bluuebunny/crossref_metadata_2025 huggingface dataset:
Circuitous Path to Live Donor Liver Transplantation from the Coordinator’s Perspective
Authors: Lin Hui-Ying , Ho Cheng-Maw , Hsieh Pei-Yin , Lin Min-Heuy , Wu Yao-Ming , Ho Ming-Chih , Lee Po-Huang , Hu Rey-Heng
Published: November 2021

Background: The live donor liver transplantation (LDLT) process is circuitous and requires a considerable amount of coordination and matching in multiple aspects that the literature does not completely address. From the coordinators’ perspective, we systematically analyzed the time and risk factors associated with interruptions in the LDLT process. Methods: In this retrospective single center study, we reviewed the medical records of wait-listed hospitalized patients and potential live donors who arrived for evaluation. We analyzed several characteristics of transplant candidates, including landmark time points of accompanied live donation evaluation processes, time of eventual LDLT, and root causes of not implementing LDLT. Results: From January 2014 to January 2021, 417 patients (342 adults and 75 pediatric patients) were enrolled, of which 331 (79.4%) patients completed the live donor evaluation process, and 205 (49.2%) received LDLT. The median time from being wait-listed to the appearance of a potential live donor was 19.0 (interquartile range 4.0–58.0) days, and the median time from the appearance of the donor to an LDLT or a deceased donor liver transplantation was 68.0 (28.0–188.0) days. The 1-year mortality rate for patients on the waiting list was 34.3%. Presence of hepatitis B virus, encephalopathy, and hypertension as well as increased total bilirubin were risk factors associated with not implementing LDLT, and biliary atresia was a positive predictor. The primary barriers to LDLT were a patient’s critical illness, donor’s physical conditions, motivation for live donation, and stable condition while on the waiting list. Conclusions: Transplant candidates with potential live liver donors do not necessarily receive LDLT. The process requires time, and the most common reason for LDLT failure was critical diseases. Aggressive medical support and tailored management policies for these transplantable patients might help reduce their loss during the process.
Return Based Risk Measures for Non-Normally Distributed Returns: An Alternative Modelling Approach
Authors: Samunderu Eyden , Murahwa Yvonne T.
Published: November 2021

Developments in the world of finance have led the authors to assess the adequacy of using the normal distribution assumptions alone in measuring risk. Cushioning against risk has always created a plethora of complexities and challenges; hence, this paper attempts to analyse statistical properties of various risk measures in a not normal distribution and provide a financial blueprint on how to manage risk. It is assumed that using old assumptions of normality alone in a distribution is not as accurate, which has led to the use of models that do not give accurate risk measures. Our empirical design of study firstly examined an overview of the use of returns in measuring risk and an assessment of the current financial environment. As an alternative to conventional measures, our paper employs a mosaic of risk techniques in order to ascertain the fact that there is no one universal risk measure. The next step involved looking at the current risk proxy measures adopted, such as the Gaussian-based, value at risk (VaR) measure. Furthermore, the authors analysed multiple alternative approaches that do not take into account the normality assumption, such as other variations of VaR, as well as econometric models that can be used in risk measurement and forecasting. Value at risk (VaR) is a widely used measure of financial risk, which provides a way of quantifying and managing the risk of a portfolio. Arguably, VaR represents the most important tool for evaluating market risk as one of the several threats to the global financial system. Upon carrying out an extensive literature review, a data set was applied which was composed of three main asset classes: bonds, equities and hedge funds. The first part was to determine to what extent returns are not normally distributed. After testing the hypothesis, it was found that the majority of returns are not normally distributed but instead exhibit skewness and kurtosis greater or less than three. The study then applied various VaR methods to measure risk in order to determine the most efficient ones. Different timelines were used to carry out stressed value at risks, and it was seen that during periods of crisis, the volatility of asset returns was higher. The other steps that followed examined the relationship of the variables, correlation tests and time series analysis conducted and led to the forecasting of the returns. It was noted that these methods could not be used in isolation. We adopted the use of a mosaic of all the methods from the VaR measures, which included studying the behaviour and relation of assets with each other. Furthermore, we also examined the environment as a whole, then applied forecasting models to accurately value returns; this gave a much more accurate and relevant risk measure as compared to the initial assumption of normality.
Land Use Increases the Correlation between Tree Cover and Biomass Carbon Stocks in the Global Tropics
Authors: Bhan Manan , Gingrich Simone , Matej Sarah , Fritz Steffen , Erb Karl-Heinz
Published: November 2021

Tree cover (TC) and biomass carbon stocks (CS) are key parameters for characterizing vegetation and are indispensable for assessing the role of terrestrial ecosystems in the global climate system. Land use, through land cover change and land management, affects both parameters. In this study, we quantify the empirical relationship between TC and CS and demonstrate the impacts of land use by combining spatially explicit estimates of TC and CS in actual and potential vegetation (i.e., in the hypothetical absence of land use) across the global tropics (~23.4° N to 23.4° S). We find that land use strongly alters both TC and CS, with stronger effects on CS than on TC across tropical biomes, especially in tropical moist forests. In comparison to the TC-CS correlation observed in the potential vegetation (biome-level R based on tropical ecozones = 0.56–0.90), land use strongly increases this correlation (biome-level R based on tropical ecozones = 0.87–0.94) in the actual vegetation. Increased correlations are not only the effects of land cover change. We additionally identify land management impacts in closed forests, which cause CS reductions. Our large-scale assessment of the TC-CS relationship can inform upcoming remote sensing efforts to map ecosystem structure in high spatio-temporal detail and highlights the need for an explicit focus on land management impacts in the tropics.
Expanding the Protection of Children’s Rights towards a Dignified Life: The Emerging Jurisprudential Developments in the Americas
Authors: Fuentes Alejandro , Vannelli Marina
Published: November 2021

The Inter-American Court of Human Rights (IACrtHR) has developed in recent years an innovative jurisprudence that has integrated the entity and extension of States’ obligations regarding children’s rights—as established in Article 19 ACHR—through the evolutive, dynamic, and effective interpretation of the American Convention on Human Rights (ACHR). In fact, by acknowledging the existence of an international corpus juris for the protection of children’s rights, the Court has examined this provision in the light of instruments enshrined within the corpus juris, such as the UN Convention on the Rights of the Child. This process of normative integration was not only limited to the application of international instruments adopted outside of the Inter-American system, but also includes internal references to interconnected rights recognised within the American Convention. Consequently, by analysing the scope of Article 19 ACHR in the light of Article 4 ACHR (right to life) and the corpus juris for the protection of children, the Inter-American Court has further expanded the protection of children’s rights towards the protection of the right to a dignified life. While focusing on the landmark jurisprudence developed by IACrtHR, this paper seeks to unveil the hermeneutical paths undertaken by the regional tribunal in connection with the systemic integration of Article 19 ACHR. In particular, it focuses on the emerging jurisprudential development of positive obligations upon States Members regarding the effective protection of children’s right to a dignified existence.
Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV
Authors: Lv Changjie , Zhao Ya , Jiang Lili , Zhao Li , Wu Chao , Hui Xianfeng , Hu Xiaotong , Shao Ziqi , Xia Xiaohan , Sun Xiaomei , Zhang Qiang , Jin Meilin
Published: November 2021

African swine fever virus (ASFV) is an important viral pathogen infecting pigs worldwide throughout the pig industry. CD2v (an outer-membrane glycosylated protein of ASFV)-unexpressed lower-virulence mutants have appeared in China and other countries in recent years. Using OIE-recommended quantitative PCR and ELISA methods, people can accurately judge whether pigs are infected with wild-type ASFV. However, the strategy has failed to distinguish ΔCD2v lower-virulence mutants and wild-type ASFV infection. Here, we expressed and purified the CD2v and p30 proteins via CHO cells and successfully established a dual enzyme-linked immunosorbent assay (ELISA), which can be used to differentiate pigs infected with wild-type ASFV or with CD2v-unexpressed lower-virulence mutants. The dual ELISA showed excellent specificity without cross-reactions with antibodies of PRRSV, CSFV, JEV, PRV, or PPV. The dual ELISA could detect ASFV-infected positive serum samples up to dilutions of 5120 times, possessing high sensitivity. Therefore, the application of this dual ELISA approach can play an important role in ASFV epidemiology study and fill the gaps in differential diagnosis.
Protective or Risk Factors for Postoperative Pancreatic Fistulas in Malignant Pathology
Authors: Vasilescu Alin , Andriesi Rusu Delia , Bradea Costel , Vlad Nutu , Lupascu-Ursulescu Corina , Spiridon Irene , Trofin Ana , Tarcoveanu Eugen , Lupascu Cristian
Published: November 2021

Introduction: Malignant tumors are associated with a low incidence of postoperative pancreatic fistulas. The presence of peritumoral fibrosis is considered the protective factor for the development of postoperative pancreatic fistulas after pancreatic resections for pancreatic ductal adenocarcinomas. Methods: We analyzed a series of 109 consecutive patients with pancreatic resections for malignant pathology: pancreatic ductal adenocarcinomas and periampullary adenocarcinomas. The incidence of postoperative pancreatic fistulas has been reported in tumor histological type, in the presence of peritumoral fibrosis, and in the association between adenocarcinomas and areas of acute pancreatitis. The data obtained were processed with the statistical analysis program SPSS, and statistically significant p were considered at a value <0.05. Results: For the entire study group, the incidence of postoperative pancreatic fistulas was 11.01%. The lowest incidence was observed in the group of patients with pancreatic ductal adenocarcinomas (4.06% vs. 25.72% in the group with periampullary adenocarcinoma), with a p = 0.002. The presence of peritumoral fibrous tissue was observed in 49.31% of cases without pancreatic fistulas, and in 54.54% of cases that developed this postoperative complication (p = 0.5). Also, the peritumoral fibrous tissue had a uniform distribution depending on the main diagnosis (56.14% in pancreatic ductal adenocarcinoma group vs. 37.04% in periampullary adenocarcinoma group, with a p = 0.08). In the group of patients who associated areas of acute pancreatitis on the resections, the incidence of postoperative pancreatic fistulas was 7.8 times higher (30% vs. 3.8%, p = 0.026). Conclusions: Peritumoral fibrous tissue was not a factor involved in the developing of postoperative pancreatic fistulas. The association of adenocarciomas with areas of acute pancreatitis has led to a significant increase in postoperative pancreatic fistulas, which is a significant and independent risk factor.
Co-Evolution of Opioid and Adrenergic Ligands and Receptors: Shared, Complementary Modules Explain Evolution of Functional Interactions and Suggest Novel Engineering Possibilities
Authors: Root-Bernstein Robert , Churchill Beth
Published: November 2021

Cross-talk between opioid and adrenergic receptors is well-characterized and involves second messenger systems, the formation of receptor heterodimers, and the presence of extracellular allosteric binding regions for the complementary ligand; however, the evolutionary origins of these interactions have not been investigated. We propose that opioid and adrenergic ligands and receptors co-evolved from a common set of modular precursors so that they share binding functions. We demonstrate the plausibility of this hypothesis through a review of experimental evidence for molecularly complementary modules and report unexpected homologies between the two receptor types. Briefly, opioids form homodimers also bind adrenergic compounds; opioids bind to conserved extracellular regions of adrenergic receptors while adrenergic compounds bind to conserved extracellular regions of opioid receptors; opioid-like modules appear in both sets of receptors within key ligand-binding regions. Transmembrane regions associated with homodimerization of each class of receptors are also highly conserved across receptor types and implicated in heterodimerization. This conservation of multiple functional modules suggests opioid–adrenergic ligand and receptor co-evolution and provides mechanisms for explaining the evolution of their crosstalk. These modules also suggest the structure of a primordial receptor, providing clues for engineering receptor functions.
2,7(3,6)-Diaryl(arylamino)-substituted Carbazoles as Components of OLEDs: A Review of the Last Decade
Authors: Krucaite Gintare , Grigalevicius Saulius
Published: November 2021

Organic light emitting diode (OLED) is a new, promising technology in the field of lighting and display applications due to the advantages offered by its organic electroactive derivatives over inorganic materials. OLEDs have prompted a great deal of investigations within academia as well as in industry because of their potential applications. The electroactive layers of OLEDs can be fabricated from low molecular weight derivatives by vapor deposition or from polymers by spin coating from their solution. Among the low-molar-mass compounds under investigation in this field, carbazole-based materials have been studied at length for their useful chemical and electronic characteristics. The carbazole is an electron-rich heterocyclic compound, whose structure can be easily modified by rather simple reactions in order to obtain 2,7(3,6)-diaryl(arylamino)-substituted carbazoles. The substituted derivatives are widely used for the formation of OLEDs due to their good charge carrier injection and transfer characteristics, electroluminescence, thermally activated delayed fluorescence, improved thermal and morphological stability as well as their thin film forming characteristics. On the other hand, relatively high triplet energies of some substituted carbazole-based compounds make them useful components as host materials even for wide bandgap triplet emitters. The present review focuses on 2,7(3,6)-diaryl(arylamino)-substituted carbazoles, which were described in the last decade and were applied as charge-transporting layers, fluorescent and phosphorescent emitters as well as host materials for OLED devices.
Enhanced Long-Term Reliability of Seal DeltaSpot Welded Dissimilar Joint between 6061 Aluminum Alloy and Galvannealed Steel via Excimer Laser Irradiation
Authors: Joo Sung-Min , Kim Young-Gon , Kwak Young-Jin , Yoo Dong Jin , Jeong Chang-U , Park Jeshin , Oh Min-Suk
Published: November 2021

Structural-adhesive-assisted DeltaSpot welding was used to improve the weldability and mechanical properties of dissimilar joints between 6061 aluminum alloy and galvannealed HSLA steel. Evaluation of the spot-weld-bonded surfaces from lap shear tests after long-term exposure to chloride and a humid atmosphere (5% NaCl, 35 °C) indicated that the long-term mechanical reliability of the dissimilar weld in a corrosive environment depends strongly on the adhesive–Al6061 alloy bond strength. Corrosive electrolyte infiltrated the epoxy-based adhesive/Al alloy interface, disrupting the chemical interactions and decreasing the adhesion via anodic undercutting of the Al alloy. Due to localized electrochemical galvanic reactions, the surrounding nugget matrix suffered accelerated anodic dissolution, resulting in an Al6061-T6 alloy plate with degraded adhesive strength and mechanical properties. KrF excimer laser irradiation of the Al alloy before adhesive bonding removed the weakly bonded native oxidic overlayers and altered the substrate topography. This afforded a low electrolyte permeability and prevented adhesive delamination, thereby enhancing the long-term stability of the chemical interactions between the adhesive and Al alloy substrate. The results demonstrate the application of excimer laser irradiation as a simple and environmentally friendly processing technology for robust adhesion and reliable bonding between 6061 aluminum alloy and galvannealed steel.
Mesoporous Networks of N-Vinylpyrrolidone with (di)Methacrylates as Precursors of Ecological Molecular Imprinted Polymers
Authors: Kurmaz Svetlana V. , Fadeeva Natalia V. , Gorshkova Anna I. , Kurochkin Sergey A. , Knerelman Eugenia I. , Davydova Galina I. , Torbov Vladimir I. , Dremova Nadezhda N. , Konev Dmitry V. , Kurmaz Vladimir A. , Ignatiev Vladislav M. , Emelyanova Nina S.
Published: November 2021

Mesoporous polymer networks were prepared via the cross-linking radical copolymerization of non-toxic hydrophilic N-vinylpyrrolidone (VP) with triethylene glycol dimethacrylate (TEGDM) and poly(ethylene glycol) methyl ester methacrylate (PEGMMA) in bulk, using appropriate soluble and thermodynamically compatible macromolecular additives with a branched structure as porogens. The branched copolymers of various monomer compositions were obtained by radical copolymerization in toluene, controlled by 1-decanethiol, and these materials were characterized by a wide set of physical chemical methods. The specific surface areas and surface morphology of the polymer networks were determined by nitrogen low-temperature adsorption or Rose Bengal (RB) sorption, depending on the copolymer compositions and scanning electron microscopy. The electrochemical properties of RB before and after its encapsulation into a branched VP copolymer were studied on a glassy carbon electrode and the interaction between these substances was observed. Quantum chemical modeling of RB-VP or RB-copolymer complexes has been carried out and sufficiently strong hydrogen bonds were found in these systems. The experimental and modeling data demonstrate the high potency of such mesoporous polymer networks as precursors of molecularly imprinted polymers for the recognition of fluorescent dyes as nanomarkers for biomedical practice.
Wear and Corrosion Resistance of AlSi10Mg–CP–Ti Metal–Metal Composite Materials Produced by Electro-Sinter-Forging
Authors: Gobber Federico Simone , Fracchia Elisa , Spotorno Roberto , Fais Alessandro , Manfredi Diego
Published: November 2021

Metal–metal composites are a class of composite materials studied for their high ductility and strength, but their potential applications are currently limited by the complex manufacturing processes involved. Electro-sinter-forging (ESF) is a single-pulse electro discharge sintering technique that proved its effectiveness in the rapid sintering of several metals, alloys, and composites. Previous studies proved the processability of Ti and AlSi10Mg by ESF to produce metal–metal composites and defined a correlation between microstructure and processing parameters. This paper presents the wear and corrosion characterizations of two metal–metal composites obtained via ESF with the following compositions: 20% Ti/80% AlSi10Mg and 20% AlSi10Mg/80% Ti. The two materials showed complementary resistance to wear and corrosion. A higher fraction of AlSi10Mg is responsible for forming a protective tribolayer in dry-sliding conditions, while a higher fraction of Titanium confers improved corrosion resistance due to its higher corrosion potential.
Surface Phenomena at the Interface between Silicon Carbide and Iron Alloy
Authors: Cholewa Mirosław , Wróbel Tomasz , Baron Czesław , Morys Marcin
Published: November 2021

The paper discusses a potential composite produced using the casting method, where the matrix is gray cast iron with flake graphite. The reinforcement is provided by granular carborundum (β-SiC). The article presents model studies aimed at identifying the phenomena at the contact boundary resulting from the interaction of the liquid matrix with solid reinforcement particles. The scope of the research included, primarily, the metallographic analysis of the microstructure of the resulting composite, carried out by using light (LOM) and scanning electron (SEM) microscopy with energy dispersive X-ray spectroscopy (EDS) analysis. The occurrence of metallic phases in the boundary zone was indicated, the contents and morphology of which can be optimized in order to achieve favorable functional properties, mainly the tribological properties of the composite. In addition, the results obtained confirm the possibility of producing similar composites based on selected iron alloys.
Special Issue “Advanced Materials for Gas Sensors”
Authors: Simion Cristian E.
Published: November 2021

Today’s view on gas sensors end-users is more directed toward miniaturization, low power consumption, and intelligent device integration aiming to reply to several hot issues such as high sensitivity, optimum selectivity, fast response/recovery transients, and good long time stability [...]
Numerical and Experimental Investigation on the Surface Defect Generation during the Hot Extrusion of Al6063 Alloy
Authors: Park Namsu , Song Yeonghwan , Jung Seon-Ho , Song Junghan , Lee Jongsup , Lee Heejong , Sung Hyun-Min , Bae Gihyun
Published: November 2021

The surface quality control of extruded products is a critical concern in the home appliance manufacturing industry owing to the increasing need for products with a high surface quality, in addition to the essential mechanical properties of the final product. The underlying issue with achieving high-quality extrusion products is that surface defects, especially those resulting in surface gloss differences, called white line defects, are only observed after surface treatment. In this study, we aim to investigate the cause of white line defect generation on the surface of an extruded product. Accordingly, an experimental extrusion program is established using an L-shaped die that has a noticeable change in its bearing length along the inner corner of its cross-sectional profile. Laboratory-scale experiments were performed for the L-shaped extrusion of homogenized Al 6063 alloy at various ram speeds, in order to induce surface defects, considering the production yield rate required for mass production. Subsequently, the microstructural changes near the surface failure region were investigated using an arbitrary Lagrangian–Eulerian (ALE) technique-based thermomechanical finite element (FE) analysis. To scale-up the defect observation method from laboratory-scale to production-scale manufacturing and confirm the reproducibility of the surface defect, scaled-up L-shaped extrusions were performed in an actual industrial production line. Finally, the potential cause of white line defect generation is discussed by comparing the numerical and metallurgical analyses, including the scanning electron microscopy (SEM) and electron backscatter diffraction (EBSD) observations.
Tribology and Anti-Ablation Properties of SiC-VN-MoS2/Ta Composite Coatings on Carbon/Carbon Composites from 25 to 800 °C
Authors: Cao Jun , Chen Jianbin , Wang Xinbo , Wen Jingbo
Published: November 2021

To improve the self-lubrication and anti-ablation performances of C/C (carbon/carbon) composites from 25 to 800 °C, we engineered three layers of composite coatings consisting of SiC–VN–MoS2/Ta to deposit on the surface of the C/C composites. The tribology and anti-ablation properties of the composite coatings were experimented under dry sliding wear. The equivalent stress and deformation of the composite coatings are studied. The results show that the CoFs (coefficients of friction) of the C/C composites are decreased by 156% at 800 °C due to the new generated self-lubricating compounds from the MoS2/Ta and VN coating. The anti-ablation of the C/C composites are improved by 25,300% due to the silicon glass, and the generated compounds from V, Mo and Si. The deformation of the C/C substrate under the protection of these coatings looks like a quadrangular star. The cack of the C/C composites is easily generated without the protection from coatings.
Electrodischarge Methods of Shaping the Cutting Ability of Superhard Grinding Wheels
Authors: Gołąbczak Marcin , Święcik Robert , Gołąbczak Andrzej , Kaczmarek Dariusz , Dębkowski Ryszard , Tomczyk Barbara
Published: November 2021

In the paper, the influence of the electrodischarge dressing methods of superhard grinding wheels on shaping their cutting ability are presented. The results of research concerning the influence of dressing conditions using a stationary electrode, rotating electrode and segmental tool electrode on shaping the cutting ability of the superhard grinding wheels are reported. The cutting ability of superhard grinding wheels is assessed using an external tester made of titanium alloy Ti-6Al-4V (with a thermocouple) to determine the grinding temperature and the relative volumetric grinding efficiency of the tool. The results of the research reveal the diversified usefulness of the analyzed methods. At the end of the article application conclusions concerning the adaptation of developed methods of electrodischarge dressing in the industry are formulated.
Evaluation of the Effects of Surface Treatment Methods on the Properties of Coral Aggregate and Concrete
Authors: Liu Jinming , Ju Boyu , Xie Wei , Zhou Ting , Xiao Haiying , Dong Shanliang , Yang Wenshu
Published: November 2021

Coral concrete has low cost and convenient materials, making it an excellent raw material for processing. However, its lower strength limits the application of coral concrete. Surface modification is expected to increase the properties of porous coral concrete. In this study, single and compound modification treatments were applied to the surface of a coral aggregate to improve its properties for promoting the mechanical performance of coral concrete. The results showed that the micro-aggregate effect and pozzolanic activity of granulated blast furnace slag (GBFS) and the permeability and polycondensation of sodium silicate (SS) could be mutually promoted. The GBFS and SS could effectively fill the pores of the coral aggregate, enhancing the properties of the aggregate, such as density and load-bearing capacity, and reducing the water absorption and crushing index by more than 50%. GBFS and SS could intensify and accelerate the hydration of cement, and generate a large number of hard hydration products at the interfacial transition zone (ITZ), which could strengthen the bonding between the aggregate and mortar, improving the strength of the ITZ. The compressive strength of the coral concrete was significantly increased.
Clustering Vertex-Weighted Graphs by Spectral Methods
Authors: García-Zapata Juan-Luis , Grácio Clara
Published: November 2021

Spectral techniques are often used to partition the set of vertices of a graph, or to form clusters. They are based on the Laplacian matrix. These techniques allow easily to integrate weights on the edges. In this work, we introduce a p-Laplacian, or a generalized Laplacian matrix with potential, which also allows us to take into account weights on the vertices. These vertex weights are independent of the edge weights. In this way, we can cluster with the importance of vertices, assigning more weight to some vertices than to others, not considering only the number of vertices. We also provide some bounds, similar to those of Chegeer, for the value of the minimal cut cost with weights at the vertices, as a function of the first non-zero eigenvalue of the p-Laplacian (an analog of the Fiedler eigenvalue).
Pedal Curves of the Mixed-Type Curves in the Lorentz-Minkowski Plane
Authors: Zhao Xin , Pei Donghe
Published: November 2021

In this paper, we consider the pedal curves of the mixed-type curves in the Lorentz–Minkowski plane R12. The pedal curve is always given by the pseudo-orthogonal projection of a fixed point on the tangent lines of the base curve. For a mixed-type curve, the pedal curve at lightlike points cannot always be defined. Herein, we investigate when the pedal curves of a mixed-type curve can be defined and define the pedal curves of the mixed-type curve using the lightcone frame. Then, we consider when the pedal curves of the mixed-type curve have singular points. We also investigate the relationship of the type of the points on the pedal curves and the type of the points on the base curve.
The Key Role of Ambulatory Blood Pressure Monitoring in the Detection of Masked Hypertension and Other Phenomena in Frail Geriatric Patients
Authors: Koudelka Marek , Sovová Eliška
Published: November 2021

Background and Objectives: This study aims to determine prevalence of masked uncontrolled hypertension (MUH) in frail geriatric patients with arterial hypertension and thus show the role of ambulatory blood pressure monitoring (ABPM) since hypertension occurs in more than 80% of people 60+ years and cardiovascular diseases are the main cause of death worldwide. Despite modern pharmacotherapy, use of combination therapy and normal office blood pressure (BP), patients’ prognoses might worsen due to inadequate therapy (never-detected MUH). Materials and Methods: 118 frail geriatric patients (84.2 ± 4.4 years) treated for arterial hypertension with office BP < 140/90 mmHg participated in the study. 24-h ABPM and clinical examination were performed. Results: Although patients were normotensive in the office, 24-h measurements showed that BP values in 72% of hypertensives were not in the target range: MUH was identified in 47 (40%) patients during 24 h, in 48 (41%) patients during daytime and nocturnal hypertension in 60 (51%) patients. Conclusions: ABPM is essential for frail geriatric patients due to high prevalence of MUH, which cannot be detected based on office BP measurements. ABPM also helps to detect exaggerated morning surge, isolated systolic hypertension, dipping/non-dipping, and set and properly manage adequate treatment, which reduces incidence of cardiovascular events and contributes to decreasing the financial burden of society.
Prognostic Value of Telomeric Zinc Finger-Associated Protein Expression in Adenocarcinoma and Squamous Cell Carcinoma of Lung
Authors: Kim Gun-Jik , Lee Jae-Ho , Chae Mincheol , Lee Deok-Heon
Published: November 2021

Background and Objectives: Telomeric zinc finger-associated protein (TZAP) is a telomere regulation protein, previously known as ZBTB48. It binds preferentially to elongated telomeres, competing with telomeric repeat factors 1 and 2. TZAP expression may be associated with carcinogenesis, however; this study has not yet been performed in lung cancer. In this study, we examined the clinicopathological and prognostic values of TZAP expression in non-small cell lung cancer (NSCLC). Materials and Methods: Data were collected from The Cancer Genome Atlas. The clinical and prognostic values of TZAP for NSCLC were examined in adenocarcinoma (AD) and squamous cell carcinoma (SCC). Results: TZAP expression significantly increased in NSCLC tissues compared with normal tissues. In AD, TZAP expression was lower in patients with higher T stage (p = 0.005), and was associated with lymph node stage in SCC (p = 0.005). Survival analysis showed shorter disease-free survival in AD patients with lower TZAP expression (p = 0.047). TZAP expression did not have other clinical or prognostic value for AD and SCC. Conclusions: TZAP expression is a potential prognostic marker for NSCLC, especially in patients with AD.
Natural Products Induce Lysosomal Membrane Permeabilization as an Anticancer Strategy
Authors: Halaby Reginald
Published: November 2021

Cancer is a global health and economic issue. The majority of anticancer therapies become ineffective due to frequent genomic turnover and chemoresistance. Furthermore, chemotherapy and radiation are non-specific, killing all rapidly dividing cells including healthy cells. In this review, we examine the ability of some natural products to induce lysosomal-mediated cell death in neoplastic cells as a way to kill them more specifically than conventional therapies. This list is by no means exhaustive. We postulate mechanisms to explain lysosomal membrane permeabilization and its role in triggering cell death in cancer cells.
Effect of Rotating Magnetic Field on Microstructure in AlCuSi Alloys
Authors: Mikolajczak Piotr
Published: November 2021

The solidification of AlCuSi alloys with Mn and Fe was studied by rotating a magnetic field to understand the effect of melt flow. The specimens solidified with a forced convection, low cooling rate and low temperature gradient. Electromagnetic stirring generated by an electric coil around the specimens caused a transformation from equiaxed dendritic to rosette morphology, occasionally with spheroids and minor dendrites. The transformation was quantitatively observed with a specific surface Sv, that decreased for almost all alloys and marked the flow effect on α-Al. The computer coupling of phase diagrams and thermochemistry (CALPHAD) technique was applied for the calculation of phase diagrams and property diagrams. Forced convection decreased secondary dendrite arm spacing λ2 in almost all alloys, while it increased slightly in one studied alloy. The length of detrimental β-Al5FeSi phases decreased in the alloy, where β starts to precipitate in the presence of α-Al, while increasing in alloys where β starts as first and grows in the fully liquid melt. The average overall dimension of the Mn-rich phases increased in almost all alloys, and the number density decreased under flow. The modification of spacing for AlSi-eutectics and Al2Cu was analyzed. It was found that the occurrence of Al2Cu does not influence the fluid flow and vice versa.
Implementation of the Chicot–Lesage Composite Hardness Model in a Determination of Absolute Hardness of Copper Coatings Obtained by the Electrodeposition Processes
Authors: Mladenović Ivana O. , Lamovec Jelena S. , Vasiljević-Radović Dana G. , Vasilić Rastko , Radojević Vesna J. , Nikolić Nebojša D.
Published: November 2021

The influence of various electrolysis parameters, such as the type of cathode, composition of the electrolyte and electrolysis time, on the morphology, structure and hardness of copper coatings has been investigated. Morphology and structure of the coatings were analyzed by scanning electron microscope (SEM), atomic force microscope (AFM) and X-ray diffraction (XRD), while coating hardness was examined by Vickers microindentation test applying the Chicot–Lesage (C–L) composite hardness model. Depending on the conditions of electrolysis, two types of Cu coatings were obtained: fine-grained mat coatings with a strong (220) preferred orientation from the sulfate electrolyte and smooth mirror bright coatings with a strong (200) preferred orientation from the electrolyte with added leveling/brightening additives. The mat coatings showed larger both measured composite and calculated coating hardness than the mirror bright coatings, that can be explained by the phenomena on boundary among grains. Independent of electrolysis conditions, the critical relative indentation depth (RID) of 0.14 was established for all types of the Cu coatings, separating the zone in which the composite hardness can be equaled with the coating hardness and the zone requiring an application of the C–L model for a determination of the absolute hardness of the Cu coatings.
Integrative Analysis of Metabolome and Microbiome in Patients with Progressive Alcohol-Associated Liver Disease
Authors: Gao Bei , Zhu Yixin , Gao Nan , Shen Weishou , Stärkel Peter , Schnabl Bernd
Published: November 2021

Alcohol-associated liver disease is one of the most prevalent diseases around the world, with 10–20% of patients developing progressive liver disease. To identify the complex and correlated nature of metabolic and microbial data types in progressive liver disease, we performed an integrated analysis of the fecal and serum metabolomes with the gut microbiome in a cohort of 38 subjects, including 15 patients with progressive liver disease, 16 patients with non-progressive liver disease, and 7 control subjects. We found that although patients were generally clustered in three groups according to disease status, metabolites showed better separation than microbial species. Furthermore, eight serum metabolites were correlated with two microbial species, among which seven metabolites were decreased in patients with progressive liver disease. Five fecal metabolites were correlated with three microbial species, among which four metabolites were decreased in patients with progressive liver disease. When predicting progressive liver disease from non-progressive liver disease using correlated metabolic and microbial signatures with the random forest model, correlated serum metabolites and microbial species showed great predictive power, with the area under the receiver operating characteristic curve achieving 0.91. The multi-omics signatures identified in this study are helpful for the early identification of patients with progressive alcohol-associated liver disease, which is a key step for therapeutic intervention.
Opposing Immune-Metabolic Signature in Visceral Versus Subcutaneous Adipose Tissue in Patients with Adenocarcinoma of the Oesophagus and the Oesophagogastric Junction
Authors: Heeran Aisling B. , McCready Jessica , Dunne Margaret R. , Donlon Noel E. , Nugent Timothy S. , Bhardwaj Anshul , Mitchelson Kathleen A. J. , Buckley Amy M. , Ravi Narayanasamy , Roche Helen M. , Reynolds John V. , Lynam-Lennon Niamh , O’Sullivan Jacintha
Published: November 2021

Oesophageal adenocarcinoma (OAC) is an exemplar model of obesity-associated cancer. Previous work in our group has demonstrated that overweight/obese OAC patients have better responses to neoadjuvant therapy, but the underlying mechanisms are unknown. Unravelling the immune–metabolic signatures of adipose tissue may provide insight for this observation. We hypothesised that different metabolic pathways predominate in visceral (VAT) and subcutaneous adipose tissue (SAT) and inflammatory secretions will differ between the fat depots. Real-time ex vivo metabolic profiles of VAT and SAT from 12 OAC patients were analysed. These samples were screened for the secretion of 54 inflammatory mediators, and data were correlated with patient body composition. Oxidative phosphorylation (OXPHOS) was significantly higher in VAT when compared to SAT. OXPHOS was significantly higher in the SAT of patients receiving neoadjuvant treatment. VEGF-A, VEGF-C, P1GF, Flt-1, bFGF, IL-15, IL-16, IL-17A, CRP, SAA, ICAM-1, VCAM-1, IL-2, IL-13, IFN-γ, and MIP-1β secretions were significantly higher from VAT than SAT. Higher levels of bFGF, Eotaxin-3, and TNF-α were secreted from the VAT of obese patients, while higher levels of IL-23 and TARC were secreted from the SAT of obese patients. The angiogenic factors, bFGF and VEGF-C, correlated with visceral fat area. Levels of OXPHOS are higher in VAT than SAT. Angiogenic, vascular injury and inflammatory cytokines are elevated in VAT versus SAT, indicating that VAT may promote inflammation, linked to regulating treatment response.
Enzyme Method-Based Microfluidic Chip for the Rapid Detection of Copper Ions
Authors: Yin Binfeng , Wan Xinhua , Qian Changcheng , Sohan A. S. M. Muhtasim Fuad , Zhou Teng , Yue Wenkai
Published: November 2021

Metal ions in high concentrations can pollute the marine environment. Human activities and industrial pollution are the causes of Cu2+ contamination. Here, we report our discovery of an enzyme method-based microfluidic that can be used to rapidly detect Cu2+ in seawater. In this method, Cu2+ is reduced to Cu+ to inhibit horseradish peroxidase (HRP) activity, which then results in the color distortion of the reaction solution. The chip provides both naked eye and spectrophotometer modalities. Cu2+ concentrations have an ideal linear relationship, with absorbance values ranging from 3.91 nM to 256 μM. The proposed enzyme method-based microfluidic chip detects Cu2+ with a limit of detection (LOD) of 0.87 nM. Other common metal ions do not affect the operation of the chip. The successful detection of Cu2+ was achieved using three real seawater samples, verifying the ability of the chip in practical applications. Furthermore, the chip realizes the functions of two AND gates in series and has potential practical implementations in biochemical detection and biological computing.
Extremophilic Microorganisms in Central Europe
Authors: Zgonik Vera , Mulec Janez , Eleršek Tina , Ogrinc Nives , Jamnik Polona , Ulrih Nataša Poklar
Published: November 2021

Extremophiles inhabit a wide variety of environments. Here we focus on extremophiles in moderate climates in central Europe, and particularly in Slovenia. Although multiple types of stress often occur in the same habitat, extremophiles are generally combined into groups according to the main stressor to which they are adapted. Several types of extremophiles, e.g., oligotrophs, are well represented and diverse in subsurface environments and karst regions. Psychrophiles thrive in ice caves and depressions with eternal snow and ice, with several globally distributed snow algae and psychrophilic bacteria that have been discovered in alpine glaciers. However, this area requires further research. Halophiles thrive in salterns while thermophiles inhabit thermal springs, although there is little data on such microorganisms in central Europe, despite many taxa being found globally. This review also includes the potential use of extremophiles in biotechnology and bioremediation applications.
Thin-Film Solar Cells Based on Selenized CuSbS2 Absorber
Authors: Zhao Minghao , Yu Junsheng , Fu Lijuan , Guan Youwei , Tang Hua , Li Lu , Cheng Jiang
Published: November 2021

Copper antimony sulfide (CuSbS2) has attracted significant interest as an earth-abundant photovoltaic absorber. However, the efficiency of the current CuSbS2 photovoltaic device is too low to meet the requirement of a large-scale application. In this study, selenylation was introduced to optimize the band structure and improve the device performance. Selenized CuSbS2 [CuSbS2(Se)] films were realized using porous CuSbS2 films prepared by spray deposition with a post-treatment in Se vapor. The as-prepared CuSbS2(Se) films exhibited a compact structure. X-ray diffraction and elemental analysis confirmed the effective doping of Se into the lattice by substituting a part of S in CuSbS2. Elemental analysis revealed a gradient distribution for Se from the top surface to the deeper regions, and the substitution rate was very high (>39%). Dark J–V characteristics and AC impedance spectroscopy analysis showed that selenylation significantly reduced the carrier recombination center. As a result, the selenized CuSbS2 device exhibited a significant efficiency improvement from 0.12% to 0.90%, which is much higher than that of the simply annealed device (0.46%), indicating this technique is a promising approach to improve the performance of CuSbS2 solar cells.
Silver-Protein Nanocomposites as Antimicrobial Agents
Authors: Sitohy Mahmoud , Al-Mohammadi Abdul-Raouf , Osman Ali , Abdel-Shafi Seham , El-Gazzar Nashwa , Hamdi Sara , Ismail Sameh H. , Enan Gamal
Published: November 2021

The use of nanomaterials alone or in composites with proteins is a promising alternative to inhibit pathogenic bacteria. In this regard, this study used seed proteins from both fenugreek (Trigonella foenum-graecum L.) (FNP) and mung bean (Viga radiate) (MNP), with silver nanoparticles (Ag-NPs) and nanocomposites of either Ag-NPs plus FNP (Ag-FNP) or Ag-NPs plus MNP (Ag-MNP) as inhibitory agents against pathogenic bacteria. FNP and MNP were isolated from fenugreek seeds and mung bean seeds, respectively, and fractionated using Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE). Both FNP and MNP were immobilized with Ag-NPs to synthesize the nanocomposites Ag-FNP and Ag-MNP, respectively. The physicochemical characteristics of Ag-NPs and their composites with proteins were studied by X-ray Diffraction (XRD), dynamic light scattering (DLS), the zeta potential, Scanning and Transmission Electron Microscopy (SEM and TEM, respectively), Atomic Force Microscopy (AFM), and the Brunauer–Emmett–Teller isotherm (BET), elucidating their structural parameters, size distribution, size charges, size surface morphology, particle shape, dimensional forms of particles, and specific surface area, respectively. The sole proteins, Ag-NPs, and their nanocomposites inhibited pathogenic Gram-positive and Gram-negative bacteria. The inhibitory activities of both nanocomposites (Ag-FNP and Ag-MNP) were more than those obtained by either Ag-NPs or proteins (FNP, MNP). Minimum inhibitory concentrations (MICs) of Ag-FNP were very low (20 and 10 µg mL−1) against Salmonellatyphimurium and Pseudomonasaerugenosa, respectively, but higher (162 µg mL−1) against E. coli and Listeriamonocytogenes. MICs of Ag-MNP were also very low (20 µg mL−1) against Staphylococcusaureus but higher (325 µg mL−1) against Listeriamonocytogenes. TEM images of Staphylococcusaureus and Salmonellatyphimurium, treated with Ag-FNP and Ag-MNP, at their MIC values, showed asymmetric, wrinkled exterior surfaces, cell deformations, cell depressions, and diminished cell numbers.
Flower-like SnO2 Nanoparticle Biofabrication Using Pometia pinnata Leaf Extract and Study on Its Photocatalytic and Antibacterial Activities
Authors: Fatimah Is , Purwiandono Gani , Hidayat Habibi , Sagadevan Suresh , Ghazali Sheikh Ahmad Izaddin Sheikh Mohd , Oh Won-Chun , Doong Ruey-An
Published: November 2021

The present study reported biofabrication of flower-like SnO2 nanoparticles using Pometia pinnata leaf extract. The study focused on the physicochemical characteristics of the prepared SnO2 nanoparticles and its activity as photocatalyst and antibacterial agent. The characterization was performed by XRD, SEM, TEM, UV-DRS and XPS analyses. Photocatalytic activity of the nanoparticles was examined on bromophenol blue photooxidation; meanwhile, the antibacterial activity was evaluated against Klebsiella pneumoniae, Escherichia coli Staphylococcus aureus and Streptococcus pyogenes. XRD and XPS analyses confirmed the single tetragonal SnO2 phase. The result from SEM analysis indicates the flower like morphology of SnO2 nanoparticles, and by TEM analysis, the nanoparticles were seen to be in uniform spherical shapes with a diameter ranging from 8 to 20 nm. SnO2 nanoparticles showed significant photocatalytic activity in photooxidation of bromophenol blue as the degradation efficiency reached 99.93%, and the photocatalyst exhibited the reusability as the degradation efficiency values were insignificantly changed until the fifth cycle. Antibacterial assay indicated that the synthesized SnO2 nanoparticles exhibit an inhibition of tested bacteria and showed a potential to be applied for further environmental and medical applications.