bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Gut Microbiota in Patients with Colorectal Disease Based on 16S rRNA Gene Amplicon and Shallow Metagenomic Sequencing
Authors: Liu Yuanfeng , Li Xiang , Yang Yudie , Liu Ye , Wang Shijun , Ji Boyang , Wei Yongjun
Published: July 2021

The gastrointestinal tract, the largest human microbial reservoir, is highly dynamic. The gut microbes play essential roles in causing colorectal diseases. In the present study, we explored potential keystone taxa during the development of colorectal diseases in central China. Fecal samples of some patients were collected and were allocated to the adenoma (Group A), colorectal cancer (Group C), and hemorrhoid (Group H) groups. The 16S rRNA amplicon and shallow metagenomic sequencing (SMS) strategies were used to recover the gut microbiota. Microbial diversities obtained from 16S rRNA amplicon and SMS data were similar. Group C had the highest diversity, although no significant difference in diversity was observed among the groups. The most dominant phyla in the gut microbiota of patients with colorectal diseases were Bacteroidetes, Firmicutes, and Proteobacteria, accounting for >95% of microbes in the samples. The most abundant genera in the samples wereBacteroides,Prevotella, andEscherichia/Shigella, and further species-level and network analyses identified certain potential keystone taxa in each group. Some of the dominant species, such asPrevotella copri,Bacteroides dorei, andBacteroides vulgatus, could be responsible for causing colorectal diseases. The SMS data recovered diverse antibiotic resistance genes of tetracycline, macrolide, and beta-lactam, which could be a result of antibiotic overuse. This study explored the gut microbiota of patients with three different types of colorectal diseases, and the microbial diversity results obtained from 16S rRNA amplicon sequencing and SMS data were found to be similar. However, the findings of this study are based on a limited sample size, which warrants further large-scale studies. The recovery of gut microbiota profiles in patients with colorectal diseases could be beneficial for future diagnosis and treatment with modulation of the gut microbiota. Moreover, SMS data can provide accurate species- and gene-level information, and it is economical. It can therefore be widely applied in future clinical metagenomic studies.
Morphological, Structural, and Functional Networks Highlight the Role of the Cortical-Subcortical Circuit in Individuals With Subjective Cognitive Decline
Authors: Xu Xiaowen , Wang Tao , Li Weikai , Li Hai , Xu Boyan , Zhang Min , Yue Ling , Wang Peijun , Xiao Shifu
Published: July 2021

Subjective cognitive decline (SCD) is considered the earliest stage of the clinical manifestations of the continuous progression of Alzheimer’s Disease (AD). Previous studies have suggested that multimodal brain networks play an important role in the early diagnosis and mechanisms underlying SCD. However, most of the previous studies focused on a single modality, and lacked correlation analysis between different modal biomarkers and brain regions. In order to further explore the specific characteristic of the multimodal brain networks in the stage of SCD, 22 individuals with SCD and 20 matched healthy controls (HCs) were recruited in the present study. We constructed the individual morphological, structural and functional brain networks based on 3D-T1 structural magnetic resonance imaging (sMRI), diffusion tensor imaging (DTI) and resting-state functional magnetic resonance imaging (rs-fMRI), respectively. A t-test was used to select the connections with significant difference, and a multi-kernel support vector machine (MK-SVM) was applied to combine the selected multimodal connections to distinguish SCD from HCs. Moreover, we further identified the consensus connections of brain networks as the most discriminative features to explore the pathological mechanisms and potential biomarkers associated with SCD. Our results shown that the combination of three modal connections using MK-SVM achieved the best classification performance, with an accuracy of 92.68%, sensitivity of 95.00%, and specificity of 90.48%. Furthermore, the consensus connections and hub nodes based on the morphological, structural, and functional networks identified in our study exhibited abnormal cortical-subcortical connections in individuals with SCD. In addition, the functional networks presented more discriminative connections and hubs in the cortical-subcortical regions, and were found to perform better in distinguishing SCD from HCs. Therefore, our findings highlight the role of the cortical-subcortical circuit in individuals with SCD from the perspective of a multimodal brain network, providing potential biomarkers for the diagnosis and prediction of the preclinical stage of AD.
Establishment of an Immune Cell Infiltration Score to Help Predict the Prognosis and Chemotherapy Responsiveness of Gastric Cancer Patients
Authors: Jiang Quan , Sun Jie , Chen Hao , Ding Chen , Tang Zhaoqing , Ruan Yuanyuan , Liu Fenglin , Sun Yihong
Published: July 2021

The immune microenvironment plays a critical role in tumor biology. The molecular profiles of immune components and related genes are of tremendous value for the study of primary resistance to immune checkpoint blockers (ICBs) for gastric cancer (GC) and serve as prognostic biomarkers to predict GC survival. Recent studies have revealed that tumor immune cell infiltration (ICI) is an indicator of the survival and responsiveness to chemotherapy in GC patients. Here, we describe the immune cell landscape based on the ESTIMATE and CIBERSORT algorithms to help separate GC into 3 ICI clusters using the unsupervised clustering method. Further in-depth analyses, such as differential expression gene (DEG) analysis and principal component analysis (PCA), help to establish an ICI scoring system. A low ICI score is characterized by an increased tumor mutation burden (TMB). The combination of the ICI score and TMB score better predicts the survival of GC patients. Analyses based on public and our own database revealed that the ICI scoring system could also help predict the survival and chemotherapy responsiveness of GC patients. The present study demonstrated that the ICI score may be an effective prognostic biomarker and predictive indicator for chemotherapy and immunotherapy.
Hydroethanolic Extract of A. officinarum Hance Ameliorates Hypertension and Causes Diuresis in Obesogenic Feed-Fed Rat Model
Authors: Javaid Farah , Mehmood Malik Hassan , Shaukat Bushra
Published: July 2021

Alpinia officinarumHance (Zingiberaceae) has been used widely in traditional Chinese and Ayurvedic medicines. Its folkloric uses include relieving stomach ache, treating cold, improving the circulatory system, and reducing swelling. Its effectiveness and mechanism of antihypertension in obesity-induced hypertensive rats have not been studied yet as per our knowledge. This study has been designed to provide evidence of underlying mechanisms to the medicinal use ofA. officinarumas a cardiotonic using an obesity-induced hypertension model in rats. Chronic administration ofA. officinarumcaused a marked reduction in the body weight gain and Lee index of rats compared to the obesogenic diet-fed rats. Its administration also caused attenuation in blood pressure (systolic, diastolic, and mean), serum total cholesterol, triglyceride, and leptin, while an increase in serum HDL and adiponectin levels was noticed. The catalase and superoxide dismutase enzymatic activities were found to be remarkable in the serum ofA. officinarum-treated animal groups.A. officinarumshowed mild to moderate diuretic, hepatoprotective, and reno-protective effects.The A. officinarum-treated group showed less mRNA expression of 3-hydroxy-3-methylglutaryl-CoA reductase while the mRNA expression of peroxisome proliferator-activated receptor and mRNA expression of cholesterol 7 alpha-hydroxylase were raised in comparison to the hypertensive group of rats evaluated by quantitative real-time polymerase chain reaction. These findings show thatA. officinarumpossesses antihypertensive and diuretic activities, thus providing a rationale to the medicinal use ofA. officinarumin cardiovascular ailments.
Multi-Pharmacology of Berberine in Atherosclerosis and Metabolic Diseases: Potential Contribution of Gut Microbiota
Authors: Yang Shengjie , Li Dan , Yu Zongliang , Li Yujuan , Wu Min
Published: July 2021

Atherosclerosis (AS), especially atherosclerotic cardiovascular diseases (ASCVDs), and metabolic diseases (such as diabetes, obesity, dyslipidemia, and nonalcoholic fatty liver disease) are major public health issues worldwide that seriously threaten human health. Exploring effective natural product-based drugs is a promising strategy for the treatment of AS and metabolic diseases. Berberine (BBR), an important isoquinoline alkaloid found in various medicinal plants, has been shown to have multiple pharmacological effects and therapeutic applications. In view of its low bioavailability, increasing evidence indicates that the gut microbiota may serve as a target for the multifunctional effects of BBR. Under the pathological conditions of AS and metabolic diseases, BBR improves intestinal barrier function and reduces inflammation induced by gut microbiota-derived lipopolysaccharide (LPS). Moreover, BBR reverses or induces structural and compositional alterations in the gut microbiota and regulates gut microbe-dependent metabolites as well as related downstream pathways; this improves glucose and lipid metabolism and energy homeostasis. These findings at least partly explain the effect of BBR on AS and metabolic diseases. In this review, we elaborate on the research progress of BBR and its mechanisms of action in the treatment of AS and metabolic diseases from the perspective of gut microbiota, to reveal the potential contribution of gut microbiota to the multifunctional biological effects of BBR.
DELLA-NAC Interactions Mediate GA Signaling to Promote Secondary Cell Wall Formation in Cotton Stem
Authors: Wang Yi , Yu Wanting , Ran Lingfang , Chen Zhong , Wang Chuannan , Dou Yang , Qin Yuanyuan , Suo Qingwei , Li Yaohua , Zeng Jianyan , Liang Aimin , Dai Yonglu , Wu Yiping , Ouyang Xufen , Xiao Yuehua
Published: July 2021

Gibberellins (GAs) promote secondary cell wall (SCW) development in plants, but the underlying molecular mechanism is still to be elucidated. Here, we employed a new system, the first internode of cotton, and the virus-induced gene silencing method to address this problem. We found that knocking down major DELLA genes via VIGS phenocopied GA treatment and significantly enhanced SCW formation in the xylem and phloem of cotton stems. Cotton DELLA proteins were found to interact with a wide range of SCW-related NAC proteins, and virus-induced gene silencing of these NAC genes inhibited SCW development with downregulated biosynthesis and deposition of lignin. The findings indicated a framework for the GA regulation of SCW formation; that is, the interactions between DELLA and NAC proteins mediated GA signaling to regulate SCW formation in cotton stems.
Toward Unifying Evolutionary Ecology and Genomics to Understand Positive Plant–Plant Interactions Within Wild Species
Authors: Subrahmaniam Harihar Jaishree , Roby Dominique , Roux Fabrice
Published: July 2021

In a local environment, plant networks include interactions among individuals of different species and among genotypes of the same species. While interspecific interactions are recognized as main drivers of plant community patterns, intraspecific interactions have recently gained attention in explaining plant community dynamics. However, an overview of intraspecific genotype-by-genotype interaction patterns within wild plant species is still missing. From the literature, we identified 91 experiments that were mainly designed to investigate the presence of positive interactions based on two contrasting hypotheses. Kin selection theory predicts partisan help given to a genealogical relative. The rationale behind this hypothesis relies on kin/non-kin recognition, with the positive outcome of kin cooperation substantiating it. On the other hand, the elbow-room hypothesis supports intraspecific niche partitioning leading to positive outcome when genetically distant genotypes interact. Positive diversity-productivity relationship rationalizes this hypothesis, notably with the outcome of overyielding. We found that both these hypotheses have been highly supported in experimental studies despite their opposite predictions between the extent of genetic relatedness among neighbors and the level of positive interactions. Interestingly, we identified a highly significant effect of breeding system, with a high proportion of selfing species associated with the presence of kin cooperation. Nonetheless, we identified several shortcomings regardless of the species considered, such as the lack of a reliable estimate of genetic relatedness among genotypes and ecological characterization of the natural habitats from which genotypes were collected, thereby impeding the identification of selective drivers of positive interactions. We therefore propose a framework combining evolutionary ecology and genomics to establish the eco-genomic landscape of positive GxG interactions in wild plant species.
The Effects of Short-Term Exposure to Low Temperatures During the Booting Stage on Starch Synthesis and Yields in Wheat Grain
Authors: Zhang Wenjing , Zhao Yan , Li Lingyu , Xu Xu , Yang Li , Luo Zheng , Wang Beibei , Ma Shangyu , Fan Yonghui , Huang Zhenglai
Published: July 2021

Low temperatures (LT) in spring can have a major impact on the yields of wheat in winter. Wheat varieties with different cold sensitivities (the cold-tolerant Yannong 19 variety and the cold-sensitive Yangmai 18 variety) were used to study the responses of the wheat grain starch synthesis and dry material accumulation to short-term LT during the booting stage. The effects of short-term LT on the activities of key wheat grain starch synthesis enzymes, starch content and grain dry-matter accumulation were determined by exposing the wheat to simulated LT of from −2 to 2°C. Short-term LT stress caused a decrease in the fullness of the wheat grains along with decreased activities of adenosine diphosphate glucose pyrophosphorylase (AGPase, EC2.7.7.27), soluble starch synthase (SSS, EC2.4.1.21), granule-bound starch synthase (GBSS, EC2.4.1.21), and starch branching enzyme (SBE, EC2.4.1.18) at different spike positions during the filling stage. The rate of grain starch accumulation and starch content decreased with decreasing temperatures. Also, the duration of grain filling increased, the mean and the maximum filling rates were reduced and the quality of the grain dry-matter decreased. The number of grains per spike and the thousand-grain weight of the mature grains also decreased. Our data showed that short-term LT stress at the booting stage caused a decrease in the activities of key starch synthesis enzymes at the grain-filling stage. These changes reduced the accumulation of starch, decreased the filling rate, and lowered the accumulation of grain dry matter to ultimately decrease grain yields.
Conserved and Unique Roles of Chaperone-Dependent E3 Ubiquitin Ligase CHIP in Plants
Authors: Zhang Yan , Xia Gengshou , Zhu Qianggen
Published: July 2021

Protein quality control (PQC) is essential for maintaining cellular homeostasis by reducing protein misfolding and aggregation. Major PQC mechanisms include protein refolding assisted by molecular chaperones and the degradation of misfolded and aggregated proteins using the proteasome and autophagy. A C-terminus of heat shock protein (Hsp) 70-interacting protein [carboxy-terminal Hsp70-interacting protein (CHIP)] is a chaperone-dependent and U-box-containing E3 ligase. CHIP is a key molecule in PQC by recognizing misfolded proteins through its interacting chaperones and targeting their degradation. CHIP also ubiquitinates native proteins and plays a regulatory role in other cellular processes, including signaling, development, DNA repair, immunity, and aging in metazoans. As a highly conserved ubiquitin ligase, plant CHIP plays an important role in response to a broad spectrum of biotic and abiotic stresses. CHIP protects chloroplasts by coordinating chloroplast PQC both outside and inside the important photosynthetic organelle of plant cells. CHIP also modulates the activity of protein phosphatase 2A (PP2A), a crucial component in a network of plant signaling, including abscisic acid (ABA) signaling. In this review, we discuss the structure, cofactors, activities, and biological function of CHIP with an emphasis on both its conserved and unique roles in PQC, stress responses, and signaling in plants.
Root Endophytes and Ginkgo biloba Are Likely to Share and Compensate Secondary Metabolic Processes, and Potentially Exchange Genetic Information by LTR-RTs
Authors: Zou Kai , Liu Xueduan , Hu Qi , Zhang Du , Fu Shaodong , Zhang Shuangfei , Huang Haonan , Lei Fangying , Zhang Guoqing , Miao Bo , Meng Delong , Jiang Luhua , Liu Hongwei , Yin Huaqun , Liang Yili
Published: July 2021

Ginkgo biloba is a pharmaceutical resource for terpenes and flavonoids. However, few insights discussed endophytes’ role in Ginkgo, and whether genetic exchange happens between Ginkgo and endophytes remains unclear. Herein, functional gene profiles and repetitive sequences were analyzed to focus on these issues. A total of 25 endophyte strains were isolated from the Ginkgo root and distributed in 16 genera of 6 phyla. Significant morphological diversities lead to the diversity in the COG functional classification. KEGG mapping revealed that endophytic bacteria and fungi potentially synthesize chalcone, while endophytic fungi might also promote flavonoid derivatization. Both bacteria and fungi may facilitate the lignin synthesis. Aspergillus sp. Gbtc_1 exhibited the feasibility of regulating alcohols to lignans. Although Ginkgo and the endophytes have not observed the critical levopimaradiene synthase in ginkgolides synthesis, the upstream pathways of terpenoid precursors are likely intact. The MVK genes in Ginkgo may have alternative non-homologous copies or be compensated by endophytes in long-term symbiosis. Cellulomonas sp. Gbtc_1 became the only bacteria to harbor both MEP and MVA pathways. Endophytes may perform the mutual transformation of IPP and DMAPP in the root. Ginkgo and bacteria may lead to the synthesis and derivatization of the carotenoid pathway. The isoquinoline alkaloid biosynthesis seemed lost in the Ginkgo root community, but L-dopa is more probably converted into dopamine as an essential signal-transduction substance. So, endophytes may participate in the secondary metabolism of the Ginkgo in a shared or complementary manner. Moreover, a few endophytic sequences predicted as Ty3/Gypsy and Ty1/Copia superfamilies exhibited extremely high similarity to those of Ginkgo. CDSs in such endophytic LTR-RT sequences were also highly homologous to one Ginkgo CDS. Therefore, LTR-RTs may be a rare unit flowing between the Ginkgo host and endophytes to exchange genetic information. Collectively, this research effectively expanded the insight on the symbiotic relationship between the Ginkgo host and the endophytes in the root.
A Compendium of in vitro Germination Media for Pollen Research
Authors: Tushabe Donam , Rosbakh Sergey
Published: July 2021

The correct choice of in vitro pollen germination media (PGM) is crucial in basic and applied pollen research. However, the methodological gaps (e.g., strong focus of current research on model species and cultivated plants along with the lack of general rules for developing a PGM) makes experimenting with pollen difficult. We closed these gaps by compiling a compendium of optimized in vitro PGM recipes from more than 1800 articles published in English, German, and Russian from 1926 to 2019. The compendium includes 1572 PGM recipes successfully used to germinate pollen grains or produce pollen tubes in 816 species representing 412 genera and 114 families (both monocots and dicots). Among the 110 components recorded from the different PGM recipes, sucrose (89% of species), H3BO3 (77%), Ca2+ (59%), Mg2+ (44%), and K+ (39%) were the most commonly used PGM components. PGM pH was reported in 35% of all studies reviewed. Also, we identified some general rules for creating PGM for various groups of species differing in area of research (wild and cultivated species), phylogenetic relatedness (angiosperms vs. gymnosperms, dicots vs. monocots), pollen physiology (bi- and tri-cellular), biochemistry (starchy vs. starchless pollen grains), and stigma properties (dry vs. wet), and compared the component requirements. Sucrose, calcium, and magnesium concentrations were significantly different across most categories indicating that pollen sensitivity to sugar and mineral requirements in PGM is highly group-specific and should be accounted for when composing new PGM. This compendium is an important data resource on PGM and can facilitate future pollen research.
Review: Posed vs. Genuine Facial Emotion Recognition and Expression in Autism and Implications for Intervention
Authors: Webster Paula J. , Wang Shuo , Li Xin
Published: July 2021

Different styles of social interaction are one of the core characteristics of autism spectrum disorder (ASD). Social differences among individuals with ASD often include difficulty in discerning the emotions of neurotypical people based on their facial expressions. This review first covers the rich body of literature studying differences in facial emotion recognition (FER) in those with ASD, including behavioral studies and neurological findings. In particular, we highlight subtle emotion recognition and various factors related to inconsistent findings in behavioral studies of FER in ASD. Then, we discuss the dual problem of FER – namely facial emotion expression (FEE) or the production of facial expressions of emotion. Despite being less studied, social interaction involves both the ability to recognize emotions and to produce appropriate facial expressions. How others perceive facial expressions of emotion in those with ASD has remained an under-researched area. Finally, we propose a method for teaching FER [FER teaching hierarchy (FERTH)] based on recent research investigating FER in ASD, considering the use of posed vs. genuine emotions and static vs. dynamic stimuli. We also propose two possible teaching approaches: (1) a standard method of teaching progressively from simple drawings and cartoon characters to more complex audio-visual video clips of genuine human expressions of emotion with context clues or (2) teaching in a field of images that includes posed and genuine emotions to improve generalizability before progressing to more complex audio-visual stimuli. Lastly, we advocate for autism interventionists to use FER stimuli developed primarily for research purposes to facilitate the incorporation of well-controlled stimuli to teach FER and bridge the gap between intervention and research in this area.
Prevalence and Correlates of Social Stigma Toward Diabetes: Results From a Nationwide- Survey in Singapore
Authors: Subramaniam Mythily , Abdin Edimansyah , Bhuvaneswari S. , AshaRani P. V. , Devi Fiona , Roystonn Kumarasan , Wang Peizhi , Samari Ellaisha , Shafie Saleha , Vaingankar Janhavi Ajit , van Dam Rob M. , Lee Eng Sing , Sum Chee Fang , Chong Siow Ann
Published: July 2021

Aims: To examine the extent of social stigma toward diabetes among Singapore's multi-ethnic general population and determine whether this differs across socio-demographic sub-groups.

Methods: Data for this study came from a nationwide cross-sectional study. A diabetes stigma questionnaire comprising Social Distance Scale and Negative Attitudes and Stereotyping Scale was administered to those respondents who had not been diagnosed with diabetes. Exploratory factor analysis was conducted to determine the dimensionality of the instruments and validated using confirmatory factor analysis. Multiple linear regression analysis was conducted to examine associations between socio-demographic factors and measures of diabetes stigma.

Results: In all, 2,895 participants were recruited from the general population giving a response rate of 66.2%. Factor analyses found that a one-factor model resulted in an acceptable fit for both stigma scales, which measured social distance and negative attitudes and stereotyping, respectively. Multiple linear regression analyses identified Indian ethnicity (vs. Chinese), higher personal income (≥SGD2000 vs. < SGD 2000) and having close friends or family members who had been diagnosed with diabetes to be significantly associated with lower social distance scores while those aged 50–64 years and those with secondary and vocational education (vs. degree and above) were significantly associated with higher social distance scores. Those with a personal income of SG$2,000–3,999 and SGD $6,000 and above, and those with close friends or family members diagnosed with diabetes were significantly associated with lower negative attitudes and stereotyping scores. In contrast those aged 35 years and above, those with primary education and below, and those of Malay ethnicity were significantly associated with higher negative attitudes and stereotyping scores.

Conclusions: The study found a relatively low level of stigma toward diabetes in the general population of Singapore, although some stigmatizing beliefs emerged. While greater knowledge of diabetes could reduce stigma, anti-stigma messaging should be incorporated into the “War on Diabetes” programme in Singapore.
Attitude Toward Mathematics of Future Teachers: How Important Are Creativity and Cognitive Flexibility?
Authors: de-la-Peña Cristina , Fernádez-Cézar Raquel , Solano-Pinto Natalia
Published: July 2021

The attitude toward mathematics is shaped by cognitive components such as beliefs and cognitive processes. However, the importance of cognitive processes in attitude toward mathematics has not yet been researched. Therefore, this study aimed to identify the role of cognitive processes, creativity and cognitive flexibility, in the attitude toward mathematics of future teachers. For that purpose, 218 University students and preservice teachers, completed assignments on creativity and cognitive flexibility and a questionnaire on attitude toward mathematics. The results showed that the use of innovative details (a creativity subscale) rises the probability of exhibiting a positive attitude toward mathematics by 1.81. Besides, cognitive flexibility rises this probability by 2.32. The conclusion is that both, details and cognitive flexibility act as good predictors of a positive attitude toward mathematics. This has implications for educational practice in the planning of mathematics instruction in higher education, specifically, in future teachers.
International Child Abduction: The Complexities of Forensic Psychiatric Assessments Before the Hague Convention
Authors: Patel Mitesh , Baldeo Shawn , Swartz Pip , Glancy Graham
Published: July 2021

The Hague Convention is an international intergovernmental agreement that facilitates the return of abducted children to lawful parents across international borders. Children may not be returned if it can be established that the return would result in harm to the child. Forensic psychiatrists may be called upon to provide an expert opinion regarding the potential harm to come to a child, as well as various other psycholegal issues. We discuss interpretations and precedents regarding this law and the possible contributions of forensic psychiatrists. We also discuss two hybridized case examples involving international child abduction and proceedings before the Hague Convention. We will discuss issues that arose after psychiatric evaluations in each case.
Unveiling the Metabolic Profile of First-Episode Drug-Naïve Schizophrenia Patients: Baseline Characteristics of a Longitudinal Study Among Han Chinese
Authors: Zhang Qi , He Hui , Bai Xia , Jiang Liping , Chen Wei , Zeng Xiaoying , Li Yanjia , Teixeira Antonio L. , Dai Jing
Published: July 2021

Objective: Metabolic and other medical conditions are frequently comorbid with schizophrenia. As they might be the side-effects of antipsychotic treatment, studying first-episode drug-naïve schizophrenia (FDSZ) provides a unique opportunity to investigate a direct pathogenic link between metabolic changes and schizophrenia. Here, we presented the methods and baseline unique metabolic profile of FDSZ patients without medical comorbidities unveiling subthreshold indices of metabolic disturbances.

Method: Drug-naïve individuals diagnosed with schizophrenia but without any previous medical conditions were invited to participate in the study. Participants were submitted to structured psychiatric and cognitive assessments, laboratory and neuroimaging tests. Subjects will be followed after antipsychotic treatment at 6, 24 and 48 weeks.

Results: During an 8-month-period, out of 103 patients presenting with first episode psychosis, 67 subjects (43.3% men, 56.7% women) were enrolled in the study. They had a mean ± SD age of 32.1 ± 8.7 years, with a mean BMI of 21.1 kg/m2 and 11.3 ± 3.6 years of schooling. Less than 1/3 reported a family history of mental illness. Upon laboratory assessment, 10.4%, 7.5%, and 11.9% of patients were identified with hyperhomocysteinemia, hypertriglyceridemia and hyperprolactinemia, respectively, with percentages of women relatively higher than men except for hypertriglyceridemia.

Conclusions: First episode schizophrenia patients, especially women, present subclinical metabolic abnormalities, independent of antipsychotic treatment.
Effects of Urban Development Patterns on Municipal Water Shortage
Authors: Heidari Hadi , Arabi Mazdak , Warziniack Travis , Sharvelle Sybil
Published: July 2021

While urban areas are being threatened by water shortage due to climate change and rapid population growth, effects of urban development patterns on future municipal water shortage are rarely investigated. We address this aspect of urbanization by assessing the impacts of sprawl vs. high-density patterns on future changes in the sub-annual water shortage intensity-duration-frequency (IDF) relationships. The City of Fort Collins, Colorado, water supply system is chosen as a representative region that is rapidly developing over the last decades. The future water supply is estimated using the Soil and Water Assessment Tool (SWAT) driven with a hot-dry climate model from the statistically downscaled Coupled Model Intercomparison Project, phase 5 (CMIP5) projections. Future water demand is projected using the Integrated Urban Water Model (IUWM) under both sprawl and high-density development patterns. The demonstration study reveals that urban areas under the sprawl development pattern are likely to experience water shortage events with higher intensity, duration, and frequency compared to the high-density pattern. Characterizing impacts of urban development patterns on future water shortage conditions is required for sustainable water management and smart urban growth and can help urban planners and water managers to develop an adaptive path to meet future water demand and decrease the vulnerability of municipal water supply systems to shortage.
Motives for Dropout Among Former Junior Elite Caribbean Track and Field Athletes: A Qualitative Investigation
Authors: Thomas Candice E. , Chambers Timothy P. , Main Luana C. , Gastin Paul B.
Published: July 2021

Anecdotal reports within the Caribbean track and field fraternity have revealed that there is a high level of athlete dropout from competitive sport at the junior-elite level, and a poor transition to senior-elite status. Consequently, this qualitative investigation explored the key motives that may have contributed toward the unsuccessful transitions and ensuing dropout of Caribbean track and field athletes during the junior to senior transition period. Eleven former junior-elite track and field athletes (four males, seven females; Mage= 29, SD ± 4.2 years) from four English-speaking Caribbean islands participated in semi-structured interviews. Following an inductive and deductive thematic analysis, four higher order themes were identified: (1) “there's not enough support”; (2) “felt pressure to make sure I committed”; (3) “it's always competitive here”; and (4) “battle with the injuries.” For these former junior-elite Caribbean athletes, the decision on whether to continue within the sport was influenced by a combination of factors, although inadequate financial and organizational support had the most bearing on athletes' decision to drop out during the crucial transition years. Implications for consideration by key stakeholders and policymakers within the region are discussed.
The Role of Upper Body Biomechanics in Elite Racewalkers
Authors: Gravestock Helen J. , Tucker Catherine B. , Hanley Brian
Published: July 2021

The aim of this study was to analyze the link between the upper and lower body during racewalking. Fifteen male and 16 female racewalkers were recorded in a laboratory as they racewalked at speeds equivalent to their 20-km personal records [men: 1:23:12 (±2:45); women: 1:34:18 (±5:15)]; a single representative trial was chosen from each athlete for analysis and averaged data analyzed. Spatial variables (e.g., stride length) were normalized to stature and referred to as ratios. None of the peak upper body joint angles were associated with speed (p < 0.05) and there were no correlations between pelvic motion and speed, but a medium relationship was observed between peak pelvic external rotation (right pelvis rotated backwards) and stride length ratio (r = 0.37). Greater peak shoulder extension was associated with lower stride frequencies (r = −0.47) and longer swing times (r = 0.41), whereas peak elbow flexion had medium associations with flight time (r = −0.44). Latissimus dorsi was the most active muscle at toe-off during peak shoulder flexion; by contrast, pectoralis major increased in activity just before initial contact, concurrent with peak shoulder extension. Consistent but relatively low rectus abdominis and external oblique activation was present throughout the stride, but increased in preparation for initial contact during late swing. The movements of the pelvic girdle were important for optimizing spatiotemporal variables, showing that this exaggerated movement allows for greater stride lengths. Racewalkers should note however that a larger range of shoulder swing movements was found to be associated with lower stride frequency, and smaller elbow angles with increased flight time, which could be indicative of faster walking but can also lead to visible loss of contact. Coaches should remember that racewalking is an endurance event and development of resistance to fatigue might be more important than strength development.
Nepheline Syenite and Phonolite as Alternative Potassium Sources for Maize
Authors: Nogueira Thiago Assis Rodrigues , Miranda Bruno Gasparoti , Jalal Arshad , Lessa Luís Gustavo Frediani , Filho Marcelo Carvalho Minhoto Teixeira , Marcante Nericlenes Chaves , Abreu-Junior Cassio Hamilton , Jani Arun Dilipkumar , Capra Gian Franco , Moreira Adônis , Martins Éder de Souza
Published: July 2021

Some silicate rocks are a rich source of potassium (K), with the possibility for use in agriculture. The present study aimed to evaluate the agronomic efficiency index (AEI) of nepheline syenite (NS) and phonolite (PN) rocks in comparison with potassium chloride (KCl) as a K source in maize production. An experiment was conducted in a greenhouse in Ilha Solteira, São Paulo, Brazil. A maize hybrid was grown in 8 L pots filled with 6 kg of soil with a low K concentration and contrasting physical attributes (medium and sandy texture). A completely randomized design in a 3 × 6 factorial scheme was used, consisting of three K sources (NS, PN, and KCl) and six rates (0, 50, 100, 150, 200, and 400 mg kg−1) with four replications. All plants were harvested 45 days after emergence to evaluate biomass production, macronutrient (N, P, K, Ca, Mg, and S) concentration and uptake, stem diameter, and leaf chlorophyll index. After crop harvest, soil was collected for further chemical evaluation, which included organic matter (OM), pH, cation exchange capacity (CEC), H+Al, Al, sum of bases (SB), base saturation (BS), P, K, Ca, Mg, and S. In addition, AEI of NS and PN were also verified in relation to KCl. The application of NS and PN had a similar effect on soil chemical attributes (MO, pH, SB, CEC, and BS) as well as on the concentrations of K, Ca, Mg, and S, in both soils. The increase in NS and PN rates provided linear growth of shoot dry matter. Leaf macronutrient concentrations were similar for NS and PN compared to KCl. All three K sources (NS, PN, and KCl) increased K accumulation in maize plants. Maize treated with KCl had the largest AEI, followed by PN and NS. However, the results indicated similar AEI with both rocks as a K source for maize, especially with application of the highest K rates. This research demonstrated the efficiency of NS and PN as alternative K sources for maize.
Going Beyond Soil Conservation with the Use of Cover Crops in Mediterranean Sloping Olive Orchards
Authors: Arias-Giraldo Luis F. , Guzmán Gema , Montes-Borrego Miguel , Gramaje David , Gómez José A. , Landa Blanca B.
Published: July 2021

Among the agricultural practices promoted by the Common Agricultural Policy to increase soil functions, the use of cover crops is a recommended tool to improve the sustainability of Mediterranean woody crops such as olive orchards. However, there is a broad range of cover crop typologies in relation to its implementation, control and species composition. In that sense, the influence of different plant species on soil quality indicators in olive orchards remains unknown yet. This study describes the effects of four treatments based on the implementation of different ground covers (CC-GRA: sown cover crop with gramineous, CC-MIX: sown cover crop with a mixture of species and CC-NAT: cover crop with spontaneous vegetation) and conventional tillage (TILL) on soil erosion, soil physicochemical and biological properties after 8 years of cover crop establishment. Our results demonstrated that the presence of a temporary cover crop (CC), compared to a soil under tillage (TILL), can reduce soil losses and maintain good soil physicochemical properties and modify greatly the structure and diversity of soil bacterial communities and its functioning. The presence of a homogeneous CC of gramineous (Lolium rigidum or Lolilum multiflorum) (CC-GR) for 8 years increased the functional properties of the soil as compared to TILL; although the most relevant change was a modification on the bacterial community composition that was clearly different from the rest of treatments. On the other hand, the use of a mixture of plant species (CC-MIX) as a CC for only two years although did not modify greatly the structure and diversity of soil bacterial communities compared to the TILL soil, induced significant changes on the functional properties of the soil and reverted those properties to a level similar to that of an undisturbed soil that had maintained a natural cover of spontaneous vegetation for decades (CC-NAT).
Xenia and Deficit Nitrogen Influence the Iron and Zinc Concentration in the Grains of Hybrid Maize
Authors: Akhtar Sajjad , Labuschagne Maryke , Osthoff Gernot , Mashingaidze Kingston , Hossain Akbar
Published: July 2021

Xenia is the immediate effect of pollen on seed development after pollination. This study was conducted to investigate xenia’s effects on Fe and Zn concentration in self- and open-pollinated maize seeds. Eighteen maize hybrids derived from parents with varying concentrations of Fe and Zn were planted at Potchefstroom, Cedara and Vaalharts in South Africa for two seasons, 2017 and 2018. Open- and self-pollinated seeds were compared. Self-pollination of five selected healthy maize hybrids of uniform height from each plot was performed. Fe, Zn and phytic acid (Pa) analysis was conducted on self -and open-pollinated maize seeds, and the molar ratios of Fe and Zn to Pa were also calculated to assess the bioavailability of Fe and Zn. The ranges of Fe, Zn and Pa, and the molar ratios of Fe and Zn to Pa in self-pollinated maize seeds were 15.93–21.36 mg kg−1, 18.50–24.34 mg kg−1, 4.63–5.84 mg g−1, 21.97–31.22 and 23.53–30.16 under high N, and 12.76–19.29 mg kg−1, 16.78–23.50 mg kg−1, 5.05–6.48 mg g−1, 26.12–44.54 and 25.44–35.94 under low N conditions, respectively. The ranges of Fe, Zn and Pa, and the molar ratios of Fe and Zn to Pa in open-pollinated seeds were 14.34–19.12 mg kg−1, 17.27–23.27 mg kg−1, 4.96–5.89 mg g−1, 20.90–28.22 and 22.92–29.37 under high N, and 12.54–18.39 mg kg−1, 16.94–20.93 mg kg−1, 5.30–6.17 mg g−1, 27.90–38.75 and 27.95–36.34 under low N conditions, respectively. The bioavailability of Fe and Zn was high in self-pollinated seeds under high N conditions, while Fe was higher in self-pollinated seeds, and Zn was higher in open-pollinated seeds under low N conditions. Combined over seasons, there were no significant differences between the values of Fe and Zn in self- and open-pollinated seeds under both high N and low N conditions. This indicated that unknown males had no influence on Fe and Zn concentration in maize. Therefore, either self- or open-pollinated seeds can be used for Fe and Zn determination in maize crosses.
Animal Transmission of SARS-CoV-2 and the Welfare of Animals during the COVID-19 Pandemic
Authors: Ekstrand Kimberly , Flanagan Amanda J. , Lin Ilyan E. , Vejseli Brendon , Cole Allicyn , Lally Anna P. , Morris Robert L. , Morgan Kathleen N.
Published: July 2021

The accelerated pace of research into Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) necessitates periodic summaries of current research. The present paper reviews virus susceptibilities in species with frequent human contact, and factors that are best predictors of virus susceptibility. Species reviewed were those in contact with humans through entertainment, pet, or agricultural trades, and for whom reports (either anecdotal or published) exist regarding the SARS-CoV-2 virus and/or the resulting disease state COVID-19. Available literature was searched using an artificial intelligence (AI)-assisted engine, as well as via common databases, such as Web of Science and Medline. The present review focuses on susceptibility and transmissibility of SARS-CoV-2, and polymorphisms in transmembrane protease serine 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2) that contribute to species differences. Dogs and pigs appear to have low susceptibility, while ferrets, mink, some hamster species, cats, and nonhuman primates (particularly Old World species) have high susceptibility. Precautions may therefore be warranted in interactions with such species, and more selectivity practiced when choosing appropriate species to serve as models for research.
Determinants of Antimicrobial Resistance among the Different European Countries: More than Human and Animal Antimicrobial Consumption
Authors: Silva Ana C. , Nogueira Paulo Jorge , Paiva José-Artur
Published: July 2021

Although antimicrobial consumption is considered the main driver of antimicrobial resistance (AMR), other factors probably have a significant but less studied impact. The study’s goal was to assess AMR drivers across different European countries and quantify their possible contributions using the latest data available. Using the ESAC-Net (European Surveillance of Antimicrobial Consumption Network) database, the ESVAC (European Surveillance of Veterinary Antimicrobial Consumption) database and the OECD (Organisation for Economic Cooperation and Development) information, a dataset including 23 European countries was created. Associations between AMR and potential contributing factors were assessed using bivariate correlation and multiple linear regression models for multivariable analyses. Factors associated with the AMR rate among European countries were human ambulatory consumption of antibiotics and per capita expenditure on health, meaning that the higher human ambulatory consumption of antibiotics and the lower the per capita expenditure on health, the higher the AMR. Both variables together explain 74% of AMR variation. Private expenditure on health in terms of % GDP (Gross Domestic Profit) was positively related to a higher AMR rate. In conclusion, considering antibiotic consumption as the most important factor contributing to AMR may be a deviant focus, as resistance transmission may be paramount for AMR levels. Low per capita expenditure on health, probably a surrogate of worse healthcare conditions and a high level of resistance transmission, has a strong correlation with the AMR rate. Increasing public expenditure on healthcare, to strengthen infection control structures and processes interventions, seems relevant to tackle antimicrobial resistance at the European scale.
Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products
Authors: Kalová Alžběta , Gelbíčová Tereza , Overballe-Petersen Søren , Litrup Eva , Karpíšková Renáta
Published: July 2021

Aquaculture systems are widely recognised as hotspots for horizontal gene transfer, and the need for screening for bacteria carrying antimicrobial resistance genes in aquaculture systems is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and A. nosocomialis) resistant to colistin originating from retailed aquaculture products imported from Vietnam to the Czech Republic. The mcr-1.1 gene was found located on plasmid types IncHI2, IncI2, and IncX4, as well as on the rarely described plasmid types IncFIB-FIC and IncFIB(K), phage-like plasmid p0111, and on the chromosome of E. coli. One E. coli strain carried the mcr-3.5 gene on IncFII(pCoo) plasmid in addition to the mcr-1.1 gene located on IncHI2 plasmid. K. pneumoniae was found to carry the mcr-1.1 and mcr-8.2 genes on IncFIA(HI1) plasmid. The mcr-4.3 gene was found on similar untypeable plasmids of A. baumannii and A. nosocomialis strains, pointing to the possible interspecies transfer of plasmids carrying the mcr-4 gene. Our results highlight that some aquaculture products of Asian origin can represent an important source of variable plasmids carrying mcr genes. The results showed an involvement of phages in the incorporation of the mcr-1 gene into plasmids or the chromosome in E. coli strains from aquaculture. The detection of E. coli with the mcr-1 gene in the chromosome points to the risks associated with the stabilisation of the mcr genes in the bacterial chromosome.
A Multifunctional Nanoplatform Made of Gold Nanoparticles and Peptides Mimicking the Vascular Endothelial Growth Factor
Authors: Di Pietro Patrizia , Zimbone Stefania , Grasso Giulia , La Mendola Diego , Cossement Damien , Snyders Rony , Satriano Cristina
Published: July 2021

In this work, nanobiohybrids of plasmonic gold nanoparticles (AuNP, anti-angiogenic) and a peptide mimicking the vascular endothelial growth factor (VEGF, pro-angiogenic) were assembled and scrutinized in terms of physicochemical characterization, including optical properties, surface charge, surface chemical structure and morphology of the bioengineered metal nanoparticles, for their potential application as multifunctional theranostic (i.e., therapy + sensing) nanoplatform (AuNP/VEGF). Specifically, a peptide sequence encompassing the VEGF cellular receptor domain 73–101 (VEGF73–101) and its single point cysteine mutated were immobilized onto AuNP by physi- and chemi-sorption, respectively. The new hybrid systems were characterized by means of a multitechnique approach, including dynamic light scattering (DLS) analyses, zeta potential (ZP), spectroscopic (UV-Vis, FT-IR, XPS), spectrometric (TOF-SIMS) and microscopic (AFM, SEM) techniques. Proof-of-work cellular experiments in human umbilical vein endothelial cells (HUVEC) upon the treatment with AuNP/VEGF samples, demonstrated no toxicity up to 24 h (MTT assay) as well an effective internalization (laser confocal microscopy, LSM).