bluuebunny/crossref_metadata_2025 huggingface dataset:
Statistical Optimisation of Phenol Degradation and Pathway Identification through Whole Genome Sequencing of the Cold-Adapted Antarctic Bacterium, Rhodococcus sp. Strain AQ5-07
Authors: Lee Gillian Li Yin , Zakaria Nur Nadhirah , Convey Peter , Futamata Hiroyuki , Zulkharnain Azham , Suzuki Kenshi , Abdul Khalil Khalilah , Shaharuddin Noor Azmi , Alias Siti Aisyah , González-Rocha Gerardo , Ahmad Siti Aqlima
Published: December 2020

Study of the potential of Antarctic microorganisms for use in bioremediation is of increasing interest due to their adaptations to harsh environmental conditions and their metabolic potential in removing a wide variety of organic pollutants at low temperature. In this study, the psychrotolerant bacterium Rhodococcus sp. strain AQ5-07, originally isolated from soil from King George Island (South Shetland Islands, maritime Antarctic), was found to be capable of utilizing phenol as sole carbon and energy source. The bacterium achieved 92.91% degradation of 0.5 g/L phenol under conditions predicted by response surface methodology (RSM) within 84 h at 14.8 °C, pH 7.05, and 0.41 g/L ammonium sulphate. The assembled draft genome sequence (6.75 Mbp) of strain AQ5-07 was obtained through whole genome sequencing (WGS) using the Illumina Hiseq platform. The genome analysis identified a complete gene cluster containing catA, catB, catC, catR, pheR, pheA2, and pheA1. The genome harbours the complete enzyme systems required for phenol and catechol degradation while suggesting phenol degradation occurs via the β-ketoadipate pathway. Enzymatic assay using cell-free crude extract revealed catechol 1,2-dioxygenase activity while no catechol 2,3-dioxygenase activity was detected, supporting this suggestion. The genomic sequence data provide information on gene candidates responsible for phenol and catechol degradation by indigenous Antarctic bacteria and contribute to knowledge of microbial aromatic metabolism and genetic biodiversity in Antarctica.
UBAC1/KPC2 Regulates TLR3 Signaling in Human Keratinocytes through Functional Interaction with the CARD14/CARMA2sh-TANK Complex
Authors: Mazzone Pellegrino , Congestrì Michele , Scudiero Ivan , Polvere Immacolata , Voccola Serena , Zerillo Lucrezia , Telesio Gianluca , Vito Pasquale , Stilo Romania , Zotti Tiziana
Published: December 2020

CARD14/CARMA2 is a scaffold molecule whose genetic alterations are linked to human inherited inflammatory skin disorders. However, the mechanisms through which CARD14/CARMA2 controls innate immune response and chronic inflammation are not well understood. By means of a yeast two-hybrid screening, we identified the UBA Domain Containing 1 (UBAC1), the non-catalytic subunit of the E3 ubiquitin-protein ligase KPC complex, as an interactor of CARMA2sh, the CARD14/CARMA2 isoform mainly expressed in human keratinocytes. UBAC1 participates in the CARMA2sh/TANK complex and promotes K63-linked ubiquitination of TANK. In human keratinocytes, UBAC1 negatively regulates the NF-κF-activating capacity of CARMA2sh following exposure to poly (I:C), an agonist of Toll-like Receptor 3. Overall, our data indicate that UBAC1 participates in the inflammatory signal transduction pathways involving CARMA2sh.
Deciphering Adipose Tissue Extracellular Vesicles Protein Cargo and Its Role in Obesity
Authors: Camino Tamara , Lago-Baameiro Nerea , Martis-Sueiro Aurelio , Couto Iván , Santos Francisco , Baltar Javier , Pardo María
Published: December 2020

The extracellular vesicles (EVs) have emerged as key players in metabolic disorders rising as an alternative way of paracrine/endocrine communication. In particular, in relation to adipose tissue (AT) secreted EVs, the current knowledge about its composition and function is still very limited. Nevertheless, those vesicles have been lately suggested as key players in AT communication at local level, and also with other metabolic peripheral and central organs participating in physiological homoeostasis, and also contributing to the metabolic deregulation related to obesity, diabetes, and associated comorbidities. The aim of this review is to summarize the most relevant data around the EVs secreted by adipose tissue, and especially in the context of obesity, focusing in its protein cargo. The description of the most frequent proteins identified in EVs shed by AT and its components, including their changes under pathological status, will give the reader a whole picture about the membrane/antigens, and intracellular proteins known so far, in an attempt to elucidate functional roles, and also suggesting biomarkers and new paths of therapeutic action.
Dichloroacetate Radiosensitizes Hypoxic Breast Cancer Cells
Authors: de Mey Sven , Dufait Inès , Jiang Heng , Corbet Cyril , Wang Hui , Van De Gucht Melissa , Kerkhove Lisa , Law Ka Lun , Vandenplas Hugo , Gevaert Thierry , Feron Olivier , De Ridder Mark
Published: December 2020

Mitochondrial metabolism is an attractive target for cancer therapy. Reprogramming metabolic pathways can potentially sensitize tumors with limited treatment options, such as triple-negative breast cancer (TNBC), to chemo- and/or radiotherapy. Dichloroacetate (DCA) is a specific inhibitor of the pyruvate dehydrogenase kinase (PDK), which leads to enhanced reactive oxygen species (ROS) production. ROS are the primary effector molecules of radiation and an increase hereof will enhance the radioresponse. In this study, we evaluated the effects of DCA and radiotherapy on two TNBC cell lines, namely EMT6 and 4T1, under aerobic and hypoxic conditions. As expected, DCA treatment decreased phosphorylated pyruvate dehydrogenase (PDH) and lowered both extracellular acidification rate (ECAR) and lactate production. Remarkably, DCA treatment led to a significant increase in ROS production (up to 15-fold) in hypoxic cancer cells but not in aerobic cells. Consistently, DCA radiosensitized hypoxic tumor cells and 3D spheroids while leaving the intrinsic radiosensitivity of the tumor cells unchanged. Our results suggest that although described as an oxidative phosphorylation (OXPHOS)-promoting drug, DCA can also increase hypoxic radioresponses. This study therefore paves the way for the targeting of mitochondrial metabolism of hypoxic cancer cells, in particular to combat radioresistance.
Unraveling the Role of Leptin in Liver Function and Its Relationship with Liver Diseases
Authors: Martínez-Uña Maite , López-Mancheño Yaiza , Diéguez Carlos , Fernández-Rojo Manuel A. , Novelle Marta G.
Published: December 2020

Since its discovery twenty-five years ago, the fat-derived hormone leptin has provided a revolutionary framework for studying the physiological role of adipose tissue as an endocrine organ. Leptin exerts pleiotropic effects on many metabolic pathways and is tightly connected with the liver, the major player in systemic metabolism. As a consequence, understanding the metabolic and hormonal interplay between the liver and adipose tissue could provide us with new therapeutic targets for some chronic liver diseases, an increasing problem worldwide. In this review, we assess relevant literature regarding the main metabolic effects of leptin on the liver, by direct regulation or through the central nervous system (CNS). We draw special attention to the contribution of leptin to the non-alcoholic fatty liver disease (NAFLD) pathogenesis and its progression to more advanced stages of the disease as non-alcoholic steatohepatitis (NASH). Likewise, we describe the contribution of leptin to the liver regeneration process after partial hepatectomy, the mainstay of treatment for certain hepatic malignant tumors.
Molecular Biology of Atherosclerotic Ischemic Strokes
Authors: Tuttolomondo Antonino , Puleo Maria Grazia , Velardo Maria Chiara , Corpora Francesca , Daidone Mario , Pinto Antonio
Published: December 2020

Among the causes of global death and disability, ischemic stroke (also known as cerebral ischemia) plays a pivotal role, by determining the highest number of worldwide mortality, behind cardiomyopathies, affecting 30 million people. The etiopathogenetic burden of a cerebrovascular accident could be brain ischemia (~80%) or intracranial hemorrhage (~20%). The most common site when ischemia occurs is the one is perfused by middle cerebral arteries. Worse prognosis and disablement consequent to brain damage occur in elderly patients or affected by neurological impairment, hypertension, dyslipidemia, and diabetes. Since, in the coming years, estimates predict an exponential increase of people who have diabetes, the disease mentioned above constitutes together with stroke a severe social and economic burden. In diabetic patients after an ischemic stroke, an exorbitant activation of inflammatory molecular pathways and ongoing inflammation is responsible for more severe brain injury and impairment, promoting the advancement of ischemic stroke and diabetes. Considering that the ominous prognosis of ischemic brain damage could by partially clarified by way of already known risk factors the auspice would be modifying poor outcome in the post-stroke phase detecting novel biomolecules associated with poor prognosis and targeting them for revolutionary therapeutic strategies.
Inhibition of the FGF/FGFR System Induces Apoptosis in Lung Cancer Cells via c-Myc Downregulation and Oxidative Stress
Authors: Giacomini Arianna , Taranto Sara , Rezzola Sara , Matarazzo Sara , Grillo Elisabetta , Bugatti Mattia , Scotuzzi Alessia , Guerra Jessica , Di Trani Martina , Presta Marco , Ronca Roberto
Published: December 2020

Lung cancer represents an extremely diffused neoplastic disorder with different histological/molecular features. Among the different lung tumors, non-small-cell lung cancer (NSCLC) is the most represented histotype, characterized by various molecular markers, including the expression/overexpression of the fibroblast growth factor receptor-1 (FGFR1). Thus, FGF/FGFR blockade by tyrosine kinase inhibitors (TKi) or FGF-ligand inhibitors may represent a promising therapeutic approach in lung cancers. In this study we demonstrate the potential therapeutic benefit of targeting the FGF/FGFR system in FGF-dependent lung tumor cells using FGF trapping (NSC12) or TKi (erdafitinib) approaches. The results show that inhibition of FGF/FGFR by NSC12 or erdafitinib induces apoptosis in FGF-dependent human squamous cell carcinoma NCI-H1581 and NCI-H520 cells. Induction of oxidative stress is the main mechanism responsible for the therapeutic/pro-apoptotic effect exerted by both NSC12 and erdafitinib, with apoptosis being abolished by antioxidant treatments. Finally, reduction of c-Myc protein levels appears to strictly determine the onset of oxidative stress and the therapeutic response to FGF/FGFR inhibition, indicating c-Myc as a key downstream effector of FGF/FGFR signaling in FGF-dependent lung cancers.
Structural Basis for Design of New Purine-Based Inhibitors Targeting the Hydrophobic Binding Pocket of Hsp90
Authors: Shin Sang Chul , El-Damasy Ashraf K. , Lee Ju Hyeon , Seo Seon Hee , Kim Ji Hyun , Seo Young Ho , Lee Yuri , Yu Ji Hoon , Bang Eun Kyoung , Kim Eunice EunKyeong , Keum Gyochang
Published: December 2020

Inhibition of the molecular chaperone heat shock protein 90 (Hsp90) represents a promising approach for cancer treatment. BIIB021 is a highly potent Hsp90 inhibitor with remarkable anticancer activity; however, its clinical application is limited by lack of potency and response. In this study, we aimed to investigate the impact of replacing the hydrophobic moiety of BIIB021, 4-methoxy-3,5-dimethylpyridine, with various five-membered ring structures on the binding to Hsp90. A focused array of N7/N9-substituted purines, featuring aromatic and non-aromatic rings, was designed, considering the size of hydrophobic pocket B in Hsp90 to obtain insights into their binding modes within the ATP binding site of Hsp90 in terms of π–π stacking interactions in pocket B as well as outer α-helix 4 configurations. The target molecules were synthesized and evaluated for their Hsp90α inhibitory activity in cell-free assays. Among the tested compounds, the isoxazole derivatives 6b and 6c, and the sole six-membered derivative 14 showed favorable Hsp90α inhibitory activity, with IC50 values of 1.76 µM, 0.203 µM, and 1.00 µM, respectively. Furthermore, compound 14 elicited promising anticancer activity against MCF-7, SK-BR-3, and HCT116 cell lines. The X-ray structures of compounds 4b, 6b, 6c, 8, and 14 bound to the N-terminal domain of Hsp90 were determined in order to understand the obtained results and to acquire additional structural insights, which might enable further optimization of BIIB021.
Correction: Yagüe, P., et al. Goals and Challenges in Bacterial Phosphoproteomics. Int. J. Mol. Sci. 2019, 20, 5678
Authors: Yagüe Paula , Gonzalez-Quiñonez Nathaly , Fernández-García Gemma , Alonso-Fernández Sergio , Manteca Angel
Published: December 2020

The authors wish to make the following corrections to this paper [1]:The author name “Gemma Fernánez-García” should be “Gemma Fernández-García” [...]
Optimization of Power and Levelized Cost for Shrouded Small Wind Turbine
Authors: Khojasteh Hasanali , Noorollahi Younes , Tahani Mojtaba , Masdari Mehran
Published: December 2020

Nowadays, by increasing energy demand and considering the importance of environmental issues in recent decades, the use of renewable energies is expanding. Among renewable energies, wind power and its technology are growing and evolving more rapidly. Resource assessment in Iran has revealed the significant potential of wind energy around the country. To further develop wind energy in the country and create large-scale wind power plants, the consideration of distributed power generation using small wind turbines for applications in agricultural and residential use is needed. Conventional small wind turbines and small wind lens turbines have been developed in recent years. In this research project, a small wind lens turbine is designed. The advantages of this turbine are an increased production capacity and reduced cut-in speed and noise pollution. In this study, a lens (or shroud) is added to a small turbine, and the maximized annual energy production (AEP) and minimization of the levelized cost of energy (LCOE) are modeled. We applied the NSGA-II algorithm for optimization to find the best answer. The input parameters in the objective function of the AEP are cut-in, cut-out, rated speeds, scale factor, and shape factor. Additionally, the input parameters in the objective function of the LCOE are the power production, initial capital cost, annual operating expenses, and balance of energy. The results indicate that installing a wind lens turbine in Kish Island led to an LCOE decrease of 56% on average, and we can see an 83% increase in the AEP. In the Firoozkooh area, an average reduction of 59% in the LCOE and 74% increase in the AEP for a wind lens turbine is observed.
Hybrid Additive Manufacturing of Collector Coins
Authors: Pragana João P. M. , Rosenthal Stephan , Bragança Ivo M. F. , Silva Carlos M. A. , Tekkaya A. Erman , Martins Paulo A. F.
Published: December 2020

The objective of this paper is to present a new hybrid additive manufacturing route for fabricating collector coins with complex, intricate contoured holes. The new manufacturing route combines metal deposition by additive manufacturing with metal cutting and forming, and its application is illustrated with an example consisting of a prototype coin made from stainless steel AISI 316L. Experimentation and finite element analysis of the coin minting operation with the in-house computer program i-form show that the blanks produced by additive manufacturing and metal cutting can withstand the high compressive pressures that are attained during the embossing and impressing of lettering and other reliefs on the coin surfaces. The presentation allows concluding that hybrid additive manufacturing opens the way to the production of innovative collector coins with geometric features that are radically different from those that are currently available in the market.
Use of Superparamagnetic Iron Oxide Nanoparticles (SPIONs) via Multiple Imaging Modalities and Modifications to Reduce Cytotoxicity: An Educational Review
Authors: Nelson Nicholas , Port John , Pandey Mukesh
Published: December 2020

The aim of the present educational review on superparamagnetic iron oxide nanoparticles (SPIONs) is to inform and guide young scientists and students about the potential use and challenges associated with SPIONs. The present review discusses the basic concepts of magnetic resonance imaging (MRI), basic construct of SPIONs, cytotoxic challenges associated with SPIONs, shape and sizes of SPIONs, site-specific accumulation of SPIONs, various methodologies applied to reduce cytotoxicity including coatings with various materials, and application of SPIONs in targeted delivery of chemotherapeutics (Doxorubicin), biotherapeutics (DNA, siRNA), and positron emission tomography (PET) imaging applications.
Biosensors and Diagnostics for Fungal Detection
Authors: K. Hussain Khalil , Malavia Dhara , M. Johnson Elizabeth , Littlechild Jennifer , Winlove C. Peter , Vollmer Frank , Gow Neil A. R.
Published: December 2020

Early detection is critical to the successful treatment of life-threatening infections caused by fungal pathogens, as late diagnosis of systemic infection almost always equates with a poor prognosis. The field of fungal diagnostics has some tests that are relatively simple, rapid to perform and are potentially suitable at the point of care. However, there are also more complex high-technology methodologies that offer new opportunities regarding the scale and precision of fungal diagnosis, but may be more limited in their portability and affordability. Future developments in this field are increasingly incorporating new technologies provided by the use of new format biosensors. This overview provides a critical review of current fungal diagnostics and the development of new biophysical technologies that are being applied for selective new sensitive fungal biosensors to augment traditional diagnostic methodologies.
Relationship between Species Richness, Biomass and Structure of Vegetation and Mycobiota along an Altitudinal Transect in the Polar Urals
Authors: Shiryaev Anton G. , Peintner Ursula , Elsakov Vladimir V. , Sokovnina Svetlana Yu. , Kosolapov Denis A. , Shiryaeva Olga S. , Devi Nadezhda M. , Grigoriev Andrei A.
Published: December 2020

Aboveground species richness patterns of vascular plants, aphyllophoroid macrofungi, bryophytes and lichens were compared along an altitudinal gradient (80–310 m a.s.l.) on the Slantsevaya mountain at the eastern macroslope of the Polar Urals (Russia). Five altitudinal levels were included in the study: (1) Northern boreal forest with larch-spruce in the Sob’ river valley habitats; (2–3) two levels of closed, northern boreal, larch-dominated forests on the slopes; (4) crook-stemmed forest; (5) tundra habitats above the timberline. Vascular plant or bryophyte species richness was not affected by altitudinal levels, but lichen species richness significantly increased from the river valley to the tundra. For aphyllophoroid macrofungi, species richness was highest at intermediate and low altitudes, and poorest in the tundra. These results indicate a positive ecotone effect on aphyllophoroid fungal species richness. The species richness of aphyllophoroid fungi as a whole was neither correlated to mortmass stocks, nor to species richness of vascular plants, but individual ecological or morphological groups depended on these parameters. Poroid fungal species richness was positively correlated to tree age, wood biomass and crown density, and therefore peaked in the middle of the slope and at the foot of the mountain. In contrast, clavarioid fungal species richness was negatively related to woody bio- and mortmass, and therefore peaked in the tundra. This altitudinal level was characterized by high biomass proportions of lichens and mosses, and by high litter mortmass. The proportion of corticoid fungi increased with altitude, reaching its maximum at the timberline. Results from the different methods used in this work were concordant, and showed significant patterns. Tundra communities differ significantly from the forest communities, as is also confirmed by nonmetric multidimensional scaling (NMDS) analyses based on the spectrum of morphological and ecological groups of aphyllophoroid fungi.
Genomic Association of Single Nucleotide Polymorphisms with Blood Pressure Response to Hydrochlorothiazide among South African Adults with Hypertension
Authors: Masilela Charity , Pearce Brendon , Ongole Joven Jebio , Adeniyi Oladele Vincent , Benjeddou Mongi
Published: December 2020

This study described single nucleotide polymorphisms (SNPs) in hydrochlorothiazide-associated genes and further assessed their correlation with blood pressure control among South African adults living with hypertension. A total of 291 participants belonging to the Nguni tribes of South Africa on treatment for hypertension were recruited. Nineteen SNPs in hydrochlorothiazide pharmacogenes were selected and genotyped using MassArray. Uncontrolled hypertension was defined as blood pressure ≥140/90 mmHg. The association between genotypes, alleles and blood pressure response to treatment was determined by conducting multivariate logistic regression model analysis. The majority of the study participants were female (73.19%), Xhosa (54.98%) and had blood pressure ≥140/90 mmHg (68.73%). Seventeen SNPs were observed among the Xhosa tribe, and two (rs2070744 and rs7297610) were detected among Swati and Zulu participants. Furthermore, alleles T of rs2107614 (AOR = 6.69; 95%CI 1.42–31.55; p = 0.016) and C of rs2776546 (AOR = 3.78; 95%CI 1.04–13.74; p = 0.043) were independently associated with uncontrolled hypertension, whilst rs2070744 TC (AOR = 38.76; 95%CI 5.54–270.76; p = 0.00023), CC (AOR = 10.44; 95%CI 2.16–50.29; p = 0.003) and allele T of rs7297610 (AOR = 1.86; 95%CI 1.09–3.14; p = 0.023) were significantly associated with uncontrolled hypertension among Zulu and Swati participants. We confirmed the presence of SNPs associated with hydrochlorothiazide, some of which were significantly associated with uncontrolled hypertension in the study sample. Findings open doors for further studies on personalized therapy for hypertension in the country.
Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results?
Authors: DiGuardo Maggie A. , Kester Sarah J. , Mahaffey Victor J. , Hammel Scott A. , Heaser Katelyn K. , Hofich Christopher D. , Tauscher Craig D. , Kerr Sarah E. , Oliveira Jennifer L. , Jacob Eapen K. , Moyer Ann M.
Published: December 2020

Purpose: molecular testing is often indicated for recently transfused patients. However, there are no guidelines regarding the potential interference from donor DNA or whether it is necessary to wait for a period of time post-transfusion prior to genetic testing. While the majority of patients are transfused in the non-trauma setting using leukoreduced (LR) red blood cell products, the degree of leukoreduction varies among centers and is not universally practiced. Methods: whole blood units collected from anonymous donors were used in an in vitro transfusion model. One unit was split: half being leukoreduced simulating a leukopenic recipient and half left untreated. Donors were simulated by leukoreduced, partially leukoreduced (PLR), or non-leukoreduced units, transfused in 2, 5, or 16 unit equivalents. DNA from the combinations were subjected to short tandem repeat (STR) analysis for chimerism detection. Results: donor DNA was not detectable in any of the LR combinations, but detected in the PLR combinations, ranging from 0.1 to 1.5% donor DNA in the immunocompetent recipient and 6.3–27.8% in the leukopenic recipient. Non-LR donor DNA was also detected (13–95%). Conclusion: donor-derived DNA from leukoreduced blood products is unlikely to interfere with the interpretation of germline genetic testing in immunocompetent recipients but may interfere in immunocompromised recipients.
A Post-Treatment Method to Enhance the Property of Aerosol Jet Printed Electric Circuit on 3D Printed Substrate
Authors: Wang Bing , Zhang Haining , Choi Joon Phil , Moon Seung Ki , Lee Byunghoon , Koo Jamyeong
Published: December 2020

Aerosol jet printing of electronic devices is increasingly attracting interest in recent years. However, low capability and high resistance are still limitations of the printed electronic devices. In this paper, we introduce a novel post-treatment method to achieve a high-performance electric circuit. The electric circuit was printed with aerosol jet printing method on an ULTEM substrate. The ULTEM substrate was fabricated by the Fused Deposition Modelling method. After post-treatment, the electrical resistance of the printed electric circuit was changed from 236 mΩ to 47 mΩ and the electric property was enhanced. It was found that the reduction of electric resistance was caused by surface property changes. Different surface analysis methods including scanning electron microscopy (SEM) and x-ray photoelectron spectroscopy (XPS) were used to understand the effectiveness of the proposed method. The results showed that the microsurface structure remained the same original structure before and after treatment. It was found that the surface carbon concentration was significantly increased after treatment. Detailed analysis showed that the C-C bond increased obviously after treatment. The change of electrical resistance was found to be limited to the material’s surface. After polishing, the circuit resistance was changed back to its original value. As the electric circuit is the basic element of electric devices, the proposed method enables the fabrication of high performance devices such as capacitors, strain gauge, and other sensors, which has potential applications in many areas such as industrial, aerospace, and military usage.
Effect of Strengthening Methods on Two-Way Slab under Low-Velocity Impact Loading
Authors: Yoo Sun-Jae , Yuan Tian-Feng , Hong Se-Hee , Yoon Young-Soo
Published: December 2020

In this study, the performance of reinforced concrete slabs strengthened using four methods was investigated under impact loads transferred from the top side to bottom side. The top and bottom sides of test slabs were strengthened by no-slump high-strength, high-ductility concrete (NSHSDC), fiber-reinforced-polymer (FRP) sheet, and sprayed FRP, respectively. The test results indicated that the test specimens strengthened with FRP series showed a 4% increase in reaction force and a decrease in deflection by more than 20% compared to the non-strengthened specimens. However, the specimen enhanced by the NSHSDC jacket at both the top and bottom sides exhibited the highest reaction force and energy dissipation as well as the above measurements because it contains two types of fibers in the NSHSDC. In addition, the weight loss rate was improved by approximately 0.12% for the NSHSDC specimen, which was the lowest among the specimens when measuring the weight before and after the impact load. Therefore, a linear relationship between the top and bottom strengthening of the NSHSDC and the impact resistance was confirmed, concluding that the NSHSDC is effective for impact resistance when the top and bottom sides are strengthened. The results of the analysis of the existing research show that the NSHSDC is considered to have high impact resistance, even though it has lower resistance than the steel fiber reinforced concrete and ultra-high-performance-concrete, it can be expected to further studies on strengthening of NSHSDC.
Effects of Intrabony Length and Cortical Bone Density on the Primary Stability of Orthodontic Miniscrews
Authors: Jin Jie , Kim Gi-Tae , Kwon Jae-Sung , Choi Sung-Hwan
Published: December 2020

Miniscrews have gained recent popularity as temporary anchorage devices in orthodontic treatments, where failure due to sinus perforations or damage to the neighboring roots have increased. Issues regarding miniscrews in insufficient interradicular space must also be resolved. This study aimed to evaluate the primary stability of miniscrews shorter than 6 mm and their feasibility in artificial bone with densities of 30, 40, and 50 pounds per cubic foot (pcf). The primary stability was evaluated by adjusting the intrabony miniscrew length, based on several physical properties: maximum insertion torque (MIT), maximum removal torque (MRT), removal angular momentum (RAM), horizontal resistance, and micromotion. The MIT and micromotion results demonstrated that the intrabony length of a miniscrew significantly affected its stability in low-density cortical bone, unlike cases with a higher cortical bone density (p < 0.05). The horizontal resistance, MRT, and RAM were affected by the intrabony length, regardless of the bone density (p < 0.05). Thus, the primary stability of miniscrews was affected by both the cortical bone density and intrabony length. The effect of the intrabony length was more significant in low-density cortical bone, where the implantation depth increased as more energy was required to remove the miniscrew. This facilitated higher resistance and a lower risk of falling out.
A Study on the Algorithm of Quality Evaluation for Fiber Laser Welding Process of ASTM A553-1 (9% Nickel Steel) Using Determination of Solidification Crack Susceptibility
Authors: Park Minho , Kim Jisun , Pyo Changmin , Son JoonSik , Kim Jaewoong
Published: December 2020

To prevent the contamination of the marine environment caused by ship exhaust gas, the demand for LNG (liquefied natural gas) fueled ships is increasing worldwide. A tank to store LNG at cryogenic temperatures is indispensable to such LNG-fueled ships. Since the materials used for LNG fuel propulsion tanks must have excellent mechanical properties such as impact toughness at cryogenic temperatures, the International Maritime Organization limits the IGC Code only to four types. Most of the tank materials for LNG-fueled ships ordered recently are adopting ASTM A553-1 material, but a systematic study to analyze the problem of quality degradation that may occur when welding A553-1 steel is required to secure the safety of cryogenic tanks. Therefore, in this study, among various quality problems, the tendency of weld solidification crack vulnerability is identified, and a decision system and optimization procedure are developed. In addition, a method of securing the welding quality of A553-1 steel was proposed by setting quality deterioration standards.
Mechanism Based Flow Stress Model for Alloy 625 and Alloy 718
Authors: Malmelöv Andreas , Fisk Martin , Lundbäck Andreas , Lindgren Lars-Erik
Published: December 2020

To predict the final geometry in thermo-mechanical processes, the use of modeling tools is of great importance. One important part of the modeling process is to describe the response correctly. A previously published mechanism-based flow stress model has been further developed and adapted for the nickel-based superalloys, alloy 625, and alloy 718. The updates include the implementation of a solid solution strengthening model and a model for high temperature plasticity. This type of material model is appropriate in simulations of manufacturing processes where the material undergoes large variations in strain rates and temperatures. The model also inherently captures stress relaxation. The flow stress model has been calibrated using compression strain rate data ranging from 0.01 to 1 s−1 with a temperature span from room temperature up to near the melting temperature. Deformation mechanism maps are also constructed which shows when the different mechanisms are dominating. After the model has been calibrated, it is validated using stress relaxation tests. From the parameter optimization, it is seen that many of the parameters are very similar for alloy 625 and alloy 718, although it is two different materials. The modeled and measured stress relaxation are in good agreement.
Magnetic Field Generated during Electric Current-Assisted Sintering: From Health and Safety Issues to Lorentz Force Effects
Authors: Deng Huaijiu , Dong Jian , Boi Filippo , Saunders Theo , Hu Chunfeng , Grasso Salvatore
Published: December 2020

In the past decade, a renewed interest on electromagnetic processing of materials has motivated several investigations on the interaction between matter, electric and magnetic fields. These effects are primarily reconducted to the Joule heating and very little attention has been dedicated to the magnetic field contributions. The magnetic field generated during electric current-assisted sintering has not been widely investigated. Magnetism could have significant effects on sintering as it generates significant magnetic forces, resulting in inductive electrical loads and preferential heating induced by overlapping magnetic fields (i.e., proximity effect). This work summarizes the magnetic field effects in electric current-assisted processing; it focuses on health and safety issues associated with large currents (up to 0.4 MA); using FEM simulations, it computes the self-generated magnetic field during spark plasma sintering (SPS) to consolidate materials with variable magnetic permeability; and it quantifies the Lorentz force acting at interparticle contact points. The results encourage one to pay more attention to magnetic field-related effects in order to engineer and exploit their potentials.
Sterically Hindered Phosphonium Salts: Structure, Properties and Palladium Nanoparticle Stabilization
Authors: Arkhipova Daria M. , Ermolaev Vadim V. , Miluykov Vasily A. , Gubaidullin Aidar T. , Islamov Daut R. , Kataeva Olga N. , Ananikov Valentine P.
Published: December 2020

A new family of sterically hindered alkyl(tri-tert-butyl) phosphonium salts (n-CnH2n+1 with n = 2, 4, 6, 8, 10, 12, 14, 16, 18, 20) was synthesized and evaluated as stabilizers for the formation of palladium nanoparticles (PdNPs), and the prepared PdNPs, stabilized by a series of phosphonium salts, were applied as catalysts of the Suzuki cross-coupling reaction. All investigated phosphonium salts were found to be excellent stabilizers of metal nanoparticles of small catalytically active size with a narrow size distribution. In addition, palladium nanoparticles exhibited exceptional stability: the presence of phosphonium salts prevented agglomeration and precipitation during the catalytic reaction.
Saturated Fatty Acids Promote GDF15 Expression in Human Macrophages through the PERK/eIF2/CHOP Signaling Pathway
Authors: L’homme Laurent , Sermikli Benan , Staels Bart , Piette Jacques , Legrand-Poels Sylvie , Dombrowicz David
Published: December 2020

Growth differentiation factor-15 (GDF-15) and its receptor GFRAL are both involved in the development of obesity and insulin resistance. Plasmatic GDF-15 level increases with obesity and is positively associated with disease progression. Despite macrophages have been recently suggested as a key source of GDF-15 in obesity, little is known about the regulation of GDF-15 in these cells. In the present work, we sought for potential pathophysiological activators of GDF15 expression in human macrophages and identified saturated fatty acids (SFAs) as strong inducers of GDF15 expression and secretion. SFAs increase GDF15 expression through the induction of an ER stress and the activation of the PERK/eIF2/CHOP signaling pathway in both PMA-differentiated THP-1 cells and in primary monocyte-derived macrophages. The transcription factor CHOP directly binds to the GDF15 promoter region and regulates GDF15 expression. Unlike SFAs, unsaturated fatty acids do not promote GDF15 expression and rather inhibit both SFA-induced GDF15 expression and ER stress. These results suggest that free fatty acids may be involved in the control of GDF-15 and provide new molecular insights about how diet and lipid metabolism may regulate the development of obesity and T2D.
Rhinacanthus nasutus “Tea” Infusions and the Medicinal Benefits of the Constituent Phytochemicals
Authors: Brimson James Michael , Prasanth Mani Iyer , Malar Dicson Sheeja , Brimson Sirikalaya , Tencomnao Tewin
Published: December 2020

Rhinacanthus nasutus (L.) Kurz (Acanthaceae) (Rn) is an herbaceous shrub native to Thailand and much of South and Southeast Asia. It has several synonyms and local or common names. The root of Rn is used in Thai traditional medicine to treat snake bites, and the roots and/or leaves can be made into a balm and applied to the skin for the treatment of skin infections such as ringworm, or they may be brewed to form an infusion for the treatment of inflammatory disorders. Rn leaves are available to the public for purchase in the form of “tea bags” as a natural herbal remedy for a long list of disorders, including diabetes, skin diseases (antifungal, ringworm, eczema, scurf, herpes), gastritis, raised blood pressure, improved blood circulation, early-stage tuberculosis antitumor activity, and as an antipyretic. There have been many studies investigating the roles of Rn or compounds isolated from the herb regarding diseases such as Alzheimer’s and other neurodegenerative diseases, cancer, diabetes and infection with bacteria, fungi or viruses. There have, however, been no clinical trials to confirm the efficacy of Rn in the treatment of any of these disorders, and the safety of these teas over long periods of consumption has never been tested. This review assesses the recent research into the role of Rn and its constituent compounds in a range of diseases.
Sports Chaplaincy, Theology and Social Theory Disrupting Performance-Based Identity in Elite Sporting Contexts
Authors: Jones Luke , Parker Andrew , Daniels Graham
Published: December 2020

Existing literature on the work of sports chaplains has focused primarily on practitioner accounts of chaplaincy with elite athletes. While these narratives provide useful descriptions of personal experience and practical application, they are largely devoid of theoretical grounding. This paper seeks to address this imbalance by proposing the need for sports chaplains to have a more critical understanding of sport and its relational dynamics. We begin by problematizing some of the historical assumptions underpinning elite sport, especially in relation to identity formation. We then explore some of the moral dilemmas which may be experienced by Christian athletes who inhabit contemporary sporting contexts. In line with the work of established sociological scholars, we then move to a critical analysis of performance-based identity and how an understanding of sociological concepts and ideas might assist chaplains in their work with elite athletes. The paper concludes by identifying sports chaplains as key figures in the disruption of performance-based identity.
The Converging of the Ways?—What Sabbath Practice Can Teach Us about Jewish-Christian and Intra-Religious Relations Today
Authors: Carter Erik C.
Published: December 2020

Given the tenuous relationship Christians have had with Jews over the centuries, not to mention division among Christianity on points of doctrine and practice, a contemporary examination of the Sabbath could be an opportunity to bring Jews and Christians into further dialogue with each other, not on the basis of a shared written text, but rather the living texts of religious experience. However, a review of the literature reveals a scarcity of empirical research on the Sabbath, especially how religious professionals practice Sabbath as exemplars in their spheres of influence. In this study, I, therefore, offer a comparative description of my findings with respect to two practical theological studies I conducted on Shabbat/Sabbath practice, one with American pulpit rabbis and the other Seventh-day Adventist pastors. As a practical theological project, I offer a theological reflection of the data, followed by implications for theological (re)construction and revised praxis for the Church and Jewish-Christian relations.
Enhancing Small Moving Target Detection Performance in Low-Quality and Long-Range Infrared Videos Using Optical Flow Techniques
Authors: Kwan Chiman , Budavari Bence
Published: December 2020

The detection of small moving objects in long-range infrared videos is challenging due to background clutter, air turbulence, and small target size. In this paper, we summarize the investigation of efficient ways to enhance the performance of small target detection in long-range and low-quality infrared videos containing moving objects. In particular, we focus on unsupervised, modular, flexible, and efficient methods for target detection performance enhancement using motion information extracted from optical flow methods. Three well-known optical flow methods were studied. It was found that optical flow methods need to be combined with contrast enhancement, connected component analysis, and target association in order to be effective for target detection. Extensive experiments using long-range mid-wave infrared (MWIR) videos from the Defense Systems Information Analysis Center (DSIAC) dataset clearly demonstrated the efficacy of our proposed approach.
An Edge-Sense Bidirectional Pyramid Network for Stereo Matching of VHR Remote Sensing Images
Authors: Tao Rongshu , Xiang Yuming , You Hongjian
Published: December 2020

As an essential step in 3D reconstruction, stereo matching still faces unignorable problems due to the high resolution and complex structures of remote sensing images. Especially in occluded areas of tall buildings and textureless areas of waters and woods, precise disparity estimation has become a difficult but important task. In this paper, we develop a novel edge-sense bidirectional pyramid stereo matching network to solve the aforementioned problems. The cost volume is constructed from negative to positive disparities since the disparity range in remote sensing images varies greatly and traditional deep learning networks only work well for positive disparities. Then, the occlusion-aware maps based on the forward-backward consistency assumption are applied to reduce the influence of the occluded area. Moreover, we design an edge-sense smoothness loss to improve the performance of textureless areas while maintaining the main structure. The proposed network is compared with two baselines. The experimental results show that our proposed method outperforms two methods, DenseMapNet and PSMNet, in terms of averaged endpoint error (EPE) and the fraction of erroneous pixels (D1), and the improvements in occluded and textureless areas are significant.
Assessing Community-Level Livability Using Combined Remote Sensing and Internet-Based Big Geospatial Data
Authors: Zhu Likai , Guo Yuanyuan , Zhang Chi , Meng Jijun , Ju Lei , Zhang Yuansuo , Tang Wenxue
Published: December 2020

With rapid urbanization, retrieving livability information of human settlements in time is essential for urban planning and governance. However, livability assessments are often limited by data availability and data update cycle, and this problem is more serious when making an assessment at finer spatial scales (e.g., community level). Here we aim to develop a reliable and dynamic model for community-level livability assessment taking Linyi city in Shandong Province, China as a case study. First, we constructed a hierarchical index system for livability assessment, and derived data for each index and community from remotely sensed data or Internet-based geospatial data. Next, we calculated the livability scores for all communities and assessed their uncertainties using Monte Carlo simulations. The results showed that the mean livability score of all communities was 59. The old urban and newly developed districts of our study area had the best livability, and got a livability score of 62 and 58 respectively, while industrial districts had the poorest conditions with an average livability score of 48. Results by dimension showed that the old urban district had better conditions of living amenity and travel convenience, but poorer conditions of environmental health and comfort. The newly developed districts were the opposite. We conclude that our model is effective and extendible for rapidly assessing community-level livability, which provides detailed and useful information of human settlements for sustainable urban planning and governance.
Vicarious Methodologies to Assess and Improve the Quality of the Optical Remote Sensing Images: A Critical Review
Authors: Kabir Sakib , Leigh Larry , Helder Dennis
Published: December 2020

Over the past decade, number of optical Earth-observing satellites performing remote sensing has increased substantially, dramatically increasing the capability to monitor the Earth. The quantity of remote sensing satellite increase is primarily driven by improved technology, miniaturization of components, reduced manufacturing, and launch cost. These satellites often lack on-board calibrators that a large satellite utilizes to ensure high quality (radiometric, geometric, spatial quality, etc.) scientific measurement. To address this issue, this work presents “best” vicarious image quality assessment and improvement techniques for those kinds of optical satellites which lack an on-board calibration system. In this article, image quality categories have been explored, and essential quality parameters (absolute and relative calibration, aliasing, etc.) have been identified. For each of the parameters, appropriate characterization methods are identified along with their specifications or requirements. In cases of multiple methods, recommendations have been made based-on the strengths and weaknesses of each method. Furthermore, processing steps have been presented, including examples. Essentially, this paper provides a comprehensive study of the criteria that need to be assessed to evaluate remote sensing satellite data quality, and the best vicarious methodologies to evaluate identified quality parameters such as coherent noise and ground sample distance.
Analysis of Drought Impact on Croplands from Global to Regional Scale: A Remote Sensing Approach
Authors: Ghazaryan Gohar , König Simon , Rezaei Ehsan , Siebert Stefan , Dubovyk Olena
Published: December 2020

Drought is one of the extreme climatic events that has a severe impact on crop production and food supply. Our main goal is to test the suitability of remote sensing-based indices to detect drought impacts on crop production from a global to regional scale. Moderate resolution imaging spectroradiometer (MODIS) based imagery, spanning from 2001 to 2017 was used for this task. This includes the normalized difference vegetation index (NDVI), land surface temperature (LST), and the evaporative stress index (ESI), which is based on the ratio of actual to potential evapotranspiration. These indices were used as indicators of drought-induced vegetation conditions for three main crops: maize, wheat, and soybean. The start and end of the growing season, as observed at 500 m resolution, were used to exclude the time steps that are outside of the growing season. Based on the three indicators, monthly standardized anomalies were estimated, which were used for both analyses of spatiotemporal patterns of drought and the relationship with yield anomalies. Anomalies in the ESI had higher correlations with maize and wheat yield anomalies than other indices, indicating that prolonged periods of low ESI during the growing season are highly correlated with reduced crop yields. All indices could identify past drought events, such as the drought in the USA in 2012, Eastern Africa in 2016–2017, and South Africa in 2015–2016. The results of this study highlight the potential of the use of moderate resolution remote sensing-based indicators combined with phenometrics for drought-induced crop impact monitoring. For several regions, droughts identified using the ESI and LST were more intense than the NDVI-based results. We showed that these indices are relevant for agricultural drought monitoring at both global and regional scales. They can be integrated into drought early warning systems, process-based crop models, as well as can be used for risk assessment and included in advanced decision-support frameworks.