bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigations of Major α-Dicarbonyl Content in U.S. Honey of Different Geographical Origins
Authors: Nyarko Kate , Greenlief C. Michael
Published: April 2024

α-Dicarbonyls are significant degradation products resulting from the Maillard reaction during food processing. Their presence in foods can indicate the extent of heat exposure, processing treatments, and storage conditions. Moreover, they may be useful in providing insights into the potential antibacterial and antioxidant activity of U.S. honey. Despite their importance, the occurrence of α-dicarbonyls in honey produced in the United States has not been extensively studied. This study aims to assess the concentrations of α-dicarbonyls in honey samples from different regions across the United States. The identification and quantification of α-dicarbonyls were conducted using reverse-phase liquid chromatography after derivatization with o-phenylenediamine (OPD) and detected using ultraviolet (UV) and mass spectrometry methods. This study investigated the effects of pH, color, and derivatization reagent on the presence of α-dicarbonyls in honey. The quantification method was validated by estimating the linearity, precision, recovery, method limit of detection, and quantification using known standards for GO, MGO, and 3-DG, respectively. Three major OPD-derivatized α-dicarbonyls including methylglyoxal (MGO), glyoxal (GO), and 3-deoxyglucosone (3-DG), were quantified in all the honey samples. 3-Deoxyglucosone (3-DG) was identified as the predominant α-dicarbonyl in all the U.S. honey samples, with concentrations ranging from 10.80 to 50.24 mg/kg. The total α-dicarbonyl content ranged from 16.81 to 55.74 mg/kg, with the highest concentration measured for Southern California honey. Our results showed no significant correlation between the total α-dicarbonyl content and the measured pH solutions. Similarly, we found that lower amounts of the OPD reagent are optimal for efficient derivatization of MGO, GO, and 3-DG in honey. Our results also indicated that darker types of honey may contain higher α-dicarbonyl content compared with lighter ones. The method validation results yielded excellent recovery rates for 3-DG (82.5%), MGO (75.8%), and GO (67.0%). The method demonstrated high linearity with a limit of detection (LOD) and limit of quantitation (LOQ) ranging from 0.0015 to 0.002 mg/kg and 0.005 to 0.008 mg/kg, respectively. Our results provide insights into the occurrence and concentrations of α-dicarbonyl compounds in U.S. honey varieties, offering valuable information on their quality and susceptibility to thermal processing effects.
Optimization of the Amplification of Equine Muscle-Derived Mesenchymal Stromal Cells in a Hollow-Fiber Bioreactor
Authors: Duysens Julien , Graide Hélène , Niesten Ariane , Mouithys-Mickalad Ange , Ceusters Justine , Serteyn Didier
Published: April 2024

The main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to achieve this rapid and total regeneration, systemic injections of 1 to 2 million cells per kilogram of body weight must be considered. Here, we demonstrate for the first time the feasibility and expansion capacity of equine muscle-derived mesenchymal stromal cells (mdMSCs) in a functionally closed, automated, perfusion-based, hollow-fiber bioreactor (HFBR) called the Quantum™ Cell Expansion System (Terumo Blood and Cell Technologies). This feature greatly increases the number of generated cells with a surface area of 1.7 m2. The expansion of mdMSCs is very efficient in this bioreactor. The maximum expansion generated twenty times more cells than the initial seeding in nine days. The best returns were observed with an optimal seeding between 10 and 25 million mdMSCs, using the Bull’s eye loading method and with a run duration between 7 and 10 days. Moreover, all the generated cells kept their stem properties: the ability to adhere to plastic and to differentiate into chondroblasts, osteoblasts and adipocytes. They also showed the expression of CD-44 and CD-90 markers, with a positive rate above 93%, while CD-45 and MHCII were non-expressed, with a positive rate below 0.5%. By capitalizing on the scalability, automation and 3D culture capabilities of the Quantum™, it is possible to generate large quantities of high-quality equine mdMSCs for gastrointestinal disorders and other clinical applications.
Pre-Diagnosis Diet Predicts Response to Exclusive Enteral Nutrition and Correlates with Microbiome in Pediatric Crohn Disease
Authors: Dijk Stephanie , Jarman Megan , Zhang Zhengxiao , Lawley Morgan , Ahmad Muzammil , Suarez Ricardo , Rossi Laura , Chen Min , Wu Jessica , Carroll Matthew W. , Otley Anthony , Sherlock Mary , Mack David R. , Jacobson Kevan , deBruyn Jennifer C. , El-Matary Wael , Deslandres Colette , Rashid Mohsin , Church Peter C. , Walters Thomas D. , Huynh Hien Q. , Surette Michael G. , Griffiths Anne M. , Wine Eytan
Published: April 2024

Exclusive enteral nutrition (EEN) is effective in inducing remission in pediatric Crohn disease (CD). EEN alters the intestinal microbiome, but precise mechanisms are unknown. We hypothesized that pre-diagnosis diet establishes a baseline gut microbiome, which then mediates response to EEN. We analyzed prospectively recorded food frequency questionnaires (FFQs) for pre-diagnosis dietary patterns. Fecal microbiota were sequenced (16SrRNA) at baseline and through an 18-month follow-up period. Dietary patterns, Mediterranean diet adherence, and stool microbiota were associated with EEN treatment outcomes, disease flare, need for anti-tumor necrosis factor (TNF)-α therapy, and long-term clinical outcomes. Ninety-eight patients were included. Baseline disease severity and microbiota were associated with diet. Four dietary patterns were identified by FFQs; a “mature diet” high in fruits, vegetables, and fish was linked to increased baseline microbial diversity, which was associated with fewer disease flares (p < 0.05) and a trend towards a delayed need for anti-TNF therapy (p = 0.086). Baseline stool microbial taxa were increased (Blautia and Faecalibacterium) or decreased (Ruminococcus gnavus group) with the mature diet compared to other diets. Surprisingly, a “pre-packaged” dietary pattern (rich in processed foods) was associated with delayed flares in males (p < 0.05). Long-term pre-diagnosis diet was associated with outcomes of EEN therapy in pediatric CD; diet–microbiota and microbiota–outcome associations may mediate this relationship.
Anti-Inflammatory, Analgesic, Functional Improvement, and Chondroprotective Effects of Erigeron breviscapus (Vant.) Hand.-Mazz. Extract in Osteoarthritis: An In Vivo and In Vitro Study
Authors: Jo Hee-Geun , Baek Chae Yun , Lee JunI , Hwang Yeseul , Baek Eunhye , Hwang Ji Hye , Lee Donghun
Published: April 2024

Osteoarthritis (OA) is a degenerative bone disease characterized by inflammation as a primary pathology and currently lacks therapeutic interventions to impede its progression. Erigeron breviscapus (Vant.) Hand.-Mazz. (EB) is an east Asian herbal medicine with a long history of use and a wide range of confirmed efficacy against cardiovascular and central nervous system diseases. The purpose of this study is to evaluate whether EB is worthy of further investigation as a treatment for OA based on anti-inflammatory activity. This study aims to assess the potential of EB as a treatment for OA, focusing on its anti-inflammatory properties. Analgesic effects, functional improvements, and inhibition of cartilage destruction induced by EB were evaluated in acetic acid-induced peripheral pain mice and monosodium iodoacetate-induced OA rat models. Additionally, the anti-inflammatory effect of EB was assessed in serum and cartilage tissue in vivo, as well as in lipopolysaccharide-induced RAW 264.7 cells. EB demonstrated a significant alleviation of pain, functional impairment, and cartilage degradation in OA along with a notable inhibition of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, matrix metalloproteinases 13, and nitric oxide synthase 2, both in vitro and in vivo, in a dose-dependent manner compared to the active control. Accordingly, EB merits further exploration as a potential disease-modifying drug for OA, capable of mitigating the multifaceted pathology of osteoarthritis through its anti-inflammatory properties. Nonetheless, additional validation through a broader experimental design is essential to substantiate the findings of this study.
Proanthocyanidins and Phenolic Compounds from the Twigs of Salix chaenomeloides and Their Anti-Lipogenic Effects on 3T3-L1 Preadipocytes
Authors: Kim Kyung Ah , Tran Nguyen Khoi Song , Baek Jiwon , Lee Soah , Kang Ki Sung , Kim Ki Hyun
Published: April 2024

The present study investigated potential bioactive natural products from the EtOH extract of Salix chaenomeloides twigs using column chromatography, leading to the isolation of six compounds (1–6), which were characterized as two proanthocyanidins, procyanidin B2 (1) and procyanidin B1 (2), and four phenolic compounds, 4-hydroxybenzoic acid β-D-glucosyl ester (3), di-O-methylcrenatin (4), p-coumaric acid glucoside (5), and syringin (6) by the comparison of their NMR spectra with the reported data and high-resolution (HR)-electrospray ionization mass spectroscopy (ESI-MS) analysis. We investigated the potential of six compounds (1–6) to inhibit adipogenesis in 3T3-L1 preadipocytes, which showed that the compounds (1–6) significantly reduced lipid accumulation in 3T3-L1 adipocytes without affecting cell proliferation. Notably, compound 1 demonstrated a remarkable 60% and 90% reduction in lipid levels with 50 and 100 µM treatments, respectively. Oil Red O staining results indicated that compound 1 significantly inhibits the formation of lipid droplets, comparable to the effect of T863, an inhibitor of triglyceride used as a positive control, in adipocytes. Compound 1 had no effect on the regulators PPARγ, C/EBPα, and SREBF1 of adipocyte differentiation in 3T3-L1 preadipocytes, but compound 1 activated the fatty acid oxidation regulator, PPARα, compared to the lipogenic-induced control. It also suppressed fatty acid synthesis by downregulating the expression of fatty acid synthase (FAS). Finally, compound 1 induced the mRNA and protein levels of CPT1A, an initial marker of mitochondrial fatty acid oxidation in 3T3-L1. This finding substantiates the anti-lipogenic and lipolytic effects of procyanidin B2 (1) in 3T3-L1 preadipocytes, emphasizing its pivotal role in modulating obesity-related markers.
Knowledge about Fertility in Croatia, Measured with the Croatian Version of the Cardiff Fertility Knowledge Scale (CFKS-Hr), in Relation to Attitudes toward Having a Child and Associated Factors in a Cross-Sectional Survey
Authors: Buljubašić Ante , Švaljug Deana , Mudnić Pulje Martina , Gusar Ivana , Jerković Jelena , Jerončić Tomić Iris
Published: April 2024

Background: Fertility is a crucial component of population maintenance and growth. A declining fertility trend has been observed over the past two decades, and it continues. The birth rate in the Republic of Croatia is continuously decreasing, which is insufficient to maintain its population. Objective: This research aimed to examine the level of fertility knowledge, factors influencing fertility knowledge, and the relationship between fertility knowledge and the decision to have a child. Methods: A cross-sectional study was conducted on a sample of mothers in five hospitals on the Adriatic coast of the Republic of Croatia, involving 1541 mothers, 18 years and older, from September 2021 to December 2023. The Cardiff Fertility Knowledge Scale (CFKS) was used for the research. The participants were divided into four groups according to age. The analysis was conducted using SPSS software (version 22.0). Descriptive statistics, chi-squared tests, t-tests, and Pearson’s correlations were used for the data analysis. Results: The average percent correct score on the CFKS was 51.8% (SD 22.6), with greater knowledge being significantly related to married status and higher education status (both p < 0.05). A total of 83% of mothers who have one child want to have more children. Fewer mothers who have two children want to have more children (42%), while only 11% of mothers who have three or more children want to have more children. There is no significant relationship between the CFKS and the importance of childbearing in the future (p = 0.12). Respondents indicated that they gained most of their knowledge from the internet (31%) and from the healthcare system (33%). Conclusions: The research results reveal a lack of fertility knowledge among participants, as well as an intention to have a child in the later stages of life. The lack of formal education on this topic leads to information gathering from friends, newspapers, television, and the internet. This study was not registered.
Strengths within the Community Perceived by Older Adults Living Alone in a Semi-Mountainous Rural Region: A Qualitative Study
Authors: Nakai Ai , Morioka Ikuharu
Published: April 2024

It is recommended that health promotion activities in the community focus on residents’ strengths. Hence, this study explored the community strengths perceived by older adults living alone in a semi-mountainous rural region of Japan. A qualitative, descriptive approach was used. Content analysis was performed using data obtained through face-to-face interviews. Interview data were coded; codes were classified based on similarity to create subcategories and categories. The strengths within the community, as perceived by older adults living alone in a semi-mountainous rural region, were revealed in four categories related to ten subcategories: “loose connections with others”, “active community participation”, “close relationships with community professionals”, and “familiarity with the living environment”. Strengths within the community perceived by older adults living alone in a semi-mountainous rural region were cultivated in an environment formed by their past lives. Utilizing these resources may help support community-based societies in semi-mountainous rural regions where depopulation and aging are expected to continue in the future. This study was not registered.
Supplementation of SkQ1 Increases Mouse In Vitro Oocyte Maturation and Subsequent Embryonic Development by Reducing Oxidative Stress
Authors: Li Zheng , Zhang Yiqian , Cao Jinping , Xing Xupeng , Liang Yalin , Zhang Yuxing , Tang Xiaopeng , Lin Shengyi , Wu Zhenfang , Li Zicong , Huang Sixiu
Published: April 2024

In vitro oocyte maturation (IVM) technology is important for assisted animal and human reproduction. However, the maturation rates and developmental potential of in vitro-matured oocytes are usually lower than those of in vivo-matured oocytes. Oxidative stress is a main factor that causes the lower maturation rates and quality of in vitro-matured oocytes. The purpose of this study was to investigate the effects of treatment with SkQ1, a mitochondria-targeted antioxidant, on mouse IVM and subsequent embryonic development. The results demonstrated that the supplementation of SkQ1 during IVM improves the maturation rates of mouse oocytes and the subsequent developmental competence of in vitro-fertilized embryos. The addition of SkQ1 to the IVM medium also decreased oxidative stress and apoptosis, and increased mitochondrial membrane potential in matured mouse oocytes. This study provides a new method through which to enhance the maturation rates and the quality of in vitro-matured mouse oocytes, thus promoting the application and development of assisted animal and human reproductive technology.
Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts
Authors: Yun Hyung-Mun , Kim Eonmi , Kwon Yoon-Ju , Park Kyung-Ran
Published: April 2024

Antioxidant vanillin (4-hydroxy-3-methoxybenzaldehyde) is used as a flavoring in foods, beverages, and pharmaceuticals. Vanillin possesses various biological effects, such as antioxidant, anti-inflammatory, antibacterial, and anticancer properties. This study aimed to investigate the biological activities of vanillin purified from Adenophora triphylla var. japonica Hara on bone-forming processes. Vanillin treatment induced mineralization as a marker for mature osteoblasts, after stimulating alkaline phosphatase (ALP) staining and activity. The bone-forming processes of vanillin are mainly mediated by the upregulation of the bone morphogenetic protein 2 (BMP2), phospho-Smad1/5/8, and runt-related transcription factor 2 (RUNX2) pathway during the differentiation of osteogenic cells. Moreover, vanillin promoted osteoblast-mediated bone-forming phenotypes by inducing migration and F-actin polymerization. Furthermore, we validated that vanillin-mediated bone-forming processes were attenuated by noggin and DKK1. Finally, we demonstrated that vanillin-mediated antioxidant effects prevent the death of osteoblasts during bone-forming processes. Overall, vanillin has bone-forming properties through the BMP2-mediated biological mechanism, indicating it as a bone-protective compound for bone health and bone diseases such as periodontitis and osteoporosis.
Proton-Pump Inhibitors in Eosinophilic Esophagitis: A Review Focused on the Role of Pharmacogenetics
Authors: Rodríguez-Alcolado Leticia , Navarro Pilar , Arias-González Laura , Grueso-Navarro Elena , Lucendo Alfredo J. , Laserna-Mendieta Emilio J.
Published: April 2024

Proton-pump inhibitors (PPIs) are the most administered first-line treatment for eosinophilic esophagitis (EoE). However, only around half of EoE patients respond histologically to a double dosage of PPI. In addition, 70% of responders maintain EoE in remission after tapering the PPI dose. In order to avoid endoscopy with biopsies—the only accurate method of assessing PPI response—efforts have been made to identify PPI responder patients. The clinical or endoscopic features and biomarkers evaluated so far, however, have not proven to be sufficient in predicting PPI response. Although new approaches based on omics technologies have uncovered promising biomarkers, the specialized and complex procedures required are difficult to implement in clinical settings. Alternatively, PPI pharmacogenetics based on identifying variations in CYP2C19 and STAT6 genes have shown promising results in EoE, and could easily be performed in most laboratories. Other genetic variations have also been associated with PPI response and may explain those cases not related to CYP2C19 or STAT6. Here, we provide an overview of PPI treatment in EoE and evidence of how genetic variations in CYP2C19 and other genes could affect PPI effectiveness, and also discuss studies evaluating the role of pharmacogenetics in predicting PPI response in patients with EoE.
Synergistic Effects of Chemotherapy and Phototherapy on Ovarian Cancer Using Follicle-Stimulating Hormone Receptor-Mediated Liposomes Co-Loaded with SN38 and IR820
Authors: Pian Lina , Zeng Bowen , Wang Nuoya , Wang Shuangqing , Wu Hao , Wan Hongshuang , Chen Liqing , Huang Wei , Gao Zhonggao , Jin Dan , Jin Mingji
Published: April 2024

We have developed an ovarian cancer-targeted drug delivery system based on a follicle-stimulating hormone receptor (FSHR) peptide. The lipophilic chemotherapeutic drug SN38 and the photosensitizer IR820 were loaded into the phospholipid bilayer of liposomes. The combination of chemotherapy and phototherapy has become a promising strategy to improve the therapeutic effect of chemotherapy drugs on solid tumors. IR820 can be used for photodynamic therapy (PDT), effectively converting near-infrared light (NIR) into heat and producing reactive oxygen species (ROS), causing damage to intracellular components and leading to cell death. In addition, PDT generates heat in near-infrared, thereby enhancing the sensitivity of tumors to chemotherapy drugs. FSH liposomes loaded with SN38 and IR820 (SN38/IR820-Lipo@FSH) were prepared using thin-film hydration-sonication. FSH peptide binding was analyzed using 1H NMR spectrum and Maldi-Tof. The average size and zeta potential of SN38/IR820-Lipo@FSH were 105.1 ± 1.15 nm (PDI: 0.204 ± 0.03) and −27.8 ± 0.42 mV, respectively. The encapsulation efficiency of SN38 and IR820 in SN38/IR820-Lipo@FSH liposomes were 90.2% and 91.5%, respectively, and their release was slow in vitro. FSH significantly increased the uptake of liposomes, inhibited cell proliferation, and induced apoptosis in A2780 cells. Moreover, SN38/IR820-Lipo@FSH exhibited better tumor-targeting ability and anti-ovarian cancer activity in vivo when compared with non-targeted SN38/IR820-Lipo. The combination of chemotherapy and photodynamic treatment based on an FSH peptide-targeted delivery system may be an effective approach to treating ovarian cancer.
Use of Plant Extracts in Polymeric Scaffolds in the Regeneration of Mandibular Injuries
Authors: de Oliveira Bruna Eduarda Gandra , Maia Fernanda Latorre Melgaço , Massimino Lívia Contini , Garcia Claudio Fernandes , Plepis Ana Maria de Guzzi , Martins Virgínia da Conceição Amaro , Reis Carlos Henrique Bertoni , Silva Vinícius Rodrigues , Bezerra Andre Alves , Pauris Carolina Chen , Buchaim Daniela Vieira , Silva Yggor Biloria e , Buchaim Rogerio Leone , da Cunha Marcelo Rodrigues
Published: April 2024

Severe loss of bone mass may require grafting, and, among the alternatives available, there are natural biomaterials that can act as scaffolds for the cell growth necessary for tissue regeneration. Collagen and elastin polymers are a good alternative due to their biomimetic properties of bone tissue, and their characteristics can be improved with the addition of polysaccharides such as chitosan and bioactive compounds such as jatoba resin and pomegranate extract due to their antigenic actions. The aim of this experimental protocol was to evaluate bone neoformation in experimentally made defects in the mandible of rats using polymeric scaffolds with plant extracts added. Thirty rats were divided into group 1, with a mandibular defect filled with a clot from the lesion and no graft implant (G1-C, n = 10); group 2, filled with collagen/chitosan/jatoba resin scaffolds (G2-CCJ, n = 10); and group 3, with collagen/nanohydroxyapatite/elastin/pomegranate extract scaffolds (G3-CHER, n = 10). Six weeks after surgery, the animals were euthanized and samples from the surgical areas were submitted to macroscopic, radiological, histological, and morphometric analysis of the mandibular lesion repair process. The results showed no inflammatory infiltrates in the surgical area, indicating good acceptance of the scaffolds in the microenvironment of the host area. In the control group (G1), there was a predominance of reactive connective tissue, while in the grafted groups (G2 and G3), there was bone formation from the margins of the lesion, but it was still insufficient for total bone repair of the defect within the experimental period standardized in this study. The histomorphometric analysis showed that the mean percentage of bone volume formed in the surgical area of groups G1, G2, and G3 was 17.17 ± 2.68, 27.45 ± 1.65, and 34.07 ± 0.64 (mean ± standard deviation), respectively. It can be concluded that these scaffolds with plant extracts added can be a viable alternative for bone repair, as they are easily manipulated, have a low production cost, and stimulate the formation of new bone by osteoconduction.
Topical Nanoemulsions as Delivery Systems for Green Extracts of Pterocaulon balansae Aiming at the Treatment of Sporotrichosis
Authors: Medeiros-Neves Bruna , Heidrich Daiane , Schuh Roselena Silvestri , Brazil Nathalya Tesch , Fachel Flávia Nathiely Silveira , Cassel Eduardo , Vargas Rubem Mário Figueiró , Scroferneker Maria Lúcia , von Poser Gilsane Lino , Koester Letícia Scherer , Teixeira Helder Ferreira
Published: April 2024

Coumarins are benzopyrones found in several plant genera, including Pterocaulon (Asteraceae). These compounds represent an important source of new treatments, especially as antimicrobial and antifungal agents. In this study, two coumarin-rich extracts from Pterocaulon balansae using green technologies were obtained through aqueous maceration (AE) and supercritical fluid extraction (SFE). Such extracts were incorporated into nanoemulsions (NAE and NSFE) composed of a medium-chain triglyceride oil core stabilized by phospholipids. The nanoemulsions exhibited droplet sizes between 127 and 162 nm, pH above 5.0, and viscosity of approximately 1.0 cP, properties compatible with the topical route. The coumarins permeation/retention from formulations through ear porcine skin using Franz-type diffusion cells were evaluated. Whatever the extract, coumarins were distributed in skin layers, especially in the dermis in both intact and impaired (tape stripping) skin. In addition, a significant increase in coumarins that reached up to the receptor fluid was observed for impaired skin, with increases of approximately threefold for NAE and fourfold for NSFE. Finally, antifungal activity of nanoemulsions was evaluated according to minimum inhibitory concentrations, and the values were 250 µg/mL for all strains tested. The overall results demonstrated the feasibility of incorporating P. balansae extracts into nanoemulsions and showed a potential alternative for the treatment of sporotrichosis.
Evolution of Telehealth—Its Impact on Palliative Care and Medication Management
Authors: Imam Syed N. , Braun Ursula K. , Garcia Mary A. , Jackson Leanne K.
Published: April 2024

Palliative care plays a crucial role in enhancing the quality of life for individuals facing serious illnesses, aiming to alleviate suffering and provide holistic support. With the advent of telehealth, there is a growing interest in leveraging technology to extend the reach and effectiveness of palliative care services. This article provides a comprehensive review of the evolution of telehealth, the current state of telemedicine in palliative care, and the role of telepharmacy and medication management. Herein we highlight the potential benefits, challenges, and future directions of palliative telemedicine. As the field continues to advance, the article proposes key considerations for future research, policy development, and clinical implementation, aiming to maximize the advantages of telehealth in assisting individuals and their families throughout the palliative care journey. The comprehensive analysis presented herein contributes to a deeper understanding of the role of telehealth in palliative care and serves as a guide for shaping its future trajectory.
Spin Angular Momentum at the Focus of a Superposition of an Optical Vortex and a Plane Wave with Linear Polarizations
Authors: Kotlyar Victor V. , Stafeev Sergey S. , Telegin Alexey M. , Kozlova Elena S.
Published: April 2024

In this paper, tight focusing of a superposition of a vortex laser beam with topological charge n with linear polarization and a plane wave with the same linear polarization directed along the horizontal axis is considered. Using the Richards–Wolf formalism, analytical expressions are obtained for the intensity distribution and longitudinal projection of the spin angular momentum in the focal plane. It is shown that for even and odd numbers n, the intensity and the spin angular momentum have different symmetries: for even n they are symmetric about both Cartesian axes, and for odd n they are symmetric only about the vertical axis. The intensity distribution has n local maxima at the focus, and it is nonzero on the optical axis for any n. The distribution of the longitudinal spin angular momentum (spin density) in the focal plane has (n + 2) subwavelength regions with a positive spin angular momentum and (n + 2) regions with a negative spin angular momentum, the centers of which alternately lie on a circle of a certain radius with a center on the optical axis. This spin distribution with different signs demonstrates the spin Hall effect at the focus. Negative and positive spins are mutually compensated, and the total spin is equal to zero at the focus. We have shown that by changing the topological charge of the optical vortex, it is possible to control the spin Hall effect at the focus, that is, to change the number of regions with spins of different signs.
Comparative Morpho-Physiological, Biochemical, and Gene Expressional Analyses Uncover Mechanisms of Waterlogging Tolerance in Two Soybean Introgression Lines
Authors: Sharmin Ripa Akter , Karikari Benjamin , Bhuiyan Mashiur Rahman , Kong Keke , Yu Zheping , Zhang Chunting , Zhao Tuanjie
Published: April 2024

Waterlogging is one of the key abiotic factors that severely impedes the growth and productivity of soybeans on a global scale. To develop soybean cultivars that are tolerant to waterlogging, it is a prerequisite to unravel the mechanisms governing soybean responses to waterlogging. Hence, we explored the morphological, physiological, biochemical, and transcriptional changes in two contrasting soybean introgression lines, A192 (waterlogging tolerant, WT) and A186 (waterlogging sensitive, WS), under waterlogging. In comparison to the WT line, waterlogging drastically decreased the root length (RL), shoot length (ShL), root fresh weight (RFW), shoot fresh weight (ShFW), root dry weight (RDW), and shoot dry weight (ShDW) of the WS line. Similarly, waterlogging inhibited soybean plant growth by suppressing the plant’s photosynthetic capacity, enhancing oxidative damage from reactive oxygen species, and decreasing the chlorophyll content in the WS line but not in the WT line. To counteract the oxidative damage and lipid peroxidation, the WT line exhibited increased activity of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), as well as higher levels of proline content than the WS line. In addition, the expression of antioxidant enzyme genes (POD1, POD2, FeSOD, Cu/ZnSOD, CAT1, and CAT2) and ethylene-related genes (such as ACO1, ACO2, ACS1, and ACS2) were found to be up-regulated in WT line under waterlogging stress conditions. In contrast, these genes showed a down-regulation in their expression levels in the stressed WS line. The integration of morpho-physiological, biochemical, and gene expression analyses provide a comprehensive understanding of the responses of WT and WS lines to waterlogging conditions. These findings would be beneficial for the future development of soybean cultivars that can withstand waterlogging.
MtTCP18 Regulates Plant Structure in Medicago truncatula
Authors: Su Xiaoyue , Zheng Junzan , Diao Xiaoxuan , Yang Zhongyi , Yu Deyue , Huang Fang
Published: April 2024

Plant structure has a large influence on crop yield formation, with branching and plant height being the important factors that make it up. We identified a gene, MtTCP18, encoding a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor highly conserved with Arabidopsis gene BRC1 (BRANCHED1) in Medicago truncatula. Sequence analysis revealed that MtTCP18 included a conserved basic helix–loop–helix (BHLH) motif and R domain. Expression analysis showed that MtTCP18 was expressed in all organs examined, with relatively higher expression in pods and axillary buds. Subcellular localization analysis showed that MtTCP18 was localized in the nucleus and exhibited transcriptional activation activity. These results supported its role as a transcription factor. Meanwhile, we identified a homozygous mutant line (NF14875) with a mutation caused by Tnt1 insertion into MtTCP18. Mutant analysis showed that the mutation of MtTCP18 altered plant structure, with increased plant height and branch number. Moreover, we found that the expression of auxin early response genes was modulated in the mutant. Therefore, MtTCP18 may be a promising candidate gene for breeders to optimize plant structure for crop improvement.
Introduction of Native Submerged Macrophytes to Restore Biodiversity in Streams
Authors: Van der Cruysse Lucas , De Cock Andrée , Lock Koen , Boets Pieter , Goethals Peter L. M.
Published: April 2024

Streams are biodiversity hotspots that provide numerous ecosystem services. Safeguarding this biodiversity is crucial to uphold sustainable ecosystem functioning and to ensure the continuation of these ecosystem services in the future. However, in recent decades, streams have witnessed a disproportionate decline in biodiversity compared to other ecosystems, and are currently considered among the most threatened ecosystems worldwide. This is the result of the combined effect of a multitude of stressors. For freshwater systems in general, these have been classified into five main pressures: water pollution, overexploitation, habitat degradation and destruction, alien invasive species, and hydromorphological pressures. On top of these direct stressors, the effects of global processes like environmental and climate change must be considered. The intricate and interconnected nature of various stressors affecting streams has made it challenging to formulate effective policies and management strategies. As a result, restoration efforts have not always been successful in creating a large-scale shift towards a better ecological status. In order to achieve an improved status in these systems, situation-specific management strategies tailored to specific stressor combinations may be needed. In this paper, we examine the potential of introducing native submerged macrophyte species to advance the restoration of stream ecosystems. Through successful introductions, we anticipate positive ecological outcomes, including enhanced water quality and increased biodiversity. This research is significant, as the potential success in restoring stream biodiversity not only represents progress in ecological understanding but also offers valuable insights for future restoration and management strategies for these vital ecosystems.
Microstructural Approach Application for Morphological Change Determinations of Grapes during Drying
Authors: Senadeera Wijitha , Banks Jasmine , Adiletta Giuseppina , Brewer Kate
Published: April 2024

Grape dehydration is practiced widely in the food industry with large yields of sultanas produced globally. This paper proposes an investigation into the microstructure changes of grapes as they are dried by imaging specimens at intervals during dehydration at two temperatures using scanning electron microscopy. Two main methods were developed to obtain the complex boundaries of cells present in grape tissue in over 36 SEM images. Segmentation of the binary image using an adapted watershed function obtained the most consistent and accurate morphological shape. This was compared to a secondary method which used Canny’s edge detection function, morphological closing and skeletonizing to outline the cellular microstructure. MATLAB was utilised to convert these boundaries into measurable areas so that quantitative data on average cell area, perimeter and cell axis lengths were acquired. It was found that over the drying time, the cell area and perimeter were reduced as expected. Some variability in the data was clear due to only single samples being analysed for each temperature and time combination. Trends in cell perimeter, diameter and shape will be used to demonstrate relationships between morphological structure, drying temperature, and duration. Detailed images of the microstructure were obtained, and a unique image processing algorithm was developed to quantitatively analyse the properties of this microstructure. The development of automatic image processing techniques and algorithms will enable quantitative data to be extracted from any image and extend to any plant/food material.
Integration of Urinary Peptidome and Fecal Microbiome to Explore Patient Clustering in Chronic Kidney Disease
Authors: Mavrogeorgis Emmanouil , Valkenburg Sophie , Siwy Justyna , Latosinska Agnieszka , Glorieux Griet , Mischak Harald , Jankowski Joachim
Published: April 2024

Millions of people worldwide currently suffer from chronic kidney disease (CKD), requiring kidney replacement therapy at the end stage. Endeavors to better understand CKD pathophysiology from an omics perspective have revealed major molecular players in several sample sources. Focusing on non-invasive sources, gut microbial communities appear to be disturbed in CKD, while numerous human urinary peptides are also dysregulated. Nevertheless, studies often focus on isolated omics techniques, thus potentially missing the complementary pathophysiological information that multidisciplinary approaches could provide. To this end, human urinary peptidome was analyzed and integrated with clinical and fecal microbiome (16S sequencing) data collected from 110 Non-CKD or CKD individuals (Early, Moderate, or Advanced CKD stage) that were not undergoing dialysis. Participants were visualized in a three-dimensional space using different combinations of clinical and molecular data. The most impactful clinical variables to discriminate patient groups in the reduced dataspace were, among others, serum urea, haemoglobin, total blood protein, urinary albumin, urinary erythrocytes, blood pressure, cholesterol measures, body mass index, Bristol stool score, and smoking; relevant variables were also microbial taxa, including Roseburia, Butyricicoccus, Flavonifractor, Burkholderiales, Holdemania, Synergistaceae, Enterorhabdus, and Senegalimassilia; urinary peptidome fragments were predominantly derived from proteins of collagen origin; among the non-collagen parental proteins were FXYD2, MGP, FGA, APOA1, and CD99. The urinary peptidome appeared to capture substantial variation in the CKD context. Integrating clinical and molecular data contributed to an improved cohort separation compared to clinical data alone, indicating, once again, the added value of this combined information in clinical practice.
The Circularity of Materials from the Perspective of a Product Life Cycle: A Life Cycle Assessment Case Study of Secondary Fence Boards—Part 1 (Baseline Scenario)
Authors: Kulczycka Joanna , Lewandowska Anna , Joachimiak-Lechman Katarzyna , Kurczewski Przemysław
Published: April 2024

In the era of the circular economy, solutions aimed at increasing the circularity of materials and products are highly welcome. Eco-design and waste management strategies are crucial for ensuring circularity and resource-saving. Strategies should be driven by assessing life cycle-based environmental performance. Tools to measure this performance should take into account two recycling-oriented parameters: recycled content and recycling rate. This paper presents the results of a life cycle assessment case study for a secondary fence board (baseline scenario). The circular footprint formula has been used to allocate burdens and credits between the supplier and the user of recycled materials. The potential environmental impact and the most significant issues have been calculated, identified, and presented. A general recommendation for further environmental development of the secondary fence board is to improve the production-related energy efficiency of recycling processes and increase the recycling rate of the board (to avoid landfilling).
Space Domain Awareness Observations Using the Buckland Park VHF Radar
Authors: Holdsworth David A. , Spargo Andrew J. , Reid Iain M. , Adami Christian L.
Published: April 2024

There is increasing interest in space domain awareness worldwide, motivating investigation of the use of non-traditional sensors for space surveillance. One such class of sensor is VHF wind profiling radars, which have a low cost relative to other radars typically applied to this task. These radars are ubiquitous throughout the world and may potentially offer complementary space surveillance capabilities to the Space Surveillance Network. This paper updates an initial investigation on the use of Buckland Park VHF wind profiling radars for observing resident space objects in low Earth orbit to further investigate the space surveillance capabilities of the sensor class. The radar was operated during the Australian Defence “SpaceFest” 2019 activity, incorporating new beam scheduling and signal processing functionality that extend upon the capabilities described in the initial investigation. The beam scheduling capability used two-line element propagations to determine the appropriate beam direction to use to observe transiting satellites. The signal processing capabilities used a technique based on the Keystone transform to correct for range migration, allowing the development of new signal processing modes that allow the coherent integration time to be increased to improve the SNR of the observed targets, thereby increasing the detection rate. The results reveal that 5874 objects were detected over 10 days, with 2202 unique objects detected, representing a three-fold increase in detection rate over previous single-beam direction observations. The maximum detection height was 2975.4 km, indicating a capability to detect objects in medium Earth orbit. A minimum detectable RCS at 1000 km of −10.97 dBm2 (0.09 m2) was observed. The effects of Faraday rotation resulting from the use of linearly polarised antennae are demonstrated. The radar’s utility for providing total electron content (TEC) measurements is investigated using a high-range resolution mode and high-precision ephemeris data. The short-term Fourier transform is applied to demonstrate the radar’s ability to investigate satellite rotation characteristics and monitor ionospheric plasma waves and instabilities.
Infrared Maritime Small-Target Detection Based on Fusion Gray Gradient Clutter Suppression
Authors: Wang Wei , Li Zhengzhou , Siddique Abubakar
Published: April 2024

The long-distance ship target turns into a small spot in an infrared image, which has the characteristics of small size, weak intensity, limited texture information, and is easily affected by noise. Moreover, the presence of heavy sea clutter, including sun glints that exhibit local contrast similar to small targets, negatively impacts the performance of small-target detection methods. To address these challenges, we propose an effective detection scheme called fusion gray gradient clutter suppression (FGGCS), which leverages the disparities in grayscale and gradient between the target and its surrounding background. Firstly, we designed a harmonic contrast map (HCM) by using the two-dimensional difference of Gaussian (2D-DoG) filter and eigenvalue harmonic mean of the structure tensor to highlight high-contrast regions of interest. Secondly, a local gradient difference measure (LGDM) is designed to distinguish isotropic small targets from background edges with local gradients in a specific direction. Subsequently, by integrating the HCM and LGDM, we designed a fusion gray gradient clutter suppression map (FGGCSM) to effectively enhance the target and suppress clutter from the sea background. Finally, an adaptive constant false alarm threshold is adopted to extract the targets. Extensive experiments on five real infrared maritime image sequences full of sea glints, including a small target and sea–sky background, show that FGGCS effectively increases the signal-to-clutter ratio gain (SCRG) and the background suppression factor (BSF) by more than 22% and 82%, respectively. Furthermore, its receiver operating characteristic (ROC) curve has an obviously more rapid convergence rate than those of other typical detection algorithms and improves the accuracy of small-target detection in complex maritime backgrounds.
Advanced Soil Organic Matter Prediction with a Regional Soil NIR Spectral Library Using Long Short-Term Memory–Convolutional Neural Networks: A Case Study
Authors: Miao Tianyu , Ji Wenjun , Li Baoguo , Zhu Xicun , Yin Jianxin , Yang Jiajie , Huang Yuanfang , Cao Yan , Yao Dongheng , Kong Xiangbin
Published: April 2024

Soil analysis using near-infrared spectroscopy has shown great potential to be an alternative to traditional laboratory analysis, and there is continuously increasing interest in building large-scale soil spectral libraries (SSLs). However, due to issues such as high non-linearity in soil spectral data and complexity in soil spatial variation, the establishment of robust prediction models for soil spectral libraries remains a challenge. This study aimed to investigate the performance of deep learning algorithms, including long short-term memory (LSTM) and LSTM–convolutional neural networks (LSTM–CNN) integrated models, to predict the soil organic matter (SOM) of a provincial-scale SSL, and compare it to the normally used local weighted regression (LWR) model. The Hebei soil spectral library (HSSL) contains 425 topsoil samples (0–20 cm), of which every 3 soil samples were collected from dry land, irrigated land, and paddy fields, respectively, in different counties of Hebei Province, China. The results show that the accuracy of the validation dataset rank as follows: LSTM–CNN (R2p = 0.96, RMSEp = 1.66 g/kg) > LSTM (R2p = 0.83, RMSEp = 3.42 g/kg) > LWR (R2p = 0.82, RMSEp = 3.79 g/kg). The LSTM–CNN model performed the best, mainly due to its comprehensive ability to effectively extract spatial and temporal features. Meanwhile, the LSTM model achieved higher accuracy than the LWR model, owing to its built-in memory unit and its advantage of faster feature band extraction. Thus, it was suggested to use deep learning algorithms for SOM predictions in SSLs. However, their performance on larger-scale SSLs such as continental/global SSLs still needs to be further investigated.
Injuries and Persistent Pain in Elite Adolescent Archery Athletes: A Cross-Sectional Epidemiological Study
Authors: Vasilis Nikolaos , Kyriakides Athanasios , Vasilopoulos George , Chatzitimotheou Maria , Gonidakis Grigorios , Kotsakis Athanasios , Paraskevopoulos Eleftherios , Kapreli Eleni
Published: April 2024

This cross-sectional epidemiological study aimed to evaluate the prevalence of injuries among young archers engaged in high-intensity training during the European Youth Championship. A total of 200 participants (104 males/96 females) from 34 countries were included, with a mean age of 16.9 years and average competitive experience of 6.5 years. Structured questionnaires, administered by four physiotherapist interviewers, gathered comprehensive data. Results revealed that 43.5% of participants experienced shoulder pain during training, highlighting the vulnerability of upper limbs in archers. Additionally, 30% required medications to facilitate training, underscoring the impact of injuries on continued participation. Physiotherapy was utilized by 52.3% of participants, emphasizing the need for therapeutic intervention. Furthermore, 31.8% had to cease training due to injuries, indicating a substantial hindrance to athletic progression. The mean pain duration was 3.9 months, with an average intensity of 5.94, and 8% exhibited symptoms of central sensitization. In conclusion, this study demonstrates a noteworthy prevalence of injuries, particularly in the upper limbs, among young archers undergoing intensive training. The findings underscore the importance of targeted injury prevention strategies and comprehensive rehabilitation approaches to ensure the well-being and sustained participation of young athletes in competitive archery.