bluuebunny/crossref_metadata_2025 huggingface dataset:
Submagmatic to Solid-State Deformation Microstructures Recorded in Cooling Granitoids during Exhumation of Late-Variscan Crust in North-Eastern Sicily
Authors: Fazio Eugenio , Fiannacca Patrizia , Russo Damiano , Cirrincione Rosolino
Published: August 2020

Late-Variscan granitoid rocks of trondhjemitic and granitic composition, intruded in migmatitic paragneisses in the north-eastern Peloritani Mountains (southern Italy) at ~310 Ma and ~300 Ma, respectively, exhibit a range of deformation microstructures developed under a shear regime at decreasing temperatures. Non-coaxial deformation is documented by sigmoidal feldspar porphyroclasts, mica fish, and asymmetric boudins affecting tiny andalusite crystals. Late-Variscan shearing during granitoid cooling is constrained by largely represented chessboard patterns in quartz and, especially, submagmatic fractures in plagioclase, indicating deformation at high-temperature conditions (T > 650 °C), in the presence of melt. Submagmatic deformation was extensively superseded by deformation at lower temperatures. Examples of solid state-high temperature deformation-related microstructures (T > 450 °C) include feldspar bulging, quartz grain boundary migration, and subgrain rotation recrystallization. Finally, low temperature subsolidus microstructures (T < 450 °C) consist of quartz bulging, mica kinks, and feldspar twinning and bending. A complete sequence of deformation, operating from submagmatic to low-temperature subsolidus conditions is recorded in both the older and younger granitoids, suggesting a duration of ~20 Ma for shear zone activity during post-collisional exhumation of the Variscan middle crust in southernmost Italy.
A Single Case Report of Granular Cell Tumor of the Tongue Successfully Treated through 445 nm Diode Laser
Authors: Viani Maria Vittoria , Corcione Luigi , Di Blasio Chiara , Bologna-Molina Ronell , Vescovi Paolo , Meleti Marco
Published: August 2020

Oral granular cell tumor (GCT) is a relatively rare, benign lesion that can easily be misdiagnosed. Particularly, the presence of pseudoepitheliomatous hyperplasia might, in some cases, lead to the hypothesis of squamous cell carcinoma. Surgical excision is the treatment of choice. Recurrence has been reported in up to 15% of cases treated with conventional surgery. Here, we reported a case of GCT of the tongue in a young female patient, which was successfully treated through 445 nm diode laser excision. Laser surgery might reduce bleeding and postoperative pain and may be associated with more rapid healing. Particularly, the vaporization effect on remnant tissues could eliminate GCT cells on the surgical bed, thus hypothetically leading to a lower rate of recurrence. In the present case, complete healing occurred in 1 week, and no recurrence was observed after 6 months. Laser surgery also allows the possibility to obtain second intention healing. Possible laser-induced histopathological artifacts should be carefully considered.
Analysis of Risk Factors on Readmission Cases of COVID-19 in the Republic of Korea: Using Nationwide Health Claims Data
Authors: Jeon Woo-Hwi , Seon Jeong Yeon , Park So-Youn , Oh In-Hwan
Published: August 2020

In South Korea, 4.5% patients of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were readmitted to hospitals after discharge. However, there is insufficient research on risk factors for readmission and management of patients after discharge is poor. In this study, 7590 confirmed coronavirus disease (COVID-19) patients were defined as a target for analysis using nationwide medical claims data. The demographic characteristics, underlying diseases, and the use of medical resources were used to examine the association with readmission through the chi-square test and then logistic regression analysis was performed to analyze factors affecting readmission. Of the 7590 subjects analyzed, 328 patients were readmitted. The readmission rates of men, older age and patients with medical benefits showed a high risk of readmission. The Charlson Comorbidity Index score was also related to COVID-19 readmission. Concerning requiring medical attention, there was a higher risk of readmission for the patients with chest radiographs, computed tomography scans taken and lopinavir/ritonavir at the time of their first admission. Considering the risk factors presented in this study, classifying patients with a high risk of readmission and managing patients before and after discharge based on priority can make patient management and medical resource utilization more efficient. This study also indicates the importance of lifestyle management after discharge.
Prediction Equations of the Multifrequency Standing and Supine Bioimpedance for Appendicular Skeletal Muscle Mass in Korean Older People
Authors: Jeon Kwon Chan , Kim So-Young , Jiang Fang Lin , Chung Sochung , Ambegaonkar Jatin P. , Park Jae-Hyeon , Kim Young-Joo , Kim Chul-Hyun
Published: August 2020

Bioimpedance analysis (BIA) has been demanded for the assessment of appendicular skeletal muscle mass (ASM) in clinical and epidemiological settings. This study aimed to validate BIA equations for predicting ASM in the standing and supine positions; externally to cross-validate the new and published and built-in BIA equations for group and individual predictive accuracy; and to assess the overall agreement between the measured and predicted ASM index as sarcopenia diagnosis. In total, 199 healthy older adults completed the measurements of multifrequency BIA (InBody770 and InBodyS10) and dual-energy X-ray absorptiometry (DXA). Multiple regression analysis was used to validate the new multifrequency bioelectrical impedance analysis (MF-BIA) prediction equations. Each MF-BIA equation in the standing and supine position developed in the entire group included height2/resistance, sex, and reactance as predictors (R2 = 92.7% and 92.8%, SEE = 1.02 kg and 1.01 kg ASM for the standing and supine MF-BIA). The new MF-BIA equations had a specificity positive predictive value and negative predictive value of 85% or more except for a sensitivity of about 60.0%. The new standing and supine MF-BIA prediction equation are useful for epidemiological and field settings as well as a clinical diagnosis of sarcopenia. Future research is needed to improve the sensitivity of diagnosis of sarcopenia using MF-BIA.
Fake News and Covid-19 in Italy: Results of a Quantitative Observational Study
Authors: Moscadelli Andrea , Albora Giuseppe , Biamonte Massimiliano Alberto , Giorgetti Duccio , Innocenzio Michele , Paoli Sonia , Lorini Chiara , Bonanni Paolo , Bonaccorsi Guglielmo
Published: August 2020

During the Covid-19 pandemic, risk communication has often been ineffective, and from this perspective “fake news” has found fertile ground, both as a cause and a consequence of it. The aim of this study is to measure how much “fake news” and corresponding verified news have circulated in Italy in the period between 31 December 2019 and 30 April 2020, and to estimate the quality of informal and formal communication. We used the BuzzSumo application to gather the most shared links on the Internet related to the pandemic in Italy, using keywords chosen according to the most frequent “fake news” during that period. For each research we noted the numbers of “fake news” articles and science-based news articles, as well as the number of engagements. We reviewed 2102 articles. Links that contained fake news were shared 2,352,585 times, accounting for 23.1% of the total shares of all the articles reviewed. Our study throws light on the “fake news” phenomenon in the SARS-CoV-2 pandemic. A quantitative assessment is fundamental in order to understand the impact of false information and to define political and technical interventions in health communication. Starting from this evaluation, health literacy should be improved by means of specific interventions in order to improve informal and formal communication.
The Social Determinants of Health: Time to Re-Think?
Authors: Frank John , Abel Thomas , Campostrini Stefano , Cook Sarah , Lin Vivian K. , McQueen David V.
Published: August 2020

Twelve years have now passed since the influential WHO Report on the Social Determinants of Health (SDoH) in 2008. A group of senior international public health scholars and decision-makers met in Italy in mid-2019 to review the legacy of the SDoH conceptual framework and its adequacy for the many challenges facing our field as we enter the 2020s. Four major categories of challenges were identified: emerging “exogenous” challenges to global health equity, challenges related to weak policy and practice implementation, more fundamental challenges related to SDoH theory and research, and broader issues around modern research in general. Each of these categories is discussed, and potential solutions offered. We conclude that although the SDoH framework is still a worthy core platform for public health research, policy, and practice, the time is ripe for significant evolution.
Construction, Factor Structure, and Internal Consistency Reliability of the Hospital Physical Therapy Perceived Satisfaction Questionnaire (H-PTPS)
Authors: Albornoz-Cabello Manuel , Pérez-Mármol José Manuel , Cardero-Durán Mª de los Ángeles , Barrios-Quinta Cristo Jesús , Espejo-Antúnez Luis
Published: August 2020

Patient satisfaction is a crucial aspect in the evaluation of the quality of health care provided by health services and units, especially in patients that require physical rehabilitation. This study aims to design and analyze the factor structure and internal consistency reliability of the Hospital Physical Therapy Perceived Satisfaction Questionnaire (H-PTPS) measuring the level of physical therapy patient satisfaction in hospital rehabilitation services. This study has a multicenter cross-sectional survey design. This study used the structural validity and internal consistency domains from COSMIN (consensus-based standards for the selection of health status measurement instruments) guideline. The H-PTPS questionnaire consists of 20 closed questions. A sample of 384 adult patients from physical therapy units from three Spanish public hospitals completed this questionnaire. A factor structure and internal consistency reliability analysis were performed. The factor analysis including the 20 items of the H-PTPS showed an adequacy index of 0.922 according to the Kaiser–Meyer–Olkin measure and the Barlett test allowed us to reject the null hypothesis (p < 0.001). In the rotated component matrix, four specific factors were obtained, explaining 66.75% of the accumulated variance. All factors present satisfactory internal reliability, achieving Cronbach’s alpha indices and Omega coefficients higher than 0.74. The H-PTPS questionnaire has shown a four-factor solution with satisfactory reliability evaluating the satisfaction of Spanish patients treated in physical therapy units in the hospital rehabilitation services.
Changes in Lipid Profile, Body Weight Variables and Cardiovascular Risk in Obese Patients Undergoing One-Anastomosis Gastric Bypass
Authors: Jiménez Jose-Maria , Carbajo Miguel-Angel , López María , Cao María-José , Rúiz-Tovar Jaime , García Sara , Castro María-José
Published: August 2020

Morbid obesity has a direct impact on the development of cardiovascular disease. One-anastomosis gastric bypass (OAGB) is an effective surgical technique for the control of body weight and the reduction of cardiovascular risk. This work examines the change in weight loss, lipid profile and cardiovascular risk in 100 patients (71 women, 29 men), mean age 42.61 ± 11.33 years at 3, 6, 9, 12, 18 and 24 months after OAGB. At 24 months post-surgery, mean body weight was significantly reduced compared to pre-operative values (116.75 ± 22.19 kg vs. 69.66 ± 13.07 kg), as were mean total cholesterol (201.86 ± 44.60 mg/dL vs. 172.99 ± 32.26 mg/dL), LDL (Low-Density Lipoprotein) cholesterol (126.90 ± 39.81 mg/dL vs. 96.28 ± 26.99 mg/dL), triglycerides (138.05 ± 78.45 mg/dL vs. 76.04 ± 30.34 mg/dL) and cardiovascular risk (total cholesterol/HDL (High-Density Lipoprotein) cholesterol: 4.32 ± 1.24 vs. 2.93 ± 0.71), while the mean HDL cholesterol concentration was significantly higher (49.09 ± 14.16 mg/dL vs. 61.98 ± 14.86 mg/dL) (all p < 0.001). In conclusion, OAGB surgery led to significant reductions in body weight, a significant improvement in the lipid profile, and a reduction in cardiovascular risk.
Carbon-Based Nanomaterials as Promising Material for Wastewater Treatment Processes
Authors: Piaskowski Krzysztof , Zarzycki Paweł K.
Published: August 2020

In the latest literature search, the technology based on graphite oxide (GO) nanomaterials exhibits a great potential in many aspects of wastewater treatment involving adsorption, photocatalysis, disinfection and membrane process. In this study experimental data involving the carbon element in different forms such as active carbon (AC), graphite and graphene oxide (GO) applied as the active reagents in wastewater treatment are summarized and discussed. The first step was to characterize the aforementioned carbon materials and nanoparticles using various complementary techniques. These include optical microscopy, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), Raman spectrophotometry and zeta potential measurements. The second issue was to design the relatively simple experiment enabling us to observe the physicochemical and biological effects of carbon nanoparticles in the presence of sewage water and/or active sludge. Obtained experimental data have been inspected using univariate and multivariate (principal component analysis, PCA) approaches confirming the complex interaction of GO nanoparticles with microorganisms that are present in activated sludge. This experiment enabled the collection of an initial data set to design different large scale investigations focusing on active nanoparticles affecting wastewater purification. PCA calculations clearly revealed that GO strongly affects the wastewater technological processes investigated. It is hoped that the described results will allow the design of smart environmental protection systems in the future.
Dietary Practices and Adolescent Obesity in Secondary School Learners at Disadvantaged Schools in South Africa: Urban–Rural and Gender Differences
Authors: Okeyo Alice P. , Seekoe Eunice , de Villiers Anniza , Faber Mieke , Nel Johanna H. , Steyn Nelia P.
Published: August 2020

South Africa has a high prevalence of obesity in black female adolescents and a paucity of knowledge regarding contributing dietary practices. The aim of this study was to assess the dietary practices and weight status of male and female adolescents at secondary schools in the Eastern Cape province in urban and rural areas. Sixteen schools and grade 8–12 learners (N = 1360) were randomly selected from three health districts comprising poor disadvantaged communities. A short unquantified food frequency questionnaire was used to collect data on learners’ usual eating practices with regards to weekly meal pattern, breakfast consumption, foods taken to school, takeaways, and snacks eaten while watching television (TV). Body mass index measurements were determined for each learner. Prevalence of combined overweight and obesity differed significantly between genders, 9.9% in males versus 36.1% in females (p < 0.001). Significant gender differences were noted regarding eating practices. Females had a higher frequency of eating sugary snacks (p < 0.001) and a lower frequency of eating breakfast (p < 0.01) than males. Females ate significantly more fried fish (p < 0.05), pizza (p < 0.05) fat cakes (fried dough balls) (p < 0.05), hotdogs (p < 0.01), candy (p < 0.001), cake (p < 0.01), and crisps (p < 0.001). Compared to urban areas, the frequency of eating breakfast (p < 0.01) and sugary snacks (p < 0.05) was significantly higher in rural areas. Significantly more learners in urban areas consumed boerewors (beef sausage) rolls (p = 0.027), hamburgers (p = 0.004), and soft drinks (p = 0.019), while more learners in the rural areas consumed cordial (p = 0.001). In conclusion, a high prevalence of combined overweight and obesity was found in black female adolescents and a high prevalence of poor dietary practices was observed, with significant gender and urban–rural differences.
Transcriptional Profiling and Biological Pathway(s) Analysis of Type 2 Diabetes Mellitus in a Pakistani Population
Authors: Noreen Zarish , Loffredo Christopher A. , Bhatti Attya , Simhadri Jyothirmai J. , Nunlee-Bland Gail , Nnanabu Thomas , John Peter , Khan Jahangir S. , Ghosh Somiranjan
Published: August 2020

The epidemic of type 2 diabetes mellitus (T2DM) is an important global health concern. Our earlier epidemiological investigation in Pakistan prompted us to conduct a molecular investigation to decipher the differential genetic pathways of this health condition in relation to non-diabetic controls. Our microarray studies of global gene expression were conducted on the Affymetrix platform using Human Genome U133 Plus 2.0 Array along with Ingenuity Pathway Analysis (IPA) to associate the affected genes with their canonical pathways. High-throughput qRT-PCR TaqMan Low Density Array (TLDA) was performed to validate the selected differentially expressed genes of our interest, viz., ARNT, LEPR, MYC, RRAD, CYP2D6, TP53, APOC1, APOC2, CYP1B1, SLC2A13, and SLC33A1 using a small population validation sample (n = 15 cases and their corresponding matched controls). Overall, our small pilot study revealed a discrete gene expression profile in cases compared to controls. The disease pathways included: Insulin Receptor Signaling, Type II Diabetes Mellitus Signaling, Apoptosis Signaling, Aryl Hydrocarbon Receptor Signaling, p53 Signaling, Mitochondrial Dysfunction, Chronic Myeloid Leukemia Signaling, Parkinson’s Signaling, Molecular Mechanism of Cancer, and Cell Cycle G1/S Checkpoint Regulation, GABA Receptor Signaling, Neuroinflammation Signaling Pathway, Dopamine Receptor Signaling, Sirtuin Signaling Pathway, Oxidative Phosphorylation, LXR/RXR Activation, and Mitochondrial Dysfunction, strongly consistent with the evidence from epidemiological studies. These gene fingerprints could lead to the development of biomarkers for the identification of subgroups at high risk for future disease well ahead of time, before the actual disease becomes visible.
Intergenerational Transmissions of Mother–Adolescent Smartphone Dependency: The Mediating Role of Negative Parenting and the Moderating Role of Gender
Authors: Kim Chanhee , Kang Kyung Im , Lee Nayoon
Published: August 2020

Given the prevalence and undesirable consequences of smartphone dependency among adolescents, it is necessary to explore the influencing factors of adolescent smartphone dependency. The aim of this study was to examine the intergenerational transmission of mother–adolescent smartphone dependency and the mediating role of negative parenting, moderated by adolescent gender. Data for 2541 middle school students (mean aged = 13 years)–mother dyads were obtained from the first wave of the Korean Children and Youth Panel Survey 2018 (KCYPS 2018). The moderated mediation model using Hayes PROCESS macro (Model 14) was employed to test the study hypothesis. The moderated mediation model revealed that maternal smartphone dependency was associated with adolescent smartphone dependency. Perceived negative parenting mediated this link and adolescent gender moderated the relationship between negative parenting and adolescent smartphone dependency, especially for adolescent girls. Our findings showed that both maternal smartphone dependency and negative parenting were determinants of adolescent smartphone dependency, suggesting that both factors were important for understanding these issues. Moreover, the mediating role of negative parenting (between maternal and adolescent smartphone dependency) implies that parental education programs designed to improve negative parenting may reduce adolescent smartphone dependency, especially for adolescent girls.
Validity and Reliability of the Korean Version of the Pregnancy Physical Activity Questionnaire
Authors: Han Jeong-Won , Kang Ji-Soon , Lee Hanna
Published: August 2020

The purpose of this study was to translate the Pregnancy Physical Activity Questionnaire, a semi-quantitative tool that asks participants about time spent on 32 activities, into Korean and verify its validity and reliability. In total, 363 pregnant women under prenatal care at an obstetrics and gynecology hospital and a postpartum care facility in Gyeonggi-do completed the Korean version of the Pregnancy Physical Activity Questionnaire. The questionnaire’s content validity, construct validity, concurrent validity, and reliability were verified. After verifying the validity of the contents of the Pregnancy Physical Activity Questionnaire, all the questions were included in the Korean version. For construct validity, we divided the participants into primipara and multipara groups based on their delivery history. On comparison of the two groups’ physical activity based on the responses to the Pregnancy Physical Activity Questionnaire, there was a statistically significant difference in the total activity (t = −4.56, p < 0.001) and the total activity (light activity or more) (t = −5.80, p < 0.001). The correlation between the Pregnancy Physical Activity Questionnaire and the Global Physical Activity Questionnaire was tested to establish concurrent validity, and a significant correlation was found between all items except for vigorous physical activity. The Guttmann reliability coefficient by the odd-even method was 84. The Korean version of the Pregnancy Physical Activity Questionnaire is a suitable tool to measure the physical activity of pregnant women and can be used in clinical practice.
Continuous Work Support Checklist for Female Healthcare Workers: Scale Development and Validation
Authors: Ono Mariko , Nagasawa Toru , Ohkubo Takayoshi , Tsuchiya Akiko , Hiraike Haruko , Okinaga Hiroko , Nomura Kyoko
Published: August 2020

Healthcare jobs are very popular among women, however in Japan, women readily quit working because of gender-role responsibilities. This study aimed to develop a workplace support checklist for women to continue to work. In 2017, we investigated 780 (female 74.8%) faculty members and healthcare professionals of one medical university in Japan. We asked them to score the extent to which they considered 35 items identified by a task team, to be related to continuous work support for female workers in healthcare. We carried out an exploratory factor analysis and extracted four domains with 16 items in all: “Support for child rearing and home care” (five items), “Information dissemination” (five items), “Active promotion of women workers to higher positions” (three items), and “Consulting and counseling service” (three items), with Cronbach’s alpha values ranging from 0.88 to 0.92. We found that the first three factors were generally associated with reasonably relevant characteristics of being female, in their 30s, married, and members of faculty. We also found that women with “Intention to leave” the workplace underscored the importance of “Support for child rearing and home care” and “Consulting and counseling service”. These results suggest that the checklist is reliable and valid.
Industrial Energy-Related CO2 Emissions and Their Driving Factors in the Yangtze River Economic Zone (China): An Extended LMDI Analysis from 2008 to 2016
Authors: Ye Linlin , Wu Xiaodong , Huang Dandan
Published: August 2020

As the world’s largest developing country in the world, China consumes a large amount of fossil fuels and this leads to a significant increase in industrial energy-related CO2 emissions (IECEs). The Yangtze River Economic Zone (YREZ), accounting for 21.4% of the total area of China, generates more than 40% of the total national gross domestic product and is an important component of the IECEs from China. However, little is known about the changes in the IECEs and their influencing factors in this area during the past decade. In this study, IECEs were calculated and their influencing factors were delineated based on an extended logarithmic mean Divisia index (LMDI) model by introducing technological factors in the YREZ during 2008–2016. The following conclusions could be drawn from the results. (1) Jiangsu and Hubei were the leading and the second largest IECEs emitters, respectively. The contribution of the cumulative increment of IECEs was the strongest in Jiangsu, followed by Anhui, Jiangxi and Hunan. (2) On the whole, both the energy intensity and R&D efficiency play a dominant role in suppressing IECEs; the economic output and investment intensity exert the most prominent effect on promoting IECEs, while there were great differences among the major driving factors in sub-regions. Energy structure, industrial structure and R&D intensity play less important roles in the IECEs, especially in the central and western regions. (3) The year of 2012 was an important turning point when nearly half of these provinces showed a change in the increment of IECEs from positive to negative values, which was jointly caused by weakening economic activity and reinforced inhibitory of energy intensity and R&D intensity.
Work Shift and Circadian Rhythm as Risk Factors for Poor Sleep Quality in Public Workers from Murcia (Spain)
Authors: Rodríguez-González-Moro María Teresa , Rodríguez-González-Moro José Miguel , Rivera-Caravaca José Miguel , Vera-Catalán Tomás , Simonelli-Muñoz Agustín Javier , Gallego-Gómez Juana Inés
Published: August 2020

The purpose of this study was to determine the prevalence of sleep quality and to investigate variables predicting the risk of poor sleep quality in public workers from Murcia (Spain). A cross-sectional and prospective study was conducted from October 2013 to February 2016 in 476 public workers. The Pittsburgh Sleep Quality Index was used to measure the quality of sleep, and the reduced scale of the Horne and Österberg Morningness–Eveningness Questionnaire was applied to analyze the circadian typology. The predictive variables of self-reported poor sleep quality were identified by multivariate logistic regression. No significant differences were found according to sex in the overall sleep quality scores (5 ± 2.9 versus 5.1 ± 3, p = 0.650), but there were in the duration of sleep. Three percent of females slept <5 hours compared to 2% of men (p = 0.034). Fixed morning shifts (OR = 1.9, 95% CI 1.3–3.1; p = 0.007) and evening chronotypes (OR = 1.6, 95% CI 1.0–2.3; p = 0.017) were independent predictors of suffering from poor sleep quality. In conclusion, the frequency of self-reported poor sleep quality among public workers from Murcia was 37.4%. Being a public worker with a fixed morning shift and having an evening chronotype demonstrated to be associated with the quality of sleep.
The Protective Effect of Xanthohumol on the Content of Selected Elements in the Bone Tissue for Exposed Japanese Quails to TCDD
Authors: Całkosińska Aleksandra , Dominiak Marzena , Sobolewska Sylwia , Leśków Anna , Tarnowska Małgorzata , Całkosiński Aleksander , Dobrzyński Maciej
Published: August 2020

Dioxins (including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are highly toxic and persistent chemicals widely distributed in the environment in trace amounts, and are side products of industrial and chemical processes. Exposure to dioxins leads to multiorgan morphological and functional abnormalities, including within the bone tissue, disrupting its microarchitecture and mechanical properties. Xanthohumol (XN) is a chemical compound classified as a prenylated flavonoid, distinguished by multidirectional biological action. The aim of the study is to assess whether xanthohumol, as a substance with strong antioxidant and anti-inflammatory properties, has the ability to eliminate the negative effects of TCDD on bone tissue. The experiment was conducted on adult Japanese quails. Two different doses of TCDD and xanthohumol were administered to birds. After euthanasia of animals, the research material in the form of cranial vault and hind limb bone was collected, and their mineral compositions of calcium, phosphorus, magnesium, zinc, and iron concentrations were determined using atomic emission spectrometry in an acetylene-air flame method. Our results indicate that the administration of TCDD at a low dose causes more dynamic changes in the concentration of elements in bone, in comparison to a higher dose of dioxin. Results show also that higher doses of the XN cause the linear increase in the concentration of phosphorus and iron in the bone of the hind limb, and calcium in the bones of the cranial vault. In conclusion, our experiment shows that the use of TCDD and XN in Japanese quails together in various doses influences the content of phosphorus, magnesium, zinc, and iron in the research material.
Contribution of Motivational Climates and Social Competence in Physical Education on Overall Physical Activity: A Self-Determination Theory Approach with a Creative Physical Education Twist
Authors: Kokkonen Juha , Gråstén Arto , Quay John , Kokkonen Marja
Published: August 2020

Using a cross-sectional study design, we tested a structural equation model of hypothesized relationships among a group of variables: motivational climate in physical education (PE), students’ social competence in PE, out of-school physical activity (PA) motivation, PA intention and their moderate-to-vigorous PA (MVPA). Based on the self-reports of 363 fourth to sixth grade elementary school students (172 girls, 191 boys), the model revealed that the task-involving motivational climate in PE was linked to higher MVPA via cooperation in PE, and also via extrinsic motivation and PA intention. Ego-involving motivational climate was related to higher extrinsic motivation and amotivation, further to higher PA intention and, finally, to higher MVPA. Task-involving motivational climate was positively linked to students’ social competence markers of cooperation and empathy, and negatively to disruptiveness. Ego-involving motivational climate was positively related to disruptiveness and impulsivity, the markers of low social competence. The study showed that the motivational climate and co-operational aspect of social competence both played significant roles in students’ PA motivation, PA intention and MVPA. A pedagogical model that brings the learning of social competence relevant skills to the fore is creative physical education (CPE). Analysis of CPE is provided which highlights teaching behaviors which contribute to the students’ MVPA through motivational climates, co-operation, PA motivation and PA intention.
Increasing Health Literacy May Reduce Health Inequalities: Evidence from a National Population Survey in Ireland
Authors: Gibney Sarah , Bruton Lucy , Ryan Catherine , Doyle Gerardine , Rowlands Gillian
Published: August 2020

Background. Health literacy has been separately associated with socio-economic status and worse health status and outcomes. However, the magnitude of the associations between health literacy and health status and outcomes may not be evenly distributed across society. This study aims to estimate and compare the associations between health status, health behaviours, and healthcare utilisation within different levels of social status in the Irish population. Materials and methods. Data from Ireland collected as part of the 2011 European Health Literacy Survey were analysed. General health literacy was measured on a 0–50 scale, low to high. There were four binary outcomes: long-standing health conditions, smoking, hospital visits in the last 12 months, and self-rated health status. Logistic regression analysis was conducted to estimate the likelihood of each health outcome. Health literacy was treated as the main independent variable. Marginal effects were calculated using the delta method to demonstrate the change in likelihood of each outcome associated with a 5-point increase in health literacy score. The sample was grouped into tertiles based on self-reported social status, and models were replicated and compared for each tertile. Models were adjusted for known correlates of health literacy and health: age, gender, and education. Analysis was conducted using Stata V14. Results. Higher health literacy scores were associated with a lower probability of having a limiting illness within the low social status group only. Higher health literacy scores were associated with a lower probability of three or more hospital visits in the past 12 months in the low and middle social status groups. For people in the low and middle social status groups, higher health literacy levels were associated with a lower probability of being a current smoker. The associations between health literacy and self-rated health status were similar in each social status group. Conclusions: Improvement in population health literacy may reduce the prevalence of long-term chronic health conditions, reduce smoking levels, and result in fewer hospital visits. Whilst improved health literacy should improve behaviours and outcomes in all groups, it should have a more marked impact in lower social status groups, and hence contribute to reducing the observed social disparities in these health outcomes.
Effect of Triclosan Exposure on Developmental Competence in Parthenogenetic Porcine Embryo during Preimplantation
Authors: Kim Min Ju , Park Hyo-Jin , Lee Sanghoon , Kang Hyo-Gu , Jeong Pil-Soo , Park Soo Hyun , Park Young-Ho , Lee Jong-Hee , Lim Kyung Seob , Lee Seung Hwan , Sim Bo-Woong , Kim Sun-Uk , Cho Seong-Keun , Koo Deog-Bon , Song Bong-Seok
Published: August 2020

Triclosan (TCS) is included in various healthcare products because of its antimicrobial activity; therefore, many humans are exposed to TCS daily. While detrimental effects of TCS exposure have been reported in various species and cell types, the effects of TCS exposure on early embryonic development are largely unknown. The aim of this study was to determine if TCS exerts toxic effects during early embryonic development using porcine parthenogenetic embryos in vitro. Porcine parthenogenetic embryos were cultured in in vitro culture medium with 50 or 100 µM TCS for 6 days. Developmental parameters including cleavage and blastocyst formation rates, developmental kinetics, and the number of blastomeres were assessed. To determine the toxic effects of TCS, apoptosis, oxidative stress, and mitochondrial dysfunction were assessed. TCS exposure resulted in a significant decrease in 2-cell rate and blastocyst formation rate, as well as number of blastomeres, but not in the cleavage rate. TCS also increased the number of apoptotic blastomeres and the production of reactive oxygen species. Finally, TCS treatment resulted in a diffuse distribution of mitochondria and decreased the mitochondrial membrane potential. Our results showed that TCS exposure impaired porcine early embryonic development by inducing DNA damage, oxidative stress, and mitochondrial dysfunction.
Visualizing Single-Cell RNA-seq Data with Semisupervised Principal Component Analysis
Authors: Liu Zhenqiu
Published: August 2020

Single-cell RNA-seq (scRNA-seq) is a powerful tool for analyzing heterogeneous and functionally diverse cell population. Visualizing scRNA-seq data can help us effectively extract meaningful biological information and identify novel cell subtypes. Currently, the most popular methods for scRNA-seq visualization are principal component analysis (PCA) and t-distributed stochastic neighbor embedding (t-SNE). While PCA is an unsupervised dimension reduction technique, t-SNE incorporates cluster information into pairwise probability, and then maximizes the Kullback–Leibler divergence. Uniform Manifold Approximation and Projection (UMAP) is another recently developed visualization method similar to t-SNE. However, one limitation with UMAP and t-SNE is that they can only capture the local structure of the data, the global structure of the data is not faithfully preserved. In this manuscript, we propose a semisupervised principal component analysis (ssPCA) approach for scRNA-seq visualization. The proposed approach incorporates cluster-labels into dimension reduction and discovers principal components that maximize both data variance and cluster dependence. ssPCA must have cluster-labels as its input. Therefore, it is most useful for visualizing clusters from a scRNA-seq clustering software. Our experiments with simulation and real scRNA-seq data demonstrate that ssPCA is able to preserve both local and global structures of the data, and uncover the transition and progressions in the data, if they exist. In addition, ssPCA is convex and has a global optimal solution. It is also robust and computationally efficient, making it viable for scRNA-seq cluster visualization.
Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications
Authors: Rigogliuso Salvatrice , Salamone Monica , Barbarino Enza , Barbarino Maria , Nicosia Aldo , Ghersi Giulio
Published: August 2020

Cartilage is an avascular tissue with limited ability of self-repair. The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing. For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin. This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process. Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors. The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization. Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program. This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
The LL-100 Cell Lines Panel: Tool for Molecular Leukemia–Lymphoma Research
Authors: Drexler Hans G. , Quentmeier Hilmar
Published: August 2020

Certified cell line models provide ideal experimental platforms to answer countless scientific questions. The LL-100 panel is a cohort of cell lines that are broadly representative of all leukemia–lymphoma entities (including multiple myeloma and related diseases), rigorously authenticated and validated, and comprehensively annotated. The process of the assembly of the LL-100 panel was based on evidence and experience. To expand the genetic characterization across all LL-100 cell lines, we performed whole-exome sequencing and RNA sequencing. Here, we describe the conception of the panel and showcase some exemplary applications with a focus on cancer genomics. Due diligence was paid to exclude cross-contaminated and non-representative cell lines. As the LL-100 cell lines are so well characterized and readily available, the panel will be a valuable resource for identifying cell lines with mutations in cancer genes, providing superior model systems. The data also add to the current knowledge of the molecular pathogenesis of leukemia–lymphoma. Additional efforts to expand the breadth of available high-quality cell lines are clearly warranted.
KCND3-Related Neurological Disorders: From Old to Emerging Clinical Phenotypes
Authors: Pollini Luca , Galosi Serena , Tolve Manuela , Caputi Caterina , Carducci Carla , Angeloni Antonio , Leuzzi Vincenzo
Published: August 2020

KCND3 encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 defect causes both cardiological and neurological syndromes. From a neurological perspective, Kv4.3 defect has been associated to SCA type 19/22, a complex neurological disorder encompassing a wide spectrum of clinical features beside ataxia. To better define the phenotypic spectrum and course of KCND3-related neurological disorder, we review the clinical presentation and evolution in 68 reported cases. We delineated two main clinical phenotypes according to the age of onset. Neurodevelopmental disorder with epilepsy and/or movement disorders with ataxia later in the disease course characterized the early onset forms, while a prominent ataxic syndrome with possible cognitive decline, movement disorders, and peripheral neuropathy were observed in the late onset forms. Furthermore, we described a 37-year-old patient with a de novo KCND3 variant [c.901T>C (p.Ser301Pro)], previously reported in dbSNP as rs79821338, and a clinical phenotype paradigmatic of the early onset forms with neurodevelopmental disorder, epilepsy, parkinsonism-dystonia, and ataxia in adulthood, further expanding the clinical spectrum of this condition.
The Effect of Different Immunization Cycles of a Recombinant Mucin1-Maltose-Binding Protein Vaccine on T Cell Responses to B16-MUC1 Melanoma in Mice
Authors: Zhou Hongyue , Zhang Zenan , Liu Guomu , Jiang Mengyu , Wang Jingjing , Liu Yu , Tai Guixiang
Published: August 2020

We explored the effect of a recombinant mucin1-maltose-binding protein vaccine, including immunization cycles of recombinant mucin1-maltose-binding protein (MUC1-MBP) and CpG 2006 on T cell responses to human MUC1-overexpressing mouse melanoma B16 cells (B16-MUC1) melanoma in mice. We found that the vaccine had a significant antitumor effect, with the most obvious tumor-suppressive effect being observed in mice immunized five times. After more than five immunizations, the tumor inhibition rate decreased from 81.67% (five immunizations) to 43.67% (eight immunizations). To study the possible mechanism, Mucin-1(MUC1)-specific antibodies, IFN-γ secretion by lymphocytes, and cytotoxic T lymphocyte (CTL) cytotoxicity were measured by enzyme-linked immunosorbent assay (ELISA) and a real-time cell analyzer (RTCA). T cell subsets and immunosuppressive cells in the mouse spleen and tumor microenvironment were analyzed by FACS. These results showed that five immunizations activated MUC1-specific Th1 and CTL and reduced the ratio of myeloid-derived suppressor cells (MDSCs) and Th17 in mice more significantly than eight immunizations, indicating that excessive frequency of the immune cycle leads to the increased numbers of immunosuppressive cells and decreased numbers of immunostimulatory cells, thereby inhibiting antitumor immune activity. This data provide an experimental foundation for the clinical application of a recombinant MUC1-MBP vaccine.
EphrinA1-Fc Attenuates Ventricular Remodeling and Dysfunction in Chronically Nonreperfused WT but not EphA2-R-M mice
Authors: Whitehurst K’Shylah S. , Chan Victoria A. , Estes Heather K. , Valsaraj Smrithi , Kent Susan , Sharma Uma M. , Chase R. Christopher , Bhuiyan Maliha , Virag Jitka A. I.
Published: August 2020

Background: EphrinA1-Fc abolishes acute I/R injury and attenuates nonreperfused cardiac injury 4 days after permanent occlusion in mice. The goal of this study was to assess the capacity of a single intramyocardial administration of ephrinA1-Fc at the time of coronary artery ligation, to determine the degree to which early salvage effects translate to reduced adverse remodeling after 4 weeks of nonreperfused myocardial infarction (MI) in wild-type B6 and EphA2-R-M (EphA2 receptor null) mice. Methods: At 4 weeks post-MI, echocardiography, histologic and immunohistochemical analyses of B6 mouse hearts were performed. Primary mouse cardiac fibroblasts (FBs) isolated from B6 mice cultured in the presence of low and high dose ephrinA1-Fc, both with and without pro-fibrotic TGF-β stimulation and Western blots, were probed for relative expression of remodeling proteins MMP-2, MMP-9 and TIMP-1, in addition to DDR2 and (p)SMAD2/3/totalSMAD2/3. Results: EphrinA1-Fc preserved a significant degree of contractile function, decreased adverse left ventricular remodeling, attenuated excessive compensatory hypertrophy, and decreased interstitial fibrosis in wild-type (WT) B6 mouse hearts. In contrast, most of these parameters were poorer in ephrinA1-Fc-treated EphA2-R-M mice. Of note, fibrosis was proportionately decreased, implying that other EphA receptor(s) are more important in regulating the pro-fibrotic response. Primary FBs showed disparate alteration of MMP-2, MMP-9 and TIMP-1, as well as DDR2 and p-SMAD2/3/totalSMAD2/3, which indicates that matrix remodeling and cardiac fibrosis in the injured heart are influenced by ephrinA1-Fc. Conclusion: This study demonstrates the capacity of a single administration of ephrinA1-Fc at the onset of injury to attenuate long-term nonreperfused post-MI ventricular remodeling that results in progressive heart failure, and the important role of EphA2 in mitigating the deleterious effects.
Enforced C-Src Activation Causes Compartmental Dysregulation of PI3K and PTEN Molecules in Lipid Rafts of Tongue Squamous Carcinoma Cells by Attenuating Rac1-Akt-GLUT-1-Mediated Sphingolipid Synthesis
Authors: Wu Chien-Wei , Wang Shyang-Guang , Lee Ching-Hsiao , Chan Wen-Ling , Lin Meng-Liang , Chen Shih-Shun
Published: August 2020

Pharmacologic intervention to affect the membrane lipid homeostasis of lipid rafts is a potent therapeutic strategy for cancer. Here we showed that gallic acid (GA) caused the complex formation of inactive Ras-related C3 botulinum toxin substrate 1 (Rac1)-phospho (p)-casein kinase 2 α (CK2α) (Tyr 255) in human tongue squamous carcinoma (TSC) cells, which disturbed the lipid raft membrane-targeting of phosphatidylinositol 3-kinase (PI3K)-Rac1-protein kinase B (Akt) signal molecules by inducing the association of p110α-free p85α with unphosphorylated phosphatase tensin homolog deleted on chromosome 10 (PTEN) in lipid rafts. The effects on induction of inactive Rac1-p-CK2α (Tyr 255) complex formation and attenuation of p-Akt (Ser 473), GTP-Rac1, glucose transporter-1 (GLUT-1) lipid raft membrane-targeting, and cell invasive activity by GA were counteracted either by CK2α short hairpin RNA or cellular-Src (c-Src) inhibitor PP1. PP1 treatment, GLUT-1 or constitutively active Rac1 ectopic-expression blocked GA-induced decreases in cellular glucose, sphingolipid and cholesterol of lipid raft membranes, p85α-p110α-GTP-Rac1 complexes, glucosylceramide synthase activity and increase in ceramide and p110α-free p85α-PTEN complex levels of lipid raft membranes, which reversed the inhibition on matrix metalloproteinase (MMP)-2/-9-mediated cell invasion induced by GA. Using transient ectopic expression of nuclear factor-kappa B (NF-κB) p65, MMP-2/-9 promoter-driven luciferase, and NF-κB-dependent luciferase reporter genes and NF-κB specific inhibitors or Rac1 specific inhibitor NSC23766, we confirmed that an attenuation of Rac1 activity by GA confers inhibition of NF-κB-mediated MMP-2/-9 expression and cell invasion. In conclusion, GA-induced c-Src activation is a key inductive event for the formation of inactive Rac1-p-CK2α (Tyr 255) complexes, which disturbed lipid raft compartment of PI3K and PTEN molecules by impairing Akt-regulated GLUT-1-mediated sphingolipid synthesis, and finally resulting in inhibition of TSC cell invasion.