bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Classification of Large B-Cell Lymphoma and High-Grade B-Cell Lymphoma Cases and Association with Outcomes in Morocco
Authors: Taybi Mahat , Khammar Zineb , Alami Drideb Noufissa , Berrady Rhizlane , Benmiloud Sanae , Bouguennouch Laila , Elfakir Sanae , Tahiri Laila , Majdoub Mohammed , Chbani Laila , Hammas Nawal
Published: October 2024

Background: High-grade B-cell lymphoma with c-MYC and BCL2 and/or BCL6 rearrangements (HGBL-DHL/THL) is a recently identified category in the most recent World Health Organization (WHO) classification. For all tumors displaying the appearance of diffuse large B-cell lymphoma (DLBCL) or high-grade B-cell lymphoma (HGBL), it is necessary to perform fluorescence in situ hybridization (FISH) in order to achieve an accurate diagnosis. The findings of FISH and immunohistochemistry (IHC) examinations from 50 DLBCL/HGBL samples obtained from Hassan II University Hospital in Fez/Morocco are reported. Methods: This retrospective study included 50 patients diagnosed with DLBCL/HGBL over a period of nine years (2013–2022) and treated with RCHOP chemotherapy protocol. All patients underwent a histological study followed by an immunohistochemical study to confirm the diagnosis and to classify patients according to cell of origin into non-GCB and GCB subtypes; then, a cytogenetic study using FISH was performed to classify patients according to the presence or absence of rearrangements in the c-MYC, BCL2 and BCL6 genes. A comparison was made between the molecular subtypes of DLBCL/HGBL in relation to clinicopathological features and outcomes. Results: Among the 50 cases studied in our population, we found 5 cases of HGBL with DLBCL morphology and 45 cases of DLBCL, which consisted of 13 cases (28.89%) of GCB subtype and 32 cases (71.11%) of non-GCB subtype based on the immunohistochemistry Hans algorithm. After FISH testing of all cases, we found three cases of double-hit lymphoma (DHL) and one case of triple-hit lymphoma (THL). Thus, HGBL-DHL/THL accounted for 8% of the cases. Furthermore, two cases were detected with only one rearrangement in the BCL2 gene and one case harboring a rearrangement in the BCL6 gene. DHL and THL patients and patients with a single rearrangement (BCL2 or BCL6) have a worse prognosis than patients with no rearrangement. Conclusions: DHL and THL are an aggressive entity of HGBL with poorer outcomes in comparison to DLBCL/HGBL NOS. First-line treatment with the RCHOP chemotherapy protocol may not be effective for all aggressive DLBCL cases. More targeted treatment is crucial for better patient outcomes.
Genome-Wide Identification and Expression Analysis of the MADS-Box Gene Family in Cassava (Manihot esculenta)
Authors: Zhang Qin , Li Yanan , Geng Sha , Liu Qian , Zhou Yingchun , Shen Shaobin , Shen Zhengsong , Ma Dongxiao , Xiao Mingkun , Luo Xin , Che Bin , Li Kang , Yan Wei
Published: October 2024

The MADS-box gene family constitutes a vital group of transcription factors that play significant roles in regulating plant growth, development, and signal transduction processes. However, research on the MADS-box genes in cassava (Manihot esculenta) has been relatively limited. To gain deeper insights into the functions of the MADS-box genes in cassava development, in this study, we undertook a comprehensive genome-wide identification of the MADS-box gene family in cassava. We identified a total of 86 MADS-box genes with complete domains in the cassava genome, designated as MeMADS01 to MeMADS86. Through bioinformatic analyses, we investigated the basic physicochemical properties, conserved motifs, chromosomal locations, and phylogenetic relationships of the cassava MADS-box genes. The MADS-box gene family of cassava exhibited conservation, as well as species-specific characteristics, with intron loss being a predominant mode of evolution for the MADS-box genes. Expression pattern variations in the MeMADS genes across different tissues offer insights into their potential functions. Time-ordered gene co-expression network (TO-GCN), transcriptome data, and RT-qPCR analysis suggested the responsiveness of the MADS-box genes to drought stress. Meanwhile, MeMADS12 might be involved in regulating flowering under drought conditions via an ABA (abscisic acid)-dependent pathway. These findings provide valuable resources for a deeper understanding of the biological roles of the MADS-box genes in cassava.
Black Clinicians’ Perceptions of the Cultural Relevance of Parent–Child Interaction Therapy for Black Families
Authors: Coates Erica E. , Coffey Sierra , Farrise Beauvoir Kaela , Aron Emily , Hayes Katherine R. , Chavez Felipa T.
Published: October 2024

Parent–child interaction therapy (PCIT) is a highly efficacious, evidence-based treatment for children with disruptive behaviors and their families. PCIT is a dyadic therapy designed to improve parent–child relationships and decrease children’s behavioral problems. PCIT research specific to Black families is currently sparse. Given findings that Black families have a higher attrition rate and demonstrate fewer significant improvements in parental well-being outcomes, we sought to assess clinicians’ perceived cultural alignment of PCIT with Black families. We conducted individual interviews via Zoom with 10 Black clinicians, trained in PCIT, who had experience treating Black families using PCIT. The research team generated the following themes using thematic analysis: cultural misalignment, manualization, barriers to treatment, generational patterns of discipline, racial considerations, and protocol changes. Findings indicate that Black clinicians have identified various points of cultural misalignment in providing PCIT with Black families, for which they have modified treatment or suggested changes to improve cultural sensitivity. Collating suggested clinician modifications to inform a cultural adaptation of PCIT for Black families may contribute to a reduction in the attrition rate and improvement in outcomes for Black families participating in PCIT.
Stakeholder Perspectives on the Acceptability, Design, and Integration of Produce Prescriptions for People with Type 2 Diabetes in Australia: A Formative Study
Authors: Law Kristy , Trieu Kathy , Madz Jennifer , Coyle Daisy , Glover Kimberly , Tian Maoyi , Xin Yuze , Simmons David , Wong Jencia , Wu Jason
Published: October 2024

Produce prescription programs can benefit both individuals and health systems; however, best practices for integrating such programs into the Australian health system are yet unknown. This study explored stakeholders’ perspectives on the acceptability, potential design and integration of produce prescription programs for adults with type 2 diabetes in Australia. Purposive sampling was used to recruit 22 participants for an online workshop, representing six stakeholder groups (government, healthcare service, clinician, food retailer, consumer, non-government organisation). Participant responses were gathered through workshop discussions and a virtual collaboration tool (Mural). The workshop was video-recorded and transcribed verbatim, and thematic analysis was conducted using a deductive–inductive approach. Stakeholders recognised produce prescription as an acceptable intervention; however, they identified challenges to implementation related to contextuality, accessibility, and sustainability. Stakeholders were vocal about the approach (e.g., community-led) and infrastructure (e.g., screening tools) needed to support program design and implementation but expressed diverse views about potential funding models, indicating a need for further investigation. Aligning evaluation outcomes with existing measures in local, State and Federal initiatives was recommended, and entry points for integration were identified within and outside of the Australian health sector. Our findings provide clear considerations for future produce prescription interventions for people with type 2 diabetes.
Comprehensive Assessment of Large-Scale Regional Fluvial Flood Exposure Using Public Datasets: A Case Study from China
Authors: Chen Xuanchi , Liang Bingjie , Li Junhua , Cai Yingchun , Liang Qiuhua
Published: October 2024

China’s vulnerability to fluvial floods necessitates extensive exposure studies. Previous large-scale regional analyses often relied on a limited set of assessment indicators due to challenges in data acquisition, compounded by the scarcity of corresponding large-scale flood distribution data. The integration of public datasets offers a potential solution to these challenges. In this study, we obtained four key exposure indicators—population, built-up area (BA), road length (RL), and average gross domestic product (GDP)—and conducted an innovative analysis of their correlations both overall and locally. Utilising these indicators, we developed a comprehensive exposure index employing entropy-weighting and k-means clustering methods and assessed fluvial flood exposure across multiple return periods using fluvial flood maps. The datasets used for these indicators, as well as the flood maps, are primarily derived from remote sensing products. Our findings indicate a weak correlation between the various indicators at both global and local scales, underscoring the limitations of using singular indicators for a thorough exposure assessment. Notably, we observed a significant concentration of exposure and river flooding east of the Hu Line, particularly within the eastern coastal region. As flood return periods extended from 10 to 500 years, the extent of areas with flood depths exceeding 1 m expanded markedly, encompassing 2.24% of China’s territory. This expansion heightened flood risks across 15 administrative regions with varying exposure levels, particularly in Jiangsu (JS) and Shanghai (SH). This research provides a robust framework for understanding flood risk dynamics, advocating for resource allocation towards prevention and control in high-exposure, high-flood areas. Our findings establish a solid scientific foundation for effectively mitigating river flood risks in China and promoting sustainable development.
The Value of Reducing Inconclusive and False-Positive Newborn Screening Results for Congenital Hypothyroidism, Congenital Adrenal Hyperplasia and Maple Syrup Urine Disease in The Netherlands
Authors: Martens Rosalie C. , Boelen Anita , van der Kemp Michèle H. , Bosch Annet M. , Berghout Eveline M. , Weijman Gert , Zwaveling-Soonawala Nitash , Verschoof-Puite Rendelien K. , de Jonge Robert , Hannema Sabine E. , Bosmans Judith E. , Heijboer Annemieke C.
Published: October 2024

Inconclusive and false-positive newborn screening (NBS) results can cause parental stress and increase healthcare expenditures. These results can be reduced by improving NBS algorithms. This was recently done for Congenital Hypothyroidism (CH), Congenital Adrenal Hyperplasia (CAH) and Maple Syrup Urine Disease (MSUD) in the Dutch NBS program. The current study estimates the financial consequences of these improved algorithms related to the reduction in inconclusive results and false-positives. For each improved algorithm, the care pathway of an inconclusive/false-positive result was analyzed. The costs associated with the improvements, based on the change in inconclusive results/false-positives, were assessed to estimate the cost reduction per year. The improvements resulted in a reduction of inconclusive results and/or false-positives, without increasing false-negatives. For CH, false positives decreased by 26 per year with a related cost reduction of EUR 31,156. For CAH, 95 second heel punctures and seven false-positives per year were avoided, leading to a related cost reduction of EUR 7340. For MSUD, five false-positives per year were avoided with a related cost reduction of EUR 11,336. The improved screening algorithms led to a cost reduction of EUR 49,832 annually. Together with the known negative psychosocial effects associated with an inconclusive or false-positive NBS result, these results highlight the importance of improving NBS algorithms.
Anion and Cation Dynamics in Mixed-Anion Hydroborate Na3(BH4)(B12H12): 1H, 11B, and 23Na NMR Studies
Authors: Babanova Olga A. , Sadikin Yolanda , Skoryunov Roman V. , Soloninin Alexei V. , Skripov Alexander V.
Published: October 2024

Sodium borohydride-closo-hydroborate Na3(BH4)(B12H12) exhibits high room-temperature ionic conductivity and high electrochemical stability. To study the dynamical properties of this mixed-anion compound at the microscopic level, we have measured the 1H, 11B, and 23Na nuclear magnetic resonance spectra and nuclear spin-lattice relaxation rates over the temperature range of 8–573 K. Our 1H and 11B spin-lattice relaxation measurements have revealed two types of reorientational jump motion. The faster motional process attributed to reorientations of the [BH4]− anions is characterized by an activation energy of 159 meV, and the corresponding reorientational jump rate reaches ~108 s−1 near 130 K. The slower process ascribed to reorientations of the larger [B12H12]− anions is characterized by an activation energy of 319 meV, and the corresponding reorientational jump rate reaches ~108 s−1 near 240 K. The results of the 23Na nuclear magnetic resonance measurements are consistent with the fast long-range diffusion of Na+ ions in Na3(BH4)(B12H12). The diffusive jump rate of Na+ is found to reach ~104 s−1 at 300 K and ~8 × 108 s−1 at 530 K. A comparison of these jump rates with the ionic conductivity data suggests the importance of correlations between diffusing ions.
GIS-Enhanced Survey of Potential Aedes aegypti and Aedes albopictus Artificial Oviposition Containers Distributed across Communities in Trinidad, West Indies
Authors: Hapairai Limb K. , Seeramsingh Roshan , James Lester D. , Feng Rachel S. , Nandram Naresh , Mohammed Azad , Duman-Scheel Molly , Severson David W.
Published: October 2024

Dengue and other arboviruses remain a global threat, and enhanced efforts to control the mosquitoes that transmit them are urgently needed. A survey of potential manmade Aedes aegypti (L.) and Aedes albopictus (Skuse) oviposition containers was performed in four communities near the end of the typical dry season in 2018 in Trinidad, West Indies. The purpose was to conduct individual premise surveys and use GIS mapping to visualize premises within communities that had Aedes-positive containers, as this information could be used for the prioritization of mosquito control efforts in potential high risk areas as the wet season progressed. Accessible premises were surveyed following standard inspection protocols used by the Insect Vector Control Division (IVCD), Ministry of Health (MOH). The results indicated that two of the four locations would be at high risk for arbovirus transmission going into the wet season. The GIS mapping of premises with Aedes-positive containers facilitated the identification of potential hot spots for arbovirus transmission risk within communities that should be prioritized for enhanced monitoring and vector control efforts, emphasizing the need to increase community participation in standard surveys by IVCD.
Spontaneous Color Preferences and Associative Learning in Protaetia brevitarsis (Coleoptera: Scarabaeidae)
Authors: Wu Hui , Cui Zhuangzhi , Huang Xiaoqing , Dhiloo Khalid , Kong Fanfang , Wang Zhongyue , Liu Yongqiang
Published: October 2024

Color vision, which varies among species, plays an important role in foraging, mating, and habitat selection among insects. Protaetia brevitarsis (Coleoptera: Scarabaeidae, Lewis) is an omnivorous beetle that damages both crops and fruit. Here, to understand the effect of vision and olfaction in host selection, experiments were conducted on the spectral wavelength preference, color preference, and associative learning ability of adult P. brevitarsis using LED lights and grapes. In our experiments, adults showed the strongest spontaneous preference toward the red spectrum, particularly 730 nm. Non-preferred lights were used to train adults with a food reward (grapes). Green-trained adults had an increasing tendency to prefer green light, and blue-trained adults had a clear preference for blue light. Furthermore, adults significantly preferred red grapes in the absence of olfactory cues, but their selectivity for grapes differed in the presence of olfactory cues, indicating that vision was not the only factor in foraging decisions, but that olfactory cues also influenced their decision making. The results lay the groundwork for revealing their host localization mechanism and provide promising avenues for biological control in the field.
Safety of Magnetic Resonance Imaging in Patients with Cardiac Implantable Electronic Devices
Authors: Lanz Hugo , Strauß Katharina , Höpler Julia , Kraft Marie , Hoffmann Sabine , Binzenhöfer Leonhard , Gade Nils , Roden Daniel , Saleh Inas , Kääb Stefan , Lackermair Korbinian , Sadoni Sebastian , Hagl Christian , Massberg Steffen , Estner Heidi , Fichtner Stephanie , Lüsebrink Enzo
Published: October 2024

Background: MRI (magnetic resonance imaging) represents the diagnostic image modality of choice in several conditions. With an increasing number of patients requiring MRI for diagnostic purposes, the issue of safety in patients with cardiac implantable electronic devices (CIED) undergoing this imaging modality will play an ever more important role. The purpose of this study was to assess the safety and device function following MRI in an unrestricted real-world cohort of patients with a wide array of cardiac devices. Methods: We conducted a retrospective single-center study including 1010 MRI studies conducted in adult patients (≥18 years) with an implanted CIED treated in the University Hospital of Munich (LMU) between July 2012 and March 2024. Patients with non-MR conditionally labeled leads, abandoned or epicardial leads, as well as lead fragments, were included for analysis. Results: Across a total of 1010 MRIs (920 total MR-conditional device generators) performed in patients with an implanted CIED, there were no deaths, reports of discomfort, palpitations, heating, or ventricular arrythmias in the 24 h following MRI. Only 2/1010 MRIs were followed by a reported atrial arrhythmia within 24 h, both in patients with an MR-conditional pacemaker (PM) device without an abandoned lead. No significant changes in device function following MRI from baseline were observed across all included CIEDs. Lastly, no instances of severe malfunction, such as generator failure, loss of capture, electrical reset, or inappropriate inhibition of pacing, were found in post-MRI interrogation reports across all MRI studies. Conclusions: Based on the analysis of 1010 MRIs undergone by patients with CIEDs, following standardized device interrogation, manufacturer-advised device programming, monitoring of vital function, and manufacturer-advised reprogramming, MRI can be performed safely and without adverse events or changes in device function.
Auditory Rehabilitation in Single-Sided-Deafened Patients after Surgery to the Cerebellopontine Angle for Vestibular Schwannoma: What Is the Patient’s Choice?
Authors: Loukine Bézé Margaux , Puechmaille Mathilde , Trillat Chloé , Barrat Antoine , Bécaud Justine , Saroul Nicolas , Khalil Toufic , Coll Guillaume , Mom Thierry
Published: October 2024

Background: Surgical resection of vestibular schwannomas (VS) can be responsible for single-sided deafness (SSD). Hearing restoration can be a challenge both for the otolaryngologist and the patient. Patients and Methods: In a retrospective series, we analyzed the charts of SSD patients operated on for VS from 2005–2021, checking which type of hearing rehabilitation was chosen. All patients who wanted a hearing restoration underwent a hearing in noise test (HINT) in a stereo auditorium with and without a bone-anchored hearing device (BAHD) worn with a headband on the deaf side. Then, they had a preimplantation one-month trial with the BAHD at home vs. contralateral routing of signal (CROS) or BiCROS (with contralateral signal amplification) hearing aids (HAs). Results: Among 52 charts of the included adult SSD patients, only 29 (56%) eventually chose a hearing rehabilitation device (14 BAHD). Only one BAHD patient required a device explantation for skin complications, but then asked for reimplantation. Another one swapped the BAHD for HAs 2.5 years after. Two patients only occasionally used their BAHD with a headband. Nine patients preferred HAs, mainly BiCROS. Their contralateral hearing was significantly less than BAHD patients (p < 0.05), and only three used their HAs every day. Conclusions: Hearing rehabilitation in SSD patients after VS surgical resection is chosen in about 50% of cases. In complement of HINT, a real-life comparative hearing trial helps patients chose the best device, with good long-term results when a BAHD is chosen. HAs are preferred when contralateral hearing is altered but are not always worn.
Exaggerated Blood Pressure Response to Exercise Is a Risk of Future Hypertension Even in Healthy, Normotensive Young Individuals—Potential Preventive Strategies for This Phenomenon?
Authors: Kunimatsu Narumi , Tsukamoto Hayato , Ogoh Shigehiko
Published: October 2024

Physical activity and regular exercise are well known to reduce the risks of cerebrovascular and cardiovascular diseases, leading the American College of Sports Medicine to endorse the concept that “exercise is medicine”. However, a single bout of exercise temporarily raises arterial blood pressure (BP) to meet the metabolic demands of working muscle, and this BP response is particularly exaggerated in older adults and patients with cardiovascular conditions, such as hypertension, resulting in an exaggerated BP response during exercise. This presents a paradox: while regular exercise is crucial for preventing these diseases, excessively high BP responses during exercise could increase the risk of vascular damage. The mechanisms underlying this exaggerated BP response during exercise remain unclear, and effective exercise regimens for these populations have yet to be established. Currently, low-intensity exercise is recommended; however, its efficacy in disease prevention is uncertain. Notably, even among healthy individuals, there is significant variation in the BP response to exercise. Some healthy individuals, despite having normal resting BP, exhibit an exaggerated BP response during physical activity. Importantly, these individuals are often unaware that their BP becomes excessively elevated during physical activity. Repeated exposure to these heightened BP responses through regular physical activity may increase their long-term risk of cardiovascular disease. How can we prevent disease development in these individuals while still ensuring the effectiveness of exercise? Some studies have shown that individuals with a family history of hypertension may experience this phenomenon even in children and adolescents. Additionally, left ventricular hypertrophy contributes to an exaggerated BP response to exercise, suggesting a possible genetic influence. Conversely, other reports indicate that factors such as arterial stiffness, obesity, and low exercise capacity also contribute to this exaggerated response. Our recent preliminary data suggest that the cognitive benefits of exercise may be diminished in individuals who exhibit an exaggerated BP response during exercise. This implies that individuals with an exaggerated BP response, despite having normal resting BP, may not fully benefit from exercise. In this perspective paper, we review the physiological aspects of this phenomenon and explore strategies to address it. Additionally, we discuss BP responses in athletes within this content. Our goal is to prevent disease while maximizing the benefits of exercise for healthy individuals with an exaggerated BP response, as well as for elderly and cardiovascular patients.
The Effect of Lacticaseibacillus paracasei LPC100 and Lactiplantibacillus plantarum LP140 on Bone Mineral Density in Postmenopausal Women: A Multicenter, Randomized, Placebo-Controlled Study
Authors: Głogowska-Szeląg Joanna , Palka-Kisielowska Ilona , Porawska Wiesława , Bierła Joanna B. , Szczepankowska Agnieszka K. , Aleksandrzak-Piekarczyk Tamara , Cukrowska Bożena
Published: October 2024

Objectives: modulation of gut microbiota by probiotics has been proposed as a target for intervention to reduce bone mineral density (BMD) loss in the postmenopausal period. This study aims to evaluate the effect of Lacticaseibacillus (L.) paracasei LPC100 and Lactiplantibacillus (L.) plantarum LP140 on BMD in postmenopausal women in a multicenter, randomized, double-blind, placebo-controlled trial. Methods: the primary outcome was the change in T-score of the lumbar spine measured by dual-energy X-ray absorptiometry assessed after twelve-month probiotic supplementation. Secondary outcomes included changes in serological markers of inflammation and calcium–phosphate metabolism, body mass index, gastrointestinal symptoms, and satisfaction with the intervention. Results: a decrease in T-score indicating the progressive bone demineralization reached a statistically significant difference in the placebo group (from mean values of 0.06 ± 1.04 to −0.28 ± 1.12, p = 0.041) but not in the probiotic group (0.19 ± 0.99 and −0.08 ± 1.05, respectively, p = 0.125) with a p-value = 0.089 between the groups. No significant differences were found in secondary outcomes with the exception of vitamin D concentration and a significant reduction in some gastrointestinal symptoms in the probiotic group. A significant decrease in vitamin D level was observed only in the placebo group (p = 0.014). Probiotics were safe and well tolerated. Conclusions: this study suggests that the oral administration of L. paracasei LPC100 and L. plantarum LP140 may be a viable strategy to prevent BMD loss and vitamin D deficiency in postmenopausal women, but further research is necessary to confirm clinical benefits and to know the mechanism of action [ClinicalTrial.gov NCT06375668].
Detection of Thrombosis Using Soluble C-Type Lectin-like Receptor-2 with D-Dimer Level and Platelet Count
Authors: Wada Hideo , Shiraki Katsuya , Yamamoto Akitaka , Kamon Toshitaka , Masuda Jun , Ichikawa Yuhuko , Kawamura Masahide , Shimaoka Motomu , Simpo Hideto
Published: October 2024

Introduction: Soluble C-type lectin-like receptor -2 (sCLEC-2) has been recognized as a marker of platelet activation, and attention has been drawn to formulas combining sCLEC-2 levels with platelet count and D-dimer levels. Methods: In this study, sCLEC-2 levels, as well as sCLEC-2/platelet count (sCLEC-2/PLT), sCLEC-2 × D-dimer (sCLEC-2xDD), and sCLEc-2xDD/PLT formulas were used to detect thrombotic diseases, including microvascular thrombosis (MVT), arterial thromboembolism (ATE), and venous thromboembolism (VTE), with the aim of evaluating the ability of the three parameters combined in these formulas to diagnose thrombotic diseases. Results: The plasma sCLEC-2 levels were significantly higher in patients with infectious or thrombotic diseases than in those with neither thrombosis nor infection; however, there was no significant difference among patients with infection, ATE, VTE, and MVT; the correlations among sCLEC-2, platelet count, and D-dimer level were poor. The sCLEC-2/PLT ratio was the highest in patients with MVT, and the sCLEC-2 × D-dimer value was higher in patients with MVT and VTE than in those with neither thrombosis nor infection. Although receiver operating characteristic (ROC) analysis shows the differential diagnosis of thrombotic diseases from non-thrombosis without infection, the sCLEC-2 × D-dimer/platelet count was useful for differential diagnosis among MVT and infection or non-thrombotic diseases. Conclusions: sCLEC-2 is useful for the diagnosis of thrombosis, and the formulas of sCLEC-2 with platelet count or D-dimer are useful for the diagnosis of thrombosis using ROC analyses for the thrombosis group vs. the non-thrombosis group without infection.
Impact of Inhaled Nitric Oxide (iNO) on the Outcome of COVID-19 Associated ARDS
Authors: Stoll Sandra Emily , Böttiger Bernd W. , Dusse Fabian , Leister Nicolas , Leupold Tobias , Menzel Christoph , Overbeek Remco , Mathes Alexander
Published: October 2024

Background: Inhaled nitric oxide (iNO) can improve oxygenation in acute respiratory syndrome (ARDS), has anti-inflammatory and antithrombotic effects, and can inhibit coronavirus- replication. The study aim was to investigate the impact of iNO in COVID-19 associated ARDS (CARDS) on oxygenation, the length of mechanical ventilation (MV), the level of inflammatory markers and the rate of thrombotic events during ICU stay. Methods: This was a retrospective, observational, monocentric study analyzing the effect of INO (15 parts per million) vs. non-iNO in adult ventilated CARDS patients on oxygenation, the level of inflammatory markers, and the rate of thrombotic events during ICU stay. Within the iNO group, the impact on gas exchange was assessed by comparing arterial blood gas results obtained at different time points. Results: Overall, 19/56 patients were treated with iNO, with no difference regarding sex, age, body mass index, and SOFA-/APACHE II- score between the iNO and non-iNO groups. iNO improved oxygenation in iNO-responders (7/19) and had no impact on inflammatory markers or the rate of thrombotic events but was associated with an increased MV length. Conclusions: iNO was able to improve oxygenation in CARDS in iNO-responders but did not show an impact on inflammatory markers or the rate of thrombotic events, while it was associated with an increased MV length.
Elevated Circulating Levels of Gut Microbe-Derived Trimethylamine N-Oxide Are Associated with Systemic Sclerosis
Authors: Ho Karen J. , Muhammad Lutfiyya N. , Khanh Linh Ngo , Li Xinmin S. , Carns Mary , Aren Kathleen , Kim Seok-Jo , Verma Priyanka , Hazen Stanley L. , Varga John
Published: October 2024

Background/Objectives: Alterations in fecal microbial communities in patients with systemic sclerosis (SSc) are common, but the clinical significance of this observation is poorly understood. Gut microbial production of trimethylamine (TMA), and its conversion by the host to trimethylamine N-oxide (TMAO), has clinical and mechanistic links to cardiovascular and renal diseases. Direct provision of TMAO has been shown to promote fibrosis and vascular injury, hallmarks of SSc. We sought to determine levels of TMAO and related metabolites in SSc patients and investigate associations between the metabolite levels with disease features. Methods: This is an observational case:control study. Adults with SSc (n = 200) and non-SSc controls (n = 400) were matched for age, sex, indices of renal function, diabetes mellitus, and cardiovascular disease. Serum TMAO, choline, betaine, carnitine, γ-butyrobetaine, and crotonobetaine were measured using stable isotope dilution liquid chromatography tandem mass spectrometry. Results: Median TMAO concentration was higher (p = 0.020) in SSc patients (3.31 [interquartile range 2.18, 5.23] µM) relative to controls (2.85 [IQR 1.88, 4.54] µM). TMAO was highest among obese and male SSc participants compared to all other groups. Following adjustment for sex, BMI, age, race, and eGFR in a quantile regression model, elevated TMAO levels remained associated with SSc at each quantile of TMAO. Conclusions: Patients with SSc have increased circulating levels of TMAO independent of comorbidities including age, sex, renal function, diabetes mellitus, and cardiovascular disease. As a potentially modifiable factor, further studies examining the link between TMAO and SSc disease severity and course are warranted.
Depressive and Anxiety Symptoms in Community-Dwelling Women in Rural Areas of Greece in the Post-COVID-19 Pandemic Era
Authors: Peritogiannis Vaios , Mantziou Alexandra , Vaitsis Nikolaos , Aggelakou-Vaitsi Stamatina , Bakola Maria , Jelastopulu Eleni
Published: October 2024

Background/Objectives: Depressive and anxiety syndromes are associated with elevated disability and are more prevalent in women. Data on the prevalence of depressive and anxiety disorders in the rural context are limited and contradictory. It is relevant to study common mental disorders in rural areas in the most vulnerable population of women, particularly in the post-COVID-19 pandemic era. Methods: This is a cross-sectional study that was conducted in two primary healthcare sites in the rural region of Farsala, Central Greece after the obviation of all restrictive measures that had been posed due to the COVID-19 pandemic. All consecutive female patients that attended the study sites for any non-emergent reason were asked to participate in the study. For the recording of symptoms of depression and anxiety, the self-reported Depression, Anxiety, Stress Scale-21 (DASS-21) was used. Results: The study sample consisted of 129 women. The majority of participants were >50 years, with 27.9% being older adults. A small percentage (13.2%) suffered a chronic physical disease. A large proportion of the sample, slightly exceeding 40%, reported clinically relevant symptoms of anxiety, whereas a lower percentage of women with depressive symptoms was detected (17.1%). Symptoms of anxiety and depression were found to be interrelated, while a number of sociodemographic variables were associated with both, such as older age, education (primary), living status (alone, OR 123.5; 95% CI: 7.3–2098.8 for anxiety; OR 3.5; 95% CI: 1.3–9.8 for depression), employment (not working, (OR 0.157; 95% CI: 0.06–0.41 for anxiety; OR 0.08; 95% CI: 0.01–0.62 for depression) and the history of a chronic disease (OR 33.8; 95% CI: 4.3–264.7 for anxiety; OR 37.2; 95% CI: 10–138.1 for depression). Self-rated financial status was not related to symptoms of anxiety or depression. Conclusions: The study highlights the importance of inquiring for symptoms of depression and anxiety in women attending the rural primary care setting. The use of valid and reliable self-reported instruments that are easy to administrate may be helpful in this regard.
Early Fever in Allogeneic Stem Cell Transplantation: Are Presepsin and YKL-40 Valuable Diagnostic Tools?
Authors: Babel Jakša , Košuta Iva , Vujaklija Brajković Ana , Lončar Vrančić Ana , Premužić Vedran , Rogić Dunja , Duraković Nadira
Published: October 2024

Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a lifesaving treatment but carries a high infection risk. Diagnosing infections remains challenging due to the limited accuracy of standard biomarkers. Methods: This single-center study aimed to evaluate presepsin (PSP) and YKL-40 as infection biomarkers in febrile patients during the allo-HSCT pre-engraftment phase. Biomarker levels were prospectively measured in 61 febrile episodes from 54 allo-HSCT patients at admission, representing baseline levels, and then at Day 1, 3, 5, and 7 following fever onset. The diagnostic value was compared to that of procalcitonin (PCT). Results: PSP showed fair diagnostic value on Day 1 (AUC 0.656; 95% CI: 0.510–0.802) and Day 3 (AUC 0.698; 95% CI: 0.559–0.837). YKL-40 did not provide any significant diagnostic value across measured time points. PCT outperformed PSP and YKL-40, particularly on Day 3 (AUC 0.712; 95% CI: 0.572–0.852). When combining biomarkers, the best model for predicting infection used PSP > 3.144 ng/mL and PCT > 0.28 μg/L on Day 3, resulting in R2 of about 31% (p < 0.001). Conclusions: Neither test showed sufficient discriminative power for early infection to recommend their use as individual diagnostic tools in clinical practice.
Polysaccharide-Based Bioplastics: Eco-Friendly and Sustainable Solutions for Packaging
Authors: Gamage Ashoka , Thiviya Punniamoorthy , Liyanapathiranage Anuradhi , Wasana M. L. Dilini , Jayakodi Yasasvi , Bandara Amith , Manamperi Asanga , Dassanayake Rohan S. , Evon Philippe , Merah Othmane , Madhujith Terrence
Published: October 2024

Over the past few decades, synthetic petroleum-based packaging materials have increased, and the production of plastics has surpassed all other man-made materials due to their versatility. However, the excessive usage of synthetic packaging materials has led to severe environmental and health-related issues due to their nonbiodegradability and their accumulation in the environment. Therefore, bio-based packages are considered alternatives to substitute synthetic petroleum-based packaging material. Furthermore, the choice of packing material in the food industry is a perplexing process as it depends on various factors, such as the type of food product, its sustainability, and environmental conditions. Interestingly, due to proven mechanical, gas, and water vapor barrier properties and biological activity, polysaccharide-based bioplastics show the potential to expand the trends in food packaging, including edible films or coatings and intelligent and active food packaging. Various chemical modifications, network designs, and processing techniques have transformed polysaccharide materials into valuable final products, particularly for large-scale or high-value applications. Transitioning from petroleum-based resources to abundant bio-based polysaccharides presents an opportunity to create a sustainable circular economy. The economic viability of polysaccharide-based bioplastics is determined by several factors, including raw material costs, production technologies, market demand, and scalability. Despite their potential advantages over traditional plastics, their economic feasibility is affected by continuous technological advancements and evolving market dynamics and regulations. This review discusses the structure, properties, and recent developments in polysaccharide-based bioplastics as green and sustainable food packaging materials.
Influence of Physical Exercise on the Rehabilitation of Volumetric Muscle Loss Injury Reconstructed with Autologous Adipose Tissue
Authors: Lopez-Espejo Maria E. , Jimena Ignacio , Gil-Belmonte Maria-Jesus , Rivero Jose-Luis L. , Peña-Amaro Jose
Published: October 2024

Background: In volumetric muscle loss (VML) injuries, spontaneous muscle regeneration capacity is limited. The implantation of autologous adipose tissue in the affected area is an option to treat these lesions; however, the effectiveness of this therapy alone is insufficient for a complete recovery of the damaged muscle. This study examined the influence of treadmill exercise on the rehabilitation of VML injuries reconstructed with autologous adipose tissue, as a strategy to counteract the limitations of spontaneous regeneration observed in these injuries. Methods: Forty adult male Wistar rats were divided into eight groups of five individuals each: normal control (NC), regenerative control (RC), VML control (VML), VML injury reconstructed with fresh autologous adipose tissue (FAT), exercise-rehabilitated control (RNC), exercise-rehabilitated regenerative control (RRC), exercise-rehabilitated VML injury (RVML), and exercise-rehabilitated VML injury reconstructed with fresh autologous adipose tissue (RFAT). Histological and histochemical staining techniques were used for the analysis of structural features and histomorphometric parameters of the tibialis anterior muscle. Grip strength tests were conducted to assess muscle force. Results: Exercise rehabilitation decreased the proportion of disoriented fibers in RFAT vs. FAT group. The percentage of fibrosis was significantly higher in FAT and RFAT groups versus NC and RNC groups but did not vary significantly between FAT and RFAT groups. Overall, muscle grip strength and fiber size increased significantly in the exercise-rehabilitated groups compared to control groups. Conclusions: To conclude, rehabilitation with physical exercise tended to normalize the process of muscle repair in a model of VML injury reconstructed with fresh autologous adipose tissue, but it did not reduce the intense fibrosis associated with these injuries.
Segmentation of Glacier Area Using U-Net through Landsat Satellite Imagery for Quantification of Glacier Recession and Its Impact on Marine Systems
Authors: Robbins Edmund , Breininger Robert D. , Jiang Maxwell , Madera Michelle , White Ryan T. , Kachouie Nezamoddin N.
Published: October 2024

Glaciers have experienced a global trend of recession within the past century. Quantification of glacier variations using satellite imagery has been of great interest due to the importance of glaciers as freshwater resources and as indicators of climate change. Spatiotemporal glacier dynamics must be monitored to quantify glacier variations. The potential methods to quantify spatiotemporal glacier dynamics with increasing complexity levels include detecting the terminus location, measuring the length of the glacier from the accumulation zone to the terminus, quantifying the glacier surface area, and measuring glacier volume. Although some deep learning methods designed purposefully for glacier boundary segmentation have achieved acceptable results, these models are often localized to the region where their training data were acquired and further rely on the training sets that were often curated manually to highlight glacial regions. Due to the very large number of glaciers, it is practically impossible to perform a worldwide study of glacier dynamics using manual methods. As a result, an automated or semi-automated method is highly desirable. The current study has built upon our previous works moving towards identification methods of the 2D glacier profile for glacier area segmentation. In this study, a deep learning method is proposed for segmentation of temporal Landsat images to quantify the glacial region within the Mount Cook/Aoraki massif located in the Southern Alps/Kā Tiritiri o te Moana of New Zealand/Aotearoa. Segmented glacial regions can be further utilized to determine the relationship of their variations due to climate change. This model has demonstrated promising performance while trained on a relatively small dataset. The permanent ice and snow class was accurately segmented at a 92% rate by the proposed model.
In Vitro and In Vivo Genotoxicity of Polystyrene Microplastics: Evaluation of a Possible Synergistic Action with Bisphenol A
Authors: Santovito Alfredo , Lambertini Mattia , Nota Alessandro
Published: October 2024

The ubiquitous presence of plastics represents a global threat for all ecosystems and human health. In this study, we evaluated, in vitro and in vivo, the genotoxic potential of different concentrations of polystyrene microplastics (PS-MPs) and their possible synergistic interactions with bisphenol-A (BPA). For the in vitro and the in vivo assays, we used human lymphocytes and hemocytes from Lymnaea stagnalis, respectively. The genomic damage was evaluated by the micronucleus assay, and differences in eggs laid and growth of L. stagnalis were also evaluated. In human lymphocytes, PS-MPs alone at the concentration of 200 μg/mL and in association with BPA 0.100 µg/mL significantly increased the frequencies of micronuclei and nuclear buds, indicating a possible in vitro genotoxic additive action of these two compounds. Vice versa, PS-MPs did not result in genotoxicity in hemocytes. Our results indicated that PS-MPs have genotoxic properties only in vitro and at a concentration of 200 µg/mL; moreover, this compound could intensify the genomic damage when tested with BPA, indicating possible cumulative effects. Finally, PS significantly reduced the growth and the number of laid eggs in L. stagnalis.
Organic Carbon Storage in Waterlogging Soils in Ávila, Spain: A Traditional Agrosilvopastoral Region
Authors: Alvarez-Castellanos María P. , Escudero-Campos Laura , Mongil-Manso Jorge , San Jose Francisco J. , Jiménez-Sánchez Adrián , Jiménez-Ballesta Raimundo
Published: October 2024

Soils play a crucial role in the protection, management, and ecological understanding of the La Moraña region, located in Ávila province, Central Spain, which has a moderate population, traditional agriculture, livestock farming, and low industrial activity, resulting in relatively low environmental degradation. The region’s soils often experience prolonged water stagnation, influencing its agronomy, ecology, and economy. This study aimed to estimate and understand the soil’s role in the C sequestration of an agrosilvopastoral system under conditions of temporary water stagnation and different land uses. The results showed that ryegrass-magaza and Pinus pinaster show more content in soil carbon sequestration storage (98.7 and 92.4 Mg per hectare) compared to the adjacent degraded rangeland (75.8 and 63.9 Mg ha−1). Arenosols exhibited a higher total amount of SOC stocks. The soil profile with ryegrass sequestered more nitrogen (9.7 Mg ha−1) than other land uses; moreover, Arenosols have a lower nitrogen sequestration capacity even in low-forest conditions. The study highlights significant differences in carbon accumulation due to the management practices, temporary water layers, and parent material.
Laboratory Model Tests on the Deformation and Failure of Terraced Loess Slopes Induced by Extreme Rainfall
Authors: Jia Jun , Pei Xiangjun , Guo Xiaopeng , Cui Shenghua , Sun Pingping , Fan Haoran , Zhang Xiaochao , Gu Qi
Published: October 2024

Heavy rainfall is the main factor inducing the failure of loess slopes. However, the failure mechanism and mode of terraced loess slopes under heavy rainfall have not been well investigated and understood. This paper presents the experimental study on the deformation and failure of terraced loess slopes with different gradients under extreme rainfall conditions. The deformation and failure processes of the slope and the migration of the wetting front within the slope during rainfall were captured by the digital cameras installed on the top and side of the test box. In addition, the mechanical and hydrological responses of the slope, including earth pressure, water content, pore water pressure, and matric suction, were monitored and analyzed under rainfall infiltration and erosion. The experimental study shows that the deformation and failure of terraced loess slopes under heavy rainfall conditions exhibit the characteristic of progressive erosion damage. In general, the steeper the slope, the more severe the deformation and failure, and the shorter the time required for erosion failure. The data obtained from sensors embedded in the slope can reflect the mechanical and hydraulic characteristics of the slope in response to rainfall. The earth pressure and pore water pressure in the slope exhibit a fluctuating pattern with continued rainfall. The failure mode of terraced loess slopes under extreme rainfall can be summarized into five stages: erosion of slope surface and formation of small gullies and cracks, expansion of gullies and cracks along the slope surface, widening and deepening of gullies, local collapse and flow-slip of the slope, and large-scale collapse of the slope. The findings can provide preliminary data references for researchers to better understand the failure characteristics of terraced loess slopes under extreme rainfall and to further validate the results of numerical simulations and analytical solutions.
Global Gain, but Local Loss—National Park and Municipal Revenues in Poland
Authors: Kachniarz Marian
Published: October 2024

The paper analyzes the problem of stagnation in the creation of new national parks in Poland, identifying key barriers and proposing solutions. The background to the study refers to the role of national parks in nature conservation, but at the same time highlights the problem of the limited development of the park network in Poland, which remains at 1.05% of the country’s area—well below the EU average. The main obstacles identified by the author are the resistance of local governments due to the reduction in forest tax revenues and the differences in salaries between employees of national parks and state forests. Research methods included legal and economic analysis and a review of the literature on national park management. The results indicate that the problem can be solved by adequate financial compensation to local communities and the introduction of a co-management model that integrates the interests of local governments and parks. The article suggests that a change in the approach of the government administration and a more participatory management model can help to break the stagnation and increase the number of national parks, which is relevant not only for Poland but also for other countries struggling with similar challenges.