bluuebunny/crossref_metadata_2025 huggingface dataset:
Analiza porównawcza kluczowych rozwiązań w zakresie konsolidacji sprawozdań
finansowych zgodnie z MSSF oraz ustawą o rachunkowości
Authors: Gierusz Maciej
Published: September 2024

Cel: Rachunkowość finansowa, a szczególnie jej regulacje w zakresie konsolidacji,
są próbą wiernego i rzetelnego odzwierciedlenia skutków transakcji gospodarczych.
Podlegają więc ewolucji w miarę coraz lepszego rozumienia istoty ekonomicznej ujmowanych
zdarzeń. Głównym celem artykułu jest analiza porównawcza dwóch źródeł regulacji
skonsolidowanej sprawozdawczości finansowej, jakimi są MSR/MSSF oraz polskie prawo
bilansowe. Cele pomocnicze to prezentacja ewolucji zasad przygotowywania
skonsolidowanych sprawozdań finansowych według MSR/MSSF i ustawy o rachunkowości,
wskazanie przyczyn wprowadzania zmian oraz krytyczna ocena obowiązujących rozwiązań w
kontekście istoty ekonomicznej transakcji gospodarczych. Metodyka: Wykorzystane metody
badawcze obejmowały studia literaturowe, analizę aktów prawnych oraz skonsolidowanych
sprawozdań finansowych. Wyniki: Artykuł składa się z trzech części. Pierwsza stanowi
wprowadzenie, w którym nakreślono problematykę oraz przedstawiono rys historyczny
rozwoju regulacji w zakresie konsolidacji. W drugiej części przeanalizowane zostały
najważniejsze obszary różnic. Należą do nich: kontrola nad jednostką zależną, wywieranie
znaczącego wpływu oraz sprawowanie wspólnej kontroli, rozliczanie transakcji połączeń
gospodarczych metodą nabycia, wycena wartości firmy, ujmowanie udziałów należących do
właścicieli mniejszościowych oraz inne wybrane szczegółowe zagadnienia z analizowanego
obszaru. Zakres opracowania obejmuje kwestie zarówno koncepcyjne, jak i związane z
wyceną i prezentacją pozycji charakterystycznych dla skonsolidowanych sprawozdań
finansowych. Implikacje i rekomendacje: Ostatnią trzecią częścią jest podsumowanie
przeanalizowanych różnic między dwoma źródłami regulacji oraz próba wskazania, które z
nich jest bliższe zasadzie jasnego i rzetelnego obrazu oraz wiarygodnego ujmowania
skutków transakcji w sprawozdaniu finansowym. Oryginalność/wartość: Zdaniem autora
rozstrzygnięcia MSR/MSSF w zakresie konsolidacji sprawozdań finansowych należy ocenić
wyżej polskie prawo bilansowe z wyjątkiem amortyzacji wartości firmy.
Kształtowanie się dochodów gmin z tytułu opłaty miejscowej w czasie pandemii
COVID-19
Authors: Dziuba Jarosław , Życzkowska Elżbieta
Published: September 2024

Cel: Celem artykułu jest ukazanie kształtowania się dochodów gmin w Polsce z
tytułu opłaty miejscowej w czasie pandemii COVID-19 (lata 2020-2022) na tle okresów
przed pandemią (lata 2017-2019). Metodyka: W artykule zastosowano metodę analizy aktów
prawnych dotyczących przeciwdziałania rozprzestrzenianiu się pandemii i jej skutków oraz
dotyczących źródeł finansowania JST. Przeprowadzono statystyczną analizę wykorzystania
opłaty miejscowej przez gminy w Polsce wraz z analizą jej zależności od wybranych
czynników ekonomicznych. Wyniki: W czasie pandemii COVID-19 dochody gmin z tytułu opłaty
miejscowej uległy zmniejszeniu zarówno w wymiarze bezwzględnym, jak i w odniesieniu do
dochodów ogółem i własnych. Powodem tego było zmniejszenie się aktywności turystycznej
ludności wskutek wprowadzanych ograniczeń dotyczących funkcjonowania branży turystycznej
i przemieszczania się ludności. Spadek ten był krótkotrwały i nie wpłynął na ogólną
sytuację finansową JST ze względu na marginalny udział opłaty w strukturze dochodów.
Implikacje i rekomendacje: Przykłady nielicznych gmin wskazują na duży potencjał
dochodowy opłaty miejscowej, co implikuje konieczność dalszych badań nad tym źródłem
dochodów w celu zwiększenia jego powszechności i wydajności. Oryginalność/wartość: W
literaturze przedmiotu względnie niewiele jest opracowań poświęconych w całości
pojedynczym dochodom JST, szczególnie o marginalnej, w skali całego sektora, wydajności
dochodowej. Artykuł częściowo wypełnia tę lukę.
A Derelomine Weevil Severely Damaging the Inflorescence and Young Fruits of Date Palm
Authors: El-Shafie Hamadttu A.F. , Abdel-Banat Babikr M.A.
Published: September 2024

The inflorescence (spathe) weevil, Derelomus sp. has become a serious pest of date palm in Saudi Arabia in recent years. The weevil reduces date yield drastically by causing 20‐50% damage in fruit set and the losses may reach 100% if no proper management
measures are practiced. To date, no experimental data are available on the weevil’s biology, ecology, and management. This article highlights the taxonomy, distribution, bionomics, and possible management options of this important economic pest.
Cell-based in vitro hemoassay for evaluation of NLRP3-inflammasome activity
Authors: Ossina N. K. , Volova L. T. , Lebedev P. A. , Shafieva I. A. , Pugachev E. I. , Goncharenko S. A. , Kuznetsov S. I. , Gusyakova O. A. , Svetlova G. N.
Published: September 2024

Currently, gouty arthritis is considered as a polygenic multifactorial autoinflammatory disease caused by activation of the NOD (nucleotide-binding domain) -like protein receptor 3 inflammasome NLRP3. The two cytokines IL-1β and IL-18 are considered important biomarkers of NLRP3 inflammasome activation. However, usually the concentration of IL-1β in donor sera is extremely low and found to be at the limit of detection level (1-3 pg/ml), while the concentration of circulating cytokine IL-18 in the sera of individual donors varies greatly. This results in difficulty using these biomarkers in the diagnosis of autoinflammatory diseases. We hypothesized that the patient’s blood cells which were sensitized in vivo to the presence of specific factors characteristic of autoinflammatory diseases, in particular, gouty arthritis, would produce increased amounts of the inflammasome-regulated cytokines compared to blood cells obtained from healthy donors. A comparison of the IL-18 cytokine in healthy donors and patients with gouty arthritis was carried out using 2 methods: a) by traditional analysis of the level of serum circulated IL-18 and b) by using a cell-based Hemoassay in vitro developed at the research institute “Biotech” SamGMU. The comparative analysis demonstrated the advantages of using an in vitro cell-based Hemoassay to assess the IL-18 cytokine status of patients. Serum IL-18 values varied widely and showed no significant difference between donors and patients with gouty arthritis. Using the developed cell-based Hemoassay in vitro, significant quantitative differences in the production of the inflammatory cytokine IL-18 produced by blood cells of potentially healthy donors and patients with gouty arthritis were detected. Blood cells of individual patients, sensitized in vivo with specific factors characteristic of gouty arthritis, produce increased concentrations of IL-18 in the cell growth medium in vitro compared to cells from healthy donors. Thus, the in vitro cell-based Hemoassay can be used for a more accurate assessment of the cytokine status of patients.
Early markers of induced pluripotent stem cell hematopoietic development
Authors: Sheveleva O. N. , Nenasheva T. A. , Lyadova I. V.
Published: September 2024

The generation of immune cell populations from induced pluripotent stem cells (iPSCs) is a valuable model to study mechanisms that control hematopoietic development; it also is a promising approach to develop immunotherapeutic strategies to treat various diseases, including hereditary, oncological and infectious ones. To date, it has been demonstrated that iPSCs can differentiate into different immune cells, including macrophages, neutrophils, natural killer cells and T cells. However, the protocols suggested so far are experimental, and they require optimization, standardization and scaling. Solution to these tasks requires methods allowing to predict the efficacy of ongoing differentiation at early differentiation stages. Here, we evaluated whether iPSC hematopoietic/myeloid differentiation can be monitored by means of flow cytometry. Human iPSCs were differentiated into hematopoietic/myeloid cells using two protocols previously suggested for the generation of macrophages from iPSCs. The protocols differed by methods used to induce early and late stages of cell differentiation. At early differentiation stages, the protocols differed by approaches used to induce the generation of mesoderm and hemogenic endothelium, i.e., 2D differentiation in the presence of exogenous factors known to promote mesoderm and hemogenic endothelium development (“factor-dependent” protocol) or 3D differentiation in the absence of exogenous cytokines and growth factors (“spontaneous” protocol). At late differentiation stages, the protocols differed by factors added to the cultures to promote hematopoietic/myeloid specification (i.e., SCF, FGF2, IL-6, IL-3 and M-CSF or only IL-3 and M-CSF). At different stages of differentiation, the expressions of antigens known to be expressed by mesoderm, hemogenic endothelium and hematopoietic cells (i.e., CD309, CD34, CD31, CD43 and CD45) were evaluated. At early differentiation stages, the main phenotypic changes observed in cell cultures were an upregulation of the expression of CD309 (a receptor for vascular endothelial growth factor), the appearance of the expression of sialophorin CD43 and the expression of CD34 antigen. In cells cultured in 2D factor-dependent conditions, these changes appeared earlier and were more pronounced as compared with cells cultured in 3D “spontaneous” conditions. The results suggest that CD309 and/or CD43 are valuable markers for an early prediction of the effectiveness of iPSC differentiation into hematopoietic/ myeloid progeny.
Expression of CD14+CD282+, CD14+CD284+, CD14+HLA-DR+, CD14+CD11b+ receptors and sIgA level in premature infants with K. pneumoniae
Authors: Chistyakova G. N. , Remizova I. I. , Ustyuzhanin A. V. , Makhanyok A. A. , Kadochnikova P. A. , Abakarova D. A.
Published: September 2024

K. pneumoniae is one of the leading microorganisms causing nosocomial infections among premature newborns. The ineffectiveness of immune defense, morphofunctional immaturity, length of hospitalization and invasive procedures create the prerequisites for the implementation of the infectious process in a hospital setting. The question of the reasons for the development of infection, the etiological agent of which colonizes the intestines, remains open. Purpose of the study: to evaluate the expression of CD14+CD282+, CD14+CD284+, CD14+HLA-DR+, and CD14+CD11b+ receptors on blood monocytes and the level of sIgA in coprofiltrates in premature infants with intestinal colonization by K. pneumonia with different genetic profiles. We examined 11 children with the uge gene (group 1), 20 newborns with the uge + fim genes (group 2), and 12 children with the kfu + uge + fim genes. Microbiological examination of feces included identification and antibiotic sensitivity of microorganisms. Detection of the uge, fim and kfu genes in K. pneumoniae strains was carried out by PCR. The expression level of monocyte activation markers was determined by flow cytometry. Gestational age and anthropometric parameters did not differ between newborns. Children identified with the fim gene in combination with other genes were more often discharged home with K. pneumoniae than with the uge gene. In these children, a decrease in the level of expression of CD14+CD282+, CD14+CD284+, CD14+CD11b+, CD14+HLA-DR+ receptors at birth and upon reaching postconceptional age, and a low sIgA content in coprofiltrates during 24 days of life were recorded. Thus, a decrease in the expression of CD14+CD282+, CD14+CD284+, CD14+CD282+ and CD14+HLA-DR+ receptors by blood monocytes and insufficiency of sIgA production in the large intestine determine a long period of colonization of K. pneumoniae strains with the presence of the fim gene in combination with other genes (from 15 to 180 days), as well as the possibility of Klebsiella infection occurring in subsequent periods of the child’s life. Much more often, children with the combination of genes uge + fim and kfu + uge + fim were discharged from the hospital with a diagnosis of anemia; only in these groups of children was the development of bronchopulmonary dysplasia recorded.
Expression of immune checkpoint markers in peripheral blood lymphocytes of women with fibroadenoma of the breast
Authors: Kurmyshkina O. V. , Kulikovskaya T. M. , Volkova T. O.
Published: September 2024

Changes of the immune state in patients with fibroadenoma (FA) or other benign lesions of the breast, as well as the involvement of immune checkpoints in pathogenesis of these lesions, remain underexplored. The aim of this study was to compare the expression level of the key immune checkpoint markers CD279/PD-1, CD274/PD-L1, CD366/TIM3, and CD223/LAG3 in total circulating lymphocytes, T cell population as well as in its CD4 and CD8 subsets in peripheral blood from women with breast FA and healthy controls. Blood samples were taken from 12 women diagnosed with FA of the breast (aged 23-54 years, FA group) and 15 healthy women (aged 22-52 years, control group). Sample uptake was performed immediately before surgery, and samples were further analyzed by multicolor flow cytometry using monoclonal antibodies CD3-VioBlue, CD4/CD8-FITC, PD1-PE, PD-L1-PerCP-Cy.5.5, and TIM3/LAG3-APC. Each sample was incubated with 4 antibody combinations: CD3/CD4/PD1/PD-L1/TIM3, CD3/CD4/PD1/PD-L1/LAG3, CD3/CD8/PD1/PD-L1/TIM3, and CD3/CD8/PD1/PD-L1/LAG3. First, mono-expression of each of the 4 immune checkpoint markers was evaluated in the lymphocyte gate from both investigated groups. In FA samples, a significant increase in PD-L1 expression (assessed as percent of positive cells and fluorescence intensity change) was observed. Regarding expression of immune checkpoints in CD3+ T cells, along with significantly increased %PD-L1+, elevated numbers of PD1+T cells were detected. As for the differences in immune checkpoint expression changes between CD4+ and CD8+ T cell subsets, FA patient group demonstrated a more prominent increase in the amount of CD8+PD1+T cells relative to CD4 subset. The profiles of PD-L1 changes in CD4 and CD8 subpopulations were comparable showing, in both cases, a significant increase in FA sample group. We also analyzed changes in co-expression of any two immune checkpoint markers in CD4+ and CD8+ T cell subsets. The most noticeable was as increase in the prevalence of PD1+PD-L1+ phenotype in both T cell subpopulations from FA patients compared to the controls. With respect to co-expression of 3 checkpoint markers in CD4+ and CD8+ T cells, a significant increase in the percentage of PD1+PD-L1+TIM3+ cells among CD4 T helpers was found in FA. Thus, specific changes of T cell phenotype related to (co-)expression of immune checkpoint regulators may occur in systemic circulation of women with breast FA.
Evaluation of the pharmacokinetics of an experimental combined form of IFNα and IFNγ for intranasal use
Authors: Kravchenko E. V. , Bezrukova E. V. , Vorobeychikov E. V. , Skvortsov N. V.
Published: September 2024

Polypous rhinosinusitis is a chronic inflammatory disease of the nasal mucosa and paranasal sinuses. Recombinant interferon has antiproliferative activity and corrects the deficiency of endogenous regulatory molecules, which allows us to consider this class of immunotropic drugs as a promising component of conservative immunotherapy for polyposis rhinosinusitis. Purpose of the study: To select the optimal composition of the experimental composition of interferons and evaluate their pharmacokinetic parameters in laboratory animals.

For laboratory evaluation of the effectiveness of the auxiliary components of the proposed composition in creating a local effect, Wistar rats in the amount of 30 animals were selected, to which the proposed form of the drug (IFN1) was administered once intranasally. The control group included 30 animals that were alternately administered intranasally with a similar dose of IFNα2b and IFNγ dissolved in water for injection (IFN2). Quantitative determination of interferon concentration in blood samples was carried out using the enzyme immunoassay method. To assess the dependence of changes in concentrations in the blood of animals on the time elapsed after their administration, standard pharmacokinetic models were used. Next, the integral from the initial moment of time to infinity was determined, which corresponded to the area under the pharmacokinetic curve and made it possible to calculate a number of pharmacokinetic characteristics.

The following indicators were obtained: 1) Area under the pharmacokinetic curve (AUCt) ng/ml/min – IFN1 = 683.0; IFN2 = 1707.7. 2) Absorption constant (Kp) – IFN1 = 0.13096; IFN2 = 0.03836. 3) Suction constant (Kel) – IFN1 = 0.00177; IFN2 = 0.00317. 4) Clearance (Cl) ml/min – IFN1 = 129.35; IFN2 = 51.73.

Based on the differences in the values of pharmacokinetic parameters (AUCt) for the IFN1 and IFN2 preparations, it can be concluded that the use of a composition based on chitosan, succinic acid and DMSO leads to a pronounced retention of interferon in the nasal mucosa, which provides a pronounced local therapeutic effect and allows for fewer systemic adverse reactions. This composition is suitable for further clinical studies of the effectiveness of immunotherapy for polyposis rhinosinusitis.
Flow cytometric analysis of the interaction of monoclonal antibodies to various epitopes of the HSP70 molecule with intracellular and membrane-associated forms of this protein
Authors: Ovsyanikova O. V. , Shustova O. A. , Grechikhina M. V. , Sapozhnikov A. M.
Published: September 2024

Currently, a large amount of data has demonstrated that many types of tumor cells, in contrast to their non-transformed forms, are characterized by the translocation of intracellular heat shock proteins 70 kDa (HSP70) to the surface of the plasma membrane. This made it possible to classify HSP70 exposed on the cell surface as a tumor-associated antigen and was the basis for searching the opportunities for the practical use of this phenomenon in clinical oncology. A significant argument in favor of the prospects of such studies was the discovered ability of HSP70, present on the surface of target cells, to enhance the cytotoxic activity of NK cells. In this regard, the work of many research groups is devoted to developing approaches to increasing the level of membrane-associated HSP70 in tumor tissues. At the same time, given the presence of such proteins on the surface of many types of tumor cells, the use of monoclonal antibodies that interact with HSP70 molecules for antitumor therapy can be considered as one of the promising approaches. It is well known that antibody preparations that selectively interact with cancer cells can be used for targeted antitumor immunotherapy. Previously, we obtained a panel of six B cell hybridomas producing monoclonal antibodies to the inducible and constitutive forms of human HSP70, directed to various epitopes of this molecule. It is significant that three varieties of hybridomas produced antibodies with specificity for binding sites on the C-terminal domain of the HSP70 molecule, and the second trio of monoclonal antibodies were specific to epitopes on the N-terminal domain of HSP70, while almost all known commercial antibodies interact only with C-terminal fragments of HSP70. In this work, we conducted a comparative study of the binding of the obtained antibodies to these proteins localized in different types of cells, both in the intracellular space and on the cell surface.

The results obtained allow us to consider the identified varieties of monoclonal antibodies that most effectively recognize surface HSP70 as a promising basis for the creation of new drugs for antitumor immunotherapy.
Immunobiological efficacy of a new sodium polyprenyl phosphate based medicine for the treatment and prevention of experimenrtal metabolic syndrome
Authors: Pronin A. V. , Cheknev S. B. , Nikolaeva T. N. , Kozlov V. V. , Kozhevnikova T. N. , Vostrova E. I. , Grigorieva E. A. , Sosnovskaya O. Yu. , Sarycheva M. A. , Vostrov A. V. , Narovlyansky A. N. , Sanin A. V.
Published: September 2024

The aim of the research was to study the population composition of the splenic lymphoid cells, to assess the functional activity of lymphocytes as well as the state of the gastrointestinal tract microbiota in experimental modeling of metabolic syndrome (MS).

The studies were conducted using two experimental models of MS and hyperlipidemia (HL), based on prolonged drinking of animals with 20% aqueous fructose solution with added cholesterol and intraperitoneal administration of Poloxamer 407 to mice, respectively.

The results of the experiments indicate a change in the population composition of splenocytes (decrease in CD4+ and CD8+T cells, activation of CD4+CD25+FoxP3+Thed cells), accompanied by a decrease in T cell activity and increased proliferation of B lymphocytes, impaired production of IL-15 and IL-22, as well as lipid and carbohydrate metabolism (adiponectin, leptin, insulin), which serves as a prerequisite for the development of chronic inflammation, which is a pathogenetic sign of MS.

We found changes in the intestinal microbiota of mice characteristic of the manifestation of metabolic dysbiosis – an increase in the representation of Firmicutes bacteria (staphylococci, streptococci, enterococci) in the biomaterial, changes in the content of facultative (E. coli) and transient (Enterobacter) microflora.

In order to develop a new kind of medicine for therapy and prevention of HL and MS, we used a combination of sodium polyprenyl phosphate (PP) and beta-sitosterol (BSS), polyisoprenoid derivatives of plant origin.

More pronounced changes were found in the splenocyte population composition and activation parameters of Treg cells in HL modeling compared with the MS model. The introduction of PP and BSS has an immunocorrective effect during treatment.

The therapeutic effect of this drug, as well as the prevention of the MS symptoms, is accompanied by normalization of the microbiota state.

The data obtained indicate the prospects of using PP and BSS for the prevention and treatment of HL and MS in order to influence the leading links in the pathogenesis of metabolic disease.
Mast cells as indicators of foreign particle biocompatibility
Authors: Artashyan O. S. , Khramtsova Yu. S. , Vlasova A. A. , Tyumentseva N. V. , Yushkov B. G.
Published: September 2024

The key problem of introducing nanoparticles into clinical practice for diagnostic and therapeutic purposes is their safety. Connective tissue, an important component of which are mast cells, reacts actively in response to foreign particles. The reaction of mast cells can be an indicator of the biocompatibility of foreign particles. The study was conducted on male Wistar rats. Iron-carbon nanoparticles in the FeC modification stabilized in an aqueous medium using an auxiliary substance were used. Tissue examination (liver, lungs, heart, thymus, kidneys) was performed 1, 7 and 30 days after injection. After the injection of nanoparticles, the largest quantity of them is found in the liver and lungs, a smaller quantity is found in the heart, kidneys and thymus. The liver and lungs are the main organs of excretion of nanoparticles due to phagocytes. The accumulation of nanoparticles in the liver leads to the development of destructive processes and to the activation of compensatory and adaptive mechanisms in the form of cellular and intracellular regeneration of hepatocytes. In organs with a low content of nanoparticles, structural changes are poorly expressed. The mast cells of the studied organs react differently to the introduction of nanoparticles. The first to react are the mast cells of the liver by reducing the quantity and increasing degranulation. The population of lung mast cells reacts unidirectionally by sharply activating degranulation without changing the amount. An increase in the secretory activity of mast cells in the lungs and liver indicates the participation of mast cells in the regulation of particle elimination through intercellular signaling pathways of interaction with the system of phagocytic mononuclear cells. Heart mast cells are involved in maintaining the inflammatory process in the early period of the experiment, contribute to the return of myocardial parameters to homeostatic norm in the late period of the experiment. Mast cells can be considered as indicators of biocompatibility nanoparticles. The absence of an inflammatory process and the preservation of the structural and functional characteristics of the tissues where nanoparticles accumulate, as well as the reaction of mast cells in them, indicate the relative safety of the particles under study.
TIM-3 expression on monocyte-derived dendritic cells
Authors: Tyrinova T. V. , Leplina O. Yu. , Chernykh E. R.
Published: September 2024

The T cell immunoglobulin domain and mucin domain-containing molecule-3 (TIM-3), an inhibitory checkpoint receptor, has been identified as a crucial regulator of cellular immune responses. TIM-3 has been discovered as a receptor involved in the negative regulation of T cells. Recent studies have demonstrated that TIM-3 is expressed on innate immune cells, including dendritic cells (DCs), even at a higher level than T cells. In the tumor microenvironment, the majority of DCs have a monocytic origin. Models for studying such DCs in vitro are DC cultures generated from monocytes in the presence of growth factors. The present study aimed to investigate the expression of TIM-3 in IFNα-induced monocyte-derived DCs (IFN-DCs) and the impact of DC activation on TIM-3 expression. DCs were obtained by culturing the adherent fraction of mononuclear cells from healthy donors for 4 days in the presence of GM-CSF and IFNα, followed by LPS addition for 24 hours. Human double-stranded DNA (dsDNA, 5 μg/mL) was added as an activation stimulus to intact IFN-DCs at the stage of maturation, along with LPS. Expression of the membrane TIM-3 molecule was determined by flow cytometry, and the level of expression of TIM-3 mRNA – by real-time RT-PCR with reverse transcription. Intact donor IFN-DCs expressed the membrane TIM-3 molecule at a high level (more than 70% of cells). The addition of LPS as a maturation stimulus almost halved the expression of TIM-3 (pW < 0.05) without affecting the expression of HAVCR2/TIM-3 mRNA. Exogenous dsDNA (along with LPS) increased the expression of HAVCR2/TIM-3 mRNA by more than three times (pW = 0.05) with a decrease in the number of TIM-3+DCs (pW = 0.003). Our findings indicate the presence of mechanisms that support expression of this inhibitory checkpoint receptor under conditions of DC activation. Further studies of the regulation of TIM-3 expression by monocyte-derived dendritic cells will expand the understanding of the biological significance of inhibitory receptors on DCs from the point of view of the immune response, as well as, in the future, increase the effectiveness of current approaches in cancer immunotherapy using IFN-DCs and inhibitors of checkpoint molecules.
Dívida externa e alternativas de desenvolvimento na América Latina
Authors: FFRENCH-DAVIS RICARDO
Published: September 2024

RESUMO O documento propõe que a origem do problema da dívida externa enfrentada pelos países em desenvolvimento está no funcionamento dos mercados de capitais internacionais. Uma visão otimista equivocada sobre o comportamento do mercado financeiro na década de 70 pode se estender por muitos anos, já que as nações devedoras adaptaram suas estruturas econômicas a um grande volume de recursos externos; pelo contrário, espera-se uma escassez de fundos nos últimos anos desta década. Essas tendências marcarão profundamente o desempenho social e econômico da maioria dos países dessa região. Algumas das principais opções que essas nações serão obrigadas a enfrentar são analisadas neste texto. Além da necessidade imperiosa de lutar por uma mudança na forma como a dívida externa vem sendo renegociada, é preciso também reorganizar suas políticas econômicas externas. Isso ocorre pela intensificação da cooperação econômica entre as nações devedoras e pelo início de uma nova fase de substituição de importações agora coordenada por meio da expansão das importações. Aqui também são demonstrados alguns fatos responsáveis por uma melhor capacidade de lidar e ajustar diferentes países de acordo com as estratégias econômicas que adotaram no passado e com a situação política que cada país enfrenta.
O déficit público: um modelo simples
Authors: ARIDA PÉRSIO
Published: September 2024

RESUMO Um modelo simples é desenvolvido para lidar com a dimensão financeira do público e sua relação com o ajuste externo. São discutidos os efeitos de uma desvalorização cambial, de uma flexibilização da restrição externa e o papel da política fiscal sob um efeito-riqueza bastante intenso. Por fim, um comentário sobre os determinantes da taxa de juros e seus aspectos intertemporais.
PUNÇÃO ASPIRATIVA COM AGULHA FINA NO DIAGNÓSTICO DO CORYNEBACTERIUM PSEUDOTUBERCULOSIS NA LINFADENITE CASEOSA CAPRINA
Authors: Ribeiro M.G. , Dias Junior J.G. , Paes A.C. , Barbosa P.G. , Nardi Júnior G. , Listoni F.J.P.
Published: September 2024

RESUMO Procedeu-se a citologia aspirativa com agulha fina em onze caprinos com aumento de linfonodos submandibulares, parotídeos e pré-escapulares. Do material aspirado dos linfonodos foi possível o isolamento microbiológico e a identificação citológica do Corynebacterium pseudotuberculosis em sete animais. O presente estudo pretendeu investigar a utilização da citologia aspirativa com agulha fina no diagnóstico citológico e microbiológico da linfadenite caseosa caprina.
FREQÜÊNCIA DE AGENTES CAUSADORES DE ENTERITES EM LEITÕES LACTENTES PROVENIENTES DE SISTEMAS DE PRODUÇÃO DE SUÍNOS DO ESTADO DE SÃO PAULO
Authors: Calderaro F.F. , Baccaro M.R. , Moreno A.M. , Ferreira A.J.P. , Jerez A.J. , Pena H.J.F.
Published: September 2024

RESUMO Considerando-se a importância econômica das enterites em leitões lactentes o presente estudo teve como objetivo verificar a freqüência de agentes etiológicos bacterianos, virais e parasitários em fezes de leitões com diarréia em sistemas de produção de suínos do Estado de São Paulo. Foram examinados 174 leitões com idade entre 1 a 38 dias provenientes de 21 sistemas de produção. Amostras de fezes foram submetidas ao exame bacteriológico, parasitológico e virológico. Dos 174 animais examinados, 117 foram positivos para um ou mais agentes pesquisados. Observou-se 70 (40,2%) animais positivos para presença de E. coli ETEC, 55 (31,6%) para Isospora suis, 19 (10,9%) para rotavírus, 2 (1,2%) para o Cryptosporidium parvum e 57 (32,8 %) dos animais amostrados revelaram-se negativos para qualquer agente estudado. A associação mais freqüente entre os agentes pesquisados foi I. suis e E. coli ETEC, presente em 19 animais (10,9%). A freqüência da I. suis foi significativamente maior em leitões com idade entre 8 e 14 dias. Em relação aos 70 leitões onde diagnosticou-se a presença de E. coli ETEC, 25,7% apresentaram genótipo Sta+, 21,5%-STb+, 7,1%-LT+, 4,3%-STa+STb+, 34,3%-STb+LT+ e 7,1%-Sta+STb+LT+. As informações obtidas neste estudo visam contribuir para o conhecimento dos principais microrganismos causadores de enterites em leitões em cidades do Estado de São Paulo, auxiliando os profissionais da área na adoção de medidas preventivas e de controle dos agentes.
ADSORÇÃO DE IONS FERRO E FLOCULAÇÃO DE CÉLULAS DE SACCHAROMYCES CEREVISIAE DURANTE O PROCESSO ELETROLÍTICO
Authors: Epiphanio R. , Bidoia E. D. , Corso C. R.
Published: September 2024

RESUMO A floculação que ocorre em células de Saccharomyces cerevisiae através de adsorção de íons divalentes foi estudada utilizando o processo eletrolítico com eletrodos de ferro fundido. Os íons ferro liberados foram analisados em soluções com ausência e presença de S. cerevisiae em diferentes tempos de eletrólise. Observou-se que a concentração de íons ferro liberados em solução após eletrólises era em média 10 vezes maior nas suspensões de S. cerevisiae do que em soluções salinas isentas de leveduras.
EPIDEMIOLOGIA E CONTROLE DAS MICOBACTERIOSES EM SUÍNOS NO SUL DO BRASIL - ESTIMATIVA DO IMPACTO ECONÔMICO
Authors: Martins L.S. , Leão S.C. , Morés N. , Silva V.S. , Dutra V. , Pinheiro S.R. , Balian S. , Homem V.S.F. , Ferreira F. , Ferreira Neto J.S.
Published: September 2024

RESUMO Foi realizado um estudo em nove abatedouros de suínos da Região Sul do Brasil, responsáveis por cerca de 40% do total de abate na Região, com o objetivo de se estimar o impacto econômico das micobacterioses para os produtores. O período estudado foi de janeiro de 1997 a dezembro de 1999. Para tanto, foram preenchidas planilhas com informações mensais a respeito do número total de suínos abatidos e ocorrência de condenação por linfadenite granulomatosa na ocasião do abate; outra planilha forneceu dados sobre os descontos impostos aos produtores dependendo do destino da carcaça condenada e valor médio da bonificação recebida pela sua tipificação. O impacto econômico foi de 1,5 milhão de reais em 1997; 4,3 milhões em 1998 e 6,9 em 1999. Cada 0,1% de incremento na prevalência das condenações por linfadenite granulomatosa representou um prejuízo de 201 mil reais em 1997, 531 em 1998 e 834 em 1999. As implicações e razões destas constatações foram discutidas com vistas à estruturação de um programa de controle racional.
CARACTERIZAÇÃO E REGISTRO DE AULACASPIS ROSAE (BOUCHÉ, 1833) (HEMIPTERA, DIASPIDIDAE) EM RUBUS SP. (ROSACEAE), RIO GRANDE DO SUL, BRASIL1
Authors: Wolff V.R.S. , Teixeira I.
Published: September 2024

RESUMO A identificação de cochonilhas tem sido muito importante por serem potenciais pragas, principalmente as exóticas que geralmente estão desacompanhadas de seus inimigos naturais. Uma grande infestação de Aulacaspis rosae (Bouché, 1833) foi encontrada em São Luiz, 3° Distrito de Farroupilha, RS, em outubro/1999. A preparação e determinação do material foi realizada no Laboratório de Entomologia da Fundação Estadual de Pesquisa Agropecuária, FEPAGRO, Porto Alegre, RS. A prevenção através de controle quarentenário deve ser mais rigorosa na introdução de plantas exóticas.
DESEMPENHO DE AZOXYSTROBIN NO CONTROLE DA FERRUGEM DO CAFEEIRO SOB CONDIÇÕES DE CHUVA INDUZIDA
Authors: Töfoli J.G. , Oliveira S.H.F. , Domingues R.J. , Santos J.M.F. , Martins E.M.F.
Published: September 2024

RESUMO O presente trabalho teve por objetivo avaliar o efeito de chuva induzida sobre a eficiência, persistência e sistemicidade de azoxystrobin no controle da ferrugem em mudas de café. O experimento foi realizado em condições de casa de vegetação sendo utilizado o delineamento experimental inteiramente casualizado com 7 tratamentos e 12 repetições, no qual metade das plantas teve o solo coberto por uma lâmina de papel alumínio. Após 60 e 120 minutos da pulverização as plantas foram submetidas a 30 mm de chuva controlada durante 30 minutos e inoculadas com esporos de H. vastatrix. Azoxystrobin destacou-se no controle da ferrugem sendo sempre superior ao padrão oxicloreto de cobre em todos os parâmetros estudados. Diferenças significativas na severidade da doença entre as duas condições de solo, coberto e descoberto, foram observadas, para os tratamentos com azoxystrobin, sendo sempre a condição descoberto superior a coberto, indicando sua sistemicidade.
PRESENÇA DE MYZOCALLIS KURICOLA (MATSUMURA, 1917) (HEMIPTERA: APHIDOIDEA: DREPANOSIPHIDAE) NO BRASIL E PRIMEIRO REGISTRO DA ESPÉCIE NA REGIÃO NEOTROPICAL
Authors: Bergmann E.C. , Ilharco F.A. , Souza-Silva C.R.
Published: September 2024

RESUMO O trabalho registra pela primeira vez, para o Brasil e para a Região Neotropical, a presença do afídeo Myzocallis kuricola (Matsumura, 1917) (Hemiptera: Aphidoidea: Drepanosiphidae). Também é novo para Portugal Continental e Açores. A espécie é nativa do Japão, Coréia e Taiwan e o primeiro registro fora de sua área de origem foi feito por ILHARCO (1984) no arquipélago da Madeira, Portugal sobre Castanea crenata Siebold & Zuccarini (Fagaceae). No Brasil os afídeos foram coletados sobre C. sativa Miller, C. vesca Gaertner e Castanea sp. e em Portugal Continental e Açores, sobre C. sativa. Desconhece-se qualquer referência à espécie como causadora de danos.
ESTUDO COMPARATIVO DOS TESTES 2-MERCAPTOETANOL E REAÇÃO DE FIXAÇÃO DO COMPLEMENTO NO SORODIAGNÓSTICO DA BRUCELOSE BOVINA
Authors: Paulin L.M. , Prado G.E.S. , Federsoni I.S.P. , Teixeira A.C. , Castro V. , Genovez M.E.
Published: September 2024

RESUMO O presente estudo teve como objetivo comparar o teste do 2-Mercaptoetanol (2-ME) em relação à reação de fixação do complemento (RFC') no sorodiagnóstico da brucelose, ambos escolhidos como confirmatórios pelo Programa Nacional de Combate à Brucelose Bovina do Ministério da Agricultura, Pecuária e Abastecimento no Brasil. Para tanto, foram processadas 77 amostras de soro de fêmeas bovinas vacinadas, com idade igual ou superior a 24 meses (grupo A) e 74 amostras de soro de fêmeas de bovinos não vacinadas (grupo B), totalizando 151 animais pertencentes a diferentes tipos de exploração e procedentes de cinco estados brasileiros. Foram identificados, através da RFC', 40,2% de animais reatores e 35,1% para o grupo B. O teste estatístico utilizado para analisar os resultados foi o de MCNEMAR, com intervalo de confiança de 5%, não havendo diferença estatística significativa entre os mesmos. O teste KAPPA foi usado como medida de concordância entre as provas e o resultado foi de 91,9% para o grupo A e 88,5% para o grupo B. Baseados nos dados, calculou-se a sensibilidade, a especificidade e a concordância do 2-ME em relação à RFC', teste padrão para a doença. Observou-se sensibilidade do 2-ME de 96,8% e 100% e especificidade 95,6% e 91,7%. Conclui-se que o 2-me é um ótimo teste confirmatório no sorodiagnóstico da brucelose bovina, revelando, também neste trabalho, possuir boa sensibilidade.
APHIDS (HEMIPTERA: APHIDIDAE) AND THEIR PARASITOIDS (HYMENOPTERA) ON IXORA SPP. (RUBIACEAE) IN THE STATES OF BAHIA AND SÃO PAULO, BRAZIL - FORMAL RECORDS OF INTERACTIONS
Authors: Imenes S.D.L. , Bergmann E.C. , Peronti A.L.B.G. , Ide S. , Martins J.E.R.
Published: September 2024

ABSTRACT This paper reports, for the first time, the incidence of the aphids Aphis gossypii Glover, 1877 and Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae) on Ixora macrothyrsa Teijsm. & Binn. (Rubiaceae) in Brazil. The formal records of interaction among Aphis spiraecola Patch, 1914 with Ixora coccinea L. and Ixora coccinea L. var. compacta Hort. in Brazil, and the presence of Aphelinus gossypii Timberlake, 1923 (Hymenoptera: Aphelinidae), parasitoid of A. spiraecola in the state of São Paulo, are made. Observations on other parasitoids (Hymenoptera) infesting these aphids were also made. The paper includes a short morphological characterization of the aphids, parasitoids, a survey of the ornamental plant hosts in Brazil and suggestions for control.
AVALIAÇÃO DA CITOTOXICIDADE DE BIFLAVONÓIDES ISOLADOS DE OURATEA SPECTABILIS (OCHNACEAE) EM CÉLULAS DE CÓRNEA DE COELHO SIRC
Authors: Simoni I.C. , Felicio J.D. , Gonçalez E. , Rossi M.H.
Published: September 2024

RESUMO A citotoxicidade dos biflavonóides 6,6"- bigenkwanin (OSP-1), 7,7"- dimetoxi-agathisflavona (OSP-2) e tetradimethoxiflavona (OSP-1 Met) isolados de folhas de Ouratea spectabilis foram avaliadas em linhagem celular de córnea de coelho, SIRC pela determinação da medida espectofotométrica nas concentrações das substâncias que inibem a absorbância em 50% do controle. OSP-1, OSP-2 e OSP-1 Met apresentaram IC50 nas concentrações de 381,9 ± 22,29; 436,74 ± 22,05 e 359,7 ± 24,25 µg/mL, respectivamente. Estes compostos apresentam atividade inibidora para a aldose redutase, enzima relacionada com o início da formação da catarata em diabéticos.
OCCURRENCE OF CRANEOPSYLLA MINERVA MINERVA (ROTHSCHILD, 1903) (SIPHONAPTERA, STEPHANOCIRCIDAE) FROM DIDELPHIS ALBIVENTRIS IN THE STATE OF RIO GRANDE DO SUL, BRAZIL
Authors: Müller G. , Brum J.G.W. , Berne M.E.A. , Ribeiro P.B.
Published: September 2024

ABSTRACT The occurrence of Craneopsylla minerva minerva is reported for the first time in the State of Rio Grande do Sul, Brazil, parasiting Didelphis albiventris (opossum).
BRACHYMERIA PODAGRICA (FABRICIUS) (HYMENOPTERA: CHALCIDIDAE) AS PARASITOIDS OF SARCODEXIA LAMBENS (WIEDEMANN) (DIPTERA: SARCOPHAGIDAE) IN BRAZIL
Authors: Marchiori C.H. , Pereira L.A. , Silva Filho O.M. , Ribeiro L.C.S. , Borges V.R.
Published: September 2024

ABSTRACT The objective of the study is to report the first occurrence of the parasitoid Brachymeria podagrica on pupae of Sarcodexia lambens. Human feces was used as bait to collect the insects. In the study, 33 pupae of Sarcodexia lambens (Wiedemann) (Diptera: Sarcophagidae) were obtained, 3 of which yielded the parasitoid Brachymeria podagrica (Hymenoptera: Chalcididae).
CONTRIBUIÇÃO À BIBLIOGRAFIA SOBRE CANCRO CÍTRICO - RESUMO DOS TRABALHOS PUBLICADOS POR PESQUISADORES DO INSTITUTO BIOLÓGICO E EM COLABORAÇÃO COM PESQUISADORES DE OUTRAS INSTITUIÇÕES (1957 - 1999)
Authors: Namekata T. , Cerávolo L.C. , Montes S.M.N. , Ferrari J.T. , Nogueira E.M. de C.
Published: September 2024

RESUMO Esse trabalho reúne os resumos de 73 artigos publicados pelos pesquisadores do Instituto Biológico e de outras instituições colaboradoras referentes ao cancro cítrico (Xanthomonas axonopodis pv. citri) desde a sua primeira constatação no Brasil, em 1957 até 1999, quando o Instituto Biológico era responsável pela pesquisa desta doença. Esse trabalho abrange estudos da etiologia, epidemiologia, métodos de diagnóstico e métodos de controle (erradicação entre outros).
QUANDO A FICÇÃO INVADE A PROSA: PRÁTICAS DISCURSIVAS NÃO-CANÔNICAS NO DISCURSO ACADÊMICO
Authors: Balocco Anna Elizabeth
Published: September 2024

Resumo Neste artigo, focalizam-se duas dissertações de mestrado não-canônicas e discutem-se as condições de possibilidade para sua emergência. O argumento apresentado desenvolve-se em duas direções: 1) as dissertações escapam à regra do “comentário” (FOUCAULT, 1996), mas não emergem exclusivamente como práticas sociais que rejeitam e, portanto, são parcialmente governadas por aquele princípio - ou pela dimensão do simbólico onde este princípio está codificado; e 2) no caso das dissertações analisadas, reconhecem-se diferentes dimensões discursivas no espaço simbólico onde se inserem: uma dimensão de certa cultura local, em determinada instituição; uma outra dimensão, relacionada à cultura de dada disciplina; uma dimensão ainda mais abrangente, relacionada a paradigmas epistemológicos. No final do trabalho, são feitos comentários sobre as relações entre o simbólico, como o local da mediação discursiva, e a prática social, o local de emergência do sujeito (LACLAU, 2000).
Properties of concrete with processed granulated blast-furnace slag as fine aggregate
Authors: Venkataramu Vibha , Reddy B V Venkatarama
Published: September 2024

Exploring the use of non-organic solid wastes from mines and mineral industries, such as metallurgical slags, and tailings as a replacement to natural sand in concrete can provide sustainable options to save the natural river sand reserves. River sand for construction is environmentally and economically unviable in several countries of the world. The present investigation intends to explore the suitability of unused granulated blast-furnace slag (GBS) stocks as fine aggregate in concrete. The GBS was processed using a vertical shaft impactor to modify the shape parameters of the granules. The processed GBS (PGBS) content in concrete was varied between 0 and 100%, and the mechanical and microstructural properties of concrete were characterised. The workability of fresh concrete, compressive strength, tensile strength, stress–strain characteristics, drying shrinkage and development of bond between concrete and reinforcement were investigated. The results show a considerable increase (∼40%) in the long-term strength of concrete with 100% PGBS. The PGBS-based concrete showed higher compressive strength than the strength of the reference concrete. Using X-ray diffraction and thermogravimetric analysis techniques, the hydration products present in the PGBS and river sand-based concrete were qualitatively and quantitatively assessed.
Co-expression of miRNA players in advanced laryngeal carcinoma – Insights into the roles of miR-93-5p, miR-145-5p, and miR-210-3p
Authors: Kyurkchiyan Silva , Petkova Veronika , Stancheva Gergana , Stancheva Iglika , Dimitrov Stoyan , Dobriyanova Venera , Popova Diana , Kaneva Radka , Popov Todor Miroslavov
Published: September 2024

Advanced laryngeal squamous cell carcinoma (LSCC) is the second most prevalent type of head and neck squamous cell carcinoma (HNSCC). Identifying microRNAs (miRNAs) related to key regulatory molecules or mechanisms could offer an alternative approach to developing new treatment strategies. The aim of our study is to evaluate significant correlations among deregulated miRNAs in advanced laryngeal carcinoma and to analyze, in silico, their strength of association, targets, and the most deregulated pathways. Several miRNAs demonstrated promising co-expression results, specifically miR-93-5p, miR-145-5p, and miR-210-3p. Their expressions were explored and further validated in a large set of in vivo advanced LSCC samples, which were subsequently used for bioinformatics and enrichment analyses. Our results highlight the significant roles of miR-93-5p, miR-145-5p, and miR-210-3p in regulating major pathways linked to the cell cycle via epithelial-to-mesenchymal transition (EMT), PI3K/Akt signaling, hypoxia, metabolism, apoptosis, angiogenesis, and metastasis. The associations between the expressions of these miRNAs and patients' clinical features could be central to the progression of advanced LSCC. Overall, our study provides important insights into the co-expression and regulatory networks of miR-93-5p, miR-145-5p, and miR-210-3p in advanced laryngeal carcinoma, underscoring their potential as therapeutic targets or biomarkers for this aggressive cancer. Further research is needed to elucidate the specific mechanisms through which these miRNAs contribute to the pathogenesis and progression of laryngeal carcinoma.
Regulatory role and molecular mechanism of METTL14 in vascular endothelial cell injury in preeclampsia
Authors: Wei Huafang , Liang Lin , Song Chengwen , Tong Ming , Xu Xiang
Published: September 2024

Preeclampsia (PE) is a pregnancy-related disease characterized by vascular endothelial cell injury. This study aimed to investigate the role of methyltransferase-like protein 14 (METTL14) in vascular endothelial cell injury in PE. A PE cell model was established by treating human umbilical vein endothelial cells (HUVECs) with tumor necrosis factor-alpha (TNF-α) in vitro. METTL14 and forkhead box protein 1 (FOXP1) were silenced, and miR-34a-5p was overexpressed in HUVECs to evaluate their effects. HUVEC viability, apoptosis, and levels of intercellular adhesion molecule 1, vascular cell adhesion molecule 1, and endothelin-1 were measured. The N6-methyladenosine (m6A) modification of pri-miR-34a-5p was quantified. The interactions between miR-34a-5p, DiGeorge syndrome critical region 8, and m6A enrichment in miR-34a-5p were analyzed. The relationship between miR-34a-5p and FOXP1 was also verified. The results showed the expressions of METTL14, FOXP1, and miR-34a-5p. METTL14 expression was elevated in the TNF-α-induced HUVEC injury model. Silencing METTL14 improved HUVEC viability, inhibited apoptosis, and reduced endothelial inflammation. METTL14 promoted miR-34a-5p expression through m6A modification. Overexpression of miR-34a-5p or silencing FOXP1 reversed the protective effects of METTL14 silencing on cell injury in the PE model. In conclusion, METTL14 mediated m6A modification to promote miR-34a-5p expression, leading to FOXP1 inhibition, which aggravated endothelial cell damage in the PE cell model.