bluuebunny/crossref_metadata_2025 huggingface dataset:
Intrathecal Drug Delivery: Advances and Applications in the Management of Chronic Pain Patient
Authors: De Andres Jose , Hayek Salim , Perruchoud Christophe , Lawrence Melinda M. , Reina Miguel Angel , De Andres-Serrano Carmen , Rubio-Haro Ruben , Hunt Mathew , Yaksh Tony L.
Published: June 2022

Advances in our understanding of the biology of spinal systems in organizing and defining the content of exteroceptive information upon which higher centers define the state of the organism and its role in the regulation of somatic and automatic output, defining the motor response of the organism, along with the unique biology and spatial organization of this space, have resulted in an increased focus on therapeutics targeted at this extracranial neuraxial space. Intrathecal (IT) drug delivery systems (IDDS) are well-established as an effective therapeutic approach to patients with chronic non-malignant or malignant pain and as a tool for management of patients with severe spasticity and to deliver therapeutics that address a myriad of spinal pathologies. The risk to benefit ratio of IDD makes it a useful interventional approach. While not without risks, this approach has a significant therapeutic safety margin when employed using drugs with a validated safety profile and by skilled practioners. The present review addresses current advances in our understanding of the biology and dynamics of the intrathecal space, therapeutic platforms, novel therapeutics, delivery technology, issues of safety and rational implementation of its therapy, with a particular emphasis upon the management of pain.
Beneficial Effects of Repeated Washed Microbiota Transplantation in Children With Autism
Authors: Pan Zhao-Yu , Zhong Hao-Jie , Huang Dong-Ni , Wu Li-Hao , He Xing-Xiang
Published: June 2022

Objective

While fecal microbiota transplantation is demonstrated to improve symptoms of autism spectrum disorder (ASD), it remains unclear whether additional treatment courses yield better results. This study sought to evaluate the efficacy of repeated washed microbiota transplantation (WMT) in children with ASD.

Methods

Retrospective data from children who were serially treated with WMT, including ASD symptoms, sleep disorders, gastrointestinal (GI) symptoms, and white blood cell (WBC) and globulin levels were obtained. The effect of WMT on children with ASD and whether additional WMT courses led to a further improvement in symptoms were assessed.

Results

Aberrant Behavior Checklist (ABC), Childhood Autism Rating Scale, and Sleep Disturbance Scale for Children (SDSC) scores, the proportion of children with constipation and abnormal fecal forms, and WBC and globulin levels were all significantly lower in ASD children after WMT. More WMT treatment courses led to significantly lower scores on the ABC and SDSC.

Conclusion

WMT significantly improved ASD and GI symptoms and sleep disorders in children with ASD, and reduced systemic inflammation. Additional WMT courses led to more obvious improvements in ASD symptoms within three treatment courses.
The Effects of a Transgelin-2 Agonist Administered at Different Times in a Mouse Model of Airway Hyperresponsiveness
Authors: Yuan Hong-Kai , Lu Jin , Wang Xue-Ling , Lv Zhi-Ying , Li Bo , Zhu Weiliang , Yang Yong-Qing , Yin Lei-Miao
Published: June 2022

Airway hyperresponsiveness (AHR) is one of the most important features of asthma. Our previous study showed that inhaled transgelin-2 agonist, TSG12, effectively reduced pulmonary resistance in a mouse model of asthma in a dose-dependent manner. However, the optimal administration time of TSG12 to reduce AHR and the pharmacological effects are still unclear. In this study, the effects of TSG12 inhalation before and during AHR occurrence were examined. The results showed that the pulmonary resistance was reduced by 57% and the dynamic compliance was increased by 46% in the TSG12 Mch group (atomize TSG12 10 min before methacholine, p < 0.05 vs. model). The pulmonary resistance was reduced by 61% and the dynamic compliance was increased by 47% in the TSG12 + Mch group (atomize TSG12 and methacholine together, p < 0.05 vs. model). Quantitative real-time PCR showed that the gene expression levels of transgelin-2, myosin phosphatase target subunit-1, and myosin light chain were up-regulated by 6.4-, 1.9-, and 2.8-fold, respectively, in the TSG12 Mch group. The gene expression levels of transgelin-2, myosin phosphatase target subunit-1, and myosin light chain were up-regulated by 3.2-, 1.4-, and 1.9-fold, respectively, in the TSG12 + Mch group. The results suggested that TSG12 effectively reduces pulmonary resistance when TSG12 inhalation occurred both before and during AHR occurrence. Gene expression levels of transgelin-2 and myosin light chain were significantly up-regulated when TSG12 inhalation occurred before AHR occurrence. This study may provide a basis for the administration time of TSG12 for asthma treatment in the future.
Xiaoyaosan Exerts Antidepressant-Like Effect by Regulating Autophagy Involves the Expression of GLUT4 in the Mice Hypothalamic Neurons
Authors: Yang Fu-Rong , Zhu Xiao-Xu , Kong Ming-Wang , Zou Xiao-Juan , Ma Qing-Yu , Li Xiao-Juan , Chen Jia-Xu
Published: June 2022

Many studies have proven that autophagy plays a pivotal role in the development of depression and it also affects the expression of GLUT4 in the hypothalamus. Xiaoyaosan has been shown to exert antidepressant effects in a variety of ways, but its underlying mechanism by which Xiaoyaosan regulates autophagy as well as GLUT4 in the hypothalamus remains unclear. Thus, in this study, we established a mouse model of depression induced by chronic unpredictable mild stress (CUMS), and set up autophagy blockade as a control to explore whether Xiaoyaosan exerts antidepressant effect by affecting autophagy. We examined the effects of Xiaoyaosan on behaviors exhibited during the open field test, tail suspension test and sucrose preference test, and the changes in autophagy in hypothalamic neurons as well as changes in GLUT4 and the related indicators of glucose metabolism in CUMS-induced depressive mouse model. We found that CUMS- and 3-MA-induced mice exhibited depressive-like behavioral changes, with decreased LC3 expression and increased p62 expression, suggesting decreased levels of autophagy in the mouse hypothalamus. The expression of GLUT4 was also decreased, and it was closely related to the level of autophagy through Rab8 and Rab10. Nevertheless, after the intervention of Xiaoyaosan, the above changes were effectively reversed. These results show that Xiaoyaosan can regulate the autophagy in hypothalamic neurons and the expression of GLUT4 in depressed mice.
Chitosan/Sodium Alginate/Velvet Antler Blood Peptides Hydrogel Promoted Wound Healing by Regulating PI3K/AKT/mTOR and SIRT1/NF-κB Pathways
Authors: Hao Mingqian , Peng Xiaojuan , Sun Shuwen , Ding Chuanbo , Liu Wencong
Published: June 2022

Skin wound healing is a principal clinical challenge, and it is necessary to develop effective alternative treatments. Excessive inflammatory response is linked to delayed healing. This study was the first to report a multi-functional chitosan/sodium alginate/velvet antler blood peptides (VBPs) hydrogel (CAVBPH) and explore its potential mechanism to promote wound healing. The results showed that CAVBPH possessed desirable characteristics including thermo-sensitivity, antioxidation, antibacterial activity, biosafety, VBPs release behavior, etc., and significantly accelerated skin wound healing in mice. Specifically, the CAVBPH treatment enhanced cell proliferation, angiogenesis, and extracellular matrix (ECM) secretion, and also relieved inflammation at the wound site compared to the PBS-treated group and blank hydrogel scaffold-treated group. Mechanistically, the efficacy of CAVBPH might be related to the activation of the PI3K/AKT/mTOR and SIRT1/NF-κB pathways. Overall, CAVBPH seems to be a promising therapy for skin repair, probably relying on the abundant short-chain peptides in VBPs.
Population Pharmacokinetics of Colistin Sulfate in Critically Ill Patients: Exposure and Clinical Efficacy
Authors: Yu Xu-ben , Zhang Xiao-Shan , Wang Ye-Xuan , Wang Yu-Zhen , Zhou Hong-Min , Xu Fang-Min , Yu Jun-Hui , Zhang Li-Wen , Dai Ying , Zhou Zi-Ye , Zhang Chun-Hong , Lin Guan-Yang , Pan Jing-Ye
Published: June 2022

Background: Presently, colistin is commercially available in two different forms, namely, colistin sulfate and its sulphomethylated derivative, colistimethate sodium (CMS). However, in the currently reported studies, most of the clinical studies on colistin for parenteral use are referred to as CMS. Data on the pharmacokinetics (PK), clinical efficacy, and side effects of colistin sulfate in clinical use have not been reported.

Methods: This retrospective study was performed on carbapenem-resistant organism (CRO)-infected patients treated with colistin sulfate for more than 72 h. The population pharmacokinetic model was developed using the NONMEM program. The clinical outcomes including clinical treatment efficacy, microbiological eradication, and nephrotoxicity were assessed. Monte Carlo simulation was utilized to calculate the probability of target attainment (PTA) in patients with normal or decreased renal function.

Results: A total of 42 patients were enrolled, of which 25 (59.52%) patients were considered clinical treatment success and 29 (69.06%) patients had successful bacteria elimination at the end of treatment. Remarkably, no patient developed colistin sulfate-related nephrotoxicity. A total of 112 colistin concentrations with a range of 0.28–6.20 mg/L were included for PK modeling. The PK characteristic of colistin was well illustrated by a one-compartment model with linear elimination, and creatinine clearance (CrCL) was identified as a covariate on the clearance of colistin sulfate that significantly explained inter-individual variability. Monte Carlo simulations showed that the recommended dose regimen of colistin sulfate, according to the label sheet, of a daily dose of 1–1.5 million IU/day, given in 2–3 doses, could attain PTA > 90% for MICs ≤ 0.5 μg/mL, and that a daily dose of 1 million IU/day could pose a risk of subtherapeutic exposure for MIC ≥1 μg/ml in renal healthy patients.

Conclusion: Renal function significantly affects the clearance of colistin sulfate. A dose of 750,000 U every 12 h was recommended for pathogens with MIC ≤1 μg/ml. The dosage recommended by the label inserts had a risk of subtherapeutic exposure for pathogens with MIC ≥2 μg/ml. Despite higher exposure to colistin in patients with acute renal insufficiency, dose reduction was not recommended.
Santalol Isomers Inhibit Transthyretin Amyloidogenesis and Associated Pathologies in Caenorhabditis elegans
Authors: Mohankumar Amirthalingam , Kalaiselvi Duraisamy , Thiruppathi Govindhan , Muthusaravanan Sivaramakrishnan , Vijayakumar Subramaniam , Suresh Rahul , Tawata Shinkichi , Sundararaj Palanisamy
Published: June 2022

Transthyretin (TTR) is a homotetrameric protein found in human serum and is implicated in fatal inherited amyloidoses. Destabilization of native TTR confirmation resulting from mutation, environmental changes, and aging causes polymerization and amyloid fibril formation. Although several small molecules have been reported to stabilize the native state and inhibit TTR aggregation, prolonged use can cause serious side effects. Therefore, pharmacologically enhancing the degradation of TTR aggregates and kinetically stabilizing the native tetrameric structure with bioactive molecule(s) could be a viable therapeutic strategy to hinder the advancement of TTR amyloidoses. In this context, here we demonstrated α- and β-santalol, natural sesquiterpenes from sandalwood, as a potent TTR aggregation inhibitor and native state stabilizer using combined in vitro, in silico, and in vivo experiments. We found that α- and β-santalol synergize to reduce wild-type (WT) and Val30Met (V30M) mutant TTR aggregates in novel C. elegans strains expressing TTR fragments fused with a green fluorescent protein in body wall muscle cells. α- and β-Santalol extend the lifespan and healthspan of C. elegans strains carrying TTRWT::EGFP and TTRV30M::EGFP transgene by activating the SKN-1/Nrf2, autophagy, and proteasome. Moreover, α- and β-santalol directly interacted with TTR and reduced the flexibility of the thyroxine-binding cavity and homotetramer interface, which in turn increases stability and prevents the dissociation of the TTR tetramer. These data indicate that α- and β-santalol are the strong natural therapeutic intervention against TTR-associated amyloid diseases.
Fisetin Attenuated Oxidative Stress-Induced Cellular Damage in ARPE-19 Human Retinal Pigment Epithelial Cells Through Nrf2-Mediated Activation of Heme Oxygenase-1
Authors: Park Cheol , Noh Jeong Sook , Jung Youngmi , Leem Sun-Hee , Hyun Jin Won , Chang Young-Chae , Kwon Taeg Kyu , Kim Gi-Young , Lee Hyesook , Choi Yung Hyun
Published: June 2022

Fisetin is a kind of bioactive flavonol, widely present in various fruits such as strawberries and apples, and is known to act as a potent free radical scavenger. However, the mechanism of action related to the antioxidant activity of this compound in human retinal pigment epithelial (RPE) cells is not precisely known. In this study, we aimed to investigate whether fisetin could attenuate oxidative stress-induced cytotoxicity on human RPE ARPE-19 cells. To mimic oxidative stress, ARPE-19 cells were treated with hydrogen peroxide (H2O2), and fisetin significantly inhibited H2O2-induced loss of cell viability and increase of intracellular reactive oxygen species (ROS) production. Fisetin also markedly attenuated DNA damage and apoptosis in H2O2-treated ARPE-19 cells. Moreover, mitochondrial dysfunction in H2O2-treated cells was alleviated in the presence of fisetin as indicated by preservation of mitochondrial membrane potential, increase of Bcl-2/Bax expression ratio, and suppression of cytochrome c release into the cytoplasm. In addition, fisetin enhanced phosphorylation and nuclear translocation of nuclear factor erythroid 2 related factor 2 (Nrf2), which was associated with increased expression and activity of heme oxygenase-1 (HO-1). However, the HO-1 inhibitor, zinc protoporphyrin, significantly reversed the protective effect of fisetin against H2O2-mediated ARPE-19 cell injury. Therefore, our results suggest that Nrf2-mediated activation of antioxidant enzyme HO-1 may play an important role in the ROS scavenging activity of fisetin in RPE cells, contributing to the amelioration of oxidative stress-induced ocular disorders.
Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities
Authors: Zhao Lei , Wang Lei , Guo Zimeng , Zhang Ning , Feng Qisheng , Li Bo
Published: June 2022

Two polysaccharide fractions from Pogostemon cablin (Blanco) Benth. (P. cablin) (designated as PCB-1 and PCB2-1) were isolated by water extraction and purified by Sepharose chromatography. The chemical properties of the polysaccharides were characterised, and their antioxidant activities were evaluated. The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively. The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively. Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios. The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities. In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver. These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
Aberration Compensated Point Light Source Display With High-Resolution
Authors: Cao Yulian , Chagnaadorj Byamba-Ochir , Dalkhaa Nomin-Erdene , Baasantseren Ganbat
Published: June 2022

A high-resolution enhanced point light source integral imaging display is proposed. Using additional light sources to create extra point light sources in the point light source plane, the point light sources appeared on the plane that deviated from the expected values because of aberrations in the lens. Previously, only the lens array aberration was corrected and the distance along the horizontal and vertical axes was corrected too. The objective of this paper is to simultaneously correct the aberration between the lens array and the collimating lens, plus the horizontal and vertical axes, and correct the error along the z-axis. So, we determined the distance between the central light source and the additional light source to compensate for lens aberrations. From the experimental results, our technique precisely increased the resolution 3 times in both vertical and horizontal dimensions when compared to a traditional point light source display. Our method is applicable to 3D displays and compensates for the lens array and collimating lens aberrations.
Timelike-Ruled and Developable Surfaces in Minkowski 3-Space E13
Authors: Mofarreh Fatemah
Published: June 2022

In this study, the timelike-ruled and developable surfaces are constructed in Minkowski 3-spaceE13. Using the E. Study map, we demonstrate that dual forms of timelike-ruled and developable surfaces can be obtained from the coordinates and first derivatives of the base curve at the dual hyperbolic unit sphere. This is proposed as a novel method for obtaining timelike-ruled and developable surfaces. Some examples have also been provided.
Mechanosensitive Hydrolysis of ATP and ADP in Lamina Propria of the Murine Bladder by Membrane-Bound and Soluble Nucleotidases
Authors: Aresta Branco Mafalda S. L. , Gutierrez Cruz Alejandro , Dayton Jacob , Perrino Brian A. , Mutafova-Yambolieva Violeta N.
Published: June 2022

Prior studies suggest that urothelium-released adenosine 5′-triphosphate (ATP) has a prominent role in bladder mechanotransduction. Urothelial ATP regulates the micturition cycle through activation of purinergic receptors that are expressed in many cell types in the lamina propria (LP), including afferent neurons, and might also be important for direct mechanosensitive signaling between urothelium and detrusor. The excitatory action of ATP is terminated by enzymatic hydrolysis, which subsequently produces bioactive metabolites. We examined possible mechanosensitive mechanisms of ATP hydrolysis in the LP by determining the degradation of 1,N6-etheno-ATP (eATP) at the anti-luminal side of nondistended (empty) or distended (full) murine (C57BL/6J) detrusor-free bladder model, using HPLC. The hydrolysis of eATP and eADP was greater in contact with LP of distended than of nondistended bladders whereas the hydrolysis of eAMP remained unchanged during filling, suggesting that some steps of eATP hydrolysis in the LP are mechanosensitive. eATP and eADP were also catabolized in extraluminal solutions (ELS) that were in contact with the LP of detrusor-free bladders, but removed from the organ chambers prior to addition of substrate. The degradation of both purines was greater in ELS from distended than from nondistended preparations, suggesting the presence of mechanosensitive release of soluble nucleotidases in the LP. The released enzyme activities were affected differently by Ca2+and Mg2+. The common nucleotidase inhibitors ARL67156, POM-1, PSB06126, and ENPP1 Inhibitor C, but not the alkaline phosphatase inhibitor (-)-p-bromotetramisole oxalate, inhibited the enzymes released during bladder distention. Membrane-bound nucleotidases were identified in tissue homogenates and in concentrated ELS from distended preparations by Wes immunodetection. The relative distribution of nucleotidases was ENTPD1 >> ENPP1 > ENTPD2 = ENTPD3 > ENPP3 = NT5E >> ENTPD8 = TNAP in urothelium and ENTPD1 >> ENTPD3 >> ENPP3 > ENPP1 = ENTPD2 = NT5E >> ENTPD8 = TNAP in concentrated ELS, suggesting that regulated ectodomain shedding of membrane-bound nucleotidases possibly occurs in the LP during bladder filling. Mechanosensitive degradation of ATP and ADP by membrane-bound and soluble nucleotidases in the LP diminishes the availability of excitatory purines in the LP at the end of bladder filling. This might be a safeguard mechanism to prevent over-excitability of the bladder. Proper proportions of excitatory and inhibitory purines in the bladder wall are determined by distention-associated purine release and purine metabolism.
Population Structure and Spatial Distribution Pattern of Populus euphratica Riparian Forest Under Environmental Heterogeneity Along the Tarim River, Northwest China
Authors: Yusup Asadilla , Halik Ümüt , Abliz Abdulla , Aishan Tayierjiang , Keyimu Maierdang , Wei Jianxin
Published: June 2022

Populus euphratica Oliv. (Euphrates poplar), as the dominant tree species of desert riparian forests along the Central Asian inland rivers, plays a critical role in protecting arid land ecosystems. In recent decades, climate change and excessive water resources utilization activities have led to the environmental degradation of desert riparian forests along the Tarim River in northwest China. Understanding the forest stand structure and spatial distribution pattern provide important guidance for monitoring forest dynamics in support of sustainable management. However, few studies have examined how riparian forests stand attributes differ in response to environmental heterogeneity. In this study, terrestrial laser scanning (TLS) was applied to acquire a total of 1648 individual P. euphratica tree’s 3D structure attributes within 18 plots along the upper, middle, and lower reaches of the Tarim River, which included tree height (TH), diameter at breast height (DBH), crown diameter (CD), crown projection area (CPA), stand density index (SDI), age structure ratios, and spatial pattern. The results showed that the average tree segmentation and structure determination accuracies of TLS were 93.2 and 94.6%. From the upper to the lower reaches, the average TH and CD decreased by 3.8 and 0.3 m, while the DBH increased by 4.2 cm. The SDI and CPA exhibited the following order: upper reaches (454 n ha–1, 82.3%) > middle reaches (382 n ha–1, 67.3%) > lower reaches (263 n ha–1, 39.1%), the differences were significant at 0.05 level. The population age structure changed from growing population in the upper reaches to stable population in the middle and a temporarily stable population in the lower reaches. The pair correlation g(r) function determined random distribution pattern in the upper reaches [g(r) = 1.2], an aggregated pattern in the middle [g(r) = 3.1], and lower reaches [g(r) = 9.7]. The decline in groundwater depth and soil moisture increased aggregated distribution pattern (R = 0.67 and 0.56, P < 0.05) of the P. euphratica along the mainstream of Tarim River. The results enrich our understanding of the current development stage of P. euphratica, which is important for optimizing management strategies and realizing the sustainability of floodplain ecosystems.
Evaluation of the Biostimulant Activity of Zaxinone Mimics (MiZax) in Crop Plants
Authors: Wang Jian You , Jamil Muhammad , Hossain Md. Golap , Chen Guan-Ting Erica , Berqdar Lamis , Ota Tsuyoshi , Blilou Ikram , Asami Tadao , Al-Solimani Samir Jamil , Mousa Magdi Ali Ahmed , Al-Babili Salim
Published: June 2022

Global food security is a critical concern that needs practical solutions to feed the expanding human population. A promising approach is the employment of biostimulants to increase crop production. Biostimulants include compounds that boost plant growth. Recently, mimics of zaxinone (MiZax) were shown to have a promising growth-promoting effect in rice (Oryza sativa). In this study, we investigated the effect of MiZax on the growth and yield of three dicot horticultural plants, namely, tomato (Solanum lycopersicum), capsicum (Capsicum annuum), and squash (Cucurbita pepo) in different growth environments, as well as on the growth and development of the monocot date palm (Phoenix dactylifera), an important crop in the Middle East. The application of MiZax significantly enhanced plant height, flower, and branch numbers, fruit size, and total fruit yield in independent field trials from 2020 to 2021. Importantly, the amount of applied MiZax was far less than that used with the commercial compound humic acid, a widely used biostimulant in horticulture. Our results indicate that MiZax have significant application potential to improve the performance and productivity of horticultural crops.
Identification of Candidate Genes and Genomic Selection for Seed Protein in Soybean Breeding Pipeline
Authors: Qin Jun , Wang Fengmin , Zhao Qingsong , Shi Ainong , Zhao Tiantian , Song Qijian , Ravelombola Waltram , An Hongzhou , Yan Long , Yang Chunyan , Zhang Mengchen
Published: June 2022

Soybean is a primary meal protein for human consumption, poultry, and livestock feed. In this study, quantitative trait locus (QTL) controlling protein content was explored via genome-wide association studies (GWAS) and linkage mapping approaches based on 284 soybean accessions and 180 recombinant inbred lines (RILs), respectively, which were evaluated for protein content for 4 years. A total of 22 single nucleotide polymorphisms (SNPs) associated with protein content were detected using mixed linear model (MLM) and general linear model (GLM) methods in Tassel and 5 QTLs using Bayesian interval mapping (IM), single-trait multiple interval mapping (SMIM), single-trait composite interval mapping maximum likelihood estimation (SMLE), and single marker regression (SMR) models in Q-Gene and IciMapping. Major QTLs were detected on chromosomes 6 and 20 in both populations. The new QTL genomic region on chromosome 6 (Chr6_18844283–19315351) included 7 candidate genes and the Hap.XAA at the Chr6_19172961 position was associated with high protein content. Genomic selection (GS) of protein content was performed using Bayesian Lasso (BL) and ridge regression best linear unbiased prediction (rrBULP) based on all the SNPs and the SNPs significantly associated with protein content resulted from GWAS. The results showed that BL and rrBLUP performed similarly; GS accuracy was dependent on the SNP set and training population size. GS efficiency was higher for the SNPs derived from GWAS than random SNPs and reached a plateau when the number of markers was >2,000. The SNP markers identified in this study and other information were essential in establishing an efficient marker-assisted selection (MAS) and GS pipelines for improving soybean protein content.
An Antifungal Role of Hydrogen Sulfide on Botryosphaeria Dothidea and Amino Acid Metabolism Involved in Disease Resistance Induced in Postharvest Kiwifruit
Authors: Duan Bing , Du Huaying , Zhang Wei , Wang Jing , Cai Zhipeng , Shen Yonggen , Huang Tenghuan , Yuan Jie , Gan Zengyu , Chen Jinyin , Zhu Liqin
Published: June 2022

Botryosphaeria dothidea is a major pathogen responsible for postharvest kiwifruit soft rot. This study aimed to determine the influence of hydrogen sulfide (H2S) on postharvest resistance to kiwifruit soft rot and the antifungal role of H2S against B. dothidea. The results indicated that H2S (20 μl L−1) restricted the lesion area following inoculation with B. dothidea. H2S enhanced the production of shikimic acid, tyrosine, tryptophan, and phenylalanine while also increasing the total phenols, flavonoids, and lignin. H2S upregulated the expression of AcDHQS, AcSDH, AcSK, AcPAL, AcCAD, and AcCHS. Additionally, sodium hydrosulfide (NaHS)-released H2S inhibited mycelial growth. NaHS concentrations of 20 and 40 mmol L−1 significantly decreased the mycelial weight and malondialdehyde content (MDA) content while increasing cell membrane conductivity and membrane leakage. The results indicate that H2S induces resistance in kiwifruit via a microbicidal role and amino acid metabolism involved in postharvest kiwifruit disease resistance.
Application of UAV Remote Sensing in Monitoring Water Use Efficiency and Biomass of Cotton Plants Adjacent to Shelterbelt
Authors: Ma Bin , Wang Qijie , Xue Bing , Hou Zhenan , Jiang Yan , Cai Wenyue
Published: June 2022

Tree shelterbelts are crucial for maintaining the ecological environment of oasis, but they may also compete for soil water with adjacent crops, affecting crop yields. To evaluate the impacts of the shelterbelt on water use efficiency (WUE) and normalized water productivity (WP) of adjacent cotton plants, the biomass (B) and WUE of cotton with different distances from the shelterbelt (0.1H, 0.5H, 1H, 2H, and 3H; average tree height = 15 m [H]) were estimated based on unmanned aerial vehicle (UAV) remote sensing data combined with the FAO crop water response model AquaCrop. Besides, the accuracy and universality of the estimation method were also evaluated. The results showed that the method based on UAV remote sensing data and AquaCrop can accurately estimate the impact range and intensity of shelterbelt on WUE, water consumption, and B of adjacent cotton plants. Fierce water competition between shelterbelt and cotton was detected within 0.1H−1H, and the competitiveness of the shelterbelt was weaker in the plots >1H than in the 0.1H−1H. The B, actual evapotranspiration (Tc), and WUE of cotton at 0.1H decreased by 59.3, 48.8, and 23.6%, respectively, compared with those at 3H, but the cotton plants at 2H and 3H were completely unaffected by the shelterbelt. Besides, the B estimated based on WP (root mean square error [RMSE] = 108 g/m2, d = 0.89) was more accurate than that estimated based on WUE (RMSE = 118 g/m2, d = 0.85). This study clarifies the inter-species competition for soil water between crops and shelterbelts under drip irrigation in oases in China.
Comprehensive Effects of Flowering Locus T-Mediated Stem Growth in Tobacco
Authors: Wu Jun , Wu Qiuhong , Bo Zhongjian , Zhu Xuli , Zhang Junhui , Li Qingying , Kong Wenqing
Published: June 2022

In flowering plants, Flowering locus T (FT) encodes a major florigen. It is a key flowering hormone in controlling flowering time and has a wide range of effects on plant development. Although the mechanism by which FT promotes flowering is currently clearly understood, comprehensive effects of the FT gene on plant growth have not been evaluated. Therefore, the effects of FT on vegetative growth need to be explored for a complete understanding of the molecular functions of the FT gene. In this study, the Jatropha curcas L. FT gene was overexpressed in tobacco (JcFTOE) in order to discover multiple aspects and related mechanisms of how the FT gene affects plant development. In JcFTOE plants, root, stem, and leaf development was strongly affected. Stem tissues were selected for further transcriptome analysis. In JcFTOE plants, stem growth was affected because of changes in the nucleus, cytoplasm, and cell wall. In the nucleus of JcFTOE plants, the primary effect was to weaken all aspects of DNA replication, which ultimately affected the cell cycle and cell division. The number of stem cells decreased significantly in JcFTOE plants, which decreased the thickness and height of tobacco stems. In the cell wall of JcFTOE plants, hemicellulose and cellulose contents increased, with the increase in hemicellulose associated with up-regulation of xylan synthase-related genes expression. In the cytoplasm of JcFTOE plants, the primary effects were on biogenesis of ribonucleoprotein complexes, photosynthesis, carbohydrate biosynthesis, and the cytoskeleton. In addition, in the cytoplasm of JcFTOE plants, there were changes in certain factors of the core oscillator, expression of many light-harvesting chlorophyll a/b binding proteins was down-regulated, and expression of fructose 1,6-bisphosphatase genes was up-regulated to increase starch content in tobacco stems. Changes in the xylem and phloem of JcFTOE plants were also identified, and in particular, xylem development was affected by significant increases in expression of irregular xylem genes.
Optimizing Wheat Yield, Water, and Nitrogen Use Efficiency With Water and Nitrogen Inputs in China: A Synthesis and Life Cycle Assessment
Authors: Li Zhou , Cui Song , Zhang Qingping , Xu Gang , Feng Qisheng , Chen Chao , Li Yuan
Published: June 2022

To meet the demand of the fast increasing population, enhancing the wheat (Triticum aestivum L.) yield and resource use efficiency by optimizing water and nitrogen (N) management can greatly improve agricultural sustainability and enhance regenerative farming in developing countries such as China. Based on 126 studies conducted in China between 1996 and 2018, using meta-analysis in combination with decision regression tree modeling and life cycle assessment (LCA), this study aimed to (1) quantify the effect of water and N input on wheat yield, water productivity (WPc), and N use efficiency (NUEf), and evaluate the subsequent environmental impact in different regions using LCA; and (2) evaluate, model, and rank the roles of environmental (e.g., soil nutrient status and climatic factors) and agronomic factors (e.g., water and N management practices) affecting wheat yield, WPc, and NUEf. The results showed that irrigation and N addition increased the average yield and WPc by 40 and 15%, respectively, relative to control treatments with no irrigation or fertilizer application. The mean water saving potential (WSP) and N saving potential (NSP) in China were estimated at 11 and 10%, respectively. Soil nutrient status [e.g., initial soil phosphorus (P) and potassium (K)] and soil organic carbon content affected the wheat yield, WPc, and NUEf more significantly than climatic factors [mean annual temperature (MAT)] or water and N management practices. The structural equation-based modeling indicated that initial soil nutrient condition impacted productivity and resource use efficiency more at the below optimal water and N levels than above. The risk-factor-based feature ranking indicated that site-specific environmental and soil condition was highly informative toward model construction but split input of N or water had less impact on yield and input use efficiency. LCA demonstrated that to further mitigate greenhouse gas emissions, water- or N-saving management should be promoted in China. Collectively, our research implies that long-term soil health and nutrient enhancement should be more beneficial for increasing yield and resource use efficiency in wheat production.
Transcriptome Analysis Revealed the Molecular Response Mechanism of High-Resistant and Low-Resistant Alfalfa Varieties to Verticillium Wilt
Authors: Li Fang , Chen Xi , Yang Bo , Guang Yingjie , Wu Dandan , Shi Zunji , Li Yanzhong
Published: June 2022

Following infestation by Verticillium wilt, alfalfa (Medicago sativa L.) often shows symptoms such as disease spots, leaf loss, stem, and leaf yellowing, resulting in the decline of alfalfa yield and quality and causing significant losses to the alfalfa industry. The popularization and planting of disease-resistant varieties is the most effective method to prevent and control Verticillium wilt of alfalfa. Therefore, it is particularly important to reveal the resistance mechanism of Verticillium wilt resistant varieties of alfalfa. In this study, the physiological and biochemical indexes were measured on days 7, 14, 21, and 28 after inoculation with Verticillium alfalfae for investigating the response mechanisms of two alfalfa varieties, high-resistant WL343HQ, and low-resistant Dryland. Transcriptome sequencing of alfalfa samples infected with V. alfalfae and uninfected alfalfa samples was performed to analyze the potential functions and signaling pathways of differentially expressed genes (DEGs) by GO classification and KEGG enrichment analysis. Meanwhile, weighted gene co-correlation network analysis (WGCNA) algorithm was used to construct a co-expression network of DEGs. Inoculation with V. alfalfae significantly affected net photosynthetic rate, stomatal conductance, chlorophyll content, MDA content, JA and SA concentrations, and NO and H2O2 contents in both WL343HQ and Dryland inoculated with V. alfalfae. Most of the transcription factors in plants were classified in the WRKY, NAC, and bHLH families. WGCNA analysis showed that the number of transcription factors related to plant growth and disease resistance was higher in the corresponding modules of WL343HQ disease groups on days 7 and 28 (WVa) and (WVd) than in the corresponding modules of Dryland disease groups on days 7 and 21 (HVa) and (HVc). These findings provide data for further gene function validation and also provide a reference for in-depth studies on interactions between plants and pathogens.
Workplace Inclusion of People With Health Issues, Immigrants, and Unemployed Youths—A Qualitative Study of Norwegian Leaders’ Experiences
Authors: Johnsen Tone Langjordet , Fyhn Tonje , Jordbru Anika , Torp Steffen , Tveito Torill Helene , Øyeflaten Irene
Published: June 2022

Aim

To explore leaders’ perceptions and experiences of facilitators and barriers for successful workplace inclusion of immigrants, unemployed youths, and people who are outside the labor market due to health issues.

Methods

Semi-structured individual interviews with 16 leaders who actively engaged in inclusion work, representing different occupations, were conducted. Systematic Text Condensation was used to structure the analysis.

Results

The participating leaders emphasized that job match, including their perception of workers’ motivation, respect for workplace policies, and the availability of appropriate accommodation at the workplace, facilitated work inclusion. An active public support system providing professional and financial support to workers and leaders was also an important facilitating factor. The leaders emphasized that their perception of workers’ lack of motivation for the job was the most important barrier in their own hiring and inclusion engagement. Successful inclusion depended on all workers acknowledging responsibility for and contributing to an inclusive work environment. Being open and willing to discuss challenges was an important part of making the inclusion work. In addition, leadership qualities, such as empathy, patience, and a non-judgmental attitude, appeared as a hallmark among these leaders who actively engaged in inclusion work.

Conclusion

Workplace inclusion of this population of marginalized people was facilitated by job match, mutual respect, commitment, and trust, as well as financial and practical support from the public support system. Leaders’ inclusion practices were furthermore affected by personal attitudes and perceptions of social responsibility. Even so, successful workplace inclusion was presented as a two-way street. Leaders have the main responsibility in initiating a respectful and trusting relationship, but both the worker and the leader needs to contribute to make the relationship thrive.
Self-Prioritization Effect in Children and Adults
Authors: Singh Divita , Karnick Harish
Published: June 2022

Self-related information is processed with priority, an effect known as the self-prioritization effect (SPE). Recent studies on SPE show enhanced cognitive processing of the newly learned self-association compared to non-self (such as mother, friend, and stranger) associations among younger and older adults. However, developmental influences on the magnitude of SPE remain poorly understood. In order to examine the developmental impacts on the SPE, in the present study, we recruited participants ranging from 9–22 years of age and divided them into three age groups: older children (age 9–13), teenagers (age 14–17), and young adult (age 18–22) and compared their performance in the matching judgment task. Our results show more significant bias toward self than mother, friend, or stranger condition in all the three age groups, showing robust SPE in the 9-22-year-old age group. We also observed a more significant bias toward mother-association than friend and stranger-association in all the age groups showing an enhanced bias toward mother. Our study extends the SPE in older children and teenagers and shows that SPE remains robust and stable throughout childhood.
Treatment Satisfaction With Couplelinks Online Intervention to Promote Dyadic Coping in Young Couples Affected by Breast Cancer
Authors: Fergus Karen , Tanen Adina , Ahmad Saunia , Gardner Sandra , Warner Ellen , McLeod Deborah , Stephen Joanne , Carter Wendy , Periera Amanda
Published: June 2022

Background

This study evaluated participant satisfaction with “Couplelinks,” an online psychological intervention designed for younger couples coping with breast cancer. The program included six experiential learning exercises (plus one optional module), psychoeducational information, and support from a personal mental health professional.

Objective

The primary objectives were to examine participants’ perceptions of: the online intervention’s structure and content; the value of including a professional facilitator; and benefits and drawbacks of the program.

Methods

A treatment satisfaction questionnaire comprised of Likert indices and open-ended questions pertaining to treatment satisfaction was completed by 26 patients and 27 male partners (N = 53) approximately 1–2 weeks following the intervention which occurred in the context of a randomized controlled trial. Descriptive statistics were used to summarize satisfaction ratings and generalized linear models with fixed effect for gender were used to test for differences in male-female outcomes. A thematic analysis was undertaken in order to understand, organize and summarize the qualitative textual feedback.

Results

Participants reported an overall satisfaction rating of 4.3 out of 5 (SD = 0.54) with patient satisfaction ratings being higher than that of male partners’ (p = 0.01). The majority of participants considered the facilitator’s role to be necessary 4.6 (SD = 0.60), and found the program to be convenient 4.1 (SD = 0.81) despite some participants struggling to keep up with the modules. Subjective data revealed participants valued the convenience and flexibility of the online intervention and appreciated the program’s involvement of both partners. Participants also reported that including a professional facilitator humanized the intervention, served as motivation to progress through the program, facilitated insight into their relationship, and was reassuring. Experiential gains noted by participants included that the program: helped couples to open channels of communication; prompted them to designate quality time for one another; evoked feelings of unity and togetherness; and inspired new insight in the relationship.

Conclusion

Such feedback supports the feasibility and acceptability of the Couplelinks program while offering directions for improvement of online couple-based interventions in cancer.
How Do Employees Understand Negative Leadership? The Non-linear Relationship Between Abusive Supervision and Employee Innovation Behavior: Job Performance as a Moderator
Authors: Yuan Kuo-Shu , Ng Tung , Wu Tung-Ju
Published: June 2022

This study aims to investigate the non-linear relationship between abusive supervision and employee innovation behavior and further examine how job performance moderates this relationship. Two hundred and seventy-six employees across three industries (restaurant service, tourism, and financial service) in China participated in this study and completed the survey at two time points. The results of curve regression show as follows: (1) There exists a non-linear relationship between abusive supervision and innovation behavior, and (2) job performance moderates the non-linear relationship between abusive supervision and employee innovation behavior. For employees who perform well at work, there exists a U-shaped relationship between abusive supervision and innovative behavior. Whereas, for employees with poor job performance, when abusive supervision reaches a certain level, it will promote employee innovation behavior; and the excess of abusive supervision will inhibit employee innovation behavior, showing an inverted U-shaped curve relationship. The finding suggests it is important for managers to understand the stakes arising from abusive supervision. That is, managers should manipulate the right level of abuse supervision to promote employee innovation behavior.
The Impact of Emotional States on Construction Workers’ Recognition Ability of Safety Hazards Based on Social Cognitive Neuroscience
Authors: Chong Dan , Yu Anni , Su Hao , Zhou Yue
Published: June 2022

The construction industry is one of the most dangerous industries with grave situation owing to high accident rate and mortality rate, which accompanied with a series of security management issues that need to be tackled urgently. The unsafe behavior of construction workers is a critical reason for the high incidence of safety accidents. Affective Events Theory suggests that individual emotional states interfere with individual decisions and behaviors, which means the individual emotional states can significantly influence construction workers’ unsafe behaviors. As the complexity of the construction site environment and the lack of attention to construction workers’ emotions by managers, serious potential emotional problems were planted, resulting in the inability of construction workers to effectively recognize safety hazards, thus leading to safety accidents. Consequently, the study designs a behavioral experiment with E-prime software based on social cognitive neuroscience theories. Forty construction workers’ galvanic skin response signals were collected by a wearable device (HKR-11C+), and the galvanic skin response data were classified into different emotional states with support vector machine (SVM) algorithm. Variance analysis, correlation analysis and regression analysis were used to analyze the influence of emotional states on construction workers’ recognition ability of safety hazards. The research findings indicate that the SVM algorithm could effectively classify galvanic skin response data. The construct ion workers’ the reaction time to safety hazards and emotional valence were negatively correlated, while the accuracy of safety hazards recognition and the perception level of safety hazard separately had an inverted “U” type relationship with emotional valence. For construction workers with more than 20 years of working experience, work experience could effectively reduce the influence of emotional fluctuations on the accuracy of safety hazards identification. This study contributes to the application of physiological measurement techniques in construction safety management and shed a light on improving the theoretical system of safety management.