bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product
Authors: de la Luz Riviello-Flores María , Román Castillo-Martínez Carlos , Jorge Cadena-Iñiguez , del Mar Ruiz-Posadas Lucero , Marcos Soto-Hernández Ramón , Lourdes Arévalo-Galarza Ma. de , Israel Castillo-Juárez
Published: May 2024

Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.
The Community of Practice: An Essential and Elegant Framework for Archaeological Interpretation
Authors: Souza Randall
Published: May 2024

Archaeologists deploy a variety of models and theories, often tailored to specific questions or situations, in making sense of the material record we study. The concept of the community of practice, originally developed in the context of modern work and learning situations, describes among other things how participation in shared activities can create and shape social relationships. It therefore offers a powerful and flexible framework for the many archaeological research agendas in which group dynamics play a role. Some archaeologists have already begun to use the community of practice approach (CoP) as an interpretive framework, and this essay argues that a wider embrace would be a benefit to individual archaeologists and to the field as a whole.
Advanced Machine Learning Techniques for Predictive Modeling of Property Prices
Authors: Mathotaarachchi Kanchana Vishwanadee , Hasan Raza , Mahmood Salman
Published: May 2024

Real estate price prediction is crucial for informed decision making in the dynamic real estate sector. In recent years, machine learning (ML) techniques have emerged as powerful tools for enhancing prediction accuracy and data-driven decision making. However, the existing literature lacks a cohesive synthesis of methodologies, findings, and research gaps in ML-based real estate price prediction. This study addresses this gap through a comprehensive literature review, examining various ML approaches, including neural networks, ensemble methods, and advanced regression techniques. We identify key research gaps, such as the limited exploration of hybrid ML-econometric models and the interpretability of ML predictions. To validate the robustness of regression models, we conduct generalization testing on an independent dataset. Results demonstrate the applicability of regression models in predicting real estate prices across diverse markets. Our findings underscore the importance of addressing research gaps to advance the field and enhance the practical applicability of ML techniques in real estate price prediction. This study contributes to a deeper understanding of ML’s role in real estate forecasting and provides insights for future research and practical implementation in the real estate industry.
Facile Synthesis of CuFe2O4 Nanoparticles for Efficient Removal of Acid Blue 113 and Malachite Green Dyes from Aqueous Media
Authors: Al-Wasidi Asma S. , Shah Reem K. , Abdelrahman Ehab A. , Mabrouk El-Sayed M.
Published: May 2024

This work studies the synthesis, characterization, and application of CuFe2O4 nanoparticles for the removal of acid blue 113 and malachite green dyes from aqueous media. Utilizing the combustion procedure, CuFe2O4 nanoparticles were synthesized using two different fuels: L-alanine (CFA) and L-valine (CFV). Besides, the synthesized CuFe2O4 nanoparticles were characterized through some tools, including Fourier transform infrared (FTIR), X-ray diffraction (XRD), energy-dispersive X-ray (EDX), and field emission scanning electron microscope (FE-SEM). XRD analysis verified the creation of a CuFe2O4 cubic spinel structure without any contaminants, revealing average crystallite sizes of 26.37 and 17.65 nm for the CFA and CFV samples, respectively. The FTIR spectra exhibited peaks indicative of metal-oxygen bond stretching, verifying the presence of a spinel formation. Elemental analysis via EDX confirmed the stoichiometric composition typical of copper ferrite. In addition, FE-SEM displayed that the CFA and CFV samples are composed of particles with spherical and irregular shapes, measuring average diameters of 188.35 and 132.78 nm, respectively. The maximum adsorption capabilities of the CFA and CFV samples towards acid blue 113 dyes are 281.69 and 297.62 mg/g, respectively. Also, the maximum adsorption capabilities of the CFA and CFV products towards malachite green dye are 280.11 and 294.99 mg/g, respectively. Kinetic and equilibrium studies revealed that the adsorption process of acid blue 113 and malachite green dyes onto the CFA and CFV samples followed the pseudo-second-order model and Langmuir isotherm. Thermodynamic analysis indicated that the adsorption process was physical, spontaneous, and exothermic.
Microbiota Analysis of Ejaculated Honey Bee Drone Semen and the Effect of Semen Collection Method on Bacterial Loads
Authors: Yániz Jesús , Toquet Marion , Santolaria Pilar , Silvestre Miguel Angel , Toledo-Perona Raquel , Gómez-Martín Ángel
Published: May 2024

Artificial insemination in queen honey bees is the only tool that provides complete control over mating for research and breeding purposes, making it essential in genetic improvement and conservation programs in this species. The aims of this study were to characterize drone semen bacterial loads by culture-dependent and independent methods and to describe their variation depending on the method of semen collection, the colony and the apiary. In the first experiment, the bacterial loads of semen collected from the seminal vesicles or from ejaculates was studied using culture-dependent methods. The collection method had a significant influence on the overall bacterial count in semen. Out of the 42 semen samples analyzed, 26 (61.9%) tested positive for bacterial isolation. This encompassed the entirety of samples obtained from the seminal vesicles (21 of 21), whereas only 23.8% of those derived from ejaculates (5 out of 21) showed bacterial isolation. In the second experiment, next-generation sequencing techniques were used to describe the microbiome of ejaculated drone semen for the first time. The most abundant phyla were Proteobacteria, Firmicutes, Bacteroidota and Actinobacteriota, while the most abundant genera were Lactobacillus, Staphylococcus, Prevotella, Alloprevotella and Streptococcus. The results showed that the apiary had a significant effect on the community structure composition and abundance of the seminal microbiota, and significative differences in abundance were observed for the genera Sphingomonas, Methylobacterium-Methylorubrum, Bifidobacterium and Alloprevotella. Significant differences were also observed in the richness of the microbiota between apiaries and colonies.
Seasonal Dynamics and Damage of Halyomorpha halys in Italian Vineyards
Authors: Scaccini Davide , Fornasiero Diego , Tirello Paola , Vincenzi Simone , Cecchetto Massimo , Allgjata Ilirjan , Duso Carlo , Pozzebon Alberto
Published: May 2024

The brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), is an invasive polyphagous pest often observed in vineyards. In Europe, a gap needs to be filled in the knowledge on H. halys seasonal dynamics and damage on grapes. With this study, we described the seasonal dynamics of H. halys and its distribution in multi-cultivar vineyards, and we evaluated the damage on grape clusters induced by different pest densities. In vineyards, the seasonal occurrence of H. halys varied across time and grape cultivars, and the pest was more abundant on Cabernet Franc, Merlot and, to a lesser extent, Pinot gris. Moreover, higher densities of H. halys were found on red berry cultivars than on white ones, and on cultivars ripening late in the season. An edge effect was also detected in pest distribution within vineyards, with more stink bugs observed in the borders. In the study on pest infestation density, H. halys caused damage on berries, showing differences in susceptibility among different cultivars and with regard to the time of infestation (i.e., plant phenological stages). Halyomorpha halys infestation induced an increase in Botrytis cinerea and sour rot incidence, which probably represents the main issue related to the impact of brown marmorated stink bug on grapevine.
Sulfuryl Fluoride Fumigation as a Quarantine Treatment for the Control of Reticulitermes speratus Kolbe (Blattodea: Rhinotermitidae) in Wood
Authors: Kim So-Yeon , Choi Na-Ra , Park Min-Goo
Published: May 2024

High-risk termites in wood imported to the Republic of Korea are currently treated with methyl bromide (MB), which has ozone-depleting properties and is highly toxic. This study evaluated the effectiveness of sulfuryl fluoride (SF) as a quarantine treatment against Reticulitermes speratus Kolbe (Blattodea: Rhinotermitidae) in wood, along with its wood sorption and penetration capacity. The LCt50 and LCt99 values for SF were 30.87 and 42.53 mg h/L at 23 °C and 151.62 and 401.9 mg h/L at 5 °C, respectively. The SF Ct values did not significantly differ between dry and wet wood at loading ratios of 10%, 30%, and 50% at both 5 °C and 23 °C (p > 0.05). In a closed wooden cube, the LCt50 and LCt99 for SF for R. speratus were 31.59 and 53.34 mg h/L, respectively, indicating an excellent wood penetration ability. SF caused 100% termite mortality with a 90% loading ratio in the scale-up trials (500 L). The SF concentration during ventilation decreased below the threshold limit value (TLV) of 5 ppm within 30 min, confirming that the working conditions were safe. This study provides a basis for the use of SF as an alternative to MB for the treatment of termites in wood.
Armoured Lepidopteran Caterpillars Preserved in Non-Fossil Resins and What They Tell Us about the Fossil Preservation of Caterpillars
Authors: Gauweiler Joshua , Amaral André P. , Haug Carolin , Haug Joachim T.
Published: May 2024

Resin is a plastic-like product of trees. Older occurrences of such resin are referred to as amber and are considered fossil resin. Younger resins are termed copals. Even younger ones have been dubbed defaunation resins. Non-fossil resins remain in a terminological limbo, often referred to as “sub-fossils”. We report two lepidopteran caterpillars preserved in non-fossil resin: one from Madagascar, one from Brazil. Prominent hairs (=setae) and spines (=spine-like setae) of the specimens make it likely that they represent larvae of Erebidae (e.g., tussock moths and others). So far, most known caterpillars preserved in resins are either “naked” or bear protective cases; only few are armoured with spines or hairs. In particular, long-haired caterpillars such as the ones reported here are so far almost absent. Only one specimen with comparable setae has been reported from 15-million-year-old Dominican amber, but no significant details of this specimen are accessible. We briefly also review the record of caterpillars known from the Holocene, recognising that it is very sparse. The new specimens demonstrate that very hairy caterpillars can readily be preserved in resins in fine detail. Furthermore, the specimens increase the known size range of caterpillars preserved in resins, with one measuring more than 12 mm.
Microparticle Hybrid Target Simulation for keV X-ray Sources
Authors: Behling Rolf , Hulme Christopher , Tolias Panagiotis , Poludniowski Gavin , Danielsson Mats
Published: May 2024

The spatiotemporal resolution of diagnostic X-ray images obtained with rotating-anode X-ray tubes has remained limited as the development of rigid, high-performance target materials has slowed down. However, novel imaging techniques using finer detector pixels and orthovolt cancer therapy employing narrow X-ray focal spots demand improved output from brilliant keV X-ray sources. Since its advent in 1929, rotating-anode technology has become the greatest bottleneck to improvement. To overcome this limitation, the current authors have devised a novel X-ray generation technology based on tungsten microparticle targets. The current study investigated a hybrid solution of a stream of fast tungsten microparticles and a rotating anode to both harvest the benefits of the improved performance of the new solution and to reuse known technology. The rotating anode captures energy that may pass a partially opaque microparticle stream and thereby contributes to X-ray generation. With reference to fast-rotating anodes and a highly appreciated small focal spot of a standardized size of 0.3 for an 8° target angle (physical: 0.45 mm × 4.67 mm), we calculated a potential output gain of at least 85% for non-melting microparticles and of 124% if melting is envisioned. Microparticle charging can be remediated by electron backscattering and electron field emission. The adoption of such a solution enables substantially improved image resolution.
Pneumococcal and Influenza Vaccination Coverage in Patients with Heart Failure: A Systematic Review
Authors: Papagiannis Dimitrios , Kourek Christos , Briasoulis Alexandros , Fradelos Evangelos C. , Papagianni Evangelia D. , Papadimopoulos Ilias , Giamouzis Grigorios , Skoularigis John , Xanthopoulos Andrew
Published: May 2024

Background/Objectives: As heart failure (HF) patients face increased vulnerability to respiratory infections, optimizing pneumococcal and influenza vaccination coverage becomes pivotal for mitigating additional health risks and reducing hospitalizations, morbidity, and mortality rates within this population. In this specific subpopulation of patients, vaccination coverage for pneumococcal and influenza holds heightened significance compared to other vaccines due to their susceptibility to respiratory infections, which can exacerbate existing cardiovascular conditions and lead to severe complications or even death. However, despite the recognized benefits, vaccination coverage among HF patients remains below expectations. The aim of the present systematic review was to assess the vaccination coverage for influenza and pneumococcus in HF patients from 2005 to 2023 and the vaccination’s effects on survival and hospitalizations. Methods: The authors developed the protocol of the review in accordance with the PRISMA guidelines, and the search was performed in databases including PubMed and Scopus. After the initial search, 851 studies were found in PubMed Library and 1961 in Scopus (total of 2812 studies). Results: After the initial evaluation, 23 publications were finally included in the analysis. The total study population consisted of 6,093,497 participants. Regarding the influenza vaccine, vaccination coverage ranged from low rates of 2.5% to very high rates of 97%, while the respective pneumococcal vaccination coverage ranged from 20% to 84.6%. Most studies demonstrated a beneficial effect of vaccination on survival and hospitalizations. Conclusions: The present systematic review study showed a wide variety of vaccination coverage among patients with heart failure.
The Diagnostic Utility of Biochemical Markers and Intestinal Ultrasound Compared with Endoscopy in Patients with Crohn’s Disease and Ulcerative Colitis: A Systemic Review and Meta-Analysis
Authors: Huynh David , Rubtsov Denis , Basu Debapama , Khaing Myat Myat
Published: May 2024

Background: Inflammatory bowel disease (IBD) consists of Crohn’s disease (CD) and Ulcerative colitis (UC). The main goal of treatment is to obtain mucosal healing via endoscopy. More recently, intestinal ultrasounds, along with biochemical markers, have been increasingly popular as point-of-care testing to monitor treatment response. This systemic review and meta-analysis aimed to assess the diagnostic test performance of ultrasonography and biochemical markers (C-reactive protein and fecal calprotectin) compared with endoscopy for detecting inflammation in IBD. Methods: A comprehensive literature search was conducted using PubMed Medline, EMBASE, ScienceDirect, and CINAHL from 1 January 2018 to 1 January 2024. The included studies were prospective and retrospective observational studies, clinical trials, and cross-sectional studies investigating the diagnostic sensitivity and specificity of ultrasonography, biochemical markers, and endoscopy. Studies were selected based on the Preferred Reporting Items for Systematic Review and Meta-analysis Statement (PRISMA). Results: Of the 1035 studies retrieved, 16 met the inclusion criteria, and most of the included studies were prospective observational studies. Diagnostic test accuracy was conducted, and the pooled sensitivity and specificity of all the studies revealed that ultrasonography has the highest pooled sensitivity, at 85% (95% CI, 78 to 91%), and specificity, at 92% (95% CI, 86 to 96%), as compared with biochemical markers and endoscopy. More specifically, biochemical markers had a pooled sensitivity and specificity of 85% (95% CI, 81 to 87%) and 61% (95% CI, 58 to 64%), respectively, and endoscopy had 60% (95% CI, 52 to 68%) and 82% (95% CI, 76 to 87%), respectively. However, the results also show substantial heterogeneity in the studies because of various populations, protocols, and outcomes in the studies included. This was especially noted in the assessment of biochemical markers, in which a metaregression was performed showing a nonsignificant p-value of 0.8856 for the coefficient. Conclusions: IUS was found to have the highest pooled sensitivity and specificity of all the included studies for diagnosing inflammation in patients with CD and UC, and this, coupled with biochemical markers, can improve diagnostic utility.
Injection of Autologous Adipose Stromal Vascular Fraction in Combination with Autologous Conditioned Plasma for the Treatment of Advanced Knee Osteoarthritis Significantly Improves Clinical Symptoms
Authors: Klingenberg Markus , Dineva Antoniya , Hoyer Annika , Kaltschmidt Barbara , Leimkühler Philipp , Vordemvenne Thomas , Elsner Andreas , Wähnert Dirk
Published: May 2024

(1) Background: Osteoarthritis (OA) is the most common joint disease in the world. It is chronic, systemic, progressive and disabling. Orthobiologics have the potential to positively alter the course of this disease. Therefore, the aim of this study is to evaluate the efficacy of SVF/ACP in the treatment of advanced osteoarthritis of the knee in an unfiltered patient population. We hypothesize that this therapy can improve the symptoms associated with osteoarthritis of the knee. We also hypothesize that there are patient-related factors that influence the efficacy of therapy. (2) Methods: Two hundred and thirteen patients with moderate to severe OA of the knee and SVF/ACP injection were recruited for this study. Patients were excluded if they did not provide informed consent or were not receiving SVF/ACP therapy. Pain, function, symptoms and quality of life were assessed using standardized scores (KOOS, WOMAC) before and after treatment. (3) Results: The VAS pain score was significantly reduced by at least 30% (p < 0.001). Knee function, as measured by the KOOS daily activity and sport scores, showed significant increases of 21% and 45%, respectively, at 6 months (p < 0.04). (4) Conclusions: Treatment of knee OA with SVF/ACP injection positively modifies the disease by significantly reducing pain and improving function.
Potential of Titanium Pins Coated with Fibroblast Growth Factor-2–Calcium Phosphate Composite Layers to Reduce the Risk of Impaired Bone–Pin Interface Strength in the External Fixation of Distal Radius Fractures
Authors: Mutsuzaki Hirotaka , Yanagisawa Yohei , Noguchi Hiroshi , Ito Atsuo , Yamazaki Masashi
Published: May 2024

Background: The risk of impaired bone–pin interface strength in titanium (Ti) pins coated with fibroblast growth factor (FGF)–calcium phosphate (CP) composite layers is yet to be evaluated in a clinical study. This retrospective study used Weibull plot analysis to evaluate bone–pin interface strength in Ti pins coated with FGF-CP layers for external distal radius fracture fixation. Methods: The distal radial fractures were treated with external fixation. The FGF-CP group comprised five patients (all women, aged 70.4 ± 5.9 (range: 62–77) years), and the uncoated pin group comprised ten patients (eight women and two men, aged 64.4 ± 11.7 (range: 43–83) years). The pins were removed after six weeks. The insertion and extraction peak torques were measured. The extraction peak torque was evaluated using Weibull plot analysis. Results: We compared the extraction torque of the two groups at or below 506 Nmm for a fair comparison using Weibull plot analysis. The Weibull plots were linear for both the FGF-CP and uncoated pin groups. The slope of the regression line was significantly higher in the FGF-CP group (1.7343) than in the uncoated pin group (1.5670) (p = 0.011). The intercept of the regression line was significantly lower in the FGF-CP group (−9.847) than in the uncoated pin group (−8.708) (p = 0.002). Thus, the two regression lines significantly differed. Conclusions: Ti pins coated with FGF-CP layers exhibit the potential to reduce the risk of impaired bone–pin interface strength in the external fixation of distal radius fractures.
Clinical and Surgical Applications of Large Language Models: A Systematic Review
Authors: Pressman Sophia M. , Borna Sahar , Gomez-Cabello Cesar A. , Haider Syed Ali , Haider Clifton R. , Forte Antonio Jorge
Published: May 2024

Background: Large language models (LLMs) represent a recent advancement in artificial intelligence with medical applications across various healthcare domains. The objective of this review is to highlight how LLMs can be utilized by clinicians and surgeons in their everyday practice. Methods: A systematic review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Six databases were searched to identify relevant articles. Eligibility criteria emphasized articles focused primarily on clinical and surgical applications of LLMs. Results: The literature search yielded 333 results, with 34 meeting eligibility criteria. All articles were from 2023. There were 14 original research articles, four letters, one interview, and 15 review articles. These articles covered a wide variety of medical specialties, including various surgical subspecialties. Conclusions: LLMs have the potential to enhance healthcare delivery. In clinical settings, LLMs can assist in diagnosis, treatment guidance, patient triage, physician knowledge augmentation, and administrative tasks. In surgical settings, LLMs can assist surgeons with documentation, surgical planning, and intraoperative guidance. However, addressing their limitations and concerns, particularly those related to accuracy and biases, is crucial. LLMs should be viewed as tools to complement, not replace, the expertise of healthcare professionals.
Diastolic Heart Failure Mechanisms and Assessment Revisited
Authors: Pai Ramdas G. , Varadarajan Padmini
Published: May 2024

The syndrome of heart failure (HF) with preserved ejection fraction (HFpEF) makes up about half of the HF population. The HF mechanisms in these patients are varied and not fully understood. In addition, the term “diastolic HF” was switched to HFpEF because of difficulties in measuring the left ventricular (LV) diastolic performance. In the late stages, HFpEF carries a prognosis that is as bad as or worse than that of HFrEF. Hence, it is important to recognize LV diastolic impairment at an earlier stage so that the causal mechanisms, if any, can be treated to retard its progression. Despite the availability of numerous disease-modifying agents for HFrEF, there are hardly any available treatments for HFpEF. With our aging population, there will be an epidemic of HFpEF and hence this entity needs attention and respect. In this paper, we review the fundamental mechanisms of HFpEF, the physiology of LV filling and how LV diastolic function can be comprehensively measured. We also speculate how this may help with the early recognition of diastolic HF and its treatment.
Role of Complement Components in Asthma: A Systematic Review
Authors: Tornyi Ilona , Horváth Ildikó
Published: May 2024

Background: Asthma is a chronic inflammatory airway disease characterized by recurrent symptoms in response to a wide range of external stimuli, including allergens, viral infections, and air pollution together with internal host-derived danger signals. The disease is traditionally associated with adaptive immune responses; recent research emphasizes the critical role of innate immunity in its pathogenesis. The complement system, activated as part of the defense mechanisms, plays a crucial role in bridging innate to adaptive immunity. While experimental models demonstrate complement cascade activation in asthma, human studies remain limited. Methods: This systematic review summarizes existing literature on the complement system in asthma patients, gathering data from PubMed, Web of Science, Scopus, and Google Scholar. The protocol was registered in the OSF. Results: Out of 482 initially identified articles, only 24 met the eligibility criteria, revealing disparities in sample origin, methodologies, and populations. Despite observed heterogeneity, a consistent result was found in the elevation of complement regulatory proteins, such as complement Factor H, in samples from patients with asthma compared to those from healthy subjects. Conclusions: The increased level of regulatory proteins, such as Factor H and I highlight that these may influence asthma pathophysiology. The role of complement factors as potential biomarkers of asthma activity and severity needs further evaluation.
Adherence to Screening Tests for Gynaecological and Colorectal Cancer in Patients with Diabetes in Spain: A Population-Based Study (2014–2020)
Authors: Zeng-Zhang Luyi , de Miguel-Diez Javier , López-de-Andrés Ana , Jiménez-García Rodrigo , Ji Zichen , Meizoso-Pita Olalla , Sevillano-Collantes Cristina , Zamorano-León Jose J.
Published: May 2024

Objectives: Both diabetes mellitus (DM) and gynaecological and colorectal cancers are highly prevalent diseases. Furthermore, the presence of DM constitutes a risk factor and poor prognostic indicator for these types of cancer. This study is based on the European Health Interview Surveys in Spain (EHISS) of 2014 and 2020. It aimed to determine the trends in adherence to screening tests for gynaecological cancers (breast and cervical) and colorectal cancer, compare adherence levels between populations with and without diabetes, and identify predictors of adherence in the population with diabetes. Methods: An epidemiological case-control study based on the EHISS data of 2014 and 2020 was conducted. The characteristics of participants who underwent screening tests were analysed based on the presence or absence of DM, and predictors of adherence to these preventive activities were identified. Results: A total of 1852 participants with reported DM and 1852 controls without DM, adjusted for age and sex, were included. A higher adherence to mammography was observed in women without diabetes compared to those with diabetes, although statistical significance was not reached (72.9% vs. 68.6%, p = 0.068). Similarly, higher Pap smear adherence was observed in the population without diabetes in the age group between 60 and 69 years compared to the population with diabetes (54.0% vs. 45.8%, p = 0.016). Pap smear adherence among women with diabetes was significantly higher in the EHISS of 2020 (52.0% in 2014 vs. 61.0% in 2020, p = 0.010), as was the case for faecal occult blood testing (13.8% in 2014 vs. 33.8% in 2020, p < 0.001), but it was not significant for mammography (70.4% in 2014 vs. 66.8% in 2020, p = 0.301). Overall, the predictors of adherence to screening tests were older age, history of cancer and higher education level. Conclusions: Adherence levels to cancer screening tests were lower in the population with diabetes compared to those without diabetes, although an improvement in Pap smear and faecal occult blood test adherence was observed in 2020 compared to 2014. Understanding predictors is important to improve adherence rates in the population with diabetes.
Effect of Calcium Silicate-Based Intracanal Medicament and Calcium Hydroxide on Growth Factor TGF-β1 Release from Root Canal Dentine
Authors: Bilvinaite Goda , Drukteinis Saulius , Simoliunas Egidijus , Widbiller Matthias , Sakirzanovas Simas
Published: May 2024

Effective root canal disinfection and the subsequent release of natural growth factors from dentin are crucial to the success of regenerative endodontic procedures. This study evaluated the effect of newly introduced calcium silicate-based temporary intracanal medicament Bio-C Temp and calcium hydroxide-based material UltraCal XS on the release of transforming growth factor β1 (TGF-β1) from root canal dentin. Twenty-two intact and fully developed human premolars from patients aged 15–18 were shaped and irrigated according to the current clinical recommendations. The teeth were then gently split in half, and the root canal dentin of paired samples was covered with Bio-C Temp or UltraCal XS. After 3 weeks of incubation, the specimens were conditioned with 17% EDTA and the collected solution was subjected to the quantification of the released TGF-β1 by performing an ELISA. One-way analysis of variance (ANOVA), followed by Tukey’s test, was selected to determine the statistically significant differences between the groups at the 0.95 confidence level. The highest mean value of released TGF-β1 (1993.1 pg/mL) was detected in the control group, where the root canal dentin was conditioned with 17% EDTA alone. Regarding the experimental groups, Bio-C Temp released a statistically significantly higher amount of TGF-β1 (282.14 pg/mL) compared to UltraCal XS (114.28 pg/mL; p = 0.0158). Bio-C Temp affected the release of growth factors from root canal dentin less than UltraCal XS and may therefore serve as an intracanal medicament for regenerative endodontic procedures.
Lean Body Mass, Muscle Architecture and Powerlifting Performance during Preseason and in Competition
Authors: Tromaras Konstantinos , Zaras Nikolaos , Stasinaki Angeliki-Nikoletta , Mpampoulis Thomas , Terzis Gerasimos
Published: May 2024

Lean body mass (LBM) is correlated with powerlifting performance in athletes competing in different bodyweight classes. However, it remains unknown whether changes in LBM are correlated with performance changes in powerlifters preparing for a competition. The aim of this study was to investigate the changes in LBM and performance in powerlifters preparing for a competition. Eight male powerlifters (age 31.7 ± 9.8 years, height 1.77 ± 0.06 m, weight 99.2 ± 14.6 kg) and three female powerlifters (age 32.7 ± 16.3 years, height 1.54 ± 0.06 m, weight 66.6 ± 20.9 kg) participated in the study. The athletes followed individualized periodized training programs for 12 weeks, aiming to maximize their performance for the national championship. The maximum strength (1-RM) in the squat, bench press, and deadlift, body composition, handgrip strength, anaerobic power, quadriceps’ cross-sectional area and vastus lateralis muscle architecture were measured before and after the training period. Significant increases were found after the training period in the squat (5.8 ± 7.0%, p < 0.05), bench press (4.9 ± 9.8%, p = 0.05) and deadlift (8.3 ± 16.7%, p < 0.05). Significant correlations were found between the 1-RM and LBM before and after the training period (r > 0.75, p < 0.05). The changes in the 1-RM after the training intervention correlated with the changes in the total LBM (p < 0.05). These results suggest that individual changes in LBM due to systematic resistance training for a competition may dictate increases in the 1-RM strength in powerlifters.
A Comparison of Bioelectric and Biomechanical EMG Normalization Techniques in Healthy Older and Young Adults during Walking Gait
Authors: Commandeur Drew , Klimstra Marc , Brodie Ryan , Hundza Sandra
Published: May 2024

This study compares biomechanical and bioelectric electromyography (EMG) normalization techniques across disparate age cohorts during walking to assess the impact of normalization methods on the functional interpretation of EMG data. The biomechanical method involved scaling EMG to a target absolute torque (EMGTS) from a joint-specific task and the chosen bioelectric methods were peak and mean normalization taken from the EMG signal during gait, referred to as dynamic mean and dynamic peak normalization (EMGMean and EMGPeak). The effects of normalization on EMG amplitude, activation pattern, and inter-subject variability were compared between disparate cohorts, including OLD (76.6 yrs N = 12) and YOUNG (26.6 yrs N = 12), in five lower-limb muscles. EMGPeak normalization resulted in differences between YOUNG and OLD cohorts in Biceps Femoris (BF) and Medial Gastrocnemius (MG) that were not observed with EMGMean or EMGTS normalization. EMGPeak and EMGMean normalization also demonstrated interactions between age and the phase of gait in BF that were not seen with EMGTS. Correlations showed that activation patterns across the gait cycle were similar between all methods for both age groups and the coefficient of variation comparisons found that EMGTS produced the greatest inter-subject variability. We have shown that the normalization technique can influence the interpretation of findings when comparing disparate populations, highlighting the need to carefully interpret functional differences in EMG between disparate cohorts.
Fine-Grained Food Image Recognition: A Study on Optimising Convolutional Neural Networks for Improved Performance
Authors: Boyd Liam , Nnamoko Nonso , Lopes Ricardo
Published: May 2024

Addressing the pressing issue of food waste is vital for environmental sustainability and resource conservation. While computer vision has been widely used in food waste reduction research, existing food image datasets are typically aggregated into broad categories (e.g., fruits, meat, dairy, etc.) rather than the fine-grained singular food items required for this research. The aim of this study is to develop a model capable of identifying individual food items to be integrated into a mobile application that allows users to photograph their food items, identify them, and offer suggestions for recipes. This research bridges the gap in available datasets and contributes to a more fine-grained approach to utilising existing technology for food waste reduction, emphasising both environmental and research significance. This study evaluates various (n = 7) convolutional neural network architectures for multi-class food image classification, emphasising the nuanced impact of parameter tuning to identify the most effective configurations. The experiments were conducted with a custom dataset comprising 41,949 food images categorised into 20 food item classes. Performance evaluation was based on accuracy and loss. DenseNet architecture emerged as the top-performing out of the seven examined, establishing a baseline performance (training accuracy = 0.74, training loss = 1.25, validation accuracy = 0.68, and validation loss = 2.89) on a predetermined set of parameters, including the RMSProp optimiser, ReLU activation function, ‘0.5’ dropout rate, and a 160×160 image size. Subsequent parameter tuning involved a comprehensive exploration, considering six optimisers, four image sizes, two dropout rates, and five activation functions. The results show the superior generalisation capabilities of the optimised DenseNet, showcasing performance improvements over the established baseline across key metrics. Specifically, the optimised model demonstrated a training accuracy of 0.99, a training loss of 0.01, a validation accuracy of 0.79, and a validation loss of 0.92, highlighting its improved performance compared to the baseline configuration. The optimal DenseNet has been integrated into a mobile application called FridgeSnap, designed to recognise food items and suggest possible recipes to users, thus contributing to the broader mission of minimising food waste.
Desalination in Spain and the Role of Solar Photovoltaic Energy
Authors: Martínez-Medina Miquel Àngel , Pérez-Martín Miguel Ángel , Estrela Teodoro
Published: May 2024

Water scarcity will increase in the world in the coming decades due to climate change, especially in areas that currently already have water scarcity, such as the Mediterranean area. In these areas, to guarantee water resources, systems’ sustainability is necessary to improve demand management and the development of non-conventional resources, such as treated wastewater reuse or seawater desalination. These non-conventional resources are highly energy-consuming; so, reducing energy costs is a key element in developing their use in different sectors, including agriculture. Combining photovoltaic solar energy with seawater desalination by reverse osmosis will reduce the cost of producing water to below 0.36 EUR/m3; so, this resource can be attractive for agriculture, as demonstrated in this work. The arrangement of bifacial solar modules in horizontal single-axis tracking systems increases the energy amount generated from the sun in one hour or more, improving the facility’s efficiency and reducing the desalinated water cost. The greater distance between the solar module lines, with a ground coverage ratio (GCR) = 0.3, makes for a better environmental integration of the facility and allows the development of agrovoltaic strategies, such as native flora planting and pollinator colonization.
Special Issue “Cancer Biomarker Research and Personalized Medicine 2.0”
Authors: Meehan James , Gray Mark
Published: May 2024

In 2022, there was an estimated incidence of 20 million cancer cases and 9 [...]
Ultrasonographic Insights into Peripheral Psoriatic Arthritis: Updates in Diagnosis and Monitoring
Authors: Bonfiglioli Karina Rossi , Lopes Fernanda Oliveira de Andrade , Figueiredo Letícia Queiroga de , Ferrari Luis Fernando Fernandes , Guedes Lissiane
Published: May 2024

Psoriatic arthritis (PsA) is a chronic inflammatory arthritis associated with psoriasis, characterized by heterogeneous clinical manifestations and variable disease progression. Ultrasonography has emerged as a valuable tool in the diagnosis and monitoring of PsA, providing real-time visualization of joint and soft tissue abnormalities. This review highlights recent advancements in ultrasonographic techniques for the assessment of PsA, including the identification of typical features, the role of power Doppler imaging in detecting active inflammation, and the potential of ultrasound for guiding treatment decisions. Additionally, we discuss the utility of ultrasound in assessing treatment response and monitoring disease progression in patients with PsA, with a focus on novel imaging modalities. By elucidating the evolving role of ultrasonography in PsA management, this article aims to enhance clinicians’ understanding of its utility in facilitating early diagnosis, optimizing treatment strategies, and improving patient outcomes.
Intravascular Laser Blood Irradiation (ILIB) Enhances Antioxidant Activity and Energy Metabolism in Aging Ovaries
Authors: Lin Li-Te , Li Chia-Jung , Chern Chyi-Uei , Lin Pei-Hsuan , Lin Po-Wen , Chen Yu-Chen , Tsai Hsiao-Wen , Tsui Kuan-Hao
Published: May 2024

Background: Ovarian aging is characterized by the accumulation of free radicals, leading to tissue damage and affecting reproductive health. Intravascular laser irradiation of blood (ILIB, using a low-energy He-Ne laser) is known for its efficacy in treating vascular-related diseases by reducing free radicals and inflammation. However, its impact on ovarian aging remains unexplored. This study aimed to investigate the effects of ILIB on oxidative stress and energy metabolism in aging ovaries. Methods: Genetic analysis was conducted on 75 infertile patients with aging ovaries, divided into ILIB-treated and control (CTRL) groups. Patients underwent two courses of laser treatment, and clinical parameters were evaluated. Cumulus cells were collected for the genetic analysis of oxeiptosis, glycolysis, and the tricarboxylic acid (TCA) cycle. Results: The analysis of gene expression patterns revealed intriguing findings in ILIB-treated patients compared to the untreated group. Notably, ILIB treatment resulted in significant upregulation of oxeiptosis-related genes AIFM1 and NRF2, suggesting a potential protective effect against oxidative stress-induced cell death. Furthermore, ILIB treatment led to a downregulation of glycolysis-associated gene hexokinase 2 (HK2), indicating a shift away from anaerobic metabolism, along with an increase in PDHA levels, indicative of enhanced mitochondrial function. Consistent with these changes, ILIB-treated patients exhibited elevated expression of the key TCA cycle genes citrate synthase (CS), succinate dehydrogenase complex subunit A (SDHA), and fumarate hydratase (FH), signifying improved energy metabolism. Conclusion: The findings from this study underscore the potential of ILIB as a therapeutic strategy for mitigating ovarian aging. By targeting oxidative stress and enhancing energy metabolism, ILIB holds promise for preserving ovarian function and reproductive health in aging individuals. Further research is warranted to elucidate the underlying mechanisms and optimize the application of ILIB in clinical settings, with the ultimate goal of improving fertility outcomes in women experiencing age-related ovarian decline.
Quality and Establishment of Some Water-Conserving Turfgrass Species for Sustainable Development and Some Ecosystem Services in Arid Urban Environments
Authors: Kazemi Fatemeh , Golzarian Mahmood Reza , Rabbani Kheir Khah Seyedeh Maliheh
Published: May 2024

Turfgrasses are essential landscape plants with social, environmental, and aesthetic services for urban ecosystems. However, more is needed to know how to establish them so that they can benefit from their ecosystem services in urban environments. This research examined some quality and morphological and physiological factors for the establishment and social and environmental service assessment of three warm-season turfgrasses, including Kikuyu grass (Pennisetum clandestinum), bermuda grass (Cynodon dactylon), and buffalo grass (Buchloe dactyloides), compared to the cool-season grass of tall fescue (Festuca arundinacea Schreb.). The experiment was split-plot in time, based on a randomized complete block design with eight replications. The main plot was the season with four levels, and the subplot was the four turfgrass species types. The results indicated that seasons and turfgrass types and their interaction significantly impacted most measured variables (p ≤ 0.01). Some quality measurements like turf density, color, texture, coverage, and quality after clipping and establishment confirmed the superiority of Buchloe dactyloides over the other species. Also, kikuyu grass showed higher turfgrass density, more potential for weed control, and higher coverage and growth rate but also showed invasiveness features. Tall fescue had the lowest visual aesthetic compared with the other turfgrass species. Warm-season turfgrasses adaptable to the ecology of the region should be used compared to tall fescue to achieve better turfgrass quality and social and ecosystem services for the sustainable development of arid urban environments.
A Review on the Use of Geodesign Processes in Managing Flood Vulnerability
Authors: Pang Bo , Deal Brian
Published: May 2024

The integration of geodesign with vulnerability analysis offers a novel approach to landscape, urban, and environmental planning challenges. The interdisciplinary nature, engaged processes, and advanced spatial analytics in geodesign provide important component pieces for identifying and addressing vulnerable places. A comparative case-study approach explores the geodesign process and its efficacy in addressing flooding issues in diverse settings. The objectives of this review include assessing geodesign processes in the development of vulnerability indices and assessments, exploring their effectiveness and limitations, and providing insights across different scales and contexts. The work seeks to contribute to the advancement of vulnerability assessment methodologies and their broader role in the fields of landscape design and urban and environmental planning. The findings of this study are expected to provide insights for researchers and practitioners looking to produce more effective, sustainable, and resilient planning and design outcomes.
Impact of Land Use and Climate Change on Streamflow: An Assessment Using a Semi-Empirical Model in the Guishui Watershed of North China
Authors: Gao Chunni , Honti Mark , Cheng Jinhua , Wang Tao
Published: May 2024

Land-use change may significantly influence streamflow. The semi-empirical model PhosFate was used to analyze the impact of land use and climate change on streamflow by choosing the Guishui watershed as a pilot site and then expanding, applying it to all of North China. The Guishui watershed (North Beijing, China) has experienced a dramatic decline in its streamflow in recent decades. Parallel to this, significant land-use change has happened in this area; afforestation programs have increased forest cover from 41% (1980) to 59% (2013) and a similar increase in forest cover can also be observed in North China. Managing flow decline requires separating climatic and direct human-influenced effects. The results showed the following: (1) Afforestation is a major factor that decreased total flow in the Guishui watershed from 1996 to 2014; total flow increased by around 24% more than the actual dataset in the constant scenario (no afforestation) and decreased by 5% more than the actual dataset in the forest scenario (all agriculture land use transferred to forests). (2) When forest coverage increases, the Qinghai–Tibet Plateau and the Loess Plateau are the most sensitive areas regarding total flow in North China; the total flow change rate increased by up to 25% in these two areas when land use shifted from sparse vegetation to mixed forests. After analyzing the contributions of these two factors, we formulated recommendations on future afforestation practices for North China. In the central–north and northwest districts, the annual precipitation is under 520 mm and 790 mm, respectively, and the practice of afforestation should be more carefully planned to prevent severe damage to streams. This research also proved that the PhosFate model can be used in North China, which would be a practical tool for watershed management.