bluuebunny/crossref_metadata_2025 huggingface dataset:
Nos museus, em torno dos museus
Authors: Margarito Mariagrazia , Orlandi Eni Puccinelli
Published: December 2020

Quando um projeto, que reuniu universidades dos dois hemisférios do nosso planeta, leva à publicação dos seus resultados, levando também em consideração os tempos tão difíceis em que vivemos, é momento para felicitações e satisfação por um trabalho em que múltiplas competências analisaram um "objeto social" dentro do quadro teórico da análise de discurso. O objeto social em questão é o texto expográfico, este texto escrito, colocado ao lado das obras, de objetos expostos, em diferentes lugares das salas de um museu, de uma exposição, seja qual for a tipologia desses lugares urbanos: museu de belas artes, de ciências, da vida quotidiana… Texto que acompanha a visita pública, mas não só.
Tensions et passions des textes expographiques
Authors: Margarito Mariagrazia
Published: December 2020

Dans le cadre des disciplines linguistiques, de l’analyse du discours d’école française en privilégiant les données lexicales et les items phrastiques, nous présentons quelques points sur lesquels nous nous sommes penchée dans nos études sur les textes expographiques, ces écrits qui s’affichent dans les musées, les expositions, aux côtés des œuvres. Nous avons relevé la complexité de ces textes, redevable non seulement aux typologies de musées et d’expositions où ils sont affichés, mais surtout à leur nature hybride, à la tension permanente entre fonction didactique de partage des savoirs et souci de ne pas décevoir les attentes des visiteurs pour qui avoir du plaisir lors d’une visite est perçu comme ressentir des émotions. Énoncée ou suggérée, dans la matérialité de la langue, l’empathie avec le public peut se manifester sous des formes variées, à différents endroits du texte lui-même.
Discurso artístico no metrô
Authors: Nunes José Horta
Published: December 2020

Este trabalho apresenta uma análise do discurso de divulgação artística em estações da “linha verde” do metrô paulistano, na região da Avenida Paulista. Com base em textos institucionais, observações no local, registros fotográficos e simulações da perspectiva dos transeuntes, explicita-se o discurso sobre a arte no metrô. São analisados painéis de divulgação artística dispostas na plataforma, bem como os sujeitos em trânsito. A perspectiva teórica é a da Análise de Discurso, especialmente os estudos discursivos sobre a cidade (ORLANDI [Org.], 2001a; ORLANDI, 2004, 2017) e estudos de textos expográficos (RIGAT, 2015). As estações são concebidas como espaços de interpretação, sítios de significação (ORLANDI, 2004). Observamos também as relações entre os sujeitos e os espaços citadinos, na tensão entre o real da cidade e sua organização institucional. As análises mostram diferentes sentidos ligados aos espaços de transição das estações, conforme os posicionamentos dos sujeitos diante das obras de arte, no interior do trem e na plataforma da estação.
Museu de Favela e biografias urbanas
Authors: Costa Greciely Cristina da
Published: December 2020

A partir do domínio Saber Urbano e Linguagem e da compreensão de museu como instituição que atua nos processos de individuação do sujeito, se configurando também como prática de significação (ORLANDI, 2013a), tecemos algumas considerações sobre o conceito de museu de território. Num gesto de apropriação teórica, refletimos discursivamente sobre este conceito para observar as especificidades do Museu de Favela como uma das formas do discurso urbano. Como resultado, desenvolvemos a noção de biografia urbana e analisamos seu funcionamento no processo discursivo que chamamos biografização.
formação da elite militar brasileira
Authors: Marcusso Marcus
Published: December 2020

A Escola de Estado-Maior (EEM) foi criada em 1905, e tinha como principal objetivo fornecer aos oficiais do Exército Brasileiro uma instrução militar superior que os habilitasse para exercer funções no Estado-Maior do Exército. O presente artigo tem como principal objetivo compreender as mudanças ocorridas na educação militar brasileira, de forma geral, e na Escola de Estado-Maior do Exército, de forma específica, durante a breve, mas intensa, gestão do General Góes Monteiro no Ministro da Guerra entre 1934 e 1935. Para tanto, foram consultados documentos oficiais (regulamentos de ensino, relatórios do ministério da guerra e da chefia do Estado-Maior do Exército), além de memórias e relatos memorialísticos de ex-alunos da Escola de Estado-Maior. O estudo sobre a Escola de Estado-Maior do Exército proporciona uma compreensão aprofundada sobre a formação do oficial-aluno do Exército Brasileiro, e as relações estabelecidas entre militares e civis nas primeiras décadas do século XX.
Das trincheiras para a terceira guerra
Authors: Oliveira Edilan Martins de
Published: December 2020

Este trabalho se destina a efetuar uma análise sobre a constituição da Associação dos Ex-Combatentes do Brasil em Belo Horizonte após o término da Segunda Guerra Mundial (1939-1945). Pautando-se principalmente nas atas redigidas por ocasião das reuniões ocorridas na agremiação, se buscará efetuar um panorama acerca das concepções, posições e dilemas enfrentados pelos veteranos no imediato pós-guerra. Nesse período, a associação assumiu um caráter assistencial, e, para além da realização de comemorações e da efetivação de ações que visavam à difusão da memória da coletividade em questão, foi marcada preponderantemente por disputas políticas acirradas. A difícil reintegração social, após a desmobilização da Força Expedicionária Brasileira, engendrou formas de resistência e negociação singulares com os poderes públicos, assim como moldou as ações dos outrora combatentes no interior da referida agremiação. Torna-se fundamental expor as narrativas e os pensamentos que foram concebidos por esse grupo social no imediato reestabelecimento da democracia, após o fim do Estado Novo (1937-1945).
Militares e militarismos
Authors: Robba Olívia da Rocha , Santos Wagner Luiz Buenos dos
Published: December 2020

É com grande satisfação e alegria que apresentamos aos leitores da Revista Temáticas o dossiê "Militares e militarismos: perspectivas interdisciplinares sobre o universo militar." Composto por 11 artigos que analisam as Forças Armadas, suas atividades, pensamento e capilaridade em relação a sociedade, este dossiê é uma tentativa de lançar luz a temas e problemas que envolvem o universo militar. Com a intenção de contribuir para melhor entendimento sobre esse universo, os autores aqui reunidos trouxeram discussões que abordam e ampliam a visão sobre a caserna, seja com antigos temas sobre novos olhares seja com novos temas que passaram a chamar atenção de historiadores, antropólogos, cientistas sociais e pesquisadores que se interessam sobre questões que envolvem os militares.
PERCEPÇÃO DE SUCESSO NA CARREIRA: SEMELHANÇAS E DIFERENÇAS ENTRE PROFISSIONAIS DO SETOR PÚBLICO
Authors: De Lima Tereza Cristina Batista , Paiva Luis Eduardo Brandão , Neto Ernesto de Oliveira Aderaldo , De Aquino João Paulo Cavalcante
Published: December 2020

A presente pesquisa tem como objetivo avaliar a percepção de sucesso na carreira dos profissionais de uma empresa de administração portuária, ligada ao setor público, procurando diferenciar a percepção de sucesso na carreira entre funcionários efetivos e terceirizados. Partiu-se das hipóteses de que os indivíduos percebem suas carreiras de diversas maneiras, considerando as percepções objetivas e subjetivas de sucesso profissional, tomando por base a percepção a partir do tipo de vínculo empregatício.
SISTEMA DE REMUNERAÇÃO: ESTUDO EM UMA EMPRESA DESENVOLVEDORA DE SOFTWARE
Authors: Marcelino Aline Faxina
Published: December 2020

Este estudo tem como propósito investigar o sistema de remuneração e sua contribuição no alcance dos resultados organizacionais na percepção dos funcionários. Para tal foi desenvolvido uma pesquisa exploratória em uma empresa de software localizada no noroeste do estado do Paraná que desenvolve programas de gestão integrada para o gerenciamento de empresas. Para atender aos objetivos da pesquisa os dados foram coletados por meio de entrevistas semi-estruturadas com 06 funcionários, sendo 05 deles programadores e 01 gerente de Recursos Humanos. Dessa maneira foi possível identificar que, apesar dos programadores não terem o pleno conhecimento acerca do sistema de remuneração utilizado pela empresa, há uma percepção positiva deste. Assim eles acreditam que, o sistema de remuneração os incentiva a buscar melhores resultados dentro da empresa, entretanto, foi identificado o desejo dos programadores por mudanças, tanto na política salarial quanto nos benefícios que esta concede. Assim, por meio do estudo, foi possível evidenciar que, é necessário que a empresa conscientize seus funcionários sobre o seu sistema remuneratório, seu significado e do que é constituído para que assim eventuais mudanças sejam percebidas de um modo favorável à organização. Além disso, os programadores manifestaram interesse em benefícios relacionados à saúde e à produtividade.
TRANSIÇÃO NA CARREIRA E A QUANTOFRENIA: UM ESTUDO DE CASO COM COORDENADORES DE MESTRADO E DOUTORADO DE UMA IES PRIVADA
Authors: Oltramari Andrea Poleto
Published: December 2020

O artigo tem como objetivo compreender como gestores de programas de mestrado e doutorado de uma Instituição de Ensino Privada percebem a construção de suas carreiras, vivenciam a transição para assumirem o cargo de gestores e como lidam com a quantofrenia gerencialista. Para tanto, participaram da pesquisa oito professores doutores coordenadores Stricto Sensu de cursos de pós-graduação de uma IES privada. Os principais resultados evidenciaram que o trabalho se apresentou precarizado; na transição na carreira a maioria dos coordenadores não tem tempo de preparação para assumirem os novos cargos; a quantofrenia é intensa e a adesão ao projeto organizacional e aos índices da Capes redundam em difícil conciliação da vida pessoal e profissional.
UMA ABORDAGEM COMPARATIVA DOS PLANOS DE CARREIRAS E CARGOS DO MAGISTÉRIO SUPERIOR FEDERAL
Authors: Gomes Carlos Francisco Simões , ALVES MARCIA RODRIGUES
Published: December 2020

A educação pública superior busca a eficiência, especialmente no âmbito da remuneraçãoe motivação docente. Para comparar o antes e o depois do plano de carreiras das instituiçõesfederais de ensino, este texto propõe, mediante revisão bibliográfica e documental, e análisecomparativa, verificar os efeitos desta alteração para a gestão de pessoas dessas instituições.Assim, dentre os resultados, observou-se o aumento na participação da titulação de 55,35%da remuneração, e aumento de 1.339% em seus valores, quando comparados aos valores doplano anterior. Conclui-se que o maior benefício do novo plano foi a valorização da titulaçãoacadêmica na formação docente.
PARA ALÉM DO FIM DO ARCO-ÍRIS: MOTIVAÇÕES E CONSEQUÊNCIAS DA ADESÃO AO PLANO DE DEMISSÃO VOLUNTÁRIA
Authors: Silva Ligia Oliveira , Vieira Jeanine Ângela , Baidek Adriana Cássia Ribeiro
Published: December 2020

O objetivo da presente pesquisa foi investigar o contexto que envolve a adesão aoprograma de desligamento voluntário e quais seus impactos na carreira profissional. Osparticipantes eram ex-funcionários de diferentes instituições, localizadas em Brasília/DF. Paraa análise dos dados foi utilizado o método de Análise de Conteúdo. Os principais resultadosmostram que o motivo mais relevante para aderir ao plano foi o esgotamento psíquico e a faltade incentivo profissional no trabalho. Conclui-se que o Plano de Demissão Voluntária é vistocomo uma alternativa para investir em projetos que não seriam possíveis ou conciliáveis coma carreira anterior.
Editorial
Authors: Trevisan Leonardo , Dutra Joel Souza , Velozo Elza
Published: December 2020

As pressões do cenário econômico, ou as tensões do ambiente organizacional, em suas múltiplas origens, definem diferentes formatos para o desenvolvimento das carreiras. Essa constatação, no entanto, omite inúmeras atitudes de mobilidade na gerência das expectativas profissionais, tanto do indivíduo como das organizações. Nosso conhecimento dos mecanismos que incentivam os reordenamentos da vida profissional é reconhecidamente incompleto. Compreender melhor a “lógica de mobilidade”, que hoje marca tanto carreiras como gestão de pessoas, é o eixo temático do primeiro número do Volume 6 da Revista de Carreiras e Pessoas
The grouping of industrial enterprises by capacity utilization rate and level of debts to banks (IV Quarter of 2019)
Authors: Aukutsionek S. , Dyomina N. , Bashirova I. , Serzhantova T. , Belyakov S.
Published: December 2020

Russian industrial enterprises, examined by the Russian Economic Barometer are grouped according to two criteria: the level of capacity utilization rate, as well as the level of debts to banks. This grouping allows you to see how key characteristics of enterprises differ depending on how their production capacities are loaded and what level of debts to banks they have. Data are presented for the fourth quarter of 2019.
Crossing the Border:
Authors: smith alan e.d.
Published: December 2020

Synopsis

The response of the Papua New Guinea government to the refugee influx of 1984 was conditioned by the Australian administration’s approach to border management prior to Papua New Guinea independence and by the Papua New Guinea - Indonesia border agreement. This approach is one of containment.
The unprecedented scale of the refugee influx in 1984 caused a policy crisis for Papua New Guinea. Efforts to encourage refugees to return home met resistance from the refugees and caused public controversy. In 1985 when West Papuan refugees arrived in Australia for the first time, the Australian government demonstrated it's continuing commitment to the policy of containment which was reflected in its refusal to support Papua New Guinea's attempts to internationalise the issue.
The refugee influx afforded Papua New Guinea a legitimate opportunity to press for the cause of the problem to be examined. But it's attempts to do so were abandoned for want of international, crucially Australian, support. A massive influx of refugees should automatically set in motion an international process to examine root causes of the influx in order to bring about the necessary conditions for voluntary repatriation.
The achievement of the changes in Irian Jaya necessary to resolve the conflict between West Papuan nationalists and the Indonesian state may depend on the creation of new international measures to address the whole class of frustrated claims to the right of self-determination.
What is needed is an authoritative international process through which self-determination claims can be assessed and a range of forms through which the aspirations they represent can be satisfied.
Sports Medicine Roundtable
Authors: Collins Larry , Dowell Charles , Lefkowitz Melissa , Downie Brian , Cloutier Dagan
Published: December 2020

The purpose of the JOPA Roundtable discussion was to give orthopaedic physician assistants an insight on how each participating editorial board members treat certain clinical scenarios in their current practice. For this Sports Medicine Roundtable, editorial board members with expertise in sports medicine will discuss the management of osteochondral dissecans lesions in young athletes. Participants will share their knowledge and experience on the topic. The roundtable discussion will start with 2 brief case presentations.
Web-scale Discovery Service Adoption in Canadian Academic Libraries
Authors: Wong Sandra
Published: December 2020

This study presents an overview of web-scale discovery service adoption in Canadian academic libraries. Web-scale discovery services (WSDS) have been widely embraced in Canadian academic libraries. EBSCO Discovery Service (EDS) is the most adopted system for colleges and institutes while Primo Central from Ex Libris dominates the university market for web-scale discovery services. Bundling web-scale discovery services with library services platform (LSP) implementations are increasing in Canada. This study shows that Canadian academic libraries that have migrated from a traditional integrated library system (ILS) to an LSP have also opted for their LSP vendor’s corresponding web-scale discovery service. As more Canadian academic libraries implement an LSP bundled with a web-scale discovery service, there may come a time when web-scale discovery services are no longer separated from discussions on LSPs. The choices available for LSP and web-scale discovery services are shrinking due to vendor mergers and acquisitions in this area.
The Fabulousness and the Fury: Preparing for a Drag Queen Storytime
Authors: Davey Douglas
Published: December 2020

After announcing the launch of a Drag Queen Storytime, the Halton Hills Public Library (HHPL) received positive feedback from the community, but also faced criticism from a small but vocal group of citizens. Complaints reached as high as the mayoral office, challenging the library’s position as a trusted community institution, and testing the rigour of its training, communications, policies, and procedures. Despite the pushback, the library ran a highly successful program and afterward redoubled its commitment to fighting homophobia and promoting equality. This article details how the HHPL came to offer the program, what the response was, as well as suggestions for measuring success and processing complaints.
Investigar, escribir y publicar. ¿Qué tan importante es para la universidad? Caso de estudio hasta el 2018
Authors: Arias-Zapata Daniela Andrea
Published: December 2020

Este artículo es el resultado del trabajo realizado durante un año como asistente editorial de una revista científica colombiana. Plantea una crítica al modelo de medición generado y utilizado hasta el año 2018 por las diferentes editoriales mediante los indicadores utilizados por Colciencias –hoy Minciencias–, a fin de clasificar y categorizar las investigaciones, donde el noble arte de investigar se ha tergiversado al pasar de ser una actividad de avance y progreso por la que se mejora la calidad del conocimiento, a convertirse en un banco de conocimiento por cantidad y competitividad. En el texto se presenta un esbozo crítico sobre el papel de la universidad y la ciencia en relación con la investigación y el impacto que la implementación de las mediciones trae para los articulistas e investigadores. También se dan a conocer las limitaciones y las falencias en el mecanismo que utilizan los indicadores de medición estandarizados para la clasificación de «calidad» investigativa. Y, finalmente, se identifican los componentes de un artículo científico para que este sea publicable y se señalan los pasos para escoger la revista científica que garantice la publicación del artículo, así como las mediciones a las que están sujetas tanto las revistas como los artículos científicos.
Pexophagy Protects Plants from Reactive Oxygen Species-induced Damage under High-intensity Light
Authors: oikawa Kazusato , Goto-Yamada Shino , Hayashi Yasuko , Takahashi Daisuke , Kimori Yoshitaka , Shibata Michitaro , Yoshimoto Kohki , Takemiya Atsushi , Kondo Maki , Kato Akira , Shimoda Keisuke , Ueda Haruko , Uemura Matsuo , Numata Keiji , Ohsumi Yoshinori , Hara-Nishimura Ikuko , Mano Shoji , Yamada Kenji , Nishimura Mikio
Published: December 2020

Light is essential for photosynthesis, but it has the potential to elevate intracellular levels of reactive oxygen species (ROS) during photosynthesis. Photorespiration is a metabolic pathway in photosynthesis to metabolise oxidised by products from chloroplasts, and it generates a high level of ROS in peroxisomes. Since high levels of ROS are toxic, plants must manage damage from ROS. However, the cellular mechanism to elude leaf damage from ROS in peroxisomes is not fully explored. Here we show that autophagy plays a pivotal role in the selective removal of ROS-generating peroxisomes, which protects plants from oxidative damage during photosynthesis. We found that a series of peup mutants, which is a defect in autophagy degradation of peroxisomes, showed light-intensity-dependent leaf damage and excess aggregation of ROS-accumulating peroxisomes. The peroxisome aggregates were specifically engulfed by pre-autophagosomal structures and vacuolar membranes, but they were not degraded in the mutants. ATG18a-GFP and GFP-2 × FYVE, which both bind to phosphatidylinositol 3-phosphate, preferentially targeted the peroxisomal membranes and pre-autophagosomal structures near peroxisomes in ROS-accumulated cells under high-intensity light conditions. Our findings provide new information to better understand the plant stress response caused by light irradiation.
Molecular profile of KRAS G12C-mutant colorectal and non-small-cell lung cancer
Authors: Araujo Luiz H. , Souza Bianca , Leite Laura , Parma Sabrina , Lopes Natália , Malta Frederico , Freire Maíra
Published: December 2020

Background: KRAS is the most frequently mutated oncogene in cancer, however efforts to develop targeted therapies have been largely unsuccessful. Recently, two small-molecule inhibitors, AMG 510 and MRTX849, have shown promising activity in KRAS G12C-mutant solid tumors. The current study aims to assess the molecular profile of KRAS G12C in colorectal (CRC) and non-small-cell lung cancer (NSCLC) tested in a clinical certified laboratory.Methods: CRC and NSCLC samples submitted for KRAS testing between 2017 and 2019 were reviewed. CRC samples were tested for KRAS and NRAS by pyrosequencing, while NSCLC samples were submitted to next generation sequencing of KRAS, NRAS, EGFR, and BRAF. Results: The dataset comprised 4,897 CRC and 4,686 NSCLC samples. Among CRC samples, KRAS was mutated in 2,354 (48.1%). Most frequent codon 12 mutations were G12D in 731 samples (15.2%) and G12V in 462 (9.6%), followed by G12C in 167 (3.4%). KRAS mutations were more frequent in females than males (p=0.003), however this difference was exclusive of non-G12C mutants (p<0.001). KRAS mutation frequency was lower in the South and North regions (p=0.003), but again KRAS G12C did not differ significantly (p=0.80). In NSCLC, KRAS mutations were found in 1,004 samples (21.4%). As opposed to CRC samples, G12C was the most common mutation in KRAS, in 346 cases (7.4%). The frequency of KRAS G12C was higher in the South and Southeast regions (p=0.012), and lower in patients younger than 50 years (p<0.001). KRAS G12C mutations were largely mutually exclusive with other driver mutations; only 11 NSCLC (3.2%) and 3 CRC (1.8%) cases had relevant co-mutations. Conclusions: KRAS G12C presents in frequencies higher than several other driver mutations, represent a large volume of patients in absolute numbers. KRAS testing should be considered in all CRC and NSCLC patients, independently of clinical or demographic characteristics.
Operationalization of “urethral calibration protocol” to develop skills among caregivers and nursing personnel dealing with children undergoing urethroplasty
Authors: Lohchab Sweety , Kaur Rupinder , Kaur Sukhwinder , Mahajan J.K.
Published: December 2020

Hypospadias is considered as second most common birth defect in children with 1 in every 250 male births worldwide and 1 in every 150 boys in India. Urethroplasty is only mode of hypospadias repair which is associated with wide range of complications like urethrocutaneous fistula, meatal stenosis, narrow urethra. Rising incidence of these complications demands judicious postoperative care and urethral calibration. Objective: To develop and operationalize protocol on urethral calibration for children undergone urethroplasty among caregivers and nursing personnel. Method: An operational study was conducted on caregivers and nursing personnel at pediatric surgery OPD. Total of 40 study subjects i.e. 32 caregivers and 8 nursing personnel included by total enumeration sampling. Study was conducted in three phases. In 1st phase, current practices of urethral calibration were assessed.10 observations were taken to identify lacunae regarding procedure. Protocol on urethral calibration was developed by extensive literature review, expert opinions using Delphi technique. In 2nd phase, training sessions were conducted using lecture cum discussion, demonstration and return demonstrations, booklets and posters. In 3rd phase, assessment of effectiveness of urethral calibration was done by evaluating occurrence of complications related to urethroplasty. Results: Significant (p < 0.001) increase in mean performance score of urethral calibration of caregivers and nursing personnel was achieved up to 22.75 ±1.10 and 23.50 ± 0.92 after four and three subsequent return demonstrations respectively. Conclusion: Protocol on urethral calibration is effective in developing skills among caregivers and nursing personnel related to procedure and improving surgical outcome of urethroplasty among children.
Enhancing the Catalysis of Oxygen Reduction Reaction via Tuning Interfacial Hydrogen Bonds
Authors: Wang Tao , Zhang Yirui , Huang Botao , Cai Bin , Rao Reshma , Giordano Livia , Sun Shi-Gang , Shao-Horn Yang
Published: December 2020

Proton activity at the electrified interface is central to the kinetics of proton-coupled electron transfer (PCET) reactions for making chemicals and fuels. Here we employed a library of protic ionic liquids in an interfacial layer on Pt and Au to alter local proton activity, where the intrinsic ORR activity was enhanced up to 5 times, exhibiting a volcano-shaped dependence on the pKa of the ionic liquid. The enhanced ORR activity was attributed to favorable proton transfer kinetics for strengthened hydrogen bonds between the ionic liquid to the ORR product with comparable pKa. This proposed mechanism was supported by in situ surface-enhanced Fourier-Transform Infrared Spectroscopy and our simulation of PCET kinetics based on computed proton vibrational wavefunction at the H-bond interface. These findings highlight opportunities in using non-covalent interactions of hydrogen bond structures and solvation environments at the electrified interface to tune the kinetics of ORR and beyond.
Normal & Reversed Spin Mobility in a Diradical By Electron-Vibration Coupling
Authors: Shen Yi , Xue Guodong , Dai Yasi , Quintero Sergio Moles , Chen Hanjiao , Wang Dongsheng , Miao Fang , Negri Fabrizia , Zheng Yonghao , Cordon Juan Casado
Published: December 2020

New insights on the mechanisms of spin relaxation and spin mobility in connection with the structural flexibility of π-conjugated radicals are fundamental solid-state issues in organic spintronics. Here, we describe a new azobenzene-based organic diradical with a dumbbell shape in the crystal and correlate its solid-state flexibility and its spin diradical structure. X-ray temperature-dependent studies were carried out showing two main effects on heating: i) modulation of the spin distribution and of the low-to-high spin magnetic transitions; and ii) “normal” quinoidal→aromatic transformation at low temperatures driven by the intramolecular rotational motions/mobility of the azobenzene core, and a “reversed” aromatic→quinoidal change at high temperatures activated by a bicycle pedal motion in the azobenzene unit amplified by anisotropic intermolecular interactions. Sequential versus simultaneous thermal excitation of the vibrational states associated to these motions reveal a unique and unprecedented spin-vibration mechanism.
Differentiation of Human Adipose-Derived Stem Cells into Parathyroid Hormone-Secreting Cells
Authors: Kim Eun Heui , Kim Sang Soo , Kim Ju In , Cheon Ji Min , Kim Joo Hyoung , Lee Jin Chun , Choi Kyung Un , Kim In Joo , Wang Soo Gun
Published: December 2020

IntroductionComplications arising from neck surgery are the most common causes of acquired hypoparathyroidism. Parathyroid hormone (PTH) regulates serum calcium and vitamin D levels. The current treatments for hypoparathyroidism, including PTH supplements and high doses of calcium and vitamin D, are a life-long burden and may cause side effects. Recent studies have shown that parathyroid gland differentiation occurs from embryonic stem cells, thymic epithelial cells, and tonsil-derived stem cells. Because adipose-derived stem cells (ADSCs) do not face ethical concerns raised for studies with human embryonic stem cells, in the present study, we attempted to induce differentiation of ADSCs into parathyroid cells.MethodsADSCs were isolated from abdominal subcutaneous adipose tissues obtained by plastic surgery from three male donors (mean age, 40 years). For differentiation into parathyroid-like cells, ADSCs were incubated in minimum essential medium-alpha supplemented with activin A and soluble Sonic hedgehog for 7–21 d. The parathyroid differentiation markers PTH, glial cells missing homolog 2 (GCM2), and chemokine (C-C motif) ligand 21 (CCL21) were detected using real-time quantitative polymerase chain reaction to confirm differentiation.ResultsThe ADSCs exhibited flat and vacuolated morphology, which changed into secretory parathyroid gland–like nodules on day 21 after differentiation. The mRNA expression of PTH, GCM2, and CCL21 increased in the differentiated cells. Furthermore, a significant amount of PTH protein was detected in differentiated cells on day 7 post-differentiation.ConclusionHuman ADSCs isolated from adipose tissues successfully differentiated into PTH-secreting cells. ADSC-secreted PTH may be a promising therapeutic for hypoparathyroidism patients.
Modelling of the Hungarian spread of COVID-19 and control strategies with risk-based approach
Authors: Farkas Zsuzsa , Engelhardt Tekla , Országh Erika , Süth Miklós , Csorba Szilveszter , Jóźwiak Ákos
Published: December 2020

Background

Novel Coronavirus Disease (COVID-19), caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), threatens humanity in terms of health and economy as it spreads extremely fast and causes massive epidemics all over the world. In the absence of a vaccine, social isolation and hygienic measures are the only way to curb the virus.
Methods

In our study, the Hungarian spread of COVID-19 is modelled by applying a modified SEIR (Susceptible, Exposed, Infected, Recovered) compartment model, which takes into account the route of disease transmission not only from infected, but from latent individuals (exposed compartment) as well. The differences between the modified model and the traditional SEIR model has been evaluated. The different scenarios of disease spreading simulate the effect of the different level of interventions (social distancing and hygienic measures) taken place in Hungary. The modelling also considers the population and mobility data which are also essential in case of infectious disease spreading. For controlling the disease in the long-term a network-based analysis is provided based on the concept of the epidemic threshold and the identification of super-spreader population groups.
Results

According to sensitivity analysis of the modified SEIR model, disease transmission of latent individuals has the greatest effect on the number of infections. Based on the results, the applied interventions have a great impact on the disease spreading and are effective in controlling the COVID-19 epidemic., a network-based analysis is provided based on the concept of the epidemic threshold and the identification of super-spreader population groups. According to the results of the network-based study, the proportion of people to be sampled for an effective disease control is the function of the identified people with high number of contacts in social networks who act as super-spreaders.
Conclusion

Applying network-based random, selective and targeted sampling, testing and isolation of affected individuals would yield significantly different sample sizes, highlighting the importance of super-spreaders. Network analysis (but also all computational science methods) need large amount of good quality data and the spread of these methods could be supported by easy-to-use tools. We wanted to raise awareness also on this issue.
Bronchoalveolar lavage fluid characteristics and outcomes of invasively mechanically ventilated patients with COVID-19 pneumonia in Genoa, Italy.
Authors: Dentone Chiara , Vena Antonio , Loconte Maurizio , Grillo Federica , Brunetti Iole , Barisione Emanuela , Tedone Elisabetta , Mora Sara , Biagio Antonio Di , Orsi Andrea , De Maria Andrea , Nicolini Laura , Ball Lorenzo , Giacobbe Daniele , Magnasco Laura , Delfino Emanuele , Mastracci Luca , Mangerini Rosa , Taramasso Lucia , Sepulcri Chiara , Pincino Rachele , Bavastro Martina , Cerchiaro Matteo , Malgorzata Malgorzata , Bruzzone Bianca , Icardi Giancarlo , Frisoni Paolo , Gratarola Angelo , Patroniti Niccolò , Pelosi Paolo , Bassetti Matteo
Published: December 2020

Background
To describe the cellular characteristics of bronchoalveolar lavage fluid (BALF) of critically ill COVID-19 patients requiring invasive mechanical ventilation; the secondary outcome is to describe BALF findings between survivors vs non-survivors.
Materials and Methods
Patients positive for SARS-CoV-2 RT PCR, admitted to ICU between March and April 2020 were enrolled. At ICU admission, BALF were analyzed by flow cytometry. Univariate, multivariate and Spearman correlation analyses were performed.
Results
Sixty-four patients were enrolled, median age of 64 years (IQR 58–69). The majority cells in the BALF were neutrophils (70%, IQR 37.5–90.5) and macrophages (27%, IQR 7–49) while a minority were lymphocytes, 1%, TCD3 + 92% (IQR 82–95). The ICU mortality was 32.8%. Non-survivors had a significantly older age (p = 0.033) and peripheral lymphocytes (p = 0.012) were lower compared to the survivors. At multivariate analysis the percentage of macrophages in the BALF correlated with poor outcome (OR 1.336, CI95% 1.014–1.759, p = 0.039).
Conclusions
In critically ill patients, BALF cellularity is mainly composed of neutrophils and macrophages. The macrophages percentage in the BALF at ICU admittance correlated with higher ICU mortality. The lack of lymphocytes in BALF could partly explain a reduced anti-viral response.
Evaluation of Effect of Handle Vibration Diesel-Fueled Single-Axle Tractor on Handgrip Strength of Operators
Authors: Busse Siraj , Sinclair Anthony , Wondimu Diresibachew , Reda Daniel
Published: December 2020

Background: The transmission of vibration from a single-axis tractor to the human body is determined by its dynamic response; this, in turn, depends on the physical characteristics of a person's hand, the contact area, the strength of the grip, the push force of the tractor, and the position. The purpose of this work was to measure and evaluate handgrip strength and fatigue resistance for operators of a 15 horsepower single-axle tractor before and after vibration exposure. Methods: Grip strength of dominant and non-dominant hands before and after 30 minutes of tilling operations was measured and recorded. The operators performed tilling tasks with the tractor in third gear, while the vibration levels were measured at the tractor handle along with the vertical, forward, and transverse directions. Results: The average operator grip strength was 33.6 ± 2.7 and 26.3 ± 3.3 kg and the average overall grip strength declined from 39.7 to 29.31 kg, although the average fatigue strength was 27.6 and 26.5 seconds for the dominant hand before and after vibration exposure. For the non-dominant hand, the mean grip strength measured was 28.7±2.9 and 23.1±1.9 kg and the maximum grip strength was 32.79 and 26.25, while fatigue was 29.76 and 22 seconds before and after tilling respectively.Conclusion: The average reduction in grip strength suggest considerable differences in grip strength for dominant and non-dominant hands of single axle tractor operators and shows that vibration transmitted from the single axle tractor handle has a major effect on the operators.
Anxiety and Facial Self-Contacts as Risk Factors for COVID-19 in Dental Practice.
Authors: Carrillo-Díaz María , Lacomba-Trejo Laura , Valle-Gonzalez Antonio del , Romero Martín , Gonzalez-Olmos María José
Published: December 2020

Background: This study makes innovative contributions regarding the association of facial self-contact frequency and duration in dental clinic patients with psychosocial factors such as dental fear, fear of COVID-19 and trait anxiety, because such self-contact could have an impact on the transmission of SARS-CoV-2.Results: Statistically significant associations were found between the number and duration of facial self-contacts with trait anxiety and dental fear. Women showed more frequent self-contact of the face in general and considering dental anxiety, we observe that the amount of self-contact for women remained. However, in men as dental fear increases, so do facial self-contacts. Trait anxiety is a mediating variable in the relationship between dental anxiety and the number of facial self-contacts.Conclusions: There is a positive association between the number of facial self-contacts and trait anxiety and dental anxiety. However, there is no association between self-contacts and fear of COVID-19. Women make a greater number of facial self-contacts, more trait anxiety and more dental anxiety. The relationship between dental anxiety and self-contacts was moderated by gender and the relationship between dental anxiety and self-contacts is mediated by trait anxiety.
Chronic lung diseases are associated with gene expression programs favoring SARS-CoV-2 entry and severity
Authors: Bui Linh , Winters Nichelle , Chung Mei-I , Joseph Chitra , Gutierrez Austin , Habermann Arun , Adams Taylor , Schupp Jonas , Poli Sergio , Peter Lance , Taylor Chase , Blackburn Jessica , Richmond Bradley , Nicholson Andrew , Rassl Doris , Wallace William , Rosas Ivan , Jenkins R , Kaminski Naftali , Kropski Jonathan , Banovich Nicholas
Published: December 2020

Patients with chronic lung disease (CLD) have an increased risk for severe coronavirus disease-19 (COVID-19) and poor outcomes. Here, we analyzed the transcriptomes of 605,904 single cells isolated from healthy and CLD lungs to identify molecular characteristics of lung cells that may account for worse COVID-19 outcomes in patients with chronic lung diseases. We observed a similar cellular distribution and relative expression of SARS-CoV-2 entry factors in control and CLD lungs. CLD epithelial cells expressed higher levels of genes linked directly to the efficiency of viral replication and innate immune response. Additionally, we identified basal differences in inflammatory gene expression programs that highlight how CLD alters the inflammatory microenvironment encountered upon viral exposure to the peripheral lung. Our study indicates that CLD is accompanied by changes in cell-type-specific gene expression programs that prime the lung epithelium for and influence the innate and adaptive immune responses to SARS-CoV-2 infection.
Delta-range coupling between prefrontal cortex and hippocampus supported by respiratory rhythmic input from the olfactory bulb in freely behaving rats
Authors: Mofleh Rola , Kocsis Bernat
Published: December 2020

An explosion of recent findings firmly demonstrated that brain activity and cognitive function in rodents and humans are modulated synchronously with nasal respiration. Rhythmic respiratory (RR) coupling of wide-spread forebrain activity was confirmed using advanced techniques, including current source density analysis, single unit firing, and phase modulation of local gamma activity, creating solid premise for investigating how higher networks use this mechanism in their communication. Here we show essential differences in the way prefrontal cortex (PFC) and hippocampus (HC) process the RR signal from the olfactory bulb (OB) allowing dynamic PFC-HC coupling utilizing this input. We used inter-regional coherences and their correlations in rats, breathing at low rate (~2 Hz) at rest, outside of the short sniffing bouts. We found strong and stable OB-PFC coherence, contrasting OB-HC coherence which was low but highly variable. PFC-HC coupling, however, primarily correlated with the latter, indicating that HC access to the PFC output is dynamically regulated by the responsiveness of HC to the common rhythmic drive. This pattern was present in both theta and non-theta states of waking, whereas PFC-HC communication appeared protected from RR synchronization in sleep states. The findings help to understand the mechanism of rhythmic modulation of non-olfactory cognitive processes by the on-going regular respiration, reported in rodents as well as humans. These mechanisms may also be important to understand how OB-pathology may lead to neurological consequences, similar to known olfactory disturbances in COVID-19.
The molecular mechanism of TiaoGanYiPi formula for treatment of chronic hepatitis B by network pharmacology and molecular docking verification
Authors: Cao Xu , Zao Xiaobin , Xue Baiquan , Chen Hening , Zhang Jiaxin , Li Shuo , Li Xiaobin , Zhu Shun , Guo Rui , Li Xiaoke , Ye Yongan
Published: December 2020

The Chinese herbal formula Tiao-Gan-Yi-Pi (TGYP) showed effective against Chronic Hepatitis B (CHB). In this study, we aimed to clarify the mechanisms and potential targets between TGYP and CHB through network pharmacology and molecular docking verification. The compounds of TGYP were identified in the TCMSP and CNKI databases, and their putative targets were predicted through SwissTargetPrediction and STITCH databases. The targets of CHB were obtained from the GeneCards, NCBI Gene, and DisGeNET databases. The above mentioned data were visualized using Cytoscape, and molecular docking showed the relationship between them. The expression of key targets was verified in GEO databases. Hence, we screened out 11 TGYP-related key targets for CHB included ABL1, CASP8, CCNA2, CCNB1, CDK4, CDKN1A, EP300, HIF1A, IGF1R, MAP2K1 and PGR. The key targets were predominantly enriched in the cancer, cell cycle and hepatitis B pathways and involved in the positive regulation of fibroblast proliferation, signal transduction, and negative regulation of gene expression biological processes, and expression of key target genes was related to HBV infection and liver inflammation. Through this newly constructed interaction network between TGYP and CHB, we identified active compounds and targets which could be further used for providing clinical guidance.
Hexaferrocenium Tri [Hexa(Isothiocyanato)Iron(III)] trihydroxonium Complex as a New DNA Intercalator for Electrochemical DNA Biosensor
Authors: Ariffin Eda , Zakariah Emma , Ruslin Farah , Kassim Muhammad , Yamin Bohari , Hasbullah Siti , Lee Heng
Published: December 2020

Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because of its stable thermodynamic, kinetic and redox properties. Novel Hexaferrocenium Tri[Hexa(Isothiocyanato)Iron (III)]Trihydroxonium (HexaFc) complex was the product from the reaction of ferrocene, maleic acid and ammonium thiocyanate and it was confirmed by elemental analysis CHNS, FTIR and single crystal x-ray crystallography. In this study, HexaFc was used for the first time as an electroactive indicator for DNA hybridisation biosensor. The UV-Vis DNA titrations with this compound showed hypochromism and redshift at 250 nm with increasing DNA concentrations. The binding constant (Kb) for HexaFc complex towards CT-DNA (calf-thymus DNA) was 3.1 × 104 M−1. These results indicated intercalator behaviour of the complex. To test the usefulness of this complex for DNA biosensor application, a porcine DNA biosensor was constructed. The recognition probes were covalently immobilised onto silica nanospheres (SiNS) via glutaraldehyde linker on a screen-printed electrode (SPE). After intercalation with the complex, the biosensor response to complementary porcine DNA was measured using differential pulse voltammetry. The DNA biosensor demonstrated a linear response range to the complementary porcine DNA from 1 × 10−6 to 1 × 10−3 µM (R2 = 0.9642) with a limit detection of 4.83 × 10−8 µM. The results indicated that HexaFc complex is a feasible indicator for DNA hybridisation evaluation without the need of chemical attachment as a label.
Morning chronotype decreases the risk of chemotherapy-induced peripheral neuropathy in women with breast cancer
Authors: Son Kyung-Lak , Jung Dooyoung , Lee Kwang-Min , Yeom Chan-Woo , Oh Kyu-Han , Kim Tae-Yong , Im Seock-Ah , Lee Kyung-Hun , Spiegel David , Hahm Bong-Jin
Published: December 2020

The purpose of this longitudinal prospective cohort study was to investigate the role of chronotype in the incidence of chemotherapy-induced peripheral neuropathy (CIPN) among women with breast cancer. A total of 128 subjects with breast cancer awaiting adjuvant chemotherapy without peripheral neuropathy participated in this study. The presence of CIPN was defined as a response of 3 or higher on a peripheral neuropathy subscale in the MD Anderson Symptom Inventory. Candidate psychiatric factors associated with CIPN were assessed, using the Composite Scale of Morningness, the Pittsburgh Sleep Quality Index, and the Hospital Anxiety and Depression Scale. To examine the association between chronotype and CIPN, we built logistic regression models, adjusting for demographic, clinical, and other psychiatric variables. Forty-nine participants received a chemotherapy regimen containing docetaxel, of which 29 (59%) developed CIPN. We performed subgroup analyses of docetaxel-treated participants. The morning chronotype was inversely associated with CIPN (odds ratio, 0.07; confidence interval, 0.01–0.48; p = 0.016) after adjusting for age, BMI, education, alcohol use, smoking, disease stage, sleep quality, depression, and anxiety. Our results suggest that the morning chronotype is a protective factor against the development of CIPN in patients with breast cancer who were treated with docetaxel.
Ab initio Study of the Structure, Elastic, and Electronic Properties of Ti3(Al1-nSin)C2 Layered Ternary Compounds
Authors: Ahams S. , Shaari A. , Ahmed Rashid , Pattah N. Abdul , Idris M. , Haq Bakhtiar
Published: December 2020

The MAX phase materials such as layered ternary carbides that simultaneously exhibit characteristics of metallic and ceramic materials have received substantial interest in recent years. Here, we present a systematic investigation of the electronic, structural stabilities, and elastic properties of Ti3(Al1-nSin)C2 (n = 0,1) MAX phase materials using the ab initio method via a plane-wave pseudopotential approach within generalized-gradient-approximations. The computed electronic band structures and projected density of states show that both Ti3SiC2 and Ti3AlC2 are metallic materials with a high density of states at the Fermi level emanating mainly from Ti-3d. Using the calculated elastic constants, the mechanical stability of the compounds was confirmed following the Born stability criteria for hexagonal structures. The Cauchy pressure and the Pugh’s ratio values establish the brittle nature of the Ti3SiC2 and Ti3AlC2 MAX phase materials. Due to their intriguing physical properties, these materials are expected to be suitable for applications such as thermal shock refractories and electrical contact coatings.
Catalytic Upgrading of Pyrolysis Bio-oil by Hydrotreating and Emissions Estimation of Combustion of Gas-phase Compounds 
Authors: Doukeh Rami , Bombos Dorin , Bombos Mihaela , Oprescu Elena-Emilia , Dumitrascu Gheorghe , Vasilievici Gabriel , Calin Catalina
Published: December 2020

An attractive solution for improvement of pyrolysis bio-oil composition regarding the high content of oxygenated compounds is catalytic upgrading via hydrotreating process. The objective of this work is to evaluate the catalytic activity of CoMo /γ-Al2O3-HMS in bio-oil hydrotreating. The catalyst was characterized by surface area, X-ray diffraction (XRD), TEM, FT-IR and XPS analysis. The experiments were carried out in a flow fixed-bed reactor at the temperature range of 250-320 oC, pressure between 20-40 bar, and LHSV 3h-1. The results showed that increased temperature and pressure favors the hydrodeoxygenation process, reaching the maxim bio-oil conversion of 87.23%. Also, a chemical modelling algorithm was developed in order to evaluate the harmful emissions resulted from hydrotreating gaseous phase combustion. This calculation algorithm can be applied to any pressurized or atmospheric combustion engine fueled with different mixture of combustible gases such as: natural gas, hydrogen-enriched natural gas and oxy-fuel.