bluuebunny/crossref_metadata_2025 huggingface dataset:
Imaging of the Spinal Cord in Multiple Sclerosis: Past, Present, Future
Authors: Chen Yongsheng , Haacke Ewart Mark , Bernitsas Evanthia
Published: November 2020

Spinal cord imaging in multiple sclerosis (MS) plays a significant role in diagnosing and tracking disease progression. The spinal cord is one of four key areas of the central nervous system where documenting the dissemination in space in the McDonald criteria for diagnosing MS. Spinal cord lesion load and the severity of cord atrophy are believed to be more relevant to disability than white matter lesions in the brain in different phenotypes of MS. Axonal loss contributes to spinal cord atrophy in MS and its degree correlates with disease severity and prognosis. Therefore, measures of axonal loss are often reliable biomarkers for monitoring disease progression. With recent technical advances, more and more qualitative and quantitative MRI techniques have been investigated in an attempt to provide objective and reliable diagnostic and monitoring biomarkers in MS. In this article, we discuss the role of spinal cord imaging in the diagnosis and prognosis of MS and, additionally, we review various techniques that may improve our understanding of the disease.
Assessment of HER2 Protein Overexpression and Gene Amplification in Renal Collecting Duct Carcinoma: Therapeutic Implication
Authors: Costantini Manuela , Amoreo Carla Azzurra , Torregrossa Liborio , Alì Greta , Munari Enrico , Jeronimo Carmen , Henrique Rui , Petronilho Sara , Capitanio Umberto , Lucianò Roberta , Suardi Nazareno , Landi Maria Teresa , Anceschi Umberto , Brassetti Aldo , Fazio Vito Michele , Gallucci Michele , Simone Giuseppe , Sentinelli Steno , Poeta Maria Luana
Published: November 2020

Collecting duct carcinoma (CDC) is rare and aggressive histology of kidney cancers. Although different therapeutic approaches have been tested, the 2-year survival remains very poor. Since CDC exhibits overlapping features with urothelial carcinoma, the analysis of shared molecular alterations could provide new insights into the understanding of this rare disease and also therapeutic options. We collected 26 CDC cases, and we assessed HER2 protein expression by immunohistochemistry (IHC) and gene amplification by fluorescence in-situ hybridization (FISH) according to 2018 ASCO/CAP HER2-testing recommendations. Six out of twenty-six (23%) tumors showed HER2 positive staining. In particular, 3+ score was present in 2/6 cases (33%), 2+ in 3/6 cases (50%) and 1+ in 1/6 cases (17%). The 6 HER2+ tumors were also analyzed by FISH to assess gene copy number. One out of six CDC with IHC 3+ was also HER2 amplified, showing an average HER2 copy number ≥4.0 (10.85) and a HER2/CEP17 ratio ≥ (5.63), while the 5/6 cases were HER2 negative. Based on the 2018 ASCO/CAP guidelines overall, 2/26 CDC cases (8%) were HER2+. The present study provides evidence for testing, in future studies, HER2 to assess its clinical value as a novel target for the treatment of this highly malignant cancer.
Brain Metastasis from Unknown Primary Tumour: Moving from Old Retrospective Studies to Clinical Trials on Targeted Agents
Authors: Balestrino Roberta , Rudà Roberta , Soffietti Riccardo
Published: November 2020

Brain metastases (BMs) are the most common intracranial tumours in adults and occur up to 3–10 times more frequently than primary brain tumours. BMs may be the cause of the neurological presenting symptoms in patients with otherwise previously undiagnosed cancer. In up to 15% of patients with BMs, the primary tumour cannot be identified. These cases are known as BM of cancer of unknown primary (CUP) (BM-CUP). CUP has an early and aggressive metastatic spread, poor response to chemotherapy, and poor prognosis. The pathogenesis of CUP seems to be characterized by a specific underlying pro-metastatic signature. The understanding of BM-CUP, despite its relative frequency and unfavourable outcome, is still incomplete and clear indications on management are missing. Advances in diagnostic tools, molecular characterization, and target therapy have shifted the paradigm in the approach to metastasis from CUP: while earlier studies stressed the importance of finding the primary tumour and deciding on treatment based on the primary diagnosis, most recent studies focus on the importance of identifying targetable molecular markers in the metastasis itself. The aim of this review is to summarize current evidence on BM-CUP, from the diagnosis and pathogenesis to the treatment, with a focus on available studies and ongoing clinical trials.
Physiological Roles of ERM Proteins and Transcriptional Regulators in Supporting Membrane Expression of Efflux Transporters as Factors of Drug Resistance in Cancer
Authors: Ogihara Takuo , Mizoi Kenta , Kamioka Hiroki , Yano Kentaro
Published: November 2020

One factor contributing to the malignancy of cancer cells is the acquisition of drug resistance during chemotherapy via increased expression of efflux transporters, such as P-glycoprotein (P-gp), multidrug resistance-associated proteins (MRPs), and breast cancer resistance protein (BCRP). These transporters operate at the cell membrane, and are anchored in place by the scaffold proteins ezrin (Ezr), radixin (Rdx), and moesin (Msn) (ERM proteins), which regulate their functional activity. The identity of the regulatory scaffold protein(s) differs depending upon the transporter, and also upon the tissue in which it is expressed, even for the same transporter. Another factor contributing to malignancy is metastatic ability. Epithelial–mesenchymal transition (EMT) is the first step in the conversion of primary epithelial cells into mesenchymal cells that can be transported to other organs via the blood. The SNAI family, a transcriptional regulators triggers EMT, and SNAI expression is used is an indicator of malignancy. Furthermore, EMT has been suggested to be involved in drug resistance, since drug excretion from cancer cells is promoted during EMT. We showed recently that ERM proteins are induced by a member of the SNAI family, Snail. Here, we first review recent progress in research on the relationship between efflux transporters and scaffold proteins, including the question of tissue specificity. In the second part, we review the relationship between ERM scaffold proteins and the transcriptional regulatory factors that induce their expression.
Effects of the Minimal Extrathyroidal Extension on Early Response Rates after (Adjuvant) Initial Radioactive Iodine Therapy in PTC Patients
Authors: Ahmaddy Freba , Wenter Vera , Ilhan Harun , Wacker Daniel , Unterrainer Marcus , Knösel Thomas , Bartenstein Peter , Spitzweg Christine , Lehner Sebastian , Todica Andrei
Published: November 2020

Background: Extrathyroidal extension of differentiated thyroid cancer is a poor outcome factor but seems to be less significant in minimal extrathyroidal extension (mETE). However, the impact of mETE on response rate after (adjuvant) initial radioactive iodine (RAI) therapy remains unclear. We therefore compared response rates of patients with classical and follicular variants of papillary thyroid cancer (PTC) according to the updated eighth tumor-node-metastasis (TNM) classification to a control group. Methods: 455 patients with T3 (primary tumor > 4 cm) PTC according to the seventh classification who underwent total thyroidectomy followed by RAI therapy were screened. Patients formerly classified as T3 PTC solely due to mETE were reclassified into patients with T1 (primary tumor ≤ 2 cm) or T2 (primary tumor > 2 cm but ≤ 4 cm) +mETE and compared to a control group of T1/T2 −mETE PTC patients. Results: 138/455 patients were reclassified as T1/2 +mETE and compared to 317/455 T1/T2 −mETE control patients. At initial presentation, +mETE patients showed significantly higher rates of cervical lymph node metastases (p-value 0.001). Response rates were comparable in both groups (p-value n.s.). N1a/N1b-stage (Hazard ratio, HR 0.716; 95% CI 0.536–0.956, p-value 0.024) was identified as an independent prognostic factor for lower response rates. Conclusion: Response rates after RAI therapy were comparable in PTC patients irrespective of mETE but with higher rates of lymph node metastases.
Efficacy and Toxicity of Different Chemotherapy Protocols for Concurrent Chemoradiation in Non-Small Cell Lung Cancer—A Secondary Analysis of the PET Plan Trial
Authors: Gkika Eleni , Lenz Stefan , Schimek-Jasch Tanja , Waller Cornelius F. , Kremp Stephanie , Schaefer-Schuler Andrea , Mix Michael , Küsters Andreas , Tosch Marco , Hehr Thomas , Eschmann Susanne Martina , Bultel Yves-Pierre , Hass Peter , Fleckenstein Jochen , Thieme Alexander Henry , Stockinger Marcus , Dieckmann Karin , Miederer Matthias , Holl Gabriele , Rischke Hans Christian , Adebahr Sonja , König Jochem , Binder Harald , Grosu Anca-Ligia , Nestle Ursula
Published: November 2020

(1) Background: The optimal chemotherapy (CHT) regimen for concurrent chemoradiation (cCRT) is not well defined. In this secondary analysis of the international randomized PET-Plan trial, we evaluate the efficacy of different CHT. (2) Methods: Patients with inoperable NSCLC were randomized at a 1:1 ratio regarding the target volume definition and received isotoxically dose-escalated cCRT using cisplatin 80 mg/m2 (day 1, 22) and vinorelbin 15 mg/m2 (day 1, 8, 22, 29) (P1) or cisplatin 20 mg/m2 (day 1–5, 29–33) and vinorelbin 12.5 mg/m2 (day 1, 8, 15, 29, 36, 43) (P2) or carboplatin AUC1 (day 1–5, 29–33) and vinorelbin 12.5 mg/m2 (day 1, 8, 15, 29, 36, 43) (P3) or other CHT at the treating physician’s discretion. (3) Results: Between 05/2009 and 11/2016, 205 patients were randomized and 172 included in the per-protocol analysis. Patients treated in P1 or P2 had a better overall survival (OS) compared to P3 (p = 0.015, p = 0.01, respectively). Patients treated with carboplatin had a worse OS compared to cisplatin (HR 1.78, p = 0.03), but the difference did not remain significant after adjusting for age, ECOG, cardiac function creatinine and completeness of CHT. (4) Conclusions: Carboplatin doublets show no significant difference compared to cisplatin, after adjusting for possibly relevant factors, probably due to existing selection bias.
Zika Virus and Host Interactions: From the Bench to the Bedside and Beyond
Authors: Limonta Daniel , Hobman Tom C.
Published: November 2020

Before the emergence of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), the causative agent of the current COVID-19 (coronavirus disease 2019) pandemic [...]
Tumor Necrosis Factor Receptor-1 (p55) Deficiency Attenuates Tumor Growth and Intratumoral Angiogenesis and Stimulates CD8+ T Cell Function in Melanoma
Authors: Rodriguez Yamila I. , Campos Ludmila E. , Castro Melina G. , Bannoud Nadia , Blidner Ada G. , Filippa Verónica P. , Croci Diego O. , Rabinovich Gabriel A. , Alvarez Sergio E.
Published: November 2020

The role of tumor necrosis factor-α (TNF-α) in shaping the tumor microenvironment is ambiguous. Consistent with its uncertain role in melanoma, TNF-α plays a dual role, either acting as a cytotoxic cytokine or favoring a tumorigenic inflammatory microenvironment. TNF-α signals via two cognate receptors, namely TNFR1 (p55) and TNFR2 (p75), which mediate divergent biological activities. Here, we analyzed the impact of TNFR1 deficiency in tumor progression in the B16.F1 melanoma model. Tumors developed in mice lacking TNFR1 (TNFR1 knock-out; KO) were smaller and displayed lower proliferation compared to their wild type (WT) counterpart. Moreover, TNFR1 KO mice showed reduced tumor angiogenesis. Although no evidence of spontaneous metastases was observed, conditioned media obtained from TNFR1 KO tumors increased tumor cell migration. Whereas the analysis of tumor-associated immune cell infiltrates showed similar frequency of total and M2-polarized tumor-associated macrophages (TAMs), the percentage of CD8+ T cells was augmented in TNFR1 KO tumors. Indeed, functional ex vivo assays demonstrated that CD8+ T cells obtained from TNFR1KO mice displayed an increased cytotoxic function. Thus, lack of TNFR1 attenuates melanoma growth by modulating tumor cell proliferation, migration, angiogenesis and CD8+ T cell accumulation and activation, suggesting that interruption of TNF-TNFR1 signaling may contribute to control tumor burden.
The Multifaceted Output of c-Jun Biological Activity: Focus at the Junction of CD8 T Cell Activation and Exhaustion
Authors: Papavassiliou Athanasios G. , Musti Anna Maria
Published: November 2020

c-Jun is a major component of the dimeric transcription factor activator protein-1 (AP-1), a paradigm for transcriptional response to extracellular signaling, whose components are basic-Leucine Zipper (bZIP) transcription factors of the Jun, Fos, activating transcription factor (ATF), ATF-like (BATF) and Jun dimerization protein 2 (JDP2) gene families. Extracellular signals regulate c-Jun/AP-1 activity at multiple levels, including transcriptional and posttranscriptional regulation of c-Jun expression and transactivity, in turn, establishing the magnitude and the duration of c-Jun/AP-1 activation. Another important level of c-Jun/AP-1 regulation is due to the capability of Jun family members to bind DNA as a heterodimer with every other member of the AP-1 family, and to interact with other classes of transcription factors, thereby acquiring the potential to integrate diverse extrinsic and intrinsic signals into combinatorial regulation of gene expression. Here, we review how these features of c-Jun/AP-1 regulation underlie the multifaceted output of c-Jun biological activity, eliciting quite distinct cellular responses, such as neoplastic transformation, differentiation and apoptosis, in different cell types. In particular, we focus on the current understanding of the role of c-Jun/AP-1 in the response of CD8 T cells to acute infection and cancer. We highlight the transcriptional and epigenetic regulatory mechanisms through which c-Jun/AP-1 participates in the productive immune response of CD8 T cells, and how its downregulation may contribute to the dysfunctional state of tumor infiltrating CD8 T cells. Additionally, we discuss recent insights pointing at c-Jun as a suitable target for immunotherapy-based combination approaches to reinvigorate anti-tumor immune functions.
Targeting AnxA1/Formyl Peptide Receptor 2 Pathway Affords Protection against Pathological Thrombo-Inflammation
Authors: Vital Shantel A. , Senchenkova Elena Y. , Ansari Junaid , Gavins Felicity N. E.
Published: November 2020

Stroke is a leading cause of death and disability globally and is associated with a number of co-morbidities including sepsis and sickle cell disease (SCD). Despite thrombo-inflammation underlying these co-morbidities, its pathogenesis remains complicated and drug discovery programs aimed at reducing and resolving the detrimental effects remain a major therapeutic challenge. The objective of this study was to assess whether the anti-inflammatory pro-resolving protein Annexin A1 (AnxA1) was able to reduce inflammation-induced thrombosis and suppress platelet activation and thrombus formation in the cerebral microvasculature. Using two distinct models of pathological thrombo-inflammation (lipopolysaccharide (LPS) and sickle transgenic mice (STM)), thrombosis was induced in the murine brain using photoactivation (light/dye) coupled with intravital microscopy. The heightened inflammation-induced microvascular thrombosis present in these two distinct thrombo-inflammatory models was inhibited significantly by the administration of AnxA1 mimetic peptide AnxA1Ac2-26 (an effect more pronounced in the SCD model vs. the endotoxin model) and mediated by the key resolution receptor, Fpr2/ALX. Furthermore, AnxA1Ac2-26 treatment was able to hamper platelet aggregation by reducing platelet stimulation and aggregation (by moderating αIIbβ3 and P-selectin). These findings suggest that targeting the AnxA1/Fpr2/ALX pathway represents an attractive novel treatment strategy for resolving thrombo-inflammation, counteracting e.g., stroke in high-risk patient cohorts.
Quantum Chemical and Monte Carlo Simulation Studies on Inhibition Performance of Caffeine and Its Derivatives against Corrosion of Copper
Authors: Hadisaputra Saprizal , Purwoko Agus Abhi , Savalas Lalu Rudyat Telly , Prasetyo Niko , Yuanita Emmy , Hamdiani Saprini
Published: November 2020

Performance tests on caffeine’s corrosion inhibition properties and their derivatives against copper corrosion have been previously reported experimentally using gravimetric and electrochemical analyses. The test was able to measure the efficiency of their corrosion inhibition accurately. However, the caffeine and its derivatives’ structure patterns and coating mechanisms when interacting with metals during copper corrosion inhibition have not been explained in detail by experimental studies. In the present study, the theoretical density functional study (DFT), ab initio MP2, and Monte Carlo simulation approaches explain the problem. The geometrical and quantum chemical parameters of inhibitors were compared under normal and protonated conditions in the gas and aqueous environments. Theoretical studies can accurately determine the molecule’s geometrical parameters and successfully explain the quantum parameters of inhibitors. Molecular dynamics are applied to study the mechanism of interaction between inhibitors and metal surfaces in an explicit water molecule environment. The energy absorption of caffeine and its derivatives on metal surfaces was linear, with quantum parameters calculated from the density functional theory and an ab initio approach. Furthermore, these theoretical study results align with the previously reported experimental studies published by de Souza et al. The inhibition efficiency ranking of studied molecules preventing copper corrosion was caffeine > theobromine > theophylline. This theoretical approach is expected to bridge the gap in designing effective corrosion inhibitors.
Impact of Adsorption Layer Properties on Drainage Behavior of Microscopic Foam Films: The Case of Cationic/Nonionic Surfactant Mixtures
Authors: Arabadzhieva Dimi , Tchoukov Plamen , Mileva Elena
Published: November 2020

Aqueous mixtures of cationic hexadecyltrimethylammonium chloride (CTAC) and nonionic pentaethyleneglycol monododecyl ether (C12E5) are investigated. Adsorption layer properties are systematically studied within a wide concentration range for a 1:1 molar ratio of the surfactants. Surface tension and dilatational rheology measurements are conducted by profile analysis tensiometry. The interfacial data are juxtaposed to drainage kinetics and stability results for microscopic foam films, investigated by microinterferometric thin liquid film instrumentation. The obtained results give experimental evidence of synergistic interactions in the studied solutions, as compared to the corresponding single surfactant systems. Specific runs of dynamic and equilibrium surface tension curves are registered against the total surfactant quantity; the surface dilatational elasticities for the mixtures are systematically higher. A clear correlation is established between adsorption layer performance and foam film characteristics. The maxima of the film lifetimes are well outlined, and the respective values are shifted towards lower overall concentrations. The reported results substantiate the key role of the adsorption layers, and the surface dilatational properties in particular, for foam film drainage kinetics and stability. The well-expressed synergy observed in adsorption layer and foam film properties suggests the substantial benefits of using mixed surfactant systems in the design and fine-tuning of foam systems for innovative applications.
The Pseudopotential Approach within Density-Functional Theory: The Case of Atomic Metallic Hydrogen
Authors: Zhang Jin , Attapattu Jeevake , McMahon Jeffrey M.
Published: November 2020

Internal energies, enthalpies, phonon dispersion curves, and superconductivity of atomic metallic hydrogen are calculated. The standard use of pseudopotentials in density-functional theory are compared with full Coulomb-potential, all-electron linear muffin-tin orbital calculations. Quantitatively similar results are found as far as internal energies are concerned. Larger differences are found for phase-transition pressures; significant enough to affect the phase diagram. Electron–phonon spectral functions α2F(ω) also show significant differences. Against expectation, the estimated superconducting critical-temperature Tc of the first atomic metallic phase I41/amd (Cs-IV) at 500 GPa is actually higher.
Transformation of American Chestnut (Castanea dentata (Marsh.) Borkh) Using RITA® Temporary Immersion Bioreactors and We Vitro Containers
Authors: McGuigan Linda , Fernandes Patrícia , Oakes Allison , Stewart Kristen , Powell William
Published: November 2020

American chestnut (Castanea dentata (Marsh.) Borkh) was almost completely wiped out by the fungal pathogen, Cryphonectria parasitica (Murrill) M.E. Barr. Another invasive pathogen, Phytophthora cinnamomi Rands, is devastating American chestnuts in the southern region of the United States. An alternative approach for controlling these pathogens is to use genetic engineering or gene editing. We successfully transformed American chestnut with a detoxifying enzyme, oxalate oxidase, to enhance blight tolerance and more recently with the Cast_Gnk2-like gene, which encodes for an antifungal protein, to be tested for P. cinnamomi putative tolerance. Eight somatic embryo lines were transformed using three methods of selection: semisolid medium in Petri plates, liquid medium in RITA® temporary immersion bioreactors, or liquid medium in We Vitro containers. No significant differences were found between the treatments. These methods will allow for further testing of transgenes and the development of enhanced pathogen resistance in chestnut. It can serve as a model for other tree species threatened by invasive pests and pathogens.
Recovery of Phenolic Compounds from Red Grape Pomace Extract through Nanofiltration Membranes
Authors: Arboleda Mejia Jaime A. , Ricci Arianna , Figueiredo Ana S. , Versari Andrea , Cassano Alfredo , Parpinello Giuseppina P. , De Pinho Maria N.
Published: November 2020

The winemaking process generates a large amount of residues such as vine shots, stalks, grape pomace, and wine lees, which were only recently considered for exploitation of their valuable compounds. The purpose of this work was to investigate the performance of nanofiltration for the recovery of phenolic compounds, with bioactive capacity like antioxidant, from red grape pomace extract. Four membranes were compared in this study—three cellulose acetate (CA series: lab-prepared by phase inversion) and one commercial (NF90). All membranes were characterized for their hydraulic permeability and rejection coefficients to reference solutes like saccharose, glucose, raffinose, polyethylene glycol, sodium chloride, and sodium sulfate. Permeation flowrates and rejection coefficients towards total phenolics content, antioxidant activity, proanthocyanidins, glucose and fructose were measured in the nanofiltration of grape pomace extract using selected operating conditions. Among the investigated membranes, the CA400-22 exhibited the highest permeate flux (50.58 L/m2 h at 20 bar and 25 °C), low fouling index (of about 23%), the lowest rejection coefficients towards the reference solutes and the best performance in terms of separation between sugars and phenolic compounds. Indeed, the observed rejections for glucose and fructose were 19% and 12%, respectively. On the other hand, total phenolics content and proanthocyanidins were rejected for 73% and 92%, respectively.
Social Capital Contributions to Food Security: A Comprehensive Literature Review
Authors: Nosratabadi Saeed , Khazami Nesrine , Abdallah Marwa Ben , Lackner Zoltan , S. Band Shahab , Mosavi Amir , Mako Csaba
Published: November 2020

Social capital creates a synergy that benefits all members of a community. This review examines how social capital contributes to the food security of communities. A systematic literature review, based on Prisma, is designed to provide a state of the art review on capacity social capital in this realm. The output of this method led to finding 39 related articles. Studying these articles illustrates that social capital improves food security through two mechanisms of knowledge sharing and product sharing (i.e., sharing food products). It reveals that social capital through improving the food security pillars (i.e., food availability, food accessibility, food utilization, and food system stability) affects food security. In other words, the interaction among the community members results in sharing food products and information among community members, which facilitates food availability and access to food. There are many shreds of evidence in the literature that sharing food and food products among the community member decreases household food security and provides healthy nutrition to vulnerable families, and improves the food utilization pillar of food security. It is also disclosed that belonging to the social networks increases the community members’ resilience and decreases the community’s vulnerability that subsequently strengthens the stability of a food system. This study contributes to the common literature on food security and social capital by providing a conceptual model based on the literature. In addition to researchers, policymakers can use this study’s findings to provide solutions to address food insecurity problems.
Iodine and Mercury Content in Raw, Boiled, Pan-Fried, and Oven-Baked Atlantic Cod (Gadus morhua)
Authors: Dahl Lisbeth , Duinker Arne , Næss Synnøve , Markhus Maria Wik , Nerhus Ive , Midtbø Lisa Kolden , Kjellevold Marian
Published: November 2020

There is a lack of scientific evidence regarding the stability of iodine and mercury during cooking and processing of seafood. In this study, the iodine and mercury content were determined after thawing frozen fillets of Atlantic cod (Cadus morhua), and further in raw compared to boiled, pan-fried, and oven baked fillets. Iodine was determined by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) and mercury by atomic absorption spectrophotometry with Direct Mercury Analyzer (DMA-80). Thawing of the cod resulted on average in a 12% loss of iodine to the thawing water. Boiling significantly decreased the total content of iodine per slice of cod fillet corresponding to the concentration of iodine found in the boiling water. Pan-frying and oven-baking did not cause any significant changes of the total iodine content per slice of cod fillet, although iodine content per 100 g increased due to weight reduction of the cod slices from evaporation of water during preparation. For mercury, we found minimal changes of the different cooking methods. In summary, the findings in our study show that boiling had the greatest effect on the iodine content in the cod fillets. Type of cooking method should be specified in food composition databases as this in turn may influence estimation of iodine intake.
The Benefits of Plant Extracts for Human Health
Authors: Proestos Charalampos
Published: November 2020

Nature has always been, and still is, a source of foods and ingredients that are beneficial to human health [...]
Characteristics of Sunsik, a Cereal-Based Ready-to-Drink Korean Beverage, with Added Germinated Wheat and Herbal Plant Extract
Authors: Kim Bo , Park Seung , Youn Geum-Joung , Kwak Yeon , Kim Mi
Published: November 2020

The purpose of this study was to develop a formulation of Sunsik with improved health benefits by adding germinated wheat (GW) and herbal plant extract (HPE) using a response surface methodology (RSM). The central composite experimental design (CCD) was used to evaluate the effects of Sunsik with added HPE (2–4%) and GW (10–20%) on total phenolic content (TPC), total flavonoid content (TFC), Trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, gamma butyric acid (GABA) content, total color changes (△E), browning index (BI), water absorption index (WAI), and water solubility index (WSI). As a result of the CCD, the independent and dependent variables were fitted by the second-order polynomial equation, and the lack of fit for response surface models was not significant except in relation to WSI. The GABA content, TPC, and TEAC were more adequate for a linear model than for a quadratic model, and they might be affected by GW rather than HPE. Alternatively, the TFC, DPPH radical scavenging capacity, WAI, WSI, △E, and BI were fitted with quadratic models. The optimum formulation that could improve antioxidant and physicochemical properties was Sunsik with 3.5% and 20% added HPE and GW, respectively.
Multiple Sclerosis: Shall We Target CD33?
Authors: Siokas Vasileios , Tsouris Zisis , Aloizou Athina-Maria , Bakirtzis Christos , Liampas Ioannis , Koutsis Georgios , Anagnostouli Maria , Bogdanos Dimitrios P. , Grigoriadis Nikolaos , Hadjigeorgiou Georgios M. , Dardiotis Efthimios
Published: November 2020

Background: Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS). Myeloid lineage cells (microglia and macrophages) may participate in the pathogenic mechanisms leading to MS. CD33 is a transmembrane receptor, mainly expressed by myeloid lineage cells. CD33 rs3865444 is a promoter variant previously associated with Alzheimer’s disease, whose role in MS remains obscure. Objective: To assess the role of CD33 rs3865444 in MS risk. Methods: We genotyped 1396 patients with MS and 400 healthy controls for the presence of the CD33 rs3865444 variant. Odds ratios (ORs) with the respective 95% confidence intervals (CIs), were calculated with the SNPStats software, assuming five genetic models (co-dominant, dominant, recessive, over-dominant, and log-additive), with the G allele as the reference allele. The value of 0.05 was set as the threshold for statistical significance. Results: CD33 rs3865444 was associated with MS risk in the dominant (GG vs. GT + TT; OR (95% C.I.) = 0.79 (0.63–0.99), p = 0.041) and the over-dominant (GG + TT vs. GT; OR (95% C.I.) = 0.77 (0.61–0.97), p = 0.03) modes of inheritance. Given that the GG genotype was more frequent and the GT genotype was less frequent in MS patients compared to controls—while the observed frequency of the TT genotype did not differ between the two groups—the observed difference in MS risk may be stemming from either the GG (as a risk factor) or the GT (as a protective factor) genotype of CD33 rs3865444. Conclusions: Our preliminary results suggest a possible contribution of CD33 rs3865444 to MS. Therefore, larger multiethnic studies should be conducted, investigating the role of CD33 rs3865444 in MS.
Assessing the Robustness of a Factory Amid the COVID-19 Pandemic: A Fuzzy Collaborative Intelligence Approach
Authors: Chen Toly , Wang Yu-Cheng , Chiu Min-Chi
Published: November 2020

The COVID-19 pandemic has affected the operations of factories worldwide. However, the impact of the COVID-19 pandemic on different factories is not the same. In other words, the robustness of factories to the COVID-19 pandemic varies. To explore this topic, this study proposes a fuzzy collaborative intelligence approach to assess the robustness of a factory to the COVID-19 pandemic. In the proposed methodology, first, a number of experts apply a fuzzy collaborative intelligence approach to jointly evaluate the relative priorities of factors that affect the robustness of a factory to the COVID-19 pandemic. Subsequently, based on the evaluated relative priorities, a fuzzy weighted average method is applied to assess the robustness of a factory to the COVID-19 pandemic. The assessment result can be compared with that of another factory using a fuzzy technique for order preference by similarity to ideal solution. The proposed methodology has been applied to assess the robustness of a wafer fabrication factory in Taiwan to the COVID-19 pandemic.
Spring Freeze Damage of Pecan Bloom: A Review
Authors: Kaur Amandeep , Ferguson Louise , Maness Niels , Carroll Becky , Reid William , Zhang Lu
Published: November 2020

Pecan is native to the United States. The US is the world’s largest pecan producer with an average yearly production of 250 to 300 million pounds; 80 percent of the world’s supply. Georgia, New Mexico, Texas, Arizona, Oklahoma, California, Louisiana, and Florida are the major US pecan producing states. Pecan trees frequently suffer from spring freeze at bud break and bloom as the buds are quite sensitive to freeze damage. This leads to poor flower and nut production. This review focuses on the impact of spring freeze during bud differentiation and flower development. Spring freeze kills the primary terminal buds, the pecan tree has a second chance for growth and flowering through secondary buds. Unfortunately, secondary buds have less bloom potential than primary buds and nut yield is reduced. Spring freeze damage depends on severity of the freeze, bud growth stage, cultivar type and tree age, tree height and tree vigor. This review discusses the impact of temperature on structure and function of male and female reproductive organs. It also summarizes carbohydrate relations as another factor that may play an important role in spring growth and transition of primary and secondary buds to flowers.
Study on the Effect of Streets’ Space Forms on Campus Microclimate in the Severe Cold Region of China—Case Study of a University Campus in Daqing City
Authors: Jin Hong , Qiao Liang , Cui Peng
Published: November 2020

In urban areas, local microclimate is influenced by architectural forms, which will in turn affect human comfort. Taking Daqing as an example, this article studies the microclimate of a university campus in the severe cold area in China. Based on the space features of the streets, we categorize the streets into three types: open type, semi-open type, and street-entry type. Through analysis, this article researches microclimates of the three kinds of streets, the influence of building heating on the surrounding thermal environment, the relationship between streets’ morphology features and microclimate and human comfort (physiological equivalent temperature, PET). By study and analysis, we have the following findings: for open-type streets, the average globe temperatures of streets with different orientations can reach 1.3 °C in winter because of the influence of sidewalk trees. For semi-open-type streets, streets temperature is under the influence of the locating directions of buildings. The maximum air temperature difference among streets with different building arrangements reaches 2.1 °C in winter. For street-entry-type streets, the height–width ratios and orientations of streets are related to the continuity degree of the street interfaces. The building interface acts as a heating element and affect the surrounding thermal environment by heat convection and heat radiation. Analysis demonstrates that heat convection has a more obvious effect on rising surrounding temperature than heat radiation. Buildings with higher heat radiation witness higher globe temperature. For street-entry-type streets and semi-open-type streets, the SVF (sky view factor) and L/C (plane opening rate) of streets are negatively correlated with temperature and PET, but positively correlated with wind speed. If the SVF increases 0.1, the air temperature will reduce 0.1 °C, the wind speed will increase 0.19 m/s, and the PET will reduce 0.7 °C.
Air Pollution-Related Health Impacts on Individuals Experiencing Homelessness: Environmental Justice and Health Vulnerability in Salt Lake County, Utah
Authors: DeMarco Angelina L. , Hardenbrook Rebecca , Rose Jeff , Mendoza Daniel L.
Published: November 2020

Experiences of homelessness, although widely varied, are characterized by extensive time in public spaces, often outdoors. However, there has been little empirical research about the ways in which environmental factors affect individuals experiencing homelessness (IEHs). Therefore, the purpose of this study was to use an environmental justice approach to understand how cardiopulmonary health of IEHs is affected by episodic poor air quality in Salt Lake County. It was hypothesized that people who had experienced unsheltered homelessness and those who had been experiencing homelessness for longer periods of time would report greater health difficulties from poor air quality exposure. Through a combination of in-person semistructured interviews with IEHs (n = 138) and access to corresponding state-based service provider databases, researchers examined both overall descriptives of and relationships between types (sheltered and unsheltered) and duration (chronic and nonchronic) of homelessness. More than 61% of IEHs reported physical reactions to air pollution, 37% reported air pollution-related emotional stress, and more than 89% had sought medical attention for a condition related to air pollution. Findings indicate that while IEHs report a number of health effects related to poor air quality, there were no significant differences between individuals based on either sheltered status or duration of their experiences of homelessness. This study provides an initial empirical inquiry to understand how environmental disamenities negatively influence IEHs, as well as noting that sheltered status and duration of homelessness are less impactful than originally hypothesized.
Exploring Daily Activity Patterns on the Typical Day of Older Adults for Supporting Aging-in-Place in China’s Rural Environment
Authors: Zhang Ziqi , Qiu Zhi
Published: November 2020

Severe aging in rural China is prompting communities to promote support for older people to age in place. The study of the daily life of older adults in rural areas is conducive to understanding their real life and demands, as well as the way they interact with their environment, to develop feasible strategies. In this study, 171 older adults over 60 years old in two different types of villages in Northern Zhejiang Province were investigated and analyzed in terms of the temporal and spatial features of daily activities, as well as their relationship with population attributes, personal competence, and subjective demands. The results show that: (1) significant association can be seen between working hours and the demand for health services, housework hours and gender and age, as well as leisure hours and ADL and the demand for recreational services. (2) The older adults appear to have inter-group homogeneity in some aspects: basic living activities, leisure hours, the gender difference in housework hours, and recreational preference, while they have higher average paid work hours and fewer leisure alternatives than their urban counterparts. Their definitions of paid work, housework, and leisure activities are vague. (3) The definition of home by the older adults in rural places sometimes seems to go beyond the scope of their own house, and the extensive definition of home may change their recognitions of some activities. They also inclined to assign meaning to a place through frequent use rather than through external definitions. (4) The weak consciousness on buying services and deteriorated financial situation hinders the older adults in rural communities from expressing their real demands. Unspoken demands include economic security, recreational choices, and assistance in housework. The results will help to provide references for the improvement of eldercare services and the community environment.
Impact of Endotoxins in Gelatine Hydrogels on Chondrogenic Differentiation and Inflammatory Cytokine Secretion In Vitro
Authors: Groen Wilhelmina M. G. A. C. , Utomo Lizette , Castilho Miguel , Gawlitta Debby , Malda Jos , Weeren P. René van , Levato Riccardo , Korthagen Nicoline M.
Published: November 2020

Gelatine methacryloyl (GelMA) hydrogels are widely used in studies aimed at cartilage regeneration. However, the endotoxin content of commercially available GelMAs and gelatines used in these studies is often overlooked, even though endotoxins may influence several cellular functions. Moreover, regulations for clinical use of biomaterials dictate a stringent endotoxin limit. We determined the endotoxin level of five different GelMAs and evaluated the effect on the chondrogenic differentiation of equine mesenchymal stromal cells (MSCs). Cartilage-like matrix production was evaluated by biochemical assays and immunohistochemistry. Furthermore, equine peripheral blood mononuclear cells (PBMCs) were cultured on the hydrogels for 24 h, followed by the assessment of tumour necrosis factor (TNF)-α and C–C motif chemokine ligand (CCL)2 as inflammatory markers. The GelMAs were found to have widely varying endotoxin content (two with >1000 EU/mL and three with <10 EU/mL), however, this was not a critical factor determining in vitro cartilage-like matrix production of embedded MSCs. PBMCs did produce significantly higher TNF-α and CCL2 in response to the GelMA with the highest endotoxin level compared to the other GelMAs. Although limited effects on chondrogenic differentiation were found in this study, caution with the use of commercial hydrogels is warranted in the translation from in vitro to in vivo studies because of regulatory constraints and potential inflammatory effects of the content of these hydrogels.
Evaluation of Pollutant Emissions into the Atmosphere during the Loading of Hydrocarbons in Marine Oil Tankers in the Arctic Region
Authors: Fetisov Vadim , Pshenin Vladimir , Nagornov Dmitrii , Lykov Yuri , Mohammadi Amir H.
Published: November 2020

Emissions of volatile organic compounds into the atmosphere when loading oil or petroleum products into tankers are strong environmental pollutants. Given the increase in oil transport by sea and the development of Arctic routes, humanity faces the task of preserving the Arctic ecosystem. Vapor recovery units can limit the emissions of volatile organic compounds. However, it is necessary to estimate the emissions of oil and petroleum products vapors. This article offers two methods for estimating emissions of volatile organic compounds. In the analytical method, a mathematical model of evaporation dynamics and forecasting tank gas space pressure of the tanker is proposed. The model makes it possible to estimate the throughput capacity of existing gas phase discharge pipeline systems and is also suitable for designing new oil vapor recovery units. Creating an experimental laboratory stand is proposed in the experimental method, and its possible technological scheme is developed.
Galactomannan Produced by Aspergillus fumigatus: An Update on the Structure, Biosynthesis and Biological Functions of an Emblematic Fungal Biomarker
Authors: Fontaine Thierry , Latgé Jean-Paul
Published: November 2020

The galactomannan (GM) that is produced by the human fungal pathogen Aspergillus fumigatus is an emblematic biomarker in medical mycology. The GM is composed of two monosaccharides: mannose and galactofuranose. The furanic configuration of galactose residues, absent in mammals, is responsible for the antigenicity of the GM and has favoured the development of ELISA tests to diagnose aspergillosis in immunocompromised patients. The GM that is produced by A. fumigatus is a unique fungal polysaccharide containing a tetramannoside repeat unit and having three different forms: (i) membrane bound through a glycosylphosphatidylinositol (GPI)-anchor, (ii) covalently linked to β-1,3-glucans in the cell wall, or (iii) released in the culture medium as a free polymer. Recent studies have revealed the crucial role of the GM during vegetative and polarized fungal growth. This review highlights these recent data on its biosynthetic pathway and its biological functions during the saprophytic and pathogenic life of this opportunistic human fungal pathogen.
The Fission Yeast RNA-Binding Protein Meu5 Is Involved in Outer Forespore Membrane Breakdown during Spore Formation
Authors: Zhang Bowen , Teraguchi Erika , Imada Kazuki , Tahara Yuhei O. , Nakamura Shuko , Miyata Makoto , Kagiwada Satoshi , Nakamura Taro
Published: November 2020

In Schizosaccharomyces pombe, the spore wall confers strong resistance against external stress. During meiosis II, the double-layered intracellular forespore membrane (FSM) forms de novo and encapsulates the nucleus. Eventually, the inner FSM layer becomes the plasma membrane of the spore, while the outer layer breaks down. However, the molecular mechanism and biological significance of this membrane breakdown remain unknown. Here, by genetic investigation of an S. pombe mutant (E22) with normal prespore formation but abnormal spores, we showed that Meu5, an RNA-binding protein known to bind to and stabilize more than 80 transcripts, is involved in this process. We confirmed that the E22 mutant does not produce Meu5 protein, while overexpression of meu5+ in E22 restores the sporulation defect. Furthermore, electron microscopy revealed that the outer membrane of the FSM persisted in meu5∆ spores. Investigation of the target genes of meu5+ showed that a mutant of cyc1+ encoding cytochrome c also showed a severe defect in outer FSM breakdown. Lastly, we determined that outer FSM breakdown occurs coincident with or after formation of the outermost Isp3 layer of the spore wall. Collectively, our data provide novel insights into the molecular mechanism of spore formation.
Overexpression of the Aspergillus fumigatus Small GTPase, RsrA, Promotes Polarity Establishment during Germination
Authors: Martin-Vicente Adela , Souza Ana C. O. , Nywening Ashley V. , Ge Wenbo , Fortwendel Jarrod R.
Published: November 2020

Cell polarization comprises highly controlled processes and occurs in most eukaryotic organisms. In yeast, the processes of budding, mating and filamentation require coordinated mechanisms leading to polarized growth. Filamentous fungi, such as Aspergillus fumigatus, are an extreme example of cell polarization, essential for both vegetative and pathogenic growth. A major regulator of polarized growth in yeast is the small GTPase Rsr1, which is essential for bud-site selection. Here, we show that deletion of the putative A. fumigatus ortholog, rsrA, causes only a modest reduction of growth rate and delay in germ tube emergence. In contrast, overexpression of rsrA results in a morphogenesis defect, characterized by a significant delay in polarity establishment followed by the establishment of multiple growth axes. This aberrant phenotype is reversed when rsrA expression levels are decreased, suggesting that correct regulation of RsrA activity is crucial for accurate patterning of polarity establishment. Despite this finding, deletion or overexpression of rsrA resulted in no changes of A. fumigatus virulence attributes in a mouse model of invasive aspergillosis. Additional mutational analyses revealed that RsrA cooperates genetically with the small GTPase, RasA, to support A. fumigatus viability.
Swedish Government and Country Image during the International Media Coverage of the Coronavirus Pandemic Strategy: From Bold to Pariah
Authors: Simons Greg
Published: November 2020

The Coronavirus pandemic has caused a great deal of disruption and fear for countries and peoples around the globe. It has spread in a seemingly unstoppable manner, which has created a global crisis in terms of strategies to mitigate its effects, and how these strategies are communicated is vital to the credibility and legitimacy of those relevant actors. This paper examines and analyses how the Swedish government attempted to frame its pandemic strategy in the international mass media as a successful example of the ‘Swedish model’. Initially, this was successful and those frames seemed to be accepted; however, this soon changed and instead of serving as an example to emulate it became a model to avoid. Using hegemony theory and indexing theory, this paper attempts to explain how and why this change occurred. The change in the coverage becomes apparent as the Swedish government’s coronavirus policy and strategy of Sweden begins to increasingly diverge from the global orthodoxy of other countries, including the United Kingdom. Therefore, the politics of crisis management among the political elite begin to diverge and the politics of crisis management creates a more hostile and critical environment.