bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Helicobacters in Neuropsychiatric Disease: A Systematic Review in Idiopathic Parkinsonism
Authors: Tucker Rosalind M. , Augustin Aisha D. , Hayee Bu’ Hussain , Bjarnason Ingvar , Taylor David , Weller Clive , Charlett André , Dobbs Sylvia M , Dobbs R John
Published: July 2020

Interest in an aetiopathogenic role for Helicobacter in neuropsychiatric diseases started with idiopathic parkinsonism (IP), where the cardinal signs can be assessed objectively. This systematic review, using an EMBASE database search, addresses Oxford Centre for Evidence-Based Medicine based questions on the inter-relationship of Helicobacter and IP, the benefits of eradicating Helicobacter in IP and the outcome of not treating. The search strategy was based on Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines: 21 of 204 articles met the inclusion criteria. The results show that the assumption that any benefit of Helicobacter eradication results from improved levodopa bioavailability is unjustified. The inter-relationship between Helicobacter and IP is well-established. H. pylori virulence markers (associated with autoimmunity and immune tolerance) influence the risk, severity and progression of IP. The birth cohort effect for virulence marker antibodies, seen in controls, is obliterated in IP, suggesting causality. Successful H. pylori eradication in IP is disease-modifying (even in anti-parkinsonian treatment-naïve patients) but not preventive. Hypokinesia regresses with eradication and overall motor severity lessens. Eradication may influence gastrointestinal microbiota adversely, unlocking the next stage in the natural history, the development of rigidity. Failed eradication worsens hypokinesia, as does the presence/persistence of H. pylori at molecular level only. Adequate prognostic assessment of the consequences of not treating Helicobacter, for IP, is prevented by a short follow-up. We conclude that Helicobacter is a pathophysiological driver of IP.
Distinct Microbiota Dysbiosis in Patients with Non-Erosive Reflux Disease and Esophageal Adenocarcinoma
Authors: Zhou Jerry , Shrestha Prapti , Qiu Zhiguang , Harman David G. , Teoh Wun-Chung , Al-Sohaily Sam , Liem Han , Turner Ian , Ho Vincent
Published: July 2020

Non-erosive reflux disease (NERD) and esophageal adenocarcinoma (EAC) are often regarded as bookends in the gastroesophageal reflux disease spectrum. However, there is limited clinical evidence to support this disease paradigm while the underlying mechanisms of disease progression remain unclear. In this study, we used 16S rRNA sequencing and mass-spectrometer-based proteomics to characterize the esophageal microbiota and host mucosa proteome, respectively. A total of 70 participants from four patient groups (NERD, reflux esophagitis, Barrett’s esophagus, and EAC) and a control group were analyzed. Our results showed a unique NERD microbiota composition, distinct to control and other groups. We speculate that an increase in sulfate-reducing Proteobacteria and Bacteroidetes along with hydrogen producer Dorea are associated with a mechanistic role in visceral hypersensitivity. We also observed a distinct EAC microbiota consisting of a high abundance of lactic acid-producing bacteria (Staphylococcus, Lactobacillus, Bifidobacterium, and Streptococcus), which may contribute towards carcinogenesis through dysregulated lactate metabolism. This study suggests the close relationship between esophageal mucosal microbiota and the appearance of pathologies of this organ.
Prognostic Implications of 18-FDG Positron Emission Tomography/Computed Tomography in Resectable Pancreatic Cancer
Authors: Sperti Cosimo , Friziero Alberto , Serafini Simone , Bissoli Sergio , Ponzoni Alberto , Grego Andrea , Grego Emanuele , Moletta Lucia
Published: July 2020

There are currently no known preoperative factors for determining the prognosis in pancreatic cancer. The aim of this study was to examine the role of 18-fluorodeoxyglucose (18-FDG) positron emission tomography/computed tomography (18-FDG-PET/CT) as a prognostic factor for patients with resectable pancreatic cancer. Data were obtained from a retrospective analysis of patients who had a preoperative PET scan and then underwent pancreatic resection from January 2007 to December 2015. The maximum standardized uptake value (SUVmax) of 18-FDG-PET/CT was calculated. Patients were divided into high (>3.65) and low (≤3.65) SUVmax groups, and compared in terms of their TNM classification (Union for International Cancer Contro classification), pathological grade, surgical treatment, state of resection margins, lymph node involvement, age, sex, diabetes and serum Carbohydrate Antigen 19-9 (CA 19-9) levels. The study involved 144 patients, 82 with high SUVmax pancreatic cancer and 62 with low SUVmax disease. The two groups’ disease-free and overall survival rates were significantly influenced by tumor stage, lymph node involvement, pathological grade, resection margins and SUVmax. Patients with an SUVmax ≤ 3.65 had a significantly better survival than those with SUVmax > 3.65 (p < 0.001). The same variables were independent predictors of survival on multivariate analysis. The SUVmax calculated with 18-FDG-PET/CT is an important prognostic factor for patients with pancreatic cancer, and may be useful in decisions concerning patients’ therapeutic management.
Use of Guselkumab for the Treatment of Moderate-to-Severe Plaque Psoriasis: A 1 Year Real-Life Study
Authors: Galluzzo Marco , Tofani Lorenzo , Lombardo Paolo , Petruzzellis Alessandra , Silvaggio Dionisio , Egan Colin Gerard , Bianchi Luca , Talamonti Marina
Published: July 2020

Little information is available from real-life studies evaluating the efficacy of guselkumab in moderate-to-severe psoriasis. In this real-life study, we retrospectively examined a database of 52 patients with moderate-to-severe psoriasis treated with guselkumab (100 mg, s.c.) and followed for 1 year. Disease severity and treatment response was assessed by the Psoriasis Area and Severity Index (PASI) at baseline and after 4, 12, 20, 28, 36, 44, and 52 weeks. Predictors of a PASI response were evaluated by univariate and multivariate regression. After 12 months, 84.2% of patients (mean age 51.3 ± 14.1 years) treated with guselkumab achieved a PASI score of <3. Furthermore, PASI score decreased from 20 ± 13.3 at baseline to 4.4 ± 4.7 and 2.7 ± 3.9 at 12 and 20 weeks, and PASI 75, 90, and 100 response was achieved in 84.2%, 78.9%, and 63.2% of patients respectively at 12 months. Stepwise multivariate regression analysis revealed that previous biological treatment and the presence of comorbidities were associated with poorer response between 28–44 weeks, however the presence of obesity per se was not associated with poorer response. Difficult-to-treat areas were also improved as early as 12 weeks following guselkumab. Guselkumab was observed to be effective and safe in patients with moderate-severe chronic psoriasis in a real world-setting.
Positive Fecal Immunochemical Test Results Are Associated with Increased Risks of Esophageal, Stomach, and Small Intestine Cancers
Authors: Jung Yoon Suk , Lee Jinhee , Moon Chang Mo
Published: July 2020

Background: The current guideline does not recommend upper gastrointestinal evaluation for patients with a positive fecal immunochemical test (FIT) and negative colonoscopy results. However, this indication was based on low-quality evidence as data on this issue are very limited. We assessed the risk of proximal cancers (oral or throat, esophageal, stomach, and small intestine cancers) after negative or positive FIT results in the Korean National Cancer Screening Program (NCSP). Methods: Using the NCSP databases, we collected data on participants who underwent FIT between 2009 and 2011. Participants were classified based on FIT results and colorectal cancer (CRC) diagnosed within 1 year after FIT as FIT− (n = 5,551,755), FIT+/CRC− (n = 368,553), and FIT+/CRC+ (n = 12,236). Results: The incidence rates of overall proximal cancers in FIT−, FIT+/CRC−, and FIT+/CRC+ patients within 1, 2, and 3 years after FIT were 0.38%, 0.68%, and 2.26%; 0.57%, 0.93%, and 2.74%; and 0.79%, 1.21%, and 3.15%, respectively. After adjusting confounding variables, the risks of esophageal, stomach, and small intestine cancers as well as overall proximal cancers within 1, 2, and 3 years after FIT were higher in FIT+/CRC− patients than those in FIT− patients. However, the risk of oral or throat cancer did not differ between FIT− and FIT+/CRC− patients. The risks for oral or throat cancer and small intestine cancer were higher in FIT+/CRC+ patients than those in FIT+/CRC− patients. Conclusions: In this population-based study, FIT+/CRC− patients were at higher risk for esophageal, stomach, and small intestine cancers than were FIT− patients, suggesting that positive FIT results were associated with these cancers.
Carbon Nano Onions–Polystyrene Composite for Sensing S-Containing Amino Acids
Authors: Babar Dipak Gorakh , Gupta Nidhi Rani , Nandi Goutam , Sarkar Sabyasachi
Published: July 2020

A carbon nano-onions (CNOs)–polystyrene (PS) composite-based Pt electrode was used for the voltammetric detection of cysteine (Cys) and methionine (Met). The electrochemical behaviors of Cys and Met were analyzed with Cyclic Voltammetry (CV) and Differential Pulse (DP) Voltammetry at different pHs. The modified CNOs–PS/Pt electrode shows an oxidation peak at +0.4V for Cys and +0.8V for Met, respectively. Admirable sensitivity, easy fabrication, and reproducible performance make the proposed electrode well functional and useful for the qualitative and quantitative detection of sulphur-containing amino acids.
A Synthetic Peptide, CK2.3, Inhibits RANKL-Induced Osteoclastogenesis through BMPRIa and ERK Signaling Pathway
Authors: Nguyen John , Kelly Semaj , Wood Ryan , Heubel Brian , Nohe Anja
Published: July 2020

The skeletal system plays an important role in the development and maturation process. Through the bone remodeling process, 10% of the skeletal system is renewed every year. Osteoblasts and osteoclasts are two major bone cells that are involved in the development of the skeletal system, and their activity is kept in balance. An imbalance between their activities can lead to diseases such as osteoporosis that are characterized by significant bone loss due to the overactivity of bone-resorbing osteoclasts. Our laboratory has developed a novel peptide, CK2.3, which works as both an anabolic and anti-resorptive agent to induce bone formation and prevent bone loss. We previously reported that CK2.3 mediated mineralization and osteoblast development through the SMAD, ERK, and AKT signaling pathways. In this study, we demonstrated the mechanism by which CK2.3 inhibits osteoclast development. We showed that the inhibition of MEK by the U0126 inhibitor rescued the osteoclast development of RAW264.7 induced by RANKL in a co-culture system with CK2.3. We observed that CK2.3 induced ERK activation and BMPRIa expression on Day 1 after stimulation with CK2.3. While CK2.3 was previously reported to induce the SMAD signaling pathway in osteoblast development, we did not observe any changes in SMAD activation in osteoclast development with CK2.3 stimulation. Understanding the mechanism by which CK2.3 inhibits osteoclast development will allow CK2.3 to be developed as a new treatment for osteoporosis.
Identification of QR Code Perspective Distortion Based on Edge Directions and Edge Projections Analysis
Authors: Karrach Ladislav , Pivarčiová Elena , Božek Pavol
Published: July 2020

QR (quick response) Codes are one of the most popular types of two-dimensional (2D) matrix codes currently used in a wide variety of fields. Two-dimensional matrix codes, compared to 1D bar codes, can encode significantly more data in the same area. We have compared algorithms capable of localizing multiple QR Codes in an image using typical finder patterns, which are present in three corners of a QR Code. Finally, we present a novel approach to identify perspective distortion by analyzing the direction of horizontal and vertical edges and by maximizing the standard deviation of horizontal and vertical projections of these edges. This algorithm is computationally efficient, works well for low-resolution images, and is also suited to real-time processing.
An Experimental Investigation of Wave Forces on a Submerged Horizontal Plate over a Simple Slope
Authors: Dong Jie , Xue Leiping , Cheng Kaiyu , Shi Jian , Zhang Chi
Published: July 2020

We experimentally investigated the forces induced by monochromatic and solitary waves on a submerged horizontal plate in a wave flume. The experimental results of two-dimensional wave forces on the plate over a 1:10 simple slope and a flat bottom are presented. The effects of the uneven bottom on wave loads are discussed by comparing the results with those in a constant water depth. The measured nonlinear wave forces exhibited considerable discrepancies with the theoretical results from the linear wave theory. The wave forces on the plate induced by monochromatic waves over the simple slope in intermediate water showed no appreciable difference with the flat-bottom results. The solitary wave forces in terms of the downward vertical force and overturning moment significantly decreased in the existence of the simple slope. Furthermore, the dependency of the wave length, wave height and the submergence depth on the wave loads is also discussed.
High Throughput Preparation of Poly(Lactic-Co-Glycolic Acid) Nanoparticles Using Fiber Fluidic Reactor
Authors: Heshmati Aghda Niloofar , Lara Emilio J. , Patel Pulinkumar , Betancourt Tania
Published: July 2020

Polymeric nanoparticles (NPs) have a variety of biomedical, biotechnology, agricultural and environmental applications. As such, a great need has risen for the fabrication of these NPs in large scales. In this study, we used a high throughput fiber reactor for the preparation of poly(lactic-co-glycolic acid) (PLGA) NPs via nanoprecipitation. The fiber reactor provided a high surface area for the controlled interaction of an organic phase containing the PLGA solution with an aqueous phase, containing poly(vinyl alcohol) (PVA) as a stabilizer. This interaction led to the self-assembly of the polymer into the form of NPs. We studied operational parameters to identify the factors that have the greatest influence on the properties of the resulting PLGA NPs. We found that the concentration of the PLGA solution is the factor that has the greatest effect on NP size, polydispersity index (PDI), and production rate. Increasing PLGA concentration increased NP sizes significantly, while at the same time decreasing the PDI value. The second factor that was found to affect NP properties was the concentration of PVA solution, which resulted in increased NP sizes and decreased production rates. Flowrates of the feed streams also affected NP size to a lesser extent, while changing the operational temperature did not change the product’s features. In general, the results demonstrate that fiber reactors are a suitable method for the large-scale, continuous preparation of polymeric NPs suitable for biomedical applications.
A Comparative Analysis of Forming Railway Axles in 3- and 4-Roll Rolling Mills
Authors: Pater Zbigniew
Published: July 2020

The paper presents a comparative analysis of skew rolling in 3- and 4-roll CNC rolling mills. The analysis is performed using the FEM-based commercial simulation software Simufact.Forming. The formation of both solid and hollow railway axles is analyzed. Distributions of effective strain, temperature and damage criterion in rolled axles are determined, and loads and torques acting on the tools during rolling are estimated. An innovative concept of calibrating hollow axles by the rotary compression technique developed at the Lublin University of Technology is presented. Experimental tests of rolling solid axles in a 3-roll rolling mill (in a scale of 1:5) are performed, and basic force parameters of the rolling process are measured. Numerical and experimental loads and torques show a high agreement in terms of both quality and quantity.
Direct Observations of the Structural Properties of Semiconducting Polymer: Fullerene Blends under Tensile Stretching
Authors: Aliouat Mouaad Yassine , Ksenzov Dmitriy , Escoubas Stephanie , Ackermann Jörg , Thiaudière Dominique , Mocuta Cristian , Benoudia Mohamed Cherif , Duche David , Thomas Olivier , Grigorian Souren
Published: July 2020

We describe the impact of tensile strains on the structural properties of thin films composed of PffBT4T-2OD π-conjugated polymer and PC71BM fullerenes coated on a stretchable substrate, based on a novel approach using in situ studies of flexible organic thin films. In situ grazing incidence X-ray diffraction (GIXD) measurements were carried out to probe the ordering of polymers and to measure the strain of the polymer chains under uniaxial tensile tests. A maximum 10% tensile stretching was applied (i.e., beyond the relaxation threshold). Interestingly we found different behaviors upon stretching the polymer: fullerene blends with the modified polymer; fullerene blends with the 1,8-Diiodooctane (DIO) additive. Overall, the strain in the system was almost twice as low in the presence of additive. The inclusion of additive was found to help in stabilizing the system and, in particular, the π–π packing of the donor polymer chains.
Insights into Characterization Methods and Biomedical Applications of Nanoparticle–Protein Corona
Authors: Li Yan , Lee Jae-Seung
Published: July 2020

Nanoparticles (NPs) exposed to a biological milieu will strongly interact with proteins, forming “coronas” on the surfaces of the NPs. The protein coronas (PCs) affect the properties of the NPs and provide a new biological identity to the particles in the biological environment. The characterization of NP-PC complexes has attracted enormous research attention, owing to the crucial effects of the properties of an NP-PC on its interactions with living systems, as well as the diverse applications of NP-PC complexes. The analysis of NP-PC complexes without a well-considered approach will inevitably lead to misunderstandings and inappropriate applications of NPs. This review introduces methods for the characterization of NP-PC complexes and investigates their recent applications in biomedicine. Furthermore, the review evaluates these characterization methods based on comprehensive critical views and provides future perspectives regarding the applications of NP-PC complexes.
A Modular Machine-Learning-Based Approach to Improve Tensile Properties Uniformity Along Hot Dip Galvanized Steel Strips for Automotive Applications
Authors: Colla Valentina , Cateni Silvia , Maddaloni Alessandro , Vignali Antonella
Published: July 2020

The paper presents a machine learning-based system aimed at improving the homogeneity of tensile properties of steel strips for automotive applications over their strip length in the annealing and hot dip galvanizing lines. A novel modular approach is proposed exploiting process and product data and combining smart data pre-processing and cleansing algorithms, an ensemble of neural networks targeted to specific product classes and an ad-hoc developed iterative procedure for identifying the variability ranges of the most relevant process variables. A decision support concept is implemented through a software tool, which facilitates exploitation by plant managers and operators. The system has been tested on site. The results show its effectiveness in improving the control of the thermal evolution of the strip with respect to the standard operating practice.
Application of the Cumulative Kinetic Model in the Comminution of Critical Metal Ores
Authors: Ciribeni Victor , Bertero Regina , Tello Andrea , Puerta Matías , Avellá Enzo , Paez Matías , Menéndez Aguado Juan María
Published: July 2020

Over the last decades, several reliable mathematical models have been developed for simulating ore comminution processes and determining the Work Index. Since Fred Chester Bond developed the Work Index standard procedure in 1961, numerous attempts have been made to find simpler, faster, and economically more advantageous alternative tests. In this paper, a Bond test simulation based on the cumulative kinetic model (CKM) has been checked on a spreadsheet. The research has been accomplished by conventionally determining the kinetic parameters for some Ag and Au ores and for three pure minerals and one rock that are common constituents of the gangue rock. Analysis of the results obtained allowed to develop a simplified procedure for calculating the kinetic parameters and their application to Work Index determination through simulation.
Mössbauer and X-ray Studies of Phase Composition of Fly Ashes Formed after Combustion of Ekibastuz Coal (Kazakhstan)
Authors: Shokanov Adilkhan , Vereshchak Mikhail , Manakova Irina
Published: July 2020

Mössbauer spectroscopy and X-ray diffraction have been used to study samples of fly ashes formed after combustion of coal from the Ekibastuz basin at the thermal power plants TPP-2 and TPP-3 in Almaty (Kazakhstan). It has been established that the fractions of fly ashes contain iron in the form of magnetite Fe3O4 and hematite α-Fe2O3. The mixed valence of iron Fe3+ ↔ Fe2+ in the octahedral sublattice of magnetite is destroyed by isostructural substitution impurities. Maghemite γ-Fe2O3 is additionally present in the fly ash of TPP-3 as a product of magnetite slow oxidation. It was shown that at T ≥ 1400 °C the proportion of magnetite in fly ashes increases due to decomposition of hematite, maghemite, hercynite and the drop of iron content in mullite. It was concluded that the amount of iron in magnetite is a temperature indicator of fly ashes formation. The parameters of hyperfine interactions have been determined in the iron-containing minerals of fly ashes. It was identified that formation of the fly ashes structure occurs in oxidizing atmosphere, since no traces were revealed of reducing environment effect on the phase composition.
Design and Implementation of a Multifunction Wearable Device to Monitor Sleep Physiological Signals
Authors: Liao Lun-De , Wang Yuhling , Tsao Yung-Chung , Wang I-Jan , Jhang De-Fu , Chuang Chiung-Cheng , Chen Sheng-Fu
Published: July 2020

We present a wearable device built on an Adafruit Circuit Playground Express (CPE) board and integrated with a photoplethysmographic (PPG) optical sensor for heart rate monitoring and multiple embedded sensors for medical applications—in particular, sleep physiological signal monitoring. Our device is portable and lightweight. Due to the microcontroller unit (MCU)-based architecture of the proposed device, it is scalable and flexible. Thus, with the addition of different plug-and-play sensors, it can be used in many applications in different fields. The innovation introduced in this study is that with additional sensors, we can determine whether there are intermediary variables that can be modified to improve our sleep monitoring algorithm. Additionally, although the proposed device has a relatively low cost, it achieves substantially improved performance compared to the commercially available Philips ActiWatch2 wearable device, which has been approved by the Food and Drug Administration (FDA). To assess the reliability of our device, we compared physiological sleep signals recorded simultaneously from volunteers using both our device and ActiWatch2. Motion and light detection data from our device were shown to be correlated to data simultaneously collected using the ActiWatch2, with correlation coefficients of 0.78 and 0.89, respectively. For 7 days of continuous data collection, there was only one instance of a false positive, in which our device detected a sleep interval, while the ActiWatch2 did not. The most important aspect of our research is the use of an open architecture. At the hardware level, general purpose input/output (GPIO), serial peripheral interface (SPI), integrated circuit (I2C), and universal asynchronous receiver-transmitter (UART) standards were used. At the software level, an object-oriented programming methodology was used to develop the system. Because the use of plug-and-play sensors is associated with the risk of adverse outcomes, such as system instability, this study heavily relied on object-oriented programming. Object-oriented programming improves system stability when hardware components are replaced or upgraded, allowing us to change the original system components at a low cost. Therefore, our device is easily scalable and has low commercialization costs. The proposed wearable device can facilitate the long-term tracking of physiological signals in sleep monitoring and related research. The open architecture of our device facilitates collaboration and allows other researchers to adapt our device for use in their own research, which is the main characteristic and contribution of this study.
Electrical Properties of Ultra-Fast 3D-Trench Electrode Silicon Detector
Authors: Liu Manwen , Zhou Tao , Li Zheng
Published: July 2020

In our previous work on ultra-fast silicon detectors, extremely small carrier drift times of 50–100 picoseconds were predicted for electrode spacing of 5–10 μm. Expanding on these previous works, we systematically study the electrical characteristics of the ultra-fast, 3D-trench electrode silicon detector cell with p-type bulk silicon, such as electric potential distribution, electric field distribution, hole concentration distribution, and leakage current to analyze the full detector depletion voltage and other detector properties. To verify the prediction of ultra-fast response times, we simulate the instant induced current curves before and after irradiation with different minimum ionizing particle (MIP) hitting positions. High position resolution pixel detectors can be fabricated by constructing an array of these extremely small detector cells.
Helicobacter pylori Colonization Drives Urokinase Receptor (uPAR) Expression in Murine Gastric Epithelium During Early Pathogenesis
Authors: Alpízar-Alpízar Warner , Skindersoe Mette E. , Rasmussen Lone , Kriegbaum Mette C. , Christensen Ib J. , Lund Ida K. , Illemann Martin , Laerum Ole D. , Krogfelt Karen A. , Andersen Leif P. , Ploug Michael
Published: July 2020

(1) Background: Persistent Helicobacter pylori infection is the most important risk factor for gastric cancer. The urokinase receptor (uPAR) is upregulated in lesions harboring cancer invasion and inflammation. Circumstantial evidence tends to correlate H. pylori colonization with increased uPAR expression in the human gastric epithelium, but a direct causative link has not yet been established in vivo; (2) Methods: In a mouse model of H. pylori-induced gastritis, we investigated the temporal emergence of uPAR protein expression in the gastric mucosa in response to H. pylori (SS1 strain) infection; (3) Results: We observed intense uPAR immunoreactivity in foveolar epithelial cells of the gastric corpus due to de novo synthesis, compared to non-infected animals. This uPAR induction represents a very early response, but it increases progressively over time as do infiltrating immune cells. Eradication of H. pylori infection by antimicrobial therapy causes a regression of uPAR expression to its physiological baseline levels. Suppression of the inflammatory response by prostaglandin E2 treatment attenuates uPAR expression. Notwithstanding this relationship, H. pylori does induce uPAR expression in vitro in co-cultures with gastric cancer cell lines; (4) Conclusions: We showed that persistent H. pylori colonization is a necessary event for the emergence of a relatively high uPAR protein expression in murine gastric epithelial cells.
Experimental Procedure for the Determination of the Critical Coalescence Concentration (CCC) of Simple Frothers
Authors: Guven Onur , Batjargal Khandjamts , Ozdemir Orhan , Karakashev Stoyan I. , Grozev Nikolay A. , Boylu Feridun , Çelik Mehmet Sabri
Published: July 2020

In this study, the critical coalescence concentrations (CCC) of selected commercial frother solutions, namely polypropylene glycols (PPG 200, 400, and 600), tri propylene glycol (BTPG), triethylene glycol (BTEG), dipropylene glycol (BDPG), and as a reference, methyl isobutyl carbinol (MIBC), were determined using a bubble column based on light absorption. The results for all seven frothers showed that BTEG has the worst bubble inhibiting performance, and PPG 600 has the best bubble inhibiting performance. While critical coalescence concentration (CCC) was found as 3 ppm for PPG 600, it increased to 25 ppm for BTEG. In the case of MIBC, which was the reference point, the CCC value was found as 10 ppm, which was consistent with the literature. The surface tension isotherms of the frothers were determined and analyzed with one of the latest adsorption models. The results indicated that the polypropylene glycol frothers showed more surface activity compared to alcohol or other frothers investigated. This is due to the additional reorganization of the PPG molecules on the air/water interface, thus boosting its surface activity.
Acemannan Used as an Implantable Biomaterial for Vital Pulp Therapy of Immature Permanent Teeth Induced Continued Root Formation
Authors: Vu Tien Thuy , Nguyen Minh Truong , Sangvanich Polkit , Nguyen Quang Ngoc , Thunyakitpisal Pasutha
Published: July 2020

Direct pulp-capping, a vital pulp therapy, is used to protect and preserve pulp vitality by applying a biomaterial on the pulp exposure site. Acemannan, a polysaccharide extracted from Aloe vera, induces osteodentin-bridge formation to cover the exposure site in vivo. We evaluated the effect of acemannan sponges on partial pulpotomized permanent teeth with caries or accident-induced pulp exposure (n = 50). After removing infected dentin and inflamed pulp tissue, the teeth were randomly divided into acemannan or control (mineral trioxide aggregate (MTA) groups (n = 25). The teeth were examined immediately after treatment (baseline) and at 6- and 12-month follow-ups for clinical and cone beam computed tomography (CBCT) examination. The three-dimensional tooth length and root apex area were simulated to determine treatment success. We found that the overall success rate in the acemannan and MTA groups from baseline to 12-month follow-up was 90.91% and 95.65%, respectively, with no significant difference between the two groups (p > 0.05). In the success teeth in both groups, the root length increased, and the apex area significantly decreased (p < 0.05), indicating continued root formation. Our results suggest that acemannan is a promising low-cost biomaterial for partial pulpotomy treatment for immature permanent teeth requiring vital pulp therapy.
Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents
Authors: Kumar Kulabhusan Prabir , Hussain Babar , Yüce Meral
Published: July 2020

Aptamers are synthetic single-stranded DNA or RNA sequences selected from combinatorial oligonucleotide libraries through the well-known in vitro selection and iteration process, SELEX. The last three decades have witnessed a sudden boom in aptamer research, owing to their unique characteristics, like high specificity and binding affinity, low immunogenicity and toxicity, and ease in synthesis with negligible batch-to-batch variation. Aptamers can specifically bind to the targets ranging from small molecules to complex structures, making them suitable for a myriad of diagnostic and therapeutic applications. In analytical scenarios, aptamers are used as molecular probes instead of antibodies. They have the potential in the detection of biomarkers, microorganisms, viral agents, environmental pollutants, or pathogens. For therapeutic purposes, aptamers can be further engineered with chemical stabilization and modification techniques, thus expanding their serum half-life and shelf life. A vast number of antagonistic aptamers or aptamer-based conjugates have been discovered so far through the in vitro selection procedure. However, the aptamers face several challenges for its successful clinical translation, and only particular aptamers have reached the marketplace so far. Aptamer research is still in a growing stage, and a deeper understanding of nucleic acid chemistry, target interaction, tissue distribution, and pharmacokinetics is required. In this review, we discussed aptamers in the current diagnostics and theranostics applications, while addressing the challenges associated with them. The report also sheds light on the implementation of aptamer conjugates for diagnostic purposes and, finally, the therapeutic aptamers under clinical investigation, challenges therein, and their future directions.
Effectiveness of a Controlled 5-FU Delivery Based on FZD10 Antibody-Conjugated Liposomes in Colorectal Cancer In vitro Models
Authors: Scavo Maria Principia , Cutrignelli Annalisa , Depalo Nicoletta , Fanizza Elisabetta , Laquintana Valentino , Gasparini Giampietro , Giannelli Gianluigi , Denora Nunzio
Published: July 2020

The use of controlled delivery therapy in colorectal cancer (CRC) reduces toxicity and side effects. Recently, we have suggested that the Frizzled 10 (FZD10) protein, a cell surface receptor belonging to the FZD protein family that is overexpressed in CRC cells, is a novel candidate for targeting and treatment of CRC. Here, the anticancer effect of novel immuno-liposomes loaded with 5-Fluorouracil (5-FU), decorated with an antibody against FZD10 (anti-FZD10/5-FU/LPs), was evaluated in vitro on two different CRC cell lines, namely metastatic CoLo-205 and nonmetastatic CaCo-2 cells, that were found to overexpress FZD10. The anti-FZD10/5-FU/LPs obtained were extensively characterized and their preclinical therapeutic efficacy was evaluated with the MTS cell proliferation assay based on reduction of tetrazolium compound, scratch test, Field Emission Scanning Electron Microscopes (FE-SEM) investigation and immunofluorescence analysis. The results highlighted that the cytotoxic activity of 5-FU was enhanced when encapsulated in the anti-FZD10 /5-FU/LPs at the lowest tested concentrations, as compared to the free 5-FU counterparts. The immuno-liposomes proposed herein possess a great potential for selective treatment of CRC because, in future clinical applications, they can be encapsulated in gastro-resistant capsules or suppositories for oral or rectal delivery, thereby successfully reaching the intestinal tract in a minimally invasive manner.
Improving Nefiracetam Dissolution and Solubility Behavior Using a Cocrystallization Approach
Authors: Buol Xavier , Robeyns Koen , Caro Garrido Camila , Tumanov Nikolay , Collard Laurent , Wouters Johan , Leyssens Tom
Published: July 2020

In this work, we are the first to identify thirteen cocrystals of Nefiracetam, a poor water-soluble nootropic compound. Three of which were obtained with the biocompatible cocrystallization agents citric acid, oxalic acid, and zinc chloride. These latter have been fully structurally and physically characterized and the solubility, dissolution rate, and stability were compared to that of the initial Active Pharmaceutical Ingredient (API).
Improved Self-Supporting and Ceramifiable Properties of Ceramifiable EPDM Composites by Adding Aramid Fiber
Authors: Zhao Dong , Liu Wei , Shen Yucai , Jiang Guodong , Wang Tingwei
Published: July 2020

Ceramifiable ethylene propylene diene monomer (EPDM) composites with fiber network structures were prepared by using aramid fiber (AF), ammonium polyphosphate (APP), and silicate glass frits (SGF). The effect of AF on the curing characteristic of the ceramifiable EPDM composites was studied. The morphology of AF in the composites system was observed by optical microscopy (OM) and scanning electron microscope (SEM). The effects of the observed AF network structures on the solvent resistance, mechanical properties, ablative resistance, self-supporting property, and ceramifiable properties of the composites were investigated. Results suggested that the existence of the AF network structure improved the vulcanization properties, solvent resistance, thermal stability, and ablative resistance of the EPDM composites. An excellent self-supporting property of the EPDM composites was obtained by combining the formation of the AF network and the formation of crystalline phases at higher temperature (above 600 °C). The thermal shrinkage performance of AF and the increased thermal stability of the EPDM composites improved the ceramifiable properties of the EPDM composites.
Investigation of the Mechanical and Dynamic-Mechanical Properties of Electrospun Polyvinylpyrrolidone Membranes: A Design of Experiment Approach
Authors: Dodero Andrea , Brunengo Elisabetta , Castellano Maila , Vicini Silvia
Published: July 2020

Polyvinylpyrrolidone electrospun membranes characterized by randomly, partially, or almost completely oriented nanofibers are prepared using a drum collector in static (i.e., 0 rpm) or rotating (i.e., 250 rpm or 500 rpm) configuration. Besides a progressive alignment alongside the tangential speed direction, the nanofibers show a dimension increasing with the collector rotating speed in the range 410–570 nm. A novel design of experiment approach based on a face-centred central composite design is employed to describe membrane mechanical properties using the computation of mathematical models and their visualization via response surface methodology. The results demonstrate the anisotropic nature of the fibre-oriented membranes with Young’s modulus values of 165 MPa and 71 MPa parallelly and perpendicularly to the alignment direction, respectively. Above all, the proposed approach is proved to be a promising tool from an industrial point of view to prepare electrospun membranes with a tailored mechanical response by simply controlling the collector speed.
Study of Compaction Properties and Permeability Prediction of Multilayered Quadriaxial Non-Crimp Fabric in Liquid Composite Molding Process
Authors: Geng Yi , Jiang Jinhua , Lin Fangbing , Shao Huiqi , Zhang Chenglong , Chen Nanliang
Published: July 2020

A systematic experimental study was performed to detect the compaction and permeability properties of multilayered biaxial and quadriaxial preforms under vacuum pressure. Compression response on ply level showed that the degree of nesting between quadriaxial NCF was more pronounced and the nesting deformation mechanism was affected by the interaction with stitch yarns. Owing to the meso-channels in the fibrous structure and the nesting between layers, the in-plane permeability of quadriaxial NCF did not follow an inverse proportion relationship with the fiber volume fraction. To predict the in-plane permeability of multilayered quadriaxial NCFs, unit cell models at a high level of geometrical details were built, including local variations in yarn cross-sections and the nesting deformation between layers. Numerical methods were implemented, and the prediction results were in very good agreement with the experimental data. Besides, the major contributing parameters to the enhancement of the in-plane permeabilities were identified by investigating the correlation between permeability and structural parameters of quadriaxial NCF. The modeling methodology and the principles established can be applied to the design of the quadriaxial NCF fabrics, where the permeability enhancement was evidenced.
Eco-Conversion of Two Winery Lignocellulosic Wastes into Fillers for Biocomposites: Vine Shoots and Wine Pomaces
Authors: David Grégoire , Vannini Micaela , Sisti Laura , Marchese Paola , Celli Annamaria , Gontard Nathalie , Angellier-Coussy Hélène
Published: July 2020

Two winery residues, namely vine shoots (ViSh) and wine pomace (WiPo), were up-cycled as fillers in PHBV-based biocomposites. Answering a biorefinery approach, the impact of a preliminary polyphenols extraction step using an acetone/water mixture on the reinforcing effect of fillers was assessed. Biocomposites (filler content up to 20 wt%) were prepared by melt-mixing and compared in terms of final performance (thermal, mechanical and barrier). It was shown that the reinforcing effect was slightly better in the case of vine shoots, while it was not significantly affected by the pre-treatment, demonstrating that these two winery residues could be perfectly used as fillers in composite materials even after an extraction process to maximize their potential of valorization.
European Union Legislation Overview about Used Vegetable Oils Recycling: The Spanish and Italian Case Studies
Authors: Ibanez Jesus , Martel Martín Sonia , Baldino Salvatore , Prandi Cristina , Mannu Alberto
Published: July 2020

The employment of used vegetable oils (UVOs) as raw materials in key sectors as energy production or bio-lubricant synthesis represents one of the most relevant priorities in the European Union (EU) normative context. In many countries, the development of new production processes based on the circular economy model, as well as the definition of future energy and production targets, involve the utilization of wastes as raw material. In this context, the main currently applied EU regulations are presented and discussed. As in the EU, the general legislative process consists of the definition in each State Member of specific legislation, which transposes the EU indications. Two relevant countries are herein considered: Italy and Spain. Through the analysis of the conditions required in both countries for UVOs’ collection, disposal, storage, and recycling, a wide panorama of the current situation is provided.
Statistical Review of Microstructure-Property Correlation of Stainless Steel: Implication for Pre- and Post-Weld Treatment
Authors: Muhammed Musa , Mustapha Mazli , Ginta Turnad Lenggo , Ali Abdullah Musa , Mustapha Faizal , Hampo Chima Cyril
Published: July 2020

For the past three centuries, there has been a very high demand for stainless steel for different applications, due to its corrosion resistance coupled with the good strength and low cost of the metal. Several welding techniques have been adopted in the fabrication of stainless steel, with the choice of welding technique hinged on the desired requirements. Advancement has been made in its dissimilar welding with other metals like aluminum, copper and titanium. While similar welding of stainless steel faces the challenge of weld metal property deterioration, dissimilar welding poses more serious challenges due to the differential in chemical composition and the thermophysical properties of the base metals. A review of the literature reveals that considerable progress has been made in the improvement of the properties of the weld joint by the application of several weld treatment processes. It was discovered that most of the researchers focused on the effect of these weld treatment processes on the properties of the weld joints, with little attempt to establish a relationship between the microstructure and properties. This review paper critically analyzed the effect of weld treatment processes on the properties of stainless steel in light of microstructure-property correlation.
Being a ‘White Muslima’ in the Netherlands Ethnicity, Friendships and Relationships—The Dutch Conversion Narrative
Authors: Hass Bat Sheva
Published: July 2020

This article, which is part of a larger ongoing project, examines relationships, friendships and levels of belonging in Dutch society, as well as in the Dutch Muslim community in narratives of women converted to Islam. The ethnicity of these women is always visible as ‘native Dutch’ and shapes their conversion narratives. This ethnography raises a number of questions that form the basis for the analysis presented here: How do Dutch Muslim women shape their identity in a way that is both Dutch and Muslim? Do they incorporate Dutch parameters into their Muslim identity, while at the same time weaving Islamic principles into their Dutch sense of self? The findings show how the conversion narrative can be mobilized by Dutch Muslim women to serve identity formation, levels of belonging and personal (religious) choice in the Netherlands, where Islam is largely considered by the non-Muslim population to be a religion that is oppressive and discriminatory towards women and is associated with foreignness and being the Other. It is argued that, in the context of being Dutch and Muslim, these women express their freedom of choice, which is manifested through friendships, relationships and marriages (Islamic vs. civil), while their ethnicity and conversion experience is a visible component in their identity. In so doing, these women push the limits of the archetypal Dutch identity and are able to criticize Dutch society while simultaneously stretching the meaning of Islam and being critical of Dutch Muslim communities to craft their own hybrid identity.
The New Muslim Ethical Elite: “Silent Revolution” or the Commodification of Islam?
Authors: Roose Joshua M.
Published: July 2020

Very little research has examined the emergence of Western Muslims into the elite professions that are central to the operation of the capitalist free market and that serve as a central location of economic and political power. Less research still has examined how this is shaping citizenship among Muslims and the future of Islam in the West. These professions include finance, trade and auditing and supporting free market infrastructure including commercial law, consulting and the entrepreneurial arms of government public service. Many Muslim men and women in these professions maintain a commitment to their faith and are often at the forefront of identifying opportunities for the application of Islamic principles to the free market through the development of social engineering mechanisms such as Islamic finance and home loans, Islamic wills, marriage contracts, businesses and context-specific solutions for Muslim clients. These may have a potentially profound impact on belonging and practice for current and future generations of Western Muslims. The political and economic clout (and broader potential public appeal) of these new Muslim elites often significantly outweighs that of Imams and Sheikhs and thus challenges traditional textually based Islam. This article, grounded in empirical research, seeks to build upon very limited research looking at Muslim elites, exploring these developments with specific reference to professionals working in Islamic finance and law across the Western contexts of Australia and the United States, two countries with capitalist free markets and significant Muslim minorities.
Toxic Potential and Metabolic Profiling of Two Australian Biotypes of the Invasive Plant Parthenium Weed (Parthenium hysterophorus L.)
Authors: Bajwa Ali Ahsan , Weston Paul A. , Gurusinghe Saliya , Latif Sajid , Adkins Steve W. , Weston Leslie A.
Published: July 2020

Parthenium weed (Parthenium hysterophorus L.) is an invasive plant species in around 50 countries and a ‘Weed of National Significance’ in Australia. This study investigated the relative toxicity of the leaf, shoot and root extracts of two geographically separate and morphologically distinct biotypes of parthenium weed in Queensland, Australia. Parthenium weed exhibited higher phytotoxic, cytotoxic and photocytotoxic activity in leaf tissue extracts in contrast to shoot and root. The germination and seedling growth of a dicot species (garden cress) were inhibited more than those of a monocot species (annual ryegrass) using a phytotoxicity bioassay. The cytotoxicity of leaf extracts was assessed in a mouse fibroblast cell suspension assay and increased under high ultraviolet A(UV-A) radiation. A major secondary metabolite, parthenin, was found in abundance in leaf extracts and was positively correlated with cytotoxicity but not with photocytotoxicity or phytotoxicity. Ambrosin and chlorogenic acid were also detected and were positively correlated with germination inhibition and the inhibition of radicle elongation, respectively. In addition, other currently unidentified compounds in the leaf extracts were positively correlated with phytotoxicity, cytotoxicity and photocytotoxicity with two to three molecules strongly correlated in each case. Both parthenium weed biotypes investigated did not differ with respect to their relative toxicity, despite their reported differences in invasive potential in the field. This suggests that secondary chemistry plays a limited role in their invasion success.