bluuebunny/crossref_metadata_2025 huggingface dataset:
Multimodal Approach to Assessment of Fecal Microbiota Donors based on Three Complementary Methods
Authors: Bilinski Jaroslaw , Dziurzynski Mikolaj , Grzesiowski Pawel , Podsiadly Edyta , Stelmaszczyk-Emmel Anna , Dzieciatkowski Tomasz , Dziewit Lukasz , Basak Grzegorz W.
Published: June 2020

Methods of stool assessment are mostly focused on next-generation sequencing (NGS) or classical culturing, but only rarely both. We conducted a series of experiments using a multi-method approach to trace the stability of gut microbiota in various donors over time, to find the best method for the proper selection of fecal donors and to find “super-donor” indicators. Ten consecutive stools donated by each of three donors were used for the experiments (30 stools in total). The experiments assessed bacterial viability measured by flow cytometry, stool culturing on different media and in various conditions, and NGS (90 samples in total). There were no statistically significant differences between live and dead cell numbers; however, we found a group of cells classified as not-dead-not-alive, which may be possibly important in selection of “good” donors. Donor C, being a regular stool donor, was characterized by the largest number of cultivable species (64). Cultivable core microbiota (shared by all donors) was composed of only 16 species. ANCOM analysis of NGS data highlighted particular genera to be more abundant in one donor vs. the others. There was a correlation between the not-dead-not-alive group found in flow cytometry and Anaeroplasma found by NGS, and we could distinguish a regular stool donor from the others. In this work, we showed that combining various methods of microbiota assessment gives more information than each method separately.
Safety and Efficacy of Second Ahmed Valve Implant in Refractory Glaucoma
Authors: Posarelli Chiara , Toro Mario Damiano , Rejdak Robert , Żarnowski Tomasz , Pożarowska Dorota , Longo Antonio , Miccoli Mario , Nardi Marco , Figus Michele
Published: June 2020

Background: Refractory glaucoma still represents a challenge for ophthalmologists to manage intraocular pressure. The present study aimed to evaluate long term efficacy and safety of a second Ahmed valve implantation after the failure of a first implant in patients with refractory glaucoma and elevated intraocular pressure (IOP). Methods: Retrospective, multicenter non-comparative case series. Twenty-eight patients were retrospectively recruited between January 2011 and December 2017. Demographic data, glaucoma type, visual acuity, intraocular pressure, medical therapy, and complications were registered. Three criteria of success were established: Type 1 surgical success: IOP ≤ 15 mmHg and a reduction of IOP ≥ 40% from baseline; Type 2 surgical success: IOP ≤ 18 mmHg and a reduction of IOP ≥ 30% from baseline; and Type 3 surgical success: IOP ≤ 21 mmHg and a reduction of IOP ≥ 20% from baseline. Surgical failure has been established as IOP less than 5 mmHg or over 21 mmHg and less than a 20% reduction of IOP from baseline despite medications in two consecutive visits, light perception loss referable to glaucoma, and the necessity for further glaucoma surgery. Failure was observed in six (21%) patients. (3) Results: Mean IOP and mean glaucoma medication number significantly reduced from baseline after the second implantation, and the surgical success rate at 72 months ranged from 10% to 78% based on the different criteria of success. Failure was observed in six (21%) patients. Conclusions: This study confirmed the safety and efficacy of a second Ahmed valve implantation in patients with refractory glaucoma and elevated IOP at baseline.
Gut Microbiota: Implications in Alzheimer’s Disease
Authors: He Yixi , Li Binyin , Sun Dingya , Chen Shengdi
Published: June 2020

Alzheimer’s disease (AD), the most common cause of dementia, is a neurodegenerative disease that seriously threatens human health and life quality. The main pathological features of AD include the widespread deposition of amyloid-beta and neurofibrillary tangles in the brain. So far, the pathogenesis of AD remains elusive, and no radical treatment has been developed. In recent years, mounting evidence has shown that there is a bidirectional interaction between the gut and brain, known as the brain–gut axis, and that the intestinal microbiota are closely related to the occurrence and development of neurodegenerative diseases. In this review, we will summarize the laboratory and clinical evidence of the correlation between intestinal flora and AD, discuss its possible role in the pathogenesis, and prospect its applications in the diagnosis and treatment of AD.
Antimycobacterial Effects of Everolimus in a Human Granuloma Model
Authors: Ashley David , Hernandez Joshua , Cao Ruoqiong , To Kimberly , Yegiazaryan Aram , Abrahem Rachel , Nguyen Timothy , Owens James , Lambros Maria , Subbian Selvakumar , Venketaraman Vishwanath
Published: June 2020

Mycobacterium tuberculosis (M. tb) has been historically and is currently a threat to global public health. First-line antibiotics have been effective but proven to be burdensome as they have many potential adverse side effects. There has been a recent increase in the number of active tuberculosis (TB) cases due to a prevalence of multidrug and extensively drug-resistant strains of M. tb, and an increasing number of highly susceptible people such as those with Type 2 Diabetes (T2DM) and human immunodeficiency virus (HIV) infection. Multidrug-resistant M. tb infection (MDR-TB) is challenging to treat with existing therapeutics, so novel therapeutics and treatment strategies must be developed. Host-Directed Therapy (HDT) has been a potential target mechanism for effective clearance of infection. Host cell autophagy plays an essential role in antibacterial defense. The mammalian target of rapamycin (mTOR) has been negatively correlated with autophagy induction. Everolimus is an mTOR inhibitor that induces autophagy, but with higher water solubility. Therefore, targeting the mTOR pathway has the potential to develop novel and more effective combination drug therapy for TB. This study tested the effect of everolimus, alone and in combination with current first-line antibiotics (isoniazid and pyrazinamide), on the inhibition of M. tb inside in vitro human granulomas. We found that M. tb-infected in vitro granulomas treated with everolimus alone resulted in significantly decreased M. tb burden compared to similar granulomas in the control group. Cells treated with everolimus doses of either 1 nM or 2 nM in conjunction with pyrazinamide (PZA) produced a significant reduction in intracellular M. tb burden. Treatment groups that received everolimus alone in either 1 nM or 2 nM doses experienced a significant reduction in oxidative stress. Additionally, samples treated with 2 nM everolimus alone were observed to have significantly higher levels of autophagy and mTOR inhibition as well. Results from this study indicate that everolimus is efficacious in controlling M. tb infection in the granulomas and has additive effects when combined with the anti-TB drugs, isoniazid and pyrazinamide. This study has shown that everolimus is a promising host-directed therapeutic in the context of in vitro granuloma M. tb infection. Further study is warranted to better characterize these effects.
Effects of Bag Mask Ventilation and Advanced Airway Management on Adherence to Ventilation Recommendations and Chest Compression Fraction: A Prospective Randomized Simulator-Based Trial
Authors: Vogt Lea , Sellmann Timur , Wetzchewald Dietmar , Schwager Heidrun , Russo Sebastian , Marsch Stephan
Published: June 2020

The role of advanced airway management (AAM) in cardiopulmonary resuscitation (CPR) is currently debated as observational studies reported better outcomes after bag-mask ventilation (BMV), and the only prospective randomized trial was inconclusive. Adherence to CPR guidelines ventilation recommendations is unknown and difficult to assess in clinical trials. This study compared AAM and BMV with regard to adherence to ventilation recommendations and chest compression fractions in simulated cardiac arrests. A total of 154 teams of 3–4 physicians were randomized to perform CPR with resuscitation equipment restricting airway management to BMV only or equipment allowing for all forms of AAM. BMV teams ventilated 6 ± 6/min and AAM teams 19 ± 8/min (range 3–42/min; p < 0.0001 vs. BMV). 68/78 BMV teams and 23/71 AAM teams adhered to the ventilation recommendations (p < 0.0001). BMV teams had lower compression fractions than AAM teams (78 ± 7% vs. 86 ± 6%, p < 0.0001) resulting entirely from higher no-flow times for ventilation (9 ± 4% vs. 3 ± 3 %; p < 0.0001). Compared to BMV, AAM leads to significant hyperventilation and lower adherence to ventilation recommendations but favourable compression fractions. The cumulative effect of deviations from ventilation recommendations has the potential to blur findings in clinical trials.
Bioactive Surfaces vs. Conventional Surfaces in Titanium Dental Implants: A Comparative Systematic Review
Authors: López-Valverde Nansi , Flores-Fraile Javier , Ramírez Juan Manuel , Macedo de Sousa Bruno , Herrero-Hernández Silvia , López-Valverde Antonio
Published: June 2020

Animal studies and the scarce clinical trials available that have been conducted suggest that bioactive surfaces on dental implants could improve the osseointegration of such implants. The purpose of this systematic review was to compare the effectiveness of osseointegration of titanium (Ti) dental implants using bioactive surfaces with that of Ti implants using conventional surfaces such as sandblasted large-grit acid-etched (SLA) or similar surfaces. Applying the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement, the MEDLINE, PubMed Central and Web of Science databases were searched for scientific articles in April 2020. The keywords used were “dental implants”, “bioactive surfaces”, “biofunctionalized surfaces”, and “osseointegration”, according to the question: “Do bioactive dental implant surfaces have greater osseointegration capacity compared with conventional implant surfaces?” Risk of bias was assessed using the Cochrane Collaboration tool. 128 studies were identified, of which only 30 met the inclusion criteria: 3 clinical trials and 27 animal studies. The average STROBE (STrengthening the Reporting of OBservational studies in Epidemiology) and ARRIVE (Animal Research: Reporting of In Vivo Experiments) scores were 15.13 ± 2.08 and 17.7±1.4, respectively. Implant stability quotient (ISQ) was reported in 3 studies; removal torque test (RTT)—in 1 study; intraoral periapical X-ray and microcomputed tomography radiological evaluation (RE)—in 4 studies; shear force (SF)—in 1 study; bone-to-implant contact (BIC)—in 12 studies; and BIC and bone area (BA) jointly—in 5 studies. All animal studies reported better bone-to-implant contact surface for bioactive surfaces as compared to control implants with a statistical significance of p < 0.05. Regarding the bioactive surfaces investigated, the best results were yielded by the one where mechanical and chemical treatment methods of the Ti surfaces were combined. Hydroxyapatite (HA) and calcium–phosphate (Ca–Ph) were the most frequently used bioactive surfaces. According to the results of this systematic review, certain bioactive surfaces have a positive effect on osseointegration, although certain coating biomolecules seem to influence early peri-implant bone formation. Further and more in-depth research in this field is required to reduce the time needed for osseointegration of dental implants.
Immunomodulation by an Omega-6 Fatty Acid Reduced Mixed Lipid Emulsion in Murine Acute Respiratory Distress Syndrome
Authors: Hecker Matthias , Rose Matthias , Hecker Andreas , Dietrich Hartmut , Schaefer Martina B. , Sommer Natascha , Seeger Werner , Mayer Konstantin
Published: June 2020

Background: Acute respiratory distress syndrome (ARDS) is associated with both high morbidity and mortality in intensive care units worldwide. Patients with ARDS often require parenteral nutrition with lipid emulsions as essential components. In the present study, we assessed the immunomodulatory and apoptotic effects of a modern, n-6-reduced lipid emulsion mixture in murine ARDS. Methods: Mice received an infusion of either normal saline solution, pure long-chain triglyceride (LCT) emulsion, or SMOF (soybean oil, medium-chain triglycerides, olive oil, and fish oil) before a lipopolysaccharide (LPS) challenge. Mice were sacrificed at different time points (0, 24, or 72 h) after ARDS induction, and an analysis of inflammatory cytokines, protein concentrations, and the cellular composition of the alveolar and interstitial compartments was performed with special focus on alveolar apoptosis and necrosis. Results: Mice infused with SMOF showed decreased leukocyte invasion, protein leakage, myeloperoxidase activity, and cytokine production in alveolar spaces after LPS challenge compared to animals that received LCT. There were fewer cells in the lung interstitium of the SMOF group compared to the LCT group. Both lipid emulsions exerted pro-apoptotic and pro-necrotic properties on alveolar immune cells, with significantly increased necrosis in mice infused with LCT compared to SMOF. Conclusion: SMOF has both anti-inflammatory and pro-resolving influences in murine ARDS. Partial replacement of n-6 fatty acids with n-3/n-9 fatty acids may therefore benefit critically ill patients at risk for ARDS who require parenteral nutrition.
Role of Lopinavir/Ritonavir in the Treatment of Covid-19: A Review of Current Evidence, Guideline Recommendations, and Perspectives
Authors: Meini Simone , Pagotto Alberto , Longo Benedetta , Vendramin Igor , Pecori Davide , Tascini Carlo
Published: June 2020

A life-threatening respiratory illness (COVID-19) due to severe acute respiratory syndrome (SARS)-CoV-2 coronavirus was first described in December 2019 in Wuhan (China), rapidly evolving into a pandemic. In the first phase, when the viral replication plays a pivotal pathogenetic role, antiviral drugs could be crucial in limiting viral-induced organ damage. Unfortunately, there are no specific antivirals of proven efficacy for COVID-19, and several drugs have been repurposed to face this dramatic pandemic. In this paper we review the studies evaluating lopinavir/ritonavir association (LPV/r) use in COVID-19, and previously in SARS and Middle East respiratory syndrome (MERS). We searched PubMed to identify all relevant clinical and laboratory studies published up to 15 May 2020; the guidelines on the use of LPV/r in COVID-19 were further directly searched on the website of the main international scientific societies and agencies. Available evidence is currently scarce and of low quality. The recommendations issued for COVID-19 vary from positions clearly against the use of LPV/r to other positions that are more favorable. In our opinion, despite the controversial results of an important randomized clinical trial, and some recommendations, clinicians should not abandon the use of LPV/r for the treatment of COVID-19, possibly using this drug inside a prospective randomized trial, waiting for the results of the numerous ongoing trials evaluating its efficacy.
Systematic Kleihauer–Betke Test after External Cephalic Version for Breech Presentation: Is It Useful?
Authors: Lemaitre Johanna , Planche Lucie , Ducarme Guillaume
Published: June 2020

The incidence of fetomaternal hemorrhage (FMH) after external cephalic version (ECV) has been poorly reported. In this study, we evaluated the frequency of FMH, diagnosed by positive Kleihauer–Betke test (KBT), after ECV attempt and then evaluate the relevance of its routine use after procedure. A total of 282 women with a term breech presentation and who had ECV attempt were recruited from January 2014 and December 2018. After ECV attempt, women were systematically screened for FMH using KBT. Data on ECV attempt, KBT results, perinatal and neonatal outcomes were collected and compared between women with positive (cases) and negative KBT (controls) after ECV. The success rate of ECV was 22.0% (62/282). Eight women (2.9%) experienced transient fetal heart rate (FHR) abnormalities after ECV. In five (1.8%) women, KBT was positive after ECV. Obstetrical management was modified for two of these five women due to continuous positivity of KBT at day 1 and day 7 controls after ECV attempt. A cesarean section was planned 7 days earlier due to persistent high FMH on day 7 (6 mL fetal blood) in one woman and the labor was induced for persistent high FMH on day 7 (20 mL fetal blood) for another woman. No newborns have signs of fetal anemia at birth and there was no significant difference in neonatal status between two groups. FMH after ECV attempt are rare, and no negative fetal or neonatal outcomes were observed when KBT was positive, even strongly (>5 mL fetal blood). It appears that systematic KBT after attempted ECV is probably not useful.
Childhood Trauma Predicts Less Remission from PTSD among Patients with Co-Occurring Alcohol Use Disorder and PTSD
Authors: Brunault Paul , Lebigre Kevin , Idbrik Fatima , Maugé Damien , Adam Philippe , Barrault Servane , Baudin Grégoire , Courtois Robert , El Ayoubi Hussein , Grall-Bronnec Marie , Hingray Coraline , Ballon Nicolas , El-Hage Wissam
Published: June 2020

Post-traumatic stress disorder (PTSD) is highly prevalent among patients hospitalized for an alcohol use disorder (AUD). Hospitalization can improve PTSD and AUD outcomes in some but not all patients, but we lack data on the baseline predictors of PTSD non-remission. This study aimed to determine the baseline risk factors for non-remitted PTSD in patients hospitalized for an AUD. Of 298 AUD inpatients recruited in a rehabilitation center (Le Courbat, France), we included 91 AUD inpatients with a co-occurring PTSD and a longitudinal assessment at baseline (T1) and before discharge (T2: 8 weeks later). Patients were assessed for PTSD diagnosis/severity (PCL-5=PTSD Checklist for DSM-5), different types of trauma including childhood trauma (LEC-5=Life Events Checklist for DSM-5/CTQ-SF=Childhood Trauma Questionnaire, Short-Form), and AUD diagnosis/severity (clinical interview/AUDIT=Alcohol Use Disorders Identification Test). Rate of PTSD remission between T1 and T2 was 74.1%. Non-remitted PTSD at T2 was associated with a history of childhood trauma (physical, emotional or sexual abuse, physical negligence), but not with other types of trauma experienced, nor baseline PTSD or AUD severity. Among patients hospitalized for an AUD with co-occurring PTSD, PTSD remission was more strongly related to the existence of childhood trauma than to AUD or PTSD severity at admission. These patients should be systematically screened for childhood trauma in order to tailor evidence-based interventions.
Mild Cognitive Impairment Subtypes and Type 2 Diabetes in Elderly Subjects
Authors: Valenza Silvia , Paciaroni Lucia , Paolini Susy , Bonfigli Anna Rita , Di Rosa Mirko , Rabini Rosa Anna , Tortato Elena , Pelliccioni Paolo , Pelliccioni Giuseppe
Published: June 2020

Background: Type 2 diabetes (T2D) is correlated to amnestic mild cognitive impairment (aMCI) and to non-amnestic mild cognitive impairment (naMCI). This study evaluated whether the T2D variable characterizes a peculiar cognitive profile in elderly patients. Moreover, it explores the association between glycated hemoglobin levels (HbA1c), T2D duration, insulin and oral hypoglycemic agent treatment, and cognition in elderly diabetic patients. Methods: Detailed neuropsychological battery was used to diagnose MCI subtypes. A total of 39 MCI subjects with T2D (T2D-MCI) and 37 MCI subjects without T2D (ND-MCI), matched for age, educational level, and Mini-Mental State Examination score, were included. Results: ND-MCI performed worse in memory and language domains than T2D-MCI. The amnestic subtype is more frequent among ND-MCI and non-amnestic subtype in T2D-MCI. In T2D-MCI, high HbA1c levels correlate with episodic memory (immediate recall) and T2D duration. Some indexes of episodic memory (immediate recall), attention, and visual-spatial ability correlate with insulin treatment. Conclusions: An association between T2D and non-amnestic MCI is suggested. In the T2D-MCI group, significant associations between insulin treatment and memory (immediate recall), complex figure copy, and attention were found.
The Impact of Preoperative Risk on the Association between Hypotension and Mortality after Cardiac Surgery: An Observational Study
Authors: Ristovic Vanja , de Roock Sophie , Mesana Thierry G. , van Diepen Sean , Sun Louise Y.
Published: June 2020

Background: Despite steady improvements in cardiac surgery-related outcomes, our understanding of the physiologic mechanisms leading to perioperative mortality remains incomplete. Intraoperative hypotension is an important risk factor for mortality after noncardiac surgery but remains relatively unexplored in the context of cardiac surgery. We examined whether the association between intraoperative hypotension and in-hospital mortality varied by patient and procedure characteristics, as defined by the validated Cardiac Anesthesia Risk Evaluation (CARE) mortality risk score. Methods: We conducted a retrospective cohort study of consecutive adult patients who underwent cardiac surgery requiring cardiopulmonary bypass (CPB) from November 2009–March 2015. Those who underwent off-pump, thoracic aorta, transplant and ventricular assist device procedures were excluded. The primary outcome was in-hospital mortality. Hypotension was categorized by mean arterial pressure (MAP) of <55 and between 55–64 mmHg before, during and after CPB. The relationship between hypotension and death was modeled using multivariable logistic regression in the intermediate and high-risk groups. Results: Among 6627 included patients, 131 (2%) died in-hospital. In-hospital mortality in patients with CARE scores of 1, 2, 3, 4 and 5 was 0 (0%), 7 (0.3%), 35 (1.3%), 41 (4.6%) and 48 (13.6%), respectively. In the intermediate-risk group (CARE = 3–4), MAP < 65 mmHg post-CPB was associated with increased odds of death in a dose-dependent fashion (adjusted OR 1.30, 95% CI 1.13–1.49, per 10 min exposure to MAP < 55 mmHg, p = 0.002; adjusted OR 1.18 [1.07–1.30] per 10 min exposure to MAP 55–64 mmHg, p = 0.001). We did not observe an association between hypotension and mortality in the high-risk group (CARE = 5). Conclusions: Post-CPB hypotension is a potentially modifiable risk factor for mortality in intermediate-risk patients. Our findings provide impetus for clinical trials to determine if hemodynamic goal-directed therapies could improve survival in these patients.
Mesenchymal Stromal Cell-Based Therapy—An Alternative to Arthroplasty for the Treatment of Osteoarthritis? A State of the Art Review of Clinical Trials
Authors: Maleitzke Tazio , Elazaly Hisham , Festbaum Christian , Eder Christian , Karczewski Daniel , Perka Carsten , Duda Georg N. , Winkler Tobias
Published: June 2020

Osteoarthritis (OA) is the most common degenerative joint disorder worldwide and to date no regenerative treatment has been established in clinical practice. This review evaluates the current literature on the clinical translation of mesenchymal stromal cell (MSC)-based therapy in OA management with a focus on safety, outcomes and procedural specifics. PubMed, Cochrane Library and clinicaltrials.gov were searched for clinical studies using MSCs for OA treatment. 290 articles were initially identified and 42 articles of interest, including a total of 1325 patients, remained for further examination. Most of the included studies used adipose tissue-derived MSCs or bone-marrow-derived MSCs to treat patients suffering from knee OA. MSC-based therapy for knee OA appears to be safe and presumably effective in selected parameters. Yet, a direct comparison between studies was difficult due to a pronounced variance regarding methodology, assessed outcomes and evidence levels. Intensive scientific engagement is needed to identify the most effective source and dosage of MSCs for OA treatment in the future. Consent on outcome measures has to be reached and eventually patient sub-populations need to be identified that will profit most from MSC-based treatment for OA.
A Characterization of the Damage Process under Buckling Load in Composite Reinforced by Flax Fibres
Authors: Fehri Meriem , Vivet Alexandre , Dammak Fakhreddine , Haddar Mohamed , Keller Clément
Published: June 2020

The purpose of this work is to analyze the damage process resulting from buckling load applied on composites reinforced by flax fibre. Continous buckling test was performed on specimens until cracks appeared on their outer face. This test was monitored with an acoustic emission system. The high sensitivity of this method allows the detection of any process or mechanism generating sound waves. Moreover, this technic has the advantage of not causing contact in the deformed zone and thus to overcome the parasitic damage that may result from the stress concentrations in these areas. A multiparametric analysis is used to identify the acoustic signatures corresponding to each damage mechanism involved in the materials, and then follow their evolution in order to identify the most critical mechanisms leading to the final breakage of the material. The presence of these damage mechanisms was confirmed post-test by microscopic observations. Three orientations of laminate specimens (0°, 90° and 45°), relative to flax fabric architecture, were tested in order to characterize and highlight on their own damage process. Similarities as differences were observed between these mechanisms. We have deduced that the high porosity rate found in our composites are resulting from manufacturing parameters. Architecture and properties of the flax fabric influenced negatively the mechanical properties later by accentuating the gap between theoretical and practical values (17% to 22.4%) and by accelerating the development of certain damages such as matrix cracking which acoustic hit density is superior to 70% and fiber/matrix decohesion which occurs very early.
Intermittent Pneumatic Compression and Cold Water Immersion Effects on Physiological and Perceptual Recovery during Multi-Sports International Championship
Authors: Martínez-Guardado Ismael , Rojas-Valverde Daniel , Gutiérrez-Vargas Randall , Ugalde Ramírez Alexis , Gutiérrez-Vargas Juan Carlos , Sánchez-Ureña Braulio
Published: June 2020

Background: Congested-fixture championships are common during the selection of the athletes and teams participating in the Olympic Games. Throughout these tournaments, it is fundamental to perform optimally, rest well, and recover between competitions. This study aimed to (a) explore the effectiveness of the use of intermittent pneumatic compression (IPC) and cold water immersion (CWI) to recover muscle mechanical function (MuscleMechFx), hydration status (HydS), pain perception (PainPercep), rate of perceived exertion (RPE), sleep hours, and sleep quality (SleepQual) during a regional multi-sports international championship and (b) compare these results by sex. Methods: A total of 52 basketball and handball players were exposed to a recovery protocol after the competition as follows: IPC, sequential 20 min at 200 mmHg, and CWI, continuous 12 min at 12 °C. Results: MuscleMechFx presented differences by match and sex (p = 0.058) in time of contraction of biceps femoris; SleepQual and sleep hours were different between matches (<0.01). Conclusions: IPC + CWI seems to be effective to maintain some MuscleMechFx, HydS, and recovery and pain perception during a congested multi-sport tournament.
International Society for Human and Animal Mycology (ISHAM)—New Initiatives
Authors: Chakrabarti Arunaloke , Meis Jacques F. , Cornely Oliver A.
Published: June 2020

Fungal infections have emerged as major threat to human beings. The world is not ready to face this formidable challenge due to limited awareness, insufficient laboratories, and difficulty in managing mycoses especially in developing countries. The International Society for Human and Animal Mycology (ISHAM) has undertaken several new initiatives to overcome these gaps, including a global outreach program with national affiliated mycology societies and other regional groups. ISHAM is working closely with the European Confederation of Medical Mycology (ECMM) and Global Action Fund for Fungal Infections (GAFFI) to enhance these efforts. The society has launched laboratory e-courses and is in the process of the development of clinical e-courses. ISHAM has partnered with regional conferences in South America and Asia by sponsoring international experts and young delegates. The society also supports young people from less developed countries to undergo training in laboratories of excellence. ISHAM facilitated the formation of the INFOCUS-Latin American Clinical Mycology Working Group (LATAM) and the Pan-African Mycology Working Group. The society appointed country ambassadors to facilitate coordination with national societies. Still, the task is enormous and ISHAM calls for strong advocacy and more coordinated activities to attract the attention of people from all disciplines to this neglected field.
Quality Labels as Drivers of Peri-Urban Livestock Systems Resilience
Authors: Filippini Rosalia , Gennai-Schott Sabine , Sabbatini Tiziana , Lardon Sylvie , Marraccini Elisa
Published: June 2020

Livestock farming systems have an important role in the territorial systems of the Mediterranean, but in the last twenty years the sector has undergone serious changes with an important decrease in the number of farms. The purpose of this study is to show the contribution of a local food certification to the resilience of peri-urban livestock farming system and of its food supply chain at territorial level. The focus is on the “Carne Bovina di Pisa” project, a private label promoted by the local livestock producers’ association, with the purpose of preserving biodiversity and provide farmers with an opportunity to strengthen their local marketing power. The case study is the peri-urban area of Pisa (Tuscany, Italy), representative of the urbanized Mediterranean coastal plains with high urban pressure on agricultural land and increasing agricultural abandonment in the peri-urban area. The analysis is based on the qualitative analysis of interviews to stakeholders and the quantitative figures about the changes in livestock system. Results show that the label has positively sustained both the resilience of farming systems and the local food supply chains.
The Possibility of Applying Acoustic Emission and Dynamometric Methods for Monitoring the Turning Process
Authors: Dudzik Krzysztof , Labuda Wojciech
Published: June 2020

Ensuring optimal turning conditions has a huge impact on the quality and properties of the machined surface. The condition of the cutting tool is one of the factors to achieve this goal. In order to control its wear during the turning process, monitoring was used. In this study, the acoustic emission method and measure of cutting forces during turning were used for monitoring that process. The research was carried out on a universal lathe center (CU500MRD type) using a Kistler dynamometer with assembled removable insert CCET09T302R-MF by DIJET Industrial CO., LTD. A dynamometer allows to measure forces Fx (radial force), Fy (feed force) and Fz (cutting force). The turning process was performed on a shaft with 60 mm diameter made of 304L stainless steel. The AE research was carried at Physical Acoustics Corporation with the kit that includes: recorder USB AE Node, preamplifier, AE-sensor VS 150M and computer with dedicated software used for recording and analyzing AE data. The aim of this paper is to compare selected diagnostic methods: acoustic emission and cutting forces measurement for monitoring wear of cutting tool edge. Analysis of the research results showed that both selected methods of monitoring the turning process allowed the determination of the beginning of the tool damage process.
Experimental and Theoretical Study of the Covalent Grafting of Triazole Layer onto the Gold Surface
Authors: Orqusha Nimet , Phal Sereilakhena , Berisha Avni , Tesfalidet Solomon
Published: June 2020

Finding novel strategies for surface modification is of great interest in electrochemistry and material sciences. In this study, we present a strategy for modification of a gold electrode through covalent attachment of triazole (TA) groups. Triazole groups were electrochemically grafted at the surface of the electrode by a reduction of in situ generated triazolediazonium cations. The resulting grafted surface was characterized before and after the functionalization process by different electrochemical methods (cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS)) confirming the presence of the grafted layer. The grafting of TA on the electrode surface was confirmed using analysis of surface morphology (by atomic force microscopy), the thickness of the grafted layer (by ellipsometry) and its composition (by X-ray photoelectron spectroscopy). Density functional theory (DFT) calculations imply that the grafted triazole offers a stronger platform than the grafted aryl layers.
Autogenous Fiber Laser Welding of 316L Austenitic and 2304 Lean Duplex Stainless Steels
Authors: Landowski Michał , Świerczyńska Aleksandra , Rogalski Grzegorz , Fydrych Dariusz
Published: June 2020

This study presents results of experimental tests on quality of dissimilar welded joints between 316L austenitic and 2304 lean duplex stainless steels, welded without ceramic backing. Fiber laser welded butt joints at a thickness of 8 mm were subjected to non-destructive testing (visual and penetrant), destructive testing (static tensile test, bending test, and microhardness measurements) and structure observations (macro- and microscopic examinations, SEM, element distribution characteristics, and ferrite content measurements). Non-destructive tests and metallographic examinations showed that the welded joints meet the acceptance criteria for B level in accordance with EN ISO 13919–1 standard. Also the results of the destructive tests confirmed the high quality of the joints: specimens were fractured in base material with lower strength—316L austenitic stainless steel and a 180° bending angle was obtained confirming the high plasticity of the joints. Microscopic examination, SEM and EDS analysis showed the distribution of alloying elements in joints. The microhardness of the autogenous weld metal was higher by about 20 HV0.2 than that of the lean duplex steel. Ferrite content in the root was about 37% higher than in the face of the weld. The Schaeffler phase diagram was used to predict the phase composition of the welded joints and sufficient compliance with the magnetic method was found. The presented procedure can be used for welding of 316L–2304 stainless steels dissimilar welded joints of 8 mm thickness without ceramic backing.
Atmospheric Pressure Plasma Deposition of TiO2: A Review
Authors: Banerjee Soumya , Adhikari Ek , Sapkota Pitambar , Sebastian Amal , Ptasinska Sylwia
Published: June 2020

Atmospheric pressure plasma (APP) deposition techniques are useful today because of their simplicity and their time and cost savings, particularly for growth of oxide films. Among the oxide materials, titanium dioxide (TiO2) has a wide range of applications in electronics, solar cells, and photocatalysis, which has made it an extremely popular research topic for decades. Here, we provide an overview of non-thermal APP deposition techniques for TiO2 thin film, some historical background, and some very recent findings and developments. First, we define non-thermal plasma, and then we describe the advantages of APP deposition. In addition, we explain the importance of TiO2 and then describe briefly the three deposition techniques used to date. We also compare the structural, electronic, and optical properties of TiO2 films deposited by different APP methods. Lastly, we examine the status of current research related to the effects of such deposition parameters as plasma power, feed gas, bias voltage, gas flow rate, and substrate temperature on the deposition rate, crystal phase, and other film properties. The examples given cover the most common APP deposition techniques for TiO2 growth to understand their advantages for specific applications. In addition, we discuss the important challenges that APP deposition is facing in this rapidly growing field.
Photo-Mechanical Response Dynamics of Liquid Crystal Elastomer Linear Actuators
Authors: Grabowski Przemysław , Haberko Jakub , Wasylczyk Piotr
Published: June 2020

With continuous miniaturization of many technologies, robotics seems to be lagging behind. While the semiconductor technologies operate confidently at the nanometer scale and micro-mechanics of simple structures (MEMS) in micrometers, autonomous devices are struggling to break the centimeter barrier and have hardly colonized smaller scales. One way towards miniaturization of robots involves remotely powered, light-driven soft mechanisms based on photo-responsive materials, such as liquid crystal elastomers (LCEs). While several simple devices have been demonstrated with contracting, bending, twisting, or other, more complex LCE actuators, only their simple behavior in response to light has been studied. Here we characterize the photo-mechanical response of a linear light-driven LCE actuator by measuring its response to laser beams with varying power, pulse duration, pulse energy, and the energy spatial distribution. Light absorption decrease in the actuator over time is also measured. These results are at the foundation of further development of soft, light-driven miniature mechanisms and micro-robots.
Effect of Alkaline-Basic Electrolytes on the Capacitance Performance of Biomass-Derived Carbonaceous Materials
Authors: Karamanova Boryana , Stoyanova Antonia , Shipochka Maria , Veleva Svetlana , Stoyanova Radostina
Published: June 2020

The present work explores in detail the effect of alkaline-basic electrolytes on the capacitance performance of biomass-derived carbonaceous materials used as electrodes in symmetric supercapacitors. The proof-of-concept is demonstrated by two commercial carbon products (YP-50F and YP-80F, Kuraray Europe GmbH, Vantaa, Finland), obtained from coconuts. The capacitance performance of YP-50F and YP-80F was evaluated in three types of basic electrolytes: 6 M LiOH, 6 M NaOH and 6 M KOH. It was found that the capacitance performance of YP-50F improved in the following order: NaOH < LiOH < KOH; Meanwhile, for YP-80F, the order changes to LiOH < NaOH < KOH. After 1000 cycles, the cycling stability of both YP-50F and YP-80F increased in the order NaOH < LiOH < KOH. This order of performance improvement is determined by both the electrolyte conductivity and the interaction between the functional groups of carbonaceous materials and alkaline electrolytes. The reactivity of the functional groups was assessed by postmortem SEM/EDS and X-ray photoelectron spectroscopy (XPS) analyses of the electrodes after prolonged cycling.
Strategies for Improving Antimicrobial Properties of Stainless Steel
Authors: Resnik Matic , Benčina Metka , Levičnik Eva , Rawat Niharika , Iglič Aleš , Junkar Ita
Published: June 2020

In this review, strategies for improving the antimicrobial properties of stainless steel (SS) are presented. The main focus given is to present current strategies for surface modification of SS, which alter surface characteristics in terms of surface chemistry, topography and wettability/surface charge, without influencing the bulk attributes of the material. As SS exhibits excellent mechanical properties and satisfactory biocompatibility, it is one of the most frequently used materials in medical applications. It is widely used as a material for fabricating orthopedic prosthesis, cardiovascular stents/valves and recently also for three dimensional (3D) printing of custom made implants. Despite its good mechanical properties, SS lacks desired biofunctionality, which makes it prone to bacterial adhesion and biofilm formation. Due to increased resistance of bacteria to antibiotics, it is imperative to achieve antibacterial properties of implants. Thus, many different approaches were proposed and are discussed herein. Emphasis is given on novel approaches based on treatment with highly reactive plasma, which may alter SS topography, chemistry and wettability under appropriate treatment conditions. This review aims to present and critically discuss different approaches and propose novel possibilities for surface modification of SS by using highly reactive gaseous plasma in order to obtain a desired biological response.
Laminarin Attenuates Ultraviolet-Induced Skin Damage by Reducing Superoxide Anion Levels and Increasing Endogenous Antioxidants in the Dorsal Skin of Mice
Authors: Ahn Ji Hyeon , Kim Dae Won , Park Cheol Woo , Kim Bora , Sim Hyejin , Kim Hyun Sook , Lee Tae-Kyeong , Lee Jae-Chul , Yang Go Eun , Her Young , Park Joon Ha , Sim Tae Heung , Lee Hyun Sam , Won Moo-Ho
Published: June 2020

A number of studies have demonstrated that marine carbohydrates display anti-oxidant, anti-melanogenic, and anti-aging activities in the skin. Laminarin (LA), a low-molecular-weight polysaccharide, is found in brown algae. The benefits of LA in ultraviolet B (UVB) induced photodamage of the skin have not been reported. The aim of this study was to investigate the effects of pre-treated LA on histopathological changes and oxidative damage in mouse dorsal skin on day 5, following repeated UVB exposure. Histopathology, Western blot analysis and immunohistochemical studies showed that epidermal thickness in the UVB group was significantly increased; however, the thickness in the UVB group treated with LA (LA/UVB group) was less compared with that of the UVB group. Collagen fibers in the dermis of the UVB group were significantly decreased and destroyed, whereas, in the LA/UVB group, the density of collagen fibers was significantly increased compared with that of the UVB group. Oxidative stress due to superoxide anion production measured via dihydroethidium fluorescence staining was dramatically increased in the UVB group, whereas in the LA/UVB group, the oxidative stress was significantly decreased. Expressions of SOD1, glutathione peroxidase and catalase were markedly reduced in the UVB group, whereas in the LA/UVB group, they were significantly higher along with SOD2 than in the control group. Taken together, our results indicate that LA pretreatment prevents or attenuates skin damage, by decreasing oxidative stress and increasing antioxidant enzymes in mouse dorsal skin.
Non-Autogenous Innovative Reconstruction Method Following Mandibulectomy
Authors: Haj Yahya Bahaa , Rosenfeld Eli , Chaushu Gavriel , Kaplan Ilana , Ben-Zvi Yehonantan , Hamzani Yafit
Published: June 2020

Plexiform ameloblastoma is a locally aggressive odontogenic tumor, rare in the anterior mandible. The treatment of choice is resection with 1–3 cm free margins. In most of reported cases, the affected mandible is reconstructed by autogenic bone graft or osseocutaneous microvascular free flap in order to return function and esthetics. Case description: A 2 cm diameter exophytic ameloblastoma, located in the anterior mandible of a 50-year-old male was resected and reconstructed in a unique manner—allogenic bone block, recombinant human bone morphogenetic protein (rhBMP) and xenograft particles via transcutaneous submental approach. After bone maturation, dental implants were placed and restored by fixed prosthetics. Practical implications: Mandible reconstruction modalities have a crucial influence on patient quality of life, function and esthetics. Allogenic bone block combined with rhBMP and xenograft particles can replace the traditional autogenous bone in certain circumstances. A submental transcutaneous “tent pole” approach can improve the success rate of the reconstruction procedure.
The Effects of Tai Chi and Qigong on Immune Responses: A Systematic Review and Meta-Analysis
Authors: Oh Byeongsang , Bae Kyeore , Lamoury Gillian , Eade Thomas , Boyle Frances , Corless Brian , Clarke Stephen , Yeung Albert , Rosenthal David , Schapira Lidia , Back Michael
Published: June 2020

Background: Effective preventative health interventions are essential to maintain well-being among healthcare professionals and the public, especially during times of health crises. Several studies have suggested that Tai Chi and Qigong (TQ) have positive impacts on the immune system and its response to inflammation. The aim of this review is to evaluate the current evidence of the effects of TQ on these parameters. Methods: Electronic searches were conducted on databases (Medline, PubMed, Embase and ScienceDirect). Searches were performed using the following keywords: “Tai Chi or Qigong” and “immune system, immune function, immunity, Immun*, inflammation and cytokines”. Studies published as full-text randomized controlled trials (RCTs) in English were included. Estimates of change in the levels of immune cells and inflammatory biomarkers were pooled using a random-effects meta-analysis where randomised comparisons were available for TQ versus active controls and TQ versus non-active controls. Results: Nineteen RCTs were selected for review with a total of 1686 participants and a range of 32 to 252 participants within the studies. Overall, a random-effects meta-analysis found that, compared with control conditions, TQ has a significant small effect of increasing the levels of immune cells (SMD, 0.28; 95% CI, 0.13 to 0.43, p = 0.00), I2 = 45%, but not a significant effect on reducing the levels of inflammation (SMD, −0.15; 95% CI, −0.39 to 0.09, p = 0.21), I2 = 85%, as measured by the systemic inflammation biomarker C-reactive protein (CRP) and cell mediated biomarker cytokines. This difference in results is due to the bidirectional regulation of cytokines. An overall risk of bias assessment found three RCTs with a low risk of bias, six RCTs with some concerns of bias, and ten RCTs with a high risk of bias. Conclusions: Current evidence indicates that practising TQ has a physiologic impact on immune system functioning and inflammatory responses. Rigorous studies are needed to guide clinical guidelines and harness the power of TQ to promote health and wellbeing.
The Separation of Aluminum and Stainless-Steel Scraps Using Vibrating Mixed-Size Ball Bed
Authors: Na Hyunjin , Yoo Kyoungkeun , Jha Manis Kumar , Tabelin Carlito Baltazar
Published: June 2020

Dry gravity separation using a vibrating zirconia ball bed is proposed in this study to separate aluminum (Al) and stainless steel (STS) scraps obtained from spent hard disk drive recycling. The effects of zirconia ball sizes and vibrating power (vibration amplitude) on the separation efficiency of Al and STS scraps were investigated. The zirconia balls moved down at the center of the vessel and rose with the wall during the vibration test. Although more STS scraps sunk than Al scraps did, the separation efficiency was not maintained because Al scraps also sunk along with balls’ movement. The separation efficiency increased to 86.6% using 1-mm zirconia balls with a 2.5-mm vibration amplitude at 4 min, but it decreased rapidly by ball moving. Therefore, when a ball bed of mixed sizes (2:1 ratio of 1 and 3 mm) was used and arranged, whereby the 3-mm zirconia balls were above the 1-mm ball bed, the separation efficiency increased to 100% for more than 2 min. This dramatic improvement was because the 3-mm ball bed acted as a barrier to prevent sunken STS scraps from rising, and Al scrap cannot sink through the 3-mm ball bed. These results indicate that the separation of Al and STS scraps could be achieved successfully using the dry gravity separation method.