bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Cu2O Substrate on Photoinduced Hydrophilicity of TiO2 and ZnO Nanocoatings
Authors: Maevskaya Maria V. , Rudakova Aida V. , Emeline Alexei V. , Bahnemann Detlef W.
Published: June 2021

The effect of a Cu2O substrate on the photoinduced alteration of the hydrophilicity of TiO2 and ZnO surfaces was studied. It was demonstrated that the formation of heterostructures Cu2O/TiO2 and Cu2O/ZnO strongly changed the direction of the photoinduced alteration of surface hydrophilicity: while both TiO2 and ZnO demonstrate surface transition to superhydrophilic state under UV irradiation and no significant alteration of the surface hydrophilicity under visible light irradiation, the formation of Cu2O/TiO2 and Cu2O/ZnO heterostructures resulted in photoinduced decay of the surface hydrophilicity caused by both UV and visible light irradiation. All observed photoinduced changes of the surface hydrophilicity were compared and analyzed in terms of photoinduced alteration of the surface free energy and its polar and dispersive components. Alteration of the photoinduced hydrophilic behavior of TiO2 and ZnO surfaces caused by formation of the corresponding heterostructures with Cu2O are explained within the mechanism of electron transfer and increasing of the electron concentration on the TiO2 and ZnO surfaces.
Fasting and Exercise Induce Changes in Serum Vitamin D Metabolites in Healthy Men
Authors: Żychowska Małgorzata , Rola Rafał , Borkowska Andżelika , Tomczyk Maja , Kortas Jakub , Anczykowska Katarzyna , Pilis Karol , Kowalski Konrad , Pilch Wanda , Antosiewicz Jędrzej
Published: June 2021

Background: Vitamin D plays pleiotropic roles in the body and hence, changes in its metabolism and distribution during starvation could play an important role in the adaptive response to famine. We aimed to identify the responses of some vitamin D metabolites to 8 d of fasting and exercise. Methods: A repeated-measures design was implemented, in which 14 male volunteers fasted for 8 d and performed an exercise test before and after fasting. Serum samples were collected on day 1 after night fasting and after 8 d of complete food restriction, before and 1 h and 3 h after exercise. Results: After 8 d of fasting, compared with baseline values, serum 24,25(OH)2D3 and 3-epi-25(OH)D3 levels significantly increased; those of 25(OH)D3 and 1,25(OH)2D3 were unaffected; and those of 25(OH)D2 decreased. Exercise on the first day of fasting induced an increase in serum 3-epi-25(OH)D3 levels, while exercise performed after 8 d of fasting induced an increase in 25(OH)D3, 24,25(OH)2D3, 25(OH)D2, and 3-epi-25(OH)D3 levels. Conclusion: Increases in 24,25(OH)2D3 and 3-epi-25(OH)D3 levels imply that fasting stimulates vitamin D metabolism. The effects of exercise on serum vitamin D metabolites, which are most pronounced after fasting and in subjects with serum 25(OH)D3 above 25 ng/mL, support the notion that fasting and exercise augment vitamin D metabolism.
Prenatal Exposure to BPA: The Effects on Hepatic Lipid Metabolism in Male and Female Rat Fetuses
Authors: Tonini Claudia , Segatto Marco , Bertoli Simona , Leone Alessandro , Mazzoli Arianna , Cigliano Luisa , Barberio Laura , Mandalà Maurizio , Pallottini Valentina
Published: June 2021

Bisphenol A (BPA) is an organic chemical compound widely used for manufacturing plastics. BPA exposure originates principally from the diet, but it can also originate from dermal contact. In over 90% of individuals, including pregnant women, BPA is detectable in several body fluids. The effects of this exposure on the fetus are under active investigation in several research laboratories. The aim of our work was to study the impact of prenatal exposure to BPA in the liver of rat fetuses from a sex-dependent point of view. We particularly investigated the effects of prenatal BPA exposure on hepatic lipids because of their crucial role, not only for the liver, but also for the whole-body functions. Our results demonstrate that the liver of rat fetuses, in utero exposed to a very low dose of BPA (2.5 µg/kg/day), displays significant modulations with regard to proteins involved in cholesterol and fatty acid biosynthesis and trafficking. Moreover, an impact on inflammatory process has been observed. All these effects are dependent on sex, being observable only in female rat fetuses. In conclusion, this work demonstrates that maternal exposure to BPA compromises hepatic lipid metabolism in female offspring, and it also reveals the perspective impact of BPA on human health at doses currently considered safe.
Frequency and Clinical Aspects of Neurological and Psychiatric Symptoms in Patients with Non-Celiac Wheat Sensitivity
Authors: Carroccio Antonio , Soresi Maurizio , Chiavetta Marta , La Blasca Francesco , Compagnoni Stella , Giuliano Alessandra , Fayer Francesca , Mandreucci Francesca , Castellucci Daniele , Seidita Aurelio , Affronti Andrea , Florena Ada Maria , Mansueto Pasquale
Published: June 2021

Background: Non-Celiac Wheat Sensitivity (NCWS) is characterized by both intestinal and extra-intestinal symptoms. The study aims to investigate the frequency of neuropsychiatric manifestations in NCWS patients and identify their clinical and demographic characteristics. Methods: 278 clinical records of NCWS patients, diagnosed by a double-blind placebo-controlled wheat challenge between 2006 and 2020, were retrospectively revised. Fifty-two patients with Celiac Disease (CD) and 54 patients with Irritable Bowel Syndrome (IBS) served as controls. Results: 87% of the NCWS patients had an IBS-like clinical presentation. The NCWS group showed a longer duration of symptoms, a higher frequency of positive serum anti-nuclear antibodies than CD and IBS patients, and a higher frequency of DQ2/DQ8 haplotypes and duodenal mucosa lymphocytosis than IBS controls. In addition, 50% of NCWS patients showed neuropsychiatric manifestations, while lower percentages were observed in CD (25%) and IBS (28%) controls. Neuropsychiatric symptoms in NCWS were more frequently associated with the male sex, longer duration of symptoms, and IBS-diarrhea-like clinical presentation. Conclusions: Our data suggest that in patients with IBS-like symptoms and neuropsychiatric manifestations of unknown cause, it could be useful to investigate a correlation of these symptoms with wheat ingestion to identify NCWS patients with this ‘atypical’ manifestation.
Inflammation and the Association of Vitamin D and Depressive Symptomatology
Authors: Dogan-Sander Ezgi , Mergl Roland , Willenberg Anja , Baber Ronny , Wirkner Kerstin , Riedel-Heller Steffi G. , Röhr Susanne , Schmidt Frank M. , Schomerus Georg , Sander Christian
Published: June 2021

Depression and vitamin D deficiency are major public health problems. The existing literature indicates the complex relationship between depression and vitamin D. The purpose of this study was to examine whether this relationship is moderated or mediated by inflammation. A community sample (n = 7162) from the LIFE-Adult-Study was investigated, for whom depressive symptoms were assessed via the German version of CES-D scale and serum 25-hydroxyvitamin D (25(OH)D) levels and inflammatory markers (IL-6 and CRP levels, WBC count) were quantified. Mediation analyses were performed using Hayes’ PROCESS macro and regression analyses were conducted to test moderation effects. There was a significant negative correlation between CES-D and 25(OH)D, and positive associations between inflammatory markers and CES-D scores. Only WBC partially mediated the association between 25(OH)D levels and depressive symptoms both in a simple mediation model (ab: −0.0042) and a model including covariates (ab: −0.0011). None of the inflammatory markers showed a moderation effect on the association between 25(OH)D levels and depressive symptoms. This present work highlighted the complex relationship between vitamin D, depressive symptoms and inflammation. Future studies are needed to examine the effect of vitamin D supplementation on inflammation and depressive symptomatology for causality assessment.
Subchronic Tolerance Trials of Graded Oral Supplementation with Phenylalanine or Serine in Healthy Adults
Authors: Miura Naoki , Matsumoto Hideki , Cynober Luc , Stover Patrick , Elango Rajavel , Kadowaki Motoni , Bier Dennis , Smriga Miro
Published: June 2021

Phenylalanine and serine are amino acids used in dietary supplements and nutritional products consumed by healthy consumers; however, the safe level of phenylalanine or serine supplementation is unknown. The objective of this study was to conduct two 4-week clinical trials to evaluate the safety and tolerability of graded dosages of oral phenylalanine and oral serine. Healthy male adults (n = 60, 38.2 ± 1.8y) completed graded dosages of either phenylalanine or serine supplement (3, 6, 9 and 12 g/d) for 4 weeks with 2-week wash-out periods in between. Primary outcomes included vitals, a broad spectrum of circulating biochemical analytes, body weight, sleep quality and mental self-assessment. At low dosages, minor changes in serum electrolytes and plasma non-essential amino acids glutamine and aspartic acid concentrations were observed. Serine increased its plasma concentrations at high supplemental dosages (9 and 12 g/day), and phenylalanine increased plasma tyrosine concentrations at 12 g/day, but those changes were not considered toxicologically relevant. No other changes in measured parameters were observed, and study subjects tolerated 4-week-long oral supplementation of phenylalanine or serine without treatment-related adverse events. A clinical, no-observed-adverse-effect-level (NOAEL) of phenylalanine and serine supplementation in healthy adult males was determined to be 12 g/day.
Impact of Portion Control Tools on Portion Size Awareness, Choice and Intake: Systematic Review and Meta-Analysis
Authors: Vargas-Alvarez M. Angeles , Navas-Carretero Santiago , Palla Luigi , Martínez J. Alfredo , Almiron-Roig Eva
Published: June 2021

Portion control utensils and reduced size tableware amongst other tools, have the potential to guide portion size intake but their effectiveness remains controversial. This review evaluated the breadth and effectiveness of existing portion control tools on learning/awareness of appropriate portion sizes (PS), PS choice, and PS consumption. Additional outcomes were energy intake and weight loss. Published records between 2006–2020 (n = 1241) were identified from PubMed and WoS, and 36 publications comparing the impact of portion control tools on awareness (n = 7 studies), selection/choice (n = 14), intake plus related measures (n = 21) and weight status (n = 9) were analyzed. Non-tableware tools included cooking utensils, educational aids and computerized applications. Tableware included mostly reduced-size and portion control/calibrated crockery/cutlery. Overall, 55% of studies reported a significant impact of using a tool (typically smaller bowl, fork or glass; or calibrated plate). A meta-analysis of 28 articles confirmed an overall effect of tool on food intake (d = –0.22; 95%CI: –0.38, –0.06; 21 comparisons), mostly driven by combinations of reduced-size bowls and spoons decreasing serving sizes (d = –0.48; 95%CI: –0.72, –0.24; 8 comparisons) and consumed amounts/energy (d = –0.22; 95%CI: –0.39, –0.05, 9 comparisons), but not by reduced-size plates (d = –0.03; 95%CI: –0.12, 0.06, 7 comparisons). Portion control tools marginally induced weight loss (d = –0.20; 95%CI: –0.37, –0.03; 9 comparisons), especially driven by calibrated tableware. No impact was detected on PS awareness; however, few studies quantified this outcome. Specific portion control tools may be helpful as potentially effective instruments for inclusion as part of weight loss interventions. Reduced size plates per se may not be as effective as previously suggested.
Maize (Zea mays L.): A New Host for Ligustrum witches’ Broom Phytoplasma
Authors: Çağlar Behçet Kemal , Pehlivan Serkan , Atakan Ekrem , Elbeaino Toufic
Published: June 2021

In the 2019–2020 growing season, two corn fields located in İmamoğlu town (Adana Province, Turkey) were surveyed following the appearance of phytoplasma-like symptoms on maize plants. A total of 40 samples were collected and tested in first-round and nested PCR using universal primer pairs P1/P7 and R16F2n/R16R2, respectively. All maize-diseased plants reacted positively, whilst no PCR amplifications were obtained from asymptomatic plants. Blast sequence analysis of R16F2n/R16R2-primed amplicons from different maize isolates showed 99.2% to 100% of identity with the 16S rRNA gene of Ligustrum witches’ broom phytoplasma (LiWBP). To gain additional molecular information on the 16S ribosomal RNA and 23S rRNA intergenic spacer region of LiWBP, not identified previously, the P1/P7-primed amplicons were also sequenced and analyzed. The results show that maize isolates from Turkey share 99.6% to 100% of identity among them, whereas the highest identity found (91%) was with members of groups 16SrII and 16SrXXV (peanut and tea witches’ broom groups, respectively). This distant relationship between LiWBP and members of 16SrII and XXV was also confirmed by RFLP and phylogenetic analyses. This is the first finding of LiWBP on maize in nature, where it was found responsible for phyllody disease of corn plants in Turkey. The additional molecular information acquired in this study on the 16S–23S rRNA intergenic spacer region of LiWBP further corroborates its distant relationship to any other phytoplasma groups.
Recognition Pattern of the Fasciola hepatica Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics
Authors: Becerro-Recio David , González-Miguel Javier , Ucero Alberto , Sotillo Javier , Martínez-Moreno Álvaro , Pérez-Arévalo José , Cwiklinski Krystyna , Dalton John P. , Siles-Lucas Mar
Published: June 2021

Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domain-containing protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica.
Streptococcus Pneumoniae-Associated Hemolytic Uremic Syndrome in the Era of Pneumococcal Vaccine
Authors: Agarwal Hemant , Latifi Samir
Published: June 2021

Streptococcus pneumoniae-associated hemolytic uremic syndrome (Sp-HUS) is a serious complication of invasive pneumococcal disease that is associated with increased mortality in the acute phase and morbidity in the long term. Recently, Sp-HUS definition has undergone revision and cases are categorized as definite, probable, and possible, based on less invasive serological investigations that evaluate Thomsen-Friedenreich crypt antigen (T-antigen) activation. In comparison to the pre-vaccine era, Sp-HUS incidence seems to be decreasing after the introduction of 7-serotype valence and 13-serotype valence pneumococcal vaccines in 2000 and 2010, respectively. However, Sp-HUS cases continue to occur secondary to vaccine failure and emergence of non-vaccine/replacement serotypes. No single hypothesis elucidates the molecular basis for Sp-HUS occurrence, although pneumococcal neuraminidase production and formation of T-antigen antibody complexes on susceptible endothelial and red blood cells continues to remain the most acceptable explanation. Management of Sp-HUS patients remains supportive in nature and better outcomes are being reported secondary to earlier recognition, better diagnostic tools and improved medical care. Recently, the addition of eculizumab therapy in the management of Sp-HUS for control of dysregulated complement activity has demonstrated good outcomes, although randomized clinical trials are awaited. A sustained pneumococcal vaccination program and vigilance for replacement serotypes will be the key for persistent reduction in Sp-HUS cases worldwide.
Benznidazole Treatment: Time- and Dose-Dependence Varies with the Trypanosoma cruzi Strain
Authors: Fonseca Kátia da Silva , Perin Luísa , de Paiva Nívia Carolina Nogueira , da Silva Beatriz Cristiane , Duarte Thays Helena Chaves , Marques Flávia de Souza , Costa Guilherme de Paula , Molina Israel , Correa-Oliveira Rodrigo , Vieira Paula Melo de Abreu , Carneiro Cláudia Martins
Published: June 2021

As the development of new drugs for Chagas disease is not a priority due to its neglected disease status, an option for increasing treatment adherence is to explore alternative treatment regimens, which may decrease the incidence of side effects. Therefore, we evaluated the efficacy of different therapeutic schemes with benznidazole (BNZ) on the acute and chronic phases of the disease, using mice infected with strains that have different BNZ susceptibilities. Our results show that the groups of animals infected by VL-10 strain, when treated in the chronic phase with a lower dose of BNZ for a longer period of time (40 mg/kg/day for 40 days) presented better treatment efficacy than with the standard protocol (100 mg/kg/day for 20 days) although the best result in the treatment of the animals infected by the VL-10 strain was with100 mg/kg/day for 40 days. In the acute infection by the Y and VL-10 strains of T. cruzi, the treatment with a standard dose, but with a longer time of treatment (100 mg/kg/day for 40 days) presented the best results. Given these data, our results indicate that for BNZ, the theory of dose and time proportionality does not apply to the phases of infection.
Safety and Benefits of Contraceptives Implants: A Systematic Review
Authors: Rocca Morena Luigia , Palumbo Anna Rita , Visconti Federica , Di Carlo Costantino
Published: June 2021

Progestin-only contraceptive implants provide long-acting, highly effective reversible contraception. We searched the medical publications in PubMed, CENTRAL, and EMBASE for relevant articles on hormonal implants published in English between 1990 and 2021. Levonorgestrel (LNG) 6-capsule subdermal implants represented the first effective system approved for reversible contraception. The etonogestrel (ENG) single rod dispositive has been widely employed in clinical practice, since it is a highly effective and safe contraceptive method. Abnormal menstrual bleeding is a common ENG side effect, representing the main reason for its premature discontinuation. Emerging evidence demonstrated that it is possible to extend the use of the ENG implant beyond the three-year period for which it is approved. The ENG implant could be an effective and discrete alternative to the IUD in young girls, such as post-partum/post-abortion. Implants should be inserted by trained skilled clinicians who previously provide adequate counselling about their contraceptive effect, benefits, and any possible adverse events. More studies are needed to validate the extended use of the ENG implant for up to 5 years.
Distinct Modulatory Effects of Fever-Range Hyperthermia on the Response of Breast Cancer Cells and Macrophages to Mistletoe (Viscum album L.) Extract
Authors: Kozłowski Henryk M. , Pawlikowska Małgorzata , Sobocińska Justyna , Jędrzejewski Tomasz , Dzialuk Artur , Wrotek Sylwia
Published: June 2021

Heat utility as a critical component of fever is often ignored, although the symptom is observed in many medical conditions. Mistletoe extract (ME) is an adjunctive medication prescribed to cancer patients. The increase in body temperature is frequently observed in patients following ME administration. Nevertheless, the impact of this fever on the effectiveness of therapy is unknown. Therefore, we aimed to investigate the effect of fever-range temperatures on ME-treated breast cancer cells and macrophages. The cells were simultaneously stimulated with ME and subjected to fever-range hyperthermia (FRH; 39 °C or 41 °C). After co-treatment, the cell viability, generation of reactive oxygen species (ROS), cell cycle distribution, and production of pro-inflammatory factors (interleukin (IL)-1β, IL-6, and cyclooxygenase (COX)-2) were evaluated. The results showed that the exposure of ME-treated breast cancer cells to FRH at 39 °C resulted in a slight decrease in their viability, whereas FRH of 41 °C enhanced this effect. Only FRH of 41 °C induced minor changes in ROS level in ME-treated breast cancer cell lines. In ME-treated macrophages, FRH stimulated cell proliferation. The cell cycle distribution analysis showed a difference between cells cultured at 39 °C and 41 °C in all examined cell lines. Moreover, hyperthermia at 41 °C completely inhibited the ME-induced increase in IL-1β and IL-6 expression in MCF-7 breast cancer cells, whereas this effect was not observed in 4T1 breast cancer cells. In contrast, in ME-treated macrophages, FRH of 41 °C strongly up-regulated expression of the pro-inflammatory factors. We conclude that fever is an important component of ME therapy that differentially affects cancer and immune cells.
Comparison of Modern In Vitro Permeability Methods with the Aim of Investigation Nasal Dosage Forms
Authors: Bartos Csilla , Szabó-Révész Piroska , Horváth Tamás , Varga Patrícia , Ambrus Rita
Published: June 2021

Nowadays, the intranasal route has become a reliable alternative route for drug administration to the systemic circulation or central nervous system. However, there are no official in vitro diffusion and dissolution tests especially for the investigation of nasal formulations. Our main goal was to study and compare a well-known and a lesser-known in vitro permeability investigation method, in order to ascertain which was suitable for the determination of drug permeability through the nasal mucosa from different formulations. The vertical diffusion cell (Franz cell) was compared with the horizontal diffusion model (Side-Bi-Side). Raw and nanonized meloxicam containing nasal dosage forms (spray, gel and powder) were tested and compared. It was found that the Side-Bi-Side cell was suitable for the investigation of spray and powder forms. In contrast, the gel was not measurable on the Side-Bi-Side cell; due to its high viscosity, a uniform distribution of the active substance could not be ensured in the donor phase. The Franz cell, designed for the analysis of semi-solid formulations, was desirable for the investigation of nasal gels. It can be concluded that the application of a horizontal cell is recommended for liquid and solid nasal preparations, while the vertical one should be used for semi-solid formulations.
A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization
Authors: Gailliègue Florian N. , Tamošiūnas Mindaugas , André Franck M. , Mir Lluis M.
Published: June 2021

Sonoporation is the process of cell membrane permeabilization, due to exposure to ultrasounds. There is a lack of consensus concerning the mechanisms of sonoporation: Understanding the mechanisms of sonoporation refines the choice of the ultrasonic parameters to be applied on the cells. Cells’ classical exposure systems to ultrasounds have several drawbacks, like the immersion of the cells in large volumes of liquid, the nonhomogeneous acoustic pressure in the large sample, and thus, the necessity for magnetic stirring to somehow homogenize the exposure of the cells. This article reports the development and characterization of a novel system allowing the exposure to ultrasounds of very small volumes and their observation under the microscope. The observation under a microscope imposes the exposure of cells and Giant Unilamellar Vesicles under an oblique incidence, as well as the very unusual presence of rigid walls limiting the sonicated volume. The advantages of this new setup are not only the use of a very small volume of cells culture medium/microbubbles (MB), but the presence of flat walls near the sonicated region that results in a more homogeneous ultrasonic pressure field, and thus, the control of the focal distance and the real exposure time. The setup presented here comprises the ability to survey the geometrical and dynamical aspects of the exposure of cells and MB to ultrasounds, if an ultrafast camera is used. Indeed, the setup thus fulfills all the requirements to apply ultrasounds conveniently, for accurate mechanistic experiments under an inverted fluorescence microscope, and it could have interesting applications in photoacoustic research.
Smart Nanoparticles for Chemo-Based Combinational Therapy
Authors: Shrestha Binita , Wang Lijun , Brey Eric M. , Uribe Gabriela Romero , Tang Liang
Published: June 2021

Cancer is a heterogeneous and complex disease. Traditional cancer therapy is associated with low therapeutic index, acquired resistance, and various adverse effects. With the increasing understanding of cancer biology and technology advancements, more strategies have been exploited to optimize the therapeutic outcomes. The rapid development and application of nanomedicine have motivated this progress. Combinational regimen, for instance, has become an indispensable approach for effective cancer treatment, including the combination of chemotherapeutic agents, chemo-energy, chemo-gene, chemo-small molecules, and chemo-immunology. Additionally, smart nanoplatforms that respond to external stimuli (such as light, temperature, ultrasound, and magnetic field), and/or to internal stimuli (such as changes in pH, enzymes, hypoxia, and redox) have been extensively investigated to improve precision therapy. Smart nanoplatforms for combinational therapy have demonstrated the potential to be the next generation cancer treatment regimen. This review aims to highlight the recent advances in smart combinational therapy.
Dispersion Analysis of Twist-Symmetric Dielectric Waveguides
Authors: Castillo-Tapia Pilar , Van Gassen Kwinten , Chen Qiao , Mesa Francisco , Sipus Zvonimir , Quevedo-Teruel Oscar
Published: June 2021

We propose a circular twist-symmetric dielectric waveguide that is polarization-selective. In the practical implementation of optical fibers, a selective circular polarization is more convenient than its linearly polarized counterpart where previous knowledge of the emitted polarization from the transmitter is unknown. The analysis of the waveguide was conducted with three methods: an eigenmode approach, simulation of a truncated structure, and the so-called multimodal transfer-matrix method (MMTMM). The presented simulations demonstrate that the operational band can be manipulated by tuning the parameters of the structure. Furthermore, the MMTMM allows for a direct and accurate calculation of the attenuation constant of the rejected circular polarization.
Dynamics of H3K27me3 Modification on Plant Adaptation to Environmental Cues
Authors: Shen Qingwen , Lin Yisheng , Li Yingbo , Wang Guifeng
Published: June 2021

Given their sessile nature, plants have evolved sophisticated regulatory networks to confer developmental plasticity for adaptation to fluctuating environments. Epigenetic codes, like tri-methylation of histone H3 on Lys27 (H3K27me3), are evidenced to account for this evolutionary benefit. Polycomb repressive complex 2 (PRC2) and PRC1 implement and maintain the H3K27me3-mediated gene repression in most eukaryotic cells. Plants take advantage of this epigenetic machinery to reprogram gene expression in development and environmental adaption. Recent studies have uncovered a number of new players involved in the establishment, erasure, and regulation of H3K27me3 mark in plants, particularly highlighting new roles in plants’ responses to environmental cues. Here, we review current knowledge on PRC2-H3K27me3 dynamics occurring during plant growth and development, including its writers, erasers, and readers, as well as targeting mechanisms, and summarize the emerging roles of H3K27me3 mark in plant adaptation to environmental stresses.
First Report of Sesame Mutants Tolerant to Severe Drought Stress during Germination and Early Seedling Growth Stages
Authors: Kouighat Mohamed , Hanine Hafida , El Fechtali Mohamed , Nabloussi Abdelghani
Published: June 2021

In the context of climate change and water scarcity, there is a need to develop and use drought-tolerant sesame cultivars. This study was conducted to evaluate the response of 13 sesame genotypes, including 11 mutants and their wild-types, to drought during germination and early seedling growth. Moderate and severe drought stress was simulated by applying polyethylene glycol (PEG) at two osmotic potentials, −0.6 MPa and −1.2 MPa, respectively, on seeds of two successive mutant generations, M2 and M3. The parameters measured or calculated were germination percentage (GP), germination rate (GR), mean germination time (MGT), root length (RL), shoot length (SL), root to shoot ratio (RSR), and the seedling vigor index (SVI). Results showed the significant effect of genotype, drought, and drought × genotype interaction on all parameters investigated. Under severe drought, seeds of seven genotypes, including wild types, were not able to germinate. There was a drastic decline of all parameters for the rest, except MGT and RSR, which markedly increased. Interestingly, two mutants, “ML2-5” and “ML2-10”, were identified as the most tolerant to severe drought and the most stable over both generations. The present work is the first report of sesame germplasm with such a high level of tolerance to drought during germination and early seedling growth stages.
Cultivar Susceptibility to Natural Infections Caused by Fungal Grapevine Trunk Pathogens in La Mancha Designation of Origin (Spain)
Authors: Chacón-Vozmediano Juan L. , Gramaje David , León Maela , Armengol Josep , Moral Juan , Izquierdo-Cañas Pedro M. , Martínez-Gascueña Jesús
Published: June 2021

Grapevine trunk diseases (GTDs) are one of the main biotic stress factors affecting this crop. The use of tolerant grapevine cultivars would be an interesting and sustainable alternative strategy to control GTDs. To date, most studies about cultivar susceptibility have been conducted under controlled conditions, and little information is available about tolerance to natural infections caused by GTD fungi. The objectives of this study were: (i) to identify tolerant cultivars to GTD fungi within a Spanish germplasm collection, based on external symptoms observed in the vineyard; and (ii) to characterize the pathogenic mycoflora associated with symptomatic vines. For this purpose, a grapevine germplasm collection including 22 white and 25 red cultivars was monitored along three growing seasons, and their susceptibility for esca foliar symptoms was assessed. Fungi were identified by using morphological and molecular methods. Cultivars such as, ‘Monastrell’, ‘Graciano’, ‘Cabernet Franc’, ‘Cabernet Sauvignon’, ‘Syrah’, ‘Moscatel de Alejandría’, ‘Sauvignon Blanc’, and ‘Airén’ displayed high susceptibility to GTDs, whereas others such as ‘Petit Verdot’, ‘Pinot Noir’, ‘Chardonnay’, and ‘Riesling’ were considered as tolerant. The prevalent fungal species isolated from symptomatic vines were Phaeomoniella chlamydospora (27.9% of the fungal isolates), Cryptovalsa ampelina (24.6%), and Dothiorella sarmentorum (21.3%).
Plants 2021 Best Paper Award
Authors:   Plants Editorial Office
Published: June 2021

Plants is instituting the Best Paper Awards to recognize the outstanding papers published in the journal [...]
Determination of Fertility-Related Traits in Muscadine Grape Population
Authors: Campbell Jiovan , Gajjar Pranavkumar , Ismail Ahmed , Habibi Fariborz , Darwish Ahmed G. , Tsolova Violeta , Sarkhosh Ali , El-Sharkawy Islam
Published: June 2021

In this study, fertility-related traits of 90 muscadine grape genotypes were evaluated. Selected genotypes included 21 standard cultivars, 60 breeding lines, and nine Vitis × Muscadinia hybrids (VM hybrids). The first fruiting bud (FFB), bud fertility (BF), bud fertility coefficient (BFC), number of flowers/flower cluster (N.F/FC), fruit-set efficiency (FSE), number of clusters/vine (N.C/V), and yield/vine (Y/V) traits were evaluated. The FFB trait did not show significant differences among genotypes. The muscadine genotype O28-4-2-2 (1.6 ± 0.2) displayed the FFB closest to the base; however, O17-16-2-1, O18-2-1, and VM A12-10-2 genotypes had the most distant FFB (3.6 ± 0.3). All the other fertility-related traits varied widely among the population. The BF, BFC, N.F/FC, FSE, N.C/V, and Y/V exhibited a range estimated at 35.1%, 81.5%, 259.7, 63.3%, 177 C/V, and 22.3 kg/V, respectively. The muscadine genotypes O42-3-1 (36.7% ± 1.3) and Majesty (34% ± 1.2) exhibited the highest BF; however, the VM A12-10-2 (1.6% ± 0.1) recorded the lowest BF. The VM genotype O15-16-1 (82.8% ± 4.1) displayed the highest BFC; however, the VM A12-10-2 (1.3% ± 0.1) showed the lowest BFC. The muscadine genotypes D7-1-1 (280.3 F/FC ± 21.7) and O17-17-1 (20.7 F/FC ± 5.5) showed the highest and lowest N.F/FC, respectively. The maximum and minimum FSE was observed for the Rosa cultivar (65.7% ± 2.4) and muscadine genotype D7-1-1 (2.4% ± 0.2), respectively. The minimum N.C/V was recorded for VM genotype A12-10-2 (6 C/V ± 0.2) and maximum noted for muscadine genotypes B20-18-2 (183 C/V ± 7.5) and O44-14-1 (176 C/V ± 7.3). Muscadine genotype O23-11-2 (22.6 kg ± 1.1) produced the highest Y/V; however, the lowest yield was recorded for O15-17-1, Fry Seedless, Sugargate, and the VM genotypes and A12-10-2, with an average yield among them estimated at 0.4 kg ± 0.2.
Evaluating the Endophytic Activities of Beauveria bassiana on the Physiology, Growth, and Antioxidant Activities of Extracts of Lettuce (Lactuca sativa L.)
Authors: Macuphe Neo , Oguntibeju Oluwafemi Omoniyi , Nchu Felix
Published: June 2021

Endophytic entomopathogens have growth promoting, nutrient fortifying, and anti-insect properties that could improve the yield and quality of lettuce (Lactuca sativa L.). Lactuca sativa is a vegetable crop with high demand; however, it is susceptible to aphid infestations. This study’s objectives were to assess the pathogenicity of Beauveria bassiana (strain: SM3) (Bals.) Vuil. (Hypocreales) against Myzus persicae Sulzer, tissue colonization of lettuce by conidia of B. bassiana, as well as the effects of fungal inoculation on growth, tissue nutrient content, and proximate composition of the lettuce plants. Furthermore, the involvement of tissue nutrients in mediating the influence of endophytic fungus on the plant traits was examined. Insects and plants were exposed to four fungal conidial concentrations: 0, 1 × 106, 1 × 107 and 1 × 108 conidia mL−1 in an anti-insect bioassay and a greenhouse experiment, respectively. The B. bassiana strain was pathogenic against M. persicae, inducing mean insect mortality of 78% at the highest concentration (1 × 108 conidia mL−1). The B. bassiana endophytically colonized up to 76% of plants exposed to 1 × 108 conidia mL−1. Crown size and plant height varied significantly among treatments. However, the plant fresh and dry weights and nutrient elements N, P, K, Ca, and Mg did not vary significantly among treatments. Among the plant macronutrients assessed, only tissue carbon content was significantly (p < 0.01) affected by conidial treatments. The tissue C and Cu contents significantly correlated with the antioxidant capacity of the lettuce plants. Most of the micronutrients, viz. Mn, Fe, Cu, and B were remarkably higher (p < 0.05) in the fungus-treated plants than in the control plants. The antioxidant capacity (FRAP and TEAC) of plant extracts varied significantly (p < 0.001) among treatments, with the highest conidial treatment yielding the most increased antioxidant activity. In conclusion, the B. bassiana strain was endophytic to lettuce, pathogenic against M. persicae, and induced increased micro-nutrient tissue contents and antioxidant activities. This study demonstrated that B. bassiana could be potentially used in the biofortification of nutritive and medicinal qualities of plants.
Influence of ER-CR-YSGG Laser and Photodynamic Therapy on the Dentin Bond Integrity of Nano-Hydroxyapatite Containing Resin Dentin Adhesive: SEM-EDX, Micro-Raman, Micro-Tensile, and FTIR Evaluation
Authors: Aljamhan Abdullah S. , Alrefeai Mohammad H. , Alhabdan Alhanouf , Alhusseini Sarah A. , Farooq Imran , Vohra Fahim , Naseem Mustafa , Alkhudhairy Fahad
Published: June 2021

The study aimed to analyze the effect of the addition of nano-hydroxyapatite (nano-HA) particles on the mechanical properties of experimental adhesive (EA). Furthermore, dentin interaction of EA (without nano-HA) and EA with nano-HA (hereon referred to as HA-10%) were also investigated and equated. Methods consisting of scanning electron microscopy (SEM)–energy-dispersive X-ray spectroscopy (EDX), micro-Raman spectroscopy, micro-tensile bond strength (µTBS) test, and Fourier transform infrared (FTIR) spectroscopy were employed to study nano-HA particles shape, dentin bond strength, degree of conversion (DC), and adhesive–dentin interaction. Ninety teeth (N = 90) were collected, and pre-bonding, conditioning of dentin was performed utilizing phosphoric acid (H3PO4) etching, photodynamic therapy (PDT), and ER-CR-YSGG (ECY) laser. The teeth were set to form bonded specimens using two adhesives. Nano-HA particles were spherical-shaped, and EDX confirmed the presence of oxygen, calcium, and phosphorus. Micro-Raman spectroscopy revealed distinct phosphate and carbonate peaks for nano-HA. The µTBS test demonstrated highest values for HA-10% group on the H3PO4 conditioned dentin. The greatest DC was observed for the EA group. The addition of nano-HA-10 wt.% particles in dentin adhesive resulted in improved bond strength. The incorporation also demonstrated acceptable DC (although lower than EA group), suitable dentin interaction, and resin tag formation.
Total Meniscus Reconstruction Using a Polymeric Hybrid-Scaffold: Combined with 3D-Printed Biomimetic Framework and Micro-Particle
Authors: Jeong Hun-Jin , Lee Se-Won , Hong Myoung Wha , Kim Young Yul , Seo Kyoung Duck , Cho Young-Sam , Lee Seung-Jae
Published: June 2021

The meniscus has poor intrinsic regenerative capability, and its injury inevitably leads to articular cartilage degeneration. Although there are commercialized off-the-shelf alternatives to achieve total meniscus regeneration, each has its own shortcomings such as individualized size matching issues and inappropriate mechanical properties. We manufactured a polycaprolactone-based patient-specific designed framework via a Computed Tomography scan images and 3D-printing technique. Then, we completed the hybrid-scaffold by combining the 3D-printed framework and mixture micro-size composite which consists of polycaprolactone and sodium chloride to create a cell-friendly microenvironment. Based on this hybrid-scaffold with an autograft cell source (fibrochondrocyte), we assessed mechanical and histological results using the rabbit total meniscectomy model. At postoperative 12-week, hybrid-scaffold achieved neo-meniscus tissue formation, and its shape was maintained without rupture or break away from the knee joint. Histological and immunohistochemical analysis results showed obvious ingrowth of the fibroblast-like cells and chondrocyte cells as well as mature lacunae that were embedded in the extracellular matrix. Hybrid-scaffolding resulted in superior shape matching as compared to original meniscus tissue. Histological analysis showed evidence of extensive neo-meniscus cell ingrowth. Additionally, the hybrid-scaffold did not induce osteoarthritis on the femoral condyle surface. The 3D-printed hybrid-scaffold may provide a promising approach that can be applied to those who received total meniscal resection, using patient-specific design and autogenous cell source.
Intact Fibrillated 3D-Printed Cellulose Macrofibrils/CaCO3 for Controlled Drug Delivery
Authors: Mohan Denesh , Teong Zee Khai , Sajab Mohd Shaiful , Kamarudin Nur Hidayatul Nazirah , Kaco Hatika
Published: June 2021

The tendency to use cellulose fibrils for direct ink writing (DIW) of three-dimensional (3D) printing has been growing extensively due to their advantageous mechanical properties. However, retaining cellulose in its fibrillated forms after the printing process has always been a challenge. In this study, cellulose macrofibrils (CMFs) from oil palm empty fruit bunch (OPEFB) fibers were partially dissolved for consistent viscosity needed for DIW 3D printing. The printed CMF structure obtained from optimized printing profiles (volumetric flow rate, Qv = 9.58 mm/s; print speed, v = 20 mm/s), exhibited excellent mechanical properties (tensile strength of 66 MPa, Young’s modulus of 2.16 GPa, and elongation of 8.76%). The remarkable structural and morphological effects of the intact cellulose fibrils show a homogeneous distribution with synthesized precipitated calcium carbonate (CaCO3) nanoparticles. The shear-aligned CMF/CaCO3 printed composite exhibited a sustained therapeutic drug release profile that can reduce rapid release that has adverse effects on healthy cells. In comparison with the initial burst release of 5-fluorouracil (5-FU) by CaCO3, the controlled release of 5-fluorouracil can be varied (48 to 75%) with the composition of CMF/CaCO3 allowing efficient release over time.
Special Issue “Metal Nanoparticles as Catalysts for Green Applications”
Authors: Signoretto Michela , Menegazzo Federica
Published: June 2021

This Special Issue of Processes on “Metal Nanoparticles as Catalysts for Green Applications” collects recent works of researchers on metal nanoparticles as catalysts for green applications [...]
Israel and the Individual in Matthew and Midrash: Reassessing “True Israel”
Authors: Schaser Nicholas J.
Published: June 2021

Since the Holocaust, New Testament scholarship has become increasingly sensitive to issues of Christian anti-Judaism. While many Matthean specialists have acknowledged the problems with polemical interpretations of the Gospel, the idea that Matthew presents Jesus and/or the church is the “true Israel” continues to enjoy broad acceptance. The scholarly conflation of Jesus and Israel recycles the Christian polemic against a comparatively inauthentic or inadequate Judaism. This article argues that Matthew does not present Jesus or his church as the true Israel, and that the Jesus-as-Israel interpretation could be refined by comparing the Gospel with later rabbinic discussion that connects Israel with biblical individuals. Genesis Rabbah 40:6 juxtaposes verses about Abraham and Israel to reveal a comprehensive scriptural relationship between the nation and the patriarch without devaluing either party. The rabbis’ theological thesis is predicated on both similarity and separation between Abraham and his offspring. Insofar as both Matthew and Midrash present similar biblical content and exegesis, a comparative analysis can provide Gospel commentators with a view of the Jesus-Israel paradigm that avoids the Christianization of “true Israel.”
Introduction: ‘Religious Transformation in the Middle East: Spirituality, Religious Doubt, and Non-Religion in the Middle East’
Authors: van Nieuwkerk Karin
Published: June 2021

The political impact—or rather the lack thereof—following the revolutionary uprisings in the Middle East has been well documented [...]
Religious Images and Iconoclasm in Reformation Iceland
Authors: Hannesdóttir Sigrún
Published: June 2021

This work assesses what happened to liturgical objects from Icelandic churches and monastic houses during and after the Lutheran Reformation, through an examination of written sources, such as inventories and Visitation books, and material evidence in museum collections and from archaeological excavations. The aim of this work is first, to assess the extent and nature of iconoclasm in Iceland and secondly to re-examine traditional narratives of the Icelandic Reformation in the light of material culture.
God as Highest Truth According to Aquinas
Authors: Martínez Enrique
Published: June 2021

Contemporary public opinion has come to assume that we live in the post-truth era, in which judgments on the most relevant realities of human life have been left in the hands of mere emotions. In such a context, it is very opportune to redirect our gaze toward the concept of truth, in order to help to adequately ground such a primordial reality as that of the personal being. Furthermore, this is the object of the present research, following the thought of St. Thomas Aquinas. To this end, we attempt to argue that the primacy in the analogical significance of the truth corresponds precisely to the person, as a subsistent being whose esse is intelligible to himself. Following the analogical ascent, we consequently arrive at God, who is absolutely intelligible to himself. We have to conclude, therefore, that the personal God is the highest truth. As a corollary to this argument, we add that the perfective dynamism of the personal life is realized in an eminent way in the communication of truth through words, also in God.
CC(U)S Initiatives: Public Effects and “Combined Value” Performance
Authors: Ilinova Alina , Romasheva Natalia , Cherepovitsyn Alexey
Published: June 2021

The changes in climate, which are associated with the emission of anthropogenic greenhouse gases, have been widely discussed by scientists and specialists during the last few decades. The promising way to reduce CO2 emission is to implement CC(U)S technologies (carbon capture, (utilization) and storage). However, CC(U)S initiatives are challenging that prevent their widespread adoption. The main purpose of the research is to prove that CC(U)S should be considered broader than a way to reduce emission, and such initiatives could lead to various public effects and create long-term “combined value” for the industry and wider society; all of these should be considered when making decisions on CC(U)S implementation. The results of the research are presented by highlighting bi-directional interaction between CC(U)S and society, including public acceptance and public effects; identifying the possible positive and negative impact of CC(U)S initiatives on the public; developing a system of indicators for assessing the public effects of CC(U)S; proposing the framework for a value at stake analysis (VAS) of CC(U)S initiatives in order to reveal and assess their “combined value”. The methodology of this study includes desk studies, decomposition technique, environment (E), health (H) and safety (S) (EHS) approach, classification techniques, and VAS analysis.