bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of sample size on kinetic parameters of roughage and concentrated feeds by a semi-automated in vitro gas production system
Authors:   Universidade Federal de Mato Grosso, Ferro Mariane Moreno , Cabral Luciano da Silva ,   Universidade Federal de Mato Grosso, Barros Livia Vieira de ,   Universidade Federal de Mato Grosso, Araujo Claudio Vieira de ,   Universidade Federal de Mato Grosso, Paula Nelcino Francisco de ,   Universidade Federal de Mato Grosso
Published: April 2022

This study aimed to evaluate the effect of different amounts of incubated samples on the kinetic parameters of in vitro fermentation of roughage and concentrated food used for feeding ruminants. Samples were prepared using 200, 300, 400, and 500 mg of air-dried roughage and concentrated sample, ground to 1 mm, and placed in 120 mL glass flasks. Next, inoculum and McDougal solution were added, and the readings were obtained using a semi-automated pressure transducer up to 96 h after the beginning of the incubations. Gas production of the non-fibrous fraction increased linearly (P < 0.05) for sugarcane, Marandu grass silage, corn silage, dried corn distillers’ grains with solubles, dried brewer’s yeast, bean residue, wet brewer’s grains, sunflower meal, and Jatropha meal; quadratically (P < 0.05) for Napier grass silage and cottonseed meal; and cubically (P < 0.05) for castor meal and soybean meal. The degradation rate of the non-fibrous fraction reduced linearly (P < 0.05) for sugarcane, Napier grass silage, and castor meal; quadratically (P < 0.05) for Marandu grass silage; and cubically (P < 0.05) for corn silage, soybean meal, dried corn distillers’ grains with solubles, bean residue, and cottonseed meal. Gas production of the fibrous fraction increased linearly (P < 0.05) for Napier grass silage, Marandu grass silage, corn silage, dried corn distillers’ grains with solubles, bean residue, wet brewer’s grain, cottonseed meal, and sunflower meal; quadratically ( < 0.05) for Jatropha meal; and cubically (P < 0.05) for sugarcane, castor meal, and soybean meal. The degradation rate of the fibrous fraction increased linearly (P < 0.05) for Napier grass silage, dried corn distillers’ grains with solubles, dried brewer’s yeast, wet brewer’s grains; quadratically (P < 0.05) for corn silage and castor meal; and cubically (P < 0.05) for sugarcane, Marandu grass silage, and bean residue. The lag time reduced linearly (P < 0.05) for castor meal and dried corn distillers’ grains with solubles; quadratically (P < 0.05) for Napier grass silage; and cubically (P < 0.05) for sugarcane, Marandu grass silage, corn silage, soybean meal, bean residue, cottonseed meal, sunflower meal, and Jatropha meal. Thus, our findings suggest that the kinetic parameters of in vitro fermentation were affected as a function of the amount of incubated sample.
Adaptability of sheep to three salinity levels in different environments
Authors:   Universidade Federal de Campina Grande, Furtado Dermeval Araújo , Carvalho Junior Sebastião Benicio ,   Universidade Federal de Campina Grande, Lopes Neto José Pinheiro ,   Universidade Federal de Campina Grande, Souza Bonifácio Benício de ,   Universidade Federal de Campina Grande, Dantas Nayanne Lopes Batista ,   Universidade Federal de Campina Grande
Published: April 2022

The objective of this study is to evaluate physiological parameters and the adaptability of Santa Inês sheep to two climatic conditions 25ºC (within the thermal comfort zone [TCZ) and 32ºC (above the TCZ) and three salinity levels (2.0, 4.0, and 8.0 dS m-1). The study was developed in a climatic chamber using 36 uncastrated male sheep. The experimental design was completely randomized with a 2 ´ 3 factorial scheme composed of two temperatures, three water salinity levels, and six repetitions. The physiological variables were not affected by the salinity levels. The rectal and skin temperature and the heart and respiratory rate were higher at 32 °C. However, the rectal temperature was within the normal range for the species. The consumption of water with different salt concentrations did not affect the adaptive responses of the animals and could be an alternative water source for sheep in regions where water has a high salinity level. The respiratory rate of sheep exposed to 32 °C increased to eliminate body heat, and exposure to 25 °C provided greater thermal comfort for the animals.
Evaluation and characterization of triticale silage (x. Triticosecale wittmack) to replace Sorghum bicolor (L.) Moench (S. vulgare Pers.) silage as feed for beef cattle
Authors:   Universidade Estadual de Londrina, Henz Éderson Luis , Silva Leandro das Dores Ferreira da ,   Universidade Estadual de Londrina, Bumbieris Junior Valter Harry ,   Universidade Estadual de Londrina, Massaro Junior Fernando Luiz ,   Universidade Estadual de Londrina, Zanin Ediane ,   Universidade Estadual de Londrina, Arruda Maria Carolina Gonçalves de ,   Universidade Estadual de Londrina
Published: April 2022

The objective of this study was to evaluate the nutritional quality of silages containing 0, 25, 50, 75 and 100% triticale replacing sorghum silage feed for beef cattle. It was carried out fractionation of carbohydrates and proteins, and the parameters of ruminal degradation kinetics estimated by semiautomatic in vitro method of cumulative gas production of the silages. The experimental design completely randomized with five treatments and four replicates. Sorghum silage has less non-fibrous carbohydrates, and its substitution may explain the linear increase in the composition of fractions A and B1. In general, the proportions of fractions “A” and “B1 + B2” demonstrated that as sorghum replaced by triticale, the abundance of high availability proteins increased, as did the abundance of rapidly degradable proteins in the rumen. The final volume of gases produced by the fermentation of non-fibrous carbohydrates presented a negative linear relationship with increased triticale silage (P = 0.0001), while the degradation rates presented a positive linear relationship (P = 0.0001). Thus, there was rapid release of energy and nitrogen, which may meet the needs of microorganisms. Therefore, triticale silage can be substituted for sorghum silage in cattle feed as an excellent source of protein and energy without altering nutritional parameters.
Serological survey of porcine circovirus 2 antibodies in Northeastern Brazil by immunoperoxidase monolayer assay
Authors:   Secretaria de Saúde do Recife, Duque Priscila Rocha , Silva Antonio Samuel Alves da ,   Universidade Federal Rural de Pernambuco, Barros Júnior Marconi Rego ,   Universidade Federal Rural de Pernambuco, Sergio Andreza Maria Tenório ,   Universidade Federal Rural de Pernambuco, Barbosa Clara Nilce ,   Universidade Federal Rural de Pernambuco
Published: April 2022

Porcine circovirus 2 (PCV2) specific antibodies were evaluated in pigs at 15 to 25 weeks of age by immunoperoxidase monolayer assay. Serum samples (n = 575) were obtained from 16 commercial farms located in six states of northeastern Brazil, including two in Ceará (n = 85), three in Rio Grande do Norte (n = 92), two in Paraíba (n = 49), three in Pernambuco (n = 107), two in Alagoas (n = 39), and four in Bahia (n = 203). Out of the 575 evaluated sera, 470 (81.74%) were reagents with antibody titers ranging from 20 to 5,120. All farms presented reactive animals. There was a statistically significant difference in reactivity proportions between the states of Bahia and Ceará (p < 0.05) and between Bahia and Rio Grande do Norte (p < 0.05). This study provides the first serological investigation for PCV2 in pigs from commercial farms in Northeastern Brazil.
Evaluation of resistance against Streptococcus agalactiae in four farmed strains of Nile tilapia (Oreochromis niloticus)
Authors:   Universidade Federal de Lavras, Mian Gláucia Frasnelli , Roncancio Charles Oswaldo ,   Fundación Universitaria San Martín, Silva Maria Cristina de Souza ,   Universidade Federal de Lavras, Ferreira Amanda Carvalho Rosado ,   Universidade Federal de Lavras, Custódio Dircéia Aparecida da Costa ,   Universidade Federal de Lavras, Souza Victor Hugo Silva ,   Universidade Federal de Lavras, Costa Geraldo Márcio da ,   Universidade Federal de Lavras
Published: April 2022

Nile tilapia (Oreochromis niloticus) has always been considered more resistant to diseases compared to other fish species. However, in recent decades, this fish species has also been susceptible to many bacterial, fungal, parasitic, and nutritional diseases. In Brazil, streptococcosis is one of the main diseases that affect tilapia and has caused many economic losses. A resistance test was conducted with 93 tilapia of four varieties (UFLA strain and three commercial varieties: SC1, SC2, and SC3) to evaluate resistance to experimental inoculation with Streptococcus agalactiae. Each variety was distributed in five aquariums, with nine specimens of the same variety. Among the five aquariums, four housed fish were intraperitoneally inoculated with 107 CFU/fish of S. agalactiae and one aquarium harbored fish from the control group, which received 0.1 mL of sterile BHI (Brain Heart Infusion). Significant differences were observed in the survival rates accumulated at the end of the experiment (day 15), with values of 22, 36, 36, and 15% for SC1, SC2, SC3, and UFLA, respectively. The UFLA strain presented lower survival than other strains, which did not differ among the 15 days of challenge. These results demonstrate that there are animals with a better response to the resistance test than others.
Pengaruh Model Pembelajaran Sinektik dalam Mengembangkan Kreativitas Peserta Didik
Authors: Pramusinta Yulia , Rifanah Farah Destria
Published: April 2022

To realize the creative Human resources could be done through education or structured learning by implementing the model of synectic learning which was an important demands. The model of learning had syntax that could stimulate the students' creativities. The research as one of method in examining the difference result of learning creativities between the synectic model and the conventional learning model. The research used experiment test. The sample collection used cluster random sampling about 2 classes learning of Indonesian Language, when the first was controll class that learned the conventional method and the other class was experiment class that learned the synectic learning model. And the research analysis used the independent sample t-test. The research showed that the were influences between synectic learning model toward the students' creativities about the Indonesian Language material.
Meningkatkan Kemampuan Membaca dan Menulis Berbasis Indigenousasi dengan Media Flash Card
Authors: Safa’ah Alfiatus , Rimadhani M Nuria
Published: April 2022

Reading and writing are basic skills that humans must have because these competencies help humans in communicating and carrying out aspects of life. As learners, reading and writing competencies need to be taught as early as possible in preparation for receiving various lessons at school. It can be assumed that students who have not mastered reading and writing skills will not be able to follow the course of learning smoothly. As a result, the learning process will be hampered and learning objectives cannot be achieved. One of the causes of the low ability to read and write in elementary school students is influenced by intelligence factors and the learning process received by students. Indigenization aims to re-express authenticity which is usually native to get to know the local culture where students come from. This kind of cultural theme needs to be introduced to students in learning activities so that students can appreciate local wisdom. At the initial level of elementary school, training on reading and writing skills with a special theme, in this case culture, must be taught regularly, structured and interesting. Therefore, the use of learning media should be considered in developing reading and writing competence. Flash card is one of the effective media to help improve reading and writing skills, especially at the early elementary school level. Card-shaped media with this visual type have pictures and descriptions that can make students more interested in participating in learning. The application of Flash card media in the learning process is accompanied by cooperative strategies so that its use is more effective and can make students more actively involved in learning. With fun activities in the classroom, the goal of improving the reading and writing skills of grade I (one) students will be achieved.
Penggunaan Media Realia dalam Meningkatkan Pengalaman Belajar Siswa
Authors: Ibad Taqwa Nur , Sarifah Maisyatus
Published: April 2022

Education is an effort to foster and spread one's personality, both spiritually and physically. Learning experiences are several student activities carried out to obtain new information and competencies in accordance with the objectives to be achieved. Realia media are tangible objects that are often encountered by students and are easily found in daily activities that can be brought into learning situations or become media for teaching materials for students. These real objects can be used as realia media and these objects must be original and intact in form and also the actual size so that they can be recognized by students properly. Based on the above understanding, it is known that the use of the right realia media will improve the student's learning experience of the material to be studied. The use of realia media in thematic learning, especially in grade 4 MI Nurul Islam Sukosari. And the problem I encountered was that the media used by grade 4 teachers had not yet optimized the long-term learning objectives. This happens because the use of media is monotonous so that the process of learning activities is less memorable for students so that students are less experienced. Therefore, to improve the student learning experience, the researcher chose to raise the title "Use of Realia Media in Improving Student Learning Experience in Grade 4 Thematic Learning".
Analisis Multivarian Terhadap Efektivitas Pembelajaran Daring pada Siswa di Sekolah Dasar Kelas Atas
Authors: Adawiyah Robiatul , Hasanah Uswatun , Firdausi Aulia Kaffah , Lailiyah Siti
Published: April 2022

Online learning has its own challenges for teachers in order to achieve learning outcomes, especially in seeking children's character education as stated in the goals of character education. When learning is carried out online during the pandemic or face-to-face in this new normal era, there will certainly be differences in the implementation of activities, giving rise to various obstacles, especially related to the character of students' discipline and interest in learning. This study was to determine the effectiveness of online learning on upper grade elementary school students. This study also focuses on the character of discipline and interest in student learning in the upper class. This study uses the multivariate analysis technique MANOVA (Multivariate Analysis of Variance). The results of this study indicate that there is a significant difference in the character of students' discipline and interest during online learning and learning in the new normal era with a significance of 0.003 <0.05, which means that learning carried out in the new normal era is more effective than the character of discipline and interest. students during online learning during the covid-19 pandemic. This research is still not able to know the results of the conditions of students specifically so that it is still very necessary for materials and considerations for further research.
Pengaruh Model Pembelajaran Inquiry Terbimbing Terhadap Hasil Belajar Tematik
Authors: Aryanto Sherif Juniar , Shofiyullah Muhammad
Published: April 2022

Learning is a students effort through interaction with the environment to change their behavior. In learning implementation, teachers are required to be able to understand and develop existing learning models, because the use of learning models can stimulate a learning process so that it can motivate students in their learning process, it can make students easier to understand lessons quickly, accurately, and boringless. One of the learning models that can be implemented in the scope of education is the guided inquiry learning model. This study aims to determine the effect of the guided inquiry learning model on thematic learning outcomes of fourth grade students of MI Nurul Huda Sawahan. Based on the results obtained that there is a significant difference in the fourth grade students of MI Nurul Huda, initially many students obtained learning outcomes below the minimum completeness criteria which are originally the pre-test average of 65.5% and increased by 13.5% on the post test value that is equal to 79%. The Gain Score results obtained show a Mean of 77%, which means that this guided inquiry model is effective to use, it is known that the results of the hypothesis analysis carried out are sig 0.000 so <0.005
Kesiapan Mahasiswa Lembaga Pendidikan dan Tenaga Kependidikan (LPTK) di Lombok Menjadi Guru MI/SD
Authors: Munir Muhammad , Alwan Muhammad , Husairi Husairi
Published: April 2022

The purpose of this study is to describe the readiness of higher education LPTK students in Lombok to become professional MI/SD teacher candidates in terms of knowledge, practice, and attitude, as well as to describe the dominant factors that hinder the readiness to become professional MI/SD teacher candidates. This type of research is quantitative survey method. Instruments for collecting test data, questionnaires, and observation sheets. This study aims to obtain an overview of the characteristics of the population related to the readiness of students to become professional MI/SD teacher candidates. The population in this research is Mataram State Islamic University and Mataram University. The sampling technique used is area sampling. The results of this study indicate in terms of knowledge about students and how to learn the results of the analysis show an average value of 25.74 in the high category. This shows that students are ready to become MI/D professional teachers. While the questionnaire on the ability to carry out learning when viewed from the average value, which is 136.24 in the high category. In the aspect of perception of being an MI/SD teacher, the average score is 103.60 with the agree category. Furthermore, on the confidence data to become a professional MI/SD teacher, the data analysis results obtained an average of 140.04 with the agree category
The Students’ Responses of Video Recording and E-Sorogan Learning Methods to Improve Pronunciation
Authors: Saputri Tiyas , Afandi Mujad Didien , Mushaf Anharu Minasalim
Published: April 2022

Mispronunciation leads to misunderstanding because the inaccuracy in pronouncing words may distort the interpretation of ideas in communication. However, using video recording and e-Sorogan learning methods can be the solution to improve students’ pronunciation, especially the ASD and ADHD student. This study purposes to find out the students' responses to the use of these methods to the 39 elementary students, including a student with ASD and ADHD. This study used descriptive qualitative. The collected data were from the questionnaire survey. Next, the data collected from the questionnaire survey was analyzed descriptive qualitatively through the data in the table and based on empirical data. The results indicated that the students' responses to these methods were positive. It can be proved with the data presented in the table. To conclude, the students agree and even strongly agree with the use of these methods to improve their pronunciation although only a few of them disagree with that. Most of them like these methods because they were appropriate, effective, interesting, useful, and successful to improve their pronunciation. Even, they are able to do self-evaluation and correct the mispronunciation from the video made.
Penggunaan Media Diaorama dalam Meningkatkan Kreatifitas Siswa
Authors: Ibad Taqwa Nur , SH Nurul Hidayah
Published: April 2022

Media diorama salah satu media pembelajaran yang digunakan untuk merangsang ide siswa untuk lebih kreatif dalam proses pembelajaran. Media diaorama menirukan benda asli menggunakan benda tiruan berbentuk tiga dimensi berukuran kecil, digunakan untuk menggambarkan situasi pemandangan yang sebenarnya. Tujuan penelitian ini untuk meningkatkan kreatifitas siswa melalui penerapan media diorama pada siswa kelas enam Madrasah Ibtidaiyah Barokatul Muhid Lumajang dengan menggunakan jenis penelitian penelitian deskriptif dengan pendekatan kualitatif, teknik di dalam penerapan media diorama ini salah satunya participant observation yaitu merupakan teknik pengumpulan suatu data dimana para peneliti secara langsung terlibat atau merasakan dari kehidupan subjek yang ditelitinya. Hasil penelitian menunjukan rancangan media diorama pada pembelajaran tematik perkembangbiakan generatif dan vegetatif dimana siswa membuat konsep sendiri dalam membedakan jenis perkembangbiakan pada sebuah media gambar berbentuk tiga dimensi. jadi siswa sendiri yang sudah dibagi dalam beberapa kelompok diharapkan mampu memaparkan perbedaan perkembang biakan vegeatif dan generative tumbuhan dan hewan dalam bentuk materi dengan penerapan pembelajaran menggunakan media diaroma.
The Validity and Reliability of the Group Regulation Scale Turkish Form: A Study with the Rasch Model
Authors: YILDIZ DURAK Hatice , AKSU Beyza
Published: April 2022

In this study, the group regulation scale was adapted to Turkish and validity was checked using Rasch Model. The original scale was created by adapting the form developed by Papamitsiou and Economides (2019) by Lai (2021). The scale used in this study was adapted into Turkish by the researchers with permission from Lai (2021). The original scale consists of 12 items scored on a five-point Likert type scale and 4 sub-dimensions (effort regulation, goal expectancy, help seeking and time management). This research was carried out on 170 university students based on voluntary participation using purposive sampling method. Rasch analysis was used for analyzing data in order to examine validity and reliability of the scores. According to the Rasch analysis results, it was concluded that the group regulation scale is a unidimensional measure of group regulation among university students.
ANALISIS IMPLEMENTASI TANGGUNG JAWAB SOSIAL BERBASIS STAKEHOLDER PADA PERUSAHAAN PERKEBUNAN KELAPA SAWIT
Authors: Dhamayanti Sylvia Kartika
Published: April 2022

Penelitian ini menganalisis penerapan Corporate Social Responsibility (CSR) pada perusahaan keluarga. Perusahaan melaksanakan CSR untuk memenuhi tanggung jawabnya dalam menyelaraskan nilai-nilai bisnis yang terkait dengan kebutuhan dan harapan pemilik dan pemangku kepentingan lainnya. CSR diyakini dapat menghasilkan dan memelihara hubungan yang menguntungkan baik dengan pihak-pihak yang terlibat langsung maupun tidak langsung dalam aktivitas perusahaan. Penelitian ini merupakan studi kualitatif yang mencoba menjabarkan implementasi CSR pada perusahaan perkebunan kelapa sawit milik keluarga yang berlokasi di Kalimantan Selatan. Data diperoleh dari laporan tahunan perusahaan dan wawancara dengan informan kunci. Hasil penelitian menunjukkan bahwa perusahaan telah menjalankan CSR sebagai perwujudan dari perilaku legal, etis, dan tanggung jawab sosial. Secara tidak langsung, CSR merupakan bagian dari pemasaran menyeluruh yang pendukung keberlangsungan dan perkembangan aktivitas bisnis.
ANALISIS PERLAKUAN AKUNTANSI ASET BIOLOGIS PADA PT DHARMA SATYA NUSANTARA TBK BERDASARKAN PSAK 69 AGRIKULTUR
Authors: Kuncara Tommy
Published: April 2022

Aset biologis merupakan suatu sumber daya berupa makhluk hidup (hewan atau tumbuhan) yang dipelihara dan dikelola oleh entitas dari peristiwa masa lalu agar memperoleh manfaat ekonomi/kekayaan di masa mendatang. Penelitian ini bertujuan untuk mengetahui bagaimana perlakuan akuntansi aset biologis pada PT Dharma Satya Nusantara Tbk, serta untuk mengetahui bagaimana perbandingan perlakuan akuntansi aset biologis pada PT Dharma Satya Nusantara dengan PSAK 69 Agrikultur.  Metode penelitian ini menggunakan metode deskriptif komparatif, yaitu berupa metode yang membandingkan antara dua variabel dalam suatu aspek yang diteliti, serta penelitian ini menggunakan cara pembahasan dengan pola pikir induktif, yaitu diawali dengan membahas permasalahan khusus untuk memperoleh suatu kesimpulan umum.  Hasil penelitian menunjukkan bahwa PT Dharma Satya Nusantara Tbk yang bergerak dalam bidang perkebunan mengakui aset biologis berupa pohon dalam hutan kayu dan produk panen dari tanaman produktif yaitu berupa TBS, dan pada tanaman produktif ini tanaman diklasifikasikan menjadi tanaman belum menghasilkan dan tanaman menghasilkan. Aset biologis diukur menggunakan nilai wajar dan disajikan dalam aset lancar pada aset biologis berupa TBS, dan aset tidak lancar pada aset biologis berupa pohon dalam hutan kayu dan tanaman produktif. Secara umum, PT Dharma Satya Nusantara sudah menerapkan PSAK 69, hanya saja terdapat perbedaan pada penyajiannya
PERANCANGAN SISTEM INFORMASI ABSENSI PEGAWAI PADA PERUSAHAAN MANUFAKTUR PRE-SERVER BERBASIS DESKTOP
Authors: Purwandari Nuraini
Published: April 2022

Perusahaan Pre-server merupakan suatu perusahaan manufaktur yang masih menggunakan sistem absensi secara manual, harus membutuhkan sistem yang baik sehingga dalam proses pencatatan kehadiran karyawan dapat lebih lancar dan terintegrasi dalam suatu sistem jaringan kerja. Tujuan dari penelitian ini adalah merancang sistem informasi absensi pegawai agar memberikan kemudahan kepada staf bagian HRD (Human Resource Department) yang dalam hal ini berperan sebagai orang yang berwenang untuk melakukan proses absensi. Dan juga memberikan kemudahan kepada pihak yang bertanggung jawab untuk mengolah data absensi. Aplikasi ini dirancang dengan metode pendekatan sistem berorientasi objek dan metode pengembangan sistem SDLC (System Development Life Cycle) yaitu model waterfall. Aplikasi ini dibangun menggunakan bahasa pemrograman Java Spring Framework dan MySQL sebagai media penyimpanan data. Hasil penelitian ini adalah sebuah rancang bangun aplikasi absensi pegawai yang dapat membantu pengolahan data yang ada dan mempercepat proses absensi karyawan
FAKTOR-FAKTOR PENGARUH PENGGUNAAN MOBILE BANKING
Authors: Wardani Deni
Published: April 2022

Penelitian ini bertujuan untuk menguji pengaruh persepsi kegunaan, persepsi kemudahan penggunaan, kepercayaan terhadap sikap penggunaan, dan kebiasaan penggunaan mobile   banking nasabah bank pemerintah. Penelitian dilakukan menggunakan kuesioner online kepada nasabah pengguna mobile banking bank pemerintah di Jakarta, Tangerang, Depok dan Bekasi. Penelitian ini menggunakan deskriptif kuantitatif. Metode pengambilan sampel berupa purposive sampling. Metode analisis data berupa analisis path menggunakan PLS-SEM. Hasil penelitian ini menunjukkan bahwa persepsi kegunaan, persepsi kemudahan penggunaan, dan kepercayaan berpengaruh signifikan terhadap sikap penggunaan, dan sikap penggunaan berpengaruh signifikan terhadap kebiasaan penggunaan mobile banking bank pemerintah
Application of partial Cytochrome b and 12S rRNA Genes for Mammalian species identification
Authors:   Centre for Wildlife Forensic Sciences, Advanced Institute for Wildlife Conservation,Tamil Nadu Forest Department,Chennai-600048,India, Chandramouli Vaishnavi , Anbazhagan Pradeep ,   Centre for Wildlife Forensic Sciences, Advanced Institute for Wildlife Conservation,Tamil Nadu Forest Department,Chennai-600048,India, Kumari Vasantha ,   Centre for Wildlife Forensic Sciences, Advanced Institute for Wildlife Conservation,Tamil Nadu Forest Department,Chennai-600048,India, Rangasamy Kanchana ,   Centre for Wildlife Forensic Sciences, Advanced Institute for Wildlife Conservation,Tamil Nadu Forest Department,Chennai-600048,India, Jana Debasis ,   Centre for Wildlife Forensic Sciences, Advanced Institute for Wildlife Conservation,Tamil Nadu Forest Department,Chennai-600048,India
Published: April 2022

Determination of species from wildlife specimens is one of the prime goals of forensic laboratories analyzing samples involved in wildlife crimes. The conviction rate in wildlife crimes is often low due to a lack of evidential support in identification of the species involved in crime. When species identification by application of morphological techniques fails due to sample autolysis or lack of anatomical markers in wildlife specimens, DNA analysis provides the vital, foolproof cue. Sequences generated from mitochondrial DNA regions are used to determine the species by matching against a known reference sequence, either using the global GenBank database or using references created locally. The present study employed species identification by DNA analysis using wildlife samples received from the Forest Department across Tamil Nadu, India. The study demonstrates the utility of partial Cytb and 12S rRNAgenes for determination of species from 18 samples without recognizable morphological features, including tissue, hair and blood. The species of the collected specimens were correctly identified by sequence similarity search with 99 to 100% match and taxonomic classification using phylogenetic tree reconstruction. Of the 18 samples analyzed, accurate species identification using 12S rRNA gene was possible for all the samples, while Cytbgene-based identification was successful for 16 samples. Sequences generated from the study could also serve as a local genetic databank for the State Forest Department to match against sequences from wildlife forensic samples referred to the Institute for ascertaining species involved in wildlife trade. The sequences will also be useful to bridge gaps in genetic data on species native to Tamil Nadu.
Association between circulating MicroRNAs (hsa-miR-92a-1* and hsa-miR-454) and multiple sclerosis phenotypes and activity statu
Authors:   Department of Clinical Pathology & Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Moharram Amira , Amer Hanaa ,   Department of Clinical Pathology & Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Bakr Salwa ,   Department of Clinical Pathology & Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Fouad Nermeen ,   Department of Clinical Pathology & Immunology, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Zamzam Dina ,   Department of Neurology, Faculty of Medicine, Ain Shams University, Cairo, Egypt, M Rushdy
Published: April 2022

Efficient diagnosis of multiple sclerosis (MS) disease along with early prediction of its progression will ultimately lead to better management, control of complications and improvement of therapeutic outcomes and patient’s well-being. Blood based biomarkers like circulating microRNAs represent a non- invasive, fast, and easily measured markers with a promising potential. This work intended to assess the relative expression of circulating hsa-miR-454 and hsa-miR-92a-1* as a diagnostic and prognostic tool among Egyptian MS patients in terms of correlation to disease type and severity. hsa-miR-454 and hsa-miR-92a-1* relative expression was measured in the plasma of 31 MS patients, relapsing remitting MS (RRMS, n=21) and progressive MS (PMS, n=10) and 20 age and sex matched normal controls by using reverse transcription followed by real time PCR. Disease severity assessment was done in the form of patient expanded disability status scale (EDSS) evaluation. Relative expression of hsa-miR-454 and hsa-miR-92a-1* did not show a statistically significant difference between MS cases and controls. However, hsa-miR-454 was significantly higher among RRMS patients in comparison to PMS patients (P = 0.04). Additionally, both markers showed a statistically significant upregulation among patients in disease exacerbation in comparison to patients in remission (P = <0.01) and both showed a negative correlation with EDSS. In conclusion, microRNAs may represent potential valuable non-invasive biomarkers for assessment of MS type (RRMS vs PMS), as well as for prediction of disease activity and severity in MS patients.
Assessment of angiotensin converting enzyme gene polymorphism in patients with psoriasis
Authors:   Dermatology, Venerology & Andrology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Ashry Nagwa , Attia Fadia ,   Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Ahmed Amal ,   Dermatology, Venerology & Andrology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Nada Hesham ,   Dermatology, Venerology & Andrology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt., Maaty Ahmed ,   Physical Medicine, Rheumatology, & Rehabilitation, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Published: April 2022

Psoriatic patients had diversity of clinical presentations and complications. Psoriasis can have significant interference with the patient's quality of life, recovery, and outcome. Some evidences suggest that the angiotensin converting enzyme (ACE) is present in the skin of psoriatic patients. This study intended to assess the patterns of ACE insertion/deletion (ACE ID) polymorphism and the levels of serum ACE among psoriatic patients in comparison to normal controls. The study included two groups: 20 patients with psoriasis and 20 apparently healthy adults with negative family history of psoriasis as a control group. Psoriasis area and severity index (PASI) was used to measure of severity of psoriasis. In both groups, ACE ID gene polymorphism was assessed by quantitative real-time polymerase reaction and serum ACE levels was evaluated using an enzyme-linked immunosorbent assay. ACE ID genotype was significantly higher among the psoriatic group in comparison to the control group (40.0% versus 15.0%, respectively, p=0.016). D allele was significantly higher among the psoriatic group than the control group (25.0% versus 7.5%, respectively, p=0.034). ACE ID genotype carried significantly higher risk in psoriatic group versus control group (OR=3.8). The D allele carried higher risk in psoriatic group versus control group (OR=4.1). ACE serum levels were significantly higher among the psoriatic group compared to the control group (87.4±7.03 versus 2.3±0.7, respectively; p<0.001). We concluded that ACE ID gene polymorphism may be considered as a risk factor for developing psoriasis.
Serum clusterin as a promising diagnostic and prognostic marker for hepatocellular carcinoma after locoregional treatment
Authors:   Gastroenterology & Hepatology Unit, Department of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Rasmy Hany , Mohammed Hatem ,   Gastroenterology & Hepatology Unit, Department of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Mohammed Eslam ,   Gastroenterology & Hepatology Unit, Department of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Ahmed Aml ,   Gastroenterology & Hepatology Unit, Department of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Isaac Amira ,   Gastroenterology & Hepatology Unit, Department of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Published: April 2022

Hepatocellular carcinoma (HCC) is one of the most common aggressive tumors, with a rising prevalence in Egypt. Clusterin is a secretory heterodimeric glycoprotein linked to cancer development and progression. This study was conducted to evaluate the diagnostic and prognostic role of serum clusterin as a possible biomarker of HCC and correlate its level with the mRECIST scoring system. This study included 45 patients with liver cirrhosis and HCC eligible for locoregional treatment and 20 patients with liver cirrhosis without HCC as controls. All patients underwent standard laboratory tests and abdominal ultrasound. For HCC patients, a triphasic CT scan, alpha-fetoprotein (AFP), and clusterin levels were measured at baseline and one month after intervention. HCC patients had a substantially higher baseline clusterin level than cirrhotic patients (122.291 ± 61.898 vs. 74.015 ± 41.571, P = 0.002). Five patients in the HCC group were not eligible for intervention because they had evidence of portal vein invasion. At one month follow-up after HCC treatment, serum clusterin levels declined significantly from baseline (from 122.291 ± 61.898 to 81.125 ± 62.321, P = <0.001). According to the mRECIST scoring, baseline clusterin levels were significantly higher among patients with progressive disease than those with partial response than those with complete response (180.722 ± 55.908, 161.310 ± 56.339, 84.810± 41.389, respectively, overall P = <0.001). Clusterin was a useful marker in detecting HCC with 73.33% sensitivity and 75% specificity at a cutoff of ≥ 86.6 mg/L, and it also had 95.24% sensitivity and 77.78% specificity in detecting tumor progression at a cutoff of ≥ 146.6 mg/L, according to the mRECIST scoring system. In conclusion, clusterin may be a helpful diagnostic and prognostic marker for HCC after locoregional treatment, as its baseline level is useful in predicting response and progression of HCC in correlation with the mRECIST scoring system.
TFRS 9 ve BDDK DÜZENLEMELERİNİN BANKALARIN KREDİ KARŞILIKLIĞI AYIRMADAKİ ETKİLERİNİN ANALİZİ
Authors: ERBEN YAVUZ Asuman , HAZAR Adalet , BABUŞCU Şenol
Published: April 2022

1 Ocak 2018 tarihiyle yürürlüğe giren TFRS 9 kapsadığı yeni uygulamalar özellikle bankalar için önem teşkil etmekle beraber yatırımcılar, analistler, düzenleyici ve denetçiler açısından da gündem konulardan biri olmuştur. TFRS 9 uygulamasının yürürlüğe girmesiyle beraber “beklenen kredi zararı” ve “ömür boyu beklenen kredi zararı” tanımları değiştirilmiş ayrıca finansal durum tablolarında da gösterilme şekilleri yeniden düzenlenmiştir. Bu çalışmada TFRS 9 kapsamında bankaların finansal tablolarında oluşan değişikliklerden kredi sınıflandırmaları ve bunlar için ayrılacak karşılıkların, TFRS 9’a geçmeden önce ve sonrasında meydana gelen değişiklikler incelenmiştir. Çalışmanın sonucunda bankaların donuk alacakları için ayırdıkları karşılıkların TFRS 9’un uygulamaya geçildiği yılda önemli ölçüde azaldığı ancak sonraki yıllarda tekrar yükseldiği, standart ve yakın izlemede kredi gruplarına ait karşılıklarda da artışın yüksek olduğu görülmüştür.
Public Handling of Protective Masks from Use to Disposal and Recycling Options to New Products
Authors: Remic Katarina , Erjavec Alen , Volmajer Valh Julija , Šterman Sonja
Published: April 2022

A study was conducted on the waste of disposable surgical masks and their problematic impact on the environment. The studies examined have shown the negative effects on the environment that are likely to occur and those that have already occurred. In this article, society's relationship to the potential recycling of disposable surgical masks is considered and projected onto the possibilities of the cradle-to-cradle design approach. The development of a product from recycled surgical masks is driven by two different surveys. The first focuses on wear and disposal habits, and the second on the relationship to recycling. As a result, the flooring was developed with thermally treated recycled surgical masks replacing the filler layer. The goal of the product design was to improve the long-term life cycle analysis of a waste surgical mask.
Gingival involvement of oral non-tumoral mucosal diseases
Authors: Tofan Elena C. , Parlatescu Ioanina ,   Carol Davila University of Medicine and Pharmacy, Coculescu Elena ,   Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, Tovaru Serban ,   Carol Davila University of Medicine and Pharmacy, Nicolae Carmen ,   University of Agronomic Sciences and Veterinary Medicine of Bucharest, Coculescu Bogdan I. ,   "Cantacuzino" National Medico-Military Institute for Research and Development, "Carol Davila" University of Medicine and Pharmacy, "Carol Davila" University of Medicine and Pharmacy, and Military Medical Institute, "Nicolae Titulescu " University, Center for Military Medical Scientific Research, Center for Preventive Medicine of the Ministry of National Defence, Titu Maiorescu University, University of Bucharest
Published: April 2022

Gingival conditions are frequently encountered in dental clinical practice and they can raise diagnosis and treatment problems. The diagnostic is variable from normal conditions to autoimmune diseases or even malignancy. Some of them cause disturbances such as pain, burning sensation and have an impact on patient’s quality of life.
Emerging concepts regarding the molecular profile of breast carcinoma: one-year experience in a University Center
Authors: Voinea Oana C. , Sajin Maria , Dumitru Adrian V. ,   Universitatea de Medicina si Farmacie Carol Davila Facultatea de Medicina, Universitatea din Bucuresti Facultatea de Biologie, University Emercency Hospital Bucharest, Patrascu Oana M. , Georgescu Tiberiu A. , Cirstoiu Monica M. , Jinga Dan C. , Nica Adriana E.
Published: April 2022

Breast carcinoma represents the leading cause of oncologic mortality for women. Due to therapeutic and diagnostic advances, the mortality and morbidity in the last decade declined, but breast cancer still has a great impact on quality of life and also on medical service cost. In the light of these facts, an integrated approach, considering histopathology features, molecular profiles and corroboration with clinical and imagistic data is necessary. A descriptive retrospective one-year study analyzed the breast cancer heterogeneity in 121 cases registered in the Pathology Department of the University Emergency Hospital in Bucharest, Romania. Our purpose was to evaluate histopathological, immunohistochemically, clinical and imagistic aspects of breast cancer considering the current molecular classification (Luminal A, Luminal B, HER2 enriched and triple negative/basal like). Our assay revealed that most prevalent histotype was NST (no special type) followed by invasive lobular carcinoma. Considering the molecular pattern, the most common was luminal B. Triple negative basal like and HER 2-enriched were correlated with an aggressive morphologic pattern and lymph-nodes positivity. Considering the imagistic acquisitions, mammography proved to be the most accurate technique for measuring the dimension of NST. In conclusion, breast carcinoma is a heterogeneous disease that needs an integrated approach and personalized treatment based on the histopathologic and molecular features. Considering the great number of advanced stages as diagnosis, a national screening program for breast cancer is imperiously needed.
Analysis of risk factors for antipsychotic-resistant schizophrenia in young patients – A retrospective analysis
Authors:   Dr. Carol Davila University of Medicine and Pharmacy, Dr.Carol Davila University Emergency Central Military Hospital, Universitatea Titu Maiorescu, University of Bucharest, Vasiliu Octavian , Vasile Daniel , Fainarea Andreea F. , Patrascu Mihaela C. , Morariu Elena A. , Manolache Raluca , Alexandru Iulia , Androne Flavius T.
Published: April 2022

Treatment resistant schizophrenia (TRS) is a severely disabling disorder, which decreases dramatically the quality of life and overall functionality, while it increases the rate of hospital admissions and overall healthcare costs. The main objective of this research was to evaluate the risk factors for TRS in a group of patients based on a retrospective analysis. The secondary objective was to design an algorithm for initial evaluation in patients with schizophrenia, in order to detect the candidates at risk for developing TRS. Medical charts and consultation records of all patients aged between 18 and 30, diagnosed with TRS, evaluated during 1-year in our department, were selected for analysis. The most significant risk factors for TRS found in univariate model were younger age at schizophrenia onset, male gender, living in rural areas, co-morbid drug dependence, lower therapeutic adherence, and premorbid personality disorder. Marginally significant were higher Positive and Negative Syndrome Scale (PANSS) scores at previous admissions, higher scores on PANSS negative symptoms sub-scale, and lower educational background. In the multivariate model, TRS was still significantly predicted.
Therapeutic management of schizophrenia and substance use disorders dual diagnosis – clinical vignettes
Authors: Vasiliu Octavian
Published: April 2022

Patients with schizophrenia are frequently diagnosed with addictive comorbidities, and data in the literature support a 10 to 70% prevalence of this dual diagnosis. Nonetheless, substance use disorders can be missed during the initial interview with a psychotic patient, if the clinician is focused only on the more obvious manifestations. Therefore, using psychometric scales and structured interviews in patients with schizophrenia is strongly encouraged because the case manager will base his/her therapeutic decisions on quantifiable data about these patients’ symptoms and functional status. Clinical management in dual diagnosis cases must address both conditions simultaneously, as the delay in the initiation of substance withdrawal treatment may hinder the recovery from a psychotic episode. An important issue is represented by the potential pharmacologic interactions between drugs administered for schizophrenia and those targeting substance withdrawal and substance dependence. Other important aspects refer to (1) the therapeutic adherence, which can influence the prognosis of both conditions, (2) the negative impact of residual psychotic symptoms and substance-related disorders over the patient quality of life and daily functioning, (3) the necessity to integrate variables like the patient’s specific needs, lifestyle, and psychological resources in the therapeutic decision. These clinical vignettes are focused on clinical, biological, psychometric, and pharmacological dimensions, supporting the formulation of treatment recommendations based on monitoring both psychiatric and biological profiles.
The strategic importance of vaccination for national defense and security
Authors:   1 Military Medical Research  Center, “Titu Maiorescu”  University, Ordeanu Viorel
Published: April 2022

Public health is the most important power factor of a nation, from which all other factors derive. Infectious diseases are the most important cause of morbidity and mortality in humans. Vaccination is the most important method of prophylaxis of infectious-contagious diseases. Mass implementation of vaccination prevents the occurrence of epidemics, epizootic diseases or homonymous pandemics, and at the individual level ensures the specific active immunity (total or partial). So the vaccination of the population and/or the risk groups is of strategic importance to the nation, both in peacetime and in time of war. The national antidote production along with anti-infectives (vaccines, therapeutic serums, immunomodulators, antimicrobial chemotherapies and pesticides) has a strategic importance for the health of the population and of the troops. Biological crisis situations are cases of force majeure, where the Medicines Law provides the exception for manufacturing and use, even without a ""marketing authorization"". The military scientific research could make micro-production of specific drugs (antidotes, antiinfectives) for the needs of the army and the risk groups of the population. An example would be the preparation of vaccines for the prophylaxis of diseases caused by BWA and bioterrorism as niche products. Nothing hinders the preparation of any medication, such as magistral and galenical preparations, or pilot batch, provided there is adequate space, equipment, staff and procedures according to the law or the exception.