bluuebunny/crossref_metadata_2025 huggingface dataset:
Statistical Analysis of the Interdependence between the Technical and Functional Parameters of Electric Vehicles in the European Market
Authors: Mariasiu Florin , Chereches Ioan , Raboca Horia
Published: March 2023

The vehicle market at the European level (and also elsewhere) has registered a high dynamic for the adoption of electric vehicles as the future means of transport. Government policies and decisions fully support this move, but there are still massive barriers to entry into the EV market due to consumer attitudes and perceptions. Consumer attitudes and perceptions will be decisive in the market success of future electric vehicle models, given that consumers are particularly interested in the vehicles’ technical and dynamic performance. The pressure from customers’ demands for performance leaves its mark not only on the increase of performance and technical parameters, but directly contributes to the generation of interdependence between these parameters. This article presents a comparative statistical analysis of 203 electric vehicle models (from various construction categories), present in the European vehicle market (between the years 2019 and 2022), to highlight the direct and indirect links (interdependencies) between technical and performance parameters depending on the constructive class (type). The goal of this article is to determine whether there is a relationship between the three key performance metrics for electric vehicles—autonomy, top speed, and acceleration—and five significant technical/constructive metrics for these vehicles—battery capacity, energy efficiency, electric motor power, fast charging speed, and vehicle weight (brand and year of availability in the market). Based on the analyzed data, it can be stated that the design and construction of an electric vehicle model currently takes into account both performance and technical parameters, with a strong link between the weight of the vehicle and the energy capacity of the battery (+0.687) being highlighted and also the relationship between autonomy and vehicle weight (+0.355). The conclusions of this study can be used in the future by manufacturers for the development of new models of electric vehicles (new generic platforms and chassis) by classifying/standardizing these vehicles into specific classes, corresponding to the requirements of different classes of consumers or identifying constructive solutions specific to each type of consumer.
Manufacturing and Testing the Panels with a Transverse Texture Obtained from Branches of Norway Spruce (Picea abies L. Karst.)
Authors: Olarescu Alin M. , Lunguleasa Aurel , Radulescu Loredana , Spirchez Cosmin
Published: March 2023

As a result of the imbalances in the forestry market and the increased demand for wood products worldwide, the resource of branches resulting from the exploitation of a forest has attracted special attention from researchers, in order to use these secondary resources judiciously and obtain an added value superior to classic uses. In this context, the current research took into consideration the use of spruce branches to obtain panels with a transverse structure. The work methodology has focused on the process of obtaining panels with a transverse texture and on determining the physical–mechanical properties of the created panels. The results regarding the panel density (determined as a ratio between mass and volume of specimens) showed about 693 kg/m3, static bending resistance parallel to the face of 5.5 N/mm2, resistance of adhesion of 5.6 N/mm2, shear strength parallel to face of 4.1 N/mm2, and screw pull-out resistance perpendicular to the face of 31.3 N/mm2, highlighting that the properties were in accordance with the European standards and that the panels obtained were suitable for obtaining furniture products with a special aesthetic aspect. As a general conclusion of the research, it can be stated that spruce branches are a sustainable wood resource with great possibilities to add more value in the form of panels with a traverse texture.
Possibilities of Using UAV for Estimating Earthwork Volumes during Process of Repairing a Small-Scale Forest Road, Case Study from Kyoto Prefecture, Japan
Authors: Hasegawa Hisashi , Sujaswara Azwar Azmillah , Kanemoto Taisei , Tsubota Kazuya
Published: March 2023

Although forest road networks are an important infrastructure for forestry, recreation, and sustainable forest management, they have a considerable effect on the environment. Therefore, a detailed analysis of the various benefits and associated costs of road network construction is needed. The cost of earthwork in road construction can be estimated based on the change in topography before and after construction. However, accurate estimation of the earthwork volume may not be possible on steep terrain where soil placement is limited. In this study, an unmanned aerial vehicle was flown under the tree canopy six times during a road repair work to measure the changes in topography using structure from motion analysis. Comparing the obtained 3D model with the measurement results from the total station, the average vertical error and root mean square error were −0.146 m and 0.098 m, respectively, suggesting its good accuracy for measuring an earthwork volume. Compared to the amount of earthwork estimated from the topographic changes before and after the repair work, the actual earthwork volume was 3.5 times greater for cutting and 1.9 times greater for filling. This method can be used to calculate the earthwork volume accurately for designing forest road networks on steep terrain.
Effects of Starvation on the Physiology and Liver Transcriptome of Yellowcheek (Elopichthys bambusa)
Authors: Xie Min , Li Shaoming , Feng Zhifeng , Xiang Jin , Deng Qi , Wang Pengpeng , Wu Hao , Gao Jingwei , Zeng Guoqing , Xiang Guangqing
Published: March 2023

Anthropogenic and extreme climate disasters cause ecological changes in natural rivers and lakes, increasing the risk of starvation in yellowcheek (Elopichthys bambusa). Therefore, the impact of starvation on the metabolism and wild population of yellowcheek should be explored. In this study, we used transcriptome sequencing technology to analyze the effects of short (8 d) and long-term (28 d) starvation on the liver transcriptome, growth, and serum indicators of yellowcheek. Our results showed that short-term starvation significantly reduced the visceral weight and viscera index of yellowcheek. Long-term starvation significantly reduced the body weight and Fulton’s condition factor, and it maintained significant reductions in visceral weight and viscera index. These results indicate that glycogen is the preferred energy source, rather than muscle protein, under starvation. Short-term starvation limited N-glycan and fatty acid biosynthesis, fatty acid elongation in the endoplasmic reticulum in the liver, and upregulated fatty acid degradation. However, long-term starvation alleviated the reduction in N-glycan and fatty acid biosynthesis caused by early starvation, and it significantly reduced fatty acid elongation in the mitochondria, as well as fatty acid degradation. These results provide important experiment information for assessing the starvation levels and nutritional status of wild yellowcheek.
Improving the Yield of Feruloyl Oligosaccharides from Rice Bran through Enzymatic Extrusion and Its Mechanism
Authors: Deng Fenghong , Hu Xiuting , Wang Yueru , Luo Shunjing , Liu Chengmei
Published: March 2023

Rice bran, rich in feruloyl arabinoxylan, is a good source of feruloyl oligosaccharides (FOs). To prepare FOs, bran was often hydrolyzed by amylase and protease to remove starch and protein and then hydrolyzed by xylanase, which was time-consuming and had a low yield. To solve the above problems, enzymatic extrusion was used to treat rice bran, and the effects of traditional hydrolysis, a combination of traditional extrusion and hydrolysis (extrusion-hydrolysis) and enzymatic extrusion on the yield of FOs were investigated and compared in this study. It was found that traditional extrusion and enzymatic extrusion significantly increased the yield of FOs. Particularly, the yield of FOs resulting from enzymatic extrusion was increased to 5.78%, while the yield from traditional hydrolysis was 4.23%. Microscopy analysis showed that extrusion damaged the cell wall of bran, which might increase the accessibility of xylanase to arabinoxylan and the yield of FOs. Spectroscopy analysis suggested that FOs obtained by different pretreatments had similar structures. It was obvious that enzymatic extrusion saved the time for removal of starch and protein and increased the yield of FOs. In addition, the highest yield of FOs was found at the moisture content of 30% and the screw speed of 50 rpm. This study provided an efficient method for the preparation of FOs that is suitable for industrial production.
Siraitia grosvenorii (Swingle) C. Jeffrey: Research Progress of Its Active Components, Pharmacological Effects, and Extraction Methods
Authors: Duan Jiajing , Zhu Dong , Zheng Xiuxia , Ju Yang , Wang Fengzhong , Sun Yufeng , Fan Bei
Published: March 2023

Siraitia grosvenorii (Swingle) C. Jeffrey, a perennial vine of the Cucurbitaceae family, is a unique medicine food homology species from China. S. grosvenorii can be used as a natural sweetener in the food industry and as a traditional medicine for moistening the lungs, quenching a cough, smoothing the intestines, and relieving constipation. Additionally, the fruits, roots, stems, and leaves of S. grosvenorii are rich in active ingredients, and have pharmacological effects such as immune regulation, hypoglycemia, and antioxidant, hepatoprotective, and antitumor effects, etc. Therefore, S. grosvenorii has broad application prospects in the pharmaceutical industry. This paper reviews the bioactive components, pharmacological effects, and extraction methods of S. grosvenorii, summarizes them, and proposes their future development directions. This current overview highlights the value of S. grosvenorii. By documenting the comprehensive information of S. grosvenorii, the review aims to provide the appropriate guidelines for its future in-depth development and the utilization of S. grosvenorii resources for their roles as active ingredient (triterpenoids, flavonoids, and polysaccharides, etc.) sources in the food industry and in the development of functional foods.
Combined Effect of Acid Whey Addition and Ultrasonic Treatment on the Chemical and Microbiological Stability of Lamb Stuffing
Authors: Latoch Agnieszka , Stasiak Dariusz M. , Junkuszew Andrzej
Published: March 2023

The microbiological and chemical stability of stuffing is crucial in meat processing. Small ruminant (lamb) meat has many nutritional advantages (fatty acid composition and ratio, high biological value of protein, source of zinc, and selenium) but is poorly utilized in processing. In this experiment, we determined the effects of physical (sonication), chemical (salt, curing salt, and air access), and microbiological (acid whey) factors on the microbiological and chemical stability of lamb meat stuffing. Proximate composition and fatty acids profile, pH, water activity, lipid oxidation, color parameters, and microbiology examinations were performed in fresh meat stuffing and on the fifth day of refrigerated storage. Ultrasound treatment of the meat with whey reduced its acidity and increased the oxidative stability of fats but did not modify the water activity and color of the stuffing. Stuffing sonication did not affect the growth of LAB but reduced the number of Entereobacteriace, especially in the presence of whey and salt. The treatment of lamb meat stuffing with low-frequency and medium-intensity ultrasound assisted by the addition of acid whey and salt is a technique conducive to reducing the use of nitrates in meat technology and their consumption by consumers.
A Comparative Study of Acute Alcoholic Hepatitis vs. Non-Alcoholic Hepatitis Patients from a Cohort with Chronic Alcohol Dependence
Authors: Tun Kyaw Min , Dossaji Zahra , Massey Blaine L. , Batra Kavita , Lo Chun-Han , Naga Yassin , Mohammed Salman , Muraga Abebe , Gill Ahmad , Mukhopadhyay Dwaipayan , Singh Ashok , Lankarani Daisy , Aponte-Pieras Jose , Ohning Gordon
Published: March 2023

The rate of alcoholic hepatitis (AH) has risen in recent years. AH can cause as much as 40–50% mortality in severe cases. Successful abstinence has been the only therapy associated with long-term survival in patients with AH. Thus, it is crucial to be able to identify at-risk individuals in order to implement preventative measures. From the patient database, adult patients (age 18 and above) with AH were identified using the ICD-10 classification from November 2017 to October 2019. Liver biopsies are not routinely performed at our institution. Therefore, patients were diagnosed with AH based on clinical parameters and were divided into “probable” and “possible” AH. Logistic regression analysis was performed to determine risk factors associated with AH. A sub-analysis was performed to determine variables associated with mortality in AH patients. Among the 192 patients with alcohol dependence, there were 100 patients with AH and 92 patients without AH. The mean age was 49.3 years in the AH cohort, compared to 54.5 years in the non-AH cohort. Binge drinking (OR 2.698; 95% CI 1.079, 6.745; p = 0.03), heavy drinking (OR 3.169; 95% CI 1.348, 7.452; p = 0.01), and the presence of cirrhosis (OR 3.392; 95% CI 1.306, 8.811; p = 0.01) were identified as characteristics more commonly found in the AH cohort. Further, a higher inpatient mortality was seen in those with a probable AH diagnosis (OR 6.79; 95% CI 1.38, 44.9; p = 0.03) and hypertension (OR 6.51; 95% CI 9.49, 35.7; p = 0.02). A higher incidence of mortality was also noted among the non-Caucasian race (OR 2.72; 95% CI 4.92; 22.3; p = 0.29). A higher mortality rate despite a lower incidence of alcohol use among non-Caucasian patients may indicate healthcare disparities.
Quantitative Long-Term Monitoring (1890–2020) of Morphodynamic and Land-Cover Changes of a LIA Lateral Moraine Section
Authors: Altmann Moritz , Ramskogler Katharina , Mikolka-Flöry Sebastian , Pfeiffer Madlene , Haas Florian , Heckmann Tobias , Rom Jakob , Fleischer Fabian , Himmelstoß Toni , Pfeifer Norbert , Ressl Camillo , Tasser Erich , Becht Michael
Published: March 2023

Aerial photographs of the European Alps usually only reach back to the middle of the 20th century, which limits the time span of corresponding studies that quantitatively analyse long-term surface changes of proglacial areas using georeferenced orthophotos. To the end of the Little Ice Age, this leads to a gap of about 100 years. Using digital monoplotting and several historical terrestrial photographs, we show the quantification of surface changes of a Little Ice Age lateral moraine section until the late second half of the 19th century, reaching a total study period of 130 years (1890–2020). The (initial) gully system expands (almost) continuously over the entire study period from 1890 to 2020. Until 1953, the vegetation-covered areas also expanded (mainly scree communities, alpine grasslands and dwarf shrub communities), before decreasing again, especially between 1990 and 2003, due to large-scale erosion within the gully system. Furthermore, our results show that the land-cover development was impacted by temperature and precipitation changes. With the 130-year study period, we contribute to a substantial improvement in the understanding of the processes in the proglacial by analysing the early phase and thus the immediate response of the lateral moraine to the ice exposure.
Clinical Characterization and Outcomes of Patients with Hypercreatinemia Affected by COVID-19
Authors: Elkhalifa Ahmed M. E. , Shah Naveed Nazir , Khan Zaid , Ali Sofi Imtiyaz , Nabi Showkat Ul , Bashir Showkeen Muzamil , Mir Masood Saleem , Bazie Elsharif. A. , Elderdery Abozer Y. , Alanazi Awadh , Alenazy Fawaz O. , Ahmed Elsadig Mohamed
Published: March 2023

The present study evaluated the clinical presentation and outcome of COVID-19 patients with underlying hypercreatinemia at the time of hospitalization. A retrospective observational study was conducted from the 23rd of March 2020 to the 15th of April 2021 in 1668 patients confirmed positive for COVID-19 in the Chest Disease Hospital in Srinagar, India. The results of the present study revealed that out of 1668 patients, 339 with hypercreatinemia had significantly higher rates of admission to the intensive care unit (ICU), severe manifestations of the disease, need for mechanical ventilation, and all-cause mortality. Multivariable analysis revealed that age, elevated creatinine concentrations, IL-1, D-Dimer, and Hs-Crp were independent risk factors for in-hospital mortality. After adjusted analysis, the association of creatinine levels remained strongly predictive of all-cause, in-hospital mortality (HR-5.34; CI-4.89–8.17; p ≤ 0.001). The amelioration of kidney function may be an effective method for achieving creatinemic targets and, henceforth, might be beneficial for improving outcomes in patients with COVID-19.
Landscape and Agriculture 4.0: A Deep Farm in Italy in the Underground of a Public Historical Garden
Authors: Devecchi Marco , Ghersi Adriana , Pilo Andrea , Nicola Silvana
Published: March 2023

Each landscape is the result of an encounter with the culture of a community and the physical features of a territory. The conservation of the historical, artistic, and cultural heritage represents a priority for any society that wishes to draw on references for its civil progress. The aim of the present research is to combine the richness of the historical–cultural heritage with innovative forms of agriculture. It focuses on the recovery, in productive terms, of an air-raid shelter used during the Second World War, located in the center of Varese beneath the Estensi Historical Gardens. The project involves the construction of an underground Vertical Farm (Deep Farm) with the aim of restoring a place of memory, making it more accessible than it is today, and raising public awareness about a new cultivation model. A Deep Farm was designed with a cultivation area in the middle, an educational room, and two hygiene rooms, one at each end of the tunnel. A Vertical Farm was conceived to be shared with local stakeholders to produce vegetables and to foresee an innovative reality in the field of education and tourism. This project has the ambition of representing a model that could be used for similar Italian realities and enhancing meeting places between landscape and modern culture diversities.
Anti-Cholinesterase and Anti-α-Amylase Activities and Neuroprotective Effects of Carvacrol and p-Cymene and Their Effects on Hydrogen Peroxide Induced Stress in SH-SY5Y Cells
Authors: Caputo Lucia , Amato Giuseppe , De Martino Laura , De Feo Vincenzo , Nazzaro Filomena
Published: March 2023

Several researchers have demonstrated the health and pharmacological properties of carvacrol and p-cymene, monoterpenes of aromatic plants. This study investigated these compounds’ possible anti-cholinesterase, anti-α-amylase, and neuroprotective effects. We evaluated the anti-acetylcholinesterase and anti-α-amylase activities at different concentrations of the compounds. The maximum non-toxic dose of carvacrol and p-cymene against SH-SY5Y neuroblastoma cells was determined using an MTT assay. The neuroprotective effects of the compounds were evaluated on H2O2-induced stress in SH-SY5Y cells, studying the expression of caspase-3 using Western blotting assays. Carvacrol showed inhibitory activities against acetylcholinesterase (IC50 = 3.8 µg/mL) and butyrylcholinesterase (IC50 = 32.7 µg/mL). Instead, the anti-α-amylase activity of carvacrol resulted in an IC50 value of 171.2 μg/mL After a pre-treatment with the maximum non-toxic dose of carvacrol and p-cymene, the expression of caspase-3 was reduced compared to cells treated with H2O2 alone. Carvacrol and p-cymene showed in vitro anti-enzymatic properties, and may act as neuroprotective agents against oxidative stress. Further studies are necessary to elucidate their possible use as coadjutants in preventing and treating AD in diabetic patients.
Melanoma Cells Produce Large Vesicular-Bodies That Cause Rapid Disruption of Brain Endothelial Barrier-Integrity and Disassembly of Junctional Proteins
Authors: Spurling Dayna , Anchan Akshata , Hucklesby James , Finlay Graeme , Angel Catherine E. , Graham E. Scott
Published: March 2023

It is known that many cells produce extracellular vesicles, and this includes a range of different cancer cell types. Here we demonstrate the profound effects of large vesicular-like bodies produced by melanoma cells on the barrier integrity of human brain endothelial cells. These vesicular-bodies have not been fully characterised but range in size from ~500 nm to >10 µm, are surrounded by membrane and are enzymatically active based on cell-tracker incorporation. Their size is consistent with previously reported large oncosomes and apoptotic bodies. We demonstrate that these melanoma-derived vesicular-bodies rapidly affect brain endothelial barrier integrity, measured using ECIS biosensor technology, where the disruption is evident within ~60 min. This disruption involves acquisition of the vesicles through transcellular uptake into the endothelial cells. We also observed extensive actin-rearrangement, actin removal from the paracellular boundary of the endothelial cells and envelopment of the vesicular-bodies by actin. This was concordant with widespread changes in CD144 localisation, which was consistent with the loss of junctional strength. High-resolution confocal imaging revealed proximity of the melanoma vesicular-bodies juxtaposed to the endothelial nucleus, often containing fragmented DNA themselves, raising speculation over this association and potential delivery of nuclear material into the brain endothelial cells. The disruption of the endothelial cells occurs in a manner that is faster and completely distinct to that of invasion by intact melanoma cells. Given the clinical observation of large vesicles in the circulation of melanoma patients by others, we hypothesize their involvement in weakening or priming the brain vasculature for melanoma invasion.
Improved Real-Time Quaking Induced Conversion for Early Diagnostics of Creutzfeldt–Jakob Disease in Denmark
Authors: Bsoul Remarh , Lund Eva Løbner , Burns Kimberley , Andrews Mary , McKenzie Neil , Green Alison , Areškevičiūtė Aušrinė
Published: March 2023

Cerebrospinal fluid-based real-time quaking-induced conversion (CSF RT-QuIC) is currently the most prominent method for early detection of sporadic Creutzfeldt–Jakob disease (sCJD), the most common prion disease. CSF RT-QuIC delivers high sensitivity (>90%) and specificity (100%), which has been demonstrated by large ring-trial studies testing probable and definitive sCJD cohorts. Following the inclusion of CSF RT-QuIC in the revised European CJD Surveillance Network diagnostic criteria for sCJD, it has become a standard diagnostic procedure in many prion disease reference or surveillance centers around the world. In this study, we present the implementation of the second-generation CSF RT-QuIC (commonly known as Improved QuIC or IQ) at the Danish Reference Center for Prion Diseases (DRCPD). The method’s sensitivity and specificity were evaluated and validated by analyzing 63 CSF samples. These 63 samples were also analyzed at the National CJD Research and Surveillance Unit (NCJDRSU), based at the University of Edinburgh, UK; analysis was carried out using the first generation or previous CSF RT-QuIC method (PQ). The sensitivity and specificity of PQ during tests at the NCJDRSU were 92% and 100%, respectively. Using these 63 CSF samples, the agreement between the two RT-QuIC generations at DRCPD and NCJDRSU prion laboratories was 100%.
Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in Drosophila
Authors: Zhao Tujing , Wang Min , Li Zheng , Li Hao , Yuan Dongqin , Zhang Xing , Guo Mengge , Qian Wenliang , Cheng Daojun
Published: March 2023

Lipid homeostasis is essential for insect growth and development. The complex of proteins associated with Set 1 (COMPASS)-catalyzed Histone 3 lysine 4 trimethylation (H3K4me3) epigenetically activates gene transcription and is involved in various biological processes, but the role and molecular mechanism of H3K4me3 modification in lipid homeostasis remains largely unknown. In the present study, we showed in Drosophila that fat body-specific knockdown of will die slowly (Wds) as one of the COMPASS complex components caused a decrease in lipid droplet (LD) size and triglyceride (TG) levels. Mechanistically, Wds-mediated H3K4me3 modification in the fat body targeted several lipogenic genes involved in lipid synthesis and the Lpp gene associated with lipid transport to promote their expressions; the transcription factor heat shock factor (Hsf) could interact with Wds to modulate H3K4me3 modification within the promoters of these targets; and fat body-specific knockdown of Hsf phenocopied the effects of Wds knockdown on lipid homeostasis in the fat body. Moreover, fat body-specific knockdown of Wds or Hsf reduced high-fat diet (HFD)-induced oversized LDs and high TG levels. Altogether, our study reveals that Wds-mediated H3K4me3 modification is required for lipid homeostasis during Drosophila development and provides novel insights into the epigenetic regulation of insect lipid metabolism.
A Standardized Protocol for Assuring the Validity of Proteomics Results from Liquid Chromatography–High-Resolution Mass Spectrometry
Authors: Gawor Andrzej , Bulska Ewa
Published: March 2023

Significant advances in the technological development of mass spectrometry in the field of proteomics and the generation of extremely large amounts of data require a very critical approach to assure the validity of results. Commonly used procedures involved liquid chromatography followed by high-resolution mass spectrometry measurements. Proteomics analysis is used in many fields including the investigation of the metabolism of biologically active substances in organisms. Thus, there is a need to care about the validity of the obtained results. In this work, we proposed a standardized protocol for proteomic analysis using liquid chromatography–high-resolution mass spectrometry, which covers all of these analytical steps to ensure the validity of the results. For this purpose, we explored the requirements of the ISO/IEC 17025:2017 standard as a reference document for quality control in biochemistry research-based mass spectrometry.
Low P-Selectin Glycoprotein Ligand-1 Expression in Neutrophils Associates with Disease Activity and Deregulated NET Formation in Systemic Lupus Erythematosus
Authors: Muñoz-Callejas Antonio , González-Sánchez Elena , Silván Javier , San Antonio Esther , González-Tajuelo Rafael , Ramos-Manzano Alejandra , Sánchez-Abad Inés , González-Alvaro Isidoro , García-Pérez Javier , Tomero Eva G. , de Vicuña Rosario García , Vicente-Rabaneda Esther F. , Castañeda Santos , Urzainqui Ana
Published: March 2023

Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the generation of anti-DNA autoantibodies due to exposure of immune cells to excessive amounts of extracellular DNA. Lack of P-selectin in mice induces the development of a lupus-like syndrome and patients with cutaneous lupus have reduced P-selectin expression in skin vessels. Using flow cytometry we analyzed in healthy donors and patients the expression of P-selectin Glycoprotein Ligand-1 (PSGL-1) in circulating neutrophils and the implication of PSGL-1/P-selectin interaction in neutrophil extracellular traps (NETs) generation. We found a statistical significance that neutrophils from active SLE patients have a reduced expression of PSGL-1 and low levels of PSGL-1 in neutrophils from SLE patients associated with the presence of anti-dsDNA antibodies, clinical lung involvement, Raynaud’s phenomenon, and positive lupus anticoagulant. PSGL-1 is present along the DNA in the NET. In healthy donors, neutrophil interaction with immobilized P-selectin triggers Syk activation, increases the NETs percentage and reduces the amount of DNA extruded in the NETs. In active SLE patients, neutrophil interaction with P-selectin does not activate Syk or reduce the amount of DNA extruded in the NETs, that might contribute to increase the extracellular level of DNA and hence, to disease pathogenesis.
Calibration of Micromechanical Parameters for the Discrete Element Simulation of a Masonry Arch using Artificial Intelligence
Authors: Kibriya Ghulam , Orosz Ákos , Botzheim János , Bagi Katalin
Published: March 2023

This study focuses on an old but still unresolved problem of automatically calibrating the constitutive parameters of discrete element models. Instead of the troublesome and time-consuming manual trial-and-error method, which is typical today, the authors suggest using artificial intelligence techniques. A masonry arch is analysed, whose experimental static load–displacement behaviour is known from the literature. An attempt is made to match this behaviour with discrete element models, through finding appropriate quantitative values for the parameters. Two methods (Genetic Algorithm (GA) and Particle Swarm Optimisation (PSO)) are tested and, since PSO turns out to be more reliable, a further improved version, ‘Trust-Based Particle Swarm Optimisation’ (TBPSO), is proposed. The results show that (1) TBPSO quickly leads to suitable alternative parameter sets that make the discrete element model match the behaviour of the real experiments and (2) the optimal values of the parameters strongly depend on the loading velocity and the discretisation method used.
Comparative Spectroscopic and Electrochemical Study of V(V)-Substituted Keggin-Type Phosphomolybdates and -Tungstates
Authors: Raabe Jan-Christian , Aceituno Cruz José , Albert Jakob , Poller Maximilian J.
Published: March 2023

Vanadium-substituted Keggin-type heteropolyanions have been studied for a wide variety of applications, ranging from catalysis to antiviral/antimicrobial agents. While the V-substituted phosphomolybdates [PVxMo12−xO40](3+x)− have been well investigated in this context, comparatively little is known about the corresponding phosphotungstates [PVxW12-xO40](3+x)−. We have succeeded in synthesizing the sodium salts of the whole series [PVxW12−xO40](3+x)−, for x = 1 to 6, and characterised them spectroscopically (FT-IR, UV-Vis, 31P-, and 51V-NMR) and electrochemically (CV and SWV). Thereby, direct comparisons between the vanadium-substituted phosphomolybdates and -tungstates, with substitution degrees from 1 to 6, can be established, which provides a solid basis for further investigations of potential applications.
Analysis of the Composition of Different Instars of Tenebrio molitor Larvae using Near-Infrared Reflectance Spectroscopy for Prediction of Amino and Fatty Acid Content
Authors: Kröncke Nina , Wittke Stefan , Steinmann Nico , Benning Rainer
Published: March 2023

Insects are a sustainable protein source for food and feed. The yellow mealworm (Tenebrio molitor L.) is a promising candidate for industrial insect rearing and was the focus of this study. This research revealed the diversity of Tenebrio molitor larvae in the varying larval instars in terms of the nutritional content. We hypothesized that water and protein are highest in the earlier instar, while fat content is very low but increases with larval development. Consequently, an earlier instar would be a good choice for harvest, since proteins and amino acids content decrease with larval development. Near-infrared reflectance spectroscopy (NIRS) was represented in this research as a tool for predicting the amino and fatty acid composition of mealworm larvae. Samples were scanned with a near-infrared spectrometer using wavelengths from 1100 to 2100 nm. The calibration for the prediction was developed with modified partial least squares (PLS) as the regression method. The coefficient for determining calibration (R2C) and prediction (R2P) were >0.82 and >0.86, with RPD values of >2.20 for 10 amino acids, resulting in a high prediction accuracy. The PLS models for glutamic acid, leucine, lysine and valine have to be improved. The prediction of six fatty acids was also possible with the coefficient of the determination of calibration (R2C) and prediction (R2P) > 0.77 and >0.66 with RPD values > 1.73. Only the prediction accuracy of palmitic acid was very weak, which was probably due to the narrow variation range. NIRS could help insect producers to analyze the nutritional composition of Tenebrio molitor larvae fast and easily in order to improve the larval feeding and composition for industrial mass rearing.
Assessing the Larvicidal Properties of Endemic Campeche, Mexico Plant Piper cordoncillo var. apazoteanum (Piperaceae) against Aedes aegypti (Diptera: Culicidae) Mosquitoes
Authors: Alonso-Hernández Nancy , Granados-Echegoyen Carlos , Vera-Reyes Ileana , Pérez-Pacheco Rafael , Arroyo-Balán Fabián , Valdez-Calderón Alejandro , Espinosa-Roa Arián , Loeza-Concha Henry Jesús , Villanueva-Sánchez Evert , García-Pérez Florinda , Diego-Nava Fidel
Published: March 2023

The research aims to investigate the mortality effect of essential oil from Piper cordoncillo var. apazoteanum, an endemic plant from Campeche, Mexico, on early second-instar Aedes aegypti larvae; it also aims to identify the volatile compounds present in the fresh leaves of the plant. To test the effectiveness of the essential oil, we followed World Health Organization Standard Procedures. Larvae were observed for 17 consecutive days after treatment to determine the mortality and growth-inhibitory effect exerted by the essential oil. The results showed that the essential oil was effective in controlling mosquito populations. At a concentration of 800 ppm, the oil achieved an effectiveness rate of 70.00 ± 8.16% after 24 h, increasing to 100.00 ± 0.01% mortality after 72 h. With a concentration of 400 ppm, the effectiveness was 98.33 ± 0.17% by the end of the experiment. Furthermore, the obtained results demonstrated that the LC50 value was 61.84 ± 6.79 ppm, while the LC90 value was 167.20 ± 11.49 ppm. Essential oil concentrations inhibited the growth of immature insect stages, with concentrations between 800–100 ppm demonstrating very high inhibitory activity, and the lowest concentration of 50 ppm showing high inhibitory activity. The study also identified 24 chemical compounds representing 86.71% of the volatile compound composition of the fresh leaves of P. cordoncillo; the most abundant compounds were Safrole, Caryophyllene oxide, E-Nerolidol, and Calarene epoxide. The method used to extract the volatile compounds, solvent-free microwave extraction (SFME), is a promising alternative to traditional methods that avoids the use of potentially harmful solvents, making it more ecologically friendly and potentially safer for professionals handling the extracted compounds. Overall, the study demonstrates the potential of P. cordoncillo essential oil as an effective means of controlling mosquito populations, and provides valuable information on the chemical composition of the plant.Moreover, our study is the first to report on the biological activity and chemical composition of P. cordoncillo worldwide.
Defining a New Classification System for the Surgical Management of Neuroendocrine Tumor Liver Metastases
Authors: Mahuron Kelly , Singh Gagandeep
Published: March 2023

Although rarely curative, hepatic cytoreduction of neuroendocrine tumor liver metastases (NETLM) is associated with improved symptom control and prolonged survival. Preoperative 68Ga DOTATATE and gadoxetic acid-enhanced liver MRI can improve characterization of hepatic disease extent to improve surgical clearance, and resection of the primary tumor is associated with improved survival regardless of whether the liver metastases are treated. As parenchymal-sparing surgical techniques and the lowering of the debulking threshold have expanded the numbers of eligible NETLM patients for hepatic cytoreduction, we propose a new classification system to help guide surgical management. A multimodal approach that includes surgery, liver-directed therapies, and systemic therapies has improved outcomes and increased longevity for patients with well-differentiated metastatic NET.
Repeated Measurements Are Necessary for Evaluating Accurate Diurnal Rhythm Using a Self-Intraocular Pressure Measurement Device
Authors: Shigemoto Yumi , Hasebe Yuka , Kitamura Kazuyoshi , Fukuda Yoshiko , Sakamoto Masako , Matsubara Mio , Minaguchi Shinya , Kashiwagi Kenji
Published: March 2023

Purpose: To investigate how many tests need to be performed to adequately assess intraocular pressure (IOP) diurnal change using a self-measuring rebound tonometer among glaucoma patients. Subjects and Methods: Adult patients with primary open-angle glaucoma were included. IOP was measured in the morning (6 AM to 9 AM), afternoon (12 PM to 3 PM), and at night (6 PM to 9 PM) for seven consecutive days. Twenty-four (7 males and 17 females, mean age 59.5 ± 11.0 years) patients who successfully measured IOP at least three times per day during the correct time periods for four days were subjected to analysis. Results: The IOP rhythm was significantly greater on the first day of measurement (6.6 ± 3.6 mmHg) than that averaged during subsequent days (4.4 ± 2.2 mmHg). The time of the highest and lowest IOP measurements on the first day of IOP measurement and during the entire measurement period coincided in 72.9% and 64.6% of cases, respectively. The concordance rate of the highest IOP time between the whole measurement period and each measurement day was less than 60%. Conclusion: The diurnal IOP rhythm measured by the patients themselves was not consistent, and multiple days of measurements may be necessary to correctly assess diurnal IOP rhythm.
A Meta-Analysis of Proton Pump Inhibitor Use and the Risk of Acute Kidney Injury: Geographical Differences and Associated Factors
Authors: Han Cheng Ta , Islam Md. Mohaimenul , Poly Tahmina Nasrin , Lu Yu-Chun , Lin Ming-Chin
Published: March 2023

Proton pump inhibitors (PPIs) are widely prescribed in medical practice for the treatment of several gastrointestinal disorders. Previous epidemiology studies have reported the association between PPI use and the risk of AKI, although the magnitude of the association between PPIs and the risk of acute kidney injury (AKI) remains uncertain. Therefore, we conducted a meta-analysis to determine the relationship between PPI therapy and the risk of AKI. We systematically searched for relevant articles published before January 2023 on PubMed, Scopus, and Web of Science. In addition, we conducted a manual search of the bibliographies of potential articles. Two independent reviewers examined the appropriateness of all studies for inclusion. We pooled studies that compared the risk of AKI with PPI against their control using a random effect model. The search criteria based on PRISMA guidelines yielded 568 articles. Twelve observational studies included 2,492,125 individuals. The pooled adjusted RR demonstrated a significant positive association between PPI therapy and the risk of AKI (adjusted RR 1.75, 95% CI: 1.40–2.19, p < 0.001), and it was consistent across subgroups. A visual presentation of the funnel plot and Egger’s regression test showed no evidence of publication bias. Our meta-analysis indicated that persons using PPIs exhibited an increased risk of AKI. North American individuals had a higher risk of AKI compared to Asian and European individuals. However, the pooled effect from observational studies cannot clarify whether the observed association is a causal effect or the result of some unmeasured confounding factors. Hence, the biological mechanisms underlying this association are still unclear and require further research.
Age and Comorbidity Burden of Patients Critically Ill with COVID-19 Affect Both Access to and Outcome of Ventilation Therapy in Intensive Care Units
Authors: de Hesselle Marie Louise , Borgmann Stefan , Rieg Siegbert , Vehreschild Jörg Janne , Rasch Sebastian , Koll Carolin E. M. , Hower Martin , Stecher Melanie , Ebert Daniel , Hanses Frank , Schumann Julia ,   on behalf of the LEOSS Study Group
Published: March 2023

During the COVID-19 pandemic, large numbers of elderly, multimorbid people required treatment in intensive care units. This study investigated how the inherent patient factors age and comorbidity burden affected the treatment strategy and the outcome achieved. Retrospective analysis of data from intensive care patients enrolled in the Lean European Open Survey on SARS-CoV2-Infected Patients (LEOSS) cohort found that a patient’s age and comorbidity burden in fact influenced their mortality rate and the use of ventilation therapy. Evidence showed that advanced age and multimorbidity were associated with the restrictive use of invasive ventilation therapies, particularly ECMO. Geriatric patients with a high comorbidity burden were clustered in the sub-cohort of non-ventilated ICU patients characterized by a high mortality rate. The risk of death generally increased with older age and accumulating comorbidity burden. Here, the more aggressive an applied procedure, the younger the age in which a majority of patients died. Clearly, geriatric, multimorbid COVID-19 patients benefit less from invasive ventilation therapies. This implies the need for a holistic approach to therapy decisions, taking into account the patient’s wishes.
Preventive Effect of Local Lidocaine Administration on the Formation of Traumatic Neuroma
Authors: Ji Feng , Zhang Yongyan , Cui Peng , Li Ying , Li Caixia , Du Dongping , Xu Hua
Published: March 2023

Background: Traumatic neuroma is a common sequela of peripheral nerve injury or amputation, which often leads to severe neuropathic pain. The present study investigated the effect of local lidocaine administration on preventing the formation of traumatic neuroma. Methods: Forty-eight male Sprague–Dawley rats were randomly assigned to two groups. The lidocaine group underwent sciatic nerve transection, followed by an injection of lidocaine (0.5%) around the proximal of a severed sciatic nerve under ultrasound-guidance 2–7 days after neurectomy. In the control group, rats received an injection of saline following neurectomy. The autotomy score, mechanical allodynia, thermal hyperalgesia, histological assessment, expression of neuroma, and pain-related markers were detected. Results: Lidocaine treatment reduced the autotomy score and attenuated mechanical allodynia and thermal hyperalgesia. The mRNA expression of α-SMA, NGF, TNF-α, and IL-1β all significantly decreased in the lidocaine group in comparison to those in the saline control group. The histological results showed nerve fibers, demyelination, and collagen hyperplasia in the proximal nerve stump in the saline control group, which were significantly inhibited in the lidocaine group. Conclusions: The present study demonstrated that local lidocaine administration could inhibit the formation of painful neuroma due to traumatic nerve injury.
AUV-Based Side-Scan Sonar Real-Time Method for Underwater-Target Detection
Authors: Tang Yulin , Wang Liming , Jin Shaohua , Zhao Jianhu , Huang Chao , Yu Yongcan
Published: March 2023

The limitations of underwater acoustic communications mean that the side-scan sonar data of an autonomous underwater vehicle (AUV) cannot be transmitted back and processed in real time, which means that targets cannot be detected in real time. To address the problem, this paper proposes an autonomous underwater vehicle-based side-scan sonar real-time detection method for underwater targets. First, the paper describes the system and operation of real-time underwater-target detection by the side-scan sonar mounted on the autonomous underwater vehicle. Next, it proposes a real-time processing method for side-scan sonar data, method for constructing a deep-learning-based underwater-target detection model, and real-time method for underwater-target detection based on navigation strip images, which, together, solve the three key technical problems of real-time data processing, deep-learning-based detection model construction, and real-time target detection based on the autonomous underwater vehicle. Finally, through sea-based experiments, the effectiveness of the proposed methods is evaluated, providing a new solution for the autonomous underwater vehicle-based side-scan sonar real-time detection of underwater targets.
Update on Risk Criteria for Crude Oil Tanker Fleet
Authors: Vidmar Peter , Perkovič Marko
Published: March 2023

In recent years, the maritime trade of crude oil has suffered notable perturbations caused by the unbalanced relationship between supply and demand. The COVID-19 pandemic caused a drop in oil consumption in 2019, followed by a reduction in production in 2020. The seaborne transport of oil accounts for approximately 50–60% of all crude oil in world production. The crude oil market is a crucial regulator of the global economy and instabilities in this market have noticeable effects on collective risks. The immediate risks that the society see are the changes in the cost of living, which are followed by political uncertainties. Less visible are the risks that these uncertainties have on shipping companies and the level of management stability they have to maintain in order to keep seagoing safe. This paper presents an update on the overall state of risk management for the crude oil tanker fleet, evidenced by EMSA and other international marine organisations. The previous paper, entitled Safety Assessment of Crude Oil Tankers, which applied the methodology of the Formal Safety Assessment (FSA), was published in 2018 and covered the historical data related to the fleet size, accident reports, amount of oil spilled on sea and the economic value of the crude oil transport business. The particular focus of this paper is on the evolution of the risk acceptance criteria over the years and the difference in the predictions from 2018 to the present day. The effects of the pandemic on crude oil shipping are discussed through the changes in the risks. Three of them are analysed: PLL (potential loss of lives), PLC (potential loss of containment) and PLP (potential loss of property). The representation of the risk applies the F-N curves among the risk acceptance criteria lines observed for different tanker sizes. Among the three risks, the paper exposes the vulnerability of the loss of containment risk, where the strong economic impact of the oil trade outweighs the environmental concerns. In relation to the PLC, the paper proposes the approach of relating the oil spill acceptability with the spill quantity and ship revenue instead of to the cost of cleaning or the cost of environment recovery.