bluuebunny/crossref_metadata_2025 huggingface dataset:
Country-wide, multi-location trails of Green Super Rice lines for yield performance and stability in Pakistan using genetic and stability parameters
Authors: Ahmed Muhammad Shahzad , Majeed Abid , Attia Kotb A. , Javaid Rana Arsalan , Siddique Faiza , Farooq Muhammad Shahbaz , Uzair Muhammad , Abushady Asmaa M.
Published: September 2023

Background: Rice (Oryza sativa L.) is an important member of the family Poaceae and more than half of world population dependent for their dietary nutrition on rice. Rice cultivars with high yield, resilient to stresses and wider environmental adoptability are essential to ensure the yield stability and food security. The fundamental objective of this study was to identify yielding rice genotypes with stability performance and wider adoptability in a rice growing areas in Pakistan.
Methodology & Results: A triplicate RCBD design experiment containing 20 Green Super Rice (GSR) advanced lines was conducted at 12 rice growing ecologies in four Provinces in Pakistan. Grain yield stability performance was assessed by using mean performance, analysis of variance (ANOVA), genetic components (genotypic, phenotypic, environmental variances and heritability), univariate and multivariate statistics i.e. regression coefficient, AMMI stability value, AMMI stability index, Wricke’s Ecovalence, Shukla’s Stability Variance and Weighted Average of Absolute Score, AMMI model and GGE biplot analysis respectively. Analysis of variance revealed significant differences among genotypes, locations, and G x E interaction for mean squares (p < 0.05) of PH, NT, PL, GPP, TGW and PY. all the traits except NT revealed higher genotypic variance than environmental variance. Broad sense heritability was estimated in the range of 44.36% to 98.60%. Based on ASV, ASI, bi, Wi2, σ2i and WAAS statistics, the genotypes G1, G4, G5, G8, G11 and G12 revealed lowest values for parametric statistics and considered more stable genotypes on the bases of paddy yield. The additive main effects and multiplicative interaction (AMMI) model revealed significant variation (p< 0.05) for genotypes and non-signification for environment and highly significant for G × E interaction. The variation proportion of PC1 and PC2 from interaction revealed 67.2% variability for paddy yield. Based on ‘mean verses stability analysis of GGE biplot’, ‘Which-won-where’ GGE Biplot, ‘discriminativeness vs. representativeness’ pattern of stability, ‘IPCA and WAASB/GY’ ratio-based stability Heat-map, and ranking of genotypes, the genotypes G1, G2, G3, G5, G8, G10, G11 and G13 were considered best performing and stable genotypes with yield potential more than 8 tons ha-1. Discriminativeness vs. representativeness’ pattern of stability identifies two environments, E5 (D.I Khan, KPK) and E6 (Usta Muhammad, Baluchistan) were best suited for yield performance.
Conclusions: Based on these findings we have concluded that the genotypes (G1, G2, G3, G5, G8, G10, G11 and G13) can be recommended for commercial variety development process and future breeding program.
Implementing routine paediatric height/length and weight screening and weight management advice by clinicians: An evaluation
Authors: Krstic Slavica , Dennis Sarah , Southcombe Faye , Denney-Wilson Elizabeth
Published: September 2023

OBJECTIVE: To determine the views of health care professionals(HCPs) in South Western Sydney Local Health District (SWSLHD) about the effectiveness of implementation strategies used to increase routine height/length and weight screening, advice, and referral for children and adolescents. A secondary aim was to explore the prevalence of weight bias among HCPs.
METHODS: A questionnaire was sent to all HCPs who had undertaken online or face-to-face training between December 2018 and June 2020 in SWSLHD (n=840). The questionnaire collected data on their experience of routine height and weight screening and the effectiveness of strategies used in the implementation. It also included a weight bias assessment. Data were provided by the NSW Ministry of Health on the performance of routine height/length and weight measures entered into the electronic medical records (eMR) in SWSLHD.
RESULTS:  Of the 840 questionnaires sent, 87 were undeliverable; of the remaining 753, 285 were returned (38% response rate). More than half (53%, 151/285) of the participants were nurses. Most HCPs agreed that there was a need for routine screening and reported that education, training, and access to resources were the most helpful implementation strategies. Most HCPs were confident in performing routine screening but were less confident in raising the issue of weight with children and their families. Barriers to implementation were lack of time, equipment, appropriate clinical setting, and HCPs’ perceptions and beliefs about obesity.
CONCLUSION: Routine screening is the first step in identifying children and adolescents at risk of overweight and obesity, but many HCP found it challenging to incorporate into daily practice. Multifaceted strategies are effective in increasingroutine screening across diverse healthcare settings so that children and adolescents receive timely and appropriate intervention.
Advancing SSP-aligned Scenarios of Shipping Toward 2050
Authors: Kramel Diogo , Franz Sebastian Marco , Klenner Jan , Muri Helene , Münster Marie , Strømman Anders Hammer
Published: September 2023

The wide range of projections for shipping poses a challenge for modeling the sector in Integrated Assessment Models (affecting how attainable decarbonization is in the sector) and Earth System Models (impacting the climate contribution of shipping emissions). This study explores an approach to develop spatially explicit energy demand projections for shipping in alignment with the Shared-Socioeconomic Pathways framework and IAM projections of global fossil fuel demand. Our results show that shipping could require between 14.4EJ and 20EJ by 2050, corresponding to a 3% and 44% increase from 2018 for SSP1 and SSP3 scenarios. Furthermore, the energy projections we present in this publication can be combined with different fuel mixes to derive emission inventories for climate modeling and, thus, better understanding the challenges in mitigating emissions for shipping. Through that, we aim to present a framework to incorporate detailed spatial shipping inventories and increase transparency for the scientific community.
Towards Generalist Foundation Model for Radiology
Authors: Xie Weidi , Wu Chaoyi , Zhang Xiaoman , Zhang Ya , Wang Yanfeng
Published: September 2023

In this study, we aim to initiate the development of Radiology Foundation Model, termed as RadFM.We consider the construction of foundational models from the perspectives of dataset construction, model design, and thorough evaluation. Our contribution can be concluded as follows: (i), we construct a large-scale Medical Multi-modal Dataset, MedMD, which consists of 16M 2D and 3D medical scans with high-quality text descriptions or reports across various data formats, modalities, and tasks, covering over 5000 distinct diseases. To the best of our knowledge, this is the first large-scale, high-quality, medical visual-language dataset, with both 2D and 3D scans; (ii ), we propose an architecture that enables visually conditioned generative pre-training, i.e., allowing for integration of text input with 2D or 3D medical scans, and generate responses for diverse radiologic tasks. The model was initially pre-trained on MedMD and subsequently fine-tuned on the domain-specific dataset, which is a radiologic cleaned version of MedMD, containing 3M radiologic visual-language pairs, termed as RadMD; (iii), we propose a new evaluation benchmark, RadBench, that comprises five tasks, including modality recognition, disease diagnosis, visual question answering, report generation and rationale diagnosis, aiming to comprehensively assess the capability of foundation models in handling practical clinical problems. We conduct both automatic and human evaluation on RadBench, in both cases, RadFM significantly outperforms existing multi-modal foundation models. The codes, data, and model checkpoint will all be made publicly available to promote further research and development in the field.
Epigenetic analysis on RUNX3 mislocalization and its association with tumor microenvironment alteration in non-small cell lung cancer
Authors: Deng Yujie , Liu Weibin , Lin Mingqiang , Huang Chuanzhong , Shi Yi , Lu Jianping , Zheng Xinlong , Zhu Weifeng , Liu Tao , Zhang Longfeng , Chen Xiaohui
Published: September 2023

To investigate the epigenetic characteristics and tumor microenvironment status that might probably underlie RUNX3 mislocalization in NSCLC. Epigenetic markers, PD-L1 and CD3/8 were assessed in TMA and TCGA datasets. Methylation level at RUNX3 promoter was assessed via pyrosequencing. RNA-seq assays were performed. NSCLC patients’ survival with different patterns of RUNX3 expression was determined. Among NSCLC with RUNX3 negative, nuclear, cytoplastic and whole-cell expression, epigenetic markers differed significantly (all P<0.05). No difference on PD-L1 expression level or CD8/CD3 ratio existed in 4 or 2 RUNX3 distribution patterns (both P>0.05), as was verified in TCGA database. Pyrosequencing indicated higher methylation level on CpG1/2 would result in low RUNX3 expression, as was also associated with higher probability of local relapse (both P<0.05). RNA-Sq showed that the DEGs were primarily enriched in biological processes associated with tumor metastasis, like MMP11. Survival analysis demonstrated no survival discrepancy in various RUNX3 expression patterns of any stages (all P>0.05). No difference in TME markers were determined, either. Various RUNX3 expression patterns might in part result from different status of epigenetic alteration. RNA-Sq revealed that its expression might connect to genes associated with EMT or distant metastasis, and higher RUNX3 promoter methylation was correlated with local relapse.
Patern Formation and Diffusion-Driven Instability of a Modified Lengyel-Epstein Model
Authors: MEYVACI Müge , MERDAN Hüseyin , Yafia Radouane
Published: September 2023

We consider a modified Lengyel-Epstein model that is covered by a system of reaction diffusion equations and investigate the sufficient conditions on parameters of the model at which the diffusion driven instability occurs. We perform numerical simulations to support and extend the theoretical findings. Analytical results and numerical simulations show that the modified Lengyel-Epstein system with positive initial and non-flux boundary conditions presents Turing patterns under some conditions on its parameters.
Lengyel–Epstein system
2008 MSC: 35K57, 35K50, 92D25, 92C15 (2000 is the default)
Patients with secondary t-AML patients with TP53 Arg72Pro and SH2B3 W262R: A case report and review of the literature
Authors: Liu Yalu , Yue Ting
Published: September 2023

Background:
The incidence of therapy-related acute myeloblastic leukemia (t-AML) has increased in recent years owing to advances in tumor treatment. Compared to de novo AML, patients with t-AML have a poorer response to treatment and shorter overall survival. Genetic variants are associated with the risk of developing t-AML.
Case presentation: In this study, we report two cases of t-AML following treatment for diffuse large B-cell lymphoma. All the patients had previously undergone stem cell transplantation and high-dose chemotherapy. The time interval between the initial diagnosis and the onset of t-AML varied from 15 to 21 months. TP53 SNP rs1042522 and SH2B3 W262R polymorphisms were detected at baseline in both patients.
Conclusions: Based on the present findings and a review of the literature, we propose that patients with the GG and GC genotypes of the SNP rs1042522 and SH2B3 W262R polymorphisms may be at a higher risk of developing t-AML.
Apple Pomace as an Alternative Substrate for Butanol Production
Authors: Tigunova Olena , Bratishko Viacheslav , Shulga Sergiy
Published: September 2023

Butanol-producing strains Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 were used for research from "Collection of strains of microorganisms and plant lines for food and agricultural biotechnology" of the Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, glycerol (BASF, Germany) and apple pomace (total moisture 4%) after apple juice production. The aim of this work was to study the possibility of using apple pomace by domestic butanol-producing strains of Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 as a substrate. Producers were cultured on medium with different concentrations of apple pomace, glycerol was used for the inoculation. The presence of ethanol, acetone, and butanol in the culture liquid was determined using a gas chromatograph. It was determined that a significant part of the macrocomponent composition of the extracts can be used in bioconversion by producing strains of the genus Clostridium. It was determined that the highest concentration of butanol (10 g/dm3) was at a concentration of 120 g/dm3 in the extracts. The obtained data showed the possibility of using apple pomace as a substrate in biobutanol technology.
During a storm – the impact of the COVID-19 pandemic on the epidemiology of the most frequent cancers in Poland
Authors: Choręza Piotr , Owczarek Aleksander Jerzy , Kruk Wacław , Chudek Jerzy
Published: September 2023

Background
The late diagnosis, despite improving availability and accessibility of diagnostic procedures during the last decade in Poland and cooperation between specialist cancer centres, remains an unsolved problem. A new coronavirus SARS-CoV2 causing the COVID-19 disease occurred in the end of 2019. In a few months, it has become the most fundamental problem for public health worldwide. The accessibility to healthcare resources and diagnostic procedures has been drastically reduced because of the COVID-19 pandemic in 2019–2020. The study was aimed to assess the effect of the COVID-19 pandemic and slow cancer burden on the healthcare system of the most frequent cancers in Poland in the period 2015–2021.
Methods
Depersonalized, epidemiological data was obtained from the National Health Found of Poland. The epidemiological analysis was performed and divided into subregions, according to patients’ domicile. For each of the subregions, we have calculated the incidence rate per 100,000 standardized to the ESP 2013. The time points of providing the first healthcare service were considered as the time of cancer diagnosis.
Results
Colorectal cancer has become the leading oncological problem in Poland in recent years and the long-term trend shows a significant increase in the SIR in 2021 compared to 2020. The SIR of the lung cancer decreased in 2015–2019 across the country as well as the burden of kidney cancer has decreased in most of the subregions. The breast and prostate cancers were the most frequent cancer diagnosed in Poland among women and men respectively. Such a situation has remained stable till 2020 when the COVID-19 pandemic has occurred. In 2020, compared to 2019 a visible reduction of SIR of the breast cancer in most of the subregions was found, especially in the eastern areas. In 2020 when the COVID-19 pandemic occurred, a visible reduction of the SIR of C61 in most of the subregions was found.
Conclusions
Our results show a significant decrease in the SIR of the most frequent cancers diagnosed in Poland in 2020 compared to 2019. Subsequently, the excessive increase in 2021 was found, the most likely due to the gradual reduction of epidemic restrictions.
Study on the protein and peptide absorbents for capture and storage of CO2 : Interactions bwteen CO2 and amino acids
Authors: Long Si-Yu , Shen Nai-Kun , Liu Jin-Lei , Zhou Ling-Qiang , Tang Pei-Duo , Du Qi-Shi
Published: September 2023

Interactions between amino acid side chains and CO2 molecules are systematically evaluated using quantum chemical calculations and NMR experiments. The binding energies of CO2 in three proteins (phosphoenolpyruvate carboxykinase, rhodobacter capsulatus, and carbonic anhydrase II) are analyzed based on QM and MD simulations. In the 20 natural amino acids of proteins 13 charged or polar amino acids possess remarkable ability of electrostatic interaction with CO2 molecules. Aromatic amino acids (Phe, Tyr, and Trp) can form π–π stacking interactions with CO2 molecules that are comparable with common hydrogen bonds (ཞ20 kJ/mol). The best binding sites for CO2 molecules are the three alkaline amino acids (cations Arg+, Lys+, and His+). The acidic amino acids (anions Asp− and Glu−) could maintain a suitable pH condition that is necessary for CO2 releasing from proteins. Histidine may deliver CO2 molecules in proteins through the changes form His+ to His. The CO2 molecules also can form hydrophobic interaction with hydrophobic amino acids, and to be stored in hydrophobic pocket in proteincs. Two peptide absorbents are designed and synthesized, and measured by NMR technique. The study results may help to develop more powerful protain and peptide absorbents for CO2 capture and storage.
Primary myxoid pleomorphic liposarcoma in the anterior mediastinum a case report
Authors: Wen Rui , Tang Jinyan , Hu Xianwen , Shen Xiaotong , Li Chuan , Wang Pan
Published: September 2023

Liposarcoma is a relatively rare malignant tumour of the adipose tissue, can happen in any place, including myxoid pleomorphic liposarcoma (MPL) is a special subtype of liposarcoma, common in children and infants and young children, very low incidence of mediastinum. The literature on mediastinal myxoid pleomorphic liposarcoma published at home and abroad are only case reports, so the clinical symptoms and imaging features are lack of specificity, and the diagnosis is challenging. Finally, postoperative pathology is still needed to confirm the diagnosis. In order to further improve the understanding of myxoid pleomorphic liposarcoma, we report a case of primary mediastinal myxoid pleomorphic liposarcoma with chest pain as the main symptom. The patient was discharged after complete surgical resection. This paper aimed to the disease of clinical, radiological features, pathology, diagnosis and treatment were analyzed and summarized.
Prediction of Water Distribution Uniformity of Sprinkler Irrigation System Based on Machine Learning Algorithms
Authors: Elhussiny Khadiga T. , Hassan Ahmed M. , Habssa Ahmed Abu , Mokhtar Ali
Published: September 2023

The coefficients of uniformity (Christiansen's uniformity coefficient CU and distribution uniformity DU), an important parameter for designing irrigation systems, and is an accurate measure for water lose. That research include, three sprinklers were used to measure the CU and DU results based on operating pressure, heights of sprinkler, discharge, nozzle diameter, wind speed, humidity, highest temperature and lowest temperature to determine the best parameters for good irrigation design and also to select the best scenario effects on CU and DU values for square and triangular system based on four scenarios were applied to predict the DU based on machine learning algorithms. The main findings are 1) the highest CU was 86.7% in the square system of 2520 sprinkler under 200 kPa, height of 0.5m and the discharge was 0.855 m3/h (Nozzle 2.5mm). Furthermore, it was 87.3% in the triangled system under the same pressure and discharge however on the 1m height. 2) In CU, the highest of R2 is 0.796, 0.825 and 0.929 in RF, XGB and XGB-RF respectively in the first scenario. 3) On contrast, for the DU, the highest value of R2 is 0.7014, 0.4791 and 0.8275 in RF, XGB and XGB-RF respectively in the first scenario.
Transforming growth factor-β1 is associated with inflammatory resolution via promoting M2c macrophage polarization in lung injury mice
Authors: Jing Ren , Xie Xianlong , Liao Xiaoting , He Sheng , Mo Jianlan , Dai Huijun , Hu Zhaokun , Pan Linghui
Published: September 2023

Macrophage polarization is vital for the resolution of inflammation and tissue injury. Here, we investigated the role of transforming growth factor (TGF)-β1 on macrophages polarization. In the mouse model of ventilation-induced lung injury, high tidal volume-induced injury and inflammation were resolved 3 days post-ventilation (PV3d) to PV10d, with increased elastic fiber, proteoglycan, and collagen, as well as higher TGF-β1 level. M1 increased temporarily, whereas M2a and M2c began to increase from PV3d. In vivo upregulating or neutralizing TGF-β1 expression indicated that an appropriate expression of TGF-β1 is required to resolve lung injury and inflammation via regulating macrophage polarization. M1 are the main pro-inflammatory macrophages to induce pulmonary edema and inflammation. Inhibition of M1 to M2a is associated with pulmonary injury and inflammation, but M2b is contributed to the attenuation of serum interleukin (IL)-1β and pulmonary IL-6 levels. M2c contributed to the attenuation of serum and pulmonary IL-6 levels as well as serum tumor necrosis factor-α levels. TGF-β1 is a crucial factor to promote M2b and M2c polarization with the reduction of pulmonary IL-6 levels. Together, secretion of TGF-β1-mediated macrophage polarization plays a crucial role in the resolution of inflammatory lung injury.
Nurses’ views of patient- and family-centered care and its practices in peri-operative contexts in hospitals in Northern Ghana
Authors: Adugbire Bernard Atinyagrika , Jordan Portia Janine , Young Cornelle
Published: September 2023

Background
The purpose of the study was to explore the views of nurses on the concept of patient- and family-centered care (PFCC) and its practices in a peri-operative context in Northern Ghanaian hospitals
Methods
The study, using a qualitative explorative, descriptive and contextual research design was conducted at six selected hospitals located in the Northern part of Ghana. Purposive sampling was used to recruit participants for individual and focus group interviews. Data were generated between March and May 2022. Data saturation was reached with 27 participants comprising 15 individuals and 12 members from two focus group interviews. All interviews were digitally recorded and transcribed verbatim and the data analysed using thematic analysis.
Results
Two themes emerged from the study, namely nurses’ understanding of the concept of PFCC and how they see the benefits of PFCC in a peri-operative context. Subthemes of the nurses’ understanding of PFCC included their perceptions of PFCC, the need for collaboration, information sharing and communication and the importance of PFCC practices in a peri-operative context. Subthemes for benefits of PFCC in the peri-operative context included nurse-related benefits, patient- and family-related benefits and healthcare system-related benefits.
Conclusions
The study revealed that the concept PFCC is unfamiliar to the Ghanaian peri-operative context despite the positive perceptions exhibited by the nurses in terms of their understanding of PFCC and its related benefits to nurses, patients and families as well as the healthcare system.
Physical Properties of WR 90 (HD 156385) Based on Spectroscopic Study
Authors: Budi Bakuh Danang Setyo , Malasan Hakim Luthfi
Published: September 2023

The emission line on the spectrum of the Wolf-Rayet star shows an exceptional abundance of the elements helium, nitrogen, carbon, and oxygen w hen compared to the spectrum of other stars. In this work, observation of WR 90 (HD 156385) was conducted using a low-resolution spectrograph at Bosscha Observatory. Determination of stellar parameters has been implemented using two different methods: The first method derives estimation of photosphere temperature and radius using the Black Body continuum model approximation, second method derives estimation of stellar temperature, radius, and luminosity using PoWR (The Postdam Wolf-Rayet) spectrum grid model. Furthermore, with the addition of the asymptotic terminal velocity derived from the Gaussian decomposition of the optical spectrum emission line and the P-Cygni profile analysis of the high-resolution IUE UV spectrum, the mass loss rate of the Wolf-Rayet star has been determined. The data used in both methods are low- and highresolution ultraviolet spectra retrieved from the IUE (International Ultraviolet Explorer) archival and optical spectrum of our observations. The obtained stellar parameters appear to have small differences when compared with previous work.
Multivariate analysis of prognostic factors in patients with lip squamous cell carcinoma after surgery
Authors: Cheng Hao , Xu Jin-Hong , He Jia-Qi , Yang Xi-Yang , Shen Xu-Ning , Xu Xue-Lian
Published: September 2023

Background: Lip squamous cell carcinoma (LSCC) was one of the most common cancer types of head and neck tumors. This study aimed to reveal clinical factors affecting the prognosis of postoperative LSCC patients.
Methods: A total of 147 LSCC patients between June 2012 and June 2018 were collected and analyzed. There were 23 clinicopathological factors included in our study: age at diagnosis, gender, tumor location, pathological differentiation, American Joint Committee on Cancer (AJCC) stage, adjuvant chemotherapy, adjuvant radiotherapy, surgical margin, perineural invasion, extranodal extension (ENE), vascular invasion (VI), multiple primary tumors, age-adjusted Charlson comorbidity index (ACCI); body mass index (BMI), Glasgow prognostic Score (GPS), interquartile range (IQR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), prognostic nutrition index (PNI), eastern cooperative oncology group (ECOG) performance status (PS) score, systemic immune-inflammation index(SII), overall survival (OS), and progression-free survival (PFS). The univariate and multivariate Cox regression analyses were applied to reveal the independent prognostic factors in postoperative LSCC patients on PFS and OS. The benefit of adjuvant radiotherapy in various subgroups was also displayed by Kaplan-Meier curves.
Results: In postoperative LSCC patients, the 1-, 3- and 5-year PFS were 88.4%, 70.1%, and 57.8%, and the 1-, 3- and 5-year OS were 94.6%, 76.9%, and 69.4%, respectively. The analysis results indicated that age ≥ 70 years, later stage, surgical margin <5mm, higher PNI, and ACCI ≥5 indicate poorer PFS in postoperative LSCC patients (All P<0.05). Besides, postoperative LSCC patients with age ≥ 70 years, later stage, surgical margin < 5mm, higher GPS score, higher SII, and ACCI ≥5 tend to have a poorer OS (All P<0.05). Additionally, postoperative LSCC patients with ACCI < 5 and AJCC III-IV stage were more likely to benefit from adjuvant radiotherapy, but not for the other subgroups.
Conclusion: Our results showed that clinicopathological factors like age ≥ 70 years, close surgical margin, later stage, surgical margin < 5mm, higher PNI, higher GPS score, higher SII, and ACCI ≥5 had a great negative effect on the prognosis of postoperative LSCC patients. Postoperative LSCC patients with stage III-IV and ACCI < 5 can benefit from adjuvant radiotherapy.
The inherent violence of anti-Black racism and its effects on HIV care for Black sexually minoritized men
Authors: Quinn Katherine G , Walsh Jennifer L , DiFranceisco Wayne , Edwards Travonne , Takahashi Lois , Johnson Anthony , Dakin Andrea , Bouacha Nora , Voisin Dexter R
Published: September 2023

The goal of this study was to examine the effects of racial discrimination, depression, and Black LGBTQ community support on HIV care outcomes among a sample of Black sexually minoritized men living with HIV. We conducted a cross-sectional survey with 107 Black sexually minoritized men living with HIV in Chicago. A path model was used to test associations between racial discrimination, Black LGBTQ community support, depressive symptoms, and missed antiretroviral medication doses and HIV care appointments. Results of the path model showed that men who had experienced more racism had more depressive symptoms and subsequently, missed more doses of HIV antiretroviral medication and had missed more HIV care appointments. Greater Black LGBTQ community support was associated with fewer missed HIV care appointments in the past year. This research shows that anti-Black racism may be a pervasive and harmful determinant of HIV inequities and a critical driver of racial disparities in ART adherence and HIV care engagement experienced by Black SMM. Black LGBTQ community support may buffer against the effects of racial discrimination on HIV care outcomes by providing safe, inclusive, supportive spaces for Black SMM.
Clinical and Economic Outcomes of a Pharmacogenomics-enriched Comprehensive Medication Management Program in a Self-insured Employee Population
Authors: Fragala Maren , Keogh Murray , Goldberg Steven , Lorenz Raymond , Shaman Jeffrey
Published: September 2023

Clinical and economic outcomes from a pharmacogenomics-enriched comprehensive medication management program were evaluated over 26-months in a self-insured employee population (n=452 participants; n=1,500 controls) using propensity matched pre-post design with adjusted negative binomial and linear regression models. After adjusting for baseline covariates, program participation was associated with 39% fewer inpatient (p=0.05) and 39% fewer emergency department (p=0.002) visits, and with 21% more outpatient visits (p<0.001) in the follow-up period compared to the control group. Results show pharmacogenomics-enriched comprehensive medication management can favorably impact healthcare utilization in a self-insured employer population by reducing emergency department and inpatient visits and can offer potential for cost savings. Self-insured employers may consider implementing pharmacogenomics-enriched comprehensive medication management to improve the healthcare of their employees.
Bright quantum photon sources from a topological Floquet resonance
Authors: Barzanjeh Shabir , Afzal Shirin , Zimmerling Tyler , Rizvandi Mahdi , Taghavi Majid , Hrushevskyi Taras , Kaur Manpreet , Van Vien
Published: September 2023

Entanglement, a fundamental concept in quantum mechanics, plays a crucial role as a valuable resource in quantum technologies. The practical implementation of entangled photon sources encounters obstacles arising from imperfections and defects inherent in physical systems and microchips, resulting in a loss or degradation of entanglement. The topological photonic insulators, however, have emerged as promising candidates, demonstrating an exceptional capability to resist defect-induced scattering, thus enabling the development of robust entangled sources.
Despite their inherent advantages, building bright and programmable topologically protected entangled sources remains challenging due to intricate device designs and weak material nonlinearity. Here we present an advancement in entanglement generation achieved through a non-magnetic and tunable resonance-based anomalous Floquet insulator, utilizing an optical spontaneous four-wave mixing process. Our experiment demonstrates a substantial enhancement in entangled photon pair generation compared to devices reliant solely on topological edge states and outperforming trivial photonic devices in spectral resilience. This work marks a step forward in the pursuit of defect-robust and bright entangled sources that can open avenues for the exploration of cascaded quantum devices and the engineering of quantum states. Our result could lead to the development of resilient quantum sources with potential applications in quantum technologies.
Linear responses of soil microbiomes, metagenomic and metabolomic functioning across ecosystems along water gradients in the Altai region, northwestern China
Authors: Fu Qi , Qiu Yingbo , Zhao Jiayi , Li Jiaxin , Xie Siqi , Liao Qiuchang , Fu Xianheng , Huang Yu , Yao Zhiyuan , Dai Zhongmin , Qiu Yunpeng , Li Furong , Chen Huaihai
Published: September 2023

Background
To investigate microbial communities and their contributions to carbon and nutrient cycling along water gradients can enhance our comprehension of climate change impacts on ecosystem services.
Results
We conducted an assessment of microbial communities, metagenomic functions, and metabolomic profiles within four ecosystems, i.e., desert grassland (DG), shrub-steppe (SS), forest (FO) and marsh (MA) in the Altai region of Xinjiang, China. Soil total carbon (TC), total nitrogen, NH4+, and NO3- increased linearly, but pH decreased with soil water gradients. Microbial abundances and richness also increased with soil moisture except the abundances of fungi and protists being lowest in MA. Within prokaryotes, the relative abundances of Proteobacteria and Acidobacteria increased, whereas those of Actinobacteria and Thaumarchaeota decreased along water gradients. In fungi and protists, Basidiomycota and Mortierellomycota, Evosea and Endomyxa became dominant in FO and MA, respectively, but the relative abundance of Cercozoa decreased along soil moisture gradients. The β-diversity of microbiomes, metagenomic and metabolomic functioning were linearly distributed along soil moisture gradients, significantly associated with soil factors of TC, NH4+, and pH. For soil metagenomic functions, the metabolic genes related to Carbohydrate (CO2 fixation, Di- and oligosaccharides, Fermentation, and One-carbon metabolism), Iron (Iron acquisition in Vibrio and Campylobacter iron metabolism) decreased with soil moisture, while genes related to the metabolisms of Nitrogen (Ammonia assimilation, Denitrification, Nitrogen fixation, and Nitrosative stress) and Potassium (Potassium homeostasis) increased linearly along water gradients. Additionally, MA harbored the most abundant metabolomics dominated by lipids and lipid-like molecules (Erucic acid, Hypogeic acid, and Kojibiose, etc.), and organic oxygen compounds (Maltotetraose, Quinone, Sucrose, and Trehalose, etc.), except certain metabolites showing decline trends along water gradients, such as N'-Hydroxymethylnorcotinine and 5-Hydroxyenterolactone.
Conclusions
Our study suggests that future ecosystem succession facilitated by changes in rainfall patterns will significantly alter soil microbial taxa, functional potential and metabolite fractions.
Genome-wide Identification of the CIPK Gene Family in Jasmine and Expression Analysis Under Salt Stress
Authors: Chen Kai , Cao Tingting , Yin Lili , Huang Xin , Zhang Xuyao , Wu Ruigang
Published: September 2023

Background
Various CIPK genes are involved in abiotic stress responses in plants. Despite the economic importance of jasmine (Jasminum sambac), and the availability of genome data, there are few reports analyzing the CIPK gene family. The aim of this study was to perform a phylogenetic analysis and characterization of the CIPK genes in jasmine.
Results
In the present study, A total of 17 CIPKs were identified, which were unevenly distributed on chromosomes. The JsCIPK protein sequences contained 311–781 amino acids, with a molecular weight of 35.05–87.58 kDa. Phylogenetic analysis revealed that the 17 JsCIPKs could be divided into five classical branches. JsCIPK genes with higher homology showed greater similarity between conserved protein motifs. Collinearity analysis demonstrated that 13 gene pairs in Arabidopsis were collinear with the jasmine sequences. Various hormone-related response- and stress-induced elements were observed in the promoter region of JsCIPK genes. Furthermore, the expression of JsCIPK genes varied in different organs. Finally, the expression analyses of eight JsCIPKs under salt stress were performed.
Conclusions
A systematic analysis of the CIPK gene family in Jasmine and expression analysis under salt stress was provided. These findings lay a foundation for future functional studies of JsCIPKs in jasmine related to growth and development and responses to abiotic stress.
Seroprevalence of Contagious Bovine Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) in Cattle from Karamoja Region, North-eastern Uganda
Authors: Tweyongyere Robert , Nkamwesiga Joseph , Etiang Patrick , Mugezi Israel , Wamala Henry , Wasswa Auther Tamale , Kamusiime Maureen , Ainebyoona Solomon , Abizera Harriet , Mwiine Frank Norbert , Muhanguzi Dennis
Published: September 2023

Background
Contagious bovine pleuropneumonia [CBPP] is a transboundary animal disease of cattle caused by Mycoplasma mycoides subsp. mycoides biotype small colony [MmmSC]. CBPP causes severe economic losses to livestock producers in sub-Saharan Africa mainly due to high mortality, morbidity, reduction in productivity as well as livestock trade restrictions. This study aimed at determining seroprevalence of MmmSC in cattle from Karamoja region, north-eastern Uganda; data that are required to design and implement risk based CBPP control program.
Methods
We randomly collected blood samples from 2,300 cattle spread across Karamoja region. Serum was extracted and screened for antibodies against Mycoplasma mycoides subsp. mycoides biotype small colony [MmmSC] using the competitive enzyme linked immunosorbent assay [cELISA]
Results
A quarter [25.4%; 95% CI: 23.7–27.3] of the screened cattle [n = 2,300] were sero-positive for MmmSC. Amudat and Kaabong districts recorded the lowest [12.3%] and highest [30.7%] MmmSC seroprevalence respectively. Increasing age, overnight stay in cattle kraals and location [certain districts, villages, herds and sub counties] of the cattle herds, the factors that promote animal commingling, were the most significant risk factors of seroconversion with MmmSC.
Conclusion
Results from this study indicated a higher seroprevalence of MmmSC in Karamoja region cattle herds than previously reported implying that CBPP endemicity has increased over time. To be effective, CBPP vaccination programs should target high risk herds along the international borders and other hotspot areas [e.g., parishes or sub counties]. Additionally, animals should be vaccinated against CBPP prior to being considered for overnight stays in protected kraals.
Evaluation of colorectal cancer in ulcerative colitis reveals key immune factors during its malignant transformation
Authors: Ma Jiao , Wang Qing , Wang Chaoye , Liu Chuwei , Yu Yiling , Zhao Qi , Ren Jian , He Weiling
Published: September 2023

BACKGROUND & AIMS:
Ulcerative colitis (UC) is linked to an increased risk of colitis-associated colorectal cancer (CAC), which accounts for approximately 15% of UC-related deaths. Despite this significant impact on patients, the mechanism behind how UC promotes cancer development remains unknown. The present study aims to investigate alterations in the immune microenvironment during the malignant transformation of UC, shedding light on the underlying mechanisms of UC carcinogenesis.
METHODS
We collected single-cell transcriptome samples of 41 healthy samples, 45 UC samples, and 148 colorectal cancer(CRC) samples from public databases. Using the UC-CRC signature, we were able to screen for CAC-like samples. Based on those datasets, several bioinformatics analyses were performed on 228,538 immune cells to evaluate the immune microenvironment from UC to CAC.
RESULTS
Using predefined UC-CRC signature, we screened 14 CAC-like samples and revealed an immune remodeling process from healthy tissue to UC and CAC-like samples, particularly involving the VEGFA_Macro cells and Treg cells. VEGFA_Macro cells was significantly enriched in UC and CAC-like samples, showed a phenotype alteration during disease progression, and expressed more inflammation-related genes and signal pathways. Additionally, the proportion of Treg cells gradually increased with disease progression, potentially promoting an immunosuppressive microenvironment. Comparative analysis of the immune microenvironment between CAC-like and sporadic CRC(sCRC) samples revealed higher levels of myeloid cells but reduced CD8 + T cells in CAC-like samples. Finally, we simplified the UC-CRC signature for ease of clinical use in screening CAC-like samples.
CONCLUSIONS
Our results may help improve the understanding dynamic change of immune microenvironment from UC to CAC and provide clues for further exploration of strategies to prevent carcinogenesis of UC.
Enhancing Corrosion Resistance of Anodic Oxide Films through Zirconium and Titanium Salts Composite Sealing Process: An Optimization and Morphological Study
Authors: Yang Kaixuan , Yang Fan , Huang Yijia , Yu Yang , Xu Wenyuan , Hu Lin , Liu Jianping
Published: September 2023

In this paper, zirconium and titanium are used as the main components of a sealing agent to seal composite holes. The effects of zirconium content, titanium content, pH, time, and temperature on the sealing effect of the oxide film in the environmentally friendly zirconium salt and titanium salt composite sealing process are studied by the orthogonal experiment and response surface method. The optimization results show that the best sealing conditions of the composite salt are: ammonium fluotitanate 6.0 g/L, ammonium fluozirconate 6.0 g/L, pH 4.0, and sealing time 20 min. The sealing temperature is 35°C. After analyzing the micro-morphology and surface elements of the oxide film sealed by composite salt, the authors found that the porous structure of the oxide film surface obviously disappeared after sealing, and the oxide film surface after sealing was mainly composed of Zr, Ti, O, Al, and other elements.
Highly expressed RPLP2 inhibts ferroptosis to promote hepatocellular carcinoma progression and predicts poor prognosis
Authors: Guo Jiaxing , Huang Meiyuan , Deng Shuang , Wang Haiyan , Wang Zuli , Yan Bokang
Published: September 2023

Background
RPLP2, an integral part of ribosomal stalk, plays an important role in the tumorigenesis of various cancers. However, its specific effect on HCC remains elusive.
Methods
TCGA, GTEx, HCCDB, HPA, UALCAN, MethSurv, TISIDB, K-M plotter, FerrDb, RNAactDrug, STRING, Cytoscape and R studio were conducted for bioinformatics analysis. RPLP2 expression level in HCC was verified by IHC and western blot. IHC was used to demonstrate the immune cell infiltration. Functional experiments including CCK8, transwell and colony formation assays, and nude mice xenograft model were performed for in vitro and in vivo validation. Western blot, IHC, CCK8 assay and detection of GSH and lipid ROS were adopted to determine the effect of RPLP2 on the ferroptosis of HCC cells.
Results
Here, we demonstrated that elevated level of RPLP2 is strongly associated with advanced clinicopathologic features, and predicts poor prognosis of HCC patients. Additionally, DNA methylation level of RPLP2 decreases in HCC, and significantly correlated with patients outcome. Moreover, high RPLP2 expression level is linked closely to the unfavorable immune infiltration. Most importantly, RPLP2 positively associates with ferroptosis suppressor GPX4, and inhibition of RPLP2 could lead to the acceleration of ferroptosis to suppress tumor progression of HCC. Last, drug sensitivity analysis predicts many drugs that potentially target RPLP2.
Conclusion
Together, our study reveals previous unrecognized role of RPLP2 in HCC, and provides new regulatory mechanism of ferroptosis, indicating RPLP2 may be a novel therapeutic target for HCC.
Object Recognition and Detection Using Convolutional Neural Network in Deep Learning
Authors: Shahalinejad Sahand , Nooshyar Mehdi , Fakheri Mir Majid , Imani Nima
Published: September 2023

Object recognition technology in the field of computer vision for finding and identifying objects in an image or video sequence. Humans recognize a multitude of objects in images with little effort, although the image of the objects may vary somewhat in different viewpoints, in many different sizes and scales or even when they are translated or rotated. Objects can even be recognized when they are partially obstructed from view. This task is still a challenge for computer vision systems. Many approaches to the task have been implemented over multiple decades In many computer vision systems, object detection is the first task being performed as it allows us to obtain further information regarding the detected object and about the scene. In recent years, the use of deep learning has attracted the attention of researchers, Deep learning uses multiple layers to extract raw features from high-level features the machine divides each complex concept into simpler concepts. The proposed method is based on AlexNet architecture. In this method, a convolutional neural network-based architecture with a small amount of data can detect objects in the image, the data used is the March database, so this process identifies thirty pictures in four separate classes with 100% accuracy.
Development of a health promoting sports clubs intervention: an intervention mapping process analysis
Authors: Hoye Aurélie Van , Johnson Stacey , Rostan Florence , Lemonnier Fabienne , Tezier Benjamin , Geidne Susanna , Donaldson Alex , Vuillemin Anne
Published: September 2023

The development of health promotion interventions using settings-based approaches in sports clubs remains a challenge. Intervention implementation in real world settings should be specific to a sports club’s context, especially considering the voluntary nature of the administration of most sporting organizations. The present paper aims to map and analyze the development of the PROSCeSS intervention, designed to support sports clubs to implement health promotion interventions. A case study design was used, mobilising the 5-steps of Intervention Mapping — needs assessment, matrix of change, theory of action, program design, plan for adoption and implementation and evaluation — to describe the multi-phase intervention and discuss its adaptation. Results encompass the main findings of a literature review and a concept mapping study as a needs assessment, a Delphi study and health promoting sports club intervention framework to design the matrix of change, a case study among sports clubs and sports federations to select the theory of action, the intervention co-construction to create the program, results from a pilot study to validate the plan for adoption and implementation and presentation of the e-PROSCeSS questionnaire creation and hybrid type-3 protocol as an evaluation method. The discussion explores the tensions created between evidence driven strategies and the needs and habits of sports club stakeholders, the use of top-down versus bottom-up approaches in multi-level design, and reflections on the recursive rather than linear process adopted by the authors. Future research should concentrate on intervention design description, as well as the importance of building iterative feedback when designing interventions.
Construction and validation of a neutrophil extracellular traps- related genes prognostic model in lung adenocarcinoma
Authors: Yang Wenxing , Deng Xinyue , Yin Haoyuan , Li Ying , Ji Yuxin , Lv Xiangyang , Ye Lianhua
Published: September 2023

Purpose The specific regulatory mechanism of neutrophil extracellular traps (NETs) in lung adenocarcinoma (LUAD) is unclear.
Methods LUAD related datasets were obtained via the cancer genome atlas (TCGA) and gene expression omnibus (GEO) databases. Differentially expressed genes (DEGs) between LUAD and control samples were sifted out, and the critical modules were yielded via weighted gene co-expression network analysis (WGCNA). NETs score of TCGA-LUAD set was estimated based on 136 NETs related genes. Further, DEGs1 between high/low scoring groups were interscted with the DEGs, critical module genes to acquire risk model genes via univariate Cox and least absolute shrinkage and selection operator (LASSO) analyses.
Results The six risk model genes were acquired, and the expression trends of which were all consistent in public datasets and clinical tissues. The survival percentages of LUAD patients increased with increasing risk score. Besides, the gene-drug network and the miRNA-mRNA-TF network targeting model genes were genereted, including ABCC2-Tamoxifen and hsa-mir-8485-IGF2BP1-ELK1.
Conclusion Through bioinformatic analysis, the study constructed a prognostic risk model according to IGF2BP1, ABCC2, BCL2L10, FIGF, EMR3, and WNT7A in LUAD, providing new ideas for related studies of LUAD.