bluuebunny/crossref_metadata_2025 huggingface dataset:
Anti-LAMP-2 Antibody Seropositivity in Children with Primary Systemic Vasculitis Affecting Medium- and Large-Sized Vessels
Authors: Akbaba Tayfun Hilmi , Toor Kirandeep K. , Mann Simranpreet K. , Gibson Kristen M. , Alfaro Gabriel Alejandro , Balci-Peynircioglu Banu , Cabral David A. , Morishita Kimberly A. , Brown Kelly L.
Published: March 2024

Chronic primary systemic vasculitis (PSV) comprises a group of heterogeneous diseases that are broadly classified by affected blood vessel size, clinical traits and the presence (or absence) of anti-neutrophil cytoplasmic antibodies (ANCA) against proteinase 3 (PR3) and myeloperoxidase (MPO). In small vessel vasculitis (SVV), ANCA are not present in all patients, and they are rarely detected in patients with vasculitis involving medium (MVV) and large (LVV) blood vessels. Some studies have demonstrated that lysosome-associated membrane protein-2 (LAMP-2/CD107b) is a target of ANCA in SVV, but its presence and prognostic value in childhood MVV and LVV is not known. This study utilized retrospective sera and clinical data obtained from 90 children and adolescents with chronic PSV affecting small (SVV, n = 53), medium (MVV, n = 16), and large (LVV, n = 21) blood vessels. LAMP-2-ANCA were measured in time-of-diagnosis sera using a custom electrochemiluminescence assay. The threshold for seropositivity was established in a comparator cohort of patients with systemic autoinflammatory disease. The proportion of LAMP-2-ANCA-seropositive individuals and sera concentrations of LAMP-2-ANCA were assessed for associations with overall and organ-specific disease activity at diagnosis and one-year follow up. This study demonstrated a greater time-of-diagnosis prevalence and sera concentration of LAMP-2-ANCA in MVV (52.9% seropositive) and LVV (76.2%) compared to SVV (45.3%). Further, LAMP-2-ANCA-seropositive individuals had significantly lower overall, but not organ-specific, disease activity at diagnosis. This did not, however, result in a greater reduction in disease activity or the likelihood of achieving inactive disease one-year after diagnosis. The results of this study demonstrate particularly high prevalence and concentration of LAMP-2-ANCA in chronic PSV that affects large blood vessels and is seronegative for traditional ANCA. Our findings invite reconsideration of roles for autoantigens other than MPO and PR3 in pediatric vasculitis, particularly in medium- and large-sized blood vessels.
Attenuation of Polycyclic Aromatic Hydrocarbon (PAH)-Induced Carcinogenesis and Tumorigenesis by Omega-3 Fatty Acids in Mice In Vivo
Authors: Xia Guobin , Zhou Guodong , Jiang Weiwu , Chu Chun , Wang Lihua , Moorthy Bhagavatula
Published: March 2024

Lung cancer is the leading cause of cancer death worldwide. Polycyclic aromatic hydrocarbons (PAHs) are metabolized by the cytochrome P450 (CYP)1A and 1B1 to DNA-reactive metabolites, which could lead to mutations in critical genes, eventually resulting in cancer. Omega-3 fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are beneficial against cancers. In this investigation, we elucidated the mechanisms by which omega-3 fatty acids EPA and DHA will attenuate PAH-DNA adducts and lung carcinogenesis and tumorigenesis mediated by the PAHs BP and MC. Adult wild-type (WT) (A/J) mice, Cyp1a1-null, Cyp1a2-null, or Cyp1b1-null mice were exposed to PAHs benzo[a]pyrene (BP) or 3-methylcholanthrene (MC), and the effects of omega-3 fatty acid on PAH-mediated lung carcinogenesis and tumorigenesis were studied. The major findings were as follows: (i) omega-3 fatty acids significantly decreased PAH-DNA adducts in the lungs of each of the genotypes studied; (ii) decreases in PAH-DNA adduct levels by EPA/DHA was in part due to inhibition of CYP1B1; (iii) inhibition of soluble epoxide hydrolase (sEH) enhanced the EPA/DHA-mediated prevention of pulmonary carcinogenesis; and (iv) EPA/DHA attenuated PAH-mediated carcinogenesis in part by epigenetic mechanisms. Taken together, our results suggest that omega-3 fatty acids have the potential to be developed as cancer chemo-preventive agents in people.
Convergent Mutations and Single Nucleotide Variants in Mitochondrial Genomes of Modern Humans and Neanderthals
Authors: Ferreira Renata C. , Rodrigues Camila R. , Broach James R. , Briones Marcelo R. S.
Published: March 2024

The genetic contributions of Neanderthals to the modern human genome have been evidenced by the comparison of present-day human genomes with paleogenomes. Neanderthal signatures in extant human genomes are attributed to intercrosses between Neanderthals and archaic anatomically modern humans (AMHs). Although Neanderthal signatures are well documented in the nuclear genome, it has been proposed that there is no contribution of Neanderthal mitochondrial DNA to contemporary human genomes. Here we show that modern human mitochondrial genomes contain 66 potential Neanderthal signatures, or Neanderthal single nucleotide variants (N-SNVs), of which 36 lie in coding regions and 7 result in nonsynonymous changes. Seven N-SNVs are associated with traits such as cycling vomiting syndrome, Alzheimer’s disease and Parkinson’s disease, and two N-SNVs are associated with intelligence quotient. Based on recombination tests, principal component analysis (PCA) and the complete absence of these N-SNVs in 41 archaic AMH mitogenomes, we conclude that convergent evolution, and not recombination, explains the presence of N-SNVs in present-day human mitogenomes.
Phytotherapy of Vulvovaginal Candidiasis: A Narrative Review
Authors: Picheta Natalia , Piekarz Julia , Burdan Oliwia , Satora Małgorzata , Tarkowski Rafał , Kułak Krzysztof
Published: March 2024

Vulvovaginal candidiasis (VVC) is a real gynecological problem among women of reproductive age from 15 to 49. A recent analysis showed that 75% of women will have an occurrence at least once per year, while 5% are observed to have recurrent vaginal mycosis—these patients may become unwell four or more times a year. This pathology is caused in 85–90% of cases by fungi of the Candida albicans species. It represents an intractable medical problem for female patients due to pain and pruritus. Due to the observation of an increasing number of strains resistant to standard preparations and an increase in the recurrence of this pathology when using local or oral preferential therapy, such as fluconazole, an analysis was launched to develop alternative methods of treating VVC using herbs such as dill, turmeric, and berberine. An in-depth analysis of databases that include scientific articles from recent years made it possible to draw satisfactory conclusions supporting the validity of herbal therapy for the pathology in question. Although phytotherapy has not yet been approved by the Food and Drug Administration, it appears to be a promising therapeutic solution for strains that are resistant to existing treatments. There is research currently undergoing aimed at comparing classical pharmacotherapy and herbal therapy in the treatment of vaginal candidiasis for the purpose of increasing medical competence and knowledge for the care of the health and long-term comfort of gynecological patients.
Control Potential of Multiple Nucleopolyhedrovirus (SfMNPV) Isolated from Fall Armyworm in Nigeria (West Africa)
Authors: Tepa-Yotto Ghislain T. , Douro-Kpindou Ouorou Kobi , Koussihouédé Précieux Sèna Bonaventure , Adjaoké Abissi Marc , Winsou Jeannette K. , Tognigban Ghislain , Tamò Manuele
Published: March 2024

The fall armyworm (FAW) Spodoptera frugiperda (Lepidoptera, Noctuidae) has now become an invasive pest of global concern. The pest was first detected in Central and Western Africa in early 2016. Sustainable management options explored by stakeholders during early FAW invasion in Africa included the use of biopesticides and biological control. The current study aimed to compare the susceptibility of FAW larvae to SfMNPV with the assumption that the virus isolated from FAW populations in Africa has higher virulence compared with an isolate from Argentina (SfMNPV-ARG). We also hypothesized that host plant plays a role in SfMNPV efficacy and that cannibalism mediates horizontal and vertical transmission of the virus. This work provides pioneering data on the virulence of the new SfMNPV isolate from Nigeria (SfMNPV-KA1), which proved more effective than its exotic counterpart from Argentina (SfMNPV-ARG). The host plant effect made a significant difference between maize and onion with more FAW death in the larvae fed with contaminated onion 5 days post treatment. The study demonstrates and discusses the effect of cannibalism on virus transmission.
The Emergence of the Family Scirtidae (Insecta: Coleoptera) in Lotic Karst Habitats: A Case Study over 15 Years
Authors: Klarin Ana , Ivković Marija , Mičetić Stanković Vlatka
Published: March 2024

Due to ongoing changes and a decline in biodiversity, science today should rely on long-term species-based ecological studies. We have conducted a long-term ecological dynamics study on the water beetle family Scirtidae, which, although it is very abundant in benthic communities, is still poorly studied. The main objective of this study was to investigate the population aspects (composition, diversity, sex ratio) and ecological aspects (emergence patterns, seasonal dynamics and preferences for environmental factors) of the family Scirtidae over 15 years in Plitvice Lakes NP, Croatia. The study was conducted at three sites and in five different substrate types. A total of three taxa with different distributions were recorded in the study area: Hydrocyphon novaki and H. deflexicollis on the tufa barriers and the Elodes sp. in the spring area. The sex ratio was in favour of males in spring and early summer, while it shifted in favour of females towards the end of autumn. The abundance and emergence of the family were primarily determined by the environmental parameters which showed the greatest fluctuations over a period of 15 years: water temperature, water discharge and oxygen saturation. Our results clearly show that Scirtidae can be used as indicators of stream zonation and habitat quality. Based on the methodology and the results of this study, we conclude that Scirtidae should be used in future monitoring and protection measures in karst freshwater habitats in southeastern Europe.
Inhibition Effect of Non-Host Plant Volatile Extracts on Reproductive Behaviors in the Diamondback Moth Plutella xylostella (Linnaeus)
Authors: Zhou Junxiang , Zhang Zhen , Liu Haotian , Guo Mengbo , Deng Jianyu
Published: March 2024

The pest management of Plutella xylostella, the global pest of cruciferous plants, is primarily dependent upon continued applications of insecticides, which has led to severe insecticide resistance and a series of ecological concerns. The essential oils (EOs) of non-host plants are considered to have a high application potential in pest behavioral control. In P. xylostella, the insecticidal properties, antifeeding activities, and oviposition inhibition effects of many EOs have been studied in larvae and female moths. However, less focus has been placed on the inhibitory effect on sex pheromone communication during courtship, which is vital for the reproduction of the offspring. In this study, by combining electrophysiological studies, laboratory behavioral assays, and field traps, we demonstrated that non-host plant EOs significantly inhibited the reproductive behaviors of both sexes. Notably, the calamus (Acorus gramineus) EO inhibited the preference of male moths for synthetic sex pheromone blends and reduced the egg-laying number of female moths on host plants, with the highest inhibition rates of 72% and 100%, respectively, suggesting a great application prospect of calamus and its EO on the behavioral control strategies of P. xylostella.
Diversity of Parasitoid Wasps and Comparison of Sampling Strategies in Rice Fields Using Metabarcoding
Authors: Wang Liyang , Wu Hongxuan , He Wei , Lai Guihong , Li Junxi , Liu Siling , Zhou Qiang
Published: March 2024

A comprehensive and precise evaluation of Arthropoda diversity in agricultural landscapes can enhance biological pest control strategies. We used Malaise traps and sweep nets to collect insects from three double-cropping paddy fields. DNA was extracted from the ethanol preservative of the Malaise traps and from tissue samples of selected parasitoid wasps. This was followed by amplification using DNA barcoding primers to prepare high-throughput sequencing libraries. We annotated a total of 4956 operational taxonomic units (OTUs), encompassing 174 genera and 32 families of parasitoid wasps. The ethanol filter method efficiently captured a wide range of information. However, the method has low resolution and may result in a reduced estimate of species abundance. Additional insect species were also identified in the parasitoid samples. This suggests that high throughput sequencing from adult parasitoid wasps can also detect host species, enabling a better understanding of host species and providing insights into food webs.
Six Steps towards a Spatial Design for Large-Scale Pollinator Surveillance Monitoring
Authors: Hellwig Niels , Sommerlandt Frank M. J. , Grabener Swantje , Lindermann Lara , Sickel Wiebke , Krüger Lasse , Dieker Petra
Published: March 2024

Despite the importance of pollinators to ecosystem functioning and human food production, comprehensive pollinator monitoring data are still lacking across most regions of the world. Policy-makers have recently prioritised the development of large-scale monitoring programmes for pollinators to better understand how populations respond to land use, environmental change and restoration measures in the long term. Designing such a monitoring programme is challenging, partly because it requires both ecological knowledge and advanced knowledge in sampling design. This study aims to develop a conceptual framework to facilitate the spatial sampling design of large-scale surveillance monitoring. The system is designed to detect changes in pollinator species abundances and richness, focusing on temperate agroecosystems. The sampling design needs to be scientifically robust to address questions of agri-environmental policy at the scales of interest. To this end, we followed a six-step procedure as follows: (1) defining the spatial sampling units, (2) defining and delimiting the monitoring area, (3) deciding on the general sampling strategy, (4) determining the sample size, (5) specifying the sampling units per sampling interval, and (6) specifying the pollinator survey plots within each sampling unit. As a case study, we apply this framework to the “Wild bee monitoring in agricultural landscapes of Germany” programme. We suggest this six-step procedure as a conceptual guideline for the spatial sampling design of future large-scale pollinator monitoring initiatives.
Dual Effects of Cold Storage and Stored Host Eggs of Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) on the Reproductive Capacity of Telenomus remus Nixon (Hymenoptera: Scelionidae)
Authors: Qiu Ranran , Li Jun , Desneux Nicolas , Zang Liansheng , He Xiaofang , Lü Xin
Published: March 2024

Spodoptera frugiperda is the preferred host of the parasitoid Telenomus remus. Cold storage can preserve a sufficient quantity of parasitoids and their hosts in a laboratory colony for mass release. First, the effects of cold storage on the reproductive capacity of T. remus reared on non-stored S. frugiperda eggs and the hatching rate of unparasitized S. frugiperda eggs were investigated. Further, the dual effects of cold storage and stored S. frugiperda eggs on the reproductive capacity of T. remus were studied to determine the optimal storage conditions and the maximal shelf life for both the host and the parasitoid. The emergence rate, the number of adults produced, and the female proportion of T. remus were affected by cold storage factors. Pupae stored at 13 °C for 15 days is optimum for T. remus reared on non-stored S. frugiperda eggs. Spodoptera frugiperda eggs could only be stored at 10 °C for five days to be suitable for rearing T. remus. The optimum cold storage conditions for T. remus parasitizing stored eggs were 7 °C for 5 days in the larval stage. The maximal shelf lives of T. remus parasitizing cold-stored S. frugiperda eggs were 10 days. Cold storage affected the hatching rate of S. frugiperda eggs, thereby further affecting the reproductive capacity of T. remus. The findings suggest that different storage conditions should be used when mass-rearing T. remus on stored and non-stored eggs. Telenomus remus should be reproduced using fresh laid S. frugiperda eggs for maximum shelf life.
Repurposing the Fibrosis-4 Score in Rheumatoid Arthritis: Data from the ESPOIR Cohort
Authors: Felten Renaud , Fabacher Thibaut , Sedmak Nathanaël , Sibilia Jean , Sordet Christelle , Chatelus Emmanuel , Berenbaum Francis , Combe Bernard , Ruyssen-Witrand Adeline , Vittecoq Olivier , Meyer Nicolas , Gottenberg Jacques-Eric
Published: March 2024

Background: We aimed to evaluate the value of the Fibrosis-4 (FIB-4) score as a prognostic factor in RA in the prospective ESPOIR cohort. Methods: We included patients from the ESPOIR cohort with a diagnosis of RA according to ACR/EULAR criteria. The formula for the FIB-4 score is as follows: [age (years) × aspartate transaminase level (U/L)]/[platelet count (109/L) × alanine aminotransferase level (U/L)1/2]. We used a linear mixed-effects model with a random effect of patient to account for repeated measures over time. Results: Overall, 647 of the 813 patients included met the ACR/EULAR criteria for RA, with no differential diagnosis during the first 10 years of follow-up. Of these patients, at baseline, 633 had a calculable FIB-4 score. Median FIB-4 score was 0.75 (interquartile range 0.53–0.99). On multivariate analysis, FIB-4 score was not independently associated with progression of Disease Activity Score in 28 joints over 10 years of follow-up, unlike baseline C-reactive protein level and SJC. Baseline FIB-4 score was not associated with the modified Sharp score at 5-year follow-up, unlike age and ACPAs. FIB-4 score was not associated with mortality (hazard ratio 1.1 [95% CI 0.46; 2.8], p = 0.77) or major adverse cardiovascular events (0.46 [0.13; 1.6], p = 0.22) over the 10-year follow-up. No significant change in FIB-4 score over time was related to treatments. Conclusions: The present prospective cohort study did not find a prognostic role of FIB-4 score in RA. Reassuringly, FIB-4 score was not increased with DMARD treatment after 10 years of follow-up.
Long COVID Clinical Severity Types Based on Symptoms and Functional Disability: A Longitudinal Evaluation
Authors: Sivan Manoj , Smith Adam B. , Osborne Thomas , Goodwin Madeline , Lawrence Román Rocha , Baley Sareeta , Williams Paul , Lee Cassie , Davies Helen , Balasundaram Kumaran , Greenwood Darren C.
Published: March 2024

Background: Long COVID (LC) is a multisystem clinical syndrome with functional disability and compromised overall health. Information on LC clinical severity types is emerging in cross-sectional studies. This study explored the pattern and consistency of long COVID (LC) clinical severity types over time in a prospective sample. Methods: Participants with LC completed the condition-specific outcome measure C19-YRSm (Yorkshire Rehabilitation Scale modified version) at two assessment time points. A cluster analysis for clinical severity types was undertaken at both time points using the k-means partition method. Results: The study included cross-sectional data for 759 patients with a mean age of 46.8 years (SD = 12.7), 69.4% females, and a duration of symptoms of 360 days (IQR 217 to 703 days). The cluster analysis at first assessment revealed three distinct clinical severity type clusters: mild (n = 96), moderate (n = 422), and severe (n = 241). Longitudinal data on 356 patients revealed that the pattern of three clinical severity types remained consistent over time between the two assessments, with 51% of patients switching clinical severity types between the assessments. Conclusions: This study is the first of its kind to demonstrate that the pattern of three clinical severity types is consistent over time, with patients also switching between severity types, indicating the fluctuating nature of LC.
Hypernatremia in Hyperglycemia: Clinical Features and Relationship to Fractional Changes in Body Water and Monovalent Cations during Its Development
Authors: Wagner Brent , Ing Todd S. , Roumelioti Maria-Eleni , Sam Ramin , Argyropoulos Christos P. , Lew Susie Q. , Unruh Mark L. , Dorin Richard I. , Degnan James H. , Tzamaloukas Antonios H.
Published: March 2024

In hyperglycemia, the serum sodium concentration ([Na]S) receives influences from (a) the fluid exit from the intracellular compartment and thirst, which cause [Na]S decreases; (b) osmotic diuresis with sums of the urinary sodium plus potassium concentration lower than the baseline euglycemic [Na]S, which results in a [Na]S increase; and (c), in some cases, gains or losses of fluid, sodium, and potassium through the gastrointestinal tract, the respiratory tract, and the skin. Hyperglycemic patients with hypernatremia have large deficits of body water and usually hypovolemia and develop severe clinical manifestations and significant mortality. To assist with the correction of both the severe dehydration and the hypovolemia, we developed formulas computing the fractional losses of the body water and monovalent cations in hyperglycemia. The formulas estimate varying losses between patients with the same serum glucose concentration ([Glu]S) and [Na]S but with different sums of monovalent cation concentrations in the lost fluids. Among subjects with the same [Glu]S and [Na]S, those with higher monovalent cation concentrations in the fluids lost have higher fractional losses of body water. The sum of the monovalent cation concentrations in the lost fluids should be considered when computing the volume and composition of the fluid replacement for hyperglycemic syndromes.
Single Nucleotide Polymorphisms of CYP3A4 and CYP3A5 in Romanian Kidney Transplant Recipients: Effect on Tacrolimus Pharmacokinetics in a Single-Center Experience
Authors: Rotarescu Corina Andreea , Maruntelu Ion , Rotarescu Ion , Constantinescu Alexandra-Elena , Constantinescu Ileana
Published: March 2024

Background: This study examines the impact of CYP3A4 and CYP 3A5 genotypes on tacrolimus (Tac) pharmacokinetics in Romanian kidney transplanted patients. Methods: We included 112 kidney recipients genotyped for CYP3A5*3, CYP3A4*1.001, and CYP3A4*22. Patients were categorized into poor, intermediate, rapid, and ultra-rapid metabolizers based on the functional defects linked to CYP3A variants. Results: Predominantly male (63.4%) with an average age of 40.58 years, the cohort exhibited a high prevalence of the CYP3A4*1/*1 (86.6%) and CYP3A5*3/*3 (77.7%) genotypes. CYP3A4*1.001 and CYP3A5*1 alleles significantly influenced the Tac concentration-to-dose (C0/D) ratio in various post-transplant periods, while the CYP3A4*22 allele showed no such effect (p = 0.016, p < 0.001). Stepwise regression highlighted the CYP3A4*1.001’s impact in early post-transplant phases, with hematocrit and age also influencing Tac variability. Conclusions: The study indicates a complex interaction of CYP3A4 and CYP3A5 genotypes on Tac metabolism, suggesting the necessity for personalized medication approaches based on genetic profiling in kidney transplant recipients.
Beyond the Cold: Activating Brown Adipose Tissue as an Approach to Combat Obesity
Authors: Negroiu Cristina Elena , Tudorașcu Iulia , Bezna Cristina Maria , Godeanu Sanziana , Diaconu Marina , Danoiu Raluca , Danoiu Suzana
Published: March 2024

With a dramatic increase in the number of obese and overweight people, there is a great need for new anti-obesity therapies. With the discovery of the functionality of brown adipose tissue in adults and the observation of beige fat cells among white fat cells, scientists are looking for substances and methods to increase the activity of these cells. We aimed to describe how scientists have concluded that brown adipose tissue is also present and active in adults, to describe where in the human body these deposits of brown adipose tissue are, to summarize the origin of both brown fat cells and beige fat cells, and, last but not least, to list some of the substances and methods classified as BAT promotion agents with their benefits and side effects. We summarized these findings based on the original literature and reviews in the field, emphasizing the discovery, function, and origins of brown adipose tissue, BAT promotion agents, and batokines. Only studies written in English and with a satisfying rating were identified from electronic searches of PubMed.
A Recent Development of a Network Approach to Assessment Data: Latent Space Item Response Modeling for Intelligence Studies
Authors: Kang Inhan , Jeon Minjeong
Published: March 2024

This article aims to provide an overview of the potential advantages and utilities of the recently proposed Latent Space Item Response Model (LSIRM) in the context of intelligence studies. The LSIRM integrates the traditional Rasch IRT model for psychometric data with the latent space model for network data. The model has person-wise latent abilities and item difficulty parameters, capturing the main person and item effects, akin to the Rasch model. However, it additionally assumes that persons and items can be mapped onto the same metric space called a latent space and distances between persons and items represent further decreases in response accuracy uncaptured by the main model parameters. In this way, the model can account for conditional dependence or interactions between persons and items unexplained by the Rasch model. With two empirical datasets, we illustrate that (1) the latent space can provide information on respondents and items that cannot be captured by the Rasch model, (2) the LSIRM can quantify and visualize potential between-person variations in item difficulty, (3) latent dimensions/clusters of persons and items can be detected or extracted based on their latent positions on the map, and (4) personalized feedback can be generated from person-item distances. We conclude with discussions related to the latent space modeling integrated with other psychometric models and potential future directions.
A Digital Twin for Assessing the Remaining Useful Life of Offshore Wind Turbine Structures
Authors: Pacheco-Blazquez Rafael , Garcia-Espinosa Julio , Di Capua Daniel , Pastor Sanchez Andres
Published: March 2024

This paper delves into the application of digital twin monitoring techniques for enhancing offshore floating wind turbine performance, with a detailed case study that uses open-source digital twin software. We explore the practical implementation of digital twins and their efficacy in optimizing operations and predictive maintenance, focusing on controlling the real-time structural state of composite wind turbine structures and forecasting the remaining useful life by tracking the fatigue state in the structure. Our findings emphasize digital twins’ potential as a valuable tool for renewable energy, driving efficiency and sustainability in offshore floating wind installations. These aspects, along with the aforementioned simulations, whether in real-time or forecasted, reduce costly and unnecessary inspections and scheduled maintenance.
Transcriptome-Wide Identification and Expression Analysis of Genes Encoding Defense-Related Peptides of Filipendula ulmaria in Response to Bipolaris sorokiniana Infection
Authors: Istomina Ekaterina A. , Korostyleva Tatyana V. , Kovtun Alexey S. , Slezina Marina P. , Odintsova Tatyana I.
Published: March 2024

Peptides play an essential role in plant development and immunity. Filipendula ulmaria, belonging to the Rosaceae family, is a medicinal plant which exhibits valuable pharmacological properties. F. ulmaria extracts in vitro inhibit the growth of a variety of plant and human pathogens. The role of peptides in defense against pathogens in F. ulmaria remains unknown. The objective of this study was to explore the repertoire of antimicrobial (AMPs) and defense-related signaling peptide genes expressed by F. ulmaria in response to infection with Bipolaris sorokiniana using RNA-seq. Transcriptomes of healthy and infected plants at two time points were sequenced on the Illumina HiSeq500 platform and de novo assembled. A total of 84 peptide genes encoding novel putative AMPs and signaling peptides were predicted in F. ulmaria transcriptomes. They belong to known, as well as new, peptide families. Transcriptional profiling in response to infection disclosed complex expression patterns of peptide genes and identified both up- and down-regulated genes in each family. Among the differentially expressed genes, the vast majority were down-regulated, suggesting suppression of the immune response by the fungus. The expression of 13 peptide genes was up-regulated, indicating their possible involvement in triggering defense response. After functional studies, the encoded peptides can be used in the development of novel biofungicides and resistance inducers.
Personalized Research on the Aging Face—A Narrative History
Authors: Hînganu Marius Valeriu , Cucu Ramona Paula , Hînganu Delia
Published: March 2024

Throughout history, people have struggled to find out the secret of youth. The aim of the manuscript is to review the main achievements regarding the exploration of the aging face phenomenon. It should be very important to know the evolution in this field due to the increase in life expectancy among the population. Our purpose is for the current study to serve as a starting point towards exploring novel research avenues in molecular biology and the confocal immunofluorescence of cervicofacial soft tissues, employing cutting-edge techniques. All changes in the shape of the facial skeleton, soft tissue, retaining ligaments, fat compartments, and the skin envelope contribute to facial aging to varying degrees.
The Impact of Periodontal Disease on Preterm Birth and Preeclampsia
Authors: Tsikouras Panagiotis , Oikonomou Efthymios , Nikolettos Konstantinos , Andreou Sotiris , Kyriakou Dimitrios , Damaskos Christos , Garmpis Nikolaos , Monastiridou Vassiliki , Nalmpanti Theopi , Bothou Anastasia , Iatrakis George , Nikolettos Nikolaos
Published: March 2024

This review delves into the possible connection between periodontitis and negative pregnancy outcomes, such as preeclampsia and preterm birth. It highlights the potential influence of an unidentified microbial factor on preeclampsia and the effects of inflammatory responses on the rate of preterm births. Furthermore, it underscores the prevalent occurrence of oral ailments within the populace and their significant repercussions on quality of life. Hormonal fluctuations during pregnancy may exacerbate oral conditions such as pregnancy gingivitis and periodontitis, necessitating bespoke therapeutic approaches that take into account potential fetal ramifications. Periodontal disease, characterized by microbial attack and inflammatory response, results in tissue destruction and tooth loss. The oral cavity’s susceptibility to bacterial colonization, which is primarily due to its role as a site for food intake, is highlighted. Furthermore, research indicates a correlation between inflammatory responses and factors such as prostaglandin E2 and IL-1β, and preterm birth. Therapeutic interventions are a focus of international research, with efforts being aimed at optimizing outcomes through larger studies involving pregnant women.
Effects of Hyperbaric Oxygen Therapy on Long COVID: A Systematic Review
Authors: Wu Bing-Qi , Liu De-Yi , Shen Te-Chun , Lai Yu-Ru , Yu Tsai-Ling , Hsu Hsiang-Li , Lee Hsiu-Ming , Liao Wei-Chih , Hsia Te-Chun
Published: March 2024

The coronavirus disease (COVID-19) pandemic has resulted in an increasing population that is experiencing a wide range of long-lasting symptoms after recovery from the acute infection. Long COVID refers to this specific condition and is associated with diverse symptoms, such as fatigue, myalgias, dyspnea, headache, cognitive impairment, neurodegenerative symptoms, anxiety, depression, and a sense of despair. The potential of hyperbaric oxygen therapy (HBOT) to improve chronic fatigue, cognitive impairments, and neurological disorders has been established; therefore, the use of HBOT to treat long COVID has also been studied. We conducted a literature search between 1 January 2019 and 30 October 2023, focusing on the clinical efficacy and utility of HBOT for treating long COVID and found ten clinical studies that fit the review topic, including one case report, five one-group pretest-posttest design studies, one safety report from a randomized controlled trial (RCT), and three complete reports of RCTs. Most studies found that HBOT can improve quality of life, fatigue, cognition, neuropsychiatric symptoms, and cardiopulmonary function. Although HBOT has shown some benefits for long COVID symptoms, further rigorous large-scale RCTs are required to establish precise indications, protocols, and post-treatment evaluations.
Changes in EEG Activity and Cognition Related to Physical Activity in Older Adults: A Systematic Review
Authors: Rodríguez-Serrano Luis Miguel , Wöbbeking-Sánchez Marina , De La Torre Lizbeth , Pérez-Elvira Ruben , Chávez-Hernández María Elena
Published: March 2024

Aging is generally associated with a decline in important cognitive functions that can be observed in EEG. Physical activity in older adults should be considered one of the main strategies to promote health and prevent disease in the elderly. The present study aimed to systematically review studies of EEG activity and cognitive function changes associated with physical activity in older adults. Records from PubMed, Scopus, and EBSCO databases were searched and, following the PRISMA guidelines, nine studies were included in the present systematic review. A risk of bias assessment was performed using the National Institute of Health Quality Assessment Tool for Case-control Studies instrument. The studies analyzed used two main strategies to determine the effects of physical activity on cognition and EEG: (1) multiscale entropy and power frequencies; and (2) event-related potentials. In terms of EEG activity, it can be concluded that exercise-induced neuroplasticity underlies improvements in cognitive function in healthy older adults.
Aza-Diphosphido-Bridged Di-Iron Complexes Related to the [FeFe]-Hydrogenases
Authors: Das Pankaj , Elleouet Catherine , Pétillon François Y. , Schollhammer Philippe
Published: March 2024

The reaction of the dianionic species [Fe2(CO)6(μ-PPh)2]2− with tBuN(CH2Cl)2 gives the di-iron carbonyl aza-diphosphido-bridged complex [Fe2(CO)6(µ-{P(Ph)CH2}2NtBu)] (1). Attempts to prepare 1 by click-chemistry by reacting [Fe2(CO)6(μ-PHPh)2] with CH2O and tBuNH2 afforded a bis-phosphido compound [Fe2(CO)6(µ-P(Ph)CH2NHtBu)2] (2) which exists as two, syn and anti, isolable isomers depending on the relative orientation of the groups carried by the phosphorus atoms. In the presence of HBF4.Et2O, in dichloromethane, 1 leads to the stabilized ammonium species [Fe2(CO)6(µ-{P(Ph)CH2}2NHtBu)](BF4) (3). The derivatives 1–3 were characterized by IR and 1H, 31P-{1H} NMR spectroscopies. Their structures in a solid state were determined by X-ray diffraction analyses, which accord with their spectroscopic characteristics.
Abrasive Wear Properties of Wear-Resistant Coating on Bucket Teeth Assessed Using a Dry Sand Rubber Wheel Tester
Authors: Wang Zhongxin , Sun Long , Wang Dong , Song Bo , Liu Chang , Su Zhenning , Ma Chaobin , Ren Xiaoyong
Published: March 2024

Ni60-WC coatings with different WC contents on the bucket tooth substrates were pre- pared using laser cladding technology. Their abrasive wear properties were assessed using the dry sand rubber wheel test system. The substrate and the hard-facing layer were tested for comparison. The results showed that the hardness of the Ni60-WC coatings increased with the increase in WC content. The wear resistance of the bucket tooth substrate was greatly improved by hard-facing and laser cladding Ni60-WC coatings. The wear rate of the hard-facing layer was reduced to 1/6 of that of the tooth substrate. The wear rate of the laser cladding coatings with 20–40 wt.% WC was similar to that of the hard-facing layer. It is worth mentioning that the wear rate of the coatings with 60–80 wt.% WC was only 1/4 of that of the hard-facing layer. Micro-cutting with surface plastic deformation was the main wear mechanism of the substrate to form narrow and deep furrows. The wear mechanism of the hard-facing layer was mainly plastic deformation with a wide groove, and the surface cracks promoted the removal of the material. The removal of the binder phase caused by micro-cutting was the main wear mechanism of the laser cladding Ni60-WC coatings. However, the hard phase of WC hinders micro-cutting and plastic deformation, which improves the wear resistance of the coating.
Characterization of Microstructure, Optical Properties, and Mechanical Behavior of a Temporary 3D Printing Resin: Impact of Post-Curing Time
Authors: Siqueira Joyce Roma Correia dos Santos , Rodriguez Rita Maria Morejon , Campos Tiago Moreira Bastos , Ramos Nathalia de Carvalho , Bottino Marco Antonio , Tribst João Paulo Mendes
Published: March 2024

The present study aimed to characterize the microstructure of a temporary 3D printing polymer-based composite material (Resilab Temp), evaluating its optical properties and mechanical behavior according to different post-curing times. For the analysis of the surface microstructure and establishment of the best printing pattern, samples in bar format following ISO 4049 (25 × 10 × 3 mm) were designed in CAD software (Rhinoceros 6.0), printed on a W3D printer (Wilcos), and light-cured in Anycubic Photon for different lengths of time (no post-curing, 16 min, 32 min, and 60 min). For the structural characterization, analyses were carried out using Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The mechanical behavior of this polymer-based composite material was determined based on flexural strength tests and Knoop microhardness. Color and translucency analysis were performed using a spectrophotometer (VITA Easy Shade Advanced 4.0), which was then evaluated in CIELab, using gray, black, and white backgrounds. All analyses were performed immediately after making the samples and repeated after thermal aging over two thousand cycles (5–55 °C). The results obtained were statistically analyzed with a significance level of 5%. FT-IR analysis showed about a 46% degree of conversion on the surface and 37% in the center of the resin sample. The flexural strength was higher for the groups polymerized for 32 min and 1 h, while the Knoop microhardness did not show a statistical difference between the groups. Color and translucency analysis also did not show statistical differences between groups. According to all of the analyses carried out in this study, for the evaluated material, a post-polymerization time of 1 h should be suggested to improve the mechanical performance of 3D-printed devices.
Computational Evaluation of the Fracture Behavior of Porous Asphalt Concrete Exposed to Moisture and Salt Erosion
Authors: Liang Yuheng , Chen Jiaqi , Li Liang
Published: March 2024

Salt erosion has an adverse impact on the durability of asphalt pavements. Porous asphalt concrete is particularly susceptible to the influence of salt. In this study, a finite element model was developed to investigate the fracture behavior of PAC exposed to salt erosion. The 2D heterogeneous structure of PAC was generated with an image-aided approach to computationally study the fracture behavior of PAC. Laboratory SCB tests were conducted to validate the finite element model. The simulation results of the SCB tests indicate that the peak load of the PAC decreased by 21.8% in dry-wet cycles and 26.1% in freeze-thaw cycles compared to the control group. The salt solution accelerated the degradation of the durability of PAC under both dry-wet cycles and freeze-thaw cycle conditions, which is consistent with laboratory tests. After flushing treatment before the drying phase, the peak load of the PAC in salt environments increased by 5.3% compared to that of the samples with no flushing. Salt erosion also results in a higher average value of scalar stiffness degradation (SDEG), and the damaged elements were primarily the cohesive elements in the fracture of the PAC. Additionally, the influence of crucial factors including the void content, adhesion and cohesion, and loading rate on the fracture behavior of the PAC was analyzed. As the void content increases, the average SDEG value of the cohesive elements increases and surpasses the average SDEG value of the adhesive elements at a void content of approximately 9%. The performance of the fine aggregate matrix (FAM) has a much greater impact than the FAM-aggregate interface on the durability of the PAC. And there were more damaged CZM elements with the increase in the loading rate. Salt erosion results in higher SDEG values and a larger number of cohesive damaged elements at each loading rate.
Auxetics and FEA: Modern Materials Driven by Modern Simulation Methods
Authors: Galea Mifsud Russell , Muscat Grace Anne , Grima-Cornish James N. , Dudek Krzysztof K. , Cardona Maria A. , Attard Daphne , Farrugia Pierre-Sandre , Gatt Ruben , Evans Kenneth E. , Grima Joseph N.
Published: March 2024

Auxetics are materials, metamaterials or structures which expand laterally in at least one cross-sectional plane when uniaxially stretched, that is, have a negative Poisson’s ratio. Over these last decades, these systems have been studied through various methods, including simulations through finite elements analysis (FEA). This simulation tool is playing an increasingly significant role in the study of materials and structures as a result of the availability of more advanced and user-friendly commercially available software and higher computational power at more reachable costs. This review shows how, in the last three decades, FEA proved to be an essential key tool for studying auxetics, their properties, potential uses and applications. It focuses on the use of FEA in recent years for the design and optimisation of auxetic systems, for the simulation of how they behave when subjected to uniaxial stretching or compression, typically with a focus on identifying the deformation mechanism which leads to auxetic behaviour, and/or, for the simulation of their characteristics and behaviour under different circumstances such as impacts.
Optimizing Thermoelectric Performance of Hybrid Crystals Bi2O2Se1−xTex in the Bi2O2X System
Authors: Xie Fan , Ma Zhiyao , Zhou Jian
Published: March 2024

In addressing the global need for sustainable energy conversion, this study presents a breakthrough in thermoelectric materials research by optimizing the Bi2O2Se1–xTex system in the Bi2O2Se/Bi2O2Te pseudobinary series. Leveraging the principles of innovative transport mechanisms and defect engineering, we introduce tellurium (Te) doping into Bi2O2Se to enhance its thermoelectric properties synergistically. With the help of various advanced characterization tools such as XRD, SEM, TEM, XPS, FTIR, TGA, LFA, and DSC, combined with relevant resistance and density measurement techniques, we conducted an in-depth exploration of the complex interactions between various factors within thermoelectric materials. We recognize that the balance and synergy of these factors in the thermoelectric conversion process are crucial to achieving efficient energy conversion. Through systematic research, we are committed to revealing the mechanisms of these interactions and providing a solid scientific foundation for the optimal design and performance enhancement of thermoelectric materials. Finally, the advantage coefficient (ZT) of the thermoelectric material has been significantly improved. The crystallographic analysis confirms the formation of a continuous series of mixed crystals with varying Te concentrations, adhering to Vegard’s law and exhibiting significant improvements in electrical and thermal conductivities. The Bi2O2Se1–xTex crystals, particularly the Bi2O2Se0.6Te0.4 composition, demonstrate a peak ZT of 0.86 at 373 K. This achievement aligns with recent advancements in defect-enabled mechanisms and band convergence and sets a new standard for high-performance thermoelectrics. The study’s findings contribute significantly to the ongoing quest for efficient thermal-to-electrical energy conversion, offering a promising avenue for future sustainable energy technologies.
SVD-Based Parameter Identification of Discrete-Time Stochastic Systems with Unknown Exogenous Inputs
Authors: Tsyganov Andrey , Tsyganova Yulia
Published: March 2024

This paper addresses the problem of parameter identification for discrete-time stochastic systems with unknown exogenous inputs. These systems form an important class of dynamic stochastic system models used to describe objects and processes under a high level of a priori uncertainty, when it is not possible to make any assumptions about the evolution of the unknown input signal or its statistical properties. The main purpose of this paper is to construct a new SVD-based modification of the existing Gillijns and De Moor filtering algorithm for linear discrete-time stochastic systems with unknown exogenous inputs. Using the theoretical results obtained, we demonstrate how this modified algorithm can be applied to solve the problem of parameter identification. The results of our numerical experiments conducted in MATLAB confirm the effectiveness of the SVD-based parameter identification method that was developed, under conditions of unknown exogenous inputs, compared to maximum likelihood parameter identification when exogenous inputs are known.
Efficient Maintenance of Minimum Spanning Trees in Dynamic Weighted Undirected Graphs
Authors: Luo Mao , Qin Huigang , Wu Xinyun , Xiong Caiquan , Xia Dahai , Ke Yuanzhi
Published: March 2024

This paper presents an algorithm for effectively maintaining the minimum spanning tree in dynamic weighted undirected graphs. The algorithm efficiently updates the minimum spanning tree when the underlying graph structure changes. By identifying the portion of the original tree that can be preserved in the updated tree, our algorithm avoids recalculating the minimum spanning tree from scratch. We provide proof of correctness for the proposed algorithm and analyze its time complexity. In general scenarios, the time complexity of our algorithm is comparable to that of Kruskal’s algorithm. However, the experimental results demonstrate that our algorithm outperforms the approach of recomputing the minimum spanning tree by using Kruskal’s algorithm, especially in medium- and large-scale dynamic graphs where the graph undergoes iterative changes.
Attenuation of Nicotine Effects on A549 Lung Cancer Cells by Synthetic α7 nAChR Antagonists APS7-2 and APS8-2
Authors: Joukhan Ahmad , Kononenko Veno , Bele Tadeja , Sollner Dolenc Marija , Peigneur Steve , Pinheiro-Junior Ernesto Lopes , Tytgat Jan , Turk Tom , Križaj Igor , Drobne Damjana
Published: March 2024

Nicotine binds to nicotinic acetylcholine receptors (nAChRs) that are overexpressed in different cancer cells, promoting tumor growth and resistance to chemotherapy. In this study, we aimed to investigate the potential of APS7-2 and APS8-2, synthetic analogs of a marine sponge toxin, to inhibit nicotine-mediated effects on A549 human lung cancer cells. Our electrophysiological measurements confirmed that APS7-2 and APS8-2 act as α7 nAChR antagonists. APS8-2 showed no cytotoxicity in A549 cells, while APS7-2 showed concentration-dependent cytotoxicity in A549 cells. The different cytotoxic responses of APS7-2 and APS8-2 emphasize the importance of the chemical structure in determining their cytotoxicity on cancer cells. Nicotine-mediated effects include increased cell viability and proliferation, elevated intracellular calcium levels, and reduced cisplatin-induced cytotoxicity and reactive oxygen species production (ROS) in A549 cells. These effects of nicotine were effectively attenuated by APS8-2, whereas APS7-2 was less effective. Our results suggest that APS8-2 is a promising new therapeutic agent in the chemotherapy of lung cancer.