bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Light and Nutrient Starvation on Morphology, Biomass and Lipid Content in Seven Strains of Green Microalgae as a Source of Biodiesel
Authors: Rugnini Lorenza , Rossi Catia , Antonaroli Simonetta , Rakaj Arnold , Bruno Laura
Published: August 2020

The development of clean and renewable energy sources is currently one of the most important challenges facing the world. Although research interests in algae-based energy have been increasing in the last decade, only a small percentage of the bewildering diversity exhibited by microalgae has been investigated for biodiesel production. In this work, seven strains of green microalgae belonging to the genera Scenedesmus, Tetradesmus and Desmodesmus were grown in liquid medium with or without a nitrogen (N) source—at two different irradiances (120 ± 20 and 200 ± 20 μmol photons m−2 s−1)—to evaluate biomass production and FAME (fatty acid methyl esters) content for biodiesel production. The strains of Tetradesmus obliquus and Desmodesmus abundans grown in N-deprived medium showed the highest FAME content (22.0% and 34.6%, respectively); lipid profile characterization highlighted the abundance of saturated FAME (as C16:0 and C18:0) that favors better viscosity (flow properties) and applicability of biodiesel at low temperatures. Light microscopy and confocal laser scanning microscopy observations were employed as a fast method to monitor the vital status of cells and lipid droplet accumulation after Nile red staining in different culture conditions.
pXRF Measurements on Soil Samples for the Exploration of an Antimony Deposit: Example from the Vendean Antimony District (France)
Authors: Lemière Bruno , Melleton Jeremie , Auger Pascal , Derycke Virginie , Gloaguen Eric , Bouat Loïc , Mikšová Dominika , Filzmoser Peter , Middleton Maarit
Published: August 2020

Mineral exploration is increasingly challenging in inhabited areas. To evaluate the potential of soil analysis by pXRF (portable X-ray fluorescence) as a low-footprint exploration technique, we revisited a historic Sb district in an agricultural area and performed shallow-soil sampling (Ah and B horizons) along profiles across known veins to capture the endogenic geochemical anomaly signals. Despite an expected bias between pXRF measurements and laboratory analyses, the former effectively located the Sb veins, especially when using their multi-element capabilities. Composition data processing (CoDa) and horizon-selective sampling significantly improved the method’s efficiency. On-site measurements allow dynamic sampling and mapping, helping with faster, cost-effective sample selection for further laboratory investigations. Based on this case study, where similar geochemical patterns were obtained for both horizons, application of an on-site approach to a humic horizon can increase survey efficiency and decrease impacts.
The Potential Transformation Mechanisms of the Marker Components of Schizonepetae Spica and Its Charred Product
Authors: Liu Xindan , Zhang Ying , Wu Menghua , Ma Zhiguo , Cao Hui
Published: August 2020

Schizonepetae Spica (SS) is commonly used for treating colds, fevers, bloody stool and metrorrhagia in China. To treat colds and fevers, traditional Chinese medicine doctors often use raw SS, while to treat bloody stool and metrorrhagia, they usually use Schizonepetae Spica Carbonisata (SSC; raw SS processed by stir-frying until carbonization). However, there have been limited investigations designed to uncover the mechanism of stir-fry processing. In the present study, a method combining gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) was developed for the comprehensive analysis of the chemical profiles of SS and SSC samples. Principal component analysis of the GC-MS data demonstrated that there were 16 significant differences in volatile compounds between the SS and SSC samples. The simultaneous quantification of six nonvolatile compounds was also established based on HPLC, and remarkable differences were found between the two products. These changes were probably responsible for the various pharmacological effects of SS and SSC as well as the observed hepatotoxicity. Finally, the mechanisms could be rationalized by deducing possible reactions involved in the transformation of these marker components. This work reports a new strategy to reveal the chemical transformation of SS during stir-fry processing.
Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry
Authors: Barrientos Ruth E. , Ahmed Shakeel , Cortés Carmen , Fernández-Galleguillos Carlos , Romero-Parra Javier , Simirgiotis Mario J. , Echeverría Javier
Published: August 2020

Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) is a Chilean endemic plant popularly known as “quiscal” and produces an edible fruit consumed by the local Mapuche communities named as “chupón”. In this study, several metabolites including phenolic acids, organic acids, sugar derivatives, catechins, proanthocyanidins, fatty acids, iridoids, coumarins, benzophenone, flavonoids, and terpenes were identified in G. sphacelata fruits using ultrahigh performance liquid chromatography-photodiode array detection coupled with a Orbitrap mass spectrometry (UHPLC-PDA-Orbitrap-MS) analysis for the first time. The fruits showed moderate antioxidant capacities (i.e., 487.11 ± 26.22 μmol TE/g dry weight) in the stable radical DPPH assay, 169.08 ± 9.81 TE/g dry weight in the ferric reducing power assay, 190.32 ± 6.23 TE/g dry weight in the ABTS assay, and 76.46 ± 3.18% inhibition in the superoxide anion scavenging assay. The cholinesterase inhibitory potential was evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). From the findings, promising results were observed for pulp and seeds. Our findings suggest that G. sphacelata fruits are a rich source of diverse secondary metabolites with antioxidant capacities. In addition, the inhibitory effects against AChE and BChE suggest that natural products or food supplements derived from G. sphacelata fruits are of interest for their neuroprotective potential.
Application of Pineapple Leaves as Adsorbents for Removal of Rose Bengal from Wastewater: Process Optimization Operating Face-Centered Central Composite Design (FCCCD)
Authors: Hassan Siham S. , El-Shafie Ahmed S. , Zaher Nourhan , El-Azazy Marwa
Published: August 2020

Adsorptive removal of rose bengal (RB) from contaminated water samples was approached using pineapple leaves (PAL). Three adsorbents were utilized for that purpose; raw pineapple leaves (RPAL) and the thermally activated bio-waste leaves at 250 and 500 °C. Two measures were executed to evaluate the functionality of exploited biomasses; percentage removal (%R) and adsorption capacity (qe). Face-centered central composite design (FCCCD) was conducted to experiment the influence of variables on the %R. Dose of PAL as adsorbent (AD), concentration of RB (DC), pH and contact time (CT), were the inspected factors. Existence of functional groups and formation of activated carbon was instigated employing Fourier-transform infrared (FT-IR) and Raman spectroscopies. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses were used to explore surface features. Thermal behavior of adsorbents was studied using thermogravimetric analysis (TGA). The surface area and other surface structural properties were established using the Brunauer Emmett-Teller (BET) analysis. An amount of 92.53% of RB could be removed with an adsorption capacity of 58.8 mg/g using a combination of pH 5.00 ± 0.20, RPAL dose of 0.05 mg/50 mL, and 10-ppm RB for 180 min. Equilibrium studies divulge a favorable adsorption that follows the Freundlich isotherm. Pseudo-second-order model explains the observed adsorption kinetics.
Inequality in the Frequency of the Open States Occurrence Depends on Single 2H/1H Replacement in DNA
Authors: Basov Alexander , Drobotenko Mikhail , Svidlov Alexandr , Gerasimenko Eugeny , Malyshko Vadim , Elkina Anna , Baryshev Mikhail , Dzhimak Stepan
Published: August 2020

In the present study, the effect of 2H/1H isotopic exchange in hydrogen bonds between nitrogenous base pairs on occurrence and open states zones dynamics is investigated. These processes are studied using mathematical modeling, taking into account the number of open states between base pairs. The calculations of the probability of occurrence of open states in different parts of the gene were done depending on the localization of the deuterium atom. The mathematical modeling study demonstrated significant inequality (dependent on single 2H/1H replacement in DNA) among three parts of the gene similar in length of the frequency of occurrence of the open states. In this paper, the new convenient approach of the analysis of the abnormal frequency of open states in different parts of the gene encoding interferon alpha 17 was presented, which took into account both rising and decreasing of them that allowed to make a prediction of the functional instability of the specific DNA regions. One advantage of the new algorithm is diminishing the number of both false positive and false negative results in data filtered by this approach compared to the pure fractile methods, such as deciles or quartiles.
Radiochemical Synthesis and Evaluation of Novel Radioconjugates of Neurokinin 1 Receptor Antagonist Aprepitant Dedicated for NK1R-Positive Tumors
Authors: Halik Paweł K. , Lipiński Piotr F. J. , Matalińska Joanna , Koźmiński Przemysław , Misicka Aleksandra , Gniazdowska Ewa
Published: August 2020

Aprepitant, a lipophilic and small molecular representative of neurokinin 1 receptor antagonists, is known for its anti-proliferative activity on numerous cancer cell lines that are sensitive to Substance P mitogen action. In the presented research, we developed two novel structural modifications of aprepitant to create aprepitant conjugates with different radionuclide chelators. All of them were radiolabeled with 68Ga and 177Lu radionuclides and evaluated in terms of their lipophilicity and stability in human serum. Furthermore, fully stable conjugates were examined in molecular modelling with a human neurokinin 1 receptor structure and in a competitive radioligand binding assay using rat brain homogenates in comparison to the aprepitant molecule. This initial research is in the conceptual stage to give potential theranostic-like radiopharmaceutical pairs for the imaging and therapy of neurokinin 1 receptor-overexpressing cancers.
Upcycling of Wastewater via Effective Photocatalytic Hydrogen Production Using MnO2 Nanoparticles—Decorated Activated Carbon Nanoflakes
Authors: Sekar Sankar , Lee Sejoon , Vijayarengan Preethi , Kalirajan Kaliyappan Mohan , Santhakumar Thirumavalavan , Sekar Saravanan , Sadhasivam Sutha
Published: August 2020

In the present work, we demonstrated the upcycling technique of effective wastewater treatment via photocatalytic hydrogen production by using the nanocomposites of manganese oxide-decorated activated carbon (MnO2-AC). The nanocomposites were sonochemically synthesized in pure water by utilizing MnO2 nanoparticles and AC nanoflakes that had been prepared through green routes using the extracts of Brassica oleracea and Azadirachta indica, respectively. MnO2-AC nanocomposites were confirmed to exist in the form of nanopebbles with a high specific surface area of ~109 m2/g. When using the MnO2-AC nanocomposites as a photocatalyst for the wastewater treatment, they exhibited highly efficient hydrogen production activity. Namely, the high hydrogen production rate (395 mL/h) was achieved when splitting the synthetic sulphide effluent (S2− = 0.2 M) via the photocatalytic reaction by using MnO2-AC. The results stand for the excellent energy-conversion capability of the MnO2-AC nanocomposites, particularly, for photocatalytic splitting of hydrogen from sulphide wastewater.
Microwave-Assisted Rapid Synthesis of Reduced Graphene Oxide-Based Gum Tragacanth Hydrogel Nanocomposite for Heavy Metal Ions Adsorption
Authors: Sharma Bhawna , Thakur Sourbh , Trache Djalal , Yazdani Nezhad Hamed , Thakur Vijay Kumar
Published: August 2020

Reduced graphene oxide (RGO) was synthesized in this research via Tour’s method for the use of filler in the hydrogel matrix. The copolymerization of N,N-dimethylacrylamide (DMA) onto the gum tragacanth (GT) was carried out to develop gum tragacanth-cl-N,N-dimethylacrylamide (GT-cl-poly(DMA)) hydrogel using N,N’-methylenebisacrylamide (NMBA) and potassium persulfate (KPS) as cross-linker and initiator correspondingly. The various GT-cl-poly(DMA) hydrogel synthesis parameters were optimized to achieve maximum swelling of GT-cl-poly(DMA) hydrogel. The optimized GT-cl-poly(DMA) hydrogel was then filled with RGO to form reduced graphene oxide incorporated gum tragacanth-cl-N,N-dimethylacrylamide (GT-cl-poly(DMA)/RGO) hydrogel composite. The synthesized samples were used for competent adsorption of Hg2+ and Cr6+ ions. Fourier transform infrared, X-ray powder diffraction, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy were used to characterize the gum tragacanth-cl-N,N-dimethylacrylamide hydrogel and reduced graphene oxide incorporated gum tragacanth-cl-N,N-dimethylacrylamide hydrogel composite. The experiments of adsorption-desorption cycles for Hg2+ and Cr6+ ions were carried out to perform the reusability of gum tragacanth-cl-N,N-dimethylacrylamide hydrogel and reduced graphene oxide incorporated gum tragacanth-cl-N,N-dimethylacrylamide hydrogel composite. From these two samples, reduced graphene oxide incorporated gum tragacanth-cl-N,N-dimethylacrylamide exhibited high adsorption ability. The Hg2+ and Cr6+ ions adsorption by gum tragacanth-cl-N,N-dimethylacrylamide and reduced graphene oxide incorporated gum tragacanth-cl-N,N-dimethylacrylamide were best suited for pseudo-second-order kinetics and Langmuir isotherm. The reported maximum Hg2+ and Cr6+ ions adsorption capacities were 666.6 mg g-1 and 473.9 mg g-1 respectively.
Effects of Multi-Strain Probiotics on Immune Responses and Metabolic Balance in Helicobacter pylori-Infected Mice
Authors: Lin Chun-Che , Huang Wei-Chiao , Su Chiu-Hsian , Lin Wei-De , Wu Wen-Tzu , Yu Bi , Hsu Yuan-Man
Published: August 2020

Chronic inflammation caused by Helicobacter pylori infection increases the risk of developing gastric cancer. Even though the prevalence of H. pylori infection has been decreased in many regions, the development of antibiotic resistance strains has increased the difficulty of eradicating H. pylori. Therefore, exploring alternative approaches to combat H. pylori infection is required. It is well-known that probiotic therapy can improve H. pylori clearance. In this study, H. pylori-infected mice were treated with Lactobacillus fermentum P2 (P2), L. casei L21 (L21), L. rhamnosus JB3 (JB3), or a mixture including the aforementioned three (multi-LAB) for three days. All the lactic acid producing bacteria (LAB) treatments decreased H. pylori loads in the stomach and vacA gene expression, H. pylori specific immunoglobulin (Ig) A, and IgM levels in stomach homogenates, as well as serum levels of interferon-gamma and interleukin-1 beta. The multi-LAB and JB3 treatments further restored the superoxide dismutase and catalase activities suppressed by H. pylori infection. Furthermore, H. pylori infection decreased serum concentrations of 15 kinds of amino acids as well as palmitic acid. The multi-LAB treatment was able to recover the serum levels of alanine, arginine, aspartate, glycine, and tryptophan, which are all important in modulating immune functions. In addition, butyric acid, valeric acid, palmitic acid, palmitoleic acid, stearic acid, and oleic acid levels were increased. In this study, multi-LAB revealed its ability to adjust the composition of metabolites to improve health. To date, the mechanisms underlying how LAB strains crosstalk with the host are not fully understood. Identifying the mechanisms which are regulated by LABs will facilitate the development of effective therapies for infection in the future.
Molecular Characterisation of a Rare Reassortant Porcine-Like G5P[6] Rotavirus Strain Detected in an Unvaccinated Child in Kasama, Zambia
Authors: Maringa Wairimu M. , Mwangi Peter N. , Simwaka Julia , Mpabalwani Evans M. , Mwenda Jason M. , Peenze Ina , Esona Mathew D. , Mphahlele M. Jeffrey , Seheri Mapaseka L. , Nyaga Martin M.
Published: August 2020

A human-porcine reassortant strain, RVA/Human-wt/ZMB/UFS-NGS-MRC-DPRU4723/2014/G5P[6], was identified in a sample collected in 2014 from an unvaccinated 12 month old male hospitalised for gastroenteritis in Zambia. We sequenced and characterised the complete genome of this strain which presented the constellation: G5-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The genotype A8 is often observed in porcine strains. Phylogenetic analyses showed that VP6, VP7, NSP2, NSP4, and NSP5 genes were closely related to cognate gene sequences of porcine strains (e.g., RVA/Pig-wt/CHN/DZ-2/2013/G5P[X] for VP7) from the NCBI database, while VP1, VP3, VP4, and NSP3 were closely related to porcine-like human strains (e.g., RVA/Human-wt/CHN/E931/2008/G4P[6] for VP1, and VP3). On the other hand, the origin of the VP2 was not clear from our analyses, as it was not only close to both porcine (e.g., RVA/Pig-tc/CHN/SWU-1C/2018/G9P[13]) and porcine-like human strains (e.g., RVA/Human-wt/LKA/R1207/2009/G4P[6]) but also to three human strains (e.g., RVA/Human-wt/USA/1476/1974/G1P[8]). The VP7 gene was located in lineage II that comprised only porcine strains, which suggests the occurrence of independent porcine-to-human reassortment events. The study strain may have collectively been derived through interspecies transmission, or through reassortment event(s) involving strains of porcine and porcine-like human origin. The results of this study underline the importance of whole-genome characterisation of rotavirus strains and provide insights into interspecies transmissions from porcine to humans.
Cystatin C and α-1-Microglobulin Predict Severe Acute Kidney Injury in Patients with Hemorrhagic Fever with Renal Syndrome
Authors: Hansson Magnus , Gustafsson Rasmus , Jacquet Chloé , Chebaane Nedia , Satchell Simon , Thunberg Therese , Ahlm Clas , Fors Connolly Anne-Marie
Published: August 2020

Puumala orthohantavirus causes hemorrhagic fever with renal syndrome (HFRS) characterized by acute kidney injury (AKI), an abrupt decrease in renal function. Creatinine is routinely used to detect and quantify AKI; however, early AKI may not be reflected in increased creatinine levels. Therefore, kidney injury markers that can predict AKI are needed. The potential of the kidney injury markers urea, cystatin C, α1-microglobulin (A1M) and neutrophil gelatinase-associated lipocalin (NGAL) to detect early AKI during HFRS was studied by quantifying the levels of these markers in consecutively obtained plasma (P) and urine samples (U) for 44 HFRS patients. P-cystatin C and U-A1M levels were significantly increased during early HFRS compared to follow-up. In a receiver operating characteristic (ROC) curve analysis, P-cystatin C, U-A1M and P-urea predicted severe AKI with area under the curve 0.72, 0.73 and 0.71, respectively, whereas the traditional kidney injury biomarkers creatinine and U-albumin did not predict AKI. Nearly half of the HFRS patients (41%) fulfilled the criteria for shrunken pore syndrome, which was associated with the level of inflammation as measured by P-CRP. P-cystatin C and U-A1M are more sensitive and earlier markers compared to creatinine in predicting kidney injury during HFRS.
Photochemical Efficiency and Oxidative Metabolism of Tree Species during Acclimation to High and Low Irradiance
Authors: da Rocha Nina Junior Adamir , Furtunato Maia Jair Max , Vitor Martins Samuel Cordeiro , Gonçalves José Francisco de Carvalho
Published: August 2020

The balance between efficiency of absorption and use of light energy is fundamental for plant metabolism and to avoid photoinhibition. Here, we investigated the effects of light environments on the photosynthetic apparatus of tropical tree species of three successional groups (pioneer, mid-, and late successional) subjected to different light conditions: full sunlight (FS), moderate shade (MS), and deep shade (DS). Twenty-nine ecophysiological parameters were correlated with each other. The pioneer species exhibited better photochemical performance and a more efficient antioxidant enzymatic system in comparison with the other successional groups. Plants in FS showed higher intensity of lipid peroxidation, with superoxide dismutase having a prominent role in the antioxidant system. At lower irradiance the enzymatic activity was reduced, and the photochemical efficiency was the preferred way to reduce oxidative damages. P was highly related to photochemical yield, and the N modulation amplified the light harvesting complex in DS to the detriment of the antioxidant system. Despite evidence of cell damage, most species exhibited the ability to adjust to high irradiance. Contrary to expectations, Hymenea courbaril (late-successional) exhibited higher plasticity to fluorescence, nutritional, and antioxidant parameters. Only Carapa guianensis (late-successional) displayed photoinhibitory damage in FS, and Ochroma pyramidale (pioneer) did not survive in DS, suggesting that acclimation to shade is more challenging than to high irradiance.
Nutrient Diagnosis of Eucalyptus at the Factor-Specific Level Using Machine Learning and Compositional Methods
Authors: Vahl de Paula Betania , Squizani Arruda Wagner , Etienne Parent Léon , Frank de Araujo Elias , Brunetto Gustavo
Published: August 2020

Brazil is home to 30% of the world’s Eucalyptus trees. The seedlings are fertilized at plantation to support biomass production until canopy closure. Thereafter, fertilization is guided by state standards that may not apply at the local scale where myriads of growth factors interact. Our objective was to customize the nutrient diagnosis of young Eucalyptus trees down to factor-specific levels. We collected 1861 observations across eight clones, 48 soil types, and 148 locations in southern Brazil. Cutoff diameter between low- and high-yielding specimens at breast height was set at 4.3 cm. The random forest classification model returned a relatively uninformative area under the curve (AUC) of 0.63 using tissue compositions only, and an informative AUC of 0.78 after adding local features. Compared to nutrient levels from quartile compatibility intervals of nutritionally balanced specimens at high-yield level, state guidelines appeared to be too high for Mg, B, Mn, and Fe and too low for Cu and Zn. Moreover, diagnosis using concentration ranges collapsed in the multivariate Euclidean hyper-space by denying nutrient interactions. Factor-specific diagnosis detected nutrient imbalance by computing the Euclidean distance between centered log-ratio transformed compositions of defective and successful neighbors at a local scale. Downscaling regional nutrient standards may thus fail to account for factor interactions at a local scale. Documenting factors at a local scale requires large datasets through close collaboration between stakeholders.
Comparative Microbiome Study of Mummified Peach Fruits by Metagenomics and Metatranscriptomics
Authors: Jo Yeonhwa , Back Chang-Gi , Choi Hoseong , Cho Won Kyong
Published: August 2020

The dried peach fruits clinging to peach trees or lying on the ground nearby are known as mummified peach fruits. Here, we examined the microbiome communities of three different mummified peach fruits from the nectarine cultivar “Hahong” by DNA- and RNA-sequencing. We found the dominance of Monilinia fructigena followed by Sclerotinia borealis, S. sclerotiorum, and Botrytis cinerea in the mummified peach fruits. Moreover, we found a high number of Proteobacteria, including Frateuria aurantia, Neoasaia chiangmaiensis, Robbsia andropogonis, and Ewingella Americana. Furthermore, we identified several viruses and viroids. Bacteriophages were identified by DNA- and RNA-sequencing, while viruses and viroids with RNA genomes were identified by only RNA-sequencing. Moreover, we identified a novel mycovirus referred to as Monilinia umbra-like virus 1 (MULV1) from M. fructigena. Our results revealed the co-inhabitance of fungi and bacteria in the mummified peach fruits, although dominant microorganisms were present. RNA-sequencing revealed that several fungal and bacterial genes were actively transcribed. Comparative analyses suggested that RNA-sequencing provides more detailed information on microbial communities; however, combining DNA- and RNA-sequencing results increased the diversity of microorganisms, suggesting the importance of databases and analysis tools for microbiome studies. Taken together, our study provides a comprehensive overview of microbial communities in mummified peach fruits by DNA shotgun sequencing and RNA-sequencing.
10 kV SiC MOSFET Evaluation for Dielectric Barrier Discharge Transformerless Power Supply
Authors: Diop Mame Andallah , Belinger Antoine , Piquet Hubert
Published: August 2020

At low pressure, cold plasmas are used for a wide range of applications such as coating, flow control, or microelectronics. Currently, this industry requires expensive vacuum systems which consume energy and time, and therefore it is very appealing to develop similar processes at atmospheric pressure. Under this condition, dielectric barrier discharge (DBD) is one of the best ways to obtain a cold plasma. The dielectric barriers naturally limit the current, and then the plasma temperature. Unfortunately, at atmospheric pressure the discharge ignition between the electrodes requires high voltage, which is generally obtained through a step-up transformer. The parasitic elements of this device exclude a smart control for the discharge. In order to overcome this default, we analyze the performance of a transformerless power supply developed with a recently released single-chip high-voltage semiconductor. The circuit uses only two high-voltage switches synthesized by means of the 10 kV SiC MOSFET (Metal Oxide Semiconductor Field Effect Transistor). The design and implementation of the electric converter are presented and validated with experiments carried out on UV excimer DBD lamps. Then, the performances of the 10 kV SiC switches are analyzed and the relevance of this device for DBD applications is discussed.
Synthesis and Properties of Moisture-Cured Reactive Polyurethane Containing Castor Oil and Oxime Compounds
Authors: Wu Zheng-Ying
Published: August 2020

Reactive polyurethane hot-melt resin (moisture-cured reactive polyurethane, PUR) could successfully be prepared from poly(tetramethylene ether) glycol (PTMG), castor oil and dimethylglyoxime (DMG) by one or two-stage synthesis. Fourier-transform infrared spectroscopy (FTIR) analysis showed that the synthesis resins belonged to NCO-capped castor oil-based polyurethane. The thermal behaviors of the cured PUR were analyzed by differential scanning calorimeter (DSC) and dynamic mechanical analyzer (DMA) instruments. The results showed that the cured resin provided remeltable properties under the dosages of 3 wt% DMG. Furthermore, the phenomenon could be proved by FTIR analysis according to the characteristic absorption peak of NCO groups after the cured resin was heated. Comparing different syntheses, the resin prepared by one-stage synthesis showed random distribution of DMG with PUR structure and that prepared by two-stage synthesis had distribution of DMG with branching structure in the prepolymer. The former obtained lower remeltable temperatures from 90 to 130 °C than the latter temperatures, which had temperatures above 125 °C. The tensile test showed that all of the PUR films exhibited typical tough behavior. Thus, the cured resin with DMG dosages of 3 wt% provided remeltable and mechanical properties at the same time. Overall, the crosslinking density and numbers of dynamic bonds should be kept in balance for preparation of remeltable PUR.
Production of Biodegradable Palm Oil-Based Polyurethane as Potential Biomaterial for Biomedical Applications
Authors: Yeoh Fang Hoong , Lee Choy Sin , Kang Yew Beng , Wong Shew Fung , Cheng Sit Foon , Ng Wei Seng
Published: August 2020

Being biodegradable and biocompatible are crucial characteristics for biomaterial used for medical and biomedical applications. Vegetable oil-based polyols are known to contribute both the biodegradability and biocompatibility of polyurethanes; however, petrochemical-based polyols were often incorporated to improve the thermal and mechanical properties of polyurethane. In this work, palm oil-based polyester polyol (PPP) derived from epoxidized palm olein and glutaric acid was reacted with isophorone diisocyanate to produce an aliphatic polyurethane, without the incorporation of any commercial petrochemical-based polyol. The effects of water content and isocyanate index were investigated. The polyurethanes produced consisted of > 90% porosity with interconnected micropores and macropores (37–1700 µm) and PU 1.0 possessed tensile strength and compression stress of 111 kPa and 64 kPa. The polyurethanes with comparable thermal stability, yet susceptible to enzymatic degradation with 7–59% of mass loss after 4 weeks of treatment. The polyurethanes demonstrated superior water uptake (up to 450%) and did not induce significant changes in pH of the medium. The chemical changes of the polyurethanes after enzymatic degradation were evaluated by FTIR and TGA analyses. The polyurethanes showed cell viability of 53.43% and 80.37% after 1 and 10 day(s) of cytotoxicity test; and cell adhesion and proliferation in cell adhesion test. The polyurethanes produced demonstrated its potential as biomaterial for soft tissue engineering applications.
Influence of the Architecture of Soft Polymer-Functionalized Polymer Nanoparticles on Their Dynamics in Suspension
Authors: Kim Young-Gon , Wichaita Waraporn , Thérien-Aubin Héloïse
Published: August 2020

The behavior of nanogels in suspension can be dramatically affected by the grafting of a canopy of end-tethered polymer chains. The architecture of the interfacial layer, defined by the grafting density and length of the polymer chains, is a crucial parameter in defining the conformation and influencing the dynamics of the grafted chains. However, the influence of this architecture when the core substrate is itself soft and mobile is complex; the dynamics of the core influences the dynamics of the tethered chains, and, conversely, the dynamics of the tethered chains can influence the dynamics of the core. Here, poly(styrene) (PS) particles were functionalized with poly(methyl acrylate) (PMA) chains and swollen in a common solvent. NMR relaxation reveals that the confinement influences the mobility of the grafted chain more prominently for densely grafted short chains. The correlation time associated with the relaxation of the PMA increased by more than 20% when the grafting density increased for short chains, but for less than 10% for long chains. This phenomenon is likely due to the steric hindrance created by the close proximity to the rigid core and of the neighboring chains. More interestingly, a thick layer of a densely grafted PMA canopy efficiently increases the local mobility of the PS cores, with a reduction of the correlation time of more than 30%. These results suggest an interplay between the dynamics of the core and the dynamics of the canopy.
Improvement of PLLA Ductility by Blending with PVDF: Localization of Compatibilizers at Interface and Its Glycidyl Methacrylate Content Dependency
Authors: Zhang Yan , Gu Xiaoying , Ni Chunjun , Li Fei , Li Yongjin , You Jichun
Published: August 2020

In this work, the localization of reactive compatibilizer (RC, containing poly(methyl methacrylate) (PMMA) backbone with randomly distributed glycidyl methacrylate (GMA) on it) at the polyvinylidene fluoride/poly(l-lactic acid) (PVDF/PLLA) interface has been manipulated by means of GMA contents. At the very beginning of mixing, RC tends to stay in the PVDF phase due to the miscibility between PVDF and PMMA. Upon further shearing, more and more PLLA chains have been grafted on PMMA backbone, producing PLLA–g–PMMA copolymer. The balanced stress on two sides accounts for the localization of compatibilizers at the PVDF/PLLA interface. Finally, the stress of the PLLA side has been enhanced remarkably due to the higher graft density of PLLA, resulting in the enrichment of the copolymer in the PLLA matrix. The migration of RC from the PVDF phase to the immiscible interface and PLLA matrix can be accelerated by employing RC with higher GMA content. Furthermore, the compatibilizer localization produces a significant influence on the morphology and ductility of the PVDF/PLLA blend. Only when the compatibilizers precisely localize at the interface, the blend exhibits the smallest domain and highest elongation at break. Our results are of great significance for not only the fabrication of PLLA with high ductility, but also the precise localization of compatibilizers at the interface of the immiscible blend.
Natural Formulations Provide Antioxidant Complement to Hyaluronic Acid-Based Topical Applications Used in Wound Healing
Authors: Makvandi Pooyan , Caccavale Caterina , Della Sala Francesca , Zeppetelli Stefania , Veneziano Rosanna , Borzacchiello Assunta
Published: August 2020

Hyaluronic acid (HA) promotes wound healing, and, accordingly, formulations based on HA have been widely used in regenerative medicine. In addition, naturally derived compounds, e.g., plant-based extracts and vitamin E, have exhibited antioxidant activity. In this study, a formulation containing hyaluronic acid, vitamin E, raspberry extract, and green tea was developed for potential topical applications, targeting wound healing. Rheological analysis was performed along with antioxidant and biological studies. The rheological characterization showed that the HA-based formulation is a thixotropic platform and possesses higher mechanical properties than the control formulation. To evaluate the wound healing potential of the formulation, an in vitro “wound healing” assay was carried out using human derived fibroblasts (HDF) with a cell-free gap on the tissue culture dish. The formulation showed better wound healing ability than the control formulation.
Hydrolytic Degradation of Porous Crosslinked Poly(ε-Caprolactone) Synthesized by High Internal Phase Emulsion Templating
Authors: Erdal Nejla Benyahia , Lando Gabriela Albara , Yadav Anilkumar , Srivastava Rajiv K. , Hakkarainen Minna
Published: August 2020

Porous poly(ε-caprolactone) (PCL) scaffolds were fabricated using the high internal polymerization emulsion (HIPE) technique. Bis(ε-caprolactone-4-yl) (BCY) was utilized as crosslinker. The crosslinking density and the volume fraction of the dispersed phase were varied in order to study the potential effect of these parameters on the hydrolytic degradation at 37 °C and 60 °C. After different hydrolysis times the remaining solid samples were analyzed by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), while the degradation products in the aqueous aging solutions were analyzed by laser desorption ionization-mass spectrometry (LDI-MS). The effect of temperature on the degradation process and release of degradation products was, as expected, significant. The temperature effect was also shown by FTIR analysis that displayed a pronounced increase in the intensity of the hydroxyl-group absorption band after 70 days of hydrolysis at 60 °C indicating significant cleavage of the polymer chains. LDI-MS analysis proved the release of oligomers ranging from dimers to hexamers. The product patterns were similar, but the relative m/z signal intensities increased with increasing time, temperature and crosslinking density, indicating larger amounts of released products. The latter is probably due to the decreasing degree of crystallinity as a function of amount of crosslinker. The porous structure and morphology of the scaffolds were lost during the aging. The higher the crosslinking density, the longer the scaffolds retained their original porous structure and morphology.
Long-Term Formaldehyde Emission Potential from UF- and NAF-Bonded Particleboards
Authors: Frihart Charles R. , Chaffee Timothy L. , Wescott James M.
Published: August 2020

As a result of the dominance of urea formaldehyde (UF)-bonded particleboard, it seemed worthwhile to examine formaldehyde emissions years after production. A California Air Resources Board (CARB) phase II-compliant commercial particleboard produced with a UF resin adhesive was compared to a no-added formaldehyde (NAF)-particleboard produced with Soyad™ adhesive resin for formaldehyde emissions during exposure to elevated humidity and temperature conditions after being in a room at 21 ± 1.9 °C, 50 ± 3.3% relative humidity for 3.5 years. A modified version of EN 717-3 was used to collect formaldehyde emissions under typical along with higher temperature and humidity conditions. The formaldehyde emissions from the commercial particleboard panel bonded with a UF adhesive even after the 3.5 years of exposure greatly increased only during exposure of the panels to elevated heat and humidity compared to typical testing conditions. The amounts were the same as those with the previous shorter-term study. In contrast, formaldehyde emissions from the NAF-bonded particleboard were not as susceptible (in absolute terms) to increases in temperature and relative humidity conditions.
Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis Essential Oils as Antifungal Activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum
Authors: Elgat Wael A.A. Abo , Kordy Ahmed M. , Böhm Martin , Černý Robert , Abdel-Megeed Ahmed , Salem Mohamed Z.M.
Published: August 2020

Several molds are able to colonize wood and many building products or solid wood causing losses for their valuable uses. Essential oils (EOs) from aromatic plants can be used as an ecofriendly biofungicide against the growth of several molds. EOs from Eucalyptus camaldulensis, Citrus aurantium, and C. sinensis have a broad-spectrum antimicrobial activity. EOs from of E. camaldulensis air-dried aerial parts, C. aurantium leaf and C. sinensis peel, and their combinations (1:1 v/v) were evaluated for their antifungal activity against the growth of four common mold fungi (Aspergillus flavus, A. niger, A. terreus, and Fusarium culmorum). The chemical compositions of the EOs were analyzed with GC/MS. The main compounds in EO from E. camaldulensis were spathulenol (20.84%), eucalyptol (12.01%), and sabinene (9.73%); in C. aurantium were linalyl acetate (42.29%), and linalool (29.76%); and in C. sinensis were D-limonene (73.4%) and γ-terpinene (22.6%). At 50 µL/mL, C. sinensis EO showed the highest fungal mycilial growth inhibition (FMGI) percentage (86.66%) against A. flavus. C. sinensis, E. camaldulensis, and E. camaldulensis/C. sinensis showed FMGI values of 96%, 91.66%, and 75.66% respectively, against A. niger. EOs from C. aurantium and C. sinensis showed potent activity against A. terreus (100% FMGI), while C. aurantium/E. camaldulensis and E. camaldulensis/C. sinensis showed FMGI values of 74.33% and 70.66%, respectively. Potent activity against F. culmorum with 100% was observed as the application of E. camaldulensis and C. sinensis EOs at 50 µL/mL, while E. camaldulensis/C. sinensis (50 µL/mL) showed FMGI value of 65.66%. The results suggest using the EOs and their combinations from E.camaldulensis, C. aurantium, and C. sinensis as a biofungicide against molds. The potent properties of EOs offer the possibility of using them as eco-friendly, safe, and cost-effective antimicrobials for molds that could cause discoloration of the wood packaging or food spoilage.
The Digital Integrated Workflow in the Aesthetic Management of the Smile: A Case Report
Authors: Scattarelli Paolo , Smaniotto Paolo , Leuci Serena , Cervino Gabriele , Gisotti Mario
Published: August 2020

The aesthetic treatment for anterior teeth requires a series of clinical and technical evaluations to obtain a predictable result, following a well-defined operating sequence. Today, the clinical–technical team can use different digital tools in the different steps of the workflow. A preventive assessment, the knowledge of limits, and the possibilities of surgical and prosthetic procedures allow to use these devices. Sharing goals of the treatment with the patient according to their expectations and needs is the key point of the treatment plan. Setting a defined treatment plan avoids invasive procedures. In this clinical case report, a 27-year-old patient affected by Class II div 2 malocclusion with deep bite was treated with a full digital workflow. Previewing the aesthetics was through dedicated software, which shows operators and patients the objectives of the therapy and guides the dental technician in the first phase of the work. The use of digital flows in the prosthetic phases reduces the working time. In this case, it shows the impact of a digital workflow on peri-prosthetic therapy for the aesthetic rehabilitation of the upper central incisors in a young adult.
Neurodevelopmental Disorders Induced by Maternal Immune Activation: Toward a Prevention Strategy in the Era of the COVID-19 Pandemic
Authors: Sakurada Kazuhiro , Noda Yoshihiro
Published: August 2020

As of summer 2020, the COVID-19 pandemic is having a major impact on our daily lives on a global scale, forcing us to change to the new normal. However, the effects are not only detrimental to our present socioeconomic conditions but also have the risk of having negative biological effects on our descendants. Of concern is the effect of maternal immune activation following maternal infection with COVID-19 on the fetus’ cerebral nervous system. While we are currently occupied with countering the imminent threats in front of us, we also need to take steps from a public health perspective to reduce the impact of maternal infection on the fetus, especially the risk of neurodevelopmental disorders. However, such a risk can be prevented and managed through the digital transformation of the nation’s health data and the strategic application of sophisticated data science approaches to those big data.
Community and Naming: Lived Narratives of Early African American Women’s Spirituality
Authors: West Elizabeth J.
Published: August 2020

Through the story of Francis Sistrunk, nineteenth century enslaved and later freedwoman in east central Mississippi, this essay illustrates that, despite few surviving written narratives of early black women’s spirituality, their experiences can emerge from the silences. Much like paleontologists who recreate narratives of the past through fossils, in the present world of literary studies, we have the advantage of an expanse of resources that, when pieced together, can convey voices from the past to the present. This includes resources such as extant oral and written communal and family narratives, generational ideals and practices, digitized records from official and personal documents, and the recent emergence of DNA technology that provides its own narratives. From the earliest arrivals to the Americas, African diasporic populations maintained an understanding of community and spirit as an integrated oneness empowered through the word, particularly in the word-act of naming. Francis’ story reveals that this spiritual ethos was a generative source, not only for survival, but for some black women it was a mechanism for inscribing their presence, their narratives, and their legacies for future generations. Francis Sistrunk’s story re-emerges through the mining of sources such as these, and reveals that enslaved black women reached for and seized power where they found it to preserve the record of their existence and humanity and to record the story of their enslavers’ injustices.
Exchange Rate, Gold Price, and Stock Market Nexus: A Quantile Regression Approach
Authors: Ali Rizwan , Mangla Inayat Ullah , Rehman Ramiz Ur , Xue Wuzhao , Naseem Muhammad Akram , Ahmad Muhammad Ishfaq
Published: August 2020

In this study, we examine an empirical relationship between stock market volatility with the exchange rate and gold prices of an emerging market, “Pakistan”, employing daily and monthly data (PSX-100 Index) covering from 2001: Q3 to 2018: Q2. The study explains the average stock returns by applying MGARCH. Further, it investigates that the volatility in the exchange rate (Rs/US $) and gold prices remain equally strong in bearish and bullish conditions of the stock market by using a quantile regression approach (2001–2018). Additionally, the sample period is divided into two split samples that cover (2001–2007) and (2008–2018) respectively, based on global financial crises and applied similar analysis. The overall results show the negative impact of the exchange rate and gold price volatility on the stock market performance daily (monthly), supporting the argument that the stock market considers the exchange rate and gold price fluctuations as an adverse indicator and reacts negatively.
Effective Training of Deep Convolutional Neural Networks for Hyperspectral Image Classification through Artificial Labeling
Authors: Masarczyk Wojciech , Głomb Przemysław , Grabowski Bartosz , Ostaszewski Mateusz
Published: August 2020

Hyperspectral imaging is a rich source of data, allowing for a multitude of effective applications. However, such imaging remains challenging because of large data dimension and, typically, a small pool of available training examples. While deep learning approaches have been shown to be successful in providing effective classification solutions, especially for high dimensional problems, unfortunately they work best with a lot of labelled examples available. The transfer learning approach can be used to alleviate the second requirement for a particular dataset: first the network is pre-trained on some dataset with large amount of training labels available, then the actual dataset is used to fine-tune the network. This strategy is not straightforward to apply with hyperspectral images, as it is often the case that only one particular image of some type or characteristic is available. In this paper, we propose and investigate a simple and effective strategy of transfer learning that uses unsupervised pre-training step without label information. This approach can be applied to many of the hyperspectral classification problems. The performed experiments show that it is very effective at improving the classification accuracy without being restricted to a particular image type or neural network architecture. The experiments were carried out on several deep neural network architectures and various sizes of labeled training sets. The greatest improvement in overall accuracy on the Indian Pines and Pavia University datasets is over 21 and 13 percentage points, respectively. An additional advantage of the proposed approach is the unsupervised nature of the pre-training step, which can be done immediately after image acquisition, without the need of the potentially costly expert’s time.