bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Land Subsidence in Bekasi Regency Due to Excessive Groundwater Exploitation Using Geostudio and Plaxis Applications
Authors: Rumbyarso Yonas Prima Arga
Published: January 2025

Cibatu, a sub-district in South Cikarang, Bekasi Regency, West Java, has a high population density and spans an area of approximately 1,200 hectares. This area faces increased groundwater usage for domestic and industrial purposes through deep wells, which has the potential to cause ongoing land subsidence. This study employed Plaxis and Geostudio software to model and predict the extent of land subsidence. Simulation results indicate that Plaxis predicts greater land subsidence than Geostudio, at 13.44 cm/year with pump usage and 3.91 cm/year without pumps. Conversely, Geostudio predicts subsidence of 12.01 cm/year with pump usage and only 0.022 cm/year without pumps. Pump usage has proven to have a significant impact, particularly at Building A of Pelita Bangsa University, where the risk of land subsidence increases by up to 293.09% with pumps. This study aims to identify groundwater levels, soil layers experiencing the greatest subsidence, the extent of subsidence due to water extraction, and the impact of pump usage on land subsidence in the Kaligawe area. The findings conclude that excessive pump usage significantly increases the rate of land subsidence. Policies supporting sustainable groundwater management are needed to address this issue, such as limiting deep well usage, increasing surface water utilization, and implementing water conservation technologies. Additionally, regular monitoring of groundwater levels and subsidence rates is necessary to prevent further impacts on infrastructure and the environment in South Cikarang.
Application and Prospective of CRISPR-Cas in Cancer Diagnosis and Therapy
Authors: Li Xiaoshu
Published: January 2025

Cancer, a leading cause of mortality worldwide, poses a significant challenge to public health. The advent of CRISPR-Cas systems has introduced a novel and promising approach to overcoming the limitations of conventional cancer therapies. This review delves into the burgeoning role of CRISPR-Cas in the oncology field, highlighting its utility in both cancer diagnosis and therapy. In the diagnostic realm, CRISPR-based technologies, such as DETECTR and SHERLOCK, leverage the non-specific collateral cleavage activity of Cas12 and Cas13 proteins for highly sensitive detection of nucleic acid sequences, offering rapid and sensitive diagnostic capabilities. Therapeutically, CRISPR-Cas systems inactivate oncogenes and enhance treatment outcomes through engineered variants and innovative approaches. Variants such as nCas9 and dCas9 have been engineered to enhance specificity and transcriptional regulation, respectively. Challenges including the need for high specificity to avoid off-target effects, long-term safety assessments and ethical considerations persist, yet ongoing research aims to refine these technologies for enhanced efficacy and safety. Overall, CRISPR-Cas systems herald a new era in precision medicine for cancer patients.
Advances in Tumor Inhibition by Repolarized M2 Macrophages
Authors: Liang Mingyue
Published: January 2025

The tumor microenvironment (TME) plays a crucial role in the occurrence, growth, and metastasis of tumors. Tumor-associated macrophages (TAMs), derived from bone marrow precursor cells, exhibit functional plasticity by polarizing into either pro-tumor M2 or anti-tumor M1 phenotypes depending on the microenvironment. This paper explores the advantages and limitations of targeted drug delivery systems using nanocarriers for the repolarization of M2 macrophages into M1 macrophages within the TME. While passive targeted transport techniques allow drug-carrying nanomedicine to selectively accumulate in tumor regions, the efficacy is often hindered by the host's immune response and the inherent toxicity of certain nanomaterials. This review summarizes the current landscape of nanocarriers, emphasizing the need for future research to explore novel nanomaterials and expand the database of drug delivery systems to optimize therapeutic outcomes. Moreover, the potential of polarized macrophage-targeted therapies remains underexplored, suggesting a critical area for future breakthroughs.
Impact Factors of Adult ADHD and Intervention Strategies
Authors: Pan Yue
Published: January 2025

Adult Attention Deficit Hyperactivity Disorder (ADHD) is a condition that affects an individual's attention, impulse control and emotional regulation and is one of the most common neurodevelopmental disorders in our society today. It usually develops in childhood, but some patients who are undiagnosed or who do not receive immediate intervention in childhood continue to have the condition into adulthood, exacerbating their pre-existing symptoms. Existing studies tend to focus more on childhood ADHD and less on adult ADHD. According to World Health Organization (WHO) epidemiological survey data, the prevalence of ADHD in adults is between 2.6% and 2.8%. Attention deficit hyperactivity disorder in adults has a significant negative impact on the individual, the family and society. ADHD can make the patient unable to concentrate at school or work, cause some learning difficulties, and cause some stress and anxiety to themselves, as well as increasing the burden on families and society. This study will investigate the characteristics of attention deficit disorder, the factors that influence it, and intervention strategies. It is hoped that the results of this study will improve understanding of attention deficit hyperactivity disorder in adults.
Application of Tetrodotoxin for Anesthetic and Pain Management
Authors: Lyu Xing
Published: January 2025

Tetrodotoxin (TTX) is a potent neurotoxin that is predominately sourced from marine animals, which serves a dual role as both a fatal toxin and a potential therapeutic compound. TTX functions by selectively binding to voltage-gated sodium channels (VGSCs), preventing sodium ions from entering nerve cells and thereby blocking action potential formation. While its toxicity can lead to severe outcomes like whole-body paralysis and even death, TTX’s unique mechanism of action creates possibilities for anesthetic use and pain management. Unlike traditional local anesthetics, TTX offers long-lasting effects with a lower risk of cardiac toxicity due to its selective binding. TTX can effectively manage chronic and neuropathic pain, including cancer-related and chemotherapy-induced pain, with relatively mild side effects when used at controlled doses. Combining TTX with drugs like bupivacaine has shown a synergistic impact, significantly extending pain relief duration while reducing potential toxicity. The lacks of an antidote for TTX necessitates strict dosing controls and further research to ensure safety. This research explores the mechanisms and safety considerations of TTX, and most importantly, its emerging applications will be analyzed.
Application of Immunotherapy in the Treatment of Solid Tumors
Authors: Zhang Wenhan
Published: January 2025

Cancer is one of the major diseases that seriously threaten human life and health at present, and the number of deaths due to cancer every year ranks in the top 2 of all diseases. In recent years, the malignant tumor is increasing year by year, so it is necessary to explore new therapeutic methods. Immunotherapy of tumor cells is one of the hot topics in tumor therapy, which aims to destroy tumor cells by activating or regulating the immune system of patients. More and more tumor immunotherapy drugs have been successfully developed and marketed, bringing the hope of long-term survival to patients. However, like other tumor drug therapy, tumor immunotherapy has brought huge benefits to some tumor patients and made people full of expectations, but it also faces many difficulties and challenges in pre-clinical research and clinical application. This article summarizes the application and challenge of immunotherapy in solid tumors.
Current Therapy and Challenges for Neurodegenerative Diseases
Authors: Zhang Wenduo
Published: January 2025

Neurodegenerative diseases are a group of diseases characterized by progressive loss and dysfunction of neurons, including Alzheimer's disease, Parkinson's disease, Huntington's disease, and amyotrophic lateral sclerosis, among others. Neurodegenerative diseases can be studied using epidemiology, which is the science of studying the distribution, effects and determinants of diseases, health conditions and events in a population, as well as countermeasures and measures for the prevention and control of diseases and the promotion of health. The incidence rate of a disease can be obtained by epidemiological studies, which is an indicator of the number of new cases in a population. This indicator is similar to the incidence rate and indicates the severity of the epidemic over a relatively short period of time. The incidence of neurodegenerative diseases increases with age, placing a huge burden on patients and their families. In recent years, with the deepening of research into the mechanisms of neurodegenerative diseases, scientists have gradually revealed the complex biological processes behind these diseases and developed a number of potential treatment strategies. This article will provide an overview of the current state of neurodegenerative disease research, recent advances, therapeutic method, and future challenges.
Breast Cancer Image Classification Based on Attention Mechanisms
Authors: Guo Yuyuan , Duan Sinuo , Wang Fangqi
Published: January 2025

Convolutional neural networks have been frequently utilized in computer-aided diagnosis (CAD). Breast cancer image classification is one of the vital applications of CAD. The purpose of this study was to explore the role of attention mechanism in breast cancer image classification. Specifically, this study introduces attention mechanism into the classical image classification deep learning network and constructs a new breast cancer image classification model. Test results indicate that convolutional neural networks perform better in classification when an attention mechanism is added, and they also perform better in terms of training loss and accuracy.
Small-Molecular Drugs for Alzheimer's Disease
Authors: Deng Yupeng
Published: January 2025

Alzheimer's disease (AD) is predominantly characterized by the accumulation of β-amyloid (Aβ) plaques and the formation of neurofibrillary tangles, which are induced by the hyperphosphorylation of the Tau protein. Furthermore, the pathology of AD extends to include the progressive loss of synapses and neurons, accompanied by reactive gliosis. Mannose sodium capsule is a low molecular weight acidic oligosaccharide compound prepared from Marine brown algae extract. It is an innovative drug independently researched and developed by China with independent intellectual property rights, and has been supported by the national Major Special Project of New Drug Creation and Technology. Among the myriads of pharmacological interventions, Mannatna has emerged as a novel therapeutic agent the drug is the first novel drug targeting the brain-gut axis for the treatment of Alzheimer's disease in China and the world. Behind its development logic is a new understanding of the pathogenesis of Alzheimer's disease. that has notably demonstrated both its clinical safety and efficacy in Phase III randomized controlled trials. Post-marketing clinical studies have convincingly corroborated its effectiveness and safety profile across a diverse range of real-world patient populations.
Mechanism and effect of microplastics toxicity in aquatic system
Authors: Wu Shiqi
Published: January 2025

In recent years, the increasingly serious plastic waste pollution has gradually become a global problem. Microplastics (MPs) as an emerging pollutant has received attention. MPs are toxic to humans and many other organisms, and they can accumulate in organisms and amplify their toxic effects through the food chain, thus causing damage to marine biodiversity. Moreover, the seafood provided by the ocean is an important source of food for human beings, and MPs accumulated in fish and crustaceans may be ingested by human beings and cause damage to the digestive system, immune system, respiratory system, nervous system and reproductive system. With the increasing severity of marine plastic pollution, the effect of MPs on aquatic organisms should be of greater concern. By analyzing in vitro or in vivo experimental studies on microalgae, corals, and fish, the effects of MPs on microalgae photosynthesis, coral growth, and fish growth and reproduction were investigated, as well as the oxidative stress induced by MPs in these organisms. It is hoped that it will help people to understand the harmful effects of MPs on aquatic organisms from these three organisms, so that the toxic effects of MPs can be taken seriously.
Current Status of Oseltamivir Application and Its Future Development Trend
Authors: Wang Yixin
Published: January 2025

Influenza is a common respiratory disease with severe complications that can be fatal in severe cases. Oseltamivir is a drug that suppresses influenza disease, it can effectively reduce the symptoms of influenza patients but there are also some adverse effects that can affect the quality of life of patients and the psychological status of the treatment process.  This article examines the side effects, mechanism of action, trends, and future prospects of oseltamivir, and draws conclusions about the drug's use in treating influenza. It provides direction and reference information for future research on this drug, discusses the limitations of its therapeutic efficacy and the challenges it will face. Liver enzyme levels should be carefully monitored and liver health should be carefully assessed when using oseltamivir to treat illnesses such as influenza. These studies will help provide better options for the treatment of diseases such as influenza. However, this paper has not addressed the limitations of whether oseltamivir has additional adverse effects in patients with hepatic and renal disorders, whether they can take the drug normally, and whether the dosage needs to be reduced compared to normal patients. In addition, even though oseltamivir is very effective against the influenza virus, it is not a perfect solution to all problems. It still has limitations and challenges to overcome in terms of viral suppression. In future studies, a deeper understanding of the mechanism of action, efficacy and safety of oseltamivir is needed to develop better anti-influenza drugs to serve patients.
The Relationship Between Self-Focus and Social Anxiety Disorder
Authors: Ren Jiaxin
Published: January 2025

An examination of the severe effects that social anxiety disorder (SAD) has on people is presented in this paper. Particular attention is paid to the condition's early start and modest heredity. The prevalence of seasonal affective disorder (SAD) is highlighted by the fact that it is the fourth most prevalent mental ailment, and it often occurs in conjunction with other diseases. An increasing level of self-focused attention (SFA) has been connected to an increase in social anxiety, which highlights the significant role that SFA plays in the development of social anxiety disorder (SAD). Symptom reduction treatments that are helpful include cognitive therapy and exposure therapy, both of which target SFA. These interventions are considered as useful techniques for reducing symptoms. When it comes to alleviating symptoms of seasonal affective disorder (SAD), in vivo exposure treatment has been shown to be more effective than virtual reality exposure therapy. The review highlights the need of doing further research in order to determine the most effective therapy options and emphasizes the complexity of the societal costs associated with the treatment of SAD. It is intended that the strategies described in the text for lowering the symptoms of social anxiety via treatments that target self-focused attention may serve as a source of inspiration for intervention programs that are designed to alleviate the symptoms of social anxiety.
BiGRU-Att: Blood Glucose Prediction with Bidirectional Recurrent Neural Networks and Attention Mechanisms
Authors: Zhang Wenshan
Published: January 2025

This paper presents an advanced deep learning model that combines a bidirectional gated recurrent unit (BiGRU) and an attention mechanism for predicting blood glucose levels. The innovation of the model lies in its unique structural design, in which the BiGRU is able to capture the backward and forward dependencies in time series data, while the attention mechanism further enhances the sensitivity of the model to critical time steps, thus improving the accuracy of the prediction. This combination not only exploits the power of BiGRU in processing serial data, but also enables adaptive weighting of important features in the input data through the attention mechanism. We validate the effectiveness of the model through experiments on 10 in silicodatasets generated by the UVA/Padova T1D simulator. On these datasets, the model demonstrated excellent performance with a root mean square error (RMSE) of only 0.0719, a metric that is significantly lower than that of existing techniques, proving the superiority and innovativeness of our model for blood glucose prediction tasks.
Role of SHP2 in Colorectal Cancer Development and anti-Tumor Immunity
Authors: Ni Tiancheng
Published: January 2025

Colorectal cancer (CRC), with the second fatality rate and the third incidence rate, is among the most prevalent malignant tumors worldwide. Therefore, it is an important task to find targeted drugs for colorectal cancer clinic therapy. Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) encodes SHP2, a non-receptor tyrosine phosphatase, which is widely expressed in a variety of tissues and cells in the human body. In recent years, a number of studies have shown that SHP2 plays an important role in regulating immune cell function and colitis-related colorectal cancer progression. With the emergence of SHP2 allosteric inhibitors, SHP2 has become a promising target for the treatment of colorectal cancer patients. This article mainly discusses the structure, function, the regulatory mechanism of SHP2 in the tumor microenvironment (TME) of CRC, and the current state and prospect of SHP2 allosteric inhibitors in the therapy of CRC. With the advancement of clinical trials for various solid tumors, future research will be able to usher in more effective and safe SHP2-targeted therapies, providing more beneficial treatment options for CRC patients.
Applications of Metal Nanoparticles in Healthcare and Food Safety
Authors: Chen Sihua
Published: January 2025

The increased world population has led to a rise in pollution, raising the risk of food contamination and foodborne illness. It is often said that illness enters by the mouth. Food spoilage has always been a great hazard to human health. Therefore, ensuring the safety of food is essential in the pursuit of good health. The advent of nanotechnology has sparked potential in the food industry. Nanotechnology has also shown its ability to reduce cases of foodborne diseases, thereby improving global food security from the root. With the introduction of nanoparticles (NPs), particularly metal NPs like gold and silver, food processing and delivery methods have been further refined. These NPs have demonstrated effective resistance against pathogens. Their antimicrobial abilities play a vital role in nano materials for food packaging. The main mechanism of metal NPs involves their interaction with bacterial cell walls, which enhances cell wall permeability and achieves cell wall damage. At the same time, toxic radicals are released from the NPs and can be dissolved to be absorbed by bacteria. These results in the leakage of cellular contents, inhibiting cell replication, and ultimately, cell death. The application of metal-based NPs significantly contributes to food safety. Therefore, in this work, the applications of various nanomaterials in food safety and healthcare are discussed in detail. This work will contribute to promoting the deep application of nanomaterials in food safety and healthcare.
Histopathological Features Analysis of Sarcoidosis in Different Organs
Authors: Ma Ziyuan
Published: January 2025

Sarcoidosis is a complex inflammatory disease involving many systems and its exact cause is still unknown. The characteristic in histopathology of it is the occurrence of epithelioid cell granulomas. The most common type of lymph node involvement is the lungs and chest has risks of other organs. Common diagnostic methods rely on clinical symptoms, imaging, and histopathology. Histopathology is the microscopic study of tissues affected by a disease and is essential for diagnosing sarcoidosis and differentiating it from other granulomatous disorders. According to different affected organs, the histologic pattern of sarcoidosis is also different. Lungs is the most affected organ. Granulomas are usually found within the interstitium, often with varying degrees of fibrosis. Cutaneous manifestations include a range of lesions such as erythema nodosum and lupus chilblain, and histological test may present dermal granulomas and lymphocytic infiltrates. Although liver involvement usually asymptomatic, its characteristics is a portal with a triple granuloma, occasionally accompanied by mild portal inflammation. This paper aims to investigate the histopathological findings of sarcoidosis in different organs.
Mechanisms of Action and Clinical Applications of β-Blockers
Authors: Wu Kexin
Published: January 2025

β blockers are widely used to treat cardiovascular diseases such as hypertension, coronary artery disease, and heart failure because they inhibit β adrenergic receptors. These drugs reduce heart rate, myocardial contractility, and oxygen demand, which is essential for managing a variety of heart conditions. Despite their therapeutic benefits, recent studies have highlighted their limitations in diagnostic imaging, particularly in myocardial perfusion imaging (MPI), where they may reduce sensitivity by altering blood flow patterns. Current research has made significant progress in understanding the pharmacological effects of β blockers, including their effects on heart rate, blood pressure, and myocardial function. However, optimization of use remains challenging, especially in critical care settings and diagnostic procedures. It is important to note that β blockers may affect the accuracy of stress testing, leading to misdiagnosis of ischemic areas on imaging. This study provides a comprehensive analysis of the mechanism of action of β blockers, their clinical applications, and the challenges they pose in diagnostic imaging. The results suggest that while β blockers are effective in reducing cardiovascular mortality, their impact on imaging requires careful management. Temporary discontinuation prior to stress testing may improve diagnostic outcomes, but this approach must be weighed against potential risks. Future research should focus on developing advanced imaging protocols to alleviate diagnostic limitations and explore the potential of novel β blockers that combine β and alphablocking properties. These advancements may improve the efficacy of β blockers while minimizing adverse effects, thereby providing better patient outcomes.
Bioinformatic Approaches in Neurodegenerative Diseases
Authors: Liu Zhuozhi
Published: January 2025

Neurodegenerative diseases (NDDs), including Alzheimer's and Parkinson's, affect the central nervous system, causing progressive loss of cognitive and motor functions. As the global population ages, the prevalence of NDDs rises, imposing significant burdens on patients, families, and society. Despite extensive research, the diagnosis and treatment of NDDs remain challenging due to the complexity of their pathological mechanisms. Traditional methods, such as experimental biology and clinical observation, offer insights but are limited in scope. Bioinformatics, an interdisciplinary field combining biology, computer science, and information technology, provides new opportunities for advancing NDD research. It facilitates the analysis of genomics and proteomics data, aids in drug discovery, and enhances the construction of disease models. This study explores the applications of bioinformatics in NDD research, highlighting its role in identifying disease-associated genes, understanding molecular mechanisms, and discovering potential therapies. Case studies demonstrate how bioinformatics tools can improve research outcomes. The study concludes with a discussion on future research directions and the potential of bioinformatics to develop novel diagnostic and therapeutic approaches for NDDs. Continued advancements in bioinformatics and interdisciplinary collaboration are expected to deepen our understanding of these diseases and lead to more effective treatments.
Recent Development of Cancer Vaccines
Authors: Dong Yanwen
Published: January 2025

Immunotherapy, a truly innovative method in the field of cancer treatment, has attracted a great deal of attention because of its potential to bring about a revolution in the outcomes of cancer therapies. Even though there are many different ways being studied to make immunotherapy even more effective, fully realizing its potential, especially when it comes to cancer vaccines, is still a difficult goal to achieve. This review goes into detail about the different types of cancer vaccines, explaining the basic biological processes behind them and how they work with the immune system to fight cancer. We divide cancer vaccines into three main groups: virus-like particle (VLP) vaccines, peptide vaccines, and DNA/mRNA vaccines. Furthermore, this review also covers how these vaccines are being used in a wide range of infectious diseases and cancer types, emphasizing their versatility and the potential positive effects they can have on patients. Additionally, important topics related to the future of cancer vaccination are discussed, such as new ways to store vaccines to keep them effective, ways to reduce safety concerns, and the creation of personalized vaccines that are tailored to each patient's specific needs and cancer characteristics. By facing these challenges head-on and embracing the latest technologies, we hope to fully unlock the power of cancer vaccines, pushing the boundaries of cancer immunotherapy even further.
The Application of RNA Sequencing in Understanding the Molecular Mechanisms and Therapeutic Potential in Amyotrophic Lateral Sclerosis
Authors: Liu Yiyi
Published: January 2025

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by the progressive loss of motor neurons, leading to muscle weakness and paralysis. While significant advances have been made in understanding the pathophysiology of ALS, there is still no curative treatment, and the underlying molecular mechanisms remain incompletely understood. RNA sequencing (RNA-seq) has emerged as a powerful tool to analyze the transcriptome, providing critical insights into gene expression changes, alternative splicing events, and RNA metabolism. This review highlights the application of RNA-seq in ALS research, including its use in identifying ALS-related gene mutations and differential gene expressions. Key findings include the role of C9orf72, SOD1, FUS, TARDBP, and UBQLN2 mutations in disease progression, as well as the identification of potential biomarkers in cerebrospinal fluid and peripheral blood samples. Additionally, novel RNA-seq technologies, such as single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics, are explored for their ability to capture the heterogeneity of cellular populations and unravel the complex molecular mechanisms underlying ALS. Despite the promise of RNA-seq, several technical, analytical, and ethical challenges must be addressed to fully realize its potential in ALS research. Future directions include integrating RNA-seq data with other multi-omics approaches, advancing personalized medicine, and applying emerging sequencing technologies. Overall, RNA-seq continues to play a pivotal role in uncovering the molecular mechanisms of ALS and holds promise for the development of targeted therapies.
Genetics and Environmental Causes of Lung Cancer: Insights into KRAS, ALK, EGFR Mutations and the influence of Tobacco Smoking
Authors: Hu Jiarui
Published: January 2025

As a critical worldwide health issue, Lung cancer represents one of the leading causes of cancer-related mortality worldwide. Grasping the root causes of lung cancer is essential for creating and utilizing effective treatment strategies. This paper will explore four primary causes in-depth: tobacco smoking, KRAS mutation, ALK mutation, and EGFR mutation. Tobacco smoking, the most prevalent cause, introduces carcinogens that damage lung tissue, leading to cellular mutations. The KRAS mutation, found in about 25% of lung adenocarcinomas, results in uncontrolled cell growth and division by activating oncogenic pathways. The ALK mutation, present in approximately 5% of non-small cell lung cancers (NSCLC), involves a genetic rearrangement, creating a fusion gene that drives malignant cell proliferation. The GFR mutation, found in 10-15% of NSCLC cases, leads to uncontrollable and continuous Epidermal growth receptor activation, promoting uncontrolled cell division. Understanding these causes allows for specific therapies that target each cause to be formed, underpinning the development of diagnostic and therapeutic approaches, which offers hope for more personalized and effective treatments. This paper will also briefly discuss the current treatments for lung cancer, including targeted therapies for each cause of lung cancer and ongoing research aimed at improving patient outcomes.
Surface Plasmon Resonance Biosensors: Advances, Applications, and Future Prospects
Authors: Gao Tony Victor
Published: January 2025

In recent years, surface plasmon resonance (SPR) biosensors have gained importance as analytical methods for biomolecular interaction studies in real-time and without the need for labels. This review encompasses the principle of SPR, the kinds of SPR biosensors, and their diverse applications ranging from medical diagnostics, environmental monitoring, food safety, and biotechnological researches. Recent advances in the development of SPR-based biosensors, including their multiple abilities and portable SPR devices, are also discussed. Furthermore, the discussion focuses on current challenges to SPR biosensors with regard to sensitivity and specificity. Besides, the future directions of research and development are also discussed, which include the possibility of novel enzyme-based SPR biosensors for direct biomarker detection. These advances clearly demonstrate that SPR biosensors, which revolutionize modern detection and monitoring techniques, are becoming increasingly significant in a number of scientific and clinical applications. As SPR technology continues to evolve, its potential to contribute to emerging areas, such as personalized medicine and environmental sustainability, is becoming increasingly apparent.
Methodological exploration of using gene editing technology to study gene function and disease mechanism
Authors: Huang Huaping
Published: January 2025

This paper discusses the application of gene editing technology, especially CRISPR-Cas9 system, in the study of gene function and disease mechanism. By modifying the genome sequence at a fixed point, gene editing technology uses specific nucleases to induce double strand breaks, trigger DNA repair mechanism, and realize accurate gene modification. This paper introduces the working principle of CRISPR-Cas9 and its application in establishing gene knock-out and knock-in models, and emphasizes the importance of this technology in revealing gene function and molecular mechanism of diseases. However, gene editing technology also faces challenges such as off-target effect and technical accuracy, and involves ethical issues. Therefore, the article puts forward that we should strengthen ethical review, improve laws and regulations, and enhance public awareness. In a word, gene editing technology provides a powerful tool for life science and medical research, and shows a broad application prospect.
STRATEGI PENINGKATAN RESPONSIVITAS CUSTOMER SUPPORT DI PT CLOUD HOSTING INDONESIA UNTUK KEPUASAN PELANGGAN
Authors: Ika Ika , Kamdan Kamdan
Published: January 2025

This study aims to analyze strategies for improving the responsiveness of customer support services at PT Cloud Hosting Indonesia to enhance customer satisfaction. Methods include qualitative analysis with data gathered through interviews, observations, and surveys. Results show the effectiveness of implementing AI-based chatbots, structured employee training, and integrated ticketing systems in reducing response times and increasing customer satisfaction. This study provides actionable insights for improving customer support in the cloud hosting industry.
The January 2025 Southern California Wildfires: Public Mental Health Impacts and a Call to Action
Authors: Marthoenis
Published: January 2025

AbstractThe January 2025 Southern California wildfires, among the most severe in recent years, devastated over 40,000 acres, displacing thousands and causing extensive physical and emotional tolls. Beyond the evident physical and economic impacts, the mental health consequences demand urgent attention. This editorial highlights the psychological repercussions, including acute stress, PTSD, anxiety, depression, and substance use disorders, affecting survivors, first responders, and vulnerable populations such as children, the elderly, and low-income communities. Prolonged disruptions and repeated wildfire exposures exacerbate these challenges. The editorial emphasizes the necessity of integrating mental health into disaster response and preparedness strategies, advocating for immediate mental health support, long-term care, and resilience-building within communities. By recognizing mental health as a vital component of disaster management and linking it to climate change policies, a comprehensive approach can mitigate the mental health burden of wildfires and enhance societal resilience to future climate-related disasters.
Epistemologi Irfani Dalam Tradisi Pemikiran Islam
Authors: Ma'rifah Imroatul , Sudirman Sudirman
Published: January 2025

Pada masa dominasi positivisme dalam ilmu pengetahuan, pendekatan irfani dianggap kurang dapat diterima. Namun, perkembangan filsafat ilmu modern, seperti gagasan Paul Feyerabend, membuka peluang bagi berbagai metode untuk digunakan. Dalam penelitian ini, pendekatan kajian literatur dipilih sebagai metode utama oleh peneliti. Irfani dapat dicapai oleh siapa saja yang menjalani perjalanan spiritual. Lebih dari sekadar pendekatan epistemologis, irfani merupakan pengalaman batin yang mendalam, yang dianggap sebagai bagian penting dari kekayaan spiritual Islam dan perlu dihargai untuk memperbaiki praktik keislaman itu sendiri. Melalui metode irfani, individu didorong untuk mendekatkan diri kepada Allah Yang Maha Benar (al-Haqq). Realitas bukan sekadar sesuatu yang ditemukan, melainkan sesuatu yang dicapai. Pemahaman tentang realitas tercapai ketika para ‘arif melakukan perjalanan spiritual untuk mendekat kepada Yang Mahabenar, baik melalui cara ittihad dan hulul, maupun melalui maqom mukasyafah (penyingkapan) terhadap hakikat-Nya. Dengan membersihkan jiwa, para ‘arif memperoleh pemahaman tentang-Nya, yang kemudian memungkinkan mereka untuk mengerti realitas yang dapat dijangkau oleh panca indera dan nalar. Dalam tradisi pemikiran Islam, epistemologi irfani adalah studi tentang pengetahuan yang diperoleh melalui pengalaman spiritual atau batiniah yang mendalam, dengan fokus pada makrifah atau pencerahan sebagai bentuk pengetahuan tertinggi. Tujuan dari artikel ini adalah untuk mengkaji konsep epistemologi irfani dalam konteks tradisi pemikiran Islam, menggali teori-teori utama, dan menyoroti kontribusi tokoh-tokoh penting dalam pengembangannya, seperti Ibn Arabi, Al-Ghazali, dan Jalaluddin Rumi. Epistemologi irfani berbeda dengan epistemologi rasionalis dan empiris karena bergantung pada pengalaman mistik dan intuisi yang melampaui batasan logika dan indera manusia. Epistemologi irfani menawarkan pendekatan komprehensif dalam memahami apa itu pengetahuan, Tuhan, dan eksistensi manusia dengan mengintegrasikan perspektif rasional dan spiritual.
Pengaruh Kualitas Pelaporan Keuangan Terhadap Kinerja Perusahaan Mikro, Kecil, dan Menengah (UMKM)
Authors: Diah Retno Wulansari
Published: January 2025

Pertumbuhan ekonomi negara juga dibantu oleh usaha mikro, kecil, dan menengah (UMKM). Penyerapan tenaga kerja adalah salah satu peran UMKM dalam meningkatkan ekonomi. Upaya untuk membangun UMKM menghadapi banyak tantangan, salah satunya adalah keterbatasan sumber daya keuangan dan informasi. Penelitian ini bertujuan untuk mengetahui kinerja usaha mikro, kecil dan menengah (UMKM) di Kecamatan Tanggetada Kabupaten Kolaka yang dipengaruhi oleh kualitas pelaporan keuangan. Dalam penelitian ini, responden berjumlah 36 UMKM, yang diperoleh melalui metode sampling bola salju. Analisis data yaitu regeresi linear berganda. Hasil pnelitian yaitu kualitas pelaporan keuangan mempengaruhi kinerja bisnis UMKM di Kecamatan Tanggetada Kabupaten Kolaka secara signifikan, dengan nilai t hitung 6,249 yang lebih besar dari nilai t tabel 1,691. Nilai signifikansi kurang dari 0,05 yaitu 0,000. Peneliti merekomendasikan bahwa pelaku UMKM harus meningkatkan kinerja bisnis mereka karena kinerja bisnis mereka akan berdampak besar pada pengembangan.
Analisis Kesulitan Membaca dan Menulis Pada Siswa Sekolah Dasar
Authors: Camilia Ghina Fahmi , Ahmad Arifin , Angelika Welianti , Salsabila Salsabila , Putri Alya
Published: January 2025

Penelitian ini bertujuan untuk mendeskripsikan kesulitan membaca dan menulis siswa kelas tinggi dan faktor yang menghambat pembaca kelas atas. Subjek dalam penelitian ini adalah siswa kelas IV yang beranggotakan sebanyak 30 siswa, dengan 15 siswa perempuan dan 15 siswa laki-laki. Pada penelitian ini, peneliti memfokuskan pada kesulitan dari 1 orang siswa. Dalam penelitian kualitatif, di dalam kelas untuk mengetahui jumlah siswa yang tidak bisa membaca tulis hanya 1 siswa menggunakan teknik wawancara mendalam. Teknik ini melibatkan interaksi antara peneliti dan subjek penelitian untuk mendapatkan pemahaman yang mendalam tentang pengalaman dan pandangan subjek terkait dengan kemampuan membaca tulis. Penelitian dilaksanakan di SD 2 Hadipolo. Penelitian ini menggunakan pendekatan kualitatif, dengan data yang dikumpulkan melalui observasi dan wawancara. Temuan penelitian ini menunjukkan bahwa seorang siswa SD mengalami kesulitan membaca dan menulis. Kesulitan membaca dan menulis yang dialami siswa tersebut disebabkan oleh keterbatasan identifikasi kemampuan dasar membaca seperti huruf, menginterpretasikan bunyi huruf, mengidentifikasi huruf, dan menggabungkan huruf untuk membentuk kata. Kesulitan siswa dalam membaca juga disebabkanoleh kurangnya minat untuk belajar huruf, dorongan untuk membaca, dan bantuan dari lingkungan sekitar, seperti orang tua dan guru.
Effects of topical sodium bicarbonate emulsion on exercise exhaustion and training effects following high-intensity interval training in high-level soccer players
Authors: Meng Shangfu , Zhang Binbin
Published: January 2025

Background and Goal: High-level football players’ competitive performance and training efficacy are significantly impacted by sports weariness. Verifying the possible use of external sodium bicarbonate lotion in professional sports and investigating its effects on the training effect and sports fatigue of elite football players following high-intensity interval training (HIIT) are the goals of this study. Methods: 14 male football players competing at the national level were split into two groups, one experimental and one control, each consisting of seven participants. This study was conducted using a randomized double-blind controlled trial. Twenty min prior to training, the experimental group applied external sodium bicarbonate lotion, while the control group applied a placebo lotion. Group antagonistic training and a seven-day HIIT comprise the training regimen. Assess the effects of the intervention using a variety of multifaceted data, including exercise performance, training impact intensity (TRIMP), delayed onset muscle soreness (DOMS), subjective tiredness scale, perceived fatigue level (RPE), blood biochemical indicators, and heart rate monitoring. Result: Following a single HIIT training session, the experimental group’s blood lactate level was significantly lower than the control group’s (P < 0.05); however, no other indicators showed any significant changes. The experimental group’s TRIMP values were considerably greater than the control group’s during the seven-day training period, and their average DOMS and RPE values were significantly lower (P < 0.05). Maximum heart rate and anaerobic training effect were statistically significant (P < 0.05), and the experimental group outperformed the control group in terms of maximum EPOC, maximum heart rate, anaerobic training effect, and energy consumption. In conclusion, applying sodium bicarbonate lotion externally can improve athletes’ overall performance, increase training stimulus, and considerably lessen muscle soreness and exercise fatigue during high-intensity interval training.
Effects of lumbar cerebrospinal fluid drainage on infection, rebleeding, clinical vasospasm, cerebral infarction, hydrocephalus and mortality among patients with aneurismal subarachnoid hemorrhage
Authors: Chen Qizuan , Zhang Shangming , Pei Jiasheng
Published: January 2025

Lumbar drainage (LD) has been proposed as a treatment to remove blood from the subarachnoid space in subarachnoid hemorrhage (SAH), but its effects on outcomes in aneurysmal SAH (aSAH) remain debated. This study analyzes the impact of continuous lumbar cerebrospinal fluid (CSF) drainage on infection, rebleeding, clinical vasospasm, cerebral infarction, hydrocephalus, and mortality in aSAH patients. A systematic search across PubMed, Web of Science, Embase, Elsevier Science Direct, and Springer Link identified 11 studies with 2059 patients for meta-analysis. The study evaluates the association between lumbar CSF drainage and clinical outcomes, considering how changes in intracranial pressure (ICP) and CSF dynamics may influence pathophysiology in aSAH. The pooled odds ratio (OR) for infection, rebleeding, clinical vasospasm, cerebral infarction, hydrocephalus, and mortality in the lumbar drainage group was 1.17 (95% CI: 0.81–1.70; P = 0.39), 0.75 (95% CI: 0.37–1.54; P = 0.44), 0.60 (95% CI: 0.48–0.75; P < 0.00001), 0.48 (95% CI: 0.35–0.64; P < 0.00001), 0.53 (95% CI: 0.22–1.24; P = 0.14), and 0.55 (95% CI: 0.36–0.84; P = 0.005), respectively, compared with the no-lumbar drainage group. Results indicate that lumbar CSF drainage does not significantly affect the occurrence of infection, rebleeding, or hydrocephalus. However, it reduces clinical vasospasm, cerebral infarction, and mortality, likely due to improved ICP management and enhanced cerebral perfusion. These findings suggest that continuous lumbar CSF drainage may benefit aSAH patients by mitigating ischemic injury, but further studies are needed to confirm its broader applicability and long-term effects on outcomes.
Pemberdayaan Ekonomi Petani Madu Hutan Buloh Seuma Trumon
Authors: Hasbaini Hasbaini , Maulina Dian , Saputra Devi Satria , Rusnanda Resky , Mislina Mislina , Afdhal Afdhal , Jannah Dina Miftahul
Published: January 2025

Perguruan tinggi memiliki tanggung jawab dan peran penting di masyarakat yaitu Tri Dharma Perguruan Tinggi. Selain pendidikan dan penelitian, Perguruan tinggi juga dituntut melakukan kewajiban pengabdian kepada masyarakat sebagai bentuk edukasi dan transformasi ilmu pengetahuan secara langsung. Kegiatan ini berupa Pemberdayaan Ekonomi Petani Madu Hutan di Kemukiman Buloh Seuma Trumon, di mana diketahui sebagai kawasan penghasil madu hutan terbaik di Aceh. Selama ini pengelola hasil panen madu hutan masih dikelola secara tradisional, dan tidak terorganisir secara baik. Melalui pengabdian dosen Politeknik Aceh Selatan, telah dibentuk kelompok tani Jaringan Madu Hutan Buloh Seuma (JMHB), dan diketahui titik koordinat sebaran sarang madu hutan JMHB, serta pelatihan pengemasan produk (Packaging) JMHB dengan waktu pengabdian selama 10 hari. Aktivitas tersebut dinilai cukup berhasil dengan ditandainya madu hutan JMHB sebagai salah satu produk unggulan Aceh Selatan. Sehingga kepercayaan masyarakat dan peningkatan taraf ekonomi anggota kelompok JMHB meningkat.
Pelatihan Aplikasi Komputer dan Teknologi Informasi untuk guru SMA Negeri 1 Samadua
Authors: Yunan Amsar , Ilham Dirja Nur , Anugreni Fera , Candra Rudi Arif , Bean Hasbaini , Jannah Dina Miftahul , Saputra Devi Satria
Published: January 2025

Perkembangan teknologi informasi dan komunikasi (TIK) memberi pengaruh terhadap semua bidang, salah satunya di bidang pendidikan. Pelayanan di bidang pendidikan pada umumnya dilakukan dengan menggunakan teknologi komputer. Komputer merupakan bagian dari perkembangan TIK yang memberi dampak positif terhadap proses administrasi di sekolah. Oleh karenan itu, manusia khsusnya yang berprofesi sebagai pendidik dituntut untuk menguasai dan meningkatkan pengetahuan di bidang TIK. Dalam rangka meningkatkan kualitas di bidang pendidikan, banyak langkah yang telah dilakukan pemerintah salah satunya adalah meningkatkan kualitas SDM khususnya guru-guru melalui berbagai pelatihan-pelatihan. Oleh karena itu, Politeknik Aceh Selatan (POLTAS) melaui Program Studi Teknik Komputer akan melakukan pelatihan penggunaan aplikasi komputer dan teknologi informasi untuk membantu mengatasi berbagai masalah yang dihadapi guru-guru. Kegiatan tersebut sebagai bentuk pengabdian dan peran Politeknik Aceh Selatan kepada masyarakat Aceh Selatan khususnya di di SMA Negeri 1 Samadua. Diharapkan setelah mengikuti pelatihan tersebut guru-guru SMA Negeri 1 Samadua bisa meningkatkan kemampuan diri dalam bidang teknologi informasi guna memenuhi kebutuhan secara lebih cepat, tepat sehingga mampu mengerjakan tugas dengan berkapasitas yang jauh lebih besar, efektif, dan efisien.
Pelatihan Kuliah Berbasis Online Teknik Komputer Poltas di Kecamatan Labuhanhaji dimasa Pandemi
Authors: Yunan Amsar , Ilham Dirja Nur , Setiawan Herry , Candra Rudi Arif , Bean Hasbaini , Khairuman Khairuman , Anwar Ihsan , Anugreni Fera
Published: January 2025

Pengabdian kepada masyarakat adalah salah satu Dharma dari Tri Dharma Perguruan Tinggi, disamping dharma pendidikan dan dharma penelitian, dimana Perguruan Tinggi diharapkan ada usaha secara sadar untuk mencegah terjadinya isolasi Perguruan Tinggi dari masyarakat dan lingkungannya, sehingga akan selalu ada keterkaitan atau bahkan kemanunggalan Perguruan Tinggi dan masyarakat. Saat ini dunia sedang tergoncang oleh kasus coronavirus disease atau dikenal dengan istilahcovid-19 sejak akhir tahun lalu. Virus menular yang berasal dari Wuhan, Tiongkok itu telah menyebar hamper keseluruh dunia termasuk negara Indonesia. Ketika Menteri Pendidikan dan Kebudayaan mengeluarkan Surat Edaran No 3 Tahun 2020 tentang pencegahan Covid-19 pada Satuan Pendidikan pada tanggal 9 Maret 2020; surat Edaran Kesehatan No HK.02.01/MENKES/199/2020 pada 12 Maret 2020; dan Surat Edaran Sekjen Kemendikbud No 36603/A.A5/OT/2020 pada 15 Maret 2020, tentang pencegahan virus covid- 19 ini. Pemerintah juga menghimbau agar belajar dan bekerja dari rumah. Lembaga pendidikan Politeknik Aceh Selatan juga menyambut himbauan pemerintah tersebut dengan mengadakankegiatan akademik dari jarak jauh, seperti perkuliahan yang dilakukan secara online pada masa pandemi ini.