bluuebunny/crossref_metadata_2025 huggingface dataset:
Electromobility and Flexibility Management on a Non-Interconnected Island
Authors: Mele Enea , Natsis Anastasios , Ktena Aphrodite , Manasis Christos , Assimakis Nicholas
Published: March 2021

The increasing penetration of electrical vehicles (EVs), on the way to decarbonizing the transportation sector, presents several challenges and opportunities for the end users, the distribution grid, and the electricity markets. Uncontrollable EV charging may increase peak demand and impact the grid stability and reliability, especially in the case of non-interconnected microgrids such as the distribution grids of small islands. On the other hand, if EVs are considered as flexible loads and distributed storage, they may offer Vehicle to Grid (V2G) services and contribute to demand-side management through smart charging and discharging. In this work, we present a study on the penetration of EVs and the flexibility they may offer for services to the grid, using a genetic algorithm for optimum valley filling and peak shaving for the case of a non-interconnected island where the electricity demand is several times higher during the summer due to the influx of tourists. Test cases have been developed for various charging/discharging strategies and mobility patterns. Their results are discussed with respect to the current generating capacity of the island as well as the future case where part of the electricity demand will have to be met by renewable energy sources, such as photovoltaic plants, in order to minimize the island’s carbon footprint. Higher EV penetration, in the range of 20–25%, is enabled through smart charging strategies and V2G services, especially for load profiles with a large difference between the peak and low demands. However, the EV penetration and available flexibility is subject to the mobility needs and limited by the population and the size of the road network of the island itself rather than the grid needs and constraints. Limitations and challenges concerning efficient V2G services on a non-interconnected microgrid are identified. The results will be used in the design of a smart charging controller linked to the microgrid’s energy management system.
Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces starkeyi and Chloroidium saccharophilum for Single Cell Oil Production
Authors: Zuccaro Gaetano , del Mondo Angelo , Pinto Gabriele , Pollio Antonino , De Natale Antonino
Published: March 2021

The mutualistic interactions between the oleaginous yeast Lipomyces starkeyi and the green microalga Chloroidium saccharophilum in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. The behavior of the two microorganisms alone and in mixed culture was studied in two synthetic media (YEG and BBM + G) before moving on to a real model represented by the hydrolysate of Arundo donax, used as low-cost feedstock, and previously subjected to steam explosion and enzymatic hydrolysis. The overall lipid content and lipid productivity obtained in the mixed culture of YEG, BBM + G and for the hydrolysate of Arundo donax were equal to 0.064, 0.064 and 0.081 glipid·gbiomass−1 and 30.14, 35.56 and 37.22 mglipid·L−1·day−1, respectively. The mixed cultures, in all cases, proved to be the most performing compared to the individual ones. In addition, this study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock, and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.
Special Issue: DNA Helicases: Mechanisms, Biological Pathways, and Disease Relevance
Authors: Brosh Robert M.
Published: March 2021

DNA helicases have emerged as a prominent class of nucleic acid-metabolizing enzymes that play important roles in genome maintenance and cellular homeostasis [...]
Nasopharyngeal Carriage of Methicillin-Resistant Staphylococcus aureus (MRSA) among Sickle Cell Disease (SCD) Children in the Pneumococcal Conjugate Vaccine Era
Authors: Dayie Nicholas T. K. D. , Sekoh Deborah N. K. , Kotey Fleischer C. N. , Egyir Beverly , Tetteh-Quarcoo Patience B. , Adutwum-Ofosu Kevin Kofi , Ahenkorah John , Osei Mary-Magdalene , Donkor Eric S.
Published: March 2021

The aim of this cross-sectional study was to investigate Staphylococcus aureus nasopharyngeal carriage epidemiology in relation to other nasopharyngeal bacterial colonizers among sickle cell disease (SCD) children about five years into pneumococcal conjugate vaccine 13 (PCV-13) introduction in Ghana. The study involved bacteriological culture of nasopharyngeal swabs obtained from 202 SCD children recruited from the Princess Marie Louise Children’s Hospital. S. aureus isolates were identified using standard methods and subjected to antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method. Cefoxitin-resistant S. aureus isolates were screened for carriage of the mecA, pvl, and tsst-1 genes using multiplex polymerase chain reaction. The carriage prevalence of S. aureus was 57.9% (n = 117), and that of methicillin-resistant S. aureus (MRSA) was 3.5% (n = 7). Carriage of the mecA, pvl, and tsst-1 genes were respectively demonstrated in 20.0% (n = 7), 85.7% (n = 30), and 11.4% (n = 4) of the cefoxitin-resistant S. aureus isolates. PCV-13 vaccination (OR = 0.356, p = 0.004) and colonization with coagulase-negative staphylococci (CoNS) (OR = 0.044, p < 0.0001) each protected against S. aureus carriage. However, none of these and other features of the participants emerged as a determinant of MRSA carriage. The following antimicrobial resistance rates were observed in MRSA compared to methicillin-sensitive S. aureus: clindamycin (28.6% vs. 4.3%), erythromycin (42.9% vs. 19.1%), tetracycline (100% vs. 42.6%), teicoplanin (14.3% vs. 2.6%), penicillin (100% vs. 99.1%), amoxiclav (28.6% vs. 3.5%), linezolid (14.3% vs. 0.0%), ciprofloxacin (42.9% vs. 13.9%), and gentamicin (42.9% vs. 13.0%). The proportion of S. aureus isolates that were multidrug resistant was 37.7% (n = 46). We conclude that S. aureus was the predominant colonizer of the nasopharynx of the SCD children, warranting the continuous monitoring of this risk group for invasive S. aureus infections.
Assessment of Optimism in Women with Polycystic Ovary Syndrome: A Case Control-Study
Authors: Morán-Sánchez Inés , Adoamnei Evdochia , Sánchez-Ferrer María L. , Prieto-Sánchez María T. , Arense-Gonzalo Julián J. , Carmona-Barnosi Ana , Hernandez-Peñalver Ana I. , Mendiola Jaime , Torres-Cantero Alberto M.
Published: March 2021

Polycystic ovary syndrome (PCOS) is a chronic endocrinopathy characterized by hyperandrogenism and anovulation that may pervade psychological dimensions such as dispositional optimism. Considering how PCOS influences mental health and the lack of studies on this matter, this research was aimed at assessing optimism and associated factors in PCOS. A case–control study of 156 patients with PCOS and 117 controls was conducted. All woman filled out the Life Orientation Test-Revised (LOT-R), a self-report questionnaire for measuring dispositional optimism. Medication, pain severity, gynecological, and sociodemographic information was also collected. Lower optimism was found in patients with PCOS compared to controls, even after covariate adjustment (LOT-R global scores: 14.1 vs. 15.9, p = 0.020). Our study provides evidence that a personality characteristic with important implications in illness prognosis may be affected in PCOS. We propose to assess dispositional optimism with the LOT-R scale in the gynecological appointment and tailor medical attention accordingly as a way to improve the comprehensive care of these patients within a multidisciplinary team.
Suitability of Measurement Tools for Assessing the Prevalence of Child Domestic Work: A Rapid Systematic Review
Authors: Pocock Nicola S. , Chan Clara W. , Zimmerman Cathy
Published: March 2021

Child domestic work (CDW) is a hidden form of child labour. Globally, there were an estimated 17.2 million CDWs aged 5–17 in 2012, but there has been little critical analysis of methods and survey instruments used to capture prevalence of CDW. This rapid systematic review identified and critically reviewed the measurement tools used to estimate CDWs in Low- and Middle-Income Countries, following PRISMA guidelines (PROSPERO registration: CRD42019148702). Fourteen studies were included. In nationally representative surveys, CDW prevalence ranged from 17% among 13–24-year-old females in Haiti to 2% of children aged 10–17 in Brazil. Two good quality studies and one good quality measurement tool were identified. CDW prevalence was assessed using occupation-based methods (n = 9/14), household roster (n = 7) and industry methods (n = 4). Six studies combined approaches. Four studies included task-based questions; one study used this method to formally calculate prevalence. The task-based study estimated 30,000 more CDWs compared to other methods. CDWs are probably being undercounted, based on current standard measurement approaches. We recommend use of more sensitive, task-based methods for inclusion in household surveys. The cognitive and pilot testing of newly developed task-based questions is essential to ensure comprehension. In analyses, researchers should consider CDWs who may be disguised as distant or non-relatives.
Energetic and Economic Evaluation of Zero-Waste Fish Co-Stream Processing
Authors: Venslauskas Kęstutis , Navickas Kęstutis , Nappa Marja , Kangas Petteri , Mozūraitytė Revilija , Šližytė Rasa , Župerka Vidmantas
Published: March 2021

This study evaluates the possibility of recovery of high-quality valuable fish oil and proteins from fish co-streams by traditional means or a combination of several technologies. A techno-economically feasible and sustainable zero-waste process is needed for full utilisation of this co-stream’s potential. This study aims to determine the energy efficiency and economic feasibility of four different zero-waste bio-refineries based on salmon filleting co-streams. The study covers four concepts: (I) biogas and fertiliser production from salmon co-streams, (II) fish silage production, (III) thermal processing of salmon co-streams for producing oil, protein concentrate, and meal, and (IV) novel two-stage thermal and enzymatic process for producing high-quality oil and protein hydrolysate, while the solid residue is converted to biogas and fertilisers. Monte Carlo simulation is used to evaluate uncertainties in economic evaluation. The results show that the two-stage processing of fish co-streams leads to recovery of both high-quality marine oil and proteins, showing the largest profitability and return on investment during the economic analysis. It is a more tempting option than the currently used thermal treatment or traditional silage processes. The possibility of producing food-grade fish protein hydrolysate is the biggest benefit here. Concepts studied are examples of zero-waste processing of bioproducts and illustrate the possibilities and benefits of fully utilising the different fractions of fish as fillets, oil, protein, fertilisers, and energy production.
Ethnic Identity as a Mediator of the Relationship between Discrimination and Psychological Well-Being in South—South Migrant Populations
Authors: Urzúa Alfonso , Caqueo-Urízar Alejandra , Henríquez Diego , Domic Marcos , Acevedo Daniel , Ralph Sebastian , Reyes Gonzalo , Tang Diego
Published: March 2021

There is abundant evidence about the negative impact of discrimination on well-being, but less research on factors that can reduce this negative effect, mainly focused on North American samples and with incipient development on South–South migration. The objective of this research was to analyze the effect of ethnic identity on the relationship between the experience of racial and ethnic discrimination and psychological well-being in Colombian immigrants living in Chile. A total of 962 immigrants over the age of 18 from three cities in Chile participated. Of these, 50.7% were women. The average age was 35 years (SD = 10.23). Participants were evaluated using Ryff’s Psychological Well-Being Scales, Phinney’s adapted version of the Multigroup Ethnic Identity Scale, and Krieger’s Discrimination Experience Scale. After the analysis of the measurement models, a mediation model was analyzed using structural equations. The results provide evidence that ethnic and racial discrimination have negative effects on psychological well-being, with the effect of racial discrimination being greater. Likewise, ethnic identity has positive effects on psychological well-being and partially and completely mediates the effects of ethnic and racial discrimination on psychological well-being. The full effect of discrimination on psychological well-being, mediated by ethnic identity, is exercised only by racial discrimination and not by ethnic discrimination.
Triangular Trajectory of Sustainable Development: Panel Analysis of the OECD Countries
Authors: Huh Taewook , Kim Yun Young
Published: March 2021

This study analyzes how the three pillars of sustainable development (economic growth, social justice, and environmental protection) have influenced each other for the past twenty-six years (from 1987 to 2013). The relationship between the triangular pillar of SD can be characterized by “ecological modernization”, “eco-socialism”, and the traditional debate between growth and distribution. This paper examined the correlation analysis of the nine representative variables in the three categories, adopting the cases of twenty-six OECD countries. In particular, the panel analysis (PCSE models) was conducted to identify the seven independent determinants affecting both response (dependent) variables and environmental factors (“CO2 emissions” and “renewable electricity output”). In short, during the entire period, the findings reveal that all economic and social variables did not have a positive impact on reducing CO2 emissions. However, the variables of “employment in industry” and “social expenditure” are effected by the increase of renewable electricity output. Consequently, highlighting the detailed findings different for each set period (1987–2013, 1987–2002, and 2003–2013), this study suggests the implications of the analysis result in the light of the theories of ecological modernization and eco-socialism.
The Role of Dopamine Receptor D2 in Bridging the Intention-Behavior Gap in Sport Participation
Authors: Park Seiyeong , Kwon Junhye , Ahn Chiyoung , Cho Hae-Sung , Moon Hyo Youl , Lee Chung Gun
Published: March 2021

Previous studies have identified that a behavior can occur through the strongest predictor intention, but there is a gap between intention and behavior. Dopamine receptor D2 (DRD2) is known to account for a variance in sporting behaviors in human and animal subjects. However, the relationship between DRD2 and sport participation has been poorly studied, and the limited available reports are inconsistent. The present study was performed to examine the impact of DRD2 on sport participation among Korean university students based on the integrated behavioral model (IBM). Data were collected from enrolled university students in Seoul (N = 45). Participants answered survey questions first, and then they gave investigators their hair to provide DNA information (i.e., the A1 allele of DRD2). DRD2 had a significant effect on sport participation, but only in male students. Male students who carried the A1 allele of DRD2 significantly participated in 105.10 min more sporting activities than male students who did not. Moreover, the effect of intention on sport participation was significantly decreased when considering DRD2. Despite the small sample size, the results of this study could be a preliminary case for a larger study and indicate the direction of future research. Our results suggest that DRD2 may have played an important role as the “actual skill” shown in the IBM.
Analysis on Effectiveness of Impact Based Heatwave Warning Considering Severity and Likelihood of Health Impacts in Seoul, Korea
Authors: Chae Yeora , Park Jongchul
Published: March 2021

Many countries are operating a heatwave warning system (HWWS) to mitigate the impact of heatwaves on human health. The level of heatwave warning is normally determined by using the threshold temperature of heat-related morbidity or mortality. However, morbidity and mortality threshold temperatures have not been used together to account for the severity of health impacts. In this study, we developed a heatwave warning system with two different warning levels: Level-1 and Level-2, by analyzing the severity and likelihood of heat-related morbidity and mortality using the generalized additive model. The study particularly focuses on the cases in Seoul, South Korea, between 2011 and 2018. The study found that the threshold temperature for heat-related morbidity and mortality are 30 °C and 33 °C, respectively. Approximately 73.1% of heat-related patients visited hospitals when temperature was between 30 °C and 33 °C. We validated the developed HWWS by using both the threshold temperatures of morbidity and mortality. The area under curves (AUCs) of the proposed model were 0.74 and 0.86 at Level-1 and Level-2, respectively. On the other hand, the AUCs of the model using only the mortality threshold were 0.60 and 0.86 at Level-1 and Level-2, respectively. The AUCs of the model using only the morbidity threshold were 0.73 and 0.78 at Level-1 and Level-2, respectively. The results suggest that the updated HWWS can help to reduce the impact of heatwaves, particularly on vulnerable groups, by providing the customized information. This also indicates that the HWWS could effectively mitigate the risk of morbidity and mortality.
A Meta-Analysis of the Efficacy of Hyaluronic Acid Eye Drops for the Treatment of Dry Eye Syndrome
Authors: Yang Yun-Jung , Lee Won-Young , Kim Young-jin , Hong Yeon-pyo
Published: March 2021

Hyaluronic acid (HA) is commonly used for treating dry eye syndrome (DES). This meta-analysis was performed to compare the efficacies of HA- and non-HA-based eye drops, including saline and conventional artificial tears (ATs), for the treatment of dry eye disease. Eight databases (PubMed, EMBASE, Cochrane Central Register of Controlled Trials, DBpia, KoreaMed, KMBASE, RISS, KISS) were searched for studies comparing the efficacies of HA- and non-HA-based ATs in patients with DES published up to September 2020. Two independent reviewers assessed the quality and extracted the relevant data. The mean differences of Schirmer’s (SH) test scores, tear breakup times (TBUT), corneal fluorescein staining scores (Oxford scale, 0–4), and ocular surface disease indexes were calculated. The standard mean difference and 95% confidence interval were calculated using a random effect model. Nineteen studies, including 2078 cases, were included. HA eye drops significantly improved tear production compared with non-HA-based eye drops (standard mean difference (SMD) 0.18; 95% confidence interval (CI) 0.03, 0.33). In a subgroup analysis, the SH test scores and TBUT values after using HA significantly increased compared to those measured after using saline (SMD 0.27; 95% CI 0.05, 0.49 and SMD 0.28; 95% CI 0.03, 0.52, respectively). Based on these results, HA eye drops may be superior to non-HA eye drops including normal saline and ATs. Further research is needed to assess the efficacies stratified by age, treatment duration, the severity of dry eye, and optimal dosages.
Microplastics: A Threat for Male Fertility
Authors: D'Angelo Stefania , Meccariello Rosaria
Published: March 2021

Much of the planet is swimming in discarded plastic, which is harming animal and possibly human health. Once at sea, sunlight, wind, and wave action break down plastic waste into small particles: the microplastics (MPs). Currently, particular attention has been drawn to their effects on aquatic environments but the health risks, especially in mammals, are poorly known. These non-biodegradable materials can act as a vector for environmental pollutants, can be ingested by humans in food and water, and can enter and accumulate in human tissues with a possible risk for heath. Recent studies revealed the deleterious effects of MPs exposure in male reproduction and sperm quality, making them a potential hazard to reproductive success. This manuscript summarizes the main changes in sperm quality along the lifespan and the upcoming studies on the effects of MPs in male fertility in mammals.
The Werther Effect, the Papageno Effect or No Effect? A Literature Review
Authors: Domaradzki Jan
Published: March 2021

This paper examines the association between media reporting on suicides and the subsequent suicides. Scientific papers from two online bibliographic sources Medline (PubMed) and PsycINFO were searched. The sample included 108 research papers examining the impact of different types of media stories on suicides. The review revealed that although the media can be a double-edged sword and serve both as a risk and a protective factor, the vast majority of research suggests that the relationship between the media reporting and the actual suicide rates is causal and real. Moreover, both the quantity and the quality of media reporting may trigger additional suicides in society. Simultaneously, research suggests that especially non-fictional presentations of celebrities’ suicides in newspapers and on television news have the biggest influence on the subsequent suicides. Additionally, a strong modelling effect of media reporting on suicide is based on nationality, age, and gender. However, research shows that because a negative reporting style can be modifiable and improved, the media can also have an educative or preventive effect and can reduce the risk of contagion. Consequently, it is important to monitor the implementation of media recommendations for the reporting of suicide, and continuous education of reporters is needed.
Loneliness Is Associated with Depressive Affect, But Not with Most Other Symptoms of Depression in Community-Dwelling Individuals: A Network Analysis
Authors: von Känel Roland , Weilenmann Sonja , Spiller Tobias R.
Published: March 2021

There is a strong relationship between loneliness and depression, but depression is a heterogeneous disorder. We examined the profile of depressive symptoms most strongly related to loneliness. Study participants were 2007 community-dwelling individuals (median age 31 years, 70.4% women) who completed an online survey on loneliness (single-item question: “never”, “sometimes”, “often”), depressive symptoms (Patient Health Questionnaire-9) and demographics. The relationship between loneliness and depressive symptoms was evaluated with linear regression and network analyses. The prevalence of loneliness (sometimes or often) and of moderate depression was 47.1% and 24.0%, respectively. Loneliness explained 26% of the variance in the total depressive symptom score (p < 0.001), independent of covariates. This result was almost exclusively explained by the relationship with a single depression symptom (“feeling down, depressed, or hopeless”), irrespective of whether loneliness was treated as a nominal or continuous variable. The findings of our study suggest that the role of loneliness in depression should not only be investigated at the syndrome level, but also at the symptom level. Studies are warranted to test whether targeted treatment of depressive affect is particularly effective against loneliness.
Fibrotic Phenotype of Peritumour Mesenteric Adipose Tissue in Human Colon Cancer: A Potential Hallmark of Metastatic Properties
Authors: Tabuso Maria , Adya Raghu , Stark Richard , Gopalakrishnan Kishore , Tsang Yee Wah , James Sean , White Andrew , Fisk Adrian , Dimitri Federica , Christian Mark , Arasaradnam Ramesh Pulendran
Published: March 2021

The impact of tumour associated stroma on cancer metastasis is an emerging field. However, cancer associated genes in peritumoral adipose tissue (pAT) in human colon cancer have not been explored. The aim of this study was to identify differentially expressed genes (DEGs) associated with cancer pathways in mesenteric pAT compared with adjacent adipose tissue. In total, nine patients with colon cancer pathological stage T2/T4 were employed in this study. DEGs were identified in 6 patients employing Nanostring PanCancer Pathway Panel and pathway enrichment analyses were performed. Differential expression of the 5 most up-regulated and 2 down regulated genes was validated with qRT-PCR. Results showed collagen type I alpha 1 chain (COL1A1) p = 0.007; secreted frizzled related protein (SFRP2) p = 0.057; fibroblast growth factor 7 (FGF7) not significant (ns); phospholipase A2, group IIA (PLA2G2A) ns; nerve growth factor receptor (NGFR) ns; lymphoid enhancer binding factor 1 (LEF1) p = 0.03; cadherin 1, Type 1, E-cadherin (epithelial) (CDH1) 0.09. Results have highlighted down-regulation of the Wingless/Integrated (Wnt) pathway in mesenteric pAT compared to distal adipose tissue. Highly upregulated genes in mesenteric pAT were involved in extracellular matrix (ECM)-receptor interactions and focal adhesion. Highly down regulated genes were involved in the cell cycle. Immunohistochemistry revealed differential distribution of COL1A1 showing maximum levels in tumour tissue and gradually decreasing in distant adipose tissue. COL1A1 and down regulation of Wnt pathway may have a role in local invasion and distant metastasis. COL1A1 may represent a stromal prognostic biomarker and therapeutic target in colon cancer.
Aggrephagy Deficiency in the Placenta: A New Pathogenesis of Preeclampsia
Authors: Nakashima Akitoshi , Shima Tomoko , Tsuda Sayaka , Aoki Aiko , Kawaguchi Mihoko , Furuta Atsushi , Yasuda Ippei , Yoneda Satoshi , Yamaki-Ushijima Akemi , Cheng Shi-Bin , Sharma Surendra , Saito Shigeru
Published: March 2021

Aggrephagy is defined as the selective degradation of aggregated proteins by autophagosomes. Protein aggregation in organs and cells has been highlighted as a cause of multiple diseases, including neurodegenerative diseases, cardiac failure, and renal failure. Aggregates could pose a hazard for cell survival. Cells exhibit three main mechanisms against the accumulation of aggregates: protein refolding by upregulation of chaperones, reduction of protein overload by translational inhibition, and protein degradation by the ubiquitin–proteasome and autophagy–lysosome systems. Deletion of autophagy-related genes reportedly contributes to intracellular protein aggregation in vivo. Some proteins recognized in aggregates in preeclamptic placentas include those involved in neurodegenerative diseases. As aggregates are derived both intracellularly and extracellularly, special endocytosis for extracellular aggregates also employs the autophagy machinery. In this review, we discuss how the deficiency of aggrephagy and/or macroautophagy leads to poor placentation, resulting in preeclampsia or fetal growth restriction.
Keratinocytes Migration Promotion, Proliferation Induction, and Free Radical Injury Prevention by 3-Hydroxytirosol
Authors: Abate Mario , Citro Marianna , Pisanti Simona , Caputo Mariella , Martinelli Rosanna
Published: March 2021

3-hydroxytyrosol (HT) is the main phenolic compound found in olive oil with known antioxidant, anti-inflammatory, and antimicrobial properties in several dermatological conditions, both when taken in the form of olive oil or pure in cosmeceutical formulations. To date, its direct effect on the wound healing process and the molecular mechanisms involved have not yet been elucidated. Thus, in the present study, we aimed to explore its effects in vitro in epidermal keratinocyte cultures focusing on the molecular mechanism implied. HT was able to induce keratinocyte proliferation in the low micromolar range, increasing the expression of cyclin dependent kinases fundamental for cell cycle progression such as CDK2 and CDK6. Furthermore, it increased cell migration through the activation of tissue remodeling factors such as matrix metalloproteinase-9 (MMP-9) protein. Then, we evaluated whether HT also showed antioxidant activity at this concentration range, protecting from H2O2-induced cytotoxicity. The HT prevented the activation of ATM serine/threonine kinase (ATM), Checkpoint kinase 1 (Chk1), Checkpoint kinase 2 (Chk2), and p53, reducing the number of apoptotic cells. Our study highlighted novel pharmacological properties of HT, providing the first evidence of its capability to induce keratinocyte migration and proliferation required for healing processes and re-epithelialization.
NGS-Based Application for Routine Non-Invasive Pre-Implantation Genetic Assessment in IVF
Authors: Gombos Katalin , Gálik Bence , Kalács Krisztina Ildikó , Gödöny Krisztina , Várnagy Ákos , Alpár Donát , Bódis József , Gyenesei Attila , Kovács Gábor L.
Published: March 2021

Although non-invasive pre-implantation genetic testing for aneuploidy (NIPGT-A) is potentially appropriate to assess chromosomal ploidy of the embryo, practical application of it in a routine IVF centre have not been started in the absence of a recommendation. Our objective in this study was to provide a comprehensive workflow for a clinically applicable strategy for NIPGT-A based on next-generation sequencing (NGS) technology with the corresponding bioinformatic pipeline. In a retrospective study, we performed NGS on spent blastocyst culture media of Day 3 embryos fertilised with intracytoplasmic sperm injection (ICSI) with quality score on morphology assessment using the blank culture media as background control. Chromosomal abnormalities were identified by an optimised bioinformatics pipeline applying copy number variation (CNV) detecting algorithm. In this study, we demonstrate a comprehensive workflow covering both wet- and dry-lab procedures supporting a clinically applicable strategy for NIPGT-A that can be carried out within 48 h, which is critical for the same-cycle blastocyst transfer. The described integrated approach of non-invasive evaluation of embryonic DNA content of the culture media can potentially supplement existing pre-implantation genetic screening methods.
Influence of Cu2+ on Osteoclast Formation and Activity In Vitro
Authors: Bernhardt Anne , Bacova Jana , Gbureck Uwe , Gelinsky Michael
Published: March 2021

Background: Copper-containing biomaterials are increasingly applied for bone regeneration due to their pro-angiogenetic, pro-osteogenetic and antimicrobial properties. Therefore, the effect of Cu2+ on osteoclasts, which play a major role in bone remodeling was studied in detail. Methods: Human primary osteoclasts, differentiated from human monocytes were differentiated or cultivated in the presence of Cu2+. Osteoclast formation and activity were analyzed by measurement of osteoclast-specific enzyme activities, gene expression analysis and resorption assays. Furthermore, the glutathione levels of the cells were checked to evaluate oxidative stress induced by Cu2+. Results: Up to 8 µM Cu2+ did not induce cytotoxic effects. Activity of tartrate-resistant acid phosphatase (TRAP) was significantly increased, while other osteoclast specific enzyme activities were not affected. However, gene expression of TRAP was not upregulated. Resorptive activity of osteoclasts towards dentin was not changed in the presence of 8 µM Cu2+ but decreased in the presence of extracellular bone matrix. When Cu2+ was added to mature osteoclasts TRAP activity was not increased and resorption decreased only moderately. The glutathione level of both differentiating and mature osteoclasts was significantly decreased in the presence of Cu2+. Conclusions: Differentiating and mature osteoclasts react differently to Cu2+. High TRAP activities are not necessarily related to high resorption.
Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111
Authors: Freedman Kimberley E. , Hill Jessica L. , Wei Yuren , Vazquez Allegra R. , Grubb Diana S. , Trotter Roxanne E. , Wrigley Scott D. , Johnson Sarah A. , Foster Michelle T. , Weir Tiffany L.
Published: March 2021

Probiotics make up a large and growing segment of the commercial market of dietary supplements and are touted as offering a variety of human health benefits. Some of the purported positive impacts of probiotics include, but are not limited to, stabilization of the gut microbiota, prevention of gastrointestinal disorders and modulation of the host immune system. Current research suggests that the immunomodulatory effects of probiotics are strain-specific and vary in mode of action. Here, we examined the immunomodulatory properties of Bacillus subtilis strain DE111 in a healthy human population. In a pilot randomized, double blind, placebo-controlled four-week intervention, we examined peripheral blood mononuclear cells (PBMCs) at basal levels pre- and post-intervention, as well as in response to stimulation with bacterial lipopolysaccharide (LPS). We observed an increase in anti-inflammatory immune cell populations in response to ex vivo LPS stimulation of PBMCs in the DE111 intervention group. Overall perceived gastrointestinal health, microbiota, and circulating and fecal markers of inflammation (Il-6, sIgA) and gut barrier function (plasma zonulin) were largely unaffected by DE111 intervention, although the study may have been underpowered to detect these differences. These pilot data provide information and justification to conduct an appropriately powered clinical study to further examine the immunomodulatory potential of B. subtilis DE111 in human populations.
Vitamin D Signaling in Gastro-Rheumatology: From Immuno-Modulation to Potential Clinical Applications
Authors: Pagnini Cristiano , Picchianti-Diamanti Andrea , Bruzzese Vincenzo , Lorenzetti Roberto , Luchetti Michele Maria , Martin Martin Louis Severino , Pica Roberta , Scolieri Palma , Scribano Maria Lia , Zampaletta Costantino , Chimenti Maria Sole , Lagana Bruno
Published: March 2021

In the last decades, the comprehension of the pathophysiology of bone metabolism and its interconnections with multiple homeostatic processes has been consistently expanded. The branch of osteoimmunology specifically investigating the link between bone and immune system has been developed. Among molecular mediators potentially relevant in this field, vitamin D has been recently pointed out, and abnormalities of the vitamin D axis have been described in both in vitro and in vivo models of inflammatory bowel diseases (IBD) and arthritis. Furthermore, vitamin D deficiency has been reported in patients affected by IBD and chronic inflammatory arthritis, thus suggesting the intriguing possibility of impacting the disease activity by the administration vitamin D supplements. In the present review, the complex interwoven link between vitamin D signaling, gut barrier integrity, microbiota composition, and the immune system was examined. Potential clinical application exploiting vitamin D pathway in the context of IBD and arthritis is presented and critically discussed. A more detailed comprehension of the vitamin D effects and interactions at molecular level would allow one to achieve a novel therapeutic approach in gastro-rheumatologic inflammatory diseases through the design of specific trials and the optimization of treatment protocols.
Oxidative Stress and ROS-Mediated Signaling in Leukemia: Novel Promising Perspectives to Eradicate Chemoresistant Cells in Myeloid Leukemia
Authors: Trombetti Silvia , Cesaro Elena , Catapano Rosa , Sessa Raffaele , Lo Bianco Alessandra , Izzo Paola , Grosso Michela
Published: March 2021

Myeloid leukemic cells are intrinsically under oxidative stress due to impaired reactive oxygen species (ROS) homeostasis, a common signature of several hematological malignancies. The present review focuses on the molecular mechanisms of aberrant ROS production in myeloid leukemia cells as well as on the redox-dependent signaling pathways involved in the leukemogenic process. Finally, the relevance of new chemotherapy options that specifically exert their pharmacological activity by altering the cellular redox imbalance will be discussed as an effective strategy to eradicate chemoresistant cells.
(Z)-5-(3′,4′-Bis(benzyloxy)benzylidene)furan-2(5H)-one
Authors: Artasensi Angelica , Baron Giovanna , Vistoli Giulio , Aldini Giancarlo , Fumagalli Laura
Published: March 2021

Over the years secondary metabolites have been considered as lead molecules both in their natural form and as templates for medicinal chemistry. Some secondary metabolites such as polyphenols and flavan-3-ols exert beneficial effects after a modification by the microbiota. Synthetic precursors of some of these modified compounds, in turn, carried a γ-alkylidenebutenolide moiety which characterizes a large class of bioactive natural products endowed with a wide range of biological activities. For these reasons stereoselective preparation of γ-alkylidenebutenolide continues to be an important issue for organic chemists. Our objective is to synthetize the novel compound (Z)-5-(3′,4′-bis(benzyloxy)benzylidene)furan-2(5H)-one in a stereocontrolled-one-pot reaction. The product was obtained in good yield. Furthermore, the theoretical investigation of the transition states suggests a new procedure to achieve Z-isomer of β-unsubstituted γ-alkylidenebutenolide.
Chloride Diffusion Property of Hybrid Basalt–Polypropylene Fibre-Reinforced Concrete in a Chloride–Sulphate Composite Environment under Drying–Wetting Cycles
Authors: Luo Yang , Niu Ditao , Su Li
Published: March 2021

The effect of fibre reinforcement on the chloride diffusion property of concrete is controversial, and the coupling effect of sulphate erosion and drying–wetting cycles in marine environments has been neglected in previous studies. In this study, the chloride diffusion property of hybrid basalt–polypropylene fibre-reinforced concrete subjected to a combined chloride–sulphate solution under drying–wetting cycles was investigated. The effects of basalt fibre (BF), polypropylene fibre (PF), and hybrid BP–PF on the chloride diffusion property were analysed. The results indicate that the presence of sulphate inhibits the diffusion of chloride at the early stage of erosion. However, at the late stage of erosion, sulphate does not only accelerate the diffusion of chloride by causing cracking of the concrete matrix but also leads to a decrease in the alkalinity of the pore solution, which further increases the risk of corrosion of the reinforcing steel. An appropriate amount of fibre can improve the chloride attack resistance of concrete at the early stage. With the increase in erosion time, the fibre effectively prevents the formation and development of sulphate erosion microcracks, thus reducing the adverse effects of sulphate on the resistance of concrete to chloride attack. The effects of sulphate and fibre on the chloride diffusion property were also elucidated in terms of changes in corrosion products, theoretical porosity, and the fibre-matrix interface transition zone.
Porosity Analysis of Additive Manufactured Parts Using CAQ Technology
Authors: Pokorný Peter , Václav Štefan , Petru Jana , Kritikos Michaela
Published: March 2021

Components produced by additive technology are implemented in various spheres of industry, such as automotive or aerospace. This manufacturing process can lead to making highly optimized parts. There is not enough information about the quality of the parts produced by additive technologies, especially those made from metal powder. The research in this article deals with the porosity of components produced by additive technologies. The components used for the research were manufactured by the selective laser melting (SLM) method. The shape of these components is the same as the shape used for the tensile test. The investigated parts were printed with orientation in two directions, Z and XZ with respect to the machine platform. The printing strategy was “stripe”. The material used for printing of the parts was SS 316L-0407. The printing parameters were laser power of 200 W, scanning speed of 650 mm/s, and the thickness of the layer was 50 µm. A non-destructive method was used for the components’ porosity evaluation. The scanning was performed by CT machine METROTOM 1500. The radiation parameters used for getting 3D scans were voltage 180 kV, current 900 µA, detector resolution 1024 × 1024 px, voxel size 119.43 µm, number of projections 1050, and integration time 2000 ms. This entire measurement process responds to the computer aided quality (CAQ) technology. VG studio MAX 3.0 software was used to evaluate the obtained data. The porosity of the parts with Z and XZ orientation was also evaluated for parts’ thicknesses of 1, 2, and 3 mm, respectively. It has been proven by this experimental investigation that the printing direction of the part in the additive manufacturing process under question affects its porosity.
Application of Silica Nanoparticles for Improving Growth, Yield, and Enzymatic Antioxidant for the Hybrid Rice EHR1 Growing under Water Regime Conditions
Authors: Elshayb Omnia M. , Nada Abdelwahed M , Ibrahim Heba M. , Amin Heba E. , Atta Ayman M.
Published: March 2021

The current study was designed to assess the effect of different concentrations of silica oxide nanoparticles (SiO2NPs) (0, 30, 60, and 90 ppm) as foliar applications under three irrigation regimes i.e., irrigation every 3 days (IR3, control), irrigation every 6 days (IR6), and irrigation every 9 days (IR9) on growth, yield and certain metabolites of rice (Oryza sativa L. cv. EHR1). To achieve such a goal, 2 field experiments were conducted during the 2018 and 2019 seasons at the Experimental Farm of Rice Research and Training Center (RRTC), Sakha Agricultural Station, Kafr El-sheik, Egypt. Firstly, the as-prepared nanoparticles of SiO2 were prepared from useless materials (RHs) which are considered as one of the bio burdens on the environment via treating with HCl and followed by drying and calcination. Consequently, the synthesis was examined by making use of advanced tools such as X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS) for illustrating the hydrodynamic particle size of SiO2NPs and scanning electron microscopy (SEM). The nanoparticles were formed with nearly spherical shape and small size. The results indicated that leaf area index, dry matter production, the number of panicles/m2, the number of filled grains/ panicles, 1000 grain weight, grain yield, and biological yield as well as chlorophyll content have witnessed a significant increase under irrigated application every 3 and 6 days. Whilst a prolonged irrigation regime up to 9 days recorded a remarkable decline in the aforementioned characteristics except for the number of unfilled grains/panicle which increased considerably in both seasons. On the other hand, proline concentration and the activity of the antioxidant enzymes were increased in both irrigated treatments every 6 and 9 days compared with control treatment (irrigation every 3 days). The foliar supplementations of (SiO2NPs) contributed to ameliorating all the aforementioned characteristics progressively up to the dosage of 90 ppm compared to control treatment (no Si/NPS application) in both seasons. Invariably, growth and yield parameters in water-stressed plants treated with SiO2NPs were higher than those in water-stressed plants without SiO2NPs addition. Based on that, it could be concluded that the foliar application of SiO2NPs can mitigate the adverse effect of water stress on rice plants.
Microlens Array Fabrication by Using a Microshaper
Authors: Lin Meng-Ju , Wen Cheng Hao
Published: March 2021

A simple, easy, inexpensive, and quick nonsilicon-based micromachining method was developed to manufacture a microlens array. The spherical surface of the microlens was machined using a microshaper mounted on a three-axis vertical computer numerical control (CNC) machine with cutter-path-planning. The results show the machined profiles of microlens agree well with designed profiles. The focus ability of the machined microlens array was verified. The designed and measured focal lengths have average 1.5% error. The results revealed that the focal lengths of micro lens agreed with the designed values. A moderate roughness of microlens surface is obtained by simply polishing. The roughness of the lens surface is 43 nm in feed direction (x-direction) and 56 nm in path interval direction (y-direction). It shows the simple, scalable, and reproducible method to manufacture microlenses by microshaper with cutter-path-planning is feasible.
Liquid Crystal Devices for Beam Steering Applications
Authors: Morris Rowan , Jones Cliff , Nagaraj Mamatha
Published: March 2021

Liquid crystals are valuable materials for applications in beam steering devices. In this paper, an overview of the use of liquid crystals in the field of adaptive optics specifically for beam steering and lensing devices is presented. The paper introduces the properties of liquid crystals that have made them useful in this field followed by a more detailed discussion of specific liquid crystal devices that act as switchable optical components of refractive and diffractive types. The relative advantages and disadvantages of the different devices and techniques are summarised.
Structural and Spectral Investigation of a Series of Flavanone Derivatives
Authors: Sykuła Anna , Kowalska-Baron Agnieszka , Gałęcki Krystian , Błazińska Paulina , Łodyga-Chruścińska Elżbieta
Published: March 2021

Four flavanone Schiff bases (E)-1-(2-phenylchroman-4-ylidene)thiosemicarbazide (FTSC) (1), N′,2-bis((E)-2-phenylchroman-4-ylidene)hydrazine-1-carbothiohydrazide (FTCH) (2), (E)-N’-(2-phenylchroman-4-ylidene)benzohydrazide (FHSB) (3) and (E)-N′-(2-phenylchroman-4-ylidene)isonicotinohydrazide (FIN) (4) were synthesized and evaluated for their electronic and physicochemical properties using experimental and theoretical methods. One of them, (2), consists of two flavanone moieties and one substituent, the rest of the compounds (1, 3, 4) comprises of a flavanone-substituent system in relation to 1:1. To uncover the structural and electronic properties of flavanone Schiff bases, computational simulations and absorption spectroscopy were applied. Additionally, binding efficiencies of the studied compounds to serum albumins were evaluated using fluorescence spectroscopy. Spectral profiles of flavanone Schiff bases showed differences related to the presence of substituent groups in system B of the Schiff base molecules. Based on the theoretically predicted chemical descriptors, FTSC is the most chemically reactive among the studied compounds. Binding regions within human and bovine serum albumins of the ligands studied are in the vicinity of the Trp residue and a static mechanism dominates in fluorescence quenching.