bluuebunny/crossref_metadata_2025 huggingface dataset:
Study the Phenotypic and Antimicrobial Susceptibility of Pseudomonas aeruginosa Isolated Clinically from Baghdad Hospitals
Authors: Ahmad Ruaa , Mohammed Rana , Alagely Huda
Published: March 2024

Pseudomonas aeruginosa, is negative to gram stain, take the rod shape and considered as strictly aerobic. the most affect bacteria in nosocomial infection. P.aeruginosa is an opportunistic pathogen bacterium that can made serious infections in human who are immune compromised, such as urinary tract infection, skin  and ear infection, and others. One hundred twenty one sample were taken from patients in different sites of infections (ear, wound, UTI , burn) from AL-Yarmouk, Ghazi Al-Hariri hospitals in Baghdad during the period from November 2016 to February 2017. Fifty seven isolates were diagnoses as P. aeruginosa by characteristic in culture media, tests and API 20E. forty  isolates involved in our study ten from each source. Antibiotic susceptibility test performed for forty clinically isolates of P. aeruginosa by the disk diffusion method against some antibiotics belong to different group and the results revealed that bacteria is multi-drug resistance (MRD) as well, revealed the most compound that have activity against Pseudomonas. aeruginosa were Imipenem, Pipracillin, and Ceftazidime. Biofilms are currently estimated and the P. aeruginosa reflect high ability to produce biofilm. All isolates used in this study formed biofilm with differences in thickness of formed layer. In this study, we concluded  P. aeruginosa is one of the most common gram-negative bacteria involved in hospital infections causing opportunistic infection because they have intrinsically and acquired resistant to a number of antimicrobial agents and produces a number of exoproducts which are implicated in the pathogenesis of   P. aeruginosa infections.
Parahisian Stimulation Through Unusual Vascular Access - Case Report
Authors: Mendes Carlos Robério , Albino Paulo
Published: March 2024

Introduction: To report the case of a patient with a total atrioventricular block with significant ventricular dysfunction whose pacemaker implantation and physiological stimulation occurred through the iliac vein due to tortuosities in other vessels that prevented the procedure. Method: Information was obtained by reviewing the medical records, interviewing the patient, photographing the diagnostic methods to which the patient was submitted, and reviewing the literature. Conclusion: The reported case and published publications bring to light the discussion of pacemaker implantation sites and physiological stimulation in a complex situation that, although it occurs in a minority of cases, when well it is capable of obtaining satisfactory results.
Contenido multimedia generado por el estudiante como estrategia de aprendizaje activo y colaborativo: Una experiencia piloto en la ETSIT de la Universidad de Málaga
Authors: Boluda Ruiz Rubén , del Castillo Vázquez Beatriz , García Zambrana Antonio
Published: March 2024

Este libro surge para dar respuesta a la urgente necesidad de incrementar los niveles de motivación y de participación de los estudiantes en el ámbito universitario, concretamente en algunos de los Grados que oferta la Escuela Técnica Superior de Ingeniería de Telecomunicación (ETSIT) de la Universidad de Málaga relacionados con la Ingeniería de Telecomunicación. La disminución de los niveles de motivación y participación de los estudiantes, tal como se viene observando desde hace unos años, se ha acentuado aún más desde el período de enseñanza virtual debido a la emergencia de la COVID-19. Ante este desafío, se propone como solución innovadora la creación e implementación de una nueva estrategia de aprendizaje activo y colaborativo que se basa en la generación de contenido multimedia por parte de los estudiantes, buscando siempre un aprendizaje de mayor calidad, especialmente en asignaturas obligatorias de Grado. Al mismo tiempo, los estudiantes sin duda pueden desarrollar y fortalecer habilidades transversales como el trabajo en equipo, la comunicación oral y el pensamiento crítico y creativo, todas esenciales en una potencial carrera profesional. En el experimento participaron de manera voluntaria estudiantes de diversas titulaciones y grupos de edad. Los resultados del experimento revelan un alto nivel de satisfacción por parte de los estudiantes con esta innovadora estrategia de aprendizaje y un alto éxito en el logro del objetivo principal, concluyendo que el grado de madurez y responsabilidad académica de los estudiantes involucrados presenta un impacto significativo. Se espera que este libro no solo sea una fuente de información valiosa, sino también una inspiración para todos aquellos comprometidos con la mejora de la educación universitaria. Que su lectura conduzca a nuevas ideas, reflexiones y acciones que contribuyan a un futuro más prometedor para la enseñanza y el aprendizaje en nuestro entorno académico.
Treatment of canine perineal hernia with a fascia lata graft is noninferior to the elevation of the internal obturator muscle: a prospective randomized trial of 66 dogs
Authors: Åhlberg Tuuli M. , Salla Kati , Laitinen-Vapaavuori Outi M. , Mölsä Sari H.
Published: March 2024

OBJECTIVE

To compare the recurrence rate after herniorrhaphy of canine perineal hernia (PH) using elevation of the internal obturator muscle (EIOM) or fascia lata graft (FLG) and assess how clinical signs related to defecation evolve during 12 months postoperatively.

ANIMALS

66 client-owned male dogs undergoing PH surgery between March 1, 2017, and December 31, 2020.

METHODS

Dogs were randomized into 2 groups (EIOM = 36; FLG = 30) applying 2 stratification factors: preoperative bladder location and the defecation signs score (DSS). Follow-up visits were at 2 weeks and 3, 6, and 12 months postoperatively. The main outcome, the recurrence of PH was evaluated by rectal examination. We evaluated the noninferiority of FLG to EIOM from the difference in recurrence proportions between the techniques (95% CI), comparing the CI with a pre-defined noninferiority margin (15%). To evaluate defecation signs, the DSS was calculated from the owner questionnaire and assessed with a linear mixed model (P < .05 significant).

RESULTS

In 63 dogs attending the 12-month follow-up, the recurrence rate was 8.8% (3/34) in the EIOM and 10.3% (3/29) in the FLG group. The CI (–11.94% to 14.99%) was below the pre-defined margin, indicating the noninferiority of FLG. After surgery, the DSS decreased (P < .001), remaining low during the follow-up.

CLINICAL RELEVANCE

FLG was non-inferior to EIOM when considering recurrence. The DSS decreased postoperatively and complications were uncommon. FLG is a useful alternative for the treatment of canine PH.
Oxidative Stress and Thrombophilia: Focus on Pregnancy-Related Pathophysiological Mechanisms
Authors: Dimitrijevic A , Jakovljevic V , Jovic N , Djuric J , Andric K , Jovic J Joksimovic
Published: March 2024

Thrombophilia, characterized by an increased tendency to form blood clots, presents a substantial risk during pregnancy, potentially impacting maternal and fetal outcomes. This review explores the intricate relationship between thrombophilia and oxidative stress, shedding light on their combined influence on blood clot formation. Specifically, endothelial dysfunction, driven by oxidative stress, emerges as a pivotal factor in thrombophilia, setting the stage for increased platelet activation and altered coagulation factors. Factors like Factor V Leiden mutation, Prothrombin G20210A mutation, and deficiencies in antithrombin, protein C, and protein S contribute to the pro-thrombotic state observed in thrombophilia. Furthermore, inflammation, closely intertwined with oxidative stress, exacerbates the risk of blood clot formation. Inflammatory responses lead to endothelial activation, altered endothelial function, and increased adhesion molecules expression, disrupting the delicate balance between pro- and anti-coagulant factors. Chronic inflammatory conditions, such as autoimmune disorders, potentiate a persistent state of heightened clotting risk. Additionally, impaired antioxidant defenses compound the prothrombotic state by allowing the accumulation of reactive oxygen species, thereby contributing to oxidative stress-induced endothelial dysfunction. Understanding the interplay between these factors is crucial for tailored thrombophilia management, particularly in pregnancy. Treatment strategies encompass a multifaceted approach, including anticoagulant medications, lifestyle modifications, and targeted interventions to improve endothelial health. The complex nature of thrombophilia underscores the need for a collaborative healthcare approach, involving hematologists and high-risk pregnancy specialists. Early detection, vigilant monitoring, and timely intervention are paramount in mitigating risks for both mother and baby.
Variance and information potential of some random variables
Authors: Motronea Gabriela , Pepenar Alin
Published: March 2024

We investigate random variables for which the variance and the information potential satisfy a preservation law.
Explainable AI for binary and multi-class classification of leukemia using a modified transfer learning ensemble model
Authors: Deshpande Nilkanth Mukund , Gite Shilpa , Pradhan Biswajeet
Published: March 2024

In leukemia diagnosis, automating the process of decision-making can reduce the impact of individual pathologists' expertise. While deep learning models have demonstrated promise in disease diagnosis, combining them can yield superior results. This research introduces an ensemble model that merges two pre-trained deep learning models, namely, VGG-16 and Inception, using transfer learning. It aims to accurately classify leukemia subtypes using real and standard dataset images, focusing on interpretability. Therefore, the use of Local Interpretable Model-Agnostic Explanations (LIME) is employed to achieve interpretability. The ensemble model achieves an accuracy of 83.33% in binary classification, outperforming individual models. In multi-class classification, VGG-16 and Inception reach accuracies of 83.335% and 93.33%, respectively, while the ensemble model reaches an accuracy of 100%.
The therapeutic effect of ultrasound targeted destruction of schisandrin A contrast microbubbles on liver cancer and its mechanism
Authors: Wang Xiaohui , Wang Feng , Dong Pengfei , Zhou Lin
Published: March 2024

Background

The aim of the study was to explore the therapeutic effect of ultrasound targeted destruction of schisandrin A contrast microbubbles on liver cancer and its related mechanism.

Materials and methods

The Span-PEG microbubbles loaded with schisandrin A were prepared using Span60, NaCl, PEG-1500, and schisandrin A. The loading rate of schisandrin A in Span-PEG composite microbubbles was determined by ultraviolet spectrophotometry method. The Walker-256 cell survival rate of schisandrin A was determined by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay. The content of schisandrin A in the cells was determined by high performance liquid chromatography. Ultrasound imaging was used to evaluate the therapeutic effect in situ. Enzyme linked immunosorbent assay (ELISA) was used to measure the content of inflammatory factors in serum. Hematoxylin-eosin (HE) staining was used to observe the pathological changes of experimental animals in each group. Immunohistochemistry was used to detect the expression of hypoxia inducible factor-1α (HIF-1α), vascular endothlial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (VEGFR-2) in tumor tissues, and western blot was used to detect the protein expression of phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway in tumor tissues.

Results

The composite microbubbles were uniform in size, and the particle size distribution was unimodal and stable, which met the requirements of ultrasound contrast agents. The loading rate of schisandrin A in Span-PEG microbubbles was 8.84 ± 0.14%, the encapsulation efficiency was 82.24±1.21%. The IC50 value of schisandrin A was 2.87 μg/mL. The drug + microbubbles + ultrasound (D+M+U) group had the most obvious inhibitory effect on Walker-256 cancer cells, the highest intracellular drug concentration, the largest reduction in tumor volume, the most obvious reduction in serum inflammatory factors, and the most obvious improvement in pathological results. The results of immunohistochemistry showed that HIF-1α, VEGF and VEGFR-2 protein decreased most significantly in D+M+U group (P < 0.01). WB results showed that D+M+U group inhibited the PI3K/AKT/mTOR signaling pathway most significantly (P < 0.01).

Conclusions

Schisandrin A had an anti-tumor effect, and its mechanism might be related to the inhibition of the PI3K/AKT/mTOR signaling pathway. The schisandrin A microbubbles could promote the intake of schisandrin A in tumor cells after being destroyed at the site of tumor under ultrasound irradiation, thus playing the best anti-tumor effect.
Reshaping Prognosis: Atrial fibrillation ablation in a case of severe tachycardiomyopathy in a young patient
Authors: Matei Diana-Cristina , Nistor Zamfira-Claudia , Burcin Alexandru Paul , Dragotoiu Nic Claudiu , Mandeş Leonard Alexandru , Antohi Elena Laura , Sipos Sergiu-Nicolae
Published: March 2024

Tachycardiomyopathy, characterized by ventricular systolic dysfunction resulting from persistent tachycardia, poses a significant challenge in cardiovascular disease management. This article presents the case of a 31-year-old male patient with dilated cardiomyopathy and recurrent persistent atrial fibrillation, admitted due to heart failure decompensation. Ischemic and inflammatory etiologies for heart failure were ruled out in prior assessments. Despite multiple antiarrhythmic drugs and attempted electrical cardioversion, sinus rhythm was not maintained. Echocardiography confirmed a decline in the already reduced ejection fraction. Atrial fibrillation cryoablation was performed, and procedural specifics are detailed. Subsequent follow-up visits revealed a noteworthy improvement in exercise tolerance and ventricular function. This case underscores the importance of considering tachycardiomyopathy in the differential diagnosis of new-onset or worsening heart failure and highlights the potential for symptom resolution and ventricular function recovery with atrial fibrillation ablation.
Potential of Dimethyl Sulfoxide Modified Kaolin and Cetyl Trimethylammonium Bromide as Amoxycillin Adsorben
Authors:   Program Study of Pharmacy, Faculty of Pharmacy, Universitas Bakti Tunas Husada, Cilolohan Street No. 36, Tasikmalaya City, West Java, Indonesia, Wardani Gatut Ari , Damayanti Ratih , Fathurohman Mochamad , Hidayat Taufik , Nofiyanti Estin
Published: March 2024

The large use of amoxicillin allows this antibiotic to enter the environment in large quantities and cause pollution. The adsorption technique can be used as a method to remove amoxicillin contaminants in wastewater by utilizing kaolin as an adsorbent. This study aims to determine the characteristics of kaolin as an adsorbent and determine the ability of kaolin to adsorb amoxicillin. Kaolin was activated with hydrochloric acid and modified using Dimethyl sulfoxide (DMSO) and cetyl trimethylammonium bromide (CTAB). The results showed that the characteristics of DMSO and CTAB-modified kaolin showed a new peak which was an O-H bending vibration in functional group analysis using FTIR. X-ray diffraction results show that the distance between planes in the kaolin structure is larger. Meanwhile, the results of the SEM analysis showed that the surface morphology of kaolin had a higher level of crystallinity than before which proved an increase in the adsorption capacity of kaolin. The adsorption kinetics follows the Santosa kinetic equation model with an adsorption rate of 0.004 min-1 and an equilibrium constant value of 0.007 L.mol-1 . The adsorption isotherm test follows the Freundlich isotherm equation model with an adsorption constant value of 561.694 L.mg-1 and an empirical constant value of 0.270.
Usability Study of the Mommy-Be App: Exploring the Experience of Breastfeeding Mothers in Eastern Indonesia
Authors: Syam Azniah , Dewi Indra , Firawati Firawati
Published: March 2024

Objective: The rise of mobile health (mHealth) apps has brought attention to their potential in supporting breastfeeding mothers and preventing postpartum depression (PPD). This study aims to evaluate the usability of Mommy-Be, a mobile phone app designed to support breastfeeding women and help them to acknowledge of their mental health and breastfeeding efficacy during lactation period.
 
Theoretical Framework: This study uses Usability theory to analyze the use of mHealth applications that proposed by Nielsen model.
 
Method: This study combines quantitative and qualitative methods, that asses Mommy-Be app's usability among breastfeeding mothers using the Mobile Application Usability Questionnaire (MAUQ) and conducted in-depth interviews with a subset of participants. The study included 50 breastfeeding mothers in Makassar, Indonesia, who were primarily between the ages of 19 and 42 and had diverse educational and employment backgrounds. Four weeks after using the Mommy-Be app, revealed a generally positive impression of the application.
 
Result: Based on MAUQ three dimensions, ease of use, interface satisfaction, usefulness, this study has been determined that the utilization of a Mommy-Be application is both acceptable and feasible as a mobile health (mHealth) solution for breastfeeding mothers. The mothers experience revealed diverse user impressions of the Mommy-Be application. Participants generally found it easy to use, with a favorable interface preference. Participants expressed various benefits of using Mommy-Be, including increased awareness of breastfeeding activities, assistance in meeting nutritional needs, and early detection of postpartum depressive disorders.
 
Recommendations: To enhance the application's utility, participants suggested improvements, such as data export capabilities, better module integration, consideration of the target audience's resources regarding internet access and the inclusion of educational content, such as the provision of interconnection with multiple lactation counsellors.
The Role of Forensic Accounting to Reduce Financial Corruption Practices and Improving the Quality of Financial Reports
Authors: Bakhit Mashael Abdelrahim Abdelrahman
Published: March 2024

Purpose: The study aims to identify the role of forensic accounting in reducing financial corruption practices and improving the quality of financial reports by applying it to Saudi joint stock companies.
 
Methodology: the study used descriptive approach, conducted a questionnaire to collect data and distributed it to the study’s population consisting of shareholders, investors, and workers in joint-stock companies in the Kingdom of Saudi Arabia.
 
Results and conclusion: The most important results of the study were that forensic accounting played a major role in combating financial corruption and business sustainability, and that academic and practical qualifications have a very important impact in discovering financial corruption, and the effectiveness of forensic accounting also led to improving the quality of financial reports. The researcher also recommends the necessity of adopting the subject of forensic accounting within the educational curriculum as a basic subject, as a preliminary step toward advancing the profession of forensic accounting, cooperating with educational institutions and investing in appropriate technology.
 
Originality/value:The study is distinguished that it addresses the role of forensic accounting in improving the suitability and credibility of financial reports and business sustainability in the Kingdom of Saudi Arabia through cooperation with educational institutions in establishing the concept of Forensic accounting and the best use of technology.
O IMPACTO DA COVID-19 EM PARQUE URBANO DO AGRESTE PERNAMBUCANO
Authors: Rocha Lucas de Lucena , Vigoderis Ricardo Brauer , Silva João Manoel da , Lima Aymê Fernanda de Almeida Melo de , Silva José Robson Alves Ferreira da , Leite Glaudemir Santos , Silva Rodriga Jeocona , Pachêco Carla Renata Xavier
Published: March 2024

Objetivo: Analisar as modificações feitas em Parques Urbanos como o Parque Euclides Dourado, localizado no município de Garanhuns/PE, a fim de proporcionar seu uso com segurança em períodos de contágio de doenças como na pandemia da covid-19.
 
Referencial Teórico: Os Parques Urbanos se apresentam como alternativas viáveis para a realização de atividade física, e para restauração da fadiga mental. Com a pandemia da covid-19, as dinâmicas de uso foram modificadas para mitigação de contágio.
 
Método: Para a elaboração da pesquisa de campo, utiliza-se a metodologia de contagem e registro da vida urbana do livro How to Study Public life de Jan Gehl e Birgitte Svarre (2013). A metodologia consiste na contagem; elaboração de estudo de fluxos e percursos de pedestres; mapeamento e registro fotográfico, realizados através de pesquisa de campo.
 
Resultados: Constatou-se que estudos capazes de mensurar a eficácia da mitigação de contágio ainda são escassos e por isso é necessário o desenvolvimento de tecnologias e metodologias específicas para o avanço no campo científico nessa área de estudo.
 
Implicações da pesquisa: Os resultados deste trabalho são de grande relevância para o desenvolvimento sustentável não só apenas do Parque Euclides Dourado, mas sim de todos os Parques Urbanos, já que são capazes de proporcionar a prática de atividade física, relaxamento e biofilia.
 
Originalidade/valor: A compreensão das modificações das dinâmicas de equipamentos públicos, como os parques urbanos, pode fazer com que se as cidades estejam mais preparadas para o enfrentamento de crises sanitárias semelhantes, no futuro.
Color preferences among selected adults in Ghana
Authors:   CSIR-Institute for Scientific and Technological Information, Accra, Ghana, Adjah John , Bekoe Stephen ,   CSIR-Institute for Scientific and Technological Information, Accra, Ghana, Decardi-Nelson Agnes ,   CSIR-Institute for Scientific and Technological Information, Accra, Ghana, Ry-Kottoh Lucy Afeafa ,   Kwame Nkrumah University of Science and Technology, Department of Publishing  Studies, Kumasi, Ghana, Kalognia Joshua ,   CSIR-Institute for Scientific and Technological Information, Accra, Ghana
Published: March 2024

This paper presents a study on color preferences among a sample of Ghanaian adults. Two surveys were conducted, with a total of 143 participants (50 in Survey 1, and 93 in Survey 2). The participants completed both printed and digital questionnaires to gather data on their color preferences. The results showed that blue was the most preferred color by both males and females in general, but not for specific items. In survey 2, a chi-square test on categorical variables revealed a significant relationship between gender and preference for light, dark, or bright colors (p=0.025), as well as gender and number of preferred colors per personal item (p=0.02). However, no significant relationships were found between gender and change of colors from childhood (p=0.73), gender and number of preferred colors (p=0.204), gender and most preferred colors (p=0.216), age, and the number of preferred colors (p=0.19). Interestingly, 66.3% of the participants in Survey 2 indicated that their preferred colors were based on innate attraction, regardless of whether their color preferences had changed or remained the same since childhood. Overall, blue and red were the most preferred colors, with a score of 34.4%. These findings provide valuable insights for design practitioners and communicators and offer a basis for future research on color preferences among Ghanaians.
Modern diagnostic technologies in oncodermatology
Authors: Filonenko E. V. , Kaprin A. D.
Published: March 2024

Skin tumors occupy the first place in terms of incidence in the structure of oncological neoplasms. The WHO estimates that 60,000 people die each year from malignant neoplasms of the skin: 48,000 from melanoma and 12,000 from skin cancer. Timely diagnosis of skin cancer makes it possible to achieve a cure for cancer patients with long periods of relapse-free follow-up after the completion of specialized treatment. The introduction of high-tech optical methods for diagnosing skin neoplasms into clinical practice has significantly increased the specificity, sensitivity, and accuracy of diagnostics. The review is devoted to a discussion of such methods for diagnosing skin neoplasms as fluorescent diagnostics, digital dermatoscopy, SIA-scopy, and confocal microscopy. The features of the application of each of the methods are discussed, the results of the most significant Russian and foreign studies in this field are presented, as well as our own results of the practical application of a number of high-tech optical diagnostic methods at the P.A. Herzen Moscow Oncology Research Center
Impact of steroids on the immune profiles of children with asthma living in the inner-city
Authors: Navalpakam Aishwarya , Thanaputkaiporn Narin , Aijja Crystal , Mongkonsritragoon Wimwipa , Farooqi Ahmad , Huang Jenny , Poowuttikul Pavadee
Published: March 2024

Background: Inner-city asthma is associated with high morbidity and systemic steroid use. Chronic steroid use impacts immune function; however, there is a lack of data with regard to the extent of immunosuppression in patients with asthma and who are receiving frequent systemic
steroids. Objective: To identify the impact of frequent systemic steroid bursts on the immune function of children with asthma who live in the inner city. Methods: Children ages 3‐18 years with asthma were divided into study (≥2 systemic steroid
bursts/year) and control groups (0‐1 systemic steroid bursts/year). Lymphocyte subsets; mitogen proliferation assay; total immunoglobulin G (IgG) value, and pneumococcal and diphtheria/tetanus IgG values were evaluated. Results: Ninety-one participants were enrolled
(study group [n = 42] and control group [n = 49]). There was no difference in adequate pneumococcal IgG value, diphtheria/tetanus IgG value, mitogen proliferation assays, lymphocyte subsets, and IgG values between the two groups. Children who received ≥2 steroid bursts/year had a significantly
lower median pneumococcal IgG serotype 7F value. Most of the immune laboratory results were normal except for the pneumococcal IgG value. Most of the participants (n/N = 72/91 [79%]) had an inadequate pneumococcal IgG level (<7/14 serotypes ≥1.3 µg/mL). The participants with inadequate
pneumococcal IgG level and who received a pneumococcal polysaccharide vaccine 23 (PPSV23) boost had a robust response. There was no significant difference in infection, steroid exposure, asthma severity, or morbidities between those with adequate versus inadequate pneumococcal IgG values.
Conclusion: Children with asthma who live in the inner city and receive ≥2 steroid bursts/year do not have a significantly different immune profile from those who receive ≤1 steroid bursts/year do not have a significantly different immune profile from those who do not.
Although appropriately vaccinated, most participants had an inadequate pneumococcal IgG level, regardless of steroid exposure and asthma severity. These children may benefit from PPSV23.
Total IgE as a biomarker of omalizumab response in chronic spontaneous urticaria: A meta-analysis
Authors: Keller Levi , Perera Ekta K. , Bindon Brittany , Khatiwada Aastha , Stitt Jenny M. , Dreskin Stephen C.
Published: March 2024

Background: Omalizumab is approved for the treatment of chronic spontaneous urticaria (CSU) that is refractory to antihistamines. Total immunoglobulin E (IgE) levels have emerged as a possible biomarker to predict response to omalizumab. However, the existing literature is
heterogenous, with conflicting conclusions with regard to the role of total IgE levels. Objective: We sought to clarify the role of evaluating total IgE levels in patients with CSU by performing a meta-analysis on the existing literature to determine if meaningful changes
exist between responders and nonresponders to omalizumab. Methods: A total of 68 unique citations were returned and screened by two independent reviewers. Editorials, reviews, and case reports were excluded, and a total of 33 original articles were identified and underwent
secondary evaluation. Studies that present mean ± standard deviation total IgE levels and/or 95% confidence intervals (CI) were included, whereas studies with < 25 subjects were excluded. Three studies ultimately met these criteria. Results: We found a mean difference
in total IgE levels between those who responded to omalizumab versus those without a response of 49.76 (95% CI, 7.13‐92.38; p = 0.02), which demonstrated higher mean IgE values in responders compared with nonresponders. Conclusion: This study presents
additional evidence that supports evaluation of total IgE levels as it pertains to response to omalizumab therapy in CSU. When considering the current evidence, it seems reasonable to consider the baseline total IgE level as a biomarker to predict the treatment response to omalizumab. Based
on the existing literature, we cannot conclude at what threshold nonresponse is more likely to occur.
Analisis Karakteristik Dismenore pada Remaja Putri
Authors: Rahmayanti Alfenia , Jafar Fahriani Iatiqamah , Rijai Laode
Published: March 2024

Menstrual pain or dysmenorrhea is pain felt during menstruation so that it can interfere with a woman's daily activities. Usually women experience discomfort during menstruation, such as cramps and are usually accompanied by nausea and dizziness. The aim of this research is to determine the characteristics of young women who experience dysmenorrhea at SMKN 1 Muara Bengkal. This research is descriptive in nature with an observation method. It was found that the incidence of dysmenorrhea occurred most frequently in respondents who were 16 years old at 36.67%, at menarche age 12-13 years at 63.33%, with a duration of menstrual pain of 1 day at 40.00%, respondents did not take care of the pain. amounted to 66.67%, respondents with symptoms of dizziness amounted to 60.00%, and it was found that 76.67% of respondents said that menstrual pain could affect activities. So it can be concluded that there is no relationship between age, age of menarche, duration of menstrual pain, treatment during menstrual pain, other symptoms during dysmenorrhea which can influence the occurrence of dysmenorrhea.

Keywords:          Menstruation, dysmenorrhea, menstrual pain, teenage girls

 

Abstrak

Nyeri haid atau dismenore adalah nyeri yang dirasakan pada saat menstruasi sehingga dapat mengganggu aktifitas sehari-hari wanita. Biasanya wanita mengalami rasa yang tidak nyaman pada saat menstruasi, seperti keram dan biasanya disertai mual dan pusing. Tujuan penelitian ini untuk mengetahui gambaran karakteristik remaja putri yang mengalami dismenore di SMKN 1 Muara Bengkal. Penelitian ini bersifat deskriptif dengan metode observasi. Diperoleh hasil kejadian dismenore paling banyak terjadi pada responden yang memiliki 16 tahun sebesar 36,67%, memiliki usia menarche 12-13 tahun sebesar 63,33%, dengan durasi nyeri haid 1 hari sebesar 40,00%, responden tidak melakukan penanganan saat nyeri sebesar 66,67%, responden dengan gejala pusing sebesar 60,00%, dan didapatkan responden sebesar 76,67% mengatakan bahwa nyeri haid dapat mempengaruhi aktivitas. Sehingga dapat disimpulkan bahwa tidak ada hubungan antara usia, usia  menarche, durasi nyeri haid, penanganan saat nyeri haid, gejala lain saat dismenore yang dapat mempengaruhi terjadinya dismenore.

Kata Kunci:         Menstruasi, dismenore, nyeri haid, remaja putri
Pengaruh Kemasan Penyimpanan terhadap Kadar Senyawa Non Spesifik Kemangi (Ocimum basilicum L.) pada Suhu Tertentu
Authors: Adilah Haani , Sulistiarini Riski , Rahmawati Dewi
Published: March 2024

Basil (Ocimum basilicum L.) contains the most phenolic compounds among other compounds. This condition contributes to the blackening of basil leaves which can reduce the economic value and quality hence the proper storage packaging is needed. This research was conducted to determine the levels of water soluble and ethanol soluble compounds, post-storage weight changes, and qualitative analysis of phenolic compounds. Basil leaves which have been harvested and sorted then packaged in aluminum foil, banana leaves, polypropylene (PP) plastic and newspaper. The storage process is carried out at a temperature of 5-8ºC for 5 days. After storage is complete, tests are carried out to determine the levels of water-soluble and ethanol-soluble compounds, post-storage weight changes, and qualitative analysis of phenolic compounds. The use of packaging influences changes in post-storage weight of basil where banana leaves have the lowest weight loss of 6%. Meanwhile, qualitative analysis of the compounds in the treatment showed that they still contained phenolic compounds.

Keywords:          basil, storage packaging, compounds level

 

Abstrak

Kemangi (Ocimum basilicum L.) mengandung senyawa fenolik paling banyak di antara senyawa lainnya. Kondisi tersebut berperan kepada penghitaman daun kemangi yang dapat menurunkan nilai ekonomi dan kualitas sehingga dibutuhkan kemasan penyimpanan tepat. Penelitian ini dilakukan untuk mengetahui kadar senyawa larut air dan larut etanol, perubahan bobot pasca simpan, dan analisis kualitatif senyawa fenol. Daun kemangi yang telah dipanen dan disortasi kemudian dikemas ke dalam kemasan alumunium foil, daun pisang, plastik polipropilen (PP), dan kertas koran. Proses penyimpanan dilakukan pada suhu 5-8ºC selama 5 hari. Setelah penyimpanan selesai, dilakukan pengujian untuk mengetahui mengetahui kadar senyawa larut air dan larut etanol, perubahan bobot pasca simpan, dan analisis kualitatif senyawa fenol. Penggunaan kemasan berpengaruh terhadap perubahan bobot pasca simpan kemangi dimana daun pisang memiliki susut bobot paling rendah sebesar 6%. Sementara itu, analisis kualitatif senyawa pada perlakuan menunjukkan masih terkandung senyawa fenol.

Kata Kunci:         kemangi, kemasan penyimpanan, kadar senyawa
Penentuan Nilai Sun Protection Factor (SPF) Ekstrak Etanol Rimpang Kunyit Hitam (Curcuma caesia ROXB.) dengan Metode Spektrofotometri UV-Vis
Authors: Ibau Miranda , Sulistiarini Riski , Salam Supriatno
Published: March 2024

Black turmeric (Curcuma caesia ROXB.) is a species of turmeric, the Zingberaceae family which has high antioxidant activity and is closely related to sunscreen activity, which is a compound that absorbs or reflects sunlight so that it can prevent skin disorders. This research aims to determine the Sun Protection Factor (SPF) value of ethanol extract of black turmeric rhizomes using the soxletation extraction method using 96% ethanol solvent and determining the SPF value using the UV-Vis spectrophotometric method. Tests were carried out using varying extract concentrations of 100 ppm, 150 ppm, 200 ppm, 250 ppm and 300 ppm measured at a UV wavelength of 290-320 nm. The research results showed that the SPF values ??of the 100 ppm, 150 ppm, 200 ppm, 250 ppm and 300 ppm concentration series were 10,93; 10,90; 13,49; 19,31; 20,42. Based on these results, it was concluded that the high SPF values ??were found at concentrations of 250 ppm and 300 ppm, namely 19,31 and 20,42 which were included in the ultra protection category.

Keywords:          Black Turmeric, SPF, UV-Vis Spectrophotometry

 

Abstrak

Kunyit hitam (Curcuma caesia ROXB.) merupakan salah satu spesies dari kunyit, dari family Zingberaceae yang memiliki aktivitas antioksidan yang tinggi dan memiliki hubungan erat dengan aktivitas tabir surya yang merupakan senyawa untuk menyerap atau memantulkan sinar matahari sehingga dapat mencegah gangguan pada kulit. Penelitian ini bertujuan untuk mengetahui nilai Sun Protection Factor (SPF) ekstrak etanol rimpang kunyit hitam dengan metode ekstraksi soxletasi menggunakan pelarut etanol 96% dan penentuan nilai SPF menggunakan metode spektrofotometri UV-Vis. Pengujian dilakukan dengan menggunakan variasi konsentrasi ekstrak sebesar 100 ppm, 150 ppm, 200 ppm, 250 ppm dan 300 ppm yang diukur pada panjang gelombang UV 290-320 nm. Hasil penelitian menunjukkan bahwa nilai SPF dari seri konsentrasi 100 ppm, 150 ppm, 200 ppm, 250 ppm dan 300 ppm berturut-turut 10,93; 10,90; 13,49; 19,31; 20,42. Berdasarkan hasil tersebut disimpulkan bahwa nilai SPF yang tinggi terdapat pada konsentrasi 250 ppm dan 300 ppm yakni 19,31 dan 20,42 yang termasuk dalam kategori proteksi ultra.

Kata Kunci:         Kunyit Hitam, SPF, Spektrofotometri UV-Vis
Karakteristik Ekstrak Daun Kersen (Muntingia calabura L.) sebagai Kandidat Bahan Aktif dalam Formulasi Gel Sleeping Mask
Authors: Islamika Mirda Fitria , Sobah Nurus , Narsa Angga Cipta
Published: March 2024

Kersen plant (Muntingia calabura L.) is one of the plants that has antioxidant activity. The phytochemical content in kersen plants includes flavonoids, terpenoids, steroids, phenolics, saponins and tannins. This content makes kersen plants have high antioxidant activity and potential to be formulated in the form of sleeping mask gel. Antioxidants have an important role that can neutralize free radicals in the body, where these free radicals are closely related to accelerated aging and carcinogenic. Therefore, the antioxidant activity of kersen leaf extract was tested quantitatively using a UV-Vis spectrophotometer to determine the potential of kersen plants as a source of antioxidants. The results showed that kersen plant has characteristics of blackish brown color, solid form, distinctive aroma, with an extract pH of 4.29. The content of secondary metabolite compounds in it include flavonoids, alkaloids, and tannins. The results of the antioxidant activity test of kersen leaf extract showed an IC50 value of 13.11242 and was included in the very strong category.

Keywords:          Kersen Leaf, Extract, Antioxidant

 

Abstrak

Tumbuhan kersen (Muntingia calabura L.) merupakan salah satu tumbuhan yang memiliki aktivitas antioksidan. Kandungan fitokimia dalam tanaman kersen meliputi flavonoid, terpenoid, steroid, fenolik, saponin dan tanin. Kandungan ini menjadikan tumbuhan kersen memiliki aktivitas antioksidan yang tinggi dan berpotensi untuk diformulasikan dalam bentuk sediaan gel sleeping mask. Antioksidan memiliki peran penting yaitu dapat menetralkan radikal bebas yang ada di dalam tubuh, dimana radikal bebas ini berkaitan erat dengan percepatan penuaan dan karsinogenik. Oleh karena itu, dilakukan pengujian aktivitas antioksidan ekstrak daun kersen secara kuantitatif menggunakan spektrofotometer UV-Vis untuk mengetahui potensi tumbuhan kersen sebagai sumber antioksidan. Hasil penelitian menunjukkan bahwa tumbuhan kersen memiliki karakteristik warna coklat kehitaman, berwujud padat, aroma khas, dengan pH ekstrak 4,29. Kandungan senyawa metabolit sekunder yang ada di dalamnya antara lain flavonoid, alkaloid, dan tanin. Hasil uji aktivitas antioksidan ekstrak daun kersen  menunjukkan nilai IC50 sebesar 13.11242 dan termasuk dalam kategori sangat kuat.

Kata Kunci:         Daun Kersen, Ekstrak, Antioksidan
Optimasi Pembuatan Nanopartikel Ekstrak Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) Berdasarkan Konsentrasi Kitosan
Authors: Liranti Nintra Mahdan , Burhanudin Muhammad , Sastyarina Yurika
Published: March 2024

Dayak onion extract (Eleutherine bulbosa (Mill.) Urb.) is known to have strong antibacterial activity so it has the potential to be developed into a nanoparticle delivery system. Chitosan polymer has the advantage of delivering active substances because it has good stability, low toxicity, and the preparation method is quite simple. This research aims to determine the effect of variations in chitosan–tripolyphosphate (TPP) concentration on the size and polydispersity index of nanoparticles. Dayak onion extract was formulated in the form of nanoparticles using the ionic gelation method with varying concentrations of chitosan polymer: tripolyphosphate 0.1%, 0.2%, 0.3% and 0.4%. Test parameters include determining the size and polydispersity index of nanoparticles using a particle size analyzer. The results showed that F1, F2, F3, and F4 had particle sizes of 376.6 nm, 354.7 nm, 480.1 nm, 654.0 nm, respectively, and a polydispersity index of 1.666; 1,702; 0.949; and 1,585 with heterogeneous size distribution. Based on the results of research that has been carried out, it can be concluded that Dayak onion extract can be formulated in nanoparticle sizes with varying physical characteristics depending on the concentration of chitosan and tripolyphosphate used.

Keywords:          Dayak onion (Eleutherine bulbosa (Mill.) Urb.), nanoparticles, chitosan, tripolyphosphate, ionic gelation

 

Abstrak

Ekstrak bawang dayak (Eleutherine bulbosa (Mill.) Urb.) diketahui memiliki aktivitas antibakteri yang kuat sehingga berpotensi untuk dikembangkan menjadi sistem penghantaran nanopartikel. Polimer kitosan memiliki kelebihan untuk menghantarkan zat aktif karena memiliki stabilitas yang baik, toksisitas yang rendah, dan metode preparasinya yang cukup sederhana. Penelitian  ini  bertujuan  untuk  mengetahui pengaruh  variasi konsentrasi  kitosan–tripolifosfat  (TPP)  terhadap  ukuran dan indeks polidisperitas  nanopartikel. Ekstrak bawang dayak diformulasi dalam bentuk nanopartikel dengan metode gelasi ionik dengan  variasi  konsentrasi  polimer  kitosan : tripolifosfat 0,1%, 0,2%, 0,3%, dan 0,4%. Parameter pengujian meliputi penentuan ukuran dan indeks polidispersitas  nanopartikel  menggunakan particle  size  analyzer. Hasil penelitian menunjukkan bahwa F1, F2, F3, dan F4 memiliki ukuran partikel masing-masing sebesar 376,6 nm, 354,7 nm, 480,1 nm, 654,0 nm, dan indeks polidisperisitas masing-masing 1,666; 1,702; 0,949; dan 1,585 dengan distribusi ukuran yang heterogen. Berdasarkan hasil penelitian yang telah dilakukan, dapat disimpulkan bahwa ekstrak bawang dayak dapat diformulasi   dalam   ukuran   nanopartikel   dengan karakteristik fisik yang bervariasi tergantung pada konsentrasi kitosan dan tripolifosfat yang digunakan.

Kata Kunci:         Bawang dayak (Eleutherine bulbosa (Mill.) Urb.), nanopartikel, kitosan, tripolifosfat, gelasi ionik
Karakteristik Teh Herbal Daun Kersen (Muntingia calabura L.) dan Daun Salam (Syzygium polyanthum) Sebagai Antioksidan
Authors: Nurfitri Nurfitri , Herman Herman , Arief Maryam Jamila
Published: March 2024

Antioxidants can protect body cells from damage caused by free radicals. Free radicals can damage cells and body tissue. The body has natural antioxidants but not in large quantities so the body needs antioxidants that come from outside. Antioxidants from outside the body can come from various natural ingredients native to Indonesia which have potential as antioxidants, one of which comes from cherry leaves (Muntingia calabura L.) and bay leaves (Syzygium polyanthum). This research aims to determine the antioxidant activity of cherry leaf (Muntingia calabura L.) and bay leaf (Syzygium polyanthum) herbal tea so that people can use it as an alternative source of antioxidants. The data collection method is carried out using instruments in the laboratory. The research results showed that cherry leaves (Muntingia calabura L.) and bay leaves (Syzygium polyanthum) were identified as containing alkaloids, flavonoids, saponins, tannins and terpenoids. Simplicia's characteristic data meets the requirements of SNI (Indonesian National Standards). As well as the IC50 value of herbal tea, cherry leaves (Muntingia calabura L.) were found to be 1,39 µg/ml and bay leaves (Syzygium polyanthum) 1,72 µg/ml, which means they have very strong antioxidant activity because the IC50 value is <50.

Keywords:          Characteristics, Herbal tea of ??cherry leaves (Muntingia calabura L.) and bay leaves (Syzygium polyanthum), Antioxidants, IC50 value

 

Abstrak

Antioksidan dapat melindungi sel tubuh dari kerusakan yang disebabkan radikal bebas. Radikal bebas dapat merusak sel serta jaringan tubuh. Tubuh memiliki antioksidan alami tetapi tidak dalam jumlah besar sehingga tubuh membutuhkan antioksidan yang berasal dari luar. Antioksidan dari luar tubuh dapat berasal dari berbagai macam bahan alam asli Indonesia yang memiliki potensi sebagai antioksidan, salah satunya berasal dari daun kersen (Muntingia calabura L.) dan daun salam (Syzygium polyanthum). Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dari teh herbal daun kersen (Muntingia calabura L.) dan daun salam (Syzygium polyanthum) agar dapat dimanfaatkan masyarakat sebagai sumber alternatif antioksidan. Metode pengumpulan data dilakukan menggunakan instrumen di laboratorium. Hasil penelitian menunjukkan bahwa daun kersen (Muntingia calabura L.) dan daun salam (Syzygium polyanthum) teridentifikasi mengandung alkaloid, flavonoid, saponin, tannin, dan terpenoid. Data karakteristik simplisia telah memenuhi syarat SNI (Standar Nasional Indonesia). Serta nilai IC50 dari teh herbal didapatkan daun kersen (Muntingia calabura L.) yaitu 24,9344 µg/ml dan daun salam (Syzygium polyanthum) 48,9666 µg/ml yang berarti memiliki aktivitas antioksidan sangat kuat karena nilai IC50 nya <50.

Kata Kunci:         Karakteristik, Teh herbal daun kersen (Muntingia calabura L.) dan daun salam (Syzygium polyanthum), Antioksidan, nilai IC50.
Isolasi dan Karakterisasi Jamur Endofit Daun Belimbing Hutan (Cnestis palala (Lour.) Merr)
Authors: Azzahra Nurvidya Sistha , Naspiah Nisa , Febrina Lizma
Published: March 2024

Belimbing hutan (Cnestis palala (Lour.) Merr) is recognized as a distinctive plant in East Kalimantan, commonly used by the local community for treating internal diseases, postpartum conditions, abdominal pain, and urinary tract disorders. The benefits of belimbing hutan cannot be separated from the compounds contained in it. However, the limited population makes exploration of this plant hampered. Endophytic fungi are an interesting alternative for exploring secondary metabolites from this plant. To date, there have been no scientific reports on endophytic fungus of belimbing hutan. This research successfully isolated and characterized three isolates from the leaves of belimbing hutan with isolate codes IJP1 exhibiting white colonies with a cotton-like texture, IJP2 colonies are white and fiber-shaped, IJH1 with green colonies accompanied by concentric circles. The diversity of endophytic fungi in belimbing hutan leaves opens up possibilities for discovering potential compounds from the extracts of these fungi.

Keywords:          Cnestis palala (Lour.) Merr, Endophytic Fungi, Isolation, Characterization

 

Abstrak

Belimbing hutan (Cnestis palala (Lour.) Merr) dikenal sebagai tanaman khas Kalimantan Timur yang umumnya digunakan sebagai obat penyakit dalam, obat pasca melahirkan, sakit perut dan gangguan saluran kemih oleh masyarakat sekitar. Khasiat dari belimbing hutan ini tidak terlepas dari senyawa yang terkandung di dalamnya. Namun, populasi yang terbatas membuat eksplorasi tumbuhan ini menjadi terhambat. Jamur endofitik merupakan salah satu alternatif menarik untuk mengeksplorasi metabolit sekunder dari tanaman ini. Sepanjang pengetahuan, belum ada laporan ilmiah mengenai jamur endofitik belimbing hutan. Penelitian ini berhasil mengisolasi dan mengkarakterisasi tiga isolat dari daun belimbing hutan dengan kode isolat IJP1 yang memiliki karakteristik koloni berwarna putih dan tekstur halus seperti kapas, IJP2 koloni berwarna putih dan berbentuk serabut, IJH1 koloni berwarna hijau disertai lingkaran konsentris. Beragamnya jamur endofitik daun belimbing hutan membuka peluang ditemukannya senyawa potensial dari ekstrak jamur endofitik daun belimbing hutan.

Kata Kunci:         Cnestis palala (Lour.) Merr, Jamur Endofit, Isolasi, Karakteristik