 years) and number of working hours per day (< or ≥ 10 h), into sedentary (≥5 years + ≥ 10 h)and non-sedentary (≥ 5 years + < 10 h, < 5 years + ≥ 10 h and <  5 years + < 10 h)groups. The participants were tested forP. falciparumand HBV infections using polymerase chain reaction. Blood pressure and cytokines responses were measured. Associations and comparison analysis between variables were determined, and test statistics withp < 0.05were considered statistically significant.

Results

Infection status included: un-infected (93.5%),P. falciparummono-infected (1.0%), HBV mono-infected (3.0%) orP. falciparum/HBV co-infected (2.5%). Majority of the participants, 57.0% (n = 228) were involved in chronic sedentary life style. That notwithstanding, sedentary lifestyle was independent of the infection groups(χ2 = 7.08, p = 0.629).Hypertension was diagnosed in 53.8% of respondents and was independent of infection status(X2 = 6.33, p = 0.097).Pro-inflammatory(TNF-α, IL-1β, IL-6, IL-8 and IL-12)and anti-inflammatory(IL-10, IL-7 and IL-13)cytokine responses were similar among individuals with different sedentary working time and between hypertensive and non-hypertensive individuals(p > 0.05 for all comparisons).Among individuals with different infection status, pro-inflammatory (TNF-α; p = 0.290, IL-1β; p = 0.442, IL-6; p = 0.686, IFN-γ; p = 0.801, IL-8; p = 0.546, IL-12; p = 0.154)and anti-inflammatory (IL-10; p = 0.201, IL-7; p = 0.190, IL-13; p = 0.763)cytokine responses were similar.

Conclusion

Our data suggest that asymptomatic infections ofP. falciparumand HBV together with a high prevalence of hypertension did not have any significant impact on cytokine response profiles among predominantly sedentary traders in the Tamale metropolis of Ghana.
Protocol for iGrow (Infant Growth and Development Study): biopsychosocial predictors of childhood obesity risk at 2 years
Authors: Leerkes Esther M. , Buehler Cheryl , Calkins Susan D. , Shriver Lenka H. , Wideman Laurie
Published: December 2020

Background

Childhood obesity remains a significant public health problem. To date, most research on the causes and correlates of obesity has focused on a small number of direct predictors of obesity rather than testing complex models that address the multifactorial nature of the origins of obesity in early development. We describe the rationale and methods of iGrow (Infant Growth and Development Study) which will test multiple pathways by which (a) prenatal maternal psychobiological risk predicts infant weight gain over the first 6 months of life, and (b) this early weight gain confers risk for obesity at age 2. Infant hormonal and psychobiological risk are proposed mediators from prenatal risk to early weight gain, though these are moderated by early maternal sensitivity and obesogenic feeding practices. In addition, higher maternal sensitivity and lower obesogenic feeding practices are proposed predictors of adaptive child self-regulation in the second year of life, and all three are proposed to buffer/reduce the association between high early infant weight gain and obesity risk at age 2.

Methods

iGrow is a prospective, longitudinal community-based study of 300 diverse mothers and infants to be followed across 5 data waves from pregnancy until children are age 2. Key measures include (a) maternal reports of demographics, stress, well-being, feeding practices and child characteristics and health; (b) direct observation of maternal and infant behavior during feeding, play, and distress-eliciting tasks during which infant heart rate is recorded to derive measures of vagal withdrawal; (c) anthropometric measures of mothers and infants; and (d) assays of maternal prenatal blood and infant saliva and urine. A host of demographic and other potential confounds will be considered as potential covariates in structural equation models that include tests of mediation and moderation. Efforts to mitigate the deleterious effects of COVID-19 on study success are detailed.

Discussion

This study has the potential to inform (1) basic science about early life processes casually related to childhood obesity and (2) development of targeted intervention and prevention approaches that consider mother, infant, and family risks and resources.
Spatial-temporal patterns of malaria incidence in Uganda using HMIS data from 2015 to 2019
Authors: Kigozi Simon P. , Kigozi Ruth N. , Sebuguzi Catherine M. , Cano Jorge , Rutazaana Damian , Opigo Jimmy , Bousema Teun , Yeka Adoke , Gasasira Anne , Sartorius Benn , Pullan Rachel L.
Published: December 2020

Background

As global progress to reduce malaria transmission continues, it is increasingly important to track changes in malaria incidence rather than prevalence. Risk estimates for Africa have largely underutilized available health management information systems (HMIS) data to monitor trends. This study uses national HMIS data, together with environmental and geographical data, to assess spatial-temporal patterns of malaria incidence at facility catchment level in Uganda, over a recent 5-year period.

Methods

Data reported by 3446 health facilities in Uganda, between July 2015 and September 2019, was analysed. To assess the geographic accessibility of the health facilities network, AccessMod was employed to determine a three-hour cost-distance catchment around each facility. Using confirmed malaria cases and total catchment population by facility, an ecological Bayesian conditional autoregressive spatial-temporal Poisson model was fitted to generate monthly posterior incidence rate estimates, adjusted for caregiver education, rainfall, land surface temperature, night-time light (an indicator of urbanicity), and vegetation index.

Results

An estimated 38.8 million (95% Credible Interval [CI]: 37.9–40.9) confirmed cases of malaria occurred over the period, with a national mean monthly incidence rate of 20.4 (95% CI: 19.9–21.5) cases per 1000, ranging from 8.9 (95% CI: 8.7–9.4) to 36.6 (95% CI: 35.7–38.5) across the study period. Strong seasonality was observed, with June–July experiencing highest peaks and February–March the lowest peaks. There was also considerable geographic heterogeneity in incidence, with health facility catchment relative risk during peak transmission months ranging from 0 to 50.5 (95% CI: 49.0–50.8) times higher than national average. Both districts and health facility catchments showed significant positive spatial autocorrelation; health facility catchments had global Moran’s I = 0.3 (p < 0.001) and districts Moran’s I = 0.4 (p < 0.001). Notably, significant clusters of high-risk health facility catchments were concentrated in Acholi, West Nile, Karamoja, and East Central – Busoga regions.

Conclusion

Findings showed clear countrywide spatial-temporal patterns with clustering of malaria risk across districts and health facility catchments within high risk regions, which can facilitate targeting of interventions to those areas at highest risk. Moreover, despite high and perennial transmission, seasonality for malaria incidence highlights the potential for optimal and timely implementation of targeted interventions.
COVID-19: a National Survey on perceived level of knowledge, attitude and practice among frontline healthcare Workers in Nepal
Authors: Tamang Nira , Rai Punam , Dhungana Siddhartha , Sherchan Binod , Shah Bikash , Pyakurel Prajjwal , Rai Saroj
Published: December 2020

Background

The aim of this study was to determine the knowledge, attitude and practice (KAP) regarding the COVID-19 among frontline healthcare workers (F-HCWs) working at different hospitals in Nepal and to identify the factors significantly associated with KAP.

Methods

We used a web-based survey, and a convenience sampling method was adopted to collect data from 603 F-HCWs working at different hospitals in Nepal during the first week of June 2020. A self-administered questionnaire was utilized to assess the KAP perceived by the F-HCWs. It was divided into 4-parts consisting of 30-items, demographic characteristics (10-items), knowledge (10-items), attitude (5-items), and practice (5-items). It consisted of both multiple-choice questions and Likert scale items questionnaire.

Results

Among the participants, 76% reported adequate knowledge, 54.7% reported positive attitude, and 78.9% reported appropriate practice. Statistically significant differences regarding the perceived level of knowledge among F-HCWs were observed among independent variables, including age, gender, level of education, marital status, profession, work experience, source of information, infection prevention and control (IPC) training, and online course(p < 0.05). Similarly, statistically significant differences regarding the attitude among F-HCWs were observed among independent variables, including age, gender, level of education, profession, and online course(p < 0.05). Moreover, only 2-independent variables, including the profession and online course, showed statistically significant differences with practice(p < 0.05). Pearson correlation analysis showed a significant association between knowledge, attitude and practice at the level ofp = 0.01. The factors significantly associated with adequate knowledge were male gender, nurse and doctor, websites and IPC training. Similarly, factors significantly associated with positive attitude were online course related to COVID-19 only. Moreover, factors significantly associated with appropriate practice were master’s degree or above and online course related to COVID-19.

Conclusions

F-HCWs reported adequate overall knowledge with a positive attitude and adopted the appropriate practice. The experienced F-HCWs with higher education and who received IPC training and online course regarding COVID-19 had better KAP. So, the stakeholders must arrange the educational programs and training for F-HCWs for better preparedness tackling with COVID-19.
Correction to: Behind bars: the burden of being a woman in Brazilian prisons
Authors: de Araújo Priscila França , Kerr Ligia Regina Franco Sansigolo , Kendall Carl , Rutherford George W. , Seal David W. , da Justa Pires Neto Roberto , da Costa Pinheiro Patrícia Neyva , Galvão Marli Teresinha Gimeniz , Araújo Larissa Fortunato , Pinheiro Francisco Marto Leal , da Silva Ana Zaira
Published: December 2020

An amendment to this paper has been published and can be accessed via the original article.
Competition for nutritional resources masks the true frequency of bacterial mutants
Authors: Neves Henrique Iglesias , Machado Gabriella Trombini , Ramos Taíssa Cristina dos Santos , Yang Hyun Mo , Yagil Ezra , Spira Beny
Published: December 2020

Background

It is widely assumed that all mutant microorganisms present in a culture are able to grow and form colonies, provided that they express the features required for selection. Unlike wild-typeEscherichia coli, PHO-constitutive mutants overexpress alkaline phosphatase and hence can hydrolyze glycerol-2-phosphate (G2P) to glycerol and form colonies on plates having G2P as the sole carbon source. These mutations mostly occur in thepstoperon. However, the frequency of PHO-constitutive colonies on the G2P selective plate is exceptionally low.

Results

We show that the rate in which spontaneous PHO-constitutive mutations emerge is about 8.0 × 10−6/generation, a relatively high rate, but the growth of most existing mutants is inhibited by their neighboring wild-type cells. This inhibition is elicited only by non-mutant viable bacteria that can take up and metabolize glycerol formed by the mutants. Evidence indicates that the few mutants that do form colonies derive from microclusters of mutants on the selective plate. A mathematical model that describes the fate of the wild-type and mutant populations under these circumstances supports these results.

Conclusion

This scenario in which neither the wild-type nor the majority of the mutants are able to grow resembles an unavoidable “tragedy of the commons” case which results in the collapse of the majority of the population. Cooperation between rare adjacent mutants enables them to overcome the competition and eventually form mutant colonies. The inhibition of PHO-constitutive mutants provides an example of mutant frequency masked by orders of magnitude due to a competition between mutants and their ancestral wild-type cells. Similar “tragedy of the commons-like” cases may occur in other settings and should be taken into consideration while estimating true mutant frequencies and mutation rates.
Oxidative stress mediates thalidomide-induced pain by targeting peripheral TRPA1 and central TRPV4
Authors: De Logu Francesco , Trevisan Gabriela , Marone Ilaria Maddalena , Coppi Elisabetta , Padilha Dalenogare Diéssica , Titiz Mustafa , Marini Matilde , Landini Lorenzo , Souza Monteiro de Araujo Daniel , Li Puma Simone , Materazzi Serena , De Siena Gaetano , Geppetti Pierangelo , Nassini Romina
Published: December 2020

Background

The mechanism underlying the pain symptoms associated with chemotherapeutic-induced peripheral neuropathy (CIPN) is poorly understood. Transient receptor potential ankyrin 1 (TRPA1), TRP vanilloid 4 (TRPV4), TRPV1, and oxidative stress have been implicated in several rodent models of CIPN-evoked allodynia. Thalidomide causes a painful CIPN in patients via an unknown mechanism. Surprisingly, the pathway responsible for such proalgesic response has not yet been investigated in animal models.

Results

Here, we reveal that a single systemic administration of thalidomide and its derivatives, lenalidomide and pomalidomide, elicits prolonged (~ 35 days) mechanical and cold hypersensitivity in C57BL/6J mouse hind paw. Pharmacological antagonism or genetic deletion studies indicated that both TRPA1 and TRPV4, but not TRPV1, contribute to mechanical allodynia, whereas cold hypersensitivity was entirely due to TRPA1. Thalidomide per se did not stimulate recombinant and constitutive TRPA1 and TRPV4 channels in vitro, which, however, were activated by the oxidative stress byproduct, hydrogen peroxide. Systemic treatment with an antioxidant attenuated mechanical and cold hypersensitivity, and the increase in oxidative stress in hind paw, sciatic nerve, and lumbar spinal cord produced by thalidomide. Notably, central (intrathecal) or peripheral (intraplantar) treatments with channel antagonists or an antioxidant revealed that oxidative stress-dependent activation of peripheral TRPA1 mediates cold allodynia and part of mechanical allodynia. However, oxidative stress-induced activation of central TRPV4 mediated the residual TRPA1-resistant component of mechanical allodynia.

Conclusions

Targeting of peripheral TRPA1 and central TRPV4 may be required to attenuate pain associated with CIPN elicited by thalidomide and related drugs.
Tripeptidyl peptidase I promotes human endometrial epithelial cell adhesive capacity implying a role in receptivity
Authors: Santos Leilani L. , Ling Cheuk Kwan , Dimitriadis Evdokia
Published: December 2020

The endometrium undergoes cyclic remodelling throughout the menstrual cycle in preparation for embryo implantation which occurs in a short window during the mid-secretory phase. It is during this short ‘receptive window’ that the endometrial luminal epithelium acquires adhesive capacity permitting blastocysts firm adhesion to the endometrium to establish pregnancy. Dysregulation in any of these steps can compromise embryo implantation resulting in implantation failure and infertility. Many factors contribute to these processes including TGF-β, LIF, IL-11 and proteases. Tripeptidyl peptidase 1 (TPP1) is a is a lysosomal serine-type protease however the contribution of the TPP1 to receptivity is unknown. We aimed to investigate the role of TPP1 in receptivity in humans.

In the current study, TPP1 was expressed in both epithelial and stromal compartments of the endometrium across the menstrual cycle. Expression was confined to the cytoplasm of luminal and glandular epithelial cells and stromal cells. Staining of mid-secretory endometrial tissues of women with normal fertility and primary unexplained infertility showed reduced immunostaining intensity of TPP1 in luminal epithelial cells of infertile tissues compared to fertile tissues. By contrast, TPP1 levels in glandular epithelial and stromal cells were comparable in both groups in the mid-secretory phase. Inhibition of TPP1 using siRNA compromised HTR8/SVneo (trophoblast cell line) spheroid adhesion on siRNA-transfected Ishikawa cells (endometrial epithelial cell line) in vitro. This impairment was associated with decreased sirtuin 1 (SIRT1), BCL2 and p53 mRNA and unaltered, CD44, CDH1, CDH2, ITGB3, VEGF A, OSTEOPONTIN, MDM2, CASP4, MCL1, MMP2, ARF6, SGK1, HOXA-10, LIF, and LIF receptor gene expression between treatment groups. siRNA knockdown of TPP1 in primary human endometrial stromal cells did not affect decidualization nor the expression of decidualization markers prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1). Taken together, our data strongly suggests a role for TPP1 in endometrial receptivity via its effects on epithelial cell adhesion and suggests reduced levels associated with unexplained infertility may contribute to implantation failure.
Combined effects of continuous exercise and intermittent active interruptions to prolonged sitting on postprandial glucose, insulin, and triglycerides in adults with obesity: a randomized crossover trial
Authors: Wheeler Michael J. , Green Daniel J. , Cerin Ester , Ellis Kathryn A. , Heinonen Ilkka , Lewis Jaye , Naylor Louise H. , Cohen Neale , Larsen Robyn , Dempsey Paddy C. , Kingwell Bronwyn A. , Owen Neville , Dunstan David W.
Published: December 2020

Background

Postprandial glucose, insulin, and triglyceride metabolism is impaired by prolonged sitting, but enhanced by exercise. The aim of this study was to assess the effects of a continuous exercise bout with and without intermittent active interruptions to prolonged sitting on postprandial glucose, insulin, and triglycerides.

Methods

Sedentary adults who were overweight to obese (n = 67; mean age 67 yr SD ± 7; BMI 31.2 kg∙m− 2 SD ± 4.1), completed three conditions: SIT: uninterrupted sitting (8-h, control); EX+SIT: sitting (1-h), moderate-intensity walking (30-min), uninterrupted sitting (6.5-h); EX+BR: sitting (1-h), moderate-intensity walking (30- min), sitting interrupted every 30-min with 3-min of light-intensity walking (6.5 h). Participants consumed standardized breakfast and lunch meals and blood was sampled at 13 time-points.

Results

When compared to SIT, EX+SIT increased total area under the curve (tAUC) for glucose by 2% [0.1–4.1%] and EX+BR by 3% [0.6–4.7%] (all p < 0.05). Compared to SIT, EX+SIT reduced insulin and insulin:glucose ratio tAUC by 18% [11–22%] and 21% [8–33%], respectively; and EX+BR reduced values by 25% [19–31%] and 28% [15–38%], respectively (all p < 0.001 vs SIT, all p < 0.05 EX+SIT-vs-EX+BR). Compared to SIT, EX+BR reduced triglyceride tAUC by 6% [1–10%] (p = 0.01 vs SIT), and compared to EX+SIT, EX+BR reduced this value by 5% [0.1–8.8%] (p = 0.047 vs EX+SIT). The magnitude of reduction in insulin tAUC from SIT-to-EX+BR was greater in those with increased basal insulin resistance. No reduction in triglyceride tAUC from SIT-to-EX+BR was apparent in those with high fasting triglycerides.

Conclusions

Additional reductions in postprandial insulin-glucose dynamics and triglycerides may be achieved by combining exercise with breaks in sitting. Relative to uninterrupted sitting, this strategy may reduce postprandial insulin more in those with high basal insulin resistance, but those with high fasting triglycerides may be resistant to such intervention-induced reductions in triglycerides.

Trial registration

Australia New Zealand Clinical Trials Registry (ACTRN12614000737639).
Human breast milk as source of sphingolipids for newborns: comparison with infant formulas and commercial cow’s milk
Authors: Dei Cas Michele , Paroni Rita , Signorelli Paola , Mirarchi Alessandra , Cerquiglini Laura , Troiani Stefania , Cataldi Samuela , Codini Michela , Beccari Tommaso , Ghidoni Riccardo , Albi Elisabetta
Published: December 2020

Background

In the past two decades, sphingolipids have become increasingly appreciated as bioactive molecules playing important roles in a wide array of pathophysiology mechanisms. Despite advances in the field, sphingolipids as nutrients remain little explored. Today the research is starting to move towards the study of the sphingomyelin content in human breast milk, recommended for feeding infants.

Methods

In the present study, we performed a lipidomic analysis in human breast milk in relation with maternal diet during pregnancy, in infant formulas, and in commercial whole and semi-skimmed milks for adults. Mediterranean, carnivorous and vegetarian diets were considered.

Results

The results showed that total sphingomyelin, ceramide and dihydroceramide species are independent on the diet. Interestingly, the milk sphingolipid composition is species-specific. In fact, infant formulas and commercial milks for adults have a lower level of total sphingomyelin and ceramide content than human breast milk with very different composition of each sphingolipid species.

Conclusions

We conclude that human breast milk is a better source of sphingolipids than infant formulas for baby nutrition with potential implications for the brain development and cognitive functions.
Viruses belonging to Anelloviridae or Circoviridae as a possible cause of chronic fatigue
Authors: Grinde Bjørn
Published: December 2020

Chronic fatigue often starts with an acute viral infection—as witnessed in the case of SARS-CoV-2—but indirect consequences of these infections are presumably the actual cause of the condition. As recently reviewed in this journal, the culprit could be a virus already present in the patient. The review covers several types of viruses, but concludes that the question is still open. The focus is on well known, pathogenic viruses for which there are ample diagnostic tools. I argue that there is one lesser-known group of viruses, the related anello- and circoviruses, which ought to be investigated. More or less everyone harbours at least one strain of these viruses in the blood, while not in the spinal fluid. They normally replicate at a low level, but their activity increases in an immune suppressed host; and there are cases where they do reach the brain. The initial infection could facilitate their access to the brain.
Integrin β3 organizes dendritic complexity of cerebral cortical pyramidal neurons along a tangential gradient
Authors: Swinehart Brian D. , Bland Katherine M. , Holley Z. Logan , Lopuch Andrew J. , Casey Zachary O. , Handwerk Christopher J. , Vidal George S.
Published: December 2020

Dysfunctional dendritic arborization is a key feature of many developmental neurological disorders. Across various human brain regions, basal dendritic complexity is known to increase along a caudal-to-rostral gradient. We recently discovered that basal dendritic complexity of layer II/III cortical pyramidal neurons in the mouse increases along a caudomedial-to-rostrolateral gradient spanning multiple regions, but at the time, no molecules were known to regulate that exquisite pattern. Integrin subunits have been implicated in dendritic development, and the subunit with the strongest associations with autism spectrum disorder and intellectual disability is integrin β3 (Itgb3). In mice, global knockout of Itgb3 leads to autistic-like neuroanatomy and behavior. Here, we tested the hypothesis that Itgb3 is required for increasing dendritic complexity along the recently discovered tangential gradient among layer II/III cortical pyramidal neurons. We targeted a subset of layer II/III cortical pyramidal neurons for Itgb3 loss-of-function via Cre-loxP-mediated excision of Itgb3. We tracked the rostrocaudal and mediolateral position of the targeted neurons and reconstructed their dendritic arbors. In contrast to controls, the basal dendritic complexity of Itgb3 mutant neurons was not related to their cortical position. Basal dendritic complexity of mutant and control neurons differed because of overall changes in branch number across multiple branch orders (primary, secondary, etc.), rather than any changes in the average length at those branch orders. Furthermore, dendritic spine density was related to cortical position in control but not mutant neurons. Thus, the autism susceptibility gene Itgb3 is required for establishing a tangential pattern of basal dendritic complexity among layer II/III cortical pyramidal neurons, suggesting an early role for this molecule in the developing brain.
Oral administration of recombinant Bacillus subtilis spores expressing mutant staphylococcal enterotoxin B provides potent protection against lethal enterotoxin challenge
Authors: Xiong Zhile , Mai Jialiang , Li Fei , Liang Bingshao , Yao Shuwen , Liang Zhuwei , Zhang Chao , Gao Fei , Ai Xiaolan , Wang Jielin , Long Yan , Yang Min , Gong Sitang , Zhou Zhenwen
Published: December 2020

Pathogenicity of Staphylococcus aureus is induced by staphylococcal enterotoxin B (SEB). A mutant form of SEB (mSEB) is immunogenic as well as less toxic. Recombinant mSEB and SEB were expressed in pET28a prokaryotic plasmids. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in mSEB-stimulated macrophages were lower than those in SEB-stimulated macrophages (p < 0.001, p < 0.01 respectively). Using CotC as a fusion protein, we constructed recombinant Bacillus subtilis spores expressing mSEB on the spore surface and evaluated their safety and protective efficacy via mouse models. Oral administration of mSEB-expressing spores increased SEB-specific IgA in feces and SEB-specific IgG1 and IgG2a in the sera, compared with mice in naïve and CotC spore-treated groups (p < 0.001, p < 0.01, p < 0.001 respectively). Six weeks following oral dosing of recombinant spores, significant differences were not found in the serum biochemical indices between the mSEB group and the naïve and CotC groups. Furthermore, oral administration of mSEB spores increased the survival rate by 33.3% in mice intraperitoneally injected with 5 µg of wild-type SEB plus 25 µg lipopolysaccharide (LPS). In summation, recombinant spores stably expressing mSEB were developed, and oral administration of such recombinant spores induced a humoral immune response and provided protection against SEB challenge in mice.
Lightweight feature extraction method for efficient acoustic-based animal recognition in wireless acoustic sensor networks
Authors: Al-Quayed Fatima , Soudani Adel , Al-Ahmadi Saad
Published: December 2020

Wireless acoustic sensor networks represent an attractive solution that can be deployed for animal detection and recognition in a monitored area. A typical configuration for this application would be to transmit the whole acquired audio signal through multi-hop communication to a remote server for recognition. However, continuous data streaming can cause a severe decline in the energy of the sensors, which consequently reduces the network lifetime and questions the viability of the application. An efficient solution to reduce the sensor's radio activity would be to perform the recognition task at the source sensor then to communicate the result to the remote server. This approach is intended to save the energy of the acoustic source sensor and to unload the network from carrying, probably, useless data. However, the validity of this solution depends on the energy efficiency of performing on-sensor detection of a new acoustic event and accurate recognition. In this context, this paper proposes a new scheme for on-sensor energy-efficient acoustic animal recognition based on low-complexity methods for feature extraction using the Haar wavelet transform. This scheme achieves more than 86% in recognition accuracy while saving 71.59% of the sensor energy compared with the transmission of the raw signal.