bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Network Pharmacology and GC-MS–Based Metabolomics to Investigate the Effect of Xiang-Su Volatile Oil Against Menopausal Depression
Authors: Li Yao , Yang Xinyi , Chen Shanshan , Wu Lei , Zhou Jinyong , Jia Keke , Ju Wenzheng
Published: December 2021

Menopausal depression perplexes a great number of women in later life. Xiangfu-Zisu (Xiang-Su), a traditional Chinese herbal pair composed of rhizomes of Cyperus rotundus L. (Xiangfu) and leaves of Perilla frutescens (L.) Britt. (Zisu), is frequently reported with antidepressant-like effects. The volatile oil from Xiangfu and Zisu has shown good antidepressant action, but its mechanism is still unclear. This study aimed to investigate the pharmacological mechanism of Xiang-Su (XS) volatile oil against menopausal depression through gas chromatography–mass spectrometry (GC-MS)-based network pharmacology and metabolomics. First, ADME screening was performed on actual detected components of XS volatile oil to obtain active constituents, and then duplicates of active constituent–related targets and menopausal depression–related targets were collected. These duplicates were considered as targets for XS volatile oil against menopausal depression, followed by GO and KEGG enrichment analyses. It showed that a total of 64 compounds were identified in XS volatile oil, and 38 active compounds were screened out. 42 overlapping genes between 144 compound-related genes and 780 menopausal depression–related genes were obtained. Results showed that targets of SLC6A4 and SLC6A3, regulation of serotonergic and dopaminergic synapses, were involved in the antidepressant mechanism of XS volatile oil. Next, antidepressant-like effect of XS volatile oil was validated in menopausal rats by ovariectomy (OVX) combined with chronic unpredictable mild stress (CUMS). Behavioral tests, biochemical analysis, and GC-MS–based non-targeted plasma metabolomics were employed to validate the antidepressant effect of XS volatile oil. Experimental evidence demonstrated that XS volatile oil reversed behavioral parameters in the sucrose preference test (SPT), open-field test (OFT), forced swim test (FST), and serum estradiol levels in OVX rats. Furthermore, results of metabolomics indicated that XS volatile oil mainly acts on regulating metabolic pathways of phenylalanine, tyrosine and tryptophan biosynthesis, tyrosine metabolism, and tryptophan metabolism, which were corresponding with the above-predicted results. These data suggest that network pharmacology combined with metabolomics provides deep insight into the antidepressant effect of XS volatile oil, which includes regulating key targets like SLC6A4 and SLC6A3, and pathways of serotonergic and dopaminergic synapses.
Multi-Omics Analysis of Microglial Extracellular Vesicles From Human Alzheimer’s Disease Brain Tissue Reveals Disease-Associated Signatures
Authors: Cohn Whitaker , Melnik Mikhail , Huang Calvin , Teter Bruce , Chandra Sujyoti , Zhu Chunni , McIntire Laura Beth , John Varghese , Gylys Karen H. , Bilousova Tina
Published: December 2021

Alzheimer’s disease (AD) is the most common cause of dementia, yet there is no cure or diagnostics available prior to the onset of clinical symptoms. Extracellular vesicles (EVs) are lipid bilayer-delimited particles that are released from almost all types of cell. Genome-wide association studies have linked multiple AD genetic risk factors to microglia-specific pathways. It is plausible that microglia-derived EVs may play a role in the progression of AD by contributing to the dissemination of insoluble pathogenic proteins, such as tau and Aβ. Despite the potential utility of EVs as a diagnostic tool, our knowledge of human brain EV subpopulations is limited. Here we present a method for isolating microglial CD11b-positive small EVs from cryopreserved human brain tissue, as well as an integrated multiomics analysis of microglial EVs enriched from the parietal cortex of four late-stage AD (Braak V-VI) and three age-matched normal/low pathology (NL) cases. This integrated analysis revealed 1,000 proteins, 594 lipids, and 105 miRNAs using shotgun proteomics, targeted lipidomics, and NanoString nCounter technology, respectively. The results showed a significant reduction in the abundance of homeostatic microglia markers P2RY12 and TMEM119, and increased levels of disease-associated microglia markers FTH1 and TREM2, in CD11b-positive EVs from AD brain compared to NL cases. Tau abundance was significantly higher in AD brain-derived microglial EVs. These changes were accompanied by the upregulation of synaptic and neuron-specific proteins in the AD group. Levels of free cholesterol were elevated in microglial EVs from the AD brain. Lipidomic analysis also revealed a proinflammatory lipid profile, endolysosomal dysfunction, and a significant AD-associated decrease in levels of docosahexaenoic acid (DHA)-containing polyunsaturated lipids, suggesting a potential defect in acyl-chain remodeling. Additionally, four miRNAs associated with immune and cellular senescence signaling pathways were significantly upregulated in the AD group. Our data suggest that loss of the homeostatic microglia signature in late AD stages may be accompanied by endolysosomal impairment and the release of undigested neuronal and myelin debris, including tau, through extracellular vesicles. We suggest that the analysis of microglia-derived EVs has merit for identifying novel EV-associated biomarkers and providing a framework for future larger-scale multiomics studies on patient-derived cell-type-specific EVs.
Functional Identification and Characterization of Leucokinin and Its Receptor in the Fall Webworm, Hyphantria cunea
Authors: Sun Lili , Ma Heting , Gao Yuan , Wang Zhiying , Cao Chuanwang
Published: December 2021

Neuropeptides function as central neuromodulators and circulating hormones that modulate insect behavior and physiology. Leucokinin (LK) is an intercellular signaling molecule that mediates many physiological and behavioral processes. However, the functions of LK associated with environmental stress and feeding behavior in the fall webworm, Hyphantria cunea, is little known. Our primary objective is to understand the function of LK and LK receptor (LKR) neuroendocrine system in H. cunea. In the present study, the results showed that LK/LKR are expressed at different developmental stages and in various tissues of H. cunea. A candidate receptor–ligand pairing for LK was identified in the larval transcriptome of H. cunea. In a heterologous expression system, the calcium assay was used to demonstrate that LKR is activated by HcLKs in a dose-dependent manner, with 50% effective concentration (EC50) values of 8.44–90.44nM. Knockdown of HcLK and HcLKR by microinjecting target-specific dsRNA leads to several effects in H. cunea, including feeding promotion, increase in resistance to desiccation and starvation stress, and regulation of water homeostasis. The transcript levels of HILP2 (except in the LK knockdown group), HILP5, and HILP8 increased, whereas those of HILP3, HILP4, and HILP6 decreased; HILP1, HILP2 (in the LK knockdown group), and HILP7 gene expression was not influenced after LK and LKR knockdown. Variations in mRNA expression levels in insulin-like peptide genes in the knockdown larvae suggest an essential role of these genes in survival in H. cunea. To our knowledge, the present study is the first comprehensive study of LK and LKR – from gene to behavior – in H. cunea.
Rhythmic Clock Gene Expression in Atlantic Salmon Parr Brain
Authors: Bolton Charlotte M. , Bekaert Michaël , Eilertsen Mariann , Helvik Jon Vidar , Migaud Herve
Published: December 2021

To better understand the complexity of clock genes in salmonids, a taxon with an additional whole genome duplication, an analysis was performed to identify and classify gene family members (clock, arntl, period, cryptochrome, nr1d, ror, and csnk1). The majority of clock genes, in zebrafish and Northern pike, appeared to be duplicated. In comparison to the 29 clock genes described in zebrafish, 48 clock genes were discovered in salmonid species. There was also evidence of species-specific reciprocal gene losses conserved to the Oncorhynchus sister clade. From the six period genes identified three were highly significantly rhythmic, and circadian in their expression patterns (per1a.1, per1a.2, per1b) and two was significantly rhythmically expressed (per2a, per2b). The transcriptomic study of juvenile Atlantic salmon (parr) brain tissues confirmed gene identification and revealed that there were 2,864 rhythmically expressed genes (p < 0.001), including 1,215 genes with a circadian expression pattern, of which 11 were clock genes. The majority of circadian expressed genes peaked 2 h before and after daylight. These findings provide a foundation for further research into the function of clock genes circadian rhythmicity and the role of an enriched number of clock genes relating to seasonal driven life history in salmonids.
The Insulin-Sensitizer Pioglitazone Remodels Adipose Tissue Phospholipids in Humans
Authors: Palavicini Juan P. , Chavez-Velazquez Alberto , Fourcaudot Marcel , Tripathy Devjit , Pan Meixia , Norton Luke , DeFronzo Ralph A. , Shannon Christopher E.
Published: December 2021

The insulin-sensitizer pioglitazone exerts its cardiometabolic benefits in type 2 diabetes (T2D) through a redistribution of body fat, from ectopic and visceral areas to subcutaneous adipose depots. Whereas excessive weight gain and lipid storage in obesity promotes insulin resistance and chronic inflammation, the expansion of subcutaneous adipose by pioglitazone is associated with a reversal of these immunometabolic deficits. The precise events driving this beneficial remodeling of adipose tissue with pioglitazone remain unclear, and whether insulin-sensitizers alter the lipidomic composition of human adipose has not previously been investigated. Using shotgun lipidomics, we explored the molecular lipid responses in subcutaneous adipose tissue following 6months of pioglitazone treatment (45mg/day) in obese humans with T2D. Despite an expected increase in body weight following pioglitazone treatment, no robust effects were observed on the composition of storage lipids (i.e., triglycerides) or the content of lipotoxic lipid species (e.g., ceramides and diacylglycerides) in adipose tissue. Instead, pioglitazone caused a selective remodeling of the glycerophospholipid pool, characterized by a decrease in lipids enriched for arachidonic acid, such as plasmanylethanolamines and phosphatidylinositols. This contributed to a greater overall saturation and shortened chain length of fatty acyl groups within cell membrane lipids, changes that are consistent with the purported induction of adipogenesis by pioglitazone. The mechanism through which pioglitazone lowered adipose tissue arachidonic acid, a major modulator of inflammatory pathways, did not involve alterations in phospholipase gene expression but was associated with a reduction in its precursor linoleic acid, an effect that was also observed in skeletal muscle samples from the same subjects. These findings offer important insights into the biological mechanisms through which pioglitazone protects the immunometabolic health of adipocytes in the face of increased lipid storage.
Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss
Authors: Wang Chang’an , Su Baohui , Lu Shaoxia , Han Shicheng , Jiang Haibo , Li Zhuang , Liu Yang , Liu Hongbai , Yang Yuhong
Published: December 2021

This study aimed to demonstrate the effects of dietary glutathione (GSH) on growth, intestinal antioxidant capacity, histology, gene expression, and microbiota in juvenile triploid rainbow trout (Oncorhynchus mykiss). Different diets (G0-control, G100, G200, G400, and G800) containing graded levels of GSH (0, 100, 200, 400, and 800mgkg−1) were fed to triplicate groups of 30 fish (initial mean weight 4.12±0.04g) for 56days. G400 had significantly improved weight gain and feed conversion rate. Based on the broken-line regression analysis, the optimum dietary GSH level was 447.06mgkg−1. Catalase and superoxide dismutase activities were significantly higher in G200–G800. G200 had significantly lower malondialdehyde content. The height of the intestinal muscular layer in G400 was significantly higher than that of the control group. Intestinal PepT1 and SLC1A5 gene expression was significantly increased, and the highest was observed in G400. TNF-α, IL-1β, IL-2, and IL-8 expression were significantly decreased than that of G0. Next-generation sequencing of the 16S rDNA showed a significant difference in alpha diversity whereas no differences in beta diversity. On the genus level, LefSe analysis of indicator OTUs showed Ilumatobacter, Peptoniphilus, Limnobacter, Mizugakiibacter, Chelatococcus, Stella, Filimonas, and Streptosporangium were associated with the treatment diet, whereas Arcobacter, Ferrovibrio, Buchnera, Chitinophaga, Stenotrophobacter, Solimonadaceae, Polycyclovorans, Rhodococcus, Ramlibacter, and Azohydromonas were associated with the control diet. In summary, feeding juvenile triploid O. mykiss 200–800mgkg−1 GSH improved growth and intestinal health.
Genome-Wide Analysis of the Cytochrome P450 Gene Family Involved in Salt Tolerance in Gossypium hirsutum
Authors: Sun Kangtai , Fang Hui , Chen Yu , Zhuang Zhimin , Chen Qi , Shan Tingyu , Khan Muhammad Kashif Riaz , Zhang Jun , Wang Baohua
Published: December 2021

Plant cytochrome P450 (P450) participates in a wide range of biosynthetic reactions and targets a variety of biological molecules. These reactions lead to various fatty acid conjugates, plant hormones, secondary metabolites, lignin, and various defensive compounds. In our previous research, transcriptome analysis was performed on the salt-tolerant upland cotton “Tongyan No. 1.” Many differentially expressed genes (DEGs) belong to the P450 family, and their domains occur widely in plants. In this current research, P450 genes were identified in Gossypium hirsutum with the aid of bioinformatics methods for investigating phylogenetic relations, gene structure, cis-elements, chromosomal localization, and collinearity within a genome. qRT-PCR was conducted to analyze P450 gene expression patterns under salt stress. The molecular weights of the 156 P450 genes were in the range of 5,949.6–245,576.3 Da, and the length of the encoded amino acids for all the identified P450 genes ranged from 51 to 2,144. P450 proteins are divided into four different subfamilies based on phylogenetic relationship, gene structure, and chromosomal localization of gene replication. The length of P450 genes in upland cotton differs greatly, ranging from 1,500 to 13,000 bp. The number of exons in the P450 family genes ranged from 1 to 9, while the number of introns ranged from 0 to 8, and there were similar trends within clusters. A total of 31 cis-acting elements were identified by analyzing 1,500 bp promoter sequences. Differences were found in cis-acting elements among genes. The consistency between qRT-PCR and previous transcriptome analysis of salt tolerance DEGs indicated that they were likely to be involved in the salt tolerance of cotton seedlings. Our results provide valuable information on the evolutionary relationships of genes and functional characteristics of the gene family, which is beneficial for further study of the cotton P450 gene family.
RETRACTED: Use of Ore-Derived Humic Acids With Diverse Chemistries to Elucidate Structure-Activity Relationships (SAR) of Humic Acids in Plant Phenotypic Expression
Authors: Lamar Richard T. , Monda Hiarhi , Sleighter Rachel
Published: December 2021

We report the results of a structure-activity relationship study that was undertaken to identify humic substance chemistries that drive the plant biostimulant response. The effects of seven extensively chemically characterized, ore-derived humic acids (HA) on corn seedling biomass and root and shoot morphological parameters were investigated. Chemometric analyses were then conducted to identify correlations between HA chemical features and plant biomass and morphological characteristics. The primary chemical driver of plant biomass and morphology was the ratio between HA electron accepting capacity (EAC) and electron donating capacity (EDC). The HA electron accepting capacity is found in quinones and semiquinone free radicals, while the HA electron donating capacity is found in polyphenolics and glycosylated polyphenolics. Based on our results, we propose a mechanism of action for ore-derived HA plant biostimulation that involves the interplay of pro-oxidants, in the form of quinones and semiquinone radicals, and antioxidants, in the form of polyphenols and possibly glycones and carbohydrates. The quinones/semiquinones initiate an oxidative stress response via the stimulation of transmembrane electron flow that results in both reactive oxygen species (ROS) production (i.e., an oxidative burst) and membrane depolarization, the latter of which allows Ca2+ flux from the apoplast into the cytoplasm. Based on the magnitude of depolarization, a specific cytoplasmic Ca2+ signature is produced. As a secondary messenger Ca2+, via binding to Ca2+− sensor proteins, transmits the signature signal, resulting in specific intracellular responses that include changes to plant morphology. The greater the EAC, the greater the ROS production and magnitude of plasma membrane depolarization and resulting stress response. The HA antioxidants are able to scavenge and quench the ROS and thus modulate the intensity and extent of the stress response to greater or lesser degrees, based on their concentrations and radical scavenging efficiencies, and thus modify the Ca2+ signature and ultimately the intracellular molecular responses.
Non-parental Adults and Sexual Health Behaviors Among Young Minority Men: A Qualitative Examination
Authors: Sterrett-Hong Emma M. , DeBow Joseph , Caton Erica , Harris Matthew , Brewer Russell , Roberts Erin , Marchal Madeline , Tauzer Marjorie , Arnold Emily A.
Published: December 2021

Young Black and Latino sexual minority men (YBLSM) exhibit disproportionately high rates of negative sexual health outcomes, including HIV and other sexually transmitted infections, compared to other groups, partly due to relatively higher rates of exposure to a host of socio-structural risk factors (e.g., unstable housing and under-employment). However, an under-studied interpersonal resource exists for many YBLSM, non-parental adults (NPAs, i.e., adults who act as role models and provide social support), who may be able to influence contextual (e.g., unemployment) and individual (e.g., reduced health expectations) factors underlying sexual health disparities.

Aims: This study sought to examine the role of NPAs in factors that affect sexual health behaviors and in supporting those health behaviors directly, among YBLSM living in a mid-sized city in the southern United States. A total of n=20 participants, n=10 YBLSM (ages 16 to 22), and n=10 NPAs (ages 26 to 52) were interviewed using semi-structured guides to examine NPA involvement in the lives of YBLSM from both sides of the relationship. The research team used a framework analysis approach to iteratively identify and define meaningful codes and sub-codes. Both YBLSM and NPAs described NPAs helping YBLSM through role modeling and social support in a variety of areas found to affect sexual health behaviors, such as housing instability and psychological distress, as well as in specific behaviors, such as condom use and HIV medication adherence. Given the multiple socio-structural obstacles facing YBLSM and their multifaceted relationships with NPAs, NPAs may be a promising resource to help address these impediments to health. Partnering more intentionally with NPAs is a potentially promising strategy to help reduce HIV-related disparities affecting YBLSM that is worthy of additional empirical attention.
Populated Places and Conspicuous Consumption: High Population Density Cues Predict Consumers’ Luxury-Linked Brand Attitudes
Authors: Otterbring Tobias , Folwarczny Michał , Tan Lynn K. L.
Published: December 2021

Population density has been identified as an ecological factor with considerable behavioral implications. The present research aimed to examine whether the mere perception of more (vs. less) populated places can change consumers’ luxury-linked brand attitudes. To this end, we experimentally manipulated consumers’ perceptions of population density using pictorial exposure to high (vs. low) population density cues. The results revealed a significant interaction between manipulated population density and perceived brand luxury on brand attitudes. Specifically, exposure to high rather than low population density cues resulted in more positive (negative) attitudes toward brands deemed to be more (less) luxurious. These findings support our prediction that high population density cues can shift people’s perceptions in consumption contexts linked to luxury. Our work contributes to the growing stream of literature on population density and suggests that this (geo-) demographic factor can exert important downstream effects on consumer behavior.
Approaching Collectivity Collectively: A Multi-Disciplinary Account of Collective Action
Authors: Thonhauser Gerhard , Weichold Martin
Published: December 2021

There has been considerable progress in investigating collective actions in the last decades. However, the real progress is different from what many scholars take it to be. It lies in the fact that there is by now a wealth of different approaches from a variety of fields. Each approach has carved out fruitful mechanisms for explaining collective action, but is also faced with limitations. Given that situation, we submit that the next step in investigating collective action is to acknowledge the plurality of approaches and bring them into dialogue. With this aim in mind, the present article discusses the strengths and weaknesses of some of the to our mind most relevant approaches to collective action in current debates. We begin with the collective intentionality framework, the team reasoning approach, and social identity theory. Then, we move to ecological social psychology, participatory sense-making, and, through the lenses of those frameworks, dynamical systems theory. Finally, we discuss practice theory. Against this background, we provide a proposal for a synthesis of the successful explanatory mechanisms as they have been carved out by the different research programs. The suggestion is, roughly, to understand collective action as dynamical interaction of a self-organizing system with its environment, shaped by a process of collective sense-making.
Does Sound Influence Perceived Duration of Visual Motion?
Authors: Carlini Alessandro , Bigand Emmanuel
Published: December 2021

Multimodal perception is a key factor in obtaining a rich and meaningful representation of the world. However, how each stimulus combines to determine the overall percept remains a matter of research. The present work investigates the effect of sound on the bimodal perception of motion. A visual moving target was presented to the participants, associated with a concurrent sound, in a time reproduction task. Particular attention was paid to the structure of both the auditory and the visual stimuli. Four different laws of motion were tested for the visual motion, one of which is biological. Nine different sound profiles were tested, from an easier constant sound to more variable and complex pitch profiles, always presented synchronously with motion. Participants’ responses show that constant sounds produce the worst duration estimation performance, even worse than the silent condition; more complex sounds, instead, guarantee significantly better performance. The structure of the visual stimulus and that of the auditory stimulus appear to condition the performance independently. Biological motion provides the best performance, while the motion featured by a constant-velocity profile provides the worst performance. Results clearly show that a concurrent sound influences the unified perception of motion; the type and magnitude of the bias depends on the structure of the sound stimulus. Contrary to expectations, the best performance is not generated by the simplest stimuli, but rather by more complex stimuli that are richer in information.
Social Virtual Reality (VR) Involvement Affects Depression When Social Connectedness and Self-Esteem Are Low: A Moderated Mediation on Well-Being
Authors: Lee Hyun-Woo , Kim Sanghoon , Uhm Jun-Phil
Published: December 2021

While social interaction and play in a VR environment are becoming ever more popular, little is known about how social VR games affect users. The purpose of this study was to elucidate the role of several contingent factors in social VR games by modeling the relationships between involvement, well-being, depression, self-esteem, and social connectedness. A conditional process-moderated mediation model of the measured variables was analyzed with 220 pieces of collected data. The result showed that: (1) the direct effect of involvement on well-being was significant, and (2) the index of moderated mediation involving depression, self-esteem, and social connectedness was significant. We conclude that high levels of involvement in social VR games by socially isolated users with low self-esteem can negatively affect their well-being. The findings of this study contribute in several ways to our understanding of the effect of social VR games upon users and provide important practical implications.
Screening Social Anxiety in Adolescents Through the Eyes of Their Carers
Authors: Garcia-Lopez Luis-Joaquin , Espinosa-Fernandez Lourdes , Muela-Martinez Jose-Antonio , Piqueras Jose Antonio
Published: December 2021

Despite the availability of efficacious treatment and screening protocols, social anxiety disorder (SAD) in adolescents is considerably under-detected and undertreated. Our main study objective was to examine a brief, valid, and reliable social anxiety measure already tested to serve as self-report child measure but administered via Internet aimed at listening to the ability of his or her parent to identify social anxiety symptomatology in his or her child. This parent version could be used as a complementary measure to avoid his or her overestimation of children of social anxiety symptomatology using traditional self-reported measures. We examined the psychometric properties of brief and valid social anxiety measure in their parent format and administered via the Internet. The sample included 179 parents/legal guardians of adolescents (67% girls) with a clinical diagnosis of SAD (mean age: 14.27; SD = 1.33). Findings revealed good factor structure, internal consistency, and construct validity. Data support a single, strength-based factor on the SPAIB-P, being structure largely invariant across age and gender. The limited number of adolescents with a performance-only specifier prevented examining the utility of scale to screen for this recently established specifier. It is crucial to evaluate if these results generalize to different cultures and community samples. The findings suggest that the SPAIB-P evidences performance comparable with child-reported measure. Parents can be reliable reports of the social anxiety symptomatology of the adolescent. The SPAIB-P may be useful for identifying clinically disturbed socially anxious adolescents.
Development and Validation of an Unethical Professional Behavior Tendencies Scale for Student Teachers
Authors: Wang Jing , Wang Xin-qiang , Li Jia-yuan , Zhao Cui-rong , Liu Ming-fan , Ye Bao-juan
Published: December 2021

Teacher’s unethical professional behaviors affect students’ physical and mental health. Prevention should start with student teachers, but empirical research is lacking in China. This study surveyed over 2,000 student teachers from China to examine the psychometric properties of a student teachers’ unethical professional behavior tendencies scale which revised by primary and secondary school teachers’ unethical professional behavior tendencies scale. Exploratory and confirmatory factor analysis confirmed that a bi-factor model fit the data best. The final student teachers’ unethical professional behavior tendencies scale comprised four subscales, including a general factor (unethical professional behaviors) and four special factors (perfunctory attitude and carelessness, insults and discrimination, unfairness, and using power for personal gain). The student teachers’ unethical professional behavior tendencies scale correlated negatively with their professional ethical values and positively with perceived frequency of unethical professional behaviors of college teachers around them. The data supported the scale’s measurement invariance across gender, and male student teachers scored significantly higher on unethical professional behavior tendencies than female student teachers. The findings suggest that the student teachers’ unethical professional behavior tendencies scale is a useful instrument for assessing student teachers’ unethical professional behaviors in China.
Positive Psychology 2.0 in a Foreign Language Classroom: Students’ Emotional Experience in English Classroom Interaction in China
Authors: Wang Yingna , Marecki Mateusz
Published: December 2021

The second wave of positive psychology (PP 2.0) focuses on the way positive and negative psychology complement each other in social contexts. It offers a balanced interactive model that aims at enhancing the optimal learning outcome through the interplay of positive and negative emotions. Building on a large qualitative study of students’ and teachers’ experiences in EFL classrooms in China, this paper argues that adopting the principles of PP 2.0 could deepen our understanding of learners’ emotional experience in SLA. Using one illustrative case, it shows the dynamic and complexity of students’ shifting emotions as they interact in the classroom over a span of 2 months. One major finding is that the students’ positive emotions could transcend negative emotions and influence their engagement in classroom interaction. This study contributes to the existing research into emotional experiences of classroom interaction that integrates the observable, reflective, and participatory. It draws on interrelated sets of data, including a student and teacher profile questionnaire, classroom observation and recording, student and teacher reflective journals documenting their classroom interaction experiences, and stimulated recall interviews based on recordings and reflective journals. The study in the first place has implications for English teachers and teacher trainers in China and abroad as well as researchers interested in the role of emotional experience in English language learning and teaching.
Case Report: Repeated Series of Ketamine Infusions in Patients With Treatment-Resistant Depression: Presentation of Five Cases
Authors: Gałuszko-Wȩgielnik Maria , Włodarczyk Adam , Cubała Wiesław Jerzy , Wilkowska Alina , Górska Natalia , Słupski Jakub
Published: December 2021

Purpose: Approximately 30% of patients with major depressive disorder (MDD) are treatment resistant. There is an unquestionable need for new treatment strategies. Subanesthetic doses of intravenous (IV) ketamine have a rapid antidepressant effect in treatment-resistant depression (TRD). This paper describes the efficacy of repeated series of intravenous ketamine infusions as an add-on treatment in five TRD inpatients.

Methods: Eligible patients aged 43–63 were given eight ketamine infusions as an add-on treatment for patients with MDD. The subjects have readministered the intervention due to worsening depressive symptoms.

Results: Of the five inpatients given ketamine as a series of eight infusions, one underwent three, and four had two treatment series. Four patients achieved remission after first series and three after the second series of ketamine infusions. The adverse reactions were mild and transient with no sequelae.

Limitations: Presented case series applies to short-term intervention with IV ketamine as an add-on therapy. The results cannot be generalized to the long-term maintenance treatment nor other ketamine formulations as well as different administration schedules and dosing.

Conclusions: This case series showed efficacy and safety of the repeated series of IV ketamine treatment in TRD in MDD and bipolar disorder type I. The subsequent interventions were safe and observed adverse events were mild and transient. Interestingly, the IV ketamine treatment at successive administrations seems to alter the major depression severity of the next affective episode. There is a critical need for further research regarding IV ketamine treatment effectiveness and long-term safety in future studies.
Risk and Protective Factors Associated With Health-Related Quality of Life of Parents With Mental Illness
Authors: Radicke Alina , Sell Marlit , Adema Bonnie , Daubmann Anne , Kilian Reinhold , Busmann Mareike , Winter Sibylle M. , Lambert Martin , Wegscheider Karl , Wiegand-Grefe Silke
Published: December 2021

Purpose: Health-related quality of life (HRQoL) can be reduced in parents with mental illness (mental illness) who face the dual demands of disabling symptoms and their impact on family, social, and occupational life. This study aimed at analyzing the influence of various factors on HRQoL in parents with mental illness.

Method: Baseline data of the German randomized controlled multicenter project CHIMPS (children of parents with mental illness) was used for analyses. The final sample consisted of n = 208 parents with mental illness and n = 197 children and adolescents aged 8–18 years. HRQoL was assessed with the EQ-5D.

Results: Parents with mental illness reported significantly lower global and specific HRQoL than the German reference population. They were least satisfied with aspects that relate to anxiety and depression followed by usual activities, pain and discomfort. Better global HRQoL was primarily associated with self-reported physical and mental health, as well as adaptive coping behavior. Associations with mobility, self-care, usual activity, pain and discomfort, anxiety and depression were analyzed and discussed.

Conclusions: HRQoL in parents with mental illness is reduced. Clinical interventions should focus on the alleviation of mental health symptoms and probably somatic symptoms and promote adaptive coping skills.
DXA-Derived Adiposity and Lean Indices for Management of Cardiometabolic and Musculoskeletal Frailty: Data Interpretation Tricks and Reporting Tips
Authors: Minetto Marco A. , Busso Chiara , Lalli Piera , Gamerro Giulia , Massazza Giuseppe
Published: December 2021

The proper assessment and follow-up of obesity and sarcopenia are relevant for the proper management of the complications of cardiometabolic and musculoskeletal frailty. A total body dual-energy X-ray absorptiometry (DXA) scan should be systematically incorporated in the rehabilitative routine management of patients with obesity and sarcopenia. In the former patients, the total body DXA can be used to assess the fat tissue amount and distribution, while in the latter patients, it can be used to quantify the reduction of appendicular lean mass and to investigate the inter-limb lean mass asymmetry. This tutorial article provides an overview of different DXA-derived fat and lean indices and describes a step-by-step procedure on how to produce a complete DXA report. We suggest that the systematic incorporation of these indices into routine examinations of the patients with obesity and sarcopenia can be useful for identifying the patients at risk for cardiometabolic and neuromuscular impairment-related comorbidities and for evaluating the effectiveness of pharmacological and rehabilitative interventions.
The Experience of Locomotor Training From the Perspectives of Therapists and Parents of Children With Cerebral Palsy
Authors: Pool Dayna , Elliott Catherine , Willis Claire , Thornton Ashleigh
Published: December 2021

Objective: The objective of this study was to explore the experiences of intensive locomotor training from the perspective of therapists and parents of children with cerebral palsy.

Design: A qualitative study using semi-structured interviews was employed to capture perspectives following an intensive locomotor training intervention. Data were analyzed thematically, systematically coding and interpreted by grouping information into themes and sub-theme categories.

Participants: Five therapists and seven parents of children with high daily physical assistance and equipment needs participated in the study.

Setting: A pediatric tertiary hospital.

Results: Experiences of locomotor training were described with relation to the suitability of locomotor training with sub-themes of intervention length and time, engagement within sessions, the importance of support, and the utility of locomotor training beyond a research context. Motivation for participating in locomotor training was described in relation to the enjoyment of movement and for increasing activity level. The barriers and facilitators who participated in locomotor training provided environmental and personal factor subthemes. Finally, the outcomes from the intervention were related to improvements in physical health, sleep, affect and emotion, and ambulation in daily activities.

Conclusion: The experience of intensive locomotor training from the perspectives of parents of children who have high physical assistance and equipment needs and the therapists providing the intervention was described. Future studies should consider outcome measures beyond motor capacity to quantify the perceived outcomes of interventions that are meaningful to families.
Investigating the Associations Between Child Autistic Symptoms, Socioeconomic Context, and Family Life: A Pilot Study
Authors: Koziarz Frank , Roncadin Caroline , Kata Anna , Duku Eric , Cauwenbergs Amber , Mahoney William , Di Rezze Briano , Anderson Colleen , Drmic Irene , Eerkes Judy , Dekker Kathleen , Georgiades Katholiki , Hoult Lorraine , Kraus de Camargo Olaf , Ng Olivia , Rosenbaum Peter , Mesterman Ronit , Gentles Stephen J. , Robertson Sue , Bennett Teresa , Georgiades Stelios
Published: December 2021

Objective: The day-to-day experience of families with an Autistic child may be shaped by both, child characteristics and available resources, which often are influenced by the socioeconomic context of the family. Using a socioecological approach, this study explored the quantitative associations between child autistic symptoms, family socioeconomic status, and family life.

Methods: Data came from the Pediatric Autism Research Cohort—PARC Study (pilot). Parents of children with a recent diagnosis of autism completed a set of assessments, including the Autism Family Experience Questionnaire, Autism Impact Measure, and a Sociodemographic Questionnaire. A series of multiple, iterative linear regression models were constructed to ascertain quantitative associations between child autistic symptoms, socioeconomic context, and family life.

Results: A total of 50 children (mean age: 76 months; SD: 9.5 months; and 84% male) with data on the variables of interest were included in the analysis. The frequency of child autistic symptoms was associated with family life outcomes (p = 0.02 and R2 = 24%). Once autistic symptom frequency, symptom impact, and sociodemographic variables were considered, parents of higher educational attainment reported worse family life outcomes compared to their lesser-educated counterparts. This cumulative regression model had considerable explanatory capability (p = 0.01, R2 = 40%).

Conclusion: This study demonstrates the utility of using a socioecological approach to examine the dynamic interplay between child characteristics and family circumstances. Our findings suggest that family life for parents (of an autistic child) who have obtained higher education is reported (by the parents themselves) as less satisfactory compared to that of parents without higher education, once adjusted for the autistic symptom frequency of child, symptom impact, and income. These findings can inform the design and delivery of more family-centered care pathways during the years following a diagnosis of autism.
Effectiveness of Self-Monitoring Approach Using Fitness Trackers to Improve Walking Ability in Rehabilitation Settings: A Systematic Review
Authors: Otaka Eri , Oguchi Kazuyo , Kondo Izumi , Otaka Yohei
Published: December 2021

Background: A self-monitoring approach utilizing fitness trackers that provide feedback regarding physical activities has been recently applied to rehabilitation patients to promote voluntary walking activities. Although this approach has been proven to increase physical activity, it is uncertain whether the intervention improves walking ability.

Aim: This review investigated whether the additional self-monitoring approach using activity trackers would improve walking ability in any type of rehabilitation setting.

Methods: A systematic search was performed in four databases [PubMed (MEDLINE), The Cochrane Library, SPORTDiscus, and Cumulative Index to Nursing and Allied Health Literature] to identify studies that examined the self-monitoring approach combined with rehabilitative intervention vs. the same rehabilitative intervention only in participants with any unhealthy conditions. Two review authors independently assessed the eligibility of all the retrieved English literature published from 2009 to 2019, then discussed the final inclusion. The risk of bias was assessed referring to the criteria of the Cochrane Risk of Bias tool. The key findings were synthesized using narrative synthesis. In addition, a quantitative synthesis was conducted when more than two studies investigating the same disease were identified.

Results: Eleven randomized controlled trials satisfied the eligibility criteria, nine of which had a lower risk of bias. The types of diseases included stroke, chronic obstructive pulmonary disease (COPD), cancer, Parkinson's disease, hemophilia, peripheral artery disease, post-total knee arthroplasty, and geriatric rehabilitation. Eight studies reported measures of walking endurance and four reported measures of gait speed. In the quantitative synthesis of two studies investigating COPD, there was a significant between-group difference in terms of changes in the 6-min walking distance from the baseline, which was favorable to the additional self-monitoring intervention group (mean difference: 13.1 m; 95% confidence interval, 1.8–24.5; 2 studies, 124 participants; p = 0.02; I2 = 0%). Other available data revealed no consistent evidence regarding effectiveness of the intervention.

Conclusions: The findings indicate that there is little evidence suggesting the effectiveness of the self-monitoring approach in improving walking ability in rehabilitation settings. However, a weak recommendation for patients with stable COPD was implicated in the quantitative synthesis. Further research would be required to explore the best indications for this self-monitoring approach.

Systematic Review Registration: CRD 42020157695.
Considerations When Applying Large-Scale PIV and PTV for Determining River Flow Velocity
Authors: Jolley Martin J. , Russell Andrew J. , Quinn Paul F. , Perks Matthew T.
Published: December 2021

Large-scale image velocimetry is a novel approach for non-contact remote sensing of flow in rivers. Research within this topic has largely focussed on developing specific aspects of the image velocimetry work-flow, or alternatively, testing specific tools or software using case studies. This has resulted in the development of a multitude of techniques, with varying practice being employed between groups, and authorities. As such, for those new to image velocimetry, it may be hard to decipher which methods are suited for particular challenges. This research collates, synthesises, and presents current understanding related to the application of particle image velocimetry (PIV) and particle tracking velocimetry (PTV) approaches in a fluvial setting. The image velocimetry work-flow is compartmentalised into sub-systems of: capture optimisation, pre-processing, processing, and post-processing. The focus of each section is to provide examples from the wider literature for best practice, or where this is not possible, to provide an overview of the theoretical basis and provide examples to use as precedence and inform decision making. We present literature from a range of sources from across the hydrology and remote sensing literature to suggest circumstances in which specific approaches are best applied. For most sub-systems, there is clear research or precedence indicating how to best perform analysis. However, there are some stages in the process that are not conclusive with one set method and require user intuition or further research. For example, the role of external environmental conditions on the performance of image velocimetry being a key aspect that is currently lacking research. Further understanding in areas that are lacking, such as environmental challenges, is vital if image velocimetry is to be used as a method for the extraction of river flow information across the range of hydro-geomorphic conditions.
Eating While the Eating’s Good: How Fire Creates a Magnet for Grazing Animals
Authors: Leis Sherry A. , Baldwin Carol E.
Published: December 2021

Tallgrass prairie is disappearing because farming and development have replaced it. This ecosystem is home to a unique group of plants, animals, and microbial life. The processes of fire, grazing by animals, and drought are important to the tallgrass prairie. They can influence each other and prairie life. For example, pyric-herbivory is the interaction of fire and grazing on the landscape. Burned areas attract herbivores (plant eaters) like a magnet. After fires, plant growth is nutritious and easy to find. Herbivores prefer grazing recently burned areas, creating patches of different habitats that support many other wildlife species, too. You can see pyric-herbivory in action at the Tallgrass Prairie National Preserve, where fire, cattle, and bison are a part of the preserve’s management team! Healthy tallgrass prairie needs both fire and grazing.
Perspectives on Reducing the National Milk Deficit and Accelerating the Transition to a Sustainable Dairy Value Chain in Zimbabwe
Authors: Chirinda Ngonidzashe , Murungweni Chrispen , Waniwa Addmore , Nyamangara Justice , Tangi Aziza , Peters Michael , Notenbaert An , Burkart Stefan
Published: December 2021

The Zimbabwean dairy industry is massively underperforming, as evidenced by a reduction in milk yield from 262 million liters in 1990 to <37 million liters in 2009 and a steady but slow increase to 82 million liters in 2021. The current demand for milk in Zimbabwe stands at 130 million liters, and there is a national capacity for processing 400 million liters per annum. This study used literature, stakeholder inputs and expert knowledge to provide a perspective on practical options to reduce the national milk deficit and, simultaneously, accelerate the transition to a sustainable dairy value chain in Zimbabwe. Following a discussion on the key barriers and constraints to developing the milk value chain, we explored opportunities to improve the performance of the underperforming smallholder and medium-scale dairy farmers. Specifically, we discussed innovative management, creative policy instruments and alternative technological options to maximize milk production in Zimbabwe. We also highlight the need for an inclusive and creatively organized dairy value chain to optimize stakeholder linkages and improve information flow and equity. Examples of crucial investments and incentive structures for upgrading the existing value chain and monitoring greenhouse gas emissions and carbon uptake are discussed. Furthermore, the socio-economic effects (i.e., profitability, women empowerment and employment creation), milk quality, safety and traceability issues linked to a better organized and performing dairy value chain are highlighted.