bluuebunny/crossref_metadata_2025 huggingface dataset:
Absence of Heme Oxygenase-1 Affects Trophoblastic Spheroid Implantation and Provokes Dysregulation of Stress and Angiogenesis Gene Expression in the Uterus
Authors: Zenclussen Maria Laura , Ulrich Sina , Bauer Mario , Fink Beate , Zenclussen Ana Claudia , Schumacher Anne , Meyer Nicole
Published: February 2024

The enzyme heme oxygenase-1 (HO-1) is pivotal in reproductive processes, particularly in placental and vascular development. This study investigated the role of HO-1 and its byproduct, carbon monoxide (CO), in trophoblastic spheroid implantation. In order to deepen our understanding of the role of HO-1 during implantation, we conducted in vivo experiments on virgin and pregnant mice, aiming to unravel the cellular and molecular mechanisms. Using siRNA, HO-1 was knocked down in JEG-3 and BeWo cells and trophoblastic spheroids were generated with or without CO treatment. Adhesion assays were performed after transferring the spheroids to RL-95 endometrial epithelial cell layers. Additionally, angiogenesis, stress, and toxicity RT2-Profiler™ PCR SuperArray and PCR analyses were performed in uterine murine samples. HO-1 knockdown by siRNA impeded implantation in the 3D culture model, but this effect could be reversed by CO. Uteruses from virgin Hmox1−/− females exhibited altered expression of angiogenesis and stress markers. Furthermore, there was a distinct expression pattern of cytokines and chemokines in uteruses from gestation day 14 in Hmox1−/− females compared to Hmox1+/+ females. This study strongly supports the essential role of HO-1 during implantation. Moreover, CO appears to have the potential to compensate for the lack of HO-1 during the spheroid attachment process. The absence of HO-1 results in dysregulation of angiogenesis and stress-related genes in the uterus, possibly contributing to implantation failure.
Cultivating Pearls of Wisdom: Creating Protected Niche Spaces for Inner Transformations amidst the Metacrisis
Authors: Cooper Kira Jade , McIntyre Don G. , McCarthy Dan
Published: February 2024

The impetus for this paper emerges from the growing interest in leveraging inner transformations to support a global shift in ways of seeing and being. We caution that without sufficient individual and systemic maturity, inner transformations will be unable to hold the whole story and that attempts to drive paradigmatic shifts in ill-prepared systems will lead to insidious harms. As such, interventions for inner change will not have sufficient protected niche space to move beyond the boundaries of best practices towards wise practices. Drawing on Indigenous trans-systemics, we offer the metaphor of pearls as an invitation to recontextualize how inner transformations are conceived and approached in the metacrisis. To further develop this notion, we share a story of Wendigo and Moloch as a precautionary tale for the blind pursuit of inner and outer development. Weaving together metaphor, story, and scientific inquiry, we bring together Anishinaabe and Western knowledge systems for the purposes of healing and transformation. We hope that this paper will create space for wise practices—gifts from Creator to help sustain both Self and the World—to emerge, establish, and flourish. We invite readers on an exploration into the whole system of systems that are endemic to Anishinaabe cosmology, and a journey of reimagining new stories for collective flourishing amidst the metacrisis.
Reviving Antiquity in the Digital Era: Digitization, Semantic Curation, and VR Exhibition of Contemporary Dresses
Authors: Xhako Aldo , Katzourakis Antonis , Evdaimon Theodoros , Zidianakis Emmanouil , Partarakis Nikolaos , Zabulis Xenophon
Published: February 2024

In this paper, we present a comprehensive methodology to support the multifaceted process involved in the digitization, curation, and virtual exhibition of cultural heritage artifacts. The proposed methodology is applied in the context of a unique collection of contemporary dresses inspired by antiquity. Leveraging advanced 3D technologies, including lidar scanning and photogrammetry, we meticulously captured and transformed physical garments into highly detailed digital models. The postprocessing phase refined these models, ensuring an accurate representation of the intricate details and nuances inherent in each dress. Our collaborative efforts extended to the dissemination of this digital cultural heritage, as we partnered with the national aggregator in Greece, SearchCulture, to facilitate widespread access. The aggregation process streamlined the integration of our digitized content into a centralized repository, fostering cultural preservation and accessibility. Furthermore, we harnessed the power of these 3D models to transcend traditional exhibition boundaries, crafting a virtual experience that transcends geographical constraints. This virtual exhibition not only enables online exploration but also invites participants to immerse themselves in a captivating virtual reality environment. The synthesis of cutting-edge digitization techniques, cultural aggregation, and immersive exhibition design not only contributes to the preservation of contemporary cultural artifacts but also redefines the ways in which audiences engage with and experience cultural heritage in the digital age.
Review on Crystallization Strategies for Polymer Single Crystals
Authors: Wu Tianyu , Xu Jun , Ye Haimu
Published: February 2024

Polymer physics has evolved significantly over the past century, transitioning from the early recognition of the chain structure of polymers to a mature field integrating principles from statistical mechanics, thermodynamics, and condensed matter physics. As an important part of polymer physics, polymer single crystals are crucial for understanding molecular structures and behaviors, enhancing material properties, and enabling precise functionalization. They offer insights into polymer crystallization kinetics, serve as templates for nanofabrication, and have applications in electronics, sensors, and biomedical fields. However, due to the complexity of molecular chain movement, the formation of polymer single crystals is still very difficult. Over the decades, numerous researchers have dedicated themselves to unraveling the mysteries of polymer single crystals, yielding substantial findings. This paper focus on the historical evolution and advancements in polymer single crystal research, aiming to offer valuable insights and assistance to fellow researchers in this field.
Heterogeneous Nucleation Mechanism of Potassium Iodide on Graphene Surface in Water
Authors: Chen Yan-Nan , Liu Yu-Zhen , Sun Qiang
Published: February 2024

In this work, molecular dynamic (MD) simulations are applied to investigate the heterogeneous nucleation mechanism of KI on a graphene surface in water. As graphene is immersed in water, it mainly affects the structure of interfacial water (the topmost water layer at the interface between the substance and water). To maximize the hydrogen bonding of water, the dissolved solutes tend to accumulate to form the aggregate at the graphene surface, which undoubtedly affects the nucleation pathways of solutes in water. In comparison with homogeneous nucleation, a lower barrier may be expected during the heterogeneous nucleation of KI on a graphene surface in water. Therefore, as the graphene is immersed in water, this facilitates solute nucleation. From this work, it may be derived that heterogeneous nucleation may be closely related to the geometric characteristics of foreign surfaces, especially their geometric shape.
Polypoid Lesion Segmentation Using YOLO-V8 Network in Wireless Video Capsule Endoscopy Images
Authors: Sahafi Ali , Koulaouzidis Anastasios , Lalinia Mehrshad
Published: February 2024

Gastrointestinal (GI) tract disorders are a significant public health issue. They are becoming more common and can cause serious health problems and high healthcare costs. Small bowel tumours (SBTs) and colorectal cancer (CRC) are both becoming more prevalent, especially among younger adults. Early detection and removal of polyps (precursors of malignancy) is essential for prevention. Wireless Capsule Endoscopy (WCE) is a procedure that utilises swallowable camera devices that capture images of the GI tract. Because WCE generates a large number of images, automated polyp segmentation is crucial. This paper reviews computer-aided approaches to polyp detection using WCE imagery and evaluates them using a dataset of labelled anomalies and findings. The study focuses on YOLO-V8, an improved deep learning model, for polyp segmentation and finds that it performs better than existing methods, achieving high precision and recall. The present study underscores the potential of automated detection systems in improving GI polyp identification.
The Many Hidden Faces of Gallbladder Carcinoma on CT and MRI Imaging—From A to Z
Authors: Neculoiu Damaris , Neculoiu Lavinia Claudia , Popa Ramona Mihaela , Manea Rosana Mihaela
Published: February 2024

Gallbladder carcinoma represents the most aggressive biliary tract cancer and the sixth most common gastrointestinal malignancy. The diagnosis is a challenging clinical task due to its clinical presentation, which is often non-specific, mimicking a heterogeneous group of diseases, as well as benign processes such as complicated cholecystitis, xanthogranulomatous cholecystitis, adenomyomatosis, porcelain gallbladder or metastasis to the gallbladder (most frequently derived from melanoma, renal cell carcinoma). Risk factors include gallstones, carcinogen exposure, porcelain gallbladder, typhoid carrier state, gallbladder polyps and abnormal pancreaticobiliary ductal junction. Typical imaging features on CT or MRI reveal three major patterns: asymmetric focal or diffuse wall-thickening of the gallbladder, a solid mass that replaces the gallbladder and invades the adjacent organs or as an intraluminal enhancement mass arising predominantly from the gallbladder fundus. The tumor can spread to the liver, the adjacent internal organs and lymph nodes. Depending on the disease stage, surgical resection is the curative treatment option in early stages and adjuvant combination chemotherapy at advanced stages. The purpose of this scientific paper is to fully illustrate and evaluate, through multimodality imaging findings (CT and MRI), different presentations and imaging scenarios of gallbladder cancer in six patients and thoroughly analyze the risk factors, patterns of spread and differential diagnosis regarding each particular case.
Materials and Methods for All-Ceramic Dental Restorations Using Computer-Aided Design (CAD) and Computer-Aided Manufacturing (CAM) Technologies—A Brief Review
Authors: Solís Pinargote Nestor Washington , Yanushevich Oleg , Krikheli Natella , Smirnov Anton , Savilkin Sergey , Grigoriev Sergey N. , Peretyagin Pavel
Published: February 2024

The materials used in dentistry for the fabrication of all-ceramic restorations have undergone great and rapid developments over the last two decades. Among the most common ceramic materials in dentistry are those based on zirconium and lithium disilicate. Due to the properties of these materials, they are in great demand in the field of dental restoration production. Thus, dental restorations that will use those materials are commonly machined in CAD/CAM systems, which offer the possibility of manufacturing all-ceramic dental restorations in a very short period of time. This article reviews the modern materials in the field of all-ceramic dental restorations, their manufacturing processes, as well as what determines which ceramic materials are used for the production of CAD/CAM blanks and their production technology.
Green Finance and Fintech Adoption Services among Croatian Online Users: How Digital Transformation and Digital Awareness Increase Banking Sustainability
Authors: Serdarušić Hrvoje , Pancić Mladen , Zavišić Željka
Published: February 2024

This study delves into the dynamic interplay between green finance, Fintech adoption, digital awareness, and digital transformation in the Croatian banking industry. Amidst the emerging trend of sustainable banking practices and technological advancements, this research aims to examine the influence of green finance on Fintech adoption and banking sustainability. Employing a quantitative research design, this study gathered data through a survey questionnaire of 304 participants, comprising customers and employees of various banks in Croatia. The respondents’ insights were analyzed using IBM SPSS for the demographic analysis and SmartPLS for structural equation modeling (SEM). The results reveal a significant impact of green finance on Fintech adoption and digital awareness. Additionally, digital awareness significantly influenced Fintech adoption. However, the direct effect of digital transformation on Fintech adoption was not significant. This study also confirmed the significant influence of Fintech adoption on banking sustainability and identified the mediating role of digital awareness between green finance and Fintech adoption. This research contributes novel insights into the relationship between sustainable finance initiatives and digital banking trends. It underscores the need for increased digital awareness and the integration of green finance principles in the banking sector. These findings offer practical implications for banks in Croatia, suggesting a strategic focus on digital awareness programs, leveraging Fintech for enhanced customer experience, and fostering collaboration for a conducive Fintech environment.
Parental Perceived Usefulness on a School-Integrated App to Prevent Bullying and Eating Disorders
Authors: Cembreros Castaño Diana , Moraleda Ruano Álvaro , Nieto-Márquez Natalia Lara
Published: February 2024

The prevalence of bullying and eating disorders among adolescents is alarmingly high. In response to these issues, a mobile application called “Searching Help” was designed to be incorporated into school environments. This study aims to understand parents’ views on a school-integrated app designed to prevent bullying and eating disorders. Additionally, it sought to identify potential gender differences in these perceptions. Utilizing a retrospective research approach, we gathered insights from 201 parents of Spanish adolescents aged 12–18. The findings highlighted that a school-integrated app to prevent bullying and eating disorders was positively received by parents. Regarding gender, mothers were more aware of eating disorders and had greater concerns about unauthorized data access compared to fathers. Conversely, fathers were more optimistic about the potential of the app in creating a secure school environment against bullying and eating disorders. Notably, it was observed that although parents had a decent understanding of the problems associated with bullying and eating disorders independently, their knowledge of the technological solutions available to address these issues was considerably limited. In conclusion, this research confirms the gender-specific variations in parents’ attitudes and views toward technology-based solutions in schools and emphasizes a need for enhanced awareness initiatives to familiarize parents with relevant technological solutions for their children’s safety.
Patterns and Dominant Driving Factors of Carbon Storage Changes in the Qinghai–Tibet Plateau under Multiple Land Use Change Scenarios
Authors: Zhao Huihui , Yang Caifeng , Lu Miao , Wang Longhao , Guo Bing
Published: February 2024

Revealing the spatial–temporal evolution of carbon storage and its driving mechanisms in the Qinghai–Tibet Plateau could provide support for decision making in the protection of regional ecosystems and the achievement of regional dual-carbon goals. In this study, the spatial–temporal evolution of carbon storage in the Qinghai–Tibet Plateau was analyzed under various scenarios using PLUS-InVEST and a gravity center model, and the driving mechanisms of carbon storage were clarified with Geodetector. The results are as follows: (1) During 2000–2020, the areas of coniferous forest, evergreen broad-leaved forest, closed shrub, temperate shrub desert, multi-tree grassland, and grassland showed an increasing trend, while the areas of deciduous broad-leaved forest and mixed forest showed a decreasing trend. (2) During 2030–2060, there was a decreasing trend in the total carbon storage of the Qinghai–Tibet Plateau under three different scenarios. (3) During 2030–2060, the area of the Qinghai–Tibet Plateau was mostly represented by carbon balance (56%), while the areas of carbon sources and carbon sinks showed a scattered distribution. (4) The precipitation and topographic factors with a q value of 0.888 played a dominant role in affecting the spatio-temporal variations in carbon storage in the Qinghai–Tibet Plateau. (5) In future ecological protection and restoration efforts, more high-quality farmlands should be protected and constructed, which could contribute to the achievement of dual-carbon goals. In addition, the hydrothermal conditions should be improved to aid the carbon cycle process in the Qinghai–Tibet Plateau.
NH4+-N and Low Ratios of NH4+-N/NO3−-N Promote the Remediation Efficiency of Salix linearistipularis in Cd- and Pb-Contaminated Soil
Authors: Di Dongliu , Wang Shaokun , Chen Guangcai , Wang Qian , Zhang Jingwei , Niu Xiaoyun , Huang Dazhuang
Published: February 2024

(1) Background: the utilization of fast-growing trees for phytoremediation in heavy-metal-contaminated soil is increasingly recognized as an effective remediation method. Nitrogen (N) fertilizer enhances plant tolerance to heavy metals, yet the impact of various N levels and ammonium (NH4+-N)/nitrate (NO3−-N) ratios on the remediation of cadmium (Cd) and lead (Pb) by trees remains unclear. (2) Methods: the efficiency of Salix linearistipularis in remediating Cd- and Pb-contaminated soil was investigated using a pot experiment with three N levels (60, 120, 200 kg hm−1 year−1) and five NH4+-N/NO3−-N ratios (6/0, 4/2, 3/3, 2/4, 0/6) employed, resulting in 16 treatments including a control. (3) Results: the levels and ratios of NH4+-N/NO3−-N significantly affected the Cd and Pb uptake by S. linearistipularis. The highest increases in Cd and Pb in S. linearistipularis were observed for the N120-6/0 treatment, which increased by 104.36% and 95.23%, respectively. In addition, in the N120-6/0 treatment, the stem and leaf bioconcentration factors of Cd were significantly enhanced by 28.66% and 40.11%, respectively. Structural equation modeling revealed that the uptake of Cd and Pb was predominantly influenced by plant traits (biomass and root traits) rather than soil properties. (4) Conclusions: Our findings highlight the potential of the NH4+-N/NO3−-N ratio to regulate plant traits, thereby improving the phytoremediation efficiency of heavy-metal-contaminated soil.
Patterns in Tree Cavities (Hollows) in Euphrates Poplar (Populus euphratica, Salicaceae) along the Tarim River in NW China
Authors: Aishan Tayierjiang , Mumin Reyila , Halik Ümüt , Jiang Wen , Sun Yaxin , Yusup Asadilla , Chen Tongyu
Published: February 2024

Populus euphratica Oliv., an indicator species for eco-environmental change in arid areas, plays a key role in maintaining the stability of fragile oasis–desert ecosystems. Owing to human interference as well as to the harshness of the natural environment, P. euphratica forests have suffered severe damage and degradation, with trunk cavities (i.e., hollows) becoming increasingly pronounced, and thus posing a great threat to the growth, health, and survival of the species. Currently, there is a gap in our understanding of cavity formation and its distribution in P. euphratica. Here, cavities in the trunks and branches of a P. euphratica in a typical transect (Arghan) along the lower Tarim River were studied based on field positioning observations combined with laboratory analysis. The results revealed a large number of hollow-bearing P. euphratica stands in the study area; indeed, trees with hollows accounted for 56% of the sampled trees, with approximately 159 trees/ha. Sixty-six percent of hollow trees exhibited large (15 cm ≤ cavity width (CW) < 30 cm) or very large (CW > 30 cm) hollows. The main types of cavities in the trees were trunk main (31.3%), trunk top (20.7%), branch end (19.5%), and branch middle (19.5%). Tree parameters, such as diameter at breast height (DBH), tree height (TH), east–west crown width (EWCW), height under branches (UBH), and crown loss (CL) were significantly different between hollow and non-hollow trees. Both cavity height and width were significantly and positively correlated with DBH and CL, as well as with average crown width (ACW) (p < 0.001) and the distance from the tree to the river. The proportion of P. euphratica trees with cavities showed an overall increasing trend with increasing groundwater depth. Our findings show that cavities in P. euphratica varied with different tree architectural characteristics. Water availability is a major environmental factor influencing the occurrence of hollowing in desert riparian forests. The results provide scientific support for the conservation and sustainable management of existing desert riparian forest ecosystems.
Mechanism of Peppermint Extract-Induced Delay of ‘Packham’s Triumph’ Pear (Pyrus communis L.) Postharvest Ripening
Authors: Liang Chenglin , Jiang Fudong , Xu Hongpeng , Zhang Zan , Tian Wei , Sun Haifeng , Jing Yali , Wang Mengzhen , Zhuang Yingyu , Li Dingli , Liu Jianlong
Published: February 2024

Postharvest ripening is correlated to the quality and shelf life of European pear fruit. In this study, the effects of peppermint extract on fruit phenotype, related physiological activities, and aroma components during postharvest ripening of the European pear variety ‘Packham’s Triumph’ were examined. Fruit treated with 2.0 g L−1 peppermint extract for 12 h showed delayed softening by 4 d compared with that of the untreated control group. The peak values of ethylene and respiratory rate in fruit were reduced to a certain extent after peppermint extract treatment; however, the peppermint extract did not delay the occurrence of the respiratory climacteric peak. Peppermint extract treatment also did not significantly increase the content of the characteristic peppermint aroma in pear fruit. Further, widely targeted metabolome analysis revealed 298 significantly different metabolites, with flavonoids (40%) and lipid compounds (15%) accounting for the highest proportion on the first day after treatment. The Kyoto Encyclopedia of Genes and Genomes pathway result showed significant enrichment in the metabolic pathways of biosynthesis of flavonoid, isoflavonoid, flavone and flavonol, linoleic acid, and alpha-linolenic acid metabolism following peppermint extract treatment. The combined analysis of transcriptome and metabolome data showed significant enrichment in linoleic acid metabolism and alpha-linolenic acid metabolism on the first, third, and fifth days after peppermint extract treatment. This study indicates that peppermint extract mainly affects the pear fruit softening process in the early stage after treatment.
New Insight into the Effects of Endogenous Protein and Lipids on the Enzymatic Digestion of Starch in Sorghum Flour
Authors: Chao Chen , Liang Song , Zhang Zheyuan , Gidley Michael J. , Liu Ye , Wang Shujun
Published: February 2024

The effects of endogenous lipids and protein in sorghum flour on starch digestion were studied following the depletion of lipids and/or protein and after the reconstitution of separated fractions. The removal of protein or lipids moderately increases the digestibility of starch in raw (uncooked) sorghum flour to values close to those for purified starch. Rapid Visco Analyzer data (as a model for the cooking process) show that cooked sorghum flours with lipids have a lower starch digestibility than those without lipids after RVA processing, due to the formation of starch–lipid complexes as evidenced by their higher final viscosity and larger enthalpy changes. Additionally, the formation of a starch–lipid–protein ternary complex was identified in cooked sorghum flour, rather than in a reconstituted ternary mixture, according to the unique cooling stage viscosity peak and a greater enthalpy of lipid complexes. After heating, the sorghum flour showed a lower digestibility than the depleted flours and the reconstituted flours. The results indicate that the natural organization of components in sorghum flour is an important factor in facilitating the interactions between starch, lipids, and protein during RVA processing and, in turn, reducing the starch digestion.
High Pectin Recovery from Cocoa Husks Using an Autoclave Approach: An Analysis of Its Physicochemical, Structural, and Genotoxicity Properties
Authors: Pinkaew Thanaporn , Inthachat Woorawee , Khemthong Chanakan , Kemsawasd Varongsiri , On-Nom Nattira , Temviriyanukul Piya
Published: February 2024

Pectin was extracted from cocoa husks, a food-processing biowaste, using an autoclave approach. A Box–Behnken design (BBD) and response surface methodology (RSM) were used to optimize pectin extraction. Three factors including extraction time (5–40 min), temperature (105–135 °C), and solid to liquid ratios (SLRs) (10–30 w/v) were employed. Results showed that the optimal conditions for high cocoa-husk-pectin (CHP) yield of 26.22% was 105 °C for 5 min with an SLR at 20 w/v. The physicochemical characteristics of CHP were compared with commercial high-methoxyl pectin (CHMP) and commercial low-methoxyl pectin (CLMP). CHP was classified as low-methoxyl pectin, with a degree of esterification at 34.74% and methoxyl content of 5.08%. The galacturonic acid content of CHP was 32.71% which was lower than CHMP (72.69%) and CLMP (41.24%). The intrinsic viscosity and viscosity–average molecular weight was similar to CLMP but higher than CHMP. No significant differences in water-holding capacity were found among samples. CHP showed higher oil-holding capacity but lower solubility compared with commercial pectin. CHP solutions showed pseudoplastic behavior. The viscosities of CHP solutions improved at increasing concentrations and decreasing pH. The CHP solution viscosities were lower than CLMP at the same condition. The viscoelastic properties of CHP solutions increased at higher concentrations, with the optimal value at pH 3. CHP showed no genotoxicity when assayed using the Ames test. Autoclave extraction as an accessible fast method showed potential for high pectin yield recovery from cocoa husks.
Epigenetic Regulation in Schizophrenia: Focus on Methylation and Histone Modifications in Human Studies
Authors: Delphin Natasha , Aust Caitlin , Griffiths Lyn , Fernandez Francesca
Published: February 2024

Despite extensive research over the last few decades, the etiology of schizophrenia (SZ) remains unclear. SZ is a pathological disorder that is highly debilitating and deeply affects the lifestyle and minds of those affected. Several factors (one or in combination) have been reported as contributors to SZ pathogenesis, including neurodevelopmental, environmental, genetic and epigenetic factors. Deoxyribonucleic acid (DNA) methylation and post-translational modification (PTM) of histone proteins are potentially contributing epigenetic processes involved in transcriptional activity, chromatin folding, cell division and apoptotic processes, and DNA damage and repair. After establishing a summary of epigenetic processes in the context of schizophrenia, this review aims to highlight the current understanding of the role of DNA methylation and histone PTMs in this disorder and their potential roles in schizophrenia pathophysiology and pathogenesis.
Genotype-Specific Expression of Selected Candidate Genes Conferring Resistance to Leaf Rust of Rye (Secale cereale L.)
Authors: Azad Rumana , Krępski Tomasz , Olechowski Mateusz , Biernacik Bartosz , Święcicka Magdalena , Matuszkiewicz Mateusz , Dmochowska-Boguta Marta , Rakoczy-Trojanowska Monika
Published: February 2024

Leaf rust (LR) caused by Puccinia recondita f. sp. secalis (Prs) is a highly destructive disease in rye. However, the genetic mechanisms underlying the rye immune response to this disease remain relatively uncharacterised. In this study, we analysed the expression of four genes in 12 rye inbred lines inoculated with Prs at 20 and 36 h post-treatment (hpt): DXS (1-deoxy-D-xylulose 5-phosphate synthase), Glu (β-1,3-glucanase), GT (UDP-glycosyltransferase) and PR-1 (pathogenesis-related protein 1). The RT-qPCR analysis revealed the upregulated expression of the four genes in response to Prs in all inbred lines and at both time-points. The gene expression data were supported by microscopic and macroscopic examinations, which revealed that eight lines were susceptible to LR and four lines were highly resistant to LR. A relationship between the infection profiles and the expression of the analysed genes was observed: in the resistant lines, the expression level fold changes were usually higher at 20 hpt than at 36 hpt, while the opposite trend was observed in the susceptible lines. The study results indicate that DXS, Glu, GT and PR-1 may encode proteins crucial for the rye defence response to the LR pathogen.
The Impact of the COVID-19 Pandemic on Diabetes Self-Management in Saudi Arabia
Authors: Sales Ibrahim , Bawazeer Ghada , Shahba Ahmad Abdul-Wahhab , Alkofide Hadeel
Published: February 2024

The COVID-19 pandemic disrupted healthcare worldwide, potentially impacting disease management. The objective of this study was to assess the self-management behaviors of Saudi patients with diabetes during and after the COVID pandemic period using the Arabic version of the Diabetes Self-Management Questionnaire (DSMQ). A cross-sectional study was conducted in patients aged ≥18 years diagnosed with type 2 diabetes mellitus who had at least one ambulatory clinic visit in each of the specified time frames (Pre-COVID-19: 1 January 2019–21 March 2020; COVID-19 Time frame: 22 March 2020 to 30 April 2021) utilizing the DSMQ questionnaire, with an additional three questions specifically related to their diabetes care during the COVID pandemic. A total of 341 patients participated in the study. The study results revealed that the surveyed patients showed moderately high self-care activities post-COVID-19. Total DSMQ scores were significantly higher in patients aged >60 years versus younger groups (p < 0.05). Scores were significantly lower in patients diagnosed for 1–5 years versus longer durations (p < 0.05). Patients on insulin had higher glucose management sub-scores than oral medication users (p < 0.05). Overall, DSMQ scores were higher than the pre-pandemic Saudi population and Turkish post-pandemic findings. DSMQ results suggest that, while COVID-19 negatively impacted some self-management domains, the Saudi patients surveyed in this study upheld relatively good diabetes control during the pandemic. Further research is warranted on specific barriers to optimize diabetes care during public health crises.
An Urgent Call for Concussion Incidence Measures in Para Sport for Athletes with Vision Impairment: A Narrative Review
Authors: Teodoro Juliette I. , Irving Elizabeth L. , Blaine Jane D. , Dalton Kristine N.
Published: February 2024

Concussion in para athletes with vision impairment (VI) is poorly understood. Recently published studies have suggested that athletes with VI may be more likely to sustain sport-related concussions compared to non-disabled athletes and athletes with other impairment types. There is a critical need for objective concussion incidence measures to determine concussion injury rates and risks more accurately. The aim of this review was to examine the limited available evidence of concussion incidence rates across six different para sports for athletes with VI and encourage the future collection of concussion incidence data and the adoption of injury prevention strategies in VI para sport. A literature search was conducted using four unique databases, which formed the basis of this narrative review. Injury prevention strategies such as modifying sport rules, introducing protective equipment, and incorporating additional safety measures into the field of play have been introduced sporadically, but the effectiveness of most strategies remains unknown. More prospective, sport-specific research examining mechanisms of injury and risk factors for concussion injuries in athletes with VI in both training and competition is needed. This research will help inform the development of targeted injury prevention strategies to reduce the likelihood of concussion for athletes with VI.
Response of Chironomidae (Diptera) to DDT, Mercury, and Arsenic Legacy Pollution in Sediments of the Toce River (Northern Italy)
Authors: Marziali Laura , Pirola Niccolò , Schiavon Alfredo , Rossaro Bruno
Published: February 2024

The Toce River (Northern Italy) is characterized by legacy contamination of dichloro-diphenyl-trichloroethane (DDT), mercury, and arsenic deriving from an industrial plant active between ca. 1915 and 1996. Chironomidae taxa assemblages and sediments were collected in 2014 and 2019 upstream and downstream of the industrial area to analyze species responses to toxic substances in a river stretch with relatively uniform natural (i.e., hydro-morphological) characteristics. A total of 32 chironomid taxa were identified. Sediment concentrations reached levels potentially toxic for benthic invertebrates: 15.7 µg kg−1 1% organic carbon for DDT, 197 µg kg−1 dry weight (d.w.) for Hg, and 55.7 mg kg−1 d.w. for As. Canonical Correspondence Analysis (CCA) revealed a predominant seasonal gradient, followed by an upstream-downstream gradient. Partial CCA indicated that 5.2% of the total variation was associated with sediment contamination. Self-Organizing Maps (SOMs) were used to represent species responses to toxicants. Most species appeared to be tolerant, e.g., Chironomus riparius, Micropsectra atrofasciata, Conchapelopia pallidula, and Polypedilum spp. Sensitivity to contaminants was observed in only a few species: Diamesa spp., Sympotthastia spinifera, and Prodiamesa olivacea to DDT; Potthastia longimanus to Hg; Odontomesa fulva and Microtendipes pedellus to As. The chironomid community was characterized in presence of contamination levels commonly observed in freshwater ecosystems.
Human Neutrophil Alpha-Defensins Promote NETosis and Liver Injury in Alcohol-Related Liver Cirrhosis: Potential Therapeutic Agents
Authors: Rycyk-Bojarzyńska Anna , Kasztelan-Szczerbińska Beata , Cichoż-Lach Halina , Surdacka Agata , Roliński Jacek
Published: February 2024

Background: Neutrophils are thought to play a pivotal role in the pathogenesis of many inflammatory diseases, such as hepatitis, liver cirrhosis, etc. Activated human neutrophils release human neutrophil peptides (HNP1-3) or alpha-defensins that are antimicrobial peptides in azurophil granules. Furthermore, HNP1-3 build a scaffold of neutrophil extracellular traps (NETs) and promote the process of programmed cell death called NETosis. Our study aimed to investigate the role of alpha-defensins in the pathogenesis of alcohol-related liver cirrhosis (ALC). Methods: The concentrations of alpha-defensins in the plasma of 62 patients with ALC and 24 healthy subjects were measured by ELISA. The patients with ALC were prospectively recruited based on the severity of liver dysfunction according to the Child-Pugh and Model of End-Stage Liver Disease-Natrium (MELD-Na) scores, modified Maddrey’s Discriminant Function (mDF), and the presence of ALC complications. Results: The concentrations of alpha-defensins in plasma were significantly higher in the ALC patients than in the controls. The plasma levels of HNP1-3 correlated with the MELD and mDF scores. ALC subgroups with MELD > 20 and mDF > 32 displayed significantly higher HNP1-3 concentrations. The plasma levels of HNP1-3 revealed a good predictive AUC for hepatic encephalopathy and ascites development (0.81 and 0.74, respectively) and for patient survival (0.87) in those over 40 years of age. Conclusion: These findings suggest that alpha-defensins play an important role in the assessment of ALC.
The Use of Medicinal Leeching in Breast Surgery: A Systematic Review
Authors: Rajaram Rohan , Cevik Jevan , Bhindi Nayan , Seth Ishith , Rozen Warren M.
Published: February 2024

Background: The medicinal leech has been used in plastic surgery to resolve venous congestion that can threaten the viability of tissue transfer. Within the context of breast surgery, venous congestion is a pertinent consideration for reconstructive and non-reconstructive breast surgery such as mammoplasty and mastopexy. However, leeching is closely associated with complications such as infection, pain, and anaemia. This is the first systematic review that examines the methodology, efficacy, and post therapeutic outcome data across all existing studies on medicinal leeching in breast surgery. Methods: A systematic search of PubMed and Embase databases from their inception to November 2023 was conducted. Inclusion criteria included studies reporting on the use of leeches to resolve venous congestion in any breast surgery. The JBI Critical Appraisal Checklist for Case Series tool was used for bias analysis. Descriptive statistics were undertaken in Microsoft Excel. Results: A total of 18 studies with a combined sample size of 28 were examined, including 4 case series and 14 case reports. Patients mostly underwent reconstructive breast surgery (75%). The median number of leeches used was two, with a median number of three leeching sessions per day and 3 days of leeching. Medicinal leeching successfully prevented the loss of 75% of all tissue transfers. The complication rate was high at 81.14% and mainly included infection and anaemia. Conclusions: Medicinal leeching is an effective method to relieve venous congestion in breast surgery but must be judiciously used within the clinical context of the patient to maximise efficacy and mitigate harm from complications.
The Impact of Anti-Amyloid Immunotherapies on Stroke Care
Authors: Bilodeau Philippe A. , Dickson John R. , Kozberg Mariel G.
Published: February 2024

Anti-amyloid immunotherapies have recently emerged as treatments for Alzheimer’s disease. While these therapies have demonstrated efficacy in clearing amyloid-β and slowing cognitive decline, they have also been associated with amyloid-related imaging abnormalities (ARIA) which include both edema (ARIA-E) and hemorrhage (ARIA-H). Given that ARIA have been associated with significant morbidity in cases of antithrombotic or thrombolytic therapy, an understanding of mechanisms of and risk factors for ARIA is of critical importance for stroke care. We discuss the latest data regarding mechanisms of ARIA, including the role of underlying cerebral amyloid angiopathy, and implications for ischemic stroke prevention and management.
Energy-Aware Integrated Scheduling for Quay Crane and IGV in Automated Container Terminal
Authors: Luo Yuedi , Liang Xiaolei , Zhang Yu , Tang Kexin , Li Wenting
Published: February 2024

In this study, we address the integrated scheduling problem involving quay cranes and IGVs in automated terminals. We construct a mixed-integer planning model with the aim of minimizing the total energy consumption during quay crane and IGV operations, focusing on the loading-operation mode. The model considers the impact of the actual stowage of container ships on the loading order. We propose a dimension-by-dimension mutation sparrow search algorithm to optimize the model’s solution quality. Building upon the standard sparrow search algorithm, we incorporate cat mapping to enhance the diversity of the initial sparrow population. To improve global search in the early stage and local search in the later stage of the algorithm, we introduce an adaptive t-distribution mutation strategy. Finally, a total of 12 instances with container counts containing 30, 100, and 250 were designed for experiments to validate the effectiveness of the model and algorithm. The experiments demonstrate that, by appropriately increasing the number of quay cranes, configuring more than two or three IGVs can achieve optimal energy consumption for overall operations.
Revisiting the Institut Mutualiste Montsouris Difficulty Classification of Laparoscopic Liver Resection with the Data from a Personal Series—Evaluations for the Difficulty of Left Medial Sectionectomy and Length of Hospital Stay
Authors: Morise Zenichi
Published: February 2024

The IMM (Institut Mutualiste Montsouris) difficulty classification for laparoscopic liver resection is based only on the type of surgical procedure. It is useful for assessing outcomes and setting benchmarks in a group sharing the same indications. There is, however, no left medial sectionectomy in the system. Its difficulty was evaluated using the same methodology as IMM with the data from a personal series. Furthermore, length of hospital stay was evaluated as the representative of short-term outcomes. IMM scores of our right and left hemihepatectomies, right anterior sectionectomy, and segment 7 or 8 segmentectomies are 3. That of left medial sectionectomies is 2, the same as right posterior sectionectomy and segment or less anatomical resections. Those of left lateral sectionectomy and partial resection are 0. The group with a score of 3 was divided into two groups—with and without extended hospital stays (extended only for right hemihepatectomies and right anterior sectionectomies). The difficulty of medial sectionectomy was positioned at the same level as posterior sectionectomy and segment or less anatomical resections. The result from the second evaluation may indicate that there are other factors with an impact on difficulty related to short-term outcomes, other than intraoperative surgical difficulty from the procedure itself.
Draft Genome Sequence Analyses of Two Novel Marinobacter suadae sp. nov. and Wenyingzhuangia gilva sp. nov. Isolated from the Root of Suaeda japonica Makino
Authors: Park Sunho , Kim Inhyup , Chhetri Geeta , Jung Yonghee , Woo Haejin , Seo Taegun
Published: February 2024

Gram-negative, rod-shaped, and aerobic bacteria designated chi1T and chi5T were isolated from the root of Suaeda japonica Makino. Phylogenetics utilizing 16S rRNA and whole-genome sequences of the two novel strains chi1T and chi5T confirmed that they were related to the genera Marinobacter and Wenyingzhuangia, respectively. For the novel strains chi1T and chi5T, the digital DNA–DNA hybridization values (19–20% and 22.1–36.6%, respectively) and average nucleotide identity values (74.4–76.5% and 79.1–88.9%, respectively) fell within the range for the genera Marinobacter and Wenyingzhuangia, respectively. Pangenome analyses of the novel strains chi1T and chi5T revealed 357 and 368 singletons genes, respectively. The genomic DNA G + C contents of the strains chi1T and chi5T were 57.2% and 31.5%, respectively. The major fatty acids of strain chi1T were C12:0, C16:0, and summed feature 3 (C16:1 ω6c and/or C16:1ω7c), while those of the strain chi5T were iso-C15:0 3OH, iso-C17:0 3OH, and iso-C15:0. Data from the phylogenetic, phylogenomic, pangenome, genomic, physiological, and biochemical analyses indicated that the novel strains were distinct. Therefore, we propose the names Marinobacter suadae (type strain chi1T = KACC 23259T = TBRC 17652T) and Wenyingzhangia gilva (type strain chi5T = KACC 23262T = TBRC 17900T) for the studied bacterial strains.
Screening and Isolation of Xylanolytic Filamentous Fungi from the Gut of Scarabaeidae Dung Beetles and Dung Beetle Larvae
Authors: Makulana Livhuwani , La Grange Daniel C. , Moganedi Kgabo L. M. , Mert Marlin J. , Phasha Nkateko N. , Jansen van Rensburg Elbert L.
Published: February 2024

Research on renewable biotechnology for renewable biofuel applications has reached new heights. This is highlighted by extensive biomining for novel enzymes to reduce the production costs from animal and insect gut microbiomes. This study explored the diversity and composition of hemicellulolytic fungi in the gut microbiota from dung beetles of the family Scarabaeidae (Pachylomerus femoralis, Anachalcos convexus and Euoniticellus intermedius). Two hundred and twenty-two filamentous fungi were isolated, purified and identified using rDNA sequencing of the ITS and D1/D2 regions. The fungal isolates were assigned to 12 genera and 25 species. Fungi associated with the genus Aspergillus was in abundance, with Hypocrea lixii predominantly isolated. Isolates that produced more than 3 U/mL of xylanase activity were evaluated further. The highest xylanase activity was of 23.6 and 23.5 U/mL for L1XYL9 (E. intermedius larvae) and Hypocrea lixii AB2A3 (A. convexus), respectively. Phylogeny of the fungal strains with xylanolytic activity was analysed using ITS rDNA sequences and revealed close genetic relatedness between isolates from the different dung beetle species. Fungal genera commonly found in the gut of both adult beetles and larvae included Aspergillus, Hypocrea, Talaromyces and Penicillium. The results obtained in this study suggest that the gut of Scarabaeidae dung beetles in South Africa is a rich source of xylanolytic fungi.
In Situ Polymer-Solution-Processed Graphene–PDMS Nanocomposites for Application in Intracranial Pressure Sensors
Authors: Hong Hua , Zhang Junjie , Zhu Yuchen , Tse Stephen D. , Guo Hongxuan , Lai Yilin , Xi Yubo , He Longbing , Zhu Zhen , Yin Kuibo , Sun Litao
Published: February 2024

Polydimethylsiloxane (PDMS) has emerged as a promising candidate for the dielectric layer in implantable sensors due to its exceptional biocompatibility, stability, and flexibility. This study introduces an innovative approach to produce graphene-reinforced PDMS (Gr-PDMS), where graphite powders are exfoliated into mono- and few-layer graphene sheets within the polymer solution, concurrently forming cross-linkages with PDMS. This method yields a uniformly distributed graphene within the polymer matrix with improved interfaces between graphene and PDMS, significantly reducing the percolation threshold of graphene dispersed in PDMS from 10% to 5%. As-synthesized Gr-PDMS exhibits improved mechanical and electrical properties, tested for potential use in capacitive pressure sensors. The results demonstrate an impressive pressure sensitivity up to 0.0273 kpa−1, 45 times higher than that of pristine PDMS and 2.5 times higher than the reported literature value. The Gr-PDMS showcases excellent pressure sensing ability and stability, fulfilling the requirements for implantable intracranial pressure (ICP) sensors.
Direct Growth of Low Thermal Conductivity WTe2 Nanocrystalline Films on W Films
Authors: Yu Zhisong , Tao Rong , Guo Jin , Feng Shiyi , Wang Yue
Published: February 2024

WTe2 has attracted much attention because of its layered structure and special electronic energy band structure. However, due to the difficulty of evaporating the W element itself and the inactivity of the Te element, the obtained large-area WTe2 thin films are usually accompanied by many defects. In this paper, WTe2 nanocrystalline films were successfully prepared on quartz substrates using magnetron sputtering and chemical vapor deposition techniques. Various analytical techniques such as X-ray Diffraction, Raman spectra, X-ray Photoelectron Spectroscopy, Scanning Electron Microscope, and photoluminescence spectra are employed to analyze the crystal structure, composition, and morphology. The effects of different tellurization temperatures and tellurization times on the properties of WTe2 thin films were investigated. WTe2 nanocrystalline films with good crystallinity were obtained at 600 °C for 30 min. The thermal conductivity of the WTe2 films prepared under this condition was 1.173 Wm−1K−1 at 300 K, which is significantly higher than that of samples prepared using other methods.