bluuebunny/crossref_metadata_2025 huggingface dataset:
Determination of Quality of Life of Individuals Before and After Bariatric Surgery: Prospective Study with 1 Year Follow-Up
Authors: YILMAZ EKER Pınar , YILMAZ Meryem , TOPAL HANÇER Ayşe
Published: November 2021

Objective: This study was conducted to investigate the effect of bariatric surgery on quality of life. Methods: This study used a quantitative, descriptive, cross-sectional and prospective study design. This study was conducted between 1 January 2016 and 1 June 2017 at an university hospital in Turkey. The questionnaire form that developed by the researchers and the Short Form- 36 quality of life scale before bariatric surgery were applied to the individuals. Participants were evaluated with the same scale at 1 month, 3 months and 1 year after bariatric surgery. Results: The mean age of the individuals was 37.37 ± 9.18.While the mean of body mass index was 48.18±5.8 kg/m2 before bariatric surgery, it decreased to 27.18±3.21 kg/m2 in one year after bariatric surgery. Simple linear regression analysis was given between the subscale of SF-36 and the descriptive characteristics of the participants according to the follow-up times and there was a moderate positive relationship between preoperative physical health and gender (β = 0.450, p
Pharmacopoeia Researches and Antimicrobial Activity Studies on Matricaria chamomilla L.
Authors: ÖZDEMİR Meryem , SÜZGEÇ SELÇUK Sevda , MATARACI KARA Emel , ÖZBEK ÇELİK Berna
Published: November 2021

ABSTRACT

Objective: The aim of this study is to compare Matricaria chamomilla samples collected from nature and supplied from pharmacies and herbalists by performing quality control analyses and investigate the antimicrobial activities of them.

Methods: These samples were examined according to Matricariae flos monograph analysis in European Pharmacopoeia. Macroscopic and microscopic examination, loss on drying and total ash experiment were performed. Essential oil was obtained from the drugs and analyzed by thin layer chromatography. Additionally, aqueous and ethanolic extracts of all samples were compared by thin layer chromatography. Antimicrobial activity was determined by microdilution method in aqueous and ethanolic extracts.

Results: According to the pharmacopoeia analysis; in macroscopic and microscopic examinations, all samples except 2 herbalist samples were found to suitable for the pharmacopoeia standards. In loss on drying, none of the samples were found to suitable for the standards except 2 herbalist samples. In total ash, all samples were suitable for the standards. While the essential oil of M. chamomilla samples collected from nature was yellow-orange colored, we obtained the blue essential oil containing chamazulene from pharmacy samples and 3 of herbalist samples. The highest activity in antimicrobial activity assay was observed in ethanol extracts against Staphylococcus aureus, Staphylococcus epidermidis and Candida tropicalis strains.

Conclusion: Examples of true M. chamomilla were found among herbalist samples, contrary to the previous studies. Pharmacy samples were qualified but limited in terms of variety. Essential oil analyses pointed out the importance of geographical location among natural samples. Antimicrobial activity tests did not provide significant results.
Thoracic Oncologic Surgery in Covid-19 Pandemic: Short Term Outcomes
Authors: BİLGİ Zeynep
Published: November 2021

Objective: After sustained community spread of Coronavirus Disease – 19 (COVID-19), guidelines regarding safe practice are still evolving. In this article, we evaluated our clinic’s oncologic thoracic surgery practice in COVID-19 pandemic via an observational retrospective study.

Methods: All cases (including local anesthesia only), were recorded in the hospital electronic medical record system. Patients undergoing procedures for cancer related causes (resection, diagnosis, palliation, treatment facilitation) were retrospectively identified. Patient demographic data, type of operation/procedure, 30 day SARS-COV-2 PCR test data, hospital readmission due to COVID-19, 30 and 90 day mortality were gathered. Group 1 and 2 were operated between 1 June 2019 – 11 March 2020 and 12 March 2020 – 31 December 2020, respectively.

Results: 344 (Group 1= 178, Group 2= 166) patients underwent thoracic surgery due to cancer related causes during the study period. Surgical or COVID-19 related mortality were not encountered in either group. After initiation of mandatory testing, 3 asymptomatic patients were found to be PCR positive and had surgery rescheduled (2 lung resections, 1 mediastinoscopy). Number of lung resection cases increased post- COVID-19 (25 vs 45, p
A Review of the Use of UHPFRC in Bridge Rehabilitation and New Construction in Switzerland
Authors: Bertola Numa , Schiltz Philippe , Denarié Emmanuel , Brühwiler Eugen
Published: November 2021

Ultra-High-Performance Fibre Reinforced Cementitious Composite (UHPFRC) provides solutions to enhance existing structures and design innovative new structures. Structural UHPFRC offers 3–5 times higher compressive and tensile strengths than ordinary concrete. Due to its strain-hardening behavior and dense matrix, structures made of UHPFRC remain crack-free and waterproof, guaranteeing durability. UHPFRC has been used particularly in Switzerland with more than 280 applications since 2003. A review of UHPFRC applications in the country is proposed in this paper. Ten bridge case studies are presented, including five strengthening of existing structures and five new designs. These structures were chosen to assess the multiple benefits that UHPFRC provides compared to traditional reinforced-concrete structures. Besides structural efficiency, several construction criteria are considered, such as construction costs, material durability, environmental impacts, and construction time. Structural rehabilitation made with UHPFRC leads to cost-effective interventions, and this material also helps to preserve heritage structures. Due to its specific mechanical properties, UHPFRC enables new structures with distinctive aesthetic designs with reduced construction time. The crucial contribution of research to the first case studies is also highlighted. This link between Swiss universities and the construction industry has quickly transitioned UHPFRC Technology from academic studies to real-world applications. Nowadays, the UHPFRC Technology is maturing and applications are common in the country.
Cation-Chloride Cotransporters, Na/K Pump, and Channels in Cell Water and Ion Regulation: In silico and Experimental Studies of the U937 Cells Under Stopping the Pump and During Regulatory Volume Decrease
Authors: Yurinskaya Valentina E. , Vereninov Alexey A.
Published: November 2021

Cation-coupled chloride cotransporters play a key role in generating the Cl– electrochemical gradient on the cell membrane, which is important for regulation of many cellular processes. However, a quantitative analysis of the interplay between numerous membrane transporters and channels in maintaining cell ionic homeostasis is still undeveloped. Here, we demonstrate a recently developed approach on how to predict cell ionic homeostasis dynamics when stopping the sodium pump in human lymphoid cells U937. The results demonstrate the reliability of the approach and provide the first quantitative description of unidirectional monovalent ion fluxes through the plasma membrane of an animal cell, considering all the main types of cation-coupled chloride cotransporters operating in a system with the sodium pump and electroconductive K+, Na+, and Cl– channels. The same approach was used to study ionic and water balance changes associated with regulatory volume decrease (RVD), a well-known cellular response underlying the adaptation of animal cells to a hypoosmolar environment. A computational analysis of cell as an electrochemical system demonstrates that RVD may happen without any changes in the properties of membrane transporters and channels due to time-dependent changes in electrochemical ion gradients. The proposed approach is applicable when studying truly active regulatory processes mediated by the intracellular signaling network. The developed software can be useful for calculation of the balance of the unidirectional fluxes of monovalent ions across the cell membrane of various cells under various conditions.
Caffeine Induces Autophagy and Apoptosis in Auditory Hair Cells via the SGK1/HIF-1α Pathway
Authors: Tang Xiaomin , Sun Yuxuan , Xu Chenyu , Guo Xiaotao , Sun Jiaqiang , Pan Chunchen , Sun Jingwu
Published: November 2021

Caffeine is being increasingly used in daily life, such as in drinks, cosmetics, and medicine. Caffeine is known as a mild stimulant of the central nervous system, which is also closely related to neurologic disease. However, it is unknown whether caffeine causes hearing loss, and there is great interest in determining the effect of caffeine in cochlear hair cells. First, we explored the difference in auditory brainstem response (ABR), organ of Corti, stria vascularis, and spiral ganglion neurons between the control and caffeine-treated groups of C57BL/6 mice. RNA sequencing was conducted to profile mRNA expression differences in the cochlea of control and caffeine-treated mice. A CCK-8 assay was used to evaluate the approximate concentration of caffeine. Flow cytometry, TUNEL assay, immunocytochemistry, qRT-PCR, and Western blotting were performed to detect the effects of SGK1 in HEI-OC1 cells and basilar membranes. In vivo research showed that 120 mg/ kg caffeine injection caused hearing loss by damaging the organ of Corti, stria vascularis, and spiral ganglion neurons. RNA-seq results suggested that SGK1 might play a vital role in ototoxicity. To confirm our observations in vitro, we used the HEI-OC1 cell line, a cochlear hair cell-like cell line, to investigate the role of caffeine in hearing loss. The results of flow cytometry, TUNEL assay, immunocytochemistry, qRT-PCR, and Western blotting showed that caffeine caused autophagy and apoptosis via SGK1 pathway. We verified the interaction between SGK1 and HIF-1α by co-IP. To confirm the role of SGK1 and HIF-1α, GSK650394 was used as an inhibitor of SGK1 and CoCl2 was used as an inducer of HIF-1α. Western blot analysis suggested that GSK650394 and CoCl2 relieved the caffeine-induced apoptosis and autophagy. Together, these results indicated that caffeine induces autophagy and apoptosis in auditory hair cells via the SGK1/HIF-1α pathway, suggesting that caffeine may cause hearing loss. Additionally, our findings provided new insights into ototoxic drugs, demonstrating that SGK1 and its downstream pathways may be potential therapeutic targets for hearing research at the molecular level.
Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
Authors: Rasha Elsayim , Alkhulaifi Manal M. , AlOthman Monerah , Khalid Ibrahim , Doaa Elnagar , Alaa Khatab , Awad Manal A. , Abdalla Mohnad
Published: November 2021

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.
Mate Preference Plasticity in a Critically Endangered Frog: Implications for Conservation Breeding
Authors: Kelleher Shannon R. , Silla Aimee J. , Hertel Anne G. , Dingemanse Niels J. , Byrne Phillip G.
Published: November 2021

Variation in female mate preferences for male traits remains poorly understood (both among and within females), despite having important evolutionary and conservation implications, particularly for captive breeding. Here, we investigate female mate preferences for male advertisement call frequency, and determine whether preferences vary over repeated trials, in the critically endangered southern corroboree frog,Pseudophryne corroboree. We conducted a series of phonotaxis trials in a six-speaker arena where naïve, captive-bred, virgin females were offered a choice between low, average and high frequency male advertisement calls, with a subset of females tested repeatedly. In the first trial, we found no evidence for a population-level preference for call frequency, but females spent less time in the low call zone than expected by chance. However, our results showed that female mate preferences changed over sequential trials. Females spent significantly more time in the low frequency call zone in the third trial compared to the first trial, and, in the last trial, females exhibited a significant population-level preference for low frequency calls. Subsequently, repeatability estimates of female preferences were low and did not significantly deviate from zero. Our results indicate that femaleP. corroboreemate preferences can exhibit temporal variation, and suggest that females are more attracted to low call frequencies after repeated exposure. These findings imply that femaleP. corroboreemay become choosier over time, and highlight the potential for mate preferences to exhibit phenotypic plasticity within a single reproductive cycle. Overall, these findings provide the first information on mate preferences inP. corroboree, and emphasize the importance of considering individual variation in mate choice studies. From a conservation perspective, knowledge of individual variation in female mate preferences may be used to conduct behavioral manipulations in captivity that facilitate the breeding of genetically valuable individuals, and improve the success of conservation breeding programs.
Mass Exchange of Water and Soil on the Soil Surface in the Rainfall Splash Erosion
Authors: Sanxiang Sun , Yunxia Zhang , Pengshui Lei
Published: November 2021

This research aims to unfold the mass exchange mechanism of water and soil on the soil surface in the rainfall splash erosion process. We regard the rainfall splash erosion process as a collision process between the raindrop and the soil particle on the soil interface. This recognition allows us to incorporate research approaches from the spring vibrator model, which has been developed for simulating the impact of liquid drops on solid surface. We further argue that because a same set of factors determine the splash amount and infiltration amount and it is relatively simpler to observe the infiltration amount, an investigation into the relationship between the splash amount and infiltration amount would be able to provide a new channel for quantifying the splash erosion. This recognition leads us to examining the relationship between single raindrop, rainfall kinetic energy and splash erosion from both theoretical and empirical angles, with an emphasis on the relationship between the infiltration amount and the splash erosion. Such an investigation would add value to the collective effort to establish mass exchange law in water-soil interface during rainfall splash erosion. It is found that during the rainfall splash process, the splash erosion is proportional to the rainfall kinetic energy; and has a linear relation to the infiltration amount, with the rainfall intensity as one of important parameters and the slope depending on the unit conversation of the infiltration amount and the splash erosion. If the units of two items are same, the slope is the ratio of the soil and water density, and the splash erosion velocity of the rainfall is half of the rainfall terminal velocity. The single raindrop kinetic energy and the splash erosion have a quadratic parabola relation, and the splash velocity is about 1/3 of single raindrop terminal velocity.
Experimental Investigation on the Crack Evolution of Marine Shale with Different Soaking Fluids
Authors: Wang Lei , Bi Zhenhui , Zeng Yijin , Yang Guangguo , Guo Yintong , Yang Hanzhi
Published: November 2021

Hydration induced cracks could promote the complexity of hydraulic fractures in marine shale gas reservoir. But the evolution process and forming mechanism has not been fully investigated. In this paper, Longmaxi marine shale were collected and immersed in three types of fluids (distilled water, fracturing fluid, and mineral oil) for more than 10 days. The spatial-temporal evolution of soaking fractures was recorded and analyzed. A fracture mechanical model was established, considering the effects of in-situ stress, fluid pressure, hydration stress, and capillary force. The promotion mechanism of hydration cracks in forming complex fracking network was discussed. Results showed that hydration fractures were extremely developed and evenly distributed in a state of network for specimens immersed in distilled water. For specimens soaked in fracturing fluid, the hydration cracks were moderately developed for the addition of anti-swelling agent. Fractures were rarely developed for specimens treated in mineral oil. The hydration fractures were mainly formed in the first 5 h and showed strong anisotropy. Cracks parallel to the bedding planes accounted for the vast majority, with a small proportion developed in vertical direction. Theoretical calculations indicated that the stress intensity factor (SIF) caused by hydration stress and capillary force was greater than the measured fracture toughness. The micro crack would probably propagate along bedding planes and grow up into macro horizontal fractures, which promoted the formation of crisscrossing fracture network in shale gas formation.
Modelling Cyberspace to Determine Cybersecurity Training Requirements
Authors: Venables Adrian
Published: November 2021

Cyberspace is a constantly evolving and expanding environment that is being used for an ever-increasing range of purposes. As such, it attracts numerous threat actors seeking to identify and exploit its vulnerabilities. In order to be able to fully mitigate the risk of compromise, it is necessary to first understand the nature and composition of cyberspace and how it is used. This chapter seeks to address this issue by presenting a method to model cyberspace in three dimensions with humans included as an integral part. Expanding beyond describing cyberspace purely in terms of technology and its uses, it explores geographic, political, and temporal aspects to reflect its dynamic nature. The first component of the model examines the varied attributes of cyberspace ranging from the landscape in which its components are located to how they are used. The second dimension investigates the path of data in all its forms from its source to destination, emphasising that cyberspace is fundamentally a communications medium and is not borderless. Thirdly, it focuses on the security dimension and the motivations of those with malicious intent, demonstrating the multidisciplinary and essentially human nature of cybersecurity in countering their activities.
Reversion Mosaicism in Primary Immunodeficiency Diseases
Authors: Miyazawa Hanae , Wada Taizo
Published: November 2021

Reversion mosaicism has been reported in an increasing number of genetic disorders including primary immunodeficiency diseases. Several mechanisms can mediate somatic reversion of inherited mutations. Back mutations restore wild-type sequences, whereas second-site mutations result in compensatory changes. In addition, intragenic recombination, chromosomal deletions, and copy-neutral loss of heterozygosity have been demonstrated in mosaic individuals. Revertant cells that have regained wild-type function may be associated with milder disease phenotypes in some immunodeficient patients with reversion mosaicism. Revertant cells can also be responsible for immune dysregulation. Studies identifying a large variety of genetic changes in the same individual further support a frequent occurrence of reversion mosaicism in primary immunodeficiency diseases. This phenomenon also provides unique opportunities to evaluate the biological effects of restored gene expression in different cell lineages. In this paper, we review the recent findings of reversion mosaicism in primary immunodeficiency diseases and discuss its clinical implications.
Influenza Neuraminidase Characteristics and Potential as a Vaccine Target
Authors: Creytens Sarah , Pascha Mirte N. , Ballegeer Marlies , Saelens Xavier , de Haan Cornelis A. M.
Published: November 2021

Neuraminidase of influenza A and B viruses plays a critical role in the virus life cycle and is an important target of the host immune system. Here, we highlight the current understanding of influenza neuraminidase structure, function, antigenicity, immunogenicity, and immune protective potential. Neuraminidase inhibiting antibodies have been recognized as correlates of protection against disease caused by natural or experimental influenza A virus infection in humans. In the past years, we have witnessed an increasing interest in the use of influenza neuraminidase to improve the protective potential of currently used influenza vaccines. A number of well-characterized influenza neuraminidase-specific monoclonal antibodies have been described recently, most of which can protect in experimental challenge models by inhibiting the neuraminidase activity or by Fc receptor-dependent mechanisms. The relative instability of the neuraminidase poses a challenge for protein-based antigen design. We critically review the different solutions that have been proposed to solve this problem, ranging from the inclusion of stabilizing heterologous tetramerizing zippers to the introduction of inter-protomer stabilizing mutations. Computationally engineered neuraminidase antigens have been generated that offer broad, within subtype protection in animal challenge models. We also provide an overview of modern vaccine technology platforms that are compatible with the induction of robust neuraminidase-specific immune responses. In the near future, we will likely see the implementation of influenza vaccines that confront the influenza virus with a double punch: targeting both the hemagglutinin and the neuraminidase.
High Prevalence of Genital Human Papillomavirus Infection in Patients With Primary Immunodeficiencies
Authors: Gernert Michael , Kiesel Matthias , Fröhlich Matthias , Renner Regina , Strunz Patrick-Pascal , Portegys Jan , Tony Hans-Peter , Schmalzing Marc , Schwaneck Eva Christina
Published: November 2021

Background

Genital human papillomavirus (HPV)-infections are common in the general population and are responsible for relevant numbers of epithelial malignancies. Much data on the HPV-prevalence is available for secondary immunodeficiencies, especially for patients with human immunodeficiency virus (HIV)-infection. Little is known about the genital HPV-prevalence in patients with primary immunodeficiencies (PIDs).

Methods

We performed a cross-sectional study of patients with PIDs and took genital swabs from male and female patients, which were analyzed with polymerase chain reaction for the presence of HPV-DNA. Clinical and laboratory data was collected to identify risk factors.

Results

28 PID patients were included in this study. 10 of 28 (35.7%) had HPV-DNA in their genital swabs. 6 patients had high-risk HPV-types (21.4%). Most patients had asymptomatic HPV-infections, as genital warts were rare (2 of 28 patients) and HPV-associated malignancy was absent. Differences in the HPV-positivity regarding clinical PID-diagnosis, duration of PID, age, sex, immunosuppression, immunoglobulin replacement, or circumcision in males were not present. HPV-positive PID patients had higher numbers of T cells (CD3+), of cytotoxic T cells (CD3+/CD8+), of transitional B cells (CD19+/CD38++/CD10+/IgD+), and of plasmablasts (CD19+/CD38+/CD27++/IgD-) compared to HPV-negative.

Conclusion

PID patients exhibit a high rate of genital HPV-infections with a high rate of high-risk HPV-types. Regular screening for symptomatic genital HPV-infection and HPV-associated malignancy in PID patients seems recommendable.
Association of Microvasculature and Macular Sensitivity in Idiopathic Macular Epiretinal Membrane: Using OCT Angiography and Microperimetry
Authors: Feng Jingyang , Yang Xiaotong , Xu Mengqiao , Wang Yuwei , Shi Xiang , Zhang Yumeng , Huang Peirong
Published: November 2021

Purpose: To investigate the correlation between retinal capillary structure and macular function in patients with idiopathic epiretinal membrane (iERM) by using optical coherence tomography angiography (OCTA) and microperimetry.

Methods: This retrospective and observational study included 30 idiopathic ERM eyes of 30 consecutive patients. OCTA was performed to evaluate macular microvasculature including the superficial capillary plexus, deep capillary plexus, and foveal avascular zone. Best corrected visual acuity (BCVA) and microperimetry were measured at baseline and 3 months after surgery. Associations between macular microvasculature and visual function were assessed.

Results: Visual function including BCVA and macular sensitivity improved significantly at 3 months post-operatively (p < 0.001). At baseline, BCVA was positively correlated with foveal or parafoveal sensitivities and negatively correlated with central foveal thickness (p < 0.05). Pre-operative foveal sensitivity was significantly correlated with the vessel density of foveal or parafoveal superficial capillary plexus (p < 0.05). A multiple regression model revealed that pre-operative vessel density of foveal deep capillary plexus was an independent positive prognostic factor for post-operative BCVA (B = −0.020 ± 0.006, p = 0.006) and macular sensitivity (B = 0.200 ± 0.081, p = 0.027).

Conclusion: Integrated evaluation of iERM by using OCTA and microperimetry shows an association between microvasculature and macular sensitivity. Pre-operative vessel density of foveal deep capillary plexus assessed by OCTA may be a potentially valuable prognostic factor for iERM surgery.
Clinical Significance and Prognostic Value of Human Soluble Resistance-Related Calcium-Binding Protein: A Pan-Cancer Analysis
Authors: Zhang Jinguo , Chen Jian , Shan Benjie , Lin Lin , Dong Jie , Sun Qingqing , Zhou Qiong , Han Xinghua
Published: November 2021

The soluble resistance-related calcium-binding protein (sorcin, SRI) serves as the calcium-binding protein for the regulation of calcium homeostasis and multidrug resistance. Although the mounting evidence suggests a crucial role of SRI in the chemotherapeutic resistance of certain types of tumors, insights into pan-cancer analysis of SRI are unavailable. Therefore, this study aimed to probe the multifaceted properties of SRI across the 33 cancer types. The SRI expression was analyzed via The Cancer Genome Atlas (TCGA) and Genotype Tissue-Expression (GTEX) database. The SRI genomic alterations and drug sensitivity analysis were performed based on the cBioPortal and the CellMiner database. Furthermore, the correlations among the SRI expression and survival outcomes, clinical features, stemness, tumor mutation burden (TMB), microsatellite instability (MSI), and immune cells infiltration were analyzed using TCGA data. The differential analysis showed that SRI was upregulated in 25 tumor types compared with the normal tissues. Aberrant expression of SRI was able to predict survival in different cancers. Further, the most frequent alteration of SRI genomic was amplification. Moreover, the aberrant SRI expression was related to stemness score, epithelial-mesenchymal-transition (EMT)-related genes, MSI, TMB, and tumor immune microenvironment in various types of cancer. TIMER database mining further found that the SRI expression was significantly correlated with the infiltration levels of various immune cells in certain types of cancer. Intriguingly, the SRI expression was negatively correlated with drug sensitivity of fluorouracil, paclitaxel, docetaxel, and isotretinoin. Our findings highlight the predictive value of SRI in cancer and provide insights for illustrating the role of SRI in tumorigenesis and drug resistance.
5 Years of Exercise Intervention Did Not Benefit Cognition Compared to the Physical Activity Guidelines in Older Adults, but Higher Cardiorespiratory Fitness Did. A Generation 100 Substudy
Authors: Sokołowski Daniel R. , Hansen Tor I. , Rise Henning H. , Reitlo Line S. , Wisløff Ulrik , Stensvold Dorthe , Håberg Asta K.
Published: November 2021

Background: Aerobic exercise is proposed to attenuate cognitive decline in aging. We investigated the effect of different aerobic exercise interventions and cardiorespiratory fitness (CRF) upon cognition throughout a 5-year exercise intervention in older adults.

Methods: 106 older adults (52 women, age 70-77 years) were randomized into high-intensity interval training (HIIT; ∼90% peak heart rate), moderate-intensity continuous training (MICT; ∼70% peak heart rate), or control for 5 years. The HIIT and MICT groups performed supervised training twice weekly, while the control group was asked to follow the national physical activity guidelines (30 min of physical activity/day). At baseline, 1-, 3-, and 5-year follow-up, participants partook in cognitive testing (spatial memory, verbal memory, pattern separation, processing speed, working memory, and planning ability), underwent clinical testing, and filled out health-related questionnaires. Linear mixed models were used to assess the effects of the exercise group and CRF (measured as peak and max oxygen uptake) on each cognitive test. The effects of changes in CRF on changes in each cognitive test score throughout the intervention were also assessed. The associations between baseline CRF and cognitive abilities at the follow-ups were investigated using linear regressions.

Results: There was no group-by-time interaction on the cognitive measures, and neither HIIT nor MICT participation was associated with better cognitive performance than control at any time point during the 5-year intervention. All groups increased their CRF similarly during the 1st year and subsequently declined back to baseline levels after 5 years. A higher CRF was associated with higher processing speed throughout the intervention while increasing CRF during the intervention was associated with better working memory and worse pattern separation. Higher CRF at baseline predicted consistently better processing speed and verbal memory performance.

Conclusion: In this first 5-year randomized controlled trial investigating the effects of HIIT, MICT, and physical activity according to national guidelines on cognition, we observed no effect of exercise intervention group on cognition when compared to following the national physical activity guidelines. Still, the results showed that higher CRF and increasing CRF benefited multiple, but not all, cognitive abilities in older adults.

Clinical Trial Registration:www.ClinicalTrials.gov, identifier [NCT01666340].
An fNIRS Investigation of Discrete and Continuous Cognitive Demands During Dual-Task Walking in Young Adults
Authors: Rahman Tabassum Tahmina , Polskaia Nadia , St-Amant Gabrielle , Salzman Talia , Vallejo Diana Tobón , Lajoie Yves , Fraser Sarah Anne
Published: November 2021

Introduction: Dual-task studies have demonstrated that walking is attention-demanding for younger adults. However, numerous studies have attributed this to task type rather than the amount of required to accomplish the task. This study examined four tasks: two discrete (i.e., short intervals of attention) and two continuous (i.e., sustained attention) to determine whether greater attentional demands result in greater dual-task costs due to an overloaded processing capacity.

Methods: Nineteen young adults (21.5 ± 3.6 years, 13 females) completed simple reaction time (SRT) and go/no-go (GNG) discrete cognitive tasks and n-back (NBK) and double number sequence (DNS) continuous cognitive tasks with or without self-paced walking. Prefrontal cerebral hemodynamics were measured using functional near-infrared spectroscopy (fNIRS) and performance was measured using response time, accuracy, and gait speed.

Results: Repeated measures ANOVAs revealed decreased accuracy with increasing cognitive demands (p = 0.001) and increased dual-task accuracy costs (p < 0.001). Response times were faster during the single compared to dual-tasks during the SRT (p = 0.005) and NBK (p = 0.004). DNS gait speed was also slower in the dual compared to single task (p < 0.001). Neural findings revealed marginally significant interactions between dual-task walking and walking alone in the DNS (p = 0.06) and dual -task walking compared to the NBK cognitive task alone (p = 0.05).

Conclusion: Neural findings suggest a trend towards increased PFC activation during continuous tasks. Cognitive and motor measures revealed worse performance during the discrete compared to continuous tasks. Future studies should consider examining different attentional demands of motor tasks.
Neurosensory Prosthetics: An Integral Neuromodulation Part of Bioelectronic Device
Authors: Ezeokafor Ifeoma , Upadhya Archana , Shetty Saritha
Published: November 2021

Bioelectronic medicines (BEMs) constitute a branch of bioelectronic devices (BEDs), which are a class of therapeutics that combine neuroscience with molecular biology, immunology, and engineering technologies. Thus, BEMs are the culmination of thought processes of scientists of varied fields and herald a new era in the treatment of chronic diseases. BEMs work on the principle of neuromodulation of nerve stimulation. Examples of BEMs based on neuromodulation are those that modify neural circuits through deep brain stimulation, vagal nerve stimulation, spinal nerve stimulation, and retinal and auditory implants. BEDs may also serve as diagnostic tools by mimicking human sensory systems. Two examples of in vitro BEDs used as diagnostic agents in biomedical applications based on in vivo neurosensory circuits are the bioelectronic nose and bioelectronic tongue. The review discusses the ever-growing application of BEDs to a wide variety of health conditions and practices to improve the quality of life.
A Scatter-and-Gather Spiking Convolutional Neural Network on a Reconfigurable Neuromorphic Hardware
Authors: Zou Chenglong , Cui Xiaoxin , Kuang Yisong , Liu Kefei , Wang Yuan , Wang Xinan , Huang Ru
Published: November 2021

Artificial neural networks (ANNs), like convolutional neural networks (CNNs), have achieved the state-of-the-art results for many machine learning tasks. However, inference with large-scale full-precision CNNs must cause substantial energy consumption and memory occupation, which seriously hinders their deployment on mobile and embedded systems. Highly inspired from biological brain, spiking neural networks (SNNs) are emerging as new solutions because of natural superiority in brain-like learning and great energy efficiency with event-driven communication and computation. Nevertheless, training a deep SNN remains a main challenge and there is usually a big accuracy gap between ANNs and SNNs. In this paper, we introduce a hardware-friendly conversion algorithm called “scatter-and-gather” to convert quantized ANNs to lossless SNNs, where neurons are connected with ternary {−1,0,1} synaptic weights. Each spiking neuron is stateless and more like original McCulloch and Pitts model, because it fires at most one spike and need be reset at each time step. Furthermore, we develop an incremental mapping framework to demonstrate efficient network deployments on a reconfigurable neuromorphic chip. Experimental results show our spiking LeNet on MNIST and VGG-Net on CIFAR-10 datasetobtain 99.37% and 91.91% classification accuracy, respectively. Besides, the presented mapping algorithm manages network deployment on our neuromorphic chip with maximum resource efficiency and excellent flexibility. Our four-spike LeNet and VGG-Net on chip can achieve respective real-time inference speed of 0.38 ms/image, 3.24 ms/image, and an average power consumption of 0.28 mJ/image and 2.3 mJ/image at 0.9 V, 252 MHz, which is nearly two orders of magnitude more efficient than traditional GPUs.
Research Progress on the Ability of Astragaloside IV to Protect the Brain Against Ischemia-Reperfusion Injury
Authors: Kang Xianhui , Su Shuyue , Hong Wandong , Geng Wujun , Tang Hongli
Published: November 2021

Stroke, a disease with a sudden onset and high morbidity and mortality rates, is difficult to treat in the clinic. Traditional Chinese medicine has become increasingly widely used in clinical practice. Modern pharmacological studies have found that Radix Astragali has a variety of medicinal properties, i.e., immunoregulatory, antioxidative, anti-cancer, anti-diabetes, myocardial protective, hepatoprotective, and antiviral functions. This article reviews the protective effect and mechanism of astragaloside IV, which is extracted from Radix Astragali, on stroke, discusses the cerebroprotective effect of astragaloside IV against ischemia-reperfusion-related complications, offers insight into research prospects, and expands the idea of integrating traditional Chinese and Western medicine treatment strategies and drugs to provide a theoretical reference for the clinical treatment of cerebral ischemia-reperfusion injury and the improvement of stroke prognosis.
Voxel-Wise Adversarial FiboNet for 3D Cerebrovascular Segmentation on Magnetic Resonance Angiography Images
Authors: Guo Bin , Zhou Fugen , Liu Bo , Bai Xiangzhi
Published: November 2021

Cerebrovascular segmentation is important in various clinical applications, such as surgical planning and computer-aided diagnosis. In order to achieve high segmentation performance, three challenging problems should be taken into consideration: (1) large variations in vascular anatomies and voxel intensities; (2) severe class imbalance between foreground and background voxels; (3) image noise with different magnitudes. Limited accuracy was achieved without considering these challenges in deep learning-based methods for cerebrovascular segmentation. To overcome the limitations, we propose an end-to-end adversarial model called FiboNet-VANGAN. Specifically, our contributions can be summarized as follows: (1) to relieve the first problem mentioned above, a discriminator is proposed to regularize for voxel-wise distribution consistency between the segmentation results and the ground truth; (2) to mitigate the problem of class imbalance, we propose to use the addition of cross-entropy and Dice coefficient as the loss function of the generator. Focal loss is utilized as the loss function of the discriminator; (3) a new feature connection is proposed, based on which a generator called FiboNet is built. By incorporating Dice coefficient in the training of FiboNet, noise robustness can be improved by a large margin. We evaluate our method on a healthy magnetic resonance angiography (MRA) dataset to validate its effectiveness. A brain atrophy MRA dataset is also collected to test the performance of each method on abnormal cases. Results show that the three problems in cerebrovascular segmentation mentioned above can be alleviated and high segmentation accuracy can be achieved on both datasets using our method.
Effect of Aqueous Enzymatic Extraction of Deer Oil on Its Components and Its Protective Effect on Gastric Mucosa Injury
Authors: Xia Yun-Shi , Sun Yin-shi , Liu Chang , Li Zhi-Man , Ren Duo-Duo , Mu Rui , Zhang Yan-Ting , Bo Pan-Pan , Zhao Li-juan , Wang Zi
Published: November 2021

In this study, deer suet fat was used as a raw material to study the effects of aqueous enzymatic extraction of deer oil on its components, followed by studies into the potential protective activity, and related molecular mechanisms of deer oil on ethanol-induced acute gastric mucosal injury in rats. The results show that aqueous enzymatic extraction of deer oil not only has a high extraction yield and has a small effect on the content of active ingredients. Deer oil can reduce total stomach injury. Without affecting the blood lipid level, it can reduce the oxidative stress, which is manifested by reducing the content of myeloperoxidase (MPO) and enhancing the activity level of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). It also enhances the expression of defense factors prostaglandin (E2), epidermal growth factor (EGF), and somatostatin (SS), it inhibits apoptosis evidenced by the enhanced of Bcl-2 and decreased expression of cleavage of caspase-3 and Bax. At the same time, it reduces inflammation, which is manifested by reducing the expression of IL-1β, interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) gastric tissue pro-inflammatory cytokines, and enhancing the expression of anti-inflammatory factors IL-4 and IL-10, and inhibiting the mitogen-activated protein kinase/nuclear factor kappa B (MAPK/NF-κB) signaling pathway in gastric tissue.
Identification of the Unique Clinical and Genetic Features of Chinese Lung Cancer Patients With EGFR Germline Mutations in a Large-Scale Retrospective Study
Authors: Lin Xinqing , Peng Muyun , Chen Quanfang , Yuan Mingming , Chen Rongrong , Deng Haiyi , Deng Jiaxi , Liu Ouqi , Weng Yuqing , Chen Mingjiu , Zhou Chengzhi
Published: November 2021

Background

Epidemiological surveys have suggested that lung cancer has inherited susceptibility and shows familial aggregation. However, the distribution and prevalence of epidermal growth factor receptor (EGFR) germline variants and their roles in lung cancer genetic predisposition in Chinese population remain to be elucidated.

Methods

In this study, EGFR germline and somatic variants were retrospectively reviewed from the next-generation sequencing results of 31,906 patients with lung cancer. Clinical information was also collected for patients with confirmed EGFR germline mutations.

Results

A total of 22 germline EGFR variants were identified in 64 patients with lung cancer, accounting for 0.2% of the total cases studied. Five patients were diagnosed as multiple primary carcinomas. Family history was documented in 31.3% (20/64) of patients, 55% of which were diagnosed as lung cancer. G863D was the most frequent EGFR germline mutation, followed by P848L, D1014N, and K757R. Somatic EGFR-sensitive mutations were identified in 51.6% of patients with germline EGFR mutations. The proportion of L858R mutation, exon 19 deletion, and rare sensitive mutation was 50%, 17.6%, and 32.4%, respectively. D1014N and T790M mutations were common in young patients. The family members of patients with P848L, R776H, V769M, and V774M mutations were more commonly diagnosed with cancers. A total of 19 patients were confirmed to have received EGFR tyrosine kinase inhibitors (TKIs), but the response to EGFR-TKIs differed among patients with different EGFR mutations.

Conclusion

Chinese patients with lung cancer harbored unique and dispersive EGFR germline mutations and showed unique clinical and genetic characteristics, with varied response patterns to EGFR-TKI treatment.
Clinical Characteristics and Outcomes of Children With Extracorporeal Membrane Oxygenation in a Developing Country: An 11-Year Single-Center Experience
Authors: Iamwat Wirapatra , Samankatiwat Piya , Lertbunrian Rojjanee , Anantasit Nattachai
Published: November 2021

Introduction: Extracorporeal Membrane Oxygenation (ECMO) is a lifesaving procedure for patients with refractory cardiac or respiratory failure. The indications for ECMO are growing, and it is increasingly being used to support cardiopulmonary failure in children. However, the risks and benefits of ECMO should be weighed before deploying it on the patients. The objectives of this study were to identify the mortality risk factors and to determine the ECMO outcomes.

Methods: The retrospective chart reviews were done for all patients aged 1 day−20 years old receiving ECMO between January 2010 and December 2020.

Results: Seventy patients were enrolled in the study. The median age was 31.3 months. The incidence of VA and VV ECMO was 85.7 and 14.3%, respectively. The most common indication for ECMO was the failure to wean off cardiopulmonary bypass after cardiac surgery. Pre-existing acute kidney injury (OR 4.23; 95% CI 1.34–13.32, p = 0.014) and delayed enteral feeding (OR 3.85, 95% CI 1.23–12.02, p = 0.020), and coagulopathy (OD 12.64; 95% CI 1.13–141.13, p = 0.039) were associated with the higher rate of mortality. The rates of ECMO survival and survival to discharge were 70 and 50%, respectively.

Conclusion: ECMO is the lifesaving tool for critically ill pediatric patients. Pre-existing acute kidney injury, delayed enteral feeding, and coagulopathy were the potential risk factors associated with poor outcomes in children receiving ECMO. However, ECMO setup can be done successfully in a developing country.
Extracorporeal Shockwave Lithotripsy for Cystine Stones in Children: An Observational, Retrospective, Single-Center Analysis
Authors: Vinit Nicolas , Khoury Antoine , Lopez Pauline , Heidet Laurence , Botto Nathalie , Traxer Olivier , Boyer Olivia , Blanc Thomas , Lottmann Henri B.
Published: November 2021

Purpose: Cystinuria is a genetic disorder characterized by a defective reabsorption of cystine and dibasic amino acids leading to development of urinary tract calculi from childhood onward. Cystine lithiasis is known to be resistant to fragmentation. The aim was to evaluate our long-term experience with extracorporeal shockwave lithotripsy (ESWL) used as first-line urological treatment to treat cystine stones in children.

Methods: We retrospectively reviewed the charts of all children who underwent ESWL for cystine stone. We assessed the 3-month stone-free rate, according to age, younger (group 1) or older (group 2) than 2 years old.

Results: Between 2003 and 2016, 15 patients with a median (IQR) age at first treatment of 48 (15–108) months underwent ESWL in monotherapy. Median age was, respectively, 15 and 108 months in each group. The median (IQR) stone burden was 2,620 (1,202–8,265) mm3 in group I and 4,588 (2,039–5,427) mm3 in group II (p = 0.96). Eleven patients had bilateral calculi. ESWL was repeated on average 2.4 times, with a maximum of 4 for patients of group I, and 4.8 times, with a maximum of 9 for group II (p > 0.05). ESWL in monotherapy was significantly more efficient to reach stone-free status for children under 2 years of age: 83% vs. 6.2% (p = 0.040). The median (IQR) follow-up of the study was 69 (42–111) months.

Conclusion: ESWL appears as a valid urological option for the treatment of cystine stones, in young children. Even if cystine stones are known to be resistant to fragmentation, we report 83% of stone-free status at 3 months with ESWL used in monotherapy in children under 2 years old with cystinuria. In older children, the success rate is too low to recommend ESWL as a first line approach.