bluuebunny/crossref_metadata_2025 huggingface dataset:
Probucol Pharmacological and Bio-Nanotechnological Effects on Surgically Transplanted Graft Due to Powerful Anti-Inflammatory, Anti-Fibrotic and Potential Bile Acid Modulatory Actions
Authors: Mooranian Armin , Ionescu Corina Mihaela , Wagle Susbin Raj , Kovacevic Bozica , Walker Daniel , Jones Melissa , Chester Jacqueline , Foster Thomas , Johnston Edan , Mikov Momir , Atlas Marcus D. , Al-Salami Hani
Published: August 2021

Introduction. A major obstacle in islet transplantation and graft survival pre and post transplantation is islet apoptosis due to mainly inflammatory bio molecules released during islet harvesting and post graft transplantation and hence, subsequent graft fibrosis and failure. This study aimed to investigate if incorporation of the anti-inflammatory anti-hyperlipidaemic drug probucol (PB) would improve islet-graft survival and function, post transplantation in Type 1 diabetes (T1D). Methods. T1D was induced in mice, and biological profiles of the diabetic mice transplanted PB-microencapsulated islets harvested from healthy syngeneic mice were measured. Results and Conclusion. Compared with sham (no PB), the treated group showed significant reduction in serum levels of interleukin-1β, interleukin-6, interleukin-12, interleukin-17, and tumour necrosis factor-α, accompanied by a 3-fold increase in survival duration, which suggests PB islet-protective effects, post transplantation.
Effect of Selected Antidepressants on Placental Homeostasis of Serotonin: Maternal and Fetal Perspectives
Authors: Horackova Hana , Karahoda Rona , Cerveny Lukas , Vachalova Veronika , Ebner Ronja , Abad Cilia , Staud Frantisek
Published: August 2021

Depression is a prevalent condition affecting up to 20% of pregnant women. Hence, more than 10% are prescribed antidepressant drugs, mainly serotonin reuptake inhibitors (SSRIs) and selective serotonin and noradrenaline reuptake inhibitors (SNRIs). We hypothesize that antidepressants disturb serotonin homeostasis in the fetoplacental unit by inhibiting serotonin transporter (SERT) and organic cation transporter 3 (OCT3) in the maternal- and fetal-facing placental membranes, respectively. Paroxetine, citalopram, fluoxetine, fluvoxamine, sertraline, and venlafaxine were tested in situ (rat term placenta perfusion) and ex vivo (uptake studies in membrane vesicles isolated from healthy human term placenta). All tested antidepressants significantly inhibited SERT- and OCT3-mediated serotonin uptake in a dose-dependent manner. Calculated half-maximal inhibitory concentrations (IC50) were in the range of therapeutic plasma concentrations. Using in vitro and in situ models, we further showed that the placental efflux transporters did not compromise mother-to-fetus transport of antidepressants. Collectively, we suggest that antidepressants have the potential to affect serotonin levels in the placenta or fetus when administered at therapeutic doses. Interestingly, the effect of antidepressants on serotonin homeostasis in rat placenta was sex dependent. As accurate fetal programming requires optimal serotonin levels in the fetoplacental unit throughout gestation, inhibition of SERT-/OCT3-mediated serotonin uptake may help explain the poor outcomes of antidepressant use in pregnancy.
Nanostructured Lipid Carriers (NLCs) for Oral Peptide Drug Delivery: About the Impact of Surface Decoration
Authors: Shahzadi Iram , Fürst Andrea , Knoll Patrick , Bernkop-Schnürch Andreas
Published: August 2021

This study was aimed to evaluate the impact of surfactants used for nanostructured lipid carriers (NLCs) to provide enzymatic protection for incorporated peptides. Insulin as a model peptide was ion paired with sodium dodecyl sulfate to improve its lipophilicity. Three NLC formulations containing polyethylene glycol ester (PEG-ester), polyethylene glycol ether (PEG-ether), and polyglycerol ester (PG-ester) surfactants were prepared by solvent diffusion method. NLCs were characterized regarding particle size, polydispersity index, and zeta potential. Biocompatibility of NLCs was assessed on Caco-2 cells via resazurin assay. In vitro lipolysis study was performed using a standard lipid digestion method. Proteolytic studies were performed in simulated gastric fluid containing pepsin and simulated intestinal fluid containing pancreatin. Lipophilicity of insulin in terms of log Poctanol/water was improved from −1.8 to 2.1. NLCs were in the size range of 64–217 nm with a polydispersity index of 0.2–0.5 and exhibited a negative surface charge. PG-ester NLCs were non-cytotoxic up to a concentration of 0.5%, PEG-ester NLCs up to a concentration of 0.25% and PEG-ether NLC up to a concentration of 0.125% (w/v). The lipolysis study showed the release of >90%, 70%, and 10% of free fatty acids from PEG-ester, PG-ester, and PEG-ether NLCs, respectively. Proteolysis results revealed the highest protective effect of PEG-ether NLCs followed by PG-ester and PEG-ester NLCs for incorporated insulin complex. Findings suggest that NLCs bearing substructures less susceptible to degrading enzymes on their surface can provide higher protection for incorporated peptides toward gastrointestinal proteases.
Recent Progress in Drug Release Testing Methods of Biopolymeric Particulate System
Authors: Kim Yejin , Park Eun Ji , Kim Tae Wan , Na Dong Hee
Published: August 2021

Biopolymeric microparticles have been widely used for long-term release formulations of short half-life chemicals or synthetic peptides. Characterization of the drug release from microparticles is important to ensure product quality and desired pharmacological effect. However, there is no official method for long-term release parenteral dosage forms. Much work has been done to develop methods for in vitro drug release testing, generally grouped into three major categories: sample and separate, dialysis membrane, and continuous flow (flow-through cell) methods. In vitro drug release testing also plays an important role in providing insight into the in vivo performance of a product. In vitro release test with in vivo relevance can reduce the cost of conducting in vivo studies and accelerate drug product development. Therefore, investigation of the in vitro–in vivo correlation (IVIVC) is increasingly becoming an essential part of particulate formulation development. This review summarizes the principles of the in vitro release testing methods of biopolymeric particulate system with the recent research articles and discusses their characteristics including IVIVC, accelerated release testing methods, and stability of encapsulated drugs.
Metabolism of Diterpenoids Derived from the Bark of Cinnamomum cassia in Human Liver Microsomes
Authors: Choi Su Min , Pham Van Cong , Lee Sangkyu , Kim Jeong Ah
Published: August 2021

Cinnamomum cassia L. is used as a spice and flavoring agent as well as a traditional medicine worldwide. Diterpenoids, a class of compounds present in C. cassia, have various pharmacological effects, such as anti-inflammatory, antitumor, and antibacterial activities; however, there are insufficient studies on the metabolism of diterpenoids. In this study, the metabolism of seven diterpenoids, namely, anhydrocinnzeylanol, anhydrocinnzeylanine (AHC), cinncassiol A, cinncassiol B, cinnzeylanol, cinnzeylanone, and cinnzeylanine, obtained from the bark of C. cassia was studied in human liver microsomes (HLMs). All studied diterpenoids, except for AHC, exhibited strong metabolic stability; however, AHC was rapidly metabolized to 3% in HLMs in the presence of β-NADPH. Using a high-resolution quadrupole-orbitrap mass spectrometer, 20 metabolites were identified as dehydrogenated metabolites (M1–M3), dehydrogenated and oxidated metabolites (M4–M10), mono-oxidated metabolites (M11–M13), or dioxidated metabolites (M14–M20). In addition, CYP isoforms involved in AHC metabolism were determined by profiling metabolites produced after incubation in 11 recombinant cDNA-expressed CYP isoforms. Thus, the diterpenoid compound AHC was identified in a metabolic pathway involving CYP3A4 in HLMs.
Formulation Considerations for Autologous T Cell Drug Products
Authors: van der Walle Christopher , Godbert Sonya , Saito Gabriele , Azhari Zein
Published: August 2021

Genetically modified autologous T cells have become an established immunotherapy in the fight against cancer. The manufacture of chimeric antigen receptor (CAR) and αβ-T cell receptor (TCR) transduced T cells poses unique challenges, including the formulation, cryopreservation and fill–finish steps, which are the focus of this review. With an increasing number of marketing approvals for CAR-T cell therapies, comparison of their formulation design and presentation for administration can be made. These differences will be discussed alongside the emergence of automated formulation and fill-finish processes, the formulation design space, Monte Carlo simulation applied to risk analysis, primary container selection, freezing profiles and thaw and the use of dimethyl sulfoxide and alternative solvents/excipients as cryopreservation agents. The review will conclude with a discussion of the pharmaceutical solutions required to meet the simplification of manufacture and flexibility in dosage form for clinical treatment.
A Review and Evaluation of the Data Supporting Internal Use of Helichrysum italicum
Authors: Kramberger Katja , Kenig Saša , Jenko Pražnikar Zala , Kočevar Glavač Nina , Barlič-Maganja Darja
Published: August 2021

Helichrysum italicum is a Mediterranean plant with various pharmacological activities. Despite extensive reports on the bioactivity of the plant, its clinically studied applications have not yet been reviewed. The aim of our study was to gather information on the internal use of H. italicum and its bioactive constituents to determine its efficacy and safety for human use. We reviewed research articles that have not been previously presented in this context and analyzed relevant clinical studies with H. italicum. Cochranelibrary.com revealed six eligible clinical trials with H. italicum that examined indications for pain management, cough, and mental exhaustion. Although the efficacy of H. italicum has been demonstrated both in in vitro tests and in humans, it is difficult to attribute results from clinical trials to H. italicum alone, as it has usually not been tested as the sole component. On the other hand, clinical trials provide positive information on the safety profile since no adverse effects have been reported. We conclude that H. italicum is safe to use internally, while new clinical studies with H. italicum as a single component are needed to prove its efficacy. Based on the recent trend in H. italicum research, further studies are to be expected.
Pre- and/or Postharvest Silicon Application Prolongs the Vase Life and Enhances the Quality of Cut Peony (Paeonia lactiflora Pall.) Flowers
Authors: Song Jinnan , Li Yali , Hu Jiangtao , Lee Jaehyeok , Jeong Byoung Ryong
Published: August 2021

Peony is an important ornamental plant and has become increasingly popular for cut flower cultivation. However, a short vase life and frequent poor vase quality severely restrict its market value. The study described herein was conducted to investigate the effects of silicon application on the vase life and quality of two cut peony (Paeonia lactiflora Pall.) cultivars, ‘Taebaek’ and ‘Euiseong’. For pre- and/or postharvest silicon application, four experimental groups based on treatments were designed. With silicon treatment, the relevant growth attributes, including the shoot and leaf lengths, stem and bud diameters as well as the leaf width were all remarkably increased. In the postharvest storage, the addition of silicon to the holding solution in the vase was able to significantly extend vase life, delay fresh weight decrease, and improve vase quality, as characterized by the antioxidant enzyme activities and mechanical stem strength. Taken together, silicon application, regardless of the approach, was able to effectively prolong the vase life and enhance the quality of cut peony flowers.
P2K1 Receptor, Heterotrimeric Gα Protein and CNGC2/4 Are Involved in Extracellular ATP-Promoted Ion Influx in the Pollen of Arabidopsis thaliana
Authors: Wu Yansheng , Yin Hongmin , Liu Xinyue , Xu Jiawei , Qin Baozhi , Feng Kaili , Kang Erfang , Shang Zhonglin
Published: August 2021

As an apoplastic signal, extracellular ATP (eATP) is involved in plant growth and development. eATP promotes tobacco pollen germination (PG) and pollen tube growth (PTG) by stimulating Ca2+ or K+ absorption. Nevertheless, the mechanisms underlying eATP-stimulated ion uptake and their role in PG and PTG are still unclear. Here, ATP addition was found to modulate PG and PTG in 34 plant species and showed a promoting effect in most of these species. Furthermore, by using Arabidopsis thaliana as a model, the role of several signaling components involved in eATP-promoted ion (Ca2+, K+) uptake, PG, and PTG were investigated. ATP stimulated while apyrase inhibited PG and PTG. Patch-clamping results showed that ATP promoted K+ and Ca2+ influx into pollen protoplasts. In loss-of-function mutants of P2K1 (dorn1-1 and dorn1-3), heterotrimeric G protein α subunit (gpa1-1, gpa1-2), or cyclic nucleotide gated ion channel (cngc2, cngc4), eATP-stimulated PG, PTG, and ion influx were all impaired. Our results suggest that these signaling components may be involved in eATP-promoted PG and PTG by regulating Ca2+ or K+ influx in Arabidopsis pollen grains.
Influence of Dental Glass Fibers and Orthopedic Mesh on the Failure Loads of Polymethyl Methacrylate Denture Base Resin
Authors: Rana Muhammad H. , Shaik Sharaz , Hameed Mohammad S. , Al-Saleh Samar , AlHamdan Eman M. , Alshahrani Abdullah , Alqahtani Abdulaziz , Albaqawi Ahmed Heji , Vohra Fahim , Abduljabbar Tariq
Published: August 2021

The aim of the present study was to evaluate the fracture loads of polymethyl methacrylate (PMMA) complete denture bases reinforced with glass-fiber mesh and orthopedic casting tape (OCT) in comparison to conventional PMMA dentures under artificial aging. Dental fiberglass framework (Group 1) and OCT (Group 2 and 3) reinforced PMMA acrylic dentures were fabricated on the edentulous ridge. Ten PMMA dentures without reinforcement (Group 4) were included as controls. All specimens were placed in a chewing simulator chamber, and fatigue load was applied. To assess the fracture loads, static loads with a universal testing machine were applied. Fractured specimens in each group were evaluated under a scanning electron microscope. The data were statistically analyzed employing analysis of variance and Tukey post-hoc test. The association of denture weight and thickness on fracture load was assessed using Pearson and Spearman correlations. Dental fiberglass (Group 1) displayed the highest fracture load (692.33 ± 751.41 N), and Group 4 (control) exhibited the lowest fracture loads (281.41 ± 302.51 N). Dentures reinforced with fiberglass mesh framework exhibited intact fractures. In contrast, Group 2 and 3 specimens using OCT demonstrated ditching fractures. It was observed that the thickness and weight of all the reinforced specimens influenced the load required to fracture the dentures (p < 0.001). Denture specimens strengthened with OCT (Groups 2 and 3) exhibited failure loads lower than dental fiberglass (Group 1) specimens but higher than unreinforced controls.
Conformational Behavior, Topographical Features, and Antioxidant Activity of Partly De-Esterified Arabinoxylans
Authors: De Anda-Flores Yubia , Carvajal-Millan Elizabeth , Lizardi-Mendoza Jaime , Rascon-Chu Agustin , Tanori-Cordova Judith , Martínez-López Ana Luisa , Burgara-Estrella Alexel J. , Pedroza-Montero Martin R.
Published: August 2021

This study aimed to investigate the effect of arabinoxylans (AX) partial de-esterification with feruloyl esterase on the polysaccharide conformational behavior, topographical features, and antioxidant activity. After enzyme treatment, the ferulic acid (FA) content in AX was reduced from 7.30 to 5.48 µg FA/mg polysaccharide, and the molecule registered a small reduction in radius of gyration (RG), hydrodynamic radius (Rh), characteristic ratio (C∞), and persistence length (q). A slight decrease in α and a small increase in K constants in the Mark–Houwink–Sakurada equation for partially de-esterified AX (FAX) suggested a reduction in molecule structural rigidity and a more expanded coil conformation, respectively, in relation to AX. Fourier transform infrared spectroscopy spectra of AX and FAX presented a pattern characteristic for this polysaccharide. Atomic force microscopy topographic analysis of FAX showed a more regular surface without larger hollows in relation to AX. The antioxidant activity of FAX, compared to AX, was reduced by 30 and 41% using both 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods, respectively. These results suggest that feruloyl esterase treatment of AX could offer a strategy to tailor AX chains conformation, morphological features, and antioxidant activity, impacting the development of advanced biomaterials for biomedical and pharmaceutical applications.
Recent Advances in Design and Preparation of Polymer-Based Thermal Management Material
Authors: Zhang Hongli , Shi Tiezhu , Ma Aijie
Published: August 2021

The boosting of consumer electronics and 5G technology cause the continuous increment of the power density of electronic devices and lead to inevitable overheating problems, which reduces the operation efficiency and shortens the service life of electronic devices. Therefore, it is the primary task and a prerequisite to explore innovative material for meeting the requirement of high heat dissipation performance. In comparison with traditional thermal management material (e.g., ceramics and metals), the polymer-based thermal management material exhibit excellent mechanical, electrical insulation, chemical resistance and processing properties, and therefore is considered to be the most promising candidate to solve the heat dissipation problem. In this review, we summarized the recent advances of two typical polymer-based thermal management material including thermal-conduction thermal management material and thermal-storage thermal management material. Furtherly, the structural design, processing strategies and typical applications for two polymer-based thermal management materials were discussed. Finally, we proposed the challenges and prospects of the polymer-based thermal management material. This work presents new perspectives to develop advanced processing approaches and construction high-performance polymer-based thermal management material.
NOA61-Polymer Fiber Fizeau Interferometer with a Flexible NOA65-Polymer Taper for Simultaneous Measurement of Tilt Angle and Temperature
Authors: Lee Cheng-Ling , Chen Chi-Shiang , Yang Chun-Ren , Zeng Rui-Cheng
Published: August 2021

This study proposes and experimentally demonstrates a NOA61-polymer fiber Fizeau interferometer (PFFI) connected to a flexible NOA65-polymer taper (PT) for simultaneous measurement of tilt angle and temperature (T). The PT/PFFI fiber sensor consists of a taper-shaped flexible NOA65 polymer and single-mode fiber with an endface that is attached to a NOA61-polymer. The NOA61-polymer of PFFI is highly sensitive to variations of T with high repeatability and enables the simultaneous measurement of tilt angle by connecting with the highly flexible NOA65-PT. the interference fringe visibility of optical spectra in the PFFI can be highly controlled by the tilt angle of the PT and is thus capable of measuring tilt angles with high sensitivity. On the other hand, wavelength shifts of the spectra in the PFFI only occur when T varies. The proposed PT/PFFI can simultaneously detect the tilt state and the variation of surrounding T by measuring the optical spectral responses and eliminating cross sensitivity. Experimental results demonstrate the PT/PFFI can simultaneously measure tilt angles and T with good sensitivities and obtain averages of 0.4 dB/° and 0.17 nm/°C, respectively.
Charge Modification as a Mechanism for Tunable Properties in Polymer–Surfactant Complexes
Authors: Hill Christopher , Abdullahi Wasiu , Dalgliesh Robert , Crossman Martin , Griffiths Peter Charles
Published: August 2021

Oppositely charged polymer–surfactant complexes are frequently explored as a function of phase space defined by the charge ratio Z, (where Z = [+polymer]/[−surfactant]), commonly accessed through the surfactant concentration. Tuning the phase behaviour and related properties of these complexes is an important tool for optimising commercial formulations; hence, understanding the relationship between Z and bulk properties is pertinent. Here, within a homologous series of cationic hydroxyethyl cellulose (cat-HEC) polymers with minor perturbations in the degree of side chain charge modification, phase space is instead explored through [+polymer] at fixed Cpolymer. The nanostructures were characterised by small-angle neutron scattering (SANS) in D2O solutions and in combination with the oppositely charged surfactant sodium dodecylsulfate (h- or d-SDS). Scattering consistent with thin rods with an average radius of ∼7.7 Å and length of ∼85 Å was observed for all cat-HEC polymers and no significant interactions were shown between the neutral HEC polymer and SDS (CSDS < CMC). For the charge-modified polymers, interactions with SDS were evident and the radius of the formed complexes grew up to ∼15 Å with increasing Z. This study demonstrates a novel approach in which the Z phase space of oppositely charged polymer–surfactant complexes can be controlled at fixed concentrations.
Influence of Citric Acid on the Bond Strength of Beech Wood
Authors: Mihulja Goran , Živković Vjekoslav , Poljak Dominik , Šefc Bogoslav , Sedlar Tomislav
Published: August 2021

In this study, beech wood (Fagus silvatica L.) has been chemically modified with citric acid (Acidum citricum) and sodium hypophosphate (SHP) as the catalyst and gradually thermo-condensed in the dryer. Afterwards, wetting angle, surface energy, and shear strength of glued joints of modified and unmodified wood were determined. Testing of the bond strength according to standard EN 204 and comparison between modified and unmodified samples were executed. The adhesive used for bonding samples was polyvinyl acetate (PVAC), commonly used for gluing solid wood panels. Testing material was divided into three groups (dry, wet, and wet conditioned samples), within which statistical analysis was performed, and the significance of the differences between the modified and unmodified samples was determined. Surface energy is correlated with the bond strength, indicating that modification with citric acid negatively affects the adhesive properties of beech wood. A reduction in the bond strength of modified wood glued with PVAC glue compared to unmodified wood was determined. All the results indicate that the modified samples do not meet the minimum requirements for EN 204 bonded with PVAC glue. Therefore, it will be necessary to conduct further studies using other types of adhesives to investigate whether modified wood might be suitable for gluing.
Shear-Thinning in Oligomer Melts—Molecular Origins and Applications
Authors: Datta Ranajay , Yelash Leonid , Schmid Friederike , Kummer Florian , Oberlack Martin , Lukáčová-Medvid’ová Mária , Virnau Peter
Published: August 2021

We investigate the molecular origin of shear-thinning in melts of flexible, semiflexible and rigid oligomers with coarse-grained simulations of a sheared melt. Entanglements, alignment, stretching and tumbling modes or suppression of the latter all contribute to understanding how macroscopic flow properties emerge from the molecular level. In particular, we identify the rise and decline of entanglements with increasing chain stiffness as the major cause for the non-monotonic behaviour of the viscosity in equilibrium and at low shear rates, even for rather small oligomeric systems. At higher shear rates, chains align and disentangle, contributing to shear-thinning. By performing simulations of single chains in shear flow, we identify which of these phenomena are of collective nature and arise through interchain interactions and which are already present in dilute systems. Building upon these microscopic simulations, we identify by means of the Irving–Kirkwood formula the corresponding macroscopic stress tensor for a non-Newtonian polymer fluid. Shear-thinning effects in oligomer melts are also demonstrated by macroscopic simulations of channel flows. The latter have been obtained by the discontinuous Galerkin method approximating macroscopic polymer flows. Our study confirms the influence of microscopic details in the molecular structure of short polymers such as chain flexibility on macroscopic polymer flows.
Hydrophobic Modification of Chitosan via Reactive Solvent-Free Extrusion
Authors: Akopova Tatiana A. , Demina Tatiana S. , Khavpachev Mukhamed A. , Popyrina Tatiana N. , Grachev Andrey V. , Ivanov Pavel L. , Zelenetskii Alexander N.
Published: August 2021

Hydrophobic derivatives of polysaccharides possess an amphiphilic behavior and are widely used as rheological modifiers, selective sorbents, and stabilizers for compositions intended for various applications. In this work, we studied the mechanochemical reactions of chitosan alkylation when interacting with docosylglycidyl and hexadecylglycidyl ethers in the absence of solvents at shear deformation in a pilot twin-screw extruder. The chemical structure and physical properties of the obtained derivatives were characterized by elemental analysis, FT-IR spectroscopy, dynamic light scattering, scanning electron microscopy, and mechanical tests. According to calculations for products soluble in aqueous media, it was possible to introduce about 5–12 hydrophobic fragments per chitosan macromolecule with a degree of polymerization of 500–2000. The length of the carbon chain of the alkyl substituent significantly affects its reactivity under the chosen conditions of mechanochemical synthesis. It was shown that modification disturbs the packing ability of the macromolecules, resulting in an increase of plasticity and drop in the elastic modulus of the film made from the hydrophobically modified chitosan samples.
Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films
Authors: Zheng Yifan , Pierce Aidan F. , Wagner Willi L. , Khalil Hassan A. , Chen Zi , Funaya Charlotta , Ackermann Maximilian , Mentzer Steven J.
Published: August 2021

Anastomotic leakage is a frequent complication of intestinal surgery and a major source of surgical morbidity. The timing of anastomotic failures suggests that leaks are the result of inadequate mechanical support during the vulnerable phase of wound healing. To identify a biomaterial with physical and mechanical properties appropriate for assisted anastomotic healing, we studied the adhesive properties of the plant-derived structural heteropolysaccharide called pectin. Specifically, we examined high methoxyl citrus pectin films at water contents between 17–24% for their adhesivity to ex vivo porcine small bowel serosa. In assays of tensile adhesion strength, pectin demonstrated significantly greater adhesivity to the serosa than either nanocellulose fiber (NCF) films or pressure sensitive adhesives (PSA) (p < 0.001). Similarly, in assays of shear resistance, pectin demonstrated significantly greater adhesivity to the serosa than either NCF films or PSA (p < 0.001). Finally, the pectin films were capable of effectively sealing linear enterotomies in a bowel simulacrum as well as an ex vivo bowel segment. We conclude that pectin is a biomaterial with physical and adhesive properties capable of facilitating anastomotic healing after intestinal surgery.
Bioinspired Design of Sericin/Chitosan/Ag@MOF/GO Hydrogels for Efficiently Combating Resistant Bacteria, Rapid Hemostasis, and Wound Healing
Authors: Zhang Meng , Wang Dong , Ji Nana , Lee Shaoxiang , Wang Guohui , Zheng Yuqi , Zhang Xin , Yang Lin , Qin Zhiwei , Yang Yang
Published: August 2021

Due to the spread of drug-resistant bacteria in hospitals, the development of antibacterial dressings has become a strategy to control wound infections caused by bacteria. Here, we reported a green strategy for in situ biomimetic syntheses of silver nanoparticles@organic frameworks/graphene oxide (Ag@MOF–GO) in sericin/chitosan/polyvinyl alcohol hydrogel. Ag@MOF–GO was synthesized in situ from the redox properties of tyrosine residues in silk sericin without additional chemicals, similar to a biomineralization process. The sericin/chitosan/Ag@MOF–GO dressing possessed a high porosity, good water retention, and a swelling ratio. The hemolysis rate of the composite was 3.9% and the cell viability rate was 131.2%, which indicated the hydrogel possessed good biocompatibility. The composite also showed excellent lasting antibacterial properties against drug-sensitive and drug-resistant pathogenic bacteria. The composite possessed excellent hemostatic activity. The coagulation effect of the composite may be related to its effect on the red blood cells and platelets, but it has nothing to do with the activation of coagulation factors. An in vitro cell migration assay confirmed and an in vivo evaluation of mice indicated that the composite could accelerate wound healing and re-epithelialization. In summary, the composite material is an ideal dressing for accelerating hemostasis, preventing bacterial infection, and promoting wound healing.
The Influence of Modified Experimental Dental Resin Composites on the Initial In Situ Biofilm—A Triple-Blinded, Randomized, Controlled Split-Mouth Trial
Authors: Burgard Niklas , Kienitz Melanie , Jourdan Claudia , Rüttermann Stefan
Published: August 2021

The purpose of the study was to investigate the bacterial viability of the initial biofilm on the surface of experimental modified dental resin composites. Twenty-five healthy individuals with good oral hygiene were included in this study. In a split-mouth design, they received acrylic splints with five experimental composite resin specimens. Four of them were modified with either a novel polymeric hollow-bead delivery system or methacrylated polymerizable Irgasan (Antibacterial B), while one specimen served as an unmodified control (ST). A delivery system based on Poly-Pore® was loaded with one of the active agents: Tego® Protect 5000 (Antiadhesive A), Dimethicone (Antiadhesive B), or Irgasan (Antibacterial A). All study subjects refrained from toothbrushing during the study period. Specimens were detached from the splints after 8 h and given a live/dead staining before fluorescence microscopy. A Friedman test and a post hoc Nemenyi test were applied with a significance level at p < 0.05. In summary, all materials but Antibacterial B showed a significant antibacterial effect compared to ST. The results suggested the role of the materials’ chemistry in the dominance of cell adhesion. In conclusion, dental resin composites with Poly-Pore-loaded active agents showed antibacterial effectiveness in situ.
A Comprehensive Review of Microneedles: Types, Materials, Processes, Characterizations and Applications
Authors: Aldawood Faisal Khaled , Andar Abhay , Desai Salil
Published: August 2021

Drug delivery through the skin offers many advantages such as avoidance of hepatic first-pass metabolism, maintenance of steady plasma concentration, safety, and compliance over oral or parenteral pathways. However, the biggest challenge for transdermal delivery is that only a limited number of potent drugs with ideal physicochemical properties can passively diffuse and intercellularly permeate through skin barriers and achieve therapeutic concentration by this route. Significant efforts have been made toward the development of approaches to enhance transdermal permeation of the drugs. Among them, microneedles represent one of the microscale physical enhancement methods that greatly expand the spectrum of drugs for transdermal and intradermal delivery. Microneedles typically measure 0.1–1 mm in length. In this review, microneedle materials, fabrication routes, characterization techniques, and applications for transdermal delivery are discussed. A variety of materials such as silicon, stainless steel, and polymers have been used to fabricate solid, coated, hollow, or dissolvable microneedles. Their implications for transdermal drug delivery have been discussed extensively. However, there remain challenges with sustained delivery, efficacy, cost-effective fabrication, and large-scale manufacturing. This review discusses different modes of characterization and the gaps in manufacturing technologies associated with microneedles. This review also discusses their potential impact on drug delivery, vaccine delivery, disease diagnostic, and cosmetics applications.
Exohedral Functionalization of Fullerene by Substituents Controlling of Molecular Organization for Spontaneous C60 Dimerization in Liquid Crystal Solutions and in a Bulk Controlled by a Potential
Authors: Czichy Malgorzata , Colombo Alessia , Wagner Pawel , Janasik Patryk , Dragonetti Claudia , Raja Rathinam , Officer David L. , Wang Leeyih
Published: August 2021

A study was carried out on the possibility of orderly and spontaneous dimerization at room temperature of C60 cages in fullerene liquid crystal fullerene dyads (R-C60). For this purpose, dyads with a structural elements feature supporting π-stacking and Van der Waals interactions were tested, due to the presence of terthiophene donors linked through an α-position or dodecyloxy chains. In addition, this possibility was also tested and compared to dyads with shorter substituents and the pristine C60. Research has shown that only in dyads with the features of liquid crystals, π-dimerization of C60 units occurs, which was verified by electrochemical and spectroelectrochemical (ESR) measurements. Cyclic voltammetry and differential voltammetry studies reveal π-dimerization in liquid crystal dyad solution even without the possibility of previous polymerization (cathodic or anodic) under conditions in the absence of irradiation and without the availability of reaction initiators, and even with the use of preliminary homogenization. These dyads undergo six sequential, one-electron reductions of π-dimer (R-C60···C60-R), where two electrons are added successively to each of the two fullerene cages and first form two radical anion system (R-C60)•−(R-C60)•− without pairing with the characteristics of two doublets. Similarly, the second reductions of π-dimer occur at potentials that are close to the reduction potential for the conversion to a system of two triplet dianions (R-C60)2−(R-C60)2−. Electron paramagnetic resonance spectra indicate a significant interaction between C60 cages. Interestingly, the strength of intermolecular bonds is so significant that it can overcome Coulombic repulsion, even with such highly charged particles as dianions and trianions. Such behavior has been revealed and studied so far only in covalently bonded C60 dimers.
Blended Anion Exchange Membranes for Vanadium Redox Flow Batteries
Authors: Son Tae Yang , Im Kwang Seop , Jung Ha Neul , Nam Sang Yong
Published: August 2021

In this study, blended anion exchange membranes were prepared using polyphenylene oxide containing quaternary ammonium groups and polyvinylidene fluoride. A polyvinylidene fluoride with high hydrophobicity was blended in to lower the vanadium ion permeability, which increased when the hydrophilicity increased. At the same time, the dimensional stability also improved due to the excellent physical properties of polyvinylidene fluoride. Subsequently, permeation of the vanadium ions was prevented due to the positive charge of the anion exchange membrane, and thus the permeability was relatively lower than that of a commercial proton exchange membrane. Due to the above properties, the self-discharge of the blended anion exchange membrane (30.1 h for QA–PPO/PVDF(2/8)) was also lower than that of the commercial proton exchange membrane (27.9 h for Nafion), and it was confirmed that it was an applicable candidate for vanadium redox flow batteries.
The State of the Art of Material Jetting—A Critical Review
Authors: Gülcan Orhan , Günaydın Kadir , Tamer Aykut
Published: August 2021

Material jetting (MJ) technology is an additive manufacturing method that selectively cures liquid photopolymer to build functional parts. The use of MJ technology has increased in popularity and been adapted by different industries, ranging from biomedicine and dentistry to manufacturing and aviation, thanks to its advantages in printing parts with high dimensional accuracy and low surface roughness. To better understand the MJ technology, it is essential to address the capabilities, applications and the usage areas of MJ. Additionally, the comparison of MJ with alternative methods and its limitations need to be explained. Moreover, the parameters influencing the dimensional accuracy and mechanical properties of MJ printed parts should be stated. This paper aims to review these critical aspects of MJ manufacturing altogether to provide an overall insight into the state of the art of MJ.
Experimental Characterization and Modeling Multifunctional Properties of Epoxy/Graphene Oxide Nanocomposites
Authors: Naresh Kakur , Khan Kamran A. , Umer Rehan
Published: August 2021

Thermomechanical modeling of epoxy/graphene oxide under quasi-static and dynamic loading requires thermo-mechanical properties such as Young’s modulus, Poisson’s ratio, thermal conductivity, and frequency-temperature dependent viscoelastic properties. In this study, the effects of different graphene oxide (GO) concentrations (0.05, 0.1, and 0.2 wt%) within an epoxy matrix on several mechanical and thermal properties were investigated. The distribution of GO fillers in the epoxy was investigated using transmission electron microscopy (TEM). The digital image correlation (DIC) technique was employed during the tensile testing to determine Young’s modulus and Poisson’s ratio. Analytical models were used to predict Young’s modulus and thermal conductivity, with an error of less than 13% and 9%, respectively. Frequency–temperature dependent phenomenological models were proposed to predict the storage moduli and loss tangent, with a reasonable agreement with experimental data. A relatively high storage modulus, heat-resistance index (THRI), and thermal conductivity were observed in 0.2 wt% nanocomposite samples compared with pure epoxy and other lower concentration GO nanocomposites. A high THRI and derivative of thermogravimetric analysis peak temperatures (Tm1 and Tm2) were exhibited by adding nano-fillers in the epoxy, which confirms higher thermal stability of nanocomposites than that of pristine epoxy.
Tuyere-Level Syngas Injection in the Blast Furnace: A Computational Fluid Dynamics Investigation
Authors: Nielson Samuel , Okosun Tyamo , Damstedt Bradley , Jampani Megha , Zhou Chenn Q.
Published: August 2021

With the recent push towards high injection rate blast furnace operation for economic and environmental reasons, it has become desirable in North America to better understand the impacts of alternate injected gas fuels in comparison to the well-documented limitations of natural gas. The quenching effects of gas injection on the furnace present a functional limit on the maximum stable injection rate which can be utilized. With this in mind, researchers at Purdue University Northwest’s Center for Innovation through Visualization and Simulation utilized previously developed computational fluid dynamics (CFD) models of the blast furnace to explore the impacts of replacing natural gas with syngas in a blast furnace with a single auxiliary fuel supply. Simulations predicted that the syngas injection can indeed reduce coke consumption in the blast furnace at similar injection rates to natural gas while maintaining stable raceway flame and reducing gas temperatures. The coke rates predicted by modeling using similar injection rates indicated an improvement of 8 to 15 kg/thm compared to baseline conditions when using the syngas of various feedstocks. Additionally, syngas injection scenarios typically produced higher raceway flame temperatures than comparable natural gas injection cases, indicating potential headroom for reducing oxygen enrichment in the hot blast or providing an even higher total injection rate.
Design and Performance Test of the Coffee Bean Classifier
Authors: Ansar  , Sukmawaty  , Murad  , Muttalib Surya Abdul , Putra Riyan Hadi , Abdurrahim
Published: August 2021

Currently, some coffee production centers still perform classification manually, which requires a very long time, a lot of labor, and expensive operational costs. Therefore, the purpose of this research was to design and test the performance of a coffee bean classifier that can accelerate the process of classifying beans. The classifier used consisted of three main parts, namely the frame, the driving force, and sieves. The research parameters included classifier work capacity, power, specific energy, classification distribution and effectiveness, and efficiency. The results showed that the best operating conditions of the coffee bean classifier was a rotational speed of 91.07 rpm and a 16° sieve angle with a classifier working capacity of 38.27 kg/h: the distribution of the seeds retained in the first sieve was 56.77%, the second sieve was 28.12%, and the third sieve was 15.11%. The efficiency of using a classifier was found at a rotating speed of 91.07 rpm and a sieve angle of 16°. This classifier was simple in design, easy to operate, and can sort coffee beans into three classifications, namely small, medium, and large.
The Long Road Out of Eden: Early Dynastic Temples, a Quantitative Approach to the Bent-Axis Shrines
Authors: Butterlin Pascal
Published: August 2021

This study was conducted to quantitatively assess the architectural data stemming from 70 buildings usually considered as bent-axis temples, a type of Mesopotamian temple mainly constructed from 2900 to 2300–2200 BC. The study reviews, region-by-region and site-by-site, the dimensions of the rooms considered the “holy of holies”, registering width, length, and surface area. The results are discussed in comparison to the previous reception rooms of the tripartite buildings, considered the original matrix from which these shrines developed. The chronological and regional differences that are outlined provide some insights about the kind of social units that were involved in the use of those buildings, which were key structures in the urban fabric of Early City states.
A Balinese ‘Call to Prayer’: Sounding Religious Nationalism and Local Identity in the Puja Tri Sandhya
Authors: Hynson Meghan
Published: August 2021

This article examines the Puja Tri Sandhya, a Balinese Hindu prayer that has been broadcast into the soundscape of Bali since 2001. By charting the development of the prayer, this paper summarizes the religious politics of post-independence Indonesia, which called for the Balinese to adopt the Puja Tri Sandhya as a condition for religious legitimacy in the new nation. The Puja Tri Sandhya is likened to a Balinese “call to prayer” and compared to Muslim and Christian soundings of religion in the archipelago to assert how these broadcasts sonically reify the national motto, Bhinneka Tunggal Ika (“Unity in Diversity”), and participate in a sounding of religious nationalism. Although these broadcasts are evidence of a state-sponsored form of religiosity, interviews concerning the degree to which individuals practice the Puja Tri Sandhya point to an element of secularism and position the prayer as an example that challenges the religion versus secularism dichotomy in studies of religious nationalism. This article also examines the sonic components of the Puja Tri Sandhya (when it is sounded, the vocal style, and the gender wayang and genta bell accompaniment), to argue how these elements infuse this invented display of religiosity with authority and facilitate a mediation between technology, space, and local identity. Exploration of the gender wayang accompaniment in particular, further confirms the contrived nature of the Puja Tri Sandhya and demonstrates how technologies used to broadcast the prayer have had a significant impact on the gender wayang musical tradition.
IONORING: Real-Time Monitoring of the Total Electron Content over Italy
Authors: Cesaroni Claudio , Spogli Luca , De Franceschi Giorgiana
Published: August 2021

IONORING (IONOspheric RING) is a tool capable to provide the real-time monitoring and modeling of the ionospheric Total Electron Content (TEC) over Italy, in the latitudinal and longitudinal ranges of 35°N–48°N and 5°E–20°E, respectively. IONORING exploits the Global Navigation Satellite System (GNSS) data acquired by the RING (Rete Integrata Nazionale GNSS) network, managed by the Istituto Nazionale di Geofisica e Vulcanologia (INGV). The system provides TEC real-time maps with a very fine spatial resolution (0.1° latitude x 0.1° longitude), with a refresh time of 10 min and a typical latency below the minute. The TEC estimated at the ionospheric piercing points from about 40 RING stations, equally distributed over the Italian territory, are interpolated using locally (weighted) regression scatter plot smoothing (LOWESS). The validation is performed by comparing the IONORING TEC maps (in real-time) with independent products: (i) the Global Ionospheric Maps (GIM) - final product- provided by the International GNSS Service (IGS), and (ii) the European TEC maps from the Royal Observatory of Belgium. The validation results are satisfactory in terms of Root Mean Square Error (RMSE) between 2 and 3 TECu for both comparisons. The potential of IONORING in depicting the TEC daily and seasonal variations is analyzed over 3 years, from May 2017 to April 2020, as well as its capability to account for the effect of the disturbed geospace on the ionosphere at mid-latitudes. The IONORING response to the X9.3 flare event of September 2017 highlights a sudden TEC increase over Italy of about 20%, with a small, expected dependence on the latitude, i.e., on the distance from the subsolar point. Subsequent large regional TEC various were observed in response to related follow-on geomagnetic storms. This storm is also used as a case event to demonstrate the potential of IONORING in improving the accuracy of the GNSS Single Point Positioning. By processing data in kinematic mode and by using the Klobuchar as the model to provide the ionospheric correction, the resulting Horizontal Positioning Error is 4.3 m, lowering to, 3.84 m when GIM maps are used. If IONORING maps are used as the reference ionosphere, the error is as low as 2.5 m. Real-times application and services in which IONORING is currently integrated are also described in the conclusive remarks.