bluuebunny/crossref_metadata_2025 huggingface dataset:
The Autobot-WQ: A portable, low-cost autosampler to provide new insight into urban spatio-temporal water quality dynamics
Authors: Khamis Kieran , Ouellet Valerie , Croghan Danny , Hernandez Gonzalez Liliana M. , Packman Aaron I. , Hannah David M. , Krause Stefan
Published: April 2023

Urbanization and the increase in urban land cover are growing concerns associated with numerous negative impacts on surface water quality. Currently, many emerging contaminants are difficult to measure with no field deployable sensors currently available. Hence, discrete grab samples are required for subsequent laboratory analysis. To capture the spatiotemporal variability in pollution pulses, autosamplers can be used, but commercial offerings are both expensive and have a large footprint. This can be problematic in urban environments where there is a high density of point source inputs and risk of vandalism or theft. Here, we present a small and robust low-cost autosampler that is ideally suited for deployment in urban environments. The design is based on “off the shelf” open-source hardware components and software and requires no prior engineering, electronics, or computer programming experience to build. The autosampler uses a small peristaltic pump to enable collection of 14 small volume samples (50 mL) and is housed in a small footprint camera case. To illustrate the technology, we present two use cases for rapid sampling of stormwater pulses of: 1) an urban river channel and 2) green roof runoff. When compared with a commercial autosampler, our device showed comparable results and enabled us to capture temporal dynamics in key water quality parameters (e.g., dissolved organic matter) following rain events in an urban stream. Water quality differences associated with differing green roof design/maintenance regimes (managed and unmanaged vegetation) were captured using the autosampler, highlighting how unmanaged vegetation has a greater potential for mitigating the rapid runoff and peaked pollutant inputs associated with impervious surfaces. These two case studies show that our portable autosampler provides capacity to improve understanding of the impact of urban design and infrastructure on water quality and can lead to the development of more effective mitigation solutions. Finally, we discuss opportunities for further technical refinement of our autosampler and applications to improve environmental monitoring. We propose a holistic monitoring approach to address some of the outstanding challenges in urban areas and enable monitoring to shift from discrete point sources towards characterization of catchment or network scale dynamics.
Transcriptomic analysis identified SLC40A1 as a key iron metabolism-related gene in airway macrophages in childhood allergic asthma
Authors: Wang Zhili , He Yu , Cun Yupeng , Li Qinyuan , Zhao Yan , Luo Zhengxiu
Published: April 2023

Introduction: Asthma is the most common chronic condition in children, with allergic asthma being the most common phenotype, accounting for approximately 80% of cases. Growing evidence suggests that disruption of iron homeostasis and iron regulatory molecules may be associated with childhood allergic asthma. However, the underlying molecular mechanism remains unclear.

Methods: Three childhood asthma gene expression datasets were analyzed to detect aberrant expression profiles of iron metabolism-related genes in the airways of children with allergic asthma. Common iron metabolism-related differentially expressed genes (DEGs) across the three datasets were identified and were subjected to functional enrichment analysis. Possible correlations between key iron metabolism-related DEGs and type 2 airway inflammatory genes were investigated. Single-cell transcriptome analysis further identified major airway cell subpopulations driving key gene expression. Key iron metabolism-related gene SLC40A1 was validated in bronchoalveolar lavage (BAL) cells from childhood asthmatics with control individuals by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunofluorescence. The intracellular iron content in BAL cells was assessed by Perls iron staining and the iron levels in BAL supernatant was measured by iron assay to assess airway iron metabolism status in childhood asthmatics.

Results: Five common iron metabolism-related DEGs were identified, which were functionally related to iron homeostasis. Among these genes, downregulated SLC40A1 was strongly correlated with type 2 airway inflammatory markers and the gene signature of SLC40A1 could potentially be used to determine type 2-high and type 2-low subsets in childhood allergic asthmatics. Further single-cell transcriptomic analysis identified airway macrophages driving SLC40A1 expression. Immunofluorescence staining revealed colocalization of FPN (encoded by SLC40A1) and macrophage marker CD68. Down-regulation of SLC40A1 (FPN) was validated by qRT-PCR and immunofluorescence analysis. Results further indicated reduced iron levels in the BAL fluid, but increased iron accumulation in BAL cells in childhood allergic asthma patients. Furthermore, decreased expression of SLC40A1 was closely correlated with reduced iron levels in the airways of children with allergic asthma.

Discussion: Overall, these findings reveal the potential role of the iron metabolism-related gene SLC40A1 in the pathogenesis of childhood allergic asthma.
Alterations in cytoskeletal and Ca2+ cycling regulators in atria lacking the obscurin Ig58/59 module
Authors: Grogan Alyssa , Huang Weiliang , Brong Annie , Kane Maureen A. , Kontrogianni-Konstantopoulos Aikaterini
Published: April 2023

Introduction

Obscurin (720–870 kDa) is a giant cytoskeletal and signaling protein that possesses both structural and regulatory functions in striated muscles. Immunoglobulin domains 58/59 (Ig58/59) of obscurin bind to a diverse set of proteins that are essential for the proper structure and function of the heart, including giant titin, novex-3, and phospholamban (PLN). Importantly, the pathophysiological significance of the Ig58/59 module has been further underscored by the discovery of several mutations within Ig58/59 that are linked to various forms of myopathy in humans. We previously generated a constitutive deletion mouse model, Obscn-ΔIg58/59, that expresses obscurin lacking Ig58/59, and characterized the effects of this deletion on cardiac morphology and function through aging. Our findings demonstrated that Obscn-ΔIg58/59 male animals develop severe arrhythmia, primarily manifesting as episodes of junctional escape and spontaneous loss of regular p-waves, reminiscent of human atrial fibrillation, accompanied by significant atrial enlargement that progresses in severity with aging.

Methods and Results

To comprehensively characterize the molecular alterations responsible for these pathologies, we performed proteomic and phospho-proteomic analyses in aging Obscn-ΔIg58/59 atria. Our studies revealed extensive and novel alterations in the expression and phosphorylation profile of major cytoskeletal proteins, Ca2+ regulators, and Z-disk associated protein complexes in the Obscn-ΔIg58/59 atria through aging.

Discussion

These studies implicate obscurin, particularly the Ig58/59 module, as an essential regulator of the Z-disk associated cytoskeleton and Ca2+ cycling in the atria and provide new molecular insights into the development of atrial fibrillation and remodeling.
Artificial intelligence-estimated biological heart age using a 12-lead electrocardiogram predicts mortality and cardiovascular outcomes
Authors: Baek Yong-Soo , Lee Dong-Ho , Jo Yoonsu , Lee Sang-Chul , Choi Wonik , Kim Dae-Hyeok
Published: April 2023

Background

There is a paucity of data on artificial intelligence-estimated biological electrocardiography (ECG) heart age (AI ECG-heart age) for predicting cardiovascular outcomes, distinct from the chronological age (CA). We developed a deep learning-based algorithm to estimate the AI ECG-heart age using standard 12-lead ECGs and evaluated whether it predicted mortality and cardiovascular outcomes.

Methods

We trained and validated a deep neural network using the raw ECG digital data from 425,051 12-lead ECGs acquired between January 2006 and December 2021. The network performed a holdout test using a separate set of 97,058 ECGs. The deep neural network was trained to estimate the AI ECG-heart age [mean absolute error, 5.8 ± 3.9 years; R-squared, 0.7 (r = 0.84, p < 0.05)].

Findings

In the Cox proportional hazards models, after adjusting for relevant comorbidity factors, the patients with an AI ECG-heart age of 6 years older than the CA had higher all-cause mortality (hazard ratio (HR) 1.60 [1.42–1.79]) and more major adverse cardiovascular events (MACEs) [HR: 1.91 (1.66–2.21)], whereas those under 6 years had an inverse relationship (HR: 0.82 [0.75–0.91] for all-cause mortality; HR: 0.78 [0.68–0.89] for MACEs). Additionally, the analysis of ECG features showed notable alterations in the PR interval, QRS duration, QT interval and corrected QT Interval (QTc) as the AI ECG-heart age increased.

Conclusion

Biological heart age estimated by AI had a significant impact on mortality and MACEs, suggesting that the AI ECG-heart age facilitates primary prevention and health care for cardiovascular outcomes.
Consumer feces impact coral health in guild-specific ways
Authors: Grupstra Carsten G. B. , Howe-Kerr Lauren I. , van der Meulen Jesse A. , Veglia Alex J. , Coy Samantha R. , Correa Adrienne M. S.
Published: April 2023

Animal waste products are an important component of nutrient cycles and result in the trophic transmission of diverse microorganisms. There is growing recognition that the feces of consumers, such as predators, may impact resource species, their prey, via physical effects and/or microbial activity. We tested the effect of feces from distinct fish trophic groups on coral health and used heat-killed fecal controls to tease apart physical versus microbial effects of contact with fecal material. Fresh grazer/detritivore fish feces caused lesions more frequently on corals, and lesions were 4.2-fold larger than those from sterilized grazer/detritivore feces; in contrast, fresh corallivore feces did not cause more frequent or larger lesions than sterilized corallivore feces. Thus, microbial activity in grazer/detritivore feces, but not corallivore feces, was harmful to corals. Characterization of bacterial diversity in feces of 10 reef fish species, ranging from obligate corallivores to grazer/detritivores, indicated that our experimental findings may be broadly generalizable to consumer guild, since feces of some obligate corallivores contained ~2-fold higher relative abundances of coral mutualist bacteria (e.g., Endozoicomonadaceae), and lower abundances of the coral pathogen, Vibrio coralliilyticus, than feces of some grazer/detritivores. These findings recontextualize the ecological roles of consumers on coral reefs: although grazer/detritivores support coral reef health in various ways (e.g., promoting coral settlement and herbivory through the removal of detritus and sediments from the algal matrix), they also disperse coral pathogens. Corallivore predation can wound corals, yet their feces contain potentially beneficial coral-associated bacteria, supporting the hypothesized role of consumers, and corallivores in particular, in coral symbiont dispersal. Such consumer-mediated microbial dispersal as demonstrated here has broad implications for environmental management.
Consolidation characteristics of high-water-content slurries improved by flocculation-enhanced surcharge (vacuum) preloading method
Authors: Han Chao , Fan Zhou , Zhang Rongjun , Cheng Jinkang , Sun Ke , Peng Qian
Published: April 2023

The high volume and unfavorable properties of high-water-content slurries make their treatment difficult and costly. Improving the slurries through prefabricated drains combined with surcharge or vacuum preloading represents an environmental and economic means of reusing these slurries as subsoil. As conventional methods are inefficient in dealing with low-permeability slurries, flocculation is often applied. In this study, the influence of the flocculant dosage on the drainage behavior is observed, and the secondary consolidation of the slurries is examined through laboratory tests. Flocculation is found to accelerate the consolidation process, although the flocculant dosage has an optimal value when combined with the surcharge preloading method. The coefficient of secondary consolidation is more significant in slurries improved by the flocculation-enhanced surcharge (vacuum) preloading method than in the case without flocculation. The use of flocculation must be carefully controlled.
Tobacco Root Microbial Community Composition Significantly Associated With Root-Knot Nematode Infections: Dynamic Changes in Microbiota and Growth Stage
Authors: Cao Yi , Yang Zhi-Xiao , Yang Dong-Mei , Lu Ning , Yu Shi-Zhou , Meng Jian-Yu , Chen Xing-Jiang
Published: April 2023

The root-knot nematode (RKN) is an important pathogen that affects the growth of many crops. Exploring the interaction of biocontrol bacteria-pathogens-host root microbes is the theoretical basis for improving colonization and controlling the effect of biocontrol bacteria in the rhizosphere. Therefore, 16S and 18S rRNA sequencing technology was used to explore the microbial composition and diversity of tobacco roots (rhizosphere and endophytic) at different growth stages in typical tobacco RKN-infected areas for 2 consecutive years. We observed that RKN infection changed the α-diversity and microbial composition of root microorganisms and drove the transformation of microorganisms from bacteria to fungi. The abundance of Sphingomonas decreased significantly from 18% to less than 3%, while the abundance of Rhizobiaceae increased from 4 to 15% at the early growth stage during the first planting year, and it promoted the proliferation of Chryseobacterium at the late growth stage in rhizosphere microorganisms with the highest abundance of 17%. The overall trend of rhizosphere microorganisms changed in the early growth stage with increasing growth time. The specific results were as follows: (1) Rhizobiaceae and Chryseobacterium increased rapidly after 75 days, became the main abundant bacteria in the rhizosphere microorganisms. (2) The dominant flora in fungi were Fusarium and Setophoma. (3) Comparing the root microbes in 2017 and 2018, RKN infection significantly promoted the proliferation of Pseudomonas and Setophoma in both the rhizosphere and endophytes during the second year of continuous tobacco planting, increasing the relative abundance of Pseudomonas from 2 to 25%. Pseudomonas was determined to play an important role in plant pest control. Finally, a total of 32 strains of growth-promoting bacteria were screened from tobacco rhizosphere bacteria infected with RKN through a combination of 16S rRNA sequencing and life-promoting tests. The results of this research are helpful for analyzing the relationship between RKNs and bacteria in plants, providing reference data for elucidating the pathogenesis of RKNs and new ideas for the biological control of RKNs.
Escherichia coli from biopsies differ in virulence genes between patients with colorectal neoplasia and healthy controls
Authors: Bosák Juraj , Kohoutová Darina , Hrala Matěj , Křenová Jitka , Morávková Paula , Rejchrt Stanislav , Bureš Jan , Šmajs David
Published: April 2023

Introduction

Pathogenic strains of Escherichia coli have been clearly identified as the causative agents of extraintestinal and diarrheal infections; however, the etiopathogenic role of E. coli in other conditions, including colorectal cancer, remains unclear.

Methods

This study aimed to characterize mucosal E. coli isolates (n = 246) from 61 neoplasia patients and 20 healthy controls for the presence of 35 genetic determinants encoding known virulence factors.

Results

Virulence determinants encoding invasin (ibeA), siderophore receptor (iroN), S-fimbriae (sfa), and genotoxin (usp) were more prevalent among E. coli isolated from patients with neoplasia compared to the control group (p < 0.05). In addition, the prevalence of these virulence determinants was increased in more advanced neoplasia stages (padj < 0.0125). Compared to patients with advanced colorectal adenoma and carcinoma, the ibeA gene was rarely found in the control group and among patients with non-advanced adenoma (p < 0.05), indicating its potential as the advanced-neoplasia biomarker. Patients with neoplasia frequently had E. coli strains with at least one of the abovementioned virulence factors, whereby specific combinations of these virulence factors were found.

Discussion

These findings suggest that E. coli strains isolated from patients with colorectal neoplasia possess several virulence factors, which could contribute to the development of neoplastic processes in the large intestine.
Comprehensive analysis of metabolites produced by co-cultivation of Bifidobacterium breve MCC1274 with human iPS-derived intestinal epithelial cells
Authors: Sen Akira , Nishimura Tatsuki , Yoshimoto Shin , Yoshida Keisuke , Gotoh Aina , Katoh Toshihiko , Yoneda Yasuko , Hashimoto Toyoyuki , Xiao Jin-Zhong , Katayama Takane , Odamaki Toshitaka
Published: April 2023

Examining how host cells affect metabolic behaviors of probiotics is pivotal to better understand the mechanisms underlying the probiotic efficacy in vivo. However, studies to elucidate the interaction between probiotics and host cells, such as intestinal epithelial cells, remain limited. Therefore, in this study, we performed a comprehensive metabolome analysis of a co-culture containing Bifidobacterium breve MCC1274 and induced pluripotent stem cells (iPS)-derived small intestinal-like cells. In the co-culture, we observed a significant increase in several amino acid metabolites, including indole-3-lactic acid (ILA) and phenyllactic acid (PLA). In accordance with the metabolic shift, the expression of genes involved in ILA synthesis, such as transaminase and tryptophan synthesis-related genes, was also elevated in B. breve MCC1274 cells. ILA production was enhanced in the presence of purines, which were possibly produced by intestinal epithelial cells (IECs). These findings suggest a synergistic action of probiotics and IECs, which may represent a molecular basis of host-probiotic interaction in vivo.
Application of the pressure cooker technique for transarterial embolization of brain arteriovenous malformations: Factors affecting obliteration and outcomes
Authors: Lu Dan , Li Yuqian , Yang Zijian , Zhao Zhenwei , Fang Wei , Chen Lei , Ma Tao , Wang Naibing , Li Xueliang , Zhang Tao , Deng Jianping
Published: April 2023

Objective

The typical pressure cooker technique (PCT) and several modifications with similar mechanisms have been introduced to enhance the embolization of brain arteriovenous malformations (bAVMs). This study aimed to assess the effectiveness of transarterial embolization of bAVMs with the PCT.

Method

From January 2019 to December 2021, 125 consecutive patients with bAVM managed by transarterial embolization in the prospective database on cerebral vascular diseases of a single center were retrospectively reviewed. Patient data and lesion characteristics were collected. According to the treatment strategy, the patients were assigned to the PCT group (46 patients) and conventional embolization technique (CET) group (79 patients).

Results

Baseline patient features were comparable between the two groups. After the first procedure, complete obliteration immediately was observed in 61 and 42% of patients in the PCT and CET groups, respectively. The rate was markedly elevated in the PCT group (p = 0.04). In subgroup analysis, the rate of immediate complete obliteration was starkly increased in PCT group patients with Spetzler-Martin grade I/II bAVM (86 and 53% in the PCT and CET groups, respectively; p = 0.0036). The overall complication rates were similar in the two groups (13 and 10% in the PCT and CET groups, respectively; p = 0.77). In multivariable analysis, nidus size >3 cm (OR = 8.826, 95% CI: 1.250–62.312; p = 0.03) and deep location (OR = 8.576, 95% CI: 1.480–49.690; p = 0.02) were significant factors affecting complete obliteration in the PCT group.

Conclusion

The PCT may yield a higher rate of immediate complete obliteration with transarterial embolization of bAVMs, without increasing the rate of procedure-related complications.
Plasma lipid profiles and homocysteine levels in anti-N-methyl-D-aspartate receptor encephalitis
Authors: Wang Zhi-hao , Qiao Shan , Wang Lei , Wang Kemo , Zhang Ranran , Jin Yang , Wu Huai-kuan , Liu Xuewu
Published: April 2023

Introduction

We aimed to investigate whether lipid profiles and homocysteine levels in patients with anti-N-methyl-D-aspartate receptor encephalitis are related to clinical presentation and prognosis, which may contribute to further research on the pathogenesis and treatment of this disease.

Methods

This study included a total of 43 patients with anti-N-methyl-D-aspartate receptor encephalitis and 43 sex–age-matched healthy controls. Baseline demography, clinical data, patient outcomes, and ancillary examination results were recorded. Patients were followed up every 2–3 months during the first year. The modified Rankin Scale score was used to evaluate the therapeutic effect and clinical outcome.

Results

Among the 43 patients included in this study, 55.81% were male, the mean age of onset was 27 years old, and the median modified Rankin Scale score on admission was 3.0. Apolipoprotein A-1 was significantly lower in patients with anti-N-methyl-D-aspartate receptor encephalitis compared with healthy controls (p = 0.004). Compared with healthy controls, homocysteine (p = 0.002), apolipoprotein B (p = 0.004), Lpa (p = 0.045), and apolipoprotein B/apolipoprotein A-1 (p = 0.001) were significantly increased in patients with anti-N-methyl-D-aspartate receptor encephalitis. According to the modified Rankin Scale scores, 6 months after discharge, 72.09% of patients had a good prognosis and 27.91% had a poor prognosis. In the good prognosis group, age (p = 0.031), lipoprotein a (p = 0.023), apolipoprotein A-1 (p = 0.027) at baseline, and the modified Rankin Scale score on admission (p = 0.019) were significantly higher than those in the poor prognosis group.

Conclusion

This study suggests the possibility that serum lipid profile and homocysteine play an important role in the pathogenesis of anti-N-methyl-D-aspartate receptor encephalitis, providing support for lipid-lowering treatment of anti-N-methyl-D-aspartate receptor encephalitis patients.
Postnatal nutrition environment reprograms renal DNA methylation patterns in offspring of maternal protein-restricted stroke-prone spontaneously hypertensive rats
Authors: Ando Chika , Ma Sihui , Miyoshi Moe , Furukawa Kyohei , Li Xuguang , Jia Huijuan , Kato Hisanori
Published: April 2023

Maternal malnutrition hampers the offspring health by manipulating the epigenome. Recent studies indicate that the changes in DNA methylation could be reversed by afterbirth nutrition supplementation. In this study, we used DNA methylation arrays to comprehensively investigate the DNA methylation status of the renal promoter regions and the effects of postnatal protein intake on DNA methylation. We fed stroke-prone spontaneously hypertensive (SHRSP) rat dams a normal diet or a low-protein diet during pregnancy, and their 4-week-old male offspring were fed a normal diet or a high−/low-protein diet for 2 weeks. We found that the methylation status of 2,395 differentially methylated DNA regions was reprogrammed, and 34 genes were reset by different levels of postnatal protein intake in the offspring. Among these genes, Adora2b, Trpc5, Ar, Xrcc2, and Atp1b1 are involved in renal disease and blood pressure regulation. Our findings indicate that postnatal nutritional interventions can potentially reprogram epigenetic changes, providing novel therapeutic and preventive epigenetic targets for salt-sensitive hypertension.
Morphometric analysis of tumor microvessels for detection of hepatocellular carcinoma using contrast-free ultrasound imaging: A feasibility study
Authors: Sabeti Soroosh , Ternifi Redouane , Larson Nicholas B. , Olson Michael C. , Atwell Thomas D. , Fatemi Mostafa , Alizad Azra
Published: April 2023

Introduction

A contrast-free ultrasound microvasculature imaging technique was evaluated in this study to determine whether extracting morphological features of the vascular networks in hepatic lesions can be beneficial in differentiating benign and malignant tumors (hepatocellular carcinoma (HCC) in particular).

Methods

A total of 29 lesions from 22 patients were included in this work. A post-processing algorithm consisting of clutter filtering, denoising, and vessel enhancement steps was implemented on ultrasound data to visualize microvessel structures. These structures were then further characterized and quantified through additional image processing. A total of nine morphological metrics were examined to compare different groups of lesions. A two-sided Wilcoxon rank sum test was used for statistical analysis.

Results

In the malignant versus benign comparison, six of the metrics manifested statistical significance. Comparing only HCC cases with the benign, only three of the metrics were significantly different. No statistically significant distinction was observed between different malignancies (HCC versus cholangiocarcinoma and metastatic adenocarcinoma) for any of the metrics.

Discussion

Obtained results suggest that designing predictive models based on such morphological characteristics on a larger sample size may prove helpful in differentiating benign from malignant liver masses.
Pediatric Surgery and Anesthesia in Low-Middle Income Countries: Current Situation and Ethical Challenges
Authors: Pulvirenti Rebecca , Gortan Marianna , Cumba Dioniso , Gamba Piergiorgio , Tognon Costanza
Published: April 2023

Low-middle income countries (LMICs) are currently experiencing an important population growth, leading to a substantial raise in the number of children living in those areas. As a consequence, the existing gap between the need for surgical and anesthetic care and the available therapeutic options will increase. To overcome this, an improvement in the available expertise, infrastructures, and supplies will be mandatory. The implementation of educational and training programs for local healthcare providers should be a top priority. Alongside, the population's awareness on the necessity to seek for medical care should be deployed, together with an eased access to health facilities. Based on the existing literature and our 20-years' experience in humanitarian missions, our article aims to investigate the status of pediatric surgery in LMICs, and the role of western aids in the implementation of this ever-increasing field of expertise.
Comparison of pneumonitis risk between immunotherapy alone and in combination with chemotherapy: an observational, retrospective pharmacovigilance study
Authors: Li Huixia , Zheng Yifan , Xu Peihang , Li Zimu , Kuang Yukun , Feng Xiaoqing , He Junhao , Li Jia , Chen Xiao , Bai Lihong , Tang Ke-Jing
Published: April 2023

Importance: Checkpoint inhibitor pneumonitis (CIP) is a rare but serious adverse event that may impact treatment decisions. However, there is limited information comparing CIP risks between immune checkpoint inhibitor (ICI) monotherapy and combination with chemotherapy due to a lack of direct cross-comparison in clinical trials.

Objective: To determine whether ICI combination with chemotherapy is superior to ICI in other drug regimens (including monotherapy) in terms of CIP risk.

Study Design and Methods: This observational, cross-sectional and worldwide pharmacovigilance cohort study included patients who developed CIP from the World Health Organization database (WHO) VigiBase and the US Food and Drug Administration Adverse Event Reporting System (FAERS) database. Individual case safety reports (ICSR) were extracted from 2015 to 2020 in FAERS and from 1967 to 2020 in VigiBase. Timing and reporting odds ratio (ROR) of CIP in different treatment strategies were used to detect time-to-onset and the risk of pneumonitis after different immunotherapy regimens.

Results: A total of 93,623 and 114,704 ICI-associated ICSRs were included in this study from VigiBase and FAERS databases respectively. 3450 (3.69%) and 3278 (2.86%) CIPs occurred after therapy initiation with a median of 62 days (VigiBase) and 40 days (FAERS). Among all the CIPs, 274 (7.9%) and 537 (16.4%) CIPs were associated with combination therapies. ICIs plus chemotherapy combination was associated with pneumonitis in both VigiBase [ROR 1.35, 95% CI 1.18-1.52] and FAERS [ROR 1.39, 95% CI 1.27–1.53]. The combination of anti-PD-1 antibodies and anti-CTLA-4 antibodies with chemotherapy demonstrated an association with pneumonitis in both VigiBase [PD-1+chemotherapy: 1.76, 95% CI 1.52-2.05; CTLA-4+chemotherapy: 2.36, 95% CI 1.67-3.35] and FAERS [PD-1+chemotherapy: 1.70, 95% CI 1.52-1.91; CTLA-4+chemotherapy: 1.70, 95% CI 1.31-2.20]. Anti-PD-L1 antibodies plus chemotherapy combinations did not show the association.

Conclusion: Compared to ICI in other drug regimens (including monotherapy), the combination of ICI plus chemotherapy is significantly associated with higher pneumonitis toxicity. Anti-PD-1/CTLA4 medications in combination with chemotherapy should be obviated in patients with potential risk factors for CIP.

Trial Registration: clinicaltrials.gov, ChiCTR2200059067
The associations of decent work with wellbeing and career capabilities: a meta-analysis
Authors: Su Xuebing , Chan Ko Ling
Published: April 2023

Introduction

As a global sustainable development goal, the decent work notion has been promoted all over the world at theoretical, practical, and research levels for the purpose of enhancing people's capacity to enjoy freedom, equity, security, and human dignity at work. However, conclusive findings of the impact of decent work on people's wellbeing and longer-term career development are still missing due to a lack of systematic reviews on this topic. This study aims to (a) investigate the associations of decent work with people's wellbeing and their capabilities for sustaining career development and (b) examine the differential associations across different subgroups.

Methods

Databases of literature archived on or before 4 March 2022 were searched. A total of 46 studies met the inclusion criteria and were included in the analysis for the combined Pearson correlation coefficient (r) to estimate the associations of decent work with wellbeing and career capabilities, among which 30 studies (16,026 participants) were used for calculating the association between decent work and wellbeing whereas 26 studies (12,384 participants) were used for decent work and career capabilities.

Results and discussion

First, decent work demonstrates a medium association with wellbeing (r= .48, 95% CI [.45, .51]), and a medium association with career capabilities (r= .44, 95% CI [.40, .49]). Second, no significant differences with respect to the association of decent work with wellbeing and career capabilities were identified across subgroups categorized by developed/developing countries, population type, social status of participants as employee or student, participants from vulnerable/general groups, aspects of wellbeing/career capabilities, and study design. These results pose important implications for informing future research and practice to measure and promote decent work across the world.
Are happier adolescents more willing to protect the environment? Empirical evidence from Programme for International Student Assessment 2018
Authors: Zhang Min , Zhang Weidong , Shi Yong
Published: April 2023

A large number of existing studies have discussed the potential factors affecting pro-environmental behaviors (PEBs) in adolescents. However, few studies have focused on the possible impact of adolescents’ subjective wellbeing (SWB) on their PEBs. Why and how adolescents’ SWB affects their PEBs remains a puzzle. To unravel this puzzle, this paper aims to establish a suitable instrumental variable (IV) to correctly estimate the contribution of adolescents’ SWB to their PEBs. Using the international data from the Organisation for Economic Co-operation and Development (OECD) ‘s Programme for International Student Assessment 2018, we construct a unique dataset of eight countries or economies, which includes 56,374 samples related to the SWB and PEBs of 15-year-old students. In this paper, the days of physical education classes in school per week are used as the IV. Through a two-stage least squares method, we find that the contribution of adolescents’ SWB to PEBs is significantly positive. We also find that the pathway by which SWB improves PEBs works through adolescents’ self-efficacy. Furthermore, the results indicate that the positive impact of SWB on PEBs is more pronounced among adolescents with better peer relationships and stronger multicultural values. Our findings highlight the influence of positive affects in cultivating adolescent’ PEBs and the importance of growing up surrounded by happiness.
Changes in sleep quality and sleep disturbances in the general population from before to during the COVID-19 lockdown: A systematic review and meta-analysis
Authors: Limongi Federica , Siviero Paola , Trevisan Caterina , Noale Marianna , Catalani Filippo , Ceolin Chiara , Conti Silvia , di Rosa Elisa , Perdixi Elena , Remelli Francesca , Prinelli Federica , Maggi Stefania
Published: April 2023

Introduction

This systematic review and meta-analysis aims to explore changes in sleep quality and sleep disturbances in the general population from before to during the COVID-19 lockdown.

Methods

The protocol was registered in PROSPERO (CRD42021256378) and the PRISMA guidelines were followed. The major databases and gray literature were systematically searched from inception to 28/05/2021 to identify observational studies evaluating sleep changes in the general population during the lockdown with respect to the pre-lockdown period. A random effects meta-analysis was undertaken for studies reporting (a) the means of the Pittsburgh Sleep Quality Index (PSQI) global scores or the means of the sleep onset latency (SOL) times (minutes - min) before and during the lockdown, (b) the percentages of poor sleep quality before and during the lockdown, or (c) the percentages of changes in sleep quality. Subgroup analysis by risk of bias and measurement tool utilized was carried out. A narrative synthesis on sleep efficiency, sleep disturbances, insomnia and sleep medication consumption was also performed.

Results

Sixty-three studies were included. A decline in sleep quality, reflected in a pooled increase in the PSQI global scores (standardized mean difference (SMD) = 0.26; 95% CI 0.17–0.34) and in SOL (SMD = 0.38 min; 95% CI 0.30–0.45) were found. The percentage of individuals with poor sleep quality increased during the lockdown (pooled relative risk 1.4; 95% CI 1.24–1.61). Moreover, 57.3% (95% CI 50.01–61.55) of the individuals reported a change in sleep quality; in 37.3% (95% CI 34.27–40.39) of these, it was a worsening. The studies included in the systematic review reported a decrease in sleep efficiency and an increase in sleep disturbances, insomnia, and in sleep medication consumption.

Discussion

Timely interventions are warranted in view of the decline in sleep quality and the increase in sleep disturbances uncovered and their potentially negative impact on health. Further research and in particular longitudinal studies using validated instruments examining the long-term impact of the lockdown on sleep variables is needed.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021256378, identifier CRD42021256378.
Research on the psychologically restorative effects of campus common spaces from the perspective of health
Authors: Guo Weihong , Wen Hongyan , Liu Xiao
Published: April 2023

Contemporary college students are suffering from increasingly serious psychological health problems, such as attention fatigue, psychological stress and negative emotions. A growing body of evidence has revealed that restorative environment design is conducive to psychological health. As the main choice of venue for students’ daily activities, campus common spaces are supposed to be restorative to some extent. Given the above, the author studied 22 common spaces in the South China University of Technology (SCUT) Wushan Campus from the perspective of college students’ behavioral patterns based on theories pertaining to restorative environments, then constructed a structural equation model (SEM) analyzing the psychologically restorative effects exerted by the characteristics of campus common spaces upon college students through a scale design and questionnaire survey. With the analysis of 478 valid questionnaires, the research found that the characteristics of campus common spaces with psychologically restorative effects mainly comprise the architectural environment, landscape environment, rest facilities and activity facilities. Among them, the characteristics of activity facilities and the landscape environment have the greatest impact on psychologically restorative effects, accounting for 33 and 30% of the total effects, respectively; they are followed by those of the architectural environment, which accounts for 21% of the total effects; those of the rest facilities have the least impact, accounting for 16% of the total effects. The research also found that the characteristics of campus common spaces can both directly influence college students’ psychological recovery and produce psychologically restorative effects mediated by college students’ behavioral patterns. The mediation effect of college students’ behavioral patterns accounts for approximately 41% of the total effect of psychological restoration, in which the psychologically restorative effect of dynamic exercise behaviors is 2.5 times that of static leisure behaviors. The research reveals how the characteristics of campus common spaces promote the psychological restoration of college students, and it provides inspiration for healthy environment design in campus common spaces.
Pharmacy-based immunization: a systematic review
Authors: Romero-Mancilla Marisol S. , Mora-Vargas Jaime , Ruiz Angel
Published: April 2023

Background

The coronavirus disease 2019 pandemic has prompted the exploration of new response strategies for such health contingencies in the near future. Over the last 15 years, several pharmacy-based immunization (PBI) strategies have emerged seeking to exploit the potential of pharmacies as immunization, medication sale, and rapid test centers. However, the participation of pharmacies during the last pandemic was very uneven from one country to another, suggesting a lack of consensus on the definition of their roles and gaps between the literature and practice.

Purpose

This study aimed to consolidate the current state of the literature on PBI, document its progress over time, and identify the gaps not yet addressed. Moreover, this study seeks to (i) provide new researchers with an overview of the studies on PBI and (ii) to inform both public health and private organization managers on the range of possible immunization models and strategies.

Methodology

A systematic review of scientific qualitative and quantitative studies on the most important scientific databases was conducted. The Preferred Reporting Items for Systematic Reviews and Meta-analyzes guidelines were followed. Finally, this study discusses the trends, challenges, and limitations on the existing literature on PBI.

Findings

Must studies concluded that PBI is a beneficial strategy for the population, particularly in terms of accessibility and territorial equity. However, the effectiveness of PBI is affected by the economic, political, and/or social context of the region. The collaboration between the public (government and health departments) and private (various pharmacy chains) sectors contributes to PBI's success.

Originality

Unlike previous literature reviews on PBI that compiled qualitative and statistical studies, this study reviewed studies proposing mathematical optimization methods to approach PBI.
Cell Cultures: A Laboratory Tool for Studying Viruses
Authors: Ortega-Soto Elizabeth , Chopin-Doroteo Mario
Published: April 2023

When you hear the word virus, you might think of diseases like rabies, influenza, or COVID-19. Viruses are organisms that must infect living cells to reproduce. Studies done on cells grown in the lab, called cell cultures, have contributed significantly to our understanding of viral diseases and to the development of treatments and vaccines. In the laboratory, cells can be grown in layers or in three-dimensional systems. By infecting cell cultures with viruses, scientists can study various aspects of the infection, such as how the virus gets into the cells or how the cells respond to infection. Knowledge gained from infecting cell cultures with viruses has helped scientists to vastly improve human health!
Evaluation of evapotranspiration models for cucumbers grown under CO2 enriched and HVAC driven greenhouses: A step towards precision irrigation in hyper-arid regions
Authors: Ghiat Ikhlas , Govindan Rajesh , Al-Ansari Tareq
Published: April 2023

Evapotranspiration is considered as one of the most crucial surface fluxes describing the water movement from the land to the atmosphere in the form of evaporation from the soil and transpiration from plants. Several evapotranspiration models exist, but their accuracy is subject to change because of the differences between the underlying assumptions used in their formulation and the conditions of the application at hand. The appropriate selection of an evapotranspiration model is necessary to ensure the accurate estimation of crop water requirements. This work compares between 20 different evapotranspiration models for the estimation of transpiration of cucumber crops grown in a cooling-based greenhouse with CO2 enrichment located in a high solar radiation region. The models are classified into temperature-based, radiation-based, mass transfer-based, and combination models. These models are assessed against direct gas exchange measurements in a greenhouse with cucumber crops. The performance of the models is evaluated using nine statistical indicators to determine the most suitable models for the application under study. Results demonstrate that among the temperature-based models, Schendel and Blaney and Criddle models resulted in the best transpiration prediction, contrary to Hargreaves and Samani which presented the worst performance. Transpiration estimates from Rohwer were the closest and that of Trabert were the furthest to the measured data amongst the other mass-transfer based models. The Abtew model was the best transpiration predicting model, while Priestley and Taylor exhibited the worst performance in the radiation-based model category. The combination-based FAO56 Penman Monteith entailed the best performance among all models and can be considered the best suitable method to estimate transpiration for cucumber crops grown in CO2 enriched and HVAC based greenhouses located in high solar radiation regions. Nonetheless, the parametrization of this model is still crucial and should be considered to achieve better estimates and accurately evaluate the effect of high solar radiation, CO2 enrichment and HVAC cooling for this agricultural greenhouse application.
Conditional Temporal Aggregation for Time Series Forecasting Using Feature-Based Meta-Learning
Authors: Kaltsounis Anastasios , Spiliotis Evangelos , Assimakopoulos Vassilios
Published: April 2023

We present a machine learning approach for applying (multiple) temporal aggregation in time series forecasting settings. The method utilizes a classification model that can be used to either select the most appropriate temporal aggregation level for producing forecasts or to derive weights to properly combine the forecasts generated at various levels. The classifier consists a meta-learner that correlates key time series features with forecasting accuracy, thus enabling a dynamic, data-driven selection or combination. Our experiments, conducted in two large data sets of slow- and fast-moving series, indicate that the proposed meta-learner can outperform standard forecasting approaches.
Reviving the Low-Frequency Response of a Rupestrian Church by Means of FDTD Simulation
Authors: Martellotta Francesco , Liuzzi Stefania , Rubino Chiara
Published: April 2023

Rupestrian churches are spaces obtained from excavation of soft rocks that are frequently found in many Mediterranean countries. In the present paper the church dedicated to Saints Andrew and Procopius, located close to the city of Monopoli in Apulia (Italy) is studied. On-site acoustical measures were made, obtaining a detailed description of the acoustics in the current state pointing out, thanks to a combination of analysis techniques, the presence of significant modal behavior in the low frequencies, causing reverberation time to be about 2 s, four times longer than in the other bands, as well as being strongly dependent on source and receiver position (with variations of about 1 s when source is moved outside the chancel). However, as the church is characterized by significant degradation of surfaces and large amounts of debris cover the floor, the original acoustic conditions can be expected to somewhat differ. Acoustical modelling can be very helpful in grasping the original conditions, but given the small dimensions of the space, conventional geometrical acoustic prediction methods cannot be applied to simulate the low-frequency behavior. Thus, the present paper proposes an application of finite-difference-time-domain (FDTD) computation to simulate the low-frequency behavior and analyze a possible reconstruction of the original state. Results showed that a very good agreement was obtained between predictions and measurements, both in terms of resonance frequencies and reverberation times that differed by less than 5%. Modal response strongly affected the acoustical conditions also in the hypothetical reconstruction of the original state, although the sound field proved to be more uniform than in the current state.
Satellite Attitude Determination Using ADS-B Receiver and MEMS Gyro
Authors: Liu Zhiyong , Zhou Kaixing , Sun Xiucong
Published: April 2023

Automatic dependent surveillance-broadcast (ADS-B) is a very important communication and surveillance technology in air traffic control (ATC). In the future, more and more satellites will carry out ADS-B technology to perform a global coverage. In order to make full use of the resources in the satellite, this paper proposes a solution for satellite three-axis attitude determination using the ADS-B receiver. The principle of ADS-B-based attitude determination is presented first. On this basis, ADS-B-based methods are employed to solve the problem. To achieve a higher attitude determination precision, gyro is combined with the ADS-B receiver using a multiplicative extended Kalman filter (MEKF). Finally, a simulation is carried out and the result is presented.
Plant Growth Regulators for the Cultivation and Vase Life of Geophyte Flowers and Leaves
Authors: Janowska Beata , Andrzejak Roman
Published: April 2023

Geophytes are a very important group among ornamental plants, for which more and more plant growth regulators (PGRs) are being used to improve the plant quality, flowering intensity, and vase life of flowers and leaves. PGRs constitute a large group of naturally occurring or synthetically produced organic chemical compounds. There are many factors that influence the efficiency of PGRs, and the method of their application plays a key role in determining their success. In the case of geophytes, the most common method of application is spraying and soaking the storage organs before planting. This article presents information on the application of PGRs to different species of geophytes, both at the cultivation stage and during the post-harvest treatment of flowers and leaves.