bluuebunny/crossref_metadata_2025 huggingface dataset:
Conditions of free use of works for the purpose of creating educational materials and the distance learning
Authors: Trotska Valentyna
Published: November 2023

The article is devoted to the study of the norms of the Law of Ukraine «On Copyright and Related Rights», which came into force on January 1, 2023 (hereinafter — the Law).It is noted that any learning requires the creation and use of educational material. The works by other authors are often used to create such material. In practice, questions arise as to how to use it without infringing copyright?There are exceptions and limitations to copyright, when it is allowed to freely use works, but subject to the conditions, provided for in the Law.In the article the author analyzes in detail the conditions of free use of works for education. It is established, that it is allowed to:1) reproduction and the making available to the public of works as illustrations in publications, broadcasting programs, sound recordings or video recordings;2) reproduction and the making available to the public of works for distance learning.The article discusses general and special (or additional) conditions. The general conditions of free use of works are investigated, namely:1) indication of the name of the author and the source from which the work was borrowed;2) reproduction of a work if it was lawfully published.Other special (or additional) conditions are also considered.In particular, the scope of use of the work. The problematic issues of using a fragment of a photographic work, a work of fine art as an illustration in an educational publication, given the specifics of such works, are pointed out.The article defines the features of the free use of works as illustrations in publishing by subjects of educational activity from the standpoint of their commercial benefit.It was found that the norms of the Law on the free use for the creation of the educational materials and for distance learning have inconsistencies. It is proved that the relevant conditions provided for by the Law should relate to the free use of works for distance learning, and not to the creation of educational materials. Cases of free use of works for the education are considered in detail, provided that the activity has no independent economic significance or commercial purpose. It is justified that the condition of free use of works, if the actions of a person do not have a commercial purpose, should apply to all cases of free use of works for education. Сonclusions and suggestions are made.
Legal regulation of trade secrets under the legislation of Ukraine and the EU
Authors: Lenho Yuliia
Published: November 2023

Today, the legislation of Ukraine on trade secrets is not systematized and has significant gaps. Relations regarding trade secrets are regulated by the several legal acts relating to various branches of law, and determine only the general principles of legal regulation of such relations. Detection of discrepancies in the legislation of Ukraine and the EU means that the legal protection of trade secrets in Ukraine is uneven and fragmentary, and therefore insufficient. The development of cross-border economic relations between economic entities of Ukraine and the EU requires a unified approach to solving the issue of protecting trade secrets, the absence of which requires studying these issues and developing practical recommendations aimed at approaching the legalregimes of protecting trade secrets in Ukraine and the EU.This article contains the analysis of the provisions of the legislation of Ukraine on the protection of trade secrets, international legal acts in this field and Directive (EU) 2016/943 of the European Parliament and of the Council of 8 June 2016 on the protectionof undisclosed know-how and business information (trade secrets) against their unlawful acquisition, use and disclosure. As a result of the analysis, it was established that: in Ukraine, there is no special legislation on the protection of trade secrets; the concept of trade secret in Ukraine fully corresponds to the concept of a trade secret it the Directive (EU) 2016/943, as well as to the concept of an undisclosed information in the TRIPS Agreement. At the same time, the concept of trade secret in Ukraine needs to be clarified regarding one of the essential signs of trade secret. In addition, in the legislation of Ukraine there is no single approach to the use of the concept of knowhow, as well as regarding the assignment of know-how to IP.

The legislation of Ukraine requires the implementation of the provisions of European legislation on the legal regulation of trade secrets, namely: not classifying trade secrets as IP objects, while it is an addition or an alternative to IP rights; allocation in the «trade secret» of two types of objects - know-how and business information; determination of reengineering by a fair way of identifying trade secrets; determining the conditions for the lawful acquisition, use and disclosure of trade secrets.
Virtual property from the position of the digital intellectual economy: theoretical foundations of digital law
Authors: Butnik-Siversky Oleksandr
Published: November 2023

The article examines the formation of a humandigitalenvironment as a society using digital technologies. It is studied from the standpoint of a real and virtual or material and relatively abstract environment where therights to intellectual property objects dominate as a digital right of a person when using digital tools that enable the use of various digital technologies, as well as fundamental digital rights that require a new understanding. It is noted that the right of virtual property should be distinguished from the right of intellectual property and defined as a specific type of property right. The virtualization of intellectual property is considered as a process of transition from digital intellectual property to digital virtual intellectual property with the corresponding signs of the development or creation of new objects that have an economic value equivalent to material things. In the conditions of digital individualization, the main feature of property rights is the domination of a person over a virtual thing. Virtual space allows the owner of virtual objects to own, use and dispose of them. On the basis of the virtualization of property in the system of using technological platforms, a human-digital system is formed using the latest digital technologies and digital communication with the observance of digital law. Amore complex phenomenon is considered in which virtual owner(s) or implicit owner(s) appear, relying on artificial intelligence. In this regard, the rights are dissolved in a system of transformed computer programs and digital technologies which is gainingpace of development of the digital human rights to digital objects or instruments of virtual property. They are replaced by a virtual or conditional author, a digital administrator as a digital person or a subject of digital legal relations where the value of the real right is lost as the domination of a person over a virtual thing, and only commercial economic dominates an interest that may take the form of a monopoly in the market of digital services. Virtual property is becoming the main legal construct in the system of the digital economy of intellectual property, where there are distinctive features of virtual intellectual property rights in relation to real intellectual property rights.
Prime Mover: Architectural actuation through designed living plant cell-laden scaffolds.
Authors: Robinson Ian , Li Tairan , Pour Ahmad Erhan , Parker Brenda , Cruz Marcos
Published: November 2023

The research project Prime Mover, explores the notion of growing architectural spaces from plant callus tissue. Like buildings, plants are sessile, and bound to the location they are situated in. However, unlike buildings, plants display remarkable plasticity allowing them to respond to environmental stimuli and adapt to stress from their surrounding habitat. By propagating callus tissue extracted from Daucus carota, we explored its ability to differentiate into various functional morphologies through regulating the delivery of various plant hormone concentrations. We developed specialized 3D printing tools for crafting designed callus-laden scaffolds with integrated vascularity, in order to harness the inherent plasticity of plant tissue culture. The hollow interiors of the scaffolds facilitate nutrient circulation for tissue growth and enable precise actuation of plant hormones to guide cell differentiation into intricate forms. The outcomes of this research are presented in a short film, where experiments and prototypes are presented alongside a speculative provocation, envisioning the living material agency and feral qualities of plant tissue. Prime Mover imagines future inhabitable spaces: redefining buildings as dynamic, living entities capable of growing and adapting over time, while nurturing diverse multispecies communities within their organic tissues.
RESILIENSI PADA SISWA-SISWI PRA-REMAJA
Authors: Melani Aprianti  , Dina Syakina  , Heni Nur Fataya  , Utari Rahmawati
Published: November 2023

Resiliensi didefinisikan sebagai suatu proses dinamis dimana seseorang mampu menunjukkan fungsi adaptif dalam menghadapi kesukaran atau kemalangan (adversiti), tidak hanya perubahan secara fisiologis tetapi psikososial harus dilewati pada masa pra remaja. Perubahan tersebut tak ayal menjadi sebuah tantangan yang dihadapi oleh anak pra remaja dan menuntut mereka mengembangkan kemampuan resiliensi. Penelitian ini bertujuan melihat bagaimana kondisi resiliensi pada anak pra remaja dengan metode kuantitatif deskriptif dan menggunakan teknik convenience sampling. Sebanyak 668 responden berusia pra remaja berpartisipasi dalam penelitian ini. Hasil menunjukkan bahwa resiliensi anak pra remaja di Jakarta tergolong sedang (47.3 %) yang artinya cukup baik dalam beradaptasi dan bangkit serta mampu menyelesaikan suatu masalah. Sebanyak 26.7 persen terkategori rendah dan sebanyak 25.7 persen terkategori tinggi, dimana perempuan menunjukkan kemampuan resiliensi lebih tinggi dibandingkan laki-laki. Tingkat vulnerabiliti juga didominasi oleh kategori sedang (45.7 %) yang mengindikasikan bahwa anak pra remaja sudah cukup mampu mengolah reaksi emosi negatif dalam dirinya. Implikasi penelitian menunjukkan bahwa masa pra remaja penting untuk memiliki kemampuan resilien, oleh karena itu saran dalam penelitian ini adalah baik sekolah dan orang tua harus terlibat dan berperan signifikan untuk dapat meningkatkan kemampuan resiliensi anak pra remaja.
Core binding factor subunit β plays diverse and essential roles in the male germline
Authors: Rahmawati Mustika , Stadler Kassie M. , Lopez-Biladeau Blanca , Hoisington Tia M. , Law Nathan C.
Published: November 2023

Much of the foundation for lifelong spermatogenesis is established prior to puberty, and disruptions during this developmental window negatively impact fertility long into adulthood. However, the factors that coordinate prepubertal germline development are incompletely understood. Here, we report that core-binding factor subunit-β (CBFβ) plays critical roles in prepubertal development and the onset of spermatogenesis. Using a mouse conditional knockout (cKO) approach, inactivation of Cbfb in the male germline resulted in rapid degeneration of the germline during the onset of spermatogenesis, impaired overall sperm production, and adult infertility. Utilizing a different Cre driver to generate another Cbfb cKO model, we determined that the function of CBFβ in the male germline is likely limited to undifferentiated spermatogonia despite expression in other germ cell types. Within undifferentiated spermatogonia, CBFβ regulates proliferation, survival, and overall maintenance of the undifferentiated spermatogonia population. Paradoxically, we discovered that CBFβ also distally regulates meiotic progression and spermatid formation but only with Cbfb cKO within undifferentiated spermatogonia. Spatial transcriptomics revealed that CBFβ modulates cell cycle checkpoint control genes associated with both proliferation and meiosis. Taken together, our findings demonstrate that core programs established within the prepubertal undifferentiated spermatogonia population are necessary for both germline maintenance and sperm production.
Case Report: Successful surgical management of a challenging primary cardiac angiosarcoma
Authors: Li Yichen , Ai Yinze , Tang Weijie , Liu Jijia , Yang Jinfu , Fan Chengming
Published: November 2023

Primary cardiac tumors are exceptionally rare, with malignant tumor occurrences ranging from 0.0017% to 0.28%. Among these, primary cardiac angiosarcoma (PCA) stands as the most prevalent malignancy, primarily impacting the right cardiac system. In this case report, we present the instance of a 44-year-old woman who recently exhibited acute chest discomfort and was subsequently diagnosed with a microangiosarcoma within the right atrium and superior vena cava. Diagnostic modalities including chest x-rays, CT, MRI, and PET-CT were instrumental in pinpointing the tumor's location and nature. Surgical excision followed by pathological and immunological examinations confirmed the diagnosis. The patient's recovery post-surgery has been encouraging, with successful follow-up chemoradiotherapy administered. Despite advancements, devising optimal strategies for enhancing patient survival and quality of life in angiosarcoma cases remains a pressing research challenge.
“Aggravated, everyday aggravated—everyday”: senseMaking to truth tell, talkback and find belonging in educational research
Authors: Clark Emily B.
Published: November 2023

This article explores an analytical, reflexive, epistemological, and liberatory research approach I call senseMaking. This article draws upon data from a larger narrative study of participants who work(ed) and/or learn(ed) in self-contained special educational environments in New York City. I use creative writing and quilting as examples of senseMaking tools to complicate and question the deficit-laden narratives and research that currently inform decision making about special education and traditional methods of educational research.
Survival benefit of local consolidative therapy for patients with single-organ metastatic pancreatic cancer: a propensity score-matched cross-sectional study based on 17 registries
Authors: Hu Xiaolong , Hu Dan , Fu Bowen , Li Hongqi , Ren Gang , Liu Hefei , Song Jiazhao , Kang Xiaoli , Wang Xuan , Pang Haifeng , Liu Chen , Zhang Jianchun , Wang Yingjie
Published: November 2023

Background

The continuous exploration of oligometastatic disease has led to the remarkable achievements of local consolidative therapy (LCT) and favorable outcomes for this disease. Thus, this study investigated the potential benefits of LCT in patients with single-organ metastatic pancreatic ductal adenocarcinoma (PDAC).

Methods

Patients with single-organ metastatic PDAC diagnosed between 2010 - 2019 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. Propensity score matching (PSM) was performed to minimize selection bias. Factors affecting survival were assessed by Cox regression analysis and Kaplan-Meier estimates.

Results

A total of 12900 patients were identified from the database, including 635 patients who received chemotherapy combined with LCT with a 1:1 PSM with patients who received only chemotherapy. Patients with single-organ metastatic PDAC who received chemotherapy in combination with LCT demonstrated extended median overall survival (OS) by approximately 57%, more than those who underwent chemotherapy alone (11 vs. 7 months, p < 0.001). Furthermore, the multivariate Cox regression analysis revealed that patients that received LCT, younger age (< 65 years), smaller tumor size (< 50 mm), and lung metastasis (reference: liver) were favorable prognostic factors for patients with single-organ metastatic PDAC.

Conclusion

The OS of patients with single-organ metastatic pancreatic cancer who received LCT may be prolonged compared to those who received only chemotherapy. Nevertheless, additional prospective randomized clinical trials are required to support these findings.
Feasibility of parathyroid gland autofluorescence imaging after indocyanine green fluorescence angiography
Authors: Richard Marieke , Rizo Philippe
Published: November 2023

Background

In thyroid surgery, autofluorescence allows the parathyroid glands (PTGs) to be located very early to protect them. Moreover, indocyanine green (ICG) fluorescence angiography (ICG-FA) allows for assessing the viability of the PTGs and identifying blood vessels to preserve them. The main limitation of using ICG-FA is that once ICG has been injected, it is no longer possible to observe PTG autofluorescence using existing devices. This study aimed to describe an approach that allows for visualization of the PTGs by autofluorescence, even after ICG injection.

Methods

We redesigned the FLUOBEAM® LX system to excite fluorescence at 685 nm and detect fluorescence between 700 and 900 nm. This device had short-pass filters at 775 nm that helped to split the contributions of the PTG autofluorescence and ICG fluorescence. Tests were performed on extemporaneous PTG preparations placed next to ICG droplets to assess for rejection of the ICG signal.

Results

A low-pass filter at 775 nm detected 60% of the autofluorescence signals and 10% of the ICG signals.

Conclusion

These findings support the possibility of visualizing PTG autofluorescence despite multiple ICG injections and measuring the balance between ICG and autofluorescence signals.
The effects of gonadotropin-releasing hormone agonist on final adult height among girls with early and fast puberty
Authors: Tseng Chin-Hui , Lee Yann-Jinn , Huang Chi-Yu , Wu Yi-Lei , Wang Lu-Ting , Lin Chao-Hsu , Cheng Bi-Wen , Lo Fu-Sung , Chang Yu-Jun , Ting Wei-Hsin
Published: November 2023

Introduction

This study aimed to explore the impact of gonadotropin-releasing hormone agonists (GnRHa) on final adult height (FAH) in girls with early and fast puberty.

Methods

A retrospective study was conducted by reviewing data from the medical records of the Pediatric Endocrinology Clinics between January 1, 2010, and December 31, 2020, at MacKay Children’s Hospital. The treatment group included 109 patients who received 3.75 mg monthly for at least 1 year, whereas the control group consisted of 95 girls who received no treatment.

Results

The treatment group was significantly older at the time of inclusion(chronological age (CA1), treatment vs. control, 8.7 vs. 8.4 years, p < 0.001), had a more advanced bone age (BA) (BA1, 11.5 vs. 10.8 years, p < 0.001), BA1-CA1 (2.7 vs. 2.2 years, p < 0.001), and shorter predicted adult height (PAH1) (153.3 vs. 157.1 cm, p = 0.005) that was significantly lower than their target height (Tht)(PAH1-Tht, −3.9 vs. −1.3 cm, p = 0.039). The FAHs of the GnRHa and the control group were similar (157.0 vs. 156.7 cm, p = 0.357) and were not significantly different from their Tht (FAH vs. Tht in the GnRHa group, 157.0 vs. 157.0 cm; control group, 156.7 vs. 157.0 cm). In the subgroup analysis, FAH was significantly higher after GnRHa treatment in those with PAH1 less than 153 cm and Tht (154.0 vs. 152.0 cm, p = 0.041), and those whose CA1 was between 8 and 9 years (158.0 vs. 155.4 cm, p = 0.004). We defined satisfactory FAH outcome as FAH-PAH1≥5 cm and significant factors were GnRHa therapy, PAH1 shorter than their Tht, age younger than 9 years, and faster growth velocity during the first year.

Discussion

GnRHa is effective in restoring the Tht in some early and fast pubertal girls, especially in those with poorly PAH (PAH lower than 153 cm and shorter than their target height). A younger age at initiation of treatment and a faster growth velocity during treatment are associated with a better height gain.
Multiple-layer energy management strategy for charging station optimal operation considering peak and valley shaving
Authors: Qian Bin , Song Min , Ke Song , Zhang Fan , Luo Bin , Wang Ji , Tang Jianlin , Yang Jun
Published: November 2023

Existing vehicle-to-grid (V2G) applications are aimed at the power grid and the government. It is difficult for charging stations (CSs) to execute the schedules in real time. To figure out the multiple-layer energy management from the perspective of CS, the dispatch potential assessment model is constructed based on the EV users’ charging demand and Minkowski summation. And the optimal energy management schedule model of CS with ESS is proposed considering peak shaving and valley filling under the time-in-use tariff. Besides, the real-time charging control model of EVs in CS is designed under the premise of meeting the charging needs. The simulation results show that the proposed strategy can promote CS operation revenues and track the scheduling plan of CS. The arbitrage of tariffs and peak shaving ancillary services are realized while the charging loads of CSs are smoothed by the charging/discharging of ESS. The proposed strategy is applicable for the CS aggregators and can help the grid operators for dispatch schedules.
How can high-quality development improve the ecotourism efficiency in the region of ecological constraints of China? Empirical evidence from the Yellow River Basin
Authors: Hao Junqing , Guo Ying , Wu Mengyao , Luo Zhixin
Published: November 2023

Despite the massive impacts of ecotourism on regional development, only limited papers empirically examined the responses of the regional development factors to ecotourism in the context of an ecological constraints region. To fill this gap, the primary aim of this paper is to reveal how ecotourism efficiency is affected by quality regional development in the region of ecological constraints. The second aim is to investigate the moderating role of ecological constraints in building relationships of ecotourism efficiency and quality regional development factors. The research was conducted in the Yellow River Basin, a prime area for ecological protection and high-quality regional development in China. Data gathered from 2010 to 2019 were used to analyze ecotourism efficiency by using the super–slacks-based measurement method. Findings indicated that four quality regional development factors—innovative, green, open, and shared factors—have positive impacts on ecotourism efficiency in the Yellow River Basin. Ecological constraints moderate the relationship between ecotourism efficiency and quality regional development. The study makes a significant contribution to the literature in terms of both managing the ecological constraints and improving the sustainability of ecotourism in the region of quality development.
Effects of sodium butyrate on growth performance, antioxidant status, inflammatory response and resistance to hypoxic stress in juvenile largemouth bass (Micropterus salmoides)
Authors: Hou Dongqiang , Li Min , Li Peijia , Chen Bing , Huang Wen , Guo Hui , Cao Junming , Zhao Hongxia
Published: November 2023

The aim of this study was to investigate the effects of sodium butyrate (SB) supplementation on growth performance, antioxidant enzyme activities, inflammatory factors, and hypoxic stress in largemouth bass (Micropterus salmoides). Diets were supplemented with different doses of SB at 0 (SB0), 0.5 (SB1), 1.0 (SB2) and 2.0 (SB3) g/kg. The hypoxic stress experiment was performed after 56 days of culture. The results showed that compared with the SB0 group, the final body weight, weight gain rate and protein deposition rate of the SB3 group were significantly increased (P<0.05), while FCR was significantly decreased (P<0.05). The contents of dry matter, crude lipids, and ash in the SB2 group were significantly higher than those in the SB0 group (P<0.05). The urea level was significantly decreased (P<0.05), and the glucose content was significantly increased (P<0.05) in the SB supplement group. Compared with the SB0 group, the SB2 group had significant reductions in the levels of serum triglyceride, cholesterol, elevated-density lipoprotein cholesterol, and low-density lipoprotein (P<0.05), and significant reductions in the levels of liver alkaline phosphatase and malondialdehyde (P<0.05). The total antioxidant capacity of the SB1 group was higher than that of other groups (P<0.05). Compared with the SB0 group, the mRNA expression of TLR22, MyD88, TGF-β1, IL-1β and IL-8 in the SB2 group significantly decreased (P<0.05). The cumulative mortality rate was significantly decreased in the SB2 and SB3 groups in comparison with that in the SB0 group after three hours of hypoxic stress (P<0.05). In a 56-day feeding trial, SB enhanced largemouth bass growth by increasing antioxidant enzyme activity and inhibiting TLR22-MyD88 signaling, therefore increasing cumulative mortality from hypoxic stress in largemouth bass.
Side effects of COVID-19 vaccines in the middle eastern population
Authors: Murished Ghida M. , Dandachi Iman , Aljabr Waleed
Published: November 2023

The COVID-19 pandemic has caused severe worldwide health concerns since its first description as the SARS-COV-2 virus in December 2019. The wide dissemination of this virus, together with the lack of treatment, prompted vaccine development within a short period of time to elicit a protective immunity against COVID-19. Due to their rapid development, potential subsequent side effects of COVID-19 vaccines were overlooked, which might lead to many health concerns. This is especially true for patients at a greater risk of harm from COVID-19, such as pregnant women, children, and patients with pre-existing chronic diseases. In this review, we provide a summary of common to rare side effects of administrated COVID-19 vaccines in a Middle Eastern population. We have found that the distinction between side effects from COVID-19 vaccines in terms of frequency and severity is attributed to the differences in study populations, gender, and age. Pain at the injection site, fever, headache, fatigue, and muscle pain were the most common reported side effects. Vaccinated subjects with previous COVID-19 infection exhibited an equivalent neutralizing response after just one dose compared to two doses of vaccine. Consequently, individuals who experienced more side effects had significantly higher antibody levels. This indicates that having better immunity correlates with higher antibody levels, leading to a higher frequency of vaccine side effects. Individuals with underlying comorbidities, particularly having known allergies and with illnesses such as diabetes and cancer, might be more prone to post-vaccination side effects. Studies of a high-risk population in Middle Eastern countries are limited. Future studies should be considered to determine long-term side effects, side effects after booster doses, and side effect differences in cases of heterologous and homologous vaccination for better understanding and proper handling of high-risk populations and patients who experience these side effects.
Serum, spleen metabolomics and gut microbiota reveals effect of catalpol on blood deficiency syndrome caused by cyclophosphamide and acetylphenylhydrazine
Authors: Zhang Wensen , Cui Na , Su Fazhi , Sun Yanping , Li Biao , Ren Yupeng , Wang Ping , Bai Haodong , Guan Wei , Yang Bingyou , Wang Qiuhong , Kuang Haixue
Published: November 2023

Catalpol (CA), extracted from Rehmannia Radix, holds extensive promise as a natural medicinal compound. This study employed 16S rRNA gene sequencing and combined serum and spleen metabolomics to profoundly investigate the therapeutic effects of CA on blood deficiency syndrome (BDS) and the underlying mechanisms. Notably, CA exhibited effectiveness against BDS induced by cyclophosphamide (CP) and acetylphenylhydrazine (APH) in rats-CA substantially elevated levels of crucial indicators such as erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-a), and interleukin-6 (IL-6). Additionally, CA could alleviate peripheral blood cytopenia. Furthermore, the analysis of 16S rRNA revealed that CA had the potential to reverse the Firmicutes/Bacteroidetes (F/B) ratio associated with BDS. Through comprehensive serum and spleen metabolomic profiling, we successfully identified 22 significant biomarkers in the serum and 23 in the spleen, respectively. Enrichment analysis underscored Glycerophospholipid metabolism and Sphingolipid metabolism as potential pathways through which CA exerts its therapeutic effects on BDS.
Construction and experimental validation of a signature for predicting prognosis and immune infiltration analysis of glioma based on disulfidptosis-related lncRNAs
Authors: Guo Youwei , Jiang Zhipeng , Chen Quan , Xie Dongcheng , Zhou Yi , Yin Wen , Wang Zihan , Wang Binbin , Ren Caiping , Jiang Xingjun
Published: November 2023

Backgrounds

Disulfidptosis, a newly discovered mechanism of programmed cell death, is believed to have a unique role in elucidating cancer progression and guiding cancer therapy strategies. However, no studies have yet explored this mechanism in glioma.

Methods

We downloaded data on glioma patients from online databases to address this gap. Subsequently, we identified disulfidptosis-related genes from published literature and verified the associated lncRNAs.

Results

Through univariate, multivariate, and least absolute shrinkage and selection operator (LASSO) regression algorithms analyses, we identified 10 lncRNAs. These were then utilized to construct prognostic prediction models, culminating in a risk-scoring signature. Reliability and validity tests demonstrated that the model effectively discerns glioma patients’ prognosis outcomes. We also analyzed the relationship between the risk score and immune characteristics, and identified several drugs that may be effective for high-risk patients. In vitro experiments revealed that LINC02525 could enhances glioma cells’ migration and invasion capacities. Additionally, knocking down LINC02525 was observed to promote glioma cell disulfidptosis.

Conclusion

This study delves into disulfidptosis-related lncRNAs in glioma, offering novel insights into glioma therapeutic strategies.
Environmental impacts of shallow water mining in the Baltic Sea
Authors: Dargahi Bijan
Published: November 2023

The discovery of rare metal resources in international waters has raised seabed mining claims for large areas of the bottom. There is abundant scientific evidence of major negative consequences for the maritime environment, such as the destruction of natural landforms and the fauna that depend on them, as well as the production of enormous silt plumes that disrupt aquatic life. This study investigated the environmental risks of seabed mining for metal resources in the Baltic Sea using a combination of hydrodynamic, particle-tracking, and sediment-transport models. The models were applied for ten years i.e., 2000-2009 under prevailing conditions to simulate seabed mining operations. The focus was on sediment concentration near the seabed and its spread. The mean background concentrations were low with small seasonal bed-level variations throughout the Baltic Sea Basin. Late summer and early autumn periods were the most active. Seabed mining significantly alters the dynamics of sediment suspensions and bed level variations. The concentrations increase unsustainably to high levels, posing a serious threat to the ecological health of the Baltic Sea. The Gotland basins in the Baltic Sea are the most susceptible to mining. The bed level variations will be ten-fold, exposing the highly contaminated sediments at the seabed to the flow. In less than a year, 30-60% of the total particles released in each basin reached the thermocline layers. This study suggests that seabed mining in the Baltic Sea is not sustainable.
Porcine epidemic diarrhea virus causes diarrhea by activating EGFR to regulates NHE3 activity and mobility on plasma membrane
Authors: Zhang YiLing , Zhang Shujuan , Sun Zhiwei , Liu Xiangyang , Liao Guisong , Niu Zheng , Kan ZiFei , Xu ShaSha , Zhang JingYi , Zou Hong , Zhang Xingcui , Song ZhenHui
Published: November 2023

As part of the genus Enteropathogenic Coronaviruses, Porcine Epidemic Diarrhea Virus (PEDV) is an important cause of early diarrhea and death in piglets, and one of the most difficult swine diseases to prevent and control in the pig industry. Previously, we found that PEDV can block Na+ absorption and induce diarrhea in piglets by inhibiting the activity of the sodium-hydrogen ion transporter NHE3 in pig intestinal epithelial cells, but the mechanism needs to be further explored. The epidermal growth factor receptor (EGFR) has been proved to be one of the co-receptors involved in many viral infections and a key protein involved in the regulation of NHE3 activity in response to various pathological stimuli. Based on this, our study used porcine intestinal epithelial cells (IPEC-J2) as an infection model to investigate the role of EGFR in regulating NHE3 activity after PEDV infection. The results showed that EGFR mediated viral invasion by interacting with PEDV S1, and activated EGFR regulated the downstream EGFR/ERK signaling pathway, resulting in decreased expression of NHE3 and reduced NHE3 mobility at the plasma membrane, which ultimately led to decreased NHE3 activity. The low level of NHE3 expression in intestinal epithelial cells may be a key factor leading to PEDV-induced diarrhea in newborn piglets. This study reveals the importance of EGFR in the regulation of NHE3 activity by PEDV and provides new targets and clues for the prevention and treatment of PEDV-induced diarrhea in piglets.
Emerging trends and focus of research on the relationship between traumatic brain injury and gut microbiota: a visualized study
Authors: Du Qiujing , Li Qijie , Liao Guangneng , Li Jiafei , Ye Peiling , Zhang Qi , Gong Xiaotong , Yang Jiaju , Li Ka
Published: November 2023

Background

Traumatic brain injury (TBI) is one of the most serious types of trauma and imposes a heavy social and economic burden on healthcare systems worldwide. The development of emerging biotechnologies is uncovering the relationship between TBI and gut flora, and gut flora as a potential intervention target is of increasing interest to researchers. Nevertheless, there is a paucity of research employing bibliometric methodologies to scrutinize the interrelation between these two. Therefore, this study visualized the relationship between TBI and gut flora based on bibliometric methods to reveal research trends and hotspots in the field. The ultimate objective is to catalyze progress in the preclinical and clinical evolution of strategies for treating and managing TBI.

Methods

Terms related to TBI and gut microbiota were combined to search the Scopus database for relevant documents from inception to February 2023. Visual analysis was performed using CiteSpace and VOSviewer.

Results

From September 1972 to February 2023, 2,957 documents published from 98 countries or regions were analyzed. The number of published studies on the relationship between TBI and gut flora has risen exponentially, with the United States, China, and the United Kingdom being representative of countries publishing in related fields. Research has formed strong collaborations around highly productive authors, but there is a relative lack of international cooperation. Research in this area is mainly published in high-impact journals in the field of neurology. The “intestinal microbiota and its metabolites,” “interventions,” “mechanism of action” and “other diseases associated with traumatic brain injury” are the most promising and valuable research sites. Targeting the gut flora to elucidate the mechanisms for the development of the course of TBI and to develop precisely targeted interventions and clinical management of TBI comorbidities are of great significant research direction and of interest to researchers.

Conclusion

The findings suggest that close attention should be paid to the relationship between gut microbiota and TBI, especially the interaction, potential mechanisms, development of emerging interventions, and treatment of TBI comorbidities. Further investigation is needed to understand the causal relationship between gut flora and TBI and its specific mechanisms, especially the “brain-gut microbial axis.”
Age-related differences in risk factors, clinical characteristics, and outcomes for intracerebral hemorrhage
Authors: Chen Chu , Xie Yanfang , Pu Mingjun , Deng Lan , Li Zuoqiao , Yang Tiannan , Yin Hao , Zhang Zhehao , Lv Xinni , Liu Xueyun , Cheng Jing , Li Qi
Published: November 2023

Background and purpose

Intracerebral hemorrhage (ICH) is a severe form of stroke that remains understudied in the young adults. We aimed to investigate the clinical presentation, and risk factors associated with ICH in this age group and compare them to older patients.

Methods

Our study included ICH patients admitted between March 2016 and December 2021 in the First Affiliated Hospital of Chongqing Medical University from our ongoing prospective cohort database. Demographic characteristics, etiology, risk factors, and clinical outcomes were compared between elderly and young patients. Furthermore, logistic regression analysis was employed to explore risk factors associated with the functional outcome at 3-months.

Results

We selected 1,003 patients (mean age, 59.9 ±13.8 years old), 746 (74.4%) patients were aged >50 years. The logistic regression analysis showed young patients have a higher proportion of secondary ICH, higher white blood cell count and higher body mass index (BMI), but less diabetes mellitus. Of all patients, predictors of 3-month functional independence was first-ever ICH and age ≤50 years. The history of nephropathy and stroke, higher baseline NIHSS score, larger hematoma volume, and the presence of hydrocephalus were associated with poor outcomes. And the white blood cell count could significantly influence the prognosis among young ICH patients. Three-month functional outcome based on modified Rankin scale score was better in young patients than the elderly (OR, 1.232; 95% CI, 1.095–1.388; p < 0.001).

Conclusions

The highest incidence of ICH occurs in the age groups of 50–59 and 60–69. ICH in young adults had higher white blood cell and BMI compared to the elderly, and differs in etiological distribution. The young patients also had similar short-term mortality but more favorable functional outcomes than the elderly. Furthermore, NIHSS score and larger hematoma volumes were associated with poor outcome in all patients.
A survey of path planning of industrial robots based on rapidly exploring random trees
Authors: Luo Sha , Zhang Mingyue , Zhuang Yongbo , Ma Cheng , Li Qingdang
Published: November 2023

Path planning is an essential part of robot intelligence. In this paper, we summarize the characteristics of path planning of industrial robots. And owing to the probabilistic completeness, we review the rapidly-exploring random tree (RRT) algorithm which is widely used in the path planning of industrial robots. Aiming at the shortcomings of the RRT algorithm, this paper investigates the RRT algorithm for path planning of industrial robots in order to improve its intelligence. Finally, the future development direction of the RRT algorithm for path planning of industrial robots is proposed. The study results have particularly guided significance for the development of the path planning of industrial robots and the applicability and practicability of the RRT algorithm.
The first synapse in vision in the aging mouse retina
Authors: Gierke Kaspar , Lux Uwe Thorsten , Regus-Leidig Hanna , Brandstätter Johann Helmut
Published: November 2023

Vision is our primary sense, and maintaining it throughout our lifespan is crucial for our well-being. However, the retina, which initiates vision, suffers from an age-related, irreversible functional decline. What causes this functional decline, and how it might be treated, is still unclear. Synapses are the functional hub for signal transmission between neurons, and studies have shown that aging is widely associated with synaptic dysfunction. In this study, we examined the first synapse of the visual system – the rod and cone photoreceptor ribbon synapse – in the mouse retina using light and electron microscopy at 2–3 months, ~1 year, and >2 years of age. We asked, whether age-related changes in key synaptic components might be a driver of synaptic dysfunction and ultimately age-related functional decline during normal aging. We found sprouting of horizontal and bipolar cells, formation of ectopic photoreceptor ribbon synapses, and a decrease in the number of rod photoreceptors and photoreceptor ribbon synapses in the aged retina. However, the majority of the photoreceptors did not show obvious changes in the structural components and protein composition of their ribbon synapses. Noteworthy is the increase in mitochondrial size in rod photoreceptor terminals in the aged retina.
IgA-dominant postinfectious glomerulonephritis: a case report
Authors: Moreno-Alvarado Rodolfo , Navarro-Blackaller Guillermo , De Leon-Pérez Werner , Armas-Eguizabal David , Chávez-Iñiguez Jonathan
Published: November 2023

Introduction

Acute postinfectious glomerulonephritis (APIGN) is an immunological glomerular disease that is an important health issue in developing countries. The incidence remains high in developing countries with a male-to-female ratio of 2:1 and age predominantly above 50 years. In this case study, we present a patient with a history of Staphylococcus epidermidis infection, a past medical history of diabetes mellitus, and histopathological findings of APIGN with Immunoglobulin A (IgA) deposition.

Methods

A 58-year-old male presented to the emergency room with a 6-day history of severe low back pain. Three days later, the patient developed fever, chills, abdominal pain in the upper quadrant and a subsequent lower limb cellulitis. Various immunological tests, imaging studies, and kidney biopsy were performed to arrive at a diagnosis.

Results

Following the diagnosis and treatment of Cholangitis and Staphylococcus epidermidis, further investigation led to a diagnosis of IgA-dominant APIGN. IgA-dominant APIGN was treated with antibiotics, renin-angiotensin-aldosterone system inhibitors and steroids, and the patient was discharged from the hospital.

Conclusion

In developing countries, APIGN is a relatively common presentation of kidney damage due to acute kidney injury and nephritic syndrome. IgA-dominant APIGN is a rare but increasingly recognized morphological variant in which IgA is the sole or dominant immunoglobulin. This unique presentation and multidisciplinary approach for diagnosing and treating IgA-dominant APIGN need to be considered and understood by healthcare professionals to better help these patients. Further investigation is needed to understand the best treatment of this IgA-dominant APIGN presentation and its prognosis.
Retreatment with a pipeline embolization device for recanalized aneurysms following stent-assisted coiling embolization
Authors: Ling Yuhui , Liu Jie , Zhou Liyun , Xiang Xiuzhi , Wang Peiming
Published: November 2023

Background and purpose

Flow diverters have emerged as viable alternatives for the retreatment of recanalized aneurysms following stent-assisted coiling embolization. In this study, we aim to present our experience of retreatment for such aneurysms using the pipeline embolization device (PED).

Materials and methods

This case series presents a retrospective single-center analysis of patients with recanalized aneurysms who underwent retreatment using the PED between July 2019 and April 2023, subsequent to stent-assisted coiling embolization.

Results

The study includes five female patients, whose relevant clinical data were recorded. All patients had aneurysms located in the internal carotid artery, comprising two blood blister-like aneurysms and two giant aneurysms. Prior to the retreatment, two LVIS stents, two enterprise stents, and one solitaire stent were implanted. Among the five patients, one experienced a fatal post-operative subarachnoid hemorrhage, while two patients achieved complete embolization, and another patient achieved near-complete embolization during the last follow-up. Furthermore, one patient faced challenges during the placement of the PED and was unable to achieve successful deployment. We propose four overlapping relationships between a newly implanted PED and a previously deployed stent: (1) PED covering only the proximal end of the previous stent, (2) PED covering only the distal end of the previous stent, (3) PED covering both the proximal and distal ends of the previous stent, and (4) PED deployed within the previous stent. Antiplatelet therapy at our center involved daily dual therapy with aspirin (100 mg/day) and clopidogrel (75 mg/day) for at least 5 days before PED placement. Intra-arterial bolus administration of tirofiban (5 mcg/kg) was administered during or immediately after PED deployment, followed by a maintenance dose of 0.08 mcg/kg/min IV infusion for at least 24–48 h if necessary. Postprocedural dual antiplatelet therapy included clopidogrel (75 mg/day) for 6 months and aspirin (100 mg/day) for 12 months.

Conclusion

The findings of this study support the efficacy of the PED for the retreatment of recanalized aneurysms following stent-assisted coiling embolization.
Efficacy of repetitive transcranial magnetic stimulation at different sites for peripheral facial paralysis: a prospective cohort study
Authors: Liu Zicai , Wen Xin , Shao Yuchun , Wan Zihao , Liu Bangliang , Wang Risheng , Liu Huiyu
Published: November 2023

Background

There are very few studies on transcranial magnetic stimulation (TMS) therapy for facial paralysis and no studies comparing the efficacy of central and peripheral TMS in the treatment of peripheral facial paralysis (PFP).

Purpose

To observe the therapeutic effect and security of central and peripheral repetitive transcranial magnetic stimulation (rTMS) on PFP.

Methods

Patients with unilateral onset of peripheral facial paralysis within 1 month were prospectively recruited, 97 patients with PFP were divided into the peripheral group, central group, and control group. The control group was given common treatment (drug therapy and acupuncture), and the peripheral and central groups received rTMS in addition to conventional treatment. After 2 weeks of treatment, the House-Brackmann (HB) grading scale, Sunnybrook facial grading system (SFGS), and modified Portmann scale (MPS) were used to evaluate the facial muscle function of patients in the three groups.

Result

After 2 weeks of rTMS treatment, the HBGS/SFGS/MPS scores of the three groups were significantly better than before (p < 0.05), and the mean change values of HBGS, SFGS, and MPS scores were significantly higher in participants in Peripheral Group (p < 0.001; p < 0.001; p = 0.003; respectively) and Central Group (p = 0.004; p = 0.003; p = 0.009; respectively) than in Control Group. But the mean change values of HBGS, SFGS, and MPS scores showed no significant differences in participants in the Peripheral Group than in the Central Group (p = 0.254; p = 0.139; p = 0.736; respectively) after 2 weeks of treatment (p > 0.05).

Conclusion

Our study shows that rTMS can be a safe and effective adjuvant therapy for patients with PFP. Preliminary studies have shown that both peripheral and central stimulation can effectively improve facial nerve function, but there is no significant difference in the efficacy of the two sites.