bluuebunny/crossref_metadata_2025 huggingface dataset:
Paracoccidioides brasiliensis Induces α3 Integrin Lysosomal Degradation in Lung Epithelial Cells
Authors: Almeida Bruna Rocha , Barros Bianca Carla Silva Campitelli , Barros Debora Tereza Lucas , Orikaza Cristina Mary , Suzuki Erika
Published: September 2023

Studies on the pathogen–host interaction are crucial for the understanding of the mechanisms involved in the establishment, maintenance, and spread of infection. In recent years, our research group has observed that the P. brasiliensis species interact with integrin family receptors and increase the expression of α3 integrin in lung epithelial cells within 5 h of infection. Interestingly, α3 integrin levels were reduced by approximately 99% after 24 h of infection with P. brasiliensis compared to non-infected cells. In this work, we show that, during infection with this fungus, α3 integrin is increased in the late endosomes of A549 lung epithelial cells. We also observed that the inhibitor of the lysosomal activity bafilomycin A1 was able to inhibit the decrease in α3 integrin levels. In addition, the silencing of the charged multivesicular body protein 3 (CHMP3) inhibited the reduction in α3 integrin levels induced by P. brasiliensis in A549 cells. Thus, together, these results indicate that this fungus induces the degradation of α3 integrin in A549 lung epithelial cells by hijacking the host cell endolysosomal pathway.
Ethical Implications of Chatbot Utilization in Nephrology
Authors: Garcia Valencia Oscar A. , Suppadungsuk Supawadee , Thongprayoon Charat , Miao Jing , Tangpanithandee Supawit , Craici Iasmina M. , Cheungpasitporn Wisit
Published: September 2023

This comprehensive critical review critically examines the ethical implications associated with integrating chatbots into nephrology, aiming to identify concerns, propose policies, and offer potential solutions. Acknowledging the transformative potential of chatbots in healthcare, responsible implementation guided by ethical considerations is of the utmost importance. The review underscores the significance of establishing robust guidelines for data collection, storage, and sharing to safeguard privacy and ensure data security. Future research should prioritize defining appropriate levels of data access, exploring anonymization techniques, and implementing encryption methods. Transparent data usage practices and obtaining informed consent are fundamental ethical considerations. Effective security measures, including encryption technologies and secure data transmission protocols, are indispensable for maintaining the confidentiality and integrity of patient data. To address potential biases and discrimination, the review suggests regular algorithm reviews, diversity strategies, and ongoing monitoring. Enhancing the clarity of chatbot capabilities, developing user-friendly interfaces, and establishing explicit consent procedures are essential for informed consent. Striking a balance between automation and human intervention is vital to preserve the doctor–patient relationship. Cultural sensitivity and multilingual support should be considered through chatbot training. To ensure ethical chatbot utilization in nephrology, it is imperative to prioritize the development of comprehensive ethical frameworks encompassing data handling, security, bias mitigation, informed consent, and collaboration. Continuous research and innovation in this field are crucial for maximizing the potential of chatbot technology and ultimately improving patient outcomes.
Molecular Profile of Variants in CDH1, TP53, PSCA, PRKAA1, and TTN Genes Related to Gastric Cancer Susceptibility in Amazonian Indigenous Populations
Authors: Aguiar Kaio Evandro Cardoso , Oliveira Izabela De Sousa , Cohen-Paes Amanda De Nazaré , Coelho Rita De Cássia Calderaro , Vinagre Lui Wallacy Morikawa Souza , Rodrigues Juliana Carla Gomes , Ribeiro-Dos-Santos André Maurício , De Souza Sandro José , Ribeiro-Dos-Santos Ândrea , Guerreiro João Farias , Assumpção Paulo Pimentel de , Santos Sidney Emanuel Batista Dos , Santos Ney Pereira Carneiro Dos , Fernandes Marianne Rodrigues
Published: September 2023

Gastric Cancer is a disease associated with environmental and genetic changes, becoming one of the most prevalent cancers around the world and with a high incidence in Brazil. However, despite being a highly studied neoplastic type, few efforts are aimed at populations with a unique background and genetic profile, such as the indigenous peoples of the Brazilian Amazon. Our study characterized the molecular profile of five genes associated with the risk of developing gastric cancer by sequencing the complete exome of 64 indigenous individuals belonging to 12 different indigenous populations in the Amazon. The analysis of the five genes found a total of 207 variants, of which 15 are new in our indigenous population, and among these are two with predicted high impact, present in the TTN and CDH1 genes. In addition, at least 20 variants showed a significant difference in the indigenous population in comparison with other world populations, and three are already associatively related to some type of cancer. Our study reaffirms the unique genetic profile of the indigenous population of the Brazilian Amazon and allows us to contribute to the conception of early diagnosis of complex diseases such as cancer, improving the quality of life of individuals potentially suffering from the disease.
Measurement and Calibration of Regulatory Credit Risk Asset Correlations
Authors: van Dyk Anton , van Vuuren Gary
Published: September 2023

Vasicek’s asymptotic single risk factor (ASRF) model is employed by the Basel Committee on Banking Supervision (BCBS) in its internal ratings-based (IRB) approach for estimating credit losses and regulatory credit risk capital. This methodology requires estimates of asset correlations; these are prescribed by the BCBS. Practitioners are interested to know market-implied asset correlations since these influence economic capital and lending behavior. These may be backed out from ASRF loan loss distributions using ex post loan losses. Prescribed asset correlations have been neither updated nor recalibrated since their introduction in 2008 with the implementation of the Basel II accord. The market milieu has undergone significant alterations and adaptations since then; it is unlikely that these remain relevant. Loan loss data from a developed (US) and developing (South Africa) economy spanning at least two business cycles for each region were used to explore the relevance of the BCBS calibration. Results obtained from three alternative methodologies are compared with prescribed BCBS values, and the latter were found to be countercyclical to empirical loan loss experience, resulting in less punitive credit risk capital requirements than required in market crises and more punitive requirements than required in calm conditions.
Nonlinear Flood Responses to Tide Level and Land Cover Changes in Small Watersheds
Authors: Huang Huabing , Pan Yu , Wang Changpeng , Wang Xianwei
Published: September 2023

Regarding global warming, the threat of flooding is projected to increase due to the change in intensity and frequency of single drivers and amplification caused by multi-driver interactions. This interaction becomes more complicated in developing regions with rapidly changing land cover. As a result, demands on flood risk management are rising especially in small watersheds, which are more vulnerable to driver disturbances compared with large watersheds. Existing studies focused on large watersheds rather than small watersheds. However, the findings derived from large-scale analysis cannot be transferred to small watersheds directly. This research investigated the flood responses in the Yonghe River Watershed (YRW) (63.8 km2) in Guangzhou, China, considering the impact of land cover change. The YRW experienced a disastrous compound flood on 22 May 2020. A hydrodynamic model integrating the Hydrologic Engineering Center’s Hydrologic Modeling System and River Analysis System (HEC-HMS and HEC-RAS, respectively) was established and calibrated using the inundation depths observed during the flood. Model analysis using multiple scenarios showed that the watershed is river-dominated, and flood responses to the three factors are nonlinear but with different increasing rates. The response curves for tide levels and land cover changes increase faster at high values, whereas the rainfall intensity curves vary slightly. These findings highlight the importance of integrating tidal impacts into flood risk management, even in river-dominated coastal watersheds. The study further recommends that in small watersheds, 50% imperviousness is an indicator of the urgent demand for flood risk management measures.
How Do Farmers Realize Their Rights on the Collective Land in Rural China? An Explanatory Framework for Deconstructing the Subject of Collective Land Ownership
Authors: Chen Yixiang , Jin Xiangmu
Published: September 2023

This study aims to deconstruct the collective, the subject of collective land ownership. With respect for the logic of the formation of collective land ownership, we propose the “transfreserve” mode to portray the division of rural land rights between the members and the organization in the transformation from private ownership to collective ownership. This idea can be expressed as, prompted by the public power of the state, each farmer as the owner of rural land having to transfer part of his/her rights to the organization when associating, meanwhile each one still reserves part of his/her rights. We term the rights transferred to the organization as special legal person ownership, while the rights reserved by each farmer are called membership rights. The rights exercised by all members on the basis of membership rights are the autonomous rights. In terms of the property rights, such as the distribution right of the collective income, farmers have to participate in decision-making to determine how to form the allocation scheme in a fair and reasonable way by exercising autonomous rights; then, organization fulfills the collective will to meet the needs of its members by exercising special legal person ownership. As for the right to use public infrastructure on the collective land, farmers, as the members, can use it reasonably by its own will, which is the process of exercising membership rights. If farmers’ rights are infringed by other members, they can choose to negotiate with other members in a proper way. If farmers’ rights are infringed when the organization carries out operation activity in the land market, they can obtain compensation from the organization, and the compensation standard is determined by the decision-making.
Unveiling the Role of Zoos in Smart Cities: A Quantitative Analysis of the Degree of Smartness in Kyoto City Zoo
Authors: Lin Yuxuan , Yang Ruochen , Shimoda Ryosuke , Xian Zheng , Liu Shuhao
Published: September 2023

The rapid pace of urbanization and the emergence of social challenges, including an aging population and increased labor costs resulting from the COVID-19 pandemic, have underscored the urgency to explore smart city solutions. Within these technologically advanced urban environments, zoos have assumed a pivotal role that extends beyond their recreational functions. They face labor cost challenges and ecological considerations while actively contributing to wildlife conservation, environmental education, and scientific research. Zoos foster a connection with nature, promote biodiversity awareness, and offer a valuable space for citizens, thereby directly supporting the pillars of sustainability, public engagement, and technological innovation in smart cities. This study employs a quantitative analysis to assess the alignment between smart projects and the distinctive characteristics of Kyoto Zoo. Through questionnaires, we collected feedback on performance and importance, and subsequently employed the analytic hierarchy process and the fuzzy integrated evaluation method to obtain quantitative results. The findings reveal the high level of intelligence exhibited by Kyoto Zoo, and the analysis provides insightful guidance that can be applied to other urban facilities. At the same time, we compared Kyoto Zoo with Ueno Zoo to see the difference in intellectualization achievements in different contexts in terms of data and systems.
Spatial Identification and Change Analysis of Production-Living-Ecological Space Using Multi-Source Geospatial Data: A Case Study in Jiaodong Peninsula, China
Authors: Ni Mingyan , Zhao Yindi , Ma Caihong , Jiang Wenzhi , Xie Yanmei , Hou Xiaolin
Published: September 2023

The significant heterogeneity in the spatial distribution of point of interest (POI) data, the absence of human socio-economic activity information in remote sensing images (RSI), and the high cost of land use (LU) data acquisition restrict their application in PLES spatial identification. Utilizing easily accessible data for detailed spatial identification of PLES remains an urgent challenge, especially when selecting a study area that encompasses both urban built-up areas (UBUA) and non-urban built-up areas (NUBUA). To address this issue, we proposed a PLES spatial identification method that combines POI data and land cover (LC) data in this paper. The proposed method first classified spatial analysis units (SAUs) into agricultural production space (APS), ecological space (ES), and ambiguous space (AS) based on the rich surface physical information from LC data. Subsequently, the AS was further classified into living space (LS) and non-agricultural production space (NAPS) based on the rich human socioeconomic information from POI data. For the AS that contains no POI, a simple rule was established to differentiate it into LS or NAPS. The effectiveness of the method was verified by accuracy evaluation and visual comparison. Applying the method to the Jiaodong Peninsula, we identified the PLES of the Jiaodong Peninsula for 2018 and 2022, further explored their spatial distribution characteristics, and analyzed their changes. Finally, we conducted a discussion on the real-world situations and driving mechanisms of the PLES changes and proposed several policy insights. The results indicated that both the spatial distribution characteristics of PLES and PLES change in the Jiaodong Peninsula were obvious and showed significant differentiation between UBUA and NUBUA. Climatic and natural resource conditions, geographic location, macro-policies, and governmental behaviors drove the PLES changes.
Potato Wart Isolates from Europe and North America Form Distinct Clusters of Genetic Variation
Authors: Röhrs Ina , Linde Marcus , Przetakiewicz Jaroslaw , Zelya Avrelia , Zelya George , Pucher Anna , Tlapák Hana , Debener Thomas
Published: September 2023

We have extended previously published sets of simple sequence repeat markers for Synchytrium endobioticum, selected to be polymorphic for the German-standard isolates of pathotypes P1, P2, P6, P8, and P18. These markers also complement the extensive published information on DNA polymorphisms for the mitogenomes of Synchytrium endobioticum. This extended set of 35 markers representing 73 alleles differentiated 51 isolates from Europe and North America into three large, well-separated clusters and subclusters using dendrogram analysis, principal coordinates analysis (PCoA), and population substructure analysis using STRUCTURE 2.3.4 software. This suggests a limited number of introgressions of the wart disease pathogen into current potato growing areas, followed by recombination and admixture of populations through human activities. The new markers extend the published marker sets and are useful tools for future analyses of population structure and dynamics in Synchytrium endobioticum, which are necessary to understand the biology of the interaction between the pathogen and its potato host and to develop future control strategies.
Eosinophil Cationic Protein Variation in Patients with Asthma and CRSwNP Treated with Dupilumab
Authors: Ledda Andrea Giovanni , Costanzo Giulia , Sambugaro Giada , Caruso Cristiano , Bullita Martina , Di Martino Maria Luisa , Serra Paolo , Firinu Davide , Del Giacco Stefano
Published: September 2023

Background: Asthma is a clinical syndrome characterized by recurrent episodes of airway obstruction, bronchial hyperresponsiveness and airway inflammation. Most patients with asthma present a “type 2” (TH2) inflammation. ILC2 and TH2 cells release cytokines IL4, IL-13 and IL-5. CRSwNP is a condition characterized by hyposmia or anosmia, nasal congestion, nasal discharge, and face pain or pressure that last for at least 12 weeks in a row without relief. Both asthma and CRSwNP are often characterized by a type 2 inflammation endotype and are often present in the same patient. Dupilumab is a fully human monoclonal antibody targeting the interleukin-4 receptor α (IL-4Rα) subunit, blocking IL4/IL-4Rα binding and IL13. It has been labelled for the treatment of moderate to severe asthma in patients from the age of 12 years with an eosinophilic phenotype, and it has demonstrated efficacy and acceptable safety. Our study aims to investigate the effects of dupilumab on type 2 inflammatory biomarkers, such as eosinophils and eosinophil cationic protein (ECP). ECP is an eosinophil-derived substance contained in granules that are released during inflammation and causes various biological effects, including tissue damage in asthmatic airways. Methods: ECP, Eosinophil counts (EOS), and total immunoglobulin E (IgE) levels were longitudinally measured using immunoassays in the serum of 21 patients affected by CRSwNP, of which 17 had asthma as a comorbidity, receiving 300 mg dupilumab every two weeks. Results: The EOS and ECP, after a first phase of significant increase due to the intrinsic characteristic of the block of IL-4 and IL-13, returned to the baseline 10 months after the initial administration of dupilumab. Fractional exhaled nitric oxide (FeNO) and serum total IgE decreased significantly after 9 months. Asthma Control Test (ACT) scores improved after dupilumab treatment. FEV1% and FEV1 absolute registered a significant improvement at 10 months. Conclusions: Patients who received 300 milligrams of dupilumab every two weeks first experienced a temporary increase in eosinophils (EOS) and eosinophil cationic protein (ECP), then exhibited a gradual decline in these variables with a subsequent return to the initial baseline levels. When compared to the baseline, we observed that the levels of IgE and FeNO decreased over time, while there was an increase in both FEV1 and FEV1%.
Optimization Workflow of Fumonisin Esterase Production for Biocatalytic Degradation of Fumonisin B1
Authors: Incze Dániel János , Poppe László , Bata Zsófia
Published: September 2023

Industrial enzyme production with the Pichia pastoris expression system requires a well-characterized production strain and a competitively priced fermentation medium to meet the expectations of the industry. The present work shows a workflow that allows the rapid and reliable screening of transformants of single copy insertion of the target production cassette. A constitutive expression system with the glyceraldehyde-3-phosphate dehydrogenase promoter (pGAP) with homology arms for the glycerol kinase 1 (GUT1) was constructed for the targeted integration of the expression plasmid in a KU70 deficient Pichia pastoris and the production of a bacterial fumonisin esterase enzyme (CFE). A robust colony qPCR method was developed for the copy number estimation of the expression cassette. Optimization of the protein production medium and the scale-up ability was aided by design of experiments (DOE) approach resulting in optimized production conditions at a semi-industrial scale. A novel fermentation medium containing 3% inactivated yeast and 2% dextrose in an ammonium-citrate buffer (IYD) was shown to be a promising alternative to YPD media (containing yeast extract, peptone, and dextrose), as similar protein titers could be obtained, while the cost of the medium was reduced 20-fold. In a demonstration-scale 48 h long fed-batch fermentation, the IYD media outperformed the small-scale YPD cultivation by 471.5 ± 22.6%.
An Investigation of the Sequential Micro-Laser Drilling and Conventional Re-Drilling of Angled Holes in an Inconel 625 Ni-Based Alloy
Authors: Szwajka Krzysztof , Zielińska-Szwajka Joanna , Żaba Krzysztof , Trzepieciński Tomasz
Published: September 2023

The conventional (mechanical) micro-drilling of Inconel 625 alloys suffers from premature breakage of the drill bit due to its brittle nature and limited cutting tool life. Even greater problems are encountered when micro-drilling holes at an acute angle to the machining plane. In such a process, there are great difficulties associated with the low stiffness of the tool, which leads to the frequent breakage of the drill during machining. Therefore, in this type of mechanical drilling operation, the hole surface is usually milled with an end mill to provide a flat surface on the entry side of the drill bit. The aim of this article is to recognise the process of sequential micro-drilling and to assess the possibility of its use as an effective and efficient method of micro-drilling in hard-to-cut metals. The paper describes the process of initial laser drilling followed by final mechanical micro-drilling. Inconel 625 Ni-based alloy sheets were used as the test material. The shape and microstructure of pre-holes made with a laser, the volumetric efficiency of laser processing, the energy in the mechanical drilling process, and tool wear were analysed. The research results show that in the sequential drilling process, mechanical re-drilling eliminates the geometrical discrepancies resulting from the laser pre-drilling. In addition, it was found that, compared to mechanical micro-drilling, the use of sequential micro-drilling resulted in a two-fold increase in drill life. It has been also observed that sequential machining reduces the energy demand by 60% compared to mechanical micro-drilling. In addition, it was found that the edge of the drill bit is a key factor in deciding the target diameter of the laser-drilled pilot hole, and thus in selecting the micro-drilling parameters.
4a′-Hydroxy-3′,3′,5,6′,6′,7-hexamethyl-3′,4′,4a′,6′,7′,9a′-hexahydrospiro[indole-3,9′-xanthene]-1′,2,8′(1H,2′H,5′H)-trione
Authors: Ryzhkova Yuliya E. , Kalashnikova Varvara M. , Ryzhkov Fedor V. , Fakhrutdinov Artem N. , Elinson Michail N.
Published: September 2023

Pseudo-multicomponent reactions (Pseudo-MCRs) have led to a variety of compounds with interesting biological properties, especially desirable in the pharmaceutical industry. The isatin nucleus could be considered a privileged scaffold for the design of biologically active substances. Dimedone is an interesting and versatile molecule for most organic transformations, especially one-pot and multicomponent reactions. Xanthene derivatives are still an attractive research field for both academia investigations and industry. In this investigation, a simple and efficient tandem Knoevenagel–Michael protocol with subsequent cyclization for the synthesis of the previously unknown 4a′-hydroxy-3′,3′,5,6′,6′,7-hexamethyl-3′,4′,4a′,6′,7′,9a′-hexahydrospiro[indole-3,9′-xanthene]-1′,2,8′(1H,2′H,5′H)-trione was elaborated. The suggested method is based on the pseudo-MCR of 5,7-dimethylisatin and dimedone. The structure of the earlier unknown compound was proven using 1H, 13C-NMR, and IR spectroscopy, mass spectrometry, and elemental analysis. To compare the developed protocol with the existing ones, unsubstituted spiro[indole-3,9′-xanthene] was synthesized. Its structure has been proven using two-dimensional (2D) NMR spectroscopy techniques.
Porous Structure and Fractal Dimensions of Activated Carbon Prepared from Waste Coffee Grounds
Authors: Sklepova Sofiia Victoriia , Ivanichok Nataliia , Kolkovskyi Pavlo , Kotsyubynsky Volodymyr , Boychuk Volodymyra , Rachiy Bogdan , Uhryński Andrzej , Bembenek Michał , Ropyak Liubomyr
Published: September 2023

The present work reports the results of a systematic study on the evolution of the morphological properties of porous carbons derived from coffee waste using a one-pot potassium-hydroxide-assisted process at temperatures in the range of 400–900 °C. Raw materials and obtained carbons were studied by TG, DTG, SEM and nitrogen adsorption porosimetry. The decomposition temperature ranges for hemicellulose, cellulose and lignin as the main component of the feedstock have been established. It is shown that the proposed method for the thermochemical treatment of coffee waste makes it possible to obtain activated carbon with a controllable pore size distribution and a high specific surface area (up to 1050 m2/g). A comparative study of the evolution of the distribution of pore size, pore area and pore volume has been carried out based on the BJH and NL-DFT (slit-like pores approximation) methods. The fractal dimension of the obtained carbons has been calculated by Frenkel–Halsey–Hill method for single-layer and multilayer adsorptions.
Design and Implementation of a Robotic Arm for a MoCap System within Extended Educational Mechatronics Framework
Authors: Lopez-Neri Emmanuel , Luque-Vega Luis F. , González-Jiménez Luis E. , Guerrero-Osuna Héctor A.
Published: September 2023

Educational mechatronics aims to be the evolution of educational robotics so it can be identified as a part of the educational paradigm of the university, its academic spaces, infrastructure, and practical activities. The fundamental goal of this framework is to develop the knowledge and skills that the new industrial world, inspired by the latest technologies, necessitates. This work proposes the modular design of a robotic arm aligned with the extended educational mechatronics conceptual framework by designing and implementing educational tools to develop the knowledge and skills required for Industry 4.0. The 3D-printed, low-cost robotic arm is designed to be used in a motion capture system for robotics applications to build kinematics concepts for a learning process. In particular, the instructional design to build the mechatronic concept of a robot workspace is carried out considering the three learning levels: concrete, representational, and abstract. The above demonstrates how the proposed pedagogical methodology can impact Industry 4.0 in the small- and medium-sized enterprises’ context.
Molecular Dynamics Calculations for the Temperature Response of Poly(alkylated tri(ethylene oxide)isocyanate) Aqueous Solution
Authors: Mizutani Shunsuke , Kita Shunya , Sakai Naoya , Yamamoto Takuya , Koleżyński Andrej , Kakuchi Toyoji , Sato Shin-ichiro
Published: September 2023

Aqueous solutions of conventional temperature-responsive amphiphilic polymers undergo a coil–globule conformational transition around the lower critical solution temperature (LCST) that causes the polymer surfaces to become hydrophobic and the polymers to aggregate together. Isocyanate polymers with alkylated oligo(ethylene oxide) side chains are expected to have rigid main chains and, thus, do not undergo the coil–globule structural transition, but they have recently been reported to exhibit temperature-responsive properties. In this study, molecular dynamics was used to calculate the agglomeration tendencies of two chains of poly(alkylated tri(ethylene oxide)isocyanate) (PRTEOIC, where R = methyl (Me) or ethyl (Et)) in aqueous solution to elucidate the LCST phenomenon in the absence of coil–globule conformational transition. Our MD simulations showed that aggregation also occurs in rod polymers. Furthermore, we found that both (PMeTEOIC)2 and (PEtTEOIC)2 showed parallel agglomeration of the two molecular chains with increasing temperature, but only (PMeTEOIC)2 showed a metastable T-shaped agglomeration in the middle temperature range. The crossing-point temperature (TCRP) at which the density of the first hydrophobic hydration shell around the sidechain alkyl group equals the bulk water density is a useful indicator for predicting the LCST of rod polymers with dense side chains terminated by alkyl groups.
Discriminating among Generalized Exponential, Weighted Exponential and Weibull Distributions
Authors: Niu Ruizheng , Tian Weizhong , Zhang Yunchu
Published: September 2023

In this paper, we consider the problem of discriminating among three different positively skewed lifetime distributions, namely the generalized exponential distribution, the weighted exponential distribution, and the Weibull distribution. All of these distributions have been used quite effectively to analyze positively skewed lifetime data. We use the methods of the ratio of maximized likelihood, the minimum Kolmogorov distance, and the sequential probability ratio test to discriminate among these three distributions. The probability of correct selection is considered for each hypothesis based on several scenarios with Monte Carlo simulation. Real data applications are studied to illustrate the effectiveness of these proposed methods.
Comparative Study of Hematological Parameters and Biomarkers of Immunity and Inflammation in Patients with Psoriasis and Atopic Dermatitis
Authors: Bakic Mirjana , Klisic Aleksandra , Karanikolic Vesna
Published: September 2023

Background and Objectives: There are no studies regarding comparative analysis of hematological parameters in patients with psoriasis (PsO) and atopic dermatitis (AD), whereas studies examining serum biomarkers of immunity and inflammation in these entities are scarce and contradictory. We aimed to compare such parameters in patients with PsO and AD. Materials and Methods: Patients with PsO (n = 40) and AD (n = 40) were consecutively included in this cross-sectional study. Hematological parameters and biomarkers of immunity and inflammation (interferon-gamma (IFN-γ), interleukine (IL)-22 and C-reactive protein (CRP)) were determined. Results: While the mean corpuscular volume (MCV) was higher in the PsO group vs. the AD group (p < 0.05), there was no difference in the other examined parameters between groups. A higher neutrophil-to-lymphocyte ratio (NLR) was found in patients with AD > 50 years vs. patients with PsO of similar age (p < 0.05). Higher IL-22 levels were found in patients with AD < 50 years vs. patients with PsO of similar age (p < 0.05). Lower IL-22 levels were found in patients with AD > 50 years vs. patients with AD < 50 years (p < 0.05). Patients with PsO and with comorbidities had lower platelets (PLT), plateletcrit (PCT) and platelet-to-lymphocyte ratio (PLR), whereas lymphocytes, red cell distribution width-to-PLT ratio (RPR) and mean platelet volume/PLT ratio (MPR) were higher vs. PsO patients without comorbidities. Patients with AD and with comorbidities had lower PCT and PLR, whereas RPR was higher vs. AD patients without comorbidities. Conclusions: A higher pro-inflammatory state (i.e., higher NLR and IL-22) was found in AD vs. PsO in age-specific groups. A higher pro-inflammatory state (i.e., as reflected by platelet indexes) was found in both diseases with comorbidities.
The Bone Bridge Technique Utilizing Bone from the Lateral Wall of the Maxillary Sinus for Ridge Augmentation: Case Reports of a 1–7 Year Follow-Up
Authors: Park Won-Bae , Han Ji-Young , Kang Philip
Published: September 2023

The post-extraction socket of a periodontally compromised tooth/implant is oftentimes accompanied by a very wide-deep alveolar ridge defect. The commonly utilized treatment is ridge preservation followed by delayed implant placement 4 to 6 months after extraction. In the four cases presented in this study, a novel technique of utilizing a bone block obtained from the lateral wall of the maxillary sinus is introduced. Due to the severe localized vertical ridge deficiency, an intraoral autogenous bone block was obtained from the ipsilateral sinus bony window. After the obtained bone block was properly trimmed, it was fixed in the form of a bridge over the vertical defect by the press-fit method. In two cases, the gap between the autogenous bone and defect was filled with a particulate synthetic bone graft, and in another two cases, the gap was left without grafting. All cases were covered with a resorbable collagen membrane. At the time of re-entry after 5 to 6 months, the bone bridge was well incorporated beside the adjacent native bone and helped by the implant placement. Uncovering was performed after 3 to 6 months, and prostheses were delivered after 2 months. Oral function was maintained without any change in the marginal bone level even after the 1- to 7-year post-prosthesis delivery. This case series showed that the bone bridge technique performed using an ipsilateral sinus bony window for a localized vertical deficiency of a post-extraction socket can be used for successful vertical ridge augmentation (VRA).
Population-Based Screening for Coeliac Disease in Lithuanian Children from 2009 to 2014
Authors: Urbonas Vaidotas , Sadauskaite Jolita , Varnas Dominykas
Published: September 2023

Background and Objectives. Coeliac disease is an autoimmune disorder provoked by a dietary group of proteins called gluten in genetically predisposed individuals. Over the past several decades, the prevalence of coeliac disease has been steadily growing and it is now recognized to be occurring worldwide. The prevalence varies greatly between ethnic, racial groups and regionally. Such variability makes local epidemiological studies important for spreading awareness and setting a threshold for suspicion of coeliac disease. We explored the potential application of a quick point-of-care test for the purpose of detecting a presence of IgA class TG2 antibodies for coeliac disease and screening in a Lithuanian pediatric population. Previously, there were no data regarding coeliac disease prevalence in Lithuania. Materials and Methods. Overall, we included 1458 children 11–13 years of age from several Lithuanian schools selected randomly in this study. Utilizing one point-of-care test using a single blood sample taken from a fingertip, we identified the existence of IgA class TG2 antibodies. Only children whose parents gave consent were enrolled in the study. Those with positive IgA class TG2-ab were directed to a tertiary hospital for additional clinical assessment and confirmation of suspected coeliac disease. Results. A total of two (0.14%) of the 1458 enrolled children were detected with the presence of TG2 antibodies and the coeliac disease diagnosis was further confirmed with histological examination of duodenal biopsy samples. Additionally, we checked that patients had not previously reported any clinical symptoms and signs that could suggest coeliac disease or any other disease of the gastrointestinal tract. Conclusions. The detected prevalence of coeliac disease in the Lithuanian pediatric population is 1:729. The rapid finger prick test for the presence of IgA class TG2 antibodies is a reasonable and accurate method to screen for celiac disease in children.
Outcomes of Gestational Trophoblastic Disease Management: A Single Centre Review
Authors: Malovrh Eva Pavla , Lukinovič Nuša , Sobočan Monika , Knez Jure
Published: September 2023

Background and Objectives: Gestational trophoblastic disease (GTD) is a group of pregnancy-related malignant and premalignant diseases. The aim of this study was to assess the prognostic value of clinical characteristics to predict treatment outcomes in women with GTD. Materials and Methods: In this retrospective study, 34 patients treated for GTD at the Division of Gynaecology and Perinatology, University Medical Centre Maribor, between 2008 and 2022 were identified. Clinical and pathological characteristics were obtained by analysing patient data records. Results: Within the cohort of 34 patients with GTD, 29 patients (85.3%) had a partial hydatidiform mole (HM) and five patients545 (14.7%) had a complete HM. Two patients with a complete HM developed a postmolar gestational trophoblastic neoplasia (GTN), which represents 5.8% of all cases. Conclusions: GTD is a rare disease that is frequently asymptomatic. The subsequent consequences of GTD, which can lead to malignant transformation, as well life-threatening disease complications, warrant training for early recognition of HMs and timely treatment and surveillance.
Prevalence of Poor Sleep Quality and Associated Factors in Individuals with Rheumatoid Arthritis: A Cross-Sectional Study
Authors: Juárez-Rojop Isela Esther , Fresán Ana , Genis-Mendoza Alma Delia , Cerino-Palomino Carolina , Nolasco-Rosales German Alberto , González-Castro Thelma Beatriz , López-Narváez María Lilia , Olan Francisco , Villar-Soto Mario , Tovilla-Zárate Carlos Alfonso , Nicolini Humberto
Published: September 2023

Background and Objectives: Poor sleep quality has been frequently observed in individuals with rheumatoid arthritis. In the present study, we analyzed the presence of poor sleep quality in a sample of Mexican individuals with rheumatoid arthritis; then, we compared sociodemographic and clinical characteristics among patients to determine risk factors for poor sleep quality. Materials and Methods: In this cross-sectional study, we included 102 individuals with rheumatoid arthritis from a hospital in Mexico. We evaluated disease activity (DAS28), quality of sleep using the Pittsburgh Sleep Quality Index, and the presence of depression and anxiety with the Hospital Anxiety and Depression Scale. We performed a Chi-square test and a t-test. Then, we performed a logistic regressions model of the associated features in a univariable analysis. Results: Poor sleep quality was observed in 41.75% of the individuals with rheumatoid arthritis. Being married was a proactive factor (OR 0.04, 95% CI 0.1–0.9, p = 0.04), whereas having one’s hips affected or presenting with anxiety and depression was associated with poor sleep quality (OR 4.6, 95% CI 1.2–17.69, p = 0.02). After a multivariate analysis, having anxiety (OR 5.0, 95% CI 1.4–17.7, p < 0.01) and depression (OR 9.2, 95% CI 1.0–8.1, p < 0.01) remained associated with a higher risk of having poor sleep quality. Other clinical characteristics among patients were not significantly different. Conclusions: Our results showed that individuals with rheumatoid arthritis who also presented with depression or anxiety had a higher risk of suffering from poor sleep quality. However, more studies with larger samples are necessary to replicate these results in the Mexican population.
Molecular Dynamics Study of Crystallization Behavior in the Solid State of Zr-Cu Amorphous Alloys
Authors: Cao Shuai , Liu Guangyin , Huang Jiankang , Yu Xiaoquan , Luo Yiming , Fan Ding
Published: September 2023

Amorphous alloys show interesting mechanical properties as well as unique physical and chemical properties due to their atomic stacking structure. However, when partial crystallization occurs in amorphous alloys, it can impact the properties of the original amorphous alloy. To study the crystallization phenomenon in the Zr-based amorphous alloy, a three-dimensional Zr-based amorphous alloy atomic-stacking model was established by molecular dynamics simulations, and the atomic structure evolution of the Zr-Cu amorphous system after partial crystallization was analyzed by the radial distribution function g(r), HA bond index and Voronoi polyhedron. The results showed that adding more copper (Cu) atoms to the Zr-Cu amorphous system greatly improves its stability at high temperatures. The atomic diffusion was analyzed by root-mean-square displacement of atoms (MSD); as the temperature rose, the MSD of atoms also increased, suggesting that the crystallization of the amorphous material occurs due to the short-range diffusion of atoms. The analysis of the mechanism of the high-temperature action time on the Zr80Cu20 amorphous alloy showed that the crystallization phase precipitation rate of the amorphous alloy gradually increases with time, but it does not change linearly.
Nonnegligible Temperature Drop Induced by Thermocouple on Samples in Gleeble Tests
Authors: Zhao Xiuhong , Ma Yuting , Gao Shuailong , Li Xuexiong , Yu Dejun , Wang Hao
Published: September 2023

High-temperature plastic deformation is one of the main methods for the fabrication of titanium-based alloys. Accurate determination of the mechanical constitutive relation is pivotal for the design, optimization, as well as the prediction of the mechanical behavior of materials. In this study, finite element simulations were carried out to simulate the Gleeble thermal compression experiment of titanium alloy samples, focusing on different thermocouple design parameters, including thermocouple material and wire diameter, etc. The results show that the heat dissipation of the thermocouple distorted the local temperature field of the contact point between the sample and the thermocouple, resulting in a deviation of the measured temperature. Through finite element method (FEM) simulation and analysis of the changes caused by various factors and comparison with the metallographic morphology of titanium alloy samples from relevant heat treatment experiments, it was shown that the material and wire diameter of the thermocouple, the thermal conductivity coefficient of sample, and the testing temperature of the sample all affected the measurement results. The thermal conductivity of sample had the largest influence on the measurement accuracy. Based on the finite element simulation and experimental comparison, the corresponding correction method and correction formula are proposed.
Optimization of the Fermentation Conditions of Huaniu Apple Cider and Quantification of Volatile Compounds Using HS-SPME-GC/MS
Authors: Mu Yuwen , Zeng Chaozhen , Qiu Ran , Yang Jianbin , Zhang Haiyan , Song Juan , Yuan Jing , Sun Jing , Kang Sanjiang
Published: September 2023

The fermentation process and composition of volatile compounds play a crucial role in the production of Huaniu apple cider. This study aimed to optimize the fermentation conditions of Huaniu apple cider and quantify its volatile compounds using headspace solid-phase microextraction-gas chromatography–mass spectrometry (HS-SPME-GC/MS). The optimal fermentation parameters were determined using response surface methodology (RSM). The optimal fermentation temperature was 25.48 °C, initial soluble solids were 18.90 degrees Brix, inoculation amount was 8.23%, and initial pH was 3.93. The fermentation rate was determined to be 3.0, and the predicted value from the verification test was 3.014. This finding demonstrated the excellent predictability of a RSM-optimized fermentation test for Huaniu apple cider, indicating the reliability of the process conditions. Moreover, the analysis of volatile compounds in the optimized Huaniu cider identified 72 different ingredients, including 41 esters, 16 alcohols, 6 acids, and 9 other substances. Notably, the esters exhibited high levels of ethyl acetate, ethyl octanoate, and ethyl capricate. Similarly, the alcohols demonstrated higher levels of 3-methyl-1-butanol, phenethylethanol, and 2-methyl-1-propanol, while the acids displayed increased concentrations of acetic acid, caproic acid, and caprylic acid. This study provides the essential technical parameters required for the preparation of Huaniu apple cider while also serving as a valuable reference for investigating its distinct flavor profile.
Inducing Rhizosphere Acidification in White Willow with Bacillus sp. ZV6 Enhances Ni Phytoextraction from Soil and Soil Quality
Authors: Virk Zaheer Abbas , Yasin Muhammad Zubair , Gill Sebam , Ilyas Muhammad Fraz , Dradrach Agnieszka , Alamri Saud , Alfagham Alanoud T. , Akhtar Mohd Sayeed , Iqbal Muhammad
Published: September 2023

Chelating agents may decrease the extent of Ni phytoextraction by reducing plant growth and soil health due to Ni toxicity during enhanced phytoextraction. Contrarily, inducing acidity in the rhizosphere of Ni-accumulating plants with plant growth-promoting rhizobacteria (PGPR) having rhizosphere acidification ability can enhance Ni phytoextraction by increasing Ni bioavailability in the soil, plant growth, and plant stress tolerance. We investigated the efficacy of a PGPR species with rhizosphere acidification potential, named Bacillus sp. ZV6 (ARB), in enhancing Ni phytoextraction by white willow (Salix alba) from a Ni-affected soil. The plants were grown for 120 days in soil with zero, threshold, and moderate Ni pollution levels (0, 50, and 100 mg Ni kg−1 soil, respectively) with and without ARB inoculation. After harvest, the effects of the treatments on rhizosphere acidification and associated Ni bioavailability in this zone, Ni distribution in plants, and Ni removal from the soil were investigated. Moreover, enzyme activity, count of bacteria, biomass of microbes, and organic C in the soil, together with indices of plant growth and antioxidant defense, were evaluated. The ARB inoculation significantly improved the plant parameters and soil health and reduced plant oxidative stress at each Ni level compared to the treatments lacking ARB. Besides lowering the soil pH and increasing Ni bioavailability in the rhizosphere with respect to the bulk zone, ARB inoculation exerted additional effects. Surprisingly, the Ni 100 + ARB treatment induced the highest decrease in soil pH (0.32 unit) and an increase in DPTA-extractable Ni (0.45 mg kg−1 soil) between that measured in the bulk zones and that obtained in the rhizosphere zone. Ni distribution in plant parts and Ni removal (% of total Ni) from the soil were also significantly improved with ARB inoculation, compared to the Ni treatments without ARB. The extent of Ni removal was similar for the Ni 50 + ARB (0.27%) and Ni 100 + ARB (0.25%) treatments. Concluding, ARB-inoculated Salix alba can remove similar amounts of Ni from the soil, irrespective of the Ni pollution level.
The Impact of Pulp Temperature on the Flotation Performance of Copper-Lead-Zinc Sulphide Ore
Authors: Bicak Ozlem , Cakir Ertuna , Ozcelik Seda , Ekmekci Zafir
Published: September 2023

Flotation performance can significantly be affected by seasonal variations due to the changes in water temperature and pulp temperature, type and concentration of dissolved ions in process water. Extreme temperature conditions could be the major factor affecting flotation performance and mask the influence of water chemistry. Therefore, the interactive effects of the temperature and water chemistry should be taken into consideration, particularly for mine sites experiencing extreme temperature conditions. In this paper, effects of temperature, sulphate (SO42−), thiosulphate (S2O32−) and calcium (Ca2+) ions on the flotation performance of a Cu-Pb-Zn complex sulfide ore were investigated using a statistical experimental design and modelling approach. The results were evaluated using ANOVA and regression analysis to determine the significant parameters and derive individual regression models for each flotation response using Design Expert software version 6.0.8. Individual regression models were developed for mass pull, water recovery, grade and recovery of the sulfide minerals using the statistically significant main effects and their interactions. The models were used to determine the concentration of the dissolved ions and pulp temperature required to achieve the maximum zinc recovery, maximum zinc grade or the optimum zinc grade and recovery. The results showed that the water chemistry (i.e., the concentrations of Ca2+, SO42− and S2O32−) affected the flotation performance significantly at low temperature (25 °C). At high pulp temperature (60 °C), however, the temperature was the dominant parameter and masked the effects of water chemistry. Details of the statistical experimental design, discussions of the effects of experimental factors and their interactions on flotation performance, and the development of regression models are presented in this paper.