bluuebunny/crossref_metadata_2025 huggingface dataset:
Convolutional Neural Network Classifies Pathological Voice Change in Laryngeal Cancer with High Accuracy
Authors: Kim HyunBum , Jeon Juhyeong , Han Yeon Jae , Joo YoungHoon , Lee Jonghwan , Lee Seungchul , Im Sun
Published: October 2020

Voice changes may be the earliest signs in laryngeal cancer. We investigated whether automated voice signal analysis can be used to distinguish patients with laryngeal cancer from healthy subjects. We extracted features using the software package for speech analysis in phonetics (PRAAT) and calculated the Mel-frequency cepstral coefficients (MFCCs) from voice samples of a vowel sound of /a:/. The proposed method was tested with six algorithms: support vector machine (SVM), extreme gradient boosting (XGBoost), light gradient boosted machine (LGBM), artificial neural network (ANN), one-dimensional convolutional neural network (1D-CNN) and two-dimensional convolutional neural network (2D-CNN). Their performances were evaluated in terms of accuracy, sensitivity, and specificity. The result was compared with human performance. A total of four volunteers, two of whom were trained laryngologists, rated the same files. The 1D-CNN showed the highest accuracy of 85% and sensitivity and sensitivity and specificity levels of 78% and 93%. The two laryngologists achieved accuracy of 69.9% but sensitivity levels of 44%. Automated analysis of voice signals could differentiate subjects with laryngeal cancer from those of healthy subjects with higher diagnostic properties than those performed by the four volunteers.
Health-Related Quality of Life in Patients with Chronic Myeloid Leukemia Treated with First- Versus Second-Generation Tyrosine Kinase Inhibitors
Authors: Shacham Abulafia Adi , Shemesh Sivan , Rosenmann Lena , Berger Tamar , Leader Avi , Sharf Giora , Raanani Pia , Rozovski Uri
Published: October 2020

The life expectancy of patients with chronic myeloid leukemia (CML) approaches that of the age-matched population and quality of life (QOL) issues are becoming increasingly important. To describe patients’ characteristics and assess QOL, we delivered a 30-item core questionnaire, a 24-item CML-specific questionnaire, both from the European Organization for Research and Treatment of Cancer (EORTC), and additional health-related items to 350 patients. Among 193 patients who completed the questionnaires, 139 received either imatinib (n = 70, 33%), dasatinib (n = 45, 23%) or nilotinib (n = 24, 12%). Patients’ median age was 58 (range: 23 to 89) years and 86 (63%) were males. Stratifying patients by treatment, we recognized two distinct populations. In comparison to patients on dasatinib and nilotinib, patients on imatinib were two decades older, had a longer duration of disease and current treatment, experienced fewer limitations on daily activities (p = 0.02), less fatigue (p = 0.001), lower degree of impaired body image (p = 0.022) and less painful episodes (p = 0.014). Similarly, they had better emotional functioning, were less worried, stressed, depressed or nervous (p = 0.01) and were more satisfied with their treatment (p = 0.018). Not only does age associate with current treatments, but it also predicts how patients perceive QOL. Young patients express impaired QOL compared with elderly patients.
On the Reliability of Examining Dual-Tasking Abilities Using a Novel E-Health Device—A Proof of Concept Study in Multiple Sclerosis
Authors: Böttrich Niels , Mückschel Moritz , Dillenseger Anja , Lange Christoph , Kern Raimar , Ziemssen Tjalf , Beste Christian
Published: October 2020

The assessment of neuropsychological functions and especially dual-tasking abilities is considered to be increasingly relevant in the assessment of neurological disease, and Multiple Sclerosis (MS) in particular. However, the assessment of dual-tasking abilities is hindered by specific software requirements and extensive testing times. We designed a novel e-health (progressive web application-based) device for the assessment of dual-tasking abilities usable in “bedside” and outpatient clinic settings and examined its reliability in a sample of N = 184 MS patients in an outpatient setting. Moreover, we examined the relevance of dual-tasking assessment using this device with respect to clinically relevant parameters in MS. We show that a meaningful assessment of dual-tasking is possible within 6 min and that the behavioral readouts overall show good reliability depending on dual-tasking difficulty. We show that dual-tasking readouts were correlated with clinically relevant parameters (e.g., EDSS, disease duration, processing speed) and were not affected by fatigue levels. We consider the tested dual-tasking assessment device suitable for routine clinical neuropsychological assessments of dual-tasking abilities. Future studies may further evaluate this test regarding its suitability in the long-term follow up assessments and to assess dual-tasking abilities in other neurological and psychiatric disorders.
Numerical Simulation of Scour Depth and Scour Patterns in Front of Vertical-Wall Breakwaters Using OpenFOAM
Authors: Karagiannis Nikolaos , Karambas Theophanis , Koutitas Christopher
Published: October 2020

An advanced coupled numerical model was developed and implemented in the present work, describing the scour patterns and predicting the scour depth in front of vertical-wall breakwaters. It consists of two independent models, a hydrodynamic Computational Fluid Dynamics (CFD) Reynolds Averaged Navier–Stokes (RANS) model developed on the OpenFOAM (version 2.4.0) toolbox (CFD), describing the wave propagation and the associated hydrodynamic field, and a morphodynamic one (sediment transport model), which was developed in FORTRAN by the authors and yields the updated seabed morphology. The method used here is iterative. The hydrodynamic model is applied for any given initial seabed geometry and wave conditions, resulting in the hydrodynamic field of the flow, which is used as input by the second sediment transport model for the seabed morphology evolution. This process is repeated until the equilibrium profile is achieved. Model results are compared satisfactorily with experimental data for both scour patterns and prediction of scour depth.
Self-Organized Anodic Oxides on Titanium Alloys Prepared from Glycol- and Glycerol-Based Electrolytes
Authors: Michalska-Domańska Marta , Łazińska Magdalena , Łukasiewicz Justyna , Mol Johannes M. C. , Durejko Tomasz
Published: October 2020

The anodization of commercially pure Ti alloy (99.5 wt %) and two biomedical titanium alloys, Ti6Al7Nb and Ti6Al4V, was performed, and the resulting anodic oxides were studied. The biomedical alloys were made by Laser Engineered Net Shaping. The glycol-based and glycerol-based electrolytes with 0.3 M ammonium fluoride and 2 wt % of deionized water content were tested. It was found that electrolyte type as well as the chemical composition of the base substrate affected the final morphology and chemical composition of the anodic oxide formed. A higher current density, ionic mobility, and oxide growth rate were obtained in glycol-based electrolyte as compared to those obtained in glycerol-based electrolyte for all tested alloys. A self-organized nanotubular and nanoporous morphology of the anodic oxide in both types of electrolyte was obtained. In each electrolyte, the alloy susceptibility to oxidation increased in the following order: Ti6Al4V < Ti 99.5% < Ti6Al7Nb, which can be correlated to the oxidation susceptibility of the base titanium alloy. It was observed that the more impurities/alloying elements in the substrate, the lower the pore diameters of anodic oxide. There was a higher observed incorporation of electrolyte species into the anodic oxide matrix in the glycerol-based electrolyte compared with that in glycol-based electrolyte.
Sequential Improvement from Cosolvents Ink Formulation to Vacuum Annealing for Ink-Jet Printed Quantum-Dot Light-Emitting Diodes
Authors: Han Young Joon , Kim Do Yeob , An Kunsik , Kang Kyung-Tae , Ju Byeong-Kwon , Cho Kwan Hyun
Published: October 2020

Optimization of ink-jet printing conditions of quantum-dot (QD) ink by cosolvent process and improvement of quantum-dot light-emitting diodes (QLEDs) characteristics assisted by vacuum annealing were analyzed in this research. A cosolvent process of hexane and ortho-dichlorobenzene (oDCB) was optimized at the ratio of 1:2, and ink-jetting properties were analyzed using the Ohnesorge number based on the parameters of viscosity and surface tension. However, we found that these cosolvents systems cause an increase in the boiling point and a decrease in the vapor pressure, which influence the annealing characteristics of the QD emission layer (EML). Therefore, we investigated QLEDs’ performance depending on the annealing condition for ink-jet printed QD EML prepared using cosolvents systems of hexane and oDCB. We enhanced the quality of QD EML and device performance of QLEDs by a vacuum annealing process, which was used to prevent exposure to moisture and oxygen and to promote effective evaporation of solvent in QD EML. As a result, the characteristics of QLEDs formed using ink-jet printed QD EML annealed under vacuum environment increased luminescence (L), current efficiency (CE), external quantum efficiency (EQE), and lifetime (LT50) by 30.51%, 33.7%, 21.70%, and 181.97%, respectively, compared to QLEDs annealed under air environment.
Ultra-Wideband RCS Reduction Based on Non-Planar Coding Diffusive Metasurface
Authors: Wu Guozhang , Yu Wenqi , Lin Tao , Deng Yangyang , Liu Jianguo
Published: October 2020

A novel non-planar coding metasurface optimized by discrete particle swarm algorithm (DPSO) is proposed in terms of the property of wideband radar cross-section (RCS) and diffuse scattering. The design consists of two unit cells, “0” and “1”, which have a 180° ± 37° phase difference for phase interference cancellation. The 10 dB monostatic RCS reduction frequency range of the metasurface is from 6.4 to 29.6 GHz, and its bandwidth ratio is 4.62:1, under normal incidence of the two polarizations. Compared to the planar surface, the non-planar surface has a greater bandwidth with respect to the monostatic and bistatic RCS reduction. The results declare its properties of ultra-wideband, angle insensitivity, and polarization insensitivity. Finally, the theoretical analysis, simulation, and experimental results match perfectly, indicating that the metasurface can be used in the RCS reduction or other microwave applications with wider RCS reduction and diffuse scattering.
Transitivity in Fuzzy Hyperspaces
Authors: Jardón Daniel , Sánchez Iván , Sanchis Manuel
Published: October 2020

Given a metric space (X,d), we deal with a classical problem in the theory of hyperspaces: how some important dynamical properties (namely, weakly mixing, transitivity and point-transitivity) between a discrete dynamical system f:(X,d)→(X,d) and its natural extension to the hyperspace are related. In this context, we consider the Zadeh’s extension f^ of f to F(X), the family of all normal fuzzy sets on X, i.e., the hyperspace F(X) of all upper semicontinuous fuzzy sets on X with compact supports and non-empty levels and we endow F(X) with different metrics: the supremum metric d∞, the Skorokhod metric d0, the sendograph metric dS and the endograph metric dE. Among other things, the following results are presented: (1) If (X,d) is a metric space, then the following conditions are equivalent: (a) (X,f) is weakly mixing, (b) ((F(X),d∞),f^) is transitive, (c) ((F(X),d0),f^) is transitive and (d) ((F(X),dS)),f^) is transitive, (2) if f:(X,d)→(X,d) is a continuous function, then the following hold: (a) if ((F(X),dS),f^) is transitive, then ((F(X),dE),f^) is transitive, (b) if ((F(X),dS),f^) is transitive, then (X,f) is transitive; and (3) if (X,d) be a complete metric space, then the following conditions are equivalent: (a) (X×X,f×f) is point-transitive and (b) ((F(X),d0) is point-transitive.
Diphlorethohydroxycarmalol (DPHC) Isolated from the Brown Alga Ishige okamurae Acts on Inflammatory Myopathy as an Inhibitory Agent of TNF-α
Authors: Kim Seo-Young , Ahn Ginnae , Kim Hyun-Soo , Je Jun-Geon , Kim Kil-Nam , Jeon You-Jin
Published: October 2020

Inflammation affects various organs of the human body, including skeletal muscle. Phlorotannins are natural biologically active substances found in marine brown algae and exhibit anti-inflammatory activities. In this study, we focused on the effects of phlorotannins on anti-inflammatory activity and skeletal muscle cell proliferation activity to identify the protective effects on the inflammatory myopathy. First, the five species of marine brown algal extracts dramatically inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 cells without toxicity at all the concentrations tested. Moreover, the extracts collected from Ishige okamurae (I. okamurae) significantly increased cell proliferation of C2C12 myoblasts compared to the non-treated cells with non-toxicity. In addition, as a result of finding a potential tumor necrosis factor (TNF)-α inhibitor that regulates the signaling pathway of muscle degradation in I. okamurae-derived natural bioactive compounds, Diphlorethohydroxycarmalol (DPHC) is favorably docked to the TNF-α with the lowest binding energy and docking interaction energy value. Moreover, DPHC down-regulated the mRNA expression level of pro-inflammatory cytokines and suppressed the muscle RING-finger protein (MuRF)-1 and Muscle Atrophy F-box (MAFbx)/Atrgoin-1, which are the key protein muscle atrophy via nuclear factor-κB (NF-κB), and mitogen-activated protein kinase (MAPKs) signaling pathways in TNF-α-stimulated C2C12 myotubes. Therefore, it is expected that DPHC isolated from IO would be developed as a TNF-α inhibitor against inflammatory myopathy.
Characterization of a Coproduct from the Sea Cucumber Cucumaria frondosa and Its Effects on Visceral Adipocyte Size in Male Wistar Rats
Authors: Ramalho Alan , Leblanc Nadine , Fortin Marie-Gil , Marette André , Tchernof André , Jacques Hélène
Published: October 2020

Sea cucumbers have been shown to have potential health benefits and are a rich source of several bioactive compounds, particularly triterpenoid saponins. However, most studies concentrate on the body wall, and little is known about the health effects of the coproducts. The objectives of this study were to determine the nutritional composition of a coproduct from the sea cucumber Cucumaria frondosa and the effects of the dietary consumption of this coproduct on cardiometabolic health in rats. Chemical, biochemical, and nutritional analyses were performed to characterize this coproduct. Forty (40) male Wistar rats were then equally divided into four groups and fed a purified control diet or a diet enriched with 0.5%, 1.5%, or 2.5% (by protein) of coproduct. After 28 days of feeding, the rats were sacrificed. Body and tissue weight, body composition, epididymal adipocyte diameter, plasma and hepatic lipids, glycemia, and insulinemia were measured at the end of the 28-day experiment. Analysis of the coproduct revealed high levels of protein, omega-3 fatty acids, minerals, and saponins. The 1.5% group had significantly smaller epididymal adipocytes vs. the control. We conclude that dietary administration of this sea cucumber coproduct at 1.5% doses decreases visceral adiposity, potentially decreasing the risk of cardiometabolic dysfunction. The coproduct’s saponin content may contribute to the observed effects, but the impact of other components cannot be ruled out.
Two Lithuanian Cases of Classical Galactosemia with a Literature Review: A Novel GALT Gene Mutation Identified
Authors: Rokaitė Rūta , Traberg Rasa , Dženkaitis Mindaugas , Kučinskienė Rūta , Labanauskas Liutauras
Published: October 2020

Galactosemia is a rare autosomal recessive genetic disorder that causes impaired metabolism of the carbohydrate galactose. This leads to severe liver and kidney insufficiency, central nervous system damage and long-term complications in newborns. We present two clinical cases of classical galactosemia diagnosed at the Lithuanian University of Health Sciences (LUHS) Kaunas Clinics hospital and we compare these cases in terms of clinical symptoms and genetic variation in the GALT gene. The main clinical symptoms were jaundice and hepatomegaly, significant weight loss, and lethargy. The clinical presentation of the disease in Patient 1 was more severe than that in Patient 2 due to liver failure and E. coli-induced sepsis. A novel, likely pathogenic GALT variant NM_000155.4:c.305T>C (p.Leu102Pro) was identified and we believe it could be responsible for a more severe course of the disease, although further study is needed to confirm this. It is very important to suspect and diagnose galactosemia as early in its course as possible, and introduce lactose-free formula into the patient’s diet. Wide-scale newborn screening and genetic testing are particularly crucial for the early detection of the disease.
Effect of Heat Treatment on Microstructure and Mechanical Properties of New Cold-Rolled Automotive Steels
Authors: Huang Fei , Chen Jian , Ge Zhangqi , Li Junliang , Wang Yongqiang
Published: October 2020

The effect of austenitizing temperature and aging treatment on the microstructure and mechanical properties of two new cold-rolled automotive steel plates (20Mn2Cr and 20Mn2CrNb) was investigated by using isothermal heat treatment, optical microscope, scanning electron microscope, microhardness tester, and tensile testing machine. The results show that as the austenitizing temperature increased, the original austenite grain sizes of both steels increased. The original austenite grain size of 20Mn2CrNb was smaller than that of 20Mn2Cr. The microhardness of 20Mn2CrNb gradually decreased with increasing aging temperature, while the hardness of 20Mn2Cr varied irregularly. The mechanical properties of 20Mn2Cr were better than those of 20Mn2CrNb under the same heat-treatment process. The effect of heat treatment on microstructure and mechanical properties was related to the martensite content, dislocation density, and precipitation of second-phase particles.
Finite Difference Modeling of the Interstand Evolutions of Profile and Residual Stress during Hot Strip Rolling
Authors: Yao Chihuan , He Anrui , Shao Jian , Zhao Jianwei , Zhou Guanyu , Li Hui , Qiang Yi
Published: October 2020

Elastic recovery and viscoplastic stress relaxation occur in the interstand of hot rolling, impacting the evolutions of strip profile and residual stress, which are major concerns for obtaining high-quality flat products. A better understanding of the evolutionary mechanisms would help develop shape control strategies. Therefore, a quasi-3D steady-state elasto-viscoplastic rolling model is developed based on the finite difference method. Predictions of spread, profile, and residual stress are validated through comparisons with a two-stand finite element model. The new model is also complemented with a roll stack model and with a viscoplastic constitutive model calibrated by hot compression tests to simulate a seven-stand hot rolling industrial experiment with low carbon steel. Comparisons between the predicted and measured profiles show a satisfactory accuracy. The simulation costs approximately a minute of CPU time, enabling the new model to run massive parametric campaigns for process optimization. It is found that during the interstand elastic recovery, the transverse compressive stress releases and the strip velocity tends to be uniform, revealing residual stress after a significant change of stress pattern. The stress relaxation mainly occurs at the edge near the roll bite and therefore increases the edge drop of the profile; it also decreases the center crown by changing the distribution of the rolling pressure in the roll bite.
The Role of Metabolic Enzymes in the Regulation of Inflammation
Authors: Godfrey Wesley H. , Kornberg Michael D.
Published: October 2020

Immune cells undergo dramatic metabolic reprogramming in response to external stimuli. These metabolic pathways, long considered as simple housekeeping functions, are increasingly understood to critically regulate the immune response, determining the activation, differentiation, and downstream effector functions of both lymphoid and myeloid cells. Within the complex metabolic networks associated with immune activation, several enzymes play key roles in regulating inflammation and represent potential therapeutic targets in human disease. In some cases, these enzymes control flux through pathways required to meet specific energetic or metabolic demands of the immune response. In other cases, key enzymes control the concentrations of immunoactive metabolites with direct roles in signaling. Finally, and perhaps most interestingly, several metabolic enzymes have evolved moonlighting functions, with roles in the immune response that are entirely independent of their conventional enzyme activities. Here, we review key metabolic enzymes that critically regulate inflammation, highlighting mechanistic insights and opportunities for clinical intervention.
Nitric Oxide in Macrophage Immunometabolism: Hiding in Plain Sight
Authors: Palmieri Erika M. , McGinity Christopher , Wink David A. , McVicar Daniel W.
Published: October 2020

Nitric Oxide (NO) is a soluble endogenous gas with various biological functions like signaling, and working as an effector molecule or metabolic regulator. In response to inflammatory signals, immune myeloid cells, like macrophages, increase production of cytokines and NO, which is important for pathogen killing. Under these proinflammatory circumstances, called “M1”, macrophages undergo a series of metabolic changes including rewiring of their tricarboxylic acid (TCA) cycle. Here, we review findings indicating that NO, through its interaction with heme and non-heme metal containing proteins, together with components of the electron transport chain, functions not only as a regulator of cell respiration, but also a modulator of intracellular cell metabolism. Moreover, diverse effects of NO and NO-derived reactive nitrogen species (RNS) involve precise interactions with different targets depending on concentration, temporal, and spatial restrictions. Although the role of NO in macrophage reprogramming has been in evidence for some time, current models have largely minimized its importance. It has, therefore, been hiding in plain sight. A review of the chemical properties of NO, past biochemical studies, and recent publications, necessitates that mechanisms of macrophage TCA reprogramming during stimulation must be re-imagined and re-interpreted as mechanistic results of NO exposure. The revised model of metabolic rewiring we describe here incorporates many early findings regarding NO biochemistry and brings NO out of hiding and to the forefront of macrophages immunometabolism.
Geochemical Characterization of Zircon in Fyfe Hills of the Napier Complex, East Antarctica
Authors: Takehara Mami , Horie Kenji , Hokada Tomokazu
Published: October 2020

Ultra-high temperature (UHT) metamorphism plays an essential role in the development and stabilization of continents through accretionary and collisional orogenesis. The Napier Complex, East Antarctica, preserves UHT metamorphism, and the timing is still debated. U–Pb zircon geochronology integrated with rare earth element (REE) and oxygen isotope was applied to a garnet-bearing quartzo-feldspathic gneiss to confirm the timing of UHT metamorphism in Fyfe Hills in the western part of the Napier Complex. The zircons are analyzed using a sensitive high-resolution ion microprobe (SHRIMP). The cathodoluminescence observation and U–Pb ages allowed us to classify the analytical domains into three types: inherited domains (Group I), metamorphic domains (Group II), and U–Pb system disturbed domains (Group III). The REE patterns of Group II are characterized by a weak fractionation between the middle REE and heavy REE, which reinforces the above classification. The 207Pb*/206Pb* ages of Group II have an age peak at 2501 Ma, therefore, the gneiss experienced high temperature metamorphism at 2501 Ma. δ18O of zircons are homogeneous among the three groups (5.53 ± 0.11‰, 5.51 ± 0.14‰, and 5.53 ± 0.23‰), which suggests re-equilibration of oxygen isotopes after metamorphism at ca. 2501Ma under dry UHT conditions.
Oxidizing Roasting Behavior and Leaching Performance for the Recovery of Spent LiFePO4 Batteries
Authors: Jie Yafei , Yang Shenghai , Li Yun , Zhao Duoqiang , Lai Yanqing , Chen Yongming
Published: October 2020

In this study, the effects of oxidizing roasting process on the liberation of cathode materials from Al foil under different conditions were investigated systematically. The mineralogical characteristics of the cathode materials before and after thermal treatment were extensively characterized using scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) as well as Fourier transform infrared (FT-IR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The results indicated that the increase in roasting temperature, oxygen concentration, and air flow rate enhanced the liberation of cathode materials. The cathode materials were gradually oxidized to Li3Fe2(PO4)3 and Fe2O3. Further, the carbon and fluorine content in the cathode materials decreased slowly during the thermal treatment, while the Al content increased. When the roasting temperature exceeded the melting point of Al, the Al foils were ablated and the cathode materials adhered to the Al foils again, resulting in difficulty in separation. The cathode materials leaching performance test results demonstrated that the oxidation of cathode materials had a negative effect on the leaching of Fe in sulfuric acid leaching system.
Iron Isotopes Constrain the Metal Sources of Skarn Deposits: A Case Study from the Han-Xing Fe Deposit, China
Authors: Zhu Bin , Zhang Hongfu , Santosh M. , Su Benxun , Zhang Pengfei , Han Chunming , He Yongsheng
Published: October 2020

Magmatic fluids and leaching of rocks are regarded as the two sources of magmatic hydrothermal deposits, but their relative contributions to the metals in the deposits are still unclear. In this study, we combine major elements and Fe isotopes in two sets of rocks from the Han-Xing iron skarn deposit in China to constrain the iron sources. The positive correlation between the δ56Fe and ∑Fe2O3/TiO2 of altered diorites (∑Fe2O3 refers to the total iron) demonstrates that heavy Fe isotopes are preferentially leached from diorites during hydrothermal alteration. However, except for the pyrite, all the rocks and minerals formed in the skarn deposit are enriched in the light Fe isotope relative to the fresh/less altered diorites. Therefore, besides the leaching of rocks, the Fe isotopically light magmatic fluid also provides a large quantity of iron for this deposit. Based on the mass balance calculation, we conclude that iron from magmatic fluid is almost 2.6 times as large as that from the leaching of rocks. This is the first study to estimate the relative proportions of iron sources for Fe deposits by using Fe isotopes. Here, we propose that the high δ56Fe of magmatic intrusions combining the positive correlation between their ∑Fe2O3/TiO2 and δ56Fe could be taken as a fingerprint of exsolution or interaction with magmatic fluids, which contributes to the exploration of magmatic hydrothermal ore deposits.
Application of Ionic Liquids for Chemical Demulsification: A Review
Authors: Hassanshahi Nahid , Hu Guangji , Li Jianbing
Published: October 2020

In recent years, ionic liquids have received increasing interests as an effective demulsifier due to their characteristics of non-flammability, thermal stability, recyclability, and low vapor pressure. In this study, emulsion formation and types, chemical demulsification system, the application of ionic liquids as a chemical demulsifier, and key factors affecting their performance were comprehensively reviewed. Future challenges and opportunities of ionic liquids application for chemical demulsification were also discussed. The review indicted that the demulsification performance was affected by the type, molecular weight, and concentration of ionic liquids. Moreover, other factors, including the salinity of aqueous phase, temperature, and oil types, could affect the demulsification process. It can be concluded that ionic liquids can be used as a suitable substitute for commercial demulsifiers, but future efforts should be required to develop non-toxic and less expensive ionic liquids with low viscosity, and the demulsification efficiency could be improved through the application of ionic liquids with other methods such as organic solvents.
Extremophilic Microorganisms for the Treatment of Toxic Pollutants in the Environment
Authors: Jeong Sun-Wook , Choi Yong Jun
Published: October 2020

As concerns about the substantial effect of various hazardous toxic pollutants on the environment and public health are increasing, the development of effective and sustainable treatment methods is urgently needed. In particular, the remediation of toxic components such as radioactive waste, toxic heavy metals, and other harmful substances under extreme conditions is quite difficult due to their restricted accessibility. Thus, novel treatment methods for the removal of toxic pollutants using extremophilic microorganisms that can thrive under extreme conditions have been investigated during the past several decades. In this review, recent trends in bioremediation using extremophilic microorganisms and related approaches to develop them are reviewed, with relevant examples and perspectives.
Chemical Profile and Use of the Peat as an Adsorbent for Extraction of Volatile Compounds from Leaves of Geranium (Pelargonium graveolens L’ Herit)
Authors: Niculau Edenilson dos Santos , Alves Péricles Barreto , Nogueira Paulo Cesar de Lima , Romão Luciane Pimenta Cruz , Cunha Graziele da Costa , Blank Arie Fitzgerald , Silva Anderson de Carvalho
Published: October 2020

Volatile organic compounds (VOCs) from leaves of geranium (Pelargonium graveolens L’ Herit) were extracted by dynamic headspace using Porapak Q (HSD-P) as adsorbent and peat, a novel adsorbent in the extraction of plant volatiles, analyzed by gas chromatography–mass spectrometry (GC/MS) and gas chromatography–flame ionization (GC/FID), and the results were compared with those obtained by hydrodistillation (HD). The yield volatiles changed with the extraction method. HD was more efficient for extracting linalool (11.19%) and citronellyl formate (9.41%). Citronellol (28.06%), geraniol (38.26%) and 6,9-guaiadiene (9.55%) and geranyl tiglate (8.21%) were the major components identified by dynamic headspace using peat (HSD-T), while citronellol (16.88%), geraniol (13.63%), 6,9-guaiadiene (16.98%) and citronellyl formate (6.95%) were identified by dynamic headspace using Porapak Q (HSD-P). Furthermore, this work showed, for the first time, that in natura peat is useful to extract VOCs from leaves of geranium.
Nutrients Supplementation through Organic Manures Influence the Growth of Weeds and Maize Productivity
Authors: Ghosh Dibakar , Brahmachari Koushik , Skalicky Milan , Hossain Akbar , Sarkar Sukamal , Dinda Nirmal Kumar , Das Anupam , Pramanick Biswajit , Moulick Debojyoti , Brestic Marian , Raza Muhammad Ali , Barutcular Celaleddin , Fahad Shah , Saneoka Hirofumi , EL Sabagh Ayman
Published: October 2020

Declining rate of productivity and environmental sustainability is forcing growers to use organic manures as a source of nutrient supplement in maize farming. However, weed is a major constraint to maize production. A field study was carried out over two seasons to evaluate various integrated nutrient and weed management practices in hybrid maize. The treatment combinations comprised of supplementation of inorganic fertilizer (25% nitrogen) through bulky (Farmyard manure and vermicompost) and concentrated (Brassicaceous seed meal (BSM) and neem cake (NC)) organic manures and different mode of weed management practices like chemical (atrazine 1000 g ha−1) and integrated approach (atrazine 1000 g ha−1 followed by mechanical weeding). Repeated supplementation of nitrogen through concentrated organic manures reduced the density and biomass accumulation of most dominant weed species, Anagalis arvensis by releasing allelochemicals into the soil. But organic manures had no significant impact on restricting the growth of bold seeded weeds like Vicia hirsuta and weed propagated through tubers i.e., Cyperus rotundus in maize. By restricting the weed growth and nutrient removal by most dominating weeds, application of BSM enhanced the growth and yield of maize crop. Repeated addition of organic manures (BSM) enhanced the maize grain yield by 19% over sole chemical fertilizer in the second year of study. Application of atrazine as pre-emergence (PRE) herbicide significantly reduced the density of A. arvensis, whereas integration of mechanical weeding following herbicide controlled those weeds which were not usually controlled with the application of atrazine. As a result, atrazine at PRE followed by mechanical weeding produced the highest maize grain yield 6.81 and 7.10 t/ha in the first year and second year of study, respectively.
Attenuation of Nrf2/Keap1/ARE in Alzheimer’s Disease by Plant Secondary Metabolites: A Mechanistic Review
Authors: Fakhri Sajad , Pesce Mirko , Patruno Antonia , Moradi Seyed Zachariah , Iranpanah Amin , Farzaei Mohammad Hosein , Sobarzo-Sánchez Eduardo
Published: October 2020

Alzheimer’s disease (AD) is a progressive neuronal/cognitional dysfunction, leading to disability and death. Despite advances in revealing the pathophysiological mechanisms behind AD, no effective treatment has yet been provided. It urges the need for finding novel multi-target agents in combating the complex dysregulated mechanisms in AD. Amongst the dysregulated pathophysiological pathways in AD, oxidative stress seems to play a critical role in the pathogenesis progression of AD, with a dominant role of nuclear factor erythroid 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein-1 (Keap1)/antioxidant responsive elements (ARE) pathway. In the present study, a comprehensive review was conducted using the existing electronic databases, including PubMed, Medline, Web of Science, and Scopus, as well as related articles in the field. Nrf2/Keap1/ARE has shown to be the upstream orchestrate of oxidative pathways, which also ameliorates various inflammatory and apoptotic pathways. So, developing multi-target agents with higher efficacy and lower side effects could pave the road in the prevention/management of AD. The plant kingdom is now a great source of natural secondary metabolites in targeting Nrf2/Keap1/ARE. Among natural entities, phenolic compounds, alkaloids, terpene/terpenoids, carotenoids, sulfur-compounds, as well as some other miscellaneous plant-derived compounds have shown promising future accordingly. Prevailing evidence has shown that activating Nrf2/ARE and downstream antioxidant enzymes, as well as inhibiting Keap1 could play hopeful roles in overcoming AD. The current review highlights the neuroprotective effects of plant secondary metabolites through targeting Nrf2/Keap1/ARE and downstream interconnected mediators in combating AD.
Engineered Zero-Dimensional Fullerene/Carbon Dots-Polymer Based Nanocomposite Membranes for Wastewater Treatment
Authors: Jani Mona , Arcos-Pareja Jose A. , Ni Ming
Published: October 2020

With the rapid growth of industrialization, diverse pollutants produced as by-products are emitted to the air-water ecosystem, and toxic contamination of water is one of the most hazardous environmental issues. Various forms of carbon have been used for adsorption, electrochemical, and ion-exchange membrane filtration to separation processes for water treatment. The utilization of carbon materials has gained tremendous attention as they have exceptional properties such as chemical, mechanical, thermal, antibacterial activities, along with reinforcement capability and high thermal stability, that helps to maintain the ecological balance. Recently, engineered nano-carbon incorporated with polymer as a composite membrane has been spotlighted as a new and effective mode for water treatment. In particular, the properties of zero-dimensional (0D) carbon forms (fullerenes and carbon dots) have encouraged researchers to explore them in the field of wastewater treatment through membrane technologies as they are biocompatible, which is the ultimate requirement to ensure the safety of drinking water. Thus, the purpose of this review is to highlight and summarize current advances in the field of water purification/treatment using 0D carbon-polymer-based nanocomposite membranes. Particular emphasis is placed on the development of 0D carbon forms embedded into a variety of polymer membranes and their influence on the improved performance of the resulting membranes. Current challenges and opportunities for future research are discussed.
Effects of Modifying Thioflavin T at the N3-Position on Its G4 Binding and Fluorescence Emission
Authors: Kataoka Yuka , Fujita Hiroto , Endoh Tamaki , Sugimoto Naoki , Kuwahara Masayasu
Published: October 2020

We previously synthesized thioflavin T (ThT) with a hydroxyethyl group introduced at the N3-position (ThT-HE), which binds predominantly to the parallel G-quadruplex (G4) structure found in c-Myc and emits strong fluorescence. In this study, to investigate the effects of introduced substituents on G4 binding and fluorescence emission, a ThT derivative in which the hydroxyl group of ThT-HE was replaced with an amino group (ThT-AE) was synthesized for the first time. Furthermore, three other N3-modified ThT derivatives (ThT-OE2, ThT-SP, and ThT-OE11) having different substituent structures were synthesized by the N-acylation of the terminal amino group of ThT-AE, and their G4-binding and emission properties were investigated. The results showed that, although ThT-AE shows binding selectivity depending on the type of G4, its emission intensity is significantly decreased as compared to that of ThT-HE. However, ThT-OE11, which features an 11-unit oxyethylene chain attached to the terminal amino group of ThT-AE, regained about one-half of the emission intensity of ThT-HE while retaining selectivity for G4s. Accordingly, ThT-OE11 may be used as a key intermediate for synthesizing the conjugates of G4 binders and probes.
Small Molecules Targeting Biological Clock; A Novel Prospective for Anti-Cancer Drugs
Authors: Rahman Sadia , Wittine Karlo , Sedić Mirela , Markova-Car Elitza P.
Published: October 2020

The circadian rhythms are an intrinsic timekeeping system that regulates numerous physiological, biochemical, and behavioral processes at intervals of approximately 24 h. By regulating such processes, the circadian rhythm allows organisms to anticipate and adapt to continuously changing environmental conditions. A growing body of evidence shows that disruptions to the circadian rhythm can lead to various disorders, including cancer. Recently, crucial knowledge has arisen regarding the essential features that underlie the overt circadian rhythm and its influence on physiological outputs. This knowledge suggests that specific small molecules can be utilized to control the circadian rhythm. It has been discovered that these small molecules can regulate circadian-clock-related disorders such as metabolic, cardiovascular, inflammatory, as well as cancer. This review examines the potential use of small molecules for developing new drugs, with emphasis placed on recent progress that has been made regarding the identification of small-molecule clock modulators and their potential use in treating cancer.
Modulation of Lipid Metabolism by Trans-Anethole in Hepatocytes
Authors: Song Ahran , Park Yoonjin , Kim Boyong , Lee Seung Gwan
Published: October 2020

Non-alcoholic fatty liver disease is caused by excessive lipid accumulation in hepatocytes. Although trans-anethole (TAO) affects hypoglycemia and has anti-immune activity and anti-obesity effects, its role in non-alcoholic fatty liver disease remains unknown. This study aimed to evaluate the effects of TAO on cellular senescence, lipid metabolism, and reinforcement of microenvironments in HepG2 cells. To analyze the lipid metabolic activity of TAO, PCR analysis, flow-cytometry, and Oil Red O staining were performed, and mitochondrial membrane potential (MMP) and cellular senescence kits were used for assessing the suppression of cellular senescence. At 2000 μg/mL TAO, the cellular viability was approximately 99%, and cell senescence decreased dose-dependently. In the results for MMP, activity increased with concentration. The levels of lipolytic genes, CPT2, ACADS, and HSL, strongly increased over 3 days and the levels of lipogenic genes, ACC1 and GPAT, were downregulated on the first day at 1000 μg/mL TAO. Consequently, it was found that TAO affects the suppression of cellular senescence, activation of lipid metabolism, and reinforcement of the microenvironment in HepG2 cells, and can be added as a useful component to functional foods to prevent fatty liver disease and cellular senescence, as well as increase the immunoactivity of the liver.
Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta
Authors: Sánchez-Pastor Enrique , Trujillo Xóchitl , Ramos-Flores Christian , Ríos-Silva Mónica , Andrade Felipa , Cárdenas Yolitzy , Castro Elena , Urzúa Zorayda , Newton-Sánchez Oscar , Huerta Miguel
Published: October 2020

Previous studies have suggested a role of the endocannabinoid system in metabolic diseases, such as diabetes. We investigated the effect of diabetes on cannabinoid receptor type 1 (CB1) expression and cannabinoid-induced vasorelaxation in rat aorta rings. Aortas from healthy rats and from rats with experimentally induced diabetes were used to compare the vasorelaxant effect of the cannabinoid agonist arachidonylcyclopropylamide (ACPA) and CB1 expression and localization. After 4–8 weeks of diabetes induction, CB1 receptor expression and CB1 phosphorylation were higher in aortic rings, in association with greater vasorelaxation induced by the CB1 agonist ACPA compared to healthy rats. The vasorelaxant effect observed in healthy rats is similar throughout the study. Further studies are needed to elucidate the implications of CB1 receptor overexpression in diabetes and its influence on the progression of the cardiovascular complications of this metabolic disease.
Mimicking the Nucleosomal Context in Peptide-Based Binders of a H3K36me Reader Increases Binding Affinity While Altering the Binding Mode
Authors: Horn Velten , Jongkees Seino A. K. , van Ingen Hugo
Published: October 2020

Targeting of proteins in the histone modification machinery has emerged as a promising new direction to fight disease. The search for compounds that inhibit proteins that readout histone modification has led to several new epigenetic drugs, mostly for proteins involved in recognition of acetylated lysines. However, this approach proved to be a challenging task for methyllysine readers, which typically feature shallow binding pockets. Moreover, reader proteins of trimethyllysine K36 on the histone H3 (H3K36me3) not only bind the methyllysine but also the nucleosomal DNA. Here, we sought to find peptide-based binders of H3K36me3 reader PSIP1, which relies on DNA interactions to tightly bind H3K36me3 modified nucleosomes. We designed several peptides that mimic the nucleosomal context of H3K36me3 recognition by including negatively charged Glu-rich regions. Using a detailed NMR analysis, we find that addition of negative charges boosts binding affinity up to 50-fold while decreasing binding to the trimethyllysine binding pocket. Since screening and selection of compounds for reader domains is typically based solely on affinity measurements due to their lack of enzymatic activity, our case highlights the need to carefully control for the binding mode, in particular for the challenging case of H3K36me3 readers.
Production of Defense Phenolics in Tomato Leaves of Different Age
Authors: Dadáková Kateřina , Heinrichová Tereza , Lochman Jan , Kašparovský Tomáš
Published: October 2020

Phenolics play an essential role in the defense reaction of crop plants against pathogens. However, the intensity of their production induced by infection may differ during the life of a plant. Here, we identified age-related differences in phenolic biosynthesis in the pathosystem Solanum lycopersicum cv. Amateur and Pseudomonas syringae pv. tomato DC3000. We analyzed concentrations of total phenolics, phenolic profiles, and concentrations of selected phenolic acids. The influence of bacterial infection, together with leaf and plant age, was assessed. The changes in concentrations of caffeic acid, 4-hydroxybenzoic acid, and salicylic acid glucoside caused by infection were found to be influenced by age. In concrete, the increases in the concentrations of these metabolites were all evident only in young plants.
Recent Trends in Morphology-Controlled Synthesis and Application of Mesoporous Silica Nanoparticles
Authors: Pal Nabanita , Lee Jun-Hyeok , Cho Eun-Bum
Published: October 2020

The outstanding journey towards the investigation of mesoporous materials commences with the discovery of high surface area porous silica materials, named MCM-41 (Mobil Composition of Matter-41) according to the inventors’ name Mobile scientists in the United States. Based on a self-assembled supramolecular templating mechanism, the synthesis of mesoporous silica has extended to wide varieties of silica categories along with versatile applications of all these types in many fields. These silica families have some extraordinary structural features, like highly tunable nanoscale sized pore diameter, good Brunauer–Emmett–Teller (BET) surface areas, good flexibility to accommodate different organic and inorganic functional groups, metals etc., onto their surface. As a consequence, thousands of scientists and researchers throughout the world have reported numerous silica materials in the form of published articles, communication, reviews, etc. Beside this, attention is also given to the morphology-oriented synthesis of silica nanoparticles and their significant effects on the emerging fields of study like catalysis, energy applications, sensing, environmental, and biomedical research. This review highlights a consolidated overview of those morphology-based mesoporous silica particles, emphasizing their syntheses and potential role in many promising fields of research.
A Mix of Natural Bioactive Compounds Reduces Fat Accumulation and Modulates Gene Expression in the Adipose Tissue of Obese Rats Fed a Cafeteria Diet
Authors: Gibert-Ramos Albert , Martín-González Miguel Z. , Crescenti Anna , Salvadó M. Josepa
Published: October 2020

Scientists are focusing on bioactive ingredients to counteract obesity. We evaluated whether a mix containing grape seed proanthocyanidin extract (GSPE), anthocyanins, conjugated linoleic acid (CLA), and chicken feet hydrolysate (CFH) could reduce body fat mass and also determined which mechanisms in the white adipose tissue (WAT) and the brown adipose tissue (BAT) were affected by the treatment. The mix or vehicle (VH) were administered for three weeks to obese rats fed a cafeteria (CAF) diet. Biometric measures, indirect calorimetry, and gene expression in WAT and BAT were analyzed as was the histology of the inguinal WAT (IWAT). The individual compounds were also tested in the 3T3-L1 cell line. The mix treatment resulted in a significant 15% reduction in fat (25.01 ± 0.91 g) compared to VH treatment (21.19 ± 1.59 g), and the calorimetry results indicated a significant increase in energy expenditure and fat oxidation. We observed a significant downregulation of Fasn mRNA and an upregulation of Atgl and Hsl mRNA in adipose depots in the group treated with the mix. The IWAT showed a tendency of reduction in the number of adipocytes, although no differences in the total adipocyte area were found. GSPE and anthocyanins modulated the lipid content and downregulated the gene and protein levels of Fasn compared to the untreated group in 3T3-L1 cells. In conclusion, this mix is a promising treatment against obesity, reducing the WAT of obese rats fed a CAF diet, increasing energy expenditure and fat oxidation, and modifying the expression of genes involved in lipid metabolism of the adipose tissue.