bluuebunny/crossref_metadata_2025 huggingface dataset:
Pendekatan Andragogi dalam Pengajian Sosialita Kota Langsa
Authors: Rais Mukhlis
Published: June 2021

This article discusses the andragogy approach process carried out by the presenter Ustadz when filling out the recitation of socialite mothers who are active in the Taklim Amanah Council and the Taklim Hidayatullah Council, Langsa City, the obstacles when filling out the recitation, and the implications for their social and religious activities in Langsa City. The number of taklim assemblies recorded in the list of recapitulation of the Office of Islamic Shari'a and Dayah Education in Langsa City is 195 taklim assemblies. Officially, these two taklim assemblies have not been included in the list compiled by the Office of the Office of Islamic Shari'a and Dayah Education in Langsa City. However, the recitation activities of the two taklim councils are quite unique. In addition, the congregation is dominated by socialite mothers with a variety of religious materials and full social activities in a week. After carrying out the process of gathering information through in-depth interviews with Ustadz at the Amanah and Hidayatullah Taklim Council and through observation of assistance and participants, this study found that the andragogy approach process in these two taklim assemblies was very special, namely using a dialogical approach, psychological, and religious as contained in the concepts and principles of the andragogy approach. So that the implication in social and religious activities is that the socialites of Langsa City are able to provide positive benefits for people's lives.
An Improvement Of Least Square - Twin Support Vector Machine
Authors: Nguyen Thanh Vi , Nguyễn Thế Cường
Published: June 2021

n binary classification problems, two classes of data seem tobe different from each other. It is expected to be more complicated dueto the number of data points of clusters in each class also be different.Traditional algorithms as Support Vector Machine (SVM), Twin Support Vector Machine (TSVM), or Least Square Twin Support VectorMachine (LSTSVM) cannot sufficiently exploit information about thenumber of data points in each cluster of the data. Which may be effectto the accuracy of classification problems. In this paper, we proposes anew Improved Least Square - Support Vector Machine (called ILS-SVM)for binary classification problems with a class-vs-clusters strategy. Experimental results show that the ILS-SVM training time is faster thanthat of TSVM, and the ILS-SVM accuracy is better than LSTSVM andTSVM in most cases.
Environmental Factors Associated with the Eukaryotic Microbial Community and Microalgal Groups in the Mountain Marshes of South Korea
Authors: KIM YOUNG-SAENG , YUN HYUN-SIK , LEE JEA HACK , KIM HAN-SOON , YOON HO-SUNG
Published: June 2021

The diversity indices of eukaryotic microalgal groups in the Jeonglyeongchi, Waegok, and Wangdeungjae marshes of Mount Jiri, Korea, were measured using Illumina MiSeq and culture-based analyses. Waegok marsh had the highest species richness, with a Chao1 value of 828.00, and the highest levels of species diversity, with Shannon and Simpson index values of 6.36 and 0.94, respectively, while Wangdeungjae marsh had the lowest values at 2.97 and 0.75, respectively. The predominant species in all communities were Phagocata sibirica (Jeonglyeongchi, 68.64%), Aedes albopictus (Waegok, 34.77%), Chaetonotus cf. (Waegok, 24.43%), Eimeria sp. (Wangdeungjae, 26.17%), and Eumonhystera cf. (Wangdeungjae, 22.27%). Relative abundances of the microalgal groups Bacillariophyta (diatoms) and Chlorophyta (green algae) in each marsh were respectively: Jeonglyeongchi 1.38% and 0.49%, Waegok 7.0% and 0.3%, and Wangdeungjae 10.41% and 4.72%. Illumina MiSeq analyses revealed 34 types of diatoms and 13 types of green algae. Only one diatom (Nitzschia dissipata) and five green algae (Neochloris sp., Chlamydomonas sp., Chlorococcum sp., Chlorella vulgaris, Scenedesmus sp.) were identified by a culture-based analysis. Thus, Illumina MiSeq analysis can be considered an efficient tool for analyzing microbial communities. Overall, our results described the environmental factors associated with geographically isolated mountain marshes and their respective microbial and microalgal communities.
Occurrence of Beta-Lactamases in Colistin-Resistant Enterobacterales Strains in Poland – a Pilot Study
Authors: STEFANIUK ELŻBIETA M. , KOZIŃSKA ALEKSANDRA , WAŚKO IZABELA , BARANIAK ANNA , TYSKI STEFAN
Published: June 2021

Sixty-five colistin-resistant Enterobacterales isolates recovered from different clinical specimens were analyzed. The strains were collected in 12 hospitals all over Poland within a period of nine months. Strains were analyzed for eight genes from the mcr family. The presence of mcr-1 gene was detected in three Escherichia coli strains. The 45/65 isolates were identified as ESBL producers. CTX-M-1-like enzymes were the most common ESBLs (n = 40). One E. coli and seven Klebsiella pneumoniae strains produced carbapenemases, with the NDM being produced by five isolates. Among all the strains tested, four and five were resistant to new drugs meropenem/vaborbactam and ceftazidime/avibactam, respectively.
The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing
Authors: TONG TONG , NIU XIAOHUI , LI QIAN , LING YUXI , LI ZUMING , LIU JIA , ZHANG MICHAEL , BAI ZHIHUI , XIA RAN , WU ZHICHAO , LIU XIU
Published: June 2021

Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 108 CFU/ml, a medium-dose group of 109 CFU/ml, and a high-dose group of 1010 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.
Shtisel. Season 3. Directed by Alon Zingman. Written by Ori Elon and Yehonathan Indursky. NMC United Entertainment, [2013-]. Hebrew with English subtitles.
Authors: Weinbaum Batya
Published: June 2021

Review of Shtisel Season 3.
Kraut, Florence Reiss. How to Make a Life: A Novel. She Writes Press. N.P.: 2020
Authors: Mami Fouad
Published: June 2021

Review of Kraut, Florence Reiss. How to Make a Life: A Novel. N.P.: She Writes Press, 2020.
Batalion, Judy. The Light of Days: The Untold Story of Women Resistance Fighters in Hitler’s Ghettos. New York, NY: William Morrow, 2020.
Authors: Weinbaum Batya
Published: June 2021

Review of Batalion, Judy. The Light of Days: The Untold Story of Women Resistance Fighters in Hitler’s Ghettos. New York, NY: William Morrow, 2020.
Optimizing Orthopaedics and Rehabilitation Medicine Course Grading System by Spreadsheet Software
Authors: Aubcherye Pennapa , Aubcherye Narin , Noysang Butsakorn , Woratanarat Patarawan , Laohajaroensombat Sukij
Published: June 2021

Background: Complex processes in grading system occasionally delay time-to-report and affect the accuracy of the report in Orthopaedics and Rehabilitation Medicine Course.

Objective: To assess the effectiveness of spreadsheet software in the grading processes of the Orthopaedics and Rehabilitation Medicine Course.

Methods: A cross-sectional study was conducted at Department of Orthopaedics, Faculty of Medicine Ramathibodi Hospital, Mahidol University. Exam scores of the 5th year medical students between 2014 and 2018 were collected. This study included complete summative data and excluded any data from repeated examinations. The effectiveness of grading system using spreadsheet software was evaluated the primary outcome as time-to-report (days) and accuracy of grade reports.

Results: There were 893 students from 5 academic years, median duration of grade reports was 2.5 days (range, 0 - 30). Grade reports were 100% accurate. The analysis showed the longest duration of time-to-report was in 2014 with median 10.5 days (range, 0 - 30), and the shortest duration was in 2018 with median 0 day (range, 0 - 4). During 5 consecutive years, the spreadsheet software can reduce time-to-report significantly (P = .01).

Conclusions: Spreadsheet software is an effective method to reduce time-to-report and reporting errors in grading system.
Integrated Learning Management Based on Face-to-Face learning and Online Instruction in Primary Medical Care Course for Nursing Students in the New Normal Era
Authors: Palacheewa Natchaya , Tongtham Arbkhanok , Lhimsoonthon Bussarin , Thangkratok Pramote
Published: June 2021

Blended learning refers to the model of integration between face-to-face learning and online instruction. This model used in coronavirus disease 2019 (COVID-19) pandemic, essential for prevention and control the emerging infectious disease transmission, and enhanced achievement of expected learning outcomes among nursing students. This article aimed to present a blended instruction based on asynchronous and synchronous online learning with face-to-face approach in primary medical care course for nursing students in the new normal era. The instructional design based on the 5 components of the ADDIE model including 1) analysis, 2) designs, 3) development, 4) implementation, and 5) evaluation. The conceptual framework for evaluating blended learning outcomes including 1) learners’ satisfaction, 2) students’ engagement, and 3) course outcomes. It was found that the learners’ satisfaction on blended learning were at high level. Blended learning performs a pivotal role in supporting nursing students’ engagement. All nursing students gained higher scores than the pass criteria. This article determined on improving nursing education through blended learning and extended novel issues for further research and development.
Pieces Framework for Analysis of User Saticfaction Internet of Things-Based Devices
Authors: Kakihary Nestiara Lidya
Published: June 2021

Internet of Things is a concept where internet connectivity can exchange information with one another with the existing devices around it. IoT can help humans in their daily activities. In general, Internet of Things-based devices can perform controlling and scheduling. In this research, an analysis of the level of power saving using smart lamp and smart plug devices based on the Internet of Things will be carried out. The analysis carried out in this study used the PIECES method which is a framework that can classify a problem, opportunity, and directives. Research on the efficiency of electricity savings based on IoT devices will be supported using devices and applications from Bardi Smart Home. In addition to the PIECES method, this analysis and research is supported by using the IoT framework: Conceptual Framework, and will be supported by IoT-based devices from Bardi Smart Home.
Sentiment Analisis Terhadap Cryptocurrency Berdasarkan Comment Dan Reply Pada Platform Twitter
Authors: Prasetya Adam , Ferdiansyah Ferdiansyah , Kunang Yesi Novaria , Negara Edi Surya , Chandra Winoto
Published: June 2021

Analisis sentiment saat ini banyak di gunakan masyarat sebagai bahan untuk mengetahui pendapat atau opini masyarakattentang berbagai macam hal. Dengan menggunakan sentiment analisis kita dapat mengklasifikasikan data apakah data tersebuttermasuk opini netral opini positif opini negatif. Penelitian ini membahas tentang analisis sentiment untuk mengukur tingkatakurasi dari pendapat masyarakat pada tiga cryptocurrency yaitu Bitcoin,ethereum,ripple dengan metode Naive Bayes dansupport vector machine yang berguna untuk mengetahui nilai akurasi yang tertinggi dari dua metode yang digunakan dalampenelitian ini. Ada banyak metode yang bisa digunakan untuk mengkasifikasikan opini tersebut, namun penelitian ini dipilihmetode Naive Bayes dan Support vector machine, dengan alasan metede tersebut banyak di gunakan oleh peneliti lain danmenghasilkan nilai akurasi yang tinggi. Hasil dari penelitian ini adalah berupa data perbandingan dari akurasi. hasil akurasidari 3 cryptocurrency SVM lebih besar dari pada nilai akurasi 3 cryptocurrency Naive Bayes.
Rancang Bangun Aplikasi Pemesanan Makanan Berbasis Android
Authors: Setiawan Rudi , Nugroho Wahyu
Published: June 2021

Perkembangan zaman yang semakin modernmenciptakan berbagai macam teknologi, hampir seluruh bidang manusia menggunakan teknologi informasi dan komunikasi yang dapat memberikan transformasi bagi kehidupan manusia akan kebutuhan pertukaran informasi yang cepat dan tepat. Kondisi ini dapat dimanfaatkan oleh pelaku Usaha Mikro Kecil dan Menengah untuk memasarkan produk – produknya, khususnya dalam bidang kuliner atau makanan. Oleh karena itu diperlukan sebuah teknologi sistem informasi khususnya berbasis Androidsebagai upaya dalam peningkatan pelayanan dalam Usaha Mikro Kecil dan Menengah sehingga transaksi yang dilakukan lebih cepat dan tepat tanpa perlu pelanggan untuk datang ke merchantatau outlet. Pengembangan sistem ini dilakukan dengan metode Waterfall, pemodelan yang digunakan yaitu Unified Modeling Language. Bahasa pemrograman yang digunakan yaitu Dartdengan framework fluttersebagai front-end androiddan PHPsebagai koneksi database(Back-end) serta databaseyang digunakan adalah MySQL. Hasil pengujian aplikasi didapatkan nilai interpretasi sebesar 90.95% yang menandakan bahwa aplikasi yang dibangun sangat layak untuk diterapkan.
Perencanaan Strategi Sistem Informasi Dengan Metode Ward And Peppard di Perusahaan Toko Surabaya cabang Surakarta
Authors: Cahyo Adiatama , Manuputty Augie David
Published: June 2021

The role of information technology is very important for the progress of a company, by using information technology, strategic planning for the company's development will be better, some companies will definitely take advantage of information technology for the progress of their companies, this is also used for Toko baju Surabaya cabang Surakarta. This shop is a company engaged in the sale of clothing, this company uses the SmartConsole application to support sales. However, there are still sectors in this company that have not implemented an information system, one of which is in the section on stock data collection and payroll. Therefore, an appropriate IS / IT strategic planning is needed. This research was conducted using the Ward and Peppard method with SWOT analysis, Five Forces Porter, and McFarlan Strategic Grid. The output produced from this study is in the form of strategic recommendations from the results of the internal and external environmental analysis of the company's information system, as well as a proposal for an Information System to be mapped using the McFarlan Strategic Grid to be applied to companies in the next two years.
Perencanaan Arsitektur Enterprise Menggunakan TOGAF ADM (Studi Kasus: Kantor Desa Lembang)
Authors: Angeline Desy , Fibriani Charitas
Published: June 2021

The Lembang Village Office is a government institution that operates at the Village level in the Bengkayang Regency area. The Lembang Village Government has not implemented an Information System in carrying out its duties, this can be seen from the obstacles faced by the Village government. The obstacles faced are the frequent accumulation of old data, so it is difficult when it will be recorded again, even usually there is data lost so it requires a system that can help data storage. The purpose of this research is to provide directions or directions to be able to implement an information system for data storage. performance in the future, the method used this time is TOGAF (The Open Group Architecture Framework) ADM (Architecture Development Method).This research will produce a blueprint and provide a roadmap using the elements that exist in the togaf at the Lembang Village Office in order to implement an information system on future performance.
Fenntartható élelmiszer-fogyasztás a Debreceni Egyetem hallgatóinak körében
Authors: Bauerné Gáthy Andrea , Szűcs István
Published: June 2021

Mind a hazai mind a nemzetközi szakirodalomban egyetértés van abban, hogy az egészségtudatosság és a környezettudatosság együttesen jellemzi a fenntartható élelmiszer-fogyasztást. A vizsgálat jelen esetben a felnövő generáció élelmiszer-fogyasztási szokásainak felmérésére irányult, a mostani 18-25 éves korosztály tekinthető a „közeljövő fogyasztójának”, a vizsgált korcsoportban a felsőoktatási hallgatók esetében elmondható, hogy a fogyasztásaik során, különösen az élelmiszer-fogyasztás területén önálló döntéshozónak tekinthetőek. Ezen életszakaszban kialakuló fogyasztási minták – melyet az oktatásból szerzett információk is befolyásolnak – meghatározóak lehetnek a későbbiekre is. A felmérésünk alapján megállapítható, hogy a Debreceni Egyetem hallgatói kevésbé ismerik a környezettudatosság kritériumait, mint az egészségtudatosság jellemzőit, inkább tekintik magukat egészségtudatos élelmiszer-fogyasztónak, mint környezettudatos élelmiszer-fogyasztónak, s nagyobb arányban utasítják el az élelmiszer-fogyasztás kapcsán a környezeti szempontokat, inkább az önérdek dominál a mások érdekeivel szemben a döntéseiknél. Kiemelendő, hogy a fentiek minden esetben a vélt, saját magukról véleményezett egészségtudatosságra és környezettudatosságra tett megállapítások. A továbbiakban azt szándékozzuk vizsgálni, hogy a vélt és a valós tudatosság között milyen összefüggés fedezhető fel az élelmiszer-fogyasztás tekintetében.
Effectiveness of biologic treatment of psoriasis vulgaris (certolizumab pegol in practice of dermatologist)
Authors: Rudnevа N. S. , Sadunashvili T. G. , Chumakova Ya. Yu. , Natarova E. V.
Published: June 2021

The work represents the prospective cohort analysis of patients with psoriasis treated with certolizumab pegol (CZP) at the Tula Regional Clinical Dermatovenerologic Dispensary in years 2017–2019, who achieved remission and discontinued CZP and real-time observation of the patients. The patients remained in sustained remission after discontinuation of certolizumab pegol (mean drug-free remission was 42 weeks). Patients who had not responded to systemic therapy prior to CZP treatment demonstrated good response to methotrexate and cyclosporin A, which suggests the modulation of immune response by certolizumab pegol. The obtained results demonstrate that intermittent treatment with certolizumab pegol effiiently controls psoriasis, reduces drug burden.
Stimuli-Responsive Polymeric Nanoplatforms for Cancer Therapy
Authors: Chang Di , Ma Yuanyuan , Xu Xiaoxuan , Xie Jinbing , Ju Shenghong
Published: June 2021

Polymeric nanoparticles have been widely used as carriers of drugs and bioimaging agents due to their excellent biocompatibility, biodegradability, and structural versatility. The principal application of polymeric nanoparticles in medicine is for cancer therapy, with increased tumor accumulation, precision delivery of anticancer drugs to target sites, higher solubility of pharmaceutical properties and lower systemic toxicity. Recently, the stimuli-responsive polymeric nanoplatforms attracted more and more attention because they can change their physicochemical properties responding to the stimuli conditions, such as low pH, enzyme, redox agents, hypoxia, light, temperature, magnetic field, ultrasound, and so on. Moreover, the unique properties of stimuli-responsive polymeric nanocarriers in target tissues may significantly improve the bioactivity of delivered agents for cancer treatment. This review introduces stimuli-responsive polymeric nanoparticles and their applications in tumor theranostics with the loading of chemical drugs, nucleic drugs and imaging molecules. In addition, we discuss the strategy for designing multifunctional polymeric nanocarriers and provide the perspective for the clinical applications of these stimuli-responsive polymeric nanoplatforms.
A New Electrochemical Detection Technique for Organic Matter Content in Ecological Soils
Authors: Liu Jinping , Yang Tao , Xu Jiaqi , Sun Yankun
Published: June 2021

The rapid detection of organic matter in soil is of great interest in agriculture, but the commonly used techniques require laboratory operation. Therefore, the development of a technique that allows rapid detection of soil organic matter in the field is of great interest. In this work, we propose an electrochemical-based approach for the detection of organic matter in soil particles. Since soil particles immobilized directly on the electrode surface can fall off during testing, we introduced graphene to coat the soil particles. The encapsulated soil particles can be stably immobilized on the electrode surface. We have investigated the electrochemical behavior of soil particles. The results show a correspondence between the electrochemical oxidation and reduction of soil particles and the organic matter content in them. We collected soil samples from three sites and constructed an electrochemical modeling, testing framework with stability based on multiple calibrations and random division of the prediction set. We used the equal interval partial least squares (EC-PLS) method for potential optimization to establish the equivalent model set. A joint model for the electrochemical analysis of organic matter in three locations of soil samples was developed for the commonality study.
Modification of the SnO2 Electron Transporting Layer by Using Perylene Diimide Derivative for Efficient Organic Solar Cells
Authors: Kong Tianyu , Wang Rui , Zheng Ding , Yu Junsheng
Published: June 2021

Recently, tin oxide (SnO2) nanoparticles (NPs) have attracted considerable attention as the electron transporting layer (ETL) for organic solar cells (OSCs) due to their superior electrical properties, excellent chemical stability, and compatibility with low-temperature solution fabrication. However, the rough surface of SnO2 NPs may generate numerous defects, which limits the performance of the OSCs. In this study, we introduce a perylene diimide derivative (PDINO) that could passivate the defects between SnO2 NP ETL and the active layer. Compared with the power conversion efficiency (PCE) of the pristine SnO2 ETL–based OSCs (12.7%), the PDINO-modified device delivers a significantly increased PCE of 14.9%. Overall, this novel composite ETL exhibits lowered work function, improved electron mobility, and reduced surface defects, thus increasing charge collection efficiency and restraining defect-caused molecular recombination in the OSC. Overall, this work demonstrates a strategy of utilizing the organic–inorganic hybrid ETL that has the potential to overcome the drawbacks of SnO2 NPs, thereby developing efficient and stable OSCs.
A Robust Recovery of Ni From Laterite Ore Promoted by Sodium Thiosulfate Through Hydrogen-Thermal Reduction
Authors: Liu Shoujun , Yang Chao , Yang Song , Yu Zhongliang , Wang Zhao , Yan Kang , Li Jin , Liu Xingyang
Published: June 2021

Laterite ore is one of the important sources of nickel (Ni). However, it is difficult to liberate Ni from ore structure during reduction roasting. This paper provided an effective way for a robust recovery of Ni from laterite ore by H2 reduction using sodium thiosulfate (Na2S2O3) as a promoter. . It was found that a Ni content of 9.97% and a Ni recovery of 99.24% were achieved with 20 wt% Na2S2O3 at 1,100°C. The promoting mechanism of Na2S2O3 in laterite ore reduction by H2 was also investigated. The thermogravimetric results suggested the formation of Na2Mg2SiO7, Na2SO3, Na2SO4, and S during the pyrolysis of laterite with Na2S2O3, among which the alkali metal salts could destroy the structures of nickel-bearing silicate minerals and hence release Ni, while S could participate in the formation of the low-melting-point eutectic phase of FeS-Fe. The formation of low-melting-point phases were further verified by the morphology analysis, which could improve the aggregation of Ni-Fe particles due to the capillary forces of FeS-Fe as well as the enhanced element migration by the liquid phase of sodium silicates during reduction.
Eruption Style, Emplacement Dynamics and Geometry of Peralkaline Ignimbrites: Insights From the Lajes-Angra Ignimbrite Formation, Terceira Island, Azores
Authors: Pimentel Adriano , Self Stephen , Pacheco José M. , Jeffery Adam J. , Gertisser Ralf
Published: June 2021

Ignimbrites are relatively uncommon on ocean island volcanoes and yet they constitute a significant portion of the stratigraphy of Terceira Island (Azores). The Lajes-Angra Ignimbrite Formation (ca. 25 cal ka BP) contains the youngest ignimbrites on Terceira and records two ignimbrite-forming eruptions of Pico Alto volcano that occurred closely spaced in time. Here, we present the first detailed lithofacies analysis and architecture of the Angra and Lajes ignimbrites, complemented by petrographic, mineral chemical, whole rock and groundmass glass geochemical data. The two ignimbrites have the same comenditic trachyte composition, but show considerable variability in trace element and groundmass glass compositions, revealing complex petrogenetic processes in the Pico Alto magma reservoir prior to eruption. The Angra Ignimbrite has a high-aspect ratio and is massive throughout its thickness. It was formed by a small-volume but sustained pyroclastic density current (PDC) fed by a short-lived, low pyroclastic fountain. Overall, the PDC had high particle concentration, granular fluid-based flow conditions and was mostly channelled into a valley on the south part of Terceira. By contrast, the Lajes Ignimbrite has a low-aspect ratio and shows vertical and lateral lithofacies variations. It was formed by a sustained quasi-steady PDC generated from vigorous and prolonged pyroclastic fountaining. The ignimbrite architecture reveals that depositional conditions of the parent PDC evolved as the eruption waxed. The dilute front of the current rapidly changed to a high particle concentration, granular fluid-based PDC that extended to the north and south coasts, with limited capacity to surmount topographic highs. Contrary to what is commonly assumed, the low-aspect ratio of the Lajes Ignimbrite is interpreted to result from deposition of a relatively low velocity PDC over a generally flat topography. This work highlights that the geometry (aspect ratio) of ignimbrites does not necessarily reflect the kinetic energy of PDCs and thus should not be used as a proxy for PDC emplacement dynamics. Although the probability of an ignimbrite-forming eruption on Terceira is relatively low, such a scenario should not be underestimated, as a future event would have devastating consequences for the island’s 55,000 inhabitants.
Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Sepsis-Induced Acute Kidney Injury by Promoting Mitophagy of Renal Tubular Epithelial Cells via the SIRT1/Parkin Axis
Authors: Guo Jun , Wang Rong , Liu Donghai
Published: June 2021

Sepsis is a common risk factor for acute kidney injury (AKI). Bone marrow-derived mesenchymal stem cells (BMSCs) bear multi-directional differentiation potential. This study explored the role of BMSCs in sepsis-induced AKI (SI-AKI). A rat model of SI-AKI was established through cecal ligation and perforation. The SI-AKI rats were injected with CM-DiL-labeled BMSCs, followed by evaluation of pathological injury of kidney tissues and kidney injury-related indicators and inflammatory factors. HK-2 cells were treated with lipopolysaccharide (LPS) to establish SI-SKI model in vitro. Levels of mitochondrial proteins, autophagy-related proteins, NLRP3 inflammasome-related protein, and expressions of Parkin and SIRT1 in renal tubular epithelial cells (RTECs) of kidney tissues and HK-2 cells were detected. The results showed that BMSCs could reach rat kidney tissues and alleviate pathological injury of SI-SKI rats. BMSCs inhibited inflammation and promoted mitophagy of RTECs and HK-2 cells in rats with SI-AKI. BMSCs upregulated expressions of Parkin and SIRT1 in HK-2 cells. Parkin silencing or SIRT1 inhibitor reversed the promoting effect of BMSCs on mitophagy. BMSCs inhibited apoptosis and pyroptosis of RTECs in kidney tissues by upregulating SIRT1/Parkin. In conclusion, BMSCs promoted mitophagy and inhibited apoptosis and pyroptosis of RTECs in kidney tissues by upregulating SIRT1/Parkin, thereby ameliorating SI-AKI.
Case Report: Malignant Pheochromocytoma Without Hypertension Accompanied by Increment of Serum VEGF Level and Catecholamine Cardiomyopathy
Authors: Kaneto Hideaki , Kamei Shinji , Tatsumi Fuminori , Shimoda Masashi , Kimura Tomohiko , Nakanishi Shuhei , Miyaji Yoshiyuki , Nagai Atsushi , Kaku Kohei , Mune Tomoatsu
Published: June 2021

Introduction

Pheochromocytoma is a catecholamine-producing tumor in the adrenal medulla and is often accompanied by hypertension, hyperglycemia, hypermetabolism, headache, and hyperhidrosis, and it is classified as benign and malignant pheochromocytoma. In addition, persistent hypertension is often observed in subjects with malignant pheochromocytoma.

Case Presentation

A 52-year-old Japanese male was referred and hospitalized in our institution. He had a health check every year and no abnormalities had been pointed out. In addition, he had no past history of hypertension. In endocrinology markers, noradrenaline level was as high as 7,693 pg/ml, whereas adrenaline level was within normal range. Abdominal contrast-enhanced computed tomography revealed a 50-mm hyper-vascularized tumor with calcification in the right adrenal gland and multiple hyper-vascularized tumors in the liver. In 131I MIBG scintigraphy, there was high accumulation in the right adrenal gland and multiple accumulation in the liver and bone. In echocardiography, left ventricular ejection fraction was as low as 14.3%. In coronary angiography, however, there was no significant stenosis in the coronary arteries. Based on these findings, we finally diagnosed him as malignant pheochromocytoma accompanied by multiple liver and bone metastases and catecholamine cardiomyopathy. However, blood pressure was continuously within normal range without any anti-hypertensive drugs. Right adrenal tumor resection was performed together with left hepatic lobectomy and cholecystectomy. Furthermore, serum levels of vascular endothelial growth factor (VEGF) and parathyroid (PTH)-related protein were very high before the operation but they were markedly reduced after the operation.

Conclusions

This is the first report showing the time course of serum VEGF level in a subject with malignant pheochromocytoma, clearly showing that malignant pheochromocytoma actually secreted VEGF. In addition, this case report clearly shows that we should bear in mind once again that malignant pheochromocytoma is not necessarily accompanied by hypertension.
An Integrated Approach for the Assessment of Environmental Sustainability in Agro-Industrial Waste Management Practices: The Case of the Tequila Industry
Authors: Díaz-Vázquez Diego , Carrillo-Nieves Danay , Orozco-Nunnelly Danielle A. , Senés-Guerrero Carolina , Gradilla-Hernández Misael Sebastián
Published: June 2021

Sustainable waste management is a key component needed to achieve multiple of the Sustainable Development Goals established by the United Nations for the year 2030. Such waste management can reduce the degradation of superficial water sources and can contribute to the sustainable and efficient use of resources. Due to their usually high nutrient and organic loads, agro-industrial wastes pose a threat to soil and water resources and are a major contributor to greenhouse gas generation. In this study, a novel approach was proposed to assess the environmental sustainability of vinasse management practices, integrating an extensive physicochemical characterization of tequila vinasses, a GIS-based weighted overlay analysis and a scenario analysis. Mathematical models were applied for the determination of discharged pollutants (nitrogen, phosphorus, BOD5), as well as greenhouse gas emissions. This methodology was applied to an environmental sustainability assessment of the management practices of tequila vinasses, which are one of the main waste streams generated by the agro-industry in the Mexican state of Jalisco and are one of the main contributors to organic and nutrient loads affecting the water quality of Jalisco’s superficial water sources. Through this integrated approach, critical regions for the management of tequila vinasses were determined and an extensive physicochemical characterization of tequila vinasses was performed and applied to assess the environmental sustainability of four management scenarios for tequila vinasses. These results can be used by decision-makers for the implementation of public policy and infrastructure for the improvement of local and regional waste management systems. Additionally, these data may be used to increase the environmental sustainability of the tequila industry. The proposed methodology has the potential to be applied to different waste-intensive regions as well as different organic waste streams for the assessment of the environmental sustainability of specific management practices considering the local and regional context.
Pharmacogenomics of Hypersensitivity to Non-steroidal Anti-inflammatory Drugs
Authors: Trinh Hoang Kim Tu , Pham Le Duy , Le Kieu Minh , Park Hae-Sim
Published: June 2021

Non-steroidal anti-inflammatory drugs (NSAIDs) are extensively prescribed in daily clinical practice. NSAIDs are the main cause of drug hypersensitivity reactions all over the world. The inhibition of cyclooxygenase enzymes by NSAIDs can perpetuate arachidonic acid metabolism, shunting to the 5-lipoxygenase pathway and its downstream inflammatory process. Clinical phenotypes of NSAID hypersensitivity are diverse and can be classified into cross-reactive or selective responses. Efforts have been made to understand pathogenic mechanisms, in which, genetic and epigenetic backgrounds are implicated in various processes of NSAID-induced hypersensitivity reactions. Although there were some similarities among patients, several genetic polymorphisms are distinct in those exhibiting respiratory or cutaneous symptoms. Moreover, the expression levels, as well as the methylation status of genes related to immune responses were demonstrated to be involved in NSAID-induced hypersensitivity reactions. There is still a lack of data on delayed type reactions. Further studies with a larger sample size, which integrate different genetic pathways, can help overcome current limitations of gen etic/epigenetic studies, and provide valuable information on NSAID hypersensitivity reactions.
Association of Extracellular Signal-Regulated Kinase Genes With Myopia: A Longitudinal Study of Chinese Children
Authors: Xiao Haishao , Lin Shudan , Jiang Dandan , Lin Yaoyao , Liu Linjie , Zhang Qiqi , He Juan , Chen Yanyan
Published: June 2021

Graphical Abstract

The genes in the miRNA-target gene network represent the intersection of the target genes and the genes from String that had direct or indirect interaction relationships with significant genes.
Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4
Authors: Tan Juan , Pan Weinan , Chen Huilin , Du Yafang , Jiang Peiyong , Zeng Dianmei , Wu Jie , Peng Kuang
Published: June 2021

Circular RNA (circRNA) is an important factor for regulating the progression of many cardiovascular diseases, including acute myocardial infarction (AMI). However, the role of circ_0124644 in AMI progression remains unclear. Hypoxia was used to induce cardiomyocytes injury. The expression of circ_0124644, microRNA (miR)-590-3p, and SRY-box transcription factor 4 (SOX4) mRNA was measured by qRT-PCR. Cell counting kit 8 (CCK8) assay and flow cytometry were utilized to detect cell viability, cell cycle progression, and apoptosis. The protein levels of apoptosis markers and SOX4 were determined by western blot (WB) analysis, and the levels of oxidative stress markers were assessed using commercial Assay Kits. Dual-luciferase reporter assay, RIP assay, and RNA pull-down assay were employed to confirm the interaction between miR-590-3p and circ_0124644 or SOX4. Circ_0124644 was upregulated in AMI patients and hypoxia-induced cardiomyocytes. Hypoxia could inhibit cardiomyocytes viability, cell cycle process, and promote apoptosis and oxidative stress, while silencing circ_0124644 could alleviate hypoxia-induced cardiomyocytes injury. In terms of mechanism, circ_0124644 could target miR-590-3p. MiR-590-3p overexpression could relieve hypoxia-induced cardiomyocytes injury. Also, the suppressive effect of circ_0124644 knockdown on hypoxia-induced cardiomyocytes injury could be reversed by miR-590-3p inhibitor. Moreover, SOX4 was found to be a target of miR-590-3p, and its overexpression also could reverse the regulation of miR-590-3p on hypoxia-induced cardiomyocytes injury. Circ_0124644 silencing could alleviate hypoxia-induced cardiomyocytes injury by regulating the miR-590-3p/SOX4 axis, suggesting that it might be a target for alleviating AMI.
Circulating and Synovial Pentraxin-3 (PTX3) Expression Levels Correlate With Rheumatoid Arthritis Severity and Tissue Infiltration Independently of Conventional Treatments Response
Authors: Boutet Marie-Astrid , Nerviani Alessandra , Lliso-Ribera Gloria , Leone Roberto , Sironi Marina , Hands Rebecca , Rivellese Felice , Del Prete Annalisa , Goldmann Katriona , Lewis Myles J. , Mantovani Alberto , Bottazzi Barbara , Pitzalis Costantino
Published: June 2021

Aims

To determine the relationship between PTX3 systemic and synovial levels and the clinical features of rheumatoid arthritis (RA) in a cohort of early, treatment naïve patients and to explore the relevance of PTX3 expression in predicting response to conventional-synthetic (cs) Disease-Modifying-Anti-Rheumatic-Drugs (DMARDs) treatment.

Methods

PTX3 expression was analyzed in 119 baseline serum samples from early naïve RA patients, 95 paired samples obtained 6-months following the initiation of cs-DMARDs treatment and 43 healthy donors. RNA-sequencing analysis and immunohistochemistry for PTX3 were performed on a subpopulation of 79 and 58 synovial samples, respectively, to assess PTX3 gene and protein expression. Immunofluorescence staining was performed to characterize PTX3 expressing cells within the synovium.

Results

Circulating levels of PTX3 were significantly higher in early RA compared to healthy donors and correlated with disease activity at baseline and with the degree of structural damages at 12-months. Six-months after commencing cs-DMARDs, a high level of PTX3, proportional to the baseline value, was still detectable in the serum of patients, regardless of their response status. RNA-seq analysis confirmed that synovial transcript levels of PTX3 correlated with disease activity and the presence of mediators of inflammation, tissue remodeling and bone destruction at baseline. PTX3 expression in the synovium was strongly linked to the degree of immune cell infiltration, the presence of ectopic lymphoid structures and seropositivity for autoantibodies. Accordingly, PTX3 was found to be expressed by numerous synovial cell types such as plasma cells, fibroblasts, vascular and lymphatic endothelial cells, macrophages, and neutrophils. The percentage of PTX3-positive synovial cells, although significantly reduced at 6-months post-treatment as a result of global decreased cellularity, was similar in cs-DMARDs responders and non-responders.

Conclusion

This study demonstrates that, early in the disease and prior to treatment modification, the level of circulating PTX3 is a reliable marker of RA activity and predicts a high degree of structural damages at 12-months. In the joint, PTX3 associates with immune cell infiltration and the presence of ectopic lymphoid structures. High synovial and peripheral blood levels of PTX3 are associated with chronic inflammation characteristic of RA. Additional studies to determine the mechanistic link are required.
Live Virus Neutralisation of the 501Y.V1 and 501Y.V2 SARS-CoV-2 Variants following INO-4800 Vaccination of Ferrets
Authors: Riddell Shane , Goldie Sarah , McAuley Alexander J. , Kuiper Michael J. , Durr Peter A. , Blasdell Kim R. , Tachedjian Mary , Druce Julian D. , Smith Trevor R. F. , Broderick Kate E. , Vasan Seshadri S.
Published: June 2021

The ongoing COVID-19 pandemic has resulted in significant global morbidity and mortality on a scale similar to the influenza pandemic of 1918. Over the course of the last few months, a number of SARS-CoV-2 variants have been identified against which vaccine-induced immune responses may be less effective. These “variants-of-concern” have garnered significant attention in the media, with discussion around their impact on the future of the pandemic and the ability of leading COVID-19 vaccines to protect against them effectively. To address concerns about emerging SARS-CoV-2 variants affecting vaccine-induced immunity, we investigated the neutralisation of representative ‘G614’, ‘501Y.V1’ and ‘501Y.V2’ virus isolates using sera from ferrets that had received prime-boost doses of the DNA vaccine, INO-4800. Neutralisation titres against G614 and 501Y.V1 were comparable, but titres against the 501Y.V2 variant were approximately 4-fold lower, similar to results reported with other nucleic acid vaccines and supported by in silico biomolecular modelling. The results confirm that the vaccine-induced neutralising antibodies generated by INO-4800 remain effective against current variants-of-concern, albeit with lower neutralisation titres against 501Y.V2 similar to other leading nucleic acid-based vaccines.
RIG-I-Like Receptor-Mediated Recognition of Viral Genomic RNA of Severe Acute Respiratory Syndrome Coronavirus-2 and Viral Escape From the Host Innate Immune Responses
Authors: Kouwaki Takahisa , Nishimura Tasuku , Wang Guanming , Oshiumi Hiroyuki
Published: June 2021

RIG-I-like receptors (RLR), RIG-I and MDA5, are cytoplasmic viral RNA sensors that recognize viral double-stranded RNAs and trigger signals to induce antiviral responses, including type I interferon production. Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) caused the coronavirus disease 2019 pandemic. However, the RLR role in innate immune response to SARS-CoV-2 has not been fully elucidated. Here, we studied the roles of RLR in cytokine expression responding to SARS-CoV-2 and found that not only MDA5 but also RIG-I are involved in innate immune responses in some types of human cells. Transfection of total RNAs extracted from SARS-CoV-2-infected cells into epithelial cells induced IFN-β, IP-10, and Ccl5 mRNA expression. The cytokine expression was reduced by knockout of either RIG-I or MDA5, suggesting that both proteins are required for appropriate innate immune response to SARS-CoV-2. Two viral genomic RNA regions strongly induced type I IFN expression, and a 200-base fragment of viral RNA preferentially induced type I IFN in a RIG-I-dependent manner. In contrast, SARS-CoV-2 infectious particles hardly induced cytokine expression, suggesting viral escape from the host response. Viral 9b protein inhibited RIG-I and MAVS interaction, and viral 7a protein destabilized the TBK1 protein, leading to attenuated IRF-3 phosphorylation required for type I IFN expression. Our data elucidated the mechanism underlying RLR-mediated response to SARS-CoV-2 infection and viral escape from the host innate immune response.
Complement Plays a Critical Role in Inflammation-Induced Immunoprophylaxis Failure in Mice
Authors: Escamilla-Rivera Vicente , Santhanakrishnan Manjula , Liu Jingchun , Gibb David R. , Forsmo James E. , Foxman Ellen F. , Eisenbarth Stephanie C. , Luckey C. John , Zimring James C. , Hudson Krystalyn E. , Stowell Sean R. , Hendrickson Jeanne E.
Published: June 2021

Complement impacts innate and adaptive immunity. Using a model in which the human KEL glycoprotein is expressed on murine red blood cells (RBCs), we have shown that polyclonal immunoprophylaxis (KELIg) prevents alloimmunization to transfused RBCs when a recipient is in their baseline state of heath but with immunoprophylaxis failure occurring in the presence of a viral-like stimulus. As complement can be detected on antibody coated KEL RBCs following transfusion, we hypothesized that recipient complement synergizes with viral-like inflammation to reduce immunoprophylaxis efficacy. Indeed, we found recipient C3 and C1q were critical to immunoprophylaxis failure in the setting of a viral-like stimulus, with no anti-KEL IgG alloantibodies generated in C3-/-or C1q-/-mice following KELIg treatment and KEL RBC transfusion. Differences in RBC uptake were noted in mice lacking C3, with lower consumption by splenic and peripheral blood inflammatory monocytes. Finally, no alloantibodies were detected in the setting of a viral-like stimulus following KELIg treatment and KEL RBC transfusion in mice lacking complement receptors (CR1/2-/-), narrowing key cells for immunoprophylaxis failure to those expressing these complement receptors.In-vitrostudies showed complement fixed opsonized RBCs were significantly less likely to bind to B-cells from CR1/2-/-than wild type mice, potentially implicating lowered B-cell activation threshold in the presence of complement as being responsible for these findings. We thus propose a two-hit model for inflammation-induced immunoprophylaxis failure, where the first “hit” is recipient inflammation and the second “hit” is complement production/sensing. These results may have translational relevance to antigen-antibody interactions in humans.
New Therapeutic Tools to Shape Monocyte Functional Phenotypes in Leishmaniasis
Authors: Vellozo Natália S. , Rigoni Thaís S. , Lopes Marcela F.
Published: June 2021

In the innate immunity toLeishmaniainfection tissue-resident macrophages and inflammatory monocytes accumulate host-cell, effector, and efferocytosis functions. In addition, neutrophils, as host, effector, and apoptotic cells, as well as tissue-resident and monocyte-derived dendritic cells (DCs) imprint innate and adaptive immunity toLeishmaniaparasites. Macrophages develop phenotypes ranging from antimicrobial M1 to parasite-permissive M2, depending on mouse strain,Leishmaniaspecies, and T-cell cytokines. The Th1 (IFN-γ) and Th2 (IL-4) cytokines, which induce classically-activated (M1) or alternatively-activated (M2) macrophages, underlie resistance versus susceptibility to leishmaniasis. While macrophage phenotypes have been well discussed, new developments addressed the monocyte functional phenotypes inLeishmaniainfection. Here, we will emphasize the role of inflammatory monocytes to access how potential host-directed therapies for leishmaniasis, such as all-trans-retinoic acid (ATRA) and the ligand of Receptor Activator of Nuclear Factor-Kappa B (RANKL) might modulate immunity toLeishmaniainfection, by directly targeting monocytes to develop M1 or M2 phenotypes.