bluuebunny/crossref_metadata_2025 huggingface dataset:
To develop a prognostic model for neoadjuvant immunochemotherapy efficacy in esophageal squamous cell carcinoma by analyzing the immune microenvironment
Authors: Yehan Zhou , Sheng Qin , Hong Yang , Jiayu Li , Jun Hou , Juan Ji , Min Shi , Jiaxin Yan , Shangzhi Hu , Yi Wang , Qifeng Wang , Xuefeng Leng , Wenwu He , Xueyan Cheng , Yang Liu , Zongyao Huang
Published: April 2024

Objective

The choice of neoadjuvant therapy for esophageal squamous cell carcinoma (ESCC) is controversial. This study aims to provide a basis for clinical treatment selection by establishing a predictive model for the efficacy of neoadjuvant immunochemotherapy (NICT).

Methods

A retrospective analysis of 30 patients was conducted, divided into Response and Non-response groups based on whether they achieved major pathological remission (MPR). Differences in genes and immune microenvironment between the two groups were analyzed through next-generation sequencing (NGS) and multiplex immunofluorescence (mIF). Variables most closely related to therapeutic efficacy were selected through LASSO regression and ROC curves to establish a predictive model. An additional 48 patients were prospectively collected as a validation set to verify the model’s effectiveness.

Results

NGS suggested seven differential genes (ATM, ATR, BIVM-ERCC5, MAP3K1, PRG, RBM10, and TSHR) between the two groups (P < 0.05). mIF indicated significant differences in the quantity and location of CD3+, PD-L1+, CD3+PD-L1+, CD4+PD-1+, CD4+LAG-3+, CD8+LAG-3+, LAG-3+ between the two groups before treatment (P < 0.05). Dynamic mIF analysis also indicated that CD3+, CD8+, and CD20+ all increased after treatment in both groups, with a more significant increase in CD8+ and CD20+ in the Response group (P < 0.05), and a more significant decrease in PD-L1+ (P < 0.05). The three variables most closely related to therapeutic efficacy were selected through LASSO regression and ROC curves: Tumor area PD-L1+ (AUC= 0.881), CD3+PD-L1+ (AUC= 0.833), and CD3+ (AUC= 0.826), and a predictive model was established. The model showed high performance in both the training set (AUC= 0.938) and the validation set (AUC= 0.832). Compared to the traditional CPS scoring criteria, the model showed significant improvements in accuracy (83.3% vs 70.8%), sensitivity (0.625 vs 0.312), and specificity (0.937 vs 0.906).

Conclusion

NICT treatment may exert anti-tumor effects by enriching immune cells and activating exhausted T cells. Tumor area CD3+, PD-L1+, and CD3+PD-L1+ are closely related to therapeutic efficacy. The model containing these three variables can accurately predict treatment outcomes, providing a reliable basis for the selection of neoadjuvant treatment plans.
Case report: Non-EBV associated cerebral vasculitis and cerebral hemorrhage in X-linked lymphoproliferative disease
Authors: Li Bangtao , Chen Weiming , Cai Xiaodi , Hai Yuanping , Pang Qiming , Xiang Wei , Zhang Zhengzheng
Published: April 2024

X-linked lymphoproliferative disease (XLP) is a rare genetic disorder characterized by immune dysregulation. The three most common clinical phenotypes are EBV-associated infectious mononucleosis (FIM), abnormal gammaglobulinemia, and lymphoma. We present a rare case of XLP1 with neurovasculitis, which is non-EBV-related and involves multiple systems, a condition rarely seen in children. The patient initially presented with an unsteady gait, which progressively evolved into language and consciousness disorders. Additionally, CT scans revealed multiple nodules in the lungs. Subsequent genetic testing and brain tissue biopsy confirmed the diagnosis: XLP1-related cerebral vasculitis and cerebral hemorrhage. Tragically, during the diagnostic process, the child experienced a sudden cerebral hemorrhage and herniation, ultimately resulting in fatality. This case offers a comprehensive insight into XLP1-related cerebral vasculitis and cerebral hemorrhage, underscoring the significance of early diagnosis and prompt treatment, while also imparting valuable clinical experience and lessons to the medical community.
Trunk control and acute-phase multifactorial predictors of community mobility after stroke: a longitudinal observational study
Authors: Alomari Randah Ahmed , BinMulayh Ejlal Abdullah , Alqarni Abdullah Mohammad , Alsobhi Mashael , Chevidikunnan Mohamed Faisal , Basuodan Reem , Khan Fayaz
Published: April 2024

Introduction

Stroke is a detrimental condition associated with long-term functional impairments that restrict community reintegration, which is an indicator of successful post-stroke functional recovery and rehabilitation. Additionally, trunk control is an understudied factor that may contribute to community mobility and participation after stroke. This study aimed to identify predictors of community mobility among stroke survivors in the acute phase, with a primary focus on trunk control, in addition to exploring the mediating and moderating role of predictive factors.

Methods

A longitudinal observational study included 61 participants with acute stroke. Trunk control test (TCT) during sitting, stroke severity, quality of life, fear of falls, depression, and age was assessed during the acute phase as potential predictors. The community mobility outcome measure was assessed 3 months after baseline using the Reintegration to Normal Living Index (RNLI). Statistical analyses included correlation, linear regression, mediation, and moderation analyses.

Results

Trunk control test was the strongest predictor of RNLI among all factors (β = 0.72; 95%CI = 0.004–0.007; p ≤ 0.0001). Stroke severity, quality of life, fear of falls, and age significantly predicted RNLI (p < 0.01). Higher age was a significant moderator of the relationship between TCT and RNLI (β = 0.002; p < 0.001; 95% CI = 0.0001–0.0003).

Discussion

The findings highlight sitting trunk control impairment during the acute stage as a crucial predictor of reduced community mobility after stroke, where age over 60 years can moderate this relationship. The study emphasizes that addressing trunk control during early stroke rehabilitation may enhance community reintegration prospects.
Zuranolone for treatment of major depressive disorder: a systematic review and meta-analysis
Authors: Ahmad Abdullah , Awan Abdul Rafeh , Nadeem Natasha , Javed Aamir Shahid , Farooqi Mobeen , Daniyal Muhammed , Mumtaz Hassan
Published: April 2024

Background

Current treatment modalities for Major Depressive Disorder have variable efficacies and a variety of side effects. To amend this, many trials for short term, well tolerated monotherapies are underway. One such option is Zuranolone (SAGE-217), which is a recent FDA approved antidepressant for Post Partum depression (PPD) and is undergoing clinical trials for PPD, major depressive disorder (MDD) and essential tremors (ET).

Objectives

Pool currently available data that compare Zuranolone to Placebo for the treatment of Major Depressive Disorder and evaluate its efficacy and safety profile.

Methods

We retrieved data from PUBMED and SCOPUS from inception to July 2023. We included articles comparing Zuranolone or SAGE 217 with placebo in patients suffering from Major Depressive Disorder. Review Manager 5.4 was used to analyze the outcomes including changes in the Hamilton Depression Rating Scale (HAM-D), Hamilton Anxiety Rating Scale (HAM-A) and Montgomery–Åsberg Depression Rating Scale (MADRS) scores from baseline as well as any treatment emergent adverse events (TEAEs) and severe adverse events.

Results

Our review analyzed 4 trials and the data of 1,357 patients. Patients treated with Zuranolone indicated a statistically significant effect in the change from baseline in HAM-D score (p = 0.0009; MD [95% CI]: −2.03 [−3.23, −0.84]) as well as in MADRS score (p = 0.02; MD [95% CI]: −2.30[−4.31, −0.30]) and HAM-A score (p = 0.03; MD [95% CI]: −1.41[−2.70, −0.11]) on 15th day when compared to the Placebo group. Zuranolone was also significantly associated with a higher response rate (p = 0.0008; OR [95% CI]: 1.63[1.14, 2.35]) and higher remission rate (p = 0.03; OR [95% CI]: 1.65[1.05, 2.59]) when compared with the placebo. As for safety, Zuranolone was significantly associated with 1 or more TEAE (p = 0.006; RR [95% CI]: 1.14[1.04, 1.24]) but an insignificant association with side effects that lead to drug discontinuation (p = 0.70; RR [95% CI]: 1.18[0.51, 2.76]) and serious adverse events (p = 0.48; RR [95% CI]: 1.46 [0.52, 4.10]) when compared with placebo.

Conclusion

Zuranolone is an effective and safe drug for short course major depressive disorder monotherapy. It shows results in 14 days (compared to 2–4 weeks that SSRI’s take) and has anti-anxiolytic effects as well. However, only 4 trials have been used for the analysis and the sample size was small. The trials reviewed also cannot determine the long-term effects of the drug. More trials are needed to determine long term effects.
Nutritional care for cancer patients: are we doing enough?
Authors: Da Prat Valentina , Pedrazzoli Paolo , Caccialanza Riccardo
Published: April 2024

Malnutrition is associated with higher rates of surgical complications, increased anticancer treatment toxicities, longer hospital stays, higher healthcare costs, poorer patient quality of life, and lower survival rates. Nutritional support has been shown to improve all of these outcomes. However, the nutritional care of cancer patients is still suboptimal and several issues remain unresolved. Although the effectiveness of nutritional support depends on the timeliness of intervention, assessment of nutritional status is often delayed and perceived as unimportant. When diagnoses of malnutrition are made, they are rarely recorded in medical records. Hospitals lack medical staff dedicated to clinical nutrition, making it difficult to integrate nutritional care into the multidisciplinary management of cancer patients. Outside the hospital, nutritional support is hampered by heterogeneous reimbursement policies and a lack of adequate community nutrition services. In addition, an increasing number of patients are turning to potentially harmful “anti-cancer” diets as trust in medicine declines. Adopting mandatory nutrition screening, monitoring quality of care metrics, providing nutrition education to care providers, and implementing telehealth systems are some of the most urgent interventions that need to be established in the future.
The hidden costs of working from home: examining loneliness, role overload, and the role of social support during and beyond the COVID-19 lockdown
Authors: Fostervold Knut Inge , Ulleberg Pål , Nilsen Odd Viggo , Halberg Anne Marie
Published: April 2024

Objective

This study evaluated the impact of the number of days per week working from home (WFH) on employee loneliness during and 2 years after the COVID-19 lockdown, with a focus on role overload as a mediating factor and social support from coworkers as a moderating variable.

Methods

Data were collected via self-reports from a sample of 6,918 participants during the lockdown in January 2021 and 6,576 participants 2 years post-lockdown in January 2023.

Results

Analysis using a moderated mediation model showed that increased WFH days were associated with heightened loneliness during the lockdown, a link that weakened post-lockdown. Role overload served as a mediator, intensifying loneliness during WFH but less so after the lockdown. While higher social support was generally linked to reduced role overload and loneliness, it paradoxically intensified these issues in individuals with extensive WFH days.

Conclusions

The findings suggest that the number of days WFH can exacerbate loneliness and role overload among employees, with the effect being more pronounced during the lockdown. Employees with substantial social support faced more challenges as WFH duration increased. These results underscore the complex dynamics between WFH, social support, and employee wellbeing.
Minimally invasive surgical technique for unstable supracondylar humerus fractures in children (Gartland type III or IV)
Authors: Lee Chang-Hyun , Jung Sung-Taek , Park Chun-Gon , Kim Joonyeong , Kang Gyo Rim , Kim Sungmin
Published: April 2024

Background

Achieving and maintaining anatomical reduction during the treatment of pediatric humerus fractures, classified as Gartland type III or IV, presents a clinical challenge. Herein, we present a minimally invasive surgical approach using a novel and simple K-wire push technique that aids in achieving and maintaining anatomical reduction.

Methods

We reviewed data of children receiving treatment for supracondylar fractures of the humerus at our hospital between January 2016 and December 2020. Patients were divided into two groups based on the method of treatment: Group 1 was treated with the K-wire push technique, and Group 2 was treated with the standard technique as described by Rockwood and Wilkins. The medical records and radiographic images were reviewed. In total, 91 patients with Gartland types III and IV fractures were included, with 37 and 54 patients in Groups 1 and 2, respectively.

Results

The postoperative reduction radiographic parameters and Flynn scores at final follow-up were not significantly different between the two groups.

Conclusion

The minimally invasive K-wire push technique for unstable supracondylar fractures in children is a safe and effective alternative for improving reduction. Using this technique, complications can be minimized, and the requirement for open reduction can be reduced.
Loose-patch clamp analysis applied to voltage-gated ionic currents following pharmacological ryanodine receptor modulation in murine hippocampal cornu ammonis-1 pyramidal neurons
Authors: Bertagna Federico , Ahmad Shiraz , Lewis Rebecca , Silva S. Ravi P. , McFadden Johnjoe , Huang Christopher L.-H. , Matthews Hugh R. , Jeevaratnam Kamalan
Published: April 2024

Introduction

The loose-patch clamp technique was first developed and used in native amphibian skeletal muscle (SkM), offering useful features complementing conventional sharp micro-electrode, gap, or conventional patch voltage clamping. It demonstrated the feedback effects of pharmacological modification of ryanodine receptor (RyR)-mediated Ca2+ release on the Na+ channel (Nav1.4) currents, initiating excitation–contraction coupling in native murine SkM. The effects of the further RyR and Ca2+-ATPase (SERCA) antagonists, dantrolene and cyclopiazonic acid (CPA), additionally implicated background tubular-sarcoplasmic Ca2+ domains in these actions.

Materials and methods

We extend the loose-patch clamp approach to ion current measurements in murine hippocampal brain slice cornu ammonis-1 (CA1) pyramidal neurons. We explored the effects on Na+ currents of pharmacologically manipulating RyR and SERCA-mediated intracellular store Ca2+ release and reuptake. We adopted protocols previously applied to native skeletal muscle. These demonstrated Ca2+-mediated feedback effects on the Na+ channel function.

Results

Experiments applying depolarizing 15 ms duration loose-patch clamp steps to test voltages ranging from −40 to 120 mV positive to the resting membrane potential demonstrated that 0.5 mM caffeine decreased inward current amplitudes, agreeing with the previous SkM findings. It also decreased transient but not prolonged outward current amplitudes. However, 2 mM caffeine affected neither inward nor transient outward but increased prolonged outward currents, in contrast to its increasing inward currents in SkM. Furthermore, similarly and in contrast to previous SkM findings, both dantrolene (10 μM) and CPA (1 μM) pre-administration left both inward and outward currents unchanged. Nevertheless, dantrolene pretreatment still abrogated the effects of subsequent 0.5- and 2-mM caffeine challenges on both inward and outward currents. Finally, CPA abrogated the effects of 0.5 mM caffeine on both inward and outward currents, but with 2 mM caffeine, inward and transient outward currents were unchanged, but sustained outward currents increased.

Conclusion

We, thus, extend loose-patch clamping to establish pharmacological properties of murine CA1 pyramidal neurons and their similarities and contrasts with SkM. Here, evoked though not background Ca2+-store release influenced Nav and Kv excitation, consistent with smaller contributions of background store Ca2+ release to resting [Ca2+]. This potential non-canonical mechanism could modulate neuronal membrane excitability or cellular firing rates.
Differences in Albizia odoratissima genetic diversity between Hainan Island and mainland populations in China
Authors: An Qi , Feng Yuanheng , Yang Zhangqi , Hu La , Wu Dongshan , Gong Guifang
Published: April 2024

Background

This study aimed at exploring unique population genetic characteristics of Albizia odoratissima (Linn. f) Benth on Hainan Island to provide a scientific basis for its rational utilization and protection.

Methods

It analyzed the genetic diversity and structure of 280 individuals from 10 subpopulations of A. odoratissima from Hainan Island and Baise City using 16 expression sequence markers - simple sequence repeat markers.

Results

The genetic diversity of Hainan population (I = 0.7290, He = 0.4483) was lower than that of the Baise population (I = 0.8722, He = 0.5121). Compared with the Baise population (Nm = 2.0709, FST = 0.1077), the Hainan Island population (Nm = 1.7519, FST = 0.1249) exhibited lower gene flow and higher degree of genetic differentiation. Molecular variance and genetic differentiation analyses showed that the main variation originated from individuals within the subpopulation. There were significant differences in the genetic structure between Hainan and Baise populations. It grouped according to geographical distance, consistent with the Mantel test results (R2 = 0.77, p = 0.001). In conclusion, the genetic diversity of the island A. odoratissima population was lower than that distributed on land, the two populations exhibited obvious genetic structure differences. Both the degrees of inbreeding and genetic differentiation were higher in the island population than in the land population.
The system functions of direct democracy - a ranking of 103 countries in the world
Authors: Hornig Eike-Christian , Frommelt Christian
Published: April 2024

We introduce a new framework of the functions of direct democracy within a democratic political system. These are: (1) channeling political input into the decision-making arenas by circumventing organized interests (input function), (2) channeling political issues out of the representative decision-making system (exit function), and (3) producing decisions about political questions (decision function). Based on the analysis of 277 instruments of direct democracy on the national level in 103 countries around the world, we find that the input and exit functions are rather unbalanced in their dynamics. The exit function shows a tendency to strengthen the concentration of power, whereas the input function rarely allows for access to both decision-making arenas. However, once issues reach the referendum, the impact is often strong, and only a small minority of states conduct referendums without any formal consequences. Beyond these results, the presented approach allows to start long-time observations of the state of direct democracy in democratic systems.
Synchrony or asynchrony: development of facial expression recognition from childhood to adolescence based on large-scale evidence
Authors: Wang Yihan , Luo Qian , Zhang Yuanmeng , Zhao Ke
Published: April 2024

The development of facial expression recognition ability in children is crucial for their emotional cognition and social interactions. In this study, 510 children aged between 6 and 15 participated in a two forced-choice task of facial expression recognition. The findings supported that recognition of the six basic facial expressions reached a relatively stable mature level around 8–9 years old. Additionally, model fitting results indicated that children showed the most significant improvement in recognizing expressions of disgust, closely followed by fear. Conversely, recognition of expressions of happiness and sadness showed slower improvement across different age groups. Regarding gender differences, girls exhibited a more pronounced advantage. Further model fitting revealed that boys showed more pronounced improvements in recognizing expressions of disgust, fear, and anger, while girls showed more pronounced improvements in recognizing expressions of surprise, sadness, and happiness. These clear findings suggested the synchronous developmental trajectory of facial expression recognition from childhood to adolescence, likely influenced by socialization processes and interactions related to brain maturation.
Epidemiology of physical activity and sedentary behavior levels among patients entering treatment for substance use disorder in the United States: a descriptive study
Authors: Churchill Sydney L. , Lansing Jeni E. , Brellenthin Angelique G. , Meyer Jacob D.
Published: April 2024

Introduction

Little is known about physical activity behaviors among people with SUD. This study aimed to (a) describe self-reported moderate-to-vigorous physical activity (MVPA) and sedentary (SED) behaviors of adults with SUD initiating treatment (b), determine the potential contributions of drug of choice (DOC) on these behaviors, and (c) determine the potential contributions of level of care and demographic variables on these behaviors.

Methods

Secondary data that was collected via surveys including demographic information, psychological health, drug of choice, MVPA (categorized as inactive, insufficiently active, meets guidelines, exceeds guidelines) and SED (<4 h/day, 4-<6 h/day, 6-8 h/day, >8 h/day) were analyzed from 1,293 patients in inpatient/outpatient treatment facilities across the United States.

Results

On average, over half (51%) of patients entering treatment reported not meeting guidelines, but sitting time was generally low (median= 360 min/day). MVPA levels differed based on level of care (p<0.001) with 48% of patients in detox facilities reporting inactivity compared to 37% in residential and 29% in outpatient programs. MVPA and SED levels differed by sex with women less likely to report sitting <4 h/day (27.9% vs. 38.2%, p<0.001) and more likely to report sitting for >8 h/day (31.5% vs. 21.8%, p<0.001) compared to men. SED differed by race (p=0.01), with 54% of Black patients reporting <4 h/day compared to 33% of White patients.

Discussion

Understanding activity behavior patterns among individuals entering SUD treatment provides opportunities for identifying the extent of lifestyle behavior needs and opportunities to develop personalized treatment strategies.
Increased brain gyrification and cortical thinning in winter-born patients with schizophrenia spectrum
Authors: Torigoe Misako , Takahashi Tsutomu , Takayanagi Yoichiro , Sasabayahi Daiki , Kobayashi Haruko , Sakamoto Kazumi , Yuasa Yusuke , Tsujii Noa , Noguchi Kyo , Suzuki Michio
Published: April 2024

Introduction

The findings of epidemiological studies suggest that a relationship exists between the risk of schizophrenia and winter births in the Northern Hemisphere, which may affect the process of fetal neurodevelopment. However, it remains unclear whether birth seasons are associated with the brain morphological characteristics of patients within the schizophrenia spectrum.

Methods

The present magnetic resonance imaging study using FreeSurfer software examined the effects of birth seasons (i.e., summer-born vs. winter-born) on the comprehensive brain surface characteristics of 101 patients with schizophrenia (48 summer- and 53 winter-born), 46 with schizotypal disorder (20 summer- and 26 winter-born), and 76 healthy control subjects (28 summer- and 48 winter-born).

Results

In comparisons with summer-born patients, winter-born patients, particularly those with schizophrenia, showed significantly increased gyrification mainly in the left lateral occipital and inferior temporal regions and right fronto-parietal region as well as cortical thinning in the right superior frontal region. Birth seasons did not significantly affect the local gyrification index or cortical thickness in healthy controls.

Discussion

The present whole-brain surface-based analysis demonstrated that brain morphological characteristics reported in the schizophrenia spectrum were more pronounced in winter-born patients than in summer-born patients, suggesting the contribution of early neurodevelopmental factors associated with birth seasons to the pathophysiology of the schizophrenia spectrum.
Clinical trial capacity building in a pandemic—outcome of a rapid site readiness project in Latin America
Authors: Clemens Sue Ann Costa , Gonzalez Isabela , Sereni Daniele , Clemens Ralf
Published: April 2024

Background

Latin America (Latam) has a tradition of large-scale vaccine trials. Because of fluctuating demand, many sites have downsized their infrastructure. Therefore, BMGF launched a clinical trial site-readiness initiative early in the coronavirus-2019 (COVID-19) pandemic including Latam countries between August and September 2020. This survey evaluated clinical development performance measures pre/post initiative (September 2022).

Results

20/21 prequalified sites participated in COVID-19 vaccine/drug development trials. 156 clinical trials (140 COVID-19 vaccine/drug trials) were initiated in the 2 years since prequalification, compared to 176 in the 5 years before. 33,428/37,810 participants were included in COVID-19 programs. The number of enrolled subjects/day across sites quadrupled from 15 (1–35) to 63 (5–300). The dropout rate was 6.8%. Study approval timelines were reduced from 60 (12–120) to 35 (5–90) days. Mean qualified staff was increased from 24 (6–80) to 88 (22–180).

Conclusion

Clinical trial sites across Latam were successfully prequalified to participate in COVID-19 developments. For the 100 days mission of vaccine availability in a new pandemic sufficient and well-trained clinical trial sites readily available are essential. This is only achievable if sites—especially in low/middle-income countries—are maintained active through a constant flow of vaccine studies.
Xputer: bridging data gaps with NMF, XGBoost, and a streamlined GUI experience
Authors: Younus Saleena , Rönnstrand Lars , Kazi Julhash U.
Published: April 2024

The rapid proliferation of data across diverse fields has accentuated the importance of accurate imputation for missing values. This task is crucial for ensuring data integrity and deriving meaningful insights. In response to this challenge, we present Xputer, a novel imputation tool that adeptly integrates Non-negative Matrix Factorization (NMF) with the predictive strengths of XGBoost. One of Xputer's standout features is its versatility: it supports zero imputation, enables hyperparameter optimization through Optuna, and allows users to define the number of iterations. For enhanced user experience and accessibility, we have equipped Xputer with an intuitive Graphical User Interface (GUI) ensuring ease of handling, even for those less familiar with computational tools. In performance benchmarks, Xputer often outperforms IterativeImputer in terms of imputation accuracy. Furthermore, Xputer autonomously handles a diverse spectrum of data types, including categorical, continuous, and Boolean, eliminating the need for prior preprocessing. Given its blend of performance, flexibility, and user-friendly design, Xputer emerges as a state-of-the-art solution in the realm of data imputation.
Influences of superfine-grinding and mix enzymatic hydrolysis combined with hydroxypropylation or acetylation on the structure and physicochemical properties of jujube kernel fiber
Authors: Zhang Jiangning , Ye Zheng
Published: April 2024

Introduction

Jujube kernel is a low-cost and abundant fiber resource, but its application in food industry is little because of its lower soluble fiber content and poor physicochemical properties.

Methods

In the current study, jujube kernel fiber (JKF) was modified by three composite methods: superfine-grinding and mix enzymatic hydrolysis alone, and combined with acetylation or hydroxypropylation.

Results and discussion

After these modifications, the microstructure of JKF became more porous, and its soluble fiber and extractable polyphenol contents, surface area, water adsorption and expansion capacities, and cation exchange capacity were all significantly improved (p < 0.05). Moreover, superfine-grinding and mix enzymatic hydrolysis combined with acetylation treated JKF showed the highest surface hydrophobicity (43.57) and adsorption ability to oil (4.47 g∙g−1). Superfine-grinding, mix enzymatic hydrolysis and hydroxypropylation treated JKF exhibited the largest surface area (142.53 m2∙kg−1), the highest soluble fiber content (17.43 g∙100 g−1), viscosity (14.54 cP), adsorption capacity to glucose (29.61 μmol∙g−1), cation exchange capacity (40.82), and water expansion ability (7.60 mL∙g−1). Therefore, superfine-grinding and mix enzymatic hydrolysis combined with hydroxypropylation or acetylation were both good choice to improve the physicochemical properties of JKF.
The use of a minimally invasive integrated endoscopic system to perform hemilaminectomies in chondrodystrophic dogs with thoracolumbar intervertebral disc extrusions
Authors: MacQuiddy Brittany , Bartner Lisa , Marolf Angela , Rao Sangeeta , Dupont Emily , Adams Taylor , Monnet Eric
Published: April 2024

Introduction

The objective was to evaluate the use of a minimally invasive surgical (MIS) approach to perform hemilaminectomies in chondrodystrophic dogs with thoracolumbar intervertebral disc extrusions (IVDE). Additionally, we aimed to evaluate the degree of soft tissue trauma using the endoscopic procedure compared to the standard open approach.

Methods

Eight client-owned dogs presented to the Colorado State University Veterinary Teaching Hospital with acute onset thoracolumbar IVDE were included in this study. This was a prospective, randomized case-series. Patients were assigned to undergo an endoscopic (group 1; n = 4) or a standard open approach (group 2; n = 4) for a hemilaminectomy. A post-operative MRI was performed in all cases.

Results

Conversion to an open approach was not necessary for any case in group 1. All cases had adequate spinal cord decompression on post-operative MRI. There was no significant difference in soft tissue changes noted on post-operative MRI between the two groups.

Discussion

The MIS approach to hemilaminectomies in chondrodystrophic dogs with thoracolumbar IVDE can successfully be performed to decompress the neural tissue and appears to lead to similar clinical outcomes in the early postoperative period compared to the standard open approach. Larger studies are needed to determine the potential advantages of the MIS technique compared to the standard open approach in veterinary medicine.
Metabolomics reveals early pregnancy biomarkers in sows: a non-invasive diagnostic approach
Authors: Ren Yujun , Zhang Qingze , He Fan , Qi Menfan , Fu Binbin , Zhang Huapeng , Huang Tao
Published: April 2024

In an effort to enhance reproductive management and reduce non-productive periods in swine breeding, this study presents a novel, non-invasive metabolomics approach for the identification of early pregnancy biomarkers in sows. Utilizing an untargeted metabolomics approach with mass spectrometry analysis, we examined saliva samples from pregnant (n = 6) and non-pregnant control sows (n = 6, artificially inseminated with non-viable sperm). Our analysis revealed 286 differentially expressed metabolites, with 152 being up-regulated and 134 down-regulated in the pregnant group. Among these, three metabolites, namely Hyodeoxycholic acid, 2′-deoxyguanosine, and Thymidine, emerged as potential early pregnancy biomarkers. These biomarkers were further evaluated using targeted LC–MS/MS quantification and qualification, accompanied by ROC curve analysis. The study confirmed Hyodeoxycholic acid and 2′-deoxyguanosine as promising biomarkers for early pregnancy detection, offering potential for future implementation in swine production environments. This research establishes a robust theoretical foundation for the development of innovative molecular diagnostic techniques and explores new avenues for molecular genetic breeding and non-invasive diagnostics, ultimately enhancing fertility and productivity in sow herds.
Spatial Analysis of Intercity Migration Patterns of China’s Rural Population: Based on the Network Perspective
Authors: Zhou Yihu , Chen Huiguang , Fang Tingting
Published: April 2024

Since entering the 21st century, many developing countries around the world have begun to enter a stage of rapid urbanization; large-scale “rural-urban” population migration has become a typical social phenomenon in these countries. Against this backdrop, this paper aims to elucidate the spatial migration characteristics of rural populations and to discuss future rural–urban development strategies. For this purpose, this paper takes China as a case and employs methods such as spatial autocorrelation analysis, hotspot analysis, and network analysis to construct an intercity migration network of rural migrants and analyze its spatial characteristics and internal structure. The results indicate that the migration pattern of the rural population exhibits notable spatial clustering features. Cities in the eastern and central regions are, respectively, hotspots for the inflow and outflow of rural populations, with internal migration dominating in western cities and relatively inactive rural population movements in northeastern cities. Municipalities directly under the central government, sub-provincial cities, and provincial capitals show a significant tendency to attract rural populations, while prefecture-level and county-level cities mainly radiate rural populations outward. Cities nationwide form seven major clusters in the migration network, and these clusters exhibit distinct structural characteristics. Rural population migration is influenced by various factors. In the future, considerations should focus on the county as the primary unit, attracting rural populations for local employment, and promoting rural revitalization and agriculture development. The findings of this paper are of reference significance not only to China but also to many developing countries with similar national conditions in the world.
Study on the Mechanism of Slow-Release Fertilizer and Nitrogen Fertilizer on the Senescence Characteristics of Quinoa Leaves
Authors: Lu Jingying , Zhang Qi , Sun Xiaojing , Deng Yan , Guo Hongxia , Wang Chuangyun , Zhao Li
Published: April 2024

The objective of this study was to investigate how nitrogen and slow-release fertilizers affect the traits of leaf senescence and quinoa production in order to explore the optimal slow-release fertilizer and nitrogen fertilizer ratios suitable for quinoa production, as well as to provide theoretical references for the planting of quinoa fertilization methods and fertilizer amount. In this experiment, the main local strain Quinoa 77 was selected as the experimental material, and six treatments were set up: CK: no nitrogen fertilizer; T1: 100% urea (N); T2: 100% slow-release fertilizer (C); T3: 5:5 (C5N5); T4: 3:7 (C3N7); and T5: 7:3 (C7N3). This was done in order to investigate how various treatments affect the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD); malondialdehyde (MDA) content; and yield of quinoa leaves. The findings revealed the following: (1) As the reproductive period progressed, the activities of CAT, POD, and SOD in quinoa leaves treated differently showed a tendency to increase and subsequently decrease, and they reached the peak value at the early stage of filling. The activity of CAT, POD, and SOD in the T3 treatment was the highest, and the average activities were 3148.74 U·g−1, 2197.84 U·g−1, and 118.51 U·g−1, respectively, which increased by 78.90%, 101.99%, and 108.14%, respectively, compared with CK. The content of MDA continued to increase with the progress of fertility. The average T3 treatment was 36.41 nmol·g−1, which was 46.87% lower than that of CK. (2) Out of all the treatments, T3 had the highest yield with an average of 3829.43 kg·hm−2, T5 the second with an average of 3313.52 kg·hm−2, and T4 the third with 2847.47 kg·hm−2, which increased yields by 96.18%, 69.75%, and 45.87%, respectively, compared with CK. (3) Yield was highly significantly and positively correlated with thousand kernel weight; number of grains per spike per plant; and the early filling stages of CAT, POD, and SOD sports, and it had a negative, extremely significant correlation with MDA content. Comprehensive analysis showed that slow-release fertilizer and nitrogen fertilizer can improve the antioxidant enzyme activity of quinoa leaves, inhibit MDA content, improve the physiological characteristics of quinoa, and delay the purpose of leaf senescence, with a better effect of yield and income, of which the T3 treatment had the high-quality impact of increasing yields and was a more scientific and reasonable fertilization method.
Soil Fertility and Bacterial Community Composition in Response to the Composting of Biochar-Amended Chicken Manure
Authors: Chen Longjun , Zhang Hui , Jia Xianbo , Fang Yu , Lin Chenqiang
Published: April 2024

Amidst the burgeoning expanse of the poultry sector, the escalation of chicken manure production has ensued, potentially exacerbating ecological contamination. However, the application of chicken manure is bound to transmute the habitat of edaphic microorganisms, precipitating an alteration in the soil’s microbial consortium. The composting of biochar-amended chicken manure and wood chips (biochar composting products, BCPs) was used to improve Chinese cabbage (Brassica campestris L.) production and regulate soil properties and bacterial community structure. On the 40th day of Chinese cabbage growth, soil and Chinese cabbage were collected for laboratory analysis. The effects of different proportions of BCPs (0, 1%, 3%, 5% and 7% biochar) on soil fertility, enzyme activity, the microbial community and the growth of Chinese cabbage were studied under facility conditions. The results showed that the growth performance and quality of Chinese cabbage were significantly increased with increasing BCP ratios. Soil fertility indicators including pH, AN, AP, AK and SOM were significantly increased, except for the pH value in the 1% BCP group. The activities of phosphatase, catalase and urease were increased for all groups of BCP treatment. The soil microbial community response was significantly different, and the application of 5% and 7% BCPs reduced the abundance, diversity and evenness of soil bacteria. Chinese cabbage growth performance was positively correlated with an increase in BCP supplemental levels in the range of 3–5%. Also, the abundance, diversity and uniformity of the soil microbial community were improved in the 3% BCP treatment group. Therefore, the dominant bacterial phyla were Bacteroidetes, Actinobacteriota, Gemmatimonadota, Myxococcota, Bdellovibrionota and Firmicutes, especially the Bradyrhizobium of Proteobacteria. BCP treatment reduced the degradation of soil organic matter. In addition, it also improved the relative abundance of sequences associated with improving soil fertility. Collectively, these findings offer insights for the re-evaluation of application management strategies for BCP organic fertilizers.
Cadaveric Study on Comparison of Neck Extension Angles for Endotracheal Intubation in Rabbits Using a Rigid and Flexible Endoscope
Authors: Luevitoonvechakij Nicharee , Buranapim Nithidol , Suriyasathaporn Witaya , Bansiddhi Pakkanut , Warrit Kanawee , Punyapornwithaya Veerasak , Suriyasathaporn Wanna
Published: April 2024

Endotracheal intubation in rabbits is always challenging due to the unique anatomical conformation. To improve endotracheal intubation success, this study determined the relationship between head placement angles guided by endoscope-assisted visualization techniques and the endotracheal intubation success rate. Thirty-two rabbit cadavers were used in the study. Six veterinary practitioners who had no experience with rabbit endotracheal intubation were randomly assigned to intubate rabbit cadavers using the guidance of either a rigid endoscope (RE) or flexible endoscope (FE), with the head placement angles with an ascending neck at 90, 100, 110, 120 and 130 degrees. The endotracheal intubation completed in 90 s was determined to be a success. The success rates using RE and FE were 97.2% and 95.9%, respectively. The means and standard error of means (SEM) of endotracheal intubation times guided by RE and FE were 53.7 ± 4.68 and 55.2 ± 4.24 s, respectively. Results from survival time analysis show that the five veterinarians successfully intubated the rabbit within 90 s, regardless of the different types of endoscopes. Angle was the only significant factor that affected the endotracheal intubation success. The head placement angle at 110 and 120 degrees had the highest success rate of endotracheal intubation compared to 90 degrees (p ≤ 0.05). In conclusion, for inexperienced veterinarians, the success of endotracheal intubation in rabbits, guided by endoscope-assisted visualized techniques regardless of rigid endoscope or flexible endoscope guidance, is improved when the head extension is 110 and 120 degrees.
Comparative Cranial Geometric Morphometrics among Wistar Albino, Sprague Dawley, and WAG/Rij Rat Strains
Authors: Ağaç Duygu Küçük , Onuk Burcu , Gündemir Ozan , Kabak Murat , Manuta Nicoleta , Çakar Buket , Janeczek Maciej , Crampton Denise Amber , Szara Tomasz
Published: April 2024

This research utilizes geometric morphometrics to investigate shape variation in the skull, mandible, and teeth among three rat strains: Wistar Albino (WA), Sprague Dawley (SD), and WAG/Rij (WR). Through the analysis of 48 rats using 2D geometric morphometric techniques, significant differences in their skull morphology were identified. This study indicates a shift from a rectangular to an oval cranial shape across strains, with notable size and morphological variances. Particularly, the WR strain’s skull shape significantly differs from the SD and WA strains, suggesting distinct ecological or genetic pathways. Compared to the skull, mandible shape differences are less pronounced, but still significant. The WR strain exhibits a distinct mandible shape, potentially reflecting ecological adaptations like dietary habits. The teeth shape of WR rats is the most distinct. SD rats consistently exhibited larger sizes in both skull and mandible measurements, while WR rats were notably smaller. Interestingly, sexual dimorphism was not statistically significant in skull and teeth sizes, aligning with findings from previous studies. However, the mandible showed clear size differences between sexes, underscoring its potential for adaptive or behavioral studies. In summary, this study provides a comprehensive analysis of morphological variations in rat strains, highlighting the intricate interplay of size, shape, and ecological factors. These findings lay a foundation for deeper explorations into the adaptive, ecological, or genetic narratives influencing rat morphology.
Polymorphisms of CYP7A1 and HADHB Genes and Their Effects on Milk Production Traits in Chinese Holstein Cows
Authors: Chen Ao , Yang Qianyu , Ye Wen , Xu Lingna , Wang Yuzhan , Sun Dongxiao , Han Bo
Published: April 2024

Our preliminary research proposed the cytochrome P450 family 7 subfamily A member 1 (CYP7A1) and hydroxyacyl-coenzyme A dehydrogenase trifunctional multienzyme complex beta subunit (HADHB) genes as candidates for association with milk-production traits in dairy cattle because of their differential expression across different lactation stages in the liver tissues of Chinese Holstein cows and their potential roles in lipid metabolism. Hence, we identified single-nucleotide polymorphisms (SNPs) of the CYP7A1 and HADHB genes and validated their genetic effects on milk-production traits in a Chinese Holstein population with the goal of providing valuable genetic markers for genomic selection (GS) in dairy cattle, This study identified five SNPs, 14:g.24676921A>G, 14:g.24676224G>A, 14:g.24675708G>T, 14:g.24665961C>T, and 14:g.24664026A>G, in the CYP7A1 gene and three SNPs, 11:g.73256269T>C, 11:g.73256227A>C, and 11:g.73242290C>T, in HADHB. The single-SNP association analysis revealed significant associations (p value ≤ 0.0461) between the eight SNPs of CYP7A1 and HADHB genes and 305-day milk, fat and protein yields. Additionally, using Haploview 4.2, we found that the five SNPs of CYP7A1 formed two haplotype blocks and that the two SNPs of HADHB formed one haplotype block; notably, all three haplotype blocks were also significantly associated with milk, fat and protein yields (p value ≤ 0.0315). Further prediction of transcription factor binding sites (TFBSs) based on Jaspar software (version 2023) showed that the 14:g.24676921A>G, 14:g.24675708G>T, 11:g.73256269T>C, and 11:g.73256227A>C SNPs could alter the 5′ terminal TFBS of the CYP7A1 and HADHB genes. The 14:g.24665961C>T SNP caused changes in the structural stability of the mRNA for the CYP7A1 gene. These alterations have the potential to influence gene expression and, consequently, the phenotype associated with milk-production traits. In summary, we have confirmed the genetic effects of CYP7A1 and HADHB genes on milk-production traits in dairy cattle and identified potential functional mutations that we suggest could be used for GS of dairy cattle and in-depth mechanistic studies of animals.
Arterial Blood Supply of the Stifle Joint in Horses
Authors: Schöpper Hanna , Egerbacher Monika
Published: April 2024

The vascularization pattern of the equine stifle joint is insufficiently described in the literature, even though there is a growing need for knowledge of the exact blood supply, as (i) arthroscopy and endoscopic surgery techniques are increasingly performed in horses and (ii) ex vivo models of menisci need nutrient supply that mimic the in vivo situation. The aim of this study was to describe the vessels involved in the stifle joint supply and the exact branching pattern of the popliteal artery. Colored latex was injected into the arteries of nine pelvic limbs of equine cadavers (n = 6) to evaluate the occurrences, variations and approximate diameters of vessels that supplied the stifle joints. Next to a branch of the saphenous and descending genicular arteries, eleven branches of the popliteal artery could be described in horses that feed the vascular network of the stifle joint. With a focus on the blood supply of the menisci, a vascularization map was created to show the main influx to these intra-articular structures in detail. These findings are potentially of great importance to both clinicians in preparation of best-suited incisions for arthroscopy and researchers designing new approaches for meniscal studies and choosing suitable animal models.
Characterization of a Trispecific PD-L1 Blocking Antibody That Exhibits EGFR-Conditional 4-1BB Agonist Activity
Authors: Rubio-Pérez Laura , Frago Susana , Compte Marta , Navarro Rocío , Harwood Seandean L. , Lázaro-Gorines Rodrigo , Gómez-Rosel Marina , Hangiu Oana , Silva-Pilipich Noelia , Vanrell Lucía , Smerdou Cristian , Álvarez-Vallina Luis
Published: April 2024

Immune checkpoint blockade has changed the treatment paradigm for advanced solid tumors, but the overall response rates are still limited. The combination of checkpoint blockade with anti-4-1BB antibodies to stimulate tumor-infiltrating T cells has shown anti-tumor activity in human trials. However, the further clinical development of these antibodies has been hampered by significant off-tumor toxicities. Here, we generated an anti-4-1BB/EGFR/PD-L1 trispecific antibody consisting of a triple-targeting tandem trimerbody (TT) fused to an engineered silent Fc region. This antibody (IgTT-4E1-S) was designed to combine the blockade of the PD-L1/PD-1 axis with conditional 4-1BB costimulation specifically confined to the tumor microenvironment (TME). The antibody demonstrated simultaneous binding to purified EGFR, PD-L1, and 4-1BB in solution, effective blockade of the PD-L1/PD1 interaction, and potent 4-1BB-mediated costimulation, but only in the presence of EGFR-expressing cells. These results demonstrate the feasibility of IgTT-4E1-S specifically blocking the PD-L1/PD-1 axis and inducing EGFR-conditional 4-1BB agonist activity.