bluuebunny/crossref_metadata_2025 huggingface dataset:
Salinity-Conveyed Thermotolerance in the Coral Model Aiptasia Is Accompanied by Distinct Changes of the Bacterial Microbiome
Authors: Randle Janna L. , Cárdenas Anny , Gegner Hagen M. , Ziegler Maren , Voolstra Christian R.
Published: November 2020

Coral bleaching, i.e., the loss of photosynthetic algal endosymbionts, caused by ocean warming is now among the main factors driving global reef decline, making the elucidation of factors that contribute to thermotolerance important. Recent studies implicate high salinity as a contributing factor in cnidarians, potentially explaining the high thermotolerance of corals from the Arabian Seas. Here we characterized bacterial community composition under heat stress at different salinities using the coral model Aiptasia. Exposure of two Aiptasia host-algal symbiont pairings (H2-SSB01 and CC7-SSA01) to ambient (25°C) and heat stress (34°C) temperatures at low (36 PSU), intermediate (39 PSU), and high (42 PSU) salinities showed that bacterial community composition at high salinity was significantly different, concomitant with reduced bleaching susceptibility in H2-SSB01, not observed in CC7-SSA01. Elucidation of bacteria that showed increased relative abundance at high salinity, irrespective of heat stress, revealed candidate taxa that could potentially contribute to the observed increased thermotolerance. We identified 4 (H2-SSB01) and 3 (CC7-SSA01) bacterial taxa belonging to the orders Alteromonadales (1 OTU), Oligoflexales (1 OTU), Rhizobiales (2 OTUs), and Rhodobacterales (2 OTUs), suggesting that only few bacterial taxa are potential contributors to an increase in thermal tolerance at high salinities. These taxa have previously been implicated in nitrogen and DMSP cycling, processes that are considered to affect thermotolerance. Our study demonstrates microbiome restructuring in symbiotic cnidarians under heat stress at different salinities. As such, it underlines how host-associated bacterial communities adapt to prevailing environmental conditions with putative consequences for the environmental stress tolerance of the emergent metaorganism.
Resource Partitioning Between Phytoplankton and Bacteria in the Coastal Baltic Sea
Authors: Sörenson Eva , Farnelid Hanna , Lindehoff Elin , Legrand Catherine
Published: November 2020

Eutrophication coupled to climate change disturbs the balance between competition and coexistence in microbial communities including the partitioning of organic and inorganic nutrients between phytoplankton and bacteria. Competition for inorganic nutrients has been regarded as one of the drivers affecting the productivity of the eutrophied coastal Baltic Sea. Yet, it is unknown at the molecular expression level how resources are competed for, by phytoplankton and bacteria, and what impact this competition has on the community composition. Here we use metatranscriptomics and amplicon sequencing and compare known metabolic pathways of both phytoplankton and bacteria co-occurring during a summer bloom in the archipelago of Åland in the Baltic Sea to examine phytoplankton bacteria resource partitioning. The expression of selected pathways of carbon (C), nitrogen (N), and phosphorus (P) metabolism varied over time, independently, for both phytoplankton and bacteria, indicating partitioning of the available organic and inorganic resources. This occurs regardless of eukaryotic plankton growth phase (exponential or stationary), based on expression data, and microbial community composition. Further, the availability of different nutrient resources affected the functional response by the bacteria, observed as minor compositional changes, at class level, in an otherwise taxonomically stable bacterial community. Resource partitioning and functional flexibility seem necessary in order to maintain phytoplankton-bacteria interactions at stable environmental conditions. More detailed knowledge of which organisms utilize certain nutrient species are important for more accurate projections of the fate of coastal waters.
Choosing a Surgical Access Point for Hysterectomy: A Paradigm Shift Over a 10-Year Span
Authors: Ebner Florian , de Gregorio Niko , Lato Christiane , Ohly Valerie , Janni Wolfgang , Spohrs Jennifer , Jerg-Bretzke Lucia , Walter Steffen
Published: November 2020

Background: When choosing a surgical procedure for a hysterectomy, doctors and patients have various options in terms of the multiple surgical access points available. The aim of this study was to descriptively analyze developments concerning the surgical access point selected over the past 10 years at Ulm University Hospital, (south) Germany, assess the variables associated with the surgical method and explore any potential significant correlations that influence these surgical access routes. Explicitly, we wished to investigate whether the approval of ulipristal acetate and the warning issued by the Food and Drug Administration (FDA) in connection with its use changed existing trends.

Material and Methods: This monocentric study retrospectively assessed data from all patients who underwent a hysterectomy due to a benign disease or endometrial cancer from January 2007 until December 2016.

Results: Of the benign indications considered, myomas and descensus genitalis occurred most frequently (49.5 and 30.6%, respectively). The percentage of abdominal procedures declined from 61.4 to 13.4% between 2007 and 2016 for all hysterectomies, whilst it increased from 4.1 to 69.7% for laparoscopic hysterectomies. The rate of vaginal hysterectomies increased to 45.5% until 2013 and declined in the years afterwards. Laparoscopic assisted vaginal hysterectomies were comparatively rare.

The trends in terms of surgical routes were similar for endometrial cancer. During the observation period, the share of abdominal hysterectomies fell from 100 to 11.3%, whilst the share of laparoscopic hysterectomies increased from 0 to 86.6%. The other two procedures were less frequently used.

Use of the laparoscopic hysterectomy procedure also increased significantly after the FDA's 2014 warning. Ulipristal acetate may have tended to influence the process.

Conclusion: Contrary to the national decrease in hysterectomy numbers, the annual number of hysterectomies at Ulm University Hospital remained stable during the observation period. Nevertheless, there was a clear shift in the preferred surgical routes for hysterectomy.
Dietary Montmorillonite Improves the Intestinal Mucosal Barrier and Optimizes the Intestinal Microbial Community of Weaned Piglets
Authors: Liu Han , Wang Congmin , Gu Xueling , Zhao Jing , Nie Cunxi , Zhang Wenju , Ma Xi
Published: November 2020

The study investigated the impact of dietary montmorillonite on the growth performance, intestinal mucosal barrier, and microbial community in weaned piglets with control group (CON) and dietary supplementation of 0.2% montmorillonite (0.2% M). Compared with the CON group, 0.2% M feed in the diet increased the average daily gain (ADG) on days 15–35 and day 1–35 and the average daily feed intake on days 1–35 (ADFI) (0.05 < P < 0.1). Besides, higher villus height of the duodenum and jejunum and lower crypt depth of duodenum and colon were revealed in the 0.2% M group than in the CON group (P < 0.05). Moreover, the V/C (ratio of the villus height and crypt depth) in the 0.2% M group was increased compared to that in the CON group both from the duodenum and ileum (P < 0.05). The relative mRNA expression of mucin-1, ITGB1 (β1-integrins), and PKC (protein kinase C) of ileum in the 0.2% M group were upregulated (P < 0.05) compared to that in the CON group. The digesta sample of ileum from piglets in the 0.2% M group contained greater (P < 0.05) intestinal bacterial diversity and abundances of probiotics, such as Streptococcus, Eubacterium_rectale_group, and Lactobacillus, which could promote the synthesis of carbon-containing biomolecules. Overall, dietary supplementation of 0.2% M was shown to have a tendency to improve the growth performance of weaned piglets and may enhance their intestinal mucosal barrier function via altering the gut microbiota.
The Multifaceted Nature of Streptococcal Antigen I/II Proteins in Colonization and Disease Pathogenesis
Authors: Manzer Haider S. , Nobbs Angela H. , Doran Kelly S.
Published: November 2020

Streptococci are Gram-positive bacteria that belong to the natural microbiota of humans and animals. Certain streptococcal species are known as opportunistic pathogens with the potential to cause severe invasive disease. Antigen I/II (AgI/II) family proteins are sortase anchored cell surface adhesins that are nearly ubiquitous across streptococci and contribute to many streptococcal diseases, including dental caries, respiratory tract infections, and meningitis. They appear to be multifunctional adhesins with affinities to various host substrata, acting to mediate attachment to host surfaces and stimulate immune responses from the colonized host. Here we will review the literature including recent work that has demonstrated the multifaceted nature of AgI/II family proteins, focusing on their overlapping and distinct functions and their important contribution to streptococcal colonization and disease.
Motor Skill Learning-Induced Functional Plasticity in the Primary Somatosensory Cortex: A Comparison Between Young and Older Adults
Authors: Predel Claudia , Kaminski Elisabeth , Hoff Maike , Carius Daniel , Villringer Arno , Ragert Patrick
Published: November 2020

While in young adults (YAs) the underlying neural mechanisms of motor learning are well-studied, studies on the involvement of the somatosensory system during motor skill learning in older adults (OAs) remain sparse. Therefore, the aim of the present study was to investigate motor learning-induced neuroplasticity in the primary somatosensory cortex (S1) in YAs and OAs. Somatosensory evoked potentials (SEPs) were used to quantify somatosensory activation prior and immediately after motor skill learning in 20 right-handed healthy YAs (age range: 19–35 years) and OAs (age range: 57–76 years). Participants underwent a single session of a 30-min co-contraction task of the abductor pollicis brevis (APB) and deltoid muscle. To assess the effect of motor learning, muscle onset asynchrony (MOA) between the onsets of the contractions of both muscles was measured using electromyography monitoring. In both groups, MOA shortened significantly during motor learning, with YAs showing bigger reductions. No changes were found in SEP amplitudes after motor learning in both groups. However, a correlation analysis revealed an association between baseline SEP amplitudes of the N20/P25 and N30 SEP component and the motor learning slope in YAs such that higher amplitudes are related to higher learning. Hence, the present findings suggest that SEP amplitudes might serve as a predictor of individual motor learning success, at least in YAs. Additionally, our results suggest that OAs are still capable of learning complex motor tasks, showing the importance of motor training in higher age to remain an active part of our society as a prevention for care dependency.
Effects of Noise Exposure on the Vestibular System: A Systematic Review
Authors: Stewart Courtney Elaine , Holt Avril Genene , Altschuler Richard A. , Cacace Anthony Thomas , Hall Courtney D. , Murnane Owen D. , King W. Michael , Akin Faith W.
Published: November 2020

Despite our understanding of the impact of noise-induced damage to the auditory system, much less is known about the impact of noise exposure on the vestibular system. In this article, we review the anatomical, physiological, and functional evidence for noise-induced damage to peripheral and central vestibular structures. Morphological studies in several animal models have demonstrated cellular damage throughout the peripheral vestibular system and particularly in the otolith organs; however, there is a paucity of data on the effect of noise exposure on human vestibular end organs. Physiological studies have corroborated morphological studies by demonstrating disruption across vestibular pathways with otolith-mediated pathways impacted more than semicircular canal-mediated pathways. Similar to the temporary threshold shifts observed in the auditory system, physiological studies in animals have suggested a capacity for recovery following noise-induced vestibular damage. Human studies have demonstrated that diminished sacculo-collic responses are related to the severity of noise-induced hearing loss, and dose-dependent vestibular deficits following noise exposure have been corroborated in animal models. Further work is needed to better understand the physiological and functional consequences of noise-induced vestibular impairment in animals and humans.
Prediction of Postoperative Ileus in Patients With Colorectal Cancer by Preoperative Gut Microbiota
Authors: Jin Ye , Geng Rui , Liu Yang , Liu Lujia , Jin Xiangren , Zhao Fuya , Feng Jing , Wei Yunwei
Published: November 2020

Background

Ileus and postoperative ileus (POI) are common complications of colorectal cancer (CRC). However, little is known about the gut microbiota associated with ileus.

Method

Differences in gut microbiota were evaluated by 16S rRNA gene sequencing. We characterized the gut microbiota in 85 CRC patients (cohort 1) and detected differences, and an independent cohort composed of 38 CRC patients (cohort 2) was used to evaluate the results.

Results

The gut microbiota of CRC patients with and without ileus exhibited large differences in alpha- and beta-diversities and bacterial taxa. The Firmicutes-to-Bacteroidetes ratio and microbial dysbiosis index (MDI) showed greater dysbiosis among ileus patients than among those without ileus. According to the location of CRC, the difference in gut microbiota between patients with and without ileus was more obvious in those with distal CRC than in those with proximal CRC. Finally,Faecalibacteriumwas significantly reduced in the postoperative perioperative period in patients with ileus. Thus, we usedFaecalibacteriumas a biomarker for predicting perioperative or POI: the AUC value was 0.74 for perioperative ileus and 0.67 for POI that appeared at 6 months after hospital discharge. The predictive power was evaluated in Cohort 2, with an AUC value of 0.79.

Conclusion

These findings regarding difference of gut microbiota in postoperative CRC patients may provide a theoretical basis for the use of microbiota as biomarkers for the prediction of POI.
PELICAN-IPC 2015-016/Oncodistinct-003: A Prospective, Multicenter, Open-Label, Randomized, Non-Comparative, Phase II Study of Pembrolizumab in Combination With Neo Adjuvant EC-Paclitaxel Regimen in HER2-Negative Inflammatory Breast Cancer
Authors: Bertucci Alexandre , Bertucci François , Zemmour Christophe , Lerebours Florence , Pierga Jean-Yves , Levy Christelle , Dalenc Florence , Grenier Julien , Petit Thierry , Berline Marguerite , Gonçalves Anthony
Published: November 2020

Inflammatory breast cancer (IBC) is a highly aggressive entity with a poor outcome and relative resistance to treatment. Despite progresses achieved during the last decades, the survival remains significantly lower than non-IBC. Recent clinical trials assessing PD-1/PD-L1 inhibitors showed promising results in non-IBC. Pembrolizumab, an anti-PD-1 monoclonal antibody, revolutionized the treatment of different cancers. Several recent studies suggested a potential interest of targeting the immune system in IBC by revealing a more frequent PD-L1 expression and an enriched immune microenvironment when compared with non-IBC. Here, we describe the rationale and design of PELICAN-IPC 2015-016/Oncodistinct-003 trial, an open-label, randomized, non-comparative, phase II study assessing efficacy, and safety of pembrolizumab in combination with anthracycline-containing neoadjuvant chemotherapy in HER2-negative IBC. The trial is ongoing. The primary endpoint is the pCR rate (ypT0/Tis, ypN0) in overall population and the co-primary endpoint is safety profile during a run-in phase. Key secondary objectives include tolerability, invasive disease-free, event-free and overall survivals, as well as collection of tumor and blood samples for translational research.

Clinical Trial Registration

https://clinicaltrials.gov/ (NCT03515798).
One Single Site Clinical Study: To Evaluate the Safety and Efficacy of Immunotherapy With Autologous Dendritic Cells, Cytokine-Induced Killer Cells in Primary Hepatocellular Carcinoma Patients
Authors: Xu Kaiyue , Meng Zhengjie , Mu Xiaoxin , Sun Beicheng , Chai Yi
Published: November 2020

Dendritic cells (DCs) and cytokine-induced killer (CIK) cells play an important role in the anti-tumor immune response. In this study, we evaluated the clinical effectiveness of DC/CIK-CD24 immunotherapies to primary hepatocellular carcinoma patients who received radical resection. 36 resected primary hepatocellular carcinoma (HCC) patients were enrolled from August 2014 to December 2015. All patients received two or four times of DC/CIK immunotherapy after radical resection. 1–4 years patients’ survival rates were evaluated during the follow-up. The 4-year survival rate of patients who received two times of immunotherapy was 47.1%, and the rate of those who received four times of immunotherapies was 52.6%. Compared to baseline, after receiving the DC/CIK-CD24 autotransfusion, the serum Treg concentration of the patients decreased, while CD3+, CD4+, CD56+ increased slightly. The adverse effect of immunotherapy was I–II° transient fever and could be tolerable. DC/CIK-CD24 immunotherapy can delay the relapse time.
Do Current Asthma-Preventive Measures Appropriately Face the World Health Organization's Concerns: A Study Presentation of a New Clinical, Prospective, Multicentric Pediatric Asthma Exacerbation Cohort in Germany
Authors: Aydin Malik , Naumova Ella A. , Lutz Soeren , Meyer-Bahlburg Almut , Arnold Wolfgang H. , Kreppel Florian , Ehrhardt Anja , Postberg Jan , Wirth Stefan
Published: November 2020

In summer 2017, the World Health Organization published 10 facts on asthma, which is known as a major non-communicable disease of high clinical and scientific importance with currently several hundred million people—with many children among them—suffering from air passages inflammation and narrowing. Importantly, the World Health Organization sees asthma as being underdiagnosed and undertreated. Consequently, much more efforts in clinical disease management and research need to be spent on reducing the asthma-related health burden. Particularly, for young approximately 6 months aged patients presenting recurrent bronchitic respiratory symptoms, many parents anxiously ask the doctors for risk prognosis for their children's future life. Therefore, we urgently need to reevaluate if the current diagnostic and treatment measures are in concordance with our yet incomplete knowledge of pathomechanisms on exacerbation. To contribute to this increasing concern worldwide, we established a multicentric pediatric exacerbation study network, still recruiting acute exacerbated asthmatics (children >6 years) and preschool asthmatics/wheezers (children <6 years) since winter 2018 in Germany. The current study that has a currently population comprising 176 study participants aims to discover novel holistic entry points for achieving a better understanding of the poorly understood plasticity of involved molecular pathways and to define biomarkers enabling improved diagnostics and therapeutics. With this study description, we want to present the study design, population, and few ongoing experiments for novel biomarker research.

Clinical Trial Registration: German Clinical Trials Register (Deutsches Register für Klinische Studien, DRKS): DRKS00015738.
Challenges in Monte Carlo Simulations as Clinical and Research Tool in Particle Therapy: A Review
Authors: Muraro S. , Battistoni G. , Kraan A.C.
Published: November 2020

The use and interest in Monte Carlo (MC) techniques in the field of medical physics have been rapidly increasing in the past years. This is the case especially in particle therapy, where accurate simulations of different physics processes in complex patient geometries are crucial for a successful patient treatment and for many related research and development activities. Thanks to the detailed implementation of physics processes in any type of material, to the capability of tracking particles in 3D, and to the possibility of including the most important radiobiological effects, MC simulations have become an essential calculation tool not only for dose calculations but also for many other purposes, like the design and commissioning of novel clinical facilities, shielding and radiation protection, the commissioning of treatment planning systems, and prediction and interpretation of data for range monitoring strategies. MC simulations are starting to be more frequently used in clinical practice, especially in the form of specialized codes oriented to dose calculations that can be performed in short time. The use of general purpose MC codes is instead more devoted to research. Despite the increased use of MC simulations for patient treatments, the existing literature suggests that there are still a number of challenges to be faced in order to increase the accuracy of MC calculations for patient treatments. The goal of this review is to discuss some of these remaining challenges. Undoubtedly, it is a work for which a multidisciplinary approach is required. Here, we try to identify some of the aspects where the community involved in applied nuclear physics, radiation biophysics, and computing development can contribute to find solutions. We have selected four specific challenges: i) the development of models in MC to describe nuclear physics interactions, ii) modeling of radiobiological processes in MC simulations, iii) developments of MC-based treatment planning tools, and iv) developments of fast MC codes. For each of them, we describe the underlying problems, present selected examples of proposed solutions, and try to give recommendations for future research.
Stable and Efficient Agrobacterium-Mediated Genetic Transformation of Larch Using Embryogenic Callus
Authors: Song Yue , Bai Xiaoming , Dong Shiwei , Yang Yuning , Dong Hao , Wang Nairui , Zhang Hanguo , Li Shujuan
Published: November 2020

Larix olgensis or larch is an economically important coniferous tree species with rapid growth in the early stages, strong adaptability, and a short time to harvest. The genetic improvement of larch has garnered considerable attention in recent years for reclaiming timber forests. However, traditional breeding methods are largely ineffective for achieving rapid genetic improvement of L. olgensis. Studies show that the efficiency of plant regeneration can be improved by optimizing somatic embryogenesis. On this basis, we devised a stable, fast and efficient Agrobacterium-mediated genetic transformation method using suspended embryogenic calluses as explants and β-glucuronidase as the reporter. We evaluated the effects of the Agrobacterium load, co-culture period, and addition of acetosyringone and transformant screening antibiotic on the transformation efficiency. In addition, we tested the pCAMBIA 1300-PtHCA 2-1 promoter-GUS binary expression vector, which contains the GUS gene ORF under the control of Populus trichocarpa high cambial activity PtHCA 2-1 promoter, and observed the tissue-specific expression of the GUS gene in the somatic embryos of transgenic larch. This novel technique can not only accelerate the generation of superior transgenic strains of L. olgensis but also aid in future gene functional studies.
Lost in Learning: Hypertext Navigational Efficiency Measures Are Valid for Predicting Learning in Virtual Reality Educational Games
Authors: Ferguson Chris , van Oostendorp Herre
Published: November 2020

The lostness measure, an implicit and unobtrusive measure originally designed for assessing the usability of hypertext systems, could be useful in Virtual Reality (VR) games where players need to find information to complete a task. VR locomotion systems with node-based movement mimic actions for exploration and browsing found in hypertext systems. For that reason, hypertext usability measures, such as “lostness” can be used to identify how disoriented a player is when completing tasks in an educational game by examining steps made by the player. An evaluation of two different lostness measures, global and local lostness, based on two different types of tasks, is described in a VR educational game using 13 college students between 14 and 18 years old in a first study and extended using 12 extra participants in a second study. Multiple Linear Regression analyses showed, in both studies, that local lostness, and not global lostness, had a significant effect on a post-game knowledge test. Therefore, we argued that local lostness was able to predict how well-participants would perform on a post-game knowledge test indicating how well they learned from the game. In-game experience aspects (engagement, cognitive interest, and presence) were also evaluated and, interestingly, it was also found that participants learned less when they felt more present in the game. We believe these two measures relate to cognitive overload, which is known to have an adverse effect on learning. Further research should investigate the lostness measure for use in an online adaptive game system and design the game system in such a way that the risk of cognitive overload is minimized when learning, resulting in higher retention of information.
Social Cognition, Social Skill, and Social Motivation Minimally Predict Social Interaction Outcomes for Autistic and Non-Autistic Adults
Authors: Morrison Kerrianne E. , DeBrabander Kilee M. , Jones Desiree R. , Ackerman Robert A. , Sasson Noah J.
Published: November 2020

Social cognition, social skill, and social motivation have been extensively researched and characterized as atypical in autistic people, with the assumption that each mechanistically contributes to the broader social interaction difficulties that diagnostically define the condition. Despite this assumption, research has not directly assessed whether or how these three social domains contribute to actual real-world social interaction outcomes for autistic people. The current study administered standardized measures of social cognition, social skill, and social motivation to 67 autistic and 58 non-autistic (NA) adults and assessed whether performance on these measures, both individually and relationally between dyadic partners, predicted outcomes for autistic and NA adults interacting with unfamiliar autistic and NA partners in a 5 minute unstructured “get to know you” conversation. Consistent with previous research, autistic adults scored lower than NA adults on the three social domains and were evaluated less favorably by their conversation partners. However, links between autistic adults' performance on the three social domains and their social interaction outcomes were minimal and, contrary to prediction, only the social abilities of NA adults predicted some interaction outcomes within mixed diagnostic dyads. Collectively, results suggest that reduced performance by autistic adults on standardized measures of social cognition, social skill, and social motivation do not correspond in clear and predictable ways with their real-world social interaction outcomes. They also highlight the need for the development and validation of more ecological assessments of autistic social abilities and the consideration of relational dynamics, not just individual characteristics, when assessing social disability in autism.
Perception of Cyberbullying in Adolescence: A Brief Evaluation Among Italian Students
Authors: Saladino Valeria , Eleuteri Stefano , Verrastro Valeria , Petruccelli Filippo
Published: November 2020

Cyberbullying is associated with the expansion of digital devices and the Internet. In Italy and other European and non-European countries, the phenomenon is growing. Young people who suffer from cyberbullying develop psychopathological symptoms of anxiety, depression, and social phobia that can lead to extreme acts, such as suicide. The pressure, the sense of isolation, and helplessness experienced by cyber-victims also affect their family and the school context. Cyberbullying is acted through digital tools, it is often anonymous, and aims to destroy and psychologically humiliate the victim. There are various forms of cyberbullying that involve different reactions and consequences. However, few studies have focused on adolescents’ perception of cyberbullying. Youths often engage in aggressive behaviors, ignoring the feelings and reactions of the victims. Based on these considerations, our article aims to provide a general overview of the spread of the phenomenon and to understand the various types of cyberbullying and its consequences on victims. We will also illustrate a brief evaluation conducted in Italian schools investigating the perception of cyberbullying in a sample of 600 Italian adolescents (11–14 years old). Our work aims to investigate the cognition and the personal perception of youths about cyberbullying and its consequences and to promote educational interventions within and outside the context of school.
Evaluation of the Agronomic Traits of 80 Accessions of Proso Millet (Panicum miliaceum L.) under Mediterranean Pedoclimatic Conditions
Authors: Calamai Alessandro , Masoni Alberto , Marini Lorenzo , Dell’acqua Matteo , Ganugi Paola , Boukail Sameh , Benedettelli Stefano , Palchetti Enrico
Published: November 2020

The continuous increase in the world population and the associated food demands in the wake of climate change are pushing for the development and cultivation of climate-resilient crops that are able to efficiently use natural resources. Proso millet (Panicum miliaceum L.) might be a promising candidate crop thanks to its heat stress resistance and its limited water demand. To date, one of the most important strategies to increase grain yield and to improve other agronomic important traits is through an efficient breeding program based on a wide genetic variability of parental germplasm. In this study, we evaluated the agronomical traits of a world collection of 80 P. miliaceum accessions. The entire collection was evaluated over a 2 year field experiment under Mediterranean pedoclimatic conditions, which exhibited a wide range of variability for plant height (25–111 cm), grain yield (842–3125 kg ha−1), total dry biomass (2767–10,627 kg ha−1), harvest index (HI; 0.25–0.35), Growing Degree Days (GDDs; 581–899), and days to maturity (80–111 d). A non-parametric multivariate analysis of variance (Np-MANOVA) analysis indicated that GDDs to flowering, grain yield, total dry biomass, days to maturity, plant height, and seed yield per plant were useful parameters to differentiate the germplasm accessions. High heritability (>0.60) was observed in both years for plant height, leaf number, basal tiller, seed yield per plant, 100-seed weight, GDDs to flowering, and days to maturity. Grain yield, total dry biomass, and HI reported moderate heritability (0.30–0.60). The findings reported in the present study may provide valuable information that could support researchers in breeding programs to develop high grain-yielding accessions.
Italian Zeolitites in the Control of Grey Mould and Sour Rot and Their Effect on Leaf Reflectance, Grape and Wine
Authors: Calzarano Francesco , Seghetti Leonardo , Pagnani Giancarlo , Di Marco Stefano
Published: November 2020

The activity of two zeolitites, differing in the presence of copper and their particle size, was assessed in a Trebbiano d’Abruzzo white berry cultivar, towards grapevine grey mould and sour rot, a serious problem in almost all grapevine-growing areas. In 2018 and 2019, zeolitites were applied before forecast of rainfalls, which likely induce infection. In 2019, further applications were carried out 10 or 2 days before harvest, regardless of the rainfall, in order to verify the effect of the zeolitite residue on leaf reflectance and composition of grapes and wines. Both zeolitites significantly reduced the infections. Only the vines treated 2 days before harvest, especially with copper zeolitite, showed a decrease in leaf reflectance. However, no differences in the yield composition related to the time of zeolitite application, as well as a decrease of yield quality, were ever recorded. Furthermore, analysis of samples collected from vines treated with zeolitites showed increases in grape soluble solids and in wine alcohol content. Our results suggest the use of zeolitites against grapevine rots as a possible effective alternative to the application of synthetic fungicides in the development of environmentally friendly control strategies.
Is Drought Stress Tolerance Affected by Biotypes and Seed Size in the Emerging Oilseed Crop Camelina?
Authors: Čanak Petar , Jeromela Ana Marjanović , Vujošević Bojana , Kiprovski Biljana , Mitrović Bojan , Alberghini Barbara , Facciolla Erika , Monti Andrea , Zanetti Federica
Published: November 2020

One of the main advantages of camelina (Camelina sativa (L.) Crantz) is its wide environmental adaptability and extreme drought tolerance. The availability of both winter and spring camelina biotypes, characterized by different seed sizes, raises the question about possible differences in their response to drought stress at the emergence stage. To address this, a germination test was set up in controlled conditions, comparing six winter and six spring genotypes with differing seed sizes (ranging from 1.83 to 0.88 g/1000-seeds) under increasing levels of osmotic stress (0, −0.4, −0.8, −1.2, −1.4, −1.6 MPa) using polyethylene glycol (PEG). Camelina withstands mild level of osmotic stress (−0.4 MPa) without significant decrease in germination. Even at −1.2 MPa after 10 d, it still had 75% germination. Significant differences in germination were observed between biotypes, where spring biotypes performed better than winter ones. Shoot and radicle lengths were significantly diminished by imposed osmotic stress, but shoot growth seemed more impacted. In general, spring biotypes had longer shoots and radicles than winter ones. Seed size played a role in the response of camelina to drought, but it depended on biotype and stress level imposed. In particular large seeded spring types had the highest germination percentage and resulted less impaired by osmotic stress, otherwise among the tested winter types the small seeded ones were the best performing. The presented data could be useful for breeding purposes for selecting the appropriate camelina type for sowing in drought-prone regions.
In Vitro Antibacterial Activity of Manuka (Leptospermum scoparium J.R. et G. Forst) and winter Savory (Satureja montana L.) Essential Oils and Their Blends against Pathogenic E. coli Isolates from Pigs
Authors: Fratini Filippo , Forzan Mario , Turchi Barbara , Mancini Simone , Alcamo Giuseppe , Pedonese Francesca , Pistelli Luisa , Najar Basma , Mazzei Maurizio
Published: November 2020

Neonatal diarrhoea (ND), post-weaning diarrhoea (PWD) and oedema disease (OD) are among the most important diseases affecting pig farming due to economic losses. Among the main aetiological agents, strains of Escherichia coli are identified as the major responsible pathogens involved. Several strategies have been put in place to prevent these infections and, today, research is increasingly studying alternative methods to antibiotics to reduce the antibiotic resistance phenomenon. Essential oils (EOs) are among the alternative tools that are being investigated. In this study, the in vitro effectiveness of winter savory and manuka essential oils and their mixtures in different proportions against strains of E. coli isolated from episodes of disease in pigs was evaluated. The EOs alone demonstrated slight antibacterial effectiveness, whereas the blends, by virtue of their synergistic action, showed remarkable activity, especially the 70%–30% winter savory–manuka blend, showing itself as a potential tool for prevention and therapy.
Hawthorn Herbal Preparation from Crataegus oxyacantha Attenuates In Vivo Carbon Tetrachloride -Induced Hepatic Fibrosis via Modulating Oxidative Stress and Inflammation
Authors: Hamza Alaaeldin Ahmed , Lashin Fawzy Mohamed , Gamel Mona , Hassanin Soha Osama , Abdalla Youssef , Amin Amr
Published: November 2020

Hawthorn (HAW) is a herbal preparation extracted from Crataegus oxyacantha. HAW has cardioprotective, antioxidants, anti-inflammatory, and anti-hypotensive effects. HAW’s effect on hepatic fibrosis remains, however, unknown. This study evaluated the impact of HAW on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats and elucidated its mechanisms. HAW reduced liver index and the serum liver enzyme markers and reduced liver damage, and fibrosis as confirmed by histopathological scoring of hematoxylin-eosin staining. Collagen deposition was reduced in HAW group compared to CCl4 group as confirmed by Masson staining, hydroxyproline content, and both mRNA and protein levels of alpha-smooth muscle actin, collagen 1 and 3. HAW also down regulated the gene expressions of inflammatory markers including interleukin-IL-1β, tumor necrosis factor-α, transforming growth factor-β 1, nuclear factor kappa-B, and cyclooxygenase-2 and decreased the myeloperoxidase activity. The effects of HAW was also associated with decreased levels of hepatic oxidative stress markers (malondialdehyde and P.Carbonyl) and with increased activity of superoxide dismutase. Those effects are possibly mediated by blocking the pro-oxidant machinery and down regulating the inflammatory and profibrotic responses. Finally, chlorogenic acid, epicatechin, rutin, vitexin quercetin, and iso quercetin were identified as the major species of polyphenols of the HAW herbal preparation used here. Therefore, HAW’s potent protecting effects against liver fibrosis predicts a significant beneficial application.
Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism
Authors: Janse van Rensburg Henry Christopher , Van den Ende Wim
Published: November 2020

The stress-inducible non-proteinogenic amino acid γ-aminobutyric acid (GABA) is known to alleviate several (a)biotic stresses in plants. GABA forms an important link between carbon and nitrogen metabolism and has been proposed as a signalling molecule in plants. Here, we set out to establish GABA as a priming compound against Botrytis cinerea in Arabidopsis thaliana and how metabolism and reactive oxygen species (ROS) are influenced after GABA treatment and infection. We show that GABA already primes disease resistance at low concentrations (100 µM), comparable to the well-characterized priming agent β-Aminobutyric acid (BABA). Treatment with GABA reduced ROS burst in response to flg22 (bacterial peptide derived from flagellum) and oligogalacturonides (OGs). Plants treated with GABA showed reduced H2O2 accumulation after infection due to increased activity of catalase and guaiacol peroxidase. Contrary to 100 µM GABA treatments, 1 mM exogenous GABA induced endogenous GABA before and after infection. Strikingly, 1 mM GABA promoted total and active nitrate reductase activity whereas 100 µM inhibited active nitrate reductase. Sucrose accumulated after GABA treatment, whereas glucose and fructose only accumulated in treated plants after infection. We propose that extracellular GABA signalling and endogenous metabolism can be separated at low exogenous concentrations.
Digital Twins for Additive Manufacturing: A State-of-the-Art Review
Authors: Zhang Li , Chen Xiaoqi , Zhou Wei , Cheng Taobo , Chen Lijia , Guo Zhen , Han Bing , Lu Longxing
Published: November 2020

With the development of Industry 4.0, additive manufacturing will be widely used to produce customized components. However, it is rather time-consuming and expensive to produce components with sound structure and good mechanical properties using additive manufacturing by a trial-and-error approach. To obtain optimal process conditions, numerous experiments are needed to optimize the process variables within given machines and processes. Digital twins (DT) are defined as a digital representation of a production system or service or just an active unique product characterized by certain properties or conditions. They are the potential solution to assist in overcoming many issues in additive manufacturing, in order to improve part quality and shorten the time to qualify products. The DT system could be very helpful to understand, analyze and improve the product, service system or production. However, the development of genuine DT is still impeded due to lots of factors, such as the lack of a thorough understanding of the DT concept, framework, and development methods. Moreover, the linkage between existing brownfield systems and their data are under development. This paper aims to summarize the current status and issues in DT for additive manufacturing, in order to provide more references for subsequent research on DT systems.
Effect of Soil-Structure Interaction on the Seismic Response of Existing Low and Mid-Rise RC Buildings
Authors: Oz Ibrahim , Senel Sevket Murat , Palanci Mehmet , Kalkan Ali
Published: November 2020

Reconnaissance studies performed after destructive earthquakes have shown that seismic performance of existing buildings, especially constructed on weak soils, is significantly low. This situation implies the negative effects of soil-structure interaction on the seismic performance of buildings. In order to investigate these effects, 40 existing buildings from Turkey were selected and nonlinear models were constructed by considering fixed-base and stiff, moderate and soft soil conditions. Buildings designed before and after Turkish Earthquake code of 1998 were grouped as old and new buildings, respectively. Different soil conditions classified according to shear wave velocities were reflected by using substructure method. Inelastic deformation demands were obtained by using nonlinear time history analysis and 20 real acceleration records selected from major earthquakes were used. The results have shown that soil-structure interaction, especially in soft soil cases, significantly affects the seismic response of old buildings. The most significant increase in drift demands occurred in first stories and the results corresponding to fixed-base, stiff and moderate cases are closer to each other with respect to soft soil cases. Distribution of results has indicated that effect of soil-structure interaction on the seismic performance of new buildings is limited with respect to old buildings.
Study on the Applicability of Dynamic Factor Standards by Comparison of Spring Constant Based Dynamic Factor of Ballasted and Concrete Track Structures
Authors: Lee Jaeik , Oh Kyuhwan , Park Yonggul , Choi Junhyeok
Published: November 2020

Dynamic factor evaluation method calculation methods outlined by Eisenmann (DAFEisenmann) and the American Railway Engineering Association (DAFArea) are used to calculate the dynamic factor during design and for trackside measurement, respectively, in nations where the construction of concrete track structures is relatively new. In this situation, dynamic factor calculation methods may be incorrect, and this is demonstrated by comparison of the respective track types’ total spring constant. A finite element analysis of a standard design railway track is conducted, and the design total spring constant (TSC, or K) obtained from the time history function analysis is compared to the TSC of existing tracks through trackside measurement results. The comparison result shows that TSC obtained by finite element analysis result is 22% higher than that of the trackside measurement value, indicating that the TSC is conservative in the current track design. Considering the proportional relationship between TSC and dynamic factor, it is estimated that the dynamic factor currently being applied in track design is also conservative. Based on these findings, an assessment of the applicability of different dynamic factors (DAFEisenmann and DAFArea), theoretical calculation and field measurement (DAFField) using the probabilistic analysis of wheel loads from the field measurement data is conducted. A correlative analysis between DAFEisenmann and DAFArea shows that DAFEisenmann and DAFArea were estimated to be higher by 33% and 27% in ballasted track and by 39% and 30% in concrete track than the dynamic factor derived from field measurement, respectively, which indicates that the dynamic factor currently in use can potentially lead to over-estimation in track design and maintenance.
A Study on the Influence of the Cross-Sectional Shape of the Metal-Inserted Retainer Ring and the Pressure Distribution from the Multi-Zone Carrier Head to Increase the Wafer Yield
Authors: Park Joon-Yeon , Han Je-Heon , Kim Changwon
Published: November 2020

In this paper, for the purpose of increasing the wafer yield by controlling the non-uniformity of the material removal rate during the chemical mechanical polishing process, the influence of the cross-sectional shape of the metal-inserted retainer ring and the pressure distribution on the wafer and the retainer ring generated from the multi-zone carrier head are investigated. First, in order to verify the finite element analysis model, it is correlated using the test data. By using a validated finite element model, simulation studies involving several parameters are performed to reduce the irregularity in the wafer: (1) tapered bottom of the retainer ring, (2) machining round corners at the bottom of the retainer ring, (3) the changes in pressure applied to the wafer, (4) the changes in pressure applied to the retainer ring.
Optimal Control as a Tool for Innovation in Aerial Twisting on a Trampoline
Authors: Charbonneau Eve , Bailly François , Danès Loane , Begon Mickaël
Published: November 2020

Aerial twisting techniques are preferred by trampoline coaches for their balanced landings. As these techniques are not intuitive, computer simulation has been a relevant tool to explore a variety of techniques. Up to now, twisting somersaults were mainly simulated using arm abduction/adduction only (2D). Our objective was to explore more complex (3D) but still anatomically feasible arm techniques to find innovative and robust twisting techniques. The twist rotation was maximized in a straight backward somersault performed by a model including arm abduction/adduction with and without changes in the plane of elevation. A multi-start approach was used to find a series of locally optimal performances. Six of them were retained and their robustness was assessed by adding noise to the first half of the arm kinematics and then reoptimizing the second half of the skill. We found that aerial twist performance linearly correlates with the complexity of arm trajectory. Optimal techniques share a common strategy consisting of moving the arm in a plane formed by the twisting and angular momentum axes, termed as the best tilting plane. Overall, 3D techniques are simpler and require less effort than 2D techniques for similar twist performances. Three techniques which generate ∼3 aerial twists could be used by athletes because kinematic perturbations do not compromise the performance and the landing.