bluuebunny/crossref_metadata_2025 huggingface dataset:
A Metastatic Breast Tumor of an Appendiceal Signet Ring Cell Carcinoma
Authors: Okumura Kenta , Oura Shoji
Published: April 2023

A 54-year-old woman with pseudomyxoma peritonei from an appendiceal signet ring cell carcinoma was referred to our hospital. Right massive effusion with cytology-proven malignant cells was controlled with thoracentesis. Pathological study after intraperitoneal (IP) chemotherapy, hyperthermic IP chemotherapy, and cytoreductive surgery showed no malignant cells in the abdomen except for the appendix and greater omentum. Although the patient noticed a right breast mass, mammography (MMG) showed no abnormality. Ultrasonography showed right breast masses consisting of hypo- and hyper-echoic areas without clear tumor margins. Magnetic resonance imaging (MRI) with contrast medium showed multiple lesions showing persistent enhancement pattern. Pathological study of the vacuum-assisted biopsy specimen showed signet ring cells growing in diffuse, trabecular, and linear fashions, leading to the diagnosis of metastatic breast tumors from the appendiceal signet ring cell carcinoma. Positron emission tomography (PET)/computed tomography (CT) showed no fluorodeoxyglucose uptake in the breasts. The patient was treated with simple mastectomy for local control. Pathological study of the resected breast showed predominant localization of the metastatic breast tumors deep in the mammary gland and lymphovascular invasion. Metastatic breast tumors from appendiceal signet ring cell carcinomas are extremely rare and can sometimes be difficult to detect with MMG and PET/CT. General surgeon should note that appendiceal signet ring cell carcinomas can metastasize to the breast, presumably through lymphatic permeation from malignant pleural effusion, without abnormal MMG and PET/CT findings.
Type I Thyroplasty Is Safe in Patients Undergoing Antithrombotic Therapy
Authors: Sawatsubashi Motohiro , Umezaki Toshiro , Kikuchi Yoshikazu , Adachi Kazuo , Nakagawa Takashi
Published: April 2023

Introduction: Type I thyroplasty is one of the most useful surgeries for unilateral vocal fold paralysis. The study objective was to determine whether type I thyroplasty is safe and perioperative antithrombotic management is acceptable in patients undergoing antithrombotic therapy. Methods: This is a single-hospital retrospective cohort study. The records of 204 patients who underwent type I thyroplasty at a Japanese university hospital, between 2008 and July 2018 were reviewed. We compared the prothrombin time international normalized ratio, prothrombin time, operative time, intraoperative blood loss, and intra- and postoperative complications between patients who did and did not receive antithrombotic therapy. Results: Of 204 patients, 51 (25%) received antithrombotic therapy (antithrombotic group). The remaining 153 patients were assigned to the control group. There were no significant differences in operative time, intraoperative blood loss, or intraoperative complications between the two groups. Sixteen (31%) patients in the antithrombotic group had a hemorrhage or hematoma in the vocal fold mucosa postoperatively, no patient had airway obstruction necessitating tracheostomy, and all patients recovered with follow-up observation only. There were no cases of intraoperative or postoperative complications, such as ischemic heart disease, ischemic stroke, or deep vein thrombosis. Conclusion: Type I thyroplasty with careful pre- and postoperative management is safe in patients undergoing antithrombotic therapy.
High Incidence of Signs of Neuropathy and Self-Reported Substance Use Disorder for Nitrous Oxide in Patients Intoxicated with Nitrous Oxide
Authors: Nugteren – Van Lonkhuyzen Johanna J. , van der Ben Lot , van den Hengel- Koot Irma S. , de Lange Dylan W. , van Riel Antoinette J.H.P. , Hondebrink Laura
Published: April 2023

Introduction: The number of patients with excessive nitrous oxide (N2O) use and neurological disorders has been rising, indicating an addictive potential of N2O. We studied the incidence of self-reported substance use disorder (SUD)-related symptoms, signs of neuropathy, and the patterns of use in N2O-intoxicated patients. Methods: The Dutch Poisons Information Center (DPIC) provides information by telephone on the management of intoxications to healthcare professionals. Retrospective data on signs of neuropathy and patterns of use were collected for all N2O intoxications reported to the DPIC in 2021 and 2022. Frequent and heavy use were self-reported as “often/frequent/weekly use” and as “use of tanks or >50 balloons/session,” respectively. From this cohort, we included patients with excessive N2O use or signs of neuropathy in a prospective observational cohort study. Online surveys were sent 1 week, 1 month, and 3 months after DPIC consultation. The survey included the drug use disorder questionnaire (validated to measure self-reported substance abuse [SA] and substance dependence [SD] based on Diagnostic and Statistical Manual of Mental Disorders [DSM]-IV-TR criteria) and questions on patterns of use and signs of neuropathy. DSM-IV-TR criteria were translated to DSM-V criteria to score for mild, moderate, or severe SUD, with 2–3, 4–5, or ≥6 symptoms, respectively. Results: We included 101 N2O-intoxicated patients in the retrospective study. Of these, 41% showed signs of neuropathy (N = 41), 53% used N2O tanks to fill balloons (N = 53), 71% used them frequently (N = 72), and 76% used them heavily (N = 77). We included 75 patients in the prospective study and 10 (13%) completed the first survey. All 10 patients fulfilled the criteria for SA and SD (DSM-IV-TR, median number of questions answered “yes” = 10/12), all used N2O tanks to fill balloons, and 90% (N = 9) experienced signs of neuropathy. After 1 and 3 months, 6/7 and 1/1 patients, respectively, continued to fulfill SA and SD criteria. Translating to DSM-V criteria, 1/10 patients fulfilled the criteria for (self-reported) mild SUD, 1/10 patients for moderate SUD, and 8/10 patients for severe SUD, 1 week after consultation. Conclusion: The high proportion of N2O-intoxicated patients reporting frequent and heavy use of N2O indicates an addictive potential of N2O. Although follow-up rate was low, all patients fulfilled self-reported SA, SD (DSM-IV-TR), and SUD (DSM-V) criteria for N2O. Somatic healthcare professionals treating patients with N2O intoxications should be aware of possible addictive behavior in patients. The screening, brief intervention, and referral to treatment approach should be considered to treat patients with self-reported SUD symptoms.
Interleukin-33 as a Biomarker Affecting Intrathecal Synthesis of Immunoglobulin in Neuromyelitis Optica Spectrum Disorder and Myelin Oligodendrocyte Glycoprotein Antibody-Associated Disease
Authors: Wang Mengyu , Xia Dongxia , Sun Lin , Bi Jianzhong , Xie Keqin , Wang Pin
Published: April 2023

Introduction: The purpose of this study was to analyze IL-33 maybe as a biomarker especially with respect to intrathecal immunoglobulin G (IgG) synthesis which was involved in the immune-mediated process in the demyelinating disease of the central nervous system. Methods: We aimed to determine the risk association of the serum and CSF levels of IL-33 in aquaporin-4 (AQP4)+neuromyelitis optica spectrum disorder (NMOSD) patients and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) patients compared with the control group. Levels of inflammatory (IL-2, IL-4, IL-6, and IL-10) markers and QAlb, the IgG index, and 24-h IgG synthesis rate were assessed in 28 AQP4+NMOSD patients and 11 MOGAD patients. Disease severity was assessed using the Expanded Disability Status Scale (EDSS). Results: The level of IL-33 in serum decreased first but then increased gradually in AQP4+NMOSD and MOGAD. The serum level of IL-2, IL-4, and IL-10 increased more significantly and decreased more rapidly after methylprednisolone treatment. The level of IL-33 in CSF increased progressively in AQP4+NMOSD and MOGAD, especially in MOGAD. The QAlb levels were increased significantly in the CSF of MOGAD patients and AQP4+NMOSD patients on the acute stage of the disease. The IgG index and 24-h IgG synthesis rate were also increased significantly in the CSF of two groups similarly. Conclusions: Thus, we concluded that IL-33 may induce dysfunction of the blood-brain barrier and lead to intrathecal synthesis of immunoglobulin in the AQP4+NMOSD and MOGAD, especially in MOGAD. It maybe as a biomarker, at least in part, was involved in the demyelinating diseases of the central nervous system.
Dupilumab-Associated Psoriasis and Psoriasiform Manifestations: A Scoping Review
Authors: Su Zheng , Zeng Yue-Ping
Published: April 2023

Background: Dupilumab is the first approved IL-4Rα inhibitor for the treatment of atopic dermatitis at present with good efficacy and safety. However, there have been several reports of psoriasis and psoriasiform manifestations occurring after dupilumab therapy in recent years, showing a new paradoxical cutaneous reaction associated with biologics. Summary: This is a scoping review in order to summarize the demographics and epidemiology, clinical manifestations, diagnosis, potential pathogenesis, and promising management of dupilumab-associated psoriasis and psoriasiform manifestations.
A Common Variant of ARRB2 Promoter Region Associated with the Prognosis of Heart Failure
Authors: Ren Hongqiang , liu Yijun , Tan Zhen , Luo Guiquan , Zhang Mei , Li Shuang , Tang Tingwei , Zhao Li
Published: April 2023

Introduction: The role of ARRB2 in cardiovascular disease has recently gained increasing attention. However, the association between ARRB2 polymorphisms and heart failure (HF) has not yet been investigated. Methods: A total of 2,386 hospitalized patients with chronic HF were enrolled as the first cohort and followed up for a mean period of 20.2 months. Meanwhile, ethnically and geographically matched 3,000 individuals without evidence of HF were included as healthy controls. We genotyped the common variant in ARRB2 gene to identify the association between variant and HF. A replicated independent cohort enrolling 837 patients with chronic HF was applied to validate the observed association. A series of function analyses were conducted to illuminate the underlying mechanism. Results: We identified a common variant rs75428611 associated with the prognosis of HF in two-stage population: adjusted p = 0.001, hazard ratio (HR) = 1.31 (1.11–1.54) in additive model and adjusted p = 0.001, HR = 1.39 (1.14–1.69) in dominant model in first-stage population; adjusted p = 0.04, HR = 1.41 (1.02–1.95) in additive model and adjusted p = 0.03, HR = 1.51 (1.03–2.20) in dominant model in replicated stage. However, rs75428611 did not significantly associate with the risk of HF. Functional analysis indicated that rs75428611‐G allele increased the promoter activity and the mRNA expression level of ARRB2 by facilitating transcription factor SRF binding but not the A allele. Conclusions: Our findings demonstrated that rs75428611 in promoter of ARRB2 was associated with the risk of HF mortality. It is a promising potential treatment target for HF.
Inventory and abundance of non-volant mammals and birds in the unprotected regions of the Mount Apo Range, Philippines
Authors: Villegas Jhonnel P. , Rosales Jireh R. , Tampos Giovanne G. , Ibañez Jayson C.
Published: April 2023

Wildlife, such as non-volant mammals and birds, play a vital role in the maintenance of ecosystem health. They are considered ecological engineers that influence forest vegetation. However, due to deforestation, habitat loss, and human persecution, its population status has declined over the years. This study aimed to conduct a species inventory and assess the relative abundance of non-volant mammals and birds in the unprotected regions of the Mt. Apo Range, Philippines, through camera trapping methods. Furthermore, the anthropogenic threats observed in the study areas were also documented. A total of 1,106 camera trap days were carried out in 2016 and another 500 days in 2020. Based on 260 independent sequences for both the 2016 and 2020 surveys, 12 species were identified, consisting of eight non-volant mammals and four birds. Among the identified species are the Endangered Philippine Brown Deer Rusa marianna & Philippine Long-tailed Macaque Macaca fascicularis philippensis and the Vulnerable Giant Scops-owl Otus gurneyi & the Philippine Warty Pig Sus philippensis. Video evidence of the Philippine Warty Pig Sus philippensis performing an important ecological role as an ecological engineer in the Philippine tropical forests were also captured for the first time. Another 61 independent sequences of unidentified rodents were detected in the camera traps, requiring further species monitoring techniques. Conservation must be strengthened beyond the protected landscapes of the Mt. Apo Range through community-based forest governance. This will ensure that the forest vertebrates are protected and conserved from further anthropogenic pressures.
Identification, prioritization, and management of biodiversity hot specks: a case study of Western Ghats of Maharashtra, India
Authors: Trivedi Shivam , Bharucha Erach
Published: April 2023

The Western Ghats are globally recognized as a hotspot of rich, endemic, and threatened biodiversity. Within this hotspot of biological diversity, there are islands of natural landscapes that can be termed as ‘hot specks’. These hot specks require careful prioritization and specific management strategies as they vary in objectives and ownership. Conserving hot specks of biodiversity is of great relevance because creating new protected areas with wildlife corridors between them has become relatively impossible in the present context of intensive land-use change in this rapidly developing region. Management strategies, however, must be based on scientific assessment and using a set of prioritization criteria for selecting the most appropriate forms of management. The conservation action plan for the Western Ghats has become a controversial issue based on the findings in the report submitted by the Western Ghats Expert Ecological Panel and the High Level Work Group on Western Ghats. In the present context of rapidly changing land-use patterns, economic development, forest fragmentation, isolation of habitats, linear intrusion, neo-urbanization and industrial growth are threats to the pristine nature of the ghats. Thus, there is an urgent need to identify, prioritize and manage the smaller fragments of biological importance within the larger ecologically sensitive landscape. A prioritization model for different types of hot specks is essential so that it can be easily replicated by training frontline forest staff, community-based organizations, Biodiversity Management Committees, and non-government organizations for implementing a strategy and action plans for the sites by using the Biological Diversity Act, 2002. Support of the local Biodiversity Management Committees and the State Biodiversity Board is essential for the conservation management of these biodiversity-rich sites. This study presents an innovative approach to prioritize areas outside the formally notified boundaries of the national parks and wildlife sanctuaries to assess the conservation value of hot specks of diversity through a rapid biodiversity assessment tool. This can lead to a rational conservation strategy that conservation planners and practitioners can use.
Japan’s Space Diplomacy in a World of Great Power Competition
Authors: Pekkanen Saadia M.
Published: April 2023

This article probes Japan’s space diplomacy in a world returned to great power competition. Today, Japan’s space diplomacy manifests in a wide variety of contexts at the global, regional and bilateral levels. The principal argument is that the Japanese state is responding to gathering external threats through diplomacy that communicates, persuades and bargains in the country’s science, economic and military space interests. This proactive positioning allows Japan to situate its advanced technologies in the context of fierce economic competition, cultivate an image as a pacifist and responsible actor in the turbulent international order, and set pragmatic expectations about building peaceful prospects in outer space.
Agricultural Prices in Scotland 1826–1974: County Level Data
Authors: Sawkins John W. , Smith Eric P.
Published: April 2023

This article presents a dataset comprising Scottish agricultural prices for the period 1826–1974. The data are derived from an archived source in which prices set at meetings of courts held across each of Scotland’s 32 historic counties were recorded. The courts, convened by county sheriffs, met once a year to gather evidence relating to county-wide prices for crops grown within each locality. Once set, the prices were used, primarily, to determine the annual stipends of the clergy of the established Church of Scotland. Prices varied according to crop variety and quality, with some counties setting up to three prices in any one year for one type of grain. The most widely grown crop was oatmeal, with wheat, peas and barley also extensively farmed. The system operated without interruption until the abolition of the ‘fiars’ courts in 1974. The dataset opens two key areas of research. First it enables the further development of analyses of the integration and operation of Scottish regional grain markets in the nineteenth and early twentieth centuries. Second it facilitates empirical analyses of the operation of the clerical labour market in Scotland for which long runs of stipend (wage) data collected on a consistent basis are required.
Understanding ‘Secularism’
Authors: Abbink Jon
Published: April 2023

Review article on Jacques Berlinerblau's Secularism: the Basics.
Optimization design of variable stiffness composite cylinders based on non-uniform rational B-spline curve/surface
Authors: Li Junxiang , Chen Zhiqiang , Chen Jianqiao , Song Qinghua
Published: April 2023

Compared with a constant stiffness (CS) composite structure of traditional straight fiber path, a variable stiffness (VS) composite structure of curvilinear fiber path has a broader design space. Taking the performance of a CS cylinder as the baseline, two curved fiber formats of VS cylinders are investigated. One is that the fiber angle varies in circumferential direction (i.e., circumferential stiffness variation, VS-C), the other is that the fiber angle varies in both circumferential and axial directions (i.e., circumferential and axial stiffness variation,VS-CA). Non-uniform rational B-spline curve/surface is used to define the continuously varying fiber orientation angle, making the VS design come true. This paper focuses on the maximum buckling capacity of VS composite cylinders under two kinds of loading cases (pure bending moment at both ends, concentrated force at the middle of the axis), and the optimal fiber angles of VS-C and VS-CA cylinders are obtained. In order to improve the computational efficiency, the adaptive Kriging surrogate model is integrated with genetic algorithm to solve the above optimization problems. The results show that the anti-buckling performance of VS-CA structure is always better or close to VS-C structure under different loading conditions, and all are better than that of CS structure.
Resistance and Gendered Racism: Middle-Class Black Women's Experiences Navigating Reproductive Health Care Systems
Authors: Howell Frances M.
Published: April 2023

A core issue in reproductive justice concerns how racism impacts reproductive health outcomes for Black women. However, the intersectional experiences of middle-class Black women navigating racism in reproductive health systems have not been fully captured in psychological literature. By utilizing a Black feminist approach to qualitative inquiry, this study situates the psychological dynamics behind middle-class Black women's interpretations of and reactions to gendered racism in reproductive settings within the historical context of slavery and its aftermath. The data analysis of 12 interviews captures how middle-class Black women reflect on their interactions with gynecologists, narrate their anticipation of gendered racism, and interpret and respond to experiencing gendered racism. Findings from this study suggest that gendered racism is a haunting of embodied gynecological trauma that maps onto the historical legacy of slavery. This study offers psychology an empirical and analytical framework for moving forward with its conceptualizations of how race, gender, and class intersect in service of reproductive justice. Results from this study can be used by clinicians to guide their clients towards healing gendered-racist-related stress, as well as medical schools to educate obstetricians and gynecologists on how to provide anti-racist care to their Black patients. Online slides for instructors who want to use this article for teaching are available on PWQ's website at http://journals.sagepub.com/doi/suppl/10.1177/03616843231168113 .
Fuzzy-interval approach and sensitivity analysis to assess dimensional tolerances of parallel manipulators
Authors: Lara-Molina Fabian Andres , Dumur Didier
Published: April 2023

This paper proposes an alternative approach based on a fuzzy-interval analysis to assess how dimensional tolerances affect the kinematics of parallel manipulators. The novel contribution of the proposed approach consists of modeling and analyzing the dimensional tolerances as fuzzy-intervals based on fuzzy theory and interval sensitivity methods. The dimensional tolerances are modeled as intervals weighted by the membership function for the proposed fuzzy-interval approach. The proposed fuzzy-interval analysis is used to analyze the positioning error, sensitivity, and design analysis. The proposed fuzzy-interval method was applied using numerical simulations to assess the effect of dimensional tolerances and sensitivity of parallel manipulators. The proposed approach showed as a complementary procedure that can be used in the design phase of parallel manipulators.
Using Walking / Go Along Interviews With People in Vulnerable Situations: A Synthesized Review of the Research Literature
Authors: Bartlett Ruth , Koncul Ana , Lid Inger Marie , George Elizabeth Onyedikachi , Haugen Ingebjørg
Published: April 2023

Walking/go along interviews are increasingly used in qualitative research studies to explore various phenomena, including the experience of disability. The method involves a researcher walking or travelling alongside a participant in their local neighborhood and asking questions along the way. It enables researchers to generate data about a person’s relationship with themselves and others, as well as the place in which they live. Scholars acknowledge that this method may pose risks and challenges for both participant and researcher, and so the rationale for using walking/go along interviews must be clarified. Further, qualitative researchers need to keep abreast of how the method is used with different participant groups, so methodological lessons can be shared within the research community. Therefore, the aim of this synthesized review was to identify, collate and analyse current evidence related to the use of walking/go along interviews with adults in a vulnerable situation, defined as those ‘at risk’ of discrimination, harm, or abuse due to disability and/or age. As such, the article combines, for the first time, the collective methodological knowledge of scholars working across disability, ageing, and dementia studies. The 23 articles included in the review were analyzed using the thematic synthesis method. Five themes were identified; (1) shifts in power dynamics (2) making things known and knowable (3) revealing barriers in the environment (4) embodied knowledge (of place), and (5) being one. An overall synthesis of these themes culminated in the methodological insight that walking interviews are broadly aligned with applied phenomenological research. Having studied how and why researchers deploy walking/go along interviews with people in a vulnerable situation, we conclude that it is a tool favored by scholars who wish to advance knowledge of the connection between micro-experiences, meso-movements, and macro-change.
Magnetohyperthermia-synergistic glioma cancer therapy enabled by magnetic graphene oxide nanoheaters: promising nanostructure for in vitro and in vivo applications
Authors: Sheervalilou Roghayeh , Khoei Samideh , Khoee Sepideh , Shirvaliloo Milad , Sadri Elaheh , Shirvalilou Sakine , Goudarzi Mina
Published: April 2023

In the present study, a magnetohyperthermia (MH)-based therapy is introduced as an effective treatment for deep-seated tumors especially glioma, which combines the magnetothermal effect and the reactive oxygen species (ROS) induced with magnetic graphene oxide (GOMNPs) nanoheaters to overcome the issue of limited therapeutic efficacy in the current magnetothermal therapeutic strategy. Magnetic graphene oxide nanoheaters with a size of 34 nm and a surface charge of − 35 mV showed very little toxicity under in vitro and ex vivo circumstances based on MTT and hemolysis assay, respectively. The application of GOMNPs under an alternating magnetic coil (AMC) showed that they had high specific absorption rate (SAR, P < 0.01), with enhanced level of ROS production within the tumor microenvironment. The results confirmed a significant increase in the SAR in blood compared to culture media (P < 0.01), which indicates the improvement of magnetohyperthermia performance in in vivo conditions compared to in vitro. A significant therapeutic efficacy was achieved with a dosage of 30 mg/kg of GOMNPs following 3 therapeutic courses under alternating magnetic field (AMF) compared to AMF alone (P < 0.05). GOMNPs exhibited a strong anti-glioma effect by inhibiting tumor growth, and increasing the survival ratio by inducing apoptotic cell death (P < 0.05). The Bax/Bcl2 protein and gene ratio confirmed a higher rate of apoptotic death in the MH-treated group (P < 0.05). Combination of magnetohyperthermia with conventional modalities may offer tremendous clinical advantages compared to the currently available methods.

Graphical Abstract
Magicmol: a light-weighted pipeline for drug-like molecule evolution and quick chemical space exploration
Authors: Chen Lin , Shen Qing , Lou Jungang
Published: April 2023

The flourishment of machine learning and deep learning methods has boosted the development of cheminformatics, especially regarding the application of drug discovery and new material exploration. Lower time and space expenses make it possible for scientists to search the enormous chemical space. Recently, some work combined reinforcement learning strategies with recurrent neural network (RNN)-based models to optimize the property of generated small molecules, which notably improved a batch of critical factors for these candidates. However, a common problem among these RNN-based methods is that several generated molecules have difficulty in synthesizing despite owning higher desired properties such as binding affinity. However, RNN-based framework better reproduces the molecule distribution among the training set than other categories of models during molecule exploration tasks. Thus, to optimize the whole exploration process and make it contribute to the optimization of specified molecules, we devised a light-weighted pipeline called Magicmol; this pipeline has a re-mastered RNN network and utilize SELFIES presentation instead of SMILES. Our backbone model achieved extraordinary performance while reducing the training cost; moreover, we devised reward truncate strategies to eliminate the model collapse problem. Additionally, adopting SELFIES presentation made it possible to combine STONED-SELFIES as a post-processing procedure for specified molecule optimization and quick chemical space exploration.
Genetic structure of an endangered species Ormosia henryi in southern China, and implications for conservation
Authors: Zhou Chengchuan , Xia Shiqi , Wen Qiang , Song Ying , Jia Quanquan , Wang Tian , Liu Liting , Ouyang Tianlin
Published: April 2023

Background

The evergreen broadleaved forest (EBLF) is an iconic vegetation type of East Asia, and it contributes fundamentally to biodiversity-based ecosystem functioning and services. However, the native habitat of EBLFs keeps on decreasing due to anthropogenic activities. Ormosia henryi is a valuable rare woody species in EBLFs that is particularly sensitive to habitat loss. In this study, ten natural populations of O. henryi in southern China were sampled, and then genotyping by sequencing (GBS) was applied to elucidate the standing genetic variation and population structure of this endangered species.

Results

In ten O. henryi populations, 64,158 high-quality SNPs were generated by GBS. Based on these markers, a relatively low level of genetic diversity was found with the expected heterozygosity (He) ranging from 0.2371 to 0.2901. Pairwise FST between populations varied from 0.0213 to 0.1652, indicating a moderate level of genetic differentiation. However, contemporary gene flow between populations were rare. Assignment test and principal component analysis (PCA) both supported that O. henryi populations in southern China could be divided into four genetic groups, and prominent genetic admixture was found in those populations located in southern Jiangxi Province. Mantel tests and multiple matrix regression with randomization (MMRR) analyses suggested that isolation by distance (IBD) could be the possible reason for describing the current population genetic structure. In addition, the effective population size (Ne) of O. henryi was extremely small, and showed a continuous declining trend since the Last Glacial Period.

Conclusions

Our results indicate that the endangered status of O. henryi is seriously underestimated. Artificial conservation measures should be applied as soon as possible to prevent O. henryi from the fate of extinction. Further studies are needed to elucidate the mechanism that leading to the continuous loss of genetic diversity in O. henryi and help to develop a better conservation strategy.
The utility of the Rapid Emergency Medicine Score (REMS) compared with three other early warning scores in predicting in-hospital mortality among COVID-19 patients in the emergency department: a multicenter validation study
Authors: Ruangsomboon Onlak , Phanprasert Nutthida , Jirathanavichai Supawich , Puchongmart Chanokporn , Boonmee Phetsinee , Thirawattanasoot Netiporn , Dorongthom Thawonrat , praphruetkit Nattakarn , Monsomboon Apichaya
Published: April 2023

Background

Many early warning scores (EWSs) have been validated to prognosticate adverse outcomes of COVID-19 in the Emergency Department (ED), including the quick Sequential Organ Failure Assessment (qSOFA), the Modified Early Warning Score (MEWS), and the National Early Warning Score (NEWS). However, the Rapid Emergency Medicine Score (REMS) has not been widely validated for this purpose. We aimed to assess and compare the prognostic utility of REMS with that of qSOFA, MEWS, and NEWS for predicting mortality in emergency COVID-19 patients.

Methods

We conducted a multi-center retrospective study at five EDs of various levels of care in Thailand. Adult patients visiting the ED who tested positive for COVID-19 prior to ED arrival or within the index hospital visit between January and December 2021 were included. Their EWSs at ED arrival were calculated and analysed. The primary outcome was all-cause in-hospital mortality. The secondary outcome was mechanical ventilation.

Results

A total of 978 patients were included in the study; 254 (26%) died at hospital discharge, and 155 (15.8%) were intubated. REMS yielded the highest discrimination capacity for in-hospital mortality (the area under the receiver operator characteristics curves (AUROC) 0.771 (95% confidence interval (CI) 0.738, 0.804)), which was significantly higher than qSOFA (AUROC 0.620 (95%CI 0.589, 0.651); p < 0.001), MEWS (AUROC 0.657 (95%CI 0.619, 0.694); p < 0.001), and NEWS (AUROC 0.732 (95%CI 0.697, 0.767); p = 0.037). REMS was also the best EWS in terms of calibration, overall model performance, and balanced diagnostic accuracy indices at its optimal cutoff. REMS also performed better than other EWSs for mechanical ventilation.

Conclusion

REMS was the early warning score with the highest prognostic utility as it outperformed qSOFA, MEWS, and NEWS in predicting in-hospital mortality in COVID-19 patients in the ED.
Outcomes of pregnant women exposed to Sotrovimab for the treatment of COVID-19 in the BA.1 Omicron predominant era (PRESTO)
Authors: Tuan Jessica J. , Sharma Manas , Kayani Jehanzeb , Davis Matthew W. , McManus Dayna , Topal Jeffrey E. , Ogbuagu Onyema
Published: April 2023

Background

Sotrovimab, a monoclonal antibody with efficacy against SARS-CoV-2 including certain Omicron variants, has been used in treatment of mild-moderate COVID-19. Limited data exists regarding its use in pregnant women.

Methods

Electronic medical record review of pregnant COVID-19 patients treated with sotrovimab from 12/30/21 − 1/31/22 (Yale New Haven Health Hospital System [YNHHS]) was performed. Included were pregnant individuals ≥ 12 years, weighing ≥ 40 kg, with positive SARS-CoV-2 test (within 10 days). Those receiving care outside YNHHS or receiving other SARS-CoV-2 treatment were excluded. We assessed demographics, medical history, and Monoclonal Antibody Screening Score (MASS). The primary composite clinical outcome assessed included emergency department (ED) visit < 24 h, hospitalization, intensive care unit (ICU) admission, and/or death within 29 days of sotrovimab. Secondarily, adverse feto-maternal outcomes and events for neonates were assessed at birth or through the end of the study period, which was 8/15/22.

Results

Among 22 subjects, median age was 32 years and body mass index was 27 kg/m2. 63% were Caucasian, 9% Hispanic, 14% African-American, and 9% Asian. 9% had diabetes and sickle cell disease. 5% had well-controlled HIV. 18%, 46%, and 36% received sotrovimab in trimester 1, 2, and 3, respectively. No infusion/allergic reactions occurred. MASS values were < 4. Only 12/22 (55%) received complete primary vaccination (46% mRNA-1273; 46% BNT162b2; 8% JNJ-78,436,735); none received a booster.

Conclusions

Pregnant COVID-19 patients receiving sotrovimab at our center tolerated it well with good clinical outcomes. Pregnancy and neonatal complications did not appear sotrovimab-related. Though a limited sample, our data helps elucidate the safety and tolerability of sotrovimab in pregnant women.
Intra-dialytic blood pressure variability is a greater predictor of cardiovascular events in hemodialysis patients
Authors: Liu Qixing , Wang Wei , Wu Xianglan , Lv Jiaxuan , Cai Shiming , Li Yuehong
Published: April 2023

Background

Short-term and long-term blood pressure variability (BPV) in hemodialysis (HD) population are risk factors of cardiovascular diseases (CVD) and all-cause mortality. There is no full consensus on the best BPV metric. We compared the prognostic role of intra-dialytic and visit-to-visit BPV metrics for CVD morbidity and all-cause mortality in HD patients.

Methods

A retrospective cohort of 120 patients on HD was followed up for 44 months. Systolic blood pressure (SBP) and baseline characteristics were collected for 3 months. We calculated intra-dialytic and visit-to-visit BPV metrics, including standard deviation (SD), coefficient of variation (CV), variability independent of the mean (VIM), average real variability (ARV) and residual. The primary outcomes were CVD events and all-cause mortality.

Results

In Cox regression analysis, both intra-dialytic and visit-to-visit BPV metrics were associated with increased CVD events (intra-dialytic CV: HR 1.70, 95% CI 1.28–2.27, p < 0.01; visit-to-visit CV: HR 1.55, 95% CI 1.12–2.16, p < 0.01), but not associated with increased all-cause mortality (intra-dialytic CV: HR 1.32, 95% CI 0.99–1.76, p = 0.06; visit-to-visit CV: HR 1.22, 95% CI 0.91–1.63, p = 0.18). Overall, intra-dialytic BPV showed greater prognostic ability than visit-to-visit BPV for both CVD event (AUC of intra-dialytic BPV and visit-to-visit BPV metrics respectively: SD 0.686, 0.606; CV 0.672, 0.425; VIM 0.677, 0.581; ARV 0.684, 0.618; residual 0.652, 0.586) and all-cause mortality (SD 0.671, 0.608; CV 0.662, 0.575; VIM 0.669, 0.581; ARV 0.529, 0.588; residual 0.651, 0.602).

Conclusion

Compared to visit-to-visit BPV, intra-dialytic BPV is a greater predictor of CVD event in HD patients. No obvious priority was found among various BPV metrics.
Comparison of endothelial cell loss in diabetic patients after conventional phacoemulsification and femtosecond laser-assisted cataract surgery
Authors: Cruz João Carlos Gonçalves , Moreno Celso Busnelo , Soares Paula Virginia Brom dos Santos , Moscovici Bernardo Kaplan , Colombo-Barboza Guilherme Novoa , Colombo-Barboza Luiz Roberto , Colombo-Barboza Marcello Novoa
Published: April 2023

Purpose

This study aims to comparatively evaluate the morphological changes of the cornea after phacoemulsification (PHACO) and femtosecond laser-assisted cataract surgery (FLACS) without intercurrences in patients with type 2 diabetes mellitus.

Methods

A total of 95 diabetic patients with moderate cataracts (N2 + and N3+), 47 undergoing PHACO and 48 undergoing FLACS, were selected randomly for the study. Surgeries were performed by a single surgeon between July 2021 and December 2021. Cumulative dissipated energy (CDE) and total balanced saline solution (BSS) data were obtained at the end of each surgery. Changes in corneal endothelial cell density (ECD) and central corneal thickness (CCT) at three months postoperatively were investigated.

Results

After three months, evidence is lacking between groups in the CCT measures; the difference was neither statistically nor clinically relevant. However, for ECD, a significant and clinically significant difference was found; if all patients were treated with laser, the mean ECD would be 423.55 greater (RSE: 86.09; p-value < 0.001; 95% CI: 254.81–592.29) than the ECD potential means of 1656.423 among the conventional group (RSE: 74.90; p-value < 0.001; 95% CI: 1509.62–1803.23).

Conclusions

Diabetic patients under treatment with moderate cataracts may predispose themselves to a more significant loss of endothelial cells after conventional phacoemulsification than femtosecond laser-assisted cataract surgery.

Trial registration

It was registered at The Brazilian Registry of Clinical Trials (ReBEC) with the code RBR-6d8whb5 (UTN code: U1111-1277-6020) on 17/05/2022.
The potential of tRF-21-U0EZY9X1B plasmatic level as a biomarker of children with obstructive sleep apnea-hypopnea syndrome
Authors: Lu Yanbo , Fu Qiang , Cai Xiaohong , Shen Yijing , Wu Junhua , Qiu Haiyan
Published: April 2023

Purpose

We investigated changes in plasma transfer RNA related fragments (tRF) in children with obstructive sleep apnea–hypopnea syndrome (OSAHS) and the potential value as a disease marker.

Methods

Firstly, we randomly selected five plasma samples from the case group and the control group for high-throughput RNA sequencing. Secondly, we screened one tRF with different expression between the two groups, amplified it by quantitative reverse transcription-PCR (qRT-PCR) and sequenced the amplified product. After confirming that the qRT-PCR results were consistent with the sequencing results and the sequence of the amplified product contained the original sequence of the tRF, we performed qRT-PCR on all samples. Then we analyzed the diagnostic value of the tRF and its correlation with some clinical data.

Results

A total of 50 OSAHS children and 38 control children were included in this study. There were significant differences in height, serum creatinine (SCR) and total cholesterol (TC) between the two groups. The plasma expression levels of tRF-21-U0EZY9X1B (tRF-21) were significantly different between the two groups. Receiver operating characteristic curve (ROC) showed that it had valuable diagnostic index, with area under the curve (AUC) of 0.773, 86.71% and 63.16% sensitivity and specificity.

Conclusions

The expression levels of tRF-21 in the plasma of OSAHS children decreased significantly which were closely related to hemoglobin, mean corpuscular hemoglobin, triglyceride and creatine kinase-MB, may become novel biomarkers for the diagnosis of pediatric OSAHS.
Pituitary adenoma consistency affects postoperative hormone function: a retrospective study
Authors: De Alcubierre Dario , Puliani Giulia , Cozzolino Alessia , Hasenmajer Valeria , Minnetti Marianna , Sada Valentina , Martines Valentina , Zaccagnino Antonella , Ruggeri Andrea Gennaro , Pofi Riccardo , Sbardella Emilia , Venneri Mary Anna
Published: April 2023

Background

Tumor consistency recently emerged as a key factor in surgical planning for pituitary adenomas, but its impact on postoperative endocrine function is still unclear. Our study aimed to evaluate the impact of tumor consistency on the development of postoperative pituitary deficiencies.

Methods

Single-center, retrospective analysis of consecutive pituitary surgeries performed between January 2017 and January 2021 at Policlinico Umberto I in Rome. All patients underwent radiological and biochemical evaluations at baseline, and hormone assessments 3 and 6 months after pituitary surgery. Postoperative MRI studies were used to determine resection rates following surgery. Data on tumor consistency, macroscopic appearance, neurosurgical approach, and intraoperative complications were collected.

Results

Fifty patients [24 women, mean age 57 ± 13 years, median tumor volume 4800 mm3 [95% CI 620–8828], were included. Greater tumor volume (χ2 = 14.621, p = 0.006) and male sex (χ2 = 12.178, p < 0.001) were associated with worse preoperative endocrine function. All patients underwent transsphenoidal adenomectomy. Fibrous consistency was observed in 10% of patients and was associated with a Ki-67 greater than 3% (χ2 = 8.154, p = 0.04), greater risk of developing postoperative hormone deficiencies (χ2 = 4.485, p = 0.05, OR = 8.571; 95% CI: 0.876–83.908), and lower resection rates (χ2 = 8.148, p = 0.004; OR 1.385, 95% CI; 1.040–1.844). Similarly, worse resection rates were observed in tumors with suprasellar extension (χ2 = 5.048, p = 0.02; OR = 6.000, 95% CI; 1.129–31.880) and CSI (χ2 = 4.000, p = 0.04; OR = 3.857, 95% CI; 0.997–14.916).

Conclusions

Tumor consistency might provide useful information about postoperative pituitary function, likely due to its impact on surgical procedures. Further prospective studies with larger cohorts are needed to confirm our preliminary findings.
The correlation of triglyceride/high-density lipoprotein cholesterol ratio with muscle mass in type 2 diabetes patients
Authors: Fu Qingsong , Zhang Zhenwen , Hu Wenchao , Yang Yinrong
Published: April 2023

Objective

Triglyceride/high-density lipoprotein cholesterol (TG/HDL-C) ratio is correlated with metabolic diseases. The prevalence of sarcopenia is significantly higher in type 2 diabetes mellitus (T2DM) patients compared with healthy controls. The purpose of our study is to evaluate the correlation of TG/HDL-C ratio with muscle mass in T2DM patients.

Method

Our study consists of 1048 T2DM inpatients recruited from the department of endocrinology. Skeletal muscle index (SMI) was detected with a dual energy X-ray absorptiometry method. Low muscle mass was diagnosed using the criteria of SMI less than 7.0 kg/m2 (in male subjects) or 5.4 kg/m2 (in female subjects).

Result

The prevalence of low muscle mass was 20.9% and 14.5% in male and female groups respectively. SMI was correlated with TG/HDL ratio after adjustment for age, duration of diabetes, diastolic blood pressure (DBP), and HbA1c in male subgroup. In female subgroup, SMI was associated with TG/HDL ratio after adjustment for age and DBP.

Conclusion

Higher TG/HDL-C ratio is correlated with muscle mass in T2DM patients.
Oral health status of students with visual or hearing impairments in Northeast China
Authors: Li Jian , Zhang Kaiqiang , Cha Chang , Lu Zhenfu , Liu Lu
Published: April 2023

Background

Visual or hearing impairments in students seriously affect their quality of life. The aim of this study was to identify oral hygiene status and its influencing factors on visual or hearing impairments in students in Northeast China.

Methods

This study was conducted in May 2022. A total of 118 visually impaired students and 56 hearing impaired students from Northeast China were included in this study via census. Oral examinations and questionnaire-based surveys of students and their teachers were conducted. The oral examinations included caries experience, prevalence of gingival bleeding and dental calculus. The questionnaires included three parts: Social demographics (residence, sex and race) and parents’ educational level; Oral hygiene habits and medical treatment behaviors; Knowledge and attitudes towards oral health care. This questionnaire was selected from the Fourth China National Oral Health Survey and the reliability and validity of the questionnaire were previously tested. T tests, one-way ANOVA, χ2 tests and multivariate logistic analyses were conducted to evaluate the differences and dependent variables of dental caries.

Results

The prevalence of dental caries in visually impaired and hearing impaired students were 66.10% and 66.07%. The mean number of DMFT, prevalence of gingival bleeding and dental calculus in visually impaired students were 2.71 ± 3.06, 52.08% and 59.38%, respectively. The mean number of DMFT, prevalence of gingival bleeding and dental calculus in hearing impaired students were 2.57 ± 2.83, 17.86% and 42.86%, respectively. The results of the multivariate logistic analysis showed that fluoride use and parents’ educational background had an impact on the caries experience of visually impaired students. The daily toothbrushing frequency and parents’ educational background had an impact on the caries experience of hearing impaired students.

Conclusions

The oral health situation of students with visual or hearing impairments remains severe. It is still necessary to promote oral and general health in this population.