bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical, biochemical and molecular analysis in a cohort of individuals with gyrate atrophy
Authors: Palmer Eleanor , Stepien Karolina M. , Campbell Christopher , Barton Stephanie , Iosifidis Christos , Ghosh Arunabha , Broomfield Alexander , Woodall Alison , Wilcox Gisela , Sergouniotis Panagiotis I. , Black Graeme C.
Published: September 2023

Background

Gyrate atrophy of the choroid and retina is a rare autosomal recessive metabolic disorder caused by biallelic variants in the OAT gene, encoding the enzyme ornithine δ-aminotransferase. Impaired enzymatic activity leads to systemic hyperornithinaemia, which in turn underlies progressive chorioretinal degeneration. In this study, we describe the clinical and molecular findings in a cohort of individuals with gyrate atrophy.

Methods

Study participants were recruited through a tertiary UK clinical ophthalmic genetic service. All cases had a biochemical and molecular diagnosis of gyrate atrophy. Retrospective phenotypic and biochemical data were collected using electronic healthcare records.

Results

18 affected individuals from 12 families (8 male, 10 female) met the study inclusion criteria. The median age at diagnosis was 8 years (range 10 months – 33 years) and all cases had hyperornithinaemia (median: 800 micromoles/L; range: 458–1244 micromoles/L). Common features at presentation included high myopia (10/18) and nyctalopia (5/18). Ophthalmic findings were present in all study participants who were above the age of 6 years. One third of patients had co-existing macular oedema and two thirds developed pre-senile cataracts. Compliance with dietary modifications was suboptimal in most cases. A subset of participants had extraocular features including a trend towards reduced fat-free mass and developmental delay.

Conclusions

Our findings highlight the importance of multidisciplinary care in families with gyrate atrophy. Secondary ophthalmic complications such as macular oedema and cataract formation are common. Management of affected individuals remains challenging due to the highly restrictive nature of the recommended diet and the limited evidence-base for current strategies.
Parental perspectives of episodic irritability in an ultra-rare genetic disorder associated with NACC1
Authors: Schoch Kelly , McConkie-Rosell Allyn , Walley Nicole , Bhambhani Vikas , Feyma Timothy ,   Undiagnosed Diseases Network, Pizoli Carolyn E. , Smith Edward C. , Tan Queenie K.-G. , Shashi Vandana
Published: September 2023

Background

A recurrent de novo variant (c.892C>T) in NACC1 causes a neurodevelopmental disorder with epilepsy, cataracts, feeding difficulties, and delayed brain myelination (NECFM). An unusual and consistently reported feature is episodic extreme irritability and inconsolability. We now characterize these episodes, their impact on the family, and ascertain treatments that may be effective. Parents of 14 affected individuals provided narratives describing the irritability episodes, including triggers, behavioral and physiological changes, and treatments. Simultaneously, parents of 15 children completed the Non-communicating Children’s Pain Checklist-Revised (NCCPC-R), a measure to assess pain in non-verbal children.

Results

The episodes of extreme irritability include a prodromal, peak, and resolving phase, with normal periods in between. The children were rated to have extreme pain-related behaviors on the NCCPC-R scale, although it is unknown whether the physiologic changes described by parents are caused by pain. Attempted treatments included various classes of medications, with psychotropic and sedative medications being most effective (7/15). Nearly all families (13/14) describe how the episodes have a profound impact on their lives.

Conclusions

NECFM caused by the recurrent variant c.892C>T is associated with a universal feature of incapacitating episodic irritability of unclear etiology. Further understanding of the pathophysiology can lead to more effective therapeutic strategies.
Prioritizing educational initiatives on emerging technologies for Italian pediatricians: bibliometric review and a survey
Authors: Tozzi Alberto Eugenio , Gesualdo Francesco , Pandolfi Elisabetta , Ferro Diana , Cinelli Giulia , Bozzola Elena , Aversa Tommaso , Di Mauro Antonio , Mameli Chiara , Croci Ileana
Published: September 2023

Background

Emerging technologies have demonstrated outstanding potential in improving healthcare, yet their full integration remains a challenge for all medical specialties, including pediatrics. To support the swift implementation of technologies, we identified the current trends through a bibliometric review, and we conducted a survey on Italian pediatricians to gauge educational needs and willingness to integrate technologies into clinical practice.

Methods

A working group of pediatricians representing various backgrounds designed and coordinated the study. To identify relevant topics for educational strategy development, we focused on virtual reality, telehealth, natural language processing, smartphone applications, robotics, genomics, and artificial intelligence. A bibliometric analysis limited to 2018–2023 was performed to identify trends and emerging applications within each topic. Based on the results, a questionnaire was developed and made available online to all Italian pediatricians. The results were analyzed through descriptive analysis and a multivariable logistic regression to explore associations between technology adoption and sociodemographic characteristics.

Results

A total of 3,253 publications were found, with Telehealth and Telemedicine having the highest number of publications and Natural Language Processing the lowest. The number of respondents to the online questionnaire was 1,540, predominantly medical doctors with over 20 years of experience working as family pediatricians. Telehealth had the highest level of knowledge (95.2%), followed by smartphone applications (89.1%) and genomics (63.2%). The greatest potential for increased use through education programs was projected for natural language processing (+ 43.1%), artificial intelligence (+ 39.6%), and virtual and mixed reality (+ 38.1%). Female respondents and older individuals were less likely to use emerging technologies. Hospital pediatricians and residents were more likely to use AI.

Conclusions

We developed a replicable strategy to identify emerging themes in medical technologies relevant to pediatrics and assess the educational needs of pediatricians. A significant gap still exists between current and potential usage of emerging technologies among Italian pediatricians although they showed a positive attitude towards implementing these technologies following specific education programs. The study highlights the need for comprehensive education programs on emerging technologies in pediatrics and recommends addressing gender and age disparities in technology adoption.
Perilipin 5 deficiency aggravates cardiac hypertrophy by stimulating lactate production in leptin-deficient mice
Authors: Jian Lele , Gao Xing , Wang Chao , Sun Xiao , Xu Yuqiao , Han Ruili , Wang Yuying , Xu Shenhui , Ding Lan , Zhou Jingjun , Gu Yu , Zhao Yuanlin , Yang Ying , Yuan Yuan , Ye Jing , Zhang Lijun
Published: September 2023

Background

Perilipin 5 (Plin5) is well known to maintain the stability of intracellular lipid droplets (LDs) and regulate fatty acid metabolism in oxidative tissues. It is highly expressed in the heart, but its roles have yet to be fully elucidated.

Methods

Plin5-deficient mice and Plin5/leptin-double-knockout mice were produced, and their histological structures and myocardial functions were observed. Critical proteins related to fatty acid and glucose metabolism were measured in heart tissues, neonatal mouse cardiomyocytes and Plin5-overexpressing H9C2 cells. 2-NBDG was employed to detect glucose uptake. The mitochondria and lipid contents were observed by MitoTracker and BODIPY 493/503 staining in neonatal mouse cardiomyocytes.

Results

Plin5 deficiency impaired glucose utilization and caused insulin resistance in mouse cardiomyocytes, particularly in the presence of fatty acids (FAs). Additionally, Plin5 deficiency increased the NADH content and elevated the expression of lactate dehydrogenase (LDHA) in cardiomyocytes, which resulted in increased lactate production. Moreover, when fatty acid oxidation was blocked by etomoxir or LDHA was inhibited by GSK2837808A in Plin5-deficient cardiomyocytes, glucose utilization was improved. Leptin-deficient mice exhibited myocardial hypertrophy, insulin resistance and altered substrate utilization, and Plin5 deficiency exacerbated myocardial hypertrophy in leptin-deficient mice.

Conclusion

Our results demonstrated that Plin5 plays a critical role in coordinating fatty acid and glucose oxidation in cardiomyocytes, providing a potential target for the treatment of metabolic disorders in the heart.

Graphic abstract
Evaluation of the safety and immunological effects of Bacillus Calmette–Guérin in combination with checkpoint inhibitor therapy in a patient with neuroendocrine carcinoma: a case report
Authors: Föhse Konstantin , Debisarun Priya A. , Kilic Gizem , van Dodewaard-de Jong Joyce M. , Netea Mihai G.
Published: September 2023

Background

Immune checkpoint inhibitors have revolutionized therapy of advanced and metastatic cancers. However, a significant proportion of patients do not respond to immune checkpoint inhibitors or develop resistance. Therefore, novel therapies or combinations of therapies that may act synergistically are needed. It has been suggested that induction of trained immunity may increase the response to immune checkpoint inhibitor therapy, through reprogramming myeloid cells toward an antitumor phenotype. On the other hand, activation of the immune system also carries the risk of potentially sustaining tumorgenicity and increasing immune- related toxicity.

Case presentation

We report the case of a 37-year-old Dutch male suffering from gastric neuroendocrine carcinoma with liver metastases and high risk for an unfavorable outcome, who was treated with a combination of programmed cell death protein 1 inhibitor nivolumab and the trained immunity-inducer Bacillus Calmette–Guérin vaccine as a salvage therapy. Three doses of BCG vaccine were administered at 3-month intervals, in conjunction with the immune checkpoint inhibitor regimen. At a certain point, radiation therapy was added to the treatment regimen. During the combination of these therapies, the patient developed immune-mediated colitis, which necessitated discontinuation of all treatments. Bacillus Calmette–Guérin vaccination induced a trained immune response with elevated monocyte-derived interleukin-6 and interleukin-1β production capacity. From the first vaccination with Bacillus Calmette–Guérin until 3 months after the last vaccination with Bacillus Calmette–Guérin, the patient displayed only mild progression of the primary tumor and no progression of the metastases.

Conclusion

In this study, we show the feasibility to combine checkpoint inhibitor therapy with inducers of trained immunity in a patient with an aggressive neuroendocrine tumor. Autoimmune side effects are common under programmed cell death protein 1 inhibitor therapy, which was considered the most likely cause of colitis, although an additive effect of Bacillus Calmette–Guérin vaccination or radiotherapy cannot be excluded. The patient displayed only mild progression during the combination therapy, but larger studies are warranted to fully explore the potential benefit of trained immunity inducers as an adjuvant to immune checkpoint inhibitor therapy.
Factors affecting the use of dental services among Arab children in Israel: a qualitative study
Authors: Khatib Mohammad , Ashkenazi Yael , Loeff Yoav , Zusman Shlomo Paul , Natapov Lena
Published: September 2023

Background

In 2010, Israel reformed its hitherto dominantly privately financed dental services and included preventative and restorative dental care for children in the publicly-funded basket of healthcare services. A survey conducted by Brookdale Institute, found that only 67% of low-income Israeli-Arab children were using the new service (compared to 85% of Jewish children) while the majority of others continue using privately funded services. The aim of this study is to explore and explain Israeli-Arab children’s low utilization of publicly-funded preventive and restorative dental care.

Methods

A qualitative study designed to describe and understand the parents’ motivations and choices. As a preliminary stage, eight semi-structured interviews were conducted with directors of HMO dental departments and Israeli-Arab dentists. In the second stage, ten one-on-one interviews with parents, and five focus group discussions with 55 parents held on February–March 2017. All discussions in the focus groups were conducted in Arabic and each group was moderated by one of the research team accompanied by another person who documented and recorded the discussion. All interviews and discussions were recorded, transcribed in full and translated into Hebrew.

Results

The prevalent attitude is that one goes to the dentist only when there is a serious problem. The importance of preventive care is not appreciated. A childhood fear of the drill is very remembered and passed onto the children. Social and cultural factors such as kinship with service providers (GPs and dentists) influence the choice and utilization of health services. Economic barriers are still existing since even the small co-payment is daunting to low-income parents of large families. Provision of the public service is inadequate in some Arab villages.

Conclusions

The extension of Israel's National Health Insurance Law’s basket of services to include dental care for children, while important, is not sufficiently embraced by Israeli Arab children. The remaining barriers include poor living conditions, low educational level that requires very clear sharing of information about the new service, and the resistance of cultural and social traditions. Public dental services providers should focus on conveying relevant oral health messages to the parents too, either through their children or directly.
Antimicrobial stewardship in primary health care programs in humanitarian settings: the time to act is now
Authors: Truppa Claudia , Alonso Beatriz , Clezy Kate , Deglise Carole , Dromer Carole , Garelli Silvia , Jimenez Carolina , Kanapathipillai Rupa , Khalife Mohamad , Repetto Ernestina
Published: September 2023

Fragile and conflict-affected settings bear a disproportionate burden of antimicrobial resistance, due to the compounding effects of weak health policies, disrupted medical supply chains, and lack of knowledge and awareness about antibiotic stewardship both among health care providers and health service users. Until now, humanitarian organizations intervening in these contexts have confronted the threat of complex multidrug resistant infections mainly in their surgical projects at the secondary and tertiary levels of care, but there has been limited focus on ensuring the implementation of adequate antimicrobial stewardship in primary health care, which is known to be setting where the highest proportion of antibiotics are prescribed. In this paper, we present the experience of two humanitarian organizations, Médecins sans Frontières and the International Committee of the Red Cross, in responding to antimicrobial resistance in their medical interventions, and we draw from their experience to formulate practical recommendations to include antimicrobial stewardship among the standards of primary health care service delivery in conflict settings. We believe that expanding the focus of humanitarian interventions in unstable and fragile contexts to include antimicrobial stewardship in primary care will strengthen the global response to antimicrobial resistance and will decrease its burden where it is posing the highest toll in terms of mortality.
A hypoxia- and lactate metabolism-related gene signature to predict prognosis of sepsis: discovery and validation in independent cohorts
Authors: Peng Yaojun , Wu Qiyan , Ding Xinhuan , Wang Lingxiong , Gong Hanpu , Feng Cong , Liu Tianyi , Zhu Haiyan
Published: September 2023

Background

High throughput gene expression profiling is a valuable tool in providing insight into the molecular mechanism of human diseases. Hypoxia- and lactate metabolism-related genes (HLMRGs) are fundamentally dysregulated in sepsis and have great predictive potential. Therefore, we attempted to build an HLMRG signature to predict the prognosis of patients with sepsis.

Methods

Three publicly available transcriptomic profiles of peripheral blood mononuclear cells from patients with sepsis (GSE65682, E-MTAB-4421 and E-MTAB-4451, totaln = 850) were included in this study. An HLMRG signature was created by employing Cox regression and least absolute shrinkage and selection operator estimation. The CIBERSORT method was used to analyze the abundances of 22 immune cell subtypes based on transcriptomic data. Metascape was used to investigate pathways related to the HLMRG signature.

Results

We developed a prognostic signature based on five HLMRGs (ERO1L,SIAH2,TGFA,TGFBI, andTHBS1). This classifier successfully discriminated patients with disparate 28-day mortality in the discovery cohort (GSE65682,n = 479), and consistent results were observed in the validation cohort (E-MTAB-4421 plus E-MTAB-4451,n = 371). Estimation of immune infiltration revealed significant associations between the risk score and a subset of immune cells. Enrichment analysis revealed that pathways related to antimicrobial immune responses, leukocyte activation, and cell adhesion and migration were significantly associated with the HLMRG signature.

Conclusions

Identification of a prognostic signature suggests the critical role of hypoxia and lactate metabolism in the pathophysiology of sepsis. The HLMRG signature can be used as an efficient tool for the risk stratification of patients with sepsis.
Large-scale proximity extension assay reveals CSF midkine and DOPA decarboxylase as supportive diagnostic biomarkers for Parkinson’s disease
Authors: Paslawski Wojciech , Khosousi Shervin , Hertz Ellen , Markaki Ioanna , Boxer Adam , Svenningsson Per
Published: September 2023

Background

There is a need for biomarkers to support an accurate diagnosis of Parkinson’s disease (PD). Cerebrospinal fluid (CSF) has been a successful biofluid for finding neurodegenerative biomarkers, and modern highly sensitive multiplexing methods offer the possibility to perform discovery studies. Using a large-scale multiplex proximity extension assay (PEA) approach, we aimed to discover novel diagnostic protein biomarkers allowing accurate discrimination of PD from both controls and atypical Parkinsonian disorders (APD).

Methods

CSF from patients with PD, corticobasal syndrome (CBS), progressive supranuclear palsy (PSP), multiple system atrophy and controls, were analysed with Olink PEA panels. Three cohorts were used in this study, comprising 192, 88 and 36 cases, respectively. All samples were run on the Cardiovascular II, Oncology II and Metabolism PEA panels.

Results

Our analysis revealed that 26 and 39 proteins were differentially expressed in the CSF of test and validation PD cohorts, respectively, compared to controls. Among them, 6 proteins were changed in both cohorts. Midkine (MK) was increased in PD with the strongest effect size and results were validated with ELISA. Another most increased protein in PD, DOPA decarboxylase (DDC), which catalyses the decarboxylation of DOPA (L-3,4-dihydroxyphenylalanine) to dopamine, was strongly correlated with dopaminergic treatment. Moreover, Kallikrein 10 was specifically changed in APD compared with both PD and controls, but unchanged between PD and controls. Wnt inhibitory factor 1 was consistently downregulated in CBS and PSP patients in two independent cohorts.

Conclusions

Using the large-scale PEA approach, we have identified potential novel PD diagnostic biomarkers, most notably MK and DDC, in the CSF of PD patients.
Rosemary extract improves egg quality by altering gut barrier function, intestinal microbiota and oviductal gene expressions in late-phase laying hens
Authors: Zhang Lianhua , Ge Junwei , Gao Fei , Yang Min , Li Hui , Xia Fei , Bai Hongtong , Piao Xiangshu , Sun Zhiying , Shi Lei
Published: September 2023

Background

Rosemary extract (RE) has been reported to exert antioxidant property. However, the application of RE in late-phase laying hens on egg quality, intestinal barrier and microbiota, and oviductal function has not been systematically studied. This study was investigated to detect the potential effects of RE on performance, egg quality, serum parameters, intestinal heath, cecal microbiota and metabolism, and oviductal gene expressions in late-phase laying hens. A total of 210 65-week-old “Jing Tint 6” laying hens were randomly allocated into five treatments with six replicates and seven birds per replicate and fed basal diet (CON) or basal diet supplemented with chlortetracycline at 50 mg/kg (CTC) or RE at 50 mg/kg (RE50), 100 mg/kg (RE100), and 200 mg/kg (RE200).

Results

Our results showed that RE200 improved (P < 0.05) Haugh unit and n-6/n-3 of egg yolk, serum superoxide dismutase (SOD) compared with CON. No significant differences were observed for Haugh unit and n-6/n-3 of egg yolk among CTC, RE50, RE100 and RE200 groups. Compared with CTC and RE50 groups, RE200 increased serum SOD activity on d 28 and 56. Compared with CON, RE supplementation decreased (P < 0.05) total cholesterol (TC) level. CTC, RE100 and RE200 decreased (P < 0.05) serum interleukin-6 (IL-6) content compared with CON. CTC and RE200 increased jejunal mRNA expression of ZO-1 and Occludin compared with CON. The biomarkers of cecal microbiota and metabolite induced by RE 200, including Firmicutes, Eisenbergiella, Paraprevotella, Papillibacter, and butyrate, were closely associated with Haugh unit, n-6/n-3, SOD, IL-6, and TC. PICRUSt2 analysis indicated that RE altered carbohydrate and amino acid metabolism of cecal microbiota and increased butyrate synthesizing enzymes, including 3-oxoacid CoA-transferase and butyrate-acetoacetate CoA-transferase. Moreover, transcriptomic analysis revealed that RE200 improved gene expressions and functional pathways related to immunity and albumen formation in the oviductal magnum.

Conclusions

Dietary supplementation with 200 mg/kg RE could increase egg quality of late-phase laying hens via modulating intestinal barrier, cecal microbiota and metabolism, and oviductal function. Overall, RE could be used as a promising feed additive to improve egg quality of laying hens at late stage of production.

Graphical Abstract
68Ga-PSMA PET/CT-based multivariate model for highly accurate and noninvasive diagnosis of clinically significant prostate cancer in the PSA gray zone
Authors: Yang Jinhui , Li Jian , Xiao Ling , Zhou Ming , Fang Zhihui , Cai Yi , Tang Yongxiang , Hu Shuo
Published: September 2023

Background

The prostate-specific antigen (PSA) has been widely used in screening and early diagnosis of prostate cancer (PCa). However, in the PSA grey zone of 4–10 ng/ml, the sensitivity and specificity for diagnosing PCa are limited, resulting in considerable number of unnecessary and invasive prostate biopsies, which may lead to potential overdiagnosis and overtreatment. We aimed to predict clinically significant PCa (CSPCa) by combining the maximal standardized uptake value (SUVmax) based on 68Ga‑PSMA PET/CT and clinical indicators in men with gray zone PSA levels.

Methods

81 patients with suspected PCa based on increased serum total PSA (TPSA) levels of 4 − 10 ng/mL who underwent transrectal ultrasound/magnetic resonance imaging (MRI)/PET fusion-guided biopsy were enrolled. Among them, patients confirmed by histopathology were divided into the CSPCa group and the non-CSPCa group, and data on PSA concentration, prostate volume (PV), PSA density (PSAD), free PSA (FPSA)/TPSA, Prostate Imaging-Reporting and Data System version 2.1 (PI-RADS v2.1) score, 68Ga-PSMA PET/CT imaging evaluation results and SUVmax were compared. Multivariate logistic regression analysis was performed to identify the independent predictors for CSPCa, thereby establishing a predictive model based on SUVmax that was evaluated by analyzing the receiver operating characteristic (ROC) curve and decision curve analysis.

Results

Compared to non-CSPCa, CSPCa patients had smaller PVs (median, 31.40 mL), lower FPSA/TPSA (median, 0.12), larger PSADs (median, 0.21 ng/mL2) and higher PI-RADS scores (P < 0.05). The prediction model comprising 68Ga-PSMA PET/CT maximal standardized uptake value, PV and FPSA/TPSA had the highest AUC of 0.927 compared with that of other predictors alone (AUCs of 0.585 for PSA, 0.652 for mpMRI and 0.850 for 68Ga-PSMA PET/CT). The diagnostic sensitivity and specificity of the prediction model were 86.21% and 86.54%, respectively.

Conclusion

Given the low diagnostic accuracy of regular PSA tests, a new prediction model based on the 68Ga-PSMA PET/CT SUVmax, PV and FPSA/TPSA was developed and validated, and this model could provide a more satisfactory predictive accuracy for CSPCa. This study provides a noninvasive prediction model with high accuracy for the diagnosis of CSPCa in the PSA gray zone, thus may be better avoiding unnecessary biopsy procedures.
Space and atmospheric physics on Svalbard: a case for continued incoherent scatter radar measurements under the cusp and in the polar cap boundary region
Authors: Baddeley Lisa , Lorentzen Dag , Haaland Stein , Heino Erkka , Mann Ingrid , Miloch Wojciech , Oksavik Kjellmar , Partamies Noora , Spicher Andres , Vierinen Juha
Published: September 2023

Incoherent scatter radars (ISRs) represent the only instrument (both ground and space based) capable of making high temporal and spatial resolution measurements of multiple atmospheric parameters—such as densities, temperatures, particle velocities, mass flux—over an altitude range covering the entire mesosphere/lower thermosphere/ionosphere (MLTI) system on a quasi-continuous basis. The EISCAT Svalbard incoherent scatter radar (ESR), located just outside Longyearbyen (78.15$$^\circ$$

∘

N) on Svalbard, is the only currently operating facility capable of making such measurements inside the polar cusp—an area of significant energy input into the atmosphere and characterized by heating instabilities and turbulence. The ESR was built in the mid-1990s and has provided valuable data for the international experimental and modelling communities. New radar technologies are now available, in the form of phased array systems, which offer new data products and operational flexibility. This paper outlines the achievements and current research focus of the ESR and provides scientific arguments, compiled from inputs across the international scientific community, for a new phased array ISR facility on Svalbard. In addition to the fundamental scientific arguments, the paper discusses additional benefits of continued ISR observations on Svalbard, building on the key findings of the ESR. Svalbard has a large network of complementary instrumentation both focused on the MLTI system (e.g. the Kjell Henriksen auroral Observatory, the Svalbard SuperDARN radar and the Svalrak sounding rocket launch facility) with synergies to other research fields, such as meteorology and oceanography. As a further holistic system science view of the Earth becomes more important, a new ISR on Svalbard will be important also in this respect with its ability to provide datasets with a wide range of scientific applications. Increased activity in space has highlighted problematic issues such as space debris. A changing Arctic has also seen increased human activity via the opening up of new shipping routes, which are reliant on GNSS technology that is effected by severe turbulence in the MLTI system. As such, societal applications of a future ISR are also presented. The accessibility and logistical support for such a facility is also briefly discussed.
Stressful life events during the perimenopause: longitudinal observations from the seattle midlife women’s health study
Authors: Thomas Annette Joan , Mitchell Ellen Sullivan , Pike Kenneth C. , Woods Nancy Fugate
Published: September 2023

Background

Midlife is a time of increased responsibilities for women who have multiple roles including taking care of children, caring for elderly parents, managing households, and working outside the home. With little time for themselves, women additionally experience stressful life events (SLEs). The purpose of this study was to describe the longitudinal patterns of SLEs of women during midlife and to identify predictors of the SLE longitudinal patterns using baseline data of socio-economic factors and demographic characteristics.

Methods

Women who were part of the Seattle Midlife Women’s Health Study (SMWHS), a longitudinal study spanning more than 23 years, who had SLEs measured at baseline and at years 2, 7, and 10 were included in these analyses (N = 380 women at baseline). The Life Event Scale (LES), a 70-item scale based on a yes/no response and a Likert-based scoring system with 0 (no effect) to 4 (large effect), was used to determine the total and impact scores of midlife women. The LES was adapted to midlife women from the Norbeck Scale for younger, pregnant women. Analytic strategies consisted of a group-based trajectory model (GBTM) to examine subgroups of women with similar exposure to SLEs using socio-economic factors (gross family income, education, race/ethnicity, employment), demographic variables (age, marital status, being a parent), and menopausal transition stage to differentiate trajectories over time.

Results

Approximately 86% of women had medium high exposure to undesirable SLEs with a slight decrease (65.5%), or a sharp decrease (20.1%), over 10 years. The majority (approximately 64%) had moderate, sustained impact ratings, while approximately 35% had impact ratings that decreased over time. Most women (approximately 88%) reported desirable life events, which were sustained over the ten years, and which may help to balance or offset the high ratings of undesirable stressful life events. The rated impact of these desirable events decreased slightly over time for 65% of the sample. Socio-economic factors, demographic variables, and menopausal transition stages were not significant predictors of any of the four GBTMs.

Conclusion

Midlife women experience SLEs throughout the menopausal transition. Most of these midlife women had had a large amount of sustained stress over 10 years although all trajectories decreased to some extent over time. Since the menopausal transition stages were not significant predictors of the ratings of SLEs, a more complex set of factors, including social as well as biological, may explain the ratings of the women over the course of this ten-year observational study.
Adherence to the Mediterranean diet in young male soccer players
Authors: Leão César , Rocha-Rodrigues Sílvia , Machado Inês , Lemos João , Leal Sandra , Nobari Hadi
Published: September 2023

Introduction

Nutrition is vital in health and sports performance by improving anthropometric-related parameters and dietary habits, especially in the youngest ages. The Mediterranean diet (MD) has been highly recognized for its positive health effects and low adverse environmental impact.

Objectives

We aimed to characterize adherence to the MD and analyze its association with anthropometric parameters in young soccer players.

Methodology

In the present study, 132 male young soccer players from under 9 to under 15 categories (aged 7 to 15 years) from a Portuguese football club participated. The Mediterranean Diet Quality Index for Children and Adolescents (KIDMED) questionnaire was applied to assess adherence to the MD. Anthropometric-related parameters, including body mass, height, triceps skinfold thickness (TSKF), suprailiac skinfold thickness (SISKF), body mass index (BMI) and body fat percentage (%BF), were determined. The differences between groups were performed accordingly to normal and non-normal distribution. Spearman’s correlations were performed to analyze the hypothetical correlation between KIDMED and BMI.

Results

Players reached an average KIDMED score of 8.36 ± 1.92, showing that 68.2% (n = 90) reached high adherence to the MD, 31.1% (n = 41) had moderate adherence to the MD, and 0.78% (n = 1) had poor adherence to the MD. When the analysis was made for age group and BMI classification, no significant differences were observed in adherence to the MD. Considering the main characteristics of the MD, 50.8% consumed fruit (vs. 49,2%), 52.3% consumed vegetables (vs. 47,7%), and only 20% consumed oleaginous dried fruits (vs. 80%). Dairy consumption throughout the day was 49,2% (vs. no: 50,8%).

Conclusion

Data from the present study showed that many soccer players adhered to the MD, and no differences were observed for age group or BMI classification.
Polydopamine (PDA) coatings with endothelial vascular growth factor (VEGF) immobilization inhibiting neointimal formation post zinc (zn) wire implantation in rat aortas
Authors: Fu Jiayin , Zhu Qiongjun , Chen Zhezhe , Zhao Jing , Wu Shaofei , Zhao Meng , Xu Shihui , Lai Dongwu , Fu Guosheng , Zhang Wenbin
Published: September 2023

Background

Bioresorbable stents are designed to provide temporary mechanical support to the coronary arteries and then slowly degrade in vivo to avoid chronic inflammation. Zinc (Zn) is a promising material for bioresorbable stents; However, it can cause inflammation and neointimal formation after being implanted into blood vessels.

Methods

To improve biocompatibility of Zn, we first coated it with polydopamine (PDA), followed by immobilization of endothelial vascular growth factor (VEGF) onto the PDA coatings. Adhesion, proliferation, and phenotype maintenance of endothelial cells (ECs) on the coated Zn were evaluated in vitro. Then, a wire aortic implantation model in rats mimicking endovascular stent implantation in humans was used to assess vascular responses to the coated Zn wires in vivo. Thrombosis in aortas post Zn wire implantation, degradation of Zn wires in vivo, neointimal formation surrounding Zn wires, and macrophage infiltration and extracellular matrix (ECM) remodeling in the neointimas were examined.

Results

In vitro data showed that the PDA-coated Zn encouraged EC adhesion, spreading, proliferation, and phenotype maintenance on its surfaces. VEGF functionalization on PDA coatings further enhanced the biocompatibility of Zn to ECs. Implantation of PDA-coated Zn wires into rat aortas didn’t cause thrombosis and showed a faster blood flow than pure Zn or the Zn wires coated with VEGF alone. In addition, the PDA coating didn’t affect the degradation of Zn wires in vivo. Besides, the PDA-coated Zn wires reduced neointimal formation, increased EC coverage, decreased macrophage infiltration, and declined aggrecan accumulation in ECM. VEGF immobilization onto PDA coatings didn’t cause thrombosis and affect Zn degradation in vivo as well, and further increased the endothelization percentage as compared to PDA coating alone, thus resulting in thinner neointimas.

Conclusion

These results indicate that PDA coatings with VEGF immobilization would be a promising approach to functionalize Zn surfaces to increase biocompatibility, reduce inflammation, and inhibit neointimal formation after Zn implantation in vivo.

Graphical Abstract
Engaging community members to ensure culturally specific language is used in research: should I use gay, queer, MSM, or this other new acronym?
Authors: Rubini Kyle , Al-Bakri Taim , Bridel William , Clapperton Andrew , Greaves Mark , Hill Nolan E. , Labrecque Max , MacDonagh Richard , Miguel Glenndl , Orvis Shane , Osbourne-Sorrell Will , Randall Taylor , Reid Marco , Rosser Andrew , Presseau Justin , Vesnaver Elisabeth
Published: September 2023

Researchers often use terminology to define their participant groups that is rooted in a clinical understanding of the group’s shared identity(ies). Such naming often ignores the ways that the individuals who comprise these populations identify themselves. One oft-cited benefit of patient-oriented or community-engaged research is that language is local and relevant to impacted communities. This paper aims to contribute to the literature on how this local and relevant language can best be established. We ask how researchers can identify and implement accurate terminology, even when divergent perspectives exist within the communities involved. We draw from our experience with the Expanding Plasma Donation in Canada study, a community-engaged research study, which explored the views of people impacted by the “men who have sex with men” (MSM) blood donation policies in Canada. We describe the collaborative process through which we came to a consensual naming of this population, the challenges we faced, and a set of guiding principles we used to address them. We did not find an all-encompassing term or acronym that worked for all stages of research. Instead, we offer a set of guiding principles that can aid researchers engaging in a similar process: harm reduction, consent and transparency, collaboration and community involvement, recognition of missing voices, and resisting and/or restructuring oppressive standards.
Phubbing behavior, personality, and use of instagram by Brazilian adults: a correlational and predictive study
Authors: de Almeida Santos Adriane , de Oliveira Ferreira Breno , Leitão Consuelena Lopes , da Silva Iolete Ribeiro , de Souza Torres Marck
Published: September 2023

Phubbing behavior is a phenomenon that consists in ignoring people in situations of social interaction whilst paying attention to one’s smartphone. The study of this behavior enables reflection on the development of healthy behavior patterns when using technology and the design of intervention strategies to cope with phubbing behavior. This study aimed to investigate the relationship between phubbing behavior, use of Instagram, personality traits (Big Five), and sociodemographic variables (gender, education, and age) among Brazilian adults. This was a cross-sectional study with a sample of 1551 adults (61.7% women; 29.9% men), aged between 18 and 76 years (M = 31.6 years; SD = 9.6 years). The results of the correlation analysis indicated that excessive use of Instagram showed a high, positive association ρ (1551) = 0.442 with Phubbing Behavior and a moderate one with neuroticism ρ (1551) = 0.272. Phubbing behavior was positively and moderately with neuroticism ρ (1551) = 0.290, but it had a weak, negative correlation with age ρ (1551) = -0.117; p < 0.001. Multiple linear regression analysis (forward method) indicated that the variables that most strongly impacted Phubbing Behavior were neuroticism (ΔR2 = .236), conscientiousness (ΔR2 = .244) and use of Instagram (ΔR2 = .204). This result indicates that conscientiousness may have a predictive potential to decrease phubbing behavior, whereas neuroticism and use of Instagram may lead to increased phubbing. Multivariate Analysis of Variance indicated that excessive use of Instagram registered higher scores for women (M = 11.48; SD = 0.21) than for men (M = 9.45; SD = 0.27, p < 0.001). It was concluded that while conscientiousness can function as a protective factor for the development of phubbing behavior, high levels of neuroticism and excessive use of Instagram have greater potential to act as risk factors for it. In addition, neuroticism is also a risk factor for excessive use of Instagram, and women are more prone to such overuse.
Effect of a mattress on lumbar spine alignment in supine position in healthy subjects: an MRI study
Authors: Vitale Jacopo Antonino , Borghi Stefano , Bassani Tito , Messina Carmelo , Sconfienza Luca Maria , Galbusera Fabio
Published: September 2023

Background

Humans should sleep for about a third of their lifetime and the choice of the mattress is very important from a quality-of-life perspective. Therefore, the primary aim of this study was to assess the changes of lumbar angles, evaluated in a supine position using magnetic resonance imaging (MRI), on a mattress versus a rigid surface.

Methods

Twenty healthy subjects (10 females, 10 males), aged 32.3 ± 6.5 (mean ± standard deviation), with body mass index 22.4 ± 2.9, completed three evaluations: (i) spine MRI in supine position on a mattress (MAT); (ii) spine MRI in supine position on rigid surface (CON); and (iii) biplanar radiographic imaging in standing position. The following indexes were calculated for both MAT and CON: lumbar lordosis angles L1–L5, L1–S1, L5–S1, and the sacral slope (SS). Further, pelvic incidence (PI) was calculated from the biplanar radiographic images.

Results

Main findings were (i) L1–L5 and SS were greater in MAT than CON (L1:L5: +2.9°; SS: +2.0°); (ii) L5–S1 was lower in MAT than CON (−1.6°); (iii) L1–S1 was greater in MAT than CON only for male subjects (+2.0°); (iv) significant and positive correlations between PI and L1–L5, L1–S1 and SS were observed in both CON and MAT.

Conclusions

The use of a mattress determined small but statistically significant changes in lumbar angles.

Relevance statement

The use of a mattress determines small but statistically significant changes in radiological angles describing the sagittal alignment of the lumbar spine when lying in the supine position.

Key points

• Lordosis angle L1–L5 was greater in MAT than in CON condition (+2.9°).

• Sacral slope was greater in MAT than in CON condition (+2.0°).

• Lordosis angle L5–S1 was lower in MAT than in CON condition (−1.6°).

Graphical Abstract
Surgical intervention for the correction of fecal impaction in an obstipated cat with an old compression injury in the lumbosacral region of the spine
Authors: Munif Mohammad Raguib , Safawat Mst. Sanjida , Hannan Abdul
Published: September 2023

Background

Feline obstipation is a clinical condition often manifested by defecation inability that results in fecal impaction. This is frequently associated with a blunt traumatic injury that affects mostly the spine and surrounding structures in the caudal part of the back leading to vertebral compression.

Case presentation

A 6-month-old, female, mixed-breed cat of 1.49-kg BW was presented with a history of prolonged constipation (i.e., obstipation) along with discomfort and weakness. Prior to the presentation, the cat was medically treated with dietary fibers, fecal softeners, and enemas, but obstipation persisted. Clinical examinations revealed that there was straining but not effective for defecation. In addition, the cat had poor appetite and dehydration, electrolyte abnormalities, and defects in posture and gait. The lumbosacral region showed a depression that was supposed to be the consequence of an old, untreated regional injury. Radiographic imaging indicated a marked ventral curvature in the lumbosacral spine including major deviations in the positions of the L6 and L7 vertebrae and the sacrum as well. A firm tubular structure was felt on ventral abdominal palpation, and further ultrasonography revealed the tubular impaction. Laparotomy exposed a definite large bowel impaction, and enterotomy was performed to evacuate the firm feces. Postoperatively, supportive medications and care were provided for better recovery. However, the impaction recurred within 3 months after surgery, and euthanasia was a further choice.

Conclusions

Traumatic injuries involving the lumbosacral spine in cats might be serious enough to lead to severe constipation and thereby bowel impaction. Therefore, treatment should be prompt and accurate in any case of spinal injury to avoid further complications.
The relationship between coping self-efficacy and B cells in breast cancer patients
Authors: El-Amir Azza , El-Baiomy Eman M. , Sabry Noha A. , Kassem Loay , Chesney Margaret A. , Wallston Kenneth A.
Published: September 2023

Background

Breast cancer is the most common tumor among women throughout the world. Diagnosis and treatment of breast cancer are associated with stress and depression. Self-efficacy is one of the most important personal characteristics, studied in cancer, and is correlated with depression and immunity. The aim of the study is as follows:

1. Examining the correlation between coping self-efficacy with depression, DHEA levels, and immunity

2. Examining the correlation between depression and DHEA levels

3. Studying the effect of depression and DHEA levels on immunity

4. Examining the intermediate effect of DHEA levels on the correlation between coping self-efficacy and immunity

Methods

Thirty newly diagnosed breast cancer patients recruited from the Oncology Department, Kasr EL-Aini, Cairo University (ages 51.40 + 8.24 years) responded to two questionnaires: Coping Self-Efficacy Scale (CSES) and Patient Health Questionnaire-9 (PHQ-9); blood samples were collected to measure the phenotype of patients’ cellular immunity and DHEA levels by flowcytometry and ELISA technique.

Results

There was a significant negative correlation between CSES and PHQ-9, a significant positive correlation between PHQ-9 and B-cell count, and there is a significant negative correlation between CSES and B-cell count. The presence of DHEA has no mediatory role on correlation between CSES and B-cell count.

Conclusion

This paper presents a new model of psychoneuroimmunology by suggesting an effect of coping self-efficacy on immunity against breast cancer patients.
Evaluation of the impact of magnetic field homogeneity on image quality in magnetic resonance imaging: a baseline quantitative study at 1.5 T
Authors: Manson Eric Naab , Mumuni Abdul Nashirudeen , Inkoom Stephen , Shirazu Issahaku
Published: September 2023

Background

Magnetic resonance images can be affected in a number of ways by magnetic field inhomogeneity. The study aimed to optimize the main magnetic field homogeneity (MFH) by assessing how magnetic field inhomogeneity affects the signal-to-noise ratio (SNR) and geometric distortion of images acquired along the diameter of a spherical volume phantom from the isocenter of the MRI scanner.

Results

The MFH ranged between 0.10 and 0.60 ppm. The best MFH and the maximum SNR were determined in the isocenter at 400 mm field of view with the application of shim. However, for all the phantom positions, geometrical distortion in images acquired at 200 mm field of view was generally better and worse at 400 mm field of view. MFH could be optimized to reduce geometrical distortion and increase SNR by increasing the receiver bandwidth and the number of excitations whiles complementing it with shimming during image acquisition. According to Chi-square independent test, there were no significant differences (p > 0.05) in the MFH, SNR, and geometrical distortion values. Compared to findings at higher field strengths, it was observed that MRI systems of higher field strengths (greater than 1.5 T) could offer superior magnetic field homogeneity and SNR without causing observable geometrical distortion.

Conclusions

The optimal field of view for the fast field echo (FFE) sequence required to maximize MFH, SNR, and reduce distortion during image acquisition may vary across MRI systems of different field strengths. To determine the appropriate field of view, the method and results of this study could serve as a guide for medical physicists as part of their routine quality assurance test procedures.