bluuebunny/crossref_metadata_2025 huggingface dataset:
A Collaborative Implementation Strategy to Increase Falls Prevention Training Using the Age-Friendly Health Systems Approach
Authors: Severance Jennifer Jurado , Rivera Solymar , Cho Jinmyoung , Hartos Jessica , Khan Amal , Knebl Janice
Published: May 2022

Falls in the home and in community environments are the leading cause of injuries and long-term disabilities for the aging population. The purpose of this study was to examine outcomes of a partnership among an academic institution, government agency, community organizations, and emergency management services to implement a falls prevention training program using an Age-Friendly Health Systems approach. In this prospective study, partners identified gaps in services and targeted and non-targeted delivery areas for implementation of an evidence-based falls prevention intervention addressing the 4Ms of Age-Friendly Health Systems—Mobility, Medications, Mentation, and What Matters. Descriptive statistics were calculated for program implementation and participant demographic variables, and paired t-test analysis compared scores for self-assessed general health and falls efficacy prior to and after program participation. Twenty-seven falls prevention classes were implemented, with over half (52%) in targeted areas. A total of 354 adults aged 50 and older participated, with N = 188 participants (53%) completing the program by attending at least five of eight sessions. Of completers, 35% resided in targeted areas. The results showed a statistically significant improvement in falls efficacy by program completers in targeted and non-targeted areas. However, there was no statistically significant difference in self-rated health. Overall, the findings of this study indicate that collaboration to deliver falls prevention training can be effective in reaching at-risk older adults. By mobilizing collaborative partnerships, limited resources can be allocated towards identifying at-risk older adults and improving community-based falls prevention education.
Improving the Technique of Pelvic Floor Muscle Contraction in Active Nulliparous Women Attending a Structured High–Low Impact Aerobics Program—A Randomized Control Trial
Authors: Piernicka Magdalena , Błudnicka Monika , Bojar Damian , Kortas Jakub , Szumilewicz Anna
Published: May 2022

Learning the correct technique of performing pelvic floor muscle (PFM) exercises is a very important factor influencing the effectiveness of this muscle group training. Correctly performed PFM contractions are involved in the urinary continence mechanism. In this study, we tested the hypothesis that a six-week high-low impact aerobics program, supported by one EMG biofeedback session and pelvic floor muscle training, improves the technique of PFM contraction. Participants were 42 active nulliparous women (age 22 ± 2 years, mean ± SD), randomly allocated into intervention (n = 18) and control (n = 24) groups. We analyzed the technique of PFM contractions, taking into account the order in which selected muscle groups were activated, so called ‘firing order’. In both groups, we assessed the PFM contraction technique using surface electromyography (sEMG) and intravaginal probes, before and after six weeks of intervention. The intervention group received one biofeedback session on how to properly contract PFM and afterwards participated in a high-low impact aerobics program supplemented by PFM training. The control group did not receive any intervention. In the pre-test, 67% of the intervention group activated PFM first in order in short, quick contractions. After six weeks of training, this task was correctly performed by 100% of this group (p = 0.04). The proper performance of PFM short contraction in the control group was 75% and 67%, before and after intervention, respectively. In the intervention group we also observed statistically significant improvement in the PFM contraction technique in 10-s contractions. The presented intervention was beneficial for the improvement of PFM contraction. High–low impact aerobics, supplemented by one EMG biofeedback session and pelvic floor muscle training can be recommended for active nulliparous women.
One-Size-Fits-All Policies Are Unacceptable: A Sustainable Management and Decision-Making Model for Schools in the Post-COVID-19 Era
Authors: Yang Cunwei , Wang Weiqing , Li Fengying , Yang Degang
Published: May 2022

This paper proposes a sustainable management and decision-making model for COVID-19 control in schools, which makes improvements to current policies and strategies. It is not a case study of any specific school or country. The term one-size-fits-all has two meanings: being blind to the pandemic, and conducting inflexible and harsh policies. The former strategy leads to more casualties and does potential harm to children. Conversely, under long-lasting strict policies, people feel exhausted. Therefore, some administrators pretend that they are working hard for COVID-19 control, and people pretend to follow pandemic control rules. The proposed model helps to alleviate these problems and improve management efficiency. A customized queue model is introduced to control social gatherings. An indoor–outdoor tracking system is established. Based on tracing data, we can assess people’s infection risk, and allocate medical resources more effectively in case of emergency. We consider both social and technical feasibility. Test results demonstrate the improvements and effectiveness of the model. In conclusion, the model has patched up certain one-size-fits-all strategies to balance pandemic control and normal life.
Analysis of Patients with Alcohol Dependence Treated in Silesian Intensive Care Units
Authors: Łowicka-Smolarek Małgorzata , Kokoszka-Bargieł Izabela , Knapik Małgorzata , Śmietanka Konstanty , Dyrda Piotr , Możdżeń Mateusz , Kurczab Magdalena , Borkowski Jarosław , Knapik Piotr
Published: May 2022

Analysis of patients with alcohol dependence (AD) treated in intensive care units has never been performed in Poland. Data from 25,416 adult patients identified in a Silesian Registry of Intensive Care Units were analysed. Patients with AD were identified, and their data were compared with the remaining population. Preadmission and admission variables that independently influenced ICU death in these patients were identified. Among 25,416 analysed patients, 2285 subjects (9.0%) were indicated to have AD among their comorbidities. Patients with AD were significantly younger (mean age: 53.3 ± 11.9 vs. 62.2 ± 15.5 years, p < 0.001) but had a higher mean APACHE II score at admission and were more frequently admitted to the ICU due to trauma, poisonings, acute pancreatitis, and severe metabolic abnormalities. ICU death and unfavourable outcomes were more frequent in these patients (47.8% vs. 43.0%, p < 0.001 and 54.1% vs. 47.0%, p < 0.001, respectively). Multiorgan failure as the primary cause of ICU admission was among the most prominent independent risk factors for ICU death in these patients (OR: 3.30, p < 0.001). Despite the younger age, ICU treatment of patients with AD was associated with higher mortality and a higher percentage of unfavourable outcomes.
Effects of Population Knowledge, Perceptions, Attitudes, and Practices on COVID-19 Infection Prevention and Control in NUST
Authors: Bosch Caitlin , Hauwanga Edwig , Omoruyi Beauty E. , Okeleye Benjamin I. , Okudoh Vincent I. , Aboua Yapo G.
Published: May 2022

The global COVID-19 pandemic has had a major impact on the education sector of most countries. One of the basic CDC prevention guidelines is the implementation of non-pharmaceutical interventions (NPIs) to protect the health of students and staff members to curve the spread of COVID-19. The current study aimed to examine the knowledge, perceptions, attitudes, and practices of students at the Namibia University of Technology toward the COVID-19 pandemic. A cross-sectional descriptive survey was conducted using a closed-ended questionnaire. Data were collected from full-time students who were on campus during the COVID-19 pandemic between 29 January to 14 February 2021. The average knowledge about the modes of transmission, protective measures, and clinical symptoms ranged from 78% to 96%. About 31% of student respondents believed the virus was created in a laboratory, and 47% believed the vaccine has negative side effects and therefore, refused to take it. The three main sources of information about COVID-19 were social media (75%), television (63%), and friends and family (50%). The students had an overall positive attitude towards the implementation of NPIs. However, the importance of vaccine safety must be emphasized. Lockdowns should be lifted gradually to reduce the amount of time students are spending on online content. Reopening of classrooms for face-to-face study will bring unquestionable benefits to students and the wider economy.
Mental Health and Addiction Services Exclusive to LGBTQ2S+ during COVID-19: An Environmental Scan
Authors: Chaiton Michael , Billington Rebecca , Copeland Ilana , Grey Luc , Abramovich Alex
Published: May 2022

Background: Youth who are lesbian, gay, bisexual, trans, queer, 2-spirit, and of other identities (LGBTQ2S+) experience mental health disparities and higher rates of substance use when compared to their cisgender and heterosexual peers and yet also experience more barriers to access to services. The purpose of this paper is to determine the types of mental health and substance use programs and services exclusive to LGBTQ2S+ youth in Ontario during the pandemic. Methods: An environmental scan was conducted to identify existing programs and services in Ontario, Canada that offered exclusive mental health and addiction services to LGBTQ2S+ individuals aged 16–29, either by offering services to all or subgroups within the population. Organizations, services and programs were classified by the geographical distribution of services, populations served, types of programming or services, methods of service delivery, and program criteria. Results: In total, 113 organizations and 240 programs and services were identified as providing mental health and substance use services exclusively to LGBTQ2S+ youth. Identified adaptations for the COVID-19 pandemic included cancelling in-person services, increasing online and telephone services, and expansion to province wide from local availability. Conclusions: The findings highlight the importance of offering services that provide culturally inclusive care for LGBTQ2S+ youth, and these results can also be used by policy makers to inform policies. In particular, there was a lack of culturally relevant clinical services for youth requiring a greater intensity of treatment.
Behavioral Outcomes of Children with Same-Sex Parents in The Netherlands
Authors: Mazrekaj Deni , Fischer Mirjam M. , Bos Henny M. W.
Published: May 2022

Same-sex parents face substantial stressors due to their sexual orientation, such as experiences of prejudice and prohibitive legal environments. This added stress is likely to lead to reduced physical and mental health in same-sex parents that, in turn, may translate into problematic behavioral outcomes in their children. To date, there are only a few nationally representative studies that investigate the well-being of children with same-sex parents. The current study takes a closer look at children’s behavioral outcomes, reported by a parent, using an adapted version of the emotional, conduct, hyperactivity, pro-social, and peer problems subscales of the Strengths and Difficulties Questionnaire (SDQ). We take advantage of unique data from the Netherlands based on a probability sample from population registers, whereby findings can be inferred to same-sex and different-sex parent households with parents between the ages of 30 and 65, and with children between the ages of 6 and 16 years (62 children with same-sex, and 72 children with different-sex parents). The findings obtained by coarsened exact matching suggest no significant disadvantages for children with same-sex parents compared to different-sex parents. We contextualize these findings in their wider cultural context, and recommend a renewed focus in future research away from deficit-driven comparisons.
Validity and Reliability of a Wearable Fitness Technology Scale in Portugal
Authors: Pedragosa Vera , Angosto Salvador , Gonçalves Celina
Published: May 2022

Currently, the use of technological devices for monitoring physical activity and in other mobile applications is widespread among sports users and is continuously growing. The aim of this study was the validation of the reliability of the use of Wearable Fitness Technology (WFT) in the context of fitness through quantitative analysis. Data collection was conducted online during the COVID-19 pandemic period. The sample consisted of 177 members of fitness centres who used MYZONE technology in Portugal. An Exploratory Factor Analysis (EFA) (Factor v10) and a Confirmatory Factor Analysis (CFA) (AMOS v22.0) were used to test the item fit. The results showed adequate fits, identifying a total of 14 items in a single factor to assess WFT in MYZONE users in fitness centres. The reliability of the scale showed adequate indices within the indicated limits. This study extends the current literature on WFT; provides information for providers, managers, and members; and aims to improve the fitness experience by developing a valid and reliable tool to assess the characteristics and incidence of WFT in fitness centres. The complexity of the WFT will affect the degree of user engagement with the fitness centre, highlighting the importance of using staff skills to generate motivational and innovative challenges to improve the service experience. Furthermore, this scale could be used to examine the influences of WFT on managers’ and members’ perceptions of the service experience in the Portuguese context.
The Potential for Bias across GPS-Accelerometer Combined Wear Criteria among Adolescents
Authors: Kepper Maura M. , Staiano Amanda E. , Broyles Stephanie T.
Published: May 2022

Physical activity has many health benefits, yet a large portion of our population is not meeting recommendations. Using accelerometry and global positioning systems (GPS) to accurately measure where people are active and to identify barriers and facilitators of activity across various settings will inform evidence-based policies and interventions to improve activity levels. Criteria for sufficient accelerometry data (e.g., number of days, minimum hours in a day) to accurately monitor free-living physical activity in adults and children have been widely studied, implemented, and reported by researchers. However, few best practice recommendations for researchers using GPS have been established. Therefore, this paper examined the impact of three co-wear criteria of varying stringency among a sample of children aged 10 to 16 years in Baton Rouge, Louisiana. Overall and location-based physical activity was consistent across the samples even within sociodemographic subgroups. Despite the lack of significant subgroup-specific mean differences in physical activity across the three samples, associations between sociodemographics and weight status and physical activity were significantly different depending on the device time-matching “co-wear” criteria applied. These differences demonstrate the critical impact co-wear criteria may have on conclusions drawn from research examining health disparities. There is a need for additional research and understanding of ideal co-wear criteria that reduce bias and accurately estimate free-living location-based physical activity across diverse populations.
The Resilience Scale in Portuguese Adults under Assisted Reproductive Techniques
Authors: Romeiro Joana , Nogueira Paulo , Hall Jenny , Caldeira Sílvia
Published: May 2022

Assessing resilience response to an adverse event such as infertility requires measuring the same construct adequately and accurately by nurses. The objective of this study was to explore the validity and reliability of the Portuguese version of the Resilience Scale for adults. A cross-sectional and methodological design was used, and factor analyses were performed. The sample comprised 140 adult Portuguese individuals under fertility treatment recruited from health-related websites. The findings yielded a bad fit of the hypothesized Resilience Scale structure with the observed data. Instead, a 21-item tool with a four-factor structure revealed high internal consistency (0.94). The tool correlated positively and significantly to the Portuguese version of the Spiritual Well-Being Questionnaire and was negatively associated and lacked correlation with the Fertility Adjustment Scale. The 21-item Resilience Scale is a reliable tool suitable to measure resilience in Portuguese adults under assisted reproductive techniques. This tool offers the opportunity of early recognition by health professionals aiming to enhance patients’ coping skills effectively and promote positive psychological and mental health outcomes.
Persistent Symptoms among Frontline Health Workers Post-Acute COVID-19 Infection
Authors: Wose Kinge Constance , Hanekom Susan , Lupton-Smith Alison , Akpan Francis , Mothibi Eula , Maotoe Thapelo , Lebatie Floyd , Majuba Pappie , Sanne Ian , Chasela Charles
Published: May 2022

Growing evidence shows that a significant number of patients with COVID-19 experience persistent symptoms, also known as long COVID-19. We sought to identify persistent symptoms of COVID-19 in frontline workers at Right to Care South Africa, who are past the acute phase of illness, using a cross-sectional survey. We analysed data from 207 eligible COVID-19 positive frontline workers who participated in a two-month post-COVID-19 online self-administered survey. The survey response rate was 30%; of the 62 respondents with a median age of 33.5 years (IQR= 30–44 years), 47 (76%) were females. The majority (n = 55; 88.7%) self-isolated and 7 (11.3%) were admitted to hospital at the time of diagnosis. The most common comorbid condition reported was hypertension, particularly among workers aged 45–55 years. The most reported persistent symptoms were characterised by fatigue, anxiety, difficulty sleeping, chest pain, muscle pain, and brain fog. Long COVID-19 is a serious phenomenon, of which much is still unknown, including its causes, how common it is especially in non-hospitalised healthcare workers, and how to treat it. Given the rise in COVID-19 cases, the prevalence of long COVID-19 is likely to be substantial; thus, the need for rehabilitation programs targeted at each persistent COVID-19 symptom is critical.
Factors Influencing Work Ability among the Working-Age Population in Singburi Province, Thailand
Authors: Kaewdok Teeraphun , Norkaew Saowanee , Sirisawasd Sanpatchaya , Choochouy Nattagorn
Published: May 2022

The ability to work is an essential factor in the quality of work life. This study aimed to determine factors related to work ability among the working population in Sing Buri Province. A total of 360 workers completed a cross-sectional survey using a questionnaire. The questionnaire included demographic data and work conditions, psychosocial factors, and measures of the seven components of the work-ability index (WAI). Mean, percentage, standard deviation, and stepwise multiple regression analyses were conducted to determine the rates and predictors of the WAI of the participants. Of the 360 participants, 61.40% were women with an average age of 43.00 ± 11.30 years; 36.70% had completed high school education. Their average work experience was 11.30 ± 8.50 years. The results revealed that the average WAI was 31.40 ± 4.15. 76.10% of the participants qualified for the moderate work ability index. The number of current diseases, age, and monthly income were found to significantly contribute to the prediction of the WAI (p < 0.05). This finding suggests that the relevant agencies should support a policy, project or program related to health promotion among the working population with physical health conditions. Promoting occupations should be considered to raise income policy. In doing so, work ability among the working population may be improved.
Assessment of Infection Prevention and Control Measures at Points of Entry in Sierra Leone in 2021: A Cross-Sectional Study
Authors: Kamara Kadijatu Nabie , Squire James Sylvester , Kanu Joseph Sam , Carshon-Marsh Ronald , Koroma Zikan , Koroma Aminata Tigiedankay , Maruta Anna , Kallon Christiana , Manzi Marcel , Camara Bienvenu Salim , Sargsyan Aelita , Delamou Alexandre , Guth Jamie Ann , Reid Anthony , Khogali Mohamed Ahmed , Vandi Mohamed Alex
Published: May 2022

Implementing and monitoring infection prevention and control (IPC) measures at immigration points of entry (PoEs) is key to preventing infections, reducing excessive use of antimicrobials, and tackling antimicrobial resistance (AMR). Sierra Leone has been implementing IPC measures at four PoEs (Queen Elizabeth II Quay port, Lungi International Airport, and the Jendema and Gbalamuya ground crossings) since the last Ebola outbreak in 2014–2015. We adapted the World Health Organization IPC Assessment Framework tool to assess these measures and identify any gaps in their components at each PoE through a cross-sectional study in May 2021. IPC measures were Inadequate (0–25%) at Queen Elizabeth II Quay port (21%; 11/53) and Jendema (25%; 13/53) and Basic (26–50%) at Lungi International Airport (40%; 21/53) and Gbalamuya (49%; 26/53). IPC components with the highest scores were: having a referral system (85%; 17/20), cleaning and sanitation (63%; 15/24), and having a screening station (59%; 19/32). The lowest scores (0% each) were reported for the availability of IPC guidelines and monitoring of IPC practices. This was the first study in Sierra Leone highlighting significant gaps in the implementation of IPC measures at PoEs. We call on the AMR multisectoral coordinating committee to enhance IPC measures at all PoEs.
A Path Model of the Relationship between Mood, Exercise Behavior, Coping, and Mental Health among Malaysians during the COVID-19 Pandemic
Authors: Yew Jing Mun , Kueh Yee Cheng , Norsa’adah Bachok , Leong Foo Weng , Tang Heen Yeong , Kuan Garry
Published: May 2022

The purpose of this study was to examine the relationship between the mood, physical activity, coping, and mental health of Malaysians during the COVID-19 pandemic. A cross-sectional study was conducted via an online survey, with self-administered questionnaires. The respondents were recruited using snowball sampling techniques. The Brunel Mood Scale (BRUMS), the Exercise Regulations in Exercise-3 (BREQ-3), the Brief Coping Orientation of Problem Experienced (Brief COPE), and the Depression, Anxiety, and Stress Scale (DASS-21) were used. A path analysis was conducted on the data. A total of 842 people participated in the survey. The mean age of participants was 22 years (interquartile range = 6) and 24.0% were male. The final path model fitted the data well, with a comparative fit index of 0.998, a Tucker–Lewis index of 0.988, a standardized root mean square residual of 0.001, and a root mean square error of approximation of 0.072. In this study, there were significant path relationships between mood, exercise behavior, coping, and mental health. Additionally, it was demonstrated that the variables of mood, exercise behavior, and coping have both direct and indirect effects on mental health. The results also suggested that utilizing appropriate coping skills, exercise behavior, and positive mood can directly lower levels of depression, anxiety and stress, and that appropriate coping skills and positive mood can directly affect exercise behavior.
Cardiovascular Mortality and Leaded Aviation Fuel: Evidence from Piston-Engine Air Traffic in North Carolina
Authors: Klemick Heather , Guignet Dennis , Bui Linda T. , Shadbegian Ron , Milani Cameron
Published: May 2022

Leaded fuel used by piston-engine aircraft is the largest source of airborne lead emissions in the United States. Previous studies have found higher blood lead levels in children living near airports where leaded aviation fuel is used. However, little is known about the health effects on adults. This study is the first to examine the association between exposure to aircraft operations that use leaded aviation fuel and adult cardiovascular mortality. We estimated the association between annual piston-engine air traffic and cardiovascular mortality among adults age 65 and older near 40 North Carolina airports during 2000 to 2017. We used several strategies to minimize the potential for bias due to omitted variables and confounding from other health hazards at airports, including coarsened exact matching, location-specific intercepts, and adjustment for jet-engine and other air traffic that does not use leaded fuel. Our findings are mixed but suggestive of adverse effects. We found higher rates of cardiovascular mortality within a few kilometers downwind of single- and multi-runway airports, though these results are not always statistically significant. We also found significantly higher cardiovascular mortality rates within a few kilometers and downwind of single-runway airports in years with more piston-engine air traffic. We did not consistently find a statistically significant association between cardiovascular mortality rates and piston-engine air traffic near multi-runway airports, where there was greater uncertainty in our measure of the distance between populations and aviation exposures. These results suggest that (i) reducing lead emissions from aviation could yield health benefits for adults, and (ii) more refined data are needed to obtain more precise estimates of these benefits. Subject Areas: Toxic Substances, Health, Epidemiology, Air Pollution, Ambient Air Quality. JEL codes: Q53, I18.
Determinants of COVID-19 Vaccine Uptake among Female Foreign Domestic Workers in Hong Kong: A Cross-Sectional Quantitative Survey
Authors: Sumerlin Timothy S. , Kim Jean H. , Wang Zixin , Hui Alvin Yik-Kiu , Chung Roger Y.
Published: May 2022

Globally, minority groups and non-citizens may not be sufficiently included in the COVID-19 vaccine coverage. This study seeks to understand determinants of vaccine uptake among female foreign domestic workers (FDWs) in Hong Kong. We conducted a cross-sectional study of female FDWs (n = 581) from June to August 2021. Respondents completed an online survey obtaining sociodemographic, employment, and health status information. Based upon the socio-ecological model, we obtained individual, interpersonal, and socio-structural factors that may be associated with COVID-19 vaccine uptake. Multivariable logistic regression analysis was used to examine factors associated with having received at least one dose of a COVID-19 vaccine. At the individual level, agreeing that taking COVID-19 vaccines can contribute to COVID-19 control in Hong Kong (OR 6.11, 95% CI 2.27–16.43) was associated with increased vaccine uptake, while being worried of severe side-effects from vaccination (OR 0.29, 95% CI 0.16–0.55) was associated with decreased uptake. At the interpersonal level, those being encouraged by their employer (OR 2.05, 95% CI 1.06–3.95) and family members (OR 2.27, 95% CI 1.17–4.38) were more likely to be vaccinated, while at the socio-structural level, believing vaccination would violate religious beliefs (OR 0.19, 95% CI 0.06–0.65) was associated with decreased uptake. The government can formulate a multi-level approach according to our findings to target the remaining unvaccinated FDW population.
A Rapid Review of Interventions to Increase Hepatitis B Testing, Treatment, and Monitoring among Migrants Living in Australia
Authors: Rajkumar Vishnupriya , McCausland Kahlia , Lobo Roanna
Published: May 2022

Chronic hepatitis B (CHB) disproportionately affects migrants with low health literacy and help-seeking behaviour living in high-income countries. Evidence of effective interventions is required to increase hepatitis B (HBV) testing, treatment, and monitoring. Available evidence from Medline, Embase, Scopus, Google, and Google Scholar was identified, collated, and synthesised. Inclusion criteria included grey and peer-reviewed literature published in English between January 2012 and December 2021. Systematic reviews and meta-analyses were excluded. Seventeen peer-reviewed articles met the inclusion criteria. Most interventions were conducted at the individual level and were typically outreach testing initiatives. One study was conducted at a structural level. All studies were successful in encouraging HBV screening uptake, and 10 studies demonstrated effective linkage to care. Two studies showed evidence of monitoring participants post-intervention. Most interventions had more female than male participants. Interventions conducted across community and clinical-based settings had more participants engage in screening and/or linkage to care in community settings. Effective interventions to prevent HBV transmission and CHB-related morbidity and mortality were approaches that utilised linguistic-specific and culturally appropriate resources to successfully engage migrants. Community outreach programmes that educate participants about HBV transmission, screening, and treatment can promote community dialogue and understanding to reduce stigma and discrimination.
Transition from Nurses to Medicalized Elderly Caregivers: Comparison on Willingness between Traditional and Modern Regions in China
Authors: Gao Wenqing , Li Shuailong , Chi Zhuoyuan , Gong Fangfang , Tang Wenxi
Published: May 2022

As China is transitioning to an aging society, the Chinese government has proposed an eldercare pattern, called medicalized elderly care, to help solve the rapid aging and health care problems together. However, the shortage of elderly caregivers is a critical issue, with deficiency both in quantity and quality. This study aims to survey nurses’ willingness to transition into medicalized elderly caregivers and compare it between modern and traditional regions. Nurses working in Guangdong (modern region) and Jilin (traditional region) were investigated using a self-administered questionnaire in October 2021. We analyzed the influencing factors through χ²-test, t-test a and binary logistic regression model and further explored the influence of region using propensity score matching (PSM). A total of 1227 nurses were included, with 726 (59.2%) of them showing willingness to transition. Nurses from traditional regions showed a significantly higher willingness to transition after PSM (p = 0.027). Other factors influencing nurses’ willingness were age, education, lived with older adults, participated in voluntary activities related to older adults, visited eldercare institutions, attitudes toward older adults, knowledge about older adults, hospice care attitudes and death attitudes. The willingness of nurses to transition was not high enough. To have more willing and skillful human resources for eldercare, we need a more “intimate society for older adults” in the first place.
Generational Differences, Risk Tolerance, and Ownership of Financial Securities: Evidence from the United States
Authors: Antwi Johnson , Naanwaab Cephas B.
Published: May 2022

This paper examines the relationship between generational differences, risk tolerance, and attitudes towards financial investments in a nationally representative sample from the United States of America. The sample consists of pooled cross-sectional data of three waves (2012–2018) and 80,000 observations from the National Financial Capability Study (NFCS). Using a probit model (with and without sample selection), all of the predictor variables are estimated to have statistically significant effects on the ownership of financial securities, with the expected sign effects. There is clearly a generational cohort effect, whereby Baby Boomers are on average more likely to own financial investments than Millennials, controlling for other factors such as incomes, education, and financial literacy. Generation Xers are statistically less likely to have investments in financial securities compared to Millennials. In general, Baby Boomers are more risk-averse and Generation Xers are more risk-loving than Millennials, accounting for education and income levels. The paper reveals a conundrum in which Baby Boomers (Gen Xers), although more (less) risk-averse, are more (less) likely to own financial securities. We control for reverse causality (endogeneity) in the relationship between risk tolerance and the ownership of securities, using the bivariate probit model. The level of financial knowledge of respondents correlates highly with asset ownership: individuals with high and medium levels of financial knowledge are more likely to own financial assets than those with low levels of financial knowledge. To address the limitations of the current findings with regard to generational attitudes towards financial investments, further research is recommended.
Transgenic Mouse Models of Alzheimer’s Disease: An Integrative Analysis
Authors: Sanchez-Varo Raquel , Mejias-Ortega Marina , Fernandez-Valenzuela Juan Jose , Nuñez-Diaz Cristina , Caceres-Palomo Laura , Vegas-Gomez Laura , Sanchez-Mejias Elisabeth , Trujillo-Estrada Laura , Garcia-Leon Juan Antonio , Moreno-Gonzalez Ines , Vizuete Marisa , Vitorica Javier , Baglietto-Vargas David , Gutierrez Antonia
Published: May 2022

Alzheimer’s disease (AD) constitutes the most prominent form of dementia among elderly individuals worldwide. Disease modeling using murine transgenic mice was first initiated thanks to the discovery of heritable mutations in amyloid precursor protein (APP) and presenilins (PS) genes. However, due to the repeated failure of translational applications from animal models to human patients, along with the recent advances in genetic susceptibility and our current understanding on disease biology, these models have evolved over time in an attempt to better reproduce the complexity of this devastating disease and improve their applicability. In this review, we provide a comprehensive overview about the major pathological elements of human AD (plaques, tauopathy, synaptic damage, neuronal death, neuroinflammation and glial dysfunction), discussing the knowledge that available mouse models have provided about the mechanisms underlying human disease. Moreover, we highlight the pros and cons of current models, and the revolution offered by the concomitant use of transgenic mice and omics technologies that may lead to a more rapid improvement of the present modeling battery.
Insights into the Pharmacokinetics and In Vitro Cell-Based Studies of the Imidazoline I2 Receptor Ligand B06
Authors: Bagán Andrea , Morales-García José A. , Griñán-Ferré Christian , Díaz Caridad , Pérez del Palacio José , Ramos Maria C. , Vicente Francisca , Pérez Belén , Brea José , Loza María Isabel , Pallàs Mercè , Escolano Carmen
Published: May 2022

The impact of neurodegenerative diseases (ND) is becoming unbearable for humankind due to their vast prevalence and the lack of efficacious treatments. In this scenario, we focused on imidazoline I2 receptors (I2-IR) that are widely distributed in the brain and are altered in patients with brain disorders. We took the challenge of modulating I2-IR by developing structurally new molecules, in particular, a family of bicyclic α-iminophosphonates, endowed with high affinity and selectivity to these receptors. Treatment of two murine models, one for age-related cognitive decline and the other for Alzheimer’s disease (AD), with representative compound B06 ameliorated their cognitive impairment and improved their behavioural condition. Furthermore, B06 revealed beneficial in vitro ADME-Tox properties. The pharmacokinetics (PK) and metabolic profile are reported to de-risk B06 for progressing in the preclinical development. To further characterize the pharmacological properties of B06, we assessed its neuroprotective properties and beneficial effect in an in vitro model of Parkinson’s disease (PD). B06 rescued the human dopaminergic cell line SH-SY5Y from death after treatment with 6-hydroxydopamine (6-OHDA) and showed a crucial anti-inflammatory effect in a cellular model of neuroinflammation. This research reveals B06 as a putative candidate for advancing in the difficult path of drug discovery and supports the modulation of I2-IR as a fresh approach for the therapy of ND.
Photoprotection Differences between Dominant Tree Species at Mid- and Late-Successional Stages in Subtropical Forests in Different Seasonal Environments
Authors: Lin Wei , Yu Zhengchao , Luo Yanna , He Wei , Yan Guanzhao , Peng Changlian
Published: May 2022

Plants growing in subtropical regions are often affected by high temperature and high light in summer and low temperature and high light in winter. However, few studies have compared the photoprotection mechanism of tree species at different successional stages in these two environments, although such studies would be helpful in understanding the succession of forest communities in subtropical forests. In order to explore the strategies used by dominant species at different successional stages to cope with these two environmental conditions, we selected two dominant species in the mid-successional stage, Schima superba and Castanopsis chinensis, and two dominant species in the late-successional stage, Machilus chinensis and Cryptocarya chinensis. The cell membrane permeability, chlorophyll fluorescence, chlorophyll content, and a few light-protective substances of these dominant species were measured in summer and winter. The results show that in summer, the young leaves of dominant species in the mid-successional stage showed higher anthocyanin content and superoxide dismutase (SOD) activity, while those in the late-successional stage showed higher flavonoid and total phenolic content, total antioxidant activity, non-photochemical quenching (NPQ), and carotenoid/chlorophyll (Car/Chl) ratio. In winter, young leaves of dominant species in the mid-successional stage were superior to those in the late-successional stage only in terms of catalase (CAT) activity and NPQ, while the anthocyanin, flavonoids, and total phenol content, total antioxidant capacity, and Car/Chl ratio were significantly lower compared to the late-successional stage. Our results show that the dominant species in different successional stages adapted to environmental changes in different seasons through the alterations in their photoprotection strategies. In summer, the dominant species in the mid-successional stage mainly achieved photoprotection through light shielding and reactive-oxygen-species scavenging by SOD, while the antioxidant capacity of trees in the late-successional stage mainly came from an increased antioxidative compounds and heat dissipation. In winter, the dominant species in the mid-successional stage maintained their photoprotective ability mainly through the scavenging of reactive oxygen species by CAT and the heat dissipation provided by NPQ, while those in the late-successional stage were mainly protected by a combination of processes, including light shielding, heat dissipation, and antioxidant effects provided by enzymatic and non-enzymatic antioxidant systems. In conclusion, our study partially explains the mechanism of community succession in subtropical forests.
Green Synthesis of Silymarin–Chitosan Nanoparticles as a New Nano Formulation with Enhanced Anti-Fibrotic Effects against Liver Fibrosis
Authors: Abdullah Abdullah Saad , Sayed Ibrahim El Tantawy El , El-Torgoman Abdel Moneim A. , Kalam Abul , Wageh S. , Kamel Maher A.
Published: May 2022

Background: Silymarin (SIL) has long been utilized to treat a variety of liver illnesses, but due to its poor water solubility and low membrane permeability, it has a low oral bioavailability, limiting its therapeutic potential. Aim: Design and evaluate hepatic-targeted delivery of safe biocompatible formulated SIL-loaded chitosan nanoparticles (SCNPs) to enhance SIL’s anti-fibrotic effectiveness in rats with CCl4-induced liver fibrosis. Methods: The SCNPs and chitosan nanoparticles (CNPs) were prepared by ionotropic gelation technique and are characterized by physicochemical parameters such as particle size, morphology, zeta potential, and in vitro release studies. The therapeutic efficacy of successfully formulated SCNPs and CNPs were subjected to in vivo evaluation studies. Rats were daily administered SIL, SCNPs, and CNPs orally for 30 days. Results: The in vivo study revealed that the synthesized SCNPs demonstrated a significant antifibrotic therapeutic action against CCl4-induced hepatic injury in rats when compared to treated groups of SIL and CNPs. SCNP-treated rats had a healthy body weight, with normal values for liver weight and liver index, as well as significant improvements in liver functions, inflammatory indicators, antioxidant pathway activation, and lipid peroxidation reduction. The antifibrotic activities of SCNPs were mediated by suppressing the expression of the main fibrosis mediators TGFβR1, COL3A1, and TGFβR2 by boosting the hepatic expression of protective miRNAs; miR-22, miR-29c, and miR-219a, respectively. The anti-fibrotic effects of SCNPs were supported by histopathology and immunohistochemistry (IHC) study. Conclusions: According to the above results, SCNPs might be the best suitable carrier to target liver cells in the treatment of liver fibrosis.
The Effect of Gap Distance between a Pin and Water Surface on the Inactivation of Escherichia coli Using a Pin-to-Water Plasma
Authors: Lim Junghyun , Hong Eun Jeong , Kim Seong Bong , Ryu Seungmin
Published: May 2022

Atmospheric plasmas have been applied for the inactivation of microorganisms. Industrials demand to investigate the relation of the key reactive species induced by plasmas and the operating parameters including boundary conditions in order to control plasma treatment processes. In this study, we investigated the effect of gap distance between a pin-electrode and water surface on inactivation efficacy. When the gap distance decreased from 5 mm to 1 mm, the reduction of Escherichia coli (E. coli) was increased to more than 4 log CFU/mL. The reactive oxygen species measured optically and spectrophotometrically were influenced by gap distance. The results from electron spin resonance (ESR) analysis showed that the pin-to-water plasma generated hydroxyl radical (OH•) and singlet oxygen (1O2) in the water and superoxide radical (O2−•) served as a precursor of OH•. The inactivation of E. coli was significantly alleviated by sodium azide (1O2 scavenger), indicating that 1O2 contributes the most to bacterial inactivation. These findings provide a potentially effective strategy for bacterial inactivation using a pin-to-water plasma.
Molecular Research and Treatment of Skin Diseases
Authors: Koller Ulrich , Bauer Johann W.
Published: May 2022

The intention of this Special Issue is to highlight current treatment options to target the cause, as well as disease-associated complications, of skin diseases, including a group of monogenetic skin disorders referred to as genodermatoses [...]
Increasing Heavy Metal Tolerance by the Exogenous Application of Organic Acids
Authors: Vega Andrea , Delgado Ninoska , Handford Michael
Published: May 2022

Several metals belong to a group of non-biodegradable inorganic constituents that, at low concentrations, play fundamental roles as essential micronutrients for the growth and development of plants. However, in high concentrations they can have toxic and/or mutagenic effects, which can be counteracted by natural chemical compounds called chelators. Chelators have a diversity of chemical structures; many are organic acids, including carboxylic acids and cyclic phenolic acids. The exogenous application of such compounds is a non-genetic approach, which is proving to be a successful strategy to reduce damage caused by heavy metal toxicity. In this review, we will present the latest literature on the exogenous addition of both carboxylic acids, including the Kreb’s Cycle intermediates citric and malic acid, as well as oxalic acid, lipoic acid, and phenolic acids (gallic and caffeic acid). The use of two non-traditional organic acids, the phytohormones jasmonic and salicylic acids, is also discussed. We place particular emphasis on physiological and molecular responses, and their impact in increasing heavy metal tolerance, especially in crop species.
Prof. Ludger Wessjohann: A Lifelong Career Dedicated to a Remarkable Service in “Natural Products Sciences”
Authors: Hussain Hidayat
Published: May 2022

It is a great honor and a pleasure for me to serve as Guest Editor for this Issue of the “International Journal of Molecular Sciences”, dedicated to our mentor and colleague, Professor Dr [...]
New Beta-lactamases in Candidate Phyla Radiation: Owning Pleiotropic Enzymes Is a Smart Paradigm for Microorganisms with a Reduced Genome
Authors: Maatouk Mohamad , Ibrahim Ahmad , Pinault Lucile , Armstrong Nicholas , Azza Said , Rolain Jean-Marc , Bittar Fadi , Raoult Didier
Published: May 2022

The increased exploitation of microbial sequencing methods has shed light on the high diversity of new microorganisms named Candidate Phyla Radiation (CPR). CPR are mainly detected via 16S rRNA/metabarcoding analyses or metagenomics and are found to be abundant in all environments and present in different human microbiomes. These microbes, characterized by their symbiotic/epiparasitic lifestyle with bacteria, are directly exposed to competition with other microorganisms sharing the same ecological niche. Recently, a rich repertoire of enzymes with antibiotic resistance activity has been found in CPR genomes by using an in silico adapted screening strategy. This reservoir has shown a high prevalence of putative beta-lactamase-encoding genes. We expressed and purified five putative beta-lactamase sequences having the essential domains and functional motifs from class A and class B beta-lactamase. Their enzymatic activities were tested against various beta-lactam substrates using liquid chromatography-mass spectrometry (LC-MS) and showed some beta-lactamase activity even in the presence of a beta-lactamase inhibitor. In addition, ribonuclease activity was demonstrated against RNA that was not inhibited by sulbactam and EDTA. None of these proteins could degrade single- and double-stranded-DNA. This study is the first to express and test putative CPR beta-lactamase protein sequences in vitro. Our findings highlight that the reduced genomes of CPR members harbor sequences encoding for beta-lactamases known to be multifunction hydrolase enzymes.
Immune Checkpoint Blockade via PD-L1 Potentiates More CD28-Based than 4-1BB-Based Anti-Carbonic Anhydrase IX Chimeric Antigen Receptor T Cells
Authors: de Campos Najla Santos Pacheco , de Oliveira Beserra Adriano , Pereira Pedro Henrique Barbosa , Chaves Alexandre Silva , Fonseca Fernando Luiz Affonso , da Silva Medina Tiago , dos Santos Tiago Goss , Wang Yufei , Marasco Wayne Anthony , Suarez Eloah Rabello
Published: May 2022

The complete regression of clear cell renal cell carcinoma (ccRCC) obtained pre-clinically with anti-carbonic anhydrase IX (CAIX) G36 chimeric antigen receptor (CAR) T cells in doses equivalent to ≅108 CAR T cells/kg renewed the potential of this target to treat ccRCC and other tumors in hypoxia. The immune checkpoint blockade (ICB) brought durable clinical responses in advanced ccRCC and other tumors. Here, we tested CD8α/4-1BB compared to CD28-based anti-CAIX CAR peripheral blood mononuclear cells (PBMCs) releasing anti-programmed cell death ligand-1 (PD-L1) IgG4 for human ccRCC treatment in vitro and in an orthotopic NSG mice model in vivo. Using a ≅107 CAR PBMCs cells/kg dose, anti-CAIX CD28 CAR T cells releasing anti-PD-L1 IgG highly decrease both tumor volume and weight in vivo, avoiding the occurrence of metastasis. This antitumoral superiority of CD28-based CAR PBMCs cells compared to 4-1BB occurred under ICB via PD-L1. Furthermore, the T cell exhaustion status in peripheral CD4 T cells, additionally to CD8, was critical for CAR T cells efficiency. The lack of hepatotoxicity and nephrotoxicity upon the administration of a 107 CAR PMBCs cells/kg dose is the basis for carrying out clinical trials using anti-CAIX CD28 CAR PBMCs cells releasing anti-PD-L1 antibodies or anti-CAIX 4-1BB CAR T cells, offering exciting new prospects for the treatment of refractory ccRCC and hypoxic tumors.