bluuebunny/crossref_metadata_2025 huggingface dataset:
Scalable Precursor-Assisted Synthesis of a High Voltage LiNiyCo1−yPO4 Cathode for Li-Ion Batteries
Authors: Islam Mobinul , Ali Ghulam , Faizan Muhammad , Han Daseul , Ali Basit , Yun Sua , Ahmad Haseeb , Nam Kyung-Wan
Published: December 2023

A solid-solution cathode of LiCoPO4-LiNiPO4 was investigated as a potential candidate for use with the Li4Ti5O12 (LTO) anode in Li-ion batteries. A pre-synthesized nickel–cobalt hydroxide precursor is mixed with lithium and phosphate sources by wet ball milling, which results in the final product, LiNiyCo1−yPO4 (LNCP) by subsequent heat treatment. Crystal structure and morphology of the product were analyzed by X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM). Its XRD patterns show that LNCP is primarily a single-phase compound and has olivine-type XRD patterns similar to its parent compounds, LiCoPO4 and LiNiPO4. Synchrotron X-ray absorption spectroscopy (XAS) analysis, however, indicates that Ni doping in LiCoPO4 is unfavorable because Ni2+ is not actively involved in the electrochemical reaction. Consequently, it reduces the charge storage capability of the LNCP cathode. Additionally, ex situ XRD analysis of cycled electrodes confirms the formation of the electrochemically inactive rock salt-type NiO phase. The discharge capacity of the LNCP cathode is entirely associated with the Co3+/Co2+ redox couple. The electrochemical evaluation demonstrated that the LNCP cathode paired with the LTO anode produced a 3.12 V battery with an energy density of 184 Wh kg−1 based on the cathode mass.
A Comprehensive Ecotoxicity Study of Molybdenum Disulfide Nanosheets versus Bulk form in Soil Organisms
Authors: Santos Joana , Barreto Angela , Fernandes Cristiana , Silva Ana Rita R. , Cardoso Diogo N. , Pinto Edgar , Daniel-da-Silva Ana L. , Maria Vera L.
Published: December 2023

The increasing use of molybdenum disulfide (MoS2) nanoparticles (NPs) raises concerns regarding their accumulation in soil ecosystems, with limited studies on their impact on soil organisms. Study aim: To unravel the effects of MoS2 nanosheets (two-dimensional (2D) MoS2 NPs) and bulk MoS2 (156, 313, 625, 1250, 2500 mg/kg) on Enchytraeus crypticus and Folsomia candida. The organisms’ survival and avoidance behavior remained unaffected by both forms, while reproduction and DNA integrity were impacted. For E. crypticus, the individual endpoint reproduction was more sensitive, increasing at lower concentrations of bulk MoS2 and decreasing at higher ones and at 625 mg/kg of 2D MoS2 NPs. For F. candida, the molecular endpoint DNA integrity was more impacted: 2500 mg/kg of bulk MoS2 induced DNA damage after 2 days, with all concentrations inducing damage by day 7. 2D MoS2 NPs induced DNA damage at 156 and 2500 mg/kg after 2 days, and at 1250 and 2500 mg/kg after 7 days. Despite affecting the same endpoints, bulk MoS2 induced more effects than 2D MoS2 NPs. Indeed, 2D MoS2 NPs only inhibited E. crypticus reproduction at 625 mg/kg and induced fewer (F. candida) or no effects (E. crypticus) on DNA integrity. This study highlights the different responses of terrestrial organisms to 2D MoS2 NPs versus bulk MoS2, reinforcing the importance of risk assessment when considering both forms.
Advanced Nanoscale Materials for Thermoelectric Applications
Authors: Zhang Ting
Published: December 2023

Recently, there has been growing academic interest in researching thermoelectric materials that exhibit energy conversion capability between thermal energy and electricity, providing solutions to energy crises and environmental pollution [...]
Coating of Filter Materials with CeO2 Nanoparticles Using a Combination of Aerodynamic Spraying and Suction
Authors: Abramova Anna V. , Kozlov Daniil A. , Veselova Varvara O. , Kozlova Taisiya O. , Ivanova Olga S. , Mikhalev Egor S. , Voytov Yuri I. , Baranchikov Alexandr E. , Ivanov Vladimir K. , Cravotto Giancarlo
Published: December 2023

Textiles and nonwovens (including those used in ventilation systems as filters) are currently one of the main sources of patient cross-infection. Healthcare-associated infections (HAIs) affect 5–10% of patients and stand as the tenth leading cause of death. Therefore, the development of new methods for creating functional nanostructured coatings with antibacterial and antiviral properties on the surfaces of textiles and nonwoven materials is crucial for modern medicine. Antimicrobial filter technology must be high-speed, low-energy and safe if its commercialization and mass adoption are to be successful. Cerium oxide nanoparticles can act as active components in these coatings due to their high antibacterial activity and low toxicity. This paper focuses on the elaboration of a high-throughput and resource-saving method for the deposition of cerium oxide nanoparticles onto nonwoven fibrous material for use in air-conditioning filters. The proposed spraying technique is based on the use of an aerodynamic emitter and simultaneous suction. Cerium oxide nanoparticles have successfully been deposited onto the filter materials used in air conditioning systems; the antibacterial activity of the ceria-modified filters exceeded 4.0.
The Impact of Nutrient Supply on Prostate Cancer Risk Worldwide
Authors: Jiang Jinjiang , Yang Jie , Chen Bo , Li Jinze , Zhang Ting , Tan Daqing , Tang Bo , Wei Qiang
Published: December 2023

We aim to explore the association between nutrient supply and the incidence of prostate cancer globally. We utilized national nutrient supply data from the Food and Agriculture Organization of the United Nations for 150 countries, including the average supply of total protein (APS), animal protein (AAPS), fat (AFS), animal protein/total protein ratio (ATR), and share of dietary energy supply derived from cereals, roots, and tubers (CR). Prostate cancer incidence data were sourced from the Global Burden Disease 2019 (GBD2019). Correlation, regression analyses, and subgroup analysis were conducted. Our findings imply that incidence of prostate cancer is significantly correlated to APS (ρ = 0.394, p < 0.01), AAPS (ρ = 0.560, p < 0.01), AFS (ρ = 0.522, p < 0.01), ATR (ρ = 0.592, p < 0.01), and CR (ρ = −0.667, p < 0.01). After adjusting for confounders, regression analysis showed linear relationships between the AAPS (β = 0.605, p = 0.006), ATR (β = 70.76, p = 0.005), CR (β = −1.4451, p < 0.01), and age-standardized incidence rates (ASIRs) of prostate cancer, while no association was observed with APS (β = 0.030, p = 0.483) or AFS (β = 0.237, p = 0.405). Subgroup analysis suggested that dietary supply indicators were associated with ASIR in middle, middle-high, and high SDI, but not in countries with low and middle-low SDI. In summary, prostate cancer rates globally correlate significantly with AAPS, ATR, and CR, but not with APS and AFS. When considering the SDI of countries, the relationship is generally more pronounced in economically advanced nations, but not evident in low and middle-low SDI countries.
Dietary Strategies to Reduce Triglycerides in Women of Reproductive Age: A Simulation Modelling Study
Authors: Habibi Nahal , Leemaqz Shalem , Louie Jimmy Chun Yu , Wycherley Thomas P. , Grieger Jessica A.
Published: December 2023

Many women of reproductive age have poor diet quality and are at higher risk of chronic diseases such as diabetes. Triglycerides are a critical risk factor for chronic diseases, and although they can be influenced by diet, there are minimal dietary intervention studies identifying key foods/food groups that reduce triglycerides. We performed data simulation modelling to estimate the potential reductions in fasting triglycerides that could be achieved by different dietary strategies in reproductive age women. The model was created using data from the 2011–2013 Australian Health Survey and incorporated various factors such as demographics, nutrient intake, and plasma biomarkers. Multiple linear regression analysis was conducted to estimate triglyceride levels, considering nutrient intake and pre-determined covariates. Dietary scenarios were developed, reducing the consumption of processed/ultra-processed foods, while increasing the intake of minimally processed foods like fruits, vegetables, fish, and nuts. A total of 606 women were included. Reducing processed foods by 50% plus increasing intakes of fruits (75–225 g/day), vegetables (75–225 g/day), or nuts (10–40 g/day) decreased triglycerides by up to 4.3%. Additionally, incorporating 80 g/day of omega 3 fish (>800 mg long-chain omega 3/100 g) decreased triglycerides by 8.2%. The clinical relevance of lowering triglycerides for cardiometabolic disease management should be tested in dietary intervention studies in women.
Microencapsulated Limosilactobacillus reuteri Encoding Lactoferricin-Lactoferrampin Targeted Intestine against Salmonella typhimurium Infection
Authors: Wang Xueying , Xie Weichun , Cai Limeng , Han Chuang , Kuang Hongdi , Shao Yilan , Zhang Senhao , Zhang Qi , Li Jiaxuan , Cui Wen , Jiang Yanping , Tang Lijie
Published: December 2023

Salmonella enterica serovar Typhimurium (S. typhimurium) is an important foodborne pathogen that infects both humans and animals and develops acute gastroenteritis. As porcine intestines are relatively similar to the human ones due to their relatively similar sizes and structural similarity, S. typhimurium causes analogous symptoms in both. Novel strategies for controlling S. typhimurium infection are also desired, such as mucosal-targeted delivery of probiotics and antimicrobial peptides. The bovine lactoferricin-lactoferrampin-encoding Limosilactobacillus reuteri (LR-LFCA) strain improves intestinal barrier function by strengthening the intestinal barrier. Weaned piglets were selected for oral administration of microencapsulated LR-LFCA (microcapsules entrap LR-LFCA into gastro-resistant polymers) and then infected with S. typhimurium for 3 days. We found that orally administering microencapsulated LR-LFCA to weaned piglets attenuated S. typhimurium-induced production of inflammatory factors in the intestinal mucosa by inhibiting the nuclear factor-kappa B (NF-κB) and P38 mitogen-activated protein kinases (MAPK) signaling pathway. Moreover, microencapsulated LR-LFCA administration significantly suppressed the oxidative stress that may correlate with gut microbiota (reduced Salmonella population and increased α-diversity and Lactobacillus abundance) and intestinal function (membrane transport and metabolism). Our work demonstrated that microencapsulated LR-LFCA effectively targeted intestine delivery of Lactobacillus and antimicrobial peptides and modulated gut microbiota and mucosal immunity. This study reveals a novel targeting mucosal strategy against S. typhimurium infection.
Assessment of Differential Perceptions of Core Nursing Competencies between Nurse Managers and Nursing Graduates: A Cross-Sectional Study
Authors: Tarabeih Mahdi , Na’amnih Wasef
Published: December 2023

Background: The literature review on perceptions of nursing competencies shows a critical shortage of studies addressing this topic. Aim: To examine and compare perceptions of important nursing competencies of nursing graduates, and nurse managers. Methods: A cross-sectional study was conducted among 148 students, who were recent graduates of the nursing school with RN degrees and had completed advanced training lasting 3 months at the Sheba Academic School of Nursing and the Academic School of Tel Aviv-Yafo, and 183 nurse managers with at least one year of seniority in the position in hospitals and community clinics in Israel. The recruitment and data collection of the nurse managers lasted 12 months, from July 2021 to July 2022, and for nursing graduates, two months, from June 2022 to July 2022. An online questionnaire was constructed and validated by five professional and experienced individuals in the research domains for adequate content validity. The questionnaire consisted of 47 items in total. Domains included: (1) professionalism in research; (2) skills for direct treatment; (3) support and communication; (4) professional knowledge; (5) personal abilities; and (6) critical thinking and innovation. Results: Overall, 331 valid questionnaires were collected (85% response rate). The difference in the nurse’s research professionalism index was found to be statistically significant with a higher rating given by nurse managers, p = 0.003. However, the difference in the direct treatment index of nurses was found to be statistically not significant, p = 0.610, between newly graduated nurses and nurse managers. The independent sample t-test indicated a significant difference with a higher rating among nurse managers in the nurse’s support and communication index, p = 0.020; professional knowledge index, p < 0.001; personal abilities index, p < 0.001; and critical thinking index, p = 0.006, between newly graduated nurses and nurse managers. Conclusion: Nursing education programs aimed at training future nurses with core competences should include a curriculum designed to promote the acquisition of these six core competences leading to a congruence between the role expectations of nurse managers and those of newly graduated nurses.
Pediatric Ocular Health and Obstructive Sleep Apnea Syndrome: A Review
Authors: Zaffanello Marco , Bonacci Erika , Piacentini Giorgio , Nosetti Luana , Pedrotti Emilio
Published: December 2023

Obstructive sleep apnea (OSA) affects neurobehavioral, cognitive, and cardiovascular aspects, particularly in children, by obstructing the upper airways during sleep. While its impact in adult ocular health is recognized, there is ongoing debate about OSA’s relevance in pediatrics. This review explores the relationship between OSA and ocular health in children, focusing on the effects and potential improvements through treatment. A systematic search found 287 articles through PubMeD/MEDLINE, Scopus, Web of Science, and ScienceDirect; 94.4% were excluded. After careful selection, six English articles were included, addressing the effects of OSA on children’s eyes. Three studies examined choroidal alterations, three explored retinal and optic nerve changes, and two analyzed ocular changes following otorhinolaryngological intervention. The immediate correlation in children is inconclusive, but age may be a contributing factor. Pediatric OSA patients exhibit corneal anomalies and increased optic nerve thickness, possibly due to intermittent hypoxia. OSA influences retinal vascular density in children, with increased density after treatment and reduced choroidal thickness in cases of adenotonsillar hypertrophy. This review emphasized OSA’s significant impact on children’s ocular health, revealing alterations in the optic nerve, choroid, retina, and cornea. While the direct correlation with the optic nerve is not always evident, OSA raises intraocular pressure and induces structural changes. Treatment holds promise, highlighting the need for regular monitoring to promptly address childhood OSA.
In Silico, In Vitro, and In Vivo Evaluation of Caffeine-Coated Nanoparticles as a Promising Therapeutic Avenue for AML through NF-Kappa B and TRAIL Pathways Modulation
Authors: Siddique Muhammad Hamid , Bukhari Sidra , Khan Inam Ullah , Essa Asiya , Ali Zain , Sabir Usama , Ayoub Omiya , Saadia Haleema , Yaseen Muhammad , Sultan Aneesa , Murtaza Iram , Kerr Philip G. , Bhat Mashooq Ahmad , Anees Mariam
Published: December 2023

Background: Advancements in nanoscience have led to a profound paradigm shift in the therapeutic applications of medicinally important natural drugs. The goal of this research is to develop a nano-natural product for efficient cancer treatment. Methods and Results: For this purpose, mesoporous silica nanoparticles (MSNPs) were formulated, characterized, and loaded with caffeine to develop a targeted drug delivery system, i.e., caffeine-coated nanoparticles (CcNPs). In silico docking studies were conducted to examine the binding efficiency of the CcNPs with different apoptotic targets followed by in vitro and in vivo bioassays in respective animal models. Caffeine, administered both as a free drug and in nanomedicine form, along with doxorubicin, was delivered intravenously to a benzene-induced AML model. The anti-leukemic potential was assessed through hematological profiling, enzymatic biomarker analysis, and RT-PCR examination of genetic alterations in leukemia markers. Docking studies show strong inter-molecular interactions between CcNPs and apoptotic markers. In vitro analysis exhibits statistically significant antioxidant activity, whereas in vivo analysis exhibits normalization of the genetic expression of leukemia biomarkers STMN1 and S1009A, accompanied by the restoration of the hematological and morphological traits of leukemic blood cells in nanomedicine-treated rats. Likewise, a substantial improvement in hepatic and renal biomarkers is also observed. In addition to these findings, the nanomedicine successfully normalizes the elevated expression of GAPDH and mTOR induced by exposure to benzene. Further, the nanomedicine downregulates pro-survival components of the NF-kappa B pathway and upregulated P53 expression. Additionally, in the TRAIL pathway, it enhances the expression of pro-apoptotic players TRAIL and DR5 and downregulates the anti-apoptotic protein cFLIP. Conclusions: Our data suggest that MSNPs loaded with caffeine, i.e., CcNP/nanomedicine, can potentially inhibit transformed cell proliferation and induce pro-apoptotic TRAIL machinery to counter benzene-induced leukemia. These results render our nanomedicine as a potentially excellent therapeutic agent against AML.
Uncommon Septic Arthritis of the Hip Joint in an Immunocompetent Adult Patient Due to Bacillus pumilus and Paenibacillus barengoltzii Managed with Long-Term Treatment with Linezolid: A Case Report and Short Literature Review
Authors: Cismasiu Razvan Silviu , Birlutiu Rares-Mircea , Preoțescu Liliana Lucia
Published: December 2023

The Bacillus and Paenibacillus genera are diverse soil-related bacterial pathogens. In this case report, we describe, to our knowledge, the first report of septic arthritis in a native hip joint in an immunocompetent adult patient caused by Bacillus pumilus and Paenibacillus barengoltzii. We describe the case of a 39-year-old Caucasian male patient who sought medical advice for chronic pain on the mobilization of the right hip, decreased range of motion, and physical asthenia. The patient underwent a surgical intervention (core decompression) for a right osteonecrosis of the femoral head, with a slightly favorable postoperative evolution after surgery for one month. Surgical treatment was planned on the basis of clinical and paraclinical investigations and the joint damage. The hip was explored using an anterior approach under spinal anesthesia and standard antibiotic prophylaxis. After resection of the femoral head, meticulous debridement of all inflammatory tissues was performed, and a preformed temporary spacer was inserted into the femoral canal. Bacteriological laboratory studies identified Bacillus pumilus and Paenibacillus barengoltzii via matrix-assisted laser desorption–ionization time-of-flight mass spectrometry analysis. The patient initially received nine days of empirical therapy with intravenous antibiotics (linezolid and meropenem). After the bacterial strains were identified, the patient received organism-specific antibiotic therapy with the same antibiotics and dose for eight days until discharge. After discharge, the patient was referred to another hospital, where he continued treatment with linezolid for seven weeks and, after that, four weeks of oral therapy with cotrimoxazole and rifampicin. During this period, no severe or potentially life-threatening adverse events were recorded during long-term treatment with linezolid or with the two oral antibiotics. In conclusion, our findings suggest that long-term treatment with linezolid may be a viable option for the management of bone and joint infections caused by Bacillus pumilus and Paenibacillus barengoltzii.
Helpful, Unnecessary, or Harmful: A Systematic Review of the Effects of Prescription Drug Monitoring Program Use on Opioid Prescriptions
Authors: Smith Nina Z. Y. , Thornton J. Douglas , Fenton Susan H. , Simmons Debora , Champagne-Langabeer Tiffany
Published: December 2023

Prescription drug misuse is a global problem, especially in the United States (US). Clinician involvement is necessary in this crisis, and prescription drug monitoring programs (PDMPs) are a recommended tool for the prevention, recognition, and management of prescription opioid misuse. However, because of the plethora of differences between different PDMPs, research on their effects is mixed. Yet, despite varied evidence, policy on PDMP use is trending stricter and more comprehensive. We aimed to identify patterns in the research to inform clinicians and policy. Through a systematic review of four literature databases (CINAHL, Cochrane Database, Embase, and Medline/OVID), we found 56 experimental and quasi-experimental studies published between 2016 and 2023 evaluating PDMP effects on clinician behavior. To address study heterogeneity, we categorized studies by type of intervention and study outcome. The review suggests that more comprehensive PDMP legislation is associated with decreases in the number of opioid prescriptions overall and the number of risky prescriptions prescribed or dispensed. However, this review shows that much is still unknown, encourages improvements to PDMPs and policies, and suggests further research.
An In Vitro Assessment Method for Chemotherapy-Induced Peripheral Neurotoxicity Caused by Anti-Cancer Drugs Based on Electrical Measurement of Impedance Value and Spontaneous Activity
Authors: Han Xiaobo , Matsuda Naoki , Ishibashi Yuto , Shibata Mikako , Suzuki Ikuro
Published: December 2023

Chemotherapy-induced peripheral neurotoxicity (CIPN) is a major adverse event of anti-cancer drugs, which still lack standardized measurement and treatment methods. In the present study, we attempted to evaluate neuronal dysfunctions in cultured rodent primary peripheral neurons using a microelectrode array system. After exposure to typical anti-cancer drugs (i.e., paclitaxel, vincristine, oxaliplatin, and bortezomib), we successfully detected neurotoxicity in dorsal root ganglia neurons by measuring electrical activities, including impedance value and spontaneous activity. The impedance value decreased significantly for all compounds, even at low concentrations, which indicated cell loss and/or neurite degeneration. The spontaneous activity was also suppressed after exposure, which suggested neurotoxicity again. However, an acute response was observed for paclitaxel and bortezomib before toxicity, which showed different mechanisms based on compounds. Therefore, MEA measurement of impedance value could provide a simple assessment method for CIPN, combined with neuronal morphological changes.
Fluorinated Cell-Penetrating Peptide for Co-Delivering siHIF-1α and Sorafenib to Enhance In Vitro Anti-Tumor Efficacy
Authors: Wan Yu , Yang Yuhan , Lai Qiuyue , Wang Wangxia , Wu Mingyu , Feng Shun
Published: December 2023

Antiangiogenic therapy with sorafenib (SF) alone is ineffective in eradicating tumors, and its long-term application can exacerbate tumor hypoxia, which in turn restricts SF’s therapeutic efficacy. Here, a redox-responsive fluorinated peptide (DEN-TAT-PFC) consisting of dendritic poly-lysine, cell-penetrating peptide TAT, and perfluorocarbon was designed and synthesized to co-load siRNA-targeting hypoxia-inducible factors (siHIF-1α) and SF. The unique architecture of the peptide and fluorinated modifications enhanced the siRNA delivery efficiency, including increased siRNA binding, GSH-responsive release, cellular uptake, endosomal escape, and serum resistance. Simultaneously, the DEN-TAT-PFC/SF/siHIF-1α co-delivery system achieved efficient knockdown of HIF-1α at mRNA and protein levels, thus alleviating hypoxia and further substantially reducing VEGF expression. Additionally, the excellent oxygen-carrying ability of DEN-TAT-PFC may facilitate relief of the hypoxic microenvironment. As a result of these synergistic effects, DEN-TAT-PFC/SF/siHIF-1α exhibited considerable anti-tumor cell proliferation and anti-angiogenesis effects. Therefore, DEN-TAT-PFC can be a versatile platform for fabricating fluorine-containing drugs/siRNA complex nano-systems.
Preparation of trans-Crocetin with High Solubility, Stability, and Oral Bioavailability by Incorporation into Three Types of Cyclodextrins
Authors: Liu Nan , Xiao Jie , Zang Ling-He , Quan Peng , Liu Dong-Chun
Published: December 2023

Crocetin (CRT), an active compound isolated from saffron, exhibits several pharmacological activities, including anti-tumor and immune-regulatory activities, and is effective against myocardial ischemia and coronary heart disease; however, its low stability and solubility limit its clinical application. Therefore, we investigated CRT inclusion complexes (ICs) with three cyclodextrins—α-CD, HP-β-CD, and γ-CD—suitable for oral administration prepared using an ultrasonic method. Fourier transform infrared spectroscopy and powder X-ray diffraction indicated that the crystalline state of CRT in ICs disappeared, and intermolecular interactions were observed between CRT and CDs. 1H nuclear magnetic resonance and phase solubility studies confirmed CRT encapsulation in the CD cavity and the formation of ICs. In addition, we observed the morphology of ICs using scanning electron microscopy. All ICs showed a high drug encapsulation efficiency (approximately 90%) with 6500–10,000 times better solubilities than those of the pure drug. CRT showed rapid dissolution, whereas pure CRT was water-insoluble. The formation of ICs significantly improved the storage stability of CRT under heat, light, and moisture conditions. Further, the peak time of CRT in rats significantly decreased, and the relative bioavailability increased by approximately 3–4 times. In addition, the oral bioavailability of CRT IC was evaluated. Notably, the absorption rate and degree of the drug in rats were improved. This study illustrated the potential applications of CRT/CD ICs in the food, healthcare, and pharmaceutical industries, owing to their favorable dissolution, solubility, stability, and oral bioavailability.
Rapid Study on Mefloquine Hydrochloride Complexation with Hydroxypropyl-β-Cyclodextrin and Randomly Methylated β-Cyclodextrin: Phase Diagrams, Nuclear Magnetic Resonance Analysis, and Stability Assessment
Authors: Durand Amaury , Mathiron David , Rigaud Sébastien , Djedaini-Pilard Florence , Marçon Frédéric
Published: December 2023

This study investigates the complexation of mefloquine hydrochloride by cyclodextrins to improve its solubility in order to design an oral solution. This approach may enhance the effectiveness of mefloquine, a drug which can be used for malaria prophylaxis and treatment in children. Mefloquine hydrochloride’s solubility was assessed in different buffer solutions, and its quantification was achieved through high-performance liquid chromatography. The complexation efficiency with cyclodextrins was evaluated, and nuclear magnetic resonance (NMR) methods were employed to determine the interactions between mefloquine and cyclodextrins. Mefloquine’s solubility increased when combined with hydroxypropyl-β-cyclodextrin (HP-β-CD) and randomly methylated β-cyclodextrin (RAMEB), with RAMEB being more effective. The drug’s solubility varied across different pH buffers, being higher in acidic buffers. Interestingly, mefloquine’s solubility decreased with a citrate buffer, possibly due to precipitation. The NMR studies highlighted non-covalent interactions between RAMEB, HP-β-CD, and mefloquine, explaining the solubilizing effect via complexation phenomena. Furthermore, the NMR experiments indicated the complexation of mefloquine by all the studied cyclodextrins, forming diastereoisomeric complexes. Cyclodextrin complexation improved mefloquine’s solubility, potentially impacting its bioavailability.
Transcriptome-Based Identification of the Optimal Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Analyses of Lingonberry Fruits throughout the Growth Cycle
Authors: Zhang Wanchen , Xu Jian , Wang Qiang , Li Jing , Li Yadong , Dong Mei , Sun Haiyue
Published: December 2023

(1) Background: Vaccinium vitis-idaea is a nutritionally and economically valuable natural wild plant species that produces berries useful for treating various diseases. There is growing interest in lingonberry, but there is limited information regarding lingonberry reference genes suitable for gene expression analyses of different tissues under various abiotic stress conditions. The objective of this study was to identify stable reference genes suitable for different lingonberry tissues in response to abiotic stress. (2) Methods: The delta Ct method and the GeNorm v3.5 and NormFinder v20 programs were used to comprehensively analyze gene expression stability. (3) Results: Actin Unigene23839 was the best reference gene for analyzing different cultivars, whereas Actin CL5740.Contig2 was the most suitable reference gene for analyzing different tissues and alkali stress. In contrast, 18S rRNA CL5051.Contig1 was the most stable reference gene under drought conditions. (4) Conclusions: These suitable reference genes may be used in future qRT-PCR analyses of different lingonberry tissues and the effects of abiotic stresses. Furthermore, the study data may be useful for functional genomics studies and the molecular breeding of lingonberry. In summary, internal reference genes or internal reference gene combinations should be carefully selected according to the experimental conditions to ensure that the generated gene expression data are accurate.
Genomic and Cytogenetic Analysis of Synthetic Polyploids between Diploid and Tetraploid Cotton (Gossypium) Species
Authors: Khidirov Mukhammad T. , Ernazarova Dilrabo K. , Rafieva Feruza U. , Ernazarova Ziraatkhan A. , Toshpulatov Abdulqahhor Kh. , Umarov Ramziddin F. , Kholova Madina D. , Oripova Barno B. , Kudratova Mukhlisa K. , Gapparov Bunyod M. , Khidirova Maftunakhan M. , Komilov Doniyor J. , Turaev Ozod S. , Udall Joshua A. , Yu John Z. , Kushanov Fakhriddin N.
Published: December 2023

Cotton (Gossypium spp.) is the most important natural fiber source in the world. The genetic potential of cotton can be successfully and efficiently exploited by identifying and solving the complex fundamental problems of systematics, evolution, and phylogeny, based on interspecific hybridization of cotton. This study describes the results of interspecific hybridization of G. herbaceum L. (A1-genome) and G. mustelinum Miers ex Watt (AD4-genome) species, obtaining fertile hybrids through synthetic polyploidization of otherwise sterile triploid forms with colchicine (C22H25NO6) treatment. The fertile F1C hybrids were produced from five different cross combinations: (1) G. herbaceum subsp. frutescens × G. mustelinum; (2) G. herbaceum subsp. pseudoarboreum × G. mustelinum; (3) G. herbaceum subsp. pseudoarboreum f. harga × G. mustelinum; (4) G. herbaceum subsp. africanum × G. mustelinum; (5) G. herbaceum subsp. euherbaceum (variety A-833) × G. mustelinum. Cytogenetic analysis discovered normal conjugation of bivalent chromosomes in addition to univalent, open, and closed ring-shaped quadrivalent chromosomes at the stage of metaphase I in the F1C and F2C hybrids. The setting of hybrid bolls obtained as a result of these crosses ranged from 13.8–92.2%, the fertility of seeds in hybrid bolls from 9.7–16.3%, and the pollen viability rates from 36.6–63.8%. Two transgressive plants with long fiber of 35.1–37.0 mm and one plant with extra-long fiber of 39.1–41.0 mm were identified in the F2C progeny of G. herbaceum subsp. frutescens × G. mustelinum cross. Phylogenetic analysis with 72 SSR markers that detect genomic changes showed that tetraploid hybrids derived from the G. herbaceum × G. mustelinum were closer to the species G. mustelinum. The G. herbaceum subsp. frutescens was closer to the cultivated form, and its subsp. africanum was closer to the wild form. New knowledge of the interspecific hybridization and synthetic polyploidization was developed for understanding the genetic mechanisms of the evolution of tetraploid cotton during speciation. The synthetic polyploids of cotton obtained in this study would provide beneficial genes for developing new cotton varieties of the G. hirsutum species, with high-quality cotton fiber and strong tolerance to biotic or abiotic stress. In particular, the introduction of these polyploids to conventional and molecular breeding can serve as a bridge of transferring valuable genes related to high-quality fiber and stress tolerance from different cotton species to the new cultivars.
Prediction of Aging Performance of Particle-Filled Polymer Composites Based on RVE Model
Authors: Wang Huizhen , Fang Congli , Zhang Yujiao , Zhang Minghua , Shen Tao , Du Jianke
Published: December 2023

In this study, the aging performance of particle-filled polymer composites (PFPCs) under thermo-oxidative conditions was investigated on multiple scales. High-temperature-accelerated tests were conducted to analyze the effects of aging time and temperature. A representative volume element (RVE) model was established for the PFPCs using a random particle-filling algorithm. A predictive model for the crosslink density was conducted based on the closed-loop chain reaction of polymer oxidation. According to the theory of polymer physics, the relation between the crosslink density and matrix modulus was determined. The particle/matrix interface in the RVE model was represented by the cohesive zone model (CZM). The parameters of the CZM were determined by the inversion techniques. Then, a comprehensive multiscale RVE model was constructed, which was applied to predict the modulus and dewetting strain of the aged PFPCs. The predicted results show good agreement with the test results, which verifies the reliability of our model.
Evaluation of the Mechanical and Tribological Behavior of Polyether Ether Ketone Fiber-Reinforced Resin-Based Friction Materials Fabricated by Wet Granulation
Authors: Li Lekai , Ma Zichao , Liu Guoqin , Song Wei , Ren Lili , Yuan Shengwang , Yang Xiao , Zhang Qifeng , Ma Yunhai
Published: December 2023

Resin-based friction materials (RBFMs) strengthened by polyether ether ketone (PEEK) fiber were designed and prepared in this study. Specimens incorporating PEEK fiber of 2–8 wt.% were fabricated based on wet granulation, and then the effects of the PEEK fiber content on the mechanical and tribological properties of RBFMs were systematically investigated. The results showed that PEEK fiber can sense the braking temperature and then effectively regulate the comprehensive properties of RBFMs. The specimen incorporating 6 wt.% PEEK fiber obtained the optimal comprehensive performance with a stable friction coefficient (COF), excellent fade resistance and recovery properties, and better wear resistance. The worn surface was inspected using a scanning electron microscope. After the friction–wear test, the specimen with 6 wt.% PEEK fiber presented a number of primary and secondary plateaus and a reduced number of pits with wear debris on the worn surface. The study indicated that PEEK fiber could not only enhance the mechanical and tribological properties of RBFMs at low temperatures because of their high strength and self-lubrication but also adhere to wear debris to reduce abrasive wear at high temperatures; furthermore, the adhered wear debris could form a secondary plateau under normal pressure, which could alleviate abrasion.
Mechanistic Insights into Anion-Induced Electrochromism of Ru(II)-Based Metallo-Supramolecular Polymer
Authors: Fu Xiaofang , Zhang Zhuohui , Cao Zhenhu , Rogachev Alexandr Alexandrovich , Yarmolenko Maxim Anatolievich , Chen Tao , Cao Hongtao , Zhang Hongliang
Published: December 2023

The metallo-supramolecular polymer (MSP) is considered one of the most promising electrodes for electrochromic devices due to its intrinsically stable redox properties. Nevertheless, despite extensive work focusing on improving the electrochromic and electrochemical properties of MSPs, little experimental evidence exists from in-depth investigations on the anion-induced electrochromism of MSPs. Herein, Ru-based metallo-supramolecular polymer (polyRu) films with excellent electrochromic performance were fabricated through a novel electrochemical deposition method, and the electrochromic mechanism was further understood. The polyRu films possess fast reaction kinetics with a short switching time of 4.0 s (colored) and 2.8 s (bleached) and highly reversible redox properties due to the resulting impacts on the capacitive behaviors (containing surface, near-surface and intercalation pseudo-capacitance) of the perchlorate ions in the electrochromic process. Moreover, the electrochromic degradation of the polyRu films is considered to stem from the numerous nanopores in the film induced by ClO4− transport and the exchange of counter anions from Cl− to ClO4−. In addition, a physical model, revealing the transport of conduction ions and the evolution of the structure and properties of the polyRu film during the electrochromic process, is presented. It is observed that the charge balance of Ru3+ and Ru2+, achieved through the adsorption/desorption of ClO4− on the film, provides electrochromic and electrochemical reversibility to the polyRu film under positive/negative bias. Correspondingly, a transformation from polyRu·(Cl−)2n to polyRu·(ClO4−)x(Cl−)2n−x in the polyRu film is induced by a counter anion exchange from Cl− to ClO4−. Revealing the detailed perchlorate ion transfer kinetics and electrochromic mechanism in this film can offer new insights into the application of metallo-supramolecular polymers in electrochromic devices.
Determination of Optimal Technological Parameters for Sorting Wheat Grains in Chambers of Different Constructions
Authors: Vaiciukevičius Edvardas , Jasinskas Algirdas , Jotautienė Eglė , Domeika Rolandas , Gerdauskas Kasparas , Zinkevičienė Raimonda , Lekavičienė Kristina
Published: December 2023

In order to extend grain’s storage time and ensure its quality, it is necessary to sort and clean it. The aim of this study was to justify the rational shape of the sorting chamber and the optimal technological parameters for the sorting of wheat grains in airflow. This study used newly designed grain sorting chambers with constant, widening, and narrowing cross-sections for the airflow sorting of “Skagen” wheat grain. The aerodynamic properties of wheat grains were investigated when moisture was at 14 ± 2.0%. The grain flow rate in the chambers varied from 4 to 12 kg min−1 every 2 kg min−1. In addition, the airflow velocity varied from 8 to 12 m s−1 every 1 m s−1. The tilt angle of the constant cross-section camera was increased to 5°. Experimental studies have determined a terminal airflow velocity of 11.53 m s−1 for wheat grains. At the terminal airflow velocity, the grain flight coefficient was obtained to be about 0.074. These studies showed that the narrowing chamber is preferable for lower grain flow rates compared to the constant cross-section of the chamber. The widening chamber requires a lower airflow velocity to achieve the same performance and quality as the other chambers.
Application of Intelligent Control in Chromatography Separation Process
Authors: Xie Chao-Fan , Zhang Hong , Hwang Rey-Chue
Published: December 2023

Chromatographic separation plays a pivotal role in the manufacturing of chemical products and biopharmaceuticals. This technique exploits differences in distribution between stationary and mobile phases to separate mixtures, impacting final product quality. Simulated moving bed (SMB) technology, recognized for its continuous feed, enhances efficiency and increases production capacity while reducing solvent and water consumption. Despite its complexity in controlling variables like flow rates and valve switching times, traditional control theories fall short. This study introduces an intelligent fuzzy controller resembling an approximate neural network (NN) for SMB control. Simulation results demonstrate the controller’s effectiveness in achieving desirable outcomes for the SMB system.
A Proposed Methodology to Evaluate Machine Learning Models at Near-Upper-Bound Predictive Performance—Some Practical Cases from the Steel Industry
Authors: Carlsson Leo S. , Samuelsson Peter B.
Published: December 2023

The present work aims to answer three essential research questions (RQs) that have previously not been explicitly dealt with in the field of applied machine learning (ML) in steel process engineering. RQ1: How many training data points are needed to create a model with near-upper-bound predictive performance on test data? RQ2: What is the near-upper-bound predictive performance on test data? RQ3: For how long can a model be used before its predictive performance starts to decrease? A methodology to answer these RQs is proposed. The methodology uses a developed sampling algorithm that samples numerous unique training and test datasets. Each sample was used to create one ML model. The predictive performance of the resulting ML models was analyzed using common statistical tools. The proposed methodology was applied to four disparate datasets from the steel industry in order to externally validate the experimental results. It was shown that the proposed methodology can be used to answer each of the three RQs. Furthermore, a few findings that contradict established ML knowledge were also found during the application of the proposed methodology.
Exploring the Link between Smartphone Use, Recorded Violence, and Social Sharing in 80 Case Studies in Italy
Authors: Rizzo Amelia , Princiotta Emanuela , Iuele Giada
Published: December 2023

The increasing prevalence of violence recorded and shared through smartphones in today’s digital age has raised concerns about the underlying reasons driving such behavior. However, the lack of experimental studies and scientific evidence exploring the relationship between smartphone use and acts of violence has hindered our understanding of this phenomenon. To bridge this gap, the present study aimed to investigate the potential link between smartphone usage and the perpetration of violence, specifically focusing on incidents where violent acts were recorded and shared publicly. Given the challenges associated with directly observing such occurrences and the limitations of self-reporting due to social desirability bias, the study adopted a novel approach by analyzing major news outlets. Cross-referencing the most recent cases involving 80 episodes of violence, spanning from 2017 to 2023, accompanied by smartphone-recorded videos, the research aimed to gain insights into the role and outcomes of content dissemination. The findings revealed a concerning trend, indicating a rise in violence perpetrated with the aid of smartphones, where subsequent sharing on social networks and instant messaging platforms contributed to the viral spread of such content. This study provides valuable insights into the connection between smartphone usage, violence, and the sharing of violent content. The implications of these findings highlight the need for further research and the development of tools to detect and address violence-related issues in the digital space. Moreover, it emphasizes the importance of responsible social media usage and collective efforts to curb the spread of violent content and foster a safer online environment.
Increased Yields of the Guanine Oxidative Damage Product Imidazolone Following Exposure to LED Light
Authors: Kawada Taishu , Maehara Moka , Kino Katsuhito
Published: December 2023

Among the bases of DNA, guanine is the most easily oxidized. Imidazolone (Iz) is a guanine oxidative damage, and we sought to generate Iz-containing oligomers. In this paper, we describe the methods and conditions to increase the yield of Iz by employing photooxidation reactions using light-emitting diodes (LEDs) with emission wavelengths of 365 nm and 450 nm. For photooxidation performed with the 450 nm LED source at light intensities of 2.75–275 mW/cm2, peak yields of Iz were 35% at light intensities of 27.5 and 68.8 mW/cm2. For reactions performed with the 365 nm LED source at light intensities of 5.12–512 mW/cm2, the peak yield of Iz was 34% at a light intensity of 51.2 mW/cm2. By varying the irradiation time, the maximum yield of Iz (34–35%) was obtained with irradiation times of 5–20 min using the 450 nm LED source at an intensity of 13.8 mW/cm2. Using the 365 nm LED source at an intensity of 25.6 mW/cm2, the maximum Iz yield obtained was 31% at irradiation times of 2–5 min. Thus, we obtained conditions that can provide an Iz yield of up to 35%.
The Strategy of Interpreting the Daodejing through Confucianism in Park Se-dang’s Sinju Dodeokgyeong
Authors: Seo-Reich Heejung
Published: December 2023

This research study examines Park Se-dang’s Sinju Dodeokgyeong, which was the first complete exegesis of the Daodejing (DDJ) in Korea. This study investigates the theoretical strategies that Park used to interpret the DDJ from a Neo-Confucian perspective and also examines the logical missteps that Park took to force a unity between Neo-Confucianism and Daoism. The core method for interpreting the DDJ that Park utilized in his attempt to assert the compatibility of Neo-Confucianism and Daoism can be summarized as “interpreting Daoism through Neo-Confucian theory”. This research study breaks down Park’s strategy for reinterpreting the DDJ, dividing Park’s argumentation into four parts: (1.) clarifying the historical hereticalization of the DDJ; (2.) identifying the ethics and treasured virtues of Confucianism and Daoism; (3.) the study of the cosmologies of Confucianism and Daoism; and (4.) interpreting Daoist moral ethics through Neo-Confucian cosmological theory. Park Se-dang’s strategy for forcing unity between Neo-Confucianism and Daoism had its limits. Among other things, Park attempted but failed to narrow the gap between Confucian and Daoist ethics and cosmology by converting the concept of "heaven" in the DDJ into a humanized heaven. Eventually, even though Park’s strategy failed, his work inspired other Silhak scholars of Joseon up to the 19th century and had a clear impact on the many subsequent reinterpretations of the DDJ.