bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Comparison of Three Intraoral Scanners for Implant—Supported Dental Prostheses
Authors: Costa Vitória , Silva António Sérgio , Costa Rosana , Barreiros Pedro , Mendes Joana , Mendes José Manuel
Published: June 2022

With continuing technological developments, there have been advances in the field of fixed prosthetics, particularly in impression-taking techniques. These technological advances mean that a wide variety of diagnostic and/or rehabilitation possibilities can be explored without the need for physical models. The aim of this study was to evaluate the accuracy of three intraoral scanners used in oral implant rehabilitation using an extraoral scanner as a reference and varying the scanning area. Three models representing different clinical scenarios were scanned 15 times by each intraoral scanner and three times by the extraoral scanner. The readings were analyzed and overlaid using engineering software (Geomagic® Control X software (Artec Europe, Luxembourg)). Statistically significant differences in accuracy were found between the three intraoral scanners, iTero® (Align Technology Inc., San Jose, CA, USA), Medit® (Medit®: Seoul, Korea), and Planmeca® (Planmeca®: Helsinki, Finland). In all clinical scenarios, the iTero® scanner had the best trueness (24.4 μm), followed by the Medit® (26.4 μm) and Planmeca® (42.1 μm). The Medit® showed the best precision (18.00 μm) followed by the iTero® (19.20 μm) and Planmeca® (34.30 μm). We concluded that the iTero® scanner had the highest reproducibility and accuracy in the clinical setting.
Novel Optimization Design Methods of Highly Loaded Compressor Cascades Considering Endwall Effect
Authors: Liu Bo , Chen Qidong , Li Jun , Mao Xiaochen
Published: June 2022

The endwall effect has a great impact on the aerodynamic performance of compressor blades. Based on three conventional near-endwall blade modeling methods of bowed blade, endbend blade and leading-edge strake blade (LESB), two combined optimization design methods of highly loaded blades have been developed considering the endwall effect in the current study, i.e., the bowed blade combined with the LESB (bowed LESB blade) and the endbend blade combined with the LESB (endbend LESB blade). Optimization designs were conducted for a compressor cascade with low solidity by using the two combined modeling methods and the three conventional modeling methods, and the optimization results were compared and analyzed in detail. The results showed that the five optimization modelling methods could all improve the performance for the original cascade, and the optimized cascade with the bowed LESB modeling method has the best aerodynamic performance. The total pressure loss of the optimal bowed LESB cascade was only 40.3% of that in the original cascade while reducing the solidity of the original cascade from 1.53 to 1.25 and keeping the static pressure rise and diffusion factor at the same level as the original one. Among the optimal cascades, the radial migration height of the low-energy fluid and the corresponding vortex have great effects on the aerodynamic performance, and the optimal bowed LESB cascade is superior to the other optimal cascades in this aspect.
A Novel Method for On-Line Characterization of Alkali Release and Thermal Stability of Materials Used in Thermochemical Conversion Processes
Authors: Andersson Viktor , Ge Yaxin , Kong Xiangrui , Pettersson Jan B. C.
Published: June 2022

Alkali metal compounds are released during the thermal conversion of biofuels and fossil fuels and have a major impact on the efficiency of conversion processes. Herein, we describe a novel method for the simultaneous characterization of alkali release and mass loss from materials used in combustion and gasification processes including solid fuels, fluidized bed materials, and catalysts for gas reforming. The method combines the thermogravimetric analysis of selected samples with the on-line measurement of alkali release using a surface ionization detector. The technique builds on the careful treatment of alkali processes during transport from a sample to the downstream alkali monitor including the losses of alkali in the molecular form to hot walls, the formation of nanometer-sized alkali-containing particles during the cooling of exhaust gases, aerosol particle growth, and diffusion losses in sampling tubes. The performance of the setup was demonstrated using biomass samples and fluidized bed material from an industrial process. The emissions of alkali compounds during sample heating and isothermal conditions were determined and related to the simultaneous thermogravimetric analysis. The methodology was concluded to provide new evidence regarding the behavior of alkali in key processes including biomass pyrolysis and gasification and ash interactions with fluidized beds. The implications and further improvements of the technique are discussed.
Load Frequency Robust Control Considering Intermittent Characteristics of Demand-Side Resources
Authors: Ming Guoxin , Geng Jian , Liu Jiantao , Chen Yiyuan , Yuan Kun , Zhang Kaifeng
Published: June 2022

Renewable energy has the characteristics of low carbon and environmental protection compared to traditional water and thermal power, but it also has the intermittency and uncertainty that traditional water and thermal power does not have. These characteristics make the inertia of the power system increase, which greatly affects the frequency stability of the grid. To solve such problems, the participation of demand-side resources (DSRs) in the dispatch of power systems has become a viable solution.However, unlike generation-side resources, DSRs have their own unique characteristics. In this paper, by taking into account a load frequency control system (LFC) with intermittent demand-side resources, the robust H∞ load frequency control problem are discussed in detail.A robust controller to coordinate the load side with the resource side is introduced. A critical stability criterion and robust performance evaluation of the new LFC system was carried out. Finally, simulation results based on the new LFC system are provided to demonstrate that the proposed control strategy can effectively improve the stability and robustness of the grid under large disturbances, thus allowing the grid frequency to return to the reference value.
Intelligent Tuned Hybrid Power Filter with Fuzzy-PI Control
Authors: Lin Tzu-Chiao , Simachew Bawoke
Published: June 2022

With the growing impact of power regulators, machines, and other power electronic devices on the quality of utility electric service, harmonics has recently emerged as a major research topic in the area of power quality and energy consumption. In this work, a fuzzy intelligent inductor–capacitor–inductor hybrid power filter is designed to reduce harmonics in the distribution line. The shunt active power filter and passive filter design and consideration have been improved. Since the nature of proportional integral control is only efficient with linear variations and the real condition of the distribution system is always varying nonlinearly, to create a minimum steady error, fuzzy-tuned proportional integral control is designed to intelligently consider the input changes and revise the decision rules using fuzzy reasoning. The content of the harmonic current with respect to the varying load is momentarily commutated. Thus, using Clarke’s transformation, the errors are summed with instantaneous power that is converted to a compensation current with the hysteresis band monitored pulse generator. The output current would turn off and on the IGBT inverter’s switches, supplying the current to reject the harmonics. The overall work was tested using MATLAB/SIMULINK® and was effective in canceling harmonics.
How to Reintroduce Arable Crops after Growing Perennial Wild Plant Species Such as Common Tansy (Tanacetum vulgare L.) for Biogas Production
Authors: von Cossel Moritz
Published: June 2022

The cultivation of perennial wild plant mixtures (WPMs) is becoming increasingly important in Germany for providing sustainably produced bioenergy. However, perennial energy cropping systems always raise the question of how to reclaim the land for arable crops. This study examined this issue by looking at how a former WPM area was returned to arable cropping for an organic farm. From 2013 to 2018, the WPM area was harvested annually in the autumn. From 2019 to 2020, it was co-managed with the surrounding land as a semi-intensive grassland under a three-cut regime. The area was then ploughed in the spring of 2021 to grow silage maize. Weeds were controlled mechanically once. Nevertheless, the perennial wild plant species grew vigorously, with common tansy (Tanacetum vulgare L.) standing out with a total fresh matter share of 29.0%. This maize–WPM mixture achieved a dry matter yield of 15.5 ± 5.5 Mg ha−1, which was notably but not significantly (p < 0.05) lower than that of silage maize growing next to the former WPM area (23.4 ± 5.5 Mg ha−1). After silage maize, winter wheat was sown in the autumn of 2021 and further regrowth of common tansy was observed in the spring of 2022. Yield and quality effects must therefore be given special consideration in the first arable crop following WPM cultivation.
UCB-SEnMod: A Model for Analyzing Future Energy Systems with 100% Renewable Energy Technologies—Methodology
Authors: Blinn Alexander , te Heesen Henrik
Published: June 2022

While the contribution of renewable energy technologies to the energy system is increasing, so is its level of complexity. In addition to new types of consumer systems, the future system will be characterized by volatile generation plants that will require storage technologies. Furthermore, a solid interconnected system that enables the transit of electrical energy can reduce the need for generation and storage systems. Therefore, appropriate methods are needed to analyze energy production and consumption interactions within different system constellations. Energy system models can help to understand and build these future energy systems. However, although various energy models already exist, none of them can cover all issues related to integrating renewable energy systems. The existing research gap is also reflected in the fact that current models cannot model the entire energy system for very high shares of renewable energies with high temporal resolution (15 min or 1-h steps) and high spatial resolution. Additionally, the low availability of open-source energy models leads to a lack of transparency about exactly how they work. To close this gap, the sector-coupled energy model (UCB-SEnMod) was developed. Its unique features are the modular structure, high flexibility, and applicability, enabling it to model any system constellation and can be easily extended with new functions due to its software design. Due to the software architecture, it is possible to map individual buildings or companies and regions, or even countries. In addition, we plan to make the energy model UCB-SEnMod available as an open-source framework to enable users to understand the functionality and configuration options more easily. This paper presents the methodology of the UCB-SEnMod model. The main components of the model are described in detail, i.e., the energy generation systems, the consumption components in the electricity, heat, and transport sectors, and the possibilities of load balancing.
New Approaches for the Fermentation of Beer: Non-Saccharomyces Yeasts from Wine
Authors: Postigo Vanesa , Sánchez Ana , Cabellos Juan Mariano , Arroyo Teresa
Published: June 2022

Non-Saccharomyces yeasts represent a very attractive alternative for the production of beers with superior sensory quality since they are able to enhance the flavour of beer. Furthermore, they can produce beers with low ethanol content due to the weak fermentative capacity of a large percentage of non-Saccharomyces species. The objective of this study was to evaluate the ability of 34 non-Saccharomyces yeast strains isolated from Madrilenian agriculture to produce a novel ale beer. The non-Saccharomyces yeast strains were screened at two scales in the laboratory. In the first screening, those with undesirable aromas were discarded and the selected strains were analysed. Thirty-three volatile compounds were analysed by GC, as well as melatonin production by HPLC, for the selected strains. Thirteen strains were then fermented at a higher scale in the laboratory for sensory evaluation. Only yeast strains of the species Schizosaccharomyces pombe and Lachancea thermotolerans were able to complete fermentation. Species such as Torulaspora delbrueckii, Metschnikowia pulcherrima, Wickerhamomyces anomalus, Hanseniaspora vineae, and Hanseniaspora guilliermondii could be used both for production of low ethanol beers and co-fermentation with a Saccharomyces yeast to improve the organoleptic characteristics of the beer. In addition, for these strains, the levels of melatonin obtained were higher than the concentrations found for Saccharomyces strains subjected to the same study conditions. The selected strains can be used in future trials to further determine their viability under different conditions and for different purposes.
Preparation and Properties of Blended Composite Film Manufactured Using Walnut-Peptide–Chitosan–Sodium Alginate
Authors: Yan Wenqi , Sun Haochen , Liu Wenxin , Chen Hao
Published: June 2022

In this study, layer-by-layer assembly was performed to prepare sodium alginate (SA) layer and walnut-peptide–chitosan (CS) bilayer composite films. Genipin was adopted to crosslink CS and walnut peptide. The properties of walnut peptide-CS-SA composite film were determined, and the influence of material ratio on the performance of composite film was explored. According to the results, the mechanical tensile property, oil absorption property, and water vapor barrier property of the composite film were improved with the presence of genipin. Moreover, the proportion of CS and walnut peptide had significant effects on color, transmittance, mechanical properties, barrier properties, and antioxidant properties of the composite films. Among them, the composite film containing 1% (w/v) CS, 1% (w/v) walnut peptide, and 0.01% (w/v) genipin showed the best performance, with a tensile strength of 3.65 MPa, elongation at break of 30.82%, water vapor permeability of 0.60 g·mm·m−2·h−1·kPa−1, oil absorption of 0.85%, and the three-phase electrochemistry of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging rate of 25.59%. Under this condition, the tensile property, barrier property, and oxidation resistance of the composite film are good, which can provide a good preservation effect for food, and has great application potential.
Effect of Microwave Pretreatment on the Antioxidant Activity and Stability of Enzymatic Products from Milk Protein
Authors: Yang Xue , Ren Xiaofeng , Ma Haile
Published: June 2022

The effects of microwave pretreatment on the antioxidant activity and stability of enzymatic products from milk protein (MP) were studied. The peptide content, molecular weight distribution, and amino acid composition of MP hydrolysate were also measured to explain the change of antioxidant activity under microwave pretreatment. The results showed that microwave pretreatment increased the degree of hydrolysis of MP with the power of 400 W for the highest value. The DPPH scavenging activity and the total antioxidant capacity of MP pretreated by microwave with a power of 300 W presented the highest effect and increased by 53.97% and 16.52%, respectively, compared to those of control. In addition, the results of thermal stability and in vitro digestion of MP hydrolysate showed that the MP hydrolysate pretreated by microwave exerted excellent antioxidative stability, especially for the microwave power of 300 W. After pretreated with microwave, the peptide content increased as the rise of power and it reached the peak at the power of 400 W. The molecular weight of MP hydrolysate pretreated by microwave with the power of 300 W showed more percentage of peptides between 200 Da and 500 Da. The result of amino acid composition showed that total amino acid (TAA) content of MP hydrolysate pretreated by microwave with power of 400 W showed the highest value, which increased by 7.58% compared to the control. The ratio of total hydrophobic amino acids to the TAA of MP hydrolysate showed the most increased amplitude with the microwave power of 300 W. The antioxidant activity of MP hydrolysate was related to the peptide content, and it was also relevant to the amino acid category and content. In conclusion, microwave pretreatment is an effective method for the preparation of antioxidant peptides and an increase in antioxidant stability.
Effect of Different Watering Regimes on Olive Oil Quality and Composition of Coratina Cultivar Olives Grown on Karst Soil in Croatia
Authors: Brkić Bubola Karolina , Kolega Šimun , Marcelić Šime , Šikić Zoran , Gašparović Pinto Ana , Zorica Marko , Klisović Dora , Novoselić Anja , Jukić Špika Maja , Kos Tomislav
Published: June 2022

Croatian islands are olive growing areas characterized by poor conditions for olive trees because of karst soil and a precipitation deficiency. Under these conditions, irrigation is a very important factor for constant olive oil production. This paper aims to investigate the effects of different watering regimes on quantity, sensory and chemical quality and composition of Coratina cv. olive oil obtained from trees grown on ameliorate karst soil during two harvesting years. Olive trees were subjected to rainfed conditions and three different irrigation treatments (T1—deficit irrigation representing the usual producer’s practice, T2—regulated deficit irrigation in respect to phenological stages, T3—full irrigation). Irrigation treatments increased oil yield compared to rainfed conditions (T1 + 58%, T2 + 66% and T3 + 74%, representing average values for both studied years). All olive oil samples were of extra quality. Irrigation led to a decrease in carotenoids, volatiles, polyunsaturated fatty acids and linolenic acid contents, with no difference found among irrigation treatments. Total phenols and secoiridoids concentration was not affected by irrigation, indicating that similar oil quality could be achieved with less demand on the water supply. Obtained results could help producers to define a suitable irrigation management in particular conditions of ameliorate karst.
How Can We Help Healthcare Workers during a Catastrophic Event Such as the COVID-19 Pandemic?
Authors: Wozniak Hannah , Benzakour Lamyae , Larpin Christophe , Sgardello Sebastian , Moullec Grégory , Corbaz Sandrine , Roos Pauline , Vieux Laure , Juvet Typhaine M. , Suard Jean-Claude , Weissbrodt Rafaël , Pugin Jérôme , Pralong Jacques A. , Cereghetti Sara
Published: June 2022

Healthcare workers (HCWs) have significantly suffered during the COVID-19 pandemic, reporting a high prevalence of anxiety, depression and post-traumatic stress disorder (PTSD). We investigated with this survey whether HCWs benefitted from supportive measures put in place by hospitals and how these measures were perceived. This cross-sectional survey, which was conducted during the first wave of COVID-19 at the Geneva University Hospitals, Switzerland, between May and July 2021, collected information on the use and perception of practical and mental health support measures provided by the hospital. In total, 3461 HCWs participated in the study. Regarding the practical support measures, 2896 (84%) participants found them useful, and 2650 (76%) used them. Regarding the mental health support measures, 3149 (90%) participants found useful to have the possibility of attending hypnosis sessions, 3163 (91%) to have a psychologist within hospital units, 3202 (93%) to have a medical nursing psychiatric permanence available seven days a week, and 3171 (92%) to have a hotline available seven days a week. In total, 436 (13%) HCWs used at least one of the available mental health support measures. During the COVID-19 pandemic, the support measures were valued by HCWs. Given the high prevalence of psychiatric issues among HCWs, these measures seem necessary and are likely to have alleviated the suffering of HCWs.
Polycystic Ovary Syndrome (PCOS) and Non-Suicidal Self-Injury (NSSI): A Community-Based Study
Authors: Williams Sophie , Fido Dean , Sheffield David
Published: June 2022

Polycystic Ovary Syndrome (PCOS) is an endocrine condition that has been associated with atypical emotional regulation strategy use as well as elevated levels of depression, anxiety, self-harm and suicidal ideation. Despite the existence of clinical screening guidance for this population, there is still little to no understanding of how non-suicidal self-injury and suicidal ideation and intention manifest in women with PCOS and how this might differ from women without PCOS. Within this cross-sectional investigation, women with and without a diagnosis of PCOS (n = 418) completed validated metrics of emotion dysregulation, rumination and non-suicidal self-injury (NSSI), as well as self-reported indices of previous suicidal ideation and future suicidal intention. Group comparisons indicated that women with, relative to those without, PCOS reported significantly greater metrics across all variables. Moreover, serial mediation analyses were conducted to test the ideation-to-action framework of suicide in women with PCOS, with the positive relationship between a PCOS diagnosis and future suicidal intention being explained through the indirect pathway of increased emotion dysregulation, recent suicidal ideation and NSSI. Our findings call to action the need for international screening for suicide intention and self-harm in women with PCOS.
The Impact of the Green Finance Reform and Innovation Pilot Zone on the Green Innovation—Evidence from China
Authors: Zhang Yanbo , Li Xiang
Published: June 2022

This article uses the “Green Finance Reform and Innovation Pilot Zone” promulgated in 2017 as an example to construct a quasi-natural experiment and uses the difference-in-difference method to test the impact of the implementation of the “Green Finance Reform and Innovation Pilot Zone” on the green innovation activities. It was found that the policy promotes the quantity and quality of corporate green innovation. The mechanism test showed that policy promotes the R&D investment and expands the credit scale. The study further found that green finance policies enhance the green innovation of enterprises as government environmental regulation is strengthened. Finally, green innovation by state-owned enterprises is more strongly promoted in the pilot green finance reform and innovation zones, and green innovation by enterprises in non-polluting sectors is more sensitive to the policy, with a heterogeneous pattern of policy effects in eastern and non-eastern China. Therefore, green finance policies should be promoted to achieve an effective combination of financial resource allocation and corporate green innovation to promote the construction of ecological civilization.
Effects of a Social and Emotional Competence Enhancement Program for Adolescents Who Bully: A Quasi-Experimental Design
Authors: Song Yul-mai , Kim Sunah
Published: June 2022

Background: The purpose of this study was to develop a social and emotional competence enhancement (SECE) program as an intervention for adolescents who bully, and to investigate its effects on school bullying behavior and mental health. Methods: A pre-posttest, 1-month follow-up nonequivalent control group quasi-experimental design was used. In total, 71 school bullies were included in the analysis. Results: The effects of this program were significant with regard to group-by-time interaction effects on social competence, emotional regulation, empathy, and school bullying behavior at the 1-month follow-up. Conclusions: The results indicate that the SECE program was effective at reducing school bullying behavior in adolescents who bully. School and community-based mental health professionals can provide feasible interventions that can be used in the short term to reduce school bullying behavior in adolescents who bully.
Enablers and Barriers of Accessing Health Care Services among Older Adults in South-East Asia: A Scoping Review
Authors: Mohd Rosnu Nurul Syuhada , Singh Devinder Kaur Ajit , Mat Ludin Arimi Fitri , Ishak Wan Syafira , Abd Rahman Mohd Harimi , Shahar Suzana
Published: June 2022

South-East Asia (SEA) is the home of the largest number of the world’s older population. In this scoping review, we aimed to map the existing enablers and barriers of accessing healthcare services among older adults in SEA countries. Articles that were published from January 2001 until November 2021 were searched in four data sources (PubMed, Web of Science, EBSCO Host and The Cochrane Library). Studies pertaining to the factors which assist or obstruct older Southeast Asian adults from assessing healthcare services were chosen for this scoping review. First, two reviewers screened the titles and abstracts of articles in the data sources. After identifying appropriate articles, the reviewers read them. Data extracted by one reviewer were verified by the other reviewer. The findings were then classified according to Penchansky and Thomas’s five domains of access. A total of 19 studies were included in the final scoping review. Accessibility and acceptability were the two factors most often identified as enablers or barriers to older adults from accessing healthcare. Other often mentioned factors were finances, transportation and social/family support. Older adults living in rural areas were especially impacted by these factors. To promote healthy ageing, optimum healthcare and wellbeing among older adults in Southeast Asia, it is extremely important to consider accessibility and acceptability when planning healthcare services.
Employment Status and Alcohol-Attributable Mortality Risk—A Systematic Review and Meta-Analysis
Authors: Saul Celine , Lange Shannon , Probst Charlotte
Published: June 2022

Being unemployed has been linked to various health burdens. In particular, there appears to be an association between unemployment and alcohol-attributable deaths. However, risk estimates presented in a previous review were based on only two studies. Thus, we estimated updated sex-stratified alcohol-attributable mortality risks for unemployed compared with employed individuals. A systematic literature search was conducted in August 2020 using the following databases: Embase, MEDLINE, PsycINFO, and Web of Science. The relative risk (RR) of dying from an alcohol-attributable cause of death for unemployed compared with employed individuals was summarized using sex-stratified random-effects DerSimonian-Laird meta-analyses. A total of 10 studies were identified, comprising about 14.4 million women and 19.0 million men, among whom there were about 3147 and 17,815 alcohol-attributable deaths, respectively. The pooled RRs were 3.64 (95% confidence interval (CI): 2.04–6.66) and 4.93 (95% CI 3.45–7.05) for women and men, respectively. The findings of our quantitative synthesis provide evidence that being unemployed is associated with an over three-fold higher risk of alcohol-attributable mortality compared with being employed. Consequently, a global public health strategy connecting brief interventions and specialized care with social services assisting those currently unemployed is needed.
Quantification of Recruit Training Demands and Subjective Wellbeing during Basic Military Training
Authors: Bulmer Sean , Drain Jace R. , Tait Jamie L. , Corrigan Sean L. , Gastin Paul B. , Aisbett Brad , Rantalainen Timo , Main Luana C.
Published: June 2022

Purpose: Assess and describe the physical demands and changes in subjective wellbeing of recruits completing the 12 week Australian Army Basic Military Training (BMT) course. Methods: Thirty-five recruits (24.8 ± 6.8 y; 177.4 ± 10.1 cm, 75.6 ± 14.7 kg) consented to daily activity monitoring and weekly measures of subjective wellbeing (Multi-component Training Distress Scale, MTDS). The physical demands of training were assessed via wrist worn activity monitors (Actigraph GT9X accelerometer). Physical fitness changes were assessed by push-ups, sit-ups and multi-stage shuttle run in weeks 2 and 8. Results: All objective and subjective measures significantly changed (p < 0.05) across the 12 week BMT course. In parallel, there was a significant improvement in measures of physical fitness from weeks 2 to 8 (p < 0.001). The greatest disturbance to subjective wellbeing occurred during week 10, which was a period of field training. Weeks 6 and 12 provided opportunities for recovery as reflected by improved wellbeing. Conclusions: The physical demands of training varied across the Australian Army 12 week BMT course and reflected the intended periodization of workload and recovery. Physical fitness improved from week 2 to 8, indicating a positive training response to BMT. Consistent with findings in sport, wellbeing measures were sensitive to fluctuations in training stress and appear to have utility for individual management of personnel in the military training environment.
Short-Term High-Intensity Circuit Training Does Not Modify Resting Heart Rate Variability in Adults during the COVID-19 Confinement
Authors: García-Suárez Patricia C. , Aburto-Corona Jorge A. , Rentería Iván , Gómez-Miranda Luis M. , Moncada-Jiménez José , Lira Fábio Santos , Antunes Barbara Moura , Jiménez-Maldonado Alberto
Published: June 2022

Background/Objective: The quarantine caused by the COVID-19 pandemic increased sedentary behavior, psychological stress, and sleep disturbances in the population favoring the installation of alterations in the cardiovascular system. In this sense, physical exercise has widely been suggested as an efficient treatment to improve health. The current study determined the impact of short-term high-intensity circuit training (HICT) on resting heart rate variability (HRV) in adults. Methods: Nine healthy participants (age: 31.9 ± 4.4 yr.) performed 36 HICT sessions (3 times per day; 3 days per week) and four participants (age: 29.5 ± 1.7 yr.) were assigned to a control group. The HICT consisted of 12 min of whole-body exercises performed during a workout. Twenty-four hours before and after the exercise program, HRV parameters were recorded. Results: The heart rate exercise during the last session trended to be lower when compared with the first HICT session (p = 0.07, d = 0.39, 95% CI = −13.50, 0.72). The interval training did not modify the HRV time (Mean NN, SDNN, RMSSD, NN50, pNN50) and frequency (LF, HF, LF/HF ratio, total power) domain parameters. Conclusion: Thirty-six HICT sessions did not provide enough stimuli to modify the resting HRV in adults during social isolation elicited by the COVID-19 pandemic. However, the data suggested that exercise protocol did not induce cardio-vagal adaptations.
Telephone-Administered Cognitive Behavioral Therapy for Body Dysmorphic Disorder: Case Series
Authors: Drüge Marie , Roth Tanja , Watzke Birgit
Published: June 2022

Cognitive behavioral therapy is an effective treatment for body dysmorphic disorder (BDD), but many patients do not receive appropriate treatment due to several treatment barriers and psychosocial care structures. Low-threshold interventions, including those from the field of e-mental health, could improve access to psychotherapy. In addition to internet-administered therapy, telephone-administered therapy may reduce treatment barriers, especially during the COVID-19 pandemic. This article presents four case reports of the same treatment (12 weeks of telephone-administered cognitive behavioral therapy accompanied by a workbook) applied to patients with body dysmorphic disorder during the summer of 2020. Three patients who completed the treatment had clinically relevant reductions in body dysmorphic and depressive symptoms and improved insight. One patient did not complete the telephone-administered therapy because her symptoms worsened, and she needed a more intensive form of treatment. These findings encourage future studies on the efficacy and effectiveness of telephone-administered treatment for BDD and its role in stepped-care models.
Mediation Analyses of the Role of Apathy on Motoric Cognitive Outcomes
Authors: Ceïde Mirnova E. , Eguchi Daniel , Ayers Emmeline I. , Lounsbury David W. , Verghese Joe
Published: June 2022

Recent literature indicates that apathy is associated with poor cognitive and functional outcomes in older adults, including motoric cognitive risk syndrome (MCR), a predementia syndrome. However, the underlying biological pathway is unknown. The objectives of this study were to (1) examine the cross-sectional associations between inflammatory cytokines (Interleukin 6 (IL-6) and C-Reactive Protein (CRP)) and apathy and (2) explore the direct and indirect relationships of apathy and motoric cognitive outcomes as it relates to important cognitive risk factors. N = 347 older adults (≥65 years old) enrolled in the Central Control of Mobility in Aging Study (CCMA). Linear and logic regression models showed that IL-6, but not CRP was significantly associated with apathy adjusted for age, gender, and years of education (β = 0.037, 95% CI: 0.002–0.072, p = 0.04). Apathy was associated with a slower gait velocity (β = −14.45, 95% CI: −24.89–4.01, p = 0.01). Mediation analyses demonstrated that IL-6 modestly mediates the relationship between apathy and gait velocity, while apathy mediated the relationships between dysphoria and multimorbidity and gait velocity. Overall, our findings indicate that apathy may be an early predictor of motoric cognitive decline. Inflammation plays a modest role, but the underlying biology of apathy warrants further investigation.
Abundance of Ixodes ricinus Ticks (Acari: Ixodidae) and the Diversity of Borrelia Species in Northeastern Poland
Authors: Kubiak Katarzyna , Szymańska Hanna , Dmitryjuk Małgorzata , Dzika Ewa
Published: June 2022

Monitoring the abundance of ticks and the prevalence of pathogens in ticks is an important activity in assessing the risk of tick-borne diseases and helps to develop preventive measures. This study aimed to estimate the density of Ixodes ricinus, the prevalence of Borrelia species, and their diversity in northeastern Poland. The overall mean I. ricinus density was 9.7 ticks/100 m2. There were no differences between years, subregions, or habitats of study. The Borrelia infection rate was higher in females (22.6%) and males (14.3%) than in nymphs 5.5% (MIR). The most infected ticks came from the eastern subregion (10.1%) where the incidence of borreliosis among the inhabitants was over 20% higher than in the other subregions. In the infected ticks, B. afzelii (38.3%) and B. garinii (34.5%) were predominant. B. bavariensis was confirmed in I. ricinus in Poland for the first time. The most polymorphic was B. garinii. B. miyamotoi (belonged to the European type) was identified as a mono-infection in 0.9% of ticks and in 1.5% as a co-infection with B. afzelii and with B. garinii. Besides the risk of borreliosis and co-infections with different Borrelia species, physicians should also be aware of B. miyamotoi infections among patients.
Genome-Wide Identification and Functional Analysis of the bZIP Transcription Factor Family in Rice Bakanae Disease Pathogen, Fusarium fujikuroi
Authors: Zhao Kehan , Liu Lianmeng , Huang Shiwen
Published: June 2022

Fungal basic leucine zipper (bZIP) proteins play a vital role in biological processes such as growth, biotic/abiotic stress responses, nutrient utilization, and invasion. In this study, genome-wide identification of bZIP genes in the fungus Fusarium fujikuroi, the pathogen of bakanae disease, was carried out. Forty-four genes encoding bZIP transcription factors (TFs) from the genome of F. fujikuroi (FfbZIP) were identified and functionally characterized. Structures, domains, and phylogenetic relationships of the sequences were analyzed by bioinformatic approaches. Based on the phylogenetic relationships with the FfbZIP proteins of eight other fungi, the bZIP genes can be divided into six groups (A–F). The additional conserved motifs have been identified and their possible functions were predicted. To analyze functions of the bZIP genes, 11 FfbZIPs were selected according to different motifs they contained and were knocked out by genetic recombination. Results of the characteristic studies revealed that these FfbZIPs were involved in oxygen stress, osmotic stress, cell wall selection pressure, cellulose utilization, cell wall penetration, and pathogenicity. In conclusion, this study enhanced understandings of the evolution and regulatory mechanism of the FfbZIPs in fungal growth, abiotic/biotic stress resistance, and pathogenicity, which could be the reference for other fungal bZIP studies.
Osteoclastogenesis and Osteogenesis
Authors: Kim Jung-Eun
Published: June 2022

Bone is a highly dynamic tissue that is continuously remodeled to attain and maintain optimal bone integrity, mass, and strength [...]
Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-Dystroglycan
Authors: Umezawa Fumiko , Natsume Makoto , Fukusada Shigeki , Nakajima Kazuki , Yamasaki Fumiya , Kawashima Hiroto , Kuo Chu-Wei , Khoo Kay-Hooi , Shimura Takaya , Yagi Hirokazu , Kato Koichi
Published: June 2022

The dystrophin–glycoprotein complex connects the cytoskeleton with base membrane components such as laminin through unique O-glycans displayed on α-dystroglycan (α-DG). Genetic impairment of elongation of these glycans causes congenital muscular dystrophies. We previously identified that glycerol phosphate (GroP) can cap the core part of the α-DG O-glycans and terminate their further elongation. This study examined the possible roles of the GroP modification in cancer malignancy, focusing on colorectal cancer. We found that the GroP modification critically depends on PCYT2, which serves as cytidine 5′-diphosphate-glycerol (CDP-Gro) synthase. Furthermore, we identified a significant positive correlation between cancer progression and GroP modification, which also correlated positively with PCYT2 expression. Moreover, we demonstrate that GroP modification promotes the migration of cancer cells. Based on these findings, we propose that the GroP modification by PCYT2 disrupts the glycan-mediated cell adhesion to the extracellular matrix and thereby enhances cancer metastasis. Thus, the present study suggests the possibility of novel approaches for cancer treatment by targeting the PCYT2-mediated GroP modification.
Involvement of Ceramide Signalling in Radiation-Induced Tumour Vascular Effects and Vascular-Targeted Therapy
Authors: Sharma Deepa , Czarnota Gregory J.
Published: June 2022

Sphingolipids are well-recognized critical components in several biological processes. Ceramides constitute a class of sphingolipid metabolites that are involved in important signal transduction pathways that play key roles in determining the fate of cells to survive or die. Ceramide accumulated in cells causes apoptosis; however, ceramide metabolized to sphingosine promotes cell survival and angiogenesis. Studies suggest that vascular-targeted therapies increase endothelial cell ceramide resulting in apoptosis that leads to tumour cure. Specifically, ultrasound-stimulated microbubbles (USMB) used as vascular disrupting agents can perturb endothelial cells, eliciting acid sphingomyelinase (ASMase) activation accompanied by ceramide release. This phenomenon results in endothelial cell death and vascular collapse and is synergistic with other antitumour treatments such as radiation. In contrast, blocking the generation of ceramide using multiple approaches, including the conversion of ceramide to sphingosine-1-phosphate (S1P), abrogates this process. The ceramide-based cell survival “rheostat” between these opposing signalling metabolites is essential in the mechanotransductive vascular targeting following USMB treatment. In this review, we aim to summarize the past and latest findings on ceramide-based vascular-targeted strategies, including novel mechanotransductive methodologies.
TRPM8 as an Anti–Tumoral Target in Prostate Cancer Growth and Metastasis Dissemination
Authors: Grolez Guillaume P. , Chinigò Giorgia , Barras Alexandre , Hammadi Mehdi , Noyer Lucile , Kondratska Kateryna , Bulk Etmar , Oullier Thibauld , Marionneau-Lambot Séverine , Le Mée Marilyne , Rétif Stéphanie , Lerondel Stéphanie , Bongiovanni Antonino , Genova Tullio , Roger Sébastien , Boukherroub Rabah , Schwab Albrecht , Fiorio Pla Alessandra , Gkika Dimitra
Published: June 2022

In the fight against prostate cancer (PCa), TRPM8 is one of the most promising clinical targets. Indeed, several studies have highlighted that TRPM8 involvement is key in PCa progression because of its impact on cell proliferation, viability, and migration. However, data from the literature are somewhat contradictory regarding the precise role of TRPM8 in prostatic carcinogenesis and are mostly based on in vitro studies. The purpose of this study was to clarify the role played by TRPM8 in PCa progression. We used a prostate orthotopic xenograft mouse model to show that TRPM8 overexpression dramatically limited tumor growth and metastasis dissemination in vivo. Mechanistically, our in vitro data revealed that TRPM8 inhibited tumor growth by affecting the cell proliferation and clonogenic properties of PCa cells. Moreover, TRPM8 impacted metastatic dissemination mainly by impairing cytoskeleton dynamics and focal adhesion formation through the inhibition of the Cdc42, Rac1, ERK, and FAK pathways. Lastly, we proved the in vivo efficiency of a new tool based on lipid nanocapsules containing WS12 in limiting the TRPM8–positive cells’ dissemination at metastatic sites. Our work strongly supports the protective role of TRPM8 on PCa progression, providing new insights into the potential application of TRPM8 as a therapeutic target in PCa treatment.
Protease and DNase Activities of a Very Stable High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix
Authors: Timofeeva Anna M. , Kostrikina Irina A. , Dmitrenok Pavel S. , Soboleva Svetlana E. , Nevinsky Georgy A.
Published: June 2022

Only some human organs, including the liver, are capable of very weak self-regeneration. Some marine echinoderms are very useful for studying the self-regeneration processes of organs and tissues. For example, sea cucumbers Eupentacta fraudatrix (holothurians) demonstrate complete restoration of all organs and the body within several weeks after their division into two parts. Therefore, these cucumbers are a prospective model for studying the general mechanisms of self-regeneration. However, there is no data available yet concerning biomolecules of holothurians, which can stimulate the processes of organ and whole-body regeneration. Investigation of these restoration mechanisms is very important for modern medicine and biology because it can help to understand which hormones, nucleic acids, proteins, enzymes, or complexes play an essential role in self-regeneration. It is possible that stable, polyfunctional, high-molecular-weight protein complexes play an essential role in these processes. It has recently been shown that sea cucumbers Eupentacta fraudatrix contain a very stable multiprotein complex of about 2000 kDa. The first analysis of possible enzymatic activities of a stable protein complex was carried out in this work, revealing that the complex possesses several protease and DNase activities. The complex metalloprotease is activated by several metal ions (Zn2+ > Mn2+ > Mg2+). The relative contribution of metalloproteases (~63.4%), serine-like protease (~30.5%), and thiol protease (~6.1%) to the total protease activity of the complex was estimated. Metal-independent proteases of the complex hydrolyze proteins at trypsin-specific sites (after Lys and Arg). The complex contains both metal-dependent and metal-independent DNases. Mg2+, Mn2+, and Co2+ ions were found to strongly increase the DNase activity of the complex.
Solvent-Free Preparation of Tannic Acid Carbon Dots for Selective Detection of Ni2+ in the Environment
Authors: Li Yan , Liu Can , Chen Menglin , An Yulong , Zheng Yunwu , Tian Hao , Shi Rui , He Xiahong , Lin Xu
Published: June 2022

Carbon dots (CDs) are widely used nanomaterials that not only exhibit good biocompatibility and photostability, but also benefit from a simple preparation process and easy functionalization, making them promising for broad applications in the fields of heavy metal ion detection and optoelectronic devices. Based on the excellent optical properties of CDs and the current situation of increasing energy shortages, this paper selects the natural polyphenolic compound tannic acid (TA) found in biomass materials as the carbon source and innovatively adopts a simple and convenient solvent-free pyrolysis method without auxiliary reagents or solvents. The CDs with good water solubility and certain fluorescence properties were directly prepared under the condition of high temperature, and the obtained CDs exhibited blue fluorescence, and a high QY of 35.4% was obtained at 300 °C. The analysis and results demonstrate the selectivity of these CDs for the detection of various metal ion solutions. In particular, these CDs are sensitive to Ni2+ and can be used as fluorescent sensors for the efficient and sustainable detection of Ni2+, whereas previous sensors were often specific to Fe3+ and Hg2+. Thus, a new sensing technique has been developed for the detection of Ni2+ to achieve more sensitive and rapid detection.
A Study of Type II ɛ-PL Degrading Enzyme (pldII) in Streptomyces albulus through the CRISPRi System
Authors: Li Qinyu , Chen Xiaojia , Wu Yuanjie , Chen Zheng , Han Yang , Zhou Peng , Shi Jiping , Zhao Zhijun
Published: June 2022

ε-Poly-L-lysine (ε-PL) is a widely used antibacterial peptide polymerized of 25–35 L-lysine residues. The antibacterial effect of ε-PL is closely related to the polymerization degree. However, the mechanism of ε-PL degradation in S. albulus remains unclear. This study utilized the integrative plasmid pSET152-based CRISPRi system to transcriptionally repress the ε-PL degrading enzyme (pldII). The expression of pldII is regulated by changing the recognition site of dCas9. Through the ε-PL bacteriostatic experiments of repression strains, it was found that the repression of pldII improves the antibacterial effect of the ε-PL product. The consecutive MALDI-TOF-MS results confirmed that the molecular weight distribution of the ε-PL was changed after repression. The repression strain S1 showed a particular peak with a polymerization degree of 44, and other repression strains also generated ε-PL with a polymerization degree of over 40. Furthermore, the homology modeling and substrate docking of pldII, a typical endo-type metallopeptidase, were performed to resolve the degradation mechanism of ε-PL in S. albulus. The hydrolysis of ε-PL within pldII, initiated from the N-terminus by two amino acid-binding residues, Thr194 and Glu281, led to varying levels of polymerization of ε-PL.