bluuebunny/crossref_metadata_2025 huggingface dataset:
Gentamicin Targets Acid Sphingomyelinase in Cancer: The Case of the Human Gastric Cancer NCI-N87 Cells
Authors: Albi Elisabetta , Cataldi Samuela , Ceccarini Maria Rachele , Conte Carmela , Ferri Ivana , Fettucciari Katia , Patria Federica Filomena , Beccari Tommaso , Codini Michela
Published: September 2019

Emerging literature implicates acid sphingomyelinase in tumor sensitivity/resistance to anticancer treatments. Gentamicin is a drug commonly used as an antimicrobial but its serendipity effects have been shown. Even though many evidences on the role of gentamicin in cancer have been reported, its mechanism of action is poorly understood. Here, we explored acid sphingomyelinase as a possible new target of gentamicin in cancer. Since gastric cancer is one of the most common cancers and represents the second cause of death in the world, we performed the study in NCI-N87 gastric cancer cell line. The effect of the drug resulted in the inhibition of cell proliferation, including a reduction of cell number and viability, in the decrease of MIB-1 proliferative index as well as in the upregulation of cyclin-dependent kinase inhibitor 1A and 1B (CDKN1A and CDKN1B), and growth arrest and DNA-damage 45A (GADD45A) genes. The cytotoxicity was apoptotic as shown by FACS analysis. Additionally, gentamicin reduced HER2 protein, indicating a minor tumor aggressiveness. To further define the involvement of sphingomyelin metabolism in the response to the drug, gene and protein expression of acid and neutral sphingomeylinase was analyzed in comparison with phosphatase and tensin homolog deleted on chromosome 10 (PTEN) and vitamin D receptor (VDR), molecules involved in cancer. Gentamicin induced a downregulation of PTEN, VDR, and neutral sphingomyelinase and a strong upregulation of acid sphingomyelinase. Of note, we identified the same upregulation of acid sphingomyelinase upon gentamicin treatment in other cancer cells and not in normal cells. These findings provide new insights into acid sphingomyelinase as therapeutic target, reinforcing studies on the potential role of gentamicin in anticancer therapy.
An Efficient Dummy-Based Location Privacy-Preserving Scheme for Internet of Things Services
Authors: Du Yongwen , Cai Gang , Zhang Xuejun , Liu Ting , Jiang Jinghua
Published: September 2019

With the rapid development of GPS-equipped smart mobile devices and mobile computing, location-based services (LBS) are increasing in popularity in the Internet of Things (IoT). Although LBS provide enormous benefits to users, they inevitably introduce some significant privacy concerns. To protect user privacy, a variety of location privacy-preserving schemes have been recently proposed. Among these schemes, the dummy-based location privacy-preserving (DLP) scheme is a widely used approach to achieve location privacy for mobile users. However, the computation cost of the existing dummy-based location privacy-preserving schemes is too high to meet the practical requirements of resource-constrained IoT devices. Moreover, the DLP scheme is inadequate to resist against an adversary with side information. Thus, how to effectively select a dummy location is still a challenge. In this paper, we propose a novel lightweight dummy-based location privacy-preserving scheme, named the enhanced dummy-based location privacy-preserving(Enhanced-DLP) to address this challenge by considering both computational costs and side information. Specifically, the Enhanced-DLP adopts an improved greedy scheme to efficiently select dummy locations to form a k-anonymous set. A thorough security analysis demonstrated that our proposed Enhanced-DLP can protect user privacy against attacks. We performed a series of experiments to verify the effectiveness of our Enhanced-DLP. Compared with the existing scheme, the Enhanced-DLP can obtain lower computational costs for the selection of a dummy location and it can resist side information attacks. The experimental results illustrate that the Enhanced-DLP scheme can effectively be applied to protect the user’s location privacy in IoT applications and services.
Editorial for the Special Issue on “Natural Language Processing and Text Mining”
Authors: Gamallo Pablo , Garcia Marcos
Published: September 2019

Natural language processing (NLP) and Text Mining (TM) are a set of overlapping strategies working on unstructured text [...]
Role of Nutrition and Exercise Programs in Reducing Blood Pressure: A Systematic Review
Authors: Jurik Roman , Stastny Petr
Published: September 2019

The combined effect of diet and strength training (ST) on blood pressure (BP) seems to be very important for the treatment of prehypertension and hypertension (HT). Therefore, the aim of this study was to determine whether ST alone or combined with nutrition or supplementation has an impact on the arterial pressure reduction in normotensive and hypertensive populations. A systematic computerized literature search was performed according to the PRISMA guidelines using PubMed, Scopus and Google Scholar; only English language studies published from 1999 until 2018 were included. This systematic search identified the results of 303 individuals from nine studies. The ST program alone had a similar effectiveness as the nutrition program (NP) alone; however, their combination did not result in increased effectiveness in terms of a high BP reduction. The consumption of L-citrulline had a similar effect as ST on lowering BP; on the other hand, caffeine led to an increase in BP during the ST session. Our data suggest that a combination of ST 2–3 times a week at moderate intensity and a NP seems to be equally effective in terms of lowering BP (systolic and diastolic) as ST and NP alone.
Evaluation of Slag Reaction Efficiency in Slag-Cement Mortars under Different Curing Temperature
Authors: Wang Liang , Quan Hongzhu , Li Qiuyi
Published: September 2019

At present, not many studies have considered methods to quantitatively evaluate the reaction efficiency of granulated blast furnace slag (GBFS) at different curing temperatures. For high volume slag concrete, when the replacement ratio exceeds a certain ’threshold’ value, the superfluous and ineffective slag will no longer react in concrete but simply behave as a fine aggregate, which may cause the decrement of strength. The ’threshold’ value depends on the reaction efficiency of slag. In this study, experiments on mortars with different replacement ratios by slag were conducted at different curing temperatures (20, 30, and 50 °C, respectively), the threshold values of effective replacement ratio by slag were comprehensively analyzed through the reaction efficiency of slag mortar. The results showed that the turning point of the strength curve with replacement ratio can be considered as the threshold value of the effective replacement ratio by slag in mortar. Along with the curing temperature enhancement, the threshold value of the effective replacement ratio by slag in concrete decreased, whereas the reaction efficiency of slag increased. Meanwhile, the analysis of cement effective coefficient (k value) and basicity was also calculated. Based on the obtained threshold values of effective replacement ratio at different curing temperatures, the formula for the determination of reaction efficiency coefficient of slag in the mortar can be established. Therefore, the reaction efficiency coefficient and upper limit of the effective replacement ratio of slag at different temperatures can be calculated more intuitively and quantitatively, providing a theoretical basis and reference for practical engineering applications.
Independent Domination Stable Trees and Unicyclic Graphs
Authors: Wu Pu , Jiang Huiqin , Nazari-Moghaddam Sakineh , Sheikholeslami Seyed Mahmoud , Shao Zehui , Volkmann Lutz
Published: September 2019

A set S ⊆ V ( G ) in a graph G is a dominating set if S dominates all vertices in G, where we say a vertex dominates each vertex in its closed neighbourhood. A set is independent if it is pairwise non-adjacent. The minimum cardinality of an independent dominating set on a graph G is called the independent domination number i ( G ) . A graph G is ID-stable if the independent domination number of G is not changed when any vertex is removed. In this paper, we study basic properties of ID-stable graphs and we characterize all ID-stable trees and unicyclic graphs. In addition, we establish bounds on the order of ID-stable trees.
Effects of Acute Exercise and Learning Strategy Implementation on Memory Function
Authors: Loprinzi Paul , Harris Faith , McRaney Kyle , Chism Morgan , Deming Raymond , Jones Timothy , Zou Liye , Tan Miaoqing
Published: September 2019

Background and Objectives: Long-term potentiation (LTP), the functional connectivity among neurons, is considered a mechanism of episodic memory. Both acute exercise and learning are thought to influence memory via an LTP-related mechanism. Limited research has evaluated the individual and combined effects of acute exercise and learning strategy implementation (e.g., 3-R technique, cue-integration) on memory, which was the purpose of this study. Materials and Methods: For Experiment 1, participants (n = 80; Mage = 20.9 years) were randomized into one of four experimental groups, including Exercise + Learning (E + L), Learning Only (L), Exercise Only (E), and Control Group (C; no exercise and no learning strategy implementation). The exercise stimulus involved an acute 15-min bout of lower-intensity (60% of heart rate max) walking exercise and the learning strategy involved the implementation of the 3-R technique. Experiment 2 (n = 77; Mage = 21.1 years) replicated Experiment 1 but addressed limitations (e.g., exposure level of the memory task) from Experiment 1 and employed a higher-intensity bout of exercise (77% of heart rate max). Experiment 3 (n = 80; Mage = 21.0 years) evaluated these same four experimental conditions but employed a cue-integration learning strategy and a moderate-intensity bout of acute exercise (64% of heart rate max). Results: These three experiments demonstrate that both learning techniques were effective in enhancing memory and we also provided evidence of a main effect for acute exercise (Experiment 3). However, we did not observe consistent evidence of a learning by exercise interaction effect. Conclusions: We demonstrate that both acute exercise and different learning techniques are effective in enhancing long-term memory function.
Comparison of Hot Cracking Susceptibility of TIG and Laser Beam Welded Alloy 718 by Varestraint Testing
Authors: Alvarez Pedro , Vázquez Lexuri , Ruiz Noelia , Rodríguez Pedro , Magaña Ana , Niklas Andrea , Santos Fernando
Published: September 2019

Reduced hot cracking susceptibility is essential to ensure the flawless manufacturing of nickel superalloys typically employed in welded aircraft engine structures. The hot cracking of precipitation strengthened alloy 718 mainly depends on chemical composition and microstructure resulting from the thermal story. Alloy 718 is usually welded in a solution annealed state. However, even with this thermal treatment, cracks can be induced during standard industrial manufacturing conditions, leading to costly and time-consuming reworking. In this work, the cracking susceptibility of wrought and investment casting alloy 718 is studied by the Varestraint test. The test is performed while applying different welding conditions, i.e., continuous tungsten inert gas (TIG), low frequency pulsed TIG, continuous laser beam welding (LBW) and pulsed LBW. Welding parameters are selected for each welding technology in order to meet the welding quality criteria requested for targeted aeronautical applications, that is, full penetration, minimum cross-sectional welding width and reduced overhang and underfill. Results show that the hot cracking susceptibility of LBW samples determined by the Varestraint test is enhanced due to extended center line hot cracking, resulting in a fish-bone like cracking pattern. On the contrary, the minor effect of material source (wrought or casting), grain size and pulsation is observed. In fact, casting samples with a 30 times coarser grain size have shown better performance than wrought material.
Green Preparation and Dry Sliding Wear Properties of a Macro-ZTA/Fe Composite Produced by a Two-Step Method
Authors: Qiu Bo , Xing Shuming , Dong Qi
Published: September 2019

In this paper, a two-step method, rapid-flow mixing followed by high-pressure compositing was used to prepare a macro-ZTA (ZrO2-toughened Al2O3) particles reinforced high chromium cast iron (HCCI) matrix composite. The method is based on the squeeze casting process without general casting pollution problems. The microstructure, mechanical properties and dry sliding wear performance of the fabricated composite were investigated. The results showed that the particles were distributed uniformly throughout the iron matrix and a tightly bonded interface was obtained. Under dry sliding wear conditions, the wear resistance of the composite was significantly improved in comparison with the HCCI alloy, and the relative wear resistance was 1.8 and 2.9 times at the applied load of 300 and 900 N, respectively. When the load increased from 300 N to 900 N, the wear characteristics of the composite changed from shallow and narrow grooves and scratches to damages in the form of fragmentation of particles, transfer layer and interface cracking.
Antibacterial Activity of Three Extra Virgin Olive Oils of the Campania Region, Southern Italy, Related to Their Polyphenol Content and Composition
Authors: Nazzaro Filomena , Fratianni Florinda , Cozzolino Rosaria , Martignetti Antonella , Malorni Livia , De Feo Vincenzo , Cruz Adriano G. , d’Acierno Antonio
Published: September 2019

Production of extra virgin olive oil (EVOO) represents an important element for the economy of Southern Italy. Therefore, EVOO is recognized as a food with noticeable biological effects. Our study aimed to evaluate the antimicrobial activity exhibited by the polyphenolic extracts of EVOOs, obtained from three varieties of Olea europea L. (Ruvea antica, Ravece, and Ogliarola) cultivated in the village of Montella, Avellino, Southern Italy. The study evaluated the inhibiting effect of the extracts against some Gram-positive and Gram-negative bacteria. Statistical analysis, used to relate values of antimicrobial activity to total polyphenols and phenolic composition, revealed a different behavior among the three EVOO polyphenol extracts. The method applied could be useful to predict the influence of singular metabolites on the antimicrobial activity.
Organs, Cultivars, Soil, and Fruit Properties Affect Structure of Endophytic Mycobiota of Pinggu Peach Trees
Authors: Ren Fei , Dong Wei , Yan Dong-Hui
Published: September 2019

Pinggu peach (Prunus persica (L.)) has great economic and ecological value in north China. As a plant, the peach is naturally colonized by a variety of endophytic fungi, which are very important for tree growth and health. However, the mycobiota composition and their affecting factors of the peach trees are still unknown. In our study, the fungal communities in flowers, leaves, stems, and roots of the three cultivars (Dajiubao, Qingfeng, and Jingyan) of Pinggu peach trees and in the rhizosphere soils were investigated by both Illumina Miseq sequencing of ITS rDNA and traditional culturing methods. For organs, except for roots, flowers had the highest fungal richness and diversity, while the leaves had the lowest richness and diversity. Ascomycota and Basidiomycota were the most abundant phyla among samples. The fungal assemblage composition of each organ was distinctive. Fungal communities of the three cultivars also differed from each other. The fungal community structure significantly correlated with soil pH, soil K, fruit soluble solid content, and fruit titratable acidity with the redundancy analysis (RDA). Most isolated fungal strains can be found within high-throughput sequencing identified taxa. This study indicates that plant organs, the cultivars, the soil, and fruit properties may have profound effects on the endophytic fungal community structure associated with Pinggu peach trees. With this study, microbiota-mediated pathogen protection and fruit quality promotion associated with peach trees could be further studied.
SB365, Pulsatilla Saponin D Induces Caspase-Independent Cell Death and Augments the Anticancer Effect of Temozolomide in Glioblastoma Multiforme Cells
Authors: Hong Jun-Man , Kim Jin-Hee , Kim Hyemin , Lee Wang Jae , Hwang Young-il
Published: September 2019

SB365, a saponin D extracted from the roots of Pulsatilla koreana, has been reported to show cytotoxicity in several cancer cell lines. We investigated the effects of SB365 on U87-MG and T98G glioblastoma multiforme (GBM) cells, and its efficacy in combination with temozolomide for treating GBM. SB365 exerted a cytotoxic effect on GBM cells not by inducing apoptosis, as in other cancer cell lines, but by triggering caspase-independent cell death. Inhibition of autophagic flux and neutralization of the lysosomal pH occurred rapidly after application of SB365, followed by deterioration of mitochondrial membrane potential. A cathepsin B inhibitor and N-acetyl cysteine, an antioxidant, partially recovered cell death induced by SB365. SB365 in combination with temozolomide exerted an additive cytotoxic effect in vitro and in vivo. In conclusion, SB365 inhibits autophagic flux and induces caspase-independent cell death in GBM cells in a manner involving cathepsin B and mainly reactive oxygen species, and its use in combination with temozolomide shows promise for the treatment of GBM.
N…C and S…S Interactions in Complexes, Molecules, and Transition Structures HN(CH)SX:SCO, for X = F, Cl, NC, CCH, H, and CN
Authors: Del Bene Janet E. , Alkorta Ibon , Elguero José
Published: September 2019

Ab initio Møller–Plesset perturbation theory (MP2)/aug’-cc-pVTZ calculations have been carried out in search of complexes, molecules, and transition structures on HN(CH)SX:SCO potential energy surfaces for X = F, Cl, NC, CCH, H, and CN. Equilibrium complexes on these surfaces have C1 symmetry, but these have binding energies that are no more than 0.5 kJ·mol–1 greater than the corresponding Cs complexes which are vibrationally averaged equilibrium complexes. The binding energies of these span a narrow range and are independent of the N–C distance across the tetrel bond, but they exhibit a second-order dependence on the S–S distance across the chalcogen bond. Charge-transfer interactions stabilize all of these complexes. Only the potential energy surfaces HN(CH)SF:SCO and HN(CH)SCl:SCO have bound molecules that have short covalent N–C bonds and significantly shorter S…S chalcogen bonds compared to the complexes. Equation-of-motion coupled cluster singles and doubles (EOM-CCSD) spin-spin coupling constants 1tJ(N–C) for the HN(CH)SX:SCO complexes are small and exhibit no dependence on the N–C distance, while 1cJ(S–S) exhibit a second-order dependence on the S–S distance, increasing as the S–S distance decreases. Coupling constants 1tJ(N–C) and 1cJ(S–S) as a function of the N–C and S–S distances, respectively, in HN(CH)SF:SCO and HN(CH)SCl:SCO increase in the transition structures and then decrease in the molecules. These changes reflect the changing nature of the N…C and S…S bonds in these two systems.
Computational Study of Natural Compounds for the Clearance of Amyloid-Βeta: A Potential Therapeutic Management Strategy for Alzheimer’s Disease
Authors: Ahmad Syed Sayeed , Khan Haroon , Danish Rizvi Syed Mohd. , Ansari Siddique Akber , Ullah Riaz , Rastrelli Luca , Mahmood Hafiz Majid , Siddiqui Mohd. Haris
Published: September 2019

Alzheimer’s disease (AD) is a widespread dynamic neurodegenerative malady. Its etiology is still not clear. One of the foremost pathological features is the extracellular deposits of Amyloid-beta (Aβ) peptides in senile plaques. The interaction of Aβ and the receptor for advanced glycation end products at the blood-brain barrier is also observed in AD, which not only causes the neurovascular anxiety and articulation of proinflammatory cytokines, but also directs reduction of cerebral bloodstream by upgrading the emission of endothelin-1 to induce vasoconstriction. In this process, RAGE is deemed responsible for the influx of Aβ into the brain through BBB. In the current study, we predicted the interaction potential of the natural compounds vincamine, ajmalicine and emetine with the Aβ peptide concerned in the treatment of AD against the standard control, curcumin, to validate the Aβ peptide–compounds results. Protein-protein interaction studies have also been carried out to see their potential to inhibit the binding process of Aβ and RAGE. Moreover, the current study verifies that ligands are more capable inhibitors of a selected target compared to positive control with reference to ΔG values. The inhibition of Aβ and its interaction with RAGE may be valuable in proposing the next round of lead compounds for effective Alzheimer’s disease treatment.
Synthesis of Cyclobutane Analogue 4: Preparation of Purine and Pyrimidine Carbocyclic Nucleoside Derivatives
Authors: Hasaneen Noha , Ebead Abdelaziz , Hassan Muhammad Murtaza , Afifi Hanan , Hunter Howard , Lee-Ruff Edward , El-Gohary Nadia , Maarouf Azza , El-Emam Ali
Published: September 2019

The coupling of 2-bromo-3-benzoyloxycyclobutanone with purine under basic conditions produces two regioisomers consisting of the N-7 and N-9 alkylated products in equal amounts in their racemic forms. The distribution of the isomers is consistent with the charge delocalization between the N-7 and N-9 positions of the purinyl anion. The structural assignments and relative stereochemistry of each regioisomer were based on 1 and 2D NMR techniques. The relative stereochemistry of the C-2 and C-3 substituents in each regioisomer was the trans orientation consistent with steric factors in the coupling step. The N-9 regioisomer was reduced with sodium borohydride to give the all trans cyclobutanol as the major product in a stereoselective manner. The alcohol was debenzoylated with sodium methoxide in a transesterification step to give the nucleoside analogue. The regioisomeric pyrimidine nucleosides were prepared by Vorbrüggen coupling of the 3-hydroxymethylcyclobutanone triflate with either thymine or uracil followed by stereoselective hydride addition. Regiospecificity of the coupling at the N-1 position was observed and stereoselective reduction to the trans-disubstituted cyclobutanol structure assignments was based on NMR data.
Evaluation of Elastin-Like Polypeptides for Tumor Targeted Delivery of Doxorubicin to Glioblastoma
Authors: Dragojevic Sonja , Mackey Rebecca , Raucher Drazen
Published: September 2019

To increase treatment efficiency for glioblastoma, we have developed a system to selectively deliver chemotherapeutic doxorubicin (Dox) to Glioblastoma (GBM) tumors. This carrier is based on elastin-like polypeptide (ELP), which is soluble at physiological temperatures but undergoes a phase transition and accumulates at tumor sites with externally applied, mild (40–41 °C) hyperthermia. The CPP-ELP-Dox conjugate consists of a cell penetrating peptide (CPP), which facilitates transcytosis through the blood brain barrier and cell entry, and a 6-maleimidocaproyl hydrazone derivative of doxorubicin at the C-terminus of ELP. The acid-sensitive hydrazone linker ensures release of Dox in the lysosomes/endosomes after cellular uptake of the drug conjugate. We have shown that CPP-ELP-Dox effectively inhibits cell proliferation in three GBM cell lines. Both the free drug and CPP-ELP-Dox conjugate exhibited similar in vitro cytotoxicity, although their subcellular localization was considerably different. The Dox conjugate was mainly dispersed in the cytoplasm, while free drug had partial nuclear accumulation in addition to cytoplasmic distribution. The intracellular Dox concentration was increased in the CPP-ELP-Dox cells compared to that in the cells treated with free Dox, which positively correlates with cytotoxic activity. In summary, our findings demonstrate that CPP-ELP-Dox effectively kills GBM cells. Development of such a drug carrier has the potential to greatly improve current therapeutic approaches for GBM by increasing the specificity and efficacy of treatment and reducing cytotoxicity in normal tissues.
Reviewing HIV-1 Gag Mutations in Protease Inhibitors Resistance: Insights for Possible Novel Gag Inhibitor Designs
Authors: Su Chinh Tran-To , Koh Darius Wen-Shuo , Gan Samuel Ken-En
Published: September 2019

HIV protease inhibitors against the viral protease are often hampered by drug resistance mutations in protease and in the viral substrate Gag. To overcome this drug resistance and inhibit viral maturation, targeting Gag alongside protease rather than targeting protease alone may be more efficient. In order to successfully inhibit Gag, understanding of its drug resistance mutations and the elicited structural changes on protease binding needs to be investigated. While mutations on Gag have already been mapped to protease inhibitor resistance, there remain many mutations, particularly the non-cleavage mutations, that are not characterized. Through structural studies to unravel how Gag mutations contributes to protease drug resistance synergistically, it is thus possible to glean insights to design novel Gag inhibitors. In this review, we discuss the structural role of both novel and previously reported Gag mutations in PI resistance, and how new Gag inhibitors can be designed.
A Secreted Phospholipase A2 Induces Formation of Smooth Muscle Foam Cells Which Transdifferentiate to Macrophage-Like State
Authors: Giannotti Karina Cristina , Weinert Sönke , Viana Mariana Nascimento , Leiguez Elbio , Araujo Thaís L. S. , Laurindo Francisco R. M. , Lomonte Bruno , Braun-Dullaeus Rüdiger , Teixeira Catarina
Published: September 2019

Vascular smooth muscle cells (VSMCs) loaded with lipid droplets (LDs) are markers of atherosclerosis. In this disease, inflammatory Group IIA-secreted phospholipase A2s (GIIA sPLA2s) are highly expressed in VSMCs, but their actions in these cells are unknown. Here, we investigated the ability of myotoxin III (MT-III), an ophidian GIIA sPLA2 sharing structural and functional features with mammalian GIIA sPLA2s, to induce LD formation and lipid metabolism factors involved in this effect. Modulation of VSMC phenotypes by this sPLA2 was also evaluated. Incubation of VSMCs with MT-III significantly increased the number of LDs. MT-III upregulated scavenger receptor type 1 (SR-A1) and lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) protein expression and enhanced acetylated-low density lipoprotein (acLDL) uptake by VSMCs, revealing the ability of a GIIA PLA2 to modulate scavenger receptor activities. MT-III induced translocation and protein expression of PPAR-γ and -β/δ. Inhibition of peroxisome proliferator-activated receptors (PPARs) and diacylglycerol O-acyltransferase (DGAT) and acyl-CoA:cholesterolacyltransferase (ACAT) enzymes abrogated MT-III-induced LD formation. Moreover, in response to MT-III, VSMCs acquired phagocytic activity and expressed macrophage markers CD68 and MAC-2. In conclusion, MT-III is able to stimulate VSMCs and recruit factors involved in lipid uptake and metabolism, leading to the formation of VSMC-derived foam cells with acquisition of macrophage-like markers and functions.
Short-Time Hydrothermal Synthesis of CuBi2O4 Nanocolumn Arrays for Efficient Visible-Light Photocatalysis
Authors: Wang Yi , Cai Fang , Guo Pengran , Lei Yongqian , Xi Qiaoyue , Wang Fuxian
Published: September 2019

In this article, a short-time hydrothermal method is developed to prepare CuBi2O4 nanocolumn arrays. By using Bi(NO3)3·5H2O in acetic acid and Cu(NO3)2·3H2O in ethanol as precursor solutions, tetragonal CuBi2O4 with good visible light absorption can be fabricated within 0.5 h at 120 °C. Tetragonal structured CuBi2O4 can be formed after 15 min hydrothermal treatment, however it possesses poor visible light absorption and low photocatalytic activity. Extending the hydrothermal treatment duration to 0.5 h results in a significant improvement invisible light absorption of the tetragonal CuBi2O4. The CuBi2O4 obtained through 0.5 h hydrothermal synthesis shows a band gap of 1.75 eV and exhibits the highest photocatalytic performance among the CuBi2O4 prepared with various hydrothermal time. The removal rate of methylene blue by the 0.5 h CuBi2O4 reaches 91% under visible light irradiation for 0.5 h. This study proposes a novel strategy to prepare photoactive CuBi2O4 nanocolumn arrays within 0.5 h at a moderate temperature of 120 °C. The hydrothermal method provides a facile strategy for the fast synthesis of metal-oxide-based photocatalysts at mild reaction conditions.
Morphological Transformation of Silver Nanoparticles from Commercial Products: Modeling from Product Incorporation, Weathering through Use Scenarios, and Leaching into Wastewater
Authors: Mohan Selvan , Princz Juliska , Ormeci Banu , DeRosa Maria C.
Published: September 2019

There is increasing interest in the environmental fate and effects of engineered nanomaterials due to their ubiquitous use in consumer products. In particular, given the mounting evidence that dramatic transformations can occur to a nanomaterial throughout its product lifecycle, the appropriateness of using pristine nanomaterials in environmental testing is being questioned. Using a combination of transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma-mass spectrometry (ICP-MS), this work examines the morphological and compositional effects of conditions mimicking a typical lifecycle of a nano-enabled product, from the production of the silver nanoparticle (AgNP)-laden textiles, through its use, laundering, and then finally, its leaching and incubation in the wastewater collection system. These simulated weathering conditions showed evidence for the transformation of AgNPs into AgCl and Ag2S. Incubation in raw wastewater had the most dramatic effect on the AgNPs in terms of transformation, no matter what initial weathering was applied to the NPs prior to incubation. However, despite extensive transformation noted, AgNPs were still present within all the samples after the use scenarios.
Enhancing the Photovoltaic Performance of Perovskite Solar Cells Using Plasmonic Au@Pt@Au Core-Shell Nanoparticles
Authors: Wang Bao , Zhu Xiangyu , Li Shuhan , Chen Mengwei , Liu Nan , Yang Hao , Ran Meiqing , Lu Haifei , Yang Yingping
Published: September 2019

Au@Pt@Au core-shell nanoparticles, synthesized through chemical reduction, are utilized to improve the photoelectric performance of perovskite solar cells (PSCs) in which carbon films are used as the counter electrode, and the hole-transporting layer is not used. After a series of experiments, these Au@Pt@Au core-shell nanoparticles are optimized and demonstrate outstanding optical and electrical properties due to their local surface plasmon resonance and scattering effects. PSC devices containing 1 wt.% Au@Pt@Au core-shell nanoparticles have the highest efficiency; this is attributable to their significant light trapping and utilization capabilities, which are the result of the distinctive structure of the nanoparticles. The power conversion efficiency of PSCs, with an optimal content of plasmonic nanoparticles (1 wt.%), increased 8.1%, compared to normal PSCs, which was from 12.4% to 13.4%; their short-circuit current density also increased by 5.4%, from 20.5 mA·cm−2 to 21.6 mA·cm−2. The open-circuit voltages remaining are essentially unchanged. When the number of Au@Pt@Au core-shell nanoparticles in the mesoporous TiO2 layer increases, the photovoltaic parameters of the former shows a downward trend due to the recombination of electrons and holes, as well as the decrease in electron transporting pathways.
Influence of Individual Ions on Silica Nanoparticles Interaction with Berea Sandstone Minerals
Authors: Hamouda Aly A. , Abhishek Rockey
Published: September 2019

Nanofluids are prepared by dispersing silica nanoparticles in aqueous media (brines). The purpose of this work is to address brine/rock interactions in presence of nanoparticles. Our previous studies have shown that silica nanofluids are effective in reducing formation damage in sandstone reservoirs. This study addresses effect of individual ions on dispersed silica nanoparticles’ interaction with Berea Sandstone minerals. The selected ions are Mg2+, SO42− and Na+, in MgCl2, Na2SO4 and NaCl, which are the major constituents of seawater. Three flooding stages for Berea Sandstone cores were followed. The first flooding stage was without nanoparticles, the second one was a slug of the nanoparticles with tracer and the third stage was a post-flushing of the core with the respective ion. The effluent tracer concentration, nanoparticle content, ion concentrations and pH reflect the effect of individual ions on nanoparticle/mineral interaction which were used for suggesting possible interaction mechanisms. Presence of Mg2+ and SO42− ions improved the adsorption of nanoparticles on minerals, however the effect of Na+ was lesser. In general, in all the cases nanoparticles reduced the mineral dissolution and associated fine migration/possible formation damage.
The Paradoxical Role of Uric Acid in Osteoporosis
Authors: Lin Kun-Mo , Lu Chien-Lin , Hung Kuo-Chin , Wu Pei-Chen , Pan Chi-Feng , Wu Chih-Jen , Syu Ren-Si , Chen Jin-Shuen , Hsiao Po-Jen , Lu Kuo-Cheng
Published: September 2019

Because of its high prevalence worldwide, osteoporosis is considered a serious public health concern. Many known risk factors for developing osteoporosis have been identified and are crucial if planning health care needs. Recently, an association between uric acid (UA) and bone fractures had been explored. Extracellular UA exhibits antioxidant properties by effectively scavenging free radicals in human plasma, but this benefit might be disturbed by the hydrophobic lipid layer of the cell membrane. In contrast, intracellular free oxygen radicals are produced during UA degradation, and superoxide is further enhanced by interacting with NADPH oxidase. This intracellular oxidative stress, together with inflammatory cytokines induced by UA, stimulates osteoclast bone resorption and inhibits osteoblast bone formation. UA also inhibits vitamin D production and thereby results in hyper-parathyroidism, which causes less UA excretion in the intestines and renal proximal tubules by inhibiting the urate transporter ATP-binding cassette subfamily G member 2 (ABCG2). At normal or high levels, UA is associated with a reduction in bone mineral density and protects against bone fracture. However, in hyperuricemia or gout arthritis, UA increases bone fracture risk because oxidative stress and inflammatory cytokines can increase bone resorption and decrease bone formation. Vitamin D deficiency, and consequent secondary hyperparathyroidism, can further increase bone resorption and aggravated bone loss in UA-induced osteoporosis.
Lycopene Inhibits Activation of Epidermal Growth Factor Receptor and Expression of Cyclooxygenase-2 in Gastric Cancer Cells
Authors: Han Hwana , Lim Joo Weon , Kim Hyeyoung
Published: September 2019

Reactive oxygen species (ROS) contribute to the oncogenic phenotype of cancer cells by acting as signaling molecules for inducing proliferation. ROS are known to activate the epidermal growth factor receptor (EGFR), which causes the activation of the Ras/mitogen-activated protein kinases (MAPKs) pathway. The Ras-dependent pathway promotes the activation of nuclear factor-kappa-light-chain-enhancer of activated B cells (NF-κB), a transcriptional modulator of cyclooxygenase-2 (COX-2) that induces cell proliferation. Lycopene is a potent antioxidant carotenoid and is responsible for the red color of fruits and vegetables. This study aims to investigate whether lycopene inhibits proliferation and induces apoptosis in gastric cancer AGS cells by suppressing the EGFR/Ras/MAPK and NF-κB-COX-2 signaling axis. Lycopene decreased cell viability and increased apoptotic indices (DNA fragmentation, apoptosis inducing factor, cleavage of caspase-3 and caspase-9, Bax/Bcl-2 ratio). Lycopene reduced the level of intracellular and mitochondrial ROS and decreased the activation of the ROS-mediated EGFR/Ras/extracellular signal-regulated kinase (ERK) and p38 MAPK pathways, thus leading to attenuation of the DNA-binding activity of NF-κB p50/p50 and the level of COX-2 gene expression. These results show that lycopene-induced apoptosis and inhibition of proliferation occur via inhibition of ROS-activated EGFR/Ras/ERK and p38 MAPK pathways and NF-κB-mediated COX-2 gene expression in AGS cells. In conclusion, consumption of lycopene-enriched foods could decrease the incidence of gastric cancer.
In Utero Dexamethasone Exposure Exacerbates Hepatic Steatosis in Rats That Consume Fructose During Adulthood
Authors: Payolla Tanyara B. , Teixeira Caio J. , Sato Fabio T. , Murata Gilson M. , Zonta Gizela A. , Sodré Frhancielly S. , Campos Carolina V. , Mesquita Filiphe N. , Anhê Gabriel F. , Bordin Silvana
Published: September 2019

Distinct environmental insults might interact with fructose consumption and contribute to the development of metabolic disorders. To address whether in utero glucocorticoid exposure and fructose intake modulate metabolic responses, adult female Wistar rats were exposed to dexamethasone (DEX) during pregnancy, and the offspring were administered fructose at a later time. Briefly, dams received DEX during the third period of pregnancy, while control dams remained untreated. Offspring born to control and DEX-treated mothers were defined as CTL-off and DEX-off, respectively, while untreated animals were designated CTL-off-CTL and DEX-off-CTL. CLT-off and DEX-off treated with 10% fructose in the drinking water for 8 weeks are referred to as CTL-off-FRU and DEX-off-FRU. We found that fructose promoted glucose intolerance and whole-body gluconeogenesis in both CTL-off-FRU and DEX-off-FRU animals. On the other hand, hepatic lipid accumulation was significantly stimulated in DEX-off-FRU rats when compared to the CTL-off-FRU group. The DEX-off-FRU group also displayed impaired very-low-density lipoprotein (VLDL) production and reduced hepatic expression of apoB, mttp, and sec22b. DEX-off-FRU has lower hepatic levels of autophagy markers. Taken together, our results support the unprecedented notion that in utero glucocorticoid exposure exacerbates hepatic steatosis caused by fructose consumption later in life.
Retention of Primary Bile Acids by Lupin Cell Wall Polysaccharides Under In Vitro Digestion Conditions
Authors: Naumann Susanne , Schweiggert-Weisz Ute , Haller Dirk , Eisner Peter
Published: September 2019

Interference of dietary fibres with the enterohepatic circulation of bile acids is proposed as a mechanism for lowering cholesterol. We investigated how lupin hull and cotyledon dietary fibres interact with primary bile acids using an in vitro model under simulated upper gastrointestinal conditions. Cell wall polysaccharides were isolated and extracted to separate pectin-like, hemicellulosic, and lignocellulosic structures. Lupin hull consisted mainly of structural components rich in cellulose. The viscosity of the in vitro digesta of lupin hull was low, showing predominantly liquid-like viscoelastic properties. On the other hand, lupin cotyledon fibre retarded bile acid release due to increased viscosity of the in vitro digesta, which was linked with high contents of pectic polymers forming an entangled network. Molecular interactions with bile acids were not measured for the hull but for the cotyledon, as follows: A total of 1.29 µmol/100 mg DM of chenodesoxycholic acids were adsorbed. Molecular interactions of cholic and chenodesoxycholic acids were evident for lignin reference material but did not account for the adsorption of the lupin cotyledon. Furthermore, none of the isolated and fractionated cell wall materials showed a significant adsorptive capacity, thus disproving a major role of lupin cell wall polysaccharides in bile acid adsorption.
Alcohol Consumption and Risk of Chronic Kidney Disease: A Nationwide Observational Cohort Study
Authors: Lai Yun-Ju , Chen Yu-Yen , Lin Yu-Kai , Chen Chu-Chieh , Yen Yung-Feng , Deng Chung-Yeh
Published: September 2019

Alcohol consumption is a significant public health issue worldwide. The rat model and epidemiological studies have both reported conflicting results about the effects of alcohol on the kidneys. We aimed to explore the relationships between alcohol consumption and chronic kidney disease. Data from the National Health Interview Survey, the National Health Insurance research database, and the National Deaths Dataset were used. Standardized in-person interviews were executed in 2001, 2005, and 2009 to obtain the demographic characteristics of study population. The participants were followed up until 2013. The primary outcome was new-onset chronic kidney disease. We analyzed 45,200 adults older than 18 years (50.8% men and 49.2% women), and the overall mean (SD) age was 42.73 (16.64) years. During the 8.5 (3.5) years of follow-up, new-onset chronic kidney disease was recognized in 1535 (5.5%), 292 (2.7%), and 317 (4.9%) non-drinking, social-drinking, and regular-drinking participants, respectively. The participants who were social and regular drinkers had a significantly decreased risk of chronic kidney disease incidence (social drinking: adjusted hazard ratio (HR), 0.85; 95% confidence interval (CI), 0.74–0.97; p = 0.018; regular-drinking: AHR, 0.85; 95% CI, 0.74–0.98; p = 0.024), with baseline demographics and comorbidities adjusted. In conclusion, social and regular drinkers had decreased risk of chronic kidney disease when compared with non-drinkers.
Energy Balance and Active Lifestyle: Potential Mediators of Health and Quality of Life Perception in Aging
Authors: Condello Giancarlo , Capranica Laura , Migliaccio Silvia , Forte Roberta , Di Baldassarre Angela , Pesce Caterina
Published: September 2019

The relationship between aging and perception of health and quality of life is complex and its mediation mechanisms need to be further explored. The purpose of this study was to investigate the mediating effects of total energy expenditure and intake, body mass, and body image dissatisfaction on the relationship between age and perception of health and quality of life. Forty-two senior athletes, 55 physically active, and 61 sedentary individuals (aged 55–84 years) were evaluated for total energy expenditure (EE), energy intake (EI), body mass index (BMI), absolute Body Dissatisfaction Index (BDIabx), and physical (PCS) and mental (MCS) health and quality of life perception. Multiple mediation analyses were applied to assess the relationship between age and PCS and MCS indices, through the mediators EE, EI, BMI, and BDIabx. For MCS, but not for PSC, the mediation analysis showed: (a) a direct effect of age; (b) a mediation path through EE, EI, BMI, and BDIabx; and (c) a positive total effect. The combination of positive and negative mediating effects influencing the mental health perception underlined that with advancing age, the maintenance of high levels of energy expenditure through physical activity could positively impact body image satisfaction and, in turn, mental health.
Discrimination of Myrtle Ecotypes from Different Geographic Areas According to Their Morphological Characteristics and Anthocyanins Composition
Authors: González-de-Peredo Ana V. , Vázquez-Espinosa Mercedes , Espada-Bellido Estrella , Ferreiro-González Marta , Amores-Arrocha Antonio , Palma Miguel , Barbero Gerardo F. , Jiménez-Cantizano Ana
Published: September 2019

Myrtus communis L. is an evergreen shrub that produces berries with a high content in antioxidant compounds. Since these compounds have demonstrated a positive effect on human health, the interest on berries and their usages has increased. However, environmental conditions may affect the productivity of these species and consequently the quality of wild myrtle. Ecotypes from diverse geographical origins may result in significant variations in terms of bioactive compounds content as well as in chemical traits. For this reason, in this work ecotypes from two different localizations have been studied to determine if their differences in morphological and anthocyanins traits can be attributed to their origin and the environmental characteristics of these locations. For this, chemometric analyses such as Hierarchical Cluster Analysis and Principal Component Analysis, were employed. The results showed differences between the ecotypes depending on their location. In particular, myrtle berries from maritime zones present greater fruit size and amount of bioactive compounds, which means an improvement in the quality of the final product based on this raw material. It can be concluded that both morphological and anthocyanins traits are related to the location of the ecotype and allow selecting the best ecotype for the required applications.
The Performance and Exhaust Emissions of a Diesel Engine Fuelled with Calophyllum inophyllum—Palm Biodiesel
Authors: Damanik Natalina , Ong Hwai Chyuan , Mofijur M. , Tong Chong Wen , Silitonga Arridina Susan , Shamsuddin Abd Halim , Sebayang Abdi Hanra , Mahlia Teuku Meurah Indra , Wang Chin-Tsan , Jang Jer-Huan
Published: September 2019

Nowadays, increased interest among the scientific community to explore the Calophyllum inophyllum as alternative fuels for diesel engines is observed. This research is about using mixed Calophyllum inophyllum-palm oil biodiesel production and evaluation that biodiesel in a diesel engine. The Calophyllum inophyllum–palm oil methyl ester (CPME) is processed using the following procedure: (1) the crude Calophyllum inophyllum and palm oils are mixed at the same ratio of 50:50 volume %, (2) degumming, (3) acid-catalysed esterification, (4) purification, and (5) alkaline-catalysed transesterification. The results are indeed encouraging which satisfy the international standards, CPME shows the high heating value (37.9 MJ/kg) but lower kinematic viscosity (4.50 mm2/s) due to change the fatty acid methyl ester (FAME) composition compared to Calophyllum inophyllum methyl ester (CIME). The average results show that the blended fuels have higher Brake Specific Fuel Consumption (BSFC) and NOx emissions, lower Brake Thermal Efficiency (BTE), along with CO and HC emissions than diesel fuel over the entire range of speeds. Among the blends, CPME5 offered better performance compared to other fuels. It can be recommended that the CPME blend has great potential as an alternative fuel because of its excellent characteristics, better performance, and less harmful emission than CIME blends.
Insights into Kinetics of Methane Hydrate Formation in the Presence of Surfactants
Authors: Pandey Jyoti Shanker , Daas Yousef Jouljamal , Solms Nicolas von
Published: September 2019

Sodium dodecyl sulfate (SDS) is a well-known surfactant, which can accelerate methane hydrate formation. In this work, methane hydrate formation kinetics were studied in the presence of SDS using a rocking cell apparatus in both temperature-ramping and isothermal modes. Ramping and isothermal experiments together suggest that SDS concentration plays a vital role in the formation kinetics of methane hydrate, both in terms of induction time and of final gas uptake. There is a trade-off between growth rate and gas uptake for the optimum SDS concentration, such that an increase in SDS concentration decreases the induction time but also decreases the gas storage capacity for a given volume. The experiments also confirm the potential use of the rocking cell for investigating hydrate promoters. It allows multiple systems to run in parallel at similar experimental temperature and pressure conditions, thus shortening the total experimentation time. Understanding methane hydrate formation and storage using SDS can facilitate large-scale applications such as natural gas storage and transportation.