bluuebunny/crossref_metadata_2025 huggingface dataset:
Sympathetic innervation of interscapular brown adipose tissue is not a predominant mediator of Oxytocin (OT)-elicited reductions of body weight gain and adiposity in male diet-induced obese rats
Authors: Edwards Melise M. , Nguyen Ha K. , Dodson Andrew D. , Herbertson Adam J. , Honeycutt Mackenzie K. , Slattery Jared D. , Rambousek June R. , Tsui Edison , Wolden-Hanson Tami , Wietecha Tomasz A. , Graham James L. , Tapia Geronimo P. , Sikkema Carl L. , O’Brien Kevin D. , Mundinger Thomas O. , Peskind Elaine R. , Ryu Vitaly , Havel Peter J. , Khan Arshad M. , Taborsky Gerald J. , Blevins James E.
Published: December 2024

Recent studies indicate that central administration of oxytocin (OT) reduces body weight (BW) in high fat diet-induced obese (DIO) rodents by reducing energy intake and increasing energy expenditure (EE). Previous studies in our lab have shown that administration of OT into the fourth ventricle (4V; hindbrain) elicits weight loss and stimulates interscapular brown adipose tissue temperature (TIBAT) in DIO rats. We hypothesized that OT-elicited stimulation of sympathetic nervous system (SNS) activation of IBAT contributes to its ability to activate BAT and reduce BW in DIO rats. To test this, we determined the effect of disrupting SNS activation of IBAT on OT-elicited stimulation of TIBAT and reduction of BW in DIO rats. We first confirmed that bilateral surgical SNS denervation to IBAT was successful based on having achieved ≥60% reduction in IBAT norepinephrine (NE) content from DIO rats. NE content was selectively reduced in IBAT by 94.7 ± 2.7, 96.8% ± 1.8% and 85.9% ± 6.1% (p < 0.05) at 1, 6 and 7 week post-denervation, respectively, and was unchanged in liver or inguinal white adipose tissue. We then measured the impact of bilateral surgical SNS denervation to IBAT on the ability of acute 4V OT (1, 5 µg) to stimulate TIBAT in DIO rats. We found that the high dose of 4V OT (5 µg) stimulated TIBAT similarly between sham and denervated rats (P=NS) and that the effects of 4V OT to stimulate TIBAT did not require beta-3 adrenergic receptor signaling. We subsequently measured the effect of bilateral surgical denervation of IBAT on the effect of chronic 4V OT (16 nmol/day) or vehicle infusion to reduce BW, adiposity, and energy intake in DIO rats. Chronic 4V OT reduced BW gain by −7.2 ± 9.6 g and −14.1 ± 8.8 g in sham and denervated rats (p < 0.05 vs. vehicle treatment), respectively, and this effect was similar between groups (P=NS). These effects were associated with reductions in adiposity and energy intake (p < 0.05). Collectively, these findings support the hypothesis that sympathetic innervation of IBAT is not required for central OT to increase BAT thermogenesis and reduce BW gain and adiposity in male DIO rats.
Evolution of the overlying strata structure and characteristics of ground pressure behavior under the influence of tectonic stress
Authors: Zhu Zhijie , Wang Peng , Chen Kun , Lv Fei , Hong Yin
Published: December 2024

The evolution of overlying strata structures significantly influences the manifestation of ground pressure behavior in working faces. The mechanism through which tectonic stress influences the evolution of overlying rock structures remains unclear. To address this, we established a nonlinear dynamic mathematical-mechanical model to analyse and propose a theory for the structural evolution of overlying rock structures under tectonic stress. Based on the 8,105 working face in Tongxin Mine, China, we used theoretical calculations and numerical simulations to analyse the influence of tectonic stress on the formation and evolution of the overlying rock “plate-shell” structure. Under similar coal and rock seam distribution conditions, higher tectonic stress led to larger first failure interval of the key stratum. Consequently, the scale of the “plate-shell” structure controlled by the key stratum increased, resulting in more pronounced ground pressure behavior. The EH-4 geomagnetic method and numerical simulations indicated that the damage height of the overlying strata at the working face was 170 m. In response to the intense manifestation of mineral pressure resulting from large-scale overlying strata structure above the working face, the implementation of pre-cracking blasting can significantly reduce the failure interval of the key stratum and alleviate surrounding rock stress.
Role of rapidly evolving immunotherapy in chronic active Epstein-Barr virus disease
Authors: Yan Sijia , Ming Xi , Zhu Xiaojian , Xiao Yi
Published: December 2024

Chronic active Epstein-Barr Virus disease is a kind of Epstein-Barr Virus associated T/NK cell lymphoproliferative disease. At present, there is still a lack of standard therapeutic regimen for its treatment, but its basic treatment principles include controlling inflammatory response, anti-tumor proliferation, and immune reconstitution. Hematopoietic stem cell transplantation is currently the only method that can cure this disease. In recent years, immunotherapy has developed rapidly and is widely used in the treatment of various hematological malignancies; various immunotherapy drugs, including PD-1 inhibitors, have also demonstrated their safety and efficacy in CAEBV, while immune cell therapies such as Epstein- Barr virus-specific T cells have also displayed their unique advantages in CAEBV.
Aseptic abscess syndrome: a case report of a patient achieving remission with both infliximab originator and biosimilar administered at varied intervals
Authors: Ucci Federica Maria , Scrivo Rossana , Alessandri Cristiano , Conti Fabrizio , Priori Roberta
Published: December 2024

Aseptic abscesses syndrome is a rare but increasingly recognized disease that falls within the spectrum of autoinflammatory disorders. Here, we describe the case of a patient who presented with abdominal pain and fever, along with multiple abdominal and extra-abdominal abscesses, in the absence of underlying hematologic, autoimmune, infectious, or neoplastic conditions. Initially, the patient responded to glucocorticoids, but experienced several flares upon discontinuation, leading to the initiation of treatment with a TNFα inhibitor. After 5 years, an attempt to discontinue treatment resulted in a new flare of the disease. Remission was eventually achieved with a biosimilar TNFα inhibitor, albeit requiring shortened infusion intervals.
Genetic insights into the effect of trace elements on cardiovascular diseases: multi-omics Mendelian randomization combined with linkage disequilibrium score regression analysis
Authors: Chen Bohang , Wang Chuqiao , Li Wenjie
Published: December 2024

Objective

Epidemiological evidence indicates that trace elements are significantly associated with cardiovascular health. However, its causality and underlying mechanisms remain unclear. Therefore, this study aimed to investigate the causal relationship between trace elements and cardiovascular disease, as well as their potential mechanism of action.

Method

Two-sample Mendelian randomization (MR) analyses along with mediated and multivariate MR analyses were employed. These analyses utilized 13 trace elements as exposure variables and 20 cardiovascular diseases as outcome variables, with 4907 circulating plasma proteins, 1400 serum metabolites, 731 immune cell phenotypes, and 473 intestinal flora as potential mediators. The Bayesian weighted MR method was used to validate the MR results, and linkage disequilibrium score regression (LDSC) was applied to explore the genetic correlation between trace elements and cardiovascular disease.

Result

Our findings indicated a positive or negative causal relationship between genetically predicted trace elements and cardiovascular disease. An analysis using the Bayesian weighted MR method demonstrated that our causal inference results were reliable. The results of the mediated MR analyses indicate that potassium may reduce the risk of ischemic heart disease by influencing the expression of the plasma proteins BDH2 and C1R. Vitamin B12 may increase the risk of coronary atherosclerosis and cardiovascular death by reducing the levels of VPS29 and PSME1 proteins, while vitamin C may mitigate the risk of cardiac arrest by inhibiting the expression of the TPST2 protein. In addition, potassium can reduce the risk of ischemic heart disease by lowering 4-methoxyphenyl sulfate levels. None of the instrumental variables exhibited pleiotropy in the MR analysis. A sensitivity analysis using the leave-one-out method further confirmed the robustness of our findings. LDSC results indicated a genetic correlation between multiple trace elements and various cardiovascular diseases.

Conclusion

This study uncovered the true causal relationship between trace elements and cardiovascular disease risk using genetic methods, and revealed the significant mediating role of specific plasma proteins and metabolites in this relationship.
Epigenetic regulation of complement C1Q gene expression
Authors: Pegoraro Silvia , Balduit Andrea , Mangogna Alessandro , Kishore Uday , Ricci Giuseppe , Agostinis Chiara , Bulla Roberta
Published: December 2024

Human C1q is a multifaceted complement protein whose functions range from activating the complement classical pathway to immunomodulation and promoting placental development and tumorigenesis. It is encoded by the C1QA, C1QB, and C1QC genes located on chromosome 1. C1q, unlike most complement components, has extrahepatic expression by a range of cells including macrophages, monocytes and immature dendritic cells. Its local synthesis under the conditions of inflammation and for the purpose of removal of altered self requires its strict transcriptional regulation. To delve into C1Q transcriptional regulation and unravel potential epigenetic influences, we conducted an in silico analysis utilizing a range of online tools and datasets. Co-expression analysis revealed tight coordination between C1QA, C1QB, and C1QC genes. Strikingly, distinct epigenetic patterns emerged across various cell types expressing or lacking these genes, with unique histone marks and DNA methylation status characterizing their regulatory landscape. Notably, the investigation extended to tumor contexts, unveiled potential epigenetic roles in malignancies. The cell type and tumor-specific histone modifications and chromatin accessibility patterns underscore the dynamic nature of epigenetic regulation of C1Q, providing crucial insights into the intricate mechanisms governing the expression of these immunologically significant genes. The findings provide a foundation for future investigations into targeted epigenetic modulation, offering insights into potential therapeutic avenues for immune-related disorders and cancer mediated via C1q.
Humpback whales (Megaptera novaeangliae) in Hervey Bay, Australia: a stopover for females early in their southern migration
Authors: Brooks Lyndon , Franklin Trish , Franklin Wally , Harrison Peter , Corkeron Peter , Pollock Kenneth H.
Published: December 2024

In the Southern Hemisphere, humpback whales (Megaptera novaeangliae) migrate along the extended continental coastlines of Australia, South America, and South Africa. This study reports on photo-identification capture–recapture data from a long-term survey conducted in Hervey Bay, Queensland, where a substantial proportion of the population stop over early in the southern migration. Photo-identification data were collected over 10 weeks per year from 1997 to 2009. The migration through Hervey Bay is dominated and led by females with high fidelity to the site. Mature females, yearlings, and immature whales use the Bay during August, while mature lactating females with calves dominate during September and October. Complex social behaviours occur throughout the season and differ between the early and late cohorts. We argue that the composition of the two cohorts and their distinctively different behaviours indicate that Hervey Bay is not simply a resting site but an area of aggregation that serves important social and biological benefits. A multistate open robust design model was fitted to capture–recapture data to estimate the annual number of whales visiting the Bay, the permanent emigration rate, proportions of the visiting population that do not enter the Bay each year, the number present during each week, and their residency times. The number of annual visitors to the Bay increased approximately linearly from 857 in 1997 to 2175 at the end of sampling in 2009 with two-thirds migrating through during the first half of each season. The population rate of growth may have been slowing by 2009, but there was considerable uncertainty in the trajectory and little basis for projection into the future. While it is desirable to know the current status of the Hervey Bay population and what has occurred since 2009, the cost and effort required make further manual collection and matching of images unlikely. The development of AI algorithmic matching software may enable further research in future.
Holocene evolution of the buried tidal sand body in the North Jiangsu Plain of China revealed by luminescence dating
Authors: Nian Xiaomei , Zhang Weiguo , Wang Xuemei , Qiu Fengyue
Published: December 2024

In the northern Jiangsu coastal zone of China, the buried tidal sand body (BTSB) is suggested to share a similar origin with the offshore radial sand ridge system in the southwestern Yellow Sea. However, its chronological framework remains inadequately understood. This study conducted optically stimulated luminescence (OSL) dating of both silt- and sand-sized quartz on core LDC from the southwestern end of the BTSB. Together with data from the previously studied core XYK closer to the current coastline, this study aims to clarify the chronology of the BTSB and refine its evolution history. The results indicate that in both cores, sand-sized quartz provides more reliable age estimates than silt-sized quartz for the sandy sediment layers. Additionally, the discrepancy between ages derived from the single-grain central age model and the minimum age model is smaller within the top 11 m of the core, which was deposited over the last 0.9 ka. This period corresponds well with the southern migration of the Yellow River and its sediment discharge into the Yellow Sea from 1128 to 1855 CE. It suggests that distinct sediment sources from the Yellow and Yangtze Rivers may account for the observed differences in OSL characteristics. The OSL ages reveal significant temporal variations in sedimentation rates during the Holocene, with the most rapid deposition occurring between 1.2–0.4 ka and 10–8 ka in core LDC, and between 2–1 ka in core XYK. Together with dating results from the central part of the BTSB, it reveals complex spatiotemporal variations in sediment accumulation and emphasizes the need for detailed sediment sampling and dating to fully elucidate the evolutionary history of the coastal plain.
Qualitative study on the ability of neurological nurses to manage patients with indwelling gastrointestinal canal
Authors: Wang Zezhou , Liu Yan , Fang Kui , Hao Xixi , Xue Zhihan , Dong Xue , Wang Haijun
Published: December 2024

Aim and objectives

This study aims to assess the proficiency of nurses in the neurology department in managing patients with indwelling gastrointestinal canal.

Background

Many critically ill patients in neurology departments require the placement of nasoenteric tubes to provide enteral nutrition. However, in clinical practice, there exists considerable variability in nurses’ competencies regarding the management of patients with nasoenteric tubes. A thorough understanding of the challenges nurses face throughout the entire process of nasoenteric tube care and the development of targeted improvement measures are crucial for delivering enhanced patient care and facilitating patient recovery.

Design

A qualitative interview study.

Methods

From August to October 2023, 11 neurological nurses were recruited offline in three hospitals for semi-structured and in-depth interviews. The data analysis uses the KJ method to summarize and classify the themes from the semi-structured interview results, and then organize the themes into a coherent visual and logical path. The COREQ list is used.

Results

The researchers conducted semi-structured interviews with 11 neurology nurses from three hospitals using a purposive sampling method, of which 64% of the participants were female and 36% were male; the average age was 38.27 ± 7.85 years; 27% from the Department of Neurology, 73% from the Department of Neurosurgery; junior professional titles accounted for 18%, intermediate professional titles accounted for 64%, senior professional titles accounted for 18%; 27% of the subjects were head nurses and 73% were nurses. The study identified 5 themes: (1) Preparation and evaluation before intubation; (2) Enhancement of intubation success rates; (3) Prevention of complications; (4) Management of complications and emergencies; (5) The acquisition of relevant knowledge and experience. In clinical practice, the bedside blind insertion technique is random and has not yet formed a unified standard, and the specific operation details and techniques need to be further improved.

Conclusion

Disparities exist in the nursing capabilities of neurology nurses due to variations in their knowledge and experience regarding gastrointestinal canal management.
Acute respiratory distress syndrome caused by Chlamydia psittaci: a case report and literature review
Authors: Zhou Yan , Zou Ya , Zhou Lu , Wei Hua , Zou Yong-Wei , Guo Xi-Rui , Ye Yong-Qin , Li Na , Lu Yun
Published: December 2024

Background

Psittacosis is a zoonotic disease with a low incidence rate and a lack of specificity in clinical manifestations, making it prone to be missed, misdiagnosed, and even cause delayed treatment for patients. Metagenomic next-generation sequencing (mNGS) was successfully performed for the diagnosis of a young patient with psittacosis progressing to acute respiratory distress syndrome (ARDS), and precisely targeted antibiotic treatment was promptly administered. Additionally, a comprehensive review was conducted on 68 cases of psittacosis complicated with ARDS, with the goal of improving the clinical awareness of this disease.

Case presentation

This study reports a 37-year-old young female who was infected with Chlamydia psittaci (C. psittaci) after contact with parrots and eventually developed ARDS. The patient initially developed fever and sore throat, followed by cough and expectoration. Despite receiving empirical anti-infection treatment, the condition continued to progress rapidly, and severe dyspnea developed within a short period of time. She was subsequently transferred to the intensive care unit (ICU) and underwent tracheal intubation and mechanical ventilation due to acute respiratory failure. After the DNA sequence of C. psittaci in bronchoalveolar lavage fluid (BALF) was detected through mNGS, the patient received targeted antibiotic treatment with doxycycline and moxifloxacin, and her clinical symptoms gradually improved.

Conclusion

Epidemiological investigations and the application of mNGS are crucial for the early identification and diagnosis of psittacosis. For suspected psittacosis patients, the application of mNGS technology could promote early identification of pathogens and targeted antimicrobial therapy, which might improve patient prognosis. In addition, young psittacosis patients without underlying disease should also be vigilant about the possibility of developing severe cases.
Microbiota dynamics and source tracing during the growing, aging, and decomposing processes of Eucommia ulmoides leaves
Authors: Shao Qiuyu , Dong Chunbo , Han Yanfeng , Zhang Yanwei
Published: December 2024

Eucommia ulmoides, an important tree, faces serious threat to its growth from environmental stress, particularly climate change. Using plant microbes to enhance host adaptation to respond climate change challenges has been recognized as a viable and sustainable strategy. However, it is still unclear how the perennial tree microbiota varies across phenological stages and the links between respective changes in aboveground and belowground niches. Here, we sequenced 27 root and 27 leaf samples of E. ulmoides using 16S rRNA and ITS amplicon sequencing techniques. These samples were obtained from the three main phenological stages of leaves, including leaf growing, aging and decomposing stages. Results showed that the diversity, composition, and function of the leaf microbiota of E. ulmoides showed more obvious changes at three phenological time points compared to roots. Regarding alpha diversity, the root microbiota showed no difference across three sampling stages, while the leaf microbiota varied with sampling stages. Regarding beta diversity, the root microbiota clustered from different sampling stages, while the leaf microbiota exhibited distinct separation. Regarding composition and function, the dominant taxa and main functions of the root microbiota were the same in three sampling stages, while the leaf microbiota in the decomposing stage was obviously different from the remaining two stages. Additionally, taxa overlap and source-sink relationship existed between E. ulmoides microbiota. Specifically, the degree of overlap among root microbiota was higher than that of leaf microbiota in three sampling stages. The bidirectional source-sink relationship that existed between the root and leaf niches varied with sampling stage. During the leaf growing and aging stages, the proportion of microbial members migrating from roots to leaves was higher than the proportion of members migrating from leaves to roots. During the leaf decomposing stage, the migration characteristics of the fungal community between the root and leaf niches maintained the same as in the remaining two stages, but the proportion of bacterial members migrating from leaves to roots was significantly higher than that of members migrating from roots to leaves. Our findings provide crucial foundational information for utilizing E. ulmoides microbiota to benefit their host under climate change challenges.
Associations of posttraumatic stress disorder symptoms with amyloid burden in cognitively normal older adults
Authors: Zhang Lei , Gong Yi-Miao , Wang San-Wang , Shi Pei-Ling , Li Ming-Zhe , Wen Xin , Wang Di-Xin , Zheng Yong-Bo , Han Yong
Published: December 2024

Background

Posttraumatic stress disorder (PTSD) is associated with the development of dementia. However, the link between PTSD and preclinical Alzheimer’s disease pathology (amyloid β [Aβ]) remains controversial. Moreover, the correlation between the severity of PTSD with Aβ levels remains unknown.

Methods

This cross-sectional study sought to investigate the associations of PTSD symptoms with global and regional brain Aβ burden. To this end, data were obtained from participants in the Anti-Amyloid Treatment in Asymptomatic Alzheimer’s Disease (A4) Study. In addition, we explored the association between the severity of PTSD symptoms and Aβ levels.

Results

A total of 4,228 participants aged 65 to 85 years were included in the final analysis. The results showed that PTSD symptoms were significantly associated with higher global Aβ levels (1.15 ± 0.20 vs. 1.09 ± 0.19; β = 0.056; p < 0.001), after adjusting for covariates. The association between PTSD symptoms and Aβ levels was not affected by sex, age, ApoE genotype, or psychiatric diseases. Similarly, PTSD symptoms were significantly associated with Aβ levels in all subregions, including the anterior cingulate, posterior cingulate, parietal cortex, precuneus, temporal cortex, and frontal cortex. In addition, the group with severe PTSD symptoms (1.22 ± 0.24) exhibited higher global Aβ levels than the groups with moderate (1.14 ± 0.19) or mild (1.12 ± 0.20) symptoms or the control (1.08 ± 0.18), with p < 0.001.

Conclusion

The findings imply a close relationship between PTSD and brain Aβ levels, irrespective of sex, age, ApoE genotype, or psychiatric diseases. More well-designed studies are needed to further explore the relationship and mechanism underlying the association between PTSD and Aβ burden.
Using lifestyle interventions and the gut microbiota to improve PTSD symptoms
Authors: Sugden Steven G. , Merlo Gia
Published: December 2024

Posttraumatic stress disorder is part of a spectrum of psychological symptoms that are frequently linked with a single defining traumatic experience. Symptoms can vary over the lifespan in intensity based on additional life stressors, individual stability, and connectedness to purpose. Historically, treatment has centered on psychotropic agents and individual and group therapy to increase the individual’s window of tolerance, improve emotional dysregulation, and strengthen relationships. Unfortunately, there is a growing segment of individuals with posttraumatic stress disorder who do not respond to these traditional treatments, perhaps because they do not address the multidirectional relationships between chronic cortisol, changes in the brain gut microbiota system, neuroinflammation, and posttraumatic symptoms. We will review the literature and explain how trauma impacts the neuroendocrine and neuroimmunology within the brain, how these processes influence the brain gut microbiota system, and provide a mechanism for the development of posttraumatic stress disorder symptoms. Finally, we will show how the lifestyle psychiatry model provides symptom amelioration.
The role of FDG PET assessment in patients with advanced breast cancer treated with cyclin-dependent kinase 4/6 inhibitors in the second-line setting
Authors: Kubeczko Marcin , Polakiewicz-Gilowska Anna , D’Amico Andrea , Chrabański Olgierd , Świderska Katarzyna , Chmielik Ewa , Blamek Sławomir , Handkiewicz-Junak Daria , Jarząb Michał
Published: December 2024

Background

Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors have demonstrated a survival benefit in the second-line treatment of patients with hormone receptor-positive human epidermal growth factor receptor 2-negative advanced breast cancer. However, identifying prognostic biomarkers remains a challenge. Thus, we aimed to assess the prognostic value of 18F-fluorodeoxyglucose positron emission tomography-computed tomography (FDG-PET-CT) performed before CDK4/6 inhibitors initiation.

Methods

This single-center retrospective analysis comprised patients treated with CDK4/6 inhibitors in the second-line setting between 2018 and 2024, with FDG-PET-CT performed before CDK4/6 inhibitor initiation.

Results

The study included 39 patients with a median age of 63 years (IQR 50 -71). Among them, 12 had de novo metastatic disease (30.8%), and 13 had oligometastatic disease (33.3%). Treatment distribution was as follows: 15 patients received palbociclib (38%), 19 ribociclib (49%), and five abemaciclib (13%). Most patients received fulvestrant (31 patients, 79%), whereas eight patients (21%) were treated with letrozole. The median progression-free survival (PFS) in all studied patients was 25.8 months. Notably, baseline SUVmax (maximum standardized uptake value) showed statistically and clinically significant differences. Patients in the low SUVmax group had a median PFS of 30.7 months, compared to 13.0 months for those in the high SUVmax group (p = 0.038). The 2-year PFS was 76.2% [95% CI 51.8% - 89.4%] for the low SUVmax group, contrasting with 22.3% [95% CI 4.0% - 49.9%] for the high SUVmax group. High SUVmax, poor performance status, and de novo metastatic disease were independent prognostic factors for PFS.

Conclusions

FDG-PET-CT performed before cyclin-dependent kinase 4/6 inhibitor commencement is a valuable prognostic tool in hormone receptor-positive human epidermal growth factor receptor 2-negative advanced breast cancer. Patients with SUVmax less than 8.4 experienced extended progression-free survival compared to those with higher SUVmax.
DenseIncepS115: a novel network-level fusion framework for Alzheimer's disease prediction using MRI images
Authors: Rauf Fatima , Khan Muhammad Attique , Brahim Ghassen Ben , Abrar Wardah , Alasiry Areej , Marzougui Mehrez , Jeon Seob , Nam Yunyoung
Published: December 2024

One of the most prevalent disorders relating to neurodegenerative conditions and dementia is Alzheimer's disease (AD). In the age group 65 and older, the prevalence of Alzheimer's disease is increasing. Before symptoms showed up, the disease had grown to a severe stage and resulted in an irreversible brain disorder that is not treatable with medication or other therapies. Therefore, early prediction is essential to slow down AD progression. Computer-aided diagnosis systems can be used as a second opinion by radiologists in their clinics to predict AD using MRI scans. In this work, we proposed a novel deep learning architecture named DenseIncepS115for for AD prediction from MRI scans. The proposed architecture is based on the Inception Module with Self-Attention (InceptionSA) and the Dense Module with Self-Attention (DenseSA). Both modules are fused at the network level using a depth concatenation layer. The proposed architecture hyperparameters are initialized using Bayesian Optimization, which impacts the better learning of the selected datasets. In the testing phase, features are extracted from the depth concatenation layer, which is further optimized using the Catch Fish Optimization (CFO) algorithm and passed to shallow wide neural network classifiers for the final prediction. In addition, the proposed DenseIncepS115 architecture is interpreted through Lime and Gradcam explainable techniques. Two publicly available datasets were employed in the experimental process: Alzheimer's ADNI and Alzheimer's classes MRI. On both datasets, the proposed architecture obtained an accuracy level of 99.5% and 98.5%, respectively. Detailed ablation studies and comparisons with state-of-the-art techniques show that the proposed architecture outperforms.
“Breaking barriers: empowering African youth through implementation of a therapeutic patient education program for juvenile idiopathic arthritis”
Authors: Hamdi Wafa , Migowa Angela , Lassoued Ferjani Hanene , Fazaa Alia , Faleye Ayodele , Miladi Saoussen , Maatallah Kaouther , Ben Abdelghani Kawther
Published: December 2024

Introduction

Patient education is crucial in managing chronic diseases like Juvenile Idiopathic Arthritis (JIA). Traditional methods such as videos and brochures often fail to maintain long-term knowledge retention. Therapeutic Patient Education (TPE) offers a more effective, patient-centered approach.

Objective

PAFLAR aimed to implement a TPE program with a “train the trainers” masterclass to ensure effective delivery and improve outcomes for children with JIA.

Methods

PAFLAR's TPE program was designed through a focus group, involving a literature review and strategic planning for the implementation step. The program includes specialized training for healthcare professionals enhancing their abilities to deliver TPE workshops. Participants were selected based on volunteerism and commitment to the project. Evaluations were conducted through feedback assessment of both patients and participants.

Results

PAFLAR launched a TPE program in 2023, aimed at training healthcare providers to implement TPE effectively. This initiative included both in-person and virtual masterclasses, resulting in five workshops conducted in Kenya, Tunisia, and Nigeria. These workshops covered various aspects of JIA management, such as patient and family education, self-esteem, physical activity, treatment adherence, and pain management. Early results showed significant improvements in patients’ and parents’ understanding and management of JIA, with positive feedback indicating a need for further sessions.

Conclusion

PAFLAR has taken the initial step in implementing a TPE program for JIA across three countries, representing different regions of Africa. The TPE program offers a promising alternative to traditional patient education methods, significantly improving patient care, empowering healthcare providers, and advancing healthcare systems.
Usenamine A: a potential therapeutic agent for rheumatoid arthritis and ankylosing spondylitis through its anti-inflammatory activity
Authors: Lee Yu Jeong , Li Zijun , Jang Hyun Hee , Kim Moon-Ju , Saravanakumar Kandasamy , Shim Seung Cheol , Cho Namki , Won Eun Jeong , Kim Tae-Jong
Published: December 2024

Background

Usenamine A (UA) is a natural compound isolated from the lichen Usnea diffracta, and its therapeutic effects on rheumatic diseases are not well understood. This study aimed to evaluate the potential anti-inflammatory effects of UA and its therapeutic effects on rheumatoid arthritis (RA) and ankylosing spondylitis (AS).

Materials and methods

Molecular docking was performed between the 3D structure of UA and the TNF-TNFR2 complex. Peripheral blood mononuclear cells (PBMCs) from RA and AS patients were treated with UA, and cell viability was measured using the MTS assay and flow cytometry. The in vitro effects of co-culture with UA were determined by measuring inflammatory cytokines, including IFN-γ, IL-17A, and GM-CSF, using flow cytometry and enzyme-linked immunosorbent assay (ELISA). The in vivo effects of UA were evaluated using an arthritis mouse model.

Results

The docking complex of UA bound to the TNF-TNFR2 complex exhibited docking scores of −5.251 kcal/mol and −6.274 kcal/mol, confirming their active sites. UA did not affect cell viability and suppressed the production of inflammatory cytokines in the PBMCs of RA (IFN-γ, IL-17A, and GM-CSF) and AS (GM-CSF) patients. The ELISA also confirmed reduced cytokine levels in the co-culture of UA and PBMCs from RA or AS patients. In the arthritis mouse model, significantly reduced clinical and histological scores were observed in the UA treatment group.

Conclusion

Our findings suggest that UA has potential as a binding target for TNF, suppresses inflammatory cytokines in PBMCs, and exhibits anti-inflammatory effects on arthritis in a mouse model.
Kidney outcomes associated with SGLT2 inhibitors compared to other glucose-lowering drugs: a real-world study from China
Authors: Xiao Xiang , Ji Shuming , Zheng Tao , Wang Tianzhu , Jiang Dapeng , Liu Fang
Published: December 2024

Objective

This study aimed to investigate the association between the utilization of Sodium-dependent glucose cotransporters inhibitors (SGLT2i) in real-world settings and kidney outcomes in patients with type 2 diabetes (T2D) and chronic kidney disease (CKD) in mainland China.

Methods

In a retrospective analysis of electronic medical records from West China Hospital of Sichuan University, patients with T2D and CKD were included. Patients were divided into two groups, those initiating treatment with SGLT2i and those receiving other glucose-lowering drugs (oGLDs). The primary focus lies in examining the impact of SGLT2i on the decline slope of eGFR and major kidney events in these patients.

Results

We enrolled 944 patients diagnosed with both T2D and CKD. Out of these, 605 patients were prescribed SGLT2i, while the remaining 339 patients received oGLDs. The median follow-up duration were 16.8 months and 20.6 months, respectively. Throughout the follow-up period, we observed a significant decrease in the rate of eGFR decline in patients using SGLT2i (4.94 mL/min/1.73 m2 per year reduction compared to oGLDs, 95% CI: 4.73–5.15). A total of 101 kidney composite endpoint events occurred, with 31 events in the SGLT2i group and 70 events in the oGLDs group. The use of SGLT2i was associated with a 65% decrease in the risk of kidney composite endpoint events (hazard ratio 0.35, 95% CI 0.19–0.63).

Conclusions

In clinical practice, SGLT2i have shown favorable effects on kidney prognosis in patients with T2D and CKD in mainland China. These effects remain consistent across patients with varying risks of CKD progression.

Clinical Trial Registration Number

ChiCTR2300068497.
A brief review of spin glass magnetometry techniques
Authors: Freedberg Jennifer , Dahlberg E. Dan
Published: December 2024

Spin glasses are inherently dynamical. Taken properly, measurements of these materials can capture their dynamics and provide a wealth of insight into the physics of the spin glass state. In this methods review, two magnetometry methods are directly compared–ac and dc. Because these measurements are taken differently, the resulting data of each method will contain different information about spin glass behavior. This review will specifically focus on how the out-of-equilibrium effects of aging, rejuvenation, and memory manifest in each of these techniques, and how to construct protocols to measure these effects. We then describe the physical significance of each type of measurement and how to interpret their results. Finally, we explicitly detail which applications are most appropriate for which method. This will help the reader select the most helpful technique to carry out their own future experiments.
Low-carbon optimal scheduling for distribution networks under supply and demand uncertainty
Authors: Nan Yu , Li Zhi , Gao Xin , Kou Xiaoshi
Published: December 2024

This paper presents a low-carbon optimal scheduling model for distribution networks with wind and photovoltaic (PV), accounting for supply and demand uncertainties. The model optimizes thermal generation costs, wind and PV maintenance costs, and carbon emissions using a chance-constrained approach with fuzzy variables. The clear equivalent class method simplifies these constraints for easier problem-solving. Validation on the IEEE-30 node system shows the model reduces costs by 32.9% and carbon emissions by 19.2% compared to traditional scheduling, effectively lowering both costs and the carbon footprint. This real-world optimization approach tackles uncertainty in renewable energy supply and improves system efficiency and sustainability.
Impact of exhaustive exercise on autonomic nervous system activity: insights from HRV analysis
Authors: Wang Weichao , Shao Mingrui , Du Weiping , Xu Yanjun
Published: December 2024

Introduction

Exhaustive exercise is a common training method in sports, but its impact on the autonomic nervous system of the human body remains unclear. Understanding the effects of exhaustive exercise on the body and its connection with the autonomic nervous system and central nervous system is crucial for guiding healthy training methods.

Methods

Twenty-three participants were selected, and exhaustive exercise intervention was performed using the Bruce Protocol. By measuring heart rate variability (HRV), the effects of exhaustive exercise on the autonomic nervous system function were analyzed.

Results

After exhaustive exercise, time-domain indices SDNN, RMSSD, and PNN50 all significantly decreased, with changes reaching significant levels (p < 0.01). Among them, the decrease in pNN50 was particularly pronounced, with a change rate of −94.55%. Frequency-domain indices VLF, LF, and HF also showed significant decreases (p < 0.01), but the ratio of LF to HF showed an upward trend (p < 0.01), with LF showing a greater decrease. Nonlinear indices SD1 and SD2 showed extremely significant decreases (p < 0.01), and the SD2/SD1 ratio showed a significant increase (p < 0.01), indicating significant changes in HRV nonlinear characteristics after exercise.

Discussion

Exhaustive exercise leads to a decrease in autonomic nervous system activity and an increase in sympathetic nervous system activity. These findings underscore the profound impact of exhaustive exercise on the autonomic nervous system, with implications for understanding the physiological responses to intense physical exertion. Further research is warranted to explore the long-term effects of exhaustive exercise on autonomic regulation and its potential implications for training methodologies and athlete health.
Insights into the functional mechanisms of three terpene synthases from Lavandula angustifolia (Lavender)
Authors: Liu Dafeng , Song Hongying , Deng Huashui , Abdiriyim Ablikim , Zhang Lvxia , Jiao Ziwei , Li Xueru , Liu Lu , Bai Shuangqin
Published: December 2024

Lavender species are of significant economic value being cultivated extensively worldwide for their essential oils (EOs), which include terpenes that play crucial roles in the cosmetic, personal care, and pharmaceutical industries. The terpene synthases in lavender, such as Lavandula angustifolia linalool synthase (LaLINS), limonene synthase (LaLIMS), and bergamotene synthase (LaBERS), are key enzymes in terpene biosynthesis. However, the functional mechanisms underlying these enzymes remain poorly understood. Here, we used AlphaFold2 to predict the three-dimensional structures of LaLINS, LaLIMS, and LaBERS. The hydrodynamic radii of LaLINS, LaLIMS, and LaBERS were 5.7 ± 0.2, 6.2 ± 0.3, and 5.4 ± 0.2 nm, respectively. Mutations D320A or D324A led to a complete loss of activity in LaLINS compared to the wild-type (WT) enzyme; similarly, mutations D356A or D360A abolished activity in LaLIMS, and D291A or D295A eliminated activity in LaBERS. Furthermore, the genes LaLINS, LaLIMS, and LaBERS exhibited significantly higher expression levels in leaves compared to stems and flowers, with peak expression occurring at 8:00 a.m. Our findings contribute to a deeper understanding of terpene biosynthesis in lavender and offer insights for improving essential oil production through genetic engineering.
A randomized trial investigating the impact of response expectancy on the counting blessings intervention: the role of optimism as a moderator
Authors: Predatu Petronela , David Daniel , Kirsch Irving , Florean Ionuț Stelian , Predatu Răzvan
Published: December 2024

Introduction

This randomized controlled trial aimed to address a knowledge gap concerning the mechanisms responsible for the efficacy of gratitude interventions. Specifically, we investigated how various response expectancies (positive, ambiguous + negative, and no expectancy) impact the efficacy of the “counting blessings” intervention in influencing positive and negative emotions. Additionally, the study explores how optimism levels (high, medium, low) interact with these expectancies to influence intervention efficacy.

Method

A total of 529 adult volunteers were recruited through social media and randomly assigned to one of three experimental conditions, Positive Condition (PC), Ambiguous + Negative Condition (ANC), No Expectancy Condition (NEC), using a Random Sequence Generator. Of these, 142 participants completed the seven-day counting blessings intervention, and 111 participated in a follow-up assessment 1 month later. Missing data were addressed using multiple imputation. The main outcomes were changes in positive and negative emotions, with moderation analysis assessing the interaction between optimism levels and response expectancies. The study adhered to the CONSORT guidelines.

Results

While no significant interaction was found between experimental conditions and time regarding emotional outcomes (p ˃ 0.05), moderation analysis revealed differential interactions between optimism levels and expectancies, particularly influencing positive emotions (p < 0.009). For participants with low optimism, positive emotions significantly increased from post-intervention to follow-up in the PC (t = −2.42, p < 0.016) and from pre-intervention to post-intervention in the ANC (t = 2.41, p < 0.018). Participants with medium optimism experienced an increase in positive emotions across all conditions from pre-intervention to follow-up and from post-intervention to follow-up (ps < 0.05). High optimism participants showed an increase in positive emotions from pre-intervention to follow-up and post-intervention to follow-up in the PC (t = 2.09, p < 0.038 and t = 3.06, p < 0.003) and NEC c (t = −2.76, p < 0.006 and t = 2.74, p < 0.007).

Conclusion

Our findings emphasize the effectiveness of a brief gratitude journal and underscore the nuanced role of response expectancy, especially in interaction with the initial level of optimism, in enhancing positive emotions. These results hold significance for both theoretical understanding and clinical applications.
Stroke and liminality: narratives of reconfiguring identity after stroke and their implications for person-centred stroke care
Authors: Hall Joseph , van Wijck Frederike , Kroll Thilo , Bassil-Morozow Helena
Published: December 2024

Background

The complex physical, cognitive, and psychological consequences of stroke can disrupt a survivor's sense of pre-stroke normality and identity. This can have a substantial impact on their individual and social lives. Individual reports about life after stroke have improved our understanding of this impact. However, stroke support systems, struggling with increased demands due to a growing stroke population and guideline requirements, require deeper insights based on synthesised narratives into what can enable stroke survivors to rebuild their lives and identities positively to provide person-centred care.

Methods

A qualitative study using Charmaz's Constructivist Grounded Theory (GT) method. Semi-structured interviews lasting 60–90 min were conducted. These interviews were held at least 12 months post-stroke.

Findings

Thirty participants were interviewed from across the UK (14 women, 16 men; aged 31–86; 1–25 years post-stroke). Participants reported the disruption stroke could cause to their sense of identity. The concept of liminality, that describes the ambiguous, transformative state between two distinct stages, where an individual or group exists “betwixt and between” stable conditions, explains the challenge to identity post-stroke. Participants reported developing an uncertain sense of identity as they struggled to structure identity in the same way they did before stroke. This is because the participants' characteristics, traits, hobbies, or future life plans, as well as social relationships and roles, were affected by stroke. Subsequently, participants began a process of reconfiguring their identity, an often-long-term process that involved coming to terms with, and integrating, the impact of stroke on their lives. As a result, participants could enter an indefinite period of sustained liminality as they contend with long-term change and continued uncertainty.

Conclusion

The concept of liminality, which emerged from individual stroke narratives for the first time, conveyed the adaptive and enduring nature of a stroke survivor's journey. Post-stroke liminality may continue indefinitely, sustained by a survivor's subjective individual and social situation. This new insight justifies the urgent call for long-term rehabilitation and support that is tailored towards the unique nature of a survivor's circumstances. Further work is required to understand how tailored, long-term and person-centred support can encourage survivors to positively reconfigure their identity.