bluuebunny/crossref_metadata_2025 huggingface dataset:
CD8 T cell function and cross-reactivity explored by stepwise increased peptide-HLA versus TCR affinity
Authors: Baumgaertner Petra , Schmidt Julien , Costa-Nunes Carla-Marisa , Bordry Natacha , Guillaume Philippe , Luescher Immanuel , Speiser Daniel E. , Rufer Nathalie , Hebeisen Michael
Published: August 2022

Recruitment and activation of CD8 T cells occur through specific triggering of T cell receptor (TCR) by peptide-bound human leucocyte antigen (HLA) ligands. Within the generated trimeric TCR-peptide:HLA complex, the molecular binding affinities between peptide and HLA, and between TCR and peptide:HLA both impact T cell functional outcomes. However, how their individual and combined effects modulate immunogenicity and overall T cell responsiveness has not been investigated systematically. Here, we established two panels of human tumor peptide variants differing in their affinity to HLA. For precise characterization, we developed the “blue peptide assay”, an upgraded cell-based approach to measure the peptide:HLA affinity. These peptide variants were then used to investigate the cross-reactivity of tumor antigen-specific CD8 T cell clonotypes derived from blood of cancer patients after vaccination with either the native or an affinity-optimized Melan-A/MART-1 epitope, or isolated from tumor infiltrated lymph nodes (TILNs). Vaccines containing the native tumor epitope generated T cells with better functionality, and superior cross-reactivity against potential low affinity escape epitopes, as compared to T cells induced by vaccines containing an HLA affinity-optimized epitope. Comparatively, Melan-A/MART-1-specific TILN cells displayed functional and cross-reactive profiles that were heterogeneous and clonotype-dependent. Finally, we took advantage of a collection of T cells expressing affinity-optimized NY-ESO-1-specific TCRs to interrogate the individual and combined impact of peptide:HLA and TCR-pHLA affinities on overall CD8 T cell responses. We found profound and distinct effects of both biophysical parameters, with additive contributions and absence of hierarchical dominance. Altogether, the biological impact of peptide:HLA and TCR-pHLA affinities on T cell responses was carefully dissected in two antigenic systems, frequently targeted in human cancer immunotherapy. Our technology and stepwise comparison open new insights into the rational design and selection of vaccine-associated tumor-specific epitopes and highlight the functional and cross-reactivity profiles that endow T cells with best tumor control capacity.
Loliginid paralarvae from the Southeastern Gulf of Mexico: Abundance, distribution, and genetic structure
Authors: Guarneros-Narváez Paulina V. , Rodríguez-Canul Rossanna , De Silva-Dávila Roxana , Zamora-Briseño Jesús Alejandro , Améndola-Pimenta Monica , Souza Alejandro José , Ordoñez Uriel , Velázquez-Abunader Iván
Published: August 2022

Global commercial catches of squid have increased greatly in the last few years. However, approximately a quarter of the squid catches are still unidentified. In the southeastern Gulf of Mexico (SGoM), the squid catches are not recorded most of the time and are unidentified. This lack of knowledge limits the evaluation of the populations and prevents the establishment of conservation strategies. In this study, we used morphological and molecular (DNA barcoding– cytochrome c oxidase subunit I gene) identification tools to analyze the species composition of the family Loliginidae based on their paralarvae (PL), their abundance distribution by size class, and their genetic structure on the Yucatan Shelf, SGoM. A total of 134 PL were also collected from three oceanographic cruises held in 2015, 2016, and 2018. A total of 56 adults were collected from three ports of Yucatan. Both adults and PL were identified as Doryteuthis pleii (Blainville, 1823). The highest abundances of PL were detected from the West and the Central zones of the Yucatan Shelf at 50- and 15-m depth isobaths at 163 and 21 km from the coastline, respectively. The abundance was higher (316 PL•1,000 m-3) in the early summer (June 2018), medium (213 PL•1,000 m-3) at the end of summer and early autumn, and very low (24 PL•1,000 m-3) in late autumn. A high haplotype and nucleotide diversity (Hd= 0.77; π= 0.002) with no structured population (Fst < 0) was also found, suggesting a continuous gene flow throughout the Yucatan Shelf. This information establishes the basis for a future comprehensive understanding of their biological cycle and population dynamics.
Automated detection of coastal upwelling in the Western Indian Ocean: Towards an operational “Upwelling Watch” system
Authors: Hammond Matthew Lee , Jebri Fatma , Srokosz Meric , Popova Ekaterina
Published: August 2022

Coastal upwelling is an oceanographic process that brings cold, nutrient-rich waters to the ocean surface from depth. These nutrient-rich waters help drive primary productivity which forms the foundation of ecological systems and the fisheries dependent on them. Although coastal upwelling systems of the Western Indian Ocean (WIO) are seasonal (i.e., only present for part of the year) with large variability driving strong fluctuations in fish catch, they sustain food security and livelihoods for millions of people via small-scale (subsistence and artisanal) fisheries. Due to the socio-economic importance of these systems, an "Upwelling Watch" analysis is proposed, for producing updates/alerts on upwelling presence and extremes. We propose a methodology for the detection of coastal upwelling using remotely-sensed daily chlorophyll-a and Sea Surface Temperature (SST) data. An unsupervised machine learning approach, K-means clustering, is used to detect upwelling areas off the Somali coast (WIO), where the Somali upwelling – regarded as the largest in the WIO and the fifth most important upwelling system globally – takes place. This automatic detection approach successfully delineates the upwelling core and surrounds, as well as non-upwelling ocean regions. The technique is shown to be robust with accurate classification of out-of-sample data (i.e., data not used for training the detection model). Once upwelling regions have been identified, the classification of extreme upwelling events was performed using confidence intervals derived from the full remote sensing record. This work has shown promise within the Somali upwelling system with aims to expand it to the rest of the WIO upwellings. This upwelling detection and classification method can aid fisheries management and also provide broader scientific insights into the functioning of these important oceanographic features.
Energy Harvesting and Thermal Management System in Aerospace
Authors: Jiang Fan , Zhao Shiyu , Wang Lei , Yu Shanmeng , Guan Hongyu , Liu Jinguo
Published: August 2022

Energy harvesting and thermal management in spacecraft refer to the adjustment, distribution, and comprehensive use of uniform adjustment, allocation, and integrated use of the spacecraft and subsystems, which have the advantages of reducing energy consumption and improving the overall performance of the spacecraft. The study is first briefly elaborated on the research progress of energy harvesting and thermal management, and then introduces the key thermal control technology of thermal management, mainly thermal bus systems, heat pump technology, and passive thermal control technology. Finally, the future development direction and research focus of thermal management are proposed, which lays a foundation for the future development of energy harvesting and thermal management technology.
Effect of sodium alginate on the properties of microbial repair materials and its repair effect on mortar crack
Authors: Rong Hui , Hu Kaiyue , Ma Guowei , Zheng Xinguo
Published: August 2022

In recent years, microbial repair materials have received widespread attention for their advantages such as stable products and good compatibility with cementitious materials. However, the current construction process of microbial repair materials is mainly by immersion, which limits the more widespread application. Therefore, this study proposed a coating construction process with sodium alginate as the carrier. The effects of sodium alginate concentration and dosing on the fundamental properties of microbial repair materials (CaCO3 content, mechanical properties, water absorption ratio, and heating shrinkage rate) and their effects on mortar repair effects (macroscopic morphology and surface water absorption ratio) were investigated. The repair effects were further verified by combining thermogravimetric analysis of repair products and scanning electron microscopy. The results showed that 1) the microbial repair material had better basic properties when sodium alginate solution with a mass concentration of 1.5% was used, and its solution-to-bacterial solution volume ratios were 8:2, 7:3, and 6:4; 2) using the coating technique, the microorganisms were fixed in the cracks on the mortar surface by using sodium alginate as the carrier. Only twice of repair could generate a mixture of calcium alginate and calcium carbonate crystals in the cracks by close adhesion. The surface water absorption of the repaired specimens was 67% lower than before the repair, which is a significant effect of the repair.
Time-dependent risk of mortality and end-stage kidney disease among patients with granulomatosis with polyangiitis
Authors: Lin Chun-Yu , Chen Hung-An , Chang Tsang-Wei , Hsu Tsai-Ching , Hsu Chung-Yuan , Su Yu-Jih
Published: August 2022

Objective

To describe the time-dependent impact of granulomatosis with polyangiitis (GPA) on the risk of mortality and end-stage kidney disease (ESKD). The results would provide valuable insight regarding the most vulnerable period for patients with GPA.

Methods

We conducted a retrospective cohort study using a nationally representative database in Taiwan. Patients with incident GPA without prior ESKD were identified, and non-GPA control cohorts were selected and matched to GPA cohorts based on sex, age, entry time and comorbidities in a 1:4 ratio. Cox regression model was used to estimate hazard ratios (HR) for mortality and ESKD stratified by the follow-up period.

Results

We identified a total of 142 GPA patients and 568 matched controls. Of those, 52 GPA patients died during follow-up, 48.1% of whom did so within the first 6 months after diagnosis. The 1-, 3-, 5-, and 10-year survival rates of GPA were 78.2, 71.2, 62.6, and 54.7%, respectively. Patients with GPA exhibited the greatest risk of mortality within the first 6 months after follow-up compared with non-GPA cohorts (HR: 21.9, 95% CI: 8.41–57.5). The mortality risk diminished after 1 year and to a marginally significant level during the follow-up period of 5–10 years (HR: 2.71, 95% CI: 0.97–7.62). Ten (7.1%) of the GPA patients experienced ESKD, and these cases occurred exclusively in the first 3 years following diagnosis.

Conclusion

Our findings suggest that physicians should closely monitor the treatment response and complications of patients with GPA in the first critical 6-month period after diagnosis to improve long-term survival outcome.
ROP-like retinopathy in full/near-term newborns: A etiology, risk factors, clinical and genetic characteristics, prognosis and management
Authors: Sun Limei , Yan Wenjia , Huang Li , Li Songshan , Liu Jia , Lu Yamei , Su Manxiang , Li Zhan , Ding Xiaoyan
Published: August 2022

Purpose

Retinopathy of prematurity (ROP) like retinopathy (ROPLR) could occur in full/near-term newborns. The causes and clinical features are still largely elusive. This study focused on the risk factors, clinical and genetic characteristics, treatment and outcome, and prognosis of ROPLR.

Methods

A total of 47 consecutive full/near-term newborns during 2016–2017 with ROPLR were included. The clinical and genetic characteristics, treatment and outcome, prognosis, and potential underlying etiology of ROPLR were were analyzed.

Results

91 eyes of 47 infants were found to have ROPLR. The ROPLR regressed completely in 65.9% and partially in 20.9% of eyes without any interventions. Retinal changes of family exudative vitreoretinopathy (FEVR) were allocated in 12 neonates (group A), perinatal hypoxia-ischemia were categorized in 17 neonates (group B), and the other 18 neonates were categorized in group C. Compared to those in group B/C, infants in group A had significantly more severe retinopathy (stage 4/5, p < 0.001) and more treatments (p < 0.00 risk factor 1).

Conclusions

Perinatal hypoxia-ischemia might be a major risk factor for ROPLR, in which spontaneous regression was common. FEVR, confirmed by positive family findings and genetic testing, might be the second risk factor of ROPLR, in which retinopathy is more severe and treatment is needed.
Impaired tryptophan metabolism in the gastrointestinal tract of patients with critical coronavirus disease 2019
Authors: Yokoyama Yoshihiro , Ichiki Tomoko , Yamakawa Tsukasa , Tsuji Yoshihisa , Kuronuma Koji , Takahashi Satoshi , Narimatsu Eichi , Nakase Hiroshi
Published: August 2022

Introduction

Coronavirus disease 2019 (COVID-19) is still causing a global pandemic. But the mechanism of COVID-19 severity is not well elucidated.

Materials and methods

We conducted two single-center observational studies of patients with COVID-19. In the first study, the enrolled patients were distinguished based on critical vs. non-critical COVID-19. We collected blood samples from the patients at admission to measure markers related to inflammation and thrombosis and stool samples to analyze the fecal microbiome, metabolome, and calprotectin level. In the second study, we collected ileum and colon tissue samples from patients with critical COVID-19 who required colonoscopy due to severe gastrointestinal symptoms and analyzed mucosal gene expression.

Results

A total of 19 blood samples and 10 stool samples were collected. Interleukin (IL)-6 was the only serum inflammatory marker with significantly higher levels in the critical group than in the non-critical group. The fecal calprotectin level in the critical group was significantly higher than that in the non-critical group (P = 0.03), regardless of the presence of gastrointestinal symptoms. Stool metabolomic analysis showed that the level of indole-3-propionic acid, a ligand for aryl hydrocarbon receptor (AhR), was markedly decreased in the critical group compared to that in the non-critical group (P = 0.01). The expression of genes involved in tryptophan metabolism, including ACE2, AHR, CARD9, and IL22, was downregulated in the ileum of critical COVID-19 patients who required a colonoscopy.

Discussion

Critical COVID-19 patients have gastrointestinal inflammation potentially caused by impaired tryptophan metabolism in the small intestine due to decreased expression of genes involved in tryptophan metabolism.
Bioactive Earthworm Peptides Produced by Novel Protease-Producing Bacillus velezensis PM 35 and Its Bioactivities on Liver Cancer Cell Death via Apoptosis, Antioxidant Activity, Protection Against Oxidative Stress, and Immune Cell Activation
Authors: Wasunan Pimphan , Maneewong Chutamas , Daengprok Wichittra , Thirabunyanon Mongkol
Published: August 2022

Earthworms have long been used as traditional medicine. The purposes of this research were to create bioactive peptides from the unique Amynthas arenulus earthworm (PAAEs) and test their potentials on liver cancer bioprophylactic activity, antioxidant, oxidative stress protection, and immune cell activation. This earthworm had a high protein content ratio, at 55.39%. Besides, PM 35 is one out of 58 bacteria isolated from the earthworm carcasses that exhibited the highest protease and yield protein production which was chosen as the protease-producing bacteria to hydrolyze the protein. The genera were identified by 16S rRNA and 16S–23S rRNA comparison and confirmed as Bacillus velezensis PM 35. The response surface methodology was applied to optimize these hydrolysis parameters, i.e., the enzyme/substrate (E/S) concentration ratio [1%–3% (v/v)] and time (1–3 h) of the hydrolyzing earthworm’s proteins. The optimal hydrolyzing conditions were 3% (v/v) of E/S concentration ratio and 3 h of hydrolysis time, which found protein-hydrolysate yield (24.62%) and degree of hydrolysis (85.45%) as the highest. After being challenged in the gastrointestinal tract-resistant model, these PAAEs (MW <3 and 3–5 kDa) induced liver cancer cell (HepG2) death via apoptotic action modes (cell morphological change and DNA fragmentation). The PAAEs (MW <3 kDa) exhibited significant antioxidant activity via DPPH, ABTS, and FRAP with IC50 values of 0.94, 0.44, and 6.34 mg/ml, respectively. The PAAEs (MW < 3 kDa) were non-cytotoxic and protected the mouse fibroblast cells (L929) against oxidative stress. These PAAEs (MW < 3 kDa, 0.2 mg/ml) stimulated the B lymphocytes (122.3%), and T lymphocytes (126.7%) proliferation. This research suggests that PAAEs can be used in a variety of applications, especially in the food and pharmaceutical industries.
Development and evaluation of a rapid RPA/CRISPR-based detection of Francisella tularensis
Authors: Xu Jian-Hao , Kang Lin , Yuan Bing , Feng Zi-Han , Li Shi-Qing , Wang Jing , Wang Ya-Ru , Xin Wen-Wen , Gao Shan , Li Jia-Xin , Sun Yan-Song , Wang Jing-Lin , Yuan Yuan
Published: August 2022

Francisella tularensis is a dangerous pathogen that causes an extremely contagious zoonosis in humans named tularemia. Given its low-dose morbidity, the potential to be fatal, and aerosol spread, it is regarded as a severe threat to public health. The US Centers for Disease Control and Prevention (CDC) has classified it as a category A potential agent for bioterrorism and a Tier 1 Select Agent. Herein, we combined recombinase polymerase amplification (RPA) with CRISPR/Cas12a system to select the F. tularensis target gene (TUL4), creating a two-pronged rapid and ultrasensitive diagnostic method for detecting F. tularensis. The real-time RPA (RT-RPA) assay detected F. tularensis within 10 min at a sensitivity of 5 copies/reaction, F. tularensis genomic DNA of 5 fg, and F. tularensis of 2 × 102 CFU/ml; the RPA-CRISPR/Cas12a assay detects F. tularensis within 40 min at a sensitivity of 0.5 copies/reaction, F. tularensis genomic DNA of 1 fg, and F. tularensis of 2 CFU/ml. Furthermore, the evaluation of specificity showed that both assays were highly specific to F. tularensis. More importantly, in a test of prepared simulated blood and sewage samples, the RT-RPA assay results were consistent with RT-PCR assay results, and the RPA-CRISPR/Cas12a assay could detect a minute amount of F. tularensis genomic DNA (2.5 fg). There was no nonspecific detection with blood samples and sewage samples, giving the tests a high practical application value. For example, in on-site and epidemic areas, the RT-RPA was used for rapid screening and the RPA-CRISPR/Cas12a assay was used for more accurate diagnosis.
Metagenomic profiles of Dermacentor tick pathogens from across Mongolia, using next generation sequencing
Authors: Altantogtokh Doniddemberel , Lilak Abigail A. , Takhampunya Ratree , Sakolvaree Jira , Chanarat Nitima , Matulis Graham , Poole-Smith Betty Katherine , Boldbaatar Bazartseren , Davidson Silas , Hertz Jeffrey , Bolorchimeg Buyandelger , Tsogbadrakh Nyamdorj , Fiorenzano Jodi M. , Lindroth Erica J. , von Fricken Michael E.
Published: August 2022

Tick-borne diseases are a major public health concern in Mongolia. Nomadic pastoralists, which make up ~ 26% of Mongolia’s population, are at an increased risk of both tick bite exposure and economic loss associated with clinical disease in herds. This study sought to further characterize tick-borne pathogens present in Dermacentor ticks (n = 1,773) sampled in 2019 from 15 of Mongolia’s 21 aimags (provinces). The ticks were morphologically identified and sorted into 377 pools which were then screened using Next-Generation Sequencing paired with confirmatory PCR and DNA sequence analysis. Rickettsia spp. were detected in 88.33% of pools, while Anaplasma spp. and Bartonella spp. were detected in 3.18 and 0.79% of pools, respectively. Khentii had the highest infection rate for Rickettsia spp. (76.61%; CI: 34.65–94.79%), while Arkhangai had the highest infection rate for Anaplasma spp. (7.79%; CI:4.04–13.72%). The exclusive detection of Anaplasma spp. in tick pools collected from livestock supports previous work in this area that suggests livestock play a significant role in disease maintenance. The detection of Anaplasma, Bartonella, and Rickettsia demonstrates a heightened risk for infection throughout Mongolia, with this study, to our knowledge, documenting the first detection of Bartonella melophagi in ticks collected in Mongolia. Further research deploying NGS methods is needed to characterize tick-borne pathogens in other endemic tick species found in Mongolia, including Hyalomma asiaticum and Ixodes persulcatus.
Overexpression of pdeR promotes biofilm formation of Paracoccus denitrificans by promoting ATP production and iron acquisition
Authors: Wang Na , Gao Jie , Xiao Shujie , Zhuang Guoqiang
Published: August 2022

Bacterial biofilms are ubiquitous in natural environments and play an essential role in bacteria’s environmental adaptability. Quorum sensing (QS), as the main signaling mechanism bacteria used for cell-to-cell communication, plays a key role in bacterial biofilm formation. However, little is known about the role of QS circuit in the N-transformation type strain, Paracoccus denitrificans, especially for the regulatory protein PdeR. In this study, we found the overexpression of pdeR promoted bacterial aggregation and biofilm formation. Through RNA-seq analysis, we demonstrated that PdeR is a global regulator which could regulate 656 genes expression, involved in multiple metabolic pathways. Combined with transcriptome as well as biochemical experiments, we found the overexpressed pdeR mainly promoted the intracellular degradation of amino acids and fatty acids, as well as siderophore biosynthesis and transportation, thus providing cells enough energy and iron for biofilm development. These results revealed the underlying mechanism for PdeR in biofilm formation of P. denitrificans, adding to our understanding of QS regulation in biofilm development.
Ethyl acetate produced by Hanseniaspora uvarum is a potential biocontrol agent against tomato fruit rot caused by Phytophthora nicotianae
Authors: Liu Ziyu , Tian Junjie , Yan Hao , Li Delong , Wang Xue , Liang Wenxing , Wang Guangyuan
Published: August 2022

In this study, an oomycete strain FQ01 of Phytophthora nicotianae, which could cause destructive postharvest disease, was isolated. At present, chemical fungicides are the main reagents used for controlling Phytophthora diseases. It is necessary to find new control techniques that are environmentally friendly. The biocontrol activity of Hanseniaspora uvarum MP1861 against P. nicotianae FQ01 was therefore investigated. Our results revealed that the volatile organic compounds (VOCs) released by the yeast strain MP1861 could inhibit the development of P. nicotianae FQ01. The major component of the VOCs produced by the yeast strain MP1861 was identified to be ethyl acetate (70.8%). Biocontrol experiments showed that Phytophthora disease in tomato fruit could be reduced by 95.8% after the yeast VOCs treatment. Furthermore, ethyl acetate inhibited the mycelial growth of the oomycete strain FQ01, and damaged the pathogen cell membrane. This paper describes the pioneering utilization of the yeast strain MP1861 for biocontrol of postharvest fruit rot in tomato caused by P. nicotianae.
Machine learning prediction of postoperative unplanned 30-day hospital readmission in older adult
Authors: Li Linji , Wang Linna , Lu Li , Zhu Tao
Published: August 2022

Background: Although unplanned hospital readmission is an important indicator for monitoring the perioperative quality of hospital care, few published studies of hospital readmission have focused on surgical patient populations, especially in the elderly. We aimed to investigate if machine learning approaches can be used to predict postoperative unplanned 30-day hospital readmission in old surgical patients.

Methods: We extracted demographic, comorbidity, laboratory, surgical, and medication data of elderly patients older than 65 who underwent surgeries under general anesthesia in West China Hospital, Sichuan University from July 2019 to February 2021. Different machine learning approaches were performed to evaluate whether unplanned 30-day hospital readmission can be predicted. Model performance was assessed using the following metrics: AUC, accuracy, precision, recall, and F1 score. Calibration of predictions was performed using Brier Score. A feature ablation analysis was performed, and the change in AUC with the removal of each feature was then assessed to determine feature importance.

Results: A total of 10,535 unique surgeries and 10,358 unique surgical elderly patients were included. The overall 30-day unplanned readmission rate was 3.36%. The AUCs of the six machine learning algorithms predicting postoperative 30-day unplanned readmission ranged from 0.6865 to 0.8654. The RF + XGBoost algorithm overall performed the best with an AUC of 0.8654 (95% CI, 0.8484–0.8824), accuracy of 0.9868 (95% CI, 0.9834–0.9902), precision of 0.3960 (95% CI, 0.3854–0.4066), recall of 0.3184 (95% CI, 0.259–0.3778), and F1 score of 0.4909 (95% CI, 0.3907–0.5911). The Brier scores of the six machine learning algorithms predicting postoperative 30-day unplanned readmission ranged from 0.3721 to 0.0464, with RF + XGBoost showing the best calibration capability. The most five important features of RF + XGBoost were operation duration, white blood cell count, BMI, total bilirubin concentration, and blood glucose concentration.

Conclusion: Machine learning algorithms can accurately predict postoperative unplanned 30-day readmission in elderly surgical patients.
Hierarchical multi-class Alzheimer’s disease diagnostic framework using imaging and clinical features
Authors: Qin Yao , Cui Jing , Ge Xiaoyan , Tian Yuling , Han Hongjuan , Fan Zhao , Liu Long , Luo Yanhong , Yu Hongmei
Published: August 2022

Due to the clinical continuum of Alzheimer’s disease (AD), the accuracy of early diagnostic remains unsatisfactory and warrants further research. The objectives of this study were: (1) to develop an effective hierarchical multi-class framework for clinical populations, namely, normal cognition (NC), early mild cognitive impairment (EMCI), late mild cognitive impairment (LMCI), and AD, and (2) to explore the geometric properties of cognition-related anatomical structures in the cerebral cortex. A total of 1,670 participants were enrolled in the Alzheimer’s Disease Neuroimaging Initiative (ADNI) database, comprising 985 participants (314 NC, 208 EMCI, 258 LMCI, and 205 AD) in the model development set and 685 participants (417 NC, 110 EMCI, 83 LMCI, and 75 AD) after 2017 in the temporal validation set. Four cortical geometric properties for 148 anatomical structures were extracted, namely, cortical thickness (CTh), fractal dimension (FD), gyrification index (GI), and sulcus depth (SD). By integrating these imaging features with Mini-Mental State Examination (MMSE) scores at four-time points after the initial visit, we identified an optimal subset of 40 imaging features using the temporally constrained group sparse learning method. The combination of selected imaging features and clinical variables improved the multi-class performance using the AdaBoost algorithm, with overall accuracy rates of 0.877 in the temporal validation set. Clinical Dementia Rating (CDR) was the primary clinical variable associated with AD-related populations. The most discriminative imaging features included the bilateral CTh of the dorsal part of the posterior cingulate gyrus, parahippocampal gyrus (PHG), parahippocampal part of the medial occipito-temporal gyrus, and angular gyrus, the GI of the left inferior segment of the insula circular sulcus, and the CTh and SD of the left superior temporal sulcus (STS). Our hierarchical multi-class framework underscores the utility of combining cognitive variables with imaging features and the reliability of surface-based morphometry, facilitating more accurate early diagnosis of AD in clinical practice.
Sex differences in brain functional connectivity of hippocampus in mild cognitive impairment
Authors: Williamson Jordan , Yabluchanskiy Andriy , Mukli Peter , Wu Dee H. , Sonntag William , Ciro Carrie , Yang Yuan
Published: August 2022

Mild cognitive impairment (MCI) is the prodromal stage of Alzheimer’s Disease (AD). Prior research shows that females are more impacted by MCI than males. On average females have a greater incidence rate of any dementia and current evidence suggests that they suffer greater cognitive deterioration than males in the same disease stage. Recent research has linked these sex differences to neuroimaging markers of brain pathology, such as hippocampal volumes. Specifically, the rate of hippocampal atrophy affects the progression of AD in females more than males. This study was designed to extend our understanding of the sex-related differences in the brain of participants with MCI. Specifically, we investigated the difference in the hippocampal connectivity to different areas of the brain. The Resting State fMRI and T2 MRI of cognitively normal individuals (n = 40, female = 20) and individuals with MCI (n = 40, female = 20) from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) were analyzed using the Functional Connectivity Toolbox (CONN). Our results demonstrate that connectivity of hippocampus to the precuneus cortex and brain stem was significantly stronger in males than in females. These results improve our current understanding of the role of hippocampus-precuneus cortex and hippocampus-brainstem connectivity in sex differences in MCI. Understanding the contribution of impaired functional connectivity sex differences may aid in the development of sex specific precision medicine to manipulate hippocampal-precuneus cortex and hippocampal-brainstem connectivity to decrease the progression of MCI to AD.
Effects of different types of non-cardiac surgical trauma on hippocampus-dependent memory and neuroinflammation
Authors: Lu Bo , Yuan Hui , Mo Lan , Sun Daofan , Liu Rongjun , Zhou Han , Zhai Xiaojie , Wang Ruichun , Chen Junping , Meng Bo
Published: August 2022

Background

Older individuals have been reported to suffer from cognitive disorders after surgery. Various types of surgical trauma have been used to establish postoperative cognitive dysfunction (POCD) animal models in preclinical studies. However, few comparative analyses of these animal models were conducted.

Methods

Tibial surgery, abdominal surgery, and extended abdominal surgery were performed on aged ICR mice to establish POCD models. Behavioral tests included open field, novel object recognition, fear conditioning, and Morris water maze tests. The Z-score methodology was adopted to obtain a comprehensive and integrated memory performance profile. The changes in hippocampal neuroinflammation were analyzed by ELISA, PCR, and immunofluorescence.

Results

In this study, we found that each type of non-cardiac surgical trauma has a different effects on locomotor activity. Tibial and extended abdominal surgeries led to more significant cognitive impairment than abdominal surgery. Inflammatory cytokines peaked on postoperative day 1 and decreased to control levels on days 3 and 7. Hippocampal neuroinflammation indicators between the three surgery types on postoperative day 1 had no statistical differences.

Conclusion

Overall, the type and intensity of non-cardiac surgical trauma can affect cognitive behavioral outcomes and central inflammation. The shortcomings and emerging issues of POCD animal research methods need to be further studied and solved.
The role of mechanobiology on the Schwann cell response: A tissue engineering perspective
Authors: Manganas Phanee , Kavatzikidou Paraskevi , Kordas Antonis , Babaliari Eleftheria , Stratakis Emmanuel , Ranella Anthi
Published: August 2022

Schwann cells (SCs), the glial cells of the peripheral nervous system (PNS), do not only form myelin sheaths thereby insulating the electrical signal propagated by the axons, but also play an essential role in the regeneration of injured axons. SCs are inextricably connected with their extracellular environment and the mechanical stimuli that are received determine their response during development, myelination and injuries. To this end, the mechanobiological response of SCs is being actively researched, as it can determine the suitability of fabricated scaffolds for tissue engineering and regenerative medicine applications. There is growing evidence that SCs are sensitive to changes in the mechanical properties of the surrounding environment (such as the type of material, its elasticity and stiffness), different topographical features provided by the environment, as well as shear stress. In this review, we explore how different mechanical stimuli affect SC behaviour and highlight the importance of exploring many different avenues when designing scaffolds for the repair of PNS injuries.
Research on efficient feature extraction: Improving YOLOv5 backbone for facial expression detection in live streaming scenes
Authors: Li Zongwei , Song Jia , Qiao Kai , Li Chenghai , Zhang Yanhui , Li Zhenyu
Published: August 2022

Facial expressions, whether simple or complex, convey pheromones that can affect others. Plentiful sensory input delivered by marketing anchors' facial expressions to audiences can stimulate consumers' identification and influence decision-making, especially in live streaming media marketing. This paper proposes an efficient feature extraction network based on the YOLOv5 model for detecting anchors' facial expressions. First, a two-step cascade classifier and recycler is established to filter invalid video frames to generate a facial expression dataset of anchors. Second, GhostNet and coordinate attention are fused in YOLOv5 to eliminate latency and improve accuracy. YOLOv5 modified with the proposed efficient feature extraction structure outperforms the original YOLOv5 on our self-built dataset in both speed and accuracy.
Post-ASPECTS based on hyperdensity in NCCT immediately after thrombectomy is an ultra-early predictor of hemorrhagic transformation and prognosis
Authors: Chen Lulu , Xu Ziqi , Zhang Chen , Ji Yachen , Huang Xianjun , Yang Weimin , Zhou Zhiming , Wang Shuiping , Wang Kai , Luo Benyan , Wang Jingye
Published: August 2022

Background and Purpose

Almost half of the patients exhibit futile recanalization after thrombectomy; however, the early postoperative predictors of futile recanalization remain unclear. We analyzed the relationship of post-thrombectomy ASPECTS (Post-ASPECTS) with 90-day prognosis and hemorrhagic transformation (HT).

Methods

We collected data from patients with acute ischemic stroke (AIS) with anterior-circulation large vessel occlusion (ACLVO) who were treated via thrombectomy within 10 h in 3 hospitals. Successful endovascular recanalization was achieved (modified thrombolysis in cerebral ischemia [mTICI] 2b/3). Non-contrast computed tomography (NCCT) examination was performed immediately (within 1 h) after thrombectomy. Post-ASPECTS were scored based on the brain parenchymal hyperdensity in NCCT according to the ASPECTS scoring method. HT was defined according to the ECASS II classification criteria. Linear correlation, logistic regression, and receiver operating characteristic curve analyses were used to determine the influencing factors and best predictive value of 90-day prognosis, 90-day death, and HT.

Results

A total of 231 patients were enrolled. The good prognosis rate, mortality rate, and HT rate were 57.1, 9.5, and 38.3%, respectively. The Post-ASPECTS affected poor prognosis, death, and HT. The best predictive value of Post-ASPECTS for poor prognosis, death, and HT was 7. The specificities of Post-ASPECTS for predicting HT, poor prognosis, and death were 87.6% (AUC, 0.811; P < 0.001), 87.1% (AUC, 0.768; P < 0.001), and 73.7% (AUC, 0.748; P < 0.001), with positive predictive values of 74.2, 75.7, and 21.4%, respectively.

Conclusion

Post-ASPECTS predicted 90-day prognosis, death, and HT with high specificity and high positive predictive value in patients with AIS with ACLVO. Post-ASPECTS may be an ultra-early predictor of prognosis after thrombectomy.
Case report: Onyx embolization of tentorial dural arteriovenous fistula via the meningohypophyseal trunk and medial tentorial artery of Bernasconi-Cassinari
Authors: Hou Kun , Yu Jinlu
Published: August 2022

For tentorial dural arteriovenous fistula (TDAVF), the meningohypophyseal trunk (MHT), and medial tentorial artery (MTA) of Bernasconi-Cassinari are rarely used as transarterial paths to perform the successful endovascular treatment (EVT). We reported a TDAVF mainly fed by the MHT. Onyx-18 casting in the MTA of Bernasconi-Cassinari under the assistance of coil embolization in proximal MHT was performed. The technique was reported in case 1. At the same time, case 2 with a similar TDAVF was chosen as a control. In case 1, a 52-year-old man suffered a cerebellar hemorrhage. A TDAVF was confirmed by computed tomography angiography and digital subtraction angiography. The feeding arteries included the MHT, middle meningeal artery (MMA), and the artery of Wollschlaeger and Wollschlaeger of the superior cerebellar artery. The MHT and MTA of Bernasconi-Cassinari were hypertrophied. First, a Marathon microcatheter was placed in the MTA to wait for Onyx casting, and then an Echelon-10 microcatheter was placed in the proximal MHT trunk with an aneurysmal dilation to perform coiling to prevent Onyx reflux. Then, Onyx casting obliterated the TDAVF. Case 2 was a 75-year-old woman with TDAVF, and the MTA of Bernasconi-Cassinari was the main feeder. First, the TDAVF experienced incomplete EVT with Onyx casting via the MTA under no assistance of coil embolization in the proximal MTA. The second EVT had to be performed via MMA. Then, Onyx casting obliterated the TDAVF. Therefore, for selected TDAVFs with hypertrophied MHT, under the assistance of coil embolization in proximal MHT, Onyx casting via MHT can finish the complete EVT.
Association between blood pressure variability and clinical outcomes after successful recanalization in patients with large vessel occlusion stroke after mechanical thrombectomy
Authors: Lu You , Shen Rui , Lin Wenjian , Zhou Xiaoyu , Hu Jian , Zhang Quanbin
Published: August 2022

Objective

Nearly half of patients who undergo mechanical thrombectomy (MT) do not experience a favorable outcome. The association between blood pressure fluctuation and clinical outcomes after successful MT is controversial. We evaluated the influence of blood pressure variability (BPV) on the clinical outcomes of stroke patients with large vessel occlusion (LVO) who underwent successful recanalization after MT.

Methods

Patients with anterior circulation LVO stroke who underwent successful emergency MT (modified Thrombolysis in Cerebral Infarction, mTICI ≥ 2b) at the Shanghai Tenth People's Hospital of Tongji University from 2017 to 2021 were enrolled. Multivariate logistic models were used to investigate the association between BPV (mean arterial pressure [MAP] assessed using the standard deviation [SD]) and clinical outcomes. The primary outcome was 90-day modified Rankin Scale scores (mRS), and the secondary outcomes were 30-day mortality and symptomatic intracranial hemorrhage (sICH).

Results

A total of 458 patients (56.8% men), with a mean age of 72 ± 1 years, were enrolled. Among them, 207 (45.2%) patients had unfavorable functional outcomes (mRS score 3–6) at 90 days, 61 (13.3%) patients died within 30 days, and 20 (4.4%) patients had sICH. In a fully adjusted model, BPV was associated with a higher risk of a 90-day mRS score of 3–6 (P = 0.04), 30-day mortality (P < 0.01), and sICH (P < 0.01). A significant interaction between MAP SD and rescue futile recanalization treatment was observed (P < 0.01).

Conclusions

Among patients with LVO stroke who underwent successful recanalization, higher BPV was associated with worse functional outcomes, especially in those who underwent rescue treatment.
Case report: Adult-onset neuronal intranuclear inclusion disease with an amyotrophic lateral sclerosis phenotype
Authors: Fujita Masako , Ueno Tatsuya , Miki Yasuo , Arai Akira , Kurotaki Hidekachi , Wakabayashi Koichi , Tomiyama Masahiko
Published: August 2022

Amyotrophic lateral sclerosis (ALS) is one of the differential diagnoses of diseases that occur in adulthood and lead to progressive generalized muscle weakness. Neuronal intranuclear inclusion disease (NIID) is a disease in which histopathologically eosinophilic nuclear inclusion bodies are found in various systems. Both familial and sporadic forms of the disease have been reported. Most cases of sporadic NIID are of the dementia type, in which the main symptom is dementia at the first onset. Familial NIID is more diverse, with the main dominant symptoms being muscle weakness (NIID-M), dementia (NIID-D), and parkinsonism (NIID-P). Furthermore, recently, a GGC-repeat expansion in the Notch 2 N-terminal like C (NOTCH2NLC) gene, which produces a toxic polyglycine-containing protein (uN2CpolyG) in patients with NIID, has been associated with the pathogenesis of ALS. These results suggest that sporadic NIIDs may have more diverse forms. To date, no autopsy cases of NIID patients with an ALS phenotype have been reported. Here, we describe the first autopsy case report of a patient with sporadic NIID who had been clinically diagnosed with ALS. A 65-year-old Japanese man with no family history of neuromuscular disease developed progressive muscle atrophy and weakness in all limbs. The patient was diagnosed with ALS (El Escoriral diagnostic criteria: probable ALS, laboratory-supported ALS). He had no cognitive dysfunction or neuropathies suggestive of NIID. He required respiratory assistance 48 months after onset. He died of pneumonia at the age of 79 years. Postmortem examinations revealed neuronal loss in the spinal anterior horns and motor cortex. In these affected regions, eosinophilic, round neuronal intranuclear inclusions were evident, which were immunopositive for ubiquitin, p62, and uN2CpolyG. No Bunina bodies or TDP-43-positive inclusions were observed in the brain or spinal cord. Our findings suggest that a small proportion of patients with NIID can manifest a clinical phenotype of ALS. Although skin biopsy is commonly used for the clinical diagnosis of NIID, it may also be useful to identify cases of NIID masquerading as ALS.
Reproducing a decision-making network in a virtual visual discrimination task
Authors: Trapani Alessandra , Sheiban Francesco Jamal , Bertone Elisa , Chiosso Serena , Colombo Luca , D'Andrea Matteo , De Santis Francesco , Fati Francesca , Fossati Veronica , Gonzalez Victor , Pedrocchi Alessandra
Published: August 2022

We reproduced a decision-making network model using the neural simulator software neural simulation tool (NEST), and we embedded the spiking neural network in a virtual robotic agent performing a simulated behavioral task. The present work builds upon the concept of replicability in neuroscience, preserving most of the computational properties in the initial model although employing a different software tool. The proposed implementation successfully obtains equivalent results from the original study, reproducing the salient features of the neural processes underlying a binary decision. Furthermore, the resulting network is able to control a robot performing an in silico visual discrimination task, the implementation of which is openly available on the EBRAINS infrastructure through the neuro robotics platform (NRP).
Dietary selenium intake and the risk of kidney stones in adults, an analysis of 2007–2018 National Health and Nutrition Examination Survey, a cross-sectional study
Authors: Liu Minghui , Cui Zhongxiao , Chen Jinbo , Gao Meng , Zhu Zewu , Chen Hequn
Published: August 2022

Purpose

To evaluate the association between dietary selenium intake and the risk of kidney stones in adults.

Materials and methods

We performed a cross-sectional analysis using data from 2007 to 2018 National Health and Nutrition Examination Survey (NHANES). Dietary intake information of 30,184 participants was obtained using first 24-h dietary recall interview, and kidney stones were presented by a standard questionnaire. The quartile analysis, stratified analysis and non-linearity analysis were used to estimate the association between dietary selenium intake and kidney stones after an adjustment for potential confounders.

Results

The multiple logistic regression indicated that the fourth quantile (Q4) of dietary selenium intake had a lower risk of kidney stones than the first quantile (Q1) in Model 3 (OR 0.82, P < 0.05). The stratified analyses indicated there were statistical differences between dietary selenium intake and kidney stones among younger (age < 50) (OR 0.65, P < 0.01), male (OR 0.73, P < 0.01) and overweight/obese (BMI ≥ 25.0) (OR 0.80, P < 0.05) individuals in Model 3. The non-linear relationship was founded between dietary selenium intake and kidney stones in all participants, younger, male and overweight/obese individuals after adjusting for confounding factors.

Conclusion

Our study revealed an inverse relation between the level of dietary selenium intake and the risk of kidney stones for the United States population, especially for younger (age < 50), male and overweight/obese (BMI ≥ 25.0) individuals. The study provides preliminary guidance on dietary selenium intake for the prevention of kidney stones in different populations. Further studies are required to confirm our findings and clarified the biological mechanisms.
The effect of different weight loss strategies to treat non-alcoholic fatty liver disease focusing on fibroblast growth factor 21
Authors: Power Guerra Nicole , Leyens Katharina , Müller Luisa , Brauer David , Janowitz Deborah , Schlick Samin , Pilz Kristin , Grabe Hans J. , Vollmar Brigitte , Kuhla Angela
Published: August 2022

Objective

Obesity, often associated with non-alcoholic fatty liver disease (NAFLD), is characterized by an imbalance between energy expenditure and food intake, which is also reflected by desensitization of fibroblast growth factor 21 (FGF21). FGF21 is strongly influenced, among others, by TNFα, which is known to be upregulated in obesity-induced inflammation. Successful long-term treatments of NAFLD might be dietary modification, exercise, or fasting.

Materials and methods

Whether succeeded NAFLD recovery is linked with improved FGF21 sensitivity and finally reverted FGF21 resistance was the focus of the present study. For this purpose, mice received a high-fat diet (HFD) for 6 months to establish obesity. Afterward, the mice were subjected to three different weight loss interventions, namely, dietary change to low-fat diet (LFD), treadmill training, and/or time-restricted feeding for additional 6 months, whereas one group remained on HFD.

Results

In addition to the expected decrease in NAFLD activity with dietary change, this was also observed in the HFD group with additional time-restricted feeding. There was also an associated decrease in hepatic TNFα and FGF21 expression and an increase in ß-klotho expression, demonstrated mainly by using principal component analysis. Pearson correlation analysis shows that independent of any intervention, TNFα expression decreased with improved NAFLD recovery. This was accompanied with higher FGF21 sensitivity, as expressed by an increase in β-klotho and FGFR1c expression and concomitantly decreased FGF21 levels.

Conclusion

In summary, we conclude that successful NAFLD therapy is associated with a reversion of the TNFα-triggered FGF21-resistant state or desensitization.