bluuebunny/crossref_metadata_2025 huggingface dataset:
Adolescent Search for Birthparents
Authors: Wrobel Gretchen Miller , Grotevant Harold D. , McRoy Ruth G.
Published: May 2004

The decision to search for birthparents is one that all adopted persons consider. The focus of this study is to describe, for a group of adopted adolescents, who chooses to search and who does not and to explore how search behavior is related to the functioning of the adolescents’adoptive families and adolescents’psychological adjustment. Participants in the study included 93 adolescents whose contact with birthparents ranged from no contact or information about birthparents to those who had some information but no direct contact with birthparents. Adolescents who indicated no desire to search for their birthparents and those who indicated a desire to search or had searched were included in the study. Older adolescents who experienced some openness in their adoption, were the least satisfied with that adoptive openness, and were preoccupied with their adoptive status were most likely to search. Search behavior was not related to family functioning or adolescent problem behavior.
Relations Between Social Self-Perceptions, Time Use, and Prosocial or Problem Behaviors During Adolescence
Authors: Jacobs Janis E. , Vernon Margaret K. , Eccles Jacquelynne S.
Published: May 2004

This study investigated the relations between social self-perceptions, time use, and later involvement in prosocial or problem behaviors during early, middle, and later adolescence. The authors used an idiographic approach to identify four different patterns of social self-perceptions (confident, anxious, unconcerned, desperate) and then examined the relations between group membership and time use. As predicted, social selfperceptions were significantly related to the ways in which adolescents spend their time and to later involvement in later prosocial and problem behaviors. Notably, adolescents who were most self-confident about their social skills (Confident group) and those who were most willing to go to great lengths to make and keep friends (Desperate group) spent significantly more time with peers than those in the other two groups. In addition, patterns of social self-perceptions were significantly related to adolescents’ own involvement and their perceptions of friends’involvement in prosocial and problembehaviors.
Harm Reduction for the Prevention of Youth Gambling Problems
Authors: Dickson Laurie M. , Derevensky Jeffrey L. , Gupta Rina
Published: May 2004

Despite the growing popularity of the harm reduction approach in the field of adolescent alcohol and substance abuse, a harm reduction approach to prevention and treatment of youth problem gambling remains largely unexplored. This article poses the question of whether the harm reduction paradigm is a promising approach to the prevention of adolescent problem gambling and other risky behaviors. The authors use a universal, selective, and indicative prevention framework to present current prevention initiatives that have emerged from the harm reduction health paradigm for adolescent substance and alcohol abuse. The risk-protective factor model is used as a conceptual basis for designing youth problem gambling harm reduction prevention programs. This framework illustrates the developmental appropriateness of the harm reduction approach for youth. Implications drawn from this conceptual examination of harm reduction as a prevention approach to adolescent problem gambling provide valuable information for treatment providers as well.
Posttraumatic Growth among Adolescents
Authors: Milam Joel E. , Ritt-Olson Anamara , Unger Jennifer B.
Published: May 2004

This study examined posttraumatic growth (PTG), the construing of benefits from a traumatic event, in a sample of 435 mostly Hispanic adolescentswho experienced a major life event within the previous 3 years. Levels of PTG did not differ between experienced negative life events. The relationship between PTG and sociodemographics, substance use, religiosity, and depression was explored. Age and religiosity were positively associated with PTG, whereas substance use was inversely associated with PTG. Only age and substance use remained associated with PTG after adjusting for all other variables in a multiple regression analysis. These results demonstrate the existence of PTG among an adolescent population and suggest that PTG can be related to important health behaviors such as substance use.
Socioeconomic Adversity, Educational Resilience, and Subsequent Levels of Adult Adaptation
Authors: Schoon Ingrid , Parsons Samantha , Sacker Amanda
Published: May 2004

The aim of this article is to determine the extent to which individual, family, and contextual resources influence the school adjustment of 16-year-old teenagers and to investigate their consequent adult attainments at age 33. Adopting a longitudinal perspective, the experiences of more than 9,000 socially advantaged and disadvantaged young people are compared. The study shows that socioeconomic adversity is a significant risk factor for educational failure and that it influences consequent adjustment in work and healthrelated outcomes. Various social-psychological factors can counterbalance such adversity. In particular, parental educational aspirations for their child are significantly associated with educational resilience among less privileged individuals. The study confirms the long-term stability of secondary school adjustment. It is concluded that the factors and processes that modify the impact of adversity are context specific and that their influences have to be studied in the context in which they operate.
Responses of the Circadian System of Rats to Conditioned and Unconditioned Stimuli
Authors: de Groot Marleen H. M. , Rusak Benjamin
Published: May 2004

The circadian systems of rodents respond to light pulses presented during the subjective night with phase shifts and altered cellular activity in the suprachiasmatic nuclei (SCN), including expression of immediate-early genes (IEGs) such as c-fos. Arecent study showed that a nonphotic stimulus (an air disturbance generated by a fan) that does not normally induce the expression of c-fos-like immunoreactivity in the SCN of rats can be made to do so after being paired repeatedly with a light pulse in a Pavlovian conditioning paradigm. Furthermore, after conditioning (but not after noncontingent exposure to these stimuli), the fan also induced phase shifts in activity and body temperature rhythms comparable to those produced by light. The authors performed three experiments designed to replicate and extend these findings in rats. In experiment 1, rats were tested for conditioning effects of repeated pairings of a light pulse with a neutral air disturbance under a full photoperiod. In experiment 2, a modified conditioning paradigm was used in which a skeleton photoperiod served as both the entraining zeitgeber and the unconditioned stimulus. Animals in the paired and unpaired training conditions were exposed to both the light pulse and the air disturbance, but the air disturbance signaled the onset of light in the paired condition only. Phase shifts of wheel-running activity rhythms and gene expression in the SCN, intergeniculate leaflet, and paraventricular nucleus of the thalamus were assessed in animals following either of the training conditions or the control procedures. Experiment 3 assessed whether the air disturbance could entrain the circadian activity rhythms of rats with or without previous pairing with light in a classical conditioning paradigm. No evidence for classical conditioning, nor for unconditioned effects of the air disturbance on the circadian system, was found in these studies.
Photoperiod and Temperature Interact to Affect the GnRH Neuronal System of Male Prairie Voles (Microtus ochrogaster)
Authors: Kriegsfeld Lance J. , Ranalli Nathan J. , Bober Marie A. , Nelson Randy J.
Published: May 2004

Individuals of numerous species limit energy expenditure during winter by inhibiting reproduction and other nonessential functions. To time these adaptations appropriately with the annual cycle, animals rely on environmental cues that predict, well in advance, the onset of winter. The most commonly studied environmental factor that animals use to time reproduction is photoperiod. Rodents housed in short photoperiods in the laboratory or in naturally declining day lengths exhibit pronounced alterations in reproductive function concomitant with alterations in the hypothalamic gonadotropin-releasing hormone neuronal system. Because animals in their natural environment use factors in addition to photoperiod to time reproduction, the present study sought to determine the independent effects of photoperiod and temperature, as well as the interaction between these factors, on reproductive parameters and the GnRH neuronal system. Male prairie voles were housed in either long (LD 16:8) or short (LD 8:16) day lengths for 10 weeks. Animals in each photoperiod were further subdivided into groups housed in either mild (i.e., 20°C) or low (i.e., 8°C) temperatures. As shown with immunohistochemistry, voles that underwent gonadal regression in response to short photoperiods and long-day voles housed in low temperatures (and maintained large gonads) exhibit higher GnRH immunoreactive (GnRH-ir) neuron numbers in the preoptic area/anterior hypothalamus (POA/AH) relative to all other groups. In addition, voles that underwent gonadal regression in response to both short days and low temperatures did not exhibit an increase in GnRH-ir neuron numbers compared to long-day, mild-temperature controls. These data suggest that photoperiod and temperature interact to influence reproductive function potentially by alterations of the GnRH neuronal system.
Circadian Thermoregulation in Suckling Rabbit Pups
Authors: Jilge Burghart , Kuhnt Barbara , Landerer Waltraud
Published: May 2004

The rabbit pup is well suited to track the age-dependant development of periodic thermoregulation during the suckling period. Since the litters are regularly nursed once per day for a total of 3 to 4 min, an exogenous, metabolic, nonphotic periodic variable is supposed to have an impact on the 24-h rhythm of body temperature. The authors monitored the course of core body temperature during the suckling period of 20 pups by means of a transmitter implanted intraperitoneally on day 3 postpartum. The 24-h mean rose from an average of 37.8 ± 0.3 °C on day 4 of life to 39.5 ± 0.2 °C at weaning on day 27, for 2 out of 20 pups, and day 28, for 18 out of 20 pups. In constant dim illumination, the pups exhibited a 24-h rhythm even on postnatal day 4, which consolidated around days 5 to 7. The rhythm consisted of a significant anticipatory rise of 0.4 to 0.6 °C above the respective 24-h mean commencing 2.5 to 3.5 h prior to nursing. Milk intake was followed by a further increase of temperature for an additional 0.3 to 0.6 °C. Then the temperature dropped for 1.2 to 1.5 °C within 1 to 3 h and returned to average 3 to 5 h later. During a 48-h fast, the rhythm continued to exist, though in a modified shape: the anticipatory component persisted almost unchanged; a further elevation of temperature, however, did not occur. Thus, the anticipatory component apparently is generated endogenously and the second surge represents an exogenous suckling-induced, thermogenic peak. When maternal nursing was advanced for 15 min/day for a total of 5 h, the temperature rhythm of the pups followed the shift of the zeitgeber in parallel. These data confirm the assumption that a circadian rhythm exists during the first postnatal days of the rabbit and that this rhythm is entrained by the 24-h nursing rhythm. The authors suggest that the biological significance of a feeding entrainable oscillator (FEO) in the rabbit might be to activate the pups prior to the periodic nursing visit of the rabbit doe. Thus, the pups are prepared to quantitatively use the one and only short nursing episode per day for maximal milk ingestion.
Genetic and Environmental Influences on Short-Day Responsiveness in Siberian Hamsters (Phodopus sungorus)
Authors: Goldman Sharry L. , Dhandapani Krishnan , Goldman Bruce D.
Published: May 2004

Siberian hamsters are photoperiodic rodents that typically exhibit several physiological changes when exposed to a short-day photoperiod. However, development of the winter phenotype in short days is largely conditional on prior photoperiod history: Hamsters that have been reared in an exceptionally long day length (18 L) do not usually exhibit the winter phenotype after transfer to short days, whereas animals reared under “moderately” long days (16 L) are more variable in responsiveness to subsequent short-day exposure, with 20% to 30% generally failing to exhibit winter-type responses. Hamsters reared exclusively in an “intermediate” day length (14 L) are almost uniformly responsive to short photoperiod. In the present study, the authors examine the influence of photoperiod history on short-day responsiveness in a breeding line of hamsters that has been subjected to artificial selection for resistance to the effects of short days. The results demonstrate that photoperiod history is an important determinant of short-day responsiveness in both random-bred (UNS) hamsters and animals artificially selected and bred for nonresponsiveness to short photoperiod (PNR). The PNR hamsters have a reduced requirement for long-day exposure to evoke a state of unresponsiveness to short days. The results are discussed in relation to possible significance for the origin of population and species differences in photoperiod responsiveness.
The Human Circadian Pacemaker Can See by the Dawn's Early Light
Authors: Danilenko Konstantin V. , Wirz-Justice Anna , Kräuchi Kurt , Weber Jakob M. , Terman Michael
Published: May 2004

The authors' previous experiments have shown that dawn simulation at low light intensities can phase advance the circadian rhythm of melatonin in humans. The aim of this study was to compare the effect of repeated dawn signals on the phase position of circadian rhythms in healthy participants kept under controlled light conditions. Nine men participated in two 9-day laboratory sessions under an LD cycle 17.5:6.5 h, < 30:0 lux, receiving 6 consecutive daily dawn (average illuminance 155 lux) or control light (0.1 lux) signals from 0600 to 0730 h (crossover, random-order design). Two modified constant routine protocols before and after the light stimuli measured salivary melatonin (dim light melatonin onset DLMOn and offset DLMOff) and rectal temperature rhythms (midrange crossing time [MRCT]). Compared with initial values, participants significantly phase delayed after 6 days under control light conditions (at least –42 min DLMOn, –54 min DLMOff, –41 min MRCT) in spite of constant bedtimes. This delay was not observed with dawn signals (+10 min DLMOn, +2 min DLMOff, 0 min MRCT). Given that the endogenous circadian period of the human circadian pacemaker is slightly longer than 24 h, the findings suggest that a naturalistic dawn signal is sufficient to forestall this natural delay drift. Zeitgeber transduction and circadian system response are hypothesized to be tuned to the time-rate-of-change of naturalistic twilight signals.
Circadian Organization in Male Mice Lacking the Gene for Endothelial Nitric Oxide Synthase (eNOS–/–)
Authors: Kriegsfeld Lance J. , Drazen Deborah L. , Nelson Randy J.
Published: May 2004

Circadian (~24 h) rhythms in physiology and behavior are generated by the bilateral suprachiasmatic nucleus (SCN) of the anterior hypothalamus. For these endogenous rhythms to be synchronized with the external environment, light information must be transmitted to pacemaker cells within the SCN. This transmission of light information is accomplished via a direct retino-hypothalamic tract (RHT). Nitric oxide (NO), an endogenous gas that functions as a neurotransmitter, has been implicated as a messenger necessary for photic entrainment. Three isoforms of the enzyme that form nitric oxide, NO synthase, have been identified (a) in neurons (nNOS), (b) in the endothelial lining of blood vessels (eNOS), and (c) as an inducible form in macrophages (iNOS). The present study was undertaken to determine the specific role of eNOS in circadian organization and photic entrainment. Wild-type (WT) and eNOS–/– mice were initially entrained to a 14:10 light:dark (LD) cycle. After 3 weeks, the LD cycle was phase advanced. After an additional 3 weeks, animals were held in constant darkness (DD). eNOS–/– animals did not exhibit a deficit in the ability to entrain to the LD cycle, phase-shift locomotor activity, or free-run in constant conditions. Animals held in DD were killed after light exposure during either the subjective day or the subjective night to assess c-fos induction in the SCN. Light exposure during the subjective night increased c-fos protein expression in the SCN of both WT and eNOS–/– mice relative to animals killed after light exposure during the subjective day. Taken together, these findings suggest that endothelial isoform of NOS may not be necessary for photic entrainment in mice.
Day-Length Perception and the Photoperiodic Regulation of Flowering in Arabidopsis
Authors: Carr Isabelle A.
Published: May 2004

The flowering of Arabidopsis plants is accelerated by long-day photoperiods, and recent genetic studies have identified elements of the photoperiodic timing mechanism. These elements comprise genes that regulate the function of the circadian clock, photoreceptors, and downstream components of light signaling pathways. These results provide evidence for the role of the circadian clock in photoperiodic time measurement and suggest that photoperiod perception may follow Pittendrigh's external coincidence model. T-cycle experiments indicated that changes in the timing of circadian rhythms, relative to dawn and dusk, correlated with altered flowering time. Thus, the perception of photoperiod may be mediated by adjustments in the phase of the circadian cycle that arise upon re-entrainment to a different light-dark cycle. The nature of the rhythm underlying the floral response is not known, but candidate molecules have been identified.
Olfactory Cues Accelerate Reentrainment following Phase Shifts and Entrain Free-Running Rhythms in Female Octodon degus (Rodentia)
Authors: Governale Marcia M. , Lee Theresa M.
Published: May 2004

Social interactions between conspecifics is a type of nonphotic zeitgeber common to several species. In the diurnal rodent Octodon degus, social interactions enhance reentrainment after phase shifts and can act as a weak zeitgeber. Olfactory stimuli appear necessary for these effects since bulbectomy eliminates socially enhanced reentrainment. In Experiment 1, the authors examined whether stimulation of the main olfactory system was sufficient to enhance reentrainment after 6-h phase advances and delays in the adult female O. degus. When test animals received conspecific odor cues during reentrainment, they entrained 39% faster after phase advances ( p < 0.05) and 33% faster after phase delays ( p < 0.001) than when they did not receive odor cues. Thus, olfactory cues from distant female donors were sufficient to enhance rates of entrainment in female O. degus and provided results equivalent to earlier studies with donors and shifters housed in the cages together. In Experiment 2, the authors examined whether discrete 3-h and 1-h daily pulses of airborne odors from a group of 5 entrained female degus would be sufficient to produce entrainment of wheel-running activity in adult female conspecifics. During the period of exposure to 3-h pulses, 50% (4/8) of the subjects temporarily entrained to a 24-h cycle, while 12.5% (1/8) of the subjects fully entrained. Exposure to 1-h pulses allowed 37.5% (3/8) of the subjects to temporarily entrain and 12.5% (1/8) of the subjects to fully entrain. Duration of entrained episodes was positively correlated with [.Psi], daily onset of activity with respect to the timing of odor exposure (Pearson r = 0.731; p < 0.05), such that animals with the entraining odor pulse beginning during subjective day ([.Psi] = 7.8 h, CT 7.8 ± 1.4) had longer periods of entrainment (22.2 ± 5.6 days) than animals with the entraining pulse occurring during subjective night ([.Psi] =–4.6h; CT 19.4 ± 0.9; 5.6 ± 0.9 days; p < 0.001). In addition, for each animal, the combined duration of all episodes of 24-h entrainment correlated with increased period length ([.tau]) of free-running rhythms (Pearson r = 0.733; p < 0.05). Thus, daily discrete pulses of odors with durations of either 1 or3hfrom female conspecifics were sufficient to produce both temporary and full entrainment to a 24-h cycle in the majority of female O. degus, and the likelihood of long periods of entrainment correlated with long taus and coordination of the odor pulse with mid subjective day.
Distinct Pharmacological Mechanisms Leading to c-fos Gene Expression in the Fetal Suprachiasmatic Nucleus
Authors: Shearman Lauren P. , Weaver David R.
Published: May 2004

Maternal treatment with cocaine or a D1-dopamine receptor agonist induces c- fos gene expression in the fetal suprachiasmatic nuclei (SCN). Other treatments that induce c- fos expression in the fetal SCN include caffeine and nicotine. In the current article, the authors assessed whether these different pharmacological treatments activate c- fos expression by a common neurochemical mechanism. The results indicate the presence of at least two distinct pharmacological pathways to c- fos expression in the fetal rat SCN. Previous studies demonstrate that prenatal activation of dopamine receptors affects the developing circadian system. The present work shows that stimulant drugs influence the fetal brain through multiple transmitter systems and further suggests that there may be multiple pathways leading to entrainment of the fetal biological clock.
Temporal Reorganization of the Suprachiasmatic Nuclei in Hamsters with Split Circadian Rhythms
Authors: Gorman Michael R. , Yellon Steven M. , Lee Theresa M.
Published: May 2004

A dual oscillator basis for mammalian circadian rhythms is suggested by the splitting of activity rhythms into two components in constant light and by the photoperiodic control of pineal melatonin secretion and phase-resetting effects of light. Because splitting and photoperiodism depend on incompatible environmental conditions, however, these literatures have remained distinct. The refinement of a procedure for splitting hamster rhythms in a 24-h light-dark:light-dark cycle has enabled the authors to assess the ability of each of two circadian oscillators to initiate melatonin secretion and to respond to light pulses with behavioral phase shifting and induction of Fos-immunoreactivity in the suprachiasmatic nuclei (SCN). Hamsters exposed to a regimen of afternoon novel wheel running (NWR) split their circadian rhythms into two distinct components, dividing their activity between the latter half of the night and the afternoon dark period previously associated with NWR. Plasma melatonin concentrations were elevated during both activity bouts of split hamsters but were not elevated during the afternoon period in unsplit controls. Light pulses delivered during either the nighttime or afternoon activity bout caused that activity component to phase-delay on subsequent days and induced robust expression of Fos-immunoreactivity in the SCN. Light pulses during intervening periods of locomotor inactivity were ineffective. The authors propose that NWR splits the circadian pacemaker into two distinct oscillatory components separated by approximately 180 degrees, with each expressing a short subjective night.
Light-Induced Resetting of the Circadian Pacemaker: Quantitative Analysis of Transient versus Steady-State Phase Shifts
Authors: Watanabe Kazuto , Deboer Tom , Meijer Johanna H.
Published: May 2004

The suprachiasmatic nuclei of the hypothalamus contain the major circadian pacemaker in mammals, driving circadian rhythms in behavioral and physiological functions. This circadian pacemaker's responsiveness to light allows synchronization to the light-dark cycle. Phase shifting by light often involves several transient cycles in which the behavioral activity rhythm gradually shifts to its steady-state position. In this article, the authors investigate in Syrian hamsters whether a phase-advancing light pulse results in immediate shifts of the PRC at the next circadian cycle. In a first series of experiments, the authors aimed a light pulse at CT 19 to induce a phase advance. It appeared that the steady-state phase advances were highly correlated with activity onset in the first and second transient cycle. This enabled them to make a reliable estimate of the steady-state phase shift induced by a phase-advancing light pulse on the basis of activity onset in the first transient cycle. In the next series of experiments, they presented a light pulse at CT 19, which was followed by a second light pulse aimed at the delay zone of the PRC on the next circadian cycle. The immediate and steady-state phase delays induced by the second light pulse were compared with data from a third experiment in which animals received a phase-delaying light pulse only. The authors observed that the waveform of the phase-delay part of the PRC (CT 12-16) obtained in Experiment 2 was virtually identical to the phase-delay part of the PRC for a single light pulse (obtained in Experiment 3). This finding allowed for a quantitative assessment of the data. The analysis indicates that the delay part of the PRC—between CT 12 and CT 16—is rapidly reset following a light pulse at CT 19. These findings complement earlier findings in the hamster showing that after a light pulse at CT 19, the phase-advancing part of the PRC is immediately shifted. Together, the data indicate that the basis for phase advancing involves rapid resetting of both advance and delay components of the PRC.
Circadian Organization in a Diurnal Rodent, Arvicanthis ansorgei Thomas 1910: Chronotypes, Responses to Constant Lighting Conditions, and Photoperiodic Changes
Authors: Challet Etienne , Pitrosky Bruno , Sicard Bruno , Malan André , Pévet Paul
Published: May 2004

Little information is available on circadian organization in diurnal mammals. In the present study, the daily patterns of wheel-running activity were described in a diurnal rodent, Arvicanthis ansorgei Thomas 1910, as assessed by karyological analysis. Among 108 animals born in the colony and studied under a 12:12 light-dark cycle (lights on at 7:00 a.m.), the authors determined the timing of daily activity (i.e., mean onsets and offsets of pattern of locomotor activity) and the level of wheel-running activity performed during daytime versus nighttime. The activity pattern was essentially diurnal in 84% of individuals, 46% being active only during the light period ± 1 h (activity onsets and offsets at 6:20 a.m. and 7:40 p.m., respectively) and 38% being diurnal with a period of nocturnal activity longer than 1 h (activity onsets and offsets at 5:40 a.m. and 9:30 p.m., respectively). Of the 108 animals, 16% expressed a nocturnal activity with diurnal overlaps no longer than 1 h. In 6 diurnal individuals first exposed to constant light and then to constant dim red light, the endogenous period was shortened from 24.6 ± 0.1 to 24.0 ± 0.1 h, respectively. The numbers of wheel revolutions per day and during the active period remained unchanged between the two lighting conditions. In response to different photoperiodic changes from 16:08 to 08:16 light-dark cycles, the phase angle of photic synchronization, estimated by the daily onset of wheel-running activity in 6 diurnal animals, showed marked changes, its timing occurring 2 h before and 0.5 h after the onset of light under short and long photoperiods, respectively. The numbers of wheel revolutions per 24 h and during the active period were modified similarly according to photoperiodic changes. Finally, in 5 diurnal animals exposed to a 12:12 light-dark cycle, the daily pattern of general locomotor activity, determined by telemetry, was not modified by wheel availability. The data indicate that A. ansorgei is an interesting experimental model to understand the regulation of the circadian timing system in day-active species.
A Model for “Splitting” of Running-Wheel Activity in Hamsters
Authors: Oda Gisele A. , Friesen W. Otto
Published: May 2004

Splitting of locomotor activity rhythm in hamsters occurs when the animals are exposed for several weeks to constant light. The authors propose a mathematical model that explains splitting in terms of a switch in the sign of coupling of two oscillators, from positive to negative, due to long-term exposure to constant light. The model assumes that the two oscillators are not identical and that the negative coupling strengths achieved by each individual animal are variable. With these assumptions, the model provides a unified picture of all different splitting patterns presented by the hamsters, provides an explanation for why the two activity components cross each other during many patterns, and explains why the phase difference achieved by the split components is often near 180°.
Stress-Induced Hyperthermia Depends on Both Time of Day and Light Condition
Authors: Peloso Elizabeth , Wachulec Maciej , Satinoff Evelyn
Published: May 2004

Rats placed in an environment other than their home cage increase their body temperature (Tb) by more than 1 °C. This stress-induced hyperthermia is considered to be a fever, in the sense that the Tb rise seems to reflect an upward shift in the level of regulated Tb (set point). The circadian rhythm of Tb also reflects changes in set point. One might therefore expect to see differences in response to such stress during various phases of the light-dark (LD) cycle as Tb fluctuates between L and D. To test this, 3- to 6-month-old male Long-Evans rats were taken from their home cages (12:12 LD) and placed individually in a Plexiglas container for 30 min. Tb and activity were measured via telemetry. In the first experiment, rats were placed in the container during day (from 1 to 3 h after lights on) and night (from 1 to 3 h after lights off), with light on or off during the test. There was a significant Tb rise in response to placement in the container at all times except when the rats were tested during the night with light on in the container; in that condition there was no Tb rise. In the second experiment, the authors determined that 30 min of light in the home cage before the test did not affect Tb: If the light was on in the test situation, hyperthermia was inhibited, and if it was off, hyperthermia was as high as control levels. In the third experiment, to determine whether this effect was time dependent, the test was performed at 4-h intervals, with light on or off during the test. The strongest inhibiting effect of light was in early night. In the fourth experiment, the authors turned the lights on during early night while the rats were in their home cages. This reduced their Tb significantly by less than 0.3 °C. The authors conclude that both clock time and light condition during testing are factors affecting the Tb rise in response to stress.
Mapping of Elements Involved in Regulating Normal Temporal period and timeless RNA Expression Patterns in Drosophila melanogaster
Authors: Stanewsky Ralf , Lynch Kathlea S. , Brandes Christian , Hall Jeffrey C.
Published: May 2004

Although transcriptional regulation is a major force in generating circadian oscillations of clock molecules, posttranscriptional mechanisms also contribute to molecular rhythms. Applying novel transgenic period-luciferase constructs in transgenic Drosophila, the authors show that sequences within per's 5[.minute]-untranslated region mediate posttranscriptional regulation at the RNA level. Further mapping suggests that the relevant sequences for the correct phasing of period mRNAexpression are located within the first intron. The results are consistent with a clock-regulated temporal stabilization of period mRNA during its daily upswing in the morning. This process is inferred to depend on a function of the PERIOD and TIMELESS proteins, and could further contribute to the observed delay between RNA and protein accumulation. Similarly, applying timeless-luciferase constructs led to the demonstration that regulatory elements for proper temporal timeless expression are present ina4kbpromoter fragment and in sequences within the first intron. The results establish that, for normal rhythmicity, expression of clock genes requires regulation at the transcriptional, posttranscriptional, and posttranslational levels.
Photoperiodic Regulation of Circulating Leukocytes in Juvenile Siberian Hamsters: Mediation by Melatonin and Testosterone
Authors: Prendergast Brian J. , Hotchkiss Andrew K. , Nelson Randy J.
Published: May 2004

The reproductive system of Siberian hamsters ( Phodopus sungorus) undergoes rapid phenotypic responses to changes in day length that occur around the time of weaning. The present experiments tested whether the immune system of Siberian hamsters is similarly photoperiodicearly in life and whether photoperiodic changes in melatonin or gonadal hormone secretions mediate any such responses to day length. Circulating blood leukocyte concentrations (WBC) were measured in juvenile male Siberian hamsters that were gestated in long-days (LD), transferred to short-days (SD) on the day of birth, and subsequently either remained in SD or were transferred fromSD toLDat 18 days of age (day 18). WBC values were comparable between LD and SD hamsters on day 18. Between day 18 and day 32, SD hamsters exhibited a 3-fold increase in WBC, whereas LD hamsters failed to undergo a significant increase inWBCduring this interval. WBC of LD hamsters was significantly lower than that of SD hamsters on day 25 and on day 32. In LD housed males, peripheral injections of melatonin delivered so as to extend the nocturnal duration of elevated endogenous melatonin secretion (i.e., provided in late afternoon) on days 18-31 increasedWBCas measured on day 32. Peripubertal (day 17) gonadectomy abolished the immunosuppressive effect of LD exposure on WBC, and treatment with silasticimplants containing testosterone suppressed WBC independent of photoperiod treatment. These data indicate that juvenile Siberian hamsters are immunologically responsive to photoperiod and that the leukocyte responses to day length are the result of melatonin-mediated effects of photoperiod on testicular hormone secretion.
Noncircadian Regulation and Function of Clock Genes Period and Timeless in Oogenesis of Drosophila Melanogaster
Authors: Beaver L. M. , Rush B. L. , Gvakharia B. O. , Giebultowicz J. M.
Published: May 2004

Circadian clock genes are ubiquitously expressed in the nervous system and peripheral tissues of complex animals. While clock genes in the brain are essential for behavioral rhythms, the physiological roles of these genes in the periphery are not well understood. Constitutive expression of the clock gene period was reported in the ovaries of Drosophila melanogaster; however, its molecular interactions and functional significance remained unknown. This study demonstrates that period( per) and timeless( tim) are involved in a novel noncircadian function in the ovary. PER and TIM are constantly expressed in the follicle cells enveloping young oocytes. Genetic evidence suggests that PER and TIM interact in these cells, yet they do not translocate to the nucleus. The levels of TIM and PER in the ovary are affected neither by light nor by the lack of clock-positive elements Clock( Clk) and cycle( cyc). Taken together, these data suggest that per and tim are regulated differently in follicle cells than in clock cells. Experimental evidence suggests that a novel fitness-related phenotype may be linked to noncircadian expression of clock genes in the ovaries. Mated females lacking either per or tim show nearly a 50% decline in progeny, and virgin females show a similar decline in the production of mature oocytes. Disruption of circadian mechanism by either the depletion of TIM via constant light treatment or continuous expression of PER via GAL4/UAS expression system has no adverse effect on the production of mature oocytes.
Entrainment of 2 Subjective Nights by Daily Light:Dark:Light:Dark Cycles in 3 Rodent Species
Authors: Gorman Michael R. , Elliott Jeffrey A.
Published: May 2004

Recent work with exotic 24-h light:dark:light:dark (LDLD) cycles indicates surprising flexibility in the entrainment patterns of Syrian hamsters. Following exposure to an LDLD cycle, hamsters may adopt a form of rhythm splitting in which markers of subjective night (e.g., activity, melatonin) are expressed in each of the twice daily scotophases. This pattern contrasts markedly with that of conventionally entrained hamsters in which markers of subjective night are expressed once daily in only 1 of the 2 dark periods. The “split” entrainment pattern was examined further here in Syrian and Siberian hamsters and in mice exposed to LDLD 7:5:7:5, a condition that reliably induces split activity rhythms in all 3 species. The phase angle of entrainment and activity duration were generally similar comparing the 2 daily activity bouts in each species. The stability of this split entrainment state was assessed by deletions of photophases on individual days, by exposure to skeleton photoperiods, and by transfer to constant darkness. As in Syrian hamsters, the one-time substitution of darkness for one 7-h photophase did not grossly alter activity patterns of Siberian hamsters but acutely disrupted the split rhythms of mice. Skeleton light pulses of progressively shorter duration did not significantly alter split entrainment patterns of either Syrian or Siberian hamsters. Both species continued to exhibit stable entrainment with activity expressed in alternate scotophases of an LD 1:5 cycle presented 4 times daily. In contrast, the split activity rhythms of mice were not maintained under skeleton pulses. In constant darkness, rhythms of Siberian hamsters remained distinctly split for a minimum of 2 cycles. Split entrainment to these novel LDLD and 4-pulse skeleton lighting regimes demonstrates a marked degree of plasticity common to the circadian systems of several rodent species and identifies novel entrainment patterns that may be reliably elicited with simple environmental manipulations. Inter- and intraspecific differences in the stability of split activity rhythms likely reflect differences in coupling interactions between the component circadian oscillators, which, adopting separate phase relations to these novel LD cycles, yield a split entrainment pattern.
Nocturnal Activity in a Diurnal Rodent (Arvicanthis Niloticus): The Importance of Masking
Authors: Redlin Uwe , Mrosovsky N.
Published: May 2004

It is known that day-active Nile grass rats, Arvicanthis niloticus, increase the amount of activity in the night relative to that in the day when provided with running wheels. This was confirmed in the present study. Animals without a wheel displayed 69.0% of their general activity in the L phase of a 12:12 h light-dark cycle; animals provided with wheels had only 48.6% of their wheel revolutions in the light. The contribution of direct (masking) responses to light to the increased nocturnality of animals with wheels was examined in two experiments. In experiment 1, masking was tested by exposing the animals to repeated cycles of 30 min of entraining light and 30 min of a different, usually dimmer light, during the L phase of a 12:12 h light-dark cycle. For animals with wheels, there was more running during the 30-min pulses of dim light or darkness than during the 30-min periods of entraining light. In contrast, for animals without wheels, there was more general activity during the 30-min periods of entraining light than during the 30-min pulses of dim light or darkness. In experiment 2, the animals were first exposed to a 12:12 h light-dark cycle and then put on a 1:10:1:12 h LDLD skeleton photoperiod. Animals with wheels increased their running during the subjective day of the skeleton photoperiod compared to that in the actual day of the 12:12 h light-dark cycle. Animals without wheels showed similar levels of general activity during the subjective day of the skeleton photoperiod and the actual day of the 12:12 h cycle. These experiments demonstrate that when Nile rats have running wheels, their increased nocturnal activity is associated with an increased suppression of locomotion in direct response to light. It is possible that changes in masking responses to light may be an essential and integral component of switching between diurnal and nocturnal activity profiles.
Dec1 and Dec2 Expression is Disrupted in the Suprachiasmatic Nuclei of Clock Mutant Mice
Authors: Butler Matthew Preston , Honma Sato , Fukumoto Tatsuya , Kawamoto Takeshi , Fujimoto Katsumi , Noshiro Mitsuhide , Kato Yukio , Honma Ken-Ichi
Published: May 2004

DEC1 and DEC2 are basic helix-loop-helix transcription factors that functionally resemble negative feedback components of the mammalian circadian clock. The genes Dec1 and Dec2 are expressed rhythmically in the rat suprachiasmatic nuclei, and Dec1 expression is stimulated by light in a timedependent manner with the kinetics of an immediate early gene. DEC1 and DEC2 can inhibit CLOCK:BMAL1 transactivation of the clock gene Per1, suggesting that these transcription factors may help regulate circadian timing. The authors present data on the expression pattern of Dec1 and Dec2 in wild-type and homozygous Clock mutant mice. In the suprachiasmatic nuclei, the Clock mutation significantly reduces the expression of Dec1 and Dec2. Dec1 becomes arrhythmic; Dec2 remains weakly rhythmic in a 12L:12D light-dark cycle but is arrhythmic in constant darkness. A robust attenuation of the Dec1 and Dec2 signals in Clock mutant mice was detected in all brain areas examined. These data point to up-regulation of Dec1 and Dec2 by Clock in vivo.
Circadian Organization of a Subarctic Rodent, the Northern Red-Backed Vole (Clethrionomys Rutilus)
Authors: Tavernier Ronald J. , Largen Angela L. , Bult-Ito Abel
Published: May 2004

Arctic and subarctic environments are exposed to extreme light: dark (LD) regimes, including periods of constant light (LL) and constant dark (DD) and large daily changes in day length, but very little is known about circadian rhythms of mammals at high latitudes. The authors investigated the circadian rhythms of a subarctic population of northern red-backed voles ( Clethrionomys rutilus). Both wild-caught and third-generation laboratory-bred animals showed predominantly nocturnal patterns of wheel running when exposed to a 16:8 LD cycle. In LL and DD conditions, animals displayed large phenotypic variation in circadian rhythms. Compared to wheel-running rhythms under a 16:8 LD cycle, the robustness of circadian activity rhythms decreased among all animals tested in LLand DD (i.e., decreased chi-squared periodogram waveform amplitude). A large segment of the population became noncircadian (60% in DD, 72% in LL) within 8 weeks of exposure to constant lighting conditions, of which the majority became ultradian, with a few individuals becoming arrhythmic, indicating highly labile circadian organization. Wild-caught and laboratory-bred animals that remained circadian in wheel running displayed free-running periods between 23.3 and 24.8 h. A phase-response curve to light pulses in DD showed significant phase delays at circadian times 12 and 15, indicating the capacity to entrain to rapidly changing day lengths at high latitudes. Whether this phenotypic variation in circadian organization, with circadian, ultradian, and arrhythmic wheel-running activity patterns in constant lighting conditions, is a novel adaptation to life in the arctic remains to be elucidated.
Restricted Daytime Feeding Modifies Suprachiasmatic Nucleus Vasopressin Release in Rats
Authors: Kalsbeek Andries , van Heerikhuize Joop J. , Wortel Joke , Buijs Ruud M.
Published: May 2004

The authors have shown previously that vasopressin (VP) release from suprachiasmatic nucleus (SCN) efferents in rats is important for the timing of the circadian activity of the hypothalamo-pituitary-adrenal (HPA) axis, resulting in a circadian rise in corticosterone at dusk. When meals are supplied at a fixed time during the light period, however, this normal circadian activity of the HPA axis is strongly modified. Under such a restricted feeding regimen, a corticosterone peak appears just before the daily meal in addition to the circadian corticosterone peak at dusk. This feeding-associated rise in corticosterone is regarded as an SCN-independent circadian rhythm because it is sustained in SCN-lesioned animals. Despite these previous results, the authors investigated a putative involvement of SCN-derived VP in the control of the prefeeding corticosterone peak by measuring the intranuclear release of VP in the SCN and plasma corticosterone levels in rats in ad libitum feeding conditions as well as in animals that were obliged to feed during a 2-h period in the middle of the light period. Restricted daytime feeding caused clear changes in the daily release pattern of VP from SCN terminals. Both a delayed onset of the diurnal rise and a premature decline of the elevated daytime levels were observed, but the acrophase of the VP rhythm was not phase shifted. Concerning the circadian corticosterone peak, no phase shift of its acrophase was observed either. It is concluded that (1) restricted daytime feeding does affect SCN activity, (2) intranuclear release of VP within the SCN is an important mechanism to amplify and synchronize the circadian rhythms as dictated by the light/dark-entrained circadian pacemaker, and (3) VP release observed in animals on restricted feeding is completely compatible with the previously proposed inhibitory action of SCN-derived VP on the HPA axis.
Entrainment of Rat Circadian Rhythms by the Melatonin Agonist S-20098 Requires Intact Suprachiasmatic Nuclei But Not the Pineal
Authors: Redman Jennifer R. , Francis Andrew J. P.
Published: May 2004

S-20098 is a potent nonindolic melatonin agonist that has been shown to entrain free-running circadian rhythms. The current experiments examined the role of the suprachiasmatic nuclei (SCN) and of the pineal gland in the entrainment of circadian rhythms by S-20098. First, daily injections of S-20098 (1 and 10 mg/kg s.c.) were administered to SCN-and sham-lesioned rats. At both dose levels, circadian effects were noted in all sham-lesioned animals. Locomotor activity and body temperature rhythms in 3 of 5 sham-lesioned rats were entrained by the daily injections. In SCN-lesioned rats, S-20098 had no synchronizing or entraining effects at either dose level. These results show that S-20098 exerts its entraining effects on circadian rhythms via the circadian pacemaker located in the SCN. Second, the effects of daily injections of S-20098 (10 mg/kg s.c.) were examined in pinealectomized, sham-pinealectomized, and intact rats. All rats receiving S-20098, irrespective of surgical treatment, showed circadian changes. Rhythms in 81% of these animals entrained to daily administration of the compound, indicating that entrainment induced by S-20098 does not depend on an intact pineal. When injected with 10 mg/kg S-20098, 69% of rats, irrespective of surgical treatment, showed long-term modifications of free-running period that still were evident several weeks after administration ceased. If confirmed, this finding may have therapeutic implications in humans regarding the optimal mode and administration of S-20098 in a clinical setting.