bluuebunny/crossref_metadata_2025 huggingface dataset:
COVID-19 and Economic Growth: Does Good Government Performance Pay Off?
Authors: König Michael , Winkler Adalbert
Published: July 2020

The coronavirus pandemic led to substantial revisions of 2020 GDP growth projections. We analyse whether and to what extent the quality of government policies in handling the health aspects of the crisis influence cross-country differences in the economic impact of the pandemic as projected by the OECD, the IMF and the World Bank. We measure policy quality by a recently published Economist Intelligence Unit index and a COVID-19 Misery index combining the stringency of government-imposed distancing measures with the COVID-19 fatality rate. Moreover, we control for international spillovers captured by trade openness and export exposure to tourism. Results for most specifications show that good government performance pays off as the respective countries record less severe revisions of growth forecasts. Only in a few cases, our findings suggest that the pandemic’s global effect might be so strong that actions by individual governments do not affect cross-country differences of growth revisions. Finally, there is broad evidence supporting the view that a country’s exposure to the global economy influences its growth outlook relative to other countries.
Detection of early cartilage degeneration in the tibiotalar joint using 3 T gagCEST imaging: a feasibility study
Authors: Abrar Daniel B. , Schleich Christoph , Radke Karl Ludger , Frenken Miriam , Stabinska Julia , Ljimani Alexandra , Wittsack Hans-Jörg , Antoch Gerald , Bittersohl Bernd , Hesper Tobias , Nebelung Sven , Müller-Lutz Anja
Published: July 2020

Objective

To establish and optimize a stable 3 Tesla (T) glycosaminoglycan chemical exchange saturation transfer (gagCEST) imaging protocol for assessing the articular cartilage of the tibiotalar joint in healthy volunteers and patients after a sustained injury to the ankle.

Methods

Using Bloch–McConnell simulations, we optimized the sequence protocol for a 3 T MRI scanner for maximum gagCEST effect size within a clinically feasible time frame of less than 07:30 min. This protocol was then used to analyze the gagCEST effect of the articular cartilage of the tibiotalar joint of 17 healthy volunteers and five patients with osteochondral lesions of the talus following ankle trauma. Reproducibility was tested with the intraclass correlation coefficient.

Results

The mean magnetization transfer ratio asymmetry (MTRasym), i.e., the gagCEST effect size, was significantly lower in patients than in healthy volunteers (0.34 ± 1.9% vs. 1.49 ± 0.11%; p < 0.001 [linear mixed model]). Intra- and inter-rater reproducibility was excellent with an average measure intraclass correlation coefficient (ICC) of 0.97 and a single measure ICC of 0.91 (p < 0.01).

Discussion

In this feasibility study, pre-morphological tibiotalar joint cartilage damage was quantitatively assessable on the basis of the optimized 3 T gagCEST imaging protocol that allowed stable quantification gagCEST effect sizes across a wide range of health and disease in clinically feasible acquisition times.
Effects of Shinrin-Yoku (Forest Bathing) and Nature Therapy on Mental Health: a Systematic Review and Meta-analysis
Authors: Kotera Yasuhiro , Richardson Miles , Sheffield David
Published: July 2020

Shinrin-yoku, immersing oneself in nature using one’s senses, has been receiving increased attention internationally. While most of the existing studies have focused on physical health, this systematic review and meta-analysis examined the mental health impacts of shinrin-yoku, using the PRISMA guidelines (PROSPERO registry: BLINDED). Of 497 articles retrieved on databases including PubMed/MEDLINE, PsycINFO, Science Direct and Google Scholar, twenty met the inclusion criteria. All studies were conducted in Asia and Europe, and used a variety of different bathing approaches (e.g. breathing, walking, yoga). While noting a need for more rigorous research and extensive follow-up assessments, the findings indicate that shinrin-yoku can be effective in reducing mental health symptoms in the short term, particularly anxiety. More careful examination of shinrin-yoku practices is needed; longer follow-up with participants from a range of countries along with greater examination of potential mechanisms is needed for shinrin-yoku to be accepted into mainstream interventions.
Umbilical Cord Mesenchymal Stem Cells in Amyotrophic Lateral Sclerosis: an Original Study
Authors: Barczewska Monika , Maksymowicz Stanisław , Zdolińska-Malinowska Izabela , Siwek Tomasz , Grudniak Mariusz
Published: July 2020

Objective

Amyotrophic lateral sclerosis (ALS) is still incurable. Although different therapies can affect the health and survival of patients. Our aim is to evaluate the effect of umbilical mesenchymal stem cells administrated intrathecally to patients with amyotrophic lateral sclerosis on disability development and survival.

Methods

This case-control study involved 67 patients treated with Wharton’s jelly mesenchymal stem cells (WJ-MSC). The treated patients were paired with 67 reference patients from the PRO-ACT database which contains patient records from 23 ALS clinical studies (phase 2/3). Patients in the treatment and reference groups were fully matched in terms of race, sex, onset of symptoms (bulbar/spinal), FT9 disease stage at the beginning of therapy and concomitant amyotrophic lateral sclerosis medications. Progression rates prior to treatment varied within a range of ± 0.5 points. All patients received three intrathecal injections of Wharton’s jelly-derived mesenchymal stem cells every two months at a dose of 30 × 106 cells. Patients were assessed using the ALSFRS-R scale. Survival times were followed-up until March 2020.

Results

Median survival time increased two-fold in all groups. In terms of progression, three response types measured in ALSFRS-R were observed: decreased progression rate (n = 21, 31.3%), no change in progression rate (n = 33, 49.3%) and increased progression rate (n = 13, 19.4%). Risk-benefit ratios were favorable in all groups. No serious adverse drug reactions were observed.

Interpretation

Wharton’s jelly-derived mesenchymal stem cells therapy is safe and effective in some ALS patients, regardless of the clinical features and demographic factors excluding sex. The female sex and a good therapeutic response to the first administration are significant predictors of efficacy following further administrations.
Wind Erosion Climate Change in Northern China During 1981–2016
Authors: Zhang Feng , Wang Jing’ai , Zou Xueyong , Mao Rui , Gong Daoyi , Feng Xingya
Published: July 2020

Wind erosion is largely controlled by climate conditions. In this study, we examined the influences of changes in wind speed, soil wetness, snow cover, and vegetation cover related to climate change on wind erosion in northern China during 1981–2016. We used the wind erosion force, defined as wind factor in the Revised Wind Erosion Equation Model, to describe the effect of wind speed on wind erosion. The results show that wind erosion force presented a long-term decreasing trend in the southern Northwest, northern Northwest, and eastern northern China during 1981–2016. In the Gobi Desert, the wind erosion force presented for 1981–1992 a decreasing trend, for 1992–2012 an increasing trend, and thereafter a weakly decreasing trend. In comparison to wind speed, soil wetness and snow cover had weaker influences on wind erosion in northern China, while vegetation cover played a significant role in the decrease of wind erosion in the eastern northern China during 1982–2015.
Revisiting Access to Cultural Heritage in the Public Domain: EU and International Developments
Authors: Wallace Andrea , Euler Ellen
Published: July 2020

In the past year, a number of legal developments have accelerated discussions around whether intellectual property rights can be claimed in materials generated during the reproduction of public domain works. This article analyses those developments, focusing on the 2018 German Federal Supreme Court decision Museumsfotos, Art. 14 of the 2019 Copyright and Related Rights in the Digital Single Market Directive, and relevant provisions of the 2019 Open Data and the Re-use of Public Sector Information Directive. It reveals that despite the growing consensus for protecting the public domain, there is a lack of practical guidance throughout the EU in legislation, jurisprudence, and literature on what reproduction media might attract new intellectual property rights, from scans to photography to 3D data. This leaves ample room for copyright to be claimed in reproduction materials produced by new technologies. Moreover, owners remain able to impose other restrictive measures around public domain works and data, like onsite photography bans, website terms and conditions, and exclusive arrangements with third parties. This article maps out these various legal gaps. It argues the pro-open culture spirit of the EU Directives should be embraced and provides guidance for Member States and heritage institutions around national implementation.
Depth-dependent artifacts resulting from ApRES signal clipping
Authors: Vaňková Irena , Nicholls Keith W. , Xie Surui , Parizek Byron R. , Voytenko Denis , Holland David M.
Published: July 2020

Several autonomous phase-sensitive radio-echo sounders (ApRES) were deployed at Greenland glaciers to investigate ice deformation. Different attenuation settings were tested and it was observed that, in the presence of clipping of the deramped ApRES signal, each setting produced a different result. Specifically, higher levels of clipping associated with lower attenuation produced an apparent linear increase of diurnal vertical cumulative displacement with depth, and obscured the visibility of the basal reflector in the return amplitude. An example with a synthetic deramped signal confirmed that these types of artifacts result from the introduction of harmonics from square-wave-like features introduced by clipping. Apparent linear increase of vertical displacement with depth occurs when the vertical position of a near-surface internal reflector changes in time. Artifacts in the return amplitude may obscure returns from internal reflectors and the basal reflector, making it difficult to detect thickness evolution of the ice and to correctly estimate vertical velocities. Variations in surface melt during ApRES deployments can substantially modulate the received signal strength on short timescales, and we therefore recommend using higher attenuator settings for deployments in such locations.
First record of a shore bug (Insecta, Hemiptera, Saldidae) from Gondwana
Authors: Moura-Júnior Dionizio A. , Scheffler Sandro M. , Moreira Felipe F. F. , Nel André , Mejdalani Gabriel
Published: July 2020

A new Saldidae (MCT 6959-I) from the Crato Formation (Lower Cretaceous, late Aptian), Santana Group, Araripe Basin (northeastern Brazil) is described and illustrated.Olindasalda gondwanican. gen. n. sp. is the first fossil Saldidae recorded from the Gondwanan supercontinent. The new genus can be distinguished from other members of the subfamily Chiloxanthinae by its small (4.49 mm) and oval-elongated body, absence of spots on the corium of the hemelytron, long R vein, and much-reduced first cell of the membrane. Remarks on the geology of the type locality and a comparison of the new genus with other saldids are provided.

UUID:http://zoobank.org/5ef8db72-3d7c-4de0-9a7c-e8fd80a9c8ba
REGIONAL DISPARITIES IN LABOUR PRODUCTIVITY AND THE ROLE OF CAPITAL STOCK
Authors: Gardiner Ben , Fingleton Bernard , Martin Ron
Published: July 2020

This paper reports on the availability of regional capital stock data,1 in the form of new/updated regional (NUTS2 level) capital stock estimates,2 building on an approach (Perpetual Inventory Method) which had been previously developed for the European Commission. The particular focus here is on the UK and how these data are used to shed light on regional labour productivity disparities. Using a NUTS2 level dataset constructed for the period 2000–16, we use a dynamic spatial panel approach from Baltagi et al. (2019) to estimate a model relating productivity to output (growth or levels) and augmented by explicit incorporation of capital stock plus various other covariates such as human capital. We find that regional variations in capital stocks per worker make a significant contribution to regional variations in labour productivity, but the geography of human capital is also highly relevant. Moreover, we give evidence to show that as human capital rises, notably as we move from the regions to London, the impact of capital stock per worker is less. The effect of capital stock depends on the level of human capital.
LIVING WITH COVID-19: BALANCING COSTS AGAINST BENEFITS IN THE FACE OF THE VIRUS
Authors: Miles David , Stedman Mike , Heald Adrian
Published: July 2020

This paper analyses the costs and benefits of lockdown policies in the face of COVID-19. What matters for people is the quality and length of lives and one should measure costs and benefits in terms of those things. That raises difficulties in measurement, particularly in valuing potential lives saved. We draw upon guidelines used in the UK for public health decisions, as well as other measures, which allow a comparison between health effects and other economic effects. We look at evidence on the effectiveness of past severe restrictions applied in European countries, focusing on the evidence from the UK. The paper considers policy options for the degree to which restrictions are eased. There is a need to normalise how we view COVID because its costs and risks are comparable to other health problems (such as cancer, heart problems, diabetes) where governments have made resource decisions for decades. The lockdown is a public health policy and we have valued its impact using the tools that guide health care decisions in the UK public health system. The evidence suggests that the costs of continuing severe restrictions in the UK are large relative to likely benefits so that a substantial easing in general restrictions in favour of more targeted measures is warranted.
COVID-19 IMPACTS ON DESTITUTION IN THE UK
Authors: Bhattacharjee Arnab , Lisauskaite Elena
Published: July 2020

We use microsimulation combined with a model of the COVID-19 impacts on individuals and households to obtain projections of households in destitution in the United Kingdom. The projections are estimated at two levels: aggregate quarterly for the UK, for all quarters of 2020; and annual for 2020 differentiated by region, sector and household demographics. At the aggregate level, destitution is projected to be about three times higher than the non-COVID counterfactual level in 2020Q2, as well as substantially higher than the non-COVID case for the remainder of the year. This increased destitution is initially largely due to the effect on the self-employed, and as the Furlough scheme is drawn down, also on the unemployed. Impacts upon different regions and sectors vary widely, and so do variations across different household types. The sectors particularly affected are construction and manufacturing, while London and its closely connected regions (South East and the Midlands) are most severely affected. Single adult households suffer the most, and the adverse effects increase with number of children in the household. That the effects upon youth remain high is a particularly worrying sign, and very high increases in destitution are also projected for 25–54 year olds and the elderly (75 years and older). Further, severe adverse effects are projected for sections of society and the economy where multiple impacts are coincident. Robust and sustained mitigation measures are therefore required.
Association of fibre degradation with ruminal dissolved hydrogen in growing beef bulls fed with two types of forages
Authors: Wang Rong , Wang Min , Lin Bo , Ma Zhi Yuan , Ungerfeld Emilio M. , Wu Ting Ting , Wen Jiang Nan , Zhang Xiu Min , Deng Jin Ping , Tan Zhi Liang
Published: July 2020

The present study investigated the association between fibre degradation and the concentration of dissolved molecular hydrogen (H2) in the rumen. Napier grass (NG) silage and corn stover (CS) silage were compared as forages with contrasting structures and degradation patterns. In the first experiment, CS silage had greater 48-h DM, neutral-detergent fibre (NDF) and acid-detergent fibre degradation, and total gas and methane (CH4) volumes, and lower 48-h H2 volume than NG silage in 48-h in vitro incubations. In the second experiment, twenty-four growing beef bulls were fed diets including 55 % (DM basis) NG or CS silages. Bulls fed the CS diet had greater DM intake (DMI), average daily gain, total-tract digestibility of OM and NDF, ruminal dissolved methane (dCH4) concentration and gene copies of protozoa, methanogens, Ruminococcus albus and R. flavefaciens, and had lower ruminal dH2 concentration, and molar proportions of valerate and isovalerate, in comparison with those fed the NG diet. There was a negative correlation between dH2 concentration and NDF digestibility in bulls fed the CS diet, and a lack of relationship between dH2 concentration and NDF digestibility with the NG diet. In summary, the fibre of CS silage was more easily degraded by rumen microorganisms than that of NG silage. Increased dCH4 concentration with the CS diet presumably led to the decreased ruminal dH2 concentration, which may be helpful for fibre degradation and growth of fibrolytic micro-organisms in the rumen.
Detection of gluten in duplicate portions to determine gluten intake of coeliac disease patients on a gluten-free diet
Authors: van der Fels-Klerx H. J. , Smits N. G. E. , Bremer M. G. E. G. , Schultink J. M. , Nijkamp M. M. , Castenmiller J. J. M. , de Vries J. H. M.
Published: July 2020

This study determined the gluten content of foods and meals consumed by coeliac disease (CD) patients who adhere to a gluten-free diet, and to estimate the total daily intake of gluten of these patients. CD patients fulfilling defined inclusion criteria were preselected and approached for participation in the study. Duplicate portions (DP) of foods and mixed dishes were collected from the CD patients for evaluating complete daily food intake during two individual days. Also, for these days, written food records were completed by the participants. From each DP, a laboratory sample was prepared and analysed for its gluten concentration and total daily gluten intake was calculated. Each individual’s total daily intakes of energy and macronutrients were calculated using the Dutch food composition database. In total, twenty-seven CD patients participated, seven males and twenty females, aged between 21 and 64 years. In thirty-two (6 %) of 499 food samples collected in total, more than 3 mg/kg gluten was present. In four of these thirty-two samples, the gluten concentration was above the European legal limit of 20 mg/kg and three of the four samples had a gluten-free label. The maximal gluten intake was 3·3 mg gluten/d. The gluten tolerance for sensitive CD patients (>0·75 mg/d) was exceeded on at least six out of fifty-four study days. To also protect these sensitive CD patients, legal thresholds should be re-evaluated and the detection limit of analytical methods for gluten analysis lowered.
Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (Ctenopharyngodon idella)
Authors: Fang Chuang-Chuang , Feng Lin , Jiang Wei-Dan , Wu Pei , Liu Yang , Kuang Sheng-Yao , Tang Ling , Liu Xiang-An , Zhou Xiao-Qiu
Published: July 2020

In the current research, a 60-d experiment was conducted with the purpose of exploring the impacts of methionine (Met) on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis as well as the related signalling pathway. Six diets (iso-nitrogenous) differing in Met concentrations (2·54, 4·85, 7·43, 10·12, 12·40 and 15·11 g/kg diets) were fed to 540 grass carp (178·47 (SD0·36) g). Results showed (P <0·05) that compared with Met deficiency, optimal level of dietary Met (1) increased feed intake, feed efficiency, specific growth rate and percentage weight gain (PWG); (2) increased fish muscle protein, lipid and free amino acid contents and improved fish muscle fatty acid profile as well as increased protein content in part associated with the target of rapamycin complex 1 (TORC1)/S6K1 signalling pathway; (3) increased the frequency distribution of muscle fibre with >50 µm of diameter; (4) increased type I collagen synthesis partly related to the transforming growth factor-β1/Smads and CK2/TORC1 signalling pathways. In conclusion, dietary Met improved muscle growth, which might be due to the regulation of muscle nutritive deposition, muscle fibre growth and type I collagen synthesis-related signal molecules. Finally, according to PWG and muscle collagen content, the Met requirements for on-growing grass carp (178–626 g) were estimated to be 9·56 g/kg diet (33·26 g/kg protein of diet) and 9·28 g/kg diet (32·29 g/kg of dietary protein), respectively.
DISSIDENCE, DICTATORSHIP, AND DEMOCRACY: THE STRUGGLES OF MALIAN EXILES IN AFRICA AND BEYOND, 1968–91
Authors: Dedieu Jean-Philippe
Published: July 2020

In contrast with the imperial period, historians have overlooked African exile politics during the subsequent decades of one-party and military rule. Focusing on the Malian case, this article proceeds in three parts. The first section explores the creation in Africa, in particular in Ivory Coast and Senegal, of clandestine opposition movements to Moussa Traoré's regime. The second section focuses on Europe, particularly France, where dissidents benefitted from an unparalleled openness of the political system compared to that seen in African countries. The final section investigates the influence of these networks spanning Africa and Europe on the formation of pro-democracy organizations in Mali and the final overthrow of the Traoré regime in 1991. Theorizing exile as a process which enabled activists to operate in abeyance despite repression – before being able to emerge more openly – refines our understanding of political transitions which were driven by the juncture of internal and external dynamics.
Field burn versus fire-related cues: germination from the soil seed bank of a South American temperate grassland
Authors: Cuello Noelia , López-Mársico Luis , Rodríguez Claudia
Published: July 2020

Fire and grazing are large-scale disturbances that shape the structure and function of open habitats. In temperate grasslands of southern South America, fire is used as a management tool to control tussock grasses and improve forage quality. In this study, we examined if fire and two of its components (heat and smoke) affect germination from the soil seed bank of a temperate grassland in Uruguay. Soil samples were extracted from a recently burned site and from an adjacent area that had not been burned for at least 4 years. The latter was subjected to four treatments: (1) heat shock, (2) smoke, (3) heat shock and smoke and (4) control. The samples were placed in a germination chamber and germination was recorded for 140 days. Field burn was the treatment that differed most from the control. This treatment produced a significant increase in density and richness of germinants and the germination peak preceded those of the remaining treatments. The three treatments involving fire-related cues did not affect the seedling richness and density, but the germination of some individual species was enhanced by some of them, mainly those in which the seeds were smoked. Our results show that fire and its components stimulate the germination of some species of the Río de la Plata grasslands, contrary to what had been observed previously in the region. We also suggest that, unlike Mediterranean-type systems, other fire cues, alone or in combination with heat and smoke, may promote germination after a fire event.
Social anxiety disorder in children: investigating the relative contribution of automatic thoughts, repetitive negative thinking and metacognitions
Authors: Esbjørn Barbara Hoff , Falch Anette , Walczak Monika Anna , Normann Nicoline , Breinholst Sonja
Published: July 2020

Background:

Social anxiety disorder (SAD) is common in youths. However, our understanding of SAD in children is inferior to that of SAD in adolescents or adults, and it is unclear if known adult SAD maintenance mechanisms may also operate in children with SAD.

Aim:

The paper sets out to investigate the specificity of positive automatic thoughts, social threat negative automatic thoughts, repetitive negative thinking, positive and negative metacognitions in predicting SAD symptoms and diagnoses in clinically anxious children.

Method:

We enrolled 122 clinically anxious children aged 7–13 years; of these, 33 had an SAD diagnosis.

Results:

SAD symptoms correlated positively with social threat negative automatic thoughts, repetitive negative thinking, and negative metacognitions, and negatively with positive automatic thoughts. Linear regression indicated that, of these variables, only social threat negative automatic thoughts predicted social anxiety symptoms. Logistic regression indicated that social threat negative automatic thoughts, a higher number of diagnoses and negative metacognitive beliefs specifically predicted the presence of SAD diagnosis.

Conclusions:

Our findings suggest that content-specific social threat negative automatic thoughts was the only variable that specifically distinguished both higher levels of social anxiety symptoms and diagnoses.
Development of the Response to Fearful Situations Scale
Authors: McDermott Katherine A. , Fitch Kristin , Dillon Kirsten H. , Mueller Nora E. , Carlton Corinne N. , Schmidt Norman B. , Cougle Jesse R.
Published: July 2020

Background:

Most measures of anxious avoidance are limited to disorder-specific mechanisms and ignore the measurement of courage/approach responding in confronting fearful situations.

Aims:

The purpose of the present study was to construct and validate a self-report assessment of the tendency towards avoidant or approach responding in fearful situations, the Response to Fearful Situations Scale (RFSS).

Method and Results:

In Study 1 (n = 241), exploratory factor analysis resulted in two factors, avoidance and approach. Study 2 (n = 423) replicated the two-factor structure and established test–re-test reliability. In Study 3 (n = 44), the RFSS demonstrated predictive validity on a behavioural avoidance task. In Studies 4 (n = 253) and 5 (n = 256), the RFSS was associated with clinical symptoms above existing measures of avoidance.

Discussion:

These results validate the use of the RFSS as a transdiagnostic measure of avoidance and approach.
Three Decades of Divergent Land Use and Plant Community Change Alters Soil C and N Content in Tallgrass Prairie
Authors: Connell R. Kent , Nippert Jesse B. , Blair John M.
Published: July 2020

Frequent fire and grazing by megafauna are important determinants of tallgrass prairie plant community structure. However, fire suppression and removal of native grazers have altered these natural disturbance regimes and changed grassland plant communities with potential long‐term consequences for soil carbon (C) and nitrogen (N) storage. We investigated multidecade changes in soil C and N pools in response to contrasting long‐term burning and grazing treatments. Fire suppression with or without grazers and exclusion of grazers in annually burned prairie increased soil C content and shifted the δ13C signature of soil C over time, concomitant with changes in plant community composition. Soil δ13C values indicated that increased soil C content was associated with an increased contribution from plants using a C3 photosynthetic pathway (i.e., woody shrubs) under fire suppression. Soil N content also increased when fire was suppressed, relative to frequently burned grassland, but the rate of increase was slower when grazers were present. Additionally, changes in δ15N values suggested that grazing increased the openness of the N cycle, presumably due to greater N losses. By coupling long‐term fire and grazing treatments with plant community data and soil samples archived over three decades, we demonstrate that human‐caused changes to natural disturbance regimes in a tallgrass prairie significantly alter soil C and N cycles through belowground changes associated with shifts in the plant community. Since natural disturbance regimes have been altered in grasslands across the world, our results are relevant for understanding the long‐term biogeochemical consequences of these ongoing land use changes.
Carbon Dioxide (CO2) Fluxes From Terrestrial and Aquatic Environments in a High‐Altitude Tropical Catchment
Authors: Schneider Chloe L. , Herrera Maribel , Raisle Megan L. , Murray Andrew R. , Whitmore Keridwen M. , Encalada Andrea C. , Suárez Esteban , Riveros‐Iregui Diego A.
Published: July 2020

High‐altitude tropical grasslands, known as “páramos,” are characterized by high solar radiation, high precipitation, and low temperature. They also exhibit some of the highest ecosystem carbon stocks per unit area on Earth. Recent observations have shown that páramos may be a net source of CO2 to the atmosphere as a result of climate change; however, little is known about the source of this excess CO2 in these mountainous environments or which landscape components contribute the most CO2. We evaluated the spatial and temporal variability of surface CO2 fluxes to the atmosphere from adjacent terrestrial and aquatic environments in a high‐altitude catchment of Ecuador, based on a suite of field measurements performed during the wet season. Our findings revealed the importance of hydrologic dynamics in regulating the magnitude and likely fate of dissolved carbon in the stream. While headwater catchments are known to contribute disproportionately larger amounts of carbon to the atmosphere than their downstream counterparts, our study highlights the spatial heterogeneity of CO2 fluxes within and between aquatic and terrestrial landscape elements in headwater catchments of complex topography. Our findings revealed that CO2 evasion from stream surfaces was up to an order of magnitude greater than soil CO2 efflux from the adjacent terrestrial environment. Stream carbon flux to the atmosphere appeared to be transport limited (i.e., controlled by flow characteristics, turbulent flow, and water velocity) in the upper reaches of the stream, and source limited (i.e., controlled by CO2 and carbon availability) in the lower reaches of the stream. A 4‐m waterfall along the channel accounted for up to 35% of the total evasion observed along a 250‐m stream reach. These findings represent a first step in understanding ecosystem carbon cycling at the interface of terrestrial and aquatic ecosystems in high‐altitude, tropical, headwater catchments.
Willingness to participate in genome testing: a survey of public attitudes from Qatar
Authors: Abdul Rahim Hanan F. , Ismail Said I. , Hassan Amel , Fadl Tasnim , Khaled Salma M. , Shockley Bethany , Nasrallah Catherine , Qutteina Yara , Elmaghraby Engi , Yasin Heba , Darwish Dima , Fakhro Khalid A. , Badji Radja , Al-Muftah Wadha , Afifi Nahla , Althani Asmaa
Published: July 2020

Genomics has the potential to revolutionize medical approaches to disease prevention, diagnosis, and treatment, but it does not come without challenges. The success of a national population-based genome program, like the Qatar Genome Program (QGP), depends on the willingness of citizens to donate samples and take up genomic testing services. This study explores public attitudes of the Qatari population toward genetic testing and toward participating in the QGP. A representative sample of 837 adult Qataris was surveyed in May 2016. Approximately 71% of respondents surveyed reported that they were willing to participate in the activities of the QGP. Willingness to participate was significantly associated with basic literacy in genetics, a family history of genetic diseases, and previous experience with genetic testing through premarital screening. Respondents cited the desire to know more about their health status as the principle motivation for participating, while lack of time and information were reported as the most important barriers. With QGP plans to ramp up the scale of its national operation toward more integration into clinical care settings, it is critical to understand public attitudes and their determinants. The results demonstrate public support but also identify the need for more education and individual counseling that not only provide information on the process, challenges, and benefits of genomic testing, but that also address concerns about information security.
Dissociable mesolimbic dopamine circuits control responding triggered by alcohol-predictive discrete cues and contexts
Authors: Valyear Milan D. , Glovaci Iulia , Zaari Audrey , Lahlou Soraya , Trujillo-Pisanty Ivan , Andrew Chapman C. , Chaudhri Nadia
Published: July 2020

Context can influence reactions to environmental cues and this elemental process has implications for substance use disorder. Using an animal model, we show that an alcohol-associated context elevates entry into a fluid port triggered by a conditioned stimulus (CS) that predicted alcohol (CS-triggered alcohol-seeking). This effect persists across multiple sessions and, after it diminishes in extinction, the alcohol context retains the capacity to augment reinstatement. Systemically administered eticlopride and chemogenetic inhibition of ventral tegmental area (VTA) dopamine neurons reduce CS-triggered alcohol-seeking. Chemogenetically silencing VTA dopamine terminals in the nucleus accumbens (NAc) core reduces CS-triggered alcohol-seeking, irrespective of context, whereas silencing VTA dopamine terminals in the NAc shell selectively reduces the elevation of CS-triggered alcohol-seeking in an alcohol context. This dissociation reveals new roles for divergent mesolimbic dopamine circuits in the control of responding to a discrete cue for alcohol and in the amplification of this behaviour in an alcohol context.
Assembly of a patchy protein into variable 2D lattices via tunable multiscale interactions
Authors: Zhang Shuai , Alberstein Robert G. , De Yoreo James J. , Tezcan F. Akif
Published: July 2020

Self-assembly of molecular building blocks into higher-order structures is exploited in living systems to create functional complexity and represents a powerful strategy for constructing new materials. As nanoscale building blocks, proteins offer unique advantages, including monodispersity and atomically tunable interactions. Yet, control of protein self-assembly has been limited compared to inorganic or polymeric nanoparticles, which lack such attributes. Here, we report modular self-assembly of an engineered protein into four physicochemically distinct, precisely patterned 2D crystals via control of four classes of interactions spanning Ångström to several-nanometer length scales. We relate the resulting structures to the underlying free-energy landscape by combining in-situ atomic force microscopy observations of assembly with thermodynamic analyses of protein-protein and -surface interactions. Our results demonstrate rich phase behavior obtainable from a single, highly patchy protein when interactions acting over multiple length scales are exploited and predict unusual bulk-scale properties for protein-based materials that ensue from such control.
An immune receptor complex evolved in soybean to perceive a polymorphic bacterial flagellin
Authors: Wei Yali , Balaceanu Alexandra , Rufian Jose S. , Segonzac Cécile , Zhao Achen , Morcillo Rafael J. L. , Macho Alberto P.
Published: July 2020

In both animals and plants, the perception of bacterial flagella by immune receptors elicits the activation of defence responses. Most plants are able to perceive the highly conserved epitope flg22 from flagellin, the main flagellar protein, from most bacterial species. However, flagellin from Ralstonia solanacearum, the causal agent of the bacterial wilt disease, presents a polymorphic flg22 sequence (flg22Rso) that avoids perception by all plants studied to date. In this work, we show that soybean has developed polymorphic versions of the flg22 receptors that are able to perceive flg22Rso. Furthermore, we identify key residues responsible for both the evasion of perception by flg22Rso in Arabidopsis and the gain of perception by the soybean receptors. Heterologous expression of the soybean flg22 receptors in susceptible plant species, such as tomato, enhances resistance to bacterial wilt disease, demonstrating the potential of these receptors to enhance disease resistance in crop plants.
Cryptic evolved melts beneath monotonous basaltic shield volcanoes in the Galápagos Archipelago
Authors: Stock Michael J. , Geist Dennis , Neave David A. , Gleeson Matthew L. M. , Bernard Benjamin , Howard Keith A. , Buisman Iris , Maclennan John
Published: July 2020

Many volcanoes erupt compositionally homogeneous magmas over timescales ranging from decades to millennia. This monotonous activity is thought to reflect a high degree of chemical homogeneity in their magmatic systems, leading to predictable eruptive behaviour. We combine petrological analyses of erupted crystals with new thermodynamic models to characterise the diversity of melts in magmatic systems beneath monotonous shield volcanoes in the Galápagos Archipelago (Wolf and Fernandina). In contrast with the uniform basaltic magmas erupted at the surface over long timescales, we find that the sub-volcanic systems contain extreme heterogeneity, with melts extending to rhyolitic compositions. Evolved melts are in low abundance and large volumes of basalt flushing through the crust from depth overprint their chemical signatures. This process will only maintain monotonous activity while the volume of melt entering the crust is high, raising the possibility of transitions to more silicic activity given a decrease in the crustal melt flux.
Chemoprotective effects of inositol hexaphosphate against cyclophosphamide-induced testicular damage in rats
Authors: Alkhalaf Maha I. , Alansari Wafa S. , Alshubaily Fawzia A. , Alnajeebi Afnan M. , Eskandrani Areej A. , Tashkandi Manal A. , Babteen Nouf A.
Published: July 2020

Cyclophosphamide (CP) is commonly used as an anticancer agent but has been associated with high toxicity in several animal organs, including the testes. Inositol hexaphosphate (IP6) is a polyphosphorylated carbohydrate that is present in foods with high fibre contents and has a wide range of essential physiological and pathological activities. Thus, we estimated the defensive effects of IP6 against CP-related testicular toxicity in rats. Sperm counts, motilities, viabilities and abnormalities and levels of testosterone, luteinising hormone and follicle-stimulating hormone were evaluated. Testicle specimens were also processed for histological and biochemical analyses, including determinations of malondialdehyde, nitric oxide, total antioxidant capacity, alkaline phosphatase, acid phosphatase, gamma glutamyl transferase, ß-glucuronidase, c-reactive protein, monocyte chemoattractant protein and leukotriene-4 and in comet assays. CP treatments were associated with deleterious histopathological, biochemical and genetic changes in rat testicles, and these were ameliorated by IP6 supplements in drinking water.
Objective quantification and spatial mapping of cataract with a Shack-Hartmann wavefront sensor
Authors: Holló Csaba Tamás , Miháltz Kata , Kurucz Máté , Csorba Anita , Kránitz Kinga , Kovács Illés , Nagy Zoltán Zsolt , Erdei Gábor
Published: July 2020

Based on wavefront sensor images an objective and quantitative method is presented for characterising cataract. By separating direct and scattered light in the focal plane of the microlenses, the new procedure is able to make two-dimensional maps of the spatial variation of scattering properties in the crystalline lens, and also provides a single figure descriptive for the whole eye. The developed evaluation algorithm successfully quantifies cataract, especially that of nuclear type. To demonstrate its operation, a custom-built measurement setup was constructed using a Shack-Hartmann wavefront sensor with $$40 \times 32$$40×32 microlenses to capture 12-bit images of the pupil plane, and a superluminescent diode of 830 nm wavelength as a light source. Slit-lamp clinical measurements served as reference for calibration and to estimate the accuracy of the new method. The tests were carried out on 78 eyes with cataract in different progression state ranging from healthy to above 5 on the LOCS III scale. The residual error of the calibration (i.e. the standard deviation of difference between clinical reference and our algorithmic characterisation) turned out to be $$\pm 0.29$$±0.29 category on the LOCS III N scale, which approximates the $$\pm 0.33$$±0.33 precision of classic cataract measurements carried out with the greatest care.
Adipocytes protect fibroblasts from radiation-induced damage by adiponectin secretion
Authors: Kosmacek Elizabeth A. , Oberley-Deegan Rebecca E.
Published: July 2020

Prostate and colon cancers are among the most common cancers diagnosed annually, and both often require treatment with radiation therapy. Advancement in radiation delivery techniques has led to highly accurate targeting of tumor and sparing of normal tissue; however, in the pelvic region it is anatomically difficult to avoid off-target radiation exposure to other organs. Chronically the effects of normal urogenital tissue exposure can lead to urinary frequency, urinary incontinence, proctitis, and erectile dysfunction. Most of these symptoms are caused by radiation-induced fibrosis and reduce the quality of life for cancer survivors. We have observed in animal models that the severity of radiation-induced fibrosis in normal tissue correlates to damaged fat reservoirs in the pelvic region. We hypothesize that adipocytes may secrete a factor that prevents the induction of radiation-associated fibrosis in normal tissues. In these studies we show that the adipokine, adiponectin, is secreted by primary mouse adipocytes and protects fibroblasts from radiation-induced cell death, myofibroblast formation, and senescence. Further, we demonstrated that adiponectin does not protect colorectal or prostate cancer cells from radiation-induced death. Thus, we propose that adiponectin, or its downstream pathway, would provide a novel target for adjuvant therapy when treating pelvic cancers with radiation therapy.
Focal dynamic thermal imaging for label-free high-resolution characterization of materials and tissue heterogeneity
Authors: O’Brien Christine M. , Meng Hongyu , Shmuylovich Leonid , Carpenter Julia , Gogineni Praneeth , Zhang Haini , Bishop Kevin , Mondal Suman B. , Sudlow Gail P. , Bethea Cheryl , Bethea Clyde , Achilefu Samuel
Published: July 2020

Evolution from static to dynamic label-free thermal imaging has improved bulk tissue characterization, but fails to capture subtle thermal properties in heterogeneous systems. Here, we report a label-free, high speed, and high-resolution platform technology, focal dynamic thermal imaging (FDTI), for delineating material patterns and tissue heterogeneity. Stimulation of focal regions of thermally responsive systems with a narrow beam, low power, and low cost 405 nm laser perturbs the thermal equilibrium. Capturing the dynamic response of 3D printed phantoms, ex vivo biological tissue, and in vivo mouse and rat models of cancer with a thermal camera reveals material heterogeneity and delineates diseased from healthy tissue. The intuitive and non-contact FDTI method allows for rapid interrogation of suspicious lesions and longitudinal changes in tissue heterogeneity with high-resolution and large field of view. Portable FDTI holds promise as a clinical tool for capturing subtle differences in heterogeneity between malignant, benign, and inflamed tissue.
Probing magnon–magnon coupling in exchange coupled Y$$_3$$Fe$$_5$$O$$_{12}$$/Permalloy bilayers with magneto-optical effects
Authors: Xiong Yuzan , Li Yi , Hammami Mouhamad , Bidthanapally Rao , Sklenar Joseph , Zhang Xufeng , Qu Hongwei , Srinivasan Gopalan , Pearson John , Hoffmann Axel , Novosad Valentine , Zhang Wei
Published: July 2020

We demonstrate the magnetically-induced transparency (MIT) effect in Y$$_3$$

3

Fe$$_5$$

5

O$$_{12}$$

12

(YIG)/Permalloy (Py) coupled bilayers. The measurement is achieved via a heterodyne detection of the coupled magnetization dynamics using a single wavelength that probes the magneto-optical Kerr and Faraday effects of Py and YIG, respectively. Clear features of the MIT effect are evident from the deeply modulated ferromagnetic resonance of Py due to the perpendicular-standing-spin-wave of YIG. We develop a phenomenological model that nicely reproduces the experimental results including the induced amplitude and phase evolution caused by the magnon–magnon coupling. Our work offers a new route towards studying phase-resolved spin dynamics and hybrid magnonic systems.
Screening of Parkinsonian subtle fine-motor impairment from touchscreen typing via deep learning
Authors: Iakovakis Dimitrios , Chaudhuri K. Ray , Klingelhoefer Lisa , Bostantjopoulou Sevasti , Katsarou Zoe , Trivedi Dhaval , Reichmann Heinz , Hadjidimitriou Stelios , Charisis Vasileios , Hadjileontiadis Leontios J.
Published: July 2020

Fine-motor impairment (FMI) is progressively expressed in early Parkinson’s Disease (PD) patients and is now known to be evident in the immediate prodromal stage of the condition. The clinical techniques for detecting FMI may not be robust enough and here, we show that the subtle FMI of early PD patients can be effectively estimated from the analysis of natural smartphone touchscreen typing via deep learning networks, trained in stages of initialization and fine-tuning. In a validation dataset of 36,000 typing sessions from 39 subjects (17 healthy/22 PD patients with medically validated UPDRS Part III single-item scores), the proposed approach achieved values of area under the receiver operating characteristic curve (AUC) of 0.89 (95% confidence interval: 0.80–0.96) with sensitivity/specificity: 0.90/0.83. The derived estimations result in statistically significant ($$p<0.05$$

p
<
0.05

) correlation of 0.66/0.73/0.58 with the clinical standard UPDRS Part III items 22/23/31, respectively. Further validation analysis on 9 de novo PD patients vs. 17 healthy controls classification resulted in AUC of 0.97 (0.93–1.00) with 0.93/0.90. For 253 remote study participants, with self-reported health status providing 252.000 typing sessions via a touchscreen typing data acquisition mobile app (iPrognosis), the proposed approach predicted 0.79 AUC (0.66–0.91) with 0.76/0.71. Remote and unobtrusive screening of subtle FMI via natural smartphone usage, may assist in consolidating early and accurate diagnosis of PD.
Distributed 2D temperature sensing during nanoparticles assisted laser ablation by means of high-scattering fiber sensors
Authors: Ashikbayeva Zhannat , Aitkulov Arman , Jelbuldina Madina , Issatayeva Aizhan , Beisenova Aidana , Molardi Carlo , Saccomandi Paola , Blanc Wilfried , Inglezakis Vassilis J. , Tosi Daniele
Published: July 2020

The high demand in effective and minimally invasive cancer treatments, namely thermal ablation, leads to the demand for real-time multi-dimensional thermometry to evaluate the treatment effectiveness, which can be also assisted by the use of nanoparticles. We report the results of 20-nm gold and magnetic iron oxide nanoparticles-assisted laser ablation on a porcine liver phantom. The experimental set-up consisting of high-scattering nanoparticle-doped fibers was operated by means of a scattering–level multiplexing arrangement and interrogated via optical backscattered reflectometry, together with a solid-state laser diode operating at 980 nm. The multiplexed 2-dimensional fiber arrangement based on nanoparticle-doped fibers allowed an accurate superficial thermal map detected in real-time.