bluuebunny/crossref_metadata_2025 huggingface dataset:
Chaotic Communication System with Symmetry-Based Modulation
Authors: Karimov Timur , Rybin Vyacheslav , Kolev Georgii , Rodionova Ekaterina , Butusov Denis
Published: April 2021

Communication systems based on chaotic synchronization are gaining interest in the area of secure and covert data transmission. In this paper, a novel digital communication technique based on a coherent chaotic data transmission approach is proposed. In general, this technique resembles the well-known approach based on the modulation of nonlinearity parameters. The key idea of this study is to modulate a signal by varying not the system parameter but the symmetry coefficient in discrete chaotic models obtained by the special numerical integration method. For this purpose, the self-adjoint semi-implicit integration method of order 2 is used to obtain discrete master and slave models of the considered chaotic oscillator. The experimental results explicitly show that, like during parameter modulation, transmitting and receiving oscillators may completely synchronize only if the symmetry coefficients are equal in both systems. The architecture of the communication system based on the proposed modulation is presented. The practical applicability of the approach is confirmed by transmitting a test binary sequence between the transmitter and receiver models and preliminary benchmarking of the obtained communication system. Since the symmetry coefficient modulation does not significantly impact the chaotic behavior of the transmitting digital system, its better suitability for covert messaging was experimentally confirmed by comparing it with the parameter modulation technique.
Characteristics of Warm Mix Asphalt Incorporating Coarse Steel Slag Aggregates
Authors: Alnadish Adham Mohammed , Aman Mohamad Yusri , Katman Herda Yati Binti , Ibrahim Mohd Rasdan
Published: April 2021

The major goal of sustainable practices is to preserve raw resources through the utilization of waste materials as an alternative to natural resources. Decreasing the temperature required to produce asphalt mixes contributes to environmental sustainability by reducing energy consumption and toxic emissions. In this study, warm mix asphalt incorporating coarse steel slag aggregates was investigated. Warm mix asphalt was produced at different temperatures lower than the control asphalt mixes (hot mix asphalt) by 10, 20, and 30 °C. The performances of the control and warm mix asphalt were assessed through laboratory tests examining stiffness modulus, dynamic creep, and moisture sensitivity. Furthermore, a response surface methodology (RSM) was conducted by means of DESIGN EXPERT 11 to develop prediction models for the performance of warm mix asphalt. The findings of this study illustrate that producing warm mix asphalt at a temperature 10 °C lower than that of hot mix asphalt exhibited the best results, compared to the other mixes. Additionally, the warm mix asphalt produced at 30 °C lower than the hot mix asphalt exhibited comparable performance to the hot mix asphalt. However, as the production temperature increases, the performance of the warm mix asphalt improves.
A Technique of Recursive Reliability-Based Missing Data Imputation for Collaborative Filtering
Authors: Ihm Sun-Young , Lee Shin-Eun , Park Young-Ho , Nasridinov Aziz , Kim Miyeon , Park So-Hyun
Published: April 2021

Collaborative filtering (CF) is a recommendation technique that analyzes the behavior of various users and recommends the items preferred by users with similar preferences. However, CF methods suffer from poor recommendation accuracy when the user preference data used in the recommendation process is sparse. Data imputation can alleviate the data sparsity problem by substituting a virtual part of the missing user preferences. In this paper, we propose a k-recursive reliability-based imputation (k-RRI) that first selects data with high reliability and then recursively imputes data with additional selection while gradually lowering the reliability criterion. We also propose a new similarity measure that weights common interests and indifferences between users and items. The proposed method can overcome disregarding the importance of missing data and resolve the problem of poor data imputation of existing methods. The experimental results demonstrate that the proposed approach significantly improves recommendation accuracy compared to those resulting from the state-of-the-art methods while demanding less computational complexity.
Phytochemicals, Antioxidant Attributes and Larvicidal Activity of Mercurialis annua L. (Euphorbiaceae) Leaf Extracts against Tribolium confusum (Du Val) Larvae (Coleoptera; Tenebrionidae)
Authors: Ben Nasr Rania , Baudelaire Elie , Dicko Amadou , El Ferchichi Ouarda Hela
Published: April 2021

This study reports the link between larvicidal activity and the phytochemical composition of male and female leaf extracts of Mercurialis annua L. (M. annua) from four Tunisian regions: Bizerte, Jandouba, Nabeul and Beja. Their antioxidant activity was evaluated using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assays. Phenolic compounds were identified and quantified using liquid chromatography coupled with a UV detector and mass spectrometry (LC-UV-ESI/MS). Higher antioxidant activity (AOA) was found in the leaves of male plant extracts than of female ones. The leaves of male and female plant extracts from Bizerte exhibited the highest AOA: 22.04 and 22.78 mg Trolox equivalent/g dry matter (mg TE/g DM), respectively. For both sexes, plant extracts from Beja had the lowest AOA with 19.71 and 19.67 mg TE/g DM for male and female plants, respectively. Some phenolic compounds such as narcissin, gallocatechin, rutin, epigallocatechin and epicatechin were identified and quantified using LC-MS, which highlighted the abundance of narcissin and rutin in the male leaves of M. annua. We noted that the interaction between the sex of plants and the provenance had a significant effect on TFC (F = 6.63; p = 0.004) and AOA (F = 6.53; p = 0.004) assays, but there was no interaction between sex and origins for TPC (F = 1.76; p = 0.19). The larvicidal activity of aqueous leaf extracts of M. annua against Tribolium confusum (Du Val) (T. confusum), an insect pest of flour and cereal seeds, showed that the mortality could reach 100% after 48 h of exposure in the Bizerte region. The LC50 values for the leaf extract were low in Bizerte, with 0.003 and 0.009 g/mL for male and female plants, respectively, succeeded by Jandouba, which displayed 0.006 and 0.024 g/mL for male and female plants, respectively. Nabeul showed 0.025 g/mL for male plants and 0.046 g/mL for female plants and Beja showed 0.037 and 0.072 g/mL for male and female plants, respectively. This is the first time that a study has revealed a negative correlation between the antioxidant activity and the larvicidal activity of the leaf extracts of M. annua with the following correlation coefficients of Perason: r = −0.975 and r = −0.760 for male and female plants, respectively.
Protein Intrinsic Disorder and Evolvability of MERS-CoV
Authors: Uversky Vladimir N. , Redwan Elrashdy M. , Aljadawi Abdullah A.
Published: April 2021

Middle East Respiratory Syndrome (MERS) is a viral respiratory disease caused by one of the human coronaviruses, MERS-CoV [...]
A Homozygous Dab1−/− Is a Potential Novel Cause of Autosomal Recessive Congenital Anomalies of the Mice Kidney and Urinary Tract
Authors: Racetin Anita , Filipović Natalija , Lozić Mirela , Ogata Masaki , Gudelj Ensor Larissa , Kelam Nela , Kovačević Petra , Watanabe Koichiro , Katsuyama Yu , Saraga-Babić Mirna , Glavina Durdov Merica , Vukojević Katarina
Published: April 2021

This study aimed to explore morphology changes in the kidneys of Dab1−/− (yotari) mice, as well as expression patterns of reelin, NOTCH2, LC3B, and cleaved caspase3 (CASP3) proteins, as potential determinants of normal kidney formation and function. We assumed that Dab1 functional inactivation may cause disorder in a wide spectrum of congenital anomalies of the kidney and urinary tract (CAKUT). Animals were sacrificed at postnatal days P4, P11, and P14. Paraffin-embedded kidney tissues were sectioned and analyzed by immunohistochemistry using specific antibodies. Kidney specimens were examined by bright-field, fluorescence, and electron microscopy. Data were analyzed by two-way ANOVA and t-tests. We noticed that yotari kidneys were smaller in size with a reduced diameter of nephron segments and thinner cortex. TEM microphotographs revealed foot process effacement in the glomeruli (G) of yotari mice, whereas aberrations in the structure of proximal convoluted tubules (PCT) and distal convoluted tubules (DCT) were not observed. A significant increase in reelin expression, NOTCH2, LC3B and cleaved CASP3 proteins was observed in the glomeruli of yotari mice. Renal hypoplasia in conjunction with foot process effacement and elevation in the expression of examined proteins in the glomeruli revealed CAKUT phenotype and loss of functional kidney tissue of yotari.
Gene Therapy for Lysosomal Storage Disorders: Ongoing Studies and Clinical Development
Authors: Massaro Giulia , Geard Amy F. , Liu Wenfei , Coombe-Tennant Oliver , Waddington Simon N. , Baruteau Julien , Gissen Paul , Rahim Ahad A.
Published: April 2021

Rare monogenic disorders such as lysosomal diseases have been at the forefront in the development of novel treatments where therapeutic options are either limited or unavailable. The increasing number of successful pre-clinical and clinical studies in the last decade demonstrates that gene therapy represents a feasible option to address the unmet medical need of these patients. This article provides a comprehensive overview of the current state of the field, reviewing the most used viral gene delivery vectors in the context of lysosomal storage disorders, a selection of relevant pre-clinical studies and ongoing clinical trials within recent years.
Treatment Options for Motor and Non-Motor Symptoms of Parkinson’s Disease
Authors: Church Frank C.
Published: April 2021

Parkinson’s disease (PD) usually presents in older adults and typically has both motor and non-motor dysfunctions. PD is a progressive neurodegenerative disorder resulting from dopaminergic neuronal cell loss in the mid-brain substantia nigra pars compacta region. Outlined here is an integrative medicine and health strategy that highlights five treatment options for people with Parkinson’s (PwP): rehabilitate, therapy, restorative, maintenance, and surgery. Rehabilitating begins following the diagnosis and throughout any additional treatment processes, especially vis-à-vis consulting with physical, occupational, and/or speech pathology therapist(s). Therapy uses daily administration of either the dopamine precursor levodopa (with carbidopa) or a dopamine agonist, compounds that preserve residual dopamine, and other specific motor/non-motor-related compounds. Restorative uses strenuous aerobic exercise programs that can be neuroprotective. Maintenance uses complementary and alternative medicine substances that potentially support and protect the brain microenvironment. Finally, surgery, including deep brain stimulation, is pursued when PwP fail to respond positively to other treatment options. There is currently no cure for PD. In conclusion, the best strategy for treating PD is to hope to slow disorder progression and strive to achieve stability with neuroprotection. The ultimate goal of any management program is to improve the quality-of-life for a person with Parkinson’s disease.
Mineralocorticoid Receptor Antagonists Eplerenone and Spironolactone Modify Adrenal Cortex Morphology and Physiology
Authors: Pereira Sofia , Carvalho Liliana , Costa Madalena , Melo Armindo , Ferreira Isabel , Gomez-Sanchez Celso , Monteiro Mariana , Vinson Gavin , Pignatelli Duarte
Published: April 2021

Mineralocorticoid receptor antagonists (MRAs) are a class of anti-hypertensive drugs that act by blocking aldosterone action. The aim of this study was to evaluate whether the MRAs spironolactone and eplerenone influence adrenal cortical physiology and morphology. Spontaneous hypertensive rats (SHR, n = 18) and normotensive rats (WKY, n = 18) were randomly exposed to a daily dose of spironolactone (n = 6), eplerenone (n = 6), or no drug (n = 6) over 28 days. After that, aldosterone, corticosterone, and 11-deoxycorticosterone plasma concentrations were quantified. Adrenal glands were subjected to morphological analysis to assess lipid droplets content, capsular width, cell proliferation, and steroidogenic proteins expression. The adrenal cortex in untreated SHR showed higher lipid droplet content as than in WKY. In SHR, MRA treatment was associated with higher circulating aldosterone levels and Ki-67 expression in aldosterone-secreting cells. In WKY, the only difference observed after MRA spironolactone treatment was a narrower capsule. There was no difference in abundance of steroidogenic enzyme between groups. In conclusion, MRAs modify adrenal gland function and morphology in SHR. The effects observed within the adrenal glomerulosa with aldosterone-secreting cell proliferation and higher circulating aldosterone levels suggests that MRA treatment provokes activation of the renin angiotensin system. The prognostic value of hyperaldosteronism secondary to MRAs blockade requires further investigation.
Cross-Domain Transfer Learning for PCG Diagnosis Algorithm
Authors: Tseng Kuo-Kun , Wang Chao , Huang Yu-Feng , Chen Guan-Rong , Yung Kai-Leung , Ip Wai-Hung
Published: April 2021

Cardiechema is a way to reflect cardiovascular disease where the doctor uses a stethoscope to help determine the heart condition with a sound map. In this paper, phonocardiogram (PCG) is used as a diagnostic signal, and a deep learning diagnostic framework is proposed. By improving the architecture and modules, a new transfer learning and boosting architecture is mainly employed. In addition, a segmentation method is designed to improve on the existing signal segmentation methods, such as R wave to R wave interval segmentation and fixed segmentation. For the evaluation, the final diagnostic architecture achieved a sustainable performance with a public PCG database.
Facile Synthesis of Flower-Like TiO2-Based Composite for Adsorption–Photocatalytic Degradation of High-Chroma Methylene Blue
Authors: Tian Mengqi , Wang Jingjing , Sun Runjun , Yao Mu , Li Lianbi
Published: April 2021

A flower-like TiO2-based composite (denoted as Zn-Ti-6) was prepared using a flower-like zinc oxide template for adsorption–photocatalytic degradation of high-chroma methylene blue. The reaction took place in an alkaline environment following hydrochloric acid treatment to remove the template and form TiO2-based composite. Sodium hydroxide played both roles of morphology-directing agent and reactive etchant. The possible mechanism for the formation of flower-like Zn-Ti-6 was proposed. The adsorption and photocatalytic degradation behavior of Zn-Ti-6 on methylene blue (MB) removal was also investigated. The results revealed that Zn-Ti-6 showed better adsorption and photocatalytic degradation performance than TiO2 nanoparticles owing to its much larger specific surface area, more abundant hydroxyls, and lower photoluminescence intensity. The adsorption and photocatalytic degradation data of Zn-Ti-6 were well fitted to the pseudo-second-order and pseudo-first-order kinetics models, respectively. The excellent adsorption performance of Zn-Ti-6 is largely beneficial to the subsequent photocatalytic degradation performance for high-chroma wastewater treatment. Overall, this study contributes a facile fabrication strategy for flower-like TiO2-based composite to achieve the adsorption–photocatalytic degradation of high-chroma wastewater.
In Situ IR Studies on the Mechanism of Dimethyl Carbonate Synthesis from Methanol and Carbon Dioxide
Authors: Almusaiteer Khalid A. , Al-Mayman Sulaiman I. , Mamedov Aghaddin , Al-Zeghayer Yousef S.
Published: April 2021

The synthesis of dimethyl carbonate (DMC) from methanol and Carbon dioxide (CO2) has been investigated over 5% Rh/Al2O3 catalyst. Diffuse Reflectance Infrared Fourier Transfer Spectroscopy (DRIFTS) was used to probe the reaction adsorbates which showed that activation of methanol and CO2 involves generation of intermediate methoxy species and formate ingredients, participating in elementary steps of DMC formation. Formation of DMC involves parallel routes comprising interaction of the OH group of Al2O3 through an acid/base mechanism and formate pathway with participation of metal sites. DMC in acid/base pathway is formed via methoxy species to form methoxy carbonate (CH3O)CO2 (active adsorbate), which then reacts with the methyl species to form DMC. The pathway involving metal Rh sites generates an additional elementary step for the involvement of CO2 in the reaction through active formate species. The synergy of parallel pathways determines the performance of the 5% Rh/Al2O3 catalyst. Further improvement of catalyst performance should be based on such a feature of the reaction mechanism.
Lipase Immobilized on MCFs as Biocatalysts for Kinetic and Dynamic Kinetic Resolution of sec-Alcohols
Authors: Stradomska Dominika , Heba Monika , Czernek Aleksandra , Kuźnik Nikodem , Gillner Danuta , Maresz Katarzyna , Pudło Wojciech , Jarzębski Andrzej , Szymańska Katarzyna
Published: April 2021

Dynamic kinetic resolution (DKR) is one of the most attractive methods for enantioselective synthesis. In the reported studies, lipase B from Candida antarctica (CALB) immobilized on siliceous mesoporous cellular foams (MCF) functionalized with different hydrophobic groups, and two ruthenium complexes with substituted cyclopentadienyl ligands were investigated as catalysts for the chemoenzymatic DKR of (rac)-1-phenylethanol, using Novozym 435 as a benchmark biocatalyst. Studies on the (rac)-1-phenylethanol transesterification reaction showed that CALB supported on MCFs grafted with methyl groups is a promising biocatalyst and isopropenyl acetate is a preferable acylation agent. Both Ru-complexes activated by K3PO4 or t-BuOK, proved to be effective catalysts of the racemization reaction. The final DKR experiments using all catalysts combinations singled out, gave 96% conversion, and (R)-1-phenylethyl acetate enantiomeric excess of 98% in 8 h using K3PO4 activator.
Research Progress on Catalytic Water Splitting Based on Polyoxometalate/Semiconductor Composites
Authors: Wu Yue , Bi Lihua
Published: April 2021

In recent years, due to the impact of global warming, environmental pollution, and the energy crisis, international attention and demand for clean energy are increasing. Hydrogen energy is recognized as one of the clean energy sources. Water is considered as the largest potential supplier of hydrogen energy. However, artificial catalytic water splitting for hydrogen and oxygen evolution has not been widely used due to its high energy consumption and high cost during catalytic cracking. Therefore, the exploitation of photocatalysts, electrocatalysts, and photo-electrocatalysts for rapid, cost effective, and reliable water splitting is essentially needed. Polyoxometalates (POMs) are regarded as the potential candidates for water splitting catalysis. In addition to their excellent catalytic properties and reversibly redox activities, POMs can also modify semiconductors to overcome their shortcomings, and improve photoelectric conversion efficiency and photocatalytic activity, which has attracted more and more attention in the field of photoelectric water splitting catalysis. In this review, we summarize the latest applications of POMs and semiconductor composites in the field of photo-electrocatalysis (PEC) for hydrogen and oxygen evolution by catalytic water splitting in recent years and take the latest applications of POMs and semiconductor composites in photocatalysis for water splitting. In the conclusion section, the challenges and strategies of photocatalytic and PEC water-splitting by POMs and semiconductor composites are discussed.
The Role of Galectin-9 as Mediator of Atopic Dermatitis: Effect on Keratinocytes
Authors: Corrêa Mab P. , Areias Libnah L. , Correia-Silva Rebeca D. , D’Ávila Solange C. G. P. , Leopoldino Andréia M. , Greco Karin V. , Gil Cristiane D.
Published: April 2021

Galectin-9 (Gal-9) is a beta-galactoside-binding protein with a variety of biological functions related to immune response. However, in allergic diseases, its mechanism of action is not fully understood. This study evaluates the expression pattern of Gal-9 in patients with atopic dermatitis (AD), in ovalbumin (OVA)-induced experimental atopic dermatitis (AD) in mice, as well as its effect on human keratinocytes. The skin of OVA-immunized BALB/c mice was challenged with drops containing OVA on days 11, 14–18, and 21–24. HaCaT cells were cultured in the following experimental conditions: control (growth medium only) or stimulated with TNF-α/IFN-γ, or IL-4, or IL-17 with or without Gal-9 treatment. AD was characterized by increased levels of Gal-9 in mouse and human skin, especially in the epidermis, and with a marked influx of Gal-9 positive eosinophils and mast cells compared to the control group. Gal-9 showed an immunomodulatory effect on keratinocytes by decreasing the release of IL-6 by IL-4-stimulated keratinocytes or increasing the IL-6 and RANTES levels by IL-17- or TNF-α/IFN-γ-stimulated cells, respectively. Under IL-17, Gal-9 treatment also altered the proliferation rate of cells. Overall, increased levels of Gal-9 in AD skin contribute to the control of inflammatory response and the proliferative process of keratinocytes, suggesting this lectin as a relevant therapeutic target.
Coordinated and Independent Roles for MLH Subunits in DNA Repair
Authors: Pannafino Gianno , Alani Eric
Published: April 2021

The MutL family of DNA mismatch repair proteins (MMR) acts to maintain genomic integrity in somatic and meiotic cells. In baker’s yeast, the MutL homolog (MLH) MMR proteins form three heterodimeric complexes, MLH1-PMS1, MLH1-MLH2, and MLH1-MLH3. The recent discovery of human PMS2 (homolog of baker’s yeast PMS1) and MLH3 acting independently of human MLH1 in the repair of somatic double-strand breaks questions the assumption that MLH1 is an obligate subunit for MLH function. Here we provide a summary of the canonical roles for MLH factors in DNA genomic maintenance and in meiotic crossover. We then present the phenotypes of cells lacking specific MLH subunits, particularly in meiotic recombination, and based on this analysis, propose a model for an independent early role for MLH3 in meiosis to promote the accurate segregation of homologous chromosomes in the meiosis I division.
Targeting Inflammatory Pathways in Cardiovascular Disease: The Inflammasome, Interleukin-1, Interleukin-6 and Beyond
Authors: Libby Peter
Published: April 2021

Recent clinical trials have now firmly established that inflammation participates causally in human atherosclerosis. These observations point the way toward novel treatments that add to established therapies to help stem the growing global epidemic of cardiovascular disease. Fortunately, we now have a number of actionable targets whose clinical exploration will help achieve the goal of optimizing beneficial effects while avoiding undue interference with host defenses or other unwanted actions. This review aims to furnish the foundation for this quest by critical evaluation of the current state of anti-inflammatory interventions within close reach of clinical application, with a primary focus on innate immunity. In particular, this paper highlights the pathway from the inflammasome, through interleukin (IL)-1 to IL-6 supported by a promising body of pre-clinical, clinical, and human genetic data. This paper also considers the use of biomarkers to guide allocation of anti-inflammatory therapies as a step toward realizing the promise of precision medicine. The validation of decades of experimental work and association studies in humans by recent clinical investigations provides a strong impetus for further efforts to target inflammation in atherosclerosis to address the considerable risk that remains despite current therapies.
The Impact of Preprocessing Methods for a Successful Prostate Cell Lines Discrimination Using Partial Least Squares Regression and Discriminant Analysis Based on Fourier Transform Infrared Imaging
Authors: Liberda Danuta , Pięta Ewa , Pogoda Katarzyna , Piergies Natalia , Roman Maciej , Koziol Paulina , Wrobel Tomasz P. , Paluszkiewicz Czeslawa , Kwiatek Wojciech M.
Published: April 2021

Fourier transform infrared spectroscopy (FT-IR) is widely used in the analysis of the chemical composition of biological materials and has the potential to reveal new aspects of the molecular basis of diseases, including different types of cancer. The potential of FT-IR in cancer research lies in its capability of monitoring the biochemical status of cells, which undergo malignant transformation and further examination of spectral features that differentiate normal and cancerous ones using proper mathematical approaches. Such examination can be performed with the use of chemometric tools, such as partial least squares discriminant analysis (PLS-DA) classification and partial least squares regression (PLSR), and proper application of preprocessing methods and their correct sequence is crucial for success. Here, we performed a comparison of several state-of-the-art methods commonly used in infrared biospectroscopy (denoising, baseline correction, and normalization) with the addition of methods not previously used in infrared biospectroscopy classification problems: Mie extinction extended multiplicative signal correction, Eiler’s smoothing, and probabilistic quotient normalization. We compared all of these approaches and their effect on the data structure, classification, and regression capability on experimental FT-IR spectra collected from five different prostate normal and cancerous cell lines. Additionally, we tested the influence of added spectral noise. Overall, we concluded that in the case of the data analyzed here, the biggest impact on data structure and performance of PLS-DA and PLSR was caused by the baseline correction; therefore, much attention should be given, especially to this step of data preprocessing.
MicroRNAs in Medullary Thyroid Carcinoma: A State of the Art Review of the Regulatory Mechanisms and Future Perspectives
Authors: Galuppini Francesca , Censi Simona , Moro Margherita , Carraro Stefano , Sbaraglia Marta , Iacobone Maurizio , Fassan Matteo , Mian Caterina , Pennelli Gianmaria
Published: April 2021

Medullary thyroid carcinoma (MTC) is a rare malignant neoplasia with a variable clinical course, with complete remission often difficult to achieve. Genetic alterations lead to fundamental changes not only in hereditary MTC but also in the sporadic form, with close correlations between mutational status and prognosis. In recent years, microRNAs (miRNAs) have become highly relevant as crucial players in MTC etiology. Current research has focused on their roles in disease carcinogenesis and development, but recent studies have expounded their potential as biomarkers and response predictors to novel biological drugs for advanced MTC. One such element which requires greater investigation is their mechanism of action and the molecular pathways involved in the regulation of gene expression. A more thorough understanding of these mechanisms will help realize the promising potential of miRNAs for MTC therapy and management.
Salicylic Acid Accumulation Controlled by LSD1 Is Essential in Triggering Cell Death in Response to Abiotic Stress
Authors: Bernacki Maciej Jerzy , Rusaczonek Anna , Czarnocka Weronika , Karpiński Stanisław
Published: April 2021

Salicylic acid (SA) is well known hormonal molecule involved in cell death regulation. In response to a broad range of environmental factors (e.g., high light, UV, pathogens attack), plants accumulate SA, which participates in cell death induction and spread in some foliar cells. LESION SIMULATING DISEASE 1 (LSD1) is one of the best-known cell death regulators in Arabidopsis thaliana. The lsd1 mutant, lacking functional LSD1 protein, accumulates SA and is conditionally susceptible to many biotic and abiotic stresses. In order to get more insight into the role of LSD1-dependent regulation of SA accumulation during cell death, we crossed the lsd1 with the sid2 mutant, caring mutation in ISOCHORISMATE SYNTHASE 1(ICS1) gene and having deregulated SA synthesis, and with plants expressing the bacterial nahG gene and thus decomposing SA to catechol. In response to UV A+B irradiation, the lsd1 mutant exhibited clear cell death phenotype, which was reversed in lsd1/sid2 and lsd1/NahG plants. The expression of PR-genes and the H2O2 content in UV-treated lsd1 were significantly higher when compared with the wild type. In contrast, lsd1/sid2 and lsd1/NahG plants demonstrated comparability with the wild-type level of PR-genes expression and H2O2. Our results demonstrate that SA accumulation is crucial for triggering cell death in lsd1, while the reduction of excessive SA accumulation may lead to a greater tolerance toward abiotic stress.
4D BIM for Construction Logistics Management
Authors: Whitlock Kane , Abanda Fonbeyin Henry , Manjia Marcelline Blanche , Pettang Chrispin , Nkeng George Elambo
Published: April 2021

This study presents an investigation into the extent to which emerging building information modelling (BIM) can be applied to construction logistics management (CLM). Given the specialist nature of the domains, the study employed an in-depth qualitative interview, whereby six experts were interrogated about their experiences of BIM for CLM. The study found the main applications of BIM on CLM to be the creation of three-dimensional (3D) site layout plans and four-dimensional (4D) coordination of site processes and common user plant, updating the 4D logistics plan as the project progressed and collaboration in BIM-based logistics coordination. Furthermore, there was a consensus amongst interviewees on improvement in site safety, comprehension of logistics information, efficiency on site, and effectiveness of layout planning as the main benefits. Lastly, the lack of training with implications on understanding was one of the main barriers to applying BIM to CLM. The findings from this study have the potential to stimulate the uptake of BIM by construction logistics practitioners. By so doing, the performance construction project delivery can be improved, and waste can be minimised or eliminated.
Hybrid PV System with High Speed Flywheel Energy Storage for Remote Residential Loads
Authors: Soomro Abid , Pullen Keith R. , Amiryar Mustafa E.
Published: April 2021

Due to low system inertia in microgrids, frequencies may vary rapidly from the nominal value, leading to the complete blackout of the system unless there is an adequate spinning reserve available for balancing the supply with the demand load. This issue of instability in microgrids under islanded operation has attracted particular attention recently. A diesel generator is considered to be an ideal spinning reserve to provide back-up power to the load along with the renewable energy source in islanded system. However, the high maintenance cost and CO2 emissions of diesel generator are detrimental factors which have inspired searches for more cost effective and cleaner technologies. The integration of an energy storage system (ESS) in islanded system along with generator not only reduces generator maintenance costs but also reduces the CO2 emissions by limiting its operating hours. This paper proposes an islanded PV hybrid microgrid system (PVHMS) utilizing flywheel energy storage systems (FESS) as an alternative to battery technology to support the PV system and meet the peak demand of a small residential town with 100 dwellings. The diesel generator is used in the islanded system as a spinning reserve to maintain the stability of the islanded system when the PV system and flywheel storage cannot meet the load demand. Results of analysis of such a system demonstrate that flywheel energy storage technology of appropriate size offers a viable solution to support the operation of the standalone PV system. Furthermore, the reduction in CO2 emissions and fuel consumption has been quantified as compared with the case with flywheel energy storage systems which means the diesel generator but always be operating.
Microstructure and Interfaces of Ultra-Thin Epitaxial AlN Films Grown by Plasma-Enhanced Atomic Layer Deposition at Relatively Low Temperatures
Authors: Goswami Ramasis , Qadri Syed , Nepal Neeraj , Eddy Charles
Published: April 2021

We demonstrate the growth of ultra-thin AlN films on Si (111) and on a GaN/sapphire (0001) substrate using atomic layer epitaxy in the temperature range of 360 to 420 °C. Transmission electron microscopy and X-ray diffraction were used to characterize the interfaces, fine scale microstructure, and the crystalline quality of thin films. Films were deposited epitaxily on Si (111) with a hexagonal structure, while on the GaN/sapphire (0001) substrate, the AlN film is epitaxial and has been deposited in a metastable zinc-blende cubic phase. Transmission electron microscopy reveals that the interface is not sharp, containing an intermixing layer with cubic AlN. We show that the substrate, particularly the strain, plays a major role in dictating the crystal structure of AlN. The strain, estimated in the observed orientation relation, is significantly lower for cubic AlN on hexagonal GaN as compared to the hexagonal AlN on hexagonal GaN. On the Si (111) substrate, on the other hand, the strain in the observed orientation relation is 0.8% for hexagonal AlN, which is substantially lower than the strain estimated for the cubic AlN on Si(111).
Displacements in the Cationic Motif of Nonstoichiometric Fluorite Phases Ba1−xRxF2+x as a Result of the Formation of {Ba8[R6F68–69]} Clusters: III. Defect Cluster Structure of the Nonstoichiometric Phase Ba0.69La0.31F2.31 and Its Dependence on Heat Treatment
Authors: Sulyanova Elena A. , Karimov Denis N. , Sobolev Boris P.
Published: April 2021

The defect structure of Ba0.69La0.31F2.31 single crystals in as-grown state and after annealing at 1173 K for 336 h was studied by X-ray diffraction analysis. Both crystals belong to the CaF2 structure type (sp. gr. Fm3¯m). They have vacancies in the main anion motif and interstitial fluorine anions in Wyckoff positions 48i and 4b. Relaxation (static displacement of some main anions to Wyckoff position 32f) is observed in the annealed crystal. It was established that annealing leads to a change in the type of displacement of the main anions in Wyckoff positions 8c from dynamic to static. Displacement of La3+ cations to Wyckoff position 32f is observed in both crystals. A model of the defect structure of Ba0.69La0.31F2.31 is proposed, according to which interstitial fluorine anions and La3+ cations are aggregated into [Ba14−nLanF64+n] clusters with the cuboctahedral anionic core formed by interstitial fluorine anions in Wyckoff positions 48i. Ba2+ cations are located in the cluster in the centers of the faces, and the La3+ cations are shifted by 0.24 Å from the vertices of the cluster along the three-fold axis towards the center of the cluster. The study establishes the relationship between the defect structure of crystals and their structurally sensitive properties, and to develop approaches to their management.
Effect of Fluoroalkyl-Substituent in Bistolane-Based Photoluminescent Liquid Crystals on Their Physical Behavior
Authors: Yamada Shigeyuki , Wang Yizhou , Morita Masato , Zhang Qingzhi , O’Hagan David , Nagata Masakazu , Agou Tomohiro , Fukumoto Hiroki , Kubota Toshio , Hara Mitsuo , Konno Tsutomu
Published: April 2021

Photoluminescent liquid crystals (PLLCs) have attracted significant attention owing to their broad applicability in thermosensing and PL switching. Extensive efforts have been made to develop bistolane-based PLLCs containing flexible units at both molecular terminals, and it has been revealed that their PL behavior can switch with the phase transition between the crystalline and LC phases. Although slight modulation of the flexible unit structure dramatically alters the LC and PL behaviors, few studies into the modification of the flexible units have been conducted. With the aim of achieving dynamic changes in their physical behaviors, we developed a family of bistolane derivatives containing a simple alkyl or a fluoroalkyl flexible chain and carried out a detailed systematic evaluation of their physical behaviors. Bistolanes containing a simple alkyl chain showed a nematic LC phase, whereas switching the flexible chain in the bistolane to a fluoroalkyl moiety significantly altered the LC phase to generate a smectic phase. The fluoroalkyl-containing bistolanes displayed a stronger deep blue PL than their corresponding non-fluorinated counterparts, even in the crystalline phase, which was attributed to the construction of rigid molecular aggregates through intermolecular F···H and F···F interactions to suppress non-radiative deactivation.
SeeCucumbers: Using Deep Learning and Drone Imagery to Detect Sea Cucumbers on Coral Reef Flats
Authors: Li Joan Y. Q. , Duce Stephanie , Joyce Karen E. , Xiang Wei
Published: April 2021

Sea cucumbers (Holothuroidea or holothurians) are a valuable fishery and are also crucial nutrient recyclers, bioturbation agents, and hosts for many biotic associates. Their ecological impacts could be substantial given their high abundance in some reef locations and thus monitoring their populations and spatial distribution is of research interest. Traditional in situ surveys are laborious and only cover small areas but drones offer an opportunity to scale observations more broadly, especially if the holothurians can be automatically detected in drone imagery using deep learning algorithms. We adapted the object detection algorithm YOLOv3 to detect holothurians from drone imagery at Hideaway Bay, Queensland, Australia. We successfully detected 11,462 of 12,956 individuals over 2.7ha with an average density of 0.5 individual/m2. We tested a range of hyperparameters to determine the optimal detector performance and achieved 0.855 mAP, 0.82 precision, 0.83 recall, and 0.82 F1 score. We found as few as ten labelled drone images was sufficient to train an acceptable detection model (0.799 mAP). Our results illustrate the potential of using small, affordable drones with direct implementation of open-source object detection models to survey holothurians and other shallow water sessile species.
Taxation of Land and Economic Growth
Authors: Che Shulu , Kumar Ronald Ravinesh , Stauvermann Peter J.
Published: April 2021

In this paper, we theoretically analyze the effects of three types of land taxes on economic growth using an overlapping generation model in which land can be used for production or consumption (housing) purposes. Based on the analyses in which land is used as a factor of production, we can confirm that the taxation of land will lead to an increase in the growth rate of the economy. Particularly, we show that the introduction of a tax on land rents, a tax on the value of land or a stamp duty will cause the net price of land to decline. Further, we show that the nationalization of land and the redistribution of the land rents to the young generation will maximize the growth rate of the economy.
The Effects of Corruption, Renewable Energy, Trade and CO2 Emissions
Authors: Leitão Nuno Carlos
Published: April 2021

Corruption reflects a set of illegal activities that jeopardize the smooth functioning of economies, society, and climate and environmental issues. This article tests the relationships between economic growth, corruption, renewable energies, international trade, and carbon dioxide emissions using panel data for European countries, namely Portugal, Spain, Italy, Ireland, and Greece, from 1995–2015. As an econometric strategy, this research uses the panel fully modified least squares (FMOLS), panel dynamic least squares (DOLS), and panel two-stage least squares estimator (TSLS). Considering the variables utilized in the research and the panel unit root test, we observed that the variables are integrated I (1) in the first difference. The variables of corruption, economic growth, renewable energies, international trade, and carbon dioxide emissions are cointegrated in the long run, using the Pedroni and Kao residual cointegration test arguments. The methodology of Dumitrescu–Hurlin to test the causality between carbon dioxide emissions, corruption, economic growth, and renewable energy shows that there is unidirectional causality between carbon dioxide emissions and corruption and economic growth and corruption. The results suggest that the corruption index and economic growth have a statistically significant positive impact on carbon dioxide emissions. However, renewable energies and international trade reduce climate change and improve the environmental quality.
A Critical Review of the Equivalent Stoichiometric Cloud Model Q9 in Gas Explosion Modelling
Authors: Tam Vincent , Tan Felicia , Savvides Chris
Published: April 2021

Q9 is widely used in industries handling flammable fluids and is central to explosion risk assessment (ERA). Q9 transforms complex flammable clouds from pressurised releases to simple cuboids with uniform stoichiometric concentration, drastically reducing the time and resources needed by ERAs. Q9 is commonly believed in the industry to be conservative but two studies on Q9 gave conflicting conclusions. This efficacy issue is important as impacts of Q9 have real life consequences, such as inadequate engineering design and risk management, risk underestimation, etc. This paper reviews published data and described additional assessment on Q9 using the large-scale experimental dataset from Blast and Fire for Topside Structure joint industry (BFTSS) Phase 3B project which was designed to address this type of scenario. The results in this paper showed that Q9 systematically underpredicts this dataset. Following recognised model evaluation protocol would have avoided confusion and misinterpretation in previous studies. It is recommended that the modelling concept of Equivalent Stoichiometric Cloud behind Q9 should be put on a sound scientific footing. Meanwhile, Q9 should be used with caution; users should take full account of its bias and variance.
Raffaelea quercina sp. nov. Associated with Cork Oak (Quercus suber L.) Decline in Portugal
Authors: Inácio Maria L. , Marcelino José , Lima Arlindo , Sousa Edmundo , Nóbrega Filomena
Published: April 2021

Research Highlights: Raffaelea quercina sp. nov. is an ophiostomatoid fungus isolated from the ambrosia beetle Platypus cylindrus. The species occurs in symptomatic Portuguese cork oak trees, (Quercus suber L.), exhibiting vegetative decline. Background and Objectives: Quercus suber L. is a species restricted to the Mediterranean basin, of special economic importance as it constitutes the crucial raw material for the cork production industry, in particular for Portugal, the world’s leading producer. Over the last three decades a progressive and alarming decline of cork oak trees has been observed across its distribution area, including Portugal. The ambrosia beetle Platypus cylindrus, commonly known as the oak pinhole borer, establishes symbiotic relationships with fungi from which it depends for survival and for oak colonization. Some of these fungi are ophiostomatoid species of the Raffaelea genus, known as ambrosia fungi associated with ambrosia beetles. Some Raffaelea species exhibit phytopathogenic activity causing wilting and/or death of trees. The objective of the present study is to identify the association between P. cylindrus and Raffaelea species in Portuguese cork oak stands showing symptoms of disease and decline. Materials and Methods: A total of 300 adult insects were collected as they emerged from cork oak logs, sampled from symptomatic trees. Axenic isolates of Raffaelea species were obtained from the beetles and their galleries in the trunks and identified based on morphological features and molecular analysis of the SSU and LSU rDNA regions. Results: Two Raffaelea species were identified, i.e., R. montetyi and a novel Raffaelea species closely related to R. canadensis. The novel species is morphologically and genetically characterized in this study, and erected as Raffaelea quercina M.L. Inácio, E. Sousa & F. Nóbrega, sp. nov. Raffaelea quercina constitutes a new phytopathogenic fungal species associated with P. cylindrus and cork oak trees exhibiting symptoms of vegetative decline. Conclusions: Raffaelea species appear to have a significant role in cork oak decline. Future research on the association between P. cylindrus and Raffaelea species, encompassing the trans-European and North African wide-range of cork oak stands, would further clarify the relationships between ambrosia beetles, associated fungi and cork oak decline, contributing to a better understanding of the phenomena and for strategies aiming to halt the continuous decline of the unique cork oak stands enclosed in the Mediterranean basin.
A Computational Analysis of the Influence of a Pressure Wire in Evaluating Coronary Stenosis
Authors: Yi Jie , Tian Fang-Bao , Simmons Anne , Barber Tracie
Published: April 2021

Cardiovascular disease is one of the world’s leading causes of morbidity and mortality. Fractional flow reserve (FFR) was proposed in the 1990s to more accurately evaluate the functional severity of intermediate coronary stenosis, and it is currently the gold standard in cardiac catheterization laboratories where coronary pressure and flow are routinely obtained. The clinical measurement of FFR relies on a pressure wire for the recording of pressures; however, in computational fluid dynamics studies, an FFR is frequently predicted using a wire-absent model. We aim to investigate the influence of the physical presence of a 0.014-inch (≈0.36 mm) pressure wire in the calculation of virtual FFR. Ideal and patient-specific models were simulated with the absence and presence of a pressure wire. The computed FFR reduced from 0.96 to 0.93 after inserting a wire in a 3-mm non-stenosed (pipe) ideal model. In mild stenotic cases, the difference in FFR between the wire-absent and wire-included models was slight. The overestimation in severe case was large but is of less clinical significance because, in practice, this tight lesion does not require sophisticated measurement to be considered critical. However, an absence of the pressure wire in simulations could contribute to an over-evaluation for an intermediate coronary stenosis.
Phytochemicals in Food and Health
Authors: Rai Dilip K.
Published: April 2021

Consumption of plant-based diets, rich in phytochemicals, has been associated with reduced risk of degenerative diseases, improved overall health and well-being [...]
Some New Results on F-Contractions in 0-Complete Partial Metric Spaces and 0-Complete Metric-Like Spaces
Authors: Radenović Stojan , Mirkov Nikola , Paunović Ljiljana R.
Published: April 2021

Within this manuscript we generalize the two recently obtained results of O. Popescu and G. Stan, regarding the F-contractions in complete, ordinary metric space to 0-complete partial metric space and 0-complete metric-like space. As Popescu and Stan we use less conditions than D. Wardovski did in his paper from 2012, and we introduce, with the help of one of our lemmas, a new method of proving the results in fixed point theory. Requiring that the function F only be strictly increasing, we obtain for consequence new families of contractive conditions that cannot be found in the existing literature. Note that our results generalize and complement many well-known results in the fixed point theory. Also, at the end of the paper, we have stated an application of our theoretical results for solving fractional differential equations.