bluuebunny/crossref_metadata_2025 huggingface dataset:
Establishment of a Laminated Crushed Products Function and Numerical Analysis
Authors: Duan Guochen , Shi Boqiang , Shen Yanhua , Yu Guoqing
Published: March 2019

A model of the relative compression ratio for single-particle crushed products, based on the distinct element method (DEM) and numerical analysis, was established to describe precisely the crushed products of granular particles during laminated crushing. The relative compression ratio model was used to describe the functional relationship between the total compression ratio and the single-particle compression ratio, which can be described by lognormal distribution. The single-particle crushed products model was used to describe the functional relationship between the single-particle compression ratio and the distribution of single-particle crushed products. The distribution of single-particle crushed products was described by a three-parameter beta distribution. On the basis of the above model, the function of laminated crushed products of granular particles was established. According to the simulation results of EDEM under confining pressure, a functional relationship between total compression ratio, particle size, and height of the granular particles for laminated crushed products of granular particles was built. It was proved that the function of the theoretical particle size distribution coefficient of laminated crushed products of granular particles was not too different from the actual value determined by simulation. The function is universal and can be used to provide a theoretical basis and a design reference for the design of cone crushers, high-pressure roller mills, and other crushing equipment.
A 36-Pulse Diode Rectifier with an Unconventional Interphase Reactor
Authors: Lian Yuxin , Yang Shiyan , Ben Hongqi , Yang Wei
Published: March 2019

This paper proposes a simple 36-pulse diode rectifier with an unconventional interphase reactor (IPR) and single-phase full-wave rectifier (SFR). The primary winding of the unconventional IPR is double tapped and the secondary winding is connected with SFR. The operation mode of the unconventional IPR is analyzed, and the optimal tap ratio and turn ratio of the unconventional IPR are designed from both viewpoints of input line current and load voltage. With the optimal parameters of the unconventional IPR, the proposed rectifier extends the conventional 24-pulse rectifier operation to 36-pulse operation and draws near sinusoidal input line currents, the total harmonic distortion (THD) of which is about 5.035%. The kVA rating of the unconventional IPR is about 2.6% of the output power. Compared with the conventional 36-pulse rectifier with triple-tapped IPR, the proposed 36-pulse rectifier is easy and simple to implement instead of using control circuits. Experiment results are provided to verify the theoretical analysis.
Mapping Forest Fire Risk at a Local Scale—A Case Study in Andalusia (Spain)
Authors: Vallejo-Villalta Ismael , Rodríguez-Navas Estefanía , Márquez-Pérez Joaquín
Published: March 2019

Forest fires are a critical environmental problem facing current societies, with serious repercussions at ecological, economic and personal safety levels. Detailed maps enabling identification of areas liable to be affected is an indispensable first step allowing different prevention and protection measures vis-à-vis this kind of phenomenon. These maps could be especially valuable for use in land management and emergency planning at a municipality scale. A methodology is shown for producing local maps of mid- and short-term forest fire risk, integrating both natural and human factors. Among natural factors, variables normally used in hazard models are considered as fuel models, slopes or vegetation moisture stress. From the human perspective, more novel aspects have been evaluated, meant either to assess human-induced hazard (closeness to forestland of causative elements or the ability of people to penetrate the forest environment), or to assess vulnerability, considering the population’s location in urban centres and scattered settlements. The methodology is applied in a municipality of Andalusia (Spain) and obtained results were compared to burned areas maps.
Leaf-Associated Shifts in Bacterial and Fungal Communities in Response to Chicken Rearing Under Moso Bamboo Forests in Subtropical China
Authors: Zhang Xiaoping , Zhong Zheke , Gai Xu , Ying Jiafu , Li Weifen , Du Xuhua , Bian Fangyuan , Yang Chuanbao
Published: March 2019

Integrated bamboo-chicken farming (BCF) systems are a traditional agroforestry pattern with large economic benefits in subtropical China. However, little is known regarding the effect of this integration on the bamboo leaf-associated microbiome, which can be very important for disease control and nutrient turnover. In the present study, we compared the leaf-associated bacterial and fungal communities of moso bamboo (Phyllostachys edulis) in a BCF system and an adjacent moso bamboo forest (MBF). The results showed that Cyanobacteria and Ascomycota were the predominant microbial phyla associated with bamboo leaves. Chicken farming under the bamboo forest significantly increased the bacterial and fungal alpha diversity (observed operational taxonomic units (OTUs) and Simpson’s index) associated with bamboo leaves. Principal components analysis (PCoA) further confirmed the shifts in the bacterial and fungal communities caused by chicken farming. Based on the observed relative abundances, the phyla Bacteroidetes, Actinobacteria, TM7, and Basidiomycota were significantly increased on BCF-associated leaves compared with MBF leaves, while Acidobacteria and Ascomycota were significantly decreased. An ecological function prediction analysis based on metabolic processes indicated that BCF could accelerate nutrient (C, N, and S) cycling but may increase the risk of fungal-associated diseases. Our findings suggest that shifts in leaf-associated bacterial and fungal communities can be important indicators for the scientific management of BCF systems.
Forest Site and Type Variability in ALS-Based Forest Resource Inventory Attribute Predictions over Three Ontario Forest Sites
Authors: van Ewijk Karin , Treitz Paul , Woods Murray , Jones Trevor , Caspersen John
Published: March 2019

Over the last decade, spatially-explicit modeling of landscape-scale forest attributes for forest inventories has greatly benefitted from airborne laser scanning (ALS) and the area-based approach (ABA) to derive wall-to-wall maps of these forest attributes. Which ALS-derived metrics to include when modeling forest inventory attributes, and how prediction accuracies vary over forest types depends largely on the structural complexity of the forest(s) being studied. Hence, the purpose of this study was to (i) examine the usefulness of adding texture and intensity metrics to height-based ALS metrics for the prediction of several forest resource inventory (FRI) attributes in one boreal and two Great Lakes, St. Lawrence (GLSL) forest region sites in Ontario and (ii) quantify and compare the site and forest type variability within the context of the FRI prediction accuracies. Basal area (BA), quadratic mean diameter-at-breast height (QMD), and stem density (S) were predicted using the ABA and a nonparametric Random Forests (RF) regression model. At the site level, prediction accuracies (i.e., expressed as RMSE (Root Mean Square Error), bias, and R2) improved at the three sites when texture and intensity metrics were included in the predictor set, even though no significant differences (p > 0.05) could be detected using the nonparametric RMANOVA test. Stem density benefitted the most from the inclusion of texture and intensity, particularly in the GLSL sites (% RMSE improved up to 6%). Combining site and forest type results indicated that improvements in site level predictions, due to the addition of texture and intensity metrics to the ALS predictor set, were the result of changes in prediction accuracy in some but not all forest types present at a site and that these changes in prediction accuracy were site and FRI attribute specific. The nonparametric Kruskal–Wallis test indicated that prediction errors between the different forest types were significantly different (p ≤ 0.01). In the boreal site, prediction accuracies for conifer forest types were higher than for deciduous and mixedwoods. Such patterns in prediction accuracy among forest types and FRI attributes could not be observed in the GLSL sites. In the Petawawa Research Forest (PRF), we did detect the impact of silvicultural treatments especially on QMD and S predictions.
IoH: A Platform for the Intelligence of Home with a Context Awareness and Ambient Intelligence Approach
Authors: Gomes Luis , Ramos Carlos , Jozi Aria , Serra Bruno , Paiva Lucas , Vale Zita
Published: March 2019

This paper presents IoH (Intelligence of Home), a platform developed to test some basic intelligent behaviors in Home context. Internet of Things, ambient intelligence and context awareness approaches motivated the development of IoH. The platform involves six layers, responsible by connectivity, persistency, unification, Internet of Things integration, subsystems integration and user interface. The integrated subsystems involve intelligent systems for light control, television brightness control, desk light control, persons counting and air conditioner control. The IoH platform is then tested for a real building, and results and conclusions are obtained. Different intelligent methods and technologies are used, form the use of a diversity of sensors, actuators, and controllers and processing units to a set of artificial intelligence approaches varying from machine learning and optimization algorithms to the use of sensor fusion and computer vision. The use of IoH day-by-day demonstrated an intelligent performance for the real building occupants.
Hot Topic Community Discovery on Cross Social Networks
Authors: Wang Xuan , Zhang Bofeng , Chang Furong
Published: March 2019

The rapid development of online social networks has allowed users to obtain information, communicate with each other and express different opinions. Generally, in the same social network, users tend to be influenced by each other and have similar views. However, on another social network, users may have opposite views on the same event. Therefore, research undertaken on a single social network is unable to meet the needs of research on hot topic community discovery. “Cross social network” refers to multiple social networks. The integration of information from multiple social network platforms forms a new unified dataset. In the dataset, information from different platforms for the same event may contain similar or unique topics. This paper proposes a hot topic discovery method on cross social networks. Firstly, text data from different social networks are fused to build a unified model. Then, we obtain latent topic distributions from the unified model using the Labeled Biterm Latent Dirichlet Allocation (LB-LDA) model. Based on the distributions, similar topics are clustered to form several topic communities. Finally, we choose hot topic communities based on their scores. Experiment result on data from three social networks prove that our model is effective and has certain application value.
From Heraldry to Genealogy from Silverware
Authors: Durie Bruce
Published: March 2019

A Coat of Arms engraved on a piece of silverware allowed the identification of the parties concerned, and the elucidation of the details of their marriage and ancestries. The Arms themselves have an interesting provenance.
RNA Viruses as Tools in Gene Therapy and Vaccine Development
Authors: Lundstrom Kenneth
Published: March 2019

RNA viruses have been subjected to substantial engineering efforts to support gene therapy applications and vaccine development. Typically, retroviruses, lentiviruses, alphaviruses, flaviviruses rhabdoviruses, measles viruses, Newcastle disease viruses, and picornaviruses have been employed as expression vectors for treatment of various diseases including different types of cancers, hemophilia, and infectious diseases. Moreover, vaccination with viral vectors has evaluated immunogenicity against infectious agents and protection against challenges with pathogenic organisms. Several preclinical studies in animal models have confirmed both immune responses and protection against lethal challenges. Similarly, administration of RNA viral vectors in animals implanted with tumor xenografts resulted in tumor regression and prolonged survival, and in some cases complete tumor clearance. Based on preclinical results, clinical trials have been conducted to establish the safety of RNA virus delivery. Moreover, stem cell-based lentiviral therapy provided life-long production of factor VIII potentially generating a cure for hemophilia A. Several clinical trials on cancer patients have generated anti-tumor activity, prolonged survival, and even progression-free survival.
Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in Arabidopsis thaliana
Authors: Shahid Muhammad , Imran Qari , Hussain Adil , Khan Murtaza , Lee Sang , Mun Bong , Yun Byung-Wook
Published: March 2019

Plant stem cells are pluripotent cells that have diverse applications in regenerative biology and medicine. However, their roles in plant growth and disease resistance are often overlooked. Using high-throughput RNA-seq data, we identified approximately 20 stem cell-related differentially expressed genes (DEGs) that were responsive to the nitric oxide (NO) donor S-nitrosocysteine (CySNO) after six hours of infiltration. Among these DEGs, the highest number of positive correlations (R ≥ 0.8) was observed for CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) 12. Gene ontology (GO) terms for molecular function showed DEGs associated with signal transduction and receptor activity. A promoter study of these DEGs showed the presence of cis-acting elements that are involved in growth as well as the regulation of abiotic and biotic stress. Phylogenetic analysis of the Arabidopsis stem cell-related genes and their common orthologs in rice, soybean, poplar, and tomato suggested that most soybean stem cell-related genes were grouped with the Arabidopsis CLE type of stem cell genes, while the rice stem cell-related genes were grouped with the Arabidopsis receptor-like proteins. The functional genomic-based characterization of the role of stem cell DEGs showed that under control conditions, the clv1 mutant showed a similar phenotype to that of the wild-type (WT) plants; however, under CySNO-mediated nitrosative stress, clv1 showed increased shoot and root length compared to WT. Furthermore, the inoculation of clv1 with virulent Pst DC3000 showed a resistant phenotype with fewer pathogens growing at early time points. The qRT-PCR validation and correlation with the RNA-seq data showed a Pearson correlation coefficient of >0.8, indicating the significantly high reliability of the RNA-seq analysis.
Pharmacogenomic and Pharmacotranscriptomic Profiling of Childhood Acute Lymphoblastic Leukemia: Paving the Way to Personalized Treatment
Authors: Pavlovic Sonja , Kotur Nikola , Stankovic Biljana , Zukic Branka , Gasic Vladimir , Dokmanovic Lidija
Published: March 2019

Personalized medicine is focused on research disciplines which contribute to the individualization of therapy, like pharmacogenomics and pharmacotranscriptomics. Acute lymphoblastic leukemia (ALL) is the most common malignancy of childhood. It is one of the pediatric malignancies with the highest cure rate, but still a lethal outcome due to therapy accounts for 1–3% of deaths. Further improvement of treatment protocols is needed through the implementation of pharmacogenomics and pharmacotranscriptomics. Emerging high-throughput technologies, including microarrays and next-generation sequencing, have provided an enormous amount of molecular data with the potential to be implemented in childhood ALL treatment protocols. In the current review, we summarized the contribution of these novel technologies to the pharmacogenomics and pharmacotranscriptomics of childhood ALL. We have presented data on molecular markers responsible for the efficacy, side effects, and toxicity of the drugs commonly used for childhood ALL treatment, i.e., glucocorticoids, vincristine, asparaginase, anthracyclines, thiopurines, and methotrexate. Big data was generated using high-throughput technologies, but their implementation in clinical practice is poor. Research efforts should be focused on data analysis and designing prediction models using machine learning algorithms. Bioinformatics tools and the implementation of artificial i Lack of association of the CEP72 rs924607 TT genotype with intelligence are expected to open the door wide for personalized medicine in the clinical practice of childhood ALL.
Using Three Cross-Sectional Surveys to Compare Workplace Psychosocial Stressors and Associated Mental Health Status in Six Migrant Groups Working in Australia Compared with Australian-Born Workers
Authors: Daly Alison , Carey Renee N. , Darcey Ellie , Chih HuiJun , LaMontagne Anthony D. , Milner Allison , Reid Alison
Published: March 2019

Migrant workers may be more likely to be exposed to workplace psychosocial stressors (WPS) which have an affect on physical and mental health. Given the relative lack of research on this topic, the study objectives were to estimate and compare the prevalence of WPS in migrant and Australian workers and investigate associated mental health problems. Three cross-sectional surveys, two with migrant workers and one with Australian workers, were pooled to provide estimates of prevalence. Regressions were conducted to investigate associations between workers and WPS. All WPS, except unfair pay, were associated with higher probability of mental health problems. The association between WPS and mental health did differ between some migrant groups. Compared with Australian-born workers, all other migrant groups tended to have a lower risk of mental health outcomes. Interactions between WPS and migrants showed variable levels in the risk of having a mental health problem, some attenuated and some increased. The study showed that country of birth does play a part in how treatment in the workplace is perceived and responded to. Any interventions to improve workplace conditions for migrant workers need to be aware of the different experiences related to migrant ethnicity.
Florida Arsenic Distribution Index: Quantifying the Distribution of Past and Present Arsenic Usage
Authors: Scott-Richardson Maya , O’Hara Ruiz Marilyn , Smith Rebecca L.
Published: March 2019

Arsenic is an abundant, highly toxic element that is a global health concern due to damage from acute and chronic exposure and the potential for high local concentrations in heavily populated areas. In Florida, arsenic has been used heavily in agricultural, commercial, and industrial applications for decades. While studies have identified and quantified the contributions of arsenic to the state, there are fewer studies that have attempted to index to identify spatial distribution patterns. The aim of this study was to develop representative indices that would identify and estimate the distribution of arsenic from historic and present usage for the state of Florida at the county-level. Eight variables are summarized and categorized into two different types of arsenic indices that represent the arsenic distribution from natural occurrence and anthropogenic practices in Florida. The anthropogenic index had distributions scores that ranged from 0.20 to 1.60 with a mean of 0.61 (SD = 0.34). The natural index had distribution scores that ranged from 1.00 to 3.00 and a mean of 1.47 (SD = 0.43). Our finding noticed comparability between high arsenic distributions mainly occur in counties located in the northwestern and southwestern regions in both the anthropogenic and natural indices with diverse arsenic sources contributions.
Using Photovoice to Examine Physical Activity in the Urban Context and Generate Policy Recommendations: The Heart Healthy Hoods Study
Authors: Gullón Pedro , Díez Julia , Conde Paloma , Ramos Carmen , Márquez Valentín , Badland Hannah , Escobar Francisco , Franco Manuel
Published: March 2019

A current challenge in physical activity research is engaging citizens with co-creating policies that support physical activity participation. Using Photovoice, a participatory action research method, the objectives of this study were to: 1) Identify community perceptions of urban built, social, and political/economic environment factors associated with physical activity; and 2) generate community-driven policy recommendations to increase physical activity. Two districts in Madrid of varying socio-economic status (SES) were selected. Overall, 24 residents participated in 4 groups stratified by sex and district (6 participants per group). Groups met weekly for 4 weeks to discuss and analyze their photographs. Participants coded photographs into categories, which were then regrouped into broader themes. The categories were transformed into policy recommendations using an adaptation of the logical framework approach. Participants took 161 photos, which were classified into 61 categories and 14 broader themes (e.g., active transportation, sport in the city). After this, participants generated a set of 34 policy recommendations to improve the urban environment to support physical activity (e.g., to redistribute sports facilities). Collaboration between citizens and researchers led to a deeper understanding of the community perceptions of urban built, social, and political/economic environment factors associated with physical activity in two districts of Madrid, while engaging citizens in recommending public policies.
The Effects of Tai Chi on Markers of Atherosclerosis, Lower-limb Physical Function, and Cognitive Ability in Adults Aged Over 60: A Randomized Controlled Trial
Authors: Zhou Shengwen , Zhang Yanjie , Kong Zhaowei , Loprinzi Paul D. , Hu Yang , Ye Jiajie , Liu Shijie , Yu Jane Jie , Zou Liye
Published: March 2019

Objective: The purpose of this study was to investigate the effects of Tai Chi (TC) on arterial stiffness, physical function of lower-limb, and cognitive ability in adults aged over 60. Methods: This study was a prospective and randomized 12-week intervention trial with three repeated measurements (baseline, 6, and 12 weeks). Sixty healthy adults who met the inclusion criteria were randomly allocated into three training conditions (TC-24, TC-42, and TC-56) matched by gender, with 20 participants (10 males, 10 females) in each of the three groups. We measured the following health outcomes, including markers of atherosclerosis, physical function (leg power, and static and dynamic balance) of lower-limb, and cognitive ability. Results: When all three TC groups (p < 0.05) have showed significant improvements on these outcomes but overall cognitive ability at 6 or 12 weeks training period, TC-56 appears to have superior effects on arterial stiffness and static/dynamic balance in the present study. Conclusions: Study results of the present study add to growing body of evidence regarding therapeutic TC for health promotion and disease prevention in aging population. Future studies should further determine whether TC-42 and TC-56 are beneficial for other non-Chinese populations, with rigorous research design and follow-up assessment.
Establishment and Application of an Evaluation Model for Orchid Island Sustainable Tourism Development
Authors: Chen Han-Shen
Published: March 2019

Global warming and climate change increase the likelihood of weather-related natural disasters that threaten ecosystems and consequently affect the tourism industry which thrives on the natural attributes of island regions. Orchid Island, the study area, is home to the Yami (Tao) tribe—the only indigenous people of Taiwan with a marine culture. The island possesses rich geological and topographical features (such as coral reefs) and distinctive biological and ecological resources (such as the green sea turtle, flying fish, and Orchid Island scops owl), and organizes traditional festivals and activities (such as the flying fish festival) as well as tribal tourism activities. These factors contribute to its immense potential to become the new tourism hotspot. To study the factors enhancing tourist experiences, a random utility model was constructed using a choice experiment method (CEM) for the tourist resort on Orchid Island. The study results demonstrated that: (1) Limiting tourists to 600/day; employing professional tour guides; providing better recreational facilities; introducing additional experience-enhancing activities; and lowering contributions towards the professional ecosystem conservation trust fund will improve the overall effectiveness of attracting tourists to Orchid Island. The evaluation results from both conditional logit and random parameter logit models were similar; (2) the analysis results from the latent class model demonstrated that island tourism has significant market segmentation. The socioeconomic backgrounds of tourists, their experiences, and their preferences exhibit heterogeneity, with significant differences in willingness to pay for island tourism.
The Effects of Indoor High Temperature on Circadian Rhythms of Human Work Efficiency
Authors: Zheng Guozhong , Li Ke , Bu Wentao , Wang Yajing
Published: March 2019

Indoor non-air-conditioned environments widely exist in the summer high temperature weather. The work efficiency of the people who stay indoors for a long time is seriously affected by the indoor high temperature. In this paper, the changes of the circadian rhythms of work efficiency in indoor high temperature environments were studied. Ten healthy subjects (five males and five females) were selected in the experiments randomly. In each experiment day, the maximum hourly outdoor temperature was selected as 28 °C, 32 °C, 36 °C, and 38 °C, respectively, to determine the experiment conditions. In each experiment condition, subjects’ response time, accuracy rate, grip strength, work willingness, and physiological parameters were monitored for 24 consecutive hours. Meanwhile, the hourly outdoor temperatures of the experiment day were accessed from the weather report during the experiment. Then the cosinor method and statistical method were adopted. The results indicated that the response time, grip strength, and work willingness followed circadian rhythms. However, the accuracy rates of the Stroop color-word test (SCWT) and numeral inspection task (NIT) did not show an obvious circadian rhythm. The effects of high temperature on the circadian rhythms of grip strength and work willingness were mainly reflected in the decreases of the median and amplitude. The effects on the response time were mainly reflected in the decrease of the median. In addition, forehead temperature showed a significant negative correlation to response time, and it could be considered as a predictor to assess the level of work efficiency. This study gives an alternative method to replace direct measurement of the ability indices at work site and provides basic data of 24 consecutive hours, for showing changes in human work efficiency. It could be helpful to predict the low performance in advance to reduce occupational accidents.
Mining Social Media to Identify Heat Waves
Authors: Cecinati Francesca , Matthews Tom , Natarajan Sukumar , McCullen Nick , Coley David
Published: March 2019

Heat waves are one of the deadliest of natural hazards and their frequency and intensity will likely increase as the climate continues to warm. A challenge in studying these phenomena is the lack of a universally accepted quantitative definition that captures both temperature anomalies and associated mortality. We test the hypothesis that social media mining can be used to identify heat wave mortality. Applying the approach to India, we find that the number of heat-related tweets correlates with heat-related mortality much better than traditional climate-based indicators, especially at larger scales, which identify many heat wave days that do not lead to excess mortality. We conclude that social media based heat wave identification can complement climatic data and can be used to: (1) study heat wave impacts at large scales or in developing countries, where mortality data are difficult to obtain and uncertain, and (2) to track dangerous heat wave events in real time.
Bisphenol S Adsorption Behavior on Ferralsol and Biochar Modified Soil with Dissolved Organic Matter
Authors: Zhang Shiqiu , Yang Xue , Liu Le , Zheng Kui , Ju Meiting , Liu Jinpeng
Published: March 2019

Bisphenol S (BPs) has been found in a variety of common consumer products surrounding human living, despite the fact that it could damage the human digestive system and genital system. In China, straw-returning to the field is a common soil improvement technology used to increase the concentration of dissolved organic matter (DOM), which plays an important role in the natural environment as a microreactor of contaminants. Additionally, the biochar obtained by the straw is an effective soil conditioner. DOM is a key influencing factor when biochar is employed as the conditioner of BPs contaminated soil. However, the BPs adsorption behavior on the Ferralsol affected by DOM and biochar is also unclear. Hence, DOM was prepared and the effect of DOM on the BPs adsorption behavior on soil and biochar modified soil was investigated. DOM was characterized by Elemental analysis, Fourier transforming infrared spectra (FT-IR), and three-dimensional excitation-emission matrix spectra (3D-EEM). The results of the adsorption experiments indicated that both biochar and DOM could improve the BPs adsorption capacity in Ferralsol, while DOM suppressed the BPs adsorption capacity of biochar modified soil, indicating that DOM and BPs could not be applied at the same time for BPs adsorption.
A Hazard Assessment Method for Waterworks Systems Operating in Self-Government Units
Authors: Rak Janusz R. , Tchórzewska-Cieślak Barbara , Pietrucha-Urbanik Katarzyna
Published: March 2019

Informing users of waterworks systems about the quality of tap water is an obligatory trend. It should be accompanied by studies on the influence of the risk of threats on public health. Waterworks systems, being included in a critical infrastructure of the city, should be subject to special protection in this respect. In the paper, the authors’ method of assessing threats to people and property from waterworks systems functioning in self-government units (SGUs), is proposed. Four categories of factors affecting the risk of threat to tap water consumers were assumed: the frequency or the probability of exposure—P, financial losses—C, damages to peoples’ health—HL, the degree of the security—S. Based on this, a four-parametric risk matrix was developed. It was assumed that risk is a function of the parameters mentioned above: r = f(P, C, HL, S). For every parameter the five-parametric weight scale was assumed. An example of applying the method is presented. The proposed method should be an important element of water safety plans. It can also be adopted for other municipal systems subject to SGU.
Barriers and Enabling Factors Affecting Satisfaction and Safety Perception with Use of Bicycle Roads in Seoul, South Korea
Authors: Sharma Bimala , Nam Hae Kweun , Yan Wanglin , Kim Ha Yun
Published: March 2019

Cycling has proven to be an important strategy in decreasing the risk of non-communicable diseases. This study aimed to discover barriers and enabling factors influencing satisfaction and safety perceptions towards the use of bicycle roads in the Seoul metropolitan area, South Korea. A cross-sectional survey of 190 youth and adult individuals was conducted. Sex, age, purpose of bicycle use, perceived safety, availability of facilities, road gradient, road width, and traffic on the bicycle road were associated with cycling regularity. Multivariate regression analysis showed that the sufficiency of bicycle parking space, moderate slope, and enough bicycle signs were significant enabling factors for satisfaction with the use of bicycle roads. Narrow bicycle roads were found to be a barrier to satisfaction with the use of bicycle roads. Moderate slope, enough bicycle signs, and enough maintenance facilities around bike roads were found to be enabling factors in the perceived safety of the use of bicycle roads, whereas traffic on the side of the bicycle road was found to be a barrier to perceived safety. Based on these findings, we conclude that healthy cities should promote cycling behavior encouraging enabling factors and initiating attempts to improve the factors that act as barriers through urban planning.
The Concept and Technologies of Quality of Geographic Information Service: Improving User Experience of GIServices in a Distributed Computing Environment
Authors: Hu Kai , Gui Zhipeng , Cheng Xiaoqiang , Wu Huayi , McClure Stephen C.
Published: March 2019

With the wide use of web technologies, service-oriented architecture (SOA), and cloud computing, more and more geographical information systems are served as GIServices. Under such circumstance, quality of geographic information services (QoGIS) has emerged as an important research topic of geoinformatics. However, it is not easy to understand the field since QoGIS has no formal standards, which is not only in regard to server-side performance and capabilities, but is also related with the quality of experience (QoE) during user interaction with GIServices. In this paper, we compare quality of service (QoS) and QoGIS research to understand the uniqueness of QoGIS. A conceptual framework is proposed to organize and interpret QoGIS research from the perspective of quality modeling, acquisition, and application, and we discuss the status, limitations, and future directions of this area. Overall, our analysis shows that new quality metrics will evolve from existing metrics to match the needs in concrete QoGIS applications, and user preferences need to be considered in quality modeling for GIServices. We discuss three approaches for the provision of QoGIS information and find that user feedback mining is an important supplementary source of quality information. Gaps between QoS and QoGIS research suggest that the GIService performance enhancement must not only consider the unique features of spatial data models and algorithms, but also system architecture, deployment, and user spatiotemporal access behaviors. Advanced service selection algorithms must be introduced to tackle the quality optimization problems of geoprocessing workflow planning. Moreover, a QoGIS-aware GIServices framework must be established to facilitate and ensure GISerivce discovery and interaction. We believe this bibliographic review provides a helpful guide for GIS researchers.
Evaluating Young People’s Area Estimation of Countries and Continents
Authors: Lapon Lieselot , De Maeyer Philippe , Vanhaeren Nina , Battersby Sarah , Ooms Kristien
Published: March 2019

For decades, cartographers and cognitive scientists have speculated about the influence of map projections on mental representations of the world. The development of Web 2.0 and web mapping services at the beginning of the 21st century—such as Google Maps, OpenStreetMap, and Baidu Map—led to an enormous spread of cartographic data, which is available to every Internet user. Nevertheless, the cartographic properties of these map services, and, in particular, the selected map projection or the Web Mercator projection, are questionable. The goal of this study is to investigate if the global-scale mental map of young people has been influenced by the increasing availability of web maps and the Web Mercator projection. An application was developed that allowed participants of Belgium and the US to scale the land area of certain countries and continents compared to Europe or the conterminous United States. The results show that the participants’ estimation of the actual land area is quite accurate. Moreover, an indication of the existence of a Mercator effect could not be discovered. To conclude, the young people’s mental map of the world does not appear to be influenced by a specific map projection but by personal characteristics. These elements are varied and require further analysis.
Cardiovascular Effects of PCB 126 (3,3’,4,4’,5-Pentachlorobiphenyl) in Zebrafish Embryos and Impact of Co-Exposure to Redox Modulating Chemicals
Authors: Teixidó Elisabet , Barenys Marta , Piqué Ester , Llobet Joan M. , Gómez-Catalán Jesús
Published: March 2019

The developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling pathways. Therefore, we exposed zebrafish embryos to PCB126 or to several redox-modulating chemicals to study their ability to modulate the dysmorphogenesis produced by PCB126. PCB126 produced a concentration-dependent induction of pericardial edema and circulatory failure, and a concentration-dependent reduction of cardiac output and body length at 80 hours post fertilization (hpf). Among several modulators tested, the effects of PCB126 could be both positively and negatively modulated by different compounds; co-treatment with α-tocopherol (vitamin E liposoluble) prevented the adverse effects of PCB126 in pericardial edema, whereas co-treatment with sodium nitroprusside (a vasodilator compound) significantly worsened PCB126 effects. Gene expression analysis showed an up-regulation of cyp1a, hsp70, and gstp1, indicative of PCB126 interaction with the aryl hydrocarbon receptor (AhR), while the transcription of antioxidant genes (sod1, sod2; cat and gpx1a) was not affected. Further studies are necessary to understand the role of oxidative stress in the developmental toxicity of low concentrations of PCB126 (25 nM). Our results give insights into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of environmental pollutants.
Halophytic Grasses, a New Source of Nutraceuticals? A Review on Their Secondary Metabolites and Biological Activities
Authors: Faustino Maria V. , Faustino Maria A. F. , Pinto Diana C. G. A.
Published: March 2019

The Poaceae family, known as grasses, is distributed worldwide and is considered the most important group of monocotyledonous crops. Salt stress is multifactorial, therefore to survive, halophytes evolved a variety of adaptations, which include the biosynthesis of different primary and secondary metabolites. This trait enhances the accumulation of important families of compounds crucial to the prevention of a variety of chronic diseases. Besides, if proven edible, these species could cope with the increased soil salinity responsible for the decline of arable land due to their high nutritional/nutraceutical value. Herein, the phytochemical investigations performed in halophytes from the Poaceae family as well as their biological properties were explored. Among the 65 genera and 148 species of known halophytic grasses, only 14% of the taxa were studied phytochemically and 10% were subjected to biological evaluation. Notably, in the studied species, a variety of compound families, as well as bioactivities, were demonstrated, highlighting the potential of halophytic grasses.
Vascular Effects of Photodynamic Therapy with Curcumin in a Chorioallantoic Membrane Model
Authors: Buzzá Hilde Harb , Fialho de Freitas Lucas Cruz , Moriyama Lilian Tan , Teixeira Rosa Ramon Gabriel , Bagnato Vanderlei Salvador , Kurachi Cristina
Published: March 2019

Photodynamic Therapy (PDT) is a treatment that requires light, a photosensitizing agent, and molecular oxygen. The photosensitizer is activated by light and it interacts with the oxygen that is present in the cellular microenvironment. The molecular oxygen is transformed into singlet oxygen, which is highly reactive and responsible for the cell death. Therefore, PS is an important element for the therapy happens, including its concentration. Curcumin is a natural photosensitizer and it has demonstrated its anti-inflammatory and anti-oxidant effects that inhibit several signal transduction pathways. PDT vascular effects of curcumin at concentrations varying from 0.1 to 10 mM/cm2 and topical administration were investigated in a chick Chorioallantoic Membrane (CAM) model. The irradiation was performed at 450 nm, irradiance of 50 mW/cm2 during 10 min, delivering a total fluence of 30 J/cm2. The vascular effect was followed after the application of curcumin, with images being obtained each 30 min in the first 3 h, 12 h, and 24 h. Those images were qualitatively and quantitatively analyzed with a MatLAB®. Curcumin was expected to exhibit a vascular effect due to its angio-inhibitory effect. Using curcumin as photosensitizer, PDT induced a higher and faster vascular effect when compared to the use of this compound alone.
Gymnotic Delivery of LNA Mixmers Targeting Viral SREs Induces HIV-1 mRNA Degradation
Authors: Hillebrand Frank , Ostermann Philipp Niklas , Müller Lisa , Degrandi Daniel , Erkelenz Steffen , Widera Marek , Pfeffer Klaus , Schaal Heiner
Published: March 2019

Transcription of the HIV-1 provirus generates a viral pre-mRNA, which is alternatively spliced into more than 50 HIV-1 mRNAs encoding all viral proteins. Regulation of viral alternative splice site usage includes the presence of splicing regulatory elements (SREs) which can dramatically impact RNA expression and HIV-1 replication when mutated. Recently, we were able to show that two viral SREs, GI3-2 and ESEtat, are important players in the generation of viral vif, vpr and tat mRNAs. Furthermore, we demonstrated that masking these SREs by transfected locked nucleic acid (LNA) mixmers affect the viral splicing pattern and viral particle production. With regard to the development of future therapeutic LNA mixmer-based antiretroviral approaches, we delivered the GI3-2 and the ESEtat LNA mixmers “nakedly”, without the use of transfection reagents (gymnosis) into HIV-1 infected cells. Surprisingly, we observed that gymnotically-delivered LNA mixmers accumulated in the cytoplasm, and seemed to co-localize with GW bodies and induced degradation of mRNAs containing their LNA target sequence. The GI3-2 and the ESEtat LNA-mediated RNA degradation resulted in abrogation of viral replication in HIV-1 infected Jurkat and PM1 cells as well as in PBMCs.
Processivity vs. Beating: Comparing Cytoplasmic and Axonemal Dynein Microtubule Binding Domain Association with Microtubule
Authors: Tajielyato Nayere , Alexov Emil
Published: March 2019

This study compares the role of electrostatics in the binding process between microtubules and two dynein microtubule-binding domains (MTBDs): cytoplasmic and axonemal. These two dyneins are distinctively different in terms of their functionalities: cytoplasmic dynein is processive, while axonemal dynein is involved in beating. In both cases, the binding requires frequent association/disassociation between the microtubule and MTBD, and involves highly negatively charged microtubules, including non-structured C-terminal domains (E-hooks), and an MTBD interface that is positively charged. This indicates that electrostatics play an important role in the association process. Here, we show that the cytoplasmic MTBD binds electrostatically tighter to microtubules than to the axonemal MTBD, but the axonemal MTBD experiences interactions with microtubule E-hooks at longer distances compared with the cytoplasmic MTBD. This allows the axonemal MTBD to be weakly bound to the microtubule, while at the same time acting onto the microtubule via the flexible E-hooks, even at MTBD–microtubule distances of 45 Å. In part, this is due to the charge distribution of MTBDs: in the cytoplasmic MTBD, the positive charges are concentrated at the binding interface with the microtubule, while in the axonemal MTBD, they are more distributed over the entire structure, allowing E-hooks to interact at longer distances. The dissimilarities of electrostatics in the cases of axonemal and cytoplasmic MTBDs were found not to result in a difference in conformational dynamics on MTBDs, while causing differences in the conformational states of E-hooks. The E-hooks’ conformations in the presence of the axonemal MTBD were less restricted than in the presence of the cytoplasmic MTBD. In parallel with the differences, the common effect was found that the structural fluctuations of MTBDs decrease as either the number of contacts with E-hooks increases or the distance to the microtubule decreases.
Thymoquinone Selectively Kills Hypoxic Renal Cancer Cells by Suppressing HIF-1α-Mediated Glycolysis
Authors: Lee Yoon-Mi , Kim Geon-Hee , Park Eun-Ji , Oh Taek-In , Lee Sujin , Kan Sang-Yeon , Kang Hyeji , Kim Byeong Mo , Kim Ji Hyung , Lim Ji-Hong
Published: March 2019

Several reports have shown that thymoquinone (TQ) effectively attenuates angiogenesis in cancer cells, resulting in suppression of tumor growth. However, it is not yet clear whether TQ reduces hypoxia-inducible factor-1α (HIF-1α) expression in hypoxic cancer cells. Here, we found that TQ was a novel HIF-1α inhibitor through hypoxia response element (HRE)-luciferase assay-based large screening by using 502 natural compounds containing chemical library. TQ reduced HIF-1α protein levels in renal cancer cells; however, it did not affect the HIF-1α protein levels in the presence of proteasome inhibitor, MG132, indicating that the reduction effects of TQ on HIF-1α protein are mediated via the ubiquitination-proteasome dependent pathway. TQ boosted HIF-1α protein degradation, and the mechanism was revealed by inhibiting interaction between HSP90 and HIF-1α. TQ suppressed downstream genes of HIF-1α, indicating negative impact of TQ on HIF-1α transcriptional activities. In addition, TQ altered glucose, lactate, and ATP levels, leading to anaerobic metabolic disturbance. TQ induced apoptosis in hypoxic cancer cells as determined by crystal violet staining and flow cytometry for annexin V-stained cells. Taken together, we suggested that TQ is a potential anticancer agent targeting HIF-1α.
Refining the Phenotype of Recurrent Rearrangements of Chromosome 16
Authors: Redaelli Serena , Maitz Silvia , Crosti Francesca , Sala Elena , Villa Nicoletta , Spaccini Luigina , Selicorni Angelo , Rigoldi Miriam , Conconi Donatella , Dalprà Leda , Roversi Gaia , Bentivegna Angela
Published: March 2019

Chromosome 16 is one of the most gene-rich chromosomes of our genome, and 10% of its sequence consists of segmental duplications, which give instability and predisposition to rearrangement by the recurrent mechanism of non-allelic homologous recombination. Microarray technologies have allowed for the analysis of copy number variations (CNVs) that can contribute to the risk of developing complex diseases. By array comparative genomic hybridization (CGH) screening of 1476 patients, we detected 27 cases with CNVs on chromosome 16. We identified four smallest regions of overlapping (SROs): one at 16p13.11 was found in seven patients; one at 16p12.2 was found in four patients; two close SROs at 16p11.2 were found in twelve patients; finally, six patients were found with atypical rearrangements. Although phenotypic variability was observed, we identified a male bias for Childhood Apraxia of Speech associated to 16p11.2 microdeletions. We also reported an elevated frequency of second-site genomic alterations, supporting the model of the second hit to explain the clinical variability associated with CNV syndromes. Our goal was to contribute to the building of a chromosome 16 disease-map based on disease susceptibility regions. The role of the CNVs of chromosome 16 was increasingly made clear in the determination of developmental delay. We also found that in some cases a second-site CNV could explain the phenotypic heterogeneity by a simple additive effect or a pejorative synergistic effect.