bluuebunny/crossref_metadata_2025 huggingface dataset:
A Paradoxical Role for Regulatory T Cells in the Tumor Microenvironment of Pancreatic Cancer
Authors: Brouwer Thomas , Ijsselsteijn Marieke , Oosting Jan , Ruano Dina , van der Ploeg Manon , Dijk Frederike , Bonsing Bert , Fariña Arantza , Morreau Hans , Vahrmeijer Alexander , Miranda Noel de
Published: August 2022

Pancreatic ductal adenocarcinoma (PDAC) is considered to be a poorly immunogenic cancer type that combines a low mutation burden with a strong immunosuppressive tumor microenvironment. Regulatory T cells (Tregs) are major drivers of immune suppression but their prognostic role, particularly in gastrointestinal malignancies, remains controversial. Lymphocytic infiltration in 122 PDAC samples was assessed by multispectral immunofluorescence with anti-Keratin, -CD3, -CD8, -FOXP3 and -CD163 antibodies. Differential infiltration by Tregs was analyzed in the context of transcriptomic profiles that were available for 65 tumors. High infiltration of CD3+CD8− (mainly CD4+) T cells and, especially, of the subset expressing FOXP3 (Tregs) was associated with improved patient survival, whilst cytotoxic CD3+CD8+ T cell infiltration did not have an impact on overall survival. Transcriptomic analysis revealed three signatures in PDAC tumors comprising of epithelial-mesenchymal transition (EMT)/stromal, metabolic, and secretory/pancreatic signature. However, none of these signatures explained differences in Treg infiltration. We show that Tregs associate with improved overall survival in PDAC patients. This effect was independent of cytotoxic T cell infiltration and the transcriptomic profiles of their respective tumors. These findings provide a new layer of complexity in the study of PDAC tumor microenvironment that must be considered when developing immunotherapeutic interventions for this disease.
Iminosugar-Phosphines as Organocatalysts in the [3 + 2] Cycloaddition of Allenoates and N-Tosylimines
Authors: Elías-Rodríguez Pilar , Carmona Ana T. , Moreno-Vargas Antonio J. , Robina Inmaculada
Published: August 2022

Iminosugar derivatives containing a pyrrolidine-phosphine moiety were prepared from carbohydrates and used as catalysts in the [3 + 2] cycloaddition reaction between alkyl allenoates and electron-deficient imines. The corresponding 1,2,3,5-tetrasubstituted pyrrolines were obtained in good yields and diastereoselectivities but with moderate enantiocontrol. The stereochemical outcome of the reaction depends on the substituent at the nitrogen atom and hydroxyl groups, the configuration of the stereogenic centers and the distance between the diphenylphosphine group and the pyrrolidine skeleton of the catalyst. The preparation of both enantiomers of the catalyst allowed the corresponding enantiomeric pyrrolines to be obtained with similar yields, diastereo- and enantioselectivities.
Urolithins Modulate the Viability, Autophagy, Apoptosis, and Nephrin Turnover in Podocytes Exposed to High Glucose
Authors: Kotewicz Milena , Krauze-Baranowska Mirosława , Daca Agnieszka , Płoska Agata , Godlewska Sylwia , Kalinowski Leszek , Lewko Barbara
Published: August 2022

Urolithins are bioactive compounds generated in human and animal intestines because of the bacterial metabolism of dietary ellagitannins (and their constituent, ellagic acid). Due to their multidirectional effects, including anti-inflammatory, antioxidant, anti-cancer, neuroprotective, and antiglycative properties, urolithins are potential novel therapeutic agents. In this study, while considering the future possibility of using urolithins to improve podocyte function in diabetes, we assessed the results of exposing mouse podocytes cultured in normal (NG, 5.5 mM) and high (HG, 25 mM) glucose concentrations to urolithin A (UA) and urolithin B (UB). Podocytes metabolized UA to form glucuronides in a time-dependent manner; however, in HG conditions, the metabolism was lower than in NG conditions. In HG milieu, UA improved podocyte viability more efficiently than UB and reduced the reactive oxygen species level. Both types of urolithins showed cytotoxic activity at high (100 µM) concentration. The UA upregulated total and surface nephrin expression, which was paralleled by enhanced nephrin internalization. Regulation of nephrin turnover was independent of ambient glucose concentration. We conclude that UA affects podocytes in different metabolic and functional aspects. With respect to its pro-survival effects in HG-induced toxicity, UA could be considered as a potent therapeutic candidate against diabetic podocytopathy.
Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy
Authors: Huang Zhiying , Gan Shu , Zhuang Xuerong , Chen Yao , Lu Linlin , Wang Ying , Qi Xiaoxiao , Feng Qian , Huang Qiuju , Du Biaoyan , Zhang Rong , Liu Zhongqiu
Published: August 2022

Although artesunate has been reported to be a promising candidate for colorectal cancer (CRC) treatment, the underlying mechanisms and molecular targets of artesunate are yet to be explored. Here, we report that artesunate acts as a senescence and autophagy inducer to exert its inhibitory effect on CRC in a reactive oxygen species (ROS)-dependent manner. In SW480 and HCT116 cells, artesunate treatment led to mitochondrial dysfunction, drastically promoted mitochondrial ROS generation, and consequently inhibited cell proliferation by causing cell cycle arrest at G0/G1 phase as well as subsequent p16- and p21-mediated cell senescence. Senescent cells underwent endoplasmic reticulum stress (ERS), and the unfolded protein response (UPR) was activated via IRE1α signaling, with upregulated BIP, IRE1α, phosphorylated IRE1α (p-IRE1α), CHOP, and DR5. Further experiments revealed that autophagy was induced by artesunate treatment due to oxidative stress and ER stress. In contrast, N-Acetylcysteine (NAC, an ROS scavenger) and 3-Methyladenine (3-MA, an autophagy inhibitor) restored cell viability and attenuated autophagy in artesunate-treated cells. Furthermore, cellular free Ca2+ levels were increased and could be repressed by NAC, 3-MA, and GSK2350168 (an IRE1α inhibitor). In vivo, artesunate administration reduced the growth of CT26 cell-derived tumors in BALB/c mice. Ki67 and cyclin D1 expression was downregulated in tumor tissue, while p16, p21, p-IRE1α, and LC3B expression was upregulated. Taken together, artesunate induces senescence and autophagy to inhibit cell proliferation in colorectal cancer by promoting excessive ROS generation.
Growth State-Dependent Expression of Arachidonate Lipoxygenases in the Human Endothelial Cell Line EA.hy926
Authors: Sabbir Mohammad G. , Wigle Jeffrey T. , Taylor Carla G. , Zahradka Peter
Published: August 2022

Endothelial cells regulate vascular homeostasis through the secretion of various paracrine molecules, including bioactive lipids, but little is known regarding the enzymes responsible for generating these lipids under either physiological or pathophysiological conditions. Arachidonate lipoxygenase (ALOX) expression was therefore investigated in confluent and nonconfluent EA.h926 endothelial cells, which represent the normal quiescent and proliferative states, respectively. mRNAs for ALOX15, ALOX15B, and ALOXE3 were detected in EA.hy926 cells, with the highest levels present in confluent cells compared to nonconfluent cells. In contrast, ALOX5, ALOX12, and ALOX12B mRNAs were not detected. At the protein level, only ALOX15B and ALOXE3 were detected but only in confluent cells. ALOXE3 was also observed in confluent human umbilical artery endothelial cells (HUAEC), indicating that its expression, although previously unreported, may be a general feature of endothelial cells. Exposure to laminar flow further increased ALOXE3 levels in EA.hy926 cells and HUAECs. The evidence obtained in this study indicates that proliferative status and shear stress are both important factors that mediate endothelial ALOX gene expression. The presence of ALOX15B and ALOXE3 exclusively in quiescent human endothelial cells suggests their activity likely contributes to the maintenance of a healthy endothelium.
Neuro-Axonal Damage and Alteration of Blood–Brain Barrier Integrity in COVID-19 Patients
Authors: Zingaropoli Maria Antonella , Iannetta Marco , Piermatteo Lorenzo , Pasculli Patrizia , Latronico Tiziana , Mazzuti Laura , Campogiani Laura , Duca Leonardo , Ferraguti Giampiero , De Michele Manuela , Galardo Gioacchino , Pugliese Francesco , Antonelli Guido , Andreoni Massimo , Sarmati Loredana , Lichtner Miriam , Turriziani Ombretta , Ceccherini-Silberstein Francesca , Liuzzi Grazia Maria , Mastroianni Claudio Maria , Ciardi Maria Rosa
Published: August 2022

Neurofilament light chain (NfL) is a specific biomarker of neuro-axonal damage. Matrix metalloproteinases (MMPs) are zinc-dependent enzymes involved in blood–brain barrier (BBB) integrity. We explored neuro-axonal damage, alteration of BBB integrity and SARS-CoV-2 RNA presence in COVID-19 patients with severe neurological symptoms (neuro-COVID) as well as neuro-axonal damage in COVID-19 patients without severe neurological symptoms according to disease severity and after recovery, comparing the obtained findings with healthy donors (HD). Overall, COVID-19 patients (n = 55) showed higher plasma NfL levels compared to HD (n = 31) (p < 0.0001), especially those who developed ARDS (n = 28) (p = 0.0005). After recovery, plasma NfL levels were still higher in ARDS patients compared to HD (p = 0.0037). In neuro-COVID patients (n = 12), higher CSF and plasma NfL, and CSF MMP-2 levels in ARDS than non-ARDS group were observed (p = 0.0357, p = 0.0346 and p = 0.0303, respectively). SARS-CoV-2 RNA was detected in four CSF and two plasma samples. SARS-CoV-2 RNA detection was not associated to increased CSF NfL and MMP levels. During COVID-19, ARDS could be associated to CNS damage and alteration of BBB integrity in the absence of SARS-CoV-2 RNA detection in CSF or blood. CNS damage was still detectable after discharge in blood of COVID-19 patients who developed ARDS during hospitalization.
Correction: Kumari et al. A Long-Term Spatiotemporal Analysis of Vegetation Greenness over the Himalayan Region Using Google Earth Engine. Climate 2021, 9, 109
Authors: Kumari Nikul , Srivastava Ankur , Dumka Umesh Chandra
Published: August 2022

There was an error in the original publication [...]
Recent Advances of Preparation and Application of Two-Dimension van der Waals Heterostructure
Authors: Song Luhang , Song Moru , Lu Zongyu , Yu Gang , Liang Zhan , Hou Wei , Liao Qingwei , Song Yujun
Published: August 2022

With paramount electrical, optical, catalytic, and other physical and chemical properties, van der Waals heterostructures (vdWHs) have captured increasing attention. vdWHs are two-dimension (2D) heterostructures formed via van der Waals (vdW) force, paving the way for fabricating, understanding, and applications of 2D materials. vdWHs materials of large lattice constant difference can be fabricated together, forming a series of unique 2D materials that cannot form heterostructures earlier. Additionally, vdWHs provide a new platform to study the interlayer interactions between materials, unraveling new physics in the system. Notably, vdWHs embody short-range bonds weaker than covalent and ionic bonds, almost only interactions between nearest particles are considered. Owing to a clear interface, vdW interaction between two different components, devices made by vdWHs can bring amazing physicochemical properties, such as unconventional superconductivity, super capacitance in intercalation 2D structure, etc. Recently, impressive progress has been achieved in the controlled preparation of vdWHs and various applications, which will be summarized in this review. The preparation methods comprise mechanical exfoliation, liquid phase stripping, physical vapor deposition, chemical vapor deposition, and metalorganic chemical vapor deposition. The applications sections will focus on photoelectric devices, logic devices, flexible devices, and piezotronics. Finally, some perspectives in the future on the controlled preparation of vdWHs with desired properties for advanced applications will be discussed.
Light Hole Excitons in Strain-Coupled Bilayer Quantum Dots with Small Fine-Structure Splitting
Authors: Shang Xiangjun , Liu Hanqing , Su Xiangbin , Li Shulun , Hao Huiming , Dai Deyan , Chen Zesheng , Ni Haiqiao , Niu Zhichuan
Published: August 2022

In this work, we measure polarization-resolved photoluminescence spectra from excitonic complexes in tens of single InAs/GaAs quantum dots (QDs) at the telecom O-band with strain-coupled bilayer structure. QDs often show fine-structure splitting (FSS) ~100 μeV in uniform anisotropy and valence-band mixing of heavy holes (HH) and light holes (LH); the biaxial strain also induces LH excitons with small FSS (especially XX, <5 μeV, 70% of QDs); delocalized LH reduces the Coulomb interaction between holes Vhh and enhances population on LH excitons XX, XX11, X11+ and XX21+.
The Effect of Different Copper Discs on the Discharge of Superconducting Coils
Authors: Xia Yajun , Song Yuntao , Liu Huajun , Lu Zhen , Zheng Jinxing , Liu Fang , Song Meng
Published: August 2022

High temperature superconducting (HTS) magnets often work at high energy density and have slow quench propagation speed, so a quench will present a serious risk to the safety of magnets. The quench protection method based on the dump resistance can effectively reduce the current and release the energy in the HTS magnets. However, too large dump resistance may cause excessive voltage across the magnets. A quench protection system consisting of dump resistances and metal discs has been proposed. Copper discs are often embedded in HTS magnets for conducting cooling and mechanical support. In the discharging process of HTS magnets, the copper discs can absorb energy from the magnets through magnetic coupling, thus accelerating the current decay of the magnets. This quench protection method is more effective than using dump resistance alone. In this paper, the effect of different copper discs on the discharging process of HTS coils is discussed. Eight types of copper with different residual resistivity ratios (RRR) are applied. The results show that with the increase of the RRR of the copper disc, the current decay rate of the coil increases, and the energy absorbed by the copper disc from the coil increases. The role of different copper discs in the fast quench protection of the coil can be sorted as: RRR = 300 > RRR = 100 > RRR = 80 > RRR = 60 > RRR = 40 > RRR = 30 > RRR = 20 > RRR = 10. The copper disc with RRR of 300 shows the best performance in quench protection of HTS coils.
DNA Barcoding of Stingless Bees (Hymenoptera: Meliponini) in Northern Peruvian Forests: A Plea for Integrative Taxonomy
Authors: Marconi Marilena , Modesti Alessandro , Alvarez Leydi Paz , Ogoña Paolo Villegas , Mendoza Agustín Cerna , Vecco-Giove Carlos Daniel , Luna Javier Ormeño , Di Giulio Andrea , Mancini Emiliano
Published: August 2022

Stingless bees (Hymenoptera: Meliponini) are among the most important pollinators of tropical forests. Peru is considered a hotspot of biodiversity of Meliponini, but many areas of this country (e.g., Peruvian Amazon) remain unexplored. We aimed to produce a first inventory of stingless bee species dwelling in humid and seasonally dry forests of northern Peru by combining traditional (morphologically-based) taxonomy and DNA barcoding. Specimens were collected in 2020 at five sites located in San Martin and Piura regions. We identified 12 genera of Meliponini. Among those, Trigona and Plebeia were the most abundant (45.9% and 12.8% respectively), whereas Nannotrigona and Scaura were the least represented ones (2.3%). We assigned a reliable species identification to about 30% of specimens (Trigona amazonensis, T. muzoensis, T. williana, Partamona testacea, Scaura tenuis, Tetragona goettei, and Tetragonisca angustula). Yet, more than a half of the specimens received a provisional identification (e.g., Geotrigona cf. fulvohirta, T. cf. amalthea, T. cf. fuscipennis, T. cf. hypogea, Melipona cf. cramptoni, Partamona cf. epiphytophila, Ptilotrigona cf. perenae, Scaura cf. latitarsis, Tetragona cf. clavipes, Trigonisca cf. atomaria). We also highlighted an extensive polyphyly that affected a number of currently recognized species (e.g., T. fulviventris, T. guianae, Plebeia franki, P. frontalis, M. eburnea, M. illota), whose members were split into various clades. Finally, 16% of individuals failed to be identified at the species level (Trigona sp. 1, T. sp. 2, Nannotrigona sp., Partamona sp., Scaptotrigona sp. 1, S. sp. 2, Trigonisca sp. 1, and Trigonisca sp. 2). We discuss our findings according to the current faunistic and biogeographic knowledge of Meliponini in Peru and the Neotropical region. We also remark on the importance of conducting a taxonomic revision of stingless bees and improving both their morphology-based identification keys and BOLD repository. Finally, we claim that integrative taxonomy shall be strongly implemented to truly assess the biodiversity of Neotropical stingless bees, allowing conserving these important pollinators and the associated traditional meliponiculture in an effective manner.
An HFman Probe-Based Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Simultaneous Detection of Hantaan and Seoul Viruses
Authors: Zeng Yi , Feng Yun , Zhao Yongjuan , Zhang Xiaoling , Yang Lifen , Wang Juan , Gao Zihou , Zhang Chiyu
Published: August 2022

Hantaviruses are zoonotic pathogens that are widely distributed worldwide. Hantaan virus (HTNV) and Seoul virus (SEOV) are two most common hantaviruses that infect humans and cause hemorrhagic fever with renal syndrome (HFRS). Rapid and sensitive detection of HTNV and SEOV are crucial for surveillance, clinical treatment and management of HFRS. This study aimed to develop a rapid HFman probe-based mulstiplex reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to simultaneously detect HTNV and SEOV. A novel multiplex RT-LAMP assay was developed, and 46 serum samples obtained from clinically suspected patients were used for evaluation. The novel RT-LAMP assay can detect as low as 3 copies/reaction of hantaviruses with a detection limit of 41 and 73 copies per reaction for HTNV and SEOV, respectively. A clinical evaluation showed that the consistencies of the multiplex RT-LAMP with RT-qPCR assay were 100% and 97.8% for HTNV and SEOV, respectively. In view of the high prevalence of HTNV and SEOV in rural areas with high rodent density, a colorimetric visual determination method was also developed for point-of-care testing (POCT) for the diagnosis of the two viruses. The novel multiplex RT-LAMP assay is a sensitive, specific, and efficient method for simultaneously detecting HTNV and SEOV.
Can the Concentration of Citric Acid Affect Its Cytotoxicity and Antimicrobial Activity?
Authors: Giardino Luciano , Generali Luigi , Savadori Paolo , Barros Mirela Cesar , de Melo Simas Leticia Lobo , Pytko-Polończyk Jolanta , Wilkoński Wojciech , Ballal Vasudev , Andrade Flaviana Bombarda de
Published: August 2022

Background: There has been no unanimity concerning the ideal concentration of citric acid for safe use in clinical practice. This study evaluated the cytotoxicity and the antibacterial activity in infected dentinal tubules of 10% and 1% citric acid (CA) solutions. Methods: The cytotoxicity of CA solutions in DMEM (diluted 1/10, 1/100) was assessed in L-929 fibroblasts. A broth macrodilution method (MIC and MBC) was used to assess CA antibacterial concentration. The antimicrobial activity of CA solutions was also evaluated after their final rinse inside root canals in previously Enterococcus faecalis-contaminated dentinal tubules. Ten infected dentine samples were rinsed for 5 min with 5% NaOCl and subsequently with 1% citric acid for 3 min. Another 10 were rinsed with 5% NaOCl and 10% citric acid for 3 min; the remaining four specimens were utilized as positive controls. Two uncontaminated specimens were used as negative controls. After LIVE/DEAD BacLight staining, the samples were assessed using CLSM to analyze the percentage of residual live and dead cells. Results: Both undiluted and diluted CA solutions showed severe toxicity; no changes from normal morphology were displayed when diluted 1/100. The MIC and MBC of CA were 6.25 mg/mL and 12.50 mg/mL, respectively. CA solutions demonstrated significantly low levels of bacterial counts than the positive control group, reporting a value of 9.3% for the 10% solution versus the 1% solution (35.2%). Conclusions: Despite its valuable antimicrobial properties, the cytotoxic effects of citric acid should be considered during endodontic treatment.
Radiographic Changes to Silver Diamine Fluoride Treated Carious Lesions after a Rinsing Step
Authors: Jabbour Zaher , Esmaeili Maryam , Hayashi Marc , Kim Reuben
Published: August 2022

Silver diamine fluoride (SDF) is radiopaque. This in vitro study compares the changes in the radiopacity of carious lesions after SDF application, potassium iodide (PI) application, and water rinse. Ten recently extracted human teeth were sectioned and divided into two groups (n = 10 in each group): Group 1 = SDF, Group 2 = SDF + PI. Teeth in Group 1 received SDF for 1 min and rinsed with 15 mL water. Group 2 received the same protocol with the addition of PI application for 1 min after SDF application. All samples were scanned with micro-computed tomography before SDF application, after SDF application, after PI application (group 2) and after water rinse. The radiopacity of the carious lesions increased significantly after SDF application in Group 1 and 2 (p < 0.017, p < 0.008, respectively). A significant increase in radiopacity after PI application was also observed in Group 2 (p < 0.008). Water rinsing significantly decreased the radiopacity in Group 1 and 2 (p < 0.017, p < 0.008, respectively), but the radiopacity remained significantly higher than the preoperative values (Group 1 p < 0.017, Group 2 p < 0.008). The radiopacity of carious lesions increases after SDF and SDF + PI applications. Water rinsing could reduce the radiopacity of SDF and SDF + PI treated carious lesions, and might reduce the content of SDF in carious lesions.
An Empirical Study of Students’ Perception of and Key Factors Affecting Overall Satisfaction in an Intensive Block Mode and Flipped Classroom
Authors: Kwan Paul , Memon Tayab D. , Hashmi Saad S. , Rhode Flemming , Kadel Rajan
Published: August 2022

Results of recent studies have suggested that intensive methods of delivery might improve engagement, attendance, and achievement for students from diverse backgrounds. Contributing to this area of inquiry, this study assesses how students perceived their experience studying a certificate course that was delivered in an online intensive block mode and flipped classroom (BMFC), pedagogy amidst COVID-19 restrictions. The subjects were students enrolled at Melbourne Institute of Technology between July 2021 and January 2022 across four certificate courses, three at postgraduate and one at undergraduate level. These certificate courses differed from normal degree courses in several aspects: (a) a shorter 4-week (undergraduate) or 5-week (postgraduate), instead of a 12-week duration, (b) subjects were taken sequentially instead of concurrently as in a normal semester, (c) taught using an online flipped classroom rather than the in-class approach, and (d) open to both high-school leavers and mature aged students who did not study full-time. A questionnaire involving 10 perception-based questions was used to survey students’ satisfaction with the BMFC delivery, in relation to their learning and engagement experience. The mean, median, and mode calculated from the responses revealed that students regarded the BMFC approach as more satisfied than not on a 5-star rating scale in 7 out of the 10 questions. This is further supported by high correlations among the questions (the lowest at r = 0.48 and the highest at r = 0.87). Multiple regression analysis using the first nine questions as predictors of the 10th question (overall satisfaction) revealed that six of these are statistically significant predictors (p < 0.05) of the overall satisfaction, implying that an increase in the overall satisfaction can potentially be achieved by improving these key factors of the BMFC delivered certificate courses. Our findings correlate with existing research that student learning and engagement might be improved by intensive modes of delivery. Furthermore, the BMFC pedagogy proposed in our study differentiates us from existing research, where block scheduling was used only in a face-to-face delivery in pre COVID-19 environment. Our study, therefore, contributes a novel delivery method for learning and teaching that is suitable for both online and face-to-face mode in a post COVID-19 era.
Metaverse, Crypto, and NFTs in Dentistry
Authors: Afrashtehfar Kelvin I. , Abu-Fanas Aiman S. H.
Published: August 2022

The dental community has expressed a growing interest in the metaverse and its applications [...]
Assessment of Curriculum Design by Learning Outcomes (LO)
Authors: Mendoza Walter , Ramírez Gabriel M. , González Claudio , Moreira Fernando
Published: August 2022

This paper analyzes the assessment experience as part of curriculum design by learning outcomes of the Master in User Experience Design of the Universidad Nacional Abierta y a Distancia—UNAD Colombia and the University of Lleida—UdL Spain. The article presents the assessment route, which allows for continuous improvement and is tailored to the self-assessment process. Conceptual references on curriculum design, competencies, purposes, constant improvement, and assessment are outlined for presentation. The theoretical line is based on international and national legal references. Likewise, the educational, pedagogical, and curricular implications of learning outcomes are presented, among them: change of paradigm (teaching vs. learning), coherence of curricular design, change of evaluation (qualification vs. assessment), decision-making, professor training, change of professor attitude, sustainability through assessment, and implementation routes, all of them with the aim of continuous improvement and to maintain the high quality of the program. One of the main conclusions indicates that curriculum design based on learning outcomes should be aligned and coherent at the macro-, meso-, and micro-curricular levels in order to meet the needs and requirements of the professional field.
Coplanar Asymmetry Transformer Distributed Modeling for X-Band Drive Power Amplifier Design on GaN Process
Authors: Fan Yihui , Wan Jing , Yang Zhe , Zhang Shengli , Zhao Jinxiang , Gao Gong , Zhang Xiaojie , Shen Haoyu , Xiao Nan , Zhang Yuying , Yan Yuepeng , Liang Xiaoxin
Published: August 2022

In this paper, a methodology for designing a distributed model for coplanar asymmetry transformer on gallium nitride (GaN) process is proposed, which can accurately characterize the transformer’s feature up to a millimeter-wave band. The paper analyses a transformer-based matching circuit and proposes a practical transformer design procedure. A two stage, transformer matching based X-band power amplifier (PA) is reported here. Using the proposed transformer model and correlated transformer design procedure can sharply reduce schematic design period and optimum process time. The PA chip is designed on a 0.25 µm GaN technology process and occupies a 1.515 mm2 area. At a 28 V supply, the gain and output power of the PA reaches 15 dB and 29 dBm respectively, and the wideband matching transformer reaches 47.6% bandwidth. To the best of our knowledge, the distributed model for coplanar asymmetry transformer and transformer-based X-band MMIC PA on GaN process in this work is the first case among the reported papers.
An Improved Multi-Objective Deep Reinforcement Learning Algorithm Based on Envelope Update
Authors: Hu Can , Zhu Zhengwei , Wang Lijia , Zhu Chenyang , Yang Yanfei
Published: August 2022

Multi-objective reinforcement learning (MORL) aims to uniformly approximate the Pareto frontier in multi-objective decision-making problems, which suffers from insufficient exploration and unstable convergence. We propose a multi-objective deep reinforcement learning algorithm (envelope with dueling structure, Noisynet, and soft update (EDNs)) to improve the ability of the agent to learn optimal multi-objective strategies. Firstly, the EDNs algorithm uses neural networks to approximate the value function and update the parameters based on the convex envelope of the solution boundary. Then, the DQN structure is replaced with the dueling structure, and the state value function is split into the dominance function and value function to make it converge faster. Secondly, the Noisynet method is used to add exploration noise to the neural network parameters to make the agent have a more efficient exploration ability. Finally, the soft update method updates the target network parameters to stabilize the training procedure. We use the DST environment as a case study, and the experimental results show that the EDNs algorithm has better stability and exploration capability than the EMODRL algorithm. In 1000 episodes, the EDNs algorithm improved the coverage by 5.39% and reduced the adaptation error by 36.87%.
Software Quality: How Much Does It Matter?
Authors: Kokol Peter
Published: August 2022

Interconnected computers and software systems have become an indispensable part of people’s lives in the period of digital transformation. Consequently, software quality research is becoming more and more critical. There have been multiple attempts to synthesise knowledge gained in software quality research; however, they were focused mainly on single aspects of software quality and did not structure the knowledge holistically. To fill this gap, we harvested software quality publications indexed in the Scopus bibliographic database. We analysed them using synthetic content analysis which is a triangulation of bibliometrics and content analysis. The search resulted in 15,468 publications. The performance bibliometric analysis showed that the production of research publications relating to software quality is currently following an exponential growth trend and that the software quality research community is growing. The most productive country was the United States, followed by China. The synthetic content analysis revealed that the published knowledge could be structured into six themes, the most important being the themes regarding software quality improvement by enhancing software engineering, advanced software testing and improved defect and fault prediction with machine learning and data mining.
A Hybrid Multimodal Data Fusion-Based Method for Identifying Gambling Websites
Authors: Wang Chenyang , Zhang Min , Shi Fan , Xue Pengfei , Li Yang
Published: August 2022

With the development of network technology, the number of gambling websites has grown dramatically, causing a threat to social stability. There are many machine learning-based methods are proposed to identify gambling websites by analyzing the URL, the text, and the images of the websites. Nevertheless, most of the existing methods ignore one important piece of information, i.e., the text within the website images. Only the visual features of images are extracted for detection, while the semantic features of texts on the images are ignored. However, these texts have key information clearly pointing to gambling websites, which can help us identify such websites more accurately. Therefore, how to fuse image and text multimodal data is a challenge that should be met.Motivated by this, in this paper, we propose a hybrid multimodal data fusion-based method for identifying gambling websites by extracting and fusing visual and semantic features of the website screenshots. First, we fine tune the pretrained ResNet34 model to train an image classifier and to extract visual features of webpage screenshots. Second, we extract textual content from webpage screenshots through the optical character recognition (OCR) technique. We use pretrained Word2Vec word vectors as the initial embedding layer and use Bi-LSTM to train a text classifier and extract semantic features of textual content on the screenshots. Third, we use self-attention to fuse the visual and semantic features and train a multimodal classifier. The prediction results of image, text, and multimodal classifiers are fused by the late fusion method to obtain the final prediction result. To demonstrate the effectiveness of the proposed method, we conduct experiments on the webpage screenshot dataset we collected. The experimental results indicate that OCR text on the webpage screenshots has strong semantic features and the proposed hybrid multimodal data fusion based method can effectively improve the performance in identifying gambling websites, with accuracy, precision, recall, and F1-score all over 99%.
A Review on Human–Robot Proxemics
Authors: Samarakoon S. M. Bhagya P. , Muthugala M. A. Viraj J. , Jayasekara A. G. Buddhika P.
Published: August 2022

An emerging trend in utilizing service robots in a vast range of application areas could be seen nowadays as a promising effort to uplift the living standard. These service robots are intended to be used by non-expert users, and their service tasks often require navigation in human-populated environments. Thus, human-friendly navigation behavior is expected from these robots by users. A service robot should be aware of Human–Robot Proxemics (HRP) to facilitate human-friendly navigation behavior. This paper presents a review on HRP. Both user studies conducted for exploring HRP preferences and methods developed toward establishing HRP awareness in service robots are considered within the scope of the review. The available literature has been scrutinized to identify the limitations of state of the art and potential future work. Furthermore, important HRP parameters and behavior revealed by the existing user studies are summarized under one roof to smooth the availability of data required for developing HRP-aware behavior in service robots.
Mitigating Misfire and Fire-through Faults in Hybrid Renewable Energy Systems Utilizing Dynamic Voltage Restorer
Authors: Osama abed elraouf M. , Aljohani Mansour , Mosaad Mohamed I. , AbdulFattah Tarek A.
Published: August 2022

Recently, there was a great focus on integrating renewable energy sources (RESs) into electrical power systems (hybrid systems) due to their many environmental and economic advantages. The output of most of these RESs is DC; some power electronic devices, including inverters, must be used to integrate these RESs into the electrical grid. Any maloperation, faults, or improper control in these power electronic devices will enormously affect these hybrid systems’ performance. This paper aims to mitigate the misfire and fire-through faults that occur at the switching of the inverter that connects three renewable sources: PV, wind, and the fuel cell to the grid. This mitigation of such inverter faults (misfire and fire-through) is performed through optimal tuning of the PI controller driving a dynamic voltage restorer (DVR) connected at the system’s AC side. The optimization technique used is particle swarm optimization (PSO). While mitigating these two inverter faults using the PI-PSO controller for the DVR, improved system performance through voltages, currents, and powers waveforms is achieved. Besides, the three renewable sources were kept in continuous operation without disconnection from the system during these faults.
Raw Jojoba Oil as a Sustainable Fuel to Diesel Engines and Comparison with Diesel Fuel
Authors: Selim Mohamed Y. E. , Ghannam Mamdouh T. , Abdo Bishoy N. , Attai Youssef A. , Radwan Mohsen S.
Published: August 2022

Raw Jojoba oil was used in a direct-injection diesel engine without any engine modifications and compared with both diesel fuel and 50/50 raw Jojoba/diesel. The measured parameters included the rheological properties measured in the range of shear rate from 100 to 500 1/s. Distillation data were presented for raw Jojoba oil compared to diesel. The parameters included exhaust gas analysis, block vibration, sound noise, and the combustion pressure and its rise rate. Averaged pressure-crank angle, vibration, and its frequency spectrum and sound level were presented. Data also included brake power, specific fuel consumption, and exhaust temperatures for all fuels used. For Jojoba oil, the engine did not exhibit low power output or specific fuel consumption. Exhaust temperatures, smoke opacity, and NOx emissions were lower than diesel case. Noise for Jojoba was higher than diesel case. The engine block vibration was concentrated towards the low frequency range.
Infrastructure Safety from the Perspective of Resilience Theory
Authors: Cao Xu-Yang , Xu Ji-Gang , Feng De-Cheng
Published: August 2022

With the rapid development of urbanization, the scales of urban population and land use are tremendously expanding [...]
Co-Firing of Refuse-Derived Fuel with Ekibastuz Coal in a Bubbling Fluidized Bed Reactor: Analysis of Emissions and Ash Characteristics
Authors: Suleimenova Botakoz , Aimbetov Berik , Zhakupov Daulet , Shah Dhawal , Sarbassov Yerbol
Published: August 2022

Converting municipal solid waste (MSW) into valuable feedstocks, such as refuse-derived fuel (RDF), is a sustainable method according to the concept of waste management hierarchy. A heterogeneous composition with a good calorific value and lower emissions allows RDF to be used for energy recovery purposes. We have earlier analyzed the generation and thermochemical characteristics of the MSW produced in Kazakhstan. This work aims to study the combustion characteristics in terms of emissions and ash composition to evaluate the possibility of RDF co-firing with Ekibastuz coal. In particular, RDF is blended with high ash bituminous coal (Ekibastuz coal) and co-fired in the laboratory scale bubbling fluidized bed reactor (BFB) at a bed temperature of 850 °C. The co-firing tests of RDF to coal samples were conducted under various proportions to analyze flue gas compositions. Experiments were carried in the presence of bed material (sand), and the fuel particles were fed in batch mode into the hot riser. The BFB reactor had a height of 760 mm and internal diameter of 48 mm. The gaseous products in the flue gas were analyzed by FTIR spectrometry (Gasmet Dx4000). Ash composition was examined by XRD, XRF, SEM, and PSD. The results showed that a high RDF content decreased SO2 emissions to 28 ppm, while it negatively affected NOx release to 1400 ppm, owing to excess air. The emissions of gases from different blended samples and mineral transformations were investigated and discussed in this study.
Intra- and Interspecific Variability of Non-Structural Carbohydrates and Phenolic Compounds in Flowers of 70 Temperate Trees and Shrubs
Authors: Paź-Dyderska Sonia , Żytkowiak Roma , Jagodziński Andrzej M.
Published: August 2022

(1) The focus on floral functional traits and their variability has been significantly lower than when compared to other plant organs. Here, we focused on the variability of four novel floral chemical traits. We aimed to assess the level of interspecific variability of total non-structural carbohydrates (TNC) and total phenolic compounds (TPh) in the flowers of woody species. (2) We collected data on 70 species of temperate trees and shrubs. We also assessed the intraspecific level of variability by collecting flowers from the high-light and low-light parts of the crown. (3) We found a phylogenetic signal in the variability of starch and TPh. We did not observe statistically significant differences or biologically significant trends between the high-light and low-light parts of the crown. We detected a low impact of light availability on the intraspecimen variability of the TNC and TPh contents. (4) Low intraspecimen variability allows for a more reliable extrapolation of measurements in cases of interspecific comparisons and can be used to better describe the reproductive strategies of different woody species.
Transcriptome Analysis of Developing Xylem Provides New Insights into Shade Response in Three Poplar Hybrids
Authors: Zhang Min , Zhou Xinglu , Xiang Xiaodong , Du Changjian , Ge Xiaolan , Du Jiujun , Zhang Lei , Hu Jianjun
Published: August 2022

Poplars have a strong response to light, and shade is one of the main environmental factors that limits the growth and development of poplars. Exploring the response mechanism of the developing xylem of poplar under shady conditions is of great reference significance for improving wood yields. In this study, three excellent hybrids of poplar (Populus euramericana ‘Zhonglin46’ (Pe), Populus deltoides ‘27-17’ (Pd), Populus × ‘Wq156’ (Pw) were studied under shady conditions. Based on the phenotypic data and developing a xylem transcriptome analysis, the molecular mechanism of poplars’ response to shade was preliminarily revealed, and the core regulatory genes responding to shade were identified by a weighted co-expression network analysis (WGCNA). The results showed that Pw growth was significantly affected by shade, while Pe growth was slightly affected by shade. An enrichment analysis of 13,675 differentially expressed genes (DEGs) found that shade affected the expression of genes related to the glutathione metabolic pathway. The WGCNA analysis identified two modules (“Brown” and “Purple”) related to the shade response and discovered seven hub genes. These hub genes were related to xylem development, vascular cambium division, stomatal development, and phytochrome A signal transduction. These results provide important basic information for gaining insight into the molecular response to shade in different poplar hybrids.
Interactive Effects of Copper and Functional Substances in Wine on Alcoholic Hepatic Injury in Mice
Authors: Sun Xiangyu , Wang Jiaqi , Ge Qian , Li Caihong , Ma Tingting , Fang Yulin , Zhan Jicheng
Published: August 2022

This study analyzed the interaction between copper and functional substances in wine under different drinking amounts on alcoholic liver injury in mice. When the daily drinking amount reached 500 mL/60 kg/day (14% abv) with just ethyl alcohol, the liver aspartate aminotransferase, alanine aminotransferase, and total triglyceride levels of mice were significantly increased to 130.71 U/L, 37.45 U/L, 2.55 U/L, the total antioxidant capacity, catalase, and glutathione level decreased significantly to 1.01 U/mL, 30.20 U/mgprot, and 2.10 U/mgprot, and the liver became gradually damaged. Wine could alleviate and reduce the damage caused by ethyl alcohol well. Low concentrations of copper (0.33, 0.66 mg/L) in wine hardly caused hepatic injury in mice and only significantly improved the aspartate aminotransferase values (109.21 U/L, 127.29 U/L) of serum. Combined with the staining evidence, in the case of medium and high intragastric doses (≥500 mL/60 kg/day), 0.99 mg/L copper (the maximum allowed by China’s national standards) in wine began to damage the liver, indicating that under this concentration, the damage of copper to the liver had begun to exceed the protective effect of wine’s functional substances on alcoholic hepatic injury. At all experimental doses, high concentrations (1.33 mg/L, 2.00 mg/L) of copper significantly aggravated alcoholic hepatic injury in mice, indicating that high concentrations of copper have a great toxicological risk. In the future, it is necessary to further strengthen the control of copper content in wine and the inspection of market wines in order to protect the health of consumers.
Ion Chromatography–High-Resolution Mass Spectrometry Method for the Determination of Bromide Ions in Cereals and Legumes: New Scenario for Global Food Security
Authors: Rocchi Rachele , Rosato Roberta , Bellocci Mirella , Migliorati Giacomo , Scarpone Rossana
Published: August 2022

The new scenario for global food production and supply is decidedly complex given the current forecast of an increase in food fragility due to international tensions. In this period, exports from other parts of the world require different routes and treatments to preserve the food quality and integrity. Fumigation is a procedure used for the killing, removal, or rendering infertile of pests, with serious dangers to human health. The most-used fumigants are methyl bromide and ethylene dibromide. It is important to bear in mind that the soil may contain bromide ions naturally or from anthropogenic source (fertilizers and pesticides that contain bromide or previous fumigations). Different methods (titrimetric, spectrophotometric, and fluorometric approaches) are available to rapidly determine the amount of bromide ion on site in the containers, but these are non-specific and with high limits of quantification. The increasing interest in healthy food, without xenobiotic residues, requires the use of more sensitive, specific, and accurate analytical methods. In order to help give an overview of the bromide ion scenario, a new, fast method was developed and validated according to SANTE 11312/2021. It involves the determination of bromide ion in cereals and legumes through ion chromatography–Q-Orbitrap. The extraction was performed by the QuPPe method, but some modifications were applied based on the matrix. The method described here was validated at four different levels. Recoveries were satisfactory and the mean values ranged between 99 and 106%, with a relative standard deviation lower than 3%. The linearity in the matrix was evaluated to be between 0.010 and 2.5 mg kg−1, with a coefficient of determination (R2) of 0.9962. Finally, the proposed method was applied to different cereals and legumes (rice, wheat, beans, lentils pearled barley, and spelt) and tested with satisfactory results in EUPT-SMR16 organized by EURL.
Consumers’ Awareness, Behavior and Expectations for Food Packaging Environmental Sustainability: Influence of Socio-Demographic Characteristics
Authors: Chirilli Chiara , Molino Martina , Torri Luisa
Published: August 2022

Packaging is a leading factor determining the total environmental effect of food products. This study investigated consumers’ awareness, behavior and expectations in relation to the environmental sustainability aspects of food packaging. Using an online survey, responses from 646 participants were collected. The effect of socio-demographic characteristics on all variable responses was explored by ANOVA models and t-tests. Participants were segmented according to a visual approach based on a principal component analysis applied on the consumers’ behavioral data. Gender, age, and education level affected consumer awareness, behavior and expectations differently. Four groups of consumers were distinguished on the grounds of their behavior in relation to food packaging: (1) More sustainable—packaging-role-oriented; (2) More sustainable—packaging minimizers; (3) Less sustainable; and (4) Medium sustainable. The most sustainable groups were mainly composed of females, while less sustainable consumers were mainly the youngest. The four groups differed in terms of expectations for sustainability-related information that can be communicated through food labels. In conclusion, this work provided new knowledge that is useful to understand the factors that influence consumer behavior and to promote the consumers’ packaging-related sustainability choices through food packaging.