bluuebunny/crossref_metadata_2025 huggingface dataset:
Olfactory Preference of Drosophila suzukii Shifts between Fruit and Fermentation Cues over the Season: Effects of Physiological Status
Authors: Clymans Rik , Van Kerckvoorde Vincent , Bangels Eva , Akkermans Wannes , Alhmedi Ammar , De Clercq Patrick , Beliën Tim , Bylemans Dany
Published: July 2019

Worldwide monitoring programs of the invasive fruit pest Drosophila suzukii Matsumura (Diptera: Drosophilidae), using fermentation baits like apple cider vinegar (ACV), revealed a counterintuitive period of low trap catches during summer, followed by an autumn peak. In this study, we demonstrate that ACV baited traps indeed provide a distorted image of the D. suzukii population dynamics as it is possible to capture higher numbers during this “low capture period” with synthetic lures. It was hypothesised that the preference of D. suzukii populations for fermentation cues like ACV is most pronounced during autumn, winter and spring, while the flies prefer fresh fruit cues during summer and that this seasonal preference is related to the changing physiology of the flies over the season. To test this hypothesis, the preference between fermentation cues (ACV) and host fruits (strawberries) and the effect of physiology (sex, seasonal morphology and feeding, mating and reproductive status) was investigated both in olfactometer laboratory experiments and a year-round field preference experiment. In olfactometer experiments we demonstrated that protein deprived females, virgin females with a full complement of unfertilised eggs and males show a strong preference for fermentation cues while fully fed reproductive summer morph females generally prefer fruit cues. These findings indicate that D. suzukii is attracted to fermentation volatiles in search of (protein-rich) food and to fruit volatiles in search of oviposition substrates. Winter morph and starved females displayed indiscriminating olfactory behaviour. In the field preference experiment, the hypothesised seasonal shift between fermentation and fruit cues was confirmed. This shift appeared to be highly temperature-related and was similarly observed for summer and winter morphs.
Modeling Potential Habitat for Amblyomma Tick Species in California
Authors: Pascoe Emily L. , Marcantonio Matteo , Caminade Cyril , Foley Janet E.
Published: July 2019

The Amblyomma genus of ticks comprises species that are aggressive human biters and vectors of pathogens. Numerous species in the genus are undergoing rapid range expansion. Amblyomma ticks have occasionally been introduced into California, but as yet, no established populations have been reported in the state. Because California has high ecological diversity and is a transport hub for potentially parasitized humans and animals, the risk of future Amblyomma establishment may be high. We used ecological niche modeling to predict areas in California suitable for four tick species that pose high risk to humans: Amblyomma americanum, Amblyomma maculatum, Amblyomma cajennense and Amblyomma mixtum. We collected presence data in the Americas for each species from the published literature and online databases. Twenty-three climatic and ecological variables were used in a MaxEnt algorithm to predict the distribution of each species. The minimum temperature of the coldest month was an important predictor for all four species due to high mortality of Amblyomma at low temperatures. Areas in California appear to be ecologically suitable for A. americanum, A. maculatum, and A. cajennense, but not A. mixtum. These findings could inform targeted surveillance prior to an invasion event, to allow mitigation actions to be quickly implemented.
A Building-Block Approach to State-Space Modeling of DC-DC Converter Systems
Authors: Herbst Gernot
Published: July 2019

Small-signal models of DC-DC converters are often based on a state-space averaging approach, from which both control-oriented and other frequency-domain characteristics, such as input or output impedance, can be derived. Updating these models when extending the converter by filters or non-trivial loads, or adding control loops, can become a tedious task, however. To simplify this potentially error-prone process, a modular modeling approach is being proposed in this article. It consists of small state-space models for certain building blocks of a converter system on the one hand, and standardized operations for connecting these subsystem models to an overall converter system model on the other hand. The resulting state-space system model builds upon a two-port converter description and allows the extraction of control-oriented and impedance characteristics at any modeling stage, be it open loop or closed loop, single converter or series connections of converters. The ease of creating more complex models enabled by the proposed approach is also demonstrated with examples comprising multiple control loops or cascaded converters.
Artificial Intelligence-Based Classification of Multiple Gastrointestinal Diseases Using Endoscopy Videos for Clinical Diagnosis
Authors: Owais Muhammad , Arsalan Muhammad , Choi Jiho , Mahmood Tahir , Park Kang Ryoung
Published: July 2019

Various techniques using artificial intelligence (AI) have resulted in a significant contribution to field of medical image and video-based diagnoses, such as radiology, pathology, and endoscopy, including the classification of gastrointestinal (GI) diseases. Most previous studies on the classification of GI diseases use only spatial features, which demonstrate low performance in the classification of multiple GI diseases. Although there are a few previous studies using temporal features based on a three-dimensional convolutional neural network, only a specific part of the GI tract was involved with the limited number of classes. To overcome these problems, we propose a comprehensive AI-based framework for the classification of multiple GI diseases by using endoscopic videos, which can simultaneously extract both spatial and temporal features to achieve better classification performance. Two different residual networks and a long short-term memory model are integrated in a cascaded mode to extract spatial and temporal features, respectively. Experiments were conducted on a combined dataset consisting of one of the largest endoscopic videos with 52,471 frames. The results demonstrate the effectiveness of the proposed classification framework for multi-GI diseases. The experimental results of the proposed model (97.057% area under the curve) demonstrate superior performance over the state-of-the-art methods and indicate its potential for clinical applications.
A Systematic Review of the Clinical Value and Applications of Three-Dimensional Printing in Renal Surgery
Authors: Lupulescu Catalina , Sun Zhonghua
Published: July 2019

The purpose of this systematic review is to collate and analyse the current literature which examines clinical applications of 3D printing for renal disease, alongside cost and time duration factors associated with the printing process. A comprehensive search of the literature was performed across five different databases to identify studies that qualitatively and quantitatively assessed the value of 3D-printed kidney models for renal disease. Twenty-seven studies met the selection criteria for inclusion in the review. Twenty-five were original studies, and two were case reports. Of the 22 studies reporting a qualitative evaluation, the analysis of findings demonstrated the value of the 3D-printed models in areas of clinician and patient education, and pre-surgical simulation for complex cases of renal disease. Of five studies performing a quantitative analysis, the analysis of results displayed a high level of spatial and anatomical accuracy amongst models, with benefits including reducing estimated blood loss and risk of intra-operative complications. Fourteen studies evaluated manufacturing costs and time duration, with costs ranging from USD 1 to 1000 per model, and time duration ranging from 15 min to 9 days. This review shows that the use of customised 3D-printed models is valuable in the education of junior surgeons as well as the enhancement of operative skills for senior surgeons due to a superior visualisation of anatomical networks and pathologic morphology compared to volumetric imaging alone. Furthermore, 3D-printed kidney models may facilitate interdisciplinary communication and decision-making regarding the management of patients undergoing operative treatment for renal disease. It cannot be suggested that a more expensive material constitutes a higher level of user-satisfaction and model accuracy. However, higher costs in the manufacturing of the 3D-printed models reported, on average, a slightly shorter time duration for the 3D-printing process and total manufacturing time.
Predicting the Functional Outcome of Adult Patients with Status Epilepticus
Authors: Lin Chih-Hsiang , Ho Chen-Jui , Lu Yan-Ting , Shih Fu-Yuan , Chuang Yao-Chung , Tsai Meng-Han
Published: July 2019

Patients that survive status epilepticus (SE) may suffer from neurological and cognitive deficits that cause severe disabilities. An effective scoring system for functional outcome prediction may help the clinician in making treatment decisions for SE patients. Three scoring systems, namely the Status Epilepticus Severity Score (STESS), the Epidemiology-Based Mortality Score in Status Epilepticus (EMSE), and the Encephalitis-Nonconvulsive Status Epilepticus-Diazepam Resistance-Image Abnormalities-Tracheal Intubation (END-IT), have been developed in the past decade to predict the outcomes of patients with SE. Our study aimed at evaluating the effectiveness of these scores in predicting the function outcomes both at and after discharge in SE patients. We retrospectively reviewed the clinical data of 55 patients admitted to our neurological intensive care unit between January 2017 and December 2017. The clinical outcomes at discharge and at last follow-up were graded using the modified Rankin Scale. Our research indicated that STESS was the most sensitive and EMSE was the most specific predictive scoring method for SE outcome prediction. On the other hand, END-IT predicted functional outcomes in SE patients poorly. We concluded that STESS and EMSE can accurately predict the functional outcomes in SE patients both at discharge and the follow-up period.
Habitat Climate Change Vulnerability Index Applied to Major Vegetation Types of the Western Interior United States
Authors: Comer Patrick J. , Hak Jon C. , Reid Marion S. , Auer Stephanie L. , Schulz Keith A. , Hamilton Healy H. , Smyth Regan L. , Kling Matthew M.
Published: July 2019

We applied a framework to assess climate change vulnerability of 52 major vegetation types in the Western United States to provide a spatially explicit input to adaptive management decisions. The framework addressed climate exposure and ecosystem resilience; the latter derived from analyses of ecosystem sensitivity and adaptive capacity. Measures of climate change exposure used observed climate change (1981–2014) and then climate projections for the mid-21st century (2040–2069 RCP 4.5). Measures of resilience included (under ecosystem sensitivity) landscape intactness, invasive species, fire regime alteration, and forest insect and disease risk, and (under adaptive capacity), measures for topo-climate variability, diversity within functional species groups, and vulnerability of any keystone species. Outputs are generated per 100 km2 hexagonal area for each type. As of 2014, moderate climate change vulnerability was indicated for >50% of the area of 50 of 52 types. By the mid-21st century, all but 19 types face high or very high vulnerability with >50% of the area scoring in these categories. Measures for resilience explain most components of vulnerability as of 2014, with most targeted vegetation scoring low in adaptive capacity measures and variably for specific sensitivity measures. Elevated climate exposure explains increases in vulnerability between the current and mid-century time periods.
Social Impacts of Land Acquisition for Oil and Gas Development in Uganda
Authors: Ogwang Tom , Vanclay Frank
Published: July 2019

Uganda’s oil and gas sector has transitioned from the exploration phase to the development phase in preparation for oil production (the operations phase). The extraction, processing, and distribution of oil require a great deal of infrastructure, which demands considerable acquisition of land from communities surrounding project sites. Here, we examine the social impacts of project land acquisition associated with oil production in the Albertine Graben region of Uganda. We specifically consider five major oil related projects that have or will displace people, and we discuss the consequences of this actual or future displacement on the lives and livelihoods of local people. The projects are: Tilenga; Kingfisher; the East African Crude Oil Pipeline; the Kabaale Industrial Park; and the Hoima–Kampala Petroleum Products Pipeline. Our findings reveal both positive and negative outcomes for local communities. People with qualifications have benefited or will benefit from the job opportunities arising from the projects and from the much-needed infrastructure (i.e., roads, health centres, airport) that has been or will be built. However, many people have been displaced, causing food insecurity, the disintegration of social and cultural cohesion, and reduced access to social services. The influx of immigrants has increased tensions because of increasing competition for jobs. Crime and social issues such as prostitution have also increased and are expected to increase.
Hot Deformation Behavior and Microstructural Evolution of a Novel β-Solidifying Ti–43Al–3Mn–2Nb–0.1Y Alloy
Authors: Wu Qianqian , Cui Ning , Xiao Xiaohong , Wang Xiaopeng , Zhao Ertuan
Published: July 2019

In this paper, the hot deformability and mechanical properties of a novel Mn- and Nb- containing TiAl alloy were studied systematically with the use of isothermal compression experiments. The results show that the alloy has low deformation resistance and a low activation energy (392 KJ/mol), suggesting that the alloy has good hot deformability. A processing map was established, which shows that the present alloy has a smaller instability region and wider hot working window compared with other TiAl alloys. Microstructural observation shows that the initial lamellae completely transformed into fine equiaxial γ grains when the alloy was compressed at 1200 °C/0.01 s−1, which corresponds to the optimum deformation condition. Based on the above results, an intact TiAl billet was successfully fabricated by one-step large deformation using a four-column hydraulic machine. The microstructure of the billet is almost completely composed of recrystallized γ grains with large angle boundaries. Tensile testing shows the billet exhibits high tensile strength (780 MPa) and high elongation (1.44%) simultaneously, which benefits from fine γ grains with an average size of 4.9 μm. The ductile–brittle transition temperature is between 750–800 °C.
Effectiveness of Low-Level Laser Therapy during Tooth Movement: A Randomized Clinical Trial
Authors: Isola Gaetano , Matarese Marco , Briguglio Francesco , Grassia Vincenzo , Picciolo Giacomo , Fiorillo Luca , Matarese Giovanni
Published: July 2019

The present study evaluated the effects of low-level laser therapy (LLLT) by means of a diode laser in accelerating orthodontic tooth movement (OTM). After extraction of the first upper premolars for orthodontic purpose, 82 maxillary canines which needed distalization were analyzed in 41 enrolled patients (21 males, 20 females, mean age 13.4 ± 2.1 years). On all experimental sites, an orthodontic force of 50/N was applied by a nickel-titanium (NiTi) closed coil spring (G&H, Franklin, IN, USA) in order to obtain the space closure. Using a split mouth randomized design, the test side was treated using a diode laser (Wiser Laser Doctor Smile, Brendola, Italy) operating at 810 nm wavelength in continuous wave mode at both the buccal and palatal side on three points/side (distal, medial and mesial) (1 W output power, continuous wave of 66.7 J/cm2, energy density of 8 J) at baseline and at 3, 7, and 14 days and every 15 days until the space closed. On the control side, the opposite selected canine was treated only using orthodontic traction. The primary outcome chosen was the overall time needed to complete the levelling and closing space, measured on a study cast. The secondary outcome chosen was the evaluation of pain levels related to tooth traction, using a Visual Analogue Scale (VAS), recorded at 3, 7, and 14 days after treatments. The mean space closures of the maxillary canines were comparable between groups [Test, 4.56 mm (95% CI 3.9–4.8); Control, 4.49 mm (95% CI 3.8–4.7), p = 0.456]. The laser group yielded less mean time [84.35 ± 12.34 days (95% CI 79.3–86)] to accomplish space closure compared to the control group [97.49 ± 11.44 days (91.7–102.3)] (p < 0.001). The test side showed a significant reduction in the average range of dental pain at 3 [Test, 5.41 (95% CI 5.1–5.6); Control, 7.23 (95% CI 6.9–7.6), p < 0.001], 7 [Test, 4.12 (95% CI 3.8–4.7); Control, 5.79 (95% CI 5.4–5.8), p < 0.001], and at 14 days [Test, 2.31 (95% CI 1.8–2.3); Control, 3.84 (95% CI 3.3–4.2), p < 0.001] after treatment (p < 0.001). This study demonstrates that the use of LLLT therapy was effective in accelerating tooth movement and reducing pain levels related to OTM.
Fabrication of 50.0 μm Ultra-Fine Pure Rhodium Wire, Using a Multi-Pass Wire Drawing Process, for Probe Card Pins
Authors: Lee Sang-Kon , Lee In-Kyu , Lee Sung-Yun , Hwang Sun-Kwang
Published: July 2019

Rhodium is a rare material that is widely used in electrical and electronic components due to its excellent mechanical and electrical properties. Ultra-fine rhodium wires in particular are widely used in electronic components. In this study, a multi-pass wire drawing process was designed to fabricate ultra-fine pure rhodium wire with a diameter of 50.0 µm from an initial diameter of 80.0 µm, which is used as probe card pins. An elastic–plastic finite element (FE) analysis was performed to validate the pass schedule that was designed for this study. A fine wire drawing experiment was also carried out to verify the effectiveness of the designed process. As a result, the ultra-fine rhodium wire was fabricated using the design process without wire breaks and the diameter of the final drawn wire was 47.80 µm.
Porous Open-Сell UHMWPE: Experimental Study of Structure and Mechanical Properties
Authors: Salimon Alexei I. , Statnik Eugene S. , Zadorozhnyy Michael Yu. , Senatov Fedor S. , Zherebtsov Dmitry D. , Safonov Alexander A. , Korsunsky Alexander M.
Published: July 2019

Ultra-high molecular weight polyethylene (UHMWPE) is a bioinert polymer that is widely used as bulk material in reconstructive surgery for structural replacements of bone and cartilage. Porous UHMWPE can be used for trabecular bone tissue replacement, and it can be used in living cell studies as bioinert 3D substrate permeable to physiological fluids. It is important to develop techniques to govern the morphology of open-cell porous UHMWPE structures (pore size, shape, and connectivity), since this allows control over proliferation and differentiation in living cell populations. We report experimental results on the mechanical behavior of porous open-cell UHMWPE obtained through sacrificial removal (desalination) of hot-molded UHMWPE-NaCl powder mixtures with pore sizes in the range 75 µm to 500 µm. The structures were characterized using SEM and mechanically tested under static compression and dynamic mechanical analysis (DMA), bending, and tensile tests. Apparent elastic modulus and complex modulus were in the range of 1.2 to 2.5 MPa showing a weak dependence on cell size. Densification under compression caused the apparent elastic modulus to increase to 130 MPa.
An Efficient Beam Element Based on Quasi-3D Theory for Static Bending Analysis of Functionally Graded Beams
Authors: Nguyen Hoang , Hong Tran , Vinh Pham , Thom Do
Published: July 2019

In this paper, a 2-node beam element is developed based on Quasi-3D beam theory and mixed formulation for static bending of functionally graded (FG) beams. The transverse shear strains and stresses of the proposed beam element are parabolic distributions through the thickness of the beam and the transverse shear stresses on the top and bottom surfaces of the beam vanish. The proposed beam element is free of shear-looking without selective or reduced integration. The material properties of the functionally graded beam are assumed to vary according to the power-law index of the volume fraction of the constituents through the thickness of the beam. The numerical results of this study are compared with published results to illustrate the accuracy and convenience rate of the new beam element. The influence of some parametrics on the bending behavior of FGM beams is investigated.
Development and Testing of a Low-Cost Wireless Monitoring System for an Intelligent Tire
Authors: Breglio Giovanni , Irace Andrea , Pugliese Lorenzo , Riccio Michele , Russo Michele , Strano Salvatore , Terzo Mario
Published: July 2019

Intelligent tire concept constitutes one of the approaches to increase the accuracy of active safety systems in vehicle technology. The possibility of detecting tire–road interactions instantaneously has made these systems one of the most important research areas in automotive engineering. This study introduces the use of cost-effective flex and polyvinylidene fluoride strain sensors to estimate some dynamic tire features in free-rolling and real working conditions. The proposed solution combines a microcontroller-based readout circuit for the two sensors with a wireless data transmission system. A suitable prototype was realized and first experimental tests were conducted, in the laboratory as well as on the road. The energy consumption of the wireless monitoring system was optimized. Simulated and experimental results validate the proposed solution.
Fixed Point Results for Multivalued Prešić Type Weakly Contractive Mappings
Authors: Latif Abdul , Nazir Talat , Abbas Mujahid
Published: July 2019

We present fixed points results of multivalued Prešić type k-step iterative mappings satisfying generalized weakly contraction conditions in metric spaces. An example is presented to support the main result proved herein. The stability of fixed point sets of multivalued Prešić type weakly contractive mappings are also established. Global attractivity result for the class of matrix difference equations is derived as application of the result presented herein. These results generalize and extend various comparable results in the existing literature.
Dependence on the Initial Data for the Continuous Thermostatted Framework
Authors: Carbonaro Bruno , Menale Marco
Published: July 2019

The paper deals with the problem of continuous dependence on initial data of solutions to the equation describing the evolution of a complex system in the presence of an external force acting on the system and of a thermostat, simply identified with the condition that the second order moment of the activity variable (see Section 1) is a constant. We are able to prove that these solutions are stable with respect to the initial conditions in the Hadamard’s sense. In this connection, two remarks spontaneously arise and must be carefully considered: first, one could complain the lack of information about the “distance” between solutions at any time t ∈ [ 0 , + ∞ ) ; next, one cannot expect any more complete information without taking into account the possible distribution of the transition probabiliy densities and the interaction rates (see Section 1 again). This work must be viewed as a first step of a research which will require many more steps to give a sufficiently complete picture of the relations between solutions (see Section 5).
An Efficient Derivative Free One-Point Method with Memory for Solving Nonlinear Equations
Authors: Sharma Janak Raj , Kumar Sunil , Cesarano Clemente
Published: July 2019

We propose a derivative free one-point method with memory of order 1.84 for solving nonlinear equations. The formula requires only one function evaluation and, therefore, the efficiency index is also 1.84. The methodology is carried out by approximating the derivative in Newton’s iteration using a rational linear function. Unlike the existing methods of a similar nature, the scheme of the new method is easy to remember and can also be implemented for systems of nonlinear equations. The applicability of the method is demonstrated on some practical as well as academic problems of a scalar and multi-dimensional nature. In addition, to check the efficacy of the new technique, a comparison of its performance with the existing techniques of the same order is also provided.
Linear Maps in Minimal Free Resolutions of Stanley-Reisner Rings
Authors: Katthän Lukas
Published: July 2019

In this short note we give an elementary description of the linear part of the minimal free resolution of a Stanley-Reisner ring of a simplicial complex Δ . Indeed, the differentials in the linear part are simply a compilation of restriction maps in the simplicial cohomology of induced subcomplexes of Δ . Along the way, we also show that if a monomial ideal has at least one generator of degree 2, then the linear strand of its minimal free resolution can be written using only ± 1 coefficients.
On the Stanley Depth of Powers of Monomial Ideals
Authors: Seyed Fakhari S. A.
Published: July 2019

In 1982, Stanley predicted a combinatorial upper bound for the depth of any finitely generated multigraded module over a polynomial ring. The predicted invariant is now called the Stanley depth. Duval et al. found a counterexample for Stanley’s conjecture, and their counterexample is a quotient of squarefree monomial ideals. On the other hand, there is evidence showing that Stanley’s inequality can be true for high powers of monomial ideals. In this survey article, we collect the recent results in this direction. More precisely, we investigate the Stanley depth of powers, integral closure of powers, and symbolic powers of monomial ideals.
Cyanobacteria as Nanogold Factories II: Chemical Reactivity and anti-Myocardial Infraction Properties of Customized Gold Nanoparticles Biosynthesized by Cyanothece sp.
Authors: Younis Nancy S. , Bakir Esam M. , Mohamed Maged E. , El Semary Nermin A.
Published: July 2019

Cyanothece sp., a coccoid, unicellular, nitrogen-fixing and hydrogen-producing cyanobacterium, has been used in this study to biosynthesize customized gold nanoparticles under certain chemical conditions. The produced gold nanoparticles had a characteristic absorption band at 525–535 nm. Two types of gold nanoparticle, the purple and blue, were formed according to the chemical environment in which the cyanobacterium was grown. Dynamic light scattering was implemented to estimate the size of the purple and blue nanoparticles, which ranged from 80 ± 30 nm and 129 ± 40 nm in diameter, respectively. The highest scattering of laser light was recorded for the blue gold nanoparticles, which was possibly due to their larger size and higher concentration. The appearance of anodic and cathodic peaks in cyclic voltammetric scans of the blue gold nanoparticles reflected the oxidation into gold oxide, followed by the subsequent reduction into the nano metal state. The two produced forms of gold nanoparticles were used to treat isoproterenol-induced myocardial infarction in experimental rats. Both forms of nanoparticles ameliorated myocardial infarction injury, with a slight difference in their curative activity with the purple being more effective. Mechanisms that might explain the curative effect of these nanoparticles on the myocardial infarction were proposed. The morphological, physiological, and biochemical attributes of the Cyanothece sp. cyanobacterium were fundamental for the successful production of “tailored” nanoparticles, and complemented the chemical conditions for the differential biosynthesis process. The present research represents a novel approach to manipulate cyanobacterial cells towards the production of different-sized gold nanoparticles whose curative impacts vary accordingly. This is the first report on that type of manipulated gold nanoparticles biosynthesis which will hopefully open doors for further investigations and biotechnological applications.
Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line
Authors: Mendez-Encinas Mayra A. , Carvajal-Millan Elizabeth , Rascón-Chu Agustín , Astiazarán-García Humberto , Valencia-Rivera Dora E. , Brown-Bojorquez Francisco , Alday Efrain , Velazquez Carlos
Published: July 2019

Background and objectives: Arabinoxylans (AX) can gel and exhibit antioxidant capacity. Previous studies have demonstrated the potential application of AX microspheres as colon-targeted drug carriers. However, the cytotoxicity of AX gels has not been investigated so far. Therefore, the aim of the present study was to prepare AX-based particles (AXM) by coaxial electrospraying method and to investigate their antioxidant potential and cytotoxicity on human colon cells. Materials and Methods: The gelation of AX was studied by monitoring the storage (G′) and loss (G′′) moduli. The morphology of AXM was evaluated using optical and scanning electron microscopy (SEM). The in vitro antioxidant activity of AX before and after gelation was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. In addition, the effect of AX and AXM on the proliferation of human colon cells (CCD 841 CoN) was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The final G′ and G′′ values for AX gels were 293 and 0.31 Pa, respectively. AXM presented spherical shape and rough surface with a three-dimensional and porous network. The swelling ratio and mesh size of AXM were 35 g water/g AX and 27 nm, respectively. Gelation decreased the antioxidant activity of AX by 61–64 %. AX and AXM did not affect proliferation or show any toxic effect on the normal human colon cell line CCD 841 CoN. Conclusion: The results indicate that AXM could be promising biocompatible materials with antioxidant activity.
Evaluation of Continuous Renal Replacement Therapy and Therapeutic Plasma Exchange, in Severe Sepsis or Septic Shock in Critically Ill Children
Authors: Aygün Fatih , Varol Fatih , Durak Cansu , Talip Petmezci Mey , Kacar Alper , Dursun Hasan , Irdem Ahmet , Çokuğraş Haluk , Camcıoğlu Yıldız , Çam Halit
Published: July 2019

Background and objective: Severe sepsis and septic shock are life-threatening organ dysfunctions and causes of death in critically ill patients. The therapeutic goal of the management of sepsis is restoring balance to the immune system and fluid balance. Continuous renal replacement therapy (CRRT) is recommended in septic patients, and it may improve outcomes in patients with severe sepsis or septic shock. Therapeutic plasma exchange (TPE) is another extracorporeal procedure that can improve organ function by decreasing inflammatory and anti-fibrinolytic mediators and correcting haemostasis by replenishing anticoagulant proteins. However, research about sepsis and CRRT and TPE in children has been insufficient and incomplete. Therefore, we investigated the reliability and efficacy of extracorporeal therapies in paediatric patients with severe sepsis or septic shock. Materials and methods: We performed a multicentre retrospective study using data from all patients aged <18 years who were admitted to two paediatric intensive care units. Demographic data and reason for hospitalization were recorded. In addition, vital signs, haemogram parameters, and biochemistry results were recorded at 0 h and after 24 h of CRRT. Patients were compared according to whether they underwent CRRT or TPE; mortality between the two treatment groups was also compared. Results: Between January 2014 and April 2019, 168 septic patients were enrolled in the present study. Of them, 47 (27.9%) patients underwent CRRT and 24 underwent TPE. In patients with severe sepsis, the requirement for CRRT was statistically associated with mortality (p < 0.001). In contrast, the requirement for TPE was not associated with mortality (p = 0.124). Conclusion: Our findings revealed that the requirement for CRRT in patients with severe sepsis is predictive of increased mortality. CRRT and TPE can be useful techniques in critically ill children with severe sepsis. However, our results did not show a decrease of mortality with CRRT and TPE.
Association of NOTCH3 Gene Polymorphisms with Ischemic Stroke and Its Subtypes: A Meta-Analysis
Authors: Keat Wei Loo , R. Griffiths Lyn , Looi Irene , Wee Kooi Cheah
Published: July 2019

Background and objectives: NOTCH3 gene variations play a significant role in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). However, the role of NOTCH3 gene polymorphisms in the risk of ischemic stroke, and its subtypes such as atherothrombotic or lacunar strokes, remains unclear. Aims: Hence, we carried out a meta-analysis to examine whether the NOTCH3 rs1043994, rs1044009 and rs3815188 polymorphisms are associated with ischemic stroke and its major subtypes. Materials and Methods: All relevant studies were systematically screened and meta-analyzed using Review Manager (Revman) version 5.3. The strength of the association between NOTCH3 polymorphisms and ischemic stroke risk and its subtypes were measured as odds ratios and 95% confidence intervals, under different genetic models. Results: A total of ten studies were identified, five of which considered NOTCH3 rs1043994 (2077 cases/2147 controls), five of which considered NOTCH3 rs1044009 (2315 cases/3053 controls), and nine of which considered NOTCH3 rs3815188 (2819 cases/2769 controls). These studies were meta-analyzed for their association with ischemic stroke risk. Four studies (874 cases/2002 controls) of the NOTCH3 rs3815188 polymorphism and three studies of the NOTCH3 rs1043994 (643 cases/1552 controls) polymorphism were meta-analyzed for lacunar stroke risk. Three studies (1013 cases/1972 controls) of the NOTCH3 rs3815188 polymorphism were meta-analyzed for atherothrombotic stroke risk. The meta-analysis results showed a lack of association between all of the studied polymorphisms and the risk of ischemic stroke and its major subtypes (i.e., atherothrombotic and lacunar). Conclusions: NOTCH3 polymorphisms are not significantly associated with the risk of ischemic stroke and its subtypes (p < 0.05).
Mirtazapine for the Treatment of Chronic Pruritus
Authors: Khanna Raveena , Boozalis Emily , Belzberg Micah , Zampella John G. , Kwatra Shawn G.
Published: July 2019

Background: Chronic pruritus is a debilitating condition associated with a wide range of dermatologic, systemic and psychogenic etiologies. In patients with chronic pruritus that is refractory to conventional therapy, symptoms can significantly decrease quality of life by contributing to anxiety, sleep disturbances, and in many cases depression. Recent studies have demonstrated the effectiveness of mirtazapine in relieving chronic itch that is refractory to standard first-line therapies. Methods: We searched PubMed for English-language articles containing the words (“pruritus” or “itch”) AND “antidepressant” and then conducted a systematic review of the current literature to summarize the efficacy of mirtazapine in treating chronic itch. Results: All studies reported a reduction in itch intensity following the administration of mirtazapine. Conclusion: Collectively, these studies suggest the potential for mirtazapine to relieve chronic itch attributed to dermatological causes and malignancies. As, such mirtazapine may be an option for patients with chronic pruritus that is refractory to typical first-line treatments.
A Resonant Pressure Sensor Based upon Electrostatically Comb Driven and Piezoresistively Sensed Lateral Resonators
Authors: Shi Xiaoqing , Zhang Sen , Chen Deyong , Wang Junbo , Chen Jian , Xie Bo , Lu Yulan , Li Yadong
Published: July 2019

This study proposes a microfabricated resonant pressure sensor in which a pair of double-ended tuning forks were utilized as resonators where comb electrodes and single-crystal silicon-based piezoresistors were used for electrostatic excitation and piezoresistive detection, respectively. In operations, pressures under measurements deform the pressure-sensitive diaphragm to cause stress variations of two resonators distributed on the central and side positions of the pressure-sensitive diaphragm, where the corresponding changes of the intrinsic resonant frequencies are then captured piezoresistively. The developed resonant pressure sensors were fabricated based on MEMS with open-loop and closed-loop characterizations conducted. Key sensing parameters including quality factors, differential pressure/temperature sensitivities and fitting errors were quantified as higher than 17,000, 48.24 Hz/kPa, 0.15 Hz/°C and better than 0.01% F.S. (140 kpa), respectively. In comparison to previously reported resonant pressure sensors driven by parallel-plate electrodes, the developed sensor in this study is featured with a lower temperature sensitivity and a higher stability.
Dynamic Gene Network Analysis of Caco-2 Cell Response to Shiga Toxin-Producing Escherichia coli-Associated Hemolytic–Uremic Syndrome
Authors: Bando Silvia Y. , Iamashita Priscila , Silva Filipi N. , Costa Luciano da F. , Abe Cecilia M. , Bertonha Fernanda B. , Guth Beatriz E. C. , Fujita André , Moreira-Filho Carlos A.
Published: July 2019

Shiga toxin-producing Escherichia coli (STEC) O113:H21 strains are associated with human diarrhea and some strains may cause hemolytic–uremic syndrome (HUS). In Brazil, these strains are commonly found in cattle but, so far, were not isolated from HUS patients. Here, a system biology approach was used to investigate the differential transcriptomic and phenotypic responses of enterocyte-like Caco-2 cells to two STEC O113:H21 strains with similar virulence factor profiles (i.e., expressing stx2, ehxA, epeA, espA, iha, saa, sab, and subA): EH41 (Caco-2/EH41), isolated from a HUS patient in Australia, and Ec472/01 (Caco-2/Ec472), isolated from bovine feces in Brazil, during a 3 h period of bacteria–enterocyte interaction. Gene co-expression network analysis for Caco-2/EH41 revealed a quite abrupt pattern of topological variation along 3 h of enterocyte–bacteria interaction when compared with networks obtained for Caco-2/Ec472. Transcriptional module characterization revealed that EH41 induces inflammatory and apoptotic responses in Caco-2 cells just after the first hour of enterocyte–bacteria interaction, whereas the response to Ec472/01 is associated with cytoskeleton organization at the first hour, followed by the expression of immune response modulators. Scanning electron microscopy showed more intense microvilli destruction in Caco-2 cells exposed to EH41 when compared to those exposed to Ec472/01. Altogether, these results show that EH41 expresses virulence genes, inducing a distinctive host cell response, and is likely associated with severe pathogenicity.
At-Line Monitoring of the Extraction Process of Rosmarini Folium via Wet Chemical Assays, UHPLC Analysis, and Newly Developed Near-Infrared Spectroscopic Analysis Methods
Authors: Delueg Stefanie , Kirchler Christian G. , Meischl Florian , Ozaki Yukihiro , Popp Michael A. , Bonn Günther K. , Huck Christian W.
Published: July 2019

The present study demonstrates the applicability of at-line monitoring of the extraction process of Rosmarinus officinalis L. leaves (Rosmarini folium) and the development of near-infrared (NIR) spectroscopic analysis methods. Therefore, whole dried Rosmarini folium samples were extracted by maceration with 70% (v/v) ethanol. For the experimental design three different specimen-taking plans were chosen. At first, monitoring was carried out using three common analytical methods: (a) total hydroxycinnamic derivatives according to the European Pharmacopoeia, (b) total phenolic content according to Folin–Ciocalteu, and (c) rosmarinic acid content measured by UHPLC-UV analysis. Precision validation of the wet chemical assays revealed a repeatability of (a) 0.12% relative standard deviation (RSD), (b) 1.1% RSD, and (c) 0.28% RSD, as well as an intermediate precision of (a) 4.1% RSD, (b) 1.3% RSD, and (c) 0.55% RSD. The collected extracts were analyzed with a NIR spectrometer using a temperature-controlled liquid attachment. Samples were measured in transmission mode with an optical path length of 1 mm. The combination of the recorded spectra and the previously obtained analytical reference values in conjunction with multivariate data analysis enabled the successful establishment of partial least squares regression (PLSR) models. Coefficients of determination (R2) were: (a) 0.94, (b) 0.96, and (c) 0.93 (obtained by test-set validation). Since Pearson correlation analysis revealed that the reference analyses correlated with each other just one of the PSLR models is required. Therefore, it is suggested that PLSR model (b) be used for monitoring the extraction process of Rosmarini folium. The application of NIR spectroscopy provides a fast and non-invasive alternative analysis method, which can subsequently be implemented for on- or in-line process control.
Application of Near-infrared Spectroscopy and Multiple Spectral Algorithms to Explore the Effect of Soil Particle Sizes on Soil Nitrogen Detection
Authors: Xiao Shupei , He Yong
Published: July 2019

Soil nitrogen is the key parameter supporting plant growth and development; it is also the material basis of plant growth. An accurate grasp of soil nitrogen information is the premise of scientific fertilization in precision agriculture, where near-infrared (NIR) spectroscopy is widely used for rapid detection of soil nutrients. In this study, the variation law of soil NIR reflectivity spectra with soil particle sizes was studied. Moreover, in order to precisely study the effect of particle size on soil nitrogen detection by NIR, four different spectra preprocessing methods and five different chemometric modeling methods were used to analyze the soil NIR spectra. The results showed that the smaller the soil particle sizes, the stronger the soil NIR reflectivity spectra. Besides, when the soil particle sizes ranged 0.18–0.28 mm, the soil nitrogen prediction accuracy was the best based on the partial least squares (PLS) model with the highest Rp2 of 0.983, the residual predictive deviation (RPD) of 6.706. The detection accuracy was not ideal when the soil particle sizes were too big (1–2 mm) or too small (0–0.18 mm). In addition, the relationship between the mixing spectra of six different soil particle sizes and the soil nitrogen detection accuracy was studied. It was indicated that the larger the gap between soil particle sizes, the worse the accuracy of soil nitrogen detection. In conclusion, soil nitrogen detection precision was affected by soil particle sizes to a large extent. It is of great significance to optimize the pre-treatments of soil samples to realize rapid and accurate detection by NIR spectroscopy.
Sonochemically-Promoted Preparation of Silica-Anchored Cyclodextrin Derivatives for Efficient Copper Catalysis
Authors: Martina Katia , Calsolaro Federica , Zuliani Alessio , Berlier Gloria , Chávez-Rivas Fernando , Moran Maria Jesus , Luque Rafael , Cravotto Giancarlo
Published: July 2019

Silica-supported metallic species have emerged as valuable green-chemistry catalysts because their high efficiency enables a wide range of applications, even at industrial scales. As a consequence, the preparation of these systems needs to be finely controlled in order to achieve the desired activity. The present work presents a detailed investigation of an ultrasound-promoted synthetic protocol for the grafting of β-cyclodextrin (β-CD) onto silica. Truly, ultrasound irradiation has emerged as a fast technique for promoting efficient derivatization of a silica surface with organic moieties at low temperature. Three different β-CD silica-grafted derivatives have been obtained, and the ability of β-CD to direct and bind Cu when CD is bonded to silica has been studied. A detailed characterization has been performed using TGA, phenolphthalein titration, FT-IR, diffuse reflectance (DR), DR UV-Vis, as well as the inductively-coupled plasma (ICP) of the β-CD silica-grafted systems and the relative Cu-supported catalysts. Spectroscopic characterization monitored the different steps of the reaction, highlighting qualitative differences in the properties of amino-derivatized precursors and final products. In order to ensure that the Cu-β-CD silica catalyst is efficient and robust, its applicability in Cu(II)-catalyzed alkyne azide reactions in the absence of a reducing agent has been explored. The presence of β-CD and an amino spacer has been shown to be crucial for the reactivity of Cu(II), when supported.
Conformational and Tautomeric Control by Supramolecular Approach in Ureido-N-iso-propyl,N’-4-(3-pyridin-2-one)pyrimidine
Authors: Kwiatkowski Adam , Kolehmainen Erkki , Ośmiałowski Borys
Published: July 2019

Ureido-N-iso-propyl,N’-4-(3-pyridin-2-one)pyrimidine (1) and its 2-methoxy pyridine derivative (1Me) has been designed and prepared. The conformational equilibrium in urea moiety and tautomerism in the pyrimidine part have been investigated by variable temperature and 1H NMR titrations as well as DFT quantum chemical calculations. The studied compounds readily associate by triple hydrogen bonding with 2-aminonaphthyridine (A) and/or 2,6-bis(acetylamino)pyridine (B). In 1, the proton is forced to 1,3-tautomeric shift upon stimuli and keeps it position, even when one of the partners in the complex was replaced by another molecule. The observed tautomerism controlled by conformational state (kinetic trapping effect) opens new possibilities in molecular sensing that are based on the fact that reverse reaction is not preferred.
Study on the Synthetic Characteristics of Biomass-Derived Isosorbide-Based Poly(arylene ether ketone)s for Sustainable Super Engineering Plastic
Authors: Park Seul-A , Im Changgyu , Oh Dongyeop X. , Hwang Sung Yeon , Jegal Jonggeon , Kim Ji Hyeon , Chang Young-Wook , Jeon Hyeonyeol , Park Jeyoung
Published: July 2019

Demand for the development of novel polymers derived from biomass that can replace petroleum resources has been increasing. In this study, biomass-derived isosorbide was used as a monomer in the polymerization of poly(arylene ether ketone)s, and its synthetic characteristics were investigated. As a phase-transfer catalyst, crown ether has increased the weight-average molecular weight of polymers over 100 kg/mol by improving the reaction efficiency of isosorbide and minimizing the effect of moisture. By controlling the experimental parameters such as halogen monomer, polymerization solvent, time, and temperature, the optimal conditions were found to be fluorine-type monomer, dimethyl sulfoxide, 24 h, and 155 °C, respectively. Biomass contents from isosorbide-based polymers were determined by nuclear magnetic resonance and accelerator mass spectroscopy. The synthesized polymer resulted in a high molecular weight that enabled the preparation of transparent polymer films by the solution casting method despite its weak thermal degradation stability compared to aromatic polysulfone. The melt injection molding process was enabled by the addition of plasticizer. The tensile properties were comparable or superior to those of commercial petrochemical specimens of similar molecular weight. Interestingly, the prepared specimens exhibited a significantly lower coefficient of thermal expansion at high temperatures over 150 °C compared to polysulfone.