bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation on Hysteretic Behavior of Embedded PVC Pipe Confined Reinforced High Strength Concrete Columns
Authors: Chen Zongping , Liang Yuhan , Zhao Xuebing , Zhou Ji
Published: February 2020

To study the seismic performance of embedded polyvinyl chloride (PVC) pipe confined reinforced high-strength concrete (PVC-RHC) columns, five specimens are designed for cyclic loading test, which include three PVC-RHC column specimens, an embedded circle steel tube confined reinforced high-strength concrete (CST-RHC) column specimen, and a reinforced high-strength concrete (RHC) column specimen. The failure mechanism and morphology are revealed by experiments. The influences of PVC pipe diameter, axial compression ratio, and concrete strength on seismic performance indexes are analyzed. The research results indicate thhe following: all specimens displayed shear baroclinic failure. Compared with RHC specimens, the hysteretic curves of the PVC-RHC specimen and CST-RHC specimen were fuller; furthermore, their energy dissipation capacity, deformation, and ductility were more beneficial. With the increase of the diameter–length ratio and axial pressure, the energy dissipation capacity and deformation capacity of PVC-RHC specimens decreased. The shear bearing capacity of the PVC-RHC specimen calculated with “concrete structure design code” (GB 50010-2010) was smaller than the test results by 25%, showing an excessive safety margin. Thus, according to the failure mechanism of the PVC-RHC specimen, a new calculation formula of shear bearing capacity is deduced, which is in good agreement with the experimental results.
Electroactive γ-Phase, Enhanced Thermal and Mechanical Properties and High Ionic Conductivity Response of Poly (Vinylidene Fluoride)/Cellulose Nanocrystal Hybrid Nanocomposites
Authors: Lizundia Erlantz , Reizabal Ander , Costa Carlos M. , Maceiras Alberto , Lanceros-Méndez Senentxu
Published: February 2020

Cellulose nanocrystals (CNCs) were incorporated into poly (vinylidene fluoride) (PVDF) to tailor the mechanical and dielectric properties of this electroactive polymer. PVDF/CNC nanocomposites with concentrations up to 15 wt.% were prepared by solvent-casting followed by quick vacuum drying in order to ensure the formation of the electroactive γ-phase. The changes induced by the presence of CNCs on the morphology of PVDF and its crystalline structure, thermal properties, mechanical performance and dielectric behavior are explored. The results suggest a relevant role of the CNC surface −OH groups, which interact with PVDF fluorine atoms. The real dielectric constant ε’ of nanocomposites at 200 Hz was found to increase by 3.6 times up to 47 for the 15 wt.% CNC nanocomposite due to an enhanced ionic conductivity provided by CNCs. The approach reported here in order to boost the formation of the γ-phase of PVDF upon the incorporation of CNCs serves to further develop cellulose-based multifunctional materials.
Low Scattering Microstrip Antenna Based on Broadband Artificial Magnetic Conductor Structure
Authors: Saleem Muhammad , Li Xiao-Lai
Published: February 2020

In this summary, we have suggested a new technique in which destructive interference principle is incorporated into a chessboard like a reflective screen, and the proposed antenna realizes a remarkable in-band and also out-of-band backscattered energy reduction by using a metasurface (MS). Two different MS unit cells are designed to provide the resonant frequency with a zero-degree reflection phase. Metasurface unit cells are configured in a chessboard-like reflector screen to achieve the reflection phase difference of 180° ± 37° over a broadband range of frequencies to redirect the scattering field into four quadrants. It is implemented to reduce the backscattered energy level of the microstrip antenna, which is based on destructive interference principle. The simulations indicate that the proposed antenna possesses significant backscattered energy reduction from 6 GHz to 16 GHz in both x– and y– polarization and also −10 dB backscattering reduction at antenna working band (7.4–7.8 GHz) is covered. Moreover, the radiation performance is preserved well and artificial magnetic conductor (AMC) unit cells work at different frequencies which are not influenced on the radiation properties. The bistatic performance of the antenna at different frequencies is also presented. Measurements and simulations of the fabricated design coincide well and the proposed design is verified and validated successfully.
Effect of Sand Particle Size on Microstructure and Mechanical Properties of Gypsum-Cemented Similar Materials
Authors: Guan Weiming , Qi Qi , Zhang Zhiyi , Nan Senlin
Published: February 2020

To identify mechanism of sand particle size effect on the mechanical properties of gypsum cement, 11 grades of sand particles with a size of 0.1–3 mm were used to produce 99 specimens for uniaxial compression and permeability coefficient testing. Based on this, the distribution characteristics of internal stress and horizontal displacement are discussed using the numerical analysis. The results obtained show that the sand particle size effect on the uniaxial compressive strength of similar materials is negatively correlated within the range from −16.51% to 49.79%. SEM observations imply that, in the case of small particle sizes, gypsum crystals develop into denser needle-like structures, while for larger particle sizes, they are mostly loose lamellar structures. Permeability tests indicated that the larger the sand particle size, the greater the permeability, indicating that the internal pore connectivity is better, and the crevices are easier to penetrate when the specimen is compressed. Numerical simulations indicated that the larger the particle size, the larger the extreme deformation value of the specimen in the horizontal direction, and the more uneven the deformation distribution. In addition, specimens with larger particle sizes had a larger total area, where the tensile stress exceeded the ultimate tensile strength, and were more prone to tensile failure.
Thermal Stability and Kinetics of Formation of Magnesium Oxychloride Phase 3Mg(OH)2∙MgCl2∙8H2O
Authors: Lojka Michal , Jankovský Ondřej , Jiříčková Adéla , Lauermannová Anna-Marie , Antončík Filip , Sedmidubský David , Pavlík Zbyšek , Pavlíková Milena
Published: February 2020

In this paper, magnesium oxychloride cement with stoichiometry 3Mg(OH)2∙MgCl2∙8H2O (MOC 3-1-8) was prepared and characterized. The phase composition and kinetics of formation were studied by X-ray diffraction (XRD) and Rietveld analysis of obtained diffractograms. The chemical composition was analyzed using X-ray fluorescence (XRF) and energy dispersive spectroscopy (EDS). Furthermore, scanning electron microscopy (SEM) was used to study morphology, and Fourier Transform Infrared (FT-IR) spectroscopy was also used for the analysis of the prepared sample. In addition, thermal stability was tested using simultaneous thermal analysis (STA) combined with mass spectroscopy (MS). The obtained data gave evidence of the fast formation of MOC 3-1-8, which started to precipitate rapidly. As the length of the time of ripening increased, the amount of MgO decreased, while the amount of MOC 3-1-8 increased. The fast formation of the MOC 3-1-8 phase at an ambient temperature is important for its application in the production of low-energy construction materials, which corresponds with the challenges of a sustainable building industry.
Bioactive Surfaces of Polylactide and Silver Nanoparticles for the Prevention of Microbial Contamination
Authors: Gherasim Oana , Grumezescu Alexandru Mihai , Grumezescu Valentina , Iordache Florin , Vasile Bogdan Stefan , Holban Alina Maria
Published: February 2020

Thanks to its peculiar interactions with biological molecules and structures, metallic silver in the form of silver nanoparticles achieved a remarkable comeback as a potential antimicrobial agent. The antimicrobial use of silver nanoparticles is of clinical importance, as several pathogenic microorganisms developed resistance against various conventional drug treatments. Hence, given the extensive efficiency of silver nanoparticles against drug-sensitive and drug-resistant pathogens, their therapeutic implications were demonstrated in multiple medical applications, such as silver-based dressings, silver-coated biomedical devices and silver-containing nanogels. Bacterial strains possess an intrinsic ability to form well-organized microbial communities, capable of developing adaptive mechanisms to environmental aggression and self-protective pathways against antibiotics. The formation of these mono- or poly-microbial colonies, called biofilms, is closely related with the occurrence of infectious processes which result in severe and chronic pathologies. Therefore, substantial efforts were oriented to the development of new protective coatings for biomedical surfaces, capable of sustaining the physiological processes within human-derived normal cells and to disrupt the microbial contamination and colonization stages. Nanostructured materials based on polylactic acid and silver nanoparticles are herein proposed as bioactive coatings able to prevent the formation of microbial biofilms on biomedical relevant surfaces.
Mechanical Properties of Ultra-High Performance Concrete before and after Exposure to High Temperatures
Authors: Chen How-Ji , Yu Yi-Lin , Tang Chao-Wei
Published: February 2020

Compared with ordinary concrete, ultra-high performance concrete (UHPC) has excellent toughness and better impact resistance. Under high temperatures, the microstructure and mechanical properties of UHPC may seriously deteriorate. As such, we first explored the properties of UHPC with a designed 28-day compressive strength of 120 MPa or higher in the fresh mix phase, and measured its hardened mechanical properties at seven days. The test variables included: the type of cementing material and the mixing ratio (silica ash, ultra-fine silicon powder), the type of fiber (steel fiber, polypropylene fiber), and the fiber content (volume percentage). In addition to the UHPC of the experimental group, pure concrete was used as the control group in the experiment; no fiber or supplementary cementitious materials (silica ash, ultra-fine silicon powder) were added to enable comparison and discussion and analysis. Then, the UHPC-1 specimens of the experimental group were selected for further compressive, flexural, and splitting strength tests and SEM observations after exposure to different target temperatures in an electric furnace. The test results show that at room temperature, the 56-day compressive strength of the UHPC-1 mix was 155.8 MPa, which is higher than the >150 MPa general compressive strength requirement for ultra-high-performance concrete. The residual compressive strength, flexural strength, and splitting strength of the UHPC-1 specimen after exposure to 300, 400, and 500 °C did not decrease significantly, and even increased due to the drying effect of heating. However, when the temperature was 600 °C, spalling occurred, so the residual mechanical strength rapidly declined. SEM observations confirmed that polypropylene fibers melted at high temperatures, thereby forming other channels that helped to reduce the internal vapor pressure of the UHPC and maintain a certain residual strength.
Evaluation of Graphite Nanoplatelets Influence on the Lubrication Properties of Asphalt Binders
Authors: Yan Tianhao , Ingrassia Lorenzo Paolo , Kumar Ravi , Turos Mugurel , Canestrari Francesco , Lu Xiaohu , Marasteanu Mihai
Published: February 2020

With the major advance in nanotechnology, there has been an emerging interest in applying nanoscale materials to asphalt pavement materials. Among them, considerable interest has been directed to carbon-based nanomaterials, such as carbon nanotubes (CNTs) and graphite nanoplatelets (GNPs). Recent studies have proven that the addition of small percentages of GNPs could significantly reduce the compaction effort required to densify HMA. Viscosity measurements showed, however, that the addition of GNPs increased the viscosity of the binder. This observation pointed towards the presence of a different mechanism responsible for the reduction of compaction effort. A new test method used for lubricants and based on tribology has been recently proposed in order to characterize the lubricating behaviour of asphalt binders. In this study, the tribological characterization of an asphalt binder modified with GNPs was performed. A novel approach in which aggregate surface microtexture was simulated using rough surfaces of the testing fixtures, shows that indeed, the addition of GNPs lowers the friction coefficient and therefore, enhances the lubrication properties of the binder when mixed with mineral aggregates.
A Game Theoretic Model of Choosing a Valuable Good via a Short List Heuristic
Authors: Ramsey David M.
Published: February 2020

The Internet gives access to a huge amount of data at the click of a mouse. This is very helpful when consumers are making decisions about which product to buy. However, the final decision to purchase is still generally made by humans who have limited memory and perception. The short list heuristic is often used when there are many offers on the market. Searchers first find information about offers via the Internet and on this basis choose a relatively small number of offers to view in real life. Although such rules are often used in practice, little research has been carried out on determining, for example, what the size of the short list should be depending on the parameters of the problem or modelling how the short list heuristic can be implemented when there are multiple decision makers. This article presents a game theoretic model of such a search procedure with two players. These two players can be interpreted, for example, as a couple searching for a flat or a second-hand car. The model indicates that under such a search procedure the roles of searchers should only be divided when the preferences of the players are coherent or there is a high level of goodwill between them. In other cases, dividing the roles leads to a high level of conflict.
pth Moment Stability of a Stationary Solution for a Reaction Diffusion System with Distributed Delays
Authors: Wang Xiongrui , Rao Ruofeng , Zhong Shouming
Published: February 2020

In this paper, the Sobolev embedding theorem, Holder inequality, the Lebesgue contrl convergence theorem, the operator norm estimation technique, and critical point theory are employed to prove the existence of nontrivial stationary solution for p-Laplacian diffusion system with distributed delays. Furthermore, by giving the definition of pth moment stability, the authors use the Lyapunovfunctional method and Kamke function to derive the stability of nontrivialstationary solution. Moreover, a numerical example illuminates the effectiveness of the proposed methods. Finally, an interesting further thought is put forward, which is conducive to the in-depth study of the problem.
Aircraft Trajectory Tracking Using Radar Equipment with Fuzzy Logic Algorithm
Authors: Raboaca Maria Simona , Dumitrescu Catalin , Manta Ioana
Published: February 2020

Radio-electronic means, including equipment for transmissions, radio-location, broadcasting, and navigation, allow the execution of various research missions and combat forces management. Determining the target coordinates and directing the armament towards them, obtaining and processing data about enemies, ensuring the navigation of ships, planes and outer atmospheric means, transmitting orders, decisions, reports and other necessary information for the armed forces; these are only some of the possibilities of radio-electronic technology. Fuzzy logic allows the linguistic description of the laws of command, operation and control of a system. When working with complex and nonlinear systems, it can often be observed that, as their complexity increases, there is a decrease in the significance of the details in describing the global behavior of the system. Even though such an approach may seem inadequate, it is often superior and less laborious than a rigorous mathematical approach. The main argument in favor of fuzzy set theory is to excel in operating with imprecise, vague notions. This article demonstrates the superiority of a fuzzy tracking system over the standard Kalman filter tracking system under the conditions of uneven accelerations and sudden change of direction of the targets, as well as in the case of failure to observe the target during successive scans. A cascading Kalman filtering algorithm was used to solve the speed ambiguity and to reduce the measurement error in real-time radar processing. The cascade filters are extended Kalman filters with controlled gain using fuzzy logic for tracking targets using radar equipment under difficult tracking conditions.
Fatigue Examination of HSLA Steel with Yield Strength of 960 MPa and Its Welded Joints under Strain Mode
Authors: Ślęzak Tomasz
Published: February 2020

The full benefits of application the high strength low alloyed steels HSLA can be achieved if the structures will be able to carry the alternate loads and fatigue cracks will not be formed, even in the vicinity of welded joints. For this reason the purpose of this study is to find and to explain the influence of different factors on fatigue crack initiation and the nature of crack propagation in HSLA steel and its welded joints. The S960QL steel and two types of welded joints were subjected to low cycle fatigue (LCF) tests at a strain mode and the received surfaces of fractures were analyzed using SEM microscope. Additionally, the microhardness measurements and the residual stress analyze in a cross-section of the joint were conducted. The maximum hardness was determined on the fusion line and more favorable hardness distribution was in the square joints than in single-V. Compiled maps of residual stresses have shown that the local orientation and values of the principal stress vector near the fusion line can influence negative the fatigue life. Finally, the square joints tested in the low cycle fatigue regime have shown a slightly higher fatigue life in comparison with single-V.
Systemic Effects of Radiotherapy and Concurrent Chemo-Radiotherapy in Head and Neck Cancer Patients—Comparison of Serum Metabolome Profiles
Authors: Jelonek Karol , Krzywon Aleksandra , Jablonska Patrycja , Slominska Ewa M. , Smolenski Ryszard T. , Polanska Joanna , Rutkowski Tomasz , Mrochem-Kwarciak Jolanta , Skladowski Krzysztof , Widlak Piotr
Published: February 2020

Anticancer treatment induces systemic molecular changes that could be detected at the level of biofluids. Understanding how human metabolism is influenced by these treatments is crucial to predict the individual response and adjust personalized therapies. Here, we aimed to compare profiles of metabolites in serum of head and neck cancer patients treated with concurrent chemo-radiotherapy, radiotherapy alone, or induction chemotherapy. Serum samples were analyzed by a targeted quantitative approach using combined direct flow injection and liquid chromatography coupled to tandem mass spectrometry, which allowed simultaneous quantification of 149 metabolites. There were 45 metabolites whose levels were significantly changed between pretreatment and within- or post-treatment serum samples, including 38 phospholipids. Concurrent chemo-radiotherapy induced faster and stronger effects than radiotherapy alone. On the other hand, chemotherapy alone did not result in significant changes. The decreased level of total phospholipids was the most apparent effect observed during the first step of the treatment. This corresponded to the loss of patients’ body mass, yet no correlation between both parameters was observed for individual patients. We concluded that different molecular changes were measured at the level of serum metabolome in response to different treatment modalities.
Design and Fabrication of a Novel MEMS Relay with Low Actuation Voltage
Authors: Li Hao , Ruan Yong , You Zheng , Song Zhiqiang
Published: February 2020

Compared with conventional solid-state relays, micro-electro mechanical system (MEMS) relays have the advantages of high isolation, low contact resistance, low power consumption, and abrupt switching characteristics. Nevertheless, the widespread application of MEMS relays has been limited due to the issue of the conflict between low actuation voltages and high device performance. This article presents a novel cantilever MEMS relay with an embedded contact electrode which helps to achieve a low actuation voltage (below 8 V) and high restoring force simultaneously. Meanwhile, the contact resistance is as low as around 0.4 Ω and the reliability is verified. To thoroughly investigate and analyze the novel cantilever MEMS relay, a static theoretical model of the structure was developed. Based on the model, the cantilever MEMS relay was designed and optimized. Then, the relays were fabricated by the bulk-silicon micromachining process based on the silicon–glass anodic bonding technology. Finally, the switching performance of the novel cantilever MEMS relay was measured. Experimental results demonstrate that the proposed MEMS relay has a low actuation voltage below 8 V and high performance, which is in good agreement with the simulation results, and shows significant advantages when compared with previous reports. Therefore, the proposed MEMS relay with an embedded contact electrode is promising in practical applications.
Actuation Mechanism of Microvalves: A Review
Authors: Qian Jin-Yuan , Hou Cong-Wei , Li Xiao-Juan , Jin Zhi-Jiang
Published: February 2020

The microvalve is one of the most important components in microfluidics. With decades of development, the microvalve has been widely used in many industries such as life science, chemical engineering, chip, and so forth. This paper presents a comprehensive review of the progress made over the past years about microvalves based on different actuation mechanisms. According to driving sources, plenty of actuation mechanisms are developed and adopted in microvalves, including electricity, magnetism, gas, material and creature, surface acoustic wave, and so on. Although there are currently a variety of microvalves, problems such as leakage, low precision, poor reliability, high energy consumption, and high cost still exist. Problems deserving to be further addressed are suggested, aimed at materials, fabrication methods, controlling performances, flow characteristics, and applications.
Organocatalytic Asymmetric Aldol Reaction of Arylglyoxals and Hydroxyacetone: Enantioselective Synthesis of 2,3-Dihydroxy-1,4-diones
Authors: Zhou Yu-Hao , Zhang Yu-Zu , Wu Zhu-Lian , Cai Tian , Wen Wei , Guo Qi-Xiang
Published: February 2020

A highly efficient quinine-derived primary-amine-catalyzed asymmetric aldol addition of hydroxyacetone to arylglyoxals is described. Structurally diverse anti-2,3-dihydroxy-1,4-diones were generated in high yields, with good diastereoselectivities and enantioselectivities.
Antitumoral Properties of Natural Products
Authors: Fabiani Roberto
Published: February 2020

Cancer is one of the major causes of death worldwide [...]
Cannabinoids in the Pathophysiology of Skin Inflammation
Authors: Scheau Cristian , Badarau Ioana Anca , Mihai Livia-Gratiela , Scheau Andreea-Elena , Costache Daniel Octavian , Constantin Carolina , Calina Daniela , Caruntu Constantin , Costache Raluca Simona , Caruntu Ana
Published: February 2020

Cannabinoids are increasingly-used substances in the treatment of chronic pain, some neuropsychiatric disorders and more recently, skin disorders with an inflammatory component. However, various studies cite conflicting results concerning the cellular mechanisms involved, while others suggest that cannabinoids may even exert pro-inflammatory behaviors. This paper aims to detail and clarify the complex workings of cannabinoids in the molecular setting of the main dermatological inflammatory diseases, and their interactions with other substances with emerging applications in the treatment of these conditions. Also, the potential role of cannabinoids as antitumoral drugs is explored in relation to the inflammatory component of skin cancer. In vivo and in vitro studies that employed either phyto-, endo-, or synthetic cannabinoids were considered in this paper. Cannabinoids are regarded with growing interest as eligible drugs in the treatment of skin inflammatory conditions, with potential anticancer effects, and the readiness in monitoring of effects and the facility of topical application may contribute to the growing support of the use of these substances. Despite the promising early results, further controlled human studies are required to establish the definitive role of these products in the pathophysiology of skin inflammation and their usefulness in the clinical setting.
SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay
Authors: Putz Ana-Maria , Ianăși Cătălin , Dudás Zoltán , Coricovac Dorina , Watz Claudia (Farcas) , Len Adél , Almásy László , Sacarescu Liviu , Dehelean Cristina
Published: February 2020

A facile sol-gel route has been applied to synthesize hybrid silica-PVA-iron oxide nanocomposite materials. A step-by-step calcination (processing temperatures up to 400 °C) was applied in order to oxidize the organics together with the iron precursor. Transmission electron microscopy, X-ray diffraction, small angle neutron scattering, and nitrogen porosimetry were used to determine the temperature-induced morpho-textural modifications. In vitro cytotoxicity assay was conducted by monitoring the cell viability by the means of MTT assay to qualify the materials as MRI contrast agents or as drug carriers. Two cell lines were considered: the HaCaT (human keratinocyte cell line) and the A375 tumour cell line of human melanoma. Five concentrations of 10 µg/mL, 30 µg/mL, 50 µg/mL, 100 µg/mL, and 200 µg/mL were tested, while using DMSO (dimethylsulfoxid) and PBS (phosphate saline buffer) as solvents. The HaCaT and A375 cell lines were exposed to the prepared agent suspensions for 24 h. In the case of DMSO (dimethyl sulfoxide) suspensions, the effect on human keratinocytes migration and proliferation were also evaluated. The results indicate that only the concentrations of 100 μg/mL and 200 μg/mL of the nanocomposite in DMSO induced a slight decrease in the HaCaT cell viability. The PBS based in vitro assay showed that the nanocomposite did not present toxicity on the HaCaT cells, even at high doses (200 μg/mL agent).
Impact Mineralization of Chokeberry and Cranberry Fruit Juices Using a New Functional Additive on the Protection of Bioactive Compounds and Antioxidative Properties
Authors: Lachowicz Sabina , Oszmiański Jan , Wilczyńska Martyna , Zaguła Grzegorz , Saletnik Bogdan , Puchalski Czesław
Published: February 2020

Chicken eggshells can be used as an attractive dietary source of mineral compounds, including calcium (Ca). However, the effects of chicken eggshell powder (CESP) on berry fruit juices have not been studied to date. Therefore, the objective of this study was to evaluate the effect of its addition to juices from chokeberry and cranberry on their phytochemical properties. The juices were determined for contents of polyphenols (determined by ultra-efficient liquid chromatography coupled with a mass detector (UPLC-PDA-ESI-MS/MS)), macro- and microelements (by inductively coupled plasma - optical emission spectrometry (ICP-OES)), and organic acids (by high-performance liquid chromatography (HPLC-PDA)) as well as for their antioxidative activity by radical scavenging capacity (ABTS) and ferric reducing antioxidative power (FRAP) assay, color profile (CIE L* a* b* system), and sensory attributes. The study results demonstrate that CESP addition to chokeberry and cranberry juices enriched them with minerals and increased their Ca content 25.7 times and 66.3 times, respectively, compared to the control samples. Juices supplementation with CESP significantly decreased their acidity and total organic acids content as well as increased their pH value. Chokeberry and cranberry juices supplementation with 1% CESP caused no significant changes in the amount of precipitate and their color, but it significantly improved their taste. For this reason, CESP addition in the amount of up to 1% can be suggested as the optimal supplementation of berry fruit juices. The study also demonstrated that CESP addition in the amount of up to 1% caused no significant differences in the content of polyphenolic compounds and in the antioxidative activity of juices, which can be deemed important from the viewpoint of their putative health benefits. In addition, the heat treatment of juices contributed to only a 4% loss of polyphenolic compounds from the CESP-supplemented juices compared to the 6% loss from the non-supplemented juices.
Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater
Authors: Navarro-López Elvira , Ruíz-Nieto Angela , Ferreira Alice , Acién F. Gabriel , Gouveia Luisa
Published: February 2020

Microalgae are microorganisms with the capacity to contribute to the sustainable and healthy food production, in addition to wastewater treatment. The subject of this work was to determine the potential of Scenedesmus obliquus microalga grown in brewery wastewater to act as a plant biostimulant. The germination index of watercress seeds, as well as the auxin-like activity in mung bean and cucumber, and in the cytokinin-like activity in cucumber bioassays were used to evaluate the biostimulant potential. Several biomass processes were studied, such as centrifugation, ultrasonication and enzymatic hydrolysis, as well as the final concentration of microalgal extracts to determine their influence in the biostimulant activity of the Scenedesmus biomass. The results showed an increase of 40% on the germination index when using the biomass at 0.1 g/L, without any pre-treatment. For auxin-like activity, the best results (up to 60% with respect to control) were obtained at 0.5 g/L of biomass extract, after a combination of cell disruption, enzymatic hydrolysis and centrifugation. For cytokinin-like activity, the best results (up to 187.5% with respect to control) were achieved without cell disruption, after enzymatic hydrolysis and centrifugation at a biomass extract concentration of 2 g/L.
Biological Evaluation and In Silico Study of Benzoic Acid Derivatives from Bjerkandera adusta Targeting Proteostasis Network Modules
Authors: Georgousaki Katerina , Tsafantakis Nikolaos , Gumeni Sentiljana , Lambrinidis George , González-Menéndez Victor , Tormo Jose R. , Genilloud Olga , Trougakos Ioannis P. , Fokialakis Nikolas
Published: February 2020

A main cellular functional module that becomes dysfunctional during aging is the proteostasis network. In the present study, we show that benzoic acid derivatives isolated from Bjerkandera adusta promote the activity of the two main protein degradation systems, namely the ubiquitin-proteasome (UPP) and especially the autophagy-lysosome pathway (ALP) in human foreskin fibroblasts. Our findings were further supported by in silico studies, where all compounds were found to be putative binders of both cathepsins B and L. Among them, compound 3 (3-chloro-4-methoxybenzoic acid) showed the most potent interaction with both enzymes, which justifies the strong activation of cathepsins B and L (467.3 ± 3.9%) on cell-based assays. Considering that the activity of both the UPP and ALP pathways decreases with aging, our results suggest that the hydroxybenzoic acid scaffold could be considered as a promising candidate for the development of novel modulators of the proteostasis network, and likely of anti-aging agents.
Recent Progress in Nitro-Promoted Direct Functionalization of Pyridones and Quinolones
Authors: Hao Feiyue , Nishiwaki Nagatoshi
Published: February 2020

Nitro group is one of the most important functional groups in organic syntheses because its strongly electron-withdrawing ability activates the scaffold, facilitating the reaction with nucleophilic reagents or the Diels–Alder reaction. In this review, recent progress in the nitro-promoted direct functionalization of pyridones and quinolones is highlighted to complement previous reviews.
Role of β-Caryophyllene in the Antinociceptive and Anti-Inflammatory Effects of Tagetes lucida Cav. Essential Oil
Authors: Hernandez-Leon Alberto , González-Trujano María Eva , Narváez-González Fernando , Pérez-Ortega Gimena , Rivero-Cruz Fausto , Aguilar María Isabel
Published: February 2020

Tagetes lucida Cav. (Asteraceae) is an ancient medicinal plant commonly used to alleviate pain. Nevertheless, scientific studies validating this property are lacking in the literature. Animal models of pain were used to evaluate the antinociceptive and anti-inflammatory activities of T. lucida essential oil (TLEO) and a bioactive metabolite. The chemical constitution and possible toxicity of the extract and the mechanism of action of β-caryophyllene were also explored. Temporal course curves and dose–response graphics were generated using TLEO (0.1–10 mg/kg or 3.16–31.62 mg/kg) and β-caryophyllene (3.16–10 mg/kg). Metamizole (80 mg/kg) and indomethacin (20 mg/kg) were used as reference drugs in the formalin assay and writhing test in rats and mice, respectively. The β-caryophyllene mechanism of action was explored in the presence of naloxone (1 mg/kg), flumazenil (10 mg/kg), WAY100635 (0.16 mg/kg), or nitro-l-arginine methyl ester (L-NAME) (20 mg/kg) in the formalin test in rats. GC/MS analysis demonstrated the presence of geranyl acetate (49.89%), geraniol (7.92%), and β-caryophyllene (6.27%). Significant and dose-dependent antinociceptive response was produced by TLEO and β-caryophyllene without the presence of gastric damage. In conclusion, β-caryophyllene was confirmed as a bioactive compound in the T. lucida analgesic properties by involving the participation of receptors like opioids, benzodiazepines, and Serotonin 1A receptor (5-HT1A), as well as nitric oxide.
Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors
Authors: Vu Cao-An , Chen Wen-Yih
Published: February 2020

Aptamers, in sensing technology, are famous for their role as receptors in versatile applications due to their high specificity and selectivity to a wide range of targets including proteins, small molecules, oligonucleotides, metal ions, viruses, and cells. The outburst of field-effect transistors provides a label-free detection and ultra-sensitive technique with significantly improved results in terms of detection of substances. However, their combination in this field is challenged by several factors. Recent advances in the discovery of aptamers and studies of Field-Effect Transistor (FET) aptasensors overcome these limitations and potentially expand the dominance of aptamers in the biosensor market.
Synthesis of Functionalized Cannabilactones
Authors: Liu Yingpeng , Ho Thanh C. , Baradwan Mohammed , Pascual Lopez-Alberca Maria , Iliopoulos-Tsoutsouvas Christos , Nikas Spyros P. , Makriyannis Alexandros
Published: February 2020

A new approach to synthesize cannabilactones using Suzuki cross-coupling reaction followed by one-step demethylation-cyclization is presented. The two key cannabilactone prototypes AM1710 and AM1714 were obtained selectively in high overall yields and in a lesser number of synthetic steps when compared to our earlier synthesis. The new approach expedited the synthesis of cannabilactone analogs with structural modifications at the four potential pharmacophoric regions.
New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples
Authors: Casado Natalia , Gañán Judith , Morante-Zarcero Sonia , Sierra Isabel
Published: February 2020

Natural toxins are chemical substances that are not toxic to the organisms that produce them, but which can be a potential risk to human health when ingested through food. Thus, it is of high interest to develop advanced analytical methodologies to control the occurrence of these compounds in food products. However, the analysis of food samples is a challenging task because of the high complexity of these matrices, which hinders the extraction and detection of the analytes. Therefore, sample preparation is a crucial step in food analysis to achieve adequate isolation and/or preconcentration of analytes and provide suitable clean-up of matrix interferences prior to instrumental analysis. Current trends in sample preparation involve moving towards “greener” approaches by scaling down analytical operations, miniaturizing the instruments and integrating new advanced materials as sorbents. The combination of these new materials with sorbent-based microextraction technologies enables the development of high-throughput sample preparation methods, which improve conventional extraction and clean-up procedures. This review gives an overview of the most relevant analytical strategies employed for sorbent-based microextraction of natural toxins of exogenous origin from food, as well as the improvements achieved in food sample preparation by the integration of new advanced materials as sorbents in these microextraction techniques, giving some relevant examples from the last ten years. Challenges and expected future trends are also discussed.
Impact of Engineered Carbon Nanodiamonds on the Collapse Mechanism of Model Lung Surfactant Monolayers at the Air-Water Interface
Authors: Chakraborty Aishik , Hertel Amanda , Ditmars Hayley , Dhar Prajnaparamita
Published: February 2020

Understanding interactions between inhaled nanoparticles and lung surfactants (LS) present at the air-water interface in the lung, is critical to assessing the toxicity of these nanoparticles. Specifically, in this work, we assess the impact of engineered carbon nanoparticles (ECN) on the ability of healthy LS to undergo reversible collapse, which is essential for proper functioning of LS. Using a Langmuir trough, multiple compression-expansion cycles are performed to assess changes in the surface pressure vs. area isotherms with time and continuous cyclic compression-expansion. Further, theoretical analysis of the isotherms is used to calculate the ability of these lipid systems to retain material during monolayer collapse, due to interactions with ECNs. These results are complemented with fluorescence images of alterations in collapse mechanisms in these monolayer films. Four different model phospholipid systems, that mimic the major compositions of LS, are used in this study. Together, our results show that the ECN does not impact the mechanism of collapse. However, the ability to retain material at the interface during monolayer collapse, as well as re-incorporation of material after a compression-expansion cycle is altered to varying extent by ECNs and depends on the composition of the lipid mixtures.
Cell-Based Nanoparticles Delivery Systems for Targeted Cancer Therapy: Lessons from Anti-Angiogenesis Treatments
Authors: de la Torre Paz , Pérez-Lorenzo María Jesús , Alcázar-Garrido Álvaro , Flores Ana I.
Published: February 2020

The main strategy of cancer treatment has focused on attacking the tumor cells. Some cancers initially responsive to chemotherapy become treatment-resistant. Another strategy is to block the formation of tumor vessels. However, tumors also become resistant to anti-angiogenic treatments, mostly due to other cells and factors present in the tumor microenvironment, and hypoxia in the central part of the tumor. The need for new cancer therapies is significant. The use of nanoparticle-based therapy will improve therapeutic efficacy and targeting, while reducing toxicity. However, due to inefficient accumulation in tumor sites, clearance by reticuloendothelial organs and toxicity, internalization or conjugation of drug-loaded nanoparticles (NPs) into mesenchymal stem cells (MSCs) can increase efficacy by actively delivering them into the tumor microenvironment. Nanoengineering MSCs with drug-loaded NPs can increase the drug payload delivered to tumor sites due to the migratory and homing abilities of MSCs. However, MSCs have some disadvantages, and exosomes and membranes from different cell types can be used to transport drug-loaded NPs actively to tumors. This review gives an overview of different cancer approaches, with a focus on hypoxia and the emergence of NPs as drug-delivery systems and MSCs as cellular vehicles for targeted delivery due to their tumor-homing potential.
Development of Coumarin-Based Hydroxamates as Histone Deacetylase Inhibitors with Antitumor Activities
Authors: Zhao Na , Yang Feifei , Han Lina , Qu Yuhua , Ge Di , Zhang Hua
Published: February 2020

Histone deacetylases (HDACs) have been proved to be promising targets for the treatment of cancer, and five histone deacetylase inhibitors (HDACis) have been approved on the market for the treatment of different lymphomas. In our previous work, we designed a series of novel coumarin-containing hydroxamate HDACis, among which compounds 6 and 7 displayed promising activities against tumor growth. Based on a molecular docking study, we further developed 26 additional analogues with the aim to improve activity of designed compounds. Several of these new derivatives not only showed excellent HDAC1 inhibitory effects, but also displayed significant growth inhibitory activities against four human cancer cell lines. Representative compounds, 13a and 13c, showed potent anti-proliferative activities against solid tumor cell lines with IC50 values of 0.36–2.91 µM and low cytotoxicity against Beas-2B and L-02 normal cells. Immunoblot analysis revealed that 13a and 13c dose-dependently increased the acetylation of histone H3 and H4. Importantly, the two compounds displayed much better anti-metastatic effects than SAHA against the MDA-MB-231 cell line. Moreover, 13a and 13c arrested MDA-MB-231 cells at G2/M phase and induced MDA-MB-231 cell apoptosis. Finally, the molecular docking study rationalized the high potency of compound 13c.
Development of RNA/DNA Hydrogel Targeting Toll-Like Receptor 7/8 for Sustained RNA Release and Potent Immune Activation
Authors: Komura Fusae , Okuzumi Kana , Takahashi Yuki , Takakura Yoshinobu , Nishikawa Makiya
Published: February 2020

Guanosine- and uridine-rich single-stranded RNA (GU-rich RNA) is an agonist of Toll-like receptor (TLR) 7 and TLR8 and induces strong immune responses. A nanostructured GU-rich RNA/DNA assembly prepared using DNA nanotechnology can be used as an adjuvant capable of improving the biological stability of RNA and promoting efficient RNA delivery to target immune cells. To achieve a sustained supply of GU-rich RNA to immune cells, we developed a GU-rich RNA/DNA hydrogel (RDgel) using nanostructured GU-rich RNA/DNA assembly, from which GU-rich RNA can be released in a sustained manner. A hexapod-like GU-rich RNA/DNA nanostructure, or hexapodRD6, was designed using a 20-mer phosphorothioate-stabilized GU-rich RNA and six phosphodiester DNAs. Two sets of hexapodRD6 were mixed to obtain RDgel. Under serum-containing conditions, GU-rich RNA was gradually released from the RDgel. Fluorescently labeled GU-rich RNA was efficiently taken up by DC2.4 murine dendritic cells and induced a high level of tumor necrosis factor-α release from these cells when it was incorporated into RDgel. These results indicate that the RDgel constructed using DNA nanotechnology can be a useful adjuvant in cancer therapy with sustained RNA release and high immunostimulatory activity.
Genotoxicity and Cytotoxicity of Gold Nanoparticles In Vitro: Role of Surface Functionalization and Particle Size
Authors: Vales Gerard , Suhonen Satu , Siivola Kirsi M. , Savolainen Kai M. , Catalán Julia , Norppa Hannu
Published: February 2020

Several studies suggested that gold nanoparticles (NPs) could be genotoxic in vitro and in vivo. However, gold NPs have currently produced present a wide range of sizes and functionalization, which could affect their interactions with the environment or with biological structures and, thus, modify their toxic effects. In this study, we investigated the role of surface charge in determining the genotoxic potential of gold NPs, as measured by the induction of DNA damage (comet assay) and chromosomal damage (micronucleus assay) in human bronchial epithelial BEAS-2B cells. The cellular uptake of gold NPs was assessed by hyperspectral imaging. Two core sizes (~5 nm and ~20 nm) and three functionalizations representing negative (carboxylate), positive (ammonium), and neutral (poly(ethylene glycol); (PEG)ylated) surface charges were examined. Cationic ammonium gold NPs were clearly more cytotoxic than their anionic and neutral counterparts, but genotoxicity was not simply dependent on functionalization or size, since DNA damage was induced by 20-nm ammonium and PEGylated gold NPs, while micronucleus induction was increased by 5-nm ammonium and 20-nm PEGylated gold NPs. The 5-nm carboxylated gold NPs were not genotoxic, and evidence on the genotoxicity of the 20-nm carboxylated gold NPs was restricted to a positive result at the lowest dose in the micronucleus assay. When interpreting the results, it has to be taken into account that cytotoxicity limited the doses available for the ammonium-functionalized gold NPs and that gold NPs have earlier been described to interfere with the comet assay procedure, possibly resulting in a false positive result. In conclusion, our findings show that the cellular uptake and cytotoxicity of gold NPs are clearly enhanced by positive surface charge, but neither functionalization nor size can single-handedly account for the genotoxic effects of the gold NPs.
6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection
Authors: Deng Hao-Hua , Shi Xiao-Qiong , Balasubramanian Paramasivam , Huang Kai-Yuan , Xu Ying-Ying , Huang Zhong-Nan , Peng Hua-Ping , Chen Wei
Published: February 2020

This study puts forward an efficient method for protein detection in virtue of the tremendous fluorescence enhancement property of 6-aza-2-thio-thymine protected gold nanoclusters (ATT-AuNCs). In-depth studies of the protein-induced photoluminescence enhancement mechanism illustrate the mechanism of the interaction between ATT-AuNCs and protein. This new-established probe enables feasible and sensitive quantification of the concentrations of total protein in real samples, such as human serum, human plasma, milk, and cell extracts. The results of this proposed method are in good agreement with those determined by the classical bicinchoninic acid method (BCA method).
Reviewing the Composition of Vaginal Microbiota: Inclusion of Nutrition and Probiotic Factors in the Maintenance of Eubiosis
Authors: Barrientos-Durán Antonio , Fuentes-López Ana , de Salazar Adolfo , Plaza-Díaz Julio , García Federico
Published: February 2020

The vaginal microbiota has importance in preserving vaginal health and defending the host against disease. The advent of new molecular techniques and computer science has allowed researchers to discover microbial composition in depth and associate the structure of vaginal microbial communities. There is a consensus that vaginal flora is grouped into a restricted number of communities, although the structure of the community is constantly changing. Certain Community-State Types (CSTs) are more associated with poor reproductive outcomes and sexually transmitted diseases (STDs) meanwhile, CSTs dominated by Lactobacillus species—particularly Lactobacillus crispatus—are more related to vaginal health. In this work, we have reviewed how modifiable and non-modifiable factors may affect normal vaginal microbiota homeostasis—including sexual behavior, race or ethnicity, and hygiene. Special interest has been given to how the use of probiotics, diet intake, and use of hormone replacement therapies (HRTs) can potentially impact vaginal microbiota composition.