bluuebunny/crossref_metadata_2025 huggingface dataset:
A Potential Therapy Using Antisense Oligonucleotides to Treat Autosomal Recessive Polycystic Kidney Disease
Authors: Li Huixia , Wang Chunli , Che Ruochen , Zheng Bixia , Zhou Wei , Huang Songming , Jia Zhanjun , Zhang Aihua , Zhao Fei , Ding Guixia
Published: February 2023

(1) Background: Autosomal recessive polycystic kidney disease (ARPKD) is a rare ciliopathy characterized by progressively enlarged kidneys with fusiform dilatation of the collecting ducts. Loss-of-function mutations in the PKHD1 gene, which encodes fibrocystin/polyductin, cause ARPKD; however, an efficient treatment method and drug for ARPKD have yet to be found. Antisense oligonucleotides (ASOs) are short special oligonucleotides which function to regulate gene expression and alter mRNA splicing. Several ASOs have been approved by the FDA for the treatment of genetic disorders, and many are progressing at present. We designed ASOs to verify whether ASOs mediate the correction of splicing further to treat ARPKD arising from splicing defects and explored them as a potential treatment option. (2) Methods: We screened 38 children with polycystic kidney disease for gene detection using whole-exome sequencing (WES) and targeted next-generation sequencing. Their clinical information was investigated and followed up. The PKHD1 variants were summarized and analyzed, and association analysis was carried out to analyze the relationship between genotype and phenotype. Various bioinformatics tools were used to predict pathogenicity. Hybrid minigene analysis was performed as part of the functional splicing analysis. Moreover, the de novo protein synthesis inhibitor cycloheximide was selected to verify the degraded pathway of abnormal pre-mRNAs. ASOs were designed to rescue aberrant splicing, and this was verified. (3) Results: Of the 11 patients with PKHD1 variants, all of them exhibited variable levels of complications of the liver and kidneys. We found that patients with truncating variants and variants in certain regions had a more severe phenotype. Two splicing variants of the PKHD1 genotypes were studied via the hybrid minigene assay: variants c.2141-3T>C and c.11174+5G>A. These cause aberrant splicing, and their strong pathogenicity was confirmed. We demonstrated that the abnormal pre-mRNAs produced from the variants escaped from the NMD pathway with the use of the de novo protein synthesis inhibitor cycloheximide. Moreover, we found that the splicing defects were rescued by using ASOs, which efficiently induced the exclusion of pseudoexons. (4) Conclusion: Patients with truncating variants and variants in certain regions had a more severe phenotype. ASOs are a potential drug for treating ARPKD patients harboring splicing mutations of the PKHD1 gene by correcting the splicing defects and increasing the expression of the normal PKHD1 gene.
Peripheral Refraction and Visual Function of Novel Perifocal Ophthalmic Lens for the Control of Myopia Progression
Authors: Silva-Leite Sara , Amorim-de-Sousa Ana , Queirós António , González-Méijome José Manuel , Fernandes Paulo
Published: February 2023

This study aimed to evaluate the peripheral defocus induced with a novel perifocal ophthalmic lens for myopia progression control and the potential impact on visual function. This experimental, non-dispensing crossover study evaluated 17 myopic young adults. The peripheral refraction was measured using an open-field autorefractor, at 2.50 m from the target point, in two eccentric points, 25° temporal, 25° nasal, and central vision. Visual contrast sensitivity (VCS) was measured at 3.00 m with a Vistech system VCTS 6500 in low light conditions. Light disturbance (LD) was assessed with a light distortion analyzer 2.00 m away from the device. Peripheral refraction, VCS, and LD were assessed with a monofocal lens and perifocal lens (with an add power of +2.50 D on the temporal side of the lens, and +2.00 D on the nasal side). The results showed that the perifocal lenses induced an average myopic defocus of −0.42 ± 0.38 D (p-value < 0.001) in the nasal retina, at 25° The changes induced by the lower add power in the nasal part of the lens did not induce statistically significant changes in the refraction of the temporal retina. The VCS and LD showed no significant differences between the monofocal and perifocal lenses.
Treatment Reality of Proximal Humeral Fractures in the Elderly—Trending Variants of Locking Plate Fixation in Germany
Authors: Rischen Robert , Köppe Jeanette , Stolberg-Stolberg Josef , Freistühler Moritz , Faldum Andreas , Raschke Michael , Katthagen J.
Published: February 2023

Background: The surgical treatment of proximal humeral fractures (PHFs) with locking plate fixation (LPF) in the elderly is associated with high complication rates, especially in osteoporotic bone. Variants of LPF such as additional cerclages, double plating, bone grafting and cement augmentation can be applied. The objective of the study was to describe the extent of their actual use and how this changed over time. Methods: Retrospective analysis of health claims data of the Federal Association of the Local Health Insurance Funds was performed, covering all patients aged 65 years and older, who had a coded diagnosis of PHF and were treated with LPF between 2010 and 2018. Differences between treatment variants were analyzed (explorative) via chi-squared or Kruskal–Wallis tests. Results: Of the 41,216 treated patients, 32,952 (80%) were treated with LPF only, 5572 (14%) received additional screws or plates, 1983 (5%) received additional augmentations and 709 (2%) received a combination of both. During the study period, relative changes were observed as follows: −35% for LPF only, +58% for LPF with additional fracture fixation and +25% for LPF with additional augmentation. Overall, the intra-hospital complication rate was 15% with differences between the treatment variants (LPF only 15%, LPF with additional fracture fixation 14%, LPF with additional augmentation 19%; p < 0.001), and a 30-day mortality of 2%. Conclusions: Within an overall decrease of LPF by approximately one-third, there is both an absolute and relative increase of treatment variants. Collectively, they account for 20% of all coded LPFs, which might indicate more personalized treatment pathways. The leading variant was additional fracture fixation using cerclages.
Data Resource Profile: The Multiple Sclerosis Documentation System 3D and AOK PLUS Linked Database (MSDS-AOK PLUS)
Authors: Ghiani Marco , Zhuleku Evi , Dillenseger Anja , Maywald Ulf , Fuchs Andreas , Wilke Thomas , Ziemssen Tjalf
Published: February 2023

Real-world evidence in multiple sclerosis (MS) is limited by the availability of data elements in individual real-world datasets. We introduce a novel, growing database which links administrative claims and medical records from an MS patient management system, allowing for the complete capture of patient profiles. Using the AOK PLUS sickness fund and the Multiple Sclerosis Documentation System MSDS3D from the Center of Clinical Neuroscience (ZKN) in Germany, a linked MS-specific database was developed (MSDS-AOK PLUS). Patients treated at ZKN and insured by AOK PLUS were recruited and asked for informed consent. For linkage, insurance IDs were mapped to registry IDs. After the deletion of insurance IDs, an anonymized dataset was provided to a university-affiliate, IPAM e.V., for further research applications. The dataset combines a complete record of patient diagnoses, treatment, healthcare resource use, and costs (AOK PLUS), with detailed clinical parameters including functional performance and patient-reported outcomes (MSDS3D). The dataset currently captures 500 patients; however, is actively expanding. To demonstrate its potential, we present a use case describing characteristics, treatment, resource use, and costs of a patient subsample. By linking administrative claims to clinical information in medical charts, the novel MSDS-AOK PLUS database can increase the quality and scope of real-world studies in MS.
Radiation Recall Pneumonitis: The Open Challenge in Differential Diagnosis of Pneumonia Induced by Oncological Treatments
Authors: Grassi Francesca , Granata Vincenza , Fusco Roberta , De Muzio Federica , Cutolo Carmen , Gabelloni Michela , Borgheresi Alessandra , Danti Ginevra , Picone Carmine , Giovagnoni Andrea , Miele Vittorio , Gandolfo Nicoletta , Barile Antonio , Nardone Valerio , Grassi Roberta
Published: February 2023

The treatment of primary and secondary lung neoplasms now sees the fundamental role of radiotherapy, associated with surgery and systemic therapies. The improvement in survival outcomes has also increased attention to the quality of life, treatment compliance and the management of side effects. The role of imaging is not only limited to recognizing the efficacy of treatment but also to identifying, as soon as possible, the uncommon effects, especially when more treatments, such as chemotherapy, immunotherapy and radiotherapy, are associated. Radiation recall pneumonitis is an uncommon treatment complication that should be correctly characterized, and it is essential to recognize the mechanisms of radiation recall pneumonitis pathogenesis and diagnostic features in order to promptly identify them and adopt the best therapeutic strategy, with the shortest possible withdrawal of the current oncological drug. In this setting, artificial intelligence could have a critical role, although a larger patient data set is required.
Quantitative High Density EEG Brain Connectivity Evaluation in Parkinson’s Disease: The Phase Locking Value (PLV)
Authors: di Biase Lazzaro , Ricci Lorenzo , Caminiti Maria Letizia , Pecoraro Pasquale Maria , Carbone Simona Paola , Di Lazzaro Vincenzo
Published: February 2023

Introduction: The present study explores brain connectivity in Parkinson’s disease (PD) and in age matched healthy controls (HC), using quantitative EEG analysis, at rest and during a motor tasks. We also evaluated the diagnostic performance of the phase locking value (PLV), a measure of functional connectivity, in differentiating PD patients from HCs. Methods: High-density, 64-channels, EEG data from 26 PD patients and 13 HC were analyzed. EEG signals were recorded at rest and during a motor task. Phase locking value (PLV), as a measure of functional connectivity, was evaluated for each group in a resting state and during a motor task for the following frequency bands: (i) delta: 2–4 Hz; (ii) theta: 5–7 Hz; (iii) alpha: 8–12 Hz; beta: 13–29 Hz; and gamma: 30–60 Hz. The diagnostic performance in PD vs. HC discrimination was evaluated. Results: Results showed no significant differences in PLV connectivity between the two groups during the resting state, but a higher PLV connectivity in the delta band during the motor task, in HC compared to PD. Comparing the resting state versus the motor task for each group, only HCs showed a higher PLV connectivity in the delta band during motor task. A ROC curve analysis for HC vs. PD discrimination, showed an area under the ROC curve (AUC) of 0.75, a sensitivity of 100%, and a negative predictive value (NPV) of 100%. Conclusions: The present study evaluated the brain connectivity through quantitative EEG analysis in Parkinson’s disease versus healthy controls, showing a higher PLV connectivity in the delta band during the motor task, in HC compared to PD. This neurophysiology biomarkers showed the potentiality to be explored in future studies as a potential screening biomarker for PD patients.
Polycystic Ovary Syndrome: Etiology, Current Management, and Future Therapeutics
Authors: Singh Samradhi , Pal Namrata , Shubham Swasti , Sarma Devojit Kumar , Verma Vinod , Marotta Francesco , Kumar Manoj
Published: February 2023

Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder, typically characterized by anovulation, infertility, obesity, insulin resistance, and polycystic ovaries. Lifestyle or diet, environmental pollutants, genetics, gut dysbiosis, neuroendocrine alterations, and obesity are among the risk factors that predispose females to PCOS. These factors might contribute to upsurging metabolic syndrome by causing hyperinsulinemia, oxidative stress, hyperandrogenism, impaired folliculogenesis, and irregular menstrual cycles. Dysbiosis of gut microbiota may play a pathogenic role in the development of PCOS. The restoration of gut microbiota by probiotics, prebiotics, or a fecal microbiota transplant (FMT) might serve as an innovative, efficient, and noninvasive way to prevent and mitigate PCOS. This review deliberates on the variety of risk factors potentially involved in the etiology, prevalence, and modulation of PCOS, in addition to plausible therapeutic interventions, including miRNA therapy and the eubiosis of gut microbiota, that may help treat and manage PCOS.
Understanding the Role of Ureteral Access Sheath in Preventing Post-Operative Infectious Complications in Stone Patients Treated with Ureteroscopy and Ho:YAG Laser Lithotripsy: Results from a Tertiary Care Referral Center
Authors: Villa Luca , Dioni Pietro , Candela Luigi , Ventimiglia Eugenio , De Angelis Mario , Corsini Christian , Robesti Daniele , Fantin Margherita , D’Arma Alessia , Proietti Silvia , Giusti Guido , Kartalas Goumas Ioannis , Briganti Alberto , Montorsi Francesco , Salonia Andrea
Published: February 2023

Introduction and objectives: The use of ureteral access sheaths (UAS) limits the irrigation-induced increase in intrarenal pressure during ureteroscopy (URS). We investigated the relationship between UAS and rates of postoperative infectious complications in stone patients treated with URS. Materials and methods: Data from 369 stone patients treated with URS from September 2016 to December 2021 at a single institution were analyzed. UAS (10/12 Fr) placement was attempted in case of intrarenal surgery. The chi-square test was used to assess the relationship between the use of UAS and fever, sepsis, and septic shock. Univariable and multivariable logistic regression analyses tested the association of patients’ characteristics and operative data and the rate of postoperative infectious complications. Results: Full data collection of 451 URS procedures was available. Overall, UAS was used in 220 (48.8%) procedures. As for postoperative infectious sequalae, we recorded fever (n = 52; 11.5%), sepsis (n = 10; 2.2%), and septic shock (n = 6; 1.3%). Of those, UAS was not used in 29 (55.8%), 7 (70%), and 5 (83.3%) cases, respectively (all p > 0.05). At multivariable logistic regression analysis, performing URS without UAS was not associated with the risk of having fever and sepsis, but it increased the risk of septic shock (OR = 14.6; 95% CI = 1.08–197.1). Moreover, age-adjusted CCI score (for fever-OR = 1.23; 95% CI = 1.07–1.42, sepsis-OR = 1.47; 95% CI = 1.09–1.99, and septic shock-OR = 1.61; 95% CI = 1.08–2.42, respectively), history of fever secondary to stones (for fever-OR = 2.23; 95% CI = 1.02–4.90) and preoperative positive urine culture (for sepsis-OR = 4.87; 95% CI = 1.12–21.25) did emerge as further associated risk factors. Conclusions: The use of UAS emerged to prevent the onset of septic shock in patients treated with URS, with no clear benefit in terms of fever and sepsis. Further studies may help clarify whether the reduction in fluid reabsorption load mediated by UAS is protective against life-threatening conditions in case of infectious complications. The patients’ baseline characteristics remain the main predictors of infectious sequelae in a clinical setting.
Effects of Individualised High Positive End-Expiratory Pressure and Crystalloid Administration on Postoperative Pulmonary Function in Patients Undergoing Robotic-Assisted Radical Prostatectomy: A Prospective Randomised Single-Blinded Pilot Study
Authors: Blecha Sebastian , Hager Anna , Gross Verena , Seyfried Timo , Zeman Florian , Lubnow Matthias , Burger Maximilian , Pawlik Michael T.
Published: February 2023

Objectives: Robotic-assisted laparoscopic prostatectomy (RALP) is typically conducted in steep Trendelenburg position (STP). The aim of the study was to evaluate whether crystalloid administration and individual management of positive end-expiratory pressure (PEEP) improve peri- and post-operative pulmonary function in patients undergoing RALP. Design: Prospective randomised single-centre single-blinded explorative study. Setting: Patients were either allocated to a standard PEEP (5 cmH2O) group or an individualised high PEEP group. Furthermore, each group was divided into a liberal and a restrictive crystalloid group (8 vs. 4 mL/kg/h predicted body weight). Individualised PEEP levels were determined by means of preoperative recruitment manoeuvre and PEEP titration in STP. Participants: Informed consent was obtained from 98 patients scheduled for elective RALP. Interventions: The following intraoperative parameters were analysed in each of the four study groups: ventilation setting (peak inspiratory pressure [PIP], plateau pressure, driving pressure [Pdriv], lung compliance [LC] and mechanical power [MP]) and postoperative pulmonary function (bed-side spirometry). The spirometric parameters Tiffeneau index (FEV1/FVC ratio) and mean forced expiratory flow (FEF25–75) were measured pre- and post-operatively. Data are shown as mean ± standard deviation (SD), and groups were compared with ANOVA. A p-value of <0.05 was considered significant. Results: The two individualised high PEEP groups (mean PEEP 15.5 [±1.71 cmH2O]) showed intraoperative significantly higher PIP, plateau pressure and MP levels but significantly decreased Pdriv and increased LC. On the first and second postoperative day, patients with individualised high PEEP levels had a significantly higher mean Tiffeneau index and FEF25–75. Perioperative oxygenation and ventilation and postoperative spirometric parameters were not influenced by restrictive or liberal crystalloid infusion in either of the two respective PEEP groups. Conclusions: Individualised high PEEP levels (≥14 cmH2O) during RALP improved intraoperative blood oxygenation and resulted in more lung-protective ventilation. Furthermore, postoperative pulmonary function was improved for up to 48 h after surgery in the sum of the two individualised high PEEP groups. Restrictive crystalloid infusion during RALP seemed to have no effect on peri- and post-operative oxygenation and pulmonary function.
The Profile and All-Cause In-Hospital Mortality Dynamics of St-Segment Elevation Myocardial Infarction Patients during the Two Years of the COVID-19 Pandemic
Authors: Popa-Fotea Nicoleta-Monica , Grigore Iulia-Adelina , Calmac Lucian , Mihai Cosmin , Bataila Vlad , Ploscaru Vlad , Dragoescu Bogdan , Moldovan Horatiu , Busnatu Stefan-Sebastian , Panaitescu Eugenia , Iliuță Luminita , Scafa-Udriște Alexandru
Published: February 2023

During the coronavirus pandemic 2019 (COVID-19), some studies showed differences in the profile of subjects presenting with acute coronary syndromes as well as in overall mortality due to the delay of presentation and other complications. The purpose of this study was to compare the profile and outcomes, with emphasis on all-cause in-hospital mortality, of ST-elevation myocardial infarction (STEMI) subjects presenting to the emergency department during the pandemic period compared with a control group from the previous year, 2019. The study enrolled 2011 STEMI cases, which were divided into two groups—pre-pandemic (2019–2020) and pandemic period (2020–2022). Hospital admissions for a STEMI diagnosis sharply decreased during the COVID-19 period by 30.26% during the first year and 25.4% in the second year. This trend was paralleled by a significant increase in all-cause in-hospital mortality: 11.5% in the pandemic period versus 8.1% in the previous year. There was a significant association between SARS-CoV-2 positivity and all-cause in-hospital mortality, but no correlation was found between COVID-19 diagnosis and the type of revascularization. However, the profile of subjects presenting with STEMI did not change over time during the pandemic; their demographic and comorbid characteristics remained similar.
Clinical Profiles and Prognoses of Adult Patients with Full-Frequency Sudden Sensorineural Hearing Loss in Combination Therapy
Authors: Zhu Yuanping , He Sihai , Liao Kang , Li Meihua , Zhao Zhibin , Jiang Hongyan
Published: February 2023

We aimed to characterize the clinical profiles and short-term outcomes of adult patients with full-frequency idiopathic sudden sensorineural hearing loss (ISSNHL) treated uniformly with combination therapy, and to determine the prognostic predictors for the combination therapy. A total of 131 eligible cases hospitalized in our department from January 2018 to June 2021 were retrospectively reviewed. All enrolled cases received a standardized combination therapy employing intravenous methylprednisolone, batroxobin, and Ginkgo biloba extract during the 12 days of hospitalization. The clinical and audiometric profiles were compared between recovered patients and their unrecovered counterparts. The overall recovery rate was 57.3% in the study. Accompanying vertigo (odds ratio = 0.360, p = 0.006) and body mass index (BMI, odds ratio = 1.158, p = 0.016) were two independent predictors of hearing outcomes of the therapy. The male gender and cigarette-smoking history were marginally associated with good hearing prognosis (p = 0.051 and 0.070, respectively). Patients with BMI ≥ 22.4 kg/m2 had a better chance of hearing recovery (p = 0.02). Conclusions: Accompanying vertigo and low BMI (<22.4 kg/m2) were independently associated with poor prognosis for full-frequency ISSNHL in combination therapy. Male gender and cigarette-smoking history might be considered positive effects on hearing prognosis.
Post-Surgical Imaging Assessment in Rectal Cancer: Normal Findings and Complications
Authors: De Muzio Federica , Fusco Roberta , Cutolo Carmen , Giacobbe Giuliana , Bruno Federico , Palumbo Pierpaolo , Danti Ginevra , Grazzini Giulia , Flammia Federica , Borgheresi Alessandra , Agostini Andrea , Grassi Francesca , Giovagnoni Andrea , Miele Vittorio , Barile Antonio , Granata Vincenza
Published: February 2023

Rectal cancer (RC) is one of the deadliest malignancies worldwide. Surgery is the most common treatment for RC, performed in 63.2% of patients. The type of surgical approach chosen aims to achieve maximum residual function with the lowest risk of recurrence. The selection is made by a multidisciplinary team that assesses the characteristics of the patient and the tumor. Total mesorectal excision (TME), including both low anterior resection (LAR) and abdominoperineal resection (APR), is still the standard of care for RC. Radical surgery is burdened by a 31% rate of major complications (Clavien–Dindo grade 3–4), such as anastomotic leaks and a risk of a permanent stoma. In recent years, less-invasive techniques, such as local excision, have been tested. These additional procedures could mitigate the morbidity of rectal resection, while providing acceptable oncologic results. The “watch and wait” approach is not a globally accepted model of care but encouraging results on selected groups of patients make it a promising strategy. In this plethora of treatments, the radiologist is called upon to distinguish a physiological from a pathological postoperative finding. The aim of this narrative review is to identify the main post-surgical complications and the most effective imaging techniques.
Alcohol Consumption, Hangovers, and Smoking among Buenos Aires University Students during the COVID-19 Pandemic
Authors: Karadayian Analia , Merlo Agnese , Czerniczyniec Analia , Lores-Arnaiz Silvia , Hendriksen Pauline , Kiani Pantea , Bruce Gillian , Verster Joris
Published: February 2023

In Argentina, the 2019 coronavirus disease (COVID-19) pandemic led to serious changes to social interaction, health, economy, and education. Argentina experienced two extensive lockdown periods. University education remained virtual for almost two academic years. The purpose of the present work was to analyze the impact of the COVID-19 lockdowns in Argentina on alcohol consumption, hangover severity and smoking among university students in Buenos Aires. A retrospective online survey was conducted in 2021 among students of the University of Buenos Aires. Participants aged 18–35 years old were asked about the average number of alcoholic drinks and number of drinking days per week, binge drinking occasions, drunkenness, next day hangover severity, number of hangovers per month, and smoking behavior. The results showed that the first and second COVID-19 lockdowns were associated with significant reductions in both weekly alcohol consumption, and hangover severity and subjective intoxication on their heaviest drinking occasions. Males consumed significantly more alcohol than females, and older students (25–35 years old) consumed more alcohol than younger students (18–24 years old). In addition, younger students reduced the number of cigarettes smoked per day during the two lockdown periods while older students exhibited significantly more smoking days per week. In conclusion, the present work in Argentinian students revealed a significant reduction in weekly alcohol consumption, and subjective intoxication and hangover severity on their heaviest drinking occasions during the pandemic lockdown periods.
Antibacterial Activity and Cell Viability of Biomimetic Magnesian Calcite Coatings on Biodegradable Mg
Authors: Popa Monica , Anastasescu Mihai , Stefan Laura M. , Prelipcean Ana-Maria , Calderon Moreno Jose
Published: February 2023

Mg is a material of choice for biodegradable implants. The main challenge for using Mg in temporary implants is to provide protective surfaces that mitigate its rapid degradation in biological fluids and also confer sufficient cytocompatibility and bacterial resistance to Mg-coated surfaces. Even though carbonate mineralization is the most important source of biominerals, such as the skeletons and shells of many marine organisms, there has been little success in the controlled growth of carbonate layers by synthetic processes. We present here the formation mechanism, antibacterial activity, and cell viability of magnesian calcite biomimetic coatings grown on biodegradable Mg via a green, one-step route. Cell compatibility assessment showed cell viability higher than 80% after 72 h using fibroblast cells (NCTC, clone L929) and higher than 60% after 72 h using human osteoblast-like cells (SaOS-2); the cells displayed a normal appearance and a density similar to the control sample. Antimicrobial potential evaluation against both Gram-positive (Staphylococcus aureus (ATCC 25923)) and Gram-negative (Pseudomonas aeruginosa (ATCC 27853)) strains demonstrated that the coated samples significantly inhibited bacterial adhesion and biofilm formation compared to the untreated control. Calcite coatings grown on biodegradable Mg by a single coating process showed the necessary properties of cell compatibility and bacterial resistance for application in surface-modified Mg biomaterials for temporary implants.
Functional Approaches in Promoting Vascularization and Angiogenesis in Bone Critical-Sized Defects via Delivery of Cells, Growth Factors, Drugs, and Particles
Authors: Shineh Ghazal , Patel Kishan , Mobaraki Mohammadmahdi , Tayebi Lobat
Published: February 2023

Critical-sized bone defects, or CSDs, are defined as bone defects that cannot be regenerated by themselves and require surgical intervention via employing specific biomaterials and a certain regenerative strategy. Although a variety of approaches can be used to treat CSDs, poor angiogenesis and vascularization remain an obstacle in these methods. The complex biological healing of bone defects depends directly on the function of blood flow to provide sufficient oxygen and nutrients and the removal of waste products from the defect site. The absence of vascularization can lead to non-union and delayed-union defect development. To overcome this challenge, angiogenic agents can be delivered to the site of injury to stimulate vessel formation. This review begins by introducing the treatment methods for CSDs. The importance of vascularization in CSDs is subsequently highlighted. Delivering angiogenesis agents, including relevant growth factors, cells, drugs, particles, cell secretion substances, their combination, and co-delivery to CSDs are fully explored. Moreover, the effects of such agents on new bone formation, followed by vessel formation in defect areas, are evaluated.
Effects of Resistance Training in Individuals with Lower Limb Amputation: A Systematic Review
Authors: Rosario Miguel L. V. V. , Costa Pablo B. , da Silveira Anderson L. B. , Florentino Kairos R. C. , Casimiro-Lopes Gustavo , Pimenta Ricardo A. , Dias Ingrid , Bentes Claudio Melibeu
Published: February 2023

Individuals with lower-limb amputations may have a significant strength deficit. This deficit may be related to the stump length and can lead to changes in gait, reduced energy efficiency, walking resistance, altered joint load, and increased risk of osteoarthritis and chronic low back pain. This systematic review used the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) guidelines to examine the effects of resistance training in lower limb amputees. Interventions with resistance training and other training methods were sufficient to achieve muscle strength gain in muscles of the lower limbs, improved balance, and improvements in gait pattern and speed when walking. However, it was impossible to determine from the results whether resistance training was mainly responsible for these benefits or even whether the positive effects presented would be observed with only this training method. When combined with other exercises, interventions with resistance training made possible gains for this population. Accordingly, it is noteworthy that the main finding of this systematic review is that the effects may be different according to the level of amputation, with mainly transtibial and transfemoral amputations studied.
Forensic Gender Determination by Using Mandibular Morphometric Indices an Iranian Population: A Panoramic Radiographic Cross-Sectional Study
Authors: Esfehani Mahsa , Ghasemi Melika , Katiraee Amirhassan , Tofangchiha Maryam , Alizadeh Ahad , Taghavi-Damghani Farnaz , Testarelli Luca , Reda Rodolfo
Published: February 2023

Gender determination is the first step in forensic identification, followed by age and height determination, which are both affected by gender. This study assessed the accuracy of gender estimation using mandibular morphometric indices on panoramic radiographs of an Iranian population. This retrospective study evaluated 290 panoramic radiographs (145 males and 145 females). The maximum and minimum ramus width, coronoid height, condylar height, antegonial angle, antegonial depth, gonial angle, and the superior border of mental foramen were bilaterally measured as well as bicondylar and bigonial breadths using Scanora Lite. Correlation of parameters with gender was analyzed by univariate, multiple, and best models. All indices except for gonial angle were significantly different between males and females and can be used for gender determination according to univariate model. Condylar height, coronoid height, and superior border of mental foramen and ramus were still significantly greater in males than in females after controlling for the effect of confounders (p < 0.05). Based on the best model, a formula including five indices of bicondylar breadth, condylar height, coronoid height, minimum ramus width, and superior border of mental foramen was used for gender determination. Values higher than 56% indicate male gender, while lower values indicate female gender, with 81.38% specificity for correct detection of females and 88.97% sensitivity for correct detection of males. Despite the satisfactory results, future research should focus on larger populations to verify the accuracy of the present findings.
Self-Parameterized Chaotic Map for Low-Cost Robust Chaos
Authors: Paul Partha Sarathi , Dhungel Anurag , Sadia Maisha , Hossain Md Razuan , Hasan Md Sakib
Published: February 2023

This paper presents a general method, called “self-parameterization”, for designing one-dimensional (1-D) chaotic maps that provide wider chaotic regions compared to existing 1-D maps. A wide chaotic region is a desirable property, as it helps to provide robust performance by enlarging the design space in many hardware-security applications, including reconfigurable logic and encryption. The proposed self-parameterization scheme uses only one existing chaotic map, referred to as the seed map, and a simple transformation block. The effective control parameter of the seed map is treated as an intermediate variable derived from the input and control parameter of the self-parameterized map, under some constraints, to achieve the desired functionality. The widening of the chaotic region after adding self-parameterization is first demonstrated on three ideal map functions: Logistic; Tent; and Sine. A digitized version of the scheme was developed and realized in a field-programmable gate array (FPGA) implementation. An analog version of the proposed scheme was developed with very low transistor-count analog topologies for hardware-constrained integrated circuit (IC) implementation. The chaotic performance of both digital and analog implementations was evaluated with bifurcation plots and four established chaotic entropy metrics: the Lyapunov Exponent; the Correlation Coefficient; the Correlation Dimension; and Approximate Entropy. An application of the proposed scheme was demonstrated in a random number generator design, and the statistical randomness of the generated sequence was verified with the NIST test.
Air-Backed Aluminum Shells Subjected to Underwater Penetration: Torpedo Interception Simulations
Authors: Peng Chucai , Ding Chufan , Chen Bin , Sun Xichen , Li Weibing , Qin Taotao , Feng Jun
Published: February 2023

Underwater torpedoes have become a serious threat to ocean liners and warships, and the interception against attacking torpedoes is always the hotspot in marine engineering. To simulate the underwater torpedo interception by a high velocity projectile, this work numerically deals with the process of projectile water entry and sequent penetration into underwater aluminum shells, whereby conical and ogival nose projectiles are comparatively studied. With the arbitrary Lagrange–Euler (ALE) algorithm adopted to describe fluid medium, the projectile water entry model is developed and validated against the test data. Similarly, the penetration model validation is made by modeling a tungsten ball perforation on an aluminum plate. Covered by water fluid, the air-backed aluminum shell is utilized to simulate an underwater torpedo subjected to projectile impact. The numerical predictions of underwater penetration reveal that ogival nose projectiles have a superior performance in underwater motion and perforation while conical nose counterparts deteriorate the shell targets more severely. For 20 cm, 40 cm and 60 cm underwater depth scenarios, a numerical prediction suggests that the energy consumed by water is proportional to the water depth, meanwhile aluminum shell perforation absorbs almost the identical projectile kinetic energy. Such findings may shed some light on the nose shape optimization design of high velocity projectile intercepting underwater torpedoes.
Optimal SOC Control and Rule-Based Energy Management Strategy for Fuel-Cell-Based Hybrid Vessel including Batteries and Supercapacitors
Authors: Ma Zeyu , Chen Hao , Han Jingang , Chen Yizheng , Kuang Jiongchen , Charpentier Jean-Frédéric , Aϊt-Ahmed Nadia , Benbouzid Mohamed
Published: February 2023

Around the world, the development of electric vehicles is underway, including in maritime transportation. However, the development of clean energy vessels still has a long way to go. Fuel cells (FCs) are a relevant choice among the many clean energy sources to power clean energy vessels. However, due to the complex and drastic change in the shipload power, FCs need to be equipped with dynamic fast-response energy storage equipment to make up for it. For multiple energy storage devices connected in parallel, the state of charge (SOC) is not balanced, which affects their service life and the stability of the vessel microgrid, as well as slowing the speed and lowering the accuracy of SOC equalization. This paper proposes a distributed variable sag slope control strategy for vessels to improve SOC equalization, with a FC as the energy source and a battery and supercapacitor as the energy storage system (ESS). For the output power distribution problem of energy storage equipment caused by shipload power variation, a power distribution strategy with a variable filter time constant is used to improve the reasonableness of the output power distribution of energy-based lithium batteries and power-based supercapacitors. Meanwhile, this paper considers the power generation equipment’s service life and energy cost as the optimization objectives, considering the discharge depth of the energy storage equipment. Finally, a method based on the combination of the lithium battery SOC rule (the rule formulated according to the state of charge and load power change in energy storage equipment) and particle swarm optimization algorithm is proposed to solve this problem. The simulation results show that the proposed strategy improves the equalization speed and accuracy of the SOC of energy storage devices, fully realizes the characteristics of different energy storage devices, and reduces the life loss of energy storage devices.
Container Ship Fleet Route Evaluation and Similarity Measurement between Two Shipping Line Ports
Authors: Šakan Davor , Žuškin Srđan , Rudan Igor , Brčić David
Published: February 2023

The characterization of ship routes and route similarity measurement based on Automatic Identification System (AIS) data are topics of various scientific interests. Common route research approaches use available AIS identifiers of ship types. However, assessing route and similarity profiles for individual fleets requires collecting data from secondary sources, dedicated software libraries or the creation of specific methods. Using an open-source approach, public AIS and ship data, we evaluate route characteristics for the container ships of a single fleet in a six-month period, calling on two selected ports of the shipping line on the USA East Coast. We evaluate the routes in terms of length, duration and speed, whereas for the similarity measurement we employ the discrete Fréchet distance (DFD). The voyage length, duration and average speed distributions were observed to be moderately positive (0.77), negative (−0.62), and highly positively skewed based on the adjusted Fisher–Pearson coefficient of skewness (1.23). The most similar voyages were from the same ships, with the lowest discrete Fréchet distance similarity value (0.9 NM), whereas 2 different ships had the most dissimilar voyages, with the highest DFD value (14.1 NM). The proposed methodology enables assessment of similarities between individual ships, or between fleets.
The Application and Research of New Digital Technology in Marine Aquaculture
Authors: Zhang Hanwen , Gui Fukun
Published: February 2023

Marine aquaculture has become an important strategy to enable the ecological and sustainable development of fishery due to the decreasing natural fishery resources. To solve farming pain points, improve farming efficiency and modernize fisheries, new digital technologies, such as the Internet of Things, big data, cloud computing, artificial intelligence and blockchain, are increasingly being widely applied in aquaculture. This paper introduces the interrelationship of new digital technologies and the framework of their application in marine aquaculture. The results of the application of each new digital technology in marine aquaculture are highlighted, and the advantages or problems of each new digital technology in marine aquaculture are pointed out. Further, the application of new digital technologies in deep-sea aquaculture facilities is enumerated. Finally, the main problems faced by new digital technologies in the process of marine aquaculture production and the future development trend are sorted out and summarized to provide scientific reference for promoting the wide application of new digital technology in marine aquaculture.
Ship Trajectory Clustering Based on Trajectory Resampling and Enhanced BIRCH Algorithm
Authors: Yan Zhaojin , Yang Guanghao , He Rong , Yang Hui , Ci Hui , Wang Ran
Published: February 2023

Automatic identification systems (AIS) provides massive ship trajectory data for maritime traffic management, route planning, and other research. In order to explore the valuable ship traffic characteristics contained implicitly in massive AIS data, a ship trajectory clustering method based on ship trajectory resampling and enhanced BIRCH (Balanced Iterative Reducing and Clustering using Hierarchies) algorithm is proposed. The method has been tested using 764,393 AIS trajectory points of 13,845 ships in the waters of the Taiwan Strait of China, and 832 ship trajectories have been generated and clustered to obtain 172 classes of ship trajectory line clusters among 40 port pairs. The experimental results show that the proposed method has exhibited a good clustering effect on ship trajectories. Compared with the existing ship trajectory clustering methods, the proposed method can more efficiently detect and identify differences between trajectories with largely similar spatial distribution characteristics, so as to obtain legitimate clustering results. In addition, this study has constructed the main ship navigation routes between ports based on the extracted ship trajectory line clusters, and the constructed main routes are directional, refined, and rich in content compared with the existing ship routes. This research provides theoretical and technical support for ship route planning and maritime traffic management.
Study on the Low-Frequency and Broadband Sound Absorption Performance of an Underwater Anechoic Layer with Novel Design
Authors: Hu Jinshun , Lin Yongshui , Zhou Zhiwei , Cao Xiaofei , Chi Qingjia , Wu Weiguo
Published: February 2023

To further improve the low-frequency broadband sound absorption capability of the underwater anechoic layer (UAL) on the surface of marine equipment, a novel sound absorption structure with cavities (NSSC) is designed by adding resonators and honeycombs to the traditional sound absorption structure with cavities (SSC). Based on the principle of shear dissipation, the original intention of the design is to allow more parts of the viscoelastic material to participate the dissipation of acoustic energy. The approximate multilayer sound absorption theoretical model based on the modified transfer matrix method is used to verify the accuracy of finite element calculations. In the frequency range of 1100 Hz–10,000 Hz, the sound absorption coefficient (α) of NSSC can reach 0.8. The effects of the presence and size of cylindrical oscillators and honeycomb structures on sound absorption are discussed in detail. The results show that expanding the effective sound absorption range of the damping area of the structure is the key to improve the wideband sound absorption effect. This design concept could guide the structural design of the UAL.
Statistical Analysis of Accidents and Review of Safety Level of Passenger Ships
Authors: Eliopoulou Eleftheria , Alissafaki Aimilia , Papanikolaou Apostolos
Published: February 2023

This paper deals with a statistical analysis of maritime accidents pertaining to passenger ships in worldwide operation and, ultimately, with the assessment of the current safety level of the particular ship type, assuming that the safety level may be defined as a societally acceptable level of risk. The basic risk contributors, namely the frequency of main accidents’ occurrence and related consequences, are herein quantified and assessed. The presented analysis, covering the last two decades (2000–2021), shows that there has been a considerable reduction in the frequency of serious accidents’ occurrence in the last decade, whereas associated consequences do not exhibit the same trend since the frequency of ship total losses and of fatalities has increased. However, fatality rates are, to a great extent, affected by accidents of ships not compliant with safety regulations. This study also confirmed that grounding and contact accidents dominate the statistics of passenger ships of all subtypes, whereas collision accidents only account for about 30% of the events potentially leading to flooding. This calls for an urgent reconsideration of present SOLAS damage stability regulations, in which the bottom and side collision events need to be reconsidered with priority as the prime contributors to the flooding risk of passenger ships.
Monitoring the Kotychi Lagoon in Western Peloponnese, Greece, Using Remote Sensing Techniques and Environmental Assessment
Authors: Apostolopoulos Dionysios N. , Giannikopoulos Dionysios , Ramfos Alexis , Faulwetter Sara , Panagiotaras Dionisios , Nikolakopoulos Konstantinos G. , Avramidis Pavlos
Published: February 2023

The Kotychi Lagoon (western Peloponnese, Greece), together with other coastal wetlands such as Pappas, Prokopos and Lamia, is part of the wider area of the Strofylia Wetlands National Park and Protected Areas of Western Peloponnese, which has been protected by the Ramsar convention since 1971 and classified as a Natura 2000 area due to its high diversity and ecological value. This study aimed to estimate the water surface evolution of the Kotychi Lagoon using optical remote sensing techniques to record parameters and indices related to the geometrical and landscape orientation features for the 1945–2016 period and to evaluate the ecological and environmental status of the lagoon through in-situ measurement and sample analysis. High-resolution aerial photos at a scale of 1:30,000, orthomosaics, and satellite images were used and linear regression rates (LRR) were calculated to determine the changes. Seasonal sampling of water, sediment, and macrofaunal organisms in the lagoon was performed to monitor environmental and ecological parameters. The results showed that the lagoon’s water surface shrank during the 1945–2016 period, showing different rates in four segments. The shrinkage prevailed in the northeastern and southern parts of the lagoon, with rates of 17.75 and 6.46 m/year, respectively. The ecological status was determined to be poor, although nutrient input seemed to have been moderate over the study period. We conclude that applying remote sensing techniques using a combination of high-resolution images constitutes an effective and accurate method for the spatiotemporal mapping of wetlands. The ecological problems of the Kotychi Lagoon were highlighted and confirmed through complementary in-situ analyses. These problems need to be addressed by the respective managerial bodies in order to restore the lagoon to good ecological status.