bluuebunny/crossref_metadata_2025 huggingface dataset:
Unravelling the Gut Microbiome Role in Cardiovascular Disease: A Systematic Review and a Meta-Analysis
Authors: Martins Diana , Silva Cláudia , Ferreira António Carlos , Dourado Sara , Albuquerque Ana , Saraiva Francisca , Batista Ana Beatriz , Castro Pedro , Leite-Moreira Adelino , Barros António S. , Miranda Isabel M.
Published: June 2024

A notable shift in understanding the human microbiome’s influence on cardiovascular disease (CVD) is underway, although the causal association remains elusive. A systematic review and meta-analysis were conducted to synthesise current knowledge on microbial taxonomy and metabolite variations between healthy controls (HCs) and those with CVD. An extensive search encompassing three databases identified 67 relevant studies (2012–2023) covering CVD pathologies from 4707 reports. Metagenomic and metabolomic data, both qualitative and quantitative, were obtained. Analysis revealed substantial variability in microbial alpha and beta diversities. Moreover, specific changes in bacterial populations were shown, including increased Streptococcus and Proteobacteria and decreased Faecalibacterium in patients with CVD compared with HC. Additionally, elevated trimethylamine N-oxide levels were reported in CVD cases. Biochemical parameter analysis indicated increased fasting glucose and triglycerides and decreased total cholesterol and low- and high-density lipoprotein cholesterol levels in diseased individuals. This study revealed a significant relationship between certain bacterial species and CVD. Additionally, it has become clear that there are substantial inconsistencies in the methodologies employed and the reporting standards adhered to in various studies. Undoubtedly, standardising research methodologies and developing extensive guidelines for microbiome studies are crucial for advancing the field.
The Rhododendron Chrysanthum Pall.s’ Acetylation Modification of Rubisco Enzymes Controls Carbon Cycling to Withstand UV−B Stress
Authors: Liu Meiqi , Gong Fushuai , Yu Wang , Cao Kun , Xu Hongwei , Zhou Xiaofu
Published: June 2024

Lysine acetylation of proteins plays a critical regulatory function in plants. A few advances have been made in the study of plant acetylproteome. However, until now, there have been few data on Rhododendron chrysanthum Pall. (R. chrysanthum). We analyzed the molecular mechanisms of photosynthesis and stress resistance in R. chrysanthum under UV−B stress. We measured chlorophyll fluorescence parameters of R. chrysanthum under UV−B stress and performed a multi−omics analysis. Based on the determination of chlorophyll fluorescence parameters, R. chrysanthum Y(NO) (Quantum yield of non−photochemical quenching) increased under UV−B stress, indicating that the plant was damaged and photosynthesis decreased. In the analysis of acetylated proteomics data, acetylated proteins were found to be involved in a variety of biological processes. Notably, acetylated proteins were significantly enriched in the pathways of photosynthesis and carbon fixation, suggesting that lysine acetylation modifications have an important role in these activities. Our findings suggest that R. chrysanthum has decreased photosynthesis and impaired photosystems under UV−B stress, but NPQ shows that plants are resistant to UV−B. Acetylation proteomics revealed that up- or down-regulation of acetylation modification levels alters protein expression. Acetylation modification of key enzymes of the Calvin cycle (Rubisco, GAPDH) regulates protein expression, making Rubisco and GAPDH proteins expressed as significantly different proteins, which in turn affects the carbon fixation capacity of R. chrysanthum. Thus, Rubisco and GAPDH are significantly differentially expressed after acetylation modification, which affects the carbon fixation capacity and thus makes the plant resistant to UV−B stress. Lysine acetylation modification affects biological processes by regulating the expression of key enzymes in photosynthesis and carbon fixation, making plants resistant to UV−B stress.
Roles of Mitochondrial Dysfunction in Diabetic Kidney Disease: New Perspectives from Mechanism to Therapy
Authors: Yang Yichen , Liu Jiahui , Shi Qiling , Guo Buyu , Jia Hanbing , Yang Yuxuan , Fu Songbo
Published: June 2024

Diabetic kidney disease (DKD) is a common microvascular complication of diabetes and the main cause of end-stage renal disease around the world. Mitochondria are the main organelles responsible for producing energy in cells and are closely involved in maintaining normal organ function. Studies have found that a high-sugar environment can damage glomeruli and tubules and trigger mitochondrial dysfunction. Meanwhile, animal experiments have shown that DKD symptoms are alleviated when mitochondrial damage is targeted, suggesting that mitochondrial dysfunction is inextricably linked to the development of DKD. This article describes the mechanisms of mitochondrial dysfunction and the progression and onset of DKD. The relationship between DKD and mitochondrial dysfunction is discussed. At the same time, the progress of DKD treatment targeting mitochondrial dysfunction is summarized. We hope to provide new insights into the progress and treatment of DKD.
Concept of Normativity in Multi-Omics Analysis of Axon Regeneration
Authors: Moceri Isabella , Meehan Sean , Gonzalez Emily , Park Kevin K. , Hackam Abigail , Lee Richard K. , Bhattacharya Sanjoy
Published: June 2024

Transcriptomes and proteomes can be normalized with a handful of RNAs or proteins (or their peptides), such as GAPDH, β-actin, RPBMS, and/or GAP43. Even with hundreds of standards, normalization cannot be achieved across different molecular mass ranges for small molecules, such as lipids and metabolites, due to the non-linearity of mass by charge ratio for even the smallest part of the spectrum. We define the amount (or range of amounts) of metabolites and/or lipids per a defined amount of a protein, consistently identified in all samples of a multiple-model organism comparison, as the normative level of that metabolite or lipid. The defined protein amount (or range) is a normalized value for one cohort of complete samples for which intrasample relative protein quantification is available. For example, the amount of citrate (a metabolite) per µg of aconitate hydratase (normalized protein amount) identified in the proteome is the normative level of citrate with aconitase. We define normativity as the amount of metabolites (or amount range) detected when compared to normalized protein levels. We use axon regeneration as an example to illustrate the need for advanced approaches to the normalization of proteins. Comparison across different pharmacologically induced axon regeneration mouse models entails the comparison of axon regeneration, studied at different time points in several models designed using different agents. For the normalization of the proteins across different pharmacologically induced models, we perform peptide doping (fixed amounts of known peptides) in each sample to normalize the proteome across the samples. We develop Regen V peptides, divided into Regen III (SEB, LLO, CFP) and II (HH4B, A1315), for pre- and post-extraction comparisons, performed with the addition of defined, digested peptides (bovine serum albumin tryptic digest) for protein abundance normalization beyond commercial labeled relative quantification (for example, 18-plex tandem mass tags). We also illustrate the concept of normativity by using this normalization technique on regenerative metabolome/lipidome profiles. As normalized protein amounts are different in different biological states (control versus axon regeneration), normative metabolite or lipid amounts are expected to be different for specific biological states. These concepts and standardization approaches are important for the integration of different datasets across different models of axon regeneration.
An Early Gestation Plasma Inflammasome in Rural Bangladeshi Women
Authors: Kim Hyunju , Bedsaul-Fryer Jacquelyn R. , Schulze Kerry J. , Sincerbeaux Gwen , Baker Sarah , Rebholz Casey M. , Wu Lee SF , Gogain Joseph , Cuddeback Lena , Yager James D. , De Luca Luigi M. , Siddiqua Towfida J. , West Keith P.
Published: June 2024

Circulating α1-acid glycoprotein (AGP) and C-reactive protein (CRP) are commonly measured to assess inflammation, but these biomarkers fail to reveal the complex molecular biology of inflammation. We mined the maternal plasma proteome to detect proteins that covary with AGP and CRP. In 435 gravida predominantly in <12-week gestation, we correlated the relative quantification of plasma proteins assessed via a multiplexed aptamer assay (SOMAScan®) with AGP and CRP, quantified by immunoassay. We defined a plasma inflammasome as protein correlates meeting a false discovery rate <0.05. We examined potential pathways using principal component analysis. A total of 147 and 879 of 6431 detected plasma proteins correlated with AGP and CRP, respectively, of which 61 overlapped with both biomarkers. Positive correlates included serum amyloid, complement, interferon-induced, and immunoregulatory proteins. Negative correlates were micronutrient and lipid transporters and pregnancy-related anabolic proteins. The principal components (PCs) of AGP were dominated by negatively correlated anabolic proteins associated with gestational homeostasis, angiogenesis, and neurogenesis. The PCs of CRP were more diverse in function, reflecting cell surface and adhesion, embryogenic, and intracellular and extra-hepatic tissue leakage proteins. The plasma proteome of AGP or CRP reveals wide proteomic variation associated with early gestational inflammation, suggesting mechanisms and pathways that merit future research.
Exploring the Impact of Short Term Travel on Gut Microbiota and Probiotic Bacteria Mediated Stability
Authors: Zhao Yiming , Li Chunyan , Wu Kaijuan , Chen Hao , Wang Qingqun , Xiao Ying , Yao Siqi , Hong Ao , Zhang Man , Lei Shibo , Yang Wenyu , Zhong Shukun , Umar Abdulrahim , Huang Jing , Yu Zheng
Published: June 2024

Although travelers are frequently accompanied by abdominal discomfort and even diarrhea, not every trip can cause this issue. Many studies have reported that intestinal microbes play an important role in it. However, little is known about the reason for the dynamics of these intestinal microbes. Here, we delved into the effects of short-term travel on the gut microbiota of 12 healthy individuals. A total of 72 fecal samples collected before and after one-week travel, alongside non-traveling controls, underwent amplicon sequencing and a series of bioinformatic analyses. We found that travel significantly increased intra-individual gut microbiota fluctuations without diarrhea symptoms. In addition, the initial composition of the gut microbiota before travel emerged as a crucial factor in understanding these fluctuations. Travelers with stable microbiota exhibited an enrichment of specific probiotic bacteria (Agathobaculum, Faecalibacterium, Bifidobacterium, Roseburia, Lactobacillus) before travel. Another batch of data validated their predictive role in distinguishing travelers with and without the gut microbial disorder. This work provided valuable insights into understanding the relationship between gut microbiota and travel. It offered a microbiota-centric perspective and a potential avenue for interventions to preserve gut health during travel.
Analgesic Effect of SKI306X on Chronic Postischemic Pain and Spinal Nerve Ligation-Induced Neuropathic Pain in Mice
Authors: Quan Jie , He Chun Jing , Kim Ji Yeon , Lee Jin Young , Kim Chang Jae , Jeon Young Jae , Im Chang Woo , Lee Do Kyung , Kim Ji Eun , Park Hue Jung
Published: June 2024

Neuropathic pain (NP) results from lesions or diseases affecting the peripheral or central somatosensory system. However, there are currently no drugs that are particularly effective in treating this condition. SKI306X is a blend of purified extracts of three oriental herbs (Clematis mandshurica, Trichosanthes kirilowii, and Prunella vulgaris) commonly used to treat osteoarthritis for their chondroprotective effects. Chronic postischemic pain (CPIP) and spinal nerve ligation (SNL) models were created by binding the upper left ankle of mice with an O-ring for 3 h and ligating the L5 spinal nerve, respectively. Mice with allodynia were injected intraperitoneally with 0.9% normal saline (NS group) or different doses (25, 50, or 100 mg/kg) of SKI306X (SKI groups). We assessed allodynia using von Frey filaments before injection and 30, 60, 90, 120, 180, and 240 min and 24 h after injection to confirm the antiallodynic effect of SKI306X. We also measured glial fibrillary acidic protein (GFAP) levels in the spinal cord and dorsal root ganglia to confirm the change of SKI306X administration. Both models exhibited significant mechanical allodynia. The intraperitoneal injection of SKI306X significantly increased the paw withdrawal threshold in a dose-dependent manner, as the paw withdrawal threshold was significantly increased after SKI306X administration compared with at baseline or after NS administration. GFAP levels in the SKI group decreased significantly (p < 0.05). Intraperitoneal administration of SKI306X dose-dependently attenuated mechanical allodynia and decreased GFAP levels, suggesting that GFAP is involved in the antiallodynic effect of SKI306X in mice with CPIP and SNL-induced NP.
Oxidized Cholesterol Derivatives in Fraction B Prepared from Gulf Catfish (Arius bilineatus, Val.) Skin Regulate Calcium Response and Neutrophil Extracellular Traps (NETs) Formation
Authors: Al-Hassan Jassim M. , Afzal Mohammad , Oommen Sosamma , Liu Yuan Fang , Khan Meraj , Pace-Asciak Cecil
Published: June 2024

In this study, we present in vitro actions of pure commercial preparations of oxidized and/or dehydrated metabolites of cholesterol (OS) identified in the lipid fraction of Fraction B (FB) prepared from a catfish skin preparation on calcium transients and on the formation of human neutrophil extracellular traps (NETs). These investigations are part of an ongoing effort to understand the important roles these compounds play as components of FB when FB is applied to accelerate the healing of wounds and the healing of highly infected non-healing diabetic foot ulcers, without the use of antibiotics. Our aim was to determine potential therapeutic interventions for various disease states. Our results reveal interesting findings, demonstrating specific actions of the individual compounds. Compounds 7α-hydroxy-cholesterol (S3), Cholestane-3,5,6-triol (S5), 5-cholesten-3β-ol-7-one (S8) and Cholesta-3,5 dien-7-one (S10) are inhibitory, while Cholesterol 5β,6β-epoxide (S4) and 5α-cholestane-3,6-dione (S11) activate the response for calcium influx in human neutrophils. A somewhat similar response is observed in dHL60 cell lines, where S3, S5, S7, S8, and cholesta-2,4-diene (S14) inhibit the calcium influx, although S4, S10, and S11 activate the response in this cell line. Furthermore, we observed a relationship between actions against NETosis and calcium transients. Interestingly, relative to the vehicle control, S3, Cholesta-3,5 diene (S9), and S14 appeared to significantly stimulate DNA release (NETosis), while S2, 7α-hydroxy-cholesterol (S6) and cholesta-3,5 dien-7-one (S10) caused lesser stimulation. We provide the IC50 activities for each compound tested in each assay. Calcium influx and NETs formation (NETosis) correlate with diseases exacerbation. These findings offer valuable insights into the potential therapeutic applications of individual OS for various diseases, highlighting their importance in future interventions.
Hyperbaric Oxygen Therapy as a Novel Approach to Modulating Macrophage Polarization for the Treatment of Glioblastoma
Authors: Yuen Chun-Man , Tsai Hung-Pei , Tseng Tzu-Ting , Tseng Yu-Lung , Lieu Ann-Shung , Kwan Aij-Lie , Chang Alice Y. W.
Published: June 2024

Glioblastoma multiforme (GBM) is a highly aggressive brain cancer with a poor prognosis despite current treatments. This is partially attributed to the immunosuppressive environment facilitated by tumor-associated macrophages, which predominantly underlie the tumor-promoting M2 phenotype. This study investigated the potential of hyperbaric oxygen (HBO) therapy, traditionally used to treat conditions such as decompression sickness, in modulating the macrophage phenotype toward the tumoricidal M1 state and disrupting the supportive tumor microenvironment. HBO has direct antiproliferative effects on tumor cells and reduces hypoxia, which may impair angiogenesis and tumor growth. This offers a novel approach to GBM treatment by targeting the role of the immune system within the tumor microenvironment. The effects of HBO on macrophage polarization and GBM cell viability and apoptosis were evaluated in this study. We detected that HBO promoted M1 macrophage cytokine expression while decreasing GBM cell viability and increasing apoptosis using GBM cell lines and THP-1-derived macrophage-conditioned media. These findings suggest that HBO therapy can shift macrophage polarization toward a tumoricidal M1 state. This can improve GBM cell survival and offers a potential therapeutic strategy. In conclusion, HBO can shift macrophages from a tumor-promoting M2 phenotype to a tumoricidal M1 phenotype in GBM. This can facilitate apoptosis and, in turn, improve treatment outcomes.
Circulating Nucleosomes as a Novel Biomarker for Sepsis: A Scoping Review
Authors: Su Fuhong , Moreau Anthony , Savi Marzia , Salvagno Michele , Annoni Filippo , Zhao Lina , Xie Keliang , Vincent Jean-Louis , Taccone Fabio Silvio
Published: June 2024

Circulating nucleosome levels are commonly elevated in physiological and pathological conditions. Their potential as biomarkers for diagnosing and prognosticating sepsis remains uncertain due, in part, to technical limitations in existing detection methods. This scoping review explores the possible role of nucleosome concentrations in the diagnosis, prognosis, and therapeutic management of sepsis. A comprehensive literature search of the Cochrane and Medline libraries from 1996 to 1 February 2024 identified 110 potentially eligible studies, of which 19 met the inclusion criteria, encompassing a total of 39 SIRS patients, 893 sepsis patients, 280 septic shock patients, 117 other ICU control patients, and 345 healthy volunteers. The enzyme-linked immunosorbent assay [ELISA] was the primary method of nucleosome measurement. Studies consistently reported significant correlations between nucleosome levels and other NET biomarkers. Nucleosome levels were higher in patients with sepsis than in healthy volunteers and associated with disease severity, as indicated by SOFA and APACHE II scores. Non-survivors had higher nucleosome levels than survivors. Circulating nucleosome levels, therefore, show promise as early markers of NETosis in sepsis, with moderate diagnostic accuracy and strong correlations with disease severity and prognosis. However, the available evidence is drawn mainly from single-center, observational studies with small sample sizes and varied detection methods, warranting further investigation.
Design of an SMA-Based Actuator for Replicating Normal Gait Patterns in Pediatric Patients with Cerebral Palsy
Authors: Mansilla Navarro Paloma , Copaci Dorin , Arias Janeth , Blanco Rojas Dolores
Published: June 2024

Cerebral Palsy refers to a group of incurable motor disorders affecting 0.22% of the global population. Symptoms are managed by physiotherapists, often using rehabilitation robotics. Exoskeletons, offering advantages over conventional therapies, are evolving to be more wearable and biomimetic, requiring new flexible actuators that mimic human tissue. The main objective behind this article is the design of a flexible exosuit based on shape-memory-alloy-based artificial muscles for pediatric patients that replicate the walking cycle pattern in the ankle joint. Thus, four shape-memory-alloy-based actuators were sewn to an exosuit at the desired actuation points and controlled by a two-level controller. The loop is closed through six inertial sensors that estimate the real angular position of both ankles. Different frequencies of actuation have been tested, along with the response of the actuators to different walking cycle patterns. These tests have been performed over long periods of time, comparing the reference created by a reference generator based on pediatric walking patterns and the response measured by the inertial sensors. The results provide important measurements concerning errors, working frequencies and cooling times, proving that this technology could be used in this and similar applications and highlighting its limitations.
Birds and People: A Symbiotic Relationship in Practice
Authors: Butler Richard William
Published: June 2024

This Special Issue of Birds is focused on a number of ways in which people and birds interact with nature, and the example discussed here incorporates four of the seven relationships noted. These are: how birds and birding connect people with nature, the role of communities in the study and conservation of birds, the involvement of people with professional ornithologists, and citizens’ perception and knowledge of birds. The island of Fair Isle provides the location for the examination of these relationships, illustrating the positive interaction between the two human populations of the island and the two avian populations. It is based on fieldwork and literature studies conducted at intervals over a sixty-year period and a review of written and photographic evidence dating back to the beginning of the twentieth century. The purpose of the paper therefore, is to discuss how the relationships between the human and avian populations of the island have changed over time to a more positive and mutually dependent relationship, which is somewhat unique and can be described as symbiotic.
Automated Pupillometry Is Able to Discriminate Patients with Acute Stroke from Healthy Subjects: An Observational, Cross-Sectional Study
Authors: Scala Irene , Miccoli Massimo , Pafundi Pia Clara , Rizzo Pier Andrea , Vitali Francesca , Bellavia Simone , Giovanni Jacopo Di , Colò Francesca , Marca Giacomo Della , Guglielmi Valeria , Brunetti Valerio , Broccolini Aldobrando , Di Iorio Riccardo , Monforte Mauro , Calabresi Paolo , Frisullo Giovanni
Published: June 2024

Background: Automated pupillometry (AP) is a handheld, non-invasive tool that is able to assess pupillary light reflex dynamics and is useful for the detection of intracranial hypertension. Limited evidence is available on acute ischemic stroke (AIS) patients. The primary objective was to evaluate the ability of AP to discriminate AIS patients from healthy subjects (HS). Secondly, we aimed to compute a predictive score for AIS diagnosis based on clinical, demographic, and AP variables. Methods: We included 200 consecutive patients admitted to a comprehensive stroke center who underwent AP assessment through NPi-200 (NeurOptics®) within 72 h of stroke onset and 200 HS. The mean values of AP parameters and the absolute differences between the AP parameters of the two eyes were considered in the analyses. Predictors of stroke diagnosis were identified through univariate and multivariate logistic regressions; we then computed a nomogram based on each variable’s β coefficient. Finally, we developed a web app capable of displaying the probability of stroke diagnosis based on the predictive algorithm. Results: A high percentage of pupil constriction (CH, p < 0.001), a low constriction velocity (CV, p = 0.002), and high differences between these two parameters (p = 0.036 and p = 0.004, respectively) were independent predictors of AIS. The highest contribution in the predictive score was provided by CH, the Neurological Pupil Index, CV, and CV absolute difference, disclosing the important role of AP in the discrimination of stroke patients. Conclusions: The results of our study suggest that AP parameters, and in particular, those concerning pupillary constriction, may be useful for the early diagnosis of AIS.
Identifying the Qualities of Attention and the Attentional Style in Indoor Team Sports: A Gender Comparison
Authors: Badau Adela
Published: June 2024

Attention is an essential psychological component in sports games, which conditions sports success. The purpose of this study was to identify the attention style (internal or external) and the weight of attention qualities depending on the practiced team sport (basketball, volleyball, or handball) and gender (female or male), in athletes aged 15–18. A total of 177 active athletes (87 female (mean age ± standard deviation: 16.07 ± 0.94 years) and 90 male (mean age ± standard deviation: 15.96 ± 0.82 years)) were involved in the study, including 62 handball players (28 female and 34 male), 58 volleyball players (30 female and 28 male) and 57 basketball players (29 female and 28 male). In the study, two questionnaires were applied: one implemented to identify the attentive style with the two dimensions internal and external (standardized), called questionnaire for the assessment of attentional style in athletes (QASA), and one designed by us, called questionnaire to identify the weights of attention qualities according to the characteristics of the practiced sport (QAQCS), aiming to identify the most relevant quality of attention, depending on the practiced sport. Cronbach’s alpha for both questionnaires was between 0.701 and 0.855. The results recorded in the present study reveal a variation in the attentional style between the groups of athletes and between genders. The results of the study highlight that in handball, girls have a predominantly external style with a total of 10.213 points; in boys’ handball, the predominant focus of attention is internal with a total score of 9.087 points. Girls’ volleyball focus of attention is predominantly external, with 8.999 points; in boys’ volleyball, the attention style is internal, registering a score of 9.713 points. In girls’ basketball, the predominant focus of attention is internal, with a total score of 8.516 points; in boys’ basketball, the external attention style is predominant, with 9.213 points. Looking at the weight of attention qualities, it was found that the most relevant for girls is concentration and mobility for handball players, stability was identified in volleyball, and distributiveness in basketball. In boys’ handball teams, mobility is the most essential, just like in basketball, and in volleyball it was found that stability has the biggest impact. ANOVA analysis highlighted statistically significant differences between groups of sports games by gender category, at both subscales of (QASA), as well as QAQCS, p < 0.05. The results of our study highlight differences between the attentional styles and their qualities in relation to the gender and the specific sport played, which determines differentiated approaches to these psychological components.
The Mediating and Moderating Role of Social–Emotional Skills in the Relationship between Sports Participation and Test Anxiety
Authors: Wang Kun , Qian Jiali
Published: June 2024

Purpose: Test anxiety is a prevalent issue among adolescents, prompting a need for effective coping mechanisms. Participation in sports, which is gaining recognition for its crucial role in alleviating test anxiety, may be effective due to its association with social–emotional skills. Moreover, students with diverse levels of social–emotional skills not only experience enjoyment in sports differently but also perceive test anxiety uniquely, leading to varying interpretations of the relationships between them. Due to the lack of direct evidence, therefore, this study aimed to explore the intricate relationships among sports participation, test anxiety, and social–emotional skills. Methods: Utilizing OECD data from 61,010 participants across 10 locations, all variable measurements were collected through the Survey on Social and Emotional Skills (SSES). Results: The results indicated that social–emotional skills mediated and moderated the relationship between sports participation and test anxiety. However, only lower- and medium-level social–emotional skills significantly weakened the negative correlation between sports participation and test anxiety. Conclusion: Social–emotional skills serve a dual function in the relationship between sports participation and test anxiety. Not only do they elucidate why sports participation can alleviate test anxiety, but they also act as regulators, moderating the extent of this alleviation. These findings provide valuable insights for educational interventions, underscoring the importance of sports participation and the cultivation of social–emotional skills in mitigating test anxiety.
The Relationship between Organizational Climate and Teaching Innovation among Preschool Teachers: The Mediating Effect of Teaching Efficacy
Authors: Zhang Xianbing , Duan Xiaoshi , Wang Weichen , Qin Jing , Wang Haiying
Published: June 2024

Preschool teachers’ teaching innovation is an important factor in enhancing teaching quality and improving children’s creativity. Based on ecological systems theory and self-determination theory, the purpose of this study was to investigate the relationship between kindergartens’ organizational climate and preschool teachers’ teaching innovation and the mediating role of teaching efficacy in it. In this study, an online questionnaire was distributed to 2092 preschool teachers from different provinces using an Organizational Climate Scale, Teaching Efficacy Scale, and Teaching Innovation Scale. The study used SPSS 25.0 software and the SPSS PROCESS macro program for data processing. The results showed that there was a positive correlation among kindergartens’ organizational climate, teaching efficacy, and teaching innovation, and that kindergartens’ organizational climate not only directly and positively predicted teaching innovation, but also indirectly predicted teaching innovation through the mediating role of teaching efficacy. The study explored the internal and external influences on preschool teachers’ teaching innovation and revealed their underlying mechanisms, providing theoretical support for research and educational practice on preschool teachers’ teaching innovation and children’s creativity.
The Self-Concept and Its Relationship with Parental Socialization and Environment in Primary School Students
Authors: Sánchez-Urrea Antonio , Izquierdo-Rus Tomás , Baena-Morales Salvador , Gómez-Mármol Alberto
Published: June 2024

Self-concept in this article is considered in physical education, with the physical self-concept being in the foreground, and it is analyzed in this quantitative study to determine whether it is related with socio-economic environment and parental styles, focusing on primary education students in the Region of Murcia. Primary education students are in a critical period for the development of self-concept. To assess self-concept levels, socio-economic and cultural environments as well as parental socialization styles, the PSPP, NSE and ESPA29 questionnaires were respectively used. They were used to collect data from 937 students across various educational centers in upper courses (4th, 5th and 6th grades of primary education) to uncover realities in child society and their relationship with self-concept. In the first objective, parental socialization styles are shown to be related with self-concept. However, in the second objective, it is seen that the socio-economic environment is more related with self-concept than parental styles. Finally, the results of the third objective show that there is indeed a relationship with the socio-economic environment and the student body self-concept, both at the family level and for fathers and mothers independently. It is important to consider both the parental styles and the socio-economic environment in which primary education students develop for the development of self-concept. Additionally, educational implications and new lines of research are suggested in this topic.
Design of an Instant Vibration-Based Warning System and Its Operation during Relocation Works of Historic Facades
Authors: Lorenzana Antolin , Villacorta Juan Jose , Magdaleno Alvaro , Val Lara del , Izquierdo Alberto
Published: June 2024

Preserved listed building facades may require large-scale and highly technical work when the supporting building structure is at serious risk of collapse. Such is the case described in this paper, where vast facades must be cut into large panels up to 200 m2 and 150 t in weight and carefully laid on the ground. Various engineering works must be carried out to ensure the structural integrity of the panels to be safeguarded. Each panel must be reinforced by a temporary lattice steel structure prior to the disengagement from the supporting building frame. The operations require the use of cutting tools, hitting demolition machines and heavy cranes, which can induce potentially damaging vibrations that should be monitored and processed so that workers can be alerted in real time if certain thresholds are exceeded so that they can proceed more carefully. The paper describes the specifically designed monitoring system, its electronic parts, how they operate and how the data are processed and displayed. The monitoring system, once verified in laboratory tests, is applied to the detachment and overturning activities of a representative full-scale panel, tracking vibration levels and tilting rates. After days of operation and visual observation, it is possible to correlate vibration levels with incipient damage, establishing that peaks below 0.5 m/s2 or RMS values of 0.05 m/s2 are permissible, but that above 1.0 m/s2 or 0.3 m/s2, respectively, activities should be halted. The proposed system has proven to be useful for the intended purposes, making it possible to know the acceptable thresholds and trigger the necessary alarms in real time for the successful course of the work.
Experimental and Numerical Investigation of a New Type of Composite Slab with a Separate Joint
Authors: Chen Xiulong , Luo Bin , Liu Xin
Published: June 2024

This study proposes a new type of composite slab with a separate joint, which not only improves the assembly rate but also enables unsupported construction. A static loading test was conducted to investigate the bending behavior of the composite slab with a separate joint under a distributed load. The test results showed that the test composite slab had good integrity and crack resistance. The separate joint can effectively transmit internal forces and conform to the requirements of practical engineering. In addition, a finite element model was developed for the composite slab. The experimental load and numerical load corresponding to the longitudinal crack penetrating the bottom surface of the slab were 9.0 kN/m2 and 8.6 kN/m2, respectively, with an error of less than 5%. It was demonstrated that the finite element model can accurately predict the mechanical behavior of the composite slab.
Big Data Adoption in the Chinese Construction Industry: Status Quo, Drivers, Challenges, and Strategies
Authors: Gong Dandan , Zhao Xiaojing , Yang Bohan
Published: June 2024

Under the influence of pervasive digital revolution, the accessibility and analysis of ‘big data’ can provide useful insights and help various industries evolve. Despite the popularity of big data, the construction industry is lagging behind other industries in adopting big data technologies. This paper fills the knowledge gap by examining the status quo of big data adoption in companies with different sizes and roles, as well as that in projects with different types, and ascertaining the drivers for and challenges in adopting big data. This paper employed a structured questionnaire survey and statistical analyses to investigate the significance of factors influencing the drivers, challenges, and enhancement strategies of big data adoption, and validated the results with post-study interviews with construction professionals. The results show that big data adoption in the construction industry is affected by the size of companies and the work experience of their employees. Technology advancement, competitiveness, and government plan and policy initiatives are identified as the top three drivers of big data adoption in the construction sector. Moreover, a lack of appropriate supporting systems, difficulties in data collection, and the shortage of knowledge and experience are found to be the major challenges in big data adoption. Finally, the identified top three strategies for overcoming these challenges and promoting big data adoption are ‘clear organization structure’, ‘government incentives’, and ‘the training of information technology (IT) personnel’. The paper suggests the necessity of creating differentiated strategies for big data adoption for companies with different scales and roles, and helps provide useful insights for policy-makers in promoting big data applications.
Stabilization Effects of Inclined Soil–Cement Continuous Mixing Walls for Existing Warm Frozen Soil Embankments
Authors: Sun Gaochen , Li Long , Huo Yufan , Fang Zonghui , Lv Gao , Chen Tao , Wang Zhengzhong , Song Meimei , Lan Guanqi , Tang Ziqiang
Published: June 2024

Affected by climate warming and anthropogenic disturbances, the thermo-mechanical stability of warm and ice-rich frozen ground along the Qinghai–Tibet Railway (QTR) is continuously decreasing, and melting subsidence damage to existing warm frozen soil (WFS) embankments is constantly occurring, thus seriously affecting the stability and safety of the existing WFS embankments. In this study, in order to solve the problems associated with the melting settlement of existing WFS embankments, a novel reinforcement technology for ground improvement, called an inclined soil–cement continuous mixing wall (ISCW), is proposed to reinforce embankments in warm and ice-rich permafrost regions. A numerical simulation of a finite element model was conducted to study the freeze–thaw process and evaluate the stabilization effects of the ISCW on an existing WFS embankment of the QTR. The numerical investigations revealed that the ISCW can efficiently reduce the melt settlement in the existing WFS embankment, as well as increase the bearing capacity of the existing WFS embankment, making it favorable for improving the bearing ability of composite foundations. The present investigation breaks through the traditional ideas of “active cooling” and “passive protection” and provides valuable guidelines for the choice of engineering supporting techniques to stabilize existing WFS embankments along the QTR.
Quantification and Profiling of Early and Late Differentiation Stage T Cells in Mantle Cell Lymphoma Reveals Immunotherapeutic Targets in Subsets of Patients
Authors: Lokhande Lavanya , Nilsson Daniel , de Matos Rodrigues Joana , Hassan May , Olsson Lina M. , Pyl Paul-Theodor , Vasquez Louella , Porwit Anna , Gerdtsson Anna Sandström , Jerkeman Mats , Ek Sara
Published: June 2024

With the aim to advance the understanding of immune regulation in MCL and to identify targetable T-cell subsets, we set out to combine image analysis and spatial omic technology focused on both early and late differentiation stages of T cells. MCL patient tissue (n = 102) was explored using image analysis and GeoMx spatial omics profiling of 69 proteins and 1812 mRNAs. Tumor cells, T helper (TH) cells and cytotoxic (TC) cells of early (CD57−) and late (CD57+) differentiation stage were analyzed. An image analysis workflow was developed based on fine-tuned Cellpose models for cell segmentation and classification. TC and CD57+ subsets of T cells were enriched in tumor-rich compared to tumor-sparse regions. Tumor-sparse regions had a higher expression of several key immune suppressive proteins, tentatively controlling T-cell expansion in regions close to the tumor. We revealed that T cells in late differentiation stages (CD57+) are enriched among MCL infiltrating T cells and are predictive of an increased expression of immune suppressive markers. CD47, IDO1 and CTLA-4 were identified as potential targets for patients with T-cell-rich MCL TIME, while GITR might be a feasible target for MCL patients with sparse T-cell infiltration. In subgroups of patients with a high degree of CD57+ TC-cell infiltration, several immune checkpoint inhibitors, including TIGIT, PD-L1 and LAG3 were increased, emphasizing the immune-suppressive features of this highly differentiated T-cell subset not previously described in MCL.
Cancer Patients’ Perspectives and Requirements of Digital Health Technologies: A Scoping Literature Review
Authors: Lazarou Ioulietta , Krooupa Anna-Maria , Nikolopoulos Spiros , Apostolidis Lazaros , Sarris Nikos , Papadopoulos Symeon , Kompatsiaris Ioannis
Published: June 2024

Digital health technologies have the potential to alleviate the increasing cancer burden. Incorporating patients’ perspectives on digital health tools has been identified as a critical determinant for their successful uptake in cancer care. The main objective of this scoping review was to provide an overview of the existing evidence on cancer patients’ perspectives and requirements for patient-facing digital health technologies. Three databases (CINAHL, MEDLINE, Science Direct) were searched and 128 studies were identified as eligible for inclusion. Web-based software/platforms, mobile or smartphone devices/applications, and remote sensing/wearable technologies employed for the delivery of interventions and patient monitoring were the most frequently employed technologies in cancer care. The abilities of digital tools to enable care management, user-friendliness, and facilitate patient–clinician interactions were the technological requirements predominantly considered as important by cancer patients. The findings from this review provide evidence that could inform future research on technology-associated parameters influencing cancer patients’ decisions regarding the uptake and adoption of patient-facing digital health technologies.
Green Synthesis of Silver Nanoparticles from Cannabis sativa: Properties, Synthesis, Mechanistic Aspects, and Applications
Authors: Ahmadi Fatemeh , Lackner Maximilian
Published: June 2024

The increasing global focus on green nanotechnology research has spurred the development of environmentally and biologically safe applications for various nanomaterials. Nanotechnology involves crafting diverse nanoparticles in terms of shapes and sizes, with a particular emphasis on environmentally friendly synthesis routes. Among these, biogenic approaches, including plant-based synthesis, are favored for their safety, simplicity, and sustainability. Silver nanoparticles, in particular, have garnered significant attention due to their exceptional effectiveness, biocompatibility, and eco-friendliness. Cannabis (Cannabis sativa L.) has emerged as a promising candidate for aiding in the green synthesis of silver nanoparticles. Leveraging the phytochemical constituents of Cannabis, researchers have successfully tailored silver nanoparticles for a wide array of applications, spanning from biomedicine to environmental remediation. This review explores the properties, synthesis mechanisms, and applications of silver nanoparticles obtained from Cannabis. Additionally, it delves into the recent advancements in green synthesis techniques and elucidates the optical properties of these nanoparticles. By shedding light on plant-based fabrication methods for silver nanoparticles and their diverse bionanotechnology applications, this review aims to contribute to the growing body of knowledge in the field of green nanotechnology. Through a comprehensive examination of the synthesis processes, mechanistic aspects, and potential applications, this review underscores the importance of sustainable approaches in nanoparticle synthesis and highlights the potential of Cannabis-derived silver nanoparticles in addressing various societal and environmental challenges.
Highly Efficient Visible-Light-Driven Photocatalysis of Rose Bengal Dye and Hydrogen Production Using Ag@Cu/TiO2 Ternary Nanocomposites
Authors: Yadav Satish , Jilani Asim , Sachan Sarika , Kumar Pramod , Ansari Sajid Ali , Afzal Muhammad , Ansari Mohammad Omaish
Published: June 2024

In this work, an Ag@Cu/TiO2 ternary nanocomposite was synthesized by a simple chemical methodology and subsequently studied for the photocatalytic degradation of rose bengal (RB) dye under visible light as well as its hydrogen production. The shape, size and topographical analysis by scanning and transmission electron microscopy revealed that all the constituents are well intercalated and are in the nano range. The energy dispersive X-ray analysis of the Ag@Cu/TiO2 showed the presence of Ti, O, Cu and Ag and the absence of any other impurities, while the mapping analysis showed their uniform distribution. The X-ray photon spectroscopy also showed successful interaction between the components. Furthermore, the changes in the chemical state of Ti2p were examined. The band gap of Ag@Cu/TiO2 using the Tauc plot relations was found to be the lowest at 2.86 eV in comparison to pure TiO2 (3.28 eV), binary Ag/TiO2 (3.13 eV) and Cu/TiO2 (3.00 eV). The Ag@Cu/TiO2 displayed the lowest photoluminescence intensity, suggesting the highest degradation efficiency and lowest recombination rate. The application of Ag@Cu/TiO2 toward the photocatalytic degradation of RB dye exhibited a degradation rate of ~81.07%, which exceeds the efficiency of pure TiO2 by 3.31 times. Apart from this, the hydrogen production of Ag@Cu/TiO2 was found to be 17.1 μmol h−1 g−1, suggesting that copper and silver synergistically contributed, thereby resulting in the increased hydrogen production of pure TiO2.
Role of Brain Elastography in the Neonatal Setting: State of the Art of Ultrasonographic Techniques and Future Perspectives
Authors: Piersigilli Fiammetta , Campi Francesca , Savarese Immacolata , Iacona Giulia , Auriti Cinzia , Dotta Andrea , Braguglia Annabella , Garcovich Matteo , Bersani Iliana
Published: June 2024

Magnetic resonance imaging is currently used in the neonatal setting for assessing features of the neonatal brain. However, its utilization is constrained by logistic, technical, or clinical challenges. Brain elastography is a new research technique which enhances the diagnostic capability of traditional imaging, and can be paired with both ultrasonography and magnetic resonance imaging. In particular, brain elastography adds objective and quantitative information to traditional imaging by detecting differences in tissue elasticity/stiffness, which may represent a surrogate marker of the physiologic and pathologic features of the neonatal brain. To date, very limited experience exists about the use of brain elastography specifically in the neonatal setting. The aim of the present review was to describe the most recent information about the feasibility and diagnostic accuracy of brain ultrasound elastography (USE) in neonates, and to provide information about the possible future applications and perspectives of brain elastography.
Macrodactyly
Authors: Giżewska-Kacprzak Kaja , Śliwiński Maximilian , Nicieja Karol , Babiak-Choroszczak Lidia , Walaszek Ireneusz
Published: June 2024

Macrodactyly is a rare congenital limb difference manifesting as an overgrowth of one or more fingers or toes. The pathological process affects all tissues of the ray in the hand or foot. The enlargement can significantly alter the limb’s appearance and impair its function. The role of a pediatrician is to distinguish isolated macrodactyly from syndromic conditions (including PIK3CA-Related Overgrowth Spectrum) or mimicking conditions to enable early interdisciplinary consultation and treatment planning. The psychological stigma associated with this often disfiguring condition necessitates support for patients and their family. We present a practical guide for physicians who might be the first to raise suspicion of macrodactyly and initiate further diagnostics to achieve adequate treatment and support for children and caregivers.