bluuebunny/crossref_metadata_2025 huggingface dataset:
Applications of Disaffinity Vectors to Certain Riemannian Manifolds
Authors: Alohali Hanan , Deshmukh Sharief , Chen Bang-Yen
Published: December 2024

A disaffinity vector on a Riemannian manifold is a vector field whose affinity tensor vanishes. In this paper, we prove that every disaffinity vector on a compact Riemannian manifold is an incompressible vector field. Then, we discover a sufficient condition for an incompressible vector field to be disaffinity. Next, we study trans-Sasakian 3-manifolds whose Reeb vector field is disaffinity and obtain two sufficient conditions for a trans-Sasakian 3-manifold to be homothetic to a Sasakian 3-manifold. Finally, we prove that a complete Riemannian manifold admitting a non-harmonic disaffinity function satisfying the Eikonal equation and a Ricci curvature inequality is isometric to a Euclidean space.
On (i)-Curves in Blowups of Pr
Authors: Dumitrescu Olivia , Miranda Rick
Published: December 2024

In this paper, we study (i)-curves with i∈{−1,0,1} in the blown-up projective space Pr in general points. The notion of (−1)-curves was analyzed in the early days of mirror symmetry by Kontsevich, with the motivation of counting curves on a Calabi–Yau threefold. In dimension two, Nagata studied planar (−1)-curves in order to construct a counterexample to Hilbert’s 14th problem. We introduce the notion of classes of (0)- and (1)-curves in Pr with s points blown up, and we prove that their number is finite if and only if the space is a Mori Dream Space. We further introduce a bilinear form on a space of curves and a unique symmetric Weyl-invariant class, F (which we will refer to as the anticanonical curve class). For Mori Dream Spaces, we prove that (−1)-curves can be defined arithmetically by the linear and quadratic invariants determined by the bilinear form. Moreover, (0)- and (1)-Weyl lines give the extremal rays for the cone of movable curves in Pr with r+3 points blown up. As an application, we use the technique of movable curves to reprove that if F2≤0 then Y is not a Mori Dream Space, and we propose to apply this technique to other spaces.
New Metaheuristics to Solve the Internet Shopping Optimization Problem with Sensitive Prices
Authors: García-Morales Miguel A. , Brambila-Hernández José Alfredo , Fraire-Huacuja Héctor J. , Frausto Juan , Cruz Laura , Gómez Claudia , Peña-Ramos Alfredo
Published: December 2024

In this research, two new methods for solving the Internet shopping optimization problem with sensitive prices are proposed, incorporating adaptive adjustment of control parameters. This problem is classified as NP-hard and is relevant to current electronic commerce. The first proposed solution method corresponds to a Memetic Algorithm incorporating improved local search and adaptive adjustment of control parameters. The second proposed solution method is a particle swarm optimization algorithm that adds a technique for diversification and adaptive adjustment of control parameters. We assess the effectiveness of the proposed algorithms by comparing them with the Branch and Bound algorithm, which presents the most favorable outcomes of the state-of-the-art method. Nine instances of three different sizes are used: small, medium, and large. For performance validation, the Wilcoxon and Friedman non-parametric tests are applied. The results show that the proposed algorithms exhibit comparable performance and outperform the Branch and Bound algorithm.
Three New Dipeptide and Two New Polyketide Derivatives from the Mangrove-Derived Fungus Talaromyces sp.: Antioxidant Activity of Two Isolated Substances
Authors: Zeng Zhihao , Cai Jian , Chen Yi , Li Xinlong , Chen Chunmei , Liu Yonghong , Jayasinghe Lalith , Zhou Xuefeng
Published: December 2024

Five new metabolites, including three cyclic dipeptide derivatives (1–3) and two new polyketides (10–11), together with nine known ones (4–9 and 12–15), were isolated from the mangrove-sediments-derived fungus Talaromyces sp. SCSIO 41431. Their structures were determined using detailed NMR, MS spectroscopic analyses, and quantum chemical calculations. X-ray single-crystal diffraction analysis of 1 was described. Compounds 13–15 demonstrated activity against Staphylococcus aureus, with MIC values ranging from 25 to 50 µg/mL. Compound 9 showed activity against Escherichia coli, Streptococcus suis, and Erysipelothrix rhusiopathiae, with an MIC value of 100 µg/mL. In addition, compounds 1 and 12 showed DPPH radical scavenging activity, with the EC50 of 27.62 and 29.34 µg/mL, compared to the positive control (ascorbic acid, EC50, 12.74 µg/mL).
Interaction of Liposomes Containing the Carrageenan/Echinochrome Complex with Human HaCaT Keratinocytes In Vitro
Authors: Menchinskaya Ekaterina S. , Gorbach Vladimir I. , Pislyagin Evgeny A. , Gorpenchenko Tatiana Y. , Pimenova Evgeniya A. , Guzhova Irina V. , Aminin Dmitry L. , Yermak Irina M.
Published: December 2024

Liposomal drug delivery systems are successfully used in various fields of medicine for external and systemic applications. Marine organisms contain biologically active substances that have a unique structure and exhibit a wide range of biological activities. Polysaccharide of red seaweed (carrageenan (CRG)), and water-insoluble sea urchin pigment (echinochrome (Ech)) interact with each other and form a stable complex. We included the CRG/Ech complex in liposomes for better permeability into cells. In our research, tetramethylrhodamine isothiocyanate TRITC-labeled CRG was synthesized to study the interaction of the complex encapsulated in liposomes with human epidermal keratinocytes (HaCaTs) widely used to expose the skin to a variety of substances. Using confocal microscopy, we found that liposomes were able to penetrate HaCaT cells with maximum efficiency within 24 h, and pre-incubation of keratinocytes with liposomes resulted in the delivery of the CRG/Ech complex into the cytoplasm. We investigated the anti-inflammatory effects of liposomes, including the lysosomal regulation, increased intracellular ROS levels, and increased NO synthesis in lipopolysaccharide (LPS)- or Escherichia coli (E. coli)-induced inflamed skin cells. Liposomes containing the CRG/Ech complex significantly reduced lysosomal activity by 26% in LPS-treated keratinocytes and decreased ROS levels in cells by 23% after LPS exposure. It was found that liposomes with the complex improved the migration of HaCaT keratinocytes incubated with high-dose LPS by 47%. The results of the work, taking into account the good permeability of liposomes into keratinocytes, as well as the anti-inflammatory effect on cells treated with LPS or E. coli, show the prospects of using liposomes containing the CRG/Ech complex as an anti-inflammatory agent in the fight against skin infections.
Religious Fasting Following Metabolic and Bariatric Surgery (MBS): Insights from Jewish Practices in Israel
Authors: Eldar Shai Meron , Keidar Andrei , Abu-Abeid Adam ,   on behalf of the ISMBS Collaborative Group
Published: December 2024

Background and Objectives: Religious fasting in patients after Metabolic and Bariatric Surgery (MBS) remains a topic with limited clarity. This study aims to present the results of a survey on religious fasting in patients after MBS in Israel. The questionnaire was sent to members of the Israeli Society for Metabolic and Bariatric Surgery (ISMBS). Materials and Methods: An online questionnaire survey was designed and distributed to members of the ISMBS. The survey consisted of 23 questions addressing religious fasting in patients after MBS and was divided into three sections: (1) MBS surgeon clinical experience, (2) clinical considerations regarding religious fasting in MBS patients, and (3) fasting-related complications in MBS patients. Responses were recorded and presented as numbers (percentages), with results analyzed descriptively and/or graphically. Results: The ISMBS has 63 active members, and 37 members (59%) responded to the survey. Most respondents have more than 10 years of MBS experience and perform more than 100 MBS procedures annually (67.5% and 54%, respectively). In general, 81.1% of respondents permit religious fasting in patients after MBS, and 73% think that fasting could be safe at least 12 months after MBS. Most (62.2%) agree that a clinical evaluation should be undertaken prior to permitting religious fasting; 40% of respondents note that there is increased patient admission to emergency rooms during religious fasting, mostly due to dehydration. When asked about fasting risks, most noted hypoglycemia (40.5%) and the evolution of marginal ulcers (16.2%). Conclusions: In conclusion, these national survey results emphasize the variations in MBS surgeons’ opinions regarding religious fasting after MBS. Despite these differences, there were still many similarities in responses such as timing and fasting permission, and this study could aid clinicians in the future when consulted on religious fasting by MBS patients.
The Perceived Effectiveness of Cannabidiol on Adult Women with Inflammatory Bowel Disease
Authors: Portman Ayelet , Bukovich Emily , Bissex Janice , Flanagan Molly , Pojednic Rachele
Published: December 2024

Background and Objectives: In this study we analyzed the perceived effectiveness of cannabidiol (CBD) in adult women with inflammatory bowel disease (IBD). Materials and Methods: We conducted an online cross-sectional survey which assessed disease severity and quality of life (QOL) in women with IBD (Crohn’s disease and ulcerative colitis) who used CBD versus those who did not. The survey included: the Harvey–Bradshaw Index, Partial Mayo Score, Short Inflammatory Bowel Disease Questionnaire, Bristol Stool Scale, and the Prime Screen Short Food Frequency Questionnaire. CBD utilization practices were also examined. STROBE reporting outcomes were followed. Statistical methods included Pearson and Spearman’s correlations and chi-square analyses. Results: Seventy-one (n = 71) women were included. Twenty-five (n = 25) were CBD users and forty-six (n = 46) were past/never users. Most current users ingested CBD once per day (40%), acquired CBD from a dispensary (56%), and utilized an oral tincture (40%) at a dose of 25 mg or less (56%). There was no statistical association between CBD use and disease severity, quality of life (QOL), fecal consistency, or food intake. However, CBD users reported decreases in IBD-related pain and nausea (76% and 64%, respectively), and improvement in appetite (60%). Overall, disease severity and QOL were inversely correlated (past/never users: p = 0.000, r = −0.544; current users: p = 0.042, r = −0.427). Conclusions: Women with IBD who use CBD insignificantly trended toward improved disease-related symptoms, appetite, and QOL compared to non-users. Disease severity and QOL were inversely associated, regardless of CBD use. These preliminary outcomes indicate the need for further research on CBD use in women with IBD.
Clinical and Molecular Impact of Advanced Platelet-Rich Fibrin on Pain, Swelling, and Distal Periodontal Status of Mandibular Second Molars After Mandibular Third-Molar Extraction
Authors: Stefanescu Ada , Sufaru Irina-Georgeta , Chiscop Iulia , Lupu Fabian Cezar , Martu Cristian , Oprisan Bogdan , Earar Kamel
Published: December 2024

Background and Objectives: This study aimed to evaluate the role of A-PRF (advanced platelet-rich fibrin) in the enhancement of wound healing and protecting the periodontal health of mandibular second molars after the extraction of mandibular third molars. Additionally, the study assessed the levels of pro-inflammatory cytokines in the gingival crevicular fluid (GCF) of mandibular second molars as markers of inflammation. Materials and Methods: Twenty-five systemically healthy adult patients with bilateral removal of impacted mandibular third molars were included. Each patient received A-PRF in one extraction site, while the contralateral site served as a control. Periodontal parameters of the adjacent second molar, including probing depth (PD) and clinical attachment level (CAL), were measured in distal–vestibular (DV) and distal–lingual (DL) sites. Pain, swelling, and overall healing were subjectively evaluated. Levels of tumor necrosis factor-alpha (TNF-α), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) in the GCF were analyzed. Evaluations occurred at baseline and three months post-surgery. Results: A-PRF significantly improved PD (from 4.69 ± 0.61 mm to 3.85 ± 0.34 mm in DV, and from 4.71 ± 0.65 mm to 3.79 ± 0.27 mm in DL, respectively) and CAL (from 2.41 ± 0.25 mm to 1.82 ± 0.21 mm in DV, and from 2.40 ± 0.36 mm to 1.75 ± 0.19 mm in DL, respectively) of the adjacent second molar, compared to control sites, three months post-surgery. Pain and swelling scores were notably lower on the 7th postoperative day in the A-PRF group. A-PRF also reduced pro-inflammatory cytokines in GCF, significantly more than in control sites, at three months post-surgery. Conclusions: A-PRF enhances the periodontal and inflammatory status of adjacent teeth and wound healing after the extraction of mandibular third molars.
Effects of Sacroiliac Joint Manipulation on Autonomic Nervous System and Lower Abdominal Pain in Women with Primary Dysmenorrhoea: A Randomized Controlled Trial
Authors: Park Sungeon , Kim Hyunjoong , Jung Jihye , Lee Seungwon
Published: December 2024

Background and Objectives: Autonomic nervous system (ANS) disorders are responsible for primary dysmenorrhea and are closely linked to the spine. This study aims to evaluate the effects of sacroiliac joint manipulation on the ANS and lower abdominal pain in women with primary dysmenorrhea and proposes an alternative treatment method. Materials and Methods: A total of 40 participants were randomly assigned; however, 35 participants remained in the final analysis after 5 dropped out; they were assigned to either the sacroiliac joint manipulation group (n = 18) or the superficial heat therapy group (n = 17). Assessments included heart rate variability (HRV), visual analogue scale (VAS) scores for lower abdominal pain, the pressure pain threshold (PPT) of the sacroiliac joint, and the Moos Menstrual Distress Questionnaire (MMDQ) at baseline and after 4 weeks. Only the VAS and MMDQ scores were reassessed after 8 weeks to evaluate the sustained effects of the treatment. Results: Changes in HRV showed improvements in ANS balance only in the sacroiliac joint manipulation group (p < 0.05). It was found to be more effective than superficial heat therapy (p < 0.05). A significant decrease in lower abdominal pain following the intervention was observed in both groups (p < 0.05), along with the sustained effects of the treatment (p < 0.05). The MMDQ scores decreased after sacroiliac joint manipulation (p < 0.05), with greater improvements compared to the superficial heat therapy (p < 0.05). Conclusions: Sacroiliac joint manipulation positively affects ANS balance and is effective in alleviating lower abdominal pain and menstruation-related symptoms, with sustained effects observed over time. Therefore, sacroiliac joint manipulation can be an effective alternative treatment for women with primary dysmenorrhea.
Trastuzumab Rechallenge in HER2-Positive Metastatic Breast Cancer: A Promising Strategy for Enhanced Progression-Free Survival Post Ado-Trastuzumab Emtansine Progression
Authors: Altıntaş Yunus Emre , Kınıkoğlu Oğuzcan , Yıldız Anıl , Işık Deniz , Özkerim Uğur , Öksüz Sıla , Tüylü Tuğba Başoğlu , Sürmeli Heves , Turan Nedim , Odabaş Hatice
Published: December 2024

Background and Objectives: Metastatic breast cancer (MBC), particularly the HER2-positive subtype, represents a significant clinical challenge, with approximately 20–25% of breast cancer cases demonstrating HER2 overexpression. Trastuzumab, a monoclonal antibody targeting HER2, has significantly improved outcomes in these patients. However, progression after second-line treatments such as trastuzumab emtansine (T-DM1) necessitates exploring subsequent therapeutic options. This study aims to compare the efficacy of trastuzumab plus gemcitabine (GT) with lapatinib plus capecitabine (LC) as third-line treatments in HER2-positive MBC post-T-DM1 failure. Materials and Methods: This retrospective study included 98 HER2-positive MBC patients treated between 2017 and 2023 who progressed after T-DM1. Patients were divided into two groups: 21 received GT, and 28 received LC. Key endpoints included progression-free survival (PFS), overall survival (OS), objective response rate (ORR), and adverse events. Statistical analyses were performed using SPSS 26.0, with Kaplan–Meier survival curves, log-rank tests, and Cox proportional hazards models. Results: Median PFS was significantly longer in the GT group (9.5 months) compared to the LC group (4.3 months, p = 0.02). OS was also higher for GT (22.1 months vs. 10.0 months, p = 0.02). ORR favored the GT group (33.3% vs. 10.7%, p = 0.046), and progressive disease was more common in the LC group (57.1% vs. 33.3%, p = 0.022). The safety profile showed higher rates of diarrhea in the LC group, but both regimens were generally well tolerated. Conclusions: Trastuzumab re-challenge with gemcitabine demonstrated superior PFS, OS, and ORR compared to lapatinib plus capecitabine, suggesting it may be a more effective third-line therapy in HER2-positive MBC patients who have progressed after T-DM1. Further prospective studies are needed to confirm these findings and optimize treatment sequencing.
Prognostic Value of Immune Scoring System for Colorectal Cancer Patients with Peritoneal Metastasis
Authors: Manoğlu Berke , Sökmen Selman , Egeli Tufan , Derici Zekai Serhan , Ağalar Cihan , Aksoy Süleyman Özkan
Published: December 2024

Background and Objectives: There is no reliable immune scoring system that can help us predict the postoperative outcomes of colorectal cancer patients with peritoneal metastases after cytoreductive surgery. In this cohort, the aims were (1) to evaluate the postoperative morbidity, mortality and surgical oncological outcomes in colorectal cancer patients with peritoneal metastasis; (2) to compare oncological and postoperative outcomes of colon cancer patients with peritoneal metastasis and rectal cancer patients with peritoneal metastasis; and (3) to assess the prognostic value of the modified Glasgow Prognostic Score (mGPS) and the CRP–albumin ratio (CAR). Materials and Methods: A prospectively maintained database of 258 patients who underwent cytoreductive surgery for peritoneal metastases of colorectal origin between 2007 and 2024 was analyzed. According to the anatomical location of the primary tumor, two different groups were created: rectum cancer patients with peritoneal metastasis (Group A) and colon cancer patients with peritoneal metastasis (Group B). All standard clinico-pathological characteristics, operative findings, morbi-mortality results, and final oncologic outcomes were compared between Groups A and B. We evaluated whether CAR and mGPS could predict postoperative morbi-mortality and overall survival in the two groups or not. Results: No significant difference was detected between Groups A and B in terms of clinical–demographic characteristics. In both groups, the preoperative mGPS and CAR values were statistically significantly higher in those who developed postoperative high-grade complications (C-D grade III/IV) (p < 0.001) and those who died perioperatively (p = 0.001 and p = 0.002). Conclusions: In multivariate Cox analysis, the CAR was found to be an independent prognostic factor for overall survival in this cohort. CAR and mGPS predicted high-grade complications and postoperative mortality in both groups.
Colliding Challenges Part 2: An Analysis of SARS-CoV-2 Infection in Patients with Extrapulmonary Tuberculosis Versus SARS-CoV-2 Infection Alone
Authors: Mihuta Camil , Socaci Adriana , Hogea Patricia , Tudorache Emanuela , Mihuta Monica Simina , Oancea Cristian
Published: December 2024

Background and Objectives: Coinfection with SARS-CoV-2 and extrapulmonary tuberculosis (extraPTB) presents unique clinical challenges due to dual inflammatory responses and potential differences in patient profiles compared to those with SARS-CoV-2 infection alone. This study uniquely contributes to the underexplored interaction between extraPTB and SARS-CoV-2, focusing on systemic inflammation as a critical determinant of outcomes. Materials and Methods: This retrospective, cross-sectional study included 123 patients aged 19–91 years, hospitalized at Victor Babeș Hospital in Timișoara from March 2020 to March 2022. We compared 23 extraPTB and SARS-CoV-2 coinfected patients with 100 age-matched SARS-CoV-2-only patients. Clinical records were examined for demographic, clinical, and laboratory data. Results: The coinfected group was younger, with 65% under 40 years, and presented significantly higher IL-6, PCT, and transaminase levels. Coexisting COPD and type 2 diabetes were independent predictors of coinfection. A higher SpO2 at diagnosis was positively associated with coinfection likelihood (OR = 5.37), while CT scores indicated less pulmonary involvement in coinfected patients. Non-fatal outcomes were more frequent in the coinfection group (95.7% sensitivity), and only one coinfected patient had a fatal outcome versus 17 in the SARS-CoV-2-only group. Low SpO2 and elevated IL-6 were significant predictors of mortality, with severe symptoms tripling fatality odds. Conclusions: Coinfection with extraPTB and SARS-CoV-2 is associated with younger age, heightened systemic inflammation, and longer hospital stays but does not significantly increase mortality risk compared to SARS-CoV-2 alone. These findings underscore the importance of monitoring systemic inflammatory markers and developing tailored management strategies to improve long-term care outcomes for coinfected patients, especially in resource-limited settings.
Microstructure, Mechanical, and Tribological Behaviour of Spark Plasma Sintered TiN, TiC, TiCN, TaN, and NbN Ceramic Coatings on Titanium Substrate
Authors: Walunj Ganesh , Choudhari Amit , Digole Satyavan , Bearden Anthony , Kolt Omar , Bari Praful , Borkar Tushar
Published: December 2024

Titanium (Ti) is widely used in structural, maritime, aerospace, and biomedical applications because of its outstanding strength-to-weight ratio, superior corrosion resistance, and excellent biocompatibility. However, the lower surface hardness and inferior wear resistance of the Ti and Ti alloys limit their industrial applications. Coating Ti surfaces can initiate new possibilities to give unique characteristics with significant improvement in the Ti component’s functionality. The current research designed and synthesized titanium nitride (TiN), titanium carbide (TiC), titanium carbonitride (TiCN), tantalum nitride (TaN), and niobium nitride (NbN) ceramic coating layers (400 µm) over a Ti substrate using a spark plasma sintering process (SPS). The coatings on the Ti substrate were compact and consolidated at an SPS temperature of 1500 °C, pressure of 50 MPa, and 5 min of holding time in a controlled argon atmosphere. Microstructure investigation revealed a defect-less coating-substrate interface formation with a transition/diffusion zone ranging from 10 µm to 20 µm. Among all of the ceramic coatings, titanium carbide showed the highest improvement in surface hardness, equal to 1817 ± 25 HV, and the lowest coefficient of friction, equal to 0.28 for NbN.
Deep Eutectic Solvent (TOPO/D2EHPA/Menthol) for Extracting Metals from Synthetic Hydrochloric Acid Leachates of NMC-LTO Batteries
Authors: Kozhevnikova Arina V. , Milevskii Nikita A. , Lobovich Dmitriy V. , Zakhodyaeva Yulia A. , Voshkin Andrey A.
Published: December 2024

The recycling of lithium-ion batteries is increasingly important for both resource recovery and environmental protection. However, the complex composition of cathode and anode materials in these batteries makes the efficient separation of metal mixtures challenging. Hydrometallurgical methods, particularly liquid extraction, provide an effective means of separating metal ions, though they require periodic updates to their extraction systems. This study introduces a hydrophobic deep eutectic solvent composed of trioctylphosphine oxide, di(2-ethylhexyl)phosphoric acid, and menthol, which is effective for separating Ti(IV), Co(II), Mn(II), Ni(II), and Li+ ions from hydrochloric acid leachates of NMC (LiNixMnyCo1−x−yO2) batteries with LTO (Li4Ti5O12) anodes. By optimising the molar composition of the trioctylphosphine oxide/di(2-ethylhexyl)phosphoric acid/menthol mixture to a 4:1:5 ratio, high extraction efficiency was achieved. The solvent demonstrated stability over 10 cycles, and conditions for its regeneration were successfully established. At room temperature, the DES exhibited a density of 0.89 g/mL and a viscosity of 56 mPa·s, which are suitable for laboratory-scale extraction processes. Experimental results from a laboratory setup with mixer-settlers confirmed the efficiency of separating Ti(IV) and Co(II) ions in the context of their extraction kinetics.
A Comprehensive Metabolomic and Microbial Analysis Following Dietary Amino Acid Reduction in Mice
Authors: Al-Ishaq Raghad Khalid , Ferrara Carmen R. , Stephan Nisha , Krumsiek Jan , Suhre Karsten , Montrose David C.
Published: December 2024

Introduction: Nutritional metabolomics provides a comprehensive overview of the biochemical processes that are induced by dietary intake through the measurement of metabolite profiles in biological samples. However, there is a lack of deep phenotypic analysis that shows how dietary interventions influence the metabolic state across multiple physiologic sites. Dietary amino acids have emerged as important nutrients for physiology and pathophysiology given their ability to impact cell metabolism. Methods: The aim of the current study is to evaluate the effect of modulating amino acids in diet on the metabolome and microbiome of mice. Here, we report a comprehensive metabolite profiling across serum, liver, and feces, in addition to gut microbial analyses, following a reduction in either total dietary protein or diet-derived non-essential amino acids in mice. Results: We observed both distinct and overlapping patterns in the metabolic profile changes across the three sample types, with the strongest signals observed in liver and serum. Although amino acids and related molecules were the most commonly and strongly altered group of metabolites, additional small molecule changes included those related to glycolysis and the tricarboxylic acid cycle. Microbial profiling of feces showed significant differences in the abundance of select species across groups of mice. Conclusions: Our results demonstrate how changes in dietary amino acids influence the metabolic profiles across organ systems and the utility of metabolomic profiling for assessing diet-induced alterations in metabolism.
Arbuscular Mycorrhizal Fungi as a Salt Bioaccumulation Mechanism for the Establishment of a Neotropical Halophytic Fern in Saline Soils
Authors: Lugo Mónica A. , Negritto María A. , Crespo Esteban M. , Iriarte Hebe J. , Núñez Samuel , Espinosa Luisa F. , Pagano Marcela C.
Published: December 2024

Acrostichum aureum is a halophytic pantropical invasive fern growing in mangroves and swamps. Its association with arbuscular mycorrhizal fungi (AMF) has been reported in Asia. AMF and their symbiosis (AM) commonly colonise the absorption organs of terrestrial plants worldwide. Furthermore, AMF/AM are well known for their capacity to bioaccumulate toxic elements and to alleviate biotic and abiotic stress (e.g., salinity stress) in their hosts. However, the mechanisms underlying AMF involvement in the halophytism of A. aureum and the structures where NaCl accumulates remain unknown. This study shows that A. aureum forms AM in margins of natural thermal ponds in Neotropical wetlands. All mature sporophytes were colonised by AMF, with high percentages for root length (ca. 57%), arbuscules (23), and hyphae (25) and low values for vesicles (2%). In A. aureum–AMF symbiosis, NaCl accumulated in AMF vesicles, and CaSO4 precipitated in colonised roots. Therefore, AM can contribute to the halophytic nature of this fern, allowing it to thrive in saline and thermal environments by capturing NaCl from fern tissues, compartmentalising it inside its vesicles, and precipitating CaSO4.
Low 25-Hydroxyvitamin D Post-Kidney Transplant Is Associated with Increased Risk of BK Polyomavirus-Associated Nephropathy
Authors: Raj Suseela A. , Zhou Angela L. , Fedorova Ekaterina , Yuan Zhongyu , Mandelbrot Didier A. , Astor Brad C. , Parajuli Sandesh
Published: December 2024

BK viremia (BKPyV-DNAemia) and nephropathy (BKPyVAN) are significant causes of morbidity and mortality in kidney transplant recipients (KTRs). Vitamin D supports immune function, yet low 25-hydroxyvitamin D [25(OH)D] is common among KTRs. The association between serum 25(OH)D, measured 61 days to 2 years post-transplant, and subsequent incident BKPyV-DNAemia and BKPyVAN was examined in KTRs without previous BKPyV-DNAemia or BKPyVAN, respectively. Out of 3308 KTRs, 399 (12%) were vitamin D deficient [25(OH)D ≤ 20 ng/mL], and 916 (27.7%) were insufficient [25(OH)D 21–29 ng/mL]. A total of 184 KTRs developed BKPyV-DNAemia and 44 developed BKPyVAN. The incidence rate (/100 person-years) for BKPyV-DNAemia was 2.88 in the 25(OH)D sufficient group, 2.22 in the insufficient group, and 2.37 in the deficient group. The incidence rate (/100 person-years) for BKPyVAN was 0.30 in the 25(OH)D sufficient group, 0.75 in the insufficient group, and 1.28 in the deficient group. Vitamin D deficiency (adjusted hazard ratio [aHR] compared to 25(OH)D sufficiency: 3.92; 95% CI: 1.66–9.23) and insufficiency (aHR: 2.22; 95% CI: 1.11–4.45) remained significantly associated with the incidence of BKPyVAN after adjustment for baseline characteristics. Low serum 25(OH)D was associated with an increased risk of BKPyVAN but not BKPyV-DNAemia.
Investigating How Genomic Contexts Impact IS5 Transposition Within the Escherichia coli Genome
Authors: Onstead Jonathan , Zhang Zhongge , Huo Jialu , Ord Jack W. , Smith Sofia , Saier Milton H.
Published: December 2024

Insertions of the transposable element IS5 into its target sites in response to stressful environmental conditions, DNA structures, and DNA-binding proteins are well studied, but how the genomic contexts near IS5′s native loci impact its transpositions is largely unknown. Here, by examining the roles of all 11 copies of IS5 within the genome of E. coli strain BW25113 in transposition, we reveal that the most significant copy of IS5 is one nested within and oriented in the same direction as the nmpC gene, while two other copies of IS5 harboring point mutations are hardly transposed. Transposition activity is heavily reliant on the upstream nmpC promoter that drives IS5 transposase gene ins5A, with more transpositions resulting from greater promoter activity. The IS5 element at nmpC but not at other loci transcribed detectable amounts of ins5A mRNA. By increasing expression of the ins5CB operon harbored in IS5, we demonstrate that Ins5B and Ins5C appear to exert a stimulatory role in IS5 transposition, suggesting that the downstream genomic regions near the native loci are involved in overall IS5 transposition as well. Using a strain that carries IS5 only at the nmpC locus, we confirm that IS5 primarily uses a copy/paste mechanism for transposition, although we cannot rule out the cut/paste mechanism.
Recent Developments in Thermally Stable Transparent Thin Films for Heater Applications: A Systematic Review
Authors: Traiwattanapong Worawat , Molahalli Vandana , Pattanaporkratana Apichart , Chattham Nattaporn
Published: December 2024

Transparent thin-film heaters have sparked great interest in both the scientific and industrial sectors due to their critical role in various technologies, including smart windows, displays, actuators, and sensors. In this review, we summarize the structural design, fabrication methods, properties, and materials used in thin-film heaters. We also discuss methods to improve their efficiency and recent advancements in the field, and provide insights into the market size, growth, and future outlook for thin-film heaters.
Impact of Gas Diffusion Layer Compression on Electrochemical Performance in Proton Exchange Membrane Fuel Cells: A Three-Dimensional Lattice Boltzmann Pore-Scale Analysis
Authors: Wang Hao , Yang Xiaoxing , Yang Guogang , Zhang Guoling , Li Zheng , Li Lingquan , Huang Naibao
Published: December 2024

Proton exchange membrane fuel cells (PEMFCs) are being pursued for applications in the maritime industry to meet stringent ship emissions regulations. Further basic research is needed to improve the performance of PEMFCs in marine environments. Assembly stress compresses the gas diffusion layer (GDL) beneath the ribs, significantly altering its pore structure and internal transport properties. Accurate evaluation of the PEMFC cathode’s electrochemical performance at the pore scale is critical. This study employs a three-dimensional multicomponent gas transport and electrochemical reaction lattice Boltzmann model to explore the complex interplay between GDL compression and factors such as overpotential, pressure differential, porosity, and porosity gradient on PEMFC performance. The findings indicate that compression accentuates the reduction in oxygen concentration along the flow path and diminishes the minimum current density. Furthermore, compression exacerbates the reduction in current density under varying pressure conditions. Increased local porosity near the catalyst layer (CL) enhances oxygen accessibility and water vapor exclusion, thereby elevating the mean current density. Sensitivity analysis reveals a hierarchy of impact on mean current density, ranked from most to least significant: overpotential, porosity, compression, porosity gradient, and pressure difference. These insights into the multicomponent gas transfer dynamics within compressed GDLs inform strategic structural design enhancements for optimized performance.
Comparison of Single and Multiple Intratracheal Administrations for Pulmonary Toxic Responses of Multi-Walled Carbon Nanotubes in Rats
Authors: Senoh Hideki , Suzuki Masaaki , Kano Hirokazu , Kasai Tatsuya , Fukushima Shoji
Published: December 2024

The purpose of the present study is to contribute to the establishment of a standard method for evaluating the adverse effects of nanomaterials by intratracheal administration. Low and high doses of multi-walled carbon nanotubes (MWCNTs) were administered to rats in a single administration or the same final dose as the single administration but divided over four administrations. Bronchoalveolar lavage examination on day 14 showed an inflammatory reaction and cytotoxicity in the lung, generally greater at the higher dose, and tending to be greater in the rats with four administrations at both the low and high doses. Histopathologic findings showed increased alveolar macrophages and MWCNT deposition (fibers phagocytosed by alveolar macrophages and fibers that were not phagocytosed) in the alveolar space, granulomatous changes, and MWCNT deposition in bronchus-associated lymphoid tissue (BALT) and lung-related lymph nodes on days 14, 28, and 91. In addition, alveolar type II epithelial hyperplasia was observed on day 91, and fibrosis of the alveolar wall was observed on days 28 and 91. Fewer alveolar macrophages with phagocytosed MWCNTs were present at day 91 compared to day 28. MWCNT deposition tended to be higher in the BALT after a single administration, whereas deposition was higher in the lung-related lymph nodes after four administrations. MWCNTs were considered to be transported from the lungs or BALT to the lymph nodes over time. There were no significant differences in MWCNT deposition in the lung after the single administration compared with four administrations at either the low or high doses, and the histopathological findings were similar after single and four administrations, at both the low and high doses. Based on the above findings, a toxicity evaluation of the nanomaterials can be sufficiently performed by intratracheal administration, even with a single intratracheal administration.
Effects of Phytochemicals on Metabolic Diseases and Human Health
Authors: Martín María Ángeles , Ramos Sonia
Published: December 2024

Metabolic diseases constitute a worldwide health concern because of their increasing prevalence and associated mortality [...]
The Long-Term Treatment of Drug-Resistant Migraine with the Modified Atkins Ketogenic Diet: A Single-Center, Retrospective Study
Authors: Francini-Pesenti Francesco , Favaretto Silvia , D’Angelo Matteo , Cacciapuoti Martina , Calò Lorenzo A
Published: December 2024

Despite advances in pharmacological therapies, migraine patients are often drug resistant. Further therapeutic options in this field are, therefore, desirable. Recent studies have highlighted the efficacy of ketogenic diet (KD) on improving migraine, but data on their long-term efficacy and safety are lacking. In this study, we retrospectively evaluated the long-term effectiveness of the modified Atkins ketogenic diet (MAD) in episodic or chronic drug-resistant migraine patients. 52 patients diagnosed with episodic or chronic drug-resistant migraine under modified Atkins ketogenic diet (MAD) were evaluated. In total, 41 patients followed the diet for 6 months and 33 for 12 months. After both 6 and 12 months, frequency, length, and intensity of migraine episodes, as well as the number of medications significantly decreased with respect to the start of the diet. Body mass index, high sensitivity PCR, diastolic blood pressure, fasting plasma insulin and HOMA index were also significantly reduced both after 6 and 12 months. No major metabolic changes were observed during MAD treatment. In conclusion, KD has been shown to be effective and safe in the long-term treatment of drug-resistant migraine. A high dropout rate still remains an important factor, which often limits its use.
The Effect of Carnosine Supplementation on Musculoskeletal Health in Adults with Prediabetes and Type 2 Diabetes: A Secondary Analysis of a Randomized Controlled Trial
Authors: Saadati Saeede , Jansons Paul , Scott David , de Courten Maximilian , Mousa Aya , Feehan Jack , Mesinovic Jakub , de Courten Barbora
Published: December 2024

Background/Objectives: Type 2 diabetes (T2D) is associated with an increased risk of adverse musculoskeletal outcomes likely due to heightened chronic inflammation, oxidative stress, and advanced glycation end-products (AGE). Carnosine has been shown to have anti-inflammatory, anti-oxidative, and anti-AGE properties. However, no clinical trials have examined the impact of carnosine on musculoskeletal health in adults with prediabetes or T2D. Methods: In a randomized, double-blind clinical trial, 49 participants with prediabetes or T2D and without existing musculoskeletal conditions were assigned to receive either 2 g/day carnosine or matching placebo for 14 weeks. Whole-body dual-energy X-ray absorptiometry (DXA) was used to assess body composition, and peripheral quantitative computed tomography (pQCT) was used to assess bone health at the distal and proximal tibia. Results: Forty-three participants completed this study. Carnosine supplementation had no effect on change in hand grip strength (HGS) or upper-limb relative strength (HGS/lean mass) versus placebo. Change in appendicular lean mass, percentage of body fat, visceral fat area, proximal tibial cortical volumetric bone mineral density (vBMD), distal tibial trabecular vBMD, and stress-strain index did not differ with carnosine compared to placebo. Fourteen weeks of carnosine supplementation did not improve muscle strength, body composition, or bone health in adults with prediabetes or T2D. Conclusions: Carnosine supplementation may not be an effective approach for improving musculoskeletal health in adults with prediabetes and T2D without musculoskeletal conditions. However, appropriately powered trials with longer duration are warranted to confirm our findings. The trial was registered at clinicaltrials.gov (NCT02917928).
Protective Effects of Cereal-Based Fermented Beverages Against 5-Fluorouracil-Induced Intestinal Damage in Mice
Authors: Qin Dongze , Fu Wenhui , Sun Yi , Zhao Lingda , Liu Haiwei , Fan Dancai , Tan Dongfei , Ji Xuemeng , Wang Shuo
Published: December 2024

Background: 5-Fluorouracil (5-FU) is a common chemotherapeutic medication used to treat cancer. However, the intestinal tract may sustain oxidative damage as a result. Objectives: The purpose of this study was to clarify the underlying molecular mechanisms and examine the preventive benefits of cereal-based fermented drinks (CFBs) against intestinal injury in mice caused by 5-FU. Methods: The mice were injected intraperitoneally with 5-FU to induce intestinal mucosal and treated with CFB. The factors for intestinal barrier integrity, oxidative stress and inflammation were measured. Results: The findings demonstrated that CFBs had high levels of polyphenol, flavonoids, and peptides and had in vitro high free radical scavenging capacity. Furthermore, CFBs effectively ameliorated 5-FU-induced intestinal epithelium damage, characterized by increasing intestinal tight junctions and reducing apoptosis in intestinal cells. These protective effects may attribute to the increased activity of antioxidant-related enzymes (SOD, CAT, and GSH) as well as decreased amounts of inflammatory and oxidative damage markers (IL-1β, TNF-α, and MDA) in the intestinal tract. Conclusions: Overall, these results show that CFBs can mitigate intestinal damage caused by 5-FU by reducing oxidative stress, suggesting the potential utility of CFBs for therapeutic treatment against intestinal mucositis.
Osteopontin Levels in Maternal Serum, Cord Blood, and Breast Milk According to Gestational Diabetes Mellitus: A Case-Control Study
Authors: Küçükoğlu Keser Merve , Şahin Dilek , Yalçın Sıddika Songül
Published: December 2024

Background/Objectives: The prevalence of gestational diabetes mellitus (GDM) is increasing, and GDM poses health risks for both mothers and newborns. This study investigated the association between GDM and two biomarkers, osteopontin (OPN) and betatrophin (ANGPTL8). Methods: This study involved face-to-face interviews with 165 participants—81 in the GDM group and 84 in the control group—to gather maternal-newborn data. Maternal serum OPN levels, along with cord serum OPN and ANGPTL8 levels, were measured at birth. OPN levels in breast milk were also measured between postnatal days 10–15. Statistical analysis included Student’s t-test for comparing biomarker levels, chi-square tests for GDM incidence across OPN quartile levels, multiple logistic regression for assessing GDM frequency by OPN quartile levels, and the Pearson correlation coefficient to explore relationships between biomarker levels and maternal-newborn characteristics. Results: No significant differences in cord OPN or ANGPTL8 levels were detected between the groups. However, the OPN levels in maternal serum and breast milk were greater in the GDM group than in the control group. We found an association between increasing maternal serum and breast milk OPN levels in quartile groups and the risk of GDM. Additionally, there was a moderate correlation between cord OPN and both maternal serum OPN (r = 0.45) and breast milk OPN (r = 0.43). Conclusions: The differences in OPN levels between the GDM and control groups suggest that OPN may reflect metabolic changes associated with GDM, possibly as a consequence of the condition itself or its treatment. Further research is necessary to validate these findings and uncover the underlying mechanisms involved.
Diet and Microbiota Modulation for Chronic Pouchitis: Evidence, Challenges, and Opportunities
Authors: Puca Pierluigi , Del Gaudio Angelo , Becherucci Guia , Sacchetti Franco , Sofo Luigi , Lopetuso Loris Riccardo , Papa Alfredo , Cammarota Giovanni , Scaldaferri Franco
Published: December 2024

Chronic pouchitis occurs in about 50% of patients undergoing a restorative proctocolectomy for ulcerative colitis. This affection represents a significant therapeutic challenge, particularly for symptomatic patients who do not respond to antibiotic treatments and biologic therapies. Several dietary approaches, including low FODMAP diets and the Mediterranean diet, have shown promising results in improving symptoms and disease burden. The rationale for dietary intervention lies in the reduction in inflammation and modulation of gut microbiota. However, conflicting results and methodological heterogeneity jeopardize the transition of these approaches from the field of research to clinical practice. Together with a nutritional approach, innovative methods of microbiota modulation, including probiotics and fecal microbiota transplantation, are emerging as safe and effective strategies in managing chronic pouchitis. This narrative review analyzes recent advancements in nutritional therapies and microbiota modulation as innovative and complementary approaches for managing chronic pouchitis. After examining microbiota modulation strategies, specifically the effectiveness of probiotics, prebiotics, and fecal microbiota transplantation in restoring microbial diversity and their potential role in alleviating symptoms, the review assesses the available clinical evidence concerning dietary interventions and their impact on gut microbiota. A comprehensive understanding of interventions aimed at modulating the microbiota is crucial for enhancing the effectiveness of conventional therapies. Such strategies may lead to significant improvements in patients’ quality of life and their perception of the disease. However, the variability in microbiota composition, the use of restrictive diets, and the lack of standardized methods for evaluating these interventions remain significant challenges. Future research is essential to improve our understanding of the underlying mechanisms and optimize clinical application.
Genomic Evolution and Phylodynamics of the Species Orthomarburgvirus marburgense (Marburg and Ravn Viruses) to Understand Viral Adaptation and Marburg Virus Disease’s Transmission Dynamics
Authors: Muvunyi Claude Mambo , Mohamed Nouh Saad , Siddig Emmanuel Edwar , Ahmed Ayman
Published: December 2024

In this review, we investigated the genetic diversity and evolutionary dynamics of the Orthomarburgvirus marburgense species that includes both Marburg virus (MARV) and Ravn virus (RAVV). Using sequence data from natural reservoir hosts and human cases reported during outbreaks, we conducted comprehensive analyses to explore the genetic variability, constructing haplotype networks at both the genome and gene levels to elucidate the viral dynamics and evolutionary pathways. Our results revealed distinct evolutionary trajectories for MARV and RAVV, with MARV exhibiting higher adaptability across different ecological regions. MARV showed substantial genetic diversity and evidence of varied evolutionary pressures, suggesting an ability to adapt to diverse environments. In contrast, RAVV demonstrated limited genetic diversity, with no detected recombination events, suggesting evolutionary stability. These differences indicate that, while MARV continues to diversify and adapt across regions, RAVV may be constrained in its evolutionary potential, possibly reflecting differing roles within the viral ecology of the Orthomarburgvirus marburgense species. Our analysis explains the evolutionary mechanisms of these viruses, highlighting that MARV is going through evolutionary adaptation for human-to-human transmission, alarmingly underscoring the global concern about MARV causing the next pandemic. However, further transdisciplinary One Health research is warranted to answer some remaining questions including the host range and genetic susceptibility of domestic and wildlife species as well as the role of the biodiversity network in the disease’s ecological dynamics.