bluuebunny/crossref_metadata_2025 huggingface dataset:
From Lab to Life: Exploring Cutting-Edge Models for Neurological and Psychiatric Disorders
Authors: Tanaka Masaru , Vécsei László
Published: March 2024

Neuroscience, neurology, and psychiatry are rapidly evolving fields that aim to understand the complex mechanisms underlying brain function and dysfunction, as well as to develop effective interventions for various neurological and psychiatric disorders [...]
MSR405: Inhibiting Neuroinflammation after Spinal Cord Injury in Rats
Authors: Liu Yu , Xiao Yu , Gao Jimeng , Gao Jiaxin , Li Ruicheng , Qi Zhongquan , Liu Xiaocun
Published: March 2024

The treatment of spinal cord injury (SCI) is often ineffective. Additionally, SCI-induced inflammation leads to secondary injury. Current anti-inflammatory hydrophilic drugs fail to reach the nerve injury site due to the blood–brain barrier. Here, we synthesized MSR405, a new lipophilic unsaturated fatty acid derivative of Radix Isatidis and investigated its therapeutic effect in SCI model rats. Furthermore, we systematically investigated its structure, toxicity, anti-inflammatory effect, and the underlying mechanism. MSR405 was injected into the abdominal cavity of the Sprague Dawley SCI model rats, and the effect on their behavioral scores and pathology was estimated to assess the status of neurological inflammation. Our data show that MSR405 treatment significantly improved the motor function of SCI rats, and markedly suppressed the associated neuroinflammation. Moreover, MSR405 could attenuate LPS-induced inflammatory response in BV2 cells (Mouse microglia cells) in vitro. Mechanistically, MSR405 inhibits proinflammatory cytokines, supporting the anti-inflammatory response. Additionally, MSR405 can significantly block the TLR4/NF-κB signaling pathway and nitric oxide production. In summary, MSR405 reduces inflammation in SCI rats through the TLR4/NF-κB signal cascade and can inhibit neuroinflammation after spinal cord injury.
Adapting to Changes in Communication: The Orbitofrontal Cortex in Language and Speech Processing
Authors: Jiang Xiaoming , Ma Xiquan , Sanford Ryan , Li Xun
Published: March 2024

Despite most studies on the neurobiology of language demonstrating the central part of the perisylvian network involved in language and speech function, this review attempts to complement this view by focusing on the role of the orbitofrontal cortex (OFC). This region is primarily involved in goal-directed adaptive behavior. Recently, there has been increasing evidence that the OFC is involved in language and speech tasks. This review demonstrates that not only the linguistic tasks that involve the processing of socially, pragmatically and emotionally relevant information engage OFC and its neurobiological mechanisms, but also specific receptive and expressive language performances rely on specific neurophysiological properties of this region (e.g., the gray matter volume and the functional activation of OFC and the uncinate fasciculus that connects OFC), which in many cases, demand executive functions. These findings highlight: (1) The OFC plays a relevant role in the adaptive neurobiological function of language; (2) the neurobiological mechanisms beyond linguistic and speech processes complement and interplay with the language-unique processes to achieve successful comprehension and production in the changing communicative contexts.
Dopamine Concentration Changes Associated with the Retrodialysis of Methylone and 3,4-Methylenedioxypyrovalerone (MDPV) into the Caudate Putamen
Authors: Goldsmith Robert , Aburahma Amal , Sprague Jon E.
Published: March 2024

Structural modifications to synthetic psychoactive cathinones (SPCs), a class of drugs that contain a β-keto modification of the phenethylamine pharmacophore of amphetamine, induce differences in dopamine transporter (DAT) activity. Here, in vivo retrodialysis was utilized to deliver the SPCs 3,4-methylenedioxypyrovalerone (MDPV, a DAT inhibitor) or methylone (a DAT substrate) into the caudate putamen of male Sprague-Dawley rats. Dialysate samples were collected prior to and post drug administration, and temporal changes in dopamine concentration were quantified using HPLC-EC methods. Methylone elicited a 200% increase and MDPV a 470% increase in dopamine levels at the 10 min time point. The findings demonstrate that in vivo retrodialysis can be used to evaluate the effects of SPCs on neurotransmission in the brain.
Between Laws and Trends: Unraveling the Dynamics of Vertical Housing Units’ Development under Institutional Forces in the Brazilian Amazon
Authors: Moreira Frederico Guilherme Pamplona , de Souza Alexander Hierro Ferreira , Bassalo José Maria Coelho , Farias Filho Milton Cordeiro
Published: March 2024

This paper is based on the recognition of a phenomenon occurring in the real estate market of Belém, Pará, in the Brazilian Amazon. The phenomenon refers to the relative increase in the size of the balconies of residential apartments launched in the city between 2005 and 2017 by construction companies, with the aim of increasing the number of units built per tower. Given that balconies were originally areas of low frequency for the occupants of apartments in this region, the aim of this article was to identify and analyze the institutional factors that support this product development strategy, which is becoming increasingly consolidated among companies. To investigate this phenomenon, we collected data from official documents provided by the Municipal Urban Planning Department (Secretaria Municipal de Urbanismo—SEURB), specifically focusing on the launch of residential high-rise buildings during the specified period. Regression techniques with ordinary least squares methods were applied, as well as econometric models of spatial autocorrelation. The results of the hypothesis tests confirmed the relationship between the restrictive parameters imposed by the city’s Urban Master Plan and the relative increase in apartment balconies. The spatial autocorrelation models confirmed the spatial spillover effect, showing that apartment projects with this characteristic tend to be concentrated in certain areas of the city, influencing each other. In order to discuss this phenomenon, the institutional theory was the protagonist of the analysis, showing how an organizational strategy can adapt to the authority that regulates the occupation of urban land in the city, meeting the two market forces and the demand and supply of apartments.
Hedonic Approach to Vertical Residential Rentals in the Brazilian Amazon: The Case of Belém, Pará
Authors: Freitas Yan Gabriel Pereira Magalhães de , Moreira Frederico Guilherme Pamplona , Souza Alexander Hierro Ferreira de , Santos Victor Igor Monteiro dos
Published: March 2024

This study set out to identify and analyze the factors that influence the formation of rental prices for residential apartments in the city of Belém. The approach adopted was based on the hedonic price theory, which considers that the rental price of an apartment reflects the implicit prices of its attributes—structural, locational, and neighborhood quality. The sample used consisted of 259 observations, corresponding to the rental advertisements of the representative apartments in each building. The ordinary least squares (OLS), spatial lag model (SLM), and geographically weighted regression (GWR) techniques were used in the statistical analysis in this study. The results of the OLS model showed statistical significance between the attributes analyzed and the rental price of the apartments. In turn, the SLM indicated that the structural attributes have an impact on the rental prices of neighboring apartments, configuring a contagion effect in the real estate market. The GWR model showed that there was no spatial heterogeneity in the effects of the determinants on apartment rental prices throughout the sample.
The Effect of Scrap Tires and Reclaimed Asphalt Pavement on the Behavior of Stone Columns
Authors: Bikdeli Hoora , Sharahi Morteza Jiryaei , Badarloo Baitollah , Lehner Petr
Published: March 2024

The objective of this investigation is to understand how to use waste tires to surround stone pillars and mix gravel with recycled asphalt pavement (RAP) and stone pillars to provide an environmentally friendly and cost-effective weak layer improvement method. To study the behavior of such stone columns, experiments were conducted in units consisting of a single stone column with recycled asphalt pavement as filling material and a single stone column covered with old tires. To test the effect of different mixing ratios, rapeseed content was selected from 0% to 100%. Elasticity tests were conducted on cladded and nonclad stone column samples. Furthermore, direct shear tests were conducted on samples with different ratios of gravel and rapeseed mixtures. The results of the load-bearing capacity test show that the cover of the stone columns with old tires can significantly increase the load-bearing capacity. Replacing 25% of natural stone column aggregates with RAP increases the load capacity. But as the percentage of RAP in the mixture increases from 25% to 100%, the loading capacity decreases. Another advantage is the reinforced stone column. From the point of view of ecology, an advantage is the use of recyclable materials.
Calibration of Equivalent Viscous Damping Expressions for Displacement-Based Design and Application to RC Frames
Authors: Landi Luca , Benfenati Cristiano , Quqa Said , Bernagozzi Giacomo , Diotallevi Pier Paolo
Published: March 2024

Equivalent viscous damping plays a central role in displacement-based design procedures. In this paper, approaches for estimating the equivalent viscous damping of RC frame buildings are proposed. At first, the analytical formulation of Blandon and Priestley was analysed, and then a calibration of the coefficients of this formulation was performed. Compared with the work of Blandon and Priestley, a larger set of synthetic accelerograms, related to different types of soil and different intensities, and a wider range of the effective periods were considered. In particular, two different sets of parameters are proposed: the first is usable in the case of spectra obtained numerically (approach 1), and the second is usable in the case of code-based spectra and damping modification factor (approach 2). To test the performed calibration and to compare the considered formulations (i.e., the proposed and literature equations), the direct displacement-based design procedure has been applied to three case studies of reinforced concrete frame structures, and then pushover and nonlinear time-history analyses have been performed. The results show that the use of the calibrated parameters (for both the considered approaches) has determined more conservative results, in terms of design base shear and maximum drift from NLTH. Moreover, the average displacement profiles and the inter-storey drifts obtained from time-history analyses for the frames designed with the calibrated parameters match better the design profile.
Let Us Get Regional: Exploring Prospects for Biomass-Based Carbon Dioxide Removal on the Ground
Authors: Otto Danny , Matzner Nils
Published: March 2024

In recent years, research on carbon dioxide removal (CDR) has significantly increased. Numerous studies have analyzed demonstration projects, outlined scenarios, modeled pathways, or focused on CDR’s national or international governance. However, regional case studies investigating the dynamics that may facilitate or impede the broader adoption of CDR methods in spatially explicit settings are critically absent. Understanding implementation contexts on the ground is vital, and comparing them across different removal methods is essential for effectively scaling up CDR. This paper aims to address this research gap by comparatively examining the development of biomass-based CDR in three regions of Germany. Taking an exploratory approach, we conducted surveys in these regions to gain insight into stakeholder perceptions of the following six CDR methods: forest management, agriculture and soil carbon, long-lasting building materials, rewetting of peatlands and paludiculture, biochar, and bioenergy with carbon capture and storage. In this article, we present the results of the stakeholder survey, which offers multiple perspectives that can shape future studies of regional implementation and yield policy-relevant guidance. Although our research primarily focuses on the regional level in Germany, it sheds light on various conflicts, uncertainties, and potentials that are likely to be relevant for the rollout of CDR in other countries. By examining these aspects, we contribute to the broader discourse on CDR and its potential implementation.
Venetoclax Resistance in Acute Myeloid Leukemia
Authors: Garciaz Sylvain , Hospital Marie-Anne , Collette Yves , Vey Norbert
Published: March 2024

Venetoclax is a BH3-mimetics agent interacting with the anti-apoptotic protein BCL2, facilitating cytochrome c release from mitochondria, subsequent caspases activation, and cell death. Venetoclax combined with azacitidine (VEN-AZA) has become a new standard treatment for AML patients unfit for intensive chemotherapy. In the phase III VIALE-A study, VEN-AZA showed a 65% overall response rate and 14.7 months overall survival in comparison with 22% and 8 months in the azacitidine monotherapy control arm. Despite these promising results, relapses and primary resistance to venetoclax are frequent and remain an unmet clinical need. Clinical and preclinical studies have been conducted to identify factors driving resistance. Among them, the most documented are molecular alterations including IDH, FLT3, TP53, and the newly described BAX mutations. Several non-genetic factors are also described such as metabolic plasticity, changes in anti-apoptotic protein expression, and dependencies, as well as monocytic differentiation status. Strategies to overcome venetoclax resistance are being developed in clinical trials, including triplet therapies with targeted agents targeting IDH, FLT3, as well as the recently developed menin inhibitors or immunotherapies such as antibody–drug conjugated or monoclonal antibodies. A better understanding of the molecular factors driving venetoclax resistance by single-cell analyses will help the discovery of new therapeutic strategies in the future.
Vascular Morbidity and Mortality in Craniopharyngioma Patients—A Scoping Review
Authors: Beckhaus Julia , Friedrich Carsten , Müller Hermann L.
Published: March 2024

Craniopharyngioma (CP) treatment, including surgery and radiotherapy, can have short- and long-term vascular side effects. Hypothalamic damage is related to morbid obesity and may increase the lifelong risk of experiencing vascular events in CP patients. This review summarized the available evidence regarding vascular complications in adamantinomatous or papillary CP patients, whatever their age at diagnosis. Three databases (Medline, CINAHL, Web of Science) were searched (06/2023) to retrieve eligible articles. The search was limited to peer-reviewed articles. Titles, abstracts, and full texts were screened by two independent reviewers, and data were extracted using a self-developed grid. Seventy-two studies were included in this review; the majority were case reports. Reported vascular sequela that occurred due to surgery were fusiform dilation of the carotid artery, stroke, vasospasm, hemorrhage, and aneurysm. Related conditions that emerged due to radiotherapy included Moyamoya syndrome and cavernoma. Cardiovascular morbidity and mortality often lead to hypothalamic obesity and metabolic syndrome in CP patients. Vascular damage is a rare complication of CP treatment. Surgical strategies should protect the surrounding hypothalamic and vascular structures. Patients receiving radiotherapy, particularly at a young age, should undergo magnetic resonance angiography monitoring to identify possible neurovascular sequela during post-treatment care.
Phosphodiesterases 4B and 4D Differentially Regulate cAMP Signaling in Calcium Handling Microdomains of Mouse Hearts
Authors: Kraft Axel E. , Bork Nadja I. , Subramanian Hariharan , Pavlaki Nikoleta , Failla Antonio V. , Zobiak Bernd , Conti Marco , Nikolaev Viacheslav O.
Published: March 2024

The ubiquitous second messenger 3′,5′-cyclic adenosine monophosphate (cAMP) regulates cardiac excitation-contraction coupling (ECC) by signaling in discrete subcellular microdomains. Phosphodiesterase subfamilies 4B and 4D are critically involved in the regulation of cAMP signaling in mammalian cardiomyocytes. Alterations of PDE4 activity in human hearts has been shown to result in arrhythmias and heart failure. Here, we sought to systematically investigate specific roles of PDE4B and PDE4D in the regulation of cAMP dynamics in three distinct subcellular microdomains, one of them located at the caveolin-rich plasma membrane which harbors the L-type calcium channels (LTCCs), as well as at two sarco/endoplasmic reticulum (SR) microdomains centered around SR Ca2+-ATPase (SERCA2a) and cardiac ryanodine receptor type 2 (RyR2). Transgenic mice expressing Förster Resonance Energy Transfer (FRET)-based cAMP-specific biosensors targeted to caveolin-rich plasma membrane, SERCA2a and RyR2 microdomains were crossed to PDE4B-KO and PDE4D-KO mice. Direct analysis of the specific effects of both PDE4 subfamilies on local cAMP dynamics was performed using FRET imaging. Our data demonstrate that all three microdomains are differentially regulated by these PDE4 subfamilies. Whereas both are involved in cAMP regulation at the caveolin-rich plasma membrane, there are clearly two distinct cAMP microdomains at the SR formed around RyR2 and SERCA2a, which are preferentially controlled by PDE4B and PDE4D, respectively. This correlates with local cAMP-dependent protein kinase (PKA) substrate phosphorylation and arrhythmia susceptibility. Immunoprecipitation assays confirmed that PDE4B is associated with RyR2 along with PDE4D. Stimulated Emission Depletion (STED) microscopy of immunostained cardiomyocytes suggested possible co-localization of PDE4B with both sarcolemmal and RyR2 microdomains. In conclusion, our functional approach could show that both PDE4B and PDE4D can differentially regulate cardiac cAMP microdomains associated with calcium homeostasis. PDE4B controls cAMP dynamics in both caveolin-rich plasma membrane and RyR2 vicinity. Interestingly, PDE4B is the major regulator of the RyR2 microdomain, as opposed to SERCA2a vicinity, which is predominantly under PDE4D control, suggesting a more complex regulatory pattern than previously thought, with multiple PDEs acting at the same location.
Blood Markers Show Neural Consequences of LongCOVID-19
Authors: Tang Norina , Kido Tatsuo , Shi Jian , McCafferty Erin , Ford Judith M. , Dal Bon Kaitlyn , Pulliam Lynn
Published: March 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) persists throughout the world with over 65 million registered cases of survivors with post-COVID-19 sequelae, also known as LongCOVID-19 (LongC). LongC survivors exhibit various symptoms that span multiple organ systems, including the nervous system. To search for neurological markers of LongC, we investigated the soluble biomolecules present in the plasma and the proteins associated with plasma neuronal-enriched extracellular vesicles (nEVs) in 33 LongC patients with neurological impairment (nLongC), 12 COVID-19 survivors without any LongC symptoms (Cov), and 28 pre-COVID-19 healthy controls (HC). COVID-19 positive participants were infected between 2020 and 2022, not hospitalized, and were vaccinated or unvaccinated before infection. IL-1β was significantly increased in both nLongC and Cov and IL-8 was elevated in only nLongC. Both brain-derived neurotrophic factor and cortisol were significantly elevated in nLongC and Cov compared to HC. nEVs from people with nLongC had significantly elevated protein markers of neuronal dysfunction, including amyloid beta 42, pTau181 and TDP-43. This study shows chronic peripheral inflammation with increased stress after COVID-19 infection. Additionally, differentially expressed nEV neurodegenerative proteins were identified in people recovering from COVID-19 regardless of persistent symptoms.
Indoxyl Sulfate-Induced Valve Endothelial Cell Endothelial-to-Mesenchymal Transition and Calcification in an Integrin-Linked Kinase-Dependent Manner
Authors: Delgado-Marin Maria , Sánchez-Esteban Sandra , Cook-Calvete Alberto , Jorquera-Ortega Sara , Zaragoza Carlos , Saura Marta
Published: March 2024

Calcific Aortic Valve Disease (CAVD) is a significant concern for cardiovascular health and is closely associated with chronic kidney disease (CKD). Aortic valve endothelial cells (VECs) play a significant role in the onset and progression of CAVD. Previous research has suggested that uremic toxins, particularly indoxyl sulfate (IS), induce vascular calcification and endothelial dysfunction, but the effect of IS on valve endothelial cells (VECs) and its contribution to CAVD is unclear. Our results show that IS reduced human VEC viability and increased pro-calcific markers RUNX2 and alkaline phosphatase (ALP) expression. Additionally, IS-exposed VECs cultured in pro-osteogenic media showed increased calcification. Mechanistically, IS induced endothelial-to-mesenchymal transition (EndMT), evidenced by the loss of endothelial markers and increased expression of mesenchymal markers. IS triggered VEC inflammation, as revealed by NF-kB activation, and decreased integrin-linked kinase (ILK) expression. ILK overexpression reversed the loss of endothelial phenotype and RUNX2, emphasizing its relevance in the pathogenesis of CAVD in CKD. Conversely, a lower dose of IS intensified some of the effects in EndMT caused by silencing ILK. These findings imply that IS affects valve endothelium directly, contributing to CAVD by inducing EndMT and calcification, with ILK acting as a crucial modulator.
Prevalence of Scoliosis in Children and Adolescents with Cystic Fibrosis
Authors: Fainardi Valentina , Nora Monica , Salghetti Annamaria , Petraglia Federica , Fanzaghi Patrizia , Anelli Giulia , Cattabiani Marta , Scopelliti Giuseppe , Deolmi Michela , Ferrari Ilaria , Longo Francesco , Esposito Susanna , Pisi Giovanna
Published: March 2024

The prevalence of scoliosis in people with cystic fibrosis (CF) seems to be greater than in the normal population. Over the last two years, a screening for spinal deformities was carried out in patients with CF aged 5 to 18 years, followed up at the CF regional Centre in Parma (Italy). Forty-three patients (twenty-seven males, mean age: 11.8 ± 4.5 years) were enrolled in the study. Nine patients (20.9%) were diagnosed with scoliosis, with a mean Cobb angle of 20.8 ± 9.4 (12–38°). Five patients (11.6%) were diagnosed with a postural kyphosis attitude and one with pathological fixed kyphosis. All patients with scoliosis and postural kyphosis started daily physiotherapeutic scoliosis-specific exercises (PSSE). Compared to people without CF, the prevalence of scoliosis in our paediatric CF population seems to be higher and more present in males; the curves were thoracic and mostly right-sided. CF disease, hyposthenic postural attitude and sedentary lifestyle can contribute to the pathogenesis of this musculoskeletal alteration. Spinal deformities may negatively affect pulmonary function, resulting in disability, pain and a decreased quality of life. Since the prevention of musculoskeletal deformities is easier than restoration, in CF population targeted screening during growth and interventions, including regular physical exercise, are mandatory.
Study on Chemical Composition and Biological Activity of Psidium guajava Leaf Extracts
Authors: Park Hyonam , Kim Bohee , Kang Yuri , Kim Woonjung
Published: March 2024

Guava (Psidium guajava) is a plant widely distributed in tropical and subtropical regions. Its leaves contain a large amount of physiological molecules such as flavonoid, sesquiterpene, triterpenoid, coumarin, alkaloid, and tannin molecules with antioxidative and anti-inflammatory effects. In this study, the use of concentrated P. guajava leaf extract molecules as a functional natural material was evaluated by confirming the extract’s antioxidative, antibacterial, tyrosinase activity inhibition, and collagenase activity inhibition effects and its trans-2-nonenal removal ability. As a result of the analysis of the antioxidant and antibacterial components of concentrated P. guajava leaf extract molecules through GC-MS, a large amount of aromatic hydrocarbon molecules were detected. When different concentrations of ethanol were used for extraction, the leaf extract concentrated with 70% ethanol showed the most effective active molecules. As a result of measuring DPPH radical scavenging activity, a concentration-dependent antioxidant activity was confirmed. The antioxidant activity tended to increase when the ethanol content used for extraction was increased. Molecules such as 2,4-di-tert-butylphenol, caryophyllene oxide, and γ-muurolene in P. guajava leaf extract concentrate appeared to have antibacterial activities against S. aureus bacteria known to cause atopy. As ethanol content increased, the inhibitory effect on tyrosinase activity was increased. In addition, when ethanol content was 50%, the concentrated leaf extract was able to remove trans-2-nonenal by 52.4%. As a result of determining the concentrated leaf extract’s collagenase inhibition activity, an inhibition rate close to that of ascorbic acid, a positive control, was confirmed. The concentrated guajava leaf extract molecules were confirmed to have whitening and wrinkle-improving functionality. Thus, the P. guajava leaf extract has high potential as a food and natural cosmetic material.
Increased Apolipoprotein A1 Expression Correlates with Tumor-Associated Neutrophils and T Lymphocytes in Upper Tract Urothelial Carcinoma
Authors: Chang Chih-Chia , Chang Chia-Bin , Chen Chiung-Ju , Tung Chun-Liang , Hung Chi-Feng , Lai Wei-Hong , Shen Cheng-Huang , Tsai Chang-Yu , Lai Ya-Yan , Lee Ming-Yang , Wu Shu-Fen , Chen Pi-Che
Published: March 2024

An increased neutrophil-to-lymphocyte ratio (NLR) is a poor prognostic biomarker in various types of cancer, because it reflects the inhibition of lymphocytes in the circulation and tumors. In urologic cancers, upper tract urothelial carcinoma (UTUC) is known for its aggressive features and lack of T cell infiltration; however, the association between neutrophils and suppressed T lymphocytes in UTUC is largely unknown. In this study, we examined the relationship between UTUC-derived factors and tumor-associated neutrophils or T lymphocytes. The culture supernatant from UTUC tumor tissue modulated neutrophils to inhibit T cell proliferation. Among the dominant factors secreted by UTUC tumor tissue, apolipoprotein A1 (Apo-A1) exhibited a positive correlation with NLR. Moreover, tumor-infiltrating neutrophils were inversely correlated with tumor-infiltrating T cells. Elevated Apo-A1 levels in UTUC were also inversely associated with the population of tumor-infiltrating T cells. Our findings indicate that elevated Apo-A1 expression in UTUC correlates with tumor-associated neutrophils and T cells. This suggests a potential immunomodulatory effect on neutrophils and T cells within the tumor microenvironment, which may represent therapeutic targets for UTUC treatment.
Inflammation Factors and Genistein Supplementation in Cancer—Preliminary Research
Authors: Banyś Karolina , Jelińska Małgorzata , Wrzosek Małgorzata , Skrajnowska Dorota , Wrzesień Robert , Bielecki Wojciech , Bobrowska-Korczak Barbara
Published: March 2024

The purpose of this study was to evaluate the effect of genistein in nano, micro, and macro forms on the intensity of the DMBA-induced tumor process in rats and to understand the mechanisms of this action. The effect of genistein supplementation on the content of selected eicosanoids (HETEs, HODE, and HEPE) in the serum of rats was evaluated. The levels and expression of genes encoding various pro-inflammatory cytokines (IL-1, IL-6) and MMP-9 in the blood of rats were also investigated. The biological material for the study was blood obtained from female rats of the Sprague Dawley strain (n = 32). The animals were randomly divided into four groups: animals without supplementation, and animals supplemented at a dose of 0.2 mg/kg b.w. (0.1 mg/mL) with macro, micro (587 ± 83 nm), or nano (92 ± 41 nm) genistein. To induce mammary neoplasia (adenocarcinoma), rats were given 7,12-dimethyl-1,2-benz[a]anthracene (DMBA). The content of selected eicosanoids was determined by liquid chromatography with UV detection. An immunoenzymatic method was used to determine the content of cytokines and MMP-9. The expression of the IL-6, IL-1beta, and MMP-9 genes was determined with quantitative real-time PCR (qRT-PCR) using TaqMan probes. Based on the study, it was shown that supplementation of animals with genistein in macro, micro, and nano forms increased the intensity of the tumor process in rats. It was shown that the content of 12-HEPE, HODE, and 12-HETE in the serum of genistein-supplemented rats was statistically significantly lower with respect to the content of the aforementioned markers in the serum of rats receiving only a standard diet, devoid of supplementation. It was found that animals supplemented with nano-, micro-, and macrogenistein had higher levels of metalloproteinase-9, MMP-9, compared to animals without supplementation. There was a significant increase in MMP-9 gene expression in the blood of macrogenistein-supplemented animals, relative to the other groups of rats. On the basis of the study, it was shown that supplementation of animals with nano-, micro-, and macrogenistein had an effect on the development of the tumor process. Dietary supplementation with genistein significantly decreased the level of selected eicosanoids, which may have significant impacts on cancer development and progression.
Multifaceted Linkages among Eco-Climatic Factors, Plankton Abundance, and Gonadal Maturation of Hilsa Shad, Tenualosa ilisha, Populations in Bangladesh
Authors: Shohan Mobin Hossain , Siddique Mohammad Abu Baker , Mahalder Balaram , Haque Mohammad Mahfujul , Goswami Chayon , Ahmed Md. Borhan Uddin , Alam Mohammad Ashraful , Bashar Md. Abul , Mahmud Yahia , Chowdhury Mahamudul Alam , Hasan Md. Mahmudul , Ahammad A. K. Shakur
Published: March 2024

An integrated multivariate approach was applied to gain a deeper understanding of the feeding biology of hilsa shad, Tenualosa ilisha, collected from six different aquatic habitats across Bangladesh. This approach involved linking climatic factors, ecological factors, plankton abundance in water, reproductive traits, and plankton ingestion data. Climatic data were obtained from the National Oceanic and Atmospheric Administration (NOAA) and Climate Data Online (CDO) databases on a monthly basis. Water quality parameters were observed on-site at various sampling sites. Plankton data from water bodies and hilsa guts were collected monthly from the study areas and analyzed in the laboratory. The results obtained were averaged for each month. The correlation tests, multivariate approaches, cluster analyses, and regression analyses revealed that the gonadosomatic index was primarily influenced by climatic factors, the abundance of ingested gut plankton, and heir compositions. The analysis of selectivity indices confirmed that plankton preferentially ingested selective taxa. Thirteen plankton groups were identified in the water column of six different hilsa habitats. The dominant phytoplankton groups were Bacillariophyceae (34–53%), Chlorophyceae (31–50%), Cyanophyceae (4–8%), and Euglenophyceae (1–3%). Additionally, Copepoda, Rotifera, and Cladocera were the most numerous zooplankton groups. Hilsa shad primarily consumed Bacillariophyceae (38–57%), Chlorophyceae (35–53%), and Cyanophyceae (4–6%). However, they also exhibited selective ingestion of higher quantities of Bacillariophyceae and Chlorophyceae to fulfill specific and unique metabolic needs. Cluster analysis revealed the variability of phytoplankton and zooplankton abundance in water and gut in relation to diverse locations. Combining all the datasets, a principal component analysis (PCA) was applied. The first two principal components explained more than 54% of the variability. The first component explained the association between the gonadosomatic index and eco-climatic factors, while the second component extracted the cluster of ingested gut plankton in relation to salinity and pH. Pearson’s correlations and linear regression analyses showed that the number of gut plankton had a positive influence on the gonadosomatic index (GSI). Finally, the outcomes from these extensive datasets have provided a better understanding of the selective feeding behavior and the influence of feeding biology on the gonadal maturation of T. ilisha. This understanding is likely to be useful for maintaining and improving the growth and productivity of the existing production systems for this transboundary species.
Graphene Oxide as a Factor Modifying the Properties of Wood
Authors: Betlej Izabela , Andres Bogusław , Borysiak Sławomir , Jaworski Sławomir , Kutwin Marta , Krajewski Krzysztof , Boruszewski Piotr
Published: March 2024

This work carried out research to determine the possibilities of using graphene oxide to provide wood with new functional features. With the saturation parameters used and working liquid with a concentration of 0.004% graphene oxide, the retention of the nanomaterial in wood was 0.25 kg/m3. The presence of graphene oxide increased the crystallinity of the wood to 64% (compared with 57% for unmodified wood). The TG/DTG spectra of wood impregnated with graphene oxide and the control wood indicated that the initial weight loss of the samples observed at a temperature of 100 °C was similar and amounted to less than 4%. A second mass loss was observed in a temperature range of 270 to 380 °C. The mass loss in this temperature range reached 70% and was similar in the test and control samples. Wood modified with graphene oxide showed increased thermal stability in a temperature range of 360 to 660 °C compared with native wood. Given the results obtained, there were no statistically significant differences in the water absorption of modified or control wood. The presence of low concentrations of graphene oxide in the culture medium did not inhibit the growth of the fungus Trichoderma viride; however, a decrease in the growth activity of mycelial hyphae was observed with an increasing concentration of nanomaterial in the medium. It has been reported that graphene oxide, as a stress factor, initiates changes at the cellular level, characterized by the formation of structures called chlamydospores by the body.
Emerging Trends in Smart Self-Healing Coatings: A Focus on Micro/Nanocontainer Technologies for Enhanced Corrosion Protection
Authors: Sanyal Simpy , Park SeonJu , Chelliah Ramachandran , Yeon Su-Jung , Barathikannan Kaliyan , Vijayalakshmi Selvakumar , Jeong Ye-Jin , Rubab Momna , Oh Deog Hawn
Published: March 2024

Smart self-healing coatings offer a revolutionary approach to mitigating metal corrosion, a problem with significant economic and environmental impacts. Divided into intrinsic and extrinsic types, these coatings autonomously rectify the damage. Intrinsic variants utilize reversible bonds to achieve ongoing repair, while extrinsic ones incorporate micro/nanocontainers that activate upon environmental triggers to mend micro-cracks, their efficacy dictated by the encapsulated healing agents’ volume. This review dissects the rapidly evolving sector of stimuli-responsive self-healing coatings, emphasizing the progress in micro/nano container technology. It discusses the synthesis and encapsulation processes of different micro/nanocontainers and charts the transition from single to multistimulus-responsive systems, which enhances the coatings’ sensitivity and functionality. The addition of multifunctional traits such as self-reporting and anti-microbial actions further broadens their industrial applicability. The review provides a succinct overview of the field’s current state and future potential, envisioning a paradigm shift in corrosion protection through advanced smart coatings.
Olive Leaves and Citrus Peels: From Waste to Potential Resource for Cosmetic Products
Authors: d’Avanzo Nicola , Mancuso Antonia , Mare Rosario , Silletta Antonio , Maurotti Samantha , Parisi Ortensia Ilaria , Cristiano Maria Chiara , Paolino Donatella
Published: March 2024

Resource depletion and food waste accumulation represent a tremendous socio-economic and environmental problem. One promising strategy involves the use of byproducts derived from food waste as ingredients for cosmetic products. The aim of this work is to propose clementine peels and olive leaf extracts as value-added bioproducts for a cosmetic cream. Extracts were obtained by super critical extraction showing an antioxidant activity of ca. 25%. No cytotoxic effects of the extracts were recorded on keratinocyte cells up to a concentration of 4% v/v ratio within 24 h. The incorporation of clementine peels and olive leaf extracts into creams did not compromise their stability, as demonstrated by Turbiscan analyses at room and extreme (40 °C) storage conditions. The safety profiles of the final cosmetic formulations were further in vivo demonstrated on human volunteers. We analyzed the trans-epidermal water loss and variation of the skin’s erythematous index, which showed profiles that almost overlapped with the negative control. Moreover, rheological analysis of the resulting creams evidences their suitable spreadability with similar pseudoplastic profiles, although a slight reduction of viscosity was recorded by improving the extracts’ concentrations. The proposed approach highlights the advantage of combining byproduct resources and supercritical fluid extraction to obtain a safe and eco-friendly face cream.
Automatic Detection of Cast Billet Dendrite Based on Improved Hough Transform
Authors: Wang Yuhan , He Qing , Xie Zhi
Published: March 2024

Primary dendrite information is one of the most important metrics to measure the quality of continuous cast slabs. The contrast of low magnification images is very low under the influence of illumination and sampling devices, so the traditional dendrite detection method has the problem of missed detections. We propose an automatic dendrite detection method based on an improved Hough transform, which effectively improves the accuracy and efficiency of primary dendrite detection. By using the local grayscale features of the image, a genetic algorithm-based local contrast enhancement algorithm is proposed. Compared with the traditional contrast enhancement algorithm, it can retain all the information of the dendrites. Combined with the image binarization method based on Hessian matrix, we can obtain more detailed information about the dendrites. According to the continuity and solidification characteristics of dendrites, the Hough transform is improved to extract dendrite information, which effectively reduces the computational cost of the Hough transform. The experimental results show that the method of this paper has versatility, and the error is four pixels compared with the manual method, which can provide a reliable basis for the subsequent judgement of the quality of cast billets.
Cell Therapy Transplant Canada (CTTC) Consensus-Based Guideline 2024 for Management and Treatment of Chronic Graft-Versus-Host Disease and Future Directions for Development
Authors: Kim Dennis Dong Hwan , Popradi Gizelle , Lepic Kylie , Paulson Kristjan , Allan David , Nampoothiri Ram Vasudevan , Lachance Sylvie , Deotare Uday , White Jennifer , Elemary Mohamed , Jamani Kareem , Fraga Christina , Lemieux Christopher , Novitzky-Basso Igor , Law Arjun Datt , Kumar Rajat , Walker Irwin , Schultz Kirk R.
Published: March 2024

This is a consensus-based Canadian guideline whose primary purpose is to standardize and facilitate the management of chronic graft-versus-host disease (cGvHD) across the country. Creating uniform healthcare guidance in Canada is a challenge for a number of reasons including the differences in healthcare authority structure, funding and access to healthcare resources between provinces and territories, as well as the geographic size. These differences can lead to variable and unequal access to effective therapies for GvHD. This document will provide comprehensive and practical guidance that can be applied across Canada by healthcare professionals caring for patients with cGvHD. Hopefully, this guideline, based on input from GvHD treaters across the country, will aid in standardizing cGvHD care and facilitate access to much-needed novel therapies. This consensus paper aims to discuss the optimal approach to the initial assessment of cGvHD, review the severity scoring and global grading system, discuss systemic and topical treatments, as well as supportive therapies, and propose a therapeutic algorithm for frontline and subsequent lines of cGvHD treatment in adults and pediatric patients. Finally, we will make suggestions about the future direction of cGvHD treatment development such as (1) a mode-of-action-based cGvHD drug selection, according to the pathogenesis of cGvHD, (2) a combination strategy with the introduction of newer targeted drugs, (3) a steroid-free regimen, particularly for front line therapy for cGvHD treatment, and (4) a pre-emptive approach which can prevent the progression of cGvHD in high-risk patients destined to develop severe and highly morbid forms of cGvHD.
Effect of Calving Season on Productive Performance of Dairy Cows
Authors: Stojnov Martin , Penev Toncho , Dimov Dimo , Marinov Ivaylo
Published: March 2024

The aim of the present research was to study the influence of the calving season in conditions of the upcoming climate changes on the productive traits of dairy cows in Bulgaria. The study was conducted on a cattle farm with a capacity of 500 dairy cows, which were loose-housed in open free-stall barns (shed-type). In the research, 286 lactations of 199 Holstein cattle from the studied farm were included. The cows with the highest average milk yield for lactation—8522.2 kg—calved in the spring, while the cows with the lowest milk yield—8082.7 kg—calved in the summer. Cows that calved in the spring had the highest maximum daily milk yield (lactation peak)—38 kg—whereas cows that calved in the summer had the lowest—35.7 kg. Regarding the composition indicators of milk, fat, and protein content, no significant effect of the calving season was found, but there was a tendency for the lowest values for the percentage of fat in milk to be reported for cows that calved in the summer—3.68%—and the highest for those calved in the spring—3.71%. Regarding the percentage of protein in the milk, the lowest values were observed for cows that calved in autumn—3.19%—and the highest for cows that calved in summer—3.27%.
Automatic Wake and Deep-Sleep Stage Classification Based on Wigner–Ville Distribution Using a Single Electroencephalogram Signal
Authors: Yeh Po-Liang , Ozgoren Murat , Chen Hsiao-Ling , Chiang Yun-Hong , Lee Jie-Ling , Chiang Yi-Chen , Chiang Rayleigh Ping-Ying
Published: March 2024

This research paper outlines a method for automatically classifying wakefulness and deep sleep stage (N3) based on the American Academy of Sleep Medicine (AASM) standards. The study employed a single-channel EEG signal, leveraging the Wigner–Ville Distribution (WVD) for time–frequency analysis to determine EEG energy per second in specific frequency bands (δ, θ, α, and entire band). Particle Swarm Optimization (PSO) was used to optimize thresholds for distinguishing between wakefulness and stage N3. This process aims to mimic a sleep technician’s visual scoring but in an automated fashion, with features and thresholds extracted to classify epochs into correct sleep stages. The study’s methodology was validated using overnight PSG recordings from 20 subjects, which were evaluated by a technician. The PSG setup followed the 10–20 standard system with varying sampling rates from different hospitals. Two baselines, T1 for the wake stage and T2 for the N3 stage, were calculated using PSO to ascertain the best thresholds, which were then used to classify EEG epochs. The results showed high sensitivity, accuracy, and kappa coefficient, indicating the effectiveness of the classification algorithm. They suggest that the proposed method can reliably determine sleep stages, being aligned closely with the AASM standards and offering an intuitive approach. The paper highlights the strengths of the proposed method over traditional classifiers and expresses the intentions to extend the algorithm to classify all sleep stages in the future.
Association between Dietary Practice and Gait Speed in Community-Dwelling Older Adults with Overweight and Obesity: A Cross-Sectional Study
Authors: Itani Leila , Pellegrini Massimo , Saadeddine Dana , Samouda Hanen , Kreidieh Dima , Tannir Hana , El Ghoch Marwan
Published: March 2024

Slow gait speed is associated with poorer clinical outcomes and higher rates of functional limitation and mortality in older adults, especially when combined with overweight or obesity. Aging is also associated with nutritional deficits. The aim of our study was to assess the potential association between dietary practice and gait speed performance in community-dwelling older adults with overweight and obesity. Participants underwent body composition measurement with the Tanita MC-780MA Bioimpedance Analyzer (BIA). Dietary patterns were assessed with the Mini Nutritional Assessment (MNA) questionnaire, and a dietary adequacy (DA) score system was constructed. The four-meter gait speed test was performed in order to assess gait speed. Of 222 participants, aged 67.6 ± 6.6 years, with a body mass index (BMI) of 31.9 ± 4.5 kg/m2, 34.7% had reduced gait speed and lower DA compared to those with normal gait speed (2.99 ± 1.12 vs. 3.37 ± 1.07; p < 0.05). The DA score of participants with slower gait speed was more likely to fall below the median than that of participants with normal gait speed (70.1% vs. 51.7%; p < 0.05). Participants with slower gait speed were more likely to be nutritionally at risk of low DA (22.1% vs. 10.3%; p < 0.05). Logistic regression analysis, after adjustment for confounders, showed that the risk of having a slow gait speed was 75% lower among those with a higher DA score (OR = 0.25; 95% CI = 0.11–0.53). Older adults with overweight or obesity in community dwellings might need to be supported with nutritional interventions that can improve their gait speed.
Hybrid Mode: Routinization of the Transition Mode as the Third Common Mode for Compound VTOL Drones
Authors: Hu Jiahao , Wei Jingbo , Liu Kun , Yu Xiaobin , Cao Mingzhi , Qin Zijie
Published: March 2024

Fixed-wing Vertical Takeoff and Landing (VTOL) drones have been widely researched and applied because they combine the advantages of both rotorcraft and fixed-wing drones. However, the research on the transition mode of this type of drone has mainly focused on completing the process quickly and stably, and the application potential of this mode has not been given much attention. The objective of this paper is to routinize the transition mode of compound VTOL drones, i.e., this mode works continuously for a longer period of time as a third commonly used mode besides multi-rotor and fixed-wing modes, which is referred to as the hybrid mode. For this purpose, we perform detailed dynamics modeling of the drone in this mode and use saturated PID controllers to control the altitude, velocity, and attitude of the drone. In addition, for more stable altitude control in hybrid mode, we identify the relevant parameters for the lift of the fixed-wings and the thrust of the actuators. Simulation and experimental results show that the designed control method can effectively control the compound VTOL drone in hybrid mode. Moreover, it is proven that flight in hybrid mode can reduce the flight energy consumption to some extent.