bluuebunny/crossref_metadata_2025 huggingface dataset:
Salivary cardiac-enriched FHL2-interacting protein is associated with higher diastolic-to-systolic-blood pressure ratio, sedentary time and center of pressure displacement in healthy 7-9 years old school-children
Authors: Vasileva Fidanka , Font-Lladó Raquel , Carreras-Badosa Gemma , Roman-Viñas Blanca , Cadellans-Arróniz Aïda , López-Bermejo Abel , Prats-Puig Anna
Published: January 2024

Introduction

Cardiac-enriched FHL2-interacting protein (CEFIP) is a recently identified protein, first found in the z-disc of striated muscles, and related to cardiovascular diseases. Our objectives are: 1) to quantify CEFIP in saliva in healthy 7-9 years old school-children; and 2) to assess the associations of salivary CEFIP concentration and blood pressure, physical (in)activity and physical fitness in these children.

Methods

A total of 72 children (7.6 ± 0.3 years) were included in the study, recruited in primary schools in Girona (Spain). A sandwich enzyme-linked immunosorbent assay was used (abx506878; Abbexa, United Kingdom) to quantify CEFIP in saliva. Anthropometric evaluation was performed [body mass, height and body mass index (BMI)]. Systolic and diastolic blood pressure were measured by means of an electronic oscillometer and the diastolic-to-systolic blood pressure ratio (D/S BP ratio) was calculated. Physical (in)activity [sedentary time and time spent in physical activity (PA)] were assessed by means of a triaxial Actigraph GT3X accelerometer (Actigraph, Pensacola, FL, USA) that children were instructed to wear for 24h during 7 conssecutive days. Finally, physical fitness (speed and agility, explosive power of legs, handgrip strength, flexibility and balance) were assessed through validated and standardized testing batteries.

Results

CEFIP was easily detected and measured in all saliva samples (mean concentration: 0.6 ± 0.2 pg/ml). Salivary CEFIP was positively associated with D/S BP ratio (r=0.305, p=0.010) and sedentary time (r=0.317, p=0.012), but negatively associated with PA in 7-9 years old school-children (r=-0.350, p=0.002). Furthermore, salivary CEFIP was related to lower level of balance i.e., higher center of pressure (CoP) displacement in these children (r=0.411, p<0.001). The associations of salivary CEFIP with D/S BP ratio (Beta=0.349, p=0.004), sedentary time (Beta=0.354, p=0.009) and CoP displacement (Beta=0.401, p=0.001), were maintained significant after adjustment for potential confounding variables such as age, gender and BMI in linear regression analyses.

Conclusion

CEFIP can be easily assessed in saliva as a promising biomarker associated with cardiovascular health in 7-9 years old school-children. Interestingly, higher salivary CEFIP concentration was related to higher D/S BP ratio, more sedentary time and higher CoP displacement i.e., lower level of balance in these children.
Biochar addition to compost heat recovery systems improves heat conversion yields
Authors: Pivato Alberto , Malesani Rachele , Bocchi Stefano , Rafieenia Razieh , Schievano Andrea
Published: January 2024

Introduction: Compost heat recovery systems (CHRS) represent an emerging technology to recover residual woody biomass from agroforestry and forestry activities and use the heat that is naturally produced during aerobic biodegradation (composting). However, a low oxygen concentration in the gas phase and self-drying and compaction of the compost body often limit efficient oxidation by microbial communities. Woodchip-derived biochar has often been proposed as a bulking agent and improver of water retention and of oxygen accessibility in the composting process, but the literature reporting its effects in the CHRS is scarce.

Methods: Here, biochar (average particle size of 10 mm) was added at 10% (on weight basis) to chipped pruning residues into two bench-scale-controlled reactors (0.2 m3), operated in parallel for 57 days.

Results and Discussion: The addition of 10% (w/w) biochar to the composting body increased biodegradation yields by approximately 50% and improved oxidation rates over readily biodegradable organic fractions (addition of cheese whey). Temperatures were on average 1.34°C higher, and heat extraction flux was also improved in the presence of biochar (0.3 kW/m3) versus in its absence (0.1 kW/m3). The organic matter mass balance resulted in approximately 50% higher biodegradation yield and improved oxidation rates over readily biodegradable organic fractions. Microbial analysis highlighted a higher concentration of thermophilic species and a lower concentration of well-known pathogenic and antibiotic-resistant genera in the presence of biochar.
Thermal gradient strategy to improve seeding for high rate zero excess lithium metal batteries
Authors: Raj Abhi , Atkinson Robert W. , Kingston Todd A. , Carter Rachel , Love Corey T.
Published: January 2024

Zero excess lithium metal batteries (LMBs) have traditionally suffered from short cycle life due to nonuniform processes that result in parasitic side reactions and a subsequent loss of lithium inventory and electrolyte. The experiments herein demonstrate that zero excess LMB cells cycled with a low thermal average and thermal gradient outperform cells cycled under isothermal conditions during early cycles. Specifically, a low thermal average of ∼6.4°C and thermal gradient of <1°C across the cell is shown to increase the overpotential for lithium deposition at the anode current collector, likely resulting in smaller and higher density nucleates, providing film like morphologies observed with microscopy. Improved performance from this approach is demonstrated at high cycling rates (>4C) and mismatched charge/discharge rates. Optimal cycling behavior was observed with 2C charging (30 min) and 3C discharging (20 min). These advantages were translated to the system relevant form factor-pouch cell (20X capacity). Based on the performance enhancement observed with extended application of a thermal gradient, we demonstrate the use of the environment as a formation strategy, to perpetuate improved plating in stripping over the cycle life of zero excess LMBs operating in ambient conditions.
Short-term wind power forecasting based on dual attention mechanism and gated recurrent unit neural network
Authors: Xu Wu , Liu Yang , Fan Xinhao , Shen Zhifang , Wu Qingchang
Published: January 2024

Accurate wind power forecasting is essential for both optimal grid scheduling and the massive absorption of wind power into the grid. However, the continuous changes in the contribution of various meteorological features to the forecasting of wind power output under different time or weather conditions, and the overlapping of wind power sequence cycles, make forecasting challenging. To address these problems, a short-term wind power forecasting model is established that integrates a gated recurrent unit (GRU) network with a dual attention mechanism (DAM). To compute the contributions of different features in real time, historical wind power data and meteorological information are first extracted using a feature attention mechanism (FAM). The feature sequences collected by the FAM are then used by the GRU network for preliminary forecasting. Subsequently, one-dimensional convolution employing several distinct convolution kernels is used to filter the GRU outputs. In addition, a multi-head time attention mechanism (MHTAM) is proposed and a Gaussian bias is introduced to assign different weights to different time steps of each modality. The final forecast results are produced by combining the outputs of the MHTAM. The results of the simulation experiment show that for 5-h, 10-h, and 20-h short-term wind power forecasting, the establishedÂ DAM-GRU model performs better than comparative models on the basis of Root Mean Square Error (RMSE), Mean Absolute Error (MAE), R-squared (R2), Square sum error (SSE), Mean absolute percentile error (MAPE), and Relative root mean square error (RRMSE) index.
Techno-economic evaluation of meshed distribution network planning with load growth and expansion with multiple assets across multiple planning horizons
Authors: Altamimi Abdullah
Published: January 2024

The smart grid paradigm has ushered in an era where modern distribution systems are expected to be both robust and interconnected in topology. This paper presents a techno-economic-based sustainable planning (TESP) strategy, which can be used as a planning framework for linked distribution systems, seeking to discover a realistic solution among competing criteria of diverse genres. In this comparative analysis-based study, three voltage stability assessment indices—VSA_A, VSA_B, and VSA_W—and a loss minimization condition (LMC)-based framework are used in the initial stage to achieve optimal distributed generation (DG)-based asset optimization for siting, followed by sizing. The respective techniques are evaluated across two variants of multiple load growth horizons spread across 10 years. The suggested TESP technique is tested on two variants of a mesh-configured microgrid (MCMG) with varied load growth scenarios. One variant considers a 65-bus MG with a fixed load growth of 2.7% across two load growth horizons. The other variant considers a 75-bus MG with varied load growth across four load growth horizons, encapsulating an expansion-based planning perspective. The numerical results of the suggested TESP approach in a comparative study demonstrate its effectiveness, and it can be used by researchers and planning engineers as a planning framework for interconnected distribution tools across multiple planning horizons. The proposed study would contribute to enhancing the robustness and interconnectivity of smart grid distribution systems. This dual focus could lead to more cost-effective and reliable power distribution systems.
The perturbation of PAHs on mosses in Aberdeen, Scotland, as a consequence of social restrictions imposed during the COVID-19 pandemic
Authors: Daniya Thomas S. , Bowden Stephen A.
Published: January 2024

Air quality and the concentration of polycyclic aromatic hydrocarbons (PAHs) in urban environments are increasingly becoming a concern. Measuring air quality and PAHs, and linking these to specific activities, requires deploying and recovering sampling devices to capture and measure any changes. From May 2020 to June 2021, during the “lockdown” period of the COVID-19 pandemic, acrocarpous and pleurocarpous mosses were collected from five types of site in Aberdeen, United Kingdom, and the concentration of PAHs measured to track changes in PAHs as travel-related activities changed. The predominant types of PAHs found on mosses were of the four- and five-ring varieties, with the four-ring variety of PAH (medium molecular weight) becoming more dominant as the lockdown was lifted. However, for most sites, the concentration of PAHs was evidently perturbed by the transient lifting, reimposing, and then lifting of lockdown conditions, which either directly limited travel or changed people’s motivations to travel by car. Molecular diagnostic ratios or molecular marker parameters used to infer the source of PAHs varied little and were nearly always consistent with PAHs deriving from combustion in vehicle engines. Thus, even when travel was limited, PAHs were still derived from vehicle usage, although the overall concentrations on mosses were much lower. On average, the lowest PAH concentrations were found on mosses collected from a recreational park located the furthest from traffic. The highest PAH concentrations were observed on mosses collected from residential car parks during periods when the lockdown had been lifted. However, mosses from the same residential car parks had very low PAH concentrations during periods of travel restrictions, strongly suggesting that, for mosses, local factors and patterns of vehicle usage strongly determine their exposure to PAHs. Therefore, mosses within urban environments can be used for monthly monitoring of PAHs as they are able to detect changes induced by human behaviour.
Complement activation and cellular inflammation in Fabry disease patients despite enzyme replacement therapy
Authors: Laffer Björn , Lenders Malte , Ehlers-Jeske Elvira , Heidenreich Karin , Brand Eva , Köhl Jörg
Published: January 2024

Defective α-galactosidase A (AGAL/GLA) due to missense or nonsense mutations in the GLA gene results in accumulation of the glycosphingolipids globotriaosylceramide (Gb3) and its deacylated derivate globotriaosylsphingosine (lyso-Gb3) in cells and body fluids. The aberrant glycosphingolipid metabolism leads to a progressive lysosomal storage disorder, i. e. Fabry disease (FD), characterized by chronic inflammation leading to multiorgan damage. Enzyme replacement therapy (ERT) with agalsidase-alfa or -beta is one of the main treatment options facilitating cellular Gb3 clearance. Proteome studies have shown changes in complement proteins during ERT. However, the direct activation of the complement system during FD has not been explored. Here, we demonstrate strong activation of the complement system in 17 classical male FD patients with either missense or nonsense mutations before and after ERT as evidenced by high C3a and C5a serum levels. In contrast to the strong reduction of lyso-Gb3 under ERT, C3a and C5a markedly increased in FD patients with nonsense mutations, most of whom developed anti-drug antibodies (ADA), whereas FD patients with missense mutations, which were ADA-negative, showed heterogenous C3a and C5a serum levels under treatment. In addition to the complement activation, we found increased IL-6, IL-10 and TGF-ß1 serum levels in FD patients. This increase was most prominent in patients with missense mutations under ERT, most of whom developed mild nephropathy with decreased estimated glomerular filtration rate. Together, our findings demonstrate strong complement activation in FD independent of ERT therapy, especially in males with nonsense mutations and the development of ADAs. In addition, our data suggest kidney cell-associated production of cytokines, which have a strong potential to drive renal damage. Thus, chronic inflammation as a driver of organ damage in FD seems to proceed despite ERT and may prove useful as a target to cope with progressive organ damage.
Three-dimensional numerical study of the deep western boundary current in the South China Sea
Authors: Zhou Muping , Ling Zheng , Chen Changlin
Published: January 2024

This study utilized a three-dimensional ocean general circulation model to investigate the intensity, thickness, and width of the three-dimensional deep western boundary current (DWBC) in the South China Sea (SCS). The numerical results show that the DWBC begins near the inlet of the Luzon overflow, flows westward along the northern boundary, proceeds southward along the western boundary, and ultimately terminates at the southern boundary. The mean DWBC’s velocity, thickness, and width is 4.78 cm/s, 1645 m, and 140 km, respectively. Combined with the dynamic results, it is evident that the three-dimensional structure of the DWBC appears to have been visibly weakened after the closure of the deep Luzon overflow. Strong deep mixing has a significantly stronger, thicker, and wider effect on the intensity, thickness, and width of the DWBC. Both the bottom and lateral friction coefficients negatively impact the DWBC in the SCS.
Multiple cystic echinococcosis in abdominal and pelvic cavity treated by surgery with a 4-year follow-up: a case report
Authors: Yan Lerong , Chu Zhiqiang , Yang Jian , Zhang Yongguo , Liu Guisheng , Lei Zhen , Chen Qian , Li Jiang , Yang Jing , Zhao Meifeng , Zhang Shijie , Wu Xiangwei , Peng Xinyu , Zhang Hongwei
Published: January 2024

We report a case of a male patient who presented with multiple abdominal and pelvic echinococcosis. The patient had been diagnosed with hepatic echinococcosis for 7 years and developed intermittent distension and discomfort in the upper abdomen after an accidental fall. In recent years, the patient’s abdominal distention increased gradually. Computed tomography revealed multiple hydatid cysts in the liver, spleen, abdominal cavity, and pelvic cavity. Abdominal organs were severely compressed, such that he could not eat normally except for a liquid diet. The patient underwent radical surgical resection based on the multi-disciplinary treatment (MDT) and the operation lasted 10 h, nearly 100 hydatid cysts were excised, about 18 liters of cyst fluid and cyst contents were removed, and the patient lost 20 kg of weight after surgery. The operation was successful, but there were still some postoperative complications such as hypovolemic shock, postoperative ascites, postoperative bile leakage. Treatment measures for the patient were anti-infection, antishock, clamping the abdominal drainage tube, and negative pressure abdominal puncture drainage. At follow up the patient’s quality of life had been significantly improved with 15 kg weight gain compared to before.
Characterization of MdpS: an in-depth analysis of a MUC5B-degrading protease from Streptococcus oralis
Authors: Leo Fredrik , Lood Rolf , Thomsson Kristina A. , Nilsson Jonas , Svensäter Gunnel , Wickström Claes
Published: January 2024

Oral biofilms, comprising hundreds of bacteria and other microorganisms on oral mucosal and dental surfaces, play a central role in oral health and disease dynamics. Streptococcus oralis, a key constituent of these biofilms, contributes significantly to the formation of which, serving as an early colonizer and microcolony scaffold. The interaction between S. oralis and the orally predominant mucin, MUC5B, is pivotal in biofilm development, yet the mechanism underlying MUC5B degradation remains poorly understood. This study introduces MdpS (Mucin Degrading Protease from Streptococcus oralis), a protease that extensively hydrolyses MUC5B and offers an insight into its evolutionary conservation, physicochemical properties, and substrate- and amino acid specificity. MdpS exhibits high sequence conservation within the species and also explicitly among early biofilm colonizing streptococci. It is a calcium or magnesium dependent serine protease with strict physicochemical preferences, including narrow pH and temperature tolerance, and high sensitivity to increasing concentrations of sodium chloride and reducing agents. Furthermore, MdpS primarily hydrolyzes proteins with O-glycans, but also shows activity toward immunoglobulins IgA1/2 and IgM, suggesting potential immunomodulatory effects. Significantly, MdpS extensively degrades MUC5B in the N- and C-terminal domains, emphasizing its role in mucin degradation, with implications for carbon and nitrogen sequestration for S. oralis or oral biofilm cross-feeding. Moreover, depending on substrate glycosylation, the amino acids serine, threonine or cysteine triggers the enzymatic action. Understanding the interplay between S. oralis and MUC5B, facilitated by MdpS, has significant implications for the management of a healthy eubiotic oral microenvironment, offering potential targets for interventions aimed at modulating oral biofilm composition and succession. Additionally, since MdpS does not rely on O-glycan removal prior to extensive peptide backbone hydrolysis, the MdpS data challenges the current model of MUC5B degradation. These findings emphasize the necessity for further research in this field.
Standardizing default electronic health record tools to improve safety for hospitalized patients with Parkinson’s disease
Authors: Wu Allan D. , Walter Benjamin L. , Brooks Anne , Buetow Emily , Amodeo Katherine , Richard Irene , Mundth Kelly , Azmi Hooman
Published: January 2024

Electronic Health Record (EHR) systems are often configured to address challenges and improve patient safety for persons with Parkinson’s disease (PWP). For example, EHR systems can help identify Parkinson’s disease (PD) patients across the hospital by flagging a patient’s diagnosis in their chart, preventing errors in medication and dosing through the use of clinical decision support, and supplementing staff education through care plans that provide step-by-step road maps for disease-based care of a specific patient population. However, most EHR-based solutions are locally developed and, thus, difficult to scale widely or apply uniformly across hospital systems. In 2020, the Parkinson’s Foundation, a national and international leader in PD research, education, and advocacy, and Epic, a leading EHR vendor with more than 35% market share in the United States, launched a partnership to reduce risks to hospitalized PWP using standardized EHR-based solutions. This article discusses that project which included leadership from physician informaticists, movement disorders specialists, hospital quality officers, the Parkinson’s Foundation and members of the Parkinson’s community. We describe the best practice solutions developed through this project. We highlight those that are currently available as standard defaults or options within the Epic EHR, discuss the successes and limitations of these solutions, and consider opportunities for scalability in environments beyond a single EHR vendor. The Parkinson’s Foundation and Epic launched a partnership to develop best practice solutions in the Epic EHR system to improve safety for PWP in the hospital. The goal of the partnership was to create the EHR tools that will have the greatest impact on outcomes for hospitalized PWP.
Multiple sclerosis clinical forms classification with graph convolutional networks based on brain morphological connectivity
Authors: Chen Enyi , Barile Berardino , Durand-Dubief Françoise , Grenier Thomas , Sappey-Marinier Dominique
Published: January 2024

Multiple Sclerosis (MS) is an autoimmune disease that combines chronic inflammatory and neurodegenerative processes underlying different clinical forms of evolution, such as relapsing-remitting, secondary progressive, or primary progressive MS. This identification is usually performed by clinical evaluation at the diagnosis or during the course of the disease for the secondary progressive phase. In parallel, magnetic resonance imaging (MRI) analysis is a mandatory diagnostic complement. Identifying the clinical form from MR images is therefore a helpful and challenging task. Here, we propose a new approach for the automatic classification of MS forms based on conventional MRI (i.e., T1-weighted images) that are commonly used in clinical context. For this purpose, we investigated the morphological connectome features using graph based convolutional neural network. Our results obtained from the longitudinal study of 91 MS patients highlight the performance (F1-score) of this approach that is better than state-of-the-art as 3D convolutional neural networks. These results open the way for clinical applications such as disability correlation only using T1-weighted images.
Athletes’ nutritional demands: a narrative review of nutritional requirements
Authors: Amawi Adam , AlKasasbeh Walaa , Jaradat Manar , Almasri Amani , Alobaidi Sondos , Hammad Aya Abu , Bishtawi Taqwa , Fataftah Batoul , Turk Nataly , Saoud Hassan Al , Jarrar Amjad , Ghazzawi Hadeel
Published: January 2024

Nutrition serves as the cornerstone of an athlete’s life, exerting a profound impact on their performance and overall well-being. To unlock their full potential, athletes must adhere to a well-balanced diet tailored to their specific nutritional needs. This approach not only enables them to achieve optimal performance levels but also facilitates efficient recovery and reduces the risk of injuries. In addition to maintaining a balanced diet, many athletes also embrace the use of nutritional supplements to complement their dietary intake and support their training goals. These supplements cover a wide range of options, addressing nutrient deficiencies, enhancing recovery, promoting muscle synthesis, boosting energy levels, and optimizing performance in their respective sports or activities. The primary objective of this narrative review is to comprehensively explore the diverse nutritional requirements that athletes face to optimize their performance, recovery, and overall well-being. Through a thorough literature search across databases such as PubMed, Google Scholar, and Scopus, we aim to provide evidence-based recommendations and shed light on the optimal daily intakes of carbohydrates, protein, fats, micronutrients, hydration strategies, ergogenic aids, nutritional supplements, and nutrient timing. Furthermore, our aim is to dispel common misconceptions regarding sports nutrition, providing athletes with accurate information and empowering them in their nutritional choices.
Assessment of compression forces in a digitally modified short leg cast for pressure injury risk monitoring in healthy children
Authors: Vrankovic Matia Iva , Annaheim Simon , Willibald Jana , Lieber Jan , van Hedel Hubertus J. A. , Schlüer Anna-Barbara , Rossi René M. , Meyer-Heim Andreas
Published: January 2024

Introduction

Casting is an essential treatment for neuro-orthopedic conditions in children with cognitive, sensory, and communicational disabilities. However, a main side-effect is the development of pressure injuries resulting in additional (wound) therapies and prolongation of the hospital stay. The primary aim of our study was to investigate the potential of objective pressure measurements in casts to assess the risk for pressure injury development.

Methods

Five pediatric healthy participants were included in this study. We measured the global and the local compression force at body sites prone to pressure injury development for different body positions and the transfer in-between in a cast equipped with pressure sensors. These conditions resulted in partial or full body weight loading.

Results and discussion

The global maximum compression force was affected significantly by body postures with partial and full loading of the cast and during transfer. The local compression force significantly correlated with the global compression force at the heel and instep area. In conclusion, the integration of sensing technologies into casts bears a high potential for early recognition of critical conditions inside the cast and inducing preventive measures in the at-risk population.
Case Report: Successful avoidance of etoposide for primary hemophagocytic lymphohistiocytosis-induced multiple organ dysfunction syndrome using emapalumab
Authors: Hahn Timothy J. , McKeone Daniel J. , Beal James W. , Ericson Jessica E. , Halstead E. Scott
Published: January 2024

We describe the case of an infant who presented with simple rhinovirus/enterovirus bronchiolitis whose condition worsened with rapid progression to multiple organ dysfunction syndrome (MODS). The patient was presumed to have either primary or secondary hemophagocytic lymphohistiocytosis (HLH), and treatment was initiated using dexamethasone, anakinra, and intravenous immunoglobulin to modulate the immune system. Due to the organ dysfunction, the use of etoposide was avoided and instead, emapalumab, an interferon gamma antagonist, was administered at a dose of 6 mg/kg. The patient's organ failure improved, and the levels of inflammatory markers decreased. The flow cytometry analysis revealed that cytotoxic cells lacked perforin expression, and subsequent genetic analysis confirmed homozygous pathogenic mutations in the perforin gene. This case highlights the potential avoidance of etoposide in cases of primary HLH, the possible benefit of an elevated initial dose of emapalumab, and the contribution offered by a multi-specialty team approach to complex diagnosis.
Unidirectional barbed sutures vs. interrupted intracorporeal knots in thoracoscopic repair of congenital diaphragmatic hernia in pediatrics
Authors: Shehata Mohamed Ali , Negm Mohamed Ahmed , Shalaby Mohamed Mahmoud , Mansour Mohamed Awad , Elhaddad Ahmed Abdelmhaimen
Published: January 2024

Background

Intracorporeal suturing knots continue to be one of the most challenging and time-consuming steps in the thoracoscopic repair of congenital diaphragmatic hernia (CDH). Barbed unidirectional knotless sutures are designed to shorten surgical procedures by eliminating the need to tie knots. This work aimed to compare unidirectional barbed sutures and interrupted intracorporeal knots in the thoracoscopic repair of CDH in pediatrics regarding the time required to suture, operative time and complications.

Methods

This retrospective study included 139 patients presented with Bochdalek CDH. Patients were classified into early (neonatal) and late presentations. The hernia defect was repaired by unidirectional Barbed sutures (V-LocTM and StratafixTM sutures) in group B or by Conventional interrupted intracorporeal knots in group C.

Results

In both early and delayed presentations, the time required to suture (15 and 13 min in group B, 33 and 28 min in group C for neonatal and delayed presentation respectively) was significantly shorter in group B. Complications (visceral perforation, wound infection, and recurrence) insignificantly differed between group B and group C of early presentation. No patients suffered from major complications in both groups.

Conclusions

Both unidirectional barbed sutures and intracorporeal knots were safe and effective. However, unidirectional barbed sutures are a time-saving choices for CDH thoracoscopic repair in early and late presentations.
Molecular and biochemical responses of sesame (Sesame indicum L.) to rhizobacteria inoculation under water deficit
Authors: dos Santos Anderson Reges , da Rocha Geisenilma Maria Gonçalves , Machado Alexandre Paulo , Fernandes-Junior Paulo Ivan , Arriel Nair Helena Castro , Gondim Tarcisio Marcos de Souza , de Lima Liziane Maria
Published: January 2024

Introduction

Water scarcity is a challenge for sesame cultivation under rainfed conditions. In this scenario, a potential strategy to alleviate the water deficit is the application of plant growth-promoting bacteria. The objective of this study was to analyze the interaction of rhizobacteria with sesame cultivation under water deficit conditions.

Methods

An experiment was conducted in pots in a greenhouse using the BRS Morena sesame cultivar. The experimental design was completely randomized in a factorial scheme: 2 (irrigation regimes - daily irrigation and water deficit by suspending irrigation until 90% stomatal closure) x 6 (treatments with nitrogen or inoculants), with 5 replications. The types of fertilization were characterized by the addition of nitrogen (ammonium sulfate; 21% N), inoculants based on Bacillus spp. (pant001, ESA 13, and ESA 402), Agrobacterium sp. (ESA 441), and without nitrogen (control). On the fifth day after the suspension of irrigation, plant material was collected for gene expression analysis (DREB1 and HDZ7), activities of antioxidant enzymes (superoxide dismutase and catalase), relative proline content, and photosynthetic pigments. At the end of the crop cycle (about 85 days), production characteristics (root dry matter, aboveground dry matter, number of capsules, and thousand seed weight), as well as leaf nitrogen (N) and phosphorus (P) content, were evaluated.

Results and Discussion

There was a positive effect on both production and biochemical characteristics (proline, superoxide dismutase, catalase, and photosynthetic pigments). Regarding gene expression, most of the inoculated treatments exhibited increased expression of the DREB1 and HDZ7 genes. These biological indicators demonstrate the potential of rhizobacteria for application in sesame cultivation, providing nutritional supply and reducing the effects of water deficit.
Identifying genetically redundant accessions in the world’s largest cassava collection
Authors: Carvajal-Yepes Monica , Ospina Jessica A. , Aranzales Ericson , Velez-Tobon Monica , Correa Abondano Miguel , Manrique-Carpintero Norma Constanza , Wenzl Peter
Published: January 2024

Crop diversity conserved in genebanks facilitates the development of superior varieties, improving yields, nutrition, adaptation to climate change and resilience against pests and diseases. Cassava (Manihot esculenta) plays a vital role in providing carbohydrates to approximately 500 million people in Africa and other continents. The International Center for Tropical Agriculture (CIAT) conserves the largest global cassava collection, housing 5,963 accessions of cultivated cassava and wild relatives within its genebank. Efficient genebank management requires identifying and eliminating genetic redundancy within collections. In this study, we optimized the identification of genetic redundancy in CIAT’s cassava genebank, applying empirical distance thresholds, and using two types of molecular markers (single-nucleotide polymorphism (SNP) and SilicoDArT) on 5,302 Manihot esculenta accessions. A series of quality filters were applied to select the most informative and high-quality markers and to exclude low-quality DNA samples. The analysis identified a total of 2,518 and 2,526 (47 percent) distinct genotypes represented by 1 to 87 accessions each, using SNP or SilicoDArT markers, respectively. A total of 2,776 (SNP) and 2,785 (SilicoDArT) accessions were part of accession clusters with up to 87 accessions. Comparing passport and historical characterization data, such as pulp color and leaf characteristic, we reviewed clusters of genetically redundant accessions. This study provides valuable guidance to genebank curators in defining minimum genetic-distance thresholds to assess redundancy within collections. It aids in identifying a subset of genetically distinct accessions, prioritizing collection management activities such as cryopreservation and provides insights for follow-up studies in the field, potentially leading to removal of duplicate accessions.
Establishment of an experimental model of ovalbumin-induced atopic dermatitis in canines
Authors: Shin Ha-Young , Jin Hyung Jun , Tae Hyun-Jin , Oh Hong-Geun , Hwang Jeong Ho
Published: January 2024

Introduction

A reliable standard model is required to evaluate the efficacy of new drugs for companion animals, especially dogs. Canine atopic dermatitis (cAD), also known as allergic inflammatory skin disease, is a common condition. Currently, the house dust mite animal model is used in the research of cAD; however, this model exhibits significant individual variation and is difficult to standardize. In this study, we used ovalbumin as an antigen to sensitize and stimulate dogs, thereby establishing a stable model mimicking the T-helper 2 (Th2) response seen in cAD. Our objective was to create a cAD model that could be employed to evaluate the efficacy of novel drugs and mimic the Th2 dominant allergic response observed in the pathogenesis of atopic dermatitis of dogs.

Methods

In this study, six beagles were used. Normal saline was applied to two animals, and ovalbumin to four, on their dorsal skin.

Results

The ovalbumin-treated groups exhibited clinical cAD symptoms, such as pruritus and erythema. Moreover, plasma levels of the cAD markers immunoglobulin E and CCL17 chemokine were higher in the ovalbumin-treated group than in the vehicle control group. The skin thickness of the epidermis was significantly increased in the ovalbumin-treated group, with infiltration of inflammatory cells observed in the thickened dermis region. In conclusion, treatment of canine skin with an optimal concentration of ovalbumin induced typical cAD-like symptoms, and histological and molecular analyses confirmed an enhanced Th2-related immune response.

Conclusion

Therefore, we successfully established a suitable Th2-dominant response mimicking cAD, which will facilitate targeted research of atopic dermatitis in dogs.
Numerical Study on the Corner Separation Control for a Compressor Cascade via Bionic Herringbone Riblets
Authors: Zhang Peng , Cheng Rixin , Li Yonghong
Published: January 2024

Bionic herringbone riblets are applied to relieve the flow near the blade endwall in a linear compressor cascade under the incidence angle of −4° to 6° at a Reynolds number of 382,000. The herringbone riblets are placed at the endwall upstream of the blade, and the Reynolds-averaged Navier–Stokes simulations are performed to explore their effects on corner separation and the control mechanism. The results show that the herringbone riblets can effectively improve the corner separation over the stable operating range, and the control effect is affected by the riblet height and the yaw angle. The implementation of herringbone riblets with a height of only 0.08 boundary layer thickness and a yaw angle of 30 degrees can reduce the total pressure loss by up to 9.89% and increase the static pressure coefficient by 12.27%. Flow details indicate that small-scale vortices in the riblet channels can accumulate and form a high-intensity large-scale vortex close to the bottom of the boundary layer downstream. Compared with traditional vortex generators, the herringbone riblets induce a vortex closer to the wall due to their smaller size, which can reduce the damage of an induced vortex to the mainstream and enhance its control over the bottom of the boundary layer, thereby effectively reducing additional losses. The induced vortex enhances mixing and injects kinetic energy into the low-energy fluid, thus inhibiting the transverse migration of low-energy fluid in the endwall boundary layer, delaying the formation of the separating vortex, further suppressing the development of corner separation and improving the aerodynamic performance of the cascade.
Evaluation of the Multiaxial Fatigue Life of Electro-Mechanical Actuator for Aircraft Blade Pitch Control Based on Certification Standards
Authors: Kim Young-Cheol , Kim Dong-Hyeop , Kim Sang-Woo
Published: January 2024

To achieve the commercialization of electric vertical takeoff and landing (eVTOL) aircrafts, which have recently garnered attention as the next-generation means of transportation, objective certification based on rigorous procedures is essential. With the advancement of structural analysis technology, aircraft airworthiness standards recommend a combination of testing and analytical methods to demonstrate structural integrity. In this study, we propose analytical techniques for demonstrating the structural integrity of components for eVTOL aircrafts in accordance with airworthiness standards. We evaluated the static structural integrity and fatigue safety of an electro-mechanical actuator. Multibody dynamics analysis was performed to calculate the loads for application in finite element analysis. Subsequently, static analysis and fatigue analysis based on finite element analysis were conducted to calculate the safety margin and fatigue life of all key components. Therefore, we have confirmed the feasibility of utilizing analytical methods for the structural integrity assessment of aircraft components. We propose the utilization of the technique introduced in this study as one of the approaches for demonstrating compliance with airworthiness standards for eVTOL aircrafts through the application of analytical methods.
Genomic Insights into Seed Germination Differences in Buffalobur (Solanum rostratum Dunal) under Contrasting GA and ABA Availability
Authors: Chen Zhaoxia , Li Longlong , Wu Kaidie , Zhao Dandan , Yang Long , Huang Hongjuan , Huang Zhaofeng , Wei Shouhui
Published: January 2024

Buffalobur (Solanum rostratum Dunal) is an invasive species that seriously endangers crop production and the ecological environment. Seeds are the primary source of infestation; therefore, understanding the molecular basis of buffalobur seed dormancy, and germination is crucial for precision weed management. In this study, high-throughput RNA-Seq was performed on buffalobur seeds, which imbibed under 0.35 mmol/L giberellic acid (GA) and 0.35 mmol/L abscisic acid (ABA). In total, 3658 differentially expressed genes (DEGs) were identified during seed germination. Gene annotation revealed that the DEGs were significantly enriched during the protein metabolic process, as well as the macromolecular complex and cytoplasmic part for ABA versus GA. Pathway analysis predicted that the DEGs were associated with metabolic pathways, the biosynthesis of secondary metabolites and ribosome. Nine germination-related genes involved in the biosynthesis and metabolism of the phytohormones and encoding of the endo-β-mannanase (EBM) were identified. Gene expression indicated that GA upregulated GA3OX1 and MAN2 expression to increase the EBM activity, which caused the endosperm cap to weaken and lowered the puncture force to trigger the germination of buffalobur. The obtained results would be helpful to clarify the regulation of seed dormancy and the germination of buffalobur, and could serve as a valuable resource when unravelling the genetic basis of seed biology of this weed species.
Effects of Different Irrigation Management and Nitrogen Rate on Sorghum (Sorghum bicolor L.) Growth, Yield and Soil Nitrogen Accumulation with Drip Irrigation
Authors: Wang Zelin , Nie Tangzhe , Lu Dehao , Zhang Peng , Li Jianfeng , Li Fanghao , Zhang Zhongxue , Chen Peng , Jiang Lili , Dai Changlei , Waller Peter M.
Published: January 2024

Sorghum (Sorghum bicolor L.) has emerged as a pivotal global food crop. Consequently, it is imperative to explore sustainable and eco-friendly strategies to achieve sustainable sorghum production with a high yield. This study aimed to reveal the effects of irrigation management and nitrogen rates and their interactions on sorghum growth traits, yield and soil nitrate-N and ammonium-N accumulation to improve irrigation and nitrogen practices under drip irrigation. A 2-year (2021 and 2022) field experiment was conducted on drip-irrigated fertilized sorghum in Heilongjiang Province to investigate the effects of three lower levels of soil moisture (80% (HI), 70% (NI), and 60% (LI) of field capacity) with four nitrogen rates at 225, 150, 75 and 0 kg/ha (designated as HN, NN, LN and WN, respectively) on sorghum growth, yield and soil nitrogen accumulation. The results indicated that irrigation management and nitrogen rate interaction had a significant effect on sorghum growth (plant height, stem diameter, leaf area index (LAI), and SPAD value), yield, aboveground biomass and 0~60 cm soil nitrogen accumulation (p < 0.05). The NNHI treatment demonstrated the highest plant height (120.9 and 121.8 cm) and LAI (2.738 and 2.645) in 2021 and 2022, and there was a significant positive correlation between plant height, LAI, and yield (p < 0.01). However, the NNNI treatment exhibited the highest yield (7477.41 and 7362.27 kg/ha) in 2021 and 2022, sorghum yield increased and then decreased with an increase in irrigation management and nitrogen rate. In addition, soil nitrate-N and ammonium-N accumulation were significantly affected by the interaction of irrigation management and nitrogen rate (p < 0.05) while irrigation management had no significant effect on the accumulation of nitrate-N and ammonium-N. Soil nitrate-N and ammonium-N accumulation increased with the increasing nitrogen rate. Although yield differences between the NNNI and HNNI treatments were not significant, the NNNI treatment with a lower soil moisture limit of 70% field capacity and a nitrogen rate of 150 kg/ha accumulated 10.4% less nitrate-N in soil than the HNNI treatment, reduced risk of nitrate nitrogen leaching. The regression analysis indicated that the optimal irrigation management and nitrogen rate management practices of 71.93% of the soil moisture lower limit and 144.58 kg/ha of nitrogen rate was an optimal strategy for favorable sorghum growth, high-yielding and low soil nitrate-N accumulation of sorghum. This study provides a scientific reference for precise water and fertilizer management in sorghum.
Opposite Responses of Native and Nonnative Birds to Socioeconomics in a Latin American City
Authors: Villaseñor Nélida R. , Muñoz-Pacheco Catalina B. , Escobar Martín A. H.
Published: January 2024

Due to the massive increase of the urban population, a global target is to achieve sustainable cities that are sensitive to nature and environmentally just for urban dwellers. To accomplish this, it is important to understand the responses of native and nonnative birds, identify the environmental variables that promote native species and limit nonnative species, and understand how they vary among socioeconomic groups. Although many cities in the Global South exhibit strong social and environmental segregation, few studies have investigated the relationship between socioeconomics and biodiversity. Therefore, to help promote ecological justice and biodiversity conservation in the developing world, we investigated the influence of socioeconomic level and woody cover on bird species richness and abundance in the city of Santiago de Chile. We also investigated whether bird response changes with species provenance—it is important to understand the response of native birds separately from nonnative birds because they imply opposite management strategies (e.g., conservation vs. species control/eradication). Thus, we surveyed 120 sites located in residential areas of high, medium, and low socioeconomic levels across the city and fit generalized linear (mixed) models that described bird species richness and abundance for total, native, and nonnative birds according to socioeconomic level and woody vegetation cover. We found that both socioeconomic level and woody vegetation cover influenced the bird community, but their effects changed with bird species origin, having opposite effects on native and nonnative birds. Residential areas where wealthier people live supported greater species richness and abundance of native birds than residential areas where people of lower socioeconomic status live. In contrast, residential areas where vulnerable people live had greater bird abundance that was mainly composed of nonnative birds. Therefore, affluent neighborhoods provide more opportunities to encounter native birds and experience nature close to home than poorer neighborhoods. Due to woody cover having positive effects on native birds and a negative influence on nonnative birds, increasing tree and shrub cover will contribute to supporting more native birds in residential areas deprived of woody vegetation, which are commonly low socioeconomic areas. Additional variables that can explain bird response among residential areas of different socioeconomic levels need to be investigated to better understand the factors influencing the distribution of birds in cities and promote a more biodiverse and environmentally just city.
Assessing Different Chronic Wasting Disease Training Aids for Use with Detection Dogs
Authors: Mallikarjun Amritha , Charendoff Ila , Moore Madison B. , Wilson Clara , Nguyen Elizabeth , Hendrzak Abigail J. , Poulson Jean , Gibison Michelle , Otto Cynthia M.
Published: January 2024

Chronic wasting disease (CWD) is a highly infectious, fatal prion disease that affects cervid species. One promising method for CWD surveillance is the use of detection dog–handler teams wherein dogs are trained on the volatile organic compound signature of CWD fecal matter. However, using fecal matter from CWD-positive deer poses a biohazard risk; CWD prions can bind to soil particles and remain infectious in contaminated areas for extended periods of time, and it is very difficult to decontaminate the affected areas. One solution is to use noninfectious training aids that can replicate the odor of fecal matter from CWD-positive and CWD-negative deer and are safe to use in the environment. Trained CWD detection dogs’ sensitivity and specificity for different training aid materials (cotton, GetXent tubes, and polydimethylsiloxane, or PDMS) incubated with fecal matter from CWD-positive and CWD-negative deer at two different temperatures (21 °C and 37 °C) for three different lengths of time (6 h, 24 h, and 48 h) were evaluated. Cotton incubated at 21 °C for 24 h was identified as the best aid for CWD based on the dogs’ performance and practical needs for training aid creation. Implications for CWD detection training and for training aid selection in general are discussed.