bluuebunny/crossref_metadata_2025 huggingface dataset:
Current Operational Model for Veterinary Care in Large Animal Shelters During Disasters
Authors: Dieckmann Hayley G. , Costa Lais R.R. , Madigan John E.
Published: July 2020

Standardization of operating guidelines for veterinary care in evacuation and emergency animal shelters is imperative for an organized response and to facilitate treatment and prevention of medical conditions stemming from the effects of a disaster and the associated outcomes of sheltering. Safeguarding animal welfare through creating guidelines should expedite care, assure consistency, and provide appropriate safety measures for animals and veterinary medical providers. Veterinary integration into an incident command system (ICS) and response training allows for proper allocation of roles and responsibilities, as well as coordination of veterinary supplies and personnel. Central components of the operational model include guidelines for animal identification, triage, medical records, medical treatments, and discharge instructions. An outline for communication with disaster response agencies, as well as animal owners, is aimed to inform appropriate conduct. Improving the animal component of disaster response is integral in meeting societal needs and improving animal welfare in the face of a disaster.
Developing a Lay First Responder Program in Chad: A 12-Month Follow-Up Evaluation of a Rural Prehospital Emergency Care Program
Authors: Hancock Canaan J. , Delaney Peter G. , Eisner Zachary J. , Kroner Eric , Mahamet-Nuur Issa , Scott John W. , Raghavendran Krishnan
Published: July 2020

Introduction:

The World Health Organization (WHO; Geneva, Switzerland) recommends lay first responder (LFR) programs as a first step toward establishing formal Emergency Medical Services (EMS) in low- and middle-income countries (LMICs) to address injury. There is a scarcity of research investigating LFR program development in predominantly rural settings of LMICs.

Study Objective:

A pilot LFR program was launched and assessed over 12 months to investigate the feasibility of leveraging pre-existing transportation providers to scale up prehospital emergency care in rural, low-resource settings of LMICs.

Methods:

An LFR program was established in rural Chad to evaluate curriculum efficacy, using a validated 15-question pre-/post-test to measure participant knowledge improvement. Pre-/post-test score distributions were compared using a Wilcoxon Signed-Rank test. For test evaluation, each pre-test question was mapped to its corresponding post-test analog and compared using McNemar’s Chi-Squared Test to examine knowledge acquisition on a by-question basis. Longitudinal prehospital care was evaluated with incident reports, while program cost was tracked using a one-way sensitivity analysis. Qualitative follow-up surveys and semi-interviews were conducted at 12 months, with initial participants and randomly sampled motorcycle taxi drivers, and used a constructivist grounded theory approach to understand the factors motivating continued voluntary participation to inform future program continuity. The consolidated criteria for reporting qualitative research (COREQ) checklist was used to guide design, analysis, and reporting the qualitative results.

Results:

A total of 108 motorcycle taxi participants demonstrated significant knowledge improvement (P <.001) across three of four curricular categories: scene safety, airway and breathing, and bleeding control. Lay first responders treated 71 patients over six months, encountering five deaths, and provided patient transport in 82% of encounters. Lay first responders reported an average confidence score of 8.53/10 (n = 38). In qualitative follow-up surveys and semi-structured interviews, the ability to care for the injured, new knowledge/skills, and the resultant gain in social status and customer acquisition motivated continued involvement as LFRs. Ninety-six percent of untrained, randomly sampled motorcycle taxi drivers reported they would be willing to pay to participate in future training courses.

Conclusion:

Lay first responder programs appear feasible and cost-effective in rural LMIC settings. Participants demonstrate significant knowledge acquisition, and after 12 months of providing emergency care, report sustained voluntary participation due to social and financial benefits, suggesting sustainability and scalability of LFR programs in low-resource settings.
Modified Mediterranean diet score adapted to a southern Mediterranean population and its relation to overweight and obesity risk
Authors: El Kinany Khaoula , Mint Sidi Deoula Meimouna , Hatime Zineb , Boudouaya Hanae Abir , Atassi Mariam , El Asri Achraf , Benslimane Abdelilah , Nejjari Chakib , Ibrahimi Sidi Adil , Lagiou Pagona , El Rhazi Karima
Published: July 2020

Objectives:

The current study aimed to develop a modified Mediterranean diet (MMD) score adjusted to the southern Mediterranean countries’ cultural specificities and to evaluate associations between adherence to this modified score and overweight/obesity risk in Moroccan adults.

Design:

Population-based cross-sectional study.

Setting:

Rural and urban areas of the five greatest provinces of Morocco.

Participants:

In total, 1516 participants were recruited between September 2009 and February 2017. Dietary assessment was obtained using a validated Moroccan FFQ. We constructed a MMD score focusing on twelve components. The MMD score ranged from 0 (no adherence to the traditional southern Mediterranean diet (MD)) to 12 (maximal adherence) and was categorised as low (scores 0–4), moderate (scores 5–7) and high (scores 8–12).

Results:

Among the whole population, 754 (50·5 %) were women and 738 (49·5 %) were men, and the mean age was about 55·60 ± 13·70. In total, 58 % of participants were moderately active. Regarding educational level, 50·7 % were illiterate. The prevalence of overweight and obesity was 43·3 and 8·6 %, respectively. In multivariate analyses, close adherence to MMD (scores 8–12) was associated with reduced overweight/obesity risk (OR 0·61, 95 % CI 0·44, 0·84).

Conclusion:

The prevalence of overweight and obesity was very high among Moroccan adults. Adherence to the traditional southern MD may help prevent overweight and obesity.
If I Had Something to Add, I Would
Authors: Lindquist Isaac A. , Adams Emily E. , Allen Joseph A.
Published: July 2020

Abstract. Most employees participate in workplace meetings, and their experiences in meetings can vary greatly, which can lead to positive or negative effects on both job attitudes and behavior. In this study, we examined the effect that a meeting attendee’s competence in the meeting topic had on their participation in the meeting and their perception of meeting effectiveness. Results indicated those with higher levels of competence in the meeting topic were more likely to participate and through participation found their meeting more effective; this relationship was stronger when employee dissent in the meetings was high. Leaders should ensure that those who are present in a meeting are competent in the topic of the meeting and encourage them to participate.
Effect of Dust Load on the Cloud Top Ice‐Water Partitioning Over Northern Middle to High Latitudes With CALIPSO Products
Authors: Kawamoto Kazuaki , Yamauchi Akira , Suzuki Kentaroh , Okamoto Hajime , Li Jiming
Published: July 2020

We quantified effects of dust load on the cloud top ice cloud fraction (ICF) in terms of the dust extinction coefficient (σext). We analyzed 3‐year data sets obtained from an active satellite sensor over middle to high latitudes in the northern hemisphere for temperatures (T) between 230 and 273 K and σext values between 0.005 and 0.145 km−1. At about 250 K, ICF changed by about 30% in response to the above range of σext, whereas at extreme T values, ICF was relatively insensitive to σext. Thus, we concluded that ICF was primarily determined by T, with substantial influence of σext at about 250 K, likely due to increased opportunities for freezing as σext increases. Sensitivity of ICF was the lowest both at the largest σext and lowest T and at the smallest σext and highest T, while it was the highest at about 0.03 km−1 of σext and about 250 K.
CO2‐Brine Substitution Effects on Ultrasonic Wave Propagation Through Sandstone With Oblique Fractures
Authors: Falcon‐Suarez Ismael Himar , Papageorgiou Giorgos , Jin Zhaoyu , Muñoz‐Ibáñez Andrea , Chapman Mark , Best Angus I.
Published: July 2020

Seismic monitoring of injected CO2 plumes in fractured storage reservoirs relies on accurate knowledge of the physical mechanisms governing elastic wave propagation, as described by appropriate, validated rock physics models. We measured laboratory ultrasonic velocity and attenuation of P and S waves, and electrical resistivity, of a synthetic fractured sandstone with obliquely aligned (penny‐shaped) fractures, undergoing a brine‐CO2 flow‐through test at simulated reservoir pressure and temperature. Our results show systematic differences in the dependence of velocity and attenuation on fluid saturation between imbibition and drainage episodes, which we attribute to uniform and patchy fluid distributions, respectively, and the relative permeability of CO2 and brine in the rock. This behavior is consistent with predictions from a multifluid rock physics model, facilitating the identification of the dispersive mechanisms associated with wave‐induced fluid flow in fractured systems at seismic scales.
Exceptional Tropical Cyclone Kenneth in the Far Northern Mozambique Channel and Ocean Eddy Influences
Authors: Mawren D. , Hermes J. , Reason C. J. C.
Published: July 2020

During summer 2018/2019, Mozambique was devastated by Idai and Kenneth, the first time two Intense Status tropical cyclones have ever made landfall here. The season broke numerous records for the Southwest Indian Ocean (the greatest deaths, the largest damage, and numbers of Intense Status cyclones and tropical storms). Kenneth, which is the strongest cyclone ever to make landfall in Mozambique, did so unusually late in the season, had an unusual due westward track and struck the coast far further north than previously, is examined. Changes in Kenneth's strength (21–25 April 2019) across the extreme northern Mozambique Channel are shown to be related to two cyclonic/anticyclonic eddy pairs in the ocean. These eddies led to changes in SST, enthalpy fluxes, tropical cyclone heat potential, and upper ocean heat content that influenced its rapid strengthening from moderate tropical storm to Very Intense tropical cyclone status and then back to Intense Status just before landfall.
High Sensitivity of Arctic Black Carbon Radiative Effects to Subgrid Vertical Velocity in Aerosol Activation
Authors: Matsui Hitoshi , Moteki Nobuhiro
Published: July 2020

The representation of aerosol activation into cloud droplets in climate models is important for accurate understanding of aerosol radiative impacts on the Arctic climate, but it remains highly uncertain. Here we show that the uncertainty range of subgrid vertical velocity (SVV) and maximum supersaturation (SSmax) in aerosol activation produces fourfold to fivefold differences in the direct radiative effect of black carbon (BC) in the Arctic (0.091–0.40 W m−2) because SVV and SSmax determine the activated fraction and wet removal efficiency of aerosols. Aerosols are particularly sensitive to SVV in remote regions but not near their sources because many aerosols near sources are not yet influenced by wet removal processes. Our results demonstrate that SVV treatment is a major source of uncertainty in Arctic aerosol simulations and may be key for reducing the large discrepancies among global models in estimates of BC and its radiative effects in the Arctic.
Hydrologic Implications of Wind Farm Effect on Radar‐Rainfall Observations
Authors: Ghimire Ganesh R. , Krajewski Witold F.
Published: July 2020

Despite efforts to detect and mitigate wind farm clutter in weather radar observations of rainfall, these signatures propagate to quantitative precipitation estimates. In this study, the authors investigate the hydrologic impact of wind farm clutter in the Multi‐Radar Multi‐Sensor rainfall products. The study uses the probability of detection method to identify wind farm clutter in data from Iowa for the years 2016 and 2017. Using the physically based distributed hydrologic model called the Hillslope‐Link Model, the authors show that streamflow (flood) prediction errors are generally significant at smaller basin communities where wind farms occupy a large portion of the upstream basins. These errors due to wind farm clutter show systematic decrease with the basin scales. The results from this study have implications for real‐time streamflow forecasts provided automatically by the National Water Model at the National Oceanic and Atmospheric Administration, particularly at small riverine communities.
Impaired AKT signaling and lung tumorigenesis by PIERCE1 ablation in KRAS-mutant non-small cell lung cancer
Authors: Roh Jae-il , Lee Jaehoon , Sung Young-Hoon , Oh Jahyun , Hyeon Do Young , Kim Yujin , Lee Seungeon , Devkota Sushil , Kim Hye Jeong , Park Bomin , Nam Taewook , Song Yaechan , Kim Yonghwan , Hwang Daehee , Lee Han-Woong
Published: July 2020

KRAS-mutant non-small cell lung cancer (NSCLC) is a major lung cancer subtype that leads to many cancer-related deaths worldwide. Although numerous studies on KRAS-mutant type NSCLC have been conducted, new oncogenic or tumor suppressive genes need to be detected because a large proportion of NSCLC patients does not respond to currently used therapeutics. Here, we show the tumor-promoting function of a cell cycle-related protein, PIERCE1, in KRAS-mutant NSCLC. Mechanistically, PIERCE1 depletion inhibits cell growth and AKT phosphorylation (pAKT) at S473, which is particularly observed in KRAS-mutant lung cancers. Analyses of AKT-related genes using microarray, immunoblotting, and real-time quantitative PCR indicated that PIERCE1 negatively regulates the gene expression of the AKT suppressor, TRIB3, through the CHOP pathway, which is a key regulatory pathway for TRIB3 expression. Similarly, in vivo analyses of PIERCE1 depletion in the KRAS mutation-related lung cancer mouse models revealed the suppressive effect of PIERCE1 knockout in urethane- and KRASG12D-induced lung tumorigenesis with decreased pAKT levels observed in the tumors. Tissue microarrays of human lung cancers indicated the expression of PIERCE1 in 83% of lung cancers and its correlation with pAKT expression. Thus, we illustrate how PIERCE1 depletion may serve as a therapeutic strategy against KRAS-mutant NSCLC and propose the clinical benefit of PIERCE1.
The identification of nuclear αvβ3 integrin in ovarian cancer: non-paradigmal localization with cancer promoting actions
Authors: Seraya-Bareket Chen , Weisz Avivit , Shinderman-Maman Elena , Teper-Roth Sharon , Stamler Dina , Arbib Nissim , Kadan Yfat , Fishman Ami , Kidron Debora , Edelstein Evgeny , Ellis Martin , Ashur-Fabian Osnat
Published: July 2020

Nuclear translocation of transmembrane proteins was reported in high-grade serous ovarian cancer (HGSOC), a highly aggressive gynecological malignancy. Although the membrane receptor αvβ3 integrin is amply expressed in HGSOC and involved in disease progression, its nuclear localization was never demonstrated. Nuclear αvβ3 was explored in HGSOC cells (OVCAR3, KURAMOCHI, and JHOS4), nuclear localization signal (NLS) modified β3 OVCAR3, Chinese hamster ovaries (CHO-K1) and human embryonic kidney (HEK293) before/after transfections with β3/β1 integrins. We used the ImageStream technology, Western blots (WB), co immunoprecipitations (Co-IP), confocal immunofluorescence (IF) microscopy, flow cytometry for cell counts and cell cycle, wound healing assays and proteomics analyses. Fresh/archived tumor tissues were collected from nine HGSOC patients and normal ovarian and fallopian tube (FT) tissues from eight nononcological patients and assessed for nuclear αvβ3 by WB, confocal IF microscopy and immunohistochemistry (IHC). We identified nuclear αvβ3 in HGSOC cells and tissues, but not in normal ovaries and FTs. The nuclear integrin was Tyr 759 phosphorylated and functionally active. Nuclear αvβ3 enriched OVCAR3 cells demonstrated induced proliferation and oncogenic signaling, intact colony formation ability and inhibited migration. Proteomics analyses revealed a network of nuclear αvβ3-bound proteins, many of which with key cancer-relevant activities. Identification of atypical nuclear localization of the αvβ3 integrin in HGSOC challenges the prevalent conception that the setting in which this receptor exerts its pleiotropic actions is exclusively at the cell membrane. This discovery proposes αvβ3 moonlighting functions and may improve our understanding of the molecular basis of ovarian cancer pathogenesis.
Targeted therapies in gynecological cancers: a comprehensive review of clinical evidence
Authors: Wang Qiao , Peng Hongling , Qi Xiaorong , Wu Min , Zhao Xia
Published: July 2020

Advanced and recurrent gynecological cancers are associated with poor prognosis and lack of effective treatment. The developments of the molecular mechanisms on cancer progression provide insight into novel targeted therapies, which are emerging as groundbreaking and promising cancer treatment strategies. In gynecologic malignancies, potential therapeutic targeted agents include antiangiogenic agents, poly (ADP-ribose) polymerase (PARP) inhibitors, tumor-intrinsic signaling pathway inhibitors, selective estrogen receptor downregulators, and immune checkpoint inhibitors. In this article, we provide a comprehensive review of the clinical evidence of targeted agents in gynecological cancers and discuss the future implication.
New insights into the evasion of host innate immunity by Mycobacterium tuberculosis
Authors: Chai Qiyao , Wang Lin , Liu Cui Hua , Ge Baoxue
Published: July 2020

Mycobacterium tuberculosis (Mtb) is an extremely successful intracellular pathogen that causes tuberculosis (TB), which remains the leading infectious cause of human death. The early interactions between Mtb and the host innate immune system largely determine the establishment of TB infection and disease development. Upon infection, host cells detect Mtb through a set of innate immune receptors and launch a range of cellular innate immune events. However, these innate defense mechanisms are extensively modulated by Mtb to avoid host immune clearance. In this review, we describe the emerging role of cytosolic nucleic acid-sensing pathways at the host–Mtb interface and summarize recently revealed mechanisms by which Mtb circumvents host cellular innate immune strategies such as membrane trafficking and integrity, cell death and autophagy. In addition, we discuss the newly elucidated strategies by which Mtb manipulates the host molecular regulatory machinery of innate immunity, including the intranuclear regulatory machinery, the ubiquitin system, and cellular intrinsic immune components. A better understanding of innate immune evasion mechanisms adopted by Mtb will provide new insights into TB pathogenesis and contribute to the development of more effective TB vaccines and therapies.
Index and biological spectrum of human DNase I hypersensitive sites
Authors: Meuleman Wouter , Muratov Alexander , Rynes Eric , Halow Jessica , Lee Kristen , Bates Daniel , Diegel Morgan , Dunn Douglas , Neri Fidencio , Teodosiadis Athanasios , Reynolds Alex , Haugen Eric , Nelson Jemma , Johnson Audra , Frerker Mark , Buckley Michael , Sandstrom Richard , Vierstra Jeff , Kaul Rajinder , Stamatoyannopoulos John
Published: July 2020

DNase I hypersensitive sites (DHSs) are generic markers of regulatory DNA1–5 and contain genetic variations associated with diseases and phenotypic traits6–8. We created high-resolution maps of DHSs from 733 human biosamples encompassing 438 cell and tissue types and states, and integrated these to delineate and numerically index approximately 3.6 million DHSs within the human genome sequence, providing a common coordinate system for regulatory DNA. Here we show that these maps highly resolve the cis-regulatory compartment of the human genome, which encodes unexpectedly diverse cell- and tissue-selective regulatory programs at very high density. These programs can be captured comprehensively by a simple vocabulary that enables the assignment to each DHS of a regulatory barcode that encapsulates its tissue manifestations, and global annotation of protein-coding and non-coding RNA genes in a manner orthogonal to gene expression. Finally, we show that sharply resolved DHSs markedly enhance the genetic association and heritability signals of diseases and traits. Rather than being confined to a small number of distal elements or promoters, we find that genetic signals converge on congruently regulated sets of DHSs that decorate entire gene bodies. Together, our results create a universal, extensible coordinate system and vocabulary for human regulatory DNA marked by DHSs, and provide a new global perspective on the architecture of human gene regulation.
Detrimental effects of hypercortisolism on brain structure and related risk factors
Authors: Chen Yaxi , Zhang Junhuai , Tan Huiwen , Li Jiaqi , Yu Yerong
Published: July 2020

Brain structural abnormalities are often observed on magnetic resonance imaging (MRI) scans of Cushing's syndrome patients, but the pathogenesis is not fully understood. To understand the relationship between brain structural abnormalities and potential risk factors in active Cushing's disease (CD) patients, a total of 101 treatment-naïve CD patients and 95 sex-, age- and education matched controls with non-functioning adenomas (NFA) underwent clinical evaluation and MRI investigation, and the relative risk factors were analyzed. 14 patients in sustained remission after transsphenoidal surgery were followed. Compared with the NFA subjects, the patients with CD had more cortical (P < 0.01) and subcortical atrophy (P < 0.01) and a higher prevalence of white matter hyperintensity (WMH) (P < 0.01). WMH severity in CD patients positively correlated with age (r = 0.532, P = 0.000), disease course (r = 0.257, P = 0.009), postprandial glucose (r = 0.278, P = 0.005), frequency of left ventricular hypertrophy (r = 0.398, P = 0.001) and hypothyroidism (r = 0.246, P = 0.014). The markers of cortical and subcortical atrophy (sylvian fissure ratio, bifrontal ratio, bicaudate ratio and third ventricle width) were positively associated with the progression of WMH in the CD patients. In the follow-up of 14 patients with CD, brain atrophy and WMH was partially reversible after correction of hypercortisolism. In conclusions, brain atrophy and WMH were more likely to appear in CD patients and were possibly partially reversible following correction of hypercortisolism.
Individual-based population genomics reveal different drivers of adaptation in sympatric fish
Authors: Torrado Héctor , Carreras Carlos , Raventos Núria , Macpherson Enrique , Pascual Marta
Published: July 2020

Connectivity and local adaptation are two contrasting evolutionary forces highly influencing population structure. To evaluate the impact of early-life traits and environmental conditions on genetic structuring and adaptation, we studied two sympatric fish species in the Western Mediterranean Sea: Symphodus tinca and S. ocellatus. We followed an individual-based approach and measured early-life history traits from otolith readings, gathered information on environmental variables and obtained genome-wide markers from genotyping-by-sequencing (GBS). The two species presented contrasting population structure across the same geographic gradient, with high and significant population differentiation in S. ocellatus, mostly determined by oceanographic fronts, and low differentiation and no front effect in S. tinca. Despite their different levels of genetic differentiation, we identified in both species candidate regions for local adaptation by combining outlier analysis with environmental and phenotypic association analyses. Most candidate loci were associated to temperature and productivity in S. ocellatus and to temperature and turbulence in S. tinca suggesting that different drivers may determine genomic diversity and differentiation in each species. Globally, our study highlights that individual-based approach combining genomic, environmental and phenotypic information is key to identify signals of selection and the processes mediating them.
Exclusive use of digital PCR allows an absolute assay of heat-killed Lactobacilli in foods targeting multiple copies of 16S rDNA
Authors: Soejima Takashi , Tanaka Miyuki , Yamauchi Koji , Abe Fumiaki
Published: July 2020

The real-time PCR (qPCR) and digital PCR (dPCR) to amplify a single-copy of house-keeping genes (i.e.,hsp60, pheSortuf) are used for the assay of limited microbial species. In general, with a single-copy gene, there are obviously varied DNA sequences for even the same microbial species, which could cause difficulties with design of primers and probes for PCR when targeting various single copy genes. In general, for identification by dPCR (as a representative case:Lactobacillus paracasei), accumulated DNA sequence information of 16S rDNA, which is much more frequently used, should be targeted. In contrast, next-generation sequencing revealed that there are five copies of 16S rDNA in a liveL. paracaseiMCC1849. Therefore, we aimed to reveal, if heat-killedL. paracaseisupplemented in nutritional foods that aid the host immune system have the relevant five copies per chromosomal DNA, and if the relevant copies remain unchanged on the same chromosomal DNA or remain to be different chromosomal DNA fragments. So, we revealed the actual distribution of the potential original five copies of 16S rDNA using our innovative dPCR, in which both 16S rDNA andhsp60genes were simultaneously elongated. The molecular ratios of 16S rDNA/hsp60dispersed in the dPCR chip were then estimated. The 16S rDNA/hsp60molecular ratios of the heat-killedL. paracaseiin foods, resultantly ranged from 5.0 to 7.2, being the same or higher than that of the five copies determined by next-generation sequencing. The 16S rDNA copy number/ratio indicated the chromosomal DNA molecular number and the associated cell number. As significance, different nutritional foods could potentially cause the loss of chromosomal DNA of supplemented beneficial microbes to a much greater degree. Our absolute dPCR does not require standard correlative samples for the estimation of final products. The estimation principle of the ratio of 16S rDNA/a house-keeping single-copy gene by our absolute dPCR could lead to a useful and accurate assay for various nutritional foods.
PPARG (Pro12Ala) genetic variant and risk of T2DM: a systematic review and meta-analysis
Authors: Sarhangi Negar , Sharifi Farshad , Hashemian Leila , Hassani Doabsari Maryam , Heshmatzad Katayoun , Rahbaran Marzieh , Jamaldini Seyed Hamid , Aghaei Meybodi Hamid Reza , Hasanzad Mandana
Published: July 2020

Type 2 diabetes mellitus (T2DM) is a complex disease caused by the interaction between genetic and environmental factors. A growing number of evidence suggests that the peroxisome proliferator-activated receptor gamma (PPARG) gene plays a major role in T2DM development. Meta-analysis of genetic association studies is an efficient tool to gain a better understanding of multifactorial diseases and potentially to provide valuable insights into gene-disease interactions. The present study was focused on assessing the association between Pro12Ala variation in the PPARG and T2DM risk through a comprehensive meta-analysis. We searched PubMed, WoS, Embase, Scopus and ProQuest from 1990 to 2017. The fixed-effect or random-effect model was used to evaluate the pooled odds ratios (ORs) and 95% confidence intervals (CIs) depending on the heterogeneity among studies. The sources of heterogeneity and publication bias among the included studies were assessed using I2 statistics and Egger's tests. A total of 73 studies, involving 62,250 cases and 69,613 controls were included. The results showed that the minor allele (G) of the rs1801282 variant was associated with the decreased risk of T2DM under different genetic models. Moreover, the protective effect of minor allele was detected to be significantly more in some ethnicities including the European (18%), East Asian (20%), and South East Asian (18%). And the reduction of T2DM risk in Ala12 carriers was stronger in individuals from North Europe rather than Central and South Europe. Our findings indicated that the rs1801282 variant may contribute to decrease of T2DM susceptibility in different ancestries.
Nanoparticle size distribution from inversion of wide angle X-ray total scattering data
Authors: Ferri Fabio , Bertolotti Federica , Guagliardi Antonietta , Masciocchi Norberto
Published: July 2020

An increasingly important issue in nanoscience and nanotechnology is the accurate determination of nanoparticle sizing. Wide angle X-ray total scattering (WAXTS) data are frequently used to retrieve the Particle Size Distributions (PSDs) of nanocrystals of highly technological relevance; however, the PSD shape typically relies on an a-priori assumption. Here, we propose a modified version of the classical iterative Lucy-Richardson (LR) algorithm, which is simple, fast and highly reliable against noise, and demonstrate that the inversion of WAXTS data can be profitably used for recovering accurate PSD regardless of its shape. Computer simulations based on the use of the Debye Scattering Equation (DSE) modelling WAXTS data show that the algorithm is capable of recovering accurate PSDs even when the sample is made of a mixture of different polymorphs and/or exhibits microstrain effects. When applied to the inversion of WAXTS data taken on real samples, the method requires accurate modelling of the nanoparticle crystal structure, which includes structural defects, microstrain and surface induced distortions. Provided that this information is correctly fed to the program, the inversion algorithm reconstructs the WAXTS data with high accuracy and recovers highly robust (against noise) PSDs. Two examples reporting the characterization of Magnetite-Maghemite and commercial P25-Titania nanopowders, are discussed. We demonstrate that pre-assumption of wrong PSD shape leads to inaccurate number-based average sizes in highly polydisperse samples.
Against all odds: a tale of marine range expansion with maintenance of extremely high genetic diversity
Authors: Robalo Joana I. , Francisco Sara M. , Vendrell Catarina , Lima Cristina S. , Pereira Ana , Brunner Benedikt P. , Dia Mamadou , Gordo Leonel , Castilho Rita
Published: July 2020

The displacement of species from equatorial latitudes to temperate locations following the increase in sea surface temperatures is among the significant reported consequences of climate change. Shifts in the distributional ranges of species result in fish communities tropicalisation, i.e., high latitude colonisations by typically low latitude distribution species. These movements create new interactions between species and new trophic assemblages. The Senegal seabream,Diplodus bellottii, may be used as a model to understand the population genetics of these invasions. In the last decades, this species has undergone an outstanding range expansion from its African area of origin to the Atlantic coast of the Iberian Peninsula, where now occurs abundantly. Mitochondrial and nuclear markers revealed a striking high haplotypic nucleotide and genetic diversity values, along with significant population differentiation throughout the present-day geographical range of the Senegal seabream. These results are not consistent with the central-marginal hypothesis, nor with the expectations of a leptokurtic distribution of individuals, asD. bellottiiseems to be able to retain exceptional levels of diversity in marginal and recently colonised areas. We discuss possible causes for hyperdiversity and lack of geographical structure and subsequent implications for fisheries.
Correlation among photoluminescence and the electronic and atomic structures of Sr2SiO4:xEu3+ phosphors: X-ray absorption and emission studies
Authors: Zheng Shi-Yan , Chiou Jau-Wern , Li Yueh-Han , Yang Cheng-Fu , Ray Sekhar Chandra , Chen Kuan-Hung , Chang Chun-Yu , Shelke Abhijeet R. , Wang Hsiao-Tsu , Yeh Ping-Hung , Lai Chun-Yen , Hsieh Shang-Hsien , Pao Chih-Wen , Chen Jeng-Lung , Lee Jyh-Fu , Tsai Huang-Ming , Fu Huang-Wen , Hua Chih-Yu , Lin Hong-Ji , Chen Chien-Te , Pong Way-Faung
Published: July 2020

A series of Eu3+-activated strontium silicate phosphors, Sr2SiO4:xEu3+ (SSO:xEu3+, x = 1.0, 2.0 and 5.0%), were synthesized by a sol–gel method, and their crystalline structures, photoluminescence (PL) behaviors, electronic/atomic structures and bandgap properties were studied. The correlation among these characteristics was further established. X-ray powder diffraction analysis revealed the formation of mixed orthorhombic α'-SSO and monoclinic β-SSO phases of the SSO:xEu3+ phosphors. When SSO:xEu3+ phosphors are excited under ultraviolet (UV) light (λ = 250 nm, ~ 4.96 eV), they emit yellow (~ 590 nm), orange (~ 613 nm) and red (~ 652 and 703 nm) PL bands. These PL emissions typically correspond to 4f–4f electronic transitions that involve the multiple excited 5D0 → 7FJ levels (J = 1, 2, 3 and 4) of Eu3+ activators in the host matrix. This mechanism of PL in the SSO:xEu3+ phosphors is strongly related to the local electronic/atomic structures of the Eu3+–O2− associations and the bandgap of the host lattice, as verified by Sr K-edge and Eu L3-edge X-ray absorption near-edge structure (XANES)/extended X-ray absorption fine structure, O K-edge XANES and Kα X-ray emission spectroscopy. In the synthesis of SSO:xEu3+ phosphors, interstitial Eu2O3-like structures are observed in the host matrix that act as donors, providing electrons that are nonradiatively transferred from the Eu 5d and/or O 2p–Eu 4f/5d states (mostly the O 2p–Eu 5d states) to the 5D0 levels, facilitating the recombination of electrons that have transitioned from the 5D0 level to the 7FJ level in the bandgap. This mechanism is primarily responsible for the enhancement of PL emissions in the SSO:xEu3+ phosphors. This PL-related behavior indicates that SSO:xEu3+ phosphors are good light-conversion phosphor candidates for use in near-UV chips and can be very effective in UV-based light-emitting diodes.
Mandibular sawing in a snail-eating snake
Authors: Kojima Yosuke , Fukuyama Ibuki , Kurita Takaki , Hossman Mohamad Yazid Bin , Nishikawa Kanto
Published: July 2020

The jaws of vertebrates display a striking diversity in form and function, but they typically open and close like a trapdoor rather than sliding like a saw. Here, we report unique feeding behaviour in the blunt-headed snail-eating snake, Aplopeltura boa (family Pareidae), where the snake cuts off and circumvents the indigestible part (the operculum) of its prey in the mouth using long sliding excursions of one side of the mandible, while the upper jaws and the mandible on the other side maintain a stable grasp on the prey. This behaviour, which we call ‘mandibular sawing’, is made possible by extraordinarily independent movements of the jaw elements and is a surprising departure from usual feeding behaviour in vertebrates.
Transcriptomic profiling of the medicinal plant Clitoria ternatea: identification of potential genes in cyclotide biosynthesis
Authors: Kalmankar Neha V. , Venkatesan Radhika , Balaram Padmanabhan , Sowdhamini Ramanathan
Published: July 2020

Clitoria ternatea a perennial climber of the Fabaceae family, is well known for its agricultural and medical applications. It is also currently the only known member of the Fabaceae family that produces abundant amounts of the ultra-stable macrocyclic peptides, cyclotides, across all tissues. Cyclotides are a class of gene-encoded, disulphide-rich, macrocyclic peptides (26–37 residues) acting as defensive metabolites in several plant species. Previous transcriptomic studies have demonstrated the genetic origin of cyclotides from the Fabaceae plant family to be embedded in the albumin-1 genes, unlike its counterparts in other plant families. However, the complete mechanism of its biosynthesis and the repertoire of enzymes involved in cyclotide folding and processing remains to be understood. In this study, using RNA-Seq data and de novo transcriptome assembly of Clitoria ternatea, we have identified 71 precursor genes of cyclotides. Out of 71 unique cyclotide precursor genes obtained, 51 sequences display unique cyclotide domains, of which 26 are novel cyclotide sequences, arising from four individual tissues. MALDI-TOF mass spectrometry analysis of fractions from different tissue extracts, coupled with precursor protein sequences obtained from transcriptomic data, established the cyclotide diversity in this plant species. Special focus in this study has also been on identifying possible enzymes responsible for proper folding and processing of cyclotides in the cell. Transcriptomic mining for oxidative folding enzymes such as protein-disulphide isomerases (PDI), ER oxidoreductin-1 (ERO1) and peptidylprolyl cis-trans isomerases (PPIases)/cyclophilins, and their levels of expression are also reported. In particular, it was observed that the CtPDI genes formed plant-specific clusters among PDI genes as compared to those from other plant species. Collectively, this work provides insights into the biogenesis of the medicinally important cyclotides and establishes the expression of certain key enzymes participating in peptide biosynthesis. Also, several novel cyclotide sequences are reported and precursor sequences are analysed in detail. In the absence of a published reference genome, a comprehensive transcriptomics approach was adopted to provide an overview of diverse properties and constituents of C. ternatea.
Development of the FAST-DOSE assay system for high-throughput biodosimetry and radiation triage
Authors: Wang Qi , Lee Younghyun , Shuryak Igor , Pujol Canadell Monica , Taveras Maria , Perrier Jay R. , Bacon Bezalel A. , Rodrigues Matthew A. , Kowalski Richard , Capaccio Christopher , Brenner David J. , Turner Helen C.
Published: July 2020

Following a large-scale radiological incident, there is a need for FDA-approved biodosimetry devices and biomarkers with the ability to rapidly determine past radiation exposure with sufficient accuracy for early population triage and medical management. Towards this goal, we have developed FAST-DOSE (Fluorescent Automated Screening Tool for Dosimetry), an immunofluorescent, biomarker-based system designed to reconstruct absorbed radiation dose in peripheral blood samples collected from potentially exposed individuals. The objective of this study was to examine the performance of the FAST-DOSE assay system to quantify intracellular protein changes in blood leukocytes for early biodosimetry triage from humanized NOD-scid-gamma (Hu-NSG) mice and non-human primates (NHPs) exposed to ionizing radiation up to 8 days after radiation exposure. In the Hu-NSG mice studies, the FAST-DOSE biomarker panel was able to generate delivered dose estimates at days 1, 2 and 3 post exposure, whereas in the NHP studies, the biomarker panel was able to successfully classify samples by dose categories below or above 2 Gy up to 8 days after total body exposure. These results suggest that the FAST-DOSE bioassay has large potential as a useful diagnostic tool for rapid and reliable screening of potentially exposed individuals to aid early triage decisions within the first week post-exposure.
A non-invasive soil-based setup to study tomato root volatiles released by healthy and infected roots
Authors: Gulati Sneha , Ballhausen Max-Bernhard , Kulkarni Purva , Grosch Rita , Garbeva Paolina
Published: July 2020

The role of root exudates in mediating plant–microbe interactions has been well documented. However, the function of volatile organic compounds (VOCs) emitted by plant roots has only recently begun to attract attention. This newly recognized relevance of belowground VOCs has so far mostly been tested using systems limited to a two-compartment Petri-dish design. Furthermore, many of the plant–microbe interaction studies have only investigated the effects of microbial VOCs on plant growth. Here, we go two steps further. First we investigated the volatile profile of healthy and pathogen (Fusarium oxysporum) infected tomato roots grown in soil. We then used a unique soil-based olfactometer-choice assay to compare the migration pattern of four beneficial bacteria (Bacillus spp.) towards the roots of the tomato plants. We demonstrate that the blend of root-emitted VOCs differs between healthy and diseased plants. Our results show that VOCs are involved in attracting bacteria to plant roots.
Association between inflammatory bowel disease and bullous pemphigoid: a population-based case–control study
Authors: Chen Yi-Ju , Juan Chao-Kuei , Chang Yun-Ting , Wu Chun-Ying , Ho Hsiu J. , Tseng Hsiao-Ching
Published: July 2020

The coexistence of inflammatory bowel disease (IBD) and bullous pemphigoid (BP) has been reported. No large-scale study to date has explored the relationship between these diseases. This population-based case-control study examined the association between IBD and BP by using a nationwide database. A total of 5,263 BP patients and 21,052 age- and gender-, hospital visit number-matched controls were identified in the National Health Insurance Research Database of Taiwan (1997–2013). Demographic characteristics and comorbidities including IBD were compared. Logistic regression was conducted to examine the predicting factors for BP. The mean age at diagnosis was 74.88 years and 54.3% of subjects were male. BP patients tended to have more cardiovascular risk factors, autoimmune and neurologic comorbidities, and hematologic cancers than matched controls. There were 20 cases of IBD (0.38%), mostly ulcerative colitis (N = 17, 0.32%) among BP patients, compared to 33 IBD cases (0.16%) among controls (p < 0.001). Ulcerative colitis was found to be significantly associated with BP [adjusted odds ratio (OR) 3.60, 95% confidence interval (CI) 1.91–6.77, p < 0.001] on multivariate analysis. Treatment for IBD was not associated with BP development. Information about diet, lifestyle, alcohol consumption, and smoking habit was not available. We concluded that UC is independently associated with BP.
Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace
Authors: Chessa Daniela , Murgia Manuela , Sias Emanuela , Deligios Massimo , Mazzarello Vittorio , Fiamma Maura , Rovina Daniela , Carenti Gabriele , Ganau Giulia , Pintore Elisabetta , Fiori Mauro , Kay Gemma L. , Ponzeletti Alessandro , Cappuccinelli Piero , Kelvin David J. , Wain John , Rubino Salvatore
Published: July 2020

This study evidenced the presence of parasites in a cesspit of an aristocratic palace of nineteenth century in Sardinia (Italy) by the use of classical paleoparasitological techniques coupled with next-generation sequencing. Parasite eggs identified by microscopy included helminth genera pathogenic for humans and animals: the whipworm Trichuris sp., the roundworm Ascaris sp., the flatworm Dicrocoelium sp. and the fish tapeworm Diphyllobothrium sp. In addition, 18S rRNA metabarcoding and metagenomic sequencing analysis allowed the first description in Sardinia of aDNA of the human specific T. trichiura species and Ascaris genus. Their presence is important for understanding the health conditions, hygiene habits, agricultural practices and the diet of the local inhabitants in the period under study.
Characterization of highly pathogenic avian influenza H5Nx viruses in the ferret model
Authors: Pulit-Penaloza Joanna A. , Brock Nicole , Pappas Claudia , Sun Xiangjie , Belser Jessica A. , Zeng Hui , Tumpey Terrence M. , Maines Taronna R.
Published: July 2020

Highly pathogenic avian influenza (HPAI) H5 viruses, of the A/goose/Guangdong/1/1996 lineage, have exhibited substantial geographic spread worldwide since the first detection of H5N1 virus in 1996. Accumulation of mutations in the HA gene has resulted in several phylogenetic clades, while reassortment with other avian influenza viruses has led to the emergence of new virus subtypes (H5Nx), notably H5N2, H5N6, and H5N8. H5Nx viruses represent a threat to both the poultry industry and human health and can cause lethal human disease following virus exposure. Here, HPAI H5N6 and H5N2 viruses (isolated between 2014 and 2017) of the 2.3.4.4 clade were assessed for their capacity to replicate in human respiratory tract cells, and to cause disease and transmit in the ferret model. All H5N6 viruses possessed increased virulence in ferrets compared to the H5N2 virus; however, pathogenicity profiles varied among the H5N6 viruses tested, from mild infection with sporadic virus dissemination beyond the respiratory tract, to severe disease with fatal outcome. Limited transmission between co-housed ferrets was observed with the H5N6 viruses but not with the H5N2 virus. In vitro evaluation of H5Nx virus replication in Calu-3 cells and the identification of mammalian adaptation markers in key genes associated with pathogenesis supports these findings.
Geographic variation in cardiometabolic risk factor prevalence explained by area-level disadvantage in the Illawarra-Shoalhaven region of the NSW, Australia
Authors: Toms Renin , Mayne Darren J. , Feng Xiaoqi , Bonney Andrew
Published: July 2020

Cardiometabolic risk factors (CMRFs) demonstrate significant geographic variation in their distribution. The study aims to quantify the general contextual effect of the areas on CMRFs; and the geographic variation explained by area-level socioeconomic disadvantage. A cross sectional design and multilevel logistic regression methods were adopted. Data included objectively measured routine pathology test data between years 2012 and 2017 on: fasting blood sugar level; glycated haemoglobin; total cholesterol; high density lipoprotein; urinary albumin creatinine ratio; estimated glomerular filtration rate; and body mass index. The 2011 Australian census based Index of Relative Socioeconomic Disadvantage (IRSD) were the area-level study variables, analysed at its smallest geographic unit of reporting. A total of 1,132,029 CMRF test results from 256,525 individuals were analysed. After adjusting for individual-level covariates, all CMRFs significantly associated with IRSD and the probability of higher risk CMRFs increases with greater area-level disadvantage. Though the specific contribution of IRSD in the geographic variation of CMRF ranged between 57.8 and 14.71%, the general contextual effect of areas were found minimal (ICCs 0.6–3.4%). The results support universal interventions proportional to the need and disadvantage level of populations for the prevention and control of CMRFs, rather than any area specific interventions as the contextual effects were found minimal in the study region.
Evidence for long-term seamount-induced chlorophyll enhancements
Authors: Leitner Astrid B. , Neuheimer Anna B. , Drazen Jeffrey C.
Published: July 2020

Seamounts are ubiquitous global features often characterized by biological hotspots of diversity, biomass, and abundance, though the mechanisms responsible are poorly understood. One controversial explanation suggests seamount-induced chlorophyll enhancements (SICE) subsidize seamount ecosystems. Using a decade of satellite chlorophyll data, we report substantial long-term chlorophyll enhancements around 17% of Pacific seamounts and 45% of shallow (< 100 m) seamounts, with the highest probability of detection at shallow, low-latitude seamounts. SICE is shown to enhance chlorophyll concentrations by up to 56% relative to oceanic conditions, and SICE seamounts have two-fold higher fisheries catch relative to non-enhancing seamounts. Therefore, seamount-induced bottom-up trophic subsidies are not rare, occurring most often at shallow, heavily exploited seamounts, suggesting an important subset of seamounts experience fundamentally different trophic dynamics than previously thought.