bluuebunny/crossref_metadata_2025 huggingface dataset:
A Proposal to Improve the Effectiveness of the Deflection Control Method Provided by Eurocodes for Concrete, Timber, and Composite Slabs
Authors: D’Antino Tommaso , Pisani Marco Andrea
Published: December 2021

Limited deflection of structural members represents an important requirement to guarantee proper functionality and appearance of building and infrastructures. According to Eurocodes, this requirement is ensured by limiting the maximum deflection of horizontal structural members to a fraction of their span. However, each Eurocode provides different maximum deflection limits, which are independent of the type of superstructures considered. Thus, the respect of these limits may not always guarantee the integrity of certain superstructures. In this paper, the reliability of the Eurocode deflection control methods, in guaranteeing the integrity of the superstructures, is assessed and discussed. First, different types of horizontal member, namely rib and clay (hollow) pot, composite steel–concrete, and timber beam slabs are designed to respect the deflection limit enforced by the Eurocodes. Then, the maximum curvature developed by these members is compared with the ultimate (limit) curvatures of various superstructures (e.g., ceramic and stone tile floorings). The results obtained show that the approach adopted by Eurocode 2 may provide non-conservative results, but also that the rules proposed by Eurocodes 4 and 5, albeit more reliable, do not always guarantee the integrity of the superstructure. Based on these results, an alternative method, based on the curvature control, is proposed and its advantages and limitations critically discussed. This method appears simpler and more reliable than the method currently adopted by the Eurocodes.
Comparison of the Natural Vibration Frequencies of Timoshenko and Bernoulli Periodic Beams
Authors: Domagalski Łukasz
Published: December 2021

This paper deals with the linear natural vibrations analysis of beams where the geometric and material properties vary periodically along the beam axis. In contrast with homogeneous prismatic beams, the frequency spectra of such beams are irregular as there exist enlarged intervals between some adjacent frequencies. Presented here are two averaged models of beams based on the tolerance modelling approach. The assumptions of classical Euler–Bernoulli and Timoshenko–Ehrenfest beam theories are adopted as the foundations. The resulting mathematical models are systems of differential equations with constant, weight-averaged coefficients. This makes it possible to apply any classical method of solution suitable for homogeneous beams, such as Galerkin orthogonalization. Here, emphasis is placed on the comparison of natural frequencies neighbouring the frequency band-gaps that are obtained from these two theories. Two basic cases of material and geometric property distribution in a periodicity cell are studied, and the natural frequencies and mode shapes are obtained for a simply supported beam. The results are supported by a comparison with the finite element method and partially exact solutions.
Control of Specific/Nonspecific Protein Adsorption: Functionalization of Polyelectrolyte Multilayer Films as a Potential Coating for Biosensors
Authors: Kruk Tomasz , Bzowska Monika , Hinz Alicja , Szuwarzyński Michał , Szczepanowicz Krzysztof
Published: December 2021

Control of nonspecific/specific protein adsorption is the main goal in the design of novel biomaterials, implants, drug delivery systems, and sensors. The specific functionalization of biomaterials can be achieved by proper surface modification. One of the important strategies is covering the materials with functional coatings. Therefore, our work aimed to functionalize multilayer coating to control nonspecific/specific protein adsorption. The polyelectrolyte coating was formed using a layer-by-layer technique (LbL) with biocompatible polyelectrolytes poly-L-lysine hydrobromide (PLL) and poly-L-glutamic acid (PGA). Nonspecific protein adsorption was minimized/eliminated by pegylation of multilayer films, which was achieved by adsorption of pegylated polycations (PLL-g-PEG). The influence of poly (ethylene glycol) chain length on eliminating nonspecific protein adsorption was confirmed. Moreover, to achieve specific protein adsorption, the multilayer film was also functionalized by immobilization of antibodies via a streptavidin bridge. The functional coatings were tested, and the adsorption of the following proteins confirmed the ability to control nonspecific/specific adsorption: human serum albumin (HSA), fibrinogen (FIB), fetal bovine serum (FBS), carcinoembryonic antigen human (CEA) monitored by quartz crystal microbalance with dissipation (QCM-D). AFM imaging of unmodified and modified multilayer surfaces was also performed. Functional multilayer films are believed to have the potential as a novel platform for biotechnological applications, such as biosensors and nanocarriers for drug delivery systems.
Experimental Study of Hardened Young’s Modulus for 3D Printed Mortar
Authors: Skibicki Szymon , Techman Mateusz , Federowicz Karol , Olczyk Norbert , Hoffmann Marcin
Published: December 2021

Few studies have focused on determining the Young’s modulus of 3D printed structures. This study presents the results of experimental investigations of Young’s modulus of a 3D printed mortar. Specimens were prepared in four different ways to investigate possible application of different methods for 3D printed structures. Study determines the influence of the number of layers on mechanical properties of printed samples. Results have shown a strong statistical correlation between the number of layers and value of Young’s modulus. The compressive strength and Young’s modulus reduction compared to standard cylindrical sample were up to 43.1% and 19.8%, respectively. Results of the study shed light on the differences between the current standard specimen used for determination of Young’s modulus and the specimen prepared by 3D printing. The community should discuss the problem of standardization of test methods in view of visible differences between different types of specimens.
An Efficient Computational Model for Magnetic Pulse Forming of Thin Structures
Authors: Mahmoud Mohamed , Bay François , Muñoz Daniel Pino
Published: December 2021

Electromagnetic forming (EMF) is one of the most popular high-speed forming processes for sheet metals. However, modeling this process in 3D often requires huge computational time since it deals with a strongly coupled multi-physics problem. The numerical tools that are capable of modeling this process rely either on shell elements-based approaches or on full 3D elements-based approaches. The former leads to reduced computational time at the expense of the accuracy, while the latter favors accuracy over computation time. Herein, a novel approach was developed to reduce CPU time while maintaining reasonable accuracy through building upon a 3D finite element analysis toolbox which was developed in CEMEF. This toolbox was used to solve magnetic pulse forming (MPF) of thin sheets. The problem was simulated under different conditions and the results were analyzed in-depth. Innovative techniques, such as developing a termination criterion and using adaptive re-meshing, were devised to overcome the encountered problems. Moreover, a solid shell element was implemented and tested for thin structure problems and its applicability was verified. The results of this element type were comparable to the results of the standard tetrahedral MINI element but with reduced simulation time.
A Design Method to Induce Ductile Failure of Flexural Strengthened One-Way RC Slabs
Authors: Nguyen Huy Q. , Nguyen Tri N. M. , Lee Do Hyung , Kim Jung J.
Published: December 2021

Strengthening existing reinforced concrete (RC) slabs using externally bonded materials is increasingly popular due to its adaptability and versatility. Nevertheless, ductility reduction of the rehabilitated flexural members with these materials can lead to brittle shear failure. Therefore, a new approach for strengthening is necessary. This paper presents a methodology to induce ductile failure of flexural strengthened one-way RC slabs. Ultimate failure loads can be considered to develop the proposed design methodology. Different failure modes corresponding to ultimate failure loads for RC slabs are addressed. Flexural and shear failure regions of RC slabs can be established by considering the failure modes. The end span of the concrete slab is shown for a case study, and numerical examples are solved to prove the essentiality of this methodology.
Probing the Use of Silane-Grafted Fumed Silica Nanoparticles to Produce Stable Transformer Oil-Based Nanofluids
Authors: Qureshi Muhammad I. , Qureshi Basit
Published: December 2021

In this experimental investigation, hydrophobic silane-grafted fumed nano-silica was employed in transformer oil to formulate nanofluids (NFs). A cold-air atmosphere-pressure plasma reactor working on the principle of dielectric barrier discharge was designed and utilized to functionalize the surface of these nanoparticles. A field emission scanning electron microscope (FE-SEM) coupled with energy-dispersive X-ray (EDX) module and Fourier transform infrared (FTIR) spectroscopy were used to scan surface features of new and plasma-treated nanoparticles. The study revealed considerable changes in the surface chemistry of nanoparticles, which led to good dispersibility and stability of nanofluids. The measurements of AC breakdown voltages (AC-BDV) of nanofluids so prepared were conducted according to IEC-Std 60156, and a significant improvement in the dielectric strength was achieved. A statistical analysis of these results was performed using Weibull probabilistic law. At a 5% probability of failure, modified nanofluid remarkably exhibited a 60% increase in breakdown voltage. The dielectric properties such as variation of εr and tan δ in temperature of up to 70 °C were measured and compared with untreated fluid. Results exhibit an increase in tan δ and a slight decrease in permittivity of nanofluids. The analysis also revealed that while unpolar silane coating of NPs increased the breakdown strength, the polar-amino-silane-coated NPs in oil resulted in a drastic reduction. Details of this antagonistic trend are elaborated in this paper.
Effect of Conventional Adhesive Application or Co-Curing Technique on Dentin Bond Strength
Authors: Vukelja Josipa , Klarić Sever Eva , Sever Ivan , Jukić Krmek Silvana , Tarle Zrinka
Published: December 2021

The aim of this in vitro study was to assess the effect of two different adhesive application methods on shear dentin bond strength (ISO 29022) using three various adhesive systems. A mid-coronal section of 77 intact third human molars with fully developed apices was made to create flat bonding substrates. The materials used in the study were Excite F (Ivoclar Vivadent), Prime&Bond Universal (Dentsply Sirona) and G-Premio Bond (GC). The application of each adhesion system was performed in two different ways. In the first group, the bonding agent was light cured immediately after the application (conventional method), while in the second group the adhesive and composite were cured concurrently (“co-curing” method). A total of 180 specimens were prepared (3 adhesives × 2 method of application × 30 specimens per experimental group), stored at 37 °C in distilled water and fractured in shear mode after 1 week. Statistical analysis was performed using ANOVA and Weibull statistics. The highest bond strength was obtained for Prime&Bond conventional (21.7 MPa), whilst the lowest bond strength was observed when co-curing was used (particularly, Excite F 12.2 MPa). The results showed a significant difference between conventional and co-curing methods in all materials. According to reliability analysis, the co-curing method diminished bond reliability. Different application techniques exhibit different bond strengths to dentin.
Mixed Manganese Dioxide on Magnetite Core MnO2@Fe3O4 as a Filler in a High-Performance Magnetic Alginate Membrane
Authors: Grzybek Paweł , Turczyn Roman , Dudek Gabriela
Published: December 2021

The process of ethanol dehydration via pervaporation was performed using alginate membranes filled with manganese dioxide and a mixed filler consisting of manganese dioxide on magnetite core MnO2@Fe3O4 particles. The crystallization of manganese dioxide on magnetite nanoparticle surface resulted in a better dispersibility of this mixed filler in polymer matrix, with the preservation of the magnetic properties of magnetite. The prepared membranes were characterized by contact angle, degree of swelling and SEM microscopy measurements and correlated with their effectiveness in the pervaporative dehydration of ethanol. The results show a strong relation between filler properties and separation efficiency. The membranes filled with the mixed filler outperformed the membranes containing only neat oxide, exhibiting both higher flux and separation factor. The performance changed depending on filler content; thus, the presence of optimum filler loading was observed for the studied membranes. The best results were obtained for the alginate membrane filled with 7 wt.% of mixed filler MnO2@Fe3O4 particles. For this membrane, the separation factor and flux equalled to 483 and 1.22 kg·m−2·h−1, respectively.
Hesitant Fuzzy 2-Dimension Linguistic Programming Technique for Multidimensional Analysis of Preference for Multicriteria Group Decision Making
Authors: Liu Xiaoyue , Ju Dawei
Published: December 2021

The hesitant fuzzy 2-dimension linguistic element (HF2DLE) allows decision makers to express the importance or reliability of each term included in a hesitant fuzzy linguistic element as a linguistic term. This paper investigates a programming technique for multidimensional analysis of preference for hesitant fuzzy 2-dimension linguistic multicriteria group decision making. Considering the flexibility of HF2DLEs in expressing hesitant qualitative preference information, we first adopt HF2DLEs to depict both the evaluation values of alternatives and the truth degrees of alternative comparisons. To calculate the relative closeness degrees (RCDs) of alternatives, the Euclidean distances between HF2DLEs are defined. Based on RCDs and preference relations on alternatives, the group consistency and inconsistency indices are constructed, and a bi-objective hesitant fuzzy 2-dimension linguistic programming model is established to derive the criteria weights and positive and negative ideal solutions. Since the objective functions and partial constraint coefficients of the established model are HF2DLEs, an effective solution is developed, through which the RCDs can be calculated to obtain the individual rankings of alternatives. Furthermore, a single-objective assignment model is constructed to determine the best alternative. Finally, a case study is conducted to illustrate the feasibility of the proposed method, and its effectiveness is demonstrated by comparison analyses.
Design and Fabrication of Low-Cost Microfluidic Chips and Microfluidic Routing System for Reconfigurable Multi-(Organ-on-a-Chip) Assembly
Authors: Abu-Dawas Sadeq , Alawami Hawra , Zourob Mohammed , Ramadan Qasem
Published: December 2021

A low-cost, versatile, and reconfigurable fluidic routing system and chip assembly have been fabricated and tested. The platform and its accessories were fabricated in-house without the need for costly and specialized equipment nor specific expertise. An agarose-based artificial membrane was integrated into the chips and employed to test the chip-to-chip communication in various configurations. Various chip assemblies were constructed and tested which demonstrate the versatile utility of the fluidic routing system that enables the custom design of the chip-to-chip communication and the possibility of fitting a variety of (organ-on-a-chip)-based biological models with multicell architectures. The reconfigurable chip assembly would enable selective linking/isolating the desired chip/compartment, hence allowing the study of the contribution of specific cell/tissue within the in vitro models.
Vibration Energy Harvester Based on Torsionally Oscillating Magnet
Authors: Wang Xinyi , Li Jiaxing , Zhou Chenyuan , Tao Kai , Qiao Dayong , Li Yunjia
Published: December 2021

Most of the miniaturized electromagnetic vibrational energy harvesters (EVEHs) are based on oscillating proof mass suspended by several springs or a cantilever structure. Such structural feature limits the miniaturization of the device’s footprint. This paper presents an EVEH device based on a torsional vibrating magnet over a stack of flexible planar coils. The torsional movement of the magnet is enabled by microfabricated silicon torsional springs, which effectively reduce the footprint of the device. With a size of 1 cm × 1 cm × 1.08 cm, the proposed EVEH is capable of generating an open-circuit peak-to-peak voltage of 169 mV and a power of 6.9 μW, under a sinusoidal excitation of ±0.5 g (g = 9.8 m/s2) and frequency of 96 Hz. At elevated acceleration levels, the maximum peak-to-peak output voltage is 222 mV under the acceleration of 7 g (±3.5 g).
Study on the Mechanism of Solid-Phase Oxidant Action in Tribochemical Mechanical Polishing of SiC Single Crystal Substrate
Authors: Qi Wanting , Cao Xiaojun , Xiao Wen , Wang Zhankui , Su Jianxiu
Published: December 2021

Na2CO3—1.5 H2O2, KClO3, KMnO4, KIO3, and NaOH were selected for dry polishing tests with a 6H-SiC single crystal substrate on a polyurethane polishing pad. The research results showed that all the solid-phase oxidants, except NaOH, could decompose to produce oxygen under the frictional action. After polishing with the five solid-phase oxidants, oxygen was found on the surface of SiC, indicating that all five solid-phase oxidants can have complex tribochemical reactions with SiC. Their reaction products are mainly SiO2 and (SiO2)x. Under the action of friction, due to the high flash point temperature of the polishing interface, the oxygen generated by the decomposition of the solid-phase oxidant could oxidize the surface of SiC and generate a SiO2 oxide layer on the surface of SiC. On the other hand, SiC reacted with H2O and generated a SiO2 oxide layer on the surface of SiC. After polishing with NaOH, the SiO2 oxide layer and soluble Na2SiO3 could be generated on the SiC surface; therefore, the surface material removal rate (MRR) was the highest, and the surface roughness was the largest, after polishing. The lowest MRR was achieved after the dry polishing of SiC with KClO3.
The Analgesic Properties of Corydalis yanhusuo
Authors: Alhassen Lamees , Dabbous Travis , Ha Allyssa , Dang Leon Hoang Lam , Civelli Olivier
Published: December 2021

Corydalis yanhusuo extract (YHS) has been used for centuries across Asia for pain relief. The extract is made up of more than 160 compounds and has been identified as alkaloids, organic acids, volatile oils, amino acids, alcohols, and sugars. However, the most crucial biological active constituents of YHS are alkaloids; more than 80 have been isolated and identified. This review paper aims to provide a comprehensive review of the phytochemical and pharmacological effects of these alkaloids that have significant ties to analgesia.
Correlative Analysis of the Dimensional Properties of Bipyramidal Titania Nanoparticles by Complementing Electron Microscopy with Other Methods
Authors: Crouzier Loïc , Feltin Nicolas , Delvallée Alexandra , Pellegrino Francesco , Maurino Valter , Cios Grzegorz , Tokarski Tomasz , Salzmann Christoph , Deumer Jérôme , Gollwitzer Christian , Hodoroaba Vasile-Dan
Published: December 2021

In this paper, the accurate determination of the size and size distribution of bipyramidal anatase nanoparticles (NPs) after deposition as single particles on a silicon substrate by correlative Scanning Electron Microscopy (SEM) with Atomic Force Microscopy (AFM) analysis is described as a new measurement procedure for metrological purposes. The knowledge of the exact orientation of the NPs is a crucial step in extracting the real 3D dimensions of the particles. Two approaches are proposed to determine the geometrical orientation of individual nano-bipyramides: (i) AFM profiling along the long bipyramid axis and (ii) stage tilting followed by SEM imaging. Furthermore, a recently developed method, Transmission Kikuchi Diffraction (TKD), which needs preparation of the crystalline NPs on electron-transparent substrates such as TEM grids, has been tested with respect to its capability of identifying the geometrical orientation of the individual NPs. With the NPs prepared homogeneously on a TEM grid, the transmission mode in a SEM, i.e., STEM-in-SEM (or T-SEM), can be also applied to extract accurate projection dimensions of the nanoparticles from the same sample area as that analysed by SEM, TKD and possibly AFM. Finally, Small Angle X-ray Scattering (SAXS) can be used as an ensemble technique able to measure the NPs in liquid suspension and, with ab-initio knowledge of the NP shape from the descriptive imaging techniques, to provide traceable NP size distribution and particle concentration.
Double Narrow Fano Resonances via Diffraction Coupling of Magnetic Plasmon Resonances in Embedded 3D Metamaterials for High-Quality Sensing
Authors: Hu Haitao , Lu Xue , Huang Jianhua , Chen Kai , Su Jun , Yan Zhendong , Tang Chaojun , Cai Pingen
Published: December 2021

We theoretically demonstrate an approach to generate the double narrow Fano resonances via diffraction coupling of magnetic plasmon (MP) resonances by embedding 3D metamaterials composed of vertical Au U-shaped split-ring resonators (VSRRs) array into a dielectric substrate. Our strategy offers a homogeneous background allowing strong coupling between the MP resonances of VSRRs and the two surface collective optical modes of a periodic array resulting from Wood anomaly, which leads to two narrow hybridized MP modes from the visible to near-infrared regions. In addition, the interaction effects in the VSRRs with various geometric parameters are also systematically studied. Owing to the narrow hybrid MP mode being highly sensitive to small changes in the surrounding media, the sensitivity and the figure of merit (FoM) of the embedded 3D metamaterials with fabrication feasibility were as high as 590 nm/RIU and 104, respectively, which holds practical applications in label-free biosensing, such as the detection of medical diagnoses and sport doping drugs.
Thermoelectric Transport in a Three-Dimensional HgTe Topological Insulator
Authors: Gusev Gennady M. , Kvon Ze D. , Levin Alexander D. , Mikhailov Nikolay N.
Published: December 2021

The thermoelectric response of 80 nm-thick strained HgTe films of a three-dimensional topological insulator (3D TI) has been studied experimentally. An ambipolar thermopower is observed where the Fermi energy moves from conducting to the valence bulk band. The comparison between theory and experiment shows that the thermopower is mostly due to the phonon drag contribution. In the region where the 2D Dirac electrons coexist with bulk hole states, the Seebeck coefficient is modified due to 2D electron–3D hole scattering.
Graded Morphologies and the Performance of PffBT4T-2OD:PC71BM Devices Using Additive Choice
Authors: Gaspar Hugo , Parnell Andrew J. , Pérez Gabriel E. , Viana Júlio C. , King Stephen M. , Mendes Adélio , Pereira Luiz , Bernardo Gabriel
Published: December 2021

The impact of several solvent processing additives (1-chloronaphthalene, methylnaphthalene, hexadecane, 1-phenyloctane, and p-anisaldehyde), 3% v/v in o-dichlorobenzene, on the performance and morphology of poly[(5,6-difluoro-2,1,3-benzothiadiazol-4,7-diyl)-alt-(3,3‴-di(2-octyldodecyl)-2,2′,5′,22033,5″,2‴-quaterthiophen-5,5‴-diyl)] (PffBT4T-2OD):[6,6]-phenyl-C71-butyric acid methyl ester (PC71BM)-based polymer solar cells was investigated. Some additives were shown to enhance the power conversion efficiency (PCE) by ~6%, while others decreased the PCE by ~17–25% and a subset of the additives tested completely eliminated any power conversion efficiency and the operation as a photovoltaic device. Grazing-Incidence Wide Angle X-ray Scattering (GIWAXS) revealed a clear stepwise variation in the crystallinity of the systems when changing the additive between the two extreme situations of maximum PCE (1-chloronaphthalene) and null PCE (hexadecane). Small-Angle Neutron Scattering (SANS) revealed that the morphology of devices with PCE ~0% was composed of large domains with correlation lengths of ~30 nm, i.e., much larger than the typical exciton diffusion length (~12 nm) in organic semiconductors. The graded variations in crystallinity and in nano-domain size observed between the two extreme situations (1-chloronaphthalene and hexadecane) were responsible for the observed graded variations in device performance.
Heat-Up Colloidal Synthesis of Shape-Controlled Cu-Se-S Nanostructures—Role of Precursor and Surfactant Reactivity and Performance in N2 Electroreduction
Authors: Mourdikoudis Stefanos , Antonaropoulos George , Antonatos Nikolas , Rosado Marcos , Storozhuk Liudmyla , Takahashi Mari , Maenosono Shinya , Luxa Jan , Sofer Zdeněk , Ballesteros Belén , Thanh Nguyen Thi Kim , Lappas Alexandros
Published: December 2021

Copper selenide-sulfide nanostructures were synthesized using metal-organic chemical routes in the presence of Cu- and Se-precursors as well as S-containing compounds. Our goal was first to examine if the initial Cu/Se 1:1 molar proportion in the starting reagents would always lead to equiatomic composition in the final product, depending on other synthesis parameters which affect the reagents reactivity. Such reaction conditions were the types of precursors, surfactants and other reagents, as well as the synthesis temperature. The use of ‘hot-injection’ processes was avoided, focusing on ‘non-injection’ ones; that is, only heat-up protocols were employed, which have the advantage of simple operation and scalability. All reagents were mixed at room temperature followed by further heating to a selected high temperature. It was found that for samples with particles of bigger size and anisotropic shape the CuSe composition was favored, whereas particles with smaller size and spherical shape possessed a Cu2−xSe phase, especially when no sulfur was present. Apart from elemental Se, Al2Se3 was used as an efficient selenium source for the first time for the acquisition of copper selenide nanostructures. The use of dodecanethiol in the presence of trioctylphosphine and elemental Se promoted the incorporation of sulfur in the materials crystal lattice, leading to Cu-Se-S compositions. A variety of techniques were used to characterize the formed nanomaterials such as XRD, TEM, HRTEM, STEM-EDX, AFM and UV-Vis-NIR. Promising results, especially for thin anisotropic nanoplates for use as electrocatalysts in nitrogen reduction reaction (NRR), were obtained.
Positron Annihilation Lifetime Spectroscopy Insight on Free Volume Conversion of Nanostructured MgAl2O4 Ceramics
Authors: Klym Halyna , Karbovnyk Ivan , Piskunov Sergei , Popov Anatoli I.
Published: December 2021

Herein we demonstrate the specifics of using the positron annihilation lifetime spectroscopy (PALS) method for the study of free volume changes in functional ceramic materials. Choosing technological modification of nanostructured MgAl2O4 spinel as an example, we show that for ceramics with well-developed porosity positron annihilation is revealed through two channels: positron trapping channel and ortho-positronium decay. Positron trapping in free-volume defects is described by the second component of spectra and ortho-positronium decay process by single or multiple components, depending on how well porosity is developed and on the experimental configuration. When using proposed positron annihilation lifetime spectroscopy approaches, three components are the most suitable fit in the case of MgAl2O4 ceramics. In the analysis of the second component, it is shown that technological modification (increasing sintering temperature) leads to volume shrinking and decreases the number of defect-related voids. This process is also accompanied by the decrease of the size of nanopores (described by the third component), while the overall number of nanopores is not affected. The approach to the analysis of positron annihilation lifetime spectra presented here can be applied to a wide range of functional nanomaterials with pronounced porosity.
Pancreatic Cancer and Gut Microbiome-Related Aspects: A Comprehensive Review and Dietary Recommendations
Authors: Sobocki Bartosz Kamil , Kaźmierczak-Siedlecka Karolina , Folwarski Marcin , Hawryłkowicz Viktoria , Makarewicz Wojciech , Stachowska Ewa
Published: December 2021

Gut microbiota plays a significant role in the human body providing many beneficial effects on the host. However, its dysbiotic alterations may affect the tumorigenic pathway and then trigger the development of pancreatic cancer. This dysbiosis can also modulate the aggressiveness of the tumor, influencing the microenvironment. Because pancreatic cancer is still one of the most lethal cancers worldwide with surgery as the only method that influences prognosis and has curative potential, there is a need to search for other strategies which will enhance the efficiency of standard therapy and improve patients’ quality of life. The administration of prebiotics, probiotics, next-generation probiotics (Faecalibacterium prausnitzii, Akkermansia muciniphila), synbiotics, postbiotics, and fecal microbiota transplantation through multiple mechanisms affects the composition of the gut microbiota and may restore its balance. Despite limited data, some studies indicate that the aforementioned methods may allow to achieve better effect of pancreatic cancer treatment and improve therapeutic strategies for pancreatic cancer patients.
Nutrients to Improve Mitochondrial Function to Reduce Brain Energy Deficit and Oxidative Stress in Migraine
Authors: Fila Michal , Chojnacki Cezary , Chojnacki Jan , Blasiak Janusz
Published: December 2021

The mechanisms of migraine pathogenesis are not completely clear, but 31P-nuclear magnetic resonance studies revealed brain energy deficit in migraineurs. As glycolysis is the main process of energy production in the brain, mitochondria may play an important role in migraine pathogenesis. Nutrition is an important aspect of migraine pathogenesis, as many migraineurs report food-related products as migraine triggers. Apart from approved anti-migraine drugs, many vitamins and supplements are considered in migraine prevention and therapy, but without strong supportive evidence. In this review, we summarize and update information about nutrients that may be important for mitochondrial functions, energy production, oxidative stress, and that are related to migraine. Additionally, we present a brief overview of caffeine and alcohol, as they are often reported to have ambiguous effects in migraineurs. The nutrients that can be considered to supplement the diet to prevent and/or ameliorate migraine are riboflavin, thiamine, magnesium ions, niacin, carnitine, coenzyme Q10, melatonin, lipoic acid, pyridoxine, folate, and cobalamin. They can supplement a normal, healthy diet, which should be adjusted to individual needs determined mainly by the physiological constitution of an organism. The intake of caffeine and alcohol should be fine-tuned to the history of their use, as withdrawal of these agents in regular users may become a migraine trigger.
Effect of Black Tea Consumption on Urinary Risk Factors for Kidney Stone Formation
Authors: Siener Roswitha , Hesse Albrecht
Published: December 2021

Copious fluid intake is the most essential nutritional measure in the treatment of urolithiasis, and is suggested to be a protective factor in the primary prevention of urinary stone formation. Although the intake of black tea contributes to daily fluid intake, the high oxalate content could outweigh the beneficial effect of urine dilution. The present study investigated the effect of black tea consumption on urinary risk factors for kidney stone formation. Ten healthy men received a standardized diet for a period of ten days. Subjects consumed 1.5 L/day of fruit tea (0 mg/day oxalate) during the 5-day control phase, which was replaced by 1.5 L/day of black tea (86 mg/day oxalate) during the 5-day test phase. Fractional and 24-h urines were obtained. The intake of black tea did not significantly alter 24-h urinary oxalate excretion. Urinary citrate, an important inhibitor of calcium stone formation, increased significantly, while the relative supersaturation of calcium oxalate, uric acid, and struvite remained unchanged. No significantly increased risk for kidney stone formation could be derived from the ingestion of black tea in normal subjects. Further research is needed to evaluate the impact of black tea consumption in kidney stone patients with intestinal hyperabsorption of oxalate.
Dietary Intake of Advanced Glycation End Products (AGEs) and Mortality among Individuals with Colorectal Cancer
Authors: Mao Ziling , Aglago Elom K. , Zhao Zhiwei , Schalkwijk Casper , Jiao Li , Freisling Heinz , Weiderpass Elisabete , Hughes David J. , Eriksen Anne Kirstine , Tjønneland Anne , Severi Gianluca , Rothwell Joseph , Boutron-Ruault Marie-Christine , Katzke Verena , Kaaks Rudolf , Schulze Matthias B. , Birukov Anna , Krogh Vittorio , Panico Salvatore , Tumino Rosario , Ricceri Fulvio , Bueno-de-Mesquita H. Bas , Vermeulen Roel C. H. , Gram Inger T. , Skeie Guri , Sandanger Torkjel M. , Quirós J. Ramón , Crous-Bou Marta , Sánchez Maria-Jose , Amiano Pilar , Chirlaque María-Dolores , Barricarte Gurrea Aurelio , Manjer Jonas , Johansson Ingegerd , Perez-Cornago Aurora , Jenab Mazda , Fedirko Veronika
Published: December 2021

Advanced glycation end-products (AGEs) may promote oxidative stress and inflammation and have been linked to multiple chronic diseases, including cancer. However, the association of AGEs with mortality after colorectal cancer (CRC) diagnosis has not been previously investigated. Multivariable Cox proportional hazards models were used to calculate hazard ratios and corresponding 95% confidence intervals for associations between dietary intake of AGEs with CRC-specific and all-cause mortality among 5801 participant cases diagnosed with CRC in the European Prospective Investigation into Cancer and Nutrition study between 1993 and 2013. Dietary intakes of AGEs were estimated using country-specific dietary questionnaires, linked to an AGE database, that accounted for food preparation and processing. During a median of 58 months of follow-up, 2421 cases died (1841 from CRC). Individually or combined, dietary intakes of AGEs were not associated with all-cause and CRC-specific mortality among cases. However, there was a suggestion for a positive association between AGEs and all-cause or CRC-specific mortality among CRC cases without type II diabetes (all-cause, Pinteraction = 0.05) and CRC cases with the longest follow-up between recruitment and cancer diagnosis (CRC-specific, Pinteraction = 0.003; all-cause, Pinteraction = 0.01). Our study suggests that pre-diagnostic dietary intakes of AGEs were not associated with CRC-specific or all-cause mortality among CRC patients. Further investigations using biomarkers of AGEs and stratifying by sex, diabetes status, and timing of exposure to AGEs are warranted.
Protective Effect of Lactiplantibacillus plantarum 1201 Combined with Galactooligosaccharide on Carbon Tetrachloride-Induced Acute Liver Injury in Mice
Authors: Ren Zhongyue , Huo Yalan , Zhang Qimeng , Chen Shufang , Lv Huihui , Peng Lingling , Wei Hua , Wan Cuixiang
Published: December 2021

Acute liver injury (ALI) has a high mortality rate of approximately 20–40%, and it is imperative to find complementary and alternative drugs for treating ALI. A carbon tetrachloride (CCl4)-induced ALI mouse model was established to explore whether dietary intervention can alleviate ALI in mice. Intestinal flora, intestinal integrity, biomarkers of hepatic function, systemic inflammation, autophagy, and apoptosis signals were detected through a real-time PCR, hematoxylin-eosin staining, 16S rRNA gene sequencing, and so on. The results showed that Lactiplantibacillus plantarum 1201 had a strongly antioxidant ability, and galactooligosaccharide (GOS) could boost its growth. Based on these findings, the combination of L. plantarum 1201 and GOS, the synbiotic, was applied to prevent CCl4-induced ALI in mice. The current research proved that GOS promoted the intestinal colonization of L. plantarum 1201, and the synbiotic improved the antioxidant capacity of the host, regulated the intestinal flora, repaired the intestinal barrier, inhibited the activation of the MAPK/NF-κB pathway, and then inhibited the apoptosis and autophagy pathways, relieving inflammation and liver oxidation; thereby, the ALI of mice was alleviated. These results suggest that synbiotics may become a new research direction for liver-protecting drugs.
The Impact of Household Chaos and Dietary Intake on Executive Function in Young Children
Authors: Iwinski Samantha , Donovan Sharon M. , Fiese Barbara , Bost Kelly
Published: December 2021

Children’s executive functions (EFs) emerge over time and can be shaped by household environments and dietary intake. However, there is a lack of knowledge about how these factors influence EFs in children aged 18–24 months. This study tested a model exploring the relations between parent-reported dietary intake, household chaos, and child EF. The sample consisted of 294 families participating in the STRONG Kids2 birth cohort study of nutrition and child health. Caregivers completed the Food Frequency Questionnaire (FFQ), the Confusion, Hubbub, and Order Scale (CHAOS), and the Behavior Rating Inventory of Executive Function®-Preschool Version (BRIEF-P) to assess model variables. Regression analyses revealed a significant and independent association between assorted snacks and processed foods and two EF subscales. There were also significant associations between household chaos and each EF subscale. There was no significant moderation effect. These findings suggest that family households characterized by dysregulation are associated with children’s EF difficulties during early childhood and that the role of unhealthy dietary intake in child EF should be explored further. Future longitudinal studies that include multi-method approaches are needed to document the mechanisms through which household chaos impacts child EF over time.
Association of Urinary Potassium Excretion with Blood Pressure Variability and Cardiovascular Outcomes in Patients with Pre-Dialysis Chronic Kidney Disease
Authors: Suh Sang Heon , Song Su Hyun , Oh Tae Ryom , Choi Hong Sang , Kim Chang Seong , Bae Eun Hui , Oh Kook-Hwan , Lee Joongyub , Han Seung Hyeok , Kim Yeong Hoon , Chae Dong-Wan , Ma Seong Kwon , Kim Soo Wan ,   on behalf of the Korean Cohort Study for Outcomes in Patients with Chronic Kidney Disease (KNOW-CKD) Investigators
Published: December 2021

Dietary potassium intake is a dilemma in patients with chronic kidney disease (CKD). We investigated the association of urine potassium excretion, a surrogate for dietary potassium intake, with blood pressure variability (BPV) and cardiovascular (CV) outcomes in patients with pre-dialysis CKD. A total of 1860 participants from a cohort of pre-dialysis CKD (KNOW-CKD) patients were divided into the quartiles by spot urine potassium-to-creatinine ratio. The first quartile (26.423 ± 5.731 mmol/gCr) was defined as low urine potassium excretion. Multivariate linear regression analyses revealed an independent association of low urine potassium excretion with high BPV (adjusted β coefficient 1.163, 95% confidence interval 0.424 to 1.901). Cox regression analyses demonstrated that, compared to high urine potassium excretion, low urine potassium excretion is associated with increased risk of CV events (adjusted hazard ratio 2.502, 95% confidence interval 1.162 to 5.387) but not with all-cause mortality. In conclusion, low urine potassium excretion is associated with high BPV and increased risk of CV events in patients with pre-dialysis CKD. The restriction of dietary potassium intake should be individualized in patients with pre-dialysis CKD.
Decoding the Oncogenic Signals from the Long Non-Coding RNAs
Authors: Nadhan Revathy , Dhanasekaran Danny N.
Published: December 2021

Cancer is one of the leading causes of death worldwide. Multifactorial etiology of cancer and tumor heterogeneity are the two most acute challenges in existing diagnostic and therapeutic strategies for cancer. An effective precision cancer medicine strategy to overcome these challenges requires a clear understanding of the transcriptomic landscape of cancer cells. Recent innovative breakthroughs in high-throughput sequencing technologies have identified the oncogenic or tumor-suppressor role of several long non-coding RNAs (lncRNAs). LncRNAs have been characterized as regulating various signaling cascades which are involved in the pathobiology of cancer. They modulate cancer cell survival, proliferation, metabolism, invasive metastasis, stemness, and therapy-resistance through their interactions with specific sets of proteins, miRNAs and other non-coding RNAs, mRNAs, or DNAs in cells. By virtue of their ability to regulate multiple sets of genes and their cognate signaling pathways, lncRNAs are emerging as potential candidates for diagnostic, prognostic, and therapeutic targets. This review is focused on providing insight into the mechanisms by which different lncRNAs play a critical role in cancer growth, and their potential role in cancer diagnosis, prognosis, and therapy.
Probing Allosteric Hsp70 Inhibitors by Molecular Modelling Studies to Expedite the Development of Novel Combined F508del CFTR Modulators
Authors: Sabbadini Roberto , Pesce Emanuela , Parodi Alice , Mustorgi Eleonora , Bruzzone Santina , Pedemonte Nicoletta , Casale Monica , Millo Enrico , Cichero Elena
Published: December 2021

Cystic fibrosis (CF) is caused by different mutations related to the cystic fibrosis transmembrane regulator protein (CFTR), with F508del being the most common. Pioneering the development of CFTR modulators, thanks to the development of effective correctors or potentiators, more recent studies deeply encouraged the administration of triple combination therapeutics. However, combinations of molecules interacting with other proteins involved in functionality of the CFTR channel recently arose as a promising approach to address a large rescue of F508del-CFTR. In this context, the design of compounds properly targeting the molecular chaperone Hsp70, such as the allosteric inhibitor MKT-077, proved to be effective for the development of indirect CFTR modulators, endowed with ability to amplify the accumulation of the rescued protein. Herein we performed structure-based studies of a number of allosteric HSP70 inhibitors, considering the recent X-ray crystallographic structure of the human enzyme. This allowed us to point out the main interaction supporting the binding mode of MKT-077, as well as of the related analogues. In particular, cation-π and π–π stacking with the conserve residue Tyr175 deeply stabilized inhibitor binding at the HSP70 cavity. Molecular docking studies had been followed by QSAR analysis and then by virtual screening of aminoaryl thiazoles (I–IIIa) as putative HSP70 inhibitors. Their effectiveness as CFTR modulators has been verified by biological assays, in combination with VX-809, whose positive results confirmed the reliability of the whole applied computational method. Along with this, the “in-silico” prediction of absorption, distribution, metabolism, and excretion (ADME) properties highlighted, once more, that AATs may represent a chemical class to be further investigated for the rational design of novel combination of compounds for CF treatment.
Biodiversity of Root Endophytic Fungi from Oxyria sinensis Grown in Metal-Polluted and Unpolluted Soils in Yunnan Province, Southwestern China
Authors: Zhu Meiyan , Ding Yanhua , Li Xuejiao , Xiao Yuqing , Zhao Zhiwei , Li Tao
Published: December 2021

Oxyria sinensis adopts a tolerant strategy as a metal excluder to survive toxic metal concentrations. Biodiversity and the endophytic fungal community colonizing the O. sinensis roots were assessed from a mining area (MA) and a neighboring non-mining area (nMA) in southwestern China. All O. sinensis roots formed fully developed dark septate endophytes (DSEs) and arbuscular mycorrhizal fungi (AMF). Total DSE colonization was higher for the MA versus nMA, in contrast to the total AMF colonization in the two sites. The DSE colonization was higher than AMF colonization regardless of the site. Pure-culture data showed that the fungi closely related to Exophiala, Cadophora and Phialophora dominantly colonized the O. sinensis roots. A total of 450 operational taxonomic units (OTUs) were identified showing the presence of a distinct fungal community in MA and nMA, which was shaped by soil physiochemical properties, including soil Zn concentrations and organic matter. We found that O. sinensis accumulates and adapts efficiently to local endophytic fungi to achieve the expansion of its community, including the spontaneously reclaimed DSE. This property may be targeted to achieve its colonization with a pioneer plant for phytoremediation in the restoration of a vegetation cover in a metal-contaminated area.
Mechanically Reinforced, Flexible, Hydrophobic and UV Impermeable Starch-Cellulose Nanofibers (CNF)-Lignin Composites with Good Barrier and Thermal Properties
Authors: Zhao Yadong , Troedsson Christofer , Bouquet Jean-Marie , Thompson Eric M. , Zheng Bin , Wang Miao
Published: December 2021

Bio-based composite films have been widely studied as potential substitutes for conventional plastics in food packaging. The aim of this study was to develop multifunctional composite films by introducing cellulose nanofibers (CNF) and lignin into starch-based films. Instead of costly and complicated chemical modification or covalent coupling, this study optimized the performance of the composite films by simply tuning the formulation. We found that starch films were mechanically reinforced by CNF, with lignin dispersing as nanoparticles embedded in the matrix. The newly built-up hydrogen bonding between these three components improves the integration of the films, while the introduction of CNF and lignin improved the thermal stability of the starch-based films. Lignin, as a functional additive, improved hydrophobicity and blocked UV transmission. The inherent barrier property of CNF and the dense starch matrix provided the composite films with good gas barrier properties. The prepared flexible films were optically transparent, and exhibited UV blocking ability, good oxygen-barrier properties, high hydrophobicity, appreciable mechanical strength and good thermal stability. These characteristics indicate potential utilization as a green alternative to synthetic plastics especially for food packaging applications.