bluuebunny/crossref_metadata_2025 huggingface dataset:
Reaction of Human Monoclonal Antibodies to SARS-CoV-2 Proteins With Tissue Antigens: Implications for Autoimmune Diseases
Authors: Vojdani Aristo , Vojdani Elroy , Kharrazian Datis
Published: January 2021

We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. This extensive immune cross-reactivity between SARS-CoV-2 antibodies and different antigen groups may play a role in the multi-system disease process of COVID-19, influence the severity of the disease, precipitate the onset of autoimmunity in susceptible subgroups, and potentially exacerbate autoimmunity in subjects that have pre-existing autoimmune diseases. Very recently, human monoclonal antibodies were approved for use on patients with COVID-19. The human monoclonal antibodies used in this study are almost identical with these approved antibodies. Thus, our results can establish the potential risk for autoimmunity and multi-system disorders with COVID-19 that may come from cross-reactivity between our own human tissues and this dreaded virus, and thus ensure that the badly-needed vaccines and treatments being developed for it are truly safe to use against this disease.
The Role of Macrophages in Staphylococcus aureus Infection
Authors: Pidwill Grace R. , Gibson Josie F. , Cole Joby , Renshaw Stephen A. , Foster Simon J.
Published: January 2021

Staphylococcus aureusis a member of the human commensal microflora that exists, apparently benignly, at multiple sites on the host. However, as an opportunist pathogen it can also cause a range of serious diseases. This requires an ability to circumvent the innate immune system to establish an infection. Professional phagocytes, primarily macrophages and neutrophils, are key innate immune cells which interact withS. aureus, acting as gatekeepers to contain and resolve infection. Recent studies have highlighted the important roles of macrophages during S. aureus infections, using a wide array of killing mechanisms. In defense, S. aureus has evolved multiple strategies to survive within, manipulate and escape from macrophages, allowing them to not only subvert but also exploit this key element of our immune system. Macrophage-S. aureus interactions are multifaceted and have direct roles in infection outcome. In depth understanding of these host-pathogen interactions may be useful for future therapeutic developments. This review examines macrophage interactions with S. aureusthroughout all stages of infection, with special emphasis on mechanisms that determine infection outcome.
Total Recall: Intestinal TRM Cells in Health and Disease
Authors: Paap Eva-Maria , Müller Tanja M. , Sommer Katrin , Neurath Markus F. , Zundler Sebastian
Published: January 2021

Tissue-resident memory T cells (TRM cells) have crucial functions in host defense in mucosal tissues. They provide local adaptive immune surveillance and allow the fast initiation of targeted adaptive immune responses in case of antigen re-exposure. Recently, an aberrant activation in the case of immunologically mediated diseases has been increasingly acknowledged. As the organ with the largest interface to the environment, the gastrointestinal tract faces billions of antigens every day. Tightly balanced processes are necessary to ensure tolerance towards non-hazardous antigens, but to set up a powerful immune response against potentially dangerous ones. In this complex nexus of immune cells and their mediators, TRM cells play a central role and have been shown to promote both physiological and pathological events. In this review, we will summarize the current knowledge on the homeostatic functions of TRM cells and delineate their implication in infection control in the gut. Moreover, we will outline their commitment in immune dysregulation in gastrointestinal chronic inflammatory conditions and shed light on TRM cells as current and potential future therapeutic targets.
Zebra Mussel Holobionts Fix and Recycle Nitrogen in Lagoon Sediments
Authors: Marzocchi Ugo , Bonaglia Stefano , Zaiko Anastasija , Quero Grazia M. , Vybernaite-Lubiene Irma , Politi Tobia , Samuiloviene Aurelija , Zilius Mindaugas , Bartoli Marco , Cardini Ulisse
Published: January 2021

Bivalves are ubiquitous filter-feeders able to alter ecosystems functions. Their impact on nitrogen (N) cycling is commonly related to their filter-feeding activity, biodeposition, and excretion. A so far understudied impact is linked to the metabolism of the associated microbiome that together with the host constitute the mussel’s holobiont. Here we investigated how colonies of the invasive zebra mussel (Dreissena polymorpha) alter benthic N cycling in the shallow water sediment of the largest European lagoon (the Curonian Lagoon). A set of incubations was conducted to quantify the holobiont’s impact and to quantitatively compare it with the indirect influence of the mussel on sedimentary N transformations. Zebra mussels primarily enhanced the recycling of N to the water column by releasing mineralized algal biomass in the form of ammonium and by stimulating dissimilatory nitrate reduction to ammonium (DNRA). Notably, however, not only denitrification and DNRA, but also dinitrogen (N2) fixation was measured in association with the holobiont. The diazotrophic community of the holobiont diverged substantially from that of the water column, suggesting a unique niche for N2fixation associated with the mussels. At the densities reported in the lagoon, mussel-associated N2fixation may account for a substantial (and so far, overlooked) source of bioavailable N. Our findings contribute to improve our understanding on the ecosystem-level impact of zebra mussel, and potentially, of its ability to adapt to and colonize oligotrophic environments.
TDO2 Promotes the EMT of Hepatocellular Carcinoma Through Kyn-AhR Pathway
Authors: Li Lei , Wang Tao , Li Shanbao , Chen Zhengqian , Wu Junyi , Cao Wanyue , Wo Qi , Qin Xuebin , Xu Junming
Published: January 2021

Tryptophan 2,3-dioxygenase (TDO2), an enzyme involved in tryptophan (Trp) metabolism has been linked with some malignant traits of various cancers. Kyn, the main product of Trp metabolism pathway catalyzed by TDO2 and indoleamine 2,3-dioxygenase (IDO) in tumor cells, was also demonstrated to activate aryl hydrocarbon receptor (AhR), which may regulate cancer growth and invasion in some malignancies. However, whether TDO2 participates in the metastasis and invasion of HCC has not been explored before. The underlying mechanism played by TDO2 in this process still requires further investigation. Here, we demonstrated that overexpression of TDO2 correlates with advanced stage or malignant traits in HCC patients. Knockdown or inhibition of TDO2 suppressed the migration and invasion of HCC cells in vitro and in vivo. Epithelial to mesenchymal transition (EMT) is an essential program happened in the initial phase of cancer metastasis. We found that in HCC cells, TDO2 promoted the EMT process evidenced by altered levels of biomarkers for EMT. Mechanically, TDO2 regulated the Kyn production in HCC cell via activated aryl hydrocarbon receptor (AhR). Together, these results indicate that TDO2 promotes the EMT of hepatocellular carcinoma through activating Kyn-AhR pathway, thereby participating in the metastasis and invasion of HCC.
Sonographic Features of Triple-Negative Breast Carcinomas Are Correlated With mRNA–lncRNA Signatures and Risk of Tumor Recurrence
Authors: Li Jia-wei , Zhou Jin , Shi Zhao-ting , Li Na , Zhou Shi-chong , Chang Cai
Published: January 2021

Background

To determine a correlation between mRNA and lncRNA signatures, sonographic features, and risk of recurrence in triple-negative breast cancers (TNBC).

Methods

We retrospectively reviewed the data from 114 TNBC patients having undergone transcriptome analysis. The risk of tumor recurrence was determined based on the correlation between transcriptome profiles and recurrence-free survival. Ultrasound (US) features were described according to the Breast Imaging Reporting and Data System. Multivariate logistic regression analysis determined the correlation between US features and risk of recurrence. The predictive value of sonographic features in determining tumor recurrence was analyzed using receiver operating characteristic curves.

Results

Three mRNAs (CHRDL1, FCGR1A, and RSAD2) and two lncRNAs (HIF1A-AS2 and AK124454) were correlated with recurrence-free survival in patients with TNBC. Among the three mRNAs, two were upregulated (FCGR1A and RSAD2) and one was downregulated (CHRDL1) in TNBCs. LncRNAs HIF1A-AS2 and AK124454 were upregulated in TNBCs. Based on these signatures, an integrated mRNA–lncRNA model was established using Cox regression analysis to determine the risk of tumor recurrence. Benign-like sonographic features, such as regular shape, circumscribed margin, posterior acoustic enhancement, and no calcifications, were associated with HIF1A-AS2 expression and high risk of tumor recurrence (P<0.05). Malignant-like features, such as irregular shape, uncircumscribed margin, no posterior acoustic enhancement, and calcifications, were correlated with CHRDL1 expression and low risk of tumor recurrence (P<0.05).

Conclusions

Sonographic features and mRNA–lncRNA signatures in TNBCs represent the risk of tumor recurrence. Taken together, US may be a promising technique in determining the prognosis of patients with TNBC.
Only Tumors Angiographically Identified as Hypervascular Exhibit Lower Intraoperative Blood Loss Upon Selective Preoperative Embolization of Spinal Metastases: Systematic Review and Meta-Analysis
Authors: Gong Yining , Wang Changming , Liu Hua , Liu Xiaoguang , Jiang Liang
Published: January 2021

Background

The role of preoperative embolization (PE) in reducing intraoperative blood loss (IBL) during surgical treatment of spinal metastases remains controversial.

Methods

A systematic search was conducted for retrospective studies and randomized controlled trials (RCTs) comparing the IBL between an embolization group (EG) and non-embolization group (NEG) for spinal metastases. IBL data of both groups were synthesized and analyzed for all tumor types, hypervascular tumor types, and non-hypervascular tumor types.

Results

In total, 839 patients in 11 studies (one RCT and 10 retrospective studies) were included in the analysis. For all tumor types, the average IBL did not differ significantly between the EG and NEG in the RCT (P = 0.270), and there was no significant difference between the two groups in the retrospective studies (P = 0.05, standardized mean difference [SMD] = −0.51, 95% confidence interval [CI]: −1.03 to 0.00). For hypervascular tumors determined as such by consensus (n = 542), there was no significant difference between the two groups (P = 0.52, SMD = −0.25, 95% CI: −1.01 to 0.52). For those determined as such using angiographic evidence, the IBL was significantly lower in the EG than in the NEG group, in the RCT (P = 0.041) and in the retrospective studies (P = 0.004, SMD = −0.93, 95% CI: −1.55 to −.30). For IBL of non-hypervascular tumor types, both the retrospective study (P = 0.215) and RCT (P = 0.947) demonstrated no statistically significant differences in IBL between the groups.

Conclusions

Only tumors angiographically identified as hypervascular exhibited lower IBL upon PE in this study. Further exploration of non-invasive methods to identify the vascularity of tumors is warranted.
Detrimental Effects of Chronic L-Arginine Rich Food on Aging Kidney
Authors: Huang Ji , Ladeiras Diogo , Yu Yi , Ming Xiu-Fen , Yang Zhihong
Published: January 2021

The impaired L-arginine/nitric oxide pathway is a well-recognized mechanism for cardiovascular and renal diseases with aging. Therefore, supplementation of L-arginine is widely proposed to boost health or as adjunct therapy for the patients. However, clinical data, show adverse effects and even enhanced mortality in patients receiving long-term L-arginine supplementation. The effects of long-term L-arginine supplementation on kidney aging and the underlying mechanisms remain elusive. Moreover, high protein and high amino acid diet has been thought detrimental for kidney. We therefore investigated effects of chronic dietary L-arginine supplementation on kidney aging. In both young (4 months) and old (18–24 months) mice, animals either receive standard chow containing 0.65% L-arginine or diet supplemented with L-arginine to 2.46% for 16 weeks. Inflammation and fibrosis markers and albuminuria are then analyzed. Age-associated increases intnf-α,il-1β,and il-6,vcam-1,icam-1,mcp1,inos, and macrophage infiltration, collagen expression, and S6K1 activation are observed, which is not favorably affected, but rather further enhanced, by L-arginine supplementation. Importantly, L-arginine supplementation further enhances age-associated albuminuria and mortality particularly in females, accompanied by elevated renal arginase-II (Arg-II) levels. The enhanced albuminuria by L-arginine supplementation in aging is not protected in Arg-II−/−mice. In contrast, L-arginine supplementation increases ROS and decreases nitric oxide production in old mouse aortas, which is reduced in Arg-II−/−mice. The results do not support benefits of long-term L-arginine supplementation. It rather accelerates functional decline of kidney and vasculature in aging. Thus, the long-term dietary L-arginine supplementation should be avoided particularly in elderly population.
Cost-Effectiveness of Point-of-Care A1C Tests in a Primary Care Setting
Authors: Rosa Lorena de Sousa , Mistro Sóstenes , Oliveira Marcio Galvão , Kochergin Clavdia Nickolaevna , Cortes Mateus Lopes , Medeiros Danielle Souto de , Soares Daniela Arruda , Louzado José Andrade , Silva Kelle Oliveira , Bezerra Vanessa Moraes , Amorim Welma Wildes , Barone Mark , Passos Luiz Carlos
Published: January 2021

Objective: We evaluated the cost-effectiveness of the point-of-care A1c (POC-A1c) test device vs. the traditional laboratory dosage in a primary care setting for people living with type 2 diabetes.

Materials and Methods: The Markov model with a 10-year time horizon was based on data from the HealthRise project, in which a group of interventions was implemented to improve diabetes and hypertension control in the primary care network of the urban area of a Brazilian municipality. A POC-A1c device was provided to be used directly in a primary care unit, and for a period of 18 months, 288 patients were included in the point-of-care group, and 1,102 were included in the comparison group. Sensitivity analysis was performed via Monte Carlo simulation and tornado diagram.

Results: The results indicated that the POC-A1c device used in the primary care unit was a cost-effective alternative, which improved access to A1c tests and resulted in an increased rate of early control of blood glucose. In the 10-year period, POC-A1c group presented a mean cost of US$10,503.48 per patient and an effectiveness of 0.35 vs. US$9,992.35 and 0.09 for the traditional laboratory test, respectively. The incremental cost was US$511.13 and the incremental effectiveness was 0.26, resulting in an incremental cost-effectiveness ratio of 1,947.10. In Monte Carlo simulation, costs and effectiveness ranged between $9,663.20–$10,683.53 and 0.33–0.37 for POC-A1c test group, and $9,288.28–$10,413.99 and 0.08–0.10 for traditional laboratory test group, at 2.5 and 97.5 percentiles. The costs for nephropathy, retinopathy, and cardiovascular disease and the probability of being hospitalized due to diabetes presented the greatest impact on the model’s result.

Conclusion: This study showed that using POC-A1c devices in primary care settings is a cost-effective alternative for monitoring glycated hemoglobin A1c as a marker of blood glucose control in people living with type 2 diabetes. According to our model, the use of POC-A1c device in a healthcare unit increased the early control of type 2 diabetes and, consequently, reduced the costs of diabetes-related outcomes, in comparison with a centralized laboratory test.
A Song of Ice and Fire: Cold and Hot Properties of Traditional Chinese Medicines
Authors: Liu Juan , Feng Wuwen , Peng Cheng
Published: January 2021

The theory of cold and hot properties is the basic theory of traditional Chinese medicines (TCMs) and has been successfully applied to combat human diseases for thousands of years. Although the theory of cold and hot is very important to guide the clinical application of TCMs, this ancient theory remains an enigma for a long time. In recent years, more and more researchers have tried to uncover this ancient theory with the help of modern techniques, and the cold and hot properties of a myriad of TCMs have been studied. However, there is no review of cold and hot properties. In this review, we first briefly introduced the basic theories about cold and hot properties, including how to distinguish between the cold and hot properties of TCMs and the classification and treatment of cold and hot syndromes. Then, focusing on the application of cold and hot properties, we take several important TCMs with cold or hot property as examples to summarize their traditional usage, phytochemistry, and pharmacology. In addition, the mechanisms of thermogenesis and antipyretic effect of these important TCMs, which are related to the cold and hot properties, were summarized. At the end of this review, the perspectives on research strategies and research directions of hot and cold properties were also offered.
The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder
Authors: Dong Chun-Liu , Qin Yue , Ma Jin-Xin , Cui Wen-Qiang , Chen Xing-Ru , Hou Li-Ya , Chen Xue-Ying , God’spower Bello-Onaghise , Eliphaz Nsabimana , Qin Jun-Jie , Guo Wen-Xin , Ding Wen-Ya , Li Yan-Hua
Published: January 2021

Plantago asiatica L. is a natural medicinal plant that has been widely used for its various pharmacological effects such as antidiarrheal, anti-inflammatory, and wound healing. This study aims to explore the antidiarrheal active ingredients of Plantago asiatica L. that can be used as quality markers to evaluate P. asiatica L. superfine powder (PSP). Molecular docking experiment was performed to identify the effective components of P. asiatica L., which were further evaluated by an established mouse diarrhea model. Na+/K+-ATPase and creatine kinase (CK) activities and the Na+/K+ concentrations were determined. The gene expression of ckb and Atp1b3 was detected. PSP was prepared and evaluated in terms of the tap density and the angle of repose. The structures of PSPs of different sizes were measured by infrared spectra. The active ingredient contents of PSPs were determined by HPLC. The results indicated that the main antidiarrheal components of P. asiatica L. were luteolin and scutellarein that could increase the concentration of Na+ and K+ by upregulating the activity and gene level of CK and Na+/K+-ATPase. In addition, luteolin and scutellarein could also decrease the volume and weight of small intestinal contents to exert antidiarrheal activity. Moreover, as the PSP size decreased from 6.66 to 3.55 μm, the powder tended to be amorphous and homogenized and of good fluidity, the content of active compounds gradually increased, and the main structure of the molecule remained steady. The optimum particle size of PSP with the highest content of active components was 3.55 μm, and the lowest effective dose for antidiarrhea was 2,000 mg/kg. Therefore, the antidiarrheal active ingredients of PSP were identified as luteolin and scutellarein that exert antidiarrheal activity by binding with Na+/K+-ATPase. PSP was successfully prepared and could be used as a new dosage form for the diarrhea treatment.
Ursolic Acid Regulates Cell Cycle and Proliferation in Colon Adenocarcinoma by Suppressing Cyclin B1
Authors: Yang Minhui , Hu Changxiao , Cao Yibo , Liang Wanling , Yang Xiangdong , Xiao Tianbao
Published: January 2021

Aims: The biological functions of cyclin B1 (CCNB1) in colon adenocarcinoma (COAD) will be explored in this study. Furthermore, the therapeutic effects and potential molecular mechanisms of ursolic acid (UA) in COAD cells will also be investigated in vitro.

Methods: COAD data were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Differentially expressed genes (DEGs) were determined with differential analysis. The biological functions of CCNB1 were analyzed through the GeneCards, the Search Tool for the Retrieval of Interacting Genes (STRING), and the Database for Annotation, Visualization, and Integrated Discovery (DAVID) databases. Therapeutic effects of UA on COAD cell lines HCT-116 and SW-480 were analyzed by CCK-8 and high-content screening (HCS) imaging assay. Flow cytometry was utilized to detect cell cycle changes of SW-480 and HCT-116 cells. Levels of mRNA and expression proteins of HCT-116, SW-480, and normal colon epithelial cells NCM-460 were determined by qRT-PCR and western blot.

Results: CCNB1 was highly expressed and acted as an oncogene in COAD patients. CCNB1 and its interacting genes were significantly enriched in the cell cycle pathway. UA effectively inhibited the proliferation and injured COAD cells. In addition, UA arrested cell cycle of COAD cells in S phase. With regard to the molecular mechanisms of UA, we demonstrated that UA can significantly downregulate CCNB1 and its interacting genes and proteins, including CDK1, CDC20, CCND1, and CCNA2, which contributed to cell cycle blocking and COAD treatment.

Conclusion: Results from this study revealed that UA possesses therapeutic effects on COAD. The anti-COAD activities of UA are tightly related to suppression of CCNB1 and its interacting targets, which is crucial in abnormal cell cycle process.
A Memetic Algorithm for an External Depot Production Routing Problem
Authors: Kayé Bi Kouaï Bertin , Diaby Moustapha , Koivogui Moussa , Oumtanaga Souleymane
Published: January 2021

This study aims to compare the results of a memetic algorithm with those of the two-phase decomposition heuristic on the external depot production routing problem in a supply chain. We have modified the classical scheme of a genetic algorithm by replacing the mutation operator by three local search algorithms. The first local search consists in exchanging two customers visited the same day. The second consists in trying an exchange between two customers visited at consecutive periods and the third consists in removing a customer from his current tour for a better insertion in any tour of the same period. The tests that were carried out on 128 instances of the literature have highlighted the effectiveness of the memetic algorithm developed in this work compared to the two-phase decomposition heuristic. This is reflected by the fact that the results obtained by the memetic algorithm lead to a reduction in the overall average cost of production, inventory, and transport, ranging from 3.65% to 16.73% with an overall rate of 11.07% with regard to the results obtained with the two-phase decomposition heuristic. The outcomes will be beneficial to researchers and supply chain managers in the choice and development of heuristics and metaheuristics for the resolution of production routing problem.
Elucidating the Rhizosphere Associated Bacteria for Environmental Sustainability
Authors: Nwachukwu Blessing Chidinma , Ayangbenro Ayansina Segun , Babalola Olubukola Oluranti
Published: January 2021

The abundance of nutrient accumulation in rhizosphere soils has placed the rhizosphere as an “epicenter” of bacterial concentrations. Nonetheless, over the years, little attention has been given to bacterial inoculants and soil-like substrates. The reason is that many farmers and experiments have focused on chemical fertilizers as an approach to improve plant growth and yield. Therefore, we focused on assessing the application of rhizosphere soil and its associated bacteria for biotechnological applications. This review has been structured into major subunits: rhizosphere soil as a treasure trove for bacterial community concentration, biodegradation of lignocellulose for biofuel production, rhizosphere soil and its bacteria as soil amendments, and the role of rhizosphere soil and its bacteria for bioremediation and biofiltration. Hence, the efficient use of rhizosphere soil and its bacteria in an environmentally friendly way can contribute to healthy and sustainable environments.
Content and Uptake of Ash and Selected Nutrients (K, Ca, S) with Biomass of Miscanthus × giganteus Depending on Nitrogen Fertilization
Authors: Gołąb-Bogacz Izabela , Helios Waldemar , Kotecki Andrzej , Kozak Marcin , Jama-Rodzeńska Anna
Published: January 2021

Fertilisation has a significant impact not only on the yielding, but also on the quality of the harvested biomass. Among energy crops, Miscanthus × giganteus are some of the most important plants used for combustion process. The chemical composition of biomass has significant impact on the quality of combustion biomass. The effect of nitrogen fertilisation (with dose of 60 kg N ha−1) in different terms of biomass sampling on the content and uptake of crude ash, potassium, calcium and sulphur by rhizomes, stems, leaves and the aboveground part of miscanthus was evaluated in the paper. Nitrogen fertilisation contributed to the increase of ash content in the rhizomes and the aboveground part of plants. Independently of nitrogen fertilisation potassium content decreased in the whole vegetation period; in the case of stems this decrease amounted 60%. Calcium content in various parts of plants was highly differentiated compared to potassium content. Average calcium content in the aboveground parts was 2.68 higher compared to rhizomes. Nitrogen fertilisation affected significantly on potassium, calcium and sulphur uptake in all examined parts of plants (except stems in the case of calcium uptake). Uptake of crude ash under nitrogen fertilisation was significantly higher in all examined parts of plants during the whole vegetation period.
Impact of Policy and Factor Intensity on Sustainable Value of European Agriculture: Exploring Trade-Offs of Environmental, Economic and Social Efficiency at the Regional Level
Authors: Czyżewski Bazyli , Guth Marta
Published: January 2021

Although sustainable development is a topic broadly discussed in the literature in relation to existing policy stimulus, a holistic approach to the implementation of sustainability in agriculture—for which there are three dimensions (economic, social and environmental)—is still missing. A regional approach, which averages the entire EU region, could therefore be useful in the long term for recommending directional guidelines for the Common Agricultural Policy (CAP). The objective of this study is to investigate the impact of key groups of CAP instruments and factor intensity on the three above-mentioned aspects of sustainability in the 2004–2017 period, with the assumption that they are all reciprocally related. This goal was achieved by measuring sustainability using the modified sustainable value approach combined with frontier-based nonparametric assessment and applying structural equation modelling, including multilevel random intercept. This research highlights trade-offs between environmental, economic and social efficiency and checks the impact of the EU CAP schemes on the sustainability of environmental, economic, and social dimensions in agriculture. Despite common indications of trade-offs, particularly between economic performance and eco-efficiency, our study shows that in the long term, such feedback has not occurred in any EU regions. Moreover, there are positive interactions between all three dimensions of sustainability from a cross-sectional perspective. The analysis of the impact of CAP subsidies proves that the current system of agri-environmental, set-aside and rural development payments has been effective in the long term, although broader implementation of environmental schemes in regions with lower labour productivity may negatively affect social sustainability.
Grain Yield and Total Protein Content of Organically Grown Oats–Vetch Mixtures Depending on Soil Type and Oats’ Cultivar
Authors: Pużyńska Katarzyna , Pużyński Stanisław , Synowiec Agnieszka , Bocianowski Jan , Lepiarczyk Andrzej
Published: January 2021

The yield and quality of crop mixtures depend on natural and agrotechnical factors and their relationships. This research aimed to analyze the grain yield, its components and total protein content of the organically grown oat–vetch mixture on two different soils and depending on the oat cultivar. The three-year field experiment with two crop rotations was carried out. The experiment was set up in the southern Poland on two soils: Stagnic Luvisol (S.L.) and Haplic Cambisol (H.C.). One of four oat cultivars (‘Celer’, ‘Furman’, ‘Grajcar’ and ‘Kasztan’) was grown with the common vetch cv. ‘Hanka’. The results showed that the grain yield of mixtures was affected mainly by weather conditions. During the dry season, the share of vetch in the grain yield was 46% lower than in the season of regular rainfall. The share of vetch seeds in the mixture’s yield was ca. 21% higher when the mixtures were grown on the S.L. than the H.C. soil. The selection of oats’ cultivar for the mixture with vetch affected significantly the thousand seed mass and protein content in the vetch seeds, 46.2–50.4 g and 270–280 g kg−1, respectively. The mixture with Kasztan cultivar yielded the best and this oat cultivar seemed to be the most appropriate for organic conditions; however, in years with high variability of rainfall distribution its usefulness was less.
Yield and Economic Results of Different Mechanical Pruning Strategies on “Navel Foyos” Oranges in the Mediterranean Area
Authors: Chueca Patricia , Mateu Guillermo , Garcerá Cruz , Fonte Alberto , Ortiz Coral , Torregrosa Antonio
Published: January 2021

Pruning is one of the most manpower-consuming agricultural operations in citrus production. Mechanical pruning can help to reduce pruning time and costs. In order to obtain the knowledge of its effects on the important orange variety “Navel Foyos”, several pruning strategies that include manual pruning and various intensities of mechanical pruning were tested for three years. The results showed that in “Navel Foyos” oranges, the mechanical pruning strategies did not affect the yield nor the size of the fruit in comparison with manual pruning. In conclusion, mechanical strategies are a potential alternative to manual pruning because they reduce the time necessary to prune and the pruning costs, thereby maintaining or even increasing the yield.
Enhancing Drought Tolerance and Striga hermonthica Resistance in Maize Using Newly Derived Inbred Lines from the Wild Maize Relative, Zea diploperennis
Authors: Shaibu Abdulwahab S. , Badu-Apraku Baffour , Ayo-Vaughan Monininuola A.
Published: January 2021

Recurrent drought and Striga hermonthica (Del.) Benth parasitism constrains maize production in sub-Saharan Africa (SSA). Transfer of resistance genes from wild relatives can improve resistance to drought and Striga in tropical maize. The objectives of this study were to (i) determine the combining ability of 12 extra-early yellow maize inbreds derived from Zea diploperennis and tropical maize germplasm; (ii) classify the inbreds into heterotic groups using heterotic grouping based on the general combining ability (GCA) of multiple traits (HGCAMT) method; (iii) examine hybrid performance under contrasting environments; and (iv) examine the stability of hybrid combinations involving the inbreds. Sixty-six diallel crosses involving the inbreds plus four checks were evaluated for two years under drought, Striga-infested and rainfed environments in Nigeria. Significant differences (p < 0.05) were observed for the effects of genotype, environment, genotype × environment, GCA and specific combining ability (SCA) on grain yield and other measured traits. Inbred lines such as TZdEEI 7 × TZEEI 63 derived from Z. diploperennis and tropical germplasm exceeded the checks by a range of 28 to 41%. Across environments, the hybrid TZdEEI 1 × TZdEEI 7, which was derived from Z. diploperennis, was the highest-yielding with a grain yield of 4302 kg ha−1. The results revealed the predominance of GCA over SCA effects for most measured traits, suggesting that additive gene action governed the inheritance of Striga resistance and drought tolerance related traits in the inbreds. The 12 inbreds were classified into three heterotic groups, while TZEEI 79 and TZdEEI 7 were identified as inbred testers and TZdEEI 7 × TZEEI 12 as a single-cross tester across environments. Hybrid TZdEEI 9 × TZEEI 79 was the highest-yielding and most stable. Other promising hybrids were TZdEEI 7 × TZEEI 79, TZdEEI 1 × TZdEEI 7 and TZdEEI 12 × TZEEI 95. These hybrids should be extensively tested on-farm for potential commercialization in SSA. Overall, our results highlighted the importance of harnessing beneficial alleles from wild relatives of maize for improvement of resistance to Striga and tolerance to drought in adapted maize germplasm.
Root Characteristics and Metal Uptake of Maize (Zea mays L.) under Extreme Soil Contamination
Authors: Romdhane Leila , Panozzo Anna , Radhouane Leila , Dal Cortivo Cristian , Barion Giuseppe , Vamerali Teofilo
Published: January 2021

Root characteristics and metal uptake in the maize hybrid Naudi were studied in a pot trial, using soil artificially highly polluted with Zn, Cu, Co, Cd, and Pb. The addition of these metals as sulfates decreased the soil pH and increased electrical conductivity. As a result of increased bioavailability in the soil pore water, significantly higher concentrations of metals, particularly Pb, Cu, and Co, were found in the shoot tissues of maize at the 3–4 leaf stage. While the lowest increase was in Cd (0.89 mg kg−1 vs. 0.33 mg kg−1 for controls), Zn reached a critical threshold of 75 mg kg−1 vs. 2.76 mg kg−1. Fresh and dry weights of shoots and roots, as well as root length, were markedly reduced, whereas root diameter, tip density, and the branching index increased considerably. A significant adaptation strategy by maize in the polluted soil was an increased fraction of coarse root length and a decreased fraction of finer roots. We conclude that maize is very sensitive to multiple metal pollution, suggesting its potential use as a test plant to evaluate contaminated soils. As length was the most affected root characteristic, measurement of this parameter could be a way of screening genotypes for tolerance to metal contamination and possibly salinity. There also is future scope for investigating whether K fertilization might mitigate metal phytotoxicity, in view of the negative correlations between the shoot K concentration and concentrations of the supplied metals.
Identification of Associations between SSR Markers and Quantitative Traits of Maize (Zea mays L.)
Authors: Bocianowski Jan , Nowosad Kamila , Wróbel Barbara , Szulc Piotr
Published: January 2021

Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. Marker-assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. In this paper, the relationships between SSR markers and 26 quantitative traits of hybrid maize varieties (Zea mays L.) were analyzed. Association analyses were performed based on 30 SSR primers in a set of thirteen hybrid maize varieties. A total of 112 SSR markers were detected in these genotypes. The number of alleles per locus ranged from 1 to 17, with the average number of alleles per locus equal to 3.7. The number of molecular markers associated with observed traits ranged from 1 (for the number of kernels in row, ears weight and fresh weight of one plant) to 14 (for damage of maize caused by P. nubilalis) in 2016 as well as from 1 (for soil plant analysis development—SPAD, the number of grains in ear and fresh weight of one plant) to 12 (for carotenoids content) in 2017. The sum of statistically significant associations between SSR markers and at least one trait was equal to one hundred sixty in 2016 as well as one hundred twenty-five in 2017. Marker trait associations (MTAs) were found on the basis of regression analysis. The proportion of the total phenotypic variances of individual traits explained by the marker ranged from 24.4% to 77.7% in the first year of study and from 24.3% to 77.9% in 2017. Twenty-two SSR markers performed a significant effect on at least one tested trait in both years of experiment. The three markers (phi021/4, phi036/3, and phi061/2) can be a good tool in marker-assisted selection because they allow simultaneous selection for multiple traits in both years of study, such as the number of kernels in row and the number of grains in ear (phi021/4), the number of plant after germination, the number of plants before harvest, and the number of ears (phi036/3), as well as moisture of grain and length of ears (phi061/2).
The Effect of Foliar Fertilization on the Resistance of Pea (Pisum sativum L.) Seeds to Mechanical Damage
Authors: Szpunar-Krok Ewa , Kuźniar Piotr , Pawlak Renata , Migut Dagmara
Published: January 2021

The aim of the study was to investigate the effect of foliar fertilization on the morphological and mechanical properties of pea seeds (Pisum sativum L.) and to determine the dependences between the morphological features and chemical composition of pea seeds and their susceptibility to mechanical damage. Field experimentation was carried out in 2015–2017 (south-eastern Poland, 49°59′ N, 21°57′ E). Micronutrient conventional fertilization (N1) and organic fertilizer (N2) were tested on eight pea cultivars. The mechanical parameters assessed included compression force (FD), relative deformation (DR), and energy (ED). Application of foliar fertilizer increased the strength of seeds and their ability to resist mechanical damage under quasi-static loads. The seeds of plants fertilized with N1 were more resistant to mechanical damage than the seeds of plants fertilized with N2. Seeds with larger dimensions, mass, density, and a more spherical shape were damaged under the action of less force and energy, and they deformed less. Seeds with higher protein, fat, ash, and K content and lower NFE, Fe, Zn, and Cu content were more resistant to mechanical damage. The mathematical models created provide knowledge about the impact of foliar fertilizer on the mechanical properties of seeds.
Exogenous Melatonin Improves the Reproductive Outcomes of Yearling Iberian Red Deer (Cervus elaphus hispanicus) Hinds
Authors: Ortiz José Antonio , García-Álvarez Olga , Amo-Salas Mariano , Maroto-Morales Alejandro , Iniesta-Cuerda María , Fernández-Santos María del Rocío , Soler Ana Josefa , Garde José Julián
Published: January 2021

The aim of this study was to assess the effect of melatonin implants on the reproductive performance of yearling Iberian red deer (Cervus elaphus hispanicus) hinds. It also explored exogenous melatonin administration as a tool to minimize the negative effect of a low yearling hind’s liveweight on their reproductive efficiency. In addition, the effect of melatonin-treated yearling hinds on non-treated hinds was studied in order to provide a practical and economical protocol to improve farms’ productivity. A total of 4520 Iberian red deer hinds belonging to the same farm were included in this study. Melatonin (108 mg/hind) implants were administered three-fold every 30 days before the breeding season. Fertility rates, calves’ weights and calving dates were registered for each hind. The results showed that exogenous melatonin increased significantly (p < 0.05) the calves’ weight (32.39 ± 1.07 kg vs. 27.65 ± 1.11 kg for Weight 1calf (July) and 46.59 ± 1.50 kg vs. 41.79 ± 1.54 kg for Weight 2calf (August, at weaning)) and advanced the calving date by 15 days in yearling hinds compared to the non-treated group. In addition, the administration of melatonin implants before the breeding season was able to minimize the negative effect of low yearling hinds’ liveweight (Weight 1hind) on their future reproductive outcomes, as the fertility rates increased by 46% and the calves’ weight increased by 7 kg after the melatonin treatment, regardless of the yearlings’ weight. Finally, when both experimental groups (melatonin and non-treated) were kept separate, higher fertility rates (76.73 ± 7.18% vs. 66.94 ± 7.41%) were observed for the melatonin-treated hinds compared to the non-treated hinds. However, when both groups of yearling hinds were maintained together, no significant differences were observed in their fertility outcomes (78.13 ± 21.26% vs. 78.12 ± 23.32%). Therefore, melatonin implants may be used in yearling Iberian red deer hinds as a management tool to improve their reproductive productivity.
On the Variability of Microbial Populations and Bacterial Metabolites within the Canine Stool. An in-Depth Analysis
Authors: Pinna Carlo , Vecchiato Carla Giuditta , Delsante Costanza , Grandi Monica , Biagi Giacomo
Published: January 2021

Canine faecal microbial populations and metabolome are being increasingly studied to understand the interplay between host and gut microbiome. However, the distribution of bacterial taxa and microbial metabolites throughout the canine stool is understudied and currently no guidelines for the collection, storage and preparation of canine faecal samples have been proposed. Here, we assessed the effects that different sampling points have on the abundance of selected microbial populations and bacterial metabolites within the canine stool. Whole fresh faecal samples were obtained from five healthy adult dogs. Stool subsamples were collected from the surface to the inner part and from three equally sized areas (cranial, central, caudal) along the length axis of the stool log. All samples were finally homogenised and compared before and after homogenisation. Firmicutes, Bacteroidetes, Clostridium cluster I, Lactobacillus spp., Bifidobacterium spp. and Enterococcus spp. populations were analysed, as well as pH, ammonia and short-chain fatty acids (SCFA) concentrations. Compared to the surface of the stool, inner subsamples resulted in greater concentrations of SCFA and ammonia, and lower pH values. qPCR assay of microbial taxa did not show any differences between subsamples. Homogenisation of faeces does not affect the variability of microbial and metabolome data. Although the distribution patterns of bacterial populations and metabolites are still unclear, we found that stool subsampling yielded contradictory result and biases that can affect the final outcome when investigating the canine microbiome. Complete homogenisation of the whole stool is therefore recommended.
Abattoir-Based Measures to Assess Swine Welfare: Analysis of the Methods Adopted in European Slaughterhouses
Authors: De Luca Silvio , Zanardi Emanuela , Alborali Giovanni Loris , Ianieri Adriana , Ghidini Sergio
Published: January 2021

The assessment of swine welfare requires feasible, reliable, and reasonable indicators. On-farm evaluation of pig welfare can provide valuable information to veterinarians and farmers. However, such protocols can result expensive and time-consuming. With this regard, an interest in the appraisal of swine welfare at abattoir has grown over the recent years. In particular, the use of certain lesions collected directly from slaughtered animals to determine the welfare status of pigs has been evaluated by several authors. In the present review, the different methods developed to score lesions collected directly from the body and the viscera of animals slaughtered in European abattoirs (“abattoir-based measures”) are presented. The text specifically focuses on the methods currently available in the literature for the scoring of body, pluck and gastric lesions during post-mortem activities. Moreover, the strengths and weaknesses of abattoir-based measures schemes are discussed. To conclude, the future perspectives of the assessment of pig welfare at the slaughterhouse are described, appealing for a benchmarking system that can be systematically used by veterinarians and other professional figures involved in the process.
Influence of Immunocastration on Slaughter Traits and Boar Taint Compounds in Pigs Originating from Three Different Terminal Sire Lines
Authors: Djurkin Kušec Ivona , Cimerman Emilija , Škrlep Martin , Karolyi Danijel , Gvozdanović Kristina , Komlenić Miodrag , Radišić Žarko , Kušec Goran
Published: January 2021

The aim of the research was to investigate the influence of terminal sire line (TSL) and male category (MC) on carcass and meat quality of commercial fatteners. The study was performed on 180 pigs originating from three terminal sire lines: A (Pietrain × Large White), B (pure Pietrain), and C (Pietrain × Duroc × Large White), being assigned to three groups according to MC: immunocastrates (IC, n = 60), surgical castrates (SC, n = 60) and entire males (EM, n = 60). TSL affected most of the carcass and meat quality traits, together with the androstenone concentration. At the same time, MC had a significant effect on fat thickness, ham circumference, drip loss, cooking loss and androstenone and skatole concentrations. A significant interaction effect was observed for carcass length and ham circumference, as well as for most of the measured meat quality traits (except cooking loss, CIE L*, CIE b*, and Warner Bratzler Shear Force (WBSF)). Among the three investigated sire lines, immunocastration was shown to be most beneficial for fatteners sired by the TSL C. However, if deciding to raise EM, fatteners from the TSL B are recommended in terms of carcass and meat quality, although strategies for avoiding boar taint in their carcasses must be taken into consideration.
Toxic Wasting Disorders in Sheep
Authors: Molín Jéssica , Mendonça Fábio S. , Henderson Eileen E. , Nyaoke Akinyi C. , Ramírez Gustavo A. , Navarro Mauricio A. , Uzal Francisco A. , Asín Javier
Published: January 2021

Infectious and parasitic agents have been frequently associated with debilitating and wasting conditions in sheep. The prevalence of these agents has probably undermined the role of toxic causes as contributors to such disorders. In addition, many of these intoxications frequently produce acute clinical disease with specific and characteristic lesions, thus a causal relationship with the toxic substance may be relatively easy to establish. However, persistent exposure to some of these organic or inorganic toxic substances may lead to emaciation, ill-thrift, and poor external aspect. The anti-nutritional factors and alkaloids of several plants, including pyrrolizidine alkaloids, among others, have also been associated with emaciation and/or poor general performance in sheep flocks. In this review, some of these disorders are discussed with an emphasis on clinical signs and lesions, relevant diagnostic aspects, and available therapeutic approaches. In most cases, demonstrating a history of exposure should be one of the most relevant aspects of the diagnostic approach, and removing the animals from the toxic source is the cornerstone of the majority of the treatment strategies.