bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Digestate Modified with Amendments on Soil Health and Plant Biomass under Varying Experimental Durations
Authors: Holatko Jiri , Brtnicky Martin , Mustafa Adnan , Kintl Antonin , Skarpa Petr , Ryant Pavel , Baltazar Tivadar , Malicek Ondrej , Latal Oldrich , Hammerschmiedt Tereza
Published: January 2023

A digestate with amendments provides plants with available nutrients and improves the microbiological properties of treated soil. Modification of a digestate through the addition of a biochar and sulphur source is less well-known. This pot experiment aimed at comparing the short- and long-time fertilization effects of a digestate enriched with biochar, with elemental sulphur, or with a combination of both on soil health and plant biomass. The experiment was carried out with maize, cultivated twice (1st–12th week = pre-cultivation; re-sowing after shoot harvest, 13th–24th = main cultivation) in soil amended with prepared digestate. The digestate used in pre-cultivation was incubated untreated (D) and was then treated with biochar (D + B), with elemental sulphur at a low (LS) and high (HS) dose, or with a combination of both (D + B + LS and D + B + HS). An additional unamended digestate (D) was added to each soil variant before the main cultivation. The application of digestate with a high dose of elemental sulphur and biochar mediated the most significant differences in the soil. The increase (compared to the unamended soil) was of short-term type (+11% and +6% increased total nitrogen and carbon after 12 weeks), then of long-term type (+54% and +30% increased sulphur and arylsulfatase activity after 24 weeks), and later emerged in the 13th to the 24th week of the experiment (+57% and +32% non-inhibited urease, increased N-acetyl-β-D-glucosaminidase and phosphatase). No significant differences in the effect of the applied amendments on dry aboveground plant biomass were observed.
Evaluation of the Influence of Grain Sizes of Nanostructured WO3 Ceramics on the Resistance to Radiation-Induced Softening
Authors: Kadyrzhanov Dauren B. , Kozlovskiy Artem L. , Zdorovets Maxim V. , Kenzhina Inesh E. , Shlimas Dmitriy I.
Published: January 2023

The main purpose of this study is to test a hypothesis about the effect of grain size on the resistance to destruction and changes in the strength and mechanical properties of oxide ceramics subjected to irradiation. WO3 powders were chosen as objects of study, which have a number of unique properties that meet the requirements for their use as a basis for inert matrices of dispersed nuclear fuel. The grain-size variation in WO3 ceramics was investigated by mechanochemical grinding of powders with different grinding speeds. Grinding conditions were experimentally selected to obtain powders with a high degree of size homogeneity, which were used for further research. During evaluation of the strength properties, it was found that a decrease in the grain size leads to an increase in the crack resistance, as well as the hardness of ceramics. The increase in strength properties can be explained by an increase in the dislocation density and the volume contribution of grain boundaries, which lead to hardening and an increase in resistance. During determination of the radiation damage resistance, it was found that a decrease in grain size to 50–70 nm leads to a decrease in the degree of radiation damage and the preservation of the resistance of irradiated ceramics to destruction and cracking.
Reduction of Radar Cross Section by Adopting Symmetrical Coding Metamaterial Design for Terahertz Frequency Applications
Authors: Ramachandran Tayaallen , Faruque Mohammad Rashed Iqbal , Singh Mandeep Singh Jit , Khandaker Mayeen Uddin , Salman Mohammad , Youssef Ahmed A. F.
Published: January 2023

This work focused on the novel and compact 1-bit symmetrical coding-based metamaterial for radar cross section reduction in terahertz frequencies. A couple of coding particles were constructed to impersonate the elements ‘0′ and ‘1′, which have phase differences of 180°. All the analytical simulations were performed by adopting Computer Simulation Technology Microwave Studio 2019 software. Moreover, the transmission coefficient of the element ‘1′ was examined as well by adopting similar software and validated by a high-frequency structure simulator. Meanwhile, the frequency range from 0 to 3 THz was set in this work. The phase response properties of each element were examined before constructing various coding metamaterial designs in smaller and bigger lattices. The proposed unit cells exhibit phase responses at 0.84 THz and 1.54 THz, respectively. Meanwhile, the analysis of various coding sequences was carried out and they manifest interesting monostatic and bistatic radar cross section (RCS) reduction performances. The Coding Sequence 2 manifests the best bistatic RCS reduction values in smaller lattices, which reduced from −69.8 dBm2 to −65.5 dBm2 at 1.54 THz. On the other hand, the monostatic RCS values for all lattices have an inclined line until they reach a frequency of 1.0 THz from more than −60 dBm2. However, from the 1.0 THz to 3.0 THz frequency range the RCS values have moderate discrepancies among the horizontal line for each lattice. Furthermore, two parametric studies were performed to examine the RCS reduction behaviour, for instance, multi-layer structures and as well tilt positioning of the proposed coding metamaterial. Overall it indicates that the integration of coding-based metamaterial successfully reduced the RCS values.
Music Therapy in Global Aphasia: A Case Report
Authors: Piccolo Adriana , Corallo Francesco , Cardile Davide , Torrisi Michele , Smorto Chiara , Cammaroto Simona , Lo Buono Viviana
Published: January 2023

Patients affected by global aphasia are no longer able to understand, produce, name objects, write and read. It occurs as a result of functional damage of ischemic or hemorrhagic origin affecting the entire peri-silvan region and frontal operculum. Rehabilitation training aims to promote an early intervention in the acute phase. We described a case of a 57-year-old female patient with left intraparenchymal fronto-temporo-parietal cerebral hemorrhage and right hemiplegia. After admission to clinical rehabilitative center, the patient was not able to perform simple orders and she presented a severe impairment of auditory and written comprehension. Eloquence was characterized by stereotypical emission of monosyllabic sounds and showed compromised praxis-constructive abilities. Rehabilitation included a program of Neurologic Music Therapy (NMT), specifically Symbolic Communication Training Through Music (SYCOM) and Musical Speech Stimulation (MUSTIM). Rehabilitative treatment was measured by improved cognitive and language performance of the patient from T0 to T1. Music rehabilitative interventions and continuous speech therapy improve visual attention and communicative intentionality. In order to confirm the effectiveness of data presented, further extensive studies of the sample would be necessary, to assess the real role of music therapy in post-stroke global aphasia.
Enhanced Productivity of Bottom-Blowing Copper-Smelting Process Using Plume Eye
Authors: Liao Jinfa , Tan Keqin , Zhao Baojun
Published: January 2023

Bottom-blowing copper smelting is a bath smelting technology recently developed in China. It has the advantages of good adaptability of raw materials, high oxygen utilization and thermal efficiency, and flexible production capacity. Plume eye is a unique phenomenon observed in the bottom-blowing copper-smelting furnace where the slag on the surface of the bath is pushed away by the high-pressure gas injected from the bottom. The existence of plume eye was first confirmed by analyzing the quenched industrial samples collected above the gas injection area and then investigated by laboratory water model experiments. Combining the plant operating data and the smelting mechanism of the copper concentrate, the role of the plume eye in bottom-blowing-enhanced smelting is analyzed. It reveals that the direct dissolution of copper concentrate as a low-grade matte into the molten matte can significantly accelerate the reactions between the concentrate and oxygen. The productivity of the bottom-blowing furnace is therefore increased as a result. The effects of the gas flow rate and thickness of the matte and of the slag layer on the diameter of the plume eye were studied using water-model experiments. It was found that increasing the gas flow and the thickness of the matte and reducing the thickness of the slag can increase the diameter of the plume eye. This work is of great significance for further understanding the copper bottom-blowing smelting technology and optimizing industrial operations.
Neonatal Orally Administered Zingerone Attenuates Alcohol-Induced Fatty Liver Disease in Experimental Rat Models
Authors: Asiedu Bernice , Lembede Busisani Wiseman , Gomes Monica , Kasonga Abe , Nkomozepi Pilani , Nyakudya Trevor Tapiwa , Chivandi Eliton
Published: January 2023

Alcohol intake at different developmental stages can lead to the development of alcohol-induced fatty liver disease (AFLD). Zingerone (ZO) possess hepato-protective properties; thus, when administered neonatally, it could render protection against AFLD. This study aimed to evaluate the potential long-term protective effect of ZO against the development of AFLD. One hundred and twenty-three 10-day-old Sprague–Dawley rat pups (60 males; 63 females) were randomly assigned to four groups and orally administered the following treatment regimens daily during the pre-weaning period from postnatal day (PND) 12–21: group 1—nutritive milk (NM), group 2—NM +1 g/kg ethanol (Eth), group 3—NM + 40 mg/kg ZO, group 4—NM + Eth +ZO. From PND 46–100, each group from the neonatal stage was divided into two; subgroup I had tap water and subgroup II had ethanol solution as drinking fluid, respectively, for eight weeks. Mean daily ethanol intake, which ranged from 10 to 14.5 g/kg body mass/day, resulted in significant CYP2E1 elevation (p < 0.05). Both late single hit and double hit with alcohol increased liver fat content, caused hepatic macrosteatosis, dysregulated mRNA expression of SREBP1c and PPAR-α in male and female rats (p < 0.05). However, neonatal orally administered ZO protected against liver lipid accretion and SREBP1c upregulation in male rats only and attenuated the alcohol-induced hepatic PPAR-α downregulation and macrosteatosis in both sexes. This data suggests that neonatal orally administered zingerone can be a potential prophylactic agent against the development of AFLD.
Study the Effect of Relative Energy Deficiency on Physiological and Physical Variables in Professional Women Athletes: A Randomized Controlled Trial
Authors: Miralles-Amorós Laura , Asencio-Mas Nuria , Martínez-Olcina María , Vicente-Martínez Manuel , Frutos José Manuel García-De , Peñaranda-Moraga Marcelo , Gonzálvez-Alvarado Lucía , Yáñez-Sepúlveda Rodrigo , Cortés-Roco Guillermo , Martínez-Rodríguez Alejandro
Published: January 2023

Energy deficits are often observed in athletes, especially in female athletes, due to the high expenditure of sport and strict diets. Low energy availability can cause serious health problems and affect sport performance. The aim of this study was to evaluate the effects of different personalized dietary plans on physiological and physical factors related to energy deficit syndrome in female professional handball players. Twenty-one professional female handball players, aged 22 ± 4 years, 172.0 ± 5.4 cm and 68.4 ± 6.7 kg, divided into three groups (FD: free diet; MD: Mediterranean diet; and AD: high antioxidant diet), participated in this 12-week randomized controlled trial. Energy expenditure through indirect calorimetry, energy availability, 7 day dietary intake analysis, blood pressure, cholesterol, menstrual function, body composition by both anthropometry and bioelectrical impedance, and strength performance were assessed. All participants showed low energy availability (<30 kcal/lean mass per day); despite this, all had eumenorrhea. Significant improvements were found after the intervention in all components of body composition (p < 0.05). In the remaining variables, despite slight improvements, none were significant neither over time nor between the different groups. Low energy availability has been observed in all professional female handball players, which may lead to serious consequences. A longer period of intervention is required to assess the differences between diets and improvements in other parameters.
The Status of Molecular Analyses of Isolates of Acanthamoeba Maintained by International Culture Collections
Authors: Fuerst Paul A.
Published: January 2023

Acanthamoeba is among the most ubiquitous protistan groups in nature. Knowledge of the biological diversity of Acanthamoeba comes in part from the use of strains maintained by the major microbial culture collections, ATCC and CCAP. Standard strains are vital to ensure the comparability of research. The diversity of standard strains of Acanthamoeba in the culture collections is reviewed, emphasizing the extent of genotypic studies based on DNA sequencing of the small subunit ribosomal RNA from the nucleus (18S rRNA gene; Rns) or the mitochondria (16S-like rRNA gene; rns). Over 170 different strains have been maintained at some time by culture centers. DNA sequence information is available for more than 70% of these strains. Determination of the genotypic classification of standard strains within the genus indicates that frequencies of types within culture collections only roughly mirror that from clinical or environmental studies, with significant differences in the frequency of some genotypes. Culture collections include the type of isolate from almost all named species of Acanthamoeba, allowing an evaluation of the validity of species designations. Multiple species are found to share the same Sequence Type, while multiple Sequence Types have been identified for different strains that share the same species name. Issues of sequence reliability and the possibility that a small number of standard strains have been mislabeled when studied are also examined, leading to potential problems for comparative analyses. It is important that all species have reliable genotype designations. The culture collections should be encouraged to assist in completing the molecular inventory of standard strains, while workers in the Acanthamoeba research community should endeavor to ensure that strains representative of genotypes that are missing from the culture collection are provided to the culture centers for preservation.
C-di-AMP Is a Second Messenger in Corynebacterium glutamicum That Regulates Expression of a Cell Wall-Related Peptidase via a Riboswitch
Authors: Reich Sebastian J. , Goldbeck Oliver , Lkhaasuren Tsenguunmaa , Weixler Dominik , Weiß Tamara , Eikmanns Bernhard J.
Published: January 2023

Cyclic di-adenosine monophosphate (c-di-AMP) is a bacterial second messenger discovered in Bacillus subtilis and involved in potassium homeostasis, cell wall maintenance and/or DNA stress response. As the role of c-di-AMP has been mostly studied in Firmicutes, we sought to increase the understanding of its role in Actinobacteria, namely in Corynebacterium glutamicum. This organism is a well-known industrial production host and a model organism for pathogens, such as C. diphtheriae or Mycobacterium tuberculosis. Here, we identify and analyze the minimal set of two C. glutamicum enzymes, the diadenylate cyclase DisA and the phosphodiesterase PdeA, responsible for c-di-AMP metabolism. DisA synthesizes c-di-AMP from two molecules of ATP, whereas PdeA degrades c-di-AMP, as well as the linear degradation intermediate phosphoadenylyl-(3′→5′)-adenosine (pApA) to two molecules of AMP. Here, we show that a ydaO/kimA-type c-di-AMP-dependent riboswitch controls the expression of the strictly regulated cell wall peptidase gene nlpC in C. glutamicum. In contrast to previously described members of the ydaO/kimA-type riboswitches, our results suggest that the C. glutamicum nlpC riboswitch likely affects the translation instead of the transcription of its downstream gene. Although strongly regulated by different mechanisms, we show that the absence of nlpC, the first known regulatory target of c-di-AMP in C. glutamicum, is not detrimental for this organism under the tested conditions.
Tailoring Heat Transfer and Bactericidal Response in Multifunctional Cotton Composites
Authors: Delgado Lilian Pérez , Franco-Bacca Adriana Paola , Cervantes-Alvarez Fernando , Ortiz-Vazquez Elizabeth , Ramon-Sierra Jesús Manuel , Rejon Victor , Aguirre-Macedo María Leopoldina , Alvarado-Gil Juan José , Rodríguez-Gattorno Geonel
Published: January 2023

Through the execution of scientific innovations, “smart materials” are shaping the future of technology by interacting and responding to changes in our environment. To make this a successful reality, proper component selection, synthesis procedures, and functional active agents must converge in practical and resource-efficient procedures to lay the foundations for a profitable and sustainable industry. Here we show how the reaction time, temperature, and surface stabilizer concentration impact the most promising functional properties in a cotton-based fabric coated with silver nanoparticles (AgNPs@cotton), i.e., the thermal and bactericidal response. The coating quality was characterized and linked to the selected synthesis parameters and correlated by a parallel description of “proof of concept” experiments for the differential heat transfer (conversion and dissipation properties) and the bactericidal response tested against reference bacteria and natural bacterial populations (from a beach, cenote, and swamp of the Yucatan Peninsula). The quantification of functional responses allowed us to establish the relationship between (i) the size and shape of the AgNPs, (ii) the collective response of their agglomerates, and (iii) the thermal barrier role of a surface modifier as PVP. The procedures and evaluations in this work enable a spectrum of synthesis coordinates that facilitate the formulation of application-modulated fabrics, with grounded examples reflected in “smart packaging”, “smart clothing”, and “smart dressing”.
Size, Morphology and Crystallinity Control Strategy of Ultrafine HMX by Microfluidic Platform
Authors: Jiang Hanyu , Wang Xuanjun , Yu Jin , Zhou Wenjun , Zhao Shuangfei , Xu Siyu , Zhao Fengqi
Published: January 2023

The crystal structure has a great influence on mechanical sensitivity and detonation performance of energetic materials. An efficient microfluidic platform was applied for size, morphology, and crystallinity controllable preparation of ultrafine HMX. The microfluidic platform has good mixing performance, quick response, and less reagent consumption. The ultrafine γ-HMX was first prepared at room temperature by microfluidic strategy, and the crystal type can be controlled accurately by adjusting the process parameters. With the increase in flow ratio, the particle size decreases gradually, and the crystal type changed from β-HMX to γ-HMX. Thermal behavior of ultrafine HMX shows that γ→δ is easier than β→δ, and the phase stability of HMX is β > γ > δ. Furthermore, the ultrafine β-HMX has higher thermal stability and energy release efficiency than that of raw HMX. The ultrafine HMX prepared by microfluidic not only has uniform morphology and narrow particle size distribution, but also exhibits high density and low sensitivity. This study provides a safe, facile, and efficient way of controlling particle size, morphology, and crystallinity of ultrafine HMX.
Finite Element Analysis Model of Electronic Skin Based on Surface Acoustic Wave Sensor
Authors: Jiao Chunxiao , Wang Chengkai , Wang Meng , Pan Jinghong , Gao Chao , Wang Qi
Published: January 2023

In recent years, with the rapid development of flexible electronic devices, researchers have a great interest in the research of electronic skin (e-skin). Traditional e-skin, which is made of rigid integrated circuit chips, not only limits the overall flexibility, but also consumes a lot of power and poses certain security risks to the human body. In this paper, a wireless passive e-skin is designed based on the surface acoustic wave sensor (SAWS) of lithium niobate piezoelectric film. The e-skin has the advantages of small size, high precision, low power consumption, and good flexibility. With the multi-sensing function of stress, temperature, and sweat ion concentration, etc., the newly designed e-skin is a sensor platform for a wide range of external stimuli, and the measurement results can be directly presented in frequency. In order to explore the characteristic parameters and various application scenarios of the SAWS, finite element analysis is carried out using the simulation software; the relationship between the SAWS and various influencing factors is explored, and the related performance curve is obtained. These simulation results provide important reference and experimental guidance for the design and preparation of SAW e-skin.
Unexplored Roles of Erythrocytes in Atherothrombotic Stroke
Authors: Papadopoulos Charalampos , Anagnostopoulos Konstantinos , Tsiptsios Dimitrios , Karatzetzou Stella , Liaptsi Eirini , Lazaridou Irene Zacharo , Kokkotis Christos , Makri Evangelia , Ioannidou Maria , Aggelousis Nikolaos , Vadikolias Konstantinos
Published: January 2023

Stroke constitutes the second highest cause of morbidity and mortality worldwide while also impacting the world economy, triggering substantial financial burden in national health systems. High levels of blood glucose, homocysteine, and cholesterol are causative factors for atherothrombosis. These molecules induce erythrocyte dysfunction, which can culminate in atherosclerosis, thrombosis, thrombus stabilization, and post-stroke hypoxia. Glucose, toxic lipids, and homocysteine result in erythrocyte oxidative stress. This leads to phosphatidylserine exposure, promoting phagocytosis. Phagocytosis by endothelial cells, intraplaque macrophages, and vascular smooth muscle cells contribute to the expansion of the atherosclerotic plaque. In addition, oxidative stress-induced erythrocytes and endothelial cell arginase upregulation limit the pool for nitric oxide synthesis, leading to endothelial activation. Increased arginase activity may also lead to the formation of polyamines, which limit the deformability of red blood cells, hence facilitating erythrophagocytosis. Erythrocytes can also participate in the activation of platelets through the release of ADP and ATP and the activation of death receptors and pro-thrombin. Damaged erythrocytes can also associate with neutrophil extracellular traps and subsequently activate T lymphocytes. In addition, reduced levels of CD47 protein in the surface of red blood cells can also lead to erythrophagocytosis and a reduced association with fibrinogen. In the ischemic tissue, impaired erythrocyte 2,3 biphosphoglycerate, because of obesity or aging, can also favor hypoxic brain inflammation, while the release of damage molecules can lead to further erythrocyte dysfunction and death.
The One-Hundred-Year Anniversary of the Discovery of the Sunshine Vitamin D3: Historical, Personal Experience and Evidence-Based Perspectives
Authors: Holick Michael F.
Published: January 2023

The discovery of a fat-soluble nutrient that had antirachitic activity and no vitamin A activity by McCollum has had far reaching health benefits for children and adults. He named this nutrient vitamin D. The goal of this review and personal experiences is to give the reader a broad perspective almost from the beginning of time for how vitamin D evolved to became intimately involved in the evolution of land vertebrates. It was the deficiency of sunlight causing the devastating skeletal disease known as English disease and rickets that provided the first insight as to the relationship of sunlight and the cutaneous production of vitamin D3. The initial appreciation that vitamin D could be obtained from ultraviolet exposure of ergosterol in yeast to produce vitamin D2 resulted in the fortification of foods with vitamin D2 and the eradication of rickets. Vitamin D3 and vitamin D2 (represented as D) are equally effective in humans. They undergo sequential metabolism to produce the active form of vitamin D, 1,25-dihydroxyvitamin D. It is now also recognized that essentially every tissue and cell in the body not only has a vitamin D receptor but can produce 1,25-dihydroxyvitamin D. This could explain why vitamin D deficiency has now been related to many acute and chronic illnesses, including COVID-19.
Osteocytic Sclerostin Expression as an Indicator of Altered Bone Turnover
Authors: Huybrechts Yentl , Evenepoel Pieter , Haarhaus Mathias , Cavalier Etienne , Dams Geert , Van Hul Wim , D’Haese Patrick C. , Verhulst Anja
Published: January 2023

Renal osteodystrophy (ROD) is a complex and serious complication of chronic kidney disease (CKD), a major global health problem caused by loss of renal function. Currently, the gold standard to accurately diagnose ROD is based on quantitative histomorphometric analysis of trabecular bone. Although this analysis encompasses the evaluation of osteoblast and osteoclast number/activity, tfigurehe interest in osteocytes remains almost nihil. Nevertheless, this cell type is evidenced to perform a key role in bone turnover, particularly through its production of various bone proteins, such as sclerostin. In this study, we aim to investigate, in the context of ROD, to which extent an association exists between bone turnover and the abundance of osteocytes and osteocytic sclerostin expression in both the trabecular and cortical bone compartments. Additionally, the effect of parathyroid hormone (PTH) on bone sclerostin expression was examined in parathyroidectomized rats. Our results indicate that PTH exerts a direct inhibitory function on sclerostin, which in turn negatively affects bone turnover and mineralization. Moreover, this study emphasizes the functional differences between cortical and trabecular bone, as the number of (sclerostin-positive) osteocytes is dependent on the respective bone compartment. Finally, we evaluated the potential of sclerostin as a marker for CKD and found that the diagnostic performance of circulating sclerostin is limited and that changes in skeletal sclerostin expression occur more rapidly and more pronounced. The inclusion of osteocytic sclerostin expression and cortical bone analysis could be relevant when performing bone histomorphometric analysis for diagnostic purposes and to unravel pathological mechanisms of bone disease.
A Focused Review on Cognitive Improvement by the Genus Salvia L. (Sage)—From Ethnopharmacology to Clinical Evidence
Authors: Ertas Abdulselam , Yigitkan Serkan , Orhan Ilkay Erdogan
Published: January 2023

Ethnopharmacology has been an important starting point in medical and pharmaceutical sciences for discovering drug candidates from natural sources. In this regard, the genus Salvia L., commonly known as sage, is one of the best-known medicinal and aromatic plants of the Lamiaceae family; it has been recorded as being used for memory enhancement in European folk medicine. Despite the various uses of sage in folk medicines, the records that have pointed out sage’s memory-enhancing properties have paved the way for the aforementioned effect to be proven on scientific grounds. There are many preclinical studies and excellent reviews referring to the favorable effect of different species of sage against the cognitive dysfunction that is related to Alzheimer’s disease (AD). Hence, the current review discusses clinical studies that provide evidence for the effect of Salvia species on cognitive dysfunction. Clinical studies have shown that some Salvia species, i.e., hydroalcoholic extracts and essential oils of S. officinalis L. and S. lavandulaefolia leaves in particular, have been the most prominently effective species in patients with mild to moderate AD, and these species have shown positive effects on the memory of young and healthy people. However, the numbers of subjects in the studies were small, and standardized extracts were not used for the most part. Our review points out to the need for longer-term clinical studies with higher numbers of subjects being administered standardized sage preparations.
Effects of Date Palm Waste Compost Application on Root Proteome Changes of Barley (Hordeum vulgare L.)
Authors: Ghouili Emna , Sassi Khaled , Hidri Yassine , M’Hamed Hatem , Somenahally Anil , Xue Qingwu , Jebara Moez , Nefissi Ouertani Rim , Riahi Jouhaina , de Oliveira Ana , Abid Ghassen , Muhovski Yordan
Published: January 2023

Proteomic analysis was performed to investigate the differentially abundant proteins (DAPs) in barley roots during the tillering stage. Bioinformatic tools were used to interpret the biological function, the pathway analysis and the visualisation of the network amongst the identified proteins. A total of 72 DAPs (33 upregulated and 39 downregulated) among a total of 2580 proteins were identified in response to compost treatment, suggesting multiple pathways of primary and secondary metabolism, such as carbohydrates and energy metabolism, phenylpropanoid pathway, glycolysis pathway, protein synthesis and degradation, redox homeostasis, RNA processing, stress response, cytoskeleton organisation, and phytohormone metabolic pathways. The expression of DAPs was further validated by qRT-PCR. The effects on barley plant development, such as the promotion of root growth and biomass increase, were associated with a change in energy metabolism and protein synthesis. The activation of enzymes involved in redox homeostasis and the regulation of stress response proteins suggest a protective effect of compost, consequently improving barley growth and stress acclimation through the reduction of the environmental impact of productive agriculture. Overall, these results may facilitate a better understanding of the molecular mechanism of compost-promoted plant growth and provide valuable information for the identification of critical genes/proteins in barley as potential targets of compost.
Analysis of the Effect of the Surface Inclination Angle on the Roughness of Polymeric Parts Obtained with Fused Filament Fabrication Technology
Authors: Martín Fernández Francisco , Martín Sánchez María Jesús
Published: January 2023

The aim of this work was to conduct a dimensional study, in terms of microgeometry, using parts from an additive manufacturing process with fused filament fabrication (FFF) technology. As in most cases of additive manufacturing processes, curved surfaces were obtained via approximation of planes with different inclinations. The focus of this experimental study was to analyze the surface roughness of curve geometry from surface-roughness measurements of the plane surfaces that generate it. Three relevant manufacturing parameters were considered: layer height, nozzle diameter and material. Taguchi’s experimental design based on the Latin square was applied to optimize the set of specimens used. For the manufactured samples, the surface-roughness parameters Ra (roughness average), Rq (root mean square roughness) and Rz (maximum height) were obtained in eight planes of different inclinations (0° to 90°). The results were analyzed using both a graphical model and an analysis of variance study (ANOVA), demonstrating the dependency relationships among the parameters considered and surface finish. The best surface roughness was reached at 85°, with a global average Ra value of 8.66 µm, increasing the average Ra value from 6.39 µm to 11.57 µm according to the layer height increase or decreasing it slightly, from 8.91 µm to 8.41 µm, in relation to the nozzle diameter increase. On the contrary, the worst surface roughness occurred at 20°, with a global average Ra value of 19.05 µm. Additionally, the theoretical profiles and those from the surface-roughness measurement were found to coincide greatly. Eventually, the eight regression curves from the ANOVA allowed prediction of outputs from future specimens tested under different conditions.
Accelerating Payload Release from Complex Coacervates through Mechanical Stimulation
Authors: Hatem Wesam A. , Lapitsky Yakov
Published: January 2023

Complex coacervates formed through the association of charged polymers with oppositely charged species are often investigated for controlled release applications and can provide highly sustained (multi-day, -week or -month) release of both small-molecule and macromolecular actives. This release, however, can sometimes be too slow to deliver the active molecules in the doses needed to achieve the desired effect. Here, we explore how the slow release of small molecules from coacervate matrices can be accelerated through mechanical stimulation. Using coacervates formed through the association of poly(allylamine hydrochloride) (PAH) with pentavalent tripolyphosphate (TPP) ions and Rhodamine B dye as the model coacervate and payload, we demonstrate that slow payload release from complex coacervates can be accelerated severalfold through mechanical stimulation (akin to flavor release from a chewed piece of gum). The stimulation leading to this effect can be readily achieved through either perforation (with needles) or compression of the coacervates and, besides accelerating the release, can result in a deswelling of the coacervate phases. The mechanical activation effect evidently reflects the rupture and collapse of solvent-filled pores, which form due to osmotic swelling of the solute-charged coacervate pellets and is most pronounced in release media that favor swelling. This stimulation effect is therefore strong in deionized water (where the swelling is substantial) and only subtle and shorter-lived in phosphate buffered saline (where the PAH/TPP coacervate swelling is inhibited). Taken together, these findings suggest that mechanical activation could be useful in extending the complex coacervate matrix efficacy in highly sustained release applications where the slowly releasing coacervate-based sustained release vehicles undergo significant osmotic swelling.
Modification of Polyvinyl Chloride Composites for Radiographic Detection of Polyvinyl Chloride Retained Surgical Items
Authors: Polaskova Martina , Sedlacek Tomas , Polasek Zdenek , Filip Petr
Published: January 2023

The ever-present risk of surgical items being retained represents a real medical peril for the patient and potential liability issues for medical staff. Radiofrequency scanning technology is a very good means to substantially reduce such accidents. Radiolucent medical-grade polyvinyl chloride (PVC) used for the production of medical items is filled with radiopaque agents to enable X-ray visibility. The present study proves the suitability of bismuth oxychloride (BiOCl) and documents its advantages over the classical radiopaque agent barium sulfate (BaSO4). An addition of BiOCl exhibits excellent chemical and physical stability (no leaching, thermo-mechanical properties) and good dispersibility within the PVC matrix. As documented, using half the quantity of BiOCl compared to BaSO4 will provide a very good result. The conclusions are based on the methods of rotational rheometry, scanning electron microscopy, dynamic mechanical analysis, atomic absorption spectroscopy, and the verification of zero leaching of BiOCl out of a PVC matrix. X-ray images of the studied materials are presented, and an optimal concentration of BiOCl is evaluated.
Characterisation of Banana Plant Growth Using High-Spatiotemporal-Resolution Multispectral UAV Imagery
Authors: Aeberli Aaron , Phinn Stuart , Johansen Kasper , Robson Andrew , Lamb David W.
Published: January 2023

The determination of key phenological growth stages of banana plantations, such as flower emergence and plant establishment, is difficult due to the asynchronous growth habit of banana plants. Identifying phenological events assists growers in determining plant maturity, and harvest timing and guides the application of time-specific crop inputs. Currently, phenological monitoring requires repeated manual observations of individual plants’ growth stages, which is highly laborious, time-inefficient, and requires the handling and integration of large field-based data sets. The ability of growers to accurately forecast yield is also compounded by the asynchronous growth of banana plants. Satellite remote sensing has proved effective in monitoring spatial and temporal crop phenology in many broadacre crops. However, for banana crops, very high spatial and temporal resolution imagery is required to enable individual plant level monitoring. Unoccupied aerial vehicle (UAV)-based sensing technologies provide a cost-effective solution, with the potential to derive information on health, yield, and growth in a timely, consistent, and quantifiable manner. Our research explores the ability of UAV-derived data to track temporal phenological changes of individual banana plants from follower establishment to harvest. Individual plant crowns were delineated using object-based image analysis, with calculations of canopy height and canopy area producing strong correlations against corresponding ground-based measures of these parameters (R2 of 0.77 and 0.69 respectively). A temporal profile of canopy reflectance and plant morphology for 15 selected banana plants were derived from UAV-captured multispectral data over 21 UAV campaigns. The temporal profile was validated against ground-based determinations of key phenological growth stages. Derived measures of minimum plant height provided the strongest correlations to plant establishment and harvest, whilst interpolated maxima of normalised difference vegetation index (NDVI) best indicated flower emergence. For pre-harvest yield forecasting, the Enhanced Vegetation Index 2 provided the strongest relationship (R2 = 0.77) from imagery captured near flower emergence. These findings demonstrate that UAV-based multitemporal crop monitoring of individual banana plants can be used to determine key growing stages of banana plants and offer pre-harvest yield forecasts.
Interior and Evolution of the Giant Planets
Authors: Miguel Yamila , Vazan Allona
Published: January 2023

The giant planets were the first to form and hold the key to unveiling the solar system’s formation history in their interiors and atmospheres. Furthermore, the unique conditions present in the interiors of the giant planets make them natural laboratories for exploring different elements under extreme conditions. We are at a unique time to study these planets. The missions Juno to Jupiter and Cassini to Saturn have provided invaluable information to reveal their interiors like never before, including extremely accurate gravity data, atmospheric abundances and magnetic field measurements that revolutionised our knowledge of their interior structures. At the same time, new laboratory experiments and modelling efforts also improved, and statistical analysis of these planets is now possible to explore all the different conditions that shape their interiors. We review the interior structure of Jupiter, Saturn, Uranus and Neptune, including the need for inhomogeneous structures to explain the data, the problems unsolved and the effect that advances in our understanding of their internal structure have on their formation and evolution.
Assessing Subsoil Conditions with an ASABE Conform Vertical Penetrometer—Development and Evaluation
Authors: Schmittmann Oliver , Lammers Peter Schulze
Published: January 2023

Soil is the habitat for soil organisms and associated soil physical and chemical processes. The subsoil is a large reserve of water and nutrients. Soil and subsoil are thus significantly involved in the yield capacity of a site and its resilience in the case of unfavorable weather conditions. Subsoil can also retain water in drought phases and stores carbon. In times of climate change and scarcity of resources, many scientific activities involve subsoil and require sensors to assess subsoil conditions and properties. An electrically driven penetrometer with an integrated soil water content sensor could be an appropriate tool for such applications; however, such a subsoil measurement tool does not exist. One major reason for this is that, when penetrating compacted subsoil, high penetration forces (including friction) act on the penetrating thin rod (diameter 1 cm). The development of a tractor-mounted subsoil penetrometer for depths up to 2 m is described in this study. An ASABE standard cone is implemented, which can access heavy compacted layers. The rod, which includes wires for embedding an FDI moisture sensor in the cone tip, is covered by a protection tube. The penetration resistance measurement can be performed without being influenced by shaft friction. The rod, along with the sensor, is implemented in a tower that can be shifted laterally and can take probes in a single line without moving the tractor. To confirm the quality of the developed subsoil penetrometer, a suitable evaluation method is presented. Typical arable soil (loamy silt) was filled in boxes and compacted homogeneously using a hydraulic stamp so that different setups of the penetrometer could be compared and evaluated. The evaluation concludes that the distance between the free cone tip and the protection shaft should be at least 10 cm to measure the penetration resistance of soil without being influenced by the protection tube. Furthermore, the developed penetrometer has sufficient stability and precision for accessing subsoil. In field trials, the subsoil penetrometer was compared with a standard penetrometer and has proved its suitability.
Low-Cost Technologies Used in Corrosion Monitoring
Authors: Komary Mahyad , Komarizadehasl Seyedmilad , Tošić Nikola , Segura I. , Lozano-Galant Jose Antonio , Turmo Jose
Published: January 2023

Globally, corrosion is the costliest cause of the deterioration of metallic and concrete structures, leading to significant financial losses and unexpected loss of life. Therefore, corrosion monitoring is vital to the assessment of structures’ residual performance and for the identification of pathologies in early stages for the predictive maintenance of facilities. However, the high price tag on available corrosion monitoring systems leads to their exclusive use for structural health monitoring applications, especially for atmospheric corrosion detection in civil structures. In this paper a systematic literature review is provided on the state-of-the-art electrochemical methods and physical methods used so far for corrosion monitoring compatible with low-cost sensors and data acquisition devices for metallic and concrete structures. In addition, special attention is paid to the use of these devices for corrosion monitoring and detection for in situ applications in different industries. This analysis demonstrates the possible applications of low-cost sensors in the corrosion monitoring sector. In addition, this study provides scholars with preferred techniques and the most common microcontrollers, such as Arduino, to overcome the corrosion monitoring difficulties in the construction industry.
A Novel Deep Learning Approach to 5G CSI/Geomagnetism/VIO Fused Indoor Localization
Authors: Yang Chaoyong , Cheng Zhenhao , Jia Xiaoxue , Zhang Letian , Li Linyang , Zhao Dongqing
Published: January 2023

For positioning tasks of mobile robots in indoor environments, the emerging positioning technique based on visual inertial odometry (VIO) is heavily influenced by light and suffers from cumulative errors, which cannot meet the requirements of long-term navigation and positioning. In contrast, positioning techniques that rely on indoor signal sources such as 5G and geomagnetism can provide drift-free global positioning results, but their overall positioning accuracy is low. In order to obtain higher precision and more reliable positioning, this paper proposes a fused 5G/geomagnetism/VIO indoor localization method. Firstly, the error back propagation neural network (BPNN) model is used to fuse 5G and geomagnetic signals to obtain more reliable global positioning results; secondly, the conversion relationship from VIO local positioning results to the global coordinate system is established through the least squares principle; and finally, a fused 5G/geomagnetism/VIO localization system based on the error state extended Kalman filter (ES-EKF) is constructed. The experimental results show that the 5G/geomagnetism fusion localization method overcomes the problem of low accuracy of single sensor localization and can provide more accurate global localization results. Additionally, after fusing the local and global positioning results, the average positioning error of the mobile robot in the two scenarios is 0.61 m and 0.72 m. Compared with the VINS-mono algorithm, our approach improves the average positioning accuracy in indoor environments by 69.0% and 67.2%, respectively.
Modeling a Reverse Logistics Supply Chain for End-of-Life Vehicle Recycling Risk Management: A Fuzzy Risk Analysis Approach
Authors: Omosa Geoffrey Barongo , Numfor Solange Ayuni , Kosacka-Olejnik Monika
Published: January 2023

The automotive industry is one of the largest consumers of natural resources, and End-of-Life Vehicles (ELVs) form bulky wastes when they reach the end of their useful life, hence environmental concerns. Efficiency in recycling ELVs is therefore becoming a major concern to address the number of ELVs collected and recycled to minimize environmental impacts. This paper seeks to describe several activities of a closed-loop reverse logistics supply chain for the collection and recycling of ELVs and to identify the related potential risks involved. This study further investigated the potential risks for managing the efficient recycling of ELVs by modeling and viewing the end-of-life vehicle (ELV) recycling system as a reverse logistics supply chain. ELV recycling steps and processes, including collection and transportation, as well as the laws and technologies, were analyzed for risk factor identification and analysis. The major aim of this research is to perform a unified hierarchical risk analysis to estimate the degree of risk preference to efficiently manage the ELV supply chain. This study also proposes a risk assessment procedure using fuzzy knowledge representation theory to support ELV risk analysis. As a result, the identified key risks were ranked in terms of their preference for occurrence in a reverse supply chain of ELV products and mapped into five risk zones, Very Low, Low, Medium-Low, Moderate, Serious, and Critical, for ease of visualization. Hence, with a step-by-step implementation of the presented solution, ELV recycling organizations will see benefits in terms of an improvement in their activities and thus reduced costs that may occur due to uncertainties in their overall ELV business.
Sharing-Economy Ecosystem: A Comprehensive Review and Future Research Directions
Authors: Abdalla Samar , Amankwah-Amoah Joseph , Badewi Amgad
Published: January 2023

This research study reviews the literature on the participants in the sharing economy (SE) ecosystem and its impact on the participants, creating and capturing value through increasing the understanding of the ecosystem’s novel models. The review classifies the participants in the SE ecosystem into primary and secondary participants. The classification is based on the connection to the core network/ecosystem and the role of the participants in the ecosystem. The primary participants are subdivided into groups: customers are subdivided into New Customers (NC) and Current Customers (CC); providers into Product Providers (PP) and Service Providers (SP); and mediators are subdivided into Small and Medium Mediators (SMM) and Large Mediators (LM). The secondary participants are governments sub-grouped into Local Governments (NG) and National Governments (NG); Theories and methodologies within the academic literature on the sharing economy ecosystem are also examined. The study also analyses the influence of digital sharing and explores the value of digital technologies in management strategies and the value of the integration between participants of SE businesses. Recommended future research directions are outlined according to the conducted review.