bluuebunny/crossref_metadata_2025 huggingface dataset:
Explaining Factors Leading to Community Acceptance of Wind Energy. Results of an Expert Assessment
Authors: De Luca Elena , Nardi Cecilia , Giuffrida Laura Gaetana , Krug Michael , Di Nucci Maria Rosaria
Published: April 2020

The present article deals with two key drivers of social acceptance of wind energy: procedural justice and distributional justice. It is based on a comparative expert assessment carried out in the frame of the Horizon 2020 project WinWind covering six European countries. The focus of the paper is on procedural and financial participation of citizens and local stakeholders in wind energy projects. The first part covers institutional arrangements for public engagement in two areas of the decision-making process—wind turbine zoning/siting in spatial plans and authorization procedures. Here, three levels of public involvement—information, consultation and participation—were analyzed. The second part examines active and financial participation of citizens and local stakeholders. Here, we distinguish between two different modes of governance: institutionalized forms of public governance and voluntary forms of corporate governance. The outcomes suggest that concrete paths to the social acceptance of wind energy are fostered via appropriate institutional spaces for public engagement. Furthermore, missing opportunities for active and passive financial participation can have strong negative consequences for community acceptance
Transcriptomic and Physiological Responses to Oxidative Stress in a Chlamydomonas reinhardtii Glutathione Peroxidase Mutant
Authors: Ma Xiaocui , Zhang Baolong , Miao Rongli , Deng Xuan , Duan You , Cheng Yingyin , Zhang Wanting , Shi Mijuan , Huang Kaiyao , Xia Xiao-Qin
Published: April 2020

Aerobic photosynthetic organisms such as algae produce reactive oxygen species (ROS) as by-products of metabolism. ROS damage biomolecules such as proteins and lipids in cells, but also act as signaling molecules. The mechanisms that maintain the metabolic balance in aerobic photosynthetic organisms and how the cells specifically respond to different levels of ROS are unclear. Glutathione peroxidase (GPX) enzymes detoxify hydrogen peroxide or organic hydroperoxides, and thus are important components of the antioxidant system. In this study, we employed a Chlamydomonas reinhardtii glutathione peroxidase knockout (gpx5) mutant to identify the genetic response to singlet oxygen (1O2) generated by the photosensitizer rose bengal (RB). To this end, we compared the transcriptomes of the parental strain CC4348 and the gpx5 mutant sampled before, and 1 h after, the addition of RB. Functional annotation of differentially expressed genes showed that genes encoding proteins related to ROS detoxification, stress-response-related molecular chaperones, and ubiquitin–proteasome pathway genes were upregulated in CC4338. When GPX5 was mutated, higher oxidative stress specifically induced the TCA cycle and enhanced mitochondrial electron transport. Transcription of selenoproteins and flagellar-associated proteins was depressed in CC4348 and the gpx5 mutant. In addition, we found iron homeostasis played an important role in maintaining redox homeostasis, and we uncovered the relationship between 1O2 stress and iron assimilation, as well as selenoproteins. Based on the observed expression profiles in response to different levels of oxidative stress, we propose a model for dose-dependent responses to different ROS levels in Chlamydomonas.
Multi-Attribute Analysis Using Coherency and Ant-Tracking Techniques for Fault and Fracture Detection in La Florida Anticline, Llanos Foothills, Colombia
Authors: Albesher Ziyad , Kellogg James , Hafiza Ibraheem , Saeid Essam
Published: April 2020

We present techniques to reduce noise and enhance seismic quality, making possible the first multi-attribute analysis of a 3D seismic volume in the Llanos Foothills (La Florida anticline) of Colombia using coherency and ant-tracking techniques for fault and fracture detection. The results could help reduce risk in models of reservoir fracture porosity and permeability. The dominant fracture strike direction in the studied seismic volume (La Florida anticline) is NE–SW (055 ± 20°), parallel to the structural strike of the adjacent Eastern Cordillera Foothills. The application of the ant-tracking technique also reveals the NE-SW fracture set for the reservoir rocks in the La Florida anticline as well as in the non-folded reservoir rocks to the SE. We compared the fracture intensity and orientation in folded rocks with the fracture intensity and orientation in non-folded rocks. Our study showed NE-SW, NW-SE, and E-W fracture orientations in the non-folded seismic volume, suggesting that regional stresses could produce these fracture sets, not just folding processes as previously proposed. The NW-SE and WNW-ESE fracture sets are only found in the Guayabo Formation (11 Ma–Present). A right–lateral strike–slip displacement on the nearby Algeciras fault system in the last 2 m.y. may have generated WNW-ESE and NW-SE Riedel-type shear fractures in the study area.
Psychological Resilience of Volunteers in a South African Health Care Context: A Salutogenic Approach and Hermeneutic Phenomenological Inquiry
Authors: Barnard Antoni , Furtak Aleksandra
Published: April 2020

Volunteering in non-Western countries, such as South Africa, is subject to poor infrastructure, lack of resources, poverty-stricken conditions and often conducted by volunteers from lower socio-economic spheres of society. Sustaining the well-being of volunteers in this context is essential in ensuring their continued capacity to volunteer. To do so, it is important to understand the psychological resilience of these volunteers and the resistance resources they employ to positively adapt to their challenging work-life circumstances. The aim of this qualitative hermeneutic phenomenological study was to explore volunteers’ psychological resilience from a salutogenic perspective. In-depth interviews were conducted with eight volunteers servicing government-run hospitals. Data were analysed through phenomenological hermeneutical analysis. Findings show a characteristic work-life orientation to be at the root of volunteers’ resilience. Their work-life orientation is based on a distinct inner drive, an other-directedness and a “calling” work orientation. It is proposed that this work-life orientation enables volunteers in this study context, to cope with and positively adapt to challenging work-life circumstances and continue volunteering. The elements of their work-life orientation are presented as intrapersonal strength resources fundamental to their psychological resilience. It is suggested that organisations invest in developmental interventions that endorse and promote these intrapersonal strengths.
The Risk of Venous Thromboembolism in Patients with Gallstones
Authors: Chen Chien-Hua , Lin Cheng-Li , Kao Chia-Hung
Published: April 2020

The objective of this study is to assess the relationship between gallstones and venous thromboembolism (VTE), including deep vein thrombosis (DVT) and pulmonary embolism (PE), and the risk of VTE after cholecystectomy for gallstones. This nationwide population-based cohort study retrieved the hospitalization database from the Longitudinal Health Insurance Research Database (LHID2000), a database belonging to the National Health Insurance (NHI) program of Taiwan. A total of 345,793 patients aged ≥ 18 years with gallstones diagnosed between 2000 and 2010 were identified as the study cohort. The beneficiaries without gallstones were randomly selected as the control cohort by propensity score matching with the study cohort at a 1:1 ratio based on age, sex, urbanization, occupation, comorbidities, and year of the index date. We compared the risk of VTE between both cohorts and measured the risk differences of VTE between the gallstones patients with (n = 194,187) and without cholecystectomy (n = 151,606). Each patient was examined from the index date until the occurrence of DVT or PE, death or withdrawal from the NHI program, or the end of 2011. The incidence rate of DVT was 7.94/10,000 person-years for the non-gallstones cohort and 9.64/10,000 person-years for the gallstones cohort (hazard ratio (HR) = 1.35, 95% confidence interval (CI) = 1.25–1.47), respectively (p < 0.001). The incidence rate of PE was 3.92/10,000 person-years for the non-gallstones cohort and 4.65/10,000 person-years for the gallstones cohort (HR = 1.35, 95% CI = 1.20–1.53), respectively (p < 0.001). The cumulative incidence of DVT (6.54/10,000 person-years vs 14.6/10,000 person-years, adjusted hazard ratio (aHR) = 0.60, 95% CI = 0.54–0.67) and PE (3.29/10,000 person-years vs 6.84/10,000 person-years, aHR = 0.67, 95% CI = 0.58–0.77) for gallstones patients was lower in the cholecystectomy cohort than that in the non-cholecystectomy cohort after adjustment for age, sex, urbanization level, occupation, frequency of medical visits, history of pregnancy, and comorbidities (log-rank test, p < 0.001). Our findings indicate that the risk of DVT or PE in patients with gallstones was greater than those without gallstones. However, the risk of DVT and PE in the patients with gallstones would decrease after cholecystectomy. This area of research needs more studies to ascertain the pathogenesis for the contribution of gallstones to the development of VTE and the protective mechanisms of cholecystectomy against the development of VTE.
Impact of Formal Care Use on Informal Care from Children after the Launch of Long-Term Care Insurance in Shanghai, China
Authors: Zhang Huimin , Zhang Xiaoyi , Zhao Youhua , Huang Jianfeng , Liu Wenwei
Published: April 2020

The impact of formal care (co-paid by long term care (LTC) insurance) on informal care is critical to the improvement and promotion of public policy. We conducted an interview-based survey to examine how the use of formal care impacts the use of informal care in Shanghai, which was one of China’s first long-term insurance pilots in 2016. In addition to total informal care time, the following four types of informal care were considered: (1) household activities of daily living (HDL) tasks, (2) activities of daily living (ADL) tasks, (3) instrumental activities of daily living (IADL) tasks, and (4) supervision tasks. Of the 407 families, an average of 12.36 h (SD = 6.70) of informal care was crowded out each week. Among them, ADL tasks, HDL tasks, and supervision tasks were reduced an average of 4.60 (SD = 3.59), 5.50 (SD = 3.38), and 2.10 h (SD = 3.06) per week, respectively. Each additional hour of formal care reduced 0.473 h of informal care. Care recipients’ gender and health status were also determined to be associated with crowding out hours of informal care. These findings can be utilized as empirical evidence for decision-makers to consider the scope of funding for formal care, and this study provides comparable results to developing countries and regions.
Analysis of Job-Related Demands and Resources in Ambulatory Youth Welfare Services: A Qualitative and Quantitative Approach
Authors: Vincent-Höper Sylvie , Lengen Julia C. , Kersten Maren , Gregersen Sabine
Published: April 2020

In this study, we investigated health-relevant job characteristics of social workers in ambulatory youth welfare services, combining qualitative and quantitative methods. Based on a systematic literature review, expert workshops, and focus group discussions with 9 experts of the target group, we identified target group-specific job demands and job resources, which we compiled into a questionnaire using content-valid scales. The target group-specific survey tool comprises 9 scales for assessing job demands and 10 scales for assessing job resources. Analyses of data from 209 social workers demonstrated desirable psychometric properties and substantial correlations of the scales with coping behaviours and indicators of employee well-being. The scales for assessing job demands were negatively related to psychological well-being and job satisfaction and positively related to burnout and depressiveness. The scales for assessing job resources showed positive correlations with indicators of positive well-being and negative correlations with indicators of impaired well-being. Regression analyses revealed that job resources explained a higher amount of variance in the positive well-being indicators compared to job demands. The study identified a broad range of health-relevant job characteristics for social workers in ambulatory youth welfare. Applying the target group-specific survey tool allows organisations to derive suitable implications for the design of health promotion programs.
Workplace Mobbing in Polish and Lithuanian Organisations with Regard to Corporate Social Responsibility
Authors: Vveinhardt Jolita , Sroka Włodzimierz
Published: April 2020

The ‘mobbing’ phenomenon is regarded as the actions or behaviour referring to an employee or directed against an employee, comprising persistent and drawn-out harassment or intimidation of that employee. This phenomenon causes substantial negative workplace consequences, but, above all, one should stress the consequences for the victims, which are devastating. This has been observed in a variety of organisations, regardless of the sector and country. Given these facts, the purpose of this study was to identify the prevalence of workplace mobbing in Polish and Lithuanian organisations with regard to corporate social responsibility (CSR). The research sample included a group of 823 entities operating in both countries in both the private and public sectors (410 from Lithuania and 413 from Poland). A closed-type questionnaire was used in the survey. Several research methods including factor analysis, Cronbach’s alpha, Spearman–Brown, factor loading, and total item correlation were used in our study. The results achieved showed that there were both similarities as well as differences between the analysed organisations. More specifically, our research revealed that: (1) Employee attitude to CSR depends on the company’s sector of activity and the country; (2) In Poland, workplace mobbing is more prevalent in the public sector than in the private, whilst in Lithuania there were no substantial differences; (3) Organisations that implemented the CSR concept showed less imposed mobbing prevalence; and (4) Employees who faced mobbing in the workplace had worse relationships with clients and users of the company’s services/products.
The Effects of Pharmacological and Non-Pharmacological Interventions on Symptom Management and Quality of Life among Breast Cancer Survivors Undergoing Adjuvant Endocrine Therapy: A Systematic Review
Authors: Chan Carmen W.H. , Tai Daria , Kwong Stephanie , Chow Ka Ming , Chan Dorothy N.S. , Law Bernard M.H.
Published: April 2020

Breast cancer survivors need to undergo adjuvant endocrine therapy after completion of curative treatments to prevent disease recurrence. These individuals often experience symptoms which are detrimental to their quality of life (QOL). Implementation of interventions for effective symptom management among these survivors is warranted. This review provides an overview of studies on the effectiveness of the previously developed interventions for breast cancer survivors undergoing adjuvant endocrine therapy on symptom alleviation and enhancement of QOL or health-related QOL (HRQOL). Five electronic databases were employed in the literature search. Study selection, data extraction and critical appraisal of the included studies were conducted by three authors independently. Twenty-four studies were included. Both pharmacological and non-pharmacological interventions are effective in addressing the symptoms associated with adjuvant endocrine therapy among the breast cancer survivors, and in improving their QOL, although discrepancies were noted between the studies in terms of the significance of these effects. Pharmacological and non-pharmacological interventions can be effective for symptom management among breast cancer survivors. Their implementation is recommended for effective survivorship care for these individuals. Further research on intervention development for breast cancer survivors is recommended to provide further evidence for the utility of the explored interventions in survivorship care for these patients.
A New Feature with the Potential to Detect the Severity of Obstructive Sleep Apnoea via Snoring Sound Analysis
Authors: Hayashi Shota , Tamaoka Meiyo , Tateishi Tomoya , Murota Yuki , Handa Ibuki , Miyazaki Yasunari
Published: April 2020

The severity of obstructive sleep apnoea (OSA) is diagnosed with polysomnography (PSG), during which patients are monitored by over 20 physiological sensors overnight. These sensors often bother patients and may affect patients’ sleep and OSA. This study aimed to investigate a method for analyzing patient snore sounds to detect the severity of OSA. Using a microphone placed at the patient’s bedside, the snoring and breathing sounds of 22 participants were recorded while they simultaneously underwent PSG. We examined some features from the snoring and breathing sounds and examined the correlation between these features and the snore-specific apnoea-hypopnea index (ssAHI), defined as the number of apnoea and hypopnea events during the hour before a snore episode. Statistical analyses revealed that the ssAHI was positively correlated with the Mel frequency cepstral coefficients (MFCC) and volume information (VI). Based on clustering results, mild snore sound episodes and snore sound episodes from mild OSA patients were mainly classified into cluster 1. The results of clustering severe snore sound episodes and snore sound episodes from severe OSA patients were mainly classified into cluster 2. The features of snoring sounds that we identified have the potential to detect the severity of OSA.
Musculoskeletal Disorders Symptoms among Taiwanese Bakery Workers
Authors: Chen Yi-Lang , Zhong Yan-Ting , Liou Bang-Nan , Yang Chih-Chuan
Published: April 2020

In this study, the Nordic Musculoskeletal Questionnaire (NMQ) was administered to a valid sample of 81 Taiwanese bakery workers to explore their discomfort or symptoms of work-related musculoskeletal disorders and identify the risk factors. Wrist postures were also examined during 3 typical dough operations (kneading, rolling, and rounding) by using an electrogoniometer. The prevalence of musculoskeletal discomfort in any part of the body in the past year among the respondents was 93.0%, with the highest prevalence of 66.3% and 51.8% in the hands/wrists (right and left), followed by the prevalence of 50.6% and 45.8% in the shoulders (right and left) and the lower back (48.2%), respectively. The results also revealed that during the 3 dough processing operations, the workers’ wrist movements in specific operations were close to the recommended limits suggested in previous studies, especially the ulnar deviation and palm flexion of the right wrist during dough kneading and the radial deviation of the left wrist during dough rolling and rounding. The study findings can be used to explain why the bakers self-report a high proportion of wrist and shoulder disorders and can also serve as a reference for task rearrangement and redesign.
Assessments of Water-Soluble Inorganic Ions and Heavy Metals in Atmospheric Dustfall and Topsoil in Lanzhou, China
Authors: Li Yingquan , Zhao Baowei , Duan Kaixiang , Cai Juexian , Niu Wujiang , Dong Xiao
Published: April 2020

The chemical features of atmospheric dustfall and topsoil in the same region could reflect the processes of the migration, transport, and diffusion of pollutants in the atmospheric-soil system. Samples of atmospheric dustfall and topsoil were collected in Lanzhou City. The contents and correlation of water-soluble inorganic ions (WSIIs) and heavy metals in dustfall and topsoil were analyzed, the sources of heavy metals and WSIIs in dustfall were distinguished, and the potential ecological risks of heavy metals in dustfall and topsoil were evaluated. The highest contents of WSIIs are SO42− (18,594 mg·kg−1) and Ca2+ (10,070 mg·kg−1) in dustfall, and for SO42− (8271 mg·kg−1) and Na+ (1994 mg·kg−1) in topsoil. The concentrations of heavy metals (Pb, Cu, Zn, Cr, Cd, and Ni) in dustfall are considerably higher than those in topsoil. Combustion of biomass and coal, transportation and industrial activities are the major anthropogenic sources of WSIIs and heavy metals in Lanzhou. Pollution of heavy metals except Cr and Ni in dustfall, and Cu, Cr, and Ni in topsoil was up to different degrees, where the pollution of Cd was serious. The risk of Cd in dustfall is high while moderate in topsoil. This research could offer a reference for the atmospheric particle pollution prevention and control in Lanzhou.
Do Aging and Low Fertility Reduce Carbon Emissions in Korea? Evidence from IPAT Augmented EKC Analysis
Authors: Kim Jaehyeok , Lim Hyungwoo , Jo Ha-Hyun
Published: April 2020

The purpose of this article is to empirically find the Environmental Kuznets Curve (EKC) relationship between income and carbon dioxide (CO2) emissions and to analyze the influence of population aging on such emissions. We utilize Korean regional panel data of 16 provinces during the period from 1998 to 2016. To account for the nonstationary time series in the panel, we employ a fully modified ordinary least squares (FMOLS) and estimate long-run elasticity. From the empirical results, we can find the nonlinear relationship between income and CO2 emissions. Additionally, we verify the fact that population aging reduces CO2 emissions. A 1% increase in the proportion of the elderly results in a 0.4% decrease in CO2 emissions. On the other hand, the younger population increases CO2 emissions. These results were in line with those of additional analysis on residential and transportation CO2 emissions, for the robustness check.
Impact of the Dam Construction on the Downstream Thermal Conditions of the Yangtze River
Authors: He Tianfu , Deng Yun , Tuo Youcai , Yang Yanjing , Liang Naisheng
Published: April 2020

Water temperature is an important factor in aquatic environments. Dam construction, especially the construction of multiple dams in rivers, can greatly affect the downstream water temperature. Several dams, including Wudongde, Baihetan, Xiluodu, Xiangjiaba, Three Gorges, and Gezhouba, have been constructed between Panzhihua and Yichang along the Yangtze River. The aim of this paper was to quantify the impact of these dams on the water temperature downstream. One-dimensional and two-dimensional models were used to simulate the water temperatures, and the results showed that the dams had different cumulative effects on it. For example, in January, after the construction of the Xiangjiaba and Xiluodu dams, the discharge water temperature of Xiangjiaba was 3 °C higher than the natural conditions, and after the construction of the Baihetan and Wudongde dams was completed, it increased by a further 2 °C. The natural river ran over 416 km with no dams from the Xiangjiaba dam to the Cuntan Station. With the influence of climate and tributary inflow, the impact of upstream dams on the water temperature was mitigated by more than 48% at Cuntan Station, displaying a recovery. It seemed that the cumulative effects of dams on the discharge water temperature of the Three Gorges decreased with the increase in the upstream storage capacity from March to May, and the construction of dams even had a negative effect. From September to February of the next year, the cumulative effects increased with the increase of the upstream storage capacity, but only the total storage capacity until a certain level, where no further impact was observed.
Scapular Dyskinesis: From Basic Science to Ultimate Treatment
Authors: Giuseppe Longo Umile , Laura Risi Ambrogioni , Berton Alessandra , Candela Vincenzo , Massaroni Carlo , Carnevale Arianna , Stelitano Giovanna , Schena Emiliano , Nazarian Ara , DeAngelis Joseph , Denaro Vincenzo
Published: April 2020

Background: This study intends to summarize the causes, clinical examination, and treatments of scapular dyskinesis (SD) and to briefly investigate whether alteration can be managed by a precision rehabilitation protocol planned on the basis of features derived from clinical tests. Methods: We performed a comprehensive search of PubMed, Cochrane, CINAHL and EMBASE databases using various combinations of the keywords “Rotator cuff”, “Scapula”, “Scapular Dyskinesis”, “Shoulder”, “Biomechanics” and “Arthroscopy”. Results: SD incidence is growing in patients with shoulder pathologies, even if it is not a specific injury or directly related to a particular injury. SD can be caused by multiple factors or can be the trigger of shoulder-degenerative pathologies. In both cases, SD results in a protracted scapula with the arm at rest or in motion. Conclusions: A clinical evaluation of altered shoulder kinematics is still complicated. Limitations in observing scapular motion are mainly related to the anatomical position and function of the scapula itself and the absence of a tool for quantitative SD clinical assessment. High-quality clinical trials are needed to establish whether there is a possible correlation between SD patterns and the specific findings of shoulder pathologies with altered scapular kinematics.
HIF-1β Positively Regulates NF-κB Activity via Direct Control of TRAF6
Authors: D’Ignazio Laura , Shakir Dilem , Batie Michael , Muller H. Arno , Rocha Sonia
Published: April 2020

NF-κB signalling is crucial for cellular responses to inflammation but is also associated with the hypoxia response. NF-κB and hypoxia inducible factor (HIF) transcription factors possess an intense molecular crosstalk. Although it is known that HIF-1α modulates NF-κB transcriptional response, very little is understood regarding how HIF-1β contributes to NF-κB signalling. Here, we demonstrate that HIF-1β is required for full NF-κB activation in cells following canonical and non-canonical stimuli. We found that HIF-1β specifically controls TRAF6 expression in human cells but also in Drosophila melanogaster. HIF-1β binds to the TRAF6 gene and controls its expression independently of HIF-1α. Furthermore, exogenous TRAF6 expression is able to rescue all of the cellular phenotypes observed in the absence of HIF-1β. These results indicate that HIF-1β is an important regulator of NF-κB with consequences for homeostasis and human disease.
Deubiquitinase MYSM1 in the Hematopoietic System and beyond: A Current Review
Authors: Fiore Amanda , Liang Yue , Lin Yun Hsiao , Tung Jacky , Wang HanChen , Langlais David , Nijnik Anastasia
Published: April 2020

MYSM1 has emerged as an important regulator of hematopoietic stem cell function, blood cell production, immune response, and other aspects of mammalian physiology. It is a metalloprotease family protein with deubiquitinase catalytic activity, as well as SANT and SWIRM domains. MYSM1 normally localizes to the nucleus, where it can interact with chromatin and regulate gene expression, through deubiquitination of histone H2A and non-catalytic contacts with other transcriptional regulators. A cytosolic form of MYSM1 protein was also recently described and demonstrated to regulate signal transduction pathways of innate immunity, by promoting the deubiquitination of TRAF3, TRAF6, and RIP2. In this work we review the current knowledge on the molecular mechanisms of action of MYSM1 protein in transcriptional regulation, signal transduction, and potentially other cellular processes. The functions of MYSM1 in different cell types and aspects of mammalian physiology are also reviewed, highlighting the key checkpoints in hematopoiesis, immunity, and beyond regulated by MYSM1. Importantly, mutations in MYSM1 in human were recently linked to a rare hereditary disorder characterized by leukopenia, anemia, and other hematopoietic and developmental abnormalities. Our growing knowledge of MYSM1 functions and mechanisms of actions sheds important insights into its role in mammalian physiology and the etiology of the MYSM1-deficiency disorder in human.
Fear Learning Enhances Prefrontal Cortical Suppression of Auditory Thalamic Inputs to the Amygdala in Adults, but Not Adolescents
Authors: Ferrara Nicole C. , Mrackova Eliska , Loh Maxine K. , Padival Mallika , Rosenkranz J. Amiel
Published: April 2020

Adolescence is characterized by increased susceptibility to the development of fear- and anxiety-related disorders. Adolescents also show elevated fear responding and aversive learning that is resistant to behavioral interventions, which may be related to alterations in the circuitry supporting fear learning. These features are linked to ongoing adolescent development of medial prefrontal cortical (PFC) inputs to the basolateral amygdala (BLA) that regulate neural activity and contribute to the refinement of fear responses. Here, we tested the hypothesis that the extent of PFC inhibition of the BLA following fear learning is greater in adults than in adolescents, using anesthetized in vivo recordings to measure local field potentials (LFPs) evoked by stimulation of PFC or auditory thalamic (MgN) inputs to BLA. We found that BLA LFPs evoked by stimulation of MgN inputs were enhanced in adults following fear conditioning. Fear conditioning also led to reduced summation of BLA LFPs evoked in response to PFC train stimulation, and increased the capacity of PFC inhibition of MgN inputs in adults. These data suggest that fear conditioning recruits additional inhibitory capacity by PFC inputs to BLA in adults, but that this capacity is weaker in adolescents. These results provide insight into how the development of PFC inputs may relate to age differences in memory retention and persistence following aversive learning.
Novel Insights into the Pathogenesis of Spinal Sarcopenia and Related Therapeutic Approaches: A Narrative Review
Authors: Kuo Yu-Kai , Lin Yu-Ching , Lee Ching-Yu , Chen Chih-Yu , Tani Jowy , Huang Tsung-Jen , Chang Hsi , Wu Meng-Huang
Published: April 2020

Spinal sarcopenia is a complex and multifactorial disorder associated with a loss of strength, increased frailty, and increased risks of fractures and falls. In addition, spinal sarcopenia has been associated with lumbar spine disorders and osteoporosis, which renders making decisions on treatment modalities difficult. Patients with spinal sarcopenia typically exhibit lower cumulative survival, a higher risk of in-hospital complications, prolonged hospital stays, higher postoperative costs, and higher rates of blood transfusion after thoracolumbar spine surgery. Several studies have focused on the relationships between spinal sarcopenia, appendicular muscle mass, and bone-related problems—such as osteoporotic fractures and low bone mineral density—and malnutrition and vitamin D deficiency. Although several techniques are available for measuring sarcopenia, each of them has its advantages and shortcomings. For treating spinal sarcopenia, nutrition, physical therapy, and medication have been proven to be effective; regenerative therapeutic options seem to be promising owing to their repair and regeneration potential. Therefore, in this narrative review, we summarize the characteristics, detection methodologies, and treatment options for spinal sarcopenia, as well as its role in spinal disorders.
Role of Non-Coding RNAs in Lung Circadian Clock Related Diseases
Authors: Chinnapaiyan Srinivasan , Dutta Rajib Kumar , Devadoss Dinesh , Chand Hitendra S , Rahman Irfan , Unwalla Hoshang Jehangir
Published: April 2020

Circadian oscillations are regulated at both central and peripheral levels to maintain physiological homeostasis. The central circadian clock consists of a central pacemaker in the suprachiasmatic nucleus that is entrained by light dark cycles and this, in turn, synchronizes the peripheral clock inherent in other organs. Circadian dysregulation has been attributed to dysregulation of peripheral clock and also associated with several diseases. Components of the molecular clock are disrupted in lung diseases like chronic obstructive pulmonary disease (COPD), asthma and IPF. Airway epithelial cells play an important role in temporally organizing magnitude of immune response, DNA damage response and acute airway inflammation. Non-coding RNAs play an important role in regulation of molecular clock and in turn are also regulated by clock components. Dysregulation of these non-coding RNAs have been shown to impact the expression of core clock genes as well as clock output genes in many organs. However, no studies have currently looked at the potential impact of these non-coding RNAs on lung molecular clock. This review focuses on the ways how these non-coding RNAs regulate and in turn are regulated by the lung molecular clock and its potential impact on lung diseases.
The Role of miRNA and Related Pathways in Pathophysiology of Uterine Fibroids—From Bench to Bedside
Authors: Ciebiera Michał , Włodarczyk Marta , Zgliczyński Stanisław , Łoziński Tomasz , Walczak Klaudia , Czekierdowski Artur
Published: April 2020

Uterine fibroids (UFs) are the most common benign tumors of the female genital tract. Their prevalence usually is estimated at 30–40%, but may reach up to 70–80% in predisposed groups of women. UFs may cause various clinical issues which might constitute the major reason of the overall deterioration of the quality of life. The mechanisms leading to UFs formation and growth still remain poorly understood. The transformation of smooth muscle cells of the uterus into abnormal, immortal cells, capable of clonal division, is thought to be a starting point of all pathways leading to UF formation. Micro-ribonucleic acids (miRNAs) are non-coding single-stranded RNAs about 22 nucleotides in length, that regulate gene expression. One of recent advances in this field is the comprehension of the role of miRNAs in tumorigenesis. Alterations in the levels of miRNAs are related to the formation and growth of several tumors which show a distinct miRNA signature. The aim of this review is to summarize the current data about the role of miRNAs in the pathophysiology of UFs. We also discuss future directions in the miRNA research area with an emphasis on novel diagnostic opportunities or patient-tailored therapies. In our opinion data concerning the regulation of miRNA and its gene targets in the UFs are still insufficient in comparison with gynecological malignancies. The potential translational use of miRNA and derived technologies in the clinical care is at the early phase and needs far more evidence. However, it is one of the main areas of interest for the future as the use of miRNAs in the diagnostics and treatment of UFs is a new and exciting opportunity.
Sweet Cherry (Prunus avium L.) PaPIP1;4 Is a Functional Aquaporin Upregulated by Pre-Harvest Calcium Treatments that Prevent Cracking
Authors: Breia Richard , Mósca Andreia F. , Conde Artur , Correia Sofia , Conde Carlos , Noronha Henrique , Soveral Graça , Gonçalves Berta , Gerós Hernâni
Published: April 2020

The involvement of aquaporins in rain-induced sweet cherry (Prunus avium L.) fruit cracking is an important research topic with potential agricultural applications. In the present study, we performed the functional characterization of PaPIP1;4, the most expressed aquaporin in sweet cherry fruit. Field experiments focused on the pre-harvest exogenous application to sweet cherry trees, cultivar Skeena, with a solution of 0.5% CaCl2, which is the most common treatment to prevent cracking. Results show that PaPIP1;4 was mostly expressed in the fruit peduncle, but its steady-state transcript levels were higher in fruits from CaCl2-treated plants than in controls. The transient expression of PaPIP1;4-GFP in tobacco epidermal cells and the overexpression of PaPIP1;4 in YSH1172 yeast mutation showed that PaPIP1;4 is a plasma membrane protein able to transport water and hydrogen peroxide. In this study, we characterized for the first time a plasma membrane sweet cherry aquaporin able to transport water and H2O2 that is upregulated by the pre-harvest exogenous application of CaCl2 supplements.
Wood Architecture and Composition Are Deeply Remodeled in Frost Sensitive Eucalyptus Overexpressing CBF/DREB1 Transcription Factors
Authors: Cao Phi Bang , Ployet Raphaël , Nguyen Chien , Dupas Annabelle , Ladouce Nathalie , Martinez Yves , Grima-Pettenati Jacqueline , Marque Christiane , Mounet Fabien , Teulières Chantal
Published: April 2020

Eucalypts are the most planted trees worldwide, but most of them are frost sensitive. Overexpressing transcription factors for CRT-repeat binding factors (CBFs) in transgenic Eucalyptus confer cold resistance both in leaves and stems. While wood plays crucial roles in trees and is affected by environmental cues, its potential role in adaptation to cold stress has been neglected. Here, we addressed this question by investigating the changes occurring in wood in response to the overexpression of two CBFs, taking advantage of available transgenic Eucalyptus lines. We performed histological, biochemical, and transcriptomic analyses on xylem samples. CBF ectopic expression led to a reduction of both primary and secondary growth, and triggered changes in xylem architecture with smaller and more frequent vessels and fibers exhibiting reduced lumens. In addition, lignin content and syringyl/guaiacyl (S/G) ratio increased. Consistently, many genes of the phenylpropanoid and lignin branch pathway were upregulated. Most of the features of xylem remodeling induced by CBF overexpression are reminiscent of those observed after long exposure of Eucalyptus trees to chilling temperatures. Altogether, these results suggest that CBF plays a central role in the cross-talk between response to cold and wood formation and that the remodeling of wood is part of the adaptive strategies to face cold stress.
Increased Protein S-Glutathionylation in Leber’s Hereditary Optic Neuropathy (LHON)
Authors: Zhou Lei , Chan James Chun Yip , Chupin Stephanie , Gueguen Naïg , Desquiret-Dumas Valérie , Koh Siew Kwan , Li Jianguo , Gao Yan , Deng Lu , Verma Chandra , Beuerman Roger W , Chan Eric Chun Yong , Milea Dan , Reynier Pascal
Published: April 2020

Leber’s hereditary optic neuropathy (LHON, MIM#535000) is the most common form of inherited optic neuropathies and mitochondrial DNA-related diseases. The pathogenicity of mutations in genes encoding components of mitochondrial Complex I is well established, but the underlying pathomechanisms of the disease are still unclear. Hypothesizing that oxidative stress related to Complex I deficiency may increase protein S-glutathionylation, we investigated the proteome-wide S-glutathionylation profiles in LHON (n = 11) and control (n = 7) fibroblasts, using the GluICAT platform that we recently developed. Glutathionylation was also studied in healthy fibroblasts (n = 6) after experimental Complex I inhibition. The significantly increased reactive oxygen species (ROS) production in the LHON group by Complex I was shown experimentally. Among the 540 proteins which were globally identified as glutathionylated, 79 showed a significantly increased glutathionylation (p < 0.05) in LHON and 94 in Complex I-inhibited fibroblasts. Approximately 42% (33/79) of the altered proteins were shared by the two groups, suggesting that Complex I deficiency was the main cause of increased glutathionylation. Among the 79 affected proteins in LHON fibroblasts, 23% (18/79) were involved in energetic metabolism, 31% (24/79) exhibited catalytic activity, 73% (58/79) showed various non-mitochondrial localizations, and 38% (30/79) affected the cell protein quality control. Integrated proteo-metabolomic analysis using our previous metabolomic study of LHON fibroblasts also revealed similar alterations of protein metabolism and, in particular, of aminoacyl-tRNA synthetases. S-glutathionylation is mainly known to be responsible for protein loss of function, and molecular dynamics simulations and 3D structure predictions confirmed such deleterious impacts on adenine nucleotide translocator 2 (ANT2), by weakening its affinity to ATP/ADP. Our study reveals a broad impact throughout the cell of Complex I-related LHON pathogenesis, involving a generalized protein stress response, and provides a therapeutic rationale for targeting S-glutathionylation by antioxidative strategies.
Checklist for Expert Evaluation of HMIs of Automated Vehicles—Discussions on Its Value and Adaptions of the Method within an Expert Workshop
Authors: Schömig Nadja , Wiedemann Katharina , Hergeth Sebastian , Forster Yannick , Muttart Jeffrey , Eriksson Alexander , Mitropoulos-Rundus David , Grove Kevin , Krems Josef , Keinath Andreas , Neukum Alexandra , Naujoks Frederik
Published: April 2020

Within a workshop on evaluation methods for automated vehicles (AVs) at the Driving Assessment 2019 symposium in Santa Fe; New Mexico, a heuristic evaluation methodology that aims at supporting the development of human–machine interfaces (HMIs) for AVs was presented. The goal of the workshop was to bring together members of the human factors community to discuss the method and to further promote the development of HMI guidelines and assessment methods for the design of HMIs of automated driving systems (ADSs). The workshop included hands-on experience of rented series production partially automated vehicles, the application of the heuristic assessment method using a checklist, and intensive discussions about possible revisions of the checklist and the method itself. The aim of the paper is to summarize the results of the workshop, which will be used to further improve the checklist method and make the process available to the scientific community. The participants all had previous experience in HMI design of driver assistance systems, as well as development and evaluation methods. They brought valuable ideas into the discussion with regard to the overall value of the tool against the background of the intended application, concrete improvements of the checklist (e.g., categorization of items; checklist items that are currently perceived as missing or redundant in the checklist), when in the design process the tool should be applied, and improvements for the usability of the checklist.
Comparison of Survival and Development of Gypsy Moth Lymantria dispar L. (Lepidoptera: Erebidae) Populations from Different Geographic Areas on North American Conifers
Authors: Keena Melody A. , Richards Jessica Y.
Published: April 2020

Host utilization information is critical to managers for estimating the hosts at risk and potential geographic range for gypsy moths from different geographic origins. In this study, the development and survival of gypsy moths from all three subspecies on 13 North American conifers and three broadleaf hosts were compared. There was variation in the ability of gypsy moth larvae from different geographic origins to utilize (survive and develop on) key North American conifers. However, that variation was not consistent within gypsy moth subspecies, but instead was more consistent with populations from different origins being preadapted to utilize different hosts and having different biologic traits. Some Asian populations developed and survived well on some conifers while populations from Europe and North America gained weight faster and/or survived better than some Asian populations. Although development was slower and survival poorer on several of the conifers, first instar larvae were able to utilize conifers unless the needles were tough or feeding deterrents were present. Host phenology was also critical since the early instars fed preferentially on new foliage or buds. Gypsy moth larvae can utilize many hosts, so this makes it a very adaptable invasive species that warrants taking measures to prevent its spread.
The Effect of Defects in Piezoelectric Composite Half-Planes with Surface Electrodes
Authors: Aboudi Jacob
Published: April 2020

An analysis for the prediction of the electromechanical field in composite piezoelectric half-planes with attached surface electrode is presented. The composite half-planes are composed of distinct constituents and may include internal defects in various locations. The solution is carried out in a sufficiently large rectangular region, the boundary conditions of which are obtained from the corresponding solution of a homogeneous piezoelectric half-plane. This is followed by the application of the discrete Fourier transform at the domain of which a boundary-value problem is formulated. The solution of this boundary-value problem, followed by the inversion of the Fourier transform, provides, in conjunction with an iterative procedure, the electromechanical field at any point of the rectangular region. Applications are given for a piezoelectric half-plane with defects in the form of a cavity and of short and semi-infinite cracks as well as of a periodically bilayered composite with a crack in one of its layers.
Biocompatible Gels of Chitosan–Buriti Oil for Potential Wound Healing Applications
Authors: Ferreira Maria Onaira Gonçalves , Lima Idglan Sá , Ribeiro Alessandra Braga , Lobo Anderson O. , Rizzo Marcia S. , Osajima Josy Anteveli , Estevinho Leticia Miranda , Silva-Filho Edson C.
Published: April 2020

The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications. This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent. The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis. Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated. Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified. The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL. Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals. Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction. The results indicated that the gel chitosan–buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
Dislocation Density Based Flow Stress Model Applied to the PFEM Simulation of Orthogonal Cutting Processes of Ti-6Al-4V
Authors: Rodríguez Juan Manuel , Larsson Simon , Carbonell Josep Maria , Jonsén Pär
Published: April 2020

Machining of metals is an essential operation in the manufacturing industry. Chip formation in metal cutting is associated with large plastic strains, large deformations, high strain rates and high temperatures, mainly located in the primary and in the secondary shear zones. During the last decades, there has been significant progress in numerical methods and constitutive modeling for machining operations. In this work, the Particle Finite Element Method (PFEM) together with a dislocation density (DD) constitutive model are introduced to simulate the machining of Ti-6Al-4V. The work includes a study of two constitutive models for the titanium material, the physically based plasticity DD model and the phenomenology based Johnson–Cook model. Both constitutive models were implemented into an in-house PFEM software and setup to simulate deformation behaviour of titanium Ti6Al4V during an orthogonal cutting process. Validation show that numerical and experimental results are in agreement for different cutting speeds and feeds. The dislocation density model, although it needs more thorough calibration, shows an excellent match with the results. This paper shows that the combination of PFEM together with a dislocation density constitutive model is an excellent candidate for future numerical simulations of mechanical cutting.