bluuebunny/crossref_metadata_2025 huggingface dataset:
A pilot study suggests the correspondence between SAR202 bacteria and dissolved organic matter in the late stage of a year-long microcosm incubation
Authors: Jia Yufeng , He Changfei , Lahm Madeline , Chen Qi , Powers Leanne , Gonsior Michael , Chen Feng
Published: April 2024

SAR202 bacteria are abundant in the marine environment and they have been suggested to contribute to the utilization of recalcitrant organic matter (RDOM) within the ocean’s biogeochemical cycle. However, this functional role has only been postulated by metagenomic studies. During a one-year microcosm incubation of an open ocean microbial community with lysed Synechococcus and its released DOM, SAR202 became relatively more abundant in the later stage (after day 30) of the incubation. Network analysis illustrated a high degree of negative associations between SAR202 and a unique group of molecular formulae (MFs) in phase 2 (day 30 to 364) of the incubation, which is empirical evidence that SAR202 bacteria are major consumers of the more oxygenated, unsaturated, and higher-molecular-weight MFs. Further investigation of the SAR202-associated MFs suggested that they were potentially secondary products arising from initial heterotrophic activities following the amendment of labile Synechococcus-derived DOM. This pilot study provided a preliminary observation on the correspondence between SAR202 bacteria and more resistant DOM, further supporting the hypothesis that SAR202 bacteria play important roles in the degradation of RDOM and thus the ocean’s biogeochemical cycle.
Aptly chosen, effectively emphasizing the action and mechanism of antimycin A1
Authors: Zhu Linyan , Weng Chenhong , Shen Xiaoman , Zhu Xiangdong
Published: April 2024

Rhizoctonia solani Kühn, a plant pathogenic fungus that can cause diseases in multiple plant species is considered one of the common and destructive pathogens in many crops. This study investigated the action of antimycin A1, which was isolated from Streptomyces AHF-20 found in the rhizosphere soil of an ancient banyan tree, on Rhizoctonia solani and its mechanism. The inhibitory effect of antimycin A1 on R. solani was assessed using the comparative growth rate method. The results revealed that antimycin A1 exhibited a 92.55% inhibition rate against R. solani at a concentration of 26.66 μg/mL, with an EC50 value of 1.25 μg/mL. To observe the impact of antimycin A1 on mycelial morphology and ultrastructure, the fungal mycelium was treated with 6.66 μg/mL antimycin A1, and scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. SEM analysis demonstrated that antimycin A1 caused mycelial morphology to become stripped, rough, and folded. The mycelium experienced severe distortion and breakage, with incomplete or locally enlarged ends, shortened branches, and reduced numbers. TEM observation revealed thickened cell walls, indistinct organelle boundaries, swollen mitochondria, exosmotic substances in vesicles, slow vesicle fusion, and cavitation. Real-time quantitative PCR and enzyme activity assays were conducted to further investigate the impact of antimycin A1 on mitochondria. The physiological and biochemical results indicated that antimycin A1 inhibited complexes III and IV of the mitochondrial electron transport chain. RT-PCR analysis demonstrated that antimycin A1 controlled the synthesis of relevant enzymes by suppressing the transcription levels of ATP6, ATP8, COX3, QCR6, CytB, ND1, and ND3 genes in mitochondria. Additionally, a metabolomic analysis revealed that antimycin A1 significantly impacted 12 metabolic pathways. These pathways likely experienced alterations in their metabolite profiles due to the inhibitory effects of antimycin A1. Consequently, the findings of this research contribute to the potential development of novel fungicides.
Physical activity may a probably protective factor for postoperative delirium: the PNDABLE study
Authors: Kong Jian , Lin Xu , Wang Bin , Xu Shanling , Wang Yuanlong , Hua Shuhui , Gong Hongyan , Dong Rui , Lin Yanan , Li Chuan , Bi Yanlin
Published: April 2024

Objective

This study aims to explore the relationship between physical activity (PA) and postoperative delirium (POD).

Methods

We selected 400 patients from the Perioperative Neurocognitive Disorder and Biomarkers Lifestyle (PNDABLE) database, and the patients in the PNDABLE database were sampled and tested Alzheimer’s biomarkers. The diagnosis of POD was made using the Confusion Assessment Scale (CAM) and the severity was assessed using Memorial Delirium Assessment Scale (MDAS). Mini-Mental State Examination (MMSE) scale was used to detect the mental state of the patients. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of preoperative cerebrospinal fluid (CSF) biomarkers, such as amyloid β plaque 42 (Aβ42), total tau protein (T-tau), and phosphorylated tau protein (P-tau). Logistic regression, sensitivity analysis, and post hoc analysis were used to explore the relationship between risk and protective factors on POD. We used the mediating effect to explore whether PA mediates the occurrence of POD through CSF biomarkers.

Results

The incidence of POD was 17.5%. According to our research, the consequence prompted that PA might be the protective factor for POD [odds ratio (OR): 0.336, 95% confidence interval (95 CI) 0.206–0.548, P < 0.001]. The result of logistic regression revealed that CSF biomarker Aβ42 (OR: 0.997, 95 CI 0.996–0.999, P < 0.001) might be a protective factor against POD, and the T-tau (OR: 1.006, 95 CI 1.003–1.009, P = 0.001) and P-tau (OR: 1.039, 95 CI 1.018–1.059, P < 0.001) might risk factors for POD. Sensitivity analysis confirmed the correlation between PA and CSF biomarkers in the patients with POD. Mediation effect analysis showed that PA may reduce the occurrence of POD partly through CSF biomarkers, such as Aβ42 (proportion: 11%, P < 0.05), T-tau (proportion: 13%, P < 0.05), and P-tau (proportion: 12%, P < 0.05).

Conclusion

Physical activity is probably a protective factor for POD and may exert a mediating effect through CSF biomarkers.
Selection of lansoprazole from an FDA-approved drug library to inhibit the Alzheimer’s disease seed-dependent formation of tau aggregates
Authors: Imtiaz Ahmed , Shimonaka Shotaro , Uddin Mohammad Nasir , Elahi Montasir , Ishiguro Koichi , Hasegawa Masato , Hattori Nobutaka , Motoi Yumiko
Published: April 2024

The efficacy of current treatments is still insufficient for Alzheimer’s disease (AD), the most common cause of Dementia. Out of the two pathological hallmarks of AD amyloid-β plaques and neurofibrillary tangles, comprising of tau protein, tau pathology strongly correlates with the symptoms of AD. Previously, screening for inhibitors of tau aggregation that target recombinant tau aggregates have been attempted. Since a recent cryo-EM analysis revealed distinct differences in the folding patterns of heparin-induced recombinant tau filaments and AD tau filaments, this study focused on AD seed-dependent tau aggregation in drug repositioning for AD. We screened 763 compounds from an FDA-approved drug library using an AD seed-induced tau aggregation in SH-SY5Y cell-based assay. In the first screening, 180 compounds were selected, 72 of which were excluded based on the results of lactate dehydrogenase assay. In the third screening with evaluations of soluble and insoluble tau, 38 compounds were selected. In the fourth screening with 3 different AD seeds, 4 compounds, lansoprazole, calcipotriene, desogestrel, and pentamidine isethionate, were selected. After AD seed-induced real-time quaking-induced conversion, lansoprazole was selected as the most suitable drug for repositioning. The intranasal administration of lansoprazole for 4 months to AD seed-injected mice improved locomotor activity and reduced both the amount of insoluble tau and the extent of phosphorylated tau-positive areas. Alanine replacement of the predicted binding site to an AD filament indicated the involvement of Q351, H362, and K369 in lansoprazole and C-shaped tau filaments. These results suggest the potential of lansoprazole as a candidate for drug repositioning to an inhibitor of tau aggregate formation in AD.
Assessing the perception of Parkinson’s disease in Al-Ahsa, Saudi Arabia among the visitors of a public campaign: before and after survey
Authors: Alokley Alia A. , Alhubail Fatimah M. , Al Omair Abdullah M. , Alturki Rawan A. , Alhaddad Rabab M. , Al Mousa Ali M. , Busbait Sarah A. , Alnaim Mohammed A.
Published: April 2024

Introduction

Insufficient knowledge and beliefs hinder the early diagnosis and provision of adequate treatment and care for PD patients, causing socio-economic burdens. Raising public awareness and understanding the knowledge gap is crucial for effective educational programs and early detection. This study aims to assess the perception of Parkinson’s disease (PD) among visitors of a public campaign conducted to raise PD awareness and knowledge to facilitate early detection and management.

Methods

A before-and-after study was conducted from May to June 2023, to assess the perception of PD among a public PD campaign visitors in Al-Ahsa, Saudi Arabia. The campaign included educational booths discussing PD symptoms, causes, diagnosis, management, and support. Participants completed a self-administered online questionnaire before and after the campaign. The data were analyzed using SPSS.

Results

The study included 48 eligible individuals. The mean knowledge score was significantly enhanced following the campaign, rising from 12 to 17.77 points (p < 0.05). The symptoms of PD that showed a significant difference were slow movement, tremors, depression, memory problems, and sleep problems. The campaign had a beneficial effect on participants’ general understanding of PD.

Discussion

The study showed that public awareness campaigns successfully raised community awareness of PD. Participants’ knowledge improved after the campaign, highlighting its positive effect. Further research could explore measuring the continuity of awareness over a longer period and its impact on improving patients’ lives and could expand the sample size.
Cerebellar ependymal cyst: a case report
Authors: Hou Chengye , Liu Yuanqin , Li Feng , Zhang Qinglu
Published: April 2024

Rationale

Intracranial ependymal cysts are relatively rare. The current case report focuses on a patient who was diagnosed with an ependymal cyst and underwent surgical treatment. Postoperative pathological examination confirmed the presence of this lesion in the cerebellum.

Chief complaint

A 32-year-old female patient presented with a chief complaint of dizziness and headache with no triggers for the past 1 year. She also reported an increase in the frequency and intensity of symptoms in the past 2 weeks.

Diagnosis

Cranial magnetic resonance imaging (MRI) showed a rounded long T1 and T2 abnormal signal foci in the left posterior part of the brainstem under the cerebellar pallidum. The lesion had a clear boundary, was approximately 4.0 × 3.1 × 3.2 cm in size, and did not exhibit any definitive enhancement.

Interventions

Total resection of the lesion was carried out after completion of the preoperative examination.

Treatment outcomes. The patient was discharged from the hospital on postoperative day 11 once their symptoms had disappeared. The sensory and motor functions of the limbs remained unaffected by treatment.

Experiences

Cerebellum ependymal cysts are rare, and most patients only experience discomfort due to cerebral edema. These lesions are also often difficult to differentiate from other intracranial cysts using imaging alone. The aim of this study was to report a rare case of ependymal cyst so that it may serve as a reference for diagnosis and treatment in the future.
Implementation of a roadmap for the comprehensive diagnosis, follow-up, and research of childhood leukemias in vulnerable regions of Mexico: results from the PRONAII Strategy
Authors: Núñez-Enríquez Juan Carlos , Romo-Rodríguez Rubí , Gaspar-Mendoza Pedro , Zamora-Herrera Gabriela , Torres-Pineda Lizeth , Amador-Cardoso Jiovanni , López-Blanco Jebea A. , Alfaro-Hernández Laura , López-García Lucero , Rosas-Cruz Arely , Alberto-Aguilar Dulce Rosario , Trejo-Pichardo César Omar , Ramírez-Ramírez Dalia , Cruz-Maza Astin , Flores-Lujano Janet , Luna-Silva Nuria , Martínez-Martell Angélica , Martínez-Jose Karina , Ramírez-Ramírez Anabel , Solis-Poblano Juan Carlos , Zagoya-Martínez Patricia , Terán-Cerqueda Vanessa , Huerta-Moreno Andrea , Montiel-Jarquín Álvaro , Garrido-Hernández Miguel , Hernández-Ramos Raquel , Olvera-Caraza Daniela , Cruz-Medina Cynthia Shanat , Alvarez-Rodríguez Enoch , Chávez-Aguilar Lénica Anahí , Herrera-Olivares Wilfrido , García-Hidalgo Brianda , Cano-Cuapio Lena Sarahí , Guevara-Espejel Claudia , Juárez-Avendaño Gerardo , Balandrán Juan Carlos , Baños-Lara Ma. del Rocío , Cárdenas-González Mariana , Álvarez-Buylla Elena R. , Pérez-Tapia Sonia Mayra , Casique-Aguirre Diana , Pelayo Rosana
Published: April 2024

The main objective of the National Project for Research and Incidence of Childhood Leukemias is to reduce early mortality rates for these neoplasms in the vulnerable regions of Mexico. This project was conducted in the states of Oaxaca, Puebla, and Tlaxcala. A key strategy of the project is the implementation of an effective roadmap to ensure that leukemia patients are the target of maximum benefit of interdisciplinary collaboration between researchers, clinicians, surveyors, and laboratories. This strategy guarantees the comprehensive management of diagnosis and follow-up samples of pediatric patients with leukemia, centralizing, managing, and analyzing the information collected. Additionally, it allows for a precise diagnosis and monitoring of the disease through immunophenotype and measurable residual disease (MRD) studies, enhancing research and supporting informed clinical decisions for the first time in these regions through a population-based study. This initiative has significantly improved the diagnostic capacity of leukemia in girls, boys, and adolescents in the regions of Oaxaca, Puebla, and Tlaxcala, providing comprehensive, high-quality care with full coverage in the region. Likewise, it has strengthened collaboration between health institutions, researchers, and professionals in the sector, which contributes to reducing the impact of the disease on the community.
VISTA/CTLA4/PD1 coexpression on tumor cells confers a favorable immune microenvironment and better prognosis in high-grade serous ovarian carcinoma
Authors: Jlassi Aida , Rejaibi Rim , Manai Maroua , Sahraoui Ghada , Guerfali Fatma Zahra , Charfi Lamia , Mezlini Amel , Manai Mohamed , Mrad Karima , Doghri Raoudha
Published: April 2024

Introduction

Immunotherapy by blocking immune checkpoints programmed death/ligand (PD1/PDL1) and cytotoxic T-lymphocyte-associated protein 4(CTLA4) has emerged as new therapeutic targets in cancer. However, their efficacy has been limited due to resistance. A new- checkpoint V-domain Ig-containing suppressor of T cell activation (VISTA) has appeared, but the use of its inhibition effect in combination with antibodies targeting PDL1/PD1and CTLA4 has not been reported in ovarian cancer.

Methods

In this study, we investigated the expressions of VISTA, CTLA4, and PDL1 using immunohistochemistry (IHC)on 135 Formalin-Fixed Paraffin-Embedded (FFPE)tissue samples of High-grade serous carcinoma (HGSOC). VISTA, CTLA4, PDL1, PD1, CD8, CD4, and FOXP3 mRNA extracted from 429 patients with ovarian cancer in the Cancer Genome Atlas (TCGA) database was included as a validation cohort. Correlations between these checkpoints, tumor-infiltrating- lymphocytes (TILs), and survival were analyzed.

Results and discussion

CTLA4 was detectable in 87.3% of samples, VISTA in 64.7%, PD1 in 56.7%, and PDL1 in 48.1%. PDL1 was the only tested protein associated with an advanced stage (p=0.05). VISTA was associated with PDL1, PD1, and CTLA4 expressions (p=0.005, p=0.001, p=0.008, respectively), consistent with mRNA level analysis from the TCGA database. Univariate analyses showed only VISTA expression (p=0.04) correlated with overall survival (OS). Multivariate analyses showed that VISTA expression (p=0.01) and the coexpression of VISTA+/CTLA4+/PD1+ (p=0.05) were associated with better OS independently of the clinicopathological features. Kaplan-Meier analysis showed that the coexpression of the VISTA+/CTLA4+/PDL1+ and VISTA+/CTLA4+/PD1+ checkpoints on tumor cells (TCs)were associated with OS (p=0.02 and p<0.001; respectively). VISTA+/CTLA4+/PD1+ in TCs and CD4+/CD8+TILswere associated with better 2-yer OS. This correlation may refer to the role of VISTA as a receptor in the TCs and not in the immune cells. Thus, targeting combination therapy blocking VISTA, CTLA4, and PD1 could be a novel and attractive strategy for HGSOC treatment, considering the ambivalent role of VISTA in the HGSOC tumor cells.
Case report: Characteristics and nature of primary cardiac synovial sarcoma
Authors: Kawasaki Tomonori , Nakajima Tomomi , Torigoe Tomoaki , Onohara Kojiro , Ishii Kentaro , Kanno Satoshi , Muramatsu Chisako , Tatsuno Rikito , Jubashi Takahiro , Ichikawa Jiro
Published: April 2024

Primary malignant cardiac tumors rarely occur, and cardiac synovial sarcoma (SS) is especially rare among such tumors. Herein, we present the case of a 35-year-old female with primary cardiac SS treated with surgery, chemotherapy, and radiotherapy. She presented with chest symptoms and underwent imaging examinations. A cardiac tumor was suspected, and an open biopsy was performed. The pathological findings suggested cardiac SS. Next, we performed a resection, and the tumors persisted at a macroscopic level. Immunohistochemistry was negative for SS18-SSX and positive for the SSX C-terminus and cytokeratin CAM5.2, a reduction of SMARCB1/INI1 was observed, and fluorescence in situ hybridization showed positive SS18 split staining. Owing to the FNCLCC grade 3 tumor and R2 margins, adjuvant chemotherapy with ifosfamide, doxorubicin, and radiotherapy was initiated, and the patient was diagnosed with cardiac SS. The differences in patients with cardiac SS compared with general SS include male predominance, larger tumor size, and poorer prognosis. Pathological findings of immunohistochemistry and fluorescence in situ hybridization were found to be more reliable than imaging findings for a correct diagnosis. Additionally, because incomplete resection is frequently performed, adjuvant therapy, including chemotherapy and radiation therapy, may be performed. The findings indicate that multiple therapies, including surgery, chemotherapy, and radiotherapy, are essential treatment strategies for improving the prognosis of patients with cardiac SS.
Managing venous thrombosis in a pediatric patient with short bowel and congenital nephrotic syndromes: a case report emphasizing rivaroxaban level monitoring
Authors: Bosch-Schips Marc , Artaza Gonzalo , Hernández-Mata Carlos , Pérez Beltrán Víctor , Cabello Ruiz Vanessa , Olivera Sumire Pável
Published: April 2024

Direct Oral Anticoagulants (DOACs) typically exhibit a predictable pharmacokinetic and pharmacodynamic response at a fixed dose, not necessitating monitoring under standard conditions. Yet, in specific clinical scenarios that can impair it, like Congenital Nephrotic Syndrome (CNS) or Short Bowel Syndrome (SBS) due to absorption issues, anti-thrombin III (AT-III) deficiency and non-selective proteinuria, adjusting the dosage to achieve appropriate plasma concentrations could prove beneficial. We report a 3-month-old female with catheter-related jugular thrombosis affected by CNS concomitant to SBS and failure of both treatments with heparin and warfarin, that was switched to dose-adjusted pediatric rivaroxaban. Rivaroxaban was adjusted to reach peak levels between 189 and 419 ng/ml and the lower trough levels between 6 and 87 ng/ml. Increasing doses were needed due to SBS related malabsorption but a complete permeabilization of the vein was achieved without bleeding complications. The use of anti-Xa adjusted rivaroxaban could be an alternative to improve anticoagulation and secondary thromboprophylaxis in pediatric patients SBS and an option to children with CNS.
Genome-wide analysis of the mulberry (Morus abla L.) GH9 gene family and the functional characterization of MaGH9B6 during the development of the abscission zone
Authors: Deng Jing , Ahmad Bilal , Deng Xuan , Fan Zelin , Liu Lianlian , Lu Xiuping , Pan Yu , Zha Xingfu
Published: April 2024

Plant glycoside hydrolase family 9 genes (GH9s) are widely distributed in plants and involved in a variety of cellular and physiological processes. In the current study, nine GH9 genes were identified in the mulberry and were divided into two subfamilies based on the phylogenetic analysis. Conserved motifs and gene structure analysis suggested that the evolution of the two subfamilies is relatively conserved and the glycoside hydrolase domain almost occupy the entire coding region of the GH9s gene. Only segmental duplication has played a role in the expansion of gene family. Collinearity analysis showed that mulberry GH9s had the closest relationship with poplar GH9s. MaGH9B1, MaGH9B6, MaGH9B5, and MaGH9B3 were detected to have transcript accumulation in the stalk of easy-to drop mature fruit drop, suggesting that these could play a role in mulberry fruit drop. Multiple cis-acting elements related to plant hormones and abiotic stress responses were found in the mulberry GH9 promoter regions and showed different activities under exogenous abscisic acid (ABA) and 2,4- dichlorophenoxyacetic acid (2,4-D) stresses. We found that the lignin content in the fruit stalk decreased with the formation of the abscission zone (AZ), which could indirectly reflect the formation process of the AZ. These results provide a theoretical basis for further research on the role of GH9s in mulberry abscission.
Unveiling orphan receptor-like kinases in plants: novel client discovery using high-confidence library predictions in the Kinase–Client (KiC) assay
Authors: Jorge Gabriel Lemes , Kim Daewon , Xu Chunhui , Cho Sung-Hwan , Su Lingtao , Xu Dong , Bartley Laura E. , Stacey Gary , Thelen Jay J.
Published: April 2024

Plants are remarkable in their ability to adapt to changing environments, with receptor-like kinases (RLKs) playing a pivotal role in perceiving and transmitting environmental cues into cellular responses. Despite extensive research on RLKs from the plant kingdom, the function and activity of many kinases, i.e., their substrates or “clients”, remain uncharted. To validate a novel client prediction workflow and learn more about an important RLK, this study focuses on P2K1 (DORN1), which acts as a receptor for extracellular ATP (eATP), playing a crucial role in plant stress resistance and immunity. We designed a Kinase-Client (KiC) assay library of 225 synthetic peptides, incorporating previously identified P2K phosphorylated peptides and novel predictions from a deep-learning phosphorylation site prediction model (MUsite) and a trained hidden Markov model (HMM) based tool, HMMER. Screening the library against purified P2K1 cytosolic domain (CD), we identified 46 putative substrates, including 34 novel clients, 27 of which may be novel peptides, not previously identified experimentally. Gene Ontology (GO) analysis among phosphopeptide candidates revealed proteins associated with important biological processes in metabolism, structure development, and response to stress, as well as molecular functions of kinase activity, catalytic activity, and transferase activity. We offer selection criteria for efficient further in vivo experiments to confirm these discoveries. This approach not only expands our knowledge of P2K1’s substrates and functions but also highlights effective prediction algorithms for identifying additional potential substrates. Overall, the results support use of the KiC assay as a valuable tool in unraveling the complexities of plant phosphorylation and provide a foundation for predicting the phosphorylation landscape of plant species based on peptide library results.
Slovakia and its environmental transformation: measuring environmental attitudes using the new ecological paradigm
Authors: Marcineková Lenka , Štěrbová Martina , Výbošťok Jozef , Hajdúchová Iveta , Giertliová Blanka , Šulek Rastislav , Sarvašová Zuzana , Šálka Jaroslav
Published: April 2024

Many scientists have studied the relationship between society and the environment. The New Ecological Paradigm (NEP) has been repeatedly used for the analysis of public environmental attitudes, as the public pays increased attention to the environmental issues, sustainability, or the climate crisis. Our paper deals with the use of the NEP to study and identify public environmental attitudes in the Slovak Republic. We discovered a deviation of our results from the NEP, as we identified altered environmental attitudes, which we find acceptable, as environmental attitudes are a delicate set of values encompassing different, yet interrelated facets. According to our results, we were able to classify our respondents into those with technocentric, ecocentric, and resiliocentric attitudes, while up to 70% of respondents hold the ecocentric attitude. These findings could be influenced by the fact that no significant changes in the population’s lifestyle have been required up to now. The NEP has proven to be an important predictive tool in assessing public environmental attitudes to determine readiness for environmental transformation. Nevertheless, we recommend using a combination of analysis that includes evaluating the impact of demographic factors to achieve higher-quality results.
Educators as agents of breadth-biased learning: using social reconstructionism as rationale for embracing media multitasking and enhancing teaching practices in higher education
Authors: Kassie Seada A.
Published: April 2024

This perspective article contends that media multitasking has significant implications on cognitive control processes, particularly in how information is processed and utilized. Contrary to viewing media multitasking as inherently negative, the article argues that it contributes to the evolving nature of cognitive processing, without necessarily improving or degrading it. The discussion draws on theoretical frameworks from contemporary cognitive neuroscience to contextualize these arguments. The article provides a nuanced perspective on media multitasking, acknowledging its enduring presence and exploring its influence on cognitive processes, while also proposing strategies for educators to navigate its implications in educational settings.
Serious adverse drug events associated with psychotropic treatment of bipolar or schizoaffective disorder: a 17-year follow-up on the LiSIE retrospective cohort study
Authors: Truedson Petra , Ott Michael , Wahlström Lisa , Lundqvist Robert , Maripuu Martin , Lindmark Krister , Lieber Ingrid , Werneke Ursula
Published: April 2024

Introduction

Mood stabilisers and other psychotropic drugs can lead to serious adverse drug events (ADEs). However, the incidence remains unknown. We aimed to (a) determine the incidence of serious ADEs in patients with bipolar or schizoaffective disorders, (b) explore the role of lithium exposure, and (c) describe the aetiology.

Methods

This study is part of the LiSIE (Lithium—Study into Effects and Side Effects) retrospective cohort study. Between 2001 and 2017, patients in the Swedish region of Norrbotten, with a diagnosis of bipolar or schizoaffective disorder, were screened for serious ADEs to psychotropic drugs, having resulted in critical, post-anaesthesia, or intensive care. We determined the incidence rate of serious ADEs/1,000 person-years (PY).

Results

In 1,521 patients, we identified 41 serious ADEs, yielding an incidence rate of 1.9 events per 1,000 PY. The incidence rate ratio (IRR) between ADEs with lithium present and causally implicated and ADEs without lithium exposure was significant at 2.59 (95% CI 1.20–5.51; p = 0.0094). The IRR of ADEs in patients <65 and ≥65 years was significant at 3.36 (95% CI 1.63–6.63; p = 0.0007). The most common ADEs were chronic lithium intoxication, oversedation, and cardiac/blood pressure-related events.

Discussion

Serious ADEs related to treatment of bipolar (BD) or schizoaffective disorder (SZD) were uncommon but not rare. Older individuals were particularly at risk. The risk was higher in individuals exposed to lithium. Serum lithium concentration should always be checked when patients present with new or unclear somatic symptoms. However, severe ADEs also occurred with other mood stabilisers and other psychotropic drugs.
Increased eating disorder frequency and body image disturbance among fashion models due to intense environmental pressure: a content analysis
Authors: Bogár Nikolett , Kővágó Pál , Túry Ferenc
Published: April 2024

Introduction

Female fashion models are under intense occupational pressure. The present study focuses on assessing the lived experience of fashion models with regards to their dieting and exercising habits, body image perception, eating disorder-like symptoms, and experience of abuse via self-narrated reports.

Methods

Series of open questions were distributed among international fashion models (N=84, mean age=23.2 years; mean BMI=16.9) selected by convenience and snowball sampling. Models from 17 countries participated. The questions targeted models’ eating, exercising, dieting habits, body image perception, and eating disorder symptoms. The average word count of the transcripts was 2473.9 (SD = 2791.6). Thematic content analysis was performed on the transcripts. A total of 31 codes were created to address disordered eating and body image concerns.

Results

Negative body-related claims appeared in 89.3%, and positive claims in 64.3% of the models’ transcripts. Negative remarks about eating were made by 45.2% of the participants, and 23.8% positively. Control over their food intake was exercised by 78.6% of the participants and 40.5% used extreme calorie restriction. Models who talked more positively about their bodies expressed significantly more frequently extreme calorie restriction. Extreme sports habits occurred in 23.8% of the transcripts, obsessive sports habits were claimed by 11.9% of participants. Self-induced vomiting was prominent in 14.3% of the answers. Criticism from other industry members was experienced by 83.3% of the participants while 44.0% received body appreciation. Body image disorder-like symptoms were expressed by 63.1% of models. Such models mentioned significantly more often content about eating disorders and talked significantly more negatively about eating. Psychological problems were mentioned by 48.8%, whereas 16.7% took part in psychotherapy. Those who partake in therapy mentioned significantly more eating disorder content in their narratives. Abuse was mentioned by 25.0% of the models.

Conclusion

Fashion models are experiencing increased environmental pressure to conform to the extreme slimness ideal. There is a heightened prevalence of disordered eating and other weight-controlling behaviours among fashion models to succeed in their careers. Qualitative research is crucial in understanding the more subtle dynamics in conforming to and maintaining the thin beauty ideal.
Effectiveness of interventions to improve adherence to antidepressant medication in patients with depressive disorders: a cluster randomized controlled trial
Authors: del Pino-Sedeño Tasmania , González-Pacheco Himar , González de León Beatriz , Serrano-Pérez Pedro , Acosta Artiles Francisco Javier , Valcarcel-Nazco Cristina , Hurtado-Navarro Isabel , Rodríguez Álvarez Cristobalina , Trujillo-Martín María M. ,   on behalf of MAPDep Team
Published: April 2024

Aim

To assess the effectiveness of two interventions of knowledge transfer and behavior modification to improve medication adherence in patients with depressive disorders.

Methods

An open, multicenter, three-arm clinical trial with random allocation by cluster to usual care or to one of the two interventions. The intervention for psychiatrists (PsI) included an educational program based on a patient-centered care model. The intervention for patients and relatives (PtI) included a collaborative care program plus a reminder system that works using an already available medication reminder application. The primary outcome was patient adherence to antidepressant treatment assessed through the Sidorkiewicz Adherence Instrument. Secondary measures were depression severity, comorbid anxiety and health-related quality of life. Mixed regression models with repeated measures were used for data analysis.

Results

Ten psychiatrists and 150 patients diagnosed with depressive disorder from eight Community Mental Health Units in the Canary Islands (Spain) were included. Compared with usual care, no differences in long-term adherence were observed in either group PsI or PtI. The PsI group had significantly improved depression symptoms (B = −0.39; 95%CI: −0.65, −0.12; p = 0.004) during the follow-up period. The PtI group presented improved depression symptoms (B = −0.63; 95%CI: −0.96, −0.30; p < 0.001) and mental quality of life (B = 0.08; 95%CI: 0.004, 0.15; p = 0.039) during the follow-up period.

Conclusion

The assessed interventions to improve adherence in patients with depressive disorder were effective for depression symptoms and mental quality of life, even over the long term. However, no effect on antidepressant adherence was observed.
The relationship between health insurance and economic performance: an empirical study based on meta-analysis
Authors: Fan Chenchen , Li Chunyan , Song Xiaoting
Published: April 2024

Health insurance stands as a pivotal facet of social wellbeing, with profound implications for the overarching landscape of economic development. The existing research, however, lacks consensus on the relationship between health insurance and economic performance and provides no evidence about the magnitude of the correlation. This lack of information seriously impedes the high-quality development of the healthcare system. Therefore, to scientifically elucidate the relationship between the two, this study involved a meta-analysis, analyzing 479 effect values derived from 34 independent research samples. The results reveal a strongly positive correlation between health insurance and economic performance [r = 0.429, 95% CI = (0.381, 0.475)]. Findings show that health insurance in developed countries more effectively fosters economic performance than in developing countries. Moreover, public health insurance exerts a stronger promoting effect on economic performance than commercial health insurance. The relationship between health insurance and economic performance is moderated by data type, research method, country of sample origin, literature type, journal impact factor, publication year, type of health insurance, and the research populations. Based on meta-analysis, this study not only scientifically responds to the controversy of the relationship between health insurance and economic performance, and the magnitude of a correlation, but also further reveals the inner conduction mechanism between the two. Our research findings are meaningful for policymakers to choose an appropriate healthcare strategy according to their unique attributes, propelling sustainable economic development.
Health risk assessment of ciprofloxacin, tetracycline, and oxytetracycline residues in raw, frozen, and boiled broiler chicken available in a local area of Bangladesh
Authors: Haque Shaila , Jamil Md. Yusuf , Khan Md. Shahinul Haque , Reza Md. Sajib Al , Esrafil Md. , Abedin Md. Zainul , Zubair Md. Abu , Sikder Md. Asaduzzaman , Bari Luthfunnesa
Published: April 2024

Introduction

The misuse of antibiotics in poultry farming is a global issue.

Objective

The focus of this study was the health risk assessment of consumers from the determination of ciprofloxacin (CIP), tetracycline (TC), and oxytetracycline (OTC) in broiler chicken in the raw, frozen, and boiled stages using solid-phase extraction, high-performance liquid chromatography, and ultraviolet detection (SPE-HPLC-UV).

Materials and methods

Chromatographic separation was achieved using 0.3% metaphosphoric acid and acetonitrile (1:10, v/v) for CIP at 280 nm and oxalic acid (0.01 M) and acetonitrile (1:1, v/v) for TC and OTC at 355 nm with different retention times. The method had an acceptable precision with good linearity, specificity, limit of detection, limit of quantification, accuracy, and stability.

Results

Among a total of 252 raw samples, approximately 68.25%, 25.4%, and 7.54% contained CIP, TC, and OTC, respectively. Out of the positive raw samples, CIP exceeded the maximum residual limit (MRL) in 3.6% muscle, 14.3% liver and 17.9% skin samples, whereas TC and OTC were below the MRLs. The residual concentrations of these antibiotics were almost unchanged in frozen samples. After boiling the chicken samples, the TC and OTC residues were reduced significantly compared to CIP. Although the concentrations of CIP in boiled samples were above the MRL set by the European Union, these did not exceed the hazard index 1.

Conclusion

Based on these results, the exposure levels to antibiotics in broiler chicken meats may be considered to have a low risk for human health.
Range and trophic niche overlap of two sympatric species of floral oil collecting bees in a fragment of dry forest
Authors: Martins Herbeson O. J. , Dórea Marcos C. , Ferreira Vinina S. , Silva Cláudia I. , Alves-dos-Santos Isabel
Published: April 2024

Plant-pollinator interactions constitute complex mutualistic relationships responsible for the maintenance of the reproduction of flowering plants in different biomes. The genus Centris stands out as one of the main groups of pollinators in the Neotropical region. These bees have a close relationship with floral oil-producing plants, using this resource in the construction of their nests and/or larval feeding. The aim of the present study was to compare the resources exploited by Centris perforator and Centris xanthomelaena and assess the established trophic relationships of these two species in a fragment of dry forest (Caatinga domain). For such, direct observations were made at the flowers and analyses were performed of the pollen load on the body of female bees and the content of the larval provisions in the nests. A strong association was found between the two bee species and plants with poricidal anthers of the genus Chamaecrista, which accounted for more than half of the larval diet in both species. Samples of the pollen load of C. xanthomelaena exhibited a greater diversity of pollen types. While Rhaphiodon echinus was a constant source of nectar for C. xanthomelaena, C. perforator females collected nectar from plants of short, rapid flowering, such as Cenostigma microphyllum and Tabebuia aurea. Malpighia emarginata was the main source of floral oil for both species. However, C. xanthomelaena also interacted with Angelonia cornigera and Krameria sp. to obtain this resource. The similarity in the trophic niche of the species was 57% in the subsamples of pollen on the body of females and 46% for larval provision. The results showed that areas of native vegetation are essential for bee populations in regions with agricultural activity, ensuring a regular supply of ecological resources for these bees.
Sleep/wake regularity influences how stress shapes executive function
Authors: Gilmore Gabriel R. , Smith Anna L. , Dickinson Fallon B. , Crosswell Alexandra D. , Mendes Wendy Berry , Whitehurst Lauren N.
Published: April 2024

Introduction

Sleep and stress processes shape executive function. Evidence suggests that poor sleep regulation can lead to significant impairments in executive functions. Psychological stress can also directly impact a variety of executive functions, often leading to declines, but may additionally reduce executive function via its negative impact on sleep. Rates of perceived stress and poor sleep have skyrocketed in recent years. As such, it has become increasingly important to understand how daily stress exposures and sleep processes modulate executive functions.

Methods

In a remote 21-day app-based study, 227 participants completed sleep and stress assessments three times a day. They also completed three executive functioning tasks at various timepoints across the 21-day study interval that assessed cognitive inhibition (Emotional Stroop task), cognitive flexibility (Trail Making Test A and B), and working memory (Backwards Digit Span).

Results

Participants with consistent sleep/wake schedules reported fewer acute stress events when compared to those with inconsistent sleep schedules. Those with greater sleep/wake regularity also had faster responses to self-relevant negative probes (vs. general negative and neutral) in the Stroop task. Further, variability in sleep/wake timing and reported acute stress exposures across the 21-day study interval interacted to predict performance on the Emotional Stroop task. Specifically, as the number of acute stress events experienced across the 21-day interval increased, participants with more regular sleep schedules had slower overall response times on the Stroop. Higher acute stress exposures led to specific response time delays to neutral and self-relevant negative probes for those with high sleep/wake regularity. We found no impact of the number of acute stress events or stress intensities on working memory span, Stroop accuracy, or Trails response time.

Discussion

These data may indicate that sleep/wake regularity preserves adaptive inhibitory control responses to cumulative acute stress.
PROSPECT: A comprehensive sample acquisition and analysis package for lunar science and exploration
Authors: Trautner R. , Barber S. J. , Fisackerly R. , Heather D. , Houdou B. , Howe C. , Iacobellis S. , Leese M. , Mariani A. , Meogrossi G. , Murray N. , Panza C. , Reiss P. , Rusconi A. , Abernethy F. , Cann N. , Chinnery H. , Gscheidle C. , Landsberg P. , Lindner R. , Morse A. D. , Mortimer J. , Nicolae L. , Picchi P. , Sheridan S. , Verchovsky A.
Published: April 2024

PROSPECT is a comprehensive payload package developed by the European Space Agency which will support the extraction and analysis of lunar surface and subsurface samples as well as the acquisition of data from additional environmental sensors. The key elements of PROSPECT are the ProSEED drill and the ProSPA analytical laboratory. ProSEED will support the acquisition of cryogenic samples from depths up to 1 m and deliver them to the ProSPA instrument. ProSPA will receive and seal samples in miniaturized ovens, heat them, physically and chemically process the released volatiles, and analyze the obtained constituents via mass spectrometry using two types of spectrometers. Contextual information will be provided by cameras which will generate multi-spectral images of the drill working area and of acquired samples, and via temperature sensors and a permittivity sensor that are integrated in the drill rod. The package is designed for minimizing volatile loss from the sample between acquisition and analysis. Initially developed for a flight on the Russian Luna-27 mission, the payload package design was adapted for a more generic lander accommodation and will be flown on a lunar polar lander mission developed within the NASA Commercial Lunar Payload Services (CLPS) program. PROSPECT targets science and exploration in lunar areas that might harbor deposits of volatiles, and also supports the demonstration of In-Situ Resource Utilization (ISRU) techniques in the lunar environment. PROSPECT operations are designed to be automated to a significant degree but rely on operator monitoring during critical phases. Here, we report the PROSPECT flight design that will be built, tested, and qualified according to European space technology engineering standards before delivery to the lander provider for spacecraft integration. The package is currently in the hardware manufacturing and integration phase with a target delivery to the NASA-selected CLPS lander provider in 2025.
TabNet: Locally Interpretable Estimation and Prediction for Advanced Proton Exchange Membrane Fuel Cell Health Management
Authors: Zhang Benyuan , Jin Xin , Liang Wenyu , Chen Xiaoyu , Li Zhenhong , Panoutsos George , Liu Zepeng , Tang Zezhi
Published: April 2024

In the pursuit of advanced Predictive Health Management (PHM) for Proton Exchange Membrane Fuel Cells (PEMFCs), conventional data-driven models encounter considerable barriers due to data reconstruction resulting in poor data quality, and the complexity of models leading to insufficient interpretability. In addressing these challenges, this research introduces TabNet, a model aimed at augmenting predictive interpretability, and integrates it with an innovative data preprocessing technique to enhance the predictive performance of PEMFC health management. In traditional data processing approaches, reconstruction methods are employed on the original dataset, significantly reducing its size and consequently diminishing the accuracy of model predictions. To overcome this challenge, the Segmented Random Sampling Correction (SRSC) methodology proposed herein effectively eliminates noise from the original dataset whilst maintaining its effectiveness. Notably, as the majority of deep learning models operate as black boxes, it becomes challenging to identify the exact factors affecting the Remaining Useful Life (RUL) of PEMFCs, which is clearly disadvantageous for the health management of PEMFCs. Nonetheless, TabNet offers insights into the decision-making process for predicting the RUL of PEMFCs, for instance, identifying which experimental parameters significantly influence the prediction outcomes. Specifically, TabNet’s distinctive design employs sequential attention to choose features for reasoning at each decision-making step, not only enhancing the accuracy of RUL predictions in PEMFC but also offering interpretability of the results. Furthermore, this study utilized Gaussian augmentation techniques to boost the model’s generalization capability across varying operational conditions. Through pertinent case studies, the efficacy of this integrated framework, merging data processing with the TabNet architecture, was validated. This work not only evidences that the effective data processing and strategic deployment of TabNet can markedly elevate model performance but also, via a visual analysis of the parameters’ impact, provides crucial insights for the future health management of PEMFCs.
Assessing the Feasibility of Biorefineries for a Sustainable Citrus Waste Management in Korea
Authors: Lee Sang-Hwan , Park Seong Hee , Park Hyun
Published: April 2024

Citrus fruits are one of the most widely used fruits around the world and are used as raw fruits, but are also processed into products such as beverages, and large amounts of by-products and waste are generated in this process. Globally, disposal of citrus waste (CW) through simple landfilling or ocean dumping can result in soil and groundwater contamination, which can negatively impact ecosystem health. The case of Korea is not much different in that these wastes are simply buried or recycled wastes are used as livestock feed additives. However, there are many reports that CW, which is a waste, has high potential to produce a variety of products that can minimize environmental load and increase added value through appropriate waste management. In this study, we aim to explore the latest developments in the evaluation and valorization of the growing CW green technologies in an effort to efficiently and environmentally transform these CW for resource recovery, sustainability, and economic benefits. Recent research strategies on integrated biorefinery approaches have confirmed that CW can be converted into various bioproducts such as enzymes, biofuels and biopolymers, further contributing to energy security. It was found that more efforts are needed to scale up green recovery technologies and achieve diverse product profiling to achieve zero waste levels and industrial viability.