bluuebunny/crossref_metadata_2025 huggingface dataset:
A Network Pharmacology and Multi-Omics Combination Approach to Reveal the Effect of Strontium on Ca2+ Metabolism in Bovine Rumen Epithelial Cells
Authors: Tan Panpan , Zhao Chenxu , Dong Yong , Zhang Zixin , Mei Linshan , Kong Yezi , Zeng Fangyuan , Wen Yongqiang , Zhao Baoyu , Wang Jianguo
Published: May 2023

Strontium (Sr) belongs to the same group in the periodic table as calcium (Ca). Sr level can serve as an index of rumen Ca absorption capacity; however, the effects of Sr on Ca2+ metabolism are unclear. This study aims to investigate the effect of Sr on Ca2+ metabolism in bovine rumen epithelial cells. The bovine rumen epithelial cells were isolated from the rumen of newborn Holstein male calves (n = 3, 1 day old, 38.0 ± 2.8 kg, fasting). The half maximal inhibitory concentration (IC50) of Sr-treated bovine rumen epithelial cells and cell cycle were used to establish the Sr treatment model. Transcriptomics, proteomics, and network pharmacology were conducted to investigate the core targets of Sr-mediated regulation of Ca2+ metabolism in bovine rumen epithelial cells. The data of transcriptomics and proteomics were analyzed using bioinformatic analysis (Gene Ontology and Kyoto Encyclopedia of genes/protein). Quantitative data were analyzed using one-way ANOVA in GraphPad Prism 8.4.3 and the Shapiro–Wilk test was used for the normality test. Results presented that the IC50 of Sr treatment bovine rumen epithelial cells for 24 h was 43.21 mmol/L, and Sr increased intracellular Ca2+ levels. Multi-omics results demonstrated the differential expression of 770 mRNAs and 2436 proteins after Sr treatment; network pharmacology and reverse transcriptase polymerase chain reaction (RT-PCR) revealed Adenosylhomocysteine hydrolase-like protein 2 (AHCYL2), Semaphoring 3A (SEMA3A), Parathyroid hormone-related protein (PTHLH), Transforming growth factor β2 (TGF-β2), and Cholesterol side-chain cleavage enzyme (CYP11A1) as potential targets for Sr-mediated Ca2+ metabolism regulation. Together these results will improve the current comprehension of the regulatory effect of Sr on Ca2+ metabolism and pave a theoretical basis for Sr application in bovine hypocalcemia.
HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction
Authors: Djekic Saska , Vekic Jelena , Zeljkovic Aleksandra , Kotur-Stevuljevic Jelena , Kafedzic Srdjan , Zdravkovic Marija , Ilic Ivan , Hinic Sasa , Cerovic Milivoje , Stefanovic Milica , Mihajlovic Marija , Neskovic Aleksandar , Bogavac-Stanojevic Natasa
Published: May 2023

The aim of this multicentric study was to assess the impacts of oxidative stress, inflammation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1) activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In 69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within HDL) was estimated using the zymogram method. The STEMI patients had significantly lower proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1 within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013 and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small HDL3 particles and the altered pPON1 within HDL.
Apoptosis Genes as a Key to Identification of Inverse Comorbidity of Huntington’s Disease and Cancer
Authors: Bragina Elena Yu. , Gomboeva Densema E. , Saik Olga V. , Ivanisenko Vladimir A. , Freidin Maxim B. , Nazarenko Maria S. , Puzyrev Valery P.
Published: May 2023

Cancer and neurodegenerative disorders present overwhelming challenges for healthcare worldwide. Epidemiological studies showed a decrease in cancer rates in patients with neurodegenerative disorders, including the Huntington disease (HD). Apoptosis is one of the most important processes for both cancer and neurodegeneration. We suggest that genes closely connected with apoptosis and associated with HD may affect carcinogenesis. We applied reconstruction and analysis of gene networks associated with HD and apoptosis and identified potentially important genes for inverse comorbidity of cancer and HD. The top 10 high-priority candidate genes included APOE, PSEN1, INS, IL6, SQSTM1, SP1, HTT, LEP, HSPA4, and BDNF. Functional analysis of these genes was carried out using gene ontology and KEGG pathways. By exploring genome-wide association study results, we identified genes associated with neurodegenerative and oncological disorders, as well as their endophenotypes and risk factors. We used publicly available datasets of HD and breast and prostate cancers to analyze the expression of the identified genes. Functional modules of these genes were characterized according to disease-specific tissues. This integrative approach revealed that these genes predominantly exert similar functions in different tissues. Apoptosis along with lipid metabolism dysregulation and cell homeostasis maintenance in the response to environmental stimulus and drugs are likely key processes in inverse comorbidity of cancer in patients with HD. Overall, the identified genes represent the promising targets for studying molecular relations of cancer and HD.
A Current State of Proteomics in Adult and Pediatric Inflammatory Bowel Diseases: A Systematic Search and Review
Authors: Fabian Ondrej , Bajer Lukas , Drastich Pavel , Harant Karel , Sticova Eva , Daskova Nikola , Modos Istvan , Tichanek Filip , Cahova Monika
Published: May 2023

Inflammatory bowel diseases (IBD) are systemic immune-mediated conditions with predilection for the gastrointestinal tract and include Crohn’s disease and ulcerative colitis. Despite the advances in the fields of basic and applied research, the etiopathogenesis remains largely unknown. As a result, only one third of the patients achieve endoscopic remission. A substantial portion of the patients also develop severe clinical complications or neoplasia. The need for novel biomarkers that can enhance diagnostic accuracy, more precisely reflect disease activity, and predict a complicated disease course, thus, remains high. Genomic and transcriptomic studies contributed substantially to our understanding of the immunopathological pathways involved in disease initiation and progression. However, eventual genomic alterations do not necessarily translate into the final clinical picture. Proteomics may represent a missing link between the genome, transcriptome, and phenotypical presentation of the disease. Based on the analysis of a large spectrum of proteins in tissues, it seems to be a promising method for the identification of new biomarkers. This systematic search and review summarize the current state of proteomics in human IBD. It comments on the utility of proteomics in research, describes the basic proteomic techniques, and provides an up-to-date overview of available studies in both adult and pediatric IBD.
Biparametric Q Rung Orthopair Fuzzy Entropy Measure for Multi Criteria Decision Making Problem
Authors: Suri Gitesh , Svitenko Heorhii , Guleria Abhishek , Gandotra Neeraj , Saini Namita , Sałabun Wojciech
Published: May 2023

In this study we propose a measure of the entropy of the norm (R, S) for q-row orthopair fuzzy sets (qROFS). The proposed entropy measure is validated both theoretically and practically to ensure validity. We also propose a simple methodology for the purpose of solving a multi-criteria decision-analysis problems using the introduced entropy measure. This method takes into account different circumstances of criteria weights, such as unknown weights, as well as other cases when the weights are not fully known. Finally, a demonstration with numerical examples for the proposed entropy has been provided to show how to apply the novel methodologies.
Artificially Intelligent Readers: An Adaptive Framework for Original Handwritten Numerical Digits Recognition with OCR Methods
Authors: Jain Parth Hasmukh , Kumar Vivek , Samuel Jim , Singh Sushmita , Mannepalli Abhinay , Anderson Richard
Published: May 2023

Advanced artificial intelligence (AI) techniques have led to significant developments in optical character recognition (OCR) technologies. OCR applications, using AI techniques for transforming images of typed text, handwritten text, or other forms of text into machine-encoded text, provide a fair degree of accuracy for general text. However, even after decades of intensive research, creating OCR with human-like abilities has remained evasive. One of the challenges has been that OCR models trained on general text do not perform well on localized or personalized handwritten text due to differences in the writing style of alphabets and digits. This study aims to discuss the steps needed to create an adaptive framework for OCR models, with the intent of exploring a reasonable method to customize an OCR solution for a unique dataset of English language numerical digits were developed for this study. We develop a digit recognizer by training our model on the MNIST dataset with a convolutional neural network and contrast it with multiple models trained on combinations of the MNIST and custom digits. Using our methods, we observed results comparable with the baseline and provided recommendations for improving OCR accuracy for localized or personalized handwritten text. This study also provides an alternative perspective to generating data using conventional methods, which can serve as a gold standard for custom data augmentation to help address the challenges of scarce data and data imbalance.
Regularized Generalized Logistic Item Response Model
Authors: Robitzsch Alexander
Published: May 2023

Item response theory (IRT) models are factor models for dichotomous or polytomous variables (i.e., item responses). The symmetric logistic or probit link functions are most frequently utilized for modeling dichotomous or polytomous items. In this article, we propose an IRT model for dichotomous and polytomous items using the asymmetric generalistic logistic link function that covers a lot of symmetric and asymmetric link functions. Compared to IRT modeling based on the logistic or probit link function, the generalized logistic link function additionally estimates two parameters related to the asymmetry of the link function. To stabilize the estimation of item-specific asymmetry parameters, regularized estimation is employed. The usefulness of the proposed model is illustrated through simulations and empirical examples for dichotomous and polytomous item responses.
An Intelligent Conversational Agent for the Legal Domain
Authors: Amato Flora , Fonisto Mattia , Giacalone Marco , Sansone Carlo
Published: May 2023

An intelligent conversational agent for the legal domain is an AI-powered system that can communicate with users in natural language and provide legal advice or assistance. In this paper, we present CREA2, an agent designed to process legal concepts and be able to guide users on legal matters. The conversational agent can help users navigate legal procedures, understand legal jargon, and provide recommendations for legal action. The agent can also give suggestions helpful in drafting legal documents, such as contracts, leases, and notices. Additionally, conversational agents can help reduce the workload of legal professionals by handling routine legal tasks. CREA2, in particular, will guide the user in resolving disputes between people residing within the European Union, proposing solutions in controversies between two or more people who are contending over assets in a divorce, an inheritance, or the division of a company. The conversational agent can later be accessed through various channels, including messaging platforms, websites, and mobile applications. This paper presents a retrieval system that evaluates the similarity between a user’s query and a given question. The system uses natural language processing (NLP) algorithms to interpret user input and associate responses by addressing the problem as a semantic search similar question retrieval. Although a common approach to question and answer (Q&A) retrieval is to create labelled Q&A pairs for training, we exploit an unsupervised information retrieval system in order to evaluate the similarity degree between a given query and a set of questions contained in the knowledge base. We used the recently proposed SBERT model for the evaluation of relevance. In the paper, we illustrate the effective design principles, the implemented details and the results of the conversational system and describe the experimental campaign carried out on it.
Using Sentinel Plots to Monitor for Changes in Thrips Susceptibility to MON 88702 Cotton Containing the Cry51Aa2.834_16 Bt Protein
Authors: Yates-Stewart Ashley D. , Yorke Benjamin T. , Willse Alan , Fridley Jennifer , Head Graham P.
Published: May 2023

Transgenic Bt crops are important tools for growers to manage insect pests, but their durability is threatened by the evolution of insect resistance. Implementing a resistance monitoring program is essential to detect and mitigate resistance. For non-high-dose Bt crops, resistance monitoring is challenging, because insect control is not complete, so targeted insects and insect damage will be present even without resistance. Given these challenges, sentinel plots have been used to monitor for insect resistance to non-high-dose crops by assessing changes in the efficacy of a Bt crop over time relative to a non-Bt control. We optimized a sentinel plot resistance monitoring approach for MON 88702 ThryvOn™ cotton, a new non-high-dose Bt product targeting two sucking pest taxa—Lygus (L. lineolaris and L. hesperus) and thrips (Frankliniella fusca and F. occidentalis)—and report here on the thrips monitoring methods and results. Quantifying thrips immatures was the best metric to characterize the impact of the trait, with at least a 40–60% average reduction of thrips immatures on ThryvOn relative to the control cotton at all sites with higher thrips densities. These data can be used within a ThryvOn resistance monitoring program and represent a case study for establishing a resistance monitoring approach for a non-high-dose trait product.
Innovation in Acute Ischemic Stroke Patients over 80 y/o—A Retrospective Monocentric Study on Mechanical Thrombectomy of Consecutive Patients: Is Age an Adequate Selection Criterion?
Authors: Cernigliaro Massimiliano , Stanca Carmelo , Galbiati Andrea , Spinetta Marco , Coda Carolina , Negroni Davide , Laganà Domenico , Minici Roberto , Airoldi Chiara , Carriero Alessandro , Guzzardi Giuseppe
Published: May 2023

Background: Although it is clear that stroke is a time-dependent and age-associated disease, we still need more evidence regarding the efficacy and outcomes in elderly patients who were excluded from the first trials of mechanical thrombectomy. The aim of this study is to highlight patient characteristics, the timing of medical attention and therapy, successful recanalization, and functional outcomes in patients over 80 y/o who underwent mechanical thrombectomy at the Ospedale Maggiore della Carità di Novara (Hub) since endovascular stroke treatment was first started here. Methods: all 122 consecutive patients over 80 y/o at admission who underwent mechanical thrombectomy between 2017 and 2022 at our Hub center were retrospectively included in our database. A good functional outcome in these elderly patients was considered as the 90 days modified Rankin Scale (mRS) ≤ 3 and/or a decrease in functional status as ∆mRS ≤ 1 in order to interpret the results for patients with intact intellect and basal mRS > 3. Successful recanalization as a score of TICI ≥ 2b (Thrombolysis in Cerebral Infarction) was analyzed as a secondary outcome. Results: Good functional outcome (mRS ≤ 3 and/or ∆mRS ≤ 1) was observed in 45.90% (56/122). The rate of successful recanalization (TICI ≥ 2b) was 65.57% (80/122). Conclusion: Our data confirm that a good outcome in the elderly age group has a correlation with age; being younger, with a milder NIHSS (National Institutes of Health Stroke Scale) at the onset and with a lower pre-morbid mRS is statistically associated with a better outcome. However, age should not be a criterion to exclude older patients from mechanical thrombectomy. Decision-making should take into consideration the pre-morbid mRS and the severity of the stroke on the NIHSS scale, especially in the age group over 85 y/o.
Dapaglifozin on Albuminuria in Chronic Kidney Disease Patients with FabrY Disease: The DEFY Study Design and Protocol
Authors: Battaglia Yuri , Bulighin Francesca , Zerbinati Luigi , Vitturi Nicola , Marchi Giacomo , Carraro Gianni
Published: May 2023

Fabry disease (FD) is a rare genetic disorder caused by a deficiency in the α-galactosidase A enzyme, which results in the globotriaosylceramide accumulation in many organs, including the kidneys. Nephropathy is a major FD complication that can progress to end-stage renal disease if not treated early. Although enzyme replacement therapy and chaperone therapy are effective, other treatments such as ACE inhibitors and angiotensin receptor blockers can also provide nephroprotective effects when renal damage is also established. Recently, SGLT2 inhibitors have been approved as innovative drugs for treating chronic kidney disease. Thus, we plan a multicenter observational prospective cohort study to assess the effect of Dapagliflozin, a SGLT2 inhibitor, in FD patients with chronic kidney disease (CKD) stages 1–3. The objectives are to evaluate the effect of Dapagliflozin primarily on albuminuria and secondarily on kidney disease progression and clinical FD stability. Thirdly, any association between SGT2i and cardiac pathology, exercise capacity, kidney and inflammatory biomarkers, quality of life, and psychosocial factors will also be evaluated. The inclusion criteria are age ≥ 18; CKD stages 1–3; and albuminuria despite stable treatment with ERT/Migalastat and ACEi/ARB. The exclusion criteria are immunosuppressive therapy, type 1 diabetes, eGFR < 30 mL/min/1.73 m2, and recurrent UTIs. Baseline, 12-month, and 24-month visits will be scheduled to collect demographic, clinical, biochemical, and urinary data. Additionally, an exercise capacity and psychosocial assessment will be performed. The study could provide new insights into using SGLT2 inhibitors for treating kidney manifestations in Fabry disease.
Total or Subtotal Hysterectomy for the Treatment of Endometriosis: A Review
Authors: Alkatout Ibrahim , Mazidimoradi Afrooz , Günther Veronika , Salehiniya Hamid , Allahqoli Leila
Published: May 2023

Objective: The purpose of the review was to evaluate and compare outcomes after total or subtotal hysterectomy in women with endometriosis or adenomyosis. Methods: We searched four electronic databases: Medline (PubMed), Scopus, Embase, and Web of Science (WoS). The first aim of the study was to compare outcomes after total and subtotal hysterectomy in women with endometriosis, and the second aim was to compare the two procedures in women with adenomyosis. Publications that reported short- and long-term outcomes after total and subtotal hysterectomy were included in the review. The search was not subject to any limitation in terms of time or method. Results: After screening 4948 records, we included 35 studies published from 1988 to 2021; the studies were based on various methodologies. With regard to the first aim of the review, we found 32 eligible studies and divided these into the following four categories: postoperative short- and long-term outcomes, recurrence of endometriosis, quality of life and sexual function, and patient satisfaction after total or subtotal hysterectomy in women with endometriosis. Five investigations were deemed eligible for the second aim of the review. No differences were seen in terms of postoperative short- and long-term outcomes after subtotal or total hysterectomy in women with endometriosis or adenomyosis. Conclusions: Preservation or removal of the cervix in women with endometriosis or adenomyosis appears to have no effect on short- or long-term outcomes, recurrence of endometriosis, quality of life and sexual function, or patient satisfaction. Nevertheless, we lack randomized blinded controlled trials on these aspects. Such trials will be needed to enhance our comprehension of both surgical approaches.
Sex Dimorphism in Outcome of Trauma Patients Presenting with Severe Shock: A Multicenter Cohort Study
Authors: Van Wonderen Stefan F. , Pape Merel , Zuidema Wietse P. , Edwards Michael J. R. , Verhofstad Michael H. J. , Tromp Tjarda N. , Van Lieshout Esther M. M. , Bloemers Frank W. , Geeraedts Leo M. G.
Published: May 2023

Background: The objective of this study was to determine the association between sex and outcome among severely injured patients who were admitted in severe shock. Methods: A retrospective multicenter study was performed in trauma patients (Injury Severity Score (ISS) ≥ 16) aged ≥ 16 presenting with severe shock (Shock Index > 1.3) over a 4-year period. To determine if sex was associated with mortality, Intensive Care Unit (ICU) admission, mechanical ventilation, blood transfusion and in-hospital complications, multivariable logistic regressions were performed. Results: In total, 189 patients were admitted to the Emergency Department in severe shock. Multivariable logistic regression analysis showed that female sex was independently associated with a decreased likelihood of acute kidney injury (OR 0.184; 95% CI 0.041–0.823; p = 0.041) compared to the male sex. A significant association between female sex and mortality, ICU admission, mechanical ventilation, other complications and packed red blood cells transfusion after admission could not be confirmed. Conclusion: Female trauma patients in severe shock were significantly less likely to develop AKI during hospital stay. These results could suggest that female trauma patients may manifest a better-preserved physiologic response to severe shock when compared to their male counterparts. Prospective studies with a larger study population are warranted.
The Association between Dysnatraemia during Hospitalisation and Post-COVID-19 Mental Fatigue
Authors: Salvato Gerardo , Inglese Elvira , Fazia Teresa , Crottini Francesco , Crotti Daniele , Valentini Federica , Palmas Giulio , Bollani Alessandra , Basilico Stefania , Gandola Martina , Gelosa Giorgio , Gentilini Davide , Bernardinelli Luisa , Stracciari Andrea , Scaglione Francesco , Agostoni Elio Clemente , Bottini Gabriella
Published: May 2023

COVID-19 may induce short- and long-term cognitive failures after recovery, but the underlying risk factors are still controversial. Here, we investigated whether (i) the odds of experiencing persistent cognitive failures differ based on the patients’ disease course severity and sex at birth; and (ii) the patients’ electrolytic profile in the acute stage represents a risk factor for persistent cognitive failures. We analysed data from 204 patients suffering from COVID-19 and hospitalised during the first pandemic wave. According to the 7-point WHO-OS scale, their disease course was classified as severe or mild. We investigated the presence of persistent cognitive failures collected after hospital discharge, while electrolyte profiles were collected during hospitalisation. The results showed that females who suffered from a mild course compared to a severe course of COVID-19 had a higher risk of presenting with persistent mental fatigue after recovery. Furthermore, in females who suffered from a mild course of COVID-19, persistent mental fatigue was related to electrolyte imbalance, in terms of both hypo- and hypernatremia, during hospitalisation in the acute phase. These findings have important implications for the clinical management of hospitalised COVID-19 patients. Attention should be paid to potential electrolyte imbalances, mainly in females suffering from mild COVID-19.
Oxidized Regenerated Cellulose versus Polyglycolic Acid for Pleural Coverage in Pneumothorax Surgery
Authors: Woo Wongi , Kim Bong Jun , Moon Duk Hwan , Kang Du-young , Lee Sungsoo , Oh Tae Yun
Published: May 2023

Objectives: Although surgical intervention for spontaneous pneumothorax (SP) reduces the recurrence rate, thoracoscopic surgery is associated with greater postoperative recurrence rates than open thoracotomy. A polyglycolic acid (PGA) sheet or oxidized regenerated cellulose (ORC) mesh can therefore be used for additional coverage after thoracoscopic surgery, and this study compared the clinical impacts of these two materials. Methods: From 2018 to 2020, 262 thoracoscopic surgeries for primary SP were performed, of which 125 patients were enrolled in this study, and 48 and 77 patients received ORC and PGA coverage, respectively. The clinical characteristics and surgical procedures were reviewed, and the recurrence rates were compared. To obtain more comprehensive evidence, we performed a literature review and meta-analysis comparing ORC and PGA coverage. Results: There were no significant differences in patient characteristics between the two groups. Operating time was slightly shorter in the ORC group than in the PGA group (p = 0.008). The pneumothorax recurrence rate was similar in both groups (PGA: 10.4%, ORC: 6.2%, p = 0.529), but the recurrence-free interval was significantly longer (p = 0.036) in the ORC (262 days) than in the PGA (48.5 days) group. The literature review identified three relevant studies, and the meta-analysis revealed no difference in pneumothorax recurrence rate between the two coverage materials. Conclusions: The two visceral pleural coverage materials, PGA and ORC, did not show significant differences in postoperative pneumothorax recurrence. Therefore, if applied appropriately, the choice of material between ORC and PGA for thoracoscopic pneumothorax surgery does not have a significant impact on the clinical outcome.
Nagor Impleo Round Silicone Gel Breast Implants: Early Outcome Analysis after 340 Primary Breast Augmentations
Authors: Zaussinger Maximilian , Duscher Dominik , Huemer Georg M.
Published: May 2023

For decades, implant-based breast augmentation has been one of the most performed surgical procedures for cosmetic purposes around the world. Hence, novel manufactured implants should be critically investigated to prove them safe and effective. Here, the authors describe the first independently conducted clinical study on Nagor Impleo textured round breast implants. For this retrospective study, outcomes of 340 consecutive female patients undergoing primary cosmetic breast augmentation were analyzed. Demographic and surgical data as well as outcomes and complications were evaluated. Furthermore, a survey concerning effectiveness and aesthetic satisfaction after breast augmentation was examined. All 680 implants were placed in a submuscular plane with incisions at the inframammary fold. The main indications for surgery were hypoplasia and hypoplasia with asymmetry. The mean implant volume was 390 cc and the main type of projection was high profile. The most common complications were hematoma and capsular contracture (0.9 percent, respectively). The overall revision rate for complications was 2.4%. Additionally, almost all patients showed increased quality of life and aesthetic satisfaction after a breast augmentation. Hence, all patients would undergo breast augmentation again with these newly launched devices. Nagor Impleo implants demonstrate a low complication rate and high safety profile. Although high aesthetic satisfaction and quality of life results were achieved, analysis of an even larger series over a longer period of time would be beneficial to evaluate the reliability of this implant.
Psychometric Properties of the German Version of the Quality of Life after Brain Injury Scale for Kids and Adolescents (QOLIBRI-KID/ADO) Using Item Response Theory Framework: Results from the Pilot Study
Authors: Zeldovich Marina , Cunitz Katrin , Greving Sven , Muehlan Holger , Bockhop Fabian , Krenz Ugne , Timmermann Dagmar , Koerte Inga K. , Rojczyk Philine , Roediger Maike , Lendt Michael , von Steinbuechel Nicole
Published: May 2023

Health-related quality of life (HRQOL) is an important indicator for recovery after pediatric TBI. To date, there are a few questionnaires available for assessing generic HRQOL in children and adolescents, but there are not yet any TBI-specific measures of HRQOL that are applicable to pediatric populations. The aim of the present study was to examine psychometric characteristics of the newly developed Quality of Life After Brain Injury Scale for Kids and Adolescents (QOLIBRI-KID/ADO) questionnaire capturing TBI-specific HRQOL in children and adolescents using an item response theory (IRT) framework. Children (8–12 years; n = 152) and adolescents (13–17 years; n = 148) participated in the study. The final version of the QOLIBRI-KID/ADO, comprising 35 items forming 6 scales, was investigated using the partial credit model (PCM). A scale-wise examination for unidimensionality, monotonicity, item infit and outfit, person homogeneity, and local independency was conducted. The questionnaire widely fulfilled the predefined assumptions, with a few restrictions. The newly developed QOLIBRI-KID/ADO instrument shows at least satisfactory psychometric properties according to the results of both classical test theoretical and IRT analyses. Further evidence of its applicability should be explored in the ongoing validation study by performing multidimensional IRT analyses.
Water-Induced Changes in Experimental Resin Composites Functionalized with Conventional (45S5) and Customized Bioactive Glass
Authors: Muradbegovic Alen , Par Matej , Panduric Vlatko , Zugec Paula , Tauböck Tobias T. , Attin Thomas , Tarle Zrinka , Marovic Danijela
Published: May 2023

The aim of the study was to evaluate microhardness, mass changes during 1-year water immersion, water sorption/solubility, and calcium phosphate precipitation of experimental composites functionalized with 5–40 wt% of two types of bioactive glass (BG): 45S5 or a customized low-sodium fluoride-containing formulation. Vickers microhardness was evaluated after simulated aging (water storage and thermocycling), water sorption and solubility were tested according to ISO 4049, and calcium phosphate precipitation was studied by scanning electron microscopy, energy dispersive X-ray spectroscopy, and Fourier-transform infrared spectroscopy. For the composites containing BG 45S5, a significant reduction in microhardness was observed with increasing BG amount. In contrast, 5 wt% of the customized BG resulted in statistically similar microhardness to the control material, while higher BG amounts (20 and 40 wt%) resulted in a significant improvement in microhardness. Water sorption was more pronounced for composites containing BG 45S5, increasing 7-fold compared to the control material, while the corresponding increase for the customized BG was only 2-fold. Solubility increased with higher amounts of BG, with an abrupt increase at 20 and 40 wt% of BG 45S5. Calcium phosphate was precipitated by all composites with BG amounts of 10 wt% or more. The improved properties of the composites functionalized with the customized BG indicate better mechanical, chemical, and dimensional stability without compromising the potential for calcium phosphate precipitation.
The Reliability of the Wall Drop Punt Kick and Catch Test
Authors: Matos Rui , Amaro Nuno , Lopes Nataniel , Costa Pedro , Jacinto Miguel , Rodrigues Filipe , Antunes Raul , Coelho Luís , Ibáñez Sergio , Monteiro Diogo
Published: May 2023

This study aimed to evaluate the reliability of a manipulative eye–segmental (hand and foot) coordination task, namely the Wall Drop Punt Kick and Catch test (WDPK&C), over two weeks. Forty-one children and adolescents (18 boys, 23 girls) with a mean age of 10.2 (SD = 1.62) years old were recruited for assessment. Subjects had 30 s to perform as many ball impacts as possible on a wall two meters away, following a drop punt kick, rebound on the wall, and catch sequence. The Intraclass Correlation Coefficient (ICC = 0.896) for unique measures, Cronbach Alpha (α = 0.945), and Lin’s Concordance Correlation Coefficient (CCC = 0.896) provide evidence of reliability considering two consecutive measurements. These results further support the reliability of the WDPK&C test in a sample of Portuguese children and adolescents. Thus, the WDPK&C test can be applied to Portuguese boy and girl children and adolescents. Forthcoming studies should test the reliability of this test across different age groups since it is intended to be a test with a wide lifespan coverage.
Exercise Prescription for the Work–Life Population and Beyond
Authors: Sjøgaard Gisela , Søgaard Karen , Hansen Anne Faber , Østergaard Anne Skov , Teljigovic Sanel , Dalager Tina
Published: May 2023

The background for this paper concerns a high frequency of work-related disorders that may result from physical exposure at work being highly sedentary, repetitive–monotonous, or physically demanding. This may result in levels of physical inactivity or strenuous activity impairing health. The aim is to present an evidence-based exercise prescription for the work–life population and beyond. The exercise program is designed to be feasible for use at the workplace and/or during leisure time and to improve health, workability, productivity, sickness absence, etc. The specific concept of Intelligent Physical Exercise Training, IPET, includes the assessment of several health-related variables, including musculoskeletal disorders, physical capacity, and physical exposure at work and/or daily life activity. An algorithm with cut-points for prescribing specific exercises is provided. Exercise programs in praxis are addressed through descriptions of precise executions of various prescribed exercises and possible alternatives to optimize variation and adherence. Finally, perspectives on the significance of introducing IPET and the ongoing, as well as future lines of development, are discussed.
Comprehensive Analysis of the Impact of the Icing of Wind Turbine Blades on Power Loss in Cold Regions
Authors: Chuang Zhenju , Yi Hui , Chang Xin , Liu Hongxiang , Zhang Haidian , Xia Lulin
Published: May 2023

Blade icing often occurs on wind turbines in cold climates. Blade icing has many adverse effects on wind turbines, and the loss of output power is one of the most important effects. With the increasing emphasis on clean energy around the world, the design and production of wind turbines tend to be large-scale. So this paper selected the 15 MW wind turbine provided by NREL (American Renewable Energy Laboratory) to study the influence of blade icing on output power. In this paper, a multi-program coupled analysis method named CFD-WTIC-ILM (CFD: Computational fluid dynamics; WTIC: Wind Turbine Integrated Calculation; ILM: Ice loss method) was proposed to analyze the whole machine wind turbine. Firstly, Fensap-ice was used to simulate the icing of the wind turbine blades, and then the icing results were input into WTIC for the integrated calculation and analysis of the wind turbine. Then, the WTIC calculation results were used to simulate SCADA (supervisory control and data acquisition) data and input into ILM to calculate the power loss. Finally, this paper analyzed the comprehensive influence of icing on output power. The calculation results show that the ice mainly accumulates on the windward side of the blade. Icing has a great influence on the aerodynamic characteristics of the airfoil, leading to a significant decrease in the power curve. The rated wind speed is pushed from 10.59 m/s to 13 m/s. The power loss of the wind turbine in the wind speed optimization stage is as high as 37.48%, and the annual power loss rate caused by icing can reach at least 22%.
Research on Wind Turbine Blade Damage Fault Diagnosis Based on GH Bladed
Authors: Xing Zhitai , Jia Yan , Zhang Lei , Song Xiaowen , Zhang Yanfeng , Wu Jianxin , Wang Zekun , Guo Jicai , Li Qingan
Published: May 2023

With the increasing installed capacity of wind turbines, ensuring the safe operation of wind turbines is of great significance. However, the failure of wind turbines is still a severe problem, especially as blade damage can cause serious harm. To detect blade damage in time and prevent the accumulation of microdamage of blades evolving into severe injury, a damage dataset based on GH Bladed simulation of blade damage is proposed. Then, based on the wavelet packet analysis theory method, the MATLAB software can automatically analyze and extract the energy characteristics of the signal to identify the damage. Finally, the GH Bladed simulation software and MATLAB software are combined for fault diagnosis analysis. The results show that the proposed method based on GH Bladed to simulate blade damage and wavelet packet analysis can extract damage characteristics and identify single-unit damage, multiple-unit damage, and different degrees of damage. This method can quickly and effectively judge the damage to wind turbine blades; it provides a basis for further research on wind turbine blade damage fault diagnosis.
A Hybrid Excitation Model Based Lightweight Siamese Network for Underwater Vehicle Object Tracking Missions
Authors: Wu Xiaofeng , Han Xinyue , Zhang Zongyu , Wu Han , Yang Xu , Huang Hai
Published: May 2023

Performing object tracking tasks and efficiently perceiving the underwater environment in real time for underwater vehicles is a challenging task due to the complex nature of the underwater environment. A hybrid excitation model based lightweight Siamese network is proposed to solve the mismatch between underwater objects with limited characteristics and complex deep learning models. The lightweight neural network is applied to the residual network in the Siamese network to reduce the computational complexity and cost of the model while constructing a deeper network. In addition, to deal with the changeable complex underwater environment and consider the timing of video tracking, the global excitation model (HE module) is introduced. The model adopts the excitation methods of space, channel, and motion to improve the accuracy of the algorithm. Based on the designed underwater vehicle, the underwater target tracking and target grabbing experiments are carried out, and the experimental results show that the proposed tracking algorithm has a high tracking success rate.
Characteristics of Coastal Low-Level Jets in the Boundary Layer of the Pearl River Estuary
Authors: Qiu Zongxu , Xian Jinhong , Yang Yuexin , Lu Chao , Yang Honglong , Hu Yuanyuan , Sun Jiaqi , Zhang Chunsheng
Published: May 2023

It is well-known that coastal low-level jets (CLLJs) play an essential role in transporting heat, water vapor and pollutants. However, the CLLJ characteristics in the Pearl River Estuary have not been deeply revealed due to the lack of long-term observations. Based on the long-term observations from a wind lidar, we analyze the primary climatic characteristics of the CLLJs in the Pearl River Estuary and investigate their relationships with large-scale and local-scale synoptic systems. The results show that the CLLJs mainly appear during the flood season, with the most occurrence in May. The CLLJ occurrence during the flood season is mainly influenced by the large-scale north–south pressure gradient driven by the western Pacific subtropical high and terrestrial low-pressure systems. The occurrence of the CLLJs exhibits a distinct diurnal cycle with two different peaks in different seasons. One peak appears at nighttime, mainly during non-flood seasons. The other appears in the afternoon, mainly during the flood season. In the non-flood seasons, under the influence of cold air, the inertial oscillations triggered by the land–sea thermal contrast lead to CLLJ onset at nighttime in the Pearl River Estuary. During the flood season, the strong near-surface pressure gradient contributes to CLLJ onset in the afternoon, while the topography (blocking and passing) is more conducive to the occurrence of the CLLJs in the Pearl River Estuary. These findings reveal the formation mechanisms of the CLLJs over the Pearl River Estuary, thus providing a basis for further understanding the precipitation in the Pearl River Estuary and the occurrence of the CLLJs in other coastal areas with complex mountain ranges.
Research on a Horizon Line Detection Method for Unmanned Surface Vehicles in Complex Environments
Authors: Shi Binghua , Wang Chen , Di Yi , Guo Jia , Zhang Ziteng , Long Yang
Published: May 2023

A critical step in the visual navigation of unmanned surface vehicles (USVs) is horizon line detection, which can be used to adjust the altitude as well as for obstacle avoidance in complex environments. In this paper, a real-time and accurate detection method for the horizon line is proposed. Our approach first differentiates the complexity of navigational scenes using the angular second moment (ASM) parameters in the grey level co-occurrence matrix (GLCM). Then, the region of interest (ROI) is initially extracted using minimal human interaction for the complex navigation scenes, while subsequent frames are dynamically acquired using automatic feature point matching. The matched ROI can be maximally removed from the complex background, and the Zernike-moment-based edges are extracted from the obtained ROI. Finally, complete sea horizon information is obtained through a linear fitting of the lower edge points to the edge information. Through various experiments carried out on a classical dataset, our own datasets, and that of another previously published paper, we illustrate the significance and accuracy of this technique for various complex environments. The results show that the performance has potential applications for the autonomous navigation and control of USVs.
Changes in the Available Potential and Kinetic Energy of Mesoscale Vortices When They Are Stretched into Filaments
Authors: Zhmur Vladimir V. , Belonenko Tatyana V. , Travkin Vladimir S. , Novoselova Elena V. , Harutyunyan David A. , Raj Roshin P.
Published: May 2023

The article discusses various aspects of the interaction of vortices with the barotropic flow. Vortex interaction with a flow results in rotation variants, nutational oscillations, and unlimited stretching of its core. The vortex remains in a localized formation, with the semi-axes of the ellipse experiencing fluctuations near an average value in the first two cases. In the third case, the vortex is significantly elongated, and its shape in the horizontal plane changes as follows: one axis of the ellipse increases, and the other decreases. In this case, the vortex, when viewed from above, stretches into a thread, while remaining ellipsoidal. These vortex formations are called filaments. The latter arise from initially almost circular vortices in the horizontal plane and represent structures with non-zero vorticity elongated in one direction. Here, we aim to study the energy transformation of a vortex during its evolution process, mainly due to changes in its shape by stretching. The energy evolution of a mesoscale vortex located in the Norwegian Sea is analyzed using GLORYS12V1 ocean reanalysis data to verify the theoretical conclusions. During the evolution, the vortex is found to transform from a round shape and becomes elongated, and after three weeks its longitudinal scale becomes 4 times larger than the transverse one. During the transformation of a vortex, the kinetic energy and available potential energy decrease respectively by 3 times and 1.7 times. Concurrently, the total energy of the vortex is found to decrease by 2.3 times. We argue that the stretching of vortices results in a loss of energy as well as its redistribution from mesoscale to submesoscale. The lost part of the energy returns to the flow and results in the occurrence of the reverse energy cascade phenomenon.
Diversity and Antimicrobial Activity of Intestinal Fungi from Three Species of Coral Reef Fish
Authors: Liao Xinyu , Yang Jiadenghui , Zhou Zanhu , Wu Jinying , Xu Dunming , Yang Qiaoting , Zhong Saiyi , Zhang Xiaoyong
Published: May 2023

Although intestinal microbiota play crucial roles in fish digestion and health, little is known about intestinal fungi in fish. This study investigated the intestinal fungal diversity of three coral reef fish (Lates calcarifer, Trachinotus blochii, and Lutjanus argentimaculatus) from the South China Sea using a culturable method. A total of 387 isolates were recovered and identified by sequencing their internal transcribed spacer sequences, belonging to 29 known fungal species. The similarity of fungal communities in the intestines of the three fish verified that the fungal colonization might be influenced by their surrounding environments. Furthermore, the fungal communities in different intestines of some fish were significantly different, and the number of yeasts in the hindgut was less than that in fore- and mid-intestines, suggesting that the distribution of fungi in fishes’ intestines may be related to the physiological functions of various intestinal segments. In addition, 51.4% of tested fungal isolates exhibited antimicrobial activity against at least one marine pathogenic microorganism. Notably, isolate Aureobasidium pullulans SCAU243 exhibited strong antifungal activity against Aspergillus versicolor, and isolate Schizophyllum commune SCAU255 displayed extensive antimicrobial activity against four marine pathogenic microorganisms. This study contributed to our understanding of intestinal fungi in coral reef fish and further increased the library of fungi available for natural bioactive product screening.
Entomopathogenicity of Ascomycete Fungus Cordyceps militaris on the Cotton Bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae)
Authors: Glover James P. , Nufer Marissa I. , Perera Omaththage P. , Portilla Maribel , George Justin
Published: May 2023

This study investigated the exposure of the cotton bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) to a novel pathogenic fungal agent historically associated with human medicinal value, a commercial strain of Cordyceps militaris ((L.) Fr.) Vuill. (Hypocreales). A series of comparative studies were conducted to evaluate the efficacy of two different exposure methods using four concentrations (n × 109, n × 108, n × 107, n × 106) of C. militaris, where n × 109 provided a concentration of approximately 420 ± 37 spores per mm2 with 398 ± 28 viable spores. Survival of cotton bollworms of all stages was not affected by C. militaris at any concentration 1 d post-exposure. The greatest reduction in survival and highest sporulation rates were observed primarily on or after 7 d post-exposure for early instars (first and second). Significant declines in the survival of early instars were observed for all concentrations at 7 d, and 95% mortality by 10 d, with the exception of the fifth instars that experienced a less severe reduction in survival (35%) when exposed to any concentrations used in the study. Survival of late instars (third to fifth) ranged from 44% to 68% on day 10, while adult survival was near 99% across the duration of the experiment. The relatively narrow range observed for both the lethal concentration and sporulation of second, third, and fifth instar cotton bollworms exposed to the C. militaris strain may demonstrate potential field application for control of larval populations of cotton bollworms.