bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Leaves and Seeds of Cucurbitaceae Plants as a New Source of Bioactive Compounds for Colorectal Cancer Prevention and Treatment
Authors: Peña Mercedes , Guzmán Ana , Mesas Cristina , Porres Jesús M. , Martínez Rosario , Bermúdez Francisco , Melguizo Consolación , Cabeza Laura , Prados Jose
Published: December 2024

Background/Objectives: The Cucurbitaceae family represents an important source of bioactive compounds with antioxidant, antimicrobial, anti-inflammatory and antitumor activities. This study aims to investigate the potential application of Cucurbitaceae leaves and seed extracts to prevent and treat colorectal cancer (CRC). Methods: Four extracts (ethanol extracts and protein extracts and hydrolysates) from the leaves and seeds of cucurbits were tested in T-84, HCT-15 and HT-29 CRC cells. The antitumor, antiangiogenic, antioxidant and chemopreventive potentials and bioactive composition of the active extracts were characterized. Results: Cold ethanolic extracts from the leaves and seeds of two interspecific Cucurbita genera (CLU01002 and COK01001) exhibited potent antiproliferative, specific and non-hepatotoxic activity against CRC cell lines, with a slight synergistic effect in combination with oxaliplatin. This antitumor activity was related to G2/M cell cycle arrest, the extrinsic apoptosis pathway, cytokinesis inhibition and autophagy. The extracts also inhibited tumor clonogenicity and angiogenesis, and modulated cancer stem cell (CSC) gene expression, as well as expressing antioxidant and chemopreventive cellular capabilities. Finally, phenolic and cucurbitane-type triterpenoid compounds (pengxianencins and cucurbitacins) were tentatively identified in the active extracts by UPLC-MS analysis and bioguided fractionation. Conclusions: Extracts from leaves the and seeds of two interspecific Cucurbita genera (CLU01002 and COK01001) may contribute to the improvement of prevention and treatment strategies for CRC patients.
Growth, Filtration and Respiration Under Superfluous Feeding in Single-Osculum Halichondria panicea Sponges
Authors: Kumala Lars , Riisgård Hans Ulrik
Published: December 2024

Filter-feeding sponges capture suspended food particles from ambient water, but little is known about the response of sponges to high food concentrations causing superfluous feeding. Here, through several experimental assays, we studied the relationship between algal concentration in the water column, filtration rate, respiration rate, and specific growth rate in single-osculum Halichondria panicea demosponge explants. Laboratory experiments showed that sponge explants filter the ambient seawater at a maximum rate when exposed to naturally (low) algal concentrations, whereas high algal concentrations resulted in superfluous feeding and reduced filtration rates. Explants maintained at algal concentrations above the incipient overloading concentration level showed that growth rates were in fair agreement with the maximum possible weight-specific growth rate of about 4% d−1. Although the filtration rate became reduced due to overloading, the oxygen extraction efficiency increased, and therefore superfluous feeding did not cause reduced growth. This suggests that H. panicea and probably other sponges have adapted to low algal concentrations, displaying continuous maximum filtration rates. Osculum closure reflects a protection mechanism rather than a physiological regulatory response to high algal concentrations.
Diastereomeric N,S-Dialkyl Dithiocarbamates Derived from (E)-Chalcones and ʟ-Tryptophan: Microwave-Assisted Synthesis and In Vitro Studies Against Fusarium oxysporum
Authors: Agudelo-Ibañez Natalia , Torres-Cortés Sergio , Coy-Barrera Ericsson , Buitrago Ivon , Quiroga Diego
Published: December 2024

The synthesis of indole phytoalexin-like analogs related to alkyl (((1-(4-substitutedphenyl)-3-oxo-3-phenylpropyl)thio)carbonothioyl)-ʟ-tryptophanate 1a–d and the evaluation of their antifungal activity against the phytopathogen Fusarium oxysporum is reported. The target compounds were synthesized in the following two stages: (1) the initial esterification of ʟ-tryptophan, which reacted with trimethyl silane chloride and simple aliphatic alcohols (R = Me, Et) under microwave irradiation (MWI) at 100 °C to obtain the respective alkyl ester 2a–b; (2) the resulting mixture of ʟ-tryptophanates 2a–b with carbon disulfide and (E)-chalcone 3a–b under MWI at 50 °C during 60 min, followed by purification through classical column chromatography (55–76% yields). The products were obtained as mixtures of (S,R) and (S,S) diastereoisomers. An LC-DAD-MS analysis allowed us to establish the ratio of these diastereoisomers, and subsequent DFT/B3LYP-based computational calculations of the NMR 1H chemical shifts suggested that the major diastereoisomer involved an (S,R) absolute configuration, comprising more than 60% of the mixture. The compounds 1a–d were subjected to an antifungal activity test against the phytopathogen F. oxysporum using an amended medium-based assay. Compound series 1 showed inhibition percentages of 80% at the first concentration and IC50 values between 0.33 and 5.71 mM, demonstrating greater potential as antifungal agents compared to other ʟ-tryptophan derivatives like alkyl (2S)-3-(1H-indol-3-yl)-2-{[(1Z)-3-oxobut-1-en-1-yl]amino}propanoate, which presented lower inhibition percentages. In summary, phytoalexin analogs derived from ʟ-tryptophan and (E)-chalcones significantly inhibited the mycelial growth of Fusarium oxysporum, indicating their potential as effective antifungal agents.
In Vitro Antibacterial Activity of Essential Oils from Origanum vulgare, Satureja montana, Thymus vulgaris, and Their Blend Against Necrotoxigenic (NTEC), Enteropathogenic (EPEC), and Shiga-Toxin Producing Escherichia coli (STEC) Isolates
Authors: Cagnoli Giulia , Bertelloni Fabrizio , Ebani Valentina Virginia
Published: December 2024

Enteropathogenic (EPEC), necrotoxigenic (NTEC), and Shiga-toxin producing Escherichia coli (STEC) are pathotypes responsible for severe clinical forms in humans and animals. They can be shed in the feces of animals with consequent environmental contamination. This study evaluated the antibacterial activity of essential oils (EOs) from oregano (Origanum vulgare), savory (Satureja montana), thyme (Thymus vulgaris), and their blend against EPEC, NTEC, and STEC strains previously isolated from avian fecal samples. Minimum inhibitory concentration values between 0.039% and 0.156% were found with O. vulgare EO, between ≤0.0195% and 0.156% with both S. montana and T. vulgaris EOs, and between 0.039% and ≤0.0195% with the blend. The mixture with equal parts of EOs from oregano, savory and thyme seems a promising alternative product to combat pathogenic E. coli strains responsible for environmental contamination.
Effect of Lactic Acid Bacteria-Derived Postbiotic Supplementation on Tuberculosis in Wild Boar Populations
Authors: Bravo Maria , Gonçalves Pilar , García-Jiménez Waldo , Montero María José , Cerrato Rosario , Fernández-Llario Pedro , Risco David
Published: December 2024

The Eurasian wild boar (Sus scrofa) is a key wildlife host for tuberculosis (TB) in central and southwestern Spain, posing a challenge to TB eradication in livestock. New strategies, including the use of beneficial microbes, are being explored to mitigate wildlife diseases. This study evaluated the effect of oral supplementation with postbiotic antimycobacterial metabolites produced using Ingulados’ lactic acid bacteria (LAB) collection on TB development in wild boar. A total of 20 game estates in mid-western Spain were divided into two groups: one fed with standard feed containing the postbiotic product and a control group fed without postbiotics. Data were collected from wild boar during hunting events pre- and post-supplementation. The presence of TB-like lesions (TBLLs), lesion severity and seropositivity against Mycobacterium bovis were assessed. Postbiotic supplementation led to a 36.87% reduction in TBLLs and a 35.94% decrease in seropositivity. Notably, young wild boar showed a 64.72% reduction in TBLLs and an 81.80% drop in seropositivity, suggesting reduced transmission. These findings support the potential of postbiotics as a safe, feasible and sustainable tool to control TB in wild boar, offering a promising addition to broader TB eradication efforts.
Seasonal Dynamics of Ticks and Tick-Borne Pathogens in Republic of Korea
Authors: Monoldorova Sezim , Lee Sungkyeong , Yun Seungri , Park Sunho , Jeong Jong-Uk , Kim Jiro , Lee In-Yong , Jun Hojong , Park Chan-Ho , Byeon Hyeon-Seop , Han Mina , Youn So-Youn , Cho Yun-Sang , Yun Young-Min , Lee Kwang-Jun , Jeon Bo-Young
Published: December 2024

Tick-borne diseases are a public health problem and a significant burden on the livestock industry. The seasonal abundance of ticks and tick-borne pathogens strongly correlates with the prevalence of these diseases. To investigate the seasonal variation in ticks and tick-borne pathogens, ticks were collected from Gangwon State, Korea, and the tick-borne pathogens Borrelia, Anaplasma, Babesia, and Theileria were examined. In total, 14,748 ticks were collected, comprising ticks from two genera and three species: Haemaphysalis longicornis, Haemaphysalis flava, and Ixodes nipponensis, with H. longicornis being the predominant species. Of 7445 ticks (455 pools) examined for pathogens, Theileria was detected in 61 pools, whereas Borrelia and Anaplasma were observed in 17 pools. H. longicornis nymphs and adults were collected beginning in April, with nymph numbers peaking in May and June and adult ticks peaking in June and July. In contrast, the larvae were collected in May and peaked in September. Tick-borne pathogens were detected in April, peaking in July and September. Borrelia, the causative agent of Lyme disease, exhibits a temporal association between its detection in ticks and its occurrence in humans. In conclusion, tick-borne diseases seem to be closely linked not only to changes in tick numbers throughout the seasons but also to the seasonal variations of the pathogens within them.
H3K4 Methylation and Demethylation in Fungal Pathogens: The Epigenetic Toolbox for Survival and Adaptation in the Host
Authors: Rai Maruti Nandan , Rai Rikky
Published: December 2024

Pathogenic fungi represent a diverse group of eukaryotic microorganisms that significantly impact human health and agriculture. In recent years, the role of epigenetic modifications, particularly histone modifications, in fungal pathobiology has emerged as a prominent area of interest. Among these modifications, methylation of histone H3 at lysine-4 (H3K4) has garnered considerable attention for its implications in regulating gene expression associated with diverse cellular processes. A body of literature has uncovered the pivotal roles of H3K4 methylation in multiple biological processes crucial for pathogenic adaptation in a wide range of fungal pathogens of humans and food crops. This review delves into the recent advancements in understanding the impact of H3K4 methylation/demethylation on fungal pathogenesis. We explore the roles of H3K4 methylation in various cellular processes, including fungal morphogenesis and development, genome stability and DNA repair, metabolic adaptation, cell wall maintenance, biofilm formation, antifungal drug resistance, and virulence. We also discuss the conservation of H3K4 methylation regulators and their potential as therapeutic targets to prevent fungal diseases. Collectively, this review underscores the intricate links between H3K4 methylation, fungal pathogenesis, and potential avenues for novel antifungal strategies.
An Evaluation of the Cellular and Humoral Response of a Multi-Epitope Vaccine Candidate Against COVID-19 with Different Alum Adjuvants
Authors: Vega Rojas Lineth Juliana , Ruíz-Manzano Rocío Alejandra , Velasco-Elizondo Miguel Andrés , Carbajo-Mata María Antonieta , Hernández-Silva Diego Josimar , Rocha-Solache Mariana , Hernández Jesús , Pérez-Serrano Rosa Martha , Zaldívar-Lelo de Larrea Guadalupe , García-Gasca Teresa , Mosqueda Juan
Published: December 2024

SARS-CoV-2 (Betacoronavirus pandemicum) is responsible for the disease identified by the World Health Organization (WHO) as COVID-19. We designed “CHIVAX 2.1”, a multi-epitope vaccine, containing ten immunogenic peptides with conserved B-cell and T-cell epitopes in the receceptor binding domain (RBD) sequences of different SARS-CoV-2 variants of concern (VoCs). We evaluated the immune response of mice immunized with 20 or 60 µg of the chimeric protein with two different alum adjuvants (Alhydrogel® and Adju-Phos®), plus PHAD®, in a two-immunization regimen (0 and 21 days). Serum samples were collected on days 0, 21, 31, and 72 post first immunization, with antibody titers determined by indirect ELISA, while lymphoproliferation assays and cytokine production were evaluated by flow cytometry. The presence of neutralizing antibodies was assessed by surrogate neutralization assays. Higher titers of total IgG, IgG1, and IgG2a antibodies, as well as increased proliferation rates of specific CD4+ and CD8+ T cells, were observed in mice immunized with 60 μg of protein plus Adju-Phos®/PHAD®. This formulation also generated the highest levels of TNF-α and IFN-γ, in addition to the presence of neutralizing antibodies against Delta and Omicron VoC. These findings indicate the potential of this chimeric multi-epitope vaccine with combined adjuvants as a promising platform against viral infections, eliciting a TH1 or TH1:TH2 balanced cell response.
The Comparative Effect of Morphine on Proliferation of Cancer Cell Lines Originating from Different Organs: An In Vitro Study
Authors: Whitham Lydia , Sheikh Mahdi , Hollmann Markus W. , Parat Marie-Odile
Published: December 2024

Background/Objectives: Opium consumption was recently classified by the International Agency for Research on Cancer (IARC) monograph as carcinogenic to humans based on strong evidence for cancers of the larynx, lung, and urinary bladder, and limited evidence for cancers of the oesophagus, stomach, pancreas, and pharynx. This poses the question of a potential pro-cancer effect of pharmaceutical opioid analgesics. In vitro studies employing a variety of experimental conditions suggest that opioid alkaloids have proliferative or antiproliferative effects. We set out to reconcile this discrepancy and explore the hypothesis that opioids promote cancer cell proliferation in an organ-dependent fashion. Methods: Using strictly controlled conditions, we tested the effect of morphine on the proliferation of a series of human cancer cell lines isolated from organs where cancer risk was linked causally to opium consumption in human studies (i.e., lung, bladder, and larynx), or control organs where no link between cancer risk and opium consumption has been reported in human studies (i.e., breast, colon, prostate). Results: Our results showed a minimal effect on proliferation on any cell line and no trend supporting an organ-specific effect of morphine. Conclusions: This argues against a direct effect of opioids on tumour cell proliferation to support their organ-specific effect.
An Effective Method for Generating Isolated Attosecond Pulses from a Solid Crystal Film
Authors: Lv Wenwen , Wang Feng , Gao Congzhang
Published: December 2024

Solids subjected to strong-field laser excitation can produce high harmonics, making high-order harmonic generation (HHG) one of the most effective methods for creating ultrafast coherent light sources, such as isolated attosecond pulses (IAPs). While extensive research has been conducted on generating IAPs through HHG in gaseous media, studies focusing on solid media are relatively limited. In crystals, the presence of numerous ionization and recombination sites, combined with high density and periodic structure, results in more complex interference dynamics. This complexity paves the way for unique applications in generating IAPs. Using an argon (Ar) crystal as a specific example, we have proposed and theoretically demonstrated an innovative approach for generating IAPs from a solid crystal film using a multi-cycle conventional driving laser pulse.
Optimization of Low-Loss, High-Birefringence, Single-Layer, Annular, Hollow, Anti-Resonant Fiber Using a Surrogate Model-Assisted Gradient Descent Method
Authors: Zhai Lihong , Zhang Sijie , Luo Jiyang , Huang Gang , Liu Zihan
Published: December 2024

This paper proposes a novel optimization method for hollow-core, anti-resonant fiber based on a gradient descent algorithm assisted via a radial basis-function surrogate model. This approach significantly reduces the number of optimization iterations, achieving a stable improvement in birefringence performance by an order of magnitude across the operating wavelength band. Furthermore, various optimization algorithms were compared, and the indicators of their Pareto sets were analyzed to demonstrate the effectiveness of the proposed method in multi-objective optimization.
A 30 mW Laser Oscillator at 2.72 μm and 2.8 μm Wavelengths Based on Er3+-Doped Tungsten–Tellurite Fibers
Authors: Muraviev Sergei , Dorofeev Vitaly , Motorin Sergei , Koptev Maxim , Kim Arkady
Published: December 2024

The purpose of this paper was to develop fiber lasers in the 2.7–2.8 μm range based on the tungsten–tellurite glass fiber that is technically robust compared to the other fibers currently used in laser engineering. Using an advanced technology for producing ultra-dry tellurite glasses, we manufactured Er3+-doped tungsten–tellurite glass preforms with extremely low absorption and obtained active single-mode tungsten–tellurite fibers. Based on a 70 cm long fiber, we developed a laser oscillator pumped by a low-cost, high-efficiency diode laser at 976 nm. At the highest used pump power, the laser output reached 33 mW, which may be interesting for practical applications. We also measured the single-pass on/off gain of the fibers and showed that with increasing pump power amplification, as high as 5 can be reached, showing that such active fibers may also be used for increasing laser output.
Simple Design of Polarization-Selective Tunable Triple Terahertz Absorber Based on Graphene Rectangular Ring Resonator
Authors: Wang Jiang , Zhu Haixia , Ni Bo , Zhou Minhao , Feng Chengtao , Ni Haibin , Chang Jianhua
Published: December 2024

In this paper, a simple design of a polarization-selective tunable triple terahertz absorber based on a graphene rectangular ring resonator was proposed and studied. The absorber structure consists of a graphene rectangular ring resonant array on the top, SiO2 dielectric layer in the middle and gold at the bottom. The calculated results show that the absorber can achieve high-efficiency triple-band absorption under both x and y polarization incident light. When x-polarized light is incident, three distinctive absorption peaks at 2.73, 5.70 and 11.19 THz with absorption rates of 96.7%, 98.5% and 96.5% are achieved. When y-polarized light is incident, three additional absorption peaks at 2.29, 7.55 and 9.98 THz can be obtained with absorption rates of 96.3%, 90.3% and 97.4%, respectively. Moreover, the absorption wavelength of the absorber can be tuned by adjusting the chemical potential of the graphene. Owing to the high efficiency of triple-band absorption in different polarization states, the absorber has broad application prospects in terahertz polarization imaging, sensing and detection.
Temperature-Dependent Raman Scattering and Correlative Investigation of AlN Crystals Prepared Using a Physical Vapor Transport (PVT) Method
Authors: Feng Zhe Chuan , Nafisa Manika Tun , Liu Yao , Zhang Li , Wang Yingming , Xia Xiaorong , Tao Ze , Zhang Chuanwei , Yiin Jeffrey , Klein Benjamin , Ferguson Ian
Published: December 2024

Ultrawide bandgap (UWBG) AlN c- and m-face crystals have been prepared using the physical vapor transport (PVT) method and studied penetratively using temperature-dependent (TD) Raman scattering (RS) measurements under both visible (457 nm) and DUV (266 nm) excitations in 80–870 K, plus correlative atomic force microscopy (AFM) and variable-angle (VA) spectroscopic ellipsometry (SE). VASE identified their band gap energy as 6.2 eV, indicating excellent AlN characteristics and revealing Urbach energy levels of about 85 meV. Raman analyses revealed the residual tensile stress. TDRS shows that the E2(high) phonon lifetime decayed gradually in the 80–600 K range. Temperature has the greater influence on the stress of m-face grown AlN crystal. The influence of low temperature on the E2(high) phonon lifetime of m-plane AlN crystal is greater than that of the high-temperature region. By way of the LO-phonon and plasma coupling (LOPC), simulations of A1(LO) modes and carrier concentrations along different faces and depths in AlN crystals are determined. These unique and significant findings provide useful references for the AlN crystal growth and deepen our understanding on the UWBG AlN materials.
Transcriptome Proffling, Physiological and Biochemical Analyses Reveal Comprehensive Insights into Cadmium Stress in Myricaria laxiflora
Authors: Peng Yang , Yang Yu-Bing , Wang Jing-Cheng , Tian Mao-Yuan , Yuan Xing-Hai , Yang Zhi-Jiang , Zuo You-Wei , Deng Hong-Ping
Published: December 2024

With the expansion of cities and the development of industries, heavy metal pollution has caused a serious negative impact on the growth and development of animals and plants, which has become a global economic and social problem. Cadmium (Cd) is one of the main heavy metals that threaten the growth and development of plants, and it can lead to the imminent extinction of plants in severe cases. The part of upper reaches of the Yangtze River in China from Yibin to the Three Gorges Reservoir has been contaminated with varying degrees of Cd, and a rare and endangered plant called Myricaria laxiflora also lives in this area. The stress of heavy metal Cd on M. laxiflora populations is still unknown. In this study, we used the seedlings of M. laxiflora as materials, and adopted conventional physiological and biochemical analyses to characterize the morphological and physiological responses of M. laxiflora under different concentrations of Cd, and analyzed its response to Cd stress at the transcriptional level. The results showed that the wild population of M. laxiflora was stressed by the heavy metal Cd. High concentrations of Cd can inhibit the growth of M. laxiflora. M. laxiflora responded to the Cd stress through resistance substances such as malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide dismutase (SOD), catalase (CAT), and phytohormones such as auxin (IAA), gibberellin (GA) and abscisic acid (ABA). Transcriptome analysis was carried out on M. lasiflora seedlings exposed to 24 h, 48 h, and 72 h of Cd stress. Compared with 0 h (control), 2470, 11,707, and 11,733 differential expressed genes (DEGs) were identified, respectively. Among them, the number of down-regulated genes is more than the number of up-regulated genes. Transcriptome analysis showed that the upregulated genes were mainly enriched in MAPK signaling pathway, ethylene-induced pathway, ABA response pathway and other pathways, and the downregulated genes were mainly enriched in photosynthesis related pathways. Cd stress affected photosynthesis of M. laxiflora, and M. laxiflora may activate the MAPK signaling pathway through ethylene and ABA to improve the ability of Cd stress tolerance. These results reveal morphological changes, physiological and biochemical reactions and related key response pathways of M. laxiflora during Cd stress. It can provide a reference basis for habitat restoration and selection of wildlife environments for M. laxiflora.
The Dual Effect of Selenium Application in Reducing Fusarium Wilt Disease Incidence in Banana and Producing Se-Enriched Fruits
Authors: Liu Lina , Wang Chengye , Yin Kesuo , Ni Ming , Ding Yue , Li Chengyun , Zheng Si-Jun
Published: December 2024

Fusarium wilt disease severely constrains the global banana industry. The highly destructive disease is caused by Fusarium oxysporum f. sp. cubense, especially its virulent tropical race 4 (Foc TR4). Selenium (Se), a non-essential mineral nutrient in higher plants, is known to enhance plant resistance against several fungal pathogens. The experiments we conducted showed that selenium (≥10 mg/L) dramatically inhibited the growth of Foc TR4 mycelia and promoted plant growth. The further study we performed recorded a substantial reduction in the disease index (DI) of banana plants suffering from Foc TR4 when treated with selenium. The selenium treatments (20~160 mg/L) demonstrated significant control levels, with recorded symptom reductions ranging from 42.4% to 65.7% in both greenhouse and field trials. The DI was significantly negatively correlated with the total selenium content (TSe) in roots. Furthermore, selenium treatments enhanced the antioxidant enzyme activities of peroxidase (POD), polyphenol oxidase (PPO), and glutathione peroxidase (GSH-Px) in banana. After two applications of selenium (100 and 200 mg/plant) in the field, the TSe in banana pulps increased 23.7 to 25.9-fold and achieved the Se enrichment standard for food. The results demonstrate that selenium applications can safely augment root TSe levels, both reducing Fusarium wilt disease incidence and producing Se-enriched banana fruits. For the first time, this study has revealed that selenium can significantly reduce the damage caused by soil-borne pathogens in banana by increasing the activities of antioxidant enzymes and inhibiting fungal growth.
Detecting Sensitive Spectral Bands and Vegetation Indices for Potato Yield Using Handheld Spectroradiometer Data
Authors: Gomez Diego , Salvador Pablo , Rodrigo Juan Fernando , Gil Jorge
Published: December 2024

Remote sensing is a valuable tool in precision agriculture due to its spatial and temporal coverage, non-destructive method of data collection, and cost-effectiveness. In this study, we measured the canopy reflectance of potato (Solanum tuberosum L.) crops on a plant-by-plant basis with a handheld spectrometer instrument. Our study pursues two primary objectives: (1) determining the optimal temporal aggregation for measuring canopy signals related to potato yield and (2) identifying the best spectral bands in the 350–2500 nm domain and vegetation indices. The study was conducted over two consecutive years (2020 and 2021) with 60 plants per plot, encompassing six potato varieties and three replicates annually throughout the growth season. Employing correlation analysis and dimensionality reduction, we identified 23 independent features significantly correlated with tuber yield. We used multiple linear regression analysis to model the relationship between the selected features and yield and to compare their influence in the fitted model. We used the Leave-One-Out Cross-Validation (LOOCV) method to assess the validity of the model (RMSE = 702 g and %RMSE = 29.2%). The most significant features included the Gitelson2 and Vogelmann indices. The optimal time period for measurements was determined to be from 56 to 100 days after planting. These findings may contribute to the advancement of precision farming by proposing tailored sensor applications, paving the way for improved agricultural practices and enhanced food security.
Emerging Diseases in Spain Strawberry Crops: Neopestalotiopsis Leaf and Crown Rot and Fusarium Wilt
Authors: Avilés Manuel , Pastrana Ana M. , Borrero Celia
Published: December 2024

In recent years, strawberry cultivation in Spain has been increasingly affected by new and re-emerging fungal diseases. The most significant emerging diseases in Spain include those caused by Neopestalotiopsis spp. Maharachch., K.D.Hyde & Crous and Fusarium oxysporum f. sp. fragariae Winks & Y.N. Williams. These pathogens are difficult to control due to their pathogenic variability (presence of pathotypes and/or races), the lack of knowledge about the susceptibility of the different cultivars, the limited availability of effective fumigants, and the absence of sufficient information about their sources of inoculum. Both pathogens can cause root and crown rot, leading to plant collapse and significant losses for strawberry producers. Several factors have contributed to the rise of these diseases in Spain: (i) the gradual ban on key soil fumigants has left the crop vulnerable; (ii) there has been a notable diversification in the origin of mother plants used in cultivation, with plants now sourced from various countries, increasing the risk of long-distance pathogen spread; (iii) the introduction of numerous new strawberry varieties, which exposes more genotypes to pathogenic infections; and (iv) changes in planting times, leading to younger and more vulnerable plants being exposed to heat stress, as well as an increase in disease susceptibility. Neopestalotiopsis spp. and Fusarium oxysporum f. sp. fragariae have also become major threats to strawberry crops worldwide, spreading through nursery plants and the movement of plant material. The latest research findings in Spain on both pathogens are highlighted in this manuscript.
Transcriptomic Analysis of the CAM Species Kalanchoë fedtschenkoi Under Low- and High-Temperature Regimes
Authors: Hu Rongbin , Zhang Jin , Jawdy Sara , Sreedasyam Avinash , Lipzen Anna , Wang Mei , Ng Vivian , Daum Christopher , Keymanesh Keykhosrow , Liu Degao , Hu Alex , Chen Jin-Gui , Tuskan Gerald A. , Schmutz Jeremy , Yang Xiaohan
Published: December 2024

Temperature stress is one of the major limiting environmental factors that negatively impact global crop yields. Kalanchoë fedtschenkoi is an obligate crassulacean acid metabolism (CAM) plant species, exhibiting much higher water-use efficiency and tolerance to drought and heat stresses than C3 or C4 plant species. Previous studies on gene expression responses to low- or high-temperature stress have been focused on C3 and C4 plants. There is a lack of information about the regulation of gene expression by low and high temperatures in CAM plants. To address this knowledge gap, we performed transcriptome sequencing (RNA-Seq) of leaf and root tissues of K. fedtschenkoi under cold (8 °C), normal (25 °C), and heat (37 °C) conditions at dawn (i.e., 2 h before the light period) and dusk (i.e., 2 h before the dark period). Our analysis revealed differentially expressed genes (DEGs) under cold or heat treatment in comparison to normal conditions in leaf or root tissue at each of the two time points. In particular, DEGs exhibiting either the same or opposite direction of expression change (either up-regulated or down-regulated) under cold and heat treatments were identified. In addition, we analyzed gene co-expression modules regulated by cold or heat treatment, and we performed in-depth analyses of expression regulation by temperature stresses for selected gene categories, including CAM-related genes, genes encoding heat shock factors and heat shock proteins, circadian rhythm genes, and stomatal movement genes. Our study highlights both the common and distinct molecular strategies employed by CAM and C3/C4 plants in adapting to extreme temperatures, providing new insights into the molecular mechanisms underlying temperature stress responses in CAM species.
Exploring Imaging Techniques for Detecting Tomato Spotted Wilt Virus (TSWV) Infection in Pepper (Capsicum spp.) Germplasms
Authors: Mensah Eric Opoku , Oh Hyeonseok , Song Jiseon , Baek Jeongho
Published: December 2024

Due to the vulnerability of pepper (Capsicum spp.) and the virulence of tomato spotted wilt virus (TSWV), seasonal shortages and surges of prices are a challenge and thus threaten household income. Traditional bioassays for detecting TSWV, such as observation for symptoms and reverse transcription-PCR, are time-consuming, labor-intensive, and sometimes lack precision, highlighting the need for a faster and more reliable approach to plant disease assessment. Here, two imaging techniques—Red–Green–Blue (RGB) and hyperspectral imaging (using NDVI and wavelength intensities)—were compared with a bioassay method to study the incidence and severity of TSWV in different pepper accessions. The bioassay results gave TSWV an incidence from 0 to 100% among the accessions, while severity ranged from 0 to 5.68% based on RGB analysis. The normalized difference vegetative index (NDVI) scored from 0.21 to 0.23 for healthy spots on the leaf but from 0.14 to 0.19 for disease spots, depending on the severity of the damage. The peak reflectance of the disease spots on the leaves was identified in the visible light spectrum (430–470 nm) when spectral bands were studied in the broad spectrum (400.93–1004.5 nm). For the selected wavelength in the visible light spectrum, a high reflectance intensity of 340 to 430 was identified for disease areas, but between 270 and 290 for healthy leaves. RGB and hyperspectral imaging techniques can be recommended for precise and accurate detection and quantification of TSWV infection.
Screening High-Biomass Grasses for Cadmium Phytoremediation
Authors: Dias Olívia Bibiana Souza , Borgo Lucélia , Silva Deivisson Ferreira da , Souza Alisson de Carli , Tezotto Tiago , Vangronsveld Jaco , Guilherme Luiz Roberto Guimarães , Rabêlo Flávio Henrique Silveira
Published: December 2024

Investigating the ability of non-hyperaccumulator plants to grow in soils polluted by cadmium (Cd) and their potential for phytostabilization or phytoextraction is essential for assessing their use in phytomanagement efficiency. Therefore, we evaluated the tolerance of high-biomass grasses to Cd by measuring biomass production and element accumulation and valued them for their suitability for phytoextraction or phytostabilization purposes on moderately Cd-polluted land (total Cd concentration of 7.5 mg kg−1) by determining Cd accumulation in the plants and calculating the bioconcentration (Cd BCF) and translocation factors (Cd TF). Among the ten species under investigation, Panicum maximum cv. Massai and Pennisetum glaucum cv. Purpureum Schum showed lower root biomass due to Cd exposure. Cadmium exposure altered element accumulation in some grass species by reducing P, K, and Mg accumulation in P. glaucum cv. Purpureum Schum; K accumulation in P. maximum cv. Massai; Mg accumulation in P. maximum cv. Mombaça; Ca, Fe, and Zn accumulation in P. maximum cv. Aruana; and B accumulation in Brachiaria brizantha cv. Piatã. However, this was not correlated with lowered biomass production, except for K, which was associated with lowered root biomass allocation in P. maximum cv. Massai and P. glaucum cv. Purpureum Schum. Cadmium concentrations decreased from roots to shoots, indicating a clear limitation of upward Cd transport. Although some grasses exhibited a Cd BCF > 1, the Cd TF remained below 0.4 for all tested species. These results indicate that, under moderate Cd pollution, the evaluated grasses are more suitable for Cd phytostabilization than phytoextraction, except for P. maximum cv. Massai and P. glaucum cv. Purpureum Schum, which showed inhibited root growth and may not be efficient over time.
Alternative Splicing of PheNAC23 from Moso Bamboo Impacts Flowering Regulation and Drought Tolerance in Transgenic Arabidopsis
Authors: Xie Lihua , Li Xiangyu , Yao Pengqiang , Cheng Zhanchao , Cai Miaomiao , Liu Chunyang , Wang Zhe , Gao Jian
Published: December 2024

NAC (NAM, ATAF, and CUC) transcription factors are essential in regulating plant stress response and senescence, with their functions being modulated by alternative splicing. The molecular mechanisms of stress-induced premature flowering and drought tolerance in Phyllostachys edulis (moso bamboo) are not yet fully understood. In this study, a novel NAC variant derived from PheNAC23, named PheNAC23ES, was isolated. PheNAC23ES exhibited distinct expression patterns compared to PheNAC23 during leaf senescence and drought stress response. Overexpression of PheNAC23 promoted flowering and reduced its tolerance to drought stress in Arabidopsis thaliana (A. thaliana). However, overexpression of PheNAC23ES exhibited the opposite functions. PheNAC23 was localized in the nucleus and had transactivation activity, while PheNAC23ES had a similar localization to the control green fluorescent protein and no transactivation activity. Further functional analysis revealed that PheNAC23ES could interact with PheNAC23, suggesting that PheNAC23ES might serve as a small interfering peptide that affects the function of PheNAC23 by binding to it.
Effect of Biopesticide Novochizol on Development of Stem Rust Puccinia graminis f. sp. tritici in Wheat, T. aestivum L.
Authors: Shcherban Andrey B. , Skolotneva Ekaterina S. , Fedyaeva Anna V. , Boyko Natalya I. , Fomenko Vladislav V.
Published: December 2024

The use of biological plant protection products is promising for agriculture. In particular, chitosan-based biopesticides have become widespread for stimulating growth and protecting plants from a wide range of pathogens. Novochizol is a product obtained by intramolecular cross-linking of linear chitosan molecules and has a globular shape, which provides it with a number of advantages over chitosan. Novochizol has previously been shown to have a stimulating effect on the growth and development of common wheat (Triticum aestivum L.). However, the effect of this preparation on the protective mechanisms against rust diseases has not been studied before. Our studies have revealed the dose effect of the preparation on the development of stem rust of wheat. When treating plants with novochizol at a concentration of 0.125% four days before infection, the best results were obtained, namely: a stable reaction was observed and the number of pustules decreased. To identify critical points of the drug’s effect on the protective mechanism against stem rust, we used an adrenaline test, which allows for a quick assessment of the pro/antioxidant status of plant extracts. We also assessed the activity of the major antioxidant enzymes, peroxidase and catalase, using commercial kits and the Folin–Ciocalteu reaction to assess the concentration of phenolic compounds. As a result, two stages were identified in infected plants pretreated with novochizol: early (up to 10 h after inoculation), characterized by antioxidant activity, and late (10–244 h), with prooxidant activity. These stages correspond to two peaks of accumulation of reactive oxygen species (ROS) in response to pathogen infection. The first peak is associated with the accumulation of superoxide anion O2−, which is converted into oxygen and hydrogen peroxide under the action of the enzyme SOD (superoxide dismutase). The second peak is associated with the accumulation of H2O2. Hydrogen peroxide performs a protective function leading to the death of pathogen mycelial cells. In comparison with infected plants without novochizol treatment, we found a decrease in the activity of catalase (an enzyme that breaks down H2O2) at both stages, as well as peroxidase in the interval from 10 to 144 h after inoculation. Also, an increase in the concentration of phenolic compounds was found in the treated infected plants. We suggest that these changes under the influence of pretreatment with novochizol contribute to enhancements in plant defense functions against stem rust. Taking into account the physicochemical advantages of novochizol over chitosan, which provide a very low effective dose of the drug, the obtained results indicate its promise and safety as a biological plant protection product. This work is a preliminary stage for an extended analysis of the effect of novochizol on plant immunity using biochemical and molecular genetic approaches.
Preparation and Performance Analysis of Tung Cake Protein Adhesive
Authors: Wang Wei , Zheng Ke , Zhao Wenzheng , Zheng Shenglong , Wan Hui , Gao Jingran
Published: December 2024

Tung oil pressing generates a substantial amount of tung cake waste rich in protein, which can be used to develop a novel wood protein adhesive. This study determined the optimal alkali treatment parameters based on NaOH concentration, reaction temperature, and reaction time. Potassium permanganate (KMnO4) and methyl trimethoxy silane (MTMS) were then sequentially added for cross-linking modification to achieve the optimal preparation process for the tung cake protein adhesive. Bonding strength was tested on pressed boards, and various characterization techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TG/TGA), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM), were used. The results indicated the following: (1) Optimal preparation conditions: The best preparation process for the adhesive involved 30% NaOH at 50 °C for 50 min, with the addition of 12% KMnO4 and 6% MTMS, meeting Class II plywood standards. (2) XRD and FTIR analyses revealed that carbohydrates in the tung cake oxidized and reacted with protein amino groups. The active groups in the protein cross-linked with MTMS, forming a spatial network structure, reducing hydrophilic groups, and enhancing water resistance. (3) TG/TGA and DSC showed that the thermal stability of the modified adhesive improved, thermogravimetric loss was reduced, and curing performance was enhanced. (4) SEM verified the adhesive’s reaction mechanism, demonstrating that MTMS filled the protein structure unfolded by KMnO4, forming a three-dimensional network and improving bonding strength. This study successfully developed a new, formaldehyde-free, environmentally friendly tung cake protein adhesive with excellent performance.
A UV-Protective Textile Coating Based on Recycled Poly(vinyl butyral) (PVB): A New Life for a Waste Polymer
Authors: Cei Noemi , Canesi Ilaria , Nejrotti Stefano , Montalbano Giorgia , Darjazi Hamideh , Piovano Alessandro , Bonomo Matteo , Fina Alberto , Yecora Beatriz , Perez Angelica , Barolo Claudia , Gerbaldi Claudio , Spinelli Daniele
Published: December 2024

Polyvinyl butyral (PVB) is a high-performance thermoplastic polymer, commonly used as an interlayer material in laminated safety glass for the automotive and architectural sectors. Currently, there is no end-of-life cycle program for a substantial amount of PVB film, which mainly ends up in landfills. According to a circular approach, PVB can be revalorized after efficient separation and recovery from glass. Thus, the aim of this work was to develop functional coatings for textile applications using recycled PVB (re-PVB), also in combination with an organic UV absorber, to enable the production of UV-protective final coated fabrics. The re-PVB-coated fabrics were obtained through an industrially scalable spraying process (leading to an average weight increase of 20 ± 3 wt.%), and the effectiveness of the application was evaluated according to different characterization techniques, such as FT-IR (Fourier transform–infrared) spectroscopy, SEM (scanning electron microscope), a washing test, a mechanical test, a thermo-physiological test, and the ultraviolet protection factor (UPF). Based on the results, the re-PVB-coated fabrics appeared stable upon washing (with a negligible weight loss compared to the average amount of coating) and effective in UV protection (with a final UPF being four times higher and a reduced UVA transmittance from 2.0% to 0.6%).
Influence of Lipid Fermentation Wastewater on Yield and Nutritional Profile of Edible and Medicinal Mushrooms
Authors: Melanouri Eirini-Maria , Diamantis Ilias , Papanikolaou Seraphim , Diamantopoulou Panagiota
Published: December 2024

Utilizing agricultural waste to produce mushrooms may be a cost-effective and environmentally friendly proposition to address the nutritional and health demands of the growing global population. Mushrooms can grow on a range of substrates and their selection is based on their availability and cost. In this study, five types of local waste were mixed: olive crop residues (OC), coffee residue (CR) or rice husk (RH) with wheat straw (WS) and beech wood shavings (BW), respectively. Then, the mixtures were sprayed with 20% w/w lipid fermentation wastewater (LFW) from Rodosporidium toruloides that was used as an alternative substrate-moistening method. Afterwards, these mixtures were tested for cultivating Pleurotus spp., Ganoderma spp. and Lentinula edodes. The results showed that the substrate significantly affected the incubation period and the biological efficiency (BE), with OC mixed substrates proving to be the most favorable across the different species. Pleurotus spp. had the shortest cultivation times and the highest BE, while G. lucidum required the longest incubation periods and had the lowest BE, particularly on CR substrates. The study also found that substrates affected mushroom morphology. Nutritional analysis revealed significant differences in protein, polysaccharides, lipids, ash and energy content, depending on the species and substrate. High protein levels were found in P. eryngii (28.05–29.58% d.w.) and G. resinaceum (28.71–29.90% d.w.). The elevated total phenolic compounds (28.47–40.17 mgGAE/g) values in carposomes from CR and OC substrates for Ganoderma spp., L. edodes, P. pulmonarius and P. ostreatus, along with antioxidant activity (DPPH, ABTS, FRAP) assays, highlighted the crucial role of substrate composition in enhancing the medicinal properties of mushrooms. The mixed substrates also influenced the fatty acid (FA) and polysaccharide composition, with WS increasing unsaturated FAs and glucose (<69.8%) being the primary monosaccharide. The study suggests that using the spraying method of 20% w/w LFW as a moisture agent in these substrates is effective for mushroom production.
Pre-Commercial Demonstration of a Photosynthetic Upgrading Plant: Investment and Operating Cost Analysis
Authors: Ruiz Palomar César , García Álvaro Alfonso , Muñoz Raúl , Repáraz Carlos , Ortega Marcelo F. , de Godos Ignacio
Published: December 2024

Pig farms have been identified as one of the most important sources of greenhouse gas emissions. This study demonstrates the production of vehicle biomethane in a demonstration prototype plant based on photosynthetic upgrading technology, where the CO2 and H2S present in biogas are consumed by a microalgae culture. The information collected during the prototype construction allowed for an assessment of the capital and operating costs of this novel biogas upgrading technology with other conventional systems. With this objective, the costs of the equipment comprising the biogas cleaning and purification system were calculated considering a biogas flow rate of 5 m3 h−1, corresponding to a small–medium biogas plant and an average pig farm size. The sustainability and competitiveness of the algae upgrading system and the low capital and operating costs vis à vis other upgrading technologies were proven. With a net energy production of 687 kWh day−1 and an annual profit of €30,348 in a 3500 head pig farm, this technology can be easily installed in livestock farms, increasing the benefits and reducing the carbon footprint.