bluuebunny/crossref_metadata_2025 huggingface dataset:
A framework of computer vision-enhanced microfluidic approach for automated assessment of the transient sickling kinetics in sickle red blood cells
Authors: Qiang Yuhao , Xu Mengjia , Pochron Mira Patel , Jupelli Madhulika , Dao Ming
Published: March 2024

The occurrence of vaso-occlusive crisis greatly depends on the competition between the sickling delay time and the transit time of individual sickle cells, i.e., red blood cells from sickle cell disease (SCD) patients, while they are traversing the circulatory system. Many drugs for treating SCD work by inhibiting the polymerization of sickle hemoglobin (HbS), effectively delaying the sickling process in sickle cells (SS RBCs). Most previous studies on screening anti-sickling drugs, such as voxelotor, rely on in vitro testing of sickling characteristics, often conducted under prolonged deoxygenation for up to 1 hour. However, since the microcirculation of RBCs typically takes less than 1 minute, the results of these studies may be less accurate and less relevant for in vitro-in vivo correlation. In our current study, we introduce a computer vision-enhanced microfluidic framework designed to automatically capture the transient sickling kinetics of SS RBCs within a 1-min timeframe. Our study has successfully detected differences in the transient sickling kinetics between vehicle control and voxelotor-treated SS RBCs. This approach has the potential for broader applications in screening anti-sickling therapies.
Crop detection technologies, mechanical weeding executive parts and working performance of intelligent mechanical weeding: a review
Authors: Xiang Meiqi , Qu Minghao , Wang Gang , Ma Zhongyang , Chen Xuegeng , Zhou Zihao , Qi Jiangtao , Gao Xiaomei , Li Hailan , Jia Honglei
Published: March 2024

Weeding is a key link in agricultural production. Intelligent mechanical weeding is recognized as environmentally friendly, and it profoundly alleviates labor intensity compared with manual hand weeding. While intelligent mechanical weeding can be implemented only when a large number of disciplines are intersected and integrated. This article reviewed two important aspects of intelligent mechanical weeding. The first one was detection technology for crops and weeds. The contact sensors, non-contact sensors and machine vision play pivotal roles in supporting crop detection, which are used for guiding the movements of mechanical weeding executive parts. The second one was mechanical weeding executive part, which include hoes, spring teeth, fingers, brushes, swing and rotational executive parts, these parts were created to adapt to different soil conditions and crop agronomy. It is a fact that intelligent mechanical weeding is not widely applied yet, this review also analyzed the related reasons. We found that compared with the biochemical sprayer, intelligent mechanical weeding has two inevitable limitations: The higher technology cost and lower working efficiency. And some conclusions were commented objectively in the end.
Representations of the Quebec nation through reactions to Bills 21 and 96: an analysis of editorials and Op-eds in English-language media
Authors: Mathieu Félix , Hart Alina
Published: March 2024

This article examines the representations of the Quebec nation in English-language media, specifically focusing on editorials and Op-eds which discuss Bill 21 An Act Respecting the Laicity of the State and Bill 96 An Act Respecting French, the Official and Common Language of Quebec. The study explores the perspectives expressed by members of the dominant English-speaking group in response to these nation-building policies introduced by Quebec public officials between 2019 and 2023. Specifically, it asks: How do editorials and Op-eds in established English-language newspapers represent the Quebec nation in response to Bill 21 and 96? In doing so, the research uncovers how the Quebec nation and its nation-building enterprises are portrayed in the media by examining four established newspapers: the Globe and Mail, the Winnipeg Free Press, the Montreal Gazette, and the Calgary Herald. Mobilizing a qualitative and inductive critical content analysis approach, it highlights core common themes and key normative and regional variations in the perspective advanced by the media outlets.
Psychometric properties of the modified Suicide Stroop Task (M-SST) in patients with suicide risk and healthy controls
Authors: Gold Helena , Stein Maria , Glaesmer Heide , Spangenberg Lena , Strauss Maria , Schomerus Georg , Stengler Katarina , Brüdern Juliane
Published: March 2024

The Cognitive Model of Suicide proposes a suicide attentional bias in individuals with suicidal thoughts and behavior (STBs). The Suicide Stroop Task (SST) was developed as a behavioral measure to assess this attentional bias. However, prior studies demonstrated poor psychometric properties of the SST.

Methods

We developed a modified Suicide Stroop Task (M-SST) and tested its psychometric properties in a sample of healthy controls (n = 30) and inpatients with STBs (n = 24). Participants (50% female, aged 18 to 61 years) completed the M-SST with neutral, positive, negative, suicide-related positive and suicide-related negative words. Interference scores were calculated by subtracting the mean reaction time (mean RT) of the neutral words from the mean RT of the suicide-related positive words (mean RTSuicide-Positive–mean RTNeutral) and suicide-related negative words (mean RTSuicide-Negative–mean RTNeutral), resulting in two suicide-specific interference scores. Similarly, interference scores were calculated for the positive and negative words by subtracting the mean RT of neutral words from the mean RT of positive and negative words.

Results

When analyzed separately, patients with STBs showed greater interferences for suicide-related positive words (p = 0.039), and for suicide-related negative words (p = 0.016), however, we found no group differences in interference scores for positive and negative words, suggesting a suicide attentional bias in patients with STBs. Controlling for the repeated measure design, a repeated measure ANOVA failed to detect a significant group × interference interaction effect (p = 0.176), which limits the generalizability of the findings. However, the interference score of suicide-related negative words showed an adequate classification accuracy (AUC = 0.72, 95% CI [0.58–0.86], p = 0.006) for differentiating between healthy controls and patients with STBs. Moreover, the interference scores showed acceptable internal reliability for the total sample and only suicide-related interference scores were correlated with clinical characteristics, thus demonstrating convergent validity.

Conclusion

The results provide preliminary evidence for a suicide attentional bias in individuals with STBs compared to healthy controls. The M-SST represents a promising tool for assessing a suicide attentional bias by revealing adequate psychometric properties. Future studies with larger samples are needed to confirm these preliminary findings.
Quantifying empirical support for theories of consciousness: a tentative methodological framework
Authors: Kirkeby-Hinrup Asger
Published: March 2024

Understanding consciousness is central to understanding human nature. We have competing theories of consciousness. In interdisciplinary consciousness studies most believe that consciousness can be naturalized (i.e., consciousness depends in some substantial way on processes in — or states of — the brain). For roughly two decades, proponents of almost every theory have focused on collecting empirical support for their preferred theory, on the tacit assumption that empirical evidence will resolve the debates. Yet, it remains unclear how empirical evidence can do this in practice. Here I address this issue by offering (a sketch of) a methodology to quantify the divergent sets of empirical support proposed in favor of extant theories of consciousness. This in turn forms the foundation for a process of inference to the best explanation inspired by Bayesian confirmation theory. In interdisciplinary consciousness studies we are blessed with an abundance of theories, but we have reached a point where, going forward, it would be beneficial to focus on the most promising ones. Methods for assessment and comparison are necessary to identify which those are. While future refinement is likely, the methodology for assessment and comparison proposed here is a first step toward a novel way of approaching this through a quantification of empirical support for theories of consciousness.
Psychological capital, mindfulness, and teacher burnout: insights from Chinese EFL educators through structural equation modeling
Authors: Liu Dongxia , Du Ruikang
Published: March 2024

Objective

This cross-sectional study employs Structural Equation Modeling (SEM) to examine the relationships among psychological capital, mindfulness, and teacher burnout in a sample of 387 Chinese English as a Foreign Language (EFL) educators.

Methods

Self-reported data were analyzed to investigate the direct and indirect effects of psychological capital on teacher burnout, with mindfulness serving as a potential mediator.

Results

Our SEM analysis reveals a significant direct negative association between psychological capital and teacher burnout. Moreover, mindfulness significantly mediates the relationship between psychological capital and burnout, indicating that higher psychological capital leads to increased mindfulness, which subsequently reduces burnout.

Conclusion

This study underscores the importance of psychological capital and mindfulness in mitigating teacher burnout among Chinese EFL educators. The findings suggest that interventions targeting both psychological capital and mindfulness practices could bolster teacher well-being and foster a more positive educational environment.
Gamification in the classroom: Kahoot! As a tool for university teaching innovation
Authors: Aibar-Almazán Agustín , Castellote-Caballero Yolanda , Carcelén-Fraile María del Carmen , Rivas-Campo Yulieth , González-Martín Ana María
Published: March 2024

Objectives

The purpose of this study has been to evaluate the use of gamification in the classroom, in terms of its effects on attention, concentration, creativity, and generic capabilities, for university students enrolled in a Bachelor’s degree program in Physiotherapy.

Methods

An experimental design was implemented, using three groups differentiated by their time of exposure to the game (0 min, 30 min, or 60 min per week). The sample consisted of 73 s-year students from a Bachelor’s degree program in Physiotherapy. The theoretical content for each class was taught during a period of 4 months, reinforced by use of the Kahoot! Online platform. Selective attention and concentration were evaluated using the d2 Test of Attention; creative intelligence using the Creative Intelligence Test (CREA); and generic capabilities using the capabilities subscale of the Student Engagement Questionnaire (SEQ).

Results

The study’s participants had a mean age of 19.51 ± 0.9 years, and it has demonstrated that use of Kahoot! For longer periods of time, i.e., more than 60 min per day, can improve essential skills in university students, such as attention, creativity, critical thinking, self-managed learning, adaptability, problem solving, and computer literacy. This study’s results show that integrating Kahoot! Into the educational environment, especially with longer sessions that allow for deeper immersion in the game, produces benefits by stimulating various cognitive aspects and enhancing complex skills.

Conclusion

This study has demonstrated that use of Kahoot! Improves key skills such as attention, creativity, and critical thinking, especially when longer sessions are used. It is also suggested that its use should be balanced with other educational activities, in order to achieve comprehensive development for the students.
Differences in attentional function between experienced mindfulness meditators and non-meditators
Authors: Luo Xiaohui , Zhao Jia , Zhao Dongfang , Wang Li , Hou Yi , Liu Yong , Zeng Jing , Yuan Hong , Lei Xu
Published: March 2024

Introduction

Attentional enhancement has often been identified as the central cognitive mechanism underlying the benefits of mindfulness meditation. However, the extent to which this enhancement is observable in the neural processes underlying long-term meditation is unclear. This current study aimed to examine differences in attentional performance between meditators and controls (non-meditators) using a visual oddball task with concurrent electroencephalography (EEG) recordings.

Methods

Thirty-four participants were recruited, including 16 meditators and 18 healthy controls, who were non-meditators. The participants completed a visual oddball task, using visual stimuli, and EEG recording.

Results

Self-reports revealed that meditators had higher mindful attention scores than did the control group. The behavioral results showed that the meditators demonstrated faster reaction times than the non-meditators did. Neural findings indicated a higher P2 amplitude in the meditators than in the controls. The meditators demonstrated a significantly higher P3 in the target trials than in the distractor trials, which was not observed in the controls. Additionally, the time-frequency analysis demonstrated that the delta and theta powers in the meditators were significantly higher than those in the controls.

Conclusions

The study suggests the meditators exhibited greater attentional performance than the controls did, as revealed by EEG and behavioral measures. This study extends previous research on the effects of mindfulness meditation on attention and adds to our understanding of the effects of long-term mindfulness meditation.
Evaluating the rate of reversal of fentanyl-induced respiratory depression using a novel long-acting naloxone nanoparticle, cNLX-NP
Authors: Averick Saadyah E. , Kassick Andrew J. , Song Daihyun , Zhang Borui , Vigliaturo Jennifer , Luengas Diego , Silva-Ortiz Pedro , Pravetoni Marco , Raleigh Michael D.
Published: March 2024

Introduction

Fentanyl and fentanyl analogs (F/FA) have become increasingly common adulterants in counterfeit prescription pills and illicit street drug mixtures due to their ease of synthesis and exceedingly high potency. The ongoing epidemic of fatal overdoses fueled by F/FA continues to highlight the need for longer-acting therapies than naloxone (NLX), the current gold-standard for reversing opioid overdoses, which shows limited efficacy to prevent renarcotization associated with F/FA toxicity. A novel opioid reversal agent based on covalent naloxone nanoparticles (cNLX-NP) has been shown to blunt fentanyl-induced respiratory depression out to 48 hr, demonstrating its potential therapeutic utility. The purpose of this study was to characterize how rapidly cNLX-NP reverses fentanyl-induced respiratory effects as well as the duration of its protective effects.

Methods

Sprague Dawley male rats (n=6/group) were tested on an oximeter for baseline percent arterial oxygen saturation (%SaO2) challenged with 0.1 mg/kg SC fentanyl and 15 min later given 10 mg/kg IM doses of NLX, nalmefene (NLMF), or cNLX-NP and continuously monitored via oximetry for 10 minutes. One week later the experiment was repeated using a 1:1 mixture of NLX:cNLX-NP as the reversal agent in the rats that previously received NLX alone.

Results

While both NLX and NLMF rapidly reversed %SaO2 to baseline within 1 min, rats that received cNLX-NP did not return to >90% SaO2 levels until 9 min after administration. Similarly, heart and breath rates returned to baseline within 1 min of treatment with NLX and NLMF but did not return to baseline until 10 minutes after cNLX-NP administration. In contrast, NLX:cNLX-NP reversed all fentanyl-induced respiratory depressive effects within one minute.

Discussion

While cNLX-NP alone may not sufficiently reverse F/FA overdose in a timely manner, mixing free NLX with cNLX-NP can provide a mechanism to both rapidly reverse fentanyl-related effects and maintain extended protection against synthetic opioid toxicity. These data support further development of cNLX-NP as a fast-acting and long-lasting antidote to treat F/FA-induced respiratory depression and overdose, and potentially prevent renarcotization in humans.
Socioeconomic inequality in timing of ANC visit among pregnant women in Ethiopia, 2019
Authors: Kidie Atitegeb Abera , Asmamaw Desale Bihonegn , Belachew Tadele Biresaw , Fetene Samrawit Mihret , Baykeda Tsegaw Amare , Endawkie Abel , Zegeye Alebachew Ferede , Tamir Tadesse Tarik , Wubante Sisay Maru , Fentie Elsa Awoke , Negash Wubshet Debebe , Addis Banchilay
Published: March 2024

Background

Antenatal care (ANC) remains an invaluable approach to preventive care for ensuring maternal and infant health outcomes. Women in sub-Saharan Africa tend to delay their first antenatal care visits. In Ethiopia, only 20% of women received their first antenatal care during the first trimester of pregnancy. Timely and appropriate antenatal care practices can potentially save the lives of both mothers and children. Understanding socioeconomic inequality in the timing of antenatal care visits and its determinants may contribute to tackling disparities and achieving the sustainable development goals for maternal health.

Objective

This study aimed to assess the socioeconomic inequality in the timing of antenatal care visit.

Method

Secondary data sourced from the Mini Ethiopian Demographic Health Survey 2019 were used for this study. A total of 2,906 pregnant women were included in the study, and concentration curves were used to show inequality among sociodemographic and economic variables. Decomposition analysis was performed to estimate the contribution of each independent variable to the inequality in the timing of antenatal care visits.

Result

The estimate of early initiation of antenatal care was 63%. The concentration index was 0.18 (P < 0.001). The inequality in the timing of antenatal care visit was more concentrated among the wealthiest pregnant women with a concentration index value of 0.18 (P < 0.001). Based on decomposition analysis results, the wealth index (81.9%.), education status (22.29%), and region (0.0642%) were identified as contributing factors to the inequality in the timing of antenatal care visits among women.

Conclusion

The wealth index, educational status, and region were significant contributors to inequality in the early initiation of antenatal care visit. Improving women's wealth and education and narrowing the inequality gap are crucial for improving the health status of women and their children. We should focus on interventions targeted at early antenatal care visit to address the determinants of socioeconomic inequities.
Longitudinal associations between the frequency of playing Mahjong and cognitive functioning among older people in China: evidence from CLHLS, 2008–2018
Authors: Zhu Lan , Wang Yixi , Wu Yuju , Wilson Amanda , Zhou Huan , Li Ningxiu , Wang Yuanyuan
Published: March 2024

Background

Cognitive decline is prevalent among older adults, often resulting in decreased capabilities for self-care and a diminished quality of life. Mahjong, a culturally cherished and extensively played intellectual game in China, demands considerable cognitive function. While the cognitive benefits of playing Mahjong have been widely accepted, this study investigates an under explored aspect and aimed to ascertain the game’s potential contributions toward bolstering self-care abilities, enhancing overall quality of life, and mitigating against rising societal healthcare costs.

Methods

The data analyzed in the study is collected from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) with cognitive functioning being assessed through the Mini-Mental State Examination (MMSE). The frequency of playing Mahjong was measured through a self-reported questionnaire. Multiple linear regression models, latent variable growth models, and cross-lagged models were used to investigate the longitudinal relationship between game frequency and cognitive function in older people.

Results

Of the 7,535 participants, the mean (SD) age was 81.96 (10.53) years. There were 7,308 (97%), 4,453 (59%), and 1,974 (26%) participants in 2011, 2014, and 2018, respectively. The results showed that Mahjong players had significantly higher MMSE scores compared to non-players from 2008 to 2018 (β = 0.893; p < 0.001), and non-players had significantly lower scores in 2011, 2014, and 2018 than in 2008 (β = −1.326, −0.912, −0.833; Ps > 0.05). Moreover, the frequency of playing Mahjong was associated with improved various cognitive domains. The declining frequency of playing Mahjong was substantially associated with the declining rate of MMSE scores (r = 0.336; p < 0.001). Mahjong frequency showed positive effects on MMSE scores, while the influence of Mahjong on MMSE scores were not significant.

Conclusion

Playing Mahjong has a positive influence on the cognitive functioning among older people. It can help buffer against the decline in cognitive function and maintain cognitive function levels. The higher frequency of playing Mahjong is associated with improved reaction, attention and calculation, and self-coordination. A decline in the frequency of playing Mahjong was associated with a declining rate of cognitive function. The higher frequency of playing Mahjong among older people unilaterally influenced the improvement of cognitive function levels in older people in China.
Health literacy mediates the relationships of cognitive and physical functions with health-related quality of life in older adults
Authors: Chow Bik C. , Jiao Jiao , Duong Tuyen V. , Hassel Holger , Kwok Timothy C. Y. , Nguyen Minh H. , Liu Huaxuan
Published: March 2024

Background

Declining cognitive function (CF) and physical function (PF) relate to poorer health-related quality of life (HRQoL) in older adults. As health literacy (HL) facilitates health information utilization, it may mediate links between functionality and HRQoL appraisals. This study examined HL as an intermediary between joint CF and PF contributions and HRQoL in Hong Kong older adults.

Methods

490 older adults aged 50–80 years completed assessments from March to July 2021. Health Literacy Survey Questionnaire Short Form 12 questions (HLS-SF12), Montreal Cognitive Assessment (MoCA), Senior Fitness Test (SFT) and 12-Item Short-Form Health Survey version 2 (SF-12v2) were used to assess HL, CF, PF and HRQoL, respectively. Path analysis tested a model with HL mediating CF/PF predictors and HRQoL outcome.

Results

Results for direct effects indicated that CF significantly associated with PF (β = 0.115, SE = 0.012, p < 0.001), PF significantly connected to HL (β = 0.101, SE = 0.022, p < 0.001), and HL significantly related to HRQoL (β = 0.457, SE = 0.049, p < 0.001). Meanwhile, PF significantly linked to HRQoL directly (β = 0.156, SE = 0.025, p < 0.001) as well as indirectly (β = 0.046, 95% CI [0.028, 0.067]). Significant mediating effect of HL was found on the relationship of PF and HRQoL.

Conclusion

Findings confirm CF and PF joint impacts on HL and HRQoL in older adults, elucidating HL’s mediating role in translating functionality levels into HRQoL. Fostering enduring health knowledge access may thus buffer effects of age-related declines on well-being. Results can inform interventions leveraging this pathway to promote resilient trajectories.
Forging the path to precision medicine in Qatar: a public health perspective on pharmacogenomics initiatives
Authors: Bastaki Kholoud , Velayutham Dinesh , Irfan Areeba , Adnan Mohd , Mohammed Sawsan , Mbarek Hamdi , Qoronfleh M. Waild , Jithesh Puthen Veettil
Published: March 2024

Pharmacogenomics (PGx) is an important component of precision medicine that promises tailored treatment approaches based on an individual’s genetic information. Exploring the initiatives in research that help to integrate PGx test into clinical setting, identifying the potential barriers and challenges as well as planning the future directions, are all important for fruitful PGx implementation in any population. Qatar serves as an exemplar case study for the Middle East, having a small native population compared to a diverse immigrant population, advanced healthcare system, national genome program, and several educational initiatives on PGx and precision medicine. This paper attempts to outline the current state of PGx research and implementation in Qatar within the global context, emphasizing ongoing initiatives and educational efforts. The inclusion of PGx in university curricula and healthcare provider training, alongside precision medicine conferences, showcase Qatar’s commitment to advancing this field. However, challenges persist, including the requirement for population specific implementation strategies, complex genetic data interpretation, lack of standardization, and limited awareness. The review suggests policy development for future directions in continued research investment, conducting clinical trials for the feasibility of PGx implementation, ethical considerations, technological advancements, and global collaborations to overcome these barriers.
Determination of in vitro immunotoxic potencies of a series of perfluoralkylsubstances (PFASs) in human Namalwa B lymphocyte and human Jurkat T lymphocyte cells
Authors: Janssen Aafke W. F. , Jansen Holleboom Wendy , Rijkers Deborah , Louisse Jochem , Hoekstra Sjoerdtje A. , Schild Sanne , Vrolijk Misha F. , Hoogenboom Ron L. A. P. , Beekmann Karsten
Published: March 2024

Exposure to PFASs is associated to several adverse health effects, such as immunotoxicity. Immunotoxic effects of PFOA and PFOS, including a reduced antibody response in both experimental animals and humans, have been reported. However, there is limited understanding of the underlying mechanisms involved. Moreover, there is only a restricted amount of immunotoxicity data available for a limited number of PFASs. In the current study the effects of 15 PFASs, including short- and long-chain perfluorinated carboxylic and sulfonic acids, fluorotelomer alcohols, and perfluoralkyl ether carboxylic acids were studied on the expression of recombinant activating gene 1 (RAG1) and RAG2 in the Namalwa human B lymphoma cell line, and on the human IL-2 promotor activity in Jurkat T-cells. Concentration-response data were subsequently used to derive in vitro relative potencies through benchmark dose analysis. In vitro relative potency factors (RPFs) were obtained for 6 and 9 PFASs based on their effect on RAG1 and RAG2 gene expression in Namalwa B-cells, respectively, and for 10 PFASs based on their inhibitory effect on IL-2 promotor activity in Jurkat T-cells. The most potent substances were HFPO-TA for the reduction of RAG1 and RAG2 gene expression in Namalwa cells (RPFs of 2.1 and 2.3 respectively), and PFDA on IL-2 promoter activity (RPF of 9.1). RAG1 and RAG2 play a crucial role in V (D)J gene recombination, a process for acquiring a varied array of antibodies crucial for antigen recognition. Hence, the effects observed in Namalwa cells might indicate a PFAS-induced impairment of generating a diverse range of B-cells essential for antigen recognition. The observed outcomes in the Jurkat T-cells suggest a possible PFAS-induced reduction of T-cell activation, which may contribute to a decline in the T-cell dependent antibody response. Altogether, the present study provides potential mechanistic insights into the reported PFAS-induced decreased antibody response. Additionally, the presented in vitro models may represent useful tools for assessing the immunotoxic potential of PFASs and prioritization for further risk assessment.
Knowledge, awareness and perception about equine glanders among veterinarians and medical professionals in India
Authors: Raj Ana , Pathak Anubha , Karuppusamy Shanmugasundaram , Tripathi Bhupendra Nath , Tripathi Hema , Singha Harisankar
Published: March 2024

Glanders is a highly infectious and notifiable disease of equines that occurs due to Burkholderia mallei. In India, glanders re-emerged in 2006 and thereafter regular outbreaks have been reported in various states (n = 14). Frequent and prolonged contact with equids with glanders may transmit B. mallei infection to humans. This study was designed to learn more about the Knowledge, Awareness and Perception (KAP) of veterinarians, para veterinarians, and physicians about equine glanders, which will help in enhancing the nation-wide glanders eradication programme. A total of 165 respondent’s from 11 Indian states and one union territory were surveyed. Most of the respondents (n = 160) were from equine glanders affected or endemic states. Knowledge gap analysis revealed that 40.3 and 22% of the participants were not aware of government regulations and the transmission of glanders, respectively. These are major concerns given the wide spread occurrence of disease in the country. Awareness test on glanders revealed that 65(39.4%) participants would collect biological samples for laboratory confirmation, 67(40.6%) would inform the concerned authorities and 106 (64.2%) replied that they would eliminate the glanders infected equines. Analysis of perception towards equine glanders showed that majority of the participants (n = 113, 68.4%) observed that equine keepers were reluctant to disclose the clinical symptoms of B. mallei infection. Furthermore, non-co-operation and unwillingness by superiors (33.9%), financial (31%), administrative (28.4%), and technical limitations (27.8%) were major constraints under the perception analysis. This study reveals that veterinarians need to be educated on governmental policies and guidelines on equine glanders with regular training and awareness programs. Intersectoral co-ordination to investigate human glanders is also needed.
Review state-of-the-art of output-based methodological approaches for substantiating freedom from infection
Authors: Meletis Eleftherios , Conrady Beate , Hopp Petter , Lurier Thibaut , Frössling Jenny , Rosendal Thomas , Faverjon Céline , Carmo Luís Pedro , Hodnik Jaka Jakob , Ózsvári László , Kostoulas Polychronis , van Schaik Gerdien , Comin Arianna , Nielen Mirjam , Knific Tanja , Schulz Jana , Šerić-Haračić Sabina , Fourichon Christine , Santman-Berends Inge , Madouasse Aurélien
Published: March 2024

A wide variety of control and surveillance programmes that are designed and implemented based on country-specific conditions exists for infectious cattle diseases that are not regulated. This heterogeneity renders difficult the comparison of probabilities of freedom from infection estimated from collected surveillance data. The objectives of this review were to outline the methodological and epidemiological considerations for the estimation of probabilities of freedom from infection from surveillance information and review state-of-the-art methods estimating the probabilities of freedom from infection from heterogeneous surveillance data. Substantiating freedom from infection consists in quantifying the evidence of absence from the absence of evidence. The quantification usually consists in estimating the probability of observing no positive test result, in a given sample, assuming that the infection is present at a chosen (low) prevalence, called the design prevalence. The usual surveillance outputs are the sensitivity of surveillance and the probability of freedom from infection. A variety of factors influencing the choice of a method are presented; disease prevalence context, performance of the tests used, risk factors of infection, structure of the surveillance programme and frequency of testing. The existing methods for estimating the probability of freedom from infection are scenario trees, Bayesian belief networks, simulation methods, Bayesian prevalence estimation methods and the STOC free model. Scenario trees analysis is the current reference method for proving freedom from infection and is widely used in countries that claim freedom. Bayesian belief networks and simulation methods are considered extensions of scenario trees. They can be applied to more complex surveillance schemes and represent complex infection dynamics. Bayesian prevalence estimation methods and the STOC free model allow freedom from infection estimation at the herd-level from longitudinal surveillance data, considering risk factor information and the structure of the population. Comparison of surveillance outputs from heterogeneous surveillance programmes for estimating the probability of freedom from infection is a difficult task. This paper is a ‘guide towards substantiating freedom from infection’ that describes both all assumptions-limitations and available methods that can be applied in different settings.
Genetic diversity of vector-borne zoonotic pathogens in companion dogs and cats, Tianjin, China
Authors: Jian Rui , Xue Jing , Xu Ze-Yun , Chen Si-Si , Wang Fang-Ni , Du Luanying , Xie Guang-Cheng , Guo Wen-Ping
Published: March 2024

Background

Dogs and cats are the hosts of many vector-borne human pathogens that can be transmitted to humans. Given their direct and intimate contact with humans, companion dogs and cats are considered direct sentinels of vector-borne human pathogens. However, limited information is currently available regarding canine and feline zoonotic pathogens in China. This study detected canine and feline vector-borne human pathogens to better understand the potential risk to humans.

Methods

Blood samples were collected from 275 domestic companion animals (117 dogs and 158 cats) living in Tianjin city, China, and the presence of DNA from Anaplasma, Babesia, Bartonella, and Rickettsia was detected by semi-nested polymerase chain reaction (PCR). The PCR products of the expected size were sequenced, and these newly generated sequences were subjected to BLASTN, nucleotide identity, and phylogenetic analyses.

Results

A total of 24 blood samples tested positive for vector-borne pathogens in companion dogs and cats in Tianjin city, China, with a relatively low positive rate of 8.7%. Specifically, seven human pathogens, including Rickettsia raoultii, Candidatus Rickettsia jingxinensis, Rickettsia sibirica, Rickettsia felis, Babesia venatorum, Bartonella tribocorum, and Bartonella Henselae, were identified. In addition, Anaplasma ovis with zoonotic potential and Candidatus A. cinensis were detected.

Conclusion

Our results indicate substantial genetic diversity in the vector-borne human pathogens circulating in companion dogs and cats. Interventions based on “One Health” should be taken to reduce the potential risks of contracting infection from companion dogs and cats in Tianjin, China.
Phytochemical and Gene Network Analysis Elucidating the Key Genes Involved in the Biosynthesis of Gomisin J in Schisandra sphenanthera
Authors: Wu Bolin , Peng Jiqing , Fu Hanyu , Shao Fengxia , Sheng Song , Wang Sen
Published: March 2024

The biosynthesis and distribution of lignans in medicinal plants, particularly in Schisandra sphenanthera, hold significant pharmacological importance. This study bridges the knowledge gap in understanding the tissue-specific biosynthesis and distribution of these compounds, with a focus on Gomisin J. Our phytochemical analysis revealed a distinct accumulation pattern of Gomisin J, predominantly in the roots, contrasting with the distribution of Pregomisin and Dihydroguaiaretic acid. This finding highlights the roots’ unique role in lignan storage and biosynthesis. Further, differential gene expression analysis across various tissues illuminated the biosynthetic pathways and regulatory mechanisms of these lignans. Utilizing Weighted Gene Co-expression Network Analysis (WGCNA), we identified the MEtan module as a key player, strongly correlated with Gomisin J levels. This module’s in-depth examination revealed the crucial involvement of four cytochrome P450 (CYP) enzymes and eight transcription factors. Notably, the CYP genes DN6828 and DN2874-i3 exhibited up-regulation in roots across both male and female plants, while DN51746 was specifically up-regulated in male roots, indicating a potential gender-specific aspect in Gomisin J biosynthesis. Comparative analysis with functionally characterized CYP71A homologs suggests these CYP genes might be involved in distinct biosynthetic pathways, including terpenoids, alkaloids, and phenylpropanoids, and potentially in lignan biosynthesis. This hypothesis, supported by their more than 55% identity with CYP71As and strong correlation with Gomisin J concentration, opens avenues for novel discoveries in lignan biosynthesis, pending further functional characterization. Our research provides a comprehensive understanding of the genetic and metabolic mechanisms underlying the tissue-specific distribution of lignans in Schisandra sphenanthera, offering valuable insights for their pharmacological exploitation.
Enhancing Chinese Cabbage Production and Quality through IoT-Based Smart Farming in NFT-Hydroponics
Authors: Promwee Athakorn , Nijibulat Sukimplee , Nguyen Hien Huu
Published: March 2024

The rising adoption of agricultural technologies such as the Internet of Things (IoT) or “smart farming” aims to boost crop production in terms of both quantity and quality. This study compares the benefits of a smart farm employing an IoT-based hydroponic system with those of a conventional hydroponic farm, using Chinese cabbage (Brassica pekinensis L.) as the experimental crop. Our primary objective was to automate environmental monitoring, achieving pH level and electrical conductivity (EC) maintenance through smartphone or computer interfaces for nutrient and acid–base solution adjustments. Additionally, we evaluated plant growth and crop quality, finding superior results with the smart hydroponic system. On average, there were substantial increases in various parameters, including total fresh weight (27.14%), total dry weight (48.90%), plant height (11.14%), stem diameter (32.89%), leaf area (94.30%), leaf width (32.36%), leaf length (38.12%), and chlorophyll content (22.73%). Nitrate accumulation in the edible parts of Chinese cabbage remained within safe limits for both systems, reflecting careful nutrient management. These findings highlight the potential of IoT-based technology in enhancing productivity and quality in hydroponic farming, marking a significant step towards revolutionizing traditional agricultural practices for more efficient crop production systems.
Impact of a Dexmedetomidine Intravenous Infusion in Septic Dogs: Preliminary Study
Authors: Di Franco Chiara , Boysen Søren , Vannozzi Iacopo , Briganti Angela
Published: March 2024

The purpose of this study was to determine if a continuous rate infusion (CRI) of dexmedetomidine decreases vasopressor requirements in septic dogs undergoing surgery. Vital parameters, sequential organ failure assessment (SOFA) score, vasopressor requirement, and 28-day mortality were recorded. Dogs were randomly divided into two groups: a dexmedetomidine (DEX) (1 mcg/kg/h) group and a control group (NaCl), which received an equivalent CRI of NaCl. Dogs were premedicated with fentanyl 5 mcg/kg IV, induced with propofol, and maintained with sevoflurane and a variable rate fentanyl infusion. DEX or NaCl infusions were started 10 min prior to induction. Fluid-responsive hypotensive patients received repeated Ringer’s lactate boluses (2 mL/kg) until stable or they were no longer fluid-responsive. Patients that remained hypotensive following fluid boluses received norepinephrine at a starting dose of 0.05 mcg/kg/min, with increases of 0.05 mcg/kg/min. Rescue adrenaline boluses were administered (0.001 mg/kg) if normotension was not achieved within 30 min of starting norepinephrine. The NaCl group received a significantly higher dose of norepinephrine (0.8, 0.4–2 mcg/kg/min) than the DEX group (0.12, 0–0.86 mcg/kg/min). Mortality was statistically lower in the DEX group (1/10) vs. the NaCl group (5/6). Results of this study suggest that a 1 mcg/kg/h CRI of dexmedetomidine decreases the demand for intraoperative vasopressors and may improve survival in septic dogs.
Spatial Variations of Deformation along a Strike-Slip Fault: A Case Study of Xianshuihe Fault Zone, Southwest China
Authors: Li Jinxuan , Guo Songfeng , Qi Shengwen , Wei Qianhui , Zheng Bowen , Zou Yu , Li Yongchao , Zhang Yaguo , Lu Xiao
Published: March 2024

The distribution of damage zones around a fault has long been regarded as a frontier and hot spot in the field of geoscience but is still not fully understood. In this study, we conducted field investigations and tests around the Xianshuihe fault zone (XSHF), a left-lateral strike-slip fault with a length of about 400 km located in the eastern margin of the Tibetan Plateau. The results reveal that the fracture frequency and rock strength parameters present a spatially asymmetric distribution along the fault and have a negative power-law correlation with the distance from the fault. The widths of the damage zones are approximately 20.8 km and 17.1 km in the southwest and northeast directions, respectively. Combined with the previous studies, we presented a negative power-law function to depict the correlation between slip displacement and the width of the damage zone and found that the growth rate of damage zone in faults with low displacement is greater than that in those with large displacement. The study demonstrates that the asymmetric distribution of the damage zone surrounding the XSHF is mainly due to the stress redistribution in different damage zones stemming from the left echelon and different activity rates of the blocks on both sides of the XSHF.
Zero-Dimensional Modeling of the Rate of Injection with a Diesel Common Rail System Using Single-Hole Nozzles with Neat Low-Carbon Fuels
Authors: Rojas-Reinoso Vicente , Mata Carmen , Soriano Jose Antonio , Armas Octavio
Published: March 2024

This paper presents a fuel injection rate predictive model based on zero-dimensional correlations from experimental results. This model estimates the fuel injection rate behavior with varying parameters such as fuel injection pressure-injector energizing, the injection nozzle geometrical characteristics, and fuel viscosity. The model approach was carried out with diesel fuel. Then, the model was applied to the use of two alternative low-carbon fuels without diesel. An experimental methodology was used under controlled conditions, employing an injection rate indicator to measure the injection parameters in real time. The setup was carried out on a pump test bench using a common rail injection system. The results show that the model can be adapted to different injection conditions and fuels.
Identification of Industrial Heat Source Production Areas Based on SDGSAT-1 Thermal Infrared Imager
Authors: Wang Dacheng , Xie Yanmei , Ma Caihong , Zhao Yindi , Yan Dongmei , Chen Hongyu , Fu Bihong , Wan Guangtong , Hou Xiaolin
Published: March 2024

Industrial heat sources (IHSs) are key contributors to anthropogenic heat, air pollution, and carbon emissions. Accurately and automatically detecting their production areas (IHSPAs) on a large scale is vital for environmental monitoring and decision making, yet this is challenged by the lack of high-resolution thermal data. Sustainable Development Science Satellite 1 (SDGSAT-1) thermal infrared spectrometer (TIS) data with the highest resolution (30 m) in the civilian field and a three-band advantage were first introduced to detect IHSPAs. In this study, an IHSPA identification model using multi-features extracted from SDGSAT-1 TIS and Landsat OLI data and support vector machine (SVM) was proposed. First, three brightness temperatures and four thermal radiation indices using SDGSAT-1 TIS and Landsat OLI data were designed to enlarge the temperature difference between IHSPAs and the background. Then, 10 features combined with three indices from Landsat OLI images with the same spatial resolution (30 m) and stable data were extracted. Second, an IHSPA identification model based on SVM and multi-feature extraction was constructed to identify IHSPAs. Finally, the IHS objects were manually delineated and verified using the identified IHSPAs and Google Earth images. Some conclusions were obtained from different comparisons in Wuhai, China: (1) IHSPA identification based on SVM using thermal and optical features can detect IHSPAs and obtain the best results compared with different features and identification models. (2) The importance of using thermal features from the SDGSAT-1 TIS to detect IHSPAs was demonstrated by different importance analysis methods. (3) Our proposed method can detect more IHSs, with greater spatial coverage and smaller areas, compared with the methods of Ma and Liu. This new way to detect IHSPAs can obtain higher-spatial-resolution emissions of IHSs on a large scale and help decision makers target environmental monitoring, management, and decision making in industrial plant processing.
Effect of MXene Nanosheet Sticking on Supercapacitor Device Performance
Authors: Aleksandrova Mariya , Kurtev Nikolay , Pandiev Ivailo
Published: March 2024

Supercapacitors have garnered significant interest in recent years due to their high power density, rapid charge/discharge rates, and long cycle life. MXenes, a family of two-dimensional (2D) transition metal carbides/nitrides, have emerged as promising electrode materials for supercapacitors. However, one major challenge associated with incorporating MXenes in supercapacitor structures is the occurrence of sticking, wherein individual MXene flakes agglomerate, leading to reduced electrode performance. This review paper discusses various causes of sticking and approaches to preventing it, offering insights into the design and development of high-performance MXene-based supercapacitors. The morphology and size of MXene flakes, flake surface chemistry, thickness, surface area/volume ratio, electrode processing techniques (including solvent selection, additives incorporation, and deposition technology), and environmental factors were shown to be the basic factors resulting in sticking of MXene sheets. Among the strategies to mitigate this challenge, surface functionalization and passivation, integration with polymer matrices or carbon nanomaterials, and electrode processing optimization were considered. Possible paths for optimization and future directions of study, such as novel MXene compositions, understanding of interfaces and electrode–electrolyte interactions, development of advanced electrode architectures, and integration of energy storage systems, were assumed.
Effect of the Silanization of Aerosil OX50 in the Properties of Light-Cured Dental Composites
Authors: Díaz-Rodríguez Selena de la Caridad , Tarano-Artigas Oridayma , Herrera-Kao Wilberth , Cauich-Rodríguez Juan Valerio , Cervantes-Uc José Manuel , Rosa-Sainz Ana , La Serna Amisel Almirall , Veranes-Pantoja Yaymarilis
Published: March 2024

In this study, the surface of aerosil OX50 was treated with γ-methacryloxypropyltrimethoxysilane in order to evaluate its effect on light-cured dental composites. Four composites were prepared with Bis-GMA/tetraethylene glycol dimethacrylate/γ-methacryloxypropyltrimethoxysilane, Bis-GMA/tetraethylene glycol dimethacrylate, and a 45% treated or untreated aerosil OX50, using the camphorquinone/N,N-dimethylaminoethyl methacrylate pair as initiator. Evidence of filler silanization was provided by FTIR as a low-intensity absorption at 1707 cm−1 (carbonyl functional group) while thermogravimetric analysis showed a mass loss of approximately 2% associated with the decomposition of γ-methacryloxypropyltrimethoxysilane. The experimental composites studied meet the requirements of the ISO 4049:2019 standard for depth of cure, water sorption, and solubility. The composites are shown to be thermally stable and presented a degree of conversion higher than 70%, being higher than that reported for many commercial composites. Based on the observed properties, the best formulations were those in which the silane is incorporated into the matrix and the filler was previously treated.