bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Insights into Biofilm Formation by a Pathogenic Strain of Vibrio harveyi
Authors: Morot Amandine , Delavat François , Bazire Alexis , Paillard Christine , Dufour Alain , Rodrigues Sophie
Published: January 2024

The Vibrio genus includes bacteria widely distributed in aquatic habitats and the infections caused by these bacteria can affect a wide range of hosts. They are able to adhere to numerous surfaces, which can result in biofilm formation that helps maintain them in the environment. The involvement of the biofilm lifestyle in the virulence of Vibrio pathogens of aquatic organisms remains to be investigated. Vibrio harveyi ORM4 is a pathogen responsible for an outbreak in European abalone Haliotis tuberculata populations. In the present study, we used a dynamic biofilm culture technique coupled with laser scanning microscopy to characterize the biofilm formed by V. harveyi ORM4. We furthermore used RNA-seq analysis to examine the global changes in gene expression in biofilm cells compared to planktonic bacteria, and to identify biofilm- and virulence-related genes showing altered expression. A total of 1565 genes were differentially expressed, including genes associated with motility, polysaccharide synthesis, and quorum sensing. The up-regulation of 18 genes associated with the synthesis of the type III secretion system suggests that this virulence factor is induced in V. harveyi ORM4 biofilms, providing indirect evidence of a relationship between biofilm and virulence.
Molecular Surveillance for Bocaparvoviruses and Bufaviruses in the European Hedgehog (Erinaceus europaeus)
Authors: Sarchese Vittorio , Palombieri Andrea , Prandi Ilaria , Robetto Serena , Bertolotti Luigi , Capucchio Maria Teresa , Orusa Riccardo , Mauthe von Degerfeld Mitzy , Quaranta Giuseppe , Vacchetta Massimo , Martella Vito , Di Martino Barbara , Di Profio Federica
Published: January 2024

The presence of bocaparvoviruses (BoVs) and bufaviruses (BuVs) in the European hedgehog (Erinaceus europaeus) was investigated by screening duodenal and liver samples collected from 183 carcasses, delivered to wildlife rescue centers located in northwestern Italy. BoV DNA was detected in 15 animals (8.2%), with prevalences of 7.1% (13/183) and 2.7% (5/183) in intestine and liver samples, respectively. Upon the sequence analyses of the NS1 gene, two highly divergent BoVs (65.5–67.8% nt identities) were identified. Fourteen strains showed the highest identity (98.3–99.4% nt) to the hedgehog BoV strains recently detected in China in Amur hedgehogs (Erinaceus amurensis), whilst four strains were genetically related (98.9–99.4% nt identities) to the porcine BoVs identified in pigs and classified in the species Bocaparvovirus ungulate 4, which included related viruses also found in rats, minks, shrews, and mice. BuV DNA was detected in the duodenal samples of two hedgehogs, with a prevalence rate of 1.1%. The nearly full-length genome of two BuV strains, Hedgehog/331DU-2022/ITA and Hedgehog/1278DU/2019/ITA, was reconstructed. Upon phylogenetic analysis based on the NS and VP aa sequences, the Italian hedgehog BuVs tightly clustered with the BuVs recently identified in the Chinese Amur hedgehogs, within a potential novel candidate species of the genus Protoparvovirus.
Estimation of Geotechnical Parameters for Coal Exploration from Quasi-3D Electrical Resistivity Measurements
Authors: Rupesh Rupesh , Tiwari Prarabdh , Sharma Shashi Prakash
Published: January 2024

Geotechnical parameters are crucial for mine planning and operation at different stages of development. However, estimating these parameters requires a large number of boreholes and subsequent detailed analysis of the samples, making it a cumbersome exercise. Moreover, even after conducting these studies, it is not possible to cover the entire operational area. To address this issue, this study presents an indirect method of estimating geotechnical parameters through mathematical relations using resistivity data. The present study incorporated 2D and 3D subsurface imaging techniques for exploring coal reserves and analyzing geotechnical parameters that define subsurface soil properties. Electrical resistivity tomography (ERT) was utilized for data acquisition, employing a Dipole–dipole array with a multielectrode ABEM Terrameter LS instrument. Six parallel profiles were conducted, each 400 m in length, with an inter-electrode spacing of 10 m and a spacing of 50 m between profiles. These profiles were combined into a 3D dataset referred to as quasi-3D ERT. The inversion process for both 2D and 3D data was performed using the Res2dinv and Res3dinv programs, respectively. This study overcame the challenges of 2D resistivity sections by evaluating horizontal depth slices in the x-z plane from layers 1 to 10, reaching a depth of 81.2 m. The geotechnical parameters, including cohesion, friction angle, moisture content, and plastic index, were derived from the resistivity data. The ERT method proved to be cost-effective and efficient in determining soil properties over a large area compared with traditional laboratory analysis of borehole samples. Additionally, the variation of geotechnical parameters with resistivity values exhibited unique characteristics. The results from both the 2D and quasi-3D ERT were well correlated with the borehole data. Such studies are valuable for resource exploration and mine planning purposes.
Aluminium 8-Hydroxyquinolinate N-Oxide as a Precursor to Heterometallic Aluminium–Lanthanide Complexes
Authors: Gallo Elisa , Bellucci Luca , Carlotto Silvia , Bottaro Gregorio , Babetto Luca , Giordano Luca , Marchetti Fabio , Samaritani Simona , Armelao Lidia , Labella Luca
Published: January 2024

A reaction in anhydrous toluene between the formally unsaturated fragment [Ln(hfac)3] (Ln3+ = Eu3+, Gd3+ and Er3+; Hhfac = hexafluoroacetylacetone) and [Al(qNO)3] (HqNO = 8-hydroxyquinoline N-oxide), here prepared for the first time from [Al(OtBu)3] and HqNO, affords the dinuclear heterometallic compounds [Ln(hfac)3Al(qNO)3] (Ln3+ = Eu3+, Gd3+ and Er3+) in high yields. The molecular structures of these new compounds revealed a dinuclear species with three phenolic oxygen atoms bridging the two metal atoms. While the europium and gadolinium complexes show the coordination number (CN) 9 for the lanthanide centre, in the complex featuring the smaller erbium ion, only two oxygens bridge the two metal atoms for a resulting CN of 8. The reaction of [Eu(hfac)3] with [Alq3] (Hq = 8-hydroxyquinoline) in the same conditions yields a heterometallic product of composition [Eu(hfac)3Alq3]. A recrystallization attempt from hot heptane in air produced single crystals of two different morphologies and compositions: [Eu2(hfac)6Al2q4(OH)2] and [Eu2(hfac)6(µ-Hq)2]. The latter compound can be directly prepared from [Eu(hfac)3] and Hq at room temperature. Quantum mechanical calculations confirm (i) the higher stability of [Eu(hfac)3Al(qNO)3] vs. the corresponding [Eu(hfac)3Alq3] and (ii) the preference of the Er complexes for the CN 8, justifying the different behaviour in terms of the Lewis acidity of the metal centre.
Immunocytochemical Analysis of Crocin against Oxidative Stress in Trigeminal Sensory Neurons Innervating the Cornea
Authors: Sánchez-Fernández Cristina , Del Olmo-Aguado Susana , Artime Enol , Barros Alberto , Fernández-Vega Cueto Luis , Merayo-Lloves Jesús , Alcalde Ignacio
Published: January 2024

Corneal diseases are a major cause of vision loss, often associated with aging, trauma and disease. Damage to corneal sensory innervation leads to discomfort and pain. Environmental stressors, such as short-wavelength light, can induce oxidative stress that alters mitochondrial function and affects cell and tissue homeostasis, including corneal innervation. Cellular antioxidant mechanisms may attenuate oxidative stress. This study investigates crocin, a derivative of saffron, as a potential antioxidant therapy. In vitro rat trigeminal sensory ganglion neurons were exposed to both sodium azide and blue light overexposure as a model of oxidative damage. Crocin was used as a neuroprotective agent. Mitochondrial and cytoskeletal markers were studied by immunofluorescence analysis to determine oxidative damage and neuroprotection. In vivo corneal innervation degeneration was evaluated in cornea whole mount preparations using Sholl analyses. Blue light exposure induces oxidative stress that affects trigeminal neuron mitochondria and alters sensory axon dynamics in vitro, and it also affects corneal sensory innervation in an in vivo model. Our results show that crocin was effective in preserving mitochondrial function and protecting corneal sensory neurons from oxidative stress. Crocin appears to be a promising candidate for the neuroprotection of corneal innervation.
Al/SBA-15 Mesoporous Material: A Study of pH Influence over Aluminum Insertion into the Framework
Authors: Pinto Francisco Gustavo Hayala Silveira , Caldeira Vinícius Patrício da Silva , Villarroel-Rocha Jhonny , Sapag Karim , Pergher Sibele Berenice Castellã , Santos Anne Gabriella Dias
Published: January 2024

Herein, ordered mesoporous materials like SBA-15 and Al/SBA-15 were prepared using the pH adjustment method. Thus, these materials were developed in different pH of synthesis, from the pH adjustment method using a KCl/HCl solution and varying the Si/Al molar ratio (5, 25, and 75). All the ordered mesoporous materials were characterized by FRX, 27Al NMR, SEM, XRD, N2 adsorption/desorption, and CO2 adsorption. From the applied method, it was possible to obtain SBA-15 and Al/SBA-15 with high mesoscopic ordering based on the XRD patterns, independent of the pH employed. From the chemical composition, the insertion of higher amounts of Al into the synthesis caused a progressive improvement in the structural and textural properties of the ordered mesoporous materials. Thus, the chosen synthesis conditions can lead to different aluminum coordination (tetrahedral and octahedral), which gives these materials a greater potential to be applied. The presence of Al in high amounts provides material with the ability to form micropores. Finally, the proposed method proved to be innovative; low-cost; less aggressive to the environment, with efficient insertion of aluminum in the framework of SBA-15 mesoporous material; and practical, based on only one step.
Recent Progress of Bioinspired Triboelectric Nanogenerators for Electronic Skins and Human–Machine Interaction
Authors: Zhang Baosen , Jiang Yunchong , Chen Baojin , Li Haidong , Mao Yanchao
Published: January 2024

Advances in biomimetic triboelectric nanogenerators (TENGs) have significant implications for electronic skin (e-skin) and human–machine interaction (HMI). Emphasizing the need to mimic complex functionalities of natural systems, particularly human skin, TENGs leverage triboelectricity and electrostatic induction to bridge the gap in traditional electronic devices’ responsiveness and adaptability. The exploration begins with an overview of TENGs’ operational principles and modes, transitioning into structural and material biomimicry inspired by plant and animal models, proteins, fibers, and hydrogels. Key applications in tactile sensing, motion sensing, and intelligent control within e-skins and HMI systems are highlighted, showcasing TENGs’ potential in revolutionizing wearable technologies and robotic systems. This review also addresses the challenges in performance enhancement, scalability, and system integration of TENGs. It points to future research directions, including optimizing energy conversion efficiency, discovering new materials, and employing micro-nanostructuring techniques for enhanced triboelectric charges and energy conversion. The scalability and cost-effectiveness of TENG production, pivotal for mainstream application, are discussed along with the need for versatile integration with various electronic systems. The review underlines the significance of making bioinspired TENGs more accessible and applicable in everyday technology, focusing on compatibility, user comfort, and durability. Conclusively, it underscores the role of bioinspired TENGs in advancing wearable technology and interactive systems, indicating a bright future for these innovations in practical applications.
From Birth to Weaning: A Window of Opportunity for Microbiota
Authors: Biagioli Valentina , Volpedo Greta , Riva Antonella , Mainardi Paolo , Striano Pasquale
Published: January 2024

(1) Background: The first 1000 days of life constitute a critical window of opportunity for microbiota development. Nutrients play a crucial role in enriching and diversifying the microbiota, derived not only from solid food but also from maternal dietary patterns during gestation. (2) Methods: We conducted a comprehensive literature review using the PubMed database, covering eleven years (2013–2023). We included English-language reviews, original research papers, and meta-analyses, while excluding case reports and letters. (3) Results: Consensus in the literature emphasizes that our interaction with a multitude of microorganisms begins in the intrauterine environment and continues throughout our lives. The existing data suggest that early nutritional education programs, initiated during pregnancy and guiding infant diets during development, may influence the shaping of the gut microbiota, promoting long-term health. (4) Conclusions: Further research is necessary in the coming years to assess potential interventions and early nutritional models aimed at modulating the pediatric microbiota, especially in vulnerable populations such as premature newborns.
Synergistic Effect of a Flavonoid-Rich Cocoa–Carob Blend and Metformin in Preserving Pancreatic Beta Cells in Zucker Diabetic Fatty Rats
Authors: Gallardo-Villanueva Paula , Fernández-Marcelo Tamara , Villamayor Laura , Valverde Angela M. , Ramos Sonia , Fernández-Millán Elisa , Martín María Angeles
Published: January 2024

The loss of functional beta-cell mass in diabetes is directly linked to the development of diabetic complications. Although dietary flavonoids have demonstrated antidiabetic properties, their potential effects on pancreatic beta-cell preservation and their synergistic benefits with antidiabetic drugs remain underexplored. We have developed a potential functional food enriched in flavonoids by combining cocoa powder and carob flour (CCB), which has shown antidiabetic effects. Here, we investigated the ability of the CCB, alone or in combination with metformin, to preserve pancreatic beta cells in an established diabetic context and their potential synergistic effect. Zucker diabetic fatty rats (ZDF) were fed a CCB-rich diet or a control diet, with or without metformin, for 12 weeks. Markers of pancreatic oxidative stress and inflammation, as well as relative beta-cell mass and beta-cell apoptosis, were analyzed. Results demonstrated that CCB feeding counteracted pancreatic oxidative stress by enhancing the antioxidant defense and reducing reactive oxygen species. Moreover, the CCB suppressed islet inflammation by preventing macrophage infiltration into islets and overproduction of pro-inflammatory cytokines, along with the inactivation of nuclear factor kappa B (NFκB). As a result, the CCB supplementation prevented beta-cell apoptosis and the loss of beta cells in ZDF diabetic animals. The observed additive effect when combining the CCB with metformin underscores its potential as an adjuvant therapy to delay the progression of type 2 diabetes.
Competitive Foods’ Nutritional Quality and Compliance with Smart Snacks Standards: An Analysis of a National Sample of U.S. Middle and High Schools
Authors: Cohen Juliana F. W. , Kesack Ashley , Daly Tara P. , Elnakib Sara A. , Hager Erin , Hahn Samuel , Hamlin Daniel , Hill Alla , Lehmann Annie , Lurie Peter , Maroney Meghan , Means Jaydn , Mueller Megan P. , Olarte Deborah A. , Polacsek Michele , Schwartz Marlene B. , Sonneville Kendrin R. , Spruance Lori A. , Woodward Andrea R. , Chapman Leah E.
Published: January 2024

Snacks and beverages are often sold in addition to meals in U.S. schools (“competitive foods”), but their current nutritional quality and compliance with national Smart Snacks standards are unknown. This study assessed competitive foods in a national sample of 90 middle and high schools. Differences in compliance by school characteristics were measured using mixed methods analysis of variance. Overall, 80% of the schools in the sample sold competitive foods; but they were less commonly available in schools with universal free school meal (UFSM) policies. A total of 840 unique products were documented and, on average, 75% were compliant with Smart Snacks standards. A total of 56% aligned with recommended added sugar limits (<10% of calories); and 340 unique products (40%) aligned with both sugar and Smart Snacks standards. Approximately one-fifth of competitive foods contained synthetic dyes, and 31% of beverages contained artificial sweeteners. Smart Snacks standards compliance was greater when competitive foods were overseen by food service departments, in comparison with others (e.g., principals, student organizations, or outside vendors [77% vs. 59% compliance; p = 0.003]). Therefore, district wellness policies should consider requiring food service departments to oversee competitive foods. Federal and state policies should limit added sugars, artificial sweeteners, and synthetic dyes. This appears to be highly feasible, given the substantial number of products that meet these criteria. UFSM policies should also be considered to support healthier school meal environments more broadly.
Altered Nutrient Composition of Lactose-Reduced Infant Formula
Authors: Mokhtari Pari , Schmidt Kelsey A. , Babaei Mahsa , Goran Michael I.
Published: January 2024

This research comprehensively examines 88 infant formulas available in the US market, with an emphasis on their diverging nutritional attributes based on lactose content. We stratified formulas into three categories: lactose-free, lactose-reduced, and entirely lactose-based. The formulas’ nutritional content for 58 nutrients was obtained from the Nutrition Data System for Research (NDSR). Nutritional analysis revealed significant differences in nutrient composition across formula categories. For example, the results showed significant associations between the lactose content and glycemic index (GI) of the formula as well as 25 other nutrients. Specifically, we showed that for every gram of lactose per 100 g of formula that is removed, there was a 10.1% increase in GI (β = −10.12, p ≤ 0.000), a 19%,5%, and a 2% increase in added sugar (β = −0.19, p < 0.01), protein (β = −0.05, p < 0.001), and polyunsaturated fatty acids (β = −0.01, p < 0.01). The substitution of lactose in infant formulas significantly alters their nutritional profile, inducing changes in GI, added sugar, protein, and polyunsaturated fatty acids. These modifications have potential consequences for infant growth and metabolic responses and could influence long-term health trajectories. The clinical relevance of the composition differences between formulas should be further explored.
Impact of Medical School on the Relationship between Nutritional Knowledge and Sleep Quality—A Longitudinal Study of Students at Wroclaw Medical University in Poland
Authors: Kosendiak Aureliusz Andrzej , Adamczak Bartosz Bogusz , Kuźnik Zofia , Makles Szymon
Published: January 2024

The aim of this study was to investigate the impact of the first year of medical school on the relationship between nutritional knowledge and sleep. To achieve this, first-year medical students at Wroclaw Medical University were invited to participate in the study during both the initial and final months of their first academic year. The study included 570 students in the initial period and 705 in the latter. The research questionnaire comprised the KomPAN, assessing nutritional knowledge, and the Pittsburgh Sleep Quality Index (PSQI), evaluating sleep quality. The majority of students demonstrated at least sufficient nutritional knowledge, while approximately two-thirds of students experienced poor sleep in both periods. Notably, sleep quality further deteriorated in the second period (PSQI total score: 6.86 vs. 7.38, p = 0.0157). This change was influenced mainly by a decrease in sleep duration and an increase in the use of sleep medications (both p < 0.0001). The significant difference in overall sleep quality between different nutritional knowledge levels emerged only in the second semester, where students with the highest nutritional knowledge slept the best, while those with the lowest slept the worst (p = 0.0001). Crucially, in both periods, the use of sleep medications was highest among individuals with insufficient nutritional knowledge. Throughout the academic year, the usage increased for all except those with the highest nutritional knowledge, who exhibited the best sleep (p < 0.0001). The escalating use of sleep medications among medical students warrants greater attention, and leveraging the relationship between nutritional knowledge and sleep could prove beneficial, as positive habits in one domain may positively influence the other.
Stool Gluten Peptide Detection Is Superior to Urinary Analysis, Coeliac Serology, Dietary Adherence Scores and Symptoms in the Detection of Intermittent Gluten Exposure in Coeliac Disease: A Randomised, Placebo-Controlled, Low-Dose Gluten Challenge Study
Authors: Russell Amy K. , Lucas Erin C. , Henneken Lee M. , Pizzey Catherine J. , Clarke Dean , Myleus Anna , Tye-Din Jason A.
Published: January 2024

Monitoring adherence to a gluten-free diet is an important goal of coeliac disease management. Urine and stool gluten immunogenic peptide (GIP) assays provide an objective readout of gluten ingestion, with the former favoured due to its convenience and acceptability. This study assessed stool GIP excretion after low-dose gluten challenge designed to mimic accidental gluten exposure. A total of 52 coeliac participants undertook a randomised, double-blind gluten (50–1000 mg) or placebo challenge. Stool and urinary GIP, serology, dietary adherence and symptoms were assessed. Stool GIP was 100% sensitive for gluten intake ≥250 mg and 71% for 50 mg. Peak GIP detection was 12–36 h after gluten exposure. The mean stool GIP after 1000 mg gluten ingestion remained above the limit of quantification for 5 days. Urine GIP assessment had poor sensitivity for GIP excretion compared to stool. Serology, dietary adherence score and symptoms did not correlate with gluten excretion during lead-in. We conclude that stool GIP detection is highly sensitive, with levels related to gluten dose and time from ingestion. Weekly or bi-weekly testing will detect low-level exposure more effectively than urine GIP assessments or traditional methods. In this seronegative, apparently well-treated cohort, a high frequency of baseline-positive GIP suggests ongoing gluten exposure, but the assessment of patient behaviour and assay specificity is needed.
Coinfection of Babesia and Borrelia in the Tick Ixodes ricinus—A Neglected Public Health Issue in Europe?
Authors: Jaenson Thomas G. T. , Gray Jeremy S. , Lindgren Per-Eric , Wilhelmsson Peter
Published: January 2024

Ixodes ricinus nymphs and adults removed from humans, and larvae and nymphs from birds, have been analysed for infection with Babesia species and Borrelia species previously in separately published studies. Here, we use the same data set to explore the coinfection pattern of Babesia and Borrelia species in the ticks. We also provide an overview of the ecology and potential public health importance in Sweden of I. ricinus infected both with zoonotic Babesia and Borrelia species. Among 1952 nymphs and adult ticks removed from humans, 3.1% were PCR-positive for Babesia spp. Of these Babesia-positive ticks, 43% were simultaneously Borrelia-positive. Among 1046 immatures of I. ricinus removed from birds, 2.5% were Babesia-positive, of which 38% were coinfected with Borrelia species. This study shows that in I. ricinus infesting humans or birds in Sweden, potentially zoonotic Babesia protozoa sometimes co-occur with human-pathogenic Borrelia spp. Diagnostic tests for Babesia spp. infection are rarely performed in Europe, and the medical significance of this pathogen in Europe could be underestimated.
Formulating Parasiticidal Fungi in Dried Edible Gelatins to Reduce the Risk of Infection by Trichuris sp. among Continuous Grazing Bison
Authors: Salmo Rami , Viña Cándido , Zubiria Izaro , Malagón José Ángel Hernández , Sanchís Jaime M. , Cazapal Cristiana , Arias María Sol , Sánchez-Andrade Rita , Paz-Silva Adolfo
Published: January 2024

Control of infection by gastrointestinal nematodes remains a big problem in ruminants under continuous grazing. For the purpose of decreasing the risk of infection by Trichuris sp. in captive bison (Bison bison) always maintained in the same plot, dried gelatins having ≥106 chlamydospores of both Mucor circinelloides and Duddingtonia flagrans were given to them for one week, and at the end, fecal samples (FF) collected each week for four weeks were analyzed immediately. Feces taken one week prior to gelatin administration served as controls (CF). Eggs of Trichuris sp. were sorted into non-viable and viable, then classified into viable undeveloped (VU), viable with cellular development (VCD), or viable infective (VI). Ovistatic and ovicidal effects were determined throughout the study. In FF, viability of Trichuris eggs decreased between 9% (first week) and 57% (fourth week), egg development was delayed during the first two weeks, and VI percentages were significantly lower than in CF (p = 0.001). It is concluded that the preparation of gelatins with chlamydospores of parasiticidal fungi and their subsequent dehydration offer an edible formulation that is ready to use, stress-free to supply, and easy to store, as well as being well-accepted by ruminants and highly efficient to reduce the risk of Trichuris sp. infection among animals under continuous grazing regimes.
Vancomycin AUC-Based Dosing Practices in a Non-Teaching Community Hospital and Associated Outcomes: A One-Year Survey of Uniform Targets for Infections with or without MRSA
Authors: Islam Iftekharul
Published: January 2024

Background: Intravenous (IV) vancomycin area under the curve (AUC)-based dosing is used uniformly for Gram-positive organisms in non-teaching community hospitals. However, evidence for using vancomycin AUC-based dosing for non-methicillin-resistant Staphylococcus aureus (non-MRSA) and less serious infections is limited in the literature. A gap in the literature also exists with respect to comparisons between the outcomes that can be derived using the regimens suggested by Bayesian programs and target doses of the AUC of 400–499 and 500–600. Methods: A retrospective review of all patients hospitalized in a non-teaching community hospital who used AUC-based vancomycin was performed over a 1-year period. Results: Only 17.6% of the included patients had confirmed MRSA. The values for the overall early response rate, 30-day all-cause mortality, and rate of acute kidney injury (AKI) were 50.3%, 11.3%, and 3.8%, respectively, in this population. In regression analysis, compared to non-MRSA infections, a significantly higher rate of early response was seen in patients with MRSA (unadjusted OR = 2.68, 95% CI [1.06–6.76] p = 0.04). Patients in the AUC 400–499 group had a non-significant higher incidence of 30 d mortality and new AKI compared to patients in the AUC 500–600 group. In our Kaplan–Meier survival analysis, there was no statistically significant difference between the comparison groups. Conclusions: Early response was lower in patients with non-MRSA compared to patients with MRSA despite achieving the AUC target. There was no apparent difference in clinical outcomes between the higher and lower AUC groups. Further large-scale research is needed to confirm these findings.
Simulation and Experimental Study on Continuous Wave Fiber Laser Removal of Epoxy Resin Paint Film on the Surface of 6061 Aluminum Alloy
Authors: Li Yahui , Li Jingyi , Dong Hang , Zhang Wei , Jin Guangyong
Published: January 2024

Paint removal is an essential process in the industrial field. Laser technology provides an effective method of paint removal to replace traditional mechanical and chemical methods. This paper establishes a continuous wave (CW) laser thermal paint removal model based on heat conduction theory and Arrhenius’ law. The paint stripping process of epoxy paint film on the surface of 6061 aluminum alloy via CW laser was studied through simulation and experiment. We found that the carbonization of the paint film during the CW laser paint removal process will inhibit the laser paint removal process. Therefore, the paint removal efficiency of the CW laser is limited. The depth of CW laser paint removal increases linearly with the CW laser power density. However, during the CW laser paint removal process, due to the pyrolysis of the paint film and the reflection of the laser by the substrate, the surface temperature of the material first increases and then decreases. In addition, after laser paint removal, the surface roughness of the material after paint removal is reduced due to the melting of the base material. The model established in this article can provide a theoretical reference for studying the CW laser paint removal process.
Terahertz Replica Generation of Ultra-High Data Rate Transmission in an Electro-Optical Semiconductor Optical Amplifier Mach–Zehnder Interferometer System
Authors: Termos Hassan , Mansour Ali
Published: January 2024

This article presents an analysis of an electro-optical up-converter relying on a semiconductor optical amplifier Mach–Zehnder interferometer (SOA-MZI). The pulsed control signal is generated by an optical pulse clock (OPC) with a repetition rate of fs= 19.5 GHz. The intermediate frequency (IF) signal, which carries the modulation format known as quadratic phase shift keying (QPSK) at a frequency fIF, is shifted at the output of the SOA-MZI to high outlet mixing frequencies nfs±fIF, where n represents the harmonic order of the OPC. To examine the characteristics of the sampled QPSK signals, we employ the Virtual Photonics Inc. (VPI) emulator and evaluate them using significate metrics like error vector magnitudes (EVMs), conversion gains, and bit error rates (BERs). The up-mixing process is mainly achieved through the cross-phase modulation (XPM) effect in the SOA-MZI, which operates within a 195.5 GHz ultrahigh frequency (UHF). The electro-optical SOA-MZI up-converter demonstrates consistent uplifting conversion gains across the scope of the output mixing frequencies. The simulated conversion gain deteriorates from 38 dB at 20 GHz to 13 dB at 195.5 GHz. The operational efficiency of the electro-optical SOA-MZI design, employing the standard modulation approach, is also evaluated by measuring the EVM values. The EVM attains a 24% performance level at a data rate of 5 Gbit/s in conjunction with the UHF of 195.5 GHz. To corroborate our results, we compare them with real-world experiments conducted with the UHF of 59 GHz. The maximum frequency range of 1 THz is attained by increasing the OPC repetition rate. Ultimately, through elevating the control frequency to 100 GHz, the generation of terahertz replicas of the 4096-QAM (quadrature amplitude modulation) compound signal becomes achievable at heightened UHF, extending 1 THz, while maintaining a data transmission rate of 120 Gbit/s and upholding exceptional performance characteristics.
Widely Tunable Pulse Duration 100 mJ Single-Mode MOPA System Based on Yb-Doped Tapered Double-Clad Fiber and Nd:YAG Solid-State Amplifiers
Authors: Kozlyakov Mikhail , Petrov Andrey , Mikhailovskiy Grigoriy
Published: January 2024

We report on a 1064 nm master oscillator power amplifier (MOPA) system based on pulse-modulated laser diode seed sources combined with fiber preamplifiers and a Yb-doped tapered double-clad fiber (T-DCF) amplifier used as an all-fiber master oscillator and a two-stage side-pumped solid-state power amplifier. The combination of two master oscillators and a single power amplifier allowed us to obtain pulses with a duration ranging from 10 ns to 10 μs with energy up to 137 mJ at 100 Hz. For the first time, we demonstrate a widely tunable pulse duration and a solid-state MOPA system with over 100 mJ energy based on a T-DCF fiber seed laser.
Design and Optical Performance Analysis of Large-Aperture Optical Windows for Structural Vibration Reduction
Authors: Liu Youchen , Zhang Rui , Han Xiaotao , Wang Qing
Published: January 2024

In order to address the issue of large-aperture optical windows operating in wind tunnel environments with dynamic responses, the damping ratio between the vibration isolation device and the mass of the system was calculated by the passive vibration isolation principle. Two isolation models using circular rubber pads and rectangular rubber pads were proposed, and it was proven that the stiffness value of the circular rubber pad is superior to that of the rectangular rubber pad. A three-dimensional model of the optical window was established using finite element analysis software to simulate the working vibration environment of the optical window. Modal analysis and harmonic response analysis were carried out on the optical system with the isolation device installed, and the nodal data of the optical glass surface changes in the optical window were input into the Zemax 19.4 optical design software in the form of Zernike coefficients to calculate imaging quality evaluation indicators. Through finite element structural analysis of the optical window and evaluation of optical performance indicators, it was demonstrated that under the background of the wind tunnel working environment, the isolation performance of the circular rubber pad in the isolation device of the optical window is superior to that of the rectangular rubber pad. This study can provide a design basis for the isolation analysis methods and isolation measures of optical windows in wind tunnel working environments. These research results have implications for the development of large-aperture optical windows in high-speed wind tunnel applications.
Use of Pseudomonas protegens to Control Root Rot Disease Caused by Boeremia exigua var. exigua in Industrial Chicory (Cichorium intybus var. sativum Bisch.)
Authors: Quezada-D’Angelo Tamara , San Martín Juan , Ruiz Braulio , Oyarzúa Pía , Vargas Marisol , Fischer Susana , Cortés Pamela , Astete Patricio , Moya-Elizondo Ernesto
Published: January 2024

Boeremia exigua var. exigua is a recurrent pathogen causing root rot in industrial chicory. Currently, there is no chemical or varietal control for this disease, and thus, management strategies need to be developed. This study determined the biocontrol effect of strains of Pseudomonas protegens bacteria with antimicrobial compounds on the fungus B. exigua var. exigua under in vitro, in vivo, and field conditions. In addition, root colonization by these bacteria was estimated by the phlD-specific PCR-based dilution end point assay. Eighteen isolates of Pseudomonas spp were evaluated, and the strains that showed the greatest in vitro inhibition of fungal mycelial growth (mm), Ca10A and ChB7, were selected. Inoculation with the strain ChB7 showed less severity (necrotic area) under in vivo conditions (root trials) compared with the control inoculated with the pathogen (p ≤ 0.05). The molecular analysis revealed that the root colonization of plants grown in pots was equal to or greater than 70%. Similar levels were observed in the field trials conducted at the Selva Negra and Canteras experimental stations (2015–2016 season), with values ranging from 85.7 to 70.5% and from 75.0 to 79.5%, respectively. Regarding yield (ton ha−1), values were higher in the treatments inoculated with strains Ca10A and ChB7 (p ≤ 0.05) at both experimental sites, while a lower incidence and severity of root rot were observed at Selva Negra. These results suggest that the Chilean strains of P. protegens are a promising tool for the control of root diseases in industrial chicory.
Evaluation of the RNA Silencing Suppression Activity of Three Cherry Virus F-Encoded Proteins
Authors: Lotos Leonidas , Katsiani Asimina , Katis Nikolaos I. , Maliogka Varvara I.
Published: January 2024

Cherry virus F (CVF) is a newly emerged sweet cherry virus. CVF has been identified in a small number of countries and it has not been associated with discrete symptomatology. RNA silencing is a natural defense mechanism of plants against invaders that degrades viral RNA in a sequence-specific manner. As a counter-defense, plant viruses encode one or more RNA silencing suppressors (RSSs) interfering with the silencing pathway via several mechanisms. To identify putative RSSs, the three proteins (MP, CPL, CPS) encoded by the RNA2 of CVF were selected and separately cloned into the binary vector pART27. The clones were used for transient expression experiments in Nicotiana benthamiana leaves, using co-agroinfiltration with a GFP-expressing vector. In both CPL and CPS, a rapid decrease in fluorescence was recorded, comparable to the negative control, whereas the MP of CVF retained the GFP’s fluorescence for a few days longer even though this was observed in a small number of infiltrated leaves. Further experiments have shown that the protein was not able to inhibit the cell-to-cell spread of the silencing signal; however, a putative interference with systemic silencing was recorded especially when the induction was carried out with double-stranded GFP RNA. Overall, our results indicate that the MP of CVF is putatively implicated in the suppression of RNA silencing, though further experimentation is needed to unveil the exact mode of action.
Interactive Effects of Blue Light and Water Turbulence on the Growth of the Green Macroalga Ulva australis (Chlorophyta)
Authors: Lee Hojun , Depuydt Stephen , Shin Kisik , De Saeger Jonas , Han Taejun , Park Jihae
Published: January 2024

Macroalgal growth and yield are key to sustainable aquaculture. Although light and water turbulence are two important factors that affect algal productivity, research on their interaction is limited. Therefore, in this study, we investigated the effects of different wavelengths of light and the presence or absence of water turbulence on the growth of the green macroalga Ulva australis. Water turbulence was found to enhance the growth of U. australis irrespective of photosynthetic performance, but only in blue light cultures. The quantum dose of blue light required to induce 50% growth promotion was 1.02 mol m−2, which is comparable to the reported values for cryptochrome-mediated effects in other macroalgae. The combined effect of blue light and water turbulence led to the accumulation of photosynthesis-related proteins that support plastid differentiation and facilitate efficient photosynthesis and growth. Our findings thus highlight the potential of harnessing blue light and water turbulence to maximise macroalgal cultivation for sustainable and profitable algal aquaculture.
Chemical Composition of Wild Collected and Cultivated Edible Plants (Sonchus oleraceus L. and Sonchus tenerrimus L.)
Authors: Botella M. Ángeles , Hellín Pilar , Hernández Virginia , Dabauza Mercedes , Robledo Antonio , Sánchez Alicia , Fenoll José , Flores Pilar
Published: January 2024

The present work investigates the nutritional and bioactive composition, as well as the organoleptic and sensory properties, of S. oleraceus and S. tenerrimus, two wild plant species traditionally used in the gastronomy of the Mediterranean area. Additionally, the effect of cultivation on leaf composition was assessed to explore their potential for large-scale production and commercialization from the point of view of possible losses or gains in quality. Both species were characterized as a good source of bioactive compounds, such as vitamins, pro-vitamins and carotenoids, with health-promoting and antioxidant properties that are highly appreciated. The sensory profile revealed a good general acceptance of S. oleraceus and S. tenerrimus, indicating that they could be included in the diet. Although the cultivation of S. oleraceus resulted in a decrease in the concentration of phenolic compounds when compared to wild-harvested plants, the opposite occurred for vitamin C. In S. tenerrimus, cultivation also increased the concentration of other compounds with important nutritional and healthy properties, such as sugars, organic acids and β-carotene. The results of the composition, organoleptic and sensory properties of S. oleraceus and S. tenerrimus support the idea of their potential to be used as edible leafy vegetables and as promising assets for functional foods.
Comprehensive Evaluation of Tomato Growth Status under Aerated Drip Irrigation Based on Critical Nitrogen Concentration and Nitrogen Nutrient Diagnosis
Authors: Lei Hongjun , Fan Yiming , Xiao Zheyuan , Jin Cuicui , Chen Yingying , Pan Hongwei
Published: January 2024

In order to provide a theoretical basis for the rational application of nitrogen fertilizer for tomatoes under aerated drip irrigation, a model of the critical nitrogen dilution curve was established in this study, and the feasibility of the nitrogen nutrition index (NNI) for the real-time diagnosis and evaluation of the nitrogen nutrient status was explored. The tomato variety “FENOUYA” was used as the test crop, and aerated drip irrigation was adopted by setting three levels of aeration rates, namely, A1 (dissolved oxygen concentration of irrigation water is 5 mg L−1), A2 (dissolved oxygen concentration of irrigation water is 15 mg L−1), and A3 (dissolved oxygen concentration of irrigation water is 40 mg L−1), and three levels of nitrogen rates, namely, N1 (120 kg ha−1), N2 (180 kg ha−1) and N3 (240 kg ha−1). The model of the critical nitrogen concentration dilution of tomatoes under different aerated treatments was established. The results showed that (1) the dry matter accumulation of tomatoes increased with the increase in the nitrogen application rate in a certain range and it showed a trend of first increase and then decrease with the increase in aeration rate. (2) As the reproductive period progressed, the nitrogen concentration in tomato plants showed a decreasing trend. (3) There was a power exponential relationship between the critical nitrogen concentration of tomato plant growth and above-ground biomass under different levels of aeration and nitrogen application rate, but the power exponential curves were characterized by A1 (Nc = 15.674DM−0.658), A2 (Nc = 101.116DM−0.455), A3 (Nc = 119.527DM−0.535), N1 (Nc = 33.819DM−0.153), N2 (Nc = 127.759DM−0.555) and N3 (Nc = 209.696DM−0.683). The standardized root mean square error (n-RMSE) values were 0.08%, 3.68%, 3.79% 0.50%, 1.08%, and 0.55%, which were less than 10%, and the model has good stability. (4) The effect of an increased nitrogen application rate on the critical nitrogen concentration dilution curve was more significant than that of the increase in aeration rate. (5) A nitrogen nutrition index model was built based on the critical nitrogen concentration model to evaluate the nitrogen nutritional status of tomatoes, whereby 180 kg ha−1 was the optimal nitrogen application rate, and 15 mg L−1 dissolved oxygen of irrigation water was the optimal aeration rate for tomatoes.
Characterization of the Heat Shock Transcription Factor Family in Lycoris radiata and Its Potential Roles in Response to Abiotic Stresses
Authors: Wang Ning , Shu Xiaochun , Zhang Fengjiao , Song Guowei , Wang Zhong
Published: January 2024

Heat shock transcription factors (HSFs) are an essential plant-specific transcription factor family that regulates the developmental and growth stages of plants, their signal transduction, and their response to different abiotic and biotic stresses. The HSF gene family has been characterized and systematically observed in various species; however, research on its association with Lycoris radiata is limited. This study identified 22 HSF genes (LrHSFs) in the transcriptome-sequencing data of L. radiata and categorized them into three classes including HSFA, HSFB, and HSFC, comprising 10, 8, and 4 genes, respectively. This research comprises basic bioinformatics analyses, such as protein sequence length, molecular weight, and the identification of its conserved motifs. According to the subcellular localization assessment, most LrHSFs were present in the nucleus. Furthermore, the LrHSF gene expression in various tissues, flower developmental stages, two hormones stress, and under four different abiotic stresses were characterized. The data indicated that LrHSF genes, especially LrHSF5, were essentially involved in L. radiata development and its response to different abiotic and hormone stresses. The gene–gene interaction network analysis revealed the presence of synergistic effects between various LrHSF genes’ responses against abiotic stresses. In conclusion, these results provided crucial data for further functional analyses of LrHSF genes, which could help successful molecular breeding in L. radiata.
Applications of Virus-Induced Gene Silencing in Cotton
Authors: Tian Yue , Fang Yao , Zhang Kaixin , Zhai Zeyang , Yang Yujie , He Meiyu , Cao Xu
Published: January 2024

Virus-induced gene silencing (VIGS) is an RNA-mediated reverse genetics technique that has become an effective tool to investigate gene function in plants. Cotton is one of the most important economic crops globally. In the past decade, VIGS has been successfully applied in cotton functional genomic studies, including those examining abiotic and biotic stress responses and vegetative and reproductive development. This article summarizes the traditional vectors used in the cotton VIGS system, the visible markers used for endogenous gene silencing, the applications of VIGS in cotton functional genomics, and the limitations of VIGS and how they can be addressed in cotton.
Nitrogen Deficiency Enhances Eggplant Defense against Western Flower Thrips via the Induction of the Jasmonate Pathway
Authors: Zheng Yueqin , Liu Qianxia , Shi Shuang , Zhu Xiaowen , Chen Yong , Lin Shuo , Tian Houjun , Huang Lanyan , Wei Hui
Published: January 2024

Plant nutrition is connected to defense against insect herbivores, but the exact mechanism underlying the effect of the nitrogen (N) supply on the anti-herbivore capacity of eggplants (Solanum melongena) has not been studied in detail. Therefore, we examined the impact of low (LN, 0.5 mM) and high (HN, 5 mM) nitrate levels on eggplant resistance against the western flower thrips Frankliniella occidentalis (WFT), a major destructive eggplant pest. Our results showed that LN plants displayed enhanced defense responses to WFT compared to HN plants. This included increased transcript levels of key genes in the jasmonic acid (JA) pathway, the accumulation of JA-amido conjugates (jasmonoyl-isoleucine, jasmonoyl-phenylalanine, and jasmonoyl-valine), JA precursor (12-oxophytodienoic acid), and methyl jasmonate, higher transcript levels of defense marker genes (MPK3, MPK7, and WRKY53), and increased activities of polyphenol oxidase and peroxidase upon a WFT attack. Our findings suggest that N deficiency can prime JA-mediated defense responses in eggplants, resulting in increased anti-herbivore resistance.
Structurally Preserved Liquidambar Infructescences, Associated Pollen, and Leaves from the Late Oligocene of the Nanning Basin, South China
Authors: Xu Sheng-Lan , Maslova Natalia , Kodrul Tatiana , Zdravchev Nikita , Kachkina Vasilisa , Liu Xiao-Yan , Wu Xin-Kai , Jin Jian-Hua
Published: January 2024

Liquidambar L. is a significant constituent of the Cenozoic flora in the Northern Hemisphere. Currently, this genus exhibits a discontinuous distribution across Asia and North America, with the center of diversity being in southeastern Asia. This study presents the first occurrence of Liquidambar in the Oligocene of South China. Fossil sweetgum infructescences, associated pollen, and leaves have been found in the Nanning Basin, Guangxi. A new species, Liquidambar nanningensis sp. nov., is described based on the morphological and anatomical characteristics of three-dimensionally preserved infructescences. The Liquidambar fossils from the Nanning Basin show a combination of features indicative of the former genera of Altingiaceae, Altingia, Liquidambar s. str., and Semiliquidambar. The new occurrence expands the taxonomic and morphological diversity of the Paleogene Liquidambar species in South China.