bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical evidence of acupuncture and moxibustion for irritable bowel syndrome: A systematic review and meta-analysis of randomized controlled trials
Authors: Yang Yuanming , Rao Kehan , Zhan Kai , Shen Min , Zheng Huan , Qin Shumin , Wu Haomeng , Bian Zhaoxiang , Huang Shaogang
Published: November 2022

Background

Acupuncture and moxibustion have been widely used in the treatment of Irritable Bowel Syndrome (IBS). But the evidence that acupuncture and moxibustion for IBS reduction of symptom severity and abdominal pain, and improvement of quality of life is scarce.

Methods

PubMed, Embase, Cochrane Library, Web of Science, Chinese National Knowledge Infrastructure (CNKI), Chinese Scientific Journals Database (VIP), Wanfang Database, China Biomedical Literature Service System (SinoMed), and unpublished sources were searched from inception until June 30, 2022. The quality of RCTs was assessed with the Cochrane Collaboration risk of bias tool. The strength of the evidence was evaluated with the Grading of Recommendations Assessment, Development and Evaluation system (GRADE). Trial sequential analysis (TSA) was conducted to determine whether the participants in the included trials had reached optimal information size and whether the cumulative data was adequately powered to evaluate outcomes.

Results

A total of 31 RCTs were included. Acupuncture helped reduce the severity of symptoms more than pharmaceutical drugs (MD, −35.45; 95% CI, −48.21 to −22.68; I2 = 71%). TSA showed the cumulative Z score crossed O'Brien-Fleming alpha-spending significance boundaries. Acupuncture wasn't associated with symptom severity reduction (SMD, 0.03, 95% CI, −0.25 to 0.31, I2 = 46%), but exhibited therapeutic benefits on abdominal pain (SMD, −0.24; 95% CI, −0.48 to −0.01; I2 = 8%) compared to sham acupuncture. Moxibustion show therapeutic benefits compared to sham moxibustion on symptom severity (SMD, −3.46, 95% CI, −5.66 to −1.27, I2 = 95%) and abdominal pain (SMD, −2.74, 95% CI, −4.81 to −0.67, I2 = 96%). Acupuncture (SMD, −0.46; 95% CI, −0.68 to −0.24; I2 = 47%) and the combination of acupuncture and moxibustion (SMD, −2.00; 95% CI, −3.04 to −0.96; I2 = 90%) showed more benefit for abdominal pain compared to pharmacological medications as well as shams. Acupuncture (MD, 4.56; 95% CI, 1.46–7.67; I2 = 79%) and moxibustion (MD, 6.97; 95% CI, 5.78–8.16; I2 = 21%) were more likely to improve quality of life than pharmaceutical drugs.

Conclusion

Acupuncture and/or moxibustion are beneficial for symptom severity, abdominal pain and quality of life in IBS. However, in sham control trials, acupuncture hasn't exhibited robust and stable evidence, and moxibustion's results show great heterogeneity. Hence, more rigorous sham control trials of acupuncture or moxibustion are necessary.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=262118, identifier CRD42021262118.
Development and validation of a nomogram for the early prediction of acute kidney injury in hospitalized COVID-19 patients
Authors: Wang Congjie , Sun Huiyuan , Li Xinna , Wu Daoxu , Chen Xiaoqing , Zou Shenchun , Jiang Tingshu , Lv Changjun
Published: November 2022

Introduction

Acute kidney injury (AKI) is a prevalent complication of coronavirus disease 2019 (COVID-19) and is closely linked with a poorer prognosis. The aim of this study was to develop and validate an easy-to-use and accurate early prediction model for AKI in hospitalized COVID-19 patients.

Methods

Data from 480 COVID-19-positive patients (336 in the training set and 144 in the validation set) were obtained from the public database of the Cancer Imaging Archive (TCIA). The least absolute shrinkage and selection operator (LASSO) regression method and multivariate logistic regression were used to screen potential predictive factors to construct the prediction nomogram. Receiver operating curves (ROC), calibration curves, as well as decision curve analysis (DCA) were adopted to assess the effectiveness of the nomogram. The prognostic value of the nomogram was also examined.

Results

A predictive nomogram for AKI was developed based on arterial oxygen saturation, procalcitonin, C-reactive protein, glomerular filtration rate, and the history of coronary artery disease. In the training set, the nomogram produced an AUC of 0.831 (95% confidence interval [CI]: 0.774–0.889) with a sensitivity of 85.2% and a specificity of 69.9%. In the validation set, the nomogram produced an AUC of 0.810 (95% CI: 0.737–0.871) with a sensitivity of 77.4% and a specificity of 78.8%. The calibration curve shows that the nomogram exhibited excellent calibration and fit in both the training and validation sets. DCA suggested that the nomogram has promising clinical effectiveness. In addition, the median length of stay (m-LS) for patients in the high-risk group for AKI (risk score ≥ 0.122) was 14.0 days (95% CI: 11.3–16.7 days), which was significantly longer than 8.0 days (95% CI: 7.1–8.9 days) for patients in the low-risk group (risk score <0.122) (hazard ratio (HR): 1.98, 95% CI: 1.55–2.53, p < 0.001). Moreover, the mortality rate was also significantly higher in the high-risk group than that in the low-risk group (20.6 vs. 2.9%, odd ratio (OR):8.61, 95%CI: 3.45–21.52).

Conclusions

The newly constructed nomogram model could accurately identify potential COVID-19 patients who may experience AKI during hospitalization at the very beginning of their admission and may be useful for informing clinical prognosis.
The effect of different types of physical activity on cognitive reaction time in older adults in China
Authors: Liu Yujie , Hou Xiao , Tang Zhengyan , Zhang Hanyue , Liu Jingmin
Published: November 2022

Introduction

Aging is not only reflected in the degeneration of physiological functions but is also embodied in the decline of psychological and cognitive functions. The decline of cognitive function can reduce the quality of life in older adults, and even potentially cause Alzheimer's disease, which may lead to a heavy burden on patients, families, and society. The purpose of this study was to investigate the effects of physical activity (PA) on cognitive reaction time in older adults.

Methods

A cross-sectional survey design was used in this study. A total of 839 elderly subjects were recruited from Beijing and Shanghai. In total, 792 subjects met the inclusion criteria (age > 60 years, without disability, speech, and hearing impairment), including 384 men (age:67.7 ± 5.7 years) and 408 women (age: 68.2 ± 5.8 years). The PA was assessed by the Physical Activity Scale for the Elderly (PASE). All kinds of PA were divided into three levels: “Low” (< 50% average score), “Moderate” (50–150% average score), and “High” (>150% average score). The reaction time of subjects was measured by the selective reaction tester (Model: CSTF-XF, TFHT, Beijing, China).

Results

For leisure-time PA, the results showed that the cognitive reaction time of older adults in the “Low” group (1.11 ± 0.32 s) was significantly longer than that in the “Moderate” group (1.05 ± 0.30 s, p < 0.01) and the “High” group (0.99 ± 0.28 s, p < 0.01). For housework PA, there was no significant difference in the cognitive reaction time among the three groups (“Low”: 1.09 ± 0.31 s; “Moderate”: 1.07 ± 0.31 s; “High”: 1.05 ± 0.28 s, p > 0.05). For work-related PA, the results showed that the cognitive reaction time of older adults in the “Low” group (1.09 ± 0.30 s) was significantly longer than that in the “High” group (0.99 ± 0.28 s) and the “Moderate” group (1.03 ± 0.32 s, p < 0.01).

Conclusion

The PA has a positive effect on reducing cognitive reaction time in older adults. It is recommended that older adults maintain a moderate level of leisure PA and work-related PA to delay the decline in cognitive reaction time.
Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region
Authors: Crispell Gary , Williams Kelly , Zielinski Eric , Iwami Akira , Homas Zachary , Thomas Karen
Published: November 2022

Introduction

Japanese encephalitis virus (JEV) is a mosquito-borne viral pathogen, which is becoming a growing public health concern throughout the Indo-Pacific. Five genotypes of JEV have been identified. Current vaccines are based on genotype III and provide a high degree of protection for four of the five known genotypes.

Methods

RT-PCR, Magpix, Twist Biosciences Comprehensive Viral Research Panel (CVRP), and SISPA methods were used to detect JEV from mosquito samples collected in South Korea during 2021. These methods were compared to determine which method would be most effective for biosurveillance in the Indo-Pacific region.

Results

Our data showed that RT-PCR, Twist CVRP, and SISPA methods were all able to detect JEV genotype I, however, the proprietary Magpix panel was only able to detect JEV genotype III. Use of minION sequencing for pathogen detection in arthropod samples will require further method development.

Conclusion

Biosurveillance of vectorborne pathogens remains an area of concern throughout the Indo-Pacific. RT-PCR was the most cost effective method used in the study, but TWIST CVRP allows for the identification of over 3,100 viral genomes. Further research and comparisons will be conducted to ensure optimal methods are used for large scale biosurveillance.
“Go with the Flo”: Conducting rapid research on prenatal stress following Hurricane Florence as participant observers
Authors: Howells Michaela , Dancause Kelsey
Published: November 2022

In this article, we explore the challenges of conceptualizing, designing, and establishing a rapid research agenda as a local researcher following a disaster. We share what we learned while developing and implementing this rapid study and explore the challenges shaped by time pressures, our local context, and resource availability. We identify four core challenges, experienced conducting rapid research, and provide suggestions to overcome these challenges. Our goal is to provide insight to undergraduates, graduate students, and professionals who are considering rapid research inside or outside their own communities.
Correlates of vaccine protection against Mycobacterium avium sub-species paratuberculosis infection revealed in a transcriptomic study of responses in Gudair® vaccinated sheep
Authors: Purdie Auriol C. , Plain Karren M. , Pooley Hannah , Begg Douglas J. , de Silva Kumudika , Whittington Richard J.
Published: November 2022

A critical hindrance in the development of effective vaccine strategies to combat infectious disease is lack of knowledge about correlates of protection and of the host responses necessary for successful adaptive immunity. Often vaccine formulations are developed by stepwise experimentation, with incomplete investigation of the fundamental mechanisms of protection. Gudair®is a commercially available vaccine registered for use in sheep and goats for controlling spread ofMycobacterium aviumsub-speciesparatuberculosis(MAP) infections and reduces mortality by up to 90%. Here, using an experimental infection model in sheep, we have utilized a transcriptomics approach to identify white blood cell gene expression changes in vaccinated, MAP-exposed Merino sheep with a protective response in comparison to those vaccinated animals that failed to develop immunity to MAP infection. This methodology facilitated an overview of gene-associated functional pathway adaptations using anin-silicoanalysis approach. We identified a group of genes that were activated in the vaccine-protected animals and confirmed stability of expression in samples obtained from naturally exposed commercially maintained sheep. We propose these genes as correlates of vaccine induced protection.
Homing of radiolabelled xenogeneic equine peripheral blood-derived MSCs towards a joint lesion in a dog
Authors: Beerts Charlotte , Pauwelyn Glenn , Depuydt Eva , Xu Yangfeng , Saunders Jimmy H. , Peremans Kathelijne , Spaas Jan H.
Published: November 2022

Osteoarthritis (OA) is a highly prevalent condition in dogs, causing a substantial reduction in quality of life and welfare of the animals. Current disease management focusses on pain relief but does not stop the progression of the disease. Therefore, mesenchymal stem cells (MSCs) could offer a promising disease modifying alternative. However, little is known about the behavior and the mode of action of MSCs following their administration. In the current case report, 99mTechnetium labelled xenogeneic equine peripheral blood-derived MSCs were intravenously injected in a 9 year old dog suffering from a natural occurring cranial cruciate ligament rupture. The biodistribution of the MSCs was evaluated during a 6-h follow-up period, using a full body scintigraphy imaging technique. No clinical abnormalities or ectopic tissue formations were detected throughout the study. A radiopharmaceutical uptake was present in the liver, heart, lung, spleen, kidneys and bladder of the dog. Furthermore, homing of the radiolabelled MSCs to the injured joint was observed, with 40.61 % higher uptake in the affected joint in comparison with the healthy contralateral joint. Finally, a local radioactive hotspot was seen at a part of the tail of the dog that had been injured recently. The current study is the first to confirm the homing of xenogeneic MSCs to a naturally occurring joint lesion after IV administration.
Addition of a combination of creatine, carnitine, and choline to a commercial diet increases postprandial plasma creatine and creatinine concentrations in adult dogs
Authors: Banton Sydney , Braun Ulrike , Squires E. James , Shoveller Anna K.
Published: November 2022

Creatine is a nitrogenous compound essential for cellular energy homeostasis found in animal protein; however, when heat-processed for pet food, creatine is degraded to creatinine, which is not metabolically active and excreted in urine. The objective of the present investigation was to define the postprandial plasma creatine and creatinine response in dogs fed a commercial diet (CON) formulated for adult dogs, top-dressed with a combination of creatine (9.6 g/kg dry matter, DM), carnitine (2.13 g/kg DM) and choline (0.24 g/kg DM; CCC), methionine (2.6 g/kg DM; MET), or taurine (0.7 g/kg DM; TAU). Eight adult Beagles were fed one of the four diets for 7 days in a Latin Square design with no washout period. On day 7, cephalic catheters were placed and blood samples were collected before being fed (fasted) and up to 6 h post-meal. Creatine and creatinine were analyzed using HPLC and data analyzed using PROC GLIMMIX in SAS. Plasma creatine concentrations were higher in dogs fed CCC (103 ± 10 μmol/L) compared to MET (72 ± 7 μmol/L) at fasted (P < 0.05) and higher compared to all other treatments from 15 to 360 min post-meal (P < 0.05). Plasma creatinine concentrations were higher in dogs fed CCC from 60 to 180 min compared to all other treatments. These data suggest that when creatine, carnitine and choline are top-dressed for 7 days, plasma creatine is rapidly absorbed and remains elevated up to 6 h post-meal. This may have implications for energy metabolism and should be considered when using creatinine as a diagnostic tool in dogs.
Knowledge, attitudes and practices of government animal health workers on antibiotic use and antibiotic resistance in Timor-Leste
Authors: Ting Shawn , Pereira Abrao , Alves Amalia , Vong da Silva Paulo Gabriel , Dos Santos Cristibela , Davis Steven , Sidjabat Hanna E. , Yan Jennifer , Francis Joshua R. , Bendita da Costa Jong Joanita , Barnes Tamsin S.
Published: November 2022

Introduction

Antibiotic resistance is a global health threat, and there is growing concern on the inappropriate use of antibiotics in the livestock sector especially in low and middle income countries. The purpose of the study was to understand the knowledge, attitudes and practices on antibiotic use and antibiotic resistance of government animal health workers in Timor-Leste.

Method

A cross-sectional survey using a census approach was conducted between August 2021 and January 2022 focusing on government animal health workers involved in field work and access to antibiotics. Interviews were face-to-face in the local Tetun language. Descriptive and regression analysis informed by causal diagrams were performed.

Result

The study found poor knowledge of antibiotics among participants, with only 8.0% (13/162) able to correctly answer questions on how antibiotics worked. Knowledge of antibiotic resistance was poor as only 29.0% (47/162) of participants had heard of antibiotic resistance and were able to accurately identify that it made antibiotics less effective. Knowledge of antibiotics and knowledge of antibiotic resistance were crudely associated with being a veterinary technician and having university education. Attitude scores were positively influenced by knowledge of antibiotics and antibiotic resistance. Antibiotics were most commonly used in pigs, cattle and buffalo, with oxytetracycline being the most commonly used antibiotics in pigs and chicken. However, most participants reported a lack in supply of this antibiotic (137/162, 78.4%) and other antibiotics. Empiric use of antibiotics in sick animals was common, and some participants used antibiotics for parasitic diseases. Less than a fifth of participants reported ever using human antibiotics, and use of antibiotics for growth promotion was uncommon.

Conclusion

There is a need to develop Timor-Leste specific treatment guidelines, strengthen veterinary diagnostic support, improve antibiotic procurement, and develop training programs to address knowledge gaps and poor practices found in this study.
Porcine reproductive and respiratory syndrome prevalence and processing fluids use for diagnosis in United States breeding herds
Authors: Kikuti Mariana , Vilalta Carles , Sanhueza Juan , Melini Claudio Marcello , Corzo Cesar A.
Published: November 2022

Introduction

Processing fluids have been recently adopted by the U.S. swine industry as a breeding herd PRRS monitoring tool due to their increased representativeness of animals within the herd. Here, we use the Morrison Swine Health Monitoring Project (MSHMP) database, representative of ~50% of the U.S. swine breeding herd, to describe processing fluids submissions for PRRS diagnosis and their relation to PRRS prevalence and time to stability over time between 2009 and 2020.

Methods

An ecological time series Poisson regression modeling the number of status 1 farms and weekly percentage of processing fluids submissions for PRRS diagnosis was done. Time to stability was calculated for sites that detected a PRRS outbreak within the study period and modeled through a proportional hazards mixed effect survival model using production system as a random-effect factor and epiweek as a panel variable.

Results

Processing fluids diagnosis submissions increased starting in 2017. The difference between each year's highest and lowest weekly prevalence averaged 10.9% between 2009 and 2017, whereas it averaged 5.0% in 2018–2020 period. Each year's lowest weekly prevalence ranged from 11.3 to 19.5% in 2009–2017 and from 22.4 to 29.2% in 2018–2020. We also detected an increasing proportion of breeding sites that did not reach stability within 1 year of reporting an outbreak (chi-square for trend p < 0.0001). The total time to stability was not associated with the region of the country in which the site was located, the site’s air filtration status, its PRRS status before the outbreak, or the different statuses a site achieved to be classified as stable, when accounting for the production system in the multivariate model. However, a higher proportion of system-wide processing fluids use was associated with increased time to stability.

Discussion

Altogether, the temporal concurrence of processing fluids used for PRRS virus monitoring suggests that the adoption of this sampling strategy may help explain the changes observed in PRRS status 1 prevalence since 2018, although further studies are still needed.
Classification of Skin Lesions Using Weighted Majority Voting Ensemble Deep Learning
Authors: Okuboyejo Damilola A. , Olugbara Oludayo O.
Published: November 2022

The conventional dermatology practice of performing noninvasive screening tests to detect skin diseases is a source of escapable diagnostic inaccuracies. Literature suggests that automated diagnosis is essential for improving diagnostic accuracies in medical fields such as dermatology, mammography, and colonography. Classification is an essential component of an assisted automation process that is rapidly gaining attention in the discipline of artificial intelligence for successful diagnosis, treatment, and recovery of patients. However, classifying skin lesions into multiple classes is challenging for most machine learning algorithms, especially for extremely imbalanced training datasets. This study proposes a novel ensemble deep learning algorithm based on the residual network with the next dimension and the dual path network with confidence preservation to improve the classification performance of skin lesions. The distributed computing paradigm was applied in the proposed algorithm to speed up the inference process by a factor of 0.25 for a faster classification of skin lesions. The algorithm was experimentally compared with 16 deep learning and 12 ensemble deep learning algorithms to establish its discriminating prowess. The experimental comparison was based on dermoscopic images congregated from the publicly available international skin imaging collaboration databases. We propitiously recorded up to 82.52% average sensitivity, 99.00% average specificity, 98.54% average balanced accuracy, and 92.84% multiclass accuracy without prior segmentation of skin lesions to outstrip numerous state-of-the-art deep learning algorithms investigated.
Lift Augmentation at Subsonic Speeds by Lateral Jets for a Hypersonic Aircraft
Authors: Wang Haifeng , Liu Jianxia , Deng Feng , Li Guoshu , Ding Yunguang , Xia Qiang , Zhang Fan
Published: November 2022

This paper presents a numerical investigation on the lift augmentation at subsonic speeds by using lateral jets for a hypersonic aircraft equipped with a waverider-type lifting body, which consists of three main parts. The jet slots were arranged along the side edges of the lifting body to study the effect of lateral blowing on the lift augmentation at a freestream Mach number of 0.3. The numerical results based on solving the Reynolds-averaged Navier–Stokes equation indicate that a well-designed lateral blowing can produce a significant lift rise. Then, further work was carried out to investigate the effects of jet parameters, including the jet location, the blowing strength and the blowing direction on lift augmentation, and to provide insights into the associated flow physics. It was found that blowing on the middle and rear parts of the lifting body achieves the maximum lift augmentation among the chosen configurations. Additionally, it was confirmed that the lift augmentation increases as the jet momentum increases, and blowing in the direction of θjet = −45°, which means the jet blows slightly towards the lower surface of the lifting body, produces a larger lift rise than other directions. The lift augmentation can be explained by the fact that a well-designed lateral blowing can amplify the effectiveness of the vortices shedding from the side edges of the lifting body, resulting in an increase in the vortex lift.
Heme Oxygenase-1 and Blood Bilirubin Are Gradually Activated by Oral D-Glyceric Acid
Authors: Hirvonen O. Petteri , Lehti Maarit , Kyröläinen Heikki , Kainulainen Heikki
Published: November 2022

It has been shown that small doses of oral D-glyceric acid (DGA) activate mitochondrial metabolism and reduce inflammation among 50–60-year-old healthy volunteers. The present results with the same small doses reveal that after a 4-day DGA regimen, a dose of DGA activated the HO-1 pathway acutely, while enhanced inflammatory status after the 4-day DGA regimen seemed to be able to downregulate the HO-1 pathway in non-acute measurement. Blood bilirubin was strongly upregulated towards the end of the altogether 21-day study period with positive associations towards improved inflammation and reduced blood triglycerides. After the 4-day DGA regimen, hepatic inflow of blood bilirubin with albumin as the carrier was clearly upregulated in the lower-aerobic-capacity persons. At the same time also, blood triglycerides were down, pointing possibly to the activation of liver fatty acid oxidation. The combination of activated aerobic energy metabolism with transient HO-1 pathway activation and the upregulation of blood bilirubin may reduce the risks of chronic diseases, especially in aging. Furthermore, there exist certain diseases with unsatisfactorily-met medical needs, such as fatty and cholestatic liver diseases, and Parkinson’s disease, that can be possibly ameliorated with the whole-body mechanism of the action of the DGA regimen.
Antioxidant Supplementation in Oxidative Stress-Related Diseases: What Have We Learned from Studies on Alpha-Tocopherol?
Authors: Meulmeester Fleur L. , Luo Jiao , Martens Leon G. , Mills Kevin , van Heemst Diana , Noordam Raymond
Published: November 2022

Oxidative stress has been proposed as a key contributor to lifestyle- and age-related diseases. Because free radicals play an important role in various processes such as immune responses and cellular signaling, the body possesses an arsenal of different enzymatic and non-enzymatic antioxidant defense mechanisms. Oxidative stress is, among others, the result of an imbalance between the production of various reactive oxygen species (ROS) and antioxidant defense mechanisms including vitamin E (α-tocopherol) as a non-enzymatic antioxidant. Dietary vitamins, such as vitamin C and E, can also be taken in as supplements. It has been postulated that increasing antioxidant levels through supplementation may delay and/or ameliorate outcomes of lifestyle- and age-related diseases that have been linked to oxidative stress. Although supported by many animal experiments and observational studies, randomized clinical trials in humans have failed to demonstrate any clinical benefit from antioxidant supplementation. Nevertheless, possible explanations for this discrepancy remain underreported. This review aims to provide an overview of recent developments and novel research techniques used to clarify the existing controversy on the benefits of antioxidant supplementation in health and disease, focusing on α-tocopherol as antioxidant. Based on the currently available literature, we propose that examining the difference between antioxidant activity and capacity, by considering the catabolism of antioxidants, will provide crucial knowledge on the preventative and therapeutical use of antioxidant supplementation in oxidative stress-related diseases.
Photoinhibition of Photosystem I Induced by Different Intensities of Fluctuating Light Is Determined by the Kinetics of ∆pH Formation Rather Than Linear Electron Flow
Authors: Shi Qi , Wang Xiao-Qian , Zeng Zhi-Lan , Huang Wei
Published: November 2022

Fluctuating light (FL) can cause the selective photoinhibition of photosystem I (PSI) in angiosperms. In nature, leaves usually experience FL conditions with the same low light and different high light intensities, but the effects of different FL conditions on PSI redox state and PSI photoinhibition are not well known. In this study, we found that PSI was highly reduced within the first 10 s after transition from 59 to 1809 μmol photons m−2 s−1 in tomato (Solanum lycopersicum). However, such transient PSI over-reduction was not observed by transitioning from 59 to 501 or 923 μmol photons m−2 s−1. Consequently, FL (59-1809) induced a significantly stronger PSI photoinhibition than FL (59-501) and FL (59-923). Compared with the proton gradient (∆pH) level after transition to high light for 60 s, tomato leaves almost formed a sufficient ∆pH after light transition for 10 s in FL (59-501) but did not in FL (59-923) or FL (59-1809). The difference in ∆pH between 10 s and 60 s was tightly correlated to the extent of PSI over-reduction and PSI photoinhibition induced by FL. Furthermore, the difference in PSI photoinhibition between (59-923) and FL (59-1809) was accompanied by the same level of linear electron flow. Therefore, PSI photoinhibition induced by different intensities of FL is more related to the kinetics of ∆pH formation rather than linear electron flow.
Research Trends on the Dispersibility of Carbon Nanotube Suspension with Surfactants in Their Application as Electrodes of Batteries: A Mini-Review
Authors: Yoon Hyungsub , Kim Haeji , Matteini Paolo , Hwang Byungil
Published: November 2022

In the battery field, carbon nanotubes (CNTs) attract much attention due to their potential as a supporting conducting material for anodes or cathodes. The performance of cathodes or anodes can be optimized by introducing densely packed CNTs, which can be achieved with high dispersibility. The efficiency of CNT usage can be maximized by enhancing their dispersibility. An effective technique to this end is to incorporate surfactants on the surface of CNTs. The surfactant produces a surface charge that can increase the zeta potential of CNTs, thereby preventing their agglomeration. Additionally, surfactants having long chains of tail groups can increase the steric hindrance, which also enhances the dispersibility. Notably, the dispersibility of CNTs depends on the type of surfactant. Therefore, the results of dispersibility studies of CNTs involving different surfactants must be comprehensively reviewed to enhance the understanding of the effects of different surfactants on dispersibility. Consequently, this paper discusses the effect of different types of surfactants on the dispersibility of CNTs and presents several perspectives for future research on dispersibility enhancement.
Human–Robot Collaboration and Lean Waste Elimination: Conceptual Analogies and Practical Synergies in Industrialized Construction
Authors: Marinelli Marina
Published: November 2022

The presence of robots in industrial environments is a well-established reality in Industry 4.0 and an absolute necessity in Industry 5.0, with human–robot collaboration (HRC) at the paradigm’s core. Concurrently, lean production remains one of the most influential production paradigms, which strives to eliminate Muda (non-value adding activities), Mura (unevenness), and Muri (people overburdening). However, what conceptual analogies and practical synergies are there between the lean production paradigm and HRC, and how do other Industry 4.0 technologies support this interaction? This research aims to answer this question in the context of industrialized construction, an ideal implementation field for both those approaches. The constructive research methodology is used to showcase, through evidence from the literature, that HRC aimed at the improvement of ergonomics, safety and efficiency has a positive contribution towards the elimination of all the lean wastes, while technologies like AR, VR, wearables, sensors, cloud computing, machine-learning techniques and simulation are crucially important for the intuitiveness of the collaboration between the human and the robotic partner. This is, to the author’s best knowledge, the first attempt to systematically record the commonalities between Lean and HRC, thus enhancing the very limited construction literature related to HRC.
The Prognostic Value of Plasma Programmed Death Protein-1 (PD-1) and Programmed Death-Ligand 1 (PD-L1) in Patients with Gastrointestinal Stromal Tumor
Authors: Brinch Charlotte Margareta , Hogdall Estrid , Junker Niels , Moeller Holger Jon , Sandfeld-Paulsen Birgitte , de Heer Pieter , Penninga Luit , Rossen Philip Blach , Krarup-Hansen Anders , Aggerholm-Pedersen Ninna
Published: November 2022

Background: This study investigates the prognostic value of plasma Programmed Death Protein-1 (PD-1) and Programmed Death-Ligand 1 (PD-L1) concentrations in patients with Gastrointestinal Stromal Tumor (GIST). Methods: Patients with GIST were included (n = 157) from the two Danish sarcoma centers, independent of disease- and treatment status. The patients were divided into three subgroups; 1: patients with localized disease who underwent radical surgery; 2: patients with local, locally advanced, or metastatic disease; and 3: patients without measurable disease who had undergone radical surgery. Sensitive electrochemiluminescence immune-assays were used to determine PD-1 and PD-L1 concentration in plasma samples. The primary endpoint was the PFS. Results: No patients progressed in group 1 (n = 15), 34 progressed in group 2 (n = 122), and three progressed in group 3 (n = 20). Significantly higher plasma concentrations of PD-1 (p = 0.0023) and PD-L1 (0.012) were found in patients in group 2 compared to PD-1/PD-L1 levels in postoperative plasma samples from patient group 1. Patients with active GIST having a plasma concentration of PD-L1 above the cutoff (225 pg/mL) had a significantly poorer prognosis compared to patients with plasma PD-L1 concentration below the cutoff. Conclusions: Plasma PD-L1 shows potential as a prognostic biomarker in patients with GIST and should be further evaluated.
Immunomodulatory Agents for Multiple Myeloma
Authors: Minařík Jiří , Ševčíková Sabina
Published: November 2022

The treatment of multiple myeloma (MM) has undergone a significant paradigm shift in the last 20 years, from conventional chemotherapy to more tumor-specific treatments, based on the interference with pathogenesis of the malignant clone as well as the bone microenvironment [...]
The Analysis of Trends in Survival for Patients with Melanoma Brain Metastases with Introduction of Novel Therapeutic Options before the Era of Combined Immunotherapy—Multicenter Italian–Polish Report
Authors: Placzke Joanna , Teterycz Paweł , Quaglino Pietro , Cybulska-Stopa Bozena , Tucci Marco , Rubatto Marco , Skora Tomasz , Interno Valeria , Rosinska Magdalena , Borkowska Aneta , Szumera-Cieckiewicz Anna , Mandala Mario , Rutkowski Piotr
Published: November 2022

Stage IV melanoma patients develop melanoma brain metastases (MBM) in 50% of cases. Their prognosis is improving, and its understanding outside the context of clinical trials is relevant. We have retrospectively analyzed the clinical data, course of treatment, and outcomes of 531 subsequent stage IV melanoma patients with BM treated in five reference Italian and Polish melanoma centers between 2014 and 2021. Patients with MBM after 2017 had a better prognosis, with a significantly improved median of overall survival (OS) after 2017 in the worst mol-GPA prognostic groups (mol-GPA ≤ 2): a median OS >6 months and HR 0.76 vs. those treated before 2017 (CI: 0.60–0.97, p = 0.027). In our prognostic model, mol-GPA was highly predictive for survival, and symptoms without steroid use did not have prognostic significance. Local therapy significantly improved survival regardless of the year of diagnosis (treated before or after 2017), with median survival >12 months. Systemic therapy improved outcomes when it was combined with local therapy. Local surgery was associated with improved OS regardless of the timing related to treatment start (i.e., before or after 30 days from MBM diagnosis). Local and systemic treatment significantly prolong survival for the poorest mol-GPA prognosis. Use of modern treatment modalities is justified in all mol-GPA prognostic groups.
Implementing the Point Spread Function Deconvolution for Better Molecular Characterization of Newly Diagnosed Gliomas: A Dynamic 18F-FDOPA PET Radiomics Study
Authors: Ahrari Shamimeh , Zaragori Timothée , Bros Marie , Oster Julien , Imbert Laetitia , Verger Antoine
Published: November 2022

Purpose: This study aims to investigate the effects of applying the point spread function deconvolution (PSFd) to the radiomics analysis of dynamic L-3,4-dihydroxy-6-[18F]-fluoro-phenyl-alanine (18F-FDOPA) positron emission tomography (PET) images, to non-invasively identify isocitrate dehydrogenase (IDH) mutated and/or 1p/19q codeleted gliomas. Methods: Fifty-seven newly diagnosed glioma patients underwent dynamic 18F-FDOPA imaging on the same digital PET system. All images were reconstructed with and without PSFd. An L1-penalized (Lasso) logistic regression model, with 5-fold cross-validation and 20 repetitions, was trained with radiomics features extracted from the static tumor-to-background-ratio (TBR) and dynamic time-to-peak (TTP) parametric images, as well as a combination of both. Feature importance was assessed using Shapley additive explanation values. Results: The PSFd significantly modified 95% of TBR, but only 79% of TTP radiomics features. Applying the PSFd significantly improved the ability to identify IDH-mutated and/or 1p/19q codeleted gliomas, compared to PET images not processed with PSFd, with respective areas under the curve of 0.83 versus 0.79 and 0.75 versus 0.68 for a combination of static and dynamic radiomics features (p < 0.001). Without the PSFd, four and eight radiomics features contributed to 50% of the model for detecting IDH-mutated and/or 1p/19q codeleted gliomas, respectively. Application of the PSFd reduced this to three and seven contributive radiomics features. Conclusion: Application of the PSFd to dynamic 18F-FDOPA PET imaging significantly improves the detection of molecular parameters in newly diagnosed gliomas, most notably by modifying TBR radiomics features.
Osseous Union after Jaw Reconstruction with Fibula-Free Flap: Conventional vs. CAD/CAM Patient-Specific Implants
Authors: Knitschke Michael , Yonan Magdalena , Roller Fritz Christian , Pons-Kühnemann Jörn , Attia Sameh , Howaldt Hans-Peter , Streckbein Philipp , Böttger Sebastian
Published: November 2022

This is a monocentric, retrospective study of patients who underwent successful immediate or delayed maxilla or mandible reconstructions with FFF from January 2005 to December 2021. Panoramic radiograph, computed tomography scans, and cone-beam CTs were analyzed concerning the osseous union of the intersegmental junctions between maxillary or mandibular native jaw and fibular bone. The primary parameter was to estimate the status of osseous union according to osteosynthesis type. A total number of 133 patients (PSI: n = 64, non-PSI: n = 69) were included in the present study. The mean age was 56.7 ± 14.0 (Range: 14.7–82.7); the primary diagnosis was in 105 patients a malignant (78.9%) and in 20 patients a benign (15.0%) tumor. Mandible reconstruction was performed on 103 patients (77.4%), and on 30 patients (22.6%), maxilla reconstruction was performed. The radiographic images provided a rate of incomplete osseous union (IOU) of about 90% in both groups in the first 6 months. Imaging between 6 and 12 months reveals an IOU rate in the non-PSI group of 46.3% vs. 52.5% in the PSI group, between 12 and 24 months, an IOU rate of 19.6% vs. 26.1%, between 24 and 36 months 8.9% vs. 21.7%, and after 36 months the IOU rate decreases to 4.2% vs. 18.2%. Multivariate logistic regression shows that only osteosynthesis type (OR = 3.518 [95%-CI = 1.223–10.124], p = 0.02) and adjuvant radiotherapy (OR = 4.804 [95%-CI = 1.602–14.409], p = 0.005) are independent risk factors for incomplete osseous union. Cox regression revealed that the variables plate-system (Hazard ratio, HR = 5.014; 95 %-CI: 1.826–3.769; p = 0.002) and adjuvant radiotherapy (HR = 5.710; 95 %-CI: 2.066–15.787; p < 0.001) are predictors for incomplete osseous union. In our study, the rate of incomplete bony fusion was significantly higher in the PSI group. Jaw-to-fibula apposition zones were significantly more affected than intersegmental zones. In multivariate analysis, a combination of osteosynthesis with PSI and adjuvant radiotherapy could be identified as a risk constellation for incomplete ossification.
VDR Regulates BNP Promoting Neurite Growth and Survival of Cochlear Spiral Ganglion Neurons through cGMP-PKG Signaling Pathway
Authors: Zhang Xinyu , Zhou Ke , Tian Keyong , Zhu Qingwen , Liu Wei , Liu Zhenzhen , An Xiaogang , Tian Chaoyong , Li Yao , Lu Fei , Sun Fei , Zha Dingjun
Published: November 2022

Spiral ganglion neurons (SGNs) are important for hearing, and their peripheral and central processes connect sensory cells of the Corti organ to the central nervous system. The resulting network forms a point-to-point auditory conduction. As a cardiac hormone, brain natriuretic peptide (BNP) binds to natriuretic peptide receptor type A leading to diuresis, vasodilatation, inhibition of renin and aldosterone production, and cardiac and vascular myocyte growth. This study primarily aimed to explore the expression and function of BNP in the rat’s inner ear and elucidate its regulatory mechanism. We determined the expression and function of BNP and found that the vitamin D receptor (VDR) could upregulate the expression of BNP and enhance its function. In SGNs of the rat inner ear, BNP promotes neuron survival and prolongs neurite length through the cGMP-PKG signaling pathway, which could be regulated by VDR and provide a novel approach for neuronal regeneration therapy. To the best of our knowledge, this is the first study to report this potential transcriptional regulatory relationship and will act as a reference for research on neuronal regeneration therapy for SGNs injury.
A Niemann–Pick Disease Type C2 with Severe Pulmonary Involvement and Limited Therapeutic Options: A Case Report
Authors: Al-Shamrani Abdullah , Al-Shamrani Khalid , Mahfoudh Ahmed Bin , Mohamed Ahmed Sarar , Mohamed Sarar
Published: November 2022

Niemann–Pick disease type C (NPC) is an autosomal recessive lipid storage disorder. There are two types, NPC1, which is the predominant form (95%), and the rare NPC2, which represents less than 5% of the reported cases. Niemann–Pick disease type C2 usually presents with respiratory symptoms, cholestasis, neurological impairment, and hepatosplenomegaly. Case report: Here, we report a 3-year-old boy who presented to our hospital with exacerbation of chronic lung disease requiring invasive ventilatory support. He was previously diagnosed with interstitial lung disease. His parents used to instill olive oil in his nose (a few drops in each nostril daily for several months) to treat frequent nasal bleeding. A detailed history revealed prolonged neonatal jaundice for four months, with hepatosplenomegaly. In his second year, generalized hypotonia and delayed psychomotor development were observed. Upon presentation to our institute, chest CT showed evidence of intraparenchymal fat; therefore, lipoid pneumonia and lipid storage disease were suspected. The bronchoalveolar lavage results suggested pulmonary alveolar proteinosis (PAP). Whole-exome sequencing (WES) revealed a class one homozygous pathogenic variant in the NPC2 gene. Our patient faced a range of difficulties, including prolonged mechanical ventilation and diagnostic and therapeutic challenges. Conclusion: Niemann–Pick disease type C2 is a progressive and lethal condition that requires a high index of suspicion to pinpoint the diagnosis. Gene study remains the method of choice to confirm the diagnosis. There are limited choices of therapeutic interventions; therefore, genetic counseling and the prevention of recurrence should be the ultimate goal for affected families.
Plant Diversity along an Urbanization Gradient of a Tropical City
Authors: Alue Balqis Aqila , Salleh Hudin Noraine , Mohamed Fatimah , Mat Said Zahid , Ismail Kamarul
Published: November 2022

This study aimed to investigate the plant diversity, plant traits, and environmental variables along the tropical urbanization gradient in Ipoh, Perak, Malaysia. The study areas comprised 12 sampling plots sized 1 km2 that represented different urbanization intensities. Urbanization intensity was quantified as the percentage of the built-up area within a 1 km2 area. A total of 96 woody plant species belonging to 71 genera and 42 families were found in the study areas. In general, species diversity, richness, and evenness declined significantly as urbanization intensity increased. The number of native species reduced by 67.6% when urbanization intensity increased from wildland to suburban while the non-native species remained stable along the urbanization gradient. Regarding the plant traits, tree height decreased with increasing urbanization intensity, while no significant result was found for specific leaf areas. All environmental factors were significantly associated with urbanization where air temperature and light intensity showed a positive relationship with increasing urbanization intensity while the opposite trend was found for air humidity. This study emphasizes the importance of built-up areas as the predictor of native species in the tropics. The findings of this study may help town planners and policymakers to create more sustainable urban development in the future.
Using Advanced Machine-Learning Algorithms to Estimate the Site Index of Masson Pine Plantations
Authors: Yang Rui , Meng Jinghui
Published: November 2022

The rapid development of non-parametric machine learning methods, such as random forest (RF), extreme gradient boosting (XGBoost), and the light gradient boosting machine (LightGBM), provide new methods to predict the site index (SI). However, few studies used these methods for SI modeling of Masson pine, and there is a lack of comparison of model performances. The purpose of this study was to compare the performance of different modeling approaches and the variability between models with different variables. We used 84 samples from the Guangxi Tropical Forestry Experimental Centre. Five-fold cross-validation was used and linear regression models were established to assess the relationship between the dominant height of the stand and different types of variables. The optimal model was used to predict the SI. The results show that the LightGBM model had the highest accuracy. The root mean square error (RMSE) was 3.4055 m, the relative RMSE (RMSE%) was 20.95, the mean absolute error (MAE) was 2.4189 m, and the coefficient of determination (R2) was 0.5685. The model with climatic and soil chemical variables had an RMSE of 2.7507 m, an RMSE% of 17.18, an MAE of 2.0630 m, and an R2 of 0.6720. The soil physicochemical properties were the most important factors affecting the SI, whereas the ability of the climatic factors to explain the variability in the SI in a given range was relatively low. The results indicate that the LightGBM is an excellent SI estimation method. It has higher efficiency and prediction accuracy than the other methods, and it considers the key factors determining site productivity. Adding climate and soil chemical variables to the model improves the prediction accuracy of the SI and the ability to evaluate site productivity. The proposed Masson pine SI model explains 67.2% of the SI variability. The model is suitable for the scientific management of unevenly aged Masson pine plantations.
COVID-Related Misinformation Migration to BitChute and Odysee
Authors: Papadopoulou Olga , Kartsounidou Evangelia , Papadopoulos Symeon
Published: November 2022

The overwhelming amount of information and misinformation on social media platforms has created a new role that these platforms are inclined to take on, that of the Internet custodian. Mainstream platforms, such as Facebook, Twitter and YouTube, are under tremendous public and political pressure to combat disinformation and remove harmful content. Meanwhile, smaller platforms, such as BitChute and Odysee, have emerged and provide fertile ground for disinformation as a result of their low content-moderation policy. In this study, we analyze the phenomenon of removed content migration from YouTube to BitChute and Odysee. In particular, starting from a list of COVID-related videos removed from YouTube due to violating its misinformation policy, we find that ∼15% (1114 videos) of them migrated to the two low content-moderation platforms under study. This amounts to 4096 videos on BitChute and 1810 on Odysee. We present an analysis of this video dataset, revealing characteristics of misinformation dissemination similar to those on YouTube and other mainstream social media platforms. The BitChute–Odysee COVID-related dataset is publicly available for research purposes on misinformation analysis.