bluuebunny/crossref_metadata_2025 huggingface dataset:
Container Volume Prediction Using Time-Series Decomposition with a Long Short-Term Memory Models
Authors: Lee Eunju , Kim Dohee , Bae Hyerim
Published: September 2021

The purpose of this study is to improve the prediction of container volumes in Busan ports by applying external variables and time-series data decomposition methods to deep learning prediction models. Previous studies on container volume forecasting were based on traditional statistical methodologies, such as ARIMA, SARIMA, and regression. However, these methods do not explain the complexity and variability of data caused by changes in the external environment, such as the global financial crisis and economic fluctuations. Deep learning can explore the inherent patterns of data and analyze the characteristics (time series, external environmental variables, and outliers); hence, the accuracy of deep learning-based volume prediction models is better than that of traditional models. However, this does not include the study of overall trends (upward, steady, or downward). In this study, a novel deep learning prediction model is proposed that combines prediction and trend identification of container volume. The proposed model explores external variables that are related to container volume, combining port volume time-series decomposition with external variables and deep learning-based multivariate long short-term memory (LSTM) prediction. The results indicate that the proposed model performs better than the traditional LSTM model and follows the trend simultaneously.
A Comparative Study of Cerium- and Ytterbium-Based GO/g-C3N4/Fe2O3 Composites for Electrochemical and Photocatalytic Applications
Authors: Farooq Nosheen , Luque Rafael , Hessien Mahmoud M. , Qureshi Ashfaq Mahmood , Sahiba Farzana , Nazir Muhammad Altaf , ur Rehman Aziz
Published: September 2021

The design of sustainable and efficient materials for efficient energy storage and degradation of environmental pollutants (specifically organic dyes) is a matter of major interest these days. For this purpose, cerium- and ytterbium-based GO/g-C3N4/Fe2O3 composites have been synthesized to explore their properties, especially in charge storage devices such as supercapacitors, and also as photocatalysts for the degradation of carcinogenic dyes from the environment. Physicochemical studies have been carried out using XRD, FTIR, SEM, and BET techniques. Electrochemical techniques (cyclic voltammetry, galvanic charge discharge, and electrochemical impedance spectroscopy) have been employed to measure super-capacitance and EDLC properties. Results show that the gravimetric capacitance calculated from GCD results is 219 Fg−1 for ytterbium- and 169 Fg−1 for cerium-based nanocomposites at the current density of 1 A/g and scan rate of 2 mV/sec. The specific capacitance calculated for the ytterbium-based nanocomposite is 189 Fg−1 as compared to 125 Fg−1 for the cerium-based material. EIS results pointed to an enhanced resistance offered by cerium-based nanocomposites as compared to that of ytterbium, which can be assumed with the difference in particle size, as confirmed from structural studies including XRD. From obtained results, ytterbium oxide-based GO/g-C3N4/Fe2O3 is proven to be a better electro-catalyst as compared to cerium-based nanocomposites. Photocatalytic results are also in agreement with electrochemical results, as the degradation efficiency of ytterbium oxide-based GO/g-C3N4/Fe2O3 (67.11 and 83.50% for rhodamine B and methylene blue dyes) surpasses values observed for cerium-based GO/g-C3N4/Fe2O3 (63.08 and 70.61%).
Measurement of CO2 by Wavelength Modulated Reinjection Off-Axis Integrated Cavity Output Spectroscopy at 2 μm
Authors: Yuan Zihao , Huang Yinbo , Lu Xingji , Huang Jun , Liu Qiang , Qi Gang , Cao Zhensong
Published: September 2021

A high sensitivity wavelength modulated reinjection off-axis integrated cavity output spectroscopy (WM-RE-OA-ICOS) experimental setup was built at a 2 μm band. On the basis of an off-axis integrated output spectroscopy (OA-ICOS), combined with an optical reinjection (RE) approach to improve signal intensity, and wavelength modulation spectroscopy (WMS) to improve the signal-to-noise ratio (SNR) of the system, the experimental study of trace CO2 with high sensitivity was carried out using the setup. The performance was compared and evaluated, and the results show that: Compared with the OA-ICOS, the wavelength modulated reinjection OA-ICOS enhanced the signal intensity by 6.3 times, and the SNR increased 7.2 times from 179 to 1288. The Allan variance results showed that the detection limit of the system is 0.35 ppm when the average system time is 230 s. The setup was used to measure the indoor CO2 concentration for a long time (22 h), and the measured results were in line with the actual concentration change. The proposed method shows good performance enhancement for the OA-ICOS system in trace gas measurements.
Forecasting of Drought: A Case Study of Water-Stressed Region of Pakistan
Authors: Kumar Prem , Shah Syed Feroz , Uqaili Mohammad Aslam , Kumar Laveet , Zafar Raja Fawad
Published: September 2021

Demand for water resources has increased dramatically due to the global increase in consumption of water, which has resulted in water depletion. Additionally, global climate change has further resulted as an impediment to human survival. Moreover, Pakistan is among the countries that have already crossed the water scarcity line, experiencing drought in the water-stressed Thar desert. Drought mitigation actions can be effectively achieved by forecasting techniques. This research describes the application of a linear stochastic model, i.e., Autoregressive Integrated Moving Average (ARIMA), to predict the drought pattern. The Standardized Precipitation Evapotranspiration Index (SPEI) is calculated to develop ARIMA models to forecast drought in a hyper-arid environment. In this study, drought forecast is demonstrated by results achieved from ARIMA models for various time periods. Result shows that the values of p, d, and q (non-seasonal model parameter) and P, D, and Q (seasonal model parameter) for the same SPEI period in the proposed models are analogous where “p” is the order of autoregressive lags, q is the order of moving average lags and d is the order of integration. Additionally, these parameters show the strong likeness for Moving Average (M.A) and Autoregressive (A.R) parameter values. From the various developed models for the Thar region, it has been concluded that the model (0,1,0)(1,0,2) is the best ARIMA model at 24 SPEI and could be considered as a generalized model. In the (0,1,0) model, the A.R term is 0, the difference/order of integration is 1 and the moving average is 0, and in the model (1,0,2) whose A.R has the 1st lag, the difference/order of integration is 0 and the moving average has 2 lags. Larger values for R2 greater than 0.9 and smaller values of Mean Error (ME), Mean Absolute Error (MAE), Mean Percentile Error (MPE), Mean Absolute Percentile Error (MAPE), and Mean Absolute Square Error (MASE) provide the acceptance of the generalized model. Consequently, this research suggests that drought forecasting can be effectively fulfilled by using ARIMA models, which can be assist policy planners of water resources to place safeguards keeping in view the future severity of the drought.
Drought Trends in the Polish Carpathian Mts. in the Years 1991–2020
Authors: Bokwa Anita , Klimek Mariusz , Krzaklewski Paweł , Kukułka Wojciech
Published: September 2021

Mountains are highly sensitive to the effects of climate change, including extreme short- and long-term weather phenomena. Therefore, in spite of relatively high annual precipitation totals, mountains might become endangered by droughts. The paper presents drought trends in the Polish Carpathians located in Central Europe. Data from the period 1991–2020 from 12 meteorological stations located in various vertical climate zones of the mountains were used to define drought conditions using the following indices: Standardized Precipitation (SPI), Standardized Precipitation Evapotranspiration (SPEI), Relative Precipitation (RPI) and Sielianinov. Additionally, four forest drought indices were used in order to estimate the impact of drought on beech as a typical Carpathian tree species, i.e., the Ellenberg (EQ), Forestry Aridity (FAI), Mayr Tetratherm (MT) and De Martonne Aridity (AI) indices. Statistically significant but weak trends were obtained for the 6-month SPI for four stations (indicating an increase in seasonal to mid-term precipitation), for the 1-month SPEI for three stations, for the 3-month SPEI for four stations, and for MT for all stations (indicating an increase in drought intensity). The analysis of dry month frequency according to particular indices shows that at most of the stations during the last decade of the study period, the frequency of dry months was much higher than in previous decades, especially in the cold half-year. Two zones of the Polish Carpathians are the most prone to drought occurrence: the peak zone due to the shift in climatic vertical zones triggered by the air temperature increase, and the forelands and foothills, together with basins located about 200–400 m a.s.l., where the mean annual air temperature is the highest in all the vertical profile, the annual sums of precipitation are very diversified, and the conditions for beech are already unfavorable.
Big Data Contribution in Desktop and Mobile Devices Comparison, Regarding Airlines’ Digital Brand Name Effect
Authors: Sakas Damianos P. , Giannakopoulos Nikolaos Th.
Published: September 2021

Rising demand for optimized digital marketing strategies has led firms in a hunt to harvest every possible aspect indicating users’ experience and preference. People visit, regularly through the day, numerous websites using both desktop and mobile devices. For businesses to acknowledge device’s usage rates is extremely important. Thus, this research is focused on analyzing each device’s usage and their effect on airline firms’ digital brand name. In the first phase of the research, we gathered web data from 10 airline firms during an observation period of 180 days. We then proceeded in developing an exploratory model using Fuzzy Cognitive Mapping, as well as a predictive and simulation model using Agent-Based Modeling. We inferred that various factors of airlines’ digital brand name are affected by both desktop and mobile usage, with mobile usage having a slightly bigger impact on most of them, with gradually rising values. Desktop device usage also appeared to be quite significant, especially in traffic coming from referral sources. The paper’s contribution has been to provide a handful of time-accurate insights for marketeers, regarding airlines’ digital marketing strategies.
Human Peripheral Nerve-Derived Pluripotent Cells Can Be Stimulated by In Vitro Bone Morphogenetic Protein-2
Authors: Sun Renyi , Jia Tanghong , Dart Bradley , Shrestha Sunaina , Bretches Morgan , Heggeness Michael H. , Yang Shang-You
Published: September 2021

We have recently identified a population of cells within the peripheral nerves of adult rodent animals (mice and rats) that can respond to Bone Morphogenetic Protein-2 (BMP-2) exposure or physical injury to rapidly proliferate. More importantly, these cells exhibited embryonic differentiation potentials that could be induced into osteoblastic and endothelial cells in vitro. The current study examined human nerve specimens to compare and characterize the cells after BMP-2 stimulation. Fresh pieces of human nerve tissue were minced and treated with either BMP-2 (750 ng/mL) or a PBS vehicle for 12 h at 37 °C, before being digested in 0.2% collagenase and 0.05% trypsin-EDTA. Isolated cells were cultured in a restrictive stem cell medium. Significantly more cells were obtained from the nerve pieces with the BMP-2 treatment in comparison with the PBS vehicle controls. Cell colonies started to form at Day 3. Expressions of the four transcription factors, namely, Klf4, c-Myc, Sox2, and Oct4, were confirmed at both the transcriptional and translational levels. The cells can be maintained in the stem cell culture medium for at least 6 weeks without changing their morphology. When the cells were transferred to a fibroblast growth medium, dispersed spindle-shaped motile cells were noted and became fibroblast activated protein-α (FAP) positive with immunocytochemistry staining. The data suggest that human peripheral nerve tissue also contains a population of cells that can respond to BMP-2 and express Klf4, Sox2, cMyc, and Oct4—the four transcription factors driving cell pluripotency. These cells are able to differentiate into FAP-positive fibroblasts. In summary, in human peripheral nerves also reside a population of quiescent cells with pluripotency potential that may be the same cells as rodent nerve-derived adult stem (NEDAPS) cells. It is proposed that these cells are possibly at the core of a previously unknown natural mechanism for healing an injury.
Reporting Guidelines for Whole-Body Vibration Studies in Humans, Animals and Cell Cultures: A Consensus Statement from an International Group of Experts
Authors: van Heuvelen Marieke J. G. , Rittweger Jörn , Judex Stefan , Sañudo Borja , Seixas Adérito , Fuermaier Anselm B. M. , Tucha Oliver , Nyakas Csaba , Marín Pedro J. , Taiar Redha , Stark Christina , Schoenau Eckhard , Sá-Caputo Danúbia C. , Bernardo-Filho Mario , van der Zee Eddy A.
Published: September 2021

Whole-body vibration (WBV) is an exercise modality or treatment/prophylaxis method in which subjects (humans, animals, or cells) are exposed to mechanical vibrations through a vibrating platform or device. The vibrations are defined by their direction, frequency, magnitude, duration, and the number of daily bouts. Subjects can be exposed while performing exercises, hold postures, sitting, or lying down. Worldwide, WBV has attracted significant attention, and the number of studies is rising. To interpret, compare, and aggregate studies, the correct, complete, and consistent reporting of WBV-specific data (WBV parameters) is critical. Specific reporting guidelines aid in accomplishing this goal. There was a need to expand existing guidelines because of continuous developments in the field of WBV research, including but not limited to new outcome measures regarding brain function and cognition, modified designs of WBV platforms and attachments (e.g., mounting a chair on a platform), and comparisons of animal and cell culture studies with human studies. Based on Delphi studies among experts and using EQUATOR recommendations, we have developed extended reporting guidelines with checklists for human and animal/cell culture research, including information on devices, vibrations, administration, general protocol, and subjects. In addition, we provide explanations and examples of how to report. These new reporting guidelines are specific to WBV variables and do not target research designs in general. Researchers are encouraged to use the new WBV guidelines in addition to general design-specific guidelines.
The Possible Role of Microbial Proteases in Facilitating SARS-CoV-2 Brain Invasion
Authors: Mjokane Nozethu , Folorunso Olufemi S. , Ogundeji Adepemi O. , Sebolai Olihile M.
Published: September 2021

SARS-CoV-2 has been shown to display proclivity towards organs bearing angiotensin-converting enzyme (ACE2) expression cells. Of interest herein is the ability of the virus to exhibit neurotropism. However, there is limited information on how this virus invades the brain. With this contribution, we explore how, in the context of a microbial co-infection using a cryptococcal co-infection as a model, SARS-CoV-2 could reach the brain. We theorise that the secretion of proteases by disseminated fungal cells might also activate the S2 domain of the viral spike glycoprotein for membrane fusion with brain endothelial cells leading to endocytosis. Understanding this potential invasion mechanism could lead to better SARS-CoV-2 intervention measures, which may also be applicable in instances of co-infection, especially with protease-secreting pathogens.
Probiotics and Trained Immunity
Authors: Cortes-Perez Naima G. , de Moreno de LeBlanc Alejandra , Gomez-Gutierrez Jorge G. , LeBlanc Jean Guy , Bermúdez-Humarán Luis G.
Published: September 2021

The characteristics of innate immunity have recently been investigated in depth in several research articles, and original findings suggest that innate immunity also has a memory capacity, which has been named “trained immunity”. This notion has revolutionized our knowledge of the innate immune response. Thus, stimulation of trained immunity represents a therapeutic alternative that is worth exploring. In this context, probiotics, live microorganisms which when administered in adequate amounts confer a health benefit on the host, represent attractive candidates for the stimulation of trained immunity; however, although numerous studies have documented the beneficial proprieties of these microorganisms, their mechanisms of action are not yet fully understood. In this review, we propose to explore the putative connection between probiotics and stimulation of trained immunity.
Caveolin-1 in Kidney Chronic Antibody-Mediated Rejection: An Integrated Immunohistochemical and Transcriptomic Analysis Based on the Banff Human Organ Transplant (B-HOT) Gene Panel
Authors: Gambella Alessandro , Barreca Antonella , Osella-Abate Simona , Bottasso Emanuel , Giarin Manuela Maria , Papotti Mauro , Biancone Luigi , Metovic Jasna , Collemi Giammarco , Cassoni Paola , Bertero Luca
Published: September 2021

Caveolin-1 overexpression has previously been reported as a marker of endothelial injury in kidney chronic antibody-mediated rejection (c-ABMR), but conclusive evidence supporting its use for daily diagnostic practice is missing. This study aims to evaluate if Caveolin-1 can be considered an immunohistochemical surrogate marker of c-ABMR. Caveolin-1 expression was analyzed in a selected series of 22 c-ABMR samples and 11 controls. Caveolin-1 immunohistochemistry proved positive in peritubular and glomerular capillaries of c-ABMR specimens, irrespective of C4d status whereas all controls were negative. Multiplex gene expression profiling in c-ABMR cases confirmed Caveolin-1 overexpression and identified additional genes (n = 220) and pathways, including MHC Class II antigen presentation and Type II interferon signaling. No differences in terms of gene expression (including Caveolin-1 gene) were observed according to C4d status. Conversely, immune cell signatures showed a NK-cell prevalence in C4d-negative samples compared with a B-cell predominance in C4d-positive cases, a finding confirmed by immunohistochemical assessment. Finally, differentially expressed genes were observed between c-ABMR and controls in pathways associated with Caveolin-1 functions (angiogenesis, cell metabolism and cell–ECM interaction). Based on our findings, Caveolin-1 resulted as a key player in c-ABMR, supporting its role as a marker of this condition irrespective of C4d status.
Bile Duct Ligation Upregulates Expression and Function of L-Amino Acid Transporter 1 at Blood–Brain Barrier of Rats via Activation of Aryl Hydrocarbon Receptor by Bilirubin
Authors: Zheng Xiaoke , Yang Hanyu , Qin Lan , Wang Siqian , Xie Lei , Yang Lu , Kong Weimin , Zhu Liang , Liu Li , Liu Xiaodong
Published: September 2021

Liver failure is associated with increased levels of brain aromatic amino acids (AAAs), whose transport across the blood–brain barrier (BBB) is mainly mediated by L-amino acid transporter 1 (LAT1). We aimed to investigate whether liver failure induced by bile duct ligation (BDL) increases levels of brain AAAs by affecting the expression and function of LAT1. The LAT1 function was assessed using the brain distribution of gabapentin. It was found that BDL significantly increased levels of gabapentin, phenylalanine, and tryptophan in the cortex, hippocampus, and striatum of rats, and upregulated the expression of total LAT1 protein in hippocampus and striatum as well as cortex membrane LAT1 protein. HCMEC/D3 served as in vitro BBB model, and the data showed that both the serum of BDL rats and bilirubin induced LAT1 expression and function, while bilirubin oxidase almost abolished the upregulation of LAT1 protein by bilirubin and the serum of BDL rats. The enhanced function and expression of LAT1 were also observed in the hippocampus and striatum of hyperbilirubinemia rats. Both aryl hydrocarbon receptor (AhR) antagonist α-naphthoflavone and AhR silencing obviously attenuated the upregulation of LAT1 protein by bilirubin or omeprazole. This study provides the first evidence that BDL upregulates LAT1 at the rat BBB, attributed to the activation of AhR by the increased plasma bilirubin. The results highlight the mechanisms causing BDL-increased levels of brain AAAs and their physiological significance.
Chronic Restraint Stress-Induced Muscle Atrophy Leads to Fatigue in Mice by Inhibiting the AMPK Signaling Pathway
Authors: Wang Zhi , Xia Tianji , Jin Suwei , Liu Xinmin , Pan Ruile , Yan Mingzhu , Chang Qi
Published: September 2021

Currently, an increasing number of people are suffering from fatigue due to the state of their lifestyles, such as sedentary work in a relatively small space, irregular sleep patterns, or the lack of movement and exercise. The present study was designed to simulate the occurrence of fatigue in the above populations through a chronic restraint stress (CRS) model, and to reveal its dynamic processes and potential underlying molecular mechanisms. ICR mice were subjected to 8 h of restraint stress each day for 5, 10, or 15 days. It was found that the weight-loaded swimming performance, grip strength, and locomotor activity of the mice all decreased under CRS treatment, and that up to 15 days of CRS induced notable fatigue. Gastrocnemius muscle atrophy and some abnormal biochemical parameters related to fatigue under CRS were observed. Furthermore, transcriptome data showed that the changes in muscle cell metabolism and mitochondrial dysfunction were associated with the AMPK signaling pathway in CRS-treated mice. Western blotting analysis of the AMPK/PGC-1α signaling pathway revealed that CRS could decrease mitochondrial biogenesis and reduce the numbers of type I skeletal muscle fibers in the gastrocnemius of mice. CRS could also block the protective mitophagic flux to inhibit the abnormal clearance of damaged mitochondria. Our study suggests a critical link between muscle atrophy and CRS-induced fatigue in mice, suggesting that the pharmacological promotion of muscle and mitochondrial function can be used as a treatment for stress-induced fatigue.
An Alternative Cell Therapy for Cancers: Induced Pluripotent Stem Cell (iPSC)-Derived Natural Killer Cells
Authors: Hsu Li-Jie , Liu Chao-Lin , Kuo Ming-Ling , Shen Chia-Ning , Shen Chia-Rui
Published: September 2021

Cell therapy is usually defined as the treatment or prevention of human disease by supplementation with cells that have been selected, manipulated, and pharmacologically treated or altered outside the body (ex vivo). Induced pluripotent stem cells (iPSCs), with their unique characteristics of indefinite expansion in cultures and genetic modifications, represent an ideal cell source for differentiation into specialized cell types. Cell therapy has recently become one of the most promising therapeutic approaches for cancers, and different immune cell types are selected as therapeutic platforms. Natural killer (NK) cells are shown to be effective tumor cell killers and do not cause graft-vs-host disease (GVHD), making them excellent candidates for, and facilitating the development of, “off-the-shelf” cell therapies. In this review, we summarize the progress in the past decade in the advent of iPSC technology and review recent developments in gene-modified iPSC-NK cells as readily available “off-the-shelf” cellular therapies.
Mambalgin-2 Inhibits Growth, Migration, and Invasion of Metastatic Melanoma Cells by Targeting the Channels Containing an ASIC1a Subunit Whose Up-Regulation Correlates with Poor Survival Prognosis
Authors: Bychkov Maxim L. , Kirichenko Artem V. , Shulepko Mikhail A. , Mikhaylova Irina N. , Kirpichnikov Mikhail P. , Lyukmanova Ekaterina N.
Published: September 2021

Melanoma is an aggressive cancer characterized by the acidification of the extracellular environment. Here, we showed for the first time that extracellular media acidification increases proliferation, migration, and invasion of patient-derived metastatic melanoma cells and up-regulates cell-surface expression of acid-sensitive channels containing the ASIC1a, α-ENaC, and γ-ENaC subunits. No influence of media acidification on these processes was found in normal keratinocytes. To control metastatic melanoma progression associated with the ASIC1a up-regulation, we proposed the ASIC1a inhibitor, -mambalgin-2 from Dendpoaspis polylepis venom. Recombinant analog of mambalgin-2 cancelled acidification-induced proliferation, migration, and invasion of metastatic melanoma cells, promoted apoptosis, and down-regulated cell-surface expression of prooncogenic factors CD44 and Frizzled 4 and phosphorylation of transcription factor SNAI. Confocal microscopy and affinity purification revealed that mambalgin-2 interacts with heterotrimeric ASIC1a/α-ENaC/γ-ENaC channels on the surface of metastatic melanoma cells. Using the mutant variant of mambalgin-2 with reduced activity toward ASIC1a, we confirmed that the principal molecular target of mambalgin-2 in melanoma cells is the ASIC1a subunit. Bioinformatic analysis confirmed up-regulation of the ASIC1 expression as a marker of poor survival prognosis for patients with metastatic melanoma. Thus, targeting ASIC1a by drugs such as mambalgin-2 could be a promising strategy for metastatic melanoma treatment.
WISP-3 Stimulates VEGF-C-Dependent Lymphangiogenesis in Human Chondrosarcoma Cells by Inhibiting miR-196a-3p Synthesis
Authors: Lin Chih-Yang , Wang Shih-Wei , Guo Jeng-Hung , Tai Huai-Ching , Sun Wen-Chun , Lai Cheng-Ta , Yang Chen-Yu , Liu Shih-Chia , Fong Yi-Chin , Tang Chih-Hsin
Published: September 2021

Chondrosarcoma is a malignant bone tumor with high metastatic potential. Lymphangiogenesis is a critical biological step in cancer metastasis. WNT1-inducible signaling pathway protein 3 (WISP-3) regulates angiogenesis and facilitates chondrosarcoma metastasis, but the role of WISP-3 in chondrosarcoma lymphangiogenesis is unclear. In this study, incubation of chondrosarcoma cells with WISP-3 increased the production of VEGF-C, an important lymphangiogenic factor. Conditioned medium from WISP-3-treated chondrosarcoma cells significantly enhanced lymphatic endothelial cell tube formation. WISP-3-induced stimulation of VEGF-C-dependent lymphangiogenesis inhibited miR-196a-3p synthesis in the ERK, JNK, and p38 signaling pathways. This evidence suggests that the WISP-3/VEGF-C axis is worth targeting in the treatment of lymphangiogenesis in human chondrosarcoma.
New Aerodynamic Studies of an Adaptive Winglet Application on the Regional Jet CRJ700
Authors: Segui Marine , Abel Federico R. , Botez Ruxandra M. , Ceruti Alessandro
Published: September 2021

This study aims to evaluates how an adaptive winglet during flight can improve aircraft aerodynamic characteristics of the CRJ700. The aircraft geometry was slightly modified to integrate a one-rotation axis adaptive winglet. Aerodynamic characteristics of the new adaptive design were computed using a validated high-fidelity aerodynamic model developed with the open-source code OpenFoam. The aerodynamic model successively uses the two solvers simpleFoam and rhoSimpleFoam based on Reynold Averaged Navier Stokes equations. Characteristics of the adaptive winglet design were studied for 16 flight conditions, representative of climb and cruise usually considered by the CRJ700. The adaptive winglet can increase the lift-to-drag ratio by up to 6.10% and reduce the drag coefficient by up to 2.65%. This study also compared the aerodynamic polar and pitching moment coefficients variations of the Bombardier CRJ700 equipped with an adaptive versus a fixed winglet.
Neurophysiological Factors Affecting Muscle Innervation Zone Estimation Using Surface EMG: A Simulation Study
Authors: Huang Chengjun , Chen Maoqi , Li Xiaoyan , Zhang Yingchun , Li Sheng , Zhou Ping
Published: September 2021

Surface electromyography (EMG) recorded by a linear or 2-dimensional electrode array can be used to estimate the location of muscle innervation zones (IZ). There are various neurophysiological factors that may influence surface EMG and thus potentially compromise muscle IZ estimation. The objective of this study was to evaluate how surface-EMG-based IZ estimation might be affected by different factors, including varying degrees of motor unit (MU) synchronization in the case of single or double IZs. The study was performed by implementing a model simulating surface EMG activity. Three different MU synchronization conditions were simulated, namely no synchronization, medium level synchronization, and complete synchronization analog to M wave. Surface EMG signals recorded by a 2-dimensional electrode array were simulated from a muscle with single and double IZs, respectively. For each situation, the IZ was estimated from surface EMG and compared with the one used in the model for performance evaluation. For the muscle with only one IZ, the estimated IZ location from surface EMG was consistent with the one used in the model for all the three MU synchronization conditions. For the muscle with double IZs, at least one IZ was appropriately estimated from interference surface EMG when there was no MU synchronization. However, the estimated IZ was different from either of the two IZ locations used in the model for the other two MU synchronization conditions. For muscles with a single IZ, MU synchronization has little effect on IZ estimation from electrode array surface EMG. However, caution is required for multiple IZ muscles since MU synchronization might lead to false IZ estimation.
What Is Social about Autism? The Role of Allostasis-Driven Learning
Authors: Djerassi Meshi , Ophir Shachar , Atzil Shir
Published: September 2021

Scientific research on neuro-cognitive mechanisms of autism often focuses on circuits that support social functioning. However, autism is a heterogeneous developmental variation in multiple domains, including social communication, but also language, cognition, and sensory-motor control. This suggests that the underlying mechanisms of autism share a domain-general foundation that impacts all of these processes. In this Perspective Review, we propose that autism is not a social deficit that results from an atypical “social brain”. Instead, typical social development relies on learning. In social animals, infants depend on their caregivers for survival, which makes social information vitally salient. The infant must learn to socially interact in order to survive and develop, and the most prominent learning in early life is crafted by social interactions. Therefore, the most prominent outcome of a learning variation is atypical social development. To support the hypothesis that autism results from a variation in learning, we first review evidence from neuroscience and developmental science, demonstrating that typical social development depends on two domain-general processes that determine learning: (a) motivation, guided by allostatic regulation of the internal milieu; and (b) multi-modal associations, determined by the statistical regularities of the external milieu. These two processes are basic ingredients of typical development because they determine allostasis-driven learning of the social environment. We then review evidence showing that allostasis and learning are affected among individuals with autism, both neurally and behaviorally. We conclude by proposing a novel domain-general framework that emphasizes allostasis-driven learning as a key process underlying autism. Guided by allostasis, humans learn to become social, therefore, the atypical social profile seen in autism can reflect a domain-general variation in allostasis-driven learning. This domain-general view raises novel research questions in both basic and clinical research and points to targets for clinical intervention that can lower the age of diagnosis and improve the well-being of individuals with autism.
A Review of Methods for Sleep Arousal Detection Using Polysomnographic Signals
Authors: Qian Xiangyu , Qiu Ye , He Qingzu , Lu Yuer , Lin Hai , Xu Fei , Zhu Fangfang , Liu Zhilong , Li Xiang , Cao Yuping , Shuai Jianwei
Published: September 2021

Multiple types of sleep arousal account for a large proportion of the causes of sleep disorders. The detection of sleep arousals is very important for diagnosing sleep disorders and reducing the risk of further complications including heart disease and cognitive impairment. Sleep arousal scoring is manually completed by sleep experts by checking the recordings of several periods of sleep polysomnography (PSG), which is a time-consuming and tedious work. Therefore, the development of efficient, fast, and reliable automatic sleep arousal detection system from PSG may provide powerful help for clinicians. This paper reviews the automatic arousal detection methods in recent years, which are based on statistical rules and deep learning methods. For statistical detection methods, three important processes are typically involved, including preprocessing, feature extraction and classifier selection. For deep learning methods, different models are discussed by now, including convolution neural network (CNN), recurrent neural network (RNN), long-term and short-term memory neural network (LSTM), residual neural network (ResNet), and the combinations of these neural networks. The prediction results of these neural network models are close to the judgments of human experts, and these methods have shown robust generalization capabilities on different data sets. Therefore, we conclude that the deep neural network will be the main research method of automatic arousal detection in the future.
Effects of tDCS on Language Recovery in Post-Stroke Aphasia: A Pilot Study Investigating Clinical Parameters and White Matter Change with Diffusion Imaging
Authors: Soliman Radwa K. , Tax Chantal M. W. , Abo-Elfetoh Noha , Karim Ahmed A. , Youssef Ayda , Kamal Doaa , Khedr Eman M.
Published: September 2021

Objectives: In this pilot study we investigated the effects of transcranial direct current stimulation (tDCS) on language recovery in the subacute stage of post-stroke aphasia using clinical parameters and diffusion imaging with constrained spherical deconvolution-based tractography. Methods: The study included 21 patients with subacute post-stroke aphasia. Patients were randomly classified into two groups with a ratio of 2:1 to receive real tDCS or sham tDCS as placebo control. Patients received 10 sessions (5/week) bi-hemispheric tDCS treatments over the left affected Broca’s area (anodal electrode) and over the right unaffected Broca’s area (cathodal stimulation). Aphasia score was assessed clinically using the language section of the Hemispheric Stroke Scale (HSS) before and after treatment sessions. Diffusion imaging and tractography were performed for seven patients of the real group, both before and after the 10th session. Dissection of language-related white matter tracts was achieved, and diffusion measures were extracted. A paired Student’s t-test was used to compare the clinical recovery and diffusion measures of the dissected tracts both pre- and post- treatment. The partial correlation between changes in diffusion measures and the language improvements was calculated. Results: At baseline assessment, there were no significant differences between groups in demographic and clinical HSS language score. No significant clinical recovery in HSS was evident in the sham group. However, significant improvements in the different components of HSS were only observed in patients receiving real tDCS. Associated significant increase in the fractional anisotropy of the right uncinate fasciculus and a significant reduction in the mean diffusivity of the right frontal aslant tract were reported. A significant positive correlation was found between the changes in the right uncinate fasciculus and fluency improvement. Conclusions: Aphasia recovery after bi-hemispheric transcranial direct current stimulation was associated with contralesional right-sided white matter changes at the subacute stage. These changes probably reflect neuroplasticity that could contribute to the recovery. Both the right uncinate fasciculus and right frontal aslant tract seem to be involved in aphasia recovery.
Meaning-Making Coping Methods among Bereaved Parents: A Pilot Survey Study in Sweden
Authors: Ahmadi Fereshteh , Zandi Saeid
Published: September 2021

The death of a child may result in traumatizing forms of grief, and meaning-making coping with loss seems to be important in prevention of intense psychosocial problems among bereaved parents. The aim of this quantitative pilot study was to discover the divergent meaning-making coping methods used by bereaved parents in Sweden. In doing so, 162 respondents were selected using a convenience sampling method, and they responded to the modified version of RCOPE. The study revealed that the strategies talking to others about their feelings, pondering the meaning of life alone, and being in nature for greater emotional affiliation, i.e., what we call secular existential coping methods, have been the most used meaning-making coping methods among Swedish mourning parents. While explaining the results, we considered the respondents’ cultural background and speculated about the potential influence of cultural teachings and elements in the selection of ways of coping with bereavement. Further, we compared the results obtained with those of the two other Swedish studies conducted among people coping with cancer and COVID-19 to further discuss the impact of culture on coping with illness, loss, grief, and crisis. The study supports the idea that culture plays an essential role in the choice of coping methods.
Promoting Health and Safety in Construction through the Procurement Process
Authors: Boadu Elijah Frimpong , Sunindijo Riza Yosia , Wang Cynthia Changxin
Published: September 2021

This study explored the impact of considering health and safety (H&S) in the construction procurement process based on the extent of H&S implementation on projects. Underpinned by information integration and rational decision-making theories, the study evaluated how the integration of H&S objectives into the overall project objectives, and the subsequent consideration of H&S matters in procurement decisions, influence H&S implementation on projects. Data were collected using questionnaire surveys from 287 respondents in Ghana who had direct involvement in the project procurement process. The survey explored the extent of H&S integration into the procurement process and its subsequent impact on H&S implementation. Path analysis was carried out to determine the causal relationships between the various procurement processes and H&S implementation. The results demonstrate that setting H&S objectives and integrating H&S into the planning stage decisions have a significant impact on the extent to which H&S matters are considered in the tendering and tender evaluation stages, as well as the H&S provisions in conditions of contracts. It also showed that adequate H&S consideration in these procurement stages subsequently influences H&S consideration in contract administration and monitoring and ultimately influences the extent of H&S implementation. These findings demonstrate the importance of integrating H&S in all aspects of construction procurement to promote H&S implementation on projects.
Real World Evidence of CAR T-Cell Therapies for the Treatment of Relapsed/Refractory B-Cell Non-Hodgkin Lymphoma: A Monocentric Experience
Authors: Casadei Beatrice , Argnani Lisa , Guadagnuolo Serafina , Pellegrini Cinzia , Stefoni Vittorio , Broccoli Alessandro , Nanni Laura , Morigi Alice , Lolli Ginevra , Guarino Maria , Spinardi Luca , Pierucci Elisabetta , Fanti Stefano , Bartoletti Michele , Dicataldo Michele , Sabattini Elena , Bonifazi Francesca , Zinzani Pier Luigi
Published: September 2021

Large B-cell lymphomas (LBCL) are the most common types of non-Hodgkin lymphoma. Although outcomes have improved thanks to the introduction of rituximab-based chemoimmunotherapy, certain LBCL still represents a challenge because of initial resistance to therapy or recurrent relapses. Axicabtagene ciloleucel (axi-cel) and tisagenlecleucel (tisa-cel) are second-generation autologous CD19-targeted chimeric antigen receptor (CAR) T-cell therapies approved for patients with relapsed/refractory (R/R) LBCL, based on the results of phase II pivotal single-arm trials ZUMA-1 (for axi-cel) and JULIET (for tisa-cel). Here, we report patients outcomes with axi-cel and tisa-cel in the standard of care (SoC) setting for R/R LBCL, treated at our Institution. Data were collected from patients who underwent leukapheresis between August 2019 and February 2021. Toxicities were graded and managed according to the institution’s guidelines. Responses were assessed as per Lugano 2014 classification. Of the 30 patients who underwent leukapheresis, 18 (60%) received axi-cel, while 12 (40%) tisa-cel. Grade 3 or higher cytokine release syndrome and neurotoxicity occurred in 10% and 16% patients, respectively. Best objective and complete response rates were 73.3% and 40%, respectively. Treatment in SoC setting with CD19 CAR T-cell therapies for R/R LBCL showed a manageable safety profile and high objective response rate.
Clinical Outcomes Following Re-Operations for Intracranial Meningioma
Authors: Richardson George E. , Gillespie Conor S. , Mustafa Mohammad A. , Taweel Basel A. , Bakhsh Ali , Kumar Siddhant , Keshwara Sumirat M. , Ali Tamara , John Bethan , Brodbelt Andrew R. , Chavredakis Emmanuel , Mills Samantha J. , May Chloë , Millward Christopher P. , Islim Abdurrahman I. , Jenkinson Michael D.
Published: September 2021

The outcomes following re-operation for meningioma are poorly described. The aim of this study was to identify risk factors for a performance status outcome following a second operation for a recurrent meningioma. A retrospective, comparative cohort study was conducted. The primary outcome measure was World Health Organization performance. Secondary outcomes were complications, and overall and progression free survival (OS and PFS respectively). Baseline clinical characteristics, tumor details, and operation details were collected. Multivariable binary logistic regression was used to identify risk factors for performance status outcome following a second operation. Between 1988 and 2018, 712 patients had surgery for intracranial meningiomas, 56 (7.9%) of which underwent a second operation for recurrence. Fifteen patients (26.8%) had worsened performance status after the second operation compared to three (5.4%) after the primary procedure (p = 0.002). An increased number of post-operative complications following the second operation was associated with a poorer performance status following that procedure (odds ratio 2.2 [95% CI 1.1–4.6]). The second operation complication rates were higher than after the first surgery (46.4%, n = 26 versus 32.1%, n = 18, p = 0.069). The median OS was 312.0 months (95% CI 257.8–366.2). The median PFS following the first operation was 35.0 months (95% CI 28.9–41.1). Following the second operation, the median PFS was 68.0 months (95% CI 49.1–86.9). The patients undergoing a second operation for meningioma had higher rates of post-operative complications, which is associated with poorer clinical outcomes. The decisions surrounding second operations must be balanced against the surgical risks and should take patient goals into consideration.
Thymic Lymphoepithelial Carcinoma Associated with Epstein-Barr Virus: Experiences and Literature Review
Authors: Ose Naoko , Kawagishi Sachi , Funaki Soichiro , Kanou Takashi , Fukui Eriko , Kimura Kenji , Minami Masato , Shintani Yasushi
Published: September 2021

Thymic lymphoepithelial carcinoma (TLEC) is a primary thymic carcinoma that accounts for about 14% of all thymic epithelial tumors and is classified into 14 types. The histological morphology is similar to lymphoepithelioma, a type of undifferentiated nasopharyngeal carcinoma. It has been reported that squamous carcinoma accounts for approximately 80% of thymic carcinoma, followed by TLEC, which accounts for 6%. TLEC has been reported to be associated with Epstein-Barr virus (EBV), with EBV infection in TLEC tumor cells first noted by Lyvraz et al. in 1985. Tumors shown to be EBV-positive are classified as TLEC if lymphoplasmacytic infiltration is lacking. However, only about 50% of the cases are positive for EBV, which is lower compared to nasopharyngeal lymphoepithelioma. Instances of EBV infection in other types of thymic epithelial tumor have been reported at lower rates, which suggests that EBV infection may have an important influence on the carcinogenesis of TLEC, though the etiology is unknown. TLEC is a highly malignant tumor with poor prognosis, as affected patients have a median survival time of 22 months, according to 58 cases from the literature, while the 5-year survival rate is 34.4%. Presently, prognosis is not considered to be affected by the presence or absence of EBV positivity.
Sulforaphane: A Broccoli Bioactive Phytocompound with Cancer Preventive Potential
Authors: Kaiser Anna E. , Baniasadi Mojdeh , Giansiracusa Derrek , Giansiracusa Matthew , Garcia Michael , Fryda Zachary , Wong Tin Lok , Bishayee Anupam
Published: September 2021

There is substantial and promising evidence on the health benefits of consuming broccoli and other cruciferous vegetables. The most important compound in broccoli, glucoraphanin, is metabolized to SFN by the thioglucosidase enzyme myrosinase. SFN is the major mediator of the health benefits that have been recognized for broccoli consumption. SFN represents a phytochemical of high interest as it may be useful in preventing the occurrence and/or mitigating the progression of cancer. Although several prior publications provide an excellent overview of the effect of SFN in cancer, these reports represent narrative reviews that focused mainly on SFN’s source, biosynthesis, and mechanisms of action in modulating specific pathways involved in cancer without a comprehensive review of SFN’s role or value for prevention of various human malignancies. This review evaluates the most recent state of knowledge concerning SFN’s efficacy in preventing or reversing a variety of neoplasms. In this work, we have analyzed published reports based on in vitro, in vivo, and clinical studies to determine SFN’s potential as a chemopreventive agent. Furthermore, we have discussed the current limitations and challenges associated with SFN research and suggested future research directions before broccoli-derived products, especially SFN, can be used for human cancer prevention and intervention.