bluuebunny/crossref_metadata_2025 huggingface dataset:
A Linguistic Hierarchy Model with Self-Confidence Preference Relations and Its Application in Co-Regulation of Food Safety in China
Authors: Fan Sha , Zhang Hengjie , Tang Huali
Published: August 2019

Linguistic preference relations are widely used by decision makers to elicit their preferences over alternatives in the Group Decision Making (GDM) process. Recent studies have shown that self-confidence, as an important human psychological behavior, has an important influence on decision-making results. However, multiple self-confidence levels of decision makers are seldom considered in the linguistic preference relation. Meanwhile many real-word decision-making problems are analyzed in a hierarchical structure, in which a complicated problem can be divided into several easier comprehended sub-problems. Hence, this paper aims at designing a linguistic hierarchy model with self-confidence preference relation (LHM-SCPR) to discuss complex GDM problems in a hierarchical structure. In the SC-LPR, each element contains two components, the first one is the preference value between pairs of alternatives, and the second one that is defined on a linguistic term set represents decision maker’s self-confidence level associated to the first component. Meanwhile, a nonlinear programming model is proposed to derive individual preference vector from SC-LPR. Then, we apply LHM-SCPR in co-regulation of food safety to present the validity of this method, and find that improving the participation skills regarding co-regulation of food safety is the most pressing task. Finally, detailed comparative analysis and discussion are presented to verify the validity of the proposal.
Geodemographic Area Classification and Association with Mortality: An Ecological Study of Small Areas of Cyprus
Authors: Lamnisos Demetris , Middleton Nicos , Kyprianou Nikoletta , Talias Michael A.
Published: August 2019

Geographical investigations are a core function of public health monitoring, providing the foundation for resource allocation and policies for reducing health inequalities. The aim of this study was to develop geodemographic area classification based on several area-level indicators and to explore the extent of geographical inequalities in mortality. A series of 19 area-level socioeconomic indicators were used from the 2011 national population census. After normalization and standardization of the geographically smoothed indicators, the k-means cluster algorithm was implemented to classify communities into groups based on similar characteristics. The association between geodemographic area classification and the spatial distribution of mortality was estimated in Poisson log-linear spatial models. The k-means algorithm resulted in four distinct clusters of areas. The most characteristic distinction was between the ageing, socially isolated, and resource-scarce rural communities versus metropolitan areas with younger population, higher educational attainment, and professional occupations. By comparison to metropolitan areas, premature mortality appeared to be 44% (95% Credible Intervals [CrI] of Rate Ratio (RR): 1.06–1.91) higher in traditional rural areas and 36% (95% CrI of RR: 1.13–1.62) higher in young semi-rural areas. These findings warrant future epidemiological studies investigating various causes of the urban-rural differences in premature mortality and implementation policies to reduce the mortality gap between urban and rural areas.
Relation between Length of Exposure to Epidural Analgesia during Labour and Birth Mode
Authors: Garcia-Lausin Laura , Perez-Botella Mercedes , Duran Xavier , Mamblona-Vicente Maria Felisa , Gutierrez-Martin Maria Jesus , Gómez de Enterria-Cuesta Eugenia , Escuriet Ramon
Published: August 2019

Objective: To appraise the relationship between the length of exposure to epidural analgesia and the risk of non-spontaneous birth, and to identify additional risk factors. This study is framed within the MidconBirth project. Study design: A multicentre prospective study was conducted between July 2016 and November 2017 in three maternity hospitals in different Spanish regions. The independent variable of the study was the length of exposure to epidural analgesia, and the dependent variable was the type of birth in women with uncomplicated pregnancies. The data was analyzed separately by parity. A multivariate logistic regression was performed. The odds ratios (OR), using 95% confidence intervals (CI) were constructed. Main outcome measures: During the study period, 807 eligible women gave birth. Non-spontaneous births occurred in 29.37% of the sample, and 75.59% received oxytocin for augmentation of labour. The mean exposure length to epidural analgesia when non-spontaneous birth happened was 8.05 for primiparous and 6.32 for multiparous women (5.98 and 3.37 in spontaneous birth, respectively). A logistic regression showed the length of exposure to epidural during labour was the major predictor for non-spontaneous births in primiparous and multiparous women followed by use of oxytocin (multiparous group). Conclusions: The length of exposure to epidural analgesia during labour is associated with non-spontaneous births in our study. It highlights the need for practice change through the development of clinical guidelines, training programs for professionals and the continuity of midwifery care in order to support women to cope with labour pain using less invasive forms of analgesia. Women also need to be provided with evidence-based information.
An Empirical Analysis of Delayed Monthly Bill Payments as an Early Risk Factor of Increased Suicidal Behavior
Authors: Kim Sujin , You Myoungsoon
Published: August 2019

This study examines the potential of delayed monthly bill payments as a predictor of suicidal behavior in South Korea with the highest suicide rate among developed countries. Using the Korea Welfare Panel Study, multivariable logistic regressions examined the association between suicide ideation/attempts and the frequency of late payments on utility bills or National Health Insurance premium during last three years. Confounding factors such as past depression and suicide ideation/attempts history were adjusted for. Among 10,988 individuals, 2.7% reported suicide ideation and 0.11% attempted suicide in the past year, while 7.1% reported they paid late once or more during the last three years. Adults with two or more delayed payment had 2.32 times increased odds of suicidal ideation and 10.99 times increased odds of suicide attempts, compared to having no late payments. Adjusting for other socio-economic characteristics rarely changed the relationship between late payments and suicide ideation/attempts (for suicidal ideation, OR = 2.11; for suicide attempts: OR = 7.44), suggesting the independent effect of late payments on suicide behavior. With these findings, it can be suggested that late payment is an important factor, preemptively signaling suicide behavior with serious consequences in health and life.
Association between Anemia and Cognitive Impairment among Elderly Patients with Heart Failure
Authors: Kim Eun Young , Son Youn-Jung
Published: August 2019

Cognitive impairment is more prevalent in heart failure (HF) patients. Anemia can influence cognitive ability and is likely more prevalent in elderly patients with HF. However, there are limited data on the association of anemia with cognitive impairment in elderly HF patients. This study aimed to identify the association between anemia and cognitive impairment in elderly HF patients. This secondary data analysis included 181 patients aged 60 years or older with HF. Patients were categorized into an anemic or non-anemic group based on World Health Organization (WHO) criteria. We assessed the cognitive function using the Modified Mini-Mental State (3MS) at the time of enrollment. The prevalence of anemia and cognitive impairment in older patients with HF was the same at 35.4%. The main finding of the multiple logistic regression indicated that compared to a non-anemic status, anemia increased the risk of cognitive impairment (odds ratio (OR) = 4.268, 95% confidence interval (CI) = 1.898–9.593, p < 0.001). Healthcare providers should recognize the value of the significance of early assessment of anemic status and cognitive function following HF. A prospective cohort study should identify the pathway of the association between anemia and incidence of cognitive impairment.
Effect of Land Expropriation on Land-Lost Farmers’ Health: Empirical Evidence from Rural China
Authors: Wang Yuxin , Li Wenlong , Xiong Jinping , Li Ying , Wu Huaqing
Published: August 2019

With rapid urbanization and industry development, China has witnessed substantial land acquisition. Using the rural household survey data, this paper examines the impact of land expropriation on land-lost farmers’ self-reported health with the ordered probit model and investigates the possible mechanisms. The results show that the land expropriation puts higher health risks over those land-lost farmers and the health status of land-lost farmers is significantly worse than that of those with land. Land expropriation has a negative impact on the land-lost farmer’s health through income effects and psychological effects. The health status of land-lost farmers can be enhanced through amending current land requisition policies, increasing the amount of compensation, improving the earning capacity of land-lost farmers and strengthening mental health education.
Understanding the Mechanisms of Resistance in EGFR-Positive NSCLC: From Tissue to Liquid Biopsy to Guide Treatment Strategy
Authors: Del Re Marzia , Crucitta Stefania , Gianfilippo Giulia , Passaro Antonio , Petrini Iacopo , Restante Giuliana , Michelucci Angela , Fogli Stefano , de Marinis Filippo , Porta Camillo , Chella Antonio , Danesi Romano
Published: August 2019

Liquid biopsy has emerged as an alternative source of nucleic acids for the management of Epidermal Growth Factor Receptor (EGFR)-mutant non-Small Cell Lung Cancer (NSCLC). The use of circulating cell-free DNA (cfDNA) has been recently introduced in clinical practice, resulting in the improvement of the identification of druggable EGFR mutations for the diagnosis and monitoring of response to targeted therapy. EGFR-dependent (T790M and C797S mutations) and independent (Mesenchymal Epithelial Transition [MET] gene amplification, Kirsten Rat Sarcoma [KRAS], Phosphatidyl-Inositol 4,5-bisphosphate 3-Kinase Catalytic subunit Alpha isoform [PI3KCA], and RAF murine sarcoma viral oncogene homolog B1 [BRAF] gene mutations) mechanisms of resistance to EGFR tyrosine kinase inhibitors (TKIs) have been evaluated in plasma samples from NSCLC patients using highly sensitive methods (i.e., digital droplet PCR, Next Generation Sequencing), allowing for the switch to other therapies. Therefore, liquid biopsy is a non-invasive method able to detect the molecular dynamic changes that occur under the pressure of treatment, and to capture tumor heterogeneity more efficiently than is allowed by tissue biopsy. This review addresses how liquid biopsy may be used to guide the choice of treatment strategy in EGFR-mutant NSCLC.
Expression of Alpha-Enolase (ENO1), Myc Promoter-Binding Protein-1 (MBP-1) and Matrix Metalloproteinases (MMP-2 and MMP-9) Reflect the Nature and Aggressiveness of Breast Tumors
Authors: Cancemi Patrizia , Buttacavoli Miriam , Roz Elena , Feo Salvatore
Published: August 2019

Breast cancer is a complex and heterogeneous disease: Several molecular alterations cause cell proliferation and the acquisition of an invasive phenotype. Extracellular matrix (ECM) is considered essential for sustaining tumor growth and matrix metalloproteinases (MMPs) have been identified as drivers of many aspects of the tumor phenotype. Mounting evidence indicates that both α-enolase (ENO1) and Myc promoter-binding protein-1 (MBP-1) also played pivotal roles in tumorigenesis, although as antagonists. ENO1 is involved in cell growth, hypoxia tolerance and autoimmune activities besides its major role in the glycolysis pathway. On the contrary, MBP-1, an alternative product of ENO1, suppresses cell proliferation and the invasive ability of cancer cells. Since an important task in personalized medicine is to discriminate a different subtype of patients with different clinical outcomes including chances of recurrence and metastasis, we investigated the functional relationship between ENO1/MBP-1 expression and MMP-2 and MMP-9 activity levels in both tissues and sera of breast cancer patients. We focused on the clinical relevance of ENO1 and MMPs (MMP-2 and MMP-9) overexpression in breast cancer tissues: The association between the higher ENO1, MMP-2 and MMP-9 expression with a worse prognosis suggest that the elevated ENO1 and MMPs expression are promising biomarkers for breast cancer. A relationship seems to exist between MBP-1 expression and the decrease in the activity levels of MMP-9 in cancer tissues and MMP-2 in sera. Moreover, the sera of breast cancer patients grouped for MBP-1 expression differentially induced, in vitro, cell proliferation and migration. Our findings support the hypothesis of patient’s stratification based on ENO1, MBP-1 and MMPs expression. Elucidating the molecular pathways through which MBP-1 influences MMPs expression and breast cancer regression can lead to the discovery of new management strategies.
Bioactivity and Bactericidal Mechanism of Histidine-Rich β-Hairpin Peptide Against Gram-Negative Bacteria
Authors: Dong Na , Wang Chensi , Zhang Tingting , Zhang Lei , Xue Chenyu , Feng Xinjun , Bi Chongpeng , Shan Anshan
Published: August 2019

Antibacterial peptides (APMs) are a new type of antibacterial substance. The relationship between their structure and function remains indistinct; in particular, there is a lack of a definitive and fixed template for designing new antimicrobial peptides. Previous studies have shown that porcine Protegrin-1 (PG-1) exhibits considerable antimicrobial activity and cytotoxicity. In this study, to reduce cytotoxicity and increase cell selectivity, we designed histidine-rich peptides based on the sequence template RR(XY)2XDPGX(YX)2RR-NH2, where X represents I, W, V, and F. The results showed that the peptides form more β-hairpin structures in a lipid-rich environment that mimics cell membranes. Among them, the antimicrobial peptide HV2 showed strong antibacterial activity against Gram-negative strains and almost no toxicity to normal cells. The results of our analysis of its antibacterial mechanism showed that peptide HV2 acts on the bacterial cell membrane to increase its permeability, resulting in cell membrane disruption and death. Furthermore, peptide HV2 inhibited bacterial movement in a concentration-dependent manner and had a more robust anti-inflammatory effect by inhibiting the production of TNF-α. In summary, peptide HV2 exhibits high bactericidal activity and cell selectivity, making it a promising candidate for future use as an antibiotic.
Protection of Male Rat Offspring against Hypertension Programmed by Prenatal Dexamethasone Administration and Postnatal High-Fat Diet with the Nrf2 Activator Dimethyl Fumarate during Pregnancy
Authors: Hsu Chien-Ning , Lin Yu-Ju , Yu Hong-Ren , Lin I-Chun , Sheen Jiunn-Ming , Huang Li-Tung , Tain You-Lin
Published: August 2019

Hypertension can originate from early-life exposure to oxidative stress. As reported, dimethyl fumarate (DMF) activates nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and protects against oxidative stress damage. We examined whether maternal DMF therapy protects adult offspring against hypertension programmed by prenatal dexamethasone (DEX) and postnatal high-fat (HF) diet exposure. We examined male Sprague Dawley rat offspring at 4 months of age from five groups (n = 11–13/group): control, DEX (0.1mg/kg i.p. from gestational day 16 to 22), HF (D12331 diet from weaning to 16 weeks of age), DEX+HF, and DEX+HF+DMF (50mg/kg/day via gastric gavage for 3 weeks during pregnancy). Maternal DMF therapy prevented male offspring against hypertension programmed by combined DEX and HF exposures. The protective effects of maternal DMF include reduced oxidative stress, decreased plasma asymmetric dimethylarginine (ADMA) levels, downregulated the renin-angiotensin system (i.e. Ren, Agt, Ace, and Agtr1a), increased renal protein levels of certain nutrient-sensing signals, and promoted autophagy. In conclusion, maternal Nrf2 activation by DMF protects male adult offspring against hypertension programmed by combined DEX and HF exposures. Our results cast a new light on the therapeutic potential of targeting Nrf2 signaling pathway as reprogramming strategies to prevent programmed hypertension in children exposed to antenatal corticosteroids and postnatally excessive consumption of fat.
Combination of Fetal Fraction Estimators Based on Fragment Lengths and Fragment Counts in Non-Invasive Prenatal Testing
Authors: Gazdarica Juraj , Hekel Rastislav , Budis Jaroslav , Kucharik Marcel , Duris Frantisek , Radvanszky Jan , Turna Jan , Szemes Tomas
Published: August 2019

The reliability of non-invasive prenatal testing is highly dependent on accurate estimation of fetal fraction. Several methods have been proposed up to date, utilizing different attributes of analyzed genomic material, for example length and genomic location of sequenced DNA fragments. These two sources of information are relatively unrelated, but so far, there have been no published attempts to combine them to get an improved predictor. We collected 2454 single euploid male fetus samples from women undergoing NIPT testing. Fetal fractions were calculated using several proposed predictors and the state-of-the-art SeqFF method. Predictions were compared with the reference Y-based method. We demonstrate that prediction based on length of sequenced DNA fragments may achieve nearly the same precision as the state-of-the-art methods based on their genomic locations. We also show that combination of several sample attributes leads to a predictor that has superior prediction accuracy over any single approach. Finally, appropriate weighting of samples in the training process may achieve higher accuracy for samples with low fetal fraction and so allow more reliability for subsequent testing for genomic aberrations. We propose several improvements in fetal fraction estimation with a special focus on the samples most prone to wrong conclusion.
Cell Interactions in Biliary Diseases: Clues from Pathophysiology and Repair Mechanisms to Foster Early Assessment
Authors: Fabris Luca , Spirli Carlo , Mertens Joachim
Published: August 2019

In modern hepatology, diseases of the biliary epithelium, currently termed cholangiopathies, represent one of the main gaps in knowledge, both on experimental and clinical grounds, though they started to draw attention since the late 80s [...]
Endothelial Ca2+ Signaling, Angiogenesis and Vasculogenesis: Just What It Takes to Make a Blood Vessel
Authors: Moccia Francesco , Negri Sharon , Shekha Mudhir , Faris Pawan , Guerra Germano
Published: August 2019

It has long been known that endothelial Ca2+ signals drive angiogenesis by recruiting multiple Ca2+-sensitive decoders in response to pro-angiogenic cues, such as vascular endothelial growth factor, basic fibroblast growth factor, stromal derived factor-1α and angiopoietins. Recently, it was shown that intracellular Ca2+ signaling also drives vasculogenesis by stimulation proliferation, tube formation and neovessel formation in endothelial progenitor cells. Herein, we survey how growth factors, chemokines and angiogenic modulators use endothelial Ca2+ signaling to regulate angiogenesis and vasculogenesis. The endothelial Ca2+ response to pro-angiogenic cues may adopt different waveforms, ranging from Ca2+ transients or biphasic Ca2+ signals to repetitive Ca2+ oscillations, and is mainly driven by endogenous Ca2+ release through inositol-1,4,5-trisphosphate receptors and by store-operated Ca2+ entry through Orai1 channels. Lysosomal Ca2+ release through nicotinic acid adenine dinucleotide phosphate-gated two-pore channels is, however, emerging as a crucial pro-angiogenic pathway, which sustains intracellular Ca2+ mobilization. Understanding how endothelial Ca2+ signaling regulates angiogenesis and vasculogenesis could shed light on alternative strategies to induce therapeutic angiogenesis or interfere with the aberrant vascularization featuring cancer and intraocular disorders.
New Molecular Markers Involved in Regulation of Ovarian Granulosa Cell Morphogenesis, Development and Differentiation during Short-Term Primary In Vitro Culture—Transcriptomic and Histochemical Study Based on Ovaries and Individual Separated Follicles
Authors: Kulus Magdalena , Sujka-Kordowska Patrycja , Konwerska Aneta , Celichowski Piotr , Kranc Wiesława , Kulus Jakub , Piotrowska-Kempisty Hanna , Antosik Paweł , Bukowska Dorota , Iżycki Dariusz , Bruska Małgorzata , Zabel Maciej , Nowicki Michał , Kempisty Bartosz
Published: August 2019

Nowadays, science has a lot of knowledge about the physiology of ovarian processes, especially folliculogenesis, hormone production and ovulation. However, the molecular basis for these processes remains largely undiscovered. The cell layer surrounding the growing oocyte—granulosa cells—are characterized by high physiological capabilities (e.g., proliferation, differentiation) and potential for growth in primary cultures, which predisposes them for analysis in the context of possible application of their cultures in advanced methods of assisted reproduction. In this study, we have used standard molecular approaches to analyze markers of these processes in primarily in vitro cultured porcine granulosa, subjected to conditions usually applied to cultures of similar cells. The material for our research came from commercially slaughtered pigs. The cells were obtained by enzymatic digestion of tissues and in vitro culture in appropriate conditions. The obtained genetic material (RNA) was collected at specific time intervals (0 h—before culture; reference, 48, 98, 144 h) and then analyzed using expression microarrays. Genes that showed a fold change greater than |2| and an adjusted p value lower than 0.05 were described as differentially expressed. Three groups of genes: “Cell morphogenesis”, “cell differentiation” and “cell development” were analyzed. From 265 differently expressed genes that belong to chosen ontology groups we have selected DAPL1, CXCL10, NEBL, IHH, TGFBR3, SCUBE1, DAB1, ITM2A, MCOLN3, IGF1 which are most downregulated and PDPN, CAV1, TMOD1, TAGLN, IGFBP5, ITGB3, LAMB1, FN1, ITGA2, POSTN genes whose expression is upregulated through the time of culture, on which we focused in downstream analysis. The results were also validated using RT-qPCR. The aim of our work was to conduct primary in vitro culture of granulosa cells, as well as to analyze the expression of gene groups in relation to the proliferation of follicular granulosa cells in the model of primary culture in real time. This knowledge should provide us with a molecular insight into the processes occurring during the in vitro cultures of porcine granulosa cells, serving as a basic molecular entry on the extent of the loss of their physiological properties, as well as gain of new, culture-specific traits.
Derailed Ceramide Metabolism in Atopic Dermatitis (AD): A Causal Starting Point for a Personalized (Basic) Therapy
Authors: Blaess Markus , Deigner Hans-Peter
Published: August 2019

Active rebuilding, stabilizing, and maintaining the lipid barrier of the skin is an encouraging disease management and care concept for dry skin, atopic dermatitis (eczema, neurodermatitis), and psoriasis. For decades, corticosteroids have been the mainstay of topical therapy for atopic dermatitis; however, innovations within the scope of basic therapy are rare. In (extremely) dry, irritated, or inflammatory skin, as well as in lesions, an altered (sphingo)lipid profile is present. Recovery of a balanced (sphingo)lipid profile is a promising target for topical and personalized treatment and prophylaxis. New approaches for adults and small children are still lacking. With an ingenious combination of commonly used active ingredients, it is possible to restore and reinforce the dermal lipid barrier and maintain refractivity. Lysosomes and ceramide de novo synthesis play a key role in attenuation of the dermal lipid barrier. Linoleic acid in combination with amitriptyline in topical medication offers the possibility to relieve patients affected by dry and itchy skin, mild to moderate atopic dermatitis lesions, and eczemas without the commonly occurring serious adverse effects of topical corticosteroids or systemic antibody administration.
Transcriptional Response of Subcutaneous White Adipose Tissue to Acute Cold Exposure in Mice
Authors: Liang Xiaojuan , Pan Jianfei , Cao Chunwei , Zhang Lilan , Zhao Ying , Fan Yiping , Li Kui , Tao Cong , Wang Yanfang
Published: August 2019

Beige adipose tissue has been considered to have potential applications in combating obesity and its related metabolic diseases. However, the mechanisms of acute cold-stimulated beige formation still remain largely unknown. Here, transcriptional analysis of acute cold-stimulated (4 °C for 4 h) subcutaneous white adipose tissue (sWAT) was conducted to determine the molecular signatures that might be involved in beige formation. Histological analysis confirmed the appearance of beige adipocytes in acute cold-treated sWAT. The RNA-sequencing data revealed that 714 genes were differentially expressed (p-value < 0.05 and fold change > 2), in which 221 genes were upregulated and 493 genes were downregulated. Gene Ontology (GO) analyses showed that the upregulated genes were enriched in the GO terms related to lipid metabolic process, fatty acid metabolic process, lipid oxidation, fatty acid oxidation, etc. In contrast, downregulated genes were assigned the GO terms of regulation of immune response, regulation of response to stimulus, defense response, etc. The expressions of some browning candidate genes were validated in cold-treated sWAT and 3T3-L1 cell browning differentiation. In summary, our results illustrated the transcriptional response of sWAT to acute cold exposure and identified the genes, including Acad11, Cyp2e1, Plin5, and Pdk2, involved in beige adipocyte formation in mice.
Cadmium and Plant Development: An Agony from Seed to Seed
Authors: Huybrechts Michiel , Cuypers Ann , Deckers Jana , Iven Verena , Vandionant Stéphanie , Jozefczak Marijke , Hendrix Sophie
Published: August 2019

Anthropogenic pollution of agricultural soils with cadmium (Cd) should receive adequate attention as Cd accumulation in crops endangers human health. When Cd is present in the soil, plants are exposed to it throughout their entire life cycle. As it is a non-essential element, no specific Cd uptake mechanisms are present. Therefore, Cd enters the plant through transporters for essential elements and consequently disturbs plant growth and development. In this review, we will focus on the effects of Cd on the most important events of a plant’s life cycle covering seed germination, the vegetative phase and the reproduction phase. Within the vegetative phase, the disturbance of the cell cycle by Cd is highlighted with special emphasis on endoreduplication, DNA damage and its relation to cell death. Furthermore, we will discuss the cell wall as an important structure in retaining Cd and the ability of plants to actively modify the cell wall to increase Cd tolerance. As Cd is known to affect concentrations of reactive oxygen species (ROS) and phytohormones, special emphasis is put on the involvement of these compounds in plant developmental processes. Lastly, possible future research areas are put forward and a general conclusion is drawn, revealing that Cd is agonizing for all stages of plant development.
A Novel Peptide Ameliorates LPS-Induced Intestinal Inflammation and Mucosal Barrier Damage via Its Antioxidant and Antiendotoxin Effects
Authors: Zhang Lulu , Wei Xubiao , Zhang Rijun , Si Dayong , Petitte James N. , Ahmad Baseer , Zhang Manyi
Published: August 2019

Intestinal inflammation is an inflammatory disease resulting from immune dysregulation in the gut. It can increase the risk of enteric cancer, which is a common malignancy globally. As a new class of anti-inflammatory agents, native peptides have potential for use in the treatment of several intestinal inflammation conditions; however, their potential cytotoxicity and poor anti-inflammatory activity and stability have prevented their development. Hybridization has been proposed to overcome this problem. Thus, in this study, we designed a hybrid peptide (LL-37-TP5, LTP) by combing the active centre of LL-37 (13–36) with TP5. The half-life and cytotoxicity were tested in vitro, and the hybrid peptide showed a longer half-life and lower cytotoxicity than its parental peptides. We also detected the anti-inflammatory effects and mechanisms of LTP on Lipopolysaccharide (LPS)-induced intestinal inflammation in murine model. The results showed that LTP effectively prevented LPS-induced weight loss, impairment of intestinal tissues, leukocyte infiltration, and histological evidence of inflammation. Additionally, LTP decreased the levels of tumour necrosis factor-alpha, interferon-gamma, and interleukin-6; increased the expression of zonula occludens-1 and occludin; and reduced permeability in the jejunum of LPS-treated mice. Notably, LTP appeared to be more potent than the parental peptides LL-37 and TP5. The anti-inflammatory effects of LTP may be associated with the neutralization of LPS, inhibition of oxidative stress, and inhibition of the NF-κB signalling pathway. The findings of this study suggest that LTP might be an effective therapeutic agent for treating intestinal inflammation.
Effects of Fine-Mesh Exclusion Netting on Pests of Blackberry
Authors: Kuesel Ryan , Scott Hicks Delia , Archer Kendall , Sciligo Amber , Bessin Ricardo , Gonthier David
Published: August 2019

Fine-mesh exclusion netting is a potential alternative to organic and conventional insecticide application to control numerous pests of fruit crops. We tested whether fine-mesh exclusion netting would reduce pest abundance and increase marketable yield compared to organic spinosad insecticide sprays in an organically managed blackberry field. At the completion of flowering, we covered blackberry rows with fine-mesh exclusion netting (ProtekNet) and treated alternating rows with an organic spinosad insecticide (Entrust™). Fine-mesh exclusion reduced green June beetle (Cotinus nitida Linnaeus) and bird presence and marginally reduced Japanese beetle (Popillia japonica Newman) presence on blackberry canes compared to organic spinosad insecticide treatment. Exclusion netting reduced the capture of spotted-wing Drosophila (Drosophila suzukii Matsumara; “SWD”) in baited traps in the fourth week of exclusion and reduced the overall number of SWD adults emerging from harvested blackberry fruits. Marketable yield in the fine-mesh exclusion treatments was two times higher than the organic spinosad insecticide treatment. These results suggest that fine-mesh exclusion netting is a functional pest control alternative to insecticide treatment for organic blackberry production.
Design and Validation of A Modular Instrument to Measure Torque and Energy Consumption in Industrial Operations
Authors: De la Cruz Mary , Gonzalez Ramiro , Gomez Jesus A. , Mendoza Atilano , Ortega Javier A.
Published: August 2019

A modular torque measuring instrument capable of performing tapping torque tests (TTT) according to the ASTM D-5619 standard was designed, developed, and validated. With this new instrument, the performance of different lubricants can be evaluated in terms of frictional torque and energy consumption during tapping processes. This instrument can adapt onto any conventional milling machine or CNC machine and operate under various machining operations such as tapping, drilling, and other processes. To validate the design and performance of this new device, three commercially available lubricants were evaluated. From the three tested conditions, the results showed good repeatability, with consistent results throughout the different tests for each lubricant. The impact of such a proposed instrument ranges from academic use to industrial business use.
The Role of Scale Adjustment in Color Change Evaluation
Authors: Gueli Anna Maria , Pasquale Stefania , Politi Giuseppe , Stella Giuseppe
Published: August 2019

The aim of this research work was to assess the influence of different scale adjustment conditions on both color measurement and color difference quantification and, in particular, to determine the best procedure to follow for a high-reliability protocol. This issue is very important in the Cultural Heritage field and, above all, in color measurement, which is carried out at different times during conservation and restoration campaigns or during archaeological excavations. Color change evaluation, performed by way of spectrophotometric measurements under different scale adjustment procedures on selected samples represented by colored reflectance standards and colored paintings, aimed to obtain results not only on ideal samples (certified standards) but also on real case studies (paintings). The study was conducted by focusing on the coordinates of the CIELAB color space and, in particular, on the calculation of the ΔE*ab quantity. The results show the introduction of nonsystematic variation with different scale adjustment procedures independent of materials and hue.
Does Qigong Practice Have Benefits on the Management of Parkinson’s Disease?
Authors: Moreira António , Matos Luís Carlos , Conceição Ana Maria
Published: August 2019

Parkinson’s disease (PD) is a progressive neurological disorder leading to loss of autonomy and a decline in quality of life. Qigong, a practice rooted in traditional Chinese medicine, has been positively reported on a variety of complaints of chronically ill patients and on gait imbalance in the elderly. PubMed and B-On databases were accessed during March 2018 to carry out an inventory of relevant scientific papers relating PD to Qigong. Fifteen articles were found and analyzed allowing us to highlight that: (1) in addition to medication, Qigong shows potential gains in PD management; (2) there is a stabilizing effect of motor symptoms and positive results in several frequent autonomy symptoms; (3) Qigong is highly accepted by patients, and is a cost-effective treatment that can be self-practiced, improving sleep quality, gait speed, functional mobility and quality of life, thus reducing the risk of falling; (4) Qigong improves muscle hardness, functional ability to walk, hand–eye coordination and balance. Despite the promising results, the limitations and the disparity of experimental designs of the included studies do not allow us to have a conclusive answer to the question whether Qigong benefits the management of PD or not.
Nutrition in Cancer Patients
Authors: Ravasco Paula
Published: August 2019

Background: Despite being recognised that nutritional intervention is essential, nutritional support is not widely accessible to all patients. Given the incidence of nutritional risk and nutrition wasting, and because cachexia management remains a challenge in clinical practice, a multidisciplinary approach with targeted nutrition is vital to improve the quality of care in oncology. Methods: A literature search in PubMed and Cochrane Library was performed from inception until 26 March. The search consisted of terms on: cancer, nutrition, nutritional therapy, malnutrition, cachexia, sarcopenia, survival, nutrients and guidelines. Key words were linked using “OR” as a Boolean function and the results of the four components were combined by utilizing the “AND” Boolean function. Guidelines, clinical trials and observational studies written in English, were selected. Seminal papers were referenced in this article as appropriate. Relevant articles are discussed in this article. Results: Recent literature supports integration of nutrition screening/assessment in cancer care. Body composition assessment is suggested to be determinant for interventions, treatments and outcomes. Nutritional intervention is mandatory as adjuvant to any treatment, as it improves nutrition parameters, body composition, symptoms, quality of life and ultimately survival. Nutrition counselling is the first choice, with/without oral nutritional supplements (ONS). Criteria for escalating nutrition measures include: (1) 50% of intake vs. requirements for more than 1–2 weeks; (2) if it is anticipated that undernourished patients will not eat and/or absorb nutrients for a long period; (3) if the tumour itself impairs oral intake. N-3 fatty acids are promising nutrients, yet clinically they lack trials with homogeneous populations to clarify the identified clinical benefits. Insufficient protein intake is a key feature in cancer; recent guidelines suggest a higher range of protein because of the likely beneficial effects for treatment tolerance and efficacy. Amino acids for counteracting muscle wasting need further research. Vitamins/minerals are recommended in doses close to the recommended dietary allowances and avoid higher doses. Vitamin D deficiency might be relevant in cancer and has been suggested to be needed to optimise protein supplements effectiveness. Conclusions: A proactive assessment of the clinical alterations that occur in cancer is essential for selecting the adequate nutritional intervention with the best possible impact on nutritional status, body composition, treatment efficacy and ultimately reducing complications and improving survival and quality of life.
Acute Limb Ischemia: An Update on Diagnosis and Management
Authors: Olinic Dan-Mircea , Stanek Agata , Tătaru Dan-Alexandru , Homorodean Călin , Olinic Maria
Published: August 2019

This review presents an update on the diagnosis and management of acute limb ischemia (ALI), a severe condition associated with high mortality and amputation rates. A comprehensive spectrum of ALI etiology is presented, with highlights on embolism and in situ thrombosis. The steps for emergency diagnosis are described, emphasizing the role of clinical data and imaging, mainly duplex ultrasound, CT angiography and digital substraction angiography. The different therapeutic techniques are presented, ranging from pharmacological (thrombolysis) to interventional (thromboaspiration, mechanical thrombectomy, and stent implantation) techniques to established surgical revascularization (Fogarty thrombembolectomy, by-pass, endarterectomy, patch angioplasty or combinations) and minor or major amputation of necessity. Postprocedural management, reperfusion injury, compartment syndrome and long-term treatment are also updated.
Meta-Analysis of Differential miRNA Expression after Bariatric Surgery
Authors: Langi Gladys , Szczerbinski Lukasz , Kretowski Adam
Published: August 2019

Bariatric surgery is an efficient treatment for weight loss in obese patients and for resolving obesity comorbidities. However, the mechanisms behind these outcomes are unclear. Recent studies have indicated significant alterations in the transcriptome after surgery, specifically in the differential expression of microRNAs. In order to summarize the recent findings, we conducted a systematic summary of studies comparing microRNA expression levels before and after surgery. We identified 17 animal model and human studies from four databases (Ovid, Scopus, Web of Science, and PubMed) to be enrolled in this meta-analysis. From these studies, we identified 14 miRNAs which had the same direction of modulation of their expression after surgery in at least two studies (downregulated: hsa-miR-93-5p, hsa-miR-106b-5p, hsa-let-7b-5p, hsa-let-7i-5p, hsa-miR-16-5p, hsa-miR-19b-3p, hsa-miR-92a-3p, hsa-miR-222-3p, hsa-miR-142-3p, hsa-miR-140-5p, hsa-miR-155-5p, rno-miR-320-3p; upregulated: hsa-miR-7-5p, hsa-miR-320c). Pathway analysis for these miRNAs was done using database resources (DIANA-TarBase and KEGG pathway database) and their predicted target genes were discussed in relation with obesity and its comorbidities. Discrepancies in study design, such as miRNA source, bariatric surgery type, time of observation after surgery, and miRNA profiling methods, were also discussed.
The Molecular Basis of Human Anophthalmia and Microphthalmia
Authors: Harding Philippa , Moosajee Mariya
Published: August 2019

Human eye development is coordinated through an extensive network of genetic signalling pathways. Disruption of key regulatory genes in the early stages of eye development can result in aborted eye formation, resulting in an absent eye (anophthalmia) or a small underdeveloped eye (microphthalmia) phenotype. Anophthalmia and microphthalmia (AM) are part of the same clinical spectrum and have high genetic heterogeneity, with >90 identified associated genes. By understanding the roles of these genes in development, including their temporal expression, the phenotypic variation associated with AM can be better understood, improving diagnosis and management. This review describes the genetic and structural basis of eye development, focusing on the function of key genes known to be associated with AM. In addition, we highlight some promising avenues of research involving multiomic approaches and disease modelling with induced pluripotent stem cell (iPSC) technology, which will aid in developing novel therapies.
Comparison of HF Radar Fields of Directional Wave Spectra Against In Situ Measurements at Multiple Locations
Authors: Lopez Guiomar , Conley Daniel C.
Published: August 2019

The coastal zone hosts a great number of activities that require knowledge of the spatial characteristics of the wave field, which in coastal seas can be highly heterogeneous. Information of this type can be obtained from HF radars, which offer attractive performance characteristics in terms of temporal and spatial resolution. This paper presents the validation of radar-derived fields of directional wave spectra. These were retrieved from measurements collected with an HF radar system specifically deployed for wave measurement, using an established inversion algorithm. Overall, the algorithm reported accurate estimates of directional spectra, whose main distinctive characteristic was that the spectral energy was typically spread over a slightly broader range of frequencies and directions than in their in situ-measured counterparts. Two errors commonly reported in previous studies, namely the overestimation of wave heights and noise related to short measurement periods, were not observed in our results. The maximum wave height recorded by two in situ devices differed by 30 cm on average from the radar-measured values, and with the exception of the wave spreading, the standard deviations of the radar wave parameters were between 3% and 20% of those obtained with the in situ datasets, indicating the two were similarly grouped around their means. At present, the main drawback of the method is the high quality signal required to perform the inversion. This is in part responsible for a reduced data return, which did not exceed 55% at any grid cell over the eight-month period studied here.
Research on Fractal Characteristics and Energy Dissipation of Concrete Suffered Freeze-Thaw Cycle Action and Impact Loading
Authors: Li Yan , Zhai Yue , Liu Xuyang , Liang Wenbiao
Published: August 2019

In order to study the fractal characteristics and energy dissipation of concrete suffered freeze-thaw cycle actions and impact loading, C35 concrete was taken as the research object in this paper, and freeze-thaw cycle tests were carried out with a freeze-thaw range of −20 °C~20 °C and a freeze-thaw frequency of 0~50 times. The degradation characteristics of concrete material and the variation rules of basic physical parameters under various freeze-thaw cycle conditions were obtained consequently. By using the SHPB (separated Hopkinson pressure bar) test device, impact compression tests of concrete specimens under different freeze-thaw cycle actions were developed, then the process of impact crushing and the mechanism of damage evolution were analyzed. Based on the screening statistical method and the fractal theory, the scale-mass distribution rules and fractal dimension characteristics of crushing blocks are investigated. Furthermore, the absorption energy, fracture energy and block kinetic energy of concrete under different conditions were calculated according to the energy dissipation principle of SHPB test. The relationship between the energy consumption density and the fractal dimension of fragments was established, and the coupling effect mechanism of freeze-thaw cycle action and strain rate effect on the fractal characteristics and energy consumption was revealed additionally. The research results show that the concrete under different freeze-thaw cycle conditions and impact loading speeds has fractal properties from the microscopic damage to the macroscopic fracture. The energy dissipation is intrinsically related to the fractal characteristics, and the energy consumption density increases with the increase of the fractal dimension under a certain freeze-thaw cycle condition. When at a certain loading speed, with the growth of freeze-thaw cycles, the energy consumption density reduces under the same fractal dimension, while the fractal dimension improves under the same energy consumption density.
Development of Smart Composites Based on Doped-TiO2 Nanoparticles with Visible Light Anticancer Properties
Authors: Galata Evdokia , Georgakopoulou Eleni A. , Kassalia Maria-Emmanouela , Papadopoulou-Fermeli Nefeli , Pavlatou Evangelia A.
Published: August 2019

In this study, the synthesis of smart, polymerically embedded titanium dioxide (TiO2) nanoparticles aimed to exhibit photo-induced anticancer properties under visible light irradiation is investigated. The TiO2 nanoparticles were prepared by utilizing the sol gel method with different dopants, including nitrogen (N-doped), iron (Fe-doped), and nitrogen and iron (Fe,N-doped). The dopants were embedded in an interpenetrating (IP) network microgel synthesized by stimuli responsive poly (N-Isopropylacrylamide-co-polyacrylicacid)–pNipam-co-PAA forming composite particles. All the types of produced particles were characterized by X-ray powder diffraction, micro-Raman, Fourier-transform infrared, X-ray photoelectron, ultra-violet-visible spectroscopy, Field Emission Scanning Electron, Transmission Electron microscopy, and Dynamic Light Scattering techniques. The experimental findings indicate that the doped TiO2 nanoparticles were successfully embedded in the microgel. The N-doped TiO2 nano-powders and composite particles exhibit the best photocatalytic degradation of the pollutant methylene blue under visible light irradiation. Similarly, the highly malignant MDA-MB-231 breast cancer epithelial cells were susceptible to the inhibition of cell proliferation at visible light, especially in the presence of N-doped powders and composites, compared to the non-metastatic MCF-7 cells, which were not affected.
A Study on Optimal Machining Conditions and Energy Efficiency in Plasma Assisted Machining of Ti-6Al-4V
Authors: Lee Young-Hun , Lee Choon-Man
Published: August 2019

This research objective was to determine the significant parameters for effective plasma assisted machining (PAM) of Ti-6Al-4V and to derive optimal processing conditions. PAM parameters such as feed rate, spindle speed, and depth of cut have significant effects on its machining characteristic. In this study, the design of experiments (DOE) was used to select optimal machining conditions for PAM. The signal-to-noise (S/N) ratio was analyzed using the Taguchi method and the contributions of the factors were determined using analysis of variance (ANOVA). Finally, the optimal PAM machining conditions were selected using response optimization. In addition, the energy efficiency of conventional machining (CM) and the PAM were compared. The energy efficiency was analyzed by specific cutting energy. The cutting force and surface roughness of PAM decreased by 60.2% and 70.5%, respectively, in optimal PAM machining conditions.