bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of Histological Techniques to Study the Reproductive Biology of the Hermaphroditic Mediterranean Fishes Coris julis, Serranus scriba, and Diplodus annularis
Authors: Alonso-Fernández Alexandre , Alós Josep , Grau Amalia , Domínguez-Petit Rosario , Saborido-Rey Fran
Published: July 2012

The usefulness and importance of histological techniques in reproductive studies have been widely discussed for gonochoristic fish species. In the case of hermaphroditic fishes, histology is a particularly important tool for the proper identification of sexual pattern (i.e., sequential, simultaneous, or nonfunctional hermaphroditism). We used a histological approach to describe hermaphroditism, dynamics of follicle development, and spawning patterns in three species from the northwestern Mediterranean Sea (Balearic Islands, Spain): the Mediterranean rainbow wrasse Coris julis (sequential hermaphrodite), painted comber Serranus scriba (simultaneous hermaphrodite), and annular sea bream Diplodus annularis (nonfunctional hermaphrodite). Development of secondary growth follicles was asynchronous in all three species, and similar peaks in spawning activity occurred between May and July. However, notable differences in sexual cycle and egg production were found. For the painted comber, hydrated follicles were present in ovarian tissue almost every day during the peak of the spawning season, suggesting daily spawning and increasing the chances of autofertilization unless a mechanism to avoid this action is present in this species. Intersexual Mediterranean rainbow wrasses were identified, and the size and age at sex change were estimated to be 132 mm total length and 4 years, respectively. Previous investigators have concluded that the annular sea bream is a protandric hermaphrodite, but our results indicate nonfunctional hermaphroditism. These three species are of little commercial interest and are considered to be bycatch by the artisanal fleet, but they are vulnerable to the impacts of some recreational angling activities. Currently, no specific management plan has been developed for these species. We address the importance of describing sexual pattern and its implications for future conservation efforts.
Seasonal, Small-Scale Distribution of Atka Mackerel in the Aleutian Islands, Alaska, with Respect to Reproduction
Authors: Cooper Daniel , McDermott Susanne
Published: July 2012

We investigated the spatial distribution of Atka mackerel Pleurogrammus monopterygius with respect to maturity stage at Seguam Pass in the Aleutian Islands as well as their spawning locations within trawlable areas at three small-scale (10–20-nautical mile or 18.5–37.0-km) sites. Histological methods were used to determine maturity stages, and male maturity stages are described for the first time. Spawning areas were identified by catch per unit effort of demersal egg masses. At Seguam Pass, spawning was concentrated in the area adjacent to the east side of Amlia Island and between Amlia and Seguam islands. The observed spatial segregation of Atka mackerel by sex and maturity stage appears to interact with the boundaries of the trawl exclusion zone (TEZ) at Seguam Pass. During spawning, mature fish aggregate inside the closed area and immature fish and nonreproductively active mature males aggregate outside the TEZ in the area open to fishing. This increased the commercial catch selectivity of nonreproductively active males and immature fish during the September fishery. The spawning areas observed at Seguam and Tanaga passes and near Amchitka Island were within TEZs, which may serve as de facto marine protected areas for spawning Atka mackerel.
Sampling Efficiency of Longlines for Shortraker and Rougheye Rockfish Using Observations from a Manned Submersible
Authors: Rodgveller Cara J. , Sigler Michael F. , Hanselman Dana H. , Ito Daniel H.
Published: July 2012

Populations of demersal rockfish of the genus Sebastes are challenging to assess because they inhabit rocky areas that are difficult to sample with trawl gear. In contrast, longline gear can sample rocky areas, but several factors besides fish density can affect the relationship between catch rates and density. In this study, longline catch rates of shortraker rockfish Sebastes borealis and rougheye rockfish S. aleutianus were compared with observations of density from a manned submersible to evaluate the species’ catchability on longline gear. On separate occasions, rockfish behavior in the presence of longline gear was observed from the submersible. Densities averaged 3.0 shortraker and rougheye rockfish (combined) per 330 m2 of bottom (the effectively sampled area of a 100-m transect). Longline catch rates averaged 2.7 shortraker and rougheye rockfish per skate of 45 hooks. Longline catch rates were not statistically affected by submersible observations. There was a positive trend between density and longline catch rates, but the relationship was not significant. As observed from the submersible, the proportion of fish free-swimming near the longline increased through the duration of the set, indicating that rockfish were attracted to the line faster than they were caught. The catching process for shortraker and rougheye rockfish lasts longer than for more mobile species such as sablefish Anoplopoma fimbria.
Contrasting Early Marine Ecology of Chinook Salmon and Coho Salmon in Southeast Alaska: Insight into Factors Affecting Marine Survival
Authors: Weitkamp L. A. , Orsi J. A. , Myers K. W. , Francis R. C.
Published: July 2012

To identify processes potentially contributing to the differential marine survival rates of Chinook salmon Oncorhynchus tshawytscha and coho salmon O. kisutch originating from Southeast Alaska, we compared the early marine ecology of the two species during the critical first summer in marine waters. We predicted that the higher survival rates for coho salmon relative to Chinook salmon were related to the larger size, faster growth, or different habitat or species associations of coho salmon. Our size and growth expectations were largely substantiated: juvenile coho salmon were larger than juvenile Chinook salmon and had faster length-based growth, although weight-based growth rates were similar. The most obvious difference was in their distributions. Juvenile coho salmon overlapped spatially and temporally with abundant juvenile pink salmon O. gorbuscha and chum salmon O. keta, whereas juvenile Chinook salmon were geographically separated from other salmonids. This suggests that coho salmon benefited from a predation buffer that did not extend to Chinook salmon. Our results indicate that factors influencing marine survival of juvenile Chinook salmon and coho salmon in Southeast Alaska are attributable to species-specific differences in their early marine distribution patterns and species interactions.
Temperature Effects on Bioelectrical Impedance Analysis (BIA) Used to Estimate Dry Weight as a Condition Proxy in Coastal Bluefish
Authors: Hartman Kyle J. , Phelan Beth A. , Rosendale John E.
Published: July 2012

The highly migratory nature of bluefish Pomatomus saltatrix makes comprehensive study of their populations and their potential responses to factors such as competition, habitat degradation, and climate change difficult. Body composition is an important ecological reference point for fish; however, estimating body composition in fish has been limited by analytical and logistical costs. We applied bioelectrical impedance analysis (BIA) to estimate one body composition component (percent dry weight) as a proxy of condition in bluefish. We used a tetra polar Quantum II BIA analyzer and measured electrical properties in the muscles of bluefish at two locations per fish (dorsal and ventral). In total, 96 bluefish ranging from 193 to 875 mm total length were used in model development and testing. On 59 of these fish BIA measures were taken at both 15°C and 27°C. Temperature had a significant negative effect on resistance and reactance. A subsample of these fish was then analyzed for dry weight as a percentage of their whole body weight (PDW), which is a good indicator of condition because it is highly correlated with fat content in fish. The BIA models predicting PDW inclusive of all lengths of bluefish were highly predictive for 15°C (stepwise regression) and 27°C. Regression (R2pred) values that estimate future predictive power suggest that both models were robust. Strong relationships between PDW and other body composition components, coupled with the BIA models presented here, provide the tools needed to quantitatively assess bluefish body composition across spatial and temporal scales for which assessment was previously impossible.
Marine Habitat Use by Anadromous Bull Trout from the Skagit River, Washington
Authors: Hayes Michael C. , Rubin Stephen P. , Reisenbichler Reginald R. , Goetz Fred A. , Jeanes Eric , McBride Aundrea
Published: July 2012

Acoustic telemetry was used to describe fish positions and marine habitat use by tagged bull trout Salvelinus confluentus from the Skagit River, Washington. In March and April 2006, 20 fish were captured and tagged in the lower Skagit River, while 15 fish from the Swinomish Channel were tagged during May and June. Sixteen fish tagged in 2004 and 2005 were also detected during the study. Fish entered Skagit Bay from March to May and returned to the river from May to August. The saltwater residency for the 13 fish detected during the out-migration and return migration ranged from 36 to 133 d (mean ± SD, 75 ± 22 d). Most bull trout were detected less than 14 km (8.5 ± 4.4 km) from the Skagit River, and several bay residents used the Swinomish Channel while migrating. The bull trout detected in the bay were associated with the shoreline (distance from shore, 0.32 ± 0.27 km) and occupied shallow-water habitats (mean water column depth, <4.0 m). The modified-minimum convex polygons (MMCPs) used to describe the habitats used by 14 bay fish showed that most areas were less than 1,000 ha. The mean length of the shoreline bordering the MMCPs was 2.8 km (range, 0.01–5.7 km) for bay fish and 0.6 km for 2 channel residents. Coastal deposits, low banks, and sediment bluffs were common shoreline classes found within the MMCPs of bay fish, while modified shoreline classes usually included concrete bulkheads and riprap. Mixed fines, mixed coarse sediments, and sand were common substrate classes found within MMCPs; green algae and eelgrass (Zostera sp.) vegetation classes made up more than 70% of the area used by bull trout. Our results will help managers identify specific nearshore areas that may require further protection to sustain the unique anadromous life history of bull trout.

Received October 25, 2010; accepted May 5, 2011
Dynamic remodeling of scaffold interactions in dendritic spines controls synaptic excitability
Authors: Moutin Enora , Raynaud Fabrice , Roger Jonathan , Pellegrino Emilie , Homburger Vincent , Bertaso Federica , Ollendorff Vincent , Bockaert Joël , Fagni Laurent , Perroy Julie
Published: July 2012

Scaffolding proteins interact with membrane receptors to control signaling pathways and cellular functions. However, the dynamics and specific roles of interactions between different components of scaffold complexes are poorly understood because of the dearth of methods available to monitor binding interactions. Using a unique combination of single-cell bioluminescence resonance energy transfer imaging in living neurons and electrophysiological recordings, in this paper, we depict the role of glutamate receptor scaffold complex remodeling in space and time to control synaptic transmission. Despite a broad colocalization of the proteins in neurons, we show that spine-confined assembly/disassembly of this scaffold complex, physiologically triggered by sustained activation of synaptic NMDA (N-methyl-d-aspartate) receptors, induces physical association between ionotropic (NMDA) and metabotropic (mGlu5a) synaptic glutamate receptors. This physical interaction results in an mGlu5a receptor–mediated inhibition of NMDA currents, providing an activity-dependent negative feedback loop on NMDA receptor activity. Such protein scaffold remodeling represents a form of homeostatic control of synaptic excitability.
PDZ interactions regulate rapid turnover of the scaffolding protein EBP50 in microvilli
Authors: Garbett Damien , Bretscher Anthony
Published: July 2012

Scaffolding proteins containing PDZ (postsynaptic density 95/discs large/zonula occludens-1) domains are believed to provide relatively stable linkages between components of macromolecular complexes and in some cases to bridge to the actin cytoskeleton. The microvillar scaffolding protein EBP50 (ERM-binding phosphoprotein of 50 kD), consisting of two PDZ domains and an ezrin-binding site, retains specific proteins in microvilli and is necessary for microvillar biogenesis. Our analysis of the dynamics of microvillar proteins in vivo indicated that ezrin and microvillar membrane proteins had dynamics consistent with actin treadmilling and microvillar lifetimes. However, EBP50 was highly dynamic, turning over within seconds. EBP50 turnover was reduced by mutations that inactivate its PDZ domains and was enhanced by protein kinase C phosphorylation. Using a novel in vitro photoactivation fluorescence assay, the EBP50–ezrin interaction was shown to have a slow off-rate that was dramatically enhanced in a PDZ-regulated manner by addition of cell extract to near in vivo levels. Thus, the linking of relatively stable microvillar components can be mediated by surprisingly dynamic EBP50, a finding that may have important ramifications for other scaffolding proteins.
Interleukin-1α controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33
Authors: Willart Monique A.M. , Deswarte Kim , Pouliot Philippe , Braun Harald , Beyaert Rudi , Lambrecht Bart N. , Hammad Hamida
Published: July 2012

House dust mite (HDM) is one of the most common allergens worldwide. In this study, we have addressed the involvement of IL-1 in the interaction between HDM and the innate immune response driven by lung epithelial cells (ECs) and dendritic cells (DCs) that leads to asthma. Mice lacking IL-1R on radioresistant cells, but not hematopoietic cells, failed to mount a Th2 immune response and did not develop asthma to HDM. Experiments performed in vivo and in isolated air–liquid interface cultures of bronchial ECs showed that TLR4 signals induced the release of IL-1α, which then acted in an autocrine manner to trigger the release of DC-attracting chemokines, GM-CSF, and IL-33. Consequently, allergic sensitization to HDM was abolished in vivo when IL-1α, GM-CSF, or IL-33 was neutralized. Thymic stromal lymphopoietin (TSLP) became important only when high doses of allergen were administered. These findings put IL-1α upstream in the cytokine cascade leading to epithelial and DC activation in response to inhaled HDM allergen.
No evidence for inositol 1,4,5-trisphosphate–dependent Ca2+ release in isolated fibers of adult mouse skeletal muscle
Authors: Blaauw Bert , del Piccolo Paola , Rodriguez Laura , Hernandez Gonzalez Victor-Hugo , Agatea Lisa , Solagna Francesca , Mammano Fabio , Pozzan Tullio , Schiaffino Stefano
Published: July 2012

The presence and role of functional inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs) in adult skeletal muscle are controversial. The current consensus is that, in adult striated muscle, the relative amount of IP3Rs is too low and the kinetics of Ca2+ release from IP3R is too slow compared with ryanodine receptors to contribute to the Ca2+ transient during excitation–contraction coupling. However, it has been suggested that IP3-dependent Ca2+ release may be involved in signaling cascades leading to regulation of muscle gene expression. We have reinvestigated IP3-dependent Ca2+ release in isolated flexor digitorum brevis (FDB) muscle fibers from adult mice. Although Ca2+ transients were readily induced in cultured C2C12 muscle cells by (a) UTP stimulation, (b) direct injection of IP3, or (c) photolysis of membrane-permeant caged IP3, no statistically significant change in calcium signal was detected in adult FDB fibers. We conclude that the IP3–IP3R system does not appear to affect global calcium levels in adult mouse skeletal muscle.
Engineered interaction between SUR1 and Kir6.2 that enhances ATP sensitivity in KATP channels
Authors: Pratt Emily B. , Zhou Qing , Gay Joel W. , Shyng Show-Ling
Published: July 2012

The ATP-sensitive potassium (KATP) channel consisting of the inward rectifier Kir6.2 and SUR1 (sulfonylurea receptor 1) couples cell metabolism to membrane excitability and regulates insulin secretion. Inhibition by intracellular ATP is a hallmark feature of the channel. ATP sensitivity is conferred by Kir6.2 but enhanced by SUR1. The mechanism by which SUR1 increases channel ATP sensitivity is not understood. In this study, we report molecular interactions between SUR1 and Kir6.2 that markedly alter channel ATP sensitivity. Channels bearing an E203K mutation in SUR1 and a Q52E in Kir6.2 exhibit ATP sensitivity ∼100-fold higher than wild-type channels. Cross-linking of E203C in SUR1 and Q52C in Kir6.2 locks the channel in a closed state and is reversible by reducing agents, demonstrating close proximity of the two residues. Our results reveal that ATP sensitivity in KATP channels is a dynamic parameter dictated by interactions between SUR1 and Kir6.2.
Measuring mating competition correctly: available evidence supports operational sex ratio theory
Authors: de Jong Karen , Forsgren Elisabet , Sandvik Hanno , Amundsen Trond
Published: July 2012

Central to sexual selection theory is the question of when individuals should compete for mates. Theory predicts that the sex ratio of ready-to-mate individuals (operational sex ratio; OSR) affects male and female mating competition. In accordance with this, the strength of mating competition, measured by agonistic behaviors and courtship displays, has been found to co-vary with the OSR in field populations of several species. However, laboratory experiments have often produced results that seemingly contradict OSR theory, especially for courtship behavior. We argue that this may be because experiments typically measure frequencies of competitive behaviors. Frequencies of courtship and agonistic behavior are not only affected by the level of mating competition, but also by the number of potential mates or competitors encountered. In contrast, the propensity to behave competitively at a given encounter represents a behavioral response, and thus directly reflects mating competition. We show in 2 simple models that 1) courtship frequency can be expected to respond differently from courtship propensity to changes in OSR and 2) an increase in frequency of agonistic behaviors could occur even if the propensity is not affected by the OSR. In a meta-analysis of studies on courtship competition, we show that frequency measures produced largely opposite results to propensity measures, as predicted by our model. Moreover, courtship propensity increased when the OSR became more biased toward competitors. This presents strong evidence that the OSR affects competition, in the form of courtship, as predicted by OSR theory.
PEEK Versus Ti Interbody Fusion Devices
Authors: Pelletier Matthew Henry , Cordaro Nicholas , Punjabi Vaibhav M. , Waites Matthew , Lau Abe , Walsh William R.
Published: July 2012

Study Design:

Comparative evaluation of in vitro and in vivo biomechanics, resulting fusion and histomorphometric aspects of polyetheretherketone (PEEK) versus titanium (Ti) interbody fusion devices in an animal model with similar volumes of bone graft.

Objective:

Identify differences in the characteristics of fusion and biomechanics immediately following implantation (time 0) and at 26 weeks with each interbody implant.

Summary of Background Data:

PEEK has been well accepted in spinal surgery, it provides a closer match to the mechanical properties of bone than metallic implants such as Ti. This is thought to reduce graft stress shielding and subsidence of interbody fusion devices. There remains controversy as to the overall influence of this as a factor influencing resultant fusion and initial stability. Although material modulus is 1 factor of importance, other design factors are likely to play a large role determining overall performance of an interbody implant.

Methods:

A Ti and PEEK device of similar size with a central void to accommodate graft material were compared. The PEEK device had a ridged surface on the caudal and cephalad surfaces, whereas Ti device allowed axial compliance and had bone ingrowth endplates and polished internal surfaces. A 2-level ALIF was performed in 9 sheep and fusion, biomechanics, and bone apposition were evaluated at 26 weeks. Time 0 in vitro biomechanical tests were performed to establish initial stability immediately after implantation.

Results:

No differences were detected in the biomechanical measures of each of the devices in in vitro time 0 tests. All levels were fused by 26 weeks with considerably lower range of motion when compared with in vitro tests. Range of motion in all modes of bending was reduced by over 70% when compared with intact values for axial rotation (Ti-74%, PEEK-71%), lateral bending (Ti-90%, PEEK-88%), and flexion/extension (Ti-92%, PEEK-91%). Mechanical properties of fusions formed with each implant did not differ; however, bone apposition was variable with polished internal Ti surfaces being lower than PEEK and treated Ti endplates showing the greatest levels. Graft material displayed axial trabecular alignment with both implants.

Conclusions:

Although material properties and surface characteristics resulted in differing amounts of biological integration from the host, both implants were capable of producing excellent fusion results using similar volumes of bone graft.
Gadd45b knockout mice exhibit selective deficits in hippocampus-dependent long-term memory
Authors: Leach Prescott T. , Poplawski Shane G. , Kenney Justin W. , Hoffman Barbara , Liebermann Dan A. , Abel Ted , Gould Thomas J.
Published: July 2012

Growth arrest and DNA damage-inducible β (Gadd45b) has been shown to be involved in DNA demethylation and may be important for cognitive processes. Gadd45b is abnormally expressed in subjects with autism and psychosis, two disorders associated with cognitive deficits. Furthermore, several high-throughput screens have identified Gadd45b as a candidate plasticity-related gene. However, a direct demonstration of a link between Gadd45b and memory has not been established. The current studies first determined whether expression of the Gadd45 family of genes was affected by contextual fear conditioning. Gadd45b, and to a lesser extent Gadd45g, were up-regulated in the hippocampus following contextual fear conditioning, whereas Gadd45a was not. Next, Gadd45b knockout mice were tested for contextual and cued fear conditioning. Gadd45b knockout mice exhibited a significant deficit in long-term contextual fear conditioning; however, they displayed normal levels of short-term contextual fear conditioning. No differences between Gadd45b knockout and wild-type mice were observed in cued fear conditioning. Because cued fear conditioning is hippocampus independent, while contextual fear conditioning is hippocampus dependent, the current studies suggest that Gadd45b may be important for long-term hippocampus-dependent memory storage. Therefore, Gadd45b may be a novel therapeutic target for the cognitive deficits associated with many neurodevelopmental, neurological, and psychiatric disorders.
Anterior cingulate cortex in schema assimilation and expression
Authors: Wang Szu-Han , Tse Dorothy , Morris Richard G.M.
Published: July 2012

In humans and in animals, mental schemas can store information within an associative framework that enables rapid and efficient assimilation of new information. Using a hippocampal-dependent paired-associate task, we now report that the anterior cingulate cortex is part of a neocortical network of schema storage with NMDA receptor-mediated transmission critical for information updating, and AMPA receptor-mediated transmission required for the expression and updating of stored information.
A Mathematical Model for BRASSINOSTEROID INSENSITIVE1-Mediated Signaling in Root Growth and Hypocotyl Elongation
Authors: van Esse G. Wilma , van Mourik Simon , Stigter Hans , ten Hove Colette A. , Molenaar Jaap , de Vries Sacco C.
Published: July 2012

Brassinosteroid (BR) signaling is essential for plant growth and development. In Arabidopsis (Arabidopsis thaliana), BRs are perceived by the BRASSINOSTEROID INSENSITIVE1 (BRI1) receptor. Root growth and hypocotyl elongation are convenient downstream physiological outputs of BR signaling. A computational approach was employed to predict root growth solely on the basis of BRI1 receptor activity. The developed mathematical model predicts that during normal root growth, few receptors are occupied with ligand. The model faithfully predicts root growth, as observed in bri1 loss-of-function mutants. For roots, it incorporates one stimulatory and two inhibitory modules, while for hypocotyls, a single inhibitory module is sufficient. Root growth as observed when BRI1 is overexpressed can only be predicted assuming that a decrease occurred in the BRI1 half-maximum response values. Root growth appears highly sensitive to variation in BR concentration and much less to reduction in BRI1 receptor level, suggesting that regulation occurs primarily by ligand availability and biochemical activity.
Cocrystals of 5-fluorocytosine. I. Coformers with fixed hydrogen-bonding sites
Authors: Tutughamiarso Maya , Wagner Guido , Egert Ernst
Published: July 2012

The antifungal drug 5-fluorocytosine (4-amino-5-fluoro-1,2-dihydropyrimidin-2-one) was cocrystallized with five complementary compounds in order to better understand its drug–receptor interaction. The first two compounds, 2-aminopyrimidine (2-amino-1,3-diazine) and N-acetylcreatinine (N-acetyl-2-amino-1-methyl-5H-imidazol-4-one), exhibit donor–acceptor sites for R
2
2(8) heterodimer formation with 5-fluorocytosine. Such a heterodimer is observed in the cocrystal with 2-aminopyrimidine (I); in contrast, 5-fluorocytosine and N-acetylcreatinine [which forms homodimers in its crystal structure (II)] are connected only by a single hydrogen bond in (III). The other three compounds 6-aminouracil (6-amino-2,4-pyrimidinediol), 6-aminoisocytosine (2,6-diamino-3H-pyrimidin-4-one) and acyclovir [acycloguanosine or 2-amino-9-[(2-hydroxyethoxy)methyl]-1,9-dihydro-6H-purin-6-one] possess donor–donor–acceptor sites; therefore, they can interact with 5-fluorocytosine to form a heterodimer linked by three hydrogen bonds. In the cocrystals with 6-aminoisocytosine (Va)–(Vd), as well as in the cocrystal with the antiviral drug acyclovir (VII), the desired heterodimers are observed. However, they are not formed in the cocrystal with 6-aminouracil (IV), where the components are connected by two hydrogen bonds. In addition, a solvent-free structure of acyclovir (VI) was obtained. A comparison of the calculated energies released during dimer formation helped to rationalize the preference for hydrogen-bonding interactions in the various cocrystal structures.
A time-resolved powder diffraction study of in-situ photodimerization kinetics of 9-methylanthracene using a CCD area detector and parametric Rietveld refinement
Authors: Mabied Ahmed F. , Müller Melanie , Dinnebier Robert E. , Nozawa Shunsuke , Hoshino Manabu , Tomita Ayana , Sato Tokushi , Adachi Shin-ichi
Published: July 2012

The [4π + 4π] photodimerization process of the 9-substituted anthracene derivative crystalline 9-methylanthracene (9-MA) was investigated using time-resolved X-ray powder diffraction. The study was carried out in-situ using a CCD area detector. Sequential and parametric Rietveld refinement was applied for quantitative phase analysis. The results of traditional sequential Rietveld refinement showed that the evolution of the dimerization process can be described using the Johnson–Mehl–Avrami–Kolmogorov (JMAK) model. The parameters of the JMAK equation were obtained successfully by parametric Rietveld refinement and suggest that the reaction follows heterogeneous nucleation and one-dimensional growth with a decreasing nucleation rate.
The natural tiling approach to cation conductivity in KAlO2 polymorphs
Authors: Voronin Vladimir I. , Shekhtman Georgi Sh. , Blatov Vladislav A.
Published: July 2012

A detailed analysis of correlations between structural features and cation conductivity is performed for KAlO2 polymorphs in a wide temperature range of 300–1023 K. To explore the migration maps of K+ cations we have used neutron diffraction data for low- and high-temperature KAlO2 polymorphs and applied for the first time a novel algorithm based on the natural tiling concept and implemented it into the program package TOPOS. Five independent elementary channels for the K+ cation migration have been revealed whose cross-sections were found to be essentially different in the low-temperature form, indicating a high anisotropy of the cation conductivity. During the transition to the cubic high-temperature phase all five channels become equivalent with sharply increased cross-sections that account for the jump-like increase of the cation conductivity and gives rise to its three-dimensional character.
Synthesis, structure and spectroscopic properties of a novel compound bis(benzylamino)silver(I) benzylcarbamate
Authors: Liu Jianguo , Zeng Xiaoyan , Li Hua
Published: July 2012

A novel silver-containing compound, bis(benzylamino)silver(I) benzylcarbamate, with an unusual molecular structure is easily synthesized by the reaction of benzylammonium benzylcarbamate and silver oxide. It crystallizes in the triclinic crystal system with the space group P\bar 1 with a = 5.2006 (5), b = 14.6298 (15), c = 14.7246 (15) Å, α = 68.729 (2), β = 83.507 (2), γ = 85.412 (2)° and Z = 2. In the crystal, one Ag atom coordinates with the two amino groups in two benzylamine molecules, and there are no silver–silver and silver–oxygen interactions. The carboxylate groups take part in balancing the electric charge and forming hydrogen bonds. Both the compound and the starting material benzylammonium benzylcarbamate exhibit room-temperature solid-state emissions with the peaks at 300 and 406 nm, respectively.
Comparative ultrastructural studies of the alterations to mouse lung parenchyma during Trichinella spiralis or Toxocara canis infection
Authors: DABROWSKA J. , WALSKI M. , DYBICZ M. , DOLIGALSKA M.
Published: July 2012

Trichinella spiralis and Toxocara canis larvae migrated through the lung and induced many alterations in the lung parenchyma. Using electron microscopy, we identified and described the histopathological changes. These changes resulted from mechanical damage or from local inflammatory reactions provoked by larvae. The pattern of changes was described between 6 and 12 days post‐infection (DPI) with T. spiralis larvae, and between 21 and 28 DPI with T. canis. The ultrastructural studies demonstrated that T. spiralis larvae migrating through the lungs evoked mainly destruction of type I epithelial cells, destruction of lamellar bodies of epithelial cells or extracellular alveolar lining layer. The severity of these changes was dependent on the number of infective larvae (400 or 800 T. spiralis larvae) and possibly the result of mechanical damage in the lung parenchyma. In contrast, infection with T. canis larvae initiated mainly eosinophilic perivasculitis and vasculitis as well as macrophage accumulation in the lung, which were additionally impacted by numerous crystalloid inclusions in macrophages. Trichinella spiralis larvae and T. canis larvae induced different pathological changes in the lungs of infected mice.
Comparative microscopic and molecular analysis of Thatcher near‐isogenic lines with wheat leaf rust resistance genes Lr2a, Lr3, LrB or Lr9 upon challenge with different Puccinia triticina races
Authors: Wang X. , McCallum B. D. , Fetch T. , Bakkeren G. , Marais G. F. , Saville B. J.
Published: July 2012

Thatcher near‐isogenic lines (NILs) of wheat carrying resistance gene Lr2a, Lr3, LrB or Lr9 were inoculated with Puccinia triticina races of virulence phenotype BBBD, MBDS, SBDG and FBDJ. Puccinia triticina infection structures were analysed under the fluorescence microscope over a course of 14 days after inoculation (dai). The relative proportion of P. triticina and wheat genomic DNA in infected leaves was estimated with a semiquantitative multiplex PCR analysis using P. triticina‐ and wheat‐specific primers. The occurrence of a hypersensitive response (HR), cellular lignification and callose deposition in inoculated plants was investigated microscopically. In interactions producing highly resistant infection type (IT) ‘0;’, a maximum of two haustorial mother cells per infection site were produced, and there was no increase in the proportion of P.  triticina genomic DNA in infected leaves, indicating the absence of P. triticina growth. In comparison, sizes of P. triticina colonies increased gradually in interactions producing moderately resistant IT ‘1’ and ‘2’, with the highest proportion of P. triticina genomic DNA found in leaves sampled at 14 dai. In interactions producing susceptible IT ‘3–4’, the highest proportion of P. triticina genomic DNA was found in leaves sampled at 10 dai (45·5–51·5%). HR and cellular lignification were induced in interactions producing IT ‘0;’ and ‘1’ at 1 dai but they were not observed in interactions producing IT ‘2’ until 2 dai. No HR or cellular lignification were induced in interactions producing susceptible IT ‘3–4’. Furthermore, a strong deposition of callose was induced in Lr9 + BBBD and Lr9 + FBDJ (IT ‘0;’), whereas this defence response was not induced in resistant or susceptible interactions involving Lr2a, Lr3 or LrB, indicating that Lr9 mediated resistance was different from that conditioned by Lr2a, Lr3 or LrB.
Neuroprotective effects of phenolic antioxidant tBHQ associate with inhibition of FoxO3a nuclear translocation and activity
Authors: Bahia Parmvir K. , Pugh Victoria , Hoyland Kimberley , Hensley Victoria , Rattray Marcus , Williams Robert J.
Published: July 2012

The Forkhead transcription factor, FoxO3a induces genomic death responses in neurones following translocation from the cytosol to the nucleus. Nuclear translocation of FoxO3a is triggered by trophic factor withdrawal, oxidative stress and the stimulation of extrasynaptic NMDA receptors. Receptor activation of phosphatidylinositol 3‐kinase (PI3K)–Akt signalling pathways retains FoxO3a in the cytoplasm, thereby inhibiting the transcriptional activation of death‐promoting genes. We hypothesized that phenolic antioxidants such as tert‐Butylhydroquinone (tBHQ), which is known to stimulate PI3K–Akt signalling, would inhibit FoxO3a translocation and activity. Treatment of cultured cortical neurones with NMDA increased the nuclear localization of FoxO3a, reduced the phosphorylation of FoxO3a, increased caspase activity and up‐regulated Fas ligand expression. In contrast the phenolic antioxidant, tBHQ, caused retention of FoxO3a in the cytosol coincident with enhanced PI3K‐ dependent phosphorylation of FoxO3a. tBHQ‐induced nuclear exclusion of FoxO3a was associated with reduced FoxO‐mediated transcriptional activity. Exposure of neurones to tBHQ inhibited NMDA‐induced nuclear translocation of FoxO3a, prevented NMDA‐induced up‐regulation of FoxO‐mediated transcriptional activity, blocked caspase activation and protected neurones from NMDA‐induced excitotoxic death. Collectively, these data suggest that phenolic antioxidants such as tBHQ oppose stress‐induced activation of FoxO3a and therefore have potential neuroprotective utility in neurodegeneration.
SnoN activates p53 directly to regulate aging and tumorigenesis
Authors: Pan Deng , Zhu Qingwei , Conboy Michael J. , Conboy Irina M. , Luo Kunxin
Published: July 2012

We have identified SnoN as a direct activator of p53 to accelerate aging and inhibit tumorigenesis. SnoN has been shown previously to promote proliferation and transformation by antagonizing TGFβ signaling. We show that elimination of this TGFβ antagonistic activity of SnoN in vivo results in accelerated aging and resistance to tumorigenesis. The SnoN knockin mice display a shortened lifespan, decreased reproductivity, osteoporosis, reduced regenerative capacity, and other aging phenotypes, similar to that found in mice expressing an active p53. These activities of SnoN rely on the ability of SnoN to activate p53. SnoN can bind directly to p53 and compete with Mdm2 for binding to p53, preventing p53 ubiquitination and degradation and additionally facilitating p53 acetylation and phosphorylation. SnoN also binds to p53 on the promoter of p53 responsive genes to promote transcription activation. This activation of p53 by SnoN is necessary for its antitumorigenic and progeria activities in vivo because elimination of one copy of p53 reverses the aging phenotypes and accelerates tumorigenesis. Thus, we have revealed a novel function of SnoN in regulating aging and tumorigenesis by directly activating p53.
Poverty and Support for Militant Politics: Evidence from Pakistan
Authors: Blair Graeme , Christine Fair C. , Malhotra Neil , Shapiro Jacob N.
Published: July 2012

Policy debates on strategies to end extremist violence frequently cite poverty as a root cause of support for the perpetrating groups. There is little evidence to support this contention, particularly in the Pakistani case. Pakistan's urban poor are more exposed to the negative externalities of militant violence and may in fact be less supportive of the groups. To test these hypotheses we conducted a 6,000‐person, nationally representative survey of Pakistanis that measured affect toward four militant organizations. By applying a novel measurement strategy, we mitigate the item nonresponse and social desirability biases that plagued previous studies due to the sensitive nature of militancy. Contrary to expectations, poor Pakistanis dislike militants more than middle‐class citizens. This dislike is strongest among the urban poor, particularly those in violent districts, suggesting that exposure to terrorist attacks reduces support for militants. Long‐standing arguments tying support for violent organizations to income may require substantial revision.
Validation of the Vancouver Chest Pain Rule: A Prospective Cohort Study
Authors: Jalili Mohammad , Hejripour Zia , Honarmand Amir Reza , Pourtabatabaei Nasim
Published: July 2012

ACADEMIC EMERGENCY MEDICINE 2012; 19:837–842 © 2012 by the Society for Academic Emergency Medicine

Objectives:  The objective was to validate the Vancouver Chest Pain Rule in an emergency department (ED) setting to identify very‐low‐risk patients with acute chest pain.

Methods:  A prospective cohort study was conducted on consecutive patients 25 years of age and older presenting to the ED with a chief complaint of acute chest pain during January 2009 to July 2009. According to the Vancouver Chest Pain Rule, cardiac history, chest pain characteristics, physical and electrocardiogram (ECG) findings, and cardiac biomarker measurement (creatine kinase‐myocardial band isoenzyme [CK‐MB]) were used to identify patients with very low risk for developing acute coronary syndrome (ACS) in 30 days. The primary outcome was defined as developing ACS (myocardial infarction or non‐ST‐elevation myocardial infarction [MI]/unstable angina) within 30 days of ED presentation, and all diagnoses were made using predefined explicit criteria. Sensitivity, specificity, positive predictive value, and negative predictive value were calculated.

Results:  Of 593 patients who were eligible for evaluation, 39 (6.6%) developed MI and 43 (7.3%) developed unstable angina. Among all patients, 292 (49.2%) patients could have been assigned to the very‐low‐risk group and discharged after a brief ED assessment according to the Vancouver Chest Pain Rule. Among these patients, four (1.4%) developed ACS within 30 days. Sensitivity of the rule was 95.1% (95% confidence interval [CI] = 88.0% to 98.7%), specificity was 56.3% (95% CI = 52.0% to 60.7%), positive prediction value was 25.9% (95% CI = 21.0% to 31.0%), and negative prediction value was 98.6% (95% CI = 96.5% to 99.6%).

Conclusions:  This study showed a lower sensitivity and higher specificity when applying the Vancouver Chest Pain Rule to this population as compared to the original study.
Probabilistic Classification of Age by Third Molar Development: The Use of Soft Evidence
Authors: Corradi Fabio , Pinchi Vilma , Barsanti Iljà , Garatti Stefano
Published: July 2012

Abstract:  This study introduces a method to classify individuals according to an age threshold, given sex and third molars’ dental maturity measured on the Demirjian scale by expressing uncertainty on dental evidence (soft evidence). We introduced a procedure to learn the parameters of the Naïve Bayes model, and we discussed two classification rules. The model was estimated and tested on 559 Italians aged 16–22. Two experts provided the dental evaluations, and the model was estimated for each of them. We evaluated the coherence of the evidence provided by the experts. Some indexes have been proposed to evaluate the effectiveness of the models, emphasizing how the experts’ ability and the technology affect the results. We introduced two benchmarks, one based on the sample distribution per sex and age: in this case, probability of correct classification increases 22% and the proportion of false adults impressively decreases 80.2%; the other benchmark, obtained by simulating hard evidence, shows how the use of soft evidence increases the proportion of correct classification 3.1% and decreases the crucial proportion of false adults about 20%. Similarly, the proportion of false minors decreases about 5.3%.
Effect of Sex Offenders Treatment Program on Cognitive and Emotional Characteristics of Mentally Ill Sex Offenders
Authors: Kim Jeong‐Hyun , Choi Sang Sub , Rhee Moon Sung , Kim Sun Bum , Joung Ji Sook , Kim Eun Hye
Published: July 2012

This study assessed the effect of a 10‐week cognitive behavior treatment program in 30 mentally ill sex offenders. The effect of the program was evaluated using the Interpersonal Responsiveness Index (IRI), UCLA Loneliness Scale (UCLALS), Coping Using Sex Inventory (CUSI), and Rape Myth Acceptance Scale (RMAS). Data were analyzed using the paired t‐test. The ability of sex offenders to cope with sexual acts when they faced stressful situations and to accept the rape myth was significantly improved on CUSI (t = 2.09, p = 0.04) and RMAS (t = 5.45, p < 0.001). Feelings of isolation and the ability to empathize based on IRI (t = 0.62, p = 0.54) and UCLALS (t = 0.88, p = 0.38) were not significantly improved. To prevent recidivism, treatment for mentally ill sex offenders should focus on changes in their cognitive and emotional characteristics in addition to their main psychiatric illness.
Immediate Restoration of Single Tapered Implants with Nonoccluding Provisional Crowns: A 5‐Year Clinical Prospective Study
Authors: Lang Lisa A. , Turkyilmaz Ilser , Edgin Wendell A. , Verrett Ronald , Garcia Lily T.
Published: July 2012

Background

Dental literature has limited number of publications regarding long‐term outcome data of immediate restoration of single missing teeth with an implant‐supported provisional crown.

Purpose

This 5‐year study evaluated hard and soft tissue responses to the immediate placement of single implant‐supported provisional crowns.

Materials and Methods

Twenty patients received one dental implant restored immediately with a provisional acrylic resin screw‐retained crown. Crestal bone loss was evaluated from standardized periapical radiographs collected at 3‐month intervals for the first 21 months followed by a 5‐year evaluation. Historical controls acquired from available dental literature were used for comparison.

Results

One implant failed within 2 months of surgical placement, presenting with pain and mobility. The remaining implants demonstrated no infection, pain, or radiolucencies. Nineteen implants were clinically immobile, osseointegrated, and asymptomatic at 21 months. At 5 years, one patient died, three patients were noncompliant, and 15 implants were evaluated as functional. Mean bone loss (MBL) at 1 year and 21 months was approximately 0.5 ± 0.5 mm and 0.70 ± 0.26 mm at 5 years. There was no statistically significant difference between MBLs at p < 0.05.

Conclusions

Immediate provisionalization of single dental implants compares favorably with conventional loading protocols. Long‐term data suggest that immediate provisionalization of single dental implants is a viable treatment option.
Implants with Original and Non‐Original Abutment Connections
Authors: Gigandet Michel , Bigolin Gianni , Faoro Francisco , Bürgin Walter , Brägger Urs
Published: July 2012

Aim

To test in vitro the mechanical resistance, rotational misfit and failure mode of three original implant‐abutment connections and to compare them to two connections between non‐original abutments connected to one of the original implants.

Material and Methods

Three different implants with small diameters (3.3 mm for Straumann Roxolid, 3.5 mm for Nobel Biocare Replace and Astra Tech Osseospeed TX) were connected with individualized titanium abutments.

Twelve implants from each system were connected to their original abutments (Straumann CARES, Nobel Biocare Procera, Astra Tech Atlantis). Twenty‐four Roxolid implants were connected with non‐original abutments using CAD/CAM procedures from the other two manufacturers (12 Nobel Biocare Procera and 12 Astra Tech Atlantis). For the critical bending test, a Zwick/Roell 1475 machine and the Xpert Zwick/Roell software were used.

Results

The rotational misfit varied when comparing the different interfaces. The use of non‐original grade V titanium abutments on Roxolid implants increased the force needed for deformation. The fracture mode was different with one of the original connections.

Conclusions

Non‐original abutments differ in design of the connecting surfaces and material and demonstrate higher rotational misfit. These differences may result in unexpected failure modes.