bluuebunny/crossref_metadata_2025 huggingface dataset:
Are Growth Performance and Fecal Score in Weaning Pigs Affected by the Inclusion Level of Potato Protein Concentrate and the Enclosed Glycoalkaloids in Iso-Nitrogenous Diets?
Authors: Voergaard Annette Lykke , de Knegt Leonardo Victor , Madsen Johannes Gulmann , Dall Jacob
Published: October 2023

Glycoalkaloids (GA) are anti-nutritional factors in standard potato protein concentrate (PPC) fed to piglets. Increasing levels of standard PPC was expected to affect growth performance and fecal score negatively. Seven-hundred-and-twenty pigs (7–30 kg) were fed one of the following four diets within three feeding phases (days 0–13, 13–24, and 24–45): control (CTRL), PPC standard inclusion (PPC-S; 4%, 2%, and 0%), high PPC inclusion (PPC-H; 8%, 3.5%, and 2%), and extremely high PPC inclusion (PPC-EH; 12%, 5%, and 3.5%). During days 0–13, CTRL displayed no difference in growth performance compared with the three experimental groups (PPC-S, PPC-H, and PPC-EH). During days 13–24, PPC-H achieved greater (p < 0.001) average daily feed intake (ADFI) compared to CTRL. During days 24–45, no differences between groups were observed. For the overall experimental period (0–45 days), PPC-H displayed greater average daily gain (ADG) (p = 0.010) and ADFI (p = 0.024) compared to CTRL. The feed conversion ratio (FCR) remained unaffected between the groups for all experimental periods. Increasing levels of PPC and hence GA did not affect the probability of diarrhea. In conclusion, increased standard PPC and hence increased levels of GA in isonitrogenous diets did not negatively affect growth performance nor fecal score in piglets (7–30 kg).
Intravitreal Injection of ZYAN1 Restored Autophagy and Alleviated Oxidative Stress in Degenerating Retina via the HIF-1α/BNIP3 Pathway
Authors: Hao Xiao-Na , Zhao Na , Huang Jie-Min , Li Si-Yu , Wei Dong , Pu Ning , Peng Guang-Hua , Tao Ye
Published: October 2023

Mitochondrial autophagy plays a contributary role in the pathogenesis of retina degeneration (RD). ZYAN1 is a novel proline hydroxylase domain (PHD) inhibitor that can enhance the expression of hypoxia-inducible factor 1-alpha (HIF-1α). This study investigated whether ZYAN1 could alleviate progressive photoreceptor loss and oxidative damage in a pharmacologically induced RD model via the modulation of mitophagy. ZYAN1 was injected into the vitreous body of the RD model, and the retinal autophagy level was analyzed. The therapeutic effects of ZYAN1 were evaluated via a function examination, a morphological assay, in situ reactive oxygen species (ROS) detection, and an immunofluorescence assay. It was shown that the thickness of the outer nuclear layer (ONL) increased significantly, and visual function was efficiently preserved via ZYAN1 treatment. The mitochondria structure of photoreceptors was more complete in the ZYAN1-treated mice, and the number of autophagosomes also increased significantly. Membrane disc shedding and ROS overproduction were alleviated after ZYAN1 treatment, and the axonal cilia were more structurally intact. A Western blot analysis showed that the expression levels of the autophagy-related proteins LC3-B, Beclin-1, and ATG5 increased significantly after ZYAN1 treatment, while the expression of P62 was down-regulated. Moreover, the expression levels of HIF-1α and BNIP3 were up-regulated after ZYAN1 treatment. Therefore, an intravitreal injection of ZYAN1 can act as part of the pharmacologic strategy to modulate mitophagy and alleviate oxidative stress in RD. These findings enrich our knowledge of RD pathology and provide insights for the discovery of a therapeutic molecule.
Federated and Transfer Learning Applications
Authors: Drosatos George , Efraimidis Pavlos S. , Arampatzis Avi
Published: October 2023

The classic example of machine learning is based on isolated learning—a single model for each task using a single dataset [...]
Research on Detection and Restoration Methods of Basic Operation Data for Inter-Basin Water Diversion Projects
Authors: Lu Mengyao , Xu Guitao , Liu Xiaolian
Published: October 2023

Inter-basin water diversion is an essential means to alleviate the contradiction between the supply and demand of water resources, and accurate hydraulic modelling guarantees smooth operation. However, due to the increasing complexity of water diversion methods, structures, water conservancy facilities and equipment, it is difficult to obtain accurate and effective measured data to establish a model. Therefore, based on a data-driven method, this paper diagnoses and restores the important parameters of the water diversion projects, including the elevation of pipeline and water level data, which can be used to establish the adaptive hydraulic transition model of the water diversion projects. Firstly, the abnormal data of the elevation of pipeline were identified using expert data annotation and support vector classification (SVC), with the identification accuracy of abnormal data being as high as 91%. Then, the single and continuous abnormal data were restored using an interpolation method and multiple linear regression algorithm (MLR), and the restored data were found to be consistent with the judgment of expert knowledge. Secondly, K-medoids was used to classify the complex multi-dimensional water level data, combined with the 3-sigma method to identify the outliers in each class. The gradient boosting decision tree algorithm (GBDT) trained on normal data restored outliers in a predictive manner, and the mean absolute percentage error (MAPE) was 0.003%, 0.025% and 0.091% in each class, respectively, showing the best accuracy compared with other models.
Experimental Study of Radiated Emission Due to Secondary Common-Mode Current in Buck Converters
Authors: Takahashi Shotaro
Published: October 2023

High switching frequency and fast switching speed of wide bandgap power semiconductor device-based power converters may increase radiated emissions more than conventional silicon-power device-based power converters. Based on the background, this article experimentally investigates radiated emission sources in buck converters. The experimental system constructed in this article has two common-mode noise paths, the primary common-mode and the secondary common-mode. First, this article outlines that a secondary common-mode voltage source is caused by a primary common-mode current and an imbalance of power supply side impedances, and the secondary common-mode current path acts as a monopole antenna. The common-mode currents and the radiated emissions are measured in an anechoic chamber when the common-mode inductor for each mode is connected. The measurement results show that the primary common-mode current generates the secondary common-mode noise, and the secondary common-mode noise is the dominant source of radiated emission in the experimental system.
Automated Control of the Fresh State of Industrial Concrete Behaviour by Rheometer Test Adjustment
Authors: Rojo-López Gemma , González-Fonteboa Belén , Carro-López Diego , Martínez-Abella Fernando
Published: October 2023

This study aimed to develop a rheometer prototype and define a procedure for adjusting the automated control of the fresh state of concrete. Sixteen batches were produced, and their fresh behaviour was measured at different testing times by applying the Abrams cone and flow curve test (FCT) as the rheological protocol. During this test, the yield stress and plastic viscosity of the concretes were measured in relative units. The rheometer prototype was used to define a new protocol to select the most suitable rheometer impeller arrangement and optimal FCT configuration. This protocol considers the torque at the end of the breakdown period, torque reduction during the breakdown period, segregation, and negative values of the yield stress in relative units. This protocol also enabled an iterative adjustment procedure, facilitating the use of a rheometer for the automated control of the homogeneity and behaviour of fresh concrete, as well as real-time decision making.
Development of a Specific Lexicon to Describe Sensory and Textural Characteristics of Olive Paté
Authors: Lanza Barbara , Bacceli Martina , Simone Nicola
Published: October 2023

Olive paté is a traditional Italian food typical of the Mediterranean diet, derived from debittered, pitted, and crushed table olives. Currently, there is no sensory standard for the quality of the product. In this research, a specific lexicon and an innovative profile sheet for sensory analysis of olive paté have been developed and tested to fill the gap. The list of descriptors was set in terms of negative sensations, olfactory/gustatory sensations, and kinaesthetic attributes. The assessors of a professional panel were first trained with and then used the profile sheet to characterize 40 olive paté commercial samples from five different Olea europaea L. cultivars. From a sensorial point of view, Nocellara del Belice paté was perceived as the saltiest, Nocellara etnea as the bitterest, and Hojiblanca and Bella di Cerignola were the most balanced and characterized on the olfactory/gustatory level. In terms of texture, Hojiblanca paté is the most consistent and grainiest. To support and verify kinaesthetic descriptors, textural analysis by back extrusion was performed. Through the agglomerative hierarchical analysis, the various paté showed a clear clusterization into two clusters: Nocellara del Belice-Itrana and Hojblanca-Nocellara etnea-Bella di Cerignola. The results obtained showed the validity of the profile sheet to correctly assess the different products.
RETRACTED: De Aza et al. A Si-αTCP Scaffold for Biomedical Applications: An Experimental Study Using the Rabbit Tibia Model. Appl. Sci. 2017, 7, 706
Authors: De Aza Piedad N. , Rodríguez Miguel A. , Gehrke Sergio A. , Maté-Sánchez de Val José E. , Calvo-Guirado Jose L.
Published: October 2023

The journal retracts the article “A Si-αTCP Scaffold for Biomedical Applications: An Experimental Study Using the Rabbit Tibia Model” [...]
Experimental Study and Phenomenological Laws of Some Nonlinear Behaviours of the Wheel–Rail Contact Associated with the Deshunting Phenomenon
Authors: Kaza Guy-Léon , Houzé Frédéric , Loëte Florent , Testé Philippe
Published: October 2023

A widely used technique for locating the position of a train during its journey involves electrical detection: on a section of track, the train closes (“shunts”) a circuit dedicated to its location via its wheels and axles. The quality of the wheel–rail contact is therefore particularly important for signalling management and traffic control. In this paper, we show that the current flowing through the track circuit induces a permanent modification and a nonlinear, frequency-dependent behaviour of the electrical contact. We propose an analytical expression that describes the evolution of the measured voltage as a function of current and frequency for these nonlinear behaviours. This behavioural law was obtained using experimental measurements on a real train and could therefore be integrated into a global model describing the track system.
Evaluation of the Effects of Passive Lower-Limb Exoskeletons on Muscle Activities According to Working Heights
Authors: Shim Hyun-Ho , Choi Kyeong-Hee , Keum Hyunji , Son Seungjae , Kim Jeong Ho , Seo Min-Tae , Kim Seoung-Yeon , Park Donghyun , Kong Yong-Ku
Published: October 2023

Introduction: Research and development efforts regarding passive lower-limb exoskeletons are actively ongoing to reduce work-related musculoskeletal disorders (WMDSs) from improper and prolonged posture. The aims of this study were to evaluate the effects of passive lower-limb exoskeletons and working heights on muscle activity and subjective comfort ratings. Methods: In this study, 20 males performed a 10-min drilling task for three levels of working height (60, 85, and 110 cm), and three levels of intervention (WO: without exoskeleton, WCEX, and WCC) were used as independent variables. The EMG data of eight muscles and subjective discomfort ratings in each of the six body parts were analyzed in this study. Results: The results of this study confirm that the effect of wearing a lower-limb exoskeleton device may vary depending on the muscle type and working height. Overall, the positive effects of wearing were generally observed in the lower-limb muscles, whereas the muscle activity of the upper limbs showed an increasing trend when wearing the device at a height of 85 cm or more. Conclusions: Therefore, to obtain positive effects in both the upper- and lower-limb muscles, using the lower-limb exoskeletons at a working height of 85 cm or less is recommended.
Analytical Solutions of Water Inflow for Foundation Pit in Confined Water Stratum
Authors: Shen Jingjing , Jiang Yue , Wu Jie , Li Pengfei
Published: October 2023

This paper is aimed at the problem of inaccurate calculation of the water inflow of a foundation pit with a suspended water-proof curtain in a confined water stratum. According to the groundwater hydraulics principle, the theoretical model of the seepage field of the foundation pit is established, and the analytical formula of the water inflow for the circular, strip, and rectangular foundation pits is deduced. The seepage path influence coefficient α related to the water-proof curtain insertion ratio, the thickness of the confined aquifer, and the radius of the foundation pit is proposed and obtained by the positive and negative analysis of numerical simulation. The coefficient is used to correct the equivalent seepage length in the pit and directly affect the calculation result of water inflow. Finally, based on the calculation results of the analytical solution of water inflow, the relationship between the scale of the foundation pit and the water control effect is discussed. The research results show that the theoretical analysis results in this paper are in good agreement with the numerical results and the measured data in the field: when the size of the foundation pit is too large, it is not suitable to use the suspended water-proof curtain as the water control measure.
Multi-Objective-Based Intelligent Lubrication System Performance Evaluation Technology for Construction Machinery
Authors: Peng Han , Chen Yike , Shangguan Linjian , Cheng Ruixue , Li Yanchi , Yang Can
Published: October 2023

The infrastructure construction process cannot be separated from construction machinery; it will inevitably produce wear and tear in the work. The level of wear and tear is severe and could cause mechanical accidents. There are safety hazards involved with wear and tear; thus, the study of the lubrication systems of construction machinery is crucial. This paper addresses the problems with the intelligent lubrication systems of construction machinery and establishes a performance evaluation index system for the intelligent lubrication systems of construction machinery by analyzing and selecting appropriate evaluation indexes. Based on the built evaluation system, a performance evaluation model was established based on the hierarchical analysis (analytic hierarchy process, AHP)–entropy weight method and a topological object element model. The feasibility of the model was tested using the example of an off-road mining dump truck. This model analyzes the performance strengths and weaknesses of smart lubrication systems and suggests improvement measures and recommendations for weak links. It also provides a reference for analyzing the performance of smart lubrication systems for other mechanical devices.
Loop-Type Field Probe to Measure Human Body Exposure to 5G Millimeter-Wave Base Stations
Authors: Kwon DukSoo , Lee Young Seung , Hyoung Chang-Hee , Hwang Jung-Hwan , Choi Hyung-Do
Published: October 2023

This paper proposes a field probe to measure the power density (PD) at a millimeter-wave (mmWave) frequency band. The proposed probe is composed of a loop antenna in which one end is terminated with a load resistor. Such a structure enables the simultaneous measurement of electric (E)- and magnetic (H)-fields: the E-field is measured at a gap where the load resistor is placed, and the H-field is measured through the loop antenna. The simultaneous measurement makes it possible to measure PD for an incident wave even in the near field region where the E- and H-fields have different phases from each other. The proposed probe is fabricated and evaluated for its PD measurement performance. The measurement results show that the probe measures PD with an error less than 1.3 dB. Owing to the near field measurement, the proposed probe is useful in measuring the human body exposure to electromagnetic fields (EMFs) that are generated by 5G mmWave base stations.
Molecular Docking and ADME-TOX Profiling of Moringa oleifera Constituents against SARS-CoV-2
Authors: Souza Hellen Cris Araújo , Souza Maycon Douglas Araújo , Sousa Cássio Silva , Viana Edilanne Katrine Amparo , Alves Sabrina Kelly Silva , Marques Alex Oliveira , Ribeiro Arthur Serejo Neves , de Sousa do Vale Vanessa , Islam Muhammad Torequl , de Miranda João Antônio Leal , da Costa Mota Marcelo , Rocha Jefferson Almeida
Published: October 2023

The SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2019) etiological agent, which has a high contagiousness and is to blame for the outbreak of acute viral pneumonia, is the cause of the respiratory disease COVID-19. The use of natural products grew as an alternative treatment for various diseases due to the abundance of organic molecules with pharmacological properties. Many pharmaceutical studies have focused on investigating compounds with therapeutic potential. Therefore, this study aimed to identify potential antiviral compounds from a popular medicinal plant called Moringa oleifera Lam. against the spike, Mpro, ACE2, and RBD targets of SARS-CoV-2. For this, we use molecular docking to identify the molecules with the greatest affinity for the targets through the orientation of the ligand with the receptor in complex. For the best results, ADME-TOX predictions were performed to evaluate the pharmacokinetic properties of the compounds using the online tool pkCSM. The results demonstrate that among the 61 molecules of M. oleifera, 22 molecules showed promising inhibition results, where the compound ellagic acid showed significant molecular affinity (−9.3 kcal.mol−1) in interaction with the spike protein. These results highlight the relevance of investigating natural compounds from M. oleifera as potential antivirals against SARS-CoV-2; however, additional studies are needed to confirm the antiviral activity of the compounds.
Design in a Colonial Periphery: Guilds, Artisans, and Non-Artisans in 18th-Century Sonsonate, El Salvador
Authors: Castellón Osegueda José Ricardo
Published: October 2023

Historical studies on the subject of Central American design are scarce. This article attempts to fill the gap as well as to overcome the exclusive correlation of design with industrialization. It highlights the relationship in a space and time other than those studied customarily: in the present case, 18th-century Sonsonate, El Salvador. With this purpose, it analyzes crafts, based primarily on an unpublished census of 1787 housed in the Archivo Municipal de Sonsonate. Considering design as a practice inherent to human life, this paper argues that design was present in craft activity through the creation of products, becoming a key factor for survival in a colonial periphery and overcoming guild barriers. Research findings indicate the limitations of conceiving design in industrial societies as a construction of coloniality, positing also the need to advance a concept of design that addresses the cultural and historical realities of peripheral regions like Sonsonate.
Ventilation Strategies for Mitigating Indoor Air Pollutants in High-Rise Residential Buildings: A Case Study in Dubai
Authors: Jung Chuloh , Abdelaziz Mahmoud Naglaa Sami
Published: October 2023

This study investigates the effectiveness of different ventilation methods in reducing indoor air pollutants in newly constructed residential buildings, focusing on indoor air quality (IAQ) in Dubai. The paper highlights the growing concern for IAQ in response to residents’ increasing awareness of their well-being and environmental sustainability. The study examines the concentrations of formaldehyde (HCHO), volatile organic compounds (VOCs), and total volatile organic compounds (TVOC) in bedrooms and living rooms before and after implementing various ventilation methods during the construction phase. The findings indicate that mechanical exhaust ventilation, mainly through bathroom and kitchen exhaust fans, was highly effective in reducing HCHO levels. Combining kitchen and bathroom exhaust fans demonstrated the most significant reduction in HCHO concentrations. Similarly, reductions in VOCs, such as ethylbenzene, toluene, and xylene, were observed with different ventilation methods. Natural ventilation also proved effective in reducing pollutant concentrations. The results emphasize the importance of implementing appropriate ventilation strategies to improve IAQ in residential buildings. However, the study acknowledges the limitations of a single-location measurement and recommends further research to validate the findings across different building types and locations. Additionally, long-term studies are necessary to assess the sustained effects of ventilation methods on IAQ. The study highlights the significance of addressing IAQ concerns in residential buildings and suggests potential research directions to explore other ventilation strategies and their energy efficiency implications. Ultimately, this research contributes to developing healthier and sustainable living environments by promoting effective ventilation strategies to mitigate indoor air pollutants.
Multiscale Analysis of a Record-Breaking Predecessor Rain Event Ahead of Typhoon Danas (2019) in Jiangsu, China
Authors: Wang Kun , Xia Xin , Wang Xiaohua , Li Min , Gu Peishu , Peng Xiaoyan
Published: October 2023

On 17 July 2019, an unusually intense rainfall occurred in the central-eastern part of Jiangsu Province in China, resulting in a record-breaking daily precipitation of 286.4 mm at the Rugao station, not seen since 1961. A comprehensive analysis was conducted on various multiscale characteristics of the initial rain event, such as the large-scale surroundings, moisture transport, triggering and maintenance mechanisms, and microphysical characteristics. Multi-sources of data were utilized, such as reanalysis data, automatic weather stations, wind-profiling radar, laser-optical Particle Size Velocity instruments, soundings, S-band dual-polarization radar, and a Lagrangian model. The findings suggest that the intense precipitation in Rugao resulted from the convergence of the warm and moist airflow from Typhoon Danas and the cold air moving southward from the north, along with the ample moisture and energy provided by the circulation of Typhoon Danas. Convection, which showed good consistency with the intense precipitation process, was initiated by mesoscale temperature gradients and wind field convergence. This was associated with the intrusion of a near-surface cold pool and the maintenance of a ground convergence line in the Rugao area. This convection exemplified a standard system of warm clouds with high precipitation efficacy. It had a high concentration of raindrops, especially large ones, resulting in record-breaking precipitation in a short amount of time.
Assessing the Accuracy of 3D-VAR in Supercell Thunderstorm Forecasting: A Regional Background Error Covariance Study
Authors: Samos Ioannis , Louka Petroula , Flocas Helena
Published: October 2023

Data assimilation (DA) integrates observational data with numerical weather predictions to enhance weather forecast accuracy. This study evaluates three regional background error (BE) covariance statistics for numerical weather prediction (NWP) via a variational data assimilation (VAR) scheme. The best practices in DA are highlighted, as well as the impact of BE covariance calculation in DA procedures by employing the Weather Research and Forecasting (WRF) model. Forecasts initialized at different intervals were used to compute distinct regional background error statistics utilizing three control variable (CV) methodologies over a span of 20 days. These statistics are used by the three-dimensional VAR DA process of WRF DA software, producing analysis fields that lead to forecasts for a distinct convective supercell event during the summer of 2019 over northern Greece. This high-impact convective event underscores the importance of selecting appropriate BE over complex terrain areas. The results emphasize the significance of BE usage in DA, proposing the optimal DA approach for simulations of convective systems.
Application of Migrating Optimization Algorithms in Problems of Optimal Control of Discrete-Time Stochastic Dynamical Systems
Authors: Panteleev Andrei , Rakitianskii Vladislav
Published: October 2023

The problem of finding the optimal open-loop control for discrete-time stochastic dynamical systems is considered. It is assumed that the initial conditions and external influences are random. The average value of the Bolza functional defined on individual trajectories is minimized. It is proposed to solve the problem by means of classical and modified migrating optimization algorithms. The modification of the migrating algorithm consists of cloning the members of the initial population and choosing different strategies of migratory behavior for the main population and for populations formed by clones. At the final stage of the search for an extremum, an intensively clarifying migration cycle is implemented with the participation of three leaders of the populations participating in the search process. Problems of optimal control of bundles of trajectories of deterministic discrete dynamical systems, as well as individual trajectories, are considered as special cases. Seven model examples illustrating the performance of the proposed approach are solved.
Differentiating Cyclability and Kinetics of Na+ Ions in Surface-Functionalized and Nanostructured Graphite Using Electrochemical Impedance Spectroscopy
Authors: Dey Sonjoy , Singh Gurpreet
Published: October 2023

The revolution in lithium-ion battery (LIB) technology was partly due to the invention of graphite as a robust negative electrode material. However, equivalent negative electrode materials for complementary sodium ion battery (NIB) technologies are yet to be commercialized due to sluggish reaction kinetics, phase instability, and low energy density originating from the larger size of Na+-ion. Therefore, in search of the next-generation electrode materials for NIBs, we first analyze the failure of graphite during reversible Na+ ion storage. Building upon that, we suggest surface-functionalized and nanostructured forms of analogous carbon allotropes for enhancing Na+ ion storage. During long-term rigorous cycling conditions, Graphene Oxide (GO) and Graphene nanoplatelets (GNP) exhibit higher Na+ ion storage (157 mAh g−1 and 50 mAh g−1 after 60 cycles, respectively) compared to graphite (27 mAh g−1). Optimizing alternative NIBs requires a comprehensive analysis of cycling behavior and kinetic information. Therefore, in this investigation, we further examine ex-situ electrochemical impedance spectroscopy (EIS) at progressive cycles and correlate capacity degradation with impedance arising from the electrolyte, solid electrolyte interphase formation, and charge transfer.
Automated Segmentation and Measurements of Pulmonary Cysts in Lymphangioleiomyomatosis across Multiple CT Scanner Platforms over a Period of Two Decades
Authors: Lee Simone , Lebron Alfredo , Matthew Brianna , Moss Joel , Wen Han
Published: October 2023

(1) Background: Lymphangioleiomyomatosis is a genetic disease that affects mostly women of childbearing age. In the lungs, it manifests as the progressive formation of air-filled cysts and is associated with a decline in lung function. With a median survival of 29 years after the onset of symptoms, computed-tomographic monitoring of cystic changes in the lungs is a key part of the management of the disease. However, the current standard method to measure cyst burdens from CT is semi-automatic and requires manual adjustments from trained operators to obtain consistent results due to variabilities in CT technology and imaging conditions over the long course of the disease. This can be impractical for longitudinal studies involving large numbers of scans and is susceptible to subjective biases. (2) Methods: We developed an automated method of pulmonary cyst segmentation for chest CT images incorporating novel graphics processing algorithms. We assessed its performance against the gold-standard semi-automated method performed by experienced operators who were blinded to the results of the automated method. (3) Results: the automated method had the same consistency over time as the gold-standard method, but its cyst scores were more strongly correlated with concurrent pulmonary function results from the physiology laboratory than those of the gold-standard method. (4) Conclusions: The automated cyst segmentation is a competent replacement for the gold-standard semi-automated process. It is a solution for saving time and labor in clinical studies of lymphangioleiomyomatosis that may involve large numbers of chest CT scans from diverse scanner platforms and protocols.
Proton Microbeam Targeted Irradiation of the Gonad Primordium Region Induces Developmental Alterations Associated with Heat Shock Responses and Cuticle Defense in Caenorhabditis elegans
Authors: Beaudier Pierre , Devès Guillaume , Plawinski Laurent , Dupuy Denis , Barberet Philippe , Seznec Hervé
Published: October 2023

We describe a methodology to manipulate Caenorhabditis elegans (C. elegans) and irradiate the stem progenitor gonad region using three MeV protons at a specific developmental stage (L1). The consequences of the targeted irradiation were first investigated by considering the organogenesis of the vulva and gonad, two well-defined and characterized developmental systems in C. elegans. In addition, we adapted high-throughput analysis protocols, using cell-sorting assays (COPAS) and whole transcriptome analysis, to the limited number of worms (>300) imposed by the selective irradiation approach. Here, the presented status report validated protocols to (i) deliver a controlled dose in specific regions of the worms; (ii) immobilize synchronized worm populations (>300); (iii) specifically target dedicated cells; (iv) study the radiation-induced developmental alterations and gene induction involved in cellular stress (heat shock protein) and cuticle injury responses that were found.
An Evaluation of the Crop Preference and Phenotypic Characteristics of Ceracris kiangsu Tsai (Orthoptera: Arcypteridae) under Different Temperatures
Authors: Wang Meizhi , Li Hongmei , Bukero Abdul Aziz , Shu Jinping , Zhuo Fuyan , Liu Linyi , Zhang Aihuan
Published: October 2023

The yellow-spined bamboo locust (YSBL), Ceracris kiangsu Tsai, has historically had a significant impact on different bamboo varieties in East Asia and Southeast Asia. Since 2014, there have been many outbreaks of YSBL populations in Laos, and YSBLs subsequently invaded Southwest China in 2020 and 2023. However, there was limited information about the damage to staple crops. Life table parameters and fitness parameters were assessed using wheat, rice, waxy maize, and sweet maize under three different temperatures (25 °C, 30 °C, and 35 °C) in the laboratory. The results indicated that the YSBLs feeding on wheat seedlings displayed a significantly higher survival rate, a shorter developmental time, and a higher adult emergence rate compared to YSBLs feeding on the other host species at 30 °C. The developmental durations of 1st and 3rd instar YSBLs on wheat (1st: 8.21 ± 0.35 d; 3rd: 6.32 ± 0.34 d) and rice (1st: 7.19 ± 0.23 d; 3rd: 9.00 ± 0.66 d) were significantly shorter than those of 1st and 3rd instar YSBLs on waxy maize (1st: 13.62 ± 1.22 d; 3rd: 13.67 ± 6.33 d) and sweet maize (1st: 16.00 ± 1.79 d; 3rd: 18.00 ± 3.49 d) at 30 °C. The body lengths of male and female YSBLs on wheat (male: 29.52 ± 0.40 mm, female: 34.97 ± 0.45 mm) and rice (male: 28.85 ± 0.68 mm, female: 34.66 ± 0.35 mm) were significantly longer than those observed when they were fed on sweet maize (male: 25.64 ± 1.60 mm, female: 21.93 ± 6.89 mm). There were only male adults obtained on waxy maize. The phenotypic characteristics of the YSBLs feeding on rice seedlings were very close to those of the YSBLs feeding on wheat seedlings. A relatively slower decline was observed in the survival rates of YSBL nymphs on wheat and rice compared to those on waxy maize and sweet maize at 25 °C, 30 °C, and 35 °C. In short, this study implied that YSBLs prefer wheat and rice. This study is the first report of direct damage caused by the YSBL to wheat in the laboratory, and its results could be useful in improving our understanding of the host preference of the YSBL and providing strategies for the management of this pest in field crops.
Self-DNA Inhibition in Drosophila melanogaster Development: Metabolomic Evidence of the Molecular Determinants
Authors: Colombo Michele , Grauso Laura , Lanzotti Virginia , Incerti Guido , Adamo Adele , Storlazzi Aurora , Gigliotti Silvia , Mazzoleni Stefano
Published: October 2023

We investigated the effects of dietary delivered self-DNA in the model insect Drosophila melanogaster. Self-DNA administration resulted in low but significant lethality in Drosophila larvae and considerably extended the fly developmental time. This was characterized by the abnormal persistence of the larvae in the L2 and L3 stages, which largely accounted for the average 72 h delay observed in pupariation, as compared to controls. In addition, self-DNA exposure affected adult reproduction by markedly reducing both female fecundity and fertility, further demonstrating its impact on Drosophila developmental processes. The effects on the metabolites of D. melanogaster larvae after exposure to self-DNA were studied by NMR, LC-MS, and molecular networking. The results showed that self-DNA feeding reduces the amounts of all metabolites, particularly amino acids and N-acyl amino acids, which are known to act as lipid signal mediators. An increasing amount of phloroglucinol was found after self-DNA exposure and correlated to developmental delay and egg-laying suppression. Pidolate, a known intermediate in the γ-glutamyl cycle, also increased after exposure to self-DNA and correlated to the block of insect oogenesis.
The Oxidative Stress and Nervous Distress Connection in Gastrointestinal Disorders
Authors: Stavely Rhian , Ott Leah C. , Rashidi Niloufar , Sakkal Samy , Nurgali Kulmira
Published: October 2023

Oxidative stress is increasingly recognized as a central player in a range of gastrointestinal (GI) disorders, as well as complications stemming from therapeutic interventions. This article presents an overview of the mechanisms of oxidative stress in GI conditions and highlights a link between oxidative insult and disruption to the enteric nervous system (ENS), which controls GI functions. The dysfunction of the ENS is characteristic of a spectrum of disorders, including neurointestinal diseases and conditions such as inflammatory bowel disease (IBD), diabetic gastroparesis, and chemotherapy-induced GI side effects. Neurons in the ENS, while essential for normal gut function, appear particularly vulnerable to oxidative damage. Mechanistically, oxidative stress in enteric neurons can result from intrinsic nitrosative injury, mitochondrial dysfunction, or inflammation-related pathways. Although antioxidant-based therapies have shown limited efficacy, recognizing the multifaceted role of oxidative stress in GI diseases offers a promising avenue for future interventions. This comprehensive review summarizes the literature to date implicating oxidative stress as a critical player in the pathophysiology of GI disorders, with a focus on its role in ENS injury and dysfunction, and highlights opportunities for the development of targeted therapeutics for these diseases.
Additive Anticonvulsant Profile and Molecular Docking Analysis of 5,5′-Diphenylhydantoin Schiff Bases and Phenytoin
Authors: Tchekalarova Jana , Todorov Petar , Rangelov Miroslav , Stoyanova Tsveta , Todorova Nadezhda
Published: October 2023

Four 5,5′-diphenylhydantoin Schiff bases possessing different aromatic species (SB1–SB4) were recently synthesized and characterized using spectroscopic and electrochemical tools. The present study aimed to ascertain the anticonvulsant activity of the novel phenytoin derivatives SB1-Ph, SB2-Ph, SB3-Ph, and SB4-Ph, containing different electron-donor and electron-acceptor groups, and their possible mechanism of action. The SB2-Ph exhibited the highest potency to suppress the seizure spread with ED50 = 8.29 mg/kg, comparable to phenytoin (ED50 = 5.96 mg/kg). While SB2-Ph did not produce neurotoxicity and sedation, it decreased locomotion and stereotypy compared to control. When administered in combination, the four Schiff bases decreased the phenytoin ED50 by more than 2× and raised the protective index by more than 7× (phenytoin+SB2-Ph). The strongest correlation between in-vivo and docking study results was found for ligands’ interaction energies with kappa and delta receptors. These data, combined with the worst interaction energies of our ligands with the mu receptor, suggest that the primary mechanism of their action involves the kappa and delta receptors, where the selectivity to the kappa receptor leads to higher biological effects. Our findings suggest that the four Schiff bases might be promising candidates with potential applications as a safe and effective adjuvant in epilepsy.
Herbal Compounds Dauricine and Isoliensinine Impede SARS-CoV-2 Viral Entry
Authors: Dabrell Shaneek Natoya , Li Yi-Chuan , Yamaguchi Hirohito , Chen Hsiao-Fan , Hung Mien-Chie
Published: October 2023

Targeting viral entry has been the focal point for the last 3 years due to the continued threat posed by SARS-CoV-2. SARS-CoV-2’s entry is highly dependent on the interaction between the virus’s Spike protein and host receptors. The virus’s Spike protein is a key modulator of viral entry, allowing sequential cleavage of ACE2 at the S1/S2 and S2 sites, resulting in the amalgamation of membranes and subsequent entry of the virus. A Polybasic insertion (PRRAR) conveniently located at the S1/S2 site can also be cleaved by furin or by serine protease, TMPRSS2, at the cell surface. Since ACE2 and TMPRSS2 are conveniently located on the surface of host cells, targeting one or both receptors may inhibit receptor-ligand interaction. Here, we show that Dauricine and Isoliensinine, two commonly used herbal compounds, were capable of inhibiting SARS-CoV-2 viral entry by reducing Spike-ACE2 interaction but not suppressing TMPRSS2 protease activity. Further, our biological assays using pseudoviruses engineered to express Spike proteins of different variants revealed a reduction in infection rates following treatment with these compounds. The molecular modeling revealed an interconnection between R403 of Spike protein and both two compounds. Spike mutations at residue R403 are critical, and often utilized by ACE2 to gain cell access. Overall, our findings strongly suggest that Dauricine and Isoliensinine are effective in blocking Spike-ACE2 interaction and may serve as effective therapeutic agents for targeting SARS-CoV-2′s viral entry.
Physical-Chemical and Microhardness Properties of Model Dental Composites Containing 1,2-Bismethacrylate-3-eugenyl Propane Monomer
Authors: Al-Odayni Abdel-Basit , Al-Kahtani Haifa Masfeer , Sharaf Saeed Waseem , Al-Kahtani Abdullah , Aouak Taieb , Khan Rawaiz , De Vera Merry Angelyn Tan , Alrahlah Ali
Published: October 2023

A new eugenyl dimethacrylated monomer (symbolled BisMEP) has recently been synthesized. It showed promising viscosity and polymerizability as resin for dental composite. As a new monomer, BisMEP must be assessed further; thus, various physical, chemical, and mechanical properties have to be investigated. In this work, the aim was to investigate the potential use of BisMEP in place of the BisGMA matrix of resin-based composites (RBCs), totally or partially. Therefore, a list of model composites (CEa0, CEa25, CEa50, and CEa100) were prepared, which made up of 66 wt% synthesized silica fillers and 34 wt% organic matrices (BisGMA and TEGDMA; 1:1 wt/wt), while the novel BisMEP monomer has replaced the BisGMA content as 0.0, 25, 50, and 100 wt%, respectively. The RBCs were analyzed for their degree of conversion (DC)-based depth of cure at 1 and 2 mm thickness (DC1 and DC2), Vickers hardness (HV), water uptake (WSP), and water solubility (WSL) properties. Data were statistically analyzed using IBM SPSS v21, and the significance level was taken as p < 0.05. The results revealed no significant differences (p > 0.05) in the DC at 1 and 2 mm depth for the same composite. No significant differences in the DC between CEa0, CEa25, and CEa50; however, the difference becomes substantial (p < 0.05) with CEa100, suggesting possible incorporation of BisMEP at low dosage. Furthermore, DC1 for CEa0–CEa50 and DC2 for CEa0–CEa25 were found to be above the proposed minimum limit DC of 55%. Statistical analysis of the HV data showed no significant difference between CEa0, CEa25, and CEa50, while the difference became statistically significant after totally replacing BisGMA with BisMEP (CEa100). Notably, no significant differences in the WSP of various composites were detected. Likewise, WSL tests revealed no significant differences between such composites. These results suggest the possible usage of BisMEP in a mixture with BisGMA with no significant adverse effect on the DC, HV, WSP, and degradation (WSL).
Multiplex Portable Biosensor for Bacteria Detection
Authors: Kaci Karim , Enebral-Romero Estefanía , Martínez-Periñán Emiliano , Garrido Marina , Pérez Emilio M. , López-Diego David , Luna Mónica , González-de-Rivera Guillermo , García-Mendiola Tania , Lorenzo Encarnación
Published: October 2023

An advanced, cost-effective, and portable DNA biosensor capable of detecting multiple bacteria simultaneously has been developed. The biosensor comprises a fast and inexpensive potentiostat that controls the applied potential to a screen-printed electrochemical array platform functionalized with MoS2 flakes and bacterial DNA probes. The current response obtained by à la carte thionine functionalized carbon nanodots (Ty-CDs) is monitored as an electrochemical indicator of the hybridization event. The design of the potentiostat prioritizes achieving an optimal signal-to-noise ratio and incorporates a user-friendly interface compatible with various devices, including computers, mobile phones, and tablets. The device is compact, lightweight, and manufactured at a low cost. The key components of the potentiostat include a data acquisition board capable of analyzing multiple samples simultaneously and a controller board. The results of this study confirm the ability of the multiplex portable biosensor to successfully detect specific bacterial DNA sequences, demonstrating its reliability and superior performance compared with a traditional, more complex, and laboratory-oriented potentiostat.
Complementary Methodology for Energy Efficiency Ratio-Based Assessments with Change-Point Model Parameters
Authors: Kim Hye Gi , Kim Sun Sook
Published: October 2023

The energy consumption of existing buildings is not only affected by their physical features but also by their business activities (e.g., operating hours, number of workers, and climate). Energy Star’s energy efficiency ratio (EER) is a key energy performance indicator that has been used for more than 20 years. This method normalizes operation characteristics by calculating the estimated energy consumption of business activities using regression models and comparing it with the actual energy consumption. However, EER-based assessment is limited by the lack of information regarding the reasons for the evaluation results. This study proposes a balanced method for explaining the reasons underlying energy efficiency levels while maintaining the existing EER assessment system. The method constitutes data collection, EPI derivation, and energy performance assessment, utilizing the parameters of the change-point linear model (CPM) as an additional EPI to provide descriptive information. The results are summarized to provide a checklist guide for retrofitting, and additional energy saving potential for buildings with low and high scores can be identified by comparing the EER scores and CPM parameters. The proposed method shows that it is possible to interpret the energy efficiency assessment results by comparing CPM parameters, while maintaining the EER score.
The Role of the Microenvironment and Cell Adhesion Molecules in Chronic Lymphocytic Leukemia
Authors: Cerreto Marina , Foà Robin , Natoni Alessandro
Published: October 2023

Chronic lymphocytic leukemia (CLL) is a B-cell malignancy whose progression largely depends on the lymph node and bone marrow microenvironment. Indeed, CLL cells actively proliferate in specific regions of these anatomical compartments, known as proliferation centers, while being quiescent in the blood stream. Hence, CLL cell adhesion and migration into these protective niches are critical for CLL pathophysiology. CLL cells are lodged in their microenvironment through a series of molecular interactions that are mediated by cellular adhesion molecules and their counter receptors. The importance of these adhesion molecules in the clinic is demonstrated by the correlation between the expression levels of some of them, in particular CD49d, and the prognostic likelihood. Furthermore, novel therapeutic agents, such as ibrutinib, impair the functions of these adhesion molecules, leading to an egress of CLL cells from the lymph nodes and bone marrow into the circulation together with an inhibition of homing into these survival niches, thereby preventing disease progression. Several adhesion molecules have been shown to participate in CLL adhesion and migration. Their importance also stems from the observation that they are involved in promoting, directly or indirectly, survival signals that sustain CLL proliferation and limit the efficacy of standard and novel chemotherapeutic drugs, a process known as cell adhesion-mediated drug resistance. In this respect, many studies have elucidated the molecular mechanisms underlying cell adhesion-mediated drug resistance, which have highlighted different signaling pathways that may represent potential therapeutic targets. Here, we review the role of the microenvironment and the adhesion molecules that have been shown to be important in CLL and their impact on transendothelial migration and cell-mediated drug resistance. We also discuss how novel therapeutic compounds modulate the function of this important class of molecules.