bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploration of the Regulatory Effects of PSORI-CM01 and Yin Xie Ling on microRNAs in the Peripheral Blood Monocytes of Patients with Blood Stasis Syndrome of Psoriasis Vulgaris
Authors: Lu Yue , Qi Yao , Li Li , Yan Yuhong , Wei Jianan , Yao Danni , Deng Hao , Deng Jingwen , Ye Shuyan , Chen Haiming , Chen Qubo , Gao Hengjun , Han Ling , Lu Chuanjian
Published: August 2020

Background: Traditional Chinese medicines (TCMs) have been used to manage psoriasis since thousands of years and are associated with advantages such as long remission period and few side effects. PSORI-CM01 is a clinical TCM formula for treating the blood stasis syndrome of psoriasis vulgaris. It is optimised from Yin Xie Ling, a herbal preparation formulated by the Chinese national medical master Xuan Guowei. In the present study, we used microRNA (miRNA) chip technology to analyse the effects of PSORI-CM01 and Yin Xie Ling on miRNA differential expression in the peripheral blood monocytes of patients with blood stasis syndrome of psoriasis vulgaris.Methods: Blood samples collected from healthy subjects and patients with blood stasis syndrome of psoriasis vulgaris were obtained from the Department of Dermatology in the Guangdong Provincial Hospital of Chinese Medicine. These samples were obtained before and after treatment with PSORI-CM01 or Yin Xie Ling. Differentially expressed miRNAs were analysed by performing miRNA expression microarray to evaluate the mechanisms underlying the effects of the two formulations.Results: It was found that hsa-miR-3184-3p, hsa-miR-3653-3p, hsa-miR-20a-3p, hsa-miR-5681b, and hsa-miR-26a-5p were differentially expressed between the PSORI-CM01 and healthy control groups as well as between the Yin Xie Ling and healthy control groups before treatment, without any significant differences in their expression after treatment. The results revealed that the therapeutic effect of PSORI-CM01 on psoriasis was mediated mainly via the regulation of the adherens junction, thyroid hormone signalling, and proteoglycans in cancer pathways, whereas that of Yin Xie Ling was mediated mainly via the regulation of the endoplasmic reticulum protein processing, Hippo signalling, adherens junction, thyroid hormone signalling, cell cycle, proteoglycans in cancer, p53 signalling, oocyte meiosis, and unsaturated fatty acid biosynthesis pathways.Conclusions: The findings demonstrate that PSORI-CM01 and Yin Xie Ling are beneficial for treating blood stasis syndrome of psoriasis vulgaris through the regulation of hsa-miR-20a-3p and hsa-miR-3184-3p expression and, consequently, through the adherens junction, thyroid hormone signalling, and proteoglycans in cancer pathways.Trial registration: Registration number, ChiCTR-TRC-14005185; Date of registration, 8 August 2014.
BTK Inhibitors Differentially Induce Apoptosis but Similarly Suppress Chemotaxis and Lipid Accumulation in Mantle Cell Lymphoma
Authors: Liu Zhuojun , Liu Jia , Zhang Tainming , Li Lin , Zhang Shuo , Jia Hao , Xia Yuanshi , Shi Mingxia , Zhang Jing , Yue Shuhua , Chen Xiaofang , Yu Jian
Published: August 2020

Background: The more selective second-generation BTK inhibitors (BTKis) Acalabrutinib and Zanubrutinib and the first-generation BTK inhibitor (BTKi) Ibrutinib are highlighted by their clinical effectiveness in mantle cell lymphoma (MCL), however, similarities and differences of their biological and molecular effects on anti-survival of MCL cells induced by these BTKis with distinct binding selectivity against BTK remain largely unknown. Methods: AlamarBlue assays were performed to define cytotoxicity of BTKis against MCL cells, Jeko-1 and Mino. Cleaved PARP and caspase-3 levels were examined by immunoblot analysis to study BTKi-induced apoptotic effects. Biological effects of BTKis on MCL-cell chemotaxis and lipid droplet (LD) accumulation were examined in Jeko-1, Mino and primary MCL cells via Transwell and Stimulated Raman scattering imaging analysis respectively. Enzyme-linked immunoassays were used to determine CCL3 and CCL4 levels in MCL-cell culture supernatants. RNA-seq analyses identified BTKi targets which were validated by quantitative RT-PCR (qRT-PCR) and immunoblot analysis. Results: Acalabrutinib and Zanubrutinib induced moderate apoptosis in Ibrutinib high-sensitive JeKo-1 cells and Ibrutinib low-sensitive Mino cells, which was accompanied by cleaved PARP and caspase-3. Such effects might be caused by the stronger ability of Ibrutinib to upregulate the expression of pro-apoptotic genes, such as HRK, GADD45A , and ATM , in JeKo-1 cells than in Mino cells, and the expression of such apoptotic genes was slightly changed by Acalabrutinib and Zanubrutinib in both JeKo-1 and Mino cells. Further, Acalabrutinib, Zanubrutinib and Ibrutinib reduced MCL-cell chemotaxis with similar efficiency, due to their similar abilities to downmodulate chemokines, such as CCL3 and CCL4. Also, these three BTKis similarly suppressed MCL-cell LD accumulation via downregulating lipogenic factors, DGAT2, SCD, ENPP2 and ACACA without significant differences. Conclusion: BTKis demonstrated differential capacities to induce MCL-cell apoptosis due to their distinct capabilities to regulate the expression of apoptosis-related genes, and similar biological and molecular inhibitory effects on MCL-cell chemotaxis and LD accumulation.
The wastewater microbiome: a novel insight for COVID-19 surveillance
Authors: Gallardo-Escárate Cristian , Valenzuela-Muñoz Valentina , Núñez-Acuña Gustavo , Valenzuela-Miranda Diego , Castellón Francisco , Benavente-Cartes Bárbara , Sáez-Vera Constanza , Urrutia Homero , Novoa Beatriz , Figueras Antonio , Roberts Steven
Published: August 2020

Wastewater-Based Epidemiology is a tool to face and mitigate COVID-19 outbreaks by evaluating conditions in a specific community. This study aimed to analyze the microbiome profiles using nanopore technology for full-length 16S rRNA sequencing in wastewater samples collected from a penitentiary (P), a residential care home (RCH), and a quarantine or health care facilities (HCF). The HCF microbiome was strongly associated with enteric bacteria previously reported in patients with chronic disease and psychological disorders. During the study, the wastewater samples from the RCH and the P were negative for SARS-CoV-2 based on qPCRs, except during the fourth week when was detected. Unexpectedly, the wastewater microbiome from RCH and P prior to week four was correlated with the samples collected from the HCF, suggesting a core bacterial community is expelled from the digest tract of individuals infected with SARS-CoV-2. We provide novel evidence that the wastewater microbiome associated with gastrointestinal manifestations appears to precede the SARS-CoV-2 detection in sewage. This finding suggests that the wastewaters microbiome can be applied as an indicator of community-wide SARS-CoV-2 surveillance.
25-Gauge anterior vitrectomy via the scleral flap in phacoemulsification combined with trabeculectomy for glaucoma and cataract with extremely shallow anterior chamber
Authors: Shao Hui , Wu Bole , Ye Xinming , Meng Yiyu
Published: August 2020

Objective: To observe the safety and efficacy of a surgical technique of 25-gauge anterior vitrectomy via scleral flap in phacoemulsification combined with trabeculectomy for glaucoma and cataract with extremely shallow anterior chamber.Methods: This was a retrospective study composed with 18 eyes of 18 patinets（8 males and 10 females）, including 11 eyes with acute angle closure glaucoma, 8 eyes with lens subluxation combined with glaucoma. All patients underwent phacoemulsification, intraocular lens (IOL) implantation, trabeculectomy, and anterior vitrectomy via the scleral flap in cases where conservative managements cannot control intraocular pressure (IOP). The main outcomes were best corrected visual acuity (BCVA), anterior chamber depth (ACD), IOP, slit lamp microscopic examinations, medications, fundus examinations and complications.Results: The average axial length (AL) was 21.5 ± 0.6 mm (range: 20.0 to 23.2 mm). Mean age was 62.3 ± 7.9 years old (range: 46 to 73 years). Mean ACD increased statistically significant from 0.78 ± 0.43 mm to 2.89 ± 0.41 mm 1 week after surgery (P<0.001). Mean IOP decreased significantly from 43.28 ± 9.38 mmHg to 16.72 ± 6.28 mmHg (P<0.001). There were no serious complications occurred, such as endophthalmitis, retinal detachment, suprachoroidal hemorrhage, corneal decompensation and malignant glaucoma.Conclusions: 25-Gauge anterior vitrectomy via the scleral flap was a safe and effective technology in glaucoma combined with cataract with extremely shallow anterior chamber.
Clinical Efficacy of a Micro-locking Plate Vertical or Parallel Technique for Treatment of Dubberley B-type Capitellar Fractures
Authors: Lu Yao , Fu Lei , Liu Deyin , Xu Yibo , Xu Liping , Song Zhe , Fan Shan , Wang Qian , Sun Liang , Xue Hanzhong , Li Zhong , Zhang Kun , Ma Teng , Ren Cheng
Published: August 2020

ObjectiveTo evaluate the clinical efficacy of micro-locking plate through vertical or parallel technique for treatment of Dubberley B-type capitellar fractures.MethodsA retrospective analysis was performed in 24 patients (seventeen males and seven females, with an average age of 44.9 years, range from 19 to 75 years) with capitellar fractures that were treated with micro-locking plate using vertical or parallel technique between January 2016 to January 2019. The inclusion criteria include closed capitellar fracture, anterior elbow joint movement normal before injury, recent capitellar fracture with times of injury within past 3 weeks. Fractures classified according to Dubberley included 4 cases of type IB, 8 cases of type IIB and 12 cases of type IIIB. Radiographic evaluation was performed. Surgery time, blood lose, range of motion of the elbow, forearm rotation and complications was recorded. Elbow joint function was evaluated by Mayo Elbow Performance Score (MEPS).ResultsThe mean follow-up period was 19.6 months (range, 12–36 months). The average clinical healing time for fractures was 11.2 ± 3.2 weeks (range, 8–20 weeks). Fracture united in all patients. Two patients showed slight delayed union, but union was achieved eventually. The mean time from injury to surgery was 6.3 ± 3.1 days (range, 2–15 days). The average surgical time was 68.1 ± 11.5 minutes(range, 50–90 minutes), and the mean blood loss was 75.2 ± 26.5 mL༈range, 40–120 mL). The mean range of flexion was 122.5°±10.5°༈range, 95°-140°). The mean range of extension was 8.5°±5.8°༈range, 0°-20°). The mean range of pronation was 79.7°±8.0°༈range, 65°-90°). The mean range of supination was 80.5°±7.1°༈range,60°-90°). The mean MEPS at final follow-up was 89.8 ± 9.0 (range, 60–100). Based on the MEPS, 18 (75%) patients had excellent, 5 (20.8%) patients had good and 1 (4.2%) patient had fair. None of the 24 patients suffered vascular or nerve injury. One patient showed superficial infection, which terated with surgical dressing.Conclusions The micro-locking plate accroding to vertical or parallel technique is an excellent method for Dubberley B-type capitellar fractures.
Strategies for Involving Patients and the Public in Scaling-Up Initiatives in Health and Social Services: Protocol for a Two-Pong Study
Authors: Charif Ali Ben , Plourde Karine V. , Guay-Belanger Sabrina , Zomahoun Hervé Tchala Vignon , Gogovor Amédé , Straus Sharon , Beleno Ron , Kathner Kathy , Mclean Robert K.D. , Milat Andrew J. , Wolfenden Luke , Paquette Jean-Sébastien , Geiger Friedemann , Légaré France
Published: August 2020

Background: The scale-up of evidence-based innovations is required to reduce waste and inequities in health and social services (HSS). However, it often tends to be a top-down process initiated by policy-makers, and the values of the intended beneficiaries are forgotten. Involving multiple stakeholders including patients and the public in the scaling-up process is thus essential but highly complex. We propose to identify relevant strategies for meaningfully and equitably involving patients and the public in the science and practice of scaling up in HSS.Methods: Design: We will adapt our overall method from the RAND Appropriateness Method. Following this, we will perform a two-component study design (knowledge synthesis and Delphi study) grounded in an integrated knowledge translation (iKT) approach. This approach involves extensive participation of a network of stakeholders interested in patient and public involvement (PPI) in scaling up and a multidisciplinary steering committee. Knowledge synthesis: We will conduct a systematic scoping review following the methodology recommended in the Joanna Briggs Institute Reviewers Manual. We will use the following eligibility criteria: 1) Participants - any stakeholder involved in creating or testing a strategy for PPI; 2) Intervention - any PPI strategy proposed for scaling-up initiatives; 3) Comparator - no restriction; 4) Outcomes: any process or outcome metrics related to PPI; and 5) Setting - HSS. We will search electronic databases (e.g., MEDLINE, Sociological Abstract), hand searching relevant websites, screen the reference lists of included records, and consult experts in the field. Two reviewers will independently select and extract eligible studies. We will summarize data quantitatively and qualitatively and report results using the PRISMA extension guidelines. Delphi study: We will conduct an online Delphi survey to achieve consensus on the relevant strategies for PPI in scaling-up initiatives in HSS. Participants will include stakeholders from low-, middle-, and high-income countries. We anticipate that three rounds will allow an acceptable degree of agreement on research priorities.Discussion: Our findings will advance understanding of how to meaningfully and equitably involve patients and the public in scaling-up initiatives for sustainable HSS.Registration: We registered this protocol with the Open Science Framework on August 19, 2020 (https://osf.io/zqpx7/).
WITHDRAWN: Ultra-Precision Polishing Method of Polymer-Derived Amorphous SiAlCN Ceramics
Authors: Chen Yigao , Cao Yejie , Wang Yiguang
Published: August 2020

The amorphous and heterogeneous microstructures of polymer-derived ceramics (PDCs) make it challenging to uniformly polish them without the appearance of obvious polishing defects. In this study, the surface characteristics of polymer-derived silicoaluminum carbonitride (SiAlCN) ceramics were investigated after mechanical polishing and chemical–mechanical polishing (CMP). The results showed the generation of polishing defects including scratches, pits, and comet tails after mechanical polishing; nonetheless, the surface quality was largely improved after CMP, providing an ultra-smooth surface (Ra = ~0.25 nm). The evolution of the surface morphology was characterized in detail and X-ray photoelectron spectroscopy was used to analyze the characteristics of chemical bond before and after the CMP. The polishing slurry dominated the corrosion process during CMP, inducing the oxidation of the SiCxNy phase; nonetheless, the so-formed oxidation products and carbon phase were removed by mechanical action. The obtained ultra-high smooth surface can promote practical application in the field of PDCs-based sensors.
Serum D-dimer level as a biomarker for identifying patients with isolated injury to prevent unnecessary whole-body computed tomography in blunt trauma care
Authors: Nakama Rakuhei , Yamamoto Ryo , Izawa Yoshimitsu , Tanimura Keiichi , Mato Takashi
Published: August 2020

Background Unnecessary whole-body computed tomography (CT) may lead to excess radiation exposure. Serum D-dimer levels have been reported to correlate with injury severity. We examined the predictive value of serum D-dimer level for identifying patients with isolated injury that can be diagnosed with selected-region CT rather than whole-body CT.Methods This single-center retrospective cohort study included patients with blunt trauma (2014–2017). We included patients whose serum D-dimer levels were measured before they underwent whole-body CT. “Isolated” injury was defined as injury with Abbreviated Injury Scale (AIS) score ≤ 5 to any of five regions of interest or with AIS score ≤ 1 to other regions, as revealed by a CT scan. A receiver operating characteristic curve (ROC) was drawn for D-dimer levels corresponding to isolated injury; the area under the ROC (AUROC) was evaluated. Sensitivity, specificity, positive predictive value, and negative predictive value were calculated for several candidate cut-off values for serum D-dimer levels.Results Isolated injury was detected in 212 patients. AUROC was 0.861 (95% confidence interval [CI]: 0.815–0.907) for isolated injury prediction. Serum D-dimer level ≤ 2.5 µg/mL was an optimal cutoff value for predicting isolated injury with high specificity (100.0%) and positive predictive value (100.0%). Approximately 30% of patients had serum D-dimer levels below this cutoff value.Conclusion D-dimer level ≤ 2.5 µg/mL had high specificity and high positive predictive value in cases of isolated injury, which could be diagnosed with selected-region CT, reducing exposure to radiation associated with whole-body CT.
Role of Human Pegivirus Infections in Whole P. falciparum Sporozoite Vaccination and Controlled Human Malaria Infection in African Volunteers
Authors: Tumbo Anneth-Mwasi , Schindler Tobias , Dangy Jean-Pierre , Orlova-Fink Nina , Bieri Jose Raso , Mpina Maximillian , Milando Florence A. , Juma Omar , Hamad Ally , Nyakarungu Elizabeth , Chemba Mwajuma , Mtoro Ali , Ramadhan Kamaka , Olotu Ally , Makweba Damas , Mgaya Stephen , Stuart Kenneth , Perreau Matthieu , Stapleton Jack T. , Jongo Said , Hoffman Stephen L. , Tanner Marcel , Abdulla Salim , Daubenberger Claudia
Published: August 2020

Background: Diverse vaccination outcomes and protection levels pose a serious challenge to the development of an effective malaria vaccine. Co-infections are among many factors associated with immune dysfunction and sub-optimal vaccination outcomes. Chronic, asymptomatic viral infections can contribute to the modulation of vaccine efficacy through various mechanisms. Human Pegivirus-1 (HPgV-1) persists in immune cells thereby potentially modulating immune responses. We investigated whether Pegivirus infection influences vaccine-induced responses and protection in African volunteers undergoing whole P. falciparum sporozoites-based malaria vaccination and controlled human malaria infections (CHMI). Methods: HPgV-1 prevalence was quantified by RT-qPCR in plasma samples of 96 individuals before, during and post vaccination with PfSPZ Vaccine in cohorts from Tanzania and Equatorial Guinea. The impact of HPgV-1 infection was evaluated on (1) systemic cytokine and chemokine levels measured by Luminex, (2) PfCSP-specific antibody titers quantified by ELISA (3) asexual blood stage parasitemia and pre-patent periods with HPgV-1 infection status and (4) HPgV-1 RNA levels upon asexual blood stage parasitemia induced by CHMI. Results: The prevalence of HPgV-1 was 29.2% (28/96) and sequence analysis of the 5`UTR and E2 region revealed the predominance of genotypes 1, 2 and 5 in the positive volunteers. HPgV-1 infection was associated with elevated systemic levels of IL-2 and IL-17A. Comparable vaccine-induced anti-PfCSP antibody titers, asexual blood stage multiplication rates and pre-patent periods were observed in HPgV-1 positive and negative individuals. However, higher level of protection was detected in the HPgV-1 positive group (62.5%) than negative one (51.6%) following CHMI. Overall, HPgV-1 viremia levels were not significantly altered after CHMI. Conclusions: Although HPgV-1 infection did not alter vaccine-elicited levels of PfCSP-specific antibody responses and parasite multiplication rates, an ongoing infection appears to improve some degree of protection against CHMI in PfSPZ-vaccinated individuals. This is likely through modulation of immune system activation and systemic cytokines as higher levels of IL-2 and IL17A were observed in HPgV-1 infected individuals. CHMI is safe and well tolerated in HPgV-1 infected individuals. Identification of cell types and mechanisms of both silent and productive infection in individuals will help to unravel the biology of this widely present but largely under-researched virus.
Informal Economy and Gender Inequality,
Authors: Joseph Tonuchi Emmanuel , Nevo Chinedu Miracle , Nwolisa Chinyere Ugonwa
Published: August 2020

The COVID-19 pandemic ravaging the global economy has exposed not just the dominant nature of informal sector in Africa economy but also the vulnerability of the women in the continent. Thus, this study investigates the relationship between gender inequality and growth of the informal sector in Africa. Using Generalised Least Square Approach, it was discovered that gender inequality indices such as equality between male and female in getting job in the formal economy under same or similar circumstances have significant impact in the growth of the informal economy in the continent. The study concludes that if adequate effort is not made to address the gender inequality in the continent especially in formal employment that have forced many females to informal sector employment, the objective of achieving inclusive growth and development in the continent might not be realistic. The study therefore recommends that the UN and other international organisations should compensate the African women by giving the women at least 60 percent chance in all their employment as a way of increasing the economic and political power of the women in the continent.
Longitudinal proteomic profiling reveals increased early inflammation and sustained apoptosis proteins in severe COVID-19
Authors: Haljasmägi Liis , Salumets Ahto , Rumm Anna Pauliina , Jürgenson Meeri , Krassohhina Ekaterina , Remm Anu , Sein Hanna , Kareinen Lauri , Vapalahti Olli , Sironen Tarja , Peterson Hedi , Milani Lili , Tamm Anu , Hayday Adrian , Kisand Kai , Peterson Pärt
Published: August 2020

SARS-CoV-2 infection risks developing into life-threatening COVID-19 disease. Whereas age, hypertension, and chronic inflammatory conditions are risk factors, underlying host factors and markers for disease severity, e.g. requiring intensive care unit (ICU) treatment, remain poorly defined. To this end, we longitudinally profiled blood inflammation markers, antibodies, and 101 plasma proteins of hospitalized COVID-19 patients who did or did not require (ICU admission. Whereas essentially all patients displayed SARS-CoV-2-specific antibodies and virus-neutralization capacity within 12-15 days, a rapid, mostly transient upregulation of selective inflammatory markers including IL-6, CXCL10, CXCL11, IFNg, IL-10, and monocyte-attracting CCL2, CCL7 and CCL8, was particularly evident in ICU patients. In addition, there was consistent and sustained upregulation of apoptosis-associated proteins CASP8, TNFSF14, HGF, and TGFB1, with HGF discriminating between ICU and non-ICU cohorts. Thus, COVID-19 is associated with a selective inflammatory milieu within which the apoptotic pathway is a cardinal feature with potential to aid risk-based patient stratification.
Knowledge, Attitude and Practice of Sexual and Reproductive Health Among Older Adolescent Girls in Bangladesh: An Institution-Based Cross-Sectional Study
Authors: Zakaria Muhammad , Xu Junfang , Karim Farzana , Mazumder Subarna , Cheng Feng
Published: August 2020

Background: Improving the sexual and reproductive health (SRH) of adolescent girls is one of the primary agenda of the Sustainable Development Goals (SDGs). Adequate and accurate knowledge, favorable attitude, safe behavior, and regular practice contribute to the adolescent girls’ SRH, maternal, and child health. In the background, this study aims to explore the level of knowledge, attitudes, and practices (KAP) of SRH among college-going older adolescent girls in Chittagong district, Bangladesh. Methods: An institution-based cross-sectional study was conducted in four colleges among the older adolescent girls age group of 16-17 (N = 792) attending a higher secondary grade in Chittagong district. Data were collected using a structured and self-administered questionnaire. Descriptive statistics and multiple linear regression analyses were used to summarize the SRH-related KAP and identify the associated factors, respectively. Results: 62% of study participants had the awareness that both physical and psychological changes occur in the adolescence period, while only 36% knew that menstruation is not a disease. Besides, only 30% of adolescent girls were informed about taking birth control has no adverse effect on the sexual relationship of a couple. Moreover, only 40% of students were learned that HIV could not be spread through the mosquito and flea, whereas only 25% knew that transmission of HIV is unlikely from an infected person’s coughing and sneezing. Of older adolescent girls, 35% disagreed perfectly whether sexual education can lead to more sexual activity. Besides, more or less 20% of participants opined that adolescent girls should refrain from going outside even to the school, entering into the kitchen, touching anyone, brushing hair, and see in the mirror. Moreover, 52% of adolescent girls wanted to learn more details about SRH, while 48% felt timid and afraid of their puberty changes. Besides, 62.5% of adolescent girls reported their using cloth during the menstrual cycle instead of the sanitary pad. Standardized coefficients beta (β) and p value < .05 in linear regression analyses explored that adolescent girls’ study of science, urban residence, reading or watching SRH contents on the mass media appeared as the more significant predictors for outcome variables. Conclusion: Many problems related to SRH exist among older adolescent girls in Bangladesh, such as behaviors and social constraints associated with menstruation, myths, and assumptions, recognition of the value of awareness and knowledge of reproductive health. Therefore, strengthening SRH-related comprehensive education programs incorporating into the curriculum, effective use of mass media, and supplying behavioral change communication materials are essential.
Extended - spectrum beta - lactamase E. coli and K. pneumoniae urinary tract infections
Authors: Vachvanichsanong Prayong , McNeil Edward B , Dissaneewate Pornsak
Published: August 2020

Background: The prevalences of extended - spectrum beta - lactamase (ESBL) Escherichia coli ( E . coli ) and Klebsiella pneumoniae ( K . pneumoniae ) urinary tract infections (UTI) in children are increasing worldwide. We aimed to investigate the prevalence, clinical findings, impact and risk factors of ESBL E . coli / K . pneumoniae UTI.Methods: The medical records of children with UTI aged <15 years admitted to Prince of Songkla University Hospital were reviewed. Results: Theirty-seven boys and 46 girls had ESBL in 102 UTI episodes; 85 boys and 103 girls had non-ESBL in all of their 222 UTI episodes. The median age at presentation was 1.5 (0.7 - 4.8) years for the ESBL group and 1.3 (0.6 - 3.9) for the non-ESBL group (p=0.2). Age and gender were not significantly different between the two groups. The prevalence of ESBL rose between 2004 and 2008 before plateauing at around 30-40% per year. The prevalences in first and recurrent UTI were 27.3% and 46.5%, respectively (p=0.003). Fever prior to UTI diagnosis was found in 78.4% of episodes in the non-ESBL group and 61.8% of episodes in the ESBL group (p=0.003). Multivariate analysis, children without fever (OR=2.14, 95% CI: 1.23-3.74) and those with recurrent UTI (OR=2.67, 95% CI: 1.37 – 5.19) were more likely to have ESBL UTI. The presence of CAKUT had no effect on ESBL UTI. Conclusions: ESBL was found in one-third of E . coli / K . pneumoniae UTI episodes. No clinical condition nor imaging study could predict ESBL. Recurrent UTI was the only independent risk factor.
Pandemic-related PTSD symptoms and substance use among community-based adults
Authors: Currie Cheryl L.
Published: August 2020

Objectives: To examine: (1) the role of gender and socioeconomic status in pandemic-related post-traumatic stress disorder (PTSD) symptoms and substance use; (2) associations between probable PTSD and substance use, and (3) the supports needed to address these problems. Methods: Data were collected in June 2020 from 933 community-based adults in Alberta without a previous diagnosis of PTSD. The Primary Care PTSD Screen was adapted to assess pandemic-related PTSD symptoms. Participants were asked if alcohol or cannabis use had increased in the past month. Adjusted logistic regression models examined associations between probable PTSD and substance use. Results: Significantly more women (19%) than men (13%) met criteria for probable pandemic–related PTSD, while a similar percentage (13.5% of women, 13.0% of men) reported increased substance use during the pandemic. Adults with lower income, education, or pandemic-related job loss were more vulnerable to PTSD and substance use increases. Probable pandemic-related PTSD was associated with increased substance use for both women (OR = 2.2) and men (OR = 2.3) in adjusted models. Many adults (50% of women, 40% of men) indicated they needed support to address mental health or substance use during the pandemic, particularly from friends, a physician, and/or a counsellor. Conclusions: This study examined adults who had just experienced two months of increasing COVID-19 cases and containment measures. Findings suggest women and socioeconomically vulnerable adults may be in greater need of mental health supports, and that pandemic-related PTSD is an important consideration for interventions to reduce substance use among both women and men.
Synbiotic kefir lowered peroxisome proliferator activated receptor gamma (PPARγ) gene expression in rat fed high-fat and high-fructose diet
Authors: Nurliyani  , Harmayani Eni , Sunarti
Published: August 2020

Kefir is fermented milk product containing bacteria and yeast, whereas glucomannan from porang (Amorphophallus oncophyllus) tuber has known as prebiotic in vivo. Diets with a high fat and high sugar will stimulate metabolic syndrome. The objective of this study were to determine the effect of synbiotic kefir (goat milk kefir enriched with porang glucomannan) on blood glucose, hemoglobin A1c (HbA1c), free fatty acid (FFA), tumor necrosis factor alpha (TNF-α), gene expression of peroxisome proliferator activated receptor gamma (PPARγ), and insulin producing cells in rat fed high- fat and high- fructose (HFHF) diet. Rats were divided into 5 groups: normal; high fat high fructose (HFHF); HFHF + probiotic kefir; HFHF + synbiotic kefir; and HFHF + simvastatin. There was no significantly differences in plasma blood glucose in HFHF rat after treated with synbiotic kefir. However, synbiotic kefir could decrease HbA1c and plasma TNFα, and inhibit the increasing FFA in HFHF rats. Probiotic and synbiotic kefir could decrease gene expression of PPARγ2 in both of adipose and liver tissue in HFHF rats, but had no effect on total number of Langerhans islet and insulin producing cell. In conclusion, synbiotic kefir could ameliorate the health of rats in condition of high-fat and high-fructose diet, through decreasing in HbA1c, TNFα, and gene expression of PPARγ2 and also prevent the increasing of FFA. Therefore, synbiotic kefir containing porang glucomannan is expected to be a suggestion for the food industry to develop synbiotic-based functional foods which has the potential to improve metabolic syndrome
A new forecast model for fluctuating Pattern of Covid-19 spread in South Asia
Authors: Hussain Muhammad Arif , Zaheer Kashif Bin , Haroon Syed Muhammad , Kashif Amber Nehan
Published: August 2020

Exponential growths are observed in several natural phenomena, including cosmological expansion of the universe, nuclear fission, population growth, global viral spread, and financial markets fluctuations. Several deterministic and stochastic methods have been used for the modeling of dynamics of these phenomena. Global reliable data are essential to parameterise a defined model so as to enhance confidence in model outputs. Four-parameter logistic model comes out to be best forecast model for the total Covid-19 infected people in Pakistan and India. Five-parameter logistic model is best forecast model for Bangladesh. Simulation results revel that maximum total number of cases will be about 1.0, 0.6 and 0.25 million in India, Pakistan and Bangladesh respectively. Covid-19 infected rate will be closed to 11000, 8000 and 2000 cases per day during peak period in these countries.
A Comparative Study of Spinel ZnFe2O4 Ferrites Obtained via a Hydrothermal and a Ceramic Route: Structural and Magnetic Properties
Authors: Zhu Xuesong , Cao Chunxiang , Su Shubing , Xia A.L. , Zhang Huiyan , Li Hailing , Liu Zhiyuan , Jin Chuangui
Published: August 2020

The spinel ZnFe2O4 specimens were obtained via a hydrothermal and a ceramic method, respectively, and their structural and magnetic properties were comparatively studied. It was found that all the specimens exhibited a single-phase and mixed spinel structure. The magnetism of specimens synthesized via the hydrothermal method is obviously greater than that of specimen prepared via the ceramic method. This can be ascribed to the occupancy of Fe ions resulted from the loss of Zn during the hydrothermal process.
The Effects of Pneumococcal and Haemophilus Vaccines in Children Under 5 Years of Age on Severity of Illness and Deaths From Infections Other Than Those Conditions That the Vaccine is Designed to Prevent, and on All-cause Mortality and All-cause Hospitalisation: A Systematic Review Protocol
Authors: Rooney Darragh , Ledwidge Mark , Watson Chris , Gallagher Joe , Glynn Liam
Published: August 2020

BackgroundVaccination in children has reduced morbidity and mortality worldwide from infectious diseases. It has been suggested that vaccines have beneficial effects beyond the diseases they are designed to prevent. These are known as “non-specific effects” and include reductions in severity of illness and hospitalisation for illnesses other than those the vaccine is designed to prevent. This protocol will focus specifically on the non-specific effects of pneumococcal and haemophilus influenza vaccines in children under 5 years of age.MethodsWe will systematically search Medline, Embase, Cochrane Central Register of Controlled Trials, the European Union Clinical Trials Register and the clinicaltrials.gov databases using a broad range of search terms pertaining to pneumonia, morbidity, mortality and children to identify potentially relevant studies. These will be limited to randomised controlled trials, quasi randomised controlled trials and cohort studies in English. Two independent reviewers will conduct all levels of screening, data abstraction, and quality appraisal (using the Cochrane risk of bias tool). DiscussionOur results can be used by researchers and policy-makers to identify if there are non-specific effects of pneumococcal and haemophilus vaccines which should be explored further. The review will be also be of interest to patients and clinicians to determine if vaccines have beneficial effects beyond the illnesses they are designed to prevent.Systematic review registrationThe systematic review protocol is registered with the PROSPERO database, Registration number: CRD42020146640.
The characteristics of echocardiography in ICU patients and its relationship with the outcomes
Authors: Peng Qian-Yi , Liu Li-Xia , Zhang Qian , Zhu Ying , Zhang Hong-Min , Yin Wan-Hong , He Wei , Shang Xiu-Ling , Chao Yan-Gong , Lv Li-Wen , Wang Xiao-Ting , Zhang Li-Na
Published: August 2020

Background Echocardiography is widely used for bedside monitoring in ICU. We aimed to describe the characteristics of echocardiography manifestations in different groups of ICU patients, and the association with the outcomes.Methods This is a prospective multiple-center cohort study took place in 17 ICUs in China. A total of 1682 continuous adult patients admitted between August 31, 2017 to February 16, 2019 who had echocardiography performed within 24 hours in ICU were included. The echocardiography was performed and the echocardiography scores were calculated. Data was analyzed and compared between different outcome and primary indication groups.Results The overall 28-day mortality was 15.76%. Left ventricle enlargement and other echocardiogram abnormalities were more common in the non-survivors than the survivors. The EF value was lower, and the decrease in EF value was more frequent in the non-survivors than the survivors. The echocardiography score was significantly higher in the non-survivors than the survivors. The incidence of right ventricle enlargement and EF decrease was high in ICU patients (26.52% and 26.93% respectively). The independent risk factors for 28-day mortality was APACHE II, the length of MV, the length of ICU stay, oxygenation index, right ventricle wall thickening, IVC diameter, IVC variability, and the echocardiography score.Conclusions The incidence of cardiac dysfunction is high in ICU patients. The echocardiography score was significantly higher in the non-survivors than the survivors, and was an independent risk factor for 28-day mortality. Echocardiography is a convenient bedside monitoring method which deserves widely use in ICUs.Trial registration: Chinese Clinical Trial Registry (ChiCTR-DDD-17012391). Registered 2017.08.17. http://www.chictr.org.cn/showprojen.aspx?proj=21127.
Patterns of Antidepressant Prescriptions for the Treatment of Depression: A Scoping Review
Authors: Marasine Nirmal Raj , Sankhi Sabina , Lamichhane Rajendra , Dangi Nim Bahadur
Published: August 2020

Introduction: Depression is an important global public health problem due to its relatively high lifetime prevalence and significant disability caused by it. It is associated with functional impairment and high morbidity and mortality. Antidepressant medication is considered to be the best treatment option for depressionMethod: Literature searches (2000-2019) were performed using PubMed, HINARI and Google Scholar that analyze the pattern of prescription of antidepressants among the patients of depression. .Results: Females suffered from depression more than their male counterparts. Married people, housewives, lower income people, employees and highly educated people suffered more from depression. Selective Serotonin Reuptake Inhibitors (SSRI) like Sertraline, was found out to be the most preferred antidepressant among the depressive patients. Conclusion: Our Study suggested that out of five major antidepressant drugs being available for the treatment of depression, selective serotonin reuptake inhibitors are preferred over others because of their better side effect and tolerability profile.
Synthesis of nearly spherical AlN particles by an in-situ nitriding combustion route
Authors: Wei Zhilei , Li Kang , Ge Bangzhi , Guo Chaowei , Xia Hongyan , Guo Yajie , Shi Zhongqi
Published: August 2020

Spherical AlN powders with micrometer size have attracted great attention owing to their good fluidity and dispersity. However, the industrial preparation methods usually require high temperature and long soaking time, which lead to the high cost and limit the wide application of the products. Herein, nearly spherical AlN particles with the average size of 2.5 µm were successfully synthesized via an in-situ combustion synthesis method. The effect of N2 pressure, NH4Cl content and Al particle size on the combustion reaction procedure, phase composition and microstructure of the products was systematically investigated. The results showed that the decreased N2 pressure, increased NH4Cl content and Al particle size led to the decreasing of combustion temperature and speed, which further affected the morphology of the products. As a result, low N2 pressure (0.2 MPa), a small amount of NH4Cl (0.5 wt%) and fine Al particles (~ 2.5 µm) contributed to a moderate combustion temperature and facilitated the formation of nearly spherical AlN particles. In addition, based on the gas-releasing assisted quenching experiments and thermo-kinetic analysis, a two-step growth mechanism for the nearly spherical AlN particles was rationally proposed. The present method shows the advantages of low cost and high efficiency for preparing nearly spherical AlN particles, which can be used as raw materials for electronic substrates and fillers for packaging materials.
Rare disease awareness and perspectives of physicians in China: a questionnaire-based study
Authors: Li Xuefeng , Zhang Xiangyu , Zhang Shu , Lu Zijuan , Zhang Jianyong , Zhou Jincheng , Li Bingzhe , Ou Li
Published: August 2020

Background

There are over 16.8 million rare disease patients in China, representing a significant challenge for the healthcare system and society. Rare disease patients often experience delayed diagnosis, misdiagnosis, or improper treatment, which may be due to the lack of rare disease awareness among physicians.

Results

A total of 224 physicians from different hospitals in China participated in the questionnaire, and 9 rare disease experts were interviewed with open-ended questions. Most physicians (83.5%) were from Tertiary hospitals, which have over 500 beds. Only 5.3% of physicians were moderately or well aware of rare diseases. Most physicians (80.1%) had suspected their patients to have rare diseases less than 3 times. There was a strong support for special legislations for rare diseases and orphan drugs. Further, multinomial logistic regression (MLR) was used to determine whether hospitals, gender, and career length has an impact on perspectives and awareness. It was shown that male physicians were more likely to think newborn screening is important (p<0.05). The longer the career length is, the more likely physicians believe that their previous education has not provided sufficient information about rare diseases and that their hospital has paid enough attention to rare diseases. Physicians from Tertiary A hospitals were more likely to rate the affordability of orphan drugs high. In addition, nine experts believed that rare disease awareness is essential for early diagnosis and timely treatment. These experts also made recommendations on how to improve rare disease awareness through medical school education and continuing training.

Conclusions

Our study highlighted the importance of improving rare disease awareness among physicians in China. Recommendations about how to improve rare disease awareness in medical school education and establish an online ‘information hub’ are made for considerations of policy-makers.
Testing Flowering Perennial Plants in a Bermudagrass (Cynodon spp.) Lawn
Authors: Boyle Paige E. , Wisdom Michelle M. , Richardson Michael D.
Published: August 2020

Most pollinating insects require a season-long succession of floral resources to fulfill life-cycle requirements. Incorporating forbs into turfgrass sites may create a season-long sequence of flowers to support foraging pollinators. However, persistence of forbs in warm-season turfgrasses such as bermudagrass (Cynodon spp.) may be affected by the competitive nature of the turfgrass and routine management practices such as mowing. A 2-year study was conducted to evaluate seven forbs (Bellis perennis L., Lotus corniculatus L., Prunella vulgaris L., Trifolium fragiferum L. ‘Fresa’, Trifolium repens L. ‘Durana’ and ‘Resolute’, Trifolium subterraneum L.) for persistence and ability to produce floral resources for pollinating insects in a low maintenance bermudagrass lawn. Plugs of each species were incorporated into ‘Riviera’ bermudagrass in Apr. 2016. Vegetative cover, flower production, flowering period and pollinator foraging were assessed. Prunella vulgaris bloomed July through August and achieved 100% cover (0% bermudagrass) by 2017. Trifolium repens achieved a more balanced competitive density with the bermudagrass and produced flowers from June through August in both years. Trifolium fragiferum persisted over two growing seasons but only bloomed in 2017. Bellis perennis, Lotus corniculatus and Trifolium subterraneum did not persist. Pollinators were observed foraging on all persistent, flowering forbs, including Trifolium repens, Prunella vulgaris, and Trifolium fragiferum. Trifolium repens and Prunella vulgaris produced the most flowers and attracted the most pollinators.
Environmental politics and policy adaptation in China: the case of water sector reform
Authors: Moore Scott , Yu Winston
Published: August 2020

Much of the literature on China's successful adaptation to the policy challenges posed by economic development credits two principle approaches, gradualism and local experimentation. However, the extent to which these approaches aid policy adaptation to environmental policy challenges is less well explored. This article examines how these approaches have shaped policy adaptation in water resources management by presenting data on ambitious water policy reforms that are, to our knowledge, new to the English-language scholarly literature. While gradualism and local experimentation have aided in the adoption of economic mechanisms like water pricing reform and water rights trading to regulate water use, institutional reforms have been undermined by an over-reliance on central control and direction. This phenomenon, which we call hierarchy, constrains China's ability to address diffuse, inter-jurisdictional and multi-sectoral water management challenges like nonpoint source pollution, and may inhibit its ability to address similarly complex sustainable development challenges into the future.
INTERPRETAÇÃO DA PAISAGEM DO SEMIÁRIDO PIAUIENSE: PRÁTICA DE CAMPO NO MUNICÍPIO DE PRATA DO PIAUÍ E NA CIDADE DE PEDRAS
Authors: Sousa Francisco Wellington de Araújo , Costa Géssica Maria Mesquita Monteiro , Sousa Glécia Maria de Carvalho , Lima Iracilde Maria de Moura Fé
Published: August 2020

O presente artigo teve como objetivo caracterizar as paisagens de trechos visitados nas atividades de campo, em Prata do Piauí e na Cidade de Pedras, buscando identificar sua adequação, ou não, à classificação de espaços semiáridos, a partir dos critérios de tipos climáticos e cobertura vegetal utilizado pela literatura para definir a região semiárida brasileira. Os procedimentos metodológicos foram embasados na abordagem dialética que teve início com a discussão em sala de aula, em momentos de articulação dos roteiros de observação nos municípios citados.  Durante os trabalhos de campo utilizou-se do caráter descritivo-exploratório por meio de mapas, preenchimento de fichas, uso do aparelho Global Positioning System (GPS) e câmera fotográfica. Em laboratório fez-se uso do software Qgis para trabalhar os dados e informações levantados. Para alcançar o objetivo proposto, foi realizada a caracterização fisiográfica e a observação de forma comparativa, como subsídio à análise das paisagens locais em relação à conceituação de ambiente semiárido.
Mutations of mtDNA in some Vascular and Metabolic Diseases
Authors: Sazonova Margarita A. , Ryzhkova Anastasia I. , Sinyov Vasily V. , Sazonova Marina D. , Kirichenko Tatiana V. , Doroschuk Natalya A. , Karagodin Vasily P. , Orekhov Alexander N. , Sobenin Igor A.
Published: August 2020

Background:

The present review article considers some chronic diseases of vascular and metabolic
genesis, the causes of which may be mitochondrial dysfunction. Very often, in the long course of the disease,
complications may occur, leading to myocardial infarction or ischemic stroke and, as a result, death. In particular,
a large percentage of human deaths nowadays belongs to cardiovascular diseases, such as coronary heart
disease (CHD), arterial hypertension, cardiomyopathies, and type 2 diabetes mellitus.

Objective:

The aim of the present review was the analysis of literature sources, devoted to an investigation of a
link of mitochondrial DNA mutations with chronic diseases of vascular and metabolic genesis.

Results:

The analysis of literature indicates the association of the mitochondrial genome mutations with coronary
heart disease, type 2 diabetes mellitus, hypertension, and various types of cardiomyopathies.

Conclusion:

The detected mutations can be used to analyze the predisposition to chronic diseases of vascular and
metabolic genesis. They can also be used to create molecular-cell models necessary to evaluate the effectiveness
of drugs developed for the treatment of these pathologies. MtDNA mutations associated with the absence of diseases
of vascular and metabolic genesis could be potential candidates for gene therapy of the said diseases.