bluuebunny/crossref_metadata_2025 huggingface dataset:
Serological Survey of Canine Vector-Borne Infections in North-Center Spain
Authors: Pérez Pérez Patricia , Rodríguez-Escolar Iván , Carretón Elena , Sánchez Agudo José Ángel , Lorenzo-Morales Jacob , Montoya-Alonso José Alberto , Morchón Rodrigo
Published: December 2021

Various factors are currently causing an increase in vector-borne parasitic diseases at a global scale; among them, some stand out, such as climatic disturbances derived from global change, the increase in movements of reservoir animals, or changes in land made by human activity. In the European continent, there have been an increasing number of epidemiological studies focused on the detection of these diseases, especially in dogs. In Spain, there are few epidemiological studies focused on the evaluation of the biotic and abiotic factors that may influence the distribution, such as climatic zones, orography, or presence of water reservoirs. The aim of this study was to analyze the prevalence and distribution of several canine vector-borne diseases caused by Dirofilaria immitis, Leishmania infantum, Anaplasma platys, and Ehrlichia canis in the autonomous community of Castilla y León, the largest region of the Iberian Peninsula, providing a geospatial approach based on a geographic information system (GIS) analysis. Blood from a total of 1,475 domestic dogs from the nine provinces of Castilla y León were analyzed. Also, a GIS analysis of the sample locations was carried out, taking into account the most important predictor variables. The prevalence in dogs infected by D. immitis was 7.19%, and the seroprevalence by L. infantum was 4.61 and 1.56% for A. platys and E. canis. Most of the infected animals were located in areas with stagnant water, irrigated agriculture, or riverbanks, always close to forest and woodland vegetation. These results indicate that dogs living in Castilla y León should take prophylactic measures to avoid infections.
Clinical Features and Risk Factors for Gastrointestinal Complications in Dogs Treated Surgically for Thoracolumbar Intervertebral Disc Extrusion
Authors: Mehra Jaya M. , Tolbert M. Katherine , Moore George E. , Lewis Melissa J.
Published: December 2021

Gastrointestinal (GI) complications and their clinical implications are poorly characterized in dogs treated surgically for acute thoracolumbar intervertebral disc extrusion (TL-IVDE). The objective of this retrospective study was to characterize GI signs (including vomiting, diarrhea, melena, and hematochezia) in dogs undergoing hemilaminectomy for acute TL-IVDE. One-hundred and sixteen dogs were included. Frequency, type and severity of GI signs during hospitalization, duration of hospitalization and outcome were obtained from the medical record. Potential risk factors for the development of GI signs were explored using univariable and multivariable analyses. Gastrointestinal signs occurred in 55/116 dogs (47%); 22/55 dogs (40%) had one episode and 21/55 (38%) had ≥5 episodes. Diarrhea was the most common (40/55, 73%) while melena was rare (1/55, 2%). GI signs developed in 8/11 dogs (73%) treated perioperatively with both non-steroidal anti-inflammatories and corticosteroids with or without a washout period and in 25/52 dogs (48%) treated prophylactically with proton pump inhibitors. Median hospitalization was 7 days (4–15 days) vs. 5 days (4–11 days) in dogs with or without GI signs, respectively. Duration of hospitalization was associated with development of any GI signs, diarrhea and more severe GI signs (p= 0.001, 0.005, 0.021, respectively). Pre-operative paraplegia with absent pain perception was identified on univariable analysis (p= 0.005) and longer anesthetic duration on multivariable analysis to be associated with development of more severe GI signs (p= 0.047). In dogs undergoing surgery for acute TL-IVDE, GI signs were common and associated with duration of hospitalization and anesthesia. The influence of specific medications and neurologic severity on development of GI signs requires further investigation.
Back Electro Motive Force Estimation Method for Cascade Proportional Integral Control in Permanent Magnet Synchronous Motors
Authors: Gil Jeonghwan , You Sesun , Lee Youngwoo , Kim Wonhee
Published: December 2021

A cascade proportional integral control method with back-electro motive force compensation has been widely used for permanent magnet synchronous motors. In the permanent magnet synchronous motor control, it is important to accurately know the back-electro motive force constant for torque generation as well as back-electro motive force compensation. In this study, a real-time back-electro motive force constant estimation algorithm is developed to improve the velocity tracking control performance. The proposed method consists of a proportional integral controller and a back-electro motive force constant estimator. The proportional integral controller is designed to reduce the velocity tracking error. The back-electro motive force constant estimator is designed to estimate the back-electro motive force constant. It was verified that the estimated back-electro motive force constant converges to the actual back-electro motive force constant. The estimated back-electro motive force constant is applied to the cascade proportional integral controller. To verify the effectiveness of the proposed method, the performance of the proposed method is validated experimentally.
Output-Feedback Position Tracking Servo System with Feedback Gain Learning Mechanism via Order-Reduction Speed-Error-Stabilization Approach
Authors: You Sung Hyun , Kim Seok-Kyoon , Choi Hyun Duck
Published: December 2021

This paper presents a novel trajectory-tracking technique for servo systems treating only the position measurement as the output subject to practical concerns: system parameter and load uncertainties. There are two main contributions: (a) the use of observers without system parameter information for estimating the position reference derivative and speed and acceleration errors and (b) an order reduction exponential speed error stabilizer via active damping injection to enable the application of a feedback-gain-learning position-tracking action. A hardware configuration using a QUBE-servo2 and myRIO-1900 experimentally validates the closed-loop improvement under various scenarios.
Female Immigrant Entrepreneurship: The Experience of Chinese Migrants to Australia
Authors: Patrickson Margaret , Hallo Leonie
Published: December 2021

This article reports on findings from interviews with a small group of Chinese female immigrants to Australia who have started up their own business since their arrival. Unlike most publications concerning immigration that focus upon financial factors, we have instead concentrated on their personal journeys, why they started their businesses and the benefits they sought. We interviewed thirteen participants in Adelaide who had recently arrived from China with the aim of immigrating permanently to Australia. Immigration records indicate that by 2020 this figure had risen to over 160,000 per annum. However, it dropped again quickly in 2020 following the beginning of COVID-19. Nonetheless, according to recent Australian government records, over 866,200 current Australian residents have Chinese ancestry and 74% are first-generation migrants. The primary motivators for respondents were independence and control as well as income and skill development. Respondents were also satisfied by the personal development they gained.
Business Model Innovation: Strategic Expansion of German Small and Medium Wineries into Hospitality and Tourism
Authors: Dressler Marc , Paunovíc Ivan
Published: December 2021

This article provides insights into the modalities of business-model change and innovation. On the basis of an analysis of empirical data of small and medium enterprises, a transition from wine production centrism to its expanded use in hospitality and tourism is explored. Previous research on wine tourism and hospitality predominantly focuses on a destination perspective, neglecting the organizational winery perspective. The article deploys a mixed methods approach, combining netnography and a content analysis for data collection with grounded research and clustering for theory building. The sample size included 885 German wineries. Data stemmed from two distinct sources (websites and a secondary publication in form of a wine guide) and has been analyzed through a two-step clustering algorithm as well as a Principal Component Analysis (PCA). The two-step clustering algorithm resulted in nine different business models while the PCA analysis grouped the variables into the following two categories: basic winery business model (BM) and BM extension into hospitality and tourism, thereby validating the difference between the two constructs. The results point to the diverse nature of business model extensions of wineries in tourism and hospitality, depending on their organizational type and size. This study offers a classification of small and medium sized enterprise’s strategic business model expansion, and explores the expansion of the wine industry through wine hospitality and tourism services, starting with the winery organizational perspective, which has not been done before.
Statistical Analysis of Dynamic Subgrid Modeling Approaches in Large Eddy Simulation
Authors: Hossen Mohammad Khalid , Mulayath Variyath Asokan , Alam Jahrul M.
Published: December 2021

In large eddy simulation (LES) of turbulent flows, dynamic subgrid models would account for an average cascade of kinetic energy from the largest to the smallest scales of the flow. Yet, it is unclear which of the most critical dynamical processes can ensure the criterion mentioned above. Furthermore, evidence of vortex stretching being the primary mechanism of the cascade is not out of the question. In this article, we study essential statistical characteristics of vortex stretching. Our numerical results demonstrate that vortex stretching rate provides the energy dissipation rate necessary for modeling subgrid-scale turbulence. We have compared the interaction of subgrid stresses with the filtered quantities among four models using invariants of the velocity gradient tensor. The individual and the joint probability of vortex stretching and strain amplification show that vortex stretching rate is highly correlated with the energy cascade rate. Sheet-like flow structures are correlated with viscous dissipation, and vortex tubes are more stretched than compressed. The overall results indicate that the stretching mechanism extracts energy from the large-scale straining motion and passes it onto small-scale stretched vortices.
Aerodynamic Performance of a Coaxial Hex-Rotor MAV in Hover
Authors: Lei Yao , Wang Jiading , Yang Wenjie
Published: December 2021

Micro aerial vehicles (MAVs) usually suffer from several challenges, not least of which are unsatisfactory hover efficiency and limited fly time. This paper discusses the aerodynamic characteristics of a novel Hex-rotor MAV with a coaxial rotor capable of providing higher thrust in a compact structure. To extend the endurance during hover, flow field analysis and aerodynamic performance optimization are conducted by both experiments and numerical simulations with different rotor spacing ratios (i = 0.56, 0.59, 0.63, 0.67, 0.71, 0.77, 0.83, 0.91). The measured parameters are thrust, power, and hover efficiency during the experiments. Retip ranged from 0.7 × 105 to 1.3 × 105 is also studied by Spalart–Allmaras simulations. The test results show that the MAV has the optimum aerodynamic performance at i = 0.56 with Retip = 0.85 × 105. Compared to the MAV with i = 0.98 for Retip = 0.85 × 105, thrust is increased by 5.18% with a reduced power of 3.8%, and hover efficiency is also improved by 12.14%. The simulated results indicate a weakness in inter-rotor interference with the increased rotor spacing. Additionally, the enlarged pressure difference, reduced turbulence, and weakened vortices are responsible for the aerodynamic improvement. This provides an alternative method for increasing the MAV fly time and offers inspiration for future structural design.
Real-Time Detection of Rice Growth Phase Transition for Panicle Nitrogen Application Timing Assessment
Authors: Lai Joon-Keat , Lin Wen-Shin
Published: December 2021

Nitrogen (N) topdressing at the early reproductive phase (ER) is beneficial for rice yield. However, the ER overlaps with the late vegetative phase (LV) and is, thus, difficult to be recognized by human observation. Therefore, this study aimed to establish a high-temporal-resolution approach to determine the LV and ER via hyperspectral proximal sensing. Firstly, this research measured the leaf cover area (LCA), leaf dry weight (LDW), chlorophyll content (SPAD), leaf N content (LNC), and leaf N accumulation (LNA) to investigate the physical and physiological changes of the rice plant during growth phase transition. It could be summarized that the LCA would be maximally extended before ER, the leaf growth would be retarded after LV, and leaves turned from green to yellowish-green resulting from N translocation. These phenomena were expected to be detected by the hyperspectral sensor. In order to capture the variation of spectral information while eliminating redundant hyperspectral wavelengths, feature extraction (FE) and feature selection (FS) were conducted to reduce the data dimension. Meanwhile, the implications of the features were also inferenced. Three principal components, which correlated with the rice plant’s physical and physiological traits, were extracted for subsequent modeling. On the aspect of FS, 402, 432, 579, and 696 nm were selected as the predictors. The 402 nm wavelength significantly correlated with leaf cover area to some extent (p < 0.09), and 432 nm had no significant correlation with all of the measured plant traits (p > 0.10). The 579 nm and 696 nm wavelengths were negatively correlated with SPAD and LNC (p < 0.001). In addition, 696 nm was also negatively correlated with LNA (p < 0.05). Finally, the logistic regression, random forest (RF), and support vector machine (SVM) algorithms were adopted to solve the binary classification problem. The result showed that the feature extraction-based logistic regression (FE-logistic) and support vector machine (FE-SVM) were competent for growth phase discrimination (accuracy > 0.80). Nonetheless, taking the detrimental effects of applying N at LV into consideration, the feature extraction-based support vector machine (FE-SVM) was more appropriate for the timing assessment of panicle fertilizer application (sensitivity > 0.90; specificity > 0.80; precision > 0.80).
Repeated Manure Application for Eleven Years Stimulates Enzymatic Activities and Improves Soil Attributes in a Typic Hapludalf
Authors: Ferreira Paulo A. A. , Coronas Mariana V. , Dantas Max K. L. , Somavilla André , Brunetto Gustavo , Ceretta Carlos A. , Giacomini Sandro J. , Gubiani Paulo I. , Boitt Gustavo , Soares Claudio R. F. S. , Kaschuk Glaciela , Bordallo Samya U. , Lourenzi Cledimar R.
Published: December 2021

Animal manure may be a valuable resource for the development of agricultural sustainability. We proposed to verify the feasibility of applications of three types of animal manures to improve soil attributes and to sustain crop yields under intensive cropping and no-tillage systems. The field experiment was established in 2004 on Typic Hapludalf soil with pig slurry (PS), cattle slurry (CS), pig deep-litter (PL), mineral fertilizer (MF) and a non-fertilized treatment. From 2004 to 2015, were grown black oat, maize, forage turnip, black beans, and wheat. Soil samples were taken after winter 2014 and summer 2015, and submitted to chemical, physical, microbiological and biochemical analyses. Animal manures increased soil pH, but MF caused acidification of soil. The PL and CS applications reduced soil density, and increased total pore volume and hydraulic conductivity. Animal manures increased soil P fractions, total organic carbon, total nitrogen, stimulated soil respiration, and had higher activities of glucosidase and acid phosphatase. Wheat had its biggest dry matter and grain yields with MF, but maize grain yields with CS were higher than MF. All indicators pointed that application of animal manure converges to an interesting strategy to recycle nutrients at farmyard level and to contribute to global sustainability.
Changes in Soil-Borne Communities of Arbuscular Mycorrhizal Fungi during Natural Regrowth of Abandoned Cattle Pastures Are Indicative of Ecosystem Restoration
Authors: Rodríguez-León Carlos H. , Peña-Venegas Clara P. , Sterling Armando , Muñoz-Ramirez Herminton , Virguez-Díaz Yeny R.
Published: December 2021

Natural restoration of ecosystems includes the restoration of plant-microbial associations; however, few studies had documented those changes in tropical ecosystems. With the aim to contribute to understand soil microbial changes in a natural regrowth succession of degraded pastures that were left for natural restoration, we studied changes in arbuscular mycorrhizal (AM) fungal communities. Arbuscular mycorrhizal fungi (AMF) establish a mutualistic symbiosis with plants, improving plant nutrition. Amplification of the small subunit rRNA with specific primers and subsequent Illumina sequencing were used to search soil-borne AM fungal communities in four successional natural regrowth stages in two landscapes (hill and mountain) with soil differences, located in the Andean-Amazonian transition. Molecular results corroborated the results obtained previously by spores-dependent approaches. More abundance and virtual taxa of AMF exist in the soil of degraded pastures and early natural regrowth stages than in old-growth or mature forest soils. Although changes in AM fungal communities occurred similarly over the natural regrowth chronosequence, differences in soil texture between landscapes was an important soil feature differentiating AM fungal community composition and richness. Changes in soil-borne AM fungal communities reflect some signals of environmental restoration that had not been described before, such as the reduction of Glomus dominance and the increase of Paraglomus representativeness in the AM fungal community during the natural regrowth chronosequence.
Adoption and Profitability of the Purdue Improved Crop Storage Technology for Grain Storage in the South-Central Regions of Niger
Authors: Rabé Mahamane Moctar , Baoua Ibrahim B. , Baributsa Dieudonne
Published: December 2021

Cowpea is a food security crop and a main source of income for farmers in Niger. However, postharvest storage remains a major challenge due to insect pest attacks. Since 2008, the Purdue Improved Crop Storage (PICS) bags were disseminated in Niger to reduce storage losses. This study was conducted to assess the adoption of the PICS technology in the Dosso, Maradi, and Zinder regions of Niger. We interviewed 600 households selected from villages that did and did not benefit from PICS extension activities. A logit regression model was used to assess the decision of farmers to adopt the PICS technology. The overall adoption of the PICS bags among farmers was 48.4%. PICS adoption was 69.7% in Dosso, 41.3% in Zinder, and 31.2% in Maradi. Farmers who attended PICS training were 5 times more likely to adopt the technology than those who did not. Variables that affected the adoption of the PICS technology included the region, participation in PICS training, and information source. Beyond cowpea, PICS bags were used to store a variety of crops including Bambara nuts, hibiscus seeds, peanuts, millet, and sorghum. Storing 100 kg of cowpea in a PICS bag generated a cash flow of $70.38 per respondent and a net return of $21.50. Revenues generated from sales of cowpea stored in PICS bags were mostly used for health expenditures and to purchase agricultural inputs. Results of this study demonstrate that pest management technologies such as PICS bags can also contribute to improving the livelihood of family farms.
Experimental Applications and Factors Involved in Validating Thermal Windows Using Infrared Thermography to Assess the Health and Thermostability of Laboratory Animals
Authors: Verduzco-Mendoza Antonio , Bueno-Nava Antonio , Wang Dehua , Martínez-Burnes Julio , Olmos-Hernández Adriana , Casas Alejandro , Domínguez Adriana , Mota-Rojas Daniel
Published: December 2021

Evaluating laboratory animals’ health and thermostability are fundamental components of all experimental designs. Alterations in either one of these parameters have been shown to trigger physiological changes that can compromise the welfare of the species and the replicability and robustness of the results obtained. Due to the nature and complexity of evaluating and managing the species involved in research protocols, non-invasive tools such as infrared thermography (IRT) have been adopted to quantify these parameters without altering them or inducing stress responses in the animals. IRT technology makes it possible to quantify changes in surface temperatures that are derived from alterations in blood flow that can result from inflammatory, stressful, or pathological processes; changes can be measured in diverse regions, called thermal windows, according to their specific characteristics. The principal body regions that were employed for this purpose in laboratory animals were the orbital zone (regio orbitalis), auricular pavilion (regio auricularis), tail (cauda), and the interscapular area (regio scapularis). However, depending on the species and certain external factors, the sensitivity and specificity of these windows are still subject to controversy due to contradictory results published in the available literature. For these reasons, the objectives of the present review are to discuss the neurophysiological mechanisms involved in vasomotor responses and thermogenesis via BAT in laboratory animals and to evaluate the scientific usefulness of IRT and the thermal windows that are currently used in research involving laboratory animals.
Data-Based Variables Used as Indicators of Dairy Cow Welfare at Farm Level: A Review
Authors: Lutz Barbara , Zwygart Sibylle , Rufener Christina , Burla Joan-Bryce , Thomann Beat , Stucki Dimitri
Published: December 2021

During the last years, the interest in data-based variables (DBVs) as easy-to-obtain, cost-effective animal welfare indicators has continued to grow. This interest has led to publications focusing on the relationship between DBVs and animal welfare. This review compiles 13 papers identified through a systematic literature search to provide an overview of the current state of research on the relationship between DBVs and dairy cow welfare at farm level. The selected papers were examined regarding their definition of animal welfare and classified according to this definition into three categories: (a) papers evaluating DBVs as predictors of animal welfare violations, (b) papers investigating the relationship between DBVs and animal-based measurements, and (c) papers investigating the relationship of DBVs to scores of welfare assessments like the Welfare Quality protocol or to overall welfare scores at farm level. In addition, associations between DBVs and indicators of animal welfare were extracted, grouped by the type of DBV, and examined for replications that may confirm the associations. All the identified studies demonstrated associations between DBVs and animal welfare. Overall, the first indications of a possible suitability of DBVs for predicting herds with animal welfare violations as well as good or poor animal welfare status were given. The evaluation of relationships between DBVs and animal-based measurements (ABMs) found mortality-based DBVs to be frequently associated with ABMs. However, owing to varying definitions of animal welfare, the use of different variants of DBVs, and different methods used to assess DBVs, the studies could only be compared to a limited extent. Future research would benefit from a harmonisation of DBVs and the use of valid measurements that reflect the multidimensionality of welfare. Data sources rarely investigated so far may have the potential to provide additional DBVs that can contribute to the monitoring of cow welfare at farm level.
Age-Related Changes in Acute Phase Reaction, Cortisol, and Haematological Parameters in Ewes in the Periparturient Period
Authors: Greguła-Kania Monika , Kosior-Korzecka Urszula , Hahaj-Siembida Agata , Kania Konrad , Szysiak Natalia , Junkuszew Andrzej
Published: December 2021

A well-functioning immune system is the basis for protection against infectious and metabolic diseases, and a smooth return to homeostasis. The periparturient period is considered critical because major changes in the endocrine, behavioural, digestive, and immune systems dysregulate immune function, leading to immunosuppression. With age, the immune system could become dysregulated. The purpose of the present investigation was to compare changes in plasma concentrations of acute phase proteins, cortisol, and haematological parameters in the peripheral blood of two age-related groups of healthy ewes to get a better understanding of changes around lambing. Two groups of ewes were enrolled in the study: 3-year-old (young; n = 9) and 7-year-old ewes (old; n = 9). All females were synchronised and inseminated. In blood plasma, serum amyloid A (SAA) and cortisol concentrations were measured using ELISA tests, a spectrophotometric method to determine haptoglobin (Hp), and a thrombin clottable estimation to determine the fibrinogen (Fb) concentration. The blood parameters were examined using an automated haematological analyser. In clinically healthy ewes, no significant effect of age was observed in SAA, Hp, Fb and cortisol concentration in most of analysed terms. SAA, Hp, Fb, and cortisol fluctuations typical for the periparturient period were observed. There were no age-associated differences in red or white blood cell parameters.
Effects of Reduced Space Allowance and Heat Stress on Behavior and Eye Temperature in Unweaned Lambs: A Pilot Study
Authors: Menchetti Laura , Nanni Costa Leonardo , Zappaterra Martina , Padalino Barbara
Published: December 2021

Current European animal transportation law contains only a few and vague indications concerning how to move lambs of less than 26 kg. Moreover, little information is available in the literature about factors affecting these lambs’ welfare. We investigated the effect of space allowance and ambient temperature on the welfare of unweaned Lacaune lambs during a simulation of long-distance transportation (19 h). Three groups of lambs (N = 130) were housed in equally sized pens for 19 h, Control (C; n = 39; 0.27 m2 per head), Low Space Allowance (LSA; n = 52; 0.20 m2 per head), and Heat Stress (HS; n = 39; 0.27 m2 per head) groups. LSA lambs had lower space allowance than C but were tested at the same temperature, within their Thermoneutral zone (range = 12–18 °C). The HS lambs were, instead, subjected to higher temperatures (range = 19–30 °C). Scan sampling of behavior was conducted, eye temperature and body weight were also recorded. LSA and HS lambs showed more discomfort behaviors (p < 0.05) and higher eye temperatures (p < 0.001) compared to C lambs, while HS lambs additionally showed a decrease in body weight over the experimental period (p < 0.001). This study indicates that lower space allowances and higher temperatures impact negatively the welfare of lambs transported for slaughter suggesting that the regulation should be implemented taking these factors into account.
In Vitro Activities of Ceftazidime–Avibactam and Aztreonam–Avibactam at Different Inoculum Sizes of Extended-Spectrum β-Lactam-Resistant Enterobacterales Blood Isolates
Authors: Bae Moonsuk , Kim Taeeun , Park Joung Ha , Bae Seongman , Sung Heungsup , Kim Mi-Na , Jung Jiwon , Kim Min Jae , Kim Sung-Han , Lee Sang-Oh , Choi Sang-Ho , Kim Yang Soo , Chong Yong Pil
Published: December 2021

β-lactam–avibactam combinations have been proposed as carbapenem-sparing therapies, but little data exist on their in vitro activities in infections with high bacterial inocula. We investigated the in vitro efficacies and the inoculum effects of ceftazidime–avibactam and aztreonam–avibactam against extended-spectrum β-lactam-resistant Enterobacterales blood isolates. A total of 228 non-repetitive extended-spectrum β-lactam-resistant Escherichia coli and Klebsiella pneumoniae blood isolates were prospectively collected in a tertiary center. In vitro susceptibilities to ceftazidime, aztreonam, meropenem, ceftazidime–avibactam, and aztreonam–avibactam were evaluated by broth microdilution method using standard and high inocula. An inoculum effect was defined as an eightfold or greater increase in MIC when tested with the high inoculum. Of the 228 isolates, 99% were susceptible to ceftazidime–avibactam and 99% had low aztreonam–avibactam MICs (≤8 mg/L). Ceftazidime–avibactam and aztreonam–avibactam exhibited good in vitro activities; MIC50/MIC90 values were 0.5/2 mg/L, 0.125/0.5 mg/L, and ≤0.03/0.25 mg/L, respectively, and aztreonam–avibactam was more active than ceftazidime–avibactam. The frequencies of the inoculum effect with ceftazidime–avibactam and aztreonam–avibactam were lower than with meropenem (14% vs. 38%, p < 0.001 and 30% vs. 38%, p = 0.03, respectively). The β-lactam-avibactam combinations could be useful as carbapenem-sparing strategies, and aztreonam–avibactam has the better in vitro activity but is more subject to the inoculum effect than ceftazidime–avibactam.
Efficient Reduction in the Annual Investment Costs in AC Distribution Networks via Optimal Integration of Solar PV Sources Using the Newton Metaheuristic Algorithm
Authors: Montoya Oscar Danilo , Grisales-Noreña Luis Fernando , Alvarado-Barrios Lázaro , Arias-Londoño Andres , Álvarez-Arroyo Cesar
Published: December 2021

This research addresses the problem of the optimal placement and sizing of (PV) sources in medium voltage distribution grids through the application of the recently developed Newton metaheuristic optimization algorithm (NMA). The studied problem is formulated through a mixed-integer nonlinear programming model where the binary variables regard the installation of a PV source in a particular node, and the continuous variables are associated with power generations as well as the voltage magnitudes and angles, among others. To improve the performance of the NMA, we propose the implementation of a discrete–continuous codification where the discrete component deals with the location problem and the continuous component works with the sizing problem of the PV sources. The main advantage of the NMA is that it works based on the first and second derivatives of the fitness function considering an evolution formula that contains its current solution (xit) and the best current solution (xbest), where the former one allows location exploitation and the latter allows the global exploration of the solution space. To evaluate the fitness function and its derivatives, the successive approximation power flow method was implemented, which became the proposed solution strategy in a master–slave optimizer, where the master stage is governed by the NMA and the slave stage corresponds to the power flow method. Numerical results in the IEEE 34- and IEEE 85-bus systems show the effectiveness of the proposed optimization approach to minimize the total annual operative costs of the network when compared to the classical Chu and Beasley genetic algorithm and the MINLP solvers available in the general algebraic modeling system with reductions of 26.89% and 27.60% for each test feeder with respect to the benchmark cases.
Evaluating the Parameters of a Systematic Long-Term Measurement of the Concentration and Mobility of Air Ions in the Environment inside Císařská Cave
Authors: Roubal Zdeněk , Gescheidtová Eva , Bartušek Karel , Szabó Zoltán , Steinbauer Miloslav , Überhuberová Jarmila , Lajčíková Ariana
Published: December 2021

Determining the concentration and mobility of light air ions is an indispensable task to ensure the successful performance and progress of various operations within multiple fields and branches of human activity. This article discusses a novel methodology for measuring air ions in an environment with high relative humidity, such as that of a cave. Compared to common techniques, the proposed method exhibits a lower standard deviation and analyses the causes of spurious oscillations in the measured patterns obtained from FEM-based numerical simulations on the one hand and a model with concentrated parameters on the other. The designed ion meter utilises a gerdien tube to facilitate long-term measurement in cold and very humid spaces, an operation that can be very problematic if executed with other devices. Importantly, the applied procedure for calculating the mobility spectra of air ions from the acquired saturation characteristics is insensitive to fluctuations and noises in the measured patterns, and it also enables us to confirm the presence of very mobile air ions generated by fragmenting water droplets. During the sensing cycles, the concentration of light negative ions was influenced by the active gerdien tube. For the investigated cave, we had designed a measuring sequence to cover not only the time dependence of the concentration of light negative ions but also their mobility; this approach then allowed monitoring the corresponding impact of the patients’ presence in the cave, an effect neither described nor resolved thus far. Such comprehensive research, especially due to its specific character, has not been frequently conducted or widely discussed in the literature; the efforts characterised herein have therefore expanded the relevant knowledge and methodology, thus contributing towards further advancement in the field.
Oleg Zatsarinny: Expert Atomic Theorist, Kind Man
Authors: Gorczyca Thomas W.
Published: December 2021

I met Oleg Zatsarinny in 2001, and he then worked with me at Western Michigan University for two years. From 2003 to 2013, we were coauthors of 15 papers on theoretical atomic physics, and maintained a friendly relationship over twenty years, meeting and socializing often at conferences. Further elaboration follows below.
N-Acetylcysteine Reverses the Mitochondrial Dysfunction Induced by Very Long-Chain Fatty Acids in Murine Oligodendrocyte Model of Adrenoleukodystrophy
Authors: Zhou Jie , Terluk Marcia R. , Orchard Paul J. , Cloyd James C. , Kartha Reena V.
Published: December 2021

The accumulation of saturated very long-chain fatty acids (VLCFA, ≥C22:0) due to peroxisomal impairment leads to oxidative stress and neurodegeneration in X-linked adrenoleukodystrophy (ALD). Among the neural supporting cells, myelin-producing oligodendrocytes are the most sensitive to the detrimental effect of VLCFA. Here, we characterized the mitochondrial dysfunction and cell death induced by VLFCA, and examined whether N-acetylcysteine (NAC), an antioxidant, prevents the cytotoxicity. We exposed murine oligodendrocytes (158 N) to hexacosanoic acid (C26:0, 1–100 µM) for 24 h and measured reactive oxygen species (ROS) and cell death. Low concentrations of C26:0 (≤25 µM) induced a mild effect on cell survival with no alterations in ROS or total glutathione (GSH) concentrations. However, analysis of the mitochondrial status of cells treated with C26:0 (25 µM) revealed depletion in mitochondrial GSH (mtGSH) and a decrease in the inner membrane potential. These results indicate that VLCFA disturbs the mitochondrial membrane potential causing ROS accumulation, oxidative stress, and cell death. We further tested whether NAC (500 µM) can prevent the mitochondria-specific effects of VLCFA in C26:0-treated oligodendrocytes. Our results demonstrate that NAC improves mtGSH levels and mitochondrial function in oligodendrocytes, indicating that it has potential use in the treatment of ALD and related disorders.
Repetitive Trans Spinal Magnetic Stimulation Improves Functional Recovery and Tissue Repair in Contusive and Penetrating Spinal Cord Injury Models in Rats
Authors: Robac Amandine , Neveu Pauline , Hugede Alizée , Garrido Elisabeth , Nicol Lionel , Delarue Quentin , Guérout Nicolas
Published: December 2021

Spinal cord injury (SCI) is an incurable condition in which the brain is disconnected partially or completely from the periphery. Mainly, SCIs are traumatic and are due to traffic, domestic or sport accidents. To date, SCIs are incurable and, most of the time, leave the patients with a permanent loss of sensitive and motor functions. Therefore, for several decades, researchers have tried to develop treatments to cure SCI. Among them, recently, our lab has demonstrated that, in mice, repetitive trans-spinal magnetic stimulation (rTSMS) can, after SCI, modulate the lesion scar and can induce functional locomotor recovery non-invasively. These results are promising; however, before we can translate them to humans, it is important to reproduce them in a more clinically relevant model. Indeed, SCIs do not lead to the same cellular events in mice and humans. In particular, SCIs in humans induce the formation of cystic cavities. That is why we propose here to validate the effects of rTSMS in a rat animal model in which SCI leads to the formation of cystic cavities after penetrating and contusive SCI. To do so, several techniques, including immunohistochemical, behavioral and MRI, were performed. Our results demonstrate that rTSMS, in both SCI models, modulates the lesion scar by decreasing the formation of cystic cavities and by improving axonal survival. Moreover, rTSMS, in both models, enhances functional locomotor recovery. Altogether, our study describes that rTSMS exerts positive effects after SCI in rats. This study is a further step towards the use of this treatment in humans.
Identifying the Involvement of Pro-Inflammatory Signal in Hippocampal Gene Expression Changes after Experimental Ischemia: Transcriptome-Wide Analysis
Authors: Shishkina Galina T. , Gulyaeva Natalia V. , Lanshakov Dmitriy A. , Kalinina Tatyana S. , Onufriev Mikhail V. , Moiseeva Yulia V. , Sukhareva Ekaterina V. , Babenko Vladimir N. , Dygalo Nikolay N.
Published: December 2021

Acute cerebral ischemia induces distant inflammation in the hippocampus; however, molecular mechanisms of this phenomenon remain obscure. Here, hippocampal gene expression profiles were compared in two experimental paradigms in rats: middle cerebral artery occlusion (MCAO) and intracerebral administration of lipopolysaccharide (LPS). The main finding is that 10 genes (Clec5a, CD14, Fgr, Hck, Anxa1, Lgals3, Irf1, Lbp, Ptx3, Serping1) may represent key molecular links underlying acute activation of immune cells in the hippocampus in response to experimental ischemia. Functional annotation clustering revealed that these genes built the same clusters related to innate immunity/immunity/innate immune response in all MCAO differentially expressed genes and responded to the direct pro-inflammatory stimulus group. The gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses also indicate that LPS-responding genes were the most abundant among the genes related to “positive regulation of tumor necrosis factor biosynthetic process”, “cell adhesion”, “TNF signaling pathway”, and “phagosome” as compared with non-responding ones. In contrast, positive and negative “regulation of cell proliferation” and “HIF-1 signaling pathway” mostly enriched with genes that did not respond to LPS. These results contribute to understanding genomic mechanisms of the impact of immune/inflammatory activation on expression of hippocampal genes after focal brain ischemia.
Native llama Nanobody Library Panning Performed by Phage and Yeast Display Provides Binders Suitable for C-Reactive Protein Detection
Authors: Oloketuyi Sandra , Bernedo Robert , Christmann Andreas , Borkowska Justyna , Cazzaniga Giulia , Schuchmann Horst Wilhelm , Niedziółka-Jönsson Joanna , Szot-Karpińska Katarzyna , Kolmar Harald , de Marco Ario
Published: December 2021

C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher binder density on capture surfaces. Starting from the same pre-immune library, we compared the selection output resulting from two independent panning strategies, one exclusively exploiting the phage display and another in which a first round of phage display was followed by a second round of yeast display. There was a partial output convergence between the two methods, since two clones were identified using both panning protocols but the first provided several further different sequences, whereas the second favored the recovery of many copies of few clones. The isolated anti-CRP nanobodies had affinity in the low nanomolar range and were suitable for ELISA and immunoprecipitation. One of them was fused to SpyTag and exploited in combination with SpyCatcher as the immunocapture element to quantify CRP using electrochemical impedance spectroscopy. The sensitivity of the biosensor was calculated as low as 0.21 μg/mL.
Stem Cell Therapies for Cerebral Palsy and Autism Spectrum Disorder—A Systematic Review
Authors: Paprocka Justyna , Kaminiów Konrad , Kozak Sylwia , Sztuba Karolina , Emich-Widera Ewa
Published: December 2021

Autism spectrum disorder (ASD) and cerebral palsy (CP) are some of the most common neurodevelopmental diseases. They have multifactorial origin, which means that each case may manifest differently from the others. In patients with ASD, symptoms associated with deficits in social communication and characteristic, repetitive types of behaviors or interests are predominant, while in patients with CP, motor disability is diagnosed with accompanying cognitive impairment of various degrees. In order to minimize their adverse effects, it is necessary to promptly diagnose and incorporate appropriate management, which can significantly improve patient quality of life. One of the therapeutic possibilities is stem cell therapy, already known from other branches of medicine, with high hopes for safe and effective treatment of these diseases. Undoubtedly, in the future we will have to face the challenges that will arise due to the still existing gaps in knowledge and the heterogeneity of this group of patients. The purpose of this systematic review is to summarize briefly the latest achievements and advances in stem cell therapy for ASD and CP.
Numerical Investigation on Energy Efficiency of Heat Pump with Tunnel Lining Ground Heat Exchangers under Building Cooling
Authors: Liu Xiaohua , Li Chenglin , Zhang Guozhu , Zhang Linfeng , Wei Bin
Published: December 2021

For mountain tunnels, ground heat exchangers can be integrated into the tunnel lining to extract geothermal energy for building heating and cooling via a heat pump. In recent decades, many researchers only focused on the thermal performance of tunnel lining Ground Heat Exchangers (GHEs), ignoring the energy efficiency of the heat pump. A numerical model combining the tunnel lining GHEs and heat pump was established to investigate the energy efficiency of the heat pump. The inlet temperature of an absorber pipe was coupled with the cooling load of GHEs in the numerical model, and the numerical results were calibrated using the in situ test data. The energy efficiency ratio (EER) of the heat pump was calculated based on the correlation of the outlet temperature and EER. The heat pump energy efficiencies under different pipe layout types, pipe pitches and pipe lengths were evaluated. The coupling effect of ventilation and groundwater flow on the energy efficiency of heat pump was investigated. The results demonstrate that (i) the absorber pipes arranged along the axial direction of the tunnel have a greater EER than those arranged along the cross direction; (ii) the EER increases exponentially with increasing absorber pipe pitch and length (the influence of the pipe pitch and length on the growth rate of EER fades gradually as wind speed and groundwater flow rate increase); (iii) the influence of groundwater conditions on the energy efficiency of heat pumps is more obvious compared with ventilation conditions. Moreover, abundant groundwater may lead to a negative effect of ventilation on the heat pump energy efficiency. Hence, the coupling effect of ventilation and groundwater flow needs to be considered for the tunnel lining GHEs design.
Prognostic Role of PD-L1 Expression in Invasive Breast Cancer: A Systematic Review and Meta-Analysis
Authors: Cirqueira Magno Belém , Mendonça Carolina Rodrigues , Noll Matias , Soares Leonardo Ribeiro , de Paula Carneiro Cysneiros Maria Auxiliadora , Paulinelli Regis Resende , Moreira Marise Amaral Rebouças , Freitas-Junior Ruffo
Published: December 2021

Programmed death ligand 1 (PD-L1) has been investigated in various types of cancer; however, the role of PD-L1 expression in breast cancer remains controversial. We performed a systematic review and meta-analysis to assess the association of PD-L1 expression with clinicopathological variables, overall survival (OS), and disease-free survival (DFS) in invasive breast cancer. A total of 965 articles were included from CINAHL, Embase, PubMed, and Scopus databases. Of these, 22 studies encompassing 6468 cases of invasive breast cancer were included in the systematic review, and 15 articles were included in the meta-analysis. PD-L1 expression was associated with age ≥ 50 years, lymph node status-negative, progesterone receptor-negative, Ki67 ≥ 20%, and human epidermal growth factor receptor 2 (HER2)-negative. PD-L1 positivity was associated with worse OS (hazard ratio, HR, 2.39; 95% confidence interval, CI, 1.26–3.52; p =< 0.000); however, there was no significant improvement in DFS (HR 0.17; 95% CI −0.12–0.46; p =< 0.252). PD-L1 positivity was significantly associated with the clinicopathological characteristics of favorable and unfavorable prognoses. However, the final clinical outcome was associated with lower OS and had no significant association with DFS.
A Narrative Review of the Safety of Anti-COVID-19 Nutraceuticals for Patients with Cancer
Authors: Bader-Larsen Karlen Stade , Larson Elisabeth Anne , Dalamaga Maria , Magkos Faidon
Published: December 2021

Interest in dietary supplements and their efficacy in treating and preventing disease has increased greatly since the outbreak of the COVID-19 pandemic. Due to the risk of severe COVID-19 in patients with cancer, we conducted a narrative review aiming to better understand the data on the safety of the most efficacious “anti-COVID-19” nutraceuticals for patients with cancer. We conducted a PubMed database search aimed at identifying the most effective nutrients for use against COVID-19. For the identified nutraceuticals, we searched PubMed again regarding their safety for patients with cancer. Fifty-four total records (52 independent studies) were retrieved, pertaining to vitamin D, vitamin C, selenium, omega-3 fatty acids, and zinc. Vitamin D results from 23 articles indicated safe use, but two articles indicated potential harm. All 14 articles for vitamin C and five out of six articles for selenium indicated the safety of use (one study for selenium suggested harm with high-dose supplementation). Results for omega-3 fatty acids (seven articles) and zinc (one article), however, were rather mixed regarding safety. We conclude that vitamin D, vitamin C, and selenium supplements are likely safe or even beneficial at typically recommended doses; however, caution is urged with omega-3 fatty acid supplements, and zinc supplements should likely be avoided. More experimental research is needed, and nutraceutical use by patients with cancer should always be under the supervision of a healthcare team.