bluuebunny/crossref_metadata_2025 huggingface dataset:
Expression of Autophagy-Related Factors LC3A and Beclin 1 and Apoptosis-Related Factors Bcl-2 and BAX in Osteoblasts Treated With Sodium Fluoride
Authors: Xu Lin , Deng Chaonan , Zhang Ying , Zhao Lina , Linghu Yan , Yu Yanni
Published: July 2021

Objective

This study aims to analyze the expressions of autophagy-related factors light chain 3 alpha (LC3A) and Beclin 1 and apoptosis-related factors B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (BAX) in primary osteoblasts treated with sodium fluoride (NaF).

Methods

Osteoblasts were extracted from Sprague-Dawley rats and treated with 0, 2.5, 5, and 10 mg/L NaF solutions, followed by 10 mmol/L 3-methyladenine (3-MA) for 24 h. The apoptotic rate was determined by flow cytometry, and the expressions of the autophagy- and apoptosis-related factors were measured by western blotting and real-time quantitative polymerase chain reaction.

Results

The mRNA expressions of LC3A, Beclin 1, and BAX in the NaF-treated osteoblast group were higher than those in the control group, while the protein expressions of these factors in the NaF-treated group were significantly higher than those in the control group. However, the Bcl-2 protein expression in the NaF-treated osteoblasts was significantly decreased compared to that in the control cells. After the 3-MA treatment, the protein expressions of LC3A, Beclin 1, and Bcl-2 were significantly decreased compared with those of the NaF-treated group, whereas the expression of BAX increased. Moreover, the apoptosis rate was increased after the addition of the 3-MA inhibitor.

Conclusion

NaF stimulation promoted autophagy and apoptosis of the osteoblasts, suggesting the involvement of fluoride damage in these processes.
Physiological Responses During High-Intensity Interval Training in Young Swimmers
Authors: Almeida Tiago André Freire , Pessôa Filho Dalton Müller , Espada Mário Cunha , Reis Joana Filipa , Sancassani Andrei , Massini Danilo Alexandre , Santos Fernando Jorge , Alves Francisco Besone
Published: July 2021

This study analyzed whether 100- and 200-m interval training (IT) in swimming differed regarding temporal, perceptual, and physiological responses. The IT was performed at maximal aerobic velocity (MAV) until exhaustion and time spent near to maximalVO2 peak oxygen uptake (⩒O2peak), total time limit (tLim), peak blood lactate [La−] peak, ⩒O2 kinetics (⩒O2K), and rate of perceived exertion (RPE) were compared between protocols. Twelve swimmers (seven males 16.1 ± 1.1 and five females 14.2 ± 1 years) completed a discontinuous incremental step test for the second ventilatory threshold (VT2), ⩒O2peak, and MAV assessment. The swimmers subsequently completed two IT protocols at MAV with 100- and 200-m bouts to determine the maximal ⩒O2 (peak-⩒O2) and time spent ≥VT2, 90, and 95% of ⩒O2peak for the entire protocols (IT100 and IT200) and during the first 800-m of each protocol (IT8x100 and IT4x200). A portable apparatus (K4b2) sampled gas exchange through a snorkel and an underwater led signal controlled the velocity. RPE was also recorded. The Peak-⩒O2 attained during IT8x100 and IT4x200 (57.3 ± 4.9 vs. 57.2 ± 4.6 ml·kg−1·min−1) were not different between protocols (p = 0.98) nor to ⩒O2peak (59.2 ± 4.2 ml·kg−1·min−1, p = 0.37). The time constant of ⩒O2K (24.9 ± 8.4 vs. 25.1 ± 6.3-s, p = 0.67) and [La−] peak (7.9 ± 3.4 and 8.7 ± 1.5 mmol·L−1, p = 0.15) also did not differ between IT100 and IT200. The time spent ≥VT2, 90, and 95%⩒O2peak were also not different between IT8x100 and IT4x200 (p = 0.93, 0.63, and 1.00, respectively). The RPE for IT8x100 was lower than that for IT4x200 (7.62 ± 2 vs. 9.5 ± 0.7, p = 0.01). Both protocols are considered suitable for aerobic power enhancement, since ⩒O2peak was attained with similar ⩒O2K and sustained with no differences in tLim. However, the fact that only the RPE differed between the IT protocols suggested that coaches should consider that nx100-m/15-s is perceived as less difficult to perform compared with nx200-m/30-s for the first 800-m when managing the best strategy to be implemented for aerobic power training.
Long Chain Fatty Acids as Modulators of Immune Cells Function: Contribution of FFA1 and FFA4 Receptors
Authors: Hidalgo Maria A. , Carretta Maria D. , Burgos Rafael A.
Published: July 2021

Long-chain fatty acids are molecules that act as metabolic intermediates and constituents of membranes; however, their novel role as signaling molecules in immune function has also been demonstrated. The presence of free fatty acid (FFA) receptors on immune cells has contributed to the understanding of this new role of long-chain fatty acids (LCFAs) in immune function, showing their role as anti-inflammatory or pro-inflammatory molecules and elucidating their intracellular mechanisms. The FFA1 and FFA4 receptors, also known as GPR40 and GPR120, respectively, have been described in macrophages and neutrophils, two key cells mediating innate immune response. Ligands of the FFA1 and FFA4 receptors induce the release of a myriad of cytokines through well-defined intracellular signaling pathways. In this review, we discuss the cellular responses and intracellular mechanisms activated by LCFAs, such as oleic acid, linoleic acid, palmitic acid, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), in T-cells, macrophages, and neutrophils, as well as the role of the FFA1 and FFA4 receptors in immune cells.
Male Age Influences Re-mating Incidence and Sperm Use in Females of the Dengue Vector Aedes aegypti
Authors: Agudelo Juliana , Alfonso-Parra Catalina , Avila Frank W.
Published: July 2021

Diseases transmitted by female Aedes aegypti mosquitoes are public health issues in countries in the tropics and sub-tropics. As in other insects, A. aegypti females undergo behavioral and physiological changes upon mating that principally act to facilitate the production of progeny. The primary effectors of A. aegypti female post-mating responses are male-derived seminal proteins that are transferred to females during mating. Increased male age reduces ejaculate function in numerous taxa and alters seminal protein composition in Drosophila melanogaster, but the impacts of male age on female A. aegypti post-mating responses are unknown. Here, we used “old” (21–22 days old) and “young” (4–5 days old) A. aegypti males to assess the influence of male age on oviposition, fertility, and re-mating incidence in their mates. We also examined how age influenced paternity share in females initially mated to young or old males that subsequently re-mated with a transgenic male that transferred RFP-labeled sperm and whose progeny inherited a larval-expressed GFP marker. We found that increased male age had no effect on female fecundity or fertility but significantly impacted their ability to prevent re-mating in their mates—more than half (54.5%) of the females mated to an old male re-mated, compared to 24% of females initially mated to a young male. Polyandrous A. aegypti females displayed first male precedence regardless of the age of their initial mate. However, young males were better able to compete with rival male sperm, siring significantly more progeny (77%) compared to old males (64%). Young males had significantly more sperm in their seminal vesicles than old males at the time of mating, although males of both age groups transferred similar numbers of sperm to their mates. Our results suggest that male senescence differentially impacts the induction of some post-mating changes in A. aegypti females. As the effect of age may be further exacerbated in the field, age-related declines in male ability to induce sexual refractoriness have implications for A. aegypti population control programs that release adults into the environment.
Protein Composition of Circulating Extracellular Vesicles Immediately Changed by Particular Short Time of High-Intensity Interval Training Exercise
Authors: Kobayashi Yoshinao , Eguchi Akiko , Tamai Yasuyuki , Fukuda Sanae , Tempaku Mina , Izuoka Kiyora , Iwasa Motoh , Takei Yoshiyuki , Togashi Kenji
Published: July 2021

Introduction/Purpose

High-intensity interval training (HIIT) promotes various biological processes and metabolic effects in multiple organs, but the role of extracellular vesicles (EVs) released from a variety of cells is not fully understood during HIIT exercise (HIIT-Ex). We investigated the changes in circulating number and proteomic profile of EVs to assess the effect of HIIT-Ex.

Methods

Seventeen young men (median age, 20 years) were enrolled in the study. Total duration of the HIIT-Ex was 4 min. Blood samples were collected from before HIIT-Ex (pre-HIIT-Ex), at the immediate conclusion of HIIT-Ex (T0), at 30 min (T30), and at 120 min after HIIT-Ex. The pulse rate and systolic blood pressure were measured. Circulating EVs were characterized, and EV proteins were detected via nano liquid chromatography tandem mass spectrometry.

Results

The pulse rate and systolic blood pressure at T0 to pre-HIIT-Ex were significantly higher. Circulating EV number was significantly altered throughout the HIIT-Ex, and the source of circulating EVs included skeletal muscle, hepatocytes, and adipose tissue. Proteomic analysis identified a total of 558 proteins within isolated circulating EVs from pre-HIIT-Ex, T0, and T30. Twenty proteins in total were significantly changed at pre-HIIT-Ex, T0, and T30 and are involved in a variety of pathways, such as activation of coagulation cascades, cellular oxidant detoxification, and correction of acid–base imbalance. Catalase and peroxiredoxin II were increased at T0.

Conclusion

The circulating EV composition can be immediately changed by particularly a short time of HIIT-Ex, indicating that EVs may intercommunicate across various organs rapidly in response to HIIT-Ex.
Level, Uphill, and Downhill Running Economy Values Are Correlated Except on Steep Slopes
Authors: Lemire Marcel , Falbriard Mathieu , Aminian Kamiar , Millet Grégoire P. , Meyer Frédéric
Published: July 2021

The aim of this study was first to determine if level, uphill, and downhill energy cost of running (ECR) values were correlated at different slopes and for different running speeds, and second, to determine the influence of lower limb strength on ECR. Twenty-nine healthy subjects completed a randomized series of 4-min running bouts on an instrumented treadmill to determine their cardiorespiratory and mechanical (i.e., ground reaction forces) responses at different constant speeds (8, 10, 12, and 14 km·h−1) and different slopes (−20, −10, −5, 0, +5, +10, +15, and +20%). The subjects also performed a knee extensor (KE) strength assessment. Oxygen and energy costs of running values were correlated between all slopes by pooling all running speeds (all r2 ≥ 0.27; p ≤ 0.021), except between the steepest uphill vs. level and the steepest downhill slope (i.e., +20% vs. 0% and −20% slopes; both p ≥ 0.214). When pooled across all running speeds, the ECR was inversely correlated with KE isometric maximal torque for the level and downhill running conditions (all r2 ≥ 0.24; p ≤ 0.049) except for the steepest downhill slope (−20%), but not for any uphill slopes. The optimal downhill grade (i.e., lowest oxygen cost) varied between running speeds and ranged from −14% and −20% (all p < 0.001). The present results suggest that compared to level and shallow slopes, on steep slopes ~±20%, running energetics are determined by different factors (i.e., reduced bouncing mechanism, greater muscle strength for negative slopes, and cardiopulmonary fitness for positive slopes). On shallow negative slopes and during level running, ECR is related to KE strength.
Phenolics Profile and Antioxidant Activity Analysis of Kiwi Berry (Actinidia arguta) Flesh and Peel Extracts From Four Regions in China
Authors: Zhang Jiyue , Gao Ningxuan , Shu Chi , Cheng Shunchang , Sun Xiyun , Liu Changjiang , Xin Guang , Li Bin , Tian Jinlong
Published: July 2021

The kiwi berry (Actinidia arguta) has been widely studied because of its rich phenolic, flavonoid, and vitamin C contents. Numerous reports have demonstrated that fruit peels contain higher phenolic content and antioxidant activity than that of flesh. In this study, the phytochemical content and antioxidant activities of peel and flesh extracts of six kiwi berries were analyzed from four regions (namely, Dandong, Benxi, Taian, and Tonghua) in China. The antioxidant activity was determined using the peroxyl radical scavenging capacity (PSC) and cellular antioxidant activity (CAA) assays. The phenolic, flavonoid, and vitamin C contents of kiwi berry peel were 10.77, 13.09, and 10.38 times richer than that of kiwi berry flesh, respectively. In addition, the PSC and CAA values of kiwi berry peel were higher than those of kiwi berry flesh. The analysis of the separation and contents of phenolics were performed by the high-performance liquid chromatography (HPLC)-diode-array detectormass spectrometry/mass (DAD-MS/MS) system, and the results illustrated that protocatechuic acid, caffeic acid, chlorogenic acid, and quinic acid were the major phenolic compounds. In conclusion, this study indicated that kiwi berry peel contains a rich source of antioxidants. These data are of great significance for the full development and utilization of kiwi berries in these four regions of China to produce nutraceutical and functional foods.
Sap Flow Disruption in Grapevine Is the Early Signal Predicting the Structural, Functional, and Genetic Responses to Esca Disease
Authors: Ouadi Loris , Bruez Emilie , Bastien Sylvie , Yacoub Amira , Coppin Cindy , Guérin-Dubrana Lucia , Fontaine Florence , Domec Jean-Christophe , Rey Patrice
Published: July 2021

Fungal species involved in Esca cause the formation of grapevine wood necroses. It results in the deterioration of vascular network transport capacity and the disturbance of the physiological processes, leading to gradual or sudden grapevine death. Herein, for two consecutive growing seasons, a detailed analysis of the structural (wood necrosis and leaf discoloration) and physiological parameters related to the water use of healthy and esca-symptomatic grapevines was conducted. Measurements were carried out on 17-year-old grapevines that expressed, or not, Esca-leaf symptoms in a vineyard of the Bordeaux region (France). Whole-plant transpiration was recorded continuously from pre-veraison to harvest, using noninvasive sap flow sensors. Whole-plant transpiration was systematically about 40–50% lower in Esca-diseased grapevines compared with controls, and this difference can be observed around 2 weeks before the first Esca-foliar symptoms appeared in the vineyard. Unlike grapevine sap flow disruption, structural (e.g., leaf discolorations), functional (e.g., stomatal conductance, photosynthetic activity, phenolic compounds), and genetic (e.g., expression of leaf-targeted genes) plant responses were only significantly impacted by Esca at the onset and during leaf symptoms development. We conclude that sap flow dynamic, which was related to a high level of a white-rot necrosis, provides a useful tool to predict plant disorders due to Esca-grapevine disease.
Identification of Chemotypic Markers in Three Chemotype Categories of Cannabis Using Secondary Metabolites Profiled in Inflorescences, Leaves, Stem Bark, and Roots
Authors: Jin Dan , Henry Philippe , Shan Jacqueline , Chen Jie
Published: July 2021

Previous chemotaxonomic studies of cannabis only focused on tetrahydrocannabinol (THC) dominant strains while excluded the cannabidiol (CBD) dominant strains and intermediate strains (THC ≈ CBD). This study investigated the utility of the full spectrum of secondary metabolites in different plant parts in three cannabis chemotypes (THC dominant, intermediate, and CBD dominant) for chemotaxonomic discrimination. Hierarchical clustering, principal component analysis (PCA), and canonical correlation analysis assigned 21 cannabis varieties into three chemotypes using the content and ratio of cannabinoids, terpenoids, flavonoids, sterols, and triterpenoids across inflorescences, leaves, stem bark, and roots. The same clustering results were obtained using secondary metabolites, omitting THC and CBD. Significant chemical differences were identified in these three chemotypes. Cannabinoids, terpenoids, flavonoids had differentiation power while sterols and triterpenoids had none. CBD dominant strains had higher amounts of total CBD, cannabidivarin (CBDV), cannabichromene (CBC), α-pinene, β-myrcene, (−)-guaiol, β-eudesmol, α-eudesmol, α-bisabolol, orientin, vitexin, and isovitexin, while THC dominant strains had higher total THC, total tetrahydrocannabivarin (THCV), total cannabigerol (CBG), camphene, limonene, ocimene, sabinene hydrate, terpinolene, linalool, fenchol, α-terpineol, β-caryophyllene, trans-β-farnesene, α-humulene, trans-nerolidol, quercetin, and kaempferol. Compound levels in intermediate strains were generally equal to or in between those in CBD dominant and THC dominant strains. Overall, with higher amounts of β-myrcene, (−)-guaiol, β-eudesmol, α-eudesmol, and α-bisabolol, intermediate strains more resemble CBD dominant strains than THC dominant strains. The results of this study provide a comprehensive profile of bioactive compounds in three chemotypes for medical purposes. The simultaneous presence of a predominant number of identified chemotype markers (with or without THC and CBD) could be used as chemical fingerprints for quality standardization or strain identification for research, clinical studies, and cannabis product manufacturing.
Assessing Instructional Cognitive Load in the Context of Students' Psychological Challenge and Threat Orientations: A Multi-Level Latent Profile Analysis of Students and Classrooms
Authors: Martin Andrew J. , Ginns Paul , Burns Emma C. , Kennett Roger , Munro-Smith Vera , Collie Rebecca J. , Pearson Joel
Published: July 2021

To better understand instructional cognitive load, it is important to operationalize and assess it in novel ways that can reveal how different students perceive and experience this load as either challenging or threatening. The present study administered a recently developed instruction assessment tool—the Load Reduction Instruction Scale-Short (LRIS-S)—toN= 2,071 students in 188 high school science classrooms. Multilevel latent profile analysis (LPA) was used to identify student and classroom profiles based on students' reports of instructional cognitive load (load reduction instruction, LRI; using the LRIS-S) and their accompanying psychological challenge orientations (self-efficacy and growth goals), and psychological threat orientations (anxiety and failure avoidance goals). In phase 1 of analyses (investigating students; Level 1), we identified 5 instructional-psychological student profiles that represented different presentations of instructional load, challenge orientation, and threat orientation, ranging from the most maladaptive profile (the Instructionally-Overburdened & Psychologically-Resigned profile) to the most adaptive profile (Instructionally-Optimized & Psychologically-Self-Assured profile). The derived profiles revealed that similar levels of perceived instructional load can be accompanied by different levels of perceived challenge and threat. For example, we identified two profiles that were both instructionally-supported but who varied in their accompanying psychological orientations. Findings also identified profiles where students were dually motivated by both challenge and threat. In turn, these profiles (and their component scores) were validated through their significant associations with persistence, disengagement, and achievement. In phase 2 of analyses (investigating students and classrooms; Levels 1 and 2), we identified 3 instructional-psychological classroom profiles that varied in instructional cognitive load, challenge orientations, and threat orientations: Striving classrooms, Thriving classrooms, and Struggling classrooms. These three classroom profiles (and their component scores) were also validated through their significant associations with classroom-average persistence, disengagement, and achievement—with Struggling classrooms reflecting the most maladaptive outcomes and Thriving classrooms reflecting the most adaptive outcomes. Taken together, findings show that considering instructional cognitive load (and new approaches to empirically assessing it) in the context of students' accompanying psychological orientations can reveal unique insights about students' learning experiences and about important differences between classrooms in terms of the instructional load that is present.
Associations Between Defence-Style, Eating Disorder Symptoms, and Quality of Life in Community Sample of Women: A Longitudinal Exploratory Study
Authors: Aouad Phillip , Hay Phillipa , Foroughi Nasim , Cosh Suzanne M. , Mannan Haider
Published: July 2021

Background and Aim: Eating Disorders (EDs) impact an estimated 15% of the global population and are linked to maladaptive defence-styles (coping strategies) and poorer mental health outcomes. Defence-styles have been grouped into immature, neurotic, and mature behaviours. Studies have yet to examine all three defence-styles in ED symptomatic individuals over an extended period of time. The current study aimed to investigate using converse analysis the relationships between defence-style and ED outcomes over a 5-years period.

Methods: Participants (n = 216, mean age 33 years) were recruited through the Women's Eating and Health Literacy study, with the current study examining a 5-years period of two waves (year-4 and year-9). The current study tested associations over time between eating pathology (EDE-Q), psychological distress (K10), mental and physical health related quality of life (M/PHRQoL, SF-12), and defence-style (DSQ-40).

Results: Mature, immature and neurotic defence-styles did not significantly change over 5 years. Over the same period, only PHRQoL significantly predicted mature defence-styles having positive effect. Both MHRQoL and PHRQoL significantly predicted immature defence-styles having positive and negative effects, respectively. Psychological distress, PHRQoL and weight concern significantly predicted neurotic defence-styles having positive effects except for psychological distress. PHRQoL, MHRQoL, restraint and eating concern significantly predicted overall eating pathology having positive effects except for PHRQoL and MHRQoL. Conversely, among the defence-style variables, over 5 years, both immature and neurotic defence-styles significantly predicted psychological distress having positive effects, immature and mature defence-styles significantly predicted MHRQoL having negative and positive effects, respectively, while only immature defence-styles significantly predicted overall eating pathology having positive effect.

Conclusions: The results of the current study suggest that immaturity and neuroticism but not maturity were the defence-style variables predicting psychological distress over a 5-years period while conversely psychological distress predicted only neurotic defence styles. The findings of the current study may suggest that without intervention, mature, immature and neurotic defence-styles may largely remain immutable to significant shifts over time. Limitations in the current study included limited demographic representation. The current study is anticipated to generate considerations into treatments that could strengthen defence-styles in individuals with increased eating pathology.
Measuring Creative Self-Efficacy: An Item Response Theory Analysis of the Creative Self-Efficacy Scale
Authors: Shaw Amy , Kapnek Melissa , Morelli Neil A.
Published: July 2021

Applying the graded response model within the item response theory framework, the present study analyzes the psychometric properties of Karwowski’s creative self-efficacy (CSE) scale. With an ethnically diverse sample of US college students, the results suggested that the six items of the CSE scale were well fitted to a latent unidimensional structure. The scale also had adequate measurement precision or reliability, high levels of item discrimination, and an appropriate range of item difficulty. Gender-based differential item functioning analyses confirmed that there were no differences in the measurement results of the scale concerning gender. Additionally, openness to experience was found to be positively related to the CSE scale scores, providing some support for the scale’s convergent validity. Collectively, these results confirmed the psychometric soundness of the CSE scale for measuring CSE and also identified avenues for future research.
Does Co-worker Presenteeism Increase Innovative Behavior? Evidence From IT Professionals Under the 996 Work Regime in China
Authors: Yang Tianan , Liu Ran , Deng Jianwei
Published: July 2021

Drawing on the event system and regulatory focus theory, this study constructed an impact mechanism model to investigate the relationship between the event strength of co-worker presenteeism and innovative behavior among IT professionals under the 996 work regime. In addition to test the direct effect, we examined the indirect effect of promotion focus and the moderating effect of event time in this relationship. Data were collected through an online survey administered to 374 IT professionals in China. The results showed a positive relationship between the criticality of co-worker presenteeism events and innovative behavior. An indirect effect of promotion focus was also found in this relationship. The timing of co-worker presenteeism events moderated the relationship between the criticality of co-worker presenteeism events and promotion focus. Specifically, the effect was more significant when co-worker presenteeism events occurred during project delays.
Adequate Sample Sizes for a Three-Level Growth Model
Authors: Lee Eunsoo , Hong Sehee
Published: July 2021

Multilevel models have been developed for addressing data that come from a hierarchical structure. In particular, due to the increase of longitudinal studies, a three-level growth model is frequently used to measure the change of individuals who are nested in groups. In multilevel modeling, sufficient sample sizes are needed to obtain unbiased estimates and enough power to detect individual or group effects. However, there are few sample size guidelines for three-level growth models. Therefore, it is important that researchers recognize the possibility of unreliable results when sample sizes are small. The purpose of this study is to find adequate sample sizes for a three-level growth model under realistic conditions. A Monte Carlo simulation was performed under 12 conditions: (1) level-2 sample size (10, 30), (2) level-3 sample size (30, 50, 100) (3) intraclass correlation at level-3 (0.05, 0.15). The study examined the following outcomes: convergence rate, relative parameter bias, mean square error (MSE), 95% coverage rate and power. The results indicate that estimates of the regression coefficients are unbiased, but the variance component tends to be inaccurate with small sample sizes.
The Development of Categorical Perception of Segments and Suprasegments in Mandarin-Speaking Preschoolers
Authors: Ma Junzhou , Zhu Jiaqiang , Yang Yuxiao , Chen Fei
Published: July 2021

This study investigated the developmental trajectories of categorical perception (CP) of segments (i.e., stops) and suprasegments (i.e., lexical tones) in an attempt to examine the perceptual development of phonological categories and whether CP of suprasegments develops in parallel with that of segments. Forty-seven Mandarin-speaking monolingual preschoolers aged four to six years old, and fourteen adults completed both identification and discrimination tasks of the Tone 1-2 continuum and the /pa/-/pha/ continuum. Results revealed that children could perceive both lexical tones and aspiration of stops in a categorical manner by age four. The boundary position did not depend on age, with children having similar positions to adults regardless of speech continuum types. The boundary width, on the other hand, reached the adult-like level at age six for lexical tones, but not for stops. In addition, the within-category discrimination score did not differ significantly between children and adults for both continua. The between-category discrimination score improved with age and achieved the adult-like level at age five for lexical tones, but still not for stops even at age six. It suggests that the fine-grained perception of phonological categories is a protracted process, and the improvement and varying timeline of the development of segments and suprasegments are discussed in relation to statistical learning of the regularities of speech sounds in ambient language, ongoing maturation of perceptual systems, the memory mechanism underlying perceptual learning, and the intrinsic nature of speech elements.
Influencing Factors and Effects of Treatment on Quality of Life in Patients With Gastric Cancer—A Systematic Review
Authors: Rupp Sophia Kristina , Stengel Andreas
Published: July 2021

Background: Gastric cancer (GC) is one of the leading causes of death worldwide. It is associated with several disease-related impairments contributing to the psycho-social burden of those patients, such as deterioration of well-being and overall quality of life (QOL). The aim of this study is to present the wide range of factors potentially impacting patients' overall well-being and possible preventive interventions.

Methods: This systematic review was conducted in October 2020 with a search in the PubMed, MedLine, PsycInfo, and Google Scholar databases. We used the keywords “gastric cancer,” “gastric neoplasm,” and each of them combined with “quality of life,” “depression,” and “anxiety” to identify all relevant articles reporting about potential impact factors influencing the overall well-being of patients suffering from gastric cancer.

Results: Finally, 125,490 articles were found, of which 125,431 were excluded in several steps of screening. Inclusion criteria were studies carried out on human ≥18 years of age, studies in English or German language, clinical trials, registry-based studies, cohort studies, population-based studies, and certain titles and abstracts. After screening for eligibility 35 potential factors influencing overall well-being in patients with GC were identified and classified into 9 important categories: genetic condition, treatment method, blood markers, nutritional status, daily living, state of health, mental state, supportive care, and alternative treatment.

Conclusion: Since various factors are involved in the development of patients' overall well-being, timely treatment of psycho-social impairments by physicians and psychologists is of enormous importance. Preventing psycho-social burden by improving patients' QOL should be of high importance in the treatment regimen of patients with GC.
Validation of the Lifetime Incidence of Traumatic Events (LITE-S/P) Questionnaires in Children and Adolescents in Slovenia
Authors: Uršič Katarina , Bucik Valentin , Klemenčič Simona , Bratina Nataša , Battelino Tadej , Dovč Klemen , Drobnič Radobuljac Maja
Published: July 2021

Introduction: A traumatic event is an extremely threatening and frightening experience in an individual's life. Children who are exposed to traumatic events are twice as likely to develop a mental disorder. Screening can provide insight into the traumatic experience of children, identifying those eligible for further evaluation, and support. With this aim, we evaluated the psychometric properties of the Lifetime Incidence of Traumatic Events questionnaire (LITE) in Slovene by calculating retest reliabilty, construct validity (cross-informant agreement) and external validity, where we calculated the correlation of the number of differenet traumatic events with psychopathological symptoms.

Methods: 280 child-parent pairs (children aged 11.3 ± 2.2 years) from various Slovenian primary schools participated in the study. They were divided into two groups: 180 healthy primary school students and 100 children with Type 1 Diabetes (our study was a part of a larger study The Influence of Psychobiological Adversity on Children and Adolescents with Type 1 Diabetes Study). Two versions of the LITE questionnaire were used. Children completed the child report (LITE-S) and parents the parent report (LITE-P) version. After 4 weeks, 117 children, and 114 parents filled out the LITEs again. External validity was assessed using the Youth Self Report and Child Behaviour Checklist syndrome-oriented scales.

Results: Retest reliability for individual scales was r = 0.469–0.639 (ρ = 0.443–0.636; p < 0.001), but higher for individual items (κ = 0.263–0.821; p < 0.001). Correlations between reports from parents and children were r = 0.313–0.345 (ρ = 0.317–0.348; p < 0.001). The number of different events experienced by children correlated significantly with the measured depressive—anxiety, and posttraumatic stress disorder symptoms.

Conclusions: Based on our results, the LITE-S and LITE-P “All events” scale have acceptable psychometric properties for use in research and in clinical practise screening. We recommend looking at single items, taking into consideration the responses from both the child and the parent for more precise information. To improve the precision of the psychodiagnostic capacity of the questionnaire, further research on various populations should be performed.
A Novel Group Cognitive Behavioral Therapy Approach to Adult Non-rapid Eye Movement Parasomnias
Authors: O'Regan David , Nesbitt Alexander , Biabani Nazanin , Drakatos Panagis , Selsick Hugh , Leschziner Guy D. , Steier Joerg , Birdseye Adam , Duncan Iain , Higgins Seán , Kumari Veena , Stokes Paul R. , Young Allan H. , Rosenzweig Ivana
Published: July 2021

Background: Following the success of Cognitive Behavioral Therapy (CBT) for insomnia, there has been a growing recognition that similar treatment approaches might be equally beneficial for other major sleep disorders, including non-rapid eye movement (NREM) parasomnias. We have developed a novel, group-based, CBT-program for NREM parasomnias (CBT-NREMP), with the primary aim of reducing NREM parasomnia severity with relatively few treatment sessions.

Methods: We investigated the effectiveness of CBT-NREMP in 46 retrospectively-identified patients, who completed five outpatient therapy sessions. The outcomes pre- and post- CBT-NREMP treatment on clinical measures of insomnia (Insomnia Severity Index), NREM parasomnias (Paris Arousal Disorders Severity Scale) and anxiety and depression (Hospital Anxiety and Depression Scale), were retrospectively collected and analyzed. In order to investigate the temporal stability of CBT-NREMP, we also assessed a subgroup of 8 patients during the 3 to 6 months follow-up period.

Results: CBT-NREMP led to a reduction in clinical measures of NREM parasomnia, insomnia, and anxiety and depression severities [pre- vs. post-CBT-NREMP scores: P (Insomnia Severity Index) = 0.000054; P (Paris Arousal Disorders Severity Scale) = 0.00032; P (Hospital Anxiety and Depression Scale) = 0.037]. Improvements in clinical measures of NREM parasomnia and insomnia severities were similarly recorded for a subgroup of eight patients at follow-up, demonstrating that patients continued to improve post CBT-NREMP.

Conclusion: Our findings suggest that group CBT-NREMP intervention is a safe, effective and promising treatment for NREM parasomnia, especially when precipitating and perpetuating factors are behaviorally and psychologically driven. Future randomized controlled trials are now required to robustly confirm these findings.
Successful Combination Therapy of Trazodone and Fluvoxamine for Pica in Alzheimer's Disease: A Case Report
Authors: Kanamori Tadashi , Kaneko Yoshiyuki , Yamada Kouju , Suzuki Masahiro
Published: July 2021

Pica in Alzheimer's disease (AD) makes it difficult for caregivers to provide care. However, few effective medications have been reported for pica in AD. We report a case of AD with pica that was successfully improved by trazodone and fluvoxamine. An 80-year-old woman with AD was admitted to our hospital due to aggravated pica, including eating weeds in the facility's garden and eating a dishwashing sponge. Her pica was accompanied by oral tendency, prosopagnosia, and placidity. She took rivastigmine and memantine, but these were ineffective for her pica. She was given olanzapine and perospirone, but both were discontinued due to over-sedation and severe extrapyramidal symptoms, respectively. We then administered trazodone and fluvoxamine, both of which have demonstrated effectiveness for pica in frontotemporal dementia (FTD). Her pica behaviors then disappeared without daytime sleepiness. In this case, pica with oral tendency, which was accompanied by prosopagnosia and placidity, may be interpreted as a partial symptom of Klüver–Bucy syndrome (KBS). KBS is often seen in FTD, but also occurs in late-stage AD. Our case together with previous reports showing that trazodone and fluvoxamine were effective for pica in FTD suggest that the same common drug therapy may be successful in pica with oral tendency, regardless of the subtype of dementia.
Study Protocol for a Global Survey: Awareness and Preparedness of Hospital Staff Against Coronavirus Disease (COVID-19) Outbreak
Authors: Qarawi Ahmad Taysir Atieh , Ng Sze Jia , Gad Abdelrahman , Luu Mai Ngoc , AL-Ahdal Tareq Mohammed Ali , Sharma Akash , Huan Vuong Thanh , Vuong Nguyen Lam , Tawfik Gehad Mohamed , Hashan Mohammad Rashidul , Dumre Shyam Prakash , Ghozy Sherief , Shaikhkhalil Hosam Waleed , Mahmoud Mona Hanafy , Alhady Shamael Thabit Mohammed , Nam Nguyen Hai , Islam Sheikh Mohammed Shariful , Smith Chris , Lee Peter , Chico R. Matthew , Cox Sharon , Hirayama Kenji , Huy Nguyen Tien
Published: July 2021

Background: The outbreak of Coronavirus disease (COVID-19) caused by a novel coronavirus (named SARS-CoV-2) has gained attention globally and has been recognized as a Public Health Emergency of International Concern (PHEIC) by the World Health Organization (WHO) due to the rapidly increasing number of deaths and confirmed cases. Health care workers (HCWs) are vulnerable to this crisis as they are the first frontline to receive and manage COVID-19 patients. In this multicenter multinational survey, we aim to assess the level of awareness and preparedness of hospital staff regarding COVID-19 all over the world.

Methods: From February to March 2020, the web-based or paper-based survey to gather information about the hospital staff's awareness and preparedness in the participants' countries will be carried out using a structured questionnaire based on the United States Centers for Disease Control and Prevention (CDC) checklist and delivered to participants by the local collaborators for each hospital. As of March 2020, we recruited 374 hospitals from 58 countries that could adhere to this protocol as approved by their Institutional Review Boards (IRB) or Ethics Committees (EC).

Discussion: The awareness and preparedness of HCWs against COVID-19 are of utmost importance not only to protect themselves from infection, but also to control the virus transmission in healthcare facilities and to manage the disease, especially in the context of manpower lacking and hospital overload during the pandemic. The results of this survey can be used to inform hospitals about the awareness and preparedness of their health staff regarding COVID-19, so appropriate policies and practice guidelines can be implemented to improve their capabilities of facing this crisis and other future pandemic-prone diseases.
The Patterns and Predictors of Loneliness for the Chinese Medical Students Since Post-Lockdown to New Normal With COVID-19
Authors: Zhang Hui , Yang Jun , Li Yuxin , Ren Gaoyue , Mu Lina , Cai Yunjiang , Luo Qiusha , Zhou Yuqiu
Published: July 2021

Background: The coronavirus disease 2019 (COVID-19)-related quarantine has had unique psychological challenges for medical students, particularly loneliness. In this study, we demonstrated the patterns and predictors of loneliness in medical students since post-lockdown to new normal with COVID-19.

Methods: A convenience sampling method was used in this study. Face-to-face online questionnaires of UCLA Loneliness Scale and psychological characteristics scales were completed by 1,478 participants. Latent profile analysis and multinominal logistic regressions were performed.

Results: Three latent profile models were identified in this study: low loneliness (52.3%), interpersonal sensitivity loneliness (3.5%), and high loneliness (44.1%). Sophomore (Est = 1.937; p < 0.05) and junior students (Est = 2.939; p < 0.05), neuroticism (Est = 2.475; p < 0.05), high arousal symptoms (Est = 2.618; p < 0.01), and the quality of support from friends (Est = 2.264; p < 0.05) were the risk factors for high loneliness profile. In addition, sophomore (Est = 2.065; p < 0.05) and junior students (Est = 2.702; p < 0.01), openness (Est = 2.303; p < 0.05), and conscientiousness personality (Est = −2.348; p < 0.05) were the predictors of an interpersonal sensitive loneliness profile. Good peer relationship (Est = −2.266; p < 0.05) and other support (Est = −2.247; p < 0.05) were protective factors for low loneliness profile.

Limitations: Participants were selected from one medical university; the generalizability is limited.

Conclusions: Timely loneliness-focused interventions should be targeted on the different profiles and predictors of loneliness in medical students.
“This Graft-vs.-Host Disease Determines My Life. That's It.”—A Qualitative Analysis of the Experiences and Needs of Allogenic Hematopoietic Stem Cells Transplantation Survivors in Germany
Authors: Parisek Mira , Loss Julika , Holler Ernst , Barata Anna , Weber Daniela , Edinger Matthias , Wolff Daniel , Schoemans Helene , Herrmann Anne
Published: July 2021

Background:Allogeneic hematopoietic stem cell transplantation (alloHSCT) is the only curative treatment modality for many patients affected by hematologic malignancies. However, it can cause debilitating long-term effects. Understanding the impact of alloHSCT on all aspects of the patients' life is required for optimal survivorship management.

Aim:To explore in-depth HSCT-survivors' experiences and needs post-transplant. Partners were included to provide further information on survivors' needs and how care could be improved in this area.

Methods:We conducted semi-structured face-to-face and phone interviews with alloHSCT-survivors and their partners referred to a survivorship clinic in Germany. Theoretical sampling was used to recruit participants. Data were analyzed using framework analysis.

Results:Thirty-two survivors (consent rate: 100%, response rate: 100%) and eighteen partners (consent rate: 84%, response rate: 72%) participated. Survivors were aged between 25 and 68 years (Median: 48, IQR: 25.3) and partners were aged between 26 and 64 years (Median: 54, IQR: 16, SD: 12.8). The themes emerging from the data involved survivors' needs included (i) the diversity of long-term treatment side-effects; and (ii) time post discharge as a dynamic process with individual peaks of burden. Survivors and their partners also suggested strategies for mitigating these unmet needs, i.e., (iii) transparent communication and patient empowerment; and (iv) improvement in continuity of care system and help with claiming social benefits as cornerstones of optimal survivorship care.

Conclusion:To our knowledge, this is one of the first qualitative studies focused on the views of German alloHSCT-survivors on the long-term effects of alloHSCT and the first study integrating the view of their partners. Healthcare providers could better support survivors with managing their symptoms and adhering to their prescribed care by ensuring comprehensive, transparent communication that helps increase survivors' understanding and involvement in their care. Further efforts should be made to provide patient-centered, continuous survivorship care that involves additional support with navigating the healthcare and social service system. Intervention studies are required to test the effectiveness of the suggested strategies.
Optical-Tactile Sensor for Lump Detection Using Pneumatic Control
Authors: Bewley Jonathan , Jenkinson George P. , Tzemanaki Antonia
Published: July 2021

Soft tactile sensors are an attractive solution when robotic systems must interact with delicate objects in unstructured and obscured environments, such as most medical robotics applications. The soft nature of such a system increases both comfort and safety, while the addition of simultaneous soft active actuation provides additional features and can also improve the sensing range. This paper presents the development of a compact soft tactile sensor which is able to measure the profile of objects and, through an integrated pneumatic system, actuate and change the effective stiffness of its tactile contact surface. We report experimental results which demonstrate the sensor’s ability to detect lumps on the surface of objects or embedded within a silicone matrix. These results show the potential of this approach as a versatile method of tactile sensing with potential application in medical diagnosis.
Dynamically Tunable Friction via Subsurface Stiffness Modulation
Authors: Sharifi Siavash , Rux Caleb , Sparling Nathaniel , Wan Guangchao , Mohammadi Nasab Amir , Siddaiah Arpith , Menezes Pradeep , Zhang Teng , Shan Wanliang
Published: July 2021

Currently soft robots primarily rely on pneumatics and geometrical asymmetry to achieve locomotion, which limits their working range, versatility, and other untethered functionalities. In this paper, we introduce a novel approach to achieve locomotion for soft robots through dynamically tunable friction to address these challenges, which is achieved by subsurface stiffness modulation (SSM) of a stimuli-responsive component within composite structures. To demonstrate this, we design and fabricate an elastomeric pad made of polydimethylsiloxane (PDMS), which is embedded with a spiral channel filled with a low melting point alloy (LMPA). Once the LMPA strip is melted upon Joule heating, the compliance of the composite structure increases and the friction between the composite surface and the opposing surface increases. A series of experiments and finite element analysis (FEA) have been performed to characterize the frictional behavior of these composite pads and elucidate the underlying physics dominating the tunable friction. We also demonstrate that when these composite structures are properly integrated into soft crawling robots inspired by inchworms and earthworms, the differences in friction of the two ends of these robots through SSM can potentially be used to generate translational locomotion for untethered crawling robots.
Local Production and Storage in Positive Energy Districts: The Energy Sharing Perspective
Authors: Fichera Alberto , Pluchino Alessandro , Volpe Rosaria
Published: July 2021

In response to the Positive Energy District transition, this paper proposes an energy tool for the modeling of energy sharing configurations among buildings equipped with energy production systems and distributive storages. The model is targeted for urban planners and energy policymakers and gives insights into the role of buildings in fostering the achievement of net-zero energy balances in districts when virtual or physical peer-to-peer configurations are established in the area. A real urban district is considered as a case study and the energy performances are measured against properly defined Key Performance Indicators. Results confirm the strategic role played by energy sharing among buildings in achieving self-sufficient and carbon-neutral areas. In particular, the insertion of storages allows not only for higher self-sufficiency of the area (by facilitating the coupling of production and demand) but also for higher distribution rates among buildings. However, photovoltaic insertion and storages should be appropriately balanced since it has been observed that at increasing the number of production and storage systems, the distribution is reduced in favor of autonomy, thus limiting the usefulness of an interconnected local distribution grid.