bluuebunny/crossref_metadata_2025 huggingface dataset:
The orphan nuclear receptor Nr4a1 mediates perinatal neuroinflammation in a murine model of preterm labor
Authors: Estrada Sarah M. , Thagard Andrew S. , Dehart Mary J. , Damicis Jennifer R. , Dornisch Elisabeth M. , Ippolito Danielle L. , Burd Irina , Napolitano Peter G. , Ieronimakis Nicholas
Published: January 2020

Prematurity is associated with perinatal neuroinflammation and injury. Screening for genetic modulators in an LPS murine model of preterm birth revealed the upregulation of Nr4a1, an orphan nuclear transcription factor that is normally absent or limited in embryonic brains. Concurrently, Nr4a1 was downregulated with magnesium sulfate (MgSO4) and betamethasone (BMTZ) treatments administered to LPS exposed dams. To understand the role of Nr4a1 in perinatal brain injury, we compared the preterm neuroinflammatory response in Nr4a1 knockout (KO) versus wild type (wt) mice. Key inflammatory factors Il1b, Il6 and Tnf, and Iba1+ microglia were significantly lower in Nr4a1 KO versus wt brains exposed to LPS in utero. Treatment with MgSO4/BMTZ mitigated the neuroinflammatory process in wt but not Nr4a1 KO brains. These results correspond with a reduction in cerebral hemorrhage in wt but not mutant embryos from dams given MgSO4/BMTZ. Further analysis with Nr4a1-GFP-Cre × tdTomato loxP reporter mice revealed that the upregulation of Nr4a1 with perinatal neuroinflammation occurs in the cerebral vasculature. Altogether, this study implicates Nr4a1 in the developing vasculature as a potent mediator of neuroinflammatory brain injury that occurs with preterm birth. It is also possible that MgSO4/BMTZ mitigates this process by direct or indirect inhibition of Nr4a1.
Epigenetic priming by Dot1l in lymphatic endothelial progenitors ensures normal lymphatic development and function
Authors: Yoo Hyunjin , Lee Young Jae , Park Chanhyeok , Son Dabin , Choi Dong Yoon , Park Ji-Hyun , Choi Hee-Jin , La Hyun Woo , Choi Yun-Jung , Moon Eun-Hye , Saur Dieter , Chung Hyung Min , Song Hyuk , Do Jeong Tae , Jang Hoon , Lee Dong Ryul , Park Chankyu , Lee Ok-Hee , Cho Ssang-Goo , Hong Seok-Ho , Kong Gu , Kim Jin-Hoi , Choi Youngsok , Hong Kwonho
Published: January 2020

Proper functioning of the lymphatic system is required for normal immune responses, fluid balance, and lipid reabsorption. Multiple regulatory mechanisms are employed to ensure the correct formation and function of lymphatic vessels; however, the epigenetic modulators and mechanisms involved in this process are poorly understood. Here, we assess the regulatory role of mouse Dot1l, a histone H3 lysine (K) 79 (H3K79) methyltransferase, in lymphatic formation. Genetic ablation of Dot1l in Tie2(+) endothelial cells (ECs), but not in Lyve1(+) or Prox1(+) lymphatic endothelial cells (LECs) or Vav1(+) definitive hematopoietic stem cells, leads to catastrophic lymphatic anomalies, including skin edema, blood–lymphatic mixing, and underdeveloped lymphatic valves and vessels in multiple organs. Remarkably, targeted Dot1l loss in Tie2(+) ECs leads to fully penetrant lymphatic aplasia, whereas Dot1l overexpression in the same cells results in partially hyperplastic lymphatics in the mesentery. Genetic studies reveal that Dot1l functions in c-Kit(+) hemogenic ECs during mesenteric lymphatic formation. Mechanistically, inactivation of Dot1l causes a reduction of both H3K79me2 levels and the expression of genes important for LEC development and function. Thus, our study establishes that Dot1l-mediated epigenetic priming and transcriptional regulation in LEC progenitors safeguard the proper lymphatic development and functioning of lymphatic vessels.
BCL-XL expression is essential for human erythropoiesis and engraftment of hematopoietic stem cells
Authors: Afreen Sehar , Bohler Sheila , Müller Alexandra , Demmerath Eva-Maria , Weiss Julia Miriam , Jutzi Jonas Samuel , Schachtrup Kristina , Kunze Mirjam , Erlacher Miriam
Published: January 2020

The anti-apoptotic BCL-2 proteins (BCL-2, BCL-XL, MCL-1, A1, BCL-W) counteract apoptotic signals emerging during development and under stress conditions, and are thus essential for the survival of every cell. While the “BCL-2 addiction” of different cell types is well described in mouse models, there is only limited information available on the role of different anti-apoptotic BCL-2 proteins in a given human cell type. Here we characterize the role of BCL-XL for survival and function of human hematopoietic cells, with the aim to predict hematological side effects of novel BCL-XL-inhibiting BH3-mimetics and to identify hematological malignancies potentially responsive to such inhibitors. Earlier clinical studies have shown that the combined BCL-2/BCL-XL/BCL-W inhibitor, Navitoclax (ABT-263) induces severe thrombocytopenia caused by direct platelet demise and counteracted by increased megakaryopoiesis. In contrast, murine studies have reported important contribution of BCL-XL to survival of late erythroid cells and megakaryocytes. Using lentiviral knockdown, we show that the roles of BCL-XL for human hematopoietic cells are much more pronounced than expected from murine data and clinical trials. Efficient genetic or chemical BCL-XL inhibition resulted in significant loss of human erythroid cells beginning from very early stages of erythropoiesis, and in a reduction of megakaryocytes. Most importantly, BCL-XL deficient human hematopoietic stem cells and multipotent progenitors were reduced in numbers, and they showed a severely impaired capacity to engraft in mice during xenotransplantation. BCL-XL deficiency was fully compensated by BCL-2 overexpression, however, loss of its antagonist BIM did not result in any rescue of human erythroid or stem and progenitor cells. We thus conclude that novel and specific BCL-XL inhibitors might be efficient to treat malignancies of erythroid or megakaryocytic origin, such as polycythemia vera, acute erythroid leukemia, essential thrombocytosis or acute megakaryocytic leukemia. At the same time, it can be expected that they will have more severe hematological side effects than Navitoclax.
Acyl-CoA-binding protein (ACBP): a phylogenetically conserved appetite stimulator
Authors: Charmpilas Nikolaos , Ruckenstuhl Christoph , Sica Valentina , Büttner Sabrina , Habernig Lukas , Dichtinger Silvia , Madeo Frank , Tavernarakis Nektarios , Bravo-San Pedro José M. , Kroemer Guido
Published: January 2020

Recently, we reported that, in mice, hunger causes the autophagy-dependent release of a protein called “acyl-CoA-binding protein” or “diazepam binding inhibitor” (ACBP/DBI) from cells, resulting in an increase in plasma ACBP concentrations. Administration of extra ACBP is orexigenic and obesogenic, while its neutralization is anorexigenic in mice, suggesting that ACBP is a major stimulator of appetite and lipo-anabolism. Accordingly, obese persons have higher circulating ACBP levels than lean individuals, and anorexia nervosa is associated with subnormal ACBP plasma concentrations. Here, we investigated whether ACBP might play a phylogenetically conserved role in appetite stimulation. We found that extracellular ACBP favors sporulation in Saccharomyces cerevisiae, knowing that sporulation is a strategy for yeast to seek new food sources. Moreover, in the nematode Caenorhabditis elegans, ACBP increased the ingestion of bacteria as well as the frequency pharyngeal pumping. These observations indicate that ACBP has a phylogenetically ancient role as a ‘hunger factor’ that favors food intake.
Oligodendrocyte precursor cells transplantation protects blood–brain barrier in a mouse model of brain ischemia via Wnt/β-catenin signaling
Authors: Wang Liping , Geng Jieli , Qu Meijie , Yuan Fang , Wang Yuyang , Pan Jiaji , Li Yongfang , Ma Yuanyuan , Zhou Panting , Zhang Zhijun , Yang Guo-Yuan
Published: January 2020

Blood–brain barrier damage is a critical pathological feature of ischemic stroke. Oligodendrocyte precursor cells are involved in maintaining blood–brain barrier integrity during the development. However, whether oligodendrocyte precursor cell could sustain blood–brain barrier permeability during ischemic brain injury is unknown. Here, we investigate whether oligodendrocyte precursor cell transplantation protects blood–brain barrier integrity and promotes ischemic stroke recovery. Adult male ICR mice (n = 68) underwent 90 min transient middle cerebral artery occlusion. After ischemic assault, these mice received stereotactic injection of oligodendrocyte precursor cells (6 × 105). Oligodendrocyte precursor cells transplantation alleviated edema and infarct volume, and promoted neurological recovery after ischemic stroke. Oligodendrocyte precursor cells reduced blood–brain barrier leakage via increasing claudin-5, occludin and β-catenin expression. Administration of β-catenin inhibitor blocked the beneficial effects of oligodendrocyte precursor cells. Wnt7a protein treatment increased β-catenin and claudin-5 expression in endothelial cells after oxygen–glucose deprivation, which was similar to the results of the conditioned medium treatment of oligodendrocyte precursor cells on endothelial cells. We demonstrated that oligodendrocyte precursor cells transplantation protected blood–brain barrier in the acute phase of ischemic stroke via activating Wnt/β-catenin pathway. Our results indicated that oligodendrocyte precursor cells transplantation was a novel approach to the ischemic stroke therapy.
Novel HDAC inhibitor Chidamide synergizes with Rituximab to inhibit diffuse large B-cell lymphoma tumour growth by upregulating CD20
Authors: Guan Xu-Wen , Wang Hua-Qing , Ban Wei-Wei , Chang Zhi , Chen Hai-Zhu , Jia Li , Liu Feng-Ting
Published: January 2020

Loss of CD20 is a major obstacle for the retreatment of relapsed/refractory diffuse large B cell lymphoma (DLBCL) with Rituximab-associated regimens. Histone deacetylation causes gene silencing and inhibits CD20 expression. Chidamide is a novel inhibitor for histone deacetylases (HDACs). We hypothesize that Chidamide could overcome Rituximab-mediated down-regulation of CD20 and facilitate Rituximab-induced killing. In this study, we determine the mechanism of synergy of Chidamide with Rituximab in DLBCL using in vitro and in vivo models. We found that the levels of CD20 protein surface expression on five DLBCL cell lines were significantly and positively correlated with the sensitivities of cells to Rituximab. Treatment with Rituximab significantly reduced CD20 surface expression at the protein levels. RNA sequencing showed that Chidamide significantly increased expression of more than 2000 transcriptomes in DLBCL cells, around 1000 transcriptomes belong to the cell membrane and cell periphery pathways, including MS4A1. Chidamide significantly increased CD20 surface expression in DLBCL cell lines. Combination with Chidamide significantly synergized Rituximab-induced cell death in vitro and significantly inhibited tumour growth in DLBCL-bearing xenograft mice. A patient with relapsed/refractory DLBCL achieved a complete response after three cycles combined treatment with Chidamide and Rituximab. In conclusion, our data demonstrate for the first time that inhibition of HDACs by Chidamide significantly enhanced Rituximab-induced tumour growth inhibition in vitro and in vivo. We propose that CD20 surface expression should be used clinically to evaluate treatment response in patients with DLBCL. Chidamide is a promising sensitizer for the retreatment of DLBCL with Rituximab.
Correction: Protease Nexin I is a feedback regulator of EGF/ PKC/MAPK/EGR1 signaling in breast cancer cells metastasis and stemness
Authors: Tang Tingting , Zhu Qinhua , Li Xinping , Zhu Gaole , Deng Siwei , Wang Yingshan , Ni Lingyu , Chen Xinyuan , Zhang Yanfeng , Xia Tiansong , Zen Ke , Pan Yi , Jin Liang
Published: January 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Off-target effect of the BMI1 inhibitor PTC596 drives epithelial-mesenchymal transition in glioblastoma multiforme
Authors: Flamier Anthony , Abdouh Mohamed , Hamam Rimi , Barabino Andrea , Patel Niraj , Gao Andy , Hanna Roy , Bernier Gilbert
Published: January 2020

Glioblastoma multiforme (GBM) is an incurable primary brain tumor containing a sub-population of cancer stem cells (CSCs). Polycomb Repressive Complex (PRC) proteins BMI1 and EZH2 are enriched in CSCs, promoting clonogenic growth and resistance to genotoxic therapies. We report here that when used at appropriate concentrations, pharmaceutical inhibitors of BMI1 could efficiently prevent GBM colony growth and CSC self-renewal in vitro and significantly extend lifespan in terminally ill tumor-bearing mice. Notably, molecular analyses revealed that the commonly used PTC596 molecule targeted both BMI1 and EZH2, possibly providing beneficial therapeutic effects in some contexts. On the other hand, treatment with PTC596 resulted in instant reactivation of EZH2 target genes and induction of a molecular program of epithelial–mesenchymal transition (EMT), possibly explaining the modified phenotype of some PTC596-treated tumors. Treatment with a related but more specific BMI1 inhibitor resulted in tumor regression and maintenance of cell identity. We conclude that inhibition of BMI1 alone is efficient at inducing GBM regression, and that dual inhibition of BMI1 and EZH2 using PTC596 may be also beneficial but only in specific contexts.
Optical biosensors: an exhaustive and comprehensive review
Authors: Chen Chen , Wang Junsheng
Published: January 2020

Optical biosensors have exhibited worthwhile performance in detecting biological systems and promoting significant advances in clinical diagnostics, drug discovery, food process control, and environmental monitoring.
The search for a unique Raman signature of amyloid-beta plaques in human brain tissue from Alzheimer's disease patients
Authors: Lochocki Benjamin , Morrema Tjado H. J. , Ariese Freek , Hoozemans Jeroen J. M. , de Boer Johannes F.
Published: January 2020

Raman spectroscopy was used to examine unstained, formalin fixed Alzheimer's disease human brain tissue to potentially identify a unique spectral signature of amyloid-beta plaques.
A flexible and highly sensitive nitrite sensor enabled by interconnected 3D porous polyaniline/carbon nanotube conductive hydrogels
Authors: Gao Fengxian , Teng He , Song Jingyao , Xu Guiyun , Luo Xiliang
Published: January 2020

A flexible and highly sensitive nitrite sensor based on PANI–CNT hydrogels.
From an organic ligand to a metal–organic coordination polymer, and to a metal–organic coordination polymer–cocrystal composite: a continuous promotion of the proton conductivity of crystalline materials
Authors: Liang Xiaoqiang , Cao Tingting , Wang Li , Zheng Changzheng , Zhao Yamei , Zhang Feng , Wen Chen , Feng Lei , Wan Chengan
Published: January 2020

A new strategy was proposed to increase proton conductivities in metal–organic coordination polymers (MOCPs) commencing from organic ligands, i.e. coordination inducement and MOCP–cocrystal composite formation.
First-principles investigation of the electronic properties of the Bi2O4(101)/BiVO4(010) heterojunction towards more efficient solar water splitting
Authors: Cao Xiaofei , Zhao Xin , Hu Jun , Chen Zhong
Published: January 2020

Forming a Bi2O4/BiVO4 heterojunction can effectively improve the activity and stability of BiVO4 for water splitting reactions.
Enhanced photoresponse of epitaxially grown ZnO by MoO3 surface functionalization
Authors: Yang XiangDong , Wang HaiTao , Dou WeiDong , Wang Peng , Yang XuXin , Pan XinHua , Lu Bin , Mao HongYing
Published: January 2020

Enhanced photoresponse of epitaxially grown ZnO has been observed with MoO3 surface functionalization, which is attributed to the larger upward band bending at the interface.
Correction: Plasma-induced grafting of polyacrylamide on graphene oxide nanosheets for simultaneous removal of radionuclides
Authors: Song Wencheng , Wang Xiangxue , Wang Qi , Shao Dadong , Wang Xiangke
Published: January 2020

Correction for ‘Plasma-induced grafting of polyacrylamide on graphene oxide nanosheets for simultaneous removal of radionuclides’ by Wencheng Song et al., Phys. Chem. Chem. Phys., 2015, 17, 398–406.
Sol–gel synthesis of 2-dimensional TiO2: self-assembly of Ti–oxoalkoxy–acetate complexes by carboxylate ligand directed condensation
Authors: Sui Ruohong , Deering Connor E. , Prinsloo Rohen , Lavery Christopher B. , Chou Nancy , Marriott Robert A.
Published: January 2020

2-Dimensional TiO2 is synthesized for the first time by a sol–gel self-assembly of Ti–oxoalkoxy–acetate complexes.
In vitro digestibility and bioaccessibility of lipid-based delivery systems obtained via enzymatic glycerolysis: a case study of rosemary extract bioaccessibility
Authors: Corzo-Martínez Marta , Bañares Celia , Díaz Alejandro , Vázquez Luis , Reglero Guillermo , Torres Carlos F.
Published: January 2020

The effect of enzymatic glycerolysis on digestibility, bioaccessibility of ratfish liver oil rich in alkylglycerols, and the capability of the glycerolysis product to act as lipid-based delivery system for a supercritical rosemary extract was studied.
Effects of regular and decaffeinated roasted coffee (Coffea arabica and Coffea canephora) extracts and bioactive compounds on in vitro probiotic bacterial growth
Authors: Sales Amanda Luísa , dePaula Juliana , Mellinger Silva Caroline , Cruz Adriano , Lemos Miguel Marco Antônio , Farah Adriana
Published: January 2020

The aim of this study was to investigate the effects of coffee species, roast degree and decaffeination on in vitro probiotic bacterial growth, and to identify the major coffee compounds responsible for such effects.
Facile synthesis of highly fluorescent free-standing films comprising graphitic carbon nitride (g-C3N4) nanolayers
Authors: Yadav Ram Manohar , Kumar Rajesh , Aliyan Amir , Dobal Pramod S. , Biradar Santoshkumar , Vajtai Robert , Singh Dinesh Pratap , Martí Angel A. , Ajayan Pulickel M.
Published: January 2020

The free-standing g-C3N4 films were fabricated by thermal condensation of C2H4N4 at 600 °C in a low pressure of Ar atmosphere. The as-synthesized g-C3N4 films exhibited stable and strong photoluminescence emission centered around 455–460 nm.
Adhesion layer influence on controlling the local temperature in plasmonic gold nanoholes
Authors: Jiang Quanbo , Rogez Benoît , Claude Jean-Benoît , Moreau Antonin , Lumeau Julien , Baffou Guillaume , Wenger Jérôme
Published: January 2020

The gold adhesion layer can have a dramatic impact on the thermal response of plasmonic structures, offering new ways to promote or avoid the temperature increase in plasmonics.
Pt decorated POMOF-derived constructions for efficient electrocatalytic hydrogen evolution
Authors: Jia Wei , Zhang Juanli , Lu Zhenjiang , Wang Shiqiang , Feng Shizhan
Published: January 2020

A POMOF-assisted strategy was developed to synthesize Pt–Cu/Mo2C which features a low overpotential and superior stability toward electrocatalytic hydrogen evolution.
An artificial sea urchin with hollow spines: improved mechanical and electrochemical stability in high-capacity Li–Ge batteries
Authors: Liu Jinyun , Lin Xirong , Han Tianli , Lu Qianqian , Long Jiawei , Zhang Huigang , Chen Xi , Niu Junjie , Li Jinjin
Published: January 2020

Metallic germanium (Ge) as the anode can deliver a high specific capacity and high rate capability in lithium ion batteries.
Covalent organic framework-supported platinum nanoparticles as efficient electrocatalysts for water reduction
Authors: Park Eunsol , Jack Joshua , Hu Yiming , Wan Shun , Huang Shaofeng , Jin Yinghua , Maness Pin-Ching , Yazdi Sadegh , Ren Zhiyong , Zhang Wei
Published: January 2020

Well-dispersed and highly stable platinum nanoparticles, supported on a covalent organic framework, exhibit excellent catalytic activities toward hydrogen evolution reaction.
Spin curvature induced resistivity in epitaxial half-metallic CrO2 thin films
Authors: Qian Lijuan , Chen Wenzhe , Xiao Gang
Published: January 2020

This work reports the quantitative relationship between spin curvature and the corresponding magnetoresistance through transport measurements and micromagnetic simulations.
Interferometric spectroscopy and high-speed orientation detection of individual gold nanorods
Authors: He Zhixing , Li Chengshuai , Robinson Hans D. , Zhu Yizheng
Published: January 2020

The 3D orientation and plasmon resonance wavelength in a single gold nanorod are simultaneously characterized at microsecond time scales with an interferometric, spectroscopic polarimetry of scattered light.
Synthesis and self-assembly of polyethersulfone-based amphiphilic block copolymers as microparticles for suspension immunosensors
Authors: Hu Weibin , He Xiaohong , Bai Yun , Zheng Li , Hu Yiguo , Wang Pan , Liu Xiaobo , Jia Kun
Published: January 2020

Polyethersulfone (PES) based amphiphilic block copolymer has been synthesized and transformed into immunological microparticles via confined emulsion self-assembly and surface biomodification, opens the in-vitro diagnostic application of PES.
Thickness-dependent fast wetting transitions due to the atomic layer deposition of zinc oxide on a micro-pillared surface
Authors: Duan Libing , Ji Xiangyang , Yang Yajie , Yang Sihang , Lv Xinjun , Xie Yanbo
Published: January 2020

Smart surfaces promote the fundamental understanding of wetting and are widely used in practical applications for energy and water collection.
Isolation of singlet carbene derived 2-phospha-1,3-butadienes and their sequential one-electron oxidation to radical cations and dications
Authors: Sharma Mahendra K. , Blomeyer Sebastian , Glodde Timo , Neumann Beate , Stammler Hans-Georg , Hinz Alexander , van Gastel Maurice , Ghadwal Rajendra S.
Published: January 2020

The 2-phospha-1,3-butadiene derivatives 3 are readily accessible by reduction of 2 with Mg. Sequential one-electron oxidation of 3 with GaCl3 leads to the formation of radical cations 4 and dications 5 as crystalline solids.
Correction: O2-3-Aminopropyl diazeniumdiolates suppress the progression of highly metastatic triple-negative breast cancer by inhibition of microvesicle formation via nitric oxide-based epigenetic regulation
Authors: Kang Fenghua , Zhu Jiayi , Wu Jianbing , Lv Tian , Xiang Hua , Tian Jide , Zhang Yihua , Huang Zhangjian
Published: January 2020

Correction for ‘O2-3-Aminopropyl diazeniumdiolates suppress the progression of highly metastatic triple-negative breast cancer by inhibition of microvesicle formation via nitric oxide-based epigenetic regulation’ by Fenghua Kang et al., Chem. Sci., 2018, 9, 6893–6898.
Low electronegativity Mn bulk doping intensifies charge storage of Ni2P redox shuttle for membrane-free water electrolysis
Authors: Liu Zichen , Zhang Gong , Zhang Kai , Lan Huachun , Liu Huijuan , Qu Jiuhui
Published: January 2020

A high efficiency electrochemical energy storage and high areal capacitance solid-state mediator can act as an energy vector to solve the key problem in mixtures of H2 and O2 in electrically driven water splitting architectures.
Fluorescent conjugated mesoporous polymers with N,N-diethylpropylamine for the efficient capture and real-time detection of volatile iodine
Authors: Xu Meiyun , Wang Tao , Zhou Lei , Hua Daoben
Published: January 2020

A strategy for the efficient capture and real-time detection of volatile iodine is realized by a fluorescent conjugated microporous polymer with flexible binding sites.
Black BiVO4: size tailored synthesis, rich oxygen vacancies, and sodium storage performance
Authors: Xu Xiaosa , Xu Youxun , Xu Fei , Jiang Guangshen , Jian Jie , Yu Huiwu , Zhang Enming , Shchukin Dmitry , Kaskel Stefan , Wang Hongqiang
Published: January 2020

Size tailored black BiVO4 colloids with rich oxygen vacancies were generated via facile laser processing for high performance sodium storage.
Fabrication of 3D plotted scaffold with microporous strands for bone tissue engineering
Authors: Seok Ji Min , Rajangam Thanavel , Jeong Jae Eun , Cheong Sinyoung , Joo Sang Min , Oh Seung Ja , Shin Heungsoo , Kim Sang-Heon , Park Su A
Published: January 2020

Scaffold porosity has played a key role in bone tissue engineering aimed at effective tissue regeneration, by promoting cell attachment, proliferation, and osteogenic differentiation for new bone formation.
Conjugated polymers as nanoparticle probes for fluorescence and photoacoustic imaging
Authors: Abelha Thais Fedatto , Dreiss Cécile A. , Green Mark A. , Dailey Lea Ann
Published: January 2020

In this review, the role of conjugated polymer nanoparticles (CPNs) in emerging bioimaging techniques is described.
A bis(diketopyrrolopyrrole) dimer-containing ligand in platinum(ii) polyyne oligomers exhibiting ultrafast photoinduced electron transfer with PCBM and solar cell properties
Authors: Nos Mélodie , Marineau-Plante Gabriel , Gao Di , Durandetti Muriel , Hardouin Julie , Karsenti Paul-Ludovic , Gupta Gaurav , Sharma Ganesh D. , Harvey Pierre D. , Lemouchi Cyprien , Le Pluart Loïc
Published: January 2020

We report the synthesis and characterization of a bis(diketopyrrolopyrrole) dimer-containing ligand in platinum(ii) polyyne oligomer P4 with very good photovoltaic performance, PCE = 9.54% (P4; Jsc = 16.24 mA cm−2, Voc = 0.89 V, FF = 0.66).
Protonation-induced dual fluorescence of a blue fluorescent material with twisted A–π–D–π–A configuration
Authors: Yang Jingjing , Liu Xing , Liu Zemei , Wang Long , Sun Jing , Guo Zhen , Xu Huixia , Wang Hua , Zhao Bo , Xie Guohua
Published: January 2020

Dual emission of a blue fluorescent material and its application in WOLEDs and as a fluorescent probe are investigated.
Breakthrough in high ON-state current based on Ag–GeTe8 selectors
Authors: Zhang Sifan , Wu Liangcai , Song Zhitang , Li Tao , Chen Xin , Yan Shuai , Zhu Min
Published: January 2020

A novel selector exhibits a high drive current of 10 mA by combining high-mobility Ag with highly defective GeTe8.
Detection methods for influenza A H1N1 virus with special reference to biosensors: a review
Authors: Ravina  , Dalal Anita , Mohan Hari , Prasad Minakshi , Pundir C.S.
Published: January 2020

H1N1 (Swine flu) is caused by influenza A virus, which is a member of Orthomyxoviridae family. Transmission of H1N1 occurs from human to human through air or sometimes from pigs to humans. The influenza virus has different RNA segments, which can reassert to make new virus strain with the possibility to create an outbreak in unimmunized people. Gene reassortment is a process through which new strains are emerging in pigs, as it has specific receptors for both human influenza and avian influenza viruses. H1N1 binds specifically with an α-2,6 glycosidic bond, which is present in human respiratory tract cells as well as in pigs. Considering the fact of fast multiplication of viruses inside the living cells, rapid detection methods need an hour. Currently, WHO recommended methods for the detection of swine flu include real-time PCR in specific testing centres that take 3–4 h. More recently, a number of methods such as Antigen–Antibody or RT-LAMP and DNA biosensors have also been developed that are rapid and more sensitive. This review describes the various challenges in the diagnosis of H1N1, and merits and demerits of conventional vis-à-vis latest methods with special emphasis on biosensors.
Les anticorps monoclonaux en neurologie
Authors: Blasco Hélène , Pradat Pierre-François
Published: January 2020

L’utilisation des anticorps thérapeutiques commence à se développer avec succès en neurologie. Leur efficacité est conditionnée par la capacité à contourner la principale limite à l’utilisation de ces molécules dans ce type d’indication qu’est l’accessibilité au cerveau. Le caractère multifactoriel de ces pathologies neurologiques rend également complexe l’identification d’une cible spécifique. Nous exposons dans cette revue les effets neuroprotecteurs des anticorps monoclonaux et résumons leurs activités sur les mécanismes neurodégénératifs et inflammatoires. Les anticorps monoclonaux, tels que le natalizumab, représentent une avancée importante dans le traitement de la sclérose en plaque (SEP) et sont désormais utilisés en routine. Ce type de thérapeutique est toujours en développement dans les maladies neurodégénératives, en agissant principalement sur l’agrégation des protéines mal repliées, telles que la protéine béta-amyloïde et la protéine tau. Une autre stratégie en développement consiste à bloquer les inhibiteurs physiologiques de croissance axonale et de myélinisation, tels que Nogo-A et son co-transporteur LINGO-1. L’autorisation récente par la food and drug administration américaine des anticorps monoclonaux anti-calcitonin gene-related peptide (CGRP) dans la migraine a soulevé un regain d’intérêt pour ces thérapeutiques en neurologie. La recherche est de ce fait très active pour améliorer les formes galéniques et les voies d’administration et pour étendre ces thérapeutiques à d’autres cibles.
Anticorps monoclonaux thérapeutiques
Authors: Du-Thanh Aurélie , Guillot Bernard
Published: January 2020

Les anticorps monoclonaux thérapeutiques en dermatologie ont d’abord été utilisés pour la prise en charge du psoriasis, puis, plus récemment, de l’urticaire chronique spontanée et de la dermatite atopique. Trois exemples seront abordés dans cette revue: le dupilumab, l’omalizumab et le lanadélumab. Leur mode d’action spécifique résulte d’une meilleure compréhension des mécanismes inflammatoires de ces maladies chroniques qui partageaient autrefois des traitements topiques ou systémiques plus ou moins ciblés. Leur utilisation onéreuse doit cependant être raisonnée par la mise en perspective de l’épidémiologie, de l’évaluation précise de la sévérité, de l’optimisation des traitements de première ligne, et de la balance bénéfices/risques.
Dénominations et indications des anticorps face à la réglementation et aux pratiques des laboratoires pharmaceutiques
Authors: Foucault-Fruchard Laura , Watier Hervé , Antier Daniel
Published: January 2020

Les dénominations et les indications des anticorps monoclonaux thérapeutiques doivent répondre aux réglementations en vigueur, mais s’accompagnent du développement de stratégies commerciales autour des noms commerciaux. Certaines de ces pratiques obéissent à des considérations territoriales ou juridiques, tandis que d’autres sont motivées par de réelles préoccupations médicales. Enfin, certaines ont un impact financier non négligeable pour la collectivité.
Anticorps monoclonaux biosimilaires
Authors: Beck Alain , Guillarme Davy , Fleury-Souverain Sandrine , Bodier-Montagutelli Elsa , Respaud Renaud
Published: January 2020

La mise sur le marché de biosimilaires requiert une démonstration stricte de la similarité avec l’anticorps de référence, au travers d’études précliniques et cliniques. Cet article synthétise l’ensemble des analyses physicochimiques et fonctionnelles mises en œuvre in vitro, préalables à la réalisation d’études cliniques. Pour chaque caractéristique critique de l’anticorps, nous avons détaillé les techniques analytiques communément employées, leur principe de fonctionnement, ainsi que le type d’informations que ces techniques permettent d’obtenir.
La bioproduction des anticorps monoclonaux
Authors: Joubert Simon , Dodelet Vincent , Béliard Roland , Durocher Yves
Published: January 2020

Les anticorps monoclonaux font désormais partie intégrante de l’arsenal thérapeutique pour une multitude de maladies. Étant donné le nombre croissant de produits commerciaux et de candidats en développement, l’état actuel des systèmes de bioproduction est une préoccupation majeure de l’industrie. La productivité des plateformes d’expression, ainsi que la performance des technologies utilisées dans les procédés en amont et en aval, sont des facteurs critiques qui ont un impact sur le coût et la durée du développement des anticorps thérapeutiques. De multiples stratégies de génie cellulaire peuvent être utilisées pour l’amélioration de la production des anticorps et pour un contrôle accru des modifications post-traductionnelles, comme la glycosylation, particulièrement importante, car elle peut avoir un effet prononcé sur l’activité thérapeutique des anticorps. Les avancées des techniques « omiques » rendent maintenant possibles de nouvelles approches pour l’amélioration de ces plateformes. Nous passons ici en revue les progrès en bioproduction essentiels à la croissance continue du marché des anticorps thérapeutiques.
A Novel Splice-Site Variant in SLC12A6 Causes Andermann Syndrome without Agenesis of the Corpus Callosum
Authors: Al Shibli Naema , Al-Maawali Almundher , Elmanzalawy Alaa , Al-Nabhani Maryam , Koul Roshan , Gabr Ahlam , Al Murshedi Fathiya
Published: January 2020

Andermann syndrome, otherwise known as agenesis of the corpus callosum with peripheral neuropathy (ACCPN), is an autosomal recessive motor and sensory neuropathy known to be associated with ACC and mild-to-moderate intellectual disability. We present a 7-year-old girl with infantile-onset hypotonia, mild intellectual disability, and severe motor and sensory demyelinating peripheral neuropathy. Brain magnetic resonance imaging showed intact corpus callosum. Whole exome sequencing showed a novel splice-site pathogenic variant in the SLC12A6 gene. We confirm that ACC is not a mandatory feature and suggest that the term ACCPN may be misleading.
Bilateral Hidden Isokinetic Quadriceps Performance before and after MPFL Reconstruction in Pediatric Patients
Authors: Małecki Krzysztof , Niedzielski Kryspin , Flont Paweł , Fabis-Strobin Anna , Fabis Jarosław
Published: January 2020

We perform prospective study to evaluate the isokinetic performance of quadriceps before and 1 year after medial patella-femoral ligament (MPFL) reconstruction with the adductor magnus tendon in the case of recurrent patellar dislocation. The present study is the first to describe the isokinetic function of the quadriceps of the involved and uninvolved extremity, in such a wide range. The MPFL is a crucial passive stabilizer of patella and, along with the conditions of the anatomical shape of the femoral-patellar joint and the function of quadriceps, influences the overall patellar stability. However, only a few studies have examined indirectly or directly the function of quadriceps. A total of 27 patients (average age at surgery was 15.8 years) with recurrent monolateral patellar dislocation were treated with MPFL reconstruction using the adductor magnus tendon. In the study group, healthy and operated quadriceps were evaluated for the following parameters at the velocities of 60 and 180 deg/s before surgery and in the follow-up examination: peak torque, peak torque to body weight, time to peak torque, peak torque angle, torque in 30 degree of the knee flexion (TQ 30 degree), and the torque in the first 180 milliseconds (TQ 180). Preoperative patellar instability and its normalization after MPFL reconstruction have no impact on the isokinetic quadriceps index value which depends on the time and degree of inactivity as well as implementation of appropriate physiotherapy. The increase in the quadriceps muscle strength of a healthy limb is responsible for the persistence of muscle isokinetic imbalance after MPFL reconstruction in pediatric patients. This is a level 2b study.
Use of a Mandatory Clinical Decision Unit Reduces Readmission Rates following Total Joint Arthroplasty
Authors: Manoli Arthur , Markel Jacob F. , Pizzimenti Natalie M. , Markel David C.
Published: January 2020

Readmission penalties have encouraged the implementation of protocols to reduce readmission rates. We hypothesized that by keeping postoperative patients, who return to the emergency department (ED) in a clinical decision unit (CDU) until being evaluated by the orthopaedic team, there would be a reduction in the readmission rate after total joint arthroplasty (TJA) at our institution. Our institution mandated the use of the CDU for all potential orthopaedic TJA readmissions. A retrospective review of prospectively collected data was performed on 365 patients who presented to the ED after either total hip arthroplasty (THA) or total knee arthroplasty (TKA). Patients presenting in the year prior to the implementation of the CDU program were compared with patients presenting in the year after implementation. Demographics, length of stay, comorbidities, and 30-day readmission rates were recorded. Additionally, a financial analysis was performed. Overall, for THA and TKA, there were a combined 141 ED visits prior to the implementation of the CDU program and 224 afterward; of these, 40 were readmitted before the CDU program and only 13 were readmitted afterward (p < 0.01). The financial analysis found that the overall 90-day cost for patients in the postoperative period was nearly $800 lower on average (p = 0.027) post-CDU implementation.

During the first year of the CDU project at our institution, we significantly reduced the readmission rates following TJA and demonstrated significant cost saving. This is a Level III, prognostic study.
Michigan Ophthalmology Pipeline: Exploring a Mentorship Model to Increase Diversity in Ophthalmology
Authors: Shaner Mason A. , Kaplan Ariane , Sesi Christopher , Manjunathan Abhishek , Lacy Gabrielle D. , Prisk Lauren , Crear Jara , Sugar Alan , Elam Angela , Mian Shahzad I.
Published: January 2020

Background Ethnic concordance between physicians and patients improves compliance and therapeutic benefit. Current literature shows a lack of diversity within ophthalmology. Thus, we aimed to develop a longitudinal mentorship program between first year ophthalmology residents (PGY2s) and first year medical students (M1s) coming from minority communities underrepresented in medicine (URM) to provide early exposure to the field.

Methods M1 members of URM organizations were recruited and paired with PGY2 ophthalmology residents in a mentorship program between 2017 and 2019. All participants were surveyed twice annually.

Results All 2017 M1s stated increased interest in ophthalmology, felt “satisfied” or “very satisfied” with the program, and completed all requirements. At the year-end, the mean educational value of the program for 2017 M1s was rated 4.33/5, and interest in ophthalmology 4.67/5. Quality of the clinical experiences for 2017 PGY2s 3.5/5, and the overall effectiveness of the program 3.5/5. At the year-end, the average educational value of the program for 2018 M1s was 4.4/5, and interest in ophthalmology 4.0/5. Quality of the clinical experiences for 2018 PGY2s was 3.1/5, and the overall effectiveness of the program was 3.4/5.

Conclusion Our “pipeline” program represents an ongoing effort to increase URM interest in ophthalmology. Continued assessment to identify areas for growth and improvement can optimize the program to aid other programs in initiating efforts to tackle this important issue.
Posterior Cruciate Ligament Reconstruction in the Multiple Ligament Injured Knee
Authors: Kew Michelle E. , Miller Mark D.
Published: January 2020

Multiligamentous knee injuries are challenging to treat and diagnose. Posterior cruciate ligament (PCL) injuries are commonly found in the constellation of injuries included in a multiligamentous knee injury and are caused by a posteriorly directed force on the proximal tibia with relation to the femoral condyles. A thorough history and physical examination should be performed to evaluate for associated neurovascular injuries and associated ligamentous, chondral, or bony injuries. Nonsurgical management is reserved for patients who are critically ill or have very low activity demands. Surgical reconstruction is recommended for most patients with multiligamentous knee injuries. The PCL reconstruction can be undertaken with several different graft options and reconstruction techniques, including the transtibial, arthroscopic tibial inlay, and open tibial inlay approach. The literature has a paucity of data regarding outcomes among the various reconstructive options, so the optimal surgical technique has not been established.
The 90-day Readmission Rate after Single-Bundle ACL Reconstruction Plus LET: Analysis of 2,559 Consecutive Cases from a Single Institution
Authors: Grassi Alberto , Costa Giuseppe Gianluca , Cialdella Sergio , Lo Presti Mirco , Neri Maria Pia , Zaffagnini Stefano
Published: January 2020

The aim of the present study is to examine the readmission rate within 90 days of a cohort of patients, who underwent an arthroscopic anterior cruciate ligament (ACL) reconstruction with a lateral extraarticular tenodesis, from a single highly specialized sports traumatology department. From our institutional database, we identified 2,559 patients (78.5% males and 21.5% females, with a mean age at surgery of 30.9 ± 11.5 years) who underwent primary ACL reconstruction with the same “over-the-top” technique plus lateral extraarticular tenodesis (LET) from January 2010 to December 2017. From this count, we extracted all patients who were readmitted within 90 days and focused on causes of readmission and reoperation rate. Moreover, a multivariate logistic regression was performed to identify possible variables, such as gender, age, and concomitant meniscus surgery, which could predict the risk of early readmission. From the aforementioned cohort, 58 patients (2.27%) were readmitted within 90 days from surgery after a mean time of 31 ± 23 days. The most common cause of readmission was fever and knee swelling (0.78%), followed by superficial infection (0.63%), deep infection (0.55%), and joint stiffness (0.23%). The patients' age and meniscal lesions requiring concomitant arthroscopic treatment were found to be correlated to an increased risk of early readmission. Superficial infections were more common in female patients (odds ratio [OR] = 3.01), whereas the meniscal treatment was also a significant risk factor specifically for deep infections (OR = 3.56). In conclusion, this technique of arthroscopic ACL reconstruction with LET showed a low readmission rate within 90 days from surgery. However, patients and physicians should be aware of the risk of serious complications, such as deep and superficial infections, mostly in female patients and in cases of concurrent meniscal treatments. This is a Level IV, retrospective case series study.
An automatic estimation of the ridge parameter for extreme learning machine
Authors: Naik Shraddha M. , Jagannath Ravi Prasad K. , Kuppili Venkatanareshbabu
Published: January 2020

Extreme learning machine (ELM) is an emerging learning method with a single-hidden layer feed-forward neural network that involves obtaining a solution to the system of linear equations. Unlike traditional gradient-based back-propagating neural networks, ELM is computationally efficient with fast training speed and good generalization capability. However, most of the time when applied to real-time problems, the linear system becomes ill-posed in the structure and needs the inclusion of a ridge parameter to obtain a reliable solution, and hence, the selection of the ridge parameter (C) is a crucial task. The ridge parameter is chosen heuristically from a predefined set. The generalized cross-validation is a widely used technique for the automatic estimation of the same, which is computationally expensive as it involves inversion of large matrices. The focus of the proposed work is on pragmatic aspects of the time-efficient automatic estimation of ridge parameter that result in a better generalization performance. In this work, methods are proposed that use the L-curve and U-curve techniques to automatically estimate the ridge parameter, and these methods are effective in the estimation of the ridge parameter even for systems with larger data. Through extensive numerical results, it is shown that the proposed methods outperform the existing ones in terms of accuracy, precision, sensitivity, specificity, F1-score, and computational time on various benchmark binary as well as multiclass classification data sets. Finally, the proposed methods are statistically analyzed using the nonparametric Friedman ranking test, which is also proving the effectiveness of the proposed method as it is providing a better rank for the same over existing methods.
Electronic properties of dilute-As InGaNAs alloys: A first-principles study
Authors: Borovac Damir , Sun Wei , Tan Chee-Keong , Tansu Nelson
Published: January 2020

The dilute-As InyGa1 − yN1 − xAsx alloys are explored by performing first-principles density functional theory (DFT) calculations, where the In-content is varied from 0% up to 18.75% and the As-content is varied from 0% up to 3.13%. The obtained band structures indicate a direct bandgap semiconductor, whose electronic properties are modified significantly by the addition of As-atoms into the InGaN-based system. The lattice parameters (a and c) are analyzed, and the bowing parameters of the dilute-As InGaNAs are analyzed and discussed. The electronic properties indicate the dilute-As InyGa1 − yN1 − xAsx alloys as a potential new material alternative for achieving longer wavelength emission, while utilizing a low In-content (<20%).
Extreme ultraviolet time- and angle-resolved photoemission setup with 21.5 meV resolution using high-order harmonic generation from a turn-key Yb:KGW amplifier
Authors: Liu Yangyang , Beetar John E. , Hosen Md Mofazzel , Dhakal Gyanendra , Sims Christopher , Kabir Firoza , Etienne Marc B. , Dimitri Klauss , Regmi Sabin , Liu Yong , Pathak Arjun K. , Kaczorowski Dariusz , Neupane Madhab , Chini Michael
Published: January 2020

Characterizing and controlling electronic properties of quantum materials require direct measurements of nonequilibrium electronic band structures over large regions of momentum space. Here, we demonstrate an experimental apparatus for time- and angle-resolved photoemission spectroscopy using high-order harmonic probe pulses generated by a robust, moderately high power (20 W) Yb:KGW amplifier with a tunable repetition rate between 50 and 150 kHz. By driving high-order harmonic generation (HHG) with the second harmonic of the fundamental 1025 nm laser pulses, we show that single-harmonic probe pulses at 21.8 eV photon energy can be effectively isolated without the use of a monochromator. The on-target photon flux can reach 5 × 1010 photons/s at 50 kHz, and the time resolution is measured to be 320 fs. The relatively long pulse duration of the Yb-driven HHG source allows us to reach an excellent energy resolution of 21.5 meV, which is achieved by suppressing the space-charge broadening using a low photon flux of 1.5 × 108 photons/s at a higher repetition rate of 150 kHz. The capabilities of the setup are demonstrated through measurements in the topological semimetal ZrSiS and the topological insulator Sb2−xGdxTe3.
A laminated energetic electrostatic analyzer for 0–5 keV charged particles
Authors: Maldonado C. A. , Eyler Z. , Pierce B. , Matson L. , Neal P. , Richards H. , Balthazor R. L. , Harley J. , McHarg M. G.
Published: January 2020

A compact electrostatic energy bandpass filter based on a laminated analyzer design has been developed to measure charged particle fluxes at energies ranging from 0 to 5 keV. The sensor head has been successfully tested against a low energy magnetically filtered plasma source and an ion beam source capable of producing energetic ions in the range of 100–1250 eV. Additionally, the instrument has demonstrated the ability to accurately measure negative spacecraft frame charging using a low Earth orbit plasma simulator. The effects of the spacecraft frame charging on the measured energy distribution measurements and the impact regarding the derived charged particle density and temperature parameters are also examined.