bluuebunny/crossref_metadata_2025 huggingface dataset:
Protocol for single cell and single nucleus prep for Cell Pellets/Multiple Myeloma (snRNA & snATAC) v1
Authors: Jayasinghe Reyka
Published: August 2023

Protocol for single cell and single nucleus prep for Cell Pellets/Multiple Myeloma (snRNA & snATAC)
Human FFPE Liver Pathology Assessment -- University of Minnesota Human TMC v1
Authors: Adeyi Oyedele
Published: August 2023

Purpose: Describe the parameters evaluated per FFPE human liver sample received/obtained for the University of Minnesota SenNet TMC. These parameters are used to evaluate whether the tissue can be characterized as "normal" vs "diseased" prior to further protocols.
Mouse genetic models to manipulate enterochromaffin cell activity - Murine Organoid ELISA v1
Authors: Tohara Koki
Published: August 2023

This protocol describes how we maintain murine intestinal organoids and perform the enzyme-linked immunosorbent assay (ELISA) for serotonin (5-HT).
Mod3D Live Cell Chambers and holders 3D printing and Assembly v2
Authors: Ray Truant and
Published: August 2023

Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology laboratories. Consumer products in three-dimensional printing technology, for both Filament Deposition Modeling (FDM) and Masked Stereo Lithography (MSLA), have resulted in more biomedical research labs adopting the use of these devices for prototyping and manufacturing of lab plastic-based items, but rarely consumables. Here we describe a modular, live-cell chamber with multiple design options that can be mixed per experiment. Single reusable carriers and the use of biodegradable plastics, in a hybrid of FDM and MSLA manufacturing methods, reduce plastic waste. The system is easy to adapt to bespoke designs, with concept-to-prototype in a single day, offers significant cost savings to the users over commercial sources, and no loss in dimensional quality or reliability. (The last step in this version contains a supplemental video with extra context and tips, as part of the protocols.io Spotlight series, featuring conversations with protocol authors.)
Donor Selection Criteria for Adipose Procurement -- University of Minnesota Human TMC v1
Authors: Ikramuddin Sayeed
Published: August 2023

This document outlines the inclusion and exclusion criteria for donors of adipose (visceral, subcutenous) and blood for the SenNet Consortium program from the University of Minnesota Human TMC (Niedernhofer). Excerpt from the UMN IRB for Human Adipose procurement, section 8.0 Protocol title: Profiling of Adipose Tissue Depots and Immune Correlates Last updated: 2/10/2023
Typology of Physical Activity v1
Authors: Roberts Christine
Published: August 2023

Different physical activity types vary in metabolic demand (intensity), but also in non-metabolic demand (balance, co-ordination, speed and flexibility), cognitive demand (attention, memory and decision making), and social demand (social interaction). Activity types with different demands may have different effects on health outcomes but this cannot be formally tested until such demands can be reliably quantified. The present Delphi expert consensus study aims to objectively quantify the cognitive, physical and social demands of different PA types and used these scores to create a formal activity demand typology. International experts will systematically rate common activity types in terms of their intrinsic cognitive, physical and social demands, until consensus is reached. Cognitive, physical and social demand scores will be combined to create an overall non-metabolic demand rating for each activity type.
Physical Properties of Tapioca Starch-based Film Indicators with Anthocyanin Extract from Purple Sweet Potato (Ipomea batatas L.) and Response to pH Changes
Authors: Safinta Nurindra Rahmadhia Safinta Nurindra Rahmadhia , Afist Azkiya Sidqi Afist Azkiya Sidqi , Yanas Anggana Saputra Yanas Anggana Saputra
Published: August 2023

Intelligent packaging comes in the form of interactive film indicator using a natural pigment compound sensitive to pH changes. The development of intelligent packaging as an indicator film by utilizing natural pigment compounds that are related to pH change and food safety is motivated by increased consumer awareness of food safety. Purple sweet potato (Ipomoea batatas L.) is the source of anthocyanin flavonoid compounds sensitive to pH changes, demonstrated by color changes in film indicators. This research aims to determine physical properties and the pH response of tapioca starch-based film indicators with anthocyanin extract variation from purple sweet potato. Purple sweet potato anthocyanin (PSPA) indicator film was made using tapioca starch as biopolymer by casting method with the addition of anthocyanin extract at concentrations of 0, 5, 10, and 15 g. Furthermore, this research is conducted to analyze the physical properties of the film, and response to pH changes of fresh cow milk, Gindara fish fillet and chicken sausage stored at 7 °C and 25 °C under 48-hour observation. The results showed that the film indicator thickness was 0.72-0.74 mm, tensile strength was 1.23-9.86 MPa, elongation was 14.83-55.74%, and water vapor permeability (WVP) was 1.32-1.78 × 10-14 kg.m/m2.s.Pa. The results of this study indicated that the PSPA indicator films have the potential to be used as smart packaging to monitor food freshness and quality for safe consumption. That was supported by the good physical properties of PSPA indicator films.
The Effect of Lunasin on Inhibition of KI67, BCL-2 and C-MYC Expression in Azoxymethane and Dextran Sodium Sulfate Induced Mice Colon
Authors: Kusmardi Kusmardi , Ahmat Rediansya Putra Ahmat Rediansya Putra , Ria Kodariah Ria Kodariah
Published: August 2023

Treatment of cancer using medicinal-plant based has been important due to minimal side effects, high efficiency and low cost. Lunasin from soybean is known as potential chemopreventive agent. This study aimed to study and investigate the proteins involved in the mechanisms of action of lunasin underlie its chemopreventive effects in Azoxymethane (AOM) and Dextran Sodium Sulfate (DSS) induced mice. A total 30 BLAB/c mice were separated into six groups. In five of the groups - a negative control group, positive control group, and three intervention groups - carcinogenesis was induced with AOM and DSS; the sixth group received no interventions. Lunasin were given in different doses of Low Dose Lunasin (75 mg/kgBW), Moderate Dose Lunasin (150 mg/kgBW), and High Dose Lunasin (200 mg/kgBW) to intervention groups. Immunohistochemistry was conducted to measure Ki67, C-myc, and Bcl-2 expressions from the distal colons of mice that had been sacrificed. The samples were microscopically assessed and photographed, and cell counts were performed using the Image J application. Further, the H-score method was used to quantify of Ki67, C-myc and Bcl-2 expressions. The results of this show that there is significant differences between the negative control and the intervention groups were found at the 75 mg/kgBW and 150 mg/kgBW (p < 0.05) lunasin dosage levels. This demonstrates that Lunasin inhibits proliferation and induces apoptosis in the colon mice induced by AOM and DSS.
Effect of Crocodile Oil (Crocodylus siamensis) on Brain Mitochondrial Protein Expression and Cognition in Male Rats
Authors: Srisuksai Krittika , Parunyakul Kongphop , Santativongchai Pitchaya , Ampawong Sumate , Tulayakul Phitsanu , Fungfuang Wirasak
Published: August 2023

Crocodile oil (CO) is rich in polyunsaturated (PUFAs) fatty acids. Diets rich in PUFAs can maintain mitochondrial function, which is important in signal transduction and survival of neuronal cells. We investigated the effects of CO on brain mitochondrial protein expression and cognitive function in male rats. Twenty-one rats were randomly divided into three groups: (1) control, (2) treated with CO (3 mL/kg), and (3) treated with palm oil (PO; 3 mL/kg). Animals received oral gavage once-daily for seven weeks. The parameters that were measured were food intake, energy intake, body weight, serum lipid profiles, cognitive behavior, brain mitochondrial architecture, brain mitochondrial expression, and hippocampal structure. In CO and PO groups, food intake decreased significantly compared with that in the control group (p<0.05), but energy intake, body weight, and lipid profiles were not affected. Spatial learning in the PO group decreased significantly compared with that in control and CO groups (p<0.05). Crocodile oil significantly decreased the percentage of abnormal mitochondria (p<0.05) and the expression of apoptotic marker (p<0.05) compared with those in the PO treatment but also increased energy production marker (p<0.05) compared with those in the control and PO treatment. Moreover, percentage of intact hippocampal cells was not different between CO and control groups, but neuronal cells were lost in the PO group (p<0.05). This study suggest that CO could enhance the brain energy production and maintain cognitive function. CO can be an alternative dietary oil for treating brain energy disorder in the future.
The Effect of Liquid Smoke Treatment on Physical Stability and Impact Toughness Chicken Feather Fiber (CFF) as Reinforcement in Composites
Authors: Mukhlis Muslimin Mukhlis Muslimin , Saleh Saleh , Seng Ahmad , Wirawan Willy Artha , harbelubun Mohammad Muzni , Rais Sandi , Muhammad Alfajri Isham Muhammad Alfajri Isham
Published: August 2023

The objective of this study was to determine the effect of liquid smoke treatment on chicken feather fibres (CFF) on the morphology and impact toughness of composites reinforced with CFF. The experiment was carried out by measuring the density of CFF, followed by immersion in liquid smoke for 1, 2, and 3 hours. All of the fibers, including one without the immersion treatment, were then inserted into the oven at a temperature of 40 °C for 30 min. The morphological assessments were done using a scanning electron microscope (SEM) and the impact test according to the ASTM D5942-96 standard on the Charpy method impact tester. The SEM results showed that the fibers without immersion had a multitude of impurities at the surface, while with immersion treatment, the fibers had a more clean and rough surface. Impact test results showed that the fiber-reinforced composite without treatment was 61,583 Kj/m2, while the fiber-reinforced composite with immersion treatment for 1, 2, and 3 hours increased by 63,894 Kj/m2, 71,061 Kj/m2, and 80,538 Kj/m2. Treatment for 3 hours significantly increased the strength of the composite by 30.78%.
Is Personality Key? Persuasive Effects of Prior Attitudes and Personality in Political Microtargeting
Authors: Decker Hannah , Krämer Nicole
Published: August 2023

Messages that are designed to match a recipient’s personality, as enabled by microtargeting, have been found to influence political reasoning and even voting intentions. We extended these findings by adding prior attitudes to a microtargeting setting. Specifically, we examined what role different microtargeting approaches play in political reasoning by conducting an online experiment with a 2 (extraverted vs. introverted communication) × 2 (attitude-congruent vs. attitude-incongruent statement) between-subject design (N = 368). In line with the assumptions of the theory of motivated reasoning, attitude position matching emerged as an effective microtargeting strategy, and attitude strength moderated the effect of attitude congruency on recipients’ evaluations of political ads. While extraverted messages had no direct effect, that was unrelated to attitude congruency, recipients’ level of extraversion moderated the effect of extraverted communication on their evaluation of an ad. Interestingly, the intention to vote was significantly higher when an attitude-incongruent statement was phrased in an introverted rather than an extraverted manner, suggesting that information that challenges prior attitudes might be more persuasive when it is delivered in a more temperate way. In sum, the study indicates that matching message with personality alone might not be the most effective microtargeting approach within democratic societies.
“Anti-Regime Influentials” Across Platforms: A Case Study of the Free Navalny Protests in Russia
Authors: Glazunova Sofya , Amadoru Malmi
Published: August 2023

The full-scale invasion of Ukraine by Russia in 2022 has put the future of the Russian opposition further at stake. The new limitations towards political, internet, and press freedoms have led to a severe disintegration of the anti-regime movement in Russia, including its leaders like Alexey Navalny. Digital platforms had previously hosted anti-Kremlin narratives online and played a role in the facilitation of Russian anti-regime protests. The latest scalable anti-regime rallies to date were the Free Navalny protests, caused by the imprisonment of Navalny in 2021. Digital platforms strengthened the voice of the Russian regime critics; however, their growing visibility online caused further suppression in the country. To understand this paradox, we ask which main anti-regime communicators were influential in the protests’ discussions on Twitter, YouTube, and Facebook, and how platform features have facilitated their influence during the Free Navalny protests. We develop a multi-platform methodological workflow comprising network analysis, social media analytics, and qualitative methods to map the Russian anti-regime publics and identify its opinion leaders. We also evaluate the cultures of use of platforms and their features by various Russian anti-regime communicators seeking high visibility online. We distinguish between contextual and feature cultures of platform use that potentially aid the popularity of such actors and propose to cautiously confer the mobilisation and democratisation potential to digital platforms under growing authoritarianism.
Why We Should Distinguish Between Mobilization and Participation When Investigating Social Media
Authors: Haßler Jörg , Magin Melanie , Russmann Uta
Published: August 2023

In the recent past, social media has become a central channel and means for political and societal mobilization. Mobilization refers to the process by which political parties, politicians, social movements, activists, and other political and social actors induce citizens to participate in politics in order to win elections, convince others of their own positions, influence policies, and modify rulings. While not sufficient on its own for facilitating participation, mobilization is necessary for participation to occur, which justifies examining mobilization specifically to understand how people can be involved in politics. This thematic issue of Media and Communication presents various perspectives on the role of social media in mobilization, embracing both its recruitment side (traditional and non-established political actors, social and protest movements) and its network side (the ways citizens respond to mobilization appeals). Taken together, the thematic issue highlights the multifaceted nature and scholarly fruitfulness of mobilization as an independent concept.
Exploring Inclusive Cities for Migrants in the UK and Sweden: A Scoping Review
Authors: Ramachandran Niroshan , Di Matteo Claudia
Published: August 2023

In recent years, social work with migrants and ethnic minorities has developed as a field of research and practice. Further, it is recognised in the literature that the increased processes of human mobility in today’s societies have driven a growing focus on inclusive cities, especially in larger urban areas where ethnic diversity and cultural heterogeneity can be found alongside newly arrived migrants seeking a better quality of life, safety, and sanctuary. There is a strong link between individuals’ well‐being and their relationship with spaces, institutions, and resources. Cities and their urban environment have been increasingly identified as key arenas where social, economic, and ecological societal challenges should be addressed. In the context of migration, municipalities have invested in dealing with both inclusive and sustainable policies. However, cities are not uniformly experienced by all. This scoping review seeks to answer how an inclusive city is conceptualised in the Swedish and the UK’s social work literature concerning migration. Using social exclusion and inclusion as the theoretical points of view, we conduct analysis using Arksey and O’Malley’s (2005) six‐stage methodological framework. Despite social work playing a major role in the social inclusion of immigrant minorities in cities, through promoting participation, there is a lack of knowledge and research on social work engagement with social inclusion, both in the fields of social policy and practices. This article contributes to an enhanced understanding of what an inclusive city is, and the role of social work in defining and developing social policies and professional interventions for inclusive cities to support the integration of migrants with distinct needs. We offer a much‐needed review of the similarities and differences between the two geographies by analysing the social work perspectives from Sweden and the UK.
Exact pattern matching. Current achievements and research
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Zuiev Anton
Published: August 2023

The problem of exact pattern matching is an essential programming problem. Different algorithms that solve this problem are core elements of search engines, version control systems, text editors, DNA analyzers, and many others. For simplification reasons articles usually denote pattern as P or p and pattern length as M or m. Similarly, the text is usually denoted as T or t and its length - N or n. Alphabet is denoted Σ and its length - |Σ|. Based on these notations the problem of pattern matching can be written as follows: Find all positions/ amount of i, such that P[0...m] = T[i...i + m], or: Find all positions i in text for which substring starting at position i of the text of length m is equal to the pattern. The main parameters of this problem are pattern length and alphabet size. The length of the text usually doesn’t matter because, for any long enough text of a specific structure, the run time of the algorithm per character will be close to constant. Besides that, the specifics of the input data and text may also impact the performances of the algorithms. All of that makes the problem both very nuanced and interesting to investigate. This problem features a lot of different existing solutions developed over the course of the last 5 decades. The main part of the work provides short descriptions and analyses of a set of algorithms that are still relevant in the field. Besides that, some remarks are made on the topic of their theoretical regions of efficiency and how they depend on the specifics of the input. The results of the practical experimentation on the variety of randomly generated test data are provided. The conclusion provides some analysis of the received results and algorithms’ class efficiency based on the input as well as a visual representation of the received results in a form of a table representing the most efficient algorithm for each pair of pattern length and alphabet size.
Web applications as one of the modern ways of implementing decision support systems
Authors:   Taras Shevchenko National University of Kyiv, Kyiv, Ukraine, Ilkun O. P.
Published: August 2023

Decision support systems have a wide range of applications in various fields, including but not limited to commercial activities, medical institutions, educational institutions, scientific research, engineering, and government agencies. These systems support efficient processing of information from various sources using a variety of methodological approaches, including statistical analysis, data analysis, machine learning methods, optimization, and many others. In view of the above-mentioned features, there is an important issue of developing effective decision support systems that would meet the needs of modern organizations. This article reviews current approaches to software development, including analyzing a potential architecture for implementing a decision support system focused on patient diagnosis using fuzzy logic.
Trinitroaromatic explosives: Modern application, toxicological characterization, and methods of determination
Authors: Pogosyan Norayr G. , Shormanov Vladimir K. , Kvachakhiya Lekso L. , Omelchenko Vladimir A.
Published: August 2023

Explosives such as tetryl and picric acid, which were common in the past, now have lost their combat relevance. However, they are often used for peaceful purposes individually and in combination with other trinitroaromatic compounds (e.g., trinitrotoluene). As a result of their use, environmental pollution occurs, followed by intoxication of plants, animals, and people. Cases of explosive poisoning during their production are also described.
The symptoms of poisoning include both of general disorders and specific phenomena such as skin staining, impaired physiological efficiency of NADPh-dependent enzymes, genotoxicity, and immunotoxicity.
Previous scientific studies established a trend toward the development of chemical-analytical probes. Various options for the sensor surface of the device and methods for detecting compounds are considered. To determine the explosives, ion mobility spectrometry is widely used, which is very rare for the chemicaltoxicological analysis of other groups of compounds.
Simultaneously, methods commonly used in the analysis of narcotic and psychotropic substances (gas chromatography/ combination of high-performance liquid chromatography and mass spectrometry methods) are also applicable to determine trinitroaromatic explosives. However, the presence of nitro groups in their structure complicates such an analysis. This problem can be resolved by injecting cold samples directly to the column.
Despite the availability of various developed techniques and methods, the possibility of their application to study biological matrices remains insufficient.
Therefore, further studies of the chemicaltoxicological nature should be conducted to establish the optimal conditions for extracting the substances in question, the parameters of instrumental analysis, and the possibility of storing samples and for solving other problems of forensic medical examination.
Pedestrian electric-scooter fatality: A case report
Authors: Siedin Maxim S. , Plis Semyon S. , Klevno Vladimir A.
Published: August 2023

Electric scooters are one of the most popular means of individual mobility. The active use of this two-wheeled transport on sidewalks and public roads is naturally reflected in the number of road incidents, including fatal ones. No information has been provided about the damage received by pedestrians as a result of the collision of electric scooters, and the question of the most traumatic parts of the human body with such injuries today remains to be elucidated.
This case report describes a 75-year-old patient from practice that demonstrates the mechanism, type, and nature of injury sustained at a pedestrian when an electric scooter hits him. Citizen A sustained a head injury during the fall and was hospitalized in a medical institution, where he subsequently died. During autopsy, changes were found about the exact mechanism of their formation in a scooter injury.
According to the literature, injuries associated with the collision of electric scooters mainly occur during the fall with the formation of bone fractures in the upper and lower extremities. Torso injury, according to various authors, is extremely rare, and the nature of the damage and the category of victims in these studies are generally not detailed. This case confirms the possibility of the formation of primary and secondary contact injuries in various parts of the body of a pedestrian. In addition, the case allows the possibility of identifying the means of individual mobility based on the presence of a steering wheel.
Crushed amygdaloidal basalt rock and its effects on tomato production
Authors: Dalmora Adilson Celimar , Müller Kautzmann Rubens , Staubc Jair , Homrich Schneider Ivo André
Published: August 2023

Abstract

Sustainable agriculture plays an important role in agricultural productivity, in which it seeks to reduce dependence on conventional synthetic fertilizers (imported from Europe) and promote the use of alternative, low-cost and environmentally friendly sources. An experiment was carried out in the municipality of Santa Maria, Rio Grande do Sul, Brazil, with the objective of evaluating the efficiency and agronomic viability of using a mineral product (amygdaloid basalt powder) as an agricultural input on tomato productivity Solanum lycopersicum. The experimental design used was randomized blocks with six treatments and four replications, at doses 0 (treatment 1 - control), 1.0 ton ha-1 of rock dust (treatment 2), 2.5-ton ha-1 of rock dust (treatment 3), 3.5-ton ha-1 of rock dust (treatment 4), 4.5-ton ha-1 of rock dust (treatment 5), and recommended fertilization for tomato 5-20-20 NPK (treatment 6 - standard). The treatments were applied in December 2018 and the transplant took place in February 2019. According to the conditions under which the experiment was submitted and analyzing the results obtained, it can be inferred that the application of 1-ton ha-1 of amygdaloidal basalt in the soil increased parameters such as root length, stem diameter, green and dry mass of shoots and roots, number of flowers/plants, number of fruits/plant and productivity in tomato Solanum lycopersicum. It is a viable, sustainable, and low-cost strategy that contributes to the achievement of the Sustainable Development Goal (SDG 2) and can be replicated in Brazil and worldwide.
Van İlindeki Süt Sığırcılığı İşletmelerinin Sosyo Demografik ve İşletmecilik Özelliklerinin Karşılaştırılması
Authors: TERİN Mustafa , CEYLAN Melike , ÇİFTÇİ Kenan , YILDIRIM İbrahim
Published: August 2023

Bu araştırmada, Van İli Damızlık Sığır Yetiştiricileri Birliğine (VDSYB) üye olan ve olmayan süt sığırcılığı işletmelerinin sosyo-demografik ve işletmecilik yapılarının karşılaştırılması amaçlanmıştır. Araştırmanın materyalini, Van İli Damızlık Sığır Yetiştiricileri Birliğine üye olan 89 ve üye olmayan 89 işletmeden toplanan anket verileri oluşturmuştur. İşletmelerin sosyo demografik ve işletmecilik yapılarının karşılaştırılmasında parametrik ve non parametrik testler kullanılmıştır. İşletmelerin sosyo demografik özellikleri; yaş, eğitim durumu ve deneyim bakımından önemli farklılıkların olmadığı belirlenmiştir. Ancak işletmecilik yapıları bakımından; traktöre sahip olma, inek sayısı, süt verimi, sağım öncesi meme temizliği yapma, işletmede kayıt tutma, tarımsal desteklerden faydalanma, silaj ve hayvan hayat sigortası yaptırma değişkenleri bakımından birliğe üye olan ve olmayan işletmeler arsında önemli farklılıkların olduğu tespit edilmiştir. Bu sonuçlara göre, VDSYB’ne üye olan süt sığırcılığı işletmelerinin göreceli olarak daha bilinçli ve daha iyi üretim olanaklarına sahip olduğu söylenebilir. Bu sonuçlar tarımsal örgütlerin üyelerine ve ortaklarına doğrudan veya dolaylı olarak önemli katkılar sağladığını göstermiştir.
Associations of the VDR gene with clinical manifestations and complications of cystic fibrosis
Authors: Loshkova E. V. , Kondratyeva E. I. , Zhekaite E. K. , Klimov L. Ya. , Ilyenkova N. A. , Melyanovskaya Yu. L. , Voronkova A. Yu.
Published: August 2023

Cystic fibrosis (CF) is the most common severe autosomal recessive disease in the Caucasoid population caused by mutations in the CF transmembrane regulator (CFTR) gene. However, the course of the disease may be modulated by genetic factors other than the CFTR gene and may be pleiotropically influenced by VDR (Vitamin D Receptor) gene. The aim of the study was to search for associations between genetic variants (c.1206T>C(A>G), c.152T>C, c.1174+283G>A) of VDR gene and clinically significant manifestations of CF, complications, and responses to therapy. Methods. Patients with CF (n = 283) and healthy children (n = 333), who formed the control group, were examined. Calcidiol levels were tested in all subjects. Polymorphic variants of VDR gene (c.1206T>C(A>G), c.152T>C, c.1174+283G>A) were tested by polymerase chain reaction and restriction fragment length polymorphism analysis. Results. It was found that carriers of the TT genotype of the c.152T>C FokI variant of VDR gene are 6.3 times more likely to develop meconium ileus (odds ratio – OR – 6.375; p = 0.011), 3.2 times more likely – respiratory failure (OR – 3.253; p = 0.079), 3.4 times more likely – chronic lung infection (CIL) caused by Pseudomonas aeruginosa (OR – 3.432; p = 0.026), and 4 times more likely – CIL caused by non-fermenting gram-negative bacteria (OR – 4.056; p = 0.009). Carriers of the CC genotype of the c.1206T>C(A>G) TaqI genetic variant use systemic corticosteroids more frequently (66% vs 7%) (OR – 0.034; p = 0.001). It was shown that the AA genotype of the BsmlI polymorphism (c.1174 + 283G>A) is 4 times more likely to be detected in children with CF-associated liver diseases (OR – 4.300; p = 0.051). Conclusion. The contribution of all studied genetic variants c.1206T>C(A>G) TaqI, c.152T>C FokI, BsmlI (c.1174+283G>A) of the VDR gene to the clinical manifestations, complications and response to therapy in CF is described.
Assessment of long-term clinical and functional changes in patients recovering from severe COVID-19-associated lung damage
Authors: Abdullaeva G. B. , Avdeev S. N. , Fominykh E. V. , Gordina G. S. , Mustafina M. Kh.
Published: August 2023

The problem of long COVID-19 (COronaVIrus Disease 2019) has been highly relevant for the healthcare system in the last three years. The persistence of respiratory symptoms, radiological and functional changes in COVID-19 patients brings new challenges to the entire medical community. The aim of the study is to explore long-term clinical and functional changes in patients with severe COVID-19-associated lung injury, including assessment of functional and radiological abnormalities of the respiratory system, as well as persistent clinical symptoms a year after the acute phase of the disease. Methods. The study included 45 patients who were examined 3, 6 and 12 months after COVID-19 with severe lung damage (more than 50% according to chest CT in the acute phase of the disease). Patients underwent multispiral computed tomography of the chest organs, a comprehensive study of respiratory function (spirography, body plethysmography and diffusion test); the clinical symptoms were assessed. Results. Chest CT scans showed gradual regression of pathological changes during the follow-up. However, radiographic changes of varying severity persisted after 12 months of follow-up in 51% of patients. A year later, restrictive disorders persisted in 20% of patients and the diffusion capacity of the lungs was reduced in 69% of patients. At the same time, a statistically significant difference in the DLСО level was observed between 3, 6 and 12 months. The severity of dyspnea decreased 1 year after hospitalization in 48% of patients. Conclusion. The obtained results demonstrate a gradual regression of both radiological and functional pathological changes during the 1st year. However, CT changes and deviations of the respiratory function persist in some patients, mainly in the form of a decrease in DLСО, which necessitates further monitoring of this group of patients.
Impact of early tocilizumab administration on the progression of respiratory failure in COVID-19 patients
Authors: Merzhoeva Z. M. , Yaroshetskiy A. I. , Savko S. A. , Krasnoshchekova A. P. , Mandel I. A. , Tsareva N. A. , Trushenko N. V. , Nuralieva G. S. , Avdeev S. N.
Published: August 2023

The optimal interval for initiating tocilizumab therapy in patients with COVID-19 (COronaVIrus Disease 2019) has not been determined. The aim of the study was to evaluate the effectiveness of prescribing tocilizumab depending on the duration of persistent hyperthermia > 38 °С in patients with SARS-CoV-2 (Severe Acute Respiratory Syndrome-related CoronaVirus 2) associated pneumonia who received tocilizumab according to the Interim Guidelines of the Ministry of Health of the Russian Federation (version at the time of inclusion in the study). Methods. A retrospective cohort study was conducted in hospitalized patients (n = 163) with SARS-CoV-2-associated pneumonia from May 2020 to May 2021. Patients were retrospectively divided into 2 groups depending on the time of tocilizumab administration: ≤ 7 days (n = 61) or ≥ 8 days (n = 102) from the disease onset. Results. Patients who received tocilizumab in the first 7 days had the lower need for CPAP (Continuous Positive Airway Pressure) therapy on day 3 after tocilizumab therapy (HR (Hazard Ratio) – 0.129 (0.039 – 0.430); p = 0.001), a higher probability of a decrease in the volume of lung lesions on computed tomography > 25% a week after the use of tocilizumab (HR – 1.065 (1.036 – 1.093); p = 0.001), the lower probability of hemoglobin oxygen saturation below 92% on day 3 (HR – 0.807 (0.750 – 0.869); p = 0.001), and day 7 (HR – 0.825 (0.772 – 0.883); p = 0.001) after tocilizumab therapy. If CPAP therapy was required on day 3 after administration of tocilizumab, each day of delay in prescribing the drug increased the risk of an adverse outcome 18-fold (HR – 18.24 (5.328 – 62.438); p = 0.001). The duration of hospitalization was significantly lower in the early group than in the late group (10 (8.5 – 15) vs 13.5 (10 – 18) days, respectively; p = 0.02). The mortality was similar (5 (8.2%) vs 6 (5.9%) patients, respectively; p = 0.748). Conclusion. The administration of tocilizumab in the first seven days from the onset of the disease in patients with COVID-19 who developed systemic inflammation and lung damage may prevent the need for escalation of respiratory support and accelerate recovery compared with the later tocilizumab administration.
Status, problems and directions of improvement of pulmonological care for patients with chronic obstructive pulmonary disease according to the results of multicenter medical and sociological study
Authors: Rizakhanova O. A. , Avdeeva M. V. , Nikitina L. Y. , Avdeev S. N.
Published: August 2023

An expert analysis of existing organizational approaches at different levels of pulmonological medical care is needed to develop measures to improve pulmonological care for patients with chronic obstructive pulmonary disease. Aims: To conduct a multicenter medical and sociological study among pulmonologists to find priority areas and optimal ways to improve the organization of medical care for patients with chronic obstructive pulmonary disease (COPD). Methods. 181 pulmonologists from 23 regions of the Russian Federation were surveyed. Results. Pulmonologists believe that the actual prevalence (59.7%), disability rate (49.7%) and mortality rate (39.2%) associated with COPD are higher than official statistics in Russia. The most significant problems of the organization of pulmonological care are: defects in the organization of medical care at different levels, including insufficient quality of medical care (95.6%); lower quality of outpatient medical care compared to the inpatient care (p < 0.001); insufficient subsidized drug provision (69.6%); lack of dispensary follow-up (58.1%); insufficient rehabilitation measures (56.4%); diagnostic errors (53.4%); insufficient preventive measures (52.3%); long distance between specialized medical institution and the patient’s place of residence (41.4%); lack of continuum between the pre-hospital and hospital care (39.2%); lack of effective patient routing (37.1%); the high need of the regions in the medical and social programs to reduce morbidity and mortality from COPD (92.8%); insufficient contribution of the medical community to the adherence of COPD patients to prevention, treatment and rehabilitation; inadequate legal framework governing pulmonary care for COPD patients (29.3%). According to 97.7% of pulmonologists, COPD should be classified as a socially significant disease. This will allow for upscaling the programs aimed at prevention of COPD and reduction of the mortality rate and disability rate associated with COPD. Conclusion. The priority direction in the development of pulmonological care in Russia is improvement of regulatory and legal support with the development of more effective approaches to the provision of medical care to patients with COPD at all levels.
Multisystem Inflammatory Syndrome in Children (MIS-C) of Asian Countries: A Mini-Literature Review on its Clinical Characteristics and Outcomes
Authors: Arifuddin Mehnaaz , Hazari Mohammed
Published: August 2023

COVID-19 pandemic though has reached endemic levels in most of the countries, it has left an indelible mark on the healthcare systems across the world. One of the emerging challenges faced by physicians and researchers all around the world is the increased incidence of Multisystem inflammatory syndrome in children (MIS-C). Most of the research work conducted till date focusses on the pathophysiology, management and treatment of this syndrome. Multisystem inflammatory syndrome in children has been found to be a consequence of hyperactive immune system resulting from cytokine activation and release of immune complexes subsequent to COVID-19 infection. This condition is also associated with multisystem dysfunction which if not diagnosed early and not treated promptly, could result in an increased mortality among children. Most of the cases have been reported from European and American countries, but not many from Asia. This literature review provides for plausible reasons as to why the incidence of multisystem inflammatory syndrome in children has been less in Asian countries compared to the rest of the world. It also gives insights into the treatment protocols for multisystem inflammatory syndrome in children followed by hospitals in these countries and also highlights how different MIS-C is from Kawasaki disease in terms of clinical presentation since both these conditions share a common disease spectrum. This review also lists out the clinical features and treatment followed in such patients belonging to Asian countries.
Two-year-old infants’Mathematics Experiences
Authors:   The Korea Association of Yeolin Education, Kim Sungmi , Kim Wheetai
Published: August 2023

This study tried to explore the contexts of young children’s mathematics experiences with peers during block play activities at the classroom in a child care center. Seven two-year-old infants were observed for eight months in their classroom. This study found several findings of infants’ mathematics experiences with peers during their free play activities as follows: first, interests in peer play set off the mathematics experience of infants during free play, second, infants with competition during play with peers connected to the mathematics experiences, third, peers’ various information on mathematics during play made the infants mathematics experience during play, and forth, infants’ everyday conversation with peers was an important context to get any mathematics experience. This study implies that the results will be useful for making basic data and information for teachers to interact with infants on mathematics communication and experiences with peers. Also this study will be a great help for supplying teachers for infants to interact with peers on mathematics experiences.
Influência dos agregados reciclados miúdo e graúdo nas propriedades mecânicas do concreto autoadensável
Authors: Küster Junior Jocimar Souza , Ferreira Fernanda Giannotti da Silva , Almeida Filho Fernando Menezes de
Published: August 2023

O concreto autoadensável possui características específicas que o difere do concreto convencional. Destaca-se a sua capacidade de autoadensamento, facilidade de bombeamento, excelente homogeneidade e resistência à segregação, sendo estas características obtidas pela sua composição. Por outro lado, a reciclagem dos resíduos da construção civil vem despertando grande interesse e se consolidando como uma prática importante para a sustentabilidade do setor construtivo, pela redução do impacto ambiental gerado. Assim, o presente estudo buscou avaliar a influência da substituição dos agregados naturais por reciclados na produção de concreto autoadensável. Foram realizados ensaios no estado fresco para verificação da autoadensabilidade dos concretos. No estado endurecido, foram realizados ensaios para determinação da resistência à compressão simples, módulo de elasticidade, absorção de água, índice de vazios e massa específica. Os concretos produzidos apresentaram desempenho satisfatório no estado fresco, sendo considerados autoadensáveis. Quanto ao comportamento mecânico, os concretos apresentaram resistência à compressão similar ou superior ao concreto de referência. Ocorreu redução no módulo de elasticidade à medida que se elevou o teor de incorporação dos agregados reciclados. Já os valores de absorção de água e índice de vazios cresceram à medida que se aumentou o teor de incorporação dos agregados reciclados.
Criptococose cutânea em cão: relato de caso
Authors: Beserra Emannuel Estevão , Vilar Sthephanie Maria Rosas , Silva Francisco Edson De Oliveira , Lira Israel Felix , Sousa Manoel Oliveira de , Fernandes Annielle Regina Da Fonseca
Published: August 2023

A criptococose é uma doença fúngica causada por diferentes variedades do complexo Cryptococcus neoformans. Trata-se de um microrganismo oportunista que acomete majoritariamente indivíduos imunocomprometidos. A patologia é multissistêmica e, por isso, os sinais clínicos são variados, sendo o envolvimento tegumentar pouco relatado. Há diferentes métodos de diagnóstico definitivo, que variam desde citologia das lesões e biópsias até testes sorológicos, moleculares e culturas fúngicas, sendo este último considerado o padrão ouro. Quanto ao tratamento, consiste em um processo longo que pode variar de semanas a meses, utilizando-se drogas que apresentam importantes efeitos colaterais, com os antifúngicos sistêmicos sendo os mais indicados. Este estudo objetivou relatar um caso dessa patologia em um cão atendido por uma clínica veterinária particular, destacando seus principais aspectos clínicos. O animal apresentava lesões ulcerativas nos membros torácico e pélvico esquerdos, com evolução de uma semana. O diagnóstico foi confirmado pela citologia direta, e o tratamento foi baseado no uso de itraconazol por via oral na dose de 10 mg/kg a cada 24 horas por 90 dias, além de hepvet – na base de meio comprimido a cada 24 horas durante todo o tratamento –, eritrós tabs – um tablete a cada 24 horas por 30 dias –, e terapia tópica com xampu Micodine a cada 3 dias.
Análisis de varianza y coeficiente de variación como criterios de repetibilidad de una estufa de cocción basada en gasificación de pellets
Authors: Gutiérrez Jonatan , Chica Edwin , Pérez Juan F.
Published: August 2023

En este trabajo se presenta un estudio de repetibilidad aplicado a una estufa de cocción basada en gasificación de pellets de madera (19.03 MJ/kg). El análisis es llevado a cabo mediante un protocolo de ebullición de agua (Water Boling Test, WBT 4.2.3) modificado. La repetibilidad de la estufa basada en gasificación se evaluó mediante 2 metodologías: coeficiente de variación (CV, %) y análisis de varianza (ANOVA), en función de variables respuesta, tales como eficiencia térmica ( , %), potencia (P, kW), consumo de combustible (FC, g/min), consumo específico de combustible (SFC, g/L), consumo específico de energía (SFEC, kJ/L) y consumo específico de energía por unidad de tiempo (SFCT, kJ/L-min). Mediante el análisis de CV se obtuvo un CV promedio global de 4.69% ± 0.87%. Por tanto, como el CV ≤ 5%, se concluye que la estufa de cocción basada en gasificación es repetible. Con respecto al análisis ANOVA, los valores P de las variables estudiadas fueron mayores a 0.05 (P>0.05), por lo tanto, se concluye que la estufa de gasificación es repetible con un nivel de confianza del 95%. Consecuentemente, se concluye la eficiencia térmica promedio alcanzada por la estufa es del ~29% ± 2.25%, con un nivel de confianza del 95%. Este valor de eficiencia térmica es comparable con otras estufas de cocción basadas en gasificación reportadas en la literatura con valores entre 16 y 38%. La metodología y los resultados de este trabajo constituyen una base teórica para la evaluación de futuros desarrollos de estufas de cocción a biomasa, ya que se puede cuantificar y analizar la confiabilidad de los resultados encontrados en los prototipos probados a nivel de laboratorio.
Uso de los tiempos narrativos en la prensa escrita en francés y en español
Authors: Azpiazu Susana
Published: August 2023

A partir del análisis de diversos textos escritos de la prensa francesa y española que narran eventos actuales o lejanos en el tiempo, intentamos determinar el alcance de la deriva aorística del perfecto compuesto en el estándar de ambas lenguas, así como el modo en que se resuelve en ellas la sustitución del perfecto simple como forma prototípica de la narración. Comprobamos que en francés el proceso de deriva aorística se encuentra muy avanzado y da lugar a textos con un uso variado de las formas temporales (passé composé, imperfecto narrativo, presente histórico, participios, etc.) y distintos enfoques temporales, mientras que en español la tendencia es a preservar el uso narrativo clásico de las formas de anterioridad, con el perfecto compuesto como nueva forma narrativa especializada en eventos hodiernales y eventos en primer plano discursivo, pero sin desdeñar el perfecto simple en las narraciones de hechos más lejanos en el tiempo.
La traducción inglés > español de verbos de manera de movimiento en el subtitulado de documentales deportivos de Netflix enfocada en el aprendizaje de lenguas
Authors: López Martínez Leticia , Guerrero García Sandra
Published: August 2023

El presente trabajo se centra en explorar cuál es el grado de especificidad con el que se traducen los verbos de manera de movimiento del inglés al español en el ámbito de los deportes. Para ello se han utilizado dos documentales deportivos de la plataforma Netflix (Home Game y The Playbook) y se han analizado los subtítulos que ofrece la extensión Language Reactor en ambas lenguas. El objetivo es comprobar si es posible utilizar estas herramientas como recurso para el aprendizaje de patrones de lexicalización de movimiento en el proceso de adquisición de segundas lenguas. Se analiza del nivel de inclusión de información sobre la manera empleado por el traductor y los resultados apuntan a que la combinación de Netflix y Language Reactor no resulta un recurso conveniente para el aprendizaje de los patrones de lexicalización del movimiento. Sin embargo, sí supone una fuente de contenido didáctico para crear actividades de foco en la forma, método apropiado para este tipo de contenido gramatical más pormenorizado.
La réalisation des métaphores conceptuelles de la vaccination contre le COVID-19 en français et en tchèque
Authors: Mazurkiewicz Paulina , Lazar Jan
Published: August 2023

Cet article vise à analyser l’expression de la métaphore conceptuelle du vaccin contre le Covid-19 dans le discours médical de vulgarisation en français et en tchèque. Nous avons sélectionné, comme corpus de recherche, des sites Internet de vulgarisation médicale, en prenant en compte le sujet de la vaccination contre le Covid-19 (doctissimo.fr pour la langue française et www.idnes.cz pour la langue tchèque). Les recherches menées montrent un réseau de métaphores pour expliquer un phénomène complexe de lutte efficace contre le virus qui cause cette maladie. Les experts se réfèrent à la métaphore structurelle montrant le transfert des structures conceptuelles du domaine source OPÉRATION MILITAIRE vers le domaine cible VACCINATION. À son tour, le vaccin conceptualisé comme SOLDAT est un exemple d’anthropomorphisation dans une métaphore ontologique. L’examen du corpus comparable a montré une quantité importante d’équivalences lexicales et syntaxiques totales résultant d’une même perception de la réalité pandémique par les Tchèques et les Français. D’autre part, cela peut être dû à la référence à l’anglais, dans lequel la recherche scientifique mondiale s’exprime le plus souvent.