bluuebunny/crossref_metadata_2025 huggingface dataset:
Bayesian Estimation of the Prevalence and Test Characteristics (Sensitivity and Specificity) of Two Serological Tests (RB and SAT-EDTA) for the Diagnosis of Bovine Brucellosis in Small and Medium Cattle Holders in Ecuador
Authors: Paucar Valeria , Ron-Román Jorge , Benítez-Ortiz Washington , Celi Maritza , Berkvens Dirk , Saegerman Claude , Ron-Garrido Lenin
Published: August 2021

In Ecuador, a national program for bovine brucellosis control has been in implementation since 2008. Given the costs, small- and medium-sized livestock holders are not completely committed to it. The objective of this study was to determine true prevalence (TP) of bovine brucellosis in small- and medium-sized herd populations, as well as the diagnostic sensitivity and specificity of the Rose Bengal (RB) test and the sero-agglutination test (SAT)-EDTA using a Bayesian approach. Between 2011 and 2016, 2733 cattle herds were visited, and 22,592 animal blood samples were taken in nineteen provinces on mainland Ecuador. Bayes-p and deviance information criterion (DIC) statistics were used to select models. Additionally, risk-factor analysis was used for herds according to their brucellosis test status. True prevalence (TP) in herds was estimated by pool testing. National seroprevalence of farms was 7.9% (95% CI: 6.79–9.03), and TP was 12.2% (95% CI: 7.8–17.9). Apparent prevalence (AP) in animals was 2.2% (95% CI: 1.82–2.67), and TP was 1.6% (95% CrI: 1.0–2.4). Similarly, the sensitivity of the RB was estimated at 64.6% (95% CrI: 42.6–85.3) and specificity at 98.9% (95% CrI: 98.6–99.0); for the SAT-EDTA test, sensitivity was 62.3% (95% CrI: 40.0–84.8) and 98.9% (95% CrI: 98.6–99.1) for specificity. Results of the two tests were highly correlated in infected and uninfected animals. Likewise, high spatial variation was observed, with the Coastal Region being the zone with the highest TP at 2.5%. (95% CrI: 1.3–3.8%) in individual animals and 28.2% (95% CI: 15.7–39.8) in herds. Risk factors include herd size, type of production (milk, beef, and mixed), abortions recorded, and vaccination. The results of this study serve to guide authorities to make decisions based on parallel testing at the beginning of a bovine brucellosis program for small livestock holders to increase sensitivity level of the screening tests in Ecuador.
Sulfidogenic Microbial Communities of the Uzen High-Temperature Oil Field in Kazakhstan
Authors: Sokolova Diyana S. , Semenova Ekaterina M. , Grouzdev Denis S. , Bidzhieva Salimat K. , Babich Tamara L. , Loiko Nataliya G. , Ershov Alexey P. , Kadnikov Vitaly V. , Beletsky Alexey V. , Mardanov Andrey V. , Zhaparov Nurlan S. , Nazina Tamara N.
Published: August 2021

Application of seawater for secondary oil recovery stimulates the development of sulfidogenic bacteria in the oil field leading to microbially influenced corrosion of steel equipment, oil souring, and environmental issues. The aim of this work was to investigate potential sulfide producers in the high-temperature Uzen oil field (Republic of Kazakhstan) exploited with seawater flooding and the possibility of suppressing growth of sulfidogens in both planktonic and biofilm forms. Approaches used in the study included 16S rRNA and dsrAB gene sequencing, scanning electron microscopy, and culture-based techniques. Thermophilic hydrogenotrophic methanogens of the genus Methanothermococcus (phylum Euryarchaeota) predominated in water from the zone not affected by seawater flooding. Methanogens were accompanied by fermentative bacteria of the genera Thermovirga, Defliviitoga, Geotoga, and Thermosipho (phylum Thermotogae), which are potential thiosulfate- or/and sulfur-reducers. In the sulfate- and sulfide-rich formation water, the share of Desulfonauticus sulfate-reducing bacteria (SRB) increased. Thermodesulforhabdus, Thermodesulfobacterium, Desulfotomaculum, Desulfovibrio, and Desulfoglaeba were also detected. Mesophilic denitrifying bacteria of the genera Marinobacter, Halomonas, and Pelobacter inhabited the near-bottom zone of injection wells. Nitrate did not suppress sulfidogenesis in mesophilic enrichments because denitrifiers reduced nitrate to dinitrogen; however, thermophilic denitrifiers produced nitrite, an inhibitor of SRB. Enrichments and a pure culture Desulfovibrio alaskensis Kaz19 formed biofilms highly resistant to biocides. Our results suggest that seawater injection and temperature of the environment determine the composition and functional activity of prokaryotes in the Uzen oil field.
Tailed Lytic Bacteriophages of Soft Rot Pectobacteriaceae
Authors: Miroshnikov Konstantin A. , Evseev Peter V. , Lukianova Anna A. , Ignatov Alexander N.
Published: August 2021

The study of the ecological and evolutionary traits of Soft Rot Pectobacteriaceae (SRP) comprising genera Pectobacterium and Dickeya often involves bacterial viruses (bacteriophages). Bacteriophages are considered to be a prospective tool for the ecologically safe and highly specific protection of plants and harvests from bacterial diseases. Information concerning bacteriophages has been growing rapidly in recent years, and this has included new genomics-based principles of taxonomic distribution. In this review, we summarise the data on phages infecting Pectobacterium and Dickeya that are available in publications and genomic databases. The analysis highlights not only major genomic properties that assign phages to taxonomic families and genera, but also the features that make them potentially suitable for phage control applications. Specifically, there is a discussion of the molecular mechanisms of receptor recognition by the phages and problems concerning the evolution of phage-resistant mutants.
Episodic Precipitation of Wolframite during An Orogen: The Echassières District, Variscan Belt of France
Authors: Monnier Loïs , Melleton Jérémie , Vanderhaeghe Olivier , Salvi Stefano , Lach Philippe , Bruguier Olivier , Benmammar Anissa , Bailly Laurent , Béziat Didier , Gloaguen Eric
Published: August 2021

Monazite and rutile occurring in hydrothermally altered W mineralizations, in the Echassières district of the French Massif Central (FMC), were dated by U-Pb isotopic systematics using in-situ Laser ablation-inductively coupled plasma–quadrupole mass spectrometry (LA-ICP-MS). The resulting dates record superimposed evidence for multiple percolation of mineralizing fluids in the same area. Cross-referencing these ages with cross-cutting relationships and published geochronological data reveals a long history of more than 50 Ma of W mineralization in the district. These data, integrated in the context of the Variscan belt evolution and compared to other major W provinces in the world, point to an original geodynamic-metallogenic scenario. The formation, probably during the Devonian, of a quartz-vein stockwork (1st generation of wolframite, called wolframite “a”; >360 Ma) of porphyry magmatic arc affinity is analogous to the Sn-W belts of the Andes and the Nanling range in China. This stockwork was affected by Barrovian metamorphism, induced by tectonic accretion and crustal thickening, during the middle Carboniferous (360 to 350 Ma). Intrusion of a concealed post-collisional peraluminous Visean granite, at 333 Ma, was closely followed by precipitation of a second generation of wolframite (termed “b”), from greisen fluids in the stockwork and host schist. This W-fertile magmatic episode has been widely recorded in the Variscan belt of central Europe, e.g. in the Erzgebirge, but with a time lag of 10–15 Ma. During orogenic collapse, a third magmatic episode was characterized by the intrusion of numerous rare-metal granites (RMG), which crystallized at ~310 Ma in the FMC and in Iberia. One of these, the Beauvoir granite in the Echassières district, led to the formation of the wolframite “c” generation during greisen alteration.
On Improving Wear Resistance of Cr-Al-N Coatings Using Dynamic Glancing Angle DC Magnetron Sputtering
Authors: Avila Pedro Renato Tavares , Apolinário Raíra Chefer , Rodrigues Alisson Mendes , Fernandes Jucielle Veras , Menezes Romualdo Rodrigues , Neves Gelmires de Araújo , Pinto Haroldo Cavalcanti
Published: August 2021

The development of alternatives for wear protection in surface engineering can be responsible for a significant decrease in energy waste as a large amount of the energy produced in the world is lost due to tribological contact. Dynamic Glancing Angle Deposition has been recently evaluated as a route to produce coatings with improved wear performance. In this technique, the substrate oscillates along with a determined range in front of the sputtering target during the growth of the film. In this study, five oscillatory ranges (0, ±5°, ±10°, ±15°, ±20°) were probed to manufacture nanostructured Cr-Al-N coatings using direct current magnetron sputtering, and their impact was investigated on the grain morphology, phase formation, chemical composition, and performance of the coatings. FEG-SEM revealed the formation of multilayer-like architecture across the grains of the coatings. The deposition rate and hardness improved, and a more than 2-fold decrease in the material loss was observed in a comparison between the stationary-deposited conventional coating and the sample produced under ±10° oscillatory range. This indicated the potential use of this technique in future surface engineering applications.
Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis
Authors: Behera Sujit Kumar , Sabarinath Thankappan , Mishra Prasanta Kumar K. , Deneke Yosef , Kumar Ashok , ChandraSekar Shanmugam , Senthilkumar Kuppusamy , Verma MedRam , Ganesh Balasubramanian , Gurav Amol , Hota Abhishek
Published: August 2021

Leptospirosis is responsible for hampering the productivity of swine husbandry worldwide. The aim of this study was to assess the efficacy of bioinformatics tools in predicting the three-dimensional structure and immunogenicity of recombinant LigBCon1-5 (rLigBCon1-5) antigen. A battery of bioinformatics tools such as I-TASSER, ProSA and SAVES v6.0 were used for the prediction and assessment of the predicted structure of rLigBCon1-5 antigen. Bepipred-2.0, DiscoTope v2.0 and ElliPro servers were used to predict linear and conformational epitopes while T-cell epitopes were predicted using NetMHCpan 4.1 and IEDB recommended 2.22 method for MHC Class I and II peptides respectively. The results obtained using various in silico methods were then compared with wet lab experiments comprising of both primary (IgG Dot ELISA Dipstick test) and secondary-binding assays (Latex Agglutination Test [LAT]) to screen 1153 porcine serum samples. The three-dimensional structure of rLigA/BCon1-5 protein as predicted by I-TASSER was found to be reliable by Ramachandran Plot and ProSA. The ElliPro server suggested 10 and three potential linear and conformational B-cell-epitopes, respectively, on the peptide backbone of the rLigA/BCon1-5 protein. The DiscoTope prediction server suggested 47 amino acid residues to be part of B-cell antigen. Ten of the most efficient peptides for MHC-I and II grooves were predicted by NetMHCpan 4.1 and IEDB recommended 2.22 method, respectively. Of these, three peptides can serve dual functions as it can fit both MHC I and II grooves, thereby eliciting both humoral-and cell-mediated immune responses. The prediction of these computational approaches proved to be reliable since rLigBCon1-5 antigen-based IgG Dot ELISA Dipstick test and LAT gave results in concordance to gold standard test, the Microscopic Agglutination Test (MAT), for serodiagnosis of leptospirosis. Both the IgG Dot ELISA Dipstick test and LAT were serodiagnostic assays ideally suited for peripheral level of animal health care system as “point of care” tests for the detection of porcine leptospirosis.
Piriformospora indica Primes Onion Response against Stemphylium Leaf Blight Disease
Authors: Roylawar Praveen , Khandagale Kiran , Randive Pragati , Shinde Bharat , Murumkar Chandrashekhar , Ade Avinash , Singh Major , Gawande Suresh , Morelli Massimiliano
Published: August 2021

The root-endophytic fungus Piriformospora indica (=Serendipita indica) has been revealed for its growth-promoting effects and its capacity to induce resistance in a broad spectrum of host plants. However, the bioefficacy of this fungus had not yet been tested against any pathogen affecting onion (Allium cepa). In this study, the biocontrol potency of P. indica against onion leaf blight, an impacting disease caused by the necrotrophic fungal pathogen Stemphylium vesicarium, was evaluated. First, it was proved that colonisation of onion roots by P. indica was beneficial for plant growth, as it increased leaf development and root biomass. Most relevantly, P. indica was also effective in reducing Stemphylium leaf blight (SLB) severity, as assessed under greenhouse conditions and confirmed in field trials in two consecutive years. These investigations could also provide some insight into the biochemical and molecular changes that treatment with P. indica induces in the main pathways associated with host defence response. It was possible to highlight the protective effect of P. indica colonisation against peroxidative damage, and its role in signalling oxidative stress, by assessing changes in malondialdehyde and H2O2 content. It was also showed that treatment with P. indica contributes to modulate the enzymatic activity of superoxide dismutase, catalase, phenylalanine ammonia-lyase and peroxidase, in the course of infection. qPCR-based expression analysis of defence-related genes AcLOX1, AcLOX2, AcPAL1, AcGST, AcCHI, AcWRKY1, and AcWRKY70 provided further indications on P. indica ability to induce onion systemic response. Based on the evidence gathered, this study aims to propose P. indica application as a sustainable tool for improving SLB control, which might not only enhance onion growth performance but also activate defence signalling mechanisms more effectively, involving different pathways.
Measurement of the IgG Avidity Index in the Diagnosis of Clinical Toxocariasis Patients
Authors: Menu Estelle , Kopec Lora , Luciani Léa , Legrand Sophie , L’Ollivier Coralie
Published: August 2021

Toxocara spp. are parasitic nematodes responsible for human toxocariasis, a common zoonotic helminth infection. The five main features of human toxocariasis are the classical ocular toxocariasis and visceral larva migrans syndrome, followed by covert toxocariasis, common toxocariasis and neurotoxocariasis. The diagnosis of toxocariasis is feasible by considering clinical symptoms, anamnestic history and serology laboratory results; however, serological criteria cannot be used to distinguish active Toxocara infection from past exposure, which is an area of much discussion in clinical practice. In this context, we developed avidity tests (ELISA and immunoblotting) and evaluated their clinical usefulness in distinguishing past from active toxocariasis. Our study involved 46 patients divided into two groups: “active toxocariasis” (n = 14) and “chronic toxocariasis” (n = 32). According to the avidity indices obtained for both the chronic and active toxocariasis groups, we proposed two thresholds: first, an AI lower than 32% supports an active infection; secondly, a threshold above 42% can exclude an active infection. In order to use this assay in routine clinical practice, however, is still requires standardisation with regards to the method and threshold values, which can be established through studies involving larger populations.
Evaluation of a PlexZyme-Based PCR Assay and Assessment of COVID-19 Surge Testing Throughput Compared to Cobas SARS-CoV-2
Authors: Pryce Todd M. , Haygarth Erin J. , Bordessa Jessica , Boan Peter A.
Published: August 2021

Reliable high-throughput methods are required for the detection of severe acute respiratory coronavirus 2 (SARS-CoV-2). We evaluated the new research use only (RUO) SpeeDx PlexZyme SARS-CoV-2 components (Plex) compared to the Roche cobas SARS-CoV-2 assay (cobas). A collection of positive (n = 214) and negative samples (n = 201) was tested in parallel comparing Plex with cobas. The overall agreement comparing the qualitative outcomes was 96.9%. Using an in-house quantitative PCR method, correlation comparing Plex ORF1ab to cobas ORF1a was r2 = 0.95. The median Plex ORF1ab change in target copy number compared to cobas ORF1a was +0.48 log10 copies/mL respectively. Inter- and intra-assay reproducibility of each assay was compared, including a limit-of-detection study. Reproducibility was comparable; however cobas was more sensitive than Plex by 1-log dilution. Throughput was evaluated during a COVID-19 testing surge of 4324 samples in a 30-h period. Plex demonstrated less hands-on time per reportable result (19% decrease) and increased throughput (155% increase of 102 results/hour) compared to cobas (40 results/hour). Our study demonstrates good qualitative and quantitative correlation of Plex compared to cobas and that Plex is well-suited for high throughput testing.
Special Issue on “Advancements in Fiber Bragg Grating Research”
Authors: Zhang Wei , Shu Xuewen
Published: August 2021

Since the discovery of photosensitivity in optical fibers, there has been great interest in fiber Bragg grating (FBG) research [...]
Towards Promising Platform by Using Annular Photonic Crystals to Simulate and Design Useful Mask
Authors: Ameen Ayman , Elsayed Hussein , Alamri Sagr , Matar Z.S. , Al-Dossari M. , Aly Arafa
Published: August 2021

Human masks are considered the mainstay in air filtration and purification technologies and against the spreading of bacterial and viral infections. This paper introduces a novel design of a human mask to increase the ultraviolet germicidal irradiation effect on pathogens. The proposed design consists of a tube with an annular photonic crystal (APC) attached to the mask’s orifice, and a UV source is located in the tube’s center. The main role of this study is the enhancement of UV doses based on the reflectivity of the proposed APC. Therefore, increasing pathogens’ inactivation level in the incoming air to the mask’s orifice could be investigated. The numerical investigations demonstrated that the proposed APC could provide a complete photonic bandgap with a high reflectivity in the wavelength regime from 207 to 230 nm. In addition, we have considered the roles of the thickness of layers, inner core radius, and the azimuthal number. Meanwhile, the results showed the ability to use a wide range of core radius values without almost any variations in the optical properties of the proposed design. Such results could grant the advantage of using this design by the manufacturing of human masks with different sizes besides the inclusions in other ultraviolet germicidal irradiation applications.
Dosage-Dependent Gynoecium Development and Gene Expression in Brassica napus-Orychophragmus violaceus Addition Lines
Authors: Cai Bowei , Wang Tai , Fu Wenqin , Harun Arrashid , Ge Xianhong , Li Zaiyun
Published: August 2021

Distant hybridization usually leads to female sterility of the hybrid but the mechanism behind this is poorly understood. Complete pistil abortion but normal male fertility was shown by one Brassica napus-Orychophragmus violaceus monosomic alien addition line (MA, AACC + 1 IO, 2n = 39) produced previously. To study the effect of a single O. violaceus chromosome addition on pistil development in different genetic backgrounds, hybrids between the MA and B. carinata (BBCC), B. juncea (AABB), and two synthetic hexaploids (AABBCC) were firstly produced in this study which show complete female sterility. A microspore culture was further performed to produce the haploid monosomic alien addition line (HMA, AC + 1 IO, 2n = 20) and disomic addition line (DA, AACC + 2 IO, 2n = 40) together with haploid (H, AC, 2n = 19) and double haploid (DH, AACC, 2n = 38) plants of B. napus from MA to investigate the dosage effect of the alien O. violaceus chromosome on pistil development and gene expression. Compared to MA, the development of the pistils of DA and HMA was completely or partially recovered, in which the pistils could swell and elongate to a normal shape after open pollination, although no seeds were produced. Comparative RNA-seq analyses revealed that the numbers of the differentially expressed genes (DEGs) were significantly different, dosage-dependent, and consistent with the phenotypic difference in pairwise comparisons of HMA vs. H, DA vs. DH, MA vs. DH, MA vs. DA, and MA vs. HMA. The gene ontology (GO) enrichment analysis of DEGs showed that a number of genes involved in the development of the gynoecium, embryo sac, ovule, and integuments. Particularly, several common DEGs for pistil development shared in HMA vs. H and DA vs. DH showed functions in genotoxic stress response, auxin transport, and signaling and adaxial/abaxial axis specification. The results provided updated information for the molecular mechanisms behind the gynoecium development of B. napus responding to the dosage of alien O. violaceus chromosomes.
Stem and Leaf Anatomy of Aragoa (Plantaginaceae): In Search of Lost Rays
Authors: Oskolski Alexei , Vuza Nathi , Shipunov Alexey
Published: August 2021

Aragoa is a shrubby genus endemic to páramo in the northern Andes representing the sister group to Plantago and Limosella. Stem and leaf structure of Aragoa corrugatifolia were studied to clarify the evolutionary pathways and ecological significance of their anatomical traits. Aragoa and Plantago share a non-fascicular primary vascular system, rayless wood and secondary phloem, and anomocytic stomata. Aragoa is distinctive from most Plantaginaceae in the presence of cortical aerenchyma and of helical thickenings in vessels. Its procambium emerges in the primary meristem ring as a continuous cylinder. The view on the ring meristem and procambial strands as developmental stages in the formation of a primary vascular system is not relevant for Aragoa, and probably for other Plantaginaceae. The raylessness is synapomorphic for the crown clade of Plantaginaceae comprising Aragoa, Littorella, Plantago, Veronica, Picrorhiza, Wulfenia, and Veronicastrum. The loss of rays is thought to be predetermined by procambium rather than by the vascular cambium. The extremely narrow vessels with helical thickenings are presumably adaptive to hydric and thermic conditions of páramo. Cortical aerenchyma is thought to be a response to the local hypoxia caused by the water retained by ericoid leaves. Trichomes on juvenile leaves are expected to be the traits of considerable taxonomic importance.
A 3D Food Printing Process for the New Normal Era: A Review
Authors: Lee Jinyoung
Published: August 2021

Owing to COVID-19, the world has advanced faster in the era of the Fourth Industrial Revolution, along with the 3D printing technology that has achieved innovation in personalized manufacturing. Three-dimensional printing technology has been utilized across various fields such as environmental fields, medical systems, and military materials. Recently, the 3D food printer global market has shown a high annual growth rate and is a huge industry of approximately one billion dollars. Three-dimensional food printing technology can be applied to various food ranges based on the advantages of designing existing food to suit one’s taste and purpose. Currently, many countries worldwide produce various 3D food printers, developing special foods such as combat food, space food, restaurants, floating food, and elderly food. Many people are unaware of the utilization of the 3D food printing technology industry as it is in its early stages. There are various cases using 3D food printing technology in various parts of the world. Three-dimensional food printing technology is expected to become a new trend in the new normal era after COVID-19. Compared to other 3D printing industries, food 3D printing technology has a relatively small overall 3D printing utilization and industry size because of problems such as insufficient institutionalization and limitation of standardized food materials for 3D food printing. In this review, the current industrial status of 3D food printing technology was investigated with suggestions for the improvement of the food 3D printing market in the new normal era.
Regulation of Eukaryote Metabolism: An Abstract Model Explaining the Warburg/Crabtree Effect
Authors: Gibart Laetitia , Khoodeeram Rajeev , Bernot Gilles , Comet Jean-Paul , Trosset Jean-Yves
Published: August 2021

Adaptation of metabolism is a response of many eukaryotic cells to nutrient heterogeneity in the cell microenvironment. One of these adaptations is the shift from respiratory to fermentative metabolism, also called the Warburg/Crabtree effect. It is a response to a very high nutrient increase in the cell microenvironment, even in the presence of oxygen. Understanding whether this metabolic transition can result from basic regulation signals between components of the central carbon metabolism are the the core question of this work. We use an extension of the René Thomas modeling framework for representing the regulations between the main catabolic and anabolic pathways of eukaryotic cells, and formal methods for confronting models with known biological properties in different microenvironments. The formal model of the regulation of eukaryote metabolism defined and validated here reveals the conditions under which this metabolic phenotype switch occurs. It clearly proves that currently known regulating signals within the main components of central carbon metabolism can be sufficient to bring out the Warburg/Crabtree effect. Moreover, this model offers a general perspective of the regulation of the central carbon metabolism that can be used to study other biological questions.
Risk Factors for Early and Late Onset Preeclampsia in Reunion Island: Multivariate Analysis of Singleton and Twin Pregnancies. A 20-Year Population-Based Cohort of 2120 Preeclampsia Cases
Authors: Robillard Pierre-Yves , Boukerrou Malik , Dekker Gustaaf , Scioscia Marco , Bonsante Francesco , Boumahni Brahim , Iacobelli Silvia
Published: August 2021

Objectives: To develop a multivariate model for risk factors specific to early onset preeclampsia (EOP) and late onset preeclampsia (LOP) in our entire population (singleton and twin pregnancies). Material and methods: 20 year-observational population-based historical cohort study (2001–2020). All consecutive births delivered at the Centre Hospitalier Universitaire Hospitalier Sud Reunion’s maternity ward. A standardized validated epidemiological perinatal database was used. Results: During the 20-year period, there were 81,834 pregnancies and 83,497 infants born, 1232 dichorionic and 350 monochorionic twin pregnancies. There were 2120 cases of preeclampsia, of which 2001 were preeclamptic singleton pregnancies and 119 twin pregnancies (incidence 7.5% in twin pregnancies vs. 2.5% singletons, OR 3.0, p < 0.001). Independent risk factors for EOP and LOP in a multivariate model (controlling for the two major confounders: maternal ages—both risks for EOP and LOP, and maternal pre-pregnancy BMI—specific risk factor for LOP) were: history of preeclampsia (adjusted OR (aOR) 11.7 for EOP, 7.8 for LOP, p < 0.0001), chronic hypertension (aOR 7.3 for EOP, 3.9 for LOP, p < 0.0001), history of perinatal death (aOR 2.2 for EOP, p < 0.0001 and 1.48 for LOP, p = 0.007), primipaternity (aOR 3.0 for EOP and 3.6 for LOP, p = 0.001), dizygotic twin pregnancies (aOR 3.7 for EOP, p < 0.0001 and 2.1 for LOP, p = 0.003), monozygotic twin pregnancies (aOR 3.98 for EOP, p = 0.003 and non-significant (NS) for LOP), ovulation induction (aOR 5.6 for EOP, p = 0.004 and NS for LOP), and in vitro fertilization (aOR 2.8 for EOP, p = 0.05 and NS for LOP). Specific to LOP and NS for EOP: renal diseases (aOR for LOP 2.9, p = 0.007) and gestational diabetes mellitus (aOR 1.2, p = 0.04). Conclusions: Maternal ages over 35 years, chronic hypertension, history of preeclampsia, ovulation induction, in vitro fertilizations, history of perinatal deaths and twin pregnancy (in our experience, especially mono zygotic twin pregnancies) are significant risk factors for EOP. New paternity is an independent factor for both EOP and LOP.
Assessing the Application-Specific Substitutability of Lithium-Ion Battery Cathode Chemistries Based on Material Criticality, Performance, and Price
Authors: Kiemel Steffen , Glöser-Chahoud Simon , Waltersmann Lara , Schutzbach Maximilian , Sauer Alexander , Miehe Robert
Published: August 2021

The material use of lithium-ion batteries (LIBs) is widely discussed in public and scientific discourse. Cathodes of state-of-the-art LIBs are partially comprised of high-priced raw materials mined under alarming ecological and social circumstances. Moreover, battery manufacturers are searching for cathode chemistries that represent a trade-off between low costs and an acceptable material criticality of the comprised elements while fulfilling the performance requirements for the respective application of the LIB. This article provides an assessment of the substitutability of common LIB cathode chemistries (NMC 111, −532, −622, −811, NCA 3%, −9%, LMO, LFP, and LCO) for five major fields of application (traction batteries, stationary energy storage systems, consumer electronics, power-/garden tools, and domestic appliances). Therefore, we provide a tailored methodology for evaluating the substitutability of products or components and critically reflect on the results. Outcomes show that LFP is the preferable cathode chemistry while LCO obtains the worst rating for all fields of application under the assumptions made (as well as the weighting of the considered categories derived from an expert survey). The ranking based on the substitutability score of the other cathode chemistries varies per field of application. NMC 532, −811, −111, and LMO are named recommendable types of cathodes.
Identification of Non-Traumatic Bone Marrow Oedema: The Pearls and Pitfalls of Dual-Energy CT (DECT)
Authors: Foti Giovanni , Serra Gerardo , Iacono Venanzio , Marocco Stefania , Bertoli Giulia , Gori Stefania , Zorzi Claudio
Published: August 2021

Dual-energy computed tomography (DECT) is an imaging technique widely used in traumatic settings to diagnose bone marrow oedema (BME). This paper describes the role of DECT in diagnosing BME in non-traumatic settings by evaluating its reliability in analyzing some of the most common painful syndromes. In particular, with an illustrative approach, the paper describes the possible use of DECT for the evaluation of osteochondral lesions of the knee and of the ankle, avascular necrosis of the hip, non-traumatic stress fractures, and other inflammatory and infectious disorders of the bones.
Double Trouble: Dengue Followed by COVID-19 Infection Acquired in Two Different Regions: A Doctor’s Case Report and Spatial Distribution of Cases in Presidente Prudente, São Paulo, Brazil
Authors: Pereira Sérgio Munhoz , do Nascimento Charlene Troiani , Ferro Rodrigo Sala , Flores Edilson Ferreira , Maldonado Bertacco Elaine Aparecida , Fonseca Elivelton da Silva , Prestes-Carneiro Luiz Euribel
Published: August 2021

Co-epidemics of COVID-19 and dengue in dengue-endemic countries represent a serious public health concern. In Brazil, São Paulo state ranks first for cases and deaths from COVID-19, and dengue is endemic in most regions of the country. In 2020, an outbreak of dengue occurred in western São Paulo. We determined the spatiotemporal distribution of dengue in the context of COVID-19 cases in Presidente Prudente, a mid-sized city in western São Paulo. To illustrate the burden of both infections, a case report of a doctor and his family, infected with dengue and COVID-19, is presented. There were three clusters of dengue and COVID-19 in the periphery. A dengue cluster was found in a region where there were no corresponding COVID-19 cases. Meanwhile, there were COVID-19 clusters where dengue activity was lower. In 2020, the COVID-19 epidemic emerged when dengue reached its seasonal peak, resulting in a simultaneous outbreak of both diseases. Lower rates of dengue were found in the city compared with 2019, and the fear of patients with mild dengue symptoms about remaining in hospital and acquiring COVID-19 infection may be the main cause. Simultaneous spatial clusters of dengue and COVID-19 in environmentally and socioeconomically vulnerable areas can guide public health authorities in intensive interventions to improve clinical diagnosis, epidemiological surveillance, and management of both diseases. The patient and his family were first infected with dengue and he then carried COVID-19 to his family, reinforcing the risk of health care workers spreading the virus to the community. We highlight the epidemiological significance of presenting a case report and spatial analysis of COVID-19 in the same study in the context of a dengue outbreak.
GERDA and LEGEND: Probing the Neutrino Nature and Mass at 100 meV and beyond
Authors: Cattadori Carla Maria , Salamida Francesco
Published: August 2021

The Gerda (GERmanium Detector Array) project, located at Laboratori Nazionali del Gran Sasso (LNGS), was started in 2005, a few years after the claim of evidence for the neutrinoless double beta decay (0νββ) of 76Ge to the ground state of 76Se: it is an ultra-rare process whose detection would directly establish the Majorana nature of the neutrino and provide a measurement of its mass and mass hierarchy. The aim of Gerda was to confirm or disprove the claim by an increased sensitivity experiment. After establishing the new technology of Ge detectors operated bare in liquid Argon and since 2011, Gerda efficiently collected data searching for 0νββ of 76Ge, first deploying the 76Ge-enriched detectors from two former experiments and later new detectors with enhanced signal-to-background rejection, produced from freshly 76Ge-enriched material. Since then, the Gerda setup has been upgraded twice, first in 2013–2015 and later in 2018. The period before 2013 is Phase I and that after 2015 is Phase II. Both the Gerda setup and the analysis tools evolved along the project lifetime, allowing to achieve the remarkable average energy resolution of ∼3.6 and ∼2.6 keV for Coaxial Germanium (Coax) detectors and for Broad Energy Germanium (BEGe), respectively, and the background index of 5.2−1.3+1.6 · 10−4 cts/(keV·kg·yr) in a 230 keV net range centered at Qββ. No evidence of the 0νββ decay at Qββ = 2039.1 keV has been found, hence the limit of 1.8·1026 yr on the half-life (T1/20ν) at 90% C.L. was set with the exposure of 127.2 kg·yr. The corresponding limit range for the effective Majorana neutrino mass mee has been set to 79–180 meV. The Gerda performances in terms of background index, energy resolution and exposure are the best achieved so far by 76Ge double beta decay experiments. In Phase II, Gerda succeeded in operating in a background free regime and set a world record. In 2017, the Legend Collaboration was born from the merging of the Gerda and Majorana Collaborations and resources with the aim to further improve the Gerda sensitivity. First, the Legend200 project, with a mass of up to 200 kg of 76Ge-enriched detectors, aims to further improve the background index down to <0.6 · 10−3 cts/(keV·kg·yr) to explore the Inverted Hierarchy region of the neutrino mass ordering, then the Legend1000 (1 ton of 76Ge-enriched) will probe the Normal Hierarchy. In this paper, we describe the Gerda experiment, its evolution, the data analysis flow, a selection of its results and technological achievements, and finally the design, features and challenges of Legend, the Gerda prosecutor.
Biological Effects of Air Pollution on Sensitive Bioindicators: A Case Study from Milan, Italy
Authors: Contardo Tania , Gazzotti Stefano , Ortenzi Marco Aldo , Vannini Andrea , Loppi Stefano
Published: August 2021

In this study, the physiological response of a sensitive lichen species (Evernia prunastri) exposed for three months in a complex urban area (Milan, Italy) was evaluated in order to verify if the air pollution abatement measures adopted over the years resulted in a suitable air quality for the survival of this sensitive species. Parameters investigated rely on the photosynthetic activity of the photobiont (Fv/Fm, PIabs, and OJIP curves), damage to mycobiont (membrane damage and antiradical activity), and the production of secondary metabolites involved in the protective functions of the organisms. Results showed that although air quality in Milan still suffers from heavy pollution from PM and NOx, the overall situation is not as severe as to induce the death of this sensitive biomonitor, at least in the short term. Nevertheless, the vital status of the samples exposed in the study area showed a significant impairment compared to that of samples exposed in a control area, indicating that the current air quality in Milan still prevents the optimal survival of E. prunastri.
Cutaneous Adverse Reactions to COVID-19 Vaccines: Insights from an Immuno-Dermatological Perspective
Authors: Niebel Dennis , Novak Natalija , Wilhelmi Jasmin , Ziob Jana , Wilsmann-Theis Dagmar , Bieber Thomas , Wenzel Joerg , Braegelmann Christine
Published: August 2021

(1) Background: Numerous vaccines are under preclinical and clinical development for prevention of severe course and lethal outcome of coronavirus disease 2019 (COVID-19). In light of high efficacy rates and satisfactory safety profiles, some agents have already reached approval and are now distributed worldwide, with varying availability. Real-world data on cutaneous adverse drug reactions (ADRs) remain limited. (2) Methods: We performed a literature research concerning cutaneous ADRs to different COVID-19 vaccines, and incorporated our own experiences. (3) Results: Injection site reactions are the most frequent side effects arising from all vaccine types. Moreover, delayed cutaneous ADRs may occur after several days, either as a primary manifestation or as a flare of a pre-existing inflammatory dermatosis. Cutaneous ADRs may be divided according to their cytokine profile, based on the preponderance of specific T-cell subsets (i.e., Th1, Th2, Th17/22, Tregs). Specific cutaneous ADRs mimic immunogenic reactions to the natural infection with SARS-CoV-2, which is associated with an abundance of type I interferons. (4) Conclusions: Further studies are required in order to determine the best suitable vaccine type for individual groups of patients, including patients suffering from chronic inflammatory dermatoses.
Survey on the Presence of Bacterial and Parasitic Zoonotic Agents in the Feces of Wild Birds
Authors: Ebani Valentina Virginia , Guardone Lisa , Bertelloni Fabrizio , Perrucci Stefania , Poli Alessandro , Mancianti Francesca
Published: August 2021

Wild avifauna may act as fecal source of bacterial and parasitic pathogens for other birds and mammals. Most of these pathogens have a relevant impact on human and livestock health which may cause severe disease and economic loss. In the present study, the fecal samples collected from 121 wild birds belonging to 15 species of the genera Anas, Tadorna, Fulica, Arddea, Larus, Falco, Athene, Accipiter, and Columba were submitted to bacteriological and molecular analyses to detect Brucella spp., Coxiella burnetii, Mycobacterium spp., Salmonella spp., Cryptosporidium spp., Giardia spp., and microsporidia. Four (3.3%) animals were positive for one pathogen: one Anas penelope for C. burnetii, one Larus michahellis for S. enterica serovar Coeln, and two Columba livia for Encephalitozoon hellem. Although the prevalence rates found in the present survey were quite low, the obtained results confirm that wild birds would be the a potential fecal source of bacterial and parasitic zoonotic pathogens which sometimes can also represent a severe threat for farm animals.
Lattice Boltzmann Method Study on Liquid Water Dynamic inside Gas Diffusion Layer with Porosity Distribution
Authors: Yang Mingyang , Du Aimin , Liu Jinling , Xu Sichuan
Published: August 2021

The gas diffusion layer (GDL) plays an important role in the mass transfer process during proton exchange membrane fuel cell (PEMFC) operation. However, the GDL porosity distribution, which has often been ignored in the previous works, influences the mass transfer significantly. In this paper, a 2D lattice Boltzmann method model is employed to simulate the liquid water transport process in the real GDL (considered porosity distribution) and the ideal GDL (ignore porous distribution), respectively. It was found that the liquid water transport in the real GDL will be significantly affected by the local low porosity area. In the real GDL, a liquid water saturation threshold can be noticed when the contact angle is about 118°. The GDL porosity distribution shows a stronger influence on liquid dynamic than hydrophobicity, which needs to be considered in future GDL modelling and design.
Risk of COVID-19 in shielded and nursing care home patients: a cohort study in general practice
Authors: Wingfield David , Taghavi Azar Sharabiani Mansour , Majeed Azeem , Molokhia Mariam
Published: August 2021

Background

COVID-19 cases were first detected in the UK in January 2020 and vulnerable patients were asked to shield from March to reduce their risk of COVID-19 infection.

Aim

To determine the risk and determinants of COVID-19 diagnosis in shielded versus non-shielded groups, adjusted for key comorbidities not explained by shielding.

Design & setting

Retrospective cohort study of adults with COVID-19 infection between 1 February 2020 and 15 May 2020 in west London.

Method

Individuals diagnosed with COVID-19 were identified in SystmOne records using clinical codes. Infection risks were adjusted for sociodemographic factors, nursing home status, and comorbidities.

Results

Of 57 713 adults, 573 (1%) individuals were identified as shielded and 1074 adults had documented COVID-19 infections (1.9%). COVID-19 infection rate in the shielded group individuals compared with non-shielded adult individuals was 6.5% (n = 37/573) versus 1.8% (n = 1037/57 140), P<0.001. A multivariable fully adjusted Cox proportional hazards (CPH) regression identified that COVID-19 infection was increased with shielding status (adjusted hazard ratio [aHR] 1.52; 95% confidence interval [CI] = 1.00 to 2.30; P = 0.048). Other determinants of COVID-19 infection included nursing home residency (aHR 7.05; 95% CI = 4.22 to 11.77; P<0.001); Black African (aHR 2.52; 95% CI = 1.99 to 3.18; P<0.001), Other (aHR 1.74; 95% CI = 1.42 to 2.13; P<0.001), Non-stated (aHR 1.70; 95% CI = 1.02 to 2.84; P = 0.04), or South Asian ethnic group (aHR 1.46; 95% CI = 1.10 to 1.93; P = 0.01); history of respiratory disease (aHR 1.51; 95% CI = 1.06 to 2.16; P = 0.02); deprivation (third versus least deprived Index of Multiple Deprivation [IMD] quintile) (aHR 1.25 ; 95% CI = 1.01 to 1.56; P = 0.05); obesity (body mass index [BMI] >30 kg/m2) (aHR 1.39; 95% CI = 1.18 to 1.63; P<0.001); and age (aHR 1.02; 95% CI = 1.01 to 1.02; P<0.001. Male sex was associated with lower risk of COVID-19 infection (aHR 0.71; 95% CI = 0.62 to 0.82; P<0.001).

Conclusion

Shielded individuals had a higher COVID-19 infection rate compared with non-shielded individuals, after adjusting for sociodemographic factors, nursing home status, and comorbidities.
Habitat of birds in Kambaramayana
Authors: R Ravikumar
Published: August 2021

Kambar one of the great epic story writer. In his one epic gives more importance about marine aquatic animals and their life styles. He also sang naturals beauty things like trees, animals, reptiles and give more or less, information about 50 types of birds. He tells about both animals and birds, but particular give clear information related to birds.  His information almost related to sangam literature works. So this present work give information about aquatic birds and their life habits based on kambar’s given information.
Assessment of Knowledge and Attitude Regarding Female and Male Breast Cancer among Adults of South India
Authors: Johns Anwitha , Kumar B P Satish , P R Lavanya
Published: August 2021

Background & Objectives: Breast cancer is the second leading reason for cancer death in women. Incidence rates of male breast cancer have increased by 0.2- 1% per year. The lack of knowledge and awareness of male breast cancer leads to its detection at a late stage in men. This study is to assess the knowledge and attitude of south Indian adults towards male and female breast cancer.
Methods: To assess the knowledge and attitude of adults on breast cancer, a questionnaire regarding basic knowledge and attitudes was formulated using Google forms. Numbers and percentages were formed to review categorical and nominal data. Chi-square (χ2) test was used for the comparison between the awareness of female breast cancer and male breast cancer. P < 0.05 was set as the level of significance.
Erratum: Immunoreactivity of estrogen receptor alpha in brain and ovary of the short mackerel Rastrelliger brachysoma (Bleeker, 1851).
Authors: Senarat Sinlapachai , Kettratad Jes , Kangwanrangsan Niwat , Jiraungkoorskul Wannee , Plumley Francis Gerald , Amano Masafumi , Shimizu Akio , Boonyoung Piyakorn , Kaneko Gen
Published: August 2021

The first author recognized an error within the manuscript and requested for a correction to be made for Figure 1(A) on page 54. The upper image of Figure 1(A) was a repetition of another manuscript that was published previously. The use of the same figure for another publication elsewhere is prohibited and therefore the new figure is printed below.
The Effect of Servant Leadership and Organizational Commitment on Organizational Citizenship Behavior: Job Satisfaction as Mediating Variable
Authors: Roza Wilia , Azka Bayu Pratama , Dharma Robby
Published: August 2021

The Effect of Servant Leadership and Organizational Commitment on Organizational Citizenship Behavior (OCB) with Job Satisfaction as an Intervening Variable at PT. Karya Semangat Mandiri Padang. This study aims to examine how much influence Servant Leadership and Organizational Commitment to Job Satisfaction and how much influence Servant Leadership, Organizational Commitment and Organizational Citizenship Behavior (OCB) on Job Satisfaction. Methods of data collection through surveys and distributing questionnaires, with a sample of 85 respondents obtained by the Slovin formula in the employee population of PT. Karya Semangat Mandiri Padang. The analysis method used is correlation analysis, multiple linear regression and path analysis using SPSS 16. The results obtained based on the T test (partial test) obtained Servant Leadership and Organizational Commitment parisally have a significant effect on the Organizational Citizenship Behavior (OCB) of PT. Karya Semangat Mandiri. Based on the F test (Simultaneous), it was found that Servant Leadership and Organizational Commitment parisally had a significant effect on job satisfaction of employees of PT. Karya Semangat Mandiri.