bluuebunny/crossref_metadata_2025 huggingface dataset:
Photomediated Larvicidal Activity of Pheophorbide a against Cercaria Larvae of Fasciola gigantica
Authors: Singh Divya Jyoti , Singh Vinay Kumar , Singh D. K.
Published: January 2017

Fasciolosis is a parasitic disease caused by Fasciola gigantica. The freshwater snail Lymnaea acuminata is the intermediate host of F. gigantica which cause endemic fasciolosis in the northern part of India. To investigate larvicidal activity of pure and laboratory extracted pheophorbide a (Pa) against cercaria larvae of F. gigantica, data were analyzed in different spectra of visible light, sunlight, and laboratory conditions. Photostimulation of chlorophyll derivative pheophorbide a (Pa) caused time and concentration dependent larvicidal activity against cercaria larvae of F. gigantica. Larvicidal activity of pure Pa under 650 nm and 400–650 nm (8 h LC50 0.006 mg/10 mL) was more pronounced than extracted Pa under same irradiations (650 nm LC50 0.12 mg/10 mL, 400–650 nm LC50 0.14 mg/10 mL). Lowest toxicity of pure (8 h LC50 0.14 mg/10 mL) and extracted Pa (8 h LC50 1.25 mg/10 mL) was noted under 400 nm. Pa was found to be toxic in laboratory conditions also. The results presented in this paper indicate that pheophorbide a possess potential larvicidal activity against Fasciola gigantica larvae in different wavelengths of visible light, sunlight, and laboratory conditions.
Mechanical Properties of Steel-FRP Composite Bars under Tensile and Compressive Loading
Authors: Sun Zeyang , Tang Yu , Luo Yunbiao , Wu Gang , He Xiaoyuan
Published: January 2017

The factory-produced steel-fiber reinforced polymer composite bar (SFCB) is a new kind of reinforcement for concrete structures. The manufacturing technology of SFCB is presented based on a large number of handmade specimens. The calculated stress-strain curves of ordinary steel bar and SFCB under repeated tensile loading agree well with the corresponding experimental results. The energy-dissipation capacity and residual strain of both steel bar and SFCB were analyzed. Based on the good simulation results of ordinary steel bar and FRP bar under compressive loading, the compressive behavior of SFCB under monotonic loading was studied using the principle of equivalent flexural rigidity. There are three failure modes of SFCB under compressive loading: elastic buckling, postyield buckling, and no buckling (ultimate compressive strength is reached). The increase in the postyield stiffness of SFCB rsf can delay the postyield buckling of SFCB with a large length-to-diameter ratio, and an empirical equation for the relationship between the postbuckling stress and rsf is suggested, which can be used for the design of concrete structures reinforced by SFCB to consider the effect of reinforcement buckling.
Effects of α-ZrP on Crystallinity and Flame-Retardant Behaviors of PA6/MCA Composites
Authors: Xiao Yuanfang , Xu Jiayou , Huang Siwen , Deng Haiming
Published: January 2017

Novel flame-retardant Polyamide6/Melamine cyanurate-alpha-zirconium phosphate (PA6/MCA-α-ZrP) composites were prepared via the incorporation of the modified MCA (MCA-α-ZrP) into PA6. MCA-α-ZrP were synthesized through the self-assembly of α-ZrP, Melamine (ME), and cyanuric acid (CA) molecules. The results of differential scanning calorimetry (DSC) and X-ray diffraction (XRD) showed that the incorporation of enough α-ZrP (30 wt% MCA) caused the increased crystallinity of PA6 and tended to form γ phase. The thermogravimetric analysis (TGA) and heat distortion temperature (HDT) test illustrated that the α-ZrP could increase the residue and HDT values of PA6/MCA and showed a synergistic effect with MCA. The combination of MCA and α-ZrP caused the enhancement of vertical burning test (UL-94) rating. Cone calorimeter test (CCT) gave clear evidences that PA6/MCA-10α-ZrP composites with low heat release rate (HRR), low total heat release (THR), and high amounts of char residues after combustion compared with PA6/MCA and PA6/MCA-30α-ZrP. What is more, excellent mechanical properties were kept even though MCA and α-ZrP were dispersed not as good as expected.
CACNA1B (Cav2.2) Overexpression and Its Association with Clinicopathologic Characteristics and Unfavorable Prognosis in Non-Small Cell Lung Cancer
Authors: Zhou Xiaoyu , Wang Wei , Zhang Shu , Wang Xudong , Tang Zhiyuan , Gu Jun , Li Jun , Huang Jianan
Published: January 2017

CACNA1B (Cav2.2) encodes an N-type voltage-gated calcium channel (VGCC) ubiquitously expressed in brain and peripheral nervous system that is important for regulating neuropathic pain. Because intracellular calcium concentration is a key player in cell proliferation and apoptosis, VGCCs are implicated in tumorigenesis. Recent studies have identified CACNA1B (Cav2.2) being overexpressed in prostate and breast cancer tissues when compared to adjacent normal tissues; however, its role in non-small cell lung cancer (NSCLC) has not been investigated. In this study, we determined the mRNA and protein expression of CACNA1B (Cav2.2) in NSCLC tumorous and adjacent nontumorous tissues by quantitative reverse transcription PCR (qRT-PCR) and tissue microarray immunohistochemistry analysis (TMA-IHC), respectively. CACNA1B (Cav2.2) protein expressions in tumorous tissues were correlated with NSCLC patients’ clinical characteristics and overall survival. CACNA1B (Cav2.2) mRNA and protein expression levels were higher in NSCLC tumorous tissues than in nontumorous tissues. High CACNA1B (Cav2.2) protein expression was associated with higher TNM stages, and CACNA1B (Cav2.2) protein expression is an independent prognostic marker in NSCLC. Based on our results, we conclude that CACNA1B (Cav2.2) plays a role in NSCLC development and progression. Elucidating the underlying mechanism may help design novel treatment by specifically targeting the calcium regulation pathway for NSCLC, a devastating disease with increasing incidence and mortality in China.
Numerical Simulation of Surface Energy and Water Balances over a Semiarid Grassland Ecosystem in the West African Savanna
Authors: Quansah Emmanuel , Katata Genki , Mauder Matthias , Annor Thompson , Amekudzi Leonard K. , Bliefernicht Jan , Heinzeller Dominikus , Balogun Ahmed A. , Kunstmann Harald
Published: January 2017

To understand surface energy exchange processes over the semiarid regions in West Africa, numerical simulations of surface energy and water balances were carried out using a one-dimensional multilayer atmosphere-SOil-VEGetation (SOLVEG) model for selected days of the dry and rainy seasons over a savanna grassland ecosystem in Sumbrungu in the Upper East region of Ghana. The measured Bowen ratio was used to partition the residual energy into the observed sensible heat flux (H) and latent heat flux (LE) in order to investigate the impact of the surface energy closure on model performance. The results showed that the model overall reproduced the diurnal changes in the observed energy fluxes, especially the net radiation (Rn), compared to half-hourly eddy covariance flux measurements, for the study periods. The performance measure in terms of the correlation coefficient (R), centred root mean square error (RMSE), and normalized standard deviation (σ) between the simulated H and LE and their corresponding uncorrected observed values ranged between R = 0.63–0.99 and 0.83–0.94, RMSE = 0.88–1.25 and 0.88–1.92, and σ = 0.95–2.23 and 0.13–2.82 for the dry and rainy periods respectively, indicating a moderate to good model performance. The partitioning of H and LE by SOLVEG was generally in agreement with the observations during the dry period but showed clear discrepancies during the rainy period, particularly after rainfall events. Further sensitivity tests over longer simulation periods (e.g., 1 year) are required to improve model performance and to investigate seasonal exchanges of surface energy fluxes over the West African Savanna ecosystems in more details.
A New Vortex Initialization Scheme Coupled with WRF-ARW
Authors: Fung Jimmy Chi Hung , Gao Guangze
Published: January 2017

The ability of numerical simulations to predict typhoons has been improved in recent decades. Although the track prediction is satisfactory, the intensity prediction is still far from adequate. Vortex initialization is an efficient method to improve the estimations of the initial conditions for typhoon forecasting. In this paper, a new vortex initialization scheme is developed and evaluated. The scheme requires only observational data of the radius of maximum wind and the max wind speed in addition to the global analysis data. This scheme can also satisfy the vortex boundary conditions, which means that the vortex is continuously merged into the background environment. The scheme has a low computational cost and has the flexibility to adjust the vortex structure. It was evaluated with 3 metrics: track, center sea-level pressure (CSLP), and maximum surface wind speed (MWSP). Simulations were conducted using the WRF-ARW numerical weather prediction model. Super and severe typhoon cases with insufficiently strong initial MWSP were simulated without and with the vortex initialization scheme. The simulation results were compared with the 6-hourly observational data from Hong Kong Observatory (HKO). The vortex initialization scheme improved the intensity (CSLP and MWSP) prediction results. The scheme was also compared with other initialization methods and schemes.
A Table Lookup Method for Exact Analytical Solutions of Nonlinear Fractional Partial Differential Equations
Authors: Juan-Juan Ji , Ye-Cai Guo , Lan-Fang Zhang , Chao-Long Zhang
Published: January 2017

A table lookup method for solving nonlinear fractional partial differential equations (fPDEs) is proposed in this paper. Looking up the corresponding tables, we can quickly obtain the exact analytical solutions of fPDEs by using this method. To illustrate the validity of the method, we apply it to construct the exact analytical solutions of four nonlinear fPDEs, namely, the time fractional simplified MCH equation, the space‐time fractional combined KdV‐mKdV equation, the (2 + 1)‐dimensional time fractional Zoomeron equation, and the space‐time fractional ZKBBM equation. As a result, many new types of exact analytical solutions are obtained including triangular periodic solution, hyperbolic function solution, singular solution, multiple solitary wave solution, and Jacobi elliptic function solution.
Absorbed Pb2+ and Cd2+ Ions in Water by Cross-Linked Starch Xanthate
Authors: Feng Kai , Wen Guohua
Published: January 2017

A cross-linked starch xanthate was prepared by graft copolymerization of acrylamide and sodium acrylate onto starch xanthate using potassium persulfate and sodium hydrogen sulfite initiating system and N,N′-methylenebisacrylamide as a cross-linker. As this kind of cross-linked potato starch xanthate can effectively absorb heavy metal ions, it was dispersed in aqueous solutions of divalent heavy metal ions (Pb2+ and Cd2+) to investigate their absorbency by the polymer. Factors that can influence absorbency were investigated, such as the ratio of matrix to monomers, the amount of initiator and cross-linker, pH, and the concentration of metal ions. Results were reached and conclusion was drawn that the best synthetic conditions for the polymer adsorbing Pb2+ and Cd2+ were as follows: the quality ratio of matrix to monomers was 1 : 12 and 1 : 11, the amount of initiator was 2.4% and 3.2% of matrix, and the amount of cross-linker was 12 mg and 13 mg. When the initial concentration of ions was 10 mg/L, the highest quantities of adsorption of Pb2+ and Cd2+ were 47.11 mg/g and 36.55 mg/g. Adsorption mechanism was discussed by using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM), Energy Dispersive X-Ray Spectroscopy (EDS) test, and adsorption kinetic simulation.
A Group Decision‐Making Model Based on Regression Method with Hesitant Fuzzy Preference Relations
Authors: Xue Min , Du Yifei
Published: January 2017

In recent years, the decision‐making models with hesitant fuzzy preference relations (HFPRs) have received a lot of attention by some researchers. Meanwhile, the previous studies normally adopt normalization technical means to ensure the same number for all elements, which biases original information of decision‐makers. In order to overcome this problem, in this paper, the multiplicative consistency of HFPRs is defined and the highest consistent reduced HFPRs are obtained by means of fuzzy linear programming method from given HFPRs. The proposed regression method eliminates the unreasonable information and retains the reasonable information from a given HFPR. In addition, the proposed method overcomes drawbacks of Zhu and Xu’s regression method and is more simple and effective. On account of the obtained reduced HFPRs by the proposed regression method, a GDM model is established. Finally, a supplier selection problem was researched to present the effectiveness and pragmatism of the proposed approach, which proved that the method could offer beneficial insights into the GDM procedure.
Desensitization: Overcoming the Immunologic Barriers to Transplantation
Authors: Sethi Supreet , Choi Jua , Toyoda Mieko , Vo Ashley , Peng Alice , Jordan Stanley C.
Published: January 2017

HLA (Human Leucocyte Antigen) sensitization is a significant barrier to successful kidney transplantation. It often translates into difficult crossmatch before transplant and increased risk of acute and chronic antibody mediated rejection after transplant. Over the last decade, several immunomodulatory therapies have emerged allowing for increased access to kidney transplantation for the immunologically disadvantaged group of HLA sensitized end stage kidney disease patients. These include IgG inactivating agents, anti-cytokine antibodies, costimulatory molecule blockers, complement inhibitors, and agents targeting plasma cells. In this review, we discuss currently available agents for desensitization and provide a brief analysis of data on novel biologics, which will likely improve desensitization outcomes, and have potential implications in treatment of antibody mediated rejection.
Hemadsorption with Adult CytoSorb® in a Low Weight Pediatric Case
Authors: Cirstoveanu Catalin Gabriel , Barascu Ileana , Mc Kenzie Stancu Samantha
Published: January 2017

Cytokine adsorber (CytoSorb) has been used successfully as adjunctive treatment for adult patients with elevated cytokine levels in the setting with severe sepsis and septic shock and to reduce blood myoglobin, unconjugated bilirubin, and conjugated bilirubin. In this article we present the case of a nine-month-old male infant who was admitted to the NICU due to sepsis after cardiac surgery, Fallot tetralogy, and multisystem organ failure (MSOF) including liver failure and renal failure which was successfully treated by a combination of continuous hemodiafiltration (HDF) and hemadsorption with CytoSorb. HDF was safe and effective from the first day for urea removal, but the patient’s bilirubin levels kept increasing gradually, culminating on the 9th day with a maximum value of 54 mg/dL of total bilirubin and 31.67 mg/dL of direct bilirubin when we performed hemadsorption with CytoSorb. Over the 49-hour period of hemadsorption, the total bilirubin value decreased from 54 to 14 mg/dL, and the patient’s general status improved considerably accompanied by a rapid drop of aminotransferases. Hemodynamic status has been improved as well and inotropes dropped rapidly. The patient’s ventilation settings improved during CytoSorb treatment permitting weaning the patient from mechanical ventilation after five days of hemadsorption. The patient was discharged home after 34 days of hospitalization, in a good general status.
Amyloid β Peptide-Induced Changes in Prefrontal Cortex Activity and Its Response to Hippocampal Input
Authors: Flores-Martínez Ernesto , Peña-Ortega Fernando
Published: January 2017

Alterations in prefrontal cortex (PFC) function and abnormalities in its interactions with other brain areas (i.e., the hippocampus) have been related to Alzheimer Disease (AD). Considering that these malfunctions correlate with the increase in the brain’s amyloid beta (Aβ) peptide production, here we looked for a causal relationship between these pathognomonic signs of AD. Thus, we tested whether or not Aβ affects the activity of the PFC network and the activation of this cortex by hippocampal input stimulation in vitro. We found that Aβ application to brain slices inhibits PFC spontaneous network activity as well as PFC activation, both at the population and at the single-cell level, when the hippocampal input is stimulated. Our data suggest that Aβ can contribute to AD by disrupting PFC activity and its long-range interactions throughout the brain.
An Algorithm: Optimal Homotopy Asymptotic Method for Solutions of Systems of Second‐Order Boundary Value Problems
Authors: Mufti Muhammad Rafiq , Qureshi Muhammad Imran , Alkhalaf Salem , Iqbal S.
Published: January 2017

Optimal homotopy asymptotic method (OHAM) is proposed to solve linear and nonlinear systems of second‐order boundary value problems. OHAM yields exact solutions in just single iteration depending upon the choice of selecting some part of or complete forcing function. Otherwise, it delivers numerical solutions in excellent agreement with exact solutions. Moreover, this procedure does not entail any discretization, linearization, or small perturbations and therefore reduces the computations a lot. Some examples are presented to establish the strength and applicability of this method. The results reveal that the method is very effective, straightforward, and simple to handle systems of boundary value problems.
Metastatic Crohn’s Disease: An Approach to an Uncommon but Important Cutaneous Disorder
Authors: Aberumand Babak , Howard Jessica , Howard John
Published: January 2017

Objective. To provide physicians with a clinical approach to metastatic Crohn’s disease (MCD).Main Message. Metastatic Crohn’s disease, defined as skin lesions present in areas noncontiguous with the gastrointestinal tract, is the rarest cutaneous manifestation of Crohn’s disease. MCD lesions vary in morphology and can arise anywhere on the skin. MCD presents equally in both sexes and across age groups. Cutaneous findings may precede, develop concurrently with, or follow gastrointestinal involvement. A detailed history and thorough physical examination including a full-skin exam may help to exclude other dermatoses, as MCD can mimic other common disorders. A biopsy is required for a definitive diagnosis. Treatment options for MCD remain underwhelming due to the lack of randomized control studies and varying responses of reported therapeutic methods. Topical, intralesional, and systemic corticosteroids, antibiotics, traditional immunosuppressants, and surgery have shown mixed results. Recently, biologics have shown promise, even with refractory cases of MCD.Conclusion. MCD is an important cutaneous manifestation of this inflammatory disorder. Although a rare entity, early recognition can provide opportunity for successful therapeutic intervention.
A Hybrid Classification System for Heart Disease Diagnosis Based on the RFRS Method
Authors: Liu Xiao , Wang Xiaoli , Su Qiang , Zhang Mo , Zhu Yanhong , Wang Qiugen , Wang Qian
Published: January 2017

Heart disease is one of the most common diseases in the world. The objective of this study is to aid the diagnosis of heart disease using a hybrid classification system based on the ReliefF and Rough Set (RFRS) method. The proposed system contains two subsystems: the RFRS feature selection system and a classification system with an ensemble classifier. The first system includes three stages: (i) data discretization, (ii) feature extraction using the ReliefF algorithm, and (iii) feature reduction using the heuristic Rough Set reduction algorithm that we developed. In the second system, an ensemble classifier is proposed based on the C4.5 classifier. The Statlog (Heart) dataset, obtained from the UCI database, was used for experiments. A maximum classification accuracy of 92.59% was achieved according to a jackknife cross-validation scheme. The results demonstrate that the performance of the proposed system is superior to the performances of previously reported classification techniques.
Sterile Neuroinflammation and Strategies for Therapeutic Intervention
Authors: Banjara Manoj , Ghosh Chaitali
Published: January 2017

Sterile neuroinflammation is essential for the proper brain development and tissue repair. However, uncontrolled neuroinflammation plays a major role in the pathogenesis of various disease processes. The endogenous intracellular molecules so called damage-associated molecular patterns or alarmins or damage signals that are released by activated or necrotic cells are thought to play a crucial role in initiating an immune response. Sterile inflammatory response that occurs in Alzheimer’s disease (AD), Parkinson’s disease (PD), stroke, hemorrhage, epilepsy, or traumatic brain injury (TBI) creates a vicious cycle of unrestrained inflammation, driving progressive neurodegeneration. Neuroinflammation is a key mechanism in the progression (e.g., AD and PD) or secondary injury development (e.g., stroke, hemorrhage, stress, and TBI) of multiple brain conditions. Hence, it provides an opportunity for the therapeutic intervention to prevent progressive tissue damage and loss of function. The key for developing anti-neuroinflammatory treatment is to minimize the detrimental and neurotoxic effects of inflammation while promoting the beneficial and neurotropic effects, thereby creating ideal conditions for regeneration and repair. This review outlines how inflammation is involved in the pathogenesis of major nonpathogenic neuroinflammatory conditions and discusses the complex response of glial cells to damage signals. In addition, emerging experimental anti-neuroinflammatory drug treatment strategies are discussed.
Palladium on Layered Double Hydroxide: A Heterogeneous System for the Enol Phosphate Carbon-Oxygen Bond Activation in Aqueous Media
Authors: Senra Jaqueline D. , Silva Aires C. , Santos Raquel V. , Malta Luiz Fernando B. , Simas Alessandro B. C.
Published: January 2017

In this work, a new catalytic approach for the C-O activation of enol phosphates based on a palladium supported on layered double hydroxide was developed. In this case, two different ketene aminal phosphates were used as models to study the synthesis ofα-phenyl enecarbamates N-Boc/CBz under the Suzuki-Miyaura conditions. The use of an ortho-bromoaniline as precursor allowed the synthesis of the 2-phenyl indole through an arylation/Heck cyclization. Catalyst reusability enabled the synthesis of the heterocycle in moderate yields for four consecutive runs.
Online Estimation of the Adhesion Coefficient and Its Derivative Based on the Cascading SMC Observer
Authors: Zhang Changfan , Sun Jian , He Jing , Liu Linfan
Published: January 2017

The adhesion coefficient of wheel-rail surface cannot be directly measured, so a cascaded sliding-mode observer is proposed to observe adhesion coefficient and its derivative. A kinetic model for running heavy-duty locomotive is also established. The state equation of wheel adhesion control system is derived from the equation of traction motor torque balance, and adhesion coefficient is proposed to be calculated by load torque. Then, the cascaded sliding-mode observer is designed, and its stability is justified by Lyapunov stability. Based on the equivalence control principle for sliding-mode variable structure, an algorithm to estimate adhesion coefficient and its derivative is established. The simulation and experimental results are used to verify the effectiveness of the observer with load variations or wheel-rail status changes.
Nutrition and IBD: Malnutrition and/or Sarcopenia? A Practical Guide
Authors: Scaldaferri F. , Pizzoferrato M. , Lopetuso L. R. , Musca T. , Ingravalle F. , Sicignano L. L. , Mentella M. , Miggiano G. , Mele M. C. , Gaetani E. , Graziani C. , Petito V. , Cammarota G. , Marzetti E. , Martone A. , Landi F. , Gasbarrini A.
Published: January 2017

Malnutrition is a major complication of inflammatory bowel disease (IBD). This mini review is focusing on main determinants of malnutrition in IBD, the most important components of malnutrition, including lean mass loss and sarcopenia, as an emerging problem. Each one of these components needs to be well considered in a correct nutritional evaluation of an IBD patient in order to build a correct multidisciplinary approach. The review is then focusing on possible instrumental and clinical armamentarium for the nutritional evaluation.
Characterization of Rain Specific Attenuation and Frequency Scaling Method for Satellite Communication in South Korea
Authors: Shrestha Sujan , Choi Dong-You
Published: January 2017

The attenuation induced by rain is prominent in the satellite communication at Ku and Ka bands. The paper studied the empirical determination of the power law coefficients which support the calculation of specific attenuation from the knowledge of rain rate at Ku and Ka band for Koreasat 6 and COMS1 in South Korea that are based on the three years of measurement. Rain rate data was measured through OTT Parsivel which shows the rain rate of about 50 mm/hr and attenuation of 10.7, 11.6, and 11.3 dB for 12.25, 19.8, and 20.73 GHz, respectively, for 0.01% of the time for the combined values of rain rate and rain attenuation statistics. Comparing with the measured data illustrates the suitability for estimation of signal attenuation in Ku and Ka band whose validation is done through the comparison with prominent rain attenuation models, namely, ITU-R P.618-12 and ITU-R P. 838-3 with the use of empirically determined coefficient sets. The result indicates the significance of the ITU-R recommended regression coefficients of rain specific attenuation. Furthermore, the overview of predicted year-wise rain attenuation estimation for Ka band in the same link as well as different link is studied which is obtained from the ITU-R P. 618-12 frequency scaling method.
Mercury and Organic Matter Concentrations in Lake and Stream Sediments in relation to One Another and to Atmospheric Mercury Deposition and Climate Variations across Canada
Authors: Nasr Mina , Arp Paul A.
Published: January 2017

This article focuses on analyzing the Geological Survey of Canada (GSC) data for total mercury concentrations (THg) in lake and stream sediments. The objective was to quantify how sediment THg varies by (i) sediment organic matter, determined by loss on ignition (LOI) at 500∘C, (ii) atmospheric Hg deposition (atm.Hgdep) as derived from the Global/Regional Atmospheric Heavy Metals Model GRAHM2005, and (iii) mean annual precipitation and mean monthly July and January temperatures (TJuly,TJan). Through regression analyses and averaging by National Topographic System tiles (NTS, 1:250,000 scale), it was found that 40, 70, and 80% of the sediment THg, LOI, and atm.Hgdepvariations were, respectively, related to precipitation,TJuly, andTJan. In detail, lake sediment THg was related to atm.Hgdepand precipitation, while stream sediment THg was related to sediment LOI andTJuly. Plotting sediment THg versus sediment LOI revealed a curvilinear pattern, with highest Hg concentrations at intermediate LOI values. Analysing the resulting 10th and 90th log10THg percentiles within each 10% LOI class from 0 to 100% revealed that (i) atm.Hgdepcontributed to the organic component of sediment THg and (ii) this was more pronounced for lakes than for streams.
The Production of Neutral N∗(11052) Resonance with Hidden Beauty from π-p Scattering
Authors: Cheng Chen , Wang Xiao-Yun
Published: January 2017

We investigate the discovery potential of the predicted neutral hidden beauty N∗(11052) resonance through π-p scattering within an effective Lagrangian approach. Two reactions π-p→K-Σ+ and π-p→ηbn are studied in this work, with nucleon pole exchange as the background. It is found that the contributions of the N∗(11052) resonance give clear peak structures in the magnitude of 1 μb near the threshold of N∗(11052) in the total cross sections. The numerical results indicate that the center of mass energy W≃11–11.1 GeV would be the best energy window for searching the N∗(11052) resonance, where the N∗(11052) signal can be easily distinguished from the background. The COMPASS experiment at CERN’s Super Proton Synchrotron (SPS) with pion beam of ≃280 GeV will be an ideal platform for searching the super-heavy resonance with hidden beauty, which is promising for testing the theoretical results.
Bufalin Inhibits the Inflammatory Effects in Asthmatic Mice through the Suppression of Nuclear Factor-Kappa B Activity
Authors: Zhakeer Zibierguli , Hadeer Maierbati , Tuerxun Zumulaiti , Tuerxun Kelibiena
Published: January 2017

Asthma is an inflammatory airway disease characterized by increased infiltration of inflammatory cells into the airways and poor respiratory function. Bufalin is one of the biological ingredients obtained from Chansu. Bufalin was found to possess various pharmacological properties including anti-inflammatory activities. However, the effect of bufalin treatment on asthma has not yet been reported. Therefore, this study aimed to investigate the inhibitory effect of bufalin on asthmatic response in a murine model. A mouse asthma model was developed by ovalbumin (OVA) sensitization and challenge in the BALB/c mice. OVA-specific serum IgE and the levels of interleukin (IL)-4, IL-5, and IL-13 in bronchoalveolar lavage fluid (BALF) were determined by an enzyme-linked immunosorbent assay. Recruitment of inflammatory cells into BALF or lung tissues, and goblet cell hyperplasia were evaluated by histological staining. The expression levels of inhibitory subunit of nuclear factor-kappa B (NF-κB) alpha (IκBα) and phosphorylated p65 protein were measured by Western blot analyses. The results demonstrated that bufalin (5 and 10 mg/kg) markedly attenuated hyperresponsiveness, and strongly suppressed the OVA-induced increases of total inflammatory cells including macrophages, eosinophils, lymphocytes, and neutrophils in BALF. The levels of IL-4, IL-5, and IL-13 in BALF and OVA-specific IgE in serum were significantly reduced by bufalin. Histological staining of lung tissues showed that bufalin reduced inflammatory cell infiltration and goblet cell hyperplasia. The results of Western blotting indicated that bufalin suppressed the IκBα degradation from NF-κB, and reduced the level of phosphorylated p65 protein in the lung tissues. These data suggest that bufalin can exert its anti-inflammatory effects possibly through the inhibition of the NF-κB activity.
A Mouse Model of Post-Stroke Pneumonia Induced by Intra-Tracheal Inoculation with Streptococcus pneumoniae
Authors: Mracsko Eva , Stegemann-Koniszewski Sabine , Na Shin-Young , Dalpke Alexander , Bruder Dunja , Lasitschka Felix , Veltkamp Roland
Published: January 2017

Background: Stroke-induced immunodeficiency increases the risk of infectious complications, which adversely affects neurological outcome. Among those, pneumonia affects as many as one third of stroke patients and is the main contributor to mortality in the post-acute phase of stroke. Experimental findings on post-stroke susceptibility to spontaneous pneumonia in mice are contradictory. Here, we established a mouse model inducing standardized bacterial pneumonia and characterized the impaired pulmonary cellular and humoral immune responses after experimental stroke. Methods: Bacterial pneumonia was induced by intra-tracheal inoculation with Streptococcus pneumoniae at different time points after transient middle cerebral artery occlusion (MCAO). Bacterial counts in lungs and blood, histological changes, and cytokine production in the lungs were assessed. Furthermore, we investigated the effect of pneumonia on stroke outcome. Results: Intra-tracheal inoculation resulted in reproducible pneumonia and bacteraemia, and demonstrated post-stroke susceptibility to streptococcal pneumonia developing with a delay of at least 24 h after MCAO. Higher bacterial counts in mice infected 3 days after stroke induction correlated with reduced neutrophil and macrophage infiltration in the lungs and lower levels of pro-inflammatory cytokines in the broncho-alveolar lavage compared to sham-operated animals. Pneumonia increased mortality without affecting brain-infiltrating leukocytes. Conclusions: In this standardized mouse model of post-stroke pneumonia, we describe attenuated leukocyte infiltration and cytokine production in response to bacterial infection in the lungs that has a profound effect on outcome.
Diagnostic Value of Serum Baseline Tryptase Levels in Childhood Asthma and Its Correlation with Disease Severity
Authors: Gao Siju , Fan Jingang , Wang Zhaoan
Published: January 2017

Background: The aim of this study was to explore whether serum baseline tryptase (sBT) levels might be a useful marker not only for the accurate diagnosis of childhood asthma, but also for the prediction of disease severity. Methods: A total of 114 asthmatic children were enrolled in this study, 36 of whom had mild intermittent asthma, 38 had mild persistent asthma, and 40 had moderate to severe persistent asthma. Additionally, 34 age-matched healthy children were enrolled as controls. The sBT levels of these populations were measured using a fluoroenzymeimmunoassay kit. The diagnostic performance of sBT levels and their correlation with asthma severity were systematically investigated using receiver operating characteristic (ROC) analysis and correlation analysis. Results: Children with mild and moderate to severe persistent asthma had significantly increased sBT levels as compared to those with mild intermittent asthma and healthy controls. ROC analysis further demonstrated that sBT levels not only appear to be highly sensitive and specific for distinguishing asthmatic children from healthy controls, but also show good accuracy for the differentiation of various asthmatic subgroups. Correlation analysis revealed that in all asthmatic subgroups sBT levels were significantly correlated with a variety of key markers that reflect the disease severity of asthma, including childhood asthma control test scores, serum IgE and interleukin-13 levels, blood eosinophil counts, and pulmonary test parameters. Conclusions: sBT levels may have a potential use in supporting a diagnosis of asthma in children and as a predictor of disease severity.
The Involvement of Protein Kinase D in T Cell-Induced Mast Cell Activation
Authors: Salamon Pazit , Shefler Irit , Hershko Alon Y. , Mekori Yoseph A.
Published: January 2017

Background: It has recently been reported that mast cells (MC) can be activated to degranulate and release certain cytokines in response to direct physical contact with activated but not resting T cells or their membranes. The MAPK family members ERK and p38 were found to participate. In this work, we further characterize the signaling events involved in this novel pathway of activation. Methods: Human MC were stimulated by activated T cell membranes (T*m). Phosphorylation of kinases was assessed by Western blotting. Protein kinase D (PKD) translocation was visualized by confocal microscopy. Degranulation was assessed by β-hexosaminidase release and cytokine production by ELISA. Results: Stimulation of human MC by activated T*m resulted in the activation of PKD. PKD inhibition by the specific pharmacological inhibitor Gö6976 resulted in a reduction in the phosphorylation of p38 but not ERK. Gö6976 also inhibited degranulation and cytokine release. Conclusions: MC stimulation by physical contact with T cells results in PKD activation, leading to the phosphorylation of p38, degranulation and release of cytokines. Understanding the molecular events associated with T cell-induced MC activation might lead to therapeutic approaches for controlling T cell-mediated inflammatory processes in which MC participate.
Mechanism of 2,3,4',5-Tetrahydroxystilbene 2-O-β-D-Glucoside-Induced Upregulation of Glutamate Transporter 1 Protein Expression in Mouse Primary Astrocytes
Authors: Chen Xiangfan , Hu Wenfeng , Lu Xu , Jiang Bo , Wang Jili , Zhang Wei , Huang Chao
Published: January 2017

Glutamate transporter-1 (GLT-1), a major glutamate transporter expressed in astrocytes, takes up excess glutamate from the micro-environment in order to prevent excitotoxicity. Drugs that increase GLT-1 expression may have therapeutic effects in disorders associated with neuronal excitotoxicity. 2,3,4',5-tetrahydroxystilbene 2-O-β-D-glucoside (TSG), a monomer of stilbene from polygonummultiflorum, exerts neuroprotection in a range of experimental models such as Alzheimer's disease and brain ischemia. In this study, we evaluated the effect of TSG on GLT-1 protein expression in mouse primary-cultured astrocytes. Results showed that TSG markedly increased the GLT-1 protein expression level in mouse primary-cultured astrocytes in a dose- and time-dependent manner, and this increase was mediated by the activation of protein kinase B (Akt) but not by the activation of extracellular signal-regulated protein kinase 1/2. Furthermore, inhibition of cAMP response element-binding protein, but not nuclear factor kappa B, abolished the TSG-mediated increase in GLT-1 protein expression in cultured astrocytes. Collectively, these findings may provide novel insights into the mechanism for TSG in neuroprotection, and would help search new agents targeting neurodegenerative disorders associated with impaired astrocytic glutamate transporters.
Refining Prognosis for Intracerebral Hemorrhage by Early Reassessment
Authors: Maas Matthew B. , Francis Brandon A. , Sangha Rajbeer S. , Lizza Bryan D. , Liotta Eric M. , Naidech Andrew M.
Published: January 2017

Background: Prognostic assessments, which are crucial for decision-making in critical illnesses, have shown unsatisfactory reliability. We compared the accuracy of a widely used prognostic score against a model derived from clinical data obtained 5 days after admission for patients with intracerebral hemorrhage (ICH), a condition for which prognostication has proven notoriously challenging and prone to bias. Methods: Patients enrolled in a prospective observational cohort study of spontaneous ICH underwent hourly Glasgow Coma Scale (GCS) assessment. Outcome was measured at 3 months using the modified Rankin Scale (mRS). We analyzed the change in correlation between GCS and 3-month mRS scores from admission through day 5, and compared the performance of a parsimonious set of day 5 clinical variables against the ICH score. Results: Data was collected on 254 subjects. The ICH score and day 5 GCS score were both correlated with 3-month mRS score (p < 0.001), but the correlation was stronger with day 5 GCS score (p < 0.05 by Fisher z-transformation). Premorbid mRS score, intraventricular hemorrhage and day 5 GCS score were independent predictors of outcome (all p < 0.05 in ordinal regression model). While ICH score correctly classified good (mRS 0-3) vs. poor (mRS 4-6) outcome in 73% of cases, the day 5 model correctly classified 83% of cases. Conclusions: A simple reassessment after 5 days of care significantly improves the accuracy of prognosticating outcome in patients with ICH. These data confirm the feasibility and potential utility of early reassessments in refining prognosis for patients who survive early stabilization of a severe neurologic injury.
Combined Memantine and Donepezil Treatment Improves Behavioral and Psychological Symptoms of Dementia-Like Behaviors in Olfactory Bulbectomized Mice
Authors: Yabuki Yasushi , Matsuo Kazuya , Hirano Koga , Shinoda Yasuharu , Moriguchi Shigeki , Fukunaga Kohji
Published: January 2017

Memantine, an uncompetitive N-methyl-D-aspartate receptor antagonist, and the cholinesterase inhibitor, donepezil, are approved in most countries for treating moderate-to-severe Alzheimer's disease (AD). These drugs have different molecular targets; thus, it is expected that the effects of combined treatment would be synergistic. Some reports do show memantine/donepezil synergy in ameliorating cognition in AD model animals, but their combined effects on behavioral and psychological symptoms of dementia (BPSD)-like behaviors have not been addressed. Here, we investigate combined memantine/donepezil effects on cognitive impairment and BPSD-like behaviors in olfactory bulbectomized (OBX) mice. Interestingly, combined administration synergistically improved both depressive-like behaviors and impaired social interaction in OBX mice, whereas only weak synergistic effects on cognitive performance were seen. To address mechanisms underlying these effects, we used in vivo microdialysis study and observed impaired nicotine-induced serotonin (5-HT) release in OBX mouse hippocampus. Combined memantine/donepezil administration, but not single administration of either, significantly antagonized the decrease in nicotine-induced 5-HT release seen in OBX mouse hippocampus. Furthermore, decreased autophosphorylation of calcium/calmodulin dependent protein kinase II (CaMKII) was rescued in hippocampal CA1 and dentate gyrus of OBX mice by combined memantine/donepezil administration. These results suggest that improvement of BPSD-like behaviors by the co-administration of both drugs is in part mediated by enhanced 5-HT release and CaMKII activity in OBX mouse hippocampus.
Diesel Exhaust Particles Enhance MUC4 Expression in NCI-H292 Cells and Nasal Epithelial Cells via the p38/CREB Pathway
Authors: Park Il-Ho , Kang Ju-Hyung , Kim Jin Ah , Shin Jae-Min , Lee Heung-Man
Published: January 2017

Background: Diesel exhaust particles (DEPs), the major contributors to air pollution, induce inflammatory responses in the nasal epithelium. Overproduction of airway mucins is an important pathogenic finding in inflammatory airway diseases. Objective: The aims of the present study were to determine the effect of DEPs on the expression of the mucin gene MUC4 and to investigate the underlying mechanism of DEP-induced MUC4 expression in NCI-H292 cells and primary nasal epithelial cells (PNECs). Methods: NCI-H292 cells were stimulated for 24 h with DEPs. Messenger RNA (mRNA) and protein expression of MUC4 was determined by real-time reverse transcription (RT) polymerase chain reaction (PCR) and Western blotting. NCI-H292 cells were exposed to 3 mitogen-activated protein kinase inhibitors (U0126, SB203580, and SP600125) and a CREB (cAMP response element-binding protein) inhibitor prior to stimulation with DEPs, and MUC4 expression was examined by RT-PCR and Western blotting. PNECs were pretreated with a p38 inhibitor and CREB inhibitor prior to stimulation with DEPs, and MUC4 expression was then determined by RT-PCR and/or Western blotting. Results: DEPs significantly increased the expression of MUC4 mRNA and protein. MUC4 mRNA and protein expression was inhibited by pretreatment with p38 and CREB inhibitors in NCI-H292 stimulated with DEPs. p38 and CREB inhibitors also blocked the expression of MUC4 mRNA and protein in DEP-stimulated PNECs. Conclusions: We demonstrated that DEPs stimulated the expression of MUC4 via the p38/CREB pathway in NCI-H292 cells and PNECs. The results of the present study pave the way for further studies on the role of MUC4 in DEP-induced hypersecretion in airway epithelium.
Association of Polysensitization, Allergic Multimorbidity, and Allergy Severity: A Cross-Sectional Study of School Children
Authors: Ha Eun Kyo , Baek Ji Hyeon , Lee So-Yeon , Park Yong Mean , Kim Woo Kyung , Sheen Youn Ho , Lee Seung Jin , Bae Youngoh , Kim Jihyeon , Lee Kee-Jae , Ahn Kangmo , Kwon Ho-Jang , Han Man Yong
Published: January 2017

Background: Aeroallergen sensitization is related to the coexistence of allergic diseases, but the nature of this relationship is poorly understood. The aim of this study was to clarify the relationship of polysensitization with allergic multimorbidities and the severity of allergic diseases. Methods: This study is a cross-sectional analysis of 3,368 Korean children aged 6-7 years-old. We defined IgE-mediated allergic diseases based on structured questionnaires, and classified the sensitivity to 18 aeroallergens by logistic regression and the Ward hierarchical clustering method. The relationship of polysensitization (positive IgE responses against 2 or more aeroallergens classes) with allergic multimorbidities (coexistence of 2 or more of the following allergic diseases: asthma, rhinitis, eczema, and conjunctivitis) and severity of allergic diseases was determined by ordinal logistic regression analysis. Results: The rate of polysensitization was 13.6% (n = 458, 95% CI 12.4-14.8) and that of allergic multimorbidity was 23.5% (n = 790, 95% CI 22.0-24.9). Children sensitized to more aeroallergens tended to have more allergic diseases (rho = 0.248, p < 0.001), although the agreement between polysensitization and multimorbidity was poor (kappa = 0.11, p < 0.001). The number allergen classes to which a child was sensitized increased the risk of wheezing attacks (1 allergen: adjusted odds ratio [aOR] 2.22, 4 or more allergens: aOR 9.39), absence from school (1 allergen: aOR 1.96, 3 allergens: aOR 2.08), and severity of nasal symptoms (1 allergen: aOR 1.61, 4 or more allergens: aOR 4.38). Conclusion: Polysensitization was weakly related to multimorbidity. However, the number of allergens to which a child is sensitized is related to the severity of IgE-mediated symptoms.
Novel Mechanisms Underlying the Therapeutic Effect of Glycomacropeptide on Allergy: Change in Gut Microbiota, Upregulation of TGF-β, and Inhibition of Mast Cells
Authors: Jiménez Mariela , Cervantes-García Daniel , Muñoz Yualli Haydee , García Alejandra , Haro Jr. Luis Miguel , Salinas Eva
Published: January 2017

Background: The prevalence of allergic diseases is globally increasing. We have previously described that glycomacropeptide (GMP), a bioactive milk peptide, has therapeutic value in experimental models of skin hypersensitivity, anaphylaxis, and asthma, as it prevents an excessive T helper type 2 cell immune response. The aim of this study was to analyze the effect of GMP on key elements directly involved in the development or control of allergy, in order to improve the precise knowledge about its mechanism of action. Methods: Rats were systemically sensitized with ovalbumin and orally treated with GMP. Levels of Lactobacillus, Bifidobacterium, and Bacteroides were analyzed in their feces. Splenocytes were isolated and the production of transforming growth factor (TGF)-β by allergens was measured. Intradermal skin reactions were developed to evaluate in vivo activation of mast cells. Peritoneal mast cells were isolated and activated by the allergen, and histamine secretion was determined. Results: GMP administration increased the amount of intestinal Lactobacillus and Bifidobacterium of allergen-sensitized animals after 3 days of treatment. The increase in Bacteroides was also significant, but only after 17 days of GMP administration. Ten days after treatment cessation, Lactobacillus and Bacteroides were still elevated. GMP intake also elevated the production of TGF-β in the splenocytes of sensitized animals. In addition, treatment with GMP attenuated mast cell activation by the allergen and inhibited histamine secretion, without affecting the number of mast cells. Conclusions: The prebiotic action of GMP on allergy-protective microbiota, an increase in TGF-β production, and a reduction in mast cell response to allergens are novel mechanisms that explain the antiallergic activity of GMP.