bluuebunny/crossref_metadata_2025 huggingface dataset:
A Macroeconomic SIR Model for COVID-19
Authors: Bayraktar Erhan , Cohen Asaf , Nellis April
Published: August 2021

The COVID-19 pandemic and subsequent lockdowns highlight the close and delicate relationship between a country’s public health and economic health. Models that combine macroeconomic factors with traditional epidemic dynamics to calculate the impacts of a disease outbreak are therefore extremely useful for policymakers seeking to evaluate the best course of action in such a crisis. We developed a macroeconomic SIR model that considers herd immunity, behavior-dependent transmission rates, remote workers, and the indirect externalities of lockdowns. It is formulated as an exit time control problem where a social planner is able to prescribe separate levels of the lockdown low-risk and high-risk portions of the adult population. The model predicts that by considering the possibility of reaching herd immunity, high-risk individuals are able to leave lockdown sooner than in models where herd immunity is not considered. Additionally, a behavior-dependent transmission rate (which represents increased personal caution in response to increased infection levels) can lower both output loss and total mortality. Overall, the model-determined optimal lockdown strategy, combined with individual actions to slow virus transmission, is able to reduce total mortality to one-third of the model-predicted no-lockdown level of mortality.
Application of the Gadidae Fish Processing Waste for Food Grade Gelatin Production
Authors: Zarubin Nikita Yu. , Kharenko Elena N. , Bredikhina Olga V. , Arkhipov Leonid O. , Zolotarev Konstantin V. , Mikhailov Anton N. , Nakhod Valeriya I. , Mikhailova Marina V.
Published: August 2021

Waste from fish cutting (heads, swim bladders, fins, skin, and bones) is a high-value technological raw material for obtaining substances and products with a wide range of properties. The possibility of using waste from cutting fish of the Gadidae family: the Alaska pollock (Gadus chalcogrammus) and the Pacific cod (Gadus macrocephalus), processed in the coastal zone, is scientifically substantiated. In this work, a technology has been developed for processing accumulated waste from fish cutting in order to obtain fish gelatin, which is characterized by high protein content (more than 80.0%) and a full set of essential and nonessential amino acids. We studied the quality of fish gelatin obtained from wastes from cutting the fish of the Gadidae family. The possibility of using fish gelatin as a component of fish products is shown; the dose of its introduction into the fish products is substantiated. The data obtained made it possible to recommend the use of fish processing waste products as a gelling component and a source of amino acids in multicomponent food systems.
Do Mass Spectrometry-Derived Metabolomics Improve the Prediction of Pregnancy-Related Disorders? Findings from a UK Birth Cohort with Independent Validation
Authors: McBride Nancy , Yousefi Paul , Sovio Ulla , Taylor Kurt , Vafai Yassaman , Yang Tiffany , Hou Bo , Suderman Matthew , Relton Caroline , Smith Gordon C. S. , Lawlor Deborah A.
Published: August 2021

Many women who experience gestational diabetes (GDM), gestational hypertension (GHT), pre-eclampsia (PE), have a spontaneous preterm birth (sPTB) or have an offspring born small/large for gestational age (SGA/LGA) do not meet the criteria for high-risk pregnancies based upon certain maternal risk factors. Tools that better predict these outcomes are needed to tailor antenatal care to risk. Recent studies have suggested that metabolomics may improve the prediction of these pregnancy-related disorders. These have largely been based on targeted platforms or focused on a single pregnancy outcome. The aim of this study was to assess the predictive ability of an untargeted platform of over 700 metabolites to predict the above pregnancy-related disorders in two cohorts. We used data collected from women in the Born in Bradford study (BiB; two sub-samples, n = 2000 and n = 1000) and the Pregnancy Outcome Prediction study (POPs; n = 827) to train, test and validate prediction models for GDM, PE, GHT, SGA, LGA and sPTB. We compared the predictive performance of three models: (1) risk factors (maternal age, pregnancy smoking, BMI, ethnicity and parity) (2) mass spectrometry (MS)-derived metabolites (n = 718 quantified metabolites, collected at 26–28 weeks’ gestation) and (3) combined risk factors and metabolites. We used BiB for the training and testing of the models and POPs for independent validation. In both cohorts, discrimination for GDM, PE, LGA and SGA improved with the addition of metabolites to the risk factor model. The models’ area under the curve (AUC) were similar for both cohorts, with good discrimination for GDM (AUC (95% CI) BiB 0.76 (0.71, 0.81) and POPs 0.76 (0.72, 0.81)) and LGA (BiB 0.86 (0.80, 0.91) and POPs 0.76 (0.60, 0.92)). Discrimination was improved for the combined models (compared to the risk factors models) for PE and SGA, with modest discrimination in both studies (PE-BiB 0.68 (0.58, 0.78) and POPs 0.66 (0.60, 0.71); SGA-BiB 0.68 (0.63, 0.74) and POPs 0.64 (0.59, 0.69)). Prediction for sPTB was poor in BiB and POPs for all models. In BiB, calibration for the combined models was good for GDM, LGA and SGA. Retained predictors include 4-hydroxyglutamate for GDM, LGA and PE and glycerol for GDM and PE. MS-derived metabolomics combined with maternal risk factors improves the prediction of GDM, PE, LGA and SGA, with good discrimination for GDM and LGA. Validation across two very different cohorts supports further investigation on whether the metabolites reflect novel causal paths to GDM and LGA.
Study on Manufacturing Technology of Ag-8.5Au-3.5Pd Fine Alloy Wire
Authors: Cao Jun , Zhang Junchao , Wu Baoan , Tang Huiyi , Lv Changchun , Song Kexing , Yang Guannan , Cui Chengqiang , Gao Yangguang
Published: August 2021

The performance of Ag-8.5Au-3.5Pd alloy wire after cold deformation and annealing were analyzed by SEM (scanning electron microscope), strength tester and resistivity tester. The processing process and performance change characteristics of Ag-8.5Au-3.5Pd alloy wire were studied. The results show that alloy wire grains gradually form a fibrous structure along with the increase in deformation. The strength of the wire increases with the increase in deformation rate, but the increase trend becomes flat once the deformation rate is higher than 92.78%; the resistivity of Ag-8.5Au-3.5Pd alloy wire decreases with the increase in annealing temperature, reaching minimum (2.395 × 10−8 Ω·m) when the annealing temperature is 500 °C; the strength of Ag-8.5Au-3.5Pd alloy wire decreases with the increase in annealing temperature. When the annealing temperature is 500 °C, the strength and elongation of the φ0.2070 mm Ag-8.5Au-3.5Pd alloy wire are 287 MPa and 25.7%, respectively; the fracture force and elongation of φ0.020 mm Ag-8.5Au-3.5Pd alloy wire are 0.0876 N and 14.8%, respectively. When the annealing temperature is 550 °C, the metal grains begin to grow and the mechanical performance decrease; the φ0.020 mm Ag-8.5Au-3.5Pd alloy wire have good surface quality when the tension range is 2.5–3.0 g.
Design and Implementation of a Composite Hydrophone of Sound Pressure and Sound Pressure Gradient
Authors: Zhang Guojun , Zhang Lansheng , Ji Songxiang , Yang Xi , Wang Renxin , Zhang Wendong , Yang Shie
Published: August 2021

The bionic cilium MEMS vector hydrophone has the characteristics of low power consumption, small volume, and good low-frequency response. Nevertheless, there exists the problem of left–right ambiguity in the azimuth estimation of a single hydrophone. In order to solve the engineering application problem, a sound-pressure sound-pressure-gradient hydrophone is designed in this paper. The new composite hydrophone consists of two channels. The bionic cilium microstructure is optimized and used as the vector channel, to collect the sound pressure gradient information, and a scalar channel, based on a piezoelectric ceramic tube, is added, to receive the sound pressure information. The theoretical analysis, simulation analysis, and test analysis of the composite hydrophone are carried out, respectively. The test results show that the sensitivities of the hydrophone can reach up to −188 dB (vector channel) and −204 dB (scalar channel). The problem of left–right ambiguity is solved by combining the sound pressure and sound pressure gradient in different ways. This is of great significance in the engineering application of single cilium MEMS hydrophone orientation.
Visualization of Local Concentration and Viscosity Distribution during Glycerol-Water Mixing in a Y-Shape Minichannel: A Proof-of-Concept-Study
Authors: Medina Isabel , Deuerling Julian , Kumari Pooja , Scholl Stephan , Rädle Matthias
Published: August 2021

The work presents an efficient and non-invasive method to visualize the local concentration and viscosity distribution of two miscible and non-reacting substances with a significant viscosity difference in a microchannel with a Y-shape cell. The proof-of-concept setup consists of a near-infrared (NIR) camera and cost-effective dome lighting with NIR light-emitting diodes (LED) covering the wavelength range of 1050 to 1650 nm. Absorption differences of glycerol and water and their mixtures with a mass fraction of glycerol from 0 to 0.95 gGlycgtotal−1 were analyzed in the NIR spectral area. The resulting measurement images were converted in a concentration profile by using absorbance calculated with Lambert–Beer law. A linear behavior between the concentration and the absorption coefficient is demonstrated. The result of local concentration in mass fraction was used to determine the local viscosity and illustrated as distribution images. By variating the fluid parameters, the influences of the highly different original viscosities in the mixing procedure were investigated and visualized.
Selection of Bacterial Strains for Control of Root-Knot Disease Caused by Meloidogyne incognita
Authors: Migunova Varvara D. , Tomashevich Natalia S. , Konrat Alena N. , Lychagina Svetlana V. , Dubyaga Valentina M. , D’Addabbo Trifone , Sasanelli Nicola , Asaturova Anzhela M.
Published: August 2021

Root-knot disease caused by Meloidogyne incognita leads to significant crop yield losses that may be aggravated by the association with pathogenic fungi and bacteria. Biological agents can be effectively used against the complex disease of root-knot nematode and pathogenic fungi. In this study, 35 bacterial strains were analyzed for their in vitro nematicidal, antagonistic and growth stimulation activities. Based on results from the in vitro assays, grow-box experiments on tomato and cucumber were carried out with the strain BZR 86 of Bacillus velezensis applied at different concentrations. Effects of B. velezensis BZR 86 on the development of root-knot disease were evaluated by recording root gall index, number of galls and number of eggs in egg masses. Application of B. velezensis BZR 86 noticeably decreased the development of root-knot disease on tomato and cucumber plants, as well as significantly increased growth and biomass of cucumber plants in accordance with bacterial concentration. This study seems to demonstrate that strain B. velezensis BZR 86 could be an additional tool for an environmentally safe control of root-knot disease on horticultural crops.
West Nile Virus in the State of Ceará, Northeast Brazil
Authors: Löwen Levy Chalhoub Flávia , Maia de Queiroz-Júnior Eudson , Holanda Duarte Bruna , Eielson Pinheiro de Sá Marcos , Cerqueira Lima Pedro , Carneiro de Oliveira Ailton , Medeiros Neves Casseb Lívia , Leal das Chagas Liliane , Antônio de Oliveira Monteiro Hamilton , Sebastião Alberto Santos Neves Maycon , Facundo Chaves Cyro , Jean da Silva Moura Paulo , Machado Rapello do Nascimento Aline , Giesbrecht Pinheiro Rodrigo , Roberio Soares Vieira Antonio , Bergson Pinheiro Moura Francisco , Osvaldo Rodrigues da Silva Luiz , Nogueira Farias da Escóssia Kiliana , Caranha de Sousa Lindenberg , Leticia Cavalcante Ramalho Izabel , Williams Lopes da Silva Antônio , Maria Simōes Mello Leda , Felix de Souza Fábio , das Chagas Almeida Francisco , dos Santos Rodrigues Raí , do Vale Chagas Diego , Ferreira-de-Brito Anielly , Ribeiro Leite Jardim Cavalcante Karina , Angélica Monteiro de Mello Mares-Guia Maria , Martins Guerra Campos Vinícius , Rodrigues da Costa Faria Nieli , Adriano da Cunha e Silva Vieira Marcelo , Cesar Lima de Mendonça Marcos , Camila Amorim de Alvarenga Pivisan Nayara , de Oliveira Moreno Jarier , Aldessandra Diniz Vieira Maria , Gonçalves de Aguiar Gomes Ricristhi , Montenegro de Carvalho Araújo Fernanda , Henrique de Oliveira Passos Pedro , Garkauskas Ramos Daniel , Pecego Martins Romano Alessandro , Carício Martins Lívia , Lourenço-de-Oliveira Ricardo , Maria Bispo de Filippis Ana , Pauvolid-Corrêa Alex
Published: August 2021

In June 2019, a horse with neurological disorder was diagnosed with West Nile virus (WNV) in Boa Viagem, a municipality in the state of Ceará, northeast Brazil. A multi-institutional task force coordinated by the Brazilian Ministry of Health was deployed to the area for case investigation. A total of 513 biological samples from 78 humans, 157 domestic animals and 278 free-ranging wild birds, as well as 853 adult mosquitoes of 22 species were tested for WNV by highly specific serological and/or molecular tests. No active circulation of WNV was detected in vertebrates or mosquitoes by molecular methods. Previous exposure to WNV was confirmed by seroconversion in domestic birds and by the detection of specific neutralizing antibodies in 44% (11/25) of equids, 20.9% (14/67) of domestic birds, 4.7% (13/278) of free-ranging wild birds, 2.6% (2/78) of humans, and 1.5% (1/65) of small ruminants. Results indicate that not only equines but also humans and different species of domestic animals and wild birds were locally exposed to WNV. The detection of neutralizing antibodies for WNV in free-ranging individuals of abundant passerine species suggests that birds commonly found in the region may have been involved as amplifying hosts in local transmission cycles of WNV.
Reduced Relative Sensitivity of the Elecsys SARS-CoV-2 Antigen Assay in Saliva Compared to Nasopharyngeal Swabs
Authors: Audigé Annette , Böni Jürg , Schreiber Peter W. , Scheier Thomas , Buonomano Roberto , Rudiger Alain , Braun Dominique L. , Eich Gerhard , Keller Dagmar I. , Hasse Barbara , Berger Christoph , Günthard Huldrych F. , Manrique Amapola , Trkola Alexandra , Huber Michael
Published: August 2021

Early identification and isolation of SARS-CoV-2-infected individuals is central to contain the COVID-19 pandemic. Nasopharyngeal swabs (NPS) serve as a specimen for detection by RT-PCR and rapid antigen screening tests. Saliva has been confirmed as a reliable alternative specimen for RT-PCR and has been shown to be valuable for diagnosing children and in repetitive mass testing due to its non-invasive collection. Combining the advantages of saliva with those of antigen tests would be highly attractive to further increase test capacities. Here, we evaluated the performance of the Elecsys SARS-CoV-2 Antigen assay (Roche) in RT-PCR-positive paired NPS and saliva samples (N = 87) and unpaired NPS (N = 100) with confirmed SARS-CoV-2 infection (Roche cobas SARS-CoV-2 IVD test). We observed a high positive percent agreement (PPA) of the antigen assay with RT-PCR in NPS, reaching 87.2% across the entire cohort, whereas the overall PPA for saliva was insufficient (40.2%). At Ct values ≤ 28, PPA were 100% and 91.2% for NPS and saliva, respectively. At lower viral loads, the sensitivity loss of the antigen assay in saliva was striking. At Ct values ≤ 35, the PPA for NPS remained satisfactory (91.5%), whereas the PPA for saliva dropped to 46.6%. In conclusion, saliva cannot be recommended as a reliable alternative to NPS for testing with the Elecsys Anti-SARS-CoV-2 Antigen assay. As saliva is successfully used broadly in combination with RT-PCR testing, it is critical to create awareness that suitability for RT-PCR cannot be translated to implementation in antigen assays without thorough evaluation of each individual test system.
The Long-Term Effect of Industrial Waste Landfill on Surface Water. An Example from Central Poland
Authors: Wieczorek Kinga , Turek Anna , Kubicki Jakub , Wolf Wojciech M.
Published: August 2021

Water resources are influenced by many anthropogenic pollutants, which may migrate into the environment. The Bzura River is one of the rivers strongly impacted by human activity e.g., waste landfills and former industrial plants. The objective of this study was to assess the spatial-temporal variability of trace metals in river water and sediments and to recognize the main sources of pollution. An investigation was carried out on the 120 km section, during two hydrological years. Concentrations of Ni, Pb, Cd, Cu, Zn, Fe, and Mn were determined by atomic absorption spectrometry (AAS). The basic statistics were supported by principal component analysis (PCA) and cluster analysis (CA). Generally, only part of the results exceeded the WHO, USEPA, and Polish threshold values. However, one hotspot burdened by nickel was found. The spatial trend of Ni indicates long-distance migration. The PCA and CA confirmed that the Ni mainly comes from the old industrial waste landfill. Variability of the remaining metals was affected by more mixed sources. None of the metals showed any consistent temporal pattern. The PCA revealed that the anthropogenic factor explained over 50% of the variability in both years; therefore, intensive monitoring of the water river quality is still required.
Natural α-Glucosidase and Protein Tyrosine Phosphatase 1B Inhibitors: A Source of Scaffold Molecules for Synthesis of New Multitarget Antidiabetic Drugs
Authors: Genovese Massimo , Nesi Ilaria , Caselli Anna , Paoli Paolo
Published: August 2021

Diabetes mellitus (DM) represents a group of metabolic disorders that leads to acute and long-term serious complications and is considered a worldwide sanitary emergence. Type 2 diabetes (T2D) represents about 90% of all cases of diabetes, and even if several drugs are actually available for its treatment, in the long term, they show limited effectiveness. Most traditional drugs are designed to act on a specific biological target, but the complexity of the current pathologies has demonstrated that molecules hitting more than one target may be safer and more effective. The purpose of this review is to shed light on the natural compounds known as α-glucosidase and Protein Tyrosine Phosphatase 1B (PTP1B) dual-inhibitors that could be used as lead compounds to generate new multitarget antidiabetic drugs for treatment of T2D.
Production of Minor Ginsenosides C-K and C-Y from Naturally Occurring Major Ginsenosides Using Crude β-Glucosidase Preparation from Submerged Culture of Fomitella fraxinea
Authors: Kim Dae-Woon , Lee Won-Jae , Gebru Yoseph Asmelash , Upadhyaya Jitendra , Ko Sung-Ryong , Kim Young-Hoi , Kim Myung-Kon
Published: August 2021

Minor ginsenosides, such as compounds (C)-K and C-Y, possess relatively better bioactivity than those of naturally occurring major ginsenosides. Therefore, this study focused on the biotransformation of major ginsenosides into minor ginsenosides using crude β-glucosidase preparation isolated from submerged liquid culture of Fomitella fraxinea (FFEP). FFEP was prepared by ammonium sulfate (30–80%) precipitation from submerged culture of F. fraxinea. FFEP was used to prepare minor ginsenosides from protopanaxadiol (PPD)-type ginsenoside (PPDG-F) or total ginsenoside fraction (TG-F). In addition, biotransformation of major ginsenosides into minor ginsenosides as affected by reaction time and pH were investigated by TLC and HPLC analyses, and the metabolites were also identified by UPLC/negative-ESI-Q-TOF-MS analysis. FFEP biotransformed ginsenosides Rb1 and Rc into C-K via the following pathways: Rd → F2 → C-K for Rb1 and both Rd → F2→ C-K and C-Mc1 → C-Mc → C-K for Rc, respectively, while C-Y is formed from Rb2 via C-O. FFEP can be applied to produce minor ginsenosides C-K and C-Y from PPDG-F or TG-F. To the best of our knowledge, this study is the first to report the production of C-K and C-Y from major ginsenosides by basidiomycete F. fraxinea.
Biological Actions and Molecular Mechanisms of Sambucus nigra L. in Neurodegeneration: A Cell Culture Approach
Authors: Palomino Olga , García-Aguilar Ana , González Adrián , Guillén Carlos , Benito Manuel , Goya Luis
Published: August 2021

Sambucus nigra flowers (elderflower) have been widely used in traditional medicine for the relief of early symptoms of common cold. Its chemical composition mainly consists of polyphenolic compounds such as flavonoids, hydroxycinnamic acids, and triterpenes. Although the antioxidant properties of polyphenols are well known, the aim of this study is to assess the antioxidant and protective potentials of Sambucus nigra flowers in the human neuroblastoma (SH-SY5Y) cell line using different in vitro approaches. The antioxidant capacity is first evaluated by the oxygen radical absorbance capacity (ORAC) and the free radical scavenging activity (DPPH) methods. Cell viability is assessed by the crystal violet method; furthermore, the intracellular ROS formation (DCFH-DA method) is determined, together with the effect on the cell antioxidant defenses: reduced glutathione (GSH) and antioxidant enzyme activities (GPx, GR). On the other hand, mTORC1 hyperactivation and autophagy blockage have been associated with an increase in the formation of protein aggregates, this promoting the transference and expansion of neurodegenerative diseases. Then, the ability of Sambucus nigra flowers in the regulation of mTORC1 signaling activity and the reduction in oxidative stress through the activation of autophagy/mitophagy flux is also examined. In this regard, search for different molecules with a potential inhibitory effect on mTORC1 activation could have multiple positive effects either in the molecular pathogenic events and/or in the progression of several diseases including neurodegenerative ones.
Ionic Liquids for Development of Heterogeneous Catalysts Based on Nanomaterials for Biocatalysis
Authors: Wolny Anna , Chrobok Anna
Published: August 2021

The development of effective methods of enzyme stabilization is key for the evolution of biocatalytic processes. An interesting approach combines the stabilization process of proteins in ionic liquids and the immobilization of the active phase on the solid support. As a result, stable, active and heterogeneous biocatalysts are obtained. There are several benefits associated with heterogeneous processes, as easy separation of the biocatalyst from the reaction mixture and the possibility of recycling. Accordingly, this work focused on the supported ionic liquid phases as the efficient enzyme stabilization carriers, and their application in both continuous flow and batch biocatalytic processes.
Drug Targeting of Inflammatory Bowel Diseases by Biomolecules
Authors: Antunes Joana Costa , Seabra Catarina Leal , Domingues Joana Margarida , Teixeira Marta Oliveira , Nunes Cláudia , Costa-Lima Sofia Antunes , Homem Natália Cândido , Reis Salette , Amorim Maria Teresa Pessoa , Felgueiras Helena Prado
Published: August 2021

Inflammatory bowel disease (IBD) is a group of disabling, destructive and incurable immune-mediated inflammatory diseases comprising Crohn’s disease (CD) and ulcerative colitis (UC), disorders that are highly prevalent worldwide and demand a large investment in healthcare. A persistent inflammatory state enables the dysfunction and destruction of healthy tissue, hindering the initiation and endurance of wound healing. Current treatments are ineffective at counteracting disease progression. Further, increased risk of serious side effects, other comorbidities and/or opportunistic infections highlight the need for effective treatment options. Gut microbiota, the key to preserving a healthy state, may, alternatively, increase a patient’s susceptibility to IBD onset and development given a relevant bacterial dysbiosis. Hence, the main goal of this review is to showcase the main conventional and emerging therapies for IBD, including microbiota-inspired untargeted and targeted approaches (such as phage therapy) to infection control. Special recognition is given to existing targeted strategies with biologics (via monoclonal antibodies, small molecules and nucleic acids) and stimuli-responsive (pH-, enzyme- and reactive oxygen species-triggered release), polymer-based nanomedicine that is specifically directed towards the regulation of inflammation overload (with some nanosystems additionally functionalized with carbohydrates or peptides directed towards M1-macrophages). The overall goal is to restore gut balance and decrease IBD’s societal impact.
Risks of Obesity in Adolescence: The Role of Physical Activity in Executive Functions
Authors: Gameiro Fátima , Rosa Beatriz
Published: August 2021

The aim of this study was to analyze the efficiency of a physical activity program on executive performance in obese adolescents. Fifteen adolescents (5 males and 10 females), with a mean age of 14.73 years and an IMC mean of 36.74 participated in the study. None of the participants presented a compulsive eating disorder when screened by the Binge Eating Scale. A pretest and posttest assessment, twelve months later, was conducted by using a neuropsychological battery that evaluated the cognitive flexibility (Comprehensive Trail Making Test), inhibition control (Stroop Neuropsychological Screening Test and by the Frontal Assessment Battery), and planning (Tower of London). ANOVA of repeated measures was performed. The within-subjects tests demonstrated significant statistical differences between the two moments of evaluation at the level of inhibitory control and cognitive flexibility tasks, with higher performances in the second evaluation. These results suggest that the inclusion of obese adolescents in such programs may promote their executive capacities.
Development of Garments with Elastic Straps and Pressure Applicator (GESPA) and “GVcorrect” App to Follow the Changes in Lower-Extremity Alignment (Genu Valgum)—A Pilot Study
Authors: Tamm Anna-Liisa , Vaher Ivi , Linkberg Reet , Tilk Teet , Kritt Jana , Alviste Age , Sild Maarit , Vain Arved
Published: August 2021

Background: There are non-invasive methods of correcting genu valgum (GV), but to date, there is no method to evaluate mechanotherapeutic intervention that does not restrict child’s natural movements while the process is on-going so that timely decisions could be made on effectiveness of intervention. The aim of study was to develop and assess the comfortability of garments with elastic straps and pressure applicator (GESPA) and the reliability and user-friendliness of “GVcorrect” app, which aims to catch the elastic straps’ pressure level (mN). Methods: 6 children (5–7 y) with intermalleolar distance ≥5 cm wore GESPA daily for 3 months. Anthropometrical and goniometrical measurements were done according to standard technique; tone and biomechanical parameters of skeletal muscles determined with MyotonPRO; feedback about GESPA and “GVcorrect” collected via questionnaire. Results: Based on feedback from children and parents, new, more comfortable and user-friendly GESPA were designed; several updates were made to “GVcorrect” app; new goals were set for the next phase of the study. Conclusions: GESPA and the “GVcorrect” app serve their purpose, but there are still a number of important limitations that need to be removed before the product can be marketed. The study continues with product development until a medical device certificate is obtained.
Three-Dimensional Printing for Cancer Applications: Research Landscape and Technologies
Authors: Li Ruixiu , Ting Yu-Huan , Youssef Souha , Song Yunmei , Garg Sanjay
Published: August 2021

As a variety of novel technologies, 3D printing has been considerably applied in the field of health care, including cancer treatment. With its fast prototyping nature, 3D printing could transform basic oncology discoveries to clinical use quickly, speed up and even revolutionise the whole drug discovery and development process. This literature review provides insight into the up-to-date applications of 3D printing on cancer research and treatment, from fundamental research and drug discovery to drug development and clinical applications. These include 3D printing of anticancer pharmaceutics, 3D-bioprinted cancer cell models and customised nonbiological medical devices. Finally, the challenges of 3D printing for cancer applications are elaborated, and the future of 3D-printed medical applications is envisioned.
Revisiting the Cytotoxicity of Cationic Polyelectrolytes as a Principal Component in Layer-by-Layer Assembly Fabrication
Authors: Naumenko Ekaterina , Akhatova Farida , Rozhina Elvira , Fakhrullin Rawil
Published: August 2021

Polycations are an essential part of layer-by-layer (LbL)-assembled drug delivery systems, especially for gene delivery. In addition, they are used for other related applications, such as cell surface engineering. As a result, an assessment of the cytotoxicity of polycations and elucidation of the mechanisms of polycation toxicity is of paramount importance. In this study, we examined in detail the effects of a variety of water-soluble, positively charged synthetic polyelectrolytes on in vitro cytotoxicity, cell and nucleus morphology, and monolayer expansion changes. We have ranked the most popular cationic polyelectrolytes from the safest to the most toxic in relation to cell cultures. 3D cellular cluster formation was disturbed by addition of polyelectrolytes in most cases in a dose-dependent manner. Atomic force microscopy allowed us to visualize in detail the structures of the polyelectrolyte–DNA complexes formed due to electrostatic interactions. Our results indicate a relationship between the structure of the polyelectrolytes and their toxicity, which is necessary for optimization of drug and gene delivery systems.
The Cultivation of Industrial Hemp as Alternative Crop in a Less-Favoured Agricultural Area in Southern Italy: The Pignola Case Study
Authors: Satriani Antonio , Loperte Antonio , Pascucci Simone
Published: August 2021

Industrial hemp cultivation has the potential to be an environmentally friendly and highly sustainable crop and it can fit well in crop rotation practices to increase soil fertility. For this study, two commercial varieties of industrial hemp with low tetrahydrocannabinol (THC) content were used to test its reintroduction chance in the study area and to evaluate its response in terms of yield with respect to the soil and drought conditions of the area. During the vegetative period, non-invasive measures of the soil’s water status were performed using Watermark probes and ground penetrating radar. In addition, hemp crops vigour monitoring was performed by means of Sentinel 2 multispectral optical remote sensing data. In the absence of precipitation and/or irrigation, and with high consumption of soil water by the hemp crop due to evapotranspiration, the crop has completed its cycle thanks to its resistance to water stress conditions. From the soil water and satellite monitoring results, there is a good agreement with the field results in terms of water stress and its effects on crop vigour. This study contributes to a better understanding of the possibility of hemp crop reintroduction in areas where water deficit occurs, which could open up the opportunity for selecting hemp crop cultivars that can be grown under different agro-ecological conditions and are also of great commercial interest for decision makers involved in sustainable crop management and in the reduction of fertilizers and pollutants released into the environment.
Synthesis of a Reactive Template-Induced Core–Shell PZS@ZIF-67 Composite Microspheres and Its Application in Epoxy Composites
Authors: Song Kunpeng , Wang Yinjie , Ruan Fang , Yang Weiwei , Fang Zhuqing , Zheng Dongsen , Li Xueli , Li Nianhua , Qiao Meizhuang , Liu Jiping
Published: August 2021

Developing superior properties of epoxy resin composites with high fire resistance, light smoke, and low toxicity has been the focus of the research in the flame-retardant field. In particular, it is essential to decrease the emissions of toxic gases and smoke particles generated during the thermal decomposition of epoxy resin (EP) to satisfy the industrial requirements for environmental protection and safety. Consequently, the PZS@ZIF-67 composite was designed and synthesized by employing the hydroxyl group-containing polyphosphazene (poly(cyclotriphosphazene-co-4,4′-dihydroxydiphenylsulfone), PZS) as both the interfacial compatibility and an in situ template and the ZIF-67 nanocrystal as a nanoscale coating and flame-retardant cooperative. ZIF-67 nanocrystal with multidimensional nanostructures was uniformly wrapped on the surface of PZS microspheres. Subsequently, the acquired PZS@ZIF-67 composite was incorporated into the epoxy resin to prepare composite samples for the study of their fire safety, toxicity suppression, and mechanical performance. Herein, the EP/5% PZS@ZIF-67 passed the V-0 rating in a UL-94 test with a 31.9% limit oxygen index value. More precisely, it is endowed with a decline of 51.08%, 28.26%, and 37.87% of the peak heat release rate, the total heat release, and the total smoke production, respectively. In addition, the unique structure of PZS@ZIF-67 microsphere presented a slight impact on the mechanical properties of EP composites at low loading. The PZS@ZIF-67 possible flame-retardant mechanism was speculated based on the analysis of the condensed phase and the gas phase of EP composites.
Study of Mechanical Properties of PHBHV/Miscanthus Green Composites Using Combined Experimental and Micromechanical Approaches
Authors: Lemaire Thibault , Rodi Erica Gea , Langlois Valérie , Renard Estelle , Sansalone Vittorio
Published: August 2021

In recent years the interest in the realization of green wood plastic composites (GWPC) materials has increased due to the necessity of reducing the proliferation of synthetic plastics. In this work, we study a specific class of GWPCs from its synthesis to the characterization of its mechanical properties. These properties are related to the underlying microstructure using both experimental and modeling approaches. Different contents of Miscanthus giganteus fibers, at 5, 10, 20, 30 weight percent’s, were thus combined to a microbial matrix, namely poly (3-hydroxybutyrate)-co-poly(3-hydroxyvalerate) (PHBHV). The samples were manufactured by extrusion and injection molding processing. The obtained samples were then characterized by cyclic-tensile tests, pycnometer testing, differential scanning calorimetry, Fourier transform infrared spectroscopy, X-ray diffraction, and microscopy. The possible effect of the fabrication process on the fibers size is also checked. In parallel, the measured properties of the biocomposite were also estimated using a Mori–Tanaka approach to derive the effective behavior of the composite. As expected, the addition of reinforcement to the polymer matrix results in composites with higher Young moduli on the one hand, and lower failure strains and tensile strengths on the other hand (tensile modulus was increased by 100% and tensile strength decreased by 23% when reinforced with 30 wt % of Miscanthus fibers).
Removal of a Past Varnish Treatment from a 19th-Century Belgian Wall Painting by Means of a Solvent-Loaded Double Network Hydrogel
Authors: Al-Emam Ehab , Beltran Victoria , De Meyer Steven , Nuyts Gert , Wetemans Vera , De Wael Karolien , Caen Joost , Janssens Koen
Published: August 2021

Polymeric materials have been used by painting conservator-restorers as consolidants and/or varnishes for wall paintings. The application of these materials is carried out when confronting loose paint layers or as a protective coating. However, these materials deteriorate and cause physiochemical alterations to the treated surface. In the past, the monumental neo-gothic wall painting ‘The Last Judgment’ in the chapel of Sint-Jan Berchmanscollege in Antwerp, Belgium was treated with a synthetic polymeric material. This varnish deteriorated significantly and turned brown, obscuring the paint layers. Given also that the varnish was applied to some parts of the wall painting and did not cover the entire surface, it was necessary to remove it in order to restore the original appearance of the wall painting. Previous attempts carried out by conservator-restorers made use of traditional cleaning methods, which led to damage of the fragile paint layers. Therefore, gel cleaning was proposed as a less invasive and more controllable method for gently softening and removing the varnish. The work started by identifying the paint stratigraphy and the deteriorated varnish via optical microscopy (OM), scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDX), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy. A polyvinyl alcohol–borax/agarose (PVA–B/AG) hydrogel loaded with a number of solvents/solvent mixtures was employed in a series of tests to select the most suitable hydrogel composite. By means of the hydrogel composite loaded with 10% propylene carbonate, it was possible to safely remove the brown varnish layer. The results were verified by visual examinations (under visible light ‘VIS’ and ultraviolet light ‘UV’) as well as OM and FTIR spectroscopy.
A Simulation-Data-Based Machine Learning Model for Predicting Basic Parameter Settings of the Plasticizing Process in Injection Molding
Authors: Schmid Matthias , Altmann Dominik , Steinbichler Georg
Published: August 2021

The optimal machine settings in polymer processing are usually the result of time-consuming and expensive trials. We present a workflow that allows the basic machine settings for the plasticizing process in injection molding to be determined with the help of a simulation-driven machine learning model. Given the material, screw geometry, shot weight, and desired plasticizing time, the model is able to predict the back pressure and screw rotational speed required to achieve good melt quality. We show how data sets can be pre-processed in order to obtain a generalized model that performs well. Various supervised machine learning algorithms were compared, and the best approach was evaluated in experiments on a real machine using the predicted basic machine settings and three different materials. The neural network model that we trained generalized well with an overall absolute mean error of 0.27% and a standard deviation of 0.37% on unseen data (the test set). The experiments showed that the mean absolute errors between the real and desired plasticizing times were sufficiently small, and all predicted operating points achieved good melt quality. Our approach can provide the operators of injection molding machines with predictions of suitable initial operating points and, thus, reduce costs in the planning phase. Further, this approach gives insights into the factors that influence melt quality and can, therefore, increase our understanding of complex plasticizing processes.
The Toxicity of Universal Dental Adhesives: An In Vitro Study
Authors: Wawrzynkiewicz Adam , Rozpedek-Kaminska Wioletta , Galita Grzegorz , Lukomska-Szymanska Monika , Lapinska Barbara , Sokolowski Jerzy , Majsterek Ireneusz
Published: August 2021

There is no consensus in the literature regarding the potential toxicity of universal dental adhesives (UDA). Being used in close proximity to the pulp, their biocompatibility should be an important factor in dental research. The aim of the present study was to evaluate the biocompatibility of UDA in an in vitro model. The study was performed using a monocyte/macrophage peripheral blood SC cell line (ATCC CRL-9855) on four specific UDA, namely: All-Bond Universal (Bisco); CLEARFIL Universal Bond Quick (Kuraray); G-Premio BOND (GC); Single Bond Universal (3M ESPE). The cytotoxicity of the investigated UDA was measured using the XTT colorimetric assay. The genotoxicity of the analyzed compounds was evaluated using an alkaline version of the comet assay. Furthermore, flow cytometry (FC) apoptosis detection was performed using the FITC Annexin V Apoptosis Detection Kit I. FC cell-cycle arrest assessment was performed using propidium iodide staining. The study observed significant differences in the toxicity of the UDA that were tested, as G-Premio BOND showed significant in vitro toxicity in all of the tests performed, while All-Bond Universal, CLEARFIL Universal Bond Quick and Single Bond Universal did not present any significant toxic effects toward SC cell line. The in vitro toxicity of UDA should be taken into consideration prior to in vivo and clinical studies. The flow cytometry could improve the accuracy of dental materials research and should be incorporated into the standardization criteria.
Protective Multifunctional Fibrous Systems Based on Natural Fibers and Metal Oxide Nanoparticles
Authors: Araújo Joana C. , Fangueiro Raul , Ferreira Diana P.
Published: August 2021

In recent years, an unprecedented increase in the development of products and technologies to protect the human being has been observed. Now, more than ever, the world population is exposed to several threats, harmful to their well-being and health. Chemical and biological hazardous agents stand out as one of the biggest threats, not only for the military forces, but also for the civilians. Consequently, it’s essential to develop personal protective systems that are able to protect their user, not only passively, but actively, being able to detect, adsorb, degrade and decontaminate pesticides, pollutants, microorganisms and most importantly: chemical/biological warfare agents. One recent strategy for the development of active fibrous structures with improved functions and new properties is their functionalization with nanoparticles (NPs), especially metal oxides. Although their known effectiveness in the decomposition of harmful agents, the NPs could also include other functionalities in the same structure using low quantities of material, without adding extra weight, which is of huge importance for a soldier in the battlefield. The use of natural fibers as the substrate is also very interesting, since this material is a much sustainable alternative when compared to synthetic ones, also providing excellent properties.
A Review on Molecularly Imprinted Polymers Preparation by Computational Simulation-Aided Methods
Authors: Liu Zhimin , Xu Zhigang , Wang Dan , Yang Yuming , Duan Yunli , Ma Liping , Lin Tao , Liu Hongcheng
Published: August 2021

Molecularly imprinted polymers (MIPs) are obtained by initiating the polymerization of functional monomers surrounding a template molecule in the presence of crosslinkers and porogens. The best adsorption performance can be achieved by optimizing the polymerization conditions, but this process is time consuming and labor-intensive. Theoretical calculation based on calculation simulations and intermolecular forces is an effective method to solve this problem because it is convenient, versatile, environmentally friendly, and inexpensive. In this article, computational simulation modeling methods are introduced, and the theoretical optimization methods of various molecular simulation calculation software for preparing molecularly imprinted polymers are proposed. The progress in research on and application of molecularly imprinted polymers prepared by computational simulations and computational software in the past two decades are reviewed. Computer molecular simulation methods, including molecular mechanics, molecular dynamics and quantum mechanics, are universally applicable for the MIP-based materials. Furthermore, the new role of computational simulation in the future development of molecular imprinting technology is explored.
An Insight into Nano Silver Fluoride-Coated Silk Fibroin Bioinspired Membrane Properties for Guided Tissue Regeneration
Authors: Pandey Aditi , Yang Tzu-Sen , Yang Ta-I , Belem Wendimi Fatimata , Teng Nai-Chia , Chen I-Wen , Huang Ching-Shuan , Kareiva Aivaras , Yang Jen-Chang
Published: August 2021

The current work focuses on the development of a novel electrospun silk fibroin (SF) nonwoven mat as a GTR membrane with antibacterial, biomineralization and biocompatible properties. The γ-poly glutamic acid (γ-PGA)-capped nano silver fluoride (NSF) and silver diamine fluoride (SDF) were first synthesized, which were dip-coated onto electrospun silk fibroin mats (NSF-SF and SDF-SF). UV-Vis spectroscopy and TEM depicted the formation of silver nanoparticles. NSF-SF and SDF-SF demonstrated antibacterial properties (against Porphyromonas gingivalis) with 3.1 and 6.7 folds higher relative to SF, respectively. Post-mineralization in simulated body fluid, the NSF-SF effectively promoted apatite precipitation (Ca/P ~1.67), while the SDF-SF depicted deposition of silver nanoparticles, assessed by SEM-EDS. According to the FTIR-ATR deconvolution analysis, NSF-SF portrayed ~75% estimated hydroxyapatite crystallinity index (CI), whereas pure SF and SDF-SF demonstrated ~60%. The biocompatibility of NSF-SF was ~82% when compared to the control, while SDF-coated samples revealed in vitro cytotoxicity, further needing in vivo studies for a definite conclusion. Furthermore, the NSF-SF revealed the highest tensile strength of 0.32 N/mm and 1.76% elongation at break. Therefore, it is substantiated that the novel bioactive and antibacterial NSF-SF membranes can serve as a potential candidate, shedding light on further in-depth analysis for GTR applications.
The Effect of Fibrous Reinforcement on the Polycondensation Degree of Slag-Based Alkali Activated Composites
Authors: Lancellotti Isabella , Piccolo Federica , Nguyen Hoang , Mastali Mohammad , Alzeer Mohammad , Illikainen Mirja , Leonelli Cristina
Published: August 2021

Alternative cementitious binders, based on industrial side streams, characterized by a low carbon footprint, are profitably proposed to partially replace Portland cement. Among these alternatives, alkali-activated materials have attracted attention as a promising cementitious binder. In this paper, the chemical stability of the matrix, in fiber-reinforced slag-based alkali-activated composites, was studied, in order to assess any possible effect of the presence of the reinforcement on the chemistry of polycondensation. For this purpose, organic fiber, cellulose, and an inorganic fiber, basalt, were chosen, showing a different behavior in the alkaline media that was used to activate the slag fine powders. The novelty of the paper is the study of consolidation by means of chemical measurements, more than from the mechanical point of view. The evaluation of the chemical behavior of the starting slag in NaOH, indeed, was preparatory to the understanding of the consolidation degree in the alkali-activated composites. The reactivity of alkali-activated composites was studied in water (integrity test, normed leaching test, pH and ionic conductivity), and acids (leaching in acetic acid and HCl attack). The presence of fibers does not favor nor hinder the geopolymerization process, even if an increase in the ionic conductivity in samples containing fibers leads to the hypothesis that samples with fibers are less consolidated, or that fiber dissolution contributes to the conductivity values. The amorphous fraction was enriched in silicon after HCl attack, but the structure was not completely dissolved, and the presence of an amorphous phase is confirmed (C–S–H gel). Basalt fibers partly dissolved in the alkaline environment, leading to the formation of a C–N–A–S–H gel surrounding the fibers. In contrast, cellulose fiber remained stable in both acidic and alkaline conditions.