bluuebunny/crossref_metadata_2025 huggingface dataset:
THE PROBLEM OF SEARCHING THE MEANING OF HUMAN EXISTENCE: CONTEMPORARY CONTEXT
Authors: V. M. Petrushov  , V. M. Shapoval
Published: August 2020

Purpose. The purpose of the article is the analysis of the reasons and grounds of the crisis in the sphere of meaning-making, as well as searching answers to the questions about the meaning of human life in the contemporary world, which are maximally relevant in connection with the escalation of global problems, revealing the points of convergence between various theoretical positions, evaluation of their heuristic potential. Theoretical basis of the research is the historical-philosophical, comparative and system approaches, as well as the analysis of philosophical insights in the field of global studies. Originality. Originality lies in the fact that this article is the first attempt to conduct comprehensive analysis in the problem of the sense of the Existence as it is presented in the first quarter of the 21st century and to relate it with the modern social situation that is characterized by a complex range of interconnected and interdependent anthropological problems of our time. Authors emphasize that the main reason in the crisis of meaning is that a man has lost touch with his roots, which is wildlife. He has created an artificial structure, civilization to satisfy his needs and finds no way to the transcendental, which is the true House of his being. Conclusions. A human must refuse from false self-conceit concerning his potential omniscience and omnipotence, cease dictating his own rules to the Existence, determine the boundaries of his freedom and try to clearly realize his place in the objective structure of being. The global situation can change for the better only if a dramatic change in the area of meaning-making happens. The decisive force, which may encourage nudging to the positive changes, can be either the free will of people who have realized the criticality of the situation or external natural and social circumstances that will make people reorganize radically. The proper prioritizing, a deep awareness of universal goals and solidarity between people could be the value basis that will become the foundation to find the meaning and create a more favorable future.
CARTESIAN PERSONAL METAPHYSICS
Authors: Malivskyi A. M.
Published: August 2020

Purpose. To consider the personal nature of Cartesian metaphysics. Its implementation involves: a) outlining methodological changes in the philosophy of the twentieth century; b) analysis of ways to interpret anthropological component of philosophizing in Descartes studies; c) appeal to Descartes’ texts to clarify the authentic form of his interpretation of metaphysics. Theoretical basis. I base my view of Descartes’ legacy on the conceptual positions of phenomenology, existentialism and hermeneutics. Originality. Based on Descartes’ own concept of teaching, the author substantiates the personal nature of Cartesian metaphysics. Important prerequisites for its comprehension are attention to the ethical motive as the driving force of philosophizing and recognition of the importance of the poetic worldview. The idea of the basic role of poetics finds its meaningful confirmation in the texts of the philosopher, who interprets the main areas of philosophy (science, morality and medicine) as the forms of art. Conclusions. Based on his own vision of anthropology and metaphysics as the forms of completion of the revolution initiated by Copernicus, the author defends the idea of the constitutive presence of personality in Descartes’ metaphysics. In the process of studying the research literature, methodological guidelines are outlined in the form of the importance of personal determination of the search for truth, the key role of ethical motive and art as components of philosophizing. The thesis about the poetic form of presentation of metaphysics by Descartes as a form of fixation of its personal dimension is substantiated.
Um jogo de tabuleiro utilizando tópicos contextualizados em Física
Authors: Benedetti Filho Edemar , Silva Adriana de Oliveira Delgado , Favaretto Danilo Vieira
Published: August 2020

Resumo Este trabalho apresenta a elaboração e aplicação de um jogo de tabuleiro didático, como uma atividade de revisão, envolvendo conceitos de física relacionados ao cotidiano dos alunos. A pesquisa foi aplicada para 51 estudantes do terceiro ano do ensino médio, no final do primeiro semestre. A metodologia destaca a importância de se empregar o lúdico na exploração das habilidades de argumentação e na relação social dos estudantes que, normalmente, não são contempladas em uma aula tradicional. Observou-se um progresso nas relações entre os próprios alunos e também entre alunos-professor, durante a elaboração conjunta das respostas às perguntas contidas nas cartas do jogo. Cabe destacar a importância destas interações nas discussões teóricas dos conteúdos de física e na construção das relações entre esses conceitos e o seu dia-a-dia. Após a aplicação da atividade, o professor descreveu que houve um maior interesse dos estudantes pela disciplina de física, além de uma postura mais crítica, observada pelos diálogos em sala de aula, durante a finalização da disciplina.
Psicologia moral e economia solidária: relações teóricas
Authors: Borges Thelma Pontes , Souza Maria Thereza Costa Coelho de
Published: August 2020

Resumo A psicologia moral é focada na compreensão de como características morais se desenvolvem e como são integradas à personalidade humana. Consideramos que o indivíduo que possua aspectos relativos aos deveres e às virtudes desenvolve uma personalidade ética. Por sua vez, a economia solidária traduz em suas práticas aspectos de comportamento ético, como preço justo, cooperação e sustentabilidade. Nosso trabalho tem o propósito de apresentar a psicologia moral como instrumental teórico capaz de favorecer os debates acerca da economia solidária, demonstrando que ambas trazem em seus princípios a formação humana, a ética nas relações e o desenvolvimento individual e comunitário. Assim, conhecer o debate sobre a psicologia moral é condição para discutir projetos de intervenção que visem à sensibilização e à formação para economia solidária.
Análise crítico-emancipatória de discurso: um ensaio metodológico
Authors: Lara Luiz Gustavo Alves de , Vizeu Fabio
Published: August 2020

Resumo Neste ensaio metodológico propomos uma abordagem crítico-emancipatória para análise de discurso (AC-ED) sob os contributos da hermenêutica e da epistemologia lacaniana. Consideramos que esse tipo de análise se trata de um proceder interpretativo epistemologicamente orientado, em relação ao qual a discussão sobre protocolos procedimentalistas não é central. Pelo aparato meta-analítico da AC-ED, argumentamos em favor da virtude da consciência do(a) pesquisador(a) sobre o próprio discurso científico, o que lhe torna mais vigilante em relação a sua postura analítica e, consequentemente, sua postura emancipatória em relação à realidade. Desenvolvemos nosso argumento apreciando a polissemia do termo emancipação, exemplificada a partir diferentes paradigmas versados em análises críticas de discurso: da produção, da comunicação e da linguagem. Construída a partir do paradigma da linguagem, sinalizamos o potencial da AC-ED como uma proposta meta-reflexiva para desenvolvimento de análise de discurso no campo social.
Historia e investigación social cualitativa: reflexiones en torno de la historia comparada y la historia de vida
Authors: Granato Leonardo , Lopes Fernanda Tarabal , Costa Alessandra de Sá Mello da
Published: August 2020

Resumen Este ensayo teórico tiene el objetivo de reflexionar, desde una perspectiva brasileña, sobre el uso del método histórico en las ciencias sociales en general, y en las áreas de los estudios organizacionales y de la administración pública en particular. Para ello, con base en una revisión bibliográfica, se eligió profundizar la reflexión a partir de los métodos histórico-comparativos y de la historia de vida, dentro del paradigma cualitativo, con el fin de ofrecer una perspectiva más especializada sobre las implicancias de tales métodos para las subdisciplinas antes mencionadas. Se concluye que el método histórico en general, así como el histórico-comparativo y la historia de vida en particular, promueven nuevas formas de comprensión de la realidad, que conectan lo singular con lo global, lo objetivo con lo subjetivo, lo pasado con lo contemporáneo, generando perspectivas innovadoras para el análisis social, organizacional y político.
Relações raciais e histórias de vida: trabalhadores industriais negros em foco
Authors: Silva Matheus Arcelo Fernandes , Saraiva Luiz Alex Silva
Published: August 2020

Resumo A construção das identidades negras no Brasil é marcada por uma série de lutas que influenciaram diretamente os avanços institucionais recentes em relação à temática, além de contribuírem para a desconstrução do mito da democracia racial. O objetivo deste artigo é compreender e analisar como se apresentam elementos relacionados às relações raciais nas histórias de vida de trabalhadores industriais negros. Para isso, é empreendido um debate sobre elementos que marcam a construção das identidades negras no Brasil, bem como sobre a ideia de democracia racial. A partir do método indutivo, os dados foram produzidos com base em histórias de vida, material trabalhado mediante a análise estruturalista do discurso, em especial no que diz respeito aos elementos sócio-históricos que permeiam os discursos. Os dados apresentam as relações raciais nas histórias de Leila e Clóvis, sujeitos da pesquisa, sendo a primeira marcada por uma posição política quanto à miscigenação, e o segundo por ter sido vítima de racismo. As principais conclusões sugerem que, mesmo com os avanços institucionais proporcionados pelos movimentos negros, conscientizar-se da sua negritude é o primeiro passo para que as pessoas negras possam ocupar, efetivamente, qualquer lugar que desejem na sociedade.
Non-adherence to hemodialysis, perception of the illness, and severity of advanced nephropathy
Authors: Dantas Lianna Gonçalves , Rocha Mario Seixas , Cruz Constança Margarida Sampaio
Published: August 2020

Abstract Introduction: Hemodialysis (HD) is a complex therapy that imposes several changes in the patient's life. Failure to follow therapy recommendations is called non-adherence (NA). The patient's illness perception, severity of chronic kidney disease, and individual strategies for coping with HD can have an impact on NA to the demands of therapy. Methods: This was a cross-sectional study with end-stage renal disease patients on conventional HD in Salvador, Bahia. We evaluated attendance to treatment and interdialytic weight gain (IDWG) as parameters of NA to HD, and investigated its association with clinical aspects and measures of disease perception (illness effects questionnaire - IEQ) and severity of nephropathy (end stage renal disease severity index - ESRD-SI), by analyzing Pearson or Spearman correlation. Results: 79 patients were evaluated, 57% male, aged 53.1 ± 12.3 years, with length of HD of 108 (89 - 131.5) months. Age correlated with ESRD-SI (r = 0.43) and NA parameters: negative correlation with relative IDWG (r = -0.41) and reduction in sessions (r = -0.31) and positive correlation with %HD performed (r = 0.25). The scores on the IEQ and ESRD-SI showed a positive correlation (r = 0.44; p <0.001), but did not show any correlation with the analyzed NA parameters. Conclusions: We did not find a correlation between illness perception and severity index of advanced nephropathy with the behaviors of NA to chronic HD. In this study, age correlated both with the perception of severity of advanced nephropathy and the parameters of NA to chronic HD.
Desenvolvimento de competências na perspectiva de docentes de ensino superior: estudo em representações sociais
Authors: Polonia Ana da Costa , Santos Maria de Fátima Souza
Published: August 2020

Resumo O propósito desta pesquisa é identificar o desenvolvimento de competências acadêmicas no ensino superior, na perspectiva dos docentes, contando com o aporte da teoria das representações sociais. Para coleta de informações foi aplicado o instrumento denominado de Técnica de Associação Livre de Palavras, de caráter projetivo, a 154 professores de ensino superior de três instituições privadas do Distrito Federal. A análise prototípica foi produto do software IRaMuteQ, que analisou as palavras evocadas pelos professores quando empregada a expressão indutora desenvolver competências acadêmica é... O núcleo central foi composto pelas seguintes palavras: formação, habilidade, reflexão, fundamental, responsabilidade e desenvolvimento. Já nos elementos de contraste emergiram os termos cientificidade, ética, autonomia, concentrar, qualificação e planejamento. Esses dados apontam o fomento ao processo de aprendizagem embasado na concepção ativa e interativa do processo, tendo o discente como protagonista. Os dados evidenciam a construção de conhecimentos, apropriação de diferentes saberes, mobilização do fazer e ainda a articulação teórico-prática dentro de uma perspectiva humanizadora, ética e estética. Ainda, pode-se reconhecer que o desenvolvimento de competências não se restringe à dimensão operatória, mas envolve os universos tecnológico, metodológico, didático, relacional e vinculado à esfera do trabalho, além de serem indissociáveis os aspectos social, cultural e do fazer profissional. É um projeto profissional docente que assume uma visão de sociedade, em que o processo ético e a dimensão política, metodológica, operacional, técnica se interpenetram e encontram-se transversalizadas pela perspectiva humanista, de forma que compromisso, responsabilidade e reflexão são demandas constantes frente às transformações no mundo do trabalho e problemáticas do âmbito social.
A reinvenção do cotidiano em tempos de pandemia
Authors: Guizzo Bianca Salazar , Marcello Fabiana de Amorim , Müller Fernanda
Published: August 2020

Resumo Em 2020, o Estatuto da Criança e do Adolescente (ECA) faz 30 anos. Esse marco coincide com a pandemia causada pela COVID-19, que coloca em conflito noções de direito ligadas à preservação da vida e da liberdade apresentadas pelo próprio ECA. Diante desse contexto, este artigo trata a respeito de reinvenções e deslocamentos diante da quarentena, os quais se pautam pelo direito à vida ao mesmo tempo em que restringem o direito à liberdade. Ao assumir a inseparabilidade que Michel de Certeau postula quanto às noções de estratégia e tática, o artigo explora, metodologicamente, seis cenas expressivas da reinvenção do cotidiano – e que estão vinculadas, cada uma a seu modo, a três dimensões específicas: a relação de famílias com as tecnologias; a relação das famílias com a escola; e a relação das famílias com suas crianças. As quatro cenas iniciais, exploradas a partir da configuração de estratégia, indicam esforços para a conformação das formas de organização do social. Neste caso, fica evidente como, de um lado, a relação das famílias com as tecnologias, e, de outro, a relação das famílias com a escola sugerem práticas comprometidas com a manutenção de formas de existir, mesmo em meio a um contexto de excepcionalidade. As duas últimas cenas, situadas a partir da configuração de tática, dão visibilidade às práticas cotidianas das famílias com suas crianças, ou seja, ao que entendemos serem relatos de pessoas ordinárias e que, justamente por meio dessa condição, apontam possibilidades de criação do novo.
Detection of tick-borne rickettsial pathogens in naturally infected dogs and dog-associated ticks in Medellin, Colombia
Authors: Arroyave Esteban , Cornwell Emily Rose , McBride Jere Williams , Díaz Carlos Arley , Labruna Marcelo Bahia , Rodas Juan David
Published: August 2020

Abstract Tick-borne rickettsial pathogens (TBRP) are important causes of infections in both dogs and humans. Dogs play an important role as a biological host for several tick species and can serve as sentinels for rickettsial infections. Our aim was to determine the presence of TBRP in dogs and in dog-associated ticks and their potential risk to human diseases in Medellin, Colombia. DNA for E. canis (16S rRNA and dsb) and A. platys (groEl) was detected in 17.6% (53/300) and 2.6% (8/300) of dogs, respectively. Antibodies against Ehrlichia spp. 82 (27.3%) and Anaplasma spp. 8 (2.6%) were detected in dogs. Antibody reactivity against both agents were found in 16 dogs (5.3%). Eight dogs showed antibody for Rickettsia spp. with titers that suggest 3 of them had a probable exposure to R. parkeri. Rhipicephalus sanguineus s.l. (178/193) was the main tick in dogs, followed by R. microplus (15/193). The minimum infection rates (MIR) in R. sanguineus were 11.8% for E. canis and 3.4% for A. platys. E. canis and A. platys are the main TBRP infecting dogs and ticks and R. sanguineus s.l. is likely involved in the transmission of both agents. Interestingly, we found serological evidence of exposure in dogs for spotted fever group rickettsiae.
Unusual feline Dirofilaria immitis infection: a case report
Authors: Alberigi Bruno , Oliveira Alexandre Calmon de , Vieira Grazielle Silva Rodrigues , Fernandes Priscila do Amaral , Labarthe Norma , Mendes-de-Almeida Flavya
Published: August 2020

Abstract Cats are less susceptible to Dirofilaria immitis infection than dogs. Although rare, the feline disease can be fatal even with low parasitic loads. The infection is often asymptomatic or has non-specific symptoms that are mainly associated with the death of immature worms. Microfilaremia is rare and transient. Normally, microfilaremia, when present, lasts for not more than 33 days. This study describes a feline case presenting with non-specific clinical signs and prolonged microfilaremia. Case: a random bred cat infected by feline leukemia virus (FeLV) that was found to be microfilaremic by chance. The infection was detected by the presence of microfilariae in a blood smear and was confirmed by antigen test (SNAP Feline Triple Test, Idexx®) and echocardiogram.
Subgoaling Techniques for Satisficing and Optimal Numeric Planning
Authors: Scala Enrico , Haslum Patrik , Thiébaux Sylvie , Ramirez Miquel
Published: August 2020

This paper studies novel subgoaling relaxations for automated planning with propositional and numeric state variables. Subgoaling relaxations address one source of complexity of the planning problem: the requirement to satisfy conditions simultaneously. The core idea is to relax this requirement by recursively decomposing conditions into atomic subgoals that are considered in isolation. Such relaxations are typically used for pruning, or as the basis for computing admissible or inadmissible heuristic estimates to guide optimal or satis_cing heuristic search planners. In the last decade or so, the subgoaling principle has underpinned the design of an abundance of relaxation-based heuristics whose formulations have greatly extended the reach of classical planning. This paper extends subgoaling relaxations to support numeric state variables and numeric conditions. We provide both theoretical and practical results, with the aim of reaching a good trade-o_ between accuracy and computation costs within a heuristic state-space search planner. Our experimental results validate the theoretical assumptions, and indicate that subgoaling substantially improves on the state of the art in optimal and satisficing numeric planning via forward state-space search.
Characterization and utilization of coal fly ash: a review
Authors: Panda Laxmidhar , Dash Subhakanta
Published: August 2020

Fly ash is a by-product, obtained mainly from thermal power plants, composed of silicon dioxide (SiO2) and aluminum oxide (Al2O3). Other oxides present in fly ash include iron (III) oxide (Fe2O3), calcium oxide (CaO), magnesium oxide (MgO) and so on. It may also contain some trace elements such as arsenic (As), zinc (Zn), lead (Pb), selenium (Se), gallium (Ga) and germanium (Ge) and some lesser amount of rare-earth elements. The particle size of fly ash normally ranges from 0.01 to 200 μm. Such variability in the particle size distribution of fly ash could influence the modification of fly ash into many useful products such as zeolites, geopolymers and other composite materials. The physical, chemical and mineralogical characteristics of fly ash combined with its easy availability make this an attractive raw material for various applications. In order to explore the potential use of fly ash, it is essential to look deeply into its characteristic properties. This review meticulously studies the characterization of fly ash by different modern techniques, including X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, thermogravimetric test, energy-dispersive X-ray spectroscopy, differential scanning calorimetry, secondary ion mass spectroscopy and Raman spectroscopy and attempts to correlate the same with its potential applications.
MEVKİLERİNE GÖRE AMATÖR FUTBOLCULARDA SEÇİLMİŞ PERFORMANS PARAMETRELERİ İLE ÇEVİKLİK ARASINDAKİ İLİŞKİ
Authors: AKTAS Samet , UÇAR Uğur Mehmet , KAPLAN Turgut
Published: August 2020

Bu araştırmanın amacı aktif olarak müsabaka döneminde futbol oynayan amatör futbolcularda mevkilerine göre, seçilmiş parametreler ile çeviklik arasındaki ilişkiyi ortaya koymaktır. Dört farklı amatör futbol takımından haftada en az 4 antrenman yapan her mevkiden 8’er futbolcu olmak üzere toplam 32 sporcu araştırmaya dahil edilmiştir. Araştırmaya katılan futbolcuların yaş ortalamaları 23,25±2,95 yıl, boy ortalamaları 179,63±3,63 cm, vücut ağırlığı ortalamaları ise 72,28±4,47 kg olarak bulunmuştur. Seçilmiş parametreler, Anaerobik Güç (AnG(kg.m/sn), Dikey Sıçrama, 10m,20m,30m sürat testi ve 505 çeviklik testi ile sınırlı tutulmuştur. Elde edilen verilerin analizinde SPSS 23 paket program kullanılmıştır, her bir parametreye ait veriler ortalama ve standart sapma olarak verilmiştir, seçilen parametrelerin çeviklik ile ilişkisinde ise Pearson Korelasyon testi kullanılmıştır. Bulgular değerlendirildiğinde 10 m sürat testi değerlerinde forvetlerin en yüksek ortalamaya sahip oldukları, bunu kaleciler ve defans oyuncularının takip ettiği görülmektedir, en düşük ortalamanın ise orta saha oyuncularına ait olduğu görülmüştür. Bu tespitin, kaleci ve forvet oyuncularının pozisyonları gereği algısal farklılık, antrenman durumu ve bireysel farklılıklardan kaynaklandığı düşünülmektedir (x2=9,73; p=0,021). Dikey sıçrama ile çeviklik arasındaki ilişkide bütün mevkilerde negatif yönde istatistiksek olarak anlamlı olmayan bir ilişki saptanmıştır. Sporcuların dikey sıçrama mesafesi arttıkça çeviklik becerisi de artmaktadır, bu da futbolun oyun içindeki aksiyonlarının çeviklik gerektirdiğini ve sıçrama yetisinin çevikliği etkilediğini destekleyebilir. Futbolun temel özelliklerinin içinde ani duruşlar ve yön değiştirme gibi çeviklik gerektiren aksiyonlarının olması çevikliğin futbolcularda ne kadar önemli olduğunu ortaya koymaktadır. Bu bağlamda çalışmadan çıkan sonuçlar doğrultusunda çeviklik antrenmanlarının yanında sürat ve anaerobik güç geliştirici antrenmanların yapılması futbolcuların müsabaka performansını da artıracağı düşünülmektedir.
Purification of anti-anti-SpA antibody (Ab-2) using SpA-bearing Staphylococcus aureus cells. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

A novel method was designed for the purification of Ab-2 from undiluted WSF of hyperimmune eggs. A total of six preparations were made.
Assembly of plasmids for LacI genotype-phenotype landscape measurement v1
Authors: Drew S Tack not provided , Alperovich Nina , Olga Vasilyeva not provided , Ross David
Published: August 2020

Here, we describe protocols for assembly of two different plasmids used for the measurement of the genotype-phenotype landscape of the LacI protein in E. coli: a Library Plasmid (pTY1, Figure 1a) used for the landscape measurement of the sensor library, and a Verification Plasmid (pVER, Figure 1b) used to verify the function of approximately 120 sensors from the library chosen to test the accuracy of the landscape measurement and to demonstrate the range of sensor function. We also describe the protocol used for the creation of the LacI sensor library in E. coli.
Preparation of a protein-LG conjugated to horseradish peroxidase by the periodate method. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

SpLG comprises of 4 Ig-binding domains of SpL and 2 IgG Fc-binding SpG domains [1]. This hybrid molecule was found to bind many intact human Ig molecules and Ig fragments [1]. It proved a powerful tool for the binding, detection and purification of antibodies [2].It was reported that chimeric SpLG was a potent mitogen for mouse splenic B cells, and induced cell differentiation and the production of immunoglobulins. Inhibition experiments demonstrated that the Ig-binding capacity of both SpG and SpL in the chimeric molecule are independent of each other. It was also shown that SpLG selectively absorbed Igs present in the sera of humans, rabbits, mice and rats [1-3]. The preparation of SpLG-HRP by the periodate method is a novel application [4]. References 1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20. 2. Kihlberg BM, Sjöbring U, Kastern W, Björck L. Protein LG: a hybrid molecule with unique immunoglobulin binding properties. J Biol Chem 1992, 267(35): 25583-8. 3. Justiz-Vaillant AA, McFarlane-Anderson N, and Smikle M. “Bacterial Immunoglobulin (Ig)-Receptors: Past and Present Perspectives.” American Journal of Microbiological Research, vol. 5, no. 2 (2017): 44-50. doi: 10.12691/ajmr-5-2-4. 4. Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi:10.4172/2155-9872.1000175.
Competitive ELISA to study the inhibition of the SpA- binding to Ab-3 by Ab-2. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

This immunoassay tested the ability of anti-anti-SpA (Ab-2) to inhibit or interfere with the binding of anti-anti-anti-SpA (Ab-3) to the original antigen- staphylococcal protein A (SpA). It is a confirmation test of the functional capacity of Ab-2. In this ELISA, both Ab-2 and the antigen SpA compete for binding to Ab-3. When Ab-2 is present, it blocks the binding of HRP-labeled SpA to Ab-3, resulting in inhibition of reaction color development.
Preparation of a protein-LA conjugated to horseradish peroxidase by the periodate method. v1
Authors: A Justiz-Vaillant Angel
Published: August 2020

Protein LA, a novel hybrid protein, structurally contains 4 of the Ig Fc-binding and 4 of the Ig Fab binding regions on SpA with 4 of kappa light chain-binding sites of protein L. It has a MW of 65 kDa. Protein LA combines the binding properties of the both SpL and SpA and in some cases, give higher binding affinity than either protein alone. The binding of an Ig to SpL does not interfere with binding of another Ig molecule to the SpA domains and vice versa. Protein LA has been shown to bind effectively to immunoglobulins and their fragments from many species of animals [1]. Reference 1. Justiz-Vaillant AA, Akpaka PE, McFarlane-Anderson N, Smikle MF. Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species.West Indian Med J. 2013;62(1):12-20.
Gestão da diversidade no Brasil: artigos publicados na base Spell no período 2006 - 2016 ## Diversity management in Brazil: articles published in the Spell base in the period 2006 - 2016
Authors: Marques Junior Eles Calheiros , Marques Ana Luzia De Barros Andrade , Dantas Anderson De Barros
Published: August 2020

ResumoO objetivo deste trabalho é analisar a produção científica brasileira em gestão da diversidade, publicada na base Spell, no período de 2006 a 2016. Trata-se de uma pesquisa descritiva voltada a indicação das seguintes características: quantitativo dos artigos publicados por ano, identificação dos periódicos e suas respectivas classificações Qualis CAPES, padrão de autoria dos artigos e identificação dos pesquisadores com mais publicações, indicação dos centros geográficos e institucionais difusores da discussão sobre a temática, e apresentação dos eixos da diversidade mais recorrentes nos artigos selecionados, considerando a classificação proposta por Pinheiro e Gois (2013). Os resultados permitem afirmar que a gestão da diversidade apresentou uma produção científica com destacada oscilação anual; que há uma concentração da produção nos estados e instituições do Sudeste e Sul do Brasil; há uma pulverização dos artigos nos periódicos da área de Administração, com média de 1,29 artigos por periódico; e que as publicações analisadas abordaram com mais frequência à diversidade em um contexto geral, seguida pela discussão das dimensões gênero e pessoas com deficiência. Um número reduzido de artigos tratou da diversidade racial e de orientação sexual, e os eixos geracional e imigracional quase não foram contemplados nos artigos sobre gestão da diversidade no Brasil. AbstractThe objective of this work is to analyze the Brazilian scientific production in diversity management, published in the Spell database, from 2006 to 2016. It is a descriptive research, aimed at indicating the following characteristics: quantitative of the published articles per annum; identification of journals and their respective Qualis CAPES; standard of authorship of the articles and identification of the most productive researchers; indication of the geographical and institutional centers that disseminate the discussion on the subject; and presentation of the most recurrent themes of diversity in selected articles, considering the classification proposed by Pinheiro and Gois (2013). The results allow to affirm that the management of diversity presented a scientific production with outstanding annual oscillation; that there is a concentration of production in the states and institutions of the Southeast and South of Brazil; there is a spraying of articles in the periodicals of Administration, with an average of 1.29 articles per period; and that the publications analyzed addressed diversity more frequently in a general context, followed by the discussion of the dimensions of gender and people with disabilities.
Divulgação de passivos ambientais no Pará: uma análise das autuações do Ibama ## Disclosure of environmental liabilities in Pará: an analysis of Ibama's authorations
Authors: Souza Vanessa Magalhães
Published: August 2020

ResumoAo desenvolver suas atividades, as entidades que possuem relação com o meio ambiente podem gerar impactos ambientais negativos e em discordância da Lei 9.605/98 de Crimes Ambientais, gerando passivos. A contabilidade é a ferramenta de divulgação das informações contábil-financeiras aos usuários externos, através das demonstrações contábeis. Em se tratando de divulgação de passivos ambientais relacionados a floresta amazônica, destaca-se o Estado do Pará que abriga a floresta amazônica, patrimônio natural da humanidade, e é líder nas autuações aplicadas pelo IBAMA. Diante deste contexto formulou-se o seguinte problema de pesquisa: Como as entidades constituídas como sociedades anônimas autuadas por crimes ambientais no Estado do Pará divulgam em suas demonstrações contábeis esses passivos ambientais? Sendo o objetivo deste estudo verificar se as entidades constituídas como sociedades anônimas autuadas por crimes ambientais incorridos no Estado do Pará divulgam as multas e os processos relacionados a essas autuações em suas demonstrações contábeis. O estudo foi conduzido a partir de pesquisa documental através de técnica de análise de conteúdo, onde foram investigados os dados das demonstrações contábeis contrapondo o relatório de autuações ambientais extraídos do sítio do IBAMA de 9 entidades autuadas no período de janeiro de 2015 a dezembro de 2018. Os resultados mostram que apenas uma empresa da amostra evidencia em balanço e 55,55% da amostra cita autuações em nota explicativa sem vincular à região afetada. AbstractIn developing their activities, entities that have a relationship with the environment may generate negative environmental impacts and in disagreement with Law 9605/98 on Environmental Crimes, generating liabilities. On the other hand, accounting is the tool for disclosing accounting-financial information to external users through the financial statements. In the case of disclosure of environmental liabilities related to the Amazon forest, the State of Pará is home to the Amazon forest, a natural patrimony of mankind, and is the leader in the assessments applied by IBAMA. In view of this context, the following research problem was formulated: Howentities constituted as corporations assessed for environmental crimes in the State of Pará disclosing these environmental liabilities in their financial statements? The purpose of this study is to verify if entities constituted as corporations assessed for environmental crimes incurred in the State of Pará disclose fines and proceedings related to these assessments in their financial statements.
A ocorrência do caramujo terrestre Megalobulimus em um sítio arqueológico de Mato Grosso do Sul, Brasil.
Authors: Aguiar Rodrigo Luiz Simas de
Published: August 2020

A ocorrência de caramujos terrestres é citada por autores da área de arqueologia. No sul e sudeste do Brasil, há descrições mais detalhadas sobre a malacofauna presente em sítios arqueológicos, tanto do litoral como do planalto. Entretanto, em Mato Grosso do Sul, estudos dessa natureza são incipientes, tornando necessário o aprofundamento das discussões a respeito. Este artigo trata da ocorrência de Megalobulimus no sítio arqueológico Templo dos Pilares, em Alcinópolis (MS). No nível 10 de uma das quadrículas recuperou-se uma concha intacta desta espécie, sendo datada de 4.141 A. P.
Peculiarities of formation of the Eubacterial complex of winter wheat rhysosphere (Triticum durum) by different fertilization systems
Authors: Hudz S. , Skivka L.
Published: August 2020

The of the study was to determine the quantitative, qualitative composition and taxonomic structure of the eubacterial complex in the rhizosphere of winter wheat using different fertilizer systems.Methods.Microbiological methods were used to determine the content of winter microorganisms in the wheat rhizosphere, and molecular taxonomic structure and methane of the eubacterial complex of microorganisms were determined. Results.It was found that among large phyla on the variants of the biological system of winter wheat fertilizer the share of Proteobacteria increased to 80,3%, and the number of Actinobacteria decreased to 12.4% according to the control variant 76,3 and 17,5 %, also similar growth rates were marked on the variant of the ecological fertilizer system. The analysis of families shows that such families as Alcaligenaceae and Pseudomonadaceae were dominant in winter wheat crops with the use of different fertilizer variants. Under the industrial fertilizer system, the share of the family Alcaligenaceae was the highest, but with the application of biological and ecological systems it decreased, while the prevalence of the family Pseudomonadaceae on the contrary – increased. The use of organic fertilizers, compared with mineral, contributed to the formation of a variety of bacteria, so the greatest diversity of prokaryotes according to the Shannon index was in the biological fertilizer system – 4,82, and the least – in the industrial version of winter wheat fertilizer. Conclusions. It was found that in the variant of the biological fertilization system the increase of species diversity was due to the following phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, Verrucomicrobia, and in the ecological fertilization system due to such philos as: Actinobacteria, Bacteroidetes, Firmicutes and Proteobac. In general, according to the experiment, the absolute dominants were representatives of bacterial philosProteobacteria – 79,1 %, Actinobacteria – 14,0%.
TESLA VERTICAL INTEGRATION STRATEGIES: THEORY, PRACTICE, RESULTS
Authors: Movsesyan E. K. , Anokhina M. E.
Published: August 2020

The purpose of this article is to disclose the content of vertical integration strategies using Tesla as an example. In the theoretical part, the typology of integrated growth strategies is considered, and the feasibility of their application is substantiated. The practical part presents the experience of using such strategies with the Tesla electric vehicle manufacturer in the turbulent environment of the company.
New Sorption Properties of Diatomaceous Earth for Water Desalination and Reducing Salt Stress of Plants
Authors: Alsar Zh. , Duskinova B. , Insepov Z.
Published: August 2020

A new practical application of the unique sorption abilities of Diatomaceous Earth (DE) or diatomite, a widely accessible and promising natural mineral, has been studied. By analyzing aqueous extracts of natural diatomite, it was shown that DE probably contains various inorganic salts, which are released into the solution in the form of ions, such as Cl-, SO42-, Na+, Ca2+, Mg2+, K+ and, apparently, others. Diatomite was able to exchange these ions with the environment, exhibiting the properties of a natural ion exchanger. Studying the kinetics of ion release from diatomite showed that the ion desorption process continues for 4‒5 h until the surrounding solution is saturated with ions, after which it is dynamically balanced by the sorption process. In order to significantly reduce the ionic content of diatomite, DE samples were processed in a technologically simple and environmentally friendly way. Thus, as a result of deionization, the content of ions released from diatomite significantly decreases. Deionized diatomite was applied to study the adsorption of sodium and chloride ions from aqueous solutions. The maximum adsorption was 50.2 mg/g, and the maximum degree of extraction, corresponding to the concentration range of 5‒100 mg/l, was 53.9%. The observed effect was also applicable for increasing the resistance of plants to salt stress, improving the germination and growth of wheat samples. The developed method can be used in the manufacturing of filters for water desalination, both drinking and technological; in ecology; in agriculture to reduce salt stress of plants, as well as for the restoration of lands contaminated by salt.
Effect of Different Pore-Forming Additives on the Formation of PVDF Microporous Membranes for Bucky-Gel Actuator
Authors: Morozov O.S. , Shachneva S.S. , Bulgakov B.A. , Babkin A.V. , Kepman A.V.
Published: August 2020

The microporous polyvinylidene fluoride (PVDF) membranes were prepared by the solvent evaporation method using 50 wt.% of different pore-forming additives: poly(1-ethyl-3-vinylimidazolium tetrafluoroborate) (PIL-BF4), polyethylene glycol 3000 (PEG-3K) and 40000 (PEG-40K), dibutyl phthalate (DBP). The influence of used additive on morphology, porosity, degree of crystallinity, tensile properties, electrolyte uptake and ionic conductivity of the membranes were investigated. The maximum electrolyte uptake of 1-ethyl-3-methylimidazolium tetrafluoroborate (EMImBF4) was 184 wt.% for the membrane prepared with PEG-40K, however, the membrane was fragile and unsuitable for practical use. The remaining membranes showed approximately the same porosity (45‒48%) and electrolyte uptakes (169‒175%). At the same time, the membranes significantly differed in mechanical properties and ionic conductivity. The membrane prepared with PIL-BF4, unlike others, has a sponge-like structure and demonstrated high mechanical properties, namely tensile strength is 17.7 MPa and fracture strain is 132.5%. Bucky gel actuators were fabricated using membranes prepared with different additives. The blocking force of the actuators based on membranes with different additives decreased in the sequence of PIL-BF4, DBP and PEG. The actuator based on the membrane prepared with PIL-BF4 demonstrates a blocking force of 5.7 mN and a deformation of 1.35 % at 3 V DC.
A study on milk quality testing and awareness of science and non science students on milk adulteration
Authors: Fatima Sadath , Vasundhara AVE
Published: August 2020

Increased global demand for milk has made it prone to massive levels of adulteration. The aims of this study were to analyze the quality of milk samples and also to assess the awareness of science and non-science students on milk adulteration. A total of 100 milk samples [unbranded (n=50) and branded (n=50)] were randomly collected from different areas of Hyderabad and were chemically analyzed to detect the presence of adulterants using standard procedures. A pre-tested questionnaire was used to assess the knowledge of students (sciences and non-sciences) on milk adulteration. It was observed from the chemical analysis that the unbranded milk samples (n=50) and branded milk samples (n=50) were found to be adulterated with water, cane sugar, urea, detergent, skimmed milk powder and formalin with their extent being 82%, 24%, 12%, 36%, 26%, 22% and 6%, 2%, 0%, 0%, 36%, 28% respectively. It was found from a microbiological examination that 92% of branded milk samples were in good quality, 2% were in fair quality and 6% were in poor quality and all the unbranded milk samples were found to be of poor quality. A significant difference (p<0.05) was observed on the awareness of milk adulteration between Science and Non-Science student respondents. It can be concluded that milk quality is not as per standards. To eradicate this Malpractice, having a quality control system is necessary which could regularly check to ensure good quality milk is sold.
Assessment of compliance of butcher shops with food safety practices in Rungwe district Tanzania
Authors: Mbonabucha Danford Barnaba , Tungaraza Fweja Leonard William
Published: August 2020

The present study examined the compliance of butcher shops to food safety practices. The study was done based on a survey of 61 respondents randomly sampled from 61 butchers in Rungwe district. Data were analyzed using IBM SPSS Statistics version 20 using descriptive statistics. The results indicated that the butcher environment and physical conditions had an average compliance score of 50.8%, the overall average awareness score on foodborne illnesses and hygienic practices was 65% whereas cleaning and sanitization had an overall average compliance score of 41.9%. Personal hygiene which was evaluated in terms of clothing, hands and individual health recorded 75.4% and 68.9% compliance scores for proper handwashing and drying respectively. The common hand drying practices involved the use of clean towels (62.8%) and disposable paper towels (11.5%), even so, 26.2% did not dry their hands at all. The use and cleanliness of aprons were adhered to by 93.7% of the respondents. Cross-contamination was deemed likely among 45.9% butchers due to non-separation and storage of spare clothes in the butchers. The common handling and storage practices was that of suspending meats on hooks at room temperatures (93.4%) with only 4.9% of butchers suspending on hooks at refrigeration temperature. This suggests none chilling of surplus meat at the end of the day sale by the majority of butchers. The compliance of butcher shops to food safety practices was generally modest which could be attributed to illiteracy in food safety and lack of proper meat handling and storage facilities.
Risk of bacterial foodborne pathogen infection among gastroenteritis cases in Qatar
Authors: Peters Kenlyn E. , Valenzuela Nilda , Morales Gomez Adriana.M. , Doiphode Sanjay , Ibrahim Emad E. , Sharma Manik , Sultan Ali , Mohammed Hussni O.
Published: August 2020

Foodborne illness has been determined to be one of the major limitations to the advancement of world health. Bacterial pathogens among the leading causes of foodborne illness are Escherichia coli (E. coli), Campylobacter, Salmonella, and Listeria. The risk of these pathogens was investigated among gastroenteritis cases in the diverse population of the state of Qatar. Fecal samples from patients admitted to Hamad Medical Corporation (HMC) with complaints of gastroenteritis were screened for the targeted pathogens using a combination of bacterial enrichments and molecular detection. Salmonella was the most common pathogen (42.9%), followed by E. coli (35.3%), and Campylobacter (21.0%). C. jejuni was the most common species of Campylobacter (67.4%). The probability of detection of E. coli decreased with age. Meanwhile, both probabilities of detection of Campylobacter and Salmonella increased with age. Listeria monocytogenes was much less common among gastroenteritis cases compared to the other pathogens.
Assessing plant-based coagulant performance to improve the safety and quality of water
Authors: Siddique Umera , Ahmed Naveed , Butt Masood Sadiq , Parveen Rashida , Rahman Ubaid , Ishaq Anum
Published: August 2020

The proposed work was carried out to replace the conventional water purification methods by natural coagulants. Samples of groundwater were obtained and analyzed for quality parameters. The coagulants of Moringa oleifera, orange peels, and date pits were prepared, and different combinations of these coagulants were used to find out the most effective one. Results regarding various physicochemical tests including pH, electrical conductivity, turbidity, total dissolved solids (TDS), calcium, magnesium, total hardness, bicarbonates, chlorides and heavy metals revealed that pH was not affected by treatments, values for EC were dropped down slightly but not reduced to an acceptable limit of World Health Organization (WHO). TDS, total hardness and chlorides reduced to the acceptable limit of WHO. Additionally, a significant reduction was recorded in lead and arsenic contents of treated water samples. Moreover, the results exhibited that the values for TPC and Total Coliform were dropped down slightly but not reduced to a satisfactory limit of WHO, while fecal Coliform reduced to the acceptable limit of WHO and E.coli was found to be zero in all samples. Conclusively, natural coagulants contain good adsorbent properties, which can confer the purification of water in a cost-effective way.
Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic
Authors: Komagbe Gwladys S. , Sessou Philippe , Dossa François , Sossa-Minou Prudencio , Taminiau Bernard , Azokpota Paulin , Korsak Nicolas , Daube Georges , Farougou Souaïbou
Published: August 2020

Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.