bluuebunny/crossref_metadata_2025 huggingface dataset:
Gauge-Invariant Quantum Thermodynamics: Consequences for the First Law
Authors: Céleri Lucas C. , Rudnicki Łukasz
Published: January 2024

The universality of classical thermodynamics rests on the central limit theorem, due to which, measurements of thermal fluctuations are unable to reveal detailed information regarding the microscopic structure of a macroscopic body. When small systems are considered and fluctuations become important, thermodynamic quantities can be understood in the context of classical stochastic mechanics. A fundamental assumption behind thermodynamics is therefore that of coarse graining, which stems from a substantial lack of control over all degrees of freedom. However, when quantum systems are concerned, one claims a high level of control. As a consequence, information theory plays a major role in the identification of thermodynamic functions. Here, drawing from the concept of gauge symmetry—essential in all modern physical theories—we put forward a new possible intermediate route. Working within the realm of quantum thermodynamics, we explicitly construct physically motivated gauge transformations which encode a gentle variant of coarse graining behind thermodynamics. As a first application of this new framework, we reinterpret quantum work and heat, as well as the role of quantum coherence.
Finger Vein Recognition Using DenseNet with a Channel Attention Mechanism and Hybrid Pooling
Authors: Devkota Nikesh , Kim Byung Wook
Published: January 2024

This paper proposes SE-DenseNet-HP, a novel finger vein recognition model that integrates DenseNet with a squeeze-and-excitation (SE)-based channel attention mechanism and a hybrid pooling (HP) mechanism. To distinctively separate the finger vein patterns from their background, original finger vein images are preprocessed using region-of-interest (ROI) extraction, contrast enhancement, median filtering, adaptive thresholding, and morphological operations. The preprocessed images are then fed to SE-DenseNet-HP for robust feature extraction and recognition. The DenseNet-based backbone improves information flow by enhancing feature propagation and encouraging feature reuse through feature map concatenation. The SE module utilizes a channel attention mechanism to emphasize the important features related to finger vein patterns while suppressing less important ones. HP architecture used in the transitional blocks of SE-DenseNet-HP concatenates the average pooling method with a max pooling strategy to preserve both the most discriminative and contextual information. SE-DenseNet-HP achieved recognition accuracy of 99.35% and 93.28% on the good-quality FVUSM and HKPU datasets, respectively, surpassing the performance of existing methodologies. Additionally, it demonstrated better generalization performance on the FVUSM, HKPU, UTFVP, and MMCBNU_6000 datasets, achieving remarkably low equal error rates (EERs) of 0.03%, 1.81%, 0.43%, and 1.80%, respectively.
A Multiple-Sensor Fault-Tolerant Control of a Single-Phase Pulse-Width Modulated Rectifier Based on MRAS and GPI Observers
Authors: Dardouri M. , Salman M. , Khojet El Khil S. , Boccaletti C. , Jelassi K.
Published: January 2024

Due to their advantages in ensuring low harmonic distortion and high power factors, single-phase Pulse-Width Modulated (PWM) rectifiers are widely employed in several industrial applications. Generally, the conventional control loop of a single-phase PWM rectifier uses both voltage and current sensors. Hence, in case of sensor fault, the performance and the availability of the converter can be seriously compromised. Therefore, diagnosis approaches and fault-tolerant control (FTC) strategies are mandatory to monitor these systems. Accordingly, this paper introduces a novel multiple-sensor FTC scheme for a single-phase PWM rectifier. The proposed fault diagnosis approach relies on joining several Generalized Proportional Integral (GPI) and Model Reference Adaptive System (MRAS) observers with a residual generation technique to detect and isolate sensor faults in a simple and reliable manner. While conventional sensor FTC methods dedicated to PWM rectifiers can only deal with single faults, the suggested approach guarantees a very good effectiveness level of sensor fault detection, isolation (FDI) and FTC of multiple-sensor fault occurrence scenarios. Consequently, the single-phase PWM rectifier can work with only the survivable single sensor with the guarantee of very good performance as in healthy operation mode. The effectiveness of the proposed sensor FDI approach and its control reconfiguration performance are demonstrated through both extensive simulation and experimental results.
An Assessment of the Possibility of Using Unmanned Aerial Vehicles to Identify and Map Air Pollution from Infrastructure Emissions
Authors: Jaroń Agata , Borucka Anna , Deliś Paulina , Sekrecka Aleksandra
Published: January 2024

Sustainable development and the creation of smart, green cities requires cooperation in many scientific fields, including those related to ecology, mobility, or sustainable management, among others. Environmental protection is a particularly important element here. Atmospheric pollution, due to air movements, spreads over very large areas; therefore, air quality monitoring is crucial to ensure protection from harmful substances. One of the most severe sources of air pollution, accounting for as much as approximately 25% of total annual emissions within the EU, is road transport. Therefore, the European Union has set an ambitious target to reduce total emissions to 55% for cars and 50% for vans by 2030. In recent years, unmanned aerial vehicles (UAVs) have become increasingly popular in many scientific fields, including environmental protection and photogrammetry. The use of UAVs to identify harmful pollutants allows them to gain an advantage over conventional detection methods, due to the possibility of remote (therefore safe for humans), faster, and area-based measurements. Given the ever-expanding scale of the use of this technology, this paper presents the possibilities of using UAVs to identify and visualize (map) pollution. The examples presented in the foreign literature, as well as our own research, in imaging the altitude distribution of air pollutants; gaseous pollutants: C6H6, HCHO, SO2; and particulate matter: PM1, PM2.5, PM10 demonstrate the validity of such measures. This research was carried out in the area of one of Poland’s key A4 highways. The maps obtained allow for an area-wise and altitude-wise presentation of one of the significant air pollutants in the EU. In addition, they can be a valuable source of information for the implementation of future projects and the improvement of road infrastructure, thus contributing to the reduction of air pollution and the creation of so-called “green cities”.
Thermal Characteristics and User Regulation of Household Heat Metering for Residential District Heating Systems in Northern China
Authors: Luo Ao , Li Chunyang , Xia Jianjun , Cheng Pengyue
Published: January 2024

Heat metering is an important measure of China’s heating marketization reform as well as an energy-saving policy implemented in buildings nearly 20 years ago. However, this policy has not achieved the expected results due to various reasons. It is important to note that although northern China is dominated by large apartment buildings, resulting in unique thermal characteristics and user behavior, the measured results of these characteristics are not common. In this study, data from cities in northern China were obtained using household heat meters and field testing. Based on the three levels of station, building, and household, the effects of location, heat outages, and users’ regulation behavior on energy consumption were analyzed. The results show that different locations lead to a considerable difference in heat consumption among users, and an outage leads to a significant increase in consumption by surrounding users. For typical buildings in northern China, it is challenging to realize energy savings with district heating systems based on user behavior, and household metering and charging are not fair and reliable. Adjusting the heat meter unit from the household to the building was suggested.
Fractional-Order Sliding-Mode Control and Radial Basis Function Neural Network Adaptive Damping Passivity-Based Control with Application to Modular Multilevel Converters
Authors: Yang Xuhong , Chen Wenjie , Yin Congcong , Cheng Qiming
Published: January 2024

This paper proposes a hybrid control scheme that combines fractional-order sliding-mode control (FOSMC) with radial basis function neural network adaptive damping passivity-based control (RBFPBC) for modular multilevel converters (MMC) under non-ideal operating conditions. According to the passive control theory, we establish the Euler–Lagrange (EL) models of positive and negative sequences based on the unbalanced grid. A passivity-based controller that satisfies the energy dissipation law is designed. To enable rapid convergence of the system energy storage function, a radial basis function neural network (RBFNN) is introduced to adjust the injection damping adaptively. Additionally, a fractional-order sliding-mode controller (FOSMC) is designed. The fractional-order sliding mode surface used can improve tracking performance, and effectively suppressed the undesirable chattering phenomenon compared to the traditional sliding-mode control (SMC). Finally, combining the two control methods can effectively solve the issue of passivity-based control (PBC) being too dependent on parameters. The proposed hybrid control scheme enhances the ability of the system to resist disturbances, and improves its overall robustness. Simulation results demonstrate the feasibility and effectiveness of this control method.
Physicochemical Characterization, Thermal Behavior, and Pyrolysis Kinetics of Sewage Sludge
Authors: Messaoudi Hanane , Koukouch Abdelghani , Bakhattar Ilias , Asbik Mohamed , Bonnamy Sylvie , Bennouna El Ghali , Boushaki Toufik , Sarh Brahim , Rouboa Abel
Published: January 2024

Pyrolysis is an energy recovery technique with significant potential for managing wastewater treatment plant byproducts. This research aims to investigate the physicochemical and thermal properties of Moroccan sludge, as well as the behavior of its decomposition during pyrolysis at three different heating speeds (5, 10, and 20 K/min). Characterization of the sludge before pyrolysis through ultimate analysis, proximate analysis, FTIR spectroscopy, and XRD revealed that the sludge consists predominantly of organic matter, with a volatile matter rate of 48%, an ash rate of 37%, and a higher heating value (HHV) of 15 MJ/kg. The TGA-DTG curves identified four distinct stages in the sludge decomposition process: drying, decomposition of organic matter, degradation of calcium carbonate, and decomposition of inorganic matter. Using TG-MS analysis, the principal gases identified during pyrolysis were H2O, H2, CH4, CO2, CO, NO, and SO. The average activation energies (Ea) determined through kinetics models were found to be 413.4 kJ/mol for the Kissinger–Akahira–Sunose (KAS) model, 419.6 kJ/mol for the Flynn–Wall–Ozawa (FWO) model, and 416.3 kJ/mol for the Starink model. The values of Ea and the pre-exponential coefficient (A) obtained through the KAS, FWO, and Starink techniques are consistent with ΔG values ranging between 152 and 155 KJ/mol. The positive ΔS values range from 0.003 to 1.415 kJ/mol.K, indicating the complexity of the sludge response during pyrolysis and the spontaneity of the chemical reaction at high temperatures. The kinetic data obtained serves as a pillar for the development and improvement of sewage sludge pyrolysis systems, reinforcing their role in sustainable energy production.
Protective Role of Limosilactobacillus fermentum Lf2 and Its Exopolysaccharides (EPS) in a TNBS-Induced Chronic Colitis Mouse Model
Authors: Ale Elisa C. , Irazoqui José M. , Ale Analía , Peralta Guillermo H. , Puntillo Melisa , Burns Patricia , Correa Olivar Gabriela , Cazenave Jimena , Bergamini Carina V. , Amadio Ariel F. , Binetti Ana G.
Published: January 2024

Limosilactobacillus fermentum Lf2 (Lf2) is an autochthonous strain that produces high levels of exopolysaccharides (EPS). The objective of this work was to evaluate the probiotic potential of Lf2 and its relationship with these metabolites in a mouse model of TNBS (trinitrobenzene sulfonic acid)-induced chronic colitis. Mice were treated intrarectally with increasing doses of TNBS resuspended in 50% ethanol for 14 days. In parallel, they received different treatments by gavage (lactose 10% as the matrix): freeze-dried Lf2 (L); purified EPS (E); and lactose 10% (T). A healthy control group (H) was treated with 50% alcohol without TNBS (intrarectally) and 10% lactose (by gavage). In the small intestine, there was a significant increase in IgA levels for the group that received EPS and a decrease in IFN-γ for mice treated with the strain compared to the other groups. In the large intestine, IL-2 and IFN-γ presented the lowest levels in the groups treated with EPS and the strain. The concentrations of acetic and propionic acids in mice that received Lf2 were the highest, while the levels of butyric acid were comparable to the healthy control group. An increase in the abundance of SCFA-producing bacteria was observed for mice treated with EPS and the strain in comparison with the colitis control group. The enzyme activity of catalase was higher in all the treatments compared to the TNBS-induced colitis control mice. To summarize the results obtained, a principal component analysis (PCA) was performed, clearly grouping the treatments in different clusters according to the variables studied. This is one of the first studies to address the role of a potential probiotic strain in a chronic colitis mouse model, trying to elucidate the relationship between its properties and the EPS synthesized.
Seeing Colors: A Literature Review on Colorimetric Whole-Cell Biosensors
Authors: Nemer Georgio , Koubaa Mohamed , El Chamy Laure , Maroun Richard G. , Louka Nicolas
Published: January 2024

Colorimetric whole-cell biosensors are natural or genetically engineered microorganisms utilized to detect target molecules and ions as indicators of pollutants and biological activity in the environment. Upon detection, within specific concentration ranges which vary depending on the microorganism and its genetic circuitry among other factors, these sensors produce pigments which can be detected with the human eye past certain thresholds and quantified using simple analytical techniques, namely spectrophotometry. These sensors, which can be rendered portable through lyophilization and other methods, provide valuable and reliable substitutes of more demanding analytical ex situ techniques. The insights gained from this review can highlight technological progress in the field and contribute to the identification of potential opportunities afforded by these advancements.
Experimental Estimation of Turbulent Flame Velocity in Gasoline Vapor Explosion in Multi-Branch Pipes
Authors: Lin Keyu , Zhang Peili , Duan Jimao , Xiang Shuo , Shen Ting’ao , Yang Chaoshan
Published: January 2024

The overpressure characteristics of gasoline explosions in multi-branch pipes are caused by various factors, with flame velocity as a particularly significant determinant. Overlooking the impact of turbulent flow in the branch pipes can induce a significant discrepancy in the outcome when using laminar flame velocity to determine the maximum rate of overpressure rise. To quantify the impact of turbulent flame velocity on the rate of overpressure rise in the gasoline explosions within branch pipes, the laminar flame velocity was replaced with its turbulent counterpart. Additionally, modifications to the formula for calculating the maximum overpressure rise rate were implemented. Then, experimental data of peak explosion overpressure and overpressure rise rate under different numbers of branches were obtained. Finally, the empirical data were inputted into the modified formula to determine the maximum rate of overpressure rise, thus enabling the calculation of the turbulent flame velocity across varying numbers of branches. The findings reveal a positive correlation between the number of branches and the turbulent flame velocity during tube explosions. When the number of branch pipes increased from 0 to 4, the turbulent flame velocity was found to range from 8.29 to 13.39 m/s. The increase in the number of branches did not consistently enhance the turbulent flame velocity. As the number of branches increased from zero to three, the turbulent flame velocity rose accordingly. Differently, as the number of branches exceeds three, the turbulent flame velocity exhibits fluctuations and peaks at a level approximately 1.8 times higher. The research method of this paper can provide a reference for estimating the turbulent flame velocity in the combustion process of flammable gas explosions in multi-branch tunnels.
Changes in Cooking Characteristics, Structural Properties and Bioactive Components of Wheat Flour Noodles Partially Substituted with Whole-Grain Hulled Tartary Buckwheat Flour
Authors: Zhang Mengna , Chen Zhigang
Published: January 2024

The whole-grain, hulled Tartary buckwheat flour (HTBF) with outstanding bioactive functions was prepared, and the effects of partial substitution ratios (0, 30%, 51% and 70%) of wheat flour with HTBF on the characteristics of TB noodles (TBNs) were investigated, mainly including the cooking characteristics, sensory analysis, internal structure, bioactive components, and in vitro starch digestibility. With an increasing replacement level of HTBF, the water absorption index of the noodles decreased, whereas the cooking loss increased. A sensory analysis indicated that there were no off-flavors in all TBN samples. The scanning electron microscope images presented that the wheat noodles, 30% TBNs and 70% TBNs had dense and uniform cross sections. Meanwhile, the deepest color, V-type complexes, and lowest crystallinity (13.26%) could be observed in the 70% TBNs. A HTBF substitution increased the rutin content and the total phenolic and flavonoid contents in the TBNs, and higher values were found in the 70% TBNs. Furthermore, the lowest rapidly digestible starch content (16%) and highest resistant starch content (66%) were obtained in the 70% TBNs. Results demonstrated that HTBF could be successfully applied to make TBNs, and a 70% substitution level was suggested. This study provides consumers with a good option in the realm of special noodle-type products.
Supplemental Stability Criteria with New Formulation for Linear Time-Invariant Fractional-Order Systems
Authors: Lv Yuanda , Zhang Jin-Xi , Zhang Xuefeng
Published: January 2024

In this paper, new stability criteria for linear time-invariant fractional-order systems (LTIFOSs) based on linear matrix inequalities (LMIs) are derived. The solved variable of the existing LMI formulations is generalized to a complex one. In addition, based on the congruent transformation, a new LMI formulation is obtained, which is different from those in the existing literature. To deal with the above LMIs more conveniently with simulation software, the complex LMIs are converted to equivalent real LMIs. Finally, numerical examples are presented to validate the effectiveness of our theoretical results.
Oleogels as a Promising Alternative to Animal Fat in Saturated Fat-Reduced Meat Products: A Review
Authors: Ferdaus Md. Jannatul , Barman Bishal , Mahmud Niaz , da Silva Roberta Claro
Published: January 2024

The surge in chronic diseases is closely linked to heightened levels of saturated and trans fatty acids in processed foods, particularly meat products. Addressing this concern, various strategies have been employed to alleviate the impact of these detrimental fats. Among these, oleogels have emerged as a novel and promising approach in the food industry. As restructured fat systems, oleogels offer a unique opportunity to enhance the nutritional profile of meat products while providing distinct health and environmental advantages. This comprehensive review explores the transformative role of oleogels as innovative substitutes for traditional animal fats in a variety of meat products. Utilizing materials such as hydroxypropyl methylcellulose (HPMC), sterols, beeswax, γ-oryzanol, β-sitosterol, and others, oleogels have been investigated in diverse studies. The examination encompasses their impact on the textural, nutritional, and oxidative dimensions of meat patties, pork patties, pork liver pâtés, beef heart patties, and meat batters. An in-depth exploration is undertaken into the influence of various elements, including the type of oil, gelling agents, and processing methods, on the stability and physicochemical attributes of oleogels. Additionally, the paper scrutinizes the potential effects of oleogels on sensory attributes, texture, and the shelf life of meat products. In conclusion, this collective body of research emphasizes the versatility and efficacy of oleogels as viable replacements for traditional animal fats across a spectrum of meat products. The documented improvements in nutritional quality, oxidative stability, and sensory attributes pave the way for the development of healthier and more sustainable formulations in the meat industry.
Cross-Cultural Adaptation and Psychometric Properties of the Chinese Version of the Knee Osteoarthritis Fears and Beliefs Questionnaire
Authors: Su Shan , Yu Clare Chung-Wah , Cheing Gladys Lai-Ying , Chung Raymond Chi-Keung , Tsang Sharon Man-Ha , Chan Lok-Lok , Tang Tracy Wing-Shan , Cheung Winky , Lee Qunn Jid , Kwong Patrick Wai-Hang
Published: January 2024

This study aimed to adapt and validate the Knee Osteoarthritis Fears and Beliefs Questionnaire (KOFBeQ) for Chinese patients, thereby advancing the understanding of fear-avoidance behaviors. Adopting a cross-sectional design, data were collected for 241 subjects (78.8% women; mean age 68.0 ± 7.8 years) across various healthcare settings in Hong Kong. Exploratory factor analysis resulted in an 11-item questionnaire with three distinct subscales, covering fears and beliefs related to physicians and disease (six items), daily living activities (three items), and sports and leisure activities (two items). The overall Cronbach’s α coefficient was 0.86, indicating strong internal consistency. The questionnaire exhibited favorable convergent validity. Confirmatory factor analyses confirmed a good model fit. Test–retest reliability analysis indicated a high intraclass correlation coefficient of 0.93 (95% confidence interval: 0.88, 0.96), and a Bland–Altman plot revealed a slight bias in two measurements (0.97 [0.19]) without a systematic trend. The adapted Chinese version of the KOFBeQ demonstrated robust psychometric properties in terms of validity and reliability, providing an effective tool for surveying Chinese patients with knee osteoarthritis. These findings offer valuable insights for clinicians and patients, aiding in informed decision-making and improved rehabilitation strategies.
Prenatal Stress as a Risk Factor for Maternal–Foetal Morbidity: A Longitudinal Study
Authors: Palomo-Gómez Rocío , Rúger-Navarrete Azahara , Antúnez-Calvente Irene , Vázquez-Lara Juana María , Rodríguez-Díaz Luciano , Gómez-Salgado Juan , Riesco-González Francisco Javier , Vázquez-Lara María Dolores , Muñoz-Vela Francisco Javier , Fernández-Carrasco Francisco Javier
Published: January 2024

Pregnancy is one of the most complex periods in a woman’s life, not only because of the biological changes involved but also because of the psychological aspects. Stress during pregnancy refers to the concerns and distress that arise during pregnancy and that can be assessed by means of psychological and physiological scales. The aim of this study was to analyse prenatal stress and to evaluate its consequences on the health of both the mother and the foetus. A descriptive longitudinal study was carried out on a sample of 398 pregnant women being followed up during their entire pregnancy, who gave birth at the Punta de Europa University Hospital in Algeciras (Spain) between September 2021 and August 2023. The Prenatal Distress Questionnaire (PDQ) was used, as well as serum cortisol levels in each trimester of pregnancy and birth experience using the Childbirth Experience Questionnaire in its validated Spanish version, CEQ-E. Demographic and obstetric variables were included. One of the main findings was that experiencing more stress in late pregnancy had a negative impact on obstetric outcomes. Women who had higher levels of prenatal distress had higher blood cortisol levels and increased risk of having a caesarean section at delivery. A significant negative correlation was also found between stress and Apgar test values in the first minute of life. It is concluded that interventions promoted by the health system that provide comprehensive prenatal care contribute to decreased stress as perceived by these pregnant women, thus reducing the risk of maternal and foetal morbidity.
How Do Care Partners of People with Rare Dementia Use Language in Online Peer Support Groups? A Quantitative Text Analysis Study
Authors: Hayes Oliver S. , El Baou Celine , Hardy Chris J. D. , Camic Paul M. , Brotherhood Emilie V. , Harding Emma , Crutch Sebastian J.
Published: January 2024

We used quantitative text analysis to examine conversations in a series of online support groups attended by care partners of people living with rare dementias (PLWRD). We used transcripts of 14 sessions (>100,000 words) to explore patterns of communication in trained facilitators’ (n = 2) and participants’ (n = 11) speech and to investigate the impact of session agenda on language use. We investigated the features of their communication via Poisson regression and a clustering algorithm. We also compared their speech with a natural speech corpus. We found that differences to natural speech emerged, notably in emotional tone (d = −3.2, p < 0.001) and cognitive processes (d = 2.8, p < 0.001). We observed further differences between facilitators and participants and between sessions based on agenda. The clustering algorithm categorised participants’ contributions into three groups: sharing experience, self-reflection, and group processes. We discuss the findings in the context of Social Comparison Theory. We argue that dedicated online spaces have a positive impact on care partners in combatting isolation and stress via affiliation with peers. We then discuss the linguistic mechanisms by which social support was experienced in the group. The present paper has implications for any services seeking insight into how peer support is designed, delivered, and experienced by participants.
Radical Left Culture and Heritage, the Politics of Preservation and Memorialisation, and the Promise of the Metaverse
Authors: Mutibwa Daniel H.
Published: January 2024

Radical left culture and heritage—understood as incarnations of leftist artefacts and praxis both past and present—have taken risks in challenging hegemonic machinations often when it is unpopular to do so. To the ire of hegemons, leftist projects across the globe have marshalled places, spaces, and technologies into sites of empowerment and struggle utilising ‘small’ and ‘big’ acts of resistance and critical interventions to champion social justice—sometimes successfully, and at other times, less so. However, the preservation of projects’ artefacts, praxis, and memory work has been anything but straightforward, owing primarily to institutional politics and infrastructural and resourcing issues. Taking The Freedom Archives (FA) as a case study, this article explores how FA is preserving the distinctive political education programme that underpinned the iconic liberation struggle in Guinea-Bissau that kickstarted the seismic, global decolonisation project in the late 1950s. The article argues that FA could substantially enhance the preservation and memorialisation of that programme in the Metaverse—if this materialises as a fully open, interoperable, and highly immersive space (1) unfettered by hegemonic regulation, and (2) characterised by ‘strategic witnessing’, ‘radical recordkeeping’, and user agency. In doing so, FA would serve as an exemplar for leftist projects globally.
Detection of Strobilurin Fungicides in Trout Streams within an Agricultural Watershed
Authors: Weaver Cole R. , Brockman Meghan , Mundahl Neal D. , Arnold William A. , Blumentritt Dylan , Varela Will L. , Franz Jeanne L.
Published: January 2024

The use of strobilurin fungicides in agriculture has increased steadily during the past 25 years, and although strobilurins have minimal water solubility, they regularly appear in surface waters, at times in concentrations approaching toxic levels for aquatic life. The present study examined concentrations of strobilurin fungicides in designated trout streams draining an agricultural watershed in southeastern Minnesota, USA, where fungicides may have contributed to a recent fish kill. Water samples (n = 131) were analyzed for the presence of five different strobilurin fungicides (azoxystrobin, fluoxastrobin, picoxystrobin, pyraclostrobin, trifloxystrobin). Samples were collected via grab and automated sampling during baseflow and stormflow events throughout an entire crop-growing season from sites on each of the three forks of the Whitewater River. Detection frequencies for the five strobilurins ranged from 44 to 82%. Fluoxastrobin and pyraclostrobin concentrations were above known toxic levels in 3% and 15% of total samples analyzed, respectively. The highest concentrations were detected in mid-summer (mid-June to mid-August) samples, coincident with likely strobilurin applications. Lower concentrations were present in water samples collected during the nonapplication periods in spring and fall, suggesting groundwater–stream interactions or steady leaching of fungicides from watershed soils or stream sediments. Further study is required to determine strobilurin concentrations in sediments, soils, and groundwater. Better tracking and guidance regarding strobilurin use is necessary to adequately protect aquatic life as fungicide use continues to increase.
Implementation and Validation of an OpenMM Plugin for the Deep Potential Representation of Potential Energy
Authors: Ding Ye , Huang Jing
Published: January 2024

Machine learning potentials, particularly the deep potential (DP) model, have revolutionized molecular dynamics (MD) simulations, striking a balance between accuracy and computational efficiency. To facilitate the DP model’s integration with the popular MD engine OpenMM, we have developed a versatile OpenMM plugin. This plugin supports a range of applications, from conventional MD simulations to alchemical free energy calculations and hybrid DP/MM simulations. Our extensive validation tests encompassed energy conservation in microcanonical ensemble simulations, fidelity in canonical ensemble generation, and the evaluation of the structural, transport, and thermodynamic properties of bulk water. The introduction of this plugin is expected to significantly expand the application scope of DP models within the MD simulation community, representing a major advancement in the field.
Genome Identification and Expression Profiling of the PIN-Formed Gene Family in Phoebe bournei under Abiotic Stresses
Authors: Li Jingshu , Zhang Yanzi , Tang Xinghao , Liao Wenhai , Li Zhuoqun , Zheng Qiumian , Wang Yanhui , Chen Shipin , Zheng Ping , Cao Shijiang
Published: January 2024

PIN-formed (PIN) proteins—specific transcription factors that are widely distributed in plants—play a pivotal role in regulating polar auxin transport, thus influencing plant growth, development, and abiotic stress responses. Although the identification and functional validation of PIN genes have been extensively explored in various plant species, their understanding in woody plants—particularly the endangered species Phoebe bournei (Hemsl.) Yang—remains limited. P. bournei is an economically significant tree species that is endemic to southern China. For this study, we employed bioinformatics approaches to screen and identify 13 members of the PIN gene family in P. bournei. Through a phylogenetic analysis, we classified these genes into five sub-families: A, B, C, D, and E. Furthermore, we conducted a comprehensive analysis of the physicochemical properties, three-dimensional structures, conserved motifs, and gene structures of the PbPIN proteins. Our results demonstrate that all PbPIN genes consist of exons and introns, albeit with variations in their number and length, highlighting the conservation and evolutionary changes in PbPIN genes. The results of our collinearity analysis indicate that the expansion of the PbPIN gene family primarily occurred through segmental duplication. Additionally, by predicting cis-acting elements in their promoters, we inferred the potential involvement of PbPIN genes in plant hormone and abiotic stress responses. To investigate their expression patterns, we conducted a comprehensive expression profiling of PbPIN genes in different tissues. Notably, we observed differential expression levels of PbPINs across the various tissues. Moreover, we examined the expression profiles of five representative PbPIN genes under abiotic stress conditions, including heat, cold, salt, and drought stress. These experiments preliminarily verified their responsiveness and functional roles in mediating responses to abiotic stress. In summary, this study systematically analyzes the expression patterns of PIN genes and their response to abiotic stresses in P. bournei using whole-genome data. Our findings provide novel insights and valuable information for stress tolerance regulation in P. bournei. Moreover, the study offers significant contributions towards unraveling the functional characteristics of the PIN gene family.
Long-Term Cryopreservation May Cause Genomic Instability and the Premature Senescence of Cells
Authors: Shorokhova Mariia , Pugovkina Natalia , Zemelko Victoria , Lyublinskaya Olga , Grinchuk Tatiana
Published: January 2024

Cryopreservation is an essential step for utilizing various cell types for biological research and medical purposes. At the same time, there is a lack of data on the effect of cryopreservation, especially when prolonged, on the karyotype of cells. In the present work, we analyzed the genetic stability of cells subjected to a cryopreservation procedure. The objects were immortalized Chinese hamster lung fibroblasts (CHL V-79 RJK line) and human endometrial mesenchymal stem/stromal cells (eMSCs). We showed that short-term cryopreservation in liquid nitrogen for up to 6 months did not affect the karyotype stability of CHL V-79 RJK and eMSCs. On the contrary, karyotyping of G-banded metaphase chromosomes in cells underwent 10-year cryopreservation, which revealed genomic instability in both cell lines associated with the variability of chromosome number in cells, random chromosomal rearrangements, and condensation disorder in homologs. In addition, we found out that long-term cryopreservation of eMSCs does not affect the expression of their typical surface markers and morphology, but results in a significant reduction in proliferative potential and early manifestation of cellular senescence features upon eMSCs culturing. Thus, we concluded that the long-term cryopreservation of cells of different types and biological origin can lead to irreversible changes of their karyotype and acceleration of cellular senescence.
Trials and Tribulations of MicroRNA Therapeutics
Authors: Seyhan Attila A.
Published: January 2024

The discovery of the link between microRNAs (miRNAs) and a myriad of human diseases, particularly various cancer types, has generated significant interest in exploring their potential as a novel class of drugs. This has led to substantial investments in interdisciplinary research fields such as biology, chemistry, and medical science for the development of miRNA-based therapies. Furthermore, the recent global success of SARS-CoV-2 mRNA vaccines against the COVID-19 pandemic has further revitalized interest in RNA-based immunotherapies, including miRNA-based approaches to cancer treatment. Consequently, RNA therapeutics have emerged as highly adaptable and modular options for cancer therapy. Moreover, advancements in RNA chemistry and delivery methods have been pivotal in shaping the landscape of RNA-based immunotherapy, including miRNA-based approaches. Consequently, the biotechnology and pharmaceutical industry has witnessed a resurgence of interest in incorporating RNA-based immunotherapies and miRNA therapeutics into their development programs. Despite substantial progress in preclinical research, the field of miRNA-based therapeutics remains in its early stages, with only a few progressing to clinical development, none reaching phase III clinical trials or being approved by the US Food and Drug Administration (FDA), and several facing termination due to toxicity issues. These setbacks highlight existing challenges that must be addressed for the broad clinical application of miRNA-based therapeutics. Key challenges include establishing miRNA sensitivity, specificity, and selectivity towards their intended targets, mitigating immunogenic reactions and off-target effects, developing enhanced methods for targeted delivery, and determining optimal dosing for therapeutic efficacy while minimizing side effects. Additionally, the limited understanding of the precise functions of miRNAs limits their clinical utilization. Moreover, for miRNAs to be viable for cancer treatment, they must be technically and economically feasible for the widespread adoption of RNA therapies. As a result, a thorough risk evaluation of miRNA therapeutics is crucial to minimize off-target effects, prevent overdosing, and address various other issues. Nevertheless, the therapeutic potential of miRNAs for various diseases is evident, and future investigations are essential to determine their applicability in clinical settings.
Long Noncoding RNA VLDLR-AS1 Levels in Serum Correlate with Combat-Related Chronic Mild Traumatic Brain Injury and Depression Symptoms in US Veterans
Authors: Patel Rekha S. , Krause-Hauch Meredith , Kenney Kimbra , Miles Shannon , Nakase-Richardson Risa , Patel Niketa A.
Published: January 2024

More than 75% of traumatic brain injuries (TBIs) are mild (mTBI) and military service members often experience repeated combat-related mTBI. The chronic comorbidities concomitant with repetitive mTBI (rmTBI) include depression, post-traumatic stress disorder or neurological dysfunction. This study sought to determine a long noncoding RNA (lncRNA) expression signature in serum samples that correlated with rmTBI years after the incidences. Serum samples were obtained from Long-Term Impact of Military-Relevant Brain-Injury Consortium Chronic Effects of Neurotrauma Consortium (LIMBIC CENC) repository, from participants unexposed to TBI or who had rmTBI. Four lncRNAs were identified as consistently present in all samples, as detected via droplet digital PCR and packaged in exosomes enriched for CNS origin. The results, using qPCR, demonstrated that the lncRNA VLDLR-AS1 levels were significantly lower among individuals with rmTBI compared to those with no lifetime TBI. ROC analysis determined an AUC of 0.74 (95% CI: 0.6124 to 0.8741; p = 0.0012). The optimal cutoff for VLDLR-AS1 was ≤153.8 ng. A secondary analysis of clinical data from LIMBIC CENC was conducted to evaluate the psychological symptom burden, and the results show that lncRNAs VLDLR-AS1 and MALAT1 are correlated with symptoms of depression. In conclusion, lncRNA VLDLR-AS1 may serve as a blood biomarker for identifying chronic rmTBI and depression in patients.
Fluoride-Incorporated Apatite Coating on Collagen Sponge as a Carrier for Basic Fibroblast Growth Factor
Authors: Pal Aniruddha , Oyane Ayako , Nakamura Maki , Koga Kenji , Nishida Erika , Miyaji Hirofumi
Published: January 2024

Coating layers consisting of a crystalline apatite matrix with immobilized basic fibroblast growth factor (bFGF) can release bFGF, thereby enhancing bone regeneration depending on their bFGF content. We hypothesized that the incorporation of fluoride ions into apatite crystals would enable the tailored release of bFGF from the coating layer depending on the layer’s fluoride content. In the present study, coating layers consisting of fluoride-incorporated apatite (FAp) crystals with immobilized bFGF were coated on a porous collagen sponge by a precursor-assisted biomimetic process using supersaturated calcium phosphate solutions with various fluoride concentrations. The fluoride content in the coating layer increased with the increasing fluoride concentration of the supersaturated solution. The increased fluoride content in the coating layer reduced its solubility and suppressed the burst release of bFGF from the coated sponge into a physiological salt solution. The bFGF release was caused by the partial dissolution of the coating layer and, thus, accompanied by the fluoride release. The concentrations of released bFGF and fluoride were controlled within the estimated effective ranges in enhancing bone regeneration. These findings provide useful design guidelines for the construction of a mineralized, bFGF-releasing collagen scaffold that would be beneficial for bone tissue engineering, although further in vitro and in vivo studies are warranted.
Identification of a Panel of miRNAs Associated with Resistance to Palbociclib and Endocrine Therapy
Authors: Torrisi Rosalba , Vaira Valentina , Giordano Laura , Fernandes Bethania , Saltalamacchia Giuseppe , Palumbo Raffaella , Carnaghi Carlo , Basilico Vera , Gentile Francesco , Masci Giovanna , De Sanctis Rita , Santoro Armando
Published: January 2024

We investigated whether we could identify a panel of miRNAs associated with response to treatment in tumor tissues of patients with Hormone Receptor-positive/HER2-negative metastatic breast cancer treated with endocrine therapy (ET) and the CDK4/6 inhibitor (CDK4/6i)i palbociclib. In total, 52 patients were evaluated, with 41 receiving treatment as the first line. The overall median PFS was 20.8 months (range 2.5–66.6). In total, 23% of patients experienced early progression (<6 months). Seven miRNAs (miR-378e, miR-1233, miR-99b-5p, miR-1260b, miR-448, -miR-1252-5p, miR-324-3p, miR-1233-3p) showed a statistically significant negative association with PFS. When we considered PFS < 6 months, miR-378e, miR-99b-5p, miR-877-5p, miR-1297, miR-455-5p, and miR-4536-5p were statistically associated with a poor outcome. In the multivariate analysis, the first three miRNAs confirmed a significant and independent impact on PFS. The literature data and bioinformatic tools provide an underlying molecular rationale for most of these miRNAs, mainly involving the PI3K/AKT/mTOR pathway and cell-cycle machinery as cyclin D1, CDKN1B, and protein p27Kip1 and autophagy. Our findings propose a novel panel of miRNAs associated with a higher likelihood of early progression in patients treated with ET and Palbociclib and may contribute to shed some light on the mechanisms of de novo resistance to CDK4/6i, but this should be considered exploratory and evaluated in larger cohorts.
The Expression of Alamandine Receptor MrgD in Clear Cell Renal Cell Carcinoma Is Associated with a Worse Prognosis and Unfavorable Response to Antiangiogenic Therapy
Authors: Larrinaga Gorka , Valdivia Asier , Arrieta-Aguirre Inés , Solano-Iturri Jon Danel , Ugalde-Olano Aitziber , Loizaga-Iriarte Ana , Santos-Martín Aida , Pérez-Fernández Amparo , Angulo Javier C. , López José I.
Published: January 2024

Renal cell carcinoma (RCC) ranks among the most prevalent malignancies in Western countries, marked by its notable heterogeneity, which contributes to an unpredictable clinical trajectory. The insufficiency of dependable biomarkers adds complexity to assessing this tumor progression. Imbalances of several components of the intrarenal renin–angiotensin system (iRAS) significantly impact patient prognoses and responses to first-line immunotherapies. In this study, we analyzed the immunohistochemical expression of the Mas-related G-protein-coupled receptor D (MrgD), which recognizes the novel RAS peptide alamandine (ALA), in a series of 87 clear cell renal cell (CCRCCs), 19 papillary (PRCC), 7 chromophobe (ChRCC) renal cell carcinomas, and 11 renal oncocytomas (RO). MrgD was expressed in all the renal tumor subtypes, with a higher mean staining intensity in the PRCCs, ChRCCs, and ROs. A high expression of MrgD at the tumor center and at the infiltrative front of CCRCC tissues was significantly associated with a high histological grade, large tumor diameter, local invasion, and locoregional node and distant metastasis. Patients with worse 5-year cancer-specific survival and a poorer response to antiangiogenic tyrosine-kinase inhibitors (TKIs) showed higher MrgD expression at the center of their primary tumors. These findings suggest a possible role of MrgD in renal carcinogenetic processes. Further studies are necessary to unveil its potential as a novel biomarker for CCRCC prognosis and response to frontline therapies.
Durability, Capillary Rise and Water Absorption Properties of a Fiber-Reinforced Cement-Stabilized Fly Ash–Stone Dust Mixture
Authors: Mohanty Sanjeeb Kumar , Pandit Nirmal Kumar , Sah Pawan Kumar , Mahaseth Niraj , Yadav Rajesh , Biswal Dipti Ranjan , Mohapatra Benu Gopal , Beriha Brundaban , Pradhan Ramachandra , Pradhan Sujit Kumar
Published: January 2024

The management of unutilized fly ash poses challenges due to concerns about storage and its potential groundwater contamination. Within the road industry, where the bulk utilization of fly ash is feasible, its unsuitability for use in the base and sub-base layers of pavements due to its low strength and a high proportion of fine particles has been a limitation. The incorporation of stone dust alongside fly ash, treated with lime or cement, yields superior strength and stiffness. Apart from strength, the stabilized mix’s durability, capillary rise, and water absorption properties are crucial for determining its suitability for pavement applications. Observations from this study reveal that fiber-reinforced cement-stabilized fly ash–stone aggregate specimens treated with 4% and 6% cement, with and without fibers, met the limiting mass loss of 20%, as specified in IRC SP: 89. The mass loss decreases with an increase in cement and fiber content. However, the capillary rise in the mixes increases with a higher percentage of fly ash and fiber content but decreases with increased cement content. Cement addition results in a reduction in water absorption; however, the addition of fibers results in an increase in water absorption. A linear correlation has been established between mass loss and UCS and IDT, which can be used to evaluate the suitability of materials for the structural layer without conducting a wet–dry durability test, which typically takes one month. This study proposes that cement-stabilized fly ash and stone aggregate mixtures with 4% and 6% cement can be used as the subbase and base of pavement based on wet–dry mass loss criteria and water absorption criteria. Observations from this study reveal that fiber-reinforced cement-stabilized fly ash–stone aggregate specimens treated with 4% and 6% cement, with and without fibers, met the limiting mass loss of 20%, as specified in IRC SP: 89. The mass loss decreases with an increase in cement and fiber content. However, the capillary rise in the mixes increases with a higher percentage of fly ash and fiber content but decreases with increased cement content. Cement addition results in reduction in water absorption. However, the addition of fibers results in increase in water absorption. A linear correlation is established between mass loss and UCS and IDT, which can be used to evaluate the suitability of materials for the structural layer without conducting wet–dry durability tests, which take one month. This study proposes that cement-stabilized fly ash and stone aggregate mixtures with 4% and 6% cement can be used as the subbase and base of pavement based on wet–dry mass loss criteria and water absorption criteria.