bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative evaluation of pain perception with a new needle-free system and dental needle method in children: a randomized clinical trial
Authors: Altan Halenur , Belevcikli Melek , Coşgun Alem , Demir Osman
Published: December 2021

Background

Pain control during dental procedures is one of the most important topics related to behavior management in children. This study aims to compare the pain perception associated with a needle-free system (Comfort-In™) and the dental needle method during filling and pulpotomy treatments in children.

Methods

The study included teeth that required treatment (pulpotomy or filling treatment) in 56 patients aged 4 to 11 years with no systemic problems or history of allergy. Patients were randomly divided into the needle-free system group (filling treatment, n = 13; pulpotomy, n = 15) and dental needle method group (filling treatment, n = 14; pulpotomy, n = 14). For pulpotomy and filling treatment performed with 0.3 mL anesthesia, the active ingredient of which is 2% lidocaine and 1/80000 epinephrine. The patients’ behavior before the procedure was evaluated by a pediatric dentist using the Frankl Behavior Scale. The pain intensity was assessed Immediately after injection (induction), during treatment (treatment), and at the end of the treatment (post treatment) by the Wong-Baker Faces Pain Scale.

Results

The median (IQR-InterQuartile Range) induction pain value was 6[3-8] and 2[0-4] in dental needle method and needle-free system respectively, p < 0.001). In filling and pulpotomy treatment group, no difference between the needle and needle-free group for treatment and post-treatment pain values.

Conclusions

For pulpotomy and filling treatment, needle-free system performed with 0.3 mL anesthesia was found as effective as infiltrative anesthesia with a dental needle method.

Trial registration

ClinicalTrials.gov, NCT04653974. Registered 4 December 2020 – Retrospectively registered.
A conceptual analysis of older adults’ empowerment in contemporary japanese culture
Authors: Tsubouchi Yoshihito , Yorozuya Kyosuke , Tainosyo Akiyoshi , Naito Yasuo
Published: December 2021

Background

Empowerment among older adults is a key concept for improving their health. In contrast, empowerment evolves according to cultural and historical contexts and needs to be consistently tested and constructed. The purpose of this study was to clarify the components of older adults’ empowerment in contemporary Japan and to reconstruct the definition of empowerment.

Methods

A conceptual analysis was performed using Rodgers’ evolutionary method. The data sources were PubMed, Cumulative Index to Nursing and Allied Health Literature, Web of Science, Cochrane Library, and Igaku Chuo Zasshi. The search keywords were “empowerment,” “older adults,” and “Japan/Japanese.” Of the 8811 articles published between 2000 and 2019 that focused on older adults’ empowerment, we selected 60 articles that met our objectives.

Results

Seven antecedents, six attributes, and seven consequences were identified. Older adults’ empowerment in contemporary Japan was defined as “the series of processes in which disclosing oneself, not only verbally but also nonverbally (e.g., through work, roles, and collaborative activities), in connection with others, objectively perceiving one’s existence and challenges, taking proactive actions based on decision-making, and utilizing one’s strengths in new work and community life.”

Conclusions

This concept is useful in practice, education, and research on community development and providing support for older adults based on self-help and mutual aid, not only in Japan but also for the global aging society.
Differences in pregnancy and perinatal outcomes among symptomatic versus asymptomatic COVID-19-infected pregnant women: a systematic review and meta-analysis
Authors: Khan Durray Shahwar A. , Hamid La-Raib , Ali Anna , Salam Rehana A. , Zuberi Nadeem , Lassi Zohra S. , Das Jai K.
Published: December 2021

Background

There is dearth of information on COVID-19’s impact on pregnant women. However, literature reported trends of COVID-19 differ, depending on the presence of clinical features upon presentation.

Objective

This systematic review aimed to assess differences in risk factors, management, complications, and pregnancy and perinatal outcomes in symptomatic vs. asymptomatic pregnant women with confirmed SARS-CoV-2 infection.

Methods

A search was run on electronic databases to identify studies reporting COVID-19 in pregnancy. Meta-analysis was performed and odds ratios and mean difference with 95% confidence intervals were calculated using Review Manager 5.4. Review Prospero registration number CRD42020204662.

Results

We included ten articles reporting data from 3158 pregnancies; with 1900 symptomatic and 1258 asymptomatic pregnant women. There was no significant difference in the mean age, gestational age, and body mass index between the two groups. The meta-analysis suggested that pregnant women who were obese (OR:1.37;95%CI:1.15 to 1.62), hypertensive (OR:2.07;95%CI:1.38 to 3.10) or had a respiratory disorder (OR:1.64;95%CI:1.25 to 2.16), were more likely to be symptomatic when infected with SARS-CoV-2. Pregnant women with Black (OR:1.48;95%CI:1.19 to 1.85) or Asian (OR:1.64;95%CI:1.23 to 2.18) ethnicity were more likely to be symptomatic while those with White ethnicity (OR:0.63;95%CI:0.52 to 0.76) were more likely to be asymptomatic. Cesarean-section delivery (OR:1.40;95%CI:1.17 to 1.67) was more likely amongst symptomatic pregnant women. The mean birthweight(g) (MD:240.51;95%CI:188.42 to 293.51), was significantly lower, while the odds of low birthweight (OR:1.85;95%CI:1.06 to 3.24) and preterm birth (< 37 weeks) (OR:2.10;95%CI:1.04 to 4.23) was higher amongst symptomatic pregnant women. Symptomatic pregnant women had a greater requirement for maternal ICU admission (OR:13.25;95%CI:5.60 to 31.34) and mechanical ventilation (OR:15.56;95%CI:2.96 to 81.70) while their neonates had a higher likelihood for Neonatal Intensive Care Unit admission (OR:1.96;95%CI:1.59 to 2.43). The management strategies in the included studies were poorly discussed, hence could not be analyzed.

Conclusion

The evidence suggests that the presence of risk factors (co-morbidities and ethnicity) increased the likelihood of pregnant women being symptomatic. Higher odds of complications were also observed amongst symptomatic pregnant women. However, more adequately conducted studies with adjusted analysis and parallel comparison groups are required to reach conclusive findings.
Acromiohumeral distance and supraspinatus tendon thickness in people with shoulder impingement syndrome compared to asymptomatic age and gender-matched participants: a case control study
Authors: Hunter Donald J. , Rivett Darren A. , McKiernan Sharmaine , Snodgrass Suzanne J.
Published: December 2021

Background

Shoulder impingement syndrome (SIS) is the most common form of shoulder pain. Conservative and surgical treatments for SIS are often not effective. One such surgical intervention is subacromial decompression, aimed at widening the subacromial space (SAS). A better understanding of the changes in the SAS may help explain the relative ineffectiveness of current interventions.

Objective: To measure the acromiohumeral distance (AHD) and supraspinatus tendon thickness (STT) in people with SIS using a case control study.

Methods

The AHD and STT of 39 participants with SIS ≥3 months and 39 age, gender and dominant arm matched controls were measured using ultrasound imaging. Between-group differences for AHD and STT were compared using t-tests. A linear regression was used to determine if there was a relationship between AHD and STT measures, with group as a covariate.

Results

Compared to controls (mean age 55.7 years, SD 10.6), individuals with SIS (mean age 57.1 years, SD 11.1) had a significantly larger AHD (mean difference 2.14 mm, 95% CI 1.21, 3.07, p < 0.001) and STT (mean difference 1.25 mm, 95% CI 0.60, 1.90, p < 0.001). The linear regression model indicated an association between AHD and STT (β = 0.59, 95% CI 0.29, 0.89, p < 0.01, R2 = 0.35, n = 78), suggesting that as STT increases in size, so does the AHD.

Conclusion

Individuals with SIS had a larger AHD and greater STT than controls. These results suggest the SAS is already wider in people with SIS and that the symptoms associated with SIS may be more related to an increased STT than a smaller SAS.
Ensemble learning for the early prediction of neonatal jaundice with genetic features
Authors: Deng Haowen , Zhou Youyou , Wang Lin , Zhang Cheng
Published: December 2021

Background

Neonatal jaundice may cause severe neurological damage if poorly evaluated and diagnosed when high bilirubin occurs. The study explored how to effectively integrate high-dimensional genetic features into predicting neonatal jaundice.

Methods

This study recruited 984 neonates from the Suzhou Municipal Central Hospital in China, and applied an ensemble learning approach to enhance the prediction of high-dimensional genetic features and clinical risk factors (CRF) for physiological neonatal jaundice of full-term newborns within 1-week after birth. Further, sigmoid recalibration was applied for validating the reliability of our methods.

Results

The maximum accuracy of prediction reached 79.5% Area Under Curve (AUC) by CRF and could be marginally improved by 3.5% by including genetic variant (GV). Feature importance illustrated that 36 GVs contributed 55.5% in predicting neonatal jaundice in terms of gain from splits. Further analysis revealed that the main contribution of GV was to reduce the false-positive rate, i.e., to increase the specificity in the prediction.

Conclusions

Our study shed light on the theoretical and practical value of GV in the prediction of neonatal jaundice.
Models of COVID-19 vaccine prioritisation: a systematic literature search and narrative review
Authors: Saadi Nuru , Chi Y-Ling , Ghosh Srobana , Eggo Rosalind M. , McCarthy Ciara V. , Quaife Matthew , Dawa Jeanette , Jit Mark , Vassall Anna
Published: December 2021

Background

How best to prioritise COVID-19 vaccination within and between countries has been a public health and an ethical challenge for decision-makers globally. We reviewed epidemiological and economic modelling evidence on population priority groups to minimise COVID-19 mortality, transmission, and morbidity outcomes.

Methods

We searched the National Institute of Health iSearch COVID-19 Portfolio (a database of peer-reviewed and pre-print articles), Econlit, the Centre for Economic Policy Research, and the National Bureau of Economic Research for mathematical modelling studies evaluating the impact of prioritising COVID-19 vaccination to population target groups. The first search was conducted on March 3, 2021, and an updated search on the LMIC literature was conducted from March 3, 2021, to September 24, 2021. We narratively synthesised the main study conclusions on prioritisation and the conditions under which the conclusions changed.

Results

The initial search identified 1820 studies and 36 studies met the inclusion criteria. The updated search on LMIC literature identified 7 more studies. 43 studies in total were narratively synthesised. 74% of studies described outcomes in high-income countries (single and multi-country). We found that for countries seeking to minimise deaths, prioritising vaccination of senior adults was the optimal strategy and for countries seeking to minimise cases the young were prioritised. There were several exceptions to the main conclusion, notably that reductions in deaths could be increased if groups at high risk of both transmission and death could be further identified. Findings were also sensitive to the level of vaccine coverage.

Conclusion

The evidence supports WHO SAGE recommendations on COVID-19 vaccine prioritisation. There is, however, an evidence gap on optimal prioritisation for low- and middle-income countries, studies that included an economic evaluation, and studies that explore prioritisation strategies if the aim is to reduce overall health burden including morbidity.
Prevalence, co-infection and seasonality of fecal enteropathogens from diarrheic cats in the Republic of Korea (2016–2019): a retrospective study
Authors: Oh Ye-In , Seo Kyoung-Won , Kim Do-Hyung , Cheon Doo-Sung
Published: December 2021

Background

Diarrhea is one of the most common clinical symptoms in cats and can be caused by infectious pathogens and investigation of the prevalence, co-infection and seasonality of enteropathogens are not well-established in diarrheic cats.

Results

Fecal samples of 1620 diarrheic cats were collected and enteropathogens were detected using real-time PCR. We retrospectively investigated the clinical features, total/seasonal prevalence, and infection patterns of enteropathogens. The positive infection rate was 82.59%. Bacterial, viral, and protozoal infections accounted for 49.3, 37.57, and 13.13% of cases, respectively. Feline enteric coronavirus (FECV) was the most common pathogen (29.37%), followed byClostridium(C.)perfringens,Campylobacter(C.)coli, feline parvovirus, andTritrichomonas foetus. The seasonality of enteropathogens was observed with peaks as follows: bacterial infections peaked in October, viral infections peaked in November, and protozoal infections peaked in August. Viral and protozoal infections showed differences in prevalence according to patient age. In the infection patterns, the ratios of single infections, mixed infections, and co-infections were 35.72, 9.87, and 54.41%, respectively. FECV was predominant in single infections. The most common patterns of multiple infections wereC. perfringensandC. coliin mixed infections andC. perfringensand FECV in co-infections.

Conclusions

Infection patterns differed according to the enteropathogen species, seasonality, and age distribution in cats. The results of this study might be helpful to understand in clinical characteristics of feline infectious diarrhea. In addition, continued monitoring of feline enteropathogens is required.
Minimum redundancy maximal relevance gene selection of apoptosis pathway genes in peripheral blood mononuclear cells of HIV-infected patients with antiretroviral therapy-associated mitochondrial toxicity
Authors: Bose Eliezer , Paintsil Elijah , Ghebremichael Musie
Published: December 2021

Background

We previously identified differentially expressed genes on the basis of false discovery rate adjusted P value using empirical Bayes moderated tests. However, that approach yielded a subset of differentially expressed genes without accounting for redundancy between the selected genes.

Methods

This study is a secondary analysis of a case–control study of the effect of antiretroviral therapy on apoptosis pathway genes comprising of 16 cases (HIV infected with mitochondrial toxicity) and 16 controls (uninfected). We applied the maximum relevance minimum redundancy (mRMR) algorithm on the genes that were differentially expressed between the cases and controls. The mRMR algorithm iteratively selects features (genes) that are maximally relevant for class prediction and minimally redundant. We implemented several machine learning classifiers and tested the prediction accuracy of the two mRMR genes. We next used network analysis to estimate and visualize the association among the differentially expressed genes. We employed Markov Random Field or undirected network models to identify gene networks related to mitochondrial toxicity. The Spinglass model was used to identify clusters of gene communities.

Results

The mRMR algorithm ranked DFFA and TNFRSF1A, two of the upregulated proapoptotic genes, on the top. The overall prediction accuracy was 86%, the two mRMR genes correctly classified 86% of the participants into their respective groups. The estimated network models showed different patterns of gene networks. In the network of the cases, FASLG was the most central gene. However, instead of FASLG, ABL1 and LTBR had the highest centrality in controls.

Conclusion

The mRMR algorithm and network analysis revealed a new correlation of genes associated with mitochondrial toxicity.
Comprehensive analysis of prognostic immune-related genes and drug sensitivity in cervical cancer
Authors: Pi Ya-Nan , Guo Jun-Nan , Lou Ge , Cui Bin-Bin
Published: December 2021

Background

Cervical cancer (CC) is the leading cause of cancer-related death in women. A limited number of studies have investigated whether immune-prognostic features can be used to predict the prognosis of CC. This study aimed to develop an improved prognostic risk scoring model (PRSM) for CC based on immune-related genes (IRGs) to predict survival and determine the key prognostic IRGs.

Methods

We downloaded the gene expression profiles and clinical data of CC patients from the TCGA and GEO databases. The ESTIMATE algorithm was used to calculate the score for both immune and stromal cells. Differentially expressed genes (DEGs) in different subpopulations were analyzed by “Limma”. A weighted gene co-expression network analysis (WGCNA) was used to establish a DEG co-expression module related to the immune score. Immune-related gene pairs (IRGPs) were constructed, and univariate- and Lasso-Cox regression analyses were used to analyze prognosis and establish a PRSM. A log-rank test was used to verify the accuracy and consistency of the scoring model. Identification of the predicted key IRG was ensured by the application of functional enrichment, DisNor, protein–protein interactions (PPIs) and heatmap. Finally, we extracted the key prognostic immune-related genes from the gene expression data, validated the key genes by immunohistochemistry and analyzed the correlation between their expression and drug sensitivity.

Results

A new PRSM was developed based on 22 IRGPs. The prognosis of the low-risk group in the model group (P < 0.001) and validation group (P = 0.039) was significantly better than that in the high-risk group. Furthermore, M1 and M2 macrophages were highly expressed in the low-risk group. Retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) and the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway were significantly enriched in the low-risk group. Three representative genes (CD80, CD28, and LCP2) were markers of CC prognosis. CD80 and CD28 may more prominent represent important indicators to improve patient prognosis. These key genes was positively correlated with drug sensitivity. Finally, we found that differences in the sensitivity to JNK inhibitors could be distinguished based on the use and risk grouping of this PRSM.

Conclusions

The prognostic model based on the IRGs and key genes have potential clinical significance for predicting the prognosis of CC patients, providing a foundation for clinical prognosis judgment and individualized treatment.
Effect of fecal microbiota transplantation in patients with slow transit constipation and the relative mechanisms based on the protein digestion and absorption pathway
Authors: Xie Lulu , Xu Chen , Fan Yadong , Li Yuwei , Wang Ying , Zhang Xiaoyu , Yu Shuang , Wang Jida , Chai Rundong , Zhao Zeyu , Jin Yutong , Xu Zhe , Zhao Shuwu , Bian Yuhong
Published: December 2021

Background

Fecal microbiota transplantation (FMT) is considered an effective treatment for slow transit constipation (STC); nevertheless, the mechanism remains unclear.

Methods

In this study, eight patients with STC were selected according to the inclusion and exclusion criteria; they then received three treatments of FMT. The feces and serum of STC patients were collected after each treatment and analyzed by integrating 16 s rRNA microbiome and metabolomic analyses.

Results

The results showed that the percentage of clinical improvement reached 62.5% and the rates of patients’ clinical remission achieved 75% after the third treatment. At the same time, FMT improved the Wexner constipation scale (WCS), the Gastrointestinal Quality-of-Life Index (GIQLI) and Hamilton Depression Scale (HAMD). Fecal microbiome alpha diversity and beta diversity altered significantly after FMT. Analysis of the 16 s rRNA microbiome showed that the numbers of Bacteroidetes (Prevotell/Bacteroides) and Firmicute (Roseburia/Blautia) decreased, whereas Actinobacteria (Bifidobacterium), Proteobacteria (Escherichia), and Firmicute (Lactobacillus) increased after FMT. The metabolomics analyses showed that the stool of FMT-treated patients were characterized by relatively high levels of N-Acetyl-L-glutamate, gamma-L-glutamyl-L-glutamic acid, Glycerophosphocholine, et al., after FMT. Compared with baseline, the serum of treated patients was characterized by relatively high levels of L-Arginine, L-Threonine, Ser-Arg, Indoleacrylic acid, Phe-Tyr, 5-L-Glutamyl-L-alanine, and lower levels of Erucamide after the treatment. The correlation analysis between the metabolites and gut microbiota showed a significant correlation. For example, L-Arginine was positively correlated with lactobacillus, et al. L-Threonine was positively correlated with Anaerovibrio, Sediminibacterium but negatively correlated with Phascolarctobacterium. Erucamide had significant negative correlations with Sediminibacterium and Sharpea, while being positively correlated with Phascolarctobacterium. Enriched KEGG pathways analysis demonstrated that the protein digestion and absorption pathways gradually upregulated with the increase of FMT frequency. The L-Arginine and L-Threonine were also involved in the pathway. A large amount of Na + was absorbed in the pathway, so that it might increase mucus secretion and electrical excitability of GI smooth muscle.

Conclusions

Therefore, we speculated that FMT changed the patients’ gut microbiota and metabolites involved in the protein digestion and absorption pathways, thereby improving the symptoms of STC. Study on the effectiveness and safety of FMT in the treatment of STC. The study was reviewed and approved by Ethics Committee of Tianjin People's Hospital (ChiCTR2000033227) in 2020.
Microdialysis and microperfusion electrodes in neurologic disease monitoring
Authors: Stangler Luke A. , Kouzani Abbas , Bennet Kevin E. , Dumee Ludovic , Berk Michael , Worrell Gregory A. , Steele Steven , Burns Terence C. , Howe Charles L.
Published: December 2021

Contemporary biomarker collection techniques in blood and cerebrospinal fluid have to date offered only modest clinical insights into neurologic diseases such as epilepsy and glioma. Conversely, the collection of human electroencephalography (EEG) data has long been the standard of care in these patients, enabling individualized insights for therapy and revealing fundamental principles of human neurophysiology. Increasing interest exists in simultaneously measuring neurochemical biomarkers and electrophysiological data to enhance our understanding of human disease mechanisms. This review compares microdialysis, microperfusion, and implanted EEG probe architectures and performance parameters. Invasive consequences of probe implantation are also investigated along with the functional impact of biofouling. Finally, previously developed microdialysis electrodes and microperfusion electrodes are reviewed in preclinical and clinical settings. Critically, current and precedent microdialysis and microperfusion probes lack the ability to collect neurochemical data that is spatially and temporally coincident with EEG data derived from depth electrodes. This ultimately limits diagnostic and therapeutic progress in epilepsy and glioma research. However, this gap also provides a unique opportunity to create a dual-sensing technology that will provide unprecedented insights into the pathogenic mechanisms of human neurologic disease.
BUB1 drives the occurrence and development of bladder cancer by mediating the STAT3 signaling pathway
Authors: Jiang Ning , Liao Yihao , Wang Miaomiao , Wang Youzhi , Wang Keke , Guo Jianing , Wu Peikang , Zhong Boqiang , Guo Tao , Wu Changli
Published: December 2021

Background

The incidence of bladder urothelial carcinoma (UC), a common malignancy of the urinary tract, is approximately three times higher in men than in women. High expression of the mitotic kinase BUB1 is associated with the occurrence and development of several cancers, although the relationship between BUB1 and bladder tumorigenesis remains unclear.

Methods

Using a microarray approach, we found increased BUB1 expression in human BCa. The association between BUB1 and STAT3 phosphorylation was determined through molecular and cell biological methods. We evaluated the impact of pharmacologic inhibition of BUB1 kinase activity on proliferation and BCa progression in vitro and in vivo.

Results

In this study, we found that BUB1 expression was increased in human bladder cancer (BCa). We further identified through a series of molecular and cell biological approaches that BUB1 interacted directly with STAT3 and mediated the phosphorylation of STAT3 at Ser727. In addition, the findings that pharmacologic inhibition of BUB1 kinase activity significantly suppressed BCa cell proliferation and the progression of bladder cancer in vitro and in vivo were further verified. Finally, we found that the BUB1/STAT3 complex promoted the transcription of STAT3 target genes and that depletion of BUB1 and mutation of the BUB1 kinase domain abrogated this transcriptional activity, further highlighting the critical role of kinase activity in the activation of STAT3 target genes. A pharmacological inhibitor of BUB1 (2OH-BNPP1) was able to significantly inhibit the growth of BCa cell xenografts.

Conclusion

This study showed that the BUB1 kinase drives the progression and proliferation of BCa by regulating the transcriptional activation of STAT3 signaling and may be an attractive candidate for therapeutic targeting in BCa.
Deciphering the nonsense-mediated mRNA decay pathway to identify cancer cell vulnerabilities for effective cancer therapy
Authors: Bongiorno Roberta , Colombo Mario Paolo , Lecis Daniele
Published: December 2021

Nonsense-mediated mRNA decay (NMD) is a highly conserved cellular surveillance mechanism, commonly studied for its role in mRNA quality control because of its capacity of degrading mutated mRNAs that would produce truncated proteins. However, recent studies have proven that NMD hides more complex tasks involved in a plethora of cellular activities. Indeed, it can control the stability of mutated as well as non-mutated transcripts, tuning transcriptome regulation. NMD not only displays a pivotal role in cell physiology but also in a number of genetic diseases. In cancer, the activity of this pathway is extremely complex and it is endowed with both pro-tumor and tumor suppressor functions, likely depending on the genetic context and tumor microenvironment. NMD inhibition has been tested in pre-clinical studies showing favored production of neoantigens by cancer cells, which can stimulate the triggering of an anti-tumor immune response. At the same time, NMD inhibition could result in a pro-tumor effect, increasing cancer cell adaptation to stress. Since several NMD inhibitors are already available in the clinic to treat genetic diseases, these compounds could be redirected to treat cancer patients, pending the comprehension of these variegated NMD regulation mechanisms. Ideally, an effective strategy should exploit the anti-tumor advantages of NMD inhibition and simultaneously preserve its intrinsic tumor suppressor functions. The targeting of NMD could provide a new therapeutic opportunity, increasing the immunogenicity of tumors and potentially boosting the efficacy of the immunotherapy agents now available for cancer treatment.
Long-term cardiovascular complications following sepsis: is senescence the missing link?
Authors: Merdji Hamid , Schini-Kerth Valérie , Meziani Ferhat , Toti Florence
Published: December 2021

Among the long-term consequences of sepsis (also termed “post-sepsis syndrome”) the increased risk of unexplained cardiovascular complications, such as myocardial infarction, acute heart failure or stroke, is one of the emerging specific health concerns. The vascular accelerated ageing also named premature senescence is a potential mechanism contributing to atherothrombosis, consequently leading to cardiovascular events. Indeed, vascular senescence-associated major adverse cardiovascular events (MACE) are a potential feature in sepsis survivors and of the elderly at cardiovascular risk. In these patients, accelerated vascular senescence could be one of the potential facilitating mechanisms. This review will focus on premature senescence in sepsis regardless of age. It will highlight and refine the potential relationships between sepsis and accelerated vascular senescence. In particular, key cellular mechanisms contributing to cardiovascular events in post-sepsis syndrome will be highlighted, and potential therapeutic strategies to reduce the cardiovascular risk will be further discussed.
Anti-diuretic and anti-glycemic properties of Jatropha gossypiifolia L. leave extract on wistar rats
Authors: Gabriel Benjamin Ogunma , Idu Mac Donald
Published: December 2021

Background

Jatropha gossypiifoliaL. is a widespread plant in tropical and sub-tropical countries used in traditional medicine. This study investigated the anti-diuretic and anti-hyperglycemia activities ofJ. gossypiifolialeave extract on streptozotocin-induced diabetic rats.

Methods

The leaves was shade dried, pulverized and prepared into extract. 30, 50 and 100 mg/kg of the leaves extracts ofJ. gossypiifoliawas subject to diuretics and hyperglycemic properties using established protocol of diuretic and diabetes test on the rat bladders emptied via mild compression in the pelvic region and gently pulling of their tails. 0.5 ml/kg normal saline, reference drug and the tested were administered with a single dose of the various drugs, and Streptozotocin (STZ) was freshly prepared in 0.1 M citrate buffer with pH 4.5 prior to induction, animals were fasted 24 h and single dose of 45 mg STZ per kg body weight was administered intraperitoneally. Urine and blood samples were isolated from rats and centrifuged for the determination of renal function test. Diuretic and antidiabetic indexes where evaluated using adopted method.

Results

This study showed that, graded doses of the extract significantly increased diuretic effect, specifically at 100 mg/kg increased diuretic index at 4.29 and urine volume 5.06 and 10 mg/kg Hydrochlorothiazide with 6.23 ml when compared untreated group (1.18 ml) (p < 0.0001). Also, it regulated renal function in homeostatic state. Graded doses at (30, 50 and 100 mg/kg) of the extract significantly reduced streptozotocine induced increased blood glucose level at day 14 (84.00, 60.67 and 42.00 IU/mL) when compared with 20 mg/kg glibenclamide and diabetics control (81.67 and 463.00 IU/mL) (p > 0.05). Also, the extract maintained a normal body mass indexes, biochemical and anatomical structure.

Conclusion

The effect associated withJ. gossypiifoliapotentiated its anti-diuretic and anti-hyperglycemic properties as early stated in the ethnomedicinal reports.
Introduction to this special section: Latin America
Authors: Velásquez Antonio J. , Lupinacci Wagner Moreira , Molinares Carlos Eduardo
Published: December 2021

Recent oil and gas exploration and development projects in Latin America, particularly in offshore basins, have increased the hydrocarbon resources of the region considerably. Geophysical technologies have played a significant role in the growth observed across the region, and those technologies will be crucial in the development of smart energy transition alternatives. That is what inspired this special section, which showcases a variety of technology applications, project scopes, scales, depths of investigation, and techniques, representing the diverse solutions required to tackle subsurface challenges in three major growth geographies: the Brazilian presalt, the Caribbean offshore, and the Gulf of Mexico.
Geostatistics assisted by machine learning for reservoir property modeling: A case study in presalt carbonates of Buzios Field, Brazil
Authors: Ferreira Danilo Jotta Ariza , de Oliveira Gabriella Martins Baptista , Castro Thais Mallet , Dias Raquel Macedo , Lupinacci Wagner Moreira
Published: December 2021

An embedded model estimator (EMBER) petrophysical modeling algorithm has been applied to obtain effective porosity and permeability within the presalt carbonate reservoirs of the Barra Velha Formation in Buzios Field, Santos Basin. This advanced methodology was used due to the heterogeneity and complexity of the reservoirs, which makes modeling by conventional geostatistical methodologies difficult. For effective porosity modeling, we chose one facies model, one stratigraphic seismic attribute (acoustic impedance), and one structural seismic attribute (local flatness) as secondary variables. Permeability was modeled by using the best effective porosity simulation result as a secondary variable. Our results demonstrate that average effective porosity and permeability were 0.10 v/v and 440 md, respectively, indicating good reservoir quality throughout the studied area. A vertical trend of high effective porosities and permeabilities for the basal and uppermost reservoir sections was identified in our results, as well as a trend with lower values for these reservoir properties for the intermediate reservoir section. The lower section of the formation presented more continuity, and we infer it to be the best reservoir interval. We observed two horizontal trends for these reservoir properties at the formation top: one of higher values aligned to the north–south direction at the structural highs and another of lower reservoir properties related to isolated structural lows within structural highs. Correlation between modeled results and the blind test ANP-1 well upscaled properties was high, and upscaled well-log property distributions were preserved in the EMBER simulations, proving the predictive capacity of the algorithm. Finally, conditional distributions analysis indicated that the basal section of the Barra Velha Formation presents higher uncertainty for the estimation of effective porosity. Even though this interval is considered to have the best reservoir characteristics, decision making should be done with caution for this section.
Enhancing subsalt imaging through advanced identification and suppression of interbed multiples and mode-converted reflections — Gulf of Mexico and Brazil case studies
Authors: Alai Riaz , Liu Faqi , Verschuur Eric , Thorbecke Jan , Coskun Gundogan , Dong Shuqian , Macesanu Cosmin , Wang Bin , Slind Christopher Lee , Andersen Eric
Published: December 2021

In our case studies, the success of subsalt exploration and development wells depended heavily on the characterization of highly heterogeneous lacustrine microbial carbonates. Acoustic and elastic inversions have proved to be a good proxy for identification of reservoir quality variation for exploration and development well placements. However, qualitative and quantitative usage of subsalt seismic amplitudes requires proper illumination and good signal-to-noise ratio. If properly imaged, mode-converted reflections and interbed multiples can be complementary to the P-wave image. But, in conventional P-wave-oriented imaging, both types of events cannot be imaged correctly. They appear as coherent noise and negatively impact the overall exploration and development project outcomes, especially in areas with poor illumination. This paper consists of two parts: first, we investigate the potential problems resulting from converted waves and interbed multiples in data from two different basins — the Gulf of Mexico and the Campos Basin in offshore Brazil — and show our approach to attenuate them to reveal the true structures. The second part focuses on advanced identification of interbed multiples in modeling and migration methods. To facilitate the various strategies to attenuate interbed multiples, “interpretation” of the various events plays a significant role. Vertical seismic profile (VSP) data are excellent for the purpose; however, these data are only available at well locations, if they are recorded. As a result of many years of technology advancement, pseudo VSP data can be constructed effectively from standard streamer survey data. Two methods are highlighted in this paper for building pseudo VSP data in a full two-way sense, based on a typical Brazil-type salt model: Marchenko-based processing and full-wavefield migration. Major subsalt plays in the Gulf of Mexico and emerging plays in Brazil should benefit significantly from elimination of these kinds of coherent noise.
Sigmoidal normal faults and evidence for vertical-axis block rotation in an oblique convergent margin: A 3D seismic example from offshore Colombia
Authors: Galindo Pedro A. , Lonergan Lidia
Published: December 2021

Sigmoidal fold and fault geometries are typical kinematic indicators of strike-slip fault zones. We document kilometer-scale, normal faults with sigmoidal plan-view geometries within the dextral pull-apart Bahia Basin, at the rear of the obliquely convergent South Caribbean Deformed Belt, offshore Colombia. Using 3D seismic reflection data calibrated to wells, closely spaced, low-displacement, planar normal faults are mapped within the Miocene strata. A series of seismic horizontal (time) slices and computed seismic attributes are used to interpret the 3D configuration of these faults. The closely spaced faults display an east–west trend with a progressive rotation into a northwest–southeast trend. In map view, the fault traces curve toward their tips, describing a sigmoidal-Z geometry that terminates at discrete northeast–southwest-trending fault zones. The structures observed may correspond to either tension fractures, which form theoretically at 45°, or antithetic shear fractures with normal displacement formed at 50°–70° to the boundaries of a dextral shear zone. These scenarios lead to a clockwise block rotation of between 20° and 40° within the shear zone. This study shows the first example of vertical-axis block rotations observed offshore in the western end of the South Caribbean margin and is an important example of the use of 3D seismic data to identify rotations where paleomagnetic studies are not available.
Research Committee Update: The curmudgeon's column: High-tech low tech
Authors: Popovici Alexander Mihai
Published: December 2021

When I posted to LinkedIn some time back to generate publicity for my Geophysical Society of Houston technical lunch talk on beam tomography, one of the interesting comments from a colleague at a large service company was that beam technology is “a technology with many benefits that unfortunately is commonly regarded as old school/low tech.” I loved receiving the comment because it gives me an opportunity to talk about one of my favorite business concepts that I always look to apply: high-tech low tech. “High tech” is a term for new technology that incorporates advanced features. “Low tech” is old-school technology. I'll start with an example: the story of the Nest thermostat.
Cytoreductive Surgery of the Primary Tumor in Metastatic Adrenocortical Carcinoma: Impact on Patients’ Survival
Authors: Srougi Victor , Bancos Irina , Daher Marilyne , Lee Jeffrey E , Graham Paul H , Karam Jose A , Henriquez Andres , Mckenzie Travis J , Sada Alaa , Bourdeau Isabelle , Poirier Jonathan , Vaidya Anand , Abbondanza Tiffany , Kiernan Colleen M , Rao Sarika N , Hamidi Oksana , Sachithanandan Nirupa , Hoff Ana O , Chambo Jose L , Almeida Madson Q , Habra Mouhammed Amir , Fragoso Maria C B V
Published: December 2021

Context

The role of cytoreduction of adrenocortical carcinoma (ACC) remains poorly understood.

Objective

To analyze the impact of cytoreductive surgery of the primary tumor in patients with metastatic ACC.

Design and Setting

We performed a multicentric, retrospective paired cohort study comparing the overall survival (OS) in patients with metastatic ACC who were treated either with cytoreductive surgery (CR group) or without cytoreductive surgery (no-CR group) of the primary tumor. Data were retrieved from 9 referral centers in the American-Australian-Asian Adrenal Alliance collaborative research group.

Patients

Patients aged ≥18 years with metastatic ACC at initial presentation who were treated between January 1, 1995, and May 31, 2019.

Intervention

Performance (or not) of cytoreductive surgery of the primary tumor.

Main outcome and measures

A propensity score match was done using age and the number of organs with metastasis (≤2 or >2). The main outcome was OS, determined from the date of diagnosis until death or until last follow-up for living patients.

Results

Of 339 patients pooled, 239 were paired and included: 128 in the CR group and 111 in the no-CR group. The mean follow-up was 67 months. Patients in the no-CR group had greater risk of death than did patients in the CR group (hazard ratio [HR] = 3.18; 95% CI, 2.34-4.32). Independent predictors of survival included age (HR = 1.02; 95% CI, 1.00-1.03), hormone excess (HR = 2.56; 95% CI, 1.66-3.92), and local metastasis therapy (HR = 0.41; 95% CI, 0.47-0.65).

Conclusion

Cytoreductive surgery of the primary tumor in patients with metastatic ACC is associated with prolonged survival.
Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors
Authors: Titialii-Torres Kayla F. , Morris Ann C.
Published: December 2021

Diabetes is linked to various long-term complications in adults, such as neuropathy, nephropathy and diabetic retinopathy. Diabetes poses additional risks for pregnant women, because glucose passes across the placenta, and excess maternal glucose can result in diabetic embryopathy. While many studies have examined the teratogenic effects of maternal diabetes on fetal heart development, little is known about the consequences of maternal hyperglycemia on the development of the embryonic retina. To address this question, we investigated retinal development in two models of embryonic hyperglycemia in zebrafish. Strikingly, we found that hyperglycemic larvae displayed a significant reduction in photoreceptors and horizontal cells, whereas other retinal neurons were not affected. We also observed reactive gliosis and abnormal optokinetic responses in hyperglycemic larvae. Further analysis revealed delayed retinal cell differentiation in hyperglycemic embryos that coincided with increased reactive oxygen species (ROS). Our results suggest that embryonic hyperglycemia causes abnormal retinal development via altered timing of cell differentiation and ROS production, which is accompanied by visual defects. Further studies using zebrafish models of hyperglycemia will allow us to understand the molecular mechanisms underlying these effects.
Παρασκευή και ιδιότητες μικροσωματιδίων σε οργανωμένες μοριακές φάσεις
Authors: Παπουτσή Δέσποινα
Published: December 2021

Το αντικείμενο της παρούσας διδακτορικής διατριβής είναι η παρασκευή νανοσωματιδίων τιτάνιας μέσα σε περιορισμένο περιβάλλον που δημιουργείται από ανάστροφα μικήλλια. Οι μέθοδοι μελέτης που χρησιμοποιήθηκαν ήταν φάσματα απορρόφησης και στατικού φθορισμού, χρονική ανάλυση φθορισμού και μικροσκοπία σαρώσεως με ακίδα. Στη χρονική ανάλυση φθορισμού χρησιμοποιήθηκε το μοντέλο των τεταμένων εκθετικών που είναι βασισμένο στη θεωρία των περιορισμένων διαστάσεων. Αρχικά, μελετήσαμε ένα πρότυπο σύστημα υπερμοριακών σχηματισμών που δημιουργείται από την αλληλεπίδραση ανάστροφων μικηλλίων με ένα υδατοδιαλυτό πολυμερές. Στη συνέχεια τα ανάστροφα μικήλλια χρησιμοποιήθηκαν σαν μέσο στο οποίο παρασκευάστηκαν νανονωματίδια τιτάνιας με υδρόλυση αλκοξειδίων και πολυμερισμό. Κατόπιν προχωρήσαμε στην παρασκευή και μελέτη λεπτών υμενίων από νανοσωματίδια τιτάνιας.
Preoperative spinopelvic hypermobility resolves following total hip arthroplasty
Authors: Sculco Peter K. , Windsor Eric N. , Jerabek Seth A. , Mayman David J. , Elbuluk Ameer , Buckland Aaron J. , Vigdorchik Jonathan M.
Published: December 2021

Aims

Spinopelvic mobility plays an important role in functional acetabular component position following total hip arthroplasty (THA). The primary aim of this study was to determine if spinopelvic hypermobility persists or resolves following THA. Our second aim was to identify patient demographic or radiological factors associated with hypermobility and resolution of hypermobility after THA.

Methods

This study investigated patients with preoperative posterior hypermobility, defined as a change in sacral slope (SS) from standing to sitting (ΔSSstand-sit) ≥ 30°. Radiological spinopelvic parameters, including SS, pelvic incidence (PI), lumbar lordosis (LL), PI-LL mismatch, anterior pelvic plane tilt (APPt), and spinopelvic tilt (SPT), were measured on preoperative imaging, and at six weeks and a minimum of one year postoperatively. The severity of bilateral hip osteoarthritis (OA) was graded using Kellgren-Lawrence criteria.

Results

A total of 136 patients were identified as having preoperative spinopelvic hypermobility. At one year after THA, 95% (129/136) of patients were no longer categorized as hypermobile on standing and sitting radiographs (ΔSSstand-sit < 30°). Mean ΔSSstand-sit decreased from 36.4° (SD 5.1°) at baseline to 21.4° (SD 6.6°) at one year (p < 0.001). Mean SSseated increased from baseline (11.4° (SD 8.8°)) to one year after THA by 11.5° (SD 7.4°) (p < 0.001), which correlates to an 8.5° (SD 5.5°) mean decrease in seated functional cup anteversion. Contralateral hip OA was the only radiological predictor of hypermobility persisting at one year after surgery. The overall reoperation rate was 1.5%.

Conclusion

Spinopelvic hypermobility was found to resolve in the majority (95%) of patients one year after THA. The increase in SSseated was clinically significant, suggesting that current target recommendations for the hypermobile patient (decreased anteversion and inclination) should be revisited. Cite this article: Bone Joint J 2021;103-B(12):1766–1773.
microRNA-136-5p from bone marrow mesenchymal stem cell-derived exosomes facilitates fracture healing by targeting LRP4 to activate the Wnt/β-catenin pathway
Authors: Yu Haichi , Zhang Jun , Liu Xiaoning , Li Yingzhi
Published: December 2021

Aims

Exosomes derived from bone marrow mesenchymal stem cells (BMSCs) have been reported to be a promising cellular therapeutic approach for various human diseases. The current study aimed to investigate the mechanism of BMSC-derived exosomes carrying microRNA (miR)-136-5p in fracture healing.

Methods

A mouse fracture model was initially established by surgical means. Exosomes were isolated from BMSCs from mice. The endocytosis of the mouse osteoblast MC3T3-E1 cell line was analyzed. CCK-8 and disodium phenyl phosphate microplate methods were employed to detect cell proliferation and alkaline phosphatase (ALP) activity, respectively. The binding of miR-136-5p to low-density lipoprotein receptor related protein 4 (LRP4) was analyzed by dual luciferase reporter gene assay. HE staining, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemistry were performed to evaluate the healing of the bone tissue ends, the positive number of osteoclasts, and the positive expression of β-catenin protein, respectively.

Results

miR-136-5p promoted fracture healing and osteoblast proliferation and differentiation. BMSC-derived exosomes exhibited an enriched miR-136-5p level, and were internalized by MC3T3-E1 cells. LRP4 was identified as a downstream target gene of miR-136-5p. Moreover, miR-136-5p or exosomes isolated from BMSCs (BMSC-Exos) containing miR-136-5p activated the Wnt/β-catenin pathway through the inhibition of LRP4 expression. Furthermore, BMSC-derived exosomes carrying miR-136-5p promoted osteoblast proliferation and differentiation, thereby promoting fracture healing.

Conclusion

BMSC-derived exosomes carrying miR-136-5p inhibited LRP4 and activated the Wnt/β-catenin pathway, thus facilitating fracture healing. Cite this article: Bone Joint Res 2021;10(12):744–758.