bluuebunny/crossref_metadata_2025 huggingface dataset:
Liquid Biopsy in Cervical Cancer: Hopes and Pitfalls
Authors: Cafforio Paola , Palmirotta Raffaele , Lovero Domenica , Cicinelli Ettore , Cormio Gennaro , Silvestris Erica , Porta Camillo , D’Oronzo Stella
Published: August 2021

Cervical cancer (CC) is the fourth most common cancer in women worldwide, with about 90% of cancer-related deaths occurring in developing countries. The geographical influence on disease evolution reflects differences in the prevalence of human papilloma virus (HPV) infection, which is the main cause of CC, as well as in the access and quality of services for CC prevention and diagnosis. At present, the most diffused screening and diagnostic tools for CC are Papanicolaou test and the more sensitive HPV-DNA test, even if both methods require gynecological practices whose acceptance relies on the woman’s cultural and religious background. An alternative (or complimentary) tool for CC screening, diagnosis, and follow-up might be represented by liquid biopsy. Here, we summarize the main methodologies developed in this context, including circulating tumor cell detection and isolation, cell tumor DNA sequencing, coding and non-coding RNA detection, and exosomal miRNA identification. Moreover, the pros and cons of each method are discussed, and their potential applications in diagnosis and prognosis of CC, as well as their role in treatment monitoring, are explored. In conclusion, it is evident that despite many advances obtained in this field, further effort is needed to validate and standardize the proposed methodologies before any clinical use.
The Bateman-Type Soft Tissue Reconstruction around Proximal or Total Humeral Megaprostheses in Patients with Primary Malignant Bone Tumors—Functional Outcome and Endoprosthetic Complications
Authors: Ahrens Helmut , Theil Christoph , Gosheger Georg , Rödl Robert , Deventer Niklas , Rickert Carolin , Ackmann Thomas , Schwarze Jan , Klingebiel Sebastian , Schneider Kristian Nikolaus
Published: August 2021

We aimed to evaluate the functional outcome and endoprosthetic complications following the Bateman-type soft tissue reconstruction around proximal or total humeral replacements in patients undergoing resection of a primary malignant bone tumor. Between September 2001 and December 2018, a total of 102 patients underwent resection of a primary malignant bone tumor and subsequent reconstruction with a modular humeral megaprosthesis in our department. Fifteen (15%) of these patients underwent a Bateman-type soft tissue reconstruction and were included in this retrospective study. The median Musculoskeletal Tumor Society (MSTS) score was 21, the median Toronto Extremity Salvage Score (TESS) was 70, and the median American Shoulder and Elbow Surgeons (ASES) score was 72. Fifty-three percent (8/15) of all patients required a revision surgery after a median time of 6 months. There were 2 soft tissue failures, 3 infections and 3 tumor recurrences. The revision-free implant survivorship amounted to 53% (95% confidence interval (CI) 28–81) after 1 year and 47% (95% CI 22–73) at last follow-up. The Bateman-type reconstruction is a feasible option for soft tissue reconstruction but functional outcome is overall limited and the risk for revision surgery within the first postoperative year is high.
Context-Specific Efficacy of Apalutamide Therapy in Preclinical Models of Pten-Deficient Prostate Cancer
Authors: De Velasco Marco A. , Kura Yurie , Ando Naomi , Sako Noriko , Banno Eri , Fujita Kazutoshi , Nozawa Masahiro , Yoshimura Kazuhiro , Sakai Kazuko , Yoshikawa Kazuhiro , Nishio Kazuto , Uemura Hirotsugu
Published: August 2021

Significant improvements with apalutamide, a nonsteroidal antiandrogen used to treat patients suffering from advanced prostate cancer (PCa), have prompted evaluation for additional indications and therapeutic development with other agents; however, persistent androgen receptor (AR) signaling remains problematic. We used autochthonous mouse models of Pten-deficient PCa to examine the context-specific antitumor activity of apalutamide and profile its molecular responses. Overall, apalutamide showed potent antitumor activity in both early-stage and late-stage models of castration-naïve prostate cancer (CNPC). Molecular profiling by Western blot and immunohistochemistry associated persistent surviving cancer cells with upregulated AKT signaling. While apalutamide was ineffective in an early-stage model of castration-resistant prostate cancer (CRPC), it tended to prolong survival in late-stage CRPC. Molecular features associated with surviving cancer cells in CRPC included upregulated aberrant-AR, and phosphorylated S6 and proline-rich Akt substrate of 40 kDa (PRAS40). Strong synergy was observed with the pan-AKT inhibitor GSK690693 and apalutamide in vitro against the CNPC- and CRPC-derived cell lines and tended to improve the antitumor responses in CNPC but not CRPC in vivo. Upregulation of signal transducer and activator of transcription 3 (STAT3) and proviral insertion in murine-1 (PIM-1) were associated with combined apalutamide/GSK690693. Our findings show that apalutamide can attenuate Pten-deficient PCa in a context-specific manner and provides data that can be used to further study and, possibly, develop additional combinations with apalutamide.
Stem Cells: Innovative Therapeutic Options for Neurodegenerative Diseases?
Authors: Bonaventura Gabriele , Munafò Antonio , Bellanca Carlo Maria , La Cognata Valentina , Iemmolo Rosario , Attaguile Giuseppe Antonino , Di Mauro Rosaria , Di Benedetto Giulia , Cantarella Giuseppina , Barcellona Maria Luisa , Cavallaro Sebastiano , Bernardini Renato
Published: August 2021

Neurodegenerative diseases are characterized by the progressive loss of structure and/or function of both neurons and glial cells, leading to different degrees of pathology and loss of cognition. The hypothesis of circuit reconstruction in the damaged brain via direct cell replacement has been pursued extensively so far. In this context, stem cells represent a useful option since they provide tissue restoration through the substitution of damaged neuronal cells with exogenous stem cells and create a neuro-protective environment through the release of bioactive molecules for healthy neurons, as well. These peculiar properties of stem cells are opening to potential therapeutic strategies for the treatment of severe neurodegenerative disorders, for which the absence of effective treatment options leads to an increasingly socio-economic burden. Currently, the introduction of new technologies in the field of stem cells and the implementation of alternative cell tissues sources are pointing to exciting frontiers in this area of research. Here, we provide an update of the current knowledge about source and administration routes of stem cells, and review light and shadows of cells replacement therapy for the treatment of the three main neurodegenerative disorders (Amyotrophic lateral sclerosis, Parkinson’s, and Alzheimer’s disease).
Balanced Chromosomal Rearrangements Associated with Hypoprolificacy in Australian Boars (Sus scrofa domesticus)
Authors: Shams Foyez , D’Souza Darryl , Ezaz Tariq
Published: August 2021

Balanced chromosomal rearrangements, mainly reciprocal translocations, are considered to be the causative agent of several clinical conditions in farmed pigs, resulting in hypoprolificacy and economic losses. Literature suggests that reciprocal translocations are heritable and can occur de novo. The prevalence rate of these balanced structural rearrangements of chromosomes differs from country to country and varies between 0.5% and 3.3%. The Australian pig population is descendent of a small founder population and has since been a closed genetic group since the 1980s. Hence, any incident of reciprocal translocation along with the pedigree of boars that contribute sperm for artificial insemination has the potential to have an economic consequence. To date, there has been no published account for screening of reciprocal translocation associated with hypoprolificacy in the Australian pig population. In this study, we performed standard and molecular cytogenetic analyses to identify evidence of chromosome rearrangements and their association with hypoprolificacy in a representative 94 boar samples from a commercial nucleus herd. We identified three novel rearrangements between chromosomes 5 and 14, between chromosomes 9 and 10, and between chromosomes 10 and 12. In addition, we also detected a reciprocal translocation between chromosomes 3 and 16 that has previously been detected in pig herds in France. The prevalence rate was 6.38% within the samples used in this study. All four rearrangements were found to have an association with hypoprolificacy. Further study and routine monitoring will be necessary to identify any further rearrangements that will allow breeders to prevent the propagation of reciprocal translocations from generation to generation within the Australian pig population.
Organellar Introns in Fungi, Algae, and Plants
Authors: Mukhopadhyay Jigeesha , Hausner Georg
Published: August 2021

Introns are ubiquitous in eukaryotic genomes and have long been considered as ‘junk RNA’ but the huge energy expenditure in their transcription, removal, and degradation indicate that they may have functional significance and can offer evolutionary advantages. In fungi, plants and algae introns make a significant contribution to the size of the organellar genomes. Organellar introns are classified as catalytic self-splicing introns that can be categorized as either Group I or Group II introns. There are some biases, with Group I introns being more frequently encountered in fungal mitochondrial genomes, whereas among plants Group II introns dominate within the mitochondrial and chloroplast genomes. Organellar introns can encode a variety of proteins, such as maturases, homing endonucleases, reverse transcriptases, and, in some cases, ribosomal proteins, along with other novel open reading frames. Although organellar introns are viewed to be ribozymes, they do interact with various intron- or nuclear genome-encoded protein factors that assist in the intron RNA to fold into competent splicing structures, or facilitate the turn-over of intron RNAs to prevent reverse splicing. Organellar introns are also known to be involved in non-canonical splicing, such as backsplicing and trans-splicing which can result in novel splicing products or, in some instances, compensate for the fragmentation of genes by recombination events. In organellar genomes, Group I and II introns may exist in nested intronic arrangements, such as introns within introns, referred to as twintrons, where splicing of the external intron may be dependent on splicing of the internal intron. These nested or complex introns, with two or three-component intron modules, are being explored as platforms for alternative splicing and their possible function as molecular switches for modulating gene expression which could be potentially applied towards heterologous gene expression. This review explores recent findings on organellar Group I and II introns, focusing on splicing and mobility mechanisms aided by associated intron/nuclear encoded proteins and their potential roles in organellar gene expression and cross talk between nuclear and organellar genomes. Potential application for these types of elements in biotechnology are also discussed.
The Genes Encoding Small Leucine-Rich Proteoglycans Undergo Differential Expression Alterations in Colorectal Cancer, Depending on Tumor Location
Authors: Solis-Hernandez Maria Pilar , Martín Carla , García Beatriz , Pérez-López Natalia , García-Mesa Yolanda , González-Fernández Sara , García-Suárez Olivia , Merayo Jesús , Fernández-Vega Iván , Quirós Luis M.
Published: August 2021

Small leucine-rich proteoglycans (SLRPs) regulate different processes and undergo significant alterations in various diseases. Colon carcinomas (CCs) are heterogeneous pathologies with important clinical and molecular differences depending on their location, which makes it interesting to analyze the alterations in SLRPs in right- and left-sided tumors (RS- and LSCCs). SLRP transcription levels were studied in 32 CCs using qPCR compared to healthy colon mucosae samples from the same patients, 20 of them from LSCCs and the remaining 12 from RSCCs. Protein expression of genes with significant differences in their transcriptions was analyzed by immunohistochemistry. The alterations observed were related to survival data. The arrangement of transcription of SLRPs was quite similar in ascending and descending colon, but RS- and LSCCs displayed different patterns of alteration, with a greater number of deregulations occurring in the latter. The analysis of protein expression also indicated changes in the location of these molecules, largely moving to the cell interior. While podocan underexpression showed a trend toward better outcomes, no differences were observed in terms of overall survival. In vitro studies using the HT29 tumor cell line suggest that deregulation of SLRPs could affect cell proliferation. SLRPs constitute new differential markers of RS- and LSCCs, showing differences dependent on the anatomical location of the tumor.
Role of High Voltage-Gated Ca2+ Channel Subunits in Pancreatic β-Cell Insulin Release. From Structure to Function
Authors: Tuluc Petronel , Theiner Tamara , Jacobo-Piqueras Noelia , Geisler Stefanie M.
Published: August 2021

The pancreatic islets of Langerhans secrete several hormones critical for glucose homeostasis. The β-cells, the major cellular component of the pancreatic islets, secrete insulin, the only hormone capable of lowering the plasma glucose concentration. The counter-regulatory hormone glucagon is secreted by the α-cells while δ-cells secrete somatostatin that via paracrine mechanisms regulates the α- and β-cell activity. These three peptide hormones are packed into secretory granules that are released through exocytosis following a local increase in intracellular Ca2+ concentration. The high voltage-gated Ca2+ channels (HVCCs) occupy a central role in pancreatic hormone release both as a source of Ca2+ required for excitation-secretion coupling as well as a scaffold for the release machinery. HVCCs are multi-protein complexes composed of the main pore-forming transmembrane α1 and the auxiliary intracellular β, extracellular α2δ, and transmembrane γ subunits. Here, we review the current understanding regarding the role of all HVCC subunits expressed in pancreatic β-cell on electrical activity, excitation-secretion coupling, and β-cell mass. The evidence we review was obtained from many seminal studies employing pharmacological approaches as well as genetically modified mouse models. The significance for diabetes in humans is discussed in the context of genetic variations in the genes encoding for the HVCC subunits.
Anisotropic Strange Star in 5D Einstein-Gauss-Bonnet Gravity
Authors: Jasim Mahmood Khalid , Maurya Sunil Kumar , Singh Ksh. Newton , Nag Riju
Published: August 2021

In this paper, we investigated a new anisotropic solution for the strange star model in the context of 5D Einstein-Gauss-Bonnet (EGB) gravity. For this purpose, we used a linear equation of state (EOS), in particular pr=βρ+γ, (where β and γ are constants) together with a well-behaved ansatz for gravitational potential, corresponding to a radial component of spacetime. In this way, we found the other gravitational potential as well as main thermodynamical variables, such as pressures (both radial and tangential) with energy density. The constant parameters of the anisotropic solution were obtained by matching a well-known Boulware-Deser solution at the boundary. The physical viability of the strange star model was also tested in order to describe the realistic models. Moreover, we studied the hydrostatic equilibrium of the stellar system by using a modified TOV equation and the dynamical stability through the critical value of the radial adiabatic index. The mass-radius relationship was also established for determining the compactness and surface redshift of the model, which increases with the Gauss-Bonnet coupling constant α but does not cross the Buchdahal limit.
Does the Increase in Renewable Energy Influence GDP Growth? An EU-28 Analysis
Authors: Sahlian Daniela Nicoleta , Popa Adriana Florina , Creţu Raluca Florentina
Published: August 2021

The aim of our study was to analyze whether the increase in the use of renewable energy can help GDP growth. The research carried out shows that renewable energy has the ability to decrease or neutralize the negative impact of greenhouse gases (GHG), but also to maintain economic growth. We focused our analysis on the EU-28 as we know that the EU Commission’s aim, in the near future, is to join forces to reduce the GHG used and move to renewable sources. We used a panel analysis with data between 2000 and 2019 from all Member States, and our results showed that their economic growth is influenced positively by the production of renewable energy, the GHG per capita, and the GHG intensity per GDP.
Wave Energy in the Mediterranean Sea: Resource Assessment, Deployed WECs and Prospects
Authors: Dialyna Evangelia , Tsoutsos Theocharis
Published: August 2021

A detailed review of wave energy resource assessment and the state-of-the-art of deployed wave energy converters (WECs) in real environmental conditions in the Mediterranean Sea have been analysed in this study. The installed power of the several deployed WECs in the Mediterranean Sea varies between 3–2500 kW. Ten project cases of deployed WECs in the basin are presented, with their analysis of the essential features. Five different types of WEC have already been tested under real environmental conditions in Italy, Greece, Israel and Gibraltar, with Italy being the Mediterranean country with the most deployed WECs. The main questions of the relevant studies were the ongoing trends, the examination of WECs in combination with other renewable sources, the utilising of WECs for desalination, and the prospects of wave energy in the Mediterranean islands and ports. This paper is the first comprehensive study that overviews the recent significant developments in the wave energy sector in the Mediterranean countries. The research concludes that the advances of the wave energy sector in the Mediterranean Sea are significant. However, in order to commercialise WECs and wave energy exploitation to become profitable, more development is necessary.
M-SRPCNN: A Fully Convolutional Neural Network Approach for Handling Super Resolution Reconstruction on Monthly Energy Consumption Environments
Authors: de-Paz-Centeno Iván , García-Ordás María Teresa , García-Olalla Oscar , Arenas Javier , Alaiz-Moretón Héctor
Published: August 2021

We propose M-SRPCNN, a fully convolutional generative deep neural network to recover missing historical hourly data from a sensor based on the historic monthly energy consumption. The network performs a reconstruction of the load profile while keeping the overall monthly consumption, which makes it suitable to effectively replace energy apportioning systems. Experiments demonstrate that M-SRPCNN can effectively reconstruct load curves from single month overall values, outperforming traditional apportioning systems.
Advanced Exergy Analysis of Waste-Based District Heating Options through Case Studies
Authors: Ozcan Huseyin Gunhan , Hepbasli Arif , Abusoglu Aysegul , Anvari-Moghaddam Amjad
Published: August 2021

The heating of the buildings, together with domestic hot water generation, is responsible for half of the total generated heating energy, which consumes half of the final energy demand. Meanwhile, district heating systems are a powerful option to meet this demand, with their significant potential and the experience accumulated over many years. The work described here deals with the conventional and advanced exergy performance assessments of the district heating system, using four different waste heat sources by the exhaust gas potentials of the selected plants (municipal solid waste cogeneration, thermal power, wastewater treatment, and cement production), with the real-time data group based on numerical investigations. The simulated results based on conventional exergy analysis revealed that the priority should be given to heat exchanger (HE)-I, with exergy efficiency values from 0.39 to 0.58, followed by HE-II and the pump with those from 0.48 to 0.78 and from 0.81 to 0.82, respectively. On the other hand, the simulated results based on advanced exergy analysis indicated that the exergy destruction was mostly avoidable for the pump (78.32–78.56%) and mostly unavoidable for the heat exchangers (66.61–97.13%). Meanwhile, the exergy destruction was determined to be mainly originated from the component itself (endogenous), for the pump (97.50–99.45%) and heat exchangers (69.80–91.97%). When the real-time implementation was considered, the functional exergy efficiency of the entire system was obtained to be linearly and inversely proportional to the pipeline length and the average ambient temperature, respectively.
Thermodynamic Analysis and Systematic Comparison of Solar-Heated Trigeneration Systems Based on ORC and Absorption Heat Pump
Authors: García-Domínguez Jesús , Marcos J. Daniel
Published: August 2021

Modular and scalable distributed generation solutions as combined cooling, heating and power (CCHP) systems are currently a promising solution for the simultaneous generation of electricity and useful heating and cooling for large buildings or industries. In the present work, a solar-heated trigeneration approach based on different organic Rankine cycle (ORC) layouts and a single-effect H2O/LiBr absorption heat pump integrated as a bottoming cycle is analysed from the thermodynamic viewpoint. The main objective of the study is to provide a comprehensive guide for selecting the most suitable CCHP configuration for a solar-heated CCHP system, following a systematic investigation approach. Six alternative CCHP configurations based on single-pressure and dual-pressure ORC layouts, such as simple, recuperated and superheated cycles, and their combinations, and seven organic fluids as working medium are proposed and compared systematically. A field of solar parabolic trough collectors (SPTCs) used as a heat source of the ORC layouts and the absorption heat pump are kept invariant. A comprehensive parametric analysis of the different proposed configurations is carried out for different design operating conditions. Several output parameters, such as energy and exergy efficiency, net electrical power and electrical to heating and cooling ratios are examined. The study reveals that the most efficient CCHP configuration is the single-pressure ORC regenerative recuperated superheated cycle with toluene as a working fluid, which is on average 25% and 8% more efficient than the variants with single-pressure simple cycle and the dual-pressure recuperated superheated cycle, respectively. At nominal design conditions, the best performing CCHP variant presents 163.7% energy efficiency and 12.3% exergy efficiency, while the electricity, cooling and heating productions are 56.2 kW, 223.0 kW and 530.1 kW, respectively.
Non-Fragile H∞ Nonlinear Observer for State of Charge Estimation of Lithium-Ion Battery Based on a Fractional-Order Model
Authors: Zhang Zhongwei , Zhou Dan , Xiong Neng , Zhu Qiao
Published: August 2021

This paper deals with the state of charge (SOC) estimation of lithium-ion battery (LIB) in electric vehicles (EVs). In order to accurately describe the dynamic behavior of the battery, a fractional 2nd-order RC model of the battery pack is established. The factional-order battery state equations are characterized by the continuous frequency distributed model. Then, in order to ensure the effective function of nonlinear function, Lipschitz condition and unilateral Lipschitz condition are proposed to solve the problem of nonlinear output equation in the process of observer design. Next, the linear matrix (LMIS) inequality based on Lyapunov’s stability theory and H∞ method is presented as a description of the design criteria for non-fragile observer. Compared with the existing literature that adopts observers, the proposed method takes the advantages of fractional-order systems in modeling accuracy, the robustness of H∞ method in restricting the unknown variables, and the non-fragile property for tolerating slow drifts on observer gain. Finally, The LiCoO2 LIB module is utilized to verify the effectiveness of the proposed observer method in different operation conditions. Experimental results show that the maximum estimation accuracy of the proposed non-fragile observer under three different dynamic conditions is less than 2%.
The Problem of Train Scheduling in the Context of the Load on the Power Supply Infrastructure. A Case Study
Authors: Haładyn Szymon
Published: August 2021

This article deals with the new challenges facing modernising railways in Poland. We look at the problem of the efficiency of the power supply system (3 kV DC) used in the context of the increasing use of electric vehicles, which have a higher demand for electricity than the old type. We present and characterise the power supply system in use, pointing out its weaknesses. We consider a case study. The load of the power supply network generated by the rolling stock used in Poland was examined using a microsimulation. A real train timetable was taken into account on a fragment of one of the most important railway line sections in one of the urban agglomerations. Then the results were compared with the results of a microsimulation in which old units were replaced by new trains. These tests were carried out in several variants. We found critical points in the scheduling of railway system use. Our results indicate that it is becoming increasingly necessary to take into account the permissible load capacity of the supply network in certain traffic situations in the process of timetable construction.
Stability Domain Analysis and Enhancement of Squirrel Cage Induction Generator Wind Turbines in Weak Grids
Authors: Devadason Jonathan , Moses Paul S. , Masoum Mohammad A. S.
Published: August 2021

There are significant concerns regarding the stability of increased wind power generation in weak power grids. This paper investigates and improves the stability of Wind Turbine Squirrel Cage Induction Generators (WT-SCIGs) with series compensation and weak interconnections to the power grid. Detailed time-domain and state-space modeling have revealed new bifurcations and oscillatory modes for a WT-SCIG connected radially to a weak grid through a series compensated line. The stability domain analyses are carried out by computing bifurcations in the system by analyzing eigenvalues of the linearized system. The analyses demonstrate for the first time how the degree of compensation at which the Hopf bifurcation occurs depends on the X/R ratio of the line, operating slip of the induction generator, and voltage regulator parameters as well as the time delays in measurements. A new damping controller is proposed, which greatly improves the dynamic stability of the WT-SCIG and eliminates destructive Hopf bifurcations in weak grids for a wide range of series compensation. This allows for a much larger percentage of series compensation than what is usually possible, while avoiding instabilities, thereby maximizing the power transfer capability.
Overcoming the Project Communications Management Breakdown amongst Foreign Workers during the COVID-19 Pandemic in Biophilia Inveigled Construction Projects in Malaysia
Authors: Subramaniam Chitdrakantan , Ismail Syuhaida , Durdyev Serdar , Wan Mohd Rani Wan Nurul Mardiah , Bakar Nur Fatin Syazwani Abu , Banaitis Audrius
Published: August 2021

The property sector is revitalised to incorporate sustainability, specifically the biophilic design, to encourage human interaction with nature. Thus, there is an urgent need to communicate the biophilic design elements amongst stakeholders, especially the foreign workers, to ensure the project meets the requirement. However, standard project communications management is no longer practical, as the construction industry worldwide has been severely impacted by the Coronavirus Disease 2019 (COVID-19) pandemic. Hence, this paper evaluates the biophilic design elements to be communicated and examines the project communications management breakdown relating to the biophilic design elements in the Malaysian construction industry during the COVID-19 pandemic. Through a systematic literature review (SLR), focus group discussion (FGD) and questionnaire survey on 147 foreign workers, this paper found that from 33 biophilic design element items, only 1 item is categorised as highly critical, whereas another 20 items are categorised as critical. In addition, 43 potential communications management breakdown items from a total of 66 items fall under the critical category. The finding suggests that communication breakdown is caused by the language barrier amongst foreign workers from different ethnicities as well as between foreign workers and the supervisors, since face to face communication is limited during the pandemic.
The Distribution and Migration of 137Cs in Oak (Quercus serrata) and Cedar (Cryptomeria japonica) Forest Organic Fractions
Authors: Mensah Akwasi Dwira , Toda Hiroto , Bellingrath-Kimura Sonoko Dorothea , Kato Hiroaki , Choi Dongsu
Published: August 2021

To analyse the 137Cs distribution and migration under various fractions of organic matter layers, this study investigated easily recognizable, originally shaped organic L-fractions, and not easily recognizable, early fermented and fragmented organic F-fractions, of both oak (Quercus serrata) and cedar (Cryptomeria japonica) sampled from Osawa watershed sites at Nihonmatsu City, Fukushima Prefecture, Japan. The organic materials were put on top of soil columns from Field Museum (FM) Tamakyuryo in Hachioji City, Tokyo. The 137Cs vertical distribution in forest soil profiles was analyzed using the relaxation mass depth, ho (kg m−2). Soil columns with both L and F- organic layer fractions of both oak and cedar, labelled as Oak-L, Oak-F, Cedar-L and Cedar-F with four replications (n = 16), were set up by the laboratory column-based method and kept under five months’ incubation period. Soil columns after incubation were sampled at depths of 0–1 cm, 1–2 cm, 2–5 cm and 5–10 cm. Results of 137Cs inventory in the organic fractions showed that 86% (oak and cedar) of the total organic layer fractions 137Cs inventory accumulated within the F-layer, indicating that the transformation of litter is a huge source for potentially mobile 137Cs, especially the oak F-layer (67% 137Cs inventory) and further continuous transfer into the forest soil mineral layers. A higher ho in L treated soils (Oak-L and Cedar-L) compared to the F treatments implied that the low 137Cs amounts penetrated faster and deeper due to their water-soluble nature. Furthermore, Cedar-F showed a higher ho of 24.3 kg m−2 than Oak-F of ho, 14.0 kg m−2, and a significant positive relationship between 137Cs retention and total carbon (TC) (p < 0.05) suggested the influence of soil organic matter on 137Cs penetration and retention. The C/N (carbon nitrogen ratio) results revealed that organic matter fractions of high C/N including 137Cs, as observed in Cedar-F, in which decomposition does not advance, penetrates soil depths while the organic matter fraction of low C/N, observed in Oak-F, showed that decomposition advanced to release 137Cs which was held by adsorption unto the RIP (radiocesium interception potential) of soil surface. In addition, infiltration by water as a transportation process was suggested to largely influence the downward migration and retention of 137Cs at lower depths of Cedar-F.
Mechanical and Structural Characterization of Pineapple Leaf Fiber
Authors: Gaba Eric Worlawoe , Asimeng Bernard O. , Kaufmann Elsie Effah , Katu Solomon Kingsley , Foster E. Johan , Tiburu Elvis K.
Published: August 2021

Evidence-based research had shown that elevated alkali treatment of pineapple leaf fiber (PALF) compromised the mechanical properties of the fiber. In this work, PALF was subjected to differential alkali concentrations: 1, 3, 6, and 9% wt/wt to study the influence on the mechanical and crystal properties of the fiber. The crystalline and mechanical properties of untreated and alkali-treated PALF samples were investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), and tensile testing analysis. The XRD results indicated that crystal properties of the fibers were modified with 6% wt/wt alkali-treated PALF recording the highest crystallinity and crystallite size of 76% and 24 nm, respectively. The FTIR spectra suggested that all alkali-treated PALF samples underwent lignin and hemicellulose removal to varying degrees. An increase in the crystalline properties improved the mechanical properties of the PALF treated with alkali at 6% wt/wt, which has the highest tensile strength (1620 MPa). Although the elevated alkali treatment resulted in decreased mechanical properties of PALF, crystallinity generally increased. The findings revealed that the mechanical properties of PALF not only improve with increasing crystallinity and crystallite size, but are also dependent on the intermediate bond between adjacent cellulose chains.
Experimental and Numerical Analysis of Formation and Flame Precession of Fire Whirls: A Review
Authors: Ghodrat Maryam , Shakeriaski Farshad , Nelson David James , Simeoni Albert
Published: August 2021

Fire whirls are a particular case of flame behaviour characterized by a rotating column of fire driven by intense convective heating of air close to the ground. They typically result in a substantial increase in burning rate, temperature, and flame height. Fire whirls can occur in any intense flame environment, including urban areas, particularly within combustible structures, and in wildland or forest fires. Recently, investigations on the creation of fire whirls have attracted much attention. However, most analyses are focused on fire whirl structure, formation, and controlling their unique state. In effect, revisiting the available experimental techniques and numerical simulations used in analyzing fire whirls has received less attention. In this paper, experimental arrangements including empirical set ups and employed fuels are presented in detail. Subsequently, major research progress focused on experimental studies and their laboratory setup is fully discussed, followed by the available numerical simulations, including combustion and turbulence models. Applied methodologies and chosen software in the recent numerical studies are also reviewed exclusively. Finally, the latest findings are featured, and prospective pathways are advised.
Energy Saving and Charging Discharging Characteristics of Multiple PCMs Subjected to Internal Air Flow
Authors: Hamad Ahmed J.
Published: August 2021

One essential utilization of phase change materials as energy storage materials is energy saving and temperature control in air conditioning and indirect solar air drying systems. This study presents an experimental investigation evaluating the characteristics and energy savings of multiple phase change materials subjected to internal flow in an air heating system during charging and discharging cycles. The experimental tests were conducted using a test rig consisting of two main parts, an air supply duct and a room model equipped with phase change materials (PCMs) placed in rectangular aluminum panels. Analysis of the results was based on three test cases: PCM1 (Paraffin wax) placed in the air duct was used alone in the first case; PCM2 (RT–42) placed in the room model was used alone in the second case; and in the third case, the two PCMs (PCM1 and PCM2) were used at the same time. The results revealed a significant improvement in the energy savings and room model temperature control for the air heating system incorporated with multiple PCMs compared with that of a single PCM. Complete melting during the charging cycle occurred at temperatures in the range of 57–60 °C for PCM1 and 38–43 °C for PCM2, respectively, thereby validating the reported PCMs’ melting–solidification results. Multiple PCMs maintained the room air temperature at the desired range of 35–45.2 °C in the air heating applications by minimizing the air temperature fluctuations. The augmentation in discharging time and improvement in the room model temperature using multiple PCMs were about 28.4% higher than those without the use of PCMs. The total energy saving using two PCMs was higher by about 29.5% and 46.7% compared with the use of PCM1 and PCM2, respectively. It can be concluded that multiple PCMs have revealed higher energy savings and thermal stability for the air heating system considered in the current study.
Delivery of Phenolic Compounds, Peptides and β-Glucan to the Gastrointestinal Tract by Incorporating Dietary Fibre-Rich Mushrooms into Sorghum Biscuits
Authors: Tu Juncai , Brennan Margaret Anne , Wu Gang , Bai Weidong , Cheng Ping , Tian Bin , Brennan Charles Stephen
Published: August 2021

Sorghum biscuits were enriched with mushroom powders (Lentinula edodes, Auricularia auricula and Tremella fuciformis) at 5%, 10% and 15% substitution levels. An in vitro gastrointestinal digestion was used to evaluate the effect of this enrichment on the phenolic content and soluble peptide content as well as antioxidant activities of the gastric or intestinal supernatants (bio-accessible fractions), and the remaining portions of phenolic compounds, antioxidants and β-glucan in the undigested residue (non-digestible fraction). The phenolic content of the gastric and intestinal supernatants obtained from digested mushroom-enriched biscuits was found to be higher than that of control biscuit, and the phenolic content was positively correlated to the antioxidant activities in each fraction (p < 0.001). L. edodes and T. fuciformis enrichment increased the soluble protein content (small peptide) of sorghum biscuits after in vitro digestion. All mushroom enrichment increased the total phenolic content and β-glucan content of the undigested residue and they were positively correlated (p < 0.001). The insoluble dietary fibre of biscuits was positively correlated with β-glucan content (p < 0.001) of undigested residue. These findings suggested that enriching food with mushroom derived dietary fibre increases the bioavailability of the non-digestible β-glucan and phenolic compounds.
Insight into the Effect of Ice Addition on the Gel Properties of Nemipterus virgatus Surimi Gel Combined with Water Migration
Authors: Chen Haiqiang , Zou Yiqian , Zhou Aimei , Xiao Jie , Benjakul Soottawat
Published: August 2021

The effect of the amount of ice added (20–60%) on the gel properties and water migration of Nemipterus virgatus surimi gel obtained with two-stage heat treatment was studied. The gel strength and water-holding capability (WHC) of the surimi gel with 30% ice added were significantly higher than those of other treatment groups (p < 0.05). The addition of 30% ice was conducive to the increase of protein β-sheet proportion during heat treatment, exposing more reactive sulfhydryl groups. These promoted the combination of protein-protein through disulfide bonds and hydrophobic-hydrophobic interactions, forming an ordered three-dimensional gel network structure. Meanwhile, the increase in hydrogen bonds promoted the protein-water interaction. Low-field nuclear magnetic resonance analysis showed that more bound water was locked in the gel system, reducing the migration of immobile water to free water and finally showing better gel properties. When the amount of ice added was insufficient (20%), the gel structure lacked the support of immobile water, resulting in deterioration of gel strength. However, excessive addition of ice (>30%) was not conducive to the combination of protein-protein and protein-water, forming a large and rough gel structure, resulting in the migration of immobile water to free water and ultimately exhibited weak gel properties.
Physicochemical, Mineral and Sensory Characteristics of Instant Citrullus lanatus mucosospermus (Egusi) Soup
Authors: Olubi Olakunbi , Felix-Minnaar Joseline Veronica , Jideani Victoria Adaora
Published: August 2021

Defatted egusi flour offers a food option high in protein and essential micronutrients. An instant processing method was adopted in a ready-to-eat instant soup using egusi grit, hydrocolloid, and defatted flour. A D-optimal quadratic mixture model was used to study the effect of the independent variables (grit, flour, and hydrocolloid) qualities. The quadratic model was adequate to navigate the design space for taste and appearance. The numerical optimization for appearance and taste of instant soup (IES) was used to obtain the optimal soup mix of 10 g of hydrocolloid, 57.2 of defatted flour and 17 g of grits. Sixteen trace and five major mineral elements were found in the egusi soup, with a high concentration of phosphorus (1220.4, 1326.2 and 1277.9 mg/100 g), potassium (1220.4, 1326.2 and 1277.9 mg/100 g), magnesium (822.2, 905.3 and 863.70 mg/100 g), calcium (172.3, 190.9 and 183.4 mg/100 g) and iron (53.7, 57.5 and 29.5 mg/100 g), and for instant egusi soups from boiled egusi grit (IESBG), instant egusi grit from spherified grit (IESSG) and instant egusi grit from extruded grit (IESEG), respectively. The amino acid profile of instant egusi soup offers all essential amino acids necessary to nourish the body. Phosphorus content was significantly (p ≤ 0.05) high across the three soups: 1742, 1836 and 1838 mg/100 g for IESBG, IESSG, and IESEG, respectively; IESSG and IESEG were significantly (p ≤ 0.05) higher in minerals when compared with IESBG. Instant egusi soup differed significantly (p ≤ 0.05) in lightness (L*), while the redness (a*) and yellowness (b*) did not vary significantly.
Optimization of Copper Stain Removal from Marble through the Formation of Cu(II) Complexes in Agar Gels
Authors: Sansonetti Antonio , Bertasa Moira , Corti Cristina , Rampazzi Laura , Monticelli Damiano , Scalarone Dominique , Sassella Adele , Canevali Carmen
Published: August 2021

Copper complexes with different ligands (ethylenediaminetetraacetic acid, EDTA, ammonium citrate tribasic, TAC, and alanine, ALA) were studied in aqueous solutions and hydrogels with the aim of setting the optimal conditions for copper stain removal from marble by agar gels, with damage minimization. The stoichiometry and stability of copper complexes were monitored by ultraviolet-visible (UV-Vis) spectroscopy and the symmetry of Cu(II) centers in the different gel formulations was studied by electron paramagnetic resonance (EPR) spectroscopy. Cleaning effectiveness in optimized conditions was verified on marble laboratory specimens through color variations and by determining copper on gels by inductively coupled plasma-mass spectrometry (ICP-MS). Two copper complexes with TAC were identified, one having the known stoichiometry 1:1, and the other 1:2, Cu(TAC)2, never observed before. The stability of all the complexes at different pH was observed to increase with pH. At pH 10.0, the gel’s effectiveness in removing copper salts from marble was the highest in the presence of ALA, followed by EDTA, TAC, and pure agar gel. Limited damage to the marble surface was observed when gels with added EDTA and TAC were employed, whereas agar gel with ALA was determined to be the most efficient and safe cleaning material.
Identification of Copy Number Variants in a Southern Chinese Cohort of Patients with Congenital Scoliosis
Authors: Lai Wenjing , Feng Xin , Yue Ming , Cheung Prudence W. H. , Choi Vanessa N. T. , Song You-Qiang , Luk Keith D. K. , Cheung Jason Pui Yin , Gao Bo
Published: August 2021

Congenital scoliosis (CS) is a lateral curvature of the spine resulting from congenital vertebral malformations (CVMs) and affects 0.5–1/1000 live births. The copy number variant (CNV) at chromosome 16p11.2 has been implicated in CVMs and recent studies identified a compound heterozygosity of 16p11.2 microdeletion and TBX6 variant/haplotype causing CS in multiple cohorts, which explains about 5–10% of the affected cases. Here, we studied the genetic etiology of CS by analyzing CNVs in a cohort of 67 patients with congenital hemivertebrae and 125 family controls. We employed both candidate gene and family-based approaches to filter CNVs called from whole exome sequencing data. This identified 12 CNVs in four scoliosis-associated genes (TBX6, NOTCH2, DSCAM, and SNTG1) as well as eight recessive and 64 novel rare CNVs in 15 additional genes. Some candidates, such as DHX40, NBPF20, RASA2, and MYSM1, have been found to be associated with syndromes with scoliosis or implicated in bone/spine development. In particular, the MYSM1 mutant mouse showed spinal deformities. Our findings suggest that, in addition to the 16p11.2 microdeletion, other CNVs are potentially important in predisposing to CS.
DNA Methylation Dynamics in the Female Germline and Maternal-Effect Mutations That Disrupt Genomic Imprinting
Authors: Anvar Zahra , Chakchouk Imen , Demond Hannah , Sharif Momal , Kelsey Gavin , Van den Veyver Ignatia B.
Published: August 2021

Genomic imprinting is an epigenetic marking process that results in the monoallelic expression of a subset of genes. Many of these ‘imprinted’ genes in mice and humans are involved in embryonic and extraembryonic growth and development, and some have life-long impacts on metabolism. During mammalian development, the genome undergoes waves of (re)programming of DNA methylation and other epigenetic marks. Disturbances in these events can cause imprinting disorders and compromise development. Multi-locus imprinting disturbance (MLID) is a condition by which imprinting defects touch more than one locus. Although most cases with MLID present with clinical features characteristic of one imprinting disorder. Imprinting defects also occur in ‘molar’ pregnancies-which are characterized by highly compromised embryonic development-and in other forms of reproductive compromise presenting clinically as infertility or early pregnancy loss. Pathogenic variants in some of the genes encoding proteins of the subcortical maternal complex (SCMC), a multi-protein complex in the mammalian oocyte, are responsible for a rare subgroup of moles, biparental complete hydatidiform mole (BiCHM), and other adverse reproductive outcomes which have been associated with altered imprinting status of the oocyte, embryo and/or placenta. The finding that defects in a cytoplasmic protein complex could have severe impacts on genomic methylation at critical times in gamete or early embryo development has wider implications beyond these relatively rare disorders. It signifies a potential for adverse maternal physiology, nutrition, or assisted reproduction to cause epigenetic defects at imprinted or other genes. Here, we review key milestones in DNA methylation patterning in the female germline and the embryo focusing on humans. We provide an overview of recent findings regarding DNA methylation deficits causing BiCHM, MLID, and early embryonic arrest. We also summarize identified SCMC mutations with regard to early embryonic arrest, BiCHM, and MLID.