bluuebunny/crossref_metadata_2025 huggingface dataset:
Research and Analysis of Liquid Cooling Heat Dissipation Equipment for Insulated-Gate Bipolar Transistor Modules of Wind Power Converters Based on the Finite Element Analysis Method
Authors: Zhu Xinyu , Hu Xue , Zhang Lixin , Li Wenchun , Gong Lijiao , Yu Wenhao , Wang Haonan , Chen Feng , Zhang Xinwang
Published: October 2024

The primary objective of this study is to develop a simulation model for a liquid cooling plate (LCP) for insulated-gate bipolar transistor (IGBT) modules, with the aim of reducing the operating temperature of wind power converters (WPCs). The initial impetus for this study was the observation that the energy conversion efficiency of a WPC declines when the operating temperature of the IGBT module exceeds a critical threshold. Three LCPs, with and without heat sinks, were modelled under extreme conditions using the finite element simulation method. The effect of the number and height of the fins on the cooling efficacy was evaluated through the simulation and analysis of the LCP model with heat sinks. The results demonstrate that the optimal configuration, comprising five 10 mm fins and 13 10 mm struts, can achieve the following reductions: maximum temperature by 11.4 K, heat dissipation efficiency by 3.33%, pressure drop by 10.6 KPa, and pump power by 31.00%. Moreover, the findings suggest that the number of fins has a significant impact on temperature fluctuations, whereas the height of the fins exerts a markedly significant influence on pressure drop.
Uncovering Hidden Patterns: Approximate Resurgent Resummation from Truncated Series
Authors: Maiezza Alessio , Vasquez Juan Carlos
Published: October 2024

We analyze truncated series generated as divergent formal solutions of non-linear ordinary differential equations. Motivating the study is a specific non-linear, first-order differential equation, which is the basis of the resurgent formulation of renormalized perturbation theory in quantum field theory. We use the Borel–Padé approximant and classical analysis to determine the analytic structure of the solution using the first few terms of its asymptotic series. Afterward, we build an approximant, consistent with the resurgent properties of the equation. The procedure gives an approximate expression for the Borel–Ecalle resummation of the solution useful for practical applications. Connections with other physical applications are also discussed.
A New Hybrid Descent Algorithm for Large-Scale Nonconvex Optimization and Application to Some Image Restoration Problems
Authors: Wang Shuai , Wang Xiaoliang , Tian Yuzhu , Pang Liping
Published: October 2024

Conjugate gradient methods are widely used and attractive for large-scale unconstrained smooth optimization problems, with simple computation, low memory requirements, and interesting theoretical information on the features of curvature. Based on the strongly convergent property of the Dai–Yuan method and attractive numerical performance of the Hestenes–Stiefel method, a new hybrid descent conjugate gradient method is proposed in this paper. The proposed method satisfies the sufficient descent property independent of the accuracy of the line search strategies. Under the standard conditions, the trust region property and the global convergence are established, respectively. Numerical results of 61 problems with 9 large-scale dimensions and 46 ill-conditioned matrix problems reveal that the proposed method is more effective, robust, and reliable than the other methods. Additionally, the hybrid method also demonstrates reliable results for some image restoration problems.
Applying the Simulated Annealing Algorithm to the Set Orienteering Problem with Mandatory Visits
Authors: Lin Shih-Wei , Guo Sirui , Wu Wen-Jie
Published: October 2024

This study addresses the set orienteering problem with mandatory visits (SOPMV), a variant of the team orienteering problem (SOP). In SOPMV, certain critical sets must be visited. The study began by formulating the mathematical model for SOPMV. To tackle the challenge of obtaining a feasible route within time constraints using the original MILP approach, a two-stage mixed-integer linear programming (MILP) model is proposed. Subsequently, a simulated annealing (SA) algorithm and a dynamic programming method were employed to identify the optimal route. The proposed SA algorithm was used to solve the SOP and was compared to other algorithms, demonstrating its effectiveness. The SA was then applied to solve the SOPMV problem. The results indicate that the solutions obtained using SA are superior and more efficient compared to those derived from the original MILP and the two-stage MILP. Additionally, the results reveal that the solution quality deteriorates as the ratio of the set of mandatory visits increases or the maximum allowable travel time decreases. This study represents the first attempt to integrate mandatory visits into SOP, thereby establishing a new research direction in this area. The potential impact of this research is significant, as it introduces new possibilities for addressing complex combinatorial optimization problems.
Adaptive Bi-Encoder Model Selection and Ensemble for Text Classification
Authors: Park Youngki , Shin Youhyun
Published: October 2024

Can bi-encoders, without additional fine-tuning, achieve a performance comparable to fine-tuned BERT models in classification tasks? To answer this question, we present a simple yet effective approach to text classification using bi-encoders without the need for fine-tuning. Our main observation is that state-of-the-art bi-encoders exhibit varying performance across different datasets. Therefore, our proposed approaches involve preparing multiple bi-encoders and, when a new dataset is provided, selecting and ensembling the most appropriate ones based on the dataset. Experimental results show that, for text classification tasks on subsets of the AG News, SMS Spam Collection, Stanford Sentiment Treebank v2, and TREC Question Classification datasets, the proposed approaches achieve performance comparable to fine-tuned BERT-Base, DistilBERT-Base, ALBERT-Base, and RoBERTa-Base. For instance, using the well-known bi-encoder model all-MiniLM-L12-v2 without additional optimization resulted in an average accuracy of 77.84%. This improved to 89.49% through the application of the proposed adaptive selection and ensemble techniques, and further increased to 91.96% when combined with the RoBERTa-Base model. We believe that this approach will be particularly useful in fields such as K-12 AI programming education, where pre-trained models are applied to small datasets without fine-tuning.
An Exploration on Z-Number and Its Properties
Authors: Jiang Guanshuang , Shen Xinyu , Liao Xuefei , Xuan Xiaoqi , Wu Lechen , Zhang Haomin , Li Zhen
Published: October 2024

The Z-number deserves further exploration in uncertain environments to effectively address fuzziness and reliability in practical issues simultaneously. Based on the credibility distribution and conversion rules, we define its expected value, variance, and semi-variance, and demonstrate the feasibility of these calculations by deriving formulas. Moreover, we explore three characteristics inherent in symmetrical Z-numbers. The link between the variance and semi-variance of Z-numbers is discovered and proved. Furthermore, we apply the formulas for expected value and variance to Z-numbers in examples, the results of which validate our proposed formulas. The findings underscore the significance of our study in applying the expected value and variance of fuzzy sets across diverse fields.
Anomaly Detection over Streaming Graphs with Finger-Based Higher-Order Graph Sketch
Authors: Lu Min , Zhang Qianzhen , Zhu Xianqiang
Published: October 2024

A streaming graph is a constantly growing sequence of edges, which forms a dynamic graph that changes with every edge in the stream. An anomalous behavior in a streaming graph can be modeled as an edge or a subgraph that is unusual compared to the rest of the graph. Identifying anomalous behaviors in real time is essential to the early warning of abnormal or notable events. Due to the complexity of the problem, little work has been reported so far to solve the problem. In this paper, we propose Finger-based Higher-order Graph Sketch (FHGS for short), which is an approximate data structure for streaming graphs with linear memory usage, high update speed, and high accuracy and supports both edge and subgraph anomaly detection. FHGS first maps each edge into a matrix based on hash functions, and then counts its frequency in a time window with unique fingerprints for detecting anomalies. Extensive experiments confirm that our approach generate high-quality results compared to baseline methods.
Introducing the Third-Order Fuzzy Superordination Concept and Related Results
Authors: Oros Georgia Irina , Dzitac Simona , Bardac-Vlada Daniela Andrada
Published: October 2024

Third-order fuzzy differential subordination studies were recently initiated by developing the main concepts necessary for obtaining new results on this topic. The present paper introduces the dual concept of third-order fuzzy differential superordination by building on the known results that are valid for second-order fuzzy differential superordination. The outcome of this study offers necessary and sufficient conditions for determining subordinants of a third-order fuzzy differential superordination and, furthermore, for finding the best subordinant for such fuzzy differential superordiantion, when it can be obtained. An example to suggest further uses of the new outcome reported in this work is enclosed to conclude this study.
Optimized Nonlinear PID Control for Maximum Power Point Tracking in PV Systems Using Particle Swarm Optimization
Authors: Zambou Maeva Cybelle Zoleko , Kammogne Alain Soup Tewa , Siewe Martin Siewe , Azar Ahmad Taher , Ahmed Saim , Hameed Ibrahim A.
Published: October 2024

This paper proposes a high-performing, hybrid method for Maximum Power Point Tracking (MPPT) in photovoltaic (PV) systems. The approach is based on an intelligent Nonlinear Discrete Proportional–Integral–Derivative (N-DPID) controller with the Perturb and Observe (P&O) method. The feedback gains derived are optimized by a metaheuristic algorithm called Particle Swarm Optimization (PSO). The proposed methods appear to present adequate solutions to overcome the drawbacks of existing methods despite various weather conditions considered in the analysis, providing a robust solution for dynamic environmental conditions. The results showed better performance and accuracy compared to those encountered in the literature. We also recall that this technique provides a systematic design procedure in the search for the MPPT in photovoltaic (PV) systems that has not yet been documented in the literature to the best of our knowledge.
Degradation of Natural Undaria pinnatifida into Unsaturated Guluronic Acid Oligosaccharides by a Single Alginate Lyase
Authors: Wang Hui , Wen Jiaqi , Ablimit Nuraliya , Deng Kun , Wang Wenzhuo , Jiang Wei
Published: October 2024

Here, we report on a bifunctional alginate lyase (Vnalg7) expressed in Pichia pastoris, which can degrade natural Undaria pinnatifida into unsaturated guluronic acid di- and trisaccharide without pretreatment. The enzyme activity of Vnalg7 (3620.00 U/mL-culture) was 15.81-fold higher than that of the original alg (228.90 U/mL-culture), following engineering modification. The degradation rate reached 52.75%, and reducing sugar reached 30.30 mg/mL after combining Vnalg7 (200.00 U/mL-culture) and 14% (w/v) U. pinnatifida for 6 h. Analysis of the action mode indicated that Vnalg7 could degrade many substrates to produce a variety of unsaturated alginate oligosaccharides (AOSs), and the minimal substrate was tetrasaccharide. Site-directed mutagenesis showed that Glu238, Glu241, Glu312, Arg236, His307, Lys414, and Tyr418 are essential catalytic sites, while Glu334, Glu344, and Asp311 play auxiliary roles. Mechanism analysis revealed the enzymatic degradation pattern of Vnalg7, which mainly recognizes and attacks the third glycosidic linkage from the reducing end of oligosaccharide substrate. Our findings provide a novel alginate lyase tool and a sustainable and commercial production strategy for value-added biomolecules using seaweeds.
Genetic Polymorphisms of Immunity Regulatory Genes and Alopecia Areata Susceptibility in Jordanian Patients
Authors: Alghamdi Mansour , AL-Eitan Laith , Aljamal Hanan , Abu Kharmah Hana
Published: October 2024

Background and Objectives: Alopecia areata (AA) is a tissue-specific immune-mediated disorder that affects hair follicles and the nail apparatus. Due to the collapse of hair follicle immune privilege in AA, hair loss ranges in severity from small, localized patches on the scalp to the loss of entire body hair. Although AA is of uncertain etiology, the disease has a common genetic basis with a number of other autoimmune diseases. Materials and Methods: To identify candidate genes that confer susceptibility to AA in the Jordanian population and further understand the disease background, we performed DNA genotyping using case–control samples of 152 patients and 150 healthy subjects. Results: While no significant result was observed in the ten single-nucleotide polymorphisms (SNPs), CLEC4D rs4304840 variants showed significant associations with AA development within our cohort (p = 0.02). The strongest associations were for the codominant and recessive forms of rs4304840 (p = 0.023 and p = 0.0061, respectively). Conclusions: These findings suggest that CLEC4D gene variants may contribute to AA pathogenesis among Jordanians. Further advanced genetic analysis and functional investigations are required to elucidate the genetic basis of the disease.
Surgical Precision of MICA and Reverdin-Isham Using 3D-Printed Guides: A Cadaveric Study
Authors: Fernández-Ehrling Nadia , Nieto-García Eduardo , Ramirez-Andrés Leonor , Nieto-González Elena , Barrios Carlos , García-Vicente Sergio , Ferrer-Torregrosa Javier
Published: October 2024

Background/Objectives: Hallux valgus is a common foot deformity that requires surgical correction to restore proper alignment. Precision in the osteotomies performed during these surgeries is critical to avoid complications and improve outcomes. However, variability in surgeon experience can negatively affect precision. In this context, advances in 3D printing have enabled the development of customized surgical guides, which may enhance precision and reduce variability among surgeons with different levels of expertise. This study aims to evaluate the effectiveness of a 3D-printed surgical guide in minimally invasive hallux valgus correction, focusing on the accuracy of osteotomies performed by novice surgeons, experienced surgeons, and theoretically trained consultants. Methods: An ex vivo study was performed with 30 cadaveric feet, divided into three groups according to the level of experience of the surgeons: 3D guide group, Master’s students, professionals. All surgeons performed Akin and Reverdin-Isham osteotomies, but the experimental group (the 3D guide group) utilized a customized 3D-printed surgical guide for enhanced precision during these procedures. Radiographic measurements of osteotomy angles and alignment were taken after the interventions, and compared with the planned values. Statistical analyses were conducted to evaluate the variability in the precision of the cuts. Results: The use of the 3D-printed surgical guide significantly reduced angular variability in the experienced group, achieving higher levels of accuracy than experienced surgeons. Effect sizes, which ranged from small to large, indicated a greater impact on angle measurements (η2 = 0.46, p < 0.001); no significant differences were found between the groups in other evaluated parameters. Conclusions: The incorporation of 3D-printed surgical guides in hallux valgus surgery significantly improves osteotomy accuracy, particularly in less experienced surgeons. This suggests that these guides can help standardize procedures, reduce the learning curve, and lower intraoperative complications.
Immediate Loading Full-Arch 3D-Printed Implant-Supported Fixed Rehabilitation: A Case Report with 24-Month Follow-Up
Authors: de Carvalho Formiga Márcio , Fuller Renato , Ardelean Lavinia Cosmina , Shibli Jamil Awad
Published: October 2024

Implant-supported immediate loading full-arch rehabilitation has been documented in the literature. More recently, computed surgical guides have frequently been used since they facilitate planning and performing surgical treatment without the need to raise a flap, thus reducing trauma and morbidity. This case report describes an immediate full-arch, fixed rehabilitation with full loading placed on four commercially available 3D-printed implants, with a 24-month follow-up. The implants were placed with the help of a digitally planned 3D-printed surgical guide. The provisional fixed prosthesis installed immediately was replaced after 3 months. At the time, the soft and hard tissue around the implants appeared stable, without signs of inflammation. The same situation was observed at the 24-month follow-up. Three-dimensional-printed implants seem to be a promising choice in this case. However, further clinical studies with longer follow-up periods are necessary to confirm their efficacy.
Deltopectoral Flap in Head and Neck Reconstruction
Authors: Pecorari Giancarlo , Bissattini Francesco , Gamba Dario , Pizzo Claudia , Motatto Gian Marco , Riva Giuseppe
Published: October 2024

Background and Objectives: The deltopectoral (DP) flap represents a reconstructive option for the head and neck. It is a fasciocutaneous flap raised from the anterior chest wall below the clavicle. Its role partially declined with the arise of free flaps. However, it still remains a valid option in patients that could not undergo a reconstruction with free flaps. The aim of this retrospective study was to evaluate the role of the DP flap in head and neck reconstruction, with a focus on surgical outcomes and complications. Materials and Methods: Thirty-one patients who underwent head and neck reconstruction with DP flap were included in the study. The delayed technique was not used in any procedure to harvest the flap beyond the deltopectoral groove. The patients’ characteristics, the recipient site, the closure of the donor site, and the flap-related complications were recorded. Results: The median time to autonomization of DP flap was 23 days. Postoperative complications were observed in 10 subjects (32.3%). A partial necrosis was seen in five patients (16.1%), while a complete necrosis developed only in one case (3.2%). The diabetes mellitus was the only parameter associated with postoperative complications. In particular, the percentage of necrosis in subjects with or without diabetes was 66.7% and 8.0%, respectively. Conclusions: The DP has a wide range of applications in head and neck reconstruction, and a low complication rate can be observed. The delayed technique does not necessarily need to be applied, and the flap can be extended beyond the deltopectoral groove when necessary. However, patients with diabetes mellitus have a higher risk of postoperative necrosis of the distal portion of the flap.
Age-Related Response to Remimazolam among Older Patients Undergoing Orthopedic Surgery: A Single-Center Prospective Observational Study
Authors: Chae Min Suk , Lee Nuri , Koh Hyun Jung
Published: October 2024

Background and Objectives: Remimazolam, an ultra-short-acting benzodiazepine, is increasingly used in procedural sedation and general anesthesia. It is characterized by rapid onset of action, inactive metabolites, no delay in recovery, and few adverse events. Its hemodynamic and respiratory stability are comparable to other anesthetics, and it is safe in high-risk and geriatric patients. Materials and Methods: This prospective, observational study enrolled 110 geriatric patients (aged 65 to 85 years) scheduled for primary total knee arthroplasty (TKA). The patients were divided into the old (65 to <75 years; n = 52) and the elderly (75 to 85 years; n = 47) geriatric groups. All surgical and anesthetic methods were applied in the same manner, and TKA was performed by one surgeon. Remimazolam was infused at 6 mg/kg/h for 3 min and then at 1 mg/kg/h until the end of surgery The primary study endpoint was the requirement for flumazenil; secondary endpoints were the times to reach a bispectral index (BIS) < 60 and >80, as well as the rate of apnea occurrence. Results: Flumazenil administration was similar in both groups. There were no differences in the time to reach BIS < 60 or the rate of apnea occurrence. Recovery characteristics, including the time to reach BIS > 80 and the achievement of full consciousness, were also comparable between the groups. Conclusions: Remimazolam is well-tolerated in geriatric patients undergoing orthopedic surgery, with minimal age-related differences in response. These results suggest that remimazolam is an appropriate anesthetic for geriatric patients, even with similar dosing strategies. It provides effective anesthetic depth with no significant increases in adverse outcomes during orthopedic surgery.
Combined Effects of Environmental Metals and Physiological Stress on Lipid Dysregulation
Authors: Obeng-Gyasi Emmanuel , Ford Yvonne R.
Published: October 2024

Background: Cardiovascular diseases (CVD) are a leading cause of mortality worldwide, influenced by genetic, environmental, and behavioral factors. This study examines the relationship between heavy metal exposure, chronic physiological stress (allostatic load), and lipid profiles, which are markers of CVD risk, using data from the National Health and Nutrition Examination Survey (NHANES) 2017–2018. Methods: We utilized structural equation modeling (SEM) to explore the associations between blood levels of lead, cadmium, allostatic load (AL), and lipid measures (low-density lipoprotein (LDL), high-density lipoprotein (HDL), and triglycerides). The AL index was derived from cardiovascular, inflammatory, and metabolic biomarkers and categorized into quartiles to identify high-risk individuals, with an index out of 10 subsequently developed. Results: The SEM analysis revealed that both heavy metal exposure and allostatic load are significantly associated with lipid profiles. Higher levels of lead and cadmium were associated with increased LDL and triglycerides, while higher AL scores were linked to increased LDL and triglycerides and decreased HDL levels. Age was also a significant factor, showing positive correlations with LDL and triglycerides, and a negative correlation with HDL. Conclusions: This study underscores the multifactorial nature of CVD, highlighting the combined impact of environmental pollutants and physiological stress on lipid dysregulation. These findings suggest the need for integrated public health strategies that address both environmental exposures and chronic stress to mitigate cardiovascular risk. Further research is warranted to explore the underlying mechanisms and develop targeted interventions.
Landfill Leachate and Coagulants Addition Effects on Membrane Bioreactor Mixed Liquor: Filterability, Fouling, and Pollutant Removal
Authors: Ragio Rodrigo Almeria , Santana Ana Carolina , Subtil Eduardo Lucas
Published: October 2024

Urban wastewater (UWW) and landfill leachate (LL) co-treatment using membrane bioreactors (MBRs) is a valuable method for managing LL in cities. Coagulants can enhance the filterability of mixed liquor (ML), but the assessment of fouling is still needed. This research aimed to investigate the effects of co-treating synthetic wastewater (SWW) and real LL on an MBR, as well as the impact of adding poly-aluminum chloride (PACl) and Tanfloc SG. Cell-ultrafiltration experiments were conducted with four different feeds: synthetic wastewater, co-treatment with LL (20% v/v), and co-treatment with the addition of 30 mg L−1 coagulants (either PACl or Tanfloc). Co-treatment aggravated flux loss and reduced the recovery rate; however, Tanfloc and PACl improved recovery after cleaning (by 11% and 9%, respectively). Co-treatment also increased cake and irrecoverable/irremovable inorganic resistances, though coagulants reduced the latter, despite a lower fit of the Hermia models during the first hour of filtration. Co-treatment reduced the removal efficiencies of almost all pollutants analyzed, with the most significant impacts observed on the organic fraction. Coagulants, particularly Tanfloc, enhanced overall performance by improving flux recovery and reducing irreversibility, thus benefiting membrane lifespan. In conclusion, Tanfloc addition yielded the best results in terms of filterability and pollutant removal.
Differential Hall Effect Metrology for Electrical Characterization of Advanced Semiconductor Layers
Authors: Basol Bulent M. , Joshi Abhijeet
Published: October 2024

Semiconductor layers employed in fabricating advanced node devices are becoming thinner and their electrical properties are diverging from those established for highly crystalline standards. Since these properties also change as a function of depth within the film, accurate carrier profiling solutions are required. The Differential Hall Effect (DHE) technique has the unique capability of measuring mobility and carrier concentration (active carriers) through the depth of a semiconductor film. It comprises making successive sheet resistance and sheet Hall coefficient measurements as the thickness of the electrically active layer at a test region is reduced through successive material removal steps. Difference equations are then used to process the data and plot the desired depth profiles. The fundamentals of DHE were established in 1960s. Recently, the adaption of electrochemical processing for the material removal steps, and the integration of all other functionalities in a Differential Hall Effect Metrology (DHEM) tool, has made this technique more practical and accurate and improved its depth resolution to a sub-nm range. In this contribution, we review the development history of this important technique and present data from recent characterization work carried out on Si, Ge and SiGe layers.
Cyanobacterial Blooms in City Parks: A Case Study Using Zebrafish Embryos for Toxicity Characterization
Authors: Vieira Bruna , Amaral João , Pereira Mário Jorge , Domingues Inês
Published: October 2024

Cyanobacteria are photosynthetic prokaryotes that play an important role in the ecology of aquatic ecosystems. However, they can also produce toxins with negative effects on aquatic organisms, wildlife, livestock, domestic animals, and humans. With the increasing global temperatures, urban parks, renowned for their multifaceted contributions to society, have been largely affected by blooms of toxic cyanobacteria. In this work, the toxicity of two different stages of development of a cyanobacterial bloom from a city park was assessed, evaluating mortality, hatching, development, locomotion (total distance, slow and rapid movements, and path angles) and biochemical parameters (oxidative stress, neurological damage, and tissue damage indicators) in zebrafish embryos/larvae (Danio rerio). Results showed significant effects for the samples with more time of evolution at the developmental level (early hatching for low concentrations (144.90 mg/L), delayed hatching for high concentrations (significant values above 325.90 mg/L), and delayed development at all concentrations), behavioral level (hypoactivity), and biochemical level (cholinesterase (ChE)) activity reduction and interference with the oxidative stress system for both stages of evolution). This work highlights the toxic potential of cyanobacterial blooms in urban environments. In a climate change context where a higher frequency of cyanobacterial proliferation is expected, this topic should be properly addressed by competent entities to avoid deleterious effects on the biodiversity of urban parks and poisoning events of wildlife, pets and people.
Effects of Free or Immobilized Pediococcus acidilactici ORE5 on Corinthian Currants on Gut Microbiome of Streptozotocin-Induced Diabetic Rats
Authors: Prapa Ioanna , Kompoura Vasiliki , Pavlatou Chrysoula , Nelios Grigorios , Mitropoulou Gregoria , Kostomitsopoulos Nikolaos , Plessas Stavros , Bezirtzoglou Eugenia , Karathanos Vaios T. , Yanni Amalia E. , Kourkoutas Yiannis
Published: October 2024

The present study aimed to investigate the effect of a dietary intervention including free or immobilized cells of the presumptive probiotic Pediococcus acidilactici ORE5 on Corinthian currants, a food with beneficial impact in the condition of Type-1 Diabetes Mellitus (T1DM), on the microbiome composition of STZ-induced diabetic rats. Twenty four male Wistar rats were divided into four groups (n = 6 per group): healthy animals, which received the free (H_FP) or the immobilized Pediococcus acidilactici ORE5 cells (H_IPC), and diabetic animals, which received the free (D_FP) or the immobilized Pediococcus acidilactici ORE5 cells(D_IPC) for 4 weeks (109 cfu/day, in all groups). At the end of the dietary intervention, the D_IPC group exerted a lower concentration of the inflammatory cytokine IL-1 beta compared to D_FP. Consumption of immobilized P. acidilactici ORE5 cells on Corinthian currants by diabetic animals led to increased loads of fecal lactobacilli and lower Enterobacteriaceae, coliforms, and Escherichia coli levels, while Actinobacteria phylum, Akkermansia, and Bifidobacterium genera abundances were increased, and fecal lactic acid was elevated. Overall, the results of the present research demonstrated that functional ingredients could ameliorate gut dysbiosis present in T1DM and could be used to design dietary patterns aiming at T1DM management. However, well-designed clinical trials are necessary, in order to confirm the beneficial effects in humans.
Beta-Hydroxybutyric Acid as a Template for the X-ray Powder Diffraction Analysis of Gamma-Hydroxybutyric Acid
Authors: Marabello Domenica , Canepa Carlo , Cioci Alma , Benzi Paola
Published: October 2024

In this paper, we report the possibility of using the X-ray powder diffraction (XRPD) technique to detect gamma-hydroxybutyric acid (GHB) in the form of its sodium salt in different beverages, but because it is not possible to freely buy GHB, beta-hydroxybutyric acid (BHB) and its sodium salt (NaBHB) were used as a model to fine-tune an X-ray diffraction method for the qualitative analysis of the sodium salt of GHB. The method requires only a small quantity of beverage and an easy sample preparation that consists only of the addition of NaOH to the drink and a subsequent drying step. The dry residue obtained can be easily analyzed with XRPD using a single-crystal X-ray diffractometer, which exploits its high sensitivity and allows for very fast pattern collection. Several beverages with different NaBHB:NaOH molar ratios were tested, and the results showed that NaBHB was detected in all drinks analyzed when the NaBHB:NaOH molar ratio was 1:50, using a characteristic peak at very low 2θ values, which also permitted the detection of its presence in complex beverage matrices. Moreover, depending on the amount of NaOH added, shifting and/or splitting of the characteristic NaBHB salt peak was observed, and the origin of this behavior was investigated.
Determination of Antiepileptics in Biological Samples—A Review
Authors: Martinho João , Simão Ana Y. , Barroso Mário , Gallardo Eugenia , Rosado Tiago
Published: October 2024

Epilepsy remains a disease that affects many people around the world. With the development of new drugs to treat this condition, the importance of therapeutic drug monitoring continues to rise and remains a challenge for the medical community. This review article explores recent advances in the detection of antiepileptic drugs across various sample types commonly used for drug monitoring, with a focus on their applications and impact. Some of these new methods have proven to be simpler, greener, and faster, making them easier to apply in the context of therapeutic drug monitoring. Additionally, besides the classic use of blood and its derivatives, there has been significant research into the application of alternative matrices due to their ease of sample collection and capacity to reflect drug behavior in blood. These advances have contributed to increasing the efficacy of therapeutic drug monitoring while enhancing its accessibility to the population.
RETRACTED: Padghan et al. Pyrene-Phosphonate Conjugate: Aggregation-Induced Enhanced Emission, and Selective Fe3+ Ions Sensing Properties. Molecules 2017, 22, 1417
Authors: Padghan Sachin D. , Bhosale Rajesh S. , Bhosale Sidhanath V. , Antolasic Frank , Al Kobaisi Mohammad , Bhosale Sheshanath V.
Published: October 2024

The Molecules Editorial Office retracts the article “Pyrene-Phosphonate Conjugate: Aggregation-Induced Enhanced Emission, and Selective Fe3+ Ions Sensing Properties” [...]
Insights into the Molecular Structure, Stability, and Biological Significance of Non-Canonical DNA Forms, with a Focus on G-Quadruplexes and i-Motifs
Authors: Obara Patrycja , Wolski Paweł , Pańczyk Tomasz
Published: October 2024

This article provides a comprehensive examination of non-canonical DNA structures, particularly focusing on G-quadruplexes (G4s) and i-motifs. G-quadruplexes, four-stranded structures formed by guanine-rich sequences, are stabilized by Hoogsteen hydrogen bonds and monovalent cations like potassium. These structures exhibit diverse topologies and are implicated in critical genomic regions such as telomeres and promoter regions of oncogenes, playing significant roles in gene expression regulation, genome stability, and cellular aging. I-motifs, formed by cytosine-rich sequences under acidic conditions and stabilized by hemiprotonated cytosine–cytosine (C:C+) base pairs, also contribute to gene regulation despite being less prevalent than G4s. This review highlights the factors influencing the stability and dynamics of these structures, including sequence composition, ionic conditions, and environmental pH. Molecular dynamics simulations and high-resolution structural techniques have been pivotal in advancing our understanding of their folding and unfolding mechanisms. Additionally, the article discusses the therapeutic potential of small molecules designed to selectively bind and stabilize G4s and i-motifs, with promising implications for cancer treatment. Furthermore, the structural properties of these DNA forms are explored for applications in nanotechnology and molecular devices. Despite significant progress, challenges remain in observing these structures in vivo and fully elucidating their biological functions. The review underscores the importance of continued research to uncover new insights into the genomic roles of G4s and i-motifs and their potential applications in medicine and technology. This ongoing research promises exciting developments in both basic science and applied fields, emphasizing the relevance and future prospects of these intriguing DNA structures.
Efficient Formaldehyde Gas Sensing Performance via Promotion of Oxygen Vacancy on In-Doped LaFeO3 Nanofibers
Authors: Zhu Lei , Zhang Jiaxin , Wang Jianan , Liu Jianwei , Zhao Wei , Yan Wei
Published: October 2024

Perovskite oxide LaFeO3(LFO) emerges as a potential candidate for formaldehyde (HCHO) detection due to its exceptional electrical conductivity and abundant active metal sites. However, the sensitivity of the LFO sensor needs to be further enhanced. Herein, a series of LaxIn1-xFeO3 (x = 1.0, 0.9, 0.8, and 0.7) nanofibers (LxIn1-xFO NFs) with different ratios of La/In were obtained via the electrospinning method followed by a calcination process. Among all these LxIn1-xFO NFs sensors, the sensor based on the L0.8In0.2FO NFs possessed the maximum response value of 18.8 to 100 ppm HCHO at the operating temperature of 180 °C, which was 4.47 times higher than that based on pristine LFO NFs (4.2). Furthermore, the L0.8In0.2FO NFs sensor also exhibited a rapid response/recovery time (2 s/22 s), exceptional repeatability, and long-term stability. This excellent gas sensing performance of the L0.8In0.2FO NFs can be attributed to the large number of oxygen vacancies induced by the replacement of the A-site La3+ by In3+, the large specific surface area, and the porous structure. This research presents an approach to enhance the HCHO gas sensing capabilities by adjusting the introduced oxygen vacancies through the doping of A-sites in perovskite oxides.
Iodine-Enriched Urea Reduces Volatilization and Improves Nitrogen Uptake in Maize Plants
Authors: Cezar João Victor da Costa , Morais Everton Geraldo de , Lima Jucelino de Sousa , Benevenute Pedro Antônio Namorato , Guilherme Luiz Roberto Guimarães
Published: October 2024

Urea is the primary source of nitrogen (N) used in agriculture. However, it has a high N loss potential through volatilization. Various mechanisms can be employed to reduce N volatilization losses by inhibiting urease. When added to urea, iodine (I) has high potential for this purpose. Thus, this study aimed to determine whether adding I to urea reduces volatilization losses and increases N uptake in maize plants. Maize plants were cultivated in greenhouse conditions for 36 days. Urea treatments were applied at 15 days of testing, including iodine-enriched urea, conventional urea, and no urea application. Additionally, a study concerning N volatilization from urea was conducted using the same treatments under the same environmental conditions. Iodine was incorporated and adhered to urea, at an I concentration of 0.2%, using potassium iodate (KIO3). Under controlled conditions and over a short period of time, it was observed that the application of iodine-enriched urea increased the chlorophyll b content, root N accumulation, and total N accumulation in maize plants compared with conventional urea. Moreover, iodine-enriched urea reduced N losses from volatilization by 11% compared with conventional urea. The reduction in N volatilization correlated positively with the increased chlorophyll b, total chlorophyll, root N accumulation, and total N accumulation favored by the iodine-enriched urea treatment. Our findings demonstrated that adding I to urea is an efficient and promising strategy to reduce N losses and increase N uptake in plants.
Exploring the Efficacy of Alpha-Lipoic Acid in Comorbid Osteoarthritis and Type 2 Diabetes Mellitus
Authors: Halabitska Iryna , Oksenych Valentyn , Kamyshnyi Oleksandr
Published: October 2024

Background/Objectives. The comorbidity of osteoarthritis and type 2 diabetes mellitus poses a complex clinical challenge, complicating patient management due to overlapping pathophysiological mechanisms. This research aims to analyze the exacerbation of clinical symptoms and biochemical markers in patients with OA and T2DM compared to those with OA alone. Methods. We employed various assessment methods to evaluate inflammation, oxidative stress, and glycemic control in both cohorts. This study includes the administration of alpha-lipoic acid (ALA) to patients with comorbid OA and T2DM, monitoring its effects on joint function, inflammatory markers, oxidative stress levels, and glycemic control. Results. The findings indicate that T2DM significantly worsens clinical symptoms and biochemical markers in OA patients. Those with both conditions exhibited elevated indicators of inflammation and oxidative stress compared to OA-only patients. Additionally, correlations among metabolic, psychological, and inflammatory factors were identified. Body mass index emerged as a potential predictor for the deterioration of evaluated parameters. The analysis revealed that ALA administration led to statistically significant improvements in WOMAC pain scores, the Lequesne Algofunctional Index, and the AIMS-P compared to the control group. Conclusions. Further research into ALA’s effects on OA progression in patients with comorbidities is essential for developing personalized treatment approaches.
Study on Vitamin K Levels in Mature Milk of Chinese Lactating Mothers
Authors: Wang Haiyan , Yang Zhenyu , Wang Shuxia , Wu Huwei , Pang Xuehong , Hu Yichun , Yang Xiaoguang
Published: October 2024

Objectives: This study sought to understand the levels of vitamin K in the mature milk of Chinese lactating mothers, thereby providing a foundation for the development of appropriate intake (AI) of vitamin K for infants aged 0–5 months. Methods: Five hundred healthy lactating mothers were selected from the mature milk bank established by the Chinese Maternal and Infant Nutrition and Health Cohort by using a simple random sample procedure. Relevant information about lactating mothers and their infants was obtained by a questionnaire survey. Vitamin K1 and vitamin K2 (MK-4 and MK-7) in mature milk were determined by liquid chromatography–tandem mass spectrometry. Results: The total concentration of vitamin K in mature milk was 4.50 (2.85–6.33) ng/mL. The concentrations of vitamin K1, vitamin K2, MK-4, and MK-7 were 2.81 (1.66–4.39) ng/mL, 1.37 (0.75–2.11) ng/mL, 1.20 (0.58–1.97) ng/mL, and 0.13 (0.08–0.19) ng/mL, respectively. The concentration of vitamin K1 was highest and the concentration of MK-7 was lowest. The concentrations of vitamin K2 and MK-4 in mature milk from the south were significantly higher than those in mature milk from the north. The total vitamin K, vitamin K2, and MK-4 concentrations in mature milk of lactating mothers residing in urban areas were higher than those in rural areas. There was a tendency for the concentration of total vitamin K and vitamin K1 to increase with the mother’s age. Moreover, the concentration of MK-4 in mature milk was highest in summer, followed by spring and winter. The levels of vitamin K1 and MK-4 in mature milk were found to be affected by lactation stage; they were highest at 91–120 days and lowest at 31–60 days. Conclusions: Based on the concentration of vitamin K in mature milk found in this study, it is recommended that the appropriate intake of VK for Chinese infants aged 0–5 months is 3.49 μg/d (2.18 μg/d for VK1 and 1.06 μg/d for VK2).
An Overview of Leishmania In Vitro Cultivation and Implications for Antileishmanial Screenings against Promastigotes
Authors: de Oliveira Filho Virlânio Alves , Garcia Marcus Sávio Araujo , Rosa Leticia Bazilio , Giorgio Selma , Miguel Danilo Ciccone
Published: October 2024

The quest for new drug candidates targeting neglected parasitic diseases has become increasingly urgent over the past decades. Advancements in formulating and optimizing drug delivery systems begin with basic research, including direct assays to evaluate the activity of molecules against parasitic stages maintained in laboratories; i.e., promastigotes. In the context of leishmaniasis, an endemic disease worldwide, the cultivation of Leishmania parasites can vary significantly across different laboratories. Factors such as culture media composition, pH, supplementation, and temperature can lead to varied drug responses in in vitro activity assays. This study aims to compile the parameters used in Leishmania spp. promastigotes cultivation protocols described in scientific articles published in indexed journals over the past ten years. The data reveal a lack of uniformity among Leishmania culture protocols, suggesting a potential bottleneck in comparing the leishmanicidal potential of in vitro drug candidates reported by different research groups. This condition is crucial to consider, because viability/inhibition assays should begin with fully-grown, healthy promastigote cultures capable of homogeneous division, thereby producing more reproducible results.
First Report of Haemaphysalis longicornis (Neumann) in Oklahoma, USA
Authors: Myers Sarah A. , Scimeca Ruth C.
Published: October 2024

Haemaphysalis longicornis (Neumann), the Asian longhorned tick, is a species native to East Asia, but invasive to Australia, New Zealand, and most recently, the United States. It has spread rapidly across the eastern United States after being established in New Jersey in 2017. Aiding this rapid expansion is the ability of this tick to reproduce parthenogenically and feed on diverse host species. In cattle, this tick can cause heavy burdens and act as a vector for the pathogenic hemoprotozoan parasite Theileria orientalis, genotype Ikeda, creating economic losses that impact the cattle industry. Here, we report Asian longhorned ticks, collected from cattle, a dog, and pastures and morphologically identified at the Oklahoma Animal Disease Diagnostic Laboratory as H. longicornis before molecular confirmation through PCR amplification of the cox1 gene. Blood samples from infested cattle were collected and assessed molecularly for the presence of T. orientalis, with no pathogenic DNA detected. This report describes the first record of H. longicornis in Oklahoma and the farthest westward detection of this tick in the United States to date.
From Trypomastigotes to Trypomastigotes: Analyzing the One-Way Intracellular Journey of Trypanosoma cruzi by Ultrastructure Expansion Microscopy
Authors: Tomasina Ramiro , González Fabiana C. , Cabrera Andrés , Basmadjián Yester , Robello Carlos
Published: October 2024

The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas disease, also called American trypanosomiasis. This neglected tropical disease affects millions of individuals across the Americas. To complete its life cycle, T. cruzi parasitizes both vertebrate hosts and its vector, commonly known as the ‘kissing bug’. The parasite’s survival and proliferation strategies are driven by the diverse environments it encounters. Despite being described by Carlos Chagas in 1909, significant knowledge gaps persist regarding the parasite’s various life forms and adaptive capabilities in response to environmental cues. In this study, we employed Ultrastructure Expansion Microscopy to explore the intricate journey of T. cruzi within the host cell. Upon entry into the host cell, trypomastigotes undergo folding, resulting in intermediate forms characterized by a rounded cell body, anterior positioning of basal bodies, and a shortened flagellum. The repositioning of basal bodies and the kinetoplast and the shortening of the flagella mark the culmination of intracellular amastigogenesis. Furthermore, we analyzed intracellular trypomastigogenesis, identifying discrete intermediate forms, including leaf-shaped stages and epimastigote-like forms, which suggests a complex differentiation process. Notably, we did not observe any dividing intracellular epimastigotes, indicating that these may be non-replicative forms within the host cell. Our detailed examination of amastigote cell division revealed semi-closed nuclear mitosis, with mitotic spindle formation independent of basal bodies. This study provides new insights into the morphological and cytoskeletal changes during the intracellular stages of T. cruzi, providing a model for understanding the dynamics of intracellular amastigogenesis and trypomastigogenesis.
Pathways to Alzheimer’s Disease: The Intersecting Roles of Clusterin and Apolipoprotein E in Amyloid-β Regulation and Neuronal Health
Authors: Laslo Alexandru , Laslo Laura , Arbănași Eliza-Mihaela , Ujlaki-Nagi Alexandru-Andrei , Chinezu Laura , Ivănescu Adrian Dumitru , Arbănași Emil-Marian , Cărare Roxana Octavia , Cordoș Bogdan Andrei , Popa Ioana Adriana , Brînzaniuc Klara
Published: October 2024

One of the hallmarks of Alzheimer’s disease (AD) is the deposition of amyloid-β (Aβ) within the extracellular spaces of the brain as plaques and along the blood vessels in the brain, a condition also known as cerebral amyloid angiopathy (CAA). Clusterin (CLU), or apolipoprotein J (APOJ), is a multifunctional glycoprotein that has a role in many physiological and neurological conditions, including AD. The apolipoprotein E (APOE) is a significant genetic factor in AD, and while the primary physiological role of APOE in the brain and peripheral tissues is to regulate lipid transport, it also participates in various other biological processes, having three basic human forms: APOE2, APOE3, and APOE4. Notably, the APOE4 allele substantially increases the risk of developing late-onset AD. The main purpose of this review is to examine the roles of CLU and APOE in AD pathogenesis in order to acquire a better understanding of AD pathogenesis from which to develop targeted therapeutic approaches.