bluuebunny/crossref_metadata_2025 huggingface dataset:
Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes
Authors: Tu Long , Li Xuzhou , Bian Shengtai , Yu Yingting , Li Junxiang , Huang Liang , Liu Peng , Wu Qiong , Wang Wenhui
Published: September 2017

Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as the substrate perforated with periodic, near-cm2-area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. Variations in spectral shift during the same culture period across single cells present new evidence for cell heterogeneity. The micro-nano-device provides a new, label-free, real-time, and sensitive, platform to investigate the cell adhesion kinetics at single cell level.
The Oxidative State of Cysteine Thiol 144 Regulates the SIRT6 Glucose Homeostat
Authors: Long David , Wu Hanzhi , Tsang Allen W. , Poole Leslie B. , Yoza Barbara K. , Wang Xianfeng , Vachharajani Vidula , Furdui Cristina M. , McCall Charles E.
Published: September 2017

Control of glucose homeostasis plays a critical role in health and lifespan and its dysregulation contributes to inflammation, cancer and aging. NAD + dependent Sirtuin 6 (SIRT6) is a glucose homeostasis regulator in animals and humans and its regulation at the molecular level is unknown. Here, we report that a cysteine thiol redox sensor contributes to the role of SIRT6 in controlling glucose homeostasis. Sulfenylation of SIRT6 occurs in THP1 cells and primary human promonocytes during inflammation and in splenocytes from mice with sepsis. Inhibiting xanthine oxidase, a major reactive oxygen species (ROS) contributor during acute inflammation, reduces sulfenylation of SIRT6, glucose transporter Glut1 expression, glucose uptake, and glycolysis. A block in glycolysis associated with monocyte deactivation by endotoxin, a process contributing to immunometabolic paralysis in human and mouse sepsis monocytes, can be reversed by increasing H2O2 and sulfenylating SIRT6. Mutation analysis of SIRT6 Cys144, which lies in its phylogenetically conserved zinc-associated Cys-X-X-Cys motif near the catalytic domain of the protein, decreases SIRT6 deacetylase activity and promotes glycolysis. These results suggest that direct and reversible cysteine thiol 144 may play a functional role in SIRT6-dependent control over monocyte glycolysis, an important determinant of effector innate immune responses.
Simulation of head and neck cancer oxygenation and doubling time in a 4D cellular model with angiogenesis
Authors: Forster Jake C. , Douglass Michael J. J. , Harriss-Phillips Wendy M. , Bezak Eva
Published: September 2017

Tumor oxygenation has been correlated with treatment outcome for radiotherapy. In this work, the dependence of tumor oxygenation on tumor vascularity and blood oxygenation was determined quantitatively in a 4D stochastic computational model of head and neck squamous cell carcinoma (HNSCC) tumor growth and angiogenesis. Additionally, the impacts of the tumor oxygenation and the cancer stem cell (CSC) symmetric division probability on the tumor volume doubling time and the proportion of CSCs in the tumor were also quantified. Clinically relevant vascularities and blood oxygenations for HNSCC yielded tumor oxygenations in agreement with clinical data for HNSCC. The doubling time varied by a factor of 3 from well oxygenated tumors to the most severely hypoxic tumors of HNSCC. To obtain the doubling times and CSC proportions clinically observed in HNSCC, the model predicts a CSC symmetric division probability of approximately 2% before treatment. To obtain the doubling times clinically observed during treatment when accelerated repopulation is occurring, the model predicts a CSC symmetric division probability of approximately 50%, which also results in CSC proportions of 30–35% during this time.
Homogeneity and High Concordance of ALK Translocation in Primary Lung Adenocarcinoma and Paired Lymph Node Metastasis
Authors: Ma Wei , Guo Lei , Shan Ling , Liu Xiuyun , Lyu Ning , Ying Jianming
Published: September 2017

Translocation of anaplastic lymphoma kinase (ALK) gene is an important determinator for the response to ALK tyrosine kinase inhibitor (TKI) in non-small-cell lung cancer (NSCLC) patients. The existence of genetic heterogeneity will affect the results of molecular testing, especially in biopsy samples from primary or metastatic sites of patients with advanced stage NSCLC. We intended to explore the heterogeneity of ALK gene translocation in excision specimens and to examine the existence of discordance of ALK status between primary tumours and corresponding lymph node metastases. A total of 106 ALK positive lung adenocarcinoma cases were collected for assessment of intratumour heterogeneity of ALK gene translocation, which were stained by the fully automated Ventana ALK D5F3 immunohistochemistry (IHC) analysis. In addition, the ALK gene translocations were evaluated in a series of 53 primary tumours and their paired lymph node metastases using ALK D5F3 IHC staining. The concordance rate between primary tumours and paired metastatic lymph nodes was 100%. ALK status was homogeneous in lung adenocarcinoma samples and was generally stable during metastasis. Therefore, ALK gene translocation can be measured reliably in material from either primary or metastatic tumours in lung adenocarcinoma patients.
Antennal transcriptome and expression analyses of olfactory genes in the sweetpotato weevil Cylas formicarius
Authors: Bin Shu-Ying , Qu Meng-Qiu , Pu Xin-Hua , Wu Zhong-Zhen , Lin Jin-Tian
Published: September 2017

The sweetpotato weevil, Cylas formicarius (Fabricius), is a serious pest of sweetpotato. Olfaction-based approaches, such as use of synthetic sex pheromones to monitor populations and the bait-and-kill method to eliminate males, have been applied successfully for population management of C. formicarius. However, the molecular basis of olfaction in C. formicarius remains unknown. In this study, we produced antennal transcriptomes from males and females of C. formicarius using high-throughput sequencing to identify gene families associated with odorant detection. A total of 54 odorant receptors (ORs), 11 gustatory receptors (GRs), 15 ionotropic receptors (IRs), 3 sensory neuron membrane proteins (SNMPs), 33 odorant binding proteins (OBPs), and 12 chemosensory proteins (CSPs) were identified. Tissue-specific expression patterns revealed that all 54 ORs and 11 antennal IRs, one SNMP, and three OBPs were primarily expressed in antennae, suggesting their putative roles in olfaction. Sex-specific expression patterns of these antenna-predominant genes suggest that they have potential functions in sexual behaviors. This study provides a framework for understanding olfaction in coleopterans as well as future strategies for controlling the sweetpotato weevil pest.
Insight into shark magnetic field perception from empirical observations
Authors: Anderson James M. , Clegg Tamrynn M. , Véras Luisa V. M. V. Q. , Holland Kim N.
Published: September 2017

Elasmobranch fishes are among a broad range of taxa believed to gain positional information and navigate using the earth’s magnetic field, yet in sharks, much remains uncertain regarding the sensory receptors and pathways involved, or the exact nature of perceived stimuli. Captive sandbar sharks, Carcharhinus plumbeus were conditioned to respond to presentation of a magnetic stimulus by seeking out a target in anticipation of reward (food). Sharks in the study demonstrated strong responses to magnetic stimuli, making significantly more approaches to the target (p = < 0.01) during stimulus activation (S+) than before or after activation (S−). Sharks exposed to reversible magnetosensory impairment were less capable of discriminating changes to the local magnetic field, with no difference seen in approaches to the target under the S+ and S− conditions (p = 0.375). We provide quantified detection and discrimination thresholds of magnetic stimuli presented, and quantify associated transient electrical artefacts. We show that the likelihood of such artefacts serving as the stimulus for observed behavioural responses was low. These impairment experiments support hypotheses that magnetic field perception in sharks is not solely performed via the electrosensory system, and that putative magnetoreceptor structures may be located in the naso-olfactory capsules of sharks.
Impaired fibrinolysis and lower levels of plasma α2-macroglobulin are associated with an increased risk of severe asthma exacerbations
Authors: Bazan-Socha Stanislawa , Mastalerz Lucyna , Cybulska Agnieszka , Zareba Lech , Kremers Romy , Zabczyk Michal , Pulka Grazyna , Iwaniec Teresa , Bazan Jan G. , Hemker Coenraad , Undas Anetta
Published: September 2017

Recently we have reported that asthma is associated with enhanced plasma thrombin formation, impaired fibrinolysis and platelet activation. In the present study we investigated whether described prothrombotic blood alterations might predispose to thromboembolic events or asthma exacerbations. In 164 adult asthmatics we assessed clinical events during 3-year follow-up and analyzed their associations with measured at baseline prothrombotic blood parameters. Data were obtained from 157 (95.7%) of the asthma patients. We documented 198 severe asthma exacerbations (64/year), which occurred in 53 subjects (34%). These patients were older (p = 0.004), had worse asthma control (p = 0.02) and lower spirometry values (p = 0.01), at baseline. Interestingly, this subgroup had longer clot lysis time (CLT), as well as lower α2-macroglobulin (p = 0.038 and p = 0.04, respectively, after adjustment for potential confounders). Increased CLT and lower α2-macroglobulin were demonstrated as independent predictors of asthma exacerbation in multiple regression model. Moreover, we documented two episodes of deep vein thrombosis (1.3%), and eight acute coronary syndromes (5.1%). Patients who experienced thromboembolic events (n = 10, 6.4%, 2.1%/year) had lower α2-macroglobulin (p = 0.04), without differences in efficiency of fibrinolysis and thrombin generation. Impaired fibrinolysis and lower levels of α2-macroglobulin might predispose to a higher rate of asthma exacerbations, suggesting new links between disturbed hemostasis and asthma.
Voluntary liquorice ingestion increases blood pressure via increased volume load, elevated peripheral arterial resistance, and decreased aortic compliance
Authors: Hautaniemi Elina J. , Tahvanainen Anna M. , Koskela Jenni K. , Tikkakoski Antti J. , Kähönen Mika , Uitto Marko , Sipilä Kalle , Niemelä Onni , Mustonen Jukka , Pörsti Ilkka H.
Published: September 2017

We investigated the haemodynamic effects of two-week liquorice exposure (glycyrrhizin dose 290–370 mg/day) in 22 healthy volunteers during orthostatic challenge. Haemodynamics were recorded during passive 10-minute head-up tilt using radial pulse wave analysis, whole-body impedance cardiography, and spectral analysis of heart rate variability. Thirty age-matched healthy subjects served as controls. Liquorice ingestion elevated radial systolic (p < 0.001) and diastolic (p = 0.018) blood pressure and systemic vascular resistance (p = 0.037). During orthostatic challenge, heart rate increased less after the liquorice versus control diet (p = 0.003) and low frequency power of heart rate variability decreased within the liquorice group (p = 0.034). Liquorice intake increased central pulse pressure (p < 0.001) and augmentation index (p = 0.002) supine and upright, but in the upright position the elevation of augmentation index was accentuated (p = 0.007). Liquorice diet also increased extracellular fluid volume (p = 0.024) and aortic to popliteal pulse wave velocity (p = 0.027), and aortic characteristic impedance in the upright position (p = 0.002). To conclude, in addition to increased extracellular fluid volume and large arterial stiffness, two weeks of liquorice ingestion elevated systemic vascular resistance and augmentation index. Measurements performed at rest may underestimate the haemodynamic effects of liquorice ingestion, as enhanced central wave reflection and reduced chronotropic response were especially observed in the upright position.
Urban PM2.5 exacerbates allergic inflammation in the murine lung via a TLR2/TLR4/MyD88-signaling pathway
Authors: He Miao , Ichinose Takamichi , Yoshida Yasuhiro , Arashidani Keiichi , Yoshida Seiichi , Takano Hirohisa , Sun Guifan , Shibamoto Takayuki
Published: September 2017

Nevertheless its mechanism has not been well explained yet, PM2.5 is recognized to exacerbate asthma. In the present study, the roles of toll-like receptor (TLR) 2, TLR4 and MyD88, in exacerbation of allergen-induced lung eosinophilia caused by urban PM2.5 was investigated. TLR2-, TLR4-, MyD88-deficient and WT BALB/c mice were intratracheally challenged with PM2.5 +/− ovalbumin (OVA) four times at 2-week intervals. PM2.5 increased neutrophil numbers and KC in bronchoalveolar lavage fluid and caused slight peribronchiolar inflammation in WT mice. However, these changes were attenuated, but not completely suppressed in gene-deficient mice, especially in MyD88−/− mice. In WT mice, PM2.5 + OVA exacerbated OVA-related lung eosinophilia. This exacerbation includes increase of IL-5, IL-13, eotaxin and MCP-3; infiltration of eosinophils into the airway submucosa; proliferation of goblet cells in the airway epithelium; and the production of antigen-specific IgE and IgG1 in serum. All these effects were stronger in TLR2−/− mice than in TLR4−/− mice. In MyD88−/− mice, this pro-inflammatory mediator-inducing ability was considerably weak and lung pathology was negligible. These results suggest that urban PM2.5 may exacerbate allergic inflammation in the murine lung via a TLR2/TLR4/MyD88-signaling pathway. PM2.5-bound trace microbial elements, such as lipopolysaccharide may be a strong candidate for exacerbation of murine lung eosinophilia.
Temperature-Controlled Direct Imprinting of Ag Ionic Ink: Flexible Metal Grid Transparent Conductors with Enhanced Electromechanical Durability
Authors: Oh Yong Suk , Choi Hyesun , Lee Jaeho , Lee Hyunwoo , Choi Dong Yun , Lee Sung-Uk , Yun Kyeong-Soo , Yoo Seunghyup , Kim Taek-Soo , Park Inkyu , Sung Hyung Jin
Published: September 2017

Next-generation transparent conductors (TCs) require excellent electromechanical durability under mechanical deformations as well as high electrical conductivity and transparency. Here we introduce a method for the fabrication of highly conductive, low-porosity, flexible metal grid TCs via temperature-controlled direct imprinting (TCDI) of Ag ionic ink. The TCDI technique based on two-step heating is capable of not only stably capturing the Ag ionic ink, but also reducing the porosity of thermally decomposed Ag nanoparticle structures by eliminating large amounts of organic complexes. The porosity reduction of metal grid TCs on a glass substrate leads to a significant decrease of the sheet resistance from 21.5 to 5.5 Ω sq−1 with an optical transmittance of 91% at λ = 550 nm. The low-porosity metal grid TCs are effectively embedded to uniform, thin and transparent polymer films with negligible resistance changes from the glass substrate having strong interfacial fracture energy (~8.2 J m−2). Finally, as the porosity decreases, the flexible metal grid TCs show a significantly enhanced electromechanical durability under bending stresses. Organic light‐emitting diodes based on the flexible metal grid TCs as anode electrodes are demonstrated.
Formaldehyde Hemiacetal Sampling, Recovery, and Quantification from Electronic Cigarette Aerosols
Authors: Salamanca James C. , Munhenzva Ian , Escobedo Jorge O. , Jensen R. Paul , Shaw Angela , Campbell Robert , Luo Wentai , Peyton David H. , Strongin Robert M.
Published: September 2017

The electronic cigarette solvents propylene glycol and glycerol are known to produce toxic byproducts such as formaldehyde, acetaldehyde and acrolein. However, the aerosol toxin yield depends upon a variety of chemical and physical variables. The formaldehyde hemiacetals derived from these solvents were reported as major electronic cigarette aerosol components by us in 2015. In the study described herein, the formaldehyde hemiacetals were found at higher levels than those of free formaldehyde via an orthogonal sample collection protocol. In addition, the common aldehyde collection methods for electronic cigarettes, such as impingers and sorbent tubes containing DNPH, significantly underestimate the levels of formaldehyde. The reason for this is that formaldehyde hemiacetals follow other reaction pathways, such as the formation of a less reactive full cyclic acetal catalyzed by the acidity of the DNPH solution and the silica. We found that formaldehyde hemiacetals are a considerable fraction of the total formaldehyde produced in electronic cigarette that cannot be determined accurately by DNPH derivatization methods. Although the health effects of the hemiacetals are not yet known, they warrant further investigation.
Melt fluxing to elevate the forming ability of Al-based bulk metallic glasses
Authors: Yang B. J. , Lu W. Y. , Zhang J. L. , Wang J. Q. , Ma E.
Published: September 2017

Salt-fluxing treatment is an effective technique to improve the glass-forming ability (GFA) of bulk metallic glass (BMG)-forming melts, as demonstrated before in Pd- and Fe-based systems. However, it has been challenging to develop similar fluxing protocol for more reactive melts, such as Al-rich BMG-forming systems. Here we design new fluxing agents, from a thermodynamics perspective that takes into account combined effects of physical absorption and chemical absorption (reaction) between the fluxing agents and oxide inclusions. MgCl2-CaCl2 composite salts were selected, and their fluxing effects were systematically studied on an Al86Ni6.75Co2.25Y3.25La1.75 alloy, the best BMG-forming composition reported thus far for Al-rich alloy systems. The oxygen content was found to continuously decrease in the master alloy with increasing cycles of salt-fluxing treatment, with chlorate products on the surface suggesting concurrent physical absorption and chemical reaction. The fluxing treatment developed has enabled a record critical size (diameter) of 2.5 mm for Al-based BMGs. Our finding is thus an advance in developing highly desirable Al-based BMGs, and also provides guidance for designing processing protocol to produce larger-sized BMGs in other reactive systems.
Enhancing elevated temperature strength of copper containing aluminium alloys by forming L12 Al3Zr precipitates and nucleating θ″ precipitates on them
Authors: Kumar Makineni Surendra , Sugathan Sandeep , Meher Subhashish , Banerjee Rajarshi , Bhattacharya Saswata , Kumar Subodh , Chattopadhyay Kamanio
Published: September 2017

Strengthening by precipitation of second phase is the guiding principle for the development of a host of high strength structural alloys, in particular, aluminium alloys for transportation sector. Higher efficiency and lower emission demands use of alloys at higher operating temperatures (200 °C–250 °C) and stresses, especially in applications for engine parts. Unfortunately, most of the precipitation hardened aluminium alloys that are currently available can withstand maximum temperatures ranging from 150–200 °C. This limit is set by the onset of the rapid coarsening of the precipitates and consequent loss of mechanical properties. In this communication, we present a new approach in designing an Al-based alloy through solid state precipitation route that provides a synergistic coupling of two different types of precipitates that has enabled us to develop coarsening resistant high-temperature alloys that are stable in the temperature range of 250–300 °C with strength in excess of 260 MPa at 250 °C.
New Insight into the Concept of Carbonization Degree in Synthesis of Carbon Dots to Achieve Facile Smartphone Based Sensing Platform
Authors: Bagheri Zeinab , Ehtesabi Hamide , Rahmandoust Moones , Ahadian Mohammad Mahdi , Hallaji Zahra , Eskandari Farzaneh , Jokar Effat
Published: September 2017

Direct pyrolysis of citric acid (CA) has been proved to be a facile bottom–up technique for making pristine carbon dots (CD) with homogenous size distribution. However, limited reports are available on systematic optimization of carbonization degree. In this investigation, pyrolysis temperatures between 160 °C and 220 °C were studied, based on CA thermal decomposition path, using various heating durations. The effect of the formation of more carbonized carbon particles (MCCPs), as the major byproduct of this method, on photoluminescence properties of CDs was also considered. The NaOH amount that neutralizes the solution and the effect of dilution on the emission intensity, were introduced as simple and accessible factors for monitoring carbonization degree, and an estimate of MCCP/CD ratio, respectively. The results show that the CDs fabricated at 160 °C, 50 minutes attain almost twice higher quantum yield (QY) of 29% than highest QY reported based on pyrolysis of CA. The so–prepared CDs can be employed as excellent candidates for turn–off sensing. As a proof of concept, detection limit of 50 nM for Hg2+ was achieved using a facile and inexpensive smartphone set–up that is able to quantify and compare fluorescent intensity in several samples simultaneously.
Metabolomics differences between silkworms (Bombyx mori) reared on fresh mulberry (Morus) leaves or artificial diets
Authors: Dong Hui-Ling , Zhang Sheng-Xiang , Tao Hui , Chen Zhuo-Hua , Li Xue , Qiu Jian-Feng , Cui Wen-Zhao , Sima Yang-Hu , Cui Wei-Zheng , Xu Shi-Qing
Published: September 2017

Silkworms (Bombyx mori) reared on artificial diets have great potential applications in sericulture. However, the mechanisms underlying the enhancement of metabolic utilization by altering silkworm nutrition are unclear. The aim of this study was to investigate the mechanisms responsible for the poor development and low silk protein synthesis efficiency of silkworms fed artificial diets. After multi-generational selection of the ingestive behavior of silkworms to artificial diets, we obtained two strains, one of which developed well and another in which almost all its larvae starved to death on the artificial diets. Subsequently, we analyzed the metabolomics of larval hemolymph by gas chromatography/liquid chromatography–mass spectrometry, and the results showed that vitamins were in critically short supply, whereas the nitrogen metabolic end product of urea and uric acid were enriched substantially, in the hemolymph of the silkworms reared on the artificial diets. Meanwhile, amino acid metabolic disorders, as well as downregulation of carbohydrate metabolism, energy metabolism, and lipid metabolism, co-occurred. Furthermore, 10 male-dominant metabolites and 27 diet-related metabolites that differed between male and female silkworms were identified. These findings provide important insights into the regulation of silkworm metabolism and silk protein synthesis when silkworms adapt to an artificial diet.
Complementary ACSL isoforms contribute to a non-Warburg advantageous energetic status characterizing invasive colon cancer cells
Authors: Sánchez-Martínez Ruth , Cruz-Gil Silvia , García-Álvarez María Soledad , Reglero Guillermo , Ramírez de Molina Ana
Published: September 2017

Metabolic reprogramming is one of cancer hallmarks. Here, we focus on functional differences and individual contribution of acyl coA synthetases (ACSL) isoforms to the previously described ACSL/stearoyl-CoA desaturase (ACSL1/ACSL4/SCD) metabolic network causing invasion and poor prognosis in colorectal cancer (CRC). ACSL4 fuels proliferation and migration accompanied by a more glycolytic phenotype. Conversely, ACSL1 stimulates invasion displaying a lower basal respiratory rate. Acylcarnitines elevation, polyunsaturated fatty acids (PUFA) lower levels, and monounsaturated fatty acids (MUFA) upregulation characterize the individual overexpression of ACSL1, ACSL4 and SCD, respectively. However, the three enzymes simultaneous overexpression results in upregulated phospholipids and urea cycle derived metabolites. Thus, the metabolic effects caused by the network are far from being caused by the individual contributions of each enzyme. Furthermore, ACSL/SCD network produces more energetically efficient cells with lower basal respiration levels and upregulated creatine pathway. These features characterize other invasive CRC cells, thus, ACSL/SCD network exemplifies specific metabolic adaptations for invasive cancer cells.
Persistence of a stage-structured food-web
Authors: Mougi Akihiko
Published: September 2017

Contrary to a theoretical prediction, natural communities comprise many interacting species, thereby developing complex ecosystems. Earlier theoretical studies assumed that each component species within an ecological network has a simple life history, despite the fact that the interaction partners of many species, such as their predators and resources, change during the developmental stages. This poses an open question on the effect of life history complexity on the dynamics of communities. Here using a food web model, I showed that species with a stage-structured life cycle greatly changes the relationship between community complexity and persistence. Without stage-structured species, an increase in species diversity and interaction links decreases the community persistence, whereas in the presence of stage-structured species, community complexity can increase the community persistence. Therefore, life history complexity may be a key element of biodiversity that is self-maintaining.
A Novel Role For Nanog As An Early Cancer Risk Marker In Patients With Laryngeal Precancerous Lesions
Authors: Rodrigo Juan P. , Villaronga M. Ángeles , Menéndez Sofía T. , Hermida-Prado Francisco , Quer Miquel , Vilaseca Isabel , Allonca Eva , Pedregal Mallo Daniel , Astudillo Aurora , García-Pedrero Juana M.
Published: September 2017

NANOG is a master regulator of embryonic stem cell pluripotency, found to be frequently aberrantly expressed in a variety of cancers, including laryngeal carcinomas. This study investigates for the first time the role of NANOG expression in early stages of laryngeal tumourigenesis and its potential utility as cancer risk marker. NANOG protein expression was evaluated by immunohistochemistry using two large independent cohorts of patients with laryngeal precancerous lesions, and correlated with clinicopathological parameters and laryngeal cancer risk. NANOG expression was detected by immunohistochemistry in 49 (60%) of 82 laryngeal dysplasias, whereas expression was negligible in patient-matched normal epithelia. Strong NANOG expression was found in 22 (27%) lesions and was established as cut-off point, showing the most robust association with laryngeal cancer risk (P = 0.003) superior to the histological classification (P = 0.320) the current gold standard in the clinical practice. Similar trends were obtained using a multicenter validation cohort of 86 patients with laryngeal dysplasia. Our findings uncover a novel role for NANOG expression in laryngeal tumourigenesis, and its unprecedented application as biomarker for cancer risk assessment.
If these data could talk
Authors: Pasquier Thomas , Lau Matthew K. , Trisovic Ana , Boose Emery R. , Couturier Ben , Crosas Mercè , Ellison Aaron M. , Gibson Valerie , Jones Chris R. , Seltzer Margo
Published: September 2017

In the last few decades, data-driven methods have come to dominate many fields of scientific inquiry. Open data and open-source software have enabled the rapid implementation of novel methods to manage and analyze the growing flood of data. However, it has become apparent that many scientific fields exhibit distressingly low rates of reproducibility. Although there are many dimensions to this issue, we believe that there is a lack of formalism used when describing end-to-end published results, from the data source to the analysis to the final published results. Even when authors do their best to make their research and data accessible, this lack of formalism reduces the clarity and efficiency of reporting, which contributes to issues of reproducibility. Data provenance aids both reproducibility through systematic and formal records of the relationships among data sources, processes, datasets, publications and researchers.
Correction: Corrigendum: A 4-study replication of the moderating effects of greed on socioeconomic status and unethical behaviour
Authors: Balakrishnan Anjana , Palma Paolo A. , Patenaude Joshua , Campbell Lorne
Published: September 2017

Scientific Data 4:160120 doi: 10.1038/sdata.2016.120 (2017); Published 31 January 2017; Updated 5 September 2017. It was brought to the authors' attention that there was an error in the SPSS syntax for the regression models analyzing the main effects. We had recoded the SES variable so that higher numbers corresponded to a higher SES.
Nectin-3 modulates the structural plasticity of dentate granule cells and long-term memory
Authors: Wang X-X , Li J-T , Xie X-M , Gu Y , Si T-M , Schmidt M V , Wang X-D
Published: September 2017

Nectin-3, a cell adhesion molecule enriched in hippocampal neurons, has been implicated in stress-related cognitive disorders. Nectin-3 is expressed by granule cells in the dentate gyrus (DG), but it remains unclear whether nectin-3 in DG modulates the structural plasticity of dentate granule cells and hippocampus-dependent memory. In this study, we found that DG nectin-3 expression levels were developmentally regulated and reduced by early postnatal stress exposure in adult mice. Most importantly, knockdown of nectin-3 levels in all DG neuron populations by adeno-associated virus (AAV) mimicked the cognitive effects of early-life stress, and impaired long-term spatial memory and temporal order memory. Moreover, AAV-mediated DG nectin-3 knockdown increased the density of doublecortin-immunoreactive differentiating cells under proliferation and calretinin-immunoreactive immature neurons, but markedly decreased calbindin immunoreactivity, indicating that nectin-3 modulates the differentiation and maturation of adult-born DG granule cells. Using retrovirus to target newly generated DG neurons, we found that selective nectin-3 knockdown in new DG neurons also impaired long-term spatial memory. In addition, suppressing nectin-3 expression in new DG neurons evoked a reduction of dendritic spines, especially thin spines. Our data indicate that nectin-3 expressed in DG neurons may modulate adult neurogenesis, dendritic spine plasticity and the cognitive effects of early-life stress.
Vertically standing nanoporous Al–Ag zig-zag silver nanorod arrays for highly active SERS substrates
Authors: Rajput Ankita , Kumar Samir , Singh Jitendra P.
Published: September 2017

We have demonstrated a simple de-alloying method to create nanogaps in a vertically standing zigzag AgNR arrays which act as SERS active hot spots for better SERS sensitivity.
Ab initio characterization and experimental validation on the roles of oxygen-containing groups in graphene based formaldehyde sensors
Authors: Duan Liangping , Bo Zheng , Chen Xia , Qi Hualei , Yan Jianhua , Cen Kefa
Published: September 2017

The roles of oxygen-containing groups in formaldehyde sensing were investigated by ab initio characterization, in conjunction with experimental validation.
Enhanced anodic electrochemiluminescence of CdTe quantum dots based on electrocatalytic oxidation of a co-reactant by dendrimer-encapsulated Pt nanoparticles and its application for sandwiched immunoassays
Authors: Ren Lu-Lu , Dong Hao , Han Ting-Ting , Chen Yun , Ding Shou-Nian
Published: September 2017

A first immunosensor using Fe3O4@SiO2-Pt DENs for carrier separation and signal amplification in the CdTe QD-TPrA anodic ECL system.
A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T
Authors: Song Keum-Soo , Nimse Satish Balasaheb , Sonawane Mukesh Digambar , Lin Yahui , Zhou Zhou , Kim Taisun
Published: September 2017

A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL−1is described.
Nickel-catalyzed ring-opening of α-hydroxycyclobutenones with a remarkable ligand effect
Authors: Gao Songsong , Hu Xiangdong
Published: September 2017

A Ni-catalyzed ring-opening of α-hydroxycyclobutenones is reported herein.
Utilization of the dye N-methyl-6-oxyquinolone as an optical acidometer in molecular solvents and protic ionic liquids
Authors: Schmode Stella , Ludwig Ralf
Published: September 2017

We developed a procedure to use the highly sensitive dyeN-methyl-6-oxyquinolone as an acidity probe in molecular solvents and protic ionic liquids.
Microporous organic polymers involving thiadiazolopyridine for high and selective uptake of greenhouse gases at low pressure
Authors: Waseem Hussain MD. , Bandyopadhyay Sujoy , Patra Abhijit
Published: September 2017

Thiadiazolopyridine-based microporous organic polymers were shown to exhibit a remarkably high uptake of CO2 of 5.8 mmol g−1 at 273 K and 1 bar.
Dynamic protein self-assembly driven by host–guest chemistry and the folding–unfolding feature of a mutually exclusive protein
Authors: Wang Ruidi , Qiao Shanpeng , Zhao Linlu , Hou Chunxi , Li Xiumei , Liu Yao , Luo Quan , Xu Jiayun , Li Hongbin , Liu Junqiu
Published: September 2017

Taking advantage of cucurbit[8]uril-based supramolecular interactions, we constructed a novel redox-responsive assembly which was controllable to transform the morphology.
Two solvent-induced porous hydrogen-bonded organic frameworks: solvent effects on structures and functionalities
Authors: Wang Hailong , Bao Zongbi , Wu Hui , Lin Rui-Biao , Zhou Wei , Hu Tong-Liang , Li Bin , Zhao John Cong-Gui , Chen Banglin
Published: September 2017

The synthesis, crystal structures, gas sorption behaviours and fluorescence sensing applications of two HOFs have been systematically investigated to elucidate the solvent effects on their structures and functionalities.
An approach to spirooxindoles via palladium-catalyzed remote C–H activation and dual alkylation with CH2Br2
Authors: Shao Changdong , Wu Zhuo , Ji Xiaoming , Zhou Bo , Zhang Yanghui
Published: September 2017

An approach for the synthesis of spirooxindoles has been developed via palladium-catalyzed remote C–H functionalization with CH2Br2.
Recurrent supramolecular scenarios within complex 3-D hydrogen bond networks derived from organic ammonium salts of (4-amino-1-hydroxybutylidine)-1,1-bisphosphonic acid
Authors: Deacon G. B. , Forsyth C. M. , Greenhill N. B. , Junk P. C.
Published: September 2017

Reactions of (4-amino-1-hydroxybutylidine)-1,1-bisphosphonic acid (LH5) with organic amines or diamines in aqueous solution yields crystalline compounds of the general formula [(RN)H]2[LH3] or [(R'N)H2][LH3].
Proteins at air–water and oil–water interfaces in an all-atom model
Authors: Zhao Yani , Cieplak Marek
Published: September 2017

Proteins with different hydrophobicities are studied at the air–water and oil–water interfaces. The all-atom simulating results are consistent with the coarse-grained interfacial model. Proteins are found to be coupled stronger but diffused slower at the oil–water interface than the air–water one.
Probing RbBr solvation in freestanding sub-2 nm water clusters
Authors: Hautala Lauri , Jänkälä Kari , Mikkelä Mikko-Heikki , Turunen Paavo , Prisle Nønne L. , Patanen Minna , Tchaplyguine Maxim , Huttula Marko
Published: September 2017

Core level photoelectron spectra of freestanding sub-2 nm RbBr-water clusters reveals increased ion pairing beyond 2 mol kg−1 concentration.
Examination of molecular packing in orthogonal smectic liquid crystal phases: a guide for molecular design of functional smectic phases
Authors: Yamamura Yasuhisa , Murakoshi Takahito , Hishida Mafumi , Saito Kazuya
Published: September 2017

Two modes of packing of molten alkyl chains in smectic liquid crystals are revealed through analyzing the chain-length dependence of layer spacings.
Circular torsion induced fan-blade shaped wrinkling in two-dimensional nano-rings
Authors: Bao Hongwei , Huang Yuhong , Yang Zhi , Miao Yaping , Sun Yunjin , Bai Yu , Xu Kewei , Ma Fei
Published: September 2017

Fan-blade shaped wrinkling with a strain vortex is induced in 2D nano-rings to enhance the pseudomagnetic fields and optical funnel effect.
Fast and accurate prediction of proton affinities: revisiting the extended Koopmans' theorem for protons
Authors: Pedraza-González Laura , Charry Jorge , Quintero William , Alí-Torres Jorge , Reyes Andrés
Published: September 2017

In this work we propose schemes based on the extended Koopmans' theorem for quantum nuclei (eKT), in the framework of the any particle molecular orbital approach (APMO/KT), for the quantitative prediction of gas phase proton affinities (PAs).
Gas adsorption capacity in an all carbon nanomaterial composed of carbon nanohorns and vertically aligned carbon nanotubes
Authors: Puthusseri Divya , Babu Deepu J. , Okeil Sherif , Schneider Jörg J.
Published: September 2017

Carbon composites composed of arrays of vertically aligned carbon nanotubes and spherically aggregated carbon nanohorns show an enhanced CO2 adsorption capacity in the high pressure regime.
Revealing the correlation between charge carrier recombination and extraction in an organic solar cell under varying illumination intensity
Authors: Sharma Abhishek , Chauhan Mihirsinh , Bharti Vishal , Kumar Manoj , Chand Suresh , Tripathi Brijesh , Tiwari J. P.
Published: September 2017

Herein, a theoretical model has been outlined in order to study the variation of different device parameters of an organic solar cell with various illumination levels.
An enhanced nonpolarity effect of silica-supported perfluoroalkyl sulfonylimide on catalytic fructose dehydration
Authors: Zhang Hai-Juan , Cheng Yuan , Yuan Hong , Wang Yun , Ma Zhong-Hua
Published: September 2017

–SO2NHSO2C4F9/CF3-functionalized silica was prepared.
Single-ion magnetism in seven-coordinate YbIII complexes with distorted D5h coordination geometry
Authors: Wu Dong-Qing , Shao Dong , Wei Xiao-Qin , Shen Fu-Xing , Shi Le , Zhang Yi-Quan , Wang Xin-Yi
Published: September 2017

Two seven-coordinate compounds of pentagonal bipyramidal YbIII centers exhibiting field-induced slow magnetic relaxation were characterized structurally and magnetically.
Mg doped Li2FeSiO4/C nanocomposites synthesized by the solvothermal method for lithium ion batteries
Authors: Kumar Ajay , Jayakumar O. D. , Jagannath Jagannath , Bashiri Parisa , Nazri G. A. , Naik Vaman. M. , Naik Ratna
Published: September 2017

Despite having the same carbon content, Li2Fe0.99Mg0.01SiO4/C delivered the highest initial discharge capacity and also exhibited the best rate capability and cycle stability.
Cooperative water oxidation catalysis in a series of trinuclear metallosupramolecular ruthenium macrocycles
Authors: Kunz Valentin , Lindner Joachim O. , Schulze Marcus , Röhr Merle I. S. , Schmidt David , Mitrić Roland , Würthner Frank
Published: September 2017

Well-defined hydrogen-bonded water networks inside differently sized ruthenium macrocycles facilitate cooperative proton-coupled electron transfers, and accelerate the water oxidation catalysis.
Comprehensive evaluation of the cytotoxicity of CdSe/ZnS quantum dots in Phanerochaete chrysosporium by cellular uptake and oxidative stress
Authors: Hu Liang , Wan Jia , Zeng Guangming , Chen Anwei , Chen Guiqiu , Huang Zhenzhen , He Kai , Cheng Min , Zhou Chengyun , Xiong Weiping , Lai Cui , Xu Piao
Published: September 2017

The cellular uptake of QDs and the induced physiological responses in an organism are investigated.
Screening of potential anti-adipogenic effects of phenolic compounds showing different chemical structure in 3T3-L1 preadipocytes
Authors: Mosqueda-Solís Andrea , Lasa Arrate , Gómez-Zorita Saioa , Eseberri Itziar , Picó Catalina , Portillo María P.
Published: September 2017

This study was designed to analyze the anti-adipogenic effect of fifteen phenolic compounds from various chemical groups in 3T3-L1 pre-adipocytes.
Emulsion electro-oxidation of kraft lignin
Authors: Di Marino Davide , Aniko Vadim , Stocco Antonio , Kriescher Stefanie , Wessling Matthias
Published: September 2017

Products from lignin electro-oxidation are in situ extracted by MIBK in emulsion phase.
Catalytic fast pyrolysis of biomass: superior selectivity of hierarchical zeolites to aromatics
Authors: Jia L. Y. , Raad M. , Hamieh S. , Toufaily J. , Hamieh T. , Bettahar M. M. , Mauviel G. , Tarrighi M. , Pinard L. , Dufour A.
Published: September 2017

Mesopores are “highways” for mass transfer inside zeolite crystals and enhance the formation of mono-aromatic hydrocarbons from biomass pyrolysis.
Detection of membrane-bound and soluble antigens by magnetic levitation
Authors: Andersen Mikkel Schou , Howard Emily , Lu Shulin , Richard Matthew , Gregory Mark , Ogembo Gordon , Mazor Ofer , Gorelik Pavel , Shapiro Nathan I. , Sharda Anish V. , Ghiran Ionita
Published: September 2017

Magnetic levitation is a technique for measuring the density and the magnetic properties of objects suspended in a paramagnetic field.
Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics
Authors: Valentin Thomas M. , Leggett Susan E. , Chen Po-Yen , Sodhi Jaskiranjeet K. , Stephens Lauren H. , McClintock Hayley D. , Sim Jea Yun , Wong Ian Y.
Published: September 2017

Light-based 3D printing of alginate hydrogels using ionic crosslinking enables reversible patterning with controlled degradation.
Investigation of a common gene expression signature in gastrointestinal cancers using systems biology approaches
Authors: Baghaei Kaveh , Hosseinkhan Nazanin , Asadzadeh Aghdaei Hamid , Zali M. R.
Published: September 2017

According to GLOBOCAN 2012, the incidence and the mortality rate of colorectal, stomach and liver cancers are the highest among the total gastrointestinal (GI) cancers.
Gold nanoparticles optical properties induced by water and an ionic liquid (bmimBF4) inside cationic reverse micelles
Authors: Blach Diana , Martínez O. Fernando
Published: September 2017

Polar solvent interactions at the interface are a key factor affecting the final properties of nanoparticles, including size, shape and crystallinity.
The application of a bee glue-modified sensor in daclatasvir dual effect detection
Authors: Azab Shereen M. , Fekry Amany M.
Published: September 2017

A simple and novel carbon paste sensor containing chemically mixed propolis (bee glue) and graphene oxide (GO) was prepared, then electrochemical deposition of silver nanoparticles (AgNPs) was performed to fabricate a selective and sensible electrochemical sensor to detect Daclatasvir (DAC).
A cool and high salt-tolerant ionic liquid matrix for preferential ionization of phosphopeptides by negative ion MALDI-MS
Authors: Ling Ling , Xiao Chunsheng , Jiang Liyan , Wang Sheng , Li Ying , Chen Xuesi , Guo Xinhua
Published: September 2017

An optimized ILM G3THAP/PA matrix significantly improved the detection of phosphopeptides by negative ion MALDI-MS compared with using 3-AQ/CHCA/ADP and DHB/PA matrices.
Synthesis and characterization of molybdic acid immobilized on modified magnetic nanoparticles as a new and recyclable catalyst for the synthesis of chromeno[4,3-b]chromenes
Authors: Khosravian Foroogh , Karami Bahador , Farahi Mahnaz
Published: September 2017

Synthesis and characterization of molybdic acid immobilized on modified magnetic nanoparticles as a new catalyst is reported.
Three-dimensional flower-like Mg(OH)2@MoS2 nanocomposite: fabrication, characterization and high-performance sensing properties for NOx at room temperature
Authors: Kang Jiameng , Ikram Muhammad , Zhao Yiming , Zhang Jiawei , Ur Rehman Afrasiab , Gong Lihong , Shi Keying
Published: September 2017

A three-dimensional (3D) flower-like hierarchical Mg(OH)2@MoS2 nanocomposite was fabricated using ordinary hydrothermal technology.
Bis(4-nitroanilines) in interactions through a π-conjugated bridge: conformational effects and potential molecular switches
Authors: Guerrero Tomás , Santillan Rosa , García-Ortega Héctor , Morales-Saavedra Omar G. , Farfán Norberto , Lacroix Pascal G.
Published: September 2017

DFT computation suggests that two dipolar chromophores in rotation around an organic bridge could be aligned by an electric field.
Redox active molybdophosphate produced by Cu3(PO4)2nanospheres for enhancing enzyme-free electrochemical immunoassay of C-reactive protein
Authors: Tan Xiaofeng , Zhang Lianhua , Deng Xiaobo , Miao Luyang , Li He , zheng Gengxiu
Published: September 2017

Redox-active molybdophosphate produced by Cu3(PO4)2nanospheres has been directly employed for signal amplification of an enzyme-free electrochemical immunosensor.
AFM combined to ATR-FTIR reveals Candida cell wall changes under caspofungin treatment
Authors: Quilès Fabienne , Accoceberry Isabelle , Couzigou Célia , Francius Grégory , Noël Thierry , El-Kirat-Chatel Sofiane
Published: September 2017

AFM was combined to vibrational spectroscopy to decipher morphological, mechanical and biochemical changes induced by caspofungin treatment onCandida.
Decoration of reduced graphene oxide by gold nanoparticles: an enhanced negative photoconductivity
Authors: Wang Qi , Tu Yudi , Ichii Takashi , Utsunomiya Toru , Sugimura Hiroyuki , Hao Lifeng , Wang Rongguo , He Xiaodong
Published: September 2017

rGO was spin-coated onto an AuNP-array to prepare the rGO/AuNP negative photoconductivity devices with high photoelectric responsivity.
Electrically and thermally conductive underwater acoustically absorptive graphene/rubber nanocomposites for multifunctional applications
Authors: Li Ying , Xu Fan , Lin Zaishan , Sun Xianxian , Peng Qingyu , Yuan Ye , Wang Shasha , Yang Zhiyu , He Xiaodong , Li Yibin
Published: September 2017

Graphene is ideal filler in nanocomposites due to its unique mechanical, electrical and thermal properties.
Synthesis of substituted oxazoles via Pd-catalyzed tandem oxidative cyclization
Authors: Zhang Wei , Yu Wenlong , Yan Qiangqiang , Liu Zhanxiang , Zhang Yuhong
Published: September 2017

An easy approach to access multisubstituted oxazoles via tandem oxidative cyclization is developed.
Highly efficient and versatile synthesis of α,α-difluoro-γ-lactams via aminodifluoroalkylation of alkenes
Authors: Chen Hongtai , Wang Xiaoyang , Guo Minjie , Zhao Wentao , Tang Xiangyang , Wang Guangwei
Published: September 2017

An efficient approach to α,α-difluoro-γ-lactam derivatives was developed using a copper/amine catalyst via a tandem radical cyclization pathway.
Enhanced optical confinement of dielectric nanoparticles by two-photon resonance transition
Authors: Kittiravechote Aungtinee , Usman Anwar , Masuhara Hiroshi , Liau Ian
Published: September 2017

Two-photon resonance enhances optical confinement of nanoparticles.
Modeling alveolar injury using microfluidic co-cultures for monitoring bleomycin-induced epithelial/fibroblastic cross-talk disorder
Authors: He Jiarui , Chen Weixing , Deng Shijie , Xie Lan , Feng Juan , Geng Jing , Jiang Dingyuan , Dai Huaping , Wang Chen
Published: September 2017

Epithelial/fibroblastic cross-talk is consider to lead to pulmonary fibrosis, but its pathogenesis remains unclear because no appropriate models allow to visualize the complex disease processes at the human lung epithelial–interstitial interface.
Orange phosphor Li2SrSiON2:Eu2+ for blue light chip based warm white LEDs
Authors: Mao Aijun , Zhao Zhengyan , Wang Yuhua
Published: September 2017

Crystal structure and luminescence properties of LSSON:Eu2+ have been investigated.