bluuebunny/crossref_metadata_2025 huggingface dataset:
Hematological and Hematopoietic Effects of Bactericidal Doses of Trans-Cinnamaldehyde and Thyme Oil on Cyprinus carpio Juveniles
Authors: Kondera Elżbieta , Bojarski Bartosz , Ługowska Katarzyna , Kot Barbara , Witeska Małgorzata
Published: November 2021

The effects of two potential antibacterial agents of plant origin: trans-cinnamaldehyde (TC) and thyme oil (TO) on the peripheral blood parameters and cellular composition of hematopoietic tissue of Cyprinus carpio were studied. Both phytochemicals were used in the doses based on the bactericidal concentrations against Aeromonas spp. developed earlier in in vitro study. The fish were fed for 2 weeks on a commercial feed supplemented with 10 μl/kg of TC or 20 μl/kg of TO. Groups TC1 and TO1 were fed diets containing phytochemicals daily, while groups TC2 and TO2 every 2 days. Control group and groups TC2 and TO2 on the remaining days were fed plain feed. Peripheral blood and head kidney hematopoietic tissue were sampled from all the fish at the end of the experiment. In all the groups, hematological values were within the reference ranges for the healthy common carp juveniles. However, blood hemoglobin (Hb) concentration and mean corpuscular hemoglobin concentration (MCHC) were significantly lower in all the groups exposed to TC and TO, while MCH in TC1, TO1, and TO2 compared to the control. TC and TO did not affect leukocyte count [white blood cell (WBC)], differential leukocyte count, the oxidative activity of phagocytes [nitroblue tetrazolium (NBT)], or thrombocyte count (Thro). No significant alterations were observed in the hematopoietic tissue. The results showed that TC and TO exhibited no considerable hematotoxic effects and trials of their use in the treatment of fish infected with Aeromonas spp. may be undertaken.
The Anaphase Promoting Complex/Cyclosome Subunit 11 and Its Role in Organ Size and Plant Development
Authors: Schwedersky Rodrigo Porto , Saleme Marina de Lyra Soriano , Rocha Ingrid Andrade , Montessoro Patricia da Fonseca , Hemerly Adriana Silva , Eloy Nubia Barbosa , Ferreira Paulo Cavalcanti Gomes
Published: November 2021

The anaphase promoting complex/cyclosome (APC/C), a member of the E3 ubiquitin ligase family, plays an important role in recognizing the substrates to be ubiquitylated. Progression of anaphase, and therefore, of the cell cycle, is coordinated through cyclin degradation cycles dependent on proteolysis triggered by APC/C. The APC/C activity depends on the formation of a pocket comprising the catalytic subunits, APC2, APC11, and APC10. Among these, the role of APC11 outside the cell division cycle is poorly understood. Therefore, the goal of this work was to analyze the function of APC11 during plant development by characterizing apc11 knock-down mutant lines. Accordingly, we observed decreased apc11 expression in the mutant lines, followed by a reduction in meristem root size based on the cortical cell length, and an overall size diminishment throughout the development. Additionally, crosses of apc11-1 and amiR-apc11 with plants carrying a WUSCHEL-RELATED HOMEOBOX5 (WOX5) fluorescent marker showed a weakening of the green fluorescent protein-positive cells in the Quiescent Center. Moreover, plants with apc11-1 show a decreased leaf area, together with a decrease in the cell area when the shoot development was observed by kinematics analysis. Finally, we observed a decreased APC/C activity in the root and shoot meristems in crosses of pCYCB1;1:D-box-GUS with apc11-1 plants. Our results indicate that APC11 is important in the early stages of development, mediating meristematic architecture through APC/C activity affecting the overall plant growth.
Root Metabolite Differences in Two Maize Varieties Under Lead (Pb) Stress
Authors: Zhang Hanqian , Qin Yuying , Huang Kai , Zhan Fangdong , Li Ru , Chen Jianjun
Published: November 2021

To assess root metabolic differences of maize varieties in their response to lead (Pb) stress, the lead-tolerant variety Huidan No. 4 and the lead-sensitive variety Ludan No. 8 were tested under Pb-free and Pb-stressed conditions. Changes in metabolites were measured using ultra-performance liquid chromatography–mass spectrometry. Pb stress changed the levels of the amino acids proline, glutamine, lysine, and arginine in both varieties, whereas glutamate and phenylalanine levels changed only in Huidan No. 4. Pb stress altered cystine, valine, methionine, and tryptophan levels only in Ludan No. 8. Therefore, the synthesis and decomposition of amino acids may affect the response of maize to Pb stress. The degree of change in differential metabolites for Huidan No. 4 was greater than that for Ludan No. 8. In cell wall subcellular components, increases in superoxide dismutase (SOD), peroxidases (PODs), and Pb concentrations were greater in Huidan No. 4 than in Ludan No. 8. Therefore, the greater Pb tolerance of Huidan No. 4 could be due to better sequestration of Pb in cell walls and more effective removal of reactive oxygen species (ROS) from the plant. The levels of certain metabolites only increased in Ludan No. 8, indicating that Pb-sensitive varieties may use different metabolic pathways to cope with Pb stress. Both varieties showed increased levels of some metabolites related to antioxidant protection and osmotic regulation. This study provides an understanding of maize Pb tolerance mechanisms and a basis for further development of tools for use in maize breeding.
AtCRY2 Negatively Regulates the Functions of AtANN2 and AtANN3 in Drought Tolerance by Affecting Their Subcellular Localization and Transmembrane Ca2+ Flow
Authors: Liu Ting , Du Leyan , Li Qiushi , Kang Jingda , Guo Qi , Wang Shilin
Published: November 2021

Annexins are a multifunctional class of calcium-binding proteins in plants, and their physiological functions and regulation in response to drought stress remain to be elucidated. Here, we found that AtANN2 and AtANN3 conferred to drought tolerance under short-day and long-day conditions, respectively. Under their functional photoperiod, AtANN2 and AtANN3 gene expression was enhanced in the mannitol-treated roots, and their encoded proteins were rapidly targeted to the plasma membrane, and mediated significant Ca2+ flows across the plasma membrane. Cryptochromes as photoreceptors can not only sense the photoperiod and regulate ion channels on the plasma membrane to influence ion flow but also induce downstream physiological responses. AtCRY2 repressed the functions of AtANN2 and AtANN3 by affecting their plasma membrane localization and inhibited AtANN2- and AtANN3-dependent transmembrane Ca2+ flow in response to drought stress. Taken together, these results uncover a mechanism linking Annexins-AtCRY2 to transmembrane Ca2+ flow and resulting in enhanced drought tolerance in Arabidopsis.
Sage Insights Into the Phylogeny of Salvia: Dealing With Sources of Discordance Within and Across Genomes
Authors: Rose Jeffrey P. , Kriebel Ricardo , Kahan Larissa , DiNicola Alexa , González-Gallegos Jesús G. , Celep Ferhat , Lemmon Emily M. , Lemmon Alan R. , Sytsma Kenneth J. , Drew Bryan T.
Published: November 2021

Next-generation sequencing technologies have facilitated new phylogenomic approaches to help clarify previously intractable relationships while simultaneously highlighting the pervasive nature of incongruence within and among genomes that can complicate definitive taxonomic conclusions. Salvia L., with ∼1,000 species, makes up nearly 15% of the species diversity in the mint family and has attracted great interest from biologists across subdisciplines. Despite the great progress that has been achieved in discerning the placement of Salvia within Lamiaceae and in clarifying its infrageneric relationships through plastid, nuclear ribosomal, and nuclear single-copy genes, the incomplete resolution has left open major questions regarding the phylogenetic relationships among and within the subgenera, as well as to what extent the infrageneric relationships differ across genomes. We expanded a previously published anchored hybrid enrichment dataset of 35 exemplars of Salvia to 179 terminals. We also reconstructed nearly complete plastomes for these samples from off-target reads. We used these data to examine the concordance and discordance among the nuclear loci and between the nuclear and plastid genomes in detail, elucidating both broad-scale and species-level relationships within Salvia. We found that despite the widespread gene tree discordance, nuclear phylogenies reconstructed using concatenated, coalescent, and network-based approaches recover a common backbone topology. Moreover, all subgenera, except for Audibertia, are strongly supported as monophyletic in all analyses. The plastome genealogy is largely resolved and is congruent with the nuclear backbone. However, multiple analyses suggest that incomplete lineage sorting does not fully explain the gene tree discordance. Instead, horizontal gene flow has been important in both the deep and more recent history of Salvia. Our results provide a robust species tree of Salvia across phylogenetic scales and genomes. Future comparative analyses in the genus will need to account for the impacts of hybridization/introgression and incomplete lineage sorting in topology and divergence time estimation.
Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
Authors: Hwang Geonhee , Park Jeeyoon , Kim Soohwan , Park Jeonghyang , Seo Dain , Oh Eunkyoo
Published: November 2021

Thermomorphogenesis is the morphological response of plants to an elevation in the ambient temperature, which is mediated by the bHLH transcription factor PIF4. The evening-expressed clock component, PRR5, directly represses the expression of PIF4 mRNA. Additionally, PRR5 interacts with PIF4 protein and represses its transactivation activity, which in turn suppresses the thermoresponsive growth in the evening. Here, we found that the B-box zinc finger protein, BBX18, interacts with PRR5 through the B-Box2 domain. Deletion of the B-Box2 domain abolished the functions of BBX18, including the stimulation of PIF4 mRNA expression and hypocotyl growth. Overexpression of BBX18, and not of B-Box2-deleted BBX18, restored the expression of thermoresponsive genes in the evening. We further show that BBX18 prevents PRR5 from inhibiting PIF4-mediated high temperature responses. Taken together, our results suggest that BBX18 regulates thermoresponsive growth through the PRR5-PIF4 pathway.
Lipidomic, Transcriptomic, and BSA-660K Single Nucleotide Polymorphisms Profiling Reveal Characteristics of the Cuticular Wax in Wheat
Authors: Zheng Jun , Yang Chenkang , Zheng Xingwei , Yan Suxian , Qu Fei , Zhao Jiajia , Pei Yanxi
Published: November 2021

Plant epidermal wax helps protect plants from adverse environmental conditions, maintains the function of tissues and organs, and ensures normal plant development. However, the constituents of epidermal wax and the regulatory mechanism of their biosynthesis in wheat have not been fully understood. Wheat varieties with different wax content, Jinmai47 and Jinmai84, were selected to comparatively analyze their waxy components and genetic characteristics, using a combination of lipidomic, transcriptomic, and BSA-Wheat 660K chip analysis. Through lipidomic analysis, 1287 lipid molecules were identified representing 31 lipid subclasses. Among these, Diacylglycerols (DG), (O-acyl)-ω-hydroxy fatty acids (OAHFA), wax ester (WE), Triacylglycerols (TG), and Monoradylglycerols (MG) accounted for 96.4% of the total lipids in Jinmai84 and 94.5% in Jinmai47. DG, OAHFA, and WE were higher in Jinmai84 than in Jinmai47 with the content of OAHFA 2.88-fold greater and DG 1.66-fold greater. Transcriptome sequence and bioinformatics analysis revealed 63 differentially expressed genes related to wax biosynthesis. Differentially expressed genes (DEGs) were found to be involved with the OAHFA, DG, and MG of synthesis pathways, which enriched the wax metabolism pathway. Non-glaucous and glaucous bulks from a mapping population were used to identify single nucleotide polymorphisms (SNP) via 660K chip analysis. Two loci centered on chromosomes 2D and 4B were detected and the locus on 4B is likely novel. These data improve understanding of complex lipid metabolism for cuticular wax biosynthesis in wheat and lay the foundation for future detailed investigation of mechanisms regulating wax metabolism.
A Comparative Analysis of Selection Criteria of Candidates in Belgium
Authors: Vandeleene Audrey , van Haute Emilie
Published: November 2021

The literature on candidate selection has focussed extensively on the degree of inclusiveness and decentralization of the selectorate, as part of the debate on intra-party democracy. However, much less attention has been paid to the degree of openness of candidacies, or selection criteria within parties. Yet parties have a lot of leeway in how they design selection criteria internally. Which guidelines do parties follow when making the crucial choice on which candidates to select for elections? This paper investigates selection criteria from two perspectives: the formal rules set by parties that restrict the candidate’s pool and the (informal) preferences of selectors that shape who gets selected. We aim first at contrasting the degree of party institutionalization and parties’ formal rules in candidate selection and so, we shed light on whether parties formalise their candidacy requirements and candidate selection processes to the same extent as other party activities. Second, the paper investigates the role of the selectorates, and how selectorate’s characteristics matter for the kind of (informal) selection criteria, be they intended at maximizing offices, votes or policies. Drawing on party statutes coded in the Political Party Database (PPDB) and 23 in-depth interviews with selectors, we study three francophone Belgian parties that differ both in terms of inclusiveness of the selectorate who has the final say on candidate selection and in terms of degree of centralisation, and in terms of party institutionalisation: the green party (Ecolo), the socialist party (PS), and the liberal party (MR). Our comparative analysis of parties, selection criteria provides new insights into the secret garden of politics and highlights in particular the major impact of parties, degree of centralization.
The Effects of Using a Nature-Sound Mobile Application on Psychological Well-Being and Cognitive Performance Among University Students
Authors: Luo Jiutong , Wang Minhong , Chen Ling
Published: November 2021

Many university students have been struggling with multiple challenges that may cause mental fatigue. Exposure to the natural environment is found to have restorative effects on mental fatigue, which can be explained by its benefits in physiological, psychological, and cognitive aspects. While the natural environment contains both visual and auditory elements, research on the effects of auditory elements, such as nature sounds, is underdeveloped and limited to laboratory settings. It remains unclear what are the effects of exposure to nature sounds in daily life settings. The study was conducted with 71 students from a university, who were randomly assigned to the experimental group using a nature-sound mobile application in daily life and the control group not using the application. After a 4-week exposure to the intervention, the students in the experimental group outperformed their counterparts in the control group on psychological well-being reflected in positive affect, as well as cognitive performance reflected in flow state, attention (in terms of alerting) and working memory (in terms of accuracy and reaction time). The findings reveal the positive impact of exposure to relaxing nature sounds on university students’ psychological well-being and cognitive performance, as well as the potential of mobile applications to provide easy exposure to nature sounds.
The Unified Protocol for Transdiagnostic Treatment of Emotional Disorders Among Japanese Children: A Pilot Study
Authors: Fujisato Hiroko , Kato Noriko , Namatame Hikari , Ito Masaya , Usami Masahide , Nomura Tomoko , Ninomiya Shuzo , Horikoshi Masaru
Published: November 2021

At present, there is no established cognitive behavioral therapy (CBT) for treating emotional disorders in Japanese children. Therefore, we introduced the Unified Protocol for Transdiagnostic Treatment of Emotional Disorders in Children (UP-C) in Japan and adapted it to the Japanese context. We then examined its feasibility and preliminary efficacy using a single-arm pretest, posttest, follow-up design. Seventeen Japanese children aged between 8 and 12 years (female n = 11; male n = 6; M = 10.06 ± 0.97 years) with a principal diagnosis of anxiety, obsessive-compulsive, or depressive disorders, and their parents were enrolled in the study. The primary outcome was the overall severity of emotional disorders as assessed by psychiatrists using the Clinical Global Impression-Severity Scale. Secondary outcomes included child- and parent-reported anxiety symptoms, depressive symptoms, and functional status. No severe adverse events were observed. The feasibility was confirmed by the low dropout proportion (11.76%), high attendance proportion (children: 95.6%; parents: 94.6%), and sufficient participant satisfaction. Linear mixed models (LMMs) showed that the overall severity of emotional disorders and child- and parent-reported anxiety symptoms improved from pre-treatment to post-treatment, and that these treatment effects were maintained during the 3-month follow-up period. Additionally, child- and parent-reported functional status improved from pre-treatment to the 3-month follow-up. In contrast, child-reported depressive symptoms improved from pre-treatment to follow-up, but there was no significant change in parent-reported depressive symptoms between pre-treatment and other time points. These findings demonstrate the feasibility and preliminary efficacy of the Japanese version of the UP-C, suggesting that future randomized controlled trials (RCTs) are warranted (Clinical trial registration: UMIN000026911).
Occupational Well-Being in Beginning Early Childhood Educators of Hong Kong and the Prediction of Job-Related Factors: Variable-Centered and Person-Centered Approaches
Authors: Li Jian-Bin , Yang An , Zhang Rui , Leung Tuen Yung , Li Zhouxing
Published: November 2021

The occupational well-being (OWB) of early childhood educators (ECEs) play a crucial role in their job performance, the development of a child, and the operation of early childhood education sectors. OWB of ECEs has been an increasing concern in recent years and this concern might be particularly salient for beginning ECEs given the multiple adaptive changes and challenges they encounter during the transition from training to teaching. However, research on the beginning ECEs’ OWB has been scarce. In this study, we employed both variable-centered and person-centered approaches to explore OWB and examined job demands and resources as predictors in 117 Hong Kong beginning ECEs (113 females, Mage = 21.71 years). They first reported job demands (i.e., class size, working hours, dealing with children with special education needs) and job resources (i.e., salary and job support) at the end of the first month of the fall semester upon working as in-service teachers. They then reported on four OWB variables (i.e., job satisfaction, work engagement, job stress, and job burnout) at the beginning of the spring semester. Results of the variable-centered analysis revealed that beginning ECEs reported medium or above-medium levels on the positive OWB indicators and medium or below-medium levels on the negative OWB indicators. Regression analysis found that despite some exceptions, job demands and job resources negatively (positively) and positively (negatively) predicted positive (negative) OWB indicators, respectively. Results of person-centered analysis suggested that the complex pattern of different OWB indicators could be categorized into two OWB profiles (medium well-being vs. relatively weak well-being – emotional exhaustion). Results of regression analysis showed that beginning ECEs with higher job demands were less likely, whereas those with more job resources were more likely, to be assigned to the medium well-being profile. These results inform which leverage points could be targeted to enhance a specific OWB indicator and identify who would be in dire need to enhance their OWB at the very beginning of their teaching career.
The Effects of Tai Chi and Qigong Exercise on Psychological Status in Adolescents: A Systematic Review and Meta-Analysis
Authors: Liu Xuan , Li Ru , Cui Jiabao , Liu Fang , Smith Lee , Chen Xiaorong , Zhang Debao
Published: November 2021

Background: The purpose of this study was to systematically review the effectiveness of Tai Chi and Qigong exercise on adolescents' symptoms of depression and anxiety, and psychological status based on clinical evidences, and to calculate the pooled results using meta-analysis.

Methods: A systematic search using seven English and three Chinese databases was initiated to identify randomized controlled trials (RCT) and non-randomized comparison studies (NRS) assessing the effect of Tai Chi and Qigong exercise on psychological status among adolescents. Standardized mean differences (SMD) and their 95% confidence intervals (CI) were used to determine the pooled effect of the intervention. Study quality was evaluated using a Checklist to Evaluate a Report of a Non-pharmacological Trial (CLEAR-NPT) designed for non-pharmacological trials.

Results: Four RCTs and six NRS were identified, including 1,244 adolescents. The results suggested a potential beneficial effect of Tai chi and Qigong exercise on reducing anxiety (SMD = 0.386, 95 CI% [0.233, 0.538]) and depression (SMD = 1.937 [95 CI%, 1.392–2.546]) symptoms, and reducing cortisol level (SMD = 0.621 [95 CI%, 0.18–1.062]) in adolescents. Conversely, non-significant effects were found for stress, mood, and self-esteem.

Conclusions: The findings of this review suggest Qigong appears to be an effective therapeutic modality to improve psychological well-being in adolescents. Hope future studies will have rigorously designed, well-controlled randomized trials with large sample sizes in order to confirm these findings.
Individual Differences and Susceptibility to Burnout Syndrome: Sensory Processing Sensitivity and Its Relation to Exhaustion and Disengagement
Authors: Golonka Krystyna , Gulla Bożena
Published: November 2021

The aim of the study is to analyze the relationship between sensory processing sensitivity and occupational burnout. This analysis makes it possible to explore the problem of sensitivity as a predictor of burnout syndrome and to discuss adequate coping strategies in the work environment to improve employee well-being. The sample consists of 516 employees (236 women; Mage = 28.97, SD = 6.62 years). The Highly Sensitive Person Scale (HSPS; 27 items) was used to measure sensory processing sensitivity; the Oldenburg Burnout Inventory (OLBI; 16 items) was used to measure exhaustion and disengagement from work. The analysis of sensitivity is based on the ‘DOES’ model of a highly sensitive person, which includes depth of processing (D), overstimulation (O), emotional reactivity and empathy (E), and sensing the subtle (S). Burnout due to exhaustion and disengagement is analyzed. The factor analysis of the HSPS confirms its three-factor structure. The results indicate significant relationships between sensory processing sensitivity and burnout symptoms. However, the influence of specific aspects of sensitivity on the burnout problem varies: the results indicate that higher emotional reactivity is linked to worse burnout symptoms, but sensing the subtle shows the opposite effect and may be a protective factor against exhaustion. Significant gender differences were observed [F(511,1) = 136.63, p < 0.001; η2 = 0.21]; women revealed a significantly higher level of general sensitivity (Mwomen = 4.66, SD = 0.62) as compared to men (Mmen = 4.01; SD = 0.64). Awareness of one’s high sensory-processing sensitivity and its potential outcomes in the work environment may be essential in order to implement appropriate regulatory strategies. Proactive strategies aimed at reducing prolonged emotional overload may be critical for highly sensitive employees. Recognizing high sensitivity may reduce burnout symptoms and improve well-being at work.
Professional Support After Partner Loss: Likelihood and Correlates of Help-Seeking Behavior
Authors: Jopp Daniela S. , Lampraki Charikleia , Meystre Claudia , Znoj Hansjörg , Brodbeck Jeannette
Published: November 2021

Intimate partner loss in later life can be one of the most stressful events in adulthood. Individuals who struggle to adapt to the new life conditions may need support from a mental health professional. However, less is known about the likelihood to seek professional help after separation, divorce, or bereavement in later life and associated factors. This study investigated professional help-seeking (PHS) for partner loss after a long-term marriage in separated, divorced, and bereaved individuals and examined the extent to which specific person and event-related variables, as well as depressive symptoms, increase its likelihood. The data were derived from the LIVES “Intimate Partner Loss Study.” The self-administered questionnaires were completed by 388 adults. PHS was higher after separation (57%) and divorce (49%), compared to widowhood (18%). Higher likelihood of PHS was associated with separation and divorce, female gender, having someone to count on, loss unexpectedness, needing more time to overcome the loss, and more depressive symptoms. Informing individuals unlikely to seek help (e.g., males, bereaved, and individuals with no confidant) about PHS benefits may facilitate adaptation to partner loss.
Implementation and Evaluation of Therapeutic Online Coaching Using Habit Reversal Training in Children With Tourette’s Disorder – A Pilot Study
Authors: Viefhaus Paula , Adam Julia , Goletz Hildegard , Woitecki Katrin , Döpfner Manfred
Published: November 2021

Cognitive-behavioral interventions can be difficult to implement in daily routine, which is often essential for generalizing treatment effects to natural settings. Furthermore, there is a lack of adequate care options concerning habit reversal training for children with Tourette’s disorder. The objective of this study is to evaluate therapeutic online coachingviavideoconferencing in the natural environment of children with Tourette’s disorder in addition to face-to-face therapy (blended therapy). Online coaching took place twice a week for a maximum of 12 weeks. In a single-case study (n= 5; patients aged 8–11 years), the first results were obtained for exploratory purposes, especially with regard to the feasibility and reduction of symptoms and impairment. Various outcome measures were assessed (severity of symptoms, impairment, practical implementation, and satisfaction). Despite some principal limitations, the findings provide first hints that blended therapy is feasible and improves symptoms in some children with tics.

Clinical Trial Registration:[https://clinicaltrials.gov/], identifier [DRKS00017199].
Associations Between Psychological and Immunological Variables in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis: A Systematic Review
Authors: Raanes Emilie F. W. , Stiles Tore C.
Published: November 2021

Background: Little emphasis has been given to the fact that various psychological processes and behaviors in chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) have neural correlates that affect—and are affected by—the immune system. The aim of this paper is to provide a systematic review of the literature on cross-sectional and longitudinal associations between psychological and immunological variables/changes in CFS/ME.

Methods: The systematic literature search was conducted on Dec 10, 2020 using PubMed. Original research studies investigating associations between a predefined set of psychological and immunological variables in CFS/ME were included. Specifically, the review was focused on studies examining the following psychological variables: executive function, emotion regulation, interpersonal function, sleep, mental health, anxiety, depression, and/or other psychiatric symptoms. In terms of immunological variables, studies investigating interleukin (IL)-1, IL-2, IL-4, IL-6, tumor necrosis factor (TNF), CD4+, and/or CD8+ were included. Besides original research papers, other potentially relevant papers (e.g., literature reviews) were carefully read and reference lists were checked in order to identify any additional relevant studies. Available data was summarized in text and tables.

Results: The literature search identified 897 potentially relevant papers. Ultimately, 14 studies (807 participants in total) were included in the review of which only two were longitudinal in nature. The review indicated that executive function is associated with IL-1 and IL-6, and interpersonal function is associated with IL-6 and TNF-α. Further, the available data suggested that emotion regulation is associated with IL-2 and sleep is associated with IL-1, IL-6, TNF-α, and IL-2. Interestingly, poorer emotion regulation, interpersonal function, and sleep have all been found to be associated with higher cytokine levels. Executive function has shown both positive and negative relationships with cytokines and among these psychological constructs, it is also the only one that has been found to be associated with CD4+ and CD8+ counts/percentages.

Conclusions: Correlations exist between psychological and immunological variables in CFS/ME. However, there are few consistent findings and there is almost a complete lack of longitudinal studies. This review points to a gap in existing CFS/ME research and hopefully, it will inspire to the generation of innovative, psychoneuroimmunological hypotheses within the CFS/ME research field.
A Family-Focused Intervention for Parental Mental Illness: A Practitioner Perspective
Authors: Furlong Mairead , Mulligan Christine , McGarr Sharon , O'Connor Siobhan , McGilloway Sinead
Published: November 2021

Background: Parental mental illness (PMI) is common and can lead to children developing mental disorders. Family Talk (FT) is a well-known and widely implemented intervention designed to reduce the risk of transgenerational psychopathology. However, given the research to practise “gap,” very little qualitative research, to date, has investigated practitioner experiences in implementing FT. This study aimed to explore the practitioner-perceived barriers and facilitators to the implementation and sustainability of FT within mainstream mental health settings.

Methods: This qualitative study was nested within a randomised controlled trial (RCT) of Family Talk [N = 86 families (139 parents, 221 children)] within 15 adult (AMHS), child (CAMHS), primary care mental health, and child protection sites in Ireland. Semi-structured interviews and focus groups were undertaken with a purposive sample of clinicians (n = 31) and managers (n = 10), based on their experiences of implementing FT. Interview data were transcribed verbatim, analysed using constructivist grounded theory, and informed by Fixsen's implementation science framework.

Results: Service providers highlighted a number of benefits for approximately two thirds of families across different diagnoses and mental health settings (AMHS/CAMHS/primary care). Sites varied in their capacity to embed FT, with key enablers identified as acquiring managerial and organisational support, building clinician skill, and establishing interagency collaboration. Implementation challenges included: recruitment difficulties, stresses in working with multiply-disadvantaged families, disruption in delivery due to the COVID-19 global pandemic, and sustainability concerns (e.g., perceived fit of FT with organisational remit/capacity, systemic and cultural barriers to change).

Conclusion: This study is only the second qualitative study ever conducted to explore practitioner experiences in implementing FT, and the first conducted within the context of an RCT and national research programme to introduce family-focused practise (FFP) for families living with PMI. The findings illuminate the successes and complexities of implementing FFP in a country without a “think family” infrastructure, whilst highlighting a number of important generalisable lessons for the implementation of FT, and other similar interventions, elsewhere.
Therapeutic Reference Ranges for Psychotropic Drugs: A Protocol for Systematic Reviews
Authors: Hart Xenia M. , Eichentopf Luzie , Lense Xenija , Riemer Thomas , Wesner Katja , Hiemke Christoph , Gründer Gerhard
Published: November 2021

Background: For many psychotropic drugs, monitoring of drug concentrations in the blood (Therapeutic Drug Monitoring; TDM) has been proven useful to individualize treatments and optimize drug effects. Clinicians hereby compare individual drug concentrations to population-based reference ranges for a titration of prescribed doses. Thus, established reference ranges are pre-requisite for TDM. For psychotropic drugs, guideline-based ranges are mostly expert recommendations derived from a conglomerate of cohort and cross-sectional studies. A systematic approach for identifying therapeutic reference ranges has not been published yet. This paper describes how to search, evaluate and grade the available literature and validate published therapeutic reference ranges for psychotropic drugs.

Methods/Results: Following PRISMA guidelines, relevant databases have to be systematically searched using search terms for the specific psychotropic drug, blood concentrations, drug monitoring, positron emission tomography (PET) and single photon emission computed tomography (SPECT). The search should be restricted to humans, and diagnoses should be pre-specified. Therapeutic references ranges will not only base upon studies that report blood concentrations in relation to clinical effects, but will also include implications from neuroimaging studies on target engagement. Furthermore, studies reporting concentrations in representative patient populations are used to support identified ranges. Each range will be assigned a level of underlying evidence according to a systematic grading system.

Discussion: Following this protocol allows a comprehensive overview of TDM literature that supports a certain reference range for a psychotropic drug. The assigned level of evidence reflects the validity of a reported range rather than experts' opinions.
The Organic Turn: Coping With Pandemic and Non-pandemic Challenges by Integrating Evidence-, Theory-, Experience-, and Context-Based Knowledge in Advising Health Policy
Authors: Pfaff Holger , Schmitt Jochen
Published: November 2021

The COVID-19 pandemic has posed an extraordinary challenge for public health and health policy. Questions have arisen concerning the main strategies to cope with this situation and the lessons to be learned from the pandemic. This conceptual paper aims to clarify these questions via sociological concepts. Regarding coping strategies used during the pandemic, there is a strong tendency for health policymakers to rely on expert knowledge rather than on evidence-based knowledge. This has caused the evidence-based healthcare community to respond to urgent demands for advice by rapidly processing new knowledge. Nonetheless, health policymakers still mainly rely on experts in making policy decisions. Our sociological analysis of this situation identified three lessons for coping with pandemic and non-pandemic health challenges: (1) the phenomenon of accelerating knowledge processing could be interpreted from the organizational innovation perspective as a shift from traditional mechanistic knowledge processing to more organic forms of knowledge processing. This can be described as an “organic turn.” (2) The return of experts is part of this organic turn and shows that experts provide both evidence-based knowledge as well as theoretical, experiential, and contextual knowledge. (3) Experts can use theory to expeditiously provide advice at times when there is limited evidence available and to provide complexity-reducing orientation for decisionmakers at times where knowledge production leads to an overload of knowledge; thus, evidence-based knowledge should be complemented by theory-based knowledge in a structured two-way interaction to obtain the most comprehensive and valid recommendations for health policy.
Global Burden of Respiratory Diseases Attributable to Ambient Particulate Matter Pollution: Findings From the Global Burden of Disease Study 2019
Authors: Wu Ying , Song Ping , Lin Shuai , Peng Ling , Li Yizhen , Deng Yujiao , Deng Xinyue , Lou Weiyang , Yang Si , Zheng Yi , Xiang Dong , Hu Jingjing , Zhu Yuyao , Wang Meng , Zhai Zhen , Zhang Dai , Dai Zhijun , Gao Jie
Published: November 2021

Background: Exposure to ambient particulate matter pollution (APMP) is a global health issue that directly affects the human respiratory system. Thus, we estimated the spatiotemporal trends in the burden of APMP-related respiratory diseases from 1990 to 2019.

Methods: Based on the Global Burden of Disease Study 2019, data on the burden of APMP-related respiratory diseases were analyzed by age, sex, cause, and location. Joinpoint regression analysis was used to analyze the temporal trends in the burden of different respiratory diseases over the 30 years.

Results: Globally, in 2019, APMP contributed the most to chronic obstructive pulmonary disease (COPD), with 695.1 thousand deaths and 15.4 million disability-adjusted life years (DALYs); however, the corresponding age-standardized death and DALY rates declined from 1990 to 2019. Similarly, although age-standardized death and DALY rates since 1990 decreased by 24% and 40%, respectively, lower respiratory infections (LRIs) still had the second highest number of deaths and DALYs attributable to APMP. This was followed by tracheal, bronchus, and lung (TBL) cancer, which showed increased age-standardized death and DALY rates during the past 30 years and reached 3.78 deaths per 100,000 persons and 84.22 DALYs per 100,000 persons in 2019. Among children aged < 5 years, LRIs had a huge burden attributable to APMP, whereas for older people, COPD was the leading cause of death and DALYs attributable to APMP. The APMP-related burdens of LRIs and COPD were relatively higher among countries with low and low-middle socio-demographic index (SDI), while countries with high-middle SDI showed the highest burden of TBL cancer attributable to APMP.

Conclusions: APMP contributed substantially to the global burden of respiratory diseases, posing a significant threat to human health. Effective actions aimed at air pollution can potentially avoid an increase in the PM2.5-associated disease burden, especially in highly polluted areas.
Application of SARS-CoV-2 Serology to Address Public Health Priorities
Authors: Sherman Amy C. , Smith Teresa , Zhu Yerun , Taibl Kaitlin , Howard-Anderson Jessica , Landay Taylor , Pisanic Nora , Kleinhenz Jennifer , Simon Trevor W. , Espinoza Daniel , Edupuganti Neena , Hammond Skyler , Rouphael Nadine , Shen Huifeng , Fairley Jessica K. , Edupuganti Srilatha , Cardona-Ospina Jaime A. , Rodriguez-Morales Alfonso J. , Premkumar Lakshmanane , Wrammert Jens , Tarleton Rick , Fridkin Scott , Heaney Christopher D. , Scherer Erin M. , Collins Matthew H.
Published: November 2021

Background: Antibodies against SARS-CoV-2 can be detected by various testing platforms, but a detailed understanding of assay performance is critical.

Methods: We developed and validated a simple enzyme-linked immunosorbent assay (ELISA) to detect IgG binding to the receptor-binding domain (RBD) of SARS-CoV-2, which was then applied for surveillance. ELISA results were compared to a set of complimentary serologic assays using a large panel of clinical research samples.

Results: The RBD ELISA exhibited robust performance in ROC curve analysis (AUC> 0.99; Se = 89%, Sp = 99.3%). Antibodies were detected in 23/353 (6.5%) healthcare workers, 6/9 RT-PCR-confirmed mild COVID-19 cases, and 0/30 non-COVID-19 cases from an ambulatory site. RBD ELISA showed a positive correlation with neutralizing activity (p = <0.0001, R2 = 0.26).

Conclusions: We applied a validated SARS-CoV-2-specific IgG ELISA in multiple contexts and performed orthogonal testing on samples. This study demonstrates the utility of a simple serologic assay for detecting prior SARS-CoV-2 infection, particularly as a tool for efficiently testing large numbers of samples as in population surveillance. Our work also highlights that precise understanding of SARS-CoV-2 infection and immunity at the individual level, particularly with wide availability of vaccination, may be improved by orthogonal testing and/or more complex assays such as multiplex bead assays.
Global Industrial Development: Insights From the Distribution Dynamics Approach for the Post Covid Era
Authors: Ma Ning , Shum Wai Yan , Han Tingting , Cheong Tsun Se
Published: November 2021

The spread of COVID-19 has significantly dampened global economic activity and has also wreaked havoc on the industrial sector. Understanding the disparity and convergence of global industrial outputs is important in assessing the effectiveness of concurrent development policies. This study investigates the spatial distribution of global industrial output to unveil the disparity in industrial development and the feasibility of achieving convergence over time. Stochastic kernel analyses are carried out for national regimes to study the overall pattern of industrialization for all the countries in the world. Countries are then classified into different groups to further analyse the geographical and income effects on industrial development. The results show that disparity between the Global North and the Global South will enlarge further in the future. Industrial development in the Global North will continue to prosper, while the industrial output in many countries in the Global South just cannot reach the global average.
Cultivating Pedagogy for Transformative Learning: A Decade of Undergraduate Agroecology Education
Authors: Horner Catherine E. , Morse Cheryl , Carpenter Nell , Nordstrom Karen L. , Faulkner Joshua W. , Mares Teresa , Kinnebrew Eva , Caswell Martha , Izzo Victor , Méndez V. Ernesto , Lewins Scott A. , McCune Nils
Published: November 2021

Existing scholarship on agroecology and food systems education within U.S. colleges and universities has focused primarily on preparing students to be professionals working in agrifood systems. Developing students' skills and competencies, though vitally important, may not suffice for supporting transformative learning. Transformative learning shifts students' perceptions and awareness and informs future actions, constituting a potential avenue for leveraging education to support transformations toward more socially just and ecologically viable agrifood systems. It is unclear, however, what pedagogies and educational practices enable transformative learning. This paper explores the integration of multiple pedagogical innovations within an advanced agroecology course taught at the University of Vermont. Over a decade, the teaching team has made iterative adjustments to course content and pedagogies with the goal of catalyzing action toward transforming agrifood systems. In this paper, we evaluate our pedagogical approach, asking: (1) How well do course content and pedagogy align with our definition of transformative agroecology as transdisciplinary, participatory, action-oriented, and political? (2) How well does our approach enable transformative agroecological learning, and how is that identified? We present our course evaluation as a case study comprising qualitative analyses of course syllabi, student comments on University-administered course evaluations, and most significant change (MSC) reflections. MSC reflections proved to be a valuable method for identifying and assessing transformative learning. Through a curricular review, we found that substantial changes to course content and evaluative assignments between 2010 and 2020 align with a transformative approach to agroecology. This is validated in students' MSC reflections, which provide evidence of transformative learning. In sharing evaluative results, processes, and insights, we aim to contribute to a broader movement of scholar educators committed to iteratively and collaboratively developing transformative pedagogies within agroecology and sustainable food system education. We contend that reflexive practice among educators is necessary to leverage education for transforming agrifood systems.
SNP and Phylogenetic Characterization of Low Viral Load SARS-CoV-2 Specimens by Target Enrichment
Authors: Orf Gregory S. , Forberg Kenn , Meyer Todd V. , Mowerman Illya , Mohaimani Aurash , Faron Matthew L. , Jennings Cheryl , Landay Alan L. , Goldstein D. Yitz , Fox Amy S. , Berg Michael G. , Cloherty Gavin A.
Published: November 2021

Background: Surveillance of SARS-CoV-2 across the globe has enabled detection of new variants and informed the public health response. With highly sensitive methods like qPCR widely adopted for diagnosis, the ability to sequence and characterize specimens with low titers needs to keep pace.

Methods: Nucleic acids extracted from nasopharyngeal swabs collected from four sites in the United States in early 2020 were converted to NGS libraries to sequence SARS-CoV-2 genomes using metagenomic and xGen target enrichment approaches. Single nucleotide polymorphism (SNP) analysis and phylogeny were used to determine clade assignments and geographic origins of strains.

Results: SARS-CoV-2-specific xGen enrichment enabled full genome coverage for 87 specimens with Ct values <29, corresponding to viral loads of >10,000 cp/ml. For samples with viral loads between 103 and 106 cp/ml, the median genome coverage for xGen was 99.1%, sequence depth was 605X, and the “on-target” rate was 57 ± 21%, compared to 13%, 2X and 0.001 ± 0.016%, respectively, for metagenomic sequencing alone. Phylogenetic analysis revealed the presence of most clades that existed at the time of the study, though clade GH dominated in the Midwest.

Conclusions: Even as vaccines are being widely distributed, a high case load of SARS-CoV-2 infection persists around the world. Viral genetic surveillance has succeeded in warning the public of new variants in circulation and ensured that diagnostic tools remain resilient to a steadily increasing number of mutations. Target capture offers a means of characterizing low viral load samples which would normally pose a challenge for metagenomic sequencing.
Isolation and Identification Rust Pathogens and the Study of Antioxidant Enzyme Activity and Gene Expression under Rust Infection in Zoysia japonica
Authors: Zhang Di , Zeng Hanguo , Zhao Liyuan , Yue Jiaming , Qi Xiao , Li Manli
Published: November 2021

The goal of this study was to identify the zoysiagrass rust pathogens and to analyze the differences in rust-resistant and rust-susceptible Zoysia japonica germplasm upon inoculation. Based on the assessment of spore morphology and 18S ribosomal DNA (rDNA) molecular identification, the zoysiagrass rust pathogen was identified as Puccinia zoysiae Diet. The development of mycelium, the rate of spreading, and the timing of spore production were more delayed in the rust-resistant (RR) genotype than the rust-susceptible (RS) genotype. After inoculation, the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) initially decreased, then increased in both the RR and RS genotypes, but the increased enzyme activities were faster in the RR than in the RS genotype. Rust resistance was positively correlated with antioxidant enzyme activity. The observed changes in CAT, POD and APX activity corresponded to their gene expression levels. The results of this study may be utilized in accurately evaluating the damage of rust disease and rust-resistance in zoysiagrass germplasm aimed at breeding the rust-resistant zoysiagrass varieties and improving disease management of zoysiagrass turf.