bluuebunny/crossref_metadata_2025 huggingface dataset:
Human motor neurons derived from induced pluripotent stem cells are susceptible to SARS-CoV-2 infection
Authors: Cappelletti Gioia , Colombrita Claudia , Limanaqi Fiona , Invernizzi Sabrina , Garziano Micaela , Vanetti Claudia , Moscheni Claudia , Santangelo Serena , Zecchini Silvia , Trabattoni Daria , Silani Vincenzo , Clerici Mario , Ratti Antonia , Biasin Mara
Published: December 2023

Introduction

COVID-19 typically causes Q7 respiratory disorders, but a high proportion of patients also reports neurological and neuromuscular symptoms during and after SARSCoV-2 infection. Despite a number of studies documenting SARS-CoV-2 infection of various neuronal cell populations, the impact of SARS-CoV-2 exposure on motor neuronal cells specifically has not been investigated so far.

Methods

Thus, by using human iPSC-derived motor neurons (iPSC-MNs) we assessed: (i) the expression of SARS-CoV-2 main receptors; (ii) iPSC-MN infectability by SARS-CoV-2; and (iii) the effect of SARS-CoV-2 exposure on iPSC-MN transcriptome.

Results

Gene expression profiling and immunofluorescence (IF) analysis of the main host cell receptors recognized by SARS-CoV-2 revealed that all of them are expressed in iPSC-MNs, with CD147 and NRP1 being the most represented ones. By analyzing SARS-CoV-2 N1 and N2 gene expression over time, we observed that human iPSC-MNs were productively infected by SARS-CoV-2 in the absence of cytopathic effect. Supernatants collected from SARS-CoV-2-infected iPSC-MNs were able to re-infect VeroE6 cells. Image analyses of SARS-CoV-2 nucleocapsid proteins by IF confirmed iPSC-MN infectability. Furthermore, SARS-CoV-2 infection in iPSCMNs significantly altered the expression of genes (IL-6, ANG, S1PR1, BCL2, BAX, Casp8, HLA-A, ERAP1, CD147, MX1) associated with cell survival and metabolism, as well as antiviral and inflammatory response.

Discussion:

These results suggest for the very first time that SARS-CoV-2 can productively infect human iPSC-derived MNs probably by binding CD147 and NRP1 receptors. Such information will be important to unveil the biological bases of neuromuscular disorders characterizing SARS-CoV-2 infection and the so called long-COVID symptoms.
Postural control deficits due to bilateral pyramidal tract lesions exemplified by hereditary spastic paraplegia (HSP) originate from increased feedback time delay and reduced long-term error corrections
Authors: Dalin Daniela , Wiesmeier Isabella Katharina , Heimbach Bernhard , Weiller Cornelius , Maurer Christoph
Published: December 2023

Pyramidal tract lesions determine the clinical syndrome of Hereditary Spastic Paraplegia (HSP). The clinical impairments of HSP are typically exemplified by their deficits in mobility, leading to falls and injuries. The first aim of this study was to identify the cause for postural abnormalities caused by pyramidal tract lesions in HSP. The second aim was to specify the effect of treadmill training for postural abnormalities. We examined nine HSP patients before and after treadmill training, as well as nine healthy control subjects during perturbed and unperturbed stance. We found that HSP was associated with larger sway amplitudes and velocities. Body excursions following platform tilts were larger, and upper body excursions showed a phase lead. Model-based analysis detected a greater time delay and a reduced long-term error correction of postural reactions in the center of mass. HSP patients performed significantly better in clinical assessments after treadmill training. In addition, treadmill training reduced sway amplitudes and body excursions, most likely by increasing positional and velocity error correction gain as a compensatory mechanism, while the time delay and long-term error correction gain remained largely unaffected. Moreover, the upper body’s phase lead was reduced. We conclude that HSP leads to very specific postural impairments. While postural control generally benefits from treadmill training, the effect seems to mainly rely on compensatory mechanisms, whereas the original deficits are not affected significantly.
Functional force stimulation alters motor neuron discharge patterns
Authors: Xu Yaodan , Du Zuyu , Cheng Anyi , Lin Runwei , Wang Kaichen , Jin Yibin , Dai Chenyun , Xu Lin
Published: December 2023

Introduction

Beneficial effects have been observed for mechanical vibration stimulation (MVS), which are mainly attributed to tonic vibration reflex (TVR). TVR is reported to elicit synchronized motor unit activation during locally applied vibration. Similar effects are also observed in a novel vibration system referred to as functional force stimulation (FFS). However, the manifestation of TVR in FFS is doubted due to the use of global electromyography (EMG) features in previous analysis. Our study aims to investigate the effects of FFS on motor unit discharge patterns of the human biceps brachii by analyzing the motor unit spike trains decoded from the high-density surface EMG.

Methods

Eighteen healthy subjects volunteered in FFS training with different amplitudes and frequencies. One hundred and twenty-eight channel surface EMG was recorded from the biceps brachii and then decoded after motion-artifact removal. The discharge timings were extracted and the coherence between different motor unit spike trains was calculated to quantify synchronized activation.

Results and discussion

Significant synchronization within the vibration cycle and/or its integer multiples is observed for all FFS trials, which increases with increased FFS amplitude. Our results reveal the basic physiological mechanism involved in FFS, providing a theoretical foundation for analyzing and introducing FFS into clinical rehabilitation programs.
A controlled lumbar puncture procedure improves the safety of lumbar puncture
Authors: Li Chun , Li Miaomiao , Wang Yixian , Li Shaoyi , Cong Lin , Ma Weining
Published: December 2023

Background

In order to improve the safety of lumbar puncture (LP), we designed a new type of LP needle, that is, an integrated and controlled LP needle, which can actively and accurately control the flow rate and retention of cerebrospinal fluid (CSF) during puncture, so as to achieve a controlled LP procedure.

Objective

To evaluate whether a controlled LP procedure can improve the comfort of LP and reduce the risk of complications associated with LP.

Methods

Patients requiring LP (n = 63) were pierced with an integrated and controlled LP needle or a conventional LP needle. The differences in vital signs, symptom score, comfort, operation time, CSF loss, CSF pressure fluctuation and back pain before and after puncture were analyzed.

Results

An integrated and controlled LP needle (n = 35) significantly improved patients’ headache symptoms before and after puncture. In addition, a controlled LP procedure significantly reduced the amount of unnecessary CSF loss (p < 0.001), shortened the time of puncture (p < 0.001), improved patient comfort (p = 0.001) and reduced the incidence of back pain (p < 0.001). For patients with high intracranial pressure (HICP), the fluctuations in pressure of the CSF were also reduced while obtaining similar amounts of CSF (p = 0.009).

Conclusion

A controlled LP procedure avoids unnecessary CSF loss, prevents rapid fluctuations in CSF pressure in patients with HICP, and reduces the risks associated with LP.
Acute consumption of a branched chain amino acid and vitamin B-6 containing sports drink does not improve multiple sprint exercise performance, but increases post-exercise blood glucose
Authors: Farra Saro D.
Published: December 2023

Purpose

The aim of this study was to investigate the ergogenicity of BioSteel High Performance Sports Drink (B-HPSD), a commercially available branched chain amino acid (BCAA) and vitamin B-6 (VitB-6) supplement, on multiple sprint exercise (MSE).

Methods

Eleven experienced cyclists completed two MSE trials in counterbalanced order, after ingesting either B-HPSD (2,256 mg of BCAA, 300 mcg of VitB-6) or placebo (PLA). The MSE protocol consisted of five maximal effort 1 km sprints on a cycle ergometer separated by 2 min of active recovery. Power output (PO) was continuously measured throughout the cycling protocol. Heart rate (HR) and ratings of perceived exertion (RPE) were monitored following each sprint. Capillary blood samples were collected and analyzed for lactate and glucose before and 2 min post-trial. Cognitive function was assessed before and 15 min after the exercise protocol.

Results

The PO maintained during each 1 km sprint decreased throughout the protocol (p < 0.05), but the change in PO was similar between conditions. Post-exercise blood glucose was elevated after consuming B-HPSD but not PLA (p < 0.05). Blood lactate (p < 0.05), HR (p < 0.05) and RPE (p < 0.05) increased throughout the trials, however no differences were observed between conditions. Cognitive performance improved after exercise (p < 0.05), but the change was similar between conditions.

Conclusion

These results demonstrate that acute B-HPSD consumption does not have an ergogenic effect on MSE performance. However, ingestion of B-HPSD increased post-exercise blood glucose concentration when compared to PLA.
Inverse association between isoflavones and prediabetes risk: evidence from NHANES 2007–2010 and 2017–2018
Authors: Zhou Yanjun , Qin Shaolei , Zhu Yan , Xu Peng , Gu Ke
Published: December 2023

Introduction

Prediabetes is a metabolic condition characterized by blood glucose levels that are higher than normal but do not meet the threshold for a diabetes diagnosis. Individuals with prediabetes are at an increased risk of developing type 2 diabetes and associated complications. However, limited epidemiological studies have investigated the association between flavonoids from plant-based diets and the risk of prediabetes, and the existing evidence from these studies is inconsistent.

Methods

Therefore, we utilized data from 19,021 participants (mean age: 32.03 years) in the National Health and Nutrition Examination Survey (NHANES) conducted during 2007–2010 and 2017–2018 to investigate the potential association between dietary flavonoid intake and prediabetes risk by weighted logistic regression analysis. Furthermore, the data from 3,706 participants (mean age: 35.98 years) from NHANES 2007–2010 were used to assess the correlation between concentrations of isoflavones and their metabolites in urine and prediabetes risk by weighted logistic regression analysis.

Results

Our findings revealed an inverse association between the intake of glycitein (OR: 0.88; 95% CI: 0.82–0.96; p = 0.003), genistein (OR: 0.98; 95% CI: 0.97–0.99; p = 0.004), daidzein (OR: 0.98; 95% CI: 0.96–0.99; p = 0.009), and total isoflavones (OR: 0.99; 95% CI: 0.98–1.00; p = 0.005) with the risk of prediabetes. Moreover, we observed an inverse association between the concentration of daidzein in urine (OR: 0.84; 95% CI: 0.73–0.96; p = 0.012) and the concentration of genistein in urine (OR:0.83; 95% CI: 0.75–0.93; p = 0.003) with the risk of prediabetes using weighted logistic regression.

Conclusion

In conclusion, our findings suggest a potential protective effect of isoflavones against the development of prediabetes.
Associations of oxidative balance score with total abdominal fat mass and visceral adipose tissue mass percentages among young and middle-aged adults: findings from NHANES 2011–2018
Authors: Wang Kai , Deng Minggang , Wu Jinyi , Luo Lingli , Chen Rui , Liu Fang , Nie Jiaqi , Tao Fengxi , Li Qingwen , Luo Xin , Xia Fang
Published: December 2023

Objective

This study aimed to explore the association of the oxidative balance score (OBS) with total abdominal fat mass (TAFM) and visceral adipose tissue mass (VATM) percentages among young and middle-aged U.S. adults.

Methods

Young and middle-aged adults in the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2018 were included. Analysis of variance and Rao-Scott adjusted chi-square tests were used to compare the characteristics across quartiles of OBS. Univariate and multivariate weighted logistic regression models were employed to explore the relationship between OBS and the risks of high TAFM or high VATM percentage in the general population and subgroups, while the interaction effects were tested with a likelihood test. Weighted restricted cubic spline analyses were utilized to assess the non-linear association of OBS with TAFM and VATM percentages.

Results

The final sample included 8,734 young and middle-aged non-institutionalized U.S. adults representing 134.7 million adults. Compared with adults in the first quartile of OBS, those with higher OBS were less likely to have a high TAFM percentage; the ORs and 95% CI for adults in the second, third, and highest quartiles of OBS were 0.70 (0.53–0.94), 0.49 (0.36–0.60), and 0.25 (0.18–0.36), respectively. Similar trends were observed in the association between OBS and VATM percentages. Moreover, similar effects were confirmed in the sensitivity analyses and subgroup analyses according to demographic characteristics. Regarding the OBS subclass, higher dietary OBS and lifestyle OBS were also correlated with decreased ORs of high TAFM and VATM percentages.

Conclusion

This study strongly suggests that higher OBS, as well as higher dietary OBS and lifestyle OBS, are significantly correlated with lower risks of abdominal obesity and visceral fat accumulation. The findings highlight the importance of an antioxidant-rich diet and maintaining a healthy lifestyle in reducing the risks.
Case Report: Lenvatinib for the treatment of recurrent hepatocellular carcinoma in people living with HIV: a report of two cases
Authors: Morsica Giulia , Bertoni Costanza , Hasson Hamid , Messina Emanuela , Uberti Foppa Caterina
Published: December 2023

The use and choice of the best systemic treatment is gaining increasing interest in people living with HIV (PLWH) because hepatocellular carcinoma (HCC) represents an increasing cause of morbidity and mortality in this setting and most HCCs are diagnosed in the advanced stage. Ten years ago, the multi-kinase inhibitor lenvatinib was approved in the first-line setting. However, to date, no data on the efficacy and tolerability of lenvatinib in PLWH from clinical trials and real-life studies are available. Case 1 was a gentleman with hepatitis B virus–related cirrhosis who underwent orthotopic liver transplantation for HCC and developed peritoneal metastasis several years later. Lenvatinib treatment was selected at HCC recurrence. This participant maintained undetectable HIV viremia and a relatively preserved immune status during 6 months of systemic treatment with lenvatinib. After 6 months, he discontinued lenvatinib for progression of the disease (growing of peritoneal metastasis) and uncontrolled hypertension. Case 2 was a gentleman with hepatitis C–genotype 1a–related cirrhosis who experienced unresectable recurrences after radiofrequency thermal ablation of the tumor. At the first recurrence, HCC was treated with six cycles of trans-catheter arterial chemoembolization; at the second recurrence, the participant underwent trans-catheter arterial radioembolization; and at the third recurrence, he received lenvatinib. A week after the start of lenvatinib, the participant had liver decompensation and discontinued therapy. The presently reported cases showed low tolerability of systemic therapy with lenvatinib in PLWH. Cumulative data are necessary to define the position of lenvatinib in this setting.
Long-term disease-free survival following comprehensive involved site radiotherapy for oligometastases
Authors: Kao Johnny , Sahagian Michelle , Gupta Vani , Missios Symeon , Sangal Ashish
Published: December 2023

Introduction

Despite recent advances in drug development, durable complete remissions with systemic therapy alone for metastatic cancers remain infrequent. With the development of advanced radiation technologies capable of selectively sparing normal tissues, patients with oligometastases are often amenable to comprehensive involved site radiotherapy with curative intent. This study reports the long-term outcomes and patterns of failure for patients treated with total metastatic ablation often in combination with systemic therapy.

Materials and methods

Consecutive adult patients with oligometastases from solid tumor malignancy treated by a single high volume radiation oncologist between 2014 and 2021 were retrospectively analyzed. Oligometastases were defined as 5 or fewer metastatic lesions where all sites of active disease are amenable to local treatment. Comprehensive involved site radiotherapy consisted of stereotactic radiotherapy to a median dose of 27 Gy in 3 fractions and intensity modulated radiation therapy to a median dose of 50 Gy in 15 fractions. This study analyzed overall survival, progression-free survival, patterns of failure and toxicity.

Results

A total of 130 patients with 209 treated distant metastases were treated with a median follow-up of 36 months. The 4-year overall survival, progression-free survival, local control and distant control was 41%, 23%, 86% and 29%. Patterns of failure include 23% alive and free of disease (NED), 52% distant failure only, 9% NED but death from comorbid illness, 7% both local and distant failure, 4% NED but lost to follow-up, 4% referred to hospice before restaging, 1% local only failure, 1% alive with second primary cancer. Late grade 3+ toxicities occurred in 4% of patients, most commonly radionecrosis.

Conclusion

Involved site radiotherapy to all areas of known disease can safely achieve durable complete remissions in patients with oligometastases treated in the real world setting. Distant failures account for the majority of treatment failures and isolated local failures are exceedingly uncommon. Oligometastases represents a promising setting to investigate novel therapeutics targeting minimal residual disease.
A rare adverse effects of COVID-19 vaccine in a patient with a latent tumor: A case report and literature review
Authors: Xu Wenjing , Nian Weiqi
Published: December 2023

The 2019 novel coronavirus infection has done significant damage to the world. The effectiveness and safety of the vaccine, the most critical measure to control the epidemic, has attracted attention. In this case, we report the diagnosis and treatment of a rare patient with adverse effects of the COVID-19 vaccine who had G6PD deficiency by genetic tests. We discuss the possible impact of G6PD deficiency on COVID-19 infection and potential vaccine adverse effects. Patients with severe G6PD deficiency should be monitored for vaccine safety. This article may complement a rare mechanism of vaccine side effects and chemotherapy-related side effects.
Chaga mushroom: a super-fungus with countless facets and untapped potential
Authors: Fordjour Eric , Manful Charles F. , Javed Rabia , Galagedara Lakshman W. , Cuss Chad W. , Cheema Mumtaz , Thomas Raymond
Published: December 2023

Inonotus obliquus (Chaga mushroom) is an inexpensive fungus with a broad range of traditional and medicinal applications. These applications include therapy for breast, cervix, and skin cancers, as well as treating diabetes. However, its benefits are virtually untapped due to a limited understanding of its mycochemical composition and bioactivities. In this article, we explore the ethnobotany, mycochemistry, pharmacology, traditional therapeutic, cosmetic, and prospective agricultural uses. The review establishes that several secondary metabolites, such as steroids, terpenoids, and other compounds exist in chaga. Findings on its bioactivity have demonstrated its ability as an antioxidant, anti-inflammatory, antiviral, and antitumor agent. The study also demonstrates that Chaga powder has a long history of traditional use for medicinal purposes, pipe smoking rituals, and mystical future forecasts. The study further reveals that the applications of Chaga powder can be extended to industries such as pharmaceuticals, food, cosmetics, and agriculture. However numerous publications focused on the pharmaceutical benefits of Chaga with few publications on other applications. Overall, chaga is a promising natural resource with a wide range of potential applications and therefore the diverse array of therapeutic compounds makes it an attractive candidate for various applications such as plant biofertilizers and active ingredients in cosmetics and pharmaceutical products. Thus, further exploration of Chaga’s potential benefits in agriculture and other industries could lead to exciting new developments and innovations.
A cross-sectional study of appropriateness evaluation of anticoagulation therapy for inpatients with nonvalvular atrial fibrillation
Authors: Zheng Xiao-Yuan , Feng Guang-Wei , Guo Jing , Xie Fen , Li Xia , Zhang Ming-Zhu , Zhang Xiu-Fen , Wu Xiu-Feng , Ding Yong-Juan
Published: December 2023

Background: Oral anticoagulants (OACs) are essential for stroke prevention in patients with nonvalvular atrial fibrillation (NVAF). However, the appropriateness of anticoagulation treatment in locally practice remains unclear. This study evaluated compliance with anticoagulation therapy concerning the guidelines and drug labels in patients with NVAF.

Methods: Hospitalized patients diagnosed with NVAF between 1 November 2020, and 31 December 2021, were retrospectively enrolled. The appropriateness of anticoagulation regimens at discharge was evaluated based on a flowchart designed according to atrial fibrillation (AF) guidelines and medication labels. Furthermore, we explored factors potentially influencing the “no-use of OACs” using binary logistic regression and verified anticoagulation-related issues through a physician questionnaire.

Results: A total of 536 patients were enrolled in this study, including 254 patients (47.4%) with inappropriate anticoagulation regimens. 112 patients (20.9%) were categorized as “underdosing-use of OACs,” 134 (25%) who needed anticoagulation therapy were “no-use of OACs” and eight (1.5%) were “over-use of OACs.” The results of a binary logistic regression analysis showed that paroxysmal AF (odds ratio [OR], 7.74; 95% confidence interval [CI], 4.57–13.10), increased blood creatinine levels (OR, 1.88; 95% CI, 1.11–3.16), hospitalized pacemaker implantation (OR, 6.76; 95% CI, 2.67–17.11), percutaneous coronary intervention (OR, 3.35; 95% CI, 1.44–7.80), and an increased HAS-BLED score (OR, 1.62; 95% CI, 1.11–2.35) were associated with “no-use of OACs” in patients with NVAF who had indications for anticoagulation therapy.

Conclusion: For patients with NVAF with severe renal dysfunction and paroxysmal AF, anticoagulation therapy was inadequate. The underdosing-use of OACs in patients with NVAF was frequently observed. We recommend an anticoagulation management team to tailor anticoagulation regimens to suit each patient’s needs.
Brief isoflurane administration as an adjunct treatment to control organophosphate-induced convulsions and neuropathology
Authors: Puthillathu Narayanan , Moffett John R. , Korotcov Alexandru , Bosomtwi Asamoah , Vengilote Ranjini , Krishnan Jishnu K. S. , Johnson Erik A. , Arun Peethambaran , Namboodiri Aryan M.
Published: December 2023

Organophosphate-based chemical agents (OP), including nerve agents and certain pesticides such as paraoxon, are potent acetylcholinesterase inhibitors that cause severe convulsions and seizures, leading to permanent central nervous system (CNS) damage if not treated promptly. The current treatment regimen for OP poisoning is intramuscular injection of atropine sulfate with an oxime such as pralidoxime (2-PAM) to mitigate cholinergic over-activation of the somatic musculature and autonomic nervous system. This treatment does not provide protection against CNS cholinergic overactivation and therefore convulsions require additional medication. Benzodiazepines are the currently accepted treatment for OP-induced convulsions, but the convulsions become refractory to these GABAA agonists and repeated dosing has diminishing effectiveness. As such, adjunct anticonvulsant treatments are needed to provide improved protection against recurrent and prolonged convulsions and the associated excitotoxic CNS damage that results from them. Previously we have shown that brief, 4-min administration of 3%–5% isoflurane in 100% oxygen has profound anticonvulsant and CNS protective effects when administered 30 min after a lethal dose of paraoxon. In this report we provide an extended time course of the effectiveness of 5% isoflurane delivered for 5 min, ranging from 60 to 180 min after a lethal dose of paraoxon in rats. We observed substantial effectiveness in preventing neuronal loss as shown by Fluoro-Jade B staining when isoflurane was administered 1 h after paraoxon, with diminishing effectiveness at 90, 120 and 180 min. In vivo magnetic resonance imaging (MRI) derived T2 and mean diffusivity (MD) values showed that 5-min isoflurane administration at a concentration of 5% prevents brain edema and tissue damage when administered 1 h after a lethal dose of paraoxon. We also observed reduced astrogliosis as shown by GFAP immunohistochemistry. Studies with continuous EEG monitoring are ongoing to demonstrate effectiveness in animal models of soman poisoning.
3D digital holographic polarimetry of diffuse optically anisotropic biological tissue object fields
Authors: Ushenko Alexander , Zheng Jun , Gorsky Mykhaylo , Dubolazov Alexander , Ushenko Yuriy , Soltys Iryna , Mikirin Ivan , Chen Zhebo , Wanchuliak Oleh , Gordey Ivan , Jingxian Cai
Published: December 2023

The experimental validation of methods for the digital holographic 3D layer-by-layer reconstruction of complex amplitude distributions in different phase planes of diffuse biological tissue histological section microscopic images with a subsequent reconstruction of azimuth and ellipticity polarization maps is presented. Polarization dependencies of the integral and layer-by-layer vector structure of the laser object field azimuth and ellipticity distributions in two types of biological layer samples—the fibrous myocardium and parenchymal liver—have been investigated. Scenarios and dynamics of changes in the values of statistical moments of the first to fourth orders, which characterize the integral and layer-by-layer polarization maps of diffuse biological tissue histological section microscopic images with different morphological structures, have been determined. Criteria for selecting the polarization components of the object field that are formed by acts of scattering of different multiplicities have been identified. An example of biomedical application in cancer detection of biological tissues is provided.
High temperature inhibits photosynthesis of chrysanthemum (Chrysanthemum morifolium Ramat.) seedlings more than relative humidity
Authors: Zhou Jianfei , Jiang Xiaodong , Agathokleous Evgenios , Lu Xiaojing , Yang Zaiqiang , Li Ruiying
Published: December 2023

High relative humidity (RH) and high temperature are expected more frequently due to climate change, and can severely affect the growth of chrysanthemums. In order to analyze the interactive effects of RH and high temperature on the photosynthetic performance of chrysanthemum, a completely randomized block experiment was conducted with three factors, namely temperature (Day/night temperature, 35°C/18°C, 38°C/18°C, 41°C/18°C), RH (Whole day RH, 50%, 70%, 90%), and treatment duration (3d, 6d, 9d). The control (CK) temperature was 28°C/18°C and RH was 50%. The results showed that with the increase of temperature, the apparent quantum efficiency (AQE), maximum net photosynthetic rate (Pn-max), net photosynthetic rate (Pn), transpiration rate (Tr), water use efficiency (WUE), maximal recorded fluorescence intensity (Fm), PSII maximal photochemical efficiency (Fv/Fm), absorption flux per cross section (ABS/CSm), trapped energy flux per cross section (TRo/CSm), electron transport flux per cross section (ETo/CSm) and photosynthetic pigment content of leaves significantly decreased, the minimal recorded fluorescence intensity (Fo), fluorescence intensity at point J of the OJIP curve (Fj) and non-photochemical quenching per cross section (DIo/CSm) significantly increased, the fluorescence difference kinetics of the OJ phase of chrysanthemum leaves showed K-bands. Pn, AQE, Fm, Fv, Fv/Fm, ABS/CSm, TRo/CSm, ETo/CSm and photosynthetic pigment content were higher at 70% RH than the other two RH conditions. The dominant factor causing the decrease of Pn in leaves was stomatal limitation at 35°C,38°C, three RH conditions, 3d and 6d, but non-stomatal limitation at 41°C and 9d. There was an interaction between temperature and RH, with a significant impact on Pn. The temperature had the greatest impact on Pn, followed by RH. This study confirms that heat stress severely affects the photosynthesis of chrysanthemum leaves, and when the temperature reaches or exceeds 35°C, adjusting the RH to 70% can effectively reduce the impact of heat stress on chrysanthemum photosynthesis.
Playfulness from children’s perspectives: development and validation of the Children’s Playfulness Scale as a self-report instrument for children from 3 years of age
Authors: Duss Isabelle , Rüdisüli Cornelia , Wustmann Seiler Corina , Lannen Patricia
Published: December 2023

Children’s playfulness refers to children’s enjoyment, motivation, and engagement in play and has been predominantly assessed from an adult perspective. To assess children’s perspectives on their own playfulness, we adapted and modified the Children’s Playfulness Scale (CPS) for children from 3 years and used a two-level response format with a total of four answer options. We tested the self-report scale with 564 children between 3 and 8 years of age who attended childcare center or kindergarten. Results indicated that the adapted version of the CPS identified the five distinct domains of playfulness: social spontaneity, cognitive spontaneity, physical spontaneity, sense of humor, and manifest joy; furthermore, results showed invariance across multiple groups for gender, age, and language skills. A highly significant positive correlation was found between children’s self-reported playfulness and children’s self-reported social self-concept (r = 0.54, p < 0.001), which demonstrates convergent validity. No association was found with teacher proxy report of children’s playfulness (r = 0.03, p = 0.92). Overall, our study confirmed first indications of the validity of the modified CPS as a reliable instrument for assessing children’s self-reported playfulness. This enables children as young as 3 years old to be assessed on their own playfulness, which is a valuable supplement to the adult perspective.
The impact of videogames on the mood of amateur youth players during consecutive games
Authors: Mateo-Orcajada Adrián , Vaquero-Cristóbal Raquel , Gallardo-Guerrero Ana María , Abenza-Cano Lucía
Published: December 2023

Introduction

Esports have experienced tremendous growth in recent years. In the scientific field, previous research has shown the determining role of psychology in competitive performance, but little is known about the factors that may be more determinant. In addition, in the amateur field, where fun and enjoyment are the most important factors, it has been observed that players can see their psychological state altered due to different factors, but it is not known if the outcome of the game (win or lose) can be influential. For this reason, the aim of the present investigation was to analyze changes in players' mood between three consecutive games as a function of the outcome of each game.

Methods

A total of 14 amateur players participated in the research, all of them with previous experience and being regular League of Legends players. The participants completed the POMS questionnaire before the start of each game and the outcome of each game was recorded at the end.

Results

The results showed that no significant pre-game differences were found in any of the games, regardless of winning or losing. Significant differences were found in the pre-game mood between the first and second game, according to the outcome of the first game, and between the second and third game, according to the outcome of the second game. Between the first and second games, there was a significant increase in depression (p = 0.038) and anger (p = 0.003) when the first game was lost; and between the second and third games, there was a decrease in tension (p = 0.003) and anger (p = 0.022) when the second game was won. In addition, it should be noted that fatigue increased significantly after each game, regardless of the outcome, and with respect to the change in mood, this was more noticeable when the first game was lost and the second was won, as significant changes were observed in tension (p = 0.028), depression (p = 0.030) and anger (p = 0.006).

Conclusion

Pre-match mood does not influence post-match performance, but mood changes do occur between successive matches depending on the outcome of the match.
Case studies of fifteen novel species successfully aided with the use of a veterinary teletriage service
Authors: Ireifej Shadi J. , Krol Justin
Published: December 2023

The veterinary medical field is constantly growing and evolving. Embracing the growth of readily available video conferencing, and potentially spurred by events such as the COVID-19 pandemic causing the public to seek alternatives to physical contact for medical advice at their local veterinary clinic, the use of long-distance advice or telehealth is a rapidly developing field in its own right. Here we present case studies using a teletriage service, VetTriage, to provide health care advice to clients with underserved species including presenting complications, actions taken during the session, medical advice given, and follow-up of the case when possible. In addition to the everyday difficulty of accessing rapid medical care in recent years, there are households with underserved animal groups such as exotics (small mammals, reptiles, birds, fish, etc.), found wildlife, and in some areas of the country, large animals (horses, cows, etc.). Teletriage services have the potential to reach these underserved animal groups providing a vital service where otherwise no help may be available.
Responses of Soil Respiration to the Interactive Effects of Warming and Drought in Alfalfa Grassland on the Loess Plateau
Authors: Li Jiaxuan , Zhang Jingui , Ma Tao , Lv Wenqiang , Shen Yuying , Yang Qian , Wang Xianzhi , Wang Ruobing , Xiang Qian , Lv Long , Zhang Jianjun , Ma Jingyong
Published: December 2023

Elevated temperature and frequent drought events under global climate change may seriously affect soil respiration. However, the underlying mechanism of the effects of warming and drought on soil respiration is not fully understood in the context of the Loess Plateau. This study examined the response of soil respiration (Rs) to multiple factors, including warming (W), drought (P), and their interaction (WP), in the semi-arid grassland of the Loess Plateau in Northwest China. The research period was from May to November 2022, with an open-top heating box used for warming and a rain shelter used for drought. The results showed the following: (1) Rs ranged from 1.67 μmol m−2 s−1 to 4.77 μmol m−2 s−1, with an average of 3.36 ± 0.07 μmol m−2 s−1. The cumulative soil carbon flux ranged from 500.97 g C·m−2 to 566.97 g C·m−2, and the average cumulative soil respiration was 535.28 ± 35.44 g C·m−2. (2) Warming increased Rs by 5.04 ± 3.11%, but drought inhibited Rs by 3.40 ± 3.14%, and the interaction between warming and drought significantly reduced soil respiration by 11.27 ± 3.89%. (3) The content of particulate organic carbon (POC), dissolved organic carbon (DOC), soil organic carbon (SOC), and readily oxidized carbon (ROC) decreased with the increased soil depth. ROC after W and WP treatments was significantly higher than that of the control, and POC after P treatment was significantly higher than CK (p < 0.05). (4) The seasonal variation of soil respiration was positively correlated with soil temperature, soil water content, plant height, and leaf area index (p < 0.05), but the response rules differed during different regeneration periods. Soil water content; soil water content and leaf area index; and soil water content, soil temperature, and leaf area index were the factors that regulated the variation in soil respiration in the first, second, and third regeneration periods, respectively. These results clearly showed the limiting effect of drought stress on the coupling between temperature and soil respiration, especially in semi-arid regions. Collectively, the variations in soil respiration under warming, drought, and their interactions were further regulated by different biotic and abiotic factors. Considering future warming, when coupled with increased drought, our findings indicate the importance of considering the interactive effects of climate change on soil respiration and its components in arid and semi-arid regions over the next decade.
The Interaction Effects of Drought–Flood Abrupt Alternation on Rice Yield and Dry Matter Partitioning
Authors: Gao Yun , Hu Tiesong , Li Ping , Qi Xuebin
Published: December 2023

The frequent occurrence of drought–flood abrupt alternation (DFAA) seriously affects crop yield. It is particularly important to explore the dynamics of material accumulation and distribution under DFAA stress to analyze the mechanism of yield formation. In this study, a bucket experiment with DFAA stress groups, drought control (DC) groups, flood control (FC) groups, and normal irrigation (CK) groups was set up from the jointing to the heading stage of rice to analyze the interaction effects of DFAA stress on rice yield and dry matter partitioning. The results showed that compared with the CK group, the average yield reduction rate of rice in the DFAA groups was 23.03%, and the number of grains per panicle, total grain number, thousand-seed mass, and seed setting rate decreased. Compared with the DC groups, the DFAA groups had a significant reduction in yield and its components during the flooding period. Compared with the FC groups, the DFAA groups showed a compensation phenomenon in the yield and its components during the drought period. From the end of DFAA stress to the harvest period, the root partitioning index (PI) of the DFAA groups decreased, the stem PI increased first and then decreased, the leaf PI decreased, and the panicle PI increased. The results showed that the rice leaves increased and thickened, and the stems thickened under DFAA conditions to enhance the ability to resist drought and flooding stress, but the panicle rate was reduced, the growth period of rice was delayed, and the redundant growth of stems and leaves was increased. It is suggested that the depth and duration of stagnant water storage during the flood period of DFAA should be controlled, and the transfer and supply of photosynthetic products to grains should be increased to avoid serious yield reductions. The research results provide a theoretical basis for the rational development of farmland DFAA mitigation measures.
Effects of a Bentonite Clay Product and a Preservative Blend on Ileal and Fecal Nutrient Digestibility in Pigs Fed Wheat Naturally Contaminated with Deoxynivalenol
Authors: Shin Seung Youp , Yoo Seung Bin , Song Yoon Soo , Park Noa , Kim Beob Gyun
Published: December 2023

The objectives were to determine the effects of dietary deoxynivalenol (DON) on apparent ileal digestibility (AID) of nutrients and to evaluate the efficacy of a bentonite (BEN) and a preservative blend (PB) product for alleviating DON effects on the nutrient digestibility of pigs. Twelve crossbred barrows with an initial body weight of 69.4 kg (standard deviation = 3.5) equipped with a T-cannula in the distal ileum were allotted a triplicated 4 × 2 incomplete Latin square design with four dietary treatments and two periods. Dietary treatments were (1) an uncontaminated diet, (2) a contaminated diet (CD) mainly based on contaminated wheat with 1.6 mg/kg DON, (3) CD + 0.25% PB consisting of preservation components as major sources, antioxidants, microorganisms, and amino acids (AA), and (4) CD + 0.25% BEN. The AID and ATTD of dry matter, organic matter, crude protein, most minerals, and most AA were not affected by DON contamination. Dietary DON decreased the AID and ATTD of sodium (p < 0.05) but were restored by supplementing the PB product (p < 0.05). The AID of zinc was increased (p < 0.05) by dietary DON, but supplementing BEN decreased zinc digestibility (p < 0.05). The AID of Arg, Ile, Thr, and Asp was decreased (p < 0.05) by BEN addition. In conclusion, dietary DON affected the digestibility of some minerals but not AA in pigs. Supplemental BEN can negatively affect the nutrient digestibility of some minerals and AA in pigs. The addition of a PB product in pig diets can restore digestibility of sodium but not of other nutrients. Based on these observations, feed additives for alleviating DON effects on nutrient digestibility of pigs can be carefully selected by swine diet formulators.
An Improved Ensemble-Strategy-Assisted Wind Speed Prediction Method for Railway Strong Wind Warnings
Authors: Liu Jian , Cui Xiaolei , Cheng Cheng , Jiang Yan
Published: December 2023

Reliable short-term wind speed prediction is one of the core technologies in the strong wind warning system for railway applications, which is of great significance for ensuring the safety of high-speed train operations and ancillary railway facilities. To improve forecasting accuracy, decomposition-based methods have attracted extensive attention due to their superior ability to address complex data characteristics (e.g., nonstationarity and nonlinearity). Currently, there are two pre-processing schemes for decomposition-based methods, i.e., one-time decomposition and real-time decomposition. In order to apply them better, this paper first expounds the difference between them, based on a combination of DWT (discrete wavelet transform) and CKDE (conditional kernel density estimation). The results show that although the one-time decomposition-based method has an unexceptionable accuracy, it only can provide offline prediction and thus may not be practical. The real-time decomposition-based method possesses stronger practicability and is able to provide online prediction, but it has limited accuracy. Then, an improved ensemble strategy is developed by optimizing the selection of appropriate decomposed components to conduct the prediction on the basis of real-time decomposition. This improved ensemble strategy provides an effective guidance for this selective combination, including taking historical information into consideration in the data. Finally, numerical examples and practicality analysis using two groups of measured wind speed data demonstrate that the proposed method is effective in providing high-precision online wind speed prediction. For example, compared with CKDE, the average degrees of improvement achieved by the proposed method in terms of MAE, RMSE, and MRPE, are 16.25%, 17.66%, and 16.93, respectively, while those compared with the traditional real-time decomposition method are 17.11%, 18.54%, and 16.84, respectively.
Enhanced Blast Response Simulation of LG Panels Using an Elasto-Damage Model with the Finite Element Method
Authors: Elbelbisi Ahmed , Elsisi Alaa , Saffarini Mohammed H. , Salim Hani , Chen Zhen
Published: December 2023

Laminated glass (LG) windows significantly enhance building safety due to their ability to retain shattered glass within the interlayer, but their susceptibility to failure under blast loading remains a concern. Compared with simplified models, detailed constitutive modeling is essential to evaluate these complex scenarios, as experimental investigation faces limitations in spatial and temporal resolutions. This study presents a robust model-based simulation approach for predicting the brittle failure response of glass in blast-resistant LG windows. An elasto-damage relation for glass (EDG) was integrated with the finite element model (FEM) to predict the blast response. Validation against shock tube testing results was performed to ensure the reliability of the FEM. Material parameters for the polymeric interlayer were obtained through dynamic experiments, enabling a reasonable representation of its constitutive behavior using the Johnson–Cook (JC) model. Additionally, a numerical parametric study was conducted to investigate how different glass types influence blast resistance performance. Tempered glass stood out for its blast resistance compared with annealed and heat-strengthened glass, displaying superior strength against blast loads. The Rankine-based elasto-damage description provides a more precise representation of the failure response than commonly used approaches. These findings contribute to advancing model-based simulation approaches for designing better blast-resistant LG windows, ensuring safer buildings.
Effect of Geometrical Confinement on Ordering of Thermoplastic Polyurethanes with Crystallizable Hard and Soft Blocks
Authors: Abukaev Ainur F. , Gorbunova Marina A. , Anokhin Denis V. , Ivanov Dimitri A.
Published: December 2023

A series of multi-block thermoplastic polyurethanes incorporating different soft block structures was synthesized. This was achieved using a poly(butylene adipate) oligomer combined with its macrodiols of both an aromatic and aliphatic nature. The composition of the hard block included 1,6-hexamethylene diisocyanate, 4,4′-diphenylmethane diisocyanate, and 1,4-butanediol. For the first time, the structural evolution and phase composition of both the hard and soft segments were analyzed during in situ thermal treatments. A combination of synchrotron small- and wide-angle X-ray scattering, differential scanning calorimetry, thermogravimetric analysis, and Fourier transform infrared spectroscopy was used to determine the influence of the macrodiol’s nature and crystallization conditions on the polymorphic behavior of poly(butylene adipate). Using a new synthesis scheme, a relatively high degree of crystallinity for urethane blocks was achieved, which depended on the diisocyanate type in the structure of the soft segment. The hard segment domains imposed geometrical constraints on poly(butylene adipate), thereby altering its crystallization process compared to the neat oligomer. Thus, crystallization after annealing at a low temperature (80 °C) was fast, predominantly yielding a metastable β-phase. When heated to 180 °C, which was higher than the hard segment’s melting temperature, a phase-separated structure was observed. Subsequent crystallization was slower, favoring the formation of the stable α-PBA modification. The phase separation could be observed even after the hard block melting. Notably slow crystallization from an isotropic melt was documented after the disruption of phase separation at 230 °C.