bluuebunny/crossref_metadata_2025 huggingface dataset:
Reducing burden from respiratory infections in refugees and immigrants: a systematic review of interventions in OECD, EU, EEA and EU-applicant countries
Authors: Lambert Jan-Frederic , Stete Katarina , Balmford James , Bockey Annabelle , Kern Winfried , Rieg Siegbert , Boeker Martin , Lange Berit
Published: March 2020

Background Respiratory diseases are a major reason for recently arrived migrants seeking health care in countries of arrival. The burden of respiratory diseases in refugees is exacerbated by poor living conditions and crowding. The lack of synthesised evidence and guideline-relevant information to reduce morbidity and mortality from respiratory infections endangers this population. Methods A systematic review of all controlled and observational studies assessing interventions targeting the treatment, diagnosis and management of respiratory infections in refugees and immigrants in OECD, EU, EEA and EU-applicant countries published between 2000 and 2019 in MEDLINE, CINAHL, PSYNDEX and Web of Science. Results Nine of 5779 identified unique records met our eligibility criteria. Seven studies reported an increase in vaccine coverage from 2% – 52% after educational multilingual interventions for respiratory-related childhood diseases (4 studies) and for influenza (5 studies). There was limited evidence in one study that hand sanitiser reduced rates of upper respiratory infections and when provided together with face masks also the rates of influenza-like-illness in a hard to reach migrant neighbourhood. In outbreak situations of vaccine-preventable diseases, secondary cases and outbreak hazards were reduced by general vaccination strategies early after arrival but not by serological testing after exposure (1 study). We identified evidence gaps regarding interventions assessing housing standards, reducing burden of bacterial pneumonia and implementation of operational standards in refugee care and reception centres. Conclusions Multilingual health literacy interventions should be considered to increase uptake of vaccinations in refugees and immigrants. Well designed controlled studies on housing and operational standards in refugee population early after arrival as well as adequate ways to gain informed consent for early vaccinations in mass housing is required to inform guidelines.
The efficacy of different treatment approaches for pediatric OSAHS patients with mandibular rethognathia: a study protocol for a multicenter randomized controlled trial
Authors: Li Yuanyuan , Wu Jiali , Guo Jinghan , Yu Liming , Wang Jing , Li Xiaoyan , Xu Shuhua , Zhu Min , Feng Jinqiu , Liu Yuehua
Published: March 2020

Background: Pediatric obstructive sleep apnea/hypopnea syndrome (OSAHS) is a multifactorial syndrome caused by many risk factors, such as craniofacial anomalies, adenotonsillar hypertrophy, obesity and airway inﬂammation. Although new treatment patterns have been recently proposed, treatment methods for children have continued to be particularly challenging and controversial. This randomized controlled trial was designed to investigate the efficacy of adenotonsillectomy and/or orthodontic treatment for mild OSAHS children with mandibular rethognathia.Methods: A sample of 352 children with mild OSAHS and mandibular rethognathia, aged between 7 and 10 years, will be enrolled in the study. They will be randomized into four groups – the drug treatment group, or the surgical treatment group, or the orthodontic treatment group, or the surgery and postoperative orthodontic group. After randomization they will receive treatments within 4 weeks. Outcome assessment will take place at points: (1) at baseline, (2) 7 months after the treatment starting point, (3) 12 months after the treatment starting point, (3) 24 months after the treatment starting point. The primary endpoint of the trial is the mean change of obstructive apnea/hypopnea index. Other endpoints will consist of the lowest oxygen saturation, apnea index, and hypopnea index assessed by polysomnogram, subjective symptoms (assessed by questionnaire OSA-20), cephalometric measurements and Morphologic analysis of upper airway.Discussion: The results of this study will provide valuable evidence for merits and long-term efficacy of different treatment approaches and contribute to facilitate the multidisciplinary treatment of pediatric OSAHS.Trial registration: Clinicaltrials.gov, ID: NCT03451318. Registered on 2 March 2018 (last update posted 19 April 2018).
Efficacy and safety of the extracorporeal shock wave therapy in patients with postherpetic neuralgia: study protocol of a randomized controlled trial
Authors: Chen Lu , Zhou Ruihao , Sun Fuguo , Weng Yan , Ye Ling , Yang Pingliang
Published: March 2020

Background: Postherpetic neuralgia (PHN) is one of the most common chronic neuropathic pain, which seriously affected the quality of the life due to the severity of pain and the poor response to the current treatment. The main management strategies of PHN include medication therapy and invasive interventional therapy. However, there are lots of side effects. It is meaningful to find another effective and safe treatment for PHN. Methods: A single-center, randomized, single-blind clinical trial will be held. A total of 98 participants will be randomly divided into control group and experimental group in a 1:1 ratio. Patients in control group will receive conventional treatment including medication therapy and invasive interventional therapy. The experimental group will be treated with extracorporeal shockwave therapy (ESWT) in addition to conventional therapy. The primary outcome is visual analogue scale (VAS), secondary outcome contains 36-item short-form health survey (SF-36), self-rating anxiety Scale (SAS), self-rating depression scale (SDS) and Pittsburgh sleep quality index (PSQI). Assessors who are blinded to the randomization will collect data during the intervention period at baseline, 1 weeks, 4 weeks, and 12 weeks in this study. The plasma levels of TNF-α、IL-6 in patients will be detected before and after ESWT to explore part of the biochemical mechanism of ESWT for the treatment of PHN. Discussion: This randomized controlled trial will be held to evaluate the actual effectiveness and safety of ESWT in patients with PHN, and thus provide clinical evidences for its application in the PHN management and explore the potential mechanism of this treatment. Trial registration: www.ChiCTR.org.cn, identifier: ChiCTR1900025828. Registered on 10th September 2019.
Yu Nu compound regulates autophagy and apoptosis through mTOR in vivo and vitro
Authors: He Caigu , Liu Guang , Zhuang Shuting , Zhang Jialin , Chen Yangtao , Li Hetian , Huang Zhengpin , Zheng Yanfang
Published: March 2020

Background: Yu Nu compound (YNJ) is a traditional Chinese medicine widely utilized to treat type 2 diebetes, which has been reported to regulate autophagy. Abnormal podocyte autophagy and apoptosis could result in podocyte loss in diabetics nephropathy (DN). The mechanism of Yu Nu compound in DN is still unclear. Therefore, the study aims to investigate how Yu Nu compound exerts effects on DN rats.Methods: GK rats were administered using YNJ with different dose once a day by gavage for 4 weeks. The renal cortex injury was observed by HE and electron microscope. Cell apoptosis of renal cortex was analyzed by tunnel staining. The mTOR, autophagy-related proteins and apoptosis-related proteins were detected by Western blot or Real-time PCR in vivo and vitro. MPC5 cells were exposed to high glucose (HG, 30mM) for 12h for simulating podocytes injury in DN. MPC5 cells were treated by serum containing YNJ with different dosage. Cell activities and apoptosis were respectively detected through CCK8 assay and Flow cytometry.Results: The results showed that the medium dose of YNJ has better effects on decreasing blood glucose and improving renal injury in GK rats, followed by decreasing mTOR levels. The autophagy levels were enhanced, accompanied with the increase of cell apoptosis in vivo and vitro. The decreasing endogenous mTOR could reverse the effects of YNJ on cell apoptosis and autophagy.Conclusion : the study suggested that YNJ reduced the excessive autophagy and suppressed apoptosis through regulating mTOR. The maintenance of normal basal autophagic activity possibly based on the effect of YNJ on multiple target was essential for maintaining podocytes function.
Time to recovery from Proteinuria and its related factors in patients with lupus nephritis
Authors: Aghdashi Miramir , Makhdomi Khadije , Rashidi Arash
Published: March 2020

Background lupus nephritis (LN) is a severe form of systemic lupus erythematosus (SLE) with renal involvement. It affects the kidneys in about 50% of SLE patients. The aim of this study was to assess the evaluation of proteinuria recovery time and its related factors associated with lupus nephritis patients in Urmia- Northwest of Iran.Methods A retrospective cohort study was carried out, in which medical records of 80 patients with systemic lupus nephritis referred to Imam Khomeini university hospital were reviewed. According to these records biopsy-proven renal disease has been progressed from September 2009 to September 2013. Proteinuria less than 0.5 g/24h was defined as proteinuria recovery. The time elapsed from the diagnosis of proteinuria to its recovery is considered as the duration of proteinuria recovery (month). The findings were analyzed by STATA11 statistical software.Results Mean age at diagnosis of lupus nephritis was 26.50±8.10 years (14-51 years). Mean creatinine level at the start of treatment was 1.20±0.61 mg/dl (0.5- 2.80). Proteinuria recovery time was 4 months for 25% of patients, 6 months for 50% of patients (median time) and 12 months for 75% of them. Higher class of LN had a trend toward 31% lower risk of proteinuria recovery (HR: 0.73, 95% CI 0.56 -0.96; P= 0.02), the expected risk is 1.94 times greater in women as compared with men (HR: 1.94, 95% CI 1.1-3.48; P=0.02).Conclusions The patients in this study population respond to treatment in less time and in comparison with other studies, their proteinuria recovers earlier. Class of lupus nephritis (negative) and gender (positive) were predictive factors proteinuria recovery among LN patients.
Synergism between nonane and emanations from soil as cues in oviposition-site selection of natural populations of Anopheles gambiae and Culex quinquefasciatus
Authors: Mwingira Victor S. , Mboera Leonard EG , Takken Willem
Published: March 2020

Background: Olfactory cues have been shown to have an important role in guiding gravid mosquito females to selected sites for egg laying. The objective of this study was to determine the influence of emanations from soil from a breeding site and the putative oviposition pheromone nonane on oviposition-site selection of natural populations of Anopheles gambiae s.l. and Culex quinquefasciatus.Methods: This field-based study was conducted in Mvomero District in East-central Tanzania. In a dual-choice experimental set up, clay bowls were dug into the ground and filled with one of the following treatments: (i) distilled water + autoclaved soil (control), (ii) distilled water + soil from a natural mosquito breeding site, (iii) distilled water + nonane and (iv) distilled water + nonane + soil from a natural breeding site. Soil was either left untreated or was autoclaved before use. The number of larvae present in the bowls after 10 d was used as outcome measure.Results: Autoclaved soil had a significant effect on oviposition behaviour of Cx. quinquefasciatus (P<0.005) but no effect on An. gambiae (P= 0.18). Nonane and emanations from untreated soil significantly influenced the selection of oviposition sites by both An. gambiae (P< 0.0001) and Cx. quinquefasciatus (P<0.0001). A mixture of nonane and untreated soil caused a synergistic effect on oviposition behaviour compared to either nonane or untreated soil alone, in both An. gambiae (Oviposition Activity Index (OAI) = 0.56; P< 0.001) and Cx. quinquefasciatus (OAI =0.59; P<0.0001).Conclusion: The larval pheromone nonane and emanations from breeding-site soil both induced oviposition in wild An. gambiae s.l. and Cx. quinquefasciatus, with a synergistic effect when both stimuli were present simultaneously. This is the first study in which nonane is shown to cause oviposition under natural conditions, suggesting that this compound can potentially be exploited for the management of mosquito vectors.
Scleroderma-specific autoantibodies embedded in immune complexes mediate endothelial damage: an early event in the pathogenesis of systemic sclerosis
Authors: Raschi Elena , Privitera Daniela , Bodio Caterina , Lonati Paola Adele , Borghi Maria Orietta , Ingegnoli Francesca , Meroni Pier Luigi , Chighizola Cecilia Beatrice
Published: March 2020

Background: Consistently with their diagnostic and prognostic value, autoantibodies specific for systemic sclerosis (SSc) embedded in immune complexes (ICs) elicited a pro-inflammatory and pro-fibrotic cascade in healthy skin fibroblasts, engaging Toll-like Receptors (TLRs) via their nucleic acid components. The objective of this study was to investigate the pathogenicity of SSc-ICs in endothelial cells (ECs).Methods: ICs were purified from sera of SSc patients bearing different autoantibody specificities (antibodies against DNA topoisomerase I, centromeric proteins, RNA polymerase and Th/To), patients with systemic lupus erythematosus (SLE), primary anti-phospholipid syndrome (PAPS) or healthy controls (NHS) using polyethylen glycol precipitation. Human umbilical vein ECs (HUVECs) were incubated with ICs, IL-1β, Poly I:C, LPS or ODN CpG. mRNA levels of endothelin-1 (ET-1), collagenIα1 (colIα1), interferon (IFN)-α and IFN-β were investigated by Real-Time PCR; ICAM-1 expression was evaluated by cell-ELISA; secretion of IL-6, IL-8 and tumour growth factor (TGF)-β1 in culture supernatants was measured by ELISA. Intracellular signalling pathways culminating with NFkB, p38MAPK, SAPK-JNK and Akt were assessed by Western Blotting. Healthy skin fibroblasts were stimulated with supernatants from HUVEC incubated with ICs, and tumour growth factor (TGF)-β1secretion, mRNA levels of colIα1 and matrix metalloproteinase (mmp)-1 were evaluated.Results: All SSc stimulated IL-6 secretion compared to NHS-ICs; ACA-ICs and anti-Th/To-ICs increased ICAM-1 expression; all SSc-ICs but anti-Th/To-ICs augmented IL-8 levels; all SSc-ICs but ACA and ARA-ICs up-regulated et-1 and all SSc-ICs but ARA-ICs affected TGF-β1 secretion. ColIα1, IFN-α and IFN-β mRNA levels were not affected by any SSc-ICs. A differential modulation of tlr expression was observed: tlr2, tlr3 and tlr4 were upregulated by ATA-ICs and ACA-ICs, while anti-Th/To-ICs resulted in tlr9 up-regulation. All SSc-ICs activated p38MAPK and AKT, all SSc-ICs but ARA-ICs yielded the activation of NFκB; ATA-ICs and ACA-ICs increased the activation rate of both subunits of SAPK-JNK.When healthy skin fibroblasts were stimulated with supernatants from HUVECs incubated with SSc-ICs, TGF-β1 secretion and colIα1 expression levels were significantly modulated.Conclusions: These data provide the first demonstration of the pathogenicity of ICs from scleroderma patients with different autoantibodies in ECs. Endothelial activation induced by SSc-ICs ultimately led to a pro-fibrotic phenotype in healthy skin fibroblasts.
Risk factors for lymph node metastasis in gastric neuroendocrine tumor: a retrospective study
Authors: Li Xianghui , Wang Meng , Lu Xiaofeng , Yang Zhengyang , AI Shichao , Sun Feng , Guan Wenxian , Liu Song
Published: March 2020

Background: Lymph node metastasis (LNM) plays a vital role in the determination of clinical outcome in patients with gastric neuroendocrine tumor (G-NET). Preoperative identification of LNM is helpful for intraoperative lymphadenectomy. This study aims to investigate risk factors for LNM in patients with G-NET. Method: We performed a retrospective study involving 37 patients in non-LNM group and 82 patients in LNM group. Data of demographics, preoperative lab result, clinical pathological results, surgical management and postoperative situation were compared between groups. Significant parameters were subsequently entered into logistic regression for further analysis. Results: Patients in LNM group exhibited older age (p=0.011), lower preoperative albumin (ALB) (p=0.003), higher carcinoembryonic antigen (CEA) (p=0.020), higher International normalized ratio (p=0.034), longer thrombin time (p=0.018), different tumor location (p=0.005), higher chromogranin A positive rate (p=0.045), and higher Ki-67 expression level (p=0.002). Logistic regression revealed ALB (p=0.043), CEA (p=0.032), tumor location (p=0.013) and Ki-67 (p=0.041) were independent risk factors for LNM in G-NET patients. Conclusions: ALB, CEA, tumor location and Ki-67 expression level correlate with the risk of LNM in patients with G-NET.
The esophagus survives non-thermal irreversible electroporation ablation and gradually rehabilitates
Authors: Song Yue , Zheng Jingjing
Published: March 2020

Background When using ablation technology to treat esophageal cancer or tumors adjacent to the esophagus, the damage and recovery of the esophagus itself is of particular concern. Non-thermal irreversible electroporation (NTIRE) is a novel minimally invasive ablation method that uses microsecond electric fields to create nanoscale defects on cell membranes and induce cell death, while keeping all other molecules (including the extracellular matrix) intact. In this study, we aimed to study the effect of NTIRE on the esophagus wall and its subsequent repair process.Methods A typical NTIRE electrical protocol was applied to the rabbit esophagus and histological analysis was used to analyze subsequent changes in esophageal tissue after ablation.Results The application of NTIRE resulted in complete cell inactivation, but the experimental animals did not show severe pathological discomfort after in situ ablation. After ablation, the entire layer of the esophageal wall gradually showed signs of recovery: regeneration and creep replacement of epithelial basal cells, regeneration of muscle cells and structural remodeling of the muscle layer, and finally the restoration of clear anatomical structures in each layer.Conclusions The esophagus can survive the direct application of NTIRE, and the whole layer of the entire wall can be gradually regenerated and repaired. NTIRE can be used for in situ ablation of local tumors, including esophageal, intrathoracic and mediastinal tumors, and upper abdominal tumors, while minimizing collateral damage to adjacent tissues because of the unique ability of the NTIRE ablation method to target the cell membrane.
Ectopic expression of Cripto-1 in transgenic mouse liver promotes hepatocyte proliferation, and causes the altered expression of hepatocarcinogenesis-related genes
Authors: Liu Yu , Li Yan-Qing , Jia Jun-Shuang , Zhao Wen-Tao , Wei Fang , Wang Jia-Hong , Dai Guan-Qi , Huang Shi-Hao , Wang Yu-Cai , Li Yong-Long , Li Xiao-Yan , Han Liu-Xin , Xiao Dong , Xu Kang , Lin Xiaolin
Published: March 2020

Background : Embryonic gene Cripto-1 (CR-1) is not detected or expressed at low levels in normal adult tissues, however, is re-expressed in numerous tumors, including hepatocellular carcinoma (HCC) and CR-1 transgene overexpression led to mammary epithelial hyperplasia and adenocarcinoma, which prompted us to investigate whether hepatocyte-specific overexpression of CR-1 in transgenic mice can initiate hepatocarcinogenesis. Methods : Semi-quantitative RT-PCR, qRT-PCR, Western blotting or immunohistochemical staining were employed to detected CR-1 mRNA transcript or CR-1 expression in HCC tissue specimens. Combined use of CR-1 transgenic mice, microarray, 2/3 partial hepatectomy (PHx), and TCGA and GEO database examined the functions of CR-1 in initiate hepatocelluar carcinogenesis. Results: Here we firstly provided the direct evidence that a truncated 1.7-kb mRNA transcript from CR-1 geneis dominantly expressed in HCC specimens, suggesting the importance of short CR-1 mRNA form in HCC progression.We found that CR-1 is frequently upregulated in HCC specimens. Hepatocyte-specific overexpression of CR-1 in transgenic mice promoted hepatocyte proliferation after 2/3 PHx, CR-1 positively regulated HCC cell proliferation and invasion in vitro, and CR-1 overexpression promoted tumour growth of HCC cells in nude mice. In this study, we demonstrated that CR-1 transgenic overexpression in transgenic liver abnormally activated downstream pathways (i.e., AKT, Wnt/b-catenin, Stat3, MAPK/ERK, JNK, TGF-b and Notch) and led to the deregulated expression profile of genes, including up-regulated genes (i.e., CD5L, S100A8, S100A9, Timd4, Orm2, Orm3, Saa1, Saa3, Itih3, Itih4, Ly6e, IGHG1, IGHG2B and Vnn3) and down-regulated genes (i.e.,PDK4, DMBT1, G0S2, Plk2, Plk3, Gsta1 and Gsta2), all of which are key regulators of cellular proliferation, inflammation response, cellular malignant transformation, hepatocarcinogenesis or HCC progression, however, there were no histological signs of precancerous lesions, hepatocyte dysplasia and HCC formation in livers samples from 3-, 6- or 8-month-old RCLG/Alb-Cre transgenic mice, suggesting that the constitutive expression of CR-1 alone is not sufficient to promote hepatocarcinogenesis, unless other factors, i.e., a second hit, is present. Conclusions: Taken together, we provide the first in vivo genetic evidence that CR-1 overexpression in transgenic mouse liver promotes hepatocyte proliferation after 2/3 PHx, and causes the deregulated molecular alterations involved in HCC oncogenesis, however, these abnormally molecular alterations are not sufficient to initiate hepatocarcinogenesis in mice. Keywords : Cripto-1 (CR-1), hepatocellular carcinoma (HCC), transgenic mice, hepatocarcinogenesis
Assessment of Biventricular Function in Clinically Well Pediatric Heart Transplantation Patients by Three-dimensional Speckle Tracking Echocardiography
Authors: Lv Qing , Li Meng , Li He , Wu Chun , Dong Nianguo , Li Yuman , Zhang Li , Xie Mingxing
Published: March 2020

Background Studies on pediatric heart transplantation (HTx) are uniquely challenging because pediatric HTx center volumes are generally low. And, the biventricular function plays an important role in the prognosis of pediatric HTx. The primary aim of our study was to evaluate biventricular function of pediatric HTx by three-dimensional speckle tracking echocardiography(3D-STE). Methods We enrolled 30 clinically well pediatric HTx patients and 30 sex- and age- matched healthy controls. All subjects underwent comprehensive echocardiographic examinations. Left ventricular (LV) global longitudinal strain (GLS), global circumferential strain (GCS), LV and right ventricular (RV) ejection fraction (EF) and RV longitudinal strain (RVLS) of free wall and septum were acquired by 3D-STE. And the correlations between strains and clinical data were explored. Results Compared with controls, LV GLS was decreased in pediatric HTx patients (P<0.05), while LV GCS and LVEF showed no difference. RVEF, RVLS (free wall) and RVLS (septum) in HTx group were diminished (P<0.05), but RVEF was still in normal range. Cold ischemic time was correlated inversely with LV GLS (β=-0.401, P<0.05). The mean pulmonary artery pressure (β=0.447, P<0.05) and postoperative tricuspid regurgitation pressure (β=0.607, P<0.05) were associated with RVLS (free wall). Conclusion Biventricular longitudinal systolic function rather than global systolic function was impaired after HTx. 3D STE may be able to evaluate the ventricular function better. Prolonged ischemic time leads to impaired LV longitudinal systolic function in pediatric HTx patients. It’s interesting that in HTx patients, it shows compensatory enhancement due to increased pulmonary vascular resistance.
Screening Cabbage Cultivars for Resistance to Black Rot under Field Conditions
Authors: Ribeiro da Silva Andre Luiz Biscaia , Candian Joara Secchi , do Rego Elizanilda Ramalho , Coolong Timothy , Dutta Bhabesh
Published: March 2020

The use of resistant cultivars against Xanthomonas campestris pv. campestris (Xcc) is considered a critical management practice for black rot (BR) management in cabbage (Brassica oleracea var. capitata). Although most studies that have evaluated resistance to BR were conducted in greenhouses without accounting for yield, there is a clear need to investigate cultivar performance under field conditions. The objectives of this study were to evaluate commercial cabbage cultivars for resistance to BR and determine yield and head quality under field conditions. Field experiments with eight cultivars (Acclaim, Bravo, Capture, Celebrate, Cheers, Melissa, Monterey, and TCA-549) were conducted in two cabbage growing seasons, Fall 2018 and Spring 2019. Fields were spray-inoculated with Xcc (3 × 105 cfu/mL) at 35 and 49 days after transplanting in Fall 2018 and Spring 2019, respectively. Cabbage BR severity was evaluated at weekly intervals starting from 7 days postinoculation (DPI) until harvest. Marketable and unmarketable yields and cabbage head quality were measured at harvest. Cabbage BR symptoms were detected in all tested cultivars for both growing seasons with initial symptoms observed as early as 28 and 21 DPI in Fall 2018 and Spring 2019, respectively. Severity of cabbage BR at harvest was significantly greater in Fall 2018 compared with Spring 2019, whereas marketable yield was significantly higher in Spring 2019 (45,169 lb/acre) compared with Fall 2018 (26,370 lb/acre). In both growing seasons, ‘TCA-549’ had the lowest severity of BR and ‘Melissa’ had the highest severity of BR. Area under the disease progress curve (AUDPC) was 175 and 13 for ‘TCA-549’ in Fall 2018 and Spring 2019, respectively. The AUDPC for ‘Melissa’ in Fall 2018 and Spring 2019 were 2376 and 905, respectively. Regardless of growing season, cabbage marketable yield was higher for ‘Acclaim’ (51,760 lb/acre) compared with all other cultivars; however, there was no significant difference between Acclaim and TCA-549 (42,934 lb/acre) for cabbage marketable yield. Cabbage marketable yield was the lowest for cultivars Melissa (18,275 lb/acre) and Capture (24,236 lb/acre). Overall, there was a significant correlation between BR disease severity and cabbage marketable and unmarketable yields. Increasing the BR severity decreased cabbage marketability due to an increase in unmarketable yields. Continued development of cultivar resistance to BR is important for cabbage production in the southeastern United States, given the favorable conditions for disease development. The use of cultivars with low susceptibility, pathogen-free seeds, crop rotation, and proper spraying programs should be considered the best management practice for BR disease.
A integralidade no trabalho em grupo em saúde mental
Authors: De Almeida Jeanie da Silva Barreiro , Batista Rafaela Das Mercês , Do Prado Thiago Nascimento
Published: March 2020

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A importância do papel do farmacêutico na Atenção Básica
Authors: Dos Santos Vitor Barbosa , Da Rosa Priscila Santos , Leite Franciele Marabotti Costa
Published: March 2020

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Ações de controle da tuberculose na atenção primária brasileira
Authors: Barbosa Bruna Lígia Ferreira de Almeida , Lima Eliane de Fátima Almeida , Orlandi Elissa , Lima Rita de Cássia Duarte
Published: March 2020

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Editorial
Authors: Miotto Maria Helena Monteiro de Barros
Published: March 2020

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O Facebook faz bem à saúde? O caso “MAIS PARTICIPAÇÃO melhor saúde” em Portugal
Authors: Crisóstomo Sofia , Matos Ana Raquel , Borges Marta , Santos Margarida
Published: March 2020

Introdução: Ao longo da última década, assistimos ao recrudescimento de novas formas de ação coletiva, como consequência do forte impulso que as tecnologias de comunicação vieram dar às mobilizações coletivas. Objetivo: Apresentar o projeto de investigação-ação “MAIS PARTICIPAÇÃO melhor saúde” (MPms) – um movimento social na área da saúde – e demonstrar de que forma a utilização que este fez da internet o tornou um caso paradigmático de ativismo virtual em saúde. Métodos: Partindo da caracterização do projeto MPms e da sua dinâmica participativa e em rede, analisa-se de que forma o recurso preferencial à internet e às redes sociais – em particular, o Facebook - e ao contato online, funcionou como elemento transformador da realidade da participação na área da saúde em Portugal. Resultados: O MPms procurou, desde o início, alimentar uma forte presença virtual, consolidando uma base social de apoio em torno dos seus objetivos, divulgando ideias e iniciativas e lançando estrategicamente debates e questões ao seu público-alvo. Esse ativismo virtual veio contribuir de forma indelével para, por um lado, a sensibilização para a importância pública dos cidadãos participarem na decisão em saúde e, por outro, para o reforço do envolvimento regular dos cidadãos nas discussões em saúde, procurando uma capacitação que lhes permita uma futura participação em saúde verdadeiramente transformadora. Conclusão: Os resultados mostram que o net-ativismo constitui uma alavanca fundamental para a mobilização, ação coletiva e sensibilização pública, na área da saúde.
Tendência temporal de prescrição de terapias biológicas para artrite reumatoide
Authors: Poliane Barbosa Sampaio Buffon  , Valéria Valim  , Bruna Costa  , Gabrieli Fernandes Freitas  , Eliana Zandonade
Published: March 2020

Objetivo: Analisar a tendência temporal de uso das terapias biológicas e descrever o perfil epidemiológico dos pacientes diagnosticados com Artrite Reumatoide (AR) em tratamento atendidos nas Farmácias Cidadãs do Espírito Santo (ES) pelo SUS. Métodos: Estudo de série temporal, realizado no ES, através de dados secundários sobre pacientes com AR em uso de terapia biológica nas 10 Farmácias Cidadãs do Estado, no período de 2009 a 2017. Para comparar as incidências anuais de AR, por Farmácia Cidadã, fez-se uma análise de tendência linear dessas séries temporais. Resultados: Houve 9.804 solicitações para o tratamento com as terapias biológicas, com dispensação concentradas nas Farmácias Cidadãs da Região Metropolitana do Estado (69%); com predominância de mulheres (84%), e idade média de 56 anos; o medicamento com maior prevalência foi o Adalimumabe (40%), seguido do Infliximabe (21%), com um custo total para o tratamento igual R$ 6.335.100,71. Conclusão: Essa análise evidencia o aumento expressivo na dispensação desses medicamentos em relação ao crescimento da incidência dos pacientes novos pelo SUS. O crescimento da demanda por esses medicamentos acarreta também um custo elevado para o SUS, uma preocupação com o futuro assumindo a premissa de que tais valores não devem reduzir nos próximos anos.
Síndrome de Cornélia e Lange: dificuldade encontradas quanto ao tratamento odontológico
Authors: Castilho Juliana Bosco , Piva Graziela Angeli , Guirardo Cecília Gatti
Published: March 2020

Síndrome de Cornélia de Lange; Etiologia; Dificuldades no manejo.
Dentes unirradiculados portadores de necrose pulpar e periapicopatias crônicas tratados endodonticamente em sessão única
Authors: Gonçalves Larisse Barbosa , Ribeiro Francisco Carlos , Roldi Armelindo , Pereira Rosana de Souza , Intra João Batista Gagno
Published: March 2020

Periapicopatias; Lesões periapicais; Dentes unirradiculados; Terapia endodôntica.
Regeneração Tecidual Guiada em defeitos de furca classe II
Authors: Chiarelli Fabio Matos , Reichardt Max , Feitosa Alfredo Carlos Rodrigues , Ganhoto Maria Rebeca Amaral , Bourguignon Filho Aguimar de Matos , Guimarães Luciano Bastos , Chiarelli Fabíola Gonçalves D. B.
Published: March 2020

Bolsas periodontais; Cirurgia periodontal; Terapia para furca; Regeneração Tecidual Guiada.
Aspectos oclusais nas lesões cervicais não cariosas
Authors: Lozer André Carlesso , Resende Paula Lopes de , Batitucci Eduardo , Batitucci Maria Hermegilda G.
Published: March 2020

Abfração; Estresse oclusal; Lesão cervical.
O envolvimento do cirurgião-dentista no preparo para braquiterapia
Authors: Barros Liliana Aparecida Pimenta de , Fracalossi Ana Carolina Cuzzuol , Lyrio Mariana Camilo Negreiros , Souza Letícia Nogueira da Gama
Published: March 2020

Carcinoma espinocelular; Palato; Braquiterapia.
Odontoma composto: relato de caso
Authors: Ferreira Lúcia de Fátima Paixão Emery , Campos Denise Maria Kroeff de Souza , Zeferino José Luiz Tarsia , Bautz Willian Grassi
Published: March 2020

Odontoma composto; Tumor odontogênico; Dentes impactados.
Análise sa concentração de fluoreto presente em enxaguatórios bucais confeccionados em farmácias de manipulação
Authors: Bastos Luciana Freitas , Monte Alto Luciane , Medeiros Urubatan , Reis Rodrigo
Published: March 2020

Flúor; Anti-sépticos bucais; Farmácias.
Tratamento da maloclusão esquelética de classe II na fase de pré-surto puberal com o aparelhoo funcional Twin Block - relato de um caso
Authors: Brunharo Ione Helena Vieira Portella , Quintão Cátia Cardoso Abdo , Cunha Deise Lima
Published: March 2020

Maloclusão; Classe II de Angle; Ortodontia preventiva; Pré-puberdade.
High-density Genetic Linkage Map Construction in Sunflower (Helianthus annuus L.) Using SNP and SSR Markers
Authors: Lyu Pin , Hou Jianhua , Yu Haifeng , Shi Huimin
Published: March 2020

Background:

Sunflower (Helianthus annuus L.) is an important oil crop only after
soybean, canola and peanuts. A high-quality genetic map is the foundation of marker-assisted
selection (MAS). However, for this species, the high-density maps have been reported limitedly.

Objective:

In this study, we proposed the construction of a high-density genetic linkage map by the
F7 population of sunflowers using SNP and SSR Markers.

Methods:

The SLAF-seq strategy was employed to further develop SNP markers with SSR
markers to construct the high-density genetic map by the HighMap software.

Results:

A total of 1,138 million paired-end reads (226Gb) were obtained and 518,900 SLAFs
were detected. Of the polymorphic SLAFs, 2,472,245 SNPs were developed and finally, 5,700
SNPs were found to be ideal to construct a genetic map after filtering. The final high-density
genetic map included 4,912 SNP and 93 SSR markers distributed in 17 linkage groups (LGs) and
covered 2,425.05 cM with an average marker interval of 0.49 cM.

Conclusion:

The final result demonstrated that the SLAF-seq strategy is suitable for SNP markers
detection. The genetic map reported in this study can be considered as one of the most highdensity
genetic linkage maps of sunflower and could lay a foundation for quantitative trait loci
(QTLs) fine mapping or map-based gene cloning.
Introdução
Authors: Dias Idalete , Araújo Sílvia , Sanromán Álvaro Iriarte , Williams Geoffrey
Published: March 2020

O presente número da revista Diacrítica é dedicado aos Corpora nas Humanidades Digitais. Na última década, temos assistido a grandes avanços no processamento de dados, motivados pelo estudo e pela análise da imensurável quantidade de informação que se encontra dispersa e cuja produção aumenta de dia para dia. Graças às novas tecnologias e ao desenvolvimentoda Ciência da Computação, a Linguística de Corpus abriu caminho ao estudo dos fenómenos linguísticos de forma nunca antes concebida, assim como a outras utilizações desenvolvidas à medida de diversos profissionais (professores, tradutores, linguistas, lexicógrafos, terminólogos, informáticos, historiadores, etc.). No âmbito deste volume, pretende-se dar uma visão das diferentes aplicações da Linguística de Corpus, com especial foco na investigação da linguagem em contexto académico.
Características identificadoras e dificuldades na aplicação de listas para a anotação de Entidades Geográficas Mencionadas
Authors: Canosa Afonso Xavier
Published: March 2020

Na anotação automática de entidades geográficas mencionadas, as listas especializadas de topónimos têm que enfrentar ambiguidades e contextos em que o valor geográfico de uma expressão não é evidente. Neste artigo, estuda-se o caso prático de um índice de topónimos utilizado para criar um corpus anotado da Peregrinação de Mendes Pinto. As dificuldades achadas servem para classificar os tipos de erros que se produzem quando o topónimo é resolvido pela simples coincidência de expressões e introduzem critérios para a identificação das entidades geográficas, uma tarefa que deve preceder e tem um impacto direto nos resultados obtidos no processo de anotação automática.
Uma versão em Português Europeu do C-test
Authors: Yamada Masayuki
Published: March 2020

A avaliação da proficiência é uma questão importante na interpretação dos resultados das investigações sobre a aprendizagem de língua segunda. Dados contraditórios entre os estudos podem decorrer dos diferentes procedimentos empregues para avaliar os aprendentes. De modo geral, são consideradas informações institucionais, estimativas baseadas no perfil linguístico e, mais raramente, são utilizados resultados de testes independentes. No presente estudo, foi desenvolvida uma versão portuguesa do C-test, um teste de preenchimento simples utilizado para medira proficiência geral. Elevados coeficientes de Cronbach Alfa e Omega revelaram a fiabilidade do teste. A classificação da proficiência dos aprendentes baseada na pontuação do teste demonstrou uma correlação forte com a avaliação pelos professores (r = 0.74), mostrando que o teste é eficiente para avaliar a proficiência geral. Para além disso, outros parâmetros, nomeadamente nível da turma e autoavaliação dos aprendentes também mostraram uma correlação forte com a avaliação por professores (r = 0.79 e 0.77). A utilização futura do teste é discutida.
Análise diacrónica dos tempos compostos "tinha feito", "terei feito" e "teria feito" na língua portuguesa
Authors: Hricsina Jan
Published: March 2020

Este artigo foca a análise diacrónica dos tempos compostos tinha feito, terei feito e teria feito na língua portuguesa. O seu objetivo principal é analisar as funções modo-temporais dos tempos compostos em questão no Português Antigo e comparar a sua frequência e o seu uso na evolução da língua portuguesa. A pesquisa tem por base o corpus linguístico www.corpusdoportugues.org.
The role of pragmatic markers in academic spoken interlanguage. A corpus-based study of a group of Brazilian EFL university students
Authors: Orfanò Bárbara Malveir , Oliveira Ana Larissa Adorno Marciotto , Da Silva Spencer Barbosa
Published: March 2020

The present work addresses a group of university students of EFL (English as a Foreign Language) on how they use pragmatic markers in their oral productions.The initial hypothesis was that there would be differences both in usage and form in comparison to native speakers. In order to verify our claim, we set off to investigate two corpora: a learner oral corpus being compiled at the Federal University of Minas Gerais/Brazil and a sub-corpus from the British Academic Spoken English (BASE). While Brazilian students overuse items such as maybe and just, the datarecorded in the UK displayed a more varied range of markers and multiword forms. Overall, the findings reinforce the importance of analyzing empirical data for a broader understanding of how native speakers and learners can differ in their oral academic production. The paper also sheds light on language teaching and learning in the academic setting from a pragmatic viewpoint.
Corpus stylistics in translation-oriented text analysis. Approaching the work of Denton Welch from a functionalist perspective
Authors: Braga Guilherme da Silva
Published: March 2020

This article is an effort towards interpreting the findings of a corpus-based stylistic analysis of the short narrative “Sickert at St Peter’s” (1942), written by the English writer and painter Denton Welch (1915–1948), within the larger framework for translation-oriented text analysis presented by Christiane Nord in Textanalyse und Übersetzen (2009). The aim is to explore both the theoretical possibilities andthe practical applications of a corpus-based approach to the lexical analysis phase of Nord’s model from a literary translation perspective, in which style and word choice play a critical role. Once the statistical findings of the corpus-based analysis are presented, the 25 highest-ranking keywords in the text are analyzed in context. Translation briefs and literary translation in general are discussed, and a global pre-translational strategy for translating “Sickert at St Peter’s” is presented. By way of conclusion, it is argued that the method described promotes valuable insights for literary interpretation and serves as a practical aid in developing a pre-translational strategy for translating individual texts.
Em vida e na hora da morte também. O que dizem registros de óbito oitocentistas da Freguesia de Nossa Senhora da Penha de Corumbá (1847-1855)
Authors: De Paula Maria Helena , De Amorim Amanda Moreira
Published: March 2020

A história do Brasil está profundamente atrelada a um regime escravocrata, responsável pela importação de mão de obra africana escrava para o país, que vigor opor aproximadamente quatro séculos. Como ocorreu em grande parte do Brasil, o estado de Goiás teve destacado papel na história da escravidão africana neste país, a comprovarem diversos manuscritos de naturezas variadas, que mencionam escravos que viveram no local. Este artigo propõe analisar histórica e linguisticamente um livro de registro de óbitos, composto por documentos eclesiásticos exarados entre 1847 e 1855, sobre a Freguesia de Nossa Senhora da Penha de Corumbá, visando a estabelecer relações entre a expectativa de vida dos escravos, dos libertos, bem como dos livres, com base em dados como gênero e idade dos falecidos. Para realizar o proposto, elaboramos um inventário com informações referentes aos sujeitos descritos nos registros, baseando-nos no modelo proposto por Santose Paula (2014) e cotejamos os dados obtidos com estudos que abordam o período escravocrata brasileiro, como os de Libby e Paiva (2005), Paiva (2014), Salles (1992) e outros. Assentado em uma perspectiva de interface dos estudos de Filologia, Linguística e História, este estudo busca compreender aspectos históricos e culturais registrados nos documentos e que sobremaneira os caracterizam.
Focus. Sola Scriptura: een kwestie van gezag
Authors: van Spanje T.E.
Published: March 2020

N.a.v. Matthew Barrett, God’s Word Alone: The Authority of Scripture. What the Reformers Taught ... and Why It Still Matters (The Five Solas Series; Grand Rapids: Zondervan, 2016) 402 p., $ 28.99 (ISBN 9780310515722).
A Bidirectional Associations between Urban Physical Environment and Mental Health: A theoretical framework
Authors: Marzukhi Marlyana Azyyati , Ghazali Nur Masyitah , Leh Oliver Ling Hoon , Yakob Hamizah
Published: March 2020

This paper examines the influence of the urban physical environment on mental health. A scoping review of reviews and a search of keywords from 109 international literatures from 1985 to 2019 conducted in a relevant database such as SCOPUS, Web of Science, and PubMed and snowballing method in the references list to get a recent paper that related. The relationship developed in the form of a theoretical framework using urban health indicators that identifies a relationship between the urban physical environment and mental health. The framework can be beneficial to the urban planning decision-makers to plan healthier and more sustainable Malaysian cities.Keywords: Urban Physical; Environment; Mental; HealtheISSN: 2398-4287 © 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.DOI: https://doi.org/10.21834/e-bpj.v5i13.2048
Factors Influencing Active Travel Mode to School for Primary School Children
Authors: Osman Naimah , Nasrudin Na'asah , Abdullah Yusfida Ayu , Khalid Nurul Shakila
Published: March 2020

Walking is a healthy way for children to explore their environment. However, nowadays, children prefer a passive mode of transport to commute to school. This paper aims to identify the factors that influence children to walk to school and evaluates the safety level of the children. This study, therefore, investigates children's preference to understand their perceptions on the choice to walk to school involving 100 primary school children in the Shah Alam City, Malaysia, through a case study method. Results indicated that distance to school, quality of the pedestrian facility and surrounding environment in a facility area affect the decision of children to walk to school.Keywords: Active Travel Mode; Walking; Primary School ChildreneISSN: 2398-4287 © 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).  Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.DOI: https://doi.org/10.21834/e-bpj.v5i13.2089
Analysis of Sample Rejection and the Impact on Quality of Care in Patients in a Single Tertiary Healthcare Facility in Malaysia
Authors: Kamal Fatmawati , Wan Mohd Saman Wan Asmuni , Adbul Monir Madyhah
Published: March 2020

The Malaysian Society for Quality in Health (MSQH), recommended a rejection rate of less than 1%. Many studies reported rejection rates of 0.1% to 3.49%. This study was conducted at the UiTM Medical Specialist Centre from January 2019 to October 2019. The blood bank and haematology workstation both rejected 84 (5.7%) and 560 (3.38%) samples, respectively. The main causes of sample rejection were clotted and lysed samples. Most of the rejected samples came from the wards, followed by the emergency department. This study showed the need for improvement in venipuncture techniques and types of equipment.Keywords: Rejection rates; Sample rejection; Quality of care; Tertiary centreeISSN: 2398-4287 © 2020. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.DOI: https://doi.org/10.21834/e-bpj.v5i13.2104
Análise volumétrica de adenoma pleomórfico palatino como aprimoramento diagnóstico e de plano de tratamento: relato de caso
Authors: Pontes Castro Fernando Antônio , Da Silva Firmino Brunna , Araujo de Sousa André Luca , Da Silva Araujo José Duylles , Perez Gomes João Pedro , Ferreira Costa André Luiz , Pereira de Castro Lopes Sérgio Lucio , De Pinho Mendes Jean , Santos Bezerra Pinto Antonione
Published: March 2020

O adenoma pleomórfico (AP) é o tumor benigno mais comum dentre as neoplasias das glândulas salivares. Softwares de reconstrução tridimensional oferecem ferramentas de caracterização da lesão, permitindo avaliar a morfologia da doença, analisar estruturas anatômicas isoladas, e elevar a acurácia do tratamento. O objetivo deste artigo é demonstrar como o uso da reconstrução tridimensional tem o potencial de contribuir para a elucidação da morfologia da doença, levando os profissionais da saúde à execução de um tratamento preciso e eficaz por meio de diagnóstico e planejamentos pré- operatórios virtuais. Apresenta-se um caso de um homem de 37 anos, portador de adenoma pleomófico palatino sem queixa álgica. Não apenas a morfologia da doença foi estudada, mas o volume da lesão foi calculado, medindo 2.304 mm3. O volume da doença atuou como um marcador que possibilitou avaliar a extensão da lesão e o comprometimento das regiões adjacentes.