bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting new ways for large-scale, high-speed surface functionalization using direct laser interference patterning in a roll-to-roll process
Authors: Zwahr Christoph , Serey Nicolas , Nitschke Lukas , Bischoff Christian , Rädel Ulrich , Meyer Alexandra , Zhu Penghui , Pfleging Wilhelm
Published: May 2023

Direct Laser Interference Patterning (DLIP) is used to texture current collector foils in a roll-to-roll process using a high-power picosecond pulsed laser system operating at either fundamental wavelength of 1064 nm or 2nd harmonic of 532 nm. The raw beam having a diameter of 3 mm @ 1/e
2 is shaped into an elongated top-hat intensity profile using a diffractive so-called FBS®-L element and cylindrical telescopes. The shaped beam is split into its diffraction orders, where the two first orders are parallelized and guided into a galvanometer scanner. The deflected beams inside the scan head are recombined with an F-theta objective on the working position generating the interference pattern. The DLIP spot has a line-like interference pattern with about 15 μm spatial period. Laser fluences of up to 8 J cm−2 were achieved using a maximum pulse energy of 0.6 mJ. Furthermore, an in-house built roll-to-roll machine was developed. Using this setup, aluminum and copper foil of 20 μm and 9 μm thickness, respectively, could be processed. Subsequently to current collector structuring coating of composite electrode material took place. In case of lithium nickel manganese cobalt oxide (NMC 622) cathode deposited onto textured aluminum current collector, an increased specific discharge capacity could be achieved at a C-rate of 1 °C. For the silicon/graphite anode material deposited onto textured copper current collector, an improved rate capability at all C-rates between C/10 and 5 °C was achieved. The rate capability was increased up to 100% compared to reference material. At C-rates between C/2 and 2 °C, the specific discharge capacity was increased to 200 mAh g−1, while the reference electrodes with untextured current collector foils provided a specific discharge capacity of 100 mAh g−1, showing the potential of the DLIP technology for cost-effective production of battery cells with increased cycle lifetime.
Development of a roll-to-roll high-speed laser micro processing machine for preparing through-holed anodes and cathodes of lithium-ion batteries
Authors: Yamada Mitsuru , Soma Naohiko , Tsuta Masaya , Nakamura Susumu , Ando Nobuo , Matsumoto Futoshi
Published: May 2023

Aiming to improve the battery performance of lithium-ion batteries (LIBs), modification of the cathodes and anodes of LIBs using laser beams to prepare through-holes, non-through-holes or ditches arranged in grid and line patterns has been proposed by many researchers and engineers. In this study, a laser processing system attached to rollers, which realizes this modification without large changes in the present mass-production system, was developed. The laser system apparatus comprises roll-to-roll equipment and laser equipment. The roll-to-roll equipment mainly consists of a hollow cylinder with openings on its circumferential surface. Cathode and anode electrodes for LIBs are wound around the cylinder in the longitudinal direction of the electrodes. A pulsed beam reflected from the central axis of the cylinder can continuously open a large number of through-holes in the thin electrodes. Through-holes were formed at a rate of 100 000 holes per second on lithium iron phosphate cathodes and graphite anodes with this system. The through-holed cathodes and anodes prepared with this system exhibited higher C-rate performance than nontreated cathodes and anodes.
Bolzano on aesthetic normativity
Authors: Lopes Dominic McIver
Published: May 2023

A theory of aesthetic normativity states what makes it the case that the fact that an item is beautiful is reason to appreciate it. Aesthetic hedonists characteristically hold that the fact that an item is beautiful is reason to appreciate it because anyone always has reason to do what yields pleasure. Bernard Bolzano was an aesthetic hedonist who is best interpreted as offering a mixed theory of aesthetic normativity.The fact that an item is beautiful is reason to appreciate it because anyone always has reason to do what yields pleasure and because anyone also has reason to do what fosters virtue, the capacity to act well. The argument for this interpretation knits together Bolzano’s aesthetics, his ethics, and his philosophy of science. In doing so, this argument opens up a new perspective on aesthetic normativity.
Lessons Learned From Integrating Anti-Oppression Pedagogy in a Graduate-Level Course in Epidemiology
Authors: Lyons Vivian H , Seiler Jessie , Rowhani-Rahbar Ali , Adhia Avanti
Published: May 2023

Despite the high burden of injury and violence globally and disproportionate burden on marginalized communities, few US schools of public health and departments of epidemiology offer classes focused on injury and violence, and even fewer are taught with an antiracist or anti-oppression framework. Recent years have brought renewed focus to incorporating antiracist and anti-oppression principles to pedagogy. Public health professionals have increasingly grappled with how we teach, conduct research, and advocate for just policies, which are shaped by interlocking systems of oppression. Although all areas of epidemiology are shaped by these structures, motivations for those who study injury and violence ought to be especially keen. In this commentary, we illustrate how anti-oppression can be integrated into course development and delivery with a case study of a graduate-level course at the University of Washington School of Public Health on injury and violence epidemiology. We include feedback from an epidemiology faculty reviewer, as well as narratives from students describing what worked and what did not. We offer our reflections and lessons learned, hoping to encourage others within public health and epidemiology to adopt an anti-oppression framework in developing classes and programs, particularly those related to injury and violence.
SNAP Participation and Medication Adherence Among Older Black Medicaid-Insured Individuals Living With Hypertension
Authors: Ojinnaka Chinedum O , Arteaga Irma , Hodges Leslie , Heflin Colleen
Published: May 2023

BACKGROUND

Black people are more likely to be diagnosed with hypertension and to experience food insecurity and antihypertensive medication non-adherence compared to White people in the U.S. The Supplemental Nutrition Assistance Program (SNAP)—a means-tested program that targets food insecurity has been shown to affect health outcomes. This study analyzed the relationship between SNAP participation and antihypertensive medication adherence among older Black Medicaid-insured individuals.

METHODS

This is a retrospective cohort study using linked 2006–2014 state of Missouri Medicaid and SNAP administrative claims data. Analyses were restricted to older (≥60 years) Black individuals who were continuously enrolled in Medicaid for 12 months following their first observed claim for hypertension at or after age 60 years with at least one pharmacy claim (n = 10,693). Our outcome measure is a dichotomous measure of antihypertensive medication adherence defined using the Proportion of Days Covered (≥80% PDC = 1). The exposure variables are four measures of SNAP participation.

RESULTS

A higher proportion of SNAP participants were adherent to their antihypertensive medications compared to non-SNAP participants (43.5% vs. 32.0%). On multivariable analyses, compared to non-SNAP participants there was an increased likelihood of antihypertensive medication adherence among SNAP participants (prevalence ratio [PR] = 1.25; 95% confidence interval [CI] = 1.16–1.35). Compared to those who participated in SNAP for 1–3 months during the 12-month continuous enrollment, there was an increased likelihood of antihypertensive medication adherence among those who were enrolled for 10–12 months (PR = 1.41; 95% CI = 1.08–1.85).

CONCLUSIONS

Medicaid-insured older Black adults who were SNAP participants had a higher likelihood of antihypertensive medication adherence compared to non-SNAP participants.
Outpatient-Based High-Volume Liposuction: A Retrospective Review of 310 Consecutive Patients
Authors: Golpanian Samuel , Rahal George A , Rahal William J
Published: May 2023

Background

Currently, the definition of large-volume liposuction is the removal of 5 L or more of total aspirate. Higher volumes of lipoaspirate come into consideration with higher BMIs, because more than 5 L is often required to achieve a satisfactory aesthetic result. The boundaries of what lipoaspirate volume is considered safe are based on historical opinion and are constantly in question.

Objectives

Because to date there have been no scientific data available to support a specific safe maximum volume of lipoaspirate, the authors discuss necessary conditions for safe high-volume lipoaspirate extraction.

Methods

This retrospective study included 310 patients who had liposuction of ≥5 L over a 30-month period. All patients had 360° liposuction alone or in combination with other procedures.

Results

Patient ages ranged from 20 to 66 with a mean age of 38.5 (SD = 9.3). Average operative time was 202 minutes (SD = 83.1). Mean total aspirate was 7.5 L (SD = 1.9). An average of 1.84 L (SD = 0.69) of intravenous fluids and 8.99 L (SD = 1.47) of tumescent fluid were administered. Urine output was maintained above 0.5 mL/kg/hr. There were no major cardiopulmonary complications or cases requiring blood transfusion.

Conclusions

High-volume liposuction is safe if proper preoperative, intraoperative, and postoperative protocols and techniques are employed. The authors believe that this bias should be modified and that sharing their experience with high-volume liposuction may help guide other surgeons to incorporate this practice with confidence and safety for better patient outcomes.

Level of Evidence: 3
Studies on biosynthetic enzymes leading to structural and functional diversity of microbial natural products
Authors: Dairi Tohru
Published: May 2023

The primary metabolic pathways, for the most part, have been disclosed in Escherichia coli and Saccharomyces cerevisiae. These pathways were believed to be common among all microorganisms. However, after discovery of an alternative pathway for biosynthesis of isopentenyl diphosphate, the methylerythritol phosphate pathway, genome mining of alternative biosynthetic pathways for primary metabolites has been performed. My collaborators and I focused on the biosynthetic pathways of menaquinone and peptidoglycan because some microorganisms lack ortholog genes of the known biosynthetic pathways for these compounds. I also studied biosynthetic enzymes for secondary metabolites produced by actinomycetes and fungi because they include many unique enzymes. In this review, outlines of these studies are described.
GraphscoreDTA: optimized graph neural network for protein–ligand binding affinity prediction
Authors: Wang Kaili , Zhou Renyi , Tang Jing , Li Min
Published: May 2023

Motivation

Computational approaches for identifying the protein–ligand binding affinity can greatly facilitate drug discovery and development. At present, many deep learning-based models are proposed to predict the protein–ligand binding affinity and achieve significant performance improvement. However, protein–ligand binding affinity prediction still has fundamental challenges. One challenge is that the mutual information between proteins and ligands is hard to capture. Another challenge is how to find and highlight the important atoms of the ligands and residues of the proteins.

Results

To solve these limitations, we develop a novel graph neural network strategy with the Vina distance optimization terms (GraphscoreDTA) for predicting protein–ligand binding affinity, which takes the combination of graph neural network, bitransport information mechanism and physics-based distance terms into account for the first time. Unlike other methods, GraphscoreDTA can not only effectively capture the protein–ligand pairs’ mutual information but also highlight the important atoms of the ligands and residues of the proteins. The results show that GraphscoreDTA significantly outperforms existing methods on multiple test sets. Furthermore, the tests of drug–target selectivity on the cyclin-dependent kinase and the homologous protein families demonstrate that GraphscoreDTA is a reliable tool for protein–ligand binding affinity prediction.

Availability and implementation

The resource codes are available at https://github.com/CSUBioGroup/GraphscoreDTA.
Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism
Authors: Li Yaya , Li Chang , Fu Yangfei , Wang Ruixian , Yang Yang , Zhang Man , Zhang Youyi , Wang Xiangbing , Wang Guanlin , Jiang Huan , Zou Yushan , Hu Jiabao , Guo Chunyang , Wang Yajun
Published: May 2023

Insulin-like growth factor 1 (Igf1) is known to promote ovarian maturation by interacting with other hormones. However, the limited research on the role of Igf1 in the energy metabolism supply of gonads has hindered further exploration. To explore the role of Igf1 in gonadal development of silver pomfret, we analyzed the expression levels and the localization of igf1 mRNA and protein during testicular and ovarian development of silver pomfret. The results of the study showed upregulation of Igf1 in the critical period of vitellogenesis and sperm meiosis, which was found to be mainly expressed in the somatic cells of the gonads. Upon adding E2 and Igf1 to cultured gonadal tissues, the expression of energy-related genes was significantly increased, along with the E2-enhanced effect of Igf1 in the testis. Importantly, stimulation of both ovaries and testes with E2 and Igf1 led to a remarkable increase in the expression of vitellogenesis and meiosis-related genes. Therefore, we conclude that Igf1 promotes vitellogenesis and sperm meiosis by regulating gonadal energy production. Moreover, the expression of Igf1 in gonads is significantly regulated by E2. These findings provide new insights for the research of Igf1 in fish breeding, thus allowing the regulation of energy metabolism between growth and reproduction for successful reproductive outcomes.
One-step targeted maximum likelihood estimation for targeting cause-specific absolute risks and survival curves
Authors: Rytgaard H C W , van der Laan M J
Published: May 2023

This paper considers the one-step targeted maximum likelihood estimation methodology for multi-dimensional causal parameters in general survival and competing risk settings where event times take place on the positive real line and are subject to right censoring. We focus on effects of baseline treatment decisions possibly confounded by pretreatment covariates, but remark that our work generalizes to settings with time-varying treatment regimes and time-dependent confounding. We point out two overall contributions of our work. First, our methods can be used to obtain simultaneous inference for treatment effects on multiple absolute risks in competing risk settings. Second, our methods can be used to achieve inference for the full survival curve, or a full absolute risk curve, across time. The one-step targeted maximum likelihood procedure is based on a one-dimensional universal least favourable submodel for each cause-specific hazard that we implement in recursive steps along a corresponding nonuniversal multivariate least favourable submodel. Our empirical study demonstrates the practical use of the methods.
Familiarity with Evidence-Based Practice and Self-Evaluated Service Quality: The Mediating Roles of Preference for Authority-Based Evidence and Professional Autonomy
Authors: Li Xiao , Tan Ling , Sun Xiaoyue
Published: May 2023

Evidence-based practice (EBP) is a model that integrates research, education and practice. Although it has been generally promoted and applied in various practice settings, few studies have examined whether EBP is related to service quality in social work. In this study, a sample of 2,987 licensed social workers was drawn from the Chinese Social Workers Survey, and a model that connects familiarity with the EBP process and service quality was tested. Additionally, this study examined the influences of anti-authority culture and professional autonomy on self-perceived service quality. Results showed that the preference for authority-based evidence is prevalent amongst the social workers in this sample. Familiarity with the EBP process is positively related to self-evaluated service quality. Preference for authority-based evidence and professional autonomy partially mediates the relationship between familiarity with the EBP and self-evaluated service quality. These findings provide insights into the EBP process amongst Chinese social workers. Educational implications include promoting high-quality evidence and emphasising critical thinking and accountability in EBP education.

Summary

This study drew a sample of 2987 licensed social workers from the Chinese Social Workers Survey and tested a model that connects familiarity with EBP process and self-evaluated service quality. The findings revealed that the respondents who are more familiar with the EBP process are more likely to report better service quality Chinese social workers' self-reported service quality is partially built on the professional autonomy and anti-authority culture promoted by the EBP movement.
Digitalisation of Indian smart cities: post-Covid-19 approaches to data, recognition and health monitoring
Authors: Krishnan Sneha
Published: May 2023

There is a proliferation of digitalisation of urban and health services in India under the Smart City and Digital Health missions, respectively. This study brings digital and feminist geographies together to understand the role of technologies in urban areas, particularly in health service delivery and how healthcare workers mediate these health platforms. Using a case study of Varanasi city in Uttar Pradesh, India this study documents whether—and to what extent—digital technologies and services enable citizens and service providers to access and improve their lived experiences. The findings indicate a top-down, innovation-focussed model is adopted which excludes and alienates different user groups and citizens shaping their interaction and access to these services.
Assessing the Quality of Metagenomic Next-Generation Sequencing for Pathogen Detection in Lower Respiratory Infections
Authors: Diao Zhenli , Zhang Yuanfeng , Chen Yuqing , Han Yanxi , Chang Lu , Ma Yu , Feng Lei , Huang Tao , Zhang Rui , Li Jinming
Published: May 2023

Background

Laboratory-developed metagenomic next-generation sequencing (mNGS) assays are increasingly being used for the diagnosis of infectious disease. To ensure comparable results and advance the quality control for the mNGS assay, we initiated a large-scale multicenter quality assessment to scrutinize the ability of mNGS to detect pathogens in lower respiratory infections.

Methods

A reference panel containing artificial microbial communities and real clinical samples was used to assess the performance of 122 laboratories. We comprehensively evaluated the reliability, the source of false-positive and false-negative microbes, as well as the ability to interpret the results.

Results

A wide variety of weighted F1-scores was observed across 122 participants, with a range from 0.20 to 0.97. The majority of false positive microbes (68.56%, 399/582) were introduced from “wet lab.” The loss of microbial sequence during wet labs was the chief cause (76.18%, 275/361) of false-negative errors. When the human context is 2 × 105 copies/mL, most DNA and RNA viruses at titers above 104 copies/mL could be detected by >80% of the participants, while >90% of the laboratories could detect bacteria and fungi at titers lower than 103 copies/mL. A total of 10.66% (13/122) to 38.52% (47/122) of the participants could detect the target pathogens but failed to reach a correct etiological diagnosis.

Conclusions

This study clarified the sources of false-positive and false-negative results and evaluated the performance of interpreting the results. This study was valuable for clinical mNGS laboratories to improve method development, avoid erroneous results being reported, and implement regulatory quality controls in the clinic.
Relationship between remnant cholesterol and risk of heart failure in participants with diabetes mellitus
Authors: Wang Ruoting , Gerstein Hertzel C , Van Spall Harriette G C , Lip Gregory Y H , Olier Ivan , Ortega-Martorell Sandra , Thabane Lehana , Ye Zebing , Li Guowei
Published: May 2023

Background

Evidence about the association between calculated remnant cholesterol (RC) and risk of heart failure (HF) in participants with diabetes mellitus (DM) remains sparse and limited.

Methods

We included a total of 22 230 participants with DM from the UK Biobank for analyses. Participants were categorized into three groups based on their baseline RC measures: low (with a mean RC of 0.41 mmol/L), moderate (0.66 mmol/L), and high (1.04 mmol/L). Cox proportional hazards models were used to evaluate the relationship between RC groups and HF risk. We performed discordance analysis to evaluate whether RC was associated with HF risk independently of low-density lipoprotein cholesterol (LDL-C).

Results

During a mean follow-up period of 11.5 years, there were a total of 2232 HF events observed. The moderate RC group was significantly related with a 15% increased risk of HF when compared with low RC group (hazard ratio [HR] = 1.15, 95% confidence interval [CI]: 1.01—1.32), while the high RC group with a 23% higher HF risk (HR = 1.23, 95% CI: 1.05–1.43). There was significant relationship between RC as a continuous measure and the increased HF risk (P < 0.01). The association between RC and risk of HF was stronger in participants with HbA1c level ≥ 53 mmol/mol when compared with HbA1c < 53 mmol/mol (P for interaction = 0.02). Results from discordance analyses showed that RC was significantly related to HF risk independent of LDL-C measures.

Conclusions

Elevated RC was significantly associated with risk of HF in patients with DM. Moreover, RC was significantly related to HF risk independent of LDL-C measures. These findings may highlight the importance of RC management to HF risk in patients with DM.
An estimation of the consequences of reinforcing the 2016 and 2019 European Society of Cardiology/European Atherosclerosis Society guidelines on current lipid-lowering treatment in patients with type 2 diabetes in tertiary care—a SwissDiab study
Authors: Singeisen Hélène , Renström Frida , Laimer Markus , Lehmann Roger , Bilz Stefan , Brändle Michael
Published: May 2023

Aims

In 2019, the European Society of Cardiology/European Atherosclerosis Society updated the 2016 guidelines for the management of dyslipidaemias recommending more stringent low-density lipoprotein cholesterol (LDL-C) targets in diabetes mellitus type 2 (DM2). Based on a real-world patient population, this study aimed to determine the feasibility and cost of attaining guideline-recommended LDL-C targets, and assess cardiovascular benefit.

Methods and results

The Swiss Diabetes Registry is a multicentre longitudinal observational study of outpatients in tertiary diabetes care. Patients with DM2 and a visit between 1 January 2018 and 31 August 2019 that failed the 2016 LDL-C target were identified. The theoretical intensification of current lipid-lowering medication needed to reach the 2016 and 2019 LDL-C target was determined and the cost thereof extrapolated. The expected number of major adverse cardiovascular events (MACE) prevented by treatment intensification was estimated. Two hundred and ninety-four patients (74.8%) failed the 2016 LDL-C target. The percentage of patients that theoretically achieved the 2016 and 2019 target with the indicated treatment modifications were high-intensity statin, 21.4% and 13.3%; ezetimibe, 46.6% and 27.9%; proprotein convertase subtilisin/kexin type 9 inhibitor (PCSK9i), 30.6% and 53.7%; ezetimibe and PCSK9i, 1.0% and 3.1%; whereas one (0.3%) and five patients (1.7%) failed to reach target, respectively. Achieving the 2016 vs. 2019 target would reduce the estimated 4-year MACE from 24.9 to 18.6 vs. 17.4 events, at an additional annual cost of medication of 2140 Swiss francs (CHF) vs. 3681 CHF per patient, respectively.

Conclusions

For 68% of the patients, intensifying statin treatment and/or adding ezetimibe would be sufficient to reach the 2016 target, whereas 57% would require cost-intensive PCSK9i therapy to reach the 2019 target, with limited additional medium-term cardiovascular benefit.
Temporal patterns of antibiotic prescribing for sore throat, otitis media, and sinusitis: a longitudinal study of general practitioner registrars
Authors: Turner Alexandria , van Driel Mieke L , Mitchell Benjamin L , Davis Joshua S , Fielding Alison , Davey Andrew , Holliday Elizabeth , Ball Jean , Ralston Anna , Tapley Amanda , Mulquiney Katie , Baillie Emma J , Spike Neil , Clarke Lisa , Magin Parker
Published: May 2023

Background

Antibiotics provide minimal benefit for sore throat, otitis media, and sinusitis. Antibiotic stewardship, with reduced prescribing, is required to address antibiotic resistance. As most antibiotic prescribing occurs in general practice and prescribing habits develop early, general practitioner (GP) trainees (registrars) are important for effective antibiotic stewardship.

Objectives

To establish temporal trends in Australian registrars’ antibiotic prescribing for acute sore throat, acute otitis media, and acute sinusitis.

Design

A longitudinal analysis of data from the Registrar Clinical Encounters in Training (ReCEnT) study from 2010 to 2019.

Participants

ReCEnT is an ongoing cohort study of registrars’ in-consultation experiences and clinical behaviours. Pre-2016, 5 of 17 Australian training regions participated. From 2016, 3 of 9 regions (42% of Australian registrars) participate.

Main measures

The outcome was prescription of an antibiotic for a new acute problem/diagnosis of sore throat, otitis media, or sinusitis. The study factor was year (2010–2019).

Key results

Antibiotics were prescribed in 66% of sore throat diagnoses, 81% of otitis media, and in 72% of sinusitis. Prescribing frequencies decreased between 2010 and 2019 by 16% for sore throat (from 76% to 60%) by 11% for otitis media (from 88% to 77%) and by 18% for sinusitis (from 84% to 66%). In multivariable analyses, “Year” was associated with reduced prescribing for sore throat (OR 0.89; 95%CI 0.86–0.92; p < 0.001), otitis media (OR 0.90; 95%CI 0.86–0.94; p < 0.001), and sinusitis (OR 0.90; 95%CI 0.86, 0.94; p < 0.001).

Conclusions

Registrars’ prescribing rates for sore throat, otitis media, and sinusitis significantly decreased during the period 2010–2019. However, educational (and other) interventions to further reduce prescribing are warranted.
Effect of Different Types of Sequence Data on Palaeognath Phylogeny
Authors: Takezaki Naoko
Published: May 2023

Palaeognathae consists of five groups of extant species: flighted tinamous (1) and four flightless groups: kiwi (2), cassowaries and emu (3), rheas (4), and ostriches (5). Molecular studies supported the groupings of extinct moas with tinamous and elephant birds with kiwi as well as ostriches as the group that diverged first among the five groups. However, phylogenetic relationships among the five groups are still controversial. Previous studies showed extensive heterogeneity in estimated gene tree topologies from conserved nonexonic elements, introns, and ultraconserved elements. Using the noncoding loci together with protein-coding loci, this study investigated the factors that affected gene tree estimation error and the relationships among the five groups. Using closely related ostrich rather than distantly related chicken as the outgroup, concatenated and gene tree–based approaches supported rheas as the group that diverged first among groups (1)–(4). Whereas gene tree estimation error increased using loci with low sequence divergence and short length, topological bias in estimated trees occurred using loci with high sequence divergence and/or nucleotide composition bias and heterogeneity, which more occurred in trees estimated from coding loci than noncoding loci. Regarding the relationships of (1)–(4), the site patterns by parsimony criterion appeared less susceptible to the bias than tree construction assuming stationary time-homogeneous model and suggested the clustering of kiwi and cassowaries and emu the most likely with ∼40% support rather than the clustering of kiwi and rheas and that of kiwi and tinamous with 30% support each.
Pilot study of an arts- and theatre-based HIV prevention intervention for men who have sex with men and transgender women migrants in South Africa: acceptability, feasibility and preliminary efficacy
Authors: Alessi E J , Lee Y G , Chikalogwe V P , Tarusarira W , Raymond H , Lynn M , Kahn S
Published: May 2023

Innovative approaches addressing the elevated human immunodeficiency virus (HIV) risk among men who have sex with men (MSM) or transgender women (TGW) migrants in South Africa are urgently needed. We sought to present the acceptability, feasibility and preliminary efficacy of ‘Externalize and Mobilize!’, a multi-session arts- and theatre-based HIV prevention group intervention for MSM and TGW migrants in South Africa. Fourteen participants—MSM (n = 7; 50%), genderqueer/nonbinary persons (n = 4; 29%) and TGW (n = 3; 21%)—in Cape Town were recruited and enrolled in the intervention and administered pre- and post-intervention assessments of HIV knowledge, HIV risk-reduction self-efficacy, stigma and resilience. The intervention, delivered over 4 days, was completed by all 14 participants. Scores on HIV knowledge and HIV risk-reduction self-efficacy were statistically significantly higher at post-intervention compared with pre-intervention. Additionally, participants responded affirmatively (i.e. ‘Agree’ or ‘Strongly agree’) on all items assessing intervention acceptability. Findings demonstrate the high acceptability, feasibility and preliminary efficacy of an arts- and theatre-based intervention for increasing HIV knowledge and HIV risk-reduction self-efficacy among MSM and TGW migrants in South Africa. This study provides further support for the use of creative and innovative interventions to address entrenched HIV disparities in South Africa.
Paired passive acoustic and gillnet sampling reveal the utility of bioacoustics for monitoring fish populations in a turbid estuary
Authors: Souza Jr P M , Olsen Z , Brandl S J
Published: May 2023

Estuaries worldwide provide critical habitat to many ecologically and socioeconomically important fish species. However, the high turbidity and strong environmental gradients inherent to many estuaries make the monitoring of fish populations a challenging task. To assess the health and diversity of local fish populations, resource managers often employ fisheries-independent sampling methods, which result in valuable population assessments, but suffer from low-temporal resolution and high rates of mortality. Passive acoustic monitoring (PAM) provides a near-continuous, non-invasive means for monitoring soniferous fish activity over extended periods, but its utility for quantifying entire fish communities is largely untested, especially in turbid estuaries. Here, we use synchronized acoustic and gillnet sampling at 51 locations in the Mission-Aransas Estuary (TX), across two sampling seasons to test correlations between acoustic indices and gillnet catch. Positive correlations were observed between gillnet catch and sound pressure levels in both broadband (50–22050 Hz) and low-frequency (50–2500 Hz) bands. Additionally, sciaenid calling activity was positively correlated with sciaenid catch during the spring, when most spawning activity occurs. Our results indicate that PAM can be used to monitor the relative abundances of fish in Gulf of Mexico estuaries and beyond, although this is subject to species identity and seasonal dynamics.
Wheat or Chaff? A Compound Selection Model Based on Look-Up Data
Authors: Paulsen Mikkel Ekeland
Published: May 2023

Which compounds should be included in general-purpose dictionaries is often an open question that is answered with a case-by-case consideration of all compounds above a certain corpus frequency threshold. Another way to determine which compounds should be listed, is to examine which compounds, or rather which compound properties, are in demand by the users. This study uses look-up data from the two officially sanctioned, general-purpose dictionaries of Norwegian (Bokmålsordboka and Nynorskordboka) to derive an explicit compound selection model that performs with comparable sensitivity and specificity as the traditional procedure. These findings demonstrate that it is indeed possible to arrive at a fully operational and explicit compound selection model that meets the needs of users. With such a tool at their disposal, lexicographers would be able to separate the wheat from the chaff in the boundless field that is the compound lexicon of North Germanic Languages.
The Burden of COVID-19 in the Immunocompromised Patient: Implications for Vaccination and Needs for the Future
Authors: Antinori Andrea , Bausch-Jurken Mary
Published: May 2023

Approximately 3% of US adults are immunocompromised and less capable of fighting infections such as SARS-CoV-2 (the causative agent of COVID-19). Individuals may be immunocompromised for reasons related to an underlying medical condition or to immunomodulatory therapies that alter the immune response. In general, vaccination with mRNA–based vaccines is effective at reducing COVID-19–associated hospitalization and death among immunocompromised populations, particularly after 3 or more doses. However, the immunocompromised population is heterogeneous, with COVID-19 vaccine-elicited immune responses and risk for severe COVID-19 existing on a continuum. Therefore, understanding the impact of vaccination and the complexity of immune responses across heterogeneous immunocompromised individuals is essential for guiding effective vaccination regimens including additional (booster) doses. In this article, we provide an overview of the immunocompromised population and the burden of disease attributable to COVID-19, while discussing key opportunities and challenges of vaccinating immunocompromised individuals.
COVID-19 pandemic and the quality of antibiotic use in primary care: an interrupted time-series study
Authors: Domingues Mariana , Torre Carla , Guerreiro José Pedro , Barata Pedro , Correia-Neves Margarida , Rocha João , Sepodes Bruno , Teixeira Rodrigues António
Published: May 2023

The coronavirus disease-19 pandemic and the related public health mitigation measures have impacted the transmission of infectious diseases; however, their impact on the use of antibacterials has not yet been extensively evaluated. This study evaluated the impact of the pandemic on the consumption patterns of antibacterials for systemic use in primary care in Portugal. An interrupted time-series analysis was performed using the autoregressive integrated moving average model of the antibacterials dispensed in the community pharmacies in Portugal from 1 January 2016 to 30 June 2022. Monthly rates of absolute consumption (all antibacterials for systemic use, and specifically penicillins; cephalosporins; macrolides, lincosamides, and streptogramins; and quinolones) and the relative consumption of antibacterials (penicillins sensitive to β-lactamase, penicillin combinations including β-lactamase inhibitors, third- and fourth-generation cephalosporins, fluoroquinolones, and the ratio of broad- to narrow-spectrum antibacterials) were estimated. Antibiotic consumption was expressed in defined daily doses per 1000 inhabitants per day (DID). In Portugal, the consumption of antibacterials (J01) declined sharply immediately after the beginning of the pandemic, having a significant reduction of >5 DID (P < .0001). A similar, short-term impact was found for penicillins (−2.920 DID; P < .0001); cephalosporins (−0.428 DID; P < .0001); macrolides, lincosamides, and streptogramins (−0.681 DID; P = .0021); and quinolones (−0.320 DID; P < .0001). A long-term increase was found for cephalosporins (+0.019 DID per month; P < .0001). Relative consumption changes were only found for third- and fourth-generation cephalosporins (0.0734%). Our study suggests that the coronavirus disease-19 pandemic may have resulted in a decrease in antibiotic use, with no significant changes in the relative dispense. Uncertainties regarding the long-term effects of the pandemic and its impact on the rates of resistance remain.
Training for the United Nations in the Twenty-First Century; Professionalism Training on Leadership, Negotiation, and Gender for Model United Nations Simulations
Authors: O'Dell Roni Kay M , Scott Ariana B , Nealon Mark J , Franzino Brianna N
Published: May 2023

The world needs people who understand the crucial importance of global governance. Training students to participate in a Model United Nations (MUN) simulation provides them with the critical thinking, negotiation, and diplomacy skills that prepare them to work in international relations positions and address negotiation and diplomacy challenges. This article provides a fresh look at why MUN is important by analyzing MUN training guides used to teach students how to participate in MUN simulations. It finds that the training guides offer excellent insight into the functions and structure of the United Nations, but little in the form of learning how to negotiate or engage in diplomacy. Specifically missing are discussions of leadership, negotiation from interest, not position (also known as principled negotiation), and how to train students to be inclusive and respond to discrimination or bias during negotiations. The article includes a description of active learning and learner-centered teaching techniques and provides insight into how a specific MUN team implemented professionalism training in the 2010s. The article is written in collaboration between an MUN advisor and students who trained as MUN delegates, integrating the voices and perspectives of those who train and those who are trained.
Kappa Free Light Chain Drift Prompts the Need for a New Upper Limit of Normal Free Light Chain Ratio to Avoid an Epidemic of Kappa Light Chain Monoclonal Gammopathy of Undermined Significance
Authors: Rozenova Krasimira , Willrich Maria , Snyder Melissa , Dasari Surendra , Kourelis Taxiarchis , Rajkumar S Vincent , Kumar Shaji , Dispenzieri Angela , Murray David L
Published: May 2023

Background

Multiple laboratory tests are employed for detection of monoclonal proteins in patients and include serum protein electrophoresis (SPEP), immunofixation electrophoresis, free light chain (FLC) immunoassay, and mass spectrometry (Mass-Fix). Recently, reports on a drift in FLC quantitation results have been brought to light.

Methods

We studied a cohort of 16 887 patients whose sera were tested for a monoclonal protein by a FLC assay, serum protein electrophoresis, and Mass-Fix. This is a retrospective study designed to assess the impact of a drift on the performance of FLC ratio (rFLC) in groups of patients with and without detectable plasma cell disorders (PCDs).

Results

The results demonstrated that 63% of patients with monoclonal protein equal or higher than 2 g/L (by SPEP) had an abnormal rFLC (reference range 0.26–1.65). Conversely, 16% of patients with undetectable monoclonal protein by other methods (i.e., SPEP and Mass-Fix) who also had no record of treated PCD had an abnormal rFLC. In these cases, there was an imbalance in the number of kappa high rFLCs to lambda low rFLCs of 201 to 1.

Conclusions

The results of this study suggest decreased specificity of rFLC for a monoclonal kappa FLC in the 1.65 to 3.0 range.
The Role of Burn Centers in the Treatment of Necrotizing Soft-Tissue Infections: A Nationwide Dutch Study
Authors: Suijker Jaco , Wurfbain Lisca , Emmen Anouk M L H , Pijpe Anouk , Kwa Kelly A A , van der Vlies Cornelis H , Nieuwenhuis Marianne K , van Zuijlen Paul P M , Meij-de Vries Annebeth
Published: May 2023

Patients with extensive and complex wounds due to Necrotizing Soft-Tissue Infections (NSTI) may be referred to a burn center. This study describes the characteristics, outcomes, as well as diagnostic challenges of these patients. Patients admitted to three hospitals with a burn center for the treatment of NSTI in a 5-year period were included. Eighty patients (median age 54 years, 60% male) were identified, of whom 30 (38%) were referred by other centers, usually after survival of the initial septic phase. Those referred from other centers, compared to those primarily admitted to the study hospitals, were more likely to have group A streptococcal involvement (62% vs 35%, p = .02), larger wounds (median 7% vs 2% total body surface area, p < .001), and a longer length of stay (median 49 vs 22 days, p < .001). Despite a high incidence of septic shock (50%), the mortality rate was low (12%) for those primarily admitted. Approximately half (53%) of the patients were initially misdiagnosed upon presentation, which was associated with delay to first surgery (16 hours vs 4 hours, p < .001). Those initially misdiagnosed had more (severe) comorbidities, and less frequently reported pain or blue livid discoloration of the skin. This study underlines the burn centers’ function as referral centers for extensively affected patients with NSTI. Besides the unique wound and reconstructive expertise, the low mortality rate indicates these centers provide adequate acute care as well. A major remaining challenge remains recognition of the disease upon presentation. Future studies in which factors associated with misdiagnosis are explored are needed.
Just Transition as an Evolving Concept in International Climate Law
Authors: Johansson Vilja
Published: May 2023

‘Just transition’ has grown into an increasingly popular concept in climate policy. During the recent decade, it has been included in both international and national climate law frameworks. The concept, however, has not received much attention from legal scholars. Addressing this gap, this article analyses the meaning and legal implications of just transition, specifically within international climate law. Against this backdrop, it shows that, following the Paris Agreement, just transition has evolved into an increasingly important concept in how climate law principles and obligations are interpreted and developed. It further highlights a substantial evolution of just transition from a labour-centred to a more comprehensive concept that helps underline the importance of implementing climate measures in a way that engages and protects affected and vulnerable people and communities.
Long-chain polyphosphates inhibit type I interferon signaling and augment LPS-induced cytokine secretion in human leukocytes
Authors: Pirttiniemi Anniina , Adeshara Krishna , Happonen Natalie , Einarsdottir Elisabet , Katayama Shintaro , Salmenkari Hanne , Hörkkö Sohvi , Kere Juha , Groop Per-Henrik , Lehto Markku
Published: May 2023

Inorganic polyphosphates are evolutionarily conserved bioactive phosphate polymers found as various chain lengths in all living organisms. In mammals, polyphosphates play a vital role in the regulation of cellular metabolism, coagulation, and inflammation. Long-chain polyphosphates are found along with endotoxins in pathogenic gram-negative bacteria and can participate in bacterial virulence. We aimed to investigate whether exogenously administered polyphosphates modulate human leukocyte function in vitro by treating the cells with 3 different chain lengths of polyphosphates (P14, P100, and P700). The long-chain polyphosphates, P700, had a remarkable capacity to downregulate type I interferon signaling dose dependently in THP1-Dual cells while only a slight elevation could be observed in the NF-κB pathway with the highest dose of P700. P700 treatment decreased lipopolysaccharide-induced IFNβ transcription and secretion, reduced STAT1 phosphorylation, and downregulated subsequent interferon-stimulated gene expression in primary human peripheral blood mononuclear cells. P700 also augmented lipopolysaccharide-induced secretion of IL-1α, IL-1β, IL-4, IL-5, IL-10, and IFNγ. Furthermore, P700 has previously been reported to increase the phosphorylation of several intracellular signaling mediators, such as AKT, mTOR, ERK, p38, GSK3α/β, HSP27, and JNK pathway components, which was supported by our findings. Taken together, these observations demonstrate the extensive modulatory effects P700 has on cytokine signaling and the inhibitory effects specifically targeted to type I interferon signaling in human leukocytes.
Low IL-13Rα1 expression on mast cells tunes them unresponsive to IL-13
Authors: Salomaa Tanja , Kummola Laura , González-Rodríguez Martín Ignacio , Hiihtola Lotta , Järvinen Tero A H , Junttila Ilkka S
Published: May 2023

Cytokine-mediated mast cell regulation enables precise optimization of their own proinflammatory cytokine production. During allergic inflammation, interleukin (IL)-4 regulates mast cell functions, tissue homing, and proliferation, but the direct role of closely related IL-13 for mast cell activation remains unclear. Previous work has shown that mast cells are potent IL-13 producers, but here we show that mouse mast cells do not directly respond to IL-13 by Stat6 activation, as they do not express measurable amount of IL-13 receptor α1 (IL-4Rα1) messenger RNA. Consequently, IL-4 responses are mediated via type I IL-4R (IL-4/IL4Rα/γC), and IL-4–induced Stat6 activation is abolished in γC-deficient mast cells. Type II IL-4R deficiency (IL-13Rα1 knockout) has no effect on IL-4–induced Stat6 activation. In basophils, both IL-4 and IL-13 induce Stat6 activation in wild-type and γC-deficient cells, while in type II IL-4R–deficient basophils, IL-4 signaling is impaired at low ligand concentration. Thus, mast cell and basophil sensitivity to IL-4/IL-13 is different, and in mast cells, lack of IL-13Rα1 expression likely explains their unresponsiveness to IL-13.
Structural and electronic characterization of fluorine-doped La0.5Sr0.5CoO3−δ using electron energy-loss spectroscopy
Authors: Aso Ryotaro , Katsumata Takuya , Nakamura Takashi , Watase Yusuke , Amezawa Koji , Murakami Yasukazu
Published: May 2023

Perovskite oxides, ABO3, are potential catalysts for the oxygen evolution reaction, which is important in the production of hydrogen as a sustainable energy resource. Optimizing the chemical composition of such oxides by substitution or doping with additional elements is an effective approach to improving the activity of such catalysts. Here, we characterized the crystal and electronic structures of fluorine-doped La0.5Sr0.5CoO3−δ particles using scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy (EELS). High-resolution STEM imaging demonstrated the formation of a disordered surface phase caused by fluorine doping. In addition, spatially resolved EELS data showed that fluorine anions were introduced into the interiors of the particles and that Co ions near the surfaces were slightly reduced by fluorine doping in conjunction with the loss of oxygen ions. Peak fitting of energy-loss near-edge structure data demonstrated an unexpected nanostructure in the vicinity of the surface. An EELS characterization comprising elemental mapping together with an energy-loss near-edge structure analysis indicated that this nanostructure could not be assigned to Co-based materials but rather to the solid electrolyte BaF2. Complementary structural and electronic characterizations using STEM and EELS as demonstrated herein evidently have the potential to play an increasingly important role in elucidating the nanostructures of functional materials.