bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Regions of Local Recurrence in Glioblastomas Using Voxel-Based Radiomic Features of Multiparametric Postoperative MRI
Authors: Cepeda Santiago , Luppino Luigi Tommaso , Pérez-Núñez Angel , Solheim Ole , García-García Sergio , Velasco-Casares María , Karlberg Anna , Eikenes Live , Sarabia Rosario , Arrese Ignacio , Zamora Tomás , Gonzalez Pedro , Jiménez-Roldán Luis , Kuttner Samuel
Published: March 2023

The globally accepted surgical strategy in glioblastomas is removing the enhancing tumor. However, the peritumoral region harbors infiltration areas responsible for future tumor recurrence. This study aimed to evaluate a predictive model that identifies areas of future recurrence using a voxel-based radiomics analysis of magnetic resonance imaging (MRI) data. This multi-institutional study included a retrospective analysis of patients diagnosed with glioblastoma who underwent surgery with complete resection of the enhancing tumor. Fifty-five patients met the selection criteria. The study sample was split into training (N = 40) and testing (N = 15) datasets. Follow-up MRI was used for ground truth definition, and postoperative structural multiparametric MRI was used to extract voxel-based radiomic features. Deformable coregistration was used to register the MRI sequences for each patient, followed by segmentation of the peritumoral region in the postoperative scan and the enhancing tumor in the follow-up scan. Peritumoral voxels overlapping with enhancing tumor voxels were labeled as recurrence, while non-overlapping voxels were labeled as nonrecurrence. Voxel-based radiomic features were extracted from the peritumoral region. Four machine learning-based classifiers were trained for recurrence prediction. A region-based evaluation approach was used for model evaluation. The Categorical Boosting (CatBoost) classifier obtained the best performance on the testing dataset with an average area under the curve (AUC) of 0.81 ± 0.09 and an accuracy of 0.84 ± 0.06, using region-based evaluation. There was a clear visual correspondence between predicted and actual recurrence regions. We have developed a method that accurately predicts the region of future tumor recurrence in MRI scans of glioblastoma patients. This could enable the adaptation of surgical and radiotherapy treatment to these areas to potentially prolong the survival of these patients.
DNA Repair and Therapeutic Strategies in Cancer Stem Cells
Authors: Gillespie Matthew S. , Ward Ciara M. , Davies Clare C.
Published: March 2023

First-line cancer treatments successfully eradicate the differentiated tumour mass but are comparatively ineffective against cancer stem cells (CSCs), a self-renewing subpopulation thought to be responsible for tumour initiation, metastasis, heterogeneity, and recurrence. CSCs are thus presented as the principal target for elimination during cancer treatment. However, CSCs are challenging to drug target because of numerous intrinsic and extrinsic mechanisms of drug resistance. One such mechanism that remains relatively understudied is the DNA damage response (DDR). CSCs are presumed to possess properties that enable enhanced DNA repair efficiency relative to their highly proliferative bulk progeny, facilitating improved repair of double-strand breaks induced by radiotherapy and most chemotherapeutics. This can occur through multiple mechanisms, including increased expression and splicing fidelity of DNA repair genes, robust activation of cell cycle checkpoints, and elevated homologous recombination-mediated DNA repair. Herein, we summarise the current knowledge concerning improved genome integrity in non-transformed stem cells and CSCs, discuss therapeutic opportunities within the DDR for re-sensitising CSCs to genotoxic stressors, and consider the challenges posed regarding unbiased identification of novel DDR-directed strategies in CSCs. A better understanding of the DDR mediating chemo/radioresistance mechanisms in CSCs could lead to novel therapeutic approaches, thereby enhancing treatment efficacy in cancer patients.
Recent Advances in the Systemic Treatment of Localized Gastroesophageal Cancer
Authors: Puhr Hannah Christina , Reiter Thorsten J. , Preusser Matthias , Prager Gerald W. , Ilhan-Mutlu Aysegül
Published: March 2023

The overall survival expectancy of localized gastroesophageal cancer patients still remains under 5 years despite advances in neoadjuvant and adjuvant treatment strategies in recent years. For almost a decade, immunotherapy has been successfully implemented as a first-line treatment for various oncological diseases in advanced stages. In the case of advanced gastroesophageal cancer, 2021 witnessed several approvals of immune checkpoint inhibitor therapies by different authorities. Although it is still a debate whether this treatment should be restricted to a certain subgroup of patients based on biomarker selection, immunotherapy agents are making remarkable steps in resectable settings as well. The Checkmate-577 study demonstrated significant benefits of nivolumab as an adjuvant treatment for resectable esophageal and gastroesophageal junction tumors and thereby obtained approvals both from U.S. American and European authorities. First results of further potential practice-changing clinical trials are expected in 2023, which might change the treatment armamentarium for resectable gastroesophageal cancers significantly. This review aims to demonstrate the advances of immunotherapy and targeted therapies in treatment of localized gastric, gastroesophageal junction and esophageal tumors and gives a short summary on promising ongoing clinical trials.
FoxP3 Expression in Tumor-Infiltrating Lymphocytes as Potential Predictor of Response to Immune Checkpoint Inhibitors in Patients with Advanced Melanoma and Non-Small Cell Lung Cancer
Authors: Grell Peter , Borilova Simona , Fabian Pavel , Selingerova Iveta , Novak David , Muller Petr , Kiss Igor , Vyzula Rostislav
Published: March 2023

Immune checkpoint inhibitors (ICI) are the main therapy currently used in advanced malignant melanoma (MM) and non-small cell lung cancer (NSCLC). Despite the wide variety of uses, the possibility of predicting ICI efficacy in these tumor types is scarce. The aim of our study was to find new predictive biomarkers for ICI treatment. We analyzed, by immunohistochemistry, various cell subsets, including CD3+, CD8+, CD68+, CD20+, and FoxP3+ cells, and molecules such as LAG-3, IDO1, and TGFβ. Comprehensive genomic profiles were analyzed. We evaluated 46 patients with advanced MM (31) and NSCLC (15) treated with ICI monotherapy. When analyzing the malignant melanoma group, shorter median progression-free survival (PFS) was found in tumors positive for nuclear FoxP3 in tumor-infiltrating lymphocytes (TILs) (p = 0.048, HR 3.04) and for CD68 expression (p = 0.034, HR 3.2). Longer PFS was achieved in patients with tumors with PD-L1 TPS ≥ 1 (p = 0.005, HR 0.26). In the NSCLC group, only FoxP3 positivity was associated with shorter PFS and OS. We found that FoxP3 negativity was linked with a better response to ICI in both histological groups.
Thai Rat-Tailed Radish Prevents Hepatocarcinogenesis in Rats by Blocking Mutagenicity, Inducing Hepatic Phase II Enzyme, and Decreasing Hepatic Pro-Inflammatory Cytokine Gene Expression
Authors: Pocasap Piman , Weerapreeyakul Natthida , Wongpoomchai Rawiwan
Published: March 2023

Raphanus sativus L. var. caudatus Alef (RS) is an indigenous Thai plant with nutritional and medicinal values such as anticancer activity, but only in vitro. The chemopreventive effects of RS were, therefore, investigated in the initial stage of hepatocarcinogenesis in rats. Diethylnitrosamine (DEN), a carcinogen, was intraperitoneally injected into rats to induce liver cancer. Along with the DEN injection, either aqueous (RS-H2O) or dichloromethane (RS-DCM) extract was administered orally. Immunohistochemistry was used to detect glutathione S-transferase placental (GST-P) positive foci and apoptotic cells in rat livers as indicators of initial-stage carcinogenesis. The underlying mechanisms of chemoprevention were investigated with (a) antimutagenic activity, (b) hepatic phase II enzyme induction, and (c) hepatic pro-inflammatory cytokine gene expression. The results showed that RS-DCM was more potent than RS-H2O in decreasing GST-P positive foci and apoptotic cells induced by DEN. The mechanisms of RS-DCM (phenolics and sulforaphene contents) against liver carcinogenesis (1) block the activity of carcinogens; (2) elevate phase II detoxifying enzymes; and (3) suppress the pro-inflammatory gene expression. RS-H2O (phenolics contents), in contrast, only decreases pro-inflammatory gene expression. In conclusion, the RS extract consisting of phenolics and isothiocyanates exerted significant chemopreventive activity against DEN-induced liver carcinogenesis.
Deep Learning Approach to Classify Cutaneous Melanoma in a Whole Slide Image
Authors: Li Meng , Abe Makoto , Nakano Shigeo , Tsuneki Masayuki
Published: March 2023

Although the histopathological diagnosis of cutaneous melanocytic lesions is fairly accurate and reliable among experienced surgical pathologists, it is not perfect in every case (especially melanoma). Microscopic examination–clinicopathological correlation is the gold standard for the definitive diagnosis of melanoma. Pathologists may encounter diagnostic controversies when melanoma closely mimics Spitz’s nevus or blue nevus, exhibits amelanotic histopathology, or is in situ. It would be beneficial if diagnosing cutaneous melanocytic lesions can be automated by using deep learning, particularly when assisting surgical pathologists with their workloads. In this preliminary study, we investigated the application of deep learning for classifying cutaneous melanoma in whole-slide images (WSIs). We trained models via weakly supervised learning using a dataset of 66 WSIs (33 melanomas and 33 non-melanomas). We evaluated the models on a test set of 90 WSIs (40 melanomas and 50 non-melanomas), achieving ROC–AUC at 0.821 for the WSI level and 0.936 for the tile level by the best model.
Stromal Senescence following Treatment with the CDK4/6 Inhibitor Palbociclib Alters the Lung Metastatic Niche and Increases Metastasis of Drug-Resistant Mammary Cancer Cells
Authors: Gallanis Gregory , Sharif Ghada , Schmidt Marcel , Friedland Benjamin , Battina Rohith , Rahhal Raneen , Davis John , Khan Irfan , Wellstein Anton , Riegel Anna
Published: March 2023

Background: CDK4/6 inhibitors (CDKi) have improved disease control in hormone-receptor-positive, HER2-negative metastatic breast cancer, but most patients develop progressive disease. Methods: We asked whether host stromal senescence after CDK4/6 inhibition affects metastatic seeding and growth of CDKi-resistant mammary cancer cells by using the p16-INK-ATTAC mouse model of inducible senolysis. Results: Palbociclib pretreatment of naïve mice increased lung seeding of CDKi-resistant syngeneic mammary cancer cells, and this effect was reversed by depletion of host senescent cells. RNA sequencing analyses of lungs from non-tumor-bearing p16-INK-ATTAC mice identified that palbociclib downregulates immune-related gene sets and gene expression related to leukocyte migration. Concomitant senolysis reversed a portion of these effects, including pathway-level enrichment of TGF-β- and senescence-related signaling. CIBERSORTx analysis revealed that palbociclib alters intra-lung macrophage/monocyte populations. Notably, lung metastases from palbociclib-pretreated mice revealed senescent endothelial cells. Palbociclib-treated endothelial cells exhibit hallmark senescent features in vitro, upregulate genes involved with the senescence-associated secretory phenotype, leukocyte migration, and TGF-β-mediated paracrine senescence and induce tumor cell migration and monocyte trans-endothelial invasion in co-culture. Conclusions: These studies shed light on how stromal senescence induced by palbociclib affects lung metastasis, and they describe palbociclib-induced gene expression changes in the normal lung and endothelial cell models that correlate with changes in the tumor microenvironment in the lung metastatic niche.
Areas of Crush Nuclear Streaming Should Be Included as Tumor Content in the Era of Molecular Diagnostics
Authors: Noda Yuri , Yamaka Ryosuke , Atsumi Naho , Higasa Koichiro , Tsuta Koji
Published: March 2023

Degenerated tissues are frequently observed in malignant tumors, but are not analyzed. We investigated whether nuclear streaming and necrosis samples could be used for genetic analysis to expand the sample pool. A total of 81 samples were extracted from small cell carcinoma and lymphoma FFPE tissue blocks and classified into three histological cohorts: 33 materials with well-preserved tumor morphology, 31 nuclear streaming samples, and 17 necrosis samples. DNA and RNA integrity numbers, percentage of RNA fragments with >200 nucleotides, and next-generation sequencing quality metrics were compared among the cohorts. DNA quality did not significantly differ between nuclear streaming materials and materials with well-preserved morphology, whereas that of the necrosis samples was inferior. RNA quality decreased in the following order: materials with well-preserved morphology > nuclear streaming > necrosis. The sequencing metrics did not differ significantly between the nuclear streaming samples and materials with well-preserved morphology, and reliable variants were detected. The necrosis samples extracted from resections exhibited sequencing failure and showed significantly fewer on-target aligned reads and variants. However, variant allele frequency did not differ among the cohorts. We revelated that DNA in nuclear streaming samples, especially within biopsies, could be used for genetic analysis. Moreover, degenerated non-tumor cells should be counted when evaluating tumor content to avoid misinterpreting the variant allele frequency.
Doped Ruthenium/Hypercrosslinked Polystyrene (HPS) Catalysts in the Modification of Fatty Acid Methyl Esters
Authors: Dutra Romulo C. , Martins Thatiane V. S. , Rocha Delma da G. , Meneghetti Mario R. , Meneghetti Simoni M. P. , Sulman Mikhail G. , Matveeva Valentina G. , Suarez Paulo A. Z.
Published: March 2023

The modification of fatty substrates using heterogeneous catalysts have extensive industrial application and has an emphatic position in a sustainable context. Herein ruthenium, nickel, cobalt and copper-doped ruthenium, supported on hypercrosslinked polystyrene (HPS) catalysts were prepared by wet impregnation, characterized and applied on the modification of canola fatty acid methyl esters (FAME). The characterization showed a dispersive effect of doping metals over the ruthenium particles, the presence of acid sites and overall surface morphology, which allows targeting potential applications. The chosen modifications consisted of hydrogenation, hydroformylation, oxidative cleavage and deoxygenation, resulting in superb catalytic activities of over 99% conversions for hydrogenation and deoxygenation. The simplicity of the canola FAME composition allows the understanding of the catalytic processes and allows the upscale of more complex FAME matrixes. Finally, the obtained data stimulates further optimization studies for each application with a variation on the catalysts and the usage of residual fatty substrates, greatly enhancing the sustainability profile of the systems.
Brain Vascular Health in ALS Is Mediated through Motor Cortex Microvascular Integrity
Authors: Schreiber Stefanie , Bernal Jose , Arndt Philipp , Schreiber Frank , Müller Patrick , Morton Lorena , Braun-Dullaeus Rüdiger Christian , Valdés-Hernández Maria Del Carmen , Duarte Roberto , Wardlaw Joanna Marguerite , Meuth Sven Günther , Mietzner Grazia , Vielhaber Stefan , Dunay Ildiko Rita , Dityatev Alexander , Jandke Solveig , Mattern Hendrik
Published: March 2023

Brain vascular health appears to be critical for preventing the development of amyotrophic lateral sclerosis (ALS) and slowing its progression. ALS patients often demonstrate cardiovascular risk factors and commonly suffer from cerebrovascular disease, with evidence of pathological alterations in their small cerebral blood vessels. Impaired vascular brain health has detrimental effects on motor neurons: vascular endothelial growth factor levels are lowered in ALS, which can compromise endothelial cell formation and the integrity of the blood–brain barrier. Increased turnover of neurovascular unit cells precedes their senescence, which, together with pericyte alterations, further fosters the failure of toxic metabolite removal. We here provide a comprehensive overview of the pathogenesis of impaired brain vascular health in ALS and how novel magnetic resonance imaging techniques can aid its detection. In particular, we discuss vascular patterns of blood supply to the motor cortex with the number of branches from the anterior and middle cerebral arteries acting as a novel marker of resistance and resilience against downstream effects of vascular risk and events in ALS. We outline how certain interventions adapted to patient needs and capabilities have the potential to mechanistically target the brain microvasculature towards favorable motor cortex blood supply patterns. Through this strategy, we aim to guide novel approaches to ALS management and a better understanding of ALS pathophysiology.
MicroRNA-223 Dampens Pulmonary Inflammation during Pneumococcal Pneumonia
Authors: Goekeri Cengiz , Pennitz Peter , Groenewald Wibke , Behrendt Ulrike , Kirsten Holger , Zobel Christian M. , Berger Sarah , Heinz Gitta A. , Mashreghi Mir-Farzin , Wienhold Sandra-Maria , Dietert Kristina , Dorhoi Anca , Gruber Achim D. , Scholz Markus , Rohde Gernot , Suttorp Norbert ,   CAPNETZ Study Group, Witzenrath Martin , Nouailles Geraldine
Published: March 2023

Community-acquired pneumonia remains a major contributor to global communicable disease-mediated mortality. Neutrophils play a leading role in trying to contain bacterial lung infection, but they also drive detrimental pulmonary inflammation, when dysregulated. Here we aimed at understanding the role of microRNA-223 in orchestrating pulmonary inflammation during pneumococcal pneumonia. Serum microRNA-223 was measured in patients with pneumococcal pneumonia and in healthy subjects. Pulmonary inflammation in wild-type and microRNA-223-knockout mice was assessed in terms of disease course, histopathology, cellular recruitment and evaluation of inflammatory protein and gene signatures following pneumococcal infection. Low levels of serum microRNA-223 correlated with increased disease severity in pneumococcal pneumonia patients. Prolonged neutrophilic influx into the lungs and alveolar spaces was detected in pneumococci-infected microRNA-223-knockout mice, possibly accounting for aggravated histopathology and acute lung injury. Expression of microRNA-223 in wild-type mice was induced by pneumococcal infection in a time-dependent manner in whole lungs and lung neutrophils. Single-cell transcriptome analyses of murine lungs revealed a unique profile of antimicrobial and cellular maturation genes that are dysregulated in neutrophils lacking microRNA-223. Taken together, low levels of microRNA-223 in human pneumonia patient serum were associated with increased disease severity, whilst its absence provoked dysregulation of the neutrophil transcriptome in murine pneumococcal pneumonia.
Reduced Levels of Misfolded and Aggregated Mutant p53 by Proteostatic Activation
Authors: Naus Evelyne , Derweduwe Marleen , Lampi Youlia , Claeys Annelies , Pauwels Jarne , Langenberg Tobias , Claes Filip , Xu Jie , Haemels Veerle , Atak Zeynep Kalender , van der Kant Rob , Van Durme Joost , De Baets Greet , Ligon Keith L. , Fiers Mark , Gevaert Kris , Aerts Stein , Rousseau Frederic , Schymkowitz Joost , De Smet Frederik
Published: March 2023

In malignant cancer, excessive amounts of mutant p53 often lead to its aggregation, a feature that was recently identified as druggable. Here, we describe that induction of a heat shock-related stress response mediated by Foldlin, a small-molecule tool compound, reduces the protein levels of misfolded/aggregated mutant p53, while contact mutants or wild-type p53 remain largely unaffected. Foldlin also prevented the formation of stress-induced p53 nuclear inclusion bodies. Despite our inability to identify a specific molecular target, Foldlin also reduced protein levels of aggregating SOD1 variants. Finally, by screening a library of 778 FDA-approved compounds for their ability to reduce misfolded mutant p53, we identified the proteasome inhibitor Bortezomib with similar cellular effects as Foldlin. Overall, the induction of a cellular heat shock response seems to be an effective strategy to deal with pathological protein aggregation. It remains to be seen however, how this strategy can be translated to a clinical setting.
Social Isolation Activates Dormant Mammary Tumors, and Modifies Inflammatory and Mitochondrial Metabolic Pathways in the Rat Mammary Gland
Authors: Andrade Fabia de Oliveira , Jin Lu , Clarke Robert , Wood Imani , Dutton MaryAnn , Anjorin Chezaray , Rubin Grace , Gao Audrey , Sengupta Surojeet , FitzGerald Kevin , Hilakivi-Clarke Leena
Published: March 2023

Although multifactorial in origin, one of the most impactful consequences of social isolation is an increase in breast cancer mortality. How this happens is unknown, but many studies have shown that social isolation increases circulating inflammatory cytokines and impairs mitochondrial metabolism. Using a preclinical Sprague Dawley rat model of estrogen receptor-positive breast cancer, we investigated whether social isolation impairs the response to tamoxifen therapy and increases the risk of tumors emerging from dormancy, and thus their recurrence. We also studied which signaling pathways in the mammary glands may be affected by social isolation in tamoxifen treated rats, and whether an anti-inflammatory herbal mixture blocks the effects of social isolation. Social isolation increased the risk of dormant mammary tumor recurrence after tamoxifen therapy. The elevated recurrence risk was associated with changes in multiple signaling pathways including an upregulation of IL6/JAK/STAT3 signaling in the mammary glands and tumors and suppression of the mitochondrial oxidative phosphorylation (OXPHOS) pathway. In addition, social isolation increased the expression of receptor for advanced glycation end-products (RAGE), consistent with impaired insulin sensitivity and weight gain linked to social isolation. In socially isolated animals, the herbal product inhibited IL6/JAK/STAT3 signaling, upregulated OXPHOS signaling, suppressed the expression of RAGE ligands S100a8 and S100a9, and prevented the increase in recurrence of dormant mammary tumors. Increased breast cancer mortality among socially isolated survivors may be most effectively prevented by focusing on the period following the completion of hormone therapy using interventions that simultaneously target several different pathways including inflammatory and mitochondrial metabolism pathways.
Chromatin Accessibility and Transcriptional Landscape during Inhibition of Salmonella enterica by Lactobacillus reuteri in IPEC-J2 Cells
Authors: Qin Weiyun , Ren Zhanshi , Xu Chao , Cao Ya-nan , Sun Ming-an , Huang Ruihua , Bao Wenbin
Published: March 2023

Lactobacillus reuteri is a probiotic with bacteriostatic effects, which can effectively inhibit the activity of pathogens. However, the molecular mechanism underlying the inhibition of pathogens by L. reuteri in intestinal cells remains unclear. Using the porcine intestinal cell line IPEC-J2 as a model, we combined RNA-seq and ATAC-seq methods to delineate the porcine genome-wide changes in biological processes and chromatin accessibility in IPEC-J2 cells stimulated by Salmonella enterica BNCC186354, as well as L. reuteri ATCC 53608. Overall, we found that many porcine transcripts were altered after S. enterica BNCC186354 treatment, while L. reuteri ATCC 53608 treatment partially restored this alteration, such as salmonella infection and PI3K/AKT and MAPK pathways. Combined analysis of these two datasets revealed that 26 genes with similar trends overlapped between gene expression and chromatin accessibility. In addition, we identified potential host functional transcription factors (TFs), such as GATA1, TAL1, TBP, RUNX1, Gmeb1, Gfi1b, RARA, and RXRG, in IPEC-J2 cells that might play a critical role and are targeted by L. reuteri ATCC 53608. Moreover, we verified that PI3K/AKT, MAPK, and apoptosis pathways are potentially regulated by S. enterica BNCC186354 but restored by L. reuteri ATCC 53608. The PI3K/AKT pathway was activated by L. reuteri ATCC 53608, thereby potentially inhibiting S. enterica BNCC186354 infection. In conclusion, our data provide new insights into the expression pattern of functional genes and the epigenetic alterations in IPEC-J2 cells underlying the bacteriostatic action of L. reuteri ATCC 53608.
Emotional Regulation Interventions on Developmental Course for Preterm Children: A Systematic Review of Randomized Control Trials
Authors: Dell’Aversana Vincenza , Tofani Marco , Valente Donatella
Published: March 2023

Children born preterm (<37 weeks of gestation) are at increased risk of socio-emotional difficulties. This study aims to determine the effects of rehabilitation intervention on the emotional regulation of children born preterm through a systematic review. We conducted a systematic review according to PRISMA guidelines. The literature screening was carried out on PUBMED, SCOPUS and WEB OF SCIENCE in August 2022. An author identified eligible studies based on predefined inclusion criteria and extracted the data. RCT quality was assessed using the JADAD and PEDro scales. We selected five RCTs for qualitative synthesis, having the common objective of evaluating the changes in emotional regulation after a rehabilitation intervention. Evidence of benefits was found after parent training intervention (PCIT; p < 0.05). Moreover, there was an improvement in day-to-day executive life and fewer behavioral problems after mindfulness intervention. Clinical, but not statistical, efficacy was found for the group-based physiotherapy intervention. In conclusion, parent training and mindfulness interventions can be helpful rehabilitation techniques, but the relatively small sample limited statistical power, so the discovery needs to be interpreted cautiously. Further research on these aspects is recommended.
Determinants of Health Inequalities in Iran and Saudi Arabia: A Systematic Review of the Sleep Literature
Authors: Ndiaye Comsar , Ayedi Yosr , Etindele Sosso Faustin Armel
Published: March 2023

Sleep health inequalities represent an increasing public health concern. Among multiple determinants affecting sleep health, there is people’s socioeconomic status (SES), and no systematic review on the relationship between SES and sleep health has been previously conducted in Iran and Saudi Arabia. Following the Prisma protocol, ten articles were selected. Findings revealed that the combined number of participants was N = 37,455 participants, including 73.23% of children and adolescents (n = 27,670) and 26.77% of adults (n = 10,026). The smallest sample was N = 715 and the larger was N = 13,486. In all these studies, sleep variables were assessed using self-reported questionnaires. The studies conducted in Iran assessed the risk of obstructive sleep apnea (OSA), while those in Saudi Arabia were interested in sleep duration, nap time, bedtime, rise time and insomnia. The studies performed on adult populations in Iran and Saudi Arabia concluded that there is no significant association between SES determinants and sleep components in adult populations. One study in Iran found a significant association between parent’s low SES and children and adolescent insomnia; and one study in Saudi Arabia found a significant association between the father’s education and the longer sleep duration of their children. More longitudinal studies are necessary to establish a causal relationship between public health policies and sleep health inequalities. An extension of the investigation to more sleep disturbances is required to cover the entirety of sleep health inequalities in Iran and Saudi Arabia.
Decomposition of Fingerprints on Porous TiO2 Thin Films
Authors: Lee Minsu , Kim Hohyeong , Kim Hyungsub , Katoch Akash , Park Jae Young
Published: March 2023

This study investigated the effect of the mixing ratio of TiO2 nanoparticles (P25) and titanium alkoxide (T-sol) on various properties of TiO2 films. The specific surface area of the TiO2 film was determined using BET analysis, while the microstructure and thickness were analyzed by field emission scanning electron microscopy (FE-SEM) and atomic force microscopy (AFM), respectively. Transmittance and pencil hardness tests were conducted to evaluate the transparency and durability of the coating layer, respectively. The results showed that, as the P25 content increased, the specific surface area of the TiO2 film also increased, but this effect decreased as the ratio of T-sol to P25 increased. Additionally, the thickness and surface roughness (Ra) of the coating layer increased as the P25 content increased, with the thickness increasing from 210 to 950 nm and Ra increasing from 51 to 88 nm. However, the transmittance of the coating layer decreased as the P25 content increased, indicating that the films became less transparent. Furthermore, the pencil hardness of the coating layer decreased as the P25 content increased, indicating that the films became less durable. Finally, the oil contact angle decreased as the P25 content increased, indicating that the films became more hydrophilic.
The Effect of the COVID-19 Pandemic on Unrelated Allogeneic Hematopoietic Donor Collections and Safety
Authors: Parmar Gaganvir , Allan David S. , Morris Gail , Dibdin Nicholas , Ganz Kathy , Mostert Karen , Paulson Kristjan , Petraszko Tanya , Stevens Nora , Seftel Matthew D.
Published: March 2023

Background and Objectives: The COVID-19 pandemic profoundly influenced unrelated donor (UD) allogeneic peripheral blood stem cell (PBSC) collections. Changes included efforts to minimize COVID-19 exposure to donors and cryopreservation of products. The extent to which the efficacy and safety of PBSC donations were affected by the pandemic is unknown. Methods: Prospective cohort analysis of PBSC collections comparing pre-pandemic (01 April 2019–14 March 2020) and pandemic (15 March 2020–31 March 2022) eras. Results: Of a total of 291 PBSC collections, cryopreservation was undertaken in 71.4% of pandemic donations compared to 1.1% pre-pandemic. The mean requested CD34+ cell dose/kg increased from 4.9 ± 0.2 × 106 pre-pandemic to 5.4 ± 0.1 × 106 during the pandemic. Despite this increased demand, the proportion of collections that met or exceeded the requested cell dose did not change, and the mean CD34+ cell doses collected (8.9 ± 0.5 × 106 pre-pandemic vs. 9.7 ± 0.4 × 106 during the pandemic) remained above requested targets. Central-line placements were more frequent, and severe adverse events in donors increased during the pandemic. Conclusion: Cryopreservation of UD PBSC products increased during the pandemic. In association with this, requested cell doses for PBSC collections increased. Collection targets were met or exceeded at the same frequency, signaling high donor and collection center commitment. This was at the expense of increased donor or product-related severe adverse events. We highlight the need for heightened vigilance about donor safety as demands on donors have increased since the pandemic.
Alcoholic Liver Disease-Related Hepatocellular Carcinoma: Characteristics and Comparison to General Slovak Hepatocellular Cancer Population
Authors: Šafčák Dominik , Dražilová Sylvia , Gazda Jakub , Andrašina Igor , Adamcová-Selčanová Svetlana , Barila Radovan , Mego Michal , Rác Marek , Skladaný Ľubomír , Žigrai Miroslav , Janičko Martin , Jarčuška Peter
Published: March 2023

Hepatocellular carcinoma (HCC) has multiple molecular classes that are associated with distinct etiologies and, besides particular molecular characteristics, that also differ in clinical aspects. We aim to characterize the clinical aspects of alcoholic liver disease-related HCC by a retrospective observational study that included all consequent patients diagnosed with MRI or histologically verified HCC in participating centers from 2010 to 2016. A total of 429 patients were included in the analysis, of which 412 patients (96%) had cirrhosis at the time of diagnosis. The most common etiologies were alcoholic liver disease (ALD) (48.3%), chronic hepatitis C (14.9%), NAFLD (12.6%), and chronic hepatitis B (10%). Patients with ALD-related HCC were more commonly males, more commonly had cirrhosis that was in more advanced stages, and had poorer performance status. Despite these results, no differences were observed in the overall (median 8.1 vs. 8.5 months) and progression-free survival (median 4.9 vs. 5.7 months). ALD-HCC patients within BCLC stage 0–A less frequently received potentially curative treatment as compared to the control HCC patients (62.2% vs. 87.5%, p = 0.017); and in patients with ALD-HCC liver function (MELD score) seemed to have a stronger influence on the prognosis compared to the control group HCC. Systemic inflammatory indexes were strongly associated with survival in the whole cohort. In conclusion, alcoholic liver disease is the most common cause of hepatocellular carcinoma in Slovakia, accounting for almost 50% of cases; and patients with ALD-related HCC more commonly had cirrhosis that was in more advanced stages and had poorer performance status, although no difference in survival between ALD-related and other etiology-related HCC was observed.
Aliens and Returnees: Review of Neobiotic Species of Freshwater Mollusks in Siberia from the Kazakhstan Steppe to the Arctic Tundra
Authors: Babushkin Evgeny S. , Nekhaev Ivan O. , Vinarski Maxim V. , Yanygina Liubov V.
Published: March 2023

This article reviews all available information on the species composition, current distribution, and origins of the neobiotic (non-indigenous and restoring the lost range) freshwater mollusks in Siberia. An extensive literary search has been carried out, and virtually all existing publications of recent decades on the findings of freshwater mollusk species new to Siberia were taken into account. We examined extensive malacological collections of some of Russia’s and Kazakhstan’s scientific organizations. The core of the examined material is our own observations and collections made in various parts of Siberia and adjacent areas. An annotated checklist of neobiotic species of mollusks reliably recorded in Siberia is presented, and probable mechanisms and “corridors” of infiltration of these species into the region are discussed. Most of the discovered snail species belong to a group popular among aquarists, and their source of introduction is obvious. Another large portion of species infiltrate into the region with the development of fish farming. A classification of species of neobiotic freshwater mollusks of Siberia was proposed and a forecast was made for changes in the Siberian freshwater malacofauna for the coming decades. In our opinion, at present it is possible to accept the newest stage in the genesis of the freshwater malacofauna of Siberia, occurring in conditions specific to the Anthropocene.
Seasonality and Long-Term Effect of Environmental Variables on the Orb Weaver Spider Community of a Tropical Dry Forest in the Balsas Basin, Mexico
Authors: Menéndez-Acuña Miguel , Salas-Rodríguez Mónica , Montiel-Parra Griselda , Sotuyo Solange , Rosas-Echeverría Ma Ventura
Published: March 2023

Spiders are significant predators in terrestrial habitats, with the highest diversity found in tropical ecosystems, but it is also where they are studied least. In this study, we analyzed the effect of seasonality and three environmental variables (precipitation, evaporation, and temperature) in the orb-weaver composition, richness, abundance, dominance, and diversity. In addition, the species turnover between three localities with differences in the vegetation composition and maintenance in the Sierra de Huautla, Morelos, Mexico, were evaluated. The collection methods used included beating vegetation and direct searches conducted over seven hours during daylight, once per month, between April 2013 and March 2014. A total of 17 species from 14 genera were registered. With the records, we compiled the first species inventory of araneids for the three localities, adding eight new species records for the state of Morelos. The predominant species were Neoscona oaxacensis (Keyserling, 1864) and Trichonephila clavipes (Linnaeus, 1767), but over 50% of the species that we recorded corresponded to a single individual. The Araneid community changed between the dry and rainy seasons in all three localities, with each locality responding differently to changes in environmental variables, with most variables maintaining a long-term effect on the spider community.
Galactomannan-Antigen Testing from Non-Directed Bronchial Lavage for Rapid Detection of Invasive Pulmonary Aspergillosis in Critically Ill Patients: A Proof-of-Concept Study
Authors: Rothe Kathrin , Dibos Miriam , Haschka Stefanie J. , Schmid Roland M. , Busch Dirk , Rasch Sebastian , Lahmer Tobias
Published: March 2023

Invasive pulmonary aspergillosis is associated with high mortality. For diagnosis, galactomannan-antigen in serum and bronchoalveolar lavage fluid is recommended, with higher sensitivity in bronchoalveolar lavage fluid. Because of invasiveness, bronchoalveolar lavage might be withheld due to patients’ or technical limitations, leading to a delay in diagnosis while early diagnosis is crucial for patient outcome. To address this problem, we performed an analysis of patient characteristics of intubated patients with invasive pulmonary aspergillosis with comparison of galactomannan-antigen testing between non-directed bronchial lavage (NBL) and bronchoalveolar lavage fluid. A total of 32 intubated ICU patients with suspected invasive pulmonary aspergillosis could be identified. Mycological cultures were positive in 37.5% for A. fumigatus. Galactomannan-antigen in NBL (ODI 4.3 ± 2.4) and bronchoalveolar lavage fluid (ODI 3.6 ± 2.2) showed consistent results (p-value 0.697). Galactomannan-antigen testing for detection of invasive pulmonary aspergillosis using deep tracheal secretion showed comparable results to bronchoalveolar lavage fluid. Because of widespread availability in intubated patients, galactomannan-antigen from NBL can be used as a screening parameter in critical risk groups with high pretest probability for invasive aspergillosis to accelerate diagnosis and initiation of treatment. Bronchoalveolar lavage remains the gold standard for diagnosis of invasive aspergillosis to be completed to confirm diagnosis, but results from NBL remove time sensitivity.
Menopausal Changes in the Microbiome—A Review Focused on the Genitourinary Microbiome
Authors: Park Min Gu , Cho Seok , Oh Mi Mi
Published: March 2023

A balanced interaction between the host and its microbiome is crucial to health. Research regarding the significance of the gut and vaginal microbiomes in female health is substantial. However, less data regarding the urinary microbiome are available. Interactions between the gut, vaginal, and urinary microbiomes are also currently being researched. Hormone-induced dysbiosis after menopause is believed to have effects on physical changes and health consequences. Postmenopausal changes in the gut microbiome are associated with increased short-chain fatty acids and hydrogen sulfide levels. Increased vaginal pH caused by reduced estrogen alters the vaginal microbiome, resulting in reduced levels of Lactobacillus. Such changes influence the vaginal structure and functions, contributing to the onset of genitourinary syndrome of menopause. A dysbiosis of the urinary microbiome is associated with urgency and urinary incontinence and also related to interstitial cystitis/bladder pain syndrome and neuropathic bladder. As these diseases commonly affect postmenopausal women, hormone-induced changes in the microbiome may play a role. Menopause increases the alpha diversity of the urinary microbiome and lowers the percentage of Lactobacillus in urine, and such changes precede recurrent cystitis. More research regarding the effects of changes in the urinary microbiome due to menopause on urinary tract diseases is needed.
Rare Benign Tumors and Tumor-Like Lesions of the Hand without Skin Damage—Clinical, Imagistic and Histopathological Diagnosis, Retrospective Study
Authors: Pertea Mihaela , Grosu Oxana Madalina , Filip Alexandru , Moraru Dan Cristian , Luca Stefana , Fotea Madalina-Cristina , Lunca Sorinel , Olinici Doinita , Poroch Vladimir , Carp Claudiu , Veliceasa Bogdan
Published: March 2023

Background: The broad spectrum of diagnoses and clinical features of hand tumors and the absence of pathognomonic signs often lead to an inaccurate or delayed diagnosis. However, only a few reports have comprehensively referenced the diagnosis and clinical features of hand tumors. This study intends to highlight the clinical, imaging and histological characteristics of uncommon hand tumors or tumor-like lesions. Methods: In this retrospective study, we report a series of 80 patients diagnosed with rare hand tumors and tumor-like lesions without skin damage. Age, gender, tumor location, imaging examinations and clinical and laboratory findings were analyzed. The histopathological exam established the final diagnosis. Surgery was indicated and performed in all cases. Results: This study included: neurofibroma, glomus tumor, lipoma, schwannoma, epidermal inclusion cyst and idiopathic tenosynovitis with “rice bodies.” We have described the clinical, imagistic and histopathological particularities of these tumors. Surgical management included the complete removal of tumors, with no recurrence recorded within two years and overall high patient satisfaction. The most common findings were lipomas and the rarest neurofibromas. Conclusions: To optimize the care of hand tumors and reduce diagnostic and treatment errors, knowledge of hand tumor types and their clinical and laboratory characteristics is necessary for every surgeon.
NaRnEA: An Information Theoretic Framework for Gene Set Analysis
Authors: Griffin Aaron T. , Vlahos Lukas J. , Chiuzan Codruta , Califano Andrea
Published: March 2023

Gene sets are being increasingly leveraged to make high-level biological inferences from transcriptomic data; however, existing gene set analysis methods rely on overly conservative, heuristic approaches for quantifying the statistical significance of gene set enrichment. We created Nonparametric analytical-Rank-based Enrichment Analysis (NaRnEA) to facilitate accurate and robust gene set analysis with an optimal null model derived using the information theoretic Principle of Maximum Entropy. By measuring the differential activity of ~2500 transcriptional regulatory proteins based on the differential expression of each protein’s transcriptional targets between primary tumors and normal tissue samples in three cohorts from The Cancer Genome Atlas (TCGA), we demonstrate that NaRnEA critically improves in two widely used gene set analysis methods: Gene Set Enrichment Analysis (GSEA) and analytical-Rank-based Enrichment Analysis (aREA). We show that the NaRnEA-inferred differential protein activity is significantly correlated with differential protein abundance inferred from independent, phenotype-matched mass spectrometry data in the Clinical Proteomic Tumor Analysis Consortium (CPTAC), confirming the statistical and biological accuracy of our approach. Additionally, our analysis crucially demonstrates that the sample-shuffling empirical null models leveraged by GSEA and aREA for gene set analysis are overly conservative, a shortcoming that is avoided by the newly developed Maximum Entropy analytical null model employed by NaRnEA.
Energy Dispatch for CCHP System in Summer Based on Deep Reinforcement Learning
Authors: Gao Wenzhong , Lin Yifan
Published: March 2023

Combined cooling, heating, and power (CCHP) system is an effective solution to solve energy and environmental problems. However, due to the demand-side load uncertainty, load-prediction error, environmental change, and demand charge, the energy dispatch optimization of the CCHP system is definitely a tough challenge. In view of this, this paper proposes a dispatch method based on the deep reinforcement learning (DRL) algorithm, DoubleDQN, to generate an optimal dispatch strategy for the CCHP system in the summer. By integrating DRL, this method does not require any prediction information, and can adapt to the load uncertainty. The simulation result shows that compared with strategies based on benchmark policies and DQN, the proposed dispatch strategy not only well preserves the thermal comfort, but also reduces the total intra-month cost by 0.13~31.32%, of which the demand charge is reduced by 2.19~46.57%. In addition, this method is proven to have the potential to be applied in the real world by testing under extended scenarios.
Analysis of the Energy Loss and Performance Characteristics in a Centrifugal Pump Based on Sinusoidal Tubercle Volute Tongue
Authors: Lin Peifeng , Wang Chunhe , Song Pengfei , Li Xiaojun
Published: March 2023

The energy loss inside a centrifugal pump has a significant effect on its performance characteristics. Based on the structural characteristics of the humpback pectoral fin, a new tongue was designed to improve the performance of the centrifugal pump. The influence of three sinusoidal tubercle volute tongues (STVT) and one original volute tongue (OVT) on energy dissipation using the enstrophy analysis method was investigated. To accomplish this, the pressure fluctuations and performances of four centrifugal pumps were analyzed. The results indicate that enstrophy is primarily distributed at the impeller outlet and near the tongue. The total enstrophy of the profiles of STVT was smaller than that of the profiles of OVT. This difference was more obvious near the tongue. The reductions in the total enstrophy of the pumps were 8% (STVT−1), 8.2% (STVT−2), and 9% (STVT−3). The pressure fluctuations of the STVT profiles also decreased to different degrees. The average pressure fluctuations at the monitoring points decreased by 20.6% (STVT−1), 21.7% (STVT−2), and 23.3% (STVT−3). The performances of the bionic retrofit pumps increased by 1.5% (STVT−1), 2% (STVT−2), and 2.45% (STVT−3) under the design flow rate. This study guides the structural optimization of pumps.
Using Robotics in the Learning of Computer Programming: Student Experiences Based on Experiential Learning Cycles
Authors: Govender Reginald Gerald , Govender Desmond Wesley
Published: March 2023

The understanding of basic constructs in computer programming has always been seen by students as challenging, especially for novice programmers with no prior exposure at the school level. This paper emanates from a larger study and sets out to explore the use of robotics to enhance the learning of computer programming by asking: what are students’ experiences of using robotics when learning to program? Guided by Kolb’s Experiential Learning Cycle, a series of workshops were conducted that promotes hands-on learning and encourages the use of tools in the learning process for knowledge development. The site for this study was a university campus in KwaZulu-Natal, South Africa. The sample was composed of 75 students, most of whom were first-year students who had just started a computer course and had no prior exposure to Computer Programming. The findings showed that the meaningful adoption of Kolb’s experiential learning has proven to be successful in the progressional development of computer programming constructs when using a physical component such as a robot. The use of microcontrollers that provide a robotic element offering a physical attribute during the learning of code proves to be affective. It is hoped that the findings of this study will contribute to the development of innovative methods to introduce computer programming through the use of robotics.