bluuebunny/crossref_metadata_2025 huggingface dataset:
Is acupoint injection the optimal way to administer mecobalamin for diabetic peripheral neuropathy? A meta-analysis and trial sequential analysis
Authors: Zhang Fei , Yu Yunfeng , Yin Shuang , Hu Gang , Yang Xinyu , Tong Keke , Yu Rong
Published: October 2023

Objective

Mecobalamin is a commonly used drug in the treatment of diabetic peripheral neuropathy (DPN). This study aimed to systematically evaluate the efficacy and safety of acupoint injection of mecobalamin for DPN.

Methods

Relevant clinical trials on acupoint injection of mecobalamin for DPN published before 31 January 2023 were searched in eight commonly used databases. After screening and confirming the included studies, meta-analysis and trial sequential analysis were performed.

Results

A total of 10 relevant studies were confirmed, and the total sample size was 927 cases. On the efficacy endpoints, meta-analysis showed that compared with other administration methods, acupoint injection of mecobalamin significantly increased the clinical effective rate by 27% [RR = 1.27, 95% CI = (1.19, 1.36), P < 0.00001], motor nerve conduction velocity (median nerve) by 5.93 m/s [MD = 5.93, 95% CI = (4.79, 7.07), P < 0.00001], motor nerve conduction velocity (common peroneal nerve) by 5.66 m/s [MD = 5.66, 95% CI = (2.89, 8.43), P < 0.0001], sensory nerve conduction velocity (median nerve) by 4.83 m/s [MD = 4.83, 95% CI = (3.75, 5.90), P < 0.00001], and sensory nerve conduction velocity (common peroneal nerve) by 3.60 m/s [MD = 3.60, 95% CI = (2.49, 4.71), P < 0.00001], and trial sequential analysis showed these benefits were conclusive. In terms of safety endpoints, meta-analysis indicated that the total adverse events for acupoint injection were comparable to other methods of administration, and trial sequential analysis suggested that the results needed to be validated by more studies. Subgroup analysis demonstrated that the benefits of acupoint injections of mecobalamin were not limited by the dose, duration of treatment, or number of acupoints reported in the included studies. Harbord's test showed no significant publication bias (P = 0.106).

Conclusion

The efficacy of acupoint injection of mecobalamin for DPN was significantly better than other administrations, and its safety was comparable to other administrations. Therefore, acupoint injection may be the optimal method of mecobalamin for DPN.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=454120, identifier: CRD42023454120.
Transcutaneous auricular vagus nerve stimulation in the treatment of disorders of consciousness: mechanisms and applications
Authors: Wang Likai , Gao Fei , Wang Zhan , Liang Feng , Dai Yongli , Wang Mengchun , Wu Jingyi , Chen Yaning , Yan Qinjie , Wang Litong
Published: October 2023

This review provides an in-depth exploration of the mechanisms and applications of transcutaneous auricular vagus nerve stimulation (taVNS) in treating disorders of consciousness (DOC). Beginning with an exploration of the vagus nerve’s role in modulating brain function and consciousness, we then delve into the neuroprotective potential of taVNS demonstrated in animal models. The subsequent sections assess the therapeutic impact of taVNS on human DOC, discussing the safety, tolerability, and various factors influencing the treatment response. Finally, the review identifies the current challenges in taVNS research and outlines future directions, emphasizing the need for large-scale trials, optimization of treatment parameters, and comprehensive investigation of taVNS’s long-term effects and underlying mechanisms. This comprehensive overview positions taVNS as a promising and safe modality for DOC treatment, with a focus on understanding its intricate neurophysiological influence and optimizing its application in clinical settings.
Plant protein consumption is associated with body mass index among women of reproductive age in Indonesia
Authors: Sistia Fitra , Khusun Helda , Februhartanty Judhiastuty
Published: October 2023

Introduction

One of the known determinants of obesity in Southeast Asia countries, including Indonesia, is the nutritional transition, which is indicated by fast changes in food production, dietary habits, and physical activity. With rising incomes, plant protein from grains, tubers, and legumes is gradually being replaced by animal protein from poultry, eggs, dairy, and red meat. This change is identified as a protein transition. Different choices of protein sources in the diet have varying health effects. However, there is limited information on the Asian population on the role of protein consumption on the increasing obesity prevalence. Therefore, this study aimed to investigate the association of protein sources consumption with body mass index (BMI) among women of reproductive age in Indonesia.

Methods

This study used secondary data from the 2018 Indonesia Food Barometer (IFB) conducted using a quantitative cross-sectional survey. A total of 467 Indonesian reproductive-aged women (20–49 years) were included in this study. Dietary intake, including protein consumption, was obtained using 24-h dietary recall. Multiple linear regression was applied to find the association of protein consumption with BMI with a p-value <0.05 considered as a significant outcome variable.

Results

The Mean BMI was 25.02 kg/m2, median of animal and plant protein was 28.01 g/day and 25.37 g/day, respectively. Consumption of plant protein was significantly associated with BMI after adjusting for marital status and age (p-value = 0.043; R2 = 0.080). The quality of plant protein should be considered to prevent obesity problems among women of reproductive age.
Discovery of a haptoglobin glycopeptides biomarker panel for early diagnosis of hepatocellular carcinoma
Authors: Kohansal-Nodehi Mahdokht , Swiatek-de Lange Magdalena , Kroeniger Konstantin , Rolny Vinzent , Tabarés Glòria , Piratvisuth Teerha , Tanwandee Tawesak , Thongsawat Satawat , Sukeepaisarnjaroen Wattana , Esteban Juan Ignacio , Bes Marta , Köhler Bruno , Chan Henry Lik-Yuen , Busskamp Holger
Published: October 2023

Background

There is a need for new serum biomarkers for early detection of hepatocellular carcinoma (HCC). Haptoglobin (Hp) N-glycosylation is altered in HCC, but the diagnostic value of site-specific Hp glycobiomarkers is rarely reported. We aimed to determine the site-specific glycosylation profile of Hp for early-stage HCC diagnosis.

Method

Hp glycosylation was analyzed in the plasma of patients with liver diseases (n=57; controls), early-stage HCC (n=50) and late-stage HCC (n=32). Hp phenotype was determined by immunoblotting. Hp was immunoisolated and digested into peptides. N-glycopeptides were identified and quantified using liquid chromatography–mass spectrometry. Cohort samples were compared using Wilcoxon rank-sum (Mann-Whitney U) tests. Diagnostic performance was assessed using receiver operating characteristic (ROC) curves and area under curve (AUC).

Results

Significantly higher fucosylation, branching and sialylation of Hp glycans, and expression of high-mannose glycans, was observed as disease progressed from cirrhosis to early- and late-stage HCC. Several glycopeptides demonstrated high values for early diagnosis of HCC, with an AUC of 93% (n=1), >80% (n=3), >75% (n=13) and >70% (n=11), compared with alpha-fetoprotein (AFP; AUC of 79%). The diagnostic performance of the identified biomarkers was only slightly affected by Hp phenotype.

Conclusion

We identified a panel of Hp glycopeptides that are significantly differentially regulated in early- and late-stage HCC. Some glycobiomarkers exceeded the diagnostic value of AFP (the most commonly used biomarker for HCC diagnosis). Our findings provide evidence that glycobiomarkers can be effective in the diagnosis of early HCC – individually, as a panel of glycopeptides or combined with conventional serological biomarkers.
The journey of stage III and IV non-small cell lung cancer patients in the Brazilian private healthcare system: a retrospective study
Authors: Cerqueira Erica R. , Batista Paula M. , Almeida Milena F. , Rego Maria A. C. , Ribeiro-Pereira Ana C. P. , Alencar Fernando , Fernandes Roberta A. , Calabrich Aknar F. C. , Schvartsman Gustavo
Published: October 2023

Non-small cell lung cancer (NSCLC) is still diagnosed at late stages in Brazil. The availability of newer treatment options has changed patient management, however, few real-world data have been published since then. This is a population-based retrospective cohort study that aims to evaluate the characteristics of stage III/IV NSCLC patients and their journey in the Brazilian private healthcare system. Patients aged ≥18 years, residing in Brazil who had their first medical appointment between 2016 and 2018 were included in the study. The sociodemographic and clinical characteristics of the patients and time intervals of interest were described. A total of 10,394 patients were analyzed. The majority of the patients were male (58.5%) with a median age of 64.0 (IQR = 58.0 – 71.0) years. In relation to characteristics of the disease, most of the tumors were characterized as adenocarcinomas (52.3%) and diagnosed at stage IV (72.2%). Most patients arrived at the hospital with an established NSCLC diagnosis, while 45.7% were diagnosed at the first medical appointment in the hospital or later. For patients who were diagnosed at the first medical appointment or later, a median interval of 15.0 (IQR = 6.0 – 33.0) days was observed between the first medical appointment and the diagnosis. The first treatment was given after a median of 25.0 (IQR = 6.0 – 49.0) days after diagnosis for patients without a prior diagnosis, and 57.0 (IQR: 33.0 – 98.0) days for patients with a prior diagnosis. The most common treatments were chemotherapy alone (33.8%), chemotherapy combined with radiotherapy (21.5%), radiotherapy alone (13.1%), adjuvant or neoadjuvant treatment (9.3%), surgery (3.3%), and immunotherapy (0.7%; alone or combined). At the end of follow-up (September, 2020), 52.3% of the patients had died. Despite having more treatment options in the private sector, data show that there is a need to improve access to technologies.
Carcinosarcoma of the ovary: a case report and literature review
Authors: Zheng Jian , Tang Cui , Liu Ping , Hao Hua
Published: October 2023

Objective

Carcinosarcoma of the ovary is a rare pathological type of ovarian cancer that is highly aggressive and occurs most frequently in the female reproductive tract at the site of the uterus. Herein, we explore the clinicopathological features, diagnosis, differential diagnosis, and treatment options for carcinosarcoma of the ovary.

Methods

We analyzed the clinical data of a case of carcinosarcoma, observed its histological morphology and immunohistochemical characteristics, detected the homologous recombination repair deficiency gene mutation, and reviewed the relevant literature.

Results

A 76-year-old menopausal woman visited our hospital because of abdominal distension, difficulty in urination, and constipation. Ultrasonography demonstrated abnormalities in the uterus and pelvic cavity, suggesting that the patient should undergo surgery. Immunohistochemical findings of carcinosarcoma of the right ovary were as follows: CK fraction (+), vimentin fraction (+), CK5/6 foci (+), p16 (+), p53 in approximately 70% (+), WT-1 foci (+), ER foci (+), PR part (+), Her-2 (1+), CK7 fraction (+), CK20 foci (+), CD99 fraction (+), CD10 fraction (+), CD56 foci (+), c-kit foci (+), SMA part (+), desmin foci (+), PD-L1 (-), SALL4 (-), OCT3/4 (-), p63 (-), p40 (-), D2-40 (-), inhibin (-), PLAP (-), CD30 (-), and Ki67 hotspot in approximately 80% (+). The patient underwent tumor cytoreduction and adjuvant chemotherapy. Currently, she is being followed up for 16 months and has a good general condition.

Conclusion

The diagnosis of carcinosarcoma relies on histopathological examination and differentiation of carcinosarcoma from immature teratoma. The current therapeutic regimen for carcinosarcoma is still based on tumor cytoreduction and platinum-containing chemotherapy; research on targeted therapy is still in progress.
Prognostic significance of molecular subgroups in survival outcome for children with medulloblastoma in Malaysia
Authors: Rajagopal Revathi , Teng Ay Jiuan , Jawin Vida , Wong Oy Leng , Mahsin Hakimah , Abd Rani Nor Haizura , Yap Tsiao Yi , Gunasagaran Kogilavani , Thevarajah Asohan , Yeoh Seoh Leng , Ong Gek Bee , Ariffin Hany , Jones David , Bouffet Eric , Gottardo Nicholas G.
Published: October 2023

Introduction

Advancements in genomic profiling led to the discovery of four major molecular subgroups in medulloblastoma (MB), which have now been incorporated into the World Health Organization classification of central nervous system tumors. The current study aimed to determine the prognostic significance of the MB molecular subgroups among children in Malaysia.

Methods

We assembled MB samples from children <18 years between January 2003 and June 2017 from four pediatric oncology centers in Malaysia. MB was sub-grouped using 850k DNA methylation testing at German Cancer Research Centre, Heidelberg, Germany.

Results

Fifty samples from patients diagnosed and treated as MB were identified. Two (4%) of the 50 patients’ tumor DNA samples were insufficient for analysis. Of the remaining 48 patients, 41 (85%) samples were confirmed as MB, while for 7 (15%) patients, DNA methylation classification results were discrepant with the histopathological diagnosis of MB, with various other diagnoses. Of the 41 MB patients, 15 patients were stratified as standard-risk (SR), 16 patients as high-risk (HR), and ten as infants (age <3 years old). Molecular subgrouping of the whole cohort revealed four (14%) WNT, 11 (27%) SHH, 10 (24%) Group 3, and 16 (39%) Group 4. Treatment abandonment rates for older children and infants were 22.5% and 10%, respectively. After censoring treatment abandonment, for SR patients, the 5-year event-free survival (EFS) and overall survival (OS) were 43.1% ± 14.7% and 46.9 ± 15.6%, respectively, while in HR, 5-year EFS and OS were both 63.6% ± 14.5%. Infants had a 5-year EFS and OS of 55.6% ± 16.6% and 66.7% ± 15.7%, respectively. WNT tumors had the best 5y-OS, followed by Group 3, Group 4, and SHH in children ≥3 years old. In younger children, SHH MB patients showed favorable outcomes.

Conclusion

The study highlights the importance of DNA methylation profiling for diagnostic accuracy. Most infants had SHH MB, and their EFS and OS were comparable to those reported in high-income countries. Due to the relatively small cohort and the high treatment abandonment rate, definite conclusions cannot be made regarding the prognostic significance of molecular subgroups of MB. Implementing this high-technology investigation would assist pathologists in improving the diagnosis and provide molecular subgrouping of MB, permitting subgroup-specific therapies.
Review on neuroimaging in pediatric-type diffuse low-grade gliomas
Authors: Chen Jing , Qi Xin , Zhang Mengjie , Zhang Jing , Han Tong , Wang Chunxiang , Cai Chunquan
Published: October 2023

The fifth edition of the World Health Organization Classification of Tumors of the Central Nervous System (WHO CNS5) has identified a new classification system for tumors of the brain and spinal cord, highlighting the pivotal role of molecular diagnosis in accurately categorizing neoplasms. In addition to previous classifications, one of the key distinctions lies in categorizing pediatric-type diffuse low-grade gliomas (pDLGGs) and pediatric-type diffuse high-grade gliomas (pDHGGs) as distinct tumor types. Although similar in histology and morphology, pediatric diffuse gliomas are completely different from the adult type with respect to the molecular genetic characteristics, prognosis, and treatment strategies. pDLGG includes four tumor types, namely, diffuse astrocytoma, MYB- or MYBL1-altered; angiocentric glioma; polymorphous low-grade neuroepithelial tumor of the young (PLNTY); and diffuse low-grade glioma, MAPK pathway-altered, three types of which are newly recognized tumor types. Herein, we review the clinical characteristics, histopathological and molecular genetic characteristics, neuroimaging features, and prognosis of pDLGG and summarize the neuroimaging key points in diagnosing different tumor types. This review aims to evaluate and update the relevant pDLGG features and advances in neuroimaging that may assist in differential diagnosis, surgery planning, and prognostic determination of these tumor types and provide accurate diagnostic information for clinical colleagues.
Novel hub genes and regulatory network related to ferroptosis in tetralogy of Fallot
Authors: Wang Yu , Yang Junjie , Lu Jieru , Wang Qingjie , Wang Jian , Zhao Jianyuan , Huang Yuqiang , Sun Kun
Published: October 2023

Ferroptosis is a newly discovered type of cell death mainly triggered by uncontrolled lipid peroxidation, and it could potentially have a significant impact on the development and progression of tetralogy of Fallot (TOF). Our project aims to identify and validate potential genes related to ferroptosis in TOF. We obtained sequencing data of TOF from the GEO database and ferroptosis-related genes from the ferroptosis database. We employed bioinformatics methods to analyze the differentially expressed mRNAs (DEmRNAs) and microRNAs between the normal control group and TOF group and identify DEmRNAs related to ferroptosis. Protein–protein interaction analysis was conducted to screen hub genes. Furthermore, a miRNA–mRNA–TF co-regulatory network was constructed to utilize prediction software. The expression of hub genes was further validated through quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR). After conducting the differential gene analysis, we observed that in TOF, 41 upregulated mRNAs and three downregulated mRNAs associated with ferroptosis genes were found. Further Gene Ontology/Kyoto Encyclopedia of Genes and Genomes analysis revealed that these genes were primarily involved in molecular functions and biological processes related to chemical stress, oxidative stress, cellular response to starvation, response to nutrient levels, cellular response to external stimulus, and cellular response to extracellular stimulus. Furthermore, we constructed a miRNA–mRNA–TF co-regulatory network. qRT-PCR analysis of the right ventricular tissues from human cases showed an upregulation in the mRNA levels of KEAP1 and SQSTM1. Our bioinformatics analysis successfully identified 44 potential genes that are associated with ferroptosis in TOF. This finding significantly contributes to our understanding of the molecular mechanisms underlying the development of TOF. Moreover, these findings have the potential to open new avenues for the development of innovative therapeutic approaches for the treatment of this condition.
Evaluation of new generation systemic immune-inflammation markers to predict urine culture growth in urinary tract infection in children
Authors: Elgormus Yusuf , Okuyan Omer , Dumur Seyma , Sayili Ugurcan , Uzun Hafize
Published: October 2023

Objective

Systemic inflammation has been implicated in the development and progression of urinary tract infection (UTI). Accordingly, the aim of this study is to determine whether the white blood cell (WBC), C-reactive protein (CRP), neutrophil–lymphocyte ratio (NLR), platelet–lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) are useful markers to predict of urine culture growth in children with UTI. The second aim of this study is to evaluate the prevalence of UTI pathogens, antibiotic resistance patterns, and empirical treatment options in children diagnosed with UTI based on laboratory and clinical findings.

Method

The study population comprised 413 cases (positive urine culture) and 318 cases (negative urine culture) of pediatric patients with UTI.

Results

There was no statistically significant difference observed in the median levels of hemoglobin, hematocrit, and platelet between the negative and positive culture groups. The median levels of monocytes, WBC, NLR, SII, and CRP of the patients with a positive urine culture were shown to be statistically significantly higher than the patients with a negative urine culture. The AUC value was 0.747 (0.710–0.784) for CRP with a cutoff value of 3.2, the sensitivity value was 56.4%, and the specificity value was 98.4% in terms of UTI. The AUC value was 0.733 (0.697–0.769) for SII with a cutoff value of 600, the sensitivity value was 58.4%, and the specificity value was 83.0%. The AUC value was 0.732 (0.697–0.769) for NLR with a cutoff value of 2, the sensitivity value was 57.4%, and the specificity value was 81.1%.

Conclusion

WBC, CRP, NLR, PLR, and SII could potentially serve as useful independent diagnostic or complementary markers for disease in children diagnosed with UTI who exhibit a positive urine culture. Escherichia coli was found to be the most common causative agent, and the commonly prescribed antibiotic was cephalosporin. However, it was observed that all identified agents of pediatric UTIs in our center exhibited high resistance to cefuroxime, trimethoprim–sulfamethoxazole, cefixime, ampicillin, and ceftriaxone.
Individual and environmental parameters in children with and without developmental coordination disorder: associations with physical activity and body mass index
Authors: Valentini Nadia Cristina , Souza Priscila Silva de , Souza Mariele Santayana de , Nobre Glauber Carvalho
Published: October 2023

Introduction

Physical Activity (PA) is a complex behavior, and the relevance of other factors such as BMI, SES and children’s behavior at school and home still lacks investigation for children, especially those at risk or with Developmental Coordination Disorder (DCD). The objective of this study was to examine whether socioeconomic status (SES), school's spaces for children’s movement, active play, screen time, motor skill proficiency, perceived motor competence, and engagement in the physical education lessons were associated with PA and BMI in children with typical development (TD), at risk of DCD (r-DCD), and with DCD.

Methods

Children (N = 352; 4–10-year-old) from six public schools in a major urban city, in southern of Brazil, were assessed regarding motor skill proficiency (locomotor and ball skills), perceived motor competence, and weight status. PA and engagement in the lesson were assessed using pedometers and a behavior checklist of motor experience of success. Parents recorded the daily time that children spent on screen and in active play.

Results

The hierarchical multivariate linear regressions showed that age, sex, SES, Schools with more favorable space for children’s movement, locomotor and ball skills, and successful engagement, were associated with PA for children with TD. Age, screen time, locomotor, and successful engagement were associated with BMI. For children at r-DCD, age, sex, SES, with more favorable space for children’s movement, and locomotor were associated with PA. Age, active play and screen time were associated with BMI. For children with DCD, sex, SES, Schools with more favorable space for children’s movement, screen time, and successful engagement were associated with PA. Age, sex, active play, and screen time were associated with BMI.

Conclusion

Different factors were associated with PA and BMI for children with different levels of motor impairment (r-DCD and DCD) and children with TD.
Dynamic displacement estimation of structures using one-dimensional convolutional neural network
Authors: Zhou Xin , He Yuanpeng
Published: October 2023

For large infrastructures, dynamic displacement measurement in structures is an essential topic. However, limitations imposed by the installation location of the displacement sensor can lead to measurement difficulties. Accelerometers are characterized by easy installation, good stability and high sensitivity. For this regard, this paper proposes a structural dynamic displacement estimation method based on a one-dimensional convolutional neural network and acceleration data. It models the complex relationship between acceleration signals and dynamic displacement information. In order to verify the reliability of the proposed method, a finite element-based frame structure was created. Accelerations and displacements were collected for each node of the frame model under seismic response. Then, a dynamic displacement estimation dataset is constructed using the acceleration time series signal as features and the displacement signal at a certain moment as target. In addition, a typical neural network was used for a comparative study. The results indicated that the error of the neural network model in the dynamic displacement estimation task was 9.52 times higher than that of the one-dimensional convolutional neural network model. Meanwhile, the proposed modelling scheme has stronger noise immunity. In order to validate the utility of the proposed method, data from a real frame structure was collected. The test results showed that the proposed method has a mean square error of only 5.097 in the real dynamic displacement estimation task, which meets the engineering needs. Afterwards, the outputs of each layer in the dynamic displacement estimation model are visualized to emphasize the displacement calculation process of the convolutional neural network.
Identification of apple leaf disease via novel attention mechanism based convolutional neural network
Authors: Cheng Hebin , Li Heming
Published: October 2023

Introduction

The identification of apple leaf diseases is crucial for apple production.

Methods

To assist farmers in promptly recognizing leaf diseases in apple trees, we propose a novel attention mechanism. Building upon this mechanism and MobileNet v3, we introduce a new deep learning network.

Results and discussion

Applying this network to our carefully curated dataset, we achieved an impressive accuracy of 98.7% in identifying apple leaf diseases, surpassing similar models such as EfficientNet-B0, ResNet-34, and DenseNet-121. Furthermore, the precision, recall, and f1-score of our model also outperform these models, while maintaining the advantages of fewer parameters and less computational consumption of the MobileNet network. Therefore, our model has the potential in other similar application scenarios and has broad prospects.
Findings from the expert-novice paradigm on differential response behavior among multiple-choice items of a pedagogical content knowledge test – implications for test development
Authors: Lieberei Tobias , Welter Virginia Deborah Elaine , Großmann Leroy , Krell Moritz
Published: October 2023

Pedagogical content knowledge (PCK) is one core dimension of teachers’ professional knowledge and comprises knowledge about conceptual ideas of learners and appropriate instructions. However, several challenges regarding the assessment of PCK are discussed in the literature: For example, PCK is a topic-specific construct and contains differentiable subdomains, which must be considered during test development. In addition, the choice of test type needs to be considered. While open-ended instruments can capture a broader range of cognitions, they often require a high level of interpretation; in contrast, multiple-choice instruments have advantages in terms of objectivity and test economy. Some challenges of assessing PCK are particularly related to multiple-choice instruments, such as an insufficient focus on specific components or the accidental assessment of teachers’ beliefs instead of PCK. To better understand and explain these challenges in developing multiple-choice PCK instruments, we exemparly used an instrument to assess PCK about scientific reasoning and considered the assumptions of the expert-novice paradigm to analyze differential response behavior betweenn = 10 researchers in the field of biology education (experts) andn = 10 undergraduate pre-service biology teachers (novices). As expected, experts scored significantly higher than novices. At the same time, experts answered the items more consistently than novices, i.e., showed less variance. However, the difference found was statistically insignificant. Regarding the explanations for choosing a response option, experts more often correctly identified the quintessence of the items, which means that they more often understand the items as intended and argued based on their PCK. On the other hand, novices focused more on surface characteristics, i.e., they argued rather with surface knowledge like intuition or personal experience, than choosing the response option based on their PCK. These crucial differences in how experts and novices understand the items of the used PCK instrument and how they respond based on their understanding affect different test characteristics. In conclusion, we recommend ensuring that instruments address only a few, specific PCK aspects, considering the target group of a test, and take into account that target groups with larger variability among their responses require a higher number of items to achieve satisfactory discrimination and reliability indices.
Measuring the impact of an integrated bite case management program on the detection of canine rabies cases in Vietnam
Authors: Ross Yasmeen B. , Vo Chuong Dinh , Bonaparte Sarah , Phan Minh Quang , Nguyen Diep Thi , Nguyen Thin Xuan , Nguyen Thanh Tat , Orciari Lillian , Nguyen Tho Dang , Nguyen Oanh Kim Thi , Do Trang Thuy , Dao Anh Thi Phuong ,   REACT Development Team, Wallace Ryan , Nguyen Long Van
Published: October 2023

Introduction

Dog-mediated rabies is enzootic in Vietnam, resulting in at least 70 reported human deaths and 500,000 human rabies exposures annually. In 2016, an integrated bite cases management (IBCM) based surveillance program was developed to improve knowledge of the dog-mediated rabies burden in Phu Tho Province of Vietnam.

Methods

The Vietnam Animal Rabies Surveillance Program (VARSP) was established in four stages: (1) Laboratory development, (2) Training of community One Health workers, (3) Paper-based-reporting (VARSP 1.0), and (4) Electronic case reporting (VARSP 2.0). Investigation and diagnostic data collected from March 2016 to December 2019 were compared with historical records of animal rabies cases dating back to January 2012. A risk analysis was conducted to evaluate the probability of a rabies exposure resulting in death after a dog bite, based on data collected over the course of an IBCM investigation.

Results

Prior to the implementation of VARSP, between 2012 and 2015, there was an average of one rabies investigation per year, resulting in two confirmed and two probable animal rabies cases. During the 46 months that VARSP was operational (2016 – 2019), 1048 animal investigations were conducted, which identified 79 (8%) laboratory-confirmed rabies cases and 233 (22%) clinically-confirmed(probable) cases. VARSP produced a 78-fold increase in annual animal rabies case detection (one cases detected per year pre-VARSP vs 78 cases per year under VARSP). The risk of succumbing to rabies for bite victims of apparently healthy dogs available for home quarantine, was three deaths for every 10,000 untreated exposures.

Discussion

A pilot IBCM model used in Phu Tho Province showed promising results for improving rabies surveillance, with a 26-fold increase in annual case detection after implementation of a One Health model. The risk for a person bitten by an apparently healthy dog to develop rabies in the absence of rabies PEP was very low, which supports the WHO recommendations to delay PEP for this category of bite victims, when trained animal assessors are available and routinely communicate with the medical sector. Recent adoption of an electronic IBCM system is likely to expedite adoption of VARSP 2.0 to other Provinces and improve accuracy of field decisions and data collection.
Exploring the national prevalence of mental health risk, multimorbidity and the associations thereof: a repeated cross-sectional panel study
Authors: Craig Ashleigh , Mapanga Witness , Mtintsilana Asanda , Dlamini Siphiwe , Norris Shane
Published: October 2023

Objective and methods

South Africans were affected by the COVID-19 pandemic and resultant economic hardships. As a result, mental health within this region may have worsened. Therefore, using large scale nationally representative data, we repeated the cross-sectional panel study to investigate mental health risk post COVID-19 to explore mental health and multimorbidity and to examine the relationship between adverse childhood experiences (ACEs) and comorbid health conditions in South African adults (aged 18 years and older).

Results

Post-pandemic, 26.2, 17.0, and 14.8% of the South African respondents reported being probably depressed, anxious and had suffered high exposure to early life adversity, respectively. Nationally, the prevalence of mental health across the country remained alarmingly high when compared to Panel 1. The prevalence of multimorbidity (2 or more chronic morbidities) among the South African population was reported at 13.9%, and those with 2 or more morbidities were found to have increased odds of early adversity, irrespective of differing socio-demographics. Furthermore, early adversity was also associated with multimorbidity partly via mental health.

Conclusion

This repeated cross-sectional national study reiterated that the prevalence of mental health across South African adults aged 18 years and older is widespread. Mental health remains worryingly high post-pandemic where more than a quarter of respondents are probably depressed, nearly one in every five respondents are anxious, and 14.8% reported high exposure ACEs. Public health interventions need to be upscaled with efforts to reduce the incidence of early adversity that may have the ability to lower adverse health outcomes and mental ill-health in adulthood.
Mental health, study skills, social support, and barriers to seeking psychological help among university students: a call for mental health support in higher education
Authors: Hyseni Duraku Zamira , Davis Holly , Hamiti Era
Published: October 2023

Introduction

Poor mental health among youths is a complex worldwide issue. Many countries with medium-to-low levels of development, particularly those in Southern Europe, have not introduced appropriate mental health and educational strategies to identify the key factors influencing wellbeing, promote psychological wellbeing, and prevent poor mental health among youths. In response to these trends, we sought to uncover insights for developing interventions for youth mental wellbeing. We assessed mental health, study skills, barriers to seeking psychological help, and perceived social support among Kosovar university students, and investigated their experiences with professional mental health services and their needs and perceptions regarding the importance of professional mental health services on campus.

Methods

The study used a parallel mixed-methods design. Participants included 234 university students. Quantitative data were gathered through validated questionnaires, including the Depression, Anxiety, and Stress Scale, Multidimensional Scale of Perceived Social Support, Academic Anxiety Scale, Study Skills Assessment Questionnaire, and the Barriers to Seeking Psychological Help Scale. Qualitative data on the students’ experiences with mental health services and their perceptions regarding the importance of professional university mental health services were explored through open-ended questions.

Results

Most students experienced anxiety and depression, more than half were stressed, and most reported poor or moderate study skills. Lack of trust in mental health professionals was a major barrier to seeking psychological help, followed by difficulties in self-disclosure. Perceived social support and academic anxiety were significant predictors of barriers to seeking psychological help. The participants believed that mental health and academic support from the university would help improve their mental wellbeing, study skills, self-esteem, self-perception, and attitudes toward social support; raise awareness regarding mental health; and help them overcome personal and academic challenges.

Discussion

Our findings highlight the need for more comprehensive and accessible mental health services on campuses. By providing adequate support and resources to address various personal and academic factors that contribute to mental health issues in university students, universities can enhance students’ academic success and personal growth.
Epidemiological characteristics of HIV transmission in southeastern China from 2015 to 2020 based on HIV molecular network
Authors: Wang Zhenghua , Wang Dong , Lin Liying , Qiu Yuefeng , Zhang Chunyan , Xie Meirong , Lu Xiaoli , Lian Qiaolin , Yan Pingping , Chen Liang , Feng Yi , Xing Hui , Wang Wei , Wu Shouli
Published: October 2023

Objective

HIV/AIDS remains a global public health problem, and understanding the structure of social networks of people living with HIV/AIDS is of great importance to unravel HIV transmission, propose precision control and reduce new infections. This study aimed to investigate the epidemiological characteristics of HIV transmission in Fujian province, southeastern China from 2015 to 2020 based on HIV molecular network.

Methods

Newly diagnosed, treatment-naive HIV/AIDS patients were randomly sampled from Fujian province in 2015 and 2020. Plasma was sampled for in-house genotyping resistance test, and HIV molecular network was created using the HIV-TRACE tool. Factors affecting the inclusion of variables in the HIV molecular network were identified using univariate and multivariate logistic regression analyses.

Results

A total of 1,714 eligible cases were finally recruited, including 806 cases in 2015 and 908 cases in 2020. The dominant HIV subtypes were CRF01_AE (41.7%) and CRF07_BC (38.3%) in 2015 and CRF07_BC (53. 3%) and CRF01_AE (29.1%) in 2020, and the prevalence of HIV drug resistance was 4.2% in 2015 and 5.3% in 2020. Sequences of CRF07_BC formed the largest HIV-1 transmission cluster at a genetic distance threshold of both 1.5 and 0.5%. Univariate and multivariate logistic regression analyses showed that ages of under 20 years and over 60 years, CRF07_BC subtype, Han ethnicity, sampling in 2015, absence of HIV drug resistance, married with spouse, sampling from three cities of Jinjiang, Nanping and Quanzhou resulted in higher proportions of sequences included in the HIV transmission molecular network at a genetic distance threshold of 1.5% (p < 0.05).

Conclusion

Our findings unravel the HIV molecular transmission network of newly diagnosed HIV/AIDS patients in Fujian province, southeastern China, which facilitates the understanding of HIV transmission patterns in the province.
Prevalence, correlates, and trends of intimate partner violence against women in Sierra Leone: findings from 2013 and 2019 demographic and health surveys
Authors: Shaikh Masood Ali
Published: October 2023

Background

Intimate partner violence (IPV) is a globally pervasive public health and medical problem in addition to being a serious violation of women’s rights. The two-fold objectives of this study were to compute the lifetime prevalence and correlates of IPV perpetrated by current/former husbands/partners of ever-married women aged 15–49 years using the nationally representative Sierra Leone Demographic and Health Survey (DHS) conducted in 2019; ethnicity-based levels and trends of IPV were also computed using the data from the DHS conducted in 2013 along with the 2019 DHS.

Methods

Twelve IPV correlates pertaining to socio-demographic, attitudinal, and experiential attributes were analyzed using logistic regression models for bivariate and multivariable analysis. IPV was computed as a composite variable comprising of physical, emotional, and/or sexual IPV.

Results

Lifetime prevalence of experiencing any kind of IPV was a staggering 60.81%, whereas emotional, physical, and sexual IPV prevalence were reported by 45.90%, 49.81%, and 8.14% of the respondents, respectively. No ethnicity reported a statistically significant decrease in any type of IPV during the intervening period between the two surveys. Five out of nine ethnicities reported a statistically significant increase in emotional IPV, while few ethnicities reported a statistically significant increase in one or more types of IPV.

Conclusion

The high lifetime prevalence of IPV is alarming and points to the need for targeted health promotion campaigns to reverse the tide of IPV in Sierra Leone, including focusing on changes in cultural and ethnicity-based norms and mores to ensure women’s human rights are respected and upheld.
Intercultural adaptation and influencing factors analysis of the Chinese version of the anxiety scale for the older adults in a long-term care population
Authors: Feng Fuzhe , Chen Qing , Zheng Chen , Zhang Huijun
Published: October 2023

Objective

The purpose of this study was to translate the Geriatric Anxiety Scale-Long-Term Care into Chinese and to assess its reliability and validity in a long-term care population, as well as to explore factors contributing to anxiety in older adults requiring long-term care.

Methods

The study recruited 399 older adults residents requiring long-term care and used the Brislin double translation-back-translation method to create the initial Chinese version of the Geriatric Anxiety Scale-Long-Term Care. The study used internal consistency and split-half reliability to assess the reliability of the scale, as well as exploratory factor analysis, validation factor analysis, and content validity to assess the validity of the scale. Linear regression was used to analyze the relationship between the independent variables and anxiety levels in the long-term care population.

Results

The Cronbach’s coefficient value of the Chinese version of the Geriatric Anxiety Scale-Long-Term Care was 0.81, and the split-half reliability was 0.80. The results of exploratory factor analysis showed support for a one-dimensional factor structure. The results of the validation factor analysis indicated a good fit for the one-factor model. Gender (β = 0.190, 95% CI:0.540 ~ 1.546, p < 0.001), self-rated health (β = 0.220, 95% CI:0.379 ~ 0.953, p < 0.001), life satisfaction (β = −0.315, 95% CI: −1.355 ~ −0.734, p < 0.001) and participation in activities (β = −0.106, 95% CI: −1.122 ~ −0.084, p < 0.05) were significant predictors of anxiety levels in the long-term care population.

Conclusion

The Chinese version of the Geriatric Anxiety Scale-Long-Term Care has good reliability and validity in the long-term care population. The Geriatric Anxiety Scale-Long-Term Care is effective in assessing the anxiety level of the Chinese long-term care older adults population and provides an opportunity to detect and observe anxiety disorders in the long-term care population.
The rationale behind updates to ambient ozone guidelines and standards
Authors: Xue Kaibing , Zhang Xin
Published: October 2023

Although air quality has gradually improved in recent years, as shown by the decrease in PM2.5 concentration, the problem of rising ambient ozone has become increasingly serious. To reduce hazards to human health and environmental welfare exposure to ozone, scientists and government regulators have developed ozone guidelines and standards. These answer the questions of which levels of exposure are hazardous to human health and the environment, and how can ambient ozone exposure be guaranteed, respectively. So what are the basis for the ozone guidelines and standards? This paper reviews in detail the process of revising ozone guidelines and standards by the World Health Organization (WHO) and the United States Environmental Protection Agency (EPA). The present study attempts to explore and analyze the scientific basis and empirical methods for updating guidelines and standards, in a view to guide the future revision process and provide directions for further scientific research. We found many epidemiological and toxicological studies and exposure-response relationships provided strong support for developing and revising the ozone guidelines. When setting standards, ozone exposure has been effectively considered, and the economic costs, health, and indirect economic benefits of standard compliance were reasonably estimated. Accordingly, epidemiological and toxicological studies and the establishment of exposure-response relationships, as well as exposure and risk assessment and benefit-cost estimates of standards compliance should be strengthened for the further update of guidelines and standards. In addition, with the increasing prominence of combined air pollution led by ozone and PM2.5, more joint exposure scientific research related to ozone guidelines and standards should be undertaken.
Bioinspired robots can foster nature conservation
Authors: Chellapurath Mrudul , Khandelwal Pranav C. , Schulz Andrew K.
Published: October 2023

We live in a time of unprecedented scientific and human progress while being increasingly aware of its negative impacts on our planet’s health. Aerial, terrestrial, and aquatic ecosystems have significantly declined putting us on course to a sixth mass extinction event. Nonetheless, the advances made in science, engineering, and technology have given us the opportunity to reverse some of our ecosystem damage and preserve them through conservation efforts around the world. However, current conservation efforts are primarily human led with assistance from conventional robotic systems which limit their scope and effectiveness, along with negatively impacting the surroundings. In this perspective, we present the field of bioinspired robotics to develop versatile agents for future conservation efforts that can operate in the natural environment while minimizing the disturbance/impact to its inhabitants and the environment’s natural state. We provide an operational and environmental framework that should be considered while developing bioinspired robots for conservation. These considerations go beyond addressing the challenges of human-led conservation efforts and leverage the advancements in the field of materials, intelligence, and energy harvesting, to make bioinspired robots move and sense like animals. In doing so, it makes bioinspired robots an attractive, non-invasive, sustainable, and effective conservation tool for exploration, data collection, intervention, and maintenance tasks. Finally, we discuss the development of bioinspired robots in the context of collaboration, practicality, and applicability that would ensure their further development and widespread use to protect and preserve our natural world.
Can Viruses Be Used to Make People Healthy?
Authors: Levochkina Marina , Beryozkin Avigail , Aweidah Hamzah , Beale Oliver M. , Byrne Leah C.
Published: October 2023

Viruses are small particles that are so tiny they cannot be seen by a regular light microscope. However, viruses can be found almost anywhere you look. Viruses specialize in carrying genetic material into all kinds of cells, including human cells. Many viruses are harmless to humans while others can cause illnesses like the cold, the flu, or COVID-19. Recently, scientists have been using viruses to deliver genes into cells to cure human diseases! This is called gene therapy. Genes are the blueprints for the proteins that make up the human body, and mistakes in a gene can cause disease. Gene therapy is a way to treat these types of diseases. To create a gene therapy, scientists cut out a virus’s genes and replace them with properly working human genes. These modified viruses can then be used to deliver healthy genes and treat disease.