bluuebunny/crossref_metadata_2025 huggingface dataset:
Unsymmetrical Bisacridines’ Interactions with ABC Transporters and Their Cellular Impact on Colon LS 174T and Prostate DU 145 Cancer Cells
Authors: Pawłowska Monika , Kulesza Jolanta , Paluszkiewicz Ewa , Augustin Ewa , Mazerska Zofia
Published: November 2024

Multidrug resistance (MDR) is a process that constitutes a significant obstacle to effective anticancer therapy. Here, we examined whether unsymmetrical bisacridines (UAs) are substrates for ABC transporters and can influence their expression in human colon LS 174T and prostate DU 145 cancer cells. Moreover, we investigated the cytotoxicity and the cellular response induced by UAs in these cells. The ATPase activities of MDR1, MRP1, and MRP2 were measured using vesicles prepared from insect Sf9 cells expressing particular ABC transporters. The gene expression and protein levels were analyzed using qPCR and Western blotting. The cellular effects were studied by MTT (cytotoxicity), flow cytometry (cell cycle analysis and phosphatidylserine externalization), and fluorescence microscopy. We showed that UAs are substrates for MDR1. Importantly, they did not influence remarkably the expressions of the ABCB1, ABCC1, and ABCC2 genes and the levels of the MDR1 and PXR proteins in the studied cells. Furthermore, the cytotoxicity and the level of apoptosis triggered by UAs in LS 174T cells possessing higher expressions of metabolic enzymes were lower compared with DU 145 cells. These results indicate that during possible UA treatment, the occurrence of drug resistance could be limited, which could favor the use of such compounds as potential candidates for future studies.
Efficient Degradation of Methylene Blue in Industrial Wastewater and High Cycling Stability of Nano ZnO
Authors: Liang Ping , Yang Weiye , Peng Hongyan , Zhao Shihua
Published: November 2024

Photocatalytic degradation research has attracted significant attention in the wake of the increasingly severe global challenge of water pollution. In this study, nano-ZnO was synthesized through a straightforward method using zinc acetate anhydrous as the precursor. The experimental results revealed that annealing conditions significantly influenced the bandgap energy (Eg) of ZnO, with a positive correlation observed between the intensity of photoluminescence (PL) spectra and photocatalytic activity. Under optimal annealing conditions at 300 °C for 1.5 h, the photocatalytic degradation efficiency of methylene blue (MB) exceeded 98% within 40 min of ultraviolet (UV) irradiation, surpassing the efficiencies reported for ZnO in recent studies. This high performance underscores the excellent photocatalytic activity of the synthesized ZnO samples. Furthermore, after five photocatalytic cycles, the degradation efficiency of MB remained above 90%, and the crystalline structure of the ZnO samples remained stable, demonstrating their exceptional structural stability during the photocatalytic process. Additionally, this study examined the effects of stirring conditions and different light sources on MB degradation, providing valuable insights for future research in related fields.
The Effect of Ultrasound and Lactic Acid Fermentation on the Selected Quality Parameters and Bioactive Compounds Content in Fermented Pumpkin (Cucurbita pepo L.)
Authors: Radziejewska-Kubzdela Elżbieta , Kidoń Marcin , Kowiel Angelika , Waszkowiak Katarzyna , Szymandera-Buszka Krystyna , Bednarek Marta , Kuligowski Maciej , Kobus-Cisowska Joanna , Mierzwa Dominik
Published: November 2024

Increasing the consumption of fruit and vegetables can be achieved by creating new products. A promising method seems to be the directed fermentation of vegetables. The aim of this study was to investigate the effect of ultrasonic pretreatment (US; 25 kHz; 5 min) and the lactic acid bacteria strain (LAB; Lactiplantibacillus plantarum 299v and Lacticaseibacillus rhamnosus GG) on the quality of fermented pumpkin (Cucurbita pepo L.). The pumpkin was inoculated with 5 log CFU/g of specific LAB strain. Fermentation was carried out for 7 days at 35 °C. Some samples were US treated at the washing stage. During fermentation, there was an increase in the LAB count of 3 logarithmic cycles compared to the initial inoculum. For L. rhamnosus, preceding fermentation by US treatment contributed to an increased bacteria count of 4 logarithmic cycles. In the case of fermentation with L. rhamnosus, the lactic acid content was significantly higher than for L. plantarum. These samples are also characterized by higher sensory properties, desirability of taste, and overall desirability. Fermentation contributed to a decrease in carotenoid and phenolic compounds content and an increase in the antioxidant capacity of the pumpkins, regardless of the bacterial strain.
Mechanical Behavior of Plasma-Treated Metal–Rubber Assemblies
Authors: Benyahia Lazhar , Ji Marisol , Poncin-Epaillard Fabienne
Published: November 2024

Metal–elastomer assemblies, such as aluminum–NBR and stainless steel–FKM, widely used for sealing or damping functions in various fields, are currently prepared with highly toxic bonding agents. To substitute the use of these liquids, plasma technologies were applied. The chemical nature of the plasma polymerized adhesives is found to have no influence on the viscoelastic properties of the elastomer. Furthermore, cohesive assemblies were prepared with acetylene, acrylic acid or maleic anhydride as plasma polymerized layers. Their adhesive performances were evaluated thanks to a tack-like test. Their adhesion mechanisms, even if complex, are namely identified as the interdiffusion of elastomer chains within the plasma-based polymer film and the thermodynamic adhesion. Specifically, we propose that the adhesiveness of metal–rubber assemblies, correlated to the maximum stress at failure in the tack-like test, is proportional to an energy per unit volume. This new variable is determined as the ratio of the surface tension to the thinness of the plasma adhesive.
Sorbus commixta Fruit Extract Suppresses Lipopolysaccharide-Induced Neuroinflammation in BV-2 Microglia Cells via the MAPK and NF-κB Signaling Pathways
Authors: Kim Yon-Suk , Jung Jin-Hwa , Kim Ki-Tae
Published: November 2024

Sorbus commixta Hedl. is a traditional medicinal plant in Korea, China, and Japan with known antioxidative, anti-inflammatory, anti-atherogenic, and anti-melanin activities. However, its anti-neuroinflammatory effects remain largely unknown. In this study, we investigated the inhibitory effects of S. commixta fruit extracts on lipopolysaccharide-stimulated pro-inflammatory factors in BV-2 microglia. We compared the anti-neuroinflammatory activity of S. commixta fruit water extract (SFW) and 70% ethanol extract using a nitric oxide assay. Our data indicated that the SFW (25–100 μg/mL) treatment significantly inhibited excessive nitric oxide production in lipopolysaccharide-stimulated BV-2 microglia compared to the 70% ethanol extract. It also attenuated the expression of inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor α. Moreover, SFW exhibited its anti-inflammatory properties by downregulating the expression of factors involved in the extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase pathways and by suppressing nuclear factor kappa B. Caffeic acid was identified as a primary component of SFW showing anti-neuroinflammatory activity. These findings suggest that SFW may offer substantial therapeutic potential for the treatment of neurodegenerative diseases involving microglia activation.
Noninvasive Assessments of Mitochondrial Capacity in People with Mitochondrial Myopathies
Authors: McCully Kevin K. , Bossie Hannah M. , Kendall Fran D.
Published: November 2024

People affected by mitochondrial myopathies (MITOs) are thought to have impaired skeletal muscle oxygenation. The aims of this study were to measure skeletal muscle mitochondrial capacity in MITO participants and able-bodied (AB) participants and evaluate the influence of muscle-specific endurance training in one MITO participant. Participants (n = 7) with mitochondrial disease and controls (n = 9) were tested (ages 18–54 years). Mitochondrial capacity (mVO2max) was measured using the rate constant of recovery of oxygen consumption (mVO2) after exercise in the forearm flexor muscles with near-infrared spectroscopy (NIRS). One MITO participant was tested before and after performing 18 forearm exercise sessions in 30 days. There were no differences between MITO and AB participants in mVO2max (MITO: 1.4 ± 0.1 min−1; AB: 1.5 ± 0.3 min−1; p = 0.29), resting mVO2 (MITO: −0.4 ± 0.2%/min; AB: −0.3 ± 0.1%/min; p = 0.23), or initial post exercise oxygen consumption rates (MITO: 4.3 ± 1.2%/min; AB: 4.4 ± 1.4%/min; p = 0.9). Exercise oxygen desaturation was greater in MITO (39.8 ± 9.7% range) than in AB (28 ± 8.8% range) participants, p = 0.02. The MITO participant who trained increased her mitochondrial capacity (58%) and muscle-specific endurance (24%) and had reduced symptoms of muscle fatigue. We found no evidence supporting in vivo impairment of forearm muscle mVO2max in genetically confirmed MITO participants. This is consistent with studies that report increased mitochondrial content, which offsets the decrease in mitochondrial function. Positive muscle adaptations to endurance training appear to be possible in people with MITOs. Characterization of study populations will be important when interpreting the relationship between in vivo mitochondrial capacity and mitochondrial disease.
Effect of Chemical Polishing on the Formation of TiO2 Nanotube Arrays Using Ti Mesh as a Raw Material
Authors: Li Wanshun , Zhang Shiqiu , Li Fei
Published: November 2024

As a unique form of TiO2, TiO2 nanotube arrays (TiO2NTAs) have been widely used. TiO2NTAs are usually prepared by Ti foil, with little research reporting its preparation by Ti mesh. In this paper, TiO2NTAs are prepared on a Ti mesh surface via an anodic oxidation method in the F-containing electrolyte. The optimal parameters for the synthesis of TiO2NTAs are as follows: the solvent is ethylene glycol and water; the electrolyte is NH4F (0.175 mol/L); the voltage is 20 V; and the anodic oxidation time is 40 min without chemical polishing. However, there is a strange phenomenon where the nanotube arrays grow only at the intersection of Ti wires, which may be caused by chemical polishing, and the other areas, where TiO2NTAs cannot be observed on the surface of Ti mesh, are covered by a dense TiO2 film. New impurities (the hydrate of TiO2 or other products) introduced by chemical polishing and attaching to the surface of the Ti mesh reduce the current of anodic oxidation and further inhibit the growth of TiO2 nanotubes. Hence, under laboratory conditions, for commercially well-preserved Ti mesh, there is no necessity for chemical polishing. The formation of TiO2NTAs includes growth and crystallization processes. For the growth process, F− ions corrode the dense TiO2 film on the surface of Ti mesh to form soluble complexes ([TiF6]2−), and the tiny pores remain on the surface of Ti mesh. Given the basic photoelectrochemical measurements, TiO2NTAs without chemical polishing have better properties.
Enhancing the Performance and Stability of Li-CO2 Batteries Through LAGTP Solid Electrolyte and MWCNT/Ru Cathode Integration
Authors: Na Dan , Yu Dohyeon , Kim Hwan , Yoon Baeksang , Lee David D. , Seo Inseok
Published: November 2024

Li-CO2 batteries (LCBs) have emerged as promising solutions for energy storage, with the added benefit of contributing to carbon neutrality by capturing and utilizing CO2 during operation. In this study, a high-performance LCB was developed using a Ge-doped LiAlGeTi (PO4)3 (LAGTP) solid electrolyte, which was synthesized via a solution-based method by doping Ge into NASICON-type LATP. The ionic conductivity of the LAGTP pellets was measured as 1.04 × 10−3 S/cm at 25 °C. The LCB utilizing LAGTP and an MWCNT/Ru cathode maintained a stable cycling performance over 200 cycles at a current density of 100 mA/g, with a cut-off capacity of 500 mAh/g. Post-cycle analysis confirmed the reversible electrochemical reactions at the cathode. The integration of LAGTP as a solid electrolyte effectively enhanced the ionic conductivity and improved the cycle life and performance of the LCB. This study highlights the potential of Ge-doped NASICON-type solid electrolytes for advanced energy-storage technologies and offers a pathway for developing sustainable and high-performance LCBs.
Effect of a Slow-Release Urea Nanofertilizer on Soil Microflora and Yield of Direct Seeded Rice (Oryza sativa L.)
Authors: Sehgal Yashika , Kalia Anu , Dhillon Buta Singh , Dheri Gurmeet Singh
Published: November 2024

Nitrogen fertilizers have a significant impact on the growth of rice. The overuse and inappropriate application of nitrogen fertilizers have resulted in environmental pollution, in addition to subjecting both humans and livestock to negative health hazards. Finding a viable substitute for traditional nitrogen fertilizers is crucial and essential to help improve crop yield and minimize environmental damage. Nano-nitrogen fertilizers offer a possible alternative to traditional fertilizers due to a slow/controlled release of nitrogen. The present work aimed to study the effect of a slow-release urea nanofertilizer on soil ammonical (NH4-N) and nitrate-N (NO3-N) content, culturable soil microflora, and soil enzyme activities in three different soil samples procured from Ludhiana and Patiala districts through a soil column study. Seven treatments, including 0, 50 (75 kg/ha N), 75 (112.5 kg/ha N), and 100% (150 kg/ha N) of the recommended dose (RD) of conventional urea and nano-urea fertilizer were applied. The leachate samples collected from nano-urea treatment exhibited NH4-N for the first two weeks, followed by NO3-N appearance. The higher NH4-N and NO3-N contents in the leachate were recorded for light-textured soil as compared to medium- and heavy-textured soil samples. The soil microbial counts and enzyme activities were recorded to be maximum in light-textured soils. Therefore, this slow-release formulation could be more useful for light-textured soils to decrease applied N-fertilizer losses, as well as for improving the soil microbial viable cell counts and soil enzyme activities. The effect of urea nanofertilizer on the growth and yield of direct-seeded rice (Oryza sativa L.) was also evaluated under field conditions. Both studies were performed independently. Numerically, the highest shoot height, fresh and dry shoot weight, and significantly maximum total chlorophyll, carotenoid, and anthocyanins were recorded in the T2 (100% RDF through nano-urea) treatment. The yield-attributing traits, including the number of filled grains and thousand-grain weight, were also recorded to have increased in T2 treatment. A numerical increase in NPK for plant and grain of rice at 100% RDN through nano-urea was recorded. The soil application of the product exhibited no negative effect on the soil microbial viable cell count on different doses of nano-urea fertilizer. The soil nitrogen fixer viable counts were rather improved in nano-urea treatments. The results reflect that nano-urea fertilizer could be considered as a possible alternative to conventional fertilizer.
Pasteurized Akkermansia muciniphila HB05 (HB05P) Improves Muscle Strength and Function: A 12-Week, Randomized, Double-Blind, Placebo-Controlled Clinical Trial
Authors: Kang Chang-Ho , Jung Eun-Soo , Jung Su-Jin , Han Yeon-Hee , Chae Soo-Wan , Jeong Do Yeun , Kim Byoung-Chan , Lee Seung-Ok , Yoon Sun-Jung
Published: November 2024

Background/Objectives: Sarcopenia, a condition marked by muscle wasting due to aging or inactivity, severely affects older populations. We previously showed that pasteurized Akkermansia muciniphila HB05 (HB05P), sourced from the breast milk of healthy Korean women, could mitigate muscle wasting in a dexamethasone-induced rat model. Here, we explored whether the oral administration of HB05P can enhance muscle strength and functionality in elderly individuals. Our objective was to determine if HB05P supplementation could benefit muscle performance in aging adults. Methods: We conducted a 12-week, double-blind, placebo-controlled clinical trial involving 100 individuals aged 60 and above, randomly assigned to receive either HB05P (1.0 × 1010 cells/day) or a placebo. Results: The HB05P group showed significant improvements in peak torque and peak torque per body weight of the left leg extensor muscles compared to the placebo group (p = 0.0103 and p = 0.0052). Furthermore, HB05P notably elevated follistatin levels, which counteract myostatin, relative to the placebo group (p = 0.0063). No notable safety concerns arose between the groups. Conclusions: HB05P is a promising postbiotic derived from Akkermansia muciniphila that may enhance muscle strength and be used as a safe postbiotic ingredient of Akkermansia muciniphila to improve muscle health.
Dietary Bovine Lactoferrin Reduces the Deleterious Effects of Lipopolysaccharide Injection on Mice Intestine
Authors: Blais Anne , Takakura Natsuko , Grauso Marta , Puel-Artero Caroline , Blachier François , Lan Annaïg
Published: November 2024

Background/Objectives: Injection of lipopolysaccharides (LPS) in experimental models induces a systemic inflammatory response that is associated with deleterious effects on intestinal morphology and physiology. In this study, we have studied in female mice the effects of dietary supplementation with bovine lactoferrin (bLF) given before intraperitoneal injection of LPS on jejunum and colon. Methods: The first study evaluated the efficiency of different bLF and LPS concentrations to determine the optimal experimental conditions. For the second study mice were fed with 1% bLF before the LPS challenge (3 mg/kg body weight). Plasmatic markers of inflammation, intestinal morphology, permeability, and expression of genes related to epithelial differentiation, epithelial barrier function and intestinal inflammation in both small intestine and colon were evaluated. Results: bLF ingestion before the LPS challenge reduced the TNF-α circulating concentration, compared to control animals. This decrease in plasma TNF-α was correlated with improved intestinal permeability. The morphology of jejunal epithelium, which was affected by LPS challenge, was partly maintained by bLF. Measurement of the expression of genes encoding proteins involved in epithelial differentiation, intestinal inflammation, and epithelial barrier function suggests an overall protective effect of bLF against the adverse effects of LPS in the jejunum. In the colon, the effects of bLF ingestion on the subsequent LPS challenge, although protective, remain different when compared with those observed on jejunum. Conclusions: Taken together, our data indicate that bLF dietary supplementation does have a protective effect on the deleterious intestinal alterations induced by LPS systemic inflammation.
Physical Activity Level and Dietary Intake Associated with Fat-Free Muscle Mass Changes During Intentional Weight Loss in Overweight and Obese Subjects
Authors: Gariballa Salah Eldin Elnagi , Al-Bluwi Ghada , Yasin Javed
Published: November 2024

Background: The prevalence of obesity and related complications is increasing relentlessly worldwide. The effect of intentional weight loss strategies for obese individuals on fat-free muscle mass (FFMM) and metabolic and general health is not well known. The aim of this research is to measure the effects of dietary intake and physical activity level on FFMM change during intentional weight loss in obese subjects. Materials and Methods: Nine hundred and sixty-five overweight and obese community free-living subjects had the effects of physical activity level and dietary intake on FFMM change during intentional weight loss assessed in a prospective longitudinal study. Anthropometric, physical activity, dietary intake, inflammatory markers, and oxidative damage were assessed at baseline and follow-up. Validated questionnaires were used to measure dietary intake and physical activity. We compared FFMM loss or gain between subjects stratified by calorie, protein, and fruit and vegetable intake and physical activity levels. The Cox proportional hazards analysis was used to determine the independent effects of dietary intake and physical activity on FFMM changes. Results: A total of 965 subjects [(mean (SD) age 39 ± 12 years, 801 (83%)] females] were assessed at baseline with follow-up for a period of 427 ± 223 days. Using the WHO criteria for body mass index (BMI), 284 (30%) subjects were found to be overweight and 584 (62%) were obese. We found significant correlations between fat–muscle mass ratio (FMR) and inflammatory and oxidative damage markers. After adjusting for important prognostic indicators, age, gender, occupation, physical activity, and fruit and vegetable consumption were found to be significantly associated with FFMM at baseline (p < 0.05). We found no statistically significant difference in dietary protein or amino acids intake in subjects who gained FFMM compared to those who lost FFMM both at baseline and follow-up. By contrast, high consumption of fruits and vegetable and increased calorie intake were associated with increased odds of FFMM gain (p < 0.05). Increased physical activity was independently associated with significant FFMM gain after adjusting for other important indicators ([hazard ratio (95% CI): 0.49 (0.25, 0.97); p = 0.039]. Conclusions: Increased physical activity and high calorie, fruit and vegetable intake are associated with FFMM preservation or gains during intentional weight loss in obese subjects.
Online Cognitive-Behavioral Therapy-Based Nutritional Intervention via Instagram for Overweight and Obesity
Authors: Rychescki Greta Gabriela , dos Santos Gabriela Rocha , Bertin Caroline Fedozzi , Pacheco Clara Nogueira , Antunes Luciana da Conceição , Stanford Fatima Cody , Boaventura Brunna
Published: November 2024

Background/Objectives: Obesity is a multifactorial chronic disease treated through lifestyle modifications, pharmacotherapy, and surgery. With the rise of social media, platforms like Instagram have become tools for lifestyle interventions. This study evaluated the impact of a cognitive-behavioral-therapy-based nutritional intervention via Instagram on body weight, eating behavior, and mental health in individuals with overweight and obesity. Methods: A 5-week online intervention delivered daily nutritional, cognitive, and behavioral content via a private Instagram account using live sessions, reels, feed posts, polls, and stories. Standardized dietary plans were sent by e-mail. Self-reported weight and waist circumference and questionnaires on eating behavior, self-esteem, stress, and anxiety were collected. Engagement and interaction were measured through comments, likes, number of followers, story retention, participation in live sessions, and direct messages. Results: The final sample included 66 participants (63 women), 27 with overweight and 39 with obesity, and a mean age of 40.5 ± 10.6 years. After the intervention, body weight decreased by 1.1 kg, while waist circumference remained unchanged. Participants with obesity showed significant improvements in binge eating, uncontrolled eating, self-esteem, stress, and anxiety, while those with overweight showed improvements in binge eating and stress. Weight loss was associated with reduced binge eating and lower cognitive restriction, while lower uncontrolled eating was related to decreased emotional eating, anxiety, and stress. Additionally, participation in live sessions was associated with reduced binge eating. Conclusions: This online intervention via Instagram was effective in improving weight loss, eating behavior, and mental health symptoms in participants with overweight and obesity.
Fish Oil Consumption: Its Effects on Bone and Blood Parameters of the Ovariectomized Rat Model of Osteopenia
Authors: Triantafyllou Aggeliki , Lelovas Pavlos P. , Galanos Antonis , Venetsanou Kyriaki , Passali Christina , Patsaki Anastasia , Pitidis Dimitrios , Kourkoulis Stavros K. , Dontas Ismene A.
Published: November 2024

Background/Objectives: The beneficial effects of ω-3 fatty acids on the cardiovascular system have been observed in many epidemiological studies; however, their effects on the skeleton and in particular on postmenopausal bone loss appear to vary. The present study’s purpose was to investigate the effects of oral fish oil (rich in ω-3 fatty acids) consumption on bone, plasma, and inflammation parameters in the ovariectomized (Ovx) rat model of osteopenia. Methods: Four Groups of ten rats each were separated into Non-Ovx receiving fish oil (2.8 g/kg body weight) (Non-Ovx + FO), Non-Ovx receiving isocaloric corn oil (Non-Ovx + CO), Ovx receiving fish oil (Ovx + FO), and Ovx receiving corn oil (Ovx + CO) daily for 4 months. Results: Tibial bone mineral density percentage changes from baseline were +4.09% in Non-Ovx + FO rats versus −2.99% in Non-Ovx + CO rats (p NS), and −5.73% in Ovx + FO rats versus −14.12% in Ovx + CO rats (p = 0.070), indicating a tendency to protect from bone loss. Fish oil exerted a beneficial effect on bone strength, as shown by significantly increased femoral fracture stress in Ovx + FO, compared to Ovx + CO rats (p = 0.05). The plasma’s total cholesterol was significantly reduced in both FO Groups versus the CO Groups (p < 0.001), while HDL-cholesterol decreased slightly in both FO Groups, significantly (p < 0.001) between Non-Ovx + FO versus Non-Ovx + CO. Interleukin 6 was reduced in both FO Groups, indicating the anti-inflammatory effect of fish oil consumption, which was highly significant (p < 0.001) between Non-Ovx + FO versus Non-Ovx + CO. Interleukin 10, TNF-α, and RANKL displayed non-significant changes. Conclusions: Among the skeletal and blood parameters studied, several, but not all, demonstrated a mild to significant beneficial effect of four-month fish oil consumption.
Propionate Production by Infant Fecal Microbiota Is Inversely Correlated with the Protein Glycation Level of Supplemented Infant Formula Ex Vivo
Authors: Bouillon Grégoire A. , Xie Zhuqing , Nielsen Dennis S. , Wiese Maria , Nauta Arjen
Published: November 2024

Background/Objectives: After birth, mothers provide the best nutrition for the healthy growth and development of their infants and the developing gut microbiota through breastfeeding. When breastfeeding is not or insufficiently available, infant formula is the only safe alternative. The production of infant formula includes heat-processing, which may induce protein glycation. Protein glycation has been shown to reduce protein digestion and absorption. The reduction in protein digestion and absorption because of protein glycation has been speculated to also impact gut comfort parameters as well as overnight sleep. Methods: As this could be partially due to the effect on the bacteria that reside in the infant’s gastrointestinal tract, we investigated whether protein glycation in infant formula impacts the composition and activity of infant gut microbiota by performing an in vitro study using the CoMiniGut colon model and fecal inocula obtained from a healthy six-month-old term infant. Incubations were performed for 24 h using a predigested infant formula-supplemented medium with varying levels of glycation (6.5–44.5%). Results: Our data indicate that high protein glycation increases microbial diversity and the relative abundance of Clostridium neonatale from 6.4% of the inoculum to around 25.5% of 20.8% glycation. Interestingly, propionate levels were inversely correlated with protein glycation levels after 24 h of incubation, with the 44.5% blocked lysine sample giving rise to 60% lower propionate levels as compared to the 6.4% sample. Higher propionate levels have been linked with longer uninterrupted sleep overnight, which could be indicative of the underlying mechanism of reduced crying/fussy time during nights for infants fed with a formula containing lower amounts of glycated protein.
Bondarzewia dickinsii Against Colitis-Associated Cancer Through the Suppression of the PI3K/AKT/COX-2 Pathway and Inhibition of PGE2 Production in Mice
Authors: Chen Junliang , Liu Shuai , Zhang Xin , Dai Xiaojing , Li Yu , Han Yonglin , Li Lanzhou
Published: November 2024

Background: Bondarzewia dickinsii (BD) is a newly discovered edible mushroom with rich nutritional components. This study presents a thorough analysis of the components of BD, examining its inhibitory effects and the underlying mechanisms by which BD influences colitis-associated cancer (CAC). Methods: AOM/DSS-induced CAC mice (male C57BL/6) were used, and a histopathological analysis, intestinal microbiota assessment, and metabolomics profiling were carried out, as well as an evaluation of relevant proteins and factors, to investigate the CAC-inhibitory effects of BD. Results: BD is rich in nutritional components, including a total sugar content of 37.29% and total protein content of 24.9%. BD significantly diminished colon inflammation, as well as the size and quantity of tumors. In addition, BD modified the diversity of intestinal microbiota and changed the levels of 19 serum metabolites, including arachidonic acid. BD significantly reduced prostaglandin E2 (PGE2) and cyclooxygenase-2 (COX-2) in colon tissue. Furthermore, it was found to inhibit the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/COX-2 signaling pathway. Conclusions: In general, BD inhibited the onset and progression of CAC by modulating the composition of intestinal microbiota and metabolite levels, suppressing the PI3K/AKT/COX-2 pathway, and decreasing PGE2 expression. This study provides a significant reference for the development of BD as a dietary supplement and pharmaceutical agent in the treatment of CAC.
Adherence to the Cancer Prevention Recommendations from World Cancer Research Fund/American Institute for Cancer Research After Cancer Diagnosis on Mortality in South Korea
Authors: Won Donghyun , Lee Jeeyoo , Cho Sooyoung , Baek Ji Yoon , Kang Daehee , Shin Aesun
Published: November 2024

Background/Objectives: The World Cancer Research Fund/American Institute for Cancer Research recommends following the recommendations for cancer prevention even after cancer diagnosis. To provide evidence on the potential benefits of adherence on improved survival, we investigated the effects of post-diagnostic adherence to the recommendations regarding diet, physical activity, and body weight on all-cause mortality among Korean cancer survivors. Methods: Among the total number of cancer survivors (n = 173,195) recruited from 2004 to 2013 for the Health Examinees study, 5485 were selected for the analyses and classified by tertiles according to the adherence score. The Cox proportional hazards model was used to estimate the adjusted hazard ratios (HR) and their 95% confidence intervals (CI) of the adherence groups on all-cause mortality. Results: Although no clear association was observed overall during a mean follow-up of 10.1 (standard deviation = 3.0) years, reduced mortality was observed for the middle (HR = 0.74 [95% CI = 0.51–1.08]) and highest adherent group (0.66 [0.43–0.99]) in comparison to the lowest adherent group among long-term survivors (>5 years after cancer diagnosis). Conclusions: Among the cancer prevention recommendation items, “be physically active” and “limit consumption of fast foods” were inversely associated with mortality. Adhering to the WCRF/AICR cancer prevention recommendations may help improve the prognosis of long-term cancer survivors.
Dose–Response Associations Between Diet and Risk of Rheumatoid Arthritis: A Meta-Analysis of Prospective Cohort Studies
Authors: Dong Yuanyuan , Greenwood Darren , Webster James , Uzokwe Chinwe , Tao Jinhui , Hardie Laura , Cade Janet
Published: November 2024

To provide a systematic and quantitative summary of dietary factors and rheumatoid arthritis (RA) risk. A systematic review and meta-analysis included prospective cohort studies from 2000 to 2024 reporting relative risks (RRs) with 95% confidence intervals (CIs) for RA incidence relating to 32 different dietary exposures. Linear and non-linear dose–response analyses were conducted. Thirty studies were included, involving 2,986,747 participants with 9,677 RA cases. Linear dose–response analysis suggested that each 2-unit per week increase in total alcohol intake was linked to 4% risk reduction (RR (95%-CI), heterogeneity (I2), NutriGrade score: 0.96 (0.94, 0.98), 58%, moderate certainty), and beer consumption was associated with a 10% reduction per 2 units/week increase (0.90 (0.84, 0.97), 0%, very low certainty). Each 2-unit/week increase in total alcohol intake was associated with a 3% decrease in seropositive RA risk (0.97 (0.96, 0.99), 28%, moderate certainty). Increased intakes of fruit (per 80 g/day) and cereals (per 30 g/day) were associated with 5% (0.95 (0.92, 0.99), 57%, moderate certainty) and 3% (0.97 (0.96, 0.99), 20%, moderate certainty) reduced risk, respectively. Conversely, tea consumption showed a 4% increased risk per additional cup/day (1.04 (1.02, 1.05), 0%, moderate certainty). Non-linear associations were observed for total coffee, vegetables, oily fish, and vitamin D supplementation. Data on dietary patterns and specific micronutrients were limited. The findings suggest that moderate alcohol consumption and a higher intake of fruits, oily fish, and cereals are associated with a reduced risk of RA, while tea and coffee may be linked to an increased risk. Optimising dietary intake of certain food components may reduce RA risk, despite moderate-quality evidence.
Agathisflavone Modulates Reactive Gliosis After Trauma and Increases the Neuroblast Population at the Subventricular Zone
Authors: Castro e Silva Juliana Helena , Pieropan Francesca , Rivera Andrea Domenico , Butt Arthur Morgan , Costa Silvia Lima
Published: November 2024

Background: Reactive astrogliosis and microgliosis are coordinated responses to CNS insults and are pathological hallmarks of traumatic brain injury (TBI). In these conditions, persistent reactive gliosis can impede tissue repopulation and limit neurogenesis. Thus, modulating this phenomenon has been increasingly recognized as potential therapeutic approach. Methods: In this study, we investigated the potential of the flavonoid agathisflavone to modulate astroglial and microglial injury responses and promote neurogenesis in the subventricular zone (SVZ) neurogenic niche. Agathisflavone, or the vehicle in controls, was administered directly into the lateral ventricles in postnatal day (P)8-10 mice by twice daily intracerebroventricular (ICV) injections for 3 days, and brains were examined at P11. Results: In the controls, ICV injection caused glial reactivity along the needle track, characterised immunohistochemically by increased astrocyte expression of glial fibrillary protein (GFAP) and the number of Iba-1+ microglia at the lesion site. Treatment with agathisflavone decreased GFAP expression, reduced both astrocyte reactivity and the number of Iba-1+ microglia at the core of the lesion site and the penumbra, and induced a 2-fold increase on the ratio of anti-inflammatory CD206+ to pro-inflammatory CD16/32+ microglia. Notably, agathisflavone increased the population of neuroblasts (GFAP+ type B cells) in all SVZ microdomains by up to double, without significantly increasing the number of neuronal progenitors (DCX+). Conclusions: Although future studies should investigate the underlying molecular mechanisms driving agathisflavone effects on microglial polarization and neurogenesis at different timepoints, these data indicate that agathisflavone could be a potential adjuvant treatment for TBI or central nervous system disorders that have reactive gliosis as a common feature.
Plant Taxa as Raw Material in Plant-Based Meat Analogues (PBMAs)—A Patent Survey
Authors: Kostrakiewicz-Gierałt Kinga
Published: November 2024

Background/Objectives: The environmental problems associated with meat production, the pain and distress of animals, and health problems have contributed to the increased appreciation of meat alternatives. Methods: The review of patented inventions presenting plant-based meat analogues (PBMAs) issued in the years 2014–2023 was conducted according to PRISMA statements across the ISI Web of Science, as well as Google Patents and Espacenet Patent Search engines. Results: The analysis of 183 patents showed an increase in patent numbers in the years 2020–2022, with the greatest number of patents developed by teams consisting of two authors. The authors and patent applicants were mainly affiliated with the United States, while Société des Produits Nestlé S.A. emerged as the leader among applicant institutions. The International Patent Classification (IPC) codes were given to 177 patents, which were mainly classified as Human Necessities (Section A). In total, inventors mentioned 184 taxa, including 28 genera, 1 section, 144 species, 5 subspecies and 6 varieties of vascular plants. The majority of taxa showed a high edibility rating and belonged to perennials, mainly herbaceous plants representing the families Fabaceae, Poaceae and Brassicaceae. The analysis of patents showed that plants are a promising source of protein, lipids, fibre, polyphenols, starch and gum in meat analogues. At the same time, the noticed slight use of numerous taxa, despite the substantial content of valuable constituents as well as high edibility rates, presumably might be caused by their underutilization in numerous regions of the world. Conclusions: The direction of future studies should focus on searching for novel plant-based meat analogues based on underutilized, promising plant sources and investigations of their usefulness.
Exploring Physical Activity, Sleep, and Nutrition’s Role in Fatigue Among Post-COVID-19 Patients
Authors: Pink Isabell , Wiestler Miriam , Pueschel Lea , Ruwisch Jannik , Drick Nora , Boblitz Lennart , Scharbau Michele , Welte Tobias , Haufe Sven , Tegtbur Uwe , Kück Momme , Kerling Arno , Beyer Sebastian
Published: November 2024

Background/Objectives: A significant number of patients experience prolonged symptoms following COVID-19 in particular cases of fatigue. Yet, pathomechanisms of COVID-19-related fatigue remain unclear. Methods: This study investigated patients after confirmed SARS-CoV-2 infection (PC) with relevant fatigue according to the Fatigue Assessment Scale (≥22 points) to rule out malnutrition as a driving factor for fatigue and to evaluate daily activity and sleep characteristics. Dietary behavior was recorded through food diaries and physical activity by self-reported (questionnaires) and objective (activity tracker) outcomes. Data were collected over a 7-day period and compared with a healthy control group (HC). A subgroup analysis of patients with fatigue and severe fatigue, as well as a sex-specific analysis, were included. Results: No significant differences in dietary intake were observed, but an indication toward a healthier Mediterranean diet in PC patients with a median Mediterranean Diet Score of 4 (IQR 3, 5) in HC vs. 5 (IQR 3, 6) in PC (p = 0.24). There were also no differences in physical activity, either by objective or subjective measures. However, the median sleep duration was 49 min longer in PC patients (p = 0.003). Conclusions: In conclusion, malnutrition did not significantly contribute to fatigue, yet patients with COVID-19-related fatigue showed increased sleep duration. As sleep characteristics play a crucial role in mental and physical wellbeing, the association of sleep, physical activity, and fatigue should be evaluated in further studies.
Back to Roots: Dysbiosis, Obesity, Metabolic Syndrome, Type 2 Diabetes Mellitus, and Obstructive Sleep Apnea—Is There an Objective Connection? A Narrative Review
Authors: Protasiewicz-Timofticiuc Diana Cristina , Bădescu Diana , Moța Maria , Ștefan Adela Gabriela , Mitrea Adina , Clenciu Diana , Efrem Ion Cristian , Roșu Maria Magdalena , Vladu Beatrice Elena , Gheonea Theodora Claudia , Moța Eugen , Vladu Ionela Mihaela
Published: November 2024

In recent decades, it has become clear that the gut is more than just a digestive organ; it also functions as an immune organ with regulatory capabilities and acts as a “second brain” that influences brain function due to the presence and regulatory roles of the gut microbiota (GM). The GM is a crucial component of its host and significantly impacts human health. Dysbiosis, or microbial imbalance, has been closely linked to various diseases, including gastrointestinal, neurological, psychiatric, and metabolic disorders. The aim of this narrative review is to highlight the roles of the GM in maintaining metabolic health. Sleep is a vital biological necessity, with living organisms having evolved an internal sleep–wake rhythm that aligns with a roughly 24 h light/dark cycle, and this is known as the circadian rhythm. This cycle is essential for tissue repair, restoration, and overall optimal body functioning. Sleep irregularities have become more prevalent in modern society, with fast-paced lifestyles often disrupting normal sleep patterns. Urban living factors, such as fast food consumption, shift work, exposure to artificial light and nighttime noise, medications, and social activities, can adversely affect circadian rhythms, with dysbiosis being one of the many factors incriminated in the etiology of sleep disorders.
The Release of Organic Acids and Low Molecular Weight Carbohydrates from Matcha Tea After In Vitro Digestion
Authors: Nekvapil Jiří , Sumczynski Daniela , Salek Richardos Nikolaos , Bučková Martina
Published: November 2024

Background/Objectives: This study tested the influence of in vitro digestion on the release of organic acids and low molecular weight saccharides of matcha. Methods: The concentrations of analytes in the raw and undigested portion of matcha were measured using HPLC with spectrometric and refractometric detection to establish their residual values after a two-step enzymatic digestion that was finally presented as a retention factor. Results: It was established that dry matter digestibility values after simulated gastric and both gastric and intestinal phases were 67.3 and 85.9%, respectively. Native matcha, citric acid (44.8 mg/g), malic acid (32.2 mg/g), trehalose (36.1 mg/g), and L-arabinose (8.20 mg/g) reached the highest values and were predominant, whereas D-fructose, xylose, maltose, and saccharose were not detected. Regarding gastric phase digestion, succinic and malic acids, trehalose and D-glucose were the worst-releasing compounds and their remaining factors reached 34, 19, 18, and 50%, respectively, whereas L-arabinose was completely released. Focusing on gastric and small intestinal digestion, the least-releasing compounds of matcha tea leaves were succinic acid and trehalose, with their retention factors at 7 and 13%, which can proceed with the leaf matrix to the large intestine. Conclusions: Malic, oxalic, and citric acids, the carbohydrates D-glucose, L-arabinose, and L-rhamnose, are almost entirely released from matcha tea during digestion in the stomach and small intestine and can be available for absorption in the small intestine. In the measurement of oxalic acid, considering that the process of shading tea leaves increases the concentration of this acid and its retention factor value is too small, it would be appropriate in the future to evaluate the recommended maximum daily intake of matcha tea for people sensitive to the formation of urinal stones.
Energy, Macronutrients and Micronutrients Intake Among Pregnant Women in Lebanon: Findings from the Updated Lebanese National Food Consumption Survey (LEBANON-FCS)
Authors: Mahfouz Rana , Akiki Marie-Therese , Ndayra Vanessa , El Khoury Rebecca , Chawi Marise , Hatem Majida , Hanna-Wakim Lara , Sacre Yonna , Hoteit Maha
Published: November 2024

Background: Pregnancy is a crucial period for maternal and fetal health, and in Lebanon, where cultural and economic factors influence dietary practices, there is an urgent need to evaluate the food consumption patterns and diet quality of pregnant women. Aim: To evaluate the food consumption patterns, energy intake, as well as macro- and micro-nutrient intake among a nationally representative sample of Lebanese pregnant women aged 18–49 years old. Methods: A cross-sectional study was carried out from March to October 2023, involving 500 pregnant women from all eight Lebanese governorates. Sociodemographic and medical information was gathered, food consumption was evaluated using a validated Food Frequency Questionnaire (FFQ) and three 24-h recall, and anthropometric measurements were recorded. Results: The current population did not meet the USDA healthy pattern recommendations for whole grain, seafood, dairy, nuts, seeds and soy products consumption but exceeded the guidelines for vegetables, meats, poultry, eggs, oils, and refined grains. According to Mediterranean diet guidelines, the sample fell short in recommended intakes for fruits, olives/nuts/seeds, eggs, and olive oil, while surpassing the recommended levels for potatoes, legumes, pulses, sweets, red meat, processed meat, and fish and seafood. None of the participants met the energy requirements for their trimester and age group. In terms of macronutrient intake, the requirements for protein, unsaturated fats, and fiber were not met, while intakes of fats and sugars were exceeded. Regarding micronutrients, the recommended levels were not fully achieved, with particularly low intakes of vitamin D and iodine, as well as inadequate adherence to recommendations for iron, calcium, vitamin A, vitamin E, zinc, and choline. Additionally, a third of the participants did not meet the recommended intakes for folate and vitamin B12. Conclusions: The findings reveal significant dietary inadequacies among the current population, with participants failing to meet essential recommendations for whole grains and key food groups, alongside insufficient energy intake for their trimesters and age groups. Critical micronutrient deficiencies, particularly in vitamin D, iodine, and B vitamins, highlight the urgent need for targeted nutritional interventions and public health initiatives to improve dietary practices among pregnant women in Lebanon.
Göttingen Minipigs as a Model for Assessing the Impact of Drugs on the Gut and Milk Microbiota—A Preliminary Study
Authors: Bencivenni Silvia , Brigidi Patrizia , Zannoni Augusta , Ventrella Domenico , Elmi Alberto , Bacci Maria Laura , Forni Monica , D’Amico Federica , Turroni Silvia
Published: November 2024

Background: Early gut microbiota (GM) dysbiosis can affect a child’s health and has been linked to the onset of pathologies later in life. Breast milk is recognized as a major driver of the structure and dynamics of an infant’s GM. In addition to nutritious and prebiotic compounds, milk contains a microbiota that is shaped by several maternal factors, including gut microorganisms and medications. However, the impact of the latter on the milk microbiota is still largely unknown. Here, we investigated the effects of amoxicillin on the milk microbiota and GM of lactating Göttingen Minipigs sows, a promising model for studying medication transfer during lactation. Methods: Three sows were given amoxicillin (7 mg/kg/day) for three weeks starting from the second week after farrowing. Fecal and milk samples were collected before and after treatment and profiled by 16S rRNA amplicon sequencing. Results: Göttingen Minipigs’ milk microbiota showed similarities to that of humans and conventional sows, with minor compositional shifts after treatment. At the genus level, we observed a decrease in Staphylococcus and o_Bacteroidales;Other;Other, and an increasing trend in the abundance of Streptococcus, Stenotrophomonas, f_Rhodobacteraceae;Other, Proteiniclasticum, f_Propionibacteriaceae;Other and Gemella. In contrast, as expected, the GM was strongly affected by amoxicillin, even at the phylum level. Conclusions: In addition to demonstrating the relevance of Göttingen Minipigs as a valid model for studying the impact of medications on maternal milk and GM, our findings suggest that the milk microbiota may be more stable during antibiotic treatment than the GM.