bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of pre-sowing seed treatment on seedlings growth rate and their excretory activity
Authors: Bozhkov A. I. , Kovalova M. K. , Azeez Z. A. , Goltvjansky А. V.
Published: May 2020

The importance of studying pre-sowing seed treatment lies in the possibility of regulating the rate of seed germination, the intensity of their growth and obtaining root exudates in biotechnology. The effect of three pre-sowing treatment methods was examined (control – washing with running water; the first method – washing with 0.05% sodium permanganate solution; the second method – 30 se­conds in 70% ethyl alcohol (C2H5OH) and 30 minutes in 5% sodium hypochlorite (NaOCl); the third method – 5 minutes in 70% C2H5OH and 40 minutes in 5% NaOCl) on the growth rate, germination rate, excretion rate of seeds of wheat and peas and composition (of protein, carbohydrate, amino acid content) of root exudates from the first to the third day of growth in order to obtain root exudates. It was revealed that the same pre-sowing treatment of wheat and pea seeds has a different effect on the rate and variability of seedling growth from the first to the third day, as well as on the qualitative and quantitative composition of root exudates. It was shown that pre-sowing treatment of wheat and pea seeds for 5 minutes with 70% ethanol followed by treatment with sodium hypochlorite (a “hard” treatment method) accelerates seedling growth and seed germination. This method of treatment reduces the intensity of excretion of root exudates and composition in wheat, but it increases the intensity of excretion in peas. The discovered effects can be explained by hormesis. Additionally, the third method of pre-sowing seed treatment can be used in root technologies for obtaining root exudates.
Influence of modern treatment regimens on serum biochemical parameters in piglets with gastroenteritis
Authors: Lukashchuk B. O. , Slivinska L. G. , Shcherbatyy A. R. , Zinko H. O. , Gutyj B. V.
Published: May 2020

The article presents analyses of biochemical parameters of blood serum in weaned piglets with non-contagious gastroenteritis and after their treatment with a probiotic (live spores of Bacillus cereus var. toyoi; silicic acid; calcium carbonate) and a phytobiotic (natural extracts of Oreganum vulgare, Cinnamomun cassia and Capsicum annuum; hydrogenated rapeseed oil) as part of the basic ration in combination with antibiotic (10% solution of enrofloxacin hydrochloride) on a modern pig farm. In animals of the experimental groups with gastroenteritis we established hypoproteinemia, hypoalbuminemia, hypoglycemia, hypoureaemia, hypercreatininemia, hyperbilirubinemia and increase of enzyme activity compared to parameters of clinically healthy piglets. The study established that the use of probiotics and phytobiotics for weaned piglets had a positive influence on protein synthesis function of the liver, as indicated by the normalization of serum total protein and albumins. Also, we found a positive influence of probiotics and phytobiotics on intensity of protein metabolism, as indicated by an increase of serum urea to the level of clinically healthy piglets. Use of phytobiotics for piglets had a positive influence on the process of gluconeogenesis in their body, which is indicated by the normalization of serum glucose to the level of clinically healthy piglets. Also, the use of probiotics and phytobiotics had a positive influence on the pigment-forming function of the liver, as indicated by the reduction of serum total bilirubin to the level of clinically healthy piglets. The use of probiotics and phytobiotics reduced activity of serum alanine and aspartate aminotransferases in the piglets of the experimental groups, indicating the stabilization of hepatocytes’ cell structures (mitochondrial and cytosolic). The study established positive influence of probiotics and phytobiotics on the functioning of the liver and biliary tract, as indicated by decreased activity of serum alkaline phosphatase and gamaglutamiltranspeptidase to the level of clinically healthy piglets. So, addition of probiotics and phytobiotics to mixed fodder up to 45 days of age, normalizes functioning of the kidneys and liver in weaned piglets with gastroenteritis.
Peculiarities of leukocyte apoptosis modulation in children with pyelonephritis
Authors: Marchenko I. А. , Babiichuk L. O. , Mishyna M. M. , Makieieva N. I. , Zubov P. M.
Published: May 2020

One of the leading places among inflammatory diseases of the urinary tract of children belongs to pyelonephritis, the course of which presents in most cases as a severe infectious disease threatening the patient’s life, which is the main reason for development of chronic kidney failure. This study was conducted to compare apoptosis stages in peripheral blood of children of different age categories with pyelonephritis depending on etiological factor and complications. The problem of mechanisms underlying immune system misregulation, especially functional activity of leukocytes in children with pyelonephritis, have not been explored in recent years. Assessment of leukocytes (neutrophils) apoptosis stages in peripheral blood of children of different age categories with pyelonephritis depending on complications and etiological factor was the aim of present study. The children's peripheral blood samples were analysed and assessed using a flow cytofluorimeter. The present study demonstrates an increase of the level of apoptotic cells at an early stage of apoptosis in children of all age categories with chronic pyelonephritis, which can be explained by associations of a wide range of pathogens and the presence of sequelae. An increase in the number of apoptotic cells in the late stage of apoptosis is observed in children aged 1 month – 8 years, in children 8–18 years, the amount of apoptotic cells is reduced by 1.5 times. The study of apoptosis stages allows complete characterization of the dynamics of the apoptotic process and supplementation of the pathogenesis of pyelonephritis in children. Such studies will make it possible to affect apoptosis modulation to regulate or correct it and encourage the finding of innovative solutions in the treatment related to influence on the immune response. We conclude that enhancement of peripheral blood leukocyte apoptosis in chronic form of pyelonephritis especially in young children is due to the polyetiology of this form of pyelonephritis and the development of complications.
Effect of L-arginine and aminoguanidine on the cytokine profile in obstetric antiphospholipid syndrome
Authors: Yaremchuk O. Z. , Posokhova K. A. , Lykhatskyi P. H. , Letniak N. Y. , Moseychuk I. P.
Published: May 2020

Antiphospholipid syndrome (APS) is one of the autoimmune causes of miscarriage in pregnancy. We researched the influence of L-arginine (25 mg/kg), the precursor of nitric oxide synthesis, and aminoguanidine, the inhibitor of inducible NO-synthase (10 mg/kg) on the concentration of IL-1β, IL-6, TNF-α, IL-4, IL-10 in serum of BALB/c mice on the 18th day of pregnancy in cases of APS. In the serum of the pregnant mice with APS, an increase in the concentration of proinflammatory cytokines (IL-1β, IL-6, TNF-α) and a decrease in the concentration of anti-inflammatory cytokines (IL-4 and IL-10) was evidenced, relative to those of the pregnant animals without APS. With the use of L-arginine, a decrease in the concentration of IL-1β, IL-6, TNF-α and an increase in the concentration of IL-4 and IL-10 was established, compared with the indicators of the pregnant mice with APS. The introduction of aminoguanidine, the selective inhibitor of iNOS, did not cause any changes in IL-1β, IL-6 and IL-10 compared with the group of animals with APS. However, a decrease in TNF-α concentration and an increase in IL-4 concentration were proved in this series compared with the group of pregnant animals with APS. Combined administration of L-arginine and aminoguanidine caused a decrease in the concentration of IL-1β, IL-6, TNF-α and an increase in the concentration of IL-4 and IL-10, compared with the indicators of the animals with APS. Thus, the combined administration of L-arginine and aminoguanidine to the pregnant mice with APS contributes to the rebalancing of proinflammatory and anti-inflammatory cytokines in the serum more than their individual use.
QUANTITATIVE MORPHO-FUNCTIONAL ANALYSIS OF SIMMETRY-ASIMMETRY CONDITION OF HUMAN NORMAL KIDNEY
Authors: Dgebuadze M. , Svanidze G. , Gachechiladze D.
Published: May 2020

The aim of the present research was to perform quantitative morpho-functional analysis of the symmetry-asymmetry condition of human normal autopsy and “live” kidneys using the complex of morphological and ultra-sonographic methods. METHODS: Morphometric study of 10 autopsy kidneys of healthy men 36 - 60 years of age was conducted; retrospective analysis of data obtained by vital ultra-sonographic study of 65 kidneys of men without renal diseases at the same age period was performed as well. All kidneys were with a single renal artery. RESULTS: The kidney length and width are statistically significantly greater on right, than on left, but there were no statistically significant differences between right and left kidneys in thickness of the kidney and size of its parenchyma. Results of morphometric study of autopsy renal glomeruli, as well as multislice computed tomographic angiography and doppler investigation of renal artery in color duplex scan mode did not show any statistically significant differences on right and on left. CONCLUSIONS: For determining of the standard quantitative indicators of kidney it is necessary to take into account the age and gender of the studied people, as well as the effect of side.
SCREENING CAPSICUM GENOTYPES FOR INCREASED DROUGHT TOLERANCE BY IN VITRO POLLEN GERMINATION AND
POLLEN TUBE LENGTH
Authors: Arnaoudova Y. , Arnaoudov B.
Published: May 2020

Yield realizing in vegetable crops depends on several reproductive processes that include pollen fertility as well as pollen tubes germination and growth. The study was conducted as the pollen characteristics fertility and viability were used, as well as the productivity for evaluation of drought tolerance in four pepper genotypes Capsicum annuum L. From the conducted cytological analyses was found that decreasing the water standard with 50% exert negative influence over the male gametophyte and the pollen fertility decreases with up to 37.4% average. More sensitive to drought showed to be the plants from both cultivars Pazardzhishka kapia and Albena, in which lower productivity and lower restorable potential were found in comparison with those found in the mutant lines No.1936 and No.1917. Increased tolerance showed the male gametophyte of line No.1936. After a drought in it were found the highest values for pollen viability – 33.4% average pollen germination and 438.7µm pollen tubes length which was with proven mathematical difference compared with the rest of the genotypes. These results showed that pollen fertility analyses and pollen viability analyses in drought plants could be used for evaluation of the drought tolerance.
A társas helyzetben tapasztalt flow-élmény kapcsolata az élettel való elégedettséggel és a pszichológiai jólléttel, fiatal felnőtteknél
Authors: Tímea Magyaródi , Attila Oláh
Published: May 2020

Elméleti háttér: A flow a jóllét növelésének egyik eszköze is lehet, aktivitáselméletként a viselkedés pozitív következményeire, növekedéshez való hozzájárulására épít. Cél: A társas helyzetben tapasztalt áramlat-élmény összefüggéseit a jóllét különböző dimenzióival – élettel való elégedettség és pszichológiai jóllét – ezidáig nem vizsgálták, holott a társas kapcsolatok jóllétet növelő szerepét több koncepció hangsúlyozza. Jelen kutatás célja ezen összefüggés igazolása. A társas flow gyakoriságát, intenzitását, valamint a flow szinkronizáció tényezőinek hatását vizsgáljuk. Módszerek: 1060 fő, 18 éven felüli vizsgálati személy, életkor: M(SD) = 26,67(10,76) év, vett részt az online kérdőíves módszerrel lebonyolított keresztmetszeti kutatásunkban. A kérdőíveket anonim módon töltötték ki: Általános Flow Leírás, Általános Flow Leírás Társas Interakciókban, Flow Állapot Kérdőív, Flow Szinkronizáció Kérdőív, Élettel Való Elégedettség Skála és Pszichológiai Jóllét Skálák. Eredmények: A hierarchikus lineáris regresszióanalízis alapján elmondható, hogy a társas helyzetben átélt flow-élmény gyakorisága (ß = 0,07; p = 0,038) és intenzitása (ß = 0,08; p = 0,039) az élettel való elégedettség szignifikáns, kismértékű magyarázó tényezője. Az egyéni helyzetben átélt flow gyakorisága (ß = 0,10; p = 0,002) és a társas helyzetben tapasztalt optimális élményhez kapcsolódó szinkronizációs tényezők (az együttműködő, flow-t indukáló helyzet interakciós jellemzői) (ß = 0,15; p < 0,001) szintén szignifikáns meghatározói a pszichológiai jóllétnek. Köuetkeztetések: Eredményeink alapján valószínűsíthető, hogy a flow-élmény társas helyzetben hozzájárul a jóllét különböző aspektusaihoz, átélési gyakorisága, intenzitása, valamint az interakcióból fakadó szinkronizációs dimenziók támogatják a jóllét magasabb szintjének elérését.
Az ártalmas gyermekkori élmények hatása a felnőttkori egészségi állapotra – szakirodalmi összefoglaló
Authors: Anikó Ujhelyiné Nagy , Ildikó Kuritárné Szabó
Published: May 2020

A gyermekkorban (családon belül) elszenvedett traumatizáció fontos, de sokszor fel nem ismert patogén tényező, amely az egész személyiségfejlődést (de)formálhatja, és szerepet játszik nem csupán a későbbi pszichológiai károsodások, hanem az egészségkárosító viselkedésformák, valamint a krónikus egészségi problémák kialakulásában is. Míg a gyermekkori ártalmas élmények szerepe a mentális betegségek etiológiájában régóta ismert, addig a kapcsolata az akár élethosszig jelenlevő, krónikus szomatikus problémákkal és megbetegedésekkel csupán az utóbbi évtizedekben került a nemzetközi tudományos érdeklődés középpontjába. A gyermekkorban elszenvedett krónikus traumatikus élmények sokrétű neurobiológiai, hormonális és immunműködésbeli eltéréseket eredményeznek, amelyek mediáló tényezőkként közvetítik az ártalom patogén hatását számos szervrendszer irányába. A nemzetközi kutatások eredményei bizonyították ezen élményeknek a felnőttkori egészségkárosodásban játszott oki szerepét, valamint azt is, hogy egy kellően fel nem ismert „járványról“ beszélhetünk, amennyiben a gyermekkori traumatizáció elterjedtségét és az abból következő kedvezőtlen következményeket tekintjük. Célunk az öszszefoglaló során áttekintést nyújtani a nemzetközi szakirodalomban fellelhető főbb kutatási eredményekről, amelyek összegzik eddigi tudásunkat az ártalmas gyermekkori élmények szomatikus egészségi állapotra gyakorolt hatásáról. Említést teszünk többek között a daganatos és kardiovaszkuláris megbetegedésekkel, egészségkárosító magatartásformákkal, emésztő- és légzőrendszerrel, krónikus fájdalomszindrómával, alvászavarokkal és korai halállal kapcsolatban feltárt összefüggésekről. Mindez hozzájárul a korai pszichoszociális rizikófaktorok elterjedtségének és felnőttkorra is átívelő súlyos következményeinek felismeréséhez, és felhívja a figyelmet a gyermekkori ártalmas élmények megelőzésének és kezelésének népegészségügyi jelentőségére.
Range extension of Mesophyllum erubescens (Foslie) Me. Lemoine (Hapalidiales, Rhodophyta): first report from mesophotic rhodolith beds in the northwestern Gulf of Mexico offshore Louisiana and Texas, including the Flower Garden Banks National Marine Sanctuary
Authors: Richards Joseph L. , Kittle III Ronald P. , Abshire Jaida R. , Fuselier Dijel , Schmidt William E. , Gurgel Carlos F. D. , Fredericq Suzanne
Published: May 2020

DNA sequence analysis of plastid-encoded psbA and UPA, mitochondrion-encoded COI, and nuclear-encoded LSU rDNA of rhodolith-forming crustose coralline algal specimens from the northwestern Gulf of Mexico reveals that Mesophyllum erubescens (Foslie) Me. Lemoine is present in mesophotic rhodolith beds offshore Louisiana and Texas at 39–57 m depth. Morpho-anatomical characters viewed with SEM support the identification of these specimens. Mesophyllum erubescens is reported for the first time offshore Louisiana at Ewing Bank, the Louisiana–Texas border at Bright Bank, and Texas in the Flower Garden Banks National Marine Sanctuary.
Tinjauan Hukum Islam terhadap Implikasi Fenomena Kawin Kontrak dalam Kehidupan Sosial, Ekonomi Masyarakat Desa
Authors: Arifin Muhamad Ramdan
Published: May 2020

Fenomena kawin kontrak yang marak terjadi di kawasan Desa Tugu Selatan Cisarua Bogor, Keabsahan suatu perkawinan didasarkan pada pemenuhan rukun dan syarat perkawinan. Rukun perkawinan dibahas Kompilasi Hukum Islam,sedangkan syarat perkawinan dimuat dalam Kompilasi Hukum Islam dan Undang-Undang Nomor 1 tahun 1974 tentang Perkawinan. Dalam Hukum  Islam dikenal nikah mut’ah, Keabsahan nikah mut’ah tersebut merupakan masalah khilafiyah antara Sunni dan Syiah sampai saat ini. Artikel ini untuk menganalisis pelaksanaan fenomena kawin kontrak serta faktor-faktor yang melatar belakanginya, dan juga menganalisis peranan tokoh masyarakat serta aparatur pemerintah dalam menanggulangi fenomena tersebut serta tinjauan hukum Islam terhadap implikasi fenomena kawin kontrak dalam kehidupan masyarakat baik secara sosial dan ekonomi yang dapat menimbulkan akibat hukum. Metode Pengumpulan data yang digunakan melalui pengumpulan dokumen, observasi dan wawancara Melalui pendekatan kualitatif. Akhirnya penelitian ini menemukan bahwa praktek kawin kontrak yang berlangsung tidak sesuai dengan ketentuan nikah mut’ah juga undang-undang perkawinan
Metode Diskusi Lembaga Kajian dan Pengembangan Sumberdaya Manusia Nahdlatul Ulama
Authors: Ghani Agi M. Abdul
Published: May 2020

Kegiatan diskusi merupakan suatu metode dakwah yang tercantum dalam al-Qur’an yang selama ini cenderung kurang populer. Lakpesdam NU adalah perangkat ormas NU yang berfungsi sebagai lembaga kajian isu-isu strategis dan pemberdayaan manusia untuk transformasi sosial yang berkeadilan dan bermartabat. Metode kajian diskusi Lakpesdam PCNU kota Tasikmalaya terkait dengan keislaman, menekankan aspek rasionalitas, argumentasi, dan aspek penunjang diskusi lainnya. Pendekatan terhadap masalah diatas menggunakan pendekatan teori metode dakwah diskusi dengan jenis penelitian kualitatif yaitu menggunakan paradigma interpretif. Teknik pengumpulan data dalam penelitian ini menggunakan teknik observasi, wawancara mendalam, dan dokumentasi. Adapun analisis data yang dilakukan dalam penelitian ini dilakukan dengan beberapa tahap, yakni tahap identifikasi data, kategorisasi data, reduksi data, seleksi data, analisis data, dan penarikan kesimpulan. Hasil penelitian menunjukkan bahwa, teknis kegiatan diskusi yang dilakukan oleh Lakpesdam PCNU kota Tasikmalaya dilakukan secara bebas dan santai, namun tetap pada koridor pertukaran pemikiran yang serius, serta berjalan secara demokratis. Orang-orang yang terlibat dalam kegiatan diskusi tersebut, mengemukakan pendapatnya secara argumentatif dengan menggunakan buku-buku kontemporer ataupun kitab-kitab klasik.
Gerakan Dialog Keagamaan Jaringan Kerja Antar Umat Beragama (Jakatarub) Dan Kontribusinya Terhadap Kerukunan Hidup Umat Beragama
Authors: Wahyuni Dwi
Published: May 2020

Merupakan penelitian mengenai gerakan dialog keagamaan Jaringan Kerja Antar Umat Beragama (JAKATARUB) dan kontribusinya terhadap kerukunan hidup umat beragama di Kota Bandung. Tujuan dari penelitian ini untuk menjelaskan strategi dan taktik yang dilakukan aktivis gerakan dialog keagamaan JAKATARUB, menganalisis faktor-faktor pendukung dan penghambat gerakan dialog keagamaan JAKATARUB dan mengetahui kontribusi gerakan dialog keagamaan JAKATARUB terhadap kerukunan hidup umat beragama di Kota Bandung.  Menggunakan metode kualitatif, dengan teknik pengumpulan data secara observasi, wawancara, dokumentasi dan studi kepustakaan. Kerangka teori yang digunakan adalah teori mobilisasi sumberdaya dan teori proses framing. JAKATARUB dalam gerakan dialog keagamaannya ialah memperluas jaringan, mobilisasi sumberdaya dan merubah struktur organisasi. Selanjutnya strategi JAKATARUB, diturunkan kepada bagian-bagian kecil dari setiap langkah gerakan. Bagian-bagian tersebut ialah pelaksanaan program-program yang bekerja sama dengan mitra JAKATARUB dan menyampaikan makna dari isu-isu prioritas gerakan kepada kelompok sasaran. Pada pelaksanaan program-program dan penyampaian makna merupakan taktik dari gerakan dialog keagamaan JAKATARUB.
CARACTERIZAÇÃO EPIDEMIOLÓGICA DOS CASOS DE COVID-19 NO MARANHÃO: Uma breve análise
Authors: Almeida Joelson dos Santos , Cardoso Jonas Alves , Cordeiro Eduardo Costa , Lemos Messias , Araújo Telma Maria Evangelista de , Sardinha Ana Hélia de Lima
Published: May 2020

Introdução: O COVID-19 (SARS-CoV-2) é um vírus que pode causar complicações respiratórias de leve a grave e elevada infectividade e patogenicidade. Atualmente, dado a sua alta transmissibilidade e dificuldade de controle, é considerado um evento pandêmico pelaOrganização Mundial da Saúde (OMS). O objetivo do estudo foi analisar o perfil epidemiológico de notificações de casos do novo coronavírus (SARS-Cov-2) no Estado do Maranhão. Delineamento: Trata-se de um levantamento epidemiológico dos casos e óbitos notificados de Covid-19 no estado do Maranhão no período de março a abril de 2020. Os dados foram analisados conforme informes do boletim epidemiológico de saúde do Estado do Maranhão com informações das regionais de saúde do estado. Os casos foram analisados por meio das variáveis: sexo (feminino/masculino), faixa etária (em anos), procedência, presença ou não de comorbidades. Resultados: Foram confirmados 2.105 casos de COVID-19. A região mais acometida foi São Luís com 89% dos casos sendo do sexo feminino (52%), e idade de 30 a 39 anos (29%). O sexo masculino apresentou maior taxa de óbitos (62%), idade 60 anos ou mais (66%), procedência regional de São Luís 60%, comorbidades diversas (78%), destacando-se as doenças cardiovasculares e imunológicas. Implicações: Observam-se impactos clínicos, epidemiológicos, econômicos, além de sérias repercussões sociais, políticas e culturais na nossa sociedade, implicando na capacidade de respostas rápidas aos desafios colocados pela pandemia.
Terapia ocupacional em tempos de pandemia: Seguridade social e garantias de um cotidiano possível para todos
Authors: Malfitano Ana , Cruz Daniel , Lopes Roseli
Published: May 2020

N/A
Significant Benefits of Osimertinib Against Adenosquamous Carcinoma Harboring Germline T790M Mutation
Authors: Huo Ran , Li Jinghua , Li Xiaofang , Shi Junping , Wang Kunjie , Jiao Jin , Shang Yanhong
Published: May 2020

Background

The identification of epidermal growth factor receptor (EGFR) mutations represents a milestone in the treatment of advanced non-small cell lung cancer. Patients with lung adenosquamous carcinomas (ASCs) rarely present with germline EGFR T790M mutation. The optimal treatment for cancers with germline EGFR T790M mutation (especially ASC) is not clear.

Materials and Methods

Using next-generation sequencing, we tested 450 cancer-related genes in a 27-year-old patient's lung adenosquamous carcinoma and matched blood samples. Germline mutations in samples from the patient's available relatives were identified by Sanger sequencing.

Results

We identified germline EGFR T790M mutation in the patient's lung adenosquamous carcinoma. He was treated with osimertinib and achieved complete response for more than 30 months, without significant drug-related adverse events. Genetic testing showed that germline EGFR T790M mutation might be a characteristic of inherited lung cancer.

Conclusion

Osimertinib can be a treatment option for patients with lung ASC harboring germline EGFR T790M mutation.

Key Points

A patient with adenosquamous carcinoma harboring a germline T790M mutation responded well to osimertinib with a progression-free survival of more than 30 months and without any unexpected toxicities. Osimertinib is effective for patients with lung squamous cell carcinoma with T790M and L858R mutations. The germline EGFR T790M mutation is associated with genetic susceptibility to lung cancer. The clinical use of next-generation sequencing could maximize the benefits of precision medicine in patients with cancer.
Basophil Count, Percentage of Basophil and Basophil Lymphocyte Ratio in Psychiatry Practice: Current Approaches and Future Directions
Authors: ORUM Mehmet Hamdi
Published: May 2020

It is an easy and inexpensive method to
utilize hematologic parameters in cases where we cannot get clear information
from the patients. The basophils are definitely involved in immunopathologic
responses to parasites, and in this respect, there may be a relationship between parasitic
infections and developmental disorders. In this study, we discussed the possible uses of basophil and related parameters.
Evaluation Of Demographic Characteristics And Clinical Outcomes Of Patients Presenting To The Emergency Department With Suicide Attempt
Authors: ATİK Dilek , YALIMOL Nusin , BULUT Bensu , ERDUR Ahmet , KAYA Hilmi , UNAL Ramazan , GUVEN Ramazan , CANDER Basar
Published: May 2020

Objectives:Suicide
is a state of aggression that is voluntarily directed towards the self. Suicide
cases with increasing mortality and morbidity rates with each passing day are
an important public health problem. In this study, it
was aimed to evaluate the demographic characteristics, methods of suicide
attempt with variable factors of clinical results of the patients who applied
with the reason of suicide attempt, and to contribute to literature with the
results. Methods:
This study was performed retrospectively by examining forensic records and
patient files of patients who came to a Training and Research Hospital
Emergency Medicine Clinic with the reason of suicide between the dates of
01.12.2018-31.12.2018. Chi-Square Independence,
Chi-Square Trend, and Fisher's Exact tests were used in statistical. p
<0.05 was considered statistically significant.Results:
A total of 504 patients were included in our study.Among the participants,
70.4%  were female and 29.6% were male.
The mean age of the participants was 30.14. Education levels were evaluated, 67.7%
were high school graduates, 22.4% university graduates, 8.1% middle school
graduates and 1.8% elementary school graduates.The
suicide attempt months between groups was not statistically significant in the
presence of psychiatric disorder Glaskow coma scale, final clinical results,
distribution of suicide attempt according to months and the time of suicide
attempt was found to be statistically significant .

Conclusion: The phenomenon of suicide is a situation that has not lost its
importance on the agenda both in our country and the world for centuries as one
of the causes of early death that can be prevented,unlike normal life
expectancy.We believe that individuals should be, in addition to check-ups
starting in primary school, periodically checked for mental health starting
from school age, and that possible suicide attempts can be prevented by working
together with psychological counselors starting from school age.
nCoV-2019 sequencing protocol (RAPID barcoding, 1200bp amplicon) v1
Authors: Freed Nikki , Silander Olin
Published: May 2020

To enable faster, easier sequencing of SARS-COV2 genomes with fewer steps than current methods, we use multiplexed 1200 base pair PCR amplicons with the Oxford Nanopore RAPID barcoding kit (RBK004). This is a modification of the ARTIC amplicon V3 sequencing protocol for MinION for nCoV-2019 developed by Josh Quick, which produces 400 base pair amplicons and uses the Oxford Nanopore Ligation barcoding kit (LSK-109). We have increased the size of the amplicons to 1200bp and use the RAPID barcode kit (RBK004), which enables requires less time and fewer reagents than the LSK-109 protocol. The amplicons produced in this protocol could also be used for Illumina sequencing. Primers were all designed using Primal Scheme: http://primal.zibraproject.org/, described here https://www.nature.com/articles/nprot.2017.066. Primer sequences are here: https://docs.google.com/spreadsheets/d/1M5I_C56ZC8_2Ycgm9EFieVlVNqxsP7dXAnGoBZy3nDo/edit?usp=sharing We can ship a small amount of pooled primers to interested labs for further testing, email freednikki@gmail.com or olinsilander@gmail.com
Long reads nanopore sequencing to recover SARS-CoV-2 whole genome v2
Authors: Resende Paola
Published: May 2020

This protocol describes step-by-step instructions for building long (~2kb) amplicon libraries to recover SARS-CoV-2 genomes using the nanopore sequencing. It can be applied for sequencing on the MinION device or GridION. This protocol was developed in collaboration with the Laboratory of Respiratory Viruses and Measles, Oswaldo Cruz Institute, FIOCRUZ, Brazil, and the two sequencing facilities at Pathogen Genomic Unit (PGU) and UCL Genomics, University College London (UCL), United Kingdom. This document describes the manual steps to perform the protocol, but for further information about the automation of the protocol, please, contact Pathogen Genomics Unit (PGU) - UCL. Manager: Rachel Williams https://www.ucl.ac.uk/infection-immunity/pathogen-genomics-unit This protocol is based on the amplicon tiling strategy described previously by Quick J et al 2017. However, we have applied this strategy to recover long reads (2kb), then some adjustments were performed in the protocol. If you have doubts, we are happy to help you to test or implement the protocol.
A protocol for rapid western-blot: shorten the time to 1-3 hours v1
Authors: Zhao Shenao Biotechnology Company Zhichao , jiang wang
Published: May 2020

Western blot (WB) is a classical and powerful tool to detect the level of interested protein from among a mixture of proteins. Since its invention in 1979, WB is a time-consuming technique that usually needs 1 to 2 days to obtain the result. Here, being aware of the time spent by the researcher, we share a rapid protocol that shortens the time up to 1 hour for detecting the internal loading control protein actin (42kDa) when using the HRP-conjugated anti-actin antibody. Additionally, the time for detecting other (21kDa-100kDa) could be shortened to 3 hours when using the second HRP-conjugated antibody. In conclusion, this rapid protocol dramatically improved the efficiency of WB without increasing the experiment cost or losing its high data quality.
10% Tween 20 v1
Authors: Institute for Brain Science Allen
Published: May 2020

This protocol is used to prepare 10% Tween 20. Tween 20, when added to aqueous solutions acts as a surfactant, decreasing the surface tension of the solution. It also punches holes in membranes, allowing easier penetration of macromolecules into cells and organelles, and reduces non-specific binding of antisera in immunohistochemistry. Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Interactivity of Simultaneous Interpreters as Actors of a Cognitive Event
Authors: Chistova Elena V.
Published: May 2020

The paper refers to studying interactivity models of simultaneous interpreters in the field. It describes the process of simultaneous interpretation as a cognitive event considering two participants in the context of cognitive dissonance and problem solving. The key concept of identifying the models is empathy to a booth mate which has never been studied in the context of Cognitive Translation Theory before. The results of Cognitive Event Analysis show four main interactivity models: empathic productive, empathic unproductive, empathic destructive and no empathic. The examples of empathic productive interactivity model demonstrate both material, verbal and cognitive empathy. The trajectories of giving a hint show three obligatory cycles of creating productive empathy: identifying cognitive dissonance, illustrating emphatic behavior and inviting to interactivity. The most precious hint happens when deep cognitive empathy between the partners can encourage them by embodied help without any verbal or material hints. It includes two additional cycles: mutual cognitive dissonance and individual attempt to solve the problem. This model is observed mainly in pairs of highly qualified interpreters with enough experience of coupling. Novice simultaneous interpreters are not able to show empathy, so tutors should train the skill of overcoming this gap. Empathic unproductive or destructive interactivity models are observed in pairs of highly qualified interpreters with no experience of coupling because of incongruent mental processes of subject and object
LEGAL MODELS FOR MARRIAGE AND MARRIAGE-LIKE UNIONS IN THE LAW OF EU STATES
Authors: Radchenko L.
Published: May 2020

The article considers the comparative and legal grounds for the definition of the category "marriage" in family law of Ukraine, interpretation of the concept of "marriage-like unions" in legislation, judicial decisions and legal doctrine of EU states. The author defines the characteristics and peculiarities of these concepts, outlines the approaches to the regulation of family relations in this field and formulates conclusions and proposals aimed at the harmonization of national legislation with EU law.

In the article, both general scientific and special legal methods of research have been used. In particular, comparative-legal, historical, formallogical and other methods have been applied. The article concludes that the example of some European states proves the existence of the advanced legislative approaches to the legal understanding of family unions.

In Ukraine the marriage is exclusively a family union of a female and a male registered with the state registration authority for civil status acts. In the laws of EU states different and much broader criteria for treating such unions can be applied. The presence of various legal forms of unions indicates that foreign law recognizes a family union between persons irrespective of their genders and recognizes such family union as a family. It is said that a characteristic feature for a family is a stable relationship. At the same time, harmonization of the Ukrainian legislation with the EU legislation in the field of marriage and family relations may take place on specific issues (for example, a registered partnership), but it is not advisable to adopt a foreign legal model, since in Ukraine there are distinctive factors for the formation of family relationships, such as traditions, religion, culture.
Creative metaphors in video lessons: the relationship between the novel and the conventional / Metáforas criativas em videoaulas: a relação entre o novo e o convencional
Authors: Schabarum John Richart , Chishman Rove Luiza de Oliveira
Published: May 2020

Abstract: The metaphors used in non-poetic contexts are mostly conventional. This study, which is part of a broader research, analyzes the emergence of – and the relationship between – conventional and novel/creative metaphors in Biology video lessons. The theoretical basis is composed of the Theory of Conceptual Metaphor (LAKOFF; JOHNSON, 2002; LAKOFF; TURNER, 1989) and the Perspective of Metaphor in Discourse (SEMINO, 2008). Thus, the general objective of this paper is to verify the emergence of conceptual and linguistic metaphors produced by teachers in Biology video lessons, as well as to analyze their degree of novelty and/or conventionality. The data analyzed came from YouTube video subtitles, and the methodology used to identify metaphors in speech was the MIP (PRAGGLEJAZ GROUP, 2007). The results indicate that creative metaphors are present in the discourse of these teachers and play a relevant role in the presentation of scientific content.Keywords: metaphor; creativity; conventionality; video lessons; textual patterns.Resumo: As metáforas empregadas em contextos não poéticos apresentam-se majoritariamente de forma convencional. O presente estudo, que faz parte de uma pesquisa mais abrangente, trata da análise da emergência de – e da relação entre – metáforas convencionais e novas/criativas em videoaulas de Biologia. O aporte teórico é composto pela Teoria da Metáfora Conceptual (LAKOFF; JOHNSON, 2002; LAKOFF; TURNER, 1989) e pela Perspectiva da Metáfora no Discurso (SEMINO, 2008). Desse modo, o objetivo geral deste artigo é verificar a emergência de metáforas conceptuais e linguísticas produzidas por professores em videoaulas de Biologia, assim como analisar seu grau de novidade e/ou convencionalidade. Os dados analisados são provenientes de legendas de videoaulas do YouTube, e a metodologia empregada na identificação de metáforas no discurso foi o MIP (PRAGGLEJAZ GROUP, 2007). Os resultados indicam que metáforas criativas estão presentes no discurso desses professores e desempenham um papel relevante na apresentação de conteúdos científicos.Palavras-chave: metáfora; criatividade; convencionalidade; videoaulas; padrões textuais.
Women’s representations in advertisements: conceptual explorations / As representações das mulheres em propagandas: explorações conceptuais
Authors: Souza Amanda Maria Bicudo de
Published: May 2020

Abstract: Unlike traditional visions show, metaphors are present not only in literary language, but also in everyday, scientific and philosophical languages. It is through the metaphors that we conceptualize the world and understand the most abstract concepts that we have. Based on this premise, this study investigates the conceptual metaphors found in advertisements of women’s magazines, based on the theories: the theory of conceptual metaphor proposed by Lakoff and Johnson (2002), considerations of Kovecses (2002) about this theory and particular character of metaphors in advertisements, as proposed by Velasco-Sacristán (2010) and Ungerer (2003). The purposes of this research are to examine how the metaphors conceptualize the product advertised and how they reveal the social representation of women. The research was done through analysis of four advertisements taken from the magazines: Nova (Dec. 2008), Marie Claire (Oct. 2008), Cláudia (July 2009) and one advertisement taken from a website, published in 2020. The results show that conceptual metaphors are tools used by propagandists in the creation of advertisements based on stereotypes, representations and ideologies of particular group to which the advertisement is intended. Such resources tend to persuade the reader to purchase the product released.Keywords: metaphor; conceptual metaphor; advertisements.Resumo: Ao contrário do que mostram visões tradicionais, a metáfora não está presente somente na linguagem literária, mas também nas linguagens: cotidiana, científica e filosófica. É por meio das metáforas que nós conceptualizamos o mundo e compreendemos a maioria dos conceitos abstratos que temos. Com base nesse postulado, o presente estudo investiga as metáforas conceptuais presentes em propagandas de revistas femininas, tendo como pressupostos teóricos: a teoria da Metáfora Conceptual, proposta por Lakoff e Johnson (2002), as considerações de Kovecses (2002) acerca dessa teoria e o caráter particular das metáforas em propagandas, como proposto por Velasco-Sacristán (2010) e Ungerer (2003). Os objetivos dessa investigação são verificar o modo como as metáforas conceptualizam o produto divulgado e em que medida elas revelam a representação social da mulher. A pesquisa foi realizada através da análise de duas propagandas, retiradas das revistas: Nova (dez. 2008) e Marie Claire (out. 2008). Os resultados mostram que as metáforas conceptuais são ferramentas utilizadas pelos propagandistas na elaboração das propagandas, com base em estereótipos, representações e ideologias de determinado público-alvo ao qual a propaganda é destinada. Tais recursos tendem a persuadir o leitor a adquirir o produto divulgado.Palavras-chave: metáfora; metáfora conceptual; propagandas.
Multimodal Metaphors of Interculturereality / Metaforas multimodais da interculturealidade
Authors: Ladilova Anna
Published: May 2020

Abstract: The present paper looks at the interactive construction of multimodal metaphors of interculturereality – a term coined by the author from interculturality and intercorporeality, assuming that intercultural interaction is always an embodied phenomenon, shared among its participants. For this, two videotaped sequences of a group conversation are analyzed drawing upon interaction analysis (Couper-Kuhlen; Selting, 2018). The data was transcribed following the GAT2 (Selting et al., 2011) guidelines, including gesture form annotation, which relied on the system described by Bressem (2013). Gesture function was interpreted drawing on the interactional context and on the system proposed by Kendon (2004) and Bressem and Müller (2013). The results question the validity of the classical conduit metaphor of communication (Reddy, 1979) in the intercultural context and instead propose an embodied approach to the conceptualization of the understanding process among the participants. The analysis also shows that even though the metaphors are multimodal, the metaphoric content is not always evenly distributed among the different modalities (speech, gesture). Apart from that, the metaphorical content is constructed sequentially, referring to preceding metaphors used by the same or different interlocutors and associated with metaphorical blends.Keywords: metaphors; multimodality; interculturality; intercorporeality; migration.Resumo: O presente artigo analisa a construção interativa de metáforas multimodais da interculturealidade – um termo proveniente da interculturalidade e intercorporealidade, assumindo que a interação intercultural é sempre um fenômeno incorporado, compartilhado entre os seus participantes. Para tal, duas sequências gravadas em vídeo de uma conversa em grupo serão analisadas com base na análise da interação (COUPER-KUHLEN; SELTING, 2018). Os dados foram transcritos seguindo as orientações do sistema GAT2 (SELTING et al., 2011), incluindo a anotação da forma gestual, que se baseou no sistema descrito por Bressem (2013). A função dos gestos foi interpretada com base no contexto interacional e no sistema proposto por Kendon (2004) e Bressem e Müller (2013). Os resultados questionam a validade da metáfora clássica do conduto de comunicação (REDDY, 2012) no contexto intercultural e, além disso, propõem uma abordagem corporificada da conceituação do processo de entendimento entre os participantes. A análise também mostra que, embora as metáforas sejam multimodais, o conteúdo metafórico não é sempre uniformemente distribuído entre as diferentes modalidades (fala, gesto). Além disso, o conteúdo metafórico é construído sequencialmente, referindo-se a metáforas anteriores utilizadas pelos mesmos ou diferentes interlocutores e recorrendo a mesclagens metafóricas.Palavras-chave: metáforas; multimodalidade; interculturalidade; intercorporealidade; migração.
Breidel en stimulans
Authors: Harbers Frank , Van Oort Thunnis
Published: May 2020

Editorial
‘Ils ont bombardé Reims et nous avons vu cela!’
Authors: Harbers Frank
Published: May 2020

De Eerste Wereldoorlog beteugelde niet alleen de vrije informatiegaring van de Britse, Nederlandse en Franse pers,maar gaf ook een impuls gaf aan de ontwikkeling van de professionele praktijk van de journalistiek. In het oorlogsgebied en in de buurt van het strijdtoneel raakten de journalisten doordrongen van de verantwoordelijkheid die op hun schouders rustte. Ondanks de ingrijpendemaatregelen van de autoriteiten omhun verslaggeving te beteugelen en te controleren, poogden deze verslaggevers om een betrouwbaar beeld van de oorlogssituatie te schetsen. Een analyse van oorlogsreportages van Britse, Nederlandse en Franse journalisten, respectievelijk William Beach Thomas en George Ward Price, Jean Louis Pisuisse en Johan Lüger, en Albert Londres, laat zien hoe de oorlog deze verslaggevers dwong om op hun eigen ogen en oren te vertrouwen en op basis van hun observaties een impressie van de werkelijkheid te geven. Ze zagen hun status als ooggetuige als teken van autoriteit en betrouwbaarheid. De nadruk op de ‘mediating subjectivity’ van de verslaggever betekende echter niet dat er al sprake was van de opkomst van het objectiviteitsregime.
Memorias del Tercer Simposio en Fitopatología, Control Biológico e Interacciones Planta-Patógeno
Authors: León-Reyes Antonio , Barriga Noelia
Published: May 2020

Memorias del Tercer Simposio en Fitopatología, Control Biológico e Interacciones Planta-Patógeno
Memorias del 4to Simposio en Fitopatología, Control Biológico e Interacciones Planta- Patógeno
Authors: León-Reyes Antonio , Barriga Noelia
Published: May 2020

Memorias del 4to Simposio en Fitopatología, Control Biológico e Interacciones Planta- Patógeno
Effects of families’ socioeconomic and education level on auditory skills of cochlear implant users: an Iranian population study
Authors: Moosapour Mohammadsaleh , Ajalloueyan Mohammad , Zamiri Abdollahi Farzaneh , Delphi Maryam , Berri Dizaji Ali
Published: May 2020

Background and Aim: Hearing loss can have disabling effects on all aspects of children’s life and demographic factors of families can have significant effects on children’s auditory deve­lopment. The main aim of the study was deter­mining the effects of socioeconomic and educa­tion level on auditory behaviors of hearing-impaired children.
Methods: The study was cross sectional descrip­tive-analytic study and was conducted on 207 parents of children under age of four years with native Persian speaking parents with literacy skill. Their hearing impairment was identified before the first month of age. Early occurrence of hearing loss was considered to exclude any effe­cts of early exposure to normal auditory stimuli on the outcome measurements. The children had at least 3-month experience with the cochlear implant (CI) after best fitting and adaptation to their device at the time of the study. Samples were selected by convenience sampling method from available subjects. For determining socioeconomic level, Ghodratnama socioeco­nomic status (SES) questionnaire was used. Infa­nts and Toddlers Meaningful Auditory Integ­ration Scale (IT-MAIS) was selected for the auditory beha­viors study.
Results: Socioeconomic and educational level of the family showed no significant effects on audi­tory behaviors. The age of receiving auditory ass­istive device had weak but statistically signifi­cant effect on the outcome.
Conclusion: It seems that socioeconomic status and educational level of the family did not con­tribute to the auditory behaviors of children with cochlear implant. The generalizations of these results need further studies.
Keywords: Cochlear implant; pediatric; auditory behaviors; socioeconomic status
The Association of Potato Consumption and Psychological Disorders in Women: A Cross-Sectional Study
Authors: Darooghegi Mofrad Manije , Siassi Fereydoun , Guilani Bijan , Azadbakht Leila
Published: May 2020

Background: Previous studies reported controversial findings regarding the association of potato consumption with the risk of chronic diseases. The purpose of this study was to determine the relationship of potato consumption with depression, anxiety, and stress in Tehrani women.
Methods: In this cross-sectional study, 488 women aged 20-50 years old who referred to health centers affiliated with Tehran University of Medical Sciences were selected by multistage cluster sampling. Their usual dietary intake in the past year was evaluated using a semi-quantitative food frequency questionnaire containing 168 items with confirmed validity and reliability. Psychological disorders were assessed using a validated Depression, Anxiety, and Stress Scales (DASS) questionnaires with 21-items. In the logistic regression analysis, the results were adjusted to the confounding factors.
Results: After adjustment of the confounding variables, consuming potatoes had no significant association with depression (P = 0.12), anxiety (P = 0.19), and stress (P = 0.63). Furthermore, consuming boiled potatoes had no significant association with depression (P = 0.59), anxiety (P = 0.19), and stress (P = 0.37).  Intake of fried potatoes had no relationship with depression (P = 0.16), anxiety (P = 0.27), and stress (P = 0.97).
Conclusion: Potato consumption has no significant relationship with depression anxiety and stress in Tehrani women.