bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and application of an in-house library and workflow for gas chromatography–electron ionization–accurate-mass/high-resolution mass spectrometry screening of environmental samples
Authors: Castro Verónica , Quintana José Benito , López-Vázquez Javier , Carro Nieves , Cobas Julio , Bilbao Denis , Cela Rafael , Rodil Rosario
Published: December 2021

This work presents an optimized gas chromatography–electron ionization–high-resolution mass spectrometry (GC-EI-HRMS) screening method. Different method parameters affecting data processing with the Agilent Unknowns Analysis SureMass deconvolution software were optimized in order to achieve the best compromise between false positives and false negatives. To this end, an accurate-mass library of 26 model compounds was created. Then, five replicates of mussel extracts were spiked with a mixture of these 26 compounds at two concentration levels (10 and 100 ng/g dry weight in mussel, 50 and 500 ng/mL in extract) and injected in the GC-EI-HRMS system. The results of these experiments showed that accurate mass tolerance and pure weight factor (combination of reverse-forward library search) are the most critical factors. The validation of the developed method afforded screening detection limits in the 2.5–5 ng range for passive sampler extracts and 1–2 ng/g for mussel sample extracts, and limits of quantification in the 0.6–3.2 ng and 0.1–1.8 ng/g range, for the same type of samples, respectively, for 17 model analytes. Once the method was optimized, an accurate-mass HRMS library, containing retention indexes, with ca. 355 spectra of derivatized and non-derivatized compounds was generated. This library (freely available at https://doi.org/10.5281/zenodo.5647960), together with a modified Agilent Pesticides Library of over 800 compounds, was applied to the screening of passive samplers, both of polydimethylsiloxane and polar chemical integrative samplers (POCIS), and mussel samples collected in Galicia (NW Spain), where a total of 75 chemicals could be identified.
Cauda equina syndrome—a practical guide to definition and classification
Authors: Lavy Chris , Marks Paul , Dangas Katerina , Todd Nicholas
Published: December 2021

Purpose

International uniformity of definition and classification are crucial for diagnosis and management of cauda equina syndrome (CES). They are also useful for clinicians when discussing CES with patients and relatives, and for medicolegal purposes.

Methods

We reviewed published literature using PubMed on definition and classification of cauda equina syndrome since 2000 (21 years). Using the search terms ‘cauda equina’ and ‘definition’ or ‘classification’, we found and reviewed 212 papers.

Results

There were 17 different definitions of CES used in the literature. There were three well-defined methods of classification of CES. The two-stage system of incomplete CES (CESI) versus CES with retention (CESR) is the most commonly used classification, and has prognostic value although the details of this continue to be debated.

Conclusion

We used the existing literature to propose a clear definition of CES. We also drew on peer-reviewed published literature that has helped to amplify and expand the CESI/CESR dichotomy, adding categories that are both less severe than CESI, and more severe than CESR, and we propose clear definitions in a table form to assist current and future discussion and management of CES.
Ordinal Trees and Random Forests: Score-Free Recursive Partitioning and Improved Ensembles
Authors: Tutz Gerhard
Published: December 2021

Existing ordinal trees and random forests typically use scores that are assigned to the ordered categories, which implies that a higher scale level is used. Versions of ordinal trees are proposed that take the scale level seriously and avoid the assignment of artificial scores. The construction principle is based on an investigation of the binary models that are implicitly used in parametric ordinal regression. These building blocks can be fitted by trees and combined in a similar way as in parametric models. The obtained trees use the ordinal scale level only. Since binary trees and random forests are constituent elements of the proposed trees, one can exploit the wide range of binary trees that have already been developed. A further topic is the potentially poor performance of random forests, which seems to have been neglected in the literature. Ensembles that include parametric models are proposed to obtain prediction methods that tend to perform well in a wide range of settings. The performance of the methods is evaluated empirically by using several data sets.
Phospholipid fatty acids in soil—drawbacks and future prospects
Authors: Joergensen Rainer Georg
Published: December 2021

The current opinion and position paper highlights (1) correct assignation of indicator phospholipid fatty acids (PLFA), (2) specificity and recycling of PLFA in microorganisms, and (3) complete extraction and detection of PLFA. The straight-chain PLFA 14:0, 15:0, 16:0, and 17:0 occur in all microorganisms, i.e., also in fungi and not only in bacteria. If the phylum Actinobacteria is excluded from the group of Gram-positive bacteria, all remaining bacteria belong to the bacterial phylum Firmicutes, which should be considered. The PLFA 16:1ω5 should be used as an indicator for the biomass of arbuscular mycorrhizal fungi (AMF) as there is no experimental evidence that they occur in marked amounts in Gram-negative bacteria. Fungal PLFA should embrace the AMF-specific 16:1ω5. In the presence of plants, ergosterol should be used instead of the PLFA 18:2ω6,9 and 18:1ω9 as fungal indicators for Mucoromycotina, Ascomycota, and Basidiomycota. The majority of indicator PLFA are not fully specific for a certain microbial group. This problem might be intensified by recycling processes during decomposition to an unknown extent. Soil handling and extraction conditions should be further optimized. The reliability and accuracy of gas chromatographic separation need to be regularly checked against unintentional variations. PLFA analysis will still be of interest over the next decades as an important independent control of DNA-based methods.
Stenting techniques for coronary bifurcation disease: a systematic review and network meta-analysis demonstrates superiority of double-kissing crush in complex lesions
Authors: Wang Rui , Ding Yaodong , Yang Jiaxin , Wang Kexin , Gao Wen , Fang Zhe , Zhou Yujie , Ge Hailong
Published: December 2021

Objective

This study was aimed to compare different stenting techniques for coronary bifurcation disease (CBD).

Background

Percutaneous coronary intervention (PCI) remains controversial for CBD; over the years, several stent techniques for bifurcation lesions have been used. Current guidelines recommend a provisional single-stent strategy as the preferred method for coronary artery bifurcation lesions. However, several randomized controlled trials (RCT) indicated that two-stent techniques showed better clinical outcomes.

Methods

We systematically searched Embase, PubMed, and Web of Science to include RCTs. The primary endpoint was the major adverse cardiovascular event (MACE). Secondary outcomes were cardiac death, myocardial infarction (MI), target-lesion or target-vessel revascularization (TLR or TVR), and definite or probable stent thrombosis (ST). Finally, we used 26 RCTs and a total of 7257 individuals were randomly assigned to one of the 6 stent techniques and included in this network meta-analysis.

Results

In our network meta-analysis, double-kissing (DK) crush was significantly more superior to other 5 stent techniques in MACEs: OR vs. provisional 0.40 (95% CI 0.28–0.55); vs. culotte 0.40 (95% CI 0.26–0.60). DK crush ranked the most effective treatment for MACE (100%), MI (75%), ST (83%), and TLR (100%) in the rank probabilities analysis. In patients with complex bifurcation lesion defined by DEFINITION criteria, DK crush was notably more efficacious than provisional, culotte, and T-stenting/T-stenting and protrusion (TAP) in MACEs (OR vs. provisional 0.26, 95% CI 0.13–0.52) and TLR (OR vs. provisional 0.24, 95% CI 0.10–0.58).

Conclusion

Compared with other stenting techniques, DK crush had a lower incidence of MACEs in CBD. DK crush was significantly associated with a lower rate of MACEs in patients with complex bifurcation lesions defined by the DEFINITION criterion.

Graphical abstract
Experimental characterisation of porcine subcutaneous adipose tissue under blunt impact up to irreversible deformation
Authors: Lanzl Felicitas , Duddeck Fabian , Willuweit Saskia , Peldschus Steffen
Published: December 2021

A deeper understanding of the mechanical characteristics of adipose tissue under large deformation is important for the analysis of blunt force trauma, as adipose tissue alters the stresses and strains that are transferred to subjacent tissues. Hence, results from drop tower tests of subcutaneous adipose tissue are presented (i) to characterise adipose tissue behaviour up to irreversible deformation, (ii) to relate this to the microstructural configuration, (iii) to quantify this deformation and (iv) to provide an analytical basis for computational modelling of adipose tissue under blunt impact. The drop tower experiments are performed exemplarily on porcine subcutaneous adipose tissue specimens for three different impact velocities and two impactor geometries. An approach based on photogrammetry is used to derive 3D representations of the deformation patterns directly after the impact. Median values for maximum impactor acceleration for tests with a flat cylindrical impactor geometry at impact velocities of 886 mm/s, 1253 mm/s and 2426 mm/s amount to 61.1 g, 121.6 g and 264.2 g, respectively, whereas thickness reduction of the specimens after impact amount to 16.7%, 30.5% and 39.3%, respectively. The according values for tests with a spherically shaped impactor at an impact velocity of 1253 mm/s are 184.2 g and 78.7%. Based on these results, it is hypothesised that, in the initial phase of a blunt impact, adipose tissue behaviour is mainly governed by the behaviour of the lipid inside the adipocytes, whereas for further loading, contribution of the extracellular collagen fibre network becomes more dominant.
Topographical functional correlates of interindividual differences in executive functions in young healthy twins
Authors: Menardi Arianna , Reineberg Andrew E. , Smith Louisa L. , Favaretto Chiara , Vallesi Antonino , Banich Marie T. , Santarnecchi Emiliano
Published: December 2021

Executive functions (EF) are a set of higher-order cognitive abilities that enable goal-directed behavior by controlling lower-level operations. In the brain, those functions have been traditionally associated with activity in the Frontoparietal Network, but recent neuroimaging studies have challenged this view in favor of more widespread cortical involvement. In the present study, we aimed to explore whether the network that serves as critical hubs at rest, which we term network reliance, differentiate individuals as a function of their level of EF. Furthermore, we investigated whether such differences are driven by genetic as compared to environmental factors. For this purpose, resting-state functional magnetic resonance imaging data and the behavioral testing of 453 twins from the Colorado Longitudinal Twins Study were analyzed. Separate indices of EF performance were obtained according to a bifactor unity/diversity model, distinguishing between three independent components representing: Common EF, Shifting-specific and Updating-specific abilities. Through an approach of step-wise in silico network lesioning of the individual functional connectome, we show that interindividual differences in EF are associated with different dependencies on neural networks at rest. Furthermore, these patterns show evidence of mild heritability. Such findings add knowledge to the understanding of brain states at rest and their connection with human behavior, and how they might be shaped by genetic influences.
Evidence for infection in intervertebral disc degeneration: a systematic review
Authors: Granville Smith Isabelle , Danckert Nathan P. , Freidin Maxim B. , Wells Philippa , Marchesi Julian R. , Williams Frances M. K.
Published: December 2021

Purpose

Back pain is a major problem worldwide and is linked to intervertebral disc degeneration and Modic change. Several studies report growth of bacteria following extraction of degenerate discs at spine surgery. A pathophysiological role for infection in back pain has been proposed.

Method

We conducted a PRISMA systematic review. MEDLINE, PubMed, Scopus and Web of Science were searched with the terms Modic change, intervertebral dis*, bacteria, microb*, and infect*. Date limits of 2001–2021 were set. Human studies investigating the role of bacteria in disc degeneration or Modic change in vertebrae were included.

Results

Thirty-six articles from 34 research investigations relating to bacteria in human degenerate discs were found. Cutibacterium acnes was identified in pathological disc material. A ‘candidate bacterium’ approach has been repeatedly adopted which may have biased results to find species a priori, with disc microbial evidence heavily weighted to find C. acnes.

Conclusion

Evidence to date implicates C. acnes identified through culture, microscopy and sequencing, with some suggestion of diverse bacterial colonisation in the disc. This review found studies which used culture methods and conventional PCR for bacterial detection.

Further agnostic investigation using newer methods should be undertaken.
The hot spots conjecture can be false: some numerical examples
Authors: Kleefeld Andreas
Published: December 2021

The hot spots conjecture is only known to be true for special geometries. This paper shows numerically that the hot spots conjecture can fail to be true for easy to construct bounded domains with one hole. The underlying eigenvalue problem for the Laplace equation with Neumann boundary condition is solved with boundary integral equations yielding a non-linear eigenvalue problem. Its discretization via the boundary element collocation method in combination with the algorithm by Beyn yields highly accurate results both for the first non-zero eigenvalue and its corresponding eigenfunction which is due to superconvergence. Additionally, it can be shown numerically that the ratio between the maximal/minimal value inside the domain and its maximal/minimal value on the boundary can be larger than 1 + 10− 3. Finally, numerical examples for easy to construct domains with up to five holes are provided which fail the hot spots conjecture as well.
An application of symplectic integration for general relativistic planetary orbitography subject to non-gravitational forces
Authors: O’Leary Joseph , Barriot Jean-Pierre
Published: December 2021

Spacecraft propagation tools describe the motion of near-Earth objects and interplanetary probes using Newton’s theory of gravity supplemented with the approximate general relativisticn-body Einstein–Infeld–Hoffmann equations of motion. With respect to the general theory of relativity and the long-standing recommendations of the International Astronomical Union for astrometry, celestial mechanics and metrology, we believe modern orbitography software is now reaching its limits in terms of complexity. In this paper, we present the first results of a prototype software titled General Relativistic Accelerometer-based Propagation Environment (GRAPE). We describe the motion of interplanetary probes and spacecraft using extended general relativistic equations of motion which account for non-gravitational forces using end-user supplied accelerometer data or approximate dynamical models. We exploit the unique general relativistic quadratic invariant associated with the orthogonality between four-velocity and acceleration and simulate the perturbed orbits for Molniya, Parker Solar Probe and Mercury Planetary Orbiter-like test particles subject to a radiation-like four-force. The accuracy of the numerical procedure is maintained using a 5-stage,$$10^\mathrm{th}$$10th-order structure-preserving Gauss collocation symplectic integration scheme. GRAPE preserves the norm of the tangent vector to the test particle worldline at the order of$$10^{-32}$$10-32.
Bregman primal–dual first-order method and application to sparse semidefinite programming
Authors: Jiang Xin , Vandenberghe Lieven
Published: December 2021

We present a new variant of the Chambolle–Pock primal–dual algorithm with Bregman distances, analyze its convergence, and apply it to the centering problem in sparse semidefinite programming. The novelty in the method is a line search procedure for selecting suitable step sizes. The line search obviates the need for estimating the norm of the constraint matrix and the strong convexity constant of the Bregman kernel. As an application, we discuss the centering problem in large-scale semidefinite programming with sparse coefficient matrices. The logarithmic barrier function for the cone of positive semidefinite completable sparse matrices is used as the distance-generating kernel. For this distance, the complexity of evaluating the Bregman proximal operator is shown to be roughly proportional to the cost of a sparse Cholesky factorization. This is much cheaper than the standard proximal operator with Euclidean distances, which requires an eigenvalue decomposition.
Reproductive health and endocrine disruption in smallmouth bass (Micropterus dolomieu) from the Lake Erie drainage, Pennsylvania, USA
Authors: Walsh Heather L. , Rafferty Sean D. , Gordon Stephanie E. , Blazer Vicki S.
Published: December 2021

Smallmouth bass Micropterus dolomieu were sampled from three sites within the Lake Erie drainage (Elk Creek, Twentymile Creek, and Misery Bay, an embayment in Presque Isle Bay). Plasma, tissues for histopathological analyses, and liver and testes preserved in RNALater® were sampled from 30 smallmouth bass (of both sexes) at each site. Liver and testes samples were analyzed for transcript abundance with Nanostring nCounter® technology. Evidence of estrogenic endocrine disruption was assessed by the presence and severity of intersex (testicular oocytes; TO) and concentrations of plasma vitellogenin in male fish. Abundance of 17 liver transcripts associated with reproductive function, endocrine activity, and contaminant detoxification pathways and 40 testes transcripts associated with male and female reproductive function, germ cell development, and steroid biosynthesis were also measured. Males with a high rate of TO (87–100%) and plasma vitellogenin were noted at all sites; however, TO severity was greatest at the site with the highest agricultural land cover. Numerous transcripts were differentially regulated among the sites and patterns of transcript abundance were used to better understand potential risk factors for estrogenic endocrine disruption. The results of this study suggest endocrine disruption is prevalent in this region and further research would benefit to identify the types of contaminants that may be associated with the observed biological effects.
Stakeholder consultations on small-scale irrigation schemes’ constraints in Zimbabwe
Authors: Muhoyi Edgar , Mbonigaba Josue
Published: December 2021

Small-scale irrigation schemes (SSIS) have been considered a solution to viability challenges in drought-stricken farming areas in developing countries. However, the schemes face severe constraints. In this paper, relevant constraints are identified and ranked in terms of how serious the limitations are from the perspective of stakeholders in drought-prone areas of the Chipinge District in Zimbabwe. Information for the study was gained through a questionnaire and focus group discussions with small-scale irrigation farmers as well as key informant interviews with government irrigation officials, irrigation managers and members of the local community leadership. The information was garnered between August and December in 2017 with the analysis conducted using descriptive statistics and thematic analysis, guided by the Theory of Constraints and classified in the political, economic, social, technological, environmental and legal (PESTEL) framework. Results indicated that SSIS are important in the drought-prone areas of the Chipinge District regarding food security and income generation. However, technical issues bedevilling the schemes are considered to be the most challenging limitations. The most important constraints—ranked in descending order of gravity—are technical, economic, social, environmental, legal and political challenges. Based on these findings, the research strongly recommends modernising small-scale irrigation schemes’ infrastructure, among other issues, as a priority in Zimbabwe's drought-prone areas.
Particle energization in space plasmas: towards a multi-point, multi-scale plasma observatory
Authors: Retinò Alessandro , Khotyaintsev Yuri , Le Contel Olivier , Marcucci Maria Federica , Plaschke Ferdinand , Vaivads Andris , Angelopoulos Vassilis , Blasi Pasquale , Burch Jim , De Keyser Johan , Dunlop Malcolm , Dai Lei , Eastwood Jonathan , Fu Huishan , Haaland Stein , Hoshino Masahiro , Johlander Andreas , Kepko Larry , Kucharek Harald , Lapenta Gianni , Lavraud Benoit , Malandraki Olga , Matthaeus William , McWilliams Kathryn , Petrukovich Anatoli , Pinçon Jean-Louis , Saito Yoshifumi , Sorriso-Valvo Luca , Vainio Rami , Wimmer-Schweingruber Robert
Published: December 2021

This White Paper outlines the importance of addressing the fundamental science theme “How are charged particles energized in space plasmas” through a future ESA mission. The White Paper presents five compelling science questions related to particle energization by shocks, reconnection, waves and turbulence, jets and their combinations. Answering these questions requires resolving scale coupling, nonlinearity, and nonstationarity, which cannot be done with existing multi-point observations. In situ measurements from a multi-point, multi-scale L-class Plasma Observatory consisting of at least seven spacecraft covering fluid, ion, and electron scales are needed. The Plasma Observatory will enable a paradigm shift in our comprehension of particle energization and space plasma physics in general, with a very important impact on solar and astrophysical plasmas. It will be the next logical step following Cluster, THEMIS, and MMS for the very large and active European space plasmas community. Being one of the cornerstone missions of the future ESA Voyage 2050 science programme, it would further strengthen the European scientific and technical leadership in this important field.
Can Mӧssbauer methods of classifying ordinary chondrites help to identify non-representative samples of these meteorites?
Authors: Jakubowska Martyna , Gałązka-Friedman Jolanta , Woźniak Marek , Bogusz Patrycja , Karwowski Łukasz , Duda Przemysław
Published: December 2021

Mössbauer spectra of nonweathered ordinary chondrites consist of four main mineral phases: olivines, pyroxenes, metallic phase and troilite. These minerals represent more than 95% of the whole mass of an ordinary chondrite. Distribution of these mineral phases in micro-scale is not homogeneous. Nevertheless, preparation of representative sample of ordinary chondrite for Mössbauer measurements is possible. To do that a part of 1 g nonweathered material, selected from inside of meteorite without any specific intention is needed. The Warsaw group has been working on investigation of meteorites for 25 years and has analysed about 150 Mössbauer spectra of various meteorites. Among them we found 15 spectra, which could be suspected of being non-representative. These spectra were obtained from Baszkówka, Amber, Bjurböle, Krasnoi-Ugol and Chelyabinsk meteorites. The analysis of how the samples of meteorites were selected for investigation, has shown that the non-representativeness of samples may be due to: intentional choice of sample, preparation of sample from a too small part of material or the use of non-credible source of meteoritic samples. For confirmation of these assumptions, we used a new method of classification of ordinary chondrites – the 4M method. It turned out that this method is a very useful tool for investigation of non-representative samples of equilibrated ordinary chondrites.
Childhood ADHD and Executive Functioning: Unique Predictions of Early Adolescent Depression
Authors: Fenesy Michelle C. , Lee Steve S.
Published: December 2021

Given the increasing prevalence of adolescent depression, identification of its early predictors and elucidation of the mechanisms underlying its individual differences is imperative. Controlling for baseline executive functioning (EF), we tested separate ADHD dimensions (i.e., inattention, hyperactivity-impulsivity) as independent predictors of early adolescent depression, including temporally-ordered causal mediation by academic functioning and social problems, using structural equation modeling. At baseline, participants consisted of 216 children (67% male) ages 6–9 years old with (n = 112) and without (n = 104) ADHD who subsequently completed Wave 2 and 3 follow-ups approximately two and four years later, respectively. Predictors consisted of separate parent and teacher ratings of childhood ADHD and laboratory-based assessments of key EF domains. At Wave 2, parents and teachers completed normed rating scales of youth academic and social functioning; youth completed standardized assessments of academic achievement. At Wave 3, youth self-reported depression. Baseline inattention positively predicted early adolescent depression whereas childhood hyperactivity-impulsivity and EF did not. Neither academic nor social functioning significantly mediated predictions of depression from baseline ADHD and EF. We consider prediction of early adolescent depression from inattention, including directions for future intervention and prevention research.
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
Authors: da Silva Dias Carolina , Rossi Mariana Correa , Apolonio Emanuel V. P. , dos Santos Rosa Gustavo , Pfeifer João Pedro Hübbe , Hussni Carlos Alberto , Watanabe Marcos Jun , Alves Ana Liz Garcia
Published: December 2021

Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release.
Metformin-loaded β-TCP/CTS/SBA-15 composite scaffolds promote alveolar bone regeneration in a rat model of periodontitis
Authors: Xu Wanghan , Tan Wei , Li Chan , Wu Keke , Zeng Xinyi , Xiao Liwei
Published: December 2021

Periodontitis is a progressive infectious inflammatory disease, which leads to alveolar bone resorption and loss of periodontal attachment. It is imperative for us to develop a therapeutic scaffold to repair the alveolar bone defect of periodontitis. In this study, we designed a new composite scaffold loading metformin (MET) by using the freeze-drying method, which was composed of β-tricalcium phosphate (β-TCP), chitosan (CTS) and the mesoporous silica (SBA-15). The scaffolds were expected to combine the excellent biocompatibility of CTS, the good bioactivity of β-TCP, and the anti-inflammatory properties of MET. The MET-loaded β-TCP/CTS/SBA-15 scaffolds showed improved cell adhesion, appropriate porosity and good biocompatibility in vitro. This MET composite scaffold was implanted in the alveolar bone defects area of rats with periodontitis. After 12 weeks, Micro-CT and histological analysis were performed to evaluate different degrees of healing and mineralization. Results showed that the MET-loaded β-TCP/CTS/SBA-15 scaffolds promoted alveolar bone regeneration in a rat model of periodontitis. To our knowledge, this is the first report that MET-loaded β-TCP/CTS/SBA-15 scaffolds have a positive effect on alveolar bone regeneration in periodontitis. Our findings might provide a new and promising strategy for repairing alveolar bone defects under the condition of periodontitis.
In vitro and in vivo anticancer effect of pH-responsive paclitaxel-loaded niosomes
Authors: Barani Mahmood , Hajinezhad Mohammad Reza , Sargazi Saman , Rahdar Abbas , Shahraki Sheida , Lohrasbi-Nejad Azadeh , Baino Francesco
Published: December 2021

In this study, paclitaxel (PTX)-loaded pH-responsive niosomes modified with ergosterol were developed. This new formulation was characterized in terms of size, morphology, encapsulation efficiency (EE), and in vitro release at pH 5.2 and 7.4. The in vitro efficacy of free PTX and niosome/PTX was assessed using MCF7, Hela, and HUVEC cell lines. In order to evaluate the in vivo efficacy of niosomal PTX in rats as compared to free PTX, the animals were intraperitoneally administered with 2.5 mg/kg and 5 mg/kg niosomal PTX for two weeks. Results showed that the pH-responsive niosomes had a nanometric size, spherical morphology, 77% EE, and pH-responsive release in pH 5.2 and 7.4. Compared with free PTX, we found markedly lower IC50s when cancer cells were treated for 48 h with niosomal PTX, which also showed high efficacy against human cancers derived from cervix and breast tumors. Moreover, niosomal PTX induced evident morphological changes in these cell lines. In vivo administration of free PTX at the dose of 2.5 mg/kg significantly increased serum biochemical parameters and liver lipid peroxidation in rats compared to the control rats. The situation was different when niosomal PTX was administered to the rats: the 5 mg/kg dosage of niosomal PTX significantly increased serum biochemical parameters, but the group treated with the 2.5 mg/kg dose of niosomal PTX showed fewer toxic effects than the group treated with free PTX at the same dosage. Overall, our results provide proof of concept for encapsulating PTX in niosomal formulation to enhance its therapeutic efficacy.
Investigation on tribological behaviors of biodegradable pure Zn and Zn-X (Li, Cu, Ge) binary alloys
Authors: Li Huafang , Huang Jinyan , Zhang Peng , Zhang Qi
Published: December 2021

As a potential biodegradable implant material, zinc (Zn) alloys have attracted increasing attention due to their good biocompatibility and moderate degradation rate. Zn and its alloys are expected to become candidate materials for medical devices. The metals implanted in the human body will inevitably undergo friction in the human body before it is completely degraded. Friction and wear are essential factors which may cause medical devices’ service failure. However, there are still few studies on the friction and wear properties of biodegradable Zn-based alloys in the human body, and most studies just focus on the mechanical properties, degradation properties and biocompatibility of the alloys. Thus, it is crucial to study the friction and wear properties of Zn and its alloys. In the present work, we investigated the tribological properties of biodegradable pure Zn and Zn-X (Li, Cu, Ge) alloys. Our study found that under simulated body fluid and dry friction conditions, the addition of alloying elements Li and Cu can improve the friction properties of Zn. Among the four metals, Zn-0.5Li alloy has the lowest friction coefficient and the best wear resistance. Hank’s solution has lubricating and corrosive effects. That is to say, when the alloy is rubbed in Hank’s solution, it can not only be protected by the lubrication of the solution, but also tribocorrosion will occur as well.
Changes in Subjective Well-Being Over Time: Economic and Social Resources do Matter
Authors: Moro-Egido A. l. , Navarro M. , Sánchez A.
Published: December 2021

This article analyzes the main determinants of changes in subjective well-being over time in Germany distinguishing between long-term and short-term changes. Our findings for the long term indicate that social capital and values and cultural dimensions have the greatest capacity to predict changes in subjective well-being. Likewise, the correlation between economic resources and subjective well-being is weaker due to the small increase registered in household income and because people compare their income with those who are better off and feel envy. In the short term, economic resources have the highest capacity to predict both improvements (ups) and declines (downs) in subjective well-being. Finally, we also suggest that, whenever information is available, personality traits should be taken into account in the analysis of changes in subjective well-being over time in order to achieve more reliable estimates.
The $$\gamma$$ function in quantum theory II. Mathematical challenges and paradoxa
Authors: Mihálka Zs. É. , Nooijen M. , Margócsy Á. , Szabados Á. , Surján P. R.
Published: December 2021

While the square root of Dirac’s $$\delta$$
δ
is not defined in any standard mathematical formalism, postulating its existence with some further assumptions defines a generalized function called $$\gamma (x)$$

γ
(
x
)

which permits a quasi-classical treatment of simple systems like the H atom or the 1D harmonic oscillator for which accurate quantum mechanical energies were previously reported. The so-defined $$\gamma (x)$$

γ
(
x
)

is neither a traditional function nor a distribution, and it remains to be seen that any consistent mathematical approaches can be set up to deal with it rigorously. A straightforward use of $$\gamma (x)$$

γ
(
x
)

generates several paradoxical situations which are collected here. The help of the scientific community is sought to resolve these paradoxa.
Polaron Models with Regular Interactions at Strong Coupling
Authors: Myśliwy Krzysztof , Seiringer Robert
Published: December 2021

We study a class of polaron-type Hamiltonians with sufficiently regular form factor in the interaction term. We investigate the strong-coupling limit of the model, and prove suitable bounds on the ground state energy as a function of the total momentum of the system. These bounds agree with the semiclassical approximation to leading order. The latter corresponds here to the situation when the particle undergoes harmonic motion in a potential well whose frequency is determined by the corresponding Pekar functional. We show that for all such models the effective mass diverges in the strong coupling limit, in all spatial dimensions. Moreover, for the case when the phonon dispersion relation grows at least linearly with momentum, the bounds result in an asymptotic formula for the effective mass quotient, a quantity generalizing the usual notion of the effective mass. This asymptotic form agrees with the semiclassical Landau–Pekar formula and can be regarded as the first rigorous confirmation, in a slightly weaker sense than usually considered, of the validity of the semiclassical formula for the effective mass.
Interpreting Intercepted Communication: A Sui Generis Translational Activity
Authors: Capus Nadja , Havelka Ivana
Published: December 2021

Legal wiretapping has gained importance in law enforcement along with the development of information and communication technology. Understanding the language of intercepted persons is essential for the success of a police investigation. Hence, intercept interpreters, as we suggest calling them in this article, are hired. Little is known about this specific work at the interface between language and law. With this article, we desire to contribute to closing this gap by focussing particularly on the translational activity. Our study identifies a fragmented field of research due to the difficulty in accessing workers in this specific field who interpret in a highly confidential phase of criminal investigations. The findings, which are drawn from scarce studies and our empirical data derived from an online questionnaire for a pilot study in Switzerland, demonstrate the wide range of the performed activity intercept interpreting. This article is the first to present translational activity from the perspective of intercept interpreters. The activity differs in many ways from interpretation in court hearings or police interviews. Hence, we suggest categorising interlingual intercept interpretation as a translational activity sui generis and—since previous research has not done justice to the ethical and deontological questions that intercept interpretation raises—advocate for further transdisciplinary research in this field of translation.
The effect of cytokinins on shoot proliferation, biochemical changes and genetic stability of Rhododendron ‘Kazimierz Odnowiciel’ in the in vitro cultures
Authors: Nowakowska Karolina , Pińkowska Anna , Siedlecka Ewa , Pacholczak Andrzej
Published: December 2021

Shoot proliferation is a very important micropropagation phase, decisive for economic efficiency of this method for a given taxon. To obtain a high multiplication ratio and a good quality of microshoots a detailed propagation protocol must be developed for particular species or even cultivars.Rhododendron‘Kazimierz Odnowiciel’ is a relatively new cultivar distinguished by large, beautiful flowers and high frost resistance so there is a need to develop an efficient method of its propagation to satisfy a growing demand for this plant. The aim of the experiment was to evaluate effects of cytokinins: meta-Topolin (mT), zeatin (ZEA), 6-benzyladenine (BA), thidiazuron (TDZ), 2-isopentenyladenine (2iP), or the combination of 2iP+ZEA on proliferation of shoots inR.‘Kazimierz Odnowiciel’ cultured on Anderson’s medium (AN). Biochemical changes in plant material affected by cytokinins during this phase of micropropagation were determined and occurrence of genetical changes was followed using ISSR markers. TDZ, ZEA or the combination of ZEA+2iP resulted in 100% explant regeneration. On the medium with TDZ or ZEA over two new shootsperexplant were produced but the highest proliferation was attained on the medium containing ZEA+2iP – over three shootsperexplant. Microshoots developed in this treatment had also the highest contents of chlorophyll, carotenoids and soluble sugars as well as the highest catalase activity. Microshoots formed on the medium with zeatin showed the lowest polymorphism (below 4%) relative to a stock plant.
Environmental footprint of regular and intensive inpatient care in a large US hospital
Authors: Prasad Purnima Aishwarya , Joshi Dhruvi , Lighter Jennifer , Agins Jenna , Allen Robin , Collins Michael , Pena Foohel , Velletri Joan , Thiel Cassandra
Published: December 2021

Purpose

Environmental sustainability is a growing concern to healthcare providers, given the health impacts of climate change and air pollution, and the sizable footprint of healthcare delivery itself. Though many studies have focused on environmental footprints of operating rooms, few have quantified emissions from inpatient stays. This study quantifies solid waste and greenhouse gas emissions (GHGs) per bed-day in a regular inpatient (low intensity) and intensive care unit (high intensity).

Methods

This study uses hybrid environmental life cycle assessment (LCA) to quantify average emissions associated with resource use in an acute inpatient unit with 49 beds and 14,427 hospitalization days and an intensive care unit (ICU) with 12 beds and 2536 hospitalization days. The units are located in a single tertiary, private hospital in Brooklyn, NY, USA.

Results and discussion

An acute care unit generates 5.5 kg of solid waste and 45 kg CO2-e per hospitalization day. The ICU generates 7.1 kg of solid waste and 138 kg CO2-e per bed day. Most emissions originate from purchase of consumable goods, building energy consumption, purchase of capital equipment, food services, and staff travel.

Conclusions

The ICU generates more solid waste and GHGs per bed day than the acute care unit. With resource use and emission data, sustainability strategies can be effectively targeted and tested. Medical device and supply manufacturers should also aim to minimize direct solid waste generation.
Variable and constraint reduction techniques for the temporal bin packing problem with fire-ups
Authors: Martinovic John , Strasdat Nico , Valério de Carvalho José , Furini Fabio
Published: December 2021

The aim of this letter is to design and computationally test several improvements for the compact integer linear programming (ILP) formulations of the temporal bin packing problem with fire-ups (TBPP-FU). This problem is a challenging generalization of the classical bin packing problem in which the items, interpreted as jobs of given weight, are active only during an associated time window. The TBPP-FU objective function asks for the minimization of the weighted sum of the number of bins, viewed as servers of given capacity, to execute all the jobs and the total number of fire-ups. The fire-ups count the number of times the servers are activated due to the presence of assigned active jobs. Our contributions are effective procedures to reduce the number of variables and constraints of the ILP formulations proposed in the literature as well as the introduction of new valid inequalities. By extensive computational tests we show that substantial improvements can be achieved and several instances from the literature can be solved to proven optimality for the first time.
Epigenetic modification associated with climate regulates betulin biosynthesis in birch
Authors: Wang Jiang , Chen Bowei , Ali Shahid , Zhang Tianxu , Wang Yu , Zhang He , Wang Lishan , Zhang Yonglan , Xie Linan , Jiang Tingbo , Yin Jing , Sederoff Heike W. , Zinta Gaurav , Sederoff Ronald R. , Li Yuhua , Zhang Qingzhu
Published: December 2021

The Betula genus contains pentacyclic triterpenoid betulin known for its environmental adaptation and medicinal properties. However, the mechanisms underlying betulin biosynthesis responding to climate change remain unclear. In this study, the role of epigenetic modification (DNA methylation) in betulin biosynthesis was examined and how climatic factors influence it. Whole-genome bisulfite sequencing was performed for greenhouse-grown Chinese white birch (Betula platyphylla Sukaczev) treated with DNA methylation inhibitor zebularine (ZEB) and a natural birch population in Northeast China. ZEB treatment significantly affected the CHH methylation level of transposable elements and betulin content in a hormesis dose-dependent manner. The methylation and expression of bHLH9, a key transcriptional factor controlling betulin biosynthesis, were also consistently affected by ZEB treatment as a hormetic dose–response. In the natural population, there was a positive correlation between promoter methylation of bHLH9 and summer precipitation, while winter temperature was negatively correlated. Thus climate-dependent methylation of bHLH9 regulates the expression of downstream genes involved in betulin biosynthesis. This study highlights the role of environmental signals to induce epigenetic changes that result in betulin production, possibly helping to develop resilient plants to combat ongoing climate change and enhance secondary metabolite production.
The coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds
Authors: Zhang Min , Zhu Jiaojun
Published: December 2021

Elucidating the regulatory mechanisms of environmental factors on seed dormancy and germination will provide guidance for tree regeneration. Toward understanding the coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds, we set up three light conditions (L200: 200 μmol m−2 s−1, L20: 20 μmol m−2 s−1, L0: 0 μ m−2 s−1) and four storage temperatures [T-5: − 5 °C (50 days), T5: − 5 °C (50 days) + 5 °C (50 days), T25: − 5 °C(50 days) + 5 °C (50 days) + 25 °C (50 days), T15: − 5 °C (50 days) + 5 °C (50 days) + 25 °C (50 days) + 15 °C (50 days)] using imbibed seeds, then quantified phytohormones gibberellic acid (GA3) and abscisic acid (ABA) during the stratification. Germination percentage (GP), mean germination time (TM), and germination value (GV) under 25/15 °C temperature and the three light conditions were then determined. Phytohormone levels and germination performances were significantly affected by light and temperature. No consistent trend was found between the phytohormone levels and GP caused by light levels. Under the three light conditions, ABA concentrations in the embryo and endosperm decreased as storage temperature shifted from T-5 to T25 and increased from T25 to T15; GA3 decreased in nearly all four storage temperatures. GP reached 40–60% in T25 storage without light irradiance. In the three light conditions, GP and GV were higher at T5 and T25 than at T-5 and T15; so T5 and T25 are considered as optimum storage temperatures for dormancy release and germination. At optimum temperatures, light (L200, L20) significantly increased the GP and GV compared with the dark (L0). At L200 and L20, significant negative correlations between GV and the ABA concentrations and positive correlations between GV and GA/ABA in the seed embryo were found. Temperature played a more important role in primary dormancy release and germination; light was unnecessary for primary dormancy release. Light facilitated seed germination at optimum temperatures. The dormancy release and germination of P. koraiensis seeds were controlled by a decrease in ABA concentrations or an increase in GA/ABA induced by temperature variations.
Process design of a novel combination of peel grinding and deep rolling
Authors: Denkena Berend , Kroedel Alexander , Gartzke Tobias
Published: December 2021

Grinding is mostly considered as a finishing operation by which a high surface quality is achieved. An increase in productivity is therefore limited by maintained surface properties such as the roughness or tensile residual stresses. Thus, a roughing operation is inevitable followed by a finishing operation, while both operations are separated, leading to larger cycle times and process costs. In this paper, a novel process combination is investigated in which the roughing is done by grinding and the finishing operation by deep rolling within one tool setup. In this way, both processes are conducted parallel within the primary processing time. The objective of this study is the knowledge of the characteristics of this process combination with regard to the workpiece surface integrity. Therefore, shafts are ground in peel grinding with varying grinding wheel types and process parameters and subsequently machined with deep rolling. The process combination is evaluated with regard to the process forces and the resulting surface properties. In addition, experiments using the process combination were conducted in order to investigate the transferability of the results towards the process combination. By this approach, it was found that the surface roughness was reduced up to 80% by deep rolling showing the potential of the process combination.