bluuebunny/crossref_metadata_2025 huggingface dataset:
Nationwide Seroprevalence of Dirofilaria immitis Antigen and Antibodies to Borrelia burgdorferi, Anaplasma spp., and Ehrlichia spp. in Shelter Cats in the United States, 2007–2011
Authors: Smith Rachel C. , Starkey Lindsay A. , Bowles Joy V. , Butler Jamie M. , Mount Jane , Land Tracy M. , Blagburn Byron L.
Published: October 2024

Vector-borne infections persist as a significant issue in both human and animal health. Many of the most common vector-borne infections in the USA, especially tick-borne infections, are known to be zoonotic, including Lyme disease, anaplasmosis, and ehrlichiosis, and these infections may also negatively impact the health of infected animals. Convenient patient-side assays for the detection of antibodies to Borrelia burgdorferi, Anaplasma spp., and Ehrlichia spp., and antigen of Dirofilaria immitis have allowed for the generation of robust and large-scale prevalence data in dogs. Data of similar scale and distribution are not available in cats, and most feline prevalence studies have evaluated a small sample size with limited geographic distribution. The objective of this study was to evaluate the prevalence of antibodies to B. burgdorferi, Anaplasma spp., and Ehrlichia spp., and D. immitis antigen among shelter cats across the USA, a population that is presumably at high risk for ectoparasitism and, consequently, exposure to vector-borne infections. In total, 2232 whole blood samples were collected from shelter cats across four regions of the USA—South, Northeast, Midwest, and West—and were evaluated using the Idexx SNAP® 4Dx® Test. Ectoparasites were also opportunistically collected from cats during blood collection and morphologically identified. The prevalence of at least one vector-borne infection was 2.60%, and the nationwide prevalence was 1.88% for B. burgdorferi, 0.54% for Anaplasma spp., 0.09% for Ehrlichia spp., and 0.55% for D. immitis. A total of 1120 ectoparasites were collected from 423 cats, including 27 ticks and 1093 fleas. Although the overall prevalence of the pathogens in this survey is relatively low, we observe that there is an increased exposure risk regionally for some agents, with geographic distributions in this study mostly coinciding with established human and canine distributions. Understanding these findings in an assumed non-protected population of cats allows us to extrapolate the risk to pet cats if they are not provided routine veterinary care, including a broad-spectrum parasite prevention program.
Radiological Changes in the Spinal Cord and Brain of Patients with HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP)
Authors: Stack Emily H. , Okar Serhat V. , Wu Tianxia , Stack Mallory , Mina Yair , Gaitán María , Azodi Shila , Frazier Will , Ohayon Joan , Cortese Irene C. M. , Reich Daniel S. , Nair Govind , Jacobson Steven
Published: October 2024

HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic, progressive neurological disorder and shares many radiological and clinical features with other more prevalent myelopathies. Here, we quantified spinal cord and brain volumes in adults with HAM/TSP in comparison with healthy volunteers (HVs) and individuals diagnosed with relapsing–remitting or progressive multiple sclerosis (RRMS or P-MS). Clinical disability and MRI were assessed in 24 HVs, 43 HAM/TSP subjects, and 46 MS subjects. Spinal cord cross-sectional area (SCCSA) and brain tissue volumes were measured and compared. HAM/TSP subjects had significantly lower SCCSA corresponding to cervical levels 2 and 3 (C2–3) (54.0 ± 8 mm2), cervical levels 4 and 5 (C4–5) (57.8 ± 8 mm2), and thoracic levels 4 to 9 (T4–9) (22.7 ± 4 mm2) and significantly elevated brain white matter hyperintensity (WMH) fraction (0.004 ± 0.008) compared to the HVs (C2–3: 69.4 ± 8 mm2, C4–5: 75.1 ± 9 mm2, T4–9: 34.1 ± 4 mm2; all p < 0.0001; and WMH: 0.0005 ± 0.0007; p < 0.001). In the HAM/TSP subjects, SCCSA at all levels but not WMH showed a significant correlation with clinical disability scores. WMH in HAM/TSP subjects, therefore, may not be related to clinical disability. SCCSA in our limited RRMS cohort was higher than the HAM/TSP cohort (C2–3: 67.6 ± 8 mm2, C4–5: 72.7 ± 9 mm2, T4–9: 33.4 ± 5 mm2; all p < 0.0001) and WMH was lower than in P-MS subjects (p = 0.0067). Principal component analysis suggested that SCCSA and WMH may be used to differentiate HAM/TSP from MS. Understanding these differences msay help establish early diagnostic criteria for HAM/TSP patients.
Ethogram of the Domestic Cat
Authors: Kappel Isabelle , Riedel Marie-Christin , Becker Fiona , Hicks Shay , Warlich-Zach Nicole , Ganslosser Udo
Published: October 2024

The existing domestic cat literature predominantly contains lists detailing its behaviour. However, these lists are neither specific enough for domestic cats nor general enough for all behaviours on a macro level of the domestic cat. Furthermore, the majority of studies lack illustrations. Therefore, we have developed a comprehensive ethogram of the domestic cat and scalable schematic representations of the behaviour units. We evaluated observational data from free-roaming cats and cats from private households using ad libitum and focal animal sampling (video material 55.31 h, n = 170 animals). In addition, we used the leading literature and detailed anatomical studies to create systematic categories and morphologically correct drawings. A total of 117 discrete behaviours were recorded and divided into 12 categories. The description of each behaviour includes a name, a word definition, an ethological classification and a schematic illustration (excluding the category devoted to vocalisations). An ethogram is needed to better understand the behavioural repertoire of healthy domestic cats and to facilitate the design phase of further investigations. This document can help to better distinguish species-appropriate behaviours and body postures from those behaviours and poses that indicate chronic pain, stress or discomfort.
Random Raman Lasing in Diode-Pumped Multi-Mode Graded-Index Fiber with Femtosecond Laser-Inscribed Random Refractive Index Structures of Various Shapes
Authors: Kuznetsov Alexey G. , Munkueva Zhibzema E. , Dostovalov Alexandr V. , Kokhanovskiy Alexey Y. , Elizarova Polina A. , Nemov Ilya N. , Revyakin Alexandr A. , Kharenko Denis S. , Babin Sergey A.
Published: October 2024

Diode-pumped multi-mode graded-index (GRIN) fiber Raman lasers provide prominent brightness enhancement both in linear and half-open cavities with random distributed feedback via natural Rayleigh backscattering. Femtosecond laser-inscribed random refractive index structures allow for the sufficient reduction in the Raman threshold by means of Rayleigh backscattering signal enhancement by +50 + 66 dB relative to the intrinsic fiber level. At the same time, they offer an opportunity to generate Stokes beams with a shape close to fundamental transverse mode (LP01), as well as to select higher-order modes such as LP11 with a near-1D longitudinal random structure shifted off the fiber axis. Further development of the inscription technology includes the fabrication of 3D ring-shaped random structures using a spatial light modulator (SLM) in a 100/140 μm GRIN multi-mode fiber. This allows for the generation of a multi-mode diode-pumped GRIN fiber random Raman laser at 976 nm with a ring-shaped output beam at a relatively low pumping threshold (~160 W), demonstrated for the first time to our knowledge.
Anticrossing and Mode Coupling in Bent All-Glass Leakage Channel Microstructured Optical Fibers with Large Mode Area
Authors: Denisov Alexander N. , Dvoyrin Vladislav V. , Semjonov Sergey L.
Published: October 2024

This paper presents the results of a detailed theoretical study of the bending properties of original all-glass leakage channel microstructured optical fibers (LC MOFs) over a bending radius range from 3 cm to 11 cm. These LC MOFs contain two layers of fluorine-doped silica glass elements with reduced refractive index, different diameters, and different distances between them. We determined the spatial distributions of the electric field components of different modes in addition to the usual parameters such as effective refractive indices, bending losses, and spatial intensity distributions. A detailed analysis showed that three modes for each polarization have to be considered to correctly calculate the bending losses. Two pairs of these three modes couple in two distinct bending radius ranges, specifically near 3.68 cm and near 5.95 cm, and the mode coupling in these pairs is resonant. The resulting bending losses of the LC MOF for two polarizations are very close to each other and have two maxima at bending radii of 3.68 cm and 5.95 cm. However, the nature of these maxima is not resonant; they are caused by the combined influence of all three modes, each of which has specific dependencies of losses and other parameters on the bending radius that exhibit quasi-resonant behavior near the corresponding bending radii.
A Review: Phase Measurement Techniques Based on Metasurfaces
Authors: Zhao Zhicheng , Hu Yueqiang , Chen Shanyong
Published: October 2024

Phase carries crucial information about the light propagation process, and the visualization and quantitative measurement of phase have important applications, ranging from ultra-precision metrology to biomedical imaging. Traditional phase measurement techniques typically require large and complex optical systems, limiting their applicability in various scenarios. Optical metasurfaces, as flat optical elements, offer a novel approach to phase measurement by manipulating light at the nanoscale through light-matter interactions. Metasurfaces are advantageous due to their lightweight, multifunctional, and easy-to-integrate nature, providing new possibilities for simplifying traditional phase measurement methods. This review categorizes phase measurement techniques into quantitative and non-quantitative methods and reviews the advancements in metasurface-based phase measurement technologies. Detailed discussions are provided on several methods, including vortex phase contrast, holographic interferometry, shearing interferometry, the Transport of Intensity Equation (TIE), and wavefront sensing. The advantages and limitations of metasurfaces in phase measurement are highlighted, and future research directions are explored.
Classicalization of Quantum Mechanics: Classical Radiation Damping Without the Runaway Solution
Authors: Petrosky Tomio , Goto Yuki , Garmon Savannah
Published: October 2024

In this paper, we review a new treatment of classical radiation damping, which resolves a known contradiction in the Abraham–Lorentz equation that has long been a concern. This radiation damping problem has already been solved in quantum mechanics by the method introduced by Friedrichs. Based on Friedrichs’ treatment, we solved this long-standing problem by classicalizing quantum mechanics by replacing the canonical commutation relation from quantum mechanics with the Poisson bracket relation in classical mechanics.
Influence of Foliar Application of Nanoparticles on Low Temperature Resistance of Rice Seedlings
Authors: Ullah Shafi , Ikram Muhammad , Xiao Jian , Khan Atika , Din Ismail , Huang Jianliang
Published: October 2024

Chilling stress, a common abiotic factor, adversely affects the growth and biomass of rice seedlings during the early stages, ultimately reducing the yield. Effective strategies to mitigate these negative impacts are essential for improving rice productivity. The application of nanotechnology in agriculture, particularly nanoparticles (NPs), has shown a promising effect in alleviating chilling stress in plants. This study evaluates the effects of various nanoparticles, ZnO (0, 50, 100, and 200 mg/L), Fe2O3 (0, 50, 75, and 100 mg/L), TiO2 (0, 50, 75, and 100 mg/L), and CeO2 (0, 50, 75, and 100 mg/L) on the chilling resistance with one control (a water spray) under a normal temperature. Four rice cultivars: LLY-7108 and XZX-6 (Low-temperature-tolerant), and LLY-32 and ZJZ-17 (Low-temperature-susceptible) were tested in this experiment. Rice seedlings were subjected to low temperature conditions (12 h light 14 °C/12 h dark, at 10 °C) for five days, followed by seven days of recovery. The results of this study demonstrate that NPs significantly enhanced seedling height fresh/dry weight and root length compared to untreated controls under chilling stress. NP treatment also reduced the reactive oxygen species (ROS), malondialdehyde (MDA), and proline content, while enhancing superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, thereby mitigating oxidative damage. The four rice varieties exhibited clear signs of rapid growth recovery and positive physiological changes due to NPs’ application. Among the tested cultivars, LLY-7108 showed the most substantial recovery and physiological responses, while ZJZ-17 exhibited the least. The findings of this study indicate that the foliar application of ZnO (100 mg/L), Fe2O3 (50 mg/L), TiO2 (50 mg/L), and CeO2 (75 mg/L) NPs effectively mitigates chilling stress in rice seedlings, likely by enhancing the antioxidant enzymatic activity while reducing the oxidative damage. This study highlights the potential of NPs as effective agents in reducing the adverse effects of chilling stress on rice.
Development of a Feasible and Efficient In Vitro Rescue Protocol for Immature Prunus spp. Embryos
Authors: Casanovas Maria , Claveria Elisabet , Dolcet-Sanjuan Ramon
Published: October 2024

The major factors affecting the in vitro immature embryo rescue efficiencies from Prunus persica or P. armeniaca accessions have been identified, along with improving the feasibility. Variations in the woody plant medium (WPM) were used depending on the embryo size. Embryos less than 5 mm long were cultured in WPM supplemented with 1 μM BAP and 1 μM GA3, while embryos bigger than 5 mm long were cultured in hormone-free medium, with or without vermiculite. The environmental in vitro culture conditions consisted of three phases: a (I) stratification at 4 °C during a 3- to 5-month-long period in the dark, followed by (II) growth of germinated embryos at 14 °C for a 4-week-long period, with 12 h light a day, which favors plantlet development, and finally, (III) growth at 24 °C, with 16 h light a day, until the plantlets were acclimatized in the greenhouse. The germination of smaller embryos, at the end of phase I, ranged from 82.2% to 22.1% for apricots and flat peaches, respectively, whereas for bigger embryos, the germination varied from 97.3% to 53.2% for the same species. The embryo germination for peaches and nectarines ranged from 40.1% to 30.3% for smaller embryos, and from 91.9% to 63.0% for bigger embryos. Endo- and epiphytic contamination, affecting from 7.4% to 52.9% of cultured embryos, depending on the fruit type and conservation conditions, and the capacity to acclimate to soil conditions, ranging from 50.4% to 93.2%, were the two most important factors influencing the protocol’s efficiency and feasibility. Considering the overall efficiencies, expressed as hardened plants transferred to field plots over clean uncontaminated embryo, the values ranged from 55.8% for nectarines, 54.0% for peaches, 45.6% for apricots, and 23.3% for flat fruits. The addition of vermiculite to the culture medium significantly improved the plantlet development, avoiding subculture to fresh medium when an extension of phase III was required before acclimatization. Compared to laboratory glassware, the use of food glass containers with air-permeable sealing film, along with vermiculite-containing medium, significantly reduced the costs when handling the large number of embryos required for breeding programs.
Consistory and Youth Surveillance in the Republic of Geneva: The Case of Games (16th–18th Centuries)
Authors: Werly Baptiste
Published: October 2024

This article explores the theme of youth gaming in the Calvinist Republic of Geneva. This investigation is largely based on disciplinary cases found in the registers of the Consistory of Geneva. Legislative sources, and the registers of the Pastor’s Company and the Small Council, are also used herein. This study, deliberately placed at the crossroads of the history of games, childhood, and Calvinist-style ecclesiastical discipline, aims to shed light on the Consistory’s policy of controlling play activities, and to understand what was at stake. The author defends the idea that the surveillance and control of games played by children was intended to prevent them from straying from their religious education, but also to protect them from the excesses and dangers of abusive play and immoderate behavior (violence, lost money, insults, etc.).
Research on Status Assessment and Operation and Maintenance of Electric Vehicle DC Charging Stations Based on XGboost
Authors: Fang Hualiang , Liao Jiaqi , Huang Shuo , Zhang Maojie
Published: October 2024

With the rapid development of electric vehicles, the infrastructure for charging stations is also expanding quickly, and the failure rate of charging piles is increasing. To address the effective operation and maintenance of charging stations, a method based on the XGBoost algorithm for electric vehicle DC charging stations is proposed. An operation and maintenance system is constructed based on state analysis, considering the operational status of the charging stations and users’ charging habits. Factors such as driving and charging habits, road traffic, and charging station equipment are taken into account. The training sample data are established using historical data, online monitoring data, and external environmental data, and the charging station status evaluation model is trained using the XGBoost algorithm. Based on the condition assessment results, a risk assessment model is established in combination with fault parameters. Risk tracking of the charging stations is conducted using the energy not charged (ENC), evaluating the risk level of each station and determining the operation and maintenance order. The optimal operation and maintenance model for DC charging stations, aimed at achieving both economic and reliability goals, is constructed to determine the operation and maintenance schedule for each station. The results of the case study demonstrate that the state evaluation and operation and maintenance strategy can significantly improve the reliability of the system and the overall benefits of operation and maintenance while meeting the required standards.
Post-Detention Migration in Romania: Reasons, Challenges and Solutions for Preventing Recidivism and Ensuring Reintegration into Society
Authors: Chasciar Vasile , Chasciar Denisa Ramona , Coman Claudiu , Toderici Ovidiu Florin , Toader Liviu , Bularca Maria Cristina
Published: October 2024

This study looks at post-detention migration and focuses on the reasons, challenges and solutions for preventing recidivism and ensuring reintegration into society. This research involves an analysis of the factors influencing the intention to emigrate of criminally sanctioned persons in Romania, the differences between the types of crimes and their impact on the desire to leave the country, as well as the causes of external migration, such as unattractive jobs and inadequate income at the level of the family of origin. The results show that people who have committed crimes against a person are more inclined to emigrate compared to those who have committed crimes against property. Unattractive jobs and insufficient income are also significant factors contributing to the decision to emigrate. This study proposes solutions to facilitate the reintegration of these people into Romanian society and reduce recidivism rates.
Mindfulness Approach and the Redefined Analysis Model of Conflict the Case Study of the Ukraine Conflict
Authors: Nguyen Manh-Cuong , Chelabi Kaddour , Anjum Safia , Kumari Sushma , Samoylenko Svitlana , Silwizya Kangwa , Nghiem Tran
Published: October 2024

To address the gaps in previous studies, this paper explores the integration of mindfulness approaches in understanding and tackling the Ukraine conflict. While existing research has predominantly focused on traditional theoretical frameworks like constructivism, neoliberalism, and realism, the potential insights offered by a mindfulness approach remain largely unexplored. This study aims to elucidate how mindfulness principles such as compassion, empathy, and non-judgmental awareness can offer valuable perspectives and effective strategies for resolving conflict and fostering peace in Ukraine. By critically analysing the complexities and dynamics of the conflict, the paper transforms the traditional ABC triangle of conflict into an expanded ABCM diamond of conflict, incorporating a mindfulness approach. Through expert interviews and an analysis of secondary data sources, the paper contributes to a deeper understanding of conflict resolution techniques and offers novel approaches to addressing contemporary geopolitical challenges such as the Ukraine conflict.
Empirical Inferences Under Bayesian Framework to Identify Cellwise Outliers
Authors: Sartore Luca , Chen Lu , Bejleri Valbona
Published: October 2024

Outliers are typically identified using frequentist methods. The data are classified as “outliers” or “not outliers” based on a test statistic that measures the magnitude of the difference between a value and the majority part of the data. The threshold for a data value to be an outlier is typically defined by the user. However, a subjective choice of the threshold increases the uncertainty associated with outlier status for each data value. A cellwise outlier detection algorithm named FuzzyHRT is used to automate the editing process in repeated surveys. This algorithm uses Bienaymé–Chebyshev’s inequality and fuzzy logic to detect four different types of outliers resulting from format inconsistencies, historical, tail, and relational anomalies. However, fuzzy logic is not suited for probabilistic reasoning behind the identification of anomalous cells. Bayesian methods are well suited for quantifying the uncertainty associated with the identification of outliers. Although, as suggested by the literature, there exist well-developed Bayesian methods for record-level outlier detection, Bayesian methods for identifying outliers within individual records (i.e., at the cell level) remain unexplored. This paper presents two approaches from the Bayesian perspective to study the uncertainty associated with identifying outliers. A Bayesian bootstrap approach is explored to study the uncertainty associated with the output scores from the FuzzyHRT algorithm. Empirical likelihoods in a Bayesian setting are also considered for probabilistic reasoning behind the identification of anomalous cells. NASS survey data for livestock and major crop yield (such as corn) are considered for comparing the performances of the two proposed approaches with recent cellwise outlier methods.
Thermal Stress as a Critical Factor in the Viability and Duration of Spittlebug Eggs
Authors: Duarte Milena , Calsavara Luís Augusto , Auad Alexander Machado
Published: October 2024

The spittlebug Mahanarva spectabilis (Distant, 1909) (Hemiptera: Cercopidae) is an important pest that causes significant losses in the production of forage crops for cattle feed. Information on the thermal requirements of this insect during the egg stage is crucial in assessing the interaction between insects and forage. The aim of this research was to evaluate the effects of constant and oscillating (diurnal/nocturnal) temperatures on the viability of M. spectabilis eggs and the duration of the egg stage. Temperatures of 20 °C to 30 °C were ideal for the development of this insect pest, resulting in greater viability and faster development of the embryos. In addition, it should be noted that a variation of up to 8 days is feasible for synchronizing the phenological stages of the forage plants and the eggs to be laid on these plants when subjected to 30 °C (16.6 days) or 20 °C (25.7 days) without significantly altering the viability of the eggs. Notably, a temperature oscillation of 25 °C during the day and 15 °C at night increased the viability of the eggs after exiting diapause. These results are essential for the rearing of M. spectabilis in the laboratory, allowing for the supply of eggs for experiments and contributing to advances in studies aimed at developing effective integrated management strategies for this pest.
Special Issue: Fixed-Point Theory and Its Applications, Dedicated to the Memory of Professor William Arthur Kirk
Authors: Zaslavski Alexander J.
Published: October 2024

Fixed-point theory is a rapidly growing area of research [...]
Fe3O4/Mulberry Stem Biochar as a Potential Amendment for Highly Arsenic-Contaminated Paddy Soil Remediation
Authors: Tang Ziling , Liang Meina , Ding Yanmei , Liu Chongmin , Zhang Qing , Wang Dunqiu , Zhang Xuehong
Published: October 2024

Magnetite-loaded biochar has recently received attention owing to its ability to remove arsenic from contaminated soil. In this study, mulberry stem biochar (MBC) and Fe3O4-loaded mulberry stem biochar (Fe3O4@MBC) were produced and used in a 100-day incubation experiment to investigate their performance in the stabilization of arsenic in paddy soil severely polluted by the As (237.68 mg·kg−1) mechanism. Incubation experiments showed that Fe3O4@MBC was more effective in immobilizing As after incubation for 100 days. Moreover, adding Fe3O4@MBC facilitated the transformation of exchangeable heavy metals into organic-bound and residual forms, thereby reducing As available concentrations, mobility, and bioavailability in the soil, and elevating slightly the soil pH and dissolved organic carbon (DOC). The concentration of TCLP-extractable As (AsTCLP) in contaminated soil was reduced from 93.85 to 7.64 μg·L−1 within 10 d, below the safety limit for drinking water set by the World Health Organization (WHO). The characterization results of Fe3O4@MBC after incubation indicated that the mechanisms for As passivation are linked to redox reactions, complexation, electrostatic attraction, surface adsorption, and coprecipitation. Conclusively, Fe3O4@MBC is a promising amendment in highly As-contaminated soil and provides a theoretical reference in such polluted paddy soil remediation.
Differences in Cytokine Expression at Baseline and in Response to Mineral Stimulation by Peripheral Blood Mononuclear Cells from Podoconiosis Cases and Healthy Control Individuals
Authors: Negash Mikias , Girma Tigist , Chanyalew Menberework , Alemayehu Dawit H. , Alcantara Diana , Davey Gail , Boyton Rosemary J. , Altmann Daniel M. , Newport Melanie J. , Howe Rawleigh
Published: October 2024

Epidemiological, histological, and immunogenetic studies suggest that podoconiosis (a non-infectious tropical lymphoedema affecting approximately 4 million people globally) is an HLA class II-associated inflammatory condition that develops in response to an unknown trigger found in volcanic red clay soils. Silicate particles of the kaolinite and aluminum types have been identified in femoral lymph node biopsy samples from endemic area residents, suggesting a possible role in the pathogenesis of podoconiosis. We measured in vitro peripheral blood mononuclear cell cytokine responses (TNF-α, IL-1β, and IFN-γ) to stimulation with the minerals kaolinite, chlorite, and beryllium sulfate (all at 100 µM) using ELISA. Real time PCR was used to measure gene expression of signature cytokines in fresh whole blood, comparing podoconiosis patients and endemic healthy controls. Our results showed that the levels of TNF-α and IL-1β from in vitro cell cultures were significantly higher in unstimulated samples from patients compared to controls (p = 0.04 and p = 0.005, respectively). The minerals kaolinite and chlorite induced two and three-fold higher levels of IL-1β following 24 h of stimulation in healthy controls compared to patients, respectively. We did not find significant differences in mRNA expression of the cytokine genes assayed, though a slight fold increment in IL-1β and TGF-β was observed. In conclusion, our data suggest that the immune system is in a state of persistent activation in vivo in podoconiosis patients, and additional studies of immune regulation and exhaustion are needed to further characterize immune dysfunction in the pathogenesis of the disease. A better understanding of the underlying processes could lead to the development of a ‘biosignature’ detectable in the early reversible stages that could ultimately contribute to the elimination of this preventable, disabling, neglected tropical disease.
Second-Generation Phage Lambda Platform Employing SARS-CoV-2 Fusion Proteins as a Vaccine Candidate
Authors: Catala Alexis , Davenport Bennett J. , Morrison Thomas E. , Catalano Carlos E.
Published: October 2024

The recent SARS-CoV-2 (COVID-19) pandemic exemplifies how newly emerging and reemerging viruses can quickly overwhelm and cripple global infrastructures. Coupled with synergistic factors such as increasing population densities, the constant and massive mobility of people across geographical areas and substantial changes to ecosystems worldwide, these pathogens pose serious health concerns on a global scale. Vaccines form an indispensable defense, serving to control and mitigate the impact of devastating outbreaks and pandemics. Towards these efforts, we developed a tunable vaccine platform that can be engineered to simultaneously display multiple viral antigens. Here, we describe a second-generation version wherein chimeric proteins derived from SARS-CoV-2 and bacteriophage lambda are engineered and used to decorate phage-like particles with defined surface densities and retention of antigenicity. This streamlines the engineering of particle decoration, thus improving the overall manufacturing potential of the system. In a prime-boost regimen, mice immunized with particles containing as little as 42 copies of the chimeric protein on their surface develop potent neutralizing antibody responses, and immunization protects mice against virulent SARS-CoV-2 challenge. The platform is highly versatile, making it a promising strategy to rapidly develop vaccines against a potentially broad range of infectious diseases.
Delineation of Groundwater Potential Zones Using Geospatial Techniques. Case Study: Roman City and the Surrounding Area in the Northeastern Region, Romania
Authors: Bogdan Petrut-Liviu , Nedeff Valentin , Panainte-Lehadus Mirela , Chitimuș Dana , Barsan Narcis , Nedeff Florin Marian
Published: October 2024

Effective groundwater management is crucial under the current climatic conditions, addressing both qualitative and quantitative aspects. An important step in delineating groundwater potential zones involves remote sensing (RS) data and geographic information systems (GISs), facilitating resource assessment, and the implementation of suitable field data management. This study introduces the delineation of potential groundwater zones using seven layers and the Multi-Criteria Decision Analysis (MCDA) method. Satty’s Analytic Hierarchy Process (AHP) was employed to rank the seven selected parameters, contributing to the advancement of groundwater research and resource assessment. All seven thematic layers (Rainfall, Geology, Land Use/Land Cover, Drainage Density, Elevation, Slope, and Soil) were prepared and analyzed to delineate groundwater potential zones. The resulting groundwater potential zone map was categorized into four classes, Very Good, Good, Moderate, and Poor, covering areas of 81.53 km2 (45.1%), 56.36 km2 (31.2%), 19.54 km2 (10.8%), and 23.17 km2 (12.8%) of the total area, respectively. The accuracy of the output was validated by comparing it with information on groundwater prospects in the area, and the overall accuracy of the method was approximately 72%. High-yield boreholes were drilled and concentrated in the Very Good groundwater potential zones, while low-yield ones were developed in the Poor areas.
Hydrochemical Characterisation of the Basement Aquifer with a Focus on the Origin of Nitrate in the Highly Urbanised Niamey Region, SW of Niger
Authors: Wannous Manal , Osenbrück Karsten , Tanimoun Bachir , Koeniger Paul
Published: October 2024

This study investigated the basement aquifer beneath the urbanised city of Niamey and the agricultural fields of Kollo, SW of Niger. During the observation period spanning from 2021 to 2023, groundwater and surface water samples were collected for analysis of major ions and the stable isotopes oxygen-18 and deuterium (δ18O and δ2H) of water. To trace the origin of high nitrate concentrations (NO3−) found in several observation and drinking water wells in both areas, δ15N and δ18O isotope values of NO3− were analysed in groundwater and eluted soil samples. The observed hydrochemical patterns mainly reflect the heterogeneity of the weathered fringe of the basement aquifer. Decreasing concentrations of NO3− and δ18O and δ2H values were observed in relation to the distance of the Niger River and increasing thickness of the clay layer on the surface. The wells close to the river in Niamey show a dilution effect during the flood season, and the NO3− concentrations displayed a continuous increasing trend. The δ15N-NO3 and δ18O-NO3 values confirmed that septic tank water is spreading in the region of Niamey and that manure originating from livestock in Kollo is the main source of NO3−. The patterns of δ15N in the soil samples coincide with those of cattle’s manure spread in both areas. The shallow wells show significantly higher values of electric conductivity and NO3− concentrations compared to the deeper wells, which clearly indicates the influence of shallow septic tanks on water quality.
Exploring the Link Between Adverse Childhood Experiences and Adolescent Dating Violence Perpetration and Victimization: A Review of the Literature
Authors: Russell Katie N. , Voith Laura A. , Withrow Ashley S.
Published: October 2024

Background and Purpose: Adverse childhood experiences (ACEs) have been linked with multiple adverse outcomes, including adolescent dating violence (ADV). ADV is a serious public health issue, with the U.S. rates ranging from 6.4% to 73% for victimization across all types and from 11% to 77% for perpetration. Given the high prevalence of ADV and the fact that it has been frequently linked with ACEs earlier in life, it is crucial to thoroughly understand this relation and apply this information to prevention and intervention efforts. However, to date, there are no known reviews synthesizing the link between ACEs and ADV, nor exploring which ACEs have been included across other studies. Methods: To address these gaps, this study included three comprehensive searches to identify the following: (1) the measurement tools used to measure ACEs; (2) the type of ACEs significantly linked with ADV; and (3) the risk and protective factors considered in this relation. Results: A total of 34 studies were included. There were 24 different assessment tools used to measure the ACEs of adolescents, with the number of ACEs included ranging from 1 to 93. All but one study linked at least one ACE to ADV. The types of ACEs linked with ADV consisted of victimization (e.g., child abuse and bullying), witnessing violence (i.e., home and school), and household dysfunction (e.g., caregiver substance abuse). Several risk and protective factors were considered. Conclusions/Implications: This study found the measurement of ACEs in the literature to be inconsistent, making it difficult to synthesize and compare the findings across studies. Despite the measurement inconsistencies, however, most studies linked ACEs with ADV, emphasizing the importance of considering this relation in prevention/intervention programming.
Impact of the clinically oriented roles of a general practice receptionist: a systematic review with narrative synthesis
Authors: Ban Keigo , Greenfield Sheila , Burrows Michael , Gale Nicola , Litchfield Ian
Published: October 2024

Background

Modern general practice is characterised by increased demand and growing multidisciplinarity, including ring-fenced funding for additional non-clinical roles. For practice receptionists, however, training has remained unchanged for decades despite primary care being under greater pressure than ever, with receptionists becoming a growing focal point for abuse and unprecedented numbers leaving the role.

Aim

To present the evidence of the range of tasks that receptionists continue to perform, describing their impact on primary care delivery and how the role might be better supported.

Design and setting

Systematic review of research conducted in the UK.

Method

A systematic review of evidence contained in the major medical databases (MEDLINE/PubMed, CINAHL, ASSIA, Cochrane Library, and Embase) from January 2000 to March 2024 was conducted, including hand searches of the bibliographies of included studies.

Results

In total, 29 studies were identified that grouped into three themes: service delivery, patient attitudes, and receptionist experience. The theme ‘service delivery’ confirms the continuing role of receptionists in providing administrative support alongside the clinical tasks of prioritising patients for consultations, facilitating repeat prescriptions, and communicating blood test results. The theme ‘patient attitudes’ describes how patients lacked trust in receptionists, who were viewed as unqualified and unnecessarily obstructive. Finally, in considering receptionist experience, the contrast between their confidence in performing administrative roles and the anxiety induced from the clinically related tasks was described, particularly the mounting pressure from patients to meet their preferences for clinician appointments.

Conclusion

Although confident performing administrative tasks, receptionists described uncertainty and anxiety when providing clinically oriented support or managing patients when their requests for appointments could not be met. More appropriate training or professionalisation might improve staff retainment.
العوامل المؤثرة في استخدام الإنترنت في شراء تذاكر السفر
Authors: مسعود عماد يوسف , عربيات محمد أحمد
Published: October 2024

هدفت الدراسة إلى تحديد العوامل المؤثرة في استخدام الإنترنت في شراء تذاكر السفر، وإلى تقديم مجموعة من التوصيات للشركات لزيادة اهتمامها بالعوامل التي تؤثر في شراء تذاكر السفر، وما سيكون له من أثر على زيادة نسبة شراء تذاكر السفر بواسطة الإنترنت، وانعكاسه على تقليل التكاليف لدى هذه الشركات. وكانت عينة الدراسة مكونة من المسافرين عبر الملكية الأردنية؛ حيث تم توزيع ٤٥٠ استبانه، وكانت عدد الاستبانات المستردة والصالحة للتحليل ٣٥٨ استبانه بنسبة ٧٩٪. وخلصت الدراسة إلى أن هناك أثراً للفائدة من استخدام الإنترنت، وخصائص المواقع الإلكترونية، ومخاطر استخدام الإنترنت، والبحث عن المعلومات على شراء تذاكر السفر، وعدم وجود تأثير لمهارات استخدام الإنترنت على شراء تذاكر السفر، وكان لعاملي الفائدة وخصائص المواقع التأثير الأكبر في استخدام الإنترنت في شراء تذاكر السفر، بينما كان تأثير (المخاطر، والبحث عن المعلومات) قليلا.
سلوك تسوق السعوديين عن طريق الإنترنت
Authors: الدين أحمد صدر , باهزيك ياسر
Published: October 2024

كشفت دراسة حديثة باستخدام الإنترنت من خلال ٢٣١ شخصاً قاموا بتعبئة استبانة في المملكة العربية أن الساعات التي يقضونها على الإنترنت واستخدام وسائل الإعلام الاجتماعية مرتفعة جدا بالمقارنة باستخدام التسوق عبر الإنترنت. وتستخدم شبكة الإنترنت بشكل رئيسي للمعاملات التي هي أقل تعقيدا مثل الأعمال المصرفية، وحجوزات السفر، وشراء وتحميل الأفلام والموسيقى. وهناك نسبة كبيرة من الذكور لمستخدمي الكمبيوتر، خريجي الجامعات، غير المتزوجين (العزاب)، وبعض من الإناث للقيام بالتسوق عن طريق الإنترنت. وهذا يشبه الدراسات التي أجريت في الدول الصناعية والحديثة باستثناء أن هذا البحث يشمل في دراسته على غير المتزوجين والنساء. وقد ظهر أن المخاوف الرئيسية التي تضمنها هذا البحث تتمثل في عدم ثقة السعوديين في الإنترنت وقلة حماية مستخدمي الإنترنت.
Quantitative evaluation of the canalis sinuosus relative to adjacent structures in cone-beam computed tomography images
Authors: Jabali Sahar , Pishva Sajjad , Bardal Roghieh , Bahrami Farough , Mostafavi Maryam
Published: October 2024

Background. Careful anatomical investigation of canalis sinuosus (CS) is essential to prevent damage to blood vessels and nerves in this area during surgical procedures, such as placing dental implants in the anterior maxillary region. This study investigated the relationship and distance between the CS and its adjacent structures. Methods. A total of 400 cone-beam computed tomography (CBCT) images of Iranian adults aged 20–86 years were included in this retrospective study. Two observers assessed all the images twice with a time interval of one month. The closest tooth to the CS, its position relative to the CS, and distance measurements of the CS from adjacent structures were determined. Results. CS was found in 10.5% of all images. The mean diameter of the canal was 1.06±0.29 mm, which was not significantly different between the age groups, right and left sides, or genders. The most common location of CS was mid-position relative to the upper lateral incisors. In linear measurements, only the distance from the CS to the buccal cortical plate and perpendicular to the nasopalatine canal exhibited a significant difference between the two sexes, with no significant difference between the right and left sides. Conclusion. CS location was significantly more palato-lateral in males. There was no significant difference in the prevalence between the two sexes.
Biological behavior of mesenchymal stem cells on two types of commercial dermal scaffolds: An in vitro study
Authors: Moghaddas Omid , Seyedjafari Ehsan , Mahoutchi Donya Sadat
Published: October 2024

Background. Acellular dermal matrix (ADM) has been introduced as an alternative to autogenous grafts. This study assessed the biological behavior of mesenchymal stem cells (MSCs) on two types of commercial ADM scaffolds. Methods. The present in vitro study investigated the behavior of MSCs cultured on scaffold type I CenoDerm® (Tissue Regeneration Corporation) and type II Acellular Dermis (Iranian Tissue Product Co.) as the test groups and an empty well plate as the control group (n=78). Cell attachment was assessed after 12 hours of incubation using 6,4-diamidino-2-phenylindole (DAPI) staining and methyl thiazole tetrazolium (MTT) assay. Cell proliferation was assessed using the MTT assay at 24- and 84-hour and 7-day intervals. Cell morphology was also assessed under a scanning electron microscope (SEM) at 24 hours. MTT assay and DAPI staining were repeated for five samples in all the three groups. Mann-Whitney, ANOVA, and post hoc Tukey tests were used for statistical analysis. Results. The DAPI staining and MTT assay showed similar results concerning cell attachment between all the groups at 12 hours (P=0.4). At 24 hours, cell proliferation was significantly higher in scaffold groups (P<0.001). At seven days, the lowest cell proliferation was noted in the scaffold II group, with a significant difference between the groups (P=0.01). At 24 hours, cell expansion was greater in the control group, followed by the scaffold I group. Conclusion. Both scaffolds were similar in MSC attachment, but scaffold I appeared superior to scaffold II in terms of MSC proliferation and morphology in vitro.
Comparison of the effect of albumin with platelet-rich fibrin (Alb-PRF) gel and hyaluronic acid gel injection on interdental papilla reconstruction: A randomized clinical trial
Authors: Vadiati Saberi Bardia , Khalighi Sigaroudi Ali , Kamani Mobina , Rafiei Elahe
Published: October 2024

Background. The interdental papilla plays a crucial role in the esthetic of the smile. Papilla reconstruction surgery is one of the most unpredictable periodontal procedures. This study compared the effect of the non-surgical application of a commercial hyaluronic acid (HA) gel with an autogenous gel named "albumin with platelet-rich fibrin" (Alb-PRF) on interdental papilla reconstruction. Methods. This trial was conducted on 46 incomplete interdental papillae with class I or II defects. The papillae were randomly divided into two experimental groups, and commercial HA or Alb-PRF (prepared from the patient’s peripheral blood) was injected into the papillae twice at a 21-day interval. Three and six months after the intervention, photographs were taken from the black triangles and the black surfaces compared to each other. Patient satisfaction and dental plaque index were also evaluated at the end of the study. Results. The black triangles’ surfaces significantly decreased over time in both experimental groups (P<0.001). However, there were no significant differences between the two groups (P=0.994). The intervention resulted in the patients’ relative satisfaction with the treatment results in both groups. However, the difference between the two groups was not statistically significant (P=0.965). In addition, no statistically significant differences were observed in plaque index between the two groups (P=0.566). Conclusion. HA or Alb-PRF injection into the incomplete interdental papilla may restore the soft tissue to a great extent and lead to patient satisfaction by reducing the area of black triangles that threaten esthetics; however, more studies are needed.
Effect of orthodontic forced eruption for implant site development in the maxillary esthetic zone: A systematic review of clinical data
Authors: Talebi Ardakani Mohammadreza , Kheiri Aida , Torabzadeh Majid , Mahmoudian Amirhosein , Talebi Mohammad Hossien , Talebi Amir
Published: October 2024

Dental implant placement in the esthetic zone is associated with challenges for clinicians. The best esthetic outcome of this procedure can be obtained through precise management of hard and soft tissue. Orthodontic forced eruption (OFE) has presented an alternative approach to augment hard and soft tissues, which can be applied rapidly or slowly. OFE of hopeless teeth with its periodontal attachment results in a favorable implant preparation site. Therefore, the present systematic review evaluated the effect of implant site preparation using OFE in hopeless teeth. A complete electronic search was performed in PubMed/MEDLINE, Scopus, and Google Scholar from June 2020 to November 2023. The search was limited to clinical English language studies. Studies were excluded if OFE was performed without implant placement. Finally, 15 studies with a total of 21 teeth, all located in the maxillary anterior region, were included in this study. In eight studies, bone grafting procedures were performed before implant placement. Using OFE could rapidly prepare the implant site by enhancing hard and soft tissues. However, additional interventions like guided bone regeneration should be considered case-dependent.
Early Palliative Care in the Emergency Department: A Concept Clarification
Authors: Counts Kelly , Lasiter Sue
Published: October 2024

Introduction: Healthcare advances have contributed to patients living longer with chronic illnesses and diseases with uncertain trajectories impacting quality of life (QOL). Palliative care (PC) is no longer only for dying oncology patients as many healthcare practitioners have adopted the PC concept in diverse care settings and the timing of PC implementation remains ambiguous. There is a need to develop an operational definition of early palliative care (EPC) by clarifying the phenomenon and bridging concepts with empirical data to develop and test possible interventions before integrating EPC into emergency care (EC). Methods: Norris’ concept clarification method was used as the philosophical framework to define, analyze, and clarify EPC. An electronic search of literature from 2000-2024, using CINAHL, PubMed, APA PsychINFO, and Psychology and Behavioral Sciences Collection databases and search terms "early palliative care" AND "emergency care" NOT "animals", and NOT "pediatrics" were screened for eligible articles. Results: Of the 826 articles identified; 22 articles were retained for review. Attributes included timing, palliative, and EC; antecedents included symptom burden, access to care, and cognitive awareness; consequences included QOL and resource utilization; an empirical referent used to screen patients is the highly accurate surprise question "Would I be surprised if this patient died within a year?" Conclusion: Clarifying the concept of EPC leading to an operational definition will advance the development of interventions that support the implementation of EPC in ED clinical practice.