bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of conservation tillage on wheat performance and its microbiome
Authors: Romano Ida , Bodenhausen Natacha , Basch Gottlieb , Soares Miguel , Faist Hanna , Trognitz Friederike , Sessitsch Angela , Doubell Marcé , Declerck Stéphane , Symanczik Sarah
Published: August 2023

Winter wheat is an important cereal consumed worldwide. However, current management practices involving chemical fertilizers, irrigation, and intensive tillage may have negative impacts on the environment. Conservation agriculture is often presented as a sustainable alternative to maintain wheat production, favoring the beneficial microbiome. Here, we evaluated the impact of different water regimes (rainfed and irrigated), fertilization levels (half and full fertilization), and tillage practices (occasional tillage and no-tillage) on wheat performance, microbial activity, and rhizosphere- and root-associated microbial communities of four winter wheat genotypes (Antequera, Allez-y, Apache, and Cellule) grown in a field experiment. Wheat performance (i.e., yield, plant nitrogen concentrations, and total nitrogen uptake) was mainly affected by irrigation, fertilization, and genotype, whereas microbial activity (i.e., protease and alkaline phosphatase activities) was affected by irrigation. Amplicon sequencing data revealed that habitat (rhizosphere vs. root) was the main factor shaping microbial communities and confirmed that the selection of endophytic microbial communities takes place thanks to specific plant–microbiome interactions. Among the experimental factors applied, the interaction of irrigation and tillage influenced rhizosphere- and root-associated microbiomes. The findings presented in this work make it possible to link agricultural practices to microbial communities, paving the way for better monitoring of these microorganisms in the context of agroecosystem sustainability.
Genome-wide identification and molecular evolution of NAC gene family in Dendrobium nobile
Authors: Fu Chun , Liu MingYu
Published: August 2023

NAC transcription factors are an important genes that regulate plant growth and development, and can regulate functions such as fruit ripening in plants. Based on genome data of Dendrobium nobile, the NAC gene family was identified and analyzed by bioinformatics methods. In this study, we identified 85 NAC genes in Dendrobium nobile genome, and systematically analyzed the NAC gene family. We found that they were distributed unevenly in the nineteen chromosomes. The amino acid length of D. nobile NAC gene family (DnoNACs) ranged from 80 to 1065, molecular weight ranged from 22.17 to 119.02 kD, and isoelectric point ranged from 4.61~9.26. Its promoter region contains multiple stress responsive elements, including light responsive, gibberellin-responsive, abscisic acid responsiveness, MeJA-responsiveness and drought-inducibility elements. Phylogenetic analysis indicates that the D. nobile NAC gene family is most closely related to Dendrobium catenatum and Dendrobium chrysotoxum. Analysis of SSR loci indicates that the fraction of mononucleotide repeats was the largest, as was the frequency of A/T. Non-coding RNA analysis showed that these 85 NAC genes contain 397 miRNAs. The collinearity analysis shows that 9 collinear locis were found on the chromosomes of D. nobile with Arabidopsis thaliana, and 75 collinear locis with D.chrysotoxum. QRT-PCR experiment under different salt concentration and temperature conditions verified the response mechanism of DnoNAC gene family under stress conditions. Most DnoNAC genes are sensitive to salt stress and temperature stress. The results of this study provide a reference for further understanding the function of NAC gene in D. nobile.
Genome-wide survey and characterization of microsatellites in cashew and design of a web-based microsatellite database: CMDB
Authors: Savadi Siddanna , Muralidhara B. M. , Venkataravanappa V. , Adiga J. D.
Published: August 2023

The cashew is an edible tree nut crop having a wide range of food and industrial applications. Despite great economic importance, the genome-wide characterization of microsatellites [simple sequence repeats (SSRs)] in cashew is lacking. In this study, we carried out the first comprehensive genome-wide microsatellites/SSRs characterization in cashew and developed polymorphic markers and a web-based microsatellite database. A total of 54526 SSRs were discovered in the cashew genome, with a mean frequency of 153 SSRs/Mb. Among the mined genome-wide SSRs (2-6 bp size motifs), the dinucleotide repeat motifs were dominant (68.98%) followed by the trinucleotides (24.56%). The Class I type of SSRs (≥20 bp) were 45.10%, while Class II repeat motifs (≥12–<20 bp) were 54.89% of the total genomic SSRs discovered here. Further, the AT-rich SSRs occurred more frequently in the cashew genome (84%) compared to the GC-rich SSRs. The validation of the in silico-mined genome-wide SSRs by PCR screening in cashew genotypes resulted in the development of 59 polymorphic SSR markers, and the polymorphism information content (PIC) of the polymorphic SSR markers ranged from 0.19 to 0.84. Further, a web-based database, “Cashew Microsatellite Database (CMDB),” was constructed to provide access to the genome-wide SSRs mined in this study as well as transcriptome-based SSRs from our previous study to the research community through a user-friendly searchable interface. Besides, CMDB provides information on experimentally validated SSRs. CMDB permits the retrieval of SSR markers information with the customized search options. Altogether, the genome-wide SSRs characterization, the polymorphic markers and CMDB database developed in this study would serve as valuable marker resources for DNA fingerprinting, germplasm characterization, genetic studies, and molecular breeding in cashew and related Anacardium species.
Transcriptional control of hydrogen peroxide homeostasis regulates ground tissue patterning in the Arabidopsis root
Authors: Oh Jiyeong , Choi Ji Won , Jang Sejeong , Kim Seung Woo , Heo Jung-Ok , Yoon Eun Kyung , Kim Soo-Hwan , Lim Jun
Published: August 2023

In multicellular organisms, including higher plants, asymmetric cell divisions (ACDs) play a crucial role in generating distinct cell types. The Arabidopsis root ground tissue initially has two layers: endodermis (inside) and cortex (outside). In the mature root, the endodermis undergoes additional ACDs to produce the endodermis itself and the middle cortex (MC), located between the endodermis and the pre-existing cortex. In the Arabidopsis root, gibberellic acid (GA) deficiency and hydrogen peroxide (H2O2) precociously induced more frequent ACDs in the endodermis for MC formation. Thus, these findings suggest that GA and H2O2 play roles in regulating the timing and extent of MC formation. However, details of the molecular interaction between GA signaling and H2O2 homeostasis remain elusive. In this study, we identified the PEROXIDASE 34 (PRX34) gene, which encodes a class III peroxidase, as a molecular link to elucidate the interconnected regulatory network involved in H2O2- and GA-mediated MC formation. Under normal conditions, prx34 showed a reduced frequency of MC formation, whereas the occurrence of MC in prx34 was restored to nearly WT levels in the presence of H2O2. Our results suggest that PRX34 plays a role in H2O2-mediated MC production. Furthermore, we provide evidence that SCARECROW-LIKE 3 (SCL3) regulates H2O2 homeostasis by controlling transcription of PRX34 during root ground tissue maturation. Taken together, our findings provide new insights into how H2O2 homeostasis is achieved by SCL3 to ensure correct radial tissue patterning in the Arabidopsis root.
What affects you? A conversation analysis of exploring emotions during reflection sessions in Dutch general practitioner training
Authors: van Braak Marije , Schaepkens Sven P. C. , van Dolder Elise , Dral Luna K. , van der Horst Zoey , Houben Daan B. , Mees Emma E.
Published: August 2023

Introduction

In Dutch training for general practitioners (GPs), reflection on professional practice is key to their training. Such reflection is considered beneficial for professional development, especially when it entails discussing the emotional dimension of practice experiences. In the GP context, invitations to share the emotional side of things, such as “how did that make you feel?” are considered functional; yet, they are also sometimes viewed by participants as ‘grilling’, ‘just too much’ or ‘too intimate’. Put shortly, putting emotions on the table is institutionally embedded in the GP reflection context, but not always straightforward. Thus, we ask: ‘how do teachers and GP residents invite talk about emotions in educational reflection sessions?’.

Methods

In this study, we explored the Dutch phrase ‘raken, geraakt worden’ (being affected) as one interactional practice used to initiate emotion talk. We conducted a conversation analytic collection study of instances of this phenomenon based on 40 video recordings of hour-long ‘reflection sessions’ at the Dutch GP specialty training. During these sessions, approximately ten GPs in training discuss recent experiences from medical practice under supervision of one or two teachers.

Results

We found that participants orientated to the relevance of ‘being affected’ as a topic for discussion. Variations of the form ‘what affects you now?’ may contribute to putting emotions on the table; they can project a stepwise exploration of the emotional dimension of an experience. The ‘what affects you now’, often done in interrogative format doing a noticing, in combination with a request, is a powerful tool to instigate transformative sequences. The form is less effective to put emotions on the table when the topic shift it initiates is not grounded in previously presented personal stakes or displayed emotion.

Discussion

The study’s findings show how detailed interactional analysis of one sequentially structured practice can benefit education and contribute to theory on emotions and reflection. The mobilizing power of ‘what affects you’ can serve institutional purposes by doing topical work in relation to educational aims, while its power can also be deflated when prior talk does not project the relevance of unpacking the emotional dimension of an experience. Its interactional workings may translate to other helping contexts as well.
Validation of a community-based application of the Portuguese version of the survey on Social and Emotional Skills – Child/Youth Form
Authors: Castro Catarina , Barata Clara , Alexandre Joana , Colaço Carla
Published: August 2023

Introduction

Children and adolescents’ social and emotional skills have been gaining attention in diverse settings. With over 100 conceptual frameworks available, there is now a common move toward framing these skills as social and emotional learning (SEL), assuming that they are not only amiable to development, but also malleable to change as a product of intervention. As such, there is a strong need for a comprehensive measure to effectively evaluate such skills, validated for different age groups in children and young people, and applicable to both educational contexts and community settings.

Methods

This paper presents the validation of the Portuguese adaptation of the Child/Youth form of the Survey on Social and Emotional Skills (SSES), in the scope of the Gulbenkian Academies for Knowledge initiative with a sample of 7,831 participants between 8 and 17 years old (M = 11.79, SD = 2.94).

Results

Results show that the measure has good internal consistency and sensitivity, while also being sensitive to change over time. Preliminary factor analysis shows promise, although further research is necessary.

Discussion

Discussion reflects on the value of the Child/Youth form of the SSES as a comprehensive measure to be used by community and educational professionals to monitor skill development and improve their work on SEL.
The personality puzzle: a comprehensive analysis of its impact on three buying behaviors
Authors: Aquino Sibele D. , Lins Samuel
Published: August 2023

This study aimed at examining the role of personality traits in impulsive buying, compulsive buying, and panic buying simultaneously during the COVID-19 pandemic. At the beginning of the third confinement announced by the Portuguese government, 485 Portuguese answered in this study, mean age of 41.9 years (min = 18, max = 84; SD = 12.9), and 29.9% were men. Analyzes were carried out to investigate the association of Big Five’s personality factors with impulsive buying, compulsive buying, and panic buying. Results showed that the three buying behaviors under study have significant and positive correlations between them, and they also correlate with different personality traits. The association of each Big Five factor on buying behaviors differed. While conscientiousness was negatively and openness was positively associated with impulsive buying, conscientiousness was negatively associated with compulsive buying, agreeableness was positively associated with panic buying, and neuroticism correlated positively with all consumer behaviors. Understanding the personality traits that contribute to the development of a disorder may provide valuable insight into preventive measures and effective treatment approaches for some debilitating disorders. This study opens ways for investigating impulsive buying and compulsive buying by relating them to panic buying. It discusses the three different buying behaviors during the COVID-19 pandemic and future consumer research directions involving other variables.
Individually tailored dosage regimen of full-spectrum Cannabis extracts for autistic core and comorbid symptoms: a real-life report of multi-symptomatic benefits
Authors: Montagner Patrícia Soares Silva , Medeiros Wesley , da Silva Leandro Cruz Ramires , Borges Clarissa Nogueira , Brasil-Neto Joaquim , de Deus Silva Barbosa Vinícius , Caixeta Fabio V. , Malcher-Lopes Renato
Published: August 2023

Autism Spectrum Disorders (ASD) may significantly impact the well-being of patients and their families. The therapeutic use of cannabis for ASD has gained interest due to its promising results and low side effects, but a consensus on treatment guidelines is lacking. In this study, we conducted a retrospective analysis of 20 patients with autistic symptoms who were treated with full-spectrum cannabis extracts (FCEs) in a response-based, individually-tailored dosage regimen. The daily dosage and relative proportions of cannabidiol (CBD) and tetrahydrocannabinol (THC) were adjusted based on treatment results following periodic clinical evaluation. Most patients (80%) were treated for a minimum of 6 months. We have used a novel, detailed online patient- or caregiver-reported outcome survey that inquired about core and comorbid symptoms, and quality of life. We also reviewed patients’ clinical files, and no individual condition within the autistic spectrum was excluded. This real-life approach enabled us to gain a clearer appraisal of the ample scope of benefits that FCEs can provide for ASD patients and their families. Eighteen patients started with a CBD-rich FCE titrating protocol, and in three of them, the CBD-rich (CBD-dominant) FCE was gradually complemented with low doses of a THC-rich (THC-dominant) FCE based on observed effects. Two other patients have used throughout treatment a blend of two FCEs, one CBD-rich and the other THC-rich. The outcomes were mainly positive for most symptoms, and only one patient from each of the two above-mentioned situations displayed important side effects one who has used only CBD-rich FCE throughout the treatment, and another who has used a blend of CBD-Rich and THC-rich FCEs. Therefore, after FCE treatment, 18 out of 20 patients showed improvement in most core and comorbid symptoms of autism, and in quality of life for patients and their families. For them, side effects were mild and infrequent. Additionally, we show, for the first time, that allotriophagy (Pica) can be treated by FCEs. Other medications were reduced or completely discontinued in most cases. Based on our findings, we propose guidelines for individually tailored dosage regimens that may be adapted to locally available qualified FCEs and guide further clinical trials.
Mental health and use of Medicare Benefits Schedule follow-up mental health services by Indigenous people in Australia during the COVID-19 pandemic
Authors: Usher Kim , Jackson Debra , Peng Wenbo , Amarasena Suruchi , McCowan Debbie , Miller Joe , Cashman Belinda , Sibbritt David
Published: August 2023

Background

Mental health care has declined during the COVID-19 pandemic as has attendance for preventive mental health health services. This study aimed to investigate trends in all types of mental health service claims identified in an Indigenous-specific health assessment for Indigenous people before and during COVID-19.

Methods

We conducted an analysis of Medicare Benefits Scheme (MBS) mental health service items (Items 81,325 and 81,355), to investigate the trends in all types of mental health service claims specifically intended for Indigenous people of Australia. Data were analysed using descriptive statistics, including the total annual numbers of Indigenous peoples’ mental health service claims cross-tabulated by age groups and gender, between the calendar years 2017–2021. Multivariable Poisson regression modelling was used to determine associations that were statistically significant.

Results

Our results indicate an overall rise in MBS claims for mental health follow-up services during 2019–2020 followed by a decline in 2020–2021. In addition, there was an overall decline in claims for follow-up psychology services across the time period 2019–2021.

Conclusion

We found a significant decline in MBS items specific to follow-up mental health services (MBS Items 81,325 and 81,355) for Indigenous people in Australia suggesting a decline in attendance for mental health service follow-up which in turn may indicate a deficit in mental health care during the COVID-19 pandemic, an issue that may lead to poorer mental health outcomes in the future. Further research is needed to understand whether these changes were due to the impact of the COVID-19 pandemic or other factors.
Evaluation of online videos to engage viewers and support decision-making for COVID-19 vaccination: how narratives and race/ethnicity enhance viewer experiences
Authors: Schuh Holly B. , Rimal Rajiv N. , Breiman Robert F. , Orton Peter Z. , Dudley Matthew Z. , Kao Lee-Sien , Sargent Rikki H. , Laurie Shaelyn , Weakland Leo F. , Lavery James V. , Orenstein Walter A. , Brewer Janesse , Jamison Amelia M. , Shaw Jana , Josiah Willock Robina , Gust Deborah A. , Salmon Daniel A.
Published: August 2023

Background

Vaccine hesitancy has hampered the control of COVID-19 and other vaccine-preventable diseases.

Methods

We conducted a national internet-based, quasi-experimental study to evaluate COVID-19 vaccine informational videos. Participants received an informational animated video paired with the randomized assignment of (1) a credible source (differing race/ethnicity) and (2) sequencing of a personal narrative before or after the video addressing their primary vaccine concern. We examined viewing time and asked video evaluation questions to those who viewed the full video.

Results

Among 14,235 participants, 2,422 (17.0%) viewed the full video. Those who viewed a personal story first (concern video second) were 10 times more likely to view the full video (p < 0.01). Respondent–provider race/ethnicity congruence was associated with increased odds of viewing the full video (aOR: 1.89, p < 0.01). Most viewers rated the informational video(s) to be helpful, easy to understand, trustworthy, and likely to impact others' vaccine decisions, with differences by demographics and also vaccine intentions and concerns.

Conclusion

Using peer-delivered, personal narrative, and/or racially congruent credible sources to introduce and deliver vaccine safety information may improve the openness of vaccine message recipients to messages and engagement.
A pragmatic clinical effectiveness trial of a novel alternative to punishment for school-based substance use infractions: study protocol for the iDECIDE curriculum
Authors: Gray Caroline A. , Iroegbulem Vanessa , Deming Brooklyn , Butler Rebecca , Howell Dan , Pascale Michael P. , Bodolay Alec , Potter Kevin , Turncliff Amy , Lynch Stacey , Whittaker Jennie , Ward Julia , Maximus Devin , Pachas Gladys N. , Schuster Randi M.
Published: August 2023

Background

Adolescents who use alcohol and other drugs on school campuses are at heightened risk for adverse consequences to their health and wellbeing. Schools have historically turned to punitive approaches as a first-line response to substance use. However, punishment is an ineffective deterrent for substance use and may cause harm and increase inequities. iDECIDE (Drug Education Curriculum: Intervention, Diversion, and Empowerment) was developed as a scalable and youth-centered drug education and diversion program that can be used as a skills-based alternative to punishment. We aim to evaluate the effectiveness of the iDECIDE curriculum as an alternative to punishment (ATP) for school-based substance use infractions in the context of a large pragmatic clinical effectiveness study.

Methods

We will conduct a Type 1, hybrid effectiveness-implementation trial. Using a stepped wedge design with approximately 90 middle and high schools in Massachusetts, we will randomly allocate the timing of implementation of the iDECIDE curriculum compared to standard disciplinary response over approximately 36 months. We will test the overarching hypothesis that student-level outcomes (knowledge of drug effects and attitudes about substance use; frequency of substance use; school connectedness) improve over time as schools transition from a standard disciplinary response to having access to iDECIDE. The secondary aims of this trial are to (1) explore whether change in student-level outcomes vary according to baseline substance use, number of peers who use alcohol or other drugs, age, gender, and school urbanicity, and (2) determine the acceptability and feasibility of the iDECIDE curriculum through qualitative stakeholder interviews.

Discussion

Substance use continues to be a major and rapidly evolving problem in schools. The importance of moving away from punishment to more restorative approaches is widely accepted; however, scalable alternatives have not yet been identified. This will be the first study to our knowledge to systematically evaluate an ATP for students who violate the school substance use policy and is well poised to have important implications for policy making.
A rapid literature review on the health-related outcomes of long-term person-centered care models in adults with chronic illness
Authors: Cano Fátima , Alves Elisabete , João Ana , Oliveira Henrique , Pinho Lara Guedes , Fonseca César
Published: August 2023

Introduction

The strong association between age and the increasing prevalence of chronic diseases, makes it imperative to promote self-care throughout life. Systematic knowledge on the health findings of person-centered care models may contribute to designing effective healthcare strategies to promote empowerment for self-care in long-term care.

Objective

To assess the association between the implementation of person-centered care models that promote self-care training in long-term care and health-related outcomes, among adults with chronic illness.

Methods

A rapid review of the literature was performed following the Cochrane rapid review methodology. The electronic databases CINAHL, MedicLatina, MEDLINE, and Psychology and Behavioral Sciences Collection were searched for randomized experimental studies, published between 2017 and 2022, that implemented interventions based on person-centered models to promote self-care in adults aged ≥18 years with chronic diseases and needing long-term health care. Verification of the eligibility of the articles and the extraction of data were performed by two independent investigators. Quantitative data on the health-related variables assessed were collected and, through narrative synthesis, health outcomes were grouped into individual, institutional and societal levels.

Results

Eight studies, mostly conducted in European countries, were included. All satisfied more than 60% of the methodological quality score. A large variability among studies was found regarding the number of participants, the data collection period and duration of the intervention, the samples selected and the care model implemented. A high number of health-related outcomes (n = 17) were analyzed in the studies, using 52 different instruments. The main health-related outcomes were multidimensional, with implications at the individual, institutional and societal levels. The promotion of overall health and wellbeing (n = 4), the implementation of patient-centered care models (n = 1), the positive and more frequent interactions with health professionals (2), the decrease on staff psychosocial distress (n = 1), and the absence of added costs (n = 1), while improving family caregivers’ skills (n = 1) were the main health-related outcomes described.

Conclusion

There is a need to develop robust experimental studies focused on the views and experiences of all stakeholders and conducted in different countries and cultures. Short-, medium- and long-term health outcomes should be measured using internationally accepted and validated scales for chronic patients.
Dolenc approach for clipping of giant C6 and C7 segment aneurysms of the internal carotid artery
Authors: Zhang Hongwei , Liu Wei , Gu Yan , Li Aimin , Zhang Dong
Published: August 2023

Objective

Surgical treatment for giant aneurysms of the ICA-ophthalmic segment (C6) and communicating artery segment (C7) is a challenge for neurosurgeons because of their high risks and poor outcomes. We aim to explore the advantages and disadvantages of the Dolenc approach in the treatment of giant C6–C7 segment aneurysms.

Methods

We retrospectively reviewed the clinical data of 13 cases with giant C6 aneurysms and 4 cases with giant C7 aneurysms treated with the Dolenc approach.

Results

All 17 cases of aneurysms were clipped successfully using the Dolenc approach, of which, 1 case with ipsilateral MCA occlusion underwent extracranial-intracranial artery bypass after the aneurysm clipping. Regarding clinical outcomes, six out of nine cases with preoperative visual impairment improved after surgery, two cases saw no change, and one case deteriorated. Of all the cases, one had new-onset vision loss, four had new-onset oculomotor paralysis, three had surgical side cerebral infarction, and two had diabetes insipidus. DSA or CTA examination within 2 weeks after surgery showed that all aneurysms were completely clipped without residual. After a follow-up of 9–12 months, 17 patients were evaluated based on GOS and CTA examination. A total of 14 cases had GOS 5 scores, 2 cases had GOS 4 scores, 1 case had GOS 2 scores, and no cases had death. None of the patients had recurrence based on CTA examinations.

Conclusion

Through the Dolenc approach, we could achieve more operation space and expose clinoid segments for temporary occlusion. Therefore, the Dolenc approach was shown to be a safe, effective, and feasible treatment for giant C6–C7 aneurysms.
Feasibility of an endoscope-dominated side-to-end hypoglossal-facial anastomosis: an anatomical study
Authors: Yang Xiaobing , Man Dulegeqi , Yang Yang , Li Xingang
Published: August 2023

Objective

A surgical simulation of an endoscope-dominated side-to-end hypoglossal-facial anastomosis was performed to evaluate the feasibility.

Methods

Eight anatomical cadaver heads (16 sides) were recruited. The steps in conventional procedures were abbreviated or omitted. A facial nerve was first harvested near its external genu and was used for a side-to-end hypoglossal-facial anastomosis. The stump of the used facial nerve was truncated and recycled immediately caudal to the facial recess in another anastomosis and then recycled again at the stylomastoid foramen. As a recycled stump becomes too short to ensure a side-to-end anastomosis, the hypoglossal nerve was transected in situ, and an endoscopic end-to-end hypoglossal-facial anastomosis was attempted. Surgical simulation and quantitative measurement methods were used to analyze the anastomosis effects of different harvested sites of the facial nerve.

Results

Several steps in the conventional procedures provide little benefit in endoscopic surgery. A facial nerve stump recycled at the stylomastoid foramen is too short to ensure a tensionless side-to-end anastomosis. An endoscopic end-to-end hypoglossal-facial anastomosis was feasible, although it required more time than the classical microsurgical anastomosis. The greater agility of an endoscope enables the conventional surgical steps to be overlapped or interweaved into the procedure.

Conclusions

The multiple surgical fields and ability to manipulate the viewpoint provided by an endoscope have brought about breakthroughs in classical surgical paradigms. In addition, it is best to choose the sites of the facial nerve harvested near the external genu. If unavailable, an alternative section site could be selected immediately caudal to the facial recess, but cannot be distal to the stylomastoid foramen. The length of the stump should be individualized and preferably optimized with a nerve stimulator.
A study on the impact of double external shocks on Chinese wholesale pork prices
Authors: Yu Rui , Yang Xiaoli , Mu Endong
Published: August 2023

Introduction

Fluctuation in pork prices has always been a focus of academic attention. This paper examines the impact of double external shocks on pork prices, to provide reference for the impact of future outbreaks on the pork market.

Methods

This paper constructs a natural experiment based on the time and regional differences in the occurrence of the epidemics. Double difference models and triple difference models are used to identify the impacts of African swine fever and COVID-19 on Chinese pork prices.

Results

The results found that both African swine fever and COVID-19 positively affected pork prices, but African swine fever had a greater degree of impact; before the COVID-19 epidemic, African swine fever caused a more significant increase in pork prices; the impact of a single African swine fever shock was greater than the double shocks.

Discussion

The COVID-19 epidemic may have curbed the further increase in pork prices, due to the decreased market consumption demand caused by the epidemic.
Acute exercise in a hot environment increases heat shock protein 70 and peroxisome proliferator-activated receptor γ coactivator 1α mRNA in Thoroughbred horse skeletal muscle
Authors: Ebisuda Yusaku , Mukai Kazutaka , Takahashi Yuji , Yoshida Toshinobu , Kawano Aoto , Matsuhashi Tsubasa , Miyata Hirofumi , Kuwahara Masayoshi , Ohmura Hajime
Published: August 2023

Heat acclimatization or acclimation training in horses is practiced to reduce physiological strain and improve exercise performance in the heat, which can involve metabolic improvement in skeletal muscle. However, there is limited information concerning the acute signaling responses of equine skeletal muscle after exercise in a hot environment. The purpose of this study was to investigate the hypothesis that exercise in hot conditions induces greater changes in heat shock proteins and mitochondrial-related signaling in equine skeletal muscle compared with exercise in cool conditions. Fifteen trained Thoroughbred horses [4.6 ± 0.4 (mean ± SE) years old; 503 ± 14 kg] were assigned to perform a treadmill exercise test in cool conditions [COOL; Wet Bulb Globe Temperature (WBGT), 12.5°C; n = 8] or hot conditions (HOT; WBGT, 29.5°C; n = 7) consisting of walking at 1.7 m/s for 1 min, trotting at 4 m/s for 5 min, and cantering at 7 m/s for 2 min and at 90% of VO2max for 2 min, followed by walking at 1.7 m/s for 20 min. Heart rate during exercise and plasma lactate concentration immediately after exercise were measured. Biopsy samples were obtained from the middle gluteal muscle before and at 4 h after exercise, and relative quantitative analysis of mRNA expression using real-time RT-PCR was performed. Data were analyzed with using mixed models. There were no significant differences between the two groups in peak heart rate (COOL, 213 ± 3 bpm; HOT, 214 ± 4 bpm; p = 0.782) and plasma lactate concentration (COOL, 13.1 ± 1.4 mmoL/L; HOT, 17.5 ± 1.7 mmoL/L; p = 0.060), while HSP-70 (COOL, 1.9-fold, p = 0.207; HOT, 2.4-fold, p = 0.045), PGC-1α (COOL, 3.8-fold, p = 0.424; HOT, 8.4-fold, p = 0.010), HIF-1α (COOL, 1.6-fold, p = 0.315; HOT, 2.2-fold, p = 0.018) and PDK4 (COOL, 7.6-fold, p = 0.412; HOT, 14.1-fold, p = 0.047) mRNA increased significantly only in HOT at 4 h after exercise. These data indicate that acute exercise in a hot environment facilitates protective response to heat stress (HSP-70), mitochondrial biogenesis (PGC-1α and HIF-1α) and fatty acid oxidation (PDK4).
Intestinal Donation and Utilization: Single-Center Analysis Within Eurotransplant
Authors: Clarysse Mathias , Vanuytsel Tim , Canovai Emilio , Monbaliu Diethard , Ceulemans Laurens J. , Pirenne Jacques
Published: August 2023

Intestinal donor criteria are classically kept strict, thereby limiting donor supply. Indications for intestinal transplantation (ITx) are rare, but improved outcome and new emerging indications lead to increased demand and relaxing donor criteria should be considered. We sought to compare the donor criteria of intestines transplanted at our center with predefined (per protocol) criteria, and to determine how relaxing donor criteria could impact the potential donor pool. Donor criteria used in 22 consecutive ITx at our center between 2000 and 2020 were compared with predefined criteria. Next, multiorgan donors effectively offered by our Donor Network to Eurotransplant between 2014 and 2020 were retrospectively screened, according to predefined and effectively used intestinal donation criteria. Finally, utilization rate of offered intestines was calculated. In our ITx series, the effectively used donor criteria were less strict than those initially predefined. With these relaxed criteria, a favorable 5-year graft/patient survival of 75% and 95%, respectively was reached. Applying these relaxed criteria would lead to a 127% increase in intestinal offers. Paradoxically, 70% of offered intestines were not used. In conclusion, a significant increase in intestinal donation could be obtained by relaxing donor criteria, while still achieving excellent outcome. Offered intestines are underutilized.
Control of Cowl Shock/Boundary Layer Interaction in Supersonic Inlet Based on Dynamic Vortex Generator
Authors: Wang Mengge , Wang Ziyun , Zhang Yue , Cheng Daishu , Tan Huijun , Wang Kun , Gao Simin
Published: August 2023

A shock wave/boundary layer interaction (SWBLI) is a common phenomenon in supersonic inlet flow, which can significantly degrade the aerodynamic performance of the inlet by inducing boundary layer separation. To address this issue, in this paper, we propose the use of a dynamic vortex generator to control the SWBLI in a typical supersonic inlet. The unsteady simulation method based on dynamic grid technology was employed to verify the effectiveness of the proposed method of control and investigate its mechanism. The results showed that, in a duct of finite width at the inlet, the SWBLI generated complex three-dimensional (3D) flow structures with remarkable swirling properties. At the same time, vortex pairs were generated close to the side wall as a result of its presence, and this led to the intensification of transverse flow and, in turn, the formation of a complex 3D structure of the flow of the separation bubble. The dynamic vortex generator induced oscillations of variable intensity in the vortex system in the supersonic boundary layer that enhanced the mixing between the boundary layer flow and the mainstream. Meanwhile, the unique effects of “extrusion” and “suction” in the oscillation process continued to charge the airflow, and the distribution of velocity in the boundary layer significantly improved. As the oscillation frequency of the vortex generator increased, its charging effect on low-velocity flow in the boundary layer increased, and its control effect on the flow field of the SWBLI became more pronounced. The proposed method of control reduced the length of the separation bubble by 31.76% and increased the total pressure recovery coefficient at the inlet by 6.4% compared to the values in the absence of control.
Optimization Research on the Heat Transfer Capacity of an Aircraft Fuel Thermal Management System
Authors: Zhang Qidong , Lin Guiping , Guo Jinghui , Jin Haichuan , Zhang Qiming
Published: August 2023

The thermal management system (TMS) for aircraft fuel is a critical component of integrated TMSs in aircraft. As such, its optimal design is necessary to ensure the efficient completion of flight missions. This study presents the model building of a numerical simulation model for the fuel TMS, with the objective of minimizing fuel return flow. Sensitivity analysis was performed using variance analysis. The genetic algorithm was utilized for the optimization of the model building, taking into consideration the system’s geometric structure and performance parameters, which include the pipe length, the ram air-fuel HX’s efficiency, and the ram air’s volume flow rate in the ram air cooling subsystem, as design variables. The optimization solution for system design variables yielded a design scheme with the highest working efficiency for the fuel TMS. In this paper, the genetic algorithm in AMEsim software is adopted, which can also effectively optimize the design parameters and achieve the optimization objective. Compared with the original TMS structure, the heat dissipation capacity of the fuel TMS is improved and reduced the return fuel flow by 67.4% after the optimization of system structure parameters.
Effect of Combustor Outlet Geometry on Operating Characteristics of Disk-Shaped Rotating Detonation Engine
Authors: Xia Zhenjuan , Ma Hu , Ge Gaoyang , He Yong , Zhou Changsheng
Published: August 2023

A disk-shaped rotating detonation engine with H2/air mixture was tested to identify the impact of combustor outlet geometry on the engine’s operating characteristics. Three combustor outlet diameters and five outlet lengths are employed in the experiments. Results show that with the increase of combustor convergent ratio, the propagation stability of the rotating detonation wave decreases, and the propagation velocity and pressure peak decrease slightly. When the convergent ratio increases to a certain value (1.70 in this study), a “platform zone” with a lower pressure value appears before the sharp rise of the dynamic pressure curve. The propagation mode varies with the increase of mass flow rate at different convergent ratios. As the mass flow rate increases, the wave head number in the combustor increases. But the change rule of propagation mode with mass flow rate is greatly affected by convergent ratio. Increasing the convergent ratio is conducive to the formation of multi-wave modes, and the critical mass flow rate for mode transition drops sharply. When the convergent ratio increases to 1.70, the unstable asymmetric dual-wave mode is obtained. With the increase in the convergent ratio, the engine’s operating range and operating stability decrease significantly. Finally, changing the combustor outlet length has little influence on the engine’s operating characteristics and detonation-wave parameters.
Adaptive Robust Time-Varying Formation Control of Quadrotors under Switching Topologies: Theory and Experiment
Authors: Zhao Ziqian , Zhu Ming , Qin Jiazheng
Published: August 2023

This paper investigates a practical time-varying formation control method for quadrotors subjected to disturbances, uncertainties, and switching-directed topologies. A fully distributed formation control scheme is proposed using a linear-velocity independent position controller (LVIPC) and a nonsingular terminal sliding mode attitude controller (NTSMAC). A distributed observer is adopted to eliminate the measurement of linear-velocity states, and only local neighbor states are needed to realize formation flight. A time-varying nonsingular terminal sliding mode manifold is designed to suppress the reaching phase and ensure the finite-time convergence. Adaptive estimators are employed to remove the reliance on the prior knowledge of the upper bound of lumped uncertainties. It is then proven that all the closed-loop signals are bounded under the proposed method. Comparative experimental results based on a practical outdoor hardware solution are presented to confirm the effectiveness of the suggested control algorithm.
Morpho-Physiological and Antioxidative Responses of Wheat Seedlings to Different Forms of Selenium
Authors: Vuković Popović Ana , Štolfa Čamagajevac Ivna , Vuković Rosemary , Matić Magdalena , Gupta Dharmendra K. , Lončarić Zdenko
Published: August 2023

Selenium (Se) deficiency in human and animal nutrition is primarily due to low levels of Se in soils. It can be prevented by enriching crops, such as wheat, with Se through agronomic biofortification. Although Se is not essential for plants, it shows a dual effect on their metabolism depending on its concentration. This study aimed to elucidate the impact of five different concentrations (0.4, 4, 20, 40, and 400 mg/kg) of selenate and selenite on the oxidative status and antioxidative response of wheat (Triticum aestivum L., cv. Kraljica) shoots and roots. According to morpho-physiological analyses, selenite was found to have a lower toxicity threshold than selenate. The measurement of oxidative stress biomarkers showed that Se did not cause oxidative damage to wheat seedlings due to the activation of detoxification mechanisms at the biochemical level, which depended on the type of tissue, concentration, and form of applied Se. Treatment with 20 mg/kg of selenate can be recommended for wheat seedling biofortification due to a sufficient increase in Se accumulation in shoots without signs of toxicity. These results contribute to a better understanding of wheat seedlings’ physiological and biochemical responses to Se and the development of more effective biofortification strategies.
Describing Behavior Sequences of Fattening Pigs Using Process Mining on Video Data and Automated Pig Behavior Recognition
Authors: Melfsen Andreas , Lepsien Arvid , Bosselmann Jan , Koschmider Agnes , Hartung Eberhard
Published: August 2023

This study aimed to demonstrate the application of process mining on video data of pigs, facilitating the analysis of behavioral patterns. Video data were collected over a period of 5 days from a pig pen in a mechanically ventilated barn and used for analysis. The approach in this study relies on a series of individual steps to allow process mining on this data set. These steps include object detection and tracking, spatiotemporal activity recognition in video data, and process model analysis. Each step gives insights into pig behavior at different time points and locations within the pen, offering increasing levels of detail to describe typical pig behavior up to process models reflecting different behavior sequences for clustered datasets. Our data-driven approach proves suitable for the comprehensive analysis of behavioral sequences in conventional pig farming.
Detection and Classification of Citrus Fruit Infestation by Bactrocera dorsalis (Hendel) Using a Multi-Path Vis/NIR Spectroscopy System
Authors: Li Dapeng , Long Jiang , Tang Ziye , Han Longbo , Gong Zhongliang , Wen Liang , Peng Hailong , Wen Tao
Published: August 2023

In this study, a multi-path Vis/NIR spectroscopy system was developed to detect the presence of Bactrocera dorsalis (Hendel) infestations of citrus fruit. Spectra were acquired for 252 citrus fruit, 126 of which were infested. Two hundred and fifty-two spectra were acquired for modeling in their un-infested stage, slightly infested stage, and seriously infested stage. The location of the infestation is unclear, and considering the impact of the light path on the location of the infestation, each citrus fruit was tested in three orientations (i.e., fruit stalks facing upward (A), fruit stalks facing horizontally (B), and fruit stalks facing downward (C)). Classification models based on joint X-Y distance, multiple transmittance calibration, competitive adaptive reweighted sampling, and partial least squares discriminant analysis (SPXY-MSC-CARS-PLS-DA) were developed on the spectra of each light path, and the average spectra of the four light paths was calculated, to compare their performance in infestation classification. The results show the classification result changed with the light path and fruit orientation. The average spectra for each fruit orientation consistently gave better classification results, with overall accuracies of 92.9%, 89.3%, and 90.5% for orientations A, B, and C, respectively. Moreover, the best model had a Kappa value of 0.89, and gave 95.2%, 80.1%, and 100.0% accuracy for un-infested, slightly infested, and seriously infested citrus fruit. Furthermore, the classification results for infested citrus fruits were better when using the average spectra than using the spectrum of each single light path. Therefore, the multi-path Vis/NIR spectroscopy system is conducive to the detection of B. dorsalis infestation in citrus fruits.
Risk Cognition, Social Learning, and Farmers’ Adoption of Conservation Agriculture Technology
Authors: Ren Yaqin , Feng Hui , Gao Tianzhi
Published: August 2023

Soil degradation and declining soil fertility are prominent issues for sustainable agricultural development in China. Therefore, it is of great significance to promote the adoption rate of conservation agriculture technology. Risk cognition and technology adoption are closely related, but this perspective is rarely focused on, and it is essential to discuss the influence of social learning on the impact. The Loess Plateau is a representative area for promoting and implementing conservation agriculture techniques. By collecting face-to-face survey data from 1268 farmers in Shaanxi, Shanxi, and Ningxia provinces in China, this study used the binary probit model to examine the impact of risk cognition on the adoption of conservation agriculture technology and the influence of social learning on the impact. The results showed that risk cognition has a significant positive impact on the adoption of conservation agriculture technology; social learning significantly enhances the effect of risk cognition on farmers’ adoption of conservation agriculture technology. Both offline practical learning through “learning by doing” and online learning with ICT play an important moderating role in the impact; a high level of social learning enhances risk cognition to a greater extent and promotes enthusiasm for adopting conservation agriculture technology. Therefore, the value of farmers’ risk cognition should be considered in promoting and implementing conservation agriculture technology. Moreover, expanding offline and online social learning channels is crucial to improve farmers’ risk cognition and promote the adoption of conservation agriculture technology.