bluuebunny/crossref_metadata_2025 huggingface dataset:
Do We Need Radiotherapy in Grade II Ependymoma?
Authors: Napieralska Aleksandra , Majewski Wojciech , Miszczyk Leszek
Published: March 2022

Purpose/Objectives

The debate on whether radiotherapy (RT) is an essential part of primary treatment in patients with grade II ependymoma (G2E) is still ongoing, and this study aimed to evaluate its role.

Materials/Methods

A retrospective analysis of all the consecutive patients treated due to G2E in years 1985–2019 was performed. The group consisted of 116 patients with a small predominance of woman (55% vs. 45%) and the location of the tumor in the brain (58% vs. 42%). All had surgery as the primary treatment with 47% R0 resection. Radical RT was applied in 81 patients. In majority of cases (91%), patients received local irradiation.

Results

Median follow-up was 65 months, and during that time, 17 patients died. Five- and 10-year overall survival (OS) of the whole group was 87% and 83%. Radical surgery (R0 vs. R1/2) improved OS (p = 0.004), but the difference was observed only in patients with brain lesions (p = 0.01). Five- and 10-year progression-free survival (PFS) was 68% and 51%, respectively. Looking at the treatment of recurrence, those who received RT as a part of the treatment of the recurrent tumor had better OS (p = 0.048)—5- and 10-year OS of 85% and 78% vs. 66% and 57%. In the multivariate analysis, radical surgery (R0 vs. R1/2) and the use of RT in the primary treatment improved PFS (p = 0.006 and 0.007). Based on the location of the tumor, the positive influence of RT on PFS was observed only in the case of patients with brain tumors (p = 0.01). Also, comparing R1/2 surgery with R0 resection—the benefit of RT was only observed in R1/2 group (0.02).

Conclusions

RT in the case of patients with G2E is a valuable treatment of the recurrent disease. Patients with brain lesions after nonradical surgery might benefit from the local irradiation in terms of PFS.
Clinical Application of Indocyanine Green Fluorescence Technology in Laparoscopic Radical Gastrectomy
Authors: Wei Meng , Liang Yize , Wang Limei , Li Zhen , Chen Yuanyuan , Yan Zhibo , Sun Danping , Huang Yadi , Zhong Xin , Liu Peng , Yu Wenbin
Published: March 2022

Background

This study aimed to observe the application and evaluate the feasibility and safety of indocyanine green (ICG) fluorescence technology in laparoscopic radical gastrectomy (LRG).

Methods

Patients who underwent LRG & D2 lymphadenectomy at Qilu Hospital of Shandong University were included between January 2018 and August 2019. According to whether endoscopic injection of ICG was performed, patients were assigned to the ICG group (n=107) and the control group (n=88). The clinicopathologic features, retrieved lymph nodes, postoperative recovery, and follow-up data were compared between the two groups.

Results

Baseline characteristics are comparable. The ICG group had a significantly larger number of lymph nodes retrieved (49.55 ± 12.72 vs. 44.44 ± 10.20, P<0.05), shorter total operation time (min) (198.22 ± 13.14 vs. 202.50 ± 9.91, P<0.05), shorter dissection time (min) (90.90 ± 5.34 vs. 93.74 ± 5.35, P<0.05) and less blood loss (ml) (27.51 ± 12.83 vs. 32.02 ± 17.99, P<0.05). The median follow-up time was 29.0 months (range 1.5-43.8 months), and there was no significant difference between the ICG group and the control group in 2-year OS (87.8% vs. 82.9%, P>0.05) or DFS (86.0% vs. 80.7%, P>0.05).

Conclusions

ICG fluorescence technology in laparoscopic radical gastrectomy has advantages in LN dissection, operation time, and intraoperative blood loss. The 2-year OS and 2-year DFS rates between the two groups were comparable. In conclusion, ICG fluorescence technology is feasible and safe.
An Ultrasonic-Based Radiomics Nomogram for Distinguishing Between Benign and Malignant Solid Renal Masses
Authors: Li Chunxiang , Qiao Ge , Li Jinghan , Qi Lisha , Wei Xueqing , Zhang Tan , Li Xing , Deng Shu , Wei Xi , Ma Wenjuan
Published: March 2022

Objectives

This study was conducted in order to develop and validate an ultrasonic-based radiomics nomogram for diagnosing solid renal masses.

Methods

Six hundred renal solid masses with benign renal lesions (n = 204) and malignant renal tumors (n = 396) were divided into a training set (n = 480) and a validation set (n = 120). Radiomics features were extracted from ultrasound (US) images preoperatively and then a radiomics score (RadScore) was calculated. By integrating the RadScore and independent clinical factors, a radiomics nomogram was constructed. The diagnostic performance of junior physician, senior physician, RadScore, and radiomics nomogram in identifying benign from malignant solid renal masses was evaluated based on the area under the receiver operating characteristic curve (ROC) in both the training and validation sets. The clinical usefulness of the nomogram was assessed using decision curve analysis (DCA).

Results

The radiomics signature model showed satisfactory discrimination in the training set [area under the ROC (AUC), 0.887; 95% confidence interval (CI), 0.860–0.915] and the validation set (AUC, 0.874; 95% CI, 0.816–0.932). The radiomics nomogram also demonstrated good calibration and discrimination in the training set (AUC, 0.911; 95% CI, 0.886–0.936) and the validation set (AUC, 0.861; 95% CI, 0.802–0.921). In addition, the radiomics nomogram model showed higher accuracy in discriminating benign and malignant renal masses compared with the evaluations by junior physician (DeLong p = 0.004), and the model also showed significantly higher specificity than the senior and junior physicians (0.93 vs. 0.57 vs. 0.46).

Conclusions

The ultrasonic-based radiomics nomogram shows favorable predictive efficacy in differentiating solid renal masses.
Laparoscopic Gastrectomy for Cancer: Cut Down Complications to Unveil Positive Results of Minimally Invasive Approach
Authors: Bjelovic Milos , Veselinovic Milan , Gunjic Dragan , Bukumiric Zoran , Babic Tamara , Vlajic Radmila , Potkonjak Dario
Published: March 2022

Several randomized controlled trials and meta-analyses have confirmed the advantages of laparoscopic surgery in early gastric cancer, and there are indications that this may also apply in advanced distal gastric cancer. The study objective was to evaluate the safety and effectiveness of laparoscopic gastrectomy (LG), in comparison to open gastrectomy (OG), in the management of locally advanced gastric cancer. The single-center, case–control study included 204 patients, in conveyance sampling, who underwent radical gastrectomy for locally advanced gastric cancer. Out of 204 patients, 102 underwent LG, and 102 patients underwent OG. The primary endpoints were safety endpoints, i.e., complication rates, reoperation rates, and 30-day mortality rates. The secondary endpoints were efficacy endpoints, including perioperative characteristics and oncological outcomes. Even though the overall complication rate was higher in the OG group compared to the LG group (30.4% and 19.6%, respectively), the difference between groups did not reach statistical significance (p = 0.075). No significant difference was identified in reoperation rates and 30-day mortality rates. Time spent in the intensive care unit (ICU) and overall hospital stay were shorter in the LG group compared to the OG group (p < 0.001). Although the number of retrieved lymph nodes is oncologically adequate in both groups, the median number is higher in the OG group (35 vs. 29; p = 0.024). Resection margins came out to be negative in 92% of patients in the LG group and 73.1% in the OG group (p < 0.001). The study demonstrated statistically longer survival rates for the patients in the laparoscopic group, which particularly applies to patients in the most prevalent, third stage of the disease. When patients with the Clavien–Dindo grade ≥II were excluded from the survival analysis, further divergence of survival curves was observed. In conclusion, LG can be safely performed in patients with locally advanced gastric cancer and accomplish the oncological standard with short ICU and overall hospital stay. Since postoperative complications could affect overall treatment results and diminish and blur the positive effect of the minimally invasive approach, further clinical investigations should be focused on the patients with no surgical complications and on clinical practice to cut down the prevalence of complications.
Common Molecular Pathways Between Post-COVID19 Syndrome and Lung Fibrosis: A Scoping Review
Authors: Bergantini Laura , Mainardi Alessandro , d’Alessandro Miriana , Cameli Paolo , Bennett David , Bargagli Elena , Sestini Piersante
Published: March 2022

The pathogenetic mechanism of post-Covid-19 pulmonary fibrosis is currently a topic of intense research interest, but still largely unexplored. The aim of this work was to carry out a systematic exploratory search of the literature (Scoping review) to identify and systematize the main pathogenetic mechanisms that are believed to be involved in this phenomenon, in order to highlight the same molecular aspect of the lung. These aims could be essential in the future for therapeutic management. We identified all primary studies involving in post COVID19 syndrome with pulmonary fibrosis as a primary endpoint by performing data searches in various systematic review databases. Two reviewers independently reviewed all abstracts (398) and full text data. The quality of study has been assess through SANRA protocol. A total of 32 studies involving were included, included the possible involvement of inflammatory cytokines, concerned the renin-angiotensin system, the potential role of galectin-3, epithelial injuries in fibrosis, alveolar type 2 involvement, Neutrophil extracellular traps (NETs) and the others implied other specific aspects (relationship with clinical and mechanical factors, epithelial transition mesenchymal, TGF-β signaling pathway, midkine, caspase and macrophages, genetics). In most cases, these were narrative reviews or letters to the editor, except for 10 articles, which presented original data, albeit sometimes in experimental models. From the development of these researches, progress in the knowledge of the phenomenon and hopefully in its prevention and therapy may originate.
Beneficial Metabolic Effects of Praliciguat, a Soluble Guanylate Cyclase Stimulator, in a Mouse Diet-Induced Obesity Model
Authors: Schwartzkopf Chad D. , Hadcock John R. , Liu Guang , Germano Peter , Roux Julien , Shea Courtney M. , Buys Emmanuel S. , Jones Juli E.
Published: March 2022

Praliciguat is a soluble guanylate cyclase stimulator that elicits hemodynamic, anti-inflammatory, and antifibrotic effects in preclinical models of metabolic dysfunction. We assessed the metabolic effects of praliciguat in a mouse diet-induced obesity (DIO) model housed at thermoneutrality. At 6 weeks old, male C57BL/6N mice were either maintained on low-fat diet (LFD, lean mice) or placed on 60% high-fat diet (HFD, DIO mice). At 14 weeks old, the DIO mice were either maintained on HFD or switched to HFD with praliciguat (6-mg/kg). Day 28 samples were collected for biomarker analysis. In a second study under the same paradigm, indirect calorimetry was performed on days 8, 9, 20, 21, 32, and 33 and an oral lipid tolerance test (LTT) on day 38. Mice treated 28 days with praliciguat had lower levels of fasting plasma insulin, C-peptide, triglycerides, and HOMA-IR (homeostatic model assessment for insulin resistance) than DIO controls. In addition, energy expenditure was higher in praliciguat-treated than in DIO control mice on days 9, 20, 32, and 33; and day-38 triglycerides were lower. HFD-induced increases in gene expression of liver TNF-ɑ, lipoprotein lipase (Lpl), and patatin-like phospholipase domain-containing protein 3 (Pnpla3) in control DIO mice were attenuated in praliciguat-treated DIO mice. The positive metabolic effects observed in praliciguat-treated mice were associated with the restoration of liver PI3K (pAKT-Thr308) signaling, but not MAPK (pERK). In conclusion, praliciguat-treated DIO mice had increased energy utilization, improved insulin sensitivity, and lower plasma triglycerides. These results illustrate metabolic effects associated with praliciguat treatment in DIO mice.
Acute Histological Chorioamnionitis and Birth Weight in Pregnancies With Preterm Prelabor Rupture of Membranes: A Retrospective Cohort Study
Authors: Matulova Jana , Kacerovsky Marian , Hornychova Helena , Stranik Jaroslav , Mls Jan , Spacek Richard , Burckova Hana , Jacobsson Bo , Musilova Ivana
Published: March 2022

Aim: To assess the association between the birth weight of newborns from pregnancies with preterm prelabor rupture of membranes (PPROM) and the presence of acute histological chorioamnionitis (HCA) with respect to the: i) fetal and maternal inflammatory responses and ii) acute inflammation of the amnion.

Material and Methods: This retrospective cohort study included 818 women with PPROM. A histopathological examination of the placenta was performed. Fetal inflammatory response was defined as the presence of any neutrophils in umbilical cord (histological grades 1–4) and/or chorionic vasculitis (histological grade 4 for the chorionic plate). Maternal inflammatory response was defined as the presence of histological grade 3–4 for the chorion-decidua and/or grade 3 for the chorionic plate and/or grade 1–4 for the amnion. Acute inflammation of the amnion was defined as the presence of any neutrophils in the amnion (histological grade 1–4 for the amnion). Birth weights of newborns were expressed as percentiles derived from INTERGROWTH-21st standards for the i) estimated fetal weight and ii) newborn birth weight.

Results: No difference in percentiles of birth weights of newborns was found among the women with the women with HCA with fetal inflammatory response, with HCA with maternal inflammatory response and those without HCA. Women with HCA with acute inflammation of the amnion had lower percentiles of birth weights of newborns, derived from the estimated fetal weight standards, than women with HCA without acute inflammation of the amnion and those with the absence of HCA in the crude (with acute inflammation: median 46, without acute inflammation: median 52, the absence of HCA: median 55; p = 0.004) and adjusted (p = 0.02) analyses. The same subset of pregnancies exhibited the highest rate of newborns with a birth weight of ≤25 percentile. When percentiles were derived from the newborn weight standards, no differences in birth weights were observed among the subgroups.

Conclusion: Acute inflammation of the amnion was associated with a lower birth weight in PPROM pregnancies, expressed as percentiles derived from the estimated fetal weight standards.
Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis
Authors: Jia Miao , Qiu Hong , Lin Lihua , Zhang Shun , Li Damei , Jin Donghua
Published: March 2022

Peritoneal dialysis (PD) is an important part of replacement therapy for kidney failure. However, long-term PD treatment can cause peritoneal fibrosis. Autophagy may be involved in the pathological mechanism of peritoneal fibrosis (PF). Although autophagy is currently known to be involved in course of PF, its specific effects still lack in-depth research. In this experiment, a high-glucose (HG)-induced peritoneal fibrosis rat model was successfully established via intraperitoneal injection of HG peritoneal dialysate, and the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 and the mechanistic target of rapamycin (mTOR) inhibitor rapamycin were used to treat peritoneal fibrosis rats. In addition, in vitro studies of high glucose-induced peritoneal fibrosis were performed using rat peritoneal mesothelial cells (PMCs). In vivo and in vitro experiments showed that LY294002 and rapamycin effectively inhibited the process of PF induced by high glucose. In addition, LY294002 and rapamycin were found to alleviate fibrosis by eliminating intracellular reactive oxygen species (ROS) levels, promoting the expression of the epithelial mesenchymal transdifferentiation proteins zonula occludens-1 (ZO-1) and E-cadherin, and inhibiting the expression of p-PI3K, PI3K, p-mTOR, mTOR, the fibroblast-specific proteins ferroptosis suppressor protein 1 (FSP1), and alpha-smooth muscle actin (α-SMA). Moreover, LY294002 and rapamycin promoted expression of autophagy-related proteins LC3-II/I, p62, and beclin-1. The current data indicated that inhibition of PI3K/AKT/mTOR signalling pathway activated autophagy and suppressed PF in the process of PD. Therefore, intervention in this signalling pathway may become a research goal for the prevention and treatment of PF, which has important clinical significance.
Effects of an App-Based Physical Exercise Program on Selected Parameters of Physical Fitness of Females in Retirement: A Randomized Controlled Trial
Authors: Jungreitmayr Sonja , Kranzinger Christina , Venek Verena , Ring-Dimitriou Susanne
Published: March 2022

Modern technologies enable new options in the delivery of physical exercise programs. Specially designed app-based programs can be used to help older people in particular to integrate physical exercise into their daily lives. This study examines the influence of an app-based physical exercise program on selected parameters of physical fitness, such as muscular strength, balance, and flexibility. The women (n = 110) were on average 65.3 (± 1.5) years old and, compared to age-specific norm values, healthy. The 14-week intervention consisted of an app-based, unsupervised physical exercise program, in which the exercise frequency and duration of sessions were self-selected. The physical exercise program consisted of simple, functional exercises such as arm circles, squats, lateral raises. The participants were provided with an elastic resistance band and an exercise ball allowing them to increase exercise intensity if needed. Participants were randomly assigned to intervention group (IG) and control group (CG). 71% of the IG used the physical exercise program at least 1.2 times per week, whereas 25% of the IG showed usage rates above four times per week. Significant effects were found in the domains of muscular strength and flexibility. While IG could maintain their performance in isometric muscular strength tests and increased their flexibility, CG faced a decrease in those parameters. Thus, this app-based physical exercise program had positively influenced muscular strength and flexibility in women over 60 years of age.
Nitrogen Deposition Shifts Grassland Communities Through Directly Increasing Dominance of Graminoids: A 3-Year Case Study From the Qinghai-Tibetan Plateau
Authors: Shen Hao , Dong Shikui , DiTommaso Antonio , Xiao Jiannan , Lu Wen , Zhi Yangliu
Published: March 2022

Nitrogen (N) deposition has been increasing for decades and has profoundly influenced the structure and function of grassland ecosystems in many regions of the world. However, the impact of N deposition on alpine grasslands is less well documented. We conducted a 3-year field experiment to determine the effects of N deposition on plant species richness, composition, and community productivity in an alpine meadow of the Qinghai-Tibetan Plateau of China. We found that 3 years of N deposition had a profound effect on these plant community parameters. Increasing N rates increased the dominance of graminoids and reduced the presence of non-graminoids. Species richness was inversely associated with aboveground biomass. The shift in plant species and functional group composition was largely responsible for the increase in productivity associated with N deposition. Climatic factors also interacted with N addition to influence productivity. Our findings suggest that short-term N deposition could increase the productivity of alpine meadows through shifts in composition toward a graminoid-dominated community. Longer-term studies are needed to determine if shifts in composition and increased productivity will be maintained. Future work must also evaluate whether decreasing plant diversity will impair the long-term stability and function of sensitive alpine grasslands.
Function Analysis of the ERF and DREB Subfamilies in Tomato Fruit Development and Ripening
Authors: Zhang Li , Chen LiJing , Pang ShengQun , Zheng Qun , Quan ShaoWen , Liu YuFeng , Xu Tao , Liu YuDong , Qi MingFang
Published: March 2022

APETALA2/ethylene responsive factors (AP2/ERF) are unique regulators in the plant kingdom and are involved in the whole life activity processes such as development, ripening, and biotic and abiotic stresses. In tomato (Solanum lycopersicum), there are 140 AP2/ERF genes; however, their functionality remains poorly understood. In this work, the 14th and 19th amino acid differences in the AP2 domain were used to distinguish DREB and ERF subfamily members. Even when the AP2 domain of 68 ERF proteins from 20 plant species and motifs in tomato DREB and ERF proteins were compared, the binding ability of DREB and ERF proteins with DRE/CRT and/or GCC boxes remained unknown. During fruit development and ripening, the expressions of 13 DREB and 19 ERF subfamily genes showed some regular changes, and the promoters of most genes had ARF, DRE/CRT, and/or GCC boxes. This suggests that these genes directly or indirectly respond to IAA and/or ethylene (ET) signals during fruit development and ripening. Moreover, some of these may feedback regulate IAA or ET biosynthesis. In addition, 16 EAR motif-containing ERF genes in tomato were expressed in many organs and their total transcripts per million (TPM) values exceeded those of other ERF genes in most organs. To determine whether the EAR motif in EAR motif-containing ERF proteins has repression function, their EAR motifs were retained or deleted in a yeast one-hybrid (YIH) assay. The results indicate that most of EAR motif-containing ERF proteins lost repression activity after deleting the EAR motif. Moreover, some of these were expressed during ripening. Thus, these EAR motif-containing ERF proteins play vital roles in balancing the regulatory functions of other ERF proteins by completing the DRE/CRT and/or GCC box sites of target genes to ensure normal growth and development in tomato.
Automatic Decision-Making Style Recognition Method Using Kinect Technology
Authors: Guo Yu , Liu Xiaoqian , Wang Xiaoyang , Zhu Tingshao , Zhan Wei
Published: March 2022

In recent years, somatosensory interaction technology, represented by Microsoft’s Kinect hardware platform, has been widely used in various fields, such as entertainment, education, and medicine. Kinect technology can easily capture and record behavioral data, which provides new opportunities for behavioral and psychological correlation analysis research. In this paper, an automatic decision-style recognition method is proposed. Experiments involving 240 subjects were conducted to obtain face data and individual decision-making style score. The face data was obtained using the Kinect camera, and the decision-style score were obtained via a questionnaire. To realize automatic recognition of an individual decision-making style, machine learning was employed to establish the mapping relationship between the face data and a scaled evaluation of the decision-making style score. This study adopts a variety of classical machine learning algorithms, including Linear regression, Support vector machine regression, Ridge regression, and Bayesian ridge regression. The experimental results show that the linear regression model returns the best results. The correlation coefficient between the linear regression model evaluation results and the scale evaluation results was 0.6, which represents a medium and higher correlation. The results verify the feasibility of automatic decision-making style recognition method based on facial analysis.
Influence of Public Health Services on the Goal of Ending Tuberculosis: Evidence From Panel Data in China
Authors: Chen Yang , Zhou Qingyu , Yang Xinmei , Shi Peiwu , Shen Qunhong , Zhang Zhaoyang , Chen Zheng , Pu Chuan , Xu Lingzhong , Hu Zhi , Ma Anning , Gong Zhaohui , Xu Tianqiang , Wang Panshi , Wang Hua , Hao Chao , Li Chengyue , Hao Mo
Published: March 2022

Background

The World Health Organization has proposed an initiative to “end tuberculosis (TB).” Unfortunately, TB continues to endanger the health of people worldwide. We investigated the impact of public health services (PHS) in China on TB incidence. In this way, we provided policy ideas for preventing the TB epidemic.

Methods

We used the “New Public Management Theory” to develop two indicators to quantify policy documents: multisector participation (MP) and the Assessable Public Health Service Coverage Rate (ASCR). The panel data from 31 provinces in Chinese mainland were collected from 2005 to 2019 based on 1,129 policy documents and the China Statistical Yearbook. A fixed-effect model was used to determine the impact of MP and the ASCR on TB incidence.

Results

From 2005 to 2019, the average MP increased from 89.25 to 97.70%, and the average ASCR increased from 53.97 to 78.40% in Chinese mainland. However, the development of ASCR between regions was not balanced, and the average level in the western region was lower than that in the eastern coastal provinces. With an increase in MP and the ASCR, the TB incidence had been decreasing gradually in recent years. The panel analysis results showed that MP (β = −0.76, p < 0.05). and ASCR (β = −0.40, p < 0.01) had a negative effect on TB incidence, respectively. Even if the control variables were added, the negative effects of MP (β = −0.86, p < 0.05) and ASCR (β = −0.35, p < 0.01) were still statistically significant.

Conclusions

Promoting the participation of multiple departments, as well as emphasizing the quality of PHS delivery, are important ways to alleviate the TB epidemic. The settings of evaluation indices for PHS provision should be strengthened in the future.
Laparoscopic Pancreaticoduodenectomy in Elderly Patients: Systematic Review and Meta-Analysis
Authors: Wang Qiang , Chen Chengxin , Li Haiyang
Published: March 2022

Background

The safety and efficacy of laparoscopic pancreaticoduodenectomy (LPD) in elderly patients who often suffer from pre-existing conditions (e.g., cardiovascular diseases) and poor functional reserve remain unclear. This meta-analysis aimed to evaluate the safety and efficacy of LPD in elderly patients.

Methods

A systematic literature search was conducted using the PubMed, Embase, Web of Science, and Cochrane Library databases. All studies published from their inception to January 2022 reporting perioperative outcomes after LPD in elderly patients were included in the search (Group 1, comparing the perioperative outcomes of LPD and OPD in elderly patients; Group 2, comparing the perioperative outcomes after LPD between elderly and non-elderly patients). The evaluated outcomes included perioperative mortality, postoperative complications, conversion, operative time, estimated blood loss (EBL), postoperative hospital stay (POHS), and readmission.

Results

In total 8 studies were included in the meta-analysis. Pooled analysis of Group 1 showed that EBL, 90-day mortality, major morbidity, bile leak, POH, abdominal infection, reoperation, POP, POCE, and readmission were not significantly different between the LPD and the OPD group. LPD was associated with longer operative time, lower POPF rate, lower DEG rate, and shorter POHS. Pooled analysis of Group 2 showed that mortality, major morbidity, POPF, DEG, bile leak, POH, abdominal infection, reoperation, conversion, operative time, EBL, and readmission were not significantly different between the elderly and the non-elderly group. The POHS of elderly group was significantly longer than non-elderly group.

Conclusion

LPD may be a safe and feasible procedure for elderly patients and is associated with short POHS.
Lung Biopsy With a Non-intubated VATS Approach in an Obese Population: Indications and Results
Authors: Cherchi Roberto , Ferrari Paolo Albino , Guerrera Francesco , Grimaldi Giulia , Pinna-Susnik Matteo , Murenu Alessandro , Rosboch Giulio Luca , Lybéris Paraskevas , Ibba Federica , Balsamo Ludovica , Saderi Laura , Fois Alessandro Giuseppe , Ruffini Enrico , Sotgiu Giovanni
Published: March 2022

Background

According to the international guidelines, patients affected by interstitial lung disease with unusual clinical presentation and radiological findings that are not classic for usual interstitial pneumonia end up meeting criteria for surgical lung biopsy, preferably performed with video-assisted thoracic surgery. The growing appeal of non-intubated thoracic surgery has shown the benefits in several different procedures, but the strict selection criteria of candidates are often considered a limitation to this approach. Although several authors define obesity as a contraindication for non-intubated thoracoscopic surgery, the assessment of obesity as a dominant risk factor represents a topic of debate when minor tubeless procedures such as lung biopsy are considered. Our study aims to investigate the impact of obesity on morbidity and mortality in non-intubated lung biopsy patients with interstitial lung disease, analyzing the efficacy and safeness of this procedure.

Materials and Methods

The study group of 40 obese patients consecutively collected from 202 patients who underwent non-intubated lung biopsy was compared with overweight and normal-weight patients, according to their body mass index. Post-operative complications were identified as the primary endpoint. The other outcomes explored were the early 30-day mortality rate and intraoperative complications, length of surgery, post-operative hospitalization, patient's pain feedback, and diagnostic yield.

Results

The overall median age of the patients was 67.4 years (60, 73.5). No 30-day mortality or significant differences in terms of post-operative complications (P = 0.93) were noted between the groups. The length of the surgery was moderately longer in the group of obese patients (P = 0.02). The post-operative pain rating scale was comparable among the three groups (P = 0.45), as well as the post-operative length of stay (P = 0.96). The diagnosis was achieved in 99% of patients without significant difference between groups (P = 0.38).

Conclusion

Our analysis showed the safety and efficacy of surgical lung biopsy with a non-intubated approach in patients affected by lung interstitiopathy. In the context of perioperative risk stratification, obesity would not seem to affect the morbidity compared to normal-weight and overweight patients undergoing this kind of diagnostic surgical procedure.
Using Systemic Inflammatory Markers to Predict Microvascular Invasion Before Surgery in Patients With Hepatocellular Carcinoma
Authors: Li Shumin , Zeng Qianwen , Liang Ruiming , Long Jianyan , Liu Yihao , Xiao Han , Sun Kaiyu
Published: March 2022

Background

Mounting studies reveal the relationship between inflammatory markers and post-therapy prognosis. Yet, the role of the systemic inflammatory indices in preoperative microvascular invasion (MVI) prediction for hepatocellular carcinoma (HCC) remains unclear.

Patients and Methods

In this study, data of 1,058 cases of patients with HCC treated in the First Affiliated Hospital of Sun Yat-sen University from February 2002 to May 2018 were collected. Inflammatory factors related to MVI diagnosis in patients with HCC were selected by least absolute shrinkage and selection operator (LASSO) regression analysis and were integrated into an “Inflammatory Score.” A prognostic nomogram model was established by combining the inflammatory score and other independent factors determined by multivariate logistic regression analysis. The receiver operating characteristic (ROC) curve and the area under the curve (AUC) were used to evaluate the predictive efficacy of the model.

Results

Sixteen inflammatory factors, including neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, etc., were selected by LASSO regression analysis to establish an inflammatory score. Multivariate logistic regression analysis showed that inflammatory score (OR = 2.14, 95% CI: 1.63–2.88, p < 0.001), alpha fetoprotein (OR = 2.02, 95% CI: 1.46–2.82, p < 0.001), and tumor size (OR = 2.37, 95% CI: 1.70–3.30, p < 0.001) were independent factors for MVI. These three factors were then used to establish a nomogram for MVI prediction. The AUC for the training and validation group was 0.72 (95% CI: 0.68–0.76) and 0.72 (95% CI: 0.66–0.78), respectively.

Conclusion

These findings indicated that the model drawn in this study has a high predictive value which is capable to assist the diagnosis of MVI in patients with HCC.
The Evolving Role of Triage and Appointment-Based Admission to Improve Service, Care and Outcomes in Animal Shelters
Authors: Hurley Kate F.
Published: March 2022

The historical norm for many animal shelters has been to admit animals on an unscheduled basis, without prior assessment of animal or client need or regard to the shelter's ability to deliver humane care or ensure appropriate outcomes. This approach allows little opportunity to provide finders or owners with alternatives to keep pets safe in their homes or community rather than being impounded. In addition to needlessly impounding animals and separating pets from families, unmanaged/unscheduled admission frequently results in animal influx exceeding shelter capacity, leading to crowding, stress, disease, and euthanasia of animals, as well as poor customer experience, compromised staffing efficiency and decreased organizational effectiveness. Many of these harmful consequences disproportionately impact vulnerable community members and their pets. Triage and appointment-based services have been well developed in healthcare and other service sectors allowing organizations to prioritize the most urgent cases, align services with organizational resources and provide situation-specific solutions that may include virtual support or referral as appropriate. This article discusses the trend in animal sheltering toward triage and appointment-based services that parallels the use of these practices in human healthcare. Reported positive results of this approach are detailed including improved staff morale, reduced disease rates and substantially reduced euthanasia. These positive outcomes support the endorsement of triage and appointment-based services by multiple North American animal welfare professional and academic organizations, recognizing that it better realizes the goals of shelters to serve the common good of animals and people in the most humane, equitable and effective possible way.
Comprehensive Review on Climate Control and Cooling Systems in Greenhouses under Hot and Arid Conditions
Authors: Soussi Meriem , Chaibi Mohamed Thameur , Buchholz Martin , Saghrouni Zahia
Published: March 2022

This work is motivated by the difficulty of cultivating crops in horticulture greenhouses under hot and arid climate conditions. The main challenge is to provide a suitable greenhouse indoor environment, with sufficiently low costs and low environmental impacts. The climate control inside the greenhouse constitutes an efficient methodology for maintaining a satisfactory environment that fulfills the requirements of high-yield crops and reduced energy and water resource consumption. In hot climates, the cooling systems, which are assisted by an effective control technique, constitute a suitable path for maintaining an appropriate climate inside the greenhouse, where the required temperature and humidity distribution is maintained. Nevertheless, most of the commonly used systems are either highly energy or water consuming. Hence, the main objective of this work is to provide a detailed review of the research studies that have been carried out during the last few years, with a specific focus on the technologies that allow for the enhancement of the system effectiveness under hot and arid conditions, and that decrease the energy and water consumption. Climate control processes in the greenhouse by means of manual and smart control systems are investigated first. Subsequently, the different cooling technologies that provide the required ranges of temperature and humidity inside the greenhouse are detailed, namely, the systems using heat exchangers, ventilation, evaporation, and desiccants. Finally, the recommended energy-efficient approaches of the desiccant dehumidification systems for greenhouse farming are pointed out, and the future trends in cooling systems, which include water recovery using the method of combined evaporation–condensation, as well as the opportunities for further research and development, are identified as a contribution to future research work.
Synchrotron Radiation Circular Dichroism, a New Tool to Probe Interactions between Nucleic Acids Involved in the Control of ColE1-Type Plasmid Replication
Authors: Wien Frank , Kubiak Krzysztof , Turbant Florian , Mosca Kevin , Węgrzyn Grzegorz , Arluison Véronique
Published: March 2022

Hfq is a bacterial master regulator which promotes the pairing of nucleic acids. Due to the high molecular weight of the complexes formed between nucleic acids and the amyloid form of the protein, it is difficult to analyze solely by a gel shift assay the complexes formed, as they all migrate at the same position in the gel. In addition, precise kinetics measurements are not possible using a gel shift assay. Here, we used a synchrotron-based biophysical approach, synchrotron radiation circular dichroism (SRCD), to probe the interaction of the Escherichia coli Hfq C-terminal amyloid region with nucleic acids involved in the control of ColE1-like plasmid replication. We observed that this C-terminal region of Hfq has an unexpected and significant effect on the annealing of nucleic acids involved in this process and, more importantly, on their alignment. Functional consequences of this newly discovered property of the Hfq amyloid region are discussed in terms of the biological significance of Hfq in the ColE1-type plasmid replication process and antibiotic resistance.
Influence of Super Absorbent Polymer on Root Characteristics and Anchorage of Amorpha fruticosa on Rocky Slope
Authors: Hou Shujun , Sun Hailong , Zhou Yinghua
Published: March 2022

Super absorbent polymer (SAP), known as a water retention agent, has a high capacity for water absorption, which can help enhance the soil structure. This paper studied the effects of SAP dosages on the root characteristics and anchorage of Amorpha fruticosa on rock slopes. The internal relationship between root growth effect and soil was discussed, and a specific reference was provided for the rational application of SAP on slopes. Using the pull-out and tensile resistance tests, we systematically studied the changes of soil properties, root distribution, root tensile strength, and root anchorage under six different SAP dosages. The results indicated that: (1) With the increase in SAP dosage, the natural soil water content and water content after 24 h of watering increased significantly, whereas the contents of TN, TP, and TK decreased dramatically. (2) With the increase in SAP dosage, the amount and length of first-order and secondary lateral roots decreased significantly, and there was no significant difference in diameter. The amount of downslope first-order and unembedded secondary lateral roots is greater than upslope. The amount of upslope embedded secondary lateral roots is greater than in downslope. (3) Tensile strength: embedded secondary root > non-embedded secondary root > first-order lateral root; upslope root > downslope root. (4) With the increase in SAP dosage, the plant anchorage drops noticeably. This study concluded that the significant addition of SAP could enhance the tensile strength of upslope embedded secondary lateral roots but would adversely affect soil nutrients, root distribution, and root anchorage. The addition of SAP in this test had no significant effect on improving slope stability. From the perspective of reinforcement capacity, we cannot blindly pursue the survival rate and other high dosage use of water retention agents to increase the risk of slope destabilization.
Protons Interaction with Nomex Target: Secondary Radiation from a Monte Carlo Simulation with Geant4
Authors: Loffredo Filomena , Vardaci Emanuele , Bianco Davide , Di Nitto Antonio , Quarto Maria
Published: March 2022

The study of suitable materials to shield astronauts from Galactic Cosmic Rays (GCR) is a topic of fundamental importance. The choice of the material must take into account both the secondary radiation produced by the interaction between primary radiation and material and its shielding ability. The physics case presented here deals with the interaction of a proton beam with a Nomex shield, namely, a target material with a mass thickness of 20 g cm−2. The study was conducted with the simulation code DOSE based on the well-known simulation package Geant4. This article shows the properties of secondary radiations produced in the target by the interaction of a proton beam in an energy range characterizing the GCR spectrum. We observed the production of ions of masses and charges lower than the chemical elements that make up Nomex, and also a significant production of neutrons, protons, and 𝛼 particles.
Eco-Geophysical and Geoecological Factors in Assessing the State of the Geological Environment Based on the Analysis of Spatial Databases of the Territory of the Republic of North Ossetia–Alania
Authors: Zaalishvili Vladislav , Burdzieva Olga , Kanukov Aleksandr , Zaks Tamaz
Published: March 2022

The article considers the main sources of pollutionin the territory of the Republic of North Ossetia–Alania. A study of environmental geophysical factors in the city of Vladikavkaz was carried out at 126 points; indicators of noise pollution, electric fields and the level of gamma radiation were measured. A geoaccumulation index of heavy metals in soils and indices of carcinogenic and non-carcinogenic risks were calculated and corresponding maps were constructed. The obtained data supporting a high level of carcinogenic risk are consistent with a high level of cancer morbidity in the city, which indicates a close relationship between morbidity and the carcinogenic risk index. It has been determined that emissions from road transport are greater by an order of magnitude than stationary sources emissions, while there is a steady trend towards an increase in air pollution as a result of the increasing negative impact of motor vehicle emissions. It has been established that the most hazardous way for heavy metals to enter the human body from the soil is by inhalation. It has been determined that in areas where environmental pollution with heavy metals is higher, cancer morbidity is also higher.
The Average Eccentricity of Block Graphs: A Block Order Sequence Perspective
Authors: Li Xingfu , Yu Guihai , Das Kinkar Chandra
Published: March 2022

A graph is a block graph if its blocks are all cliques. In this paper, we study the average eccentricity of block graphs from the perspective of block order sequences. An equivalence relation is established under the block order sequence and used to prove the lower and upper bounds of the eccentricity on block graphs. The result is that the lower and upper bounds of the average eccentricity on block graphs are 1 and 1n⌊34n2−12n⌋, respectively, where n is the order of the block graph. Finally, we devise a linear time algorithm to calculate the block order sequence.
Infigratinib (BGJ 398), a Pan-FGFR Inhibitor, Targets P-Glycoprotein and Increases Chemotherapeutic-Induced Mortality of Multidrug-Resistant Tumor Cells
Authors: Boichuk Sergei , Dunaev Pavel , Mustafin Ilshat , Mani Shinjit , Syuzov Kirill , Valeeva Elena , Bikinieva Firuza , Galembikova Aigul
Published: March 2022

The microtubule-targeting agents (MTAs) are well-known chemotherapeutic agents commonly used for therapy of a broad spectrum of human malignancies, exhibiting epithelial origin, including breast, lung, and prostate cancer. Despite the impressive response rates shortly after initiation of MTA-based therapy, the vast majority of human malignancies develop resistance to MTAs due to the different mechanisms. Here, we report that infigratinib (BGJ 398), a potent FGFR1-4 inhibitor, restores sensitivity of a broad spectrum of ABCB1-overexpressing cancer cells to certain chemotherapeutic agents, including paclitaxel (PTX) and doxorubicin (Dox). This was evidenced for the triple-negative breast cancer (TNBC), and gastrointestinal stromal tumor (GIST) cell lines, as well. Indeed, when MDR-overexpressing cancer cells were treated with a combination of BGJ 398 and PTX (or Dox), we observed a significant increase of apoptosis which was evidenced by an increased expression of cleaved forms of PARP, caspase-3, and increased numbers of Annexin V-positive cells, as well. Moreover, BGJ 398 used in combination with PTX significantly decreased the viability and proliferation of the resistant cancer cells. As expected, no apoptosis was found in ABCB1-overexpressing cancer cells treated with PTX, Dox, or BGJ 398 alone. Inhibition of FGFR-signaling by BGJ 398 was evidenced by the decreased expression of phosphorylated (i.e., activated) forms of FGFR and FRS-2, a well-known adaptor protein of FGFR signaling, and downstream signaling molecules (e.g., STAT-1, -3, and S6). In contrast, expression of MDR-related ABC-transporters did not change after BGJ 398 treatment, thereby suggesting an impaired function of MDR-related ABC-transporters. By using the fluorescent-labeled chemotherapeutic agent PTX-Alexa488 (Flutax-2) and doxorubicin, exhibiting an intrinsic fluorescence, we found that BGJ 398 substantially impairs their efflux from MDR-overexpressing TNBC cells. Moreover, the efflux of Calcein AM, a well-known substrate for ABCB1, was also significantly impaired in BGJ 398-treated cancer cells, thereby suggesting the ABCB1 as a novel molecular target for BGJ 398. Of note, PD 173074, a potent FGFR1 and VEGFR2 inhibitor failed to retain chemotherapeutic agents inside ABCB1-overexpressing cells. This was consistent with the inability of PD 173074 to sensitize Tx-R cancer cells to PTX and Dox. Collectively, we show here for the first time that BGJ 398 reverses the sensitivity of MDR-overexpressing cancer cells to certain chemotherapeutic agents due to inhibition of their efflux from cancer cells via ABCB1-mediated mechanism.
The Minimal Residual Disease Using Liquid Biopsies in Hematological Malignancies
Authors: Colmenares Rafael , Álvarez Noemí , Barrio Santiago , Martínez-López Joaquín , Ayala Rosa
Published: March 2022

The study of cell-free DNA (cfDNA) and other peripheral blood components (known as “liquid biopsies”) is promising, and has been investigated especially in solid tumors. Nevertheless, it is increasingly showing a greater utility in the diagnosis, prognosis, and response to treatment of hematological malignancies; in the future, it could prevent invasive techniques, such as bone marrow (BM) biopsies. Most of the studies about this topic have focused on B-cell lymphoid malignancies; some of them have shown that cfDNA can be used as a novel way for the diagnosis and minimal residual monitoring of B-cell lymphomas, using techniques such as next-generation sequencing (NGS). In myelodysplastic syndromes, multiple myeloma, or chronic lymphocytic leukemia, liquid biopsies may allow for an interesting genomic representation of the tumor clones affecting different lesions (spatial heterogeneity). In acute leukemias, it can be helpful in the monitoring of the early treatment response and the prediction of treatment failure. In chronic lymphocytic leukemia, the evaluation of cfDNA permits the definition of clonal evolution and drug resistance in real time. However, there are limitations, such as the difficulty in obtaining sufficient circulating tumor DNA for achieving a high sensitivity to assess the minimal residual disease, or the lack of standardization of the method, and clinical studies, to confirm its prognostic impact. This review focuses on the clinical applications of cfDNA on the minimal residual disease in hematological malignancies.