bluuebunny/crossref_metadata_2025 huggingface dataset:
Carbon Sequestration and Contribution of CO2, CH4 and N2O Fluxes to Global Warming Potential from Paddy-Fallow Fields on Mineral Soil Beneath Peat in Central Hokkaido, Japan
Authors: Naser Habib Mohammad , Nagata Osamu , Sultana Sarmin , Hatano Ryusuke
Published: December 2019

Since each greenhouse gas (GHG) has its own radiative capacity, all three gasses (CO2, CH4 and N2O) must be accounted for by calculating the net global warming potential (GWP) in a crop production system. To compare the impact of GHG fluxes from the rice growing and the fallow season on the annual gas fluxes, and their contribution to the GWP and carbon sequestration (CS) were evaluated. From May to April in Bibai (43°18′ N, 141°44′ E), in central Hokkaido, Japan, three rice paddy fields under actual management conditions were investigated to determine CS and the contribution of carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes to GWP. Methane and N2O fluxes were measured by placing the chamber over the rice plants covering four hills and CO2 fluxes from rice plants root free space in paddy fields were taken as an indicator of soil microbial respiration (Rm) using the closed chamber method. Soil CS was calculated as the difference between net primary production (NPP) and loss of carbon (C) through Rm, emission of CH4 and harvest of crop C. Annual cumulative Rm ranged from 422 to 519 g C m−2 yr−1; which accounted for 54.7 to 55.5% of the rice growing season in particular. Annual cumulative CH4 emissions ranged from 75.5 to 116 g C m−2 yr−1 and this contribution occurred entirely during the rice growing period. Total cumulative N2O emissions ranged from 0.091 to 0.154 g N m−2 yr−1 and from 73.5 to 81.3% of the total N2O emissions recorded during the winter-fallow season. The CS ranged from −305 to −365 g C m−2 yr−1, suggesting that C input by NPP may not be compensate for the loss of soil C. The loss of C in the winter-fallow season was much higher (62 to 66%) than in the growing season. The annual net GWP from the investigated paddy fields ranged from 3823 to 5016 g CO2 equivalent m−2 yr−1. Annual GWPCH4 accounted for 71.9 to 86.1% of the annual net GWP predominantly from the rice growing period. These results indicate that CH4 dominated the net GWP of the rice paddy.
Production and Implication of Bio-Activated Organic Fertilizer Enriched with Zinc-Solubilizing Bacteria to Boost up Maize (Zea mays L.) Production and Biofortification under Two Cropping Seasons
Authors: Hussain Azhar , Zahir Zahir Ahmad , Ditta Allah , Tahir Muhammad Usman , Ahmad Maqshoof , Mumtaz Muhammad Zahid , Hayat Khizar , Hussain Shahzad
Published: December 2019

Bio-activated organic fertilizers (BOZ) were produced by enriching the zinc oxide (ZnO)-orange peel waste composite with Zn solubilizing bacteria (ZSB: Bacillus sp. AZ6) in various formulations (BOZ1 (9:1), BOZ2 (8:2), BOZ3 (7:3) and BOZ4 (6:4)). The produced BOZs, along with ZnO, ZnSO4, ZSB were applied to maize crop (Zea mays L.) under field conditions in two different cropping season and the growth, yield, physiology, plant Zn contents and quality of maize were investigated. Results revealed significant variation in the aforementioned parameters with the applied amendments. The BOZ4 performed outclass by exhibiting the highest plant growth, yield, physiology, Zn contents, and quality. On average, an increase of 53%, 49%, 19%, 22%, 10%, 4%, and 30% in plant height was noticed with BOZ4 application over control, ZnO, ZnSO4, BOZ1, BOZ2, BOZ3, and ZSB, respectively. BOZ4 enhanced the dry shoot-biomass 46% than control. Likewise, the photosynthetic rate, transpiration rate, stomatal conductance, chlorophyll contents, carotenoids, and carbonic anhydrase activity were increased by 47%, 42%, 45%, 57%, 17%, and 44%, respectively, under BOZ4 over control in both cropping seasons. However, BOZ4 reduced the electrolyte leakage by 38% as compared to control in both cropping seasons. BOZ4 increased the Zn contents of grain and shoot by 46% and 52%, respectively, while reduced the phytate contents by 73% as compared to control. Application of BOZ4 revealed highest average fat (4.79%), crude protein (12.86%), dry matter (92.03%), fiber (2.87%), gluten (11.925%) and mineral (1.53%) contents, as compared to control. In general, the impact of cropping seasons on maize growth, yield, physiology, Zn contents, and quality were non-significant (with few exceptions). Thus, bio-activation of ZnO with ZSB could serve as an efficient and economical strategy for boosting up the growth, yield, physiological, and quality parameters of maize under field conditions.
Farm-Scale Estimation of Total Nitrogen Content in Surface Paddy Soils by Extraction with Commercially Available Hydrogen Peroxide
Authors: Moritsuka Naoki , Saito Hiroki , Tajima Ryosuke , Takahashi Yukitsugu , Hirai Hideaki
Published: December 2019

We recently proposed a simple method for estimating total nitrogen content in paddy soil. In this method, soil is extracted with a commercial 3% hydrogen peroxide (H2O2) solution at 25 °C for 40 h, and electrical conductivity (EC (H2O2)) of the extract is measured. This study aimed to further evaluate the method’s applicability to soil samples collected at the farm scale by using the original and six additional H2O2 solutions that are locally and commercially available. The results obtained with the original solution indicated that the determination coefficients between EC (H2O2) and total N were statistically significant at all farms examined: Moka, 0.78 (n = 13); Kyoto, 0.50 (n = 16); Kizu, 0.43 (n = 89); and Kawatabi, 0.25 (n = 18). The EC of the tested H2O2 solutions varied from less than 0.05 to 1.4 mS cm−1 because of the addition of different stabilizers. EC (H2O2) values obtained with the less stabilized H2O2 solutions (one from Japan, one from USA, and the analytical grade 6% solution) agreed well with those obtained with the original solution. Thus, the proposed method can be useful for estimating the farm-scale variation in soil total N, provided a H2O2 solution with a low EC (<0.2 mS cm−1) is used for the extraction.
Dried Rumen Digesta Pellet Can Enhance Nitrogen Utilization in Thai Native, Wagyu-Crossbred Cattle Fed Rice Straw Based Diets
Authors: Seankamsorn Anuthida , Cherdthong Anusorn
Published: December 2019

The goal of the current research was to study the effects of a diet of dried rumen digesta pellets (DRDP) on diet utilization, ruminal microorganisms, and ruminal microbes in Thai native, Wagyu-crossbred cattle. Four Thai native, Wagyu-crossbred, beef cattle were assigned to a 4 × 4 Latin square design to supplement DRDP levels at 0, 50, 100, and 150 g/d, respectively. Rice straw intake, total intake, and estimated energy intake varied significantly among the different DRDP levels. Nitrogen intake, apparent N absorption, and apparent N retention were significantly enhanced when compared to the 0 g/d DRDP. DRDP supplementation at 150 g/d produced the greatest apparent digestibility of crude protein compared to the group that was not fed DRDP. Supplementation of DRDP did not alter the population of protozoa, whereas the addition of 150 g DRDP significantly increased the fungal zoospore. Supplementation of DRDP at various levels did not change the concentration of volatile fatty acid (VFA) or the VFA profiles. Thus, DRDP could be an alternative strategic supplement for Thai-native, Wagyu-crossbred cattle in order to enhance N utilization and fungal zoospores.
Effects of Dietary Vegetable Oils on Mammary Lipid-Related Genes in Holstein Dairy Cows
Authors: Vargas-Bello-Pérez Einar , Geldsetzer-Mendoza Carolina , Cancino-Padilla Nathaly , Morales María Sol , Leskinen Heidi , Garnsworthy Philip C. , Loor Juan J. , Romero Jaime
Published: December 2019

This study analyzed effects of vegetable oils fed to dairy cows on abundance of genes related to lipid metabolism in milk somatic cells (MSC). During 63 days, 15 cows were allocated to 3 treatments: a control diet with no added lipid the same diet supplemented with olive oil (OO, 30 g/kg DM) or hydrogenated vegetable oil (HVO, 30 g/kg DM). On days 21, 42 and 63, MSC were obtained from all cows. Relative abundance of genes involved in lipid metabolism in MSC from cows fed control on days 42 and 63 was compared with relative abundance at day 21 to evaluate fold-changes. Those genes without changes over the time were selected to analyze effects of OO and HVO. Compared with control, on day 42, PLIN2 and THRSP were upregulated by OO. Compared with control, on day 21, HVO up regulated ACACA, down regulated FABP3, and on day 63 THRSP and FABP4 were down regulated. Dietary oil supplementation (3% DM) had a modest nutrigenomic effect on different biological functions such as acetate and FA activation and intra-cellular transport, lipid droplet formation, and transcription regulation in MSC.
Endoplasmic Reticulum Stress-Induced Resistance to Doxorubicin Is Reversed by Mulberry Leaf Polyphenol Extract in Hepatocellular Carcinoma through Inhibition of COX-2
Authors: Yang Mon-Yuan , Wu Cheng-Hsun , Hung Tung-Wei , Wang Chau-Jong
Published: December 2019

Mulberry (Morus alba L.) leaves are used in Chinese medicine to treat metabolic disorders. Mulberry leaf polyphenol extracts (MLPE) have recently been shown to exhibit anticancer properties. Endoplasmic reticulum (ER) stress represents a pivotal obstacle in solid tumors, resulting in the antiapoptosis of tumor cells and drug resistance. In this study, pretreatment with the ER stress inducer tunicamycin (TM) attenuated the percentage of apoptosis induced by doxorubicin (DOX). Cotreatment with tunicamycin and MLPE reversed apoptosis induced by DOX. Simultaneously, induction of ER stress with tunicamycin resulted in an increased expression of Cyclooxygenase 2 (COX-2) and Glucose-regulated protein (GRP78) concomitant with the activation of p38 MAPK/PI3K/Akt in HepG2 cells. Furthermore, the suppression of ER stress with celecoxib or p38 MAPK inhibitor successfully recovered DOX-induced apoptosis. Consistent with the inhibition of COX-2 or p38 MAPK, copretreatment with TM and MLPE drastically recovered cytotoxicity and caspase-3 activation in the presence of DOX. These results reveal that MLPE reduces ER stress-induced resistance to DOX in hepatocellular carcinoma (HCC) cells through downregulation of COX-2- or p38 MAPK-mediated PI3K/Akt pathway.
Comparison of Current–Voltage Response to Diagnostic X-rays of Five Light-Emitting Diode Strips
Authors: Damulira Edrine , Yusoff Muhammad , Sulaiman Suharti , Zulkafli Nur , Zulkifli Nur , Shakir Nur , Zainun Mohamad , Omar Ahmad , Taib Nur , Ya Ali Nik
Published: December 2019

Light-emitting diodes (LEDs) have miscellaneous applications owing to their low cost, small size, flexibility, and commercial availability. Furthermore, LEDs have dual applicability as light emitters and detectors. This study explores the current–voltage (C–V) response of LED strips exposed to diagnostic x-rays. Cold white, warm white, red, green, and blue LED strip colors were tested. Each strip consisted of 12 LED chips and was connected to a multimeter. The variable diagnostic x-ray parameters evaluated were kilovoltage peak (kVp), milliampere-seconds (mAs), and source-to-image distance (SID). The radiation dose was also measured using a dosimeter simultaneously exposed to x-rays perpendicularly incident on the strips. Lastly, the consistency of C–V responses, and any possible degradation after 1–2 months was also analyzed. Each LED strip color was ranked according to its C–V response in each of the investigated parameters. The LED strip color with the best cumulative rank across all the tested parameters was then examined for reproducibility. Our findings revealed that the C–V responses of LED strips are (a) generally low but measurable, (b) inconsistent and fluctuating as a consequence of kVp variations, (c) positively correlated to mAs, (d) negatively correlated to SID, and (e) positively correlated to dose. Overall results suggested cold white LED strip as most feasible for x-ray detection—in comparison to examined colors. Additionally, the reproducibility study using the cold white LED strip found a similar trend of C–V response to all variables except kVp. Outcomes indicate that LED strips have the potential to be exploited for detecting low dose (~0–100 mGy) diagnostic x-rays. However, future studies should be carried out to increase the low C–V signal.
Repetition-Based Cooperative Broadcasting for Vehicular Ad Hoc Networks Under the Rayleigh Fading Channel
Authors: Yang Fan , Sun Yanglong , Zou Sai , Luo Xiongbiao
Published: December 2019

In vehicular ad hoc networks (VANETs), safety applications require a reliable and delay-guaranteed broadcasting service to disseminate safety messages. However, channel fading and the high mobility of vehicles make it very challenging for a broadcasting scheme to meet the strict service demands of safety messages. On the other hand, cooperative retransmission is effective in mitigating wireless channel impairments by utilizing the broadcast nature of wireless channels. Therefore, this paper proposes a repetition-based cooperative broadcasting (RBCB) scheme for safety messages in VANETs. The proposed scheme enables the selected helper vehicles to perform the cooperative rebroadcasting along with the source vehicle during the source vehicle’s slot in order to increase the transmission reliability of safety messages and reduce rebroadcasting times. The performance of RBCB scheme is mathematically analyzed in terms of packet delivery probability and average packet delay under the Rayleigh fading channel. Moreover, extensive simulations are conducted to evaluate the performance of RBCB scheme. Both mathematical analysis and simulation results demonstrate that RBCB scheme significantly improves the packet delivery probability and decreases the average packet delay.
Study of Thermal Effect in the Interaction of Nanosecond Capillary Discharge Extreme Ultraviolet Laser with Copper
Authors: Cui Huaiyu , Zhao Yongpeng , Khan Muhammad Usman , Zhao Dongdi , Fan Zhigang
Published: December 2019

Interaction of Extreme Ultraviolet (EUV) laser with matters is an attractive subject since novel phenomena always occur under the effect of high energy photons. In this paper, the thermal effect involved in the interaction of a capillary discharge 46.9 nm laser with copper was studied theoretically and experimentally. The temperature variation of the laser-irradiated region of copper was calculated. According to the results, the copper surface was ablated obviously and presented the trace of melting, evaporation, and resolidification, which suggested the thermal effect occurred on the surface during the laser irradiation.
A Novel Fault Diagnosis Method for High-Temperature Superconducting Field Coil of Superconducting Rotating Machine
Authors: Song Seunghyun , Ko Tae Kuk , Choi Yojong , Lee SangGap
Published: December 2019

In this paper, a new method is presented for sensitive quench detection in high-temperature superconductor (HTS) rotating machinery. The normal zone propagation velocity of an HTS is about 1000 times slower than that of a low-temperature superconductor. Therefore, the propagation of normal zone resistance, which occurs when the HTS transits from the superconducting state to the normal state, is also slower. Thus, it is difficult to detect the abnormal signals by voltage measurement using voltage taps. Moreover, the monitoring signal includes noise generated by interaction between the HTS rotating machinery and the industrial environment. Therefore, when quenching occurs in the HTS rotating machinery, a thermal runaway occurs in the hot spot. Furthermore, the magnetic energy stored in the HTS coil can damage the machinery. For these reasons, a new method is proposed for sensitive quench detection that reduces the noise generated from the power supply and from the HTS rotating machinery, using both an RLC resonance circuit and fast Fourier transform analysis.
Evaluation of Performance and Challenges of Use of Waste Materials in Pavement Construction: A Critical Review
Authors: Jamshidi Ali , White Greg
Published: December 2019

Paved surfaces must reliably bear heavy loads, often under challenging environmental and geotechnical conditions. These requirements are addressed through the use of high-quality, newly produced materials in pavement design. However, in remote locations, newly produced materials are often expensive or unavailable, making waste or alternative materials more attractive. Waste materials can be used in their natural condition but are more commonly stabilised or otherwise improved to meet performance targets. However, this practice can incorporate unwarranted risk into pavement design solutions. The decision to use waste materials in a pavement is a balance between technical risk, maintenance liability, available materials, environmental emissions and capital cost. This study reviews the use of waste materials in pavement design and construction. Reclaimed asphalt pavement (RAP) materials and processed waste plastic for pavement construction are considered. Additionally, blast furnace slag (BFS) and waste glass in pavement construction are evaluated. This review focuses on the effects of alternative materials on the properties of asphalt pavement. The results indicate that RAP is acceptable as an alternative material, while BFS, waste plastic and waste glass can be used under specific conditions. Also, the current and future challenges for the use of waste materials in the pavement industry are discussed.
Turbulence, Low-Level Jets, and Waves in the Tyrrhenian Coastal Zone as Shown by Sodar
Authors: Petenko Igor , Casasanta Giampietro , Bucci Simone , Kallistratova Margarita , Sozzi Roberto , Argentini Stefania
Published: December 2019

The characteristics of the vertical and temporal structure of the coastal atmospheric boundary layer are variable for different sites and are often not well known. Continuous monitoring of the atmospheric boundary layer was carried out close to the Tyrrhenian Sea, near Tarquinia (Italy), in 2015–2017. A ground-based remote sensing instrument (triaxial Doppler sodar) and in situ sensors (meteorological station, ultrasonic anemometer/thermometer, and net radiometer) were used to measure vertical wind velocity profiles, the thermal structure of the atmosphere, the height of the turbulent layer, turbulent heat and momentum fluxes in the surface layer, atmospheric radiation, and precipitation. Diurnal alternation of the atmospheric stability types governed by the solar cycle coupled with local sea/land breeze circulation processes is found to be variable and is classified into several main regimes. Low-level jets (LLJ) at heights of 100–300 m above the surface with maximum wind speed in the range of 5–18 m s−1 occur in land breezes, both during the night and early in the morning. Empirical relationships between the LLJ core wind speed characteristics and those near the surface are obtained. Two separated turbulent sub-layers, both below and above the LLJ core, are often observed, with the upper layer extending up to 400–600 m. Kelvin–Helmholtz billows associated with internal gravity–shear waves occurring in these layers present opposite slopes, in correspondence with the sign of vertical wind speed gradients. Our observational results provide a basis for the further development of theoretical and modelling approaches, taking into account the wave processes occurring in the atmospheric boundary layer at the land–sea interface.
RePLaT-Chaos: A Simple Educational Application to Discover the Chaotic Nature of Atmospheric Advection
Authors: Haszpra Tímea
Published: December 2019

Large-scale atmospheric pollutant spreading via volcano eruptions and industrial accidents may have serious effects on our life. However, many students and non-experts are generally not aware of the fact that pollutant clouds do not disperse in the atmosphere like dye blobs on clothes. Rather, an initially compact pollutant cloud soon becomes strongly stretched with filamentary and folded structure. This is the result of the chaotic behaviour of advection of pollutants in 3-D flows, i.e., the advection dynamics of pollutants shows the typical characteristics such as sensitivity to the initial conditions, irregular motion, and complicated but well-organized (fractal) structures. This study presents possible applications of a software called RePLaT-Chaos by means of which the characteristics of the long-range atmospheric spreading of volcanic ash clouds and other pollutants can be investigated in an easy and interactive way. This application is also a suitable tool for studying the chaotic features of the advection and determines two quantities which describe the chaoticity of the advection processes: the stretching rate quantifies the strength of the exponential stretching of pollutant clouds; and the escape rate characterizes the rate of the rapidity by which the settling particles of a pollutant cloud leave the atmosphere.
The Contribution Rate of Driving Factors and Their Interactions to Temperature in the Yangtze River Delta Region
Authors: Zhou Cheng , Zhu Nina , Xu Jianhua , Yang Dongyang
Published: December 2019

Complex temperature processes are the coupling results of natural and human processes, but few studies focused on the interactive effects between natural and human systems. Based on the dataset for temperature during the period of 1980–2012, we analyzed the complexity of temperature by using the Correlation Dimension (CD) method. Then, we used the Geogdetector method to examine the effects of factors and their interactions on the temperature process in the Yangtze River Delta (YRD). The main conclusions are as follows: (1) the temperature rose 1.53 °C; and, among the dense areas of population and urban, the temperature rose the fastest. (2) The temperature process was more complicated in the sparse areas of population and urban than in the dense areas of population and urban. (3) The complexity of temperature dynamics increased along with the increase of temporal scale. To describe the temperature dynamic, at least two independent variables were needed at a daily scale, but at least three independent variables were needed at seasonal and annual scales. (4) Each driving factor did not work alone, but interacted with each other and had an enhanced effect on temperature. In addition, the interaction between economic activity and urban density had the largest influence on temperature.
Enhancing Potential of Trimethylamine Oxide on Atmospheric Particle Formation
Authors: Myllys Nanna , Ponkkonen Tuomo , Chee Sabrina , Smith James
Published: December 2019

The role of an oxidation product of trimethylamine, trimethylamine oxide, in atmospheric particle formation is studied using quantum chemical methods and cluster formation simulations. Molecular-level cluster formation mechanisms are resolved, and theoretical results on particle formation are confirmed with mass spectrometer measurements. Trimethylamine oxide is capable of forming only one hydrogen bond with sulfuric acid, but unlike amines, trimethylamine oxide can form stable clusters via ion–dipole interactions. That is because of its zwitterionic structure, which causes a high dipole moment. Cluster growth occurs close to the acid:base ratio of 1:1, which is the same as for other monoprotic bases. Enhancement potential of trimethylamine oxide in particle formation is much higher than that of dimethylamine, but lower compared to guanidine. Therefore, at relatively low concentrations and high temperatures, guanidine and trimethylamine oxide may dominate particle formation events over amines.
Alzheimer’s Disease Pharmacotherapy in Relation to Cholinergic System Involvement
Authors: Stanciu Gabriela Dumitrita , Luca Andrei , Rusu Razvan Nicolae , Bild Veronica , Beschea Chiriac Sorin Ioan , Solcan Carmen , Bild Walther , Ababei Daniela Carmen
Published: December 2019

Alzheimer’s disease, a major and increasing global health challenge, is an irreversible, progressive form of dementia, associated with an ongoing decline of brain functioning. The etiology of this disease is not completely understood, and no safe and effective anti-Alzheimer’s disease drug to prevent, stop, or reverse its evolution is currently available. Current pharmacotherapy concentrated on drugs that aimed to improve the cerebral acetylcholine levels by facilitating cholinergic neurotransmission through inhibiting cholinesterase. These compounds, recognized as cholinesterase inhibitors, offer a viable target across key sign domains of Alzheimer’s disease, but have a modest influence on improving the progression of this condition. In this paper, we sought to highlight the current understanding of the cholinergic system involvement in Alzheimer’s disease progression in relation to the recent status of the available cholinesterase inhibitors as effective therapeutics.
Mercury and Alzheimer’s Disease: Hg(II) Ions Display Specific Binding to the Amyloid-β Peptide and Hinder Its Fibrillization
Authors: Wallin Cecilia , Friedemann Merlin , Sholts Sabrina B. , Noormägi Andra , Svantesson Teodor , Jarvet Jüri , Roos Per M. , Palumaa Peep , Gräslund Astrid , Wärmländer Sebastian K. T. S.
Published: December 2019

Brains and blood of Alzheimer’s disease (AD) patients have shown elevated mercury concentrations, but potential involvement of mercury exposure in AD pathogenesis has not been studied at the molecular level. The pathological hallmark of AD brains is deposition of amyloid plaques, consisting mainly of amyloid-β (Aβ) peptides aggregated into amyloid fibrils. Aβ peptide fibrillization is known to be modulated by metal ions such as Cu(II) and Zn(II). Here, we study in vitro the interactions between Aβ peptides and Hg(II) ions by multiple biophysical techniques. Fluorescence spectroscopy and atomic force microscopy (AFM) show that Hg(II) ions have a concentration-dependent inhibiting effect on Aβ fibrillization: at a 1:1 Aβ·Hg(II) ratio only non-fibrillar Aβ aggregates are formed. NMR spectroscopy shows that Hg(II) ions interact with the N-terminal region of Aβ(1–40) with a micromolar affinity, likely via a binding mode similar to that for Cu(II) and Zn(II) ions, i.e., mainly via the histidine residues His6, His13, and His14. Thus, together with Cu(II), Fe(II), Mn(II), Pb(IV), and Zn(II) ions, Hg(II) belongs to a family of metal ions that display residue-specific binding interactions with Aβ peptides and modulate their aggregation processes.
Midday Napping and Successful Aging in Older People Living in the Mediterranean Region: The Epidemiological Mediterranean Islands Study (MEDIS)
Authors: Foscolou Alexandra , D’Cunha Nathan M. , Naumovski Nenad , Tyrovolas Stefanos , Rallidis Loukianos , Matalas Antonia-Leda , Polychronopoulos Evangelos , Sidossis Labros S. , Panagiotakos Demosthenes
Published: December 2019

The aim of the present study was to investigate the association between midday napping, sleeping hours, and successful aging among 2564 older (65+ years) individuals living in the insular Mediterranean region. Anthropometric, clinical, and socio-demographic characteristics, dietary habits, and lifestyle parameters were derived through standard procedures, while successful aging was evaluated using the validated Successful Aging Index (SAI; range 0–10). Of the 2564 participants, 74% reported midday napping. The SAI score was 2.9/10 for non-midday nappers vs. 3.5/10 for midday nappers (p = 0.001). Midday nappers were more likely to be physically active (p = 0.01) and to have higher adherence to the Mediterranean diet (p = 0.02) compared to non-midday nappers. In a fully adjusted model, midday nappers had 6.7% higher SAI score compared to the rest (p < 0.001), and the effect of midday napping was more prominent among males and participants 80+ years of age. Further analysis indicated a significant U-shaped trend between sleeping hours/day and SAI score (p < 0.001), with 8–9 h total of sleep/day, midday napping included, proposed as optimal in achieving the best SAI score. Midday napping seems to be a beneficial habit that should be promoted and encouraged in older people.
Crisis of Meaning and Subjective Well-Being: The Mediating Role of Resilience and Self-Control among Gifted Adults
Authors: Vötter Bernadette
Published: December 2019

Meaning in life is positively associated with mental and physical health, while a crisis of meaning is a painful existential state that is defined as a perceived lack of meaning. An earlier study has shown that academically high-achieving adults mostly experience existential fulfilment, while intellectually gifted adults have a disproportionally high risk of suffering from a crisis of meaning, which can weaken their potential fulfilment in life. To uncover the underlying mechanisms of how an existential crisis affects gifted adults’ mental health, this study examines the longitudinal relationship between crisis of meaning and subjective well-being via two mediators: self-control and resilience. A multiple mediation model was tested with longitudinal data (two times of measurement) of two gifted groups: intellectually gifted adults (HIQ; N = 100; 55% female) and academically high-achieving adults (HAA; N = 52; 29% female). Results suggest group differences: HIQ had higher crisis of meaning and lower self-control than the HAA. HIQ’s resilience (but not their self-control) and HAA’s self-control (but not their resilience) mediated the relationship between crisis of meaning and subjective well-being. These findings give initial insights about the distinct psychological needs of gifted adults and their different paths toward subjective well-being. These insights can be applied in future giftedness research, talent development programs, or counseling to support gifted individuals in living up to their potential. Thus, HIQ could benefit particularly from supporting their ability to cope with adversity, while HAA could benefit particularly from strengthening their willpower to modify undesired emotions, behaviors, and desires.
Characterization of JAK1 Pseudokinase Domain in Cytokine Signaling
Authors: Raivola Juuli , Haikarainen Teemu , Silvennoinen Olli
Published: December 2019

The Janus kinase-signal transducer and activator of transcription protein (JAK-STAT) pathway mediates essential biological functions from immune responses to haematopoiesis. Deregulated JAK-STAT signaling causes myeloproliferative neoplasms, leukaemia, and lymphomas, as well as autoimmune diseases. Thereby JAKs have gained significant relevance as therapeutic targets. However, there is still a clinical need for better JAK inhibitors and novel strategies targeting regions outside the conserved kinase domain have gained interest. In-depth knowledge about the molecular details of JAK activation is required. For example, whether the function and regulation between receptors is conserved remains an open question. We used JAK-deficient cell-lines and structure-based mutagenesis to study the function of JAK1 and its pseudokinase domain (JH2) in cytokine signaling pathways that employ JAK1 with different JAK heterodimerization partner. In interleukin-2 (IL-2)-induced STAT5 activation JAK1 was dominant over JAK3 but in interferon-γ (IFNγ) and interferon-α (IFNα) signaling both JAK1 and heteromeric partner JAK2 or TYK2 were both indispensable for STAT1 activation. Moreover, IL-2 signaling was strictly dependent on both JAK1 JH1 and JH2 but in IFNγ signaling JAK1 JH2 rather than kinase activity was required for STAT1 activation. To investigate the regulatory function, we focused on two allosteric regions in JAK1 JH2, the ATP-binding pocket and the αC-helix. Mutating L633 at the αC reduced basal and cytokine induced activation of STAT in both JAK1 wild-type (WT) and constitutively activated mutant backgrounds. Moreover, biochemical characterization and comparison of JH2s let us depict differences in the JH2 ATP-binding and strengthen the hypothesis that de-stabilization of the domain disturbs the regulatory JH1-JH2 interaction. Collectively, our results bring mechanistic understanding about the function of JAK1 in different receptor complexes that likely have relevance for the design of specific JAK modulators.
Enhanced Photocatalytic Activity of Semiconductor Nanocomposites Doped with Ag Nanoclusters Under UV and Visible Light
Authors: González-Rodríguez Jorge , Fernández Lucía , Bava Yanina B. , Buceta David , Vázquez-Vázquez Carlos , López-Quintela Manuel Arturo , Feijoo Gumersindo , Moreira Maria Teresa
Published: December 2019

Emerging contaminants (ECs) represent a wide range of compounds, whose complete elimination from wastewaters by conventional methods is not always guaranteed, posing human and environmental risks. Advanced oxidation processes (AOPs), based on the generation of highly oxidizing species, lead to the degradation of these ECs. In this context, TiO2 and ZnO are the most widely used inorganic photocatalysts, mainly due to their low cost and wide availability. The addition of small amounts of nanoclusters may imply enhanced light absorption and an attenuation effect on the recombination rate of electron/hole pairs, resulting in improved photocatalytic activity. In this work, we propose the use of silver nanoclusters deposited on ZnO nanoparticles (ZnO–Ag), with a view to evaluating their catalytic activity under both ultraviolet A (UVA) and visible light, in order to reduce energetic requirements in prospective applications on a larger scale. The catalysts were produced and then characterized by scanning electron microscopy (SEM), X-ray diffractometry (XRD) and inductively coupled plasma-optical emission spectrometry (ICP-OES). As proof of concept of the capacity of photocatalysts doped with nanoclusters, experiments were carried out to remove the azo dye Orange II (OII). The results demonstrated the high photocatalytic efficiency achieved thanks to the incorporation of nanoclusters, especially evident in the experiments performed under white light.
A Comparative Evaluation of Physicochemical Properties and Photocatalytic Efficiencies of Cerium Oxide and Copper Oxide Nanofluids
Authors: M. Rashmi , R. Padmanaban , Karthikeyan Vaithinathan , Roy Vellaisamy A. L. , Gopalan Anantha-Iyengar , Saianand Gopalan , Kim Wha-Jung , Kannan Venkatramanan
Published: December 2019

Copper oxide (CuO) and cerium oxide (CeO2) of various concentrations have been prepared through an ultrasonically assisted dispersion of CuO and CeO2 nanoparticles (NPs) in which water and nanofluids (NFs) were formulated. The morphological properties of the CuO and CeO2 NPs are reported. Few of the physicochemical properties that can influence the photocatalytic activities of the NFs are evaluated, such as viscosity, activation energy, density, thermal conductivity, electrical conductivity, alternating current (AC) conductivity, pH, stability, refractive index and optical band gap of the CuO and CeO2 NFs. Viscosity studies have been made at four different temperatures (303 K, 308 K, 313 K and 318 K) and the activation energy is calculated and compared between the CuO and CeO2 NFs. The thermal conductivity of the two NFs is calculated and compared. Electrical conductivity is measured for CuO and CeO2 NFs using an impedance analyzer at different frequencies at 303 K. The dielectric constant and AC conductivity were studied. The electrical conductivity and pH of the prepared NFs are measured and the results are compared. The stability of the NFs is determined from Zeta potential values obtained from dynamic light scattering measurements. UV-Visible diffuse reflectance measurements were used to deduce the optical bandgap of the respective metal oxide NPs in the NFs. The photocatalytic efficiencies of the CuO NFs and CeO2 NFs were evaluated using methylene blue (MB) as the model dye. The rate constant for the photodegradation of MB was higher for CuO NF as compared CeO2 NF and also higher than simple NPs-based photocatalysts. A plausible explanation for the role of NFs over the simple NPs-based photocatalytic solution is presented.
Photocatalytic Oxidation of Methyl Tert-Butyl Ether in Presence of Various Phase Compositions of TiO2
Authors: Šihor Marcel , Reli Martin , Vaštyl Michal , Hrádková Květoslava , Matějová Lenka , Kočí Kamila
Published: December 2019

MTBE (methyl tert-butyl ether) represents a rising threat to the environment, especially drinking water, and its effective removal (with all by-products) is necessary. Even a very low concentration of MTBE makes the water undrinkable; therefore, an effective treatment has to be developed. This work is focused on MTBE photocatalytic oxidation in presence of various TiO2 photocatalysts with different phase composition prepared by different methods. It was confirmed the phase composition of TiO2 had the most significant influence on the photocatalytic degradation of MTBE. The rutile phase more easily reduces adsorbed oxygen by photogenerated electrons to superoxide radical, supporting separation of charge carriers. The presence and concentrations of by-products have to be taken into account as well. The conversion of total organic carbon (TOC) was used for the comparison, 40% of TOC was removed after 1 h of irradiation in presence of TiO2–ISOP–C/800 photocatalyst composed of anatase and rutile phase.
Highly Efficient Biphasic System for the Synthesis of Alkyl Lactates in the Presence of Acidic Ionic Liquids
Authors: Dorosz Urszula , Barteczko Natalia , Latos Piotr , Erfurt Karol , Pankalla Ewa , Chrobok Anna
Published: December 2019

Alkyl lactates are produced from lactic acid via esterification, and are used in the production of plastics, paints, solvents and detergents. In the pursuit of an inexpensive, industry-suitable catalyst for this reaction, the application of protic ionic liquids based on nitrogen base and sulphuric acid is proposed. The ionic liquid was synthesised via a simple reaction of triethylamine and a threefold molar excess of sulphuric acid. Water was added to remove the heat of the reaction. Next, the reaction conditions for the model esterification of 2-ethylhexanol with lactic acid without additional solvent were optimised. Exceptionally mild conditions, i.e., a twofold molar excess of alcohol to lactic acid with the addition of an ionic liquid in a catalytic amount (15 mol%) at 60 °C, resulted in high yields of ethyl and 2-ethylhexyl lactates (96–97%). The driving force of this reaction is the production of a biphasic system with immiscible ester during the reaction. This phenomenon makes it possible to overcome the reaction equilibrium. Using an inexpensive ionic liquid, which could be recycled up to five times without diminution in conversion or selectivity, leads to both a greener and a more economically-viable process.
miR-9-5p Exerts a Dual Role in Cervical Cancer and Targets Transcription Factor TWIST1
Authors: Babion Iris , Jaspers Annelieke , van Splunter Annina P. , van der Hoorn Iris A.E. , Wilting Saskia M. , Steenbergen Renske D.M.
Published: December 2019

Squamous cell carcinoma (SCC) and adenocarcinoma (AC) represent the major cervical cancer histotypes. Both histotypes are caused by infection with high-risk HPV (hrHPV) and are associated with deregulated microRNA expression. Histotype-dependent expression has been observed for miR-9-5p, showing increased expression in SCC and low expression in AC. Here, we studied the regulation and functionality of miR-9-5p in cervical SCCs and ACs using cervical tissue samples and hrHPV-containing cell lines. Expression and methylation analysis of cervical tissues revealed that low levels of miR-9-5p in ACs are linked to methylation of its precursor genes, particularly miR-9-1. Stratification of tissue samples and hrHPV-containing cell lines suggested that miR-9-5p depends on both histotype and hrHPV type, with higher expression in SCCs and HPV16-positive cells. MiR-9-5p promoted cell viability and anchorage independence in cervical cancer cell lines SiHa (SCC, HPV16) and CaSki (metastasized SCC, HPV16), while it played a tumor suppressive role in HeLa (AC, HPV18). TWIST1, a transcription factor involved in epithelial-to-mesenchymal transition (EMT), was established as a novel miR-9-5p target. Our results show that miR-9-5p plays a dual role in cervical cancer in a histotype- and hrHPV type-dependent manner. MiR-9-5p mediated silencing of TWIST1 suggests two distinct mechanisms towards EMT in cervical cancer.
CCL11 Differentially Affects Post-Stroke Brain Injury and Neuroregeneration in Mice Depending on Age
Authors: Lieschke Simone , Zechmeister Bozena , Haupt Matteo , Zheng Xuan , Jin Fengyan , Hein Katharina , Weber Martin S. , Hermann Dirk M. , Bähr Mathias , Kilic Ertugrul , Doeppner Thorsten R.
Published: December 2019

CCL11 has recently been shown to differentially affect cell survival under various pathological conditions including stroke. Indeed, CCL11 promotes neuroregeneration in neonatal stroke mice. The impact of CCL11 on the adult ischemic brain, however, remains elusive. We therefore studied the effect of ectopic CCL11 on both adolescent (six-week) and adult (six-month) C57BL6 mice exposed to stroke. Intraperitoneal application of CCL11 significantly aggravated acute brain injury in adult mice but not in adolescent mice. Likewise, post-stroke neurological recovery after four weeks was significantly impaired in adult mice whilst CCL11 was present. On the contrary, CCL11 stimulated gliogenesis and neurogenesis in adolescent mice. Flow cytometry analysis of blood and brain samples revealed a modification of inflammation by CCL11 at subacute stages of the disease. In adolescent mice, CCL11 enhances microglial cell, B and T lymphocyte migration towards the brain, whereas only the number of B lymphocytes is increased in the adult brain. Finally, the CCL11 inhibitor SB297006 significantly reversed the aforementioned effects. Our study, for the first time, demonstrates CCL11 to be a key player in mediating secondary cell injury under stroke conditions. Interfering with this pathway, as shown for SB297006, might thus be an interesting approach for future stroke treatment paradigms.
The Role of Cyclic AMP Signaling in Cardiac Fibrosis
Authors: Delaunay Marion , Osman Halima , Kaiser Simon , Diviani Dario
Published: December 2019

Myocardial stress and injury invariably promote remodeling of the cardiac tissue, which is associated with cardiomyocyte death and development of fibrosis. The fibrotic process is initially triggered by the differentiation of resident cardiac fibroblasts into myofibroblasts. These activated fibroblasts display increased proliferative capacity and secrete large amounts of extracellular matrix. Uncontrolled myofibroblast activation can thus promote heart stiffness, cardiac dysfunction, arrhythmias, and progression to heart failure. Despite the well-established role of myofibroblasts in mediating cardiac disease, our current knowledge on how signaling pathways promoting fibrosis are regulated and coordinated in this cell type is largely incomplete. In this respect, cyclic adenosine monophosphate (cAMP) signaling acts as a major modulator of fibrotic responses activated in fibroblasts of injured or stressed hearts. In particular, accumulating evidence now suggests that upstream cAMP modulators including G protein-coupled receptors, adenylyl cyclases (ACs), and phosphodiesterases (PDEs); downstream cAMP effectors such as protein kinase A (PKA) and the guanine nucleotide exchange factor Epac; and cAMP signaling organizers such as A-kinase anchoring proteins (AKAPs) modulate a variety of fundamental cellular processes involved in myocardial fibrosis including myofibroblast differentiation, proliferation, collagen secretion, and invasiveness. The current review will discuss recent advances highlighting the role of cAMP and AKAP-mediated signaling in regulating pathophysiological responses controlling cardiac fibrosis.
Autophagy and Macrophage Functions: Inflammatory Response and Phagocytosis
Authors: Wu Ming-Yue , Lu Jia-Hong
Published: December 2019

Autophagy is a conserved bulk degradation and recycling process that plays important roles in multiple biological functions, including inflammatory responses. As an important component of the innate immune system, macrophages are involved in defending cells from invading pathogens, clearing cellular debris, and regulating inflammatory responses. During the past two decades, accumulated evidence has revealed the intrinsic connection between autophagy and macrophage function. This review focuses on the role of autophagy, both as nonselective and selective forms, in the regulation of the inflammatory and phagocytotic functions of macrophages. Specifically, the roles of autophagy in pattern recognition, cytokine release, inflammasome activation, macrophage polarization, LC3-associated phagocytosis, and xenophagy are comprehensively reviewed. The roles of autophagy receptors in the macrophage function regulation are also summarized. Finally, the obstacles and remaining questions regarding the molecular regulation mechanisms, disease association, and therapeutic applications are discussed.
Anti-Cytokine Autoantibodies in Systemic Lupus Erythematosus
Authors: Howe Hwee Siew , Leung Bernard Pui Lam
Published: December 2019

Cytokine dysregulation is characteristic of systemic lupus erythematosus (SLE), a systemic autoimmune disease of considerable heterogeneity. Insights gained about the cytokine dysregulation in SLE have the potential for identifying patient subsets before the onset of clinical disease and during established disease. Clustering patients by cytokine and disease activity subsets is more informative than isolated cytokine studies, as both pro inflammatory and immunoregulatory cytokines contribute to the cytokine dysregulated state in SLE. Endogenous anti-cytokine autoantibodies (ACAAs) may be involved in the regulation of cytokine biology by reducing excessive production or by prolonging their half-life in the circulation through the formation of cytokine-antibody immune complexes. Although endogenous ACAAs may have deleterious effects such as contributing to immunodeficiency states, their role in the pathophysiology of autoimmune conditions such as SLE has yet to be clearly elucidated. The aim of the present article is to provide a focused review of the current knowledge of ACAAs in SLE.
Structure–Function Relationship and Physiological Roles of Transient Receptor Potential Canonical (TRPC) 4 and 5 Channels
Authors: Kim Jinsung , Ko Juyeon , Hong Chansik , So Insuk
Published: December 2019

The study of the structure–function relationship of ion channels has been one of the most challenging goals in contemporary physiology. Revelation of the three-dimensional (3D) structure of ion channels has facilitated our understanding of many of the submolecular mechanisms inside ion channels, such as selective permeability, voltage dependency, agonist binding, and inter-subunit multimerization. Identifying the structure–function relationship of the ion channels is clinically important as well since only such knowledge can imbue potential therapeutics with practical possibilities. In a sense, recent advances in the understanding of the structure–relationship of transient receptor potential canonical (TRPC) channels look promising since human TRPC channels are calcium-permeable, non-selective cation channels expressed in many tissues such as the gastrointestinal (GI) tract, kidney, heart, vasculature, and brain. TRPC channels are known to regulate GI contractility and motility, pulmonary hypertension, right ventricular hypertrophy, podocyte injury, seizure, fear, anxiety-like behavior, and many others. In this article, we tried to elaborate recent findings of Cryo-EM (cryogenic-electron microscopy) based structural information of TRPC 4 and 5 channels and domain-specific functions of the channel, such as G-protein mediated activation mechanism, extracellular modification of the channel, homo/hetero-tetramerization, and pharmacological gating mechanisms.