bluuebunny/crossref_metadata_2025 huggingface dataset:
Community engagement strategies to promote recruitment and participation in clinical research among rural communities: A narrative review
Authors: Brockman Tabetha A. , Shaw Olivia , Wiepert Liana , Nguyen Quang Anh , Kelpin Sydney S. , West Ian , Albertie Monica , Williams Shantel , Abbenyi Adeline , Stephenson Noreen , Almader-Douglas Diana , Patten Christi A.
Published: February 2023

Residents of rural areas are underrepresented in research. The aim of this narrative review was to explore studies describing the effectiveness of community engagement strategies with rural communities to promote participant recruitment and participation in clinical research. Following PRISMA guidelines, this narrative review was conducted in June 2020. Our search strategy was built around keywords that included community-engaged research, rural community, and recruitment strategies into clinical research. Content-related descriptive statistics were summarized. The selected articles were distributed into categories of levels of community engagement: inform, consult, involve, collaborate, or co-lead. The search resulted in 2,473 identified studies of which forty-eight met inclusion criteria. Of these, 47.1% were randomized controlled trials. The most common levels of engagement were consultation (n = 24 studies) and collaboration (n = 15), while very few focused on informing (n = 2) and co-leadership (n = 2). Strategies, limitations, and findings are discussed for each level of community engagement. This narrative addressed a gap in knowledge regarding participant recruitment in rural communities in relation to assistance from community members. Community engagement contributed to the success of the research, especially in recruitment, participation, and building trust and partnership.
Reducing burden and building goodwill for practice-embedded trials: Results of rapid qualitative methods in the preimplementation phase of a community paramedic trial to reduce hospitalizations
Authors: Ridgeway Jennifer L. , Wissler Gerdes Erin O. , Lampman Michelle A. , Smith Olivia A. , McCoy Jessica J. , McCoy Rozalina G.
Published: February 2023

Pragmatic trials aim to generate timely evidence while ensuring feasibility, minimizing practice burden, and maintaining real-world conditions. We conducted rapid-cycle qualitative research in the preimplementation period of a trial evaluating a community paramedic program to shorten and prevent hospitalizations. Between December 2021 and March 2022, interviews (n= 30) and presentations/discussions (n= 17) were conducted with clinical and administrative stakeholders. Two investigators analyzed interview and presentation data to identify potential trial challenges, and team reflections were used to develop responsive strategies. Solutions were implemented prior to the commencement of trial enrollment and were aimed at bolstering feasibility and building ongoing practice feedback loops.
Risk Management in the Artificial Intelligence Act
Authors: Schuett Jonas
Published: February 2023

The proposed Artificial Intelligence Act (AI Act) is the first comprehensive attempt to regulate artificial intelligence (AI) in a major jurisdiction. This article analyses Article 9, the key risk management provision in the AI Act. It gives an overview of the regulatory concept behind the norm, determines its purpose and scope of application, offers a comprehensive interpretation of the specific risk management requirements and outlines ways in which the requirements can be enforced. This article can help providers of high-risk systems to comply with the requirements set out in Article 9. In addition, it can inform revisions of the current draft of the AI Act and efforts to develop harmonised standards on AI risk management.
SHEA/IDSA/APIC Practice Recommendation: Strategies to prevent healthcare-associated infections through hand hygiene: 2022 Update
Authors: Glowicz Janet B. , Landon Emily , Sickbert-Bennett Emily E. , Aiello Allison E. , deKay Karen , Hoffmann Karen K. , Maragakis Lisa , Olmsted Russell N. , Polgreen Philip M. , Trexler Polly A. , VanAmringe Margaret A. , Wood Amber R. , Yokoe Deborah , Ellingson Katherine D.
Published: February 2023

The purpose of this document is to highlight practical recommendations to assist acute-care hospitals in prioritization and implementation of strategies to prevent healthcare-associated infections through hand hygiene. This document updates the Strategies to Prevent Healthcare-Associated Infections in Acute Care Hospitals through Hand Hygiene, published in 2014. This expert guidance document is sponsored by the Society for Healthcare Epidemiology (SHEA). It is the product of a collaborative effort led by SHEA, the Infectious Diseases Society of America, the Association for Professionals in Infection Control and Epidemiology, the American Hospital Association, and The Joint Commission, with major contributions from representatives of a number of organizations and societies with content expertise.
A high-order asymptotic analysis of the Benjamin–Feir instability spectrum in arbitrary depth
Authors: Creedon Ryan P. , Deconinck Bernard
Published: February 2023

We investigate the Benjamin–Feir (or modulational) instability of Stokes waves, i.e. small-amplitude, one-dimensional periodic gravity waves of permanent form and constant velocity, in water of finite and infinite depth. We develop a perturbation method to describe to high-order accuracy the unstable spectral elements associated with this instability, obtained by linearizing Euler's equations about the small-amplitude Stokes waves. These unstable elements form a figure-eight curve centred at the origin of the complex spectral plane, which is parametrized by a Floquet exponent. Our asymptotic expansions of this figure-eight are in excellent agreement with numerical computations as well as recent rigorous results by Bertiet al.(Full description of Benjamin–Feir instability of Stokes waves in deep water, 2021, arXiv:2109.11852) and Bertiet al.(Benjamin–Feir instability of Stokes waves in finite depth, 2022, arXiv:2204.00809). From our expansions, we derive high-order estimates for the growth rates of the Benjamin–Feir instability and for the parametrization of the Benjamin–Feir figure-eight curve with respect to the Floquet exponent. We are also able to compare the Benjamin–Feir and high-frequency instability spectra analytically for the first time, revealing three different regimes of the Stokes waves, depending on the predominant instability.
Aiming at inclusive workplaces: A bibliometric and interpretive review at the crossroads of disability management and human resource management
Authors: Palumbo Rocco , Hinna Alessandro , Fakhar Manesh Mohammad
Published: February 2023

Inclusive workplaces rely on the joint optimization of disability management and human resource management. However, disability management has been predominantly investigated as an independent issue, overlooking its interplay with human resource management. The article delivers a bibliometric and interpretive review of the scholarly debate falling at the crossroad of disability management and human resource management, mapping the state of the art of this study domain. Departing from a knowledge core of 91 papers, 6 research streams were identified through bibliographic coupling. They account for the evolution of disability management from a fix-it initiative aimed at fostering return to work of people with disability towards a holistic management approach targeted at inclusiveness. Aligning the hard and the soft sides of disability management and embedding it in the organizational culture are crucial to enact inclusive workplaces and make organizations able to engage people with disability at work.
What Do “Left Behind Communities” Want? A Qualitative Study in the United Kingdom using Photo Elicitation
Authors: WOOD MATTHEW , ANTOVA IVANKA , FLEAR MARK , HERVEY TAMARA
Published: February 2023

Recent shifts in political support to populist parties worldwide have been linked to the changing preferences of “left behind communities.” Based on apparently growing “left behind” support for populists, some commentators have argued for policy changes including tightened immigration rules coupled with increasing investment in economically deprived areas, particularly in health care. However, left behind communities’ policy preferences are unclear from existing research due to a series of methodological challenges associated with researching polarization and stigmatization. We complement existing research with an innovative photo elicitation methodology covering five field sites in the United Kingdom during 2019, focusing on left behind communities’ policy preferences concerning Brexit. Photo elicitation overcomes methodological challenges associated with emotional attachment and stigmatization. Drawing on 418 interviews with 489 participants, we find that interviewees rejected elite framings suggesting a logical link between Brexit and health care investment, instead articulating policy preferences for health care investment drawing on personal experiences.
Potential overestimation of cognitive impairment because of hearing loss: impact of test modalities on cognitive test scores
Authors: Kim M W , Jin M H , Choi J Y , Kwak M Y
Published: February 2023

Objective

Hearing impairment in older adults may affect cognitive function and increase the risk of dementia. Most cognitive tests are delivered auditorily, and individuals with hearing loss may fail to hear verbal instructions. Greater listening difficulty and fatigue in acoustic conditions may impact test performance. This study aimed to examine the effect of decreased audibility on cognitive screening test performance in older adults.

Method

Older adults (n = 63) with different levels of hearing loss completed a standard auditory Mini-Mental State Examination test and a written version of the test.

Results

Individuals with moderate to moderately severe hearing loss (41–70 dB) performed significantly better on the written (24.34 ± 4.90) than on the standard test (22.55 ± 6.25), whereas scores were not impacted for mild hearing loss (less than 40 dB).

Conclusion

Hearing evaluations should be included in cognitive assessment, and test performance should be carefully interpreted in individuals with hearing loss to avoid overestimating cognitive decline.
Review of management practices of sinogenic intracranial abscesses in children
Authors: Milinis K , Thiagarajan J , Leong S , De S , Sinha A , Sharma R , Sharma S
Published: February 2023

Objective

This study aimed to evaluate the management practices and outcomes in children with sinogenic intracranial suppuration.

Method

This was a retrospective cohort study in a single paediatric tertiary unit that included patients younger than 18 years with radiologically confirmed intracranial abscess, including subdural empyema and epidural or intraparenchymal abscess secondary to sinusitis. Main outcomes studied were rate of return to the operating theatre, length of hospital stay, death in less than 90 days and neurological disability at 6 months.

Results

A cohort of 39 consecutive patients presenting between 2000 and 2020 were eligible for inclusion. Subdural empyema was the most common intracranial complication followed by extradural abscess and intraparenchymal abscess. Mean length of hospital stay was 42 days. Sixteen patients were managed with combined ENT and neurosurgical interventions, 15 patients underwent ENT procedures alone and 4 patients had only neurosurgical drainage. Four patients initially underwent non-operative management. The rates of return to the operating theatre, neurological deficits and 90-day mortality were 19, 9 and 3, respectively, and were comparable across the 4 treatment arms. In the univariate logistic regression, only the size of an intracranial abscess was found be associated with an increased likelihood of return to the operating theatre, whereas combined ENT and neurosurgical intervention did not result in improved outcomes.

Conclusion

Sinogenic intracranial abscesses are associated with significant morbidity and mortality. The size of an intracranial abscess has a strong association with a need for a revision surgery.
Population structure of the fireworm Hermodice carunculata in the wider Caribbean, Atlantic and Mediterranean Sea
Authors: Cruz Ramos Mariel A. , Schizas Nikolaos V.
Published: February 2023

The bearded fireworm Hermodice carunculata is a conspicuous omnivorous benthic invertebrate distributed in the wider Atlantic Ocean, Mediterranean Sea and the Red Sea. Questions were raised in the past regarding the taxonomic status of amphiatlantic fireworm specimens but subsequent analysis of morphological data with new molecular data reconfirmed the presence of only one species, H. carunculata with amphiatlantic distribution. Hermodice carunculata has been the subject of several taxonomic and genetic studies but there are still questions regarding the genetic diversity and population structure of the species throughout its range. We contributed to the genetic studies of H. carunculata with ~200 new samples from over 20 locations in the Caribbean, Eastern Atlantic Ocean and the Mediterranean Sea. We sequenced the mitochondrial markers Cytochrome c oxidase Subunit I (COI) and Cytochrome b (Cytb) from each polychaete to examine the patterns of genetic diversity of H. carunculata. Our data revealed a significant population structure between the Caribbean H. carunculata including those from Brazil compared with those from the eastern Atlantic (Tenerife, Canary Islands) and eastern Mediterranean (Greece and Malta). We inferred rates of gene flow between eastern and western Atlantic populations by estimating the bidirectional effective migration rate (Nem). We corroborate previous studies indicating the existence of one species with genetically distinct populations. However, the biological significance of the observed population divergence should be evaluated with cross-breeding experiments.
A Lyapunov-based robust control for permanent magnet synchronous motor in the modular joint of collaborative robot
Authors: Zhen Shengchao , Li Yangyang , Liu Xiaoli , Wang Jun , Chen Feng , Chen Xiaofei
Published: February 2023

In order to decrease the influence of system parameters and load on the dynamic performance of permanent magnet synchronous motor (PMSM) in cooperative robot joint modules, a practical model-based robust control method was proposed. It inherits the traditional proportional-integral-derivative (PID) control and robust control based on error and model-based control. We first set up the nominal controller using the dynamics model. In order to limit the influence of uncertainty on dynamic performance, a robust controller is established based on Lyapunov method. The control can be regarded as an improved PID control or a redesigned robust control. Compared with the traditional control method, it is simple to implement and has practical effects. It is proved by theoretical analysis that the controller can guarantee the uniform boundedness and uniform final boundedness of the system. In addition, the prototype of fast controller cSPACE is built on the experiment platform, which averts long-time programming and debugging. It offers immense convenience for practical operation. Finally, numerical simulation and real-time experiment results are presented. Based on cSPACE and a PMSM in the joint module of a practical cooperative robot, the availability of the control design and the achievable control performance are verified.
Constitutions and Modernity in Post-Colonial Afghanistan: Ethnolinguistic Nationalism and the Making of an Afghan Nation-State
Authors: Leake Elisabeth
Published: February 2023

In recent decades, the rule of law has not been commonly associated with Afghanistan. Instead, its politics have been more likely to be framed in terms of lawlessness and ungovernability. But this trope does not do justice to Afghanistan's longer history of statehood or experiences of constitution-making. Over the course of the twentieth century, Afghan leaders drafted seven constitutions (in 1923, 1931, 1964, 1976, 1980, 1987, and 1990). These constitutions represented leaders’ attempts to assert their legitimacy and enforce their vision of an Afghan nation-state. This article sheds fresh light on Afghan elites’ top-down framing of Afghan national identity through ethnolinguism, exploring the legalization of Pashto as both an official and national language in Afghanistan's constitutions. Reformers intended Pashto to transgress community, kinship, and regional boundaries and act as a source of unity (though one in which ethnolinguistic minorities had little say). Tracing Afghanistan's constitutional history from 1923–90, this article reveals language as a constitutional arena for debating Afghan modernity and identity. As such, this article integrates Afghanistan into legal histories of South Asia while emphasizing how Afghan constitutionalists engaged in the process of law-making as a means of expressing Afghanistan's own independence and ideas of modernity.
Utilising abattoir sero-surveillance for high-impact and zoonotic pig diseases in Lao PDR
Authors: Matsumoto Nina , Douangngeun Bounlom , Theppangna Watthana , Khounsy Syseng , Phommachanh Phouvong , Toribio Jenny-Ann , Bush Russell D. , Selleck Paul W. , Gleeson Laurence J. , Siengsanan-Lamont Jarunee , Blacksell Stuart D.
Published: February 2023

National disease surveillance systems are essential to a healthy pig industry but can be costly and logistically complex. In 2019, Lao People's Democratic Republic (Lao PDR) piloted an abattoir disease surveillance system to assess for the presence of high impact pig diseases (HIPDs) using serological methods. The Lao Department of Livestock and Fisheries (DLF) identified Classical Swine Fever (CSF), Porcine Respiratory and Reproductive Syndrome (PRRS) and Brucella suis as HIPDs of interest for sero-surveillance purposes. Porcine serum samples (n = 597) were collected from six Lao abattoirs in March to December of 2019. Serological enzyme-linked immunosorbent assay (ELISA) methods were chosen for their high-throughput and relatively low-costs. The true seroprevalence for CSF and PRRS seropositivity were 68.7%, 95% CI (64.8–72.3) and 39.5%, 95% CI (35.7–43.5), respectively. The results demonstrated no evidence of Brucella spp. seroconversion. Lao breed pigs were less likely to be CSF seropositive (P < 0.05), whilst pigs slaughtered at <1 year of age were less likely to be PRRS seropositive (P < 0.01). The testing methods could not differentiate between seropositivity gained from vaccine or natural infection, and investigators were unable to obtain the vaccine status of the slaughtered pigs from the abattoirs. These results demonstrate that adequate sample sizes are possible from abattoir sero-surveillance and lifetime health traceability is necessary to understand HIPDs in Lao PDR.
Exploring readiness for implementing goal-oriented care in primary care using normalization process theory
Authors: Huybrechts Ine , Boeykens Dagje , Grudniewicz Agnes , Steele Gray Carolyn , De Sutter An , Pype Peter , Van de Velde Dominique , Boeckxstaens Pauline , Anthierens Sibyl
Published: February 2023

Aim:

To use normalization process theory (NPT) to build a strategy for the implementation of goal-oriented care (GOC) in primary care in Flanders, Belgium.

Background:

GOC is a possible approach to more coordinated and integrated care and tailors care to patients’ personal life goals. The concept has gained interest among policy makers and researchers, but the main drivers for successful implementation are the primary healthcare professionals (PHCPs) who need to see added value of GOC in order to embed it into their daily practice. NPT, developed to understand the processes of implementing new ways of organizing care, offers a useful lens to understand adoption of GOC in primary care practice.

Method:

PHCPs (n = 131) who participated in a 2-hour community meeting on GOC were asked to complete the Normalization MeAsure Development survey. This 23-item survey is based on NPT and describes participants’ views about how an intervention would impact their work, their expectations about it, and whether it could become a routine part of their work.

Findings:

The NPT constructs coherence (sense-making work) and cognitive participation (relational work) showed positive tendency toward implementation of GOC. The participants had an initial understanding on GOC and there was much interest in supporting and start working with this approach. The other constructs collective action (operational work) and reflexive monitoring (appraisal work) will need further efforts to trigger implementation. A common ground is needed to integrate GOC as a common practice which can be achieved by intensive interprofessional collaboration.
‘Some of my patients only come to renew their prescriptions. They are not interested in any additional advice or support’. Physicians’ perceptions on their roles in cardiovascular diseases risk reduction and management in Fiji
Authors: Kumar Nikansha , Mohammadnezhad Masoud , Narayan Ravneel
Published: February 2023

Background:

Primary health care (PHC) physicians’ perceptions are vital to understand as they are the first-line health care providers in cardiovascular diseases (CVD) risk assessment and management. This study aims to explore PHC physicians’ perceptions on their roles and their perceptions on management and risk reduction approaches on CVD risk reduction and management in Fiji.

Methods:

This is a qualitative study conducted in the Suva Medical area among 7 health centers from 1 August to 31 September, 2021. Purposive sampling was used to recruit physicians who worked in the Suva medical area as PHC physicians with at least 6 months’ experience in the Special Outpatients Department clinics. In-depth interview were conducted using a semi-structured questionnaire over the telephone and recorded on a tablet device application. The interview content was then transcribed, and thematic analysis was done.

Results:

This study included 25 PHC physicians. From the thematic analysis, 2 major themes emerged with 6 subthemes. Theme 1 was CVD management skills with 3 subthemes including education, experience and trainings, beliefs and attitudes of physicians, self-confidence and effectiveness in CVD risk reduction and management. Theme 2 was roles and expectations with 3 subthemes including perceptions of effective treatment, perceptions of physicians’ roles and perceptions of patients’ expectations. Physicians generally see their role as central and imperative. They perceive to be important and leading toward combating CVDs.

Conclusions:

Physicians’ perceptions on their commitment to prevention and management of CVDs through their skills and knowledge, beliefs and motivation should be acknowledged. It is recommended that the physicians are updated on the current evidence-based medicine. Limitations include results that may not be the reflection of the entire physician and multidisciplinary community and the difficulties in face-to-face interviews due to the coronavirus diseases of 2019 pandemic.
Under the Veil of Democracy: What Do People Mean When They Say They Support Democracy?
Authors: Chapman Hannah S. , Hanson Margaret C. , Dzutsati Valery , DeBell Paul
Published: February 2023

Scholars have expressed concern over waning support for democracy worldwide. But what do ordinary citizens mean by the term “democracy," and how do their definitions of democracy influence their support for it? Using global cross-national survey data, this study demonstrates that individual variation in the understanding of democracy is substantively linked to democratic support across countries and regime contexts. Individuals who define democracy in terms of elections and the protection of civil liberties and those with greater conceptual complexity express higher support for democracy. This relationship between democratic conceptualization and support holds across diverse political contexts and alternative explanations. These results suggest that it is essential to consider divergent conceptualizations of democracy—and how they may vary systematically—when analyzing popular opinions of democracy.
The Limits of Lobbying: Null Effects from Four Field Experiments in Two State Legislatures
Authors: Camp Matthew J. , Schwam-Baird Michael , Zelizer Adam
Published: February 2023

It is widely thought that lobbyists exert influence over legislators’ policy positions and, as a result, over policy outcomes. One mechanism of influence is the provision of policy expertise. Yet, there is little credible empirical evidence that lobbyists’ expertise influences legislative outcomes. Across four experiments fielded with three lobbyists in two state legislatures that examine two public measures of legislators’ positions, we find no evidence that lobbyists’ expertise influences legislators’ policy positions. We do find, in contrast, that the same policy expertise treatment is influential when provided by a legislative staffer. We conclude that policy information can influence legislators’ positions, but that legislators are cautious when that information is provided by lobbyists.
Light Under Arctic Sea Ice in Observations and Earth System Models
Authors: Lebrun Marion , Vancoppenolle Martin , Madec Gurvan , Babin Marcel , Becu Guislain , Lourenço Antonio , Nomura Daiki , Vivier Frederic , Delille Bruno
Published: February 2023

The intensity and spectrum of light under Arctic sea ice, key to the energy budget and primary productivity of the Arctic Ocean, are tedious to observe. Earth System Models (ESMs) are instrumental in understanding the large‐scale properties and impacts of under‐ice light. To date, however, ESM parameterizations of radiative transfer have been evaluated with a few observations only. From observational programs conducted over the past decade at four locations in the Northern Hemisphere sea ice zone, 349 observational records of under‐ice light and coincident environmental characteristics were compiled. This data set was used to evaluate seven ESM parameterizations. Snow depth, melt pond presence and, to some extent, ice thickness explain the observed variance in light intensity, in agreement with previous work. The effects of Chlorophyll‐a are also detected, with rather low intensity. The spectral distribution of under‐ice light largely differs from typical open ocean spectra but weakly varies among the 349 records except for a weak effect of snow depth on the blue light contribution. Most parameterizations considered reproduce variations in under‐ice light intensity. Large errors remain for individual records, on average by a factor of ∼3, however. Skill largely improves if more predictors are considered (snow and ponds in particular). Residual errors are attributed to missing physics in the parametrizations, inconsistencies in the model‐observation comparison protocol, and measurement errors. We provide recommendations to improve the representation of light under sea ice in the ice‐ocean model NEMO, which may also apply to other ESMs and help improve next‐generation ESMs.
Impact of Extratropical Northeast Pacific SST on U.S. West Coast Precipitation
Authors: Beaudin É. , Di Lorenzo E. , Miller A. J. , Seo H. , Joh Y.
Published: February 2023

The rainfall over the U.S. West Coast is known to be highly influenced by large‐scale atmospheric circulation and tropical climate teleconnections. However, the role of North Pacific oceanic variability is less understood. Using high‐resolution regional atmospheric model simulations forced by sustained positive and negative phases of the extratropical Pacific Decadal Oscillation sea surface temperature anomalies (SSTa), we diagnose the precipitation changes over the U.S. West Coast during 2010–2020. We find that precipitation anomalies are up to 60% stronger (weaker) for the warm (cold) cases, especially over Northern and Central California during wintertime, and Baja California in the summertime. In both seasons, precipitation is predominantly modulated through changes in the water vapor flux, which are directed toward the coast in wintertime and away from the coast during summertime. These flux anomalies are primarily driven by large‐scale changes in the wind associated with the atmospheric adjustment to the strong ocean SSTa.
A Long‐Term Earthquake Catalog for the Endeavour Segment: Constraints on the Extensional Cycle and Evidence for Hydrothermal Venting Supported by Propagating Rifts
Authors: Krauss Zoe , Wilcock William S. D. , Heesemann Martin , Schlesinger Angela , Kukovica Jacob , Farrugia Joseph J.
Published: February 2023

We use ocean bottom seismometer data from the Endeavour segment of the Juan de Fuca ridge to construct a long‐term earthquake catalog for an intermediate spreading rate mid‐ocean ridge. We present >50,000 new earthquake locations for 2016–2021 from the Ocean Networks Canada NEPTUNE cabled observatory and relocate earthquakes from two autonomous networks in 1995 and 2003–2006. The catalog comprises >85,000 earthquakes located using three‐dimensional segment‐scale P and S wave velocity models from a prior tomography experiment. Despite the small footprints of networks near the segment center, locations show good agreement with geologic features at segment ends. The improved locations show that the northern Endeavour segment ruptured southwards from 48.3°N to 48.05°N during two diking events in early 2005, possibly accompanied by diking on the West Valley (WV) propagator. Persistent off‐axis seismicity near the segment center appears to be related to the WV and Cobb propagating rifts which we infer extend ∼10 km closer to the Endeavour segment center than is apparent in bathymetry. We suggest that the proximity of the propagators to the Endeavour vent fields (VFs) contributes to the localization, vigor, and longevity of the fields by focusing permeability through ongoing fracturing and by limiting extrusive magmatism through degassing of the axial magma lens. Increasing rates of seismicity beneath the VFs beginning in late 2018 and a deepening of earthquakes in 2020 indicate that the central portion of the segment may be entering the later stages of the eruptive cycle.
Microphysical Pathways Active Within Thunderstorms and Their Sensitivity to CCN Concentration and Wind Shear
Authors: Barrett Andrew I. , Hoose Corinna
Published: February 2023

The impact of cloud condensation nuclei (CCN) concentration on microphysical processes within thunderstorms and the resulting surface precipitation is not fully understood yet. In this work, an analysis of the microphysical pathways occurring in these clouds is proposed to systematically investigate and understand these sensitivities. Thunderstorms were simulated using convection‐permitting (1 km horizontal grid spacing) idealized simulations with the ICON model, which included a 2‐moment microphysics parameterization. Cloud condensation nuclei concentrations were increased from 100 to 3,200 CCN/cm3, in five different wind shear environments ranging from 18 to 50 m/s. Large and systematic decreases of surface precipitation (up to 35%) and hail (up to 90%) were found as CCN was increased. Wind shear changes the details, but not the sign, of the sensitivity to CCN. The microphysical process rates were tracked throughout each simulation, closing the mass budget for each hydrometeor class, and collected together into “microphysical pathways,” which quantify the different growth processes leading to surface precipitation. Almost all surface precipitation occurred through the mixed‐phase pathway, where graupel and hail grow by riming and later melt as they fall to the surface. The mixed‐phase pathway is sensitive to CCN concentration changes as a result of changes to the riming rate, which were systematically evaluated. Supercooled water content was almost insensitive to increasing CCN concentration, but decreased cloud drop size led to a large reduction in the riming efficiency (from 0.79 to 0.24) between supercooled cloud drops and graupel or hail, resulting in less surface precipitation.
Negligible role of TRAIL death receptors in cell death upon endoplasmic reticulum stress in B-cell malignancies
Authors: Favaro Francesca , Both Demi , Derks Ingrid A. M. , Spaargaren Marcel , Muñoz-Pinedo Cristina , Eldering Eric
Published: February 2023

Impairments in protein folding in the endoplasmic reticulum (ER) lead to a condition called ER stress, which can trigger apoptosis via the mitochondrial or the death receptor (extrinsic) pathway. There is controversy concerning involvement of the death receptor (DR)4 and DR5-Caspase-8 –Bid pathway in ER stress-mediated cell death, and this axis has not been fully studied in B-cell malignancies. Using three B-cell lines from Mantle Cell Lymphoma, Waldenström’s macroglobulinemia and Multiple Myeloma origins, we engineered a set of CRISPR KOs of key components of these cell death pathways to address this controversy. We demonstrate that DR4 and/or DR5 are essential for killing via TRAIL, however, they were dispensable for ER-stress induced-cell death, by Thapsigargin, Brefeldin A or Bortezomib, as were Caspase-8 and Bid. In contrast, the deficiency of Bax and Bak fully protected from ER stressors. Caspase-8 and Bid were cleaved upon ER-stress stimulation, but this was DR4/5 independent and rather a result of mitochondrial-induced feedback loop subsequent to Bax/Bak activation. Finally, combined activation of the ER-stress and TRAIL cell-death pathways was synergistic with putative clinical relevance for B-cell malignancies.
Opposing effects of clozapine and brexpiprazole on β-aminoisobutyric acid: Pathophysiology of antipsychotics-induced weight gain
Authors: Fukuyama Kouji , Motomura Eishi , Okada Motohiro
Published: February 2023

Clozapine is one of the most effective antipsychotics and has the highest risk of weight gain and metabolic complications; however, the detailed pathophysiology of its clinical action and adverse reactions remains to be clarified. Therefore, the present study determined the chronic effects of clozapine (high risk of weight gain) and brexpiprazole (relatively low risk of weight gain) on intracellular and extracellular levels of β-aminoisobutyric acid (BAIBA) enantiomers, which are endogenous activators of AMP-activated protein kinase (AMPK). L-BAIBA is the dominant BAIBA enantiomer in the rat hypothalamus and cultured astrocytes, whereas L-BAIBA accounts for only approximately 5% of the total plasma BAIBA enantiomers. L-BAIBA displayed GABAB receptor agonistic action in the extracellular space and was released through activated astroglial hemichannels, whereas in the intracellular space, L-BAIBA activated AMPK signalling. Chronic administration of the effective doses of clozapine increased intracellular and extracellular levels of L-BAIBA in the hypothalamus and cultured astrocytes, whereas that of brexpiprazole decreased them. These results suggest that enhancing hypothalamic AMPK signalling by increasing intracellular L-BAIBA levels is, at least partially, involved in the pathophysiology of clozapine-induced weight gain and metabolic complications.
Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis
Authors: Nassour Joe , Aguiar Lucia Gutierrez , Correia Adriana , Schmidt Tobias T. , Mainz Laura , Przetocka Sara , Haggblom Candy , Tadepalle Nimesha , Williams April , Shokhirev Maxim N. , Akincilar Semih C. , Tergaonkar Vinay , Shadel Gerald S. , Karlseder Jan
Published: February 2023

Cancers arise through the accumulation of genetic and epigenetic alterations that enable cells to evade telomere-based proliferative barriers and achieve immortality. One such barrier is replicative crisis—an autophagy-dependent program that eliminates checkpoint-deficient cells with unstable telomeres and other cancer-relevant chromosomal aberrations1,2. However, little is known about the molecular events that regulate the onset of this important tumour-suppressive barrier. Here we identified the innate immune sensor Z-DNA binding protein 1 (ZBP1) as a regulator of the crisis program. A crisis-associated isoform of ZBP1 is induced by the cGAS–STING DNA-sensing pathway, but reaches full activation only when associated with telomeric-repeat-containing RNA (TERRA) transcripts that are synthesized from dysfunctional telomeres. TERRA-bound ZBP1 oligomerizes into filaments on the outer mitochondrial membrane of a subset of mitochondria, where it activates the innate immune adapter protein mitochondrial antiviral-signalling protein (MAVS). We propose that these oligomerization properties of ZBP1 serve as a signal amplification mechanism, where few TERRA–ZBP1 interactions are sufficient to launch a detrimental MAVS-dependent interferon response. Our study reveals a mechanism for telomere-mediated tumour suppression, whereby dysfunctional telomeres activate innate immune responses through mitochondrial TERRA–ZBP1 complexes to eliminate cells destined for neoplastic transformation.
The structural basis for HIV-1 Vif antagonism of human APOBEC3G
Authors: Li Yen-Li , Langley Caroline A. , Azumaya Caleigh M. , Echeverria Ignacia , Chesarino Nicholas M. , Emerman Michael , Cheng Yifan , Gross John D.
Published: February 2023

The APOBEC3 (A3) proteins are host antiviral cellular proteins that hypermutate the viral genome of diverse viral families. In retroviruses, this process requires A3 packaging into viral particles1–4. The lentiviruses encode a protein, Vif, that antagonizes A3 family members by targeting them for degradation. Diversification of A3 allows host escape from Vif whereas adaptations in Vif enable cross-species transmission of primate lentiviruses. How this ‘molecular arms race’ plays out at the structural level is unknown. Here, we report the cryogenic electron microscopy structure of human APOBEC3G (A3G) bound to HIV-1 Vif, and the hijacked cellular proteins that promote ubiquitin-mediated proteolysis. A small surface explains the molecular arms race, including a cross-species transmission event that led to the birth of HIV-1. Unexpectedly, we find that RNA is a molecular glue for the Vif–A3G interaction, enabling Vif to repress A3G by ubiquitin-dependent and -independent mechanisms. Our results suggest a model in which Vif antagonizes A3G by intercepting it in its most dangerous form for the virus—when bound to RNA and on the pathway to packaging—to prevent viral restriction. By engaging essential surfaces required for restriction, Vif exploits a vulnerability in A3G, suggesting a general mechanism by which RNA binding helps to position key residues necessary for viral antagonism of a host antiviral gene.
Respiratory patterns and baroreflex function in heart failure
Authors: Radaelli Alberto , Mancia Giuseppe , Balestri Giulia , Bonfanti Daniela , Castiglioni Paolo
Published: February 2023

Little is known on the effects of respiratory patterns on baroreflex function in heart failure (HF). Patients with HF (n = 30, age 61.6 ± 10 years, mean ± SD) and healthy controls (CNT, n = 10, age 58.9 ± 5.6 years) having their R–R interval (RRI, EKG), systolic arterial blood pressure (SBP, Finapres) and respiratory signal (RSP, Respitrace) monitored, were subjected to three recording sessions: free-breathing, fast- (≥ 12 bpm) and slow- (6 bpm) paced breathing. Baroreflex sensitivity (BRS) and power spectra of RRI, SBP, and RSP signals were calculated. During free-breathing, compared to CNT, HF patients showed a significantly greater modulation of respiratory volumes in the very-low-frequency (< 0.04 Hz) range and their BRS was not significantly different from that of CNT. During fast-paced breathing, when very-low-frequency modulations of respiration were reduced, BRS of HF patients was significantly lower than that of CNT and lower than during free breathing. During slow-paced breathing, BRS became again significantly higher than during fast breathing. In conclusion: (1) in free-breathing HF patients is present a greater modulation of respiratory volumes in the very-low-frequency range; (2) in HF patients modulation of respiration in the very-low and low frequency (around 0.1 Hz) ranges contributes to preserve baroreflex-mediated control of heart rate.
Green synthesis and characterization of silver nanoparticles for reducing the damage to sperm parameters in diabetic compared to metformin
Authors: Ali Iman A. Mohammed , Ahmed Ali Ben , Al-Ahmed Hazim Ismail
Published: February 2023

The present study used physics to synthesize silver nanoparticles using aqueous extract of fresh garlic as reducing and as a stabilizing agent silver nitrate solution. This method has proven to be environmentally friendly and safe for the synthesis of stable silver nanoparticles. The acquisition of silver nanoparticles was confirmed by optical detection, that is, by changing the color of the liquid to transparent orange and then blackish brown. Then, the characterization was confirmed using other assays. In this study, it was found that the absorption peak of silver nanoparticles was at a wavelength of 420 nm and the particle size ranged between [50–350] nm. The surface roughness of silver oxide/silver nanoparticles was 9.32 nm with an average square roughness of 21.19 nm, and the energy dispersive spectra showed that the absorption peak was in the region of 3 keV, indicating that the nanoparticles contained crystalline silver. In this study, the stability of the silver nanoparticles was good, as ZP reached (− 19.5). The results confirm that the conductivity increases with the increase in frequency due to the high energy of the photons, which causes the electrons to vibrate in the energy levels and thus increase the energy in the mitochondria and increase the movement of sperm in the Diabetic mice treated with doses of silver nanoparticles. The toxic effect of silver nanoparticles has been evaluated in other studies, in addition to evaluating antioxidants, antifungals, treating cancer cells, regulating cholesterol levels, the effect of these nanoparticles on sex cells in pregnant female mice, heart tension, and many other tests. In this study, the activities and efficacy of silver nanoparticles on sperms were determined in male mice with diabetes caused by STZ, and the treatment period was long (35 days) so that the evaluation period was a complete life cycle of male sex cells and within a long period of time and at an average nano size. This has not been studied in other previous studies. The results indicate that the biosynthesis of silver nanoparticles using garlic plant led to positive results on sperm treatments by contributing to an increase in the number of sperm with reactivation and a decrease in abnormalities in addition to a decrease in mortality due to diabetes. This is evidence that the synthesis of silver nanoparticles using garlic plant size (50–350 nm) can treat impotence and be used in the future in the treatment of many diseases without side effects.
Larger error negativity peak amplitudes for accuracy versus speed instructions may reflect more neuro-cognitive alignment, not more intense error processing
Authors: Mattes André , Porth Elisa , Niessen Eva , Kummer Kilian , Mück Markus , Stahl Jutta
Published: February 2023

Understanding human error processing is a highly relevant interdisciplinary goal. More than 30 years of research in this field have established the error negativity (Ne) as a fundamental electrophysiological marker of various types of erroneous decisions (e.g. perceptual, economic) and related clinically relevant variations. A common finding is that the Ne is more pronounced when participants are instructed to focus on response accuracy rather than response speed, an observation that has been interpreted as reflecting more thorough error processing. We challenge this wide-spread interpretation by demonstrating that when controlling for the level of non-event-related noise in the participant-average waveform and for single-trial peak latency variability, the significant speed-accuracy difference in the participant-average waveform vanishes. This suggests that the previously reported Ne differences may be mostly attributable to a more precise alignment of neuro-cognitive processes and not (only) to more intense error processing under accuracy instructions, opening up novel perspectives on previous findings.
Spatial helicity response metric to quantify particle size and turbidity of heterogeneous media through circular polarization imaging
Authors: Singh Michael D. , Vitkin I. Alex
Published: February 2023

Backscattered circularly polarized light from turbid media consists of helicity-flipped and helicity-preserved photon sub-populations (i.e., photons of perpendicular and parallel circular handedness). Their intensities and spatial distributions are found to be acutely sensitive to average scatterer size and modestly sensitive to the scattering coefficient (medium turbidity) through an interplay of single and multiple scattering effects. Using a highly sensitive intensified-CCD camera, helicity-based images of backscattered light are captured, which, with the aid of corroborating Monte Carlo simulation images and statistics, enable (1) investigation of subsurface photonic pathways and (2) development of the novel ‘spatial helicity response’ metric to quantify average scatterer size and turbidity of tissue-like samples. An exciting potential application of this work is noninvasive early cancer detection since malignant tissues exhibit alterations in scatterer size (larger nuclei) and turbidity (increased cell density).
Investigation of the acoustic emission and fractal characteristics of coal with varying water contents during uniaxial compression failure
Authors: Ali Muhammad , Wang Enyuan , Li Zhonghui , Khan Naseer Muhammad , Sabri Sabri Mohanad Muayad , Ullah Barkat
Published: February 2023

To investigate the effect of water on the mechanical properties and acoustic emission (AE) characteristics of coal in the failure and deformation processes. Coal samples of different content were subjected to uniaxial compression tests and AE signals were monitored. The characteristics of the AE signals were further analyzed using fractal analysis. The results show that saturated coal samples have substantially reduced mechanical properties such as uniaxial compressive strength (UCS), dissipation energy, peak stress, and elastic modulus. Under loading, stress–strain curves are characterized by five distinct stages: (1) compaction; (2) linear elastic; (3) crack stable propagation; (4) crack accelerating propagation; and (5) post-peak and residual stages. Using phase-space theory, a novel Grassberger Procaccia (GP) algorithm was utilized to find the AE fractal characteristics of coal samples in different stages. It is significant to note that AE energy does not exhibit fractal characteristics in either the first or second stages. Contrary to the first two stages, the third stage showed obvious fractal characteristics. Fractal analysis of AE time sequences indicates that fractal dimension values change as stress increases, indicating the initiation of complex microcracks in coal. In the fourth stage, the fractal dimension rapidly declines as the strength reaches its limit, indicating the occurrence of macrocracks. However, fractal dimensions continued to decrease further or increased slightly in the fifth stage. Consequently, the coal begins to collapse, potentially resulting in a disaster and failure. It is, therefore, possible to accurately predict coal and rock dynamic failures and microcrack mechanisms by observing the subsequent sudden drop in the correlation dimension of the AE signals in response to different stages of loading.