bluuebunny/crossref_metadata_2025 huggingface dataset:
Continuous Measurements of Temporal and Vertical Variations in Atmospheric CO2 and Its δ13C in and above a Subtropical Plantation
Authors: Chen Changhua , Wen Xuefa , Wang Jingyuan , Guo Qingjun
Published: May 2021

Atmospheric CO2 dynamics in forest ecosystems are dependent on interactions between photosynthesis, respiration, and turbulent mixing processes; however, the carbon isotopic composition of atmospheric CO2 (δ13C) is not well established due to limited measurement reports. In this study, a seven-inlet profile system with a Picarro analyzer was developed to conduct continuous in situ measurements of CO2 and its δ13C in and above a subtropical plantation from 2015 to 2017. Results showed that ecosystem CO2 concentration was the lowest in the afternoon and reached its peak at dawn, which mirrored variations in its δ13C in and above the canopy. Inverse seasonal variations were apparent between CO2 and its δ13C in and above the canopy, and δ13C was positive during the peak growing season and negative at other times. Diel and seasonal variations in ecosystem CO2 and its δ13C were mainly affected by the vapor pressure deficit, followed by photosynthetic active radiation, temperature, and the enhanced vegetation index in and above the canopy; however, environmental and physiological factors had reverse or no effects near the forest floor. Nocturnal gradients of vertical variations in atmospheric CO2 and its δ13C were greater than diurnal variations due to weak turbulent mixing under more stable atmospheric conditions overnight. These results implicate that photosynthesis and respiration dominated CO2 dynamics above the canopy, while CO2 recycling by photosynthesis and turbulent mixing changed CO2 dynamics in the canopy.
Electrochemical Deposition of SiO2-Coatings on a Carbon Fiber
Authors: Galyshev Sergei , Postnova Evgeniya
Published: May 2021

Research on carbon fiber oxide coatings is primarily focused on metal matrix composites. Such coatings act as a diffusion barrier between a matrix and a fiber and, in addition, they can be weak boundaries that significantly increase the mechanical properties of metal matrix composites. A simple and economical method of coating deposition is the sol-gel method. However, it does not allow for control of the thickness of the carbon fiber coating. To eliminate this limitation, a combined method is used that includes sol-gel technology and electrochemical deposition. The paper presents the results of studies on the production of SiO2 coatings on carbon fibers by the above method. The effect of current density, deposition time, salt concentration, pH of the reaction medium, TEOS/H2O molar ratio, and alcohol concentration in the reaction medium on the structure and thickness of the coatings was studied.
3D Probabilistic Modelling and Uncertainty Analysis of Glacial and Post-Glacial Deposits of the City of Saguenay, Canada
Authors: Salsabili Mohammad , Saeidi Ali , Rouleau Alain , Nastev Miroslav
Published: May 2021

Knowledge of the stratigraphic architecture and geotechnical properties of surficial soil sediments is essential for geotechnical risk assessment. In the Saguenay study area, the Quaternary deposits consist of a basal till layer and heterogeneous post-glacial deposits. Considering the stratigraphic setting and soil type heterogeneity, a multistep stochastic methodology is developed for 3D geological modelling and quantification of the associated uncertainties. This methodology is adopted for regional studies and involves geostatistical interpolation and simulation methods. Empirical Bayesian kriging (EBK) is applied to generate the bedrock topography map and determine the thickness of the till sediments and their uncertainties. The locally varying mean and variance of the EBK method enable accounting for data complexity and moderate nonstationarity. Sequential indicator simulation is then performed to determine the occurrence probability of the discontinuous post-glacial sediments (clay, sand and gravel) on top of the basal till layer. The individual thickness maps of the discontinuous soil layers and uncertainties are generated in a probabilistic manner. The proposed stochastic framework is suitable for heterogeneous soil deposits characterised with complex surface and subsurface datasets.
Subsequent Dyslipidemia and Factors Associated with Mortality in Schizophrenia: A Population-Based Study in Taiwan
Authors: Hsu Mei-Chi , Ouyang Wen-Chen
Published: May 2021

Background: Persons with schizophrenia are at greater risk of developing subsequent medical conditions. To date, few studies have examined comprehensively the risks, mortality and survival rates in schizophrenia and subsequent dyslipidemia over different time periods. The objective of this study was to evaluate the occurrence of subsequent dyslipidemia after the diagnosis of schizophrenia, and factors associated with mortality and survival rate in patients with schizophrenia. Methods: We used a population-based cohort from Taiwan National Health Insurance Research Database, to investigate in patients whom were first diagnosed with schizophrenia during the period from 1997 through 2009, the risk of subsequent dyslipidemia during follow-up. Cumulative incidences and hazard ratios after adjusting for competing mortality risks were calculated. Results: A total of 20,964 eligible patients were included. Risks (i.e., comorbidity) and protective factors (i.e., statin use) have significant impacts on mortality. The mortality exhibits a U-shaped pattern by age. After 50, the risk of death increases with age. Risk of mortality before 50 increases with a decrease in age. Risks differed by the duration time to subsequent dyslipidemia after schizophrenia. Mean duration was 63.55 months in the survive group, and 43.19 months in the deceased group. The 5-, 10-, and 15-year survival rates for patients with schizophrenia and subsequent dyslipidemia were 97.5, 90, and 79.18%, respectively. Conclusion: Early occurrence of subsequent dyslipidemia is associated with increased overall mortality in patients with schizophrenia.
Students’ Perceptions of Instructional Rubrics in Neurological Physical Therapy and Their Effects on Students’ Engagement and Course Satisfaction
Authors: García-Ros Rafael , Ruescas-Nicolau Maria-Arantzazu , Cezón-Serrano Natalia , Carrasco Juan J. , Pérez-Alenda Sofía , Sastre-Arbona Clara , San Martín-Valenzuela Constanza , Flor-Rufino Cristina , Sánchez-Sánchez Maria Luz
Published: May 2021

One of the main challenges faced by physical therapy (PT) students is to learn the practical skills involved in neurological physical therapy (PT). To help them to acquire these skills, a set of rubrics were designed for formative purposes. This paper presents the process followed in the creation of these rubrics and their application in the classroom, noting that students perceived them as valid, reliable, and highly useful for learning. The perception of the validity and usefulness of the rubrics has different closely related dimensions, showing homogeneous values across the students´ sociodemographic and educational variables, with the exception of dedication to studying, which showed a significant relationship with schoolwork engagement and course satisfaction. The adequacy of the hypothesized structural model of the relationships among the variables was confirmed. Direct effects of the perception of the rubrics’ validity and engagement on course satisfaction were found, as well as direct effects of the assessment of the usefulness of the rubrics on schoolwork engagement and indirect effects on course satisfaction through this latter variable. The results are discussed taking into account the conclusions of previous research and different instructional implications.
Stress, Anxiety, and Depression for Chinese Residents in Japan during the COVID-19 Pandemic
Authors: Hu Qin , Umeda Maki
Published: May 2021

The coronavirus disease 2019 (COVID-19) has profoundly affected the psychological well-being of foreign residents. This study examines stress, anxiety, and depression levels in Chinese residents in Japan during the COVID-19 pandemic. It identifies risk factors and the roles of disaster preparedness and social support. An online survey among Chinese residents in Japan was conducted from 22 June to 14 July 2020. The Depression, Anxiety and Stress Scale, Disaster Preparedness for Resilience Checklist, and Social Support Rate Scale were used to measure psychological symptoms. Multivariable linear regressions identified the risk factors and positive effects of disaster preparedness and social support. Of the total 497 participants, 45.3%, 66.6%, and 54.3% reported severe stress, anxiety, and depressive symptoms, respectively. People with a lower level of education, a higher level of economic influence, the presence of COVID-19 symptoms, and confirmed or suspected family or friends in China were associated with higher levels of stress, anxiety, and depression. This study, to the best of our knowledge, is the first survey to reveal the protective role of disaster preparedness in reducing psychological symptoms during the pandemic. It offers unique data for further research on how to promote the mental health of vulnerable populations including foreign residents.
Progress towards the Development of Research Agenda and the Launch of Knowledge Hub: The WHO Thematic Platform for Health Emergency and Disaster Risk Management Research Network (Health EDRM RN)
Authors: Kayano Ryoma , Nomura Shuhei , Abrahams Jonathan , Huda Qudsia , Chan Emily Y. Y. , Murray Virginia
Published: May 2021

In response to the increasing burden of recent health emergencies and disasters, the World Health Organization (WHO) and its partners established the WHO thematic platform for health emergency and disaster risk management research network (health EDRM RN) in 2016, with the purposes of promoting global research collaboration among various stakeholders and enhancing research activities that generate evidence to manage health risks associated with all types of emergencies and disasters. With the strong support and involvement of all WHO regional offices, the health EDRM RN now works with more than 200 global experts and partners to implement its purposes. The 1st and 2nd Core Group Meetings of the health EDRM RN were held on 17–18 October 2019 and 27 November 2020, respectively, to discuss the development of a global research agenda that the health EDRM RN will focus on facilitating, promoting, synthesizing and implementing, taking into account the emergence of the coronavirus disease 2019 (COVID-19) (health EDRM RN research agenda). A focus of the meetings was the establishment of an online platform to share information and knowledge, including the databases that the health EDRM RN accumulates (WHO health EDRM knowledge hub). This paper presents a summary of the discussion results of the meetings.
Evolution of the Illegal Substances Market and Substance Users’ Social Situation and Health during the COVID-19 Pandemic
Authors: Gaume Jacques , Schmutz Elodie , Daeppen Jean-Bernard , Zobel Frank
Published: May 2021

The outbreak of the COVID-19 pandemic and the measures taken for tackling it had the potential to lead to deep modifications in the supply of illegal drugs and to impact substance users’ health and social situation. To investigate this, we used mixed methods, i.e., quantitative data collected with a brief questionnaire from substance users receiving opioid agonist treatment in a treatment centre in Switzerland (N = 49), and qualitative data obtained using semi-structured phone interviews among a sub-group of participants (N = 17). We repeated data collection twice over four weeks to investigate trends over time (N = 51 and 14 at wave 2). Findings consistently showed the limited impact of the COVID-19 outbreak on the illegal substance market. Over the two waves, the supply, price and purity of three main illegal substances did not significantly vary. Substance use was estimated as usual by most, trending toward a decrease. The impact of the pandemic on participants’ social situation and health was appraised as low to medium. Nevertheless, a minority of participants reported higher impact and multivariate analyses showed a more important impact for those who were female, younger, and not using multiple substances. This process was implemented quickly and provided an understanding of the short-term impact of the pandemic on drug markets and users.
Methods for Estimating Avoidable Costs of Excessive Alcohol Consumption
Authors: Gavurova Beata , Tarhanicova Miriama
Published: May 2021

Background: Alcohol is a risk factor with serious consequences for society and individuals. This study aims to present methods and approaches that might be used to estimate the costs related to excessive alcohol consumption. It emphasizes the need for general methods and approaches that are easily applicable, because the level of digitalization and data availability vary across regions. The lack of data makes many methods inapplicable and useless. The ease of applicability will help to make cost-of-illness studies and their results comparable globally. Methods: This study is based on data from the Czech Republic in 2017. Drinking alcohol results in costs of healthcare, social care, law enforcement, and administrative costs of public authorities. To quantify the cost of drinking in the Czech Republic, the top-down approach, bottom-up approach, human capital approach and attributable fractions were used. Results: In 2017, the cost related to alcohol was estimated at 0.66% of the national GDP. Lost productivity represented 54.45% of total cost related to alcohol. All cost related to alcohol is considered to be avoidable. Conclusions: The methods and approaches applied to estimate the cost of disease or any other health issue should be generalized regarding the availability of data and specifics of provided services to people who are addicted or have any kind of disability.
Food Sources of Shortfall Nutrients among Latin Americans: Results from the Latin American Study of Health and Nutrition (ELANS)
Authors: Barco Leme Ana Carolina , Fisberg Regina Mara , Veroneze de Mello Aline , Sales Cristiane Hermes , Ferrari Gerson , Haines Jess , Rigotti Attilo , Gómez Georgina , Kovalskys Irina , Cortés Sanabria Lilia Yadira , Herrera-Cuenca Marianella , Yépez Garcia Martha Cecília , Pareja Rossina G. , Fisberg Mauro
Published: May 2021

Increased consumption of energy-dense, nutrient-poor foods can lead to inadequate intakes of shortfall nutrients, including vitamin A, D, C, and E, dietary folate, calcium, iron, magnesium, potassium, and fiber. The objective was to examine the prevalence of inadequate intake of shortfall nutrients and identify food sources of shortfall nutrients in eight Latin American countries. Data from ELANS, a multi-country, population-based study of 9218 adolescents and adults were used. Dietary intake was collected through two 24 h Recalls from participants living in urban areas of Argentina, Brazil, Chile, Colombia, Ecuador, Peru, and Venezuela. Foods and beverages were classified using the adapted version of the NHANES “What We Eat in America” system. Nutrients inadequacy was estimated using the Institute of Medicine recommendations and descriptive statistics were calculated. Prevalence of inadequacy was above 50% for most of the nutrients, which the exception of vitamin C with a prevalence of inadequacy of 39%. Milk, cheese, seafoods, breads, and fruit juices/drinks were among the top 5 sources for each of the 10 shortfall nutrients examined. Many food categories were top contributors to more than one dietary component examined. Understanding the nutrient intake and food sources can help inform dietary guidance and intervention approaches.
Seaweeds as Valuable Sources of Essential Fatty Acids for Human Nutrition
Authors: Rocha Carolina P. , Pacheco Diana , Cotas João , Marques João C. , Pereira Leonel , Gonçalves Ana M. M.
Published: May 2021

The overexploitation of terrestrial habitats, combined with the ever-growing demand for food, has led to the search for alternative food sources. The importance of seaweeds as food sources has been growing, and their potential as sources of fatty acids (FA) make seaweeds an interesting feedstock for the food and nutraceutical industries. The aim of this study is to assess the potential of five red seaweeds (Asparagospis armata, Calliblepharis jubata, Chondracanthus teedei var. lusitanicus, Gracilaria gracilis, and Grateloupia turuturu) and three brown seaweeds (Colpomenia peregrina, Sargassum muticum and Undaria pinnatifida), harvested in central Portugal, as effective sources of essential FA for food or as dietary supplements. FA were extracted from the biomass, transmethylated to methyl esters, and analyzed through gas chromatography-mass spectrometry. G. gracilis presented the highest content of saturated fatty acids (SFA) (41.49 mg·g−1), whereas C. jubata exhibited the highest content of highly unsaturated fatty acids (HUFA) (28.56 mg·g−1); the three G. turuturu life cycle stages presented prominent SFA and HUFA contents. Omega-6/omega-3 ratios were assessed and, in combination with PUFA+HUFA/SFA ratios, it is suggested that C. jubata and U. pinnatifida may be the algae with highest nutraceutical potential, promoting health benefits and contributing to a balanced dietary intake of fatty acids.
Improvement Path for Resource-Constrained Cities Identified Using an Environmental Co-Governance Assessment Framework Based on BWM-mV Model
Authors: Wang Jian , Huang Jin-Chun , Huang Shan-Lin , Tzeng Gwo-Hshiung , Zhu Ting
Published: May 2021

Global warming and extreme weather have increased most people’s awareness of the problem of environmental destruction. In the domain of sustainable development, environmental governance has received considerable scholarly attention. However, protecting and improving the environment requires not only substantial capital investment but also cooperation among stakeholders. Therefore, based on the network structure of stakeholders, the best–worst method (BWM) and modified Vlsekriterijumska Optimizacija I Kompromisno Resenje method were combined to form an environmental co-governance assessment framework that can be used to evaluate the effects of various policies and identify strategies for further improvement through data analysis (henceforth the BWM-mV model). This mechanism is not only useful for evaluating the effectiveness of environmental governance policies but also for generating suggestions to enhance these policies. Hence, the BWM-mV model is particularly suitable for local governments with limited resources in time, money, or labor. Pingxiang City Government is currently subject to such limitations and was therefore selected as the subject of an empirical case study. The results of this study revealed that the aspects (i.e., criteria) the Pingxiang City Government should urgently improve on pertain to a high-quality information communication platform (C13) and smooth joint decision-making by stakeholders (C24).
CBSI as a Social Innovation to Promote the Health of Older People in Japan
Authors: Yasumoto Saori , Gondo Yasuyuki
Published: May 2021

In this paper, we introduce a concept called community-based social innovation (CBSI). CBSI programs have been introduced to improve the physical and psychological well-being of older people. CBSI programs encourage older people to (1) take care of themselves and their peers, (2) sustain their well-being, and (3) create a cohesive and inclusive community. Although the emergence of CBSI programs is a global phenomenon, the variations, effectiveness, and sustainability of these programs are unknown. To uncover information about the Japanese version of CBSI programs, we conducted observations and face-to-face interviews with related personnel at two CBSI programs in rural areas of Japan in 2018. We found both positive and negative aspects in the current form of CBSI programs. As for the positives, these programs promote older people’s physical and psychological well-being and enhance community cohesiveness. However, CBSI programs face challenges, including that groups tend to be gender and age specific: men and the younger-old are less likely to show interest. A group-specific approach to CBSI programs could cause future community division, which would be contrary to the goal. Given the continued advancement of the aging population, a new approach to participant recruitment is needed.
Independent Risk Factors for Sepsis-Associated Cardiac Arrest in Patients with Septic Shock
Authors: Yang Won Soek , Kim Youn-Jung , Ryoo Seung Mok , Kim Won Young
Published: May 2021

The clinical characteristics and laboratory values of patients with septic shock who experience in-hospital cardiac arrest (IHCA) have not been well studied. This study aimed to evaluate the prevalence of IHCA after admission into the emergency department and to identify the factors that increase the risk of IHCA in septic shock patients. This observational cohort study used a prospective registry of septic shock patients and was conducted at the emergency department of a university-affiliated hospital. The data of 887 adult (age ≥ 18 years) septic shock (defined using the Sepsis-3 criteria) patients who were treated with a protocol-driven resuscitation bundle therapy and were admitted to the intensive care unit between January 2010 and September 2018 were analyzed. The primary endpoint was the occurrence of sepsis-associated cardiac arrest. The patient mean age was 65 years, and 61.8% were men. Sepsis-associated cardiac arrest occurred in 25.3% of patients (n = 224). The 28-day survival rate after cardiac arrest was 6.7%. Multivariate logistic regression identified chronic pulmonary disease (odds ratio (OR) 2.06), hypertension (OR 0.48), unknown infection source (OR 1.82), a hepatobiliary infection source (OR 0.25), C-reactive protein (OR 1.03), and serum lactate level 6 h from shock (OR 1.34). Considering the high mortality rate of sepsis-associated cardiac arrest after cardiopulmonary resuscitation, appropriate monitoring is required in septic shock patients with major risk factors for IHCA.
Climate Change, Health Risks, and Vulnerabilities in Burkina Faso: A Qualitative Study on the Perceptions of National Policymakers
Authors: Sorgho Raissa , Jungmann Maximilian , Souares Aurélia , Danquah Ina , Sauerborn Rainer
Published: May 2021

Climate change (CC) constitutes one of the greatest threats to human health, and requires political awareness for effective and efficient adaptation planning. This study identified the perceptions of climate change and health adaptation (CC&H) among relevant stakeholders, decision-makers, and policymakers (SDPs) in Burkina Faso (BF) by determining their perceptions of CC, of related health risks and vulnerabilities, and of CC impacts on agriculture and food security. We carried out 35 semi-structured, qualitative in-depth interviews with SDPs, representing national governmental institutions, international organizations, and civil society organizations. The interviews were analyzed using content analysis. SDPs shared similar perceptions of CC and concurred with three ideas (1) CC is a real and lived experience in BF; (2) the population is aware of climatic changes in their environment; (3) CC is intertwined with the agricultural and economic development of the country. SDPs identified biodiversity loss, floods, droughts, and extreme heat as posing the highest risk to health. They elaborated five exposure pathways that are and will be affected by CC: water quality and quantity, heat stress, food supply and safety, vector borne diseases, and air quality. In conclusion, SDPs in Burkina Faso are highly aware of CC hazards, relevant health exposure pathways, and their corresponding health outcomes. Mental health and the interplay between social factors and complex health risks constitute perception gaps. SDPs perceived CC&H risks and vulnerabilities align with current evidence.
Which Meso-Level Characteristics of Early Childhood Education and Care Centers Are Associated with Health, Health Behavior, and Well-Being of Young Children? Findings of a Scoping Review
Authors: Herr Raphael M. , Diehl Katharina , Schneider Sven , Osenbruegge Nina , Memmer Nicole , Sachse Steffi , Hoffmann Stephanie , Wachtler Benjamin , Herke Max , Pischke Claudia R. , Novelli Anna , Hilger-Kolb Jennifer
Published: May 2021

Characteristics of early childhood education and care (ECEC) centers might be relevant for children’s health. This scoping review aims to provide an overview of the association between meso-level characteristics (MLCs) of ECEC centers with children’s health, health behavior, and wellbeing. Five databases were searched for quantitative and qualitative research articles published in English or German since 1 January 2000 on health, health behavior, and wellbeing of children aged 0 to 6 years considering MLCs of ECEC centers. Two authors screened 10,396 potentially eligible manuscripts and identified 117 papers, including 3077 examinations of the association between MLCs and children’s health indicators (Kappas > 0.91). Five categories of MLCs were identified: (1) structural characteristics, (2) equipment/furnishings, (3) location, (4) facilities/environment, (5) culture/activities/policies/practices, and 6) staff. Only very few studies found an association of MLCs with body weight/obesity, and general health and wellbeing. Especially physical activity and mental health were related to MLCs. In general, the location (rural vs. urban, neighborhood status) seemed to be a relevant health aspect. MLCs of ECEC centers appeared relevant for child health indicators to different degrees. Future research should focus on these associations, in detail, to identify concrete ECEC indicators that can support health promotion in early childhood.
Patterns of Agricultural Diversification in China and Its Policy Implications for Agricultural Modernization
Authors: Han Hongyun , Lin Hui
Published: May 2021

Based on the value of agricultural farm products produced by different subsectors in China, the foregoing analysis reveals the dynamic character of agricultural diversification by which, this study seeks to examine the evolutionary process of Chinese agriculture through a quantitative study of agricultural diversification at both national and provincial levels. In the initial stages of reform and opening up, the degree of agricultural diversification in the southwest was relatively high; then the center of agricultural diversification gradually moved to the southeast of China; finally, the degree of agricultural diversification in the economically developed eastern provinces was obviously higher than those in other regions in 2019. It was seen that some provinces in the eastern and central south regions moved toward increasing diversification in one direction, and other provinces changed direction, first moving toward diversification and later toward concentration or vice versa. These oscillations implied that there was a cyclic tendency of agricultural diversification along with an increase in per capita GDP. Generally speaking, the patterns of diversification differed across regions due to diversified agricultural subsectors resulting from different natural and socio-economic circumstances. In particular, in less developed regions with lower agricultural diversification levels, farming agriculture persistently dominated the leading position with relatively more resistance to modernizing trends in other aspects of agriculture. It is urgent for the Chinese government to figure out ways off reconciling agricultural productivity with environmental quality through the ecological intensification of agriculture.
Expected Labor Market Affiliation: A New Method Illustrated by Estimating the Impact of Perceived Stress on Time in Work, Sickness Absence and Unemployment of 37,605 Danish Employees
Authors: Pedersen Jacob , Solovieva Svetlana , Thorsen Sannie Vester , Andersen Malene Friis , Bültmann Ute
Published: May 2021

As detailed data on labor market affiliation become more accessible, new approaches are needed to address the complex patterns of labor market affiliation. We introduce the expected labor market affiliation (ELMA) method by estimating the time-restricted impact of perceived stress on labor market affiliation in a large sample of Danish employees. Data from two national surveys were linked with a national register. A multi-state proportional hazards model was used to calculate ELMA estimates, i.e., the number of days in work, sickness absence, and unemployment during a 4-year follow-up period, stratified by gender and age. Among employees reporting frequent work-related stress, the expected number of working days decreased with age, ranging from 103 days lost among older women to 37 days lost among younger and middle-aged men. Young and middle-aged women reporting frequent work- and personal life-related stress lost 62 and 81 working days, respectively, and had more days of sickness absence (34 days and 42 days). In conclusion, we showed that perceived stress affects the labor market affiliation. The ELMA estimates provide a detailed understanding of the impact of perceived stress on labor market affiliation over time, and may inform policy and practice towards a more healthy and sustainable working life.
Body Composition in Elite Soccer Players from Youth to Senior Squad
Authors: Spehnjak Marijan , Gušić Marko , Molnar Slavko , Baić Mario , Andrašić Slobodan , Selimi Musa , Mačak Draženka , Madić Dejan M. , Žilič Fišer Suzana , Sporiš Goran , Trajković Nebojša
Published: May 2021

There is a strong relationship between body composition and performance in male soccer players. This study aimed to display an optimal body height and weight, and body composition profile of male soccer players for four competitive age groups. This cross-sectional study included four groups: U-15 (n = 152), U-17 (n = 154), U-19 (n = 61), and seniors (n = 27). Body height and weight were measured under standard conditions, and the bioelectrical impedance analyzer (BIA) analyzed body composition. On average, soccer players in the U-15 group had significantly lower body height, weight, body mass index, skeletal muscle mass, fat-free mass, total body water and basal metabolic rate than U-17, U-19 and seniors, but a higher percentage of body fat than U-17 and U-19, p < 0.05. In addition, the results show significant non-linear increases in body height, weight and body composition as the age of soccer players increases, with the exception of the percentage of body fat mass, which tends to significantly decrease with age. The main findings of this study are that body fat mass remains stable and similar across all age groups, including in the senior squad.
Effect of Grandmaternal Smoking on Body Size and Proportions at Birth
Authors: Rumrich Isabell Katharina , Hänninen Otto , Viluksela Matti , Vähäkangas Kirsi
Published: May 2021

Many long-term adverse effects of smoking during pregnancy are known. Increasingly, adverse effects in the grandchild after grandmaternal smoking during pregnancy are reported. We explored this in a birth cohort of 24,000 grandmother–mother–child triads identified from the Finnish Medical Birth Register in 1991–2016. Multiple logistic regression was used to analyze the association between any smoking during pregnancy by both grandmother and mother, or only grandmother or mother on adverse birth outcomes. No smoking by neither grandmother nor mother was used as the reference. As endpoints, preterm birth, low birth weight, small for gestational age (birth weight, birth length, head circumference), and body proportionality (low ponderal index, high brain-to-body ratio, high head-to-length ratio) were included. Smoking by both grandmother and mother was consistently associated with higher risks than smoking only by the mother. Birth length and weight were especially sensitive to (grand)maternal smoking. In conclusion, the combined effect of grandmaternal and maternal smoking is associated with higher risks than only maternal smoking.
Start-Up of Chitosan-Assisted Anaerobic Sludge Bed Reactors Treating Light Oxygenated Solvents under Intermittent Operation
Authors: Torres Keisy , Álvarez-Hornos Francisco Javier , Gabaldón Carmen , Marzal Paula
Published: May 2021

Quality of the granular sludge developed during the start-up of anaerobic up-flow sludge bed reactors is of crucial importance to ensure the process feasibility of treating industrial wastewater such as those containing solvents. In this study, the microbial granule formation from suspended-growth biomass was investigated in two chitosan-assisted reactors. These reactors operated mimicking industrial sites working with night closures treating a mixture of ethanol, ethyl acetate, and 1-ethoxy-2-propanol. Each reactor operated under different hydrodynamic regimes typical from UASB (R1: <0.15 m h−1) and EGSB (R2: 3 m h−1). High soluble COD removal efficiencies (>90%) accompanied by rapid formation of robust anaerobic granules were achieved at both up-flow velocity levels. After three weeks from the start-up, mean size diameters of 475 µm and 354 µm were achieved for R1 and R2, respectively. The performance of the process was found to be stable for the whole operational period of 106 days treating intermittent OLR up to 13 kg COD m−3 d−1. A memory dose of chitosan at day 42 was beneficial to guarantee good quality of the granules by offsetting the negative impact of intermittent water supply on the granular size. Methanocorpusculum was identified as the dominant archaea at both up-flow velocities. Acetobacterium, Geobacter and Desulfovibrio bacteria were also abundant, demonstrating its role on the degradation of light-oxygenated solvents.
A Systematic Review of Oropharyngeal Dysphagia Models in Rodents
Authors: Kim Han-Na , Kim Ji-Youn
Published: May 2021

Oropharyngeal dysphagia is a condition characterized by swallowing difficulty in the mouth and pharynx, which can be due to various factors. Animal models of oropharyngeal dysphagia are essential to confirm the cause-specific symptoms, pathological findings, and the effect of treatment. Recently, various animal models of dysphagia have been reported. The purpose of this review is to organize the rodent models of oropharyngeal dysphagia reported to date. The articles were obtained from Medline, Embase, and the Cochrane library, and selected following the PRISMA guideline. The animal models in which oropharyngeal dysphagia was induced in rats or mice were selected and classified based on the diseases causing oropharyngeal dysphagia. The animal used, method of inducing dysphagia, and screening methods and results were collected from the selected 37 articles. Various rodent models of oropharyngeal dysphagia provide distinctive information on atypical swallowing. Applying and analyzing the treatment in rodent models of dysphagia induced from various causes is an essential process to develop symptom-specific treatments. Therefore, the results of this study provide fundamental and important data for selecting appropriate animal models to study dysphagia.
Representative Bacillus sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation
Authors: López-Moreno Ana , Torres-Sánchez Alfonso , Acuña Inmaculada , Suárez Antonio , Aguilera Margarita
Published: May 2021

Human gut microbiota harbors numerous microbial species with molecular enzymatic potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species isolation and description of their specific molecular features remain largely unexplored. In the present study, we focused on the cultivation and selection of species able to tolerate or biodegrade the endocrine disruptor bisphenol A (BPA), a xenobiotic extensively found in food plastic containers. Chemical xenobiotic addition methods for the directed isolation, culturing, Whole Genome Sequencing (WGS), phylogenomic identification, and specific gene-encoding searches have been applied to isolate microorganisms, assess their BPA metabolization potential, and describe encoded catabolic pathways. BPA-tolerant strains were isolated from 30% of infant fecal microbial culture libraries analyzed. Most isolated strains were phylogenetically related to the operational taxonomic group Bacillus amyloliquefaciens spp. Importantly, WGS analysis of microbial representative strain, Bacillus sp. AM1 identified the four complete molecular pathways involved on BPA degradation indicating its versatility and high potential to degrade BPA. Pathways for Exopolysaccharide (EPS) and Polyhydroxyalkanates (PHA) biopolymer synthesis were also identified and phenotypically confirmed by transmission electronic microscopy (TEM). These microbial biopolymers could generally contribute to capture and/or deposit xenobiotics.
The Endocannabinoid System in the Mediterranean Mussel Mytilus galloprovincialis: Possible Mediators of the Immune Activity?
Authors: Mosca Francesco , Zarivi Osvaldo , Battista Natalia , Maccarrone Mauro , Tiscar Pietro Giorgio
Published: May 2021

Anandamide (AEA) is one of the best characterized members of the endocannabinoid family and its involvement in many pathophysiological processes has been well documented in vertebrates and invertebrates. Here, we report the biochemical and functional characterization of key elements of the endocannabinoid system in hemocytes isolated from the Mediterranean mussel Mytilus galloprovincialis. We also show the effects of exogenous AEA, as well as of capsaicin, on the cell ability to migrate and to activate the respiratory burst, upon in vitro stimulation of phagocytosis. Interestingly, our findings show that both AEA and capsaicin suppress the hemocyte response and that the use of selective antagonists of CB2 and TRPV1 receptors revert their inhibitory effects. Overall, present data support previous evidence on the presence of endocannabinoid signaling in mollusks and advance our knowledge about the evolutionary origins of this endogenous system and its role in the innate response of mollusks.
Inhibitors of Chemoresistance Pathways in Combination with Ara-C to Overcome Multidrug Resistance in AML. A Mini Review
Authors: Fajardo-Orduña Guadalupe Rosario , Ledesma-Martínez Edgar , Aguiñiga-Sánchez Itzen , Mora-García María de Lourdes , Weiss-Steider Benny , Santiago-Osorio Edelmiro
Published: May 2021

Acute myeloid leukemia (AML), the most common type of leukemia in older adults, is a heterogeneous disease that originates from the clonal expansion of undifferentiated hematopoietic progenitor cells. These cells present a remarkable variety of genes and proteins with altered expression and function. Despite significant advances in understanding the molecular panorama of AML and the development of therapies that target mutations, survival has not improved significantly, and the therapy standard is still based on highly toxic chemotherapy, which includes cytarabine (Ara-C) and allogeneic hematopoietic cell transplantation. Approximately 60% of AML patients respond favorably to these treatments and go into complete remission; however, most eventually relapse, develop refractory disease or chemoresistance, and do not survive for more than five years. Therefore, drug resistance that initially occurs in leukemic cells (primary resistance) or that develops during or after treatment (acquired resistance) has become the main obstacle to AML treatment. In this work, the main molecules responsible for generating chemoresistance to Ara-C in AML are discussed, as well as some of the newer strategies to overcome it, such as the inclusion of molecules that can induce synergistic cytotoxicity with Ara-C (MNKI-8e, emodin, metformin and niclosamide), subtoxic concentrations of chemotherapy (PD0332991), and potently antineoplastic treatments that do not damage nonmalignant cells (heteronemin or hydroxyurea + azidothymidine).
Epigenetic Regulation of Neuroinflammation in Parkinson’s Disease
Authors: Rasheed Madiha , Liang Junhan , Wang Chaolei , Deng Yulin , Chen Zixuan
Published: May 2021

Neuroinflammation is one of the most significant factors involved in the initiation and progression of Parkinson’s disease. PD is a neurodegenerative disorder with a motor disability linked with various complex and diversified risk factors. These factors trigger myriads of cellular and molecular processes, such as misfolding defective proteins, oxidative stress, mitochondrial dysfunction, and neurotoxic substances that induce selective neurodegeneration of dopamine neurons. This neuronal damage activates the neuronal immune system, including glial cells and inflammatory cytokines, to trigger neuroinflammation. The transition of acute to chronic neuroinflammation enhances the susceptibility of inflammation-induced dopaminergic neuron damage, forming a vicious cycle and prompting an individual to PD development. Epigenetic mechanisms recently have been at the forefront of the regulation of neuroinflammatory factors in PD, proposing a new dawn for breaking this vicious cycle. This review examined the core epigenetic mechanisms involved in the activation and phenotypic transformation of glial cells mediated neuroinflammation in PD. We found that epigenetic mechanisms do not work independently, despite being coordinated with each other to activate neuroinflammatory pathways. In this regard, we attempted to find the synergic correlation and contribution of these epigenetic modifications with various neuroinflammatory pathways to broaden the canvas of underlying pathological mechanisms involved in PD development. Moreover, this study highlighted the dual characteristics (neuroprotective/neurotoxic) of these epigenetic marks, which may counteract PD pathogenesis and make them potential candidates for devising future PD diagnosis and treatment.
The Contribution of Small Vessel Disease to Neurodegeneration: Focus on Alzheimer’s Disease, Parkinson’s Disease and Multiple Sclerosis
Authors: Paolini Paoletti Federico , Simoni Simone , Parnetti Lucilla , Gaetani Lorenzo
Published: May 2021

Brain small vessel disease (SVD) refers to a variety of structural and functional changes affecting small arteries and micro vessels, and manifesting as white matter changes, microbleeds and lacunar infarcts. Growing evidence indicates that SVD might play a significant role in the neurobiology of central nervous system (CNS) neurodegenerative disorders, namely Alzheimer’s disease (AD) and Parkinson’s disease (PD), and neuroinflammatory diseases, such as multiple sclerosis (MS). These disorders share different pathophysiological pathways and molecular mechanisms (i.e., protein misfolding, derangement of cellular clearance systems, mitochondrial impairment and immune system activation) having neurodegeneration as biological outcome. In these diseases, the actual contribution of SVD to the clinical picture, and its impact on response to pharmacological treatments, is not known yet. Due to the high frequency of SVD in adult-aged patients, it is important to address this issue. In this review, we report preclinical and clinical data on the impact of SVD in AD, PD and MS, with the main aim of clarifying the predictability of SVD on clinical manifestations and treatment response.
Low Replicative Stress Triggers Cell-Type Specific Inheritable Advanced Replication Timing
Authors: Courtot Lilas , Bournique Elodie , Maric Chrystelle , Guitton-Sert Laure , Madrid-Mencía Miguel , Pancaldi Vera , Cadoret Jean-Charles , Hoffmann Jean-Sébastien , Bergoglio Valérie
Published: May 2021

DNA replication timing (RT), reflecting the temporal order of origin activation, is known as a robust and conserved cell-type specific process. Upon low replication stress, the slowing of replication forks induces well-documented RT delays associated to genetic instability, but it can also generate RT advances that are still uncharacterized. In order to characterize these advanced initiation events, we monitored the whole genome RT from six independent human cell lines treated with low doses of aphidicolin. We report that RT advances are cell-type-specific and involve large heterochromatin domains. Importantly, we found that some major late to early RT advances can be inherited by the unstressed next-cellular generation, which is a unique process that correlates with enhanced chromatin accessibility, as well as modified replication origin landscape and gene expression in daughter cells. Collectively, this work highlights how low replication stress may impact cellular identity by RT advances events at a subset of chromosomal domains.
Seasonal Regulation of Metabolism: The Effect of Wintertime Fasting and Autumnal Fattening on Key Central Regulators of Metabolism and the Metabolic Profile of the Raccoon Dog (Nyctereutes Procyonoides)
Authors: Niiranen Laura , Mäkelä Kari A. , Dona Anthony , Krumsiek Jan , Karhu Toni , Mäkinen Markus J. , Thalmann Olaf , Saarela Seppo , Herzig Karl-Heinz
Published: May 2021

Investigations into the mechanisms regulating obesity are frantic and novel translational approaches are needed. The raccoon dog (Nyctereutes procyonoides) is a canid species representing a promising model to study metabolic regulation in a species undergoing cycles of seasonal obesity and fasting. To understand the molecular mechanisms of metabolic regulation in seasonal adaptation, we analyzed key central nervous system and peripheral signals regulating food intake and metabolism from raccoon dogs after autumnal fattening and winter fasting. Expressions of neuropeptide Y (NPY), orexin-2 receptor (OX2R), pro-opiomelanocortin (POMC) and leptin receptor (ObRb) were analyzed as examples of orexigenic and anorexigenic signals using qRT-PCR from raccoon dog hypothalamus samples. Plasma metabolic profiles were measured with 1H NMR-spectroscopy and LC-MS. Circulating hormones and cytokines were determined with canine specific antibody assays. Surprisingly, NPY and POMC were not affected by the winter fasting nor autumn fattening and the metabolic profiles showed a remarkable equilibrium, indicating conserved homeostasis. However, OX2R and ObRb expression changes suggested seasonal regulation. Circulating cytokine levels were not increased, demonstrating that the autumn fattening did not induce subacute inflammation. Thus, the raccoon dog developed seasonal regulatory mechanisms to accommodate the autumnal fattening and prolonged fasting making the species unique in coping with the extreme environmental challenges.
Molecular Research on Platelet Activity in Health and Disease 2.0
Authors: Catani Maria Valeria , Savini Isabella , Tullio Valentina , Gasperi Valeria
Published: May 2021

Hsia and collaborators [...]