Ultra-Performance Liquid Chromatography-Quadrupole-Time-of-Flight-Mass Spectrometry-Based Analysis of Facial Physiological Parameters and Lipid Composition of Between Sensitive Skin of Women Aged 36–42 and 43–49 Year
Authors: Li Yu , Tang Rong , Yue Lizhi , He Congfen 
Published: January 2025

Background: UPLC-Q-TOF-MS (Ultra-Performance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry) is a high-precision, high-density technology for lipid analysis. Sensitive skin is a sub-stable condition, and it has been reported that the population of sensitive skin in China is predominantly female. Meanwhile, women with sensitive skin have different physiological parameters as well as lipid compositions at different ages. The Yellow Emperor’s Classic of Internal Medicine states that the number of women’s life cycles is seven, that major changes are manifested every 7 years, and that aging begins at age 35. At present, the correlation between facial lipid composition and aging indicators of sensitive skin in women aged 36–42 and 43–49 years has not been reported. Objective: This study reveals the relationship between key lipid composition of the facial skin and the aging of sensitive skin in women aged 36–42 and 43–49 years. Methods: We used UPLC-Q-TOF-MS technology to study the changes in lipid composition in the sensitive skin of woman aged 36–42 and 43–49 years, using a multi-probe adapter system with different types of skin-testing probes to test physiological parameters. Three types of multivariate data—questionnaires, physiological indicators, and lipid composition—were used together to assess differences in aging in a population of women with sensitive skin at different ages. Results: 1. In the questionnaire part, the T1 group was more susceptible to sunburn and the T2 group was more susceptible to tanning. 2. In the physiological index part, the aging characteristics of facial skin in the T2 group were obvious, with the b-value, as well as the brown area size, being significantly higher than the T1 group, while the TWEL, sebum, R2 value, ITA value, pore count, and concentration of the red area were significantly lower than the T1 group. 3. In the lipid part, the total facial lipid content was higher in the T2 group, with a significantly higher GP lipid, and the 47 VIP lipids obtained were analyzed by ROC curves, narrowing down to six lipids, PS(2-OMe-21:0/0:0), PS(O-18:0/20:5 (5Z,8Z,11Z,14Z,17Z)), PA(O-16:0/20:5 (5Z,8Z,11Z,14Z,17Z)), PS (P-16:0/12:0, PA (O-16:0/22:2 (13Z,16Z)), and PC (19:3 (10Z,13Z,16Z)/0:0)), and all six lipids were higher in the T2 group. 4. In Spearman correlation analysis, PS(O-18:0/20:5(5Z,8Z,11Z,14Z,17Z)), PS(P-16:0/12:0), PS(2-OMe-21:0/0:0), PA(O-16:0/20:5(5Z,8Z,11Z,14Z,17Z)), and PC(19:3( 10Z,13Z,16Z)/0:0), which are five lipids and skin aging indicators (TWEL, sebum, ITA value, b-value, pore count, concentration of red area, and brown area size) were significantly correlated. Conclusions: Through correlation analysis, it was found that changes in the composition of skin surface lipids (SSLs) in both age groups have an important influence on facial physiological indicators (aging manifestations) and played an important role in furthering the understanding of sensitive skin aging. Therefore, these lipid components also provide theoretical support for the development of cosmetic ingredients that slow down the aging of sensitive skin.