bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel and Alternative Therapeutic Strategies for Controlling Avian Viral Infectious Diseases: Focus on Infectious Bronchitis and Avian Influenza
Authors: Abbas Ghulam , Yu Jia , Li Guangxing
Published: July 2022

The growth of poultry farming has enabled higher spread of infectious diseases and their pathogens among different kinds of birds, such as avian infectious bronchitis virus (IBV) and avian influenza virus (AIV). IBV and AIV are a potential source of poultry mortality and economic losses. Furthermore, some pathogens have the ability to cause zoonotic diseases and impart human health problems. Antiviral treatments that are used often lead to virus resistance along with the problems of side effects, recurrence, and latency of viruses. Though target hosts are being vaccinated, the constant emergence and re-emergence of strains of these viruses cause disease outbreaks. The pharmaceutical industry is gradually focusing on plant extracts to develop novel herbal drugs to have proper antiviral capabilities. Natural therapeutic agents developed from herbs, essential oils (EO), and distillation processes deliver a rich source of amalgams to discover and produce new antiviral drugs. The mechanisms involved have elaborated how these natural therapeutics agents play a major role during virus entry and replication in the host and cause inhibition of viral pathogenesis. Nanotechnology is one of the advanced techniques that can be very useful in diagnosing and controlling infectious diseases in poultry. In general, this review covers the issue of the poultry industry situation, current infectious diseases, mainly IB and AI control measures and, in addition, the setup of novel therapeutics using plant extracts and the use of nanotechnology information that may help to control these diseases.
Dual Synthetic Jets Actuator and Its Applications—Part I: PIV Measurements and Comparison to Synthetic Jet Actuator
Authors: Luo Zhenbing , Zhao Zhijie , Deng Xiong , Wang Lin , Xia Zhixun
Published: July 2022

In order to understand the differences between dual synthetic jets (DSJs) and synthetic jets (SJs), particle image velocimetry (PIV) technology is used to capture the basic flow field characteristics of a dual synthetic jet actuator (DSJA) and a synthetic jet actuator (SJA), and then a careful comparison between them is implemented. The results indicate that a cycle of the DSJ is divided into two stages. In the near-field downstream, a pair of synthetic jets entrain fluid around them and interact with each other, making the flow field complex, and the time-periodic diaphragm dominates them. There is an unfavorable phenomenon of “self-support” between the two jets. In the far-field downstream, the two jets merge into a single, more stable SJ with a higher velocity and a double characteristic frequency. The DSJs have also shown good vectoring characteristics, with the vectoring deflection angle (VDA) changing from about −46° to 46°. The above results demonstrate that the DSJA may replace the traditional SJA in all kinds of applications and extend the applying area of the SJ to more active flow control systems, which cannot be qualified by traditional SJA.
Design and Experiment of a Garlic Orientation and Orderly Conveying Device Based on Machine Vision
Authors: Chen Jianneng , Yu Chennan , Yao Kun , Zhou Yun , Zhou Binsong
Published: July 2022

Aiming at the problem that the existing garlic-root-cutting equipment requires the orientation and orderly conveying of garlic, a garlic orientation and orderly conveying method based on machine vision and mechanical orientation mechanisms was proposed and a garlic orientation and orderly conveying device was designed. To realize garlic posture recognition, garlic and garlic roots were used as detection objects, the YOLOv5s objection detection model was used to establish a garlic feature detection model, and the model was trained. The garlic orientation and orderly conveying device was developed and a garlic posture recognition test and garlic orientation and orderly conveying test were completed. The test results show that the success rate of posture recognition is 98.67% and the average accuracy rate of the garlic deviation angle is 99.11%. When the conveying speed is 95 mm⋅s−1 and the rotating speed is 55 rpm, the orientation success rate is 95.6% and the conveying efficiency reaches 75 garlic per minute, which meets the design requirements. The accuracy of the garlic posture recognition method and the rationality of the garlic orientation and orderly conveying device design are verified, and the automatic orientation and orderly conveying of garlic is realized.
Precision Agriculture Technologies for Crop and Livestock Production in the Czech Republic
Authors: Vrchota Jaroslav , Pech Martin , Švepešová Ivona
Published: July 2022

Modern technologies are penetrating all fields of human activity, including agriculture, where they significantly affect the quantity and quality of agricultural production. Precision agriculture can be characterised as an effort to improve the results of practical farming, achieving higher profits by exploiting the existing spatial unevenness of soil properties. We aim to evaluate precision agriculture technologies’ practical use in agricultural enterprises in the Czech Republic. The research was based on a questionnaire survey in which 131 farms participated. We validated the hypothesis through a Chi-squared test on the frequency of occurrence of end-use technology. The results showed that precision farming technologies are used more in crop than livestock production. In particular, 58.02% of enterprises use intelligent weather stations, 89.31% use uncrewed vehicles, and 61.83% use navigation and optimisation systems for optimising journeys. These technologies are the most used and closely related to autonomous driving and robotics in agriculture. The results indicate how willing are agricultural enterprises to adopt new technologies. For policy makers, these findings show which precision farming technologies are already implemented. This can make it easier to direct funding towards grants and projects.
Sustainable Agriculture through Reduced Emission and Energy Efficiency: Estimation of Input–Output Energy and GHG Emission under Tunnel Cultivation of Tomato
Authors: Ali Qamar , Abbas Azhar , Khan Muhammad Tariq Iqbal , Bagadeem Salim , Alotaibi Bader Alhafi , Tariq Muhammad , Traore Abou
Published: July 2022

Tomato production under tunnel structures has shown promising returns in recent years in Pakistan. However, the energy use and GHGs dynamics remain largely unknown for tomato production under controlled conditions. This study estimates the energy budgeting and GHG emission in off-season (tunnel-farming) tomato production. Study data were gathered from 70 tunnel tomato growers through a simple random sampling technique. Energy use efficiency, energy productivity, and net energy along with covariates of energy output were estimated through Cob–Douglas regression. The results indicate that the total input energy consumption and production were 91,376.38 MJ ha−1 and 56,764.64 MJ ha−1, on average, respectively. The contribution of fertilizers (60.78%) was higher in total input energy followed by diesel and chemicals. The value of energy use efficiency was 0.652, which was higher for small farms (0.678) and lower (0.604) for large farms. Energy productivity (0.815 kg MJ−1), specific energy (1.355 MJ Kg−1), and net energy (−34,611.743 MJ ha−1) were also estimated. The total greenhouse gas emission was 3426.66 kg CO2 eq. ha−1, which is low for large farms (3197.57 kg CO2 eq. ha−1). The contribution of farmyard manure to total GHG emissions was high. The results show the inefficient use of inputs, responsible for GHG emissions. Fertilizers were a major contributor both in total input energy and GHG emission. The efficient utilization of agricultural inputs is a solution to reduce GHGs emissions in crop production. Therefore, the agriculture department should play its role to ensure the optimal or efficient use of agricultural inputs. The Department of Extension is working to guide farmers about crop production at each stage. Thus, regular visits from extension staff are recommended to guide vegetable producers about efficient input use.
Mobile Plant Disease Classifier, Trained with a Small Number of Images by the End User
Authors: Petrellis Nikos , Antonopoulos Christos , Keramidas Georgios , Voros Nikolaos
Published: July 2022

Mobile applications that can be used for the training and classification of plant diseases are described in this paper. Professional agronomists can select the species and their diseases that are supported by the developed tool and follow an automatic training procedure using a small number of indicative photographs. The employed classification method is based on features that represent distinct aspects of the sick plant such as, for example, the color level distribution in the regions of interest. These features are extracted from photographs that display a plant part such as a leaf or a fruit. Multiple reference ranges are determined for each feature during training. When a new photograph is analyzed, its feature values are compared with the reference ranges, and different grades are assigned depending on whether a feature value falls within a range or not. The new photograph is classified as the disease with the highest grade. Ten tomato diseases are used as a case study, and the applications are trained with 40–100 segmented and normalized photographs for each disease. An accuracy between 93.4% and 96.1% is experimentally measured in this case. An additional dataset of pear disease photographs that are not segmented or normalized is also tested with an average accuracy of 95%.
Age Evolution of Lipid Accretion Rate in Boars Selected for Lean Meat and Duroc Barrows
Authors: Sarri Laura , Balcells Joaquim , Seradj Ahmad Reza , Pena Ramona N. , Ramírez Gustavo A. , Tor Marc , de la Fuente Gabriel
Published: July 2022

Fatty acid (FA) deposition in growing–fattening pigs is mainly based on endogenous lipid synthesis, but also direct FA incorporation from the diet. To evaluate the direct fat incorporation rates and the endogenous desaturation action of the stearoyl-CoA desaturase (SCD) enzyme, a deuterium (D)-labeled saturated FA (d35-C18:0) was added to the diet. Sixteen three-way (3W) crossbred boars, and thirty-two purebred Duroc barrows homozygous for the SCD single nucleotide polymorphism rs80912566 (16 CC/16 TT), were used. Half of the animals of each genotype belonged to the growing and fattening phases. The fractional incorporation rate (FIR) of dietary fat in growing pigs was generally higher in adipose tissues, whereas in fattening pigs it was higher in the liver. Duroc pigs exhibited lower FIRs than 3W pigs, suggesting lower rates of endogenous synthesis by 3W pigs. Real fractional unsaturation rates (FURs) increased with age by the higher FIRs in 3W pigs and the de novo synthesis pathway in Duroc genotypes. Moreover, pigs carrying the SCD_T allele showed more enhanced oleic acid biosynthesis than Duroc CC pigs. In conclusion, suitable feeding protocols should be designed for each pig type to optimize production traits, considering that the metabolic pathway of FA for its deposition may differ.
Quantification of Canine Apocrine Gland Anal Sac Adenocarcinoma (AGASACA) Tumor Specimen Shrinkage after Formalin Fixation
Authors: Wustefeld-Janssens Brandan G. , Vinayak Arathi , Parker Lindsay A. , Hollenbeck Danielle L.
Published: July 2022

The aim was to prospectively measure the shrinkage of primary apocrine gland anal sac adenocarcinoma (AGASACA) tumors after 24 and 48 h of formalin fixation. Dogs that were diagnosed with AGASACA pre-operatively by aspiration cytology were prospectively enrolled in the study. Tumor extirpation was performed in a closed technique. The tumor and associated tissues were examined on the back table away from the patient and the widest dimension of the tumor was measured using a sterile ruler (Medline®; Northfield, IL, USA). This measurement was recorded in mm (t0). The tissue was placed in 10% buffered formalin and stored at room temperature. Two further measurements were taken after 24 (t24) and 48 (t48) hours of formalin fixation. Once the 48 h measurement was taken, the tissue was submitted for histopathology. The percentage of shrinkage between time points was calculated by using the following equation: (1 − [time b/time a]) × 100. Overall, 23 dogs with 23 tumors were enrolled. The mean percentage of shrinkage after 24 and 48 h of formalin fixation was 4.8% and 7.2%, respectively. The median diameter of the tumors reduced by 1 mm over 48 h and was not significantly different at any time point. These data will aid clinicians in interpreting measurements of AGASACA tumors following formalin fixation and shows that minimal change in tumor size is expected following 48 h.
The Uterus as an Influencing Factor for Late Embryo/Early Fetal Loss—A Clinical Update
Authors: Szelényi Zoltán , Kovács Levente , Szenci Ottó , Lopez-Gatius Fernando
Published: July 2022

Here we revise circumstances of non-infectious causes in which the uterus may be associated with pregnancy loss during the late embryo/early fetal period (following a positive pregnancy diagnosis in lactating dairy cows). As the uterine size increases with parity and pregnant heifers with no detrimental effects of a previous parturition, a primigravid uterus is proposed as a reference for identifying risk factors that negatively influence pregnancy in lactating cows. Cows suffering placenta retention or with a large uterus at insemination were selected as topics for this revision. Retained placenta, that occurs around parturition, has a long-lasting influence on subsequent pregnancy loss. Although retained placenta is a particularly predisposing factor for uterine infection, farm conditions along with cow factors of non-infectious cause and their interactions have been identified as main factors favoring this disorder. A large uterus (cervix and uterine horns lying outside the pelvic cavity) with no detectable abnormalities has been associated with low fertility and with a greater incidence of pregnancy loss. A large reproductive tract may well derive from an inadequate uterine involution. Therefore, peripartum management and strategies to reduce the incidence of uterine disorders should reduce their associated financial losses in the herds.
Experimental Study on Primary Bird Co-Infection with Two Plasmodium relictum Lineages—pSGS1 and pGRW11
Authors: Palinauskas Vaidas , Žiegytė Rita , Šengaut Jakov , Bernotienė Rasa
Published: July 2022

Background: Co-infections are common in the wild. Thus, studies focused on parasite interactions are essential. We aimed to (i) follow the development of two genetic lineages of Plasmodium relictum—pSGS1 and pGRW11—during single infections and co-infections and (ii) evaluate their impact on bird host health. Materials: Twenty-four domestic canaries were allocated to four groups: two groups were infected with parasites of a single genetic lineage, one group was infected with parasites of both genetic lineages, and one group was considered as the control group. Parasitemia, the number of polychromatophils, changes in body weight, and hemoglobin levels were all quantified up to 32 days post-infection. Results: Three birds infected with pSGS1 died within 20 days post-infection. The prepatent period and the peak of parasitemia did not differ significantly between groups. Differences in hemoglobin levels between the control and experimental groups were observed and there was an abnormal increase in the number of polychromatophils in infected birds. In all infected groups, correlations were detected between the number of polychromatophils and parasitemia (positive), and between the number of polychromatophils and hemoglobin levels (negative). Conclusion: This study shows that co-infection with two phylogenetically closely related P. relictum parasites does not alter overall parasitemia and does not cause higher virulence to the host.
Ultra-Processed Food Consumption during Pregnancy and Its Association with Maternal Oxidative Stress Markers
Authors: Rodríguez-Cano Ameyalli M. , González-Ludlow Isabel , Suárez-Rico Blanca V. , Montoya-Estrada Araceli , Piña-Ramírez Omar , Parra-Hernández Sandra B. , Reyes-Muñoz Enrique , Estrada-Gutierrez Guadalupe , Calzada-Mendoza Claudia C. , Perichart-Perera Otilia
Published: July 2022

Ultra-processed food (UPF) consumption during gestation may lead to increased oxidative stress (OS) and could affect pregnancy outcomes. This study aims to evaluate the association of UPF consumption during pregnancy with circulating levels of OS markers. Diet was assessed (average of three assessments) in 119 pregnant women enrolled in the OBESO perinatal cohort (Mexico), obtaining quantitative data and the percentage of energy that UPFs (NOVA) contributed to the total diet. Sociodemographic, clinical (pregestational body-mass index and gestational weight gain) and lifestyle data were collected. Maternal circulating levels of OS markers (malondialdehyde (MDA), protein carbonylation (PC), and total antioxidant capacity (TAC)) were determined at the third trimester of pregnancy. Adjusted linear regression models were performed to analyze the association between UPFs and OS markers. UPFs represented 27.99% of the total energy intake. Women with a lower UPF consumption (<75 percentile°) presented a higher intake of fiber, ω-3, ω-6, and a lower ω-6/3 ratio. Linear regression models showed that UPFs were inversely associated with TAC and MDA. Fiber intake was associated with PC. UPF intake during pregnancy may result in an increase in oxidative stress. When providing nutrition care, limiting or avoiding UPFs may be an intervention strategy that could promote a better antioxidant capacity in the body.
Inhibition of Cell Proliferation and Cell Viability by Sinecatechins in Cutaneous SCC Cells Is Related to an Imbalance of ROS and Loss of Mitochondrial Membrane Potential
Authors: Zhu Jiaqi , Gillissen Bernd , Dang Tran Dieu Linh , May Stefanie , Ulrich Claas , Stockfleth Eggert , Eberle Jürgen
Published: July 2022

The term sinecatechins designates an extract containing a high percentage of catechins obtained from green tea, which is commercially registered as Veregen or Polyphenon E (PE) and may be considered for treatment of cutaneous squamous cell carcinoma (cSCC) and actinic keratosis (AK). As shown here, treatment of four cSCC cell lines with 200 µg/mL of PE resulted in strong, dose-dependent decrease in cell proliferation (20–30%) as well as strongly decreased cell viability (4–21% of controls, 48 h). Effects correlated with loss of mitochondrial membrane potential, whereas early apoptosis was less pronounced. At the protein level, some activation of caspase-3 and enhanced expression of the CDK inhibitor p21 were found. Loss of MMP and induced cell death were, however, largely independent of caspases and of the proapoptotic Bcl-2 proteins Bax and Bak, suggesting that sinecatechins induce also non-apoptotic, alternative cell death pathways, in addition to apoptosis. Reactive oxygen species (ROS) were downregulated in response to PE at 4 h, followed by an increase at 24 h. The contributory role of initially reduced ROS was supported by the antioxidant N-acetyl cysteine, which in combination with PE further enhanced the negative effects on cell viability. Thus, sinecatechins inhibited cell proliferation and viability of cSCC cells, which could suggest the use of PE for AK treatment. The mechanisms appear as linked to an imbalance of ROS levels.
Effects of Coenzyme Q10 Supplementation on Oxidative Stress Markers, Inflammatory Markers, Lymphocyte Subpopulations, and Clinical Status in Dogs with Myxomatous Mitral Valve Disease
Authors: Druzhaeva Natalia , Nemec Svete Alenka , Tavčar-Kalcher Gabrijela , Babič Janja , Ihan Alojz , Pohar Katka , Krapež Uroš , Domanjko Petrič Aleksandra
Published: July 2022

Scarce data exist on the effects of coenzyme Q10 (CoQ10) supplementation in dogs with myxomatous mitral valve disease (MMVD). The purpose of this study was to investigate the effect of CoQ10 supplementation on oxidative stress markers (glutathione peroxidase, F2-isoprostanes), markers of inflammation (tumor necrosis factor-α, TNF soluble receptor II, leucocytes, and their subtypes), lymphocyte subpopulations (T helper and cytotoxic T lymphocytes, including activated T lymphocytes, and B lymphocytes), and echocardiographic and clinical parameters in dogs with MMVD. In this randomized, controlled, double-blind, longitudinal study, 43 MMVD dogs in stages ACVIM (American College of Veterinary Internal Medicine classification) B2 and ACVIM C and D (congestive heart failure (CHF)) received water-soluble coenzyme Q10 (100 mg twice daily) or placebo for 3 months, and 12 non-supplemented healthy dogs served as controls. All parameters were measured before and after supplementation in MMVD dogs and once in healthy dogs. CoQ10 supplementation had a positive impact on neutrophil percentage, lymphocyte percentage, and lymphocyte concentration in our cohort of dogs with CHF (ACVIM C and D). Conclusion: CoQ10 as an oral supplement may have benefits in terms of decreasing inflammation in dogs with MMVD and CHF.
Analysis of Score-Level Fusion Rules for Deepfake Detection
Authors: Concas Sara , La Cava Simone Maurizio , Orrù Giulia , Cuccu Carlo , Gao Jie , Feng Xiaoyi , Marcialis Gian Luca , Roli Fabio
Published: July 2022

Deepfake detection is of fundamental importance to preserve the reliability of multimedia communications. Modern deepfake detection systems are often specialized on one or more types of manipulation but are not able to generalize. On the other hand, when properly designed, ensemble learning and fusion techniques can reduce this issue. In this paper, we exploit the complementarity of different individual classifiers and evaluate which fusion rules are best suited to increase the generalization capacity of modern deepfake detection systems. We also give some insights to designers for selecting the most appropriate approach.
Screening of Potential Additives for Alleviating Slagging and Fouling during MSW Incineration: Thermodynamic Analysis and Experimental Evaluation
Authors: Chen Gang , Kang Shunshun , Zhao Kun , Zheng Anqing , Zhao Zengli
Published: July 2022

The formation of slagging and fouling during municipal solid waste (MSW) incineration not only significantly affects heat transfer, but also results in shortened operating cycles. In order to solve the issues, the effect of different additives on the migration and transformation patterns of alkali/alkaline earth metals (AAEM) and chlorine during MSW incineration is screened based on the Gibbs energy minimization method. The effect of potential additives on the ash fusion temperature and combustion reactivity of MSW char is subsequently verified and evaluated by experimental methods. The thermodynamic equilibrium analysis shows that Al(NO3)3, Ca(NO3)2, and Mg(NO3)2 have great potential to increase the ash fusion temperature. The experimental investigation confirms that the addition of Al(NO3)3, Ca(NO3)2, and Mg(NO3)2 significantly increases the ash fusion temperature. The order of increasing the ash fusion temperature by different additives is Mg(NO3)2 > Ca(NO3)2 > Al(NO3)3. The addition of Mg(NO3)2 significantly increased the initial deformation temperature, softening temperature, hemispheric temperature, and flow temperature of ash from 1180, 1190, 1200, and 1240 °C to 1220, 1230, 1240, and 1260 °C, respectively. The addition of Cu(NO3)2, Fe(NO3)3, and KMnO4 significantly decreases the temperature at the maximum weight loss rate of MSW char, while increasing the maximum weight loss rate. Additionally, Cu(NO3)2 shows the best performance in improving the combustion reactivity of MSW char. The addition of Cu(NO3)2 evidently increases the maximum weight loss rate from 0.49 to 0.54% °C−1. Therefore, it is concluded that Mg(NO3)2 and Cu(NO3)2 are supposed to be the most potential candidates for efficient additives. This study presents an efficient and economical method to screen potential additives for alleviating slagging and fouling during MSW incineration.
Primary Exploration of Leisure Path Design along Songhua River by a Small Number of Sample Experiment, Considering Several Multiple Indexes
Authors: Xi Tianyu , Zhang Xinyue , Jin Wenxin , Xu Weiqing , Wu Yu , Qin Huan
Published: July 2022

The waterfront park in northern China always has two parallel leisure pathways, a sunshine pathway and a tree-shaded pathway, which is attributed to the seasonal variations in water level. To provide some design suggestions according to the local characteristics of the waterfront park in northern China, this study selected six young volunteers to take part in an outdoor field experiment and a questionnaire survey in Stalin Park of Songhua River in Harbin, China. During the experiment, the volunteers’ local skin temperature and core temperature were recorded, with their subjective responses recorded every 5 min, including thermal comfort, thermal sensation, thermal pleasure, and fatigue scale vote. This study found that, compared with the sunshine pathway, the tree-shaded pathway not only optimized people’s outdoor thermal comfort and thermal pleasure, but also improved their fatigue scale vote experience. Some evidence showed that people’s subjective response to the outdoor thermal environment might be influenced by physical factors (temperature, velocity, humidity, radiation, etc.) and may also be influenced by the surrounding landscape view (water, square, lawn, tree, etc.). The first piece of evidence is that, during the first 10 min, people’s thermal sensation in the sunshine pathway group was high, but they kept voting for high thermal comfort, which may be due to the influence of the waterfront view on people’s subjective response to thermal comfort. The second piece of evidence shows that people’s overall thermal sensation was calculated by their local thermal sensation, looking at former research, with the voting results very different to the calculated results, which could be attributed to the influence of diversity landscape elements on people’s subjective response to thermal sensation. Based on these results, some suggestions for the leisure pathway design along Stalin Park of Songhua River in Harbin, China, were given. The shaded device of the sunshine pathway should be designed in 15-min-walk intervals and accessible ways to the tree-shaded pathway should be added. The other facilities should be designed with 30 min walking distance on the tree-shaded pathway and 20 min walking distance to the sunshine pathway. Diversified landscapes should be designed for both the tree-shaded pathway and sunshine pathway, which could improve people’s outdoor thermal comfort and the general subjective response to the environment. It is worth noting that the sample size of this study was small (6), and the participants were all homogenous young people (age, height, weight); thus, this study could be considered a preliminary work and the results and applicability have limitations.
Restrictive Atrial Dysfunction in Cardiac Amyloidosis: Differences between Immunoglobulin Light Chain and Transthyretin Cardiac Amyloidosis Patients
Authors: Versteylen Mathijs O. , Brons Maaike , Teske Arco J. , Oerlemans Marish I. F. J.
Published: July 2022

Background: In cardiac amyloidosis, the prevalence of thromboembolic events and atrial fibrillation is higher in transthyretin amyloidosis compared to immunoglobulin light chain amyloidosis. Therefore, we hypothesize that transthyretin cardiac amyloidosis patients have worse atrial function. Purpose: To explore the left atrial function by conventional ultrasound and strain analysis in immunoglobulin light chain- and transthyretin cardiac amyloidosis patients. Methods: In cardiac amyloidosis patients in our Amyloidosis Expert Center, echocardiographic strain analysis was performed using speckle tracking. Results: The data of 53 cardiac amyloidosis patients (83% male, mean age 70 years) were analyzed. Transthyretin cardiac amyloidosis patients (n = 24, 45%) were older (75 ± 5.6 vs. 65 ± 7.2 years, p < 0.001) and had more left ventricular (LV) hypertrophy than immunoglobulin light chain cardiac amyloidosis patients (n = 29, 55%). However, LV systolic and diastolic function did not differ, nor did left atrial dimensions (LAVI 56(24) vs. 50(31) mL/m2). Left atrial reservoir strain was markedly lower in transthyretin cardiac amyloidosis (7.4(6.2) vs. 13.6(14.7), p = 0.017). This association was independent of other measurements of the left atrial and ventricular function. Conclusions: Transthyretin cardiac amyloidosis patients had lower left atrial reservoir function compared to immunoglobulin light chain cardiac amyloidosis patients although the left atrial geometry was similar. Interestingly, this association was independent of left atrial- and LV ejection fraction and global longitudinal strain. Further research is warranted to assess the impact of impaired left atrial dysfunction in transthyretin cardiac amyloidosis on atrial fibrillation burden and prognosis.
Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli
Authors: Szpakowski Piotr , Ksiazek-Winiarek Dominika , Turniak-Kusy Malgorzata , Pacan Ilona , Glabinski Andrzej
Published: July 2022

For a long time, astrocytes were considered a passive brain cell population. However, recently, many studies have shown that their role in the central nervous system (CNS) is more active. Previously, it was stated that there are two main functional phenotypes of astrocytes. However, nowadays, it is clear that there is rather a broad spectrum of these phenotypes. The major goal of this study was to evaluate the production of some inflammatory chemokines and neurotrophic factors by primary human astrocytes after pro- or anti-inflammatory stimulation. We observed that only astrocytes induced by inflammatory mediators TNFα/IL-1a/C1q produced CXCL10, CCL1, and CXCL13 chemokines. Unstimulated astrocytes and those cultured with anti-inflammatory cytokines (IL-4, IL-10, or TGF-β1) did not produce these chemokines. Interestingly, astrocytes cultured in proinflammatory conditions significantly decreased the release of neurotrophic factor PDGF-A, as compared to unstimulated astrocytes. However, in response to anti-inflammatory cytokine TGF-β1, astrocytes significantly increased PDGF-A production compared to the medium alone. The production of another studied neurotrophic factor BDNF was not influenced by pro- or anti-inflammatory stimulation. The secretory response was accompanied by changes in HLA-DR, CD83, and GFAP expression. Our study confirms that astrocytes differentially respond to pro- and anti-inflammatory stimuli, especially to inflammatory cytokines TNF-α, IL-1a, and C1q, suggesting their role in leukocyte recruitment.
Proteomic Assessment of C57BL/6 Hippocampi after Non-Selective Pharmacological Inhibition of Nitric Oxide Synthase Activity: Implications of Seizure-like Neuronal Hyperexcitability Followed by Tauopathy
Authors: Hendrickx Jhana O. , Adams Charlotte , Sieben Anne , Laukens Kris , Van Dam Debby , De Meyer Guido R. Y.
Published: July 2022

Nitric oxide (NO) is a small gaseous signaling molecule responsible for maintaining homeostasis in a myriad of tissues and molecular pathways in neurology and the cardiovasculature. In recent years, there has been increasing interest in the potential interaction between arterial stiffness (AS), an independent cardiovascular risk factor, and neurodegenerative syndromes given increasingly epidemiological study reports. For this reason, we previously investigated the mechanistic convergence between AS and neurodegeneration via the progressive non-selective inhibition of all nitric oxide synthase (NOS) isoforms with N(G)-nitro-L-arginine methyl ester (L-NAME) in C57BL/6 mice. Our previous results showed progressively increased AS in vivo and impaired visuospatial learning and memory in L-NAME-treated C57BL/6 mice. In the current study, we sought to further investigate the progressive molecular signatures in hippocampal tissue via LC–MS/MS proteomic analysis. Our data implicate mitochondrial dysfunction due to progressive L-NAME treatment. Two weeks of L-NAME treatment implicates altered G-protein-coupled-receptor signaling in the nerve synapse and associated presence of seizures and altered emotional behavior. Furthermore, molecular signatures implicate the cerebral presence of seizure-related hyperexcitability after short-term (8 weeks) treatment followed by ribosomal dysfunction and tauopathy after long-term (16 weeks) treatment.
SARS-CoV-2, COVID-19, and Reproduction: Effects on Fertility, Pregnancy, and Neonatal Life
Authors: Harb Julien , Debs Nour , Rima Mohamad , Wu Yingliang , Cao Zhijian , Kovacic Hervé , Fajloun Ziad , Sabatier Jean-Marc
Published: July 2022

Since its discovery in Wuhan, China, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread over the world, having a huge impact on people’s lives and health. The respiratory system is often targeted in people with the coronavirus disease 2019 (COVID-19). The virus can also infect many organs and tissues in the body, including the reproductive system. The consequences of the SARS-CoV-2 infection on fertility and pregnancy in hosts are poorly documented. Available data on other coronaviruses, such as severe acute respiratory syndrome (SARS-CoV) and Middle Eastern Respiratory Syndrome (MERS-CoV) coronaviruses, identified pregnant women as a vulnerable group with increased pregnancy-related complications. COVID-19 was also shown to impact pregnancy, which can be seen in either the mother or the fetus. Pregnant women more likely require COVID-19 intensive care treatment than non-pregnant women, and they are susceptible to giving birth prematurely and having their newborns admitted to the neonatal intensive care unit. Angiotensin converting enzyme 2 (ACE2), a key player of the ubiquitous renin-angiotensin system (RAS), is the principal host cellular receptor for SARS-CoV-2 spike protein. ACE2 is involved in the regulation of both male and female reproductive systems, suggesting that SARS-CoV-2 infection and associated RAS dysfunction could affect reproduction. Herein, we review the current knowledge about COVID-19 consequences on male and female fertility, pregnant women, and their fetuses. Furthermore, we describe the effects of COVID-19 vaccination on reproduction.
A Study on the Sound Absorption Properties of Mycelium-Based Composites Cultivated on Waste Paper-Based Substrates
Authors: Walter Natalie , Gürsoy Benay
Published: July 2022

Mycelium-based composites have the potential to replace petrochemical-based materials within architectural systems and can propose biodegradable alternatives to synthetic sound absorbing materials. Sound absorbing materials help improve acoustic comfort, which in turn benefit our health and productivity. Mycelium-based composites are novel materials that result when mycelium, the vegetative root of fungi, is grown on agricultural plant-based residues. This research presents a material study that explores how substrate variants and fabrication methods affect the sound absorption properties of mycelium-based composites grown on paper-based waste substrate materials. Samples were grown using Pleurotus ostreatus fungi species on waste cardboard, paper, and newsprint substrates of varying processing techniques. Measurements of the normal-incidence sound absorption coefficient were presented and analyzed. This paper outlines two consecutive acoustic tests: the first round of experimentation gathered broad comparative data, useful for selecting materials for sound absorption purposes. The second acoustic test built on the results of the first, collecting more specific performance data and assessing material variability. The results of this study display that cardboard-based mycelium materials perform well acoustically and structurally and could successfully be used in acoustic panels.
Surface Plasmon Resonance Biosensors with Magnetic Sandwich Hybrids for Signal Amplification
Authors: Sun Ting , Li Mengyao , Zhao Feng , Liu Lin
Published: July 2022

The conventional signal amplification strategies for surface plasmon resonance (SPR) biosensors involve the immobilization of receptors, the capture of target analytes and their recognition by signal reporters. Such strategies work at the expense of simplicity, rapidity and real-time measurement of SPR biosensors. Herein, we proposed a one-step, real-time method for the design of SPR biosensors by integrating magnetic preconcentration and separation. The target analytes were captured by the receptor-modified magnetic nanoparticles (MNPs), and then the biotinylated recognition elements were attached to the analyte-bound MNPs to form a sandwich structure. The sandwich hybrids were directly delivered to the neutravidin-modified SPR fluidic channel. The MNPs hybrids were captured by the chip through the neutravidin–biotin interaction, resulting in an enhanced SPR signal. Two SPR biosensors have been constructed for the detection of target DNA and beta-amyloid peptides with high sensitivity and selectivity. This work, integrating the advantages of one-step, real-time detection, multiple signal amplification and magnetic preconcentration, should be valuable for the detection of small molecules and ultra-low concentrations of analytes.
Association between Individual Norepinephrine Transporter (NET) Availability and Response to Pharmacological Therapy in Adults with Attention-Deficit/Hyperactivity Disorder (ADHD)
Authors: Huang Jue , Mauche Nicole , Rullmann Michael , Ulke Christine , Becker Georg-Alexander , Patt Marianne , Zientek Franziska , Hesse Swen , Sabri Osama , Strauß Maria
Published: July 2022

Background: The role of the norepinephrine transporter (NET) has received increased focus in recent studies on the pathogenesis of attention-deficit/hyperactivity disorder (ADHD). The predictive value for pharmacological treatment and its link to other health or social limitations has been little-studied. This follow-up research on adult patients with ADHD aimed to explore whether the therapy response and health and social impairments depend on baseline individual NET availability. Methods: Data were collected from 10 patients on personal, family and professional situations, mental and physical health and treatments received after baseline via online and telephone surveys and were compared to baseline data to evaluate treatment-related changes. Results: The majority of our ADHD patients did not show therapy responses but showed improvements due to pharmacological treatment. There was no evidence of relationships between pre-treatment NET availability and therapy response or health/social limitations. Conclusions: Pharmacological monotherapy was insufficient to promote symptom remission, especially for participants with extreme insufficiency in NET availability, but improved outcomes in academic and social functioning. Psychotherapy should be considered as an add-on to the standard treatment approach due to its positive outcome in reducing social limitations. The prognostic value of individual NET availability in predicting the response to therapy needs further studies with large sample sizes.
The Schizotypal Ambivalence Scale: An Item Response Theory Analysis
Authors: Deters Lauren B. , Silvia Paul J. , Kwapil Thomas R.
Published: July 2022

Ambivalence has a prominent role in the historical formulations of schizotypy and schizophrenia, as well as borderline personality disorder. However, it has been overlooked by our current diagnostic nomenclature. The Schizotypal Ambivalence Scale (SAS) is a 19-item self-report scale developed to examine ambivalence relevant to schizotypy and schizophrenia-spectrum disorders. Questionnaire, interview, and ambulatory assessment studies support the construct validity of the measure as a predictor of schizophrenia-spectrum and borderline psychopathology. However, studies have not adequately examined the item properties and factor structure of the scale. To examine the psychometric features of the SAS, the present research applied item response theory and differential item functioning methods using a large sample of adults (n = 7096). Analyses of dimensionality were consistent with essential unidimensionality, and a 2PL IRT model found good item discrimination, an appropriate range of item difficulty, minimal local dependence, and excellent item fit. Analyses of differential item functioning found essentially no bias for gender on any items and very small effects for two items for racial/ethnic identity. Overall, the analyses reveal many psychometric strengths of the Schizotypal Ambivalence Scale and support its use a single-factor instrument for assessing ambivalence in diverse subgroups of adults.
An Empirical Analysis of Barriers to Building Information Modelling (BIM) Implementation in Wood Construction Projects: Evidence from the Swedish Context
Authors: Gharaibeh Lina , Matarneh Sandra , Eriksson Kristina , Lantz Björn
Published: July 2022

Building information modelling is gradually being recognised by the architecture, engineering, construction, and operation industry as a valuable opportunity to increase the efficiency of the built environment. Focusing on the wood construction industry, BIM is becoming a necessity; this is due to its high level of prefabrication and complex digital procedures using wood sawing machines and sophisticated cuttings. However, the full implementation of BIM is still far from reality. The main objective of this paper is to explore the barriers affecting BIM implementation in the Swedish construction industry. An extensive literature review was conducted to extract barriers hindering the implementation of BIM in the construction industry. Secondly, barriers to the implementation of BIM in the wood construction industry in Sweden were extracted using the grounded theory methodology to analyse expert input on the phenomenon of low BIM implementation in the wood construction industry in Sweden. Thirty-four barriers were identified. The analysis of this study also led to the development of a conceptual model that recommended solutions to overcome the barriers identified to help maximise BIM implementation within the wood construction industry. Identifying the main barriers affecting BIM implementation is essential to guide organisational decisions and drive policy, particularly for governments that are considering articulating regulations to expand BIM implementation.
Architectural Design Optimisation in Reticulated Free-Form Canopies
Authors: Stefańska Anna , Rokicki Wiesław
Published: July 2022

The search for the structural form of reticulated roofs is significant in interdisciplinary Architectural Design optimisation. Combining parametric design with structural logic influences the visual perception of the shape by choosing the most suitable technical solutions. Therefore, the divisions of reticulated structures should be determined to pursue structural, material and fabrication advancement. Structural divisions of free-formed canopies should simultaneously be solved in architectural and structural design at an early stage. Choosing a proper design becomes a complicated process, requiring the ability to select a type of production and rationalise technical solutions mainly due to the computer-aided design supported by algorithmic tools. Based on searching for optimal geometrical divisions, the case study investigates the differences between planar quadrilateral and triangular mesh panelisation. The study concludes the assets and flaws of both geometry shaping methods of reticulated structures based on minimal weight and fabrication aspects. The study concludes that implementing the manufacturing method of the chosen type of gridshells divisions into the architectural design optimisation enhances the resulting free-form structures at the early design stage.
The Role of PIM Kinases in Pediatric Solid Tumors
Authors: Julson Janet Rae , Marayati Raoud , Beierle Elizabeth Ann , Stafman Laura Lee
Published: July 2022

PIM kinases have been identified as potential therapeutic targets in several malignancies. Here, we provide an in-depth review of PIM kinases, including their structure, expression, activity, regulation, and role in pediatric carcinogenesis. Also included is a brief summary of the currently available pharmaceutical agents targeting PIM kinases and existing clinical trials.
Immune Checkpoint Inhibitors in Cancer Therapy—How to Overcome Drug Resistance?
Authors: Lao Yefang , Shen Daoming , Zhang Weili , He Rui , Jiang Min
Published: July 2022

Immune checkpoint inhibitors (ICIs), antagonists used to remove tumor suppression of immune cells, have been widely used in clinical settings. Their high antitumor effect makes them crucial for treating cancer after surgery, radiotherapy, chemotherapy, and targeted therapy. However, with the advent of ICIs and their use by a large number of patients, more clinical data have gradually shown that some cancer patients still have resistance to ICI treatment, which makes some patients unable to benefit from their antitumor effect. Therefore, it is vital to understand their antitumor and drug resistance mechanisms. In this review, we focused on the antitumor action sites and mechanisms of different types of ICIs. We then listed the main possible mechanisms of ICI resistance based on recent studies. Finally, we proposed current and future solutions for the resistance of ICIs, providing theoretical support for improving their clinical antitumor effect.
Insights into Metabolic Activity and Structure of the Retina through Multiphoton Fluorescence Lifetime Imaging Microscopy in Mice
Authors: Kesavamoorthy Niranjana , Junge Jason A. , Fraser Scott E. , Ameri Hossein
Published: July 2022

Fluorescence lifetime imaging microscopy (FLIM) evaluates the metabolic state of tissue based on reduced nicotinamide adenine dinucleotide (NAD(P)H) and flavin adenine dinucleotide (FAD). Fluorescence lifetime imaging ophthalmoscopy (FLIO) can image the fundus of the eyes, but cannot detect NAD(P)H. We used multiphoton FLIM to study the metabolic state of the retina in fixed eyes of wild-type mice C57BL6/J. We sectioned the eye using a polyacrylamide gel-embedding technique and estimated the percentage of bound NAD(P)H. We found that oxidative phosphorylation was the predominant metabolic state, particularly in the inner retina, when a fixed retina was used. We also demonstrated the feasibility of FAD imaging of the retina. In addition, we demonstrated that autofluorescence and various FLIM channels, such as hemoglobin, melanin and collagen, can be used to evaluate the structure of the retina and other parts of the eye without any special staining.