bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review of Atmosphere–Ocean Forcings Outside the Tropical Pacific on the El Niño–Southern Oscillation Occurrence
Authors: Chen Shangfeng , Yu Bin , Chen Wen , Wu Renguang
Published: November 2018

The El Niño–Southern Oscillation (ENSO) is the strongest interannual air–sea coupled variability mode in the tropics, and substantially impacts the global weather and climate. Hence, it is important to improve our understanding of the ENSO variability. Besides the well-known air–sea interaction process over the tropical Pacific, recent studies indicated that atmospheric and oceanic forcings outside the tropical Pacific also play important roles in impacting and modulating the ENSO occurrence. This paper reviews the impacts of the atmosphere–ocean variability outside the tropical Pacific on the ENSO variability, as well as their associated physical processes. The review begins with the contribution of the atmosphere–ocean forcings over the extratropical North Pacific, Atlantic, and Indian Ocean on the ENSO occurrence. Then, an overview of the extratropical atmospheric forcings over the Northern Hemisphere (including the Arctic Oscillation and the Asian monsoon systems) and the Southern Hemisphere (including the Antarctic Oscillation and the Pacific–South American teleconnection), on the ENSO occurrence, is presented. It is shown that the westerly (easterly) wind anomaly over the tropical western Pacific is essential for the occurrence of an El Niño (a La Niña) event. The wind anomalies over the tropical western Pacific also play a key role in relaying the impacts of the atmosphere–ocean forcings outside the tropical Pacific on the ENSO variability. Finally, some relevant questions, that remain to be explored, are discussed.
On the Roles of Advection and Solar Heating in Seasonal Variation of the Migrating Diurnal Tide in the Stratosphere, Mesosphere, and Lower Thermosphere
Authors: Gu Hongping , Du Jian
Published: November 2018

The migrating diurnal tide (DW1) presents a unique latitudinal structure in the stratosphere, mesosphere, and lower thermosphere. In this paper, the physical mechanisms that govern its seasonal variation are examined in these three regions using the 31.5-year (1979–2010) output from the extended Canadian Middle Atmosphere Model (eCMAM30). DW1 annual variation in the stratosphere is mainly controlled by the short-wave heating in the high latitudes, but by both the short-wave and adiabatic heating in the low latitudes. In the mesosphere, linear and nonlinear advection play important roles in the semiannual variation of the tide whereas short-wave heating does not. In the lower thermosphere, the annual variation of DW1 is mainly governed by the short-wave heating and linear advection. This study illustrates the complexity of the main physical mechanisms modulating the seasonal variations of DW1 in different regions of the atmosphere.
Application of Neutrosophic Soft Sets to K-Algebras
Authors: Akram Muhammad , Gulzar Hina , Smarandache Florentin , Broumi Said
Published: November 2018

Neutrosophic sets and soft sets are two different mathematical tools for representing vagueness and uncertainty. We apply these models in combination to study vagueness and uncertainty in K-algebras. We introduce the notion of single-valued neutrosophic soft (SNS) K-algebras and investigate some of their properties. We establish the notion of ( ∈ , ∈ ∨ q ) -single-valued neutrosophic soft K-algebras and describe some of their related properties. We also illustrate the concepts with numerical examples.
Synthesis of a Novel α-Glucosyl Ginsenoside F1 by Cyclodextrin Glucanotransferase and Its In Vitro Cosmetic Applications
Authors: Moon Seong Soo , Lee Hye Jin , Mathiyalagan Ramya , Kim Yu Jin , Yang Dong Uk , Lee Dae Young , Min Jin Woo , Jimenez Zuly , Yang Deok Chun
Published: November 2018

Ginsenosides from Panax ginseng (Korean ginseng) are unique triterpenoidal saponins that are considered to be responsible for most of the pharmacological activities of P. ginseng. However, the various linkage positions cause different pharmacological activities. In this context, we aimed to synthesize new derivatives of ginsenosides with unusual linkages that show enhanced pharmacological activities. Novel α-glycosylated derivatives of ginsenoside F1 were synthesized from transglycosylation reactions of dextrin (sugar donor) and ginsenoside F1 (acceptor) by the successive actions of Toruzyme®3.0L, a cyclodextrin glucanotransferase. One of the resultant products was isolated and identified as (20S)-3β,6α,12β-trihydroxydammar-24ene-(20-O-β-D-glucopyranosyl-(1→2)-α-D-glucopyranoside) by various spectroscopic characterization techniques of fast atom bombardment-mass spectrometry (FAB-MS), infrared spectroscopy (IR), proton-nuclear magnetic resonance (1H-NMR), 13C-NMR, gradient heteronuclear single quantum coherence (gHSQC), and gradient heteronuclear multiple bond coherence (gHMBC). As expected, the novel α-glycosylated ginsenoside F1 (G1-F1) exhibited increased solubility, lower cytotoxicity toward human dermal fibroblast cells (HDF), and higher tyrosinase activity and ultraviolet A (UVA)-induced inhibitory activity against matrix metalloproteinase-1 (MMP-1) than ginsenoside F1. Since F1 has been reported as an antiaging and antioxidant agent, the enhanced efficacies of the novel α-glycosylated ginsenoside F1 suggest that it might be useful in cosmetic applications after screening.
Exploring Solanum tuberosum Epoxide Hydrolase Internal Architecture by Water Molecules Tracking
Authors: Mitusińska Karolina , Magdziarz Tomasz , Bzówka Maria , Stańczak Agnieszka , Góra Artur
Published: November 2018

Several different approaches are used to describe the role of protein compartments and residues in catalysis and to identify key residues suitable for the modification of the activity or selectivity of the desired enzyme. In our research, we applied a combination of molecular dynamics simulations and a water tracking approach to describe the water accessible volume of Solanum tuberosum epoxide hydrolase. Using water as a molecular probe, we were able to identify small cavities linked with the active site: (i) one made up of conserved amino acids and indispensable for the proper positioning of catalytic water and (ii) two others in which modification can potentially contribute to enzyme selectivity and activity. Additionally, we identified regions suitable for de novo tunnel design that could also modify the catalytic properties of the enzyme. The identified hot-spots extend the list of the previously targeted residues used for modification of the regioselectivity of the enzyme. Finally, we have provided an example of a simple and elegant process for the detailed description of the network of cavities and tunnels, which can be used in the planning of enzyme modifications and can be easily adapted to the study of any other protein.
Oxidation of 5-methylaminomethyl uridine (mnm5U) by Oxone Leads to Aldonitrone Derivatives
Authors: Zhou Qishun , Vu Ngoc Bao Tram , Leszczynska Grazyna , Stigliani Jean-Luc , Pratviel Geneviève
Published: November 2018

Oxidative RNA damage is linked to cell dysfunction and diseases. The present work focuses on the in vitro oxidation of 5-methylaminomethyl uridine (mnm5U), which belongs to the numerous post-transcriptional modifications that are found in tRNA. The reaction of oxone with mnm5U in water at pH 7.5 leads to two aldonitrone derivatives. They form by two oxidation steps and one dehydration step. Therefore, the potential oxidation products of mnm5U in vivo may not be only aldonitrones, but also hydroxylamine and imine derivatives (which may be chemically more reactive). Irradiation of aldonitrone leads to unstable oxaziridine derivatives that are susceptible to isomerization to amide or to hydrolysis to aldehyde derivative.
Cardiac Targeting Peptide, a Novel Cardiac Vector: Studies in Bio-Distribution, Imaging Application, and Mechanism of Transduction
Authors: Zahid Maliha , Feldman Kyle S. , Garcia-Borrero Gabriel , Feinstein Timothy N. , Pogodzinski Nicholas , Xu Xinxiu , Yurko Raymond , Czachowski Michael , Wu Yijen L. , Mason Neale S. , Lo Cecilia W.
Published: November 2018

Our previous work identified a 12-amino acid peptide that targets the heart, termed cardiac targeting peptide (CTP). We now quantitatively assess the bio-distribution of CTP, show a clinical application with the imaging of the murine heart, and study its mechanisms of transduction. Bio-distribution studies of cyanine5.5-N-Hydroxysuccinimide (Cy5.5) labeled CTP were undertaken in wild-type mice. Cardiac targeting peptide was labeled with Technetium 99m (99mTc) using the chelator hydrazino-nicotinamide (HYNIC), and imaging performed using micro-single photon emission computerized tomography/computerized tomography (SPECT/CT). Human-induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMCs) were incubated with dual-labeled CTP, and imaged using confocal microscopy. TriCEPs technology was utilized to study the mechanism of transduction. Bio-distribution studies showed peak uptake of CTP at 15 min. 99mTc-HYNIC-CTP showed heart-specific uptake. Robust transduction of beating human iPSC-derived CMCs was seen. TriCEPs experiments revealed five candidate binding partners for CTP, with Kcnh5 being felt to be the most likely candidate as it showed a trend towards being competed out by siRNA knockdown. Transduction efficiency was enhanced by increasing extracellular potassium concentration, and with Quinidine, a Kcnh5 inhibitor, that blocks the channel in an open position. We demonstrate that CTP transduces the normal heart as early as 15 min. 99mTc-HYNIC-CTP targets the normal murine heart with substantially improved targeting compared with 99mTc Sestamibi. Cardiac targeting peptide’s transduction ability is not species limited and has human applicability. Cardiac targeting peptide appears to utilize Kcnh5 to gain cell entry, a phenomenon that is affected by pre-treatment with Quinidine and changes in potassium levels.
Raman Spectroscopy and Microscopy Applications in Cardiovascular Diseases: From Molecules to Organs
Authors: Chaichi Ardalan , Prasad Alisha , Gartia Manas Ranjan
Published: November 2018

Noninvasive and label-free vibrational spectroscopy and microscopy methods have shown great potential for clinical diagnosis applications. Raman spectroscopy is based on inelastic light scattering due to rotational and vibrational modes of molecular bonds. It has been shown that Raman spectra provide chemical signatures of changes in biological tissues in different diseases, and this technique can be employed in label-free monitoring and clinical diagnosis of several diseases, including cardiovascular studies. However, there are very few literature reviews available to summarize the state of art and future applications of Raman spectroscopy in cardiovascular diseases, particularly cardiac hypertrophy. In addition to conventional clinical approaches such as electrocardiography (ECG), echocardiogram (cardiac ultrasound), positron emission tomography (PET), cardiac computed tomography (CT), and single photon emission computed tomography (SPECT), applications of vibrational spectroscopy and microscopy will provide invaluable information useful for the prevention, diagnosis, and treatment of cardiovascular diseases. Various in vivo and ex vivo investigations can potentially be performed using Raman imaging to study and distinguish pathological and physiological cardiac hypertrophies and understand the mechanisms of other cardiac diseases. Here, we have reviewed the recent literature on Raman spectroscopy to study cardiovascular diseases covering investigations on the molecular, cellular, tissue, and organ level.
Post-Occupancy Evaluation and IEQ Measurements from 64 Office Buildings: Critical Factors and Thresholds for User Satisfaction on Thermal Quality
Authors: Park Jihyun , Loftness Vivian , Aziz Azizan
Published: November 2018

The indoor environmental quality (IEQ) of buildings can have a strong influence on occupants’ comfort, productivity, and health. Post-occupancy evaluation (POE) is necessary in assessing the IEQ of the built environment, and it typically relies on the subjective surveys of thermal quality, air quality, visual quality, and acoustic quality. In this research, we expanded POE to include both objective IEQ measurements and the technical attributes of building systems (TABS) that may affect indoor environment and user satisfaction. The suite of three tools, including user satisfaction survey, workstation IEQ measurements, and TABS in the National Environmental Assessment Toolkit (NEAT) has been deployed in 1601 workstations in 64 office buildings, generating a rich database for statistical evaluation of possible correlations between the physical attributes of workstations, environmental conditions, and user satisfaction. Multivariate regression and multiple correlation coefficient statistical analysis revealed the relationship between measured and perceived IEQ indices, interdependencies between IEQ indices, and other satisfaction variables of significance. The results showed that overall, 55% of occupants responded as “satisfied” or “neutral”, and 45% reported being “dissatisfied” in their thermal quality. Given the dataset, air temperature in work area, size of thermal zone, window quality, level of temperature control, and radiant temperature asymmetry with façade are the critical factors for thermal quality satisfaction in the field. As a result, the outcome of this research contributes to identifying correlations between occupant satisfaction, measured data, and technical attributes of building systems. The presented integrated IEQ assessment method can further afford robust predictions of building performance against metrics and guidelines for IEQ standards to capture revised IEQ thresholds that impact building occupants’ satisfaction.
Creating a Market for Disability Specific Housing on Indigenous Land: A Case Study from Yarrabah, Australia
Authors: Sarkodee Kate , Martel Andrew
Published: November 2018

Australia’s National Disability Insurance Scheme Specialist Disability Accommodation (NDIS SDA) program anticipates new, disability specific, housing stock being built by private investors incentivized by cash payments and rental income. To date, very few new SDA dwellings have been constructed and the majority of the research and analysis of the program’s potential has been in the context of apartment construction in major capital city markets in Australia. This paper uses a hypothetical case study of building SDA accommodation in a discrete regional Indigenous community, Yarrabah, in Queensland. It investigates underlying assumptions within the scheme, particularly around the relationship of land to investment outcomes, as well as cultural considerations. An important aspect is to test how effectively the design guidelines associated with the scheme translate into an appropriate built form that is culturally and environmentally appropriate in locations outside major urban centres. The results suggest that housing actors from the not-for-profit sector may benefit from the SDA at the expense of profit-driven, market-based housing developers, and that the SDA design categories offer limited flexibility for participants with changing care needs, potentially restricting resident continuity in occupancy and ongoing return on investment. The work offers an early assessment on the workability of the SDA in the context of housing investment in a new market for the private housing industry.
The Dilemma of Cure and Damage in Oligodendroglioma: Ways to Tip the Balance Away from the Damage
Authors: Torensma Ruurd
Published: November 2018

Current treatments for oligodendrogliomas are powerful but have a negative impact on the rest of the body. The bone marrow is damaged by the chemotherapeutics, but other parts of the body are also affected. In this paper, the current treatment method and its collateral damage is described. Therefore, therapies are needed that are more effective against the tumor while having less negative effects on the patient’s quality of life. Some potential therapies include optimal removal of the tumor by fluorescent-guided surgery (FGS), intraoperative desorption electrospray ionization-mass spectrometry (DESI-MS), better monitoring of the effects of therapy by pseudo-coloring shades of gray of MRI pictures, and using recent data from RNA sequencing of single cells and immunotherapy. These are all open new ways of treating this tumor. The RNA sequencing of single tumor cells unravels specific tumor antigens present in the differentiation status of the cancer cell. Stem cell antigens were expressed in dividing cells, while hypoxia inducible factor-α (HIF-1α) is expressed in all tumor cells. Cancer stem cell antigens can be loaded on dendritic cells to induce cytotoxic T-cells directed to cancer stem cells. These recent discoveries suggest a better quality of life with the same overall survival.
Better Progression-Free Survival in Elderly Patients with Stage IV Lung Adenocarcinoma Harboring Uncommon Epidermal Growth Factor Receptor Mutations Treated with the First-line Tyrosine Kinase Inhibitors
Authors: Tsai Ming-Ju , Hung Jen-Yu , Lee Mei-Hsuan , Kuo Chia-Yu , Tsai Yu-Chen , Tsai Ying-Ming , Liu Ta-Chih , Yang Chih-Jen , Huang Ming-Shyan , Chong Inn-Wen
Published: November 2018

Patients with lung adenocarcinoma harboring common epidermal growth factor receptor (EGFR) mutations usually have a good response rate (RR) and longer progression-free survival (PFS) to EGFR tyrosine kinase inhibitors (TKIs). However, the treatment efficacy to uncommon EGFR mutations remains controversial. We, therefore, performed a retrospective study, screening 2958 patients. A total of 67 patients with lung adenocarcinoma harboring uncommon EGFR mutations were enrolled and 57 patients with stage IV diseases receiving a first-line EGFR TKI were included for further analyses. The patients were classified into 27 (47%) “a single sensitizing uncommon mutation”, 7 (12%) “multiple sensitizing mutations”, 5 (9%) “a sensitizing mutation and a resistant uncommon mutation”, and 18 (32%) “other resistant uncommon mutations”. No significant difference was noted in PFS or overall survival (OS) between groups. Patients receiving different first-line EGFR TKIs had similar PFS and OS. The elder patients had a significantly poorer performance status than the younger patients but a significantly longer PFS than the younger patients (median PFS: 10.5 vs. 5.5 months, p = 0.0320). In conclusion, this is the first study to identify that elderly patients with stage IV lung adenocarcinoma harboring uncommon EGFR mutation might have a longer PFS. Large-scale prospective studies are mandatory to prove our findings.
Humanization of the Prostate Microenvironment Reduces Homing of PC3 Prostate Cancer Cells to Human Tissue-Engineered Bone
Authors: McGovern Jacqui , Shafiee Abbas , Wagner Ferdinand , Lahr Christoph , Landgraf Marietta , Meinert Christoph , Williams Elizabeth , Russell Pamela , Clements Judith , Loessner Daniela , Holzapfel Boris , Risbridger Gail , Hutmacher Dietmar
Published: November 2018

The primary tumor microenvironment is inherently important in prostate cancer (PCa) initiation, growth and metastasis. However, most current PCa animal models are based on the injection of cancer cells into the blood circulation and bypass the first steps of the metastatic cascade, hence failing to investigate the influence of the primary tumor microenvironment on PCa metastasis. Here, we investigated the spontaneous metastasis of PC3 human PCa cells from humanized prostate tissue, containing cancer-associated fibroblasts (CAFs) and prostate lymphatic and blood vessel endothelial cells (BVECs), to humanized tissue-engineered bone constructs (hTEBCs) in NOD-SCID IL2Rγnull (NSG) mice. The hTEBC formed a physiologically mature organ bone which allowed homing of metastatic PCa cells. Humanization of prostate tissue had no significant effect on the tumor burden at the primary site over the 4 weeks following intraprostatic injection, yet reduced the incidence and burden of metastases in the hTEBC. Spontaneous PCa metastases were detected in the lungs and spleen with no significant differences between the humanized and non-humanized prostate groups. A significantly greater metastatic tumor burden was observed in the liver when metastasis occurred from the humanized prostate. Together, our data suggests that the presence of human-derived CAFs and BVECs in the primary PCa microenvironment influences selectively the metastatic and homing behavior of PC3 cells in this model. Our orthotopic and humanized PCa model developed via convergence of cancer research and tissue engineering concepts provides a platform to dissect mechanisms of species-specific PCa bone metastasis and to develop precision medicine strategies.
The Role of the Anti-Aging Protein Klotho in IGF-1 Signaling and Reticular Calcium Leak: Impact on the Chemosensitivity of Dedifferentiated Liposarcomas
Authors: Delcroix Vanessa , Mauduit Olivier , Tessier Nolwenn , Montillaud Anaïs , Lesluyes Tom , Ducret Thomas , Chibon Frédéric , Van Coppenolle Fabien , Ducreux Sylvie , Vacher Pierre
Published: November 2018

By inhibiting Insulin-Like Growth Factor-1-Receptor (IGF-1R) signaling, Klotho (KL) acts like an aging- and tumor-suppressor. We investigated whether KL impacts the aggressiveness of liposarcomas, in which IGF-1R signaling is frequently upregulated. Indeed, we observed that a higher KL expression in liposarcomas is associated with a better outcome for patients. Moreover, KL is downregulated in dedifferentiated liposarcomas (DDLPS) compared to well-differentiated tumors and adipose tissue. Because DDLPS are high-grade tumors associated with poor prognosis, we examined the potential of KL as a tool for overcoming therapy resistance. First, we confirmed the attenuation of IGF-1-induced calcium (Ca2+)-response and Extracellular signal-Regulated Kinase 1/2 (ERK1/2) phosphorylation in KL-overexpressing human DDLPS cells. KL overexpression also reduced cell proliferation, clonogenicity, and increased apoptosis induced by gemcitabine, thapsigargin, and ABT-737, all of which are counteracted by IGF-1R-dependent signaling and activate Ca2+-dependent endoplasmic reticulum (ER) stress. Then, we monitored cell death and cytosolic Ca2+-responses and demonstrated that KL increases the reticular Ca2+-leakage by maintaining TRPC6 at the ER and opening the translocon. Only the latter is necessary for sensitizing DDLPS cells to reticular stressors. This was associated with ERK1/2 inhibition and could be mimicked with IGF-1R or MEK inhibitors. These observations provide a new therapeutic strategy in the management of DDLPS.
Spectrum and Prevalence of Pathogenic Variants in Ovarian Cancer Susceptibility Genes in a Group of 333 Patients
Authors: Koczkowska Magdalena , Krawczynska Natalia , Stukan Maciej , Kuzniacka Alina , Brozek Izabela , Sniadecki Marcin , Debniak Jaroslaw , Wydra Dariusz , Biernat Wojciech , Kozlowski Piotr , Limon Janusz , Wasag Bartosz , Ratajska Magdalena
Published: November 2018

Constitutional loss-of-function pathogenic variants in the tumor suppressor genes BRCA1 and BRCA2 are widely associated with an elevated risk of ovarian cancer (OC). As only ~15% of OC individuals carry the BRCA1/2 pathogenic variants, the identification of other potential OC-susceptibility genes is of great clinical importance. Here, we established the prevalence and spectrum of the germline pathogenic variants in the BRCA1/2 and 23 other cancer-related genes in a large Polish population of 333 unselected OC cases. Approximately 21% of cases (71/333) carried the BRCA1/2 pathogenic or likely pathogenic variants, with c.5266dup (p.Gln1756Profs*74) and c.3700_3704del (p.Val1234Glnfs*8) being the most prevalent. Additionally, ~6% of women (20/333) were carriers of the pathogenic or likely pathogenic variants in other cancer-related genes, with NBN and CHEK2 reported as the most frequently mutated, accounting for 1.8% (6/333) and 1.2% (4/333) of cases, respectively. We also found ten pathogenic or likely pathogenic variants in other genes: 1/333 in APC, 1/333 in ATM, 2/333 in BLM, 1/333 in BRIP1, 1/333 in MRE11A, 2/333 in PALB2, 1/333 in RAD50, and 1/333 in RAD51C, accounting for 50% of all detected variants in moderate- and low-penetrant genes. Our findings confirmed the presence of the additional OC-associated genes in the Polish population that may improve the personalized risk assessment of these individuals.
Highly Reducible Nanostructured CeO2 for CO Oxidation
Authors: Feng Gang , Han Weining , Wang Zhimiao , Li Fang , Xue Wei
Published: November 2018

Ceria in nanoscale with different morphologies, rod, tube and cube, were prepared through a hydrothermal process. The structure, morphology and textural properties were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and isothermal N2 adsorption-desorption. Ceria with different morphologies were evaluated as catalysts for CO oxidation. CeO2 nanorods showed superior activity to the others. When space velocity was 12,000 mL·gcat−1·h−1, the reaction temperature for 90% CO conversion (T90) was 228 °C. The main reason for the high activity was the existence of large amounts of easily reducible oxygen species, with a reduction temperature of 217 °C on the surface of CeO2 nanorods. Another cause was their relatively large surface area.
Influence of Sulfur-Containing Sodium Salt Poisoned V2O5–WO3/TiO2 Catalysts on SO2–SO3 Conversion and NO Removal
Authors: Xiao Haiping , Dou Chaozong , Shi Hao , Ge Jinlin , Cai Li
Published: November 2018

A series of poisoned catalysts with various forms and contents of sodium salts (Na2SO4 and Na2S2O7) were prepared using the wet impregnation method. The influence of sodium salts poisoned catalysts on SO2 oxidation and NO reduction was investigated. The chemical and physical features of the catalysts were characterized via NH3-temperature programmed desorption (NH3-TPD), H2-temperature programmed reduction (H2-TPR), X-ray photoelectron spectroscopy (XPS), Brunauer–Emmett–Teller (BET), X-ray diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FT-IR). The results showed that sodium salts poisoned catalysts led to a decrease in the denitration efficiency. The 3.6% Na2SO4 poisoned catalyst was the most severely deactivated with denitration efficiency of only 50.97% at 350 °C. The introduction of SO42− and S2O72− created new Brønsted acid sites, which facilitated the adsorption of NH3 and NO reduction. The sodium salts poisoned catalysts significantly increased the conversion of SO2–SO3. 3.6%Na2S2O7 poisoned catalyst had the strongest effect on SO2 oxidation and the catalyst achieved a maximum SO2–SO3-conversion of 1.44% at 410 °C. Characterization results showed sodium salts poisoned catalysts consumed the active ingredient and lowered the V4+/V5+ ratio, which suppressed catalytic performance. However, they increased the content of chemically adsorbed oxygen and the strength of V5+=O bonds, which promoted SO2 oxidation.
Mechanistic Study on Facet-Dependent Deposition of Metal Nanoparticles on Decahedral-Shaped Anatase Titania Photocatalyst Particles
Authors: Kobayashi Kenta , Takashima Mai , Takase Mai , Ohtani Bunsho
Published: November 2018

Facet-selective gold or platinum-nanoparticle deposition on decahedral-shaped anatase titania particles (DAPs) exposing {001} and {101} facets via photodeposition (PD) from metal-complex sources was reexamined using DAPs prepared with gas-phase reaction of titanium (IV) chloride and oxygen by quantitatively evaluating the area deposition density on {001} and {101} and comparing with the results of deposition from colloidal metal particles in the dark (CDD) or under photoirradiation (CDL). The observed facet selectivity, more or less {101} preferable, depended mainly on pH of the reaction suspensions and was almost non-selective at low pH regardless of the deposition method, PD or CDL, and the metal-source materials. Based on the results, the present authors propose that facet selectivity is attributable to surface charges (zeta potential) depending on the kind of facets, {001} and {101}, and pH of the reaction mixture and that this concept can explain the observed facet selectivity and possibly the reported facet selectivity without taking into account facet-selective reaction of photoexcited electrons and positive holes on {101} and {001} facets, respectively.
Matrix Metalloproteinase-1 and Acid Phosphatase in the Degradation of the Lamina Propria of Eruptive Pathway of Rat Molars
Authors: De Pizzol Júnior José Paulo , Sasso-Cerri Estela , Cerri Paulo Sérgio
Published: November 2018

The comprehension of dental pathogenesis and disorders derived from eruption failure requires a deep understanding of the molecular mechanisms underlying normal tooth eruption. As intense remodelling is needed during tooth eruption, we hypothesize that matrix metalloproteinase-1 (MMP-1) and acid phosphatase (ACP) play a role in the eruptive pathway degradation. We evaluated MMP-1-immunoexpression and the collagen content in the lamina propria at different eruptive phases. Immunohistochemistry and ultrastructural cytochemistry for detection of ACP were also performed. In the maxillary sections containing first molars of 9-, 11-, 13-, and 16-day-old rats, the birefringent collagen of eruptive pathway was quantified. MMP-1 and ACP-2 immunohistochemical reactions were performed and the number of MMP-1-immunolabelled cells was computed. Data were analyzed by one-way ANOVA and Tukey post-test (p ≤ 0.05). ACP cytochemistry was evaluated in specimens incubated in sodium β-glycerophosphate. In the eruptive pathway of 13- and 16-day-old rats, the number of MMP-1-immunolabelled cells increased concomitantly to reduction of collagen in the lamina propria. Enhanced ACP-2-immunolabelling was observed in the lamina propria of 13- and 16-day-old rats. Fibroblasts and macrophages showed lysosomes and vacuoles containing fragmented material reactive to ACP. MMP-1 degrades extracellular matrix, including collagen fibers, being responsible for the reduction in the collagen content during tooth eruption. The enhanced ACP activity at the mucosal penetration stage indicates that this enzyme plays a role in the degradation of remnant material, which is engulfed by macrophages and fibroblasts of the eruptive pathway. Therefore, enzymatic failure in the eruptive pathway may disturbs tooth eruption.
Molecular Basis of Aquaporin-7 Permeability Regulation by pH
Authors: Mósca Andreia F. , De Almeida Andreia , Wragg Darren , Martins Ana P. , Sabir Farzana , Leoni Stefano , Moura Teresa F. , Prista Catarina , Casini Angela , Soveral Graça
Published: November 2018

The aquaglyceroporin AQP7, a family member of aquaporin membrane channels, facilitates the permeation of water and glycerol through cell membranes and is crucial for body lipid and energy homeostasis. Regulation of glycerol permeability via AQP7 is considered a promising therapeutic strategy towards fat-related metabolic complications. Here, we used a yeast aqy-null strain for heterologous expression and functional analysis of human AQP7 and investigated its regulation by pH. Using a combination of in vitro and in silico approaches, we found that AQP7 changes from fully permeable to virtually closed at acidic pH, and that Tyr135 and His165 facing the extracellular environment are crucial residues for channel permeability. Moreover, instead of reducing the pore size, the protonation of key residues changes AQP7’s protein surface electrostatic charges, which, in turn, may decrease glycerol’s binding affinity to the pore, resulting in decreased permeability. In addition, since some pH-sensitive residues are located at the monomer-monomer interface, decreased permeability may result from cooperativity between AQP7’s monomers. Considering the importance of glycerol permeation via AQP7 in multiple pathophysiological conditions, this mechanism of hAQP7 pH-regulation may help the design of selective modulators targeting aquaglyceroporin-related disorders.
Hedgehog Signaling in Cancer: A Prospective Therapeutic Target for Eradicating Cancer Stem Cells
Authors: Sari Ita Novita , Phi Lan Thi Hanh , Jun Nayoung , Wijaya Yoseph Toni , Lee Sanghyun , Kwon Hyog Young
Published: November 2018

The Hedgehog (Hh) pathway is a signaling cascade that plays a crucial role in many fundamental processes, including embryonic development and tissue homeostasis. Moreover, emerging evidence has suggested that aberrant activation of Hh is associated with neoplastic transformations, malignant tumors, and drug resistance of a multitude of cancers. At the molecular level, it has been shown that Hh signaling drives the progression of cancers by regulating cancer cell proliferation, malignancy, metastasis, and the expansion of cancer stem cells (CSCs). Thus, a comprehensive understanding of Hh signaling during tumorigenesis and development of chemoresistance is necessary in order to identify potential therapeutic strategies to target various human cancers and their relapse. In this review, we discuss the molecular basis of the Hh signaling pathway and its abnormal activation in several types of human cancers. We also highlight the clinical development of Hh signaling inhibitors for cancer therapy as well as CSC-targeted therapy.
Systemic Hypertension Effects on the Ciliary Body and Iris. An Immunofluorescence Study with Aquaporin 1, Aquaporin 4, and Na+, K+ ATPase in Hypertensive Rats
Authors: González-Marrero Ibrahim , Hernández-Abad Luis G. , Carmona-Calero Emilia M. , Castañeyra-Ruiz Leandro , Abreu-Reyes José A. , Castañeyra-Perdomo Agustín
Published: November 2018

Aquaporin 1 (AQP1) and aquaporin 4 (AQP4) have been identified in the eye as playing an essential role in the formation of the aqueous humor along with the Na+/K+ ATPase pump. Different authors have described the relationship between blood pressure, aqueous humor production, and intraocular pressure with different conclusions, with some authors supporting a positive correlation between blood pressure and intraocular pressure while others disagree. The aim of this work was to study the effect of high blood pressure on the proteins involved in the production of aqueous humor in the ciliary body (CB) and iris. For this purpose, we used the eyes of spontaneously hypertensive rats (SHR) and their control Wistar-Kyoto rats (WKY). Immunofluorescence was performed in different eye structures to analyze the effects of hypertension in the expression of AQP1, AQP4, and the Na+/K+ ATPase α1 and α2 subunits. The results showed an increase in AQP1 and Na+/K+ ATPase α1 and a decrease in AQP4 and Na+/K+ ATPase α2 in the CB of SHR, while an increase in AQP4 and no significant differences in AQP1 were found in the iris. Therefore, systemic hypertension produced changes in the proteins implicated in the movement of water in the CB and iris that could influence the production rate of aqueous humor, which would be affected depending on the duration of systemic hypertension.
Childhood Obesity and Academic Outcomes in Young Adulthood
Authors: Ryabov Igor
Published: November 2018

The present study used nationally representative data from the National Longitudinal Study of Adolescent Health (a.k.a., Add Health) to examine the impact of childhood obesity on young adult educational attainment. In addition to weight status, independent variables included race–ethnicity, immigrant generational status, family socio-economic status (SES), preference for overweight and obese friends in school, school socio-economic and race–ethnic composition, and other important predictors. Educational attainment was measured as a categorical variable with the categories reflecting key educational benchmarks: (1) being a high school graduate; (2) having some college education; and (3) having completed a bachelor’s or higher degree. The results indicate that in general, individuals who were obese as children are less likely to transition from high school to college, and even less likely to obtain a baccalaureate or more advanced degree. In line with the social network hypothesis of the obesity epidemic, we also found that having overweight and obese friends drives down the odds of educational success. Attendance at a higher SES school or a school with a lower percentage of minority students was positively associated with the odds of college attendance and obtaining a baccalaureate. Other important effects included race–ethnicity and immigrant generational status.
A Rapid Surface-Enhanced Raman Scattering (SERS) Method for Pb2+ Detection Using L-Cysteine-Modified Ag-Coated Au Nanoparticles with Core–Shell Nanostructure
Authors: Xu Zhou , Zhang Linwei , Mei Bo , Tu Jia , Wang Rong , Chen Maolong , Cheng Yunhui
Published: November 2018

A rapid surface-enhanced Raman scattering (SERS) method for Pb2+ detection has been developed based on l-cysteine-modified Ag-coated Au nanoparticles with core-shell nanostructure. Specifically, l-cysteine-functionalized Au@Ag core-shell probes bearing Raman-labeling molecules (4-ATP) are used to detect Pb2+ upon the formation of nanoparticle aggregates. The proposed SERS-based method shows a linear range between 5 pM and 10 nM, with an unprecedented limit of detection (LOD) of 1 pM for Pb2+; this LOD shows the method to be a few orders of magnitude more sensitive than the typical colorimetric approach that is based on the aggregation of noble metal nanoparticles. Real water samples diluted with pure water have been successfully analyzed. This SERS-based assay may provide a general and simple approach for the detection of other metal ions of interest, and so could have wide-ranging applications in many areas.
Increase in Efficiency of End Milling of Titanium Alloys Due to Tools with Multilayered Composite Nano-Structured Zr-ZrN-(Zr,Al)N and Zr-ZrN-(Zr,Cr,Al)N Coatings
Authors: Vereschaka Alexey , Oganyan Maksim , Bublikov Yuri , Sitnikov Nikolay , Deev Konstantin , Pupchin Vladimir , Mokritskii Boris
Published: November 2018

The study deals with an increase in the tool life parameter for metal-cutting tools and efficiency of end milling for titanium alloys, due to the use of tools with multilayered composite nano-structured Zr–ZrN–(Zr,Al)N and Zr–ZrN–(Zr,Cr,Al)N coatings, deposited through the technology of the filtered cathodic vacuum arc deposition (FCVAD). The studies included the microstructured investigations using SEM, the analysis of chemical composition (Energy-dispersive X-ray spectroscopy, EDXS), the determination of the value of critical failure force (with the use of scratch testing), and the measurement of the microhardness of the coatings under study. The cutting tests were conducted in end milling of titanium alloys at various cutting speeds. The mechanisms of wear and failure for end milling cutters with the coatings under study were studied in milling. The studies determined the advantages of using a tool with the coatings under study compared to an uncoated tool, as well as to tools with the commercial Ti–TiN coating and the nano-structured Ti–TiN–(Ti,Al)N coating. Adding Cr to the composition of the coating can significantly increase the hardness, while the coating retains sufficient ductility and brittle fracture resistance, which allows for a best result when milling titanium alloys.
Toward Superhydrophobic/Superoleophilic Materials for Separation of Oil/Water Mixtures and Water-in-Oil Emulsions Using Phase Inversion Methods
Authors: Wang Chih-Feng , Tsai Yi-Jung , Kuo Shiao-Wei , Lee Kuo-Jung , Hu Chien-Chieh , Lai Juin-Yih
Published: November 2018

In this study, a method that can simultaneously separate oil/water mixtures and water-in-oil emulsions were developed. Various substrates (synthetic polymers and stainless steel meshes) were coated by rough hydrophobic polymer films. The prepared materials possessed superhydrophobicity and superoleophilicity. These superhydrophobic sponges can isolate extensive amounts of oil from water when connected to a related vacuum framework. Moreover, the superhydrophobic meshes (SHM) can separate both surfactant-free and -stabilized water-in-oil emulsions via gravity with high separation efficiency (oil purity: >99.99%) and flux (up to 4760 L m−2 h−1). The extraordinary performance of our materials and their low-energy, efficient, low-cost preparation propose that they have great potential for real-time applications.
Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid
Authors: Kanematsu Hideyuki , Oizumi Atsuya , Sato Takaya , Kamijo Toshio , Honma Saika , Barry Dana , Hirai Nobumitsu , Ogawa Akiko , Kogo Takeshi , Kuroda Daisuke , Sano Katsuhiko , Tsunashima Katsuhiko , Lee Seung-Hyo , Lee Myeong-Hoon
Published: November 2018

N,N-diethyl-N-(2-methancryloylethy)-N-methylammonium bis(trifluoromethylsulfonyl) imide polymer (DEMM-TFSI) brush coated specimens (substrate: glasses) and a liquid ion type of polymer brush coating were investigated for their antifouling effect on biofilms. Biofilms were produced by two kinds of bacteria, E. coli and S. epidermidis. They were formed on specimens immersed into wells (of 12-well plates) that were filled with culture liquids and bacteria. The biofilm formation was observed. Also, brush coated specimens and glass substrates were investigated in the same way. DEMM polymer brush coated specimens formed more biofilm than PMMA (polymethyl methacrylate) polymer brush coated specimens and glass substrates. A greater amount of polarized components of biofilms was also observed for DEMM polymer brush coated specimens. The polar characteristics could be attributed to the attraction capability of bacteria and biofilms on DEMM polymer brush coated specimens. When considering the ease of removing biofilms by washing it with water, the ionic liquid type polymer brush (coated specimens) could be used for antifouling applications. If an initial antifouling application is needed, then the polar characteristics could be adjusted (design of the components and concentrations of ionic liquids, etc.) to solve the problem.
Effect of Self-Generated Transfer Layer on the Tribological Properties of PTFE Composites Sliding against Steel
Authors: Xie Ting , Feng Shihao , Qi Yongheng , Cui Ailong
Published: November 2018

Coatings are normally employed to meet some functional requirements. There is a kind of self-generated coating during use, such as the transfer layer during sliding, which may greatly affect the tribological behavior. Although the transfer layer has aroused much attention recently, the formation of the transfer layer closely depends on the service conditions, which need to be further studied. In this paper, the effects of sliding speed, normal load, and duration of wear test on the transfer layer thickness during friction of Ni/PTFE (Polytetrafluoroethylene) composites were experimentally investigated. The formation mechanism of transfer layer and the relationships between tribological properties and transfer layer thickness were analyzed in detail. It was found that the transfer layer thickness increased with increases of sliding speed and normal load; and after a period of wear test, the transfer layer thickness remained stable. The transfer layer thickness correlates linearly with the friction coefficient and wear volume of the PTFE composites. With the increase of the transfer layer thickness, the friction coefficient decreased, while the wear volume increased, which means that a uniform, thin, and stable transfer layer is beneficial for the reduction of friction and wear of the polymeric composites.
Electrostatic Supercapacitors by Atomic Layer Deposition on Nanoporous Anodic Alumina Templates for Environmentally Sustainable Energy Storage
Authors: Fernández-Menéndez Luis Javier , González Ana Silvia , Vega Víctor , De la Prida Víctor Manuel
Published: November 2018

In this work, the entire manufacturing process of electrostatic supercapacitors using the atomic layer deposition (ALD) technique combined with the employment of nanoporous anodic alumina templates as starting substrates is reported. The structure of a usual electrostatic capacitor, which comprises a top conductor electrode/the insulating dielectric layer/and bottom conductor electrode (C/D/C), has been reduced to nanoscale size by depositing layer by layer the required materials over patterned nanoporous anodic alumina membranes (NAAMs) by employing the ALD technique. A thin layer of aluminum-doped zinc oxide, with 3 nm in thickness, is used as both the top and bottom electrodes’ material. Two dielectric materials were tested; on the one hand, a triple-layer made by a successive combination of 3 nm each layers of silicon dioxide/titanium dioxide/silicon dioxide and on the other hand, a simple layer of alumina, both with 9 nm in total thickness. The electrical properties of these capacitors are studied, such as the impedance and capacitance dependences on the AC frequency regime (up to 10 MHz) or capacitance (180 nF/cm2) on the DC regime. High breakdown voltage values of 60 V along with low leakage currents (0.4 μA/cm2) are also measured from DC charge/discharge RC circuits to determine the main features of the capacitors behavior integrated in a real circuit.
Effect of Amplitude on the Surface Dilational Visco-Elasticity of Protein Solutions
Authors: Kovalchuk Volodymyr I. , Aksenenko Eugene V. , Trukhin Dmytro V. , Makievski Alexander V. , Fainerman Valentin B. , Miller Reinhard
Published: November 2018

Harmonic drop surface area oscillations are performed at a fixed frequency (0.1 Hz) to measure the dilational visco-elasticity for three proteins: β-casein (BCS), β-lactoglobulin (BLG), and human serum albumin (HSA). The surface area oscillations were performed with different amplitudes in order to find the origin of non-linearity effects. The analysis of data shows that the non-linearity in the equation of state—i.e., the relation between surface pressure and surface concentration of adsorbed protein molecules—is the main source of the amplitude effects on the apparent visco-elasticity, while perturbations due to non-uniform expansions and compressions of the surface layer, inertia effects leading to deviations of the drop profile from the Laplacian shape, or convective transport in the drop bulk are of less importance. While for the globular proteins, HSA and BLG the amplitude effects on the apparent visco-elasticity are rather large, for the non-globular protein BCS this effect is negligible in the studied range of up to 10% area deformation.
Anomalous Interfacial Structuring of a Non-Halogenated Ionic Liquid: Effect of Substrate and Temperature
Authors: Radiom Milad , Pedraz Patricia , Pilkington Georgia , Rohlmann Patrick , Glavatskih Sergei , Rutland Mark W.
Published: November 2018

We investigate the interfacial properties of the non-halogenated ionic liquid (IL), trihexyl(tetradecyl)phosphonium bis(mandelato)borate, [P6,6,6,14][BMB], in proximity to solid surfaces, by means of surface force measurement. The system consists of sharp atomic force microscopy (AFM) tips interacting with solid surfaces of mica, silica, and gold. We find that the force response has a monotonic form, from which a characteristic steric decay length can be extracted. The decay length is comparable with the size of the ions, suggesting that a layer is formed on the surface, but that it is diffuse. The long alkyl chains of the cation, the large size of the anion, as well as crowding of the cations at the surface of negatively charged mica, are all factors which are likely to oppose the interfacial stratification which has, hitherto, been considered a characteristic of ionic liquids. The variation in the decay length also reveals differences in the layer composition at different surfaces, which can be related to their surface charge. This, in turn, allows the conclusion that silica has a low surface charge in this aprotic ionic liquid. Furthermore, the effect of temperature has been investigated. Elevating the temperature to 40 °C causes negligible changes in the interaction. At 80 °C and 120 °C, we observe a layering artefact which precludes further analysis, and we present the underlying instrumental origin of this rather universal artefact.