bluuebunny/crossref_metadata_2025 huggingface dataset:
Serological Prevalence of and Risk Factors for Coxiella burnetti Infection in Women of Punjab Province, Pakistan
Authors: Ali Shahzad , Saeed Usama , Rizwan Muhammad , El-Adawy Hosny , Mertens-Scholz Katja , Neubauer Heinrich
Published: April 2022

Background: Coxiella burnetii, the etiological agent of Q (query) fever, provokes abortions in ruminants and is suspected to cause adverse pregnancy outcomes in women. Infection of pregnant women is linked with high mortality and morbidity of the fetus and the mother is at high risk to acquire chronic Q fever. This research was conducted to evaluate the prevalence of Q fever in women and to detect associated risk factors in four districts of Punjab Province, Pakistan. Methods: A total of 297 blood samples were obtained from 147 pregnant and 150 non-pregnant women of the districts Okara, Jhang, Chiniot and Faisalabad of Punjab, Pakistan. Data related to risk factors and demographic parameters were collected using a questionnaire. Serum samples were screened for phase I and phase II specific IgG antibodies for antigens of phase I and phase II using ELISA tests. Univariate and binary regression were used to analyze important risk factors of Q fever. Results: Twenty-five serum samples (8.4%) were found seropositive for Q fever. Seventeen women were positive for Phase-I and twenty-one were positive for phase-II antibodies. Highest and statistically significant (p < 0.05) seroprevalence of 17.1% was observed in Faisalabad. Age, urbanicity, living status, pregnancy status, abortion history, occupation, and consumption of tap water were positively correlated (p < 0.05) with Q fever, while being aged, urbanity, low income, contact with animals and consumption of tap water was identified as potential risk factors. Conclusions: Q fever is prevalent in women of Pakistan. There is a need for an awareness program about the importance of C. burnetii infections and prevention strategies in women during pregnancy to minimize adverse pregnancy outcomes.
Radio Communications on Family Planning: Case of West Africa
Authors: Ahn Jaehyun , Briers Gary , Baker Mathew , Price Edwin , Strong Robert , Piña Manuel , Zickafoose Alexis , Lu Peng
Published: April 2022

Sub-Saharan Africa will accommodate more population this century by having a multitude of births across the continent. Family planning methods provide women with techniques to manage their health and wellbeing. This study investigated how radio communications in family planning changed the perception of Ghanaian, Liberian, and Senegalese mothers toward having fewer children. Univariate and multivariate linear regression results after coarsened exact matching (CEM) with selected covariates for 15- to 49-year-old mothers from demographic and health survey (DHS) data implied the effectiveness of radio communications. This effort supports the need for further research on tailored communication methods for West African mothers over time.
Would Moving Forward Mean Going Back? Comment on Maselli et al. Direct Access to Physical Therapy: Should Italy Move Forward? Int. J. Environ. Res. Public Health 2022, 19, 555
Authors: Moretti Antimo , Costa Massimo , Beretta Giovanna
Published: April 2022

In a recent communication, Maselli and colleagues [...]
A Social Network Analysis of Twitter Data Related to Blood Clots and Vaccines
Authors: Ahmed Wasim , Vidal-Alaball Josep , Vilaseca Josep
Published: April 2022

After the first weeks of vaccination against SARS-CoV-2, several cases of acute thrombosis were reported. These news reports began to be shared frequently across social media platforms. The aim of this study was to conduct an analysis of Twitter data related to the overall discussion. The data were retrieved from 14 March to 14 April 2021 using the keyword ‘blood clots’. A dataset with n = 266,677 tweets was retrieved, and a systematic random sample of 5% of tweets (n = 13,334) was entered into NodeXL for further analysis. Social network analysis was used to analyse the data by drawing upon the Clauset–Newman–Moore algorithm. Influential users were identified by drawing upon the betweenness centrality measure. Text analysis was applied to identify the key hashtags and websites used at this time. More than half of the network comprised retweets, and the largest groups within the network were broadcast clusters in which a number of key users were retweeted. The most popular narratives involved highlighting the low risk of obtaining a blood clot from a vaccine and highlighting that a number of commonly consumed medicine have higher blood clot risks. A wide variety of users drove the discussion on Twitter, including writers, physicians, the general public, academics, celebrities, and journalists. Twitter was used to highlight the low potential of developing a blood clot from vaccines, and users on Twitter encouraged vaccinations among the public.
Salinity as a Determinant Structuring Microbial Communities in Coastal Lakes
Authors: Lew Sylwia , Glińska-Lewczuk Katarzyna , Burandt Paweł , Kulesza Klaudia , Kobus Szymon , Obolewski Krystian
Published: April 2022

The response of bacterioplankton structure to salinity level in coastal lakes (n = 9) along the southern Baltic Sea coastline was studied. In terms of mean salinity levels (0.2–5.2 PSU), the lakes represented freshwater, transitional, and brackish types. Results showed that salinity determines the spatial and seasonal distribution patterns of microorganisms in costal lakes. Increased salinity contributed to a significant decline in total bacterial numbers (TBN). The TBN was lowest in brackish lakes in autumn (4 × 106 cells/mL) and highest in freshwater lakes in summer (7.11 × 106 cells/mL). The groups of Proteobacteria are appropriate bioindicators in any classifications of coastal ecosystems, particularly at low-haline stress. Alpha- and Gamma- subclasses of Proteobacteria are identifiers for brackish habitats, while Betaproteobacteria, due to their intolerance to haline stress, prefer freshwater habitats. Counts of euryhaline Actinobacteria, the dominant group of bacterioplankton (31.8%), decreased significantly with increased salinity. Actinobacteria and Deltaproteobacteria were identifiers of transitional lakes. Cytophaga-Flavobacteria showed affinity with freshwater ecosystems, but this relation was not statistically significant (p > 0.05). The bacteria groups correlated with other physico-chemical parameters of water, such as oxygenation (Actinobacteria) or organic carbon (Betaproteobacteria, Deltaproteobacteria). The impact of hydrological connectivity and salt-water interference on the microbiota structure and biogeochemistry of coastal waters should be considered in the assessment of the ecological status of coastal lakes.
Correlations between Crawl Kinematics and Speed with Morphologic, Functional, and Anaerobic Parameters in Competitive Swimmers
Authors: Strzała Marek , Stanula Arkadiusz , Krężałek Piotr , Sokołowski Kamil , Wądrzyk Łukasz , Maciejczyk Marcin , Karpiński Jakub , Rejdych Wojciech , Wilk Robert , Sadowski Wojciech
Published: April 2022

The purpose of this study was to examine the relationship between a unique complex of predictors and 100 m front crawl race kinematics and swimming speed. In 28 male competitive swimmers (age: 19.6 ± 2.59 years), the following groups of predictors were assessed: (a) the morphologic, (b) the functional upper limb range of motion, and (c) the anaerobic indices of arm-cranking and a series of countermovement jumps. The Pearson product-moment correlation coefficient was calculated to distinguish the predictors and the swimming results. The main finding was that the indices of the power (arm-cranking) and the work (countermovement jump) generated in the anaerobic tests showed a significant and higher correlation with stroke length and stroke index than total body length, upper limb range of motion, or hand and forearm surface area. These results were obtained in accordance with the high swimming economy index relation to clear surface swimming speed. This study reveals that the strength generated by the limbs may represent a predictor of swimming kinematics in a 100 m front crawl performance.
COVID-19 Pandemic Increases the Impact of Low Back Pain: A Systematic Review and Metanalysis
Authors: Papalia Giuseppe Francesco , Petrucci Giorgia , Russo Fabrizio , Ambrosio Luca , Vadalà Gianluca , Iavicoli Sergio , Papalia Rocco , Denaro Vincenzo
Published: April 2022

In March 2019, the World Health Organization (WHO) recognized the COVID-19 pandemic as a global issue. To reduce the spread of this disease, health safety pathways were implemented worldwide. These extraordinary measures changed people’s lifestyles, e.g., by being forced to isolate, and in many cases, to work remotely from home. Low back pain (LBP), the most common cause of disability worldwide, is often a symptom of COVID-19. Moreover, it is often associated with different lifestyle features (type of job, physical activity, body weight). Therefore, the purpose of this systematic review and meta-analysis was to estimate the effect of the COVID-19 lockdown on LBP intensity and prevalence compared with LBP rates before the pandemic. A systematic search was performed on Scopus, PubMed, and Cochrane Central. Overall, eight studies with 2365 patients were included in the analysis. We used the Joanna Briggs Institute (JBI) critical appraisal tool to evaluate the risk of bias: six studies (75%) were at moderate risk of bias and two studies (25%) were at low risk of bias. These studies showed an increase in both the prevalence and intensity of LBP during the COVID-19 lockdown.
Pesticide Exposure in Fruit-Growers: Comparing Levels and Determinants Assessed under Usual Conditions of Work (CANEPA Study) with Those Predicted by Registration Process (Agricultural Operator Exposure Model)
Authors: Bresson Morgane , Bureau Mathilde , Le Goff Jérémie , Lecluse Yannick , Robelot Elsa , Delamare Justine , Baldi Isabelle , Lebailly Pierre
Published: April 2022

Knowledge of pesticide exposure levels in farmers is necessary for epidemiological studies and regulatory purposes. In the European pesticide registration process, operators’ exposure is predicted using the Agricultural Operator Exposure Model (AOEM), created in 2014 by the European Food Safety Authority based on studies conducted by the pesticide industry. We compared operators’ exposures during treatment days in the apple-growing industry under non-controlled working conditions and AOEM-predicted values. The dermal exposure of thirty French apple-growers from the CANEPA study when applying two fungicides was measured using body patches and cotton gloves. For each observation, the corresponding exposure was calculated by means of the AOEM, using data recorded about the operator, spraying equipment and personal protective equipment (PPE) used. A significant linear correlation was observed between calculated and measured daily exposures. The model overestimated the daily exposure approximately 4-fold and the exposure during application 10-fold. However, exposure was underestimated during mixing/loading for 70% of the observations when the operator wore PPE. The AOEM did not overestimate exposures in all circumstances, especially during mixing/loading, when operators handle concentrated products. The protection provided by PPE appeared to be overestimated. This could be due to the optimal working conditions under which the “industrial” studies are conducted, which may not be representative of real working conditions of operators in fruit-growing.
eHealth Engagement on Facebook during COVID-19: Simplistic Computational Data Analysis
Authors: Hermann Caroll , Govender Melanie
Published: April 2022

Understanding social media networks and group interactions is crucial to the advancement of linguistic and cultural behavior. This includes how people accessed advice on health during COVID-19 lockdown. Some people turned to social media to access information on health when other routes were curtailed by isolation rules, particularly among older generations. Facebook public pages, groups and verified profiles using keywords “senior citizen health”, “older generations”, and “healthy living” were analyzed over a 12-month period to examine engagement with social media promoting good mental health. CrowdTangle was used to source status updates, photo and video sharing information in the English language, which resulted in an initial 116,321 posts and 6,462,065 interactions. Data analysis and visualization were used to explore large datasets, including natural language processing for “message” content discovery, word frequency and correlational analysis as well as co-word clustering. Preliminary results indicate strong links to healthy aging information shared on social media, which showed correlations to global daily confirmed cases and daily deaths. The results can identify public concerns early on and address mental health issues among senior citizens on Facebook.
Role of Self-Sampling for Cervical Cancer Screening: Diagnostic Test Properties of Three Tests for the Diagnosis of HPV in Rural Communities of Cuenca, Ecuador
Authors: Vega Crespo Bernardo , Neira Vivian Alejandra , Ortíz Segarra José , Rengel Ruth Maldonado , López Diana , Orellana María Paz , Gómez Andrea , Vicuña María José , Mejía Jorge , Benoy Ina , Parrón Carreño Tesifón , Verhoeven Veronique
Published: April 2022

Background: HPV primary screening has shown effectiveness for cancer prevention; however, gynaecological examination is considered uncomfortable. Self-sampling methods increase the acceptance of screening. The aim of this study is to compare the sensitivity and specificity of clinician sampling versus vaginal and urine self-sampling for HPV diagnosis. Methods: A diagnostic test study was conducted in a rural parish of Cuenca, Ecuador. A total of 120 women participated. Each participant self-collected urine and vaginal samples and underwent clinician sampling for HPV testing. The latter was considered as the golden standard. All three samples were processed with the same amplification and hybridization protocol for HPV detection (Hybribio) following the manufacturer’s instructions. Results: Characteristics of the participants were: median age 35 years; 40.8% married; 46.7% had a primary level of education; and median age of sexual onset, 17.6 years. The prevalence of any type of HPV with clinician sampling was 15.0%, 17.5% with urine sampling and 18.3% with vaginal self-sampling. Self-sampling sensitivity reached 94.4% (IC 74.2–99.9), and specificity 92.1% (IC 85.2–95.9). Urine sampling had a sensitivity of 88.8% (IC 67.2, 96.9), and specificity 94.1% (IC 67.2–96.9). The negative predictive value was 98.9% (IC 94.2–99.8) for vaginal self-sampling and 97.6% (IC 92.6–99.4) for urine sampling. Conclusions: This study shows that vaginal and urine self-sampling methods have similar sensitivity and specificity compared with clinician sampling for the diagnosis of HPV. The correlation between HPV genotypes among the three tests is satisfactory.
Targeting of Mcl-1 Expression by MiRNA-3614-5p Promotes Cell Apoptosis of Human Prostate Cancer Cells
Authors: Hsieh Yi-Hsien , Yu Fang-Jung , Nassef Yasser , Liu Chung-Jung , Chen Yong-Syuan , Lin Ching-Yi , Feng Jia-Liang , Wu Min-Hua
Published: April 2022

MicroRNA (miRNA) acts as a critical regulator of growth in various human malignancies. However, the role of miRNA-3614 in the progression of human prostate cancer remains unknown. In this study, our results demonstrated that miRNA-3614-5p exerts a significant inhibitory effect on cell viability and colony formation and induces sub-G1 cell cycle arrest and apoptosis in human prostate cancer cells. Myeloid cell leukemia-1 (Mcl-1) acts as a master regulator of cell survival. Using the miRNA databases, miRNA-3614-5p was found to regulate Mcl-1 expression by targeting positions of the Mcl-1-3′ UTR. The reduction of Mcl-1 expression by miRNA-3614-5p was further confirmed using an immunoblotting assay. Pro-apoptotic caspase-3 and poly (ADP-ribose) polymerase (PARP) were significantly activated by miRNA-3614-5p to generate cleaved caspase-3 (active caspase-3) and cleaved PARP (active PARP), accompanied by the inhibited Mcl-1 expression. These findings were the first to demonstrate the anti-growth effects of miRNA-3614-5p through downregulating Mcl-1 expression in human prostate cancer cells.
The Importance of CXCL1 in Physiology and Noncancerous Diseases of Bone, Bone Marrow, Muscle and the Nervous System
Authors: Korbecki Jan , Gąssowska-Dobrowolska Magdalena , Wójcik Jerzy , Szatkowska Iwona , Barczak Katarzyna , Chlubek Mikołaj , Baranowska-Bosiacka Irena
Published: April 2022

This review describes the role of CXCL1, a chemokine crucial in inflammation as a chemoattractant for neutrophils, in physiology and in selected major non-cancer diseases. Due to the vast amount of available information, we focus on the role CXCL1 plays in the physiology of bones, bone marrow, muscle and the nervous system. For this reason, we describe its effects on hematopoietic stem cells, myoblasts, oligodendrocyte progenitors and osteoclast precursors. We also present the involvement of CXCL1 in diseases of selected tissues and organs including Alzheimer’s disease, epilepsy, herpes simplex virus type 1 (HSV-1) encephalitis, ischemic stroke, major depression, multiple sclerosis, neuromyelitis optica, neuropathic pain, osteoporosis, prion diseases, rheumatoid arthritis, tick-borne encephalitis (TBE), traumatic spinal cord injury and West Nile fever.
The Role of Inflammasomes in Glomerulonephritis
Authors: Anton-Pampols Paula , Diaz-Requena Clara , Martinez-Valenzuela Laura , Gomez-Preciado Francisco , Fulladosa Xavier , Vidal-Alabro Anna , Torras Joan , Lloberas Núria , Draibe Juliana
Published: April 2022

The inflammasome is an immune multiprotein complex that activates pro-caspase 1 in response to inflammation-inducing stimuli and it leads to IL-1β and IL-18 proinflammatory cytokine production. NLRP1 and NLRP3 inflammasomes are the best characterized and they have been related to several autoimmune diseases. It is well known that the kidney expresses inflammasome genes, which can influence the development of some glomerulonephritis, such as lupus nephritis, ANCA glomerulonephritis, IgA nephropathy and anti-GBM nephropathy. Polymorphisms of these genes have also been described to play a role in autoimmune and kidney diseases. In this review, we describe the main characteristics, activation mechanisms, regulation and functions of the different inflammasomes. Moreover, we discuss the latest findings about the role of the inflammasome in several glomerulonephritis from three different points of view: in vitro, animal and human studies.
Non-Coding RNAs Regulate Spontaneous Abortion: A Global Network and System Perspective
Authors: Gan Jianyu , Gu Ting , Yang Huaqiang , Ao Zheng , Cai Gengyuan , Hong Linjun , Wu Zhenfang
Published: April 2022

Spontaneous abortion is a common pregnancy complication that negatively impacts women’s health and commercial pig production. It has been demonstrated that non-coding RNA (ncRNA) is involved in SA by affecting cell proliferation, invasion, apoptosis, epithelial-mesenchymal transformation (EMT), migration, and immune response. Over the last decade, research on ncRNAs in SA has primarily concentrated on micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs). In this review, we discuss recent ncRNA studies focused on the function and mechanism of miRNAs, lncRNAs, and circRNAs in regulating SA. Meanwhile, we suggest that a ceRNA regulatory network exists in the onset and development of SA. A deeper understanding of this network will accelerate the process of the quest for potential RNA markers for SA diagnosis and treatment.
Long-Term Fluorescent Tissue Marking Using Tissue-Adhesive Porphyrin with Polycations Consisting of Quaternary Ammonium Salt Groups
Authors: Komatsu Yoshiki , Yoshitomi Toru , Furuya Kinji , Ikeda Takafumi , Terasaki Azusa , Hoshi Aoi , Kawazoe Naoki , Chen Guoping , Matsui Hirofumi
Published: April 2022

Localization of tumors during laparoscopic surgery is generally performed by locally injecting India ink into the submucosal layer of the gastrointestinal tract using endoscopy. However, the location of the tumor is obscured because of the black-stained surgical field and the blurring caused by India ink. To solve this problem, in this study, we developed a tissue-adhesive porphyrin with polycations consisting of quaternary ammonium salt groups. To evaluate the ability of tissue-adhesive porphyrin in vivo, low-molecular-weight hematoporphyrin and tissue-adhesive porphyrin were injected into the anterior wall of the exposed stomach in rats. Local injection of low-molecular-weight hematoporphyrin into the anterior wall of the stomach was not visible even after 1 day because of its rapid diffusion. In contrast, the red fluorescence of the tissue-adhesive porphyrin was visible even after 7 days due to the electrostatic interactions between the positively-charged moieties of the polycation in the tissue-adhesive porphyrin and the negatively-charged molecules in the tissue. In addition, intraperitoneal injection of tissue-adhesive porphyrin in rats did not cause adverse effects such as weight loss, hepatic or renal dysfunction, or organ adhesion in the abdominal cavity. These results indicate that tissue-adhesive porphyrin is a promising fluorescent tissue-marking agent.
Mebendazole-Induced Blood-Testis Barrier Injury in Mice Testes by Disrupting Microtubules in Addition to Triggering Programmed Cell Death
Authors: Huang Mingqian , Wang Chang , Yao Ying , Li Huiling , Yao Yejin , Zhu Yunfei , Cui Yiqiang , Yuan Yan , Sha Jiahao
Published: April 2022

Mebendazole (MBZ) is a synthetic benzimidazole known for its antiparasitic properties. In recent years, growing evidence showed that MBZ was also used as an anti-tumor agent. However, whether (and to what extent) this drug treatment affected the male reproductive system was not well-understood. In this study, male C57BL/6 mice were injected with 40 mg/kg/day of MBZ. The treatment was for 3 and 7 days. Our results showed that the injected mice exhibited an abnormal spermatogenic phase with a significant decrease in sperm. We further detected microtubule disruption and transient functional destruction of the blood–testes barrier (BTB) in the MBZ-injected mice testes (BTB). Our data confirmed that MBZ suppressed the expression of the BTB junction-associated proteins and disrupted the Sertoli cells’ function in vivo. Moreover, MBZ-treated mice demonstrated an aberrant caspase-3 signalling pathway, which resulted in the apoptosis of the germ cells. Here, we present our data, indicating that MBZ impairs BTB by reducing the expression of the microtubules’ and BTB junction-associated proteins. The last leads to activating the caspase-3 pathway, which triggers extensive germ cell apoptosis.
The Combination of Conventional QTL Analysis, Bulked-Segregant Analysis, and RNA-Sequencing Provide New Genetic Insights into Maize Mesocotyl Elongation under Multiple Deep-Seeding Environments
Authors: Zhao Xiaoqiang , Niu Yining
Published: April 2022

Mesocotyl length (MES) is an important trait that affects the emergence of maize seedlings after deep-seeding and is closely associated with abiotic stress. The elucidation of constitutive-QTLs (cQTLs) and candidate genes for MES and tightly molecular markers are thus of great importance in marker-assisted selection (MAS) breeding. Therefore, the objective of this study was to perform detailed genetic analysis of maize MES across 346 F2:3 families, 30/30 extreme bulks of an F2 population, and two parents by conventional QTL analysis, bulked-segregation analysis (BSA), and RNA-sequencing when maize was sown at the depths of 3, 15, and 20 cm, respectively. QTL analysis identified four major QTLs in Bin 1.09, Bin 3.04, Bin 4.06–4.07, and Bin 6.01 under two or more environments, which explained 2.89–13.97% of the phenotypic variance within a single environment. BSA results revealed the presence of seven significantly linked SNP/InDel regions on chromosomes 1 and 4, and six SNP/InDel regions and the major QTL of qMES4-1 overlapped and formed a cQTL, cQMES4, within the 160.98–176.22 Mb region. In total, 18,001 differentially expressed genes (DEGs) were identified across two parents by RNA-sequencing, and 24 of these genes were conserved core DEGs. Finally, we validated 15 candidate genes in cQMES4 to involve in cell wall structure, lignin biosyntheis, phytohormones (auxin, abscisic acid, brassinosteroid) signal transduction, circadian clock, and plant organ formation and development. Our findings provide a basis for MAS breeding and enhance our understanding of the deep-seeding tolerance of maize.
A Leucyl-tRNA Synthetase Urzyme: Authenticity of tRNA Synthetase Catalytic Activities and Promiscuous Phosphorylation of Leucyl-5′AMP
Authors: Hobson Jessica J. , Li Zhijie , Hu Hao , Carter Charles W.
Published: April 2022

Aminoacyl-tRNA synthetase (aaRS)/tRNA cognate pairs translate the genetic code by synthesizing specific aminoacyl-tRNAs that are assembled on messenger RNA by the ribosome. Deconstruction of the two distinct aaRS superfamilies (Classes) has provided conceptual and experimental models for their early evolution. Urzymes, containing ~120–130 amino acids excerpted from regions where genetic coding sequence complementarities have been identified, are key experimental models motivated by the proposal of a single bidirectional ancestral gene. Previous reports that Class I and Class II urzymes accelerate both amino acid activation and tRNA aminoacylation have not been extended to other synthetases. We describe a third urzyme (LeuAC) prepared from the Class IA Pyrococcus horikoshii leucyl-tRNA synthetase. We adduce multiple lines of evidence for the authenticity of its catalysis of both canonical reactions, amino acid activation and tRNALeu aminoacylation. Mutation of the three active-site lysine residues to alanine causes significant, but modest reduction in both amino acid activation and aminoacylation. LeuAC also catalyzes production of ADP, a non-canonical enzymatic function that has been overlooked since it first was described for several full-length aaRS in the 1970s. Structural data suggest that the LeuAC active site accommodates two ATP conformations that are prominent in water but rarely seen bound to proteins, accounting for successive, in situ phosphorylation of the bound leucyl-5′AMP phosphate, accounting for ADP production. This unusual ATP consumption regenerates the transition state for amino acid activation and suggests, in turn, that in the absence of the editing and anticodon-binding domains, LeuAC releases leu-5′AMP unusually slowly, relative to the two phosphorylation reactions.
Multifunctional Role of Lipids in Modulating the Tumorigenic Properties of 4T1 Breast Cancer Cells
Authors: He Yuanyuan , Rezaei Somayeh , Júnior Raimundo Fernandes de Araújo , Cruz Luis J. , Eich Christina
Published: April 2022

Tumor growth and progression are linked to an altered lipid metabolism in the tumor microenvironment (TME), including tumor cells and tumor-associated macrophages (TAMs). A growing number of lipid metabolism targeting drugs have shown efficacy in anti-tumor therapy. In addition, exogenously applied lipids and lipid analogues have demonstrated anti-tumor activities in several cancers, including breast cancer. In this study, we investigated the anti-tumor efficacies of the natural lipids palmitic acid (PA), sphingomyelin (SM), ceramide (Cer) and docosahexaenoic acid (DHA) on breast cancer cells. All tested lipids reduced the malignancy of breast cancer cells in vitro by impairing cell proliferation, migration and invasiveness. PA showed superior anti-tumor properties, as it additionally impaired cancer cell viability by inducing apoptosis, without affecting healthy cells. Co-culture experiments further demonstrated that Cer and PA reduced the immunosuppressive phenotype of M2 macrophages and the M2 macrophage-promoted the epithelial–mesenchymal transition (EMT) and migration of breast cancer cells. At the molecular level, this coincided with the up-regulation of E-cadherin. Our results highlight a powerful role for exogenously applied PA and Cer in reducing breast cancer tumorigenicity by simultaneously targeting cancer cells and M2 macrophages. Our findings support the notion that lipids represent alternative biocompatible therapeutic agents for breast cancer.
Molecular Traits and Functional Exploration of BES1 Gene Family in Plants
Authors: Sun Zhenting , Liu Xingzhou , Zhu Weidong , Lin Huan , Chen Xiugui , Li Yan , Ye Wuwei , Yin Zujun
Published: April 2022

The BES1 (BRI1-EMSSUPPRESSOR1) gene family is a unique class of transcription factors that play dynamic roles in the Brassinosteroids (BRs) signaling pathway. The published genome sequences of a large number of plants provide an opportunity to identify and perform a comprehensive functional study on the BES1 gene family for their potential roles in developmental processes and stress responses. A total of 135 BES1 genes in 27 plant species were recognized and characterized, which were divided into five well-conserved subfamilies. BES1 was not found in lower plants, such as Cyanophora paradoxa and Galdieria sulphuraria. The spatial expression profiles of BES1s in Arabidopsis, rice, and cotton, as well as their response to abiotic stresses, were analyzed. The overexpression of two rice BES1 genes, i.e., OsBES1-3 and OsBES1-5, promotes root growth under drought stress. The overexpression of GhBES1-4 from cotton enhanced the salt tolerance in Arabidopsis. Five protein interaction networks were constructed and numerous genes co-expressed with GhBES1-4 were characterized in transgenic Arabidopsis. BES1 may have evolved in the ancestors of the first land plants following its divergence from algae. Our results lay the foundation for understanding the complex mechanisms of BES1-mediated developmental processes and abiotic stress tolerance.
Roles of Nitric Oxide in Brain Ischemia and Reperfusion
Authors: Wang Yijie , Hong Fenfang , Yang Shulong
Published: April 2022

Brain ischemia and reperfusion (I/R) is one of the most severe clinical manifestations of ischemic stroke, placing a significant burden on both individuals and society. The only FDA-approved clinical treatment for ischemic stroke is tissue plasminogen activator (t-PA), which rapidly restores cerebral blood flow but can have severe side effects. The complex pathological process of brain I/R has been well-established in the past few years, including energy metabolism disorders, cellular acidosis, doubling of the synthesis or release of excitotoxic amino acids, intracellular calcium homeostasis, free radical production, and activation of apoptotic genes. Recently, accumulating evidence has shown that NO may be strongly related to brain I/R and involved in complex pathological processes. This review focuses on the role of endogenous NO in pathological processes in brain I/R, including neuronal cell death and blood brain barrier disruption, to explore how NO impacts specific signaling cascades and contributes to brain I/R injury. Moreover, NO can rapidly react with superoxide to produce peroxynitrite, which may also mediate brain I/R injury, which is discussed here. Finally, we reveal several therapeutic approaches strongly associated with NO and discuss their potential as a clinical treatment for ischemic stroke.
Differences in Cardiac Etiologies for Ischemic Stroke in Young and Middle-Aged Patients: A Single-Center Experience in Taiwan
Authors: Wang Wen-Hwa
Published: April 2022

Background: The cardiac etiology of acute ischemic stroke (AIS) plays an important role in young adults; therefore, complete cardiac workups and subsequent prevention methods are important for treating young AIS patients. However, the definition of a young age for AIS patients could be below 45 years old, while in some previous studies, it has been below 55 years old. It remains unclear whether cardiac workups are equally important for AIS patients in the young (the age of 20–45 years old) and middle-aged (46–55 years old) categories. Materials and methods: This prospective study included 103 patients admitted due to a first AIS attack younger than 55 years old during the period from 1 October 2018 to 31 December 2020. All the patients received cardiologist consultations and cardiac workups accordingly. The characteristics of patients, cardiac workups, clinical findings, and management were analyzed. Results: AIS patients in the 46–55-year-old group had a higher prevalence of hypertension (61.4% vs. 39.0%), diabetes mellitus (27.3% vs. 15.3%), a previous history of coronary artery disease (9.1% vs. 1.7%), and atrial fibrillation (9.1% vs. 1.7%) compared to the 20–45-year-old group. After cardiologist consultations, a higher prevalence of newly diagnosed coronary artery disease (6.8% vs. 1.7%) and congestive heart failure (11.4% vs. 1.7%) was noted. Both groups disclosed similar percentages of patent foramen ovale (PFO) (27.3% vs. 22.0%) and valvular disease. These results led to subsequent changes in treatment in both groups. The 20–45-year-old group had higher percentages of receiving PFO occluders (11.9%) compared to the 46–55-year-old group (6.8%). Conclusion: Cardiologist consultations with thorough cardiac workups for AIS patients can reveal many cardiac findings in both young and middle-aged patients. This leads to a subsequent change in treatment, including medical and surgical aspects, which are important as secondary prevention for AIS.
Glass Frit Jetting for Advanced Wafer-Level Hermetic Packaging
Authors: Roshanghias Ali , Bardong Jochen , Binder Alfred
Published: April 2022

Glass frit bonding is a widely used technology to cap and seal micro-electromechanical systems on the wafer level using a low melting point glass. Screen printing is the main method to apply glass frit paste on wafers. Screen printing of glass frit paste is usually performed on less sensitive, less critical wafers, normally the capping wafer, because screen printing is a rough process involving the mechanical contact of the screen printing mesh and the wafer. However, for some applications in which contactless patterning of glass frit materials on the device wafers are preferred (e.g., 3D topographies, micro-lens and optics integration) jet dispensing could be a promising approach. Consequently, in this study, wafer-level jetting of glass frit materials on silicon wafers was proposed and investigated. The jetting parameters such as jetting distance, power and temperature were optimized for a glass frit paste. Additionally, the effect of jetted pitch size on the bond-line thickness was assessed. The wafers with jetted glass frit pastes were conclusively bonded in low vacuum and characterized. As a single-step (non-contact) additive approach, the jet printing of glass frit was revealed to be a straightforward, cost-effective and flexible approach with several implications for hermetic packaging.
Heterostructured Bi2O3@rGO Anode for Electrochemical Sodium Storage
Authors: Hai Benrong , Liu Changsheng
Published: April 2022

Bismuth oxide (Bi2O3) is an auspicious anode material for sodium-ion batteries owing to its high theoretical capacity and abundant Bi resources. However, the poor electronic conductivity and huge volume expansion of Bi2O3 during cycling lead to the low coulombic efficiency and unstable cycling stability. Aiming to suppress these issues, we use highly conductive reduced graphene oxide (rGO) as a continuous skeleton to fabricate a Bi2O3@rGO heterostructure. It exhibits high reversibility and stability for electrochemical sodium storage by delivering a reversible capacity of 161 mAh g−1 after 100 cycles at 50 mA g−1, which completely outperforms Bi2O3 (43 mAh g−1). In addition, the coulombic efficiency of the heterostructure stabilizes at >90% upon only 3 cycles. The results can be attributed to the dual function of rGO in supporting Bi2O3 nanoparticles and providing conductive pathways to fasten electron transport.
Study on the Strength and Hydration Behavior of Sulfate-Resistant Cement in High Geothermal Environment
Authors: Wang Yan , Chen Yahao , Guo Bingbing , Zhang Shaohui , Tong Yueping , Niu Ditao
Published: April 2022

The hydration process and compressive strength and flexural strength development of sulphate-resistant Portland cement (SRPC) curing at 20 °C, 40 °C, 50 °C, and 60 °C were studied. In addition, MIP, XRD, SEM, and a thermodynamic simulation (using Gibbs Energy Minimization Software (GEMS)) were used to study the pore structure, the types, contents, and transformations of hydration products, and the changes in the internal micro-morphology. The results indicate that, compared with normal-temperature curing (20 °C), the early compressive strength (1, 3, and 7 d) of SRPC cured at 40~60 °C increased by 10.1~57.4%, and the flexural strength increased by 1.8~21.3%. However, high-temperature curing was unfavorable for the development of compressive strength and flexural strength in the later period (28~90 d), as they were reduced by 1.5~14.6% and 1.1~25.5%, respectively. With the increase in the curing temperature and curing age, the internal pores of the SRPC changed from small pores to large pores, and the number of harmful pores (>50 nm) increased significantly. In addition, the pore structure was further coarsened after curing at 60 °C for 90 d, and the number of multiple harmful pores (>200 nm) increased by 17.9%. High-temperature curing had no effect on the types of hydration products of the SRPC but accelerated the formation rate of hydration products. The production of the hydration products C-S-H increased by 13.5%, 18.6%, and 22.8% after curing at 40, 50, and 60 °C for 3 d, respectively. The stability of ettringite (AFt) reduced under high-temperature curing, and its diffraction peak was not observed in the XRD patterns. When the curing temperature was higher than 50 °C, AFt began to transform into monosulfate, which consumed more tricalcium aluminate hydrate and inhibited the formation of “delayed ettringite”. Under high-temperature curing, the compactness of the internal microstructure of the SRPC decreased, and the distribution of hydration products was not uniform, which affected the growth in its strength during the later period.
The Influence of Material Type and Hardness on the Number of Embedded Abrasive Particles during Airborne-Particle Abrasion
Authors: Smielak Beata , Klimek Leszek
Published: April 2022

(1) Background: This paper aims to determine the influence of hardness on the number of abrasive material grains (SiC) embedded on the surface metal alloys and ZrO2 during abrasion. (2) Methods: Cylindrical samples were created: 315 made of Cr/Co, Ni/Cr or Ti, and 315 made of sintered ZrO2- 3TPZ-Y. These were divided into four groups (each n = 35 samples), and were treated with SiC grain sizes 50, 110, and 250 µm at pressures 0.2, 0.4, or 0.6 MPa. The samples were then observed in SEM to study SE and BSE. The surface coverage of abrasive material particles was determined by quantitative metallography. Five samples from each group were subjected to hardness measurements. The results were compared with three-factor variance analysis with using the post hoc Tukey test. (3) Results: The highest amount (40.06) of embedded abrasive was obtained for Ti alloy with a gradation of 250µm at a pressure of 0.6 MPa. The smallest amount of embedded grain (2.66) was obtained for ZrO2 for the same treatment parameters. (4) Conclusions: The amount of embedded abrasive particles depends on the type of treated material, gradation particles, and the amount of applied pressure. Harder treated materials are more resistant to grains of abrasive becoming embedded on surface.
The Effect of Low-Processing Temperature on the Physicochemical and Mechanical Properties of Bovine Hydroxyapatite Bone Substitutes
Authors: Abdelmoneim Dina , Porter Gemma Claire , Coates Dawn Elizabeth , Duncan Warwick John , Waddell John Neil , Hammer Niels , Li Kai Chun
Published: April 2022

Bovine bone grafts (BBX) require protein removal as part of the manufacturing process to reduce antigenicity and, in consequence, to be safely used in humans. Deproteinisation may have direct effects on the characteristics of the bone material and on in vivo material performance. This research aimed to comprehensively study the physicochemical and mechanical properties of BBX processed at low deproteinisation processing temperatures. Cubes of bovine bone (8 mm3) were treated with temperatures between 100 °C and 220 °C at 30 °C intervals and with pressures ranging from 1.01 to 24.58 Bar. The samples were characterised topographically and mechanically using scanning electron microscopy (SEM), atomic force microscopy (AFM), and uniaxial bending tests. The organic content and the chemical composition were determined using thermogravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FTIR). X-ray diffraction (XRD) and FTIR were also used to quantitatively determine the specimen crystallinity. Increasing temperature/pressure was associated with decreasing protein levels and compressive strength and increasing surface irregularities and crystallinity. The findings suggest that low-temperature processed bone is likely to exhibit a rapid in vivo degradation rate. The deproteinisation temperature can be adjusted to tailor the graft properties for specific applications.
Crack Initiation Mechanism and Life Prediction of Ti60 Titanium Alloy Considering Stress Ratios Effect in Very High Cycle Fatigue Regime
Authors: He Ruixiang , Peng Haotian , Liu Fulin , Khan Muhammad Kashif , Chen Yao , He Chao , Wang Chong , Wang Qingyuan , Liu Yongjie
Published: April 2022

Ultrasonic fatigue tests were performed on Ti60 titanium alloy up to a very high cycle fatigue (VHCF) regime at various stress ratios to investigate the characteristics. The S-N curves showed continuous declining trends with fatigue limits of 400, 144 and 130 MPa at 109 cycles corresponding to stress ratios of R = −1, 0.1 and 0.3, respectively. Fatigue cracks found to be initiated from the subsurface of the specimens in the VHCF regime, especially at high stress ratios. Two modified fatigue life prediction models based on fatigue crack initiation mechanisms for Ti60 titanium alloy in the VHCF regime were developed which showed good agreement with the experimental data.
Role of Bimetallic Solutions in the Growth and Functionality of Cu-BTC Metal–Organic Framework
Authors: Gupta Nishesh Kumar , Bae Jiyeol , Kim Kwang-Soo
Published: April 2022

Bimetallic solutions play a vital role in the growth and functionality of copper trimesate (Cu-BTC) metal–organic frameworks (MOFs). The effect of Ag+, Ca2+, Mn2+, Co2+, and Zn2+ on the growth of Cu-BTC was studied by fabricating M-Cu-BTC MOFs at room temperature using bimetallic M-Cu solutions. While Ag+ in the MOF had a rod-like morphology and surface properties, divalent cations deteriorated it. Moreover, unconventional Cu+ presence in the MOF formed a new building unit, which was confirmed in all the MOFs. Apart from Ag and Mn, no other MOF showed any presence of secondary cations in the structure. While Ag-Cu-BTC showed an improved H2S uptake capacity, other M-Cu-BTC MOFs had superior organic pollutant adsorption behavior. Thus, we have demonstrated that the physicochemical properties of Cu-BTC could be modified by growing it in bimetallic solutions.
Electrochemical Self-Assembled Gold Nanoparticle SERS Substrate Coupled with Diazotization for Sensitive Detection of Nitrite
Authors: Han En , Zhang Maoni , Pan Yingying , Cai Jianrong
Published: April 2022

The accurate determination of nitrite in food samples is of great significance for ensuring people’s health and safety. Herein, a rapid and low-cost detection method was developed for highly sensitive and selective detection of nitrite based on a surface-enhanced Raman scattering (SERS) sensor combined with electrochemical technology and diazo reaction. In this work, a gold nanoparticle (AuNP)/indium tin oxide (ITO) chip as a superior SERS substrate was obtained by electrochemical self-assembled AuNPs on ITO with the advantages of good uniformity, high reproducibility, and long-time stability. The azo compounds generated from the diazotization-coupling reaction between nitrite, 4-aminothiophenol (4-ATP), and N-(1-naphthyl) ethylenediamine dihydrochloride (NED) in acid condition were further assembled on the surface of AuNP/ITO. The detection of nitrite was realized using a portable Raman spectrometer based on the significant SERS enhancement of azo compounds assembled on the AuNP/ITO chip. Many experimental conditions were optimized such as the time of electrochemical self-assembly and the concentration of HAuCl4. Under the optimal conditions, the designed SERS sensor could detect nitride in a large linear range from 1.0 × 10−6 to 1.0 × 10−3 mol L−1 with a low limit of detection of 0.33 μmol L−1. Additionally, nitrite in real samples was further analyzed with a recovery of 95.1−109.7%. Therefore, the proposed SERS method has shown potential application in the detection of nitrite in complex food samples.
Tuning of Magnetic Damping in Y3Fe5O12/Metal Bilayers for Spin-Wave Conduit Termination
Authors: Krysztofik Adam , Kuznetsov Nikolai , Qin Huajun , Flajšman Lukáš , Coy Emerson , van Dijken Sebastiaan
Published: April 2022

In this work, we investigate the structural and dynamic magnetic properties of yttrium iron garnet (YIG) films grown onto gadolinium gallium garnet (GGG) substrates with thin platinum, iridium, and gold spacer layers. Separation of the YIG film from the GGG substrate by a metal film strongly affects the crystalline structure of YIG and its magnetic damping. Despite the presence of structural defects, however, the YIG films exhibit a clear ferromagnetic resonance response. The ability to tune the magnetic damping without substantial changes to magnetization offers attractive prospects for the design of complex spin-wave conduits. We show that the insertion of a 1-nm-thick metal layer between YIG and GGG already increases the effective damping parameter enough to efficiently absorb spin waves. This bilayer structure can therefore be utilized for magnonic waveguide termination. Investigating the dispersionless propagation of spin-wave packets, we demonstrate that a damping unit consisting of the YIG/metal bilayers can dissipate incident spin-wave signals with reflection coefficient R < 0.1 at a distance comparable to the spatial width of the wave packet.
Temperature Distribution in the Flow of a Viscous Incompressible Non-Newtonian Williamson Nanofluid Saturated by Gyrotactic Microorganisms
Authors: Areshi Mounirah , Alrihieli Haifaa , Alali Elham , Megahed Ahmed M.
Published: April 2022

The heat and mass transfer in magnetized non-Newtonian Williamson nanofluid flow, saturated by gyrotactic microorganisms due to a stretched sheet, is debated here. The rough sheet is subjected to uniform heat flux, and its velocity is proportional to its distance from the slit. Nanofluid viscosity and thermal conductivity are temperature-dependent, but microbe diffusivity and Brownian motion are concentration-dependent. Through similarity transformation, the system of modeled equations is reduced to dimensionless differential equations. We employed the shooting approach in conjunction with the Runge–Kutta scheme to obtain a solution for the physical model. For various combinations of the controlling parameters, some numerical results are found. When the generated results are compared to the existing literature, the highest settlement is found. According to numerical results, the skin-friction coefficient rises as the magnetic field and thermal conductivity parameters rise, while the opposite tendency is observed for both the slip velocity and viscosity parameters.