bluuebunny/crossref_metadata_2025 huggingface dataset:
The copper content in the blood plasma of children with different types of short stature
Authors: Sorokman Tamila V. , Sokolnyk Snezhana V. , Popelyuk Oleksandra-Maria V. , Bezruk Tetyana O. , Makarova Olena V.
Published: October 2020

The aim: to investigate the copper content in blood serum of children with different short stature types.
Materials and methods: In the first stage, a comprehensive survey of 258 school children aged 7-11 was carried out in Chernivtsi to assess the prevalence of short stature children of school-age. In the second stage, a comparative study of 42 children aged 3 to 15 years with different types of short stature was conducted. Research: anthropometry, determination of growth hormone, TSH, IGF-1, and copper level in blood plasma.
Results: The largest growth rate in children with somatotropic insufficiency, the syndrome of a biologically inactive growth hormone ranged from 4.1 cm/year to 1.6 cm/year, the ones with a family constitutional low-growth – from 5.7 cm/year to 3.3 cm/year. In 57 cases, that makes 1% of children with short stature, the blood plasma level of copper made less and rated 0.89 ± 0.04 μg / ml, which is believed to be lower (pу0,001) the average copper in children of the control group – 1.07 ± 0.02 μg / ml. Indices of copper in blood plasma in children with somatotropic insufficiency were the lowest and averaged 0.75 ± 0.03 μg / l, p<0.05.
Conclusions: The lack of copper in the blood plasma is observed in most children with short stature, which indicates the need for appropriate correction.
Poikilocytosis in the acne vulgaris comorbidity with demodicosis and functional gallbladder disorder
Authors: Melekhovets Oksana K. , Radko Alevtina S. , Orlovskiy Victor F. , Melekhovets Iuirii V. , Kharchenko Tetiana O.
Published: October 2020

The aim of the study was to evaluate the role of poikilocytosis in the acne vulgaris comorbidity with demodicosis and functional gallbladder disorder. Materials and methods: The study included 140 people with a diagnosis of moderate acne: group 1 – patients with AV, group 2 – patients with AV + Demodicosis, group 3- AV + Demodicosis + functional gallbladder disorder (FGD). Biochemical analysis of lipid profile and liver enzymes, bilirubin was performed with semi-automatic analyzer HUMALYZER 3000. Scanning electron microscopy was performed using scanning electron microscope PEI-106I “SELMI”. Ultrasound investigation was carried out by the SonoScape S6Pro US-diagnostic system with 3 – 5 MHz Ultrasound Probe Transducer С354. Results: The most significant changes in blood erythrocytes morphology were observed in patients with comorbidity of acne, demodicosis and FGD with highest increasing of deformed erythrocytes up to 21%, mainly due to echinocytes І, ІІ order. Correlation analysis has revealed a strong direct association r = +0.75 (p < 0.05) between the poikilocytosis and comorbidity of acne vulgaris with demodicosis and FGD. Conclusions: A strong direct association between acne vulgaris comorbid pathology and the increased number of deformation shapes of erythrocytes confirms the impact of FGD on morphological characteristics of erythrocytes and developing of poikilocytosis.
Hygienic signs of diagnostics of early dysmetabolic disorders symptoms
Authors: Poberezhna Nataliya M. , Omelchuk Serhii T. , Pavlovskyi Serhii A. , Pavlovska Maryna O.
Published: October 2020

The aim of the study was to identify certain health features among healthy people that can serve as a risk factor and lead to the further development of metabolic syndrome.
Materials and methods: A total of 79 men, completely health, were interviewed during 2019 at the Center for Health and Longevity Technology (Kyiv) to assess their health and further correction recommendations for identified abnormalities and available changes. The mean age of men was (37.18 ± 0.89) years. Non-invasive methods were used in the study: determination of anthropometric parameters – height, body weight, waist circumference, abdomen, index of ratio of waist-to-waist circumference, body mass index, body shape index. The constituent compositions of the body were performed with an InBody 220 apparatus with impedance analysis. The metabolic profile was evaluated using FITMATE, a portable metabological device. The cardiovascular profile was determined in the CARDIOLAB + HRV system. The obtained digital data were processed by the method of variational statistics using Microsoft Excel 7.0.
Results: It was found that in 44 (54.4%) of the surveyed men, the waist circumference exceeded 94 cm. The largest number of such persons was found among men aged 31-40 years – (29.11% of the surveyed in this group) and persons aged 41-50 years – 21.52%. Fat distribution (waist circumference / hip circumference) averaged (0.83 ± 0.02). The difference in the body shape index between the group of young persons and groups of older men was established. The predisposition to metabolic disorders is established in 49,37% of cases. Determination of metabolic profile revealed a deviation toward an increase in actual metabolism in 15 people and 15 – in the direction of reducing it.
Conclusions: Thus, the results of a thorough examination of healthy men showed that they may have health changes that characterize the early signs of disease risk, and their presence can be detected by simple non-invasive research methods. The doctor’s task is to assign an in-depth study of the risk factors for the development of a particularly common in our time prevalence of a complex multicomponent metabolic syndrome, which includes the symptoms of the defeat of many body systems, in particular, the endocrine, cardiovascular, urinary and other in order to preserve human health and to recognize it as early as possible, prevent it and avoid complications.
Possible role of vitamin d in pathogenesis of lichenoid dermatoses (a review of literature)
Authors: Bilovol Alla M. , Tkachenko Svitlana H. , Havryliuk Oleksandra А. , Berehova Alla А. , Kolhanova Nataliia L. , Kashtan Kateryna P.
Published: October 2020

The aim of the study was search and analysis of the data of review, experimental and clinical scientific and medical publications on the issues of the possible role of VD in pathogenesis of lichenoid dermatoses.
Materials and methods: An analysis of the studying of the scientific and medical literature was shown. Searching was carried out through the PubMed/MEDLINE portal from the databases of the National Center Biotechnology Information, Web of Science Core Collection, U. S. National Library of Medicine, National Institute for Health and Clinical Excellence, as well as the portals «Scientific Electronic Library eLIBRARY.RU», «Russian Science Citation Index (RSCI)» and «Index Copernicus».
Conclusions: The results of studies had convincingly demonstrated that deficiency of VD in the blood, decrease vitamin D receptors activity can lead to development of lichenoid dermatoses.
Assessed taxation in the health care sector in Ukraine and foreign countries: comparative and legal analysis
Authors: Teremetskyi Vladislav I. , Bandurka Oleksandr M. , Dmytrenko Emiliia S. , Atamanchuk Nataliа I. , Kochura Oleksandra O.
Published: October 2020

The authors have defined and revealed the mechanism for collecting personal income tax, value added tax, single tax relating to health care sector in Ukraine and their analogues in other countries. Special attention has been paid to the problems in this field in Ukraine and the ways of their solution have been suggested. It has been proved that the mechanism of legal regulation of assessed taxation in the health care sector in Ukraine needs to be improved. Taking into account international experience, the authors have offered to amend the Tax Code of Ukraine on: the use of tax incentives of state support (USA); progressive assessed taxation of individual income (Spain, Germany, Poland, Slovakia, etc.); value added tax rates and benefits (USA, France, Hungary) and so on.
Reactive hypoglycemia – an interdisciplinary approach of the disease of XXI Century
Authors: Hall Marianna , Walicka Magdalena , Traczyk Iwona
Published: October 2020

Reactive hypoglycemia is characterized by low blood glucose level in non-diabetic patients. It manifests as a syndrome of adrenergic and neuroglycopenic symptoms in the postprandial period, and their resolution occurs after consuming carbohydrates. The etiology of reactive hypoglycemia is not fully understood. It may occur in patients after gastrointestinal surgery due to too fast gastric emptying. Decreases in postprandial glucose are also observed in people with a pre-diabetes condition in which insulin secretion is disturbed. Hypoglycaemia can also be seen in patients with insulin resistance and hyperinsulinism.
The aim of this study was to summarize existing knowledge about reactive hypoglycemia – etiology, diagnostic model and treatment.
Dynamics of civil law relations in psychiatry
Authors: Savchenko Viktor O.
Published: October 2020

The article deals with the problems of the dynamics of civil law relations in psychiatry. Special attention is paid to the order and peculiarities of emerging, developing and terminating civil law relations in psychiatry. The author conducted a research of normative-legal acts and scientific doctrine on legal regulation issues in psychiatry. The article considers the grounds for providing psychiatric medical services, the order of compulsory and voluntary treatment of psychiatric illnesses, describes the situation when a patient can refuse from psychiatric assistance. The author suggests division of patients, requiring psychiatric assistance, into 4 groups., consideres the order of registrating the patient’s refusal from psychiatric help.
An analysis of early morning acth levels in the first case of pembrolizumab-induced adrenalitis as a delayed immune-related event (dire) – case study
Authors: Harsch Igor Alexander , Gritsaenko Anastasiia , Konturek Peter Christopher
Published: October 2020

The aim: The levels of adrenocorticotrophic hormone (ACTH) are elevated in primary adrenal failure (Addison’s disease) with a peak in the early morning hours. This also occurs under hydrocortisone replacement therapy due to the unphysiological substitution regime. The aim was to study ACTH levels under two different replacement regimens. This is exemplified in a patient with adrenalitis after immunotherapy for malignant melanoma (MM), since (elevated) levels of ACTH and its cleavage product alpha-melanocyte stimulating hormone (α-MSH) raise concerns since receptors for both hormones can be expressed in melanoma cells.
Material and methods: A female with MM had immunotherapy with pembrolizumab and developed adrenalitis with Addison crisis about one year after discontinuation of this therapy (delayed immune-related event = DIRE). ACTH levels were measured hourly (4-8 a.m.) during a “conventional” hydrocortisone replacement therapy and during a therapy with dual-release hydrocortisone.
Results: Salient differences between the morning ACTH profiles under the “conventional” hydrocortisone replacement regimen with 10-5-5 mg/die compared to the single-dose regimen with 20 mg dual-release hydrocortisone were not discernible.
Conclusion: DIRE could be an underestimated problem in immunotherapy and could put the patients at hazard. Especially in case of an endocrinological DIRE concerning the adrenocorticotrophic axis, life-threatening situations can arise for the patients. As for the special situation with M. Addison and MM, where hormonal feedback mechanisms may cause further problems beyond the normal hormonal replacement therapy, we observed no salient differences in the early morning ACTH profiles under different hydrocortisone replacement regimens.
Periodic syndrome associated with the mutation of the receptor gene of the tumor necrosis factor (clinical case)
Authors: Ryznychuk Mariana A. , Lastivka Iryna V. , Pishak Vasily P , Kryvchanska Mariana I. , Khomenko Violeta G.
Published: October 2020

The paper deals with a case of TRAPS in a Ukrainian family. The manifestations of this syndrome appeared at the age of 2,5 years and gradually the attacks of fever became more frequent and the recurrence was typical of this diagnosis. Classically, besides fever, there was an intense abdominal pain, such as an “acute abdomen”, arthralgia in the right hip and headache. Micropoliadenia was also detected. This patient did not have any other symptoms. A genetic study found a mutation in the TNFRSF1A gene (substitution in exon 4 with 3449T> G: p.C117G). This mutation has not been recorded in the international electronic database INFEVERS. The child was administered pathogenetic therapy with a selective blocker of interleukin (IL-1) receptors (anakinra) at a dose of 1-5 mg / kg of body weight subcutaneously daily. After the first injection of anakinra the patient got rid of fever, joint syndrome and of abdominal pain. After 1 week of therapy, laboratory parameters of the disease activity (ESR, CRP) became normal. The child has taken anakinra for two years, there were no exacerbations of the disease or side effects due to the treatment.
The variety of clinical manifestations of congenital periodic fever and the presence of previously unknown genetic mutations that lead to the development of auto-inflammatory syndromes, indicate the need for a detailed study of these diseases.
COGNITIVE DISORDERS IN PERSONS OF WORKING AGE
WITH DYSCIRCULATORY ENCEPHALOPATHY
Authors: Litvin Olena V. , Chernii TetianaV. , Kovalenko Olga Ye. , Klymenko Liliana V. , Perederii Nina O.
Published: October 2020

The aim of the study was to study the neuropsychological features of cerebrovascular disorders in persons of working age at the outpatient clinic stage.
Materials and methods: 90 persons of working age were surveyed. Clinical neurological and clinical instrumental examination.
Results: The first group consisted of patients with dyscirculatory encephalopathy on the background of arterial hypertension – 60 people, the second with dyscirculatory
encephalopathy on the background of cerebral atherosclerosis – 30. Assessment of cognitive function was examined using the MMSE Mental Status Scale, Anxiety, and Depression
Scale using the DASS-21 scale.
Conclusions: According to the results of the study, significant memory impairment on the MMSE scale (p <0.003) was found in patients with dyscirculatory encephalopathy
without arterial hypertension. In patients with dyscirculatory encephalopathy on the background of hypertension, anxiety (p <0.001) and depressive disorders (p = 0.033)
were significantly more prevalent compared with patients with dyscirculatory encephalopathy without arterial hypertension. The increase in depressive disorders and anxiety
in patients with dyscirculatory encephalopathy on the background of arterial hypertension decreases orientation and memory.
SOCIOLOGICAL STUDY RESULTS OF SELF-ASSESSMENT
POSSIBILITIES FOR SELF-REALIZATION AMONG DOCTORS
OF GENERAL PRACTICE – FAMILY MEDICINE IN UKRAINE
Authors: Matiukha Larysa F. , Medvedovska Nataliia V. , Bukhanovska Tetiana M. , Dikhtiarenko Inna Yu.
Published: October 2020

The aim:To study self-assessment opportunities for primary care doctors’ self-realization and their satisfaction with work during health care transformations in Ukraine.
Materials and methods:To achieve this purpose, we compiled a questionnaire according to which an anonymous survey was conducted among 247 family doctors from six
regions of Ukraine (Cherkasy, Zakarpattia, Odessa, Kirovohrad, Sumy, Kiev regions). In the study, we used the following research methods: sociological and medical-statistical.
The method of mathematical statistics by applying Microsoft Excel 2013 was used to analyze research outcomes.
Results:The obtained data on self-assessment opportunities for primary care doctors’ self-realization show that most of the respondents are not satisfied with their work. At
the same time, the older general practitioners – family doctors are, the less important self-realization becomes for them, due to a disturbing fact of a highly probable lack of
job, which is the first priority problem they may face. The willingness to work abroad is reported more than twice as high among family doctors aged up to 47 years. According to
family doctors, irrespective of their age, the priority problems to be solved were reported as follows: poor financings; insufficient material and technical resources of out-patient
clinics of the general medical practice; the necessity of returning national clinical protocols; the incompetence of health management; not clearly established interconnection
between different levels of health care system.
Conclusions:Family doctors in Ukraine have mostly unsatisfactory conditions for their self-realization. This fact has a significant influence on their willingness to change a job,
especially among doctors of young age.
ANTIPHOSPHOLIPID AND ANTINEUTROPHIL ANTIBODIES LEVELS IN MEN WITH STABLE CORONARY HEART DISEASE AND POSTINFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH THE DISEASE MANIFESTATION
Authors: Nazarova Marina S. , Stanislavchuk Mykola A. , Burdeina Liudmyla V. , Zaichko Nataliia V.
Published: October 2020

The aim is to study the levels of antiphospholipid (aPL) and antineutrophil antibodies in men with stable coronary heart disease (CHD) with postinfarction cardiosclerosis and
to evaluate its relationship with the disease manifestation.
Materials and methods: 164 men with stable CHD and postinfarction cardiosclerosis (53.0 ± 9.14 (M ± σ) years) and 48 age-matched men without CHD were examined.
The total aPL IgG and IgM, beta-2 glycoprotein 1 antibodies (anti-β2-GP 1) IgG and IgM, and antibodies for neutrophil proteinase-3 / myeloperoxidase (anti-PR3 / MPO) IgG
were determined by ELISA.
Results: Positive levels of aPL and anti-β2-GP 1 of IgG were identified in 56.7% (33.5% double positivity of aPL + anti-β2-GP 1) and 29.2% of control group (p < 0.001), while
the IgM was lower (11.6% vs. 6.2%, p = 0.55, respectively). Significantly higher (1.5-1.7 times) levels of aPL and anti-β2-GP 1 were identified in patients who underwent
myocardial infarction (MI) aged less than 44 years, after Q-MI, recurrent MI, in the presence of ischemic stroke, livedo reticularis. In 6.7% of patients with positive levels of aPL
and anti-β2-GP 1 low IgG anti-PR3 / MPO levels were detected.
Conclusions: In men with postinfarction cardiosclerosis, IgG positivity according to total aPL and anti-β2-GP 1 is associated with a higher incidence of Q-MI and with recurrent
MI. Men with postinfarction cardiosclerosis have a tendency to increase anti-PR3 / MPO levels of IgG under conditions of double aPL positivity and anti-β2-GP1 of IgG.
THE USE OF PHOTODYNAMIC THERAPY IN THE TREATMENT OF DENTAL CARIES IN CHILDREN OF CONTAMINATED AREAS OF THE ECOSYSTEM OF THE UPPER TYSA REGION
Authors: Potapchuk Anatoliy M. , Almashi Vasyl M. , Lomnitsky Igor Ya. , Rusyn Vitaliy V. , Hegedush Viktoria
Published: October 2020

The aim: improving the effectiveness of treatment of dental caries in children using the method of photodynamic therapy.
Materials and methods: The treatment of permanent tooth decay was performed in 35 children of the Upper Tysa region aged 12-15 years. Using the polymerase chain
reaction method, the clinical efficacy of photodynamic therapy in the treatment of dentin caries was determined.
Results: During the study, by PCR analysis, dentin caries most commonly revealed genetic markers of DNA of the five most virulent anaerobic bacteria: Prevotella intermedia,
Fusobacterium spp., Enterococcus Faecalis, Veilonella spp., Candida albicans. After treatment of the carious cavity by the method of photodynamic therapy with exposure of
30 s – fusobacterium spp. were not detected, the detection rate of prevotella intermedia decreased 3-fold, enterococcus faecalis 3.5-fold, veilonella spp.- 5-fold, and candida
albicans- 8-fold; after treatment with carious cavities with 60 s exposure – anaerobic microorganisms were not detected in the investigated samples. The effect of photodynamic
therapy with laser exposure of 60 seconds on caries-causing streptococci resulted in their total death, and with exposure of 30 seconds – the frequency of isolated strains
decreased several folds.
Conclusions: The use of photodynamic therapy in the treatment of dentine caries is a highly effective and pathogenetically sound method of treatment that provides a significant
reduction in the optional and obligate types of cariesogenic microorganisms.
ULTRASTRUCTURAL FEATURES OF HERPESVIRUS-INDUCED AORTIC INTIMA DAMAGE IN MICE FED THE HIGH-FAT DIET
Authors: Turchyna Nataliia S. , Savosko Serhii I. , Сherenko Tetiana M. , Ribalko Svitlana L. , Starosyla Daria B.
Published: October 2020

The aim: To study the effect of a high-fat diet (HFD) on the structural changes in the aortic intima in intact and HSV-1-infected mice using Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). Materials and methods: In experiments Balb/c mice were infected with the HVS-1 and fed high-fat diet and 12 weeks later aortic ultrastructure was examined by SEM and TEM methods. The animals were subdivided into four experimental groups: 1st group – HSV-1-infected animals; 2nd – animals consuming high-fat diet (HFD); 3rd – infected animals that were subsequently consuming a high-fat diet (HSV / HFD); 4th – animals consuming a high-fat diet that were subsequently infected with HSV-1 (HFD / HSV) (n = 6); and control group – intact animals. Results: HVS-1 impaired ultrastructural changes in aorta greater than high-fat diet and HVS-1 alone (higher density of lipid inclusions in the subendothelial space, necrosis of endothelial cells), and infection of mice after high-fat diet ended 100% mortality. The formation of atheroma in the aortic wall during HFD was not detected, but the initiative manifestations of atherogenesis have been identified and restricted in the aortic intima. These structural changes included lipid inclusions in the subendothelial space, cell damage and destruction, which lead to an increase cellular detritus in the 3rd (HSV / HFD) group. Conclusions: HSV infection potentiates the accumulation of lipid inclusions in the aortic intima during a HFD, facilitates infection and contributes to the development of acute infection.
THE RELATIONSHIP BETWEEN PROLACTIN LEVELS AND THE RESULTS OF HOLTER MONITORING IN PATIENTS WITH STABLE ANGINA OF TENSION AND GASTROESOPHAGEAL REFLUX DISEASE
Authors: Khukhlina Oksana S. , Antoniv Alona A. , Drozd Viktoriia Yu. , Kotsiubiichuk Zoriana Ia. , Smandych Vitaliy S.
Published: October 2020

The aim: Determine the possible relationship between prolactin levels and the results of Holter monitoring in patients with stable angina of tension and gastroesophageal
reflux disease (GERD).
Materials and methods: The study included 118 patients with stable angina of tension of I-II functional class. Of these, 88 patients with stable angina of tension with comorbid
GERD (A, B, C), who were included in the second group of the study and 30 patients with isolated stable angina of tension which formed the 1st group. All patients who were
included in the study prior to treatment were evaluated for serum prolactin levels and Holter monitoring.
Results: In patients with stable angina of tension and GERD, prolactin levels exceed the norm levels and are higher than the level of prolactin in the group with isolated stable
angina (p<0,05), as well as the number of episodes of painful and painless myocardial ischemia and their duration (p<0,05). The presence of a comorbidity with GERD provokes
an increase in the total duration of episodes of ischemia per day in patients with stable angina compared to those in patients with no GERD. A direct linear relationship was
founded between prolactin serum levels and the number of episodes of pain in myocardial ischemia in patients with stable angina and GERD per day, and between the total
duration of episodes of myocardial ischemia and prolactin levels.
Conclusions: In patients with stable angina and GERD, in 70.4% of cases, the level of prolactin exceeds the norm in 2,6 times, and is higher than the level of prolactin in the
isolated group of stable angina. For the comorbidity of stable angina and GERD, the number of episodes of painful, painless myocardial ischemia and their duration is more than
once in the group of patients with stable angina without GERD (p<0.05).
PERIODONTAL STATUS IN PATIENTS WITH DISEASES OF HEPATOBILIARY SYSTEM, BURDENED WITH TOBACCO AND DRUG ADDICTION
Authors: Furdychko Anastasia I. , Ilchyshyn Marta P. , Fedun Irena R. , Barylyak Adriana Ya. , Slaba Oksana M. , Khoroz Lesya M.
Published: October 2020

The aim: To study the effect of drug addiction and smoking on the status of periodontal tissues in patients with hepatobiliary pathology. Materials and methods: 58 smokers, 63 drug addicts with hepatobiliary pathology were examined and 92 persons of comparison group (with inflammatory periodontal diseases affected by hepatobiliary pathology, without addiction). Examination of patients included determination of iodine number by Svrakov, S-L and Stallard hygiene indices, PMA index and index PBI. Results: The results of examination of smokers are worse in patients with cirrhosis than in patients with chronic toxic hepatitis and non-alcoholic steatohepatitis and also the results of these two groups was significantly worse than that of the comparison group (iodine number by Svrakov – 1,4 times and 1,3 times higher, PMA index – 1,7 times and 1,4 times worse, in accordance). The results of the study showed that drug addicts patients are worse values in patients with cirrhosis than in patients with chronic toxic hepatitis and non-alcoholic steatohepatitis and also the results of these two groups was significantly worse than that of the comparison group (iodine number by Svrakov – 1,5 times and 1,4 times higher, PMA index – 1,7 times and 1,5 times worse, in accordance). Conclusions: Presence of hepatobiliary pathology in smokers and drug addicts increases the risk of periodontal diseases.
EFFICACY ASSESSMENT OF THE SCHEME FOR PREVENTION OF HERPESVIRUS INFECTION MANIFESTATIONS IN THE ORAL CAVITY OF PATIENTS WITH HERPES-ASSOCIATED GENERALIZED MODERATE SEVERITY PERIODONTITIS
Authors: Volosovets Tetiana M. , Kravchenko Anna V.
Published: October 2020

The aim: Efficacy assessment of herpesvirus infection drug prophylaxis (HVI) manifestations in the mouth of patients with chronic herpes-associated generalized periodontitis
(CHGP) of moderate severity during and shortly after closed curettage.
Materials and methods: The total of 87 patients with CHGP of moderate severity aged 35-60 years were examined and divided into groups according to the presence of HVI:
Group I (main) included 48 patients who had herpesvirus infection; group II (comparison) – 39 patients who were not found herpesvirus infection. Group III was formed based
on the data of out-patient medical reports retrospective analysis and was used to compare the number of complication cases. The control group included 20 patients with intact
periodontium.
Prior to treatment, patients in group I, was additionally assigned multicomponent phytocomplex. The treatment efficacy was assessed by the concentration dynamics of A, G, M
immunoglobulins, circulating immune complexes (CIC) and sIgA, hygienic and periodontal indices, as well as by the dynamics of gum fluid and the recovery terms.
Results: The study on the dynamics of clinical indices and some humoral immunity indices confirmed the main group patients after closed curettage had positive shifts in sIgA,
IgA, IgG, IgM and CIC levels. Their indices did not have a statistically significant difference with similar indicators of the comparison group. In group I, complications in the form
of HVI manifestations in the buccal mucous membrane (BMM) were found in 8.33% of patients, which had a statistically significant difference (p <0.001) from the percentage
of patients with HVI, with complications in group III (35.71% ). The recovery terms for patients in group I were by 16.7% shorter than the similar terms in group III.
Conclusions: The results obtained indicate that the phytocomplex used by us can be applied as an immunomodulatory agent for the prevention of herpes virus infection
manifestations in the oral cavity of patients with interventions in BMM and periodontal tissues.
PRIORITIES OF ANTI-HYPERGLYCAEMIC DRUG THERAPY IN PATIENTS WITH TYPE 2 DIABETES AND HEART FAILURE
Authors: Rоsul Мaryana М. , Bletskan Мiroslava М. , Ivano Nataliya V. , Korabelschykova Marina O. , Rubtsova Yelyzaveta І.
Published: October 2020

The aim is to explore the possibilities of improving the effectiveness in preventing cardiovascular diseases and heart failure using sodium-glucose co-transporter 2 inhibitors.
Materials and methods: The analysis of the existing clinical and experimental data on the effect of sodium-glucose co-transporter 2 (SGLT-2) inhibitors on the cardiovascular
system, the condition of kidneys, cardiovascular risk factors.
Review: SGLT-2 inhibitors are the first class of glucose-lowering agents in large-scale studies (EMPA-REG OUTCOME, CANVAS, CVD-REAL, CVD-REAL2) which have demonstrated
the ability to improve cardiorenal outcomes and reduce the risk of hospitalization with heart failure in patients with diabetes. In addition to hypoglycaemic action, SGLT-2
inhibitors show a number of pleiotropic effects, which are potentially capable of reducing cardiovascular risk: diuretic effect, decrease in: blood pressure, arterial wall stiffness,
waist and body weight, expression of albuminuria, etc. The use of drugs of this class opens great prospects not only in terms of glycaemic control, but also in the prevention of
cardiovascular complications of diabetes.
Conclusions: 1. When choosing glucose-lowering agents in patients with type 2 diabetes, it is necessary to take into account their impact on the risk of development and the
course of heart failure.
2. SGLT-2 inhibitors ought to be considered as a preferred method of treatment for type 2 diabetes in patients with heart failure or with a risk of heart failure that meets the
latest recommendations of the European and American Diabetes Association.
MORPHOLOGICAL ASSESSMENT OF WOUND HEALING AFTER CRYODESTRUCTION OF SKIN USING AN AQUEOUS COLLOIDAL SOLUTION OF C60 FULLERENES
Authors: Vlasov Oleksandr O. , Kovalov Gennadiy O. , Myroshnychenko Mykhailo S.
Published: October 2020

The aim is to assess the morphological state of a cold skin wound in an experiment, using an aqueous colloidal solution of C60 fullerenes.
Materials and methods: An experimental study was conducted in 30 six-month-old male rats divided into three groups, 10 individuals in each group: group I included rats that did not undergo any manipulations; group II was represented by rats which underwent a cold wound simulation on the lateral thigh surface; group III was represented by rats who had a simulated cold wound followed by introduction of an aqueous colloidal solution of C60 fullerenes at a dose of 0.1 mg/kg for 5 days into the abdominal cavity, starting from the cryodamage day. Animals of all groups were removed from the experiment on days 7, 14 and 21.
The material for the morphological study was skin with underlying soft tissues. The resulting sample was studied, using histological, histochemical, morphometric and statistical research methods.
Results: C60 fullerenes stimulate proliferative activity in the epidermis, located in the wound marginal regions or covering the regenerate surface, which leads to an increase in epithelial layer thickness by 2.6, 2.6, 2.5 times on 7, 14 and 21 days; promotes faster cleansing of the wound from necrotic tissue, reducing the thickness of the zone of primary necrosis on days 7, 14 and 21 by 1.3 times by increasing the demarcation zone on days 7, 14 and 21 by 1.4, 1.4 and 2, 2 times; reduces the thickness of the secondary necrosis area on 7, 14 and 21 days by 1.4, 1.5 and 1.3 times; accelerates filling of the wound defect with granulation tissue, the layer thickness of which increases by 2.3, 2.2 and 1.4 times on the 7th, 14th and 21st days, respectively; reduces the severity of hemodynamic disorders and cell infiltration in the wound defect adjacent tissues of the dermis, hypodermis with the underlying muscle tissue.
Conclusions: A comprehensive morphological study of the experimental material indicates that the injection into the abdominal cavity of an aqueous colloidal solution of C60 fullerenes activates reparative processes in the skin cold wounds, which go through all the classical stages.
INFLUENCE OF LYS198ASN POLYMORPHISM OF ENDOTHELIN-1 GENE ON ISCHEMIC ATHEROTHROMBOTIC STROKE CHARACTERISTICS
Authors: Oleshko Tetiana B. , Chaika Iryna S. , Oleshko Tetiana M. , Harbuzova Viktoriia Yu.
Published: October 2020

The aim: Investigate the effect of Lys198Asn polymorphism of the EDN1 gene on ischemic atherothrombotic stroke characteristics.
Materials and methods: Venous blood of 170 patients with ischemic atherothrombotic stroke (IAS) and 124 patients without cerebrovascular pathology, who made up the control group, used for the study. Lys198Asn (rs5370) polymorphism of the EDN1 gene was determined by the polymerase chain reaction method followed by restriction fragment length analysis. Statistical analysis was performed using SPSS-17.0. The values of Р < 0.05 were considered reliable.
Results: An association between the Lys198Asn polymorphism of the EDN1 gene and the IAS development was detected. For Asn/Asn genotype carriers, the risk of IAS developing is 4 times higher than that of homozygotes for the major allele. The association of this polymorphism with the arterial pool, whose atherothrombotic changes lead to the development of IAS, was found in individuals with BMI < 25 kg/m2. Lys198Asn polymorphism also affects the severity of IAS in persons with hypertension and non-smokers.
Conclusion: The Lys198Asn polymorphism of the EDN1 gene influences some characteristics of ischemic stroke.
LONG-TERM CONSEQUENCES OF PRIMARY PERCUTANEOUS CORONARY INTERVENTION IN PATIENTS WITH ST-ELEVATION MYOCARDIAL INFARCTION
Authors: Izhytska Nataliya V. , Besh Dmytro I. , Besh Olesya M. , Fil Adrian Y.
Published: October 2020

The aim: To investigate long-term effects of primary percutaneous coronary interventions (pPCI) in patients with STEMI basing on the prevalence of clinically relevant endpoints. Materials and methods: Totally 200 patients with STEMI hospitalized within a period of “therapeutic window” for revascularization were included into the study. 100 patients who additionally to pPCI underwent manual thromboaspiration entered the main group. The comparison group consisted of 100 patients who underwent standard pPCI. Results: Six months after the pPCI, the incidence of either major adverse cardiac events and the combined endpoint did not differ in the main and comparative groups (11.8% vs. 12.5%, p = 0.888 and 18.2% vs. 20.8%, p = 0.658, respectively). There were no significant differences in these endpoints taken separately. In twelve months after procedure, also there were no significant differences between the groups. However, a tendency toward lower incidence of chest pain was observed in the main group (p = 0.08) during this period that was lost in 24 months after pPCI. None of these techniques demonstrated significant advantages during the whole duration of the follow-up period. Conclusions: The addition of manual thromboaspiration to the standard pPCI in patients with STEMI and severe thrombosis of the culprit artery did not significantly influence the prognosis.
THE EFFECTIVENESS OF CARDIOVASCULAR EDUCATION IN THE PREVENTION OF HEART DISEASE AMONG THE INHABITANTS OF LESSER POLAND
Authors: Podolec-Szczepara Natalia , Skoczek Adrianna , Szczepara Sylwia , Jakiel Marcin , Lenart-Migdalska Aleksandra , Prochownik Paweł , Libiszewska Natasza , Sarnecka Agnieszka , Knap Klaudia , Komar Monika
Published: October 2020

Introduction: Numerous risk factors for cardiovascular disease (CVD) are modifiable, therefore understanding their effects has a significant impact on lowering the incidence and mortality. The “Małopolska Cardiovascular Preventive Intervention Study (M-CARPI)” aims to educate the inhabitants of the voivodeship regarding preventative measures for developing CVD. The aim: To examine the effectiveness of the seminars regarding preventative measures for developing CVD according to the M-CAPRI among the inhabitants of Lesser Poland. Material and methods: The seminars were carried out by a doctor with a specialization in cardiology, in the form of presentations, based on materials prepared by experts in accordance with European and Polish guidelines. Both initially and after the course, participants completed questionnaires regarding the subjects discussed. Results: The study covered 558 people (137 men, 411 women). The analysis was based on the sub-populations of retirees and non-retirees. The knowledge of risk factors was checked, the respondents most often indicated incorrect judgment of nutrition, physical activity and smoking (45.9%, 39%, 44.2% for non-retirees and 22.8%, 27.6%, 16.5% for retirees, respectively). A significant increase in the number of people who indicated individual risk factors after the training was found (p=0,047 for HDL level and for others). Conclusions: Educating and creating awareness towards the prevention of CVD according to the M-CAPRI survey protocol is an effective tool for prevention. Due to the limited knowledge of cardiovascular risk factors, it is necessary to carry out activities to raise awareness of the importance of cardiological disease prevention. Preventive interventions are equally effective regardless of age or profession.
LUMBAR LORDOSIS ANGLE VALUE ANALYSIS AND BONE TISSUE DENSITY IN THE LS SECTION IN WOMEN AFTER 50 YEARS OLD
Authors: Wojcik Gustaw , Rutkowska Elzbieta , Mysula Ihor , Szepeluk Adam
Published: October 2020

Introduction: The lumbar lordosis angle measured by radiology under normal conditions is between 135-1400. Any deviation from the values is associated with a change in static-dynamic loads and should translate into a change in bone tissue within the lumbar-sacral border, which results from a change in the distribution of load forces acting on individual parts of the vertebral bodies.
The aim: To determine the relationship between individual variants of lordosis (hypolordosis, hyperlordosis, norm) and the general density of the spongy part of individual vertebral bodies and in selected zones of the lumbosacral zone (L5 / S1).
Material and methods: The study group was a population of women over 50 (n = 277), it was divided into three subgroups, i.e. women with: normal, shallow and deepened lordosis. In the sagittal view, the density of vertebral bodies from L1 to S1 was assessed.
Results: The difference in the overall density of individual stems between normal lordosis and extreme values was significant (p≥0.05) only for the L1, L2 and S1 vertebrae, whereas in the case of an excessive lordosis angle, the density of spongy bone tissue showed significant differences for all measurement points.
Conclusions: Deviations from the correct values of the lumbar lordosis angle result in a change in compressive and shear forces, which is reflected in the reconstruction of bone tissue. This can be a clue to differentiate the physiotherapy program of women reporting back pain – depending on the size of the lumbar lordosis angle.
THE CONDITION OF VOLUMETRIC AND OSMOREGULATORY FUNCTION OF THE KIDNEYS IN TOXICEMIA OF PURULENT-SEPTIC GENESIS IN PATIENTS WITH DIABETES MELLITUS
Authors: Konovchuk Viktor M. , Kushnir Sergiy V. , Andrushchak Andriy V. , Iliuk Iryna I. , Moroz Petro V.
Published: October 2020

The aim of the study is to investigate the state of volumetric and osmoregulatory function of the kidneys in diabetes complicated by the syndrome of the endogenous intoxication of purulent-septic genesis.
Materials and methods: The study group was consisted of patients with insulin-dependent diabetes mellitus with complicated syndrome of the endogenous intoxication of purulent-septic genesis (CSDI).
Results: The starting indices of volumetric and osmoregulatory function of the kidneys in patients with INCs complicated by the endogenous intoxication of purulent-septic genesis (CEI GHG) syndrome are characterized by the values of the inhibition of the volumorregulatory (by the clearance of sodium by 11%, p <0.05) and the activation of the osmoregulatory osmotically active substances by 23%, p <0.05) renal function. The volume increasing of the extracellular space by the Ringer solution activates the volumetric and osmoregulatory function of the kidneys, respectively, in patients with NSCL at 162 ± 27.1% (Δ, p <0.05) and 138 ± 48.3% (Δ, p <0.05), and at INCS complicated CEE GHS by 260 ± 47.8% (Δ, p <0.05) and 147 ± 46.9% (Δ, p <0.05).
Conclusions: The low-level isotonic loads with Ringer’s solution initiate the same direction of change in indices of volumetric and osmoregulatory function of the kidneys in patients with systemic inflammatory response syndrome and diabetes mellitus with complicated endogenous intoxication syndrome of purulent-septic genesis and show dissociative hyper reactivity of the volumorregulatory function in relation to the osmoregulatory one.
EARLY PREGNANCY AND ENDOTHELIAL DYSFUNCTION
Authors: Dubyk Lyudmyla V. , Yuzko Oleksandr M. , Chernetska Nataliia V. , Kolotylo Tetiana R.
Published: October 2020

The aim is to analyze the value of endothelial dysfunction markers during pregnancy.
Materials and methods: We have examined 153 pregnant women to identify endothelial dysfunction markers of endothelin-1, nitrogen oxide (NO) that have been studied using immunoenzymometric sets for an uncomplicated and complicated pregnancy.
Results: We found that the concentration of endothelin-1 repeatedly exceeded the rates in pregnant women with miscarriages than during physiological pregnancy. The diametrically opposite pattern concerns the level of nitrogen oxide. These changes in the markers of the functional state of the endothelin indicate the development of the dysfunction of this system in women with the pathology of pregnancy.
Conclusions: Consequently, endothelial dysfunction can be considered to be one of the reasons for miscarriage in the examined women. Therefore, the definition of markers of endothelial dysfunction has prognostic value.
FEATURES OF SOME ANTHROPOMETRIC INDICES AND INDICES OF INSULIN RESISTANCE IN PATIENTS WITH NON-ALCOHOLIC STEATOHEPATITIS ON THE BACKGROUND OF OBESITY AND OSTEOARTHRITIS
Authors: Khukhlina Oksana S. , Liakhovych Oksana D. , Kaniovska Ludmila V. , Kaushanska Olena V. , Antoniv Alona A. , Shuper Vera O.
Published: October 2020

The aim: To estimate the diagnostic and predictive value of anthropometric indices indicating obesity and glycemic parameters in the progression of NASH due to comorbidity with OA and OB.
Materials and methods: 90 patients were examined and distributed into three groups: group 1 included patients suffering from OA, grade II-III according to Kellgren and Lawrense classification, with normal body mass, group 2 -patients with NASH and obesity without OA, group 3 -patients with OA, NASH and obesity. The control group consisted of 30 healthy individuals of the corresponding age.
Results: There was a correlation between lipid and carbohydrate metabolism. This fact was confirmed by the evidence that under the influence of IR there was an increase in the level of lipoproteins enriched in triglycerides, the concentration of cholesterol of low density lipoprotein and reduction in level cholesterol of high density lipoprotein, which led to the progression of the clinical course of NASH. It was established that anthropometric indices of patients with comorbid flow of NASH, OA and OB were significantly (p <0.05) higher than in experimental groups. A positive correlation was found between some anthropometric indices and the following glycemic parameters: fasting blood glucose, HOMA-IR suggested that insulin resistance contributed to the growth of glycated compounds leading not only to the progression of NASH and affect the dysfunction of chondrocytes, but also influencing destruction of subchondral bone in osteoarthritis.
Conclusions: The outcomes of the study result in the necessity of studying the metabolic status in patients with a comorbid progression of NASH, OB and OA for timely correction of the revealed disorders, which will reduce the run of NAFLD.
THE COMPREHENSIVE EVALUATION OF PATIENTS’ CONDITION IN RECOVERY AND RESIDUAL PERIODS OF ANEURYSMAL SUBARACHNOID HEMORRHAGE
Authors: Duve Khrystyna V. , Mishchenko Tamara S. , Shkrobot Svitlana I.
Published: October 2020

The aim: is to evaluate peculiarities of clinical and neurological characteristics, quality of life, brain morphometry changes and metabolic deviations of patients, who suffered from aneurysmal subarachnoid hemorrhage.
Materials and methods: In the period of 2016-2019 we examined 114 patients, who signed the informed consent, taking into account their age, clinical and anatomical form of hemorrhage, disease duration, Hunt-Hess severity grade, complications of acute period. Such parameters were evaluated, as clinical and neurological characteristics, the degree of the Barthel index and the modified Rankin scale, cognitive functioning (MoCA), psycho-emotional sphere and quality of life (HADS, SF-36), morphometric parameters based on brain computed tomography measurements, explored the indicators of apoptosis, mitochondrial dysfunction, intracellular oxidative stress.
Results: Сephalgia (90,35 %), pyramidal syndrome (53,50 %), sensibility deficit (36,84 %) were leading among the all neurological syndromes. Slight dependence and disability grade was found during assessment of the Barthel index and the modified Rankin scale. In 85,96 % of patiens we revealed cognitive impairment of different severity grades. The anxiety was manifested in 65,79 %, depression – in 64,91 % of patients. Due to the morphometry data, the process of cerebral atrophy was detected (central – in 26,31 % of patients, cortical – in 16,67% and mixed – in 28,07 %). AnV+ and PI+ – cells level exceeded normal values in 2,88 and 1,96 times while the level of JC-1+ and ROS+-cells – in 2,17 and 2,82 times (p<0,01).
Conclusions: Having studied clinical and neurological, neuropsychological, morphometric and metabolic factors, we found their pathogenetic role in the course of late recovery and residual periods of aneurysmal subarachnoid hemorrhage, that would help us to improve the diagnostic tactics and reveal the predictors of unfavorable outcome.
DIAGNOSIS OF AUTONOMIC DISORDERS IN PARKINSON’S DISEASE
Authors: Dutkiewicz Justyna , Friedman Andrzej
Published: October 2020

Idiopathic Parkinson’s disease (PD) is a neurodegenerative disorder, characterized by motor and non-motor symptoms. Among non-motor symptoms we distinguish psychotic disorders, memory disorders, autonomic disorders.
The aim: In this article, we want to draw attention to the most common symptoms of dysautonomy in Parkinson’s disease, and the methods of their assessmen and therapy.