bluuebunny/crossref_metadata_2025 huggingface dataset:
Radial Variation of Wood Anatomical Characteristics and Maturation Ages of Six Korean Oak Species
Authors: Savero Alvin Muhammad , Kim Jong-Ho , Purusatama Byantara Darsan , Prasetia Denni , Wahyudi Imam , Iswanto Apri Heri , Lee Seung-Hwan , Kim Nam-Hun
Published: February 2024

The objective of this study was to examine and compare radial variation of the anatomical characteristics and the transition age from juvenile to mature wood of the six Korean oak wood species: Quercus variabilis (Qv), Quercus serrata (Qs), Quercus mongolica (Qm), Quercus dentata (Qd), Quercus aliena (Qal), and Quercus acutissima (Qac). Quantitative anatomical features were observed from the pith to the bark at five growth-ring intervals using optical microscopy. A segmented regression model was used to evaluate the transition from juvenile wood to mature wood. The clearest transition from juvenile to mature wood was observed in the radial variation of the earlywood vessel diameter and fiber length. The maturation age of the six Korean oak species ranged from 19 to 44 years. Qv exhibited the highest values for latewood vessel diameter, fiber length, and fiber diameter. Qac displayed the highest values for earlywood vessel diameter and fiber wall thickness. The highest fiber lumen diameter was observed for Qm. The differences in earlywood and latewood vessel diameters and fiber lengths could be utilized as identification keys for these species. Earlywood vessel diameter and fiber length have emerged as the most reliable indicators for estimating the transition from juvenile to mature wood.
Differences in Response of Tree Species at Different Succession Stages to Neighborhood Competition
Authors: Zeng Xiaoxia , Li Jiejun , Peng Qiaohua , Gong Chao , Ran Huan , Xie Tingting , Liao Ting , Zhou Tianling , Huang Dongmei , Song Qingni , Liu Jun , Yang Qingpei
Published: February 2024

Neighborhood competition influences tree growth, which can affect species composition and community succession. However, there is a lack of understanding regarding how dominant tree species at different successional stages of forest communities respond in terms of crown architecture and functional traits during their growth process to neighborhood competition. In this study, we analyzed the responses of average annual basal area increment (BAI), crown architecture, and leaf functional traits of early-successional species (Cunninghamia lanceolata and Pinus massoniana), transitional species (Alniphyllum fortunei and Choerospondias axillaris), and late-successional species (Elaeocarpus duclouxii and Castanopsis carlesii) to neighbor competition in a secondary evergreen broad-leaved forest. We found that the BAI of all species is negatively correlated with competition intensity. Notably, early-successional and transitional species exhibited a more rapid decline in growth rates compared to late-successional species in response to increased competition. Among these tree species, the response of crown structure to neighbor competition exhibited variation. Early-successional and transitional species displayed a negative correlation between the competition index and crown area (CA)/diameter, while a positive correlation emerged between the lowest branch height (LBH)/height. Conversely, late-successional species followed the opposite trend. In terms of leaf functional traits, specific leaf area (SLA) showed heightened sensitivity to neighborhood competition, with a positive correlation between SLA of all tree species and the competition index. Furthermore, water use efficiency (WUE) demonstrated negative correlations with the competition index in early-successional and transitional trees, while a positive correlation emerged with late-successional trees. These findings suggest that early-successional and transitional trees prioritize vertical canopy growth, whereas late-successional trees tend to favor horizontal canopy expansion in response to neighboring competition. Additionally, early-successional and transitional trees experience more significant suppression of radial growth rate. Our research contributes to a deeper understanding of the underlying mechanisms driving changes in species composition and community succession.
Algal-Based Hollow Fiber Membrane Bioreactors for Efficient Wastewater Treatment: A Comprehensive Review
Authors: Javed Muhammad Uzair , Mukhtar Hamid , Zieniuk Bartłomiej , Rashid Umer
Published: February 2024

The treatment of living organisms is a critical aspect of various environmental and industrial applications, ranging from wastewater treatment to aquaculture. In recent years, algal-based hollow fiber membrane bioreactors (AHFMBRs) have emerged as a promising technology for the sustainable and efficient treatment of living organisms. This review provides a comprehensive examination of AHFMBRs, exploring their integration with algae and hollow fiber membrane systems for diverse applications. It also examines the applications of AHFMBRs in various areas, such as nutrient removal, wastewater treatment, bioremediation, and removal of pharmaceuticals and personal care products. The paper discusses the advantages and challenges associated with AHFMBRs, highlights their performance assessment and optimization strategies, and investigates their environmental impacts and sustainability considerations. The study emphasizes the potential of AHFMBRs in achieving enhanced nutrient removal, bioremediation, and pharmaceutical removal while also addressing important considerations such as energy consumption, resource efficiency, and ecological implications. Additionally, it identifies key challenges and offers insights into future research directions. Through a systematic analysis of relevant studies, this review aims to contribute to the understanding and advancement of algal-based hollow fiber membrane bioreactors as a viable solution for the treatment of living organisms.
Evaluation of Hydrothermally Treated Wood Fibre Performance in Cement Mortars
Authors: Kampragkou Petrini , Kamperidou Vasiliki , Stefanidou Maria
Published: February 2024

Biofibres’ wide application in mortar enhancement has thus far been restricted by factors related to their chemical composition and hygroscopic nature. Their hydrophilic behaviour increases the water demand of mortar mixtures and diminishes their affinity to the matrix, while further moisture-related fibre degradation issues may arise. Additionally, natural fibres seem to be susceptible to degradation caused by exposure to alkaline environmental conditions such as those experienced by cement mortars, restricting their utilisation in the construction industry. Therefore, the current study investigates the potential of fibre modification through treatments that would permanently alter their structure and chemical composition to improve their performance. In this study, wood fibres of black pine and beech species were exposed to mild thermal treatment (140 °C 2 h, under a steam atmosphere), characterised in terms of the physical and chemical properties and incorporated in cement mortars, applying the proportion of 1.5% v/v in the mortar, in order to assess their performance as reinforcement material. The mortars’ workability (at a fresh state) was examined, as well as other physical, hygroscopic, thermal, and mechanical characteristics of the mortars at the ages of 28, 90 and 365 days and weathering performance, by subjecting them to different artificial ageing environments (freeze–thaw cycles or outdoor exposure). The results revealed the beneficial role of the treated fibres in dimensional stability, flexural strength, thermal insulation properties and capillary absorption of the mortar specimens, especially during the ageing process, with the black pine fibres showing the greatest improvement. The hydrothermally treated wood fibres seem to help maintain the integrity of cement mortars under all ageing conditions, proving that they could provide low-cost and eco-friendly mortar enhancement pathways.
Ignition of Forest Fires by Cigarette Butts: Using Pinus massoniana Needles as an Example
Authors: Zhang Yunlin , Tian Lingling
Published: February 2024

As a cigarette butt falls onto the forest surface fuel, it first smolders the fuel, then ignites into flames, and spreads as forest fire under certain conditions. In this study, the needles under a typical stand of P. massoniana were used as the research object. Needle beds with different moisture content and packing ratios were constructed indoors. Cigarette butt-ignition experiments were conducted under different wind velocities, and 30 experiment cycles were conducted under different conditions. There was a total of 5 (packing ratio) × 4 (moisture content) × 6 (wind velocity) = 120 sets of conditions, and a total of 3600 ignition experiments were conducted. The results showed that (1) the total ignition probability of the cigarette butts was 2.36%, which only occurred when the fuelbed moisture content was <10% and the wind velocity was >1 m/s. The ignition time of cigarette butts ranged from 2.73 to 7.25 min. (2) The fuelbed moisture content and wind velocity significantly influenced the ignition probability and time. With an increase in moisture content, the ignition probability of cigarette butts decreased, while the time required for ignition showed an increasing trend. Wind velocity had a dual effect on ignition. The ignition effect was optimal at a wind velocity of 4 m/s. With an increase in wind velocity, the ignition probability first increased and then decreased, and the ignition time first decreased and then increased. (3) The packing ratio had no significant effect on the ignition probability; however, the ignition time significantly decreased as the packing ratio increased. (4) The logistic regression method (LRM), general linear method (GLM), and nonlinear regression method (NLM) were used to establish a prediction model of ignition probability. The prediction effect of GLM was the worst, followed by LRM, and the NLM had the best prediction effect. The GLM was selected to establish the ignition time model, and the error was also within the allowance range. This study elucidated the underlying mechanism of factors affecting cigarette butt-based fuel ignition. In addition, the established prediction model provides a reference for human-caused forest fires and is highly significant for forest fire prevention.
Improved Generation, Physicochemical Characteristics, and Food Application Studies of a Red Colorant Obtained from Oxidative Coupling of Chlorogenic Acid and Tryptophan
Authors: Santarcangelo Ardemia , Schulze-Kaysers Nadine , Schieber Andreas
Published: February 2024

Due to a widespread consumer reluctance toward synthetic food dyes, the interest in natural compounds from plants has increased. This study aimed to optimize the oxidation process between chlorogenic acid (CQA) and tryptophan (Trp) using sodium periodate (NaIO4) to obtain a red-colored pigment. The impact of temperature and different ratios of Trp to NaIO4 on the reaction progress was investigated. After the best conditions for the reaction were established, three pH values were tested. The reaction time could be reduced from 72 to 24 h with a yield of 46 ± 2% w/w based on the quantity of CQA. After the first purification step of the product by size exclusion chromatography, the pigment obtained was characterized for its solubility, and its hydrolyzed form was used for investigations into the stability at different pH values, storage under light and in the dark (period of 28 days), in the presence of reducing agents, and for heat resistance. Finally, several food matrices were successfully colored with the natural pigment in amounts from 0.005 to 0.01% (w/w). In conclusion, the present study provides new insights into the feasible production and comprehensive characterization of a red pigment derived from oxidative coupling of CQA and Trp, as well as its application in food systems.
The Phase-Dependent Regulation of Lux-Type Genes on the Spoilage Characteristics of Hafnia alvei
Authors: Bi Jingran , Yao Qiaoli , Zhang Gongliang , Hou Hongman
Published: February 2024

Hafnia alvei, a specific spoilage microorganism, has a strong capacity to destroy food protein and lead to spoilage. The aim of this study was to evaluate the phase-dependent regulation of lux-type genes on the spoilage characteristics of H. alvei H4. The auto-inducer synthase gene luxI and a regulatory gene luxR of the quorum sensing systems in H. alvei H4 were knocked out to construct the mutant phenotypes. On this basis, the research found that the luxI and luxR genes had a strong positive influence on not only flagella-dependent swimming ability and biofilm formation but also the production of putrescine and cadaverine. The luxR gene could downregulate putrescine production. The maximum accumulation of putrescine in wild type, ΔluxI, ΔluxR and ΔluxIR were detected at 24 h, reaching up to 695.23 mg/L, 683.02 mg/L, 776.30 mg/L and 724.12 mg/L, respectively. However, the luxI and luxR genes have a potential positive impact on the production of cadaverine. The maximum concentration of cadaverine produced by wild type, ΔluxI, ΔluxR and ΔluxIR were 252.7 mg/L, 194.5 mg/L, 175.1 mg/L and 154.2 mg/L at 72 h. Moreover, the self-organizing map analysis revealed the phase-dependent effects of two genes on spoilage properties. The luxI gene played a major role in the lag phase, while the luxR gene mainly acted in the exponential and stationary phases. Therefore, the paper provides valuable insights into the spoilage mechanisms of H. alvei H4.
Effect of Damaged Starch and Wheat-Bran Arabinoxylans on Wheat Starch and Wheat Starch–Gluten Systems
Authors: Teobaldi Andrés Gustavo , Barrera Gabriela Noel , Ribotta Pablo Daniel
Published: February 2024

This study investigated the impact of damaged starch and arabinoxylans on the thermal and pasting behavior of mixtures containing starch and gluten. The mixtures containing starch, arabinoxylans, and gluten were dispersed in water and a 50% sucrose solution. When arabinoxylans were added to native starch in water, it did not modify the viscosity profiles. An increase in viscosity parameters was observed due to the addition of arabinoxylans to starch with a higher level of damage. Gluten did not influence the effects caused by arabinoxylans. In the sucrose solution, arabinoxylans caused an increase in the viscosity parameters of native starch and starch with higher damage content dispersions. Gluten caused greater viscosity increases when arabinoxylans were added. In water, the addition of arabinoxylans to native starch caused a decrease in the enthalpy of gelatinization and an increase in the onset temperature. Adding arabinoxylans to starch with a higher level of damage caused the opposite effects. In the presence of sucrose, arabinoxylans caused a decrease in the enthalpy of gelatinization. These results lay the foundations for studying the influence of damaged starch and arabinoxylans in water-rich systems characterized by the presence of substantial proportions of sucrose, such as batter formulations.
The Cardioprotective Role of Nitrate-Rich Vegetables
Authors: Olas Beata
Published: February 2024

Nitric oxide (NO) is an inorganic radical produced by both the non-enzymatic nitrate (NO3−)—nitrite (NO2−)—NO pathway and enzymatic reactions catalyzed by nitric oxide synthase (NOS). Also, as nitrate and nitrite from dietary and other endogenous sources can be reduced back to nitric oxide in vivo, the endogenous NO level can be increased through the consumption of nitrate–rich vegetables. Ingestion of dietary NO3− has beneficial effects which have been attributed to a subsequent increase in NO: a signaling molecule that may regulate various systems, including the cardiovascular system. A diet rich in NO3− from green leafy and root vegetables has cardioprotective effects, with beetroot products being particularly good sources of NO3−. For example, various studies have demonstrated a significant increase in nitrite levels (regarded as markers of NO) in plasma after the intake of beetroot juice. The present review describes the current literature concerning the role of nitrate-rich vegetables (especially beetroot products) in the prophylaxis and treatment of cardiovascular diseases (CVDs). This review is based on studies identified in electronic databases, including PubMed, ScienceDirect, Web of Knowledge, Sci Finder, Web of Science, and SCOPUS.
Volatiles from French and Croatian Sea Fennel Ecotypes: Chemical Profiles and the Antioxidant, Antimicrobial and Antiageing Activity of Essential Oils and Hydrolates
Authors: Politeo Olivera , Ćurlin Pavao , Brzović Petra , Auzende Killian , Magné Christian , Generalić Mekinić Ivana
Published: February 2024

Sea fennel is a halophytic plant rich in valuable nutritional components and is characterized by pleasant organoleptic properties. While its essential oils (EOs) are well investigated, there are no reports on the volatiles from their corresponding hydrolates, which are the main by-products of EO isolation, as well as on their biological activity. Therefore, the composition and biological activities of EOs and corresponding hydrolates of sea fennel from Atlantic (French, FRA) and Mediterranean (Croatian, CRO) ecotypes were investigated and compared. The EO from the CRO sample was characterized by an abundance of sabinene and limonene, while that from the FRA ecotype was rich in dillapiol and carvacryl methyl ether. The CRO hydrolate was rich in terpinen-4-ol and 10-(acetylmethyl)-3-carene, while dillapiol, thymyl methyl ether and γ-terpinene were the main compounds in the FRA sea fennel hydrolate. The biological activities of the EOs and hydrolates were evaluated for their antioxidant (with DPPH, NO, FRAP and ORAC bioassays), antimicrobial (against some Gram+ and Gram- spoilage bacteria) and antiageing (tyrosinase, elastase and collagenase inhibition) activities. Both EOs showed low reducing powers and antiradical activities while the ability of both hydrolates to quench NO was slightly higher (35–39% if inhibition). The FRA EO showed low activity against Staphylococcus aureus (8 mm), while CRO moderately inhibited the growth of P. aeruginosa (8 mm), but strongly inhibited the other two bacterial strains. While the French EO showed no antityrosinase and anticollagenase activity, the Croatian oil significantly inhibited both enzymes (IC50 of 650 µg/mL and IC50 of 2570 µg/mL, respectively) probably due to the dominance of limonene and sabinene. Neither EO exhibited antielastase properties, while the hydrolates from both ecotypes showed no antiageing activity, regardless of the enzyme tested. The EOs from the aerial parts of sea fennel from FRA and CRO differed greatly in composition, resulting in different activities. The Croatian samples appeared to have better biological properties and are therefore good candidates for applications as preservatives or antiageing agents.
Zein Multilayer Electrospun Nanofibers Contain Essential Oil: Release Kinetic, Functional Effectiveness, and Application to Fruit Preservation
Authors: Moradinezhad Farid , Aliabadi Majid , Ansarifar Elham
Published: February 2024

In this study, sequential electrospinning was employed to produce a multilayer film consisting of zein nanofibers (Z) and Zataria multiflora essential oil (ZMEO) with different layers. The layers include: Z (without ZMEO), Z1 (one layer of Z + ZMEO), Z3 (three layers of Z + ZMEO), and Z5 (five layers of Z + ZMEO). Then, the effect of this antimicrobial packaging was investigated in relation to increasing the shelf life of strawberries at 4 °C for 12 days. The scanning electron microscopy (SEM) images of the fibers demonstrated a uniform and smooth structure without any beads. The use of Fourier transform infrared (FTIR) and Differential scanning calorimetry (DSC) showed that ZMEO was physically encapsulated into multilayer Z, resulting in an enhancement in thermal stability. The multilayer film showed a sustained release pattern of the encapsulated ZMEO for Z3, lasting for 90 h, and Z5, lasting for 180 h. This was in contrast to the rapid release within 50 h observed with Z film. The release kinetics for Z5 showed a good correlation with both the Higuchi and Korsmeyer–Peppas models, while for Z1 and Z3 films, Fickian diffusion was identified as the underlying mechanism. The findings of this study indicated that the multilayer film released ZMEO through a combination of diffusion and polymeric erosion. During a 12-day period of cold storage, strawberries that were treated with Z5 showed significant preservation of their anthocyanin (32.99%), antioxidant activity (25.04%), weight loss (24.46%), titratable acidity (11.47%), firmness (29.67%), and color (10.17%) compared to the control sample. The findings indicated that the sequential electrospinning technique used to create the multilayer nanofibrous film could be used in various fields, such as bioactive encapsulation, controlled release, antimicrobial packaging, and food preservation.
Effect of Plasma-Activated Water (PAW) on the Postharvest Quality of Shepherd’s Purse (Capsella bursa-pastoris)
Authors: Xiong Lingming , Feng Lei , Nie Meimei , Li Dajing , Zhang Zhongyuan , Liu Chunquan , Dai Zhuqing , Xiao Yadong , Xu Yayuan
Published: February 2024

Plasma-activated water (PAW) treatment is an effective technique for the quality retention of fresh vegetables with cold atmospheric plasma using controllable parameters. This study investigated the effect of PAW on the postharvest quality of shepherd’s purse (Capsella bursa-pastoris). The results displayed that PAW treatment with an activation time of 5, 10, 15, and 20 min reduced the yellowing rate and weight loss of the shepherd’s purse during 9 days of storage. Compared with untreated samples, PAW treatment at different times reduced the number of total bacteria, coliform, yeast, and mold by 0.18–0.94, 0.59–0.97, 0.90–1.18, and 1.03–1.17 Log CFU/g after 9 days of storage, respectively. Additionally, the treatments with PAW-5 and PAW-10 better preserved ascorbic acid, chlorophyll, total phenol, and total flavonoid contents. They also maintained the higher antioxidant and CAT activity and inhibited the formation of terpenes, alcohols, and nitrogen oxide compounds of the shepherd’s purse at the end of storage. The microstructural result illustrated that the cells of the shepherd’s purse treated with PAW-5 and PAW-10 were relatively intact, with a small intercellular space after storage. This study demonstrated that PAW treatment effectively improved the postharvest quality of shepherd’s purse.
Combination of Sodium Nitroprusside and Controlled Atmosphere Maintains Postharvest Quality of Chestnuts through Enhancement of Antioxidant Capacity
Authors: Pang Linging , Jiang Yuqian , Chen Lan , Shao Chongxiao , Li Li , Wang Xiaodong , Li Xihong , Pan Yanfang
Published: February 2024

The purpose of this study was to clarify the effect of CA (controlled atmosphere, 2–3% O2 + 3% CO2) and NO (nitric oxide, generated by 0.4 nM sodium nitroprusside), alone or combined (CA + NO), on the physio-chemical properties, enzyme activities and antioxidant capacities of chestnuts during storage at 0 °C for 180 d. Compared with control (CT), CA and CA+NO both improved the storage quality of the samples, but only CA resulted in more ethanol production. Moreover, these improvements were further enhanced and ethanol synthesis was inhibited by the addition of NO. A spectrometer was used to assess the production of phenolic content (TPC) and activities of phenylalanine ammonia-lyase (PAL), superoxide dismutas (SOD), peroxidase (POD), catalase (CAT) and polyphenol oxidase (PPO) as influenced by CA or CA+NO treatments. Higher TPC, PAL, SOD, POD, CAT, and lower PPO were observed in CA alone, and more so in the combination with NO group. The increased antioxidant production and enhanced antioxidant activities contributed to scavenging reactive oxygen species (ROS) and reducing malondialdehyde (MDA). This study unveiled the correlations and differences between the effects of CA and CA+NO on storage quality, providing valuable insights into postharvest preservation and suggesting that the combination (CA+NO) was more beneficial for quality maintenance in chestnuts.
Effects of Particle Size on Physicochemical and Nutritional Properties and Antioxidant Activity of Apple and Carrot Pomaces
Authors: Salari Saeed , Ferreira Joana , Lima Ana , Sousa Isabel
Published: February 2024

The food processing industry is growing rapidly and producing large amounts of by-products, such as pomaces, which are considered as no-value waste and cause significant environmental pollution. The main by-products of fruit juice processing companies are apple and carrot pomaces, which can be used to create new functional food products. In the present study, the effects of particle size (PS) on the proximate composition, nutritional properties, and antioxidant activity of apple pomace flour (APF) and carrot pomace flour (CPF) were determined. Four different PS fractions, PS > 1 mm, 1 > PS > 0.71 mm, 0.71 > PS > 0.18 mm, and 0.18 > PS > 0.075 mm were used for the present study. Their vitamin, carotenoid, organic acid, and reducing sugar contents were determined using HPLC. The proximate compositions of each PS fraction of the AP and CP flours were determined using recommended international standard methods. DPPH, FRAP, and Folin-Ciocalteu methods were used to measure their antioxidant activity and total phenolic compounds, respectively. The moisture content (around 12.1 mg/100 g) was similar in all PS fractions and in both flours. The APF had lower protein (4.3–4.6 g/100 g dw) and ash (1.7–2.0 g/100 g dw) contents compared to the CPF, with protein contents ranging from 6.4–6.8 g/100 g dw and ash contents ranging from 5.8–6.1 g/100 g dw. Smaller particles, regardless of flour type, exhibited higher sugar and phenolic contents and antioxidant activity, while vitamins were more abundant in particles larger than 1 mm. In the APF, larger particles had a higher fiber content than smaller particles, while their fat content was the lowest. PS also had an impact on the results of the carotenoid contents. This study underscores the direct impact of PS on the distribution of sugars, crude fiber, fat, carotenoids, vitamins, total phenolic compounds, and antioxidant activity in pomaces.
Exploring the Potential of Plant-Derived Exosome-like Nanovesicle as Functional Food Components for Human Health: A Review
Authors: Liu Yizhi , Ren Chaoqin , Zhan Ruiling , Cao Yanan , Ren Yuanhang , Zou Liang , Zhou Chuang , Peng Lianxin
Published: February 2024

Plant-derived exosome-like nanovesicles (PELNs) are bilayer membrane-enclosed nanovesicles secreted by plant cells, serving as carriers of various substances such as proteins, RNA, and metabolites. The mounting evidence suggests that PELN plays a crucial role in transmembrane signaling, nutrient transportation, apoptosis, and regulation of gut microbiota composition. This makes it a promising “dark nutrient” for plants to modulate human physiology and pathogenesis. A comprehensive understanding of PELN formation, uptake, and functional mechanisms can offer novel insights into plant nutrition and functional properties, thereby facilitating the precise development of plant-based foods and drugs. This article provides a summary of PELN extraction and characterization, as well as absorption and delivery processes. Furthermore, it focuses on the latest discoveries and underlying physiological mechanisms of PELN’s functions while exploring future research directions.
Review of the Terminology, Approaches, and Formulations Used in the Guidelines on Quantitative Risk Assessment of Chemical Hazards in Food
Authors: Doménech Eva , Martorell Sebastián
Published: February 2024

This paper reviews the published terminology, mathematical models, and the possible approaches used to characterise the risk of foodborne chemical hazards, particularly pesticides, metals, mycotoxins, acrylamide, and polycyclic aromatic hydrocarbons (PAHs). The results confirmed the wide variability of the nomenclature used, e.g., 28 different ways of referencing exposure, 13 of cancer risk, or 9 of slope factor. On the other hand, a total of 16 equations were identified to formulate all the risk characterisation parameters of interest. Therefore, the present study proposes a terminology and formulation for some risk characterisation parameters based on the guidelines of international organisations and the literature review. The mathematical model used for non-genotoxic hazards is a ratio in all cases. However, the authors used the probability of cancer or different ratios, such as the margin of exposure (MOE) for genotoxic hazards. For each effect studied per hazard, the non-genotoxic effect was mostly studied in pesticides (79.73%), the genotoxic effect was mostly studied in PAHs (71.15%), and both effects were mainly studied in metals (59.4%). The authors of the works reviewed generally opted for a deterministic approach, although most of those who assessed the risk for mycotoxins or the ratio and risk for acrylamide used the probabilistic approach.
What Are the Limitations and Challenges of Swab-Based DNA Sampling?
Authors: Bruijns Brigitte
Published: February 2024

Selecting the optimal sampling method is an essential component of the DNA analysis process. Errors or omissions in targeting and gathering relevant samples can significantly reduce the likelihood of obtaining a valuable DNA profile, affecting the profile’s quality and evidential value and ultimately hindering its ability to support the justice system. While DNA typing techniques have become significantly more sensitive in recent years, there is an ongoing need for further advancements in the recovery of DNA from crime scenes. It is essential to improve the accuracy and reliability of forensic investigations, particularly in cases where only tiny amounts of DNA are present, such as touch DNA samples or degraded forensic evidence. Parameters, including swab material, type of substrate, and swabbing protocol, that influence the efficiency of a swab are discussed in this review. This is followed by a literature review of studies that have compared swab types and/or other sampling conditions. While swabs are the most-used collection tools at a crime scene, alternatives are available. These alternatives are reviewed, including their advantages and disadvantages. A critical discussion and conclusions make clear that, unfortunately, neither swabs nor their alternatives are highly effective in recovering DNA from a substrate.
A Bearing Fault Diagnosis Method under Small Sample Conditions Based on the Fractional Order Siamese Deep Residual Shrinkage Network
Authors: Li Tao , Wu Xiaoting , Luo Zhuhui , Chen Yanan , He Caichun , Ding Rongjun , Zhang Changfan , Yang Jun
Published: February 2024

A bearing fault is one of the major causes of rotating machinery faults. However, in real industrial scenarios, the harsh and complex environment makes it very difficult to collect sufficient fault data. Due to this limitation, most of the current methods cannot accurately identify the fault type in cases with limited data, so timely maintenance cannot be conducted. In order to solve this problem, a bearing fault diagnosis method based on the fractional order Siamese deep residual shrinkage network (FO-SDRSN) is proposed in this paper. After data collection, all kinds of vibration data are first converted into two-dimensional time series feature maps, and these feature maps are divided into the same or different types of fault sample pairs. Then, a Siamese network based on the deep residual shrinkage network (DRSN) is used to extract the features of the fault sample pairs, and the fault type is determined according to the features. After that, the contrastive loss function and diagnostic loss function of the sample pairs are combined, and the network parameters are continuously optimized using the fractional order momentum gradient descent method to reduce the loss function. This improves the accuracy of fault diagnosis with a small sample training dataset. Finally, four small sample datasets are used to verify the effectiveness of the proposed method. The results show that the FO-SDRSN method is superior to other advanced methods in terms of training accuracy and stability under small sample conditions.
Minimal Wave Speed for a Nonlocal Viral Infection Dynamical Model
Authors: Ren Xinzhi , Liu Lili , Zhang Tianran , Liu Xianning
Published: February 2024

To provide insights into the spreading speed and propagation dynamics of viruses within a host, in this paper, we investigate the traveling wave solutions and minimal wave speed for a degenerate viral infection dynamical model with a nonlocal dispersal operator and saturated incidence rate. It is found that the minimal wave speed c∗ is the threshold that determines the existence of traveling wave solutions. The existence of traveling fronts connecting a virus-free steady state and a positive steady state with wave speed c≥c∗ is established by using Schauder’s fixed-point theorem, limiting arguments, and the Lyapunov functional. The nonexistence of traveling fronts for c<c∗ is proven by the Laplace transform. In particular, the lower-bound estimation of the traveling wave solutions is provided by adopting a rescaling method and the comparison principle, which is a crucial prerequisite for demonstrating that the traveling semifronts connect to the positive steady state at positive infinity by using the Lyapunov method and is a challenge for some nonlocal models. Moreover, simulations show that the asymptotic spreading speed may be larger than the minimal wave speed and the spread of the virus may be postponed if the diffusion ability or diffusion radius decreases. The spreading speed may be underestimated or overestimated if local dispersal is adopted.
Nash Equilibria in Two-Resource Congestion Games with Player-Specific Payoff Functions
Authors: Khanchouche Fatima , Sbabou Samir , Smaoui Hatem , Ziad Abderrahmane
Published: February 2024

In this paper, we examine the class of congestion games with player-specific payoff functions introduced by Milchtaich, I. (1996). Focusing on the special case of two resources, we give a short and simple method for identifying all Nash equilibria in pure strategies. We also provide a computation algorithm based on our theoretical analysis.
Three-Dimensional Printing of Red Algae Biopolymers: Effect of Locust Bean Gum on Rheology and Processability
Authors: Oliveira Sónia , Sousa Isabel , Raymundo Anabela , Bengoechea Carlos
Published: February 2024

Seaweeds, rich in high-value polysaccharides with thickening/gelling properties (e.g., agar, carrageenan, and alginate), are extensively used in the food industry for texture customization and enhancement. However, conventional extraction methods for these hydrocolloids often involve potentially hazardous chemicals and long extraction times. In this study, three red seaweed species (Chondrus crispus, Gelidium Corneum, and Gracilaria gracilis) commercialized as food ingredients by local companies were chosen for their native gelling biopolymers, which were extracted using water-based methodologies (i.e., (1) hydration at room temperature; (2) stirring at 90 °C; and (3) centrifugation at 40 °C) for production of sustainable food gels. The potential use of these extracts as bioinks was assessed employing an extrusion-based 3D printer. The present work aimed to study the gelation process, taken place during printing, and assess the effectiveness of the selected green extraction method in producing gels. To improve the definition of the printed gel, two critical printing parameters were investigated: the addition of locust bean gum (LBG) at different concentrations (0, 0.5, 1, 1.5, 2, and 2.5%) and printing temperature (30, 40, 60, and 80 °C). Rheological results from a controlled-stress rheometer indicated that gels derived from G. corneum and G. gracilis exhibited a lower gel strength (lower G′ and G″) and excessive material spreading during deposition (lower viscosity) than C. crispus. Thus, G′ was around 5 and 70 times higher for C. crispus gels than for G. corneum and G. gracilis, respectively. When increasing LBG concentration (0.5 to 2.5% w/w) and lowering the printing temperature (80 to 30 °C), an enhanced gel matrix definition for G. corneum and G. gracilis gels was found. In contrast, gels from C. crispus demonstrated greater stability and were less influenced by these parameters, showcasing the potential of the seaweed to develop sustainable clean label food gels. Eventually, these results highlight the feasibility of using algal-based extracts obtained through a green procedure as bioinks where LBG was employed as a synergic ingredient.
Novel Ion Channel Genes in Malaria Parasites
Authors: Desai Sanjay A.
Published: February 2024

Ion channels serve many cellular functions including ion homeostasis, volume regulation, signaling, nutrient acquisition, and developmental progression. Although the complex life cycles of malaria parasites necessitate ion and solute flux across membranes, the whole-genome sequencing of the human pathogen Plasmodium falciparum revealed remarkably few orthologs of known ion channel genes. Contrasting with this, biochemical studies have implicated the channel-mediated flux of ions and nutritive solutes across several membranes in infected erythrocytes. Here, I review advances in the cellular and molecular biology of ion channels in malaria parasites. These studies have implicated novel parasite genes in the formation of at least two ion channels, with additional ion channels likely present in various membranes and parasite stages. Computational approaches that rely on homology to known channel genes from higher organisms will not be very helpful in identifying the molecular determinants of these activities. Given their unusual properties, novel molecular and structural features, and essential roles in pathogen survival and development, parasite channels should be promising targets for therapy development.
Tirzepatide and Glycemic Control Metrics Using Continuous Glucose Monitoring in Older Patients with Type 2 Diabetes Mellitus: An Observational Pilot Study
Authors: Omura Takuya , Inami Akemi , Sugimoto Taiki , Kawashima Shuji , Sakurai Takashi , Tokuda Haruhiko
Published: February 2024

This observational pilot study aimed to investigate continuous glucose monitoring (CGM) metrics in older Japanese patients with type 2 diabetes mellitus (T2DM) using a CGM system (FreeStyle Libre Pro) during the first tirzepatide administration and compare the glycemic control measures before and after the initial injection. The four patients had a mean age of 79.5 years (standard deviation [SD]: 5.8), a mean body mass index of 24.6 kg/m2 (SD: 4.7), a mean glycated hemoglobin level of 9.1% (SD: 2.1), and a mean measurement period of 10.5 days (SD: 3.5). After the inclusion of tirzepatide treatment, the mean of time in range, time above range, and time below range changed from 53.2% to 78.9% (p = 0.041), 45.8% to 19.7% (p = 0.038), and 1.0% to 1.5% (p = 0.206), respectively. Improved hyperglycemia reduced the oral hypoglycemic medication in two cases and decreased the frequency of insulin injections in two cases. To elucidate the potential benefits of tirzepatide, future studies should investigate the long-term impact on functional prognosis, safety, and tolerability and distinguish between the use of other weekly agonists, especially in nonobese older Asian patients. However, tirzepatide-associated robust glycemic improvement may simplify diabetes treatment regimens in older patients with T2DM.
Deprescribing in Older Poly-Treated Patients Affected with Dementia
Authors: Gareri Pietro , Gallelli Luca , Gareri Ilaria , Rania Vincenzo , Palleria Caterina , De Sarro Giovambattista
Published: February 2024

Polypharmacy is an important issue in older patients affected by dementia because they are very vulnerable to the side effects of drugs’. Between October 2021 and September 2022, we randomly assessed 205 old-aged outpatients. The study was carried out in a Center for Dementia in collaboration with a university center. The primary outcomes were: (1) deprescribing inappropriate drugs through the Beers and STOPP&START criteria; (2) assessing duplicate drugs and the risk of iatrogenic damage due to drug–drug and drug–disease interactions. Overall, 69 men and 136 women (mean age 82.7 ± 7.4 years) were assessed. Of these, 91 patients were home care patients and 114 were outpatient. The average number of the drugs used in the sample was 9.4 drugs per patient; after the first visit and the consequent deprescribing process, the average dropped to 8.7 drugs per patient (p = 0.04). Overall, 74 potentially inappropriate drugs were used (36.1%). Of these, long half-life benzodiazepines (8.8%), non-steroidal anti-inflammatory drugs (3.4%), tricyclic antidepressants (3.4%), first-generation antihistamines (1.4%), anticholinergics (11.7%), antiplatelet drugs (i.e., ticlopidine) (1.4%), prokinetics in chronic use (1.4%), digoxin (>0.125 mg/day) (1.4%), antiarrhythmics (i.e., amiodarone) (0.97%), and α-blockers (1.9%) were included. The so-called “duplicate” drugs were overall 26 (12.7%). In total, ten potentially dangerous prescriptions were found for possible interactions (4.8%). We underline the importance of checking all the drugs taken periodically and discontinuing drugs with the lowest benefit-to-harm ratio and the lowest probability of adverse reactions due to withdrawal. Computer tools and adequately trained teams (doctors, nurses, and pharmacists) could identify, treat, and prevent possible drug interactions.
An International Classification of Functioning, Disability and Health Model-Based Analysis of Suicidal Ideation among 9920 Community-Dwelling Korean Older Adults
Authors: Byeon Haewon
Published: February 2024

Background: Many complex factors contribute to suicide in older adults. The suicidal ideation that precedes suicide is an especially direct predictor of suicide. This study aimed to identify the effects between variables affecting suicidal ideation among older adults using the International Classification of Functioning, Disability and Health (ICF) model and understand the causal relationships to systematize complex factors. Methods: This study used data from 9920 community-dwelling older adults who completed a national survey in 2020 to classify predictors of suicidal ideation (e.g., depression, subjective health status, sociodemographic factors, health factors, social support, instrumental activities of daily living (IADL), and social participation) by using the ICF model. To determine the causal relationship between variables, this study examined significance based on the critical ratio (C.R.) and squared multiple correlation (SMC) by using a path model. Results: Gender, education level, economic level, age, IADL, relationship satisfaction with a child, depression, and the number of chronic diseases significantly affected suicidal ideation, while age, employment status, participation in social groups, formal and informal support, satisfaction with a friend/neighbor relationship, and subjective health status did not significantly influence it. Moreover, depression mediated the relationship between each of these variables and suicidal ideation. Conclusions: It was found that depression was the most direct and mediating factor in suicidal ideation among many factors affecting the suicidal ideation of community-dwelling older adults. Additional studies should be conducted to develop community-level strategies based on these factors and understand causal relationships.
The Prevalence of Congenitally Missing Permanent Teeth in a Sample of Orthodontic and Non-Orthodontic Caucasian Patients
Authors: Katanaki Nefeli , Makrygiannakis Miltiadis A. , Kaklamanos Eleftherios G.
Published: February 2024

Background: Hypodontia represents a notable clinical and public health concern. Objective: To assess the prevalence of congenitally missing permanent teeth in a sample of orthodontic/dental patients of Caucasian origin originating from the Greek island of Lesvos. Materials and Methods: Panoramic X-rays from 621 children and adolescents, aged 9 to 16 years (average age 12.5 years), 521 seeking orthodontic care (orthodontic group) and 100 seeking dental care (non-orthodontic group) were examined to identify congenitally missing permanent teeth. Results: The orthodontic group exhibited a 5.5% prevalence of congenitally missing permanent teeth (2.8% females; 2.7% males), while the non-orthodontic group showed a prevalence of 4% (3% females; 1% males). The descending order of prevalence for missing tooth types was as follows: lower second premolars, upper laterals, lower central incisors, lower canines, upper second premolars, and lower second molars. Among orthodontic patients with missing teeth, 62% presented with an Angle’s Class II malocclusion. Hypodontia was most frequently observed in the mandible. No statistically significant differences were observed between the orthodontic and non-orthodontic groups in terms of the percentage of children and types of congenitally missing teeth. Conclusions: Congenitally missing teeth were observed in about 4–5% of the studied population with a female predilection. The lower second premolar was the most commonly absent tooth, followed by the maxillary lateral incisors. An Angle’s Class II malocclusion was present in the majority of orthodontic patients with hypodontia, mostly in the mandible.
Polytrauma Caused by a Bear Attacking a Human with a Benign Outcome
Authors: Mellin Ruslan , Velichko Ellina , Maltseva Larisa , Dydykin Sergey , Vasil’ev Yuriy
Published: February 2024

Injuries to humans caused by wild animals, particularly bears, are rarely mentioned in the literature. Such injuries are frequent in Siberia, which is a territory surrounded by dense forests inhabited by brown bears. In the last 4 months alone (September–December 2023), four bear attacks on humans were registered in Khakassia, Russia. This article presents a clinical case of rehabilitating a patient after a bear attack, who suffered multiple fragmentary fractures of the facial skeleton with displaced bone fragments, subcutaneous emphysema of the soft tissues of the face, damage to the parietal and right occipital regions and paranasal sinus hemorrhage on the left side. The nature of the injuries was enhanced by trauma to the upper extremity caused by the patient defending himself against the animal. In addition to the damage to his face, the bear tried to open his cranium, as evidenced by four furrows caused by its canines, including two each on the frontal and occipital bones of the skull. The patient’s complex treatment included both maxillofacial and reconstructive surgeries, and outpatient treatment involved the formation of normotrophic scars using a neodymium laser and injections of a heterogeneous composition consisting of microparticles of “crosslinked” collagen of animal origin placed in a gel identical to the natural extracellular matrix.