bluuebunny/crossref_metadata_2025 huggingface dataset:
A Holistic Approach for the Identification of Success Factors in Secondary Cleft Osteoplasty
Authors: Schröder Tom A. , Maiwald Martin , Reinicke Axel , Teicher Uwe , Seidel André , Schmidt Thorsten , Ihlenfeldt Steffen , Kozak Karol , Pradel Winnie , Lauer Günter , Ben Achour Anas
Published: March 2022

Cleft lip and palate belong to the most frequent craniofacial anomalies. Secondary osteoplasty is usually performed between 7 and 11 years with the closure of the osseus defect by autologous bone. Due to widespread occurrence of the defect in conjunction with its social significance due to possible esthetic impairments, the outcome of treatment is of substantial interest. The success of the treatment is determined by the precise rebuilding of the dental arch using autologous bone from the iliac crest. A detailed analysis of retrospective data disclosed a lack of essential and structured information to identify success factors for fast regeneration and specify the treatment. Moreover, according to the current status, no comparable process monitoring is possible during osteoplasty due to the lack of sensory systems. Therefore, a holistic approach was developed to determine the parameters for a successful treatment via the incorporation of patient data, the treatment sequences and sensor data gained by an attachable sensor module into a developed Dental Tech Space (DTS). This approach enables heterogeneous data sets to be linked inside of DTS, archiving and analysis, and is also for future considerations of respective patient-specific treatment plans.
Social Behavior Deficiencies in Captive American Alligators (Alligator mississippiensis)
Authors: Walsh Zane Cullinane , Olson Hannah , Clendening Miranda , Rycyk Athena
Published: March 2022

Understanding how the behavior of captive American alligator (Alligator mississippiensis) congregations compares to wild congregations is essential to assessing the welfare of alligators in captivity. Wild alligator congregations perform complex social behaviors, but it is unknown if such behaviors occur in captive congregations as frequently. We observed the behaviors of a captive and wild congregation of American alligators in Florida, USA in January 2021. Social behaviors were, on average, 827% more frequent in the wild congregation than the captive, and the wild congregation had a richer repertoire of social behaviors, with growling and HOTA (head oblique tail arched) behaviors being particularly common. High walking, a nonsocial behavior, dominated the behavioral repertoire of the captive congregation (94% of behaviors, excluding feeding) and may be a stereotypy that can be used as an indicator of welfare. Both congregations experienced human disturbance and displayed flushing as a species-specific defense reaction. Captive environments differ from the wild with respect to size, structure, stocking density, resource availability, and human presence. These differences translate into behavioral differences between wild and captive congregations. We identified important behavioral differences between wild and captive alligator congregations that can serve as a platform for more detailed investigations of alligator welfare in captivity.
Hemodialysis Therapy Adherence and Contributing Factors among End-Stage Renal Disease Patients at Muhimbili National Hospital, Dar es Salaam, Tanzania
Authors: Mohamedi Salumu , Mosha Idda Hubert
Published: March 2022

Maintenance hemodialysis services continue to rise globally. We evaluated adherence to hemodialysis therapy and contributing factors among end-stage renal disease patients at Muhimbili National Hospital, Dar es Salaam, Tanzania. A cross-sectional study was done involving 265 end-stage renal disease patients who were being treated at Muhimbili National Hospital, in Dar es Salaam. The study population selected included adult patients (18 years and above) with end-stage renal disease (ESRD) who were on hemodialysis. Data were entered into SPSS and imported into STATA for analysis. Numeric variables were summarized using the mean and standard deviation, and categorical variables were summarized using frequencies and proportions. A log-binomial regression model was used to estimate the risk factors associated with non-adherence, while multivariable log-binomial regression was used to control for confounders and test for the effect modifiers. All tests were two-tailed and the significance level was set at 5%. The parameters used to assess non-adherence to hemodialysis therapy in this study were the skipping of one or more hemodialysis sessions within the most recent 1 month or shortening one or more hemodialysis sessions by 10 or more minutes within the most recent 1 month. A session missed due to hospitalization or other medical reasons was not considered as non-adherence. All 265 patients were included in the analysis, of whom 191 (72.1%) were males. The rate of full adherence to the hemodialysis sessions was 64.2%. However, factors associated with the adherence to hemodialysis were the source of funding for hemodialysis (RR = 0.70, 95% CI: 0.48, 0.96), the occupation of the respondents (RR = 1.59, 95% CI: 1.44, 1.94), marital status (RR = 1.97, 95% CI: 1.03, 3.77), and monthly income (RR = 0.92, 95% CI: 0.62, 1.51). Adherence to hemodialysis therapy among ESRD patients at Muhimbili National Hospital was high and within the range of most published studies. Hemodialysis attendance among participants depended on the source of funding, marital status, occupation, and monthly income. Patients should be informed by the health care workers on the importance of adhering to hemodialysis sessions. Further studies should be done to explore the factors influencing adherence to hemodialysis, as it was noted that adherence was low among some patients.
SARS-CoV-2 Leads to Significantly More Severe Olfactory Loss than Other Seasonal Cold Viruses
Authors: Haehner Antje , Marquardt Belinda , Kardashi Romina , de With Katja , Rößler Susann , Landis Basile Nicolas , Welge-Luessen Antje , Hummel Thomas
Published: March 2022

The aim of this study was to investigate whether COVID-associated olfactory impairment differs from olfactory disorders due to other upper respiratory tract infections. We investigated the frequency of a SARS-CoV-2 infection among subjects presenting with a subjective olfactory impairment to a corona outpatient clinic between October 2020 and March 2021. Olfactory and gustatory loss were tested psychophysically, and the type of infection, SARS-CoV-2 versus 14 other common cold viruses, was assessed with nasopharyngeal swabs. Differences between the smell impairment caused by the pathogens were compared. Out of the 2120 patients, 314 reported sudden smell and/or taste loss (14%). In 68.9% of them, olfactory and in 25.6%, gustatory dysfunction could be confirmed by psychophysical testing. Of those with a psychophysically determined loss of smell, 61% were tested positive for SARS-CoV-2. SARS-CoV-2 led to a significantly more severe loss of smell and more qualitative olfactory disorders than other pathogens. Apart from rhinorrhea, shortness of breath and sore throat accompanying cold symptoms do not differ significantly between the viruses indicating the particular importance of smell loss in the differential diagnosis of seasonal colds. Multiplex-PCR in non-COVID patients revealed that only 27% of them had rhinoviruses, whereas the remainder were no further identified pathogens. Olfactory screening significantly increases diagnostic accuracy in COVID-19 patients compared to subjective assessment of olfactory loss.
LC-MS/MS-Based Serum Protein Profiling for Identification of Candidate Biomarkers in Pakistani Rheumatoid Arthritis Patients
Authors: Jahangir Sidrah , John Peter , Bhatti Attya , Aslam Muhammad Muaaz , Mehmood Malik Javaid , Anderson James R. , Peffers Mandy J.
Published: March 2022

Rheumatoid arthritis is an autoimmune disorder of complex disease etiology. Currently available serological diagnostic markers lack in terms of sensitivity and specificity and thus additional biomarkers are warranted for early disease diagnosis and management. We aimed to screen and compare serum proteome profiles of rheumatoid arthritis serotypes with healthy controls in the Pakistani population for identification of potential disease biomarkers. Serum samples from rheumatoid arthritis patients and healthy controls were enriched for low abundance proteins using ProteoMinerTM columns. Rheumatoid arthritis patients were assigned to one of the four serotypes based on anti-citrullinated peptide antibodies and rheumatoid factor. Serum protein profiles were analyzed via liquid chromatography-tandem mass spectrometry. The changes in the protein abundances were determined using label-free quantification software ProgenesisQITM followed by pathway analysis. Findings were validated in an independent cohort of patients and healthy controls using an enzyme-linked immunosorbent assay. A total of 213 proteins were identified. Comparative analysis of all groups (false discovery rate < 0.05, >2-fold change, and identified with ≥2 unique peptides) identified ten proteins that were differentially expressed between rheumatoid arthritis serotypes and healthy controls including pregnancy zone protein, selenoprotein P, C4b-binding protein beta chain, apolipoprotein M, N-acetylmuramoyl-L-alanine amidase, catalytic chain, oncoprotein-induced transcript 3 protein, Carboxypeptidase N subunit 2, Apolipoprotein C-I and Apolipoprotein C-III. Pathway analysis predicted inhibition of liver X receptor/retinoid X receptor activation pathway and production of nitric oxide and reactive oxygen species pathway in macrophages in all serotypes. A catalogue of potential serum biomarkers for rheumatoid arthritis were identified. These biomarkers can be further evaluated in larger cohorts from different populations for their diagnostic and prognostic potential.
Development of Water-Soluble Type I Photoinitiators for Hydrogel Synthesis
Authors: Aubry Bérengère , Dumur Frédéric , Lansalot Muriel , Bourgeat-Lami Elodie , Lacôte Emmanuel , Lalevée Jacques
Published: March 2022

In this work, two new water-soluble photoinitiators based on the α-alkoxy-arylketone scaffold have been synthesized and investigated for their ability to initiate photopolymerization for the preparation of hydrogels. The efficiency of these new Type I photoinitiators was compared to that of benchmark ones (2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone—Irgacure 2959 and 2-hydroxy-2-methyl propiophenone—Irgacure 1173). In combination with additive (carbene-borane), a good initiating ability was found under air. Mechanical properties of the prepared hydrogels were investigated by tensile tests and dynamic mechanical analysis (DMA). Markedly, hydrogels could be prepared with the newly proposed initiating systems in mild conditions (i.e., under air, using low light intensity @405 or 395 nm and without specialized glassware) and exhibited similar properties to those prepared by harsher approaches (thermal treatment or UV light).
Adaptive Evolutionary Computation for Nonlinear Hammerstein Control Autoregressive Systems with Key Term Separation Principle
Authors: Altaf Faisal , Chang Ching-Lung , Chaudhary Naveed Ishtiaq , Raja Muhammad Asif Zahoor , Cheema Khalid Mehmood , Shu Chi-Min , Milyani Ahmad H.
Published: March 2022

The knacks of evolutionary and swarm computing paradigms have been exploited to solve complex engineering and applied science problems, including parameter estimation for nonlinear systems. The population-based computational heuristics applied for parameter identification of nonlinear systems estimate the redundant parameters due to an overparameterization problem. The aim of this study was to exploit the key term separation (KTS) principle-based identification model with adaptive evolutionary computing to overcome the overparameterization issue. The parameter estimation of Hammerstein control autoregressive (HC-AR) systems was conducted through integration of the KTS idea with the global optimization efficacy of genetic algorithms (GAs). The proposed approach effectively estimated the actual parameters of the HC-AR system for noiseless as well as noisy scenarios. The simulation results verified the accuracy, convergence, and robustness of the proposed scheme. While consistent accuracy and reliability of the designed approach was validated through statistical assessments on multiple independent trials.
Diagnostic Criteria for Metabolic Syndrome in High-Altitude Regions: A Systematic Review
Authors: Villegas-Abrill Claudia Beatriz , Vidal-Espinoza Rubén , Gomez-Campos Rossana , Ibañez-Quispe Vladimiro , Mendoza-Mollocondo Charles , Cuentas-Yupanqui Sara Ruth , Fuentes-López José , Urra-Albornoz Camilo , Cossio-Bolaños Marco
Published: March 2022

Background and Objectives: Metabolic syndrome (MS) has many risk factors that are important to investigate in populations living at sea level and in high-altitude geographic regions. The aim was to identify the components of MS that cross-sectional studies use to assess in adult populations residing in high-altitude regions. Materials and Methods: A systematic review study was conducted. The Pubmed database was used. The search for original articles (cross-sectional) was performed from January 2013 to December 2020. The procedure was carried out by two researchers. The keywords used were metabolic syndrome, adults, and altitude regions. The search strategy considered the components of the PICOS tool. Results: Ten cross-sectional studies were identified in the Pubmed database from 2014 to 2020. Altitude levels varied between countries and regions, from 2060 to 4900 m above sea level. Three studies were conducted in both China and Peru, two studies in Ecuador, and one in Bolivia and India. The age ranges studied were from 18 to ~80 years of age, approximately. The components used to assess MS in most studies (between 9 to 10 studies) were body mass index (BMI), waist circumference (WC), blood pressure (BP), triglycerides (TG), high-density lipoproteins (HDL) and serum glucose (SG). Conclusions: This systematic review verified that the most commonly used domains in adult populations in various moderate- and high-altitude regions of the world are BMI, WC, BP, TG, HDL, and SG. These results suggest that in order to evaluate and/or investigate MS in subjects residing in high-altitude populations, at least four diagnostic domains should be considered in their protocols.
The Short and Intensive Rehabilitation (SHAiR) Program Improves Dropped Head Syndrome Caused by Amyotrophic Lateral Sclerosis: A Case Report
Authors: Urata Ryunosuke , Igawa Tatsuya , Suzuki Akifumi , Sasao Yutaka , Isogai Norihiro , Funao Haruki , Ishii Ken
Published: March 2022

Background and Objectives: Dropped head syndrome (DHS) is a syndrome that presents with correctable cervical kyphotic deformity as a result of weakening cervical paraspinal muscles. DHS with amyotrophic lateral sclerosis (ALS) is a relatively rare condition, and there is no established treatment. This is the first case report describing the improvement of both dropped head (DH) and cervical pain after the short and intensive rehabilitation (SHAiR) program in an ALS patient with DHS. Case Report: After being diagnosed with ALS in June 2020, a 75-year-old man visited our hospital in October 2020 to receive treatment for DHS. At the initial visit, the patient’s DH was prominent during standing and walking. The pain intensity of the neck was 9 out of 10 on the numerical rating scale (NRS), which was indicative of severe pain. The patient was hospitalized for 2 weeks and admitted into the SHAiR program. DH began to decrease one week after undergoing the SHAiR program and improved two weeks later. Neck pain decreased from 9 to 6 on the NRS. Results: The SHAiR program is a rehabilitation program aimed at improving DH in patients with idiopathic DHS. The program was designed to improve neck extensor and flexor function and global spinal alignment, and the program may have contributed to the improvement of DH and neck pain. Currently, reports of conservative therapies for this disease are limited to the use of cervical orthosis. Although further research is needed on the safety and indications of treatment, the SHAiR program may be a viable treatment option.
Current Updates on Expanded Carrier Screening: New Insights in the Omics Era
Authors: Veneruso Iolanda , Di Resta Chiara , Tomaiuolo Rossella , D’Argenio Valeria
Published: March 2022

Genetic carrier screening has been successfully used over the last decades to identify individuals at risk of transmitting specific DNA variants to their newborns, thus having an affected child. Traditional testing has been offered based on familial and/or ethnic backgrounds. The development of high-throughput technologies, such as next-generations sequencing, able to allow the study of large genomic regions in a time and cost-affordable way, has moved carrier screening toward a more comprehensive and extensive approach, i.e., expanded carrier screening (ECS). ECS simultaneously analyses several disease-related genes and better estimates individuals’ carrier status. Indeed, it is not influenced by ethnicity and is not limited to a subset of mutations that may arise from poor information in some populations. Moreover, if couples carry out ECS before conceiving a baby, it allows them to obtain a complete estimation of their genetic risk and the possibility to make an informed decision regarding their reproductive life. Despite these advantages, some weakness still exists regarding, for example, the number of genes and the kind of diseases to be analyzed and the interpretation and communication of the obtained results. Once these points are fixed, it is expectable that ECS will become an ever more frequent practice in clinical settings.
Three-Dimensional Morphology and Analysis of Widmanstätten Sideplates Ferrite
Authors: Cao Shengli , Wu Shaowen , Zhang Caijun , Zhang Qingjun
Published: March 2022

The three-dimensional (3D) morphology and crystal structure of Widmanstätten sideplate ferrite were simulated using a focused ion beam (FIB) scanning electron microscope equipped with electron backscatter diffraction (EBSD). The primary Widmanstätten sideplates nucleated and grew directly at the austenite grain boundary (GB). A certain included angle between the sideplates and the austenite GB was observed. The sideplates grew approximately parallel to the grain, and were separated by a small-angle GB. The primary Widmanstätten sideplates are best described as “∃” shaped, with a long intermediate ferrite strip. The interface with the austenite GB was smooth and flat, and the sideplate surface contained pits and holes. The secondary Widmanstätten sideplates nucleated and grew on the surface of the proeutectoid GB ferrite, with the sideplates and GB ferrite perpendicular to each other. Sideplates parallel to one another grew into the grain, and were separated by small-angle GB. The 3D morphology was distinguished by its “comb” shape. The sideplates’ tail was clustered and its front end remained sharp. The contact side of the GB ferrite was smooth and flat. The surface contained several uneven pits and defects.
Research on High-Temperature Compressive Properties of Ti–10V–1Fe–3Al Alloy
Authors: Li Cong , Huang Can , Ding Zhili , Zhou Xing
Published: March 2022

To investigate the thermal deformation behavior of Ti–10V–2Cr–3Al titanium alloy, the hot compression experiments were carried out via a strain rate of 0.1–0.001 s−1 and deformation temperature of 730~880 °C. The results showed that the rheological stress decreases when the deformation temperature increases or strain rate decreases. Due to the deformation conditions, some flow curves exhibited significant discontinuous yielding and flow softening. Flow softening in the α+β phase region was dominated by dynamic recrystallization (DRX), while in the β phase region, it was centered on dynamic recovery (DRV). A high-temperature constitutive equation, with good predictive power, was established.
Effect of Milling Parameters on Mechanical Properties and In Vitro Biocompatibility of Mg-Zn-Co Ternary Alloy
Authors: Mukhtar Sehrish , Kamran Muhammad , Ahmed Rafiq , Tayyeb Asima
Published: March 2022

Magnesium (Mg) is a potential candidate for biomedical implants, but its susceptibility to suffer corrosion attack in human body fluid limits its practical use. Thus, alloying Mg with other metal elements is the most effective strategy to improve its mechanical properties and biocompatibility. Herein, we report a Mg-Zn-Co ternary alloy (85-10-5 wt %) synthesized by the mechanical alloying technique. Ball-milling parameters such as ball size and milling time were varied to obtain better alloy properties. After compaction and sintering, the obtained alloy samples were subjected to various characterizations, including grain, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy, X-ray diffraction (XRD), microhardness and nanoindentation analyses. In vitro biocompatibility analysis of different alloys was also performed with MC3T3-E1 osteoblasts. Grain analysis confirmed the even dispersion of particles, while SEM analysis showed the formation of laminates, spherical and fine particles with an increase in time and varied ball size. XRD results further confirmed the formation of intermetallic compounds. The microhardness of samples was increased with the increase in milling time. The Young’s modulus of ternary alloys obtained from nanoindentation analysis was comparable to the modulus of human bone. Moreover, in vitro analysis with osteoblasts showed that the developed alloys were noncytotoxic and biocompatible.
Bioaccumulation of Blood Long-Chain Fatty Acids during Hemodialysis
Authors: Liu Tong , Dogan Inci , Rothe Michael , Reichardt Jana , Knauf Felix , Gollasch Maik , Luft Friedrich C. , Gollasch Benjamin
Published: March 2022

Long-chain fatty acids (LCFAs) serve as energy sources, components of cell membranes, and precursors for signaling molecules. Uremia alters LCFA metabolism so that the risk of cardiovascular events in chronic kidney disease (CKD) is increased. End-stage renal disease (ESRD) patients undergoing dialysis are particularly affected and their hemodialysis (HD) treatment could influence blood LCFA bioaccumulation and transformation. We investigated blood LCFA in HD patients and studied LCFA profiles in vivo by analyzing arterio–venous (A–V) LFCA differences in upper limbs. We collected arterial and venous blood samples from 12 ESRD patients, before and after HD, and analyzed total LCFA levels in red blood cells (RBCs) and plasma by LC–MS/MS tandem mass spectrometry. We observed that differences in arterial and venous LFCA contents within RBCs (RBC LCFA A–V differences) were affected by HD treatment. Numerous saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) n-6 showed negative A–V differences, accumulated during peripheral tissue perfusion of the upper limbs, in RBCs before HD. HD reduced these differences. The omega-3 quotient in the erythrocyte membranes was not affected by HD in either arterial or venous blood. Our data demonstrate that A–V differences in fatty acids status of LCFA are present and active in mature erythrocytes and their bioaccumulation is sensitive to single HD treatment.
A Comparative and Comprehensive Characterization of Polyphenols of Selected Fruits from the Rosaceae Family
Authors: Hameed Ahsan , Liu Ziyao , Wu Hanjing , Zhong Biming , Ciborowski Michal , Suleria Hafiz Ansar Rasul
Published: March 2022

The present research presents a comprehensive characterization of polyphenols from peach, pear, and plum using liquid chromatography coupled with electrospray ionization quadrupole-time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS), followed by the determination of their antioxidant potential. Plums showed the highest total phenolic content (TPC; 0.62 mg GAE/g), while peaches showed the highest total flavonoid content (TFC; 0.29 mg QE/g), also corresponding to their high scavenging activities (i.e., DPPH, ABTS, FRAP, and TAC). In all three fruit samples, a total of 51 polyphenolic compounds were tentatively identified and were mainly characterized from hydroxybenzoic acids, hydroxycinnamic acids, hydroxyphenylpentanoic acids, flavanols, flavonols, and isoflavonoids subclasses. Twenty targeted phenolic compounds were quantified using high-performance liquid chromatography with photodiode array detection (HPLC-PDA). The plum cultivar showed the highest content of phenolic acids (chlorogenic acid, 11.86 mg/100 g), whereas peach samples showed the highest concentration of flavonoids (catechin, 7.31 mg/100 g), as compared to pear. Based on these findings, the present research contributes and complements the current characterization data of these fruits presented in the literature, as well as ensures and encourages the utilization of these fruits in different food, feed, and nutraceutical industries.
Atomic Model of Gold Adsorption onto the Pyrite Surface with DFT Study
Authors: Liu Chunlin , Li Yongbing , Cheng Qi , Zhao Yang
Published: March 2022

Adsorption mineralization of gold is an important mineralization mechanism under epigenetic and low temperature conditions. In this paper, a plane-wave pseudopotential method based on density functional theory (DFT) is used to explore the adsorption mechanism of gold on the surface of pyrite. Among the three surfaces of pyrite, the surface energies of (100), (111), and (210) surfaces are 1.0508, 1.5337, and 1.8255 J∙m2, respectively, and the (100) surface is the most stable surface in the thermodynamic state. The adsorption capacities of gold atoms under different surfaces are (210) (−2.68 eV) > (111) (−1.67 eV) > (100) (−0.84 eV). Mulliken analysis indicates that charge transfer occurs after the adsorption of gold atoms onto the surface of pyrite (210), and gold and iron atoms are oxidized with the reduction of sulfur atoms. The density of states (PDOS) analysis shows that the 5d orbital on the Fermi energy level of the iron atom is active and the adsorption capacity is greater than that of the sulfur atom, and adsorption is formed between the gold atom, which leads to the gold being able to be stably deposited on the surface of pyrite (210).
Bioprospecting the Antibiofilm and Antimicrobial Activity of Soil and Insect Gut Bacteria
Authors: Raffaelli Sofía , Abreo Eduardo , Altier Nora , Vázquez Álvaro , Alborés Silvana
Published: March 2022

Antimicrobial resistance is a growing concern in public health and current research shows an important role for bacterial biofilms in recurrent or chronic infections. New strategies, therefore, are necessary to overcome antimicrobial resistance, through the development of new therapies that could alter or inhibit biofilm formation. In this sense, antibiofilm natural products are very promising. In this work, a bioprospection of antimicrobial and antibiofilm extracts from Uruguayan soil bacteria and insect gut bacteria was carried out. Extracts from extracellular broths were tested for their ability to inhibit planktonic cell growth and biofilm formation. Genomic analysis of Bacillus cereus ILBB55 was carried out. All extracts were able to inhibit the growth of, at least, one microorganism and several extracts showed MICs lower than 500 µg mL−1 against microorganisms of clinical relevance (Staphylococcus aureus, Pseudomonas aeruginosa, and Enterobacter cloacae). Among the extracts evaluated for biofilm inhibition only ILBB55, from B. cereus, was able to inhibit, S. aureus (99%) and P. aeruginosa (62%) biofilms. Genomic analysis of this strain showed gene clusters similar to other clusters that code for known antimicrobial compounds. Our study revealed that extracts from soil bacteria and insect gut bacteria, especially from B. cereus ILBB55, could be potential candidates for drug discovery to treat infectious diseases and inhibit S. aureus and P. aeruginosa biofilms.
Flavanone Glycosides, Triterpenes, Volatile Compounds and Antimicrobial Activity of Miconia minutiflora (Bonpl.) DC. (Melastomataceae)
Authors: Ferreira Nathália Siso , Cascaes Márcia Moraes , da Silva Santos Lourivaldo , de Oliveira Mozaniel Santana , das Graças Bichara Zoghbi Maria , Araújo Isabella Santos , Uetanabaro Ana Paula Trovatti , de Aguiar Andrade Eloisa Helena , Guilhon Giselle Maria Skelding Pinheiro
Published: March 2022

Chemical composition of the essential oils and extracts and the antimicrobial activity of Miconia minutiflora were investigated. The flavanone glycosides, pinocembroside and pinocembrin-7-O-[4″,6″-HHDP]-β-D-glucose, were identified, along with other compounds that belong mainly to the triterpene class, besides the phenolics, gallic acid and methyl gallate. Sesquiterpenes and monoterpenes were the major compounds identified from the essential oils. Screening for antimicrobial activity from the methanolic extract of the leaves showed that the MIC and MMC values against the tested microorganisms ranged from 0.625 to 5 mg·mL−1 and that the extract was active against microorganisms, Staphyloccocus aureus, Escherichia coli, and Bacillus cereus.
Synthesis of Silver Nanoparticles from Extracts of Wild Ginger (Zingiber zerumbet) with Antibacterial Activity against Selective Multidrug Resistant Oral Bacteria
Authors: Ramzan Muhammad , Karobari Mohmed Isaqali , Heboyan Artak , Mohamed Roshan Noor , Mustafa Mohammed , Basheer Syed Nahid , Desai Vijay , Batool Salma , Ahmed Naveed , Zeshan Basit
Published: March 2022

Antibiotic resistance rate is rising worldwide. Silver nanoparticles (AgNPs) are potent for fighting antimicrobial resistance (AMR), independently or synergistically. The purpose of this study was to prepare AgNPs using wild ginger extracts and to evaluate the antibacterial efficacy of these AgNPs against multidrug-resistant (MDR) Staphylococcus aureus, Streptococcus mutans, and Enterococcus faecalis. AgNPs were synthesized using wild ginger extracts at room temperature through different parameters for optimization, i.e., pH and variable molar concentration. Synthesis of AgNPs was confirmed by UV/visible spectroscopy and further characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy analysis (EDXA), and Fourier-transform infrared spectroscopy (FTIR). Disc and agar well diffusion techniques were utilized to determine the in vitro antibacterial activity of plant extracts and AgNPs. The surface plasmon resonance peaks in absorption spectra for silver suspension showed the absorption maxima in the range of 400–420 nm. Functional biomolecules such as N–H, C–H, O–H, C–O, and C–O–C were present in Zingiber zerumbet (Z. zerumbet) (aqueous and organic extracts) responsible for the AgNP formation characterized by FTIR. The crystalline structure of ZZAE-AgCl-NPs and ZZEE-AgCl-NPs was displayed in the XRD analysis. SEM analysis revealed the surface morphology. The EDXA analysis also confirmed the element of silver. It was revealed that AgNPs were seemingly spherical in morphology. The biosynthesized AgNPs exhibited complete antibacterial activity against the tested MDR bacterial strains. This study indicates that AgNPs of wild ginger extracts exhibit potent antibacterial activity against MDR bacterial strains.
Plants Used in Mexican Traditional Medicine for the Management of Urolithiasis: A Review of Preclinical Evidence, Bioactive Compounds, and Molecular Mechanisms
Authors: Sansores-España Delia , Pech-Aguilar Alfredo Geovanny , Cua-Pech Karol Guadalupe , Medina-Vera Isabel , Guevara-Cruz Martha , Gutiérrez-Solis Ana Ligia , Reyes-García Juan G. , Avila-Nava Azalia
Published: March 2022

Urolithiasis (UL) involves the formation of stones in different parts of the urinary tract. UL is a health problem, and its prevalence has increased considerably in developing countries. Several regions use plants in traditional medicine as an alternative in the treatment or prevention of UL. Mexico has known about the role of traditional medicine in the management of urinary stones. Mexican traditional medicine uses plants such as Argemone mexicana L., Berberis trifoliata Hartw. ex Lindl., Costus mexicanus Liebm, Chenopodium album L., Ammi visnaga (L.) Lam., Eysenhardtia polystachya (Ortega) Sarg., Selaginella lepidophylla (Hook. & Grev.) Spring, and Taraxacum officinale L. These plants contain different bioactive compounds, including polyphenols, flavonoids, phytosterols, saponins, furanochromones, alkaloids, and terpenoids, which could be effective in preventing the process of stone formation. Evidence suggests that their beneficial effects might be associated with litholytic, antispasmodic, and diuretic activities, as well as an inhibitory effect on crystallization, nucleation, and aggregation of crystals. The molecular mechanisms involving these effects could be related to antioxidant, anti-inflammatory, and antimicrobial properties. Thus, the review aims to summarize the preclinical evidence, bioactive compounds, and molecular mechanisms of the plants used in Mexican traditional medicine for the management of UL.
Quantitative Analysis of Daporinad (FK866) and Its In Vitro and In Vivo Metabolite Identification Using Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry
Authors: Park Minjae , Lee Byeong Ill , Choi Jangmi , Park Yuri , Park Seo-Jin , Lim Jeong-Hyeon , Lee Jiyu , Shin Young G.
Published: March 2022

Daporinad (FK866) is one of the highly specific inhibitors of nicotinamide phosphoribosyl transferase (NAMPT) and known to have its unique mechanism of action that induces the tumor cell apoptosis. In this study, a simple and sensitive liquid chromatography–quadrupole-time-of-flight–mass spectrometric (LC-qTOF-MS) assay has been developed for the evaluation of drug metabolism and pharmacokinetics (DMPK) properties of Daporinad in mice. A simple protein precipitation method using acetonitrile (ACN) was used for the sample preparation and the pre-treated samples were separated by a C18 column. The calibration curve was evaluated in the range of 1.02~2220 ng/mL and the quadratic regression (weighted 1/concentration2) was used for the best fit of the curve with a correlation coefficient ≥ 0.99. The qualification run met the acceptance criteria of ±25% accuracy and precision values for QC samples. The dilution integrity was verified for 5, 10 and 30-fold dilution and the accuracy and precision of the dilution QC samples were also satisfactory within ±25% of the nominal values. The stability results indicated that Daporinad was stable for the following conditions: short-term (4 h), long-term (2 weeks), freeze/thaw (three cycles). This qualified method was successfully applied to intravenous (IV) pharmacokinetic (PK) studies of Daporinad in mice at doses of 5, 10 and 30 mg/kg. As a result, it showed a linear PK tendency in the dose range from 5 to 10 mg/kg, but a non-linear PK tendency in the dose of 30 mg/kg. In addition, in vitro and in vivo metabolite identification (Met ID) studies were conducted to understand the PK properties of Daporinad and the results showed that a total of 25 metabolites were identified as ten different types of metabolism in our experimental conditions. In conclusion, the LC-qTOF-MS assay was successfully developed for the quantification of Daporinad in mouse plasma as well as for its in vitro and in vivo metabolite identification.
Hispidulin Ameliorates Endotoxin-Induced Acute Kidney Injury in Mice
Authors: Kim Kiryeong , Leem Jaechan
Published: March 2022

Lipopolysaccharide (LPS) is an endotoxin that plays a crucial role in septic acute kidney injury (AKI). Hispidulin is a natural flavonoid that possesses various biological activities. Recent studies have shown that hispidulin administration alleviates various inflammatory diseases in animal models. This study aimed to investigate the renoprotective effect of hispidulin on LPS-induced AKI. Male C57BL/6 mice were administered LPS (10 mg/kg) with or without hispidulin (50 mg/kg). Hispidulin administration attenuated renal dysfunction, histological alterations, and the upregulation of neutrophil gelatinase-associated lipocalin. This flavonoid also reduced cytokine production and Toll-like receptor 4 expression, inhibited nuclear factor-κB and mitogen-activated protein kinase cascades, and alleviated immune cell infiltration. The oxidation of lipids and DNA was also inhibited by hispidulin administration. This antioxidant effect of hispidulin was associated with the downregulation of NADPH oxidase 4, the activation of catalase and superoxide dismutase activities, and the restoration of glutathione levels. Moreover, hispidulin administration attenuated tubular cell apoptosis by inhibiting caspase-3 pathway. These data suggest that hispidulin ameliorates endotoxin-induced kidney injury by suppressing inflammation, oxidative stress, and tubular cell death.
The Transcriptomic Mechanism of a Novel Autolysis Induced by a Recombinant Antibacterial Peptide from Chicken Expressed in Pichia pastoris
Authors: Wang Dongsheng , Yu Xinjun , Sheng Ping , Zhang Guohua
Published: March 2022

Autolysis is a common physiological process in eukaryotic cells that is often prevented or applied, especially in yeast expression systems. In this study, an antimicrobial peptide from chicken (AMP) was recombinantly expressed in the Pichia pastoris expression system, which induced a series of cellular autolysis phenotypes after methanol treatment, such as the aggregated, lysed, irregular, and enlarged cell morphology, while the cells expressing a recombinant aflatoxin-detoxifizyme (ADTZ) were not autolyzed. A comparative transcriptomic analysis showed that the transcriptomic profiles of cells derived from the autolysis and non-autolysis groups were well discriminated, suggesting that the mechanisms of autolysis were at the transcriptional level. A further differential expression gene (DEG) analysis showed that the DEGs from the two groups were involved mainly in autophagy, the MAPK signaling pathway, transcriptional factors, the central carbon metabolism, anti-stress functions, and so on. In the autolysis group, the cell activity was significantly reduced with the MAPK signaling pathway, the central carbon metabolism was down-regulated, and components of the cytoplasm-to-vacuole targeting (CVT) and mitophagy pathways were up-regulated, suggesting that the autophagy involved in the trafficking of intracellular molecules in the vacuole and mitochondrion contributed to autolysis, which was regulated by transcriptional factors and signal pathways at the transcriptional level. This study provides a theoretical basis for genetic modifications to prevent or utilize cell autolysis in the recombinant protein expression system.
Removal of Acidic Organic Ionic Dyes from Water by Electrospinning a Polyacrylonitrile Composite MIL101(Fe)-NH2 Nanofiber Membrane
Authors: Jia Jiao , Wu Hao , Xu Le , Dong Fengchun , Jia Yongtang , Liu Xi
Published: March 2022

A nanofiber metal–organic framework filter, a polyacrylonitrile (PAN) nanofiber membrane composite with an iron/2-amino-terephthalic acid-based metal–organic framework (MIL101(Fe)-NH2), was prepared by one-step electrospinning. MIL101(Fe)-NH2 was combined into the polymer nanofibers in situ. PAN-MIL101(Fe)-NH2 composite nanofiber membranes (NFMs) were prepared from a homogeneous spinning stock containing MIL101(Fe)-NH2 prebody fluid and PAN. Crystallization of MIL101(Fe)-NH2 and solidification of the polymer occurred simultaneously during electrospinning. The PAN-MIL101(Fe)-NH2 composite NFM showed that MIL101(Fe)-NH2 was uniformly distributed throughout the nanofiber and was used to adsorb and separate acidic organic ionic dyes from the aqueous solution. The results of Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction analysis showed that MIL101(Fe)-NH2 crystals were effectively bonded in the PAN nanofiber matrix, and the crystallinity of MIL101(Fe)-NH2 crystals remained good, while the distribution was uniform. Owing to the synergistic effect of PAN and the MIL101(Fe)-NH2 crystal, the PAN-MIL101(Fe)-NH2 composite NFM showed a fast adsorption rate for acidic ionic dyes. This study provides a reference for the rapid separation and purification of organic ionic dyes from wastewater.
Borophene and Pristine Graphene 2D Sheets as Potential Surfaces for the Adsorption of Electron-Rich and Electron-Deficient π-Systems: A Comparative DFT Study
Authors: Ibrahim Mahmoud A. A. , Mahmoud Amna H. M. , Soliman Kamal A. , Mekhemer Gamal A. H. , Ahmed Muhammad Naeem , Shawky Ahmed M. , Abourehab Mohammed A. S. , Elkaeed Eslam B. , Soliman Mahmoud E. S. , Moussa Nayra A. M.
Published: March 2022

The versatility of striped borophene (sB), β12 borophene (β12), and pristine graphene (GN) to adsorb π-systems was comparatively assessed using benzene (BNZ) and hexafluorobenzene (HFB) as electron-rich and electron-deficient aromatic π-systems, respectively. Using the density functional theory (DFT) method, the adsorption process of the π-systems on the investigated 2D sheets in the parallel configuration was observed to have proceeded more favorably than those in the vertical configuration. According to the observations of the Bader charge transfer analysis, the π-system∙∙∙sB complexes were generally recorded with the largest contributions of charge transfer, followed by the π-system∙∙∙β12 and ∙∙∙GN complexes. The band structures of the pure sheets signaled the metallic and semiconductor characters of the sB/β12 and GN surfaces, respectively. In the parallel configuration, the adsorption of both BNZ and HFB showed more valence and conduction bands compared to the adsorption in the vertical configuration, revealing the prominent preferentiality of the anterior configuration. The density-of-states (DOSs) results also affirmed that the adsorption process of the BNZ and HFB on the surface of the investigated 2D sheets increased their electrical properties. In all instances, the sB and β12 surfaces demonstrated higher adsorptivity towards the BNZ and HFB than the GN analog. The findings of this work could make a significant contribution to the deep understanding of the adsorption behavior of aromatic π-systems toward 2D nanomaterials, leading, in turn, to their development of a wide range of applications.
Understanding Fish Larvae’s Feeding Biology to Improve Aquaculture Feeding Protocols
Authors: Ribeiro Laura , Hubert François Noel , Rodrigues Vera , Rojas-Garcia Carlos , Dinis Maria Teresa
Published: March 2022

Knowing that food ingestion and digestion are processes under neuroendocrine regulation, it is important to understand how fish larvae regulate these processes, when the digestive system itself is under development. This study aimed to analyze how gilthead seabream (Sparus aurata) larval feeding incidence and intensity were affected by time, light, and water temperature, through the analysis of gut content and how this affected cholecystokinin (CCK) content. Three short-term experiments were done: (A) 27 and 42 days post-hatch (DPH) larvae were analyzed at different intervals for 80 min, after feeding; (B) 38 and 48 DPH larvae were kept under different light intensities (0, 400, 1000 lux) for 30 min; (C) 41 DPH larvae were kept at 17, 19, 25 °C for 30 min. Feeding incidence below 100% was observed for 27 DPH larvae, and for 38 DPH larvae fed at 0 lux. Feeding intensity was several fold higher at older ages. However, the number of prey in the gut was lower at 0 lux, and higher at 25 °C. Overall, no clear pattern was observed for CCK, but at older ages increasing gut content resulted in higher CCK content. In conclusion, until 38 DPH light is important for gilthead sea bream larval feeding ability, and maturing functionality of CCK was only observed at later stages.
Modeling for Generating Femtosecond Pulses in an Er-Doped Fiber Using Externally Controlled Spectral Broadening and Compression Mechanisms
Authors: Abo-elenein Mohamed Hemdan , Hassab Elnaby Salah Eldeen Ibrahim , Hassan Amin Fahim , Abd-Rabou Afaf Mahmoud
Published: March 2022

A model for generating femtosecond laser pulses from a low-power mode-locked laser of moderate temporal half-width was proposed. This was achieved by injecting the pulse into a single-mode inverted-populated Er-doped fiber where self-focusing and absorption were avoided. To initiate spectral broadening, the pulse was phase-modulated by subjecting a part of the fiber to an electric field of suitable intensity and frequency generated into a circular capacitor. To stimulate temporal compression, the phase-modulated pulse was introduced into a combination of two prism sets located symmetrically with respect to the x-axis. After passing the pulse through the first prism set, its spectral components were spatially separated in the y-axis. The spectral phases were manipulated by redirecting the spectral components through a slab cross-section that was subjected to a spatially modulated DC electric field. After passing the slab, the pulse is directed into the second prism set, where the spectral components were spatially overlapped and propagated outside the compressor with the same slope and dimension as before entering the compressor. Constructive super positioning of the phase-manipulated spectral components gave maximum intensity only at a specified location.
Trends in the Structure of Nuclei near 100Sn
Authors: Górska Magdalena
Published: March 2022

Inevitable progress has been achieved in recent years regarding the available data on the structure of 100Sn and neighboring nuclei. Updated nuclear structure data in the region is presented using selected examples. State-of-the-art experimental techniques involving stable and radioactive beam facilities have enabled access to those exotic nuclei. The analysis of experimental data has established the shell structure and its evolution towards N = Z = 50 of the number of neutrons, N, and the atomic number, Z, seniority conservation and proton–neutron interaction in the g9/2 orbit, the super-allowed Gamow–Teller decay of 100Sn, masses and half-lives along the rapid neutron-capture process (r-process) path and super-allowed α decay beyond 100Sn. The status of theoretical approaches in shell model and mean-field investigations are discussed and their predictive power assessed. The calculated systematics of high-spin states for N = 50 isotopes including the 5− state and N = Z nuclei in the g9/2 orbit is presented for the first time.
“Contramodernist Buddhism” in a Global City-State: Shinnyo-en in Singapore
Authors: Phua Keng Yung
Published: March 2022

This article outlines the arrival and adaptation of Shinnyo-en as an example of contramodernist Buddhism in Singapore. Shinnyo-en’s contramodernist spirituality focuses on its founding Itō family. The arrival of Shinnyo-en is situated within the larger contexts of the Singapore–Japan relationship. Social memories of the Japanese occupation lingered within the population amidst increasing Japanese Foreign Domestic Investments in Singapore. These transnational migration trends brought Shinnyo-en practitioners and Shinnyo-en itself to Singapore. Simultaneously, Singapore’s government had been actively monitoring and regulating religious groups in order to maintain religious harmony, societal wellbeing, and ensure the separation of religion and politics in Singapore. This study explores the adaptations of Shinnyo-en’s organisational structure, religious practices, and activities in Singapore from 1983 to 2021. It argues that Shinnyo-en has actively adapted to the Singapore context and has actively courted the state for its political survival, adjusting its activities to gain social recognition from Singapore society as a Buddhist organisation. Despite these adaptations, Shinnyo-en Singapore retains its contramodernist Buddhist spirituality, focusing on its founding Itō family. This article highlights the integration of Shinnyo-en’s contramodernist beliefs within Shinnyo-en’s activities and how this contramodernist spirituality mobilises support for selected social causes through its practitioners.