bluuebunny/crossref_metadata_2025 huggingface dataset:
Open Innovation for Sustainability or Not: Literature Reviews of Global Research Trends
Authors: Payán-Sánchez Belén , Belmonte-Ureña Luis Jesús , Plaza-Úbeda José Antonio , Vazquez-Brust Diego , Yakovleva Natalia , Pérez-Valls Miguel
Published: January 2021

The demand for innovative approaches applied to productive sectors is a reality present in the circular economy and open innovation is a relatively new concept that has revolutionized the literature about innovation management. Since the concept appeared in 2003, many articles have focused on its development and application. Although some studies have connected open innovation with sustainability, the relevance of this current on the global literature about open innovation is still unidentified. In this context, this paper tries to cover this gap with a bibliometric analysis focused on the evolution of the open innovation paradigm and the relevance of sustainability in this field of research. A sample of 3087 papers published between 2003 and 2019 in the Scopus database was obtained. The analysis revealed the main topics and the most prolific journals, authors, institutions, and countries, in terms of productivity, citations, and h-indexes. Besides of these contributions, keywords analysis reveals that, in recent years, sustainability and ecosystems are decisive variables in open innovation research.
Urban Planning and European Innovation Policy: Achieving Sustainability, Social Inclusion, and Economic Growth?
Authors: von Schönfeld Kim Carlotta , Ferreira António
Published: January 2021

Innovation has become a guiding principle for European Union policy. Funding schemes, research, and planning across all Member States are expected to be innovative. This article provides a critical analysis of the drivers and effects of this evolution. While positive results have been achieved due to innovation policies, this article proposes that taking a wider critical perspective reveals important caveats. The article zooms in on the EU’s innovation policies by analysing policy documents, projects funded, and on-the-ground impact on three citizen initiatives. The analysis asks whether and how the EU’s self-set goals of sustainability, social inclusion, and economic growth are approached and met in them. The findings suggest a problematic funnelling process. First, an emphasis on innovation is created with the objective of systematically unblocking resistance to the development and implementation of novelties in the name of competitiveness, job creation, and economic growth. Second, the idea of innovation is very loosely defined, while, when translated into urban planning, it is interpreted narrowly in terms of efficiency and behavioural change, digitalization, and smart technologies. As a result, (narrowly defined) innovation-led economic growth begins to supersede alternative values and visions for the future of European cities and regions. This can represent a problem for EU Member States as it creates a very limited, risk-based, and divisive direction of development. To contribute to the (re-)establishment of alternatives, this article finally offers policy recommendations primarily concerned with the reinstatement of the public interest beyond innovation-centred planning perspectives.
Ecosystem-Based Adaptation Practices as a Nature-Based Solution to Promote Water-Energy-Food Nexus Balance
Authors: Muthee Kennedy , Duguma Lalisa , Nzyoka Judith , Minang Peter
Published: January 2021

The objective of this study is to evaluate the contributions of ecosystem-based adaptation (EbA) practices to the water–energy–food (WEF) nexus balance, design practical pathways, and analyze barriers towards achievement of EbA-WEF balance. An area case study and descriptive methods were used to analyze data collected from 50 community forests (CFs) spread across three regions in The Gambia. Extensive information from relevant literature sources was also referred to in this study. Fourteen priority EbA practices were established and categorized into four major groups based on their application similarities. Among the anticipated ecosystem services were enhanced water resource conservation, food and feed production, enhanced energy supply, and improved community livelihoods to enhance their resilience. Pathways on how each practice under the broad category contributes to water, energy, and food were developed to demonstrate how they individually and collectively contribute towards the nexus balance. Key enablers identified included a conducive policy framework, institutional support, diverse incentives, information, knowledge, and technology transfer, and climate and non-climate barriers were cited as impediments. The paper concludes by outlining recommendations to overcome the established barriers.
The Evaluation System of the Sustainable Development of Municipal Solid Waste Landfills and Its Application
Authors: Liu Hailong , Luo Xiang , Jiang Xingyao , Cui Chunyi , Huyan Zhen
Published: January 2021

Improving the understanding of the stabilization process is of great significance to guide the sustainable development of municipal solid waste (MSW) landfills. An evaluation system of the stabilization process of MSW landfills has been established. The indices of the evaluation system involve the degradation degree of MSW, the release of landfill gas production potential, and the settlement of landfills. Based on the biochemical-consolidation-solute migration coupled model, an evaluation method of the MSW landfill stabilization process is proposed by combining field tests with numerical simulation. The stabilization process of the Jiangcungou landfill in China is investigated by using the proposed method. The analyzed results show that the stabilization process of high kitchen waste content landfills can be divided into three stages, which is different from the stabilization process of landfills in developed countries. For the Jiangcungou landfill, the ratio of cellulose to lignin in MSW decreases rapidly during the fast degradation stage when obvious settlement occurs. During the slow degradation stage, the hydrolysis rate is slow and settlement develops slowly. When the landfill reaches the stabilization stage, the ratio of cellulose to lignin of MSW changes very slowly; most of the landfill gas potential has been released; the settlement stabilization is completed basically. The change processes of the three evaluation indices are different, of which the degradation stabilization index is the main one. According to the findings above, leachate recirculation is recommended to adjust the degradation environment in the landfill, which can be helpful to avoid acidification at the fast degradation stage. Temporary cover is suggested to improve landfill gas collection efficiency at the beginning of the stable methanogenic stage. The landfill site closure should be operated when the settlement rate is low.
Moving Away from Category Exclusivity Deals to Sponsorship Activation Platforms: The Case of the Ryder Cup
Authors: Bai Yunchao , Yim Brian H. , Breedlove John , Zhang James J.
Published: January 2021

As a biennial event, the Ryder Cup is a men’s golf competition between teams from Europe and the United States. Ernst & Young (EY) and Standard Life Investments (SLI), who are in same business category (i.e., financial services), have served as official partners of the event in recent years. While the two firms are willing to move away from the traditional sponsorship practices of category exclusivity deals, both have been able to achieve significant success through their collaborative efforts in activating their sponsorships. This is a new, fascinating phenomenon in both sponsorship concept and practice. Through an exploratory inductive inquiry process, in this study we conduct a case analysis by examining the sponsorship activations of EY and SLI at the 2014 Ryder Cup event held in the UK. The findings demonstrate that social media plays an impactful role in the companies’ ability to engage target audiences. EY used the Ryder Cup captain as a brand ambassador, who embodied its sponsorship theme of leadership and teamwork. SLI focused on running advertising campaigns to build company image and increase brand awareness.
How to Measure Sustainable Housing: A Proposal for an Indicator-Based Assessment Tool
Authors: Adamec Jakub , Janoušková Svatava , Hák Tomáš
Published: January 2021

Housing drives urban development and has a significant potential for contributing to sustainability. However, ample sustainability indicator sets fail to include relevant indicators of sustainable housing—assessment seems to be an underdeveloped topic. We chose the United Nations Geneva Charter on Sustainable Housing as a conceptual foundation for the proposed assessment tool. It addresses recent challenges by four defining principles and related rationales, thus forming a theoretical basis of sustainable housing. We applied both theoretical research (desk-top analysis) and qualitative research (an expert panel) to develop a comprehensive framework for sustainable housing and complemented it with relevant indicators. The proposed housing sustainability assessment tool (HSAT) explicitly advocates a holistic approach that seeks to balance the environmental, social, economic and institutional dimensions of sustainability; simultaneously, it includes an integrated concept of the building–community–locality. Hence, this article does not seek to redefine the sustainable housing definition or concept but to contribute to the development of a highly relevant indicator-based system for its assessment. This will ensure that correct and unambiguous messages are sent not only to policymakers but also investors, urban planners and finally also the citizens—the housing clients.
Behavior of Online Prosumers in Organic Product Market as Determinant of Sustainable Consumption
Authors: Maciaszczyk Magdalena , Kocot Maria
Published: January 2021

The article focuses on the specificity of the behavior of modern prosumers in the market for organic products as a determinant of sustainable consumption. The strengthening of the pro-consumer relationship is made possible thanks to ongoing technological changes, especially the development of the Internet. It is thanks to our online activities and the opinions of other Internet users that pro-consumer decisions concerning online purchases are shaped. The article presents the results of empirical studies conducted in 2020 on a sample of 664 respondents using a questionnaire, while the survey results were developed using SPSS Statistics. Findings of research showed the willingness of consumers for prosumption and kind of behaviors that were most often among the research group. In the course of research proceedings, it was possible to define factors that dispose declarative prosumers for the purchase of organic products. Conducted analyses also allowed for building the model of the influence of individual characteristics of respondents on the index of prosumption.
Isospin Symmetry Breaking Effects on the Mass-Radius Relation of a Neutron Star
Authors: Selva Giovanni , Roca-Maza Xavier , Colò Gianluca
Published: January 2021

Isospin symmetry breaking effects on the mass-radius relation of a cold, non-accreting neutron star are studied on the basis of two Skyrme Energy Density Functionals (EDFs). One functional contains isospin symmetry breaking terms other than those typically included in Skyrme EDFs while its counterpart is of standard form. Both functionals are based on the same fitting protocol except for the observables and pseudo-observables sensitive to the isospin symmetry breaking channel. The quality of those functionals is similar in the description of terrestrial observables but choosing either of them has a non-negligible effect on the mass-radius relation and tidal deformability of a neutron star. Further investigations are needed to clarify the effects of isospin symmetry breaking on these and other observables of neutron stars that are, and will become, available.
Secure the IoT Networks as Epidemic Containment Game
Authors: Zhu Juntao , Ding Hong , Tao Yuchen , Wang Zhen , Yu Lanping
Published: January 2021

The spread of a computer virus among the Internet of Things (IoT) devices can be modeled as an Epidemic Containment (EC) game, where each owner decides the strategy, e.g., installing anti-virus software, to maximize his utility against the susceptible-infected-susceptible (SIS) model of the epidemics on graphs. The EC game’s canonical solution concepts are the Minimum/Maximum Nash Equilibria (MinNE/MaxNE). However, computing the exact MinNE/MaxNE is NP-hard, and only several heuristic algorithms are proposed to approximate the MinNE/MaxNE. To calculate the exact MinNE/MaxNE, we provide a thorough analysis of some special graphs and propose scalable and exact algorithms for general graphs. Especially, our contributions are four-fold. First, we analytically give the MinNE/MaxNE for EC on special graphs based on spectral radius. Second, we provide an integer linear programming formulation (ILP) to determine MinNE/MaxNE for the general graphs with the small epidemic threshold. Third, we propose a branch-and-bound (BnB) framework to compute the exact MinNE/MaxNE in the general graphs with several heuristic methods to branch the variables. Fourth, we adopt NetShiled (NetS) method to approximate the MinNE to improve the scalability. Extensive experiments demonstrate that our BnB algorithm can outperform the naive enumeration method in scalability, and the NetS can improve the scalability significantly and outperform the previous heuristic method in solution quality.
Symmetry Transformations in Metazoan Evolution and Development
Authors: Isaeva Valeria V. , Kasyanov Nickolay V.
Published: January 2021

In this review, we consider transformations of axial symmetry in metazoan evolution and development, the genetic basis, and phenotypic expressions of different axial body plans. In addition to the main symmetry types in metazoan body plans, such as rotation (radial symmetry), reflection (mirror and glide reflection symmetry), and translation (metamerism), many biological objects show scale (fractal) symmetry as well as some symmetry-type combinations. Some genetic mechanisms of axial pattern establishment, creating a coordinate system of a metazoan body plan, bilaterian segmentation, and left–right symmetry/asymmetry, are analysed. Data on the crucial contribution of coupled functions of the Wnt, BMP, Notch, and Hedgehog signaling pathways (all pathways are designated according to the abbreviated or full names of genes or their protein products; for details, see below) and the axial Hox-code in the formation and maintenance of metazoan body plans are necessary for an understanding of the evolutionary diversification and phenotypic expression of various types of axial symmetry. The lost body plans of some extinct Ediacaran and early Cambrian metazoans are also considered in comparison with axial body plans and posterior growth in living animals.
Phishing for (Quantum-Like) Phools—Theory and Experimental Evidence
Authors: Lambert-Mogiliansky Ariane , Calmettes Adrian
Published: January 2021

Quantum-like decision theory is by now a theoretically well-developed field (see e.g., Danilov, Lambert-Mogiliansky & Vergopoulos, 2018). We provide a first test of the predictions of an application of this approach to persuasion. One remarkable result entails that, in contrast to Bayesian persuasion, distraction rather than relevant information has a powerful potential to influence decision-making. We first develop a quantum decision model of choice between two uncertain alternatives. We derive the impact of persuasion by means of distractive questions and contrast them with the predictions of the Bayesian model. Next, we provide the results from a first test of the theory. We conducted an experiment where respondents choose between supporting either one of two projects to save endangered species. We tested the impact of persuasion in the form of questions related to different aspects of the uncertain value of the two projects. The experiment involved 1253 respondents divided into three groups: a control group, a first treatment group and the distraction treatment group. Our main result is that, in accordance with the predictions of quantum persuasion but in violation with the Bayesian model, distraction significantly affects decision-making. Population variables play no role. Some significant variations between subgroups are exhibited and discussed. The results of the experiment provide support for the hypothesis that the manipulability of people’s decision-making can to some extent be explained by the quantum indeterminacy of their subjective representation of reality.
Sublethal Effects of Chlorantraniliprole on Spodoptera litura (Lepidoptera: Noctuidae) Moth: Implication for Attract-And-Kill Strategy
Authors: Kong Fanfang , Song Yaqin , Zhang Qian , Wang Zhongyue , Liu Yongqiang
Published: January 2021

The integrated use of plant-derived volatile attractants and synthetic insecticides in attract-and-kill programs is a useful tool for integrated pest management programs reducing pesticide input. Efficient alternative insecticides are critically needed to replace methomyl, which has been banned on cruciferous vegetables in China because it is also highly toxic to nontarget organisms. In the present study, among 15 commonly used insecticides were screened for toxicity against S. litura moths, where chlorantraniliprole, flubendiamide, and emamectin benzoate was found to have the highest levels of toxicity (LC50 of 0.56, 3.85, and 6.03 mg a.i. L−1 respectively). After exposure to the low lethal concentration LC50 of chlorantraniliprole, fecundity of the moths was substantially reduced. Egg-hatching was lower for LC20- and LC50-treated moth pairs than for untreated control pairs. Net reproductive rate (R0), intrinsic rate of increase (r), and finite rate of increase (λ) were significantly reduced in LC50♀ × LC50♂ cohorts. Larval mortality was significantly higher in subsequent generations in pairs of LC50-treated moths. Chlorantraniliprole, which was most toxic and had significant sublethal effects on moths, can be used as an alternative insecticide to methomyl in the attracticide for controlling S. litura moths, and the LC50 indicated a high potential for efficacy in the control S. litura through attract-and-kill schemes.
Populations of Helicoverpa zea (Boddie) in the Southeastern United States are Commonly Resistant to Cry1Ab, but Still Susceptible to Vip3Aa20 Expressed in MIR 162 Corn
Authors: Niu Ying , Oyediran Isaac , Yu Wenbo , Lin Shucong , Dimase Marcelo , Brown Sebe , Reay-Jones Francis P. F. , Cook Don , Reisig Dominic , Thrash Ben , Ni Xinzhi , Paula-Moraes Silvana V. , Zhang Yan , Chen Jeng Shong , Wen Zhimou , Huang Fangneng
Published: January 2021

The corn earworm, Helicoverpa zea (Boddie), is a major pest targeted by pyramided Bacillus thuringiensis (Bt) corn and cotton in the U.S. Cry1Ab is one of the first insecticidal toxins used in Bt crops, while Vip3A is a relatively new toxin that has recently been incorporated into Cry corn with event MIR 162 and Cry cotton varieties to generate pyramided Bt traits targeting lepidopteran pests including H. zea. The objectives of this study were to determine the current status and distribution of the Cry1Ab resistance, and evaluate the susceptibility to Vip3Aa20 expressed in MIR 162 corn in H. zea in the southeastern U.S. During 2018 and 2019, 32 H. zea populations were collected from non-Bt corn (19 populations), Cry corn (12), and Cry/Vip3A cotton (1) across major corn areas in seven southeastern states of the U.S. Susceptibility of these populations to Cry1Ab and Vip3Aa20 was determined using diet-overlay bioassays. Compared to a known susceptible insect strain, 80% of the field populations were 13- to >150-fold resistant to Cry1Ab, while their response to Vip3Aa20 ranged from >11-fold more susceptible to 9-fold more tolerant. Mean susceptibility to each Bt toxin was not significantly different between the two groups of the populations collected from non-Bt and Bt crops, as well as between the two groups of the populations collected during 2018 and 2019. The results show that resistance to Cry1Ab in H. zea is widely distributed across the region. However, the Cry1Ab-resistant populations are not cross-resistant to Vip3Aa20, and H. zea in the region is still susceptible to the Vip3Aa20 toxin. Vip3Aa20 concentrations between 5 and 10 µg/cm2 may be used as diagnostic concentrations for susceptibility monitoring in future. Additional studies are necessary to elucidate the impact of the selection with Bt corn on resistance evolution in H. zea to Vip3A cotton in the U.S.
The Battle between Retroviruses and APOBEC3 Genes: Its Past and Present
Authors: Uriu Keiya , Kosugi Yusuke , Ito Jumpei , Sato Kei
Published: January 2021

The APOBEC3 family of proteins in mammals consists of cellular cytosine deaminases and well-known restriction factors against retroviruses, including lentiviruses. APOBEC3 genes are highly amplified and diversified in mammals, suggesting that their evolution and diversification have been driven by conflicts with ancient viruses. At present, lentiviruses, including HIV, the causative agent of AIDS, are known to encode a viral protein called Vif to overcome the antiviral effects of the APOBEC3 proteins of their hosts. Recent studies have revealed that the acquisition of an anti-APOBEC3 ability by lentiviruses is a key step in achieving successful cross-species transmission. Here, we summarize the current knowledge of the interplay between mammalian APOBEC3 proteins and viral infections and introduce a scenario of the coevolution of mammalian APOBEC3 genes and viruses.
Formation and Function of Liquid-Like Viral Factories in Negative-Sense Single-Stranded RNA Virus Infections
Authors: Su Justin , Wilson Maxwell , Samuel Charles , Ma Dzwokai
Published: January 2021

Liquid–liquid phase separation (LLPS) represents a major physiochemical principle to organize intracellular membrane-less structures. Studies with non-segmented negative-sense (NNS) RNA viruses have uncovered a key role of LLPS in the formation of viral inclusion bodies (IBs), sites of viral protein concentration in the cytoplasm of infected cells. These studies further reveal the structural and functional complexity of viral IB factories and provide a foundation for their future research. Herein, we review the literature leading to the discovery of LLPS-driven formation of IBs in NNS RNA virus-infected cells and the identification of viral scaffold components involved, and then outline important questions and challenges for IB assembly and disassembly. We discuss the functional implications of LLPS in the life cycle of NNS RNA viruses and host responses to infection. Finally, we speculate on the potential mechanisms underlying IB maturation, a phenomenon relevant to many human diseases.
Structural Mapping of Mutations in Spike, RdRp and Orf3a Genes of SARS-CoV-2 in Influenza Like Illness (ILI) Patients
Authors: Alosaimi Bandar , Naeem Asif , Alghoribi Majed F. , Okdah Lilian , Hamed Maaweya E. , AlYami Ahmad S. , Alotaibi Athari , Enani Mushira
Published: January 2021

In December 2019, the emergence of SARS-CoV-2 virus in China led to a pandemic. Since both Influenza Like Illness (ILI) and COVID-19 case definitions overlap, we re-investigated the ILI cases using PCR for the presence of SARS-CoV-2 in 739 nasopharyngeal swabs collected from November 2019 to March 2020. SARS-CoV-2 RNA was found in 37 samples (5%) collected mostly during February 2020. It was followed by confirmation of evolutionary and spatial relationships using next generation sequencing (NGS). We observed that the overall incidence of ILI cases during 2019–2020 influenza season was considerably higher than previous years and was gradually replaced with SARS-CoV-2, which indicated a silent transmission among ambulatory patients. Sequencing of representative isolates confirmed independent introductions and silent transmission earlier than previously thought. Evolutionary and spatial analyses revealed clustering in the GH clade, characterized by three amino acid substitutions in spike gene (D614G), RdRp (P323L) and NS3 (Q57H). P323L causes conformational change near nsp8 binding site that might affect virus replication and transcription. In conclusion, assessment of the community transmission among patients with mild COVID-19 illness, particularly those without epidemiological link for acquiring the virus, is of utmost importance to guide policy makers to optimize public health interventions. The detection of SARS-CoV-2 in ILI cases shows the importance of ILI surveillance systems and warrants its further strengthening to mitigate the ongoing transmission of SARS-CoV-2. The effect of NS3 substitutions on oligomerization or membrane channel function (intra- and extracellular) needs functional validation.
Novel Flavivirus Attenuation Markers Identified in the Envelope Protein of Alfuy Virus
Authors: Westlake Daniel , Bielefeldt-Ohmann Helle , Prow Natalie A. , Hall Roy A.
Published: January 2021

Alfuy (ALFV) is an attenuated flavivirus related to the Murray Valley encephalitis virus (MVEV). We previously identified markers of attenuation in the envelope (E) protein of the prototype strain (ALFV3929), including the hinge region (E273–277) and lack of glycosylation at E154-156. To further determine the mechanisms of attenuation we assessed ALFV3929 binding to glycosaminoglycans (GAG), a known mechanism of flaviviruses attenuation. Indeed, ALFV3929 exhibited reduced binding to GAG-rich cells in the presence of heparin; however, low-passage ALFV isolates were relatively unaffected. Sequence comparisons between ALFV strains and structural modelling incriminated a positively-charged residue (K327) in ALFV3929 as a GAG-binding motif. Substitution of this residue to the corresponding uncharged residue in MVEV (L), using a previously described chimeric virus containing the prM & E genes of ALFV3929 in the backbone of MVEV (MVEV/ALFV-prME), confirmed a role for K327 in enhanced GAG binding. When the wild type residues at E327, E273–277 and E154–156 of ALFV3929 were replaced with the corresponding residues from virulent MVEV, it revealed each motif contributed to attenuation of ALFV3929, with the E327/E273–277 combination most dominant. These data demonstrate that attenuation of ALFV3929 is multifactorial and provide new insights for the rational design of attenuated flavivirus vaccines.
Zika RNA and Flavivirus-Like Antigens in the Sperm Cells of Symptomatic and Asymptomatic Subjects
Authors: Vanegas Hernan , González Fredman , Reyes Yaoska , Centeno Edwing , Palacios Jayrintzina , Zepeda Omar , Hagbom Marie , Collins Matthew H. , Coward R. Matthew , Becker-Dreps Sylvia , Bowman Natalie , Bucardo Filemón
Published: January 2021

Zika virus (ZIKV) RNA has been found to remain in human semen for up to one year after infection, but the presence of Flavivirus antigens in the different compartments of semen has been largely unexplored. Following the introduction of ZIKV in Nicaragua (2016), a prospective study of patients with clinical symptoms consistent with ZIKV was conducted in León to investigate virus shedding in different fluids. ZIKV infection was confirmed in 16 male subjects (≥18 years of age) by RT-qPCR in either blood, saliva or urine. Of these, three provided semen samples at 7, 14, 21, 28, 60 and 180 days postsymptom onset (DPSO) for Flavivirus antigens and RNA studies. These cases were compared with 19 asymptomatic controls. Flavivirus antigens were examined by immunofluorescence (IF) using the 4G2 Mabs, and confocal microscopy was used to explore fluorescence patterns. The three (100%) symptomatic subjects and 3 (16%) of the 19 asymptomatic subjects had Flavivirus antigens and viral RNA in the spermatozoa fraction. The percentage of IF Flavivirus-positive spermatozoa cells ranged from 1.9% to 25% in specimens from symptomatic subjects, as compared with 0.8% to 3.8% in specimens from asymptomatic controls. A marked IF-pattern in the cytoplasmic droplets and tail of the spermatozoa was observed. The sperm concentrations (45 × 106/mL vs. 63.5 × 106/mL, p = 0.041) and the total motility percentage (54% vs. 75%, p = 0.009) were significantly lower in specimens from ZIKV-positive than in those of ZIKV-negative. In conclusion, this study demonstrated the presence of Flavivirus antigens and RNA within a time frame of 28 DPSO in sperm cells of symptomatic and asymptomatic subjects during the ZIKV epidemic. These findings have implications for public health, in terms of nonarthropod-born, silent transmission facilitated by sperm cells and potential transmission from asymptomatic males to pregnant women, with consequences to the fetus.
Influenza A Virus Hemagglutinin and Other Pathogen Glycoprotein Interactions with NK Cell Natural Cytotoxicity Receptors NKp46, NKp44, and NKp30
Authors: Luczo Jasmina M. , Ronzulli Sydney L. , Tompkins Stephen M.
Published: January 2021

Natural killer (NK) cells are part of the innate immunity repertoire, and function in the recognition and destruction of tumorigenic and pathogen-infected cells. Engagement of NK cell activating receptors can lead to functional activation of NK cells, resulting in lysis of target cells. NK cell activating receptors specific for non-major histocompatibility complex ligands are NKp46, NKp44, NKp30, NKG2D, and CD16 (also known as FcγRIII). The natural cytotoxicity receptors (NCRs), NKp46, NKp44, and NKp30, have been implicated in functional activation of NK cells following influenza virus infection via binding with influenza virus hemagglutinin (HA). In this review we describe NK cell and influenza A virus biology, and the interactions of influenza A virus HA and other pathogen lectins with NK cell natural cytotoxicity receptors (NCRs). We review concepts which intersect viral immunology, traditional virology and glycobiology to provide insights into the interactions between influenza virus HA and the NCRs. Furthermore, we provide expert opinion on future directions that would provide insights into currently unanswered questions.
A Hypogene Karst Development Pattern Controlled by the Deep-Cycle of Groundwater in the Syncline in Huanjiang, Guangxi, China
Authors: Dong Hongqi , Dan Yong , Liang Jiapeng , Liang Bin , Nie Guoquan , Ji Shaocong
Published: January 2021

Hypogene karst is a special manifestation of karst development in spatial scale. Intensive study of its development mechanism has significant meaning for engineering construction, shale gas and geothermal exploitation. To reveal the developing pattern of hypogene karst in Huanjiang syncline, karst groundwater at different depths in wells HD1-2 and HD1-4 and karst springs was selected as the research object. Through the analysis of geochemistry and stable isotopes of karst groundwater, it was revealed that the circulation pattern of deep karst water came from the common recharge of meteoric water and fossil water hosted in karst caves, runoff of deep faulting belts and discharge of large karst springs, over Huanjiang syncline, which provides good hydrodynamic conditions for hypogene karst development. Meanwhile, the widely developed faulting belts and structural fissures provide primitive dissolution space. Through the above analysis, the paper constructs a hypogene karst development pattern controlled by the deep cycle of groundwater in Huanjiang syncline.
Transport of Cu2+ in Unsaturated Porous Medium with Humic Acid/Iron Oxide Nanoparticle (Fe3O4) Amendment
Authors: Lin Shanshan , Shi Mengdi , Wang Qi , Yang Junlin , Zhang Gubin , Liu Xiangru , Fan Wei
Published: January 2021

Humic acid (HA) and iron oxide (such as Fe3O4) nanoparticles are widely distributed in soil, and their complex embedded in soil might affect the transport and fate of Cu2+ in the vadose zone, while Cu2+ is a serious threat to the underlying groundwater. In this study, we synthesized a composite of Fe3O4 nanoparticles coated with HA (HA@NPs) using as an amendment a packed sand matrix in the transport column experiments. The impacts of HA content and ion strength (IS) on Cu2+ transport in the unsaturated columns were investigated. The results showed that HA exhibited a stronger inhibition effect on Cu2+ transport, and a higher IS enhanced the mobility of Cu2+ in an unsaturated porous medium in the presence of HA@NPs. The recovery ratio (Rr) of Cu2+ breakthrough in the column decreased from 66.56% to 3.94% while the mass concentration ratio CHA/CNPs increased from 0 to 50 in the HA@NPs complex. The Rr increased by 1.64 times while the IS increased from 0 to 100 mM. Batch adsorption experiments, kinetics and isotherm models, and Fourier transform infrared (FTIR) spectra analysis were implemented to elucidate the underlying mechanism. It was found that HA embedded in the sand matrix could bind Cu2+ by forming stable chelate, while the IS-dependent Cu2+ transport could be attributed to the competitive adsorption between Na+ and Cu2+. Our study demonstrates that the physicochemical environment, as well as the presence of iron oxide nanoparticles and natural organic matter, can significantly impact Cu2+ transport in unsaturated porous medium.
Spectral Characterization of Dissolved Organic Matter in Seawater and Sediment Pore Water from the Arctic Fjords (West Svalbard) in Summer
Authors: Chen Meilian , Kim Ji-Hoon , Hong Sungwook , Lee Yun Kyung , Kang Moo Hee , Jin Young Keun , Hur Jin
Published: January 2021

Fjords in the high Arctic, as aquatic critical zones at the interface of land-ocean continuum, are undergoing rapid changes due to glacier retreat and climate warming. Yet, little is known about the biogeochemical processes in the Arctic fjords. We measured the nutrients and the optical properties of dissolved organic matter (DOM) in both seawater and sediment pore water, along with the remote sensing data of the ocean surface, from three West Svalbard fjords. A cross-fjord comparison of fluorescence fingerprints together with downcore trends of salinity, Cl−, and PO43− revealed higher impact of terrestrial inputs (fluorescence index: ~1.2–1.5 in seawaters) and glaciofluvial runoffs (salinity: ~31.4 ± 2.4 psu in pore waters) to the southern fjord of Hornsund as compared to the northern fjords of Isfjorden and Van Mijenfjorden, tallying with heavier annual runoff to the southern fjord of Hornsund. Extremely high levels of protein-like fluorescence (up to ~4.5 RU) were observed at the partially sea ice-covered fjords in summer, in line with near-ubiquity ice-edge blooms observed in the Arctic. The results reflect an ongoing or post-phytoplankton bloom, which is also supported by the higher levels of chlorophyll a fluorescence at the ocean surface, the very high apparent oxygen utilization through the water column, and the nutrient drawdown at the ocean surface. Meanwhile, a characteristic elongated fluorescence fingerprint was observed in the fjords, presumably produced by ice-edge blooms in the Arctic ecosystems. Furthermore, alkalinity and the humic-like peaks showed a general downcore accumulation trend, which implies the production of humic-like DOM via a biological pathway also in the glaciomarine sediments from the Arctic fjords.
The Evaluation of the Accuracy of Interpolation Methods in Crafting Maps of Physical and Hydro-Physical Soil Properties
Authors: Igaz Dušan , Šinka Karol , Varga Peter , Vrbičanová Gréta , Aydın Elena , Tárník Andrej
Published: January 2021

The goal of this study was the spatial processing and showcasing selected soil properties (available water capacity, total organic carbon content and the content of clay fraction <0.001 mm) in the Nitra River Basin (Slovakia) via the usage and the subsequent evaluation of the quality of applied interpolation methods (Spline, inverse distance weighting (IDW), Topo to Raster). The results showed the possibilities of “conversion” of point information obtained by field research as well as research in the laboratory into a spatial expression, thus providing at least relevant estimation of the soil properties even in localities not directly covered by soil sampling. Based on the evaluation and mutual comparison of the accuracy of the used interpolation methods (by using the so-called cross-validation and trust criteria), the most favorable results were achieved by the Spline method in the GRASS GIS environment, and in the ArcGIS environment. When comparing the measured and estimated values of given soil properties at control points, the interpolated values classified as very accurate up to accurate prevailed in the verification dataset. Qualitatively less favorable (but still acceptable) were the results obtained with Topo to Raster (ArcGIS) interpolation method. On the contrary, the Spline method in the ArcGIS environment turned out to be the least accurate. We assume that this is most likely not only a consequence of insufficient density of points (resources), but also an inappropriate implementation of the method into the ArcGIS environment.
Spatio-Temporal Analysis of Surface Water Quality in Mokopane Area, Limpopo, South Africa
Authors: Molekoa Mmasabata , Avtar Ram , Kumar Pankaj , Thu Minh Huynh , Dasgupta Rajarshi , Johnson Brian , Sahu Netrananda , Verma Ram , Yunus Ali
Published: January 2021

Considering the well-documented impacts of land-use change on water resources and the rapid land-use conversions occurring throughout Africa, in this study, we conducted a spatiotemporal analysis of surface water quality and its relation with the land use and land cover (LULC) pattern in Mokopane, Limpopo province of South Africa. Various physico-chemical parameters were analyzed for surface water samples collected from five sampling locations from 2016 to 2020. Time-series analysis of key surface water quality parameters was performed to identify the essential hydrological processes governing water quality. The analyzed water quality data were also used to calculate the heavy metal pollution index (HPI), heavy metal evaluation index (HEI) and weighted water quality index (WQI). Also, the spatial trend of water quality is compared with LULC changes from 2015 to 2020. Results revealed that the concentration of most of the physico-chemical parameters in the water samples was beyond the World Health Organization (WHO) adopted permissible limit, except for a few parameters in some locations. Based on the calculated values of HPI and HEI, water quality samples were categorized as low to moderately polluted water bodies, whereas all water samples fell under the poor category (>100) and beyond based on the calculated WQI. Looking precisely at the water quality’s temporal trend, it is found that most of the sampling shows a deteriorating trend from 2016 to 2019. However, the year 2020 shows a slightly improving trend on water quality, which can be justified by lowering human activities during the lockdown period imposed by COVID-19. Land use has a significant relationship with surface water quality, and it was evident that built-up land had a more significant negative impact on water quality than the other land use classes. Both natural processes (rock weathering) and anthropogenic activities (wastewater discharge, industrial activities etc.) were found to be playing a vital role in water quality evolution. This study suggests that continuous assessment and monitoring of the spatial and temporal variability of water quality in Limpopo is important to control pollution and health safety in the future.
A New Method for Wet-Dry Front Treatment in Outburst Flood Simulation
Authors: Liu Dingzhu , Tang Jinbo , Wang Hao , Cao Yang , Bazai Nazir Ahmed , Chen Huayong , Liu Daochuan
Published: January 2021

When utilizing a finite volume method to predict outburst flood evolution in real geometry, the processing of wet-dry front and dry cells is an important step. In this paper, we propose a new approach to process wet-dry front and dry cells, including four steps: (1) estimating intercell properties; (2) modifying interface elevation; (3) calculating dry cell elevations by averaging intercell elevations; and (4) changing the value of the first term of slope limiter based on geometry in dry cells. The Harten, Lax, and van Leer with the contact wave restored (HLLC) scheme was implemented to calculate the flux. By combining the MUSCL (Monotone Upstream–centred Scheme for Conservation Laws)-Hancock method with the minmod slope limiter, we achieved second-order accuracy in space and time. This approach is able to keep the conservation property (C-property) and the mass conservation of complex bed geometry. The results of numerical tests in this study are consistent with experimental data, which verifies the effectiveness of the new approach. This method could be applied to acquire wetting and drying processes during flood evolution on structured meshes. Furthermore, a new settlement introduces few modification steps, so it could be easily applied to matrix calculations. The new method proposed in this study can facilitate the simulation of flood routing in real terrain.
The Morphological and Functional Organization of Cattails Typha laxmannii Lepech. and Typha australis Schum. and Thonn. under Soil Pollution by Potentially Toxic Elements
Authors: Minkina Tatiana , Fedorenko Grigoriy , Nevidomskaya Dina , Konstantinova Elizaveta , Pol’shina Tatiana , Fedorenko Aleksei , Chaplygin Victor , Mandzhieva Saglara , Dudnikova Tamara , Hassan Tara
Published: January 2021

The aim of this study is to investigate the adaptation of two species of cattail Typha australis Schum. and Thonn. and Typha laxmannii Lepech. based on analysis of the morphological and anatomical features of their vegetative and generative organs to soil pollution with potentially toxic elements (PTE) in the riparian zones of the sea edge of the Don River delta (Southern Russia). Both species of the cattail are able to accumulate high concentrations of Ni, Zn, Cd, Pb and can be used for phytoremediation of polluted territories. The pattern of PTE accumulation in hydrophytes has changed on polluted soils of coastal areas from roots/rhizomes > inflorescences > stems to roots/rhizomes > stems ≥ inflorescences. The comparative morphological and anatomical analysis showed a statistically significant effect of the environmental stress factor by the type of proliferation in T. australis, and species T. laxmannii was visually in a depressed, deformed state with mass manifestations of hypogenesis. These deformations should be considered, on one hand, as adaptive, but on the other, as pathological changes in the structure of the spikes of the cattails. Light-optical and electron microscopic studies have shown that the degree and nature of ultrastructural changes in cattails at the same level of soil pollution are different and most expressed in the assimilation tissue of leaves. However, these changes were destructive for T. australis, but for T. laxmannii, these indicated a high level of adaptation to the prolonged technogenic impact of PTE.
Implications of Experiment Set-Ups for Residential Water End-Use Classification
Authors: Gourmelon Nora , Bayer Siming , Mayle Michael , Bach Guy , Bebber Christian , Munck Christophe , Sosna Christoph , Maier Andreas
Published: January 2021

With an increasing need for secured water supply, a better understanding of the water consumption behavior is beneficial. This can be achieved through end-use classification, i.e., identifying end-uses such as toilets, showers or dishwashers from water consumption data. Previously, both supervised and unsupervised machine learning (ML) techniques are employed, demonstrating accurate classification results on particular datasets. However, a comprehensive comparison of ML techniques on a common dataset is still missing. Hence, in this study, we are aiming at a quantitative evaluation of various ML techniques on a common dataset. For this purpose, a stochastic water consumption simulation tool with high capability to model the real-world water consumption pattern is applied to generate residential data. Subsequently, unsupervised clustering methods, such as dynamic time warping, k-means, DBSCAN, OPTICS and Hough transform, are compared to supervised methods based on SVM. The quantitative results demonstrate that supervised approaches are capable to classify common residential end-uses (toilet, shower, faucet, dishwasher, washing machine, bathtub and mixed water-uses) with accuracies up to 0.99, whereas unsupervised methods fail to detect those consumption categories. In conclusion, clustering techniques alone are not suitable to separate end-use categories fully automatically. Hence, accurate labels are essential for the end-use classification of water events, where crowdsourcing and citizen science approaches pose feasible solutions for this purpose.
Application of Convolution Neural Networks and Hydrological Images for the Estimation of Pollutant Loads in Ungauged Watersheds
Authors: Song Chul Min
Published: January 2021

River monitoring and predicting analysis for establishing pollutant loads management require numerous budgets and human resources. However, it is general that the number of government officials in charge of these tasks is few. Although the government has been commissioning a study related to river management to experts, it has been inevitable to avoid the consumption of a massive budget because the characteristics of pollutant loads present various patterns according to topographic of the watershed, such as topology like South Korea. To address this, previous studies have used conceptual and empirical models and have recently used artificial neural network models. The conceptual model has a shortcoming in which it required massive data and has vexatious that has to enforce the sensitivity and uncertain analysis. The empirical model and artificial neural network (ANN) need lower data than a conceptual model; however, these models have a flaw that could not reflect the topographical characteristic. To this end, this study has used a convolution neural network (CNN), one of the deep learning algorithms, to reflect the topographical characteristic and had estimated the pollutant loads of ungauged watersheds. The estimation results for the biochemical oxygen demand (BOD) and total phosphorus (TP) loads for three ungauged watersheds were all excellent. However, prediction results with low accuracy were obtained when the hydrological images of a watershed with a land cover status different from the ungauged watersheds were used as training data for the CNN model.
Preparation, Performances and Mechanisms of Co@AC Composite for Herbicide Atrazine Removal in Water
Authors: Liu Yongpan , Liu Danxia , He Huijun , Zhang Jinxiao , Liu Jie , Wang Dunqiu , Huang Liangliang , Tu Zhihong
Published: January 2021

In this study, a high-performance adsorbent Co@AC was prepared by loading cobalt ions (Co2+) on activated carbon (AC) via solution impregnation and high-temperature calcination technology, and was used to remove atrazine in water. The preparation factors on the adsorbent properties were studied, and the characteristics were analyzed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier transform infrared spectrometer (FTIR). The results showed that Co@AC possessed the best performance when the factors were 7.0% of Co2+ (w/v), 7.0 h of immersing time, 500 °C of calcination temperature and 4.0 h of calcination time. The adsorption conditions and mechanisms for atrazine removal by Co@AC were also studied scientifically. As the conditions were pH 4.0, reaction time 90 min and temperature 25 °C, Co@AC had the largest adsorption capacity, which was 92.95 mg/g, and the maximum removal rate reached 94.79%. The correlation coefficient of the Freundlich isotherm was better than that of the Langmuir isotherm, and the adsorption process followed the pseudo-second-order kinetic model. Cycle experiments showed that the removal efficiency of atrazine by Co@AC remained above 85% after five repeated experiments, indicating that Co@AC showed a strong stable performance and is a promising material for pesticides removal.