bluuebunny/crossref_metadata_2025 huggingface dataset:
The Association between Physical Fitness Performance and Abdominal Obesity Risk among Taiwanese Adults: A Cross-Sectional Study
Authors: Lee Po-Fu , Ho Chien-Chang , Kan Nai-Wen , Yeh Ding-Peng , Chang Yun-Chi , Li Yu-Jui , Tseng Ching-Yu , Hsieh Xin-Yu , Chiu Chih-Hui
Published: March 2020

The present study aims to investigate the associations between physical fitness performance and abdominal obesity risk among Taiwanese adults. We conducted a cross-sectional study and reviewed the data derived from the National Physical Fitness Survey in Taiwan (HPFSIT). Data from a total of 62,486 respondents aged 23–64 years were collected in this study. The participants completed a standardized structural questionnaire and a series of anthropometric characteristics (body mass index and waist-to-hip ratio) and physical fitness (3-min step tests, 1-min sit-up tests, and sit-and-reach tests) assessments. Waist circumference was used to define abdominal obesity status. A multiple logistic regression analysis was conducted. Our results presented almost entirely significant associations (except for women) on the 3-min step test. Moreover, the results suggest that muscular strength, endurance, and flexibility may be effective predictors of abdominal obesity among men and women, whereas cardiorespiratory fitness predicted abdominal obesity only in men. According to the results of this study, the fitness–abdominal obesity associations are minor based on a mixed population analysis. However, dose–response relationships have been observed. The present study provides a new perspective by using different types of fitness performance to predict abdominal obesity.
Tissue Engineered Esophageal Patch by Mesenchymal Stromal Cells: Optimization of Electrospun Patch Engineering
Authors: Pisani Silvia , Croce Stefania , Chiesa Enrica , Dorati Rossella , Lenta Elisa , Genta Ida , Bruni Giovanna , Mauramati Simone , Benazzo Alberto , Cobianchi Lorenzo , Morbini Patrizia , Caliogna Laura , Benazzo Marco , Avanzini Maria Antonietta , Conti Bice
Published: March 2020

Aim of work was to locate a simple, reproducible protocol for uniform seeding and optimal cellularization of biodegradable patch minimizing the risk of structural damages of patch and its contamination in long-term culture. Two seeding procedures are exploited, namely static seeding procedures on biodegradable and biocompatible patches incubated as free floating (floating conditions) or supported by CellCrownTM insert (fixed conditions) and engineered by porcine bone marrow MSCs (p-MSCs). Scaffold prototypes having specific structural features with regard to pore size, pore orientation, porosity, and pore distribution were produced using two different techniques, such as temperature-induced precipitation method and electrospinning technology. The investigation on different prototypes allowed achieving several implementations in terms of cell distribution uniformity, seeding efficiency, and cellularization timing. The cell seeding protocol in stating conditions demonstrated to be the most suitable method, as these conditions successfully improved the cellularization of polymeric patches. Furthermore, the investigation provided interesting information on patches’ stability in physiological simulating experimental conditions. Considering the in vitro results, it can be stated that the in vitro protocol proposed for patches cellularization is suitable to achieve homogeneous and complete cellularizations of patch. Moreover, the protocol turned out to be simple, repeatable, and reproducible.
A Network-Based Approach to Explore the Mechanisms of Uncaria Alkaloids in Treating Hypertension and Alleviating Alzheimer’s Disease
Authors: Wu Wenyong , Zhang Zijia , Li Feifei , Deng Yanping , Lei Min , Long Huali , Hou Jinjun , Wu Wanying
Published: March 2020

Uncaria alkaloids are the major bioactive chemicals found in the Uncaria genus, which have a long history of clinical application in treating cardiovascular and mental diseases in traditional Chinese medicine (TCM). However, there are gaps in understanding the multiple targets, pathways, and biological activities of Uncaria alkaloids. By constructing the interactions among drug-targets-diseases, network pharmacology provides a systemic methodology and a novel perspective to present the intricate connections among drugs, potential targets, and related pathways. It is a valuable tool for studying TCM drugs with multiple indications, and how these multi-indication drugs are affected by complex interactions in the biological system. To better understand the mechanisms and targets of Uncaria alkaloids, we built an integrated analytical platform based on network pharmacology, including target prediction, protein–protein interaction (PPI) network, topology analysis, gene enrichment analysis, and molecular docking. Using this platform, we revealed the underlying mechanisms of Uncaria alkaloids’ anti-hypertensive effects and explored the possible application of Uncaria alkaloids in preventing Alzheimer’s disease. These results were further evaluated and refined using biological experiments. Our study provides a novel strategy for understanding the holistic pharmacology of TCM, as well as for exploring the multi-indication properties of TCM beyond its traditional applications.
Counteracting Radio-Resistance Using the Optimization of Radiotherapy
Authors: Chevalier François
Published: March 2020

Radiotherapy is an essential component of cancer therapy and remains one of the most (cost-) effective treatment options available [...]
The Arabidopsis GPI-Anchored LTPg5 Encoded by At3g22600 Has a Role in Resistance against a Diverse Range of Pathogens
Authors: Ali Muhammad Amjad , Abbas Amjad , Azeem Farrukh , Shahzadi Mahpara , Bohlmann Holger
Published: March 2020

Arabidopsis contains 34 genes for glycosylphosphatidylinositol (GPI)-anchored LTPg proteins. A motif analysis has placed these into four groups. With one exception, all are produced with a signal peptide and are most likely attached to the cell membrane via the GPI anchor. Several of the LTPg genes across the four groups are downregulated in syncytia induced by the beet cyst nematode Heterodera schachtii. We have here studied At3g22600 encoding LTPg5, which is the most strongly downregulated LTPg gene. It is mainly expressed in roots, and a promoter::GUS line was used to confirm the downregulation in syncytia and also showed downregulation in galls of the root knot nematode Meloidogyne incognita. In contrast, infection with bacteria (Pseudomonas syringae) and fungi (Botrytis cinerea) led to the induction of the gene in leaves. This diverse regulation of LTPg5 indicated a role in resistance, which we confirmed with overexpression lines and a T-DNA mutant. The overexpression lines were more resistant to both nematode species and to P. syringae and B. cinerea, while a knock-out mutant was more susceptible to H. schachtii and P. syringae. Thus, LTPg5 encoded by At3g22600 is part of the Arabidopsis resistance mechanism against pathogens. LTPg5 has probably no direct antimicrobial activity but could perhaps act by associating with a receptor-like kinase, leading to the induction of defense genes such as PR1.
The Complex Network between MYC Oncogene and microRNAs in Gastric Cancer: An Overview
Authors: Anauate Ana Carolina , Leal Mariana Ferreira , Calcagno Danielle Queiroz , Gigek Carolina Oliveira , Karia Bruno Takao Real , Wisnieski Fernanda , dos Santos Leonardo Caires , Chen Elizabeth Suchi , Burbano Rommel Rodríguez , Smith Marília Arruda Cardoso
Published: March 2020

Despite the advancements in cancer treatments, gastric cancer is still one of the leading causes of death worldwide. In this context, it is of great interest to discover new and more effective ways of treating this disease. Accumulated evidences have demonstrated the amplification of 8q24.21 region in gastric tumors. Furthermore, this is the region where the widely known MYC oncogene and different microRNAs are located. MYC deregulation is key in tumorigenesis in various types of tissues, once it is associated with cell proliferation, survival, and drug resistance. microRNAs are a class of noncoding RNAs that negatively regulate the protein translation, and which deregulation is related with gastric cancer development. However, little is understood about the interactions between microRNAs and MYC. Here, we overview the MYC role and its relationship with the microRNAs network in gastric cancer aiming to identify potential targets useful to be used in clinic, not only as biomarkers, but also as molecules for development of promising therapies.
Manipulation of Ascorbate Biosynthetic, Recycling, and Regulatory Pathways for Improved Abiotic Stress Tolerance in Plants
Authors: Broad Ronan C. , Bonneau Julien P. , Hellens Roger P. , Johnson Alexander A.T.
Published: March 2020

Abiotic stresses, such as drought, salinity, and extreme temperatures, are major limiting factors in global crop productivity and are predicted to be exacerbated by climate change. The overproduction of reactive oxygen species (ROS) is a common consequence of many abiotic stresses. Ascorbate, also known as vitamin C, is the most abundant water-soluble antioxidant in plant cells and can combat oxidative stress directly as a ROS scavenger, or through the ascorbate–glutathione cycle—a major antioxidant system in plant cells. Engineering crops with enhanced ascorbate concentrations therefore has the potential to promote broad abiotic stress tolerance. Three distinct strategies have been utilized to increase ascorbate concentrations in plants: (i) increased biosynthesis, (ii) enhanced recycling, or (iii) modulating regulatory factors. Here, we review the genetic pathways underlying ascorbate biosynthesis, recycling, and regulation in plants, including a summary of all metabolic engineering strategies utilized to date to increase ascorbate concentrations in model and crop species. We then highlight transgene-free strategies utilizing genome editing tools to increase ascorbate concentrations in crops, such as editing the highly conserved upstream open reading frame that controls translation of the GDP-L-galactose phosphorylase gene.
Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils
Authors: Skalickova Sylvie , Aulichova Tereza , Venusova Eva , Skladanka Jiri , Horky Pavel
Published: March 2020

It is generally believed that antibacterial essential oils have the potential to become one of the alternatives in preventing diarrheal diseases of monogastric animals. The disadvantage is their low efficiency per oral due to easy degradation during digestion in the stomach. This study compares the efficacy of chitosan, alginate-chitosan, guar gum-chitosan, xanthan gum-chitosan and pectin-chitosan nanocapsules to the synthesis of pH-responsive biopolymeric nanocapsule for Thymus vulgaris, Rosmarinus officinalis and Syzygium aromaticum essential oils. Using spectrophotometric approach and gas chromatography, release kinetics were determined in pH 3, 5.6 and 7.4. The growth rates of S. aureus and E. coli, as well as minimal inhibition concentration of essential oils were studied. The average encapsulation efficiency was 60%, and the loading efficiency was 70%. The size of the nanocapsules ranged from 100 nm to 500 nm. Results showed that chitosan-guar gum and chitosan-pectin nanocapsules released 30% of essential oils (EOs) at pH 3 and 80% at pH 7.4 during 3 h. Similar release kinetics were confirmed for thymol, eugenol and α-pinene. Minimal inhibition concentrations of Thymus vulgaris and Syzygium aromaticum essential oils ranged from 0.025 to 0.5%. Findings of this study suggest that the suitable pH-responsive nanocapsule for release, low toxicity and antibacterial activity is based on chitosan-guar gum structure.
Proregenerative Activity of IL-33 in Gastric Tissue Cells Undergoing Helicobacter Pylori-Induced Apoptosis
Authors: Gonciarz Weronika , Krupa Agnieszka , Chmiela Magdalena
Published: March 2020

Interleukin (IL)-33 is a proinflammatory mediator that alerts the host immune system to disorders in tissue homeostasis. Aim. To understand the role of IL-33 in modulating gastric tissue cell growth affected by Helicobacter pylori (H. pylori). Methods. IL-33 production in guinea pigs (Caviae porcellus) experimentally infected with H. pylori was evaluated by ELISA or immunohistochemical staining. The proregenerative activity of IL-33 was evaluated using gastric epithelial cells and fibroblasts that were naive or transfected with IL-33 siRNA exposed to H. pylori glycine acid extract antigenic complex (GE), as well as by measuring cell migration, proliferation, metabolic activity and apoptosis. Animals infected by H. pylori responded with increased production of IL-33. Also, cells treated in vitro with GE released more IL-33 than cells that were unstimulated. Silencing IL-33 in cells resulted in downregulation of metabolic activity, adhesion, migration and proliferation, especially after treatment with H. pylori GE, as well as upregulation of cells apoptosis associated with caspase 3 increase and Bcl-xL decrease, suggesting proregenerative activity of IL-33. Interestingly, upregulation of cell proliferation by IL-33 was Erk independent. Our results indicate that IL-33 may protect gastric tissue from loss of homeostasis caused by deleterious effects of H. pylori components and the inflammatory response developed during infection.
Genome-Wide Analysis of the Shi-Related Sequence Family and Functional Identification of GmSRS18 Involving in Drought and Salt Stresses in Soybean
Authors: Zhao Shu-Ping , Song Xin-Yuan , Guo Lin-Lin , Zhang Xiang-Zhan , Zheng Wei-Jun
Published: March 2020

The plant-special SHI-RELATED SEQUENCE (SRS) family plays vital roles in various biological processes. However, the genome-wide analysis and abiotic stress-related functions of this family were less reported in soybean. In this work, 21 members of soybean SRS family were identified, which were divided into three groups (Group I, II, and III). The chromosome location and gene structure were analyzed, which indicated that the members in the same group may have similar functions. The analysis of stress-related cis-elements showed that the SRS family may be involved in abiotic stress signaling pathway. The analysis of expression patterns in various tissues demonstrated that SRS family may play crucial roles in special tissue-dependent regulatory networks. The data based on soybean RNA sequencing (RNA-seq) and quantitative Real-Time PCR (qRT-PCR) proved that SRS genes were induced by drought, NaCl, and exogenous abscisic acid (ABA). GmSRS18 significantly induced by drought and NaCl was selected for further functional verification. GmSRS18, encoding a cell nuclear protein, could negatively regulate drought and salt resistance in transgenic Arabidopsis. It can affect stress-related physiological index, including chlorophyll, proline, and relative electrolyte leakage. Additionally, it inhibited the expression levels of stress-related marker genes. Taken together, these results provide valuable information for understanding the classification of soybean SRS transcription factors and indicates that SRS plays important roles in abiotic stress responses.
Global Characterization of GH10 Family Xylanase Genes in Rhizoctonia cerealis and Functional Analysis of Xylanase RcXYN1 During Fungus Infection in Wheat
Authors: Lu Lin , Liu Yongwei , Zhang Zengyan
Published: March 2020

Wheat (Triticum aestivum L.) is an important staple crop. Rhizoctonia cerealis is the causal agent of diseases that are devastating to cereal crops, including wheat. Xylanases play an important role in pathogenic infection, but little is known about xylanases in R. cerealis. Herein, we identified nine xylanase-encoding genes from the R. cerealis genome, named RcXYN1–RcXYN9, examined their expression patterns, and investigated the pathogenicity role of RcXYN1. RcXYN1–RcXYN9 proteins contain two conserved glutamate residues within the active motif in the glycoside hydrolase 10 (GH10) domain. Of them, RcXYN1–RcXYN4 are predicted to be secreted proteins. RcXYN1–RcXYN9 displayed different expression patterns during the infection process of wheat, and RcXYN1, RcXYN2, RcXYN5, and RcXYN9 were expressed highly across all the tested inoculation points. Functional dissection indicated that the RcXYN1 protein was able to induce necrosis/cell-death and H2O2 generation when infiltrated into wheat and Nicotiana benthamiana leaves. Furthermore, application of RcXYN1 protein followed by R. cerealis led to significantly higher levels of the disease in wheat leaves than application of the fungus alone. These results demonstrate that RcXYN1 acts as a pathogenicity factor during R. cerealis infection in wheat. This is the first investigation of xylanase genes in R. cerealis, providing novel insights into the pathogenesis mechanisms of R. cerealis.
Gibberellin Signaling Repressor LlDELLA1 Controls the Flower and Pod Development of Yellow Lupine (Lupinus luteus L.)
Authors: Marciniak Katarzyna , Przedniczek Krzysztof
Published: March 2020

Precise control of generative organ development is of great importance for the productivity of crop plants, including legumes. Gibberellins (GAs) play a key role in the regulation of flowering, and fruit setting and development. The major repressors of GA signaling are DELLA proteins. In this paper, the full-length cDNA of LlDELLA1 gene in yellow lupine (Lupinus luteus L.) was identified. Nuclear-located LlDELLA1 was clustered in a second phylogenetic group. Further analyses revealed the presence of all conserved motifs and domains required for the GA-dependent interaction with Gibberellin Insensitive Dwarf1 (GID1) receptor, and involved in the repression function of LlDELLA1. Studies on expression profiles have shown that fluctuating LlDELLA1 transcript level favors proper flower and pod development. Accumulation of LlDELLA1 mRNA slightly decreases from the flower bud stage to anther opening (dehiscence), while there is rapid increase during pollination, fertilization, as well as pod setting and early development. LlDELLA1 expression is downregulated during late pod development. The linkage of LlDELLA1 activity with cellular and tissue localization of gibberellic acid (GA3) offers a broader insight into the functioning of the GA pathway, dependent on the organ and developmental stage. Our analyses provide information that may be valuable in improving the agronomic properties of yellow lupine.
Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice?
Authors: Fan Xiaoru , Chen Jingguang , Wu Yufeng , Teo CheeHow , Xu Guohua , Fan Xiaorong
Published: March 2020

Transgenic technologies have been applied to a wide range of biological research. However, information on the potential epigenetic effects of transgenic technology is still lacking. Here, we show that the transgenic process can simultaneously induce both genetic and epigenetic changes in rice. We analyzed genetic, epigenetic, and phenotypic changes in plants subjected to tissue culture regeneration, using transgenic lines expressing the same coding sequence from two different promoters in transgenic lines of two rice cultivars: Wuyunjing7 (WYJ7) and Nipponbare (NP). We determined the expression of OsNAR2.1 in two overexpression lines generated from the two cultivars, and in the RNA interference (RNAi) OsNAR2.1 line in NP. DNA methylation analyses were performed on wild-type cultivars (WYJ7 and NP), regenerated lines (CK, T0 plants), segregation-derived wild-type from pOsNAR2.1-OsNAR2.1 (SDWT), pOsNAR2.1-OsNAR2.1, pUbi-OsNAR2.1, and RNAi lines. Interestingly, we observed global methylation decreased in the T0 regenerated line of WYJ7 (CK-WJY7) and pOsNAR2.1-OsNAR2.1 lines but increased in pUbi-OsNAR2.1 and RNAi lines of NP. Furthermore, the methylation pattern in SDWT returned to the WYJ7 level after four generations. Phenotypic changes were detected in all the generated lines except for SDWT. Global methylation was found to decrease by 13% in pOsNAR2.1-OsNAR2.1 with an increase in plant height of 4.69% compared with WYJ7, and increased by 18% in pUbi-OsNAR2.1 with an increase of 17.36% in plant height compared with NP. This suggests an absence of a necessary link between global methylation and the phenotype of transgenic plants with OsNAR2.1 gene over-expression. However, epigenetic changes can influence phenotype during tissue culture, as seen in the massive methylation in CK-WYJ7, T0 regenerated lines, resulting in decreased plant height compared with the wild-type, in the absence of a transformed gene. We conclude that in the transgenic lines the phenotype is mainly determined by the nature and function of the transgene after four generations of transformation, while the global epigenetic modification is dependent on the genetic background. Our research suggests an innovative insight in explaining the reason behind the occurrence of transgenic plants with random and undesirable phenotypes.
JA-Ile-Macrolactone 5b Induces Tea Plant (Camellia sinensis) Resistance to Both Herbivore Ectropis obliqua and Pathogen Colletotrichum camelliae
Authors: Lin Songbo , Dong Yanan , Li Xiwang , Xing Yuxian , Liu Miaomiao , Sun Xiaoling
Published: March 2020

Jasmonates (JAs), the group of lipid-derived hormones, were found to control the defense responses in a myriad of plants. Meaningfully, the macrolactones of 12-hydroxy jasmonate isoleucine (12OH-JA-Ile) were reported to induce the defensive response of wild tobacco. However, little to nothing has been known about the elicitation effect of JA-Ile-macrolactones on woody plants to harmful organisms, let alone its underlying mechanisms. Here, we first optimized the synthetic routine using mild toxic reagent isobutyl chloroformate instead of ethyl chloroformate for conjugation, and we used acetonitrile (MeCN) instead of ethyl alcohol for the better dissolution of p-toluenesulfonic acid (p-TsOH) to gain JA-Ile-macrolactones. JA-Ile-macrolactone 5b-treated tea plants significantly inhibited the larvae weight gain of Ectropis obliqua larvae and the lesions caused by Colletotrichum camelliae. Furthermore, the expression level of CsOPR3 was significantly upregulated in 5b-treated leaves. Meanwhile, 5b reduced the accumulation of eriodictyol 7-O-glucuronide (EDG) in tea plants, which was confirmed to promote the growth rate of E. obliqua larvae by artificial diet assay. In conclusion, our study proved that the exogenous application of 5b could induce the tea plant resistance both to herbivore E. obliqua and pathogen C. camelliae, and EDG was identified as one of the secondary metabolites that could influence the growth rate of E. obliqua, but it did not directly influence the infection of C. camelliae in vitro. Further research should be carried out to clarify the mechanism through which 5b induces tea plant resistance to C. camelliae.
Biological Activities and Molecular Docking of Brassinosteroids 24-Norcholane Type Analogs
Authors: Díaz Katy , Espinoza Luis , Carvajal Rodrigo , Conde-González Marcos , Niebla Vladimir , Olea Andrés F. , Coll Yamilet
Published: March 2020

The quest and design of new brassinosteroids analogs is a matter of current interest. Herein, the effect of short alkyl side chains and the configuration at C22 on the growth-promoting activity of a series of new brassinosteroid 24-norcholan-type analogs have been evaluated by the rice leaf inclination test using brassinolide as positive control. The highest activities were found for triol 3 with a C22(S) configuration and monobenzoylated derivatives. A docking study of these compounds into the active site of the Brassinosteroid Insensitive 1(BRI1)–ligand–BRI1-Associated Receptor Kinase 1 (BAK1) complex was performed using AutoDock Vina, and protein–ligand contacts were analyzed using LigPlot+. The results suggest that the hydrophobic interactions of ligands with the receptor BRI1LRR and hydrogen bonding with BAK1 in the complex are important for ligand recognition. For monobenzoylated derivatives, the absence of the hydrophobic end in the alkyl chain seems to be compensated by the benzoyl group. Thus, it would be interesting to determine if this result depends on the nature of the substituent group. Finally, mixtures of S/R triols 3/4 exhibit activities that are comparable or even better than those found for brassinolide. Thus, these compounds are potential candidates for application in agriculture to improve the growth and yield of plants against various types of biotic and abiotic stress.
Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells
Authors: Than Uyen Thi Trang , Le Huyen Thi , Hoang Diem Huong , Nguyen Xuan-Hung , Pham Cuong Thi , Bui Khanh Thi Van , Bui Hue Thi Hong , Nguyen Phong Van , Nguyen Tu Dac , Do Thu Thi Hoai , Chu Thao Thi , Bui Anh Viet , Nguyen Liem Thanh , Hoang Nhung Thi My
Published: March 2020

(1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells. (3) Results: We found that both lung tumor cell lysate-pulsed DCs and their exosomes could induce allogeneic T cell proliferation. Moreover, alloPBMCs primed with tumor cell lysate-pulsed DCs and their exosomes have a greater cytotoxic activity against A549 cells compared to unprimed cells and cells primed with unpulsed DCs and their exosomes. (4) Conclusion: Tumor cell lysate-pulsed DCs and their exosomes should be considered to develop into a novel immunotherapeutic strategy—e.g., vaccines—for patients with lung cancer. Our results also suggested that cryo umbilical cord blood mononuclear cells source, which is a readily and available source, is effective for generation of allogeneic DCs and their exosomes will be material for vaccinating against cancer.
Real-Time System for Driver Fatigue Detection Based on a Recurrent Neuronal Network
Authors: Ed-Doughmi Younes , Idrissi Najlae , Hbali Youssef
Published: March 2020

In recent years, the rise of car accident fatalities has grown significantly around the world. Hence, road security has become a global concern and a challenging problem that needs to be solved. The deaths caused by road accidents are still increasing and currently viewed as a significant general medical issue. The most recent developments have made in advancing knowledge and scientific capacities of vehicles, enabling them to see and examine street situations to counteract mishaps and secure travelers. Therefore, the analysis of driver’s behaviors on the road has become one of the leading research subjects in recent years, particularly drowsiness, as it grants the most elevated factor of mishaps and is the primary source of death on roads. This paper presents a way to analyze and anticipate driver drowsiness by applying a Recurrent Neural Network over a sequence frame driver’s face. We used a dataset to shape and approve our model and implemented repetitive neural network architecture multi-layer model-based 3D Convolutional Networks to detect driver drowsiness. After a training session, we obtained a promising accuracy that approaches a 92% acceptance rate, which made it possible to develop a real-time driver monitoring system to reduce road accidents.
Size of the Company as the Main Determinant of Talent Management in Slovakia
Authors: Savov Radovan , Lančarič Drahoslav , Kozáková Jana
Published: March 2020

Nowadays, all sources in the reproduction process are easily substituted, thus the most important factors in reaching a competitive advantage are human resources. Talent management is the process oriented to enrich higher the ability of employers to increase their quality and productivity. Globalization has changed the structure of the companies in Slovakia, depending on the size of the company. This paper compares how the size of the company influences the main phases of the talent management process (strategy, identification, assessment, development, retaining). A scaled questionnaire was applied as a tool for data collection in 381 companies operating business in Slovakia. Questionnaire reliability was verified by Cronbach’s alpha. To verify the existence of statistically significant differences between individual groups of respondents, ANOVA was used. We found that the main differences between small and large companies were identified in the phases of talent identification and talent development. In bigger companies, management is more focused on HR plans that include talent identification and acquisition and have more possibilities to develop talented individuals. On the other side we could see that small companies were more successful in the process of retaining the talents. Talented people in small companies are more loyal to the employers and stay in the company for longer periods than talented individuals in large companies.
Evolving Always-Critical Networks
Authors: Villani Marco , Magrì Salvatore , Roli Andrea , Serra Roberto
Published: March 2020

Living beings share several common features at the molecular level, but there are very few large-scale “operating principles” which hold for all (or almost all) organisms. However, biology is subject to a deluge of data, and as such, general concepts such as this would be extremely valuable. One interesting candidate is the “criticality” principle, which claims that biological evolution favors those dynamical regimes that are intermediaries between ordered and disordered states (i.e., “at the edge of chaos”). The reasons why this should be the case and experimental evidence are briefly discussed, observing that gene regulatory networks are indeed often found on, or close to, the critical boundaries. Therefore, assuming that criticality provides an edge, it is important to ascertain whether systems that are critical can further evolve while remaining critical. In order to explore the possibility of achieving such “always-critical” evolution, we resort to simulated evolution, by suitably modifying a genetic algorithm in such a way that the newly-generated individuals are constrained to be critical. It is then shown that these modified genetic algorithms can actually develop critical gene regulatory networks with two interesting (and quite different) features of biological significance, involving, in one case, the average gene activation values and, in the other case, the response to perturbations. These two cases suggest that it is often possible to evolve networks with interesting properties without losing the advantages of criticality. The evolved networks also show some interesting features which are discussed.
6-Imino-1,2,3,4,8,9,10,11-octahydropyrido[1,2-a]pyrido[1′,2′:1,2]imidazo[4,5-f]benzimidazole-13-one: Synthesis and Cytotoxicity Evaluation
Authors: Conboy Darren , Aldabbagh Fawaz
Published: March 2020

The first report of an iminoquinone of imidazo[4,5-f]benzimidazole is described. The 2D-NOESY spectrum of 1,2,3,4,8,9,10,11-octahydropyrido[1,2-a]pyrido[1’,2’:1,2]imidazo[4,5-f]benzimidazol-6-amine was used to confirm the location of the imine moiety at the C-6 position of the title compound. Cytotoxicity data from the National Cancer Institute are included.
Probabilistic Estimation of Fatigue Strength for Axial and Bending Loading in High-Cycle Fatigue
Authors: Tomaszewski Tomasz , Strzelecki Przemysław , Mazurkiewicz Adam , Musiał Janusz
Published: March 2020

In this paper, the sensitivity to the type of loads (axial and bending loading) of selected construction materials (AW6063 T6 aluminum alloy, S355J2+C structural steel, and 1.4301 acid-resistant steel) in high-cycle fatigue was verified. The obtained S-N fatigue characteristics were described by a probabilistic model of the 3-parameters Weibull cumulative distribution function. The main area of research concerned the correct implementation of the weakest link theory model. The theory is based on a highly-stressed surface area and a highly-stressed volume in the region of the highest stresses. For this purpose, an analytical model and a numerical model based on the finite element method were used. The model that gives the lowest error implemented in specific test conditions was determined on the basis of high-cycle fatigue analysis. For the analyzed materials, it was a highly-stressed volume model based on the weakest link theory.
Behaviour of Pre-Cracked Self-Healing Cementitious Materials under Static and Cyclic Loading
Authors: Anglani Giovanni , Tulliani Jean-Marc , Antonaci Paola
Published: March 2020

Capsule-based self-healing is increasingly being targeted as an effective way to improve the durability and sustainability of concrete infrastructures through the extension of their service life. Assessing the mechanical and durability behaviour of self-healing materials after damage and subsequent autonomous repair is essential to validate their possible use in real structures. In this study, self-healing mortars containing cementitious tubular capsules with a polyurethanic repairing agent were experimentally investigated. Their mechanical behaviour under both static and cyclic loading was analysed as a function of some factors related to the capsules themselves (production method, waterproof coating configuration, volume of repairing agent stored) or to the specimens (number, size and distribution of the capsules in the specimen). Their mechanical performances were quantified in terms of recovery of load-bearing capacity under static conditions and number of cycles to failure as a function of the peak force under cyclic conditions. Positive results were achieved, with a maximum load recovery index up to more than 40% and number of cycles to failure exceeding 10,000 in most cases, with peak force applied during cyclic loading at least corresponding to 70% of the estimated load-bearing capacity of the healed samples.
Optimizing Glass Fiber Molding Process Design by Reverse Warping
Authors: Chang Han-Jui , Su Zhi-Ming
Published: March 2020

The purpose of this study is to clarify the influence of changes in glass fiber properties on warpage prediction, and to demonstrate the importance of accurate material property data in the numerical simulation of injection molding. In addition, this study proposes an optimization method based on the reverse warping agent model, in which the thermal conductivity of the plastic material is reduced, and the solidified layer on the surface of the mold is reduced and transferred from the molding material to the mold wall. This means that by the end of the cooling phase, the shrinkage of the molten zone within the component will continue, resulting in warpage. Based on the optimal process parameters, the sensitivity of the warpage prediction to the relationship between the two most important material properties, the glass fiber and holding pressure time, was analyzed. The material property model constants used for numerical simulations can sometimes vary significantly due to inherent experimental measurement errors, the resolution of the test device, or the manner in which the curve fit is performed to determine the model constants. This model has been developed to intelligently determine the preferred processing parameters and to gradually correct the details of the molding conditions. Thus, the cavity is separated in the critical warpage region, and a new cavity geometry with a reverse warped profile is placed into the mold base slot. The results show that the hypothetical and reasonable variation of the glass fiber model constant and the holding pressure time relationship may significantly affect the magnitude of the warpage prediction of glass fiber products. The greatest differences were found as a result of the warping orientation of the glass fiber material.
Study on Wear and Fatigue Performance of Two Types of High-Speed Railway Wheel Materials at Different Ambient Temperatures
Authors: MA Lei , WANG Wenjian , GUO Jun , LIU Qiyue
Published: March 2020

The wear and fatigue behaviors of two newly developed types of high-speed railway wheel materials (named D1 and D2) were studied using the WR-1 wheel/rail rolling–sliding wear simulation device at high temperature (50 °C), room temperature (20 °C), and low temperature (−30 °C). The results showed that wear loss, surface hardening, and fatigue damage of the wheel and rail materials at high temperature (50 °C) and low temperature (−30 °C) were greater than at room temperature, showing the highest values at low temperature. With high Si and V content refining the pearlite lamellar spacing, D2 presented better resistance to wear and fatigue than D1. Generally, D2 wheel material appears more suitable for high-speed railway wheels.
The Distribution of Furfuryl Alcohol (FA) Resin in Bamboo Materials after Surface Furfurylation
Authors: Liu Minghui , Li Wanju , Wang Hankun , Zhang Xuexia , Yu Yan
Published: March 2020

In this study, bamboo was treated with an optimized surface furfurylation process. With this process, dimensionally stable and highly biologically durable bamboo material could be prepared without mechanical reduction. The anti-swelling efficiency (ASE) could reach 50% with a low weight percent gain (WPG about 13%). By using SEM, nanoindentation, and Imaging FTIR, we demonstrated that this high performance improvement is attributed to the unique furfuryl alcohol (FA) resin distribution pattern in the modified bamboo, namely a higher concentration of FA resin located in the region near to the surface of bamboo, and what is more, the preferred distribution of FA resin within the cell walls of parenchyma cells, which is known to be the weak point of bamboo both for biological durability and mechanical performances. Such graded modified bamboo could be utilized as a reliable engineering material for outdoor applications.
The Problem of Compressive Strength in Direction Perpendicular to the Grains on Example of Tests of the Load-Bearing Capacity of the Continuously Supported Timber-Frame Sill Plates
Authors: Brol Janusz , Kubica Jan , Węglorz Marek
Published: March 2020

This paper presents a discussion of the problem of compressive strength in a direction perpendicular to the grains based on test results of the joints made by timber posts and sill plate. These tests accompanied a larger series of full-scale tests of timber frame walls. The test elements were made of solid softwood (spruce). The wood moisture was low, which corresponds to the real working conditions of these elements in the walls of a building (low humidity is typical for dry wood in the built-in wall of a real building). In the tests, the compression strength of timber perpendicular to the grain was exceeded in the sill plate in the area in contact with the posts. Shortly before reaching the state of failure, large displacements in the sill plate were measured on the contact surface with the post, and the grains in the sill plates were cut off at the edge of the post. The full-scale test results showed an overestimation of the load-bearing capacity in compression perpendicular to the grain when calculated on the basis of EN 1995-1-1+A1:2008 (Eurocode 5), and, therefore, the need to modify the current approach for determining it.
Study of the Phase Transitions in the Binary System NPG-TRIS for Thermal Energy Storage Applications
Authors: Santos-Moreno Sergio , Doppiu Stefania , Lopez Gabriel A. , Marinova Nevena , Serrano Ángel , Silveira Elena , Palomo del Barrio Elena
Published: March 2020

Neopentylglycol (NPG) and tris(hydroxymethyl)aminomethane (TRIS) are promising phase change materials (PCMs) for thermal energy storage (TES) applications. These molecules undergo reversible solid-solid phase transitions that are characterized by high enthalpy changes. This work investigates the NPG-TRIS binary system as a way to extend the use of both compounds in TES, looking for mixtures that cover transition temperatures different from those of pure compounds. The phase diagram of NPG-TRIS system has been established by thermal analysis. It reveals the existence of two eutectoids and one peritectic invariants, whose main properties as PCMs (transition temperature, enthalpy of phase transition, specific heat and density) have been determined. Of all transitions, only the eutectoid at 392 K shows sufficiently high enthalpy of solid-solid phase transition (150–227 J/g) and transition temperature significantly different from that of the solid-state transitions of pure compounds (NPG: 313 K; TRIS: 407 K). Special attention has also been paid to the analysis of metastability issues that could limit the usability of NPG, TRIS and their mixtures as PCMs. It is proven that the addition of small amounts of expanded graphite microparticles contributes to reduce the subcooling phenomena that characterizes NPG and TRIS and solve the reversibility problems observed in NPG/TRIS mixtures.
A Novel Approach to Predict Wrinkling of Aluminum Alloy During Warm/Hot Sheet Hydroforming Based on an Improved Yoshida Buckling Test
Authors: Cai Gaoshen , Fu Jubo , Zhang Dongxing , Yang Jinlin , Yuan Yongfeng , Lang Lihui , Alexandrov Sergei
Published: March 2020

In order to predict the wrinkling of sheet metal under the influence of fluid pressure and temperature during warm/hot hydroforming, a numerical simulation model for sheet wrinkling prediction was established, taking into account through-thickness normal stress induced by fluid pressure. From simulations using linear and quadratic elements, respectively, it was found that the latter gave results that were much closer to experimental data. A novel experimental method based on an improved Yoshida Buckling Test (YBT) was proposed for testing the wrinkling properties of sheets under the through-thickness normal stress. A wrinkling coefficient suitable for predicting wrinkling was also presented. Based on the numerical simulations, an experimental validation of wrinkling performance was conducted. Ridge-height curves measured along the main diagonal tensile direction of the sheet were presented and showed that the wrinkling prediction criterion provided good discrimination. Furthermore, the wrinkling properties of several different materials were simulated to evaluate the accuracy of the prediction method, and the results revealed that the improved YBT gave good predictions for wrinkling in the conventional sheet metal forming process, while the prediction results for wrinkling in warm/hot sheet hydroforming were also accurate with the fluid pressure of zero.
Field Validation of Concrete Transport Property Measurement Methods
Authors: Abd El Fattah Ahmed , Al-Duais Ibrahim , Riding Kyle , Thomas Michael , Al-Dulaijan Salah , Al-Zahrani Mesfer
Published: March 2020

Reinforcing steel corrosion, caused by chloride ingress into concrete, is the leading cause of reinforced concrete deterioration. One of the main findings in the literature for reducing chloride ingress is the improvement of the durability characteristics of concrete by the addition of supplementary cementitious materials (SCMs) and/or chemical agents to concrete mixtures. In this study, standard ASTM tests—such as rapid chloride permeability (RCPT), bulk diffusion and sorptivity tests—were used to measure concrete properties such as porosity, sorptivity, salt diffusion, and permeability. Eight different mixtures, prepared with different SCMs and corrosion inhibitors, were tested. Apparent and effective chloride diffusion coefficients were calculated using bound chloride isotherms and time-dependent decrease in diffusion. Diffusion coefficients decreased with time, especially with the addition of SCMs and corrosion inhibitors. The apparent diffusion coefficient calculated using the error function was slightly lower than the effective diffusion coefficient; however, there was a linear trend between the two. The formation factor was found to correlate with the effective diffusion coefficient. The results of the laboratory tests were compared and benchmarked to their counterparts in the marine exposure site in the Arabian Gulf in order to identify laboratory key tests to predict concrete durability. The overall performance of concrete containing SCMs, especially fly ash, were the best among the other mixtures in the laboratory and the field.
Effect of Recycled Iron Powder as Fine Aggregate on the Mechanical, Durability, and High Temperature Behavior of Mortars
Authors: Miah Md Jihad , Ali Md Kawsar , Paul Suvash Chandra , John Babafemi Adewumi , Kong Sih Ying , Šavija Branko
Published: March 2020

This study evaluates the mechanical, durability, and residual compressive strength (after being exposed to 20, 120, 250, 400 and 600 °C) of mortar that uses recycled iron powder (RIP) as a fine aggregate. Within this context, mechanical strength, shrinkage, durability, and residual strength tests were performed on mortar made with seven different percentages (0%, 5%, 10%, 15%, 20%, 30% and 50%) of replacement of natural sand (NS) by RIP. It was found that the mechanical strength of mortar increased when replaced with up to 30% NS by RIP. In addition, the increase was 30% for compressive, 18% for tensile, and 47% for flexural strength at 28 days, respectively, compared to the reference mortar (mortar made with 100% NS). Shrinkage was observed for the mortar made with 100% NS, while both shrinkage and expansion occurred in the mortar made with RIP, especially for RIP higher than 5%. Furthermore, significantly lower porosity and capillary water absorption were observed for mortar made with up to 30% RIP, compared to that made with 100% NS, which decreased by 36% for porosity and 48% for water absorption. As the temperature increased, the strength decreased for all mixes, and the drop was more pronounced for the temperatures above 250 °C and 50% RIP. This study demonstrates that up to 30% RIP can be utilized as a fine aggregate in mortar due to its better mechanical and durability performances.
Corrosion Resistance of Heat-Treated Ni-W Alloy Coatings
Authors: Popczyk Magdalena , Kubisztal Julian , Swinarew Andrzej Szymon , Waśkiewicz Zbigniew , Stanula Arkadiusz , Knechtle Beat
Published: March 2020

The paper presents research on evaluation of corrosion resistance of Ni-W alloy coatings subjected to heat treatment. The corrosion resistance was tested in 5% NaCl solution by the use of potentiodynamic polarization technique and electrochemical impedance spectroscopy. Characteristics of the Ni-W coatings after heat treatment were carried out using scanning electron microscopy, scanning Kelvin probe technique and X-ray diffraction. Suggested reasons for the improvement of properties of the heat treated Ni-W coating, obtained at the lowest current density value (125 mA∙cm−2), are the highest tungsten content (c.a. 25 at.%) as well as the smallest and the most homogeneous electrochemically active surface area.