bluuebunny/crossref_metadata_2025 huggingface dataset:
Adapting a Cancer Screening Education Program for Native American Women with Disabilities
Authors: Armin Julie S. , Williamson Heather J. , Begay Andria , Etcitty Jennifer , Attakai Agnes , Russell Kim , Baldwin Julie A.
Published: August 2022

Like other minoritized groups, people with disabilities experience lack of access to health care. People with intellectual and developmental disabilities (IDD), which are lifelong disabilities diagnosed in childhood requiring varying levels of support for completing daily activities, are less likely to receive preventive health care such as cancer screening. Furthermore, Native American women are less likely than White women to receive cancer screenings. In this qualitative research with Native American women with IDD, their caregivers, healthcare and service providers, and community leaders, we asked, “What are the influences on breast and cervical cancer screening for Native American women with IDD?” with the goal of adapting an existing cancer screening education program. Semi-structured in-depth interviews (N = 48) were audio recorded and transcribed verbatim for analysis. Two coders used a constant comparative method to code and revise the a priori codebook with subthemes and new codes. Results highlighted individual, interpersonal, and community/institutional influences on screening, emphasizing the individual effects of social inequity on this population, the importance of ableist bias in recommending cancer screenings, and opportunities to integrate traditional ways of knowing with allopathic medicine. Results of this work were used to adapt a cancer screening education program for Native American women with IDD.
COVID-19 School vs. Community-Based Outbreak Trends among New Jersey K–12 Schools during the 2020–2021 School Year
Authors: Aggarwal Juhi , Gichura Maureen W. , Campbell Maryanne L. F. , Nguyen Kimberly T. , Shendell Derek G.
Published: August 2022

Identifying potential rapid methods to track COVID-19 trends within schools has become a necessity in understanding how to provide both education and maintain health and safety during a pandemic. This study examined COVID-19 trends and sociodemographic information in New Jersey (NJ) schools during the 2020–2021 school year. A database was compiled for this study in Microsoft Excel using various state and federal resources. Data used in the study are a combination of extracted data from weekly NJ Spotlight reports, weekly NJ COVID-19 Activity Level Index (NJ CALI) reports, and reports of school-based outbreaks via the NJ Department of Health (NJDOH). In 2020–2021, in NJ K–12 schools, the NJDOH defined a school-based outbreak incidence as two or more students and/or adult staff with a laboratory-confirmed positive molecular test for COVID-19 based on transmission occurring on campus. Data were organized into six regions across 21 counties within NJ (3–4 counties per region per NJDOH). COVID-19 trends in NJ schools mirrored trends in their districts, i.e., communities, within the state’s region; noticeably, there were consistently high trends during the winter holiday season (November 2020–January 2021). The cumulative number of incidences of school-based outbreaks remained relatively low but, nevertheless, increased throughout the 2020–2021 school year. This study recommends increased accessibility to COVID-19 reports for school and public health officials, and in the future for data to be reported to identify rates of transmission of other communicable diseases within K–12 students, and to further reinforce established mandates and other preventative measures in public while traveling during holiday seasons.
The Relationship between Physical Activity and College Students’ Mobile Phone Addiction: The Chain-Based Mediating Role of Psychological Capital and Social Adaptation
Authors: Chen Hanwen , Wang Caixia , Lu Tianci , Tao Baole , Gao Yuan , Yan Jun
Published: August 2022

The purpose of this study was to investigate the effects and mechanisms of physical activity on mobile phone addiction among college students. A total of 9406 students, ranging from freshmen to seniors, from 35 colleges in four regions of Jiangsu Province were selected using the whole group sampling method. Questionnaires, particularly the International Physical Activity Questionnaire—Long Form (IPAQ), the positive psychological capital scale (PPQ), the social adjustment diagnostic questionnaire (SAFS), and the mobile phone addiction index scale (MPAI), were administered. We found that physical activity negatively predicted mobile phone addiction among university students. Social adaptation partially mediates between physical activity and mobile phone addiction among university students, with separate mediation of psychological capital playing no indirect role. Psychological capital and social adjustment mediate the chain between physical activity and mobile phone dependence among college students. Our findings suggest that physical activity is an important external factor influencing college students’ mobile phone dependence, and it indirectly affects university students’ mobile phone addiction through psychological capital and social adaptation. Improving the physical activity level of college students, enhancing their psychological capital, and promoting improved social adaptation are important ways to prevent mobile phone addiction among college students.
Application of Advanced Oxidation Technology in Sludge Conditioning and Dewatering: A Critical Review
Authors: Xia Jiahua , Ji Juan , Hu Zhiqiang , Rao Ting , Liu Ankang , Ma Jingqian , Sun Yongjun
Published: August 2022

Sludge dewatering is an important link in sludge treatment. In practical engineering, the dewatering effect of unconditioned sludge is very poor. The use of advanced oxidation technology can improve sludge dewatering performance, reduce sludge capacity, and remove micro-pollutants, which is beneficial for sludge post-treatment and disposal. Based on the current status of sludge conditioning and dehydration, the characteristics of the advanced oxidation method for sludge dehydration were systematically explained using various free radical reaction mechanisms and dehydration conditions. The effects of various advanced oxidation technologies on sludge conditioning and dewatering has been extensively discussed. Finally, the application prospects of the advanced oxidation technology in sludge conditioning and dewatering are presented.
Emotional Intelligence in Ultra-Marathon Runners: Implications for Recovery Strategy and Stress Responses during an Ultra-Endurance Race
Authors: Nicolas Michel , Gaudino Marvin , Bagneux Virginie , Millet Gregoire , Laborde Sylvain , Martinent Guillaume
Published: August 2022

The aim of this research was to investigate the role of trait emotional intelligence (EI) in recovery stress states in a mountain ultra-marathon (MUM) race. Recovery stress states of 13 finishers were assessed before, during, and immediately after the end of an extreme MUM, whereas emotional intelligence was assessed 2 days before the MUM race. Temporal evolutions of recovery stress states were examined. Stress states increased after the race whereas recovery states decreased in all participants. In addition, recovery states were influenced by the trait EI level assessed before the competition. Results supported the hypothesis that trait EI tends to have a positive effect by boosting recovery strategies. In this perspective, trait EI could have a protective role against stress and improve pre-competition mental preparation. High scores of trait EI (in comparison to low scores of trait EI) could have helped athletes to increase recovery states in order to improve their psychological adaptation to one of the most difficult races in the world.
Age-Friendly Communities and Older Adults’ Health in the United States
Authors: Kim Kyeongmo , Buckley Thomas D. , Burnette Denise , Huang Jin , Kim Seon
Published: August 2022

As age-friendly community (AFC) initiatives grow, it will be essential to determine whether older adults who live in an AFC have better health than those who live in other environments. This study uses data from the 2017 AARP AFC Surveys and the AARP Livability Index to assess whether AFCs promote the health of older adults. We analyze data for 3027 adults aged 65 and older who reside in 262 zip code areas. Following AARP guidelines, we allocated the sample into two groups: an AFC group (livability score of 51+; n = 2364) and a non-AFC (score ≤ 50, n = 663). The outcome variable was self-rated health (M = 3.5; SD = 1.1; range: 1–5). We used an inverse probability weighting approach to evaluate whether older adults who live in an AFC reported better self-rated health than those who live in a non-AFC. Findings showed that older adults who lived in an AFC had better self-rated health than those in a non-AFC (b = 0.08, p = 0.027). Compared to non-Hispanic Whites, Black and Hispanic older adults reported worse self-rated health. Inasmuch as living in an AFC can promote the well-being of older adults, policymakers and practitioners should continue to develop and sustain high-quality, accessible built and social environments.
Understanding Moral Distress among Eldercare Workers: A Scoping Review
Authors: Nikunlaakso Risto , Selander Kirsikka , Weiste Elina , Korkiakangas Eveliina , Paavolainen Maria , Koivisto Tiina , Laitinen Jaana
Published: August 2022

The aging of the population in Western countries will increase the use of social and health services in the future. Employees in eldercare are at risk for experiencing moral distress, which is associated with poor work ability. The causes and consequences of moral distress among eldercare workers remain undiscovered. This scoping review investigates the existing studies of causes and consequences of moral distress among eldercare workers. Additionally, it seeks evidence of interventions designed to mitigate moral distress in eldercare workers. Fourteen studies were included in the final review. Most of the included studies were qualitative, aiming to increase understanding of morally challenging situations in eldercare. We also found quantitative studies with cross-sectional designs and small sample sizes. Thus, no reliable evidence of causal effects between moral distress and worker wellbeing in eldercare was found. We found no interventions undertaken to resolve moral distress among eldercare workers, either. More research is needed on the causes and consequences of moral distress and on interventions to mitigate moral distress among eldercare workers. This is of utmost importance to increase the attractiveness of eldercare as a workplace and to improve eldercare workers’ ability to work and sustain long working careers.
Contact Tracing Research: A Literature Review Based on Scientific Collaboration Network
Authors: Li Hui , Zhu Yifei , Niu Yi
Published: August 2022

Contact tracing is a monitoring process including contact identification, listing, and follow-up, which is a key to slowing down pandemics of infectious diseases, such as COVID-19. In this study, we use the scientific collaboration network technique to explore the evolving history and scientific collaboration patterns of contact tracing. It is observed that the number of articles on the subject remained at a low level before 2020, probably because the practical significance of the contact tracing model was not widely accepted by the academic community. The COVID-19 pandemic has brought an unprecedented research boom to contact tracing, as evidenced by the explosion of the literature after 2020. Tuberculosis, HIV, and other sexually transmitted diseases were common types of diseases studied in contact tracing before 2020. In contrast, research on contact tracing regarding COVID-19 occupies a significantly large proportion after 2000. It is also found from the collaboration networks that academic teams in the field tend to conduct independent research, rather than cross-team collaboration, which is not conducive to knowledge dissemination and information flow.
The Coupling Coordination Evolutionary Analysis of Tourism-Ecological Environment-Public Service for the Yellow River Basin of China
Authors: Huang Zhenhui , Wei Wei , Han Ying , Ding Shuangying , Tang Ke
Published: August 2022

In the context of the global pandemic, the development of tourism in the Yellow River Basin is constrained by the dual mechanisms of the decline in the quality of public service and the deterioration of the ecological environment. In order to promote the high-quality development of the ecological environment in the Yellow River Basin, this paper studies the coordinated development of tourism, the ecological environment and public service in the Yellow River Basin by treating tourism, the ecological environment and public service as a whole. Based on the coupling coordination function GM (1,1) grey prediction method and PVAR model, we discuss the characteristics of spatio-temporal differences, evolutionary trends and the interaction mechanism of the coupling coordination degree (CCD) of tourism-ecological environment-public service in nine provinces along the Yellow River Basin in China from 2008 to 2019. The results show that tourism and public service in the Yellow River Basin are closely related, and the protection of the ecological environment and tourism development are not contradictory. In terms of time, the overall trend is stable and upward from the perspective of the CCD of the three systems; in terms of space, Henan, Shandong, and Sichuan provinces have a relatively high level of CCD. While Qinghai, Gansu, Ningxia, Inner Mongolia, Shaanxi and Shanxi provinces have a lower level of CCD, which shows an upward trend from upstream to downstream in the space. The evolutionary trend of the CCD of the three systems in the basin will be upward in all provinces except for the Shandong province in the next five years. Tourism can promote both the ecological environment and public service from the perspective of the mutual influence mechanism.
Response to the Regulation of Video Games under the Youth Media Protection Act: A Public Health Perspective
Authors: Lischer Suzanne , Jeannot Emilien , Brülisauer Lukas , Weber Niels , Khazaal Yasser , Bendahan Samuel , Simon Olivier
Published: August 2022

The Swiss Youth and Media Act, which is about to enter into force, is an attempt to provide a legislative framework for video game use. Among other inclusions, the law intends to make providers more accountable by taking measures to protect minors from harm that can be caused by improper use of video games. However, it is a challenge to create a legal framework that can adequately regulate the evolving features of video games. Legislators must find a suitable regulatory approach which takes into account the fact that there is an increasing convergence between video games and gambling, particularly with the introduction of loot boxes. Moreover, there is a need for regulation, including the prohibition of misleading designs, the introduction of additional protection for minors, and the assurance of transparency of transactions. Appropriate policy legislation and consumer-protection measures are needed to protect people using these types of products, particularly children and adolescents. Further work should focus on assessing game characteristics to refine regulatory models to promote safe gaming. Based on experiences from the field of psychoactive substances as well as that of gambling, it is now a matter of developing a matrix of harm with elaborated categories: a tool that makes it possible to evaluate the potential harms of certain game design in an evidence-based manner.
Effects of Meteorological Factors and Air Pollutants on COVID-19 Transmission under the Action of Control Measures
Authors: Han Fei , Zheng Xinqi , Wang Peipei , Liu Dongya , Zheng Minrui
Published: August 2022

At present, COVID-19 is still spreading, and its transmission patterns and the main factors that affect transmission behavior still need to be thoroughly explored. To this end, this study collected the cumulative confirmed cases of COVID-19 in China by 8 April 2020. Firstly, the spatial characteristics of the COVID-19 transmission were investigated by the spatial autocorrelation method. Then, the factors affecting the COVID-19 incidence rates were analyzed by the generalized linear mixed effect model (GLMMs) and geographically weighted regression model (GWR). Finally, the geological detector (GeoDetector) was introduced to explore the influence of interactive effects between factors on the COVID-19 incidence rates. The results showed that: (1) COVID-19 had obvious spatial aggregation. (2) The control measures had the largest impact on the COVID-19 incidence rates, which can explain the difference of 34.2% in the COVID-19 incidence rates, while meteorological factors and pollutant factors can only explain the difference of 1% in the COVID-19 incidence rates. It explains that some of the literature overestimates the impact of meteorological factors on the spread of the epidemic. (3) The influence of meteorological factors was stronger than that of air pollution factors, and the interactive effects between factors were stronger than their individual effects. The interaction between relative humidity and NO2 was stronger. The results of this study will provide a reference for further prevention and control of COVID-19.
Effectiveness of China’s Protected Areas in Mitigating Human Activity Pressure
Authors: Chen Jian , Shi Hong , Wang Xin , Zhang Yiduo , Zhang Zihan
Published: August 2022

Global protected areas are the key factor in maintaining biodiversity and ecosystem services. However, few studies use human activity pressure to assess the effectiveness of protected areas. This study constructed a human activity pressure index to assess the effectiveness of China’s protected areas, and predicted the change trend in 2050 under the SSP scenarios. The results are as follows: (1) From 2000 to 2020, the pressure of human activities in 75.15% of China’s protected areas is on the rise, accounting for 37.98% of the total area of the reserves. (2) China’s protected areas can relieve the pressure of human activities by 1.37%, and there are regional differences in the effectiveness. (3) Under the SSP scenarios, the protected areas can alleviate the effect of the pressure of the population well. These results can provide a systematic and scientific reference for the planning, construction, evaluation and management of global protected areas.
A Model to Evaluate the Effectiveness of the Maritime Shipping Risk Mitigation System by Entropy-Based Capability Degradation Analysis
Authors: Shen Jun , Ma Xiaoxue , Qiao Weiliang
Published: August 2022

Accurate evaluation of the risk mitigation status of navigating ships is essential for guaranteeing navigational safety. This research mainly focuses on the feasibility and accuracy of evaluating the real effectiveness of a risk mitigation system for navigating ships, including addressing the problem of immeasurableness for risk mitigation capability and determining the degradation regulation of risk mitigation capability over time. The proposed method to solve the problem is an effectiveness evaluation model based on the capability perspective, composed of a capability measurement algorithm based on entropy theory and capability degradation regulation analysis based on numerical process fitting. First, combined with the theoretical framework of a comprehensive defence system, the risk mitigation system designed for navigating ships is reconstructed based on capability building. Second, using a numerical fitting method, the degradation regulation of risk mitigation capability with time is obtained to improve the accuracy of the dynamic evaluation. Finally, referring to entropy theory, the uncertainty of capability is calculated, and then the model is constructed based on this uncertainty to realize the effectiveness evaluation from a capability perspective. The results obtained in an application test of the proposed model indicate that using the entropy of capability can realize an accurate effectiveness evaluation of a risk mitigation system for navigating ships, with a 9% improvement in accuracy, and the Weibull curve fitting is more consistent with capability degradation regulation, with a signification level of 2.5%. The proposed model provides a new path for evaluating the effectiveness of a risk mitigation system for navigating ships from the entropy of capability, and compared with the traditional probabilistic method, the model is more realistic and accurate in actual applications.
Association between Family Dysfunction and Post-Traumatic Stress Disorder in School Students during the Second COVID-19 Epidemic Wave in Peru
Authors: Fernandez-Canani Maria A. , Burga-Cachay Stefany C. , Valladares-Garrido Mario J.
Published: August 2022

Although the effect of the COVID-19 pandemic on children and adolescents’ mental health has been studied, there is still scarce evidence of the influence of nuclear family on the development of post-traumatic stress disorder (PTSD). This study aimed to determine the association between family dysfunction and PTSD in Peruvian high-school students during the COVID-19 pandemic. A cross-sectional study was conducted using a virtual survey administered to 562 high-school students in three schools in Chiclayo, Peru. The dependent variable was PTSD, which was measured with the Child PTSD Symptom Scale. Family dysfunction was the main independent variable, measured with the Family APGAR Questionnaire. Prevalence ratios (PR) and 95% confidence intervals (95% CI) were estimated with generalized linear models. Most of the students were female (88.3%) and the average age was 14.4 years. We found that 21.4% showed severe family dysfunction and 60.3% had PTSD. Students with mild and moderate family dysfunction had 37% (PR: 1.37; 95% CI: 1.14–1.65) and 26% (PR: 1.26; 95% CI: 1.04–1.54) higher PTSD prevalence, respectively. In conclusion, family dysfunction may influence the development of PTSD in adolescents. This study suggests the importance to develop a healthy family environment to help adolescents face critical situations experienced during the pandemic.
The Chain Mediating Effect of Negative Perfectionism on Procrastination: An Ego Depletion Perspective
Authors: Zhang Yan , Bai Xinwen , Yang Wanyi
Published: August 2022

Extant research has consistently demonstrated that negative perfectionism is an important driver of procrastination. However, existing studies suffer from several salient limitations, such as an inadequacy in understanding its mediating mechanism, and the lack of an overarching theoretical framework. Accordingly, the present study adopts the ego depletion theory to investigate how and under what conditions negative perfectionism leads to procrastination. Specifically, we propose that fear of failure and ego depletion serially mediate the effect of negative perfectionism on procrastination, and that cognitive flexibility moderates this chain mediating effect. A three-wave survey consisting of 490 participants, in which negative perfectionism was measured in the first survey, fear of failure and ego depletion were measured in the second, and procrastination was measured in the last survey, lends support to all of our hypotheses. Specifically, our results indicate that (a) negative perfectionism influences procrastination through the chain mediating effect of fear of failure and ego depletion; (b) cognitive flexibility moderates the effect of fear of failure on ego depletion in that the effect is weaker when cognitive flexibility is high rather than low; and (c) cognitive flexibility moderates the chain mediating effect of negative perfectionism on procrastination, such that this chain mediation is weaker when cognitive flexibility is high rather than low. Our findings are discussed in terms of the theoretical contribution to reveal the mechanism by which negative perfectionism results in negative effects from the novel perspective of ego depletion.
Changes in Package Sizes of Savoury Snacks through Exploration of Euromonitor and Industry Perspectives
Authors: Ng Hei Man Emily , Xu Jessica , Liu Qingzhou , Rangan Anna
Published: August 2022

Portion sizes of many energy-dense and nutrient-poor foods and drinks have increased in the past decade, whereas our understanding of the pattern of changes in package sizes remains limited. This study aimed to determine changing trends in sales and package sizes of savoury snacks in Australia, the USA, Japan and Hong Kong, and to investigate industry perspectives for these changes. Sales data (units per capita) between 2006–2020 on savoury snacks were extracted from the Euromonitor International database. Industry perspectives on package size changes were extracted systematically from selected databases, company reports and related websites following the PRISMA-ScR guidelines. The findings showed that sales per capita of savoury snacks of all package sizes increased across all four countries/regions between 2006–2020. Although changes in the proportion of smaller (<100 g) versus larger (>100 g) package size sales in each country/region over time were modest, Japan and Hong Kong exhibited a consistently higher proportion of smaller package sales compared with Australia and the USA (83.3%, 64.4%, 44.3%, 20.2%, respectively). Industry perspectives showed that increasing consumer health consciousness, demands for convenience and portion control were the main contributors to decreasing package sizes of savoury snacks. Industry reports from 2020 showed an increase in larger package size sales due to consumer purchasing behaviour amidst the COVID-19 pandemic.
Innovative In Vitro Strategy for Assessing Aluminum Bioavailability in Oral Care Cosmetics
Authors: Allaria Giorgia , De Negri Atanasio Giulia , Filippini Tommaso , Robino Federica , Dondero Lorenzo , Soggia Francesco , Rispo Francesca , Tardanico Francesca , Ferrando Sara , Aicardi Stefano , Demori Ilaria , Markus Jan , Cortese Katia , Zanotti-Russo Matteo , Grasselli Elena
Published: August 2022

Aluminum is an element found in nature and in cosmetic products. It can interfere with the metabolism of other cations, thus inducing gastrointestinal disorder. In cosmetics, aluminum is used in antiperspirants, lipsticks, and toothpastes. The aim of this work is to investigate aluminum bioavailability after accidental oral ingestion derived from the use of a toothpaste containing a greater amount of aluminum hydroxide than advised by the Scientific Committee on Consumer Safety (SCCS). To simulate in vitro toothpaste accidental ingestion, the INFOGEST model was employed, and the amount of aluminum was measured through the ICP-AES analysis. Tissue barrier integrity was analyzed by measuring transepithelial electric resistance, and the tissue architecture was checked through light microscopy. The margin of safety was also calculated. Overall, our results indicate that the acute exposure to aluminum accidentally ingested from toothpastes is safe for the final user, even in amounts higher than SCCS indications.
Proposing Necessary but Not Sufficient Conditions Analysis as a Complement of Traditional Effect Size Measures with an Illustrative Example
Authors: Greco Ana M. , Guilera Georgina , Maldonado-Murciano Laura , Gómez-Benito Juana , Barrios Maite
Published: August 2022

Even though classic effect size measures (e.g., Pearson’s r, Cohen’s d) are widely applied in social sciences, the threshold used to interpret them is somewhat arbitrary. This study proposes necessary condition analysis (NCA) to complement traditional methods. We explain NCA in light of the current limitations of classical techniques, highlighting the advantages in terms of interpretation and translation into practical terms and recognizing its weaknesses. To do so, we provide an example by testing the link between three independent variables with a relevant outcome in a sample of 235 subjects. The traditional Pearson’s coefficient was obtained, and NCA was used to test if any of the predictors were necessary but not sufficient conditions. Our study also obtains outcome and condition inefficiency as well as NCA bottlenecks. Comparison and interpretation of the traditional and NCA results were made considering recommendations. We suggest that NCA can complement correlation analyses by adding valuable and applicable information, such as if a variable is needed to achieve a certain outcome level and to what degree.
A Teledentistry Pilot Study on Patient-Initiated Care
Authors: Lin Clare , Goncalves Nuno , Scully Ben , Heredia Ruth , Hegde Shalika
Published: August 2022

COVID-19 has challenged the public dental workforce in their ability to continue providing routine oral health care services. To mitigate the risk of COVID-19 transmission to staff and patients, Teledentistry was implemented in many parts of the world, mainly to provide remote consultations, undertake triage, and offer preventive educational sessions. The aim of this paper is to describe Dental Health Services Victoria’s (DHSV) patient-initiated Teledentistry model of care implemented during peak COVID transmission in Victoria. The Teledentistry model supported patient-centered care involving active collaboration and shared decision making between patients, families, and clinicians in designing and managing remote care plans. DHSV’s eligible patient cohort includes disadvantaged population groups with greater oral health needs. Strong emphasis was placed on the simplicity and user friendliness of the Telehealth platform, as well as the support for patients with low technology literacy. Consumers and dental workforce were consulted and modifications to the use of language and services were undertaken before the launch. A total of 2492 patients accessed Telehealth services between May 2020 and April 2021. Approximately 39% of patients were born in a country other than Australia. A total of 489 patient-reported experience measures (PREMs) were received. Patients agreed or strongly agreed that the care they received met their needs (87%); they received answers to their questions (89%); they left their visit knowing what is next (87%); they felt they were taken care of during their visit (90%); and they felt involved in their treatment (89%). Teledentistry enabled patients to initiate access to care and consult with dental workforce remotely and safely during peak pandemic.
Adaptive Geometric Interval Classifier
Authors: Li Shuang , Shan Jie
Published: August 2022

Quantile, equal interval, and natural breaks methods are widely used data classification methods in geospatial analysis and cartography. However, when applied to data with skewed distributions, they can only reveal the variations of either high frequent values or extremes, which often leads to undesired and biased classification results. To handle this problem, Esri provided a compromise method, named geometric interval classification (GIC). Although GIC performs well for various classification tasks, its mathematics and solution process remain unclear. Moreover, GIC is theoretically only applicable to single-peak (single-modal), one-dimensional data. This paper first mathematically formulates GIC as a general optimization problem subject to equality constraint. We then further adapt such formulated GIC to handle multi-peak and multi-dimensional data. Both thematic data and remote sensing images are used in this study. The comparison with other classification methods demonstrates the advantage of GIC being able to highlight both middle and extreme values. As such, it can be regarded as a general data classification approach for thematic mapping and other geospatial applications.
Molecular Basis for Luteolin as a Natural TatD DNase Inhibitor in Trueperella pyogenes
Authors: Zhang Zehui , Guo Yuru , Guo Yueting , Zhang Luyao , Niu Shengli , Tian Chunlian , Han Limei , Zhang Dexian , Liu Mingchun
Published: August 2022

TatD960 and TatD825 are DNases that contribute to biofilm formation and virulence in Trueperella pyogenes (T. pyogenes). Luteolin is a natural flavonoid commonly found in plants that exhibits antimicrobial capacity. Our study aims to investigate the effects of luteolin on TatD DNases as a natural inhibitor. In this research, the expression of tatD genes and TatD proteins in T. pyogenes treated with luteolin was detected, and then the effect of luteolin on the hydrolysis of DNA by TatD DNases was analyzed using agarose gel electrophoresis. Moreover, the interactions between luteolin and TatD DNases were tested using surface plasmon resonance (SPR) assays and molecular docking analysis. After 1/2 MIC luteolin treatment, the transcription of tatD genes and expression of TatD proteins appeared to be reduced in 80–90% of T. pyogenes (n = 20). The gel assay revealed that luteolin can inhibit the activity of TatD DNases. The SPR assay showed that the KD values of luteolin to TatD960 and TatD825 were 6.268 × 10−6 M and 5.654 × 10−6 M, respectively. We found through molecular docking that hydrogen bonding is predominant in the interaction of luteolin and TatD DNases. Our data indicate that luteolin inhibited the ability of TatD DNases by decreasing their binding to DNA. The current study provides an insight into the development of luteolin as a DNase inhibitor in preventing biofilm formation and virulence in T. pyogenes.
An FGFR1-Binding Peptide Modified Liposome for siRNA Delivery in Lung Cancer
Authors: Dong Zhipeng , Yin Yunxue , Luo Jun , Li Bingxia , Lou Fangning , Wang Qiyan , Zhou Qingfa , Ye Baofen , Wang Yue
Published: August 2022

Liposome modification by targeting ligands has been used to mediate specific interactions and drug delivery to target cells. In this study, a new peptide ligand, CP7, was found to be able to effectively bind to FGFR1 through reverse molecular docking and could cooperate with VEGFR3 to achieve targeting of A549 cells. CP7 was modified on the surface of the liposome to construct a targeted and safe nanovehicle for the delivery of a therapeutic gene, Mcl-1 siRNA. Due to the specific binding between CP7 and A549 cells, siRNA-loaded liposome-PEG-CP7 showed increased cellular uptake in vitro, resulting in significant apoptosis of tumor cells through silencing of the Mcl-1 gene, which is associated with apoptosis and angiogenesis. This gene delivery system also showed significantly better antitumor activity in tumor-bearing mice in vivo. All of these suggested that siRNA-loaded liposome-PEG-CP7 could be a promising gene drug delivery system with good bioavailability and minimal side effects for treatment.
Physiological and Biochemical Parameters of Salinity Resistance of Three Durum Wheat Genotypes
Authors: Pastuszak Jakub , Dziurka Michał , Hornyák Marta , Szczerba Anna , Kopeć Przemysław , Płażek Agnieszka
Published: August 2022

The area of farming lands affected by increasing soil salinity is growing significantly worldwide. For this reason, breeding works are conducted to improve the salinity tolerance of important crop species. The goal of the present study was to indicate physiological or biochemical parameters characterizing three durum wheat accessions with various tolerance to salinity. The study was carried out on germinating seeds and mature plants of a Polish SMH87 line, an Australian cultivar ‘Tamaroi’ (salt-sensitive), and the BC5Nax2 line (salt-tolerant) exposed to 0–150 mM NaCl. Germination parameters, electrolyte leakage (EL), and salt susceptibility index were determined in the germinating caryopses, whereas photosynthetic parameters, carbohydrate and phenolic content, antioxidant activity as well as yield were measured in fully developed plants. The parameters that most differentiated the examined accessions in the germination phase were the percentage of germinating seeds (PGS) and germination vigor (Vi). In the fully developed plants, parameters included whether the plants had the maximum efficiency of the water-splitting reaction on the donor side of photosystem II (PSII)–Fv/F0, energy dissipation from PSII–DIo/CSm, and the content of photosynthetic pigments and hydrogen peroxide, which differentiated studied genotypes in terms of salinity tolerance degree. Salinity has a negative impact on grain yield by reducing the number of seeds per spike and the mass of one thousand seeds (MTS), which can be used as the most suitable parameter for determining tolerance to salinity stress. The most salt-tolerant BC5Nax2 line was characterized by the highest PGS, and Vi for NaCl concentration of 100–150 mM, content of chlorophyll a, b, carotenoids, and also MTS at all applied salt concentrations as compared with the other accessions. The most salt-sensitive cv. ‘Tamaroi’ demonstrated higher H2O2 concentration which proves considerable oxidative damage caused by salinity stress. Mentioned parameters can be helpful for breeders in the selection of genotypes the most resistant to this stress.
A Multistage In Silico Study of Natural Potential Inhibitors Targeting SARS-CoV-2 Main Protease
Authors: Elkaeed Eslam B. , Eissa Ibrahim H. , Elkady Hazem , Abdelalim Ahmed , Alqaisi Ahmad M. , Alsfouk Aisha A. , Elwan Alaa , Metwaly Ahmed M.
Published: August 2022

Among a group of 310 natural antiviral natural metabolites, our team identified three compounds as the most potent natural inhibitors against the SARS-CoV-2 main protease (PDB ID: 5R84), Mpro. The identified compounds are sattazolin and caprolactin A and B. A validated multistage in silico study was conducted using several techniques. First, the molecular structures of the selected metabolites were compared with that of GWS, the co-crystallized ligand of Mpro, in a structural similarity study. The aim of this study was to determine the thirty most similar metabolites (10%) that may bind to the Mpro similar to GWS. Then, molecular docking against Mpro and pharmacophore studies led to the choice of five metabolites that exhibited good binding modes against the Mpro and good fit values against the generated pharmacophore model. Among them, three metabolites were chosen according to ADMET studies. The most promising Mpro inhibitor was determined by toxicity and DFT studies to be caprolactin A (292). Finally, molecular dynamics (MD) simulation studies were performed for caprolactin A to confirm the obtained results and understand the thermodynamic characteristics of the binding. It is hoped that the accomplished results could represent a positive step in the battle against COVID-19 through further in vitro and in vivo studies on the selected compounds.
Persistent TLR4 Activation Promotes Hepatocellular Carcinoma Growth through Positive Feedback Regulation by LIN28A/Let-7g miRNA
Authors: Chen I-Ting , Cheng An-Chieh , Liu Yi-Ting , Yan Chieh , Cheng Yi-Chen , Chang Chiung-Fang , Tseng Ping-Hui
Published: August 2022

Chronic inflammation caused by liver damage or infection plays an important role in the development and progression of hepatocellular carcinoma (HCC). The activation of Toll-like receptors 4 (TLR4) is involved in HCC tumorigenesis. Moreover, high TLR4 expression in HCC has been linked to poor prognosis. Although the expression of TLR4 in HCC is relatively low compared to hematopoietic cells, it is important to explore the molecular mechanism leading to the elevation of TLR4 in HCC. In this study, we aimed to investigate the positive regulating loop for TLR4 expression in HCC in response to chronic inflammation. Our results confirm that the mRNA expression of TLR4 and proinflammatory cytokines, including interleukin 6 (IL6) and C-C motif chemokine ligand 2 (CCL2), positively correlate in human HCC samples. High TLR4 expression in HCC is more susceptible to lipopolysaccharide (LPS); TLR4 activation in HCC provides growth and survival advantages and thus promotes tumorigenesis. It has been shown that the LIN28/let-7 microRNA (miRNA) axis is a downstream effector of the TLR4 signal pathway, and let-7 miRNA is a potential post-transcriptional regulator for TLR4. Thus, we investigated the correlation between TLR4 and LIN28A mRNA and let-7g miRNA in HCC clinical samples and found that the expression of TLR4 was positively correlated with LIN28A and negatively correlated with let-7g miRNA. Moreover, by culturing PLC/PRF5 (PLC5) HCC cells in low-dose LPS-containing medium to mimic chronic inflammation for persistent TLR4 activation, the mRNA and protein levels of TLR4 and LIN28A were elevated, and let-7g miRNA was decreased. Furthermore, the 3’ untranslated region (3’UTR) of TLR4 mRNA was shown to be the target of let-7g miRNA, suggesting that inhibition of let-7g miRNA is able to increase TLR4 mRNA. While parental PLC5 cells have a low susceptibility to LPS-induced cell growth, long-term LPS exposure for PLC5 cells leads to increased proliferation, cytokine expression and stemness properties. In conclusion, our studies demonstrate positive feedback regulation for chronic TLR4 activation in the modulation of TLR4 expression level through the LIN28A/let-7g pathway in HCC and suggest a connection between chronic inflammation and TLR4 expression level in HCC for promoting tumorigenesis.
Increased Angiogenesis and Lymphangiogenesis in Adenomyosis Visualized by Multiplex Immunohistochemistry
Authors: Harmsen Marissa J. , Arduç Arda , Bleeker Maaike C. G. , Juffermans Lynda J. M. , Griffioen Arjan W. , Jordanova Ekaterina S. , Huirne Judith A. F.
Published: August 2022

There is evidence for increased angiogenesis in the (ectopic) endometrium of adenomyosis patients under the influence of vascular endothelial growth factor (VEGF). VEGF stimulates both angiogenesis and lymph-angiogenesis. However, information on lymph vessels in the (ectopic) endometrium of adenomyosis patients is lacking. In this retrospective matched case-control study, multiplex immunohistochemistry was performed on thirty-eight paraffin embedded specimens from premenopausal women who had undergone a hysterectomy at the Amsterdam UMC between 2001 and 2018 to investigate the evidence for (lymph) angiogenesis in the (ectopic) endometrium or myometrium of patients with adenomyosis versus controls with unrelated pathologies. Baseline characteristics of both groups were comparable. In the proliferative phase, the blood and lymph vessel densities were, respectively, higher in the ectopic and eutopic endometrium of patients with adenomyosis than in the endometrium of controls. The relative number of blood vessels without α-smooth muscle actinin (α SMA) was higher in the eutopic and ectopic endometrium of adenomyosis patients versus controls. The level of VEGF staining intensity was highest in the myometrium but did not differ between patients with adenomyosis or controls. The results indicate increased angiogenesis and lymphangiogenesis in the (ectopic) endometrium affected by adenomyosis. The clinical relevance of our findings should be confirmed in prospective clinical studies.
Cardiotoxicity of Zebrafish Induced by 6-Benzylaminopurine Exposure and Its Mechanism
Authors: Yang Mengying , Luan Jialu , Xu Yixin , Zhao Chengtian , Sun Mingzhu , Feng Xizeng
Published: August 2022

6-BA is a common plant growth regulator, but its safety has not been conclusive. The heart is one of the most important organs of living organisms, and the cardiogenesis process of zebrafish is similar to that of humans. Therefore, based on wild-type and transgenic zebrafish, we explored the development of zebrafish heart under 6-BA exposure and its mechanism. We found that 6-BA affected larval cardiogenesis, inducing defective expression of key genes for cardiac development (myl7, vmhc, and myh6) and AVC differentiation (bmp4, tbx2b, and notch1b), ultimately leading to weakened cardiac function (heart rate, diastolic speed, systolic speed). Acridine orange staining showed that the degree of apoptosis in zebrafish hearts was significantly increased under 6-BA, and the expression of cell-cycle-related genes was also changed. In addition, HPA axis assays revealed abnormally expressed mRNA levels of genes and significantly increased cortisol contents, which was also consistent with the observed anxiety behavior in zebrafish at 3 dpf. Transcriptional abnormalities of pro- and anti-inflammatory factors in immune signaling pathways were also detected in qPCR experiments. Collectively, we found that 6-BA induced cardiotoxicity in zebrafish, which may be related to altered HPA axis activity and the onset of inflammatory responses under 6-BA treatment.
Cobra Venom Factor Boosts Arteriogenesis in Mice
Authors: Götz Philipp , Azubuike-Osu Sharon O. , Braumandl Anna , Arnholdt Christoph , Kübler Matthias , Richter Lisa , Lasch Manuel , Bobrowski Lisa , Preissner Klaus T. , Deindl Elisabeth
Published: August 2022

Arteriogenesis, the growth of natural bypass blood vessels, can compensate for the loss of arteries caused by vascular occlusive diseases. Accordingly, it is a major goal to identify the drugs promoting this innate immune system-driven process in patients aiming to save their tissues and life. Here, we studied the impact of the Cobra venom factor (CVF), which is a C3-like complement-activating protein that induces depletion of the complement in the circulation in a murine hind limb model of arteriogenesis. Arteriogenesis was induced in C57BL/6J mice by femoral artery ligation (FAL). The administration of a single dose of CVF (12.5 µg) 24 h prior to FAL significantly enhanced the perfusion recovery 7 days after FAL, as shown by Laser Doppler imaging. Immunofluorescence analyses demonstrated an elevated number of proliferating (BrdU+) vascular cells, along with an increased luminal diameter of the grown collateral vessels. Flow cytometric analyses of the blood samples isolated 3 h after FAL revealed an elevated number of neutrophils and platelet-neutrophil aggregates. Giemsa stains displayed augmented mast cell recruitment and activation in the perivascular space of the growing collaterals 8 h after FAL. Seven days after FAL, we found more CD68+/MRC-1+ M2-like polarized pro-arteriogenic macrophages around growing collaterals. These data indicate that a single dose of CVF boosts arteriogenesis by catalyzing the innate immune reactions, relevant for collateral vessel growth.
Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer
Authors: Cacciola Nunzio Antonio , Salzano Angela , D’Onofrio Nunzia , Venneri Tommaso , Cicco Paola De , Vinale Francesco , Petillo Orsolina , Martano Manuela , Maiolino Paola , Neglia Gianluca , Campanile Ciro , Severino Lorella , Merola Carmine , Borrelli Francesca , Balestrieri Maria Luisa , Campanile Giuseppe
Published: August 2022

Recent pharmacological research on milk whey, a byproduct of the dairy industry, has identified several therapeutic properties that could be exploited in modern medicine. In the present study, we investigated the anticancer effects of whey from Mediterranean buffalo (Bubalus bubalis) milk. The antitumour effect of delactosed milk whey (DMW) was evaluated using the HCT116 xenograft mouse model of colorectal cancer (CRC). There were no discernible differences in tumour growth between treated and untreated groups. Nevertheless, haematoxylin and eosin staining of the xenograft tissues showed clearer signs of different cell death in DMW-treated mice compared to vehicle-treated mice. Detailed biochemical and molecular biological analyses revealed that DMW was able to downregulate the protein expression levels of c-myc, phospho-Histone H3 (ser 10) and p-ERK. Moreover, DMW also activated RIPK1, RIPK3, and MLKL axis in tumour tissues from xenograft mice, thus, suggesting a necroptotic effect. The necroptotic pathway was accompanied by activation of the apoptotic pathway as revealed by increased expression of both cleaved caspase-3 and PARP-1. At the molecular level, DMW-induced cell death was also associated with (i) upregulation of SIRT3, SIRT6, and PPAR-γ and (ii) downregulation of LDHA and PPAR-α. Overall, our results unveil the potential of whey as a source of biomolecules of food origin in the clinical setting of novel strategies for the treatment of CRC.