bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiplex high-resolution melting assay for simultaneous detection of five key bacterial pathogens in urinary tract infections: A pilot study
Authors: Kafi Hossein , Emaneini Mohammad , Halimi Shahnaz , Rahdar Hossein Ali , Jabalameli Fereshteh , Beigverdi Reza
Published: December 2022

The diagnosis of urinary tract infections (UTIs) is usually based on the results of urine culture, but it is time-consuming, labor-intensive and has a low sensitivity. The aim of this study was to develop multiplex high-resolution melting assay (MHRM) for the simultaneous detection of five common bacterial pathogens (Escherichia coli, Klebsiella pneumoniae, Staphylococcus saprophyticus, Enterococcus faecalis, and group B streptococci (GBS)) directly from urine samples. A total of 287 urine specimens were evaluated by HRM assay and the results were compared with the conventional culture method. Five different melt curves generated and differentiated five bacterial pathogens. The detection limit of the MHRM assay was 1.5 × 103 CFU/ml for E. coli and K. pneumoniae and 1.5 × 102 CFU/ml for S. saprophyticus, E. faecalis and GBS. Compared to culture, the specificity of the MHRM assay ranged from 99.3 to 100%, and sensitivity 100% for all test pathogens. The MHRM assay developed in the current study might be functional tool for the diagnosis of UTIs and has the potential for direct detection of the organism in the clinical samples. Additionally, it creates results in less than 5 h, helping clinicians to start treatment with appropriate antimicrobial agents. This method could be a useful supplement to urine culture.
The relationship between response dynamics and the formation of confidence varies across the lifespan
Authors: Overhoff Helen , Ko Yiu Hong , Fink Gereon R. , Stahl Jutta , Weiss Peter H. , Bode Stefan , Niessen Eva
Published: December 2022

Accurate metacognitive judgments, such as forming a confidence judgment, are crucial for goal-directed behavior but decline with older age. Besides changes in the sensory processing of stimulus features, there might also be changes in the motoric aspects of giving responses that account for age-related changes in confidence. In order to assess the association between confidence and response parameters across the adult lifespan, we measured response times and peak forces in a four-choice flanker task with subsequent confidence judgments. In 65 healthy adults from 20 to 76 years of age, we showed divergent associations of each measure with confidence, depending on decision accuracy. Participants indicated higher confidence after faster responses in correct but not incorrect trials. They also indicated higher confidence after less forceful responses in errors but not in correct trials. Notably, these associations were age-dependent as the relationship between confidence and response time was more pronounced in older participants, while the relationship between confidence and response force decayed with age. Our results add to the notion that confidence is related to response parameters and demonstrate noteworthy changes in the observed associations across the adult lifespan. These changes potentially constitute an expression of general age-related deficits in performance monitoring or, alternatively, index a failing mechanism in the computation of confidence in older adults.
Case report: Treatment of anterior cerebral artery aneurysms with combined remodeling technique and flow diverter deployment through a dual lumen balloon catheter
Authors: Renieri Leonardo , Capasso Francesco , Nappini Sergio , Laiso Antonio , Capirossi Carolina , Limbucci Nicola
Published: December 2022

We present the technical aspects of embolization for two unruptured medium-sized aneurysms of the anterior cerebral artery treated with balloon-remodeling technique and loose coiling of the sac with the final deployment of a 0. 017-compatible flow diverter. Both procedures were performed with dual antiplatelet therapy premedication and under general anesthesia. The anatomy of the two aneurysms was similar with a wide neck and the presence of a collateral artery branching off it, which required the additional use of a compliant balloon in order to retain patency and avoid coil protrusion. After initial coiling, a nitinol flow-diverter was deployed through a coaxial dual lumen balloon microcatheter. Both these interventions encountered no complications, and the patient was discharged on day 2. At 6-month clinical and radiological follow-up, neither patient had neurological deficits, the aneurysms were both completely occluded, nor the stented arteries were patent along with their collateral branches.
Unraveling rare form of adult-onset NIID by characteristic brain MRI features: A single-center retrospective review
Authors: Li Fan , Wang Qi , Zhu Ying , Xiao Jiangxi , Gu Muliang , Yu Jiaxi , Deng Jianwen , Sun Wei , Wang Zhaoxia
Published: December 2022

Adult-onset neuronal intranuclear inclusion disease (NIID) is a rare neurodegenerative disorder with high clinical heterogeneity. Previous studies indicated that the high-intensity signals in the corticomedullary junction on diffusion-weighted imaging (DWI) on brain MRI, known as the “ribbon sign,” could serve as a strong diagnostic clue. Here we used the explorative approach to study the undiagnosed rate of adult-onset NIID in a single center in China via searching for the ribbon sign in picture archive and communication system (PACS) and report the clinical and radiological features of initially undiagnosed NIID patients.

Consecutive brain MRI of 21,563 adult individuals (≥18 years) in the PACS database in 2019 from a tertiary hospital were reviewed. Of them, 4,130 were screened out using the keywords “leukoencephalopathy” and “white matter demyelination.” Next, all 4,130 images were read by four neurologists. The images with the suspected ribbon sign were reanalyzed by two neuroradiologists. Those with the ribbon sign but without previously diagnosed NIID were invited for skin biopsy and/or genetic testing for diagnostic confirmation. The clinical features of all NIID patients were retrospectively reviewed.

Five patients with high-intensity in the corticomedullary junction on DWI were enrolled. Three patients were previously diagnosed with NIID confirmed by genetic or pathological findings and presented with episodic encephalopathy or cognitive impairment. The other two patients were initially diagnosed with limb-girdle muscular dystrophy (LGMD) with rimmed vacuoles (RVs) and normal pressure hydrocephalus (NPH) in one each. Genetic analysis demonstrated GGC repeat expansion in the NOTCH2NLC gene of both, and skin biopsy of the first patient showed the presence of intranuclear hyaline inclusion bodies. Thus, five of the 21,563 adult patients (≥18 years) were diagnosed with NIID. The distinctive subcortical high-intensity signal on DWI was distributed extensively throughout the lobes, corpus callosum, basal ganglia, and brainstem. In addition, T2-weighted imaging revealed white matter hyperintensity of Fazekas grade 2 or 3, atrophy, and ventricular dilation. Distinctive DWI hyperintensity in the junction between the gray and white matter can help identify atypical NIID cases. Our findings highly suggest that neurologists and radiologists should recognize the characteristic neuroimaging pattern of NIID.
Lipid storage myopathy due to late-onset multiple Acyl-CoA dehydrogenase deficiency with novel mutations in ETFDH: A case report
Authors: Tian Huihong , Zhong Yi , Liu Zhihua , Wei Liping , Yuan Yanbo , Zhang Yuhu , Wang Limin
Published: December 2022

Background

Lipid storage myopathy (LSM) is an autosomal recessive inherited lipid and amino metabolic disorder with great clinical heterogeneity. Variations in the electron transfer flavoprotein dehydrogenase (ETFDH) gene cause multiple acyl-CoA dehydrogenase deficiency (MADD), and have a manifestation of LSM. Muscle biopsy helps clarify the diagnosis of LSM, and next-generation sequencing (NGS) can be useful in identifying genomic mutation sites. The diagnosis of MADD contributes to targeted therapy.

Case presentation

We report on a teenager who appeared to have muscle weakness and exercise intolerance at the onset. Before the referral to our hospital, he was unsuccessfully treated with glucocorticoid for suspected polymyositis. The next-generation sequencing of the proband and his parents revealed heterozygous variations, c.365G>A (p.G122D) inherited from the father, c.176-194_176-193del, and c.832-316C>T inherited from the mother in the ETFDH gene. The tandem mass spectrometry identified the mutations to be pathogenic. However, his parents and his younger sister who were detected with a mutation of c.365G>A presented no clinical symptoms. This indicates that the combination of the three compound heterozygous mutations in ETFDH is significant. After MADD was diagnosed, a dramatic clinical recovery and biochemical improvement presented as riboflavin was given to the patient across a week, which further confirmed the diagnosis of MADD.

Conclusion

Our observations extend the spectrum of ETFDH variants in Chinese the population and reinforce the role of NGS in diagnosis of MADD. Early diagnosis and appropriate treatment of LSM lead to great clinical efficacy and avoid some lethal complications.
Impairment mechanism of nasal mucosa after radiotherapy for nasopharyngeal carcinoma
Authors: Fang Caishan , Zhong Yu , Chen Tengyu , Li Dan , Li Chunqiao , Qi Xiangjun , Zhu Junxia , Wang Ruizhi , Zhu Jinxiang , Wang Shunlan , Ruan Yan , Zhou Min
Published: December 2022

The nasal mucosa, which performs the crucial functions of filtering, humidifying and temperature regulation, is one of the most vulnerable areas of nasopharyngeal carcinoma (NPC) patients after radiotherapy (RT). Following RT, NPC patients experience a series of pathological changes in the nasal mucosa, ultimately leading to physiological dysfunction of the nasal epithelium. This article systematically reviews the clinical and pathological manifestations of RT-related nasal damage in NPC patients and summarizes the potential mechanism of damage to the human nasal epithelium by RT. Finally, we outline the current mechanistic models of nasal epithelial alterations after RT in NPC patients and provide additional information to extend the in-depth study on the impairment mechanisms of the nasal mucosa resulting from RT. We also describe the relationship between structural and functional alterations in the nasal mucosa after RT to help mitigate and treat this damage and provide insights informing future clinical and fundamental investigations.
Prognostic significance of sarcopenia and systemic inflammation for patients with renal cell carcinoma following nephrectomy
Authors: Liu Qiuchen , Yang Jiajian , Chen Xin , Yang Jiakang , Zhao Xiaojun , Huang Yuhua , Lin Yuxin , Pu Jinxian
Published: December 2022

Background

To clarify the prognostic effect of preoperative sarcopenia and systemic inflammation, and to develop a nomogram for predicting overall survival (OS) of patients with renal cell carcinoma (RCC) following partial or radical nephrectomy.

Methods

Patients with RCC following nephrectomy from the First Affiliated Hospital of Soochow University during January 2018 to September 2020 were included in this study. The relationship between sarcopenia and inflammatory markers was identified by logistic regression analysis. Then univariable Cox regression analysis, LASSO regression analysis and multivariable Cox regression analysis were analyzed sequentially to select the independent prognostic factors. Kaplan-Meier survival curves were applied to ascertain the prognostic value. Finally, the identified independent predictors were incorporated in a nomogram, which was internally validated and compared with other methods.

Results

A total of 276 patients were enrolled, and 96 (34.8%) were diagnosed with sarcopenia, which was significantly associated with neutrophil-to-lymphocyte ratio (NLR). Sarcopenia and elevated inflammation markers, i.e., NLR, platelet-to-lymphocyte ratio (PLR) and the modified Glasgow Prognostic Score (mGPS), were independent factors for determining the OS. The model had good discrimination with Concordance index of 0.907 (95% CI: 0.882–0.931), and the calibration plots performed well. Both net reclassification index (NRI) and integrated discriminant improvement (IDI) exhibited better performance of the nomogram compared with clinical stage-based, sarcopenia-based and integrated “NLR+PLR+mGPS” methods. Moreover, decision curve analysis showed a net benefit of the nomogram at a threshold probability greater than 20%.

Conclusions

Preoperative sarcopenia was significantly associated with NLR. A novel nomogram with well validation was developed for risk stratification, prognosis tracking and personalized therapeutics of RCC patients.
Construction and validation of a clinical predictive nomogram for intraductal carcinoma of the prostate based on Chinese multicenter clinical data
Authors: Yang YunKai , Zhang Wei , Wan LiJun , Tang ZhiLing , Zhang Qi , Bai YuChen , Zhang DaHong
Published: December 2022

Introduction

Intraductal carcinoma of the prostate (IDC-P) is a special pathological type of prostate cancer that is highly aggressive with poor prognostic outcomes.

Objective

To establish an effective predictive model for predicting IDC-P.

Methods

Data for 3185 patients diagnosed with prostate cancer at three medical centers in China from October 2012 to April 2022 were retrospectively analyzed. One cohort (G cohort) consisting of 2384 patients from Zhejiang Provincial People’s Hospital was selected for construction (Ga cohort) and internal validate (Gb cohort)of the model. Another cohort (I cohort) with 344 patients from Quzhou People’s Hospital and 430 patients from Jiaxing Second People’s Hospital was used for external validation. Univariate and multivariate binary logistic regression analyses were performed to identify the independent predictors. Then, the selected predictors were then used to establish the predictive nomogram. The apparent performance of the model was evaluated via externally validated. Decision curve analysis was also performed to assess the clinical utility of the developed model.

Results

Univariate and multivariate logistic regression analyses showed that alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), prostate specific antigen (PSA) and lactate dehydrogenase were independent predictors of IDC-P. Therefore, a predictive nomogram of IDC-P was constructed. The nomogram had a good discriminatory power (AUC = 0.794). Internal validation (AUC = 0.819)and external validation (AUC = 0.903) also revealed a good predictive ability. Calibration curves showed good agreement between the predicted and observed incidences of IDC-P.

Conclusion

We developed a clinical predictive model composed of alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), prostate specific antigen (PSA) and lactate dehydrogenase (LDH) with a high precision and universality. This model provides a novel calculator for predicting the diagnosis of IDC-P and different treatment options for patients at an early stage.
The integrative bioinformatics approaches to predict the xanthohumol as anti-breast cancer molecule: Targeting cancer cells signaling PI3K and AKT kinase pathway
Authors: Gupta Kartikey Kumar , Sharma Kamal Kant , Chandra Harish , Panwar Himalaya , Bhardwaj Nitin , Altwaijry Najla A. , Alsfouk Aisha A. , Dlamini Zodwa , Afzal Obaid , Altamimi Abdulmalik S. A. , Khan Shahanavaj , Mishra Abhay Prakash
Published: December 2022

Background

Breast cancer is the most common type of cancer in women, and vast research is being conducted throughout the world for the treatment of this malignancy by natural products using various computational approaches. Xanthohumol, a prenylated flavonoid, is known for its anticancer activity; however, the mechanism behind its action is still in the preliminary stage.

Methods

The current study aimed to analyze the efficacy of xanthohumol compared to the currently available anticancer drugs targeting phosphoinositide-3-kinase (PI3K), serine/threonine kinase (AKT) receptors, and human epidermal growth factor receptor 2 (HER2) for breast cancer treatment through in silico analysis.

Results

The result revealed that the target compound showed significant binding affinity to targets within the PI3K, AKT, and HER2 signaling pathways with a binding energy of −7.5, −7.9, and −7.9 kcal/mol, respectively. Further prediction studies were then made concerning this compound’s absorption, distribution, metabolism, and excretion (ADME) as well as drug-likeness properties, resulting in its oral bioavailability with only a single violation of Lipinski’s rule of five.

Conclusions

The finding revealed the ability of xanthohumol to bind with multiple cancer cell signaling molecules including PI3K, AKT kinase, and HER2. The current novel study opened the door to advancing research into the management and treatment of breast cancer.
Pharmacokinetics, metabolite profiling, safety, and tolerability of inhalation aerosol of 101BHG-D01, a novel, long-acting and selective muscarinic receptor antagonist, in healthy Chinese subjects
Authors: Gao Huaye , Li Jintong , Chen Xiaoping , Sun Zhanguo , Cui Gang , Cheng Minlu , Ding Li
Published: December 2022

101BHG-D01 is a novel, long-acting, selective muscarinic receptor antagonist for the treatment of chronic obstructive pulmonary disease (COPD). A single-site, randomized, double-blind, placebo-controlled and dose-escalation study of 101BHG-D01 inhalation aerosol was conducted to evaluate its pharmacokinetics, metabolite profiling, safety and tolerability following the single inhaled doses ranged from 20 to 900 μg in healthy Chinese subjects. After inhalation, 101BHG-D01 was absorbed rapidly into plasma with the time to maximum concentration about 5 min, and eliminated slowly with the terminal phase half-life about 30 h. The cumulative excretion rates of 101BHG-D01 in feces and urine were about 30% and 2%, respectively, which showed the study drug was mainly excreted in feces. The maximum drug concentration and area under the plasma concentration-time curve increased with dose escalation in the range of 20–600 μg, but their values increased out of proportion to the whole studied doses. The main metabolic pathways were loss of phenyl group and hydroxylation. No metabolite that presented at greater than 10 percent of total drug-related exposure was observed. 101BHG-D01 was safe and well tolerated after administration. The study results indicate that 101BHG-D01 is a good candidate for the treatment of COPD and enable further clinical development in subsequent studies in patients.

Clinical Trial Registration:http://www.chinadrugtrials.org.cn; Identifier: CTR20192058.
The synergistic activity of SBC3 in combination with Ebselen against Escherichia coli infection
Authors: Chen Hao , Lu Qianqian , An Haoyue , Li Juntong , Shen Shuchu , Zheng Xi , Chen Wei , Wang Lu , Li Jihong , Du Youqin , Wang Yueqing , Liu Xiaowen , Baumann Marcus , Tacke Matthias , Zou Lili , Wang Jun
Published: December 2022

Escherichia coli ranks as the number one clinical isolate in the past years in China according to The China Antimicrobial Surveillance Network (CHINET), and its multidrug-resistant (MDR) pathogenic strains account for over 160 million cases of dysentery and one million deaths per year. Here, our work demonstrates that E. coli is highly sensitive to the synergistic combination of SBC3 [1,3-Dibenzyl-4,5-diphenyl-imidazol-2-ylidene silver (I) acetate] and Ebselen, which shows no synergistic toxicity on mammalian cells. The proposed mechanism for the synergistic antibacterial effect of SBC3 in combination with Ebselen is based on directly inhibiting E. coli thioredoxin reductase and rapidly depleting glutathione, resulting in the increase of reactive oxygen species that cause bacterial cell death. Furthermore, the bactericidal efficacy of SBC3 in combination with Ebselen has been confirmed in mild and acute peritonitis mice. In addition, the five most difficult to treat Gram-negative bacteria (including E. coli, Acinetobacter baumannii, Enterobacter cloacae, Klebsiella pneumoniae, and Pseudomonas aeruginosa) are also highly sensitive to a synergistic combination of SBC3 and Ebselen. Thus, SBC3 in combination with Ebselen has potential as a treatment for clinically important Gram-negative bacterial infections.
Pharmacological effects of Bufei Jianpi granule on chronic obstructive pulmonary disease and its metabolism in rats
Authors: Yang Xin-Xin , Wang Shuai , Cui Lin-Lin , Li Tian-Jiao , Bai Gang , Bao Yong-Rui , Meng Xian-Sheng
Published: December 2022

This work was performed to determine the pharmacological effects of Bufei Jianpi granules on chronic obstructive pulmonary disease and its metabolism in rats.

Chronic obstructive pulmonary disease (COPD), ranked as the third leading cause of death worldwide, is seriously endangering human health. At present, the pathogenesis of COPD is complex and unclear, and the drug treatment mainly aims to alleviate and improve symptoms; however, they cannot achieve the purpose of eradicating the disease. Bufei Jianpi granule (BJG) is a Chinese medicine developed by the First Affiliated Hospital of Henan University of Traditional Chinese Medicine for treating COPD. This study focuses on the pharmacological effects of BJG on COPD and its metabolism in rats, aiming to provide a scientific basis for developing BJG against COPD. A total of 72 Sprague–Dawley (SD) rats were divided into the blank group, model group, positive control group, and BJG groups (2.36, 1.18, and 0.59 g/kg). Except for the blank group, rats in other groups were administered lipopolysaccharide (LPS) combined with smoking for 6 weeks to establish the COPD model. After another 6 weeks of treatment, the therapeutic effect of BJG on COPD rats was evaluated. In the BJG (2.36 g/kg) group, the cough condition of rats was significantly relieved and the body weight was close to that of the blank group. Compared with the mortality of 16.7% in the model group, no deaths occurred in the BJG (2.36 g/kg) and (1.18 g/kg) groups. The lung tissue damage in the BJG groups was less than that in the COPD group. Compared with the model group, MV, PIF, PEF, and EF50 in the BJG groups were observably increased in a dose-dependent manner, while sRaw, Raw, and FRC were obviously decreased. Also, the contents of IL-6, IL-8, TNF-α, PGE2, MMP-9, and NO in the serum and BALF were lowered dramatically in all BJG groups. All indicators present an obvious dose–effect relationship. On this basis, the UPLC-QTOF-MS/MS technology was used to analyze characteristic metabolites in rats under physiological and pathological conditions. A total of 17 prototype and 7 metabolite components were detected, and the concentration of most components was increased in the COPD pathologic state. It is suggested that BJG has a pharmacological effect in the treatment of COPD and the absorption and metabolism of chemical components of BJG in rats exhibited significant differences under physiological and pathological conditions.
Microbiota and their metabolites potentiate cancer immunotherapy: Therapeutic target or resource for small molecule drug discovery?
Authors: Du Peixin , Jing Jing , He Xiujing
Published: December 2022

Increasing evidence has proved that microbiota is not only the target of small molecule drugs but also an underexplored resource for developing small molecule drugs. Meanwhile, microbiota as a critical modulator of the immune system impacts the efficacy and toxicity of cancer immunotherapy. Harnessing microbiota or developing microbiota-derived medications provide novel therapeutic strategies to overcome resistance to cancer immunotherapy and immune-related adverse events (irAEs). In this review, we elucidate how microbiota and their metabolites impact anti-tumor immunity and immunotherapy efficacy and highlight the potential of microbiota and their metabolites as a resource for small molecule drug discovery. We further overview the current landscape of clinical trials evaluating the potential effect of microbiota and their metabolites on immunotherapy outcomes, presenting future trends in the field of microbiota-based therapies. Microbiota-based therapies are promising therapeutic options to promote therapeutic efficacy and diminish the toxicity of immunotherapy.
Environmental exposure and the role of AhR in the tumor microenvironment of breast cancer
Authors: Sweeney Colleen , Lazennec Gwendal , Vogel Christoph F. A.
Published: December 2022

Activation of the aryl hydrocarbon receptor (AhR) through environmental exposure to chemicals including polycyclic aromatic hydrocarbons (PAHs) and polychlorinated dibenzo-p-dioxins (PCDDs) can lead to severe adverse health effects and increase the risk of breast cancer. This review considers several mechanisms which link the tumor promoting effects of environmental pollutants with the AhR signaling pathway, contributing to the development and progression of breast cancer. We explore AhR’s function in shaping the tumor microenvironment, modifying immune tolerance, and regulating cancer stemness, driving breast cancer chemoresistance and metastasis. The complexity of AhR, with evidence for both oncogenic and tumor suppressor roles is discussed. We propose that AhR functions as a “molecular bridge”, linking disproportionate toxin exposure and policies which underlie environmental injustice with tumor cell behaviors which drive poor patient outcomes.
Dynamical models of acute respiratory illness caused by human adenovirus on campus
Authors: Zhang Wei , Ma Xia , Zhang Yongxin , Luo Xiaofeng
Published: December 2022

Acute respiratory illness caused by human adenovirus have been increasing in morbidity and mortality in recent years. Currently, isolation of symptomatic infected individuals is the primary means of controlling outbreaks in closed spaces such as schools and military camps. However, the disease is still spreading despite the implementation of control measures. To reveal the underlying mechanism of this phenomenon, we propose a dynamic model that considers invisible transmission and isolated confirmed cases. By calculating and analyzing the control reproduction number, it is found that asymptomatic infected individuals play an important role in the spread of the epidemic. Therefore, in the absence of specific vaccines, non-pharmaceutical interventions such as quarantine of exposed individuals are effective means to mitigate severity. The results show that the earlier the control of invisible transmission is implemented, the lower the peak and the shorter the duration of the outbreak. These findings will provide the theoretical basis and recommendations for prevention and control of human adenovirus transmission in closed spaces.
The role of KCC2 and NKCC1 in spinal cord injury: From physiology to pathology
Authors: Talifu Zuliyaer , Pan Yunzhu , Gong Han , Xu Xin , Zhang Chunjia , Yang Degang , Gao Feng , Yu Yan , Du Liangjie , Li Jianjun
Published: December 2022

The balance of ion concentrations inside and outside the cell is an essential homeostatic mechanism in neurons and serves as the basis for a variety of physiological activities. In the central nervous system, NKCC1 and KCC2, members of the SLC12 cation-chloride co-transporter (CCC) family, participate in physiological and pathophysiological processes by regulating intracellular and extracellular chloride ion concentrations, which can further regulate the GABAergic system. Over recent years, studies have shown that NKCC1 and KCC2 are essential for the maintenance of Cl− homeostasis in neural cells. NKCC1 transports Cl− into cells while KCC2 transports Cl− out of cells, thereby regulating chloride balance and neuronal excitability. An imbalance of NKCC1 and KCC2 after spinal cord injury will disrupt CI− homeostasis, resulting in the transformation of GABA neurons from an inhibitory state into an excitatory state, which subsequently alters the spinal cord neural network and leads to conditions such as spasticity and neuropathic pain, among others. Meanwhile, studies have shown that KCC2 is also an essential target for motor function reconstruction after spinal cord injury. This review mainly introduces the physiological structure and function of NKCC1 and KCC2 and discusses their pathophysiological roles after spinal cord injury.
Non-alcoholic fatty liver disease—A pilot study investigating early inflammatory and fibrotic biomarkers of NAFLD with alcoholic liver disease
Authors: Watt Joanne , Kurth Mary Jo , Reid Cherith N. , Lamont John V. , Fitzgerald Peter , Ruddock Mark W.
Published: December 2022

Introduction: Non-alcoholic fatty liver disease (NAFLD) is a condition where excess fat accumulates in the liver (hepatic steatosis) and there is no history of alcohol abuse or other secondary causes of chronic liver disease. NAFLD is a very common disorder, occurring in 25% of the global population. NAFLD is now the most common chronic liver disorder in Western countries. Liver biopsy is the gold standard for NAFLD diagnosis and staging; however, this is invasive, costly and not without risk. Biomarkers that could diagnose and stage disease would reduce the need for biopsy and allow stratification of patients at risk of progression to non-alcoholic steatohepatitis (NASH).

Methods: One hundred and thirty-five patients were involved in the study [N = 135: n = 34 controls; n = 26 simple steatosis; n = 61 NAFLD/NASH, and n = 14 alcoholic liver disease (ALD)]. Clinically diagnosed (ICD-10) patient serum samples were obtained from Discovery Life Sciences (US) along with clinical history. Samples were run in duplicate using high-sensitivity cytokine array I, immunoassays and ELISAs. In total, n = 20 individual biomarkers were investigated in this pilot study.

Results: Thirteen/20 (65%) biomarkers were identified as significantly different between groups; IFNγ, EGF, IL-1β, IL-6, IL-8, IL-10, TNFα, FABP-1, PIIINP, ST2/IL-33R, albumin, AST and ALT. Five/20 (25%) biomarker candidates were identified for further investigation; namely, three biomarkers of inflammation, IL-6, IL-8, and TNFα, and two biomarkers of fibrosis, PIIINP and ST2/IL-33R.

Discussion: Single biomarkers are unlikely to be diagnostic or predictive at staging NAFLD due to the complex heterogeneity of the disease. However, biomarker combinations may help stratify risk and stage disease where patients are averse to biopsy. Further studies comparing the 5 biomarkers identified in this study with current diagnostic tests and fibrotic deposition in liver tissue are warranted.
The uppermost monoterpenes improving Cinnamomum camphora thermotolerance by serving signaling functions
Authors: Xu Chenyi , Wang Bin , Luo Qingyun , Ma Yuandan , Zheng Tiefeng , Wang Yingying , Cai Yuyan , Zuo Zhaojiang
Published: December 2022

Terpenes serve important functions in enhancing plant thermotolerance. Cinnamomum camphora mainly has eucalyptol (EuL), camphor (CmR), linalool (LnL) and borneol (BeL) chemotypes basing on the uppermost monoterpenes. To reveal the thermotolerance mechanisms of these uppermost monoterpenes (eucalyptol, camphor, linalool, and borneol) in C. camphora, we surveyed the ROS metabolism and photosynthesis in the 4 chemotypes fumigated with the corresponding uppermost monoterpene after fosmidomycin (Fos) inhibiting monoterpene synthesis under high temperature at 38°C (Fos+38°C+monoterpene), and investigated the related gene expression in EuL and CmR. Meanwhile, the thermotolerance differences among the 4 uppermost monoterpenes were analyzed. In contrast to normal temperature (28°C), ROS levels and antioxidant enzyme activities in the 4 chemotypes increased under 38°C, and further increased in the treatment with Fos inhibiting monoterpene synthesis at 38°C (Fos+38°C), which may be caused by the alterations in expression of the genes related with non-enzymatic and enzymatic antioxidant formation according to the analyses in EuL and CmR. Compared with Fos+38°C treatment, Fos+38°C+monoterpene treatments lowered ROS levels and antioxidant enzyme activities for the increased non-enzymatic antioxidant gene expression and decreased enzymatic antioxidant gene expression, respectively. High temperature at 38°C reduced the chlorophyll and carotenoid content as well as photosynthetic abilities, which may result from the declined expression of the genes associated with photosynthetic pigment biosynthesis, light reaction, and carbon fixation. Fos+38°C treatment aggravated the reduction. In contrast to Fos+38°C treatment, Fos+38°C+monoterpene treatments increased photosynthetic pigment content and improved photosynthetic abilities by up-regulating related gene expression. Among the 4 uppermost monoterpenes, camphor showed strong abilities in lowering ROS and maintaining photosynthesis, while eucalyptol showed weak abilities. This was consistent with the recovery effects of the gene expression in the treatments with camphor and eucalyptol fumigation. Therefore, the uppermost monoterpenes can enhance C. camphora thermotolerance as signaling molecules, and may have differences in the signaling functions.
Effect of different planting pattern arrangements on soil organic matter and soil nitrogen content under a maize/soybean strip relay intercropping system
Authors: Te Xiao , Hassan Muhammad Jawad , Cui Kuoshu , Xiao Jiahui , Aslam Muhammad Naveed , Saeed Amjad , Yang Wenyu , Ali Safdar
Published: December 2022

Assessing the spatial distribution of organic matter and total nitrogen in soil is essential for management and optimum utilization of fertilizers. Therefore, the present field experiment was conducted to evaluate the impact of different planting pattern arrangements on the spatial distribution of soil total nitrogen and organic matter content under a maize/soybean strip relay intercropping system. The planting was arranged in a manner such that soil sampling could be done from continuous maize/soybean relay strip intercropping (MS1), maize/soybean relay strip intercropping in rotation (MS2), traditional maize/soybean intercropping (MS3), sole maize (M), sole soybean (S), and fallow land (FL) from 2018 to 2020. The results showed significant variations for soil organic matter and total nitrogen content under different planting pattern arrangements of maize and soybean in the strip relay intercropping system. Across all systems, the highest soil organic matter (29.19 g/kg) and total nitrogen (10.19 g/kg) were recorded in MS2. In contrast, the lowest soil organic matter (1.69 g/kg) and total nitrogen (0.64 g/kg) were observed in FL. Soil organic matter and total nitrogen in MS2 increased by 186.45% and 164.06%, respectively, when compared with FL. Soil organic matter and total nitrogen in MS2 increased by 186.45% and 164.06%, respectively, when compared with FL. Furthermore, under MS2, the spatial distribution of soil organic matter was higher in both maize and soybean crop rows as compared with other cropping patterns, whereas the soil total nitrogen was higher under soybean rows as compared with maize in all other treatment. However, correlation analysis of the treatments showed variations in organic matter content. It can be concluded that different planting patterns can have varying effects on soil organic matter and total nitrogen distribution under the strip relay intercropping system. Moreover, it is recommended from this study that MS2 is a better planting pattern for the strip relay intercropping system, which can increase the spatial distribution of soil organic matter and total nitrogen, thereby improving soil fertility, C:N ratio, and crop production. This study will serve as a foundation towards the scientific usage of chemical fertilizers in agricultural sector.
The effects of grammatical gender on the processing of occupational role names in Slovene: An event-related potential study
Authors: Mikić Ljubi Jasna , Matkovič Andraž , Bon Jurij , Kanjuo Mrčela Aleksandra
Published: December 2022

The event-related potential method has proven to be a useful tool for studying the effects of gender information in language. Studies have shown that mismatch between the antecedent and the following referent triggers two ERP components, N400 and P600. In the present study, we investigated how grammatical gender affects the mental representation of the grammatical subject. A match-mismatch paradigm was used to investigate how masculine grammatical gender and gender-balanced forms (the explicit mention of masculine and feminine forms as word pairs) as role nouns affect the processing of the referent in Slovenian. The morphological complexity of Slovenian language required the use of anaphoric verbs instead of nouns/pronouns, on which previous research was based. The results showed that following both the gender-balanced and the masculine generic forms, P600 (but not N400) was observed in response to the feminine verb but not to the masculine verb. The P600 amplitude was smaller in the case of the gender-balanced form than in the case of the masculine generic form only. We have concluded that gender-balanced forms are more open to feminine continuations than masculine generic forms. This is the first ERP study in Slovenian to address the effects of processing grammatical gender, thus contributing to existing research on languages with grammatical gender. The great strength of the study is that it is one of the first ERP studies to test the mental inclusivity of gender-balanced forms.
Preschoolers’ attention to and learning from on-screen characters that vary by effort and efficiency: An eye-tracking study
Authors: Choi Koeun , Schlesinger Molly A. , Franchak John M. , Richert Rebekah A.
Published: December 2022

Prior findings are mixed regarding the extent to which children understand others’ effort in early childhood. Especially, little is known about how character effort impacts children’s selective attention and learning. This study examined preschoolers’ visual attention to and learning from two on-screen characters: One character exerting high effort with low efficiency and another character exerting low effort with high efficiency in solving problems successfully. Children between 3.5 and 6.5 years of age (N = 70) watched a video of the two on-screen characters successfully solving problems. Children’s eye movements were recorded during viewing. Each of the two on-screen characters consistently displayed either high effort/low efficiency or low effort/high efficiency to solve four problems (familiarization). For the final problem (testing), the two characters exerted the same level of effort as each other and used unique solutions to solve the problem. Children then solved the final problem themselves using real objects. Children could selectively use either character’s solution demonstrated in the video. Lastly, children explicitly judged how good the characters were at solving problems. Younger children were more likely to use the solution demonstrated by the character with high effort/low efficiency, whereas older children were more likely to use the solution provided by another character with low effort/high efficiency. Younger children allocated more attention to the high effort/low efficiency character than the low effort/high efficiency character, but this pattern was modified by age such that children’s gaze to the low effort/high efficiency character increased with age. Children’s explicit credibility judgments did not differ by character or child age. The findings are discussed with respect to preschoolers’ understanding of effort and implications for children’s learning from screen media.
Modeling personality antecedents and second language self-efficacy constructs with emerging adults in Japan: Domain-specific matching for assessing global competence in applied contexts
Authors: Kabir Russell Sarwar , Kramer Brandon , Koike Mayu , Sponseller Aaron C.
Published: December 2022

Introduction

Research on self–efficacy in intercultural communication (SEIC) provided validity evidence for second language (L2) self-efficacy domains. However, it lacked (1) an analysis of individual differences in personality as antecedents, (2) divergent validity from anxiety variables (i.e., foreign language classroom anxiety; FLCA), and (3) disambiguation from speaking (S-SE) and listening (L-SE) skill-specific self-efficacy types.

Methods

We conducted structural equation modeling of L2 self-efficacy and anxiety as response variables predicted by the Big Five model of personality in the context of Japanese undergraduate students at three university sites (n = 373), and a geographically diverse online survey of emerging adults (n = 1,326) throughout Japan.

Results

The final model for the nationally representative sample showed that SEIC was predicted by all identified personality factors. Differentially supported paths were observed linking L-SE with Conscientiousness (β = 0.24) and Extraversion (β = 0.16), and S-SE with Extraversion (β = 0.24) and Neuroticism (β = −0.12). The fear of failure factor of FLCA was predicted positively by Neuroticism (β = 0.25) and, surprisingly, Conscientiousness (β = 0.10), and negatively by Extraversion (β = −0.13). Relationships to Openness to Experience were only supported for SEIC (β = 0.17) and S-SE (β = 0.12).

Discussion

These findings provide specificity matching for personality and L2 self-efficacy domains as empirical advances for assessing global competence within the context of Japan. Implications for cultural influences on self-efficacy and applied educational practices in language and intercultural learning are discussed.
Wellbeing in line managers during mandatory working from home: How work and personal factors combine
Authors: van Gelder Marco , van Veldhoven Marc , van de Voorde Karina
Published: December 2022

The pandemic, particularly the aspect of forced working from home, has had a major impact on the workforce. Previous studies show that line managers have also experienced severe mental strain during this period. Since it is expected that hybrid working will be more the new normal than the exception in future, this study further examined line managers' work-related wellbeing in terms of engagement and exhaustion. Following the job characteristics model (JCM), we explore the mediating role of meaningful work between workplace innovation before the pandemic and line managers' work-related wellbeing during forced working from home. The underlying idea is that organizations that already adopted workplace innovation practices before the pandemic, give teams and employees more control, thus allowing a more meaningful role for line managers, which positively impacts line managers' work-related wellbeing during the pandemic. In addition, building upon Job Demands-Resources (JD-R) Theory and the role of personal resources therein, we explore digital leadership skills and work–life segmentation preference as moderators between meaningful work and work-related wellbeing. Our findings show that workplace innovation is positively associated with engagement via its effect on meaningful work, but not associated with exhaustion. Second, we found that work–life segmentation preference amplifies the relation between meaningful work and engagement (positive link) as well as exhaustion (negative link). This indicates that line managers with a high work–life segmentation preference who have a low score on meaningful work, experience less engagement and more exhaustion than line managers with a high score on meaningful work when working from home. No support was found for the moderation of digital leadership skills in the relationship between meaningful work, engagement, and exhaustion. Based on these results, we discuss implications for research and we provide practice recommendations.
The relationship between social support and goal pursuit among Chinese college students: The mediating role of just-world beliefs
Authors: Chen Dan , Miao Xiaolong , Ma Yuning , Tang Yulong
Published: December 2022

Based on the self-determination theory, this study examined the mechanisms underlying the influence of social support on goal pursuit among college students and focused on the mediating role played by just-world beliefs. The Aspiration Index Scale, Just-World Beliefs Scale, and Perceived Social Support Scale were used to assess relationships among 424 college students’ just-world beliefs, social support, and goal pursuit; additionally, the underlying mechanisms of those relationships were examined. The results showed that (1) there was a significant positive correlation between social support, just-world beliefs, and goal pursuit; (2) social support had a significant positive influence on goal pursuit; (3) just-world beliefs played a fully mediating role in the influence of social support on goal pursuit, with a mediating effect of 36.38%; and (4) personal just-world beliefs rather than general just-world beliefs fully mediated the effect of social support on goal pursuit, with a mediating effect of 39.00%. In conclusion, we found that for Chinese college students, social support has a significant positive effect on goal pursuit, and personal just-world beliefs play a fully mediating role in the effect of social support on goal pursuit.
On the relationships linking intrinsic and extrinsic sense of freedom with pro-environmental attitudes. Synergic and buffering effects of the identification with all humanity
Authors: Urbańska Beata , Radkiewicz Piotr , Uram Patrycja
Published: December 2022

This study aimed to examine whether the individual way of understanding freedom is related to pro-environmental attitudes. This idea has not been studied before. In the paper, the authors examined whether understanding freedom as extrinsic (absolute and unconditional) was related to a decrease in environmental concern and pro-environmental behavior, while understanding it as intrinsic (conditional, limited by the needs of other people) had the opposite effect. Another set of hypotheses concerned the moderating role of identification with all humanity (IWAH). The authors hypothesized that in people with a high level of IWAH, the positive relationship between intrinsic freedom and pro-environmental attitudes was stronger, and the negative relationship between extrinsic freedom and pro-environmental attitudes was weaker compared to people with a low level of IWAH. The study was conducted on a sample of 773 Polish young adults (18–29 years) using a professional research panel. The results provide empirical evidence that intrinsic and extrinsic way of understanding freedom is related to environmental concern and pro-environmental behavior. Moreover, the hypothesis concerning the moderating role of IWAH was confirmed. These results contribute to a better understanding of the factors that determine commitment to climate protection.