bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Epoxidized Natural Rubber of the thermoformability of Poly(Lactic Acid) biopolymer films using elevated temperature ball burst tests
Authors: Tábi T. , Gere D. , Csézi G. , Pölöskei K.
Published: November 2023

In this paper, we investigated the thermoformability of Epoxidized Natural Rubber (ENR)-filled Poly(Lactic Acid) (PLA) films using elevated temperature tensile and ball burst tests. Three different d-Lactide contents of PLA were used to be able to analyze the effect of crystallization and crystallinity. We observed that the ENR acted as a nucleating agent in PLA when the blend was cooled from melt or cold crystallized, but it retarded the drawing-induced crystallization of PLA at elevated temperature tensile testing since ENR droplets acted as obstacles. The effect of NR on PLA crystallization was also found to be dependent on d-Lactide content. In the ball burst test, different behaviors were observed for PLA with the lowest d-Lactide content, which was caused by the complex stress state that even more induced crystallization of PLA and then a single-axis tensile test.
Epistemic Modality Constructions as Stable Idiolectal Features: A Cross-genre Study of Spanish
Authors: Mojedano Batel Andrea , Soler Bonafont Amparo , Kredens Krzysztof
Published: November 2023

Forensic authorship analysis is based on two assumptions: that every individual has a unique idiolect, and that features characteristic of that idiolect will recur with a relatively stable frequency. Yet, a speaker’s language can change with age, affective states, according to audience, or genre. Thus, studies on authorship analysis should adopt the theory that while some linguistic parameters of an idiolect can remain stable, others can change depending on various circumstances. This investigation, which takes a constructional and functional-based approach to discourse-level phenomena in idiolectal stability, analyzes cross-genre data produced by nine Mexican participants throughout a twelve-year time span. This study contributes to a greater understanding of the linguistic elements that survive genre effects and are potentially useful in both investigative and evidential forensic linguistic work. We provide a detailed description of linguistic features, their specific values, and context-dependent interpretation, keeping in mind the context of expert linguistic testimony, with its preference for methods which “employ linguistically motivated analyses in combination with quantitative tools” (Solan & Tiersma, 2004, p.463).

Our findings show that idiolectal style tends to remain stable across genres and communication modes in epistemic modality constructions. Epistemic markers —specifically, markers indicating low commitment by the speaker (e.g., no sé ‘I don’t know’) or expressing indirectness when introducing the illocutionary force (e.g., la verdad [es que] ‘the truth [is that]’)— display idiolectal stability, as these markers seem to be the most effective in terms of allowing speakers to strategically manifest the extent of their knowledge regarding what is said.
Bataille and the Poverty of Academic Form
Authors: Allen Ansgar
Published: November 2023

This paper argues that the dominant modes of academic address, the conference paper, the journal article, and the monograph, reinforce problematic and exclusionary assumptions concerning what counts as legitimate research, whilst also restricting academic enquiry and impoverishing intellectual life. It makes its case by exploring in some detail the intellectual commitments of one the West’s more wayward 20th century thinkers, Georges Bataille. It suggests that Bataille presents not simply a conceptual armoury (and one among many) for critiquing Western logocentrism from within, but offers an example of what a less domesticated, less stylistically narrowed mode of thinking might look like.
The effect of gunshot detection technology on evidence collection and case clearance in Kansas City, Missouri
Authors: Piza Eric L. , Arietti Rachael A. , Carter Jeremy G. , Mohler George O.
Published: November 2023

Objectives

This study tests whether (1) shots fired calls for service in the gunshot detection technology (GDT) target area are more likely to be classified as unfounded; (2) police responses to shootings in the GDT target area are more likely to recover ballistic evidence or firearms; and (3) shootings in the GDT target area are more likely to be cleared.

Methods

Entropy balancing created a weighted control group that equaled the treatment group across a range of covariates. GDT effect was tested through logistic regression models with entropy balancing weights set as probability weights.

Results

Shots fired occurring in the GDT target area were 15% more likely to be classified as unfounded compared to control cases. GDT did not significantly influence the likelihood of evidence collection or case clearance in shooting incidents.

Conclusions

GDT may not add investigative value to police responses to shooting incidents and may increase patrol workload.
Slicing the Nash equilibrium manifold
Authors: Levy Yehuda John
Published: November 2023

This paper uses tools on the structure of the Nash equilibrium correspondence of strategic-form games to characterize a class of fixed-point correspondences, that is, correspondences assigning, for a given parametrized function, the fixed-points associated with each value of the parameter. After generalizing recent results from the game-theoretic literature, we deduce that every fixed-point correspondence associated with a semi-algebraic function is the projection of a Nash equilibrium correspondence, and hence its graph is a slice of a projection, as well as a projection of a slice, of a manifold that is homeomorphic, even isotopic, to a Euclidean space. As a result, we derive an illustrative proof of Browder’s theorem for fixed-point correspondences.
DLK-MAPK Signaling Coupled with DNA Damage Promotes Intrinsic Neurotoxicity Associated with Non-Mutated Tau
Authors: Li Sanming , Roy Ethan R. , Wang Yanyu , Watkins Trent , Cao Wei
Published: November 2023

Alzheimer’s disease (AD) is the most prevalent form of neurodegeneration. Despite the well-established link between tau aggregation and clinical progression, the major pathways driven by this protein to intrinsically damage neurons are incompletely understood. To model AD-relevant neurodegeneration driven by tau, we overexpressed non-mutated human tau in primary mouse neurons and observed substantial axonal degeneration and cell death, a process accompanied by activated caspase 3. Mechanistically, we detected deformation of the nuclear envelope and increased DNA damage response in tau-expressing neurons. Gene profiling analysis further revealed significant alterations in the mitogen-activated protein kinase (MAPK) pathway; moreover, inhibitors of dual leucine zipper kinase (DLK) and c-Jun N-terminal kinase (JNK) were effective in alleviating wild-type human tau-induced neurodegeneration. In contrast, mutant P301L human tau was less toxic to neurons, despite causing comparable DNA damage. Axonal DLK activation induced by wild-type tau potentiated the impact of DNA damage response, resulting in overt neurotoxicity. In summary, we have established a cellular tauopathy model highly relevant to AD and identified a functional synergy between the DLK-MAPK axis and DNA damage response in the neuronal degenerative process.
Electroacupuncture Exerts Analgesic Effects by Restoring Hyperactivity via Cannabinoid Type 1 Receptors in the Anterior Cingulate Cortex in Chronic Inflammatory Pain
Authors: Wu Junshang , Hua Libo , Liu Wenhao , Yang Xiaoyun , Tang Xiaorong , Yuan Si , Zhou Sheng , Ye Qiuping , Cui Shuai , Wu Zhennan , Lai Lanfeng , Tang Chunzhi , Wang Lin , Yi Wei , Yao Lulu , Xu Nenggui
Published: November 2023

As one of the commonly used therapies for pain-related diseases in clinical practice, electroacupuncture (EA) has been proven to be effective. In chronic pain, neurons in the anterior cingulate cortex (ACC) have been reported to be hyperactive, while the mechanism by which cannabinoid type 1 receptors (CB1Rs) in the ACC are involved in EA-mediated analgesic mechanisms remains to be elucidated. In this study, we investigated the potential central mechanism of EA analgesia. A combination of techniques was used to detect the expression and function of CB1R, including quantitative real-time PCR (q-PCR), western blot (WB), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and in vivo multichannel optical fibre recording, and neuronal activity was examined by in vivo two-photon imaging and in vivo electrophysiological recording. We found that the hyperactivity of pyramidal neurons in the ACC during chronic inflammatory pain is associated with impairment of the endocannabinoid system. EA at the Zusanli acupoint (ST36) can reduce the hyperactivity of pyramidal neurons and exert analgesic effects by increasing the endocannabinoid ligands anandamide (AEA), 2-arachidonoylglycerol (2-AG) and CB1R. More importantly, CB1R in the ACC is one of the necessary conditions for the EA-mediated analgesia effect, which may be related to the negative regulation of the N-methyl-D-aspartate receptor (NMDAR) by the activation of CB1R downregulating NR1 subunits of NMDAR (NR1) via histidine triad nucleotide-binding protein 1 (HINT1). Our study suggested that the endocannabinoid system in the ACC plays an important role in acupuncture analgesia and provides evidence for a central mechanism of EA-mediated analgesia.
Pilot Test of Mopati, a Multi-Level Adherence Intervention for People Living with HIV and Their Treatment Partners in Botswana
Authors: Bogart Laura M. , Phaladze Nthabiseng , Kgotlaetsile Keonayang , Klein David J. , Goggin Kathy , Mosepele Mosepele
Published: November 2023

Background

Low-cost, scalable strategies are necessary to reach the UNAIDS 2030 target of ending HIV as a public health threat. Use of treatment partners, informal caregivers selected by people living with HIV to support antiretroviral therapy adherence, is one such strategy that is included in many countries’ HIV guidelines, including Botswana, a country with high HIV prevalence.

Method

From June 2021 to June 2022, we pilot tested a clinic-based treatment partner intervention (“Mopati”), including standardized language for providers to guide patients on treatment partner selection and workshops to train treatment partners on providing non-directive support to patients using a non-confrontational, non-judgmental approach. Sixty unsuppressed patients (30 per clinic) and 45 treatment partners (17 intervention, 28 control) were recruited from an intervention–control clinic matched-pair in Gaborone, Botswana.

Results

Mopati had medium-to-large effects on increasing patients’ adherence, adherence self-efficacy, intrinsic adherence motivation, and perceived non-directive support from treatment partners, and decreasing treatment partner caregiver burden. Aggregate viral suppression rates significantly increased in the intervention (vs. control) clinic. Qualitative data from 14 clinic staff, 21 patients, and 16 treatment partners indicated that Mopati was viewed as effective. Providers said the guidance empowered them to be proactive in communicating about adherence; most reported using the guidance.

Conclusion

This study shows preliminary support for the use of treatment partners in HIV care, and further evidence for interventions that leverage patients’ existing support. This research can inform ways to improve adherence to HIV treatment as well as the treatment of HIV-related comorbid conditions in lower-resource settings.

Trial Registration

ClinicalTrials.gov Identifier: NCT04796610.
Ethical Issues in Photovoice Studies involving Key Populations: A Scoping Review
Authors: Ng Chong Guan , Ting Sing Qin , Saifi Rumana Akhter , Kamarulzaman Adeeba Bt
Published: November 2023

Photovoice, a community-based participatory research method, employs images and words to convey participants' needs, concerns, and desires. It proves particularly valuable in researching marginalized communities who face elevated health risks, disease transmission, and social and health disparities. This paper seeks to investigate the ethical considerations inherent in photovoice research projects. We conducted an extensive literature review spanning four databases to identify pertinent photovoice studies. Ethical issues from the selected articles were identified, categorized, and summarized. Our analysis of twenty-five photovoice studies uncovered various ethical concerns, which had been grouped into informed consent, participant safety and disclosure, privacy and confidentiality, misrepresentation, power dynamics, and compensation. In essence, our findings underscore the importance of addressing these ethical concerns to uphold the rights and autonomy of participants, even as photovoice research strives for authenticity, inclusivity, and empowerment.
Representations of Ecuadorian cuisine in the coast and the highlands regions through the free listing technique
Authors: Rojas-Le-Fort Marlene , Valdivieso-López Isabel Patricia , Duarte-Casar Rodrigo
Published: November 2023

Codification of country-of-origin cuisines serves as a powerful tool for the international promotion of a country and the reinforcement of its national identity. Even though there have been efforts in that direction, Ecuadorian gastronomy is not consensually codified. This study aims to compare the perceptions of Ecuadorian gastronomy among inhabitants of coastal and highland cities —where most of the population of the country lives— using free listing as a study technique, to distinguish consumers’ demographics through their representations, to define a list of the most culturally salient preparations, and to compare these perceptions with the traditional Ecuadorian dishes set forth by the Ministry of Tourism for the promotion of Ecuadorian cuisine. Data were acquired through face-to-face surveys in the main coastal and highland cities of Ecuador (n = 294) in which participants were asked to list the main dishes of Ecuadorian cuisine. The study identifies 25 preparations that exhibit the highest cultural consensus as determined by B'score. The four most salient dishes areencebollado(tuna, onion, and cassava soup),ceviche(lime-cured fish or shrimp cold soup),hornado, andfritada(roast and fried pork dishes, respectively). The representations of The Coast and Highlands show weak correlation (0.286 in Spearman’s test) despite having 60% consensus in the most salient dishes, and other groups show much higher consensus. There is agreement with most provincial dishes selected for the promotion programs of Ecuadorian cuisine, although there is room for improvement. This study is valuable to help codify Ecuadorian gastronomy from a grassroots perspective, to point out promotion and local development practical implications for both domestic and international tourism, and to lay the groundwork for further research.
The Impact of Social Media on the Physical and Mental Well-Being of Medical Students During the COVID-19 Pandemic
Authors: Mahevish Raaina , Khan Aisha , Mahmood Hareem Rashid , Qazi Sadia , Fakhoury Hana M. A. , Tamim Hani
Published: November 2023

COVID-19 pandemic has increased social media engagement globally. This study examined the correlation between social media use and physical/mental health among university students, considering gender and academic year. Out of 146 responses, 119 were analyzed after excluding participants with pre-existing psychological conditions. Results showed a significant correlation between social media use and mental health for all participants (correlation coefficient = 0.30, p < 0.001), indicating a negative impact on mental health with increased use. Gender-specific analysis revealed a non-significant correlation among males (p = 0.21), while females exhibited a significant correlation (correlation coefficient = 0.32, p = 0.01), suggesting an adverse effect on their mental health. Regarding physical health, females displayed an even higher correlation (correlation coefficient = 0.40, p < 0.001), highlighting the negative influence of social media on their physical well-being. Conversely, no significant correlation was observed among males. Analyzing by academic year, both pre-clerkship and clerkship students showed a significant correlation between social media use and mental health (correlation coefficients of 0.26, p = 0.01, and 0.42, p = 0.03, respectively). Similarly, a significant correlation was found between social media use and physical health among pre-clerkship students (correlation coefficient = 0.34, p = 0.001), but not among clerkship students. In conclusion, this study provides evidence of the adverse impact of social media use on physical and mental health among university students, particularly among females and across different academic years. These findings underscore the importance of promoting healthy social media habits and raising awareness about the potential negative effects on well-being.
Measuring the level of rurality in the Southwestern region of Bangladesh
Authors: Rahman Md. Abdur , Hossain Md. Zakir , Ha-Mim Nur Mohammad , Tanvir Farhan , Islam Sazzadul , Rahaman Khan Rubayet
Published: November 2023

This research intends to measure and characterize the level of rurality in the southwestern region of Bangladesh using an indexing approach from functional perspective. The findings of the study can be conducive for efficient decision making related to rural development policies and planning. Besides, operational delineation of administrative units, such as Unions and Wards, is essential for the formulation and effective implementation of evidence-based development initiatives. The study has utilized the latest available population census data published by Bangladesh Bureau of Statistics (BBS). The results reveal that Satkhira district exhibits a higher degree of rurality (RI = 4.23) compared to Khulna (RI = 3.58) and Bagerhat (RI = 4.14) districts. This variation among the three districts is ascribed to existing rural–urban disparities. The study underscores the primary influence of socio-economic factors on the rural–urban disparities, with a secondary role for demographic and infrastructural aspects. However, education's impact was notably limited in this context. Insights on intra-district disparities infer that Khulna district exhibits significantly greater rural–urban disparities than the other two. The study also highlights the clustering of non-rural areas along major rivers. This research will assist rural planners and policy makers in understanding the specific rural dynamics of the study area, essential for tailoring fit-to-context development strategies. Moreover, the study provides a basis for classifying the region into clusters to streamline development priorities and resource allocation. Furthermore, the article presents a transferable methodology for evaluating rurality and delineating rural regions in different contexts.
The Computational Theory of Mind
Authors: Colombo Matteo , Piccinini Gualtiero
Published: November 2023

The Computational Theory of Mind says that the mind is a computing system. It has a long history going back to the idea that thought is a kind of computation. Its modern incarnation relies on analogies with contemporary computing technology and the use of computational models. It comes in many versions, some more plausible than others. This Element supports the theory primarily by its contribution to solving the mind-body problem, its ability to explain mental phenomena, and the success of computational modelling and artificial intelligence. To be turned into an adequate theory, it needs to be made compatible with the tractability of cognition, the situatedness and dynamical aspects of the mind, the way the brain works, intentionality, and consciousness.
Les savoirs de l’expérience
Authors: Grancher Romain
Published: November 2023

Fondé sur l’analyse d’une trentaine d’expériences de pêche réalisées sur les côtes de la Manche entre les années 1680 et 1860, cet article décrit les premières manifestations d’un mode de gouvernement expérimental des ressources de la mer sous l’Ancien Régime, puis sa normalisation progressive au cours du xixe siècle. Il montre comment les mesures de police des pêches destinées à assurer la conservation de ces ressources sont le résultat d’une négociation entre l’administration centrale de la marine et les communautés de pêcheurs, dont le monopole sur la production du savoir halieutique leur permet de défendre leur particularisme technique et juridique.
The Pandemic’s Golden Touch: (Neo)Extractivism, Coloniality, and Necropolitics on Brazil’s Indigenous Territories
Authors: Macias Gimenez Rebeca B.
Published: November 2023

Mining has been at the forefront of coloniality for hundreds of years in Brazil, representing one of the main threats to the integrity and health of Indigenous lands. The 1988 Brazilian Constitution recognized Indigenous peoples’ rights to the lands they occupy, and their natural resources, according to their traditions, uses, beliefs, and practices. Constitutional provisions, however, have not impeded governments and lawmakers from actively enabling extractive activities in Indigenous territories and their surroundings. Recently, the Bolsonaro government proposed a package of laws and policies to legalize mineral exploitation on Indigenous lands, using the economic uncertainties generated by the COVID-19 pandemic as a justification. However, this action must be explained through the paradigms (or philosophical frameworks) of the extractive economy and coloniality of power, operationalized by necropolitics. The article’s main argument is that the Constitution requires the government to engage in practices of decoloniality that express Indigenous legal traditions. Even though a newly elected government has been revoking many of Bolsonaro’s proposals, the paradigms of the extractive economy and the coloniality of power have a profound, structural influence on the Brazilian legal and political systems and must be challenged by a revival of decolonial ways of thinking and acting.
Smooth models for certain fibered partially hyperbolic systems
Authors: DOUCETTE MEG
Published: November 2023

We prove that under restrictions on the fiber, any fibered partially hyperbolic system over a nilmanifold is leaf conjugate to a smooth model that is isometric on the fibers and descends to a hyperbolic nilmanifold automorphism on the base. One ingredient is a result of independent interest generalizing a result of Hiraide: an Anosov homeomorphism of a nilmanifold is topologically conjugate to a hyperbolic nilmanifold automorphism.
Gambling and Elizabethan Gentlemen
Authors: Ball Patrick Seymour
Published: November 2023

Before the mid-seventeenth century when a developing understanding of probability transformed gambling, English gaming took place in the community rather than in dedicated institutions like casinos and so represented and interacted with more general social behavior. Different communities gambled differently; they had different status under the law. This article considers gentlemen's gambling, arguing that in the absence of other constraints, notions of honor had a key role in shaping that activity. Contemporary accounts such as Sir John Harington's “Treatise on Playe” suggest that high-stakes wagering fell into the anthropological category of deep play, whereby gamesters staked excessive sums to win renown for their daring; secondly, it appears that such behavior was seen as a young man's activity, with older men condemning immoderate wagering as their ideas about what was honorable shifted as they matured and became integrated into the community. In addition to age-related changes of attitude to gambling, a tension existed between Elizabethan ideals of gentlemen's gambling behaviors and individual gamesters’ real circumstances. Some had limited money for wagering, others little time; youths from gentle families were sometimes indentured as apprentices or otherwise in situations that altered their relationships to time, money, and regulation. Consequently, even within this single sector of Elizabethan society, attitudes to gambling acquired a high level of complexity.
Mercy and the Construction of Social Control: A Four-Site Analysis of Clemency
Authors: Horowitz Veronica L.
Published: November 2023

Executive clemency is a discretionary power to grant legal mercy and is rarely given. Using data from contemporary clemency hearings in four states, I explore how state actors use commutation hearings as sites to exert social control and communicate hegemonic ideology. Clemency board members use hearings as sites to communicate the behaviors and attitudes they desire from applicants, aiming to generate compliance and consent. These state actors use the ritual of the clemency process and the hope afforded by the possibility of legal mercy as tools to motivate desired behaviors and beliefs among those in prison both explicitly and implicitly in these hearings. Such behaviors and beliefs, accordingly, are those that serve to reinforce the control and power of the state.
The difference multiplicity makes: The American Civil War as passive revolution
Authors: Anievas Alexander , Waring Dabney
Published: November 2023

This article examines and further develops the relationship between the theory of uneven and combined development (UCD), recently taken up by International Relations (IR) scholars to furnish a social theory of ‘the international’, and the Gramscian concept of ‘passive revolution’, which refers to a molecular process of top-down revolution and state formation that preserves ruling-class power by transforming its social base. To this end, the paper: (1) advances a productive distinction between ‘societal’ and ‘(geo)political’ multiplicity, increasing the transdisciplinary potential of UCD and challenging dominant state-centric approaches to IR; (2) demonstrates that UCD is central to creating the conditions for passive revolution; and, (3) argues that UCD illuminates the distinct spatial dimensions of passive revolution, for which the succession of ‘classes’ in time requires the expansion of capitalist social relations in space. To illustrate these claims, the article demonstrates how the American Civil War is best understood as an inter-societal conflict, exacerbated by the coexistence of two social formations within a single state, leading to war. It then shows how, upon victory, the North’s abolition of enslaved labour and the subsequent attempt to re-subsume the South within a single sovereign polity constituted a radical instance of passive revolution.
Compassion fatigue and psychological resilience levels of nursing final students: A descriptive, cross-sectional, and relational study
Authors: Karabey Tuba
Published: November 2023

Objectives

Nursing students often encounter real-life trauma situations during their clinical experience and similar workplace environment and climate as professional nurses. This research was carried out to determine the reflections of the compassion fatigue levels of nursing final year students on their psychological resilience levels.

Methods

This research was conducted as descriptive and cross-sectional. The sample of the study consists of 250 final year nursing students. Data were collected using Personal Information Form, Compassion Fatigue Scale, and Resilience Scale. It was determined that the compassion fatigue and psychological resilience levels of nursing final year students were moderate.

Results

It was determined that as the levels of compassion fatigue increased, psychological resilience decreased. A highly negative and significant correlation was found between the total and sub-dimension mean scores of compassion fatigue and psychological resilience (p = 0.001).

Significance of results

It was found that there was a negative and high level relationship between nursing students’ compassion fatigue levels and all its sub-dimensions and psychological resilience. Resilience can improve psychosocial functioning and professional performance, including for nursing students facing stressful clinical experiences. It is important to determine the compassion fatigue and psychological resilience levels of nursing students, to determine the existing problems, and to take initiatives for this.
ONTOLOGICAL PURITY FOR FORMAL PROOFS
Authors: MARTINOT ROBIN
Published: November 2023

Purity is known as an ideal of proof that restricts a proof to notions belonging to the ‘content’ of the theorem. In this paper, our main interest is to develop a conception of purity for formal (natural deduction) proofs. We develop two new notions of purity: one based on an ontological notion of the content of a theorem, and one based on the notions of surrogate ontological content and structural content. From there, we characterize which (classical) first-order natural deduction proofs of a mathematical theorem are pure. Formal proofs that refer to the ontological content of a theorem will be called ‘fully ontologically pure’. Formal proofs that refer to a surrogate ontological content of a theorem will be called ‘secondarily ontologically pure’, because they preserve the structural content of a theorem. We will use interpretations between theories to develop a proof-theoretic criterion that guarantees secondary ontological purity for formal proofs.
Spatiotemporal Evolution of Riparian Redox Zonation in Response to River Stage Fluctuation and Dynamic Biofilm Growth
Authors: Zhu Yonghui , Dai Heng , Wen Zhang , Liu Hui , Yuan Songhu
Published: November 2023

Riparian zones are one of the most complex redox‐dynamic systems, where sequential redox zones of dissolved oxygen, nitrate, manganese dioxide, ferric hydroxide and sulfate reduction commonly form. River stage dynamics and microbes are two key controls on nutrient transformation in the riparian zone. Microbial growth, on one hand, increases the biogeochemical reaction rate by altering the microbial population. On the other hand, decreases the permeability of the media due to clogging effects, thereby increasing the residence time of solutes. However, previous studies have often concentrated on steady‐state flow systems and overlooked the impacts of river stage fluctuations and dynamic microbial growth on redox zonation. In this study, we investigated the interactions among river fluctuations, nutrient supplies, microbial metabolisms, and sediment physical properties in a dynamic riparian system. A column experiment was conducted, and a one‐dimensional modeling framework that coupled flow, reactive solute transport, dynamic microbial growth and bioclogging processes was developed to study the spatiotemporal evolution of redox zonation in response to river stage fluctuations and microbial growth. Our results showed that two redox zonation patterns, including a typical sequence and an interlaced sequence, could form in the dynamic riparian zone. Increasing exogenous dissolved organic carbon concentration facilitates the occurrence of anomalous sequence redox zonation. Modeling results also indicated that ignoring microbial growth leads to a significant difference in the spatiotemporal characterization of the redox zonation, especially for a more permeable sediment. Our results implicate the occurrence of more complicated redox zonation in a dynamic riparian system than previously reported.
Differentiating EDRs From the Background Magnetopause Current Sheet: A Statistical Study
Authors: Beedle J. M. H. , Gershman D. J. , Uritsky V. M. , Phan T. D. , Giles B. L.
Published: November 2023

The solar wind is a continuous outflow of charged particles from the Sun's atmosphere into the solar system. At Earth, the solar wind's outward pressure is balanced by the Earth's magnetic field in a boundary layer known as the magnetopause. Plasma density and temperature differences across the boundary layer generate the Chapman‐Ferraro current which supports the magnetopause. Along the dayside magnetopause, magnetic reconnection can occur in electron diffusion regions (EDRs) embedded into the larger ion diffusion regions (IDRs). These diffusion regions form when opposing magnetic field lines in the solar wind and Earth's magnetic field merge, releasing magnetic energy into the surrounding plasma. While previous studies have given us a general understanding of the structure of the diffusion regions, we still do not have a good grasp of how they are statistically differentiated from the non‐diffusion region magnetopause. By investigating 251 magnetopause crossings from NASA's Magnetospheric Multiscale (MMS) Mission, we demonstrate that EDR magnetopause crossings show current densities an order of magnitude higher than regular magnetopause crossings—crossings that either passed through the reconnection exhausts or through the non‐reconnecting magnetopause, providing a baseline for the magnetopause current sheet under a wide range of driving conditions. Significant current signatures parallel to the local magnetic field in EDR crossings are also identified, which is in contrast to the dominantly perpendicular current found in the regular magnetopause. Additionally, we show that the ion velocity along the magnetopause is highly correlated with a crossing's location, indicating the presence of magnetosheath flows inside the magnetopause.
Increased DIN Storage and ΔDIC/ΔDIN Ratio in the Subsurface Water of the Canada Basin, 1990‒2015
Authors: Zhang Tianzhen , Hao Qiang , Jin Haiyan , Bai Youcheng , Zhuang Yanpei , Qi Di , Chen Jianfang
Published: November 2023

The subsurface layer of the Canada Basin contains large reservoirs of dissolved inorganic nitrogen and carbon (DIN and DIC, respectively). Under rapid change of Arctic climate system, these reservoirs may not only serve as potential material bases for primary production, but also for carbon source and sink. However, the long‐term trends in reservoirs and the interaction between reservoirs and the upper ocean are not fully understood. This study used two data sets to evaluate the long‐term trends in the DIN and DIC reservoirs in the subsurface layer of the Canada Basin during 1990–2015. The results indicate an increase of ∼35% in the subsurface DIN reservoir over 25 years, whereas the DIN concentration remained fairly constant. The discrepancy between the DIN reservoir and DIN concentration was primarily owing to the expansion of the subsurface layer, which increased the DIN stock but maintained a relatively stable DIN concentration. Additionally, the ΔDIC/ΔDIN ratio in the subsurface layer increased by ∼33% over 25 years, primarily due to denitrification on the pathway of Pacific inflow, such as Chukchi shelf. In the Canada Basin, the enhanced mixing between the subsurface and surface layers may promote primary production and render the subsurface layer a carbon source. Consequently, when studying the biogeochemical cycles in the changing Arctic Ocean, the long‐term interactions between the subsurface and surface layers in the Canada Basin should be further considered.
Enhanced Phosphate Consumption Stimulated by Nitrogen Fixation Within a Cyclonic Eddy in the Northwest Pacific
Authors: Yuan Zhongwei , Browning Thomas J. , Du Chuanjun , Shen Hui , Wang Lei , Ma Yifan , Jiang Zong‐Pei , Liu Zhiyu , Zhou Kuanbo , Kao Shuh‐Ji , Dai Minhan
Published: November 2023

Mesoscale eddies are common in the subtropical Northwest Pacific, however, relatively little is known about their spatial variability and temporal evolution, and how these impact upper ocean biogeochemistry. Here we investigate these using observations of a cyclonic eddy carried out along four sequential transects. Consistent with previous observations of cyclonic eddies, the eddy core had doming isopycnals, bringing elevated nutrient waters nearer to the surface. However, we also found that the upper layer of the eddy above the nutricline had significantly lower phosphate concentrations within its core relative to its edge. We attributed this to elevated N2 fixation within the eddy core, which was likely driven by enhanced subsurface iron supply, ultimately resulting in increased phosphate consumption. Eddy‐enhanced N2 fixation was additionally supported by the elevation of nitrate + nitrite to phosphate ratios below the euphotic zone. Moreover, we observed that while the upward displacement of isopycnals within the eddy core led to an increase in phytoplankton biomass in the lower euphotic zone, there was no significant increase in total phytoplankton biomass across the entire euphotic zone. Cyclonic eddies in the subtropical North Pacific are projected to be becoming more frequent, implying that such dynamics could become increasingly important for regulating nutrient biogeochemistry and ultimately productivity of the region.
Communication Distance and Bayesian Inference in Non‐Perennial Streams
Authors: Aho Ken , Derryberry Dewayne , Godsey Sarah E. , Ramos Rob , Warix Sara R. , Zipper Samuel
Published: November 2023

Non‐perennial streams are receiving increased attention from researchers, however, suitable methods for measuring their hydrologic connectivity remain scarce. To address this deficiency, we developed Bayesian statistical approaches for measuring both average active stream length, and a new metric called average communication distance. Average communication distance is a theoretical increasedeffective distancethat stream‐borne materials must travel, given non‐continuous streamflow. Because it is the product of the inverse probability of surface water presence and stream length, the average communication distance of a non‐perennial stream segment will be greater than its actual physical length. As an application we considered Murphy Creek, a simple non‐perennial stream network in southwestern Idaho, USA. We used surface water presence/absence data obtained in 2019, and priors for the probability of surface water, based on predictions from an existing regional United States Geological Survey model. Average communication distance posterior distributions revealed locations where effective stream lengths increased dramatically due to flow rarity. We also found strong seasonal (spring, summer, fall) differences in network‐level posterior distributions of both average stream length and average communication distance. Our work demonstrates the unique perspectives concerning network drying provided by communication distance, and demonstrates the general usefulness of Bayesian approaches in the analysis of non‐perennial streams.
Kinesin family member 18B activates mTORC1 signaling via actin gamma 1 to promote the recurrence of human hepatocellular carcinoma
Authors: Li Qian , Sun Mengqing , Meng Yao , Feng Mengqing , Wang Menglan , Chang Cunjie , Dong Heng , Bu Fangtian , Xu Chao , Liu Jing , Ling Qi , Qiao Yiting , Chen Jianxiang
Published: November 2023

The mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway is frequently reported to be hyperactivated in hepatocellular carcinoma (HCC) and contributes to HCC recurrence. However, the underlying regulatory mechanisms of mTORC1 signaling in HCC are not fully understood. In the present study, we found that the expression of kinesin family member 18B (KIF18B) was positively correlated with mTORC1 signaling in HCC, and the upregulation of KIF18B and p-mTOR was associated with a poor prognosis and HCC recurrence. Utilizing in vitro and in vivo assays, we showed that KIF18B promoted HCC cell proliferation and migration through activating mTORC1 signaling. Mechanistically, we identified Actin gamma 1 (γ-Actin) as a binding partner of KIF18B. KIF18B and γ-Actin synergistically modulated lysosome positioning, promoted mTORC1 translocation to lysosome membrane, and prohibited p70 S6K from entering lysosomes for degradation, which finally led to the enhancement of mTORC1 signaling transduction. Moreover, we found that KIF18B was a direct target of Forkhead box M1, which explains the potential mechanism of KIF18B overexpression in HCC. Our study highlights the potential of KIF18B as a therapeutic target for the treatment of HCC.
Evolving treatment patterns and improved outcomes in relapsed/refractory mantle cell lymphoma: a prospective cohort study
Authors: Bock Allison M. , Gile Jennifer J. , Larson Melissa C. , Poonsombudlert Kittika , Tawfiq Reema K. , Maliske Seth , Maurer Matthew J. , Kabat Brian F. , Paludo Jonas , Inwards David J. , Ayyappan Sabarish , Link Brian K. , Ansell Stephen M. , Habermann Thomas M. , Witzig Thomas E. , Nowakowski Grzegorz S. , Cerhan James R. , Farooq Umar , Wang Yucai
Published: November 2023

Over the last two decades, the frontline therapy for mantle cell lymphoma (MCL) has evolved. However, the impact of subsequent lines of therapy on survival outcomes has not been well characterized. In this study, we investigated the treatment patterns and survival outcomes in patients with relapsed/refractory (R/R) MCL treated with second-line (2 L) therapy. Adult patients with newly diagnosed MCL from 2002 to 2015 were enrolled in a prospective cohort study. Clinical characteristics, 2 L treatment details, and outcomes were compared between patients who received 2 L treatment between 2003–2009 (Era 1), 2010–2014 (Era 2), and 2015–2021 (Era 3). 2 L treatment was heterogenous in all eras, and there was a substantial shift in the pattern of 2 L therapy over time. The estimated 2-year EFS rate was 21% (95% CI, 13–35), 40% (95% CI, 30–53), and 51% (95% CI, 37–68) in Era 1–3 respectively, and the 5-year OS rate was 31% (95% CI, 21–45), 37% (95% CI, 27–50), and 67% (95% CI, 54–83) in Era 1–3, respectively. These results provide real-world evidence on evolving treatment patterns of 2 L therapy based on the era of relapse. The changes in 2 L treatment correlated with improved EFS and OS, suggesting that treatment advances are associated with improved outcomes in patients with R/R MCL.
UBE4B interacts with the ITCH E3 ubiquitin ligase to induce Ku70 and c-FLIPL polyubiquitination and enhanced neuroblastoma apoptosis
Authors: Le Clorennec Christophe , Subramonian Divya , Huo Yuchen , Zage Peter E.
Published: November 2023

Expression of the UBE4B ubiquitin ligase is strongly associated with neuroblastoma patient outcomes, but the functional roles of UBE4B in neuroblastoma pathogenesis are not known. We evaluated interactions of UBE4B with the E3 ubiquitin ligase ITCH/AIP4 and the effects of UBE4B expression on Ku70 and c-FLIPL ubiquitination and proteasomal degradation by co-immunoprecipitation and Western blots. We also evaluated the role of UBE4B in apoptosis induced by histone deacetylase (HDAC) inhibition using Western blots. UBE4B binding to ITCH was mediated by WW domains in the ITCH protein. ITCH activation led to ITCH-UBE4B complex formation and recruitment of Ku70 and c-FLIPL via ITCH WW domains, followed by Ku70 and c-FLIPL Lys48/Lys63 branched polyubiquitination and proteasomal degradation. HDAC inhibition induced Ku70 acetylation, leading to release of c-FLIPL and Bax from Ku70, increased Ku70 and c-FLIPL Lys48/Lys63 branched polyubiquitination via the ITCH-UBE4B complex, and induction of apoptosis. UBE4B depletion led to reduced polyubiquitination and increased levels of Ku70 and c-FLIPL and to reduced apoptosis induced by HDAC inhibition via stabilization of c-FLIPL and Ku70 and inhibition of caspase 8 activation. Our results have identified novel interactions and novel targets for UBE4B ubiquitin ligase activity and a direct role for the ITCH-UBE4B complex in responses of neuroblastoma cells to HDAC inhibition, suggesting that the ITCH-UBE4B complex plays a critical role in responses of neuroblastoma to therapy and identifying a potential mechanism underlying the association of UBE4B expression with neuroblastoma patient outcomes.
CLOOME: contrastive learning unlocks bioimaging databases for queries with chemical structures
Authors: Sanchez-Fernandez Ana , Rumetshofer Elisabeth , Hochreiter Sepp , Klambauer Günter
Published: November 2023

The field of bioimage analysis is currently impacted by a profound transformation, driven by the advancements in imaging technologies and artificial intelligence. The emergence of multi-modal AI systems could allow extracting and utilizing knowledge from bioimaging databases based on information from other data modalities. We leverage the multi-modal contrastive learning paradigm, which enables the embedding of both bioimages and chemical structures into a unified space by means of bioimage and molecular structure encoders. This common embedding space unlocks the possibility of querying bioimaging databases with chemical structures that induce different phenotypic effects. Concretely, in this work we show that a retrieval system based on multi-modal contrastive learning is capable of identifying the correct bioimage corresponding to a given chemical structure from a database of ~2000 candidate images with a top-1 accuracy >70 times higher than a random baseline. Additionally, the bioimage encoder demonstrates remarkable transferability to various further prediction tasks within the domain of drug discovery, such as activity prediction, molecule classification, and mechanism of action identification. Thus, our approach not only addresses the current limitations of bioimaging databases but also paves the way towards foundation models for microscopy images.
Mitigating stimulated Brillouin scattering in multimode fibers with focused output via wavefront shaping
Authors: Chen Chun-Wei , Nguyen Linh V. , Wisal Kabish , Wei Shuen , Warren-Smith Stephen C. , Henderson-Sapir Ori , Schartner Erik P. , Ahmadi Peyman , Ebendorff-Heidepriem Heike , Stone A. Douglas , Ottaway David J. , Cao Hui
Published: November 2023

The key challenge for high-power delivery through optical fibers is overcoming nonlinear optical effects. To keep a smooth output beam, most techniques for mitigating optical nonlinearities are restricted to single-mode fibers. Moving out of the single-mode paradigm, we show experimentally that wavefront-shaping of coherent input light to a highly multimode fiber can increase the power threshold for stimulated Brillouin scattering (SBS) by an order of magnitude, whilst simultaneously controlling the output beam profile. The SBS suppression results from an effective broadening of the Brillouin spectrum under multimode excitation, without broadening of transmitted light. Strongest suppression is achieved with selective mode excitation that gives the broadest Brillouin spectrum. Our method is efficient, robust, and applicable to continuous waves and pulses. This work points toward a promising route for mitigating detrimental nonlinear effects in optical fibers, enabling further power scaling of high-power fiber systems for applications to directed energy, remote sensing, and gravitational-wave detection.
Local membrane source gathering by p62 body drives autophagosome formation
Authors: Feng Xuezhao , Sun Daxiao , Li Yanchang , Zhang Jinpei , Liu Shiyu , Zhang Dachuan , Zheng Jingxiang , Xi Qing , Liang Haisha , Zhao Wenkang , Li Ying , Xu Mengbo , He Jiayu , Liu Tong , Hasim Ayshamgul , Ma Meisheng , Xu Ping , Mi Na
Published: November 2023

Autophagosomes are double-membrane vesicles generated intracellularly to encapsulate substrates for lysosomal degradation during autophagy. Phase separated p62 body plays pivotal roles during autophagosome formation, however, the underlying mechanisms are still not fully understood. Here we describe a spatial membrane gathering mode by which p62 body functions in autophagosome formation. Mass spectrometry-based proteomics reveals significant enrichment of vesicle trafficking components within p62 body. Combining cellular experiments and biochemical reconstitution assays, we confirm the gathering of ATG9 and ATG16L1-positive vesicles around p62 body, especially in Atg2ab DKO cells with blocked lipid transfer and vesicle fusion. Interestingly, p62 body also regulates ATG9 and ATG16L vesicle trafficking flux intracellularly. We further determine the lipid contents associated with p62 body via lipidomic profiling. Moreover, with in vitro kinase assay, we uncover the functions of p62 body as a platform to assemble ULK1 complex and invigorate PI3KC3-C1 kinase cascade for PI3P generation. Collectively, our study raises a membrane-based working model for multifaceted p62 body in controlling autophagosome biogenesis, and highlights the interplay between membraneless condensates and membrane vesicles in regulating cellular functions.