bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Intrathecal Baclofen Bolus Injection on Ankle Muscle Activation During Gait in Patients With Acquired Brain Injury
Authors: Chow John W. , Yablon Stuart A. , Stokic Dobrivoje S.
Published: May 2014

Background. Intrathecal baclofen (ITB) bolus injection effectively decreases spinal excitability but the impact on lower limb muscle activation during gait has not been thoroughly investigated. Objective. Examine activation of medial gastrocnemius (MG) and tibialis anterior (TA) muscles during gait before and after ITB bolus injection in patients with resting hypertonia after acquired brain injury. Methods. Lower extremity Ashworth score, temporospatial gait parameters, characteristics of the linear relationship between electromyogram (EMG) and lengthening velocity (LV) in MG during stance, and the duration and magnitude of TA-MG coactivation were assessed before and at 2, 4, and 6 hours after a 50-µg ITB injection via lumbar puncture in 8 hemorrhagic stroke and 11 traumatic brain injury subjects. Results. Temporospatial gait parameters did not significantly differ across the evaluation points ( P ≥ .170). However, Ashworth score ( P < .001), frequency and gain of significant positive EMG-LV slope ( P ≤ .020), and duration of TA-MG coactivation ( P ≤ .013) significantly decreased in the more-affected leg after ITB bolus. EMG changes were not significantly different between patients who did (n = 10) and did not (n = 9) increase gait speed after the injection. The timing of the largest decrease in Ashworth score and the largest decrease in EMG parameters coincided in 36% of cases, on average. Conclusions. ITB bolus injection alters the activation of MG and TA during gait. However, the changes in muscle activation are not closely related to the changes in gait speed or resting muscle hypertonia. The analysis of ankle muscle activation during gait better characterizes the response to ITB bolus injection than gait kinematics.
A Smart Trocar for Automatic Tool Recognition in Laparoscopic Surgery
Authors: Toti Giulia , Garbey Marc , Sherman Vadim , Bass Barbara L. , Dunkin Brian J.
Published: May 2014

Background. Operating rooms have become increasingly complex environments and more prone to errors because of loss of situation awareness. Adding computer intelligence to the operating room may help overcome these limitations particularly if the system can automatically track which step of an operation a surgeon is performing. To develop such a platform, it is necessary to track which laparoscopic instruments are being used and in which port they are inserted. This article describes the development and validation of a “Smart Trocar” that can automatically perform this function. Methods. A Smart Trocar system prototype was developed that uses a wireless camera attached to a standard laparoscopic port and custom software algorithms. The system recognizes color wheels attached to the handle of a laparoscopic instrument and compares the unique color pattern to an instrument library for proper tool identification. The system was tested for reliability in a box trainer environment using a variety of tool positions and levels of room light illumination. Results. Correct color classification was achieved in 96.7% of trials. There were no errors in detection of the color wheel in space. In addition, the distance of the color wheel from the camera did not influence results and correct classifications were evenly distributed among the 12 laparoscopic tool positions tested. Conclusion. This work describes a Smart Trocar system that identifies which laparoscopic tool is being used and in which port and proves its reliability. The system is an important element of a more comprehensive program being developed to automatically understand what step of an operation a surgeon is performing and use these data to improve situation awareness in the operating room.
Hygrothermal performance of internally insulated brick wall in cold climate: A case study in a historical school building
Authors: Klõšeiko Paul , Arumägi Endrik , Kalamees Targo
Published: May 2014

Interior thermal insulation is frequently one of the only possible solutions for thermal upgrade of the building envelope where the external appearance cannot be changed. In this study, four insulation materials were used in a case study in a historical school building in in situ test walls. The indoor climate in the test room was controlled to simulate the typical dwelling with high moisture load. The temperatures, relative humidity, and heat flows were monitored over 9 months to analyze the hygrothermal performance of four different insulation materials. The hygrothermal performance of insulation materials during drying and wetting periods are presented. Moisture test reference year was used in working out possible energy-renovation solutions. The results show that timing of the renovation works is a matter of consideration to avoid the hygrothermal risks inside the renovated wall assemblies. The results show that in all the cases, thermal comfort can be improved by increasing the inner surface temperature and decreasing thermal conductivity. However, in some cases, the risks of mold growth and interstitial condensation were present inside the retrofitted wall assemblies. Computer simulations of the wall assemblies with moisture reference years under different humidity loads concluded that all solutions are suitable for future analysis.
Successful pregnancy outcome in a woman with Turner’s syndrome
Authors: Church E , Bellis A , O’Connell I , Naqvi N
Published: May 2014

Women with Turner’s syndrome have a high incidence of cardiovascular complications, endocrine and hypertensive disorders. Those with the 45X chromosome complement require oocyte donation and in vitro fertilisation to conceive. Pregnancies in such women are challenging to manage due to the high risk of pregnancy-related hypertensive disorders, impaired glucose tolerance, fetal growth restriction and preterm birth. Women also need to be aware of the significant risk of aortic dilatation, dissection or rupture in pregnancy, which may be fatal. Despite these risks, favourable obstetric outcomes are achievable with careful pre-pregnancy counselling and cardiovascular assessment, intensive multidisciplinary antenatal monitoring and individualised delivery planning. We report the case of a 33-year-old woman with Turner’s syndrome, pre-existing hypertension, insulin-dependent diabetes and primary hypoparathyroidism who had a successful pregnancy with good maternal and fetal outcomes despite the complexity of her medical conditions.
Medical writing, editing and publishing
Authors: Simon Chantal
Published: May 2014

Although the core function of general practice is patient care, there are many opportunities for GPs to get involved in other educational and academic activities. Most GPs find academic activities a useful adjunct to their clinical work. As well as being educational and broadening horizons, such activities stimulate GPs to think and reappraise their own clinical work and the work of their practice. This article aims to give an overview of the range of opportunities available within medical publishing and some tips on how to access those opportunities.
Olecranon bursitis: a systematic overview
Authors: Blackwell John R , Hay Bruce A , Bolt Alexander M , Hay Stuart M
Published: May 2014

Background

Olecranon bursitis is a common condition where the bursal cavity, superficial to the olecranon, becomes inflamed. This can occur either with or without infection and has been given pseudonyms relating to the repeated minor trauma from external pressure that often predisposes. As a result of the multiple aetiologies, olecranon bursitis can present to any medical specialty with reasonable frequency and, although many therapies are described, a single, evidence-based and standardized treatment pathway is not well described.

Methods

We summarize the key points within the literature and subsequently propose an evidence-based treatment pathway.

Results

Relevant evidence is presented from appropriate publications to add rational to existing decision-making processes, together with personal experience and suggested operative bursectomy techniques from an established upper limb surgeon. The common and significant aetiologies are summarized and, in particular, red flag symptoms are highlighted by way of warning to the unsuspecting investigator.

Conclusions

The conclusion is provided in diagrammatic form, providing a suggested treatment pathway from history and examination through to operative intervention.
The Differential Effects of the Quality and Quantity of Online Reviews on Hotel Room Sales
Authors: Blal Inès , Sturman Michael C.
Published: May 2014

While it is generally accepted that hotel reviews and ratings posted on travel websites drive hotel sales and revenue, the effects of reviews can be parsed into volume (the number of reviews about a hotel) and valence (the ratings in those reviews). This study finds that the two chief aspects of reviews—volume and valence—have different effects on hotels in various chain scale segments. Industry reports and academic studies show that online reviews influence customers’ choice of hotel and thus drive hotels’ revenue per available room (RevPAR). However, the valence of those reviews has a greater effect on luxury hotels’ RevPAR, while the volume of reviews has a greater effect on lower-tier hotels. Based on a study of 319 hotels in the London metropolitan market, these effects apply equally to urban and suburban hotels, as well as chain and independent hotels. The results further indicate that the rating score effect on RevPAR has little impact on the economy and midscale segments, while an increasing number of reviews actually has negative effects on higher-end hotels.
Analysis of ferrite formed in 321 grade austenitic stainless steel
Authors: Green G. , Higginson R. , Hogg S. , Spindler S. , Hamm C. , Najorka J.
Published: May 2014

A significant fraction of ferrite has been identified in a 321 grade austenitic stainless steel in the solution heat treated condition. The microstructures were analysed using electron backscatter diffraction, energy dispersive X-ray spectroscopy and X-ray diffraction (XRD) and the stability of the ferrite investigated using heat treatments in a tube furnace, dilatometry and high temperature XRD. The ferrite dissolved ∼800°C, then formed again on cooling at temperatures under 200°C. Thermodynamic predictions showed a significant ferrite content at room temperature under equilibrium conditions, and the DeLong diagrams predict an austenite+martensite microstructure in the cast condition. Sensitivity analysis on the DeLong diagram has shown that the nitrogen content had a large effect on the austenite stability. The instability of the austenite and the subsequent transformation to ferrite on cooling can be attributed to low nitrogen content measured in the as received material. It was found that thermal aging of the material caused further transformation of austenite to ferrite as well as the formation of sigma phase that appears higher in nitrogen than the matrix phases. The diffusion of nitrogen into sigma phase may cause instability of the austenite, which could cause further transformation of austenite to ferrite on cooling from the aging temperature. The transformation of austenite to ferrite is known to be accompanied by an increase in volume, which may be of relevance to components made with tight dimensional tolerances.
Mapping hematopoiesis in a fully regenerative vertebrate: the axolotl
Authors: Lopez David , Lin Li , Monaghan James R. , Cogle Christopher R. , Bova Frank J. , Maden Malcolm , Scott Edward W.
Published: May 2014

Key Points

Establishing HSC transplantation and assay methods for the axolotl. Axolotl sites of hematopoiesis are the spleen and liver.
Measurement precision and biological variation of cranial arteries using automated analysis of 3 T magnetic resonance angiography
Authors: Amin Faisal Mohammad , Lundholm Elisabet , Hougaard Anders , Arngrim Nanna , Wiinberg Linda , de Koning Patrick JH , Larsson Henrik BW , Ashina Messoud
Published: May 2014

Background

Non-invasive magnetic resonance angiography (MRA) has facilitated repeated measurements of human cranial arteries in several headache and migraine studies. To ensure comparability across studies the same automated analysis software has been used, but the intra- and interobserver, day-to-day and side-to-side variations have not yet been published. We hypothesised that the observer related, side-to-side, and day-to-day variations would be less than 10%.

Methods

Ten female participants were studied using high-resolution MRA on two study days separated by at least one week. Using the automated LKEB-MRA vessel wall analysis software arterial circumferences were measured by blinded observers. Each artery was analysed twice by each of the two different observers. The primary endpoints were to determine the intraclass correlation coefficient (ICC) and intra- an inter-observer, the day-to-day, and side-to-side variations of the circumference of the middle meningeal (MMA) and middle cerebral (MCA) arteries.

Results

We found an excellent intra- and interobserver agreement for the MMA (ICC: 0.909-0.987) and for the MCA (ICC: 0.876-0.949). The coefficient of variance within observers was ≤1.8% for MMA and ≤3.1% for MCA; between observers ≤3.4% (MMA) and ≤4.1% (MCA); between days ≤6.0% (MMA) and ≤8.0% (MCA); between sides ≤9.4% (MMA) and ≤6.5% (MCA).

Conclusion

The present study demonstrates a low (<5%) inter- and intraobserver variation using the automated LKEB-MRA vessel wall analysis software. Furthermore, the study also suggests that the day-to-day and side-to-side variations of the MMA and MCA circumferences are less than 10%.
Polygonum viviparum L. induces vasorelaxation in the rat thoracic aorta via activation of nitric oxide synthase in endothelial cells
Authors: Chang Ming-Long , Chang Jung-Su , Yu Wen-Yu , Cheah Khoot-Peng , Li Joe-Sharg , Cheng Hui-Wen , Hu Chien-Ming
Published: May 2014

Background

In the past several decades, Polygonum viviparum L. (PV) was reported to have antibacterial, antiulcer, antioxidant, antitumor, anti-inflammatory, and antiarthritic properties. The anti-inflammatory pathway was recently elucidated through cytosolic nuclear factor E2-related factor 2 (Nrf2) activation and heme oxygenase (HO)-1 protein expression. PV is a perennial herb and widely distributed in high-elevation mountain regions, such as the Tibetan Plateau. In Tibetan traditional medicine, PV is usually used to boost the blood circulation to dissipate blood stasis. Therefore, this study focused on how PV improves the vascular circulation and acts on vascular tissues.

Methods

In this study, we isolated aortas from Sprague-Dawley rats (male, weight about 250 ~ 350 g), and detected the effects of PV on phenylephrine (PE)-induced contraction and cyclic guanosine 3′,5′-monophosphate (cGMP) formation using aortic rings. In addition, human umbilical vein endothelial cells (HUVECs) were used to exam nitric oxygen (NO) synthase (NOS) activity by directly measuring NO production in the culture medium. Endothelial (e) NOS phosphorylation, and cytosolic Nrf2 and HO-1 expressions were measured using a Western blot analysis.

Results

PV dose-dependently relaxed PE-induced contractions in endothelial-intact but not -denuded aorta. The concentration to produce 50% relaxation was 22.04 ± 1.77 μg/ml. PV-induced vasorelaxation was markedly blocked by pretreatment with N
G-nitro-L-arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue (MB), a guanylyl cyclase inhibitor, and hemoglobin, an NO scavenger. PV increased cGMP formation; however, this effect was also suppressed by co-pretreatment with l-NAME, MB, hemoglobin, and Ca2+-free medium. In HUVECs, PV increased NO formation, which was greatly attenuated by NOS inhibitors (L-NAME and L-NMMA) and by removing extracellular Ca2+ and chelating intracellular Ca2+ with BAPTA-AM. In addition, PV promoted eNOS phosphorylation, Nrf2 degradation, and HO-1 protein expression according to a Western blot analysis.

Conclusions

The results suggest that PV possesses vasorelaxing action in an endothelium-dependent manner and works through activating Ca2+/calmodulin- dependent NO synthesis; when NO is released and then transferred to smooth muscle cells, NO activates guanylyl cyclase and increases cGMP formation, ultimately resulting in vasorelaxation. Thus, PV can be considered for application as a potential therapeutic approach for vascular-associated disorders.
Quality of reporting on randomized controlled trials of acupuncture for stroke rehabilitation
Authors: Zhuang Lixing , He Jun , Zhuang Xun , Lu Liming
Published: May 2014

Background

Results from clinical studies on acupuncture for stroke rehabilitation are contradictory. The reason for the inconsistent findings especially lie in the transparency and accuracy of randomized controlled trials (RCTs) reports. This study aims to analyze the quality of reporting and its correlates in RCTs on acupuncture for stroke rehabilitation.

Methods

Quality of reporting for included papers was assessed against a subset of criteria adapted from the CONSORT 2010 statement and STRICTA. An overall quality score (OQS) and a combined key methodological index score (MIS) was calculated for each trial. Then, factors associated with OQS and MIS were identified.

Results

A total of 15 RCTs were included in full text. The median OQS based on the CONSORT statement and STRICTA was 8 and 12, respectively. The significant predictors for CONSORT OQS was funding source, for STRICTA was year of publication. With regard to the MIS, no variable was associated with improved methodological quality.

Conclusions

Our study found that the overall quality of reporting on RCTs of acupuncture for stroke rehabilitation was general or good. But some items’ reporting was found where information was insufficient or inadequate in most studies which needed substantial improvement.
Clinical implications of AGBL2 expression and its inhibitor latexin in breast cancer
Authors: Zhang Hao , Ren Yuan , Pang Deyan , Liu Caigang
Published: May 2014

Background

We investigated the expression status of AGBL2 and its inhibitor latexin in breast cancer stem cells and its clinical implications in order to lay a foundation for managing breast cancer.

Methods

CD44+/CD24- tumor cells (CSC) from clinical specimens were sorted using flow cytometry. AGBL2 expression status was detected in CSC and 126 breast cancer specimens by western blot and immunohistochemistry staining. The relationship between the AGBL2 protein and clinicopathological parameters and prognosis was subsequently determined.

Result

As a result, CSC are more likely to generate new tumors in mice and cell microspheres that are deficient in non-obese diabetic/severe combined immunodeficiency mice (NOD/SCID) compared to the control group. The AGBL2 protein was expressed higher in CSC induced to epithelial to mesenchymal transition (EMT) when compared to the control cells, and was found to be related to CSC chemotherapy resistance. After Spearman regression correlation analysis, AGBL2 was observed to be related to clinical stage, histological stage, and lymph node metastasis. In the Cox regression test, the AGBL2 protein was detected as an independent prognostic factor. Through immunoprecipitation, AGBL2 and latexin could form immune complexes.

Conclusions

These results demonstrate that AGBL2 is a latexin- interacting protein that regulates the tubulin tyrosination cycle and is a potential target for intervention.
Synthesis of silver nanoparticles and antibacterial property of silk fabrics treated by silver nanoparticles
Authors: Zhang Guangyu , Liu Yan , Gao Xiaoliang , Chen Yuyue
Published: May 2014

A silver nanoparticle solution was prepared in one step by mixing AgNO3 and a multi-amino compound (RSD-NH2) solution under ambient condition. RSD-NH2 was in-house synthesized by methacrylate and polyethylene polyamine in methanol, which has abundant amino and imino groups. However, the characterization of silver nanoparticles indicated that these nanoparticles are easy to agglomerate in solution. Therefore, an in situ synthesis method of silver nanoparticles on the silk fabrics was developed. The examined results confirmed that the in situ synthesized silver nanoparticles were evenly distributed on the surface of fibers. The inhibition zone test and the antibacterial rate demonstrated that the finished fabrics have an excellent antibacterial property against Staphylococcus aureus and Escherichia coli. Moreover, the nanosilver-treated silk fabrics were laundered 0, 5, 10, 20, and 50 times and still retained the exceptional antibacterial property. When the treated fabrics were washed 50 times, the antibacterial rate is more than 97.43% for S. aureus and 99.86% for E. coli. The excellent laundering durability may be attributed to the tight binding between silver nanoparticles and silk fibers through the in situ synthesis. This method provides an economic method to enhance the antibacterial capability of silk fabrics with good resistance to washings.
A biocompatible synthesis of gold nanoparticles by Tris(hydroxymethyl)aminomethane
Authors: Chen Feng , Wang Yanwei , Ma Jun , Yang Guangcan
Published: May 2014

Gold nanoparticles' novel properties are widely realized in catalysis, plasmonics, electronics, and biomedical applications. For biomedical application, one challenge is to find a non-toxic chemical and/or physical method of functionalizing gold nanoparticles with biomolecular compounds that can promote efficient binding, clearance, and biocompatibility and to assess their safety to other biological systems and their long-term effects on human health and reproduction. In the present study, we describe a new method by using Tris(hydroxymethyl)aminomethane (Tris), a widely used buffer solvent of nucleic acid and proteins, as the reducing agent for synthesizing gold nanoparticles by one step. It is found that Tris carries out the reduction reactions in relatively mild conditions for biomacromolecules. Particularly, it can be used to modify the DNA during the process of preparation of gold nanoparticles. The morphology and size distribution of gold nanoparticles are consistent and were confirmed by many different approaches including dynamic light scattering (DLS), UV-visible (UV-vis) spectrophotometry, atomic force microscopy (AFM), and transmission electron microscopy (TEM).
Fabrication of ZnCoO nanowires and characterization of their magnetic properties
Authors: Kim Bum-Su , Lee Seunghun , Kim Won-Kyung , Park Ji-Hun , Cho Yong Chan , Kim Jungdae , Cho Chae Ryong , Jeong Se-Young
Published: May 2014

Hydrogen-treated ZnCoO shows magnetic behavior, which is related to the formation of Co-H-Co complexes. However, it is not well known how the complexes are connected to each other and with what directional behavior they are ordered. In this point of view, ZnCoO nanowire is an ideal system for the study of the magnetic anisotropy. ZnCoO nanowire was fabricated by trioctylamine solution method under different ambient gases. We found that the oxidation of trioctylamine plays an essential role on the synthesis of high-quality ZnCoO nanowires. The hydrogen injection to ZnCoO nanowires induced ferromagnetism with larger magnetization than ZnCoO powders, while becoming paramagnetic after vacuum heat treatment. Strong ferromagnetism of nanowires can be explained by the percolation of Co-H-Co complexes along the c-axis.
Moisture barrier properties of thin organic-inorganic multilayers prepared by plasma-enhanced ALD and CVD in one reactor
Authors: Bülow Tim , Gargouri Hassan , Siebert Mirko , Rudolph Rolf , Johannes Hans-Hermann , Kowalsky Wolfgang
Published: May 2014

A widely used application of the atomic layer deposition (ALD) and chemical vapour deposition (CVD) methods is the preparation of permeation barrier layers against water vapour. Especially in the field of organic electronics, these films are highly demanded as such devices are very sensitive to moisture and oxygen. In this work, multilayers of aluminium oxide (AlO
x
) and plasma polymer (PP) were coated on polyethylene naphthalate substrates by plasma-enhanced ALD and plasma-enhanced CVD at 80â„ƒ in the same reactor, respectively. As precursor, trimethylaluminium was used together with oxygen radicals in order to prepare AlO
x
, and benzene served as precursor to deposit the PP. This hybrid structure allows the decoupling of defects between the single AlO
x
layers and extends the permeation path for water molecules towards the entire barrier film. Furthermore, the combination of two plasma techniques in a single reactor system enables short process times without vacuum breaks. Single aluminium oxide films by plasma-enhanced ALD were compared to thermally grown layers and showed a significantly better barrier performance. The water vapour transmission rate (WVTR) was determined by means of electrical calcium tests. For a multilayer with 3.5 dyads of 25-nm AlO
x
and 125-nm PP, a WVTR of 1.2 × 10 −3 gm
−2
d
−1 at 60â„ƒ and 90% relative humidity could be observed.
Robust exponential stability analysis for delayed neural networks with time-varying delay
Authors: Xie Jing-Chen , Chen Chin-Pin , Liu Pin-Lin , Jeng Yoau-Chau
Published: May 2014

This paper considers the problems of determining the robust exponential stability and estimating the exponential convergence rate for delayed neural networks with parametric uncertainties and time delay. The relationship among the time-varying delay, its upper bound, and their difference is taken into account. Theoretic analysis shows that our result includes a previous result derived in the literature. As illustrations, the results are applied to several concrete models studied in the literature, and a comparison of results is given.
Cinnarizine for the prophylaxis of migraine associated vertigo: a retrospective study
Authors: Taghdiri Foad , Togha Mansoureh , Razeghi Jahromi Soodeh , Refaeian Farshid
Published: May 2014

Objective

To assess the efficacy and safety of cinnarizine for the prophylaxis of migraine associated vertigo in the vestibular migraine and migraine with brainstem aura.

Background

Vestibular migraine and migraine with brainstem aura are two principal clinical syndromes that frequently are associated with vertigo. Since cinnarizine is a well-tolerated calcium channel blocker which has acceptable effect on both vertigo and migraine headache, we carried out this study to evaluate the efficacy and safety of this medication in vestibular migraine and also migraine with brainstem aura associated with vertigo.

Methods

This was a retrospective, single-center, open-label, investigation of the effects of cinnarizine on vestibular migraine and migraine with associated with vertigo. We assessed the change in monthly frequency of vertigo and also frequency, duration and intensity of migraine attacks after one, two and three months of cinnarizine administration.

Results

The mean frequency of vertigo and also the mean frequency, duration and intensity of migraine headaches per month were reduced significantly after three months of cinnarizine therapy (all p < 0.001).

Conclusion

This study suggests that cinnarizine is safe and effective in reducing both headache and vertigo aspects of “migraine plus vertigo” among the patients who suffer from either vestibular migraine or migraine with brainstem aura associated with vertigo.
Levels of cerebrospinal fluid α-synuclein oligomers are increased in Parkinson’s disease with dementia and dementia with Lewy bodies compared to Alzheimer’s disease
Authors: Hansson Oskar , Hall Sara , Öhrfelt Annika , Zetterberg Henrik , Blennow Kaj , Minthon Lennart , Nägga Katarina , Londos Elisabet , Varghese Shiji , Majbour Nour K , Al-Hayani Abdulmonem , El-Agnaf Omar MA
Published: May 2014

Introduction

The objective was to study whether α-synuclein oligomers are altered in the cerebrospinal fluid (CSF) of patients with dementia, including Parkinson disease with dementia (PDD), dementia with Lewy bodies (DLB), and Alzheimer disease (AD), compared with age-matched controls.

Methods

In total, 247 CSF samples were assessed in this study, including 71 patients with DLB, 30 patients with PDD, 48 patients with AD, and 98 healthy age-matched controls. Both total and oligomeric α-synuclein levels were evaluated by using well-established immunoassays.

Results

The levels of α-synuclein oligomers in the CSF were increased in patients with PDD compared with the controls (P < 0.05), but not in patients with DLB compared with controls. Interestingly, the levels of α-synuclein oligomers in the CSF were also significantly higher in patients with PDD (P < 0.01) and DLB (P < 0.05) compared with patients with AD. The levels of CSF α-synuclein oligomers and the ratio of oligomeric/total-α-synuclein could distinguish DLB or PDD patients from AD patients, with areas under the curves (AUCs) of 0.64 and 0.75, respectively. In addition, total-α-synuclein alone could distinguish DLB or PDD patients from AD patients, with an AUC of 0.80.

Conclusions

The levels of α-synuclein oligomers were increased in the CSF from α-synucleinopathy patients with dementia compared with AD cases.
Erythrocyte selenium concentration predicts intensive care unit and hospital mortality in patients with septic shock: a prospective observational study
Authors: Costa Nara Aline , Gut Ana Lúcia , Pimentel José Alexandre Coelho , Cozzolino Silvia Maria Franciscato , Azevedo Paula Schmidt , Fernandes Ana Angélica Henrique , Polegato Bertha Furlan , Tanni Suzana Erico , Gaiolla Rafael Dezen , Zornoff Leonardo Antonio Mamede , de Paiva Sergio Alberto Rupp , Minicucci Marcos Ferreira
Published: May 2014

Introduction

Selenoenzymes can modulate the extent of oxidative stress, which is recognized as a key feature of septic shock. The pathophysiologic role of erythrocyte selenium concentration in patients with septic shock remains unknown. Therefore, the objective of this study was to evaluate the association of erythrocyte selenium concentration with glutathione peroxidase (GPx1) activity, GPx1 polymorphisms and with ICU and hospital mortality in septic shock patients.

Methods

This prospective study included all patients older than 18 years with septic shock on admission or during their ICU stay, admitted to one of the three ICUs of our institution, from January to August 2012. At the time of the patients’ enrollment, demographic information was recorded. Blood samples were taken within the first 72 hours of the patients’ admission or within 72 hours of the septic shock diagnosis for determination of selenium status, protein carbonyl concentration, GPx1 activity and GPx1 Pro198Leu polymorphism (rs 1050450) genotyping.

Results

A total of 110 consecutive patients were evaluated. The mean age was 57.6 ± 15.9 years, 63.6% were male. Regarding selenium status, only erythrocyte selenium concentration was lower in patients who died in the ICU. The frequencies for GPx1 Pro198Leu polymorphism were 55%, 38% and 7% for Pro/Pro, Pro/Leu and Leu/Leu, respectively. In the logistic regression models, erythrocyte selenium concentration was associated with ICU and hospital mortality in patients with septic shock even after adjustment for protein carbonyl concentration and acute physiology and chronic health evaluation II score (APACHE II) or sequential organ failure assessment (SOFA).

Conclusions

Erythrocyte selenium concentration was a predictor of ICU and hospital mortality in patients with septic shock. However, this effect was not due to GPx1 activity or Pro198Leu polymorphism.
Trace determination of cadmium in water using anodic stripping voltammetry at a carbon paste electrode modified with coconut shell powder
Authors: Rajawat Deepak Singh , Kumar Nitin , Satsangee Soami Piara
Published: May 2014

Background

Increasing awareness on the environmental impact of heavy metals has increased a considerable interest in the determination of metals in natural water bodies. The present paper describes the development and electrochemical application of carbon paste electrode modified with fibrous part of coconut shell for the determination of cadmium in water samples.

Methods

Determination was carried out using anodic stripping voltammetry. It is a two-step process. First, the metal ions get accumulated at the electrode surface at open-circuit potential, followed by a potential scan for voltammetric determination of cadmium.

Results

Different parameters affecting the determination of Cd (II) were optimized and are as follows: HCl as stripping solvent, acetate buffer of pH 5 as accumulating solvent, and 15-min accumulation time. Triton X-100, cetyltrimethylammonium bromide, and sodium dodecyl sulfate were used as representative for neutral, cationic, and anionic surfactants, respectively, to see the effect of surface active macromolecules. Interference caused by other metal ions on the determination of cadmium was also studied.

Conclusions

The method shows the development of a sensor for the sensitive determination of cadmium with limit of detection at 105 μg L−1. This technique does not use mercury and, therefore, has a positive environmental benefit.
L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration
Authors: Serrano-Pertierra Esther , Cernuda-Morollón Eva , Brdička Tomáš , Hoøejši Václav , López-Larrea Carlos
Published: May 2014

Membrane rafts are microdomains of the plasma membrane that have multiple biological functions. The involvement of these structures in the biology of T cells, namely in signal transduction by the TCR, has been widely studied. However, the role of membrane rafts in immunoreceptor signaling in NK cells is less well known. We studied the distribution of the activating NKG2D receptor in lipid rafts by isolating DRMs in a sucrose density gradient or by raft fractionation by β-OG-selective solubility in the NKL cell line. We found that the NKG2D-DAP10 complex and pVav are recruited into rafts upon receptor stimulation. Qualitative proteomic analysis of these fractions showed that the actin cytoskeleton is involved in this process. In particular, we found that the actin-bundling protein L-plastin plays an important role in the clustering of NKG2D into lipid rafts. Moreover, coengagement of the inhibitory receptor NKG2A partially disrupted NKG2D recruitment into rafts. Furthermore, we demonstrated that L-plastin participates in NKG2D-mediated inhibition of NK cell chemotaxis.
WT1 regulates the development of the posterior taste field
Authors: Gao Yankun , Toska Eneda , Denmon Dane , Roberts Stefan G. E. , Medler Kathryn F.
Published: May 2014

Despite the importance of taste in determining nutrient intake, our understanding of the processes that control the development of the peripheral taste system is lacking. Several early regulators of taste development have been identified, including sonic hedgehog, bone morphogenetic protein 4 and multiple members of the Wnt/β-catenin signaling pathway. However, the regulation of these factors, including their induction, remains poorly understood. Here, we identify a crucial role for the Wilms' tumor 1 protein (WT1) in circumvallate (CV) papillae development. WT1 is a transcription factor that is important in the normal development of multiple tissues, including both the olfactory and visual systems. In mice, WT1 expression is detectable by E12.5, when the CV taste placode begins to form. In mice lacking WT1, the CV fails to develop normally and markers of early taste development are dysregulated compared with wild type. We demonstrate that expression of the WT1 target genes Lef1, Ptch1 and Bmp4 is significantly reduced in developing tongue tissue derived from Wt1 knockout mice and that, in normal tongue, WT1 is bound to the promoter regions of these genes. Moreover, siRNA knockdown of WT1 in cultured taste cells leads to a reduction in the expression of Lef1 and Ptch1. Our data identify WT1 as a crucial transcription factor in the development of the CV through the regulation of multiple signaling pathways that have established roles in the formation and patterning of taste placodes.
Variation in body condition during the post-moult foraging trip of southern elephant seals and its consequences on diving behaviour
Authors: Richard Gaëtan , Vacquié-Garcia Jade , Jouma'a Joffrey , Picard Baptiste , Génin Alexandre , Arnould John P. Y. , Bailleul Frédéric , Guinet Christophe
Published: May 2014

Mature female southern elephant seals come ashore only in October to breed and in January to moult, spending the rest of the year foraging at sea. Mature females may lose as much as 50% of their body mass, mostly in lipid stores, during the breeding season due to fasting and lactation. When departing to sea, post-breeding females are negatively buoyant and the relative change in body condition (i.e. density) during the foraging trip has previously been assessed by monitoring descent rate during drift dives. However, relatively few drift dives are performed resulting in low resolution of the temporal reconstruction of body condition change. In this study, six post-breeding females were instrumented with time-depth recorders and accelerometers to investigate whether changes in active swimming effort and speed could be used as an alternative method of monitoring density variations throughout the foraging trip. In addition, we assessed consequences of density change on the swimming effort of individuals while diving and effects on dive duration. Both descent swimming speed and ascent swimming effort were found to be strongly correlated to descent rate during drift dives, enabling the fine-scale monitoring of seal density change over the whole trip. Negatively buoyant seals minimized swimming effort during descents, gliding down at slower speeds, and reduced their ascent swimming effort to maintain a nearly constant swimming speed as their buoyancy increased. One percent of seal density variation over time was found to induce a 20% variation in swimming effort during dives with direct consequences on dive duration.
Living in warmer more acidic oceans retards physiological recovery from tidal emersion in the velvet swimming crabNecora puber(L.)
Authors: Rastrick Samuel P.S. , Calosi Piero , Calder-Potts Ruth , Foggo Andy , Nightingale Gregory , Widdicombe Stephen , Spicer John I.
Published: May 2014

The distribution patterns of many species in the intertidal zone are partly determined by their ability to survive and recover from tidal emersion. During emersion most crustaceans experience gill-collapse impairing gas-exchange. Such collapse generates a state of hypoxemia and a hypercapnia-induced respiratory acidosis, leading to hyperlactaemia and metabolic acidosis. However, how such physiological responses to emersion are modified by prior exposure to elevated CO2 and temperature combinations, indicative of future climate change scenarios, is not known. We therefore investigated key physiological responses of velvet swimming crabs, Necora puber, kept for 14 days at one of four pCO2/temperature treatments (400 μatm/10 °C, 1000 μatm/10 °C, 400 μatm/15 °C, 1000 μatm/15 °C), to experimental emersion and recovery. Pre-exposure to elevated pCO2 and temperature increased pre-emersion bicarbonate ion concentrations [HCO3-], increasing resistance to short periods of emersion (90 min). However, there was still a significant acidosis following 180 min emersion in all treatments. The recovery of extracellular acid base via the removal of extracellular pCO2 (PCO2) and lactate after emersion was significantly retarded by exposure to both elevated temperature and pCO2. If elevated environmental pCO2 and temperature lead to slower recovery after emersion, then some predominantly subtidal species that also inhabit the low to mid shore, such as N. puber, may have a reduced physiological capacity to retain their presence in the low intertidal zone, ultimately affecting their bathymetric range of distribution, as well as the structure, and diversity of intertidal assemblages.
Ant-fungal species combinations engineer physiological activity of fungus gardens
Authors: Seal Jon N. , Schiøtt Morten , Mueller Ulrich G.
Published: May 2014

Fungus-gardening insects are among the most complex organisms due to their extensive coevolutionary histories with obligate fungal symbionts and other microbes.Some fungus-gardening insect lineages share fungal symbionts with other members of their lineage and thus exhibit diffuse co-evolutionary relationships, while others exhibit little or no symbiont sharing, resulting in host-fungus fidelity. The mechanisms that maintain this symbiont fidelity are currently unknown. Prior work suggested that derived leaf-cutting ants in the genus Atta interact synergistically with leaf-cutter fungi ('Attamyces') by exhibiting higher fungal growth rates and enzymatic activities than when growing a fungus from the sister-clade to Attamyces (so-called 'Trachymyces') grown primarily by the non-leaf cutting Trachymyrmex ants that form, correspondingly, the sister-clade to leafcutting ants. To elucidate the enzymatic bases of host-fungus specialization in leafcutting ants, we conducted a reciprocal fungus-switch experiment between the ant Atta texana and the ant Trachymyrmex arizonensis and report measured enzymatic activities of switched, and sham-switched fungus gardens to digest starch, pectin, xylan, cellulose, and casein. Gardens exhibited higher amylase and pectinase activities when A. texana ants cultivated Attamyces compared to Trachymyces fungi, consistent with enzymatic specialization. In contrast, gardens showed comparable amylase and pectinase activities when T. arizonensis cultivated either fungal species. Although gardens of leaf-cutting ants are not known to be significant metabolizers of cellulose, T. arizonensis were able to maintain gardens with significant cellulase activity when growing either fungal species. In contrast to carbohydrate metabolism, protease activity was significantly higher in Attamyces than in Trachymyces, regardless of the ant host. Activity of some enzymes employed by this symbiosis therefore arises from complex interactions between ant-host and fungal-symbiont.
Gait-specific adaptation of locomotor activity in response to dietary restriction inCaenorhabditis elegans
Authors: Lüersen Kai , Faust Ulla , Gottschling Dieter-Christian , Döring Frank
Published: May 2014

Locomotion is crucial for the survival of living organisms, as it allows foraging, flight and mating behaviour. In response to environmental cues, many organisms switch between alternative forms of locomotion, referred to as gaits. The nematode Caenorhabditis elegans exhibits two gaits: swimming in liquids and crawling on dense gels. The kinematics and patterns of muscle activity differ between the two gaits, with swimming being less efficient than crawling. We found that C. elegans when grown on dietary restriction (DR) plates and then tested immediately for swimming activity exhibit an accelerated frequency of body-bending swimming compared to ad libitum fed worms, resulting in an increased swimming speed. This response is independent of the presence or absence of food bacteria in the assay liquid. In contrast, the crawling speed of DR worms on assay agar plates is decreased and influenced by food availability. Since DR also attenuates the disturbed swimming activity of worms that are deficient in the presynaptic dopamine transporter DAT-1, our data link DR induced alterations of the swimming gait to synaptic processes. This strongly suggests a biochemical rather than a biomechanical response to DR provoked by changes in the worm's body structure. We conclude that the increase in locomotor activity in response to DR is specific to the swimming gait and might represent a survival strategy, allowing food-deprived nematodes to exit unfavourable environments.
Following the Sun: a mathematical analysis of the tracks of clock-shifted homing pigeons
Authors: Schiffner Ingo , Siegmund Bettina , Wiltschko Roswitha
Published: May 2014

We analyse the tracks of clock-shifted pigeons from six releases to determine how pigeons cope with the conflict between their sun compass and the other navigational cues. Time-lag embedding is used to calculate the short-term correlation dimension, a parameter that reflects the complexity of the navigational system and with it, the number of factors involved. In the beginning, while pigeons are still at the release site, the short-term correlation dimension is low; it increases as the birds leave the site, indicating the birds are now actively navigating. Clock-shifted pigeons show more scatter than the control birds, and their short-term correlation dimension becomes significantly smaller than that of the controls, remaining lower until the experimental birds reach their loft. This difference is small, but consistent and suggests a different rating and ranking of the navigational cues. Clock-shifted pigeons do not seem to simply ignore the information from their manipulated sun compass altogether, but appear to merely downgrade it in favour of other cues, like their magnetic compass. This is supported by observation that the final part of the tracks still shows a small deviation into the expected direction, indicating an effect of clock-shifting until the end of the homing flight.
Mitochondrial phenotypic flexibility enhances energy savings during winter fast in king penguin chicks
Authors: Monternier Pierre-Axel , Marmillot Vincent , Rouanet Jean-Louis , Roussel Damien
Published: May 2014

Energy conservation is a key priority for organisms living in environments with seasonal shortages in resource supplies or spontaneously fasting during their annual cycle. The aim of the study was to determine whether the high fasting endurance of winter-acclimatized king penguin chicks (Aptenodytes patagonicus) would be associated with an adjustment of mitochondrial bioenergetics in pectoralis muscle, the largest skeletal muscle in penguins. The rates of mitochondrial oxygen consumption and ATP synthesis and mitochondrial efficiency (ATP/O ratio) were measured in winter-acclimatized chicks. We used pyruvate/malate and palmitoyl-L-carnitine/malate as respiratory substrates and results from naturally fasted chicks were compared to experimentally re-fed chicks. Bioenergetics analysis of pectoralis muscle revealed that mitochondria are on average 15% more energy efficient in naturally fasted than in experimentally fed chicks, indicating that fasted birds would consume fewer nutrients to sustain their energy demanding processes. We also found that moderate reductions in temperature from 38°C to 30°C further increase by 23% the energy coupling efficiency at the level of mitochondria, suggesting that king penguin chicks realize additional energy savings while becoming hypothermic during winter. It has been calculated that this adjustment of mitochondrial efficiency in skeletal muscle may contribute to nearly 25% of fasting-induced reduction in mass-specific metabolic rate measured in vivo. The present study shows that the regulation of mitochondrial efficiency triggers the development of an economical management of resources, which would maximize the conservation of endogenous fuel store by decreasing the cost of living in fasted winter-acclimatized king penguin chicks.