bluuebunny/crossref_metadata_2025 huggingface dataset:
The application of distributed optical strain sensing to measure the strain distribution of ground support members
Authors: Forbes Bradley , Vlachopoulos Nicholas , Hyett Andrew J.
Published: February 2018

A distributed optical strain-sensing technique is presented as a solution for measuring the strain distribution along ground support members used in tunnelling and mining works. The technique employs a Rayleigh optical frequency domain reflectometry technology, which measures strain at a spatial resolution of 0.65 mm along the length of a standard optical fiber. A rationale for selecting this technology as a potential monitoring technique for ground support elements over alternative commercially available technologies is discussed. The development of a technique to couple optical fiber sensors with rock bolt, umbrella arch, and cable bolt support members is also demonstrated. A robust laboratory investigation of such optically instrumented support members demonstrated the capability of the technique to capture the expected in situ support behaviour in the form of coaxial, lateral, and shear loading arrangements as would be anticipated in the field. Moreover, the micro-scale data obtained by this optical sensing technique are shown to provide unprecedented insight into the local/micro-scale geomechanistic complexities associated with the bearing capacity of ground support members, especially when compared with data obtained by discrete strain-sensing technologies.
Status of rates and rate equations for thermal leptogenesis
Authors: Biondini S. , Bödeker D. , Brambilla N. , Garny M. , Ghiglieri J. , Hohenegger A. , Laine M. , Mendizabal S. , Millington P. , Salvio A. , Vairo A.
Published: February 2018

In many realizations of leptogenesis, heavy right-handed neutrinos play the main role in the generation of an imbalance between matter and antimatter in the early Universe. Hence, it is relevant to address quantitatively their dynamics in a hot and dense environment by taking into account the various thermal aspects of the problem at hand. The strong washout regime offers an interesting framework to carry out calculations systematically and reduce theoretical uncertainties. Indeed, any matter–antimatter asymmetry generated when the temperature of the hot plasma [Formula: see text] exceeds the right-handed neutrino mass scale [Formula: see text] is efficiently erased, and one can focus on the temperature window [Formula: see text]. We review recent progress in the thermal field theoretic derivation of the key ingredients for the leptogenesis mechanism: the right-handed neutrino production rate, the CP asymmetry in the heavy-neutrino decays and the washout rates. The derivation of evolution equations for the heavy-neutrino and lepton-asymmetry number densities, their rigorous formulation and applicability are also discussed.
Reflectivity of 1D photonic crystals: A comparison of computational schemes with experimental results
Authors: Pérez-Huerta J. S. , Ariza-Flores D. , Castro-García R. , Mochán W. L. , Ortiz G. P. , Agarwal V.
Published: February 2018

We report the reflectivity of one-dimensional finite and semi-infinite photonic crystals, computed through the coupling to Bloch modes (BM) and through a transfer matrix method (TMM), and their comparison to the experimental spectral line shapes of porous silicon (PS) multilayer structures. Both methods reproduce a forbidden photonic bandgap (PBG), but slowly-converging oscillations are observed in the TMM as the number of layers increases to infinity, while a smooth converged behavior is presented with BM. The experimental reflectivity spectra is in good agreement with the TMM results for multilayer structures with a small number of periods. However, for structures with large amount of periods, the measured spectral line shapes exhibit better agreement with the smooth behavior predicted by BM.
FRACTAL NATURE OF POROSITY IN VOLCANIC TIGHT RESERVOIRS OF THE SANTANGHU BASIN AND ITS RELATIONSHIP TO PORE FORMATION PROCESSES
Authors: WANG WEIMING , WANG ZHIXUAN , CHEN XUAN , LONG FEI , LU SHUANGFANG , LIU GUOHONG , TIAN WEICHAO , SU YUE
Published: February 2018

In this paper, in a case study of Santanghu Basin in China, the morphological characteristics and size distribution of nanoscale pores in the volcanic rocks of the Haerjiawu Formation were investigated using the results of low temperature nitrogen adsorption experiments. This research showed that within the target layer, a large number of nanoscale, eroded pores showed an “ink bottle” morphology with narrow pore mouths and wide bodies. The fractal dimension of pores increases gradually with increasing depth. Moreover, as fractal dimension increases, BET-specific surface area gradually increases, average pore diameter decreases and total pore volume gradually increases. The deeper burial of the Haerjiawu volcanic rocks in the Santanghu Basin leads to more intense erosion by organic acids derived from the basin’s source rocks. Furthermore, the internal surface roughness of these corrosion pores results in poor connectivity. As stated above, the corrosion process is directly related to the organic acids generated by the source rock of the interbedded volcanic rocks. The deeper the reservoir, the more the organic acids being released from the source rock. However, due to the fact that the Haerjiawu volcanic rocks are tight reservoirs and have complicated pore-throat systems, while organic acids dissolve unstable minerals such as feldspars which improve the effective reservoir space; the dissolution of feldspars results in the formation of new minerals, which cannot be expelled from the tight reservoirs. They are instead precipitated in the fine pore throats, thereby reducing pore connectivity, while enhancing reservoir micro-preservation conditions.
Energy Labeling System of Urban Residential Buildings: Market Effect and Operating Mechanism — A Case Study of Energy-Saving Renovations in the Netherlands and Its Implications
Authors: JIANG Wei , ZHANG Xuhui
Published: February 2018

Despite China's significant progress in energy saving renovations, during the past 10 years, problems about inefficiencies remain. In the Netherlands, the energy labeling system (ELS) effectively linked policy objectives and market forces, combined with the stepped tariffs aimed at the performance of energy-saving renovation, generating a virtuous cycle of housing energy efficiency upgrading. China may draw the experience from Netherlands. In this regard, the authors probe the market effect and operating mechanism of the Dutch ELS and the stepped tariffs. The theory of multi-level governance (MLG) is introduced to the filed investigations both in China and the Netherlands. Based on the group-interviews and depth-interviews with the officials in related agencies and the residents of retrofitting housing, the authors obtained first-hand information to ensure a close case study on Netherlands' housing ELS and its implementation, in order to provide some enlightenment for China's existing housing renovation and low carbon development.
Solar Photochemical Reduction and Oxidation of Water and Related Aspects
Authors: Lingampalli S.R. , Rao C.N.R.
Published: February 2018

Conversion of solar energy to useful chemicals has become necessary for finding solutions to energy and environmental issues. One of the means is to use of solar energy for the reduction of water to generate hydrogen or for the reduction of CO[Formula: see text] to useful chemicals. In spite of substantial effort, the discovery of stable and efficient photocatalysts remains a challenge, although some encouraging results have been reported. In this article, we provide a brief perspective of the current status of solar water splitting and reduction of CO[Formula: see text].
Genomics-Informed Insights into Endosymbiotic Organelle Evolution in Photosynthetic Eukaryotes
Authors: Nowack Eva C.M. , Weber Andreas P.M.
Published: February 2018

The conversion of free-living cyanobacteria to photosynthetic organelles of eukaryotic cells through endosymbiosis transformed the biosphere and eventually provided the basis for life on land. Despite the presumable advantage conferred by the acquisition of photoautotrophy through endosymbiosis, only two independent cases of primary endosymbiosis have been documented: one that gave rise to the Archaeplastida, and the other to photosynthetic species of the thecate, filose amoeba Paulinella. Here, we review recent genomics-informed insights into the primary endosymbiotic origins of cyanobacteria-derived organelles. Furthermore, we discuss the preconditions for the evolution of nitrogen-fixing organelles. Recent genomic data on previously undersampled cyanobacterial and protist taxa provide new clues to the origins of the host cell and endosymbiont, and proteomic approaches allow insights into the rearrangement of the endosymbiont proteome during organellogenesis. We conclude that in addition to endosymbiotic gene transfers, horizontal gene acquisitions from a broad variety of prokaryotic taxa were crucial to organelle evolution.
Exploring the Spatiotemporal Organization of Membrane Proteins in Living Plant Cells
Authors: Wang Li , Xue Yiqun , Xing Jingjing , Song Kai , Lin Jinxing
Published: February 2018

Plasma membrane proteins have important roles in transport and signal transduction. Deciphering the spatiotemporal organization of these proteins provides crucial information for elucidating the links between the behaviors of different molecules. However, monitoring membrane proteins without disrupting their membrane environment remains difficult. Over the past decade, many studies have developed single-molecule techniques, opening avenues for probing the stoichiometry and interactions of membrane proteins in their native environment by providing nanometer-scale spatial information and nanosecond-scale temporal information. In this review, we assess recent progress in the development of labeling and imaging technology for membrane protein analysis. We focus in particular on several single-molecule techniques for quantifying the dynamics and assembly of membrane proteins. Finally, we provide examples of how these new techniques are advancing our understanding of the complex biological functions of membrane proteins.
Reactive Oxygen Species in Plant Signaling
Authors: Waszczak Cezary , Carmody Melanie , Kangasjärvi Jaakko
Published: February 2018

As fixed organisms, plants are especially affected by changes in their environment and have consequently evolved extensive mechanisms for acclimation and adaptation. Initially considered by-products from aerobic metabolism, reactive oxygen species (ROS) have emerged as major regulatory molecules in plants and their roles in early signaling events initiated by cellular metabolic perturbation and environmental stimuli are now established. Here, we review recent advances in ROS signaling. Compartment-specific and cross-compartmental signaling pathways initiated by the presence of ROS are discussed. Special attention is dedicated to established and hypothetical ROS-sensing events. The roles of ROS in long-distance signaling, immune responses, and plant development are evaluated. Finally, we outline the most challenging contemporary questions in the field of plant ROS biology and the need to further elucidate mechanisms allowing sensing, signaling specificity, and coordination of multiple signals.
Meiotic Recombination: Mixing It Up in Plants
Authors: Wang Yingxiang , Copenhaver Gregory P.
Published: February 2018

Meiosis halves diploid chromosome numbers to haploid levels that are essential for sexual reproduction in most eukaryotes. Meiotic recombination ensures the formation of bivalents between homologous chromosomes (homologs) and their subsequent proper segregation. It also results in genetic diversity among progeny that influences evolutionary responses to selection. Moreover, crop breeding depends upon the action of meiotic recombination to rearrange elite traits between parental chromosomes. An understanding of the molecular mechanisms that drive meiotic recombination is important for both fundamental research and practical applications. This review emphasizes advances made during the past 5 years, primarily in Arabidopsis and rice, by summarizing newly characterized genes and proteins and examining the regulatory mechanisms that modulate their action.
Enhancing Analytical Separations Using Super-Resolution Microscopy
Authors: Moringo Nicholas A. , Shen Hao , Bishop Logan D.C. , Wang Wenxiao , Landes Christy F.
Published: February 2018

Super-resolution microscopy is becoming an invaluable tool to investigate structure and dynamics driving protein interactions at interfaces. In this review, we highlight the applications of super-resolution microscopy for quantifying the physics and chemistry that occur between target proteins and stationary-phase supports during chromatographic separations. Our discussion concentrates on the newfound ability of super-resolved single-protein spectroscopy to inform theoretical parameters via quantification of adsorption-desorption dynamics, protein unfolding, and nanoconfined transport.
Redistribution Without a Median Voter: Models of Multidimensional Politics
Authors: Iversen Torben , Goplerud Max
Published: February 2018

Most work on redistribution in democracies is anchored in long-standing unidimensional models, notably the seminal Meltzer-Richard-Romer model. When scholars venture outside the security of unidimensionality, many either abandon theoretical rigor or miss the full consequences of adding more dimensions (whether ideological or economic). There is now a substantial literature on redistributive politics in multidimensional policy spaces, but it tends to be very technical and frequently misinterpreted, if not ignored. This purpose of this article is to review this relatively new literature using simple graphical representations, focusing on the key assumptions, intuitions, and results. We show how issue bundling, issue salience, and the distribution of preferences can affect redistribution, and we discuss the role of political institutions in inducing particular outcomes. We also highlight the opportunities for dialogue between formal models and more constructivist approaches by exploring the effects of political entrepreneurs manipulating salience, institutions, and even identities.
Mitochondrial inner membrane protein (mitofilin) knockdown induces cell death by apoptosis via an AIF-PARP-dependent mechanism and cell cycle arrest
Authors: Madungwe Ngonidzashe B. , Feng Yansheng , Lie Mihaela , Tombo Nathalie , Liu Li , Kaya Ferdinand , Bopassa Jean C.
Published: February 2018

Mitofilin is an inner membrane protein that has been defined as a mitochondria-shaping protein in controlling and maintaining mitochondrial cristae structure and remodeling. We determined the role of mitofilin in cell survival by investigating the mechanism underlying mitofilin knockdown-induced cell death by apoptosis. Cultured H9c2 myoblasts and HEK 293 cells were treated with mitofilin siRNA or scrambled siRNA for 24 h. Cell death (apoptosis), caspase 3 activity and cell cycle phases were assessed by flow cytometry, while cytochrome c release and intracellular ATP production were measured by ELISA. Mitofilin, apoptosis-inducing factor (AIF) and poly(ADP-ribose) polymerase (PARP) expression were measured by Western blot analysis and calpain activity was assessed using a calpain activity kit. Mitochondrial images were taken using electron microscopy. We found that mitofilin knockdown increases apoptosis mainly via activation of the AIF-PARP pathway leading to nuclear fragmentation that is correlated with S phase arrest of the cell cycle. Knockdown of mitofilin also led to mitochondrial swelling and damage of cristae that is associated with the increase in reactive oxygen species production and mitochondrial calpain activity, as well as a marked decrease in intracellular ATP production and mitochondrial membrane potential. Together, these results indicate that mitofilin knockdown by siRNA increases calpain activity that presumably leads to mitochondrial structural degradation resulting in a critical reduction of mitochondrial function that is responsible for the increase in cell death by apoptosis via an AIF-PARP mechanism and associated with nuclear fragmentation, and S phase arrest of the cell cycle.
Highs and lows of hyperoxia: physiological, performance, and clinical aspects
Authors: Brugniaux Julien Vincent , Coombs Geoff B. , Barak Otto F. , Dujic Zeljko , Sekhon Mypinder S. , Ainslie Philip N.
Published: February 2018

Molecular oxygen (O2) is a vital element in human survival and plays a major role in a diverse range of biological and physiological processes. Although normobaric hyperoxia can increase arterial oxygen content ([Formula: see text]), it also causes vasoconstriction and hence reduces O2delivery in various vascular beds, including the heart, skeletal muscle, and brain. Thus, a seemingly paradoxical situation exists in which the administration of oxygen may place tissues at increased risk of hypoxic stress. Nevertheless, with various degrees of effectiveness, and not without consequences, supplemental oxygen is used clinically in an attempt to correct tissue hypoxia (e.g., brain ischemia, traumatic brain injury, carbon monoxide poisoning, etc.) and chronic hypoxemia (e.g., severe COPD, etc.) and to help with wound healing, necrosis, or reperfusion injuries (e.g., compromised grafts). Hyperoxia has also been used liberally by athletes in a belief that it offers performance-enhancing benefits; such benefits also extend to hypoxemic patients both at rest and during rehabilitation. This review aims to provide a comprehensive overview of the effects of hyperoxia in humans from the “bench to bedside.” The first section will focus on the basic physiological principles of partial pressure of arterial O2, [Formula: see text], and barometric pressure and how these changes lead to variation in regional O2delivery. This review provides an overview of the evidence for and against the use of hyperoxia as an aid to enhance physical performance. The final section addresses pathophysiological concepts, clinical studies, and implications for therapy. The potential of O2toxicity and future research directions are also considered.
Identification of transcripts associated with renal damage due to ureteral obstruction as candidate urinary biomarkers
Authors: Wu Bo , Gong Xue , Kennedy William A. , Brooks James D.
Published: February 2018

Renal obstruction is a common cause of renal failure in adults and children and is suspected when hydronephrosis is detected on imaging. Because not all cases of hydronephrosis are associated with renal damage, biomarkers are needed to guide intervention to relieve obstruction. We performed gene expression profiling on the kidneys from adult mice over a detailed time course after obstruction and compared these data with a neonatal model of bilateral high-grade obstruction induced by conditional deletion of the calcineurin β1 gene. Having identified a set of 143 transcripts modulated in both adult and neonatal obstruction, we tested their expression in a model of short-term obstruction (1 day), where renal damage is transient and reversible, and long-term obstruction (5 days), where significant renal damage is permanent. A significant number of transcripts increased early after obstruction, and later normalized, while 26 transcripts remained elevated 10 and 28 days after relief of 5 days of ureteral obstruction. With the use of qPCR, elevated levels of several of these candidate RNA biomarkers of renal damage were detected in urine from obstructed mice. In addition, several of these candidate RNA biomarkers of damage resulting from obstruction were detectable in catheterized urine samples from children undergoing surgery for ureteropelvic junction obstruction. Measurement of urinary transcripts modulated in response to renal obstruction could serve as biomarkers of renal damage with important clinical applications.
Computer-aided neurophysiology and imaging with open-source PhysImage
Authors: Hayes John A. , Papagiakoumou Eirini , Ruffault Pierre-Louis , Emiliani Valentina , Fortin Gilles
Published: February 2018

Improved integration between imaging and electrophysiological data has become increasingly critical for rapid interpretation and intervention as approaches have advanced in recent years. Here, we present PhysImage, a fork of the popular public-domain ImageJ that provides a platform for working with these disparate sources of data, and we illustrate its utility using in vitro preparations from murine embryonic and neonatal tissue. PhysImage expands ImageJ’s core features beyond an imaging program by facilitating integration, analyses, and display of 2D waveform data, among other new features. Together, with the Micro-Manager plugin for image acquisition, PhysImage substantially improves on closed-source or blended approaches to analyses and interpretation, and it furthermore aids post hoc automated analysis of physiological data when needed as we demonstrate here. Developing a high-throughput approach to neurophysiological analyses has been a major challenge for neurophysiology as a whole despite data analytics methods advancing rapidly in other areas of neuroscience, biology, and especially genomics.

NEW & NOTEWORTHY High-throughput analyses of both concurrent electrophysiological and imaging recordings has been a major challenge in neurophysiology. We submit an open-source solution that may be able to alleviate, or at least reduce, many of these concerns by providing an institutionally proven mechanism (i.e., ImageJ) with the added benefits of open-source Python scripting of PhysImage data that eases the workmanship of 2D trace data, which includes electrophysiological data. Together, with the ability to autogenerate prototypical figures shows this technology is a noteworthy advance.
Spatiotemporal evolution of focal epileptiform activity from surface and laminar field recordings in cat neocortex
Authors: Bink Hank , Sedigh-Sarvestani Madineh , Fernandez-Lamo Ivan , Kini Lohith , Ung Hoameng , Kuzum Duygu , Vitale Flavia , Litt Brian , Contreras Diego
Published: February 2018

New devices that use targeted electrical stimulation to treat refractory localization-related epilepsy have shown great promise, although it is not well known which targets most effectively prevent the initiation and spread of seizures. To better understand how the brain transitions from healthy to seizing on a local scale, we induced focal epileptiform activity in the visual cortex of five anesthetized cats with local application of the GABAA blocker picrotoxin while simultaneously recording local field potentials on a high-resolution electrocorticography array and laminar depth probes. Epileptiform activity appeared in the form of isolated events, revealing a consistent temporal pattern of ictogenesis across animals with interictal events consistently preceding the appearance of seizures. Based on the number of spikes per event, there was a natural separation between seizures and shorter interictal events. Two distinct spatial regions were seen: an epileptic focus that grew in size as activity progressed, and an inhibitory surround that exhibited a distinct relationship with the focus both on the surface and in the depth of the cortex. Epileptiform activity in the cortical laminae was seen concomitant with activity on the surface. Focus spikes appeared earlier on electrodes deeper in the cortex, suggesting that deep cortical layers may be integral to recruiting healthy tissue into the epileptic network and could be a promising target for interventional devices. Our study may inform more effective therapies to prevent seizure generation and spread in localization-related epilepsies.

NEW & NOTEWORTHY We induced local epileptiform activity and recorded continuous, high-resolution local field potentials from the surface and depth of the visual cortex in anesthetized cats. Our results reveal a consistent pattern of ictogenesis, characterize the spatial spread of the epileptic focus and its relationship with the inhibitory surround, and show that focus activity within events appears earliest in deeper cortical layers. These findings have potential implications for the monitoring and treatment of refractory epilepsy.
Neuronal Cell Death
Authors: Fricker Michael , Tolkovsky Aviva M. , Borutaite Vilmante , Coleman Michael , Brown Guy C.
Published: February 2018

Neuronal cell death occurs extensively during development and pathology, where it is especially important because of the limited capacity of adult neurons to proliferate or be replaced. The concept of cell death used to be simple as there were just two or three types, so we just had to work out which type was involved in our particular pathology and then block it. However, we now know that there are at least a dozen ways for neurons to die, that blocking a particular mechanism of cell death may not prevent the cell from dying, and that non-neuronal cells also contribute to neuronal death. We review here the mechanisms of neuronal death by intrinsic and extrinsic apoptosis, oncosis, necroptosis, parthanatos, ferroptosis, sarmoptosis, autophagic cell death, autosis, autolysis, paraptosis, pyroptosis, phagoptosis, and mitochondrial permeability transition. We next explore the mechanisms of neuronal death during development, and those induced by axotomy, aberrant cell-cycle reentry, glutamate (excitoxicity and oxytosis), loss of connected neurons, aggregated proteins and the unfolded protein response, oxidants, inflammation, and microglia. We then reassess which forms of cell death occur in stroke and Alzheimer’s disease, two of the most important pathologies involving neuronal cell death. We also discuss why it has been so difficult to pinpoint the type of neuronal death involved, if and why the mechanism of neuronal death matters, the molecular overlap and interplay between death subroutines, and the therapeutic implications of these multiple overlapping forms of neuronal death.
Dihydromyricetin Attenuates TNF-α-Induced Endothelial Dysfunction through miR-21-Mediated DDAH1/ADMA/NO Signal Pathway
Authors: Yang Dafeng , Tan Shenglan , Yang Zhousheng , Jiang Pei , Qin Caie , Yuan Qiong , Dang Ruili , Yao Xiaoxia , Qu Jian , Lu Qiong , Xu Ping , Zhang Bikui , Xiang Daxiong , Chen Lei
Published: February 2018

Accumulating studies demonstrate that dihydromyricetin (DMY), a compound extracted from Chinese traditional herb, Ampelopsis grossedentata, attenuates atherosclerotic process by improvement of endothelial dysfunction. However, the underlying mechanism remains poorly understood. Thus, the aim of this study is to investigate the potential mechanism behind the attenuating effects of DMY on tumor necrosis factor alpha- (TNF-α-) induced endothelial dysfunction. In response to TNF-α, microRNA-21 (miR-21) expression was significantly increased in human umbilical vein endothelial cells (HUVECs), in line with impaired endothelial dysfunction as evidenced by decreased tube formation and migration, endothelial nitric oxide synthase (eNOS) (ser1177) phosphorylation, dimethylarginine dimethylaminohydrolases 1 (DDAH1) expression and metabolic activity, and nitric oxide (NO) concentration as well as increased asymmetric dimethylarginine (ADMA) levels. In contrast, DMY or blockade of miR-21 expression ameliorated endothelial dysfunction in HUVECs treated with TNF-α through downregulation of miR-21 expression, whereas these effects were abolished by overexpression of miR-21. In addition, using a nonspecific NOS inhibitor, L-NAME, also abrogated the attenuating effects of DMY on endothelial dysfunction. Taken together, these data demonstrated that miR-21-mediated DDAH1/ADMA/NO signal pathway plays an important role in TNF-α-induced endothelial dysfunction, and DMY attenuated endothelial dysfunction induced by TNF-α in a miR-21-dependent manner.
Effects of Pravastatin on Type 1 Diabetic Rat Heart with or without Blood Glycemic Control
Authors: Min Jeong Jin , Shin Byung-Seop , Lee Jong-Hwan , Jeon Yunseok , Ryu Dae Kyun , Kim Sojin , Shin Young Hee
Published: February 2018

Although statins have been suggested to attenuate the progression of diabetic cardiomyopathy, its effect without glycemic control remains unclear. Therefore, we evaluated the effect of pravastatin on diabetic rat hearts according to glycemic control. Rats were randomly divided into five groups: control (C), diabetes (D), diabetes with insulin (I), diabetes with pravastatin (P), and diabetes with insulin and pravastatin (IP). Eight weeks after allocated treatments, the heart was extracted and analyzed following echocardiography. Cardiac fibrosis was measured using Masson’s trichrome stain. Cardiac expression of collagen I/III, matrix metalloproteinase (MMP)-2, MMP-9, and angiotensin-converting enzyme (ACE)/ACE2 was evaluated by immunohistochemistry and/or Western blot. Enzyme-linked immunosorbent assay was used for measuring reactive oxygen species (ROS). Diabetic groups without glycemic control (D and P) showed significantly impaired diastolic function and increased levels of cardiac fibrosis, collagen I/III, MMP-2, MMP-9, and ROS production. However, there were little significant differences in the outcomes among the control and two glucose-controlled diabetic groups (I and IP). Groups C and IP showed more preserved ACE2 and lower ACE expressions than the other groups did (D, I, and P). Our study suggested glycemic control would be more important to attenuate the progression of diabetic cardiomyopathy than pravastatin medication.
Fatal Pneumococcus Sepsis after Treatment of Late Antibody-Mediated Kidney Graft Rejection
Authors: Einecke Gunilla , Bräsen Jan Hinrich , Hanke Nils , Haller Hermann , Schwarz Anke
Published: February 2018

Antibody-mediated rejection (ABMR) is a major cause of late renal allograft dysfunction and graft loss. Risks and benefits of treatment of late ABMR have not been evaluated in randomized clinical trials. We report on a 35-year-old patient with deterioration in renal function and progressive proteinuria 15 years after transplantation. Recurrent infections after a splenectomy following traumatic splenic rupture 3 years earlier had led to reduction of immunosuppression. Renal transplant biopsy showed glomerular double contours, 40% fibrosis/tubular atrophy, peritubular capillaritis, and positive C4d staining indicating chronic-active ABMR. ABMR treatment was initiated with steroids, plasmapheresis, and rituximab. Fourteen days later, she presented to the emergency department with fever, diarrhea, vomiting, and hypotension. Despite antibiotic treatment she deteriorated with progressive hypotension, capillary leak with pleural effusion, peripheral edema, and progressive respiratory insufficiency. She died due to septic shock five days after admission. Blood cultures showed Streptococcus pneumoniae, consistent with a diagnosis of overwhelming postsplenectomy infection syndrome, despite protective pneumococcus vaccination titers. We assume that the infection was caused by one of the strains not covered by the Pneumovax 23 vaccination. The increased immunosuppression with B cell depletion may have contributed to the overwhelming course of this infection.
Radio and Clutter Suppression Algorithm Based on Spatial Multiple Beams for Small-Aperture HFSWR
Authors: Yao Di , Zhang Xin , Yang Qiang , Deng Weibo
Published: February 2018

Radio and clutter that cover a certain number of range-Doppler-angle cells have a major impact on the detection performance of a high-frequency surface wave radar (HFSWR) system. Especially for a small-aperture array, the angle spectrums of radio and clutter suffer from severe broadening, so the targets that are more easily submerged in the broadened radio and clutter can be hardly detected. To tackle this issue, this paper proposes an algorithm for radio decontamination and clutter suppression to enable detection of the submerged targets. First, the spatial correlation of the array is derived, and the characteristics of radio and clutter are analyzed based on angle-Doppler joint eigenvector. Then owing to the analysis, the information of radio and clutter in the main beam can be accurately estimated from that in the auxiliary beams and eliminated by subtracting it. The results of simulations and measured data indicate that the proposed method offers a significant performance improvement and has a strong robustness against the array amplitude-phase errors.
Equations with Peakon Solutions in the Negative Order Camassa-Holm Hierarchy
Authors: Dong Fengfeng , Zhou Lingjun
Published: February 2018

The negative order Camassa-Holm (CH) hierarchy consists of nonlinear evolution equations associated with the CH spectral problem. In this paper, we show that all the negative order CH equations admit peakon solutions; the Lax pair of theN-order CH equation given by the hierarchy is compatible with its peakon solutions. Special peakon-antipeakon solutions for equations of orders-3and-4are obtained. Indeed, forN≤-2, the peakons ofN-order CH equation can be constructed explicitly by the inverse scattering approach using Stieltjes continued fractions. The properties of peakons forN-order CH equation whenNis odd are much different from the CH peakons; we present the caseN=-3as an example.
Icariin-Loaded TiO2 Nanotubes for Regulation of the Bioactivity of Bone Marrow Cells
Authors: Zhang Yanli , Liu Chundong , Chen Liangjiao , Chen Aijie , Feng Xiaoli , Shao Longquan
Published: February 2018

To explore the effects of icariin on the biocompatibility of dental implants, icariin- (ICA-) loaded TiO2 nanotubes were fabricated on Ti substrates via anodic oxidation and physical absorption. The surface characteristics of the specimens were monitored by field emission scanning electron microscopy (FE-SEM), X-ray diffractometry (XRD), contact angle measurements (CA), and high-pressure liquid chromatography. Additionally, the activities of bone marrow cells, such as cytoskeletal, proliferative activities, mineralization, and osteogenesis-related gene expression on the substrates were investigated in detail. The characterization results demonstrated that ICA-loaded TiO2 nanotubes were successfully fabricated and the hydrophilicity of these TiO2 nanotubes was significantly higher than that of the pure Ti groups. The results also showed that ICA-loaded TiO2 nanotubes might not have enhanced effects on cell proliferation and ALP expression. However, it seemed to significantly promote differentiation of bone marrow cells, demonstrated by enhancing the formation of mineralized nodule and the upregulation of the gene expression such as OC, BSP, OPN, and COL-1. The results indicated that ICA-loaded TiO2 nanotubes can modulate bioactivity of bone marrow cells, which is promising for potential applications in the orthopedics field.
Deciphering Possible Association of Risk Factors for Dental Caries in Pakistani Population
Authors: Maqsood Shahida , Baber Hasan , Abbas Zia , Ali Khan Javeria , Khalid Muznah
Published: February 2018

Obesity is a state of abnormal accumulation of fat in adipose tissues of the body to the level that one’s health is adversely compromised. Tripathi et al. state (according to WHO) that obesity is now considered the fifth leading cause of mortality in the world. Caries is a multifactorial disease and one of the major oral health issues of the modern era affecting people around the globe. Rise in dental caries has been observed in developing countries as a result of factors including increased intake of carbohydrates. The present study aims for assessing the association of DMFT with BMI, age, and gender. This study was conducted in the dental OPD of the Dow University of Health Sciences, Karachi, from February 2016 till January 2017. A custom-made interview-based questionnaire was used to assess BMI, DMFT, and sociodemographics. The sample size was kept at 385. Age was reported as a strong predictor (R2 0.641) of DMFT followed by BMI and gender as the weakest predictors. Age and BMI had statistically significant association with DMFT scores, which shows that diet patterns may affect general health. High caloric intake over long periods affects BMI and also oral health.
Identification of Forensically Important Calliphoridae and Sarcophagidae Species Collected in Korea Using SNaPshot Multiplex System Targeting the Cytochrome c Oxidase Subunit I Gene
Authors: Park Ji Hye , Shin Sang Eon , Ko Kwang Soo , Park Seong Hwan
Published: February 2018

Estimation of postmortem interval (PMI) is paramount in modern forensic investigation. After the disappearance of the early postmortem phenomena conventionally used to estimate PMI, entomologic evidence provides important indicators for PMI estimation. The age of the oldest fly larvae or pupae can be estimated to pinpoint the time of oviposition, which is considered the minimum PMI (PMImin). The development rate of insects is usually temperature dependent and species specific. Therefore, species identification is mandatory forPMIminestimation using entomological evidence. The classical morphological identification method cannot be applied when specimens are damaged or have not yet matured. To overcome this limitation, some investigators employ molecular identification using mitochondrial cytochrome c oxidase subunit I(COI)nucleotide sequences. The molecular identification method commonly uses Sanger’s nucleotide sequencing and molecular phylogeny, which are complex and time consuming and constitute another obstacle for forensic investigators. In this study, instead of using conventional Sanger’s nucleotide sequencing, single-nucleotide polymorphisms (SNPs) in theCOIgene region, which are unique between fly species, were selected and targeted for single-base extension (SBE) technology. These SNPs were genotyped using a SNaPshot® kit. Eleven Calliphoridae and seven Sarcophagidae species were covered. To validate this genotyping, fly DNA samples (103 adults, 84 larvae, and 4 pupae) previously confirmed by DNA barcoding were used. This method worked quickly with minimal DNA, providing a potential alternative to conventional DNA barcoding. Consisting of only a few simple electropherogram peaks, the results were more straightforward compared with those of the conventional DNA barcoding produced by Sanger’s nucleotide sequencing.
Bilateral Hip Arthroplasty: When Is It Safe to Operate the Second Hip? A Systematic Review
Authors: Muskus Meilyn , Rojas Jorge , Gutiérrez Camilo , Guio Juan , Bonilla Guillermo , Llinás Adolfo
Published: February 2018

Introduction. Patients with degenerative hip disease frequently present with bilateral involvement that requires surgical management. The main goal when treating these patients is to achieve the maximum efficiency without increasing risk of perioperative complications; therefore, the decision regarding the best moment to operate the second hip becomes relevant. Although studies have addressed this topic, whether a simultaneous or staged surgery should be performed remains controversial. The purpose of this study was to determine, based on available evidence, the optimum strategy in terms of safety to operate the second hip in patients with bilateral involvement.Materials and Methods. A meta-analysis was planned. A systematic review of the literature was performed including clinical trials or observational analytical studies comparing the safety of bilateral arthroplasty performed simultaneously or staged by measuring major and minor complications. The appropriateness of a meta-analysis was evaluated through the detailed analysis of the risk of bias and clinical heterogeneity of the included studies.Results. Thirteen studies were selected after the systematic review. A wide variability in the methodological designs was found with a critical risk of bias in most of them. Considerable heterogeneity was detected in defining staged surgery in the cointerventions and how the outcomes were defined and measured. In response to these findings, a meta-analysis was considered not appropriate. The results showed no differences in the risk of mortality or systemic complications in young and healthy patients between simultaneous or staged surgeries. However, increased risk of complications for staged surgeries performed during the same hospitalization was observed.Conclusions. Available evidence is very heterogeneous and the quality of evidence is low. The available evidence supports the performance of simultaneous hip arthroplasty in selected patients (not older than 65 years, ASA 1-2, without cardiovascular comorbidities) and suggests the avoidance of staged surgeries within the same hospitalization.
In Vitro Bioactivity of Methanolic Extracts from Amphipterygium adstringens (Schltdl.) Schiede ex Standl., Chenopodium ambrosioides L., Cirsium mexicanum DC., Eryngium carlinae F. Delaroche, and Pithecellobium dulce (Roxb.) Benth. Used in Traditional Medicine in Mexico
Authors: Knauth Peter , Acevedo-Hernández Gustavo J. , Cano M. Eduardo , Gutiérrez-Lomelí Melesio , López Zaira
Published: February 2018

Seven out of eight methanolic extracts from five plants native to Mexico were inactive against ten bacterial strains of clinical interest. The fruit extract of Chenopodium ambrosioides inhibited the bacteria Enterococcus faecalis (MIC = 4375 μg/ml), Escherichia coli (MIC = 1094 μg/ml), and Salmonella typhimurium (MIC = 137 μg/ml). The fruit extract of C. ambrosioides was with CC50 = 45 μg/ml most cytotoxic against the cell‐line Caco‐2, followed by the leaf extract from Pithecellobium dulce (CC50 = 126 μg/ml); interestingly, leaves of C. ambrosioides (CC50 = 563 μg/ml) and bark of P. dulce (CC50 = 347 μg/ml) extracts were much less cytotoxic. We describe for the first time the cytotoxic effect from extracts of the aerial parts and the flowers of Cirsium mexicanum (CC50 = 323 μg/ml and CC50 = 250 μg/ml, resp.). Phytochemical analysis demonstrated for both extracts high tannin and saponin and low flavonoid content, while terpenoids were found in the flowers. For the first time we report a cytotoxicological study on an extract of Eryngium carlinae (CC50 = 356 μg/ml) and likewise the bark extract from Amphipterygium adstringens (CC50 = 342 μg/ml). In conclusion the fruit extract of C. ambrosioides is a potential candidate for further biological studies.
Exploiting Interslice Correlation for MRI Prostate Image Segmentation, from Recursive Neural Networks Aspect
Authors: Zhu Qikui , Du Bo , Turkbey Baris , Choyke Peter , Yan Pingkun
Published: February 2018

Segmentation of the prostate from Magnetic Resonance Imaging (MRI) plays an important role in prostate cancer diagnosis. However, the lack of clear boundary and significant variation of prostate shapes and appearances make the automatic segmentation very challenging. In the past several years, approaches based on deep learning technology have made significant progress on prostate segmentation. However, those approaches mainly paid attention to features and contexts within each single slice of a 3D volume. As a result, this kind of approaches faces many difficulties when segmenting the base and apex of the prostate due to the limited slice boundary information. To tackle this problem, in this paper, we propose a deep neural network with bidirectional convolutional recurrent layers for MRI prostate image segmentation. In addition to utilizing the intraslice contexts and features, the proposed model also treats prostate slices as a data sequence and utilizes the interslice contexts to assist segmentation. The experimental results show that the proposed approach achieved significant segmentation improvement compared to other reported methods.
Mode‐I Metal‐Composite Interface Fracture Testing for Fibre Metal Laminates
Authors: Manikandan Periyasamy , Chai Gin Boay
Published: February 2018

The main contribution of the present paper is the determination of the mode‐I fracture of metal‐composite interface region for fibre metal laminates (FMLs). A hybrid DCB configuration is proposed to investigate the mode‐I fracture between metal‐composite interface using experimental and numerical approaches. A computationally efficient and reliable finite element model was developed to account for the influence of metal plasticity on the measured fracture energy. The results of the experimental and numerical studies showed that metal plasticity increases the fracture energy of the metal‐composite interface as the fracture event progresses. The applied energy truly utilized to propagate metal‐composite interface fracture was predicted numerically by extracting the elastic strain energy data. The predicted true fracture energy was found to be approximately 50% smaller than the experimentally measured average propagation energy. The study concluded that metal plasticity in hybrid DCB configuration overpredicted the experimentally measured fracture energy, and this can be alleviated through numerical methodology such as the finite element approach as presented in this paper.
Immunogenicity and Safety of a Chemically Synthesized Divalent Group A Streptococcal Vaccine
Authors: Wu Yongxiang , Li Suhua , Luo Yanting , Zhao Yunyue , Wang Jiarui , Dong Ruimin , Xie Xujing , Zhu Jieming , Liu Jinlai
Published: February 2018

Background. Group A streptococcus (GAS) infections and poststreptococcal sequelae remain a health problem worldwide, which necessitates searching for an effective vaccine, while no licensed GAS vaccine is available. We have developed a divalent peptide vaccine composed of 84 amino acids to cover the main GAS serotypes (M1 and M12 streptococci) in China, and herein, we aimed to evaluate immunogenicity and safety of this vaccine.Methods. Mice were immunized with the vaccine. ELISA, indirect bactericidal test, and immunofluorescent assay were used to study immunogenicity. GAS challenge assay was used to test the protective effect. Safety was tested by histopathological analysis.Results. Immunized group mice (n=16) developed higher titer antibody after immunization than nonimmunized group mice (n=16) did. This antibody can deposit on the surface of GAS and promote killing of GAS, resulting in 93.1% decrease of M1 GAS and 89.5% of M12 GAS. When challenged with M1 and M12 streptococci, immunized group mice had a higher survival rate (87.5% and 75%) than nonimmunized group mice (37.5% and 25%). No autoimmune reactions were detected on organs of mice.Conclusion. The results suggest that this vaccine shows fair immunogenicity and safety, which will lead our research on GAS vaccine into clinical trial.
Evaluation of the Genotoxic and Antigenotoxic Effects of Andiroba (Carapa guianensis Aublet) Oil and Nanoemulsion on Swiss Mice
Authors: Melo Karina Motta , Fascineli Maria Luiza , Milhomem-Paixão Susana Suely Rodrigues , Grisolia Cesar Koppe , Santos Alberdan Silva , Salgado Hugo Leonardo Crisóstomo , Muehlmann Luis Alexandre , Azevedo Ricardo Bentes , Pieczarka Julio Cesar , Nagamachi Cleusa Yoshiko
Published: February 2018

The Carapa guianensis (andiroba) oil is commonly used by the Amazon population for medicinal purposes. The objective of this study was to determine the genotoxic and antigenotoxic potential of the andiroba oil (AO) and nanoemulsion (AN) using Swiss mice. Therefore, we used the comet assay and micronucleus test. The AO predominant compounds were oleic (39.13%), palmitic (33.22%), and linoleic (16.86%) acids. AN composition obeyed the surfactant/oil ratio of 0.69, and the Tween 80/Span 80 ratio was held at 0.9. Our results showed no cytotoxicity or genotoxicity in the mice treated with AO and AN alone. However, there was a significant reduction in the polychromatic erythrocytes (PCEs) numbers in all groups treated with doxorubicin (DOX), including those pretreated with AO and AN. Thus, the samples tested did not protect against DOX. On the other hand, our results showed a large increase in micronucleus (MN) formation when the mice were treated with DOX alone; these numbers were reduced when the animals were pretreated with AO and AN. The results indicate a protective effect of andiroba on MN formation and show no evidence of genotoxicity in mice.