bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolic Effects of Glucose-Fructose Co-Ingestion Compared to Glucose Alone during Exercise in Type 1 Diabetes
Authors: Bally Lia , Kempf Patrick , Zueger Thomas , Speck Christian , Pasi Nicola , Ciller Carlos , Feller Katrin , Loher Hannah , Rosset Robin , Wilhelm Matthias , Boesch Chris , Buehler Tania , Dokumaci Ayse , Tappy Luc , Stettler Christoph
Published: February 2017

This paper aims to compare the metabolic effects of glucose-fructose co-ingestion (GLUFRU) with glucose alone (GLU) in exercising individuals with type 1 diabetes mellitus. Fifteen male individuals with type 1 diabetes (HbA1c 7.0% ± 0.6% (53 ± 7 mmol/mol)) underwent a 90 min iso-energetic continuous cycling session at 50% VO2max while ingesting combined glucose-fructose (GLUFRU) or glucose alone (GLU) to maintain stable glycaemia without insulin adjustment. GLUFRU and GLU were labelled with 13C-fructose and 13C-glucose, respectively. Metabolic assessments included measurements of hormones and metabolites, substrate oxidation, and stable isotopes. Exogenous carbohydrate requirements to maintain stable glycaemia were comparable between GLUFRU and GLU (p = 0.46). Fat oxidation was significantly higher (5.2 ± 0.2 vs. 2.6 ± 1.2 mg·kg−1·min−1, p < 0.001) and carbohydrate oxidation lower (18.1 ± 0.8 vs. 24.5 ± 0.8 mg·kg−1·min−1 p < 0.001) in GLUFRU compared to GLU, with decreased muscle glycogen oxidation in GLUFRU (10.2 ± 0.9 vs. 17.5 ± 1.0 mg·kg−1·min−1, p < 0.001). Lactate levels were higher (2.2 ± 0.2 vs. 1.8 ± 0.1 mmol/L, p = 0.012) in GLUFRU, with comparable counter-regulatory hormones between GLUFRU and GLU (p > 0.05 for all). Glucose and insulin levels, and total glucose appearance and disappearance were comparable between interventions. Glucose-fructose co-ingestion may have a beneficial impact on fuel metabolism in exercising individuals with type 1 diabetes without insulin adjustment, by increasing fat oxidation whilst sparing glycogen.
The Effects of Moderate Whole Grain Consumption on Fasting Glucose and Lipids, Gastrointestinal Symptoms, and Microbiota
Authors: Cooper Danielle , Kable Mary , Marco Maria , De Leon Angela , Rust Bret , Baker Julita , Horn William , Burnett Dustin , Keim Nancy
Published: February 2017

This study was designed to determine if providing wheat, corn, and rice as whole (WG) or refined grains (RG) under free-living conditions will change parameters of health over a six-week intervention in healthy, habitual non-WG consumers. Measurements of body composition, fecal microbiota, fasting blood glucose, total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), and triglycerides were made at baseline and post intervention. Subjects were given adequate servings of either WG or RG products based on their caloric need and asked to keep records of grain consumption, bowel movements, and GI symptoms weekly. After six weeks, subjects repeated baseline testing. Significant decreases in total, LDL, and non-HDL cholesterol were seen after the WG treatments but were not observed in the RG treatment. During Week 6, bowel movement frequency increased with increased WG consumption. No significant differences in microbiota were seen between baseline and post intervention, although, abundance of order Erysipelotrichales increased in RG subjects who ate more than 50% of the RG market basket products. Increasing consumption of WGs can alter parameters of health, but more research is needed to better elucidate the relationship between the amount consumed and the health-related outcome.
A Village‐Based Intervention: Promoting Folic Acid Use among Rural Chinese Women
Authors: Lin Qian , Yang Lina , Li Fang , Qin Hong , Li Mingzhi , Chen Jihua , Deng Jing , Hu Xiangying
Published: February 2017

Background: Folic acid supplementation is effective in reducing the risk of neural tube defects (NTDs). However, the use of folic acid is low among rural women in China. Nutrition education can provide information about folic acid and encourage its use. The primary objective of this study was to test the effectiveness of a village‐based nutrition intervention on folic acid use among rural women. Methods: Sixty villages were randomly selected using multiple‐stage sampling and were divided into control and intervention groups. The intervention included nutritional education at village clinics, written materials, and text messages (SMS). Folic acid use knowledge and behavior was assessed at baseline and after the intervention. Results: Self‐reported compliance with folic acid supplement use increased from 17.0%–29.2% at baseline to 41.7%-59.2% one year post‐intervention. During the same period, the folic acid knowledge score in the intervention group increased from 3.07 to 3.65, significantly higher than the control group (3.11 to 3.35). Multivariate binary logistic regression showed that the women who received folic acid education and SMS intervention were more likely to comply with folic acid supplement recommendations. Conclusions: The results indicated that an integrated village‐based folic acid education intervention may be an effective way of promoting folic acid use for the prevention of NTDs in rural women.
Age-Related Loss in Bone Mineral Density of Rats Fed Lifelong on a Fish Oil-Based Diet Is Avoided by Coenzyme Q10 Addition
Authors: Varela-López Alfonso , Ochoa Julio , Llamas-Elvira José , López-Frías Magdalena , Planells Elena , Ramirez-Tortosa MCarmen , Ramirez-Tortosa Cesar , Giampieri Francesca , Battino Maurizio , Quiles José
Published: February 2017

During aging, bone mass declines increasing osteoporosis and fracture risks. Oxidative stress has been related to this bone loss, making dietary compounds with antioxidant properties a promising weapon. Male Wistar rats were maintained for 6 or 24 months on diets with fish oil as unique fat source, supplemented or not with coenzyme Q10 (CoQ10), to evaluate the potential of adding this molecule to the n-3 polyunsaturated fatty acid (n-3 PUFA)-based diet for bone mineral density (BMD) preservation. BMD was evaluated in the femur. Serum osteocalcin, osteopontin, receptor activator of nuclear factor-κB ligand, ostroprotegerin, parathyroid hormone, urinary F2-isoprostanes, and lymphocytes DNA strand breaks were also measured. BMD was lower in aged rats fed a diet without CoQ10 respect than their younger counterparts, whereas older animals receiving CoQ10 showed the highest BMD. F2-isoprostanes and DNA strand breaks showed that oxidative stress was higher during aging. Supplementation with CoQ10 prevented oxidative damage to lipid and DNA, in young and old animals, respectively. Reduced oxidative stress associated to CoQ10 supplementation of this n-3 PUFA-rich diet might explain the higher BMD found in aged rats in this group of animals.
Polypharmacy in Zoological Medicine
Authors: Hunter Robert , Isaza Ramiro
Published: February 2017

Polypharmacy is a term that describes the inappropriate, concurrent use of multiple drugs in an individual patient. Zoological medicine practitioners must take approved agents (veterinary or human) and extrapolate their use to non-approved species often with little species-specific pharmacological evidence to support their decisions. When considering polypharmacy, even less information exists concerning multi-drug pharmacokinetics, pharmacodynamics, or potential drug-drug interactions in non-domestic species. Unfortunately, captive, zoological species are susceptible, just like their domestic counterparts, to chronic diseases and co-morbidities that may lead to the usage of multiple drugs. Polypharmacy is a recognized and important issue in human medicine, as well as an emerging issue for veterinarians; thus, this paper will discuss the novel, potential risks of polypharmacy in zoological medicine. Hopefully, this discussion will help bring the attention of veterinarians to this issue and serve as an interesting discussion topic for pharmacologists in general.
Tailoring Copolymer Properties by Gradual Changes in the Distribution of the Chains Composition Using Semicontinuous Emulsion Polymerization
Authors: Jasso-Gastinel Carlos , Arnez-Prado Alvaro , Aranda-García Francisco , Sahagún-Aguilar Luis , López-Dellamary Toral Fernando , Hernández-Hernández María , González-Ortiz Luis
Published: February 2017

To design the properties of a copolymer using free radical polymerization, a semicontinuous process can be applied to vary the instantaneous copolymer composition along the conversion searching for a specific composition spectrum of copolymer chains, which can be termed as weight composition distribution (WCD) of copolymer chains. Here, the styrene-n-butyl acrylate (S/BA) system was polymerized by means of a semicontinuous emulsion process, varying the composition of the comonomer feed to obtain forced composition copolymers (FCCs). Five different feeding profiles were used, searching for a scheme to obtain chains rich in S (looking for considerable modulus), and chains rich in BA (looking for large deformation) as a technique to achieve synergy in copolymer properties; the mechanostatic and dynamic characterization discloses the correspondence between WCD and the bulk properties. 1H-nuclear magnetic resonance (1H-NMR) analysis enabled the determination of the cumulative copolymer composition characterization, required to estimate the WCD. The static test (stress-strain) and dynamic mechanical analysis (DMA) were performed following normed procedures. This is the first report that shows very diverse mechanostatic performances of copolymers obtained using the same chemical system and global comonomer composition, forming a comprehensive failure envelope, even though the tests were carried out at the same temperature and cross head speed. The principles for synergic performance can be applied to controlled radical copolymerization, designing the composition variation in individual molecules along the conversion.
Bond Performance of Sand Coated UHM CFRP Tendons in High Performance Concrete
Authors: Lämmlein Tobias , Messina Francesco , Griffa Michele , Terrasi Giovanni , Lura Pietro
Published: February 2017

The bond behaviour of novel, sand-coated ultra-high modulus (UHM) carbon fibre reinforced polymers (CFRP) tendons to high performance concrete (HPC) was studied by a combined numerical and experimental approach. A series of pull-out tests revealed that the failure type can vary between sudden and continuous pull-out depending on the chosen sand coating grain size. Measuring the same shear stress vs. tendon draw-in (τ-δ) curves in the same test set-up, for sand coated CFRP tendons with a longitudinal stiffness of 137 and 509 GPa, respectively, indicated that the absolute bond strength in both cases was not influenced by the tendon’s stiffness. However, the τ-δ curves significantly differed in terms of the draw-in rate, showing higher draw-in rate for the UHM CFRP tendon. With the aid of X-ray computed tomography (CT), scanning electron microscopy (SEM) and visual analysis methods, the bond failure interface was located between the CFRP tendon and the surrounding sand-epoxy layer. For further investigation, a simplified finite element analysis (FEA) of the tendon pull-out was performed using a cohesive surface interaction model and the software Abaqus 6.14. A parametric study, varying the tendon-related material properties, revealed the tendon’s longitudinal stiffness to be the only contributor to the difference in the τ-δ curves found in the experiments, thus to the shear stress transfer behaviour between the CFRP tendon and the concrete. In conclusion, the excellent bond of the sand-coated UHM CFRP tendons to HPC as well as the deeper insight in the bond failure mechanism encourages the application of UHM CFRP tendons for prestressing applications.
Poly(methacrylic acid-ran-2-vinylpyridine) Statistical Copolymer and Derived Dual pH-Temperature Responsive Block Copolymers by Nitroxide-Mediated Polymerization
Authors: Marić Milan , Zhang Chi , Gromadzki Daniel
Published: February 2017

Nitroxide-mediated polymerization using the succinimidyl ester functional unimolecular alkoxyamine initiator (NHS-BlocBuilder) was used to first copolymerize tert-butyl methacrylate/2-vinylpyridine (tBMA/2VP) with low dispersity (Đ = 1.30–1.41) and controlled growth (linear number average molecular Mn versus conversion, Mn = 3.8–10.4 kg·mol−1) across a wide composition of ranges (initial mol fraction 2VP, f2VP,0 = 0.10–0.90). The resulting statistical copolymers were first de-protected to give statistical polyampholytic copolymers comprised of methacrylic acid/2VP (MAA/2VP) units. These copolymers exhibited tunable water-solubility due to the different pKas of the acidic MAA and basic 2VP units; being soluble at very low pH < 3 and high pH > 8. One of the tBMA/2VP copolymers was used as a macroinitiator for a 4-acryloylmorpholine/4-acryloylpiperidine (4AM/4AP) mixture, to provide a second block with thermo-responsive behavior with tunable cloud point temperature (CPT), depending on the ratio of 4AM:4AP. Dynamic light scattering of the block copolymer at various pHs (3, 7 and 10) as a function of temperature indicated a rapid increase in particle size >2000 nm at 22–27 °C, corresponding to the 4AM/4AP segment’s thermos-responsiveness followed by a leveling in particle size to about 500 nm at higher temperatures.
Reformation Leads to Self-Reliance: The Protestantism of Transcendentalism
Authors: Griffis Rachel
Published: February 2017

This article examines connections between the Protestant Reformation and American literature and argues that Protestantism’s best expression exists in contemporary iterations of self-reliance. The first part focuses on William Ellery Channing’s and Ralph Waldo Emerson’s literary criticism of John Milton, a poet who represents the Protestant ideals these writers combine with American principles to develop the literary tradition. The second part discusses the trajectory of American literature in the nineteenth century and extends this discussion to current assumptions regarding teaching and learning.
A Radiometric Uncertainty Tool for the Sentinel 2 Mission
Authors: Gorroño Javier , Fomferra Norman , Peters Marco , Gascon Ferran , Underwood Craig , Fox Nigel , Kirches Grit , Brockmann Carsten
Published: February 2017

In the framework of the European Copernicus programme, the European Space Agency (ESA) has launched the Sentinel-2 (S2) Earth Observation (EO) mission which provides optical high spatial resolution imagery over land and coastal areas. As part of this mission, a tool (named S2-RUT, from Sentinel-2 Radiometric Uncertainty Tool) has been developed. The tool estimates the radiometric uncertainty associated with each pixel in the top-of-atmosphere (TOA) reflectance factor images provided by ESA. This paper describes the design and development process of the initial version of the S2-RUT tool. The initial design step describes the S2 radiometric model where a set of uncertainty contributors are identified. Each of the uncertainty contributors is specified by reviewing the pre- and post-launch characterisation. The identified uncertainty contributors are combined following the guidelines in the ‘Guide to Expression of Uncertainty in Measurement’ (GUM) model and this combination model is further validated by comparing the results to a multivariate Monte Carlo Method (MCM). In addition, the correlation between the different uncertainty contributions and the impact of simplifications in the combination model have been studied. The software design of the tool prioritises an efficient strategy to read the TOA reflectance factor images, extract the auxiliary information from the metadata in the satellite products and the codification of the resulting uncertainty image. This initial version of the tool has been implemented and integrated as part of the Sentinels Application Platform (SNAP).
Post-Injury and Resolution Response to Repetitive Inhalation Exposure to Agricultural Organic Dust in Mice
Authors: Warren Kristi , Wyatt Todd , Romberger Debra , Ailts Isaak , West William , Nelson Amy , Nordgren Tara , Staab Elizabeth , Heires Art , Poole Jill
Published: February 2017

Inhalation of organic dusts in agricultural environments causes airway inflammatory diseases. Despite advances in understanding the airway response to dust-induced inflammation, less is known about the transition from lung injury to repair and recovery. The objective of this study was to define the post-inflammation homeostasis events following organic dust-induced lung injury. Using an established protocol, mice were intranasally treated with swine confinement facility organic dust extract (ODE) daily for 3 weeks (repetitive exposure) or treated daily with ODE for 3 weeks followed by no treatment for 1-4 weeks (recovery period) whereupon lavage fluid, lung tissue, and sera were processed. During recovery period, a significant decrease was observed in ODE-induced neutrophil levels after 1 week, lymphocytes at 2 weeks, and macrophages at 4 weeks in the lavage fluid. ODE-induced lung cellular aggregates and bronchiolar compartment inflammation were diminished, but persisted for 4 weeks post-injury. Alveolar inflammation resolved at 3 weeks. ODE-induced lung neutrophils were cleared by 3 weeks, B-cells by 2 weeks, and CD3+CD4+ and CD3+CD8+ T cells by 4 week recovery period. Amphiregulin levels increased post-ODE exposure to the 4 week recovery period. Ex vivo amphiregulin production was demonstrated in lung type 2 innate lymphoid cells and macrophages isolated from the 4 week recovery (post-ODE exposure) animals. Collectively, these results identify important processes during recovery period following agricultural dust-induced inflammation, and present possible strategies for improving lung repair and resolution.
The Emergency Vehicle Routing Problem with Uncertain Demand under Sustainability Environments
Authors: Qin Jin , Ye Yong , Cheng Bi-rong , Zhao Xiaobo , Ni Linling
Published: February 2017

The reasonable utilization of limited resources is critical to realize the sustainable developments. In the initial 72-h crucial rescue period after the disaster, emergency supplies have always been insufficient and the demands in the affected area have always been uncertain. In order to improve timeliness, utilization and sustainability of emergency service, the allocation of the emergency supplies and the emergency vehicle routes should be determined simultaneously. Assuming the uncertain demands follow normal distribution, an optimization model for the emergency vehicle routing, by considering the insufficient supplies and the uncertain demands, is developed. The objective function is applied to minimize the total costs, including the penalty costs induced by more or less supplies than the actual demands at all demand points, as well as the constraints of the time windows and vehicle load capacity taken into account. In more details, a solution method for the model, based on the genetic algorithm, is proposed, which solves the problem in two stages. A numerical example is presented to demonstrate the efficiency and validity of the proposed model and algorithm.
Weight Determination of Sustainable Development Indicators Using a Global Sensitivity Analysis Method
Authors: Luan Wenfei , Lu Ling , Li Xin , Ma Chunfeng
Published: February 2017

Sustainable development (SD) evaluations have attracted considerable attention from governments and scientific communities around the world. The objective and quantitative calculation of the importance of sustainable assessment indicators is a key problem in the accurate evaluation of SD. Traditional methods fail to quantify the coupling effects among indicators. This paper presents a weight determination approach based on the global sensitivity analysis algorithm known as the extended Fourier amplitude sensitivity test (EFAST). This method is efficient and robust and is not only able to quantify the sensitivity of the evaluation indictors to the target, but can also quantitatively describe the uncertainties among the indictors. In this paper, we analyze the sensitivity of 18 indicators in a multi-index comprehensive evaluation model and weigh the indicators in the system according to their importance. To verify the feasibility and advantages of this new method, we compare the evaluation result with the traditional entropy method. The comparison shows that the EFAST algorithm can provide greater detail in an SD evaluation. Additionally, the EFAST algorithm is more specific in terms of quantitative analysis and comprehensive aspects and can more effectively distinguish the importance of indicators.
Balancing Disassembly Line in Product Recovery to Promote the Coordinated Development of Economy and Environment
Authors: Liu Jia , Wang Shuwei
Published: February 2017

For environmentally conscious and sustainable manufacturing, many more manufacturers are acting to recycle and remanufacture their post-consumed products. The most critical process of remanufacturing is disassembly, since it allows for the selective extraction of the valuable components and materials from returned products to reduce the waste disposal volume. It is, therefore, important to design and balance the disassembly line to work efficiently due to its vital role in effective resource usage and environmental protection. Considering the disassembly precedence relationships and sequence-dependent parts removal time increments, this paper presents an improved discrete artificial bee colony algorithm (DABC) for solving the sequence-dependent disassembly line balancing problem (SDDLBP). The performance of the proposed algorithm was tested against nine other approaches. Computational results evidently indicate the superior efficiency of the proposed algorithm for addressing the environmental and economic concerns while optimizing the multi-objective SDDLBP.
Effectiveness Evaluation for a Commercialized PV-Assisted Charging Station
Authors: Liu Nian , Cheng Minyang
Published: February 2017

The Photovoltaic–assisted Charging Station (PVCS) is regarded as one of the most promising charging facilities for future electric vehicle (EV) energy supplementation. In this paper, the operation mode and profitability of a commercialized PVCS are analyzed under the energy policy of China. In order to evaluate the long-term effectiveness of using the PVCS to provide guidance for the investors of the stations, a set of evaluation indexes is introduced, including the quality of service, the environmental and economic benefits, and the impacts on the grid. Furthermore, an easily-achieved charging strategy which considers the quality of service and the self-consumption of PV energy is proposed. Finally, an effectiveness evaluation for different operational scenarios of the PVCS is completed, based on the actual statistical data. The simulation and evaluation results indicate that the PVCS has the potential to produce satisfactory environmental/economic benefits and to reduce the impacts and dependence of an EV’s charging load on the grid.
Symmetry Analysis and Conservation Laws of the Zoomeron Equation
Authors: Motsepa Tanki , Khalique Chaudry , Gandarias Maria
Published: February 2017

In this work, we study the (2 + 1)-dimensional Zoomeron equation which is an extension of the famous (1 + 1)-dimensional Zoomeron equation that has been studied extensively in the literature. Using classical Lie point symmetries admitted by the equation, for the ﬁrst time we develop an optimal system of one-dimensional subalgebras. Based on this optimal system, we obtain symmetry reductions and new group-invariant solutions. Again for the ﬁrst time, we construct the conservation laws of the underlying equation using the multiplier method.
Formal Proof of the Dependable Bypassing Routing Algorithm Suitable for Adaptive Networks on Chip QnoC Architecture
Authors: Daoud Hayat , Tanougast Camel , Belarbi Mostefa , Heil Mikael , Diou Camille
Published: February 2017

Approaches for the design of fault tolerant Network-on-Chip (NoC) for use in System-on-Chip (SoC) reconfigurable technology using Field-Programmable Gate Array (FPGA) technology are challenging, especially in Multiprocessor System-on-Chip (MPSoC) design. To achieve this, the use of rigorous formal approaches, based on incremental design and proof theory, has become an essential step in the validation process. The Event-B method is a promising formal approach that can be used to develop, model and prove accurately SoC and MPSoC architectures. This paper proposes a formal verification approach for NoC architecture including the dependability constraints relating to the choice of the path routing of data packets and the strategy imposed for diversion when faulty routers are detected. The formalization process is incremental and validated by correct-by-construction development of the NoC architecture. Using the concepts of graph colouring and B-event formalism, the results obtained have demonstrated its efficiency for determining the bugs, and a solution to ensure a fast and reliable operation of the network when compared to existing similar methods.
Fungal Ribotoxins: A Review of Potential Biotechnological Applications
Authors: Olombrada Miriam , Lázaro-Gorines Rodrigo , López-Rodríguez Juan , Martínez-del-Pozo Álvaro , Oñaderra Mercedes , Maestro-López Moisés , Lacadena Javier , Gavilanes José , García-Ortega Lucía
Published: February 2017

Fungi establish a complex network of biological interactions with other organisms in nature. In many cases, these involve the production of toxins for survival or colonization purposes. Among these toxins, ribotoxins stand out as promising candidates for their use in biotechnological applications. They constitute a group of highly specific extracellular ribonucleases that target a universally conserved sequence of RNA in the ribosome, the sarcin-ricin loop. The detailed molecular study of this family of toxic proteins over the past decades has highlighted their potential in applied research. Remarkable examples would be the recent studies in the field of cancer research with promising results involving ribotoxin-based immunotoxins. On the other hand, some ribotoxin-producer fungi have already been studied in the control of insect pests. The recent role of ribotoxins as insecticides could allow their employment in formulas and even as baculovirus-based biopesticides. Moreover, considering the important role of their target in the ribosome, they can be used as tools to study how ribosome biogenesis is regulated and, eventually, may contribute to a better understanding of some ribosomopathies.
Bat Astroviruses: Towards Understanding the Transmission Dynamics of a Neglected Virus Family
Authors: Fischer Kerstin , Pinho dos Reis Vinícius , Balkema-Buschmann Anne
Published: February 2017

Bats belong to the order Chiroptera that represents the second largest order of mammals with more than 1200 species and an almost global distribution. Environmental changes and deforestation have severely influenced many ecosystems, intensifying the contact between wildlife and humans. In recent years, bats have been found to harbor a number of different viruses with zoonotic potential, as well as a great diversity of astroviruses, for which the question of zoonotic potential remains unanswered to date. Human astroviruses have been identified as the causative agent for diarrhea in children and immunocompromised patients. For a long time, astroviruses have been considered to be strictly species-specific. However, a great genetic diversity has recently been discovered among animal and human astroviruses that might indicate the potential of these viruses to cross species barriers. Furthermore, our knowledge about the tissue tropism of astroviruses has been expanded to some neurotropic strains that have recently been shown to be responsible for encephalitis in humans and livestock. This review gives an overview on what is known about astroviruses in bats, humans and livestock, especially bovines and pigs. Future research activities are suggested to unravel astrovirus infection dynamics in bat populations to further assess the zoonotic potential of these viruses.
PreC and C Regions of Woodchuck Hepatitis Virus Facilitate Persistent Expression of Surface Antigen of Chimeric WHV-HBV Virus in the Hydrodynamic Injection BALB/c Mouse Model
Authors: Wu Weimin , Liu Yan , Lin Yong , Pan Danzhen , Yang Dongliang , Lu Mengji , Xu Yang
Published: February 2017

In the hydrodynamic injection (HI) BALB/c mouse model with the overlength viral genome, we have found that woodchuck hepatitis virus (WHV) could persist for a prolonged period of time (up to 45 weeks), while hepatitis B virus (HBV) was mostly cleared at week four. In this study, we constructed a series of chimeric genomes based on HBV and WHV, in which the individual sequences of a 1.3-fold overlength HBV genome in pBS-HBV1.3 were replaced by their counterparts from WHV. After HI with the WHV-HBV chimeric constructs in BALB/c mice, serum viral antigen, viral DNA (vDNA), and intrahepatic viral antigen expression were analyzed to evaluate the persistence of the chimeric genomes. Interestingly, we found that HI with three chimeric WHV-HBV genomes resulted in persistent antigenemia in mice. All of the persistent chimeric genomes contained the preC region and the part of the C region encoding the N-terminal 1–145 amino acids of the WHV genome. These results indicated that the preC region and the N-terminal part of the C region of the WHV genome may play a role in the persistent antigenemia. The chimeric WHV-HBV genomes were able to stably express viral antigens in the liver and could be further used to express hepadnaviral antigens to study their pathogenic potential.
Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis
Authors: Wang Zhenchao , Yu Lu , Jin Linhong , Wang Wenli , Zhao Qi , Ran Longlu , Li Xiangyang , Chen Zhuo , Guo Rong , Wei Yongtian , Yang Zhongcheng , Liu Enlong , Hu Deyu , Song Baoan
Published: February 2017

Diseases caused by southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) considerably decrease grain yield. Therefore, determining rice cultivars with high resistance to SRBSDV and RRSV is necessary. In this study, rice cultivars with high resistance to SRBSDV and RRSV were evaluated through field trials in Shidian and Mangshi county, Yunnan province, China. SYBR Green I-based quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to quantitatively detect virus gene expression levels in different rice varieties. The following parameters were applied to evaluate rice resistance: acre yield (A.Y.), incidence of infected plants (I.I.P.), virus load (V.L.), disease index (D.I.), and insect quantity (I.Q.) per 100 clusters. Zhongzheyou1 (Z1) and Liangyou2186 (L2186) were considered the most suitable varieties with integrated higher A.Y., lower I.I.P., V.L., D.I. and I.Q. features. In order to investigate the mechanism of rice resistance, comparative label-free shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomic approaches were applied to comprehensively describe the proteomics of rice varieties’ SRBSDV tolerance. Systemic acquired resistance (SAR)-related proteins in Z1 and L2186 may result in the superior resistance of these varieties compared with Fengyouxiangzhan (FYXZ).
Automated Extraction of Urban Water Bodies from ZY‐3 Multi‐Spectral Imagery
Authors: Yang Fan , Guo Jianhua , Tan Hai , Wang Jingxue
Published: February 2017

The extraction of urban water bodies from high‐resolution remote sensing images, which has been a hotspot in researches, has drawn a lot of attention both domestic and abroad. A challenging issue is to distinguish the shadow of high‐rise buildings from water bodies. To tackle this issue, we propose the automatic urban water extraction method (AUWEM) to extract urban water bodies from high‐resolution remote sensing images. First, in order to improve the extraction accuracy, we refine the NDWI algorithm. Instead of Band2 in NDWI, we select the first principal component after PCA transformation as well as Band1 for ZY‐3 multi‐spectral image data to construct two new indices, namely NNDWI1, which is sensitive to turbid water, and NNDWI2, which is sensitive to the water body whose spectral information is interfered by vegetation. We superimpose the image threshold segmentation results generated by applying NNDWI1 and NNDWI2, then detect and remove the shadows in the small areas of the segmentation results using object‐oriented shadow detection technology, and finally obtain the results of the urban water extraction. By comparing the Maximum Likelihood Method (MaxLike) and NDWI, we find that the average Kappa coefficients of AUWEM, NDWI and MaxLike in the five experimental areas are about 93%, 86.2% and 88.6%, respectively. AUWEM exhibits lower omission error rates and commission error rates compared with the NDWI and MaxLike. The average total error rates of the three methods are about 11.9%, 18.2%, and 22.1%, respectively. AUWEM not only shows higher water edge detection accuracy, but it also is relatively stable with the change of threshold. Therefore, it can satisfy demands of extracting water bodies from ZY‐3 images.
Developing Intensity–Duration–Frequency (IDF) Curves under Climate Change Uncertainty: The Case of Bangkok, Thailand
Authors: Shrestha Ashish , Babel Mukand , Weesakul Sutat , Vojinovic Zoran
Published: February 2017

The magnitude and frequency of hydrological events are expected to increase in coming years due to climate change in megacities of Asia. Intensity–Duration–Frequency (IDF) curves represent essential means to study effects on the performance of drainage systems. Therefore, the need for updating IDF curves comes from the necessity to gain better understanding of climate change effects. The present paper explores an approach based on spatial downscaling-temporal disaggregation method (DDM) to develop future IDFs using stochastic weather generator, Long Ashton Research Station Weather Generator (LARS-WG) and the rainfall disaggregation tool, Hyetos. The work was carried out for the case of Bangkok, Thailand. The application of LARS-WG to project extreme rainfalls showed promising results and nine global climate models (GCMs) were used to estimate changes in IDF characteristics for future time periods of 2011–2030 and 2046–2065 under climate change scenarios. The IDFs derived from this approach were corrected using higher order equation to mitigate biases. IDFs from all GCMs showed increasing intensities in the future for all return periods. The work presented demonstrates the potential of this approach in projecting future climate scenarios for urban catchment where long term hourly rainfall data are not readily available.
Modeling Rainfall-Runoff Response to Land Use and Land Cover Change in Rwanda (1990–2016)
Authors: Karamage Fidele , Zhang Chi , Fang Xia , Liu Tong , Ndayisaba Felix , Nahayo Lamek , Kayiranga Alphonse , Nsengiyumva Jean
Published: February 2017

Stormwater runoff poses serious environmental problems and public health issues in Rwanda, a tropical country that is increasingly suffering from severe floods, landslides, soil erosion and water pollution. Using the WetSpa Extension model, this study assessed the changes in rainfall runoff depth in Rwanda from 1990 to 2016 in response to precipitation and land use changes. Our results show that Rwanda has experienced a significant conversion of natural forest and grassland to cropland and built-up areas. During the period 1990–2016, 7090.02 km2 (64.5%) and 1715.26 km2 (32.1%) of forest and grassland covers were lost, respectively, while the cropland and built-up areas increased by 135.3% (8503.75 km2) and 304.3% (355.02 km2), respectively. According to our estimates, the land use change effect resulted in a national mean runoff depth increase of 2.33 mm/year (0.38%). Although precipitation change affected the inter-annual fluctuation of runoff, the long-term trend of runoff was dominated by land use change. The top five districts that experienced the annual runoff depth increase (all >3.8 mm/year) are Rubavu, Nyabihu, Ngororero, Gakenke, and Musanze. Their annual runoff depths increased at a rate of >3.8 mm/year during the past 27 years, due to severe deforestation (ranging from 62% to 85%) and cropland expansion (ranging from 123% to 293%). These areas require high priority in runoff control using terracing in croplands and rainwater harvesting systems such as dam/reservoirs, percolation tanks, storage tanks, etc. The wet season runoff was three times higher than the dry season runoff in Rwanda; appropriate rainwater management and reservation could provide valuable irrigation water for the dry season or drought years (late rainfall onsets or early rainfall cessations). It was estimated that a reservation of 30.5% (3.99 km3) of the runoff in the wet season could meet the cropland irrigation water gap during the dry season in 2016.
Improvements to Runoff Predictions from a Land Surface Model with a Lateral Flow Scheme Using Remote Sensing and In Situ Observations
Authors: Lee Jong , Choi Hyun
Published: February 2017

Like most land surface models (LSMs) coupled to regional climate models (RCMs), the original Common Land Model (CoLM) predicts runoff from net water at each computational grid without explicit lateral flow (LF) schemes. This study has therefore proposed a CoLM+LF model incorporating a set of lateral surface and subsurface runoff computations controlled by topography into the existing terrestrial hydrologic processes in the CoLM to improve runoff predictions in land surface parameterizations. This study has assessed the new CoLM+LF using Earth observations at the 30-km resolution targeted for mesoscale climate applications, especially for surface and subsurface runoff predictions in the Nakdong River Watershed of Korea under study. Both the baseline CoLM and the new CoLM+LF are implemented in a standalone mode using the realistic surface boundary conditions (SBCs) and meteorological forcings constructed from remote sensing products and in situ observations, mainly by geoprocessing tools in a Geographic Information System (GIS) for the study domain. The performance of the CoLM and the CoLM+LF simulations are evaluated by the comparison of daily runoff results from both models with observations during 2009 at the Jindong stream gauge station in the study watershed. The proposed CoLM+LF, which can simulate the effect of runoff travel time over a watershed by an explicit lateral flow scheme, more effectively captures seasonal variations in daily streamflow than the baseline CoLM.
Care With The Potential Organ Donor In The Intensive Care Unit
Authors: Barreto de Carvalho Francisca Patrícia , Morais Oliveira Kênnia Stephanie , Dos Santos Silva Glauber Weder , Nóbrega Melo Geórgia , Enggle de Araújo Alves Thiago , Arrais Ana Cristina , Rocha Leite Ilana Deyse , Lopes Fernandes Amélia Carolina , Clebeson Oliveira Lucidio , Ribeiro Soares Francisco Rafael
Published: February 2017

Introduction: Organ transplants have expanded throughout the country, being extremely significant for the population.

Objective: To know the reality of organ harvesting and describe the care with the potential organ donor in an Intensive Care Unit (ICU) and compare it with the pertinent literature.

Method: It is a research of exploratory and descriptive nature, with a qualitative approach. The data were analyzed through the content analysis idealized by Bardin.

Results: The findings indicate that the resolution of care and procedures with the potential donor is essential to the success of transplants in our country.

Conclusions: Several difficulties have been encountered, as the lack of human and material resources generating impasses in the specific care of the potential organ donor and the lack of provision of continuing education.

Keywords: Organ donation; Intensive Care Unit; Nursing.
Chewing Duration Time Of Various Food Textures In Young Adults
Authors: Coutinho Vitcel Raísa , Fernandes de Oliveira José Fagny , Santos Ciro , Soares Pontes Emerson , De Holanda Ferreira Danilo Augusto , Jordão do Amaral Ana Karênina De Freitas
Published: February 2017

OBJECTIVE: To obtain reference values of chewing time of several food textures in young adults.

METHOD: descriptive study with a quantitative approach that was developed in a clinical reference, being the population composed of 40 young adults between 18-30 years of age, of both genres. As exclusion criteria: those with neuromuscular and / or degenerative diseases or consequences thereof, as well as subjects who were making use of any orthodontic / orthopedic resource. We conducted a dental evaluation, followed by a clinical assessment. One at a time, the following foods were offered: French bread, wafer biscuit, roasted cashews, for voluntary chewing.  To measure the food chewing time, we used a stopwatch, and this collection procedure was filmed. Data analysis was performed by means of the SPSS statistics 20.0 (IBM®) program.

RESULTS: There was a statistically significant difference (p < 0.05) between the medians of chewing time of French bread, wafer biscuit and cashew nuts, which were 33.0s (interquartile amplitude 29.0 - 40.0), 10.0s (interquartile amplitude 8.25-12.0) and 18.5s (interquartile amplitude 15.0-23.75), respectively.

CONCLUSION: The texture of foods influences the length of mastication. The more rigid is the food, the more cycles and mandibular movements, and therefore the longer the duration of chewing.
Planetary Torque in 3D Isentropic Disks
Authors: Fung Jeffrey , Masset Frédéric , Lega Elena , Velasco David
Published: February 2017

Planetary migration is inherently a three-dimensional (3D) problem, because Earth-size planetary cores are deeply embedded in protoplanetary disks. Simulations of these 3D disks remain challenging due to the steep resolution requirements. Using two different hydrodynamics codes, FARGO3D and PEnGUIn, we simulate disk–planet interaction for a one to five Earth-mass planet embedded in an isentropic disk. We measure the torque on the planet and ensure that the measurements are converged both in resolution and between the two codes. We find that the torque is independent of the smoothing length of the planet’s potential (r
s), and that it has a weak dependence on the adiabatic index of the gaseous disk (γ). The torque values correspond to an inward migration rate qualitatively similar to previous linear calculations. We perform additional simulations with explicit radiative transfer using FARGOCA, and again find agreement between 3D simulations and existing torque formulae. We also present the flow pattern around the planets that show active flow is present within the planet’s Hill sphere, and meridional vortices are shed downstream. The vertical flow speed near the planet is faster for a smaller r
s or γ, up to supersonic speeds for the smallest r
s and γ in our study.
Absence of an X-shaped Structure in the Milky Way Bulge Using Mira Variable Stars
Authors: López-Corredoira Martín
Published: February 2017

The stellar density distribution of the bulge is analyzed through one of its tracers. We use oxygen-rich Mira variables from the Catchpole et al. survey and OGLE-III survey as standard candles. The average age of these stars is around 9 Gyr. The population traced by Mira variables matches a boxy bulge prediction, not an X-shaped one, because only one peak is observed in the density along the analyzed lines of sight, whereas the prediction of an X-shape gives two clear peaks.
The Planetary Accretion Shock. I. Framework for Radiation-hydrodynamical Simulations and First Results
Authors: Marleau Gabriel-Dominique , Klahr Hubert , Kuiper Rolf , Mordasini Christoph
Published: February 2017

The key aspect determining the postformation luminosity of gas giants has long been considered to be the energetics of the accretion shock at the surface of the planet. We use one-dimensional radiation-hydrodynamical simulations to study the radiative loss efficiency and to obtain postshock temperatures and pressures and thus entropies. The efficiency is defined as the fraction of the total incoming energy flux that escapes the system (roughly the Hill sphere), taking into account the energy recycling that occurs ahead of the shock in a radiative precursor. We focus in this paper on a constant equation of state (EOS) to isolate the shock physics but use constant and tabulated opacities. While robust quantitative results will have to await a self-consistent treatment including hydrogen dissociation and ionization, the results presented here show the correct qualitative behavior and can be understood from semianalytical calculations. The shock is found to be isothermal and supercritical for a range of conditions relevant to the core accretion formation scenario (CA), with Mach numbers

. Across the shock, the entropy decreases significantly by a few times

. While nearly 100% of the incoming kinetic energy is converted to radiation locally, the efficiencies are found to be as low as roughly 40%, implying that a significant fraction of the total accretion energy is brought into the planet. However, for realistic parameter combinations in the CA scenario, we find that a nonzero fraction of the luminosity always escapes the Hill sphere. This luminosity could explain, at least in part, recent observations in the young LkCa 15 and HD 100546 systems.
The Candidate Progenitor of the Type IIn SN 2010jl Is Not an Optically Luminous Star
Authors: Fox Ori D. , Dyk Schuyler D. Van , Dwek Eli , Smith Nathan , Filippenko Alexei V. , Andrews Jennifer , Arendt Richard G. , Foley Ryan J. , Kelly Patrick L. , Miller Adam A. , Shivvers Isaac
Published: February 2017

A blue source in pre-explosion Hubble Space Telescope (HST)/Wide-Field Planetary Camera 2 (WFPC2) images falls within the 5σ astrometric error circle (∼0.″24) derived from post-explosion ground-based imaging of SN 2010jl. At the time the ground-based astrometry was published, however, the SN had not faded sufficiently for post-explosion HST follow-up observations to determine a more precise astrometric solution and/or confirm if the pre-explosion source had disappeared, both of which are necessary to ultimately disentangle the possible progenitor scenarios. Here we present HST/WFC3 imaging of the SN 2010jl field obtained in 2014, 2015, and 2016 when the SN had faded sufficiently to allow for new constraints on the progenitor. The SN, which is still detected in the new images, is offset by 0.″061 ± 0.″008 (15 ± 2 pc) from the underlying and extended source of emission that contributes at least partially, if not entirely, to the blue source previously suggested as the candidate progenitor in the WFPC2 data. This point alone rules out the possibility that the blue source in the pre-explosion images is the exploding star, but may instead suggest an association with a young (<5–6 Myr) cluster and still argues for a massive (>30 M
⊙) progenitor. We obtain new upper limits on the flux from a single star at the SN position in the pre-explosion WFPC2 and Spitzer/IRAC images that may ultimately be used to constrain the progenitor properties.
Cold CO Gas in the Envelopes of FU Orionis-type Young Eruptive Stars
Authors: Kóspál Á. , Ábrahám P. , Csengeri T. , Henning Th. , Moór A. , Güsten R.
Published: February 2017

FU Orionis-type objects (FUors) are young stellar objects experiencing large optical outbursts due to highly enhanced accretion from the circumstellar disk onto the star. FUors are often surrounded by massive envelopes, which play a significant role in the outburst mechanism. Conversely, the subsequent eruptions might gradually clear up the obscuring envelope material and drive the protostar on its way to become a disk-only T Tauri star. Here we present an APEX 12CO and 13CO survey of eight southern and equatorial FUors. We measure the mass of the gaseous material surrounding our targets, locate the source of the CO emission, and derive physical parameters for the envelopes and outflows, where detected. Our results support the evolutionary scenario where FUors represent a transition phase from envelope-surrounded protostars to classical T Tauri stars.