bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitigación del déficit hídrico por aplicación de calcio en papa amarilla diploide (Solanum tuberosum L. grupo Phureja)
Authors: Cárdenas Pira Wendy Tatiana , Moreno Fonseca Liz Patricia , Rodríguez Luis Ernesto
Published: February 2024

La variabilidad climática favorece la ocurrencia de sequías que disminuyen el rendimiento en cultivos de papa, por lo cual es necesario desarrollar estrategias para mitigar los efectos del déficit hídrico. El objetivo de esta investigación fue evaluar el efecto de la aplicación de calcio (Ca) en parámetros fisiológicos y rendimiento de Solanum tuberosum L. grupo Phureja cultivar criolla Colombia bajo déficit hídrico. Tubérculos-semilla fueron sembrados en bolsas y mantenidos a capacidad de campo hasta el inicio de tuberización. Entre los 44 y 54 días después de siembra (dds), se realizaron aplicaciones edáficas (E) de: CaCl2 de 3 y 4.5 g planta-1; Ca(NO3)2 de 4.5 y 7.0 g planta-1 y Ca(NO3)2 + B de 6.0 y 9.0 g planta-1. También se realizó aplicación foliar de: CaCl2 de 10 y 20 mM planta-1; y Ca(NO3)2 de 10 y 20 mM planta-1. A los 55 dds las plantas fueron sometidas a riego continuo (BR) y déficit hídrico por suspensión de riego durante 7 días (DH). El DH redujo el contenido relativo de agua en un 22.1 %, el potencial hídrico foliar y el rendimiento en un 26.7 %, y aumentó la pérdida de electrolitos (97.3 %). Las plantas con CaCl2–20-F, CaCl2–4.5-E y Ca(NO3)2–7-E, presentaron menor pérdida de electrolitos (<21.2 %), mayor contenido relativo de agua (>12.6 %) y rendimiento (>15.6 %), respecto a las plantas sin Ca. Esto indica una mitigación del estrés hídrico a nivel de la estabilidad de la membrana y el estado hídrico en aplicaciones edáficas y foliares de Ca, lo cual probablemente se relacione con sus funciones como molécula señalizadora.
Efecto de la temperatura ambiente en la producción de leche de vacas Holstein en el departamento de Nariño, Colombia
Authors: Trejo Casanova Carmen del Rocío , Sánchez Guerrero Hugo , Timarán Rivera Edmundo Andrés , Burgos Arcos Álvaro Javier , Solarte Portilla Carlos Eugenio , Rivera Palacio Juan Camilo , Molina Benavides Raúl Andrés
Published: February 2024

La temperatura ambiental es un factor influyente en el bienestar animal y su impacto se refleja principalmente en la salud y productividad. El objetivo de esta investigación fue evaluar el efecto de la temperatura ambiental mínima sobre producción y calidad de leche en vacas Holstein, realizando control lechero en fincas del departamento de Nariño (Pasto, Pupiales y Guachucal). Se registró el volumen de leche producido por vaca en el día del control y se obtuvieron muestras de leche para su posterior análisis (grasa, proteína, sólidos totales y células somáticas). La información de temperatura ambienta se obtuvo de las estaciones meteorológicas del Instituto de Hidrología, Meteorología y Estudios Ambientales (Ideam) cercanos a los hatos. Se empleó un modelo lineal mixto que contempla el efecto fijo de la temperatura ambiental mínima, número de parto, días de lactancia y, como efecto aleatorio, el distrito lechero. Los resultados muestran que la composición de la leche y el conteo de células somáticas no fueron afectados por las temperaturas ambientales mínimas, pero sí hay un efecto significativo sobre la producción de vacas con seis o más partos, pues se dio una disminución de hasta 2.6 l. Esta reducción en la producción de leche posiblemente se atribuye a la disminución del consumo y a la utilización de la energía para la producción de calor y mantenimiento de la temperatura corporal como mecanismo de supervivencia. Para posteriores investigaciones se sugiere incluir variables fisiológicas del animal (temperatura corporal, consumo de materia seca, niveles de cortisol) y del ambiente (precipitación, viento, humedad relativa, radiación solar).
Contribution of community-based companies to sovereignty and food security in the Central Highlands of Ecuador
Authors: Franco-Crespo Christian , Tibán Luis
Published: February 2024

Popular and solidarity economy (PSE) organizations number around 5,280 throughout Ecuador, with the participation of 153,390 registered members. However, only 1.02% of these companies are community-based and operate in the food production and processing sector. This research aimed to determine the contribution of community-based companies (CBCs) to food sovereignty in the province of Tungurahua, using a mixed methodology including the use of interviews and surveys. The qualitative analysis integrated data on the features of this sector to observe their relationship with social, environmental, and economic dimensions using the RQDA package in R Studio. The results show that CBCs contribute to food sovereignty by adding value to locally produced products relevant to local traditions and culture. The social and environmental contributions of CBCs play a part in rural development. Thus, the promotion of CBCs in food production represents a mechanism through which to strengthen food sovereignty and preserve food production relevant to the local culture in these areas.
Percepción de mejores servicios públicos desde el impulso a la industria turística, Noreste de México
Authors: LUYANDO JOSE RAUL
Published: February 2024

La presente investigación se enfoca en la percepción que tienen los pobladores del Municipio de Ciudad Madero en el Noreste de México, con respecto de si consideran que el impulso que se ha dado a la industria turística en los últimos años en la región ha servido para generar una mayor inversión en algunos de los servicios que oferta o regula generalmente el sector público y que pueden impulsar la calidad de vida de sus habitantes. Para hacerlo, se utiliza una muestra de 384 individuos que habitan el lugar y los resultados se obtienen utilizando la técnica de análisis factorial confirmatorio. Como un resultado general se puede señalar que existe la percepción en la muestra de que el impulso a esta actividad ha afectado de forma positiva una serie de servicios que pueden mejorar sus condiciones de vida.
Recolha de circunstâncias: o diário como álbum
Authors: Pimentel Pinto Júlio
Published: February 2024

O artigo analisa a caracterização do gênero diarístico como álbum, a partir da diferenciação mallarmaica entre Livro e Álbum, da abordagem de Roland Barthes sobre o tema e do caso específico representado pela trilogia Os diários de Emilio Renzi, de Ricardo Piglia. Para tanto, analisa questões como: a morte e o retorno da figura autoral, as escritas de si, a pluralização de enunciadores, a forma de diário e sua dimensão fragmentária, a relação entre o registro no presente e a evocação do passado. Atesta-se a possibilidade de identificar os diários de Piglia/Renzi como um álbum, enfatizando sua composição plural e as estratégias empregadas na sua montagem.
Nuevos “habitus” de consumo entre los jóvenes asalariados rurales
Authors: Martínez Valle Luciano , Martínez Godoy Diego
Published: February 2024

En este artículo se analiza el proceso de integración a nuevos patrones de consumo de los jóvenes asalariados rurales en un territorio de la sierra ecuatoriana, donde predominan los agronegocios de flores y brócoli.  Se busca mostrar las condiciones estructurales que permiten a los jóvenes asalariados adoptar nuevos habitus  más cercanos al consumo de mercancías globales, proceso estrechamente relacionado con su posición ocupada en el campo social. En una primera parte, analizamos la relación entre consumo y “habitus” en el medio rural desde distintas perspectivas teóricas sociológicas. En una segunda parte, a partir de un estudio de caso, se abordan las tendencias del consumo entre los jóvenes rurales. En una tercera parte se reflexiona sobre la importancia de nuevas pautas de consumo entre los jóvenes asalariados a través del acceso a dos tipos de mercancías globales (motocicleta y celular) que se han convertido en nuevos objetos simbólicos y de prestigio.
Estudo transversal descritivo das notificações dos acidentes por queimaduras em pessoas idosas residentes da cidade de São Paulo
Authors: Leci Rodrigues Cintia , Manso Maria Elisa Gonzalez
Published: February 2024

Introdução: as lesões por queimadura entre as pessoas idosas são acidentes que, em grande parte, ocorrem no domicílio sendo, em sua maioria, preveníveis. Há necessidade de estudos nesta temática, já que esta interfere na necessidade de cuidados médico-hospitalares e no aumento da permanência durante a internação, devido ao pior prognóstico e resultante aumento da mortalidade neste segmento etário.
Objetivo: analisar as notificações dos acidentes por queimadura na cidade de São Paulo durante o ano de 2022.
Métodos: trata-se de um estudo transversal, descritivo e retrospectivo, realizado mediante levantamento de dados obtidos através do Sistema Informação para a Vigilância de Violência e Acidentes da Secretaria Municipal de Saúde da cidade de São Paulo, no qual são registradas as notificações por queimadura por meio da ficha de notificação de casos.
Resultados: durante o ano de 2022 foram notificados 201 casos de acidentes por queimaduras na população acima de 60 anos de idade residente na cidade de São Paulo. Estes acidentes tiveram maior frequência em mulheres, na faixa etária inferior a 70 anos de idade e no ambiente doméstico.
Conclusão: o estudo contribui para o conhecimento acerca de uma temática pouco estudada e sugere que tais aspectos precisam ser mais pesquisados e considerados nas ações de políticas públicas voltadas à saúde da pessoa idosa, com o intuito de reduzir a ocorrência dos acidentes em geral e, em especial, por queimadura, visando minimizar os impactos que estes provocam na qualidade de vida.
RADEN SAWUNGGALING TOMB'S HISTORICAL NARRATIVES AND ITS PROSPECTIVE IMPACT ON SURABAYA'S TOURISM
Authors: Rafsanjani Afida Rizma Liana , Nafisah Ainatun
Published: February 2024

ABSTRACTThis research is field research, namely by carrying out field activities that are used to obtain various data from the information needed. The aim of this research is to determine the history, existence, layout and potential of the Raden Sawunggaling Tomb as a religious tourism object. The results of this study are the existence of Raden Sawunggaling's grave on Jalan Lidah Wetan gang III, Lidah Wetan Village, Lakarsantri District, Surabaya City. The tomb was found around 1908, some say it was 1901. The local community responded well to the existence of Raden Sawunggaling's tomb, even though there had been suspicions about Raden Sawunggaling. Raden Sawunggaling's grave was officially made a Cultural Heritage site in 2013. The Raden Sawunggaling grave area is in the middle of a residential area with a building area of around 44 m2. The potential for the tomb to become a religious tourism object is very good, because Lidah Wetan sub-district is a big economic wheel for West Surabaya, such as the Pakuwon shopping center and also UNESA. However, Raden Sawunggaling's tomb also has several challenges to become an even bigger tourist attraction.Keywords: Joko Berek, Raden Sawunggaling, Surabaya, Tourism. ABSTRAKPenelitian ini merupakan penelitian lapangan, yakni dengan melakukan kegiatan lapangan yang digunakan untuk mendapat berbagai data dari informasi yang dibutuhkan. Tujuan penelitian ini ialah untuk mengetahui sejarah, eksistensi, tata letak, dan potensi Makam Raden Sawunggaling sebagai Objek Wisata Religi. Hasil dari penelitian ini adalah keberadaan makam Raden Sawunggaling ada di jalan Lidah Wetan gang III, Kelurahan Lidah Wetan, Kecamatan Lakarsantri, Kota Surabaya. Makam tersebut ditemukan sekitar tahun 1908-an, ada juga yang menyebut tahun 1901. Masyarakat sekitar merespon baik atas keberadaan makam Raden Sawunggaling, walaupun sempat terjadi kecurigaan terhadap Raden Sawunggaling. Makam Raden Sawunggaling resmi dijadikan sebagai situs Cagar Budaya pada tahun 2013. Kawasan makam Raden Sawunggaling berada di tengah-tengah pemukiman penduduk dengan luas bangunan sekitar 44 m2. Potensi makam bila dijadikan objek wisata religi sangat bagus, karena di kecamatan Lidah Wetan sebagai roda ekonomi Surabaya Barat yang besar seperti adanya pusat perbelanjaan Pakuwon dan juga UNESA. Namun, makam Raden Sawunggaling juga memiliki beberapa tantangan untuk dijadikan daya tarik wisata yang lebih besar lagi.Kata kunci: Joko Berek, Raden Sawunggaling, Surabaya, Wisata.
What should a dictionary contain to be useful for translators?
Authors: Buendía-Castro Míriam
Published: February 2024

Translators are one of the main groups of dictionary users. The design, revision, and compilation of any kind of lexicographic or terminographic resource for translators should thus be based on their specific needs. To this end, the research examined the importance attributed by 221 BA and MA trainee translators to the various types of information in a dictionary entry, with a view to determining which are most useful for translation. Also identified were the differences between an ideal entry for translators and one for the general public. The results showed that the students’ answers varied depending on their year of study, as well as whether they were on the BA or MA program. The data collection tool was a self-reported questionnaire. The dictionary information most valued by trainee translators was usage contexts, definitions, phraseology, and translation equivalents. Furthermore, students at higher academic levels attributed more importance to usage contexts and phraseology. This study highlights the need to include phraseological and contextual information in the entries of translation-oriented dictionaries.
IMPLANT SURVIVAL IN RENAL-TRANSPLANTED PATIENTS: A PROSPECTIVE LONG-TERM STUDY.
Authors: Berton Federico , Zotti Matteo , Rapani Antonio , Costantinides Fulvia , Castronovo Gaetano , Di Lenarda Roberto , Stacchi Claudio
Published: February 2024

The present study aims to assess the feasibility of implant rehabilitation in kidney-transplanted patients. Patients with kidney transplantation included in periodontal supportive care and at least one year of dialysis with mono- or partial edentulism were eligible for this study. Histomorphometric evaluation of the harvested bone was matched with radiological bone assessment. Implant stability was also monitored with RFA and ITV. Fixed cemented prostheses have been delivered after conventional loading protocol. Supportive periodontal therapy has been administered. Eleven patients (9 males and 2 females) were included. The mean age was 58.1 ± 9.9 years. A total of 17 implants were inserted and analyzed. Mean ITV was 39.3 ± 23.8 Ncm. The mean primary stability (ISQ) at T0 was 71.7 ± 10.5, whereas the mean secondary stability at T1 was 73.0 ± 7.3. The minimum follow-up was 62 months, with a maximum of 84 months (7 years) reached by four patients. Fourteen out of 15 implants were in function at a 5-year follow-up (survival rate 93.3%). Two implants showed peri-implantitis. Seventeen bone samples were collected (13 in the mandible and 4 in the maxilla). The mean percentage of marrow spaces and lamellar bone was 41.6% and 58.4%, respectively. Class 3, according to Misch classification, was found as the mean value of radiological bone density. It can be concluded that implant-supported rehabilitation in kidney-transplanted patients is possible. Adequate periodontal maintenance allows implant rehabilitation in kidney transplanted patients with long-term sufficient survival rates.
Tathwîr Al-Mawâd Al-Ta’lîmiyah li Mahârah al-Istimâ’ al-Mustamidah min as-Saqâfah al-Mahalliyah ladâ Tholibât al-Jâmi’ah
Authors: Rosyad Rosyad , Baihaqi M. , Nuha Muhammad Afthon Ulin
Published: February 2024

Purpose- This study aims to develop teaching materials for listening skills based on local content, determine the level of validity of teaching materials, and measure the level of effectiveness of the developed teaching materials.
 Design/Methodology/Approach- This study uses research and development methods based on the Borg and Gall model with special treatment from researchers. Sources of data from students, expert councils, and policy makers in Islamic boarding schools were obtained through observation, interviews, and tests. The data will then be analyzed qualitatively and quantitatively simultaneously with interactive analysis and t-test. The subjects of this study were all female students of the intensive Arabic language program who were determined through a purposive technique, with a total of 25 samples.
 Findings- Among the results of this study were the results of the pre-test reaching 1882 with a rate of 75.28, while in the post-test it reached 2045 with a rate of 82.16. The effectiveness of the developed material is seen at a score of 12.69 which is greater than the table score at the 2.80 level. Thus teaching materials developed based on local content are effective in improving the listening skills of female students of the intensive Arabic language program.
Research Limitation/Implications- The formal finding of this research is that teaching materials that are arranged thematically based on local content can improve Arabic listening skills for students.
Manajemen Kepala Madrasah Dalam Mencapai Visi, Misi, dan Sasaran Pada MIN 31 Kabupaten Aceh Utara
Authors: Syukri Syukri , Hasibuan Hasan , Siregar Zulfikar Ali Buto
Published: February 2024

This study aims to determine planning, implementation, evaluation and supporting and inhibiting factors for the management of the Madrasah Principal in achieving the Vision, Mission and Targets at MIN 31 North Aceh District of North Aceh. Sources of data in this study included the Head of Madrasah, Deputy Head of Curriculum, and teachers of North Aceh MIN 31 North Aceh. This study uses a qualitative method with a descriptive approach. Data collection techniques in this study include observation, interviews and documentation. The results of the study show that first, planning for the management of the madrasah head in achieving the vision, mission and goals of North Aceh MIN 31 is carried out Formulation of character education programs that are integrated with the madrasah curriculum, holding activities to commemorate Islamic holidays, social services, holding congregational midday prayers, socialization of academic regulations, and monthly, mid-semester and annual coaching, and giving rewards and sanctions both routinely and incidentally. Second, the management of Madrasah heads in achieving the vision, mission and goals of MIN 31 North Aceh is carried out at MIN 31 North Aceh is carried out by determining the types of extracurricular activities that suit the talents and interests of students and determining character values ​​in each type of extracurricular activities by the coach extracurricular, Third, Evaluation of the management of the Madrasah head is carried out by determining the character values ​​to be instilled, the formation of the madrasa culture, and the supporting and inhibiting factors of the Madrasah Head in achieving the vision, mission and goals at MIN 31 North Aceh. The four supporting factors are: the principal's leadership factor, the educational qualification factor of all educational staff and education staff, the support factor from the community and school members including committee members and parents. While the inhibiting factors include the indiscipline of teaching and educational staff in carrying out programs, the lack of public understanding related to achieving the madrasah vision, mission and goals. and the lack of quality capabilities of all stakeholders in improving the quality of madrasas
Pemanfaatan Sistem Informasi Manajemen pada Aplikasi Pangkalan Data Ujian Madrasah (PDUM) dalam Meningkatkan Efektivitas Ujian Siswa Tingkat Akhir Madrasah Aliyah Negeri di Kementerian Agama Kabupaten Lamongan
Authors: Achmad Deydik Kurnia  , Fatihatun Nashiroh  , Sulanam
Published: February 2024

Penelitian ini bertujuan untuk memperoleh informasi mengenai pemanfaatan sistem informasi manajemen pada aplikasi pangkalan data ujian madrasah (PDUM) dalam meningkatkan efektivitas ujian siswa tingkat akhir madrasah aliyah negeri di kementerian agama kabupaten lamongan yang ditinjau dari aspek: input, proses, dan output. Metode yang digunakan adalah metode deskripsi, melalui observasi dan studi pustaka, yaitu dengan penelitian di Kementerian Agama Lamongan dan MAN 1 Lamongan selain itu juga mengumpulkan materi, data dan informasi dari buku, jurnal dan artikel yang berkaitan dengan masalah yang dibahas. Hasil penelitian yang didapat adalah pemanfaatan sistem informasi manajemen sudah diterapkan dengan baik. MAN 1 Lamongan menerapkan strategi khusus yaitu memanfaatkan teknologi informasi berbasis Sistem Informasi Manajemen, pemanfaatan aplikasi PDUM yang berfungsi sebagai perhitungan kebutuhan blanko ijazah, Selain itu PDUM juga digunakan untuk cetak kartu ujian, berita acara, dan daftar hadir yang dibuat menggunakan sistem. Maka dapat diketahui bahwa keefektifan PDUM terhadap ujian akhir siswa di Madrasah Aliyah telah diterapkan dengan baik.
ISLAMIC GENETICS IN INDONESIA: Tracking the Hindu-Islamic Dialogue Concerning the Growth and Development of Javanese Islam
Authors: Basyir Kunawi , Riyadi Abdul Kadir , Warsito Loekisno Choiril
Published: February 2024

This article examines the genetics of Islam in Indonesia, namely Javanese Islam. Through the lens of cross-cultural theology, this research aims to observe the dialogue and interaction between Hindu tradition and culture and Islamic religious practices and behaviors. Employing anthropological and sociological approach with an intensive library research method, this research found out that the style and behavior of Javanese Islam have resulted from the dialogue, interaction, negotiation, and synergy with Hindu (local) culture and traditions. The result also shows that Islam can harmonize and synergize its teachings with the local culture while maintaining its values or doctrines. Hence, Javanese Islam is better called Local Islam or Islam Nusantara rather than Syncretic Islam.
The Western Perspective on Islam: Reading the Legacy of Snouck Hurgronje on Islamic Studies
Authors: As'ad Muhammad , Zainuddin Muhammad , Hady M. Samsul
Published: February 2024

This article discusses the Western perspective on Islamic studies, particularly through the academic legacy of Snouck Hurgronje, a Dutch scholar known for his research on Muslim culture in Aceh and his influence on the Dutch colonial strategy in the Dutch East Indies. The author aims to answer two questions: what were the methods and characteristics of Islamic studies used by Snouck Hurgronje, and how do they differ from contemporary Islamic studies? Utilizing library research, it examines scholarly literature on Hurgronje, comparing his methods to current Islamic studies practices in Western universities and Indonesia. The article sheds light on Hurgronje’s role in introducing anthropology and ethnography to Islamic studies, noting his significant contributions to the development of ethnographic methods in this area. It suggests that his approach has been influential in shaping the study of Islam and continues to influence contemporary research methodologies in the field. However, the article also notes that 21st-century Islamic studies are fundamentally different from Hurgronje’s era, with a greater focus on insider researchers and a lack of imperialistic interests. These scholars, particularly those in Indonesian universities under the Ministry of Religious Affairs, have a significant influence on the field and continue the legacy of Hurgronje by using philological, anthropological, and ethnographic approaches to Islamic studies.
Antibodies to cyclic citrullinated peptide and angiopoietin-like protein type 4 as markers of immune inflammation and osteoporotic processes in rheumatoid arthritis patients
Authors: Osmanova G. Ya. , Aleksandrov V. A. , Aleksandrov A. V. , Shilova L. N. , Cherkesova E. G. , Aleksandrova N. V. , Zborovskaya I. A.
Published: February 2024

Low-energy fractures in rheumatoid arthritis (RA) are more common in patients with high activity and long duration of disease, and with high titers of anti-citrullinated antibodies (ACPA). Increased expression of angiopoietin-like protein type 4 (ANGPTL4) in bone tissue has also been noted in inflammatory arthritis. The purpose of the present study was to analyze the effect of ACPA and ANGPTL4 on systemic bone mineral density in RA patients. Antibodies to ACPA and ANGPTL4 content were detected in blood serum of 96 RA patients (women, 91.7%) by enzyme immunoassay. Mineral density of the lumbar vertebrae (BMDL1-L4), hip neck, and entire femur (BMDtotal) was measured by dual-energy X-ray absorptiometry (DXA). In study group, the ACPA and ANGPTL4 tests were positive in 61.5% and 41.7% of patients, respectively. Negative correlations were shown between ACPA and BMDtotal, and of ANGPTL4 with BMDL1-L4 (p < 0.05). Separation of the patients into groups with low (n = 34) and high (n = 62) DAS28 activity demonstrated a significant increase in ACPA with increasing RA activity (p = 0.042). ACPA and ANGPTL4 scores were also significantly higher in the group of 45 RA patients with osteoporosis (OP) compared to the RA group without OP (n = 51) showing significant difference at p = 0.002 and p = 0.028, respectively. Patients’ age, body mass index (BMI), duration and activity of the disease had no significant effect on ACPA in the general group of RA patients. However, the correlation between ACPA and DAS28 proved to be significant in the group of RA patients with OP (b = 0.31, p = 0.039). Among all presented variables, the disease duration was the only significant factor for ANGPTL4 in the total group of RA patients (b = 0.31, p = 0.039). In the regression model, BMDtotal showed similar correlations with patients’ age (b = -0.28), BMI (b = 0.25), and ACPA level (b = -0.26). A search for association between BMDL1-L4 and various RA characteristics demonstrated a strong correlation with ANGPTL4 only (b = -0.74; R2 = 0.57). The revealed correlation between ANGPTL4 and decreased BMD specifically in the spongy layer of bone allows us to identify the RA patients with high ANGPTL4 levels as a risk group specifically for spinal fractures thus considering ANGPTL4 as a potential target for treatment of osteoporotic disorders.
Comparative characteristics of the cellular immune response to SARS-CoV-2 during infection and post-vaccination
Authors: Klyueva S. N. , Bugorkova S. A. , Kravtsov A. L. , Kashtanova T. N. , Kozhevnikov V. A.
Published: February 2024

An important area of research concerns monitoring of immune response features in patients with SARS-CoV-2 infection as well as their analysis, as compared with characteristics of vaccine-mediated protection, in order to specify the determinants of cellular immune response. The aim of our work was to compare the state of cellular immune response in patients who underwent COVID-19, and in persons vaccinated with a peptide vaccine preparation. The study involved volunteers who suffered with COVID-19 of varying severity (n = 30), as well as persons who completed the full course of vaccination with the peptide vaccine (n = 27). For comparison, we took blood specimens from the volunteers before vaccination. Immunophenotyping of leukocytes was performed by the Lyse/No-Wash procedure (BD Bioscience, USA), and Cyto-Stat monoclonal antibodies (CD45-FITC, CD4-PE, CD8-ECD, CD3-PC5), CD45RA-PC7, CD45RO-PE (Beckman Coulter, USA), and analyzed with a DakoCytomation flow cytometer (Denmark). Determination of intracellular IFNγ (CD4+IFNγ+) was performed with the standard technique. Cytokine production was determined using reagent kits for detection of IFNγ, TNFα, IL-4, IL-8, IL-10 (Vector-Best JSC, Russia) with automatic enzyme immunoassay analyzer LAZURIT (Dynex Technologies, USA). As based on the results obtained, we have shown that cellular immunity was developed after vaccination and infection with COVID-19. However, the most pronounced immune response was recorded in the COVID-19 reconvalescents, i.e., more than 60% of these patients showed an increased number of CD4+T-memory helper cells (8.7 (0.5-12.1) % versus 0.3 (0.1-0.5) % in the comparison group, p < 0.05) as well as proportion of CD4+IFNγ+T lymphocytes (4.2 (1.8-4.3) % versus 0.4 (0-0.8) % in the comparison group, p < 0.05). Moreover, we revealed an increased functional reserve of cells in terms of TNFα, IL-8, IL-10 production. One month after vaccination of volunteers with the peptide-based preparation, the total pool of memory T lymphocytes was apparently dominated by CD8+T memory cells (CD45+CD8+CD45RA-CD45RO+). A significant increase was found in the average levels of CD4+IFNγ+ activated cells (8.2-fold), as well as in values of ConA-induced IL-4 production (3.3 (1.1-4.5) pg/mL, and 2.8 (1.7-3.9) pg/mL, respectively versus 1.3 (0.1-2.4) pg/mL in the control group, p < 0.05). The data obtained are in accordance with information available in the literature concerning development of cellular immune responses to SARS-CoV-2, which results from a past illness, or measures for the specific prevention of COVID-19. Further search for cellular correlates of protection against a new coronavirus infection will allow us to revise the current vaccination strategy and develop an optimal approach to COVID-19 prevention.
Cytokine profile of oral fluid in patients with food allergy associated with atopic dermatitis and psoriasis
Authors: Barilo A. A. , Smirnova S. V. , Peretyatko O. V.
Published: February 2024

The world is experiencing a rapid increase in the prevalence of allergic and autoimmune diseases. It is known that allergic inflammation is most often systemic, involving various organs and systems in the pathological process, such as the skin, respiratory and gastrointestinal tract with the development of dermatorespiratory, dermato-intestinal and other manifestations. The study of the features of the cytokine profile in oral fluid (saliva) deserves special attention, since these characteristics reflect not only local, but also systemic disorders. Of particular relevance is the study of local cytokine regulation of intercellular interactions in food allergies. Our objective was to study the concentration of IL-4, IL-10, IFNγ, secretory IgA in salivary fluid, the concentrations of total immunoglobulin E and eosinophilic cationic protein in blood serum of the patients with atopic dermatitis and psoriasis with concomitant food allergies.The study included patients with atopic dermatitis (AD, group 1, n = 20), psoriasis with concomitant food allergy (PS, group 2, n = 27), psoriasis without concomitant allergies (PS, comparison group 3, n = 23). Quantitative assessment of the cytokine concentrations (IL-4, IL-10, IFNγ, sIgA) in salivary fluid was carried out by enzyme-linked immunosorbent assay. Concentrations of total immunoglobulin E and eosinophilic cationic protein in blood serum were determined by indirect immunofluorescence. The obtained results were processed using the Statistica 8.0 applied software.In groups of patients with atopic dermatitis (Group 1) and psoriasis with concomitant food allergy (Group 2), we have noted a statistically significant increase of salivary IL-4 and IL-10, as well as of total immunoglobulin E concentrations in blood serum as compared with a group of patients with psoriasis without concomitant allergies (group 3), and with control group. When studying concentrations of IFNγ in saliva, no statistically significant intergroup differences were found. The concentration of sIgA in saliva was significantly higher in the groups of patients with atopic dermatitis and psoriasis accompanied by food allergies in comparison with control group and the group of psoriatic patients without food allergies (group 3).The cytokine profile of saliva is characterized by unidirectional changes in food allergy. Skin seems to be the shock organ in this condition, regardless of nosological form of the disease (atopic dermatitis or psoriasis). Salivary fluid is an easily accessible material when assessing the state of mucosal immunity in food allergies.
Increased expression of tyrosine protein phosphatase (CD45) on the surface of human blood granulocytes under the influence of the plague microbe vaccine strain Yersinia pestis EV NIIEG ex vivo and in vivo
Authors: Kravtsov A. L. , Klyueva S. N. , Kozhevnikov V. A. , Bugorkova S. A.
Published: February 2024

Tyrosine protein phosphatase (common leukocyte antigen CD45) regulates FcᵧR-mediated cell signaling and secretory function of neutrophilic granulocytes (NG) when interacting with antigen-antibody immune complexes. The aim of the work is to study changes in the expression of CD45 on the surface of human granulocytes in ex vivo modeling of bacteremia by live cells of the plague microbe vaccine strain Yersinia pestis EV NIIEG and to evaluate the priming effect of the live plague vaccine (LPV) in vivo in terms of this parameter. The expression density of CD45 on NG was determined by flow cytometry in conventional units of fluorescence intensity (MFI) after staining the cells with the CD45-FITC labeled mouse antibody reagent (Backman Coulter, USA) during immunophenotyping of blood leukocytes according to the Lyse/No Wash protocol. In donors not previously vaccinated against plague (group 1), the value of the indicator was assessed before and 30 min, 2 h, 6 h after the addition of Y pestis EV cells to whole blood at a dose of 108 mc/ml, as well as 1 month and 6 months after the primary anti-plague vaccination. In individuals who had previously been repeatedly vaccinated with LPV in the territory of the natural plague focus (group 2), CD45 expression on blood granulocytes was determined one year after the last annual vaccination, and then 1 month and 6 months after revaccination. Getting into human blood, living cells of the vaccine strain Y pestis EV of the plague microbe induced a change in the NC phenotype already after 30 minutes, associated with a 3.5-fold increase in the surface expression of CD45, which remained at an elevated level for 6 hours. The studied indicator depended ex vivo on the degree of resistance of plague microbes to phagocytosis and killing of NG. Plague vaccination had a similar stimulating effect on human peripheral blood NG in vivo. Under the influence of HPV, CD45 expression increased on blood NG in groups 1 and 2 one month after vaccination, and the changes persisted in volunteers for 6 months. The experimental data obtained in the work may reflect the result of NG priming with lipopolysaccharide and other Y. pestis antigens. The registered functional activation of NG by expression of tyrosine protein phosphatase probably indicates the formation of “immune memory” at the level of innate immunity cells under the influence of LPV, the functioning of which explains the development of a faster and more intense antigen-specific immune response to repeated introduction of the plague vaccine into the body.
Phenotypic features of dendritic cells when using different modes of their stimulated maturation
Authors: Fadeyev F. A. , Aleksandrova A. D. , Mogilenskikh A. S.
Published: February 2024

Monocyte-derived dendritic cells (DCs) can be used for cell immunotherapy of cancer. In most cases, mature DCs, loaded with tumor-associated antigens, are used for immune therapy. The functionality of DCs for immunotherapy substantially depends on their immunophenotype and secretory profile, which are established after DCs maturation. The purpose of this research was to explore the phenotype of DCs after using various approaches for stimulation of their maturation.

Maturation of DCs was stimulated by pro-inflammatory cytokines and their mixtures, or by ligands to the TLRs of DCs. DCs were stimulated by the following means: TNF; poly I:C; LPS; cytokine cocktail (TNF + IL-1 + IL-6 + PGE2); the cocktail mixed with poly I:C; and melanoma cells lysate. Forty-eight hours after stimulation, the expression of DCs’ receptors involved into their interaction with T cells, was evaluated by flow cytometry. Moreover, the secretion of IL-12 (activator of T cell response) and IL-10 (inhibitor of T cell response) was estimated by ELISA technique.

We have shown that, following stimulation with cytokine cocktail, the DCs exhibit highest expression of receptors, which are necessary for interaction with T cells and for activation of T cell mediated immune response, i.e., antigen-presenting receptors (HLA-DR), co-stimulatory receptors (CD83, CD40, CD86), and receptors controlling the migration of DCs to lymph nodes (CCR7). Moreover, the cocktail-stimulated DCs intensively secrete both IL-12 and IL-10. The stimulatory effect of TNF and poly I:C proved to be moderate: the expression of most receptors was significantly lower than after using the cocktail; no significant differences from control (in absence of induced maturation) in IL-12 secretion were detected. LPS and melanoma cell lysate did not affect both expression of receptors and secretory profile of DCs. Addition of poly I:C to the cytokine cocktail did not affect the receptor expression, but significantly increased the secretion of both proinflammatory IL-12 and anti-inflammatory IL-10.

The results of experiments demonstrate that the mixture of cytokine cocktail and poly I:C seems to be the most effective tool for stimulation of DCs maturation. However, further experiments are required to compare the functionality of DCs when using different tools for induced DC maturation.
Rewolucja seksualna jako droga do ponowoczesności – analiza twórczości Wilhelma Reicha w świetle współczesnej dekonstrukcji kultury patriarchalnej
Authors:   Uniwersytet Jagielloński w Krakowie, Lipowicz Markus
Published: February 2024

The aim of the present article is a presentation of Wilhelm Reich’s works as one of the most important intellectual foundations of postmodernity. Particularly the propagated by Reich sexual revolution constitutes the kernel of contemporary cultural changes in the West. An important point of reference for Reich were the revelations of Bronisław Malinowski on the sexual life of savages. These convinced Reich that there is no necessary conflict between the satisfaction of sensual desires and cultural life, as Sigmund Freud proclaimed. In order to overcome the dualism between nature and culture in the western culture Reich deconstructed the ecclesiastic image of Jesus Christ. Instead of a savior Reich suggested that one should see in Jesus a man who proclaimed reconciliation between the forces of nature and the social life. In this way Reich tried to convince that in place of ascetic moral values the ones that promote sexual liberty and individual satisfaction should be placed. I will try to demonstrate that the ideas of Reich constitute a fundamental intellectual source of the contemporary western culture.
Perception of life rhythm - aesthetic philosophy in music education
Authors: Xu Hanmei
Published: February 2024

Abstract: As an aesthetic education, music education not only needs to convey the beauty of art, but also is an educational way to convey the beauty of life. Therefore, based on life aesthetics and life connotation of education, the research discusses the life orientation of multi-level music education from multiple perspectives. The research analyzes the life aesthetics of music from three aspects: the limited and the infinite, the cultural existence of the humanistic spirit and the isomorphism of the life state. The discussion on the connotation of educational life starts from three aspects: survival, living and life. On this basis, the research integrates the above two theories and discusses the subjective-communication, cognition and cognitive emotion-experience in music education. Through the basic elaboration of the life vision of music education, it can be found that music education is to help students to pursue a life full of art by helping them develop harmoniously.
Marxist foundation and historical development: interpretation and practice in China’s new era
Authors: Chen Meixu
Published: February 2024

Abstract: Professor Chen Xianda’s required course of Marxist Philosophy in the new era interprets the theory of socialism with Chinese characteristics in the new era theoretically, and tells people the Marxist philosophical basis of the theory of socialism with Chinese characteristics in the new era in a simple way. He wants to teach people to comprehensively and deeply understand the theory of socialism with new era characteristics on the basis of reading the original, learning it and understanding the principles, so as to better lead the practice. Among them, “learning to understand” requires leading cadres to accurately understand the logic system and theoretical origin of the socialist theory in the new era, and to enter the mind into the heart into the action. “Getting through” requires leading cadres to accurately grasp the theoretical logic, the historical one and practical logic of the thought in the new era, and to learn to examine the problems faced in the process of socialist construction from the perspective of the whole and development. “Being realistic” is required that the theory of leading cadres should be combined with practice, and the socialist thought, with Chinese characteristics in the new era, should be carried out and implemented in the specific work of this region and this department.
Chinese animation and its evolution and cultural background
Authors: Man Shengchong
Published: February 2024

Abstract: Philosophy is a system that analyzes the most basic beliefs, concepts and calmness of temper and judgement befitting a philosopher. Chinese animated features have made great inroads in worldwide markets in recent years, despite the fact that China’s animation sector is just a teenager compared to its decades-old counterpart in Japan’s philosophy. While some animation studios are creating content specifically for international audiences, the Chinese animation industry is primarily concerned with the domestic market because it believes that high-quality Chinese animation has the potential to travel. Artists, philosophers and those in the creative industries, directly tied to this, have an incentive and challenge to discover those stories that can succeed in Chinese and international markets. Therefore, this article analyzes and contrasts the characteristics of Chinese film animation philosophies. The social and cultural context, market economic development and technological advancement that have shaped the philosophical design of Chinese animation are examined. The philosophical approaches that are assessed in this study are Donghua and Manhua, and Daoist philosophy that exemplified from Zhuangzi.
Dissociation and re-imagination: the publicity of Chinese marriage law and divorce narrative in the 1950s
Authors: Luo Yingyu , Han Chao
Published: February 2024

Abstract: After the promulgation of the Marriage Law of the People’s Republic of China in 1950, literary works engendered from the exigencies of publicity held distinct policy implications and disciplinary significance. Given that the issue of divorce encapsulates both the rationale of “free rights” and the sensibility of social disorder, the narrative of “divorce” during this period is, to some extent, situated between politics and reality. On the one hand, literary works must positively advocate the legal concept of “divorce freedom”, while, on the other hand, they must also undergo a delicately nuanced narrative processing to circumvent sensitivities. Consequently, the depiction of divorce narratives is characterized by ambiguity and capriciousness. Nonetheless, literature has to some extent bridged the gap between law and reality by reimagining the genuine predicaments triggered by divorce.
Confucian thought of “Harmony between man and nature” and contemporary society construction
Authors: Liu Yanling , Wu Yan
Published: February 2024

Abstract: In the ancient Confucian philosophy, the thought of “Harmony between Man and Nature” occupies a paramount position. The Confucian notion has a deep-rooted and extensive history, evolving and deepening over successive generations. Its contents are profound and intricate, encompassing a harmonious and integrated perspective on nature, a prioritization of righteousness over personal interest, and a sustainable development outlook. These ideas hold significant value as a point of reference for the establishment and advancement of contemporary social systems. This article aims to explore the origins and development of the concept of “Harmony between Man and Nature” while analyzing and reflecting on how this ideology influences the construction of present-day society.
O que está acontecendo? O que os trending topics podem nos dizer a respeito de ações políticas coletivamente orquestradas
Authors: Chagas Viktor
Published: February 2024

Este artigo se propõe a compreender as dinâmicas concernentes a ações políticas, executadas por meio de hashtags no Twitter. Seu principal objetivo é responder se o acompanhamento dos trending topics pode ajudar a identificar momentos de tensão política aguda. Com base em uma análise de fluxos de ranqueamento, a hipótese principal levantada por essa investigação é a de que, como tendências de uma opinião pública conectada, as trends podem operar como um termômetro sobre o clima de opinião nas redes. Além disso, é possível notar, com o uso de hashtags como marcadores de apoio e desarrimo, o emprego de táticas de astroturfing para alcançar visibilidade e repercussão nas mídias sociais. O estudo empreendeu uma coleta sistemática das principais trends no Brasil, entre janeiro e julho de 2019, a fim de avaliar estratégias relacionadas à capacidade concorrencial desses grupos.
Embates discursivos, atores envolvidos e polarização no Twitter: a demissão do Ministro da Educação Abraham Weintraub do governo Bolsonaro
Authors: Penteado Claudio Luis de Camargo , Pereira Marcus Abílio , Cervi Emerson Urizzi , Almeida Helga do Nascimento de , Rocha Bruno Anunciação , Chaves Julia Marks Santana
Published: February 2024

Este artigo apresenta um estudo das dimensões que envolvem os embates discursivos entre grupos de apoiadores políticos no debate público em plataformas digitais. Por meio do estudo de caso do embate no Twitter em torno da demissão de Weintraub do Ministério da Educação do governo Bolsonaro, apresenta uma análise que destaca três dimensões do embate discursivo: (a) a cronologia do comportamento das redes de interação discursivas sobre o tema; (b) as temáticas que foram mobilizadas; e (c) o perfil daqueles usuários que mais tuitaram durante as discussões. Os resultados apontam que os embates discursivos são dominados 1) pela replicação de mensagens originais (retweets), 2) pelo entrecruzamento de temas que envolvem os personagens e o contexto do conflito e 3) pela grande atividade de perfis não institucionais na difusão das mensagens associadas a seus posicionamentos ideológicos.