bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of the Complex Interplay Between Nematode-Related lncRNAs and Their Target Genes in Glycine max L.
Authors: Khoei Masoumeh Ahmadi , Karimi Marzieh , Karamian Roya , Amini Sahand , Soorni Aboozar
Published: December 2021

Soybean (Glycine max) is a major plant protein source and oilseed crop. However, plant-parasitic nematodes (PPNs) affect its annual yield. In the current study, in order to better understand the regulation of defense mechanism against PPNs in soybean, we investigated the role of long non-coding RNAs (lncRNAs) in response to two nematode species, Heterodera glycines (SCN: soybean cyst nematode) and Rotylenchulus reniformis (reniform). To this end, two publicly available RNA-seq data sets (SCN data set and RAD: reniform-associated data set) were employed to discover the lncRNAome profile of soybean under SCN and reniform infection, respectively. Upon identification of unannotated transcripts in these data sets, a seven-step pipeline was utilized to sieve these transcripts, which ended up in 384 and 283 potential lncRNAs in SCN data set and RAD, respectively. These transcripts were then used to predict cis and trans nematode-related targets in soybean genome. Computational prediction of target genes function, some of which were also among differentially expressed genes, revealed the involvement of putative nematode-responsive genes as well as enrichment of multiple stress responses in both data sets. Finally, 15 and six lncRNAs were proposed to be involved in microRNA-mediated regulation of gene expression in soybean in response to SNC and reniform infection, respectively. Collectively, this study provides a novel insight into the signaling and regulatory network of soybean-pathogen interactions and opens a new window for further research.
“Carpe Diem?”: Disjunction Effect of Incidental Affect on Intertemporal Choice
Authors: Zhou Lei , Zou Tong , Zhang Lei , Lin Jiao-Min , Zhang Yang-Yang , Liang Zhu-Yuan
Published: December 2021

Incidental affect has an important impact on intertemporal choice (IC). This research aimed to test how positive incidental affect influences IC and its underlying mechanisms. We assumed that positive incidental affect may have a disjunction effect on IC that includes or excludes immediate time. Moreover, we examined the role of time perception for the effect of affect on IC. In Study 1, after undergoing affect priming by video clips, participants completed the IC task using a multiple staircase paradigm. Using Hierarchical Bayesian Modeling, we estimated the discount rate parameter by distinguishing “immediate” and “non-immediate” conditions of IC. The participants’ time perception was also measured. In Study 2, apart from the choice preference of IC, we additionally investigated the differences in the participants’ attention to delay and reward attributes before decision making. The results of the two studies indicated that positive incidental affect leads to longer time perception (Study 1) and prior and more attention to the delay attribute of IC (Study 2), which leads individuals to prefer immediate options in the IC (Studies 1 and 2). Moreover, there is a disjunction effect of affect; in other words, the incidental affect did not influence IC excluding immediate time (Studies 1 and 2). This study improves our understanding of the disjunctive effect and its mechanism of inducing a positive incidental affect on IC and thus provides a new perspective on how related decision making can be improved.
Responsible Leadership Fuels Innovative Behavior: The Mediating Roles of Socially Responsible Human Resource Management and Organizational Pride
Authors: Dong Wenli , Zhong Lifeng
Published: December 2021

Leaders are under increasing pressure to inspire innovative endeavors in responsible ways. However, whether and how responsible leadership can fuel employee innovative behavior remains unknown. Therefore, drawing on social identity theory and social exchange theory, this study aims to investigate the psychological mechanisms underlying the responsible leadership-innovative behavior relationship. Multi-phase data were collected from 280 employees working in Chinese manufacturing firms to test the hypotheses using hierarchical regression analyses and the bootstrap method. The results reveal that responsible leadership is positively related to innovative behavior. Additionally, perceived socially responsible human resource management (HRM) and organizational pride separately and sequentially mediate the responsible leadership-innovative behavior relationship. This study empirically reveals the effectiveness of responsible leadership and sheds new light on the psychological processes through which it facilitates innovative behavior, revealing the generalizability of responsible leadership and innovative behavior in the Chinese context. Moreover, we respond to the call for incorporating leadership theory into HRM research and further advance the existing knowledge on both antecedents and outcomes of socially responsible HRM. For practical guidance, organizations are encouraged to foster innovation through investment in responsible management practices. Research limitations and implications are also discussed.
Defense Mechanisms and Repressive Coping Among Male Breast Cancer Patients
Authors: Weber Rainer , Ehrenthal Johannes C. , Brock-Midding Evamarie , Halbach Sarah , Würstlein Rachel , Kowalski Christoph , Ernstmann Nicole
Published: December 2021

Objectives: The concept of defense mechanisms has undergone extensive revision and expansion since Freud first described these processes. Initially formulated as an unconscious repression of unpleasant memories, with further development focusing on the role of defense mechanisms in the regulation of internal conflicts, the concept shifted and evolved to incorporate the adaptation to external demands, including intrapsychic and interpersonal handling of burden of illness. In addition to defense mechanisms, coping provides another perspective on human adjustment to difficult life events. While there is substantial research on both coping and defense mechanisms in various psychiatric and somatic diseases, including cancer, little is known about defensive regulation, coping, and their interaction in male breast cancer patients.

Methods: The present study is part of the N-Male project conducted between 2016 and 2018 in Germany (Male breast cancer: patients' needs in prevention, diagnosis, treatment, rehabilitation, and follow-up care). Semi-standardized interviews with 27 male breast cancer patients were analyzed with regard to defense mechanisms. In addition, fear of progression and repressive coping was assessed by self-report.

Results: There was considerable variety in levels of defensive functioning as well as repressive coping in our sample. We found no difference in overall levels of defensive functioning between men with vs. without repressive coping. However, patients with repressive coping demonstrated a decopupled association between fear of progression and defensive functioning as compared to patients without repressive coping.

Discussion: The study provides the first evidence of disease processing in male breast cancer patients Knowledge of patients' defense patterns and repressive coping seems promising for better planning targeted intervention strategies.
Mental Disorders and Emotional Competence Among Chinese Adolescents Before and During COVID-19 Pandemic: A Longitudinal Mediation Model
Authors: Shi Wei , Yuan Guangzhe Frank , Hall Brian J. , Liu Xiaoli , Su Ya , Zhao Li , Jia Peng
Published: December 2021

Background: The outbreak of the COVID-19 pandemic has had a profound influence on the mental health and well-being of individuals across the globe. Emotional competence, defined as one's ability to recognize, understand, and manage their emotions, has been found linked with mental health problems (e.g., depression and anxiety) in previous studies. However, there is limited knowledge about the direction of the association between these factors among populations exposed to COVID-19. This study examined the possible mediation relationships between depression, anxiety, emotional competence, and COVID-19 exposure among Chinese adolescents.

Methods: Responses from 7,958 Chinese adolescents who had previously taken part in a two-wave study before (December 23, 2019–January 13, 2020) and during COVID-19 (June 16, 2020–July 8, 2020) were analyzed (51.67% males, mean age = 11.74, SD = 2.15). Structural equation modeling with three covariates (i.e., age, gender, and ethnicity) was used to test the longitudinal mediation relationships between COVID-19 exposure and depression, anxiety via emotional competence.

Results: Results indicated that the prevalence of depression (38.67 to 36.74%) and anxiety (13.02 to 12.77%) decreased from Time 1 to Time 2. The T2 emotional competence significantly mediated the relationship between T2 COVID-19 exposure and T2 anxiety (indirect effect [95% CI] = 0.011 [0.004–0.019], p < 0.05). T2 emotional competence also significantly mediated the relationship between T2 COVID-19 exposure and T2 depression (indirect effect [95% CI] = 0.013 [0.005–0.022], p < 0.05). The results indicated that T2 emotional competence had a significant and negative influence on T2 anxiety (β = −0.266, SE = 0.005, p < 0.001), and T2 depression (β = −0.326, SE = 0.029, p < 0.001).

Conclusions: This longitudinal research study demonstrated the crucial role of emotional competence in influencing the severity of long-term mental health problems, and suggested that emotional competence interventions can be conducted to improve mental well-being among Chinese adolescents exposed to COVID-19.
Influence of Vaccination Characteristics on COVID-19 Vaccine Acceptance Among Working-Age People in Hong Kong, China: A Discrete Choice Experiment
Authors: Wang Kailu , Wong Eliza Lai-Yi , Cheung Annie Wai-Ling , Yau Peter Sen-Yung , Chung Vincent Chi-Ho , Wong Charlene Hoi-Lam , Dong Dong , Wong Samuel Yeung-Shan , Yeoh Eng-Kiong
Published: December 2021

Background: Along with individual-level factors, vaccination-related characteristics are important in understanding COVID-19 vaccine hesitancy. This study aimed to determine the influence of these characteristics on vaccine acceptance to formulate promotion strategies after considering differences among respondents with different characteristics.

Methods: An online discrete choice experiment was conducted among people aged 18–64 years in Hong Kong, China, from 26 to 28 February 2021. Respondents were asked to make choices regarding hypothetical vaccination programmes described by vaccination-related characteristics—the attributes derived from a prior individual interview. Subgroup analysis was performed to identify the differences in vaccination-related characteristics among respondents with different personal characteristics.

Results: A total of 1,773 respondents provided valid responses. The vaccine efficacy and brand were the most important factors affecting acceptance, followed by the exemption of quarantine for vaccinated travelers, safety, venue for vaccination, vaccine uptake of people in their lives, and recommendations by general physicians or government. Frequent exposure to vaccination information on social media has been associated with increasing vaccine refusal. Substantial preference heterogeneity for the attributes was found among people of different ages, incomes, chronic conditions, and previous acceptance of influenza vaccines.

Conclusion: The findings provided evidence to formulate interventions to promote vaccine uptake, including the provision of vaccination at housing estate or workplaces, involvement of general physicians and interpersonal communication in vaccine promotion and information dissemination, and exemption of quarantine for vaccinated people. Moreover, social media is a significant information channel that cannot be neglected in the dissemination of official information.
Predictive Modelling of Susceptibility to Substance Abuse, Mortality and Drug-Drug Interactions in Opioid Patients
Authors: Vunikili Ramya , Glicksberg Benjamin S. , Johnson Kipp W. , Dudley Joel T. , Subramanian Lakshminarayanan , Shameer Khader
Published: December 2021

Objective: Opioids are a class of drugs that are known for their use as pain relievers. They bind to opioid receptors on nerve cells in the brain and the nervous system to mitigate pain. Addiction is one of the chronic and primary adverse events of prolonged usage of opioids. They may also cause psychological disorders, muscle pain, depression, anxiety attacks etc. In this study, we present a collection of predictive models to identify patients at risk of opioid abuse and mortality by using their prescription histories. Also, we discover particularly threatening drug-drug interactions in the context of opioid usage.

Methods and Materials: Using a publicly available dataset from MIMIC-III, two models were trained, Logistic Regression with L2 regularization (baseline) and Extreme Gradient Boosting (enhanced model), to classify the patients of interest into two categories based on their susceptibility to opioid abuse. We’ve also used K-Means clustering, an unsupervised algorithm, to explore drug-drug interactions that might be of concern.

Results: The baseline model for classifying patients susceptible to opioid abuse has an F1 score of 76.64% (accuracy 77.16%) while the enhanced model has an F1 score of 94.45% (accuracy 94.35%). These models can be used as a preliminary step towards inferring the causal effect of opioid usage and can help monitor the prescription practices to minimize the opioid abuse.

Discussion and Conclusion: Results suggest that the enhanced model provides a promising approach in preemptive identification of patients at risk for opioid abuse. By discovering and correlating the patterns contributing to opioid overdose or abuse among a variety of patients, machine learning models can be used as an efficient tool to help uncover the existing gaps and/or fraudulent practices in prescription writing. To quote an example of one such incidental finding, our study discovered that insulin might possibly be interacting with opioids in an unfavourable way leading to complications in diabetic patients. This indicates that diabetic patients under long term opioid usage might need to take increased amounts of insulin to make it more effective. This observation backs up prior research studies done on a similar aspect. To increase the translational value of our work, the predictive models and the associated software code are made available under the MIT License.
A Literature Review and Framework Proposal for Halitosis Assessment in Cigarette Smokers and Alternative Nicotine-Delivery Products Users
Authors: Zanetti Filippo , Zivkovic Semren Tanja , Battey James N. D. , Guy Philippe A. , Ivanov Nikolai V. , van der Plas Angela , Hoeng Julia
Published: December 2021

Halitosis is a health condition which counts cigarette smoking (CS) among its major risk factors. Cigarette smoke can cause an imbalance in the oral bacterial community, leading to several oral diseases and conditions, including intraoral halitosis. Although the best approach to decrease smoking-related health risks is quitting smoking, this is not feasible for many smokers. Switching to potentially reduced-risk products, like electronic vapor products (EVP) or heated tobacco products (HTP), may help improve the conditions associated with CS. To date, there have been few systematic studies on the effects of CS on halitosis and none have assessed the effects of EVP and HTP use. Self-assessment studies have shown large limitations owing to the lack of reliability in the participants' judgment. This has compelled the scientific community to develop a strategy for meaningful assessment of these new products in comparison with cigarettes. Here, we compiled a review of the existing literature on CS and halitosis and propose a 3-layer approach that combines the use of the most advanced breath analysis techniques and multi-omics analysis to define the interactions between oral bacterial species and their role in halitosis both in vitro and in vivo. Such an approach will allow us to compare the effects of different nicotine-delivery products on oral bacteria and quantify their impact on halitosis. Defining the impact of alternative nicotine-delivery products on intraoral halitosis and its associated bacteria will help the scientific community advance a step further toward understanding the safety of these products and their potentiall risks for consumers.
Contesting Sustainability of Mega-Events in Chinese Metropolises: A Narrative and Practise Review
Authors: Li Lingyue
Published: December 2021

Since the Rio Summit, sustainability has been enshrined in the official claims of mega-events, and scholars' sustainability concerns over mega-events persist. This research is one of the few contextualising the debates in China where mega-events have become prevalent and contributed to metropolitan boosterism since the 1990s. It argues that the ill-defined, ambiguous meaning of sustainability leaves room for host cities to discursively strike a balance between environment protection, social equity and economic growth, but unavoidably leads to paradoxes of sustainability in actual practises. The argument is supported by a longitudinal study on 12 major mega-events held or to be held in Chinese metropolises. Narratively, Chinese government's articulation of sustainability declared by international organisations of top mega-events has evolved technocratic and inclusive. Practically, amongst the three variants of sustainability, economic sustainability, and environmental sustainability appear to work better than social sustainability. Findings of this research call for clarified narratives and concrete measures to guide mega-event practises.
Imagine That! Mental Training for Children With Developmental Coordination Disorder
Authors: Scott Matthew W. , Wood Greg , Holmes Paul S. , Marshall Ben , Williams Jacqueline , Wright David J.
Published: December 2021

Movement is important for children’s health and well-being. Most children find it easy to learn to move but children with developmental coordination disorder (DCD) find it hard. It can be tricky for them to plan and control their movements. DCD affects 1 in every 20 children. It makes important tasks difficult, like getting dressed or playing games and sports. Scientists have found that children with DCD have different activity in some brain areas compared to other children. Mental training can increase activity in these areas of the brain. One type of mental training is motor imagery, which involves imagining doing movements. Another type of mental training is action observation, which involves carefully watching how people make certain movements. These techniques can help children with DCD get better at moving. This means that doing mental training might help make life easier for children with DCD.
Measuring Changes in Perceptions of Access to Pet Support Care in Underserved Communities
Authors: Hawes Sloane M. , Hupe Tess M. , Winczewski Jordan , Elting Kaitlyn , Arrington Amanda , Newbury Sandra , Morris Kevin N.
Published: December 2021

Understanding social, economic, and structural barriers to accessing pet care services is important for improving the health and welfare of companion animals in underserved communities in the U.S. From May 2018-December 2019, six questions from the validated One Health Community Assessment were used to measure perceptions of access to pet care in two urban and two rural zip codes. One urban and one rural community received services from a pet support outreach program (Pets for Life), while the other served as a comparison community. After propensity score matching was performed to eliminate demographic bias in the sample (Urban = 512 participants, Rural = 234 participants), Generalized Estimating Equations were employed to compare the six measures of access to pet care between the intervention and comparison communities. The urban community with the Pets for Life intervention was associated with a higher overall measure of access to pet care compared to the urban site that did not have the Pets for Life intervention. When assessing each of the six measures of access to care, the urban community with the Pets for Life intervention was associated with higher access to affordable pet care options and higher access to pet care service providers who offer payment options than the community without the Pets for Life intervention. Further analyses with a subset of Pets for Life clients comparing pre-intervention and post-intervention survey responses revealed statistically significant positive trends in perceptions of two of the six measures of access to pet care. This study provides evidence that community-based animal welfare programming has the potential to increase perceptions of access to pet support services.
The Development of a Flight Test Platform to Study the Body Freedom Flutter of BWB Flying Wings
Authors: Shi Pengtao , Liu Feng , Gu Yingsong , Yang Zhichun
Published: December 2021

A flight test platform is designed to conduct an experimental study on the body freedom flutter of a BWB flying wing, and a flight test is performed by using the proposed platform. A finite element model of structural dynamics is built, and unsteady aerodynamics and aeroelastic characteristics of the flying wing are analyzed by the doublet lattice method and g-method, respectively. Based on the foregoing analyses, a low-cost and low-risk flying-wing test platform is designed and manufactured. Then, the ground vibration test is implemented, and according to its results, the structural dynamics model is updated. The flight test campaign shows that the body freedom flutter occurs at low flight speed, which is consistent with the updated analytical result. Finally, an active flutter suppression controller is designed using a genetic algorithm for the developed flying wing for future tests, considering the gains and sensor location as design parameters. The open- and closed-loop analyses in time- and frequency-domain analyses demonstrate that the designed controller can improve the instability boundary of the closed-loop system effectively.
New Intrinsically Thermostable Xylanase Improves Broilers’ Growth Performance, Organ Weights, and Affects Intestinal Viscosity and pH
Authors: Van Hoeck Veerle , Papadopoulos Georgios A. , Giannenas Ilias , Lioliopoulou Styliani , Tsiouris Vasilios , Mantzios Tilemachos , Kiskinis Konstantinos , Grivas Ioannis , Gonzalez Sanchez Antonio Luis , Vasanthakumari Bindhu Lakshmibai , Fortomaris Paschalis , Morisset Dany
Published: December 2021

Exogenous carbohydrases are commonly added to monogastric animal feed to degrade non-starch polysaccharides (NSP) to improve zootechnical performance and nutrient digestion. In the current study, the effects of dietary supplementation of a new, intrinsically thermostable, monocomponent xylanase on intestinal parameters, zootechnical performance and carcass traits of broilers were evaluated. A total of 720 1-day-old broilers were randomly allotted to four wheat-based diets with different levels of xylanase (T1: control; T2: 30,000 U/g; T3: 45,000 U/g; T4: 90,000 U/g) and fed from 1 to 35 days of age. The data showed that xylanase supplementation reduced the intestinal viscosity significantly, especially in the ileum. Duodenum and cecum pH was not influenced by xylanase supplementation, while T3 treatment showed the lowest pH value in jejunum and ileum. Xylanase supplementation reduced significantly feed conversion ratio (FCR) without affecting feed intake (FI). T2 treatment exhibited a higher body weight gain (BWG) compared the other treatments. Live weight, carcass weight, legs, and liver weight were significantly higher in T3 and T4 treatments compared to T1. No significant differences on foot pad lesions were observed among the four treatments. In conclusion, dietary supplementation of this new xylanase significantly improves intestinal tract viscosity and affects beneficially broilers’ performance and carcass traits.
Agro-Morphological, Biochemical and Antioxidant Characterization of a Tunisian Chili Pepper Germplasm Collection
Authors: Lahbib Karima , Dabbou Samia , Bnejdi Fethi , Pandino Gaetano , Lombardo Sara , El Gazzah Mohamed , El Bok Safia
Published: December 2021

Pepper species have been described as being highly sensitive to climate change. Here, we discuss the variability of the agro-morphological and phytochemical responses of pepper cultivars in the context of ongoing climate changes during seven stages of maturity, including heat stress. The effects and interactions were calculated to determine the source of variation according to rising temperature. Capsaicin content (CAP), total phenolic (TPC) and flavonoid (TFC) levels and antioxidant activity (AA) were also determined at different harvest times (at 10, 25, 40, 55, 70, 85 and 100 days after anthesis, DAA). Agro-morphological data showed that the highest variation was recorded for fruit traits compared to flower and plant ones. In particular, calyx shape margin, calyx annular constriction, fruit shape at blossom end and fruit size had a significant impact on the morphologic diversity among accessions. Levels of bioactive compounds and antioxidant activity depended on the genotype and the harvest time. TPC and AA increased at 100 DAA, while TFC were highly detected at the early harvest. Principal component analysis (PCA) allowed us to separate three clusters with well-defined biochemical traits. In particular, regardless of harvest time, Baklouti Chébika, Baklouti Sbikha and Chaabani accessions presented higher levels of TPC, TFC and AA regardless of the considered harvest time. In conclusion, high genetic variability was noted within the analyzed pepper germplasm, thus suggesting the need for major consideration of both agro-morphological and biochemical traits for pepper breeding programs. The current research was conducted to facilitate better management under high-stress conditions due to global warming
Design and Simulation of a Garlic Seed Metering Mechanism
Authors: Guo Huiping , Cao Yazhou , Song Wenyuan , Zhang Jiao , Wang Changlin , Wang Chensi , Yang Fuzeng , Zhu Lin
Published: December 2021

According to the agronomic requirements of garlic sowing, the garlic morphology is studied and a garlic seed metering mechanism with excellent seeding performance is designed. Based on this design, a new garlic seeding machine with an adjustable-size seeding device is developed to realize efficient single-seed metering and seeding of different varieties of garlic. Further, the design scheme of the garlic seeder prototype is established, with the key components of the garlic seeding being designed on the basis of the garlic seeding mechanism. To achieve garlic single-seed metering for different varieties of garlic, the optimal adjustment size of the garlic seed metering device is determined through discrete element simulation analysis. A field experiment confirms the effectiveness of applying the proposed garlic planter to field sowing in terms of the metrics of missing seed and multiple seed rates. The results of the discrete element simulation test reveal that an adjustment size of 40 mm yields the best single-seed metering performance. At an operating speed of 15–35 rpm, the metering device can achieve more than an 80% qualification rate of single-seed metering, with a unit speed of 0.628–1.465 m/s. Thus, the developed garlic seeding device meets the requirements of precision sowing in China and can effectively realize the mechanized planting of garlic.
Physiological Quality of Swingle Citrumelo Seed after Refrigerated Storage of Fruits and Seeds
Authors: Morelli Marilia , Azevedo Fernando Alves de , de Souza Ana Julia Borim , Martinelli Rodrigo , da Conceição Patrícia Marluci
Published: December 2021

For citrus, there is no definition of the ideal fruit harvesting point for seed extraction. Some studies have shown the positive effect of postharvest refrigerated storage of fruits to obtain quality seeds. This study aimed to evaluate the influence of the fruit maturation stage on the germination of Swingle citrumelo (CS) seeds after the refrigerated storage of fruits and seeds. CS fruits were harvested at 180 days after anthesis (DAA); 210 DAA and 240 DAA. Fruits and seeds were stored in cold chamber for 0, 15, 30, and 45 days. Physicochemical analyses were performed on fruits (colour, soluble solids, acidity and ratio) and seeds (water content, germination, and emergence). Germination of seeds extracted from fruits harvested at 180, 210, and 240 DAA, without refrigerated storage, showed a positive correlation with colour index. The refrigerated storage of fruits and seeds extracted from fruits harvested at 210 DAA, increased the seed germination rate. Peel colour (IC) was correlated positively with the germination of Swingle citrumelo seeds. The results of this study revealed that the absence of refrigerated storage, harvesting Swingle citrumelo fruits, with ripe characteristics (end of harvest), results in a high seed germination rate. On the other hand, refrigerated storage of fruits and seeds extracted from fruits harvested at mid-harvest increases the seed germination rate.
Fungal Endophytes of Vitis vinifera—Plant Growth Promotion Factors
Authors: Kulišová Markéta , Vrublevskaya Maria , Lovecká Petra , Vrchotová Blanka , Stránská Milena , Kolařík Miroslav , Kolouchová Irena
Published: December 2021

Endophytes are microorganisms that live asymptomatically inside plant tissues. They are beneficial to their host in many aspects, especially as a defense against foreign phytopathogens through the production of a variety of metabolites. These substances can serve as sources of new natural products for medicinal, agricultural, and industrial purposes. This article is focused on endophytic fungi from Vitis vinifera. The purpose of the research was their isolation and identification during the Vitis vinifera growing season. Subsequently, the isolates were tested for the production of biotechnologically interesting metabolites (siderophores, antioxidants, and antifungal compounds). In total, 24 endophytic fungi were isolated, the most represented genus was Cladosporium sp. The results of the test for antioxidant and antifungal properties, as well as siderophore production, have shown that the population of Vitis vinifera endophytic microscopic fungi could serve as a promising source of metabolites with a wide range of applications.
Next Steps for Conservation Agriculture
Authors: Landers John N. , de Freitas Pedro Luiz , de Oliveira Mauricio Carvalho , da Silva Neto Sebastião Pedro , Ralisch Ricardo , Kueneman Eric Alan
Published: December 2021

The origins, history, and recent advances in Conservation Agriculture (CA) are reported. CA is now practiced worldwide on some 200 million hectares, important for mitigating climate change and ensuring food security. Its bedrock is Zero Tillage (ZT) with crop rotation and retention of crop residues. CA approaches Organic Agriculture (OA) when coupled to biological control providing opportunity for OA to become truly sustainable. Ley Farming (LF) and agroforestry with ZT are important for carbon sequestration and land use intensification. Hidden cost: each ton of carbon immobilizes 83 kg of N, 29 kg of P, and 14 kg of S. Industry-backed Regenerative Agriculture (RA) variants have no scientific definition, but generally adopt CA. Sustainable, profitable, and compatible new technologies are emerging and CA needs to embrace them to present a holistic, sustainable package to the farmer. How? A single definition for agricultural sustainability via a multi-stakeholder world congress would standardize certification and de-confuse the market. RA describes exactly what CA does for soil health and all farmers need to unite around a new “Combined Regenerative Agriculture” (CRA) to lobby for adequate payments for environmental services. Expansion of CA is critical for world sustainability. Many gaps and constraints exist, especially for smallholders.
The Comparison of Juvenile Hormone and Transcriptional Changes between Three Different Juvenile Hormone Analogs Insecticides on Honey Bee Worker Larval’s Development
Authors: Luo Jie , Liu Sheng , Hou Jiangan , Chen Lichao , Li Honghong , Liao Shucheng , Tan Qimei , Yang Taiyi , Yi Guoqiang , Zhang Fu , Li Xuesheng
Published: December 2021

Juvenile hormones (JHs) play a crucial role in the development of honey bee (Apis mellifera) worker larvae. Juvenile hormone analogs (JHAs), insecticides widely used in pest control, have been reported to affect the health and survival of honey bee worker larvae. However, the molecular mechanisms of JHAs in the honey bee remain unclear. In this study, we treated honey bee worker larvae with pyriproxyfen, fenoxycarb, and methoprene, three different JHAs. We monitored the changes in the transcription of genes encoding major JH response enzymes (CYP15A1, CYP6AS5, JHAMT, and CHT1) using RT-qPCR and analyzed the transcriptome changes in worker larvae under JHA stress using RNA-seq. We found that the enrichment pathways differed among the treatment groups, but the classification of each pathway was generally the same, and fenoxycarb affected more genes and more pathways than did the other two JHAs. Notably, treatment with different JHAs in the honey bee changed the JH titers in the insect to various extents. These results represent the first assessment of the effects of three different JHAs on honey bee larvae and provide a new perspective and molecular basis for the research of JH regulation and JHA toxicity in the honey bee.
Two-Stage Spatiotemporal Time Series Modelling Approach for Rice Yield Prediction & Advanced Agroecosystem Management
Authors: Rathod Santosha , Saha Amit , Patil Rahul , Ondrasek Gabrijel , Gireesh Channappa , Anantha Madhyavenkatapura Siddaiah , Rao Dhumannatarao Venkata Krishna Nageswara , Bandumula Nirmala , Senguttuvel Ponnuvel , Swarnaraj Arun Kumar , Meera Shaik N. , Waris Amtul , Jeyakumar Ponnuraj , Parmar Brajendra , Muthuraman Pitchiahpillai , Sundaram Raman Meenakshi
Published: December 2021

A robust forecast of rice yields is of great importance for medium-to-long-term planning and decision-making in cereal production, from regional to national level. Incorporation of spatially correlated adjacent effects in forecasting models in general, results in accurate forecast. The Space Time Autoregressive Moving Average (STARMA) is the most popular class of model in linear spatiotemporal time series modelling. However, STARMA cannot process nonlinear spatiotemporal relationships in datasets. Alternately, Time Delay Neural Network (TDNN) is a most popular machine learning algorithm to model the nonlinear pattern in data. To overcome these limitations, two-stage STARMA approach was developed to predict rice yield in some of the most intensive national rice agroecosystems in India. The Mean Absolute Percentage Errors value of proposed STARMA-II approach is lower compared to Autoregressive Moving Average (ARIMA) and STARMA model in all examined districts, while the Diebold-Mariano test confirmed that STARMA-II model is significantly different from classical approaches. The proposed STARMA-II approach is promising alternative to classical linear and nonlinear spatiotemporal time series models for estimating mixed linear and nonlinear patterns and can be advanced tool for mid-to-long-term sustainable planning and management of crop yields and patterns in agroecosystems, i.e., food supply and demand from local to regional levels.
Transcriptomic Responses of Fall Armyworms (Spodoptera frugiperda) Feeding on a Resistant Maize Inbred Line Xi502 with High Benzoxazinoid Content
Authors: Malook Saif ul , Liu Xiao-Feng , Ma Caiyan , Qi Jinfeng , Liu Wende , Zhou Shaoqun
Published: December 2021

The fall armyworm (Spodoptera frugiperda) is a devastating invasive insect herbivore. Its success on its preferred host plant, maize (Zea mays), is supported by numerous specialized detoxification mechanisms that suppress the defense responses of maize. In this study, we used a resistant Chinese maize cultivar, Xi502, which showed slower growth and lower yield-related phenotypes compare with maize inbred line B73. Comparative transcriptomic analyses demonstrated that B73-fed fall armyworm larvae have a significantly faster transcriptomic re-configuration toward maturation compared to their siblings fed with Xi502 leaves, whereas a number of putative aromatic breakdown -related DEGs were specifically induced when feeding on Xi502. Targeted metabolomic quantification demonstrated that Xi502 contains significantly higher levels of various benzoxazinoid compounds. Artificial feeding with the structural analog of a benzoxazinoid compound preferentially accumulated in Xi502 demonstrated a significant growth inhibition effect on FAW larvae. These results provide important genetic material and preliminary evidence for further dissection of the FAW-resistance mechanism in maize.
Impact of Long-Term Storage on Alfalfa Leaf and Stem Silage Characteristics
Authors: Sikora Miranda C. , Hatfield Ronald D. , Kalscheur Kenneth F.
Published: December 2021

Fractionation of alfalfa (Medicago sativa L.) into leaves and stems can reduce cutting frequency while producing a high-value feedstuff. A difficulty with fractionation is the higher moisture content at ensiling due to the inability to wilt leaves without substantial dry matter loss or financial cost. To evaluate whether the silage quality of fractionated alfalfa is competitive with conventional methods under long-term storage conditions, high-moisture leaves (250–280 g·kg−1 dry matter, DM) and stems (190–240 g·kg−1 DM) were ensiled for 21 and 350 days and compared to wilted whole-plant silage for two alfalfa developmental stages. At 21 days, leaf and whole-plant silage fermentation characteristics indicated suitable lactic acid bacterial fermentation through decreased pH, high lactic acid–to-acetic acid ratio, and a lack of clostridial fermentation. At 350 days, leaf silage fermentation and nutritional quality decreased due to sustained proteolysis, but true protein still exceeded that of whole-plant silage. High-moisture stem fractions fermented poorly; at 21 days, stage 3 stems had significant amounts of butyric acid, while stage 5 stems became clostridial at 350 days. Long-term storage of high-moisture leaf silages can produce good-quality silage despite exceeding moisture contents typically recommended for alfalfa, while wilting is required for stem silages.
Nutritional Value of Whole Maize Kernels from Diverse Endosperm Types and Effects on Rheological Quality
Authors: Álvarez-Iglesias Lorena , Malvar Rosa Ana , Garzón Raquel , Rosell Cristina M. , Revilla Pedro
Published: December 2021

Maize’s (Zea mays L.) nutrient content can be modified through selection. The objective of this study was to assess the nutritive value of 13 maize hybrids from four endosperm types, and the relationship between nutrient concentration, agronomic and rheological value. The hybrids were evaluated in two locations of Northwestern Spain over two years. There was genetic diversity among endosperm types and genotypes for all nutrients, with significant environmental effects, but few genotype × environment interactions. Flint hybrids had the highest protein and lipid content. The mutant wx1 significantly increased protein and reduced lipid, and both wx1 and o2 reduced ash and starch content and increased total fiber. Variability among hybrids within the wx1 endosperm was significant for most nutrients. Correlations between nutrients were rarely significant, implying that protein and lipid can be improved independently. Protein and lipid were negatively correlated with grain yield and plant height. However, improving nutrient content could alter agronomic performance, as nutrients had significant negative effects on rheological factors, particularly protein and lipid, which had negative effects on whole meal and on bread characteristics. Therefore, nutrient content can be improved in maize, but negative effects on agronomic and quality have to be taken into account.
Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation
Authors: Rivera del Alamo Maria Montserrat , Reilas Tiina , Lukasik Karolina , Galvão Antonio M. , Yeste Marc , Katila Terttu
Published: December 2021

Intrauterine devices (IUDs) are used in mares to suppress oestrous behaviour, but the underlying mechanism is yet to be elucidated. The presence of an embryo or an IUD prevents cyclooxygenase-2 (COX-2) and, subsequently, prostaglandin (PG) release and luteolysis. However, inflammation may also be involved. Endometrial inflammatory markers in uterine lavage fluid were measured on Day 10 (EXP 1, n = 25) and Day 15 (EXP 2, n = 27) after ovulation in inseminated mares, non-pregnant or pregnant, and in mares in which a small plastic sphere had been inserted into the uterus 4 (EXP 1) or 3 days (EXP 2) after ovulation. Uterine lavage fluid samples were analysed for nitric oxide (NO), prostaglandin E2 (PGE2) (only EXP 1), prostaglandin F2α (PGF2α), inhibin A and cytokines, and blood samples for progesterone and oestradiol. On Day 10, the concentration of PGF2α was lower (p < 0.05) in the IUD group than in pregnant mares. The concentration of the modulatory cytokine IL-10 was significantly higher in the IUD group in comparison to non-pregnant mares, and inhibin A was significantly higher in IUD mares than in the pregnant counterparts on Day 15. The results suggest that the presence of IUD causes endometrial inflammation which is at a resolution stage on Day 15.
The Occurrence of Quill Mites (Arachnida: Acariformes: Syringophilidae) on Bee-Eaters (Aves: Coraciiformes: Meropidae: Merops) of Two Sister Clades
Authors: Skoracki Maciej , Kosicki Jakub Z. , Sikora Bozena , Töpfer Till , Hušek Jan , Unsöld Markus , Hromada Martin
Published: December 2021

We studied the quill mite fauna of the family Syringophilidae, associated with bee-eaters. We examined 273 bird specimens belonging to nine closely related species of the genus Merops, representing two phylogenetic sister clades of a monophyletic group. Our examination reveals the presence of two species of the genus Peristerophila, as follows: (1) a new species Peristerophila mayri sp. n. from Merops viridis in the Philippines, M. leschenaulti in Nepal and Sri Lanka, and M. orientalis in Sri Lanka; and (2) P. meropis from M. superciliosus in Tanzania and Egypt, M. persicus in Sudan, Tanzania, Liberia, Senegal, Kenya, and D.R. Congo, M. ornatus in Papua New Guinea, M. philippinus in Thailand, Indonesia and Sri Lanka, and M. americanus in the Philippines. The prevalence of host infestations by syringophilid mites varied from 3.1 to 38.2%. The distribution of syringophilid mites corresponds with the sister clade phylogenetic relationships of the hosts, except for P. meropis associated with Merops americanus. Possible hypotheses for the host lineage shift are proposed.
Effects of Probiotics on Growth, Survival, Water Quality and Disease Resistance of Red Hybrid Tilapia (Oreochromis spp.) Fingerlings in a Biofloc System
Authors: Zabidi Aimi , Yusoff Fatimah Md , Amin Nurul , Yaminudin Nur Jasmin Mohd , Puvanasundram Puvaneswari , Karim Murni Marlina Abd
Published: December 2021

Biofloc technology has shown positive effects in aquaculture, especially on the growth performance of cultured animals. The aims of this study were to evaluate the effects of adding different probiotic strains in a biofloc system on the growth performance and disease resistance of red hybrid tilapia (Oreochromis spp.). Three different probiotics (Lysinibacillus fusiformis SPS11, Bacillus amyloliquefaciens L9, and Enterococcus hirae LAB3), commercial probiotics (MG1) and a mixed probiotics (MP) combining all three strains were used in this study. The in vitro assay results showed that the mixed probiotic (MP) was able to inhibit the growth of Streptococcus agalactiae and Streptococcus iniae significantly compared to the single and commercial probiotic. The efficacy of MP was further tested in in vivo tilapia culture challenged with S. agalactiae. The best specific growth rate (3.73 ± 0.23% day−1) and feed conversion ratio (0.76 ± 0.04) were recorded in the group of biofloc with addition of MP. After being challenged with S. agalactiae, the group of biofloc with MP had significantly higher survival (83 ± 1.43%) compared to the other groups. Furthermore, the nitrogen concentration (NO2-N and NH4-N) was significantly lower in all the biofloc groups compared to the control. Hence, the addition of probiotics was able to provide beneficial effects to red hybrid tilapia culture in the biofloc system.
Short-Term Adaptation of Dairy Cattle Production Parameters to Individualized Changes in Dietary Top Dress
Authors: Price Tanner P. , Souza Vinícius C. , Liebe Douglas M. , Elett Mark D. , Davis Ty C. , Gleason Claire B. , Daniels Kristy M. , White Robin R.
Published: December 2021

Immediate and short-term changes in diet composition can support individualized, real-time interventions in precision dairy production systems, and might increase feed efficiency (FE) of dairy cattle in the short-term. The objective of this study was to determine immediate and short-term effects of changes in diet composition on production parameters of dairy cattle fed varying amounts of top dressed commodities. A 4 × 4 replicated Latin square design was used to evaluate responses of twenty-four Holstein cows fed either no top dress (Control) or increasing amounts of: corn grain (CG), soybean meal (SBM), or chopped mixed grass hay (GH) top dressed on a total mixed ration (TMR) over four, 9-day periods. Throughout each period, top dressed commodities were incrementally increased, providing 0% to 20% of calculated net energy of lactation (NEL) intake. Measured production responses were analyzed for each 9-d period using a mixed-effects model considering two different time ranges. Samples collected from d 3 and 4 and from d 7 and 8 of each period were averaged and used to reflect “immediate” vs. “short-term” responses, respectively. In the immediate response time frame, control fed cows had lower milk yield, milk fat yield, and milk true protein yield than CG and SBM supplemented animals but similar responses to GH supplemented animals. Milk fat and protein percentages were not affected by top dress type in the immediate term. In the short-term response time-frame, GH supplemented animals had lower DMI and milk fat yield than all other groups. Control and GH supplemented cows had lower milk yield than CG and SBM fed cows. In the immediate response time frame, FE of SBM supplemented cows was superior to other groups. In the short-term time frame, FE of GH and SBM groups was improved over the control group. Results suggest that lactating dairy cows show rapid performance responses to small (<20% NEL) changes in dietary composition, which may be leveraged within automated precision feeding systems to optimize efficiency of production. Before this potential can be realized, further research is needed to examine integration of such strategies into automatic feeding systems and downstream impacts on individual animal FE and farm profitability.