bluuebunny/crossref_metadata_2025 huggingface dataset:
Fatigue Behavior of H-Section Piles under Lateral Loads in Cohesive Soil
Authors: Pérez José A. , Ponce-Torres Alberto , Ríos José D. , Sánchez-González Estíbaliz
Published: October 2024

Most structures supporting solar panels are found on thin-walled metal piles partially driven into the ground, optimizing costs and construction time. These pile foundations are subjected to repetitive lateral loads from various external forces, such as wind, which can compromise the integrity of the pile-soil system. Given that the expected operational lifespan of photovoltaic solar plants is generally 20–30 years, predicting their service life under fatigue loads is crucial. This research analyzes the response of H-section piles to lateral fatigue loads in cohesive rigid soils through four field tests, subjected to load cycles of 55%, 72%, and 77% of the static failure load, corresponding to maximum loads of 25 kN, 32 kN, and 35 kN, respectively. Additionally, the effect of load cycles on the degradation of pile-soil adhesion is studied through two pull-out tests following cyclic tests. This study reveals that soil fatigue does not occur under repetitive loads and that soil stiffness remains constant once the cycles causing soil compaction have been overcome. Nevertheless, the accumulated plastic deflection of the soil increases steadily once soil compaction occurs due to cyclic loading. The implications of these results on the fatigue life of photovoltaic solar panel foundations are discussed.
Automatic Planning Method of Construction Schedule under Multi-Dimensional Spatial Resource Constraints
Authors: Wang Luqi , Li Jinrui , Ye Qizhi , Li Yan , Feng Anqi
Published: October 2024

To enhance the application of building information modeling (BIM) in schedule management for engineering projects, improve spatial planning capabilities, and minimize subjective factors in schedule preparation, this paper presents a framework and method for automatic construction schedule planning based on BIM and data-driven approaches under multi-dimensional spatial resource constraints. The method includes data acquisition, construction process reasoning, time parameter calculation, and schedule generation and scheduling. Initially, the construction data from the BIM model and manual input are imported into the reasoning system to establish the logical relationships of the construction process. Construction time parameters and spatial resource requirements are then automatically calculated using MySQL. Subsequently, the spatial layout and resource allocation data exported from the BIM model are compared to assess whether the space meets the project requirements. Finally, a construction schedule that satisfies the space constraints is generated. The method was validated through a case study, demonstrating its effectiveness in automatic schedule planning under human-computer interaction. This study introduces an innovative approach that considers spatial conflicts in the automatic scheduling process, improving planning efficiency and reducing the risk of spatial conflicts on construction sites.
Seismic Behavior of Concrete Beam-Column Joints Reinforced with Steel-Jacketed Grouting
Authors: Yang Xinzhuo , Dong Yiding , Liu Xi , Qiu Tong , Zhou Jian
Published: October 2024

Joints in frame structures often fail before beams and columns in an earthquake and are a key part of reinforcement. In this study, to enhance the seismic performance of concrete frame structures, a steel-jacketed grouting composite reinforcement method is proposed by combining reinforcement technology, steel cladding technology, and eco-efficient materials from grouting technology. This method effectively utilizes the advantages of various materials, avoids major demolition and construction, and reduces waste and resource consumption. In order to verify the feasibility and effectiveness of the reinforcement method, one of the original joint specimens with a scale of 1:3 and one of the reinforced joint specimens were designed and tested. The experiments involved reversed cyclic testing of beam–column to measure its seismic behavior. The seismic performance indexes such as failure characteristics, hysteretic properties, and the energy dissipation capacity of the specimens were analyzed, and the corresponding finite element model was established. The influence of key parameters such as reinforcement range, steel plate thickness, and grout strength on its seismic performance was explored. The research shows that the method can effectively improve the seismic performance of the joints, and seismic performance indexes such as bearing capacity, ductility, and energy consumption of the specimens are significantly improved. The test results of the established finite element model are in good agreement. The variable parameter analysis of the finite element shows that the thickness of the steel plate has little influence on its bearing capacity. With the increase in the reinforcement range of the clad steel and the strength of the grouting material, the bearing capacity of the specimen increases. The research results can provide a reference for the reinforcement of frame structure joints.
Automatic Design and Monitoring of Mass Concrete Based on Information Technology
Authors: Han Daguang , Ying Chunli , Qi Yujun , Liu Qinghao , Wu Rui , Xia Chuanbang
Published: October 2024

Mass concrete construction has the characteristics of large scale, complex technology, high professional requirements, and complex management. In this paper, information technology is introduced into the construction process of mass concrete, aiming to develop a system that integrates the automatic design and visual management of mass concrete construction monitoring schemes to improve its construction efficiency. In this paper, the automatic design of a mass concrete construction monitoring scheme is designed, the information of its data acquisition terminal sensor is extended, and the sensor information model is created based on the IFC framework. Finally, this paper verifies the feasibility and practicability of the automatic design and visual monitoring of the monitoring scheme through the actual case-commercial complex raft foundation. The results show that the method provides a digital and information platform for mass concrete construction, highlighting the advantages of the proposed method and the traditional method.
Study on Innovative Laminated Flooring with Resin-Impregnated Paper
Authors: Zhou Kankan , Cheng Jianhui , Fan Minliang
Published: October 2024

A new type of laminated flooring decorated by resin impregnated paper (LWFWRIP) was designed, with the advantages of low formaldehyde emission, improved flame retardant, and high wear resistance. The structure of this new type of wood flooring is based on the ordinary laminated flooring, followed by a decorative layer of thin wood pieces, and then the transparent improved flame retardant, wear-resistant paper is added to the top. It is found that the hot-pressing temperature is the most significant factor affecting the adhesion of resin impregnated paper. The optimal hot-pressing parameters are selected as the hot-pressing pressure of 3.5 MPa, hot-pressing temperature of 180 °C, and hot-pressing time of 40 s. The new laminated flooring was improved with high flame retardant, high wear-resistant, combined with the conventional advantages of both solid wood composite flooring and reinforced wood flooring. The new laminated flooring decorated by resin impregnated paper has broad application prospects.
Experimental Study on the Behavior of Reinforced Concrete Derailment Containment Provisions under Quasi-Static Loads
Authors: Nguyen Huy Q. , Kim Hoi Jin , Lim Nam-Hyoung , Kang Yun-Suk , Kim Jung J.
Published: October 2024

Derailments pose a significant threat to high-speed rail safety. The development of effective derailment containment provisions (DCPs) that can be installed within a track gauge and withstand impact loads of derailed wheels while controlling the lateral movement of derailed trains is essential. This paper presents an experimental study on the behavior of reinforced concrete (RC) DCP systems under quasi-static loading. Three steel anchors were assessed for their performance and load-bearing capacity in a single-anchor test. Four full-scale DCP system tests were carried out to examine the effects of scenarios of impact load positions at the anchor and mid-span of the DCPs. The crack pattern, failure mechanism, load–displacement relationship, initial stiffness, and absorber energy capacity of the DCP specimens were acquired. The findings reveal that the failure mode of the DCP specimens was predominantly affected by the tension failure of the steel anchors. The load-carrying capacity and performance equivalent of the DCP system under the applied load scenarios significantly exceeded the design load, ranging from 125% to 168%. Also, the initial stiffness of the DCP system remains largely unaffected by the applied load positions, whereas the absorption energy capacity exhibits a contrasting trend.
Comparison between Design Methods for Seismic Retrofit of Reinforced Concrete Frames Using Dissipative Bracing Systems
Authors: Colajanni Piero , Ahmed Muhammad , D’Anna Jennifer
Published: October 2024

Braces equipped with dissipative devices are among the most widespread methods for the seismic strengthening of seismically prone reinforced concrete (RC) frames. It allows for high reductions in seismic vulnerability with inexpensive, quickly executed interventions. They can often be carried out mainly at the exterior, resulting in interruptions of use that are limited both in time and to only small portions of the building. The design methods of dissipative devices are based on the extensive use of pushover analyses (POA). POA is capable of highlighting the structural deficiencies of the building and comparing the performances of design performed according to different methods and sizing criteria. In the present work, with reference to a case study represented by a four-story spatial frame having characteristics representative of design and construction common practice of the 1970s in Southern European countries, the performances of three different design methods were evaluated and compared. The examined procedures differ, including the following: (i) methods for estimating the peak displacement response of the nonlinear systems, namely (i1) the well-known equal displacement rule and (i2) the equivalent (secant) stiffness and damping rule, and (ii) criteria for distributing stiffness and strength of the braces along the height, namely (ii1) the distribution of stiffness and strength proportionally to those of the frame and (ii2) methods that vary the stiffness and strength along the height in order to minimize the eventual irregularity in elevation of the bare frame. The effectiveness of the procedures was checked by both POA and nonlinear response history analysis, the latter performed assuming both unidirectional and bidirectional input. The stiffness was found to increase by about 10 times and the strength between 7.5 to 3.7 times depending on the design method, and reduction in the displacements ranged between 31% and 42% compared to the values of the original frame. The pros and cons of each procedure are summarized, as all procedures are able to provide brace designs that meet the performance requirements set during the design phase.
H3K27me3 Loss in Central Nervous System Tumors: Diagnostic, Prognostic, and Therapeutic Implications
Authors: Angelico Giuseppe , Mazzucchelli Manuel , Attanasio Giulio , Tinnirello Giordana , Farina Jessica , Zanelli Magda , Palicelli Andrea , Bisagni Alessandra , Barbagallo Giuseppe Maria Vincenzo , Certo Francesco , Zizzo Maurizio , Koufopoulos Nektarios , Magro Gaetano , Caltabiano Rosario , Broggi Giuseppe
Published: October 2024

Central nervous system (CNS) tumors represent a formidable clinical challenge due to their molecular complexity and varied prognostic outcomes. This review delves into the pivotal role of the epigenetic marker H3K27me3 in the development and treatment of CNS tumors. H3K27me3, specifically the trimethylation of lysine 27 on the histone H3 protein, plays a crucial role in regulating gene expression and maintaining chromatin architecture (e.g., in X-chromosome inactivation). Notably, a reduction in H3K27me3 levels, frequently tied to mutations in the H3 gene family such as H3F3A and HIST1H3B, is evident in diverse brain tumor variants, including the diffuse midline glioma characterized by the H3K27M mutation and certain pediatric high-grade gliomas. The loss of H3K27me3 has been linked to more aggressive behavior in meningiomas, with the trimethylation loss associated with significantly shorter recurrence-free survival (RFS) among grade 2 meningiomas, albeit not within grade 1 tumors. Pediatric posterior fossa ependymomas characterized by a lowered H3K27me3 and DNA hypomethylation exhibit poor prognosis, underscoring the prognostic significance of these epigenetic alterations in CNS tumors. Comprehending the role of H3K27me3 in CNS tumors is vital for advancing diagnostic tools and therapeutic interventions, with the goal of enhancing patient outcomes and quality of life. This review underscores the importance of ongoing investigations into H3K27me to refine and optimize management strategies for CNS tumors, paving the way for improved personalized medicine practices in oncology.
To Drink or Not to Drink? Investigating Alcohol’s Impact on Prostate Cancer Risk
Authors: Kaltsas Aris , Chrisofos Michael , Symeonidis Evangelos N. , Zachariou Athanasios , Stavropoulos Marios , Kratiras Zisis , Giannakodimos Ilias , Symeonidis Asterios , Dimitriadis Fotios , Sofikitis Nikolaos
Published: October 2024

Background/Objectives: Prostate cancer (PCa) is a significant global health issue. The relationship between alcohol consumption and PCa risk has been the subject of extensive research, yet findings remain inconsistent. This review aims to clarify the association between alcohol intake and PCa risk, its aggressiveness, and the potential metabolic pathways involved in PCa onset. Methods: A comprehensive literature search was conducted across multiple databases, including PubMed and MEDLINE, focusing on epidemiological studies, meta-analyses, cohort studies, and case–control studies. Studies evaluating alcohol consumption, prostate-specific antigen (PSA) levels, and PCa risk were included. The review also explored the roles of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in alcohol metabolism. Results: The analysis reveals a complex relationship between alcohol consumption and PCa. Heavy alcohol intake is associated with an increased risk of PCa, particularly more aggressive forms, and higher mortality rates. However, studies also show weak or no association between moderate alcohol consumption and PCa. The variability in findings may be attributed to differences in alcohol types, regional factors, and study methodologies. Conclusions: The link between alcohol consumption and PCa risk is multifaceted. While heavy drinking appears to increase the risk of aggressive PCa, the overall relationship remains unclear. Further research is needed to better understand these associations and inform public health recommendations and cancer prevention strategies.
Endoscopic Management of Post-Esophagectomy Delayed Gastric Conduit Emptying (DGCE): Results from a Cohort Study in a Tertiary Referral Center with Comparison between Procedures
Authors: Dell’Anna Giuseppe , Mandarino Francesco Vito , Fanizza Jacopo , Fasulo Ernesto , Barchi Alberto , Barà Rukaia , Vespa Edoardo , Viale Edi , Azzolini Francesco , Fanti Lorella , Battaglia Silvia , Puccetti Francesco , Cossu Andrea , Elmore Ugo , Fuccio Lorenzo , Annese Vito , Malesci Alberto , Rosati Riccardo , Danese Silvio
Published: October 2024

Background/Objectives: Delayed gastric conduit emptying (DGCE) occurs in 15–39% of patients who undergo esophagectomy. Intra-Pyloric Injection of Botulinum Toxin (IPBT), Pneumatic Balloon Dilation (PBD), and the same session combination (BTPD) represent the main endoscopic procedures, but comparative data are currently unavailable. Methods: We retrospectively analyzed prospectively collected data on all consecutive patients with DGCE treated endoscopically with IPBT, PBD, or BTPD. ISDE Diagnostic Criteria were used for DGCE diagnosis and classification. A Gastric Outlet Obstruction Score was used for clinical staging. All patients undergoing IPBT received 100 UI of toxin, while those undergoing PBD were dilated up to 20 mm. Clinical success (CS) was defined as the resolution of symptoms/resumption of feeding at discharge or expanding dietary intake at any rate. Recurrence was defined as symptom relapse after more than 15 days of well-being requiring endoscopic/surgical intervention. Results: A total of 64 patients (81.2% male, 90.6% Ivor-Lewis esophagectomy, 77.4% adenocarcinoma) with a median age of 62 years (IQR 55–70) were enrolled: 18 (28.1%) in the IPBT group, 24 (37.5%) in the PBD group, and 22 (34.4%) in the BTPD group. No statistically significant differences were found in the baseline characteristics, surgical techniques, and median follow-up among the three groups. BTPD showed a higher CS rate (100%) compared to the PD and BTPD groups (p = 0.02), and a Kaplan–Meier analysis with a log–rank test revealed that the BTPD group was associated both with a significatively shorter mean time to refeed of 1.16 days (95% CI 0.8–1.5; p = 0.001) and a shorter median time to discharge of one day (95% CI 1–3; p = 0.0001). Conclusions: Endoscopic management of DGCE remains challenging. Waiting for further strong evidence, BTPD can offer patients a higher clinical efficacy rate and a shorter time to refeed and be discharged.
CCL21 Induces Plasmacytoid Dendritic Cell Migration and Activation in a Mouse Model of Glioblastoma
Authors: Zhao Lei , Shireman Jack , Probelsky Samantha , Rigg Bailey , Wang Xiaohu , Huff Wei X. , Kwon Jae H. , Dey Mahua
Published: October 2024

Dendritic cells (DCs) are professional antigen-presenting cells that are traditionally divided into two distinct subsets: myeloid DCs (mDCs) and plasmacytoid DCs (pDCs). pDCs are known for their ability to secrete large amounts of cytokine type I interferons (IFN- α). In our previous work, we have demonstrated that pDC infiltration promotes glioblastoma (GBM) tumor immunosuppression through decreased IFN-α secretion via TLR-9 signaling and increased suppressive function of regulatory T cells (Tregs) via increased IL-10 secretion, resulting in poor overall outcomes in mouse models of GBM. Further dissecting the overall mechanism of pDC-mediated GBM immunosuppression, in this study, we identified CCL21 as highly upregulated by multiple GBM cell lines, which recruit pDCs to tumor sites via CCL21-CCR7 signaling. Furthermore, pDCs are activated by CCL21 in the GBM microenvironment through intracellular signaling of β-arrestin and CIITA. Finally, we found that CCL21-treated pDCs directly suppress CD8+ T cell proliferation without affecting regulatory T cells (Tregs) differentiation, which is considered the canonical pathway of immunotolerant regulation. Taken together, our results show that pDCs play a multifaced role in GBM immunosuppression, and CCL21 could be a novel therapeutic target in GBM to overcome pDC-mediated immunosuppression.
Holomics and Artificial Intelligence-Driven Precision Oncology for Medullary Thyroid Carcinoma: Addressing Challenges of a Rare and Aggressive Disease
Authors: Luvhengo Thifhelimbilu Emmanuel , Moeng Maeyane Stephens , Sishuba Nosisa Thabile , Makgoka Malose , Jonas Lusanda , Mamathuntsha Tshilidzi Godfrey , Mbambo Thandanani , Kagodora Shingirai Brenda , Dlamini Zodwa
Published: October 2024

Background/Objective: Medullary thyroid carcinoma (MTC) is a rare yet aggressive form of thyroid cancer comprising a disproportionate share of thyroid cancer-related mortalities, despite its low prevalence. MTC differs from other differentiated thyroid malignancies due to its heterogeneous nature, presenting complexities in both hereditary and sporadic cases. Traditional management guidelines, which are designed primarily for papillary thyroid carcinoma (PTC), fall short in providing the individualized care required for patients with MTC. In recent years, the sheer volume of data generated from clinical evaluations, radiological imaging, pathological assessments, genetic mutations, and immunological profiles has made it humanly impossible for clinicians to simultaneously analyze and integrate these diverse data streams effectively. This data deluge necessitates the adoption of advanced technologies to assist in decision-making processes. Holomics, which is an integrated approach that combines various omics technologies, along with artificial intelligence (AI), emerges as a powerful solution to address these challenges. Methods: This article reviews how AI-driven precision oncology can enhance the diagnostic workup, staging, risk stratification, management, and follow-up care of patients with MTC by processing vast amounts of complex data quickly and accurately. Articles published in English language and indexed in Pubmed were searched. Results: AI algorithms can identify patterns and correlations that may not be apparent to human clinicians, thereby improving the precision of personalized treatment plans. Moreover, the implementation of AI in the management of MTC enables the collation and synthesis of clinical experiences from across the globe, facilitating a more comprehensive understanding of the disease and its treatment outcomes. Conclusions: The integration of holomics and AI in the management of patients with MTC represents a significant advancement in precision oncology. This innovative approach not only addresses the complexities of a rare and aggressive disease but also paves the way for global collaboration and equitable healthcare solutions, ultimately transforming the landscape of treatment and care of patients with MTC. By leveraging AI and holomics, we can strive toward making personalized healthcare accessible to every individual, regardless of their economic status, thereby improving overall survival rates and quality of life for MTC patients worldwide. This global approach aligns with the United Nations Sustainable Development Goal 3, which aims to ensure healthy lives and promote well-being at all ages.
Exploring Factors Influencing Cervical Cancer Screening Participation among Singaporean Women: A Social Ecological Approach
Authors: Huang Qing , Tan Li-Ying
Published: October 2024

Background/Objectives: Cervical cancer screening uptake in Singapore remains suboptimal. This study employed the Social Ecological Model (SEM) to investigate factors influencing cervical cancer screening participation among Singaporean women. Methods: The study included 665 women, aged 25–69 years, who reported awareness of cancer screening and no personal cancer history. Data were collected through a previously described online survey. Hierarchical logistic regression analysis was conducted to identify significant factors influencing screening participation. Results: Only 30% of participants reported cervical cancer screening participation. Women aged 25–29 years (OR = 0.33; 95% CI = 0.12–0.77), Malay women (OR = 0.42; 95% CI = 0.20–0.83), and unmarried women (OR = 0.30; 95% CI = 0.18–0.48) were less likely to be screened. Positive associations with screening participation were observed with good cervical cancer screening knowledge (OR = 2.90; 95% CI = 1.96–4.32), awareness of primary care providers’ role in delivering screening services (OR = 1.94; 95% CI = 1.24–3.10), cancer information seeking behavior (OR = 1.59; 95% CI = 1.07–2.39), and acceptance of self-sampling options (OR = 1.81; 95% CI = 1.22–2.70). Conclusions: Our study highlights the cumulative impact of factors at various SEM levels on screening participation and underscores the necessity for more targeted and multi-pronged strategies to improve cervical cancer screening uptake in Singapore.
Optimizing Siglec-8-Directed Immunotherapy for Eosinophilic and Mast Cell Disorders
Authors: Lim Sheryl Y. T. , Huo Jenny , Laszlo George S. , Cole Frances M. , Kehret Allie R. , Li Junyang , Lunn-Halbert Margaret C. , Persicke Jasmyn L. , Rupert Peter B. , Strong Roland K. , Walter Roland B.
Published: October 2024

Background/Objective: Current treatments for eosinophilic and mast cell disorders are often ineffective. One promising target to improve outcomes is sialic acid-binding immunoglobulin-like lectin-8 (Siglec-8). As limitations, there are few Siglec-8 monoclonal antibodies (mAbs) available to date, and Siglec-8-directed treatments have so far primarily focused on unconjugated mAbs, which may be inadequate, especially against mast cells. Methods: Here, we used transgenic mice to raise a diverse panel of fully human mAbs that either recognize the V-set domain, membrane-distal C2-set domain, or membrane-proximal C2-set domain of full-length Siglec-8 as a basis for novel therapeutics. Results: All mAbs were efficiently internalized into Siglec-8-expressing cells, suggesting their potential to deliver cytotoxic payloads. Tool T cell-engaging bispecific antibodies (BiAbs) and chimeric antigen receptor (CAR)-modified natural killer (NK) cells using single-chain variable fragments from Siglec-8 mAbs showed highly potent cytolytic activity against Siglec-8-positive cells even in cases of very low target antigen abundance, whereas they elicited no cytolytic activity against Siglec-8-negative target cells. Siglec-8V-set-directed T cell-engaging BiAbs and Siglec-8V-set-directed CAR-modified NK cells induced substantially greater cytotoxicity against cells expressing an artificial smaller Siglec-8 variant containing only the V-set domain than cells expressing full-length Siglec-8, consistent with the notion that targeting membrane-proximal epitopes enhances effector functions of Siglec-8 antibody-based therapeutics. Indeed, unconjugated Siglec-8C2-set mAbs, Siglec-8C2-set-directed T cell-engaging BiAbs, and Siglec-8C2-set-directed CAR-modified NK cells showed high antigen-specific cytolytic activity against Siglec-8-positive human cell lines and primary patient eosinophils. Conclusions: Together, these data demonstrate Siglec-8-directed immunotherapies can be highly potent, supporting their further development for eosinophilic and mast cell disorders.
CREB Is Critically Implicated in Skin Mast Cell Degranulation Elicited via FcεRI and MRGPRX2
Authors: Li Zhuoran , Schneikert Jean , Tripathi Shiva Raj , Jin Manqiu , Bal Gürkan , Zuberbier Torsten , Babina Magda
Published: October 2024

Skin mast cells (MCs) mediate acute allergic reactions in the cutaneous environment and contribute to chronic dermatoses, including urticaria, and atopic or contact dermatitis. The cAMP response element binding protein (CREB), an evolutionarily well conserved transcription factor (TF) with over 4,000 binding sites in the genome, was recently found to form a feedforward loop with KIT, maintaining MC survival. The most selective MC function is degranulation with its acute release of prestored mediators. Herein, we asked whether CREB contributes to the expression and function of the degranulation-competent receptors FcεRI and MRGPRX2. Interference with CREB by pharmacological inhibition (CREBi, 666-15) or RNA interference only slightly affected the expression of these receptors, while KIT was strongly attenuated. Interestingly, MRGPRX2 surface expression moderately increased following CREB-knockdown, whereas MRGPRX2-dependent exocytosis simultaneously decreased. FcεRI expression and function were regulated consistently, although the effect was stronger at the functional level. Preformed MC mediators (tryptase, histamine, β-hexosaminidase) remained comparable following CREB attenuation, suggesting that granule synthesis did not rely on CREB function. Collectively, in contrast to KIT, FcεRI and MRGPRX2 moderately depend on unperturbed CREB function. Nevertheless, CREB is required to maintain MC releasability irrespective of stimulus, insinuating that CREB may operate by safeguarding the degranulation machinery. To our knowledge, CREB is the first factor identified to regulate MRGPRX2 expression and function in opposite direction. Overall, the ancient TF is an indispensable component of skin MCs, orchestrating not only survival and proliferation but also their secretory competence.
Generation of a Biotin-Tagged Dual-Display Phage
Authors: De Plano Laura Maria , Oddo Salvatore , Bikard David , Caccamo Antonella , Conoci Sabrina
Published: October 2024

Phage display is widely used in biomedical research. One of the great advantages of phage display is the specificity of the connection of a foreign peptide exposed outside the capsid to the intended target. Secondary detection systems, which are often laborious and costly, are required to identify and quantify the peptide/target interaction. In this study, we generated a novel dual-display phage to facilitate the detection and quantification of the peptide/target interaction. First, we generated a biotin-tagged phage by adding a small biotin-accepting peptide (sBT) to gene-3 of the M13K07 helper phage. Subsequently, we enhanced the M13K07 biotin-tagged phage by incorporating a selective peptide on gene-8, which is then exposed to the phage capsid. The exposed peptide acts as a probe to bind to a selective molecular target, whose interaction can be readily visualized thanks to the biotinylated phage. Our versatile dual-display phage exhibits high flexibility; by swapping the displayed peptide/probe, one can change the phage target while retaining the sBT gene in-frame with the pIII. We expect the generated biotin-tagged dual phages to be used as a multifunctional probe to couple with several streptavidin-biotin-based systems.
Modulatory Effects of Regulated Cell Death: An Innovative Preventive Approach for the Control of Mastitis
Authors: Xia Xiaojing , Ren Pengfei , Bai Yilin , Li Jingjing , Zhang Huihui , Wang Lei , Hu Jianhe , Li Xinwei , Ding Ke
Published: October 2024

Mastitis is a common disease worldwide that affects the development of the dairy industry due to its high incidence and complex etiology. Precise regulation of cell death and survival plays a critical role in maintaining internal homeostasis, organ development, and immune function in organisms, and regulatory abnormalities are a common mechanism of various pathological changes. Recent research has shown that regulated cell death (RCD) plays a crucial role in mastitis. The development of drugs to treat cell death and survival abnormalities that can be widely used in mastitis treatment has important clinical significance. This paper will review the molecular mechanisms of apoptosis, autophagy, pyroptosis, ferroptosis, and necroptosis and their regulatory roles in mastitis to provide a new perspective for the targeted treatment of mastitis.
Polo-like Kinase 1 Predicts Lymph Node Metastasis in Middle Eastern Colorectal Cancer Patients; Its Inhibition Reverses 5-Fu Resistance in Colorectal Cancer Cells
Authors: Poyil Pratheesh Kumar , Siraj Abdul K. , Padmaja Divya , Parvathareddy Sandeep Kumar , Alobaisi Khadija , Thangavel Saravanan , Begum Rafia , Diaz Roxanne , Al-Dayel Fouad , Al-Kuraya Khawla S.
Published: October 2024

Polo-like kinase 1 (PLK1) is a serine/threonine–protein kinase essential for regulating multiple stages of cell cycle progression in mammals. Aberrant regulation of PLK1 has been observed in numerous human cancers and is linked to poor prognoses. However, its role in the pathogenesis of colorectal cancer (CRC) in the Middle East remains unexplored. PLK1 overexpression was noted in 60.3% (693/1149) of CRC cases and was significantly associated with aggressive clinico-pathological parameters and p-ERK1/2 overexpression. Intriguingly, multivariate logistic regression analysis identified PLK1 as an independent predictor of lymph node metastasis. Our in vitro experiments demonstrated that CRC cells with high PLK1 levels were resistant to 5-Fu treatment, while those with low PLK1 expression were sensitive. To investigate PLK1′s role in chemoresistance, we used the specific inhibitor volasertib, which effectively reversed 5-Fu resistance. Interestingly, forced PLK1 expression activated the CRAF-MEK-ERK signaling cascade, while its inhibition suppressed this cascade. PLK1 knockdown reduced epithelial-to-mesenchymal transition (EMT) progression and stem cell-like traits in 5-Fu-resistant cells, implicating PLK1 in EMT induction and stemness in CRC. Moreover, silencing ERK1/2 significantly mitigated chemoresistance, EMT, and stemness properties in CRC cell lines that express PLK1. Furthermore, the knockdown of Zeb1 attenuated EMT and stemness, suggesting a possible link between EMT activation and the maintenance of stemness in CRC. Our findings underscore the pivotal role of PLK1 in mediating chemoresistance and suggest that PLK1 inhibition may represent a potential therapeutic strategy for the management of aggressive colorectal cancer subtypes.
Arrhythmias May Hide a Genetic Cardiomyopathy in Left Ventricular Hypertrabeculation in Children: A Single-Center Experience
Authors: Battipaglia Irma , Cantarutti Nicoletta , Cicenia Marianna , Adorisio Rachele , Battista Virginia , Baban Anwar , Silvetti Massimo Stefano , Drago Fabrizio
Published: October 2024

Background. Left ventricular hypertrabeculation (LVHT) is a myocardial disorder with different clinical manifestations, from total absence of symptoms to heart failure, arrhythmias, sudden cardiac death (SCD), and thromboembolic events. It is challenging to distinguish between the benign and pathological forms of LVHT. The aim of this study was to describe the arrhythmic manifestations of LVHT in a large group of pediatric patients and to correlate them with genetic results or other clinical markers. Methods. We retrospectively enrolled 140 pediatric patients with diagnosis of LVHT followed at our Institution from 2013 to 2023. Data regarding family history, instrumental exams, cardiac magnetic resonance, genetic testing and outcomes were collected. Most of them had isolated LVHT (80.7%); in other patients, mixed phenotypes (hypertrophic or dilated cardiomyopathy or congenital heart disease) were present. Results. Arrhythmias were found in 33 children (23.6%): 13 (9.3%) supraventricular tachyarrhythmias; 14 (10%) ventricular arrhythmias (five frequent PVCs (premature ventricular contractions), eight patients with ventricular tachycardia (VT), one ventricular fibrillation (VF)); two (1.4%) sinus node disfunctions; two (1.4%) complete atrio-ventricular blocks (AVB), three (2.1%) paroxysmal complete AVB, one (0.7%) severe I degree AVB. Three patients received an ICD (implantable cardioverter defibrillator). Comparison between LVHT patients with (33 pts) and without (107 pts) arrhythmias as regards genetic testing showed a statistical significance for the presence of class 4 or 5 genetic variants and arrhythmic manifestation (p = 0.037). Conclusions. In our pediatric cohort with LVHT, good outcomes were observed, but arrhythmias were not so rare (23.6%); no SCD occurred.
Does Climate Change Worry Decrease during Armed Conflicts?
Authors: Hamama-Raz Yaira , Shinan-Altman Shiri
Published: October 2024

Climate change stands out as an especially pressing global concern. The aim of the present study was to explore whether climate change worry decreases during armed conflicts, using two time-points: before and during an armed conflict. Guided by the Transactional Theory of Stress and Coping (TTSC), we examined the interplay between risk appraisal, pro-environmental behaviors (PEBs), and climate change worry. A sample of 202 Israeli adults participated in two waves of data collection, completing self-report measures addressing climate change worry, risk appraisal, and PEBs. Results revealed a significant decline in climate change worry and risk appraisal during the armed conflict, whereas PEBs remained unchanged. Contrary to expectations, the associations between risk appraisal, PEBs, and climate change worry did not weaken during the conflict. Mediation analyses indicated that the decline in risk appraisal led to a decline in PEBs, which subsequently contributed to a decline in climate change worry. However, this mediation effect was partial, with most of the association remaining direct. These findings imply that the psychological impact of armed conflict may temporarily overshadow environmental concerns, emphasizing the need for strategies to maintain environmental awareness and behavior even during an armed conflict.
On the Role of Substrate in Hydroxyapatite Coating Formation by Cold Spray
Authors: Henao John , Giraldo-Betancur Astrid , Poblano-Salas Carlos A. , Espinosa-Arbelaez Diego German , Corona-Castuera Jorge , Forero-Sossa Paola Andrea , Diaz-Rebollar Rene
Published: October 2024

The deposition of agglomerated hydroxyapatite (HAp) powders by low-pressure cold spray has been a topic of interest in recent years. Key parameters influencing the deposition of HAp powders include particle morphology and impact kinetic energy. This work examines the deposition of HAp powders on various metal surfaces to assess the impact of substrate properties on the formation of HAp deposits via cold spray. The substrates studied here encompass metals with varying hardness and thermal conductivities, including Al6061, Inconel alloy 625, AISI 316 stainless steel, H13 tool steel, Ti6Al4V, and AZ31 alloy. Single-track experiments offer insights into the initial interactions between HAp particles and different substrate surfaces. In this study, the results indicate that the ductility of the substrate may enhance HAp particle deposition only at the first deposition stages where substrate/particle interaction is the most critical factor for deposition. Features on the substrate associated with the first deposition sprayed layer include localized substrate deformation and the formation of clusters of HAp agglomerates, which aid in HAp deposition. Furthermore, after multiple spraying passes on the various metallic surfaces, deposition efficiency was significantly reduced when the build-up process of HAp coatings shifted from ceramic/metal to ceramic/ceramic interactions. Overall, this study achieved agglomerated HAp deposits with high deposition efficiencies (30–60%) through single-track experiments and resulted in the preparation of HAp coatings on various substrates with thickness values ranging from 24 to 53 µm. These coatings exhibited bioactive behavior in simulated body fluid.
Features of the Application of Coatings Based on the ZrN System to Increase Resistance to Mechanical Wear and Corrosion of Titanium Alloy Products
Authors: Volosova Marina , Zhylinski Valery , Sotova Catherine , Milovich Filipp , Seleznev Anton , Pyanka Hanna , Makarevich Kirill , Vereschaka Alexey
Published: October 2024

The coatings of ZrN, (Zr,Ti)N, (Ti,Zr,Hf)N and (Ti,Zr,Nb)N deposited on the titanium alloy substrate were compared. The wear resistance in the pin-on-disk test together with the Al2O3 indenter and the corrosion resistance in 3.5% NaCl solution were studied. It was found that the (Zr,Nb,Ti)N coating has the best resistance to wear, but has low corrosion resistance. The (Ti,Zr,Hf)N coating, on the contrary, has the best corrosion resistance, but low resistance to wear. The ZrN coating has good corrosion resistance combined with good resistance to wear. This coating is best suited for use in friction conditions with a ceramic counterbody under the influence of seawater. An important resource for increasing the properties of coatings is increasing their adhesion to the substrate, which can be achieved in two combined ways: (1) complete removal of the original oxide layer from the surface of the substrate and (2) the use of optimal compositions of the adhesive sublayer, which have not only high adhesive properties in relation to both the substrate and the coating, but also high strength. While the introduction of Nb into the ZrN coating composition increases wear resistance and the introduction of Hf increases corrosion resistance, the ZrN coating without additives best resists wear and corrosion simultaneously.
Preparation and Characterization of PLA-Based Films Fabricated with Different Citrus Species Peel Powder
Authors: Koutoulis Antonios S. , Giannakas Andreas E. , Lazaridis Dimitrios G. , Kitsios Apostolos-Panagiotis , Karabagias Vassilios K. , Giannakas Aris E. , Ladavos Athanasios , Karabagias Ioannis K.
Published: October 2024

The present study aimed to investigate whether different citrus peel powders (orange, mandarin, and lemon) could be incorporated into polylactic acid (PLA), for the preparation and characterization of biodegradable films made of this fruit waste. In particular, films were prepared by the solvent casting method and tested in terms of physicochemical and biochemical parameters, along with mechanical properties. Furthermore, the citrus peel powders were tested for their physicochemical and biochemical properties after ethanolic extraction. The results showed that the addition of citrus powders into PLA proved to be efficient compared to the control films (PLA). The citrus peel-based films showed high antioxidant capacity (38.37%–32.54%), a considerable total phenolic content (17.2–12.58 mg GAE/L), good lightness (L*: 42.83–41.93), elastic Young’s modulus (257.95–175.38 MPa), oxygen transmission rate (976.5 mL m−2 day−1–1218 mL m−2 day−1), elongation at break (183.06%–135.95%), and tensile strength (6.15–4.56 MPa), which were affected significantly (p < 0.05) by the botanical origin of citrus peel. Concerning the citrus peel extracts, the highest values in antioxidant capacity (52.56%), total phenolic content (724.8 mg GAE/L), and titratable acidity (714.67 mg of citric acid/L) were recorded in the extract of lemon peel powders. The corresponding values for orange peel powder extracts were 39.43%, 563.8 mg GAE/L, and 576 mg of citric acid/L, while those for mandarin were 33.01%, 558.46 mg GAE/L, and 54.67 mg of citric acid/L. The application of multivariate analysis of variance and linear discriminant analysis on the mechanical properties, physicochemical, and biochemical data resulted in the differentiation of pure PLA and PLA-based films fabricated with citrus peel powder. From the above findings, it is concluded that citrus peel powders are sources of phytochemicals and can be used for the preparation of PLA-based films for potential applications in food preservation.
Refining the Eel and Grouper Optimizer with Intelligent Modifications for Global Optimization
Authors: Kyrou Glykeria , Charilogis Vasileios , Tsoulos Ioannis G.
Published: October 2024

Global optimization is used in many practical and scientific problems. For this reason, various computational techniques have been developed. Particularly important are the evolutionary techniques, which simulate natural phenomena with the aim of detecting the global minimum in complex problems. A new evolutionary method is the Eel and Grouper Optimization (EGO) algorithm, inspired by the symbiotic relationship and foraging strategy of eels and groupers in marine ecosystems. In the present work, a series of improvements are proposed that aim both at the efficiency of the algorithm to discover the total minimum of multidimensional functions and at the reduction in the required execution time through the effective reduction in the number of functional evaluations. These modifications include the incorporation of a stochastic termination technique as well as an improvement sampling technique. The proposed modifications are tested on multidimensional functions available from the relevant literature and compared with other evolutionary methods.
Zero-Shot Learning for Accurate Project Duration Prediction in Crowdsourcing Software Development
Authors: Rashid Tahir , Illahi Inam , Umer Qasim , Jaffar Muhammad Arfan , Ramay Waheed Yousuf , Hakami Hanadi
Published: October 2024

Crowdsourcing Software Development (CSD) platforms, i.e., TopCoder, function as intermediaries connecting clients with developers. Despite employing systematic methodologies, these platforms frequently encounter high task abandonment rates, with approximately 19% of projects failing to meet satisfactory outcomes. Although existing research has focused on task scheduling, developer recommendations, and reward mechanisms, there has been insufficient attention to the support of platform moderators, or copilots, who are essential to project success. A critical responsibility of copilots is estimating project duration; however, manual predictions often lead to inconsistencies and delays. This paper introduces an innovative machine learning approach designed to automate the prediction of project duration on CSD platforms. Utilizing historical data from TopCoder, the proposed method extracts pertinent project attributes and preprocesses textual data through Natural Language Processing (NLP). Bidirectional Encoder Representations from Transformers (BERT) are employed to convert textual information into vectors, which are then analyzed using various machine learning algorithms. Zero-shot learning algorithms exhibit superior performance, with an average accuracy of 92.76%, precision of 92.76%, recall of 99.33%, and an f-measure of 95.93%. The implementation of the proposed automated duration prediction model is crucial for enhancing the success rate of crowdsourcing projects, optimizing resource allocation, managing budgets effectively, and improving stakeholder satisfaction.
Adaptive Energy Management Strategy for Hybrid Electric Vehicles in Dynamic Environments Based on Reinforcement Learning
Authors: Song Shixin , Zhang Cewei , Qi Chunyang , Song Chuanxue , Xiao Feng , Jin Liqiang , Teng Fei
Published: October 2024

Energy management strategies typically employ reinforcement learning algorithms in a static state. However, during vehicle operation, the environment is dynamic and laden with uncertainties and unforeseen disruptions. This study proposes an adaptive learning strategy in dynamic environments that adapts actions to changing circumstances, drawing on past experience to enhance future real-world learning. We developed a memory library for dynamic environments, employed Dirichlet clustering for driving conditions, and incorporated the expectation maximization algorithm for timely model updating to fully absorb prior knowledge. The agent swiftly adapts to the dynamic environment and converges quickly, improving hybrid electric vehicle fuel economy by 5–10% while maintaining the final state of charge (SOC). Our algorithm’s engine operating point fluctuates less, and the working state is compact compared with Deep Q-Network (DQN) and Deterministic Policy Gradient (DDPG) algorithms. This study provides a solution for vehicle agents in dynamic environmental conditions, enabling them to logically evaluate past experiences and carry out situationally appropriate actions.