bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Questionnaire Length and Behavioral Consequences on	Careless Responding
Authors: Gibson Anthony M. , Bowling Nathan A.
Published: July 2019

Abstract. The current paper reports the results of two randomized experiments designed to test the effects of questionnaire length on careless responding (CR). Both experiments also examined whether the presence of a behavioral consequence (i.e., a reward or a punishment) designed to encourage careful responding buffers the effects of questionnaire length on CR. Collectively, our two studies found (a) some support for the main effect of questionnaire length, (b) consistent support for the main effect of the consequence manipulations, and (c) very limited support for the buffering effect of the consequence manipulations. Because the advancement of many subfields of psychology rests on the availability of high-quality self-report data, further research should examine the causes and prevention of CR.
Measurement Invariance Across Age Groups and Over 20 Years’	Time of the Negative and Positive Affect Scale (NAPAS)
Authors: Chan Meingold Hiu-ming , Gerhardt Micah , Feng Xin
Published: July 2019

Abstract. The factor structure and measurement invariance across gender of Mroczek’s and Kolarz’s scales of positive affect (PA) and negative affect (NA) have been examined in past studies; however, little is known about the measurement invariance across age groups and over time, which are important psychometric properties for developmental research. The current study sought to fill this gap using the data from the National Survey of Midlife Development in the United States (MIDUS). Multi-group confirmatory factor analysis (CFA) was used to examine increasing levels of measurement invariance across gender and age groups. Longitudinal CFA was also used to test measurement invariance over three time points using the data from MIDUS 1 ( N = 3,748), MIDUS 2 ( N = 2,257), and MIDUS 3 ( N = 1,414). Results supported full scalar invariance across gender, age groups, and over time. The latent means for NA were significantly different between men and women at time 1 and 2, but not at time 3; the latent means for both PA and NA were also different across age groups. There were no significant differences for PA and only trivial differences for NA over time within individuals. Implications of these results for longitudinal research are discussed.
Assessing Other-Directed, Lighthearted, Intellectual, and Whimsical	Playfulness in Adults
Authors: Proyer René T. , Brauer Kay , Wolf Annegret
Published: July 2019

Abstract. Adult playfulness is an individual differences variable that allows people to (re-)frame everyday situations in a way to make them entertaining, stimulating, and/or interesting. Recently, a structural model with four facets (i.e., Other-directed, Lighthearted, Intellectual, and Whimsical; OLIW) and a subjective measure with 28 items have been developed. The main aim of this study is to test a 12-item short form (OLIW-S). In Study 1, we retrieved the 4-factorial OLIW structure in three independently collected samples ( N = 1,168; 696 self-ratings and 166 dyads providing reciprocal self- and peer-ratings). We found satisfactory psychometric properties for research purposes, and there was convergent and nomological validity with external measures of playfulness and the Big Five personality traits. Data from Sample 3 showed robust self-other agreement in all facets (.27–.52). In Study 2, N = 463 adults completed the OLIW-S and a measure for personality pathology (PID-5; shared variance 12–31%). A principal component analysis of the OLIW-S and PID-5 scales yielded two unique factors representing playfulness and personality pathology. Overall, our expectations were widely met and the OLIW-facets could be well located in comprehensive systems of both personality and personality pathology. Applications of the OLIW-S (e.g., large-scale assessments; zero-acquaintance studies) are discussed.
More Than Hitting the Correct Key Quickly
Authors: Gaschler Robert , Zhao Fang , Röttger Eva , Panzer Stefan , Haider Hilde
Published: July 2019

Abstract. Many studies have documented that multitasking reduces Response Time (RT) indicators of implicit sequence learning as well as the expression of acquired sequence knowledge in RT benefits. In these tasks it is only relevant that the correct key is hit quickly, not where it is hit. We explored how variability in response location is influenced by (a) breaking a repeating sequence of target locations, (b) multitasking demands in the current trial, and (c) presence of multitasking in the block. Participants performed a Serial Reaction Time Task (SRTT) on a touchscreen while shutting down a beep tone by pressing the space bar with their non-dominant hand (throughout Experiment 1 and in the second half of Experiment 2). The first-order sequence of four response locations on the screen was broken by off-sequence deviants in 1/6th of the trials. Our results show a dissociation between RT and response location variability. While the effect of breaking the sequence on RT was larger under single- than under multitasking, breaking the sequence only led to an increase in response location variability under multitasking. Experiment 3 suggested that the impact of sequence knowledge on either aspect of performance in the SRTT is limited by interference from an additional task.
Transcending Science: Can Artists Help Scientists Save the World?
Authors: Tosca Mika
Published: July 2019

The artistic process begins with human engagement. Perhaps the revolution we need to address climate change begins by making it an integral part of the scientific method.
High‐Frequency Wave Generation in Magnetotail Reconnection: Nonlinear Harmonics of Upper Hybrid Waves
Authors: Dokgo Kyunghwan , Hwang Kyoung‐Joo , Burch James L. , Choi Eunjin , Yoon Peter H. , Sibeck David G. , Graham Daniel B.
Published: July 2019

MMS3 spacecraft passed the vicinity of the electron diffusion region of magnetotail reconnection on 3 July 2017, observing discrepancies between perpendicular electron bulk velocities and
drift, and agyrotropic electron crescent distributions. Analyzing linear wave dispersions, Burch et al. (2019, https://doi.org/10.1029/2019GL082471) showed the electron crescent generates high‐frequency waves. We investigate harmonics of upper‐hybrid (UH) waves using both observation and particle‐in‐cell (PIC) simulation, and the generation of electromagnetic radiation from PIC simulation. Harmonics of UH are linearly polarized and propagate along the perpendicular direction to the ambient magnetic field. Compared with two‐dimensional PIC simulation and nonlinear kinetic theory, we show that the nonlinear beam‐plasma interaction between the agyrotropic electrons and the core electrons generates harmonics of UH. Moreover, PIC simulation shows that agyrotropic electron beam can lead to electromagnetic (EM) radiation at the plasma frequency and harmonics.
Investigating the Influence of Cloud Radiative Effects on the Extratropical Storm Tracks
Authors: Grise Kevin M. , Medeiros Brian , Benedict James J. , Olson Jerry G.
Published: July 2019

Recent studies have focused on the role of cloud radiative effects (CRE) in governing the mean atmospheric circulation and its response to climate change. This study instead examines the role of CRE in climate variability in the extratropics. Cloud locking experiments are performed using the Community Earth System Model. In these experiments, CRE are scrambled, such that they maintain the same climatology but no longer match the model's dynamical fields. The results of these experiments indicate that high‐frequency interactions between CRE and dynamics have a small (≤5–10%) but statistically significant damping effect on the intensity of the extratropical storm tracks, particularly in the Southern Hemisphere. Individual midlatitude cyclones have decreased intensity and shorter lifetime. These effects arise largely from clouds' radiative modification of static stability below 700 hPa. The coupling among clouds, radiation, and dynamics thus has a modest but potentially important influence on the extratropical storm tracks.
Application of Remote Sensing to Identify and Monitor Seasonal and Interannual Changes of Water Turbidity in Yellow River Estuary, China
Authors: Wang Siyuan , Shen Ming , Ma Yuanxu , Chen Guangsheng , You Yongfa , Liu Weihua
Published: July 2019

Water turbidity is an important indicator for water security and environmental security in the Yellow River estuary. However, due to the complex terrain and harsh climatic environment, it is difficult to monitor the water turbidity over the complex surface of the estuary. In this study we applied a self‐organizing map clustering method, an artificial neural network clustering method, to extract turbidity patterns from the long‐term remote sensing data sets in the Yellow River estuary. Based on the Moderate Resolution Imaging Spectroradiometer data from 2000 to 2015, six turbidity patterns were identified by using the self‐organizing map clustering method: high turbidity pattern, moderate turbidity pattern, low turbidity pattern, very low turbidity pattern, extreme high turbidity pattern and sea ice pattern, and the first four patterns appear every year. All patterns have significant seasonal characteristics, and monthly turbidity is dominated by one of these turbidity patterns. The water turbidity in the estuary has decreased in the past 16 years, and the interannual variation of the turbidity pattern is the result of the combination of the sediment transported into the sea by the Yellow River and the wind and waves on the sea surface.
Energy refinement and analysis of structures in the QM9 database via a highly accurate quantum chemical method
Authors: Kim Hyungjun , Park Ji Young , Choi Sunghwan
Published: July 2019

A wide variety of data-driven approaches have been introduced in the field of quantum chemistry. To extend the applicable range and improve the prediction power of those approaches, highly accurate quantum chemical benchmarks that cover extremely large chemical spaces are required. Here, we report ~134 k quantum chemical calculations performed with G4MP2, the fourth generation of the G-n series in which second-order perturbation theory is employed. A single composite method calculation executes several low-level calculations to reproduce the results of high-level ab initio calculations with the aim of saving computational costs. Therefore, our database reports the results of the various methods (e.g., density functional theory, Hartree-Fock, Møller–Plesset perturbation theory, and coupled-cluster theory). Additionally, we examined the structure information of both the QM9 and the revised databases via chemical graph analysis. Our database can be applied to refine and improve the quality of data-driven quantum chemical prediction. Furthermore, we reported the raw outputs of all calculations performed in this work for other potential applications.
Validation of an apnea and hypopnea detection algorithm implemented in implantable cardioverter defibrillators. The AIRLESS study
Authors: Defaye Pascal , Mendelson Monique , Tamisier Renaud , Jacon Peggy , Venier Sandrine , Arnol Nathalie , Pépin Jean-Louis
Published: July 2019

Diagnosis of sleep apnea (SA) using simple tools has the potential to improve the efficacy of cardiac implants in the prevention of cardiac arrhythmias. The aim of the present study was to validate a transthoracic impedance sensor for SA diagnosis in patients with cardiac implants. We compared the apnea-hypopnea index (AHI) obtained from polysomnography (AHIPSG) with the AHI obtained from autoscoring algorithms of the ApneaScan implantable impedance respiration sensor (AHIAS) three months after implantation of cardioverter-defibrillator (ICD) or cardiac resynchronization therapy-defibrillator (CRT-D) devices. Twenty-five patients with indications for implantation of ICD or CRT-D (INCEPTA; Boston Scientific) (24 men, 59.9 ± 14.4 years; LVEF 30.3 ± 6.4%; body mass index 25.9 ± 4.2 kg/m²) were included. Mean AHI-PSG was 21.9 ± 19.1 events/hr. A significant correlation was found between AHIPSG and AHIAS especially for the most severe SA (Spearman correlation: 0.71, p < 0.001). Intraclass Correlation Coefficient (was in the expected range: 0.67, 95% CI: 0.39–0.84. The mean bias was 5.4 events per hour (mean AHI: 23.3 ± 14.6 versus 29.7 ± 13.7 for AHI-PSG and AHI-AS, respectively). An optimal cutoff value for the AHIAS at 30 events/h was obtained from the Receiver Operator Characteristic (ROC) curve analysis, which yielded a sensitivity of 100%, a specificity of 80%, PPV = 67%, NPV = 100%. Using an advanced algorithm for autoscoring of transthoracic impedance included in ICDs is reliable to identify SA and has the potential to improve the management of patients with cardiac implants.
HyperFoods: Machine intelligent mapping of cancer-beating molecules in foods
Authors: Veselkov Kirill , Gonzalez Guadalupe , Aljifri Shahad , Galea Dieter , Mirnezami Reza , Youssef Jozef , Bronstein Michael , Laponogov Ivan
Published: July 2019

Recent data indicate that up-to 30–40% of cancers can be prevented by dietary and lifestyle measures alone. Herein, we introduce a unique network-based machine learning platform to identify putative food-based cancer-beating molecules. These have been identified through their molecular biological network commonality with clinically approved anti-cancer therapies. A machine-learning algorithm of random walks on graphs (operating within the supercomputing DreamLab platform) was used to simulate drug actions on human interactome networks to obtain genome-wide activity profiles of 1962 approved drugs (199 of which were classified as “anti-cancer” with their primary indications). A supervised approach was employed to predict cancer-beating molecules using these ‘learned’ interactome activity profiles. The validated model performance predicted anti-cancer therapeutics with classification accuracy of 84–90%. A comprehensive database of 7962 bioactive molecules within foods was fed into the model, which predicted 110 cancer-beating molecules (defined by anti-cancer drug likeness threshold of >70%) with expected capacity comparable to clinically approved anti-cancer drugs from a variety of chemical classes including flavonoids, terpenoids, and polyphenols. This in turn was used to construct a ‘food map’ with anti-cancer potential of each ingredient defined by the number of cancer-beating molecules found therein. Our analysis underpins the design of next-generation cancer preventative and therapeutic nutrition strategies.
Intracellular Trafficking Network and Autophagy of PHBHHx Nanoparticles and their Implications for Drug Delivery
Authors: Sun Xiangyu , Cheng Cheng , Zhang Jinxie , Jin Xing , Sun Shuqing , Mei Lin , Huang Laiqiang
Published: July 2019

3-hydroxybutyrate-co-3-hydroxyhexanoate (PHBHHx), which is naturally generated by biodegradable polyhydroxyalkanoates synthesized by bacteria, is an attractive material for drug delivery due to its controllable physical properties, non-toxicity, environmental friendliness, degradable properties and good biocompatibility. However, the intracellular trafficking network pathways, especially the autophagy mechanism of PHBHHx nanoparticles (NPs), have rarely been investigated. In this paper, we successfully prepared the NPs used solvent displacement method and investigated the autophagy pathways and other intracellular trafficking mechanisms based on NPs with the assistance of Rab proteins. We found that NPs were internalized in cells mainly via clathrin endocytosis and caveolin endocytosis. Beside the classical pathways, we discovered two new pathways: the micropinocytosis early endosome (EEs)-micropinocytosis-lysosome pathway and the EEs-liposome-lysosome pathway. NPs were delivered to cells through endocytosis recycling vesicles and GLUT4 exocytosis vesicles. Similar to other nanoparticles, NPs also induced intracellular autophagy and were then degraded via endolysosomal pathways. 3-MA and CQ were used as autophagy inhibitors to avoid the degradation of NPs through lysosomes by blocking endolysosomal pathways. Tumor volumes and weights were significantly decreased when autophagy inhibitors and chemical drugs packaged in NPs were cooperatively used.
MicroRNA profiling and identification of let-7a as a target to prevent chemotherapy-induced primordial follicles apoptosis in mouse ovaries
Authors: Alexandri C. , Stamatopoulos B. , Rothé F. , Bareche Y. , Devos M. , Demeestere I.
Published: July 2019

Cancer treatments as cyclophosphamide and its active metabolites are highly gonadotoxic leading to follicle apoptosis and depletion. Considering the risk of subsequent infertility, fertility preservation is recommended. Beside the germ cells and gametes cryopreservation options, ovarian pharmacological protection during treatment appears to be very attractive. Meanwhile, the advances in the field of oncology have brought microRNAs into spotlight as a potential feature of cancer treatment. Herein, we investigated miRNAs expressions in response to chemotherapy using postnatal-day-3 (PND3) mouse ovaries. Our results revealed that several miRNAs are differently expressed during chemotherapy exposure. Amongst them, let-7a was the most profoundly downregulated and targets genes involved in crucial cellular processes including apoptosis. Thus we developed a liposome-based system to deliver the let-7a mimic in whole PND3 ovariesin vitro. We showed that let-7a mimic prevented the upregulation of genes involved in cell death and reduced the chemotherapy-induced ovarian apoptosis, suggesting that it can be an interesting target to preserve ovarian function. However, its impact on subsequent follicular development has to be further elucidatedin vivousing an appropriate delivery system. In this study, we demonstrated that miRNA replacement approaches can be a useful tool to reduce chemotherapy-induced ovarian damage in the future.
CCR8 leads to eosinophil migration and regulates neutrophil migration in murine allergic enteritis
Authors: Blanco-Pérez Frank , Kato Yoichiro , Gonzalez-Menendez Irene , Laiño Jonathan , Ohbayashi Masaharu , Burggraf Manja , Krause Maren , Kirberg Jörg , Iwakura Yoichiro , Martella Manuela , Quintanilla-Martinez Leticia , Shibata Noriyuki , Vieths Stefan , Scheurer Stephan , Toda Masako
Published: July 2019

Allergic enteritis (AE) is a gastrointestinal form of food allergy. This study aimed to elucidate cellular and molecular mechanisms of AE using a murine model. To induce AE, BALB/c wild type (WT) mice received intraperitoneal sensitization with ovalbumin (an egg white allergen) plus ALUM and feeding an egg white (EW) diet. Microarray analysis showed enhanced gene expression of CC chemokine receptor (CCR) 8 and its ligand, chemokine CC motif ligand (CCL) 1 in the inflamed jejunum. Histological and FACS analysis showed that CCR8 knock out (KO) mice exhibited slightly less inflammatory features, reduced eosinophil accumulation but accelerated neutrophil accumulation in the jejunums, when compared to WT mice. The concentrations of an eosinophil chemoattractant CCL11 (eotaxin-1), but not of IL-5, were reduced in intestinal homogenates of CCR8KO mice, suggesting an indirect involvement of CCR8 in eosinophil accumulation in AE sites by inducing CCL11 expression. The potential of CCR8 antagonists to treat allergic asthma has been discussed. However, our results suggest that CCR8 blockade may promote neutrophil accumulation in the inflamed intestinal tissues, and not be a suitable therapeutic target for AE, despite the potential to reduce eosinophil accumulation. This study advances our knowledge to establish effective anti-inflammatory strategies in AE treatment.
Increased levels of the megakaryocyte and platelet expressed cysteine proteases stefin A and cystatin A prevent thrombosis
Authors: Mezzapesa Anna , Bastelica Delphine , Crescence Lydie , Poggi Marjorie , Grino Michel , Peiretti Franck , Panicot-Dubois Laurence , Dupont Annabelle , Valero René , Maraninchi Marie , Bordet Jean-Claude , Alessi Marie-Christine , Dubois Christophe , Canault Matthias
Published: July 2019

Increased platelet activity occurs in type 2 diabetes mellitus (T2DM) and such platelet dysregulation likely originates from altered megakaryopoiesis. We initiated identification of dysregulated pathways in megakaryocytes in the setting of T2DM. We evaluated through transcriptomic analysis, differential gene expressions in megakaryocytes from leptin receptor-deficient mice (db/db), exhibiting features of human T2DM, and control mice (db/+). Functional gene analysis revealed an upregulation of transcripts related to calcium signaling, coagulation cascade and platelet receptors in diabetic mouse megakaryocytes. We also evidenced an upregulation (7- to 9.7-fold) of genes encoding stefin A (StfA), the human ortholog of Cystatin A (CSTA), inhibitor of cathepsin B, H and L. StfA/CSTA was present in megakaryocytes and platelets and its expression increased during obesity and diabetes in rats and humans. StfA/CSTA was primarily localized at platelet membranes and granules and was released upon agonist stimulation and clot formation through a metalloprotease-dependent mechanism. StfA/CSTA did not affect platelet aggregation, but reduced platelet accumulation on immobilized collagen from flowing whole blood (1200 s−1). In-vivo, upon laser-induced vascular injury, platelet recruitment and thrombus formation were markedly reduced in StfA1-overexpressing mice without affecting bleeding time. The presence of CA-074Me, a cathepsin B specific inhibitor significantly reduced thrombus formation in-vitro and in-vivo in human and mouse, respectively. Our study identifies StfA/CSTA as a key contributor of platelet-dependent thrombus formation in both rodents and humans.
Study on the Relationship between Seed Absorbed Dose and Seed Composition of 252Cf Neutron Source Irradiated Bean Seed
Authors: Xu Dapeng , Li Yingguo , Yao Ze’en , Yin Yongzhi , Feng Huyuan , Wei Zheng
Published: July 2019

This study aims to further identify the biological effects of neutron-irradiated plants and provides insights into the mutation breeding of such plants. In this study, the neutron irradiation device designed by our institute was used to analyze the relationship between the seed components in different legume crops and their neutron absorption dose rate, fission gamma absorption dose rate, and induced gamma absorption dose rate. The results show that the effect sizes of the components on the neutron absorbed dose rate are as follows: ash > fat > moisture > carbohydrate > protein. The effect sizes of the components on the absorbed dose rate of fission gamma are as follows: ash > moisture > fat > carbohydrate > protein. There is a positive correlation between fission gamma absorbed dose rate and the weight of ash, water and fat, while a negative correlation with carbohydrate and protein. However, the linear relationship between each component and the absorbed dose rate of induced gamma is not significant, this needs to be identified by further researches. Based on the results of the present study, we conclude that the neutron absorbed dose can be calculated without taking into account the fat composition of bean crop seeds (except for soybean seeds) in the process of mutation breeding induced by radiation. In special cases where the accuracy requirement of the dose rate is not high, it is possible to use protein instead of legume crop seeds for neutron absorption dose calculations.
Common BACE2 Polymorphisms are Associated with Altered Risk for Alzheimer’s Disease and CSF Amyloid Biomarkers in APOE ε4 Non-Carriers
Authors: Huentelman Matt , De Both Matthew , Jepsen Wayne , Piras Ignazio S. , Talboom Joshua S. , Willeman Mari , Reiman Eric M. , Hardy John , Myers Amanda J.
Published: July 2019

It was recently suggested that beta-site amyloid precursor protein (APP)-cleaving enzyme 2 (BACE2) functions as an amyloid beta (Aβ)-degrading enzyme; in addition to its better understood role as an APP secretase. Due to this finding we sought to understand the possible genetic risk contributed by the BACE2 locus to the development of late-onset Alzheimer’s disease (AD). In this study, we report that common single nucleotide polymorphism (SNP) variation in BACE2 is associated with altered AD risk in apolipoprotein E gene (APOE) epsilon 4 variant (ε4) non-carriers. In addition, in ε4 non-carriers diagnosed with AD or mild cognitive impairment (MCI), SNPs within the BACE2 locus are associated with cerebrospinal fluid (CSF) levels of Aβ1-42. Further, SNP variants in BACE2 are also associated with BACE2 RNA expression levels suggesting a potential mechanism for the CSF Aβ1-42 findings. Lastly, overexpression of BACE2 in vitro resulted in decreased Aβ1-40 and Aβ1-42 fragments in a cell line model of Aβ production. These findings suggest that genetic variation at the BACE2 locus modifies AD risk for those individuals who don’t carry the ε4 variant of APOE. Further, our data indicate that the biological mechanism associated with this altered risk is linked to amyloid generation or clearance possibly through BACE2 expression changes.
Acoustic impact of a wave energy converter in Mediterranean shallow waters
Authors: Buscaino Giuseppa , Mattiazzo Giuliana , Sannino Gianmaria , Papale Elena , Bracco Giovanni , Grammauta Rosario , Carillo Adriana , Kenny Jose Maria , De Cristofaro Norma , Ceraulo Maria , Mazzola Salvatore
Published: July 2019

In this study, underwater noise from a full-scale wave energy converter system (ISWEC), installed on the coast of Pantelleria Island (central Mediterranean Sea), was characterized. The noise was measured using an autonomous acoustic recorder anchored to the sea bottom 40 m from the ISWEC hull. Acoustic monitoring continued for 15 months, starting 7 months before (PRE), 2 months during (INST) and 6 months after the ISWEC installation (POST). The levels of noise, assessed with power spectrum density and octave and third-octave band sound pressure levels (BSPLs), were higher during the POST period than during the PRE period at lower frequencies up to 4 kHz and increased with wave height. During the ISWEC activation for energy production (POST_ON) in the wave height range 1–2.9 m, the BSPLs increased much more at lower frequencies up to 4 kHz (the median BSPLs at 63 Hz for the PRE, POST, and POST_ON conditions were 73, 106, and 126 dB re 1μPa, respectively). Considering the biophonies that make up the soundscape of the area, we examined the possible masking of fish choruses due to ISWEC noise and highlighted that at a distance of 1000 m, the 800 Hz peak frequency was 10 dB above the ISWEC signal. Within this distance from ISWEC, a possible masking effect is supposed.
A Novel Etchant System for Orthodontic Bracket Bonding
Authors: Ibrahim A. I. , Thompson V. P. , Deb S.
Published: July 2019

Orthodontic treatment is widely used to correct irregular teeth and/or jaw discrepancies to improve oral function and facial aesthetics. However, it is frequently associated with enamel damage that include chipping, demineralisation, and white spot formation. So far, current bonding systems that can maintain shear bond strengths (SBS) suitable for clinical performance are unable to limit enamel demineralisation, adhesive remnants and damage caused on removal of brackets after treatment. This study reports a novel “safe enamel etch” clinically viable procedure that was accomplished via application of novel etchant pastes developed with β-tricalcium phosphate and monocalcium phosphate monohydrate powders mixed with citric acid (5 M) or phosphoric acid (37% PA) to yield BCA and BPA etchants respectively. Although enamel etched with clinically used PA gel yielded higher SBS than the BCA/BPA etchants, it exhibited greater adhesive remnants with evidence of enamel damage. In contrast, the experimental etchants resulted in unblemished enamel surfaces with zero or minimal adhesive residue and clinically acceptable SBS. Furthermore, the BPA etchant caused lower enamel decalcification with extensive calcium-phosphate precipitation. The study conclusively showed that BPA facilitated in vitro enamel adhesion without detrimental effects of the aggressive PA gel with potential for remineralisation and saving time at the post-debonding step.
Impacts of the synthetic androgen Trenbolone on gonad differentiation and development – comparisons between three deeply diverged anuran families
Authors: Rozenblut-Kościsty Beata , Ogielska Maria , Hahn Juliane , Kleemann Denise , Kossakowski Ronja , Tamschick Stephanie , Schöning Viola , Krüger Angela , Lutz Ilka , Lymberakis Petros , Kloas Werner , Stöck Matthias
Published: July 2019

Using a recently developed approach for testing endocrine disruptive chemicals (EDCs) in amphibians, comprising synchronized tadpole exposure plus genetic and histological sexing of metamorphs in a flow-through-system, we tested the effects of 17β-Trenbolone (Tb), a widely used growth promoter in cattle farming, in three deeply diverged anuran families: the amphibian model species Xenopus laevis (Pipidae) and the non-models Bufo(tes) viridis (Bufonidae) and Hyla arborea (Hylidae). Trenbolone was applied in three environmentally and/or physiologically relevant concentrations (0.027 µg/L (10−10 M), 0.27 µg/L (10−9 M), 2.7 µg/L (10−8 M)). In none of the species, Tb caused sex reversals or masculinization of gonads but had negative species-specific impacts on gonad morphology and differentiation after the completion of metamorphosis, independently of genetic sex. In H. arborea and B. viridis, mounting Tb-concentration correlated positively with anatomical abnormalities at 27 µg/L (10−9 M) and 2.7 µg/L (10−8 M), occurring in X. laevis only at the highest Tb concentration. Despite anatomical aberrations, histologically all gonadal tissues differentiated seemingly normally when examined at the histological level but at various rates. Tb-concentration caused various species-specific mortalities (low in Xenopus, uncertain in Bufo). Our data suggest that deep phylogenetic divergence modifies EDC-vulnerability, as previously demonstrated for Bisphenol A (BPA) and Ethinylestradiol (EE2).
Machine learning approaches to predict lupus disease activity from gene expression data
Authors: Kegerreis Brian , Catalina Michelle D. , Bachali Prathyusha , Geraci Nicholas S. , Labonte Adam C. , Zeng Chen , Stearrett Nathaniel , Crandall Keith A. , Lipsky Peter E. , Grammer Amrie C.
Published: July 2019

The integration of gene expression data to predict systemic lupus erythematosus (SLE) disease activity is a significant challenge because of the high degree of heterogeneity among patients and study cohorts, especially those collected on different microarray platforms. Here we deployed machine learning approaches to integrate gene expression data from three SLE data sets and used it to classify patients as having active or inactive disease as characterized by standard clinical composite outcome measures. Both raw whole blood gene expression data and informative gene modules generated by Weighted Gene Co-expression Network Analysis from purified leukocyte populations were employed with various classification algorithms. Classifiers were evaluated by 10-fold cross-validation across three combined data sets or by training and testing in independent data sets, the latter of which amplified the effects of technical variation. A random forest classifier achieved a peak classification accuracy of 83 percent under 10-fold cross-validation, but its performance could be severely affected by technical variation among data sets. The use of gene modules rather than raw gene expression was more robust, achieving classification accuracies of approximately 70 percent regardless of how the training and testing sets were formed. Fine-tuning the algorithms and parameter sets may generate sufficient accuracy to be informative as a standalone estimate of disease activity.
Robust Surface-Engineered Tape-Cast and Extrusion Methods to Fabricate Electrically-Conductive Poly(vinylidene fluoride)/Carbon Nanotube Filaments for Corrosion-Resistant 3D Printing Applications
Authors: Almazrouei Asma , Susantyoko Rahmat Agung , Wu Chieh-Han , Mustafa Ibrahim , Alhammadi Ayoob , Almheiri Saif
Published: July 2019

We developed a poly(vinylidene fluoride)/carbon nanotube (PVDF-MWCNT) filament as a feed for printing of electrically-conductive and corrosion-resistant functional material by fused filament fabrication (FFF). Using an environment-friendly procedure to fabricate PVDF-MWCNT filament, we achieved the best reported electrical conductivity of printable PVDF-MWCNT filament of 28.5 S cm−1 (90 wt% PVDF and 10 wt% CNT). The PVDF-MWCNT filaments are chemically stable in acid, base, and salt solution, with no significant changes in electrical conductivity and mass of the filaments. Our processing method is robust and allow a uniform mixture of PVDF and CNT with a wide range of CNT percentage up to 99.9%. We demonstrated the printing of PVDF-MWCNT filaments to create 3D shapes; printed using a low-cost commercial consumer-grade FFF 3D printer. We found many adjustments of printer parameters are needed to print filament with CNT content >10 wt%, but easier printing for CNT content ≤10 wt%. Since this was due to printer limitation, we believed that PVDF-MWCNT with higher CNT percentage (to a certain limit) and larger electrical conductivity could be printed with a custom-built printer (for example stronger motor). PVDF-MWCNT filament shows higher electrical conductivity (28.5 S cm−1) than compressed composite (8.8 S cm−1) of the same 10 wt% of CNT, due to more alignment of CNT in the longitudinal direction of the extruded filament. Printable PVDF-MWCNT-Fe2O3 (with a functional additive of Fe2O3) showed higher electrical conductivity in the longitudinal direction at the filament core (42 S cm−1) compared to that in the longitudinal direction at the filament shell (0.43 S cm−1) for sample with composition of 60 wt% PVDF, 20 wt% CNT, and 20 wt% Fe2O3, due to extrusion skin effect with segregation of electrically insulating Fe2O3 at the shell surface of PVDF-MWCNT-Fe2O3.
Conditional loss of ERK1 and ERK2 results in abnormal placentation and delayed parturition in the mouse
Authors: Brown Jessica L. , Sones Jennifer L. , Angulo Cynthia N. , Abbott Keelin , Miller Andrew D. , Boehm Ulrich , Roberson Mark S.
Published: July 2019

Extracellular-signal-regulated kinases (ERK) 1 and 2 regulate many aspects of the hypothalamic-pituitary-gonadal axis. We sought to understand the role of ERK1/2 signaling in cells expressing a Cre allele regulated by the endogenous GnRHR promoter (GRIC-ERKdko). Adult female GRIC-ERKdko mice were hypogonadotropic and anovulatory. Gonadotropin administration and mating led to pregnancy in one-third of the ERKdko females. Litters from ERKdko females and pup weights were reduced coincident with delayed parturition and 100% neonatal mortality. Based on this, we examined Cre expression in implantation sites as a potential mechanism. GnRHR mRNA levels at e10.5 and e12.5 were comparable to pituitary levels from adult female mice at proestrus and GnRHR mRNA in decidua was enriched compared to whole implantation site. In vivo studies confirmed recombination in decidua, and GRIC-ERKdko placentas showed reduced ERK2 expression. Histopathology revealed abnormalities in placental architecture in the GRIC-ERKdko animals. Regions of apoptosis at the decidual/uterine interface at e18.5 were observed in control animals but apoptotic tone in these regions was reduced in ERKdko animals. These studies support a potential model of ERK-dependent signaling within the implantation site leading to loss of placental architecture and mis-regulation of apoptotic events at parturition occurring coincident with prolonged gestation and neonatal mortality.
Remote targeted implantation of sound-sensitive biodegradable multi-cavity microparticles with focused ultrasound
Authors: Su Xiaoqian , Thomas Reju George , Bharatula Lakshmi Deepika , Kwan James J.
Published: July 2019

Ultrasound-enhanced drug delivery has shown great promise in providing targeted burst release of drug at the site of the disease. Yet current solid ultrasound-responsive particles are non-degradable with limited potential for drug-loading. Here, we report on an ultrasound-responsive multi-cavity poly(lactic-co-glycolic acid) microparticle (mcPLGA MP) loaded with rhodamine B (RhB) with or without 4′,6-diamidino-2-phenylindole (DAPI) to represent small molecule therapeutics. After exposure to high intensity focused ultrasound (HIFU), these delivery vehicles were remotely implanted into gel and porcine tissue models, where the particles rapidly released their payload within the first day and sustained release for at least seven days. RhB-mcPLGA MPs were implanted with HIFU into and beyond the sub-endothelial space of porcine arteries without observable damage to the artery. HIFU also guided the location of implantation; RhB-mcPLGA MPs were only observed at the focus of the HIFU away from the direction of ultrasound. Once implanted, DAPI co-loaded RhB-mcPLGA MPs released DAPI into the arterial wall, staining the nucleus of the cells. Our work shows the potential for HIFU-guided implantation of drug-loaded particles as a strategy to improve the local and sustained delivery of a therapeutic for up to two weeks.
Interfacial Effect-Based Quantification of Droplet Isothermal Nucleic Acid Amplification for Bacterial Infection
Authors: Ulep Tiffany-Heather , Day Alexander S. , Sosnowski Katelyn , Shumaker Alexa , Yoon Jeong-Yeol
Published: July 2019

Bacterial infection is a widespread problem in humans that can potentially lead to hospitalization and morbidity. The largest obstacle for physicians/clinicians is the time delay in accurately identifying infectious bacteria, especially their sub-species, in order to adequately treat and diagnose such infected patients. Loop-mediated amplification (LAMP) is a nucleic acid amplification method that has been widely used in diagnostic applications due to its simplicity of constant temperature, use of up to 4 to 6 primers (rendering it highly specific), and capability of amplifying low copies of target sequences. Use of interfacial effect-based monitoring is expected to dramatically shorten the time-to-results of nucleic acid amplification techniques. In this work, we developed a LAMP-based point-of-care platform for detection of bacterial infection, utilizing smartphone measurement of contact angle from oil-immersed droplet LAMP reactions. Whole bacteria (Escherichia coli O157:H7) were assayed in buffer as well as 5% diluted human whole blood. Monitoring of droplet LAMP reactions was demonstrated in a three-compartment, isothermal proportional-integrated-derived (PID)-controlled chip. Smartphone-captured images of droplet LAMP reactions, and their contact angles, were evaluated. Contact angle decreased substantially upon target amplification in both buffer and whole blood samples. In comparison, no-target control (NTC) droplets remained stable throughout the 30 min isothermal reactions. These results were explained by the pre-adsorption of plasma proteins to an oil-water interface (lowering contact angle), followed by time-dependent amplicon formation and their preferential adsorption to the plasma protein-occupied oil-water interface. Time-to-results was as fast as 5 min, allowing physicians to quickly make their decision for infected patients. The developed assay demonstrated quantification of bacteria concentration, with a limit-of-detection at 102 CFU/μL for buffer samples, and binary target or no-target identification with a limit-of-detection at 10 CFU/μL for 5% diluted whole blood samples.
iTRAQ-based quantitative proteomic analysis of salt stress in Spica Prunellae
Authors: Liu Zixiu , Zou Lisi , Chen Cuihua , Zhao Hui , Yan Ying , Wang Chengcheng , Liu Xunhong
Published: July 2019

Spica Prunellae is an important Chinese herbal medicine. Because of its good curative effect on various diseases, this herb is consumed in large quantities in clinical applications. The metabolites of Spica Prunellae are known to change under salt stress; however, the difference in protein levels of Spica Prunellae between saline and normal conditions is unclear. In this study, we used proteomics techniques to identify differentially expressed proteins in Spica Prunellae under different saline conditions. (iTRAQ) MS/MS was used to detect statistically significant changes in protein between salt stress and normal conditions. Ultimately, we detected 1,937 proteins, 89 of which were detected in two different comparison. Based on GO, STRING and KEGG analyses, 35 significantly differentially expressed proteins were selected for further analysis. The results of functional and signal pathway analyses indicated that the cellular protein and carbohydrate metabolism of Spica Prunellae was weaker, calcium ion transport was higher, photosynthesis was higher, and protein production was faster under saline conditions than under normal conditions. This study provides useful information for studying the causes of differences in secondary metabolites in Spica Prunellae under salt stress and the protein mechanisms related to their quality.
Functional and metabolic impairment in cigarette smoke-exposed macrophages is tied to oxidative stress
Authors: Aridgides Daniel S. , Mellinger Diane L. , Armstrong David A. , Hazlett Haley F. , Dessaint John A. , Hampton Thomas H. , Atkins Graham T. , Carroll James L. , Ashare Alix
Published: July 2019

Cigarette smoke inhalation exposes the respiratory system to thousands of potentially toxic substances and causes chronic obstructive pulmonary disease (COPD). COPD is characterized by cycles of inflammation and infection with a dysregulated immune response contributing to disease progression. While smoking cessation can slow the damage in COPD, lung immunity remains impaired. Alveolar macrophages (AMΦ) are innate immune cells strategically poised at the interface between lungs, respiratory pathogens, and environmental toxins including cigarette smoke. We studied the effects of cigarette smoke on model THP-1 and peripheral blood monocyte derived macrophages, and discovered a marked inhibition of bacterial phagocytosis which was replicated in primary human AMΦ. Cigarette smoke decreased AMΦ cystic fibrosis transmembrane conductance regulator (CFTR) expression, previously shown to be integral to phagocytosis. In contrast to cystic fibrosis macrophages, smoke-exposed THP-1 and AMΦ failed to augment phagocytosis in the presence of CFTR modulators. Cigarette smoke also inhibited THP-1 and AMΦ mitochondrial respiration while inducing glycolysis and reactive oxygen species. These effects were mitigated by the free radical scavenger N-acetylcysteine, which also reverted phagocytosis to baseline levels. Collectively these results implicate metabolic dysfunction as a key factor in the toxicity of cigarette smoke to AMΦ, and illuminate avenues of potential intervention.
Intelligent Diagnostic Prediction and Classification System for Chronic Kidney Disease
Authors: Elhoseny Mohamed , Shankar K. , Uthayakumar J.
Published: July 2019

At present times, healthcare systems are updated with advanced capabilities like machine learning (ML), data mining and artificial intelligence to offer human with more intelligent and expert healthcare services. This paper introduces an intelligent prediction and classification system for healthcare, namely Density based Feature Selection (DFS) with Ant Colony based Optimization (D-ACO) algorithm for chronic kidney disease (CKD). The proposed intelligent system eliminates irrelevant or redundant features by DFS in prior to the ACO based classifier construction. The proposed D-ACO framework three phases namely preprocessing, Feature Selection (FS) and classification. Furthermore, the D-ACO algorithm is tested using benchmark CKD dataset and the performance are investigated based on different evaluation factors. Comparing the D-ACO algorithm with existing methods, the presented intelligent system outperformed the other methodologies with a significant improvisation in classification accuracy using fewer features.
Xylem cavitation susceptibility and refilling mechanisms in olive trees infected by Xylella fastidiosa
Authors: Sabella Erika , Aprile Alessio , Genga Alessandra , Siciliano Tiziana , Nutricati Eliana , Nicolì Francesca , Vergine Marzia , Negro Carmine , De Bellis Luigi , Luvisi Andrea
Published: July 2019

In olive trees, Xylella fastidiosa colonizes xylem vessels and compromises water transport causing the olive quick decline syndrome (OQDS). The loss of hydraulic conductivity could be attributed to vessel occlusions induced both by the bacteria biofilm and by plant responses (tyloses, gums, etc.) that could trigger embolism. The ability of the infected plants to detect embolism and to respond, by activating mechanisms to restore the hydraulic conductivity, can influence the severity of the disease symptomatology. In order to investigate these mechanisms in the X. fastidiosa-resistant olive cultivar Leccino and in the susceptible Cellina di Nardò, sections of healthy olive stems were analysed by laser scanning microscope to calculate the cavitation vulnerability index. Findings indicated that the cultivar Leccino seems to be constitutively less susceptible to cavitation than the susceptible one. Among the vascular refilling mechanisms, starch hydrolysis is a well-known strategy to refill xylem vessels that suffered cavitation and it is characterized by a dense accumulation of starch grains in the xylem parenchima; SEM-EDX analysis of stem cross-sections of infected plants revealed an aggregation of starch grains in the Leccino xylem vessels. These observations could indicate that this cultivar, as well as being anatomically less susceptible to cavitation, it also could be able to activate more efficient refilling mechanisms, restoring vessel’s hydraulic conductivity. In order to verify this hypothesis, we analysed the expression levels of some genes belonging to families involved in embolism sensing and refilling mechanisms: aquaporins, sucrose transporters, carbohydrate metabolism and enzymes related to starch breakdown, alpha and beta-amylase. The obtained genes expression patterns suggested that the infected plants of the cultivar Leccino strongly modulates the genes involved in embolism sensing and refilling.
FGF family members differentially regulate maturation and proliferation of stem cell-derived astrocytes
Authors: Savchenko Ekaterina , Teku Gabriel N. , Boza-Serrano Antonio , Russ Kaspar , Berns Manon , Deierborg Tomas , Lamas Nuno J. , Wichterle Hynek , Rothstein Jeffrey , Henderson Christopher E. , Vihinen Mauno , Roybon Laurent
Published: July 2019

The glutamate transporter 1 (GLT1) is upregulated during astrocyte development and maturation in vivo and is vital for astrocyte function. Yet it is expressed at low levels by most cultured astrocytes. We previously showed that maturation of human and mouse stem cell-derived astrocytes – including functional glutamate uptake – could be enhanced by fibroblast growth factor (FGF)1 or FGF2. Here, we examined the specificity and mechanism of action of FGF2 and other FGF family members, as well as neurotrophic and differentiation factors, on mouse embryonic stem cell-derived astrocytes. We found that some FGFs – including FGF2, strongly increased GLT1 expression and enhanced astrocyte proliferation, while others (FGF16 and FGF18) mainly affected maturation. Interestingly, BMP4 increased astrocytic GFAP expression, and BMP4-treated astrocytes failed to promote the survival of motor neurons in vitro. Whole transcriptome analysis showed that FGF2 treatment regulated multiple genes linked to cell division, and that the mRNA encoding GLT1 was one of the most strongly upregulated of all astrocyte canonical markers. Since GLT1 is expressed at reduced levels in many neurodegenerative diseases, activation of this pathway is of potential therapeutic interest. Furthermore, treatment with FGFs provides a robust means for expansion of functionally mature stem cell-derived astrocytes for preclinical investigation.