bluuebunny/crossref_metadata_2025 huggingface dataset:
Allosteric deactivation of PIFs and EIN3 by microproteins in light control of plant development
Authors: Wu Qingqing , Kuang Kunyan , Lyu Mohan , Zhao Yan , Li Yue , Li Jing , Pan Ying , Shi Hui , Zhong Shangwei
Published: July 2020

Significance

How plants respond and adjust their growth to cope with environmental changes is a long-standing question in biology. By studying the initial dark-to-light transition, we reveal that a microprotein-imposed suppression of protein activity is adopted by plants to achieve rapid switch from skotomorphogenic to photomorphogenic programs. Microproteins are essential bioactive regulators in animals. A large number of microproteins are predicted in plants, but very few of them are functionally characterized. We identified two microproteins that are tissue-specifically stimulated by light and directly disrupt the oligomerization of key transcription factors in light signaling. This microprotein-directed allosteric deactivation can be utilized to develop versatile tools for posttranslational regulation of target proteins in molecular breeding.
Autonomics: In search of a foundation for next-generation autonomous systems
Authors: Harel David , Marron Assaf , Sifakis Joseph
Published: July 2020

Significance

Autonomous systems are replacing humans in a variety of tasks, and in the years to come, such systems will become central and crucial to human life. They will include vehicles of all kinds, medical and industrial robots, agricultural and manufacturing facilities, traffic management systems, and much more. While many organizations strive to develop the next generation of trustworthy, cost-effective autonomous systems, a major gap exists between the challenges in developing these and the state of the art. There is a crucial need for a common scientific and engineering foundation for developing these systems, which we term “autonomics.” We believe that such a foundation will dramatically accelerate the deployment and acceptance of high-quality autonomous systems, for the benefit of human society.
Lipid sponge droplets as programmable synthetic organelles
Authors: Bhattacharya Ahanjit , Niederholtmeyer Henrike , Podolsky Kira A. , Bhattacharya Rupak , Song Jing-Jin , Brea Roberto J. , Tsai Chu-Hsien , Sinha Sunil K. , Devaraj Neal K.
Published: July 2020

Significance

Organelles spatially and temporally orchestrate biochemical reactions in a cell to a degree of precision that is still unattainable in synthetic reaction systems. Additionally, organelles such as the endoplasmic reticulum (ER) contain highly interconnected and dense membrane networks that provide large reaction spaces for both transmembrane and soluble enzymes. We present lipid sponge droplets to emulate the functions of organelles such as the ER. We demonstrate that lipid sponge droplets can be programmed to internally concentrate specific proteins, to host and accelerate biochemical transformations, and to rapidly and reversibly sequester and release proteins to control enzymatic reactions. The self-assembled and programmable nature of lipid sponge droplets will facilitate the integration of complex functions for bottom-up synthetic biology.
Metformin inhibits RAN translation through PKR pathway and mitigates disease in
C9orf72
ALS/FTD mice
Authors: Zu Tao , Guo Shu , Bardhi Olgert , Ryskamp Daniel A. , Li Jian , Khoramian Tusi Solaleh , Engelbrecht Avery , Klippel Kelena , Chakrabarty Paramita , Nguyen Lien , Golde Todd E. , Sonenberg Nahum , Ranum Laura P. W.
Published: July 2020

Significance

Repeat-associated non-AUG (RAN) proteins accumulate in patient brains and contribute to a growing number of neurodegenerative diseases. There is an urgent need to understand why expression of these proteins does not require canonical or near-cognate AUG start codons and to develop ways to block RAN protein production. We show several types of repeat-expansion RNAs activate the double-stranded RNA-dependent protein kinase (PKR) pathway and that blocking PKR reduces RAN protein levels in cells. PKR is activated in
C9orf72
ALS/FTD human and mouse brains and PKR inhibition using AAV-PKR-K296R or the FDA-approved drug metformin decreases RAN protein levels and improves disease in ALS/FTD mice. Targeting PKR using gene therapy or metformin are promising therapeutic approaches for
C9orf72
ALS/FTD and other expansion diseases.
Light modulates the gravitropic responses through organ-specific PIFs and HY5 regulation of
LAZY4
expression in
Arabidopsis
Authors: Yang Panyu , Wen Qiming , Yu Renbo , Han Xue , Deng Xing Wang , Chen Haodong
Published: July 2020

Significance

Light and gravity are two key environmental factors that coordinately regulate plant gravitropism, but the underlying molecular mechanisms that drive this regulation are poorly understood. Here, we reveal that light represses expression of
LAZY4
, a positive regulator of gravitropism, by promoting degradation of PIF proteins to inhibit negative gravitropism of hypocotyls in
Arabidopsis
. Meanwhile, light induces
LAZY4
expression by promoting HY5 accumulation to enhance positive gravitropism in roots. Our findings provide key insights regarding the mechanism by which light mediates organ-specific gravitropism in plants.
Intact proviral DNA assay analysis of large cohorts of people with HIV provides a benchmark for the frequency and composition of persistent proviral DNA
Authors: Simonetti Francesco R. , White Jennifer A. , Tumiotto Camille , Ritter Kristen D. , Cai Mian , Gandhi Rajesh T. , Deeks Steven G. , Howell Bonnie J. , Montaner Luis J. , Blankson Joel N. , Martin Albine , Laird Gregory M. , Siliciano Robert F. , Mellors John W. , Siliciano Janet D.
Published: July 2020

Significance

Despite clinically effective antiretroviral therapy (ART), replication-competent HIV-1 persists as latent proviral DNA capable of rekindling viral replication when ART is stopped. To cure the infection, therapies are being developed to eliminate latent HIV-1. Accurate assays for intact or rebound-competent HIV-1 are important to this effort. We previously developed the intact proviral DNA assay (IPDA) as a more accurate and scalable assay for intact HIV-1 proviruses. Here we present IPDA analysis in a diverse cohort of 400 ART-treated individuals. We confirm that intact proviruses, although vastly outnumbered by defective proviruses, are present at significantly higher frequencies than previously detected by viral outgrowth assays. We also show that IPDA amplicon signal issues, observed in 6.3% of samples, result from sequence polymorphisms.
Identification of GLUT12/SLC2A12 as a urate transporter that regulates the blood urate level in hyperuricemia model mice
Authors: Toyoda Yu , Takada Tappei , Miyata Hiroshi , Matsuo Hirotaka , Kassai Hidetoshi , Nakao Kazuki , Nakatochi Masahiro , Kawamura Yusuke , Shimizu Seiko , Shinomiya Nariyoshi , Ichida Kimiyoshi , Hosoyamada Makoto , Aiba Atsu , Suzuki Hiroshi
Published: July 2020

Recent genome-wide association studies have revealed some genetic loci associated with serum uric acid levels and susceptibility to gout/hyperuricemia which contain potential candidates of physiologically important urate transporters. One of these novel loci is located upstream of
SGK1
and
SLC2A12
, suggesting that variations in these genes increase the risks of hyperuricemia and gout. We herein focused on
SLC2A12
encoding a transporter, GLUT12, the physiological function of which remains unclear. As GLUT12 belongs to the same protein family as a well-recognized urate transporter GLUT9, we hypothesized that GLUT12 mediates membrane transport of urate. Therefore, we conducted functional assays and analyzed
Glut12
knockout hyperuricemia model mice, generated using the CRISPR-Cas9 system. Our results revealed that GLUT12 acts as a physiological urate transporter and its dysfunction elevates the blood urate concentration. This study provides insights into the deeper understanding of the urate regulatory system in the body, which is also important for pathophysiology of gout/hyperuricemia.
Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach
Authors: Bowman Emily K. , Deaner Matthew , Cheng Jan-Fang , Evans Robert , Oberortner Ernst , Yoshikuni Yasuo , Alper Hal S.
Published: July 2020

Significance

Traditional genetic modulation approaches are typically restricted to binary perturbations, single-sided titrations (either graded up- or down-regulation), or individual gene expression modulation. Here, we utilize a library approach that is able to simultaneously modulate gene expression in a metabolism-wide manner. This library, when coupled with next-generation sequencing, allows for the identification of gene perturbations that would have been missed by classic approaches. For the examples tested, this library identified targets that improved growth on alternative carbon sources as well as improved production of betaxanthins.
Phosphoprotein-based biomarkers as predictors for cancer therapy
Authors: Carter Angela M. , Tan Chunfeng , Pozo Karine , Telange Rahul , Molinaro Roberto , Guo Ailan , De Rosa Enrica , Martinez Jonathan O. , Zhang Shanrong , Kumar Nilesh , Takahashi Masaya , Wiederhold Thorsten , Ghayee Hans K. , Oltmann Sarah C. , Pacak Karel , Woltering Eugene A. , Hatanpaa Kimmo J. , Nwariaku Fiemu E. , Grubbs Elizabeth G. , Gill Anthony J. , Robinson Bruce , Gillardon Frank , Reddy Sushanth , Jaskula-Sztul Renata , Mobley James A. , Mukhtar M. Shahid , Tasciotti Ennio , Chen Herbert , Bibb James A.
Published: July 2020

Significance

This study validates a path toward personalized medicine that utilizes a survey of the effective signaling state of tumors via posttranslational modification analysis as a diagnostic alternative to direct examination of genetic mutations. Here, this approach is utilized to identify biomarkers for Cdk5-driven tumors. The same approach could be adapted to identify markers of other tumor drivers. As the number of cancer types in which Cdk5 is implicated is rising and as drugs with the potential to selectively target Cdk5 are moved toward the clinic, the translational potential of this diagnostic–therapeutic coupled system will be broad.
Nanoparticles presenting clusters of CD4 expose a universal vulnerability of HIV-1 by mimicking target cells
Authors: Hoffmann Magnus A. G. , Bar-On Yotam , Yang Zhi , Gristick Harry B. , Gnanapragasam Priyanthi N. P. , Vielmetter Jost , Nussenzweig Michel C. , Bjorkman Pamela J.
Published: July 2020

Significance

The ability of HIV-1 to rapidly develop resistance against various therapeutics remains a roadblock to finding a cure. Here we propose that nanoparticle-based therapeutics that mimic HIV-1 target cells by presenting clusters of CD4, the HIV-1 receptor, could prevent effective viral escape. We show that CD4 multimerization on the nanoparticle dramatically enhanced the neutralization potency and breadth compared with conventional CD4-based reagents that present only one or two copies of CD4. The CD4 nanoparticles neutralized a range of diverse HIV-1 strains, including patient isolates resistant to multiple broadly neutralizing antibodies. This work describes a new therapeutic direction that exploits the need of HIV-1 to bind CD4 on target cells, thus warranting further investigation for the potential development of a cure.
Incremental structure building of preverbal PPs in Dutch
Authors: Coopmans Cas W. , Schoenmakers Gert-Jan
Published: July 2020

Incremental comprehension of head-final constructions can reveal structural attachment preferences for ambiguous
phrases. This study investigates how temporarily ambiguous PPs are processed in Dutch verb-final constructions. In De
aannemer heeft op het dakterras bespaard/gewerkt ‘The contractor has on the roof terrace saved/worked’, the PP is
locally ambiguous between attachment as argument and as adjunct. This ambiguity is resolved by the sentence-final verb. In a
self-paced reading task, we manipulated the argument/adjunct status of the PP, and its position relative to the verb. While we
found no reading-time differences between argument and adjunct PPs, we did find that transitive verbs, for which the PP is an
argument, were read more slowly than intransitive verbs, for which the PP is an adjunct. We suggest that Dutch parsers have a
preference for adjunct attachment of preverbal PPs, and discuss our findings in terms of incremental parsing models that aim to
minimize costly reanalysis.
Morphological processing in nominalizations
Authors: Lipzig Emma van , Creemers Ava , Don Jan
Published: July 2020

A major debate in psycholinguistics concerns the representation of morphological structure in the mental lexicon. We report the results of an auditory primed lexical decision experiment in which we tested whether verbs prime their nominalizations in Dutch. We find morphological priming effects with regular nominalizations (schorsen‘suspend’ →schorsing‘suspension’) as well as with irregular nominalizations (schieten‘shoot’ →schot‘shot’). On this basis, we claim that, despite the lack of phonological identity between stem and derivation in the case of irregular nominalizations, the morphological relation between the two forms, suffices to evoke a priming effect. However, an alternative explanation, according to which the semantic relation in combination with the phonological overlap accounts for the priming effect, cannot be excluded.
Measuring SARS-CoV-2 neutralizing antibody activity using pseudotyped and chimeric viruses
Authors: Schmidt Fabian , Weisblum Yiska , Muecksch Frauke , Hoffmann Hans-Heinrich , Michailidis Eleftherios , Lorenzi Julio C.C. , Mendoza Pilar , Rutkowska Magdalena , Bednarski Eva , Gaebler Christian , Agudelo Marianna , Cho Alice , Wang Zijun , Gazumyan Anna , Cipolla Melissa , Caskey Marina , Robbiani Davide F. , Nussenzweig Michel C. , Rice Charles M. , Hatziioannou Theodora , Bieniasz Paul D.
Published: July 2020

The emergence of SARS-CoV-2 and the ensuing explosive epidemic of COVID-19 disease has generated a need for assays to rapidly and conveniently measure the antiviral activity of SARS-CoV-2–specific antibodies. Here, we describe a collection of approaches based on SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective human immunodeficiency virus type-1 (HIV-1), and vesicular stomatitis virus (VSV), as well as a replication-competent VSV/SARS-CoV-2 chimeric virus. While each surrogate virus exhibited subtle differences in the sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent plasma and human monoclonal antibodies measured using each virus correlated quantitatively with neutralizing activity measured using an authentic SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic immunity conferred by vaccination or prior SARS-CoV-2 infection, as well as the potency of convalescent plasma or human monoclonal antibodies.
3 m × 3 m heterolithic passive resonant gyroscope with cavity length stabilization
Authors: Zhang Fenglei , Liu Kui , Li Zongyang , Feng Xiaohua , Li Ke , Ye Yanxia , Sun Yunlong , He Leilei , Schreiber K Ulrich , Luo Jun , Lu Zehuang , Zhang Jie
Published: July 2020

Large-scale high sensitivity laser gyroscopes have important applications for ground-based and space-based gravitational wave detection. We report on the development of a 3 m × 3 m heterolithic passive resonant gyroscope (HUST-1) which is installed on the ground of a cave laboratory. We operate the HUST-1 on different longitudinal cavity modes and the rotation sensitivity reaches 1.6 × 10−9 rad s−1 Hz−1/2 above 1 Hz. The drift of the cavity length is one of the major sensitivity limits for our gyroscope in the low frequency regime. By locking cavity length to an ultra-stable reference laser, we achieve a cavity length stability of 5.6 × 10−9 m Hz−1/2 at 0.1 mHz, a four orders of magnitude improvement over the unconstrained cavity in the low frequency regime. We stabilize the cavity length of a large-scale heterolithic passive resonant gyroscope through active feedback and realize long-term operation. The rotation sensitivity reaches 1.7 × 10−7 rad s−1 Hz−1/2 at 0.1 mHz, a three orders of magnitude improvement over the unconstrained cavity, which is no longer limited by the cavity length drift in this frequency range.
Wavelet-aided guided wavefield imaging of delaminations in laminated composite plates
Authors: Sha Ganggang , Xu Hao , Radzieński Maciej , Cao Maosen , Ostachowicz Wiesław
Published: July 2020

Delamination is a commonly encountered type of damage in composite structures, which may lead to degraded performance and even failure of structures. Thus, detection of delaminations is considered a vital task to maintain structural integrity and reliability. This paper presents a delamination imaging method based on the analysis of guided wavefields. The method relies on the fact that delaminations in a structure will cause wavefield anomalies, which in turn can be utilized for the indication of delamination zones. Such anomalies, however, could be easily masked by the major components of the wavefield signals including incident waves from actuators and reflected waves from structural boundaries. To address the problem, one-dimensional wavelet transform (WT) is used to process the wavefields leading to WT wavefields. The WT wavefields can highlight signal anomalies associated with delaminations. A WT wavefield imaging algorithm is first constructed step by step in detecting multiple delaminations in a glass fiber reinforced polymer (GFRP) plate. The algorithm is then successfully used in the imaging of a single delamination zone in a carbon fiber reinforced polymer (CFRP) plate. The locations, sizes, and shapes of the delamination zones in the GFRP and CFRP plates are clearly visualized in the ultimate damage maps.
High-bandwidth open-loop motion control of a piezo-positioning mechanism based on a composite electromechanical dynamic model
Authors: Yang Haigen , Zhu Wei
Published: July 2020

The piezo-positioning system with high dynamic performance is urgently needed in the field of precision manufacturing and precision instrumentation. The observed rate-dependent hysteresis presents more challenges and difficulties for accurately modeling the response behavior of the piezoelectric stack actuators over the last several decades. Our previous research shows that the rate-dependent characteristics may be caused by the dynamic characteristics of the power amplifier. In order to achieve the high-bandwidth and high-speed motion control accuracy, we propose an open-loop compensation control method of a piezo-positioning mechanism based on a composite electromechanical dynamic model. The dynamic model can describe the dynamic characteristics of the power amplifier, the inverse piezoelectric effect and the dynamics of the mechanism. Experimental results show that the proposed control method can achieve precise motion control of the piezo-positioning mechanism within 1–800 Hz bandwidth.
Collagen particles with collagen-binding bone morphogenetic protein-2 promote vertebral laminar regeneration in infant rabbits
Authors: Cui Yi , Xu Bai , Yin Yanyun , Chen Bing , Zhao Yannan , Xiao Zhifeng , Yang Bin , Shi Ya , Fang Yongxiang , Ma Xu , Dai Jianwu
Published: July 2020

The vertebral laminar defects caused by severe spina bifida occulta, spinal fracture, or bone tuberculosis require surgical treatment. The reconstruction of vertebral laminar defects remains challenging, especially in children. In this study, we created an animal model of vertebral laminar defects in newly weaned rabbits to evaluate the therapeutic effect of bovine bone collagen particle (BBCP) that combined with bone morphogenetic protein-2 with collagen binding domain (CBD-BMP-2). The tissues at the injury site which were harvested after 12 weeks indicated that newly formed bone was observed in both BBCP and BBCP/CBD-BMP-2 groups, whereas the injury site of the control group was mostly filled by fibrous tissue. The BBCP/CBD-BMP-2 group recovered better than the BBCP group. These findings indicate that a combination of BBCP with CBD-BMP-2 may be a good strategy for vertebral laminar defects in children.
Whole Effluent Toxicity (WET) Test On Microalgae Reactor and Acute Toxicity on Daphnia sp.
Authors: Bayu Kartikasari Ika , Yulianto A , Asmarany A
Published: July 2020

Processing using alga reactor was made one of the solutions in wastewater treatment. Symbiotic relationship of Algal-bacteria increases the potency of processing wastewater. These capabilities can be enhanced by compounding CO2 in photosynthesis in processing wastewater. WET (Whole Effluent Toxicity) testing is needed to monitor the effects of toxicity of algae reactor. The research was carried out using waste of Water Waste Management Installations in Sewon, Yogyakarta with supplying 0.2 L/min and 0.5 L/min of CO2. This research aims to know the LC50 and analyze the characteristic relationship of waste water as the results of algae reactor process. Acute toxicity test is performed with a static method within 24 hours using animal test Daphnia sp. on day 1, 6 and 13. Based on the analysis of the characteristics of waste are known that DO, pH, temperature, light intensity strongly influenced the death of Daphnia sp. Based on the results of the analysis of algae-bacteria, the best result came with the addition of CO2 amounting to 0.2 L/min. While the worst occurred at reactor Algae.
Structural design of airport runway Case study: Jos Orno Imsula MOA Airport
Authors: Khoemarga Kelly V , Noor Tajudin Anissa
Published: July 2020

Jos Orno Imsula MOA Airport is a SATPEL class airport. This airport is a potential services airport. Jos Orno Imsula MOA airport has an existing runway with the dimensions of 1400 m x 30 m, with azimuth direction 10 – 28, and 60 cm pavement thickness. This study aim to redesign the geometric and pavement thickness of runway. The methods used in this study are Aeroplane Reference Field Length (ARFL) method for calculating the length of the runway, and Federal Aviation Administration (FAA) method for calculating the pavement thickness of runway. The analysis shows that the dimension of runway required is 1260 x 30 m, and the pavement thickess required is 45 cm.
Benefits of the Opex Pro Application in Online Project Monitoring and Evaluation at PT. XYZ
Authors: Ronald Simanjuntak Manlian A. , Nahdi Mustafa
Published: July 2020

Along with the process of globalization, information technology has become an inseparable part of the world of construction services. However, the use of information technology in the field of construction management is not as popular as compared to construction planning especially in the process of project monitoring and evaluation. The contractor must have an integrated information system to be able to support the process of monitoring and evaluation that is true and right so that objectives can be achieved according to plan. PT. XYZ is a contractor that creates an integrated software-based information system called Opex Pro that is used to support project monitoring and evaluation. Opex Pro is used on smartphones and has been implemented for a number of years, but the benefits and positive effects have yet to be measured. This study aims to identify components and analyze the benefits of implementing the Opex Pro application as a means of project monitoring and evaluation. The scope of the problems in this study is limited to the application of the Opex Pro Application in the Building Division of PT. XYZ with a sample project manager. Literature review is focused on construction management, information technology in the world of construction, project monitoring and evaluation, and management of construction resources. The quantitative approach was chosen as the research method and analysis was carried out on all selected samples. It is expected that the application of the Opex Pro application can have a positive impact on the project monitoring and evaluation process at PT. XYZ continuously.
Designing Press Tool For Carton Finishing Process To Improve Productivity And Efficiency
Authors: Widodo Lamto , Adianto  , Rohana Nasution Siti , Wijaya Priadi
Published: July 2020

Using cardboard as packaging protects the product from damage while at the same time improving its appearance to attract the attention of customers. The packaging company, PT. XYZ, works manually without using machines or tools. As a result, inefficiencies tend to occur because workers waste time and energy. It also has the potential to cause extreme fatigue and workplace accidents. This research is a field of ergonomics research, the aim is to understand the physical complaints of workers, the work sequence of workers, to then design a helper tool and finally evaluate the work posture of workers, before and after repairs. To improve workers ‘conditions, researchers collected workers’ identities and analyzed their physical complaints using questionnaires, including Nordic Body Map, REBA analysis, OWAS analysis, MFA analysis, and measurement of right and left hand maps in standard time. We also identified workers’ needs, initial specifications to conceptualize the design of assistive devices. We obtain the design concept by determining the final specifications and checking the data using CATIA software. The final REBA score after applying the helper press yields a score of 3 on pressing and folding cartons. Improvements also managed to eliminate lifting and lowering activities during the finishing process. Analysis result of the working conditions before and after implementation are as follows; cycle time decreased from 320.23 to 275 seconds (14.12%), normal time decreased from 336.24 to 299.75 seconds (11%), and standard time dropped from 437.11 to 374.69 seconds (14, 3%).
Land mapping with least median of squares regression using landsat imagery: a case study Jakarta and sorrounding area
Authors: Mulyawan Bagus , Jovianto Nicholas , Hendryli Janson , Erny Herwindiati Dyah
Published: July 2020

Impervious surface is an area that has undergone natural or semi-natural substitution of artificial land cover which is usually waterproof and relatively permanent, including settlements, industrial buildings, road networks, railroad networks, high voltage electricity networks, airports air and sea ports. Changes cause the narrowing of rivers and open land which become water catchment areas. One of the problems that triggered an increase in impervious land is urbanization. This study aims to map land changes in the Jakarta and surrounding areas using the least median of squares regression method. Based on data from land classification results using 4 classes, Jakarta and Tangerang are areas that have more impervious land than non-impervious land. Meanwhile, Bogor, Bekasi, and Depok are areas that have more non-impervious land
Evaluation of Side Friction in Ihcm for Highway 4 Lanes 2 Ways Divided
Authors: Najid
Published: July 2020

IHCM (Indonesia Highway Capacity Manual) was issued and came into force in 1997. After 20 years there has been a change in traffic from the number and composition, as well as the traffic regulation policy. As a result of this, the determination of IHCM’s road capacity is often incorrect. Therefore it is necessary to evaluate IHCM. This study tries to evaluate IHCM through side friction factors, as one of the factors that determine the value of road capacity. Research is limited to evaluating the weight of each side friction factor and the effect of land use from the survey results using statistical analysis. From the analysis, it is found that the conditions for the use of shops, office and campus are in the category of high and low of side friction factor and the ranking of the relative weights of the side friction factors in IHCM is quite different.
Socket prosthesis manufacturing process made from bamboo fiber composite materials
Authors: Purna Irawan Agustinus , Wayan Sukania I , Anggarina Paula T. , Raditya Danendra Albertus , Dimas Baskara Gregorius
Published: July 2020

This research aims to develop the manufacturing process of making socket prosthesis with bamboo fiber composite material and epoxy matrix. Bamboo fiber was chosen because it is strong, environmentally friendly, and recyclable. The focus of research is to arrange the manufacturing process of making sockets, starting from material preparation to socket testing. The method used in this study is a customized method and prototype socket manufacture in accordance with the dimensions obtained from the patient. Based on the results of the research that has been carried out, stages of the manufacturing process, results of compressive strength testing, and morphological analysis of socket products are obtained. These results will be a reference in future research.
Flat Plate Type Solar Collector Performance Using Double Thermal Insulation
Authors: Setyawan EkoYohanes , Kurniawan Arif , Rahmadianto Febi , Napitupulu Richard A. M , Siagian Parulian
Published: July 2020

Indonesia is a country that has a huge potential of solar radiation energy since it is located in the equator. This causes Indonesia to have a tropical climate and receives almost the same emission of solar radiation throughout the year. National average solar energy potential is 16 MJ/day. This energy potential can be used as a source of thermal energy. To improve the energy potential, a research performance is done by using double thermal insulation solar collectors so that the energy can be received optimally. One alternative that is used is adding Styrofoam and Polyurethane which are installed on all sides of the collector except the upper side. Styrofoam and Polyurethane have 3 cm thickness and low thermal conductivity value therefore it is able to isolate the energy inside the solar collector. This experiment was done in a day with energy coming at the test reached 8,798,634 j/m2 with 69% efficiency level.
Determine The Value of Mark Up at Bid Price Hotel Buildings
Authors: Dwijayanti M , Johny J
Published: July 2020

Cost plan has an important role in construction projects. Every part of activites need cost from preliminary to hand over the project. As an owner representative to control the budget project, a quantity surveyor has to prepare an owner estimate. The owner estimate is a reference to evaluate the quotation in bidding process. The accuracy of calculation owner estimate is very important. Cost-based pricing is one of the strategies in the bidding process, the formula of quotation is cost of the project plus mark up. In the process of bidding, contractors have their consideration of internal and external factors in every project, so they produced a different value of mark up. Many experts made formula for bidding strategy, in Friedman’s and Gates’s formula the value of mark up is determined by probability of winning in every contractor. Purpose of this research is to determine the amount of mark up by Friedman’s and Gates’s formula. The result of analysis with Friedman’s and Gates’s formula is more or less at seven percent of mark up. The result is determined by number and type of competitors.
Risk Identification in Procurement of Glass facade in High-Rise Office Buildings in Jakarta
Authors: Simanjuntak Manlian Ronald A. , Tanumihardja Reinaldi
Published: July 2020

This research aimed to identify the risk of the procurement stage of glass façade in the high-rise office building in Jakarta. In this research, the procurement phase is analyzed using the PMBOK reference (5th edition). The number of risks that occur during the procurement process can certainly hamper the project. These risks can cause to the project completion time that are not in accordance with the project schedule. Besides that, the risks could make the project cost even greater. This certainly can lead to big loss to the project. Therefore, it is very important to identify the main risks at the procurement stage from the beginning of the construction process. According to the PMBOK, the stages of the procurement is divided into 4, which consists of plan procurement, conduct procurement, control procurement and close procurement. Based on the research results, obtained 45 risks that are divided into 4 phases of the procurement.
Study Phase of Operational Management Process X Building
Authors: Bari Putri Janerizka , Simanjuntak Manlian Ronald A.
Published: July 2020

Building operational management has consistently become a requirement to fulfill, especially for buildings that are enabled for the public interest. The concept of operational management especially in the maintenance of poor buildings can cause a variety of functions building facilities will be increasingly decreased and affect the quality and comfort of the building, hence it is very important to the owner of the building/Building users know the understanding and process of building operational management. This research aims to study the understanding of operational management and based on research results there are 4 stages of the process in operational management, namely review design, planning, organizing, movement, and Control.
Risk Identification in Procurement of Precast Facade Procurement on High Rise Buildings in Jakarta
Authors: Sulistianto Ardian , Ronald Dr. Manlian , Simajuntak A.
Published: July 2020

The purpose of this research is to identify the risk of the precast façade procurement of buildings in Jakarta. In this study there are three phases, procurement planning, implementation of the procurement and procurement management. The stage refers to PMBOK (6th Edition). On the procurement of precast façade wall buildings there are several constraints that can be the cause of risk that affects the delay of the construction project. Therefore, the main problem in this research is to be able to know and identify the cause of the risk of precast façade wall procurement on high rise buildings in Jakarta and to know the precast façade wall character on Building. The results of this research are expected to be a reference in preventing and minimizing the risks that occur in precast façade wall procurement in high rise buildings in Jakarta.
Evaluation And Decrease Of The Likelihood Of Risks In Mass Floor Excavations According To Your Texture In Peru
Authors: Ramos Jhannel Arteaga , Alvarez Giancarlo Andia
Published: July 2020

Safety in the area of the construction is a matter of great interest and concern for both the public and private sectors, since there is a huge number of occupational accident occurrences, many of them with unfortunate endings for the existence of lives lost, being consequence mainly by the activity of massive and manual excavations.For this reason, the use and understanding of the norms in the safety and contingency plans is fundamental when starting a project, since the rigorous monitoring of this plan will define the increase or decrease in the probability of risk that each activity may have in construction.
Comparative study between MSC Marc/Mentat student version and Simufact Welding for three-passed butt joint
Authors: Napitupulu Richard A.M. , Hutabarat Chilinton , Manurung Charles S.P , Manurung Yupiter HP , Graf Marcel
Published: July 2020

In this research, FEM simulation was created butt joint with weld filler 3D-solid geometry. The assigned substrate element type is created and modeled on a 9 mm thickness. The three-passes of butt joint was undergoing with using the MSC Marc / Mentat student version simulation and simufact welding simulation. The butt joint designed with three passes which are penetration, filler pass and the capping for both simulations. Thermo-mechanical FEM simulations using MSC Marc / Mentat student version and simufact welding are implemented throughout the analysis. The preferred heat source model is an ellipsoid Goldak normally used for arc welding processes and weld path process in one direction. The temperature distribution caused by the welding process on the specimens must be analyzed with the same clamping conditions and the same load. Both simulations were collecting the temperature distribution to observe the calibration of software. From the results of the temperature distribution, which simulation can be assessed which is more accurate. But to be more accurate in comparing it, the two simulations must be compared directly with the experimental, with the similar material, dimensions, and welding parameters.
Concept design of solar cell satellite dish
Authors: Teja Steven , Travis Adrianto Nicholas , Louis Martin JM , Kurniawan Nicholas , Purna Irawan Agustinus
Published: July 2020

Solar panels are devices that consist of solar cells that convert light into electricity. They are called solar on the Sun or “sol” because the Sun is the strongest light source that can be utilized. Solar panels are often called photovoltaic cells, photovoltaic can be interpreted as “light-electricity”. Solar cells or PV cells depend on the photovoltaic effect to absorb the Sun’s energy and cause currents to flow between two opposing charged layers. The purpose of this study is to analyze the satellite dish using solar panels, so this tool is a signal search tool that uses solar energy to run to carry out its functions. As we know that many places or areas that are still often experiencing power outages so that it can hamper and interfere with community activities. Therefore, this research wants to facilitate the public, especially the current generation who need electricity every day so that when there is a power outage, the satellite dish will utilize solar energy as its main source. This research uses a satellite dish as an intermediary to produce electrical energy with the help of solar energy and is collected into a high-voltage battery in the form of electricity, so that it becomes a source of electricity.
Project Acceleration of Outside Plant-Fiber Optic (OSP-FO) Project in PT. XYZ Using Time Cost Trade Off (TCTO) Method by Adding Overtime Hours
Authors: Nur Hasyyati Syifa , Arum Puspita Ika , Tripiawan Wawan
Published: July 2020

Various things can occur in the implementation of a construction project that could lead an increase implementation time so that the completion of the project tends to be delayed or not according to plan. The OSP-FO Project was chosen as a case study because of the 3rd license party is not released yet from 5th July 2018 until entered May, 2019. The project is not having production work for more than 6 weeks and the status of the project is become on hold project. Therefore, it is necessary to renew the schedule and accelerate the project when the license is release. Time Cost Trade Off (TCTO) method is a schedule compression to get a project that is more profitable in terms of time (duration), cost, and income. The goal is to compress projects with acceptable duration and minimize the total cost of the project by selecting critical activities with adding optimum working hours for 3 hours per day. From the results of the analysis, the optimum cost is Rp. 22,397,932,645,- and the optimum duration is 292 days, so that the cost efficiency is Rp. 91,596,790 or 0,0041% and the time efficiency is 78 days or 0.2110%.
Lean Manufacturing Implementation on Extrude Process with Value Stream Maping: Study Case in Tyre Manufacture
Authors: Sri Ngadono Teguh , Rokhim Muh , Fitri Ikatrinasari Zulfa
Published: July 2020

Lean Manufacturing has been known by all industries as one of the strategic tools adopted to compete in the tight market competition recently. This research will be focus on implementation of lean manufacturing at the one of the biggest local tire manufactures located in Tangerang which had a problem of production achievement was 92% to the schedule planned and the main caused by shortage building due to waiting material from preparation department. Value Stream Mapping (VSM) will be used to identify the waste at extruder since 55% problems was came from extruder. The method approach by started of identifying VSM current state, Gap analysis and action plan, and the last step is identify VSM future state and review the result. From VSM Future state, its resulted the reduction of lead time process extruder from 13.72 minutes/batch to 12.8 minutes/batch and WIP stock can be reduced from 12, 8 hours to 10.6 hours. Finally the shortage at building has been reduce from 7% to 3.2% and OEE building is increasing from 61% to 64.8%.
Comparison Study of Facility Planning and Layouts Studies
Authors: Bintang Bagaskara K. , Gozali Lina , Widodo Lamto , Jusuf Daywin Frans
Published: July 2020

Facilities and layout planning is a study discipline that has take an important role in company management. There are a lot of problem and inflicts the company because of having a wrong layout of facilities. One of them are material handling in production area, cost of material handling will increase and inflicts the company just because the company has a wrong layout design. Because it takes a long time and a long distance to carry the material from the warehouse to the production area until to the finished goods warehouse. Systematic Layout Planning (SLP) can improve the effectiveness of the wrong layout, this method has other submethod that improve efficiency material flow and decrease the number of infliction, including time; distance; and money. Researcher has a result of literatures review that conclude other methods in facilities layout planning with 10 international journal.
Implementation of Statistical Quality Control to Reduce Defects in Mabell Nugget Products (Case Study at PT. Petra Sejahtera Abadi)
Authors: Alfatiyah Rini , Bastuti Sofian , Kurnia Dadang
Published: July 2020

Food and beverage industry is one of the five priority industrial sectors in the implementation of Industry 4.0 program. According to data from the ministry of industry throughout 2018, the food and beverage industry is able to grow by 7.91 percent or exceed national economic growth at 5.17 percent. PT. Petra Sejahtera Abadi is a company engaged in producing processed foods made from chicken and beef with freezing processes such as; sausage, smoke beef, spicy chick, spicy wings, nugget, rollade, and cornade beef. Data on the number of defects per year of nugget products from PT. Petra Sejahtera Abadi for the period of 2015 were 23, 453 disabled, in 2016 as many as 21, 876 disabled, in 2017 as many as 20, 987 disabled. Based on the description above, the goal to be achieved in this study is to determine the Statistical Quality Control (SQC) method with control chart techniques and cause and effect diagrams applied by the company in controlling quality to minimize failed products. The results of the SQC implementation contained data that was out of control. For those out of the upper control limit, the 4th period in August 2018 and the 6th period in October 2018 are Out of the Upper Control Line (UCL). The most influential factor in the occurrence of product defects is the machine and material factors that must be immediately corrected.
CONSUMER PREFERENCE ANALYSIS OF SNACK USING CONJOINT ANALYSIS METHOD (CASE STUDY : TELUR GABUS)
Authors: Laricha Salomon Lithrone , Kosasih Wilson , Doaly Carla O. , Cindy
Published: July 2020

Recently, the production of snacks continues to experience developments ranging from types, variants of taste, basic ingredients, and appearance. The aim of this research is to develop snack products in an effort to increase market share of the product itself. The example of the product for this research commonly known as Telur Gabus. Conjoint analysis and cluster analysis methods are used in processing the data obtained. Through the distribution of questionnaires, was obtained attributes that are considered in the development of this product that consist of texture, variant of taste, how to open the package, the weigh of contents, how to carry the packaging, and packaging design. The number of combination profiles is 16 combination profile level attributes and analyzed so that the combination of attributes with the highest utility value is chosen. The combination of attributes with the highest utility value are products that have a solid texture, have a salty taste, use zipper packaging, contents inside the packaging is 100-150 gr, carried by hand and have a packaging product design with a sticker covering half the packaging.
Aggregate and Disaggregate Production Planning, Material Requirement, and Capacity Requirement in PT. XYZ
Authors: Lefta F , Gozali L , Marie I A
Published: July 2020

Good production planning which suitable for the company in fulfilling demand will increase amount of profits. PT. XYZ is a company which produce talc powder in lots of type, such as Globe 107 sterile, Globe 107 non-sterile, Globe 207 sterile, Globe 207 non-sterile, Globe 307 sterile, Globe 307 non-sterile, Globe 407 sterile, Globe 407 non- sterile, Globe 507 sterile, and Globe 507 is non-sterile. PT. XYZ problem is they can’t fulfil all of their demands. Data used in this research are demand data from August 2015 until July 2019, company’s cost component, and capacity of each machine used in the production process. The aim of this research is to project the next 12 months demands, determine the best lot sizing technique, and determine the capacity requirement for PT. XYZ. Based on the research, the best forecasting method is using artificial neural network, the best lot sizing technique is using AWW method with total cost Rp 2.310.465.513. The capacity problems in crusher and sterile machine are solved by implementing overtime, while in jet mill, sieve screening, and packaging can reduce their working shift.
Comparative Study Between the Integration of ITIL and ISO / IEC 27001 with the Integration of COBIT and ISO / IEC 27001
Authors: Kholis Gunawan Nur , Budiarto Hadiprakoso Raden , Kabetta Herman
Published: July 2020

This paper tries to get a comparison between the integration of Information Technology Infrastructure Library (ITIL) and ISO / IEC 27001 with Integration of COBIT and ISO / IEC 27001. Factors to be considered are the increased level of information security services credibility, cost of the two standards integration of, the time of implementation, the multiple processes removal, and the increase in understanding between parts of the organization. A literature study is used as a research method. The results of the research can be a reference for organizations to consider two information security standards that will be integrated.
A brief review of attacks and mitigations on smartphone infrastructure
Authors: Setyawan Rico , Rahayu Allicia A. , Nur Annisa K. F. , Amiruddin Amiruddin
Published: July 2020

The increasing number of smartphone users will encourage competition among smartphone manufacturers in innovating and marketing their products. Such competition can create open space for attackers to understand how smartphones work and then spread threats through malicious programs. In this study, a brief review of various threats and mitigations is carried out on smartphones infrastructure, especially on several well-known brand smartphones.
Cardiac Arrhythmia classification using deep learning
Authors: Silalahi Alexander R. J. , Pandiangan Tumpal
Published: July 2020

The present work aims to obtain an automated program based on deep learning to detect and classify cardiac arrhythmia using electrocardiogram (ECG) data. One of the main obstacles in working with medical dataset is the limited availability of public medical data and the fact that only a small fraction of data represents non-normal medical conditions. In the present work we will compare the two convolutional neural network models inspired by VGGNet and ResNet in predicting of cardiac arrhythmia. Our calculations show that the two methods are equally good in predicting the overall accuracy for large dataset. However, ResNet shows a slightly better performance in predictions for much smaller dataset.