bluuebunny/crossref_metadata_2025 huggingface dataset:
The Noisy and Marvelous Molecular World of Biology
Authors: Ritort Felix
Published: April 2019

At the molecular level biology is intrinsically noisy. The forces that regulate the myriad of molecular reactions in the cell are tiny, on the order of piconewtons (10−12 Newtons), yet they proceed in concerted action making life possible. Understanding how this is possible is one of the most fundamental questions biophysicists would like to understand. Single molecule experiments offer an opportunity to delve into the fundamental laws that make biological complexity surface in a physical world governed by the second law of thermodynamics. Techniques such as force spectroscopy, fluorescence, microfluidics, molecular sequencing, and computational studies project a view of the biomolecular world ruled by the conspiracy between the disorganizing forces due to thermal motion and the cosmic evolutionary drive. Here we will digress on some of the evidences in support of this view and the role of physical information in biology.
Non-Invasive Imaging in Diabetic Cardiomyopathy
Authors: Mordi Ify R.
Published: April 2019

There is increasing recognition of a specific diabetic cardiomyopathy beyond ischemic cardiomyopathy, which leads to structural and functional myocardial abnormalities. The aim of this review is to summarize the recent literature on diagnostic findings and prognostic significance of non-invasive imaging including echocardiography, nuclear imaging, computed tomography and cardiovascular magnetic resonance in diabetic cardiomyopathy.
Bicuspid Aortic Valve Alters Aortic Protein Expression Profile in Neonatal Coarctation Patients
Authors: Skeffington Katie L. , Bond Andrew R. , Abdul-Ghani Safa , Iacobazzi Dominga , Kang Sok-Leng , Heesom Kate J. , Wilson Marieangela C. , Ghorbel Mohamed , Stoica Serban , Martin Robin , Suleiman M. Saadeh , Caputo Massimo
Published: April 2019

Coarctation of the aorta is a form of left ventricular outflow tract obstruction in paediatric patients that can be presented with either bicuspid (BAV) or normal tricuspid (TAV) aortic valve. The congenital BAV is associated with hemodynamic changes and can therefore trigger different molecular remodelling in the coarctation area. This study investigated the proteomic and phosphoproteomic changes associated with BAV for the first time in neonatal coarctation patients. Aortic tissue was collected just proximal to the coarctation site from 23 neonates (BAV; n = 10, TAV; n = 13) that were matched for age (age range 4–22 days). Tissue from half of the patients was frozen and used for proteomic and phosphoproteomic analysis whilst the remaining tissue was formalin fixed and used for analysis of elastin content using Elastic Van-Gieson (EVG) staining. A total of 1796 protein and 75 phosphoprotein accession numbers were detected, of which 34 proteins and one phosphoprotein (SSH3) were differentially expressed in BAV patients compared to TAV patients. Ingenuity Pathway Analysis identified the formation of elastin fibres as a significantly enriched function (p = 1.12 × 10−4) due to the upregulation of EMILIN-1 and the downregulation of TNXB. Analysis of paraffin sections stained with EVG demonstrated increased elastin content in BAV patients. The proteomic/phosphoproteomic analysis also suggested changes in inositol signalling pathways and reduced expression of the antioxidant SOD3. This work demonstrates for the first time that coarcted aortic tissue in neonatal BAV patients has an altered proteome/phosphoproteome consistent with observed structural vascular changes when compared to TAV patients.
Evaluating Human Photoreceptoral Inputs from Night-Time Lights Using RGB Imaging Photometry
Authors: Sánchez de Miguel Alejandro , Bará Salvador , Aubé Martin , Cardiel Nicolás , Tapia Carlos E. , Zamorano Jaime , Gaston Kevin J.
Published: April 2019

Night-time lights interact with human physiology through different pathways starting at the retinal layers of the eye; from the signals provided by the rods; the S-, L- and M-cones; and the intrinsically photosensitive retinal ganglion cells (ipRGC). These individual photic channels combine in complex ways to modulate important physiological processes, among them the daily entrainment of the neural master oscillator that regulates circadian rhythms. Evaluating the relative excitation of each type of photoreceptor generally requires full knowledge of the spectral power distribution of the incoming light, information that is not easily available in many practical applications. One such instance is wide area sensing of public outdoor lighting; present-day radiometers onboard Earth-orbiting platforms with sufficient nighttime sensitivity are generally panchromatic and lack the required spectral discrimination capacity. In this paper, we show that RGB imagery acquired with off-the-shelf digital single-lens reflex cameras (DSLR) can be a useful tool to evaluate, with reasonable accuracy and high angular resolution, the photoreceptoral inputs associated with a wide range of lamp technologies. The method is based on linear regressions of these inputs against optimum combinations of the associated R, G, and B signals, built for a large set of artificial light sources by means of synthetic photometry. Given the widespread use of RGB imaging devices, this approach is expected to facilitate the monitoring of the physiological effects of light pollution, from ground and space alike, using standard imaging technology.
What They Did Not Tell You about Algebraic (Non-) Existence, Mathematical (IR-)Regularity and (Non-) Asymptotic Properties of the Full BEKK Dynamic Conditional Covariance Model
Authors: McAleer Michael
Published: April 2019

Persistently high negative covariances between risky assets and hedging instruments are intended to mitigate against risk and subsequent financial losses. In the event of having more than one hedging instrument, multivariate covariances need to be calculated. Optimal hedge ratios are unlikely to remain constant using high frequency data, so it is essential to specify dynamic covariance models. These values can either be determined analytically or numerically on the basis of highly advanced computer simulations. Analytical developments are occasionally promulgated for multivariate conditional volatility models. The primary purpose of the paper is to analyze purported analytical developments for the most widely-used multivariate dynamic conditional covariance model to have been developed to date, namely the Full BEKK model, named for Baba, Engle, Kraft and Kroner. Dynamic models are not straightforward (or even possible) to translate in terms of the algebraic existence, underlying stochastic processes, specification, mathematical regularity conditions, and asymptotic properties of consistency and asymptotic normality, or the lack thereof. The paper presents a critical analysis, discussion, evaluation and presentation of caveats relating to the Full BEKK model, and an emphasis on the numerous dos and don’ts in implementing the Full BEKK and related non-Diagonal BEKK models, such as Triangular BEKK and Hadamard BEKK, in practice.
Amorphous Carbon-Induced Surface Defect Repair for Reinforcing the Mechanical Properties of Carbon Fiber
Authors: Li Dachao , Liu Hongzhong , Chen Bangdao , Niu Dong , Lei Biao , Ye Guoyong , Jiang Weitao , Shi Yongsheng , Yin Lei , Lai Guoquan
Published: April 2019

Graphene oxide (GO) was prepared using metal-catalyzed crystallization of amorphous carbon on a carbon fiber surface to improve the mechanical properties of the carbon fiber (CF). The deposited GO was used for repairing of surface structure defects on CF, thereby improving the tensile strength and interfacial strength force of CF. The grown morphology of GO and the changes in CF surface microstructure before and after remediation were investigated in detail by scanning tunneling microscopy and Raman spectroscopy. The effects of surface repair on the mechanical properties of the CF and the resulting composites were investigated systematically. The results of scanning tunneling microscopy show that the graphene oxide formed on the surface of carbon fiber present uniform dispersion. Raman spectroscopy curves indicate that CF successfully remediated the defects in the CF surface. The results of mechanical properties testing show that such a remediation method could significantly enhance the tensile strength of CF and increase the interfacial strength versus raw fibers; that is, the tensile strength of CF was enhanced by 42% and the interfacial strength by 33.7%.
Improved Mechanical and Moisture-Resistant Properties of Woven Hybrid Epoxy Composites by Graphene Nanoplatelets (GNP)
Authors: Naveen Jesuarockiam , Jawaid Mohammad , Zainudin Edi Syams , Thariq Hameed Sultan Mohamed , Yahaya Ridwan
Published: April 2019

This research investigated the effect of adding different wt.% (0, 0.25, 0.50, and 0.75) of GNP (graphene nanoplatelets) to improve the mechanical and moisture resistant properties of Kevlar (K)/cocos nucifera sheath (CS)/epoxy hybrid composites. The laminates were fabricated with different K/CS weight ratios such as 100/0 (S1), 75/25 (S2), 50/50 (S3), 25/75 (S4), and 0/100 (S5). The results revealed that the addition of GNP improved the tensile, flexural, and impact properties of laminated composites. However, the optimal wt.% of GNP varies with different laminates. A moisture diffusion analysis showed that the laminates with a 0.25 wt.% of GNP content efficiently hindered water uptake by closing all the unoccupied pores inside the laminate. Morphological investigations (SEM and FE-SEM (Field Emission Scanning Electron Microscope)) proved that the addition of GNP improved the interfacial adhesion and dispersion. Structural (XRD and FTIR) analyses reveals that at 0.25 wt.% of GNP, all the hybrid composites showed a better crystallinity index and the functional groups presents in the GNP can form strong interactions with the fibers and matrix. A statistical analysis was performed using One-way ANOVA, and it corroborates that the mechanical properties of different laminates showed a statistically significant difference. Hence, these GNP-modified epoxy hybrid composites can be efficiently utilized in load-bearing structures.
Analysis of the Influence of Blanking Clearance on the Wear of the Punch, the Change of the Burr Size and the Geometry of the Hook Blanked in the Hardened Steel Sheet
Authors: Mucha Jacek , Tutak Jacek
Published: April 2019

In this thesis, the results of an experimental analysis of blanking angled hooks with a punch of a sloping face in a thin steel sheet with a hardness of 55 HRC are presented. The blanking punch was made of K340 cold-work tool steel. Tests were carried out for three values of clearance, 5%, 10%, and 15% of sheet thickness. The results of the analysis of the influence of the number of cuts made by the punch on the growth of the burr at the sheet edge were presented. Moreover, the influences of the clearance on the initial values of burr (bh), deflection (Hb), and the bending radius (Rb) of the hook have been shown. The influence of the friction path on the intensity of degradation of working surfaces and the blanking edges of the punch was also demonstrated. The obtained results allow the selection of the proper clearance and new tool materials for blanking blades working in particularly difficult tribological conditions.
Research on the Buckling Behavior of Functionally Graded Plates with Stiffeners Based on the Third-Order Shear Deformation Theory
Authors: Nguyen Hoang-Nam , Tan Tran Cong , Luat Doan Trac , Phan Van-Duc , Thom Do Van , Minh Phung Van
Published: April 2019

This paper presents a finite element formulation to study the mechanical buckling of stiffened functionally graded material (FGM) plates. The approach is based on a third-order shear deformation theory (TSDT) introduced by Guangyu Shi. The material properties of the plate were assumed to be varied in the thickness direction by a power law distribution, but the material of the stiffener was the same as that of the one of the bottom surface where the stiffener was placed. A parametric study was carried out to highlight the effect of material distribution, the thickness-to-width ratio, and stiffener parameters on the buckling characteristics of the stiffened FGM plates. Numerical results showed that the addition of stiffener to the FGM plate could significantly reduce the weight of the FGM plate but that both the FGM plates with and without stiffener had equally high strength in the same boundary condition and compression loading.
A Novel Heterogeneous Swarm Reinforcement Learning Method for Sequential Decision Making Problems
Authors: Akbari Zohreh , Unland Rainer
Published: April 2019

Sequential Decision Making Problems (SDMPs) that can be modeled as Markov Decision Processes can be solved using methods that combine Dynamic Programming (DP) and Reinforcement Learning (RL). Depending on the problem scenarios and the available Decision Makers (DMs), such RL algorithms may be designed for single-agent systems or multi-agent systems that either consist of agents with individual goals and decision making capabilities, which are influenced by other agent’s decisions, or behave as a swarm of agents that collaboratively learn a single objective. Many studies have been conducted in this area; however, when concentrating on available swarm RL algorithms, one obtains a clear view of the areas that still require attention. Most of the studies in this area focus on homogeneous swarms and so far, systems introduced as Heterogeneous Swarms (HetSs) merely include very few, i.e., two or three sub-swarms of homogeneous agents, which either, according to their capabilities, deal with a specific sub-problem of the general problem or exhibit different behaviors in order to reduce the risk of bias. This study introduces a novel approach that allows agents, which are originally designed to solve different problems and hence have higher degrees of heterogeneity, to behave as a swarm when addressing identical sub-problems. In fact, the affinity between two agents, which measures the compatibility of agents to work together towards solving a specific sub-problem, is used in designing a Heterogeneous Swarm RL (HetSRL) algorithm that allows HetSs to solve the intended SDMPs.
The Quality of Life of Patients with Surgically Treated Mandibular Fractures and the Relationship of the Posttraumatic Pain and Trismus with the Postoperative Complications: A Prospective Study
Authors: Boljevic Tanja , Vukcevic Batric , Pesic Zoran , Boljevic Aleksandar
Published: April 2019

Background and objectives: Due to the fact that the mandible is the only movable bone in the face, it is often exposed to the influence of external forces. The incidence of trismus and posttraumatic pain in unilateral mandibular corpus fractures may be related to the occurrence of complications. There is a decrease in the quality of life of these patients. The aim was to study the relationship of the preoperative pain and trismus with the incidence of complications, as well as to investigate the quality of life. Materials and Methods: A prospective study on 60 patients with isolated mandibular fractures was performed, with a follow-up period of six months. The level of preoperative pain was measured on a 0–10 scale, while the mouth opening was measured with a caliper. All patients were treated surgically on the third day after the fracture. The University of Washington Quality of Life (UW-QOL v4) questionnaire was used to analyze the quality of life. Results: The most common types of complications were the occlusal derangement and facial asymmetry. The majority of complications were treated with counseling and physical therapy. The degree of preoperative pain was significantly positively related to the onset of complications (rs = 0.782, p = 0.004). The interincisal distance showed a significant inverse relation with the incidence of complications (rs = −0.722, p < 0.001). The patients regarded the pain, appearance and mood issues as the most important issues during the first postoperative month. Conclusions: The degree of inflammatory symptoms may be positively related to the onset of complications occurring after the rigid fixation of mandibular fractures. The postoperative health-related and overall quality of life was unsatisfactory in nearly half of the patients.
Microstructure, Mechanical Properties, and Constitutive Models for Ti–6Al–4V Alloy Fabricated by Selective Laser Melting (SLM)
Authors: Tao Pan , Zhong Jiangwei , Li Huaixue , Hu Quandong , Gong Shuili , Xu Qingyan
Published: April 2019

The mechanical performances and microstructure of Ti–6Al–4V built by selective laser melting were evaluated by optical microscopy, transmission electron microscopy, and room temperature tensile testing, and compared with the wrought and as-cast material. The flow behavior of the as-produced Ti–6Al–4V at temperatures varying from 700–900 °C at an interval of 50 °C and strain rates ranging from 10−2–101 s−1 was experimentally acquired. According to the experimental measurement, the Johnson–Cook, modified Arrhenius model, and artificial neural network were constructed. A comparative investigation on the predictability of established models was performed. The as-produced microstructure is made up of non-equilibrium martensite and columnar grains, leading to higher strength and lower ductility with respect to the conventional material. In room temperature tensile tests, the SLMed Ti–6Al–4V shows the characteristics of continuous yielding and unobvious work-hardening. The flow stress rapidly reaches the peak, and the softening rate depends on the strain rates and deformed temperatures in hot compression. The Johnson–Cook model could well predict the flow stress during quasi-static tensile deformation, but the model constants might vary with the process conditions. For dynamic compression, the artificial neural network exhibits higher accuracy to fit the flow stress of SLMed Ti–6Al–4V, and higher error to predict the conditions out of the model data, compared to the modified Arrhenius model involving the compensation of strain rate and strain.
Numerical Simulation of Solidification Behavior and Solute Transport in Slab Continuous Casting with S-EMS
Authors: Jiang Dongbin , Zhu Miaoyong , Zhang Lifeng
Published: April 2019

A 3D numerical model was built to investigate the transport phenomena in slab continuous casting process with secondary electromagnetic stirring (S-EMS). In the model, the columnar grain grew from strand surface and it should be treated as a porous media. While for the equiaxed zone, the nucleated grain moves with fluid flow in the earlier stage and it was regarded as a slurry. The model was validated by measured strand surface temperature and magnetic induction intensity. The results show that the solidification end near the 1/4 width of slab was postponed, due to the liquid flow from a submerged entry nozzle injected to the strand’s narrow face. As the linear stirring in the same direction is applied, liquid moves from side B to side A and then penetrates deep downward with higher temperature. In the later stage, the solidification end near the side A is postponed and the solute element is concentrated. When linear stirring in the opposite direction is used, the solidification end near the side A moves backward, while that near the side B moves forward. Moreover, it is found that the solute segregation in the side B is deteriorated, but that in the side A is reduced. As rotational stirring mode is applied, the evenness of solidification end profile is improved and the centerline segregation is reduced, especially with higher current intensity. Therefore, it is concluded that the linear stirring mode is not appropriated for slab casting, while the rotational stirring mode is more suitable.
Optimizing Conditions for Scandium Extraction from Bauxite Residue Using Taguchi Methodology
Authors: Lymperopoulou Theopisti , Georgiou Paraskevas , Tsakanika Lamprini-Areti , Hatzilyberis Konstantinos , Ochsenkuehn-Petropoulou Maria
Published: April 2019

Bauxite residue is the voluminous by-product of alumina production after Bayer process. Its high alkalinity causes disposal problems and harmful environmental impacts. However, the residue contains significant amounts of valuable elements, such as rare earth elements, including scandium. Greek bauxite residue contains a high amount of scandium close to its main resources. Taking into account scandium’s limited availability coupled with its high demand in modern technology, bauxite residue could be considered as a potential resource for scandium recovery. In this study, the optimization of scandium extraction from bauxite residue with sulfuric acid is investigated using Taguchi methodology. Based on previous studies, acid molarity, leaching time, solid/liquid ratio, and reaction temperature were selected as control parameters for the selective Sc recovery. Method optimization targeted the highest concentration of scandium combined with the lowest concentration of iron without taking into account application constraints. Maximization of scandium concentration can be achieved only by reduced selectivity. The predicted values resulted from the Taguchi methodology were affirmed by a confirmation experiment conducted at optimal conditions. Regression analysis provided the respective equations to be applied on several conditions, depending on different applications.
Development of Highly Selective 1,2,3-Triazole-containing Peptidic Polo-like Kinase 1 Polo-box Domain-binding Inhibitors
Authors: Zhao Xue Zhi , Tsuji Kohei , Hymel David , Burke Terrence R.
Published: April 2019

Members of the polo-like kinase (Plk) family of serine/threonine protein kinases play crucial roles in cell cycle regulation and proliferation. Of the five Plks (Plk1–5), Plk1 is recognized as an anticancer drug target. Plk1 contains multiple structural components that are important for its proper biological function. These include an N-terminal catalytic domain and a C-terminal non-catalytic polo-box domain (PBD). The PBD binds to phosphothreonine (pT) and phosphoserine-containing sequences. Blocking PBD-dependent interactions offers a potential means of down-regulating Plk1 function that is distinct from targeting its ATP-binding site. Previously, we demonstrated by tethering alkylphenyl chains from the N(π)-position of the His residue in the 5-mer PLHSpT, that we were able to access a hydrophobic “cryptic” binding pocket on the surface of the PBD, and in so doing enhance binding affinities by approximately 1000-fold. More recently, we optimized these PBD-ligand interactions using an oxime ligation-based strategy. Herein, using azide-alkyne cycloaddition reactions, we explore new triazole-containing PBD-binding antagonists. Some of these ligands retain the high PBD-binding affinity of the parent peptide, while showing desirable enhanced selectivity for the PBD of Plk1 relative to the PBDs of Plk2 and Plk3.
Renoprotective Effect of Laminaria japonica Polysaccharide in Adenine-Induced Chronic Renal Failure
Authors: Long Miao , Li Qiang-Ming , Fang Qing , Pan Li-Hua , Zha Xue-Qiang , Luo Jian-Ping
Published: April 2019

Chronic renal failure (CRF) is a major public health problem worldwide. In this work, we investigated the effects of a purified Laminaria japonica polysaccharide (LJP61A) on renal function using an adenine-induced CRF mice model. Results exhibited that adenine treatment caused serious renal pathological damages and elevation of serum creatinine and blood urea nitrogen of mice. However, these changes could be significantly reversed by the administration of LJP61A in a dose-dependent manner. Additionally, LJP61A could dramatically reduce weight loss, improve the urine biochemical index, and regulate the electrolyte disturbance of CRF mice. These results suggest that the renal function of adenine-induced CRF mice can be improved by LJP61A, which might be developed into a potential therapeutic agent for CRF patients.
Tri-Cyclic Nucleobase Analogs and Their Ribosides as Substrates of Purine-Nucleoside Phosphorylases. II Guanine and Isoguanine Derivatives
Authors: Stachelska-Wierzchowska Alicja , Wierzchowski Jacek , Górka Michał , Bzowska Agnieszka , Wielgus-Kutrowska Beata
Published: April 2019

Etheno-derivatives of guanine, O6-methylguanine, and isoguanine were prepared and purified using standard methods. The title compounds were examined as potential substrates of purine-nucleoside phosphorylases from various sources in the reverse (synthetic) pathway. It was found that 1,N2-etheno-guanine and 1,N6-etheno-isoguanine are excellent substrates for purine-nucleoside phosphorylase (PNP) from E. coli, while O6-methyl-N2,3-etheno-guanine exhibited moderate activity vs. this enzyme. The latter two compounds displayed intense fluorescence in neutral aqueous medium, and so did the corresponding ribosylation products. By contrast, PNP from calf spleens exhibited only modest activity towards 1,N6-etheno-isoguanine; the remaining compounds were not ribosylated by this enzyme. The enzymatic ribosylation of 1,N6-etheno-isoguanine using two forms of calf PNP (wild type and N243D) and E. coli PNP (wild type and D204N) gave three different products, which were identified on the basis of NMR analysis and comparison with the product of the isoguanosine reaction with chloroacetic aldehyde, which gave an essentially single compound, identified unequivocally as N9-riboside. With the wild-type E. coli enzyme as a catalyst, N9-β-d- and N7-β-d-ribosides are obtained in proportion ~1:3, while calf PNP produced another riboside, tentatively identified as N6-β-d-riboside. The potential application of various forms of PNP for synthesis of the tri-cyclic nucleoside analogs is discussed.
Formulation of Ethyl Cellulose Microparticles Incorporated Pheophytin A Isolated from Suaeda vermiculata for Antioxidant and Cytotoxic Activities
Authors: Mohammed Hamdoon A. , Al-Omar Mohsen S. , El-Readi Mahmoud Zaki , Alhowail Ahmad H. , Aldubayan Maha A. , Abdellatif Ahmed A. H.
Published: April 2019

Background: This study is designed to discover a method for delivering an efficient potent pheophytin a (pheo-a) into more absorbed and small polymeric ethyl cellulose (EC) microparticles. Methods: Silica gel and Sephadex LH-20 columns were used to isolate pheo-a from the chloroform extract of the edible plant, Suaeda vermiculata. Pheo-a was incorporated into EC microparticles using emulsion-solvent techniques. The antioxidant activity of pheo-a microparticles was confirmed by the level of superoxide radical (SOD), nitric oxide (NO), and reducing power (RP) methods. Meanwhile, the cytotoxic effect of the product was investigated on MCF-7 cells using MTT assay. Results: Pheo-a was isolated from S. vermiculata in a 12% concentration of the total chloroform extract. The structures were confirmed by NMR and IR spectroscopic analysis. The formulated microparticles were uniform, completely dispersed in the aqueous media, compatible as ingredients, and had a mean diameter of 139 ± 1.56 µm as measured by a particle size analyzer. Pheo-a demonstrated a valuable antioxidant activity when compared with ascorbic acid. The IC50 values of pheo-a microparticles were 200.5 and 137.7 µg/mL for SOD, and NO respectively. The reducing power of pheo-a microparticles was more potent than ascorbic acid and had a 4.2 µg/mL for IC50 value. Pheo-a microparticles did not show notable cytotoxicity on the MCF-7 cell line (IC50 = 35.9 µg/mL) compared with doxorubicin (IC50 = 3.2 µg/mL). Conclusions: the results showed that water-soluble pheo-a microparticles were prepared with a valuable antioxidant activity in a wide range of concentrations with a noteworthy cytotoxic effect.
QuinoxalineTacrine QT78, a Cholinesterase Inhibitor as a Potential Ligand for Alzheimer’s Disease Therapy
Authors: Ramos Eva , Palomino-Antolín Alejandra , Bartolini Manuela , Iriepa Isabel , Moraleda Ignacio , Diez-Iriepa Daniel , Samadi Abdelouahid , Cortina Carol V. , Chioua Mourad , Egea Javier , Romero Alejandro , Marco-Contelles José
Published: April 2019

We report the synthesis and relevant pharmacological properties of the quinoxalinetacrine (QT) hybrid QT78 in a project targeted to identify new non-hepatotoxic tacrine derivatives for Alzheimer’s disease therapy. We have found that QT78 is less toxic than tacrine at high concentrations (from 100 μM to 1 mM), less potent than tacrine as a ChE inhibitor, but shows selective BuChE inhibition (IC50 (hAChE) = 22.0 ± 1.3 μM; IC50 (hBuChE) = 6.79 ± 0.33 μM). Moreover, QT78 showed effective and strong neuroprotection against diverse toxic stimuli, such as rotenone plus oligomycin-A or okadaic acid, of biological significance for Alzheimer’s disease.
Dehydrocostus Lactone Suppresses LPS-induced Acute Lung Injury and Macrophage Activation through NF-κB Signaling Pathway Mediated by p38 MAPK and Akt
Authors: Nie Yunjuan , Wang Zhongxuan , Chai Gaoshang , Xiong Yue , Li Boyu , Zhang Hui , Xin Ruiting , Qian Xiaohang , Tang Zihan , Wu Jiajun , Zhao Peng
Published: April 2019

Acute lung injury (ALI) is a severe clinical disease marked by dysregulated inflammation response and has a high rate of morbidity and mortality. Macrophages, which play diverse roles in the inflammatory response, are becoming therapeutic targets in ALI. In this study we investigated the effects of dehydrocostus lactone (DHL), a natural sesquiterpene, on macrophage activation and LPS-induced ALI. The macrophage cell line RAW264.7 and primary lung macrophages were incubated with DHL (0, 3, 5, 10 and 30 μmol/L) for 0.5 h and then challenged with LPS (100 ng/mL) for up to 8 hours. C57BL/6 mice were intratracheally injected with LPS (5 mg/kg) to induce acute lung injury (ALI) and then treated with a range of DHL doses intraperitoneally (5 to 20 mg/kg). The results showed that DHL inhibited LPS-induced production of proinflammatory mediators such as iNOS, NO, and cytokines including TNF-α, IL-6, IL-1β, and IL-12 p35 by suppressing the activity of NF-κB via p38 MAPK/MK2 and Akt signaling pathway in macrophages. The in vivo results revealed that DHL significantly attenuated LPS-induced pathological injury and reduced cytokines expression in the lung. NF-κB, p38 MAPK/MK2 and Akt signaling molecules were also involved in the anti-inflammatory effect. Collectively, our findings suggested that DHL is a promising agent for alleviating LPS-induced ALI.
Overexpression of the Melatonin Synthesis-Related Gene SlCOMT1 Improves the Resistance of Tomato to Salt Stress
Authors: Liu Dan-Dan , Sun Xiao-Shuai , Liu Lin , Shi Hong-Di , Chen Sui-Yun , Zhao Da-Ke
Published: April 2019

Melatonin can increase plant resistance to stress, and exogenous melatonin has been reported to promote stress resistance in plants. In this study, a melatonin biosynthesis-related SlCOMT1 gene was cloned from tomato (Solanum lycopersicum Mill. cv. Ailsa Craig), which is highly expressed in fruits compared with other organs. The protein was found to locate in the cytoplasm. Melatonin content in SlCOMT1 overexpression transgenic tomato plants was significantly higher than that in wild-type plants. Under 800 mM NaCl stress, the transcript level of SlCOMT1 in tomato leaf was positively related to the melatonin contents. Furthermore, compared with that in wild-type plants, levels of superoxide and hydrogen peroxide were lower while the content of proline was higher in SlCOMT1 transgenic tomatoes. Therefore, SlCOMT1 was closely associated with melatonin biosynthesis confers the significant salt tolerance, providing a clue to cope with the growing global problem of salination in agricultural production.
Zeolite Clinoptilolite: Therapeutic Virtues of an Ancient Mineral
Authors: Mastinu Andrea , Kumar Amit , Maccarinelli Giuseppina , Bonini Sara Anna , Premoli Marika , Aria Francesca , Gianoncelli Alessandra , Memo Maurizio
Published: April 2019

Zeolites are porous minerals with high absorbency and ion-exchange capacity. Their molecular structure is a dense network of AlO4 and SiO4 that generates cavities where water and other polar molecules or ions are inserted/exchanged. Even though there are several synthetic or natural occurring species of zeolites, the most widespread and studied is the naturally occurring zeolite clinoptilolite (ZC). ZC is an excellent detoxifying, antioxidant and anti-inflammatory agent. As a result, it is been used in many industrial applications ranging from environmental remediation to oral applications/supplementation in vivo in humans as food supplements or medical devices. Moreover, the modification as micronization of ZC (M-ZC) or tribomechanically activated zeolite clinoptilolite (TMAZ) or furthermore as double tribomechanically activated zeolite clinoptilolite (PMA-ZC) allows improving its benefits in preclinical and clinical models. Despite its extensive use, many underlying action mechanisms of ZC in its natural or modified forms are still unclear, especially in humans. The main aim of this review is to shed light on the geochemical aspects and therapeutic potentials of ZC with a vision of endorsing further preclinical and clinical research on zeolites, in specific on the ZC and its modified forms as a potential agent for promoting human brain health and overall well-being.
Effect of Thickness of Molybdenum Nano-Interlayer on Cohesion between Molybdenum/Titanium Multilayer Film and Silicon Substrate
Authors: Shen Huahai , Yao Bing , Zhang Jianwei , Zhu Xinqiao , Xiang Xia , Zhou Xiaosong , Zu Xiaotao
Published: April 2019

Titanium (Ti) film has been used as a hydrogen storage material. The effect of the thickness of a molybdenum (Mo) nano-interlayer on the cohesive strength between a Mo/Ti multilayer film and a single crystal silicon (Si) substrate was investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM), and nano-indenter. Four groups of Si/Mo/Ti multilayer films with different thicknesses of Mo and Ti films were fabricated. The XRD results showed that the introduction of the Mo layer suppressed the chemical reaction between the Ti film and Si substrate. The nano-indenter scratch results demonstrated that the cohesion between the Mo/Ti film and Si substrate decreased significantly with increasing Mo interlayer thickness. The XRD stress analysis indicated that the residual stress in the Si/Mo/Ti film was in-plane tensile stress which might be due to the lattice expansion at a high film growth temperature of 700 °C and the discrepancy of the thermal expansion coefficient between the Ti film and Si substrate. The tensile stress in the Si/Mo/Ti film decreased with increasing Mo interlayer thickness. During the cooling of the Si substrate, a greater decrease in tensile stress occurred for the thicker Mo interlayer sample, which became the driving force for reducing the cohesion between the Mo/Ti film and Si substrate. The results confirmed that the design of the Mo interlayer played an important role in the quality of the Ti film grown on Si substrate.
Effects of Particle Size and Extraction Methods on Cocoa Bean Shell Functional Beverage
Authors: Rojo-Poveda Olga , Barbosa-Pereira Letricia , Mateus-Reguengo Lívia , Bertolino Marta , Stévigny Caroline , Zeppa Giuseppe
Published: April 2019

One of the main by-products in cocoa industry is the cocoa bean shell (CBS), which represents approximately 12–20% of the bean. This product has been suggested as a food ingredient because of its aroma and high dietary fiber and polyphenol contents. The purpose of this work was to evaluate the effects of the CBS particle size and extraction methods on the chemical composition and consumer acceptance of a functional beverage, in order to find the best combination of technological parameters and health benefits. Five particle sizes of CBS powder and six home techniques were used for beverage preparation. The influence of these factors on the physico-chemical characteristics, methylxanthine and polyphenolic contents, antioxidant and antidiabetic properties, and consumer acceptance was evaluated. Total phenolic content values up to 1803.83 mg GAE/L were obtained for the beverages. Phenolic compounds and methylxanthines were identified and quantified by HPLC-PDA. These compounds may be related to the high antioxidant capacity (up to 7.29 mmol TE/L) and antidiabetic properties (up to 52.0% of α-glucosidase inhibition) observed. Furthermore, the consumer acceptance results indicated that CBS may represent an interesting ingredient for new functional beverages with potential health benefits, reducing the environmental and economic impact of by-product disposal.
Gellan Gum/Laponite Beads for the Modified Release of Drugs: Experimental and Modeling Study of Gastrointestinal Release
Authors: Adrover Alessandra , Paolicelli Patrizia , Petralito Stefania , Di Muzio Laura , Trilli Jordan , Cesa Stefania , Tho Ingunn , Casadei Maria Antonietta
Published: April 2019

In this study, gellan gum (GG), a natural polysaccharide, was used to fabricate spherical porous beads suitable as sustained drug delivery systems for oral administration. GG was cross-linked with calcium ions to prepare polymeric beads. Rheological studies and preliminary experiments of beads preparation allowed to identify the GG and the CaCl2 concentrations suitable for obtaining stable and spherical particles. GG beads were formed, through ionotropic gelation technique, with and without the presence of the synthetic clay laponite. The resultant beads were analyzed for dimensions (before and after freeze-drying), morphological aspects and ability to swell in different media miming biological fluids, namely SGF (Simulated Gastric Fluid, HCl 0.1 M) and SIF (Simulated Intestinal Fluid, phosphate buffer, 0.044 M, pH 7.4). The swelling degree was lower in SGF than in SIF and further reduced in the presence of laponite. The GG and GG-layered silicate composite beads were loaded with two model drugs having different molecular weight, namely theophylline and cyanocobalamin (vitamin B12) and subjected to in-vitro release studies in SGF and SIF. The presence of laponite in the bead formulation increased the drug entrapment efficiency and slowed-down the release kinetics of both drugs in the gastric environment. A moving-boundary swelling model with “diffuse” glassy-rubbery interface was proposed in order to describe the swelling behavior of porous freeze-dried beads. Consistently with the swelling model adopted, two moving-boundary drug release models were developed to interpret release data from highly porous beads of different drugs: drug molecules, e.g., theophylline, that exhibit a typical Fickian behavior of release curves and drugs, such as vitamin B12, whose release curves are affected by the physical/chemical interaction of the drug with the polymer/clay complex. Theoretical results support the experimental observations, thus confirming that laponite may be an effective additive for fabricating sustained drug delivery systems.
Effect of Selenium Biofortification and Beneficial Microorganism Inoculation on Yield, Quality and Antioxidant Properties of Shallot Bulbs
Authors: Golubkina Nadezhda , Zamana Svetlana , Seredin Timofei , Poluboyarinov Pavel , Sokolov Sergei , Baranova Helene , Krivenkov Leonid , Pietrantonio Laura , Caruso Gianluca
Published: April 2019

Plant biofortification with selenium in interaction with the application of an arbuscular mycorrhizal fungi (AMF)-based formulate, with the goal of enhancing Se bioavailability, is beneficial for the development of the environmentally friendly production of functional food with a high content of this microelement. Research was carried out in order to assess the effects of an AMF-based formulate and a non-inoculated control in factorial combination with two selenium treatments with an organic (selenocystine) or inorganic form (sodium selenate) and a non-treated control on the yield, quality, antioxidant properties, and elemental composition of shallot (Allium cepa L. Aggregatum group). Selenocystine showed the best effect on the growth and yield of mycorrhized plants, whereas sodium selenate was the most effective on the non-inoculated plants. The soluble solids, total sugars, monosaccharides, titratable acidity, and proteins attained higher values upon AMF inoculation. Sodium selenate resulted in higher soluble solids, total sugars and monosaccharide content, and titratable acidity than the non-treated control, and it also resulted in higher monosaccharides when compared to selenocystine; the latter showed higher protein content than the control. Calcium, Na, S, and Cl bulb concentrations were higher in the plants inoculated with the beneficial microorganisms. Calcium and sodium concentrations were higher in the bulbs of plants treated with both the selenium forms than in the control. Selenocystine-treated plants showed enhanced accumulation of sulfur and chlorine compared to the untreated plants. The AMF inoculation increased the bulb selenium content by 530%, and the Se biofortification with selenocystine and sodium selenate increased this value by 36% and 21%, respectively, compared to control plants. The AMF-based formulate led to increases in ascorbic acid and antioxidant activity when compared to the non-inoculated control. The bulb ascorbic acid was increased by fortification with both selenium forms when compared to the non-treated control. The results of our investigation showed that both AMF and selenium application represent environmentally friendly strategies to enhance the overall yield and quality performances of shallot bulbs, as well as their selenium content.
Structure and Flame-Retardant Actions of Rigid Polyurethane Foams with Expandable Graphite
Authors: Chen Yongjun , Luo Yuanfang , Guo Xiaohui , Chen Lijuan , Xu Tiwen , Jia Demin
Published: April 2019

In this paper, rigid polyurethane foams that were filled with expandable graphite (RPUF/EG) composites were prepared by the liquid blending method, and then the structure and flame retardancy performance of materials were investigated through optical microscope, scanning electron microscope, limit oxygen index, cone calorimeter, thermogravimetric analysis coupled to fourier transform infrared spectrum, and X-ray photoelectron spectroscopy. The results showed that a large number of EG could be good to the exhibition of flame retardancy of RPUF, where the optimal material was found at loading 15 phr EG that showed an increased limit oxygen index value and a decreased calorific or fuming value. TGA coupled FTIR and XPS revealed that EG could disassembled before RPUF under heating treatment, and it could form a pyknotic and enahnced residual carbon layer on RPUF surface after the fire, which restricted the transfer of gas, like oxygen or heat into PU matrix, finally resulting in the promotion of flame retardancy of RPUF.
One Step Further in the Characterization of Synthetic Polymers by Ion Mobility Mass Spectrometry: Evaluating the Contribution of End-groups
Authors: Duez Quentin , Liénard Romain , Moins Sébastien , Lemaur Vincent , Coulembier Olivier , Cornil Jérôme , Gerbaux Pascal , De Winter Julien
Published: April 2019

Several families of polymers possessing various end-groups are characterized by ion mobility mass spectrometry (IMMS). A significant contribution of the end-groups to the ion collision cross section (CCS) is observed, although their role is neglected in current fitting models described in literature. Comparing polymers prepared from different synthetic procedures might thus, be misleading with the current theoretical treatments. We show that this issue is alleviated by comparing the CCS of various polymer ions (polyesters and polyethers) as a function of the number of atoms in the macroion instead of the usual representation involving the degree of polymerization. Finally, we extract the atom number density from the spectra which gives us the possibility to evaluate the compaction of polymer ions, and by extension to discern isomeric polymers.
Polysaccharide Coating of Gelatin Gels for Controlled BSA Release
Authors: Gonzalez Jimena S. , Mijangos Carmen , Hernandez Rebeca
Published: April 2019

Self-assembly of natural polymers constitute a powerful route for the development of functional materials. In particular, layer-by-layer (LBL) assembly constitutes a versatile technique for the nanostructuration of biobased polymers into multilayer films. Gelatin has gained much attention for its abundance, biodegradability, and excellent gel-forming properties. However, gelatin gels melt at low temperature, thus limiting its practical application. With respect to the above considerations, here, we explored the potential application of gelatin gels as a matrix for protein delivery at physiological temperature. A model protein, bovine serum albumin (BSA), was encapsulated within gelatin gels and then coated with a different number of bilayers of alginate and chitosan (10, 25, 50) in order to modify the diffusion barrier. The coated gel samples were analyzed by means of Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) and confocal Raman spectroscopy, and it was found that the multilayer coatings onto polymer film were interpenetrated to some extent within the gelatin. The obtained results inferred that the coating of gelatin gels with polysaccharide multilayer film increased the thermal stability of gelatin gels and modulated the BSA release. Finally, the influence of a number of bilayers onto the drug release mechanism was determined. The Ritger-Peppas model was found to be the most accurate to describe the diffusion mechanism.
Exploring Adsorption Process of Lead (II) and Chromium (VI) Ions from Aqueous Solutions on Acid Activated Carbon Prepared from Juniperus procera Leaves
Authors: Ali Ismat H. , Al Mesfer Mohammed K. , Khan Mohammad I. , Danish Mohd , Alghamdi Majed M.
Published: April 2019

The adsorption potential of acid activated carbon prepared from leaves of Juniperus procera to remove Pb(II) and Cr(VI) toxic ions from aqueous solutions was investigated. The effects of solution pH, adsorbent mass, contact time, initial ion concentration and temperature on the biosorption process were studied, and the optimum conditions were determined. Moreover, Langmuir, Freundlich, Temkin and Dubinin–Radushkevich adsorption isotherm models were applied to analyze adsorption data. Thermodynamic parameters for the adsorption processes were calculated. Adsorption was found to be a spontaneous and endothermic process. In addition, kinetic studies revealed a pseudo-first order kinetics biosorption process. The obtained results suggest that acid activated Juniperus procera leaves powder can be used as a cheap, efficient and environmentally friendly adsorbent material with high removal efficiency up to 98% for Pb(II) and 96% for Cr(VI) at 0.80 and 1.00 g/100 mL, respectively. The duration of the process was 100 min and 120 min for Pb(II) and Cr(VI) ions, respectively. The morphology of the of prepared activated carbon was investigated by scanning electron microscope (SEM).