bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Urban Ventilation Corridor System Using Meteorology and GIS Technology: A Case Study in Zhengzhou, China
Authors: Pan Pan , Li Fengxiu , Zhu Yeyu , Xu Pengpeng , Shang Yulong , Liao Rongwei
Published: August 2024

Urban ventilation corridors are designed to enhance air quality, alleviate urban thermal conditions, reduce pollution and energy consumption, as well as improve human comfort within cities. They play a pivotal role in mitigating environmental impacts, particularly in densely populated urban areas. Based on satellite remote sensing data, meteorological observations, basic geographic information of Zhengzhou City and its surroundings, and urban planning data, we analyzed the urban wind environment, urban heat island, ecological cold sources, and ventilation potential. The findings reveal several key insights: (1) Dominant winds in Zhengzhou City predominantly originate from the northwest, northeast, and south, influenced by topography and the monsoon climate, with seasonal variations. These wind patterns are crucial considerations for designing primary ventilation corridors. (2) The urban heat island exhibits a polycentric spatial distribution, with intensity decreasing from the city center towards the periphery. Ecological cold sources, primarily situated in the city outskirts, act as reservoirs of fresh air that mitigate the urban heat island effect through designated corridors. (3) A preliminary corridor system, termed “eight primary and thirteen secondary corridors”, is proposed for Zhengzhou City based on an integrated assessment of ventilation potential, urban surface roughness, and sky view factor. This research contributes to advancing the understanding of urban ventilation systems and provides practical insights for policymakers, urban planners, and researchers seeking sustainable solutions to mitigate climate impacts in rapidly urbanizing environments in the region.
On Fourier Series in the Context of Jacobi Matrices
Authors: Matos José M. A. , Vasconcelos Paulo B. , Matos José A. O.
Published: August 2024

We investigate the properties of matrices that emerge from the application of Fourier series to Jacobi matrices. Specifically, we focus on functions defined by the coefficients of a Fourier series expressed in orthogonal polynomials. In the operational formulation of integro-differential problems, these infinite matrices play a fundamental role. We have derived precise calculation formulas for their elements, enabling exact computation of these operational matrices. Numerical results illustrate the effectiveness of our approach.
A Y-Type Air-Cooled Battery Thermal Management System with a Short Airflow Path for Temperature Uniformity
Authors: Li Xiangyang , Liu Jing , Li Xiaomin
Published: August 2024

A Y-type air-cooled structure has been proposed to improve the heat dissipation efficiency and temperature uniformity of battery thermal management systems (BTMSs) by reducing the flow path of air. By combining computational fluid dynamics (CFD) methods, the influence of the depths of the distribution and convergence plenums on the airflow velocity through battery cells was analyzed to improve heat dissipation efficiency. Adjusting the width of the first and ninth cooling channels can change the air velocity of these two channels, thereby improving the temperature uniformity of the BTMS. Further discussion was conducted regarding the influences of inlet and outlet depths. When the inlet width and outlet width were 20 mm, the maximum temperature and maximum temperature difference of the Y-type BTMS were 39.84 °C and 0.066 °C at a discharge rate of 2.5 °C, respectively; these temperatures were 1.537 °C (3.68%) and 0.059 °C (47.2%) lower than those of the T-type model. Meanwhile, the energy consumption of the sample also decreased by 13.1%. The results indicate that the heat dissipation performance of the proposed Y-type BTMS was improved, achieving excellent temperature uniformity, and the energy consumption was also reduced.
Tillandsia usneoides Ethanolic Extract Induces Cytotoxicity in SW480 Colon Cancer Cell Line via PPARγ Modulation
Authors: López María Paula , Jiménez María Camila , Contreras Julián Esteban , Rojas Laura , Fiorentino Susana , Iglesias José
Published: August 2024

Colorectal cancer (CRC) is a prevalent and deadly tumor worldwide. Understanding the molecular mechanisms underlying CRC development will improve treatment outcomes and patient survival. Natural molecules and metabolites from plants, such as Tillandsia usneoides, reduce tumor growth by modulating glucose metabolism and increasing reactive oxygen species (ROS). To shed light on the mechanism involved in the anti-tumor effects of T. usneoides, we evaluated the cytotoxic effect of the ethanolic extract of this plant on the colon cancer cell line SW480 through the activation of the peroxisome proliferator-activated receptor gamma (PPARγ), a nuclear receptor that plays a role on lipid metabolism and inflammation in cancer cells. To this end, we assessed the activation of PPARγ by T. usneoides extract in transactivation luciferase assays, as well as the cytotoxic effect of this extract on the SW480 cell line after knocking down PPARγ using shRNA. Our findings indicate that the T. usneoides extract exhibits cytotoxic effects on the SW480 cell line, potentially in the same way as PPARγ activator, pioglitazone, i.e., by increasing reactive oxygen species (ROS). In addition, both T. usneoides extract and pioglitazone exert lipogenic properties in the SW480 cells. Taken together, these results demonstrate that the T. usneoides extract decreases the viability of the colon cancer cell line SW480, at least in part, through the activation of PPARγ. This suggests the potential for further use of this plant in the treatment of other chronic diseases.
Reproductive Strategies and Embryonic Development of Autumn-Spawning Bitterling (Acheilognathus rhombeus) within the Mussel Host
Authors: Kim Hyeongsu , Choe Jongryeol , Ko Myeonghun
Published: August 2024

We investigated the reproductive strategies and embryonic development of Acheilognathus rhombeus (a bitterling species that spawns in autumn) within its freshwater mussel host in the Bongseo Stream, South Korea. By focusing on survival mechanisms during critical stages of embryonic development, the selective use of mussel gill demibranchs by the bitterlings and associated adaptive traits were observed over 1 year. A significant diapause phase occurs at developmental stage D, which lasts for approximately 7 months, allowing embryos to survive winter. Development resumes when the temperature exceeds 10 °C. Minute tubercles on the embryos (crucial for anchoring within the host gill demibranchs and preventing premature ejection) exhibit the largest height during diapause, and the height decreases when developmental stage E is reached, when growth resumes. Acheilognathus rhombeus embryos were observed in 30.5% of the mussels, mostly within the inner gills, thereby maximizing spatial use and oxygen access to enhance survival. These results highlight the intricate relationship between A. rhombeus and its mussel hosts, demonstrating the evolutionary adaptations that enhance reproductive success and survival. This study provides valuable insights into the ecological dynamics and conservation requirements of such symbiotic relationships.
Bacterial Purine Nucleoside Phosphorylases from Mesophilic and Thermophilic Sources: Characterization of Their Interaction with Natural Nucleosides and Modified Arabinofuranoside Analogues
Authors: Bychek Irina A. , Zenchenko Anastasia A. , Kostromina Maria A. , Khisamov Marat M. , Solyev Pavel N. , Esipov Roman S. , Mikhailov Sergey N. , Varizhuk Irina V.
Published: August 2024

The enzymatic synthesis of nucleoside derivatives is an important alternative to multi-step chemical methods traditionally used for this purpose. Despite several undeniable advantages of the enzymatic approach, there are a number of factors limiting its application, such as the limited substrate specificity of enzymes, the need to work at fairly low concentrations, and the physicochemical properties of substrates—for example, low solubility. This research conducted by our group is dedicated to the advantages and limitations of using purine nucleoside phosphorylases (PNPs), the main enzymes for the metabolic reutilization of purines, in the synthesis of modified nucleoside analogues. In our work, the substrate specificity of PNP from various bacterial sources (mesophilic and thermophilic) was studied, and the effect of substrate, increased temperature, and the presence of organic solvents on the conversion rate was investigated.
Radiosensitizing Effect of PARP Inhibition on Chondrosarcoma and Chondrocyte Cells Is Dependent on Radiation LET
Authors: Gilbert Antoine , Tudor Mihaela , Delaunay Amandine , Leman Raphaël , Levilly Julien , Atkinson Alexandre , Castéra Laurent , Dinischiotu Anca , Savu Diana Iulia , Valable Samuel , Chevalier François
Published: August 2024

Chondrosarcoma is a rare malignant tumor that forms in bone and cartilage. The primary treatment involves surgical removal of the tumor with a margin of healthy tissue. Especially if complete surgical removal is not possible, radiation therapy and chemotherapy are used in conjunction with surgery, but with a generally low efficiency. Ongoing researches are focused on understanding the genetic and molecular basis of chondrosarcoma following high linear energy transfer (LET) irradiation, which may lead to treatments that are more effective. The goal of this study is to evaluate the differential effects of DNA damage repair inhibitors and high LET irradiation on chondrosarcoma versus chondrocyte cells and the LET-dependency of the effects. Two chondrosarcoma cell lines with different IDH mutation status and one chondrocyte cell line were exposed to low LET (X-ray) and high LET (carbon ion) irradiation in combination with an Olaparib PARP inhibitor. Cell survival and DNA repair mechanisms were investigated. High LET irradiation drastically reduced cell survival, with a biological efficiency three times that of low LET. Olaparib significantly inhibited PARylation in all the tested cells. A significant reduction in cell survival of both chondrosarcoma and chondrocyte cells was observed following the treatment combining Olaparib and X-ray. PARP inhibition induced an increase in PARP-1 expression and a reduced effect on the cell survival of WT IDH chondrosarcoma cells. No radiosensitizing effect was observed in cells exposed to Olaparib paired with high LET irradiation. NHEJ was activated in response to high LET irradiation, neutralizing the PARP inhibition effect in both chondrosarcoma cell lines. When high LET irradiation is not available, PARP inhibition could be used in combination with low LET irradiation, with significant radiosensitizing effects on chondrosarcoma cells. Chondrocytes may be affected by the treatment combination too, showing the need to preserve normal tissues from radiation fields when this kind of treatment is suggested.
Post-Herpetic Anti-NMDAR Encephalitis in Denmark: Current Status and Future Challenges
Authors: Søgaard Anna , Poulsen Charlotte Aaberg , Belhouche Nadia Zeeberg , Thybo Alberte , Hovet Siv Tonje Faret , Larsen Lykke , Nilsson Christine , Blaabjerg Morten , Nissen Mette Scheller
Published: August 2024

It is well known that N-methyl-D-aspartate receptor encephalitis (NMDARE) can be triggered by infectious encephalitis such as herpes simplex virus 1 encephalitis (HSE). However, the incidence of post-HSE NMDARE in Denmark is unknown. We reviewed literature cases and compared these to retrospectively identified cases of post-HSE NMDARE in Denmark, using a national cohort database of autoimmune encephalitis (AE) and two regional databases of infectious encephalitis patients. We identified 80 post-HSE NMDARE cases in the literature, 66% being children, who more often presented movement disorders, decreased consciousness, and sleep disturbances compared to adults. Eight patients with post-HSE NMDARE were identified from the national cohort database of AE, none being children. Forty-four HSE patients were identified from the regional infectious encephalitis databases. Of these, 16 (36%) fulfilled the Graus criteria for probable/definite NMDARE, and eight (18%) presented a prolonged/relapsing disease course. Ten (23%) were tested for AE during hospitalization. Six (14%) had leftover cerebrospinal fluid available for retrospective autoantibody testing. One out of these six patients (17%) harbored NMDARE antibodies. Thus, in total, nine post-HSE NMDARE patients have been identified in Denmark from 2009 to 2021. Comparing the adult Danish patients to the literature, Danish patients were older, but the clinical phenotype and paraclinical findings were similar. Overall, the incidence of adult post-HSE NMDARE in the Region of Southern Denmark was 0.17 per million people per year and only 7% of adult HSE patients in the region were diagnosed with post-HSE NMDARE. Our findings suggest that adult patients are still underdiagnosed and the absence of pediatric cases diagnosed with post-HSE NMDARE in Denmark is highly concerning.
Development and Validation of Machine Learning Algorithms for Prediction of Colorectal Polyps Based on Electronic Health Records
Authors: Ba Qinwen , Yuan Xu , Wang Yun , Shen Na , Xie Huaping , Lu Yanjun
Published: August 2024

Background: Colorectal Polyps are the main source of precancerous lesions in colorectal cancer. To increase the early diagnosis of tumors and improve their screening, we aimed to develop a simple and non-invasive diagnostic prediction model for colorectal polyps based on machine learning (ML) and using accessible health examination records. Methods: We conducted a single-center observational retrospective study in China. The derivation cohort, consisting of 5426 individuals who underwent colonoscopy screening from January 2021 to January 2024, was separated for training (cohort 1) and validation (cohort 2). The variables considered in this study included demographic data, vital signs, and laboratory results recorded by health examination records. With features selected by univariate analysis and Lasso regression analysis, nine machine learning methods were utilized to develop a colorectal polyp diagnostic model. Several evaluation indexes, including the area under the receiver-operating-characteristic curve (AUC), were used to compare the predictive performance. The SHapley additive explanation method (SHAP) was used to rank the feature importance and explain the final model. Results: 14 independent predictors were identified as the most valuable features to establish the models. The adaptive boosting machine (AdaBoost) model exhibited the best performance among the 9 ML models in cohort 1, with accuracy, sensitivity, specificity, positive predictive value, negative predictive value, F1 score, and AUC (95% CI) of 0.632 (0.618–0.646), 0.635 (0.550–0.721), 0.674 (0.591–0.758), 0.593 (0.576–0.611), 0.673 (0.654–0.691), 0.608 (0.560–0.655) and 0.687 (0.626–0.749), respectively. The final model gave an AUC of 0.675 in cohort 2. Additionally, the precision recall (PR) curve for the AdaBoost model reached the highest AUPR of 0.648, positioning it nearest to the upper right corner. SHAP analysis provided visualized explanations, reaffirming the critical factors associated with the risk of colorectal polyps in the asymptomatic population. Conclusions: This study integrated the clinical and laboratory indicators with machine learning techniques to establish the predictive model for colorectal polyps, providing non-invasive, cost-effective screening strategies for asymptomatic individuals and guiding decisions for further examination and treatment.
The Neuroprotective and Anxiolytic Effects of Magnesium Sulfate on Retinal Dopaminergic Neurons in 6-OHDA-Induced Parkinsonian Rats: A Pilot Study
Authors: Huang Leyi , Lin Renxi , Zhang Chunying , Zheng Shaoqing , Wang Yiyang , Wu Zeyu , Chen Sihao , Shen Yihan , Zhang Guoheng , Qi Yuanlin , Lin Ling
Published: August 2024

This study investigates the protective effects of magnesium sulfate on dopamine neurons in the retinas of rats with 6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD). Rapidly progressing cognitive decline often precedes or coincides with the motor symptoms associated with PD. PD patients also frequently exhibit visual function abnormalities. However, the specific mechanisms underlying visual dysfunction in PD patients are not yet fully understood. Therefore, this study aims to investigate whether magnesium homeostasis affects dopaminergic neurons in the retina of PD rats. Thirty-six rats were divided into four groups: (1) control, (2) control with magnesium sulfate (control/MgSO4), (3) Parkinson’s disease (PD), and (4) Parkinson’s disease with magnesium sulfate (PD/MgSO4). The apomorphine-induced (APO) rotation test assessed the success of the PD models. The open-field experiment measured the rats’ anxiety levels. Tyrosine hydroxylase (TH) and glutamate levels, indicators of dopamine neuron survival, were detected using immunofluorescence staining. Protein levels of solute carrier family 41 A1 (SCL41A1), magnesium transporter 1 (MagT1), and cyclin M2 (CNNM2) in the retina were analyzed using Western blot. Results showed that, compared to the PD group, rats in the PD/MgSO4 group had improved psychological states and motor performance at two and four weeks post-surgery. The PD/MgSO4 group also exhibited significantly higher TH fluorescence intensity in the left retinas and lower glutamate fluorescence intensity than the PD group. Additional experiments indicated that the protein levels of SLC41A1, MagT1, and CNNM2 were generally higher in the retinas of the PD/MgSO4 group, along with an increase in retinal magnesium ion content. This suggests that magnesium sulfate may reduce glutamate levels and protect dopamine neurons in the retina. Thus, magnesium sulfate might have therapeutic potential for visual functional impairments in PD patients.
Patients’ Perspectives on the Acceptability and Effectiveness of a Community Health Worker-Led Intervention to Increase Chronic Kidney Disease Knowledge and Screening among Underserved Latine Adults: The CARE 2.0 Study
Authors: Diaz-Martinez Janet , Delgado-Enciso Ivan , Duran Carlos , Kallus Laura , Jean-Pierre Aydeivis , Lopez Brenda , Mancilla Jessica , Madruga Yoel , Hernandez-Fuentes Gustavo A. , Kotzker Wayne , Delgado-Enciso Osiris , Wagner Eric , Hospital Michelle
Published: August 2024

In the United States, Chronic Kidney Disease (CKD) affects approximately 1 in 7 adults. Despite its significant impact, CKD awareness, education, and screening are often lacking among underserved Latine populations, leading to poorer health outcomes and higher mortality rates. Various studies highlight the crucial role of Community Health Workers (CHWs) in improving health outcomes within minority communities both domestically and globally. However, there remains a gap in research on the acceptance and effectiveness of CHW-led interventions targeting CKD. This prospective intervention study employed a pre-post quasi-experimental design to evaluate a CHW-led educational program aimed at enhancing CKD knowledge, screening, and monitoring among Latines with low health literacy and English proficiency. CHWs utilized a culturally tailored CKD Flipchart, and 100 underserved patients received the intervention. Feedback from 85 participants who completed post-intervention surveys indicated high satisfaction with the program’s relevance and the professionalism of the CHWs. Importantly, 85% expressed a positive intention to seek kidney care following the intervention. Preliminary analysis of medical records before and after the intervention showed improvements in glycemic control (median change = −18.0, p = 0.014) and triglyceride levels (median change = −29.0, p = 0.035), suggesting the program’s effectiveness in managing CKD risk factors. These findings highlight the potential of CHW-led interventions to reduce kidney health disparities among underserved communities.
RETRACTED: Yang et al. How Does Physical Activity Enhance the Subjective Well-Being of University Students? A Chain Mediation of Cognitive Reappraisal and Resilience. Behav. Sci. 2024, 14, 164
Authors: Yang Mengmeng , Wu Ji , Wu Yigang , Li Xinxing
Published: August 2024

The journal Behavioral Sciences retracts the article titled, “How Does Physical Activity Enhance the Subjective Well-Being of University Students [...]
Adult Attachment and Emotion Regulation Flexibility in Romantic Relationships
Authors: Mosannenzadeh Farnaz , Luijten Maartje , MacIejewski Dominique F. , Wiewel Grace V. , Karremans Johan C.
Published: August 2024

Adults with attachment insecurity often struggle in romantic relationships due to difficulties in emotion regulation (ER). One potentially influential yet understudied factor is the inflexible over-reliance on either intrapersonal (self-directed, e.g., suppression) or interpersonal (involving others, e.g., sharing) ER. This study investigates the association between attachment insecurity and flexibility in using interpersonal versus intrapersonal ER in response to daily stressors in romantic relationships. We hypothesized that higher attachment avoidance and anxiety are associated with (H1) higher reliance on either intrapersonal or interpersonal ER over the other, respectively; (H2) less variable use of interpersonal compared to intrapersonal ER over time; and (H3) less flexible use of interpersonal compared to intrapersonal ER depending on the availability of a romantic partner. Study 1 (N = 174; 133 females, Mage = 23.79, SDage = 7.63) used an online cross-sectional survey to measure average inter/intrapersonal ER, addressing H1. Study 2 (N = 124; 104 females, Mage = 22.45, SDage = 6.39), combined a baseline survey with experience sampling (7 days, 8 notifications/day), addressing H1, H2, and H3. Results showed that higher attachment avoidance was associated with lower interpersonal compared to intrapersonal ER. Higher attachment anxiety was associated with less variable use of interpersonal compared to intrapersonal ER and less flexible use of interpersonal ER depending on partner availability. These findings suggest distinct associations between attachment orientations and ER flexibility, explaining ER difficulties in individuals with high attachment insecurity.
Geospatial Analysis of the Distribution of Energy Poverty in the Residential Sector in the Valencian Community
Authors: Sujar-Cost Adrián , Lorenzo-Sáez Edgar , Arce Victoria Lerma , Aliaga Eloina Coll
Published: August 2024

This study addresses energy poverty, a situation where households are unable to afford the minimum energy services required to meet their basic needs, a problem with profound implications for the well-being of vulnerable populations. To identify the most affected areas, a geospatial analysis of energy poverty distribution in the Valencian Community (Spain) was conducted using a correlation study between the global vulnerability index and the energy poverty index, calculated from residential energy certificates. The results highlight areas of significant social, economic, demographic, and residential vulnerability, as well as regions with very high or very low energy consumption. Specifically, regions with high energy poverty and vulnerability indices were identified, enabling targeted interventions. The study concludes that interventions in these identified areas are essential to mitigate the adverse effects of energy poverty and improve living conditions for affected populations. This research offers a novel methodology for mapping regional energy poverty, surpassing previous studies in precision for identifying and addressing vulnerable areas.
Dynamic Performance Analysis of Precast Segment Column Reinforced with CFRP Subject to Vehicle Collision
Authors: Han Runbo , Zhou Hai , Wu Min
Published: August 2024

With the extensive use of a precast segment column in the field of engineering, impact resistance performance has gradually attracted attention, as many accidents have caused huge economic losses and casualties. This study explored the dynamic response and failure modes of a prefabricated segment column both with and without Carbon Fiber-Reinforced Polymers (CFRPs). Firstly, numerical models of a precast segment column and CFRP-wrapped steel column under impact loads were developed, and the modeling method’s accuracy was fully verified. Then, numerical models of a bridge precast segment column with and without CFRPs under vehicle collision were established, and the differences in the dynamic performances between the precast segment column with and without CFRPs are explored. Finally, the effects of impact velocity, concrete strength, and CFRP thickness on the dynamic performance of a precast segment column are considered. The results indicate that in the case of a vehicle collision, multiple cross-sectional positions form highly complex stress states. At 100 km/h, the differences in bending moment and shear force values between reinforced and unreinforced precast segment columns at the impact section are 7.6% and 7.1%, respectively. At this velocity, the peak impact force also increases by 15.8% as the local stiffness of the precast segment column increases after reinforcement with a CFRP. The bottom segment of the precast segment column with a CFRP is crushed, and the precast segment column experiences shear failure under vehicle collision.
Measuring Vibrations of Subway Tunnel Structures with Cracks
Authors: Zhou Qihui , Zhang Qiongfang , Sun Miaomiao , Huang Xin , Huang Zhanggong , Wen Xuewei , Qiu Yuanfeng
Published: August 2024

In a study conducted in a metro tunnel, acceleration and displacement sensors were strategically placed along steel rails, track beds, and tunnel walls to capture real-time dynamic responses during train operations. Data were analyzed in the time and frequency domains, focusing on vibration levels and one-third octave bands. The results indicated that peak vibration acceleration significantly decreases from steel rails to tunnel walls, with different vibration frequencies observed at various locations: steel rails (200 Hz–1400 Hz), track beds, and tunnel walls (70 Hz–400 Hz). Cracks notably increase peak acceleration, vibration levels, peak frequency, and steel rail displacement but do not alter the overall vibration trends. Tunnel wall responses show the highest sensitivity to cracks, with a 300% increase in peak frequency, followed by track beds (100%) and steel rails (70%). Vibration levels under one-third octave band processing increased by 12.4% for tunnel walls, 8.8% for track beds, and 2.2% for steel rails. Cracks also caused steel rails’ vertical and lateral displacement to rise by 112% and 53%, respectively. These findings provide valuable insights for vibration reduction and crack repair in long-term subway operations.
Cognitive Bias in Perceptions of Industrialized Housing
Authors: Nolé María Luisa , Montañana Antoni , Barranco-Merino Robi , Higuera-Trujillo Juan Luis , Llinares Carmen
Published: August 2024

Industrialized housing offers potential benefits in efficiency and sustainability compared to traditional construction methods, but public perception often remains negative due to a lack of familiarity. This study explores how prior information and knowledge influence perceptions of industrialized versus traditional housing. An online survey with 267 participants evaluated stimuli composed of an image of a house and a label (industrialized or non-industrialized). Results indicated no significant differences in perception based on visual appearance alone; however, the “industrialized” label was rated less favorably. This effect was more pronounced among those without expertise in industrialized construction. The study also revealed that providing objective prior information improved perceptions of personal and emotional attributes (e.g., cozy, family-friendliness, comfortable, well-being), but did not affect views on construction-specific attributes (e.g., quick-to-build, automated, or modular). These findings underscore the need for neutral and informative communication to counteract negative biases and enhance acceptance of industrialized housing. For the construction industry and policymakers, it is crucial to promote unbiased information and education to overcome resistance due to misconceptions and foster a more positive perception of the product’s features. Addressing concerns and clarifying benefits, these initiatives aim to shift public opinion toward recognizing the value of industrialized housing.
Analysis of Expression and Regulation of AKR1C2 in HPV-Positive and -Negative Oropharyngeal Squamous Cell Carcinoma
Authors: Ziogas Maria , Siefer Oliver , Wuerdemann Nora , Balaji Harini , Gross Elena , Drebber Uta , Klussmann Jens Peter , Huebbers Christian U.
Published: August 2024

Head and Neck Squamous Cell Carcinoma (HNSCC), particularly Oropharyngeal Squamous Cell Carcinoma (OPSCC), is a major global health challenge due to its increasing incidence and high mortality rate. This study investigates the role of aldo-keto reductase 1C2 (AKR1C2) in OPSCC, focusing on its expression, correlation with Human Papillomavirus (HPV) status, oxidative stress status, and clinical outcomes, with an emphasis on sex-specific differences. We analyzed AKR1C2 expression using immunohistochemistry in formalin-fixed, paraffin-embedded tissue samples from 51 OPSCC patients. Additionally, we performed RT-qPCR in cultured HPV16-E6*I and HPV16-E6 overexpressing HEK293 cell lines (p53WT). Statistical analyses were performed to assess the correlation between AKR1C2 expression and patient data. Our results indicate a significant association between increased AKR1C2 expression and higher AJCC classification (p = 0.009) as well as positive HPV status (p = 0.008). Prognostic implications of AKR1C2 varied by sex, whereby female patients with high AKR1C2 expression had better overall survival, whereas male patients exhibited poorer outcomes. Additionally, AKR1C2 expression was linked to HPV status, suggesting a potential HPV-specific regulatory mechanism. These findings underscore the complex interplay among AKR1C2, HPV, and patient sex, highlighting the need for personalized treatment strategies for OPSCC. Targeted inhibition of AKR1C2, considering sex-specific differences, may enhance therapeutic outcomes. Future research should investigate these mechanisms to enhance treatment efficacy.
Identification and Characterization of Multiple Paneth Cell Types in the Mouse Small Intestine
Authors: Timmermans Steven , Wallaeys Charlotte , Garcia-Gonzalez Natalia , Pollaris Lotte , Saeys Yvan , Libert Claude
Published: August 2024

The small intestinal crypts harbor secretory Paneth cells (PCs) which express bactericidal peptides that are crucial for maintaining intestinal homeostasis. Considering the diverse environmental conditions throughout the course of the small intestine, multiple subtypes of PCs are expected to exist. We applied single-cell RNA-sequencing of PCs combined with deep bulk RNA-sequencing on PC populations of different small intestinal locations and discovered several expression-based PC clusters. Some of these are discrete and resemble tuft cell-like PCs, goblet cell (GC)-like PCs, PCs expressing stem cell markers, and atypical PCs. Other clusters are less discrete but appear to be derived from different locations along the intestinal tract and have environment-dictated functions such as food digestion and antimicrobial peptide production. A comprehensive spatial analysis using Resolve Bioscience was conducted, leading to the identification of different PC’s transcriptomic identities along the different compartments of the intestine, but not between PCs in the crypts themselves.
Gut-Brain Axis: Role of Microbiome, Metabolomics, Hormones, and Stress in Mental Health Disorders
Authors: Verma Ankita , Inslicht Sabra S. , Bhargava Aditi
Published: August 2024

The influence of gut microbiome, metabolites, omics, hormones, and stress on general and mental health is increasingly being recognized. Ancient cultures recognized the importance of diet and gut health on the overall health of an individual. Western science and modern scientific methods are beginning to unravel the foundations and mechanisms behind some of the ancient beliefs and customs. The gut microbiome, an organ itself, is now thought to influence almost all other organs, ranging from the brain to the reproductive systems. Gut microbiome, metabolites, hormones, and biological sex also influence a myriad of health conditions that range from mental health disorders, obesity, gastrointestinal disorders, and cardiovascular diseases to reproductive health. Here, we review the history and current understanding of the gut–brain axis bidirectional talk in various mental health disorders with special emphasis on anxiety and depressive disorders, whose prevalence has increased by over 50% in the past three decades with COVID-19 pandemic being the biggest risk factor in the last few years. The vagal nerve is an important contributor to this bidirectional talk, but other pathways also contribute, and most remain understudied. Probiotics containing Lactobacillus and Bifidobacterium species seem to have the most impact on improvement in mental health symptoms, but the challenge appears to be maintaining sustained levels, especially since neither Lactobacillus nor Bifidobacterium can permanently colonize the gut. Ancient endogenous retroviral DNA in the human genome is also linked to several psychiatric disorders, including depression. These discoveries reveal the complex and intricately intertwined nature of gut health with mental health disorders.
Innovative Thin PiG Plates Boost the Luminous Efficacy and Reliability of WLEDs for Vehicles
Authors: Huang Hong-Wei , Huang Chien-Wei , Chen Yi-Chian , Cheng Wei-Chih , Liu Chun-Nien , Chiang Chia-Chin
Published: August 2024

In this study, we demonstrate the high luminous efficacy of 118 lm/W and the high reliability of white LEDs (WLEDs) through 450 °C thermal aging, utilizing four-inch YAG: Ce3+ phosphor-in-glass (PiG) plates designed for vehicle headlights. The sintering process of mixing glass and phosphor typically generates pores, which can scatter light and reduce the luminous efficacy of the fabricated PiG. In this study, we produced four-inch PiG plates under four different fabrication conditions to evaluate their luminous efficacy. Our results revealed that the PiG plate with a thin thickness of 0.08 mm exhibited a 16.83% increase in luminous efficacy compared to the 0.15 mm plate, attributed to reduced light interaction with the pores. Unlike silicone-based phosphor WLEDs, which offer high performance but lower reliability due to the silicone resin’s low transition temperature (150 °C), our novel thin PiG plate achieves high performance and reliability. This advancement suggests that the proposed thin PiG plate could replace traditional silicone-based phosphors, enabling the development of high-quality WLEDs for vehicle headlights in automotive applications.
Navigating the Challenges and Resilience in the Aftermath of the COVID-19 Pandemic in Adolescents with Chronic Diseases: A Scoping Review
Authors: Machado-Kayzuka Giovanna Cristina , Seccarecio Isabela Helena , de Lucca Milena , Neris Rhyquelle Rhibna , Biaggi Leite Ana Carolina Andrade , Alvarenga Willyane de Andrade , De Bortoli Paula Saud , Pinto Manoela Henriques , Nascimento Lucila Castanheira
Published: August 2024

Background/Objectives: The COVID-19 pandemic has profoundly affected the lives of adolescents worldwide, especially those living with chronic diseases. This study aims to explore the impact of the COVID-19 pandemic on the daily lives of adolescents with chronic diseases. Methods: This is a scoping review that follows the guidelines proposed by JBI. Eligibility criteria include articles focusing on adolescents aged 10 to 19 during the COVID-19 pandemic, regardless of chronic diseases. Searches were performed in PUBMED, LILACS, CINAHL, SCOPUS, grey literature, and manual searches in March 2024. Results: This review is composed of 35 articles. The analysis revealed two main categories: (1) Adolescents facing social isolation, school closure, and new family interactions, striving to reinvent themselves, and (2) Chasing the best decision: following up the chronic disease while fighting the COVID-19 pandemic. These categories encompass subcategories highlighting changes in social and family interactions and lifestyle habits. The findings suggest a multifaceted interaction of factors influencing adolescents’ well-being, including improved family bonding, heightened disease management, and increased stress and strains on resources. Conclusions: This review emphasizes the importance of long-term follow-up and social inclusion efforts for adolescents with chronic diseases and their families, addressing their unique needs during public health crises.
Antimicrobial Activity of Gallium(III) Compounds: Pathogen-Dependent Targeting of Multiple Iron/Heme-Dependent Biological Processes
Authors: Choi Seoung-ryoung , Hassan Mohammed A. , Britigan Bradley E. , Narayanasamy Prabagaran
Published: August 2024

Metals play vital roles in biological systems, with iron/heme being essential for cellular and metabolic functions necessary for survival and/or virulence in many bacterial pathogens. Given the rise of bacterial resistance to current antibiotics, there is an urgent need for the development of non-toxic and novel antibiotics that do not contribute to resistance to other antibiotics. Gallium, which mimics iron, has emerged as a promising antimicrobial agent, offering a novel approach to combat bacterial infections. Gallium does not have any known functions in biological systems. Gallium exerts its effects primarily by replacing iron in redox enzymes, effectively inhibiting bacterial growth by targeting multiple iron/heme-dependent biological processes and suppressing the development of drug resistance. The aim of this review is to highlight recent findings on the mechanisms of action of gallium and provide further insights into the development of gallium-based compounds. Understanding the mechanisms underlying gallium’s biological activities is crucial for designing drugs that enhance their therapeutic therapies while minimizing side effects, offering promising avenues for the treatment of infectious diseases.
The Molecular Signature Related to Local Inflammatory and Immune Response in Canine Cutaneous Hypersensitivity Reactions: A Preliminary Study
Authors: Capaccia Camilla , Ciancabilla Francesco , Porcellato Ilaria , Brachelente Chiara , Zerani Massimo , Maranesi Margherita , Guelfi Gabriella
Published: August 2024

Cutaneous hypersensitivity reactions (CHRs) are complex inflammatory skin disorders that affect humans and dogs. This study examined the inflammatory and immune responses leading to skin damage, inflammation, and irritation by investigating gene expression through quantitative PCR (qPCR) and protein localization through the immunohistochemistry (IHC) of specific receptors and molecules involved in CHRs. Formalin-fixed paraffin-embedded (FFPE) samples from canine CHR skin (n = 20) and healthy dog skin (n = 3) were analyzed for expression levels of eight genes, including members of the pattern recognition receptor (PRR) family, CD209 and CLEC4G, the Regakine-1-like chemokine, and acute phase proteins (APPs), LBP-like and Hp-like genes. Additionally, we examined the local involvement of IL-6, Janus Kinase 1 (JAK1), and the signal transducer activator of transcription 3 (STAT3) in the CHR cases. The study demonstrated statistically significant increases in the expression levels of CD209, Hp-like (p < 0.01), LBP-like, Regakine-1-like, and CLEC4G (p < 0.05) genes in CHRs compared to healthy controls. Conversely, IL-6, JAK1, and STAT3 showed no significant difference between the two groups (p > 0.05). Protein analysis revealed JAK1 and STAT3 expression in CHR hyperplastic epithelial cells, dermal fibroblasts, and endothelial cells of small capillaries, indicating a possible involvement in the JAK/STAT pathway in local inflammatory response regulation. Our findings suggest that the skin plays a role in the development of CHRs.
Effects of Mucuna pruriens (L.) DC. and Levodopa in Improving Parkinson’s Disease in Rotenone Intoxicated Mice
Authors: Zaigham Sheher Bano , Paeng Dong-Guk
Published: August 2024

Parkinson’s disease (PD) is the second leading neurodegenerative disease after Alzheimer’s disease. Mucuna pruriens (L.) DC. (MP) is a plant that contains Levodopa (L-DOPA) and has been known to improve the symptoms of PD. In this preliminary study, we investigated the anti-parkinsonian potential of MP to compare the effects of L-DOPA. We first developed an in vivo model of the PD in C57BL/6 male mice using rotenone. A total of twelve mice were used for this experiment. Nine mice were injected with rotenone (28 mg/kg) daily for 28 days. The mice experiments were performed to validate the effectiveness of MP to treat PD. Synthetic L-DOPA in a ratio of 1:20 with MP was used as MP contains 5% L-DOPA by weight in it. MP and L-DOPA were injected for 19 days on a daily basis. Cognitive function was evaluated using beam balance and olfactory tests. Serum analysis was performed using serum enzyme-linked immunosorbent assay (ELISA) analysis test. IL-12, IL-6, and TGF-β 1 were evaluated to validate the PD inducement and treatment. The levels of IL-12, IL-6, and TGF-β1 (p < 0.0001) in the PD mice group were significantly higher than those in the control group. The PD mice also showed higher latencies in beam balance and olfactory tests (p < 0.0001) compared to the control group. Both MP and L-DOPA-treated groups showed alleviation in latencies in beam balance and olfactory tests and decreased neuroinflammation in ELISA analysis (p < 0.001). The results treated by MP and L-DOPA showed insignificant differences in their values (p > 0.05). This proved that the MP and L-DOPA had similar effects in improving the symptoms of PD when used in the ratio of 1:20. Furthermore, both MP and L-DOPA reduced the level of IL-6 and TGF-β1 in this study. It may be inferred that a reduction in the level of IL-6 and TGF-β1 eventually leads to a reduction in the Th17 cells. The pathogenic Th17 is thought to be present in virtually all chronic inflammatory disorders. This can be an interesting area of research in further understanding the immunological effect of MP in ameliorating PD symptoms.
Dihydroavenanthramide D Enhances Skin Barrier Function through Upregulation of Epidermal Tight Junction Expression
Authors: Park Jiye , Shin Jae Young , Kim Daehyun , Jun Seung-Hyun , Jeong Eui Taek , Kang Nae-Gyu
Published: August 2024

Skin barrier dysfunction and thin epidermis are hallmarks of sensitive skin and contribute to premature aging. Avenanthramides are the primary bioactive components of colloidal oatmeal, a commonly used treatment to enhance skin barrier function. This study investigated the relationship between skin barrier function and epidermal characteristics and explored the potential of dihydroavenanthramide D (dhAvD), a synthetic avenanthramide, to improve the skin barrier. We observed a significant correlation between impaired skin barrier function and decreased epidermal thickness, suggesting that a weakened barrier contributes to increased sensitivity. Our in vitro results in HaCaT cells demonstrated that dhAvD enhances keratinocyte proliferation, migration, and tight junction protein expression, thereby strengthening the skin barrier. To mimic skin barrier dysfunction, we treated keratinocytes and full-thickness skin equivalents with IL-4 and IL-13, cytokines that are implicated in atopic dermatitis, and confirmed the downregulation of tight junction and differentiation markers. Furthermore, dhAvD treatment restored the barrier function and normalized the expression of key epidermal components, such as tight junction proteins and natural moisturizing factors, in keratinocytes treated with inflammatory cytokines. In the reconstructed human skin model, dhAvD promoted both epidermal and dermal restoration. These findings suggest that dhAvD has the potential to alleviate skin sensitivity and improve skin barrier function.
Intraductal Papillary Neoplasms of the Bile Duct: Clinical Case Insights and Literature Review
Authors: Toti Luca , Manzia Tommaso Maria , Di Giuliano Francesca , Picchi Eliseo , Tariciotti Laura , Pedini Domiziana , Savino Luca , Tisone Giuseppe , Angelico Roberta
Published: August 2024

Background: Intraductal papillary neoplasms of the bile duct (IPNB) are rare precancerous lesions with implications for the development of cholangiocarcinoma (CCA). Recognizing IPNB and managing its recurrence pose challenges in clinical practice. We present two cases. Case 1: a 60-year-old man presented with an 8 × 8 × 9 cm hepatic cyst initially suspected to be a hydatid cyst. Histology post-resection revealed an IPNB with foci of adenocarcinoma. Despite negative oncologic margins, recurrence occurred eight years later as an rT2N0 lesion. Surgical resection was performed without adjuvant chemotherapy, resulting in the patient’s survival at 48 months post-surgery. Case 2: a 28-year-old female with cognitive impairment was admitted with pulmonary embolism and a liver lesion diagnosed as a simple cyst. Subsequent evaluation revealed adenocarcinoma with local metastases, extensive vascular involvement, and thrombosis. Despite aggressive management, including thrombectomy and chemotherapy, the patient’s condition deteriorated, leading to hepatic failure and eventual demise. Conclusion: IPNB represents a rare premalignant subtype with a propensity for progression to CCA. R0 surgical resection typically offers favorable oncological outcomes with a minimal recurrence risk. Surgical intervention for localized resectable recurrence is both safe and feasible. International registries tracking IPNB recurrence are essential for advancing understanding and optimizing diagnosis, management, and treatment strategies.
Hard and Soft Tissue Facial Landmarks for Mandibular Angle Reduction: A Clinical Study
Authors: Tseng Fei-Fan , Li Yu-Hsuan , Chen Yuan-Wu
Published: August 2024

Background: Square faces, which are influenced by genetic factors and structural features, are considered undesirable among the Asian population. Surgical interventions, such as mandibular angle reduction, aim to alter these characteristics, though complications may arise. We aimed to investigate the morphology of the mandibular angle and masseter muscle thickness using computed tomography (CT) and to analyze hard and soft tissue correlations to enhance surgical outcomes for patients with square faces. Methods: This retrospective clinical study included 100 Taiwanese patients aged 18–50 years. CT was used to analyze key clinical parameters, including bilateral mandibular width, mandibular divergence angle, ramus height, distance from the mandibular angle to the inferior alveolar nerve (IAN), and the thickness of the masseter muscle. Results: Significant correlations were noted between the patients’ physical height and weight, mandibular width, ramus height, masseter thickness, and distance from the angle to the IAN. Males exhibited a significantly longer and thicker ramus height (66.48 ± 4.28 mm), greater masseter thickness (15.46 ± 2.35 mm), and greater safety range for mandibular angle reduction surgery (18.35 ± 3.19 mm) (p < 0.00008). Significant correlations were observed among all parameters, except between mandibular width and gonial angle and the distance from the angle to the IAN and between mandibular divergence and masseter muscle thickness (p > 0.1). Conclusions: Our study highlighted the complex interplay among factors that contribute to square facial morphology. Careful preoperative assessments and customized surgical planning are essential for addressing this multifaceted clinical challenge.