bluuebunny/crossref_metadata_2025 huggingface dataset:
Density Functional Theory Simulation of Dithienothiophen[3,2-b]-pyrrolobenzothiadiazole-Based Organic Solar Cells
Authors: Setsoafia Daniel Dodzi Yao , Ram Kiran Sreedhar , Mehdizadeh-Rad Hooman , Ompong David , Singh Jai
Published: January 2024

We have simulated the effect of changing the end groups in BTP core with five organic units of 1,3-Indandione (IN), 2-thioxothiazolidin-4-one (Rhodanine), propanedinitrile (Malononitrile), (2-(6-oxo-5,6-dihydro-4H-cyclopenta[c]thiophen-4-ylidene)malononitrile) (CPTCN) and 2-(3-oxo-2,3-dihydroinden-1-ylidene (IC), and two halogenated units of (4F) IC and (4Cl) IC on the optical and photovoltaic properties of the BTP DA’D core molecular unit. Thus modified, seven molecular structures are considered and their optical properties, including HOMO and LUMO energies and absorption spectra are simulated in this paper. On the basis of HOMO and LUMO energies, it is found that two of the seven molecules, BTP-IN and BTP-Rhodanine, can act as donors and the other four, BTP-(4F) IC, BTP-(4Cl) IC, BTP-CPTCN and BTP-IC, as acceptors in designing bulk heterojunction (BHJ) organic solar cells (OSCs). Using these combinations of donors and acceptors in the active layer, eight BHJ OSCs, such as BTP-IN: BTP-(4F) IC, BTP-IN: BTP-(4Cl) IC, BTP-IN: BTP-CPTCN, BTP-IN: BTP-IC, BTP-Rhodanine: BTP-(4F) IC, BTP-Rhodanine: BTP-(4Cl) IC, BTP-Rhodanine: BTP-CPTCN and BTP-Rhodanine: BTP-IC, are designed, and their photovoltaic performance is simulated. The photovoltaic parameters Jsc, Voc and FF for all eight BHJ OSCs and their power conversion efficiency (PCE) are simulated. It is found that the BHJ OSC of the BTP-IN: BTP-CPTCN donor–acceptor blend gives the highest PCE (14.73%) and that of BTP-Rhodanine: BTP-(4F) IC gives the lowest PCE (12.07%). These results offer promising prospects for the fabrication of high-efficiency BHJ OSCs with the blend of both donor and acceptor based on the same core structure.
A Forest Fire Recognition Method Based on Modified Deep CNN Model
Authors: Zheng Shaoxiong , Zou Xiangjun , Gao Peng , Zhang Qin , Hu Fei , Zhou Yufei , Wu Zepeng , Wang Weixing , Chen Shihong
Published: January 2024

Controlling and extinguishing spreading forest fires is a challenging task that often leads to irreversible losses. Moreover, large-scale forest fires generate smoke and dust, causing environmental pollution and posing potential threats to human life. In this study, we introduce a modified deep convolutional neural network model (MDCNN) designed for the recognition and localization of fire in video imagery, employing a deep learning-based recognition approach. We apply transfer learning to refine the model and adapt it for the specific task of fire image recognition. To combat the issue of imprecise detection of flame characteristics, which are prone to misidentification, we integrate a deep CNN with an original feature fusion algorithm. We compile a diverse set of fire and non-fire scenarios to construct a training dataset of flame images, which is then employed to calibrate the model for enhanced flame detection accuracy. The proposed MDCNN model demonstrates a low false alarm rate of 0.563%, a false positive rate of 12.7%, a false negative rate of 5.3%, and a recall rate of 95.4%, and achieves an overall accuracy of 95.8%. The experimental results demonstrate that this method significantly improves the accuracy of flame recognition. The achieved recognition results indicate the model’s strong generalization ability.
Swelling Behaviour of Bamboo (Phyllostachys pubescens)
Authors: Roszyk Edward , Kropaczewski Radosław , Mania Przemysław , Broda Magdalena
Published: January 2024

Bamboo is a plant with various applications. As a natural, renewable material that exhibits good mechanical performance, it seems to be an interesting alternative to wood, which has become a scarce and expensive commodity. However, comprehensive knowledge of its properties is necessary to maximise its potential for various industrial purposes. The swelling behaviour of bamboo is one of the features that has not yet been sufficiently investigated. Therefore, in this research, we aimed to measure and analyse the swelling pressure and kinetics of bamboo blocks. The results show that similar to wood, the swelling kinetics of bamboo depend on its density: the denser the tissue, the higher the maximum swelling value recorded. The maximum tangential swelling measured was about 5%–6%, which is lower than the value for the most commonly used wood species. Swelling pressure ranged from 1.16 MPa to 1.39 MPa, depending on the bamboo density: the denser the sample, the shorter the time required to reach maximum swelling pressure. Like in wood, the smallest linear increase in size due to swelling was observed in the longitudinal direction (0.71%). However, opposite to wood, more pronounced swelling was recorded in the radial direction (over 7%) than in the tangential direction (nearly 6%). The results show that bamboo’s swelling behaviour makes it a good material for use in variable humidity conditions, being more favourable than the unmodified wood of many species.
The Minimum Target Diameter and the Harvest Age of Oak Natural Secondary Forests in Different Sites Conditions: Case Study in Hunan Province, China
Authors: You Wenbiao , Zhu Guangyu
Published: January 2024

Maintaining permanent forest canopy cover and eventually harvesting timber by predetermined target diameter are often considered as a prototype for future management of the oak natural forest. However, target diameters and harvest age based on average forest growth rates from wide geographical areas often hamper improved management of oak forests. In this study, based on the sampling of 129 target trees from 51 oak natural secondary forest plots in Hunan Province, China, an individual-tree DBH (diameter at breast height) growth model of oak target trees was developed, and the site type (41 levels) was related to the model as random effects by a nonlinear mixed-effects approach. Moreover, the 41 site types were clustered into four site type groups (STG1, STG2, STG3, and STG4) by the K-means clustering algorithm to improve the model performance and practicality. With the help of the model, the five target diameters (including 24, 30, 40, 50, and 60 cm) were simulated in each of the four STGs, and the minimum target diameter was determined for each STG based on the theory of quantitative maturity. In the four STGs, the harvest age of the 24 cm diameter target ranged from 30 to 51 years; the harvest age of the 60 cm target diameter ranged from 131 to 220 years, with the oaks failing to reach 60 cm in the lowest-quality STG4; the minimum target diameter ranged from 21 cm to 29 cm. Results showed that lower-quality sites exclude higher target diameters from optimal harvesting strategies, in contrast to the higher target diameter as a more reasonable strategy in higher quality sites, and that the minimum target diameter is significantly influenced by site conditions. Therefore, it is necessary to develop a diverse target-diameter-harvesting strategy adapted for the complex site conditions of oak forests in Hunan Province towards site-specific timber management to improve the sustainability of timber production in oak forests.
The Global Situation of Karst Desertification Research Based on Forest Ecology
Authors: Zhang Yu , Zhang Zhaohui , Zhang Mingsheng , Yuan Zhouwei
Published: January 2024

Karst desertification (KD) is a major ecological and environmental problem threatening human survival and development in karst areas. In order to explore the research situation and development trend of KD based on forest ecology, this paper provides a systematic literature review based on the CNKI and WoS databases, which involves search, appraisal, synthesis, and analysis. We performed a statistical and visual analysis of 2955 studies acquired between 1 January 1995 and 30 June 2023, including the time distribution of the studies, hot research trends, pivotal research clusters, literature co-citations, main publishing trends, and keyword bursts. The results show the following. (1) The literature shows a fluctuating growth trend, and the research trends are divided into accumulation, development, and expansion stages. (2) The pivotal research clusters comprised three major aspects: study area and method, driving and genetic mechanisms, and control technology and models. (3) KD research forms a China-centered research network, dominated by institutions and scholars in southwestern China. (4) Future research needs to solve a series of related scientific issues and technological needs for forest ecosystem function optimization, ecological product supply capacity enhancement, ecological product value realization, ecological industry formation, and rural revitalization in KD control. The purpose of this paper is to reveal research trends relating to KD and highlight the future direction of KD research and control.
The Range Potential of North American Tree Species in Europe
Authors: Albrecht Axel Tim , Heinen Henry , Koch Olef , de Avila Angela Luciana , Hinze Jonas
Published: January 2024

European forest ecosystems are projected to change severely under climate change especially due to an anticipated decline in the distribution of major tree species in Europe. Therefore, the adaptation of European forests appears necessary and urgent. While spontaneous adaptation mechanisms bear a large self-guided potential, we focus on quantifying the potential of management-guided mechanisms. Besides other possible tree species groups for adaptation, non-native tree species from North America have a long tradition in Europe, yet their full distribution potential is not completely revealed. We applied an ensemble species distribution model approach to six North American species, using combined occurrence data from the native and naturalized ranges to gain more insights into the species suitability in the introduced area in 2070 (2061–2080) under the emission scenarios RCP 4.5 and 8.5. Our findings support the assumption that there is unreported species potential in the introduced area beyond their current distribution. Next to northeastern range shifts projected for all species, we identified Abies grandis, Liriodendron tulipifera, Quercus rubra, and Robinia pseudoacacia with increasing range potentials in the future. P. ponderosa and P. menziesii var. menziesii are projected to show a steady and decreased range potential under RCP 4.5 and 8.5, respectively.
Family Income Level, Income Structure, and Dietary Imbalance of Elderly Households in Rural China
Authors: Wang Gangyi , Hao Yanzhi , Ma Jiwei
Published: January 2024

In rural areas, the aging of households is becoming increasingly severe, and the issue of dietary imbalance among the elderly is becoming increasingly prominent. Using data from the China Health and Nutrition Survey (CHNS), the negative binomial regression method was used to investigate the impact of household income level on dietary imbalance among rural elderly people, and to explore the heterogeneity of household income structure and its role in the relationship between the two. Research has found that an increase in total household income significantly improves the dietary quality of rural elderly people, and the income structure variable enhances its negative pulling effect on dietary imbalance. For elderly people with moderate dietary imbalance, the effect of increased family income is most significant. In different income groups, the impact of total household income on dietary imbalance in the high-income group is greater than that in the low-income group, and there is also a significant difference in the role played by the proportion of net income from agricultural operations. Therefore, it is necessary to increase the non working income of the elderly, strengthen social responsibility for elderly care, and alleviate the problem of dietary imbalance among rural elderly people.
A Literature Review of the Pharmacological Effects of Jujube
Authors: Zhu Deqi , Jiang Ning , Wang Ning , Zhao Yufen , Liu Xinmin
Published: January 2024

Jujube is a plant native to China that could be used in medicine and food. Its dried fruit is a superior herb commonly used in traditional Chinese medicine formulations for its calming effect and for nourishing the blood and strengthening the spleen and stomach. Jujube contains numerous active components including polysaccharides, phenols, and triterpene acids, which show a diverse array of pharmacological activities such as neuroprotection and the prevention and treatment of cardiovascular diseases. In this paper, the research status of jujube over the past two decades has been statistically evaluated. Meanwhile, by tracking the latest research advances, the pharmacological efficacy and molecular mechanisms of jujube are exhaustively expounded to provide specific and systematic references for further research on the pharmacological effects of jujube and its application in the food and pharmaceutical industries.
Reconnaissance of the Effects of the MW5.7 (ML6.4) Jajarkot Nepal Earthquake of 3 November 2023, Post-Earthquake Responses, and Associated Lessons to Be Learned
Authors: Subedi Mandip , KC Rajan , Sharma Keshab , Misra Jibendra , KC Apil
Published: January 2024

On 3 November 2023, a moment magnitude (MW) 5.7 (Local Magnitude, ML6.4) earthquake struck the western region of Nepal, one of the most powerful seismic events since 1505 in the region. Even though the earthquake was of moderate magnitude, it caused significant damage to several masonry buildings and caused slope failures in some regions. The field reconnaissance carried out on 6–9 November by the study team, following the earthquake, conducted the first-hand preliminary damage assessment in the three most affected districts—Jajarkot; West Rukum; and Salyan. This study covers the observed typical structural failures and geotechnical case studies from the field study. To have a robust background understanding, this paper examines the seismotectonic setting and regional seismic activity in the region. The observations of earthquake damage suggest that most of the affected buildings were made of stone or brick masonry without seismic consideration, while most of the reinforced concrete (RC) buildings remained intact. Case histories of damaged buildings, the patterns, and the failure mechanisms are discussed briefly in this paper. Significant damage to Khalanga Durbar, a historical monument in Jajarkot, was also observed. Medium- to large-scale landslides and rockfalls were recorded along the highway. The motorable bridge in the Bheri River suffered from broken bolts, rotational movement at the expansion joint, and damage to the stoppers. The damage observations suggest that, despite the existence of building codes, their non-implementation could have contributed to the heavy impact in the region. This study highlights that the local population faces a potential threat of subsequent disasters arising from earthquakes and earthquake-induced landslides. This underscores the necessity for proactive measures in preparedness for future disasters.
Persistence of Low Back Pain and Predictive Ability of Pain Intensity and Disability in Daily Life among Nursery School Workers in Japan: A Five-Year Panel Study
Authors: Aoshima Megumi , Shi Xuliang , Iida Tadayuki , Hiruta Shuichi , Ono Yuichiro , Ota Atsuhiko
Published: January 2024

Nursery school workers are known for having a high prevalence of low back pain (LBP). The natural history of LBP and the determinants of persistent LBP remain unclear. We examined the prevalence of persistent LBP and whether pain intensity and disability in daily life due to LBP affected the persistence of LBP among these workers. A five-year panel study was conducted for 446 nursery school workers in Japan. LBP, pain intensity, and disability in daily life due to LBP were assessed with a self-administered questionnaire survey. Pain intensity was assessed using the numerical rating scale (NRS). The Roland–Morris Disability Questionnaire (RDQ) was used to assess disability in daily life due to LBP. At baseline, 270 nursery school workers (60.5%) suffered from LBP. The estimated prevalence of persistent LBP was 84.6% (80.3–88.9%), 82.2% (77.7–86.8%), and 82.0% (77.4–86.5%) at 1, 3, and 5 years after the initial study, respectively. NRS scores of 5 or greater predicted the persistence of LBP at 1 and 3 years after the initial survey (adjusted odds ratios: 4.01 (1.27–12.6) and 8.51 (1.87–38.7), respectively), while RDQ scores did not. In conclusion, LBP highly persisted for a long time and pain intensity predicted persistent LBP among nursery school workers in Japan.
Caregiver Experiences with Dementia-Related Feeding/Eating Difficulties
Authors: Sharma Shobha , A. Halim Nur Atiqah , Maniam Puspa
Published: January 2024

This cross-sectional study explores caregivers’ perceptions of feeding/eating difficulties in persons living with dementia, their support provisions, and the associated burdens. Cognitive decline, behavioral symptoms, and physical issues contribute to the deterioration of feeding/eating activities in people with dementia. Inadequate support during mealtimes has adverse consequences. This study includes 31 caregivers who completed an online questionnaire with three sections: sociodemographic information, feeding/eating problems and required support for individuals with dementia, and caregiver burden and distress. The questions on feeding and eating problems were adapted from the Appetite and Eating Habits Questionnaire (APEHQ). The results show that nearly all persons living with dementia had symptoms of feeding/eating problems, requiring caregiver support, ranging from verbal assistance to full physical assistance. The caregivers reported high distress, which was positively correlated with dementia severity. The findings emphasize the importance of raising caregiver awareness about dementia’s impact on eating behavior, identifying effective mealtime care strategies, meeting nutritional needs, and emphasizing personal self-care. This research provides insights for healthcare professionals to develop targeted interventions, alleviate caregiver burden, improve mealtime experiences, and ensure adequate nutrition for persons living with dementia.
The Relationships between Effortful Control, Mind Wandering, and Mobile Phone Addiction Based on Network Analysis
Authors: Qiu Rui , Guo Zhihua , Wang Xianyang , Wang Xinlu , Cheng Sizhe , Zhu Xia
Published: January 2024

Background: The prevailing mobile phone use brought the problem of addiction, which might cause negative consequences. Effortful control and mind wandering were associated with addictive behavior. The present study aimed to investigate the dimension-level relationships between effortful control, mind wandering, and mobile phone addiction. Methods: A total of 1684 participants participated this study. The mobile phone addiction, effortful control, and mind wandering were measured through self-report scales, respectively. Dimension-level network of these psychological variables was estimated and bridge expected influence (BEI) values for each node was calculated. Results: Dimensions of mobile phone addiction, effortful control, and mind wandering exhibited distinct and complex links to each other. The node “activation control” exhibited the highest negative BEI value (BEI = −0.32), whereas “spontaneous thinking” showed the highest positive BEI value (BEI = 0.20). Conclusions: Different dimensions of effortful control and mind wandering had varied yet significant connections with distinct dimensions of mobile phone addiction, facilitating understanding of the specific pathways underlying the three constructs. The identified dominant bridge nodes can provide potential targets for the intervention of mobile phone addiction.
Toward Innovation in Healthcare: An Analysis of the Digital Behavior of Older People in Europe and Japan for the Introduction of a Technological Coaching System
Authors: Möller Johanna , Stara Vera , Amabili Giulio , Barbarossa Federico , Riccardi Giovanni Renato , Martella Clotilda , Di Donna Valentina , Palmier Cecilia , Ogawa Toshimi , Luc Marine , Wieching Rainer , Maranesi Elvira , Bevilacqua Roberta
Published: January 2024

(1) Background: The increasing older population and demographic shifts highlight the need to understand the digital profiles of older adults, a pivotal factor in developing innovative technologies like the e-VITA virtual coach. This personalized coach provides recommendations for sustainable well-being in a smart home environment. (2) Methods: This study focuses on analyzing the characteristics of older individuals categorized as Internet users (onliners) and non-users (offliners). European Social Survey data from 2021 were utilized for European analysis, determining Internet usage based on frequency. Offliners are defined as users who never use the Internet, and onliners as those who use it, albeit with different frequencies. In Japan, data from the 9th International Comparative Survey on the Lives and Attitudes of the Elderly were employed, based on the responses of 1367 subjects, which defined onliners as individuals using communication devices and offliners as those not utilizing fax machines, cell phones, or the Internet. (3) Results: This paper presents a primary analysis of older end-user context and perspectives, outlining effective strategies for the diffusion of an active and healthy aging coaching system in the market and society. (4) Conclusions: the study emphasizes the importance of analyzing digital behavior in any user-centered design approach to ensure the system’s acceptance after deployment.
An Exploration of Pediatricians’ Professional Identities: A Q-Methodology Study
Authors: Tiao Mao-Meng , Chang Yu-Che , Ou Liang-Shiou , Hung Chi-Fa , Khwepeya Madalitso
Published: January 2024

Professional identities may influence a wide range of attitudes, ethical standards, professional commitments and patient safety. This study aimed to explore the important elements that comprise pediatricians’ professional identities. A Q-methodology was used to identify the similarities and differences in professional identity. Forty pediatricians were recruited from two tertiary referral hospitals in Taiwan. A list of statements was developed by five attending physicians and three residents. R software was used to analyze the Q-sorts to load the viewpoints and formulate the viewpoint arrays. Additional qualitative data—one-to-one personal interviews—were analyzed. Twenty-eight of forty pediatricians, 11 males and 17 females, with an average age of 39.9 (27–62) years, were associated with four viewpoints. We labeled the four viewpoints identified for professional identity as (1) professional recognition, (2) patient communication, (3) empathy and (4) insight. The professional recognition viewpoint comprised of youngest participants—28–36 years—with the majority as residents (77.8%), while the empathy viewpoint comprised the oldest participants—38–62 years—with all as attending physicians. All participants in the empathy and insight viewpoints were married. This study found professional identity to be a multifaceted concept for pediatricians, especially in the areas of professional recognition, patient communication, empathy and insight into patient care.
Characteristics of Gastroenteritis Outbreaks Investigated in Singapore: 2018–2021
Authors: Fua’di Muhd Tarmidzi , Er Benjamin , Lee Sylvester , Chan Pei Pei , Khoo Joanna , Tan Desmond , Li Huilin , Muhammad Imran Roshan , Raj Pream , Kurupatham Lalitha , Lee Vernon , Tan Li Kiang , Chan Joanne Sheot Harn , Li Angela , Aung Kyaw Thu
Published: January 2024

There is a need to study the characteristics of outbreaks via Singapore’s outbreak surveillance system to understand and identify the gaps in food safety for targeted policy interventions due to the increasing trend in gastroenteritis outbreaks and consequential increase in foodborne-related deaths and economic burden on public health systems worldwide. A total of 171 gastroenteritis outbreaks were investigated in Singapore from January 2018 to December 2021. This study analyzed the annual trend of investigated gastroenteritis outbreaks, the proportion of outbreaks by implicated sources of food, and the proportion of the type of pathogens identified from human cases, food samples, and environmental swabs collected from outbreak investigations. Among the foodborne gastroenteritis outbreaks (n = 121) investigated in Singapore, approximately 42.1% of the outbreaks had food prepared by caterers, 14.9% by restaurants, and 12.4% had food prepared by in-house kitchens. Clostridium perfringens and Salmonella were the most common causative pathogens in foodborne outbreaks throughout the analysis period. The food samples and environmental swabs collected were mostly detected for Bacillus cereus. Norovirus was the most common causative pathogen in non-foodborne outbreaks and was mainly attributable to preschools. This highlights the importance of monitoring and educating the catering industry and preschools to prevent future outbreaks.
Probabilistic Time Geographic Modeling Method Considering POI Semantics
Authors: Wang Ai-Sheng , Yin Zhang-Cai , Ying Shen
Published: January 2024

The possibility of moving objects accessing different types of points of interest (POIs) at specific times is not always the same, so quantitative time geography research needs to consider the actual POI semantic information, including POI attributes and time information. Existing methods allocate probabilities to position points, including POIs, based on space–time position information, but ignore the semantic information of POIs. The accessing activities of moving objects in different POIs usually have obvious time characteristics, such as dinner usually taking place around 6 PM. In this paper, building upon existing probabilistic time geographic methods, we introduce POI attributes and their time preferences to propose a probabilistic time geographic model for assigning probabilities to POI accesses. This model provides a comprehensive measure of position probability with space–time uncertainty between known trajectory points, incorporating time, space, and semantic information, thereby avoiding data gaps caused by single-dimensional information. Experimental results demonstrate the effectiveness of the proposed method.
Haplotype of the Lipoprotein(a) Gene Variants rs10455872 and rs3798220 Is Associated with Parameters of Coagulation, Fibrinolysis, and Inflammation in Patients after Myocardial Infarction and Highly Elevated Lipoprotein(a) Values
Authors: Ugovšek Sabina , Rehberger Likozar Andreja , Levstek Tina , Trebušak Podkrajšek Katarina , Zupan Janja , Šebeštjen Miran
Published: January 2024

Lipoprotein(a) (Lp(a)) is an independent risk factor for future coronary events. Variants rs10455872 and rs3798220 in the gene encoding Lp(a) are associated with an increased Lp(a) concentration and risk of coronary artery disease. We aimed to determine whether in high-risk coronary artery disease patients these two genetic variants and the kringle IV type 2 (KIV-2) repeats are associated with impairment of inflammatory and hemostatic parameters. Patients after myocardial infarction with elevated Lp(a) levels were included. Blood samples underwent biochemical and genetic analyses. In carriers of the AC haplotype, the concentrations of tumor necrosis factor (TNF)-α (4.46 vs. 3.91 ng/L, p = 0.046) and plasminogen activator inhibitor-1 (PAI-1) (p = 0.026) were significantly higher compared to non-carriers. The number of KIV-2 repeats was significantly associated with the concentration of high-sensitivity C-reactive protein (ρ = 0.251, p = 0.038) and overall fibrinolytic potential (r = −0.253, p = 0.038). In our patients, a direct association between the AC haplotype and both TNF-α and PAI-1 levels was observed. Our study shows that the number of KIV-2 repeats not only affects proatherosclerotic and proinflammatory effects of Lp(a) but is also associated with its antifibrinolytic properties.
Functional Roles of CD26/DPP4 in Bleomycin-Induced Pulmonary Hypertension Associated with Interstitial Lung Disease
Authors: Okaya Tadasu , Kawasaki Takeshi , Sato Shun , Koyanagi Yu , Tatsumi Koichiro , Hatano Ryo , Ohnuma Kei , Morimoto Chikao , Kasuya Yoshitoshi , Hasegawa Yoshinori , Ohara Osamu , Suzuki Takuji
Published: January 2024

Pulmonary hypertension (PH) with interstitial lung diseases (ILDs) often causes intractable conditions. CD26/Dipeptidyl peptidase-4 (DPP4) is expressed in lung constituent cells and may be related to the pathogenesis of various respiratory diseases. We aimed to clarify the functional roles of CD26/DPP4 in PH-ILD, paying particular attention to vascular smooth muscle cells (SMCs). Dpp4 knockout (Dpp4KO) and wild type (WT) mice were administered bleomycin (BLM) intraperitoneally to establish a PH-ILD model. The BLM-induced increase in the right ventricular systolic pressure and the right ventricular hypertrophy observed in WT mice were attenuated in Dpp4KO mice. The BLM-induced vascular muscularization in small pulmonary vessels in Dpp4KO mice was milder than that in WT mice. The viability of TGFβ-stimulated human pulmonary artery SMCs (hPASMCs) was lowered due to the DPP4 knockdown with small interfering RNA. According to the results of the transcriptome analysis, upregulated genes in hPASMCs with TGFβ treatment were related to pulmonary vascular SMC proliferation via the Notch, PI3K-Akt, and NFκB signaling pathways. Additionally, DPP4 knockdown in hPASMCs inhibited the pathways upregulated by TGFβ treatment. These results suggest that genetic deficiency of Dpp4 protects against BLM-induced PH-ILD by alleviating vascular remodeling, potentially through the exertion of an antiproliferative effect via inhibition of the TGFβ-related pathways in PASMCs.
Immunosenescence and Cytomegalovirus: Exploring Their Connection in the Context of Aging, Health, and Disease
Authors: Müller Ludmila , Di Benedetto Svetlana
Published: January 2024

Aging induces numerous physiological alterations, with immunosenescence emerging as a pivotal factor. This phenomenon has attracted both researchers and clinicians, prompting profound questions about its implications for health and disease. Among the contributing factors, one intriguing actor in this complex interplay is human cytomegalovirus (CMV), a member of the herpesvirus family. Latent CMV infection exerts a profound influence on the aging immune system, potentially contributing to age-related diseases. This review delves into the intricate relationship between immunosenescence and CMV, revealing how chronic viral infection impacts the aging immune landscape. We explore the mechanisms through which CMV can impact both the composition and functionality of immune cell populations and induce shifts in inflammatory profiles with aging. Moreover, we examine the potential role of CMV in pathologies such as cardiovascular diseases, cancer, neurodegenerative disorders, COVID-19, and Long COVID. This review underlines the importance of understanding the complex interplay between immunosenescence and CMV. It offers insights into the pathophysiology of aging and age-associated diseases, as well as COVID-19 outcomes among the elderly. By unraveling the connections between immunosenescence and CMV, we gain a deeper understanding of aging’s remarkable journey and the profound role that viral infections play in transforming the human immune system.
Furanocoumarins as Enhancers of Antitumor Potential of Sorafenib and LY294002 toward Human Glioma Cells In Vitro
Authors: Sumorek-Wiadro Joanna , Zając Adrian , Skalicka-Woźniak Krystyna , Rzeski Wojciech , Jakubowicz-Gil Joanna
Published: January 2024

Furanocoumarins are naturally occurring compounds in the plant world, characterized by low molecular weight, simple chemical structure, and high solubility in most organic solvents. Additionally, they have a broad spectrum of activity, and their properties depend on the location and type of attached substituents. Therefore, the aim of our study was to investigate the anticancer activity of furanocoumarins (imperatorin, isoimperatorin, bergapten, and xanthotoxin) in relation to human glioblastoma multiforme (T98G) and anaplastic astrocytoma (MOGGCCM) cell lines. The tested compounds were used for the first time in combination with LY294002 (PI3K inhibitor) and sorafenib (Raf inhibitor). Apoptosis, autophagy, and necrosis were identified microscopically after straining with Hoechst 33342, acridine orange, and propidium iodide, respectively. The levels of caspase 3 and Beclin 1 were estimated by immunoblotting and for the blocking of Raf and PI3K kinases, the transfection with specific siRNA was used. The scratch test was used to assess the migration potential of glioma cells. Our studies showed that the anticancer activity of furanocoumarins strictly depended on the presence, type, and location of substituents. The obtained results suggest that achieving higher pro-apoptotic activity is determined by the presence of an isoprenyl moiety at the C8 position of the coumarin skeleton. In both anaplastic astrocytoma and glioblastoma, imperatorin was the most effective in induction apoptosis. Furthermore, the usage of imperatorin, alone and in combination with sorafenib or LY294002, decreased the migratory potential of MOGGCCM and T98G cells.
Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review
Authors: Tominami Kanako , Kudo Tada-aki , Noguchi Takuya , Hayashi Yohei , Luo You-Ran , Tanaka Takakuni , Matsushita Ayumu , Izumi Satoshi , Sato Hajime , Gengyo-Ando Keiko , Matsuzawa Atsushi , Hong Guang , Nakai Junichi
Published: January 2024

PC12 cells, which are derived from rat adrenal pheochromocytoma cells, are widely used for the study of neuronal differentiation. NGF induces neuronal differentiation in PC12 cells by activating intracellular pathways via the TrkA receptor, which results in elongated neurites and neuron-like characteristics. Moreover, the differentiation requires both the ERK1/2 and p38 MAPK pathways. In addition to NGF, BMPs can also induce neuronal differentiation in PC12 cells. BMPs are part of the TGF-β cytokine superfamily and activate signaling pathways such as p38 MAPK and Smad. However, the brief lifespan of NGF and BMPs may limit their effectiveness in living organisms. Although PC12 cells are used to study the effects of various physical stimuli on neuronal differentiation, the development of new methods and an understanding of the molecular mechanisms are ongoing. In this comprehensive review, we discuss the induction of neuronal differentiation in PC12 cells without relying on NGF, which is already established for electrical, electromagnetic, and thermal stimulation but poses a challenge for mechanical, ultrasound, and light stimulation. Furthermore, the mechanisms underlying neuronal differentiation induced by physical stimuli remain largely unknown. Elucidating these mechanisms holds promise for developing new methods for neural regeneration and advancing neuroregenerative medical technologies using neural stem cells.
Hyperoxia: Effective Mechanism of Hyperbaric Treatment at Mild-Pressure
Authors: Cannellotto Mariana , Yasells García Ali , Landa María Silvina
Published: January 2024

HBOT increases the proportion of dissolved oxygen in the blood, generating hyperoxia. This increased oxygen diffuses into the mitochondria, which consume the majority of inhaled oxygen and constitute the epicenter of HBOT effects. In this way, the oxygen entering the mitochondria can reverse tissue hypoxia, activating the electron transport chain to generate energy. Furthermore, intermittent HBOT is sensed by the cell as relative hypoxia, inducing cellular responses such as the activation of the HIF-1α pathway, which in turn, activates numerous cellular processes, including angiogenesis and inflammation, among others. These effects are harnessed for the treatment of various pathologies. This review summarizes the evidence indicating that the use of medium-pressure HBOT generates hyperoxia and activates cellular pathways capable of producing the mentioned effects. The possibility of using medium-pressure HBOT as a direct or adjunctive treatment in different pathologies may yield benefits, potentially leading to transformative therapeutic advancements in the future.
A Deep Neural Network-Ensemble Adjustment Kalman Filter and Its Application on Strongly Coupled Data Assimilation
Authors: Wang Renxi , Shen Zheqi
Published: January 2024

This paper introduces a novel ensemble adjustment Kalman filter (EAKF) that integrates a machine-learning approach. The conventional EAKF adopts linear and Gaussian assumptions, making it difficult to handle cross-component updates in strongly coupled data assimilation (SCDA). The new approach employs nonlinear variable relationships established by a deep neural network (DNN) during the analysis stage of the EAKF, which nonlinearly projects observation increments into the state variable space. It can diminish errors in estimating cross-component error covariance arising from insufficient ensemble members, therefore improving the SCDA analysis. A conceptual coupled model is employed in this paper to conduct twin experiments, validating the DNN–EAKF’s capability to outperform conventional EAKF in SCDA. The results reveal that the DNN–EAKF can make SCDA superior to WCDA with a limited ensemble size. The root-mean-squared errors are reduced up to 70% while the anomaly correlation coefficients are increased up to 20% when the atmospheric observations are used to update the ocean variables directly. The other model components can also be improved through SCDA. This approach is anticipated to offer insights for future methodological integrations of machine learning and data assimilation and provide methods for SCDA applications in coupled general circulation models.
Quasi-Static Model Test of Pile-Supported Wharf under Cyclic Lateral Loading
Authors: Wang Jianfeng , Su Lei , Xie Libo , Ling Xianzhang
Published: January 2024

The pile-supported wharf (PSW) is one of the most common structures in harbor engineering. To investigate the dynamic response characteristics of the PSW, a quasi-static model test of a PSW–ground system under cyclic lateral loading is conducted. The deformation and damage processes of piles and the sand stratum are observed and obtained. The hysteresis characteristics of the PSW–ground system and the response characteristics of piles are systematically explored. The strain energy of the pile group on the sloping ground is calculated and analyzed. The results show that there are two peak bending moments on the pile, and the maximum one occurs at the pile top. The pile embedded depth and the loading direction strongly affect the strain energy distribution of the pile group. Under downslope direction loading, the center piles have the largest strain energy percentage. The strain energy extends from the center piles to the outside piles with the increase in loading displacement amplitude. The test results indicate that the landside pile and center pile should be strengthened during seismic design.
Carbon Intensity Assessment of a Bulk Carrier Operating in Different Sea State Conditions
Authors: Garbatov Yordan , Georgiev Petar
Published: January 2024

This work uses the environmental contour line approach to estimate the long-term extremes of carbon emission generated by a bulk carrier operating in different sea state conditions, utilising short-term analyses of the ship propulsion energy efficiency as a function of hull resistance in calm water due to appendages, aerodynamic resistance, and added wave resistance, resulting in the required permanent delivered power and the one induced by the waves. The analysis accounts for the ship’s main characteristics, operational profile based on mission conditions, and wave climatic data. All sources of inherent uncertainties are accounted for through the variability in the 3 h extreme value in any sea state in the long term, and the inverse first-order reliability method (IFORM) is employed in predicting the extreme operational carbon intensity indicator (CII). This study develops proper wave scatter diagrams as a function of the route description. The CII measures the energy efficiency of the installed propulsion system, accounting for the ship’s operational characteristics, such as the annual fuel consumption with corresponding CO2 factors, annual distance travelled, and capacity. The present study is limited to one operation route but can be extended to any other possible voyage or sea area. The estimated CII defined from the complete probabilistic characterisation of the sea state conditions conditional to the short-term maximum response is a rational approach that can be used for optimising the ship’s main characteristics, propulsion system, operational profile, and chosen route to achieve the best ship performance and energy efficiency.
Spatial Planning of Marine Protected Areas (MPAs) in the Southern Caspian Sea: Comparison of Multi-Criteria Evaluation (MCE) and Simulated Annealing Algorithm
Authors: Ashtab Dariush , Gholamalifard Mehdi , Jokar Parviz , Kostianoy Andrey G. , Semenov Aleksander V.
Published: January 2024

Protected areas are referred to around the world as the basis of conservation strategies. Designation of marine protected areas (MPAs) is to preserve marine biodiversity and protect species, habitats in the seas, and oceans. The simulated annealing algorithm (SAA) with other algorithms (swap iterative improvement, normal followed by two step, two step iterative improvement, and normal iterative improvement) in MARXAN conservation solutions software and the multi-criteria evaluation (MCE) method were used to locate MPAs in the Southern Caspian Sea. Then, four methods were examined for site selection that include: (1) Simulated annealing algorithm, (2) MCE with zonal land suitability (ZLS), (3) MCE with compactness and contiguity, and (4) combined method of multi-criteria evaluation with spatial constraints and a simulated annealing algorithm (improved MCE). In the MCE method, we applied different weighted scenarios to locate MPAs. The criteria for determining the desired regions of MPAs included 12 factors gathered in three groups, including: (1) Ecological criteria (distribution of fish Huso huso, Acipenser persicus, Acipenser stellatus, Rutilus frisii kutum, and Alosa braschnikowi; location of coastal protected areas, distance from coastal rivers (Coastline), distance from estuaries and deltas); (2) Physical criteria (distance from the coast, shore sensitive areas); and (3) Socio-economic criteria (distance from densely populated coastal cities, distance from industries near the coast). The results of comparing the algorithms in MARXAN 4.0.6 software showed that the simulated annealing algorithm has a better ratio of border-length/area than other algorithms. Also, the combined method of MCE (improved MCE) selects the best protection patches in terms of location, taking into account the seascape ecology metrics (e.g., patch compactness, edge density, normalized entropy, area metric for patches). Moreover, the results of the comparison of four methods for proposing MPAs based on seascape metrics showed that the combined method of MCE considers a protection network with more contiguity and compactness than the simulated annealing algorithm. The use of seascape ecology can help to preserve and create larger and denser patches in the arrangement of protective areas, because such a selection of protective areas is nature-inspired and can be more bold and appropriate in the course of conservation planning.
COVID-19 Crisis in Brazil: Post-Vaccination Seroprevalence and Infection in More and Less Vaccinated Municipalities in a Northeastern State
Authors: Santos Ronaldy Santana , Barreto Marina dos Santos , Silva Eloia Emanuelly Dias , Silva Beatriz Soares da , Jesus Pamela Chaves de , Silva Deise Maria Rego Rodrigues , Moura Pedro Henrique Macedo , Souza Jessiane Bispo de , Santos Leticia Milena Machado dos , Guimarães Adriana Gibara , Santana Lucas Alves da Mota , Borges Lysandro Pinto
Published: January 2024

Background: Given the impact of the pandemic in Brazil, vaccination is essential to prevent illness and death. Thus, this study sought to compare, after vaccination, the circulation of SARS-CoV-2 and the response to vaccination in the least and most vaccinated municipalities of a Brazilian state during the height of the pandemic when the Omicron variant was dominant. Methods: We tested for the SARS-CoV-2 antigen and confirmed infection using an RT-qPCR and measured IgM and IgG antibodies in fully vaccinated participants from municipalities with higher and lower vaccination rates. Results: We showed that participants from the least vaccinated municipalities were more likely to have detectable IgM antibodies and a positive antigen/RT-qPCR result for SARS-CoV-2 than participants from the most vaccinated municipalities. There were no differences between the vaccines used (BNT162b2, Ad26.COV2.S, AZD1222, and CoronaVac) and antibody production. Conclusions: Our study evaluated municipal vaccination coverage and its effects on mortality, infections, and anti-SARS-CoV-2 antibodies during a critical phase of the pandemic. The results suggest that higher vaccination coverage reduces acute cases and confers higher memory antibody levels against SARS-CoV-2. Even with a full vaccination schedule, individuals living in places with low vaccination rates are more susceptible to infection.
The Most Recent Insights into the Roots of Gastric Cancer
Authors: Meliț Lorena Elena , Mărginean Cristina Oana , Borka Balas Reka
Published: January 2024

Helicobacter pylori (H. pylori) is the most common bacterial infection worldwide, usually being acquired during childhood, and its persistence into adulthood represents one of the main contributors of gastric carcinogenesis. Based on these statements, it would be of great importance to know if the most early premalignant transformation occurs in children or later since, this would enable the development of effective anti-tumorigenesis strategies. The interplay between H. pylori virulence factors, the host’s responses modified by this infection, and the gastric microecology are complex and eventually lead to the development of gastric cancer in susceptible individuals. Several biomarkers were identified as major contributors of this long-lasting process, such as pepsinogens, gastrin 17, lipid-, glucose- and iron-metabolism parameters, immunity players, aberrant bacterial DNA methylation, H. pylori virulence factors, and hallmarks of gastric dysbiosis. Several of these biomarkers were also identified in children with H. pylori infection, independently of the presence of premalignant lesions, which were also proven to be present in a subgroup of H. pylori-infected children, especially those carrying extremely virulent strains. Therefore, the most incipient premalignant gastric changes might indeed occur early during childhood, opening a promising research gate for further studies to delineate the border between infection and cancer.