bluuebunny/crossref_metadata_2025 huggingface dataset:
An Explorative Evaluation on the Influence of Filler Content of Polyetheretherketone (PEEK) on Adhesive Bond to Different Luting Resin Cements
Authors: Dederichs Marco , Lackner Oliver , Kuepper Harald , Decker Mike , Viebranz Stephanie , Hennig Christoph-Ludwig , Guentsch Arndt , Kuepper Christine
Published: November 2023

Polyetheretherketone (PEEK) is considered one of the most innovative prosthetic materials of the last few decades. Its chemically inert behavior and high biocompatibility make it a promising material in many areas of dentistry. The aim of this study was to test whether PEEK with different TiO2 filler contents achieves comparable bond strength values when using different resin cements. N = 70 PEEK samples each with different TiO2 filler content (20 wt.% TiO2 vs. 5 wt.% TiO2 vs. no filler as a control group) were divided into seven groups and cemented with various conventional (ResiCem, RelyX Ultimate, Variolink Esthetic DC) and self-adhesive resin cements (RelyXUnicem 2, Bifix SE, Panavia SA Cement Plus, SpeedCem). The shear strength of the bond was assessed after 24 h and after 25,000 thermal loading cycles. Mann-Whitney U and Wilcoxon tests were used for statistical analysis (significance level: α = 0.05). PEEK without filler showed the highest mean shear strength (24.26 MPa using RelyX Ultimate), then high-filled PEEK (22.90 MPa using ResiCem) and low-filled PEEK (21.76 MPa using RelyX Ultimate). Conventional resin cements generally achieved slightly higher adhesive strengths than self-adhesive resin cements. It appears that the filler content does not affects the adhesive bond strengths.
Metacarpophalangeal Joint Reconstruction of a Complex Hand Injury with a Vascularized Lateral Femoral Condyle Flap Using an Individualized 3D Printed Model—A Case Report
Authors: Kohlhauser Michael , Vasilyeva Anna , Kamolz Lars-Peter , Bürger Heinz K. , Schintler Michael
Published: November 2023

This case report describes the surgical management of a patient with a complex hand trauma. This injury included tendon, vascular, and nerve injuries, a partial amputation of the index finger, fractures of the third proximal phalanx, and destruction of the metacarpophalangeal joint of the fifth finger. Firstly, the acute treatment of a complex hand injury is described. Secondly, the planning and execution of a joint reconstruction using a vascularized lateral femoral condylar flap, assisted by an individual 3D model, is illustrated. Precise reconstruction of the affected structures resulted in good revascularization as well as an anatomical bone consolidation. Intensive physical therapy, including autonomous proprioceptive range-of-motion exercises by the patient, resulted in significant functional improvement of the hand in daily life. Overall, we report on the successful reconstruction of a metacarpophalangeal joint by using a vascularized flap from the lateral femoral condyle. Furthermore, this case report highlights the efficacy of integrating individualized 3D printing technology to plan complex reconstructions, opening up promising opportunities for personalized and optimized interventions.
Study on the Ecosystem Service Supply–Demand Relationship and Development Strategies in Mountains in Southwest China Based on Different Spatial Scales
Authors: Wang Yahui , Dai Erfu , Qi Yue , Fan Yao
Published: November 2023

Mountainous regions typically exhibit a strained relationship between humans and the land, with noticeable spatial differences in the supply and demand of ecosystem services (ESS and ESD, respectively). ESS and ESD display varying characteristics at different spatial scales. Research on ESS and ESD at multiple scales can aid regional development and efficient ecosystem management. However, the current research focuses on ESS and neglects the ES characteristic changes at different scales. This study concentrates on the Hengduan Mountain region in southwest China. It evaluated ESS and ESD, analyzed the spatial matching relationship, and proposed a corresponding development strategy. The results demonstrated four key findings. First, ESS displayed an inverse spatial distribution on the two scales and was lower in the north and higher in the south at the raster scale. Over the period 2000–2020, ESS exhibited a pattern of initial increase, followed by a decrease, albeit with varying spatial patterns. Changes in land use primarily drove these ESS changes. Second, ESD increased from northwest to southeast on both scales and showed a rising trend over time. Third, at the grid scale, the low supply and low demand (L-L) region is primarily situated in the northwestern part, and it is crucial to prevent grassland degradation and manage grazing intensity. The low supply and high demand (L-H) region is located in the southeast, where the protection of cultivated land, along with comprehensive control of rocky desertification and debris flow, should be prioritized. High supply and low demand (H-L) are found in the northern mountain area, where paying attention to soil erosion control is essential. For areas with high supply and high demand (H-H) types, efforts should be directed toward maintaining forest habitat integrity. Fourth, on the county scale, L-H types should focus on realizing the ecosystem service value and implementing ecological agriculture. H-L counties can appropriately develop economic activities. Simultaneously, ecological compensation should be conducted among counties.
Effects of Fenofibrate and Gemfibrozil on Kynurenic Acid Production in Rat Kidneys In Vitro: Old Drugs, New Properties
Authors: Zakrocka Izabela , Kocki Tomasz , Urbańska Ewa , Załuska Wojciech
Published: November 2023

Kidney dysfunction significantly increases the cardiovascular risk, even in cases of minor functional declines. Hypertriglyceridemia is the most common lipid abnormality reported in patients with kidney disorders. PPAR-α (peroxisome proliferator-activated receptor-α) agonists called fibrates are the main agents used to lower triglyceride levels. Kynurenic acid (KYNA) is a tryptophan (Trp) derivative directly formed from L-kynurenine (L-KYN) by kynurenine aminotransferases (KATs). KYNA is classified as a uremic toxin, the level of which is correlated with kidney function impairments and lipid abnormalities. The aim of this study was to analyze the effect of the most commonly used triglyceride-lowering drugs, fenofibrate and gemfibrozil, on KYNA production and KAT activity in rat kidneys in vitro. The influence of fenofibrate and gemfibrozil on KYNA formation and KAT activity was tested in rat kidney homogenates in vitro. Fenofibrate and gemfibrozil at 100 µM–1 mM significantly inhibited KYNA synthesis in rat kidney homogenates. Both fibrates directly affected the KAT I and KAT II isoenzyme activities in a dose-dependent manner at similar concentrations. The presented results reveal the novel mechanism of action of fibrates in the kidneys and suggest their potential role in kidney function protection beyond the well-known anti-hyperlipidemic effect.
Therapeutic Potential of P110 Peptide: New Insights into Treatment of Alzheimer’s Disease
Authors: Srivastava Ankita , Johnson Maryann , Renna Heather A. , Sheehan Katie M. , Ahmed Saba , Palaia Thomas , Pinkhasov Aaron , Gomolin Irving H. , De Leon Joshua , Reiss Allison B.
Published: November 2023

Mitochondrial degeneration in various neurodegenerative diseases, specifically in Alzheimer’s disease, involves excessive mitochondrial fission and reduced fusion, leading to cell damage. P110 is a seven-amino acid peptide that restores mitochondrial dynamics by acting as an inhibitor of mitochondrial fission. However, the role of P110 as a neuroprotective agent in AD remains unclear. Therefore, we performed cell culture studies to evaluate the neuroprotective effect of P110 on amyloid-β accumulation and mitochondrial functioning. Human SH-SY5Y neuronal cells were incubated with 1 µM and 10 µM of P110, and Real-Time PCR and Western blot analysis were done to quantify the expression of genes pertaining to AD and neuronal health. Exposure of SH-SY5Y cells to P110 significantly increased APP mRNA levels at 1 µM, while BACE1 mRNA levels were increased at both 1 µM and 10 µM. However, protein levels of both APP and BACE1 were significantly reduced at 10 µM of P110. Further, P110 treatment significantly increased ADAM10 and Klotho protein levels at 10 µM. In addition, P110 exposure significantly increased active mitochondria and reduced ROS in live SH-SY5Y cells at both 1 µM and 10 µM concentrations. Taken together, our results indicate that P110 might be useful in attenuating amyloid-β generation and improving neuronal health by maintaining mitochondrial function in neurons.
Evolving Hybrid Deep Neural Network Models for End-to-End Inventory Ordering Decisions
Authors: de Castro Moraes Thais , Qin Jiancheng , Yuan Xue-Ming , Chew Ek Peng
Published: November 2023

Background: Over the past decade, the potential advantages of employing deep learning models and leveraging auxiliary data in data-driven end-to-end (E2E) frameworks to enhance inventory decision-making have gained recognition. However, current approaches predominantly rely on feed-forward networks, which may have difficulty capturing temporal correlations in time series data and identifying relevant features, resulting in less accurate predictions. Methods: Addressing this gap, we introduce novel E2E deep learning frameworks that combine Convolutional Neural Networks (CNN) and Long Short-Term Memory (LSTM) for resolving single-period inventory ordering decisions, also termed the Newsvendor Problem (NVP). This study investigates the performance drivers of hybrid CNN-LSTM architectures, coupled with an evolving algorithm for optimizing network configuration. Results: Empirical evaluation of real-world retail data demonstrates that our proposed models proficiently extract pertinent features and interpret sequential data characteristics, leading to more accurate and informed ordering decisions. Notably, results showcase substantial benefits, yielding up to an 85% reduction in costs compared to a univariate benchmark and up to 40% savings compared to a feed-forward E2E deep learning architecture. Conclusions: This confirms that, in practical scenarios, understanding the impact of features on demand empowers decision-makers to derive tailored, cost-effective ordering decisions for each store or product category.
Individualized Prediction of Blood Glucose Outcomes Using Compositional Data Analysis
Authors: Cabrera Alvis , Estremera Ernesto , Beneyto Aleix , Biagi Lyvia , Contreras Iván , Martín-Fernández Josep Antoni , Vehí Josep
Published: November 2023

This paper presents an individualized multiple linear regression model based on compositional data where we predict the mean and coefficient of variation of blood glucose in individuals with type 1 diabetes for the long-term (2 and 4 h). From these predictions, we estimate the minimum and maximum glucose values to provide future glycemic status. The proposed methodology has been validated using a dataset of 226 real adult patients with type 1 diabetes (Replace BG (NCT02258373)). The obtained results show a median balanced accuracy and sensitivity of over 90% and 80%, respectively. A information system has been implemented and validated to update patients on their glycemic status and associated risks for the next few hours.
Development of Mathematical Models for Industrial Processes Using Dynamic Neural Networks
Authors: Herceg Srečko , Ujević Andrijić Željka , Rimac Nikola , Bolf Nenad
Published: November 2023

Dynamic neural networks (DNNs) are a type of artificial neural network (ANN) designed to work with sequential data where context in time is important. Unlike traditional static neural networks that process data in a fixed order, dynamic neural networks use information about past inputs, which is important if the dynamic of a certain process is emphasized. They are commonly used in natural language processing, speech recognition, and time series prediction. In industrial processes, their use is interesting for the prediction of difficult-to-measure process variables. In an industrial isomerization process, it is crucial to measure the quality attributes that affect the octane number of gasoline. Process analyzers commonly used for this purpose are expensive and subject to failure. Therefore, to achieve continuous production in the event of a malfunction, mathematical models for estimating product quality attributes are imposed as a solution. In this paper, mathematical models were developed using dynamic recurrent neural networks (RNNs), i.e., their subtype of a long short-term memory (LSTM) architecture. The results of the developed models were compared with the results of several types of other data-driven models developed for an isomerization process, such as multilayer perceptron (MLP) artificial neural networks, support vector machines (SVM), and dynamic polynomial models. The obtained results are satisfactory, suggesting a good possibility of application.
Guardians of the Herd: Molecular Surveillance of Tick Vectors Uncovers Theileriosis Perils in Large Ruminants
Authors: Sajid Muhammad Sohail , Iqbal Asif , Rizwan Hafiz Muhammad , Kausar Asma , Tahir Urfa Bin , Younus Muhammad , Maqbool Mahvish , Siddique Rao Muhammad , Fouad Dalia , Ataya Farid Shokry
Published: November 2023

Theileria sp. (Piroplasmida: Theileriidae) is one of the most widely known infections transmitted by hard ticks (Acari: Ixodidae) and has been linked to significant economic losses across the globe. The study’s main emphasis was theileriosis, a disease that is common in Pakistan and has an incidence ranging from 0.6% to 33%. Through DNA screening of the vector ticks and host blood, this study sought to determine the risk of tick-borne theileriosis in populations of buffalos (Bubalus bubalis) and cattle (Bos indicus) in Toba Tek Singh district of Punjab, Pakistan. Identified tick species include Hyalomma anatolicum (35.4%), Rhipicephalus (Boophilus) microplus (30.2%), and R. sanguineus (25%). Tick specimens were collected from animals and their respective microenvironments. PCR assays targeting Theileria annulata were used to investigate the infection in the DNA extracted from the collected blood samples from large ruminants and salivary glands (SGs) of the Hyalomma ticks. The 18S rRNA of T. annulata was amplified using specific primers. Positive T. annulata amplicons were sequenced and verified using BLAST analysis. Overall, 50% of SGs contained T. annulate DNA. Female ticks, and those collected from cattle and from riverine environments had significantly higher (p < 0.05) rates of Theileria infection in their acini. Overall prevalence of Theileria infection was 35.9% in blood collected from large ruminants. Cattle had a substantially greater frequency of bovine theileriosis (43.2%) than buffalos (28.7%). Age and sex of large ruminants were significantly positively associated (p < 0.05) with Theileria infection. Furthermore, compared to non-riverine cattle (35%) and buffalo (19.5%), riverine cattle (52.2%) and buffalo (36.2%) showed a considerably higher prevalence. The results of this study, which is the first in Pakistan to examine the blood of large ruminants and vectorial function of Ixodid ticks in the transmission of T. annulata along with associated risk factors, offer an important insight for risk assessment of Theileria infection in livestock using vectorial infectivity.
The Dynamic Changes in the Composition and Diversity of Vaginal Microbiota in Women of Different Pregnancy Periods
Authors: Hu Feifei , Sun Xin , Su Yao , Huang Mingli
Published: November 2023

The vaginal microbiota undergoes subtle changes during pregnancy, which may affect different pregnancy responses. This study used the Illumina MiSeq high-throughput sequencing method to analyze the 16S rRNA gene amplicons of pregnant women and the vaginal microbiota structure of pregnant women at different pregnancy periods. There were a total of 15 pregnant women, with 45 samples were taken from these women, within half a year before becoming pregnant, in the last trimester, and 42 days postpartum. Before and after pregnancy, the female vaginal microbiota was mainly composed of Firmicutes, followed by Actinobacteriota and Proteobacteria. The abundance of Lactobacillus was relatively high. The α-diversity and microbial abundance were relatively low, and there was no significant difference in microbial composition between the two. After childbirth, the diversity and abundance of women’s vaginal bacterial communities were higher, with a decrease in the number of Firmicutes and a higher abundance of Actinobacteria, Proteobacteria, and Bacteroidota. There was a significant difference in the microbial community structure before and after pregnancy. This study showed that the microbiota structure of the vagina of pregnant women was similar to before pregnancy, but after childbirth, there were significant changes in the microbiota of the vagina, with a decrease in the number of probiotics and an increase in the number of harmful bacteria, increasing the risk of illness.
A First in Human Clinical Trial Assessing the Safety and Immunogenicity of Two Intradermally Delivered Enterotoxigenic Escherichia coli CFA/I Fimbrial Tip Adhesin Antigens with and without Heat-Labile Enterotoxin with Mutation LT(R192G)
Authors: Gutiérrez Ramiro L. , Riddle Mark S. , Porter Chad K. , Maciel Milton , Poole Steven T. , Laird Renee M. , Lane Michelle , Turiansky George W. , Jarell Abel , Savarino Stephen J.
Published: November 2023

Introduction: Enterotoxigenic E. coli (ETEC) is a leading cause of diarrhea in travelers as well as for children living in low- to middle-income countries. ETEC adhere to intestinal epithelium via colonization factors (CFs). CFA/I, a common CF, is composed of a polymeric stalk and a tip-localized minor adhesive subunit, CfaE. Vaccine delivery by the transcutaneous immunization of dscCfaE was safe but was poorly immunogenic in a phase 1 trial when administered to volunteers with LTR(192G) and mLT. To potentially enhance the immunogenicity of CfaE while still delivering via a cutaneous route, we evaluated the safety and immunogenicity of two CfaE constructs administered intradermally (ID) with or without mLT. Methods: CfaE was evaluated as a donor strand-complemented construct (dscCfaE) and as a chimeric construct (Chimera) in which dscCfaE replaces the A1 domain of the cholera toxin A subunit and assembles non-covalently with the pentamer of heat-labile toxin B (LTB). Subjects received three ID vaccinations three weeks apart with either dscCfaE (1, 5, and 25 µg) or Chimera (2.6 and 12.9 µg) with and without 0.1 µg of mLT. Subjects were monitored for local and systemic adverse events. Immunogenicity was evaluated by serum and antibody-secreting cell (ASC) responses. Results. The vaccine was well-tolerated with predominantly mild and moderate local vaccine site reactions characterized by erythema, induration and post-inflammatory hyperpigmentation. High rates of serologic and ASC responses were seen across study groups with the most robust responses observed in subjects receiving 25 µg of dscCfaE with 0.1 mcg of LT(R192G). Conclusion: Both ETEC adhesin vaccine prototypes were safe and immunogenic when co-administered with mLT by the ID route. The observed immune responses induced with the high dose of dscCfaE and mLT warrant further assessment in a controlled human infection model.
Gamma-Aminobutyric Acid Accumulation Contributes to Citrus sinensis Response against ‘Candidatus Liberibacter Asiaticus’ via Modulation of Multiple Metabolic Pathways and Redox Status
Authors: Nehela Yasser , Killiny Nabil
Published: November 2023

Huanglongbing (HLB; also known as citrus greening) is the most destructive bacterial disease of citrus worldwide with no known sustainable cure yet. Herein, we used non-targeted metabolomics and transcriptomics to prove that γ-aminobutyric acid (GABA) accumulation might influence the homeostasis of several metabolic pathways, as well as antioxidant defense machinery, and their metabolism-related genes. Overall, 41 metabolites were detected in ‘Valencia’ sweet orange (Citrus sinensis) leaf extract including 19 proteinogenic amino acids (PAA), 10 organic acids, 5 fatty acids, and 9 other amines (four phenolic amines and three non-PAA). Exogenous GABA application increased most PAA in healthy (except L-threonine, L-glutamine, L-glutamic acid, and L-methionine) and ‘Candidatus L. asiaticus’-infected citrus plants (with no exception). Moreover, GABA accumulation significantly induced L-tryptophan, L-phenylalanine, and α-linolenic acid, the main precursors of auxins, salicylic acid (SA), and jasmonic acid (JA), respectively. Furthermore, GABA supplementation upregulated most, if not all, of amino acids, phenolic amines, phytohormone metabolism-related, and GABA shunt-associated genes in both healthy and ‘Ca. L. asiaticus’-infected leaves. Moreover, although ‘Ca. L. asiaticus’ induced the accumulation of H2O2 and O2•− and generated strong oxidative stress in infected leaves, GABA possibly stimulates the activation of a multilayered antioxidative system to neutralize the deleterious effect of reactive oxygen species (ROS) and maintain redox status within infected leaves. This complex system comprises two major components: (i) the enzymatic antioxidant defense machinery (six POXs, four SODs, and CAT) that serves as the front line in antioxidant defenses, and (ii) the non-enzymatic antioxidant defense machinery (phenolic acids and phenolic amines) that works as a second defense line against ‘Ca. L. asiaticus’-induced ROS in citrus infected leaves. Collectively, our findings suggest that GABA might be a promising alternative eco-friendly strategy that helps citrus trees battle HLB particularly, and other diseases in general.
Proteome-Wide Profiling Using Sample Multiplexing of a Human Cell Line Treated with Cannabidiol (CBD) and Tetrahydrocannabinol (THC)
Authors: Abyadeh Morteza , Gupta Vivek , Liu Xinyue , Rossio Valentina , Mirzaei Mehdi , Cornish Jennifer , Paulo Joao A. , Haynes Paul A.
Published: November 2023

Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein–protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies.
Young Educators’ Voices on Interfaith Dialogue and Religious Diversity in Leisure Time Education: Towards an Effective Policy Framework and Training
Authors: Essomba Miquel Àngel , Nadeu Maria , Tarrés Vallespí Anna
Published: November 2023

This paper examines a study that aimed to fill in the gap in research about interfaith dialogue and leisure time education in Catalonia (Spain). Combining both quantitative and qualitative research methods, we analyzed the role that interfaith dialogue plays for leisure time educators. The research was carried out between 2021 and 2022 in three phases: one descriptive through an ad hoc questionnaire, a second one comprehensive by exploring educators’ voices in focus groups, and a third one prescriptive to design evidence-based policy recommendations and training frames. The whole research allowed us to state the low status that interfaith dialogue plays within the leisure time education in the research context, as well as the high expectations and positive attitude by educators to improve interfaith dialogue in these educational settings. Further research considering more voices (children, families, administration, religious groups) could be explored in the future to increase the knowledge on the subject.
Grouped Multilayer Practical Byzantine Fault Tolerance Algorithm: A Practical Byzantine Fault Tolerance Consensus Algorithm Optimized for Digital Asset Trading Scenarios
Authors: Liu Jian , Feng Wenlong , Huang Mengxing , Feng Siling , Zhang Yu
Published: November 2023

Based on the practical Byzantine fault tolerance algorithm (PBFT), a grouped multilayer PBFT consensus algorithm (GM-PBFT) is proposed to be applied to digital asset transactions in view of the problems with excessive communication complexity and low consensus efficiency found in the current consensus mechanism for digital asset transactions. Firstly, the transaction nodes are grouped by type, and each group can handle different types of consensus requests at the same time, which improves the consensus efficiency as well as the accuracy of digital asset transactions. Second, the group develops techniques like validation, auditing, and re-election to enhance Byzantine fault tolerance by thwarting malicious node attacks. This supervisory mechanism is implemented through the Raft consensus algorithm. Finally, the consensus is stratified for the nodes in the group, and the consensus nodes in the upper layer recursively send consensus requests to the lower layer until the consensus request reaches the end layer to ensure the consistency of the block ledger in the group. Based on the results of the experiment, the approach may significantly outperform the PBFT consensus algorithm when it comes to accuracy, efficiency, and preserving the security and reliability of transactions in large-scale network node digital transaction situations.
Researching Homosexuality in Difficult Contexts
Authors: Lyonga Frida
Published: November 2023

Homosexuality is a sensitive issue in Africa that inspires a great deal of public contention and controversy and attracts much social science research interest. Due to the sensitivity of the issue, conducting empirical research on homosexuality in Africa or within African population groups could be subject to several challenges. This article presents an autoethnographic account of my experiences conducting empirical research on attitudes towards homosexuality among Cameroonians based at home and those living in Switzerland. The paper highlights the key challenges, surprises, and lessons learned experienced in the different stages of the research process, from design to data collection, analysis, and publication of findings. Drawing on these experiences, the article calls attention to some aspects that young researchers embarking on research in sensitive topics should be aware of and should plan for upfront. It proposes practical coping approaches that can support young researchers to navigate the difficult waters of researching homosexuality in challenging settings so they can achieve their research goals within the timeframe and resources available to them.
Statistical Analysis and Health Risk Assessment: Vegetables Irrigated with Wastewater in Kirri Shamozai, Pakistan
Authors: Khan Mehak Nawaz , Aslam Muhammad Anis , Zada Imran , Albekairi Thamer H.
Published: November 2023

One of the primary environmental routes through which humans are exposed to metals and may be exposed to health risks is the food chain’s contamination with heavy metals. The study observed the risks posed by contaminants in vegetables produced in soil that received wastewater irrigation, as well as their origins and the human health impacts. Eight harmful metals (Cu, Fe, Zn, Mn, Pb, Cd, Ni, and Cr) were tested for concentration levels in water, soil, and vegetable samples using analytical techniques and an atomic absorption spectrophotometer. The present study investigated the potential health implications associated with the consumption of vegetables irrigated using wastewater containing heavy metals. The results indicated a notable accumulation of heavy metals in plant and soil samples obtained from Kirri Shamozai, Pakistan. In comparison to vegetables cultivated in soil irrigated with fresh water, the concentration levels of heavy metals in vegetables grown on soil irrigated with untreated wastewater were considerably higher at (P ≤ 0.001) and above the World Health Organization (WHO) recommended limits. The results showed that heavy metals had significantly accumulated in the soil and had permeated into the crops. Heavy metal concentrations in vegetables cultivated on land irrigated with wastewater were more significant than those grown on land irrigated with freshwater. They exceeded US EPA and World Health Organization (WHO) limits. PCA results for Pb, Cu, and Cr are the main issues impacting water quality and health hazards. The PCA results show that the soil has an extensive loading of heavy metals Cd, Ni, and Mn.
Tick-Borne Encephalitis Virus Vaccination among Tourists in a High-Prevalence Area (Italy, 2023): A Cross-Sectional Study
Authors: Riccò Matteo , Corrado Silvia , Marchesi Federico , Bottazzoli Marco
Published: November 2023

Tick-borne encephalitis (TBE) represents a potential health threat for tourists in high-risk areas, including the Dolomite Mountains in northeastern Italy. The present questionnaire-based survey was, therefore, designed in order to assess knowledge, attitudes, and preventive practices (KAP) in a convenience sample of Italian tourists visiting the Dolomite Mountains, who were recruited through online discussion groups. A total of 942 participants (39.2% males, with 60.2% aged under 50) filled in the anonymous survey from 28 March 2023 to 20 June 2023. Overall, 24.1% of participants were vaccinated against TBE; 13.8% claimed to have previously had tick bites, but no cases of TBE were reported. The general understanding of TBE was relatively low; while 79.9% of participants acknowledged TBE as a potentially severe disease, its occurrence was acknowledged as high/rather high or very high in the Dolomites area by only 51.6% of respondents. Factors associated with the TBE vaccine were assessed by the calculation of adjusted odds ratios (aOR) and 95% confidence intervals through a logistic regression analysis model. Living in areas considered at high risk for TBE (aOR 3.010, 95%CI 2.062–4.394), better knowledge on tick-borne disorders (aOR 1.515, 95%CI 1.071–2.142), high risk perception regarding tick-borne infections (aOR 2.566, 95%CI 1.806–3.646), a favorable attitude toward vaccinations (aOR 3.824, 95%CI 1.774–8.224), and a tick bite(s) in a previous season (aOR 5.479, 95%CI 3.582–8.382) were characterized as being positively associated with TBE vaccination uptake. Conversely, being <50 years old (aOR 0.646, 95%CI, 0.458–0.913) and with a higher risk perception regarding the TBE vaccine (aOR 0.541, 95%CI 0.379–0.772) were identified as the main barriers to vaccination. In summary, tourists to the high-risk area of the Dolomites largely underestimate the potential occurrence of TBE. Even though the uptake of the TBE vaccine in this research was in line with European data, public health communication on TBE is required in order to improve acceptance of this effective preventive option.
Self−Assembling Nanovaccine Fused with Flagellin Enhances Protective Effect against Foot−and−Mouth Disease Virus
Authors: Pei Chenchen , Dong Hu , Teng Zhidong , Wei Sumin , Zhang Yun , Yin Shuanghui , Tang Jianli , Sun Shiqi , Guo Huichen
Published: November 2023

Nanovaccines based on self−assembling nanoparticles (NPs) can show conformational epitopes of antigens and they have high immunogenicity. In addition, flagellin, as a biological immune enhancer, can be fused with an antigen to considerably enhance the immune effect of antigens. In improving the immunogenicity and stability of a foot−and−mouth disease virus (FMDV) antigen, novel FMDV NP antigens were prepared by covalently coupling the VP1 protein and truncated flagellin containing only N−terminus D0 and D1 (N−terminal aa 1–99, nFLiC) with self−assembling NPs (i301). The results showed that the fusion proteins VP1−i301 and VP1−i301−nFLiC can assemble into NPs with high thermal tolerance and stability, obtain high cell uptake efficiency, and upregulate marker molecules and immune−stimulating cytokines in vitro. In addition, compared with monomeric VP1 antigen, high−level cytokines were stimulated with VP1−i301 and VP1−i301−nFLiC nanovaccines in guinea pigs, to provide clinical protection against viral infection comparable to an inactivated vaccine. This study provides new insight for the development of a novel FMD vaccine.
A Systematic Review and Meta-Analysis of Anti-Rheumatic Drugs and Pneumococcal Vaccine Immunogenicity in Inflammatory Arthritis
Authors: Nagra Deepak , Bechman Katie , Adas Maryam , Yang Zijing , Alveyn Edward , Subesinghe Sujith , Rutherford Andrew , Allen Victoria , Patel Samir , Russell Mark D. , Cope Andrew , Norton Sam , Galloway James
Published: November 2023

Background: Pneumococcal pneumonia is an important cause of morbidity and mortality amongst patients with inflammatory arthritis. Vaccination is recommended by the National Institute for Health and Care Excellence (NICE) but it remains unclear how vaccine efficacy is impacted by different immunosuppressive agents. Our objective was to compare the chance of a seroconversion following vaccination against pneumococcus in patients with inflammatory arthritis to that in the general population, as well as to compare the chance of seroconversion across different targeted therapies. Methods: We searched MEDLINE, Embase and the Cochrane Library databases from inception until 20 June 2023. We included randomized controlled trials and observational studies. Aggregate data were used to undertake a pairwise meta-analysis. Our primary outcome of interest was vaccine seroconversion. We accepted the definition of serological response reported by the authors of each study. Results: Twenty studies were identified in the systematic review (2807 patients) with ten reporting sufficient data to be included in the meta-analysis (1443 patients). The chance of seroconversion in patients receiving targeted therapies, relative to the general population, was 0.61 (95% CI 0.35 to 1.08). The reduced odds of response were skewed strongly by the effects of abatacept and rituximab with no difference between patients on TNF inhibitors (TNFis) or IL-6 inhibition and healthy controls. Within different inflammatory arthritis populations the findings remained consistent, with rituximab having the strongest negative impact on vaccine response. TNF inhibition monotherapy was associated with a greater chance of vaccine response compared with methotrexate (2.25 (95% CI 1.28 to 3.96)). JAK inhibitor (JAKi) studies were few in number and did not present comparable vaccine response endpoints to include in the meta-analysis. The information available does not suggest any significant detrimental effects of JAKi on vaccine response. Conclusion: This updated meta-analysis confirms that, for most patients with inflammatory arthritis, pneumococcal vaccine can be administered with confidence and that it will achieve comparable seroconversion rates to the healthy population. Patients on rituximab were the group least likely to achieve a response and further research is needed to explore the value of multiple-course pneumococcal vaccination schedules in this population.
Influence of Sex and a High-Fiber Diet on the Gut Microbiome of Alentejano Pigs Raised to Heavy Weights
Authors: Albuquerque André , Garrido Nicolás , Charneca Rui , Egas Conceição , Martin Luísa , Ramos Amélia , Costa Filipa , Marmelo Carla , Martins José Manuel
Published: November 2023

This study investigates the influence of sex and a dietary transition on the gut microbiota of a local Portuguese pig breed. Three groups of male Alentejano pigs (n = 10 each) were raised between ~40 and 160 kg LW. Group C included pigs that were surgically castrated, while the I group included intact ones; both were fed with commercial diets. The third group, IExp, included intact pigs that were fed commercial diets until ~130 kg, then replaced by an experimental diet based on legumes and agro-industrial by-products between ~130 and 160 kg. Fecal samples were collected two weeks before slaughter. The total DNA was extracted and used for 16S metabarcoding on a MiSeq® System. The dietary transition from a commercial diet to the experimental diet substantially increased and shifted the diversity observed. Complex carbohydrate fermenting bacteria, such as Ruminococcus spp. and Sphaerochaeta spp., were significantly more abundant in IExp (q < 0.05). On the other hand, castrated pigs presented a significantly lower abundance of the potential probiotic, Roseburia spp. and Lachnospiraceae NK4A136 group (q < 0.01), bacteria commonly associated with better gut health and lower body fat composition. Understanding the role of gut microbiota is paramount to ensure a low skatole deposition and consumers’ acceptance of pork products from non-castrated male pigs.
Annotated Survey on the Research Progress within Vehicle-to-Grid Techniques Based on CiteSpace Statistical Result
Authors: Shi Ruifeng , Peng Shuaikang , Chang Tai , Lee Kwang Y.
Published: November 2023

Vehicle-to-grid (V2G) technology has received a lot of attention as a smart interconnection solution between electric vehicles and the grid. This paper analyzes the relevant research progress and hotpots of V2G by using CiteSpace 6.1.R6 software to construct a visualization graph, which includes keyword co-occurrence, clustering, and burstiness, and further systematically summarizes the main trends and key results of V2G research. First, the connection between electric vehicles and the grid is outlined and the potential advantages of V2G technology are emphasized, such as energy management, load balancing, and environmental sustainability. The important topics of V2G, including renewable energy consumption, power dispatch, regulation and optimization of the grid, and the smart grid, are discussed. This paper also emphasizes the positive impacts of V2G technologies on the grid, including reduced carbon emissions, improved grid reliability, and the support for renewable energy integration. Current and future challenges for V2G research, such as standardization, policy support, and business models, are also considered. This review provides a comprehensive perspective for scholars and practitioners in V2G research and contributes to a better understanding of the current status and future trends of V2G technology.
TOURISM DESTINATION GOVERNANCE PLAN IN THE REGION CIWIDEY, BANDUNG DISTRICT
Authors: Maldini Muhammad Rizal , R. Wisnu Rahtomo  , Daeng Noerjamal
Published: November 2023

Indonesian development in tourism is an important task of the various stakeholders involved, it is beneficial for all stakeholders because it can grow the community's economy, tourist visits to destinations, valuable tourist experiences, and many others. The management of tourism destinations in an area/destination is very much needed by the stakeholders/stakeholders so that tourism development can be sustainable. One of the areas designated as a Tourism Area is the Ciwidey Area of Bandung Regency which has districts namely Pasir Jambu District, Ciwidey District, and Rancabali District. In this study, the writer took one of the Rancabali Districts, because as many as 35% of the tourism objects in Bandung Regency were located in the Rancabali District and tourists who frequently visited the Ciwidey Region were in the Rancabali Districts. This study uses a Destination Management Organization (DMO) concept pattern which basically can directly involve stakeholders in the management of destinations in a tourism area. In this case, destination governance is not just planning but implementation must be carried out by the stakeholders involved. The concept used regarding stakeholders related to destination governance in this study uses the concept of the level of importance and influence of stakeholders and stakeholder involvement/involvement in destination governance that can improve coordination, communication, and participation between stakeholders. The problems that occur in the Rancabali Region are about tourism objects that have not been well managed, human resources that are utilized for governance are not optimal, there are still stakeholders running each, and coordination and stakeholder involvement are still not optimal. Therefore an organization's management of tourism development must go in one direction and cooperate among stakeholders
La meilleure formation consiste à réfléchir sur sa pratique
Authors: Rouxel Marie Thérèse
Published: November 2023

Solidarité Français Migrants (1) est une Association type loi 1901. Créée en novembre 1975, elle est agréée d’Education Populaire.

Ses buts sont d’établir une compréhension et une communication véritables entre français et immigrés. Elle vise à une éducation à la responsabilité, et cherche à promouvoir l’égalité des chances et des droits. Elle touche un public de femmes et d’enfants, mais atteint aussi des familles par le biais des actions de quartier et par l’action «vacances familiales ».

Depuis août 1976, elle est subventionnée par le F.A.S.

S.F.M. assure une formation continue pour tous ses membres, bénévoles et salariés, en matière d’animation et de formation d’adultes.
HIRADC Analysis for Rolling Stock Body Lifting Ngrombo Railway Maintenance Center
Authors: Riyanta Wawan
Published: November 2023

The Railway Maintenance Center as stated in the Regulation has the duty and function of carrying out the maintenance of state-owned railway facilities. The part of rolling stock heavy maintenance with high potential hazard is Lifting Body Activities Using OHTC in Maintenance of Hydraulic Diesel Locomotive Facilities activity. it so there is a need for a risk assessment which is a process to determine control priorities for the level of risk work accident or occupational disease. Part of  OHSAS 18001:2007 Clouse 4.3.1. recommendation HIRADC methode for can support a safety system with 3 stage analisys (hazard identification, risk assessment, and control determination) The Hazard Identification  results obtained 3 aspects of hazard risk in OHTC Rolling stock Body Lifting operations with the following analysis results Crane toppling, Equipment failure, Load falling with Death & asset / property damage impact factor. Risk Assessment analisys of Rolling stock Body Lifting using OHTC obtained results action taken when resources are available regarding handling which in general were Administrative Control. Determine Control relevant steps to minimize potential hazards immediately (1) Evaluating SOP Rolling stock Body Lifting and conduct outreach to all employees related to the work, (2) Making and or adding hazard signs and (3) Human development by conducting Training/Refreshment Crane Operator and Signal man. (4) Strictness of the rules for using PPE for all employees related to the work as the last step in minimizing the risk of occupational hazards.
Transformasi Cerita Rakyat "Asal-Usul Hayam Pelung" ke dalam Cerita Bergambar
Authors: Ningsih Dissa Sri Nurlaili , Maryam Siti , Adawiyah Aprilla
Published: November 2023

This article describes the process of transforming the folk tale "Asal-usul Hayam Pelung” into a pictorial story, so that folk tales in oral form are more attractive to the younger generation. The research was carried out descriptively qualitatively, with data collection techniques through library studies. The transformation process was carried out through internal changes. drama scripts and scripts into pictures. At the stage of transformation into pictures, the results are only modified character elements, while the plot, setting and scenes remain the same. Modifications are in the form of changing character elements, namely the addition of three characters (Kang Ahsan, Abdul, and Ustaz Rakhmat ). Illustrating the characters, giving rise to 5 characters, namely Mama Djarkasih, Kang Ahsan, Abdul, Ustaz Rakhmat, and Ayam Pelung. The results of the transformation of the illustrated story. “Asal-usul Hayam Pelung” from the story underwent a change in concept, namely images and text content. This illustrated story can also be used as a alternative teaching media.Keywodrs: Hayam Pelung; folklore; comic; transformationAbstrakArtikel ini mendeskripsikan proses transformasi cerita rakyat Asal-usul Hayam Pelung ke dalam cerita bergambar, sehingga cerita rakyat dalam bentuk lisan lebih diminati generasi muda. Penelitian dilakukan secara kualitatif deskriptif, dengan teknik pengumpulan data melalui studi pustaka. Proses transformasi  dilakukan melalui perubahan ke dalam naskah drama dan naskah ke dalam gambar. Pada tahap transformasi ke dalam gambar, diperoleh hasil modifikasi unsur tokoh saja, sementara alur, latar, dan adegan tetap sama. Modifikasi berupa pengubahan unsur tokoh yaitu penambahan tiga tokoh (Kang Ahsan, Abdul, dan Ustaz Rakhmat). Pengilustrasian tokoh, memunculkan 5 tokoh yaitu mama Djarkasih, Kang Ahsan, Abdul, Ustaz Rakhmat, dan ayam pelung.  Hasil transformasi  cerita bergambar Asal-Usul Ayam Pelung dari cerita mengalami perubahan konsep yaitu gambar dan isi teks. Cerita bergambar ini juga dapat dijadikan alternatif media ajar.Kata Kunci: Ayam Pelung; cerita rakyat; cerita bergambar; transformasi
Interferensi Bahasa Mandailing dalam Pemerolehan Bahasa Indonesia
Authors: Habibi M. , Iskandar Puguh Ardianto , Chandra Chandra , Suriani Ari
Published: November 2023

This article will explain the Mandailing language interference in Indonesian that occurs among elementary school children in the South Tapanuli area, North Sumatra. The research uses a qualitative approach with a case study type of research. The results of the research show that there have been 8 cases of language interference at the phonological level, 12 cases of interference at the morphological level, 20 cases of interference at the lexicon level, and 7 cases of interference at the syntactic level. The causes of interference are bilingualism, limited vocabulary, rarely used second language vocabulary and carryover of mother tongue habits. The results of this research are useful for teachers in creating a learning environment that familiarizes themselves with the use of a second language, namely Indonesian.Keywodrs: interference; Mandaling; language acquisitionAbstrakArtikel ini akan memaparkan interferensi bahasa Mandailing dalam bahasa Indonesia yang terjadi pada anak SD di daerah Tapanuli Selatan, Sumatera Utara. Penelitian menggunakan pendekatan kualitatif dengan jenis penelitian studi kasus Hasil penelitian menunjukkan telah terjadi interferensi bahasa pada tataran fonologi sebanyak 8 kasus, interferensi pada tataran morfologi sebanyak 12 kasus, interferensi pada tataran leksikon sebanyak 20 kasus, dan interferensi pada tataran sintaksis sebanyak 7 kasus. Penyebab terjadinya interferensi adalah kedwibahasaan, perbendaharaan kosakata yang masih minim, kosakata bahasa kedua jarang digunakan, dan terbawanya kebiasaan bahasa ibu. Hasil penelitian ini bermanfaat bagi guru dalam menciptakan lingkungan belajar yang membiasakan penggunaan bahasa kedua, yaitu bahasa Indonesia.Kata kunci: interferensi;  Mandaling; pemerolehan bahasa