bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a Novel Circulating Autoantibody Biomarker Panel for the Identification of Patients with ‘Actionable’ Pulmonary Nodules
Authors: Auger Claire , Moudgalya Hita , Neely Matthew R. , Stephan Jeremy T. , Tarhoni Imad , Gerard David , Basu Sanjib , Fhied Cristina L. , Abdelkader Ahmed , Vargas Moises , Hu Shaohui , Hulett Tyler , Liptay Michael J. , Shah Palmi , Seder Christopher W. , Borgia Jeffrey A.
Published: April 2023

Due to poor compliance and uptake of LDCT screening among high-risk populations, lung cancer is often diagnosed in advanced stages where treatment is rarely curative. Based upon the American College of Radiology’s Lung Imaging and Reporting Data System (Lung-RADS) 80–90% of patients screened will have clinically “non-actionable” nodules (Lung-RADS 1 or 2), and those harboring larger, clinically “actionable” nodules (Lung-RADS 3 or 4) have a significantly greater risk of lung cancer. The development of a companion diagnostic method capable of identifying patients likely to have a clinically actionable nodule identified during LDCT is anticipated to improve accessibility and uptake of the paradigm and improve early detection rates. Using protein microarrays, we identified 501 circulating targets with differential immunoreactivities against cohorts characterized as possessing either actionable (n = 42) or non-actionable (n = 20) solid pulmonary nodules, per Lung-RADS guidelines. Quantitative assays were assembled on the Luminex platform for the 26 most promising targets. These assays were used to measure serum autoantibody levels in 841 patients, consisting of benign (BN; n = 101), early-stage non-small cell lung cancer (NSCLC; n = 245), other early-stage malignancies within the lung (n = 29), and individuals meeting United States Preventative Screening Task Force (USPSTF) screening inclusion criteria with both actionable (n = 87) and non-actionable radiologic findings (n = 379). These 841 patients were randomly split into three cohorts: Training, Validation 1, and Validation 2. Of the 26 candidate biomarkers tested, 17 differentiated patients with actionable nodules from those with non-actionable nodules. A random forest model consisting of six autoantibody (Annexin 2, DCD, MID1IP1, PNMA1, TAF10, ZNF696) biomarkers was developed to optimize our classification performance; it possessed a positive predictive value (PPV) of 61.4%/61.0% and negative predictive value (NPV) of 95.7%/83.9% against Validation cohorts 1 and 2, respectively. This panel may improve patient selection methods for lung cancer screening, serving to greatly reduce the futile screening rate while also improving accessibility to the paradigm for underserved populations.
A Novel Preclinical Murine Model to Monitor Inflammatory Breast Cancer Tumor Growth and Lymphovascular Invasion
Authors: Rickard Ashlyn G. , Sannareddy Dorababu S. , Bennion Alexandra , Patel Pranalee , Sauer Scott J. , Rouse Douglas C. , Bouchal Samantha , Liu Harrison , Dewhirst Mark W. , Palmer Gregory M. , Devi Gayathri R.
Published: April 2023

Inflammatory breast cancer (IBC), an understudied and lethal breast cancer, is often misdiagnosed due to its unique presentation of diffuse tumor cell clusters in the skin and dermal lymphatics. Here, we describe a window chamber technique in combination with a novel transgenic mouse model that has red fluorescent lymphatics (ProxTom RFP Nu/Nu) to simulate IBC clinicopathological hallmarks. Various breast cancer cells stably transfected to express green or red fluorescent reporters were transplanted into mice bearing dorsal skinfold window chambers. Intravital fluorescence microscopy and the in vivo imaging system (IVIS) were used to serially quantify local tumor growth, motility, length density of lymph and blood vessels, and degree of tumor cell lymphatic invasion over 0–140 h. This short-term, longitudinal imaging time frame in studying transient or dynamic events of diffuse and collectively migrating tumor cells in the local environment and quantitative analysis of the tumor area, motility, and vessel characteristics can be expanded to investigate other cancer cell types exhibiting lymphovascular invasion, a key step in metastatic dissemination. It was found that these models were able to effectively track tumor cluster migration and dissemination, which is a hallmark of IBC clinically, and was recapitulated in these mouse models.
Current Progress on Predictive Biomarkers for Response to Immune Checkpoint Inhibitors in Gastric Cancer: How to Maximize the Immunotherapeutic Benefit?
Authors: Liu Yongqing , Hu Pengbo , Xu Liang , Zhang Xiuyuan , Li Zhou , Li Yiming , Qiu Hong
Published: April 2023

Gastric cancer is the fifth most prevalent cancer and the fourth leading cause of cancer death globally. Delayed diagnosis and pronounced histological and molecular variations increase the complexity and challenge of treatment. Pharmacotherapy, which for a long time was systemic chemotherapy based on 5-fluorouracil, is the mainstay of management for advanced gastric cancer. Trastuzumab and programmed cell death 1 (PD-1) inhibitors have altered the therapeutic landscape, contributing to noticeably prolonged survivorship in patients with metastatic gastric cancer. However, research has revealed that immunotherapy is only beneficial to some individuals. Biomarkers, such as programmed cell death ligand 1 (PD-L1), microsatellite instability (MSI), and tumor mutational load (TMB), have been shown to correlate with immune efficacy in numerous studies and are increasingly employed for the selection of patients most likely to respond to immunotherapy. Gut microorganisms, genetic mutations like POLE/POLD1 and NOTCH4, tumor lymphoid infiltrating cells (TILs), and other novel biomarkers have the potential to develop into new predictors. Prospective immunotherapy for gastric cancer should be guided by a biomarker-driven precision management paradigm, and multidimensional or dynamic marker testing could be the way to go.
Interaction between Radiation Therapy and Targeted Therapies in HER2-Positive Breast Cancer: Literature Review, Levels of Evidence for Safety and Recommendations for Optimal Treatment Sequence
Authors: Debbi Kamel , Grellier Noémie , Loganadane Gokoulakrichenane , Boukhobza Chahrazed , Mahé Mathilde , Cherif Mohamed Aziz , Rida Hanan , Gligorov Joseph , Belkacemi Yazid
Published: April 2023

Purpose: Over the past twenty years, anti-HER2 targeted therapies have proven to be a revolution in the management of human epidermal growth receptor 2 (HER2)-positive breast cancers. Anti-HER2 therapies administered alone or in combination with chemotherapy have been specifically studied. Unfortunately, the safety of anti-HER2 therapies in combination with radiation remains largely unknown. Thus, we propose a literature review of the risks and safety of combining radiotherapy with anti-HER2 therapies. We will focus on the benefit/risk rationale and try to understand the risk of toxicity in early-stage and advanced breast cancer. Methods: Research was carried out on the following databases: PubMed, EMBASE, ClinicalTrial.gov, Medline, and Web of Science for the terms “radiotherapy”, “radiation therapy”, “radiosurgery”, “local ablative therapy”, and “stereotactic”, combined with “trastuzumab”, “pertuzumab”, “trastuzumab emtansine”, “TDM-1”, “T-Dxd”, “trastuzumab deruxtecan”, “tucatinib”, “lapatinib”, “immune checkpoint inhibitors”, “atezolizumab”, “pembrolizumab”, “nivolumab”, “E75 vaccine”, “interferon”, “anti-IL-2”, “anti-IL 12”, and “ADC”. Results: Association of radiation and monoclonal antibodies such as trastuzumab and pertuzumab (with limited data) seems to be safe, with no excess risk of toxicity. Preliminary data with radiation and of antibody–drug conjugate of trastuzumab combined cytotoxic (trastuzumab emtansine, trastuzumab deruxtecan), given the underlying mechanism of action, suggest that one must be particularly cautious with the association. The safety of the combination of a tyrosine kinase inhibitor (lapatinib, tucatinib) and radiation remains under-studied. The available evidence suggests that checkpoint inhibitors can be safely administrated with radiation. Conclusions: HER2-targeting monoclonal antibodies and checkpoint inhibitors can be combined with radiation, apparently with no excess toxicities. Caution is required when associating radiation with TKI and antibody drugs, considering the limited evidence.
Recent Advances on Small Band Gap Semiconductor Materials (≤2.1 eV) for Solar Water Splitting
Authors: Zhang Hefeng , Liu Jiaqi , Xu Ting , Ji Wenqian , Zong Xu
Published: April 2023

The conversion of solar energy into renewable H2 fuel via photoelectrochemical and photocatalytic water splitting approaches has attracted considerable attention due to its potential to solve significant energy and environmental issues. To achieve reasonable energy conversion efficiency of 10%, which is amenable to the economic feasibility of this technology, semiconductor materials, which are the main carrier for solar H2 production, must fulfill several important criteria. One of the most important criteria is the band gap of the semiconductor material, which should be low enough to allow the efficient absorption of incident light. This is because the band gap of semiconductor material fundamentally determines the upper limit of the theoretical energy conversion efficiency of the solar conversion system. In this work, recent advances in utilizing semiconductor material with a band gap smaller than 2.1 eV instead of the simply-defined visible-light-responsive semiconductor materials toward solar H2 production from water splitting was reviewed.
Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells
Authors: Baik June , Ortiz-Cordero Carolina , Magli Alessandro , Azzag Karim , Crist Sarah B. , Yamashita Aline , Kiley James , Selvaraj Sridhar , Mondragon-Gonzalez Ricardo , Perrin Elizabeth , Maufort John P. , Janecek Jody L. , Lee Rachael M. , Stone Laura Hocum , Rangarajan Parthasarathy , Ramachandran Sabarinathan , Graham Melanie L. , Perlingeiro Rita C. R.
Published: April 2023

Pluripotent stem (PS) cells enable the scalable production of tissue-specific derivatives with therapeutic potential for various clinical applications, including muscular dystrophies. Given the similarity to human counterparts, the non-human primate (NHP) is an ideal preclinical model to evaluate several questions, including delivery, biodistribution, and immune response. While the generation of human-induced PS (iPS)-cell-derived myogenic progenitors is well established, there have been no data for NHP counterparts, probably due to the lack of an efficient system to differentiate NHP iPS cells towards the skeletal muscle lineage. Here, we report the generation of three independent Macaca fascicularis iPS cell lines and their myogenic differentiation using PAX7 conditional expression. The whole-transcriptome analysis confirmed the successful sequential induction of mesoderm, paraxial mesoderm, and myogenic lineages. NHP myogenic progenitors efficiently gave rise to myotubes under appropriate in vitro differentiation conditions and engrafted in vivo into the TA muscles of NSG and FKRP-NSG mice. Lastly, we explored the preclinical potential of these NHP myogenic progenitors in a single wild-type NHP recipient, demonstrating engraftment and characterizing the interaction with the host immune response. These studies establish an NHP model system through which iPS-cell-derived myogenic progenitors can be studied.
Buckling Characteristics of Different Cross-Sectioned LGFR-PP Stiffeners under Axial Compression
Authors: Nie Jin , Gao Wenbo , Li Guibing
Published: April 2023

Long glass fiber-reinforced polypropylene (LGFR-PP) composite structures with stiffeners are important substitutes for metal parts for vehicle lightweighting; a good understanding of the buckling characteristics of LGFR-PP stiffeners would provide an important reference for engineering design. The current work is therefore intended to study the buckling characteristics of different cross-sectioned LGFR-PP stiffeners under axial compression via experimental and theoretical analysis. Firstly, LGFR-PP stiffeners with semicircular, rectangular, and trapeziform cross-sections were compressed at the axial direction using a universal testing machine to obtain the buckling process data. Then, the elasticity stability theory modified according to the experimental results was derived to estimate the buckling resistance of LGFR-PP stiffeners in different designs. The test results showed that the LGFR-PP stiffeners possessed a flexible–torsional bulking instability mode under axial compression, the LGFR-PP stiffeners with a semicircular cross-section had higher compression buckling resistance, and the rectangular and trapeziform cross-sectioned stiffeners had better rigidity. The theoretical analysis showed that the modified elasticity stability theory could generally predict the buckling resistance of LGFR-PP stiffeners under axial compression.
X-ray Diffraction and Trace Element Analyses of K/Pg Boundary Samples Collected from Agost and Caravaca, Spain
Authors: Li Zhaohui , Hong Hanlie , Liao Libing , He Hongping
Published: April 2023

The boundary between Cretaceous and Paleogene (K/Pg) plays an important role in deciphering the Earth’s history and biological evolution from Mesozoic to Cenozoic. As such, the delineation and characterization of the boundary layer has attracted significant attention. In this study, X-ray diffraction (XRD) and elemental analyses were conducted to characterize the samples of boundary layer and the layers around Agost and Caravaca, Spain. The XRD results showed that the layers immediately above and below the boundary layer are made of limestone, while in the boundary layer, a significant increase in the clay minerals smectite, kaolinite, and illite was observed. The major element analyses revealed an increase in Si and Al contents, confirming the presence of clay minerals. The trace element analyses showed elevated concentrations of V, Cr, Ni, Zn, Pb, and Th, but not for Rb, Cu, and U. The rare Earth element (REE) analyses showed elevated La, Ce, and Nd concentrations in the boundary layer. Correlation analyses between selected trace elements and REE showed good agreements, with R2 values of about 0.9. The results agreed well with the finding in the area, except the lower contents of Rb, Cu, and U; thus, they may promote further studies to make detailed comparisons.
Marked Range Regression and Possible Alteration of Distribution of the Dupont’s Lark Chersophilus duponti in Tunisia: Conservation Consequences of Vanishing Alfa Grass Stipa tenacissima Steppes in North Africa
Authors: Viñuela Javier , García Jesus T. , Suárez Francisco
Published: April 2023

The effect of global warming and desertification on bird populations of semi-arid North African ecosystems has been little studied, although ecosystems in those areas are suffering dramatic changes. Dupont’s lark is one of the most endangered passerines in Europe, but it is also considered scarce in North Africa, where its range and numbers are only well known for Morocco. We analyzed the current distribution and population size of Dupont’s lark in Tunisia and compared the current figures with older data. To assess the presence of the species in the patches of adequate habitat that we found, we elicited territorial calls by broadcasting the males’ territorial songs during early breeding season (N = 123, ≈40 h). Fieldwork (45 persons/day) and analysis of satellite images were combined to determine the current minimal extent of occurrence and area of occupancy, following IUCN definitions. In the only habitat where we found the species (well-preserved pure or mixed alfa patches in the Feriana-Kasserine region), the extent of occurrence in and effective area of occupancy were small (56.3 and 33.2 km2, respectively), particularly when compared with previous estimates (presence of the species in adequate habitat over ca. 89,000 km2). The species has not been detected at all in a large area in the southern part of its potential range, where additional surveys are urgently needed to locate possible remnant small and isolated populations that could persist, as suggested by two recent records of the species there. The breeding population of Dupont’s lark in Tunisia is estimated to be fewer than 600 song birds (335–577). The drastic reduction of range and numbers of this species must have been caused by the disappearance or degradation of alfa grasslands due to agricultural development, overgrazing, and increased aridity.
An Operational Checklist of the Birds of Northwestern Italy (Piedmont and Aosta Valley)
Authors: Boano Giovanni , Pavia Marco , Alessandria Gianfranco , Mingozzi Toni
Published: April 2023

This paper provides the fourth edition of the checklist of birds recorded in northwestern Italy (the Piedmont and Aosta Valley regions) and covers more than 300 years of ornithological data, including subspecies. This work updates the previous works published in 1981, 2003, and 2009, with the revision of the AERC codes and the addition of special annotations for several species. We also provide some new settings to make the bird checklist a more useful tool for all users, particularly scholars and professionals interested in biodiversity assessment and conservation reports. To this end, (a) new coding concerning population estimates and trends, as well as risk categories (Red List), is introduced; (b) bird lists are structured for analysis at two temporal levels: the General Checklist (GCL), covering the period from 1685 to 2022, and the Operational Checklist (OCL), covering the decade 2010–2019, providing periods of reference for comparison and analysis; and (c) an electronic spreadsheet is provided as part of the online Supplementary Materials to allow for further data analysis by readers, if necessary. The list presently contains 408 species and 444 taxonomic units, which consist of both subspecies and monotypic species. Each of them has been allocated to one of the AERC categories A, B, C, or D, while category E has been excluded. Since the publication of the previous list (2009), 19 species have been added. The avifauna currently breeding in the Piedmont and Aosta Valley regions comprises 197 species, with an additional 9 species that were once breeders but are now considered regionally extinct.
Exploring the Perception of Dental Undergraduate Students and Faculty on Environmental Sustainability in Dentistry: A Cross-Sectional Survey in 26 Dental Schools in Saudi Arabia
Authors: Jamal Hasan , Marghalani Abdullah A. , Al-Sharif Ahmed , Shinawi Albatool , Gaffar Balgis , Al-Edaili Ebtsam Abdullah , Al-Baqami Ghaliah , AlQarni Mayson
Published: April 2023

There are few published studies assessing dental students’ and faculty’s perception regarding environmental sustainability in dentistry (ESD) and its existence within the dental curricula along with barriers and enablers for its integration. As far as the authors know, no published study has looked into this in Saudi Arabia, and this is what the current study aims to explore. A cross-sectional survey using validated online questionnaires for dental students and faculty was carried out in 26 dental schools in Saudi Arabia. The validated questionnaire utilized 25 questions using Qualtrics (XM) software. Both descriptive statistics and thematic analysis were included in the data analysis. ESD content is not formally embedded within the dental curricula in all 26 included universities in Saudi Arabia. Although the majority of students and faculty members agreed or strongly agreed on the importance of ESD as a whole and the importance of teaching it, more than 82% and 81% of students and faculty, respectively, demonstrated a lack of basic knowledge concerning ESD. The preliminary findings of the current study suggest that there is a high demand for incorporating ESD content in dental education in Saudi Arabia. To achieve this, resources and policy changes are necessary. A top-down approach is needed, including incorporating ESD into dental education standards, using evidence-based practices in revising infection control regulations, and providing institutional support through training, materials, and incentives. National dental associations and governments should provide practical solutions and actively support dental professionals in implementing ESD.
A High-Precision Calculation Method for Troposcatter Propagation Delay by Fusing Atmospheric Reanalysis Data
Authors: Sun Haoran , Liu Qiang , Zhang Shuang , Zhao Yu
Published: April 2023

Troposcatter propagation delay is one of the most significant sources of errors in troposcatter time comparison. The existing methods of calculating troposcatter propagation delay face problems in terms of effectively reflecting the influence of the meteorological environment on troposcatter propagation delay, due to the insufficient spatial and temporal resolution of the meteorological data. This article proposes a high-precision calculation method for troposcatter propagation delay based on atmospheric reanalysis data. The troposcatter propagation path and the refractive index along the troposcatter data were obtained by combining 3D ray tracing with the European Centre for Medium-Range Weather Forecasts’ Reanalysis 5 (ERA5), with high spatial and temporal resolution. This found the hour-level time delay in troposcatter. The geometric delay, path delay, and total propagation delay of the troposcatter were calculated and analyzed via 12 scattering links in 6 typical geographical regions. It was found that the path delay was the main cause of the propagation delay of troposcatter, and that the proportion of geometric delay in the total propagation delay increased along with an increase in link distance. The propagation delay changed noticeably in different seasons. The path delay was higher in summer and lower in winter, the geometric delay was lower in summer and higher in winter, and the total propagation delay was mainly high in summer and low in winter. There were differences in delay date, seasonal fluctuation amplitude, and in hourly fluctuation standard deviation among different geographic areas.
Agentless Approach for Security Information and Event Management in Industrial IoT
Authors: Zahid Huma , Hina Sadaf , Hayat Muhammad Faisal , Shah Ghalib A.
Published: April 2023

The Internet of Things (IoT) provides ease of real-time communication in homes, industries, health care, and many other dependable and interconnected sectors. However, in recent years, smart infrastructure, including cyber-physical industries, has witnessed a severe disruption of operation due to privilege escalation, exploitation of misconfigurations, firmware hijacking, malicious node injection, botnets, and other malware infiltrations. The proposed agentless module for Wazuh security information and event management (SIEM) solution contributes to securing small- to large-scale IoT networks of industry 4.0. An agentless module is implemented by vigilantly examining the IoT device traffic without installing any agent or software on the endpoints. In the proposed research scheme, a module sniffs the network traffic of IoT devices captured from the gateway and passes it to a machine learning model for initial detection and prediction. The output of the ML model is embedded in the JSON log format and passed through the Wazuh agent to the Wazuh server where a decoder is added that decodes the network traffic logs. For event monitoring in Wazuh, industrial protocols are also thoroughly analyzed, and the feature set is determined. These features are used to write rules which are tested on the SWaT dataset, utilizing a common industrial protocol (CIP) for communication. Custom and dynamic rules are written at the Wazuh end to generate alerts to respond to any anomaly detected by the machine learning (ML) model or in the protocols used. Finally, in case of any event or an attack is detected, the alerts are fired on the Wazuh dashboard. This agentless SIEM solution has practical implications for the security of the industrial control systems of industry 4.0.
A Novel Methodology for Strengthening Stability in Electrical Power Systems by Considering Fast Voltage Stability Index under N − 1 Scenarios
Authors: Jaramillo Manuel Dario , Carrión Diego Francisco , Muñoz Jorge Paul
Published: April 2023

An electrical power system (EPS) is subject to unexpected events that might cause the outage of elements such as transformers, generators, and transmission lines. For this reason, the EPS should be able to withstand the failure of one of these elements without changing its operational characteristics; this operativity functionality is called N−1 contingency. This paper proposes a methodology for the optimal location and sizing of a parallel static Var compensator (SVC) in an EPS to reestablish the stability conditions of the system before N−1 contingencies take place. The system’s stability is analyzed using the fast voltage stability index (FVSI) criterion, and the optimal SVC is determined by also considering the lowest possible cost. This research considers N−1 contingencies involving the disconnection of transmission lines. Then, the methodology analyzes every scenario in which a transmission line is disconnected. For every one of them, the algorithm finds the weakest transmission line by comparing FVSI values (the higher the FVSI, the closer the transmission line is to instability); afterward, when the weakest line is selected, by brute force, an SVC with values of 5 Mvar to 100 Mvars in steps of 5 Mvar is applied to the sending bus bar of this transmission line. Then, the SVC value capable of reestablishing each line’s FVSI to its pre-contingency value while also reestablishing each bus-bar’s voltage profile and having the lowest cost is selected as the optimal solution. The proposed methodology was tested on IEEE 14, 30, and 118 bus bars as case studies and was capable of reestablishing the FVSI in each contingency to its value prior to the outage, which indicates that the algorithm performs with 100% accuracy. Additionally, voltage profiles were also reestablished to their pre-contingency values, and in some cases, they were even higher than the original values. Finally, these results were achieved with a single solution for a unique SVC located in one bus bar that is capable of reestablishing operational conditions under all possible contingency scenarios.
Energy Management in a Standalone Microgrid: A Split-Horizon Dual-Stage Dispatch Strategy
Authors: Amir Aslam , Shareef Hussain , Awwad Falah
Published: April 2023

Microgrid technology has recently gained global attention over increasing demands for the inclusion of renewable energy resources in power grids, requiring constant research and development in aspects such as control, protection, reliability, and management. With an ever-increasing scope for maximizing renewable energy output, there is also a need to reduce the curtailment of power on both the generation and demand sides by increasing forecasting accuracies and using resources more effectively. This paper proposes a dual-stage dispatch employing a novel “split-horizon” strategy, in a bid to enhance energy management in a standalone microgrid. The split-horizon is essentially the considered time horizon split into equal operational periods of the dual-stage dispatch. The proposed strategy utilizes a custom-designed novel variant of the inertia-weight-based particle swarm optimization (PSO), termed customized PSO, to perform the optimal schedule and dispatch operation by benefitting from the simplicity of PSO and customization as per the considered objectives. A modified IEEE 34-node test system is derived into a standalone microgrid with added distributed energy resources to test the proposed strategy, while another standalone microgrid, a modified IEEE 69-node test feeder, is also considered for scalability. Furthermore, the validation of the strategy is performed appropriately with a case study while also validating the proposed optimization algorithm. It is observed that the proposed energy management strategy provides approximatelya 7% reduction in costs.
Is the Relationship between Corporate Social Responsibility, Environment and Energy Sufficiently Debated Nowadays?
Authors: Badareu Gabriela , Doran Nicoleta , Puiu Silvia , Bădîrcea Roxana , Manta Alina
Published: April 2023

The aim of this review was to analyze to what extent the relationship between corporate social responsibility (CSR), environment and energy has been addressed in the specialized literature and which are the deficient segments in this regard. VOSviewer was used for data extraction, mapping and grouping the articles from journals that have been indexed in Web of Science (WOS). A total of 102 papers were found approaching the topic of corporate social responsibility, environment and energy policy, 5192 research papers for CSR and the environment and 320 on CSR and energy policy. Following the bibliometric analysis, we can conclude that we are facing a shortage of works that analyze the relationship between the three fields together. The number of published articles was reduced to establish the direction of influence between the three variables, as well as the impact between them. However, the advantage that emerges from this lack of works is that there are many opportunities to find news on this subject and to discover solutions to improve the quality of life. This study can be used as an impetus for researchers to examine the trends of this research topic.
Review of Policies for Indonesia’s Electricity Sector Transition and Qualitative Evaluation of Impacts and Influences Using a Conceptual Dynamic Model
Authors: Zahari Teuku Naraski , McLellan Benjamin C.
Published: April 2023

Indonesia’s final energy demand is projected to increase by 70% in the next decade, with electricity expected to account for 32%. The increasing electricity demand poses a potential threat to national emissions reduction targets since fossil fuels generated 86% of the electricity in 2018, associated to 50% of the national CO2 emissions. Indonesia plans to reduce its CO2 emissions by 29% by increasing the total electricity generated from renewables, using a set of market-based and regulatory policies. However, economic, social, and environmental issues may arise from the widespread adoption of renewable energy. This study explores the economic, social, and environmental effects of renewable energy policies in the electricity sector. Our work presents an advance over previous studies that attempted to understand the electricity sector energy transition from a system perspective by exploring the structural feedback between it and economic, energy, and environmental systems. This enables the assessment of different energy policies using more macro indicators, which further emphasize the novelty of our work. A combination of system dynamics modelling and a policy analysis framework was applied to explore these issues. Our study proposes a dynamic hypothesis that the price of energy increases over time, in the absence of substitution, becoming a limiting factor in the transition to renewables in the electricity sector. The fiscal budget was found to be a bottleneck for renewable energy adoption in the electricity sector in Indonesia. We found that a fossil fuel depletion premium could be a potential supporting policy to enable the smooth phasing-out of fossil fuels and support a sustainable energy transition.
Pore Structure Characteristics and Influencing Factors of Tight Reservoirs Controlled by Different Provenance Systems: A Case Study of the Chang 7 Members in Heshui and Xin’anbian of the Ordos Basin
Authors: Xiao Ling , Bi Ling , Yi Tao , Lei Yutian , Wei Qinlian
Published: April 2023

The evaluation of pore structure is critical in understanding reservoir characteristics. This study aims to analyze the microscopic pore structure characteristics of tight reservoirs from various provenances through physical property analysis, casting thin sections, scanning electron microscopy (SEM), and constant−rate mercury injection. The pore throat parameters of the Chang 7 reservoir were analyzed and compared to those of the Xin’anbian and the Heshui areas. The results show that intergranular pores dominate the pore type of the Chang 7 reservoir in the Xin’anbian area, followed by feldspathic dissolution pores, with high-necked and tubular throat content caused by weak compaction and dissolution, respectively. On the other hand, feldspar dissolution pores dominate the pore type of the Chang 7 reservoir in the Heshui area, followed by intergranular pores, with a high content of tube−bundle throats caused by dissolution and flake−bent flake throats caused by compaction. The difference in pore parameters between the two blocks is minimal, but the difference in throat parameters is significant. Under the same permeability conditions, the throat radius distribution range of the Chang 7 reservoir samples in the Heshui area is narrower than that in the Xin’anbian area, with tiny throats contributing significantly to reservoir permeability. Sedimentary hydrodynamic conditions and diagenesis are the primary reasons for the differences in the pores, throats, and pore structure parameters of the Chang 7 tight reservoirs.
Decision Support for Defining Adaptive Façade Design Goals in the Early Design Phase
Authors: Voigt Michael P. , Roth Daniel , Kreimeyer Matthias
Published: April 2023

Compared to conventional façades, adaptive façades (AFs) can adjust their properties in response to environmental changes and user requirements. Often performed through the integration of actuators, sensors, and control units, this provides benefits such as reduced energy consumption in buildings but also increases the complexity of the façade design. To efficiently deal with the higher complexity, this article aims to provide suitable decision support for the early design phase, identify suitable design goals, and compare these to previously implemented Afs (make-or-buy decision). There is particular focus on the AF-specific characteristics, as these are new compared to well-known conventional façades. To systematically develop decision support, requirements are identified in expert interviews and the literature, and the current state of the art is evaluated against these. Research gaps found in current methods are addressed in this article, and continuous decision support is developed for the early design phase of an integrated design process. This support includes a checklist with AF-specific characteristics and a digitally implemented database of AFs. Based on the requirements, an evaluation is performed for both methods: this includes the comparison of the results to three ongoing AF projects and the assignment of 40 case studies to the database.
Impact of Ultrasonication on African Oil Bean (Pentaclethra macrophylla Benth) Protein Extraction and Properties
Authors: Nwokocha Blessing C. , Chatzifragkou Afroditi , Fagan Colette C.
Published: April 2023

African oil bean (Pentaclethra macrophylla Benth) is an underutilised edible oil seed that could represent a sustainable protein source. In this study, the impact of ultrasonication on the extraction efficiency and properties of protein from African oil bean (AOB) seeds was evaluated. The increase in the duration of extraction favoured the extraction of AOB proteins. This was observed by an increase in extraction yield from 24% to 42% (w/w) when the extraction time was increased from 15 min to 60 min. Desirable properties were observed in extracted AOB proteins; the amino acid profile of protein isolates revealed higher ratios of hydrophobic to hydrophilic amino acids compared to those of the defatted seeds, suggesting alterations in their functional properties. This was also supported by the higher proportion of hydrophobic amino acids and high surface hydrophobicity index value (3813) in AOB protein isolates. The foaming capacity of AOB proteins was above 200%, with an average foaming stability of 92%. The results indicate that AOB protein isolates can be considered promising food ingredients and could help stimulate the growth of the food industry in tropical Sub-Saharan regions where AOB seeds thrive.
Performance of a Handheld Near-Infrared Spectroscopy Device to Predict Pork Primal Belly Fat Iodine Value and Loin Lean Intramuscular Fat Content
Authors: Lam Stephanie , Rolland David , Zawadski Sophie , Wei Xinyi , Uttaro Bethany , Juárez Manuel
Published: April 2023

The increase in market demand and economic value of Canadian pork primal cuts has led to a need to assess advanced technologies capable of measuring quality traits. Fat and lean composition were measured using a Tellspec near-infrared (NIR) spectroscopy device to predict the pork belly fat iodine value (IV) and loin lean intramuscular fat (IMF) content in 158 pork belly primals and 419 loin chops. The calibration model revealed a 90.6% and 88.9% accuracy for the Tellspec NIR to predict saturated fatty acids (SFA) and IV, respectively, in the belly fat. The calibration model accuracy for the other belly fatty acids revealed an accuracy of 66.3–86.1%. Using the Tellspec NIR to predict loin lean IMF reported a lower accuracy for moisture (R2 = 60) and fat % (R2 = 40.4). This suggests that Tellspec NIR spectroscopy measures on the pork belly primal offers a cost-efficient, rapid, accurate, and non-invasive indicator of pork belly IV and could be used for the classification for specific markets.
Synthesis and Characterization of Biodegradable Poly(vinyl alcohol)-Chitosan/Cellulose Hydrogel Beads for Efficient Removal of Pb(II), Cd(II), Zn(II), and Co(II) from Water
Authors: Aljar Mona A. Aziz , Rashdan Suad , Almutawah Abdulla , El-Fattah Ahmed Abd
Published: April 2023

Globally, water contamination by heavy metals is a serious problem that affects the environment and human health. Adsorption is the most efficient way of water treatment for eliminating heavy metals. Various hydrogels have been prepared and used as adsorbents to remove heavy metals. By taking advantage of poly(vinyl alcohol) (PVA), chitosan (CS), cellulose (CE), and the process for physical crosslinking, we propose a simple method to prepare a PVA-CS/CE composite hydrogel adsorbent for the removal of Pb(II), Cd(II), Zn(II) and Co(II) from water. Structural analyses of the adsorbent were examined by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy-energy dispersive X-ray (SEM-EDX) analysis, and X-ray diffraction (XRD). PVA-CS/CE hydrogel beads had a good spherical shape together with a robust structure and suitable functional groups for the adsorption of heavy metals. The effects of adsorption parameters such as pH, contact time, adsorbent dose, initial concentration of metal ions, and temperature on the adsorption capacity of PVA-CS/CE adsorbent were studied. The adsorption characteristics of PVA-CS/CE for heavy metals may be completely explained by pseudo-second-order adsorption and the Langmuir adsorption model. The removal efficiency of PVA-CS/CE adsorbent for Pb(II), Cd(II), Zn(II), and Co(II) was 99, 95, 92, and 84%, respectively, within 60 min. The heavy metal’s hydrated ionic radius may be crucial in determining the adsorption preference. After five consecutive adsorption–desorption cycles, the removal efficiency remained over 80%. As a result, the outstanding adsorption-desorption properties of PVA-CS/CE can potentially be extended to industrial wastewater for heavy metal ion removal.
Nanoarchitectonics of a Skin-Adhesive Hydrogel Based on the Gelatin Resuscitation Fluid Gelatinol®
Authors: Osetrov Konstantin , Uspenskaya Mayya , Zaripova Faliya , Olekhnovich Roman
Published: April 2023

Hydrogel materials are one of the most versatile representatives of biomaterials. Their widespread use in medical practice is due to their similarity to native biostructures regarding relevant properties. This article discusses the synthesis of hydrogels based on a plasma-substituting Gelatinol® solution and modified tannin, carried out by direct mixing of the two solutions and brief heating. This approach makes it possible to obtain materials based on precursors that are safe for humans, while having antibacterial activity and high adhesion to human skin. Thanks to the synthesis scheme used, it is possible to obtain hydrogels with a complex shape before use, which is relevant in cases where industrial hydrogels do not fully satisfy the end use regarding their form factor. Using IR spectroscopy and thermal analysis, the distinctive aspects of mesh formation were shown in comparison with the hydrogels based on ordinary gelatin. A number of application properties, such as the physical and mechanical characteristics, permeability to oxygen/moisture, and antibacterial effect, were also considered. The sorption parameters of the material were characterized in a set of physiological buffers (pH 2–9) using Fick’s first law and a pseudo-second order equation. The adhesive shear strength was determined in a model system. The synthesized hydrogels showed potential for the further development of materials based on plasma-substituting solutions.
Epigenetic Alterations of Brain Non-Neuronal Cells in Major Mental Diseases
Authors: Abdolmaleky Hamid Mostafavi , Martin Marian , Zhou Jin-Rong , Thiagalingam Sam
Published: April 2023

The tissue-specific expression and epigenetic dysregulation of many genes in cells derived from the postmortem brains of patients have been reported to provide a fundamental biological framework for major mental diseases such as autism, schizophrenia, bipolar disorder, and major depression. However, until recently, the impact of non-neuronal brain cells, which arises due to cell-type-specific alterations, has not been adequately scrutinized; this is because of the absence of techniques that directly evaluate their functionality. With the emergence of single-cell technologies, such as RNA sequencing (RNA-seq) and other novel techniques, various studies have now started to uncover the cell-type-specific expression and DNA methylation regulation of many genes (e.g., TREM2, MECP2, SLC1A2, TGFB2, NTRK2, S100B, KCNJ10, and HMGB1, and several complement genes such as C1q, C3, C3R, and C4) in the non-neuronal brain cells involved in the pathogenesis of mental diseases. Additionally, several lines of experimental evidence indicate that inflammation and inflammation-induced oxidative stress, as well as many insidious/latent infectious elements including the gut microbiome, alter the expression status and the epigenetic landscapes of brain non-neuronal cells. Here, we present supporting evidence highlighting the importance of the contribution of the brain’s non-neuronal cells (in particular, microglia and different types of astrocytes) in the pathogenesis of mental diseases. Furthermore, we also address the potential impacts of the gut microbiome in the dysfunction of enteric and brain glia, as well as astrocytes, which, in turn, may affect neuronal functions in mental disorders. Finally, we present evidence that supports that microbiota transplantations from the affected individuals or mice provoke the corresponding disease-like behavior in the recipient mice, while specific bacterial species may have beneficial effects.
Biotransformation of High Concentrations of Ginsenoside Substrate into Compound K by β-glycosidase from Sulfolobus solfataricus
Authors: Wang Pan , Tang Congcong , Liu Yannan , Yang Jing , Fan Daidi
Published: April 2023

The rare ginsenoside Compound K (CK) is an attractive ingredient in traditional medicines, cosmetics, and the food industry because of its various biological activities. However, it does not exist in nature. The commonly used method for the production of CK is enzymatic conversion. In order to further improve the catalytic efficiency and increase the CK content, a thermostable β-glycosidase from Sulfolobus solfataricus was successfully expressed in Pichia pastoris and secreted into fermentation broth. The recombinant SS-bgly in the supernatant showed enzyme activity of 93.96 U/mg at 120 h when using pNPG as substrate. The biotransformation conditions were optimized at pH 6.0 and 80 °C, and its activity was significantly enhanced in the presence of 3 mM Li+. When the substrate concentration was 10 mg/mL, the recombinant SS-bgly completely converted the ginsenoside substrate to CK with a productivity of 507.06 μM/h. Moreover, the recombinant SS-bgly exhibited extraordinary tolerance against high substrate concentrations. When the ginsenoside substrate concentration was increased to 30 mg/mL, the conversion could still reach 82.5% with a productivity of 314.07 μM/h. Thus, the high temperature tolerance, resistance to a variety of metals, and strong substrate tolerance make the recombinant SS-bgly expressed in P. pastoris a potential candidate for the industrial production of the rare ginsenoside CK.
Mapping the Genomic Regions Controlling Germination Rate and Early Seedling Growth Parameters in Rice
Authors: Mohanty Shakti Prakash , Nayak Deepak Kumar , Sanghamitra Priyadarsini , Barik Saumya Ranjan , Pandit Elssa , Behera Abhisarika , Pani Dipti Ranjan , Mohapatra Shibani , Raj K. R. Reshmi , Pradhan Kartik Chandra , Sahoo Chita Ranjan , Mohanty Mihir Ranjan , Behera Chinmayee , Panda Alok Kumar , Jena Binod Kumar , Behera Lambodar , Dash Prasanta K. , Pradhan Sharat Kumar
Published: April 2023

Seed vigor is the key performance parameter of good quality seed. A panel was prepared by shortlisting genotypes from all the phenotypic groups representing seedling growth parameters from a total of 278 germplasm lines. A wide variation was observed for the traits in the population. The panel was classified into four genetic structure groups. Fixation indices indicated the existence of linkage disequilibrium in the population. A moderate to high level of diversity parameters was assessed using 143 SSR markers. Principal component, coordinate, neighbor-joining tree and cluster analyses showed subpopulations with a fair degree of correspondence with the growth parameters. Marker–trait association analysis detected eight novel QTLs, namely qAGR4.1, qAGR6.1, qAGR6.2 and qAGR8.1 for absolute growth rate (AGR); qRSG6.1, qRSG7.1 and qRSG8.1 for relative shoot growth (RSG); and qRGR11.1 for relative growth rate (RGR), as analyzed by GLM and MLM. The reported QTL for germination rate (GR), qGR4-1, was validated in this population. Additionally, QTLs present on chromosome 6 controlling RSG and AGR at 221 cM and RSG and AGR on chromosome 8 at 27 cM were detected as genetic hotspots for the parameters. The QTLs identified in the study will be useful for improvement of the seed vigor trait in rice.