bluuebunny/crossref_metadata_2025 huggingface dataset:
Sweetening with Agavins: Its Impact on Sensory Acceptability, Physicochemical Properties, Phenolic Composition and Nutraceutical Potential of Oak Iced Tea
Authors: Araiza-Alvarado Aylín , Álvarez Saúl Alberto , Gallegos-Infante José Alberto , Sánchez-Burgos Jorge Alberto , Rocha-Guzmán Nuria Elizabeth , González-Herrera Silvia Marina , Moreno-Jiménez Martha Rocío , González-Laredo Rubén Francisco , Cervantes-Cardoza Verónica
Published: February 2025

Oak infusions enriched with agavins may offer nutraceutical benefits in the development of iced teas. This study evaluated infusions of Quercus sideroxyla and Quercus eduardii leaves formulated with different concentrations of agavins (0, 2, 6, and 10%), analyzing their physicochemical and sensory properties, chemical stability, and antioxidant capacity. The incorporation of agavins resulted in substantial modifications to physicochemical parameters, including pH, titratable acidity, and soluble solids, thereby enhancing product stability and consistency. Notable distinctions were observed between the two species with respect to their acidogenic response and soluble solid concentration. Interactions between agavins and phenolic compounds, as discerned by UPLC-PDA-ESI-MS/MS and FT-IR, exerted a significant influence on bioactivity of the phenolic constituents, thereby affecting the nutraceutical potential of the infusions. These interactions, facilitated by hydrogen bonds, led to reduction in phenolic acids, such as quinic acid (↓ 43%), and alteration in antioxidant capacity at high concentrations of agavins. The findings underscore the significance of meticulously designing balanced formulations that optimize chemical stability, functionality, and sensory acceptance, thereby ensuring the quality of the final product.
Evaluation of Effect of Dietary Supplementation with Microencapsulated Hydrolyzed Tannins on Growth, Slaughter Performance, Meat Quality, and Lipid Metabolism of Zhongshan Shelducks
Authors: Tian Zhimei , Tian Zhengwei , Yin Yingshan , Wu Yongmei , Li Zhenyuan , Liang Qiaohua , Yu Miao , Cui Yiyan , Ma Xianyong , Peng Guanghui , Li Zhenming
Published: February 2025

This study investigated the effects of microenapsulated hydrolyzed tannins (MHTs) on the growth performance and meat quality of Zhongshan shelducks. A total of 288 healthy Zhongshan shelducks with an average initial weight of 1790.27 ± 0.14 kg were randomly divided into four groups through a 56 d experiment period and were fed a basal diet supplemented with 0 (CON), 400, 800, and 1600 mg/kg MHTs, respectively. Results showed that 400 and 800 mg/kg MHTs improved the final body weight, average daily gain, glutathione peroxidase activity, and total antioxidant capacity compared to CON (p ≤ 0.05). The diet supplemented with 400 mg/kg MHTs decreased shear force and 800 mg/kg MHTs increased the yield of pectoralis major muscle compared to CON (p ≤ 0.05). Dietary MHTs increased inosine monophosphate content and decreased percentage C14:0 content in meat; however, the b*45 min value, 48 h drip loss, and shear force were increased but the percentage intramuscular fat (IMF) content was decreased in pectoralis major muscle with the increase in MHTs (p ≤ 0.05). Compared to CON, 400 and 800 mg/kg MHTs increased the percentage content of IMF, C18:1n-9, C18:2n-6, monounsaturated fatty acids, polyunsaturated fatty acids, and unsaturated fatty acids in pectoralis major muscle (p ≤ 0.05). Furthermore, 400 and 800 mg/kg MHTs improved the lipid metabolism of IMF deposition, fatty acid uptake, and adipogenesis by activating the peroxisome proliferator-activated receptor gamma pathway to regulate fatty acid synthetase and lipoprotein lipase genes. In conclusion, diets supplemented with 400 and 800 mg/kg MHTs could improve growth, meat quality, antioxidant capacity, and lipid metabolism in Zhongshan shelducks.
Cross-Cultural Comparison (13 Countries) of Consumers’ Willingness to Eat Specific Insect Powders in Five Food Types
Authors: Park Suyeon , Chambers Edgar , Lee Jeehyun
Published: February 2025

By 2050, the world’s population will rise to 9 billion, which implies that it is necessary to double protein production. We should consider more sustainable, alternative forms of protein. A solution to this is the use of insects, which offer high levels of protein and require less water than poultry, pork, and beef production. The objective of this study was to evaluate 13 countries’ consumer perceptions regarding the willingness to eat specific types of insects as powdered ingredients in five food types. An online survey was conducted using Check All That Apply (CATA) to assess consumer perceptions across 13 countries. Approximately 630 consumers in each country were surveyed (total n > 8100). The CATA data were analyzed using Cochran’s Q test, which showed highly significant differences among countries. The willingness to eat insects varied by country, food type, and insect species. The results of this study can be used to understand consumers’ perceptions of insects and offer an indicator that can be used when developing insect-containing foods in the future.
Construction of an Efficient Engineered Strain for Chaetoglobosin A Bioresource Production from Potato Starch Industrial Waste
Authors: Zhang Kai , Zhao Shanshan , Wang Zhengran , Cheng Ming , Wang Wan , Yang Qian
Published: February 2025

Chaetoglobosin A (CheA), a typical structure of the cytochalasin family, exhibits outstanding efficacy against a variety of tumor cells and plant pathogens. However, its low yield and high production cost are major obstacles limiting its wide application. In order to increase CheA yield, an engineered strain was established by overexpressing CgMfs, the gene encoding the MFS family’s efflux pump, on chassis cells lacking CgXpp1, which have been shown to act as a negative regulator of CheA biosynthesis. As expected, the engineered strain significantly boosted CheA production from 63.19 to 265.93 mg/L after incubation in PDA medium for 10 d, whereas the yield of the engineered strain was remarkably enhanced 2.93-fold compared with the wild type, following 10 d of cultivation utilizing potato starch industrial waste. The addition of metal ions had a positive effect on CheA production, with Cu2+ being the most effective and improving production to 176.92 mg/L. The optimal fermentation conditions were determined by response surface optimization, and under the optimal conditions, the engineered strain could stably produce CheA with a yield of 197.58 mg/L. This study provided the conditions for reducing production costs while increasing CheA production, as well as new strategies and insights for the production of the target compound.
Shoots Regeneration in Brigitta and Duke Blueberry Cultivars from Different Encapsulated Vegetative Propagules
Authors: Regni Luca , Cesarini Arianna , Calisti Silvia , Proietti Primo , Micheli Maurizio
Published: February 2025

Synthetic seed technology, an advanced in vitro propagation method, combines the benefits of micropropagation with the practicality of zygotic seeds, offering an efficient solution for the handling, storage, and transportation of plant propagules. This study represents the first investigation of the role of explant type in blueberry encapsulation. In particular, three non-embryogenic propagules (basal node, median node, and shoot tip) were evaluated in the Brigitta and Duke blueberry cultivars. The artificial endosperm consists of Woody Plant Medium (WPM) macro- and micro-nutrients and Murashige and Skoog vitamins at half concentration, inositol (5 g L−1), Indole-3-butyric acid (IBA) (0.005 mg L−1), zeatin (0.25 mg L−1), and sucrose (50 g L−1). Forty-five days after sowing in in vitro conditions, the encapsulated shoot tips showed better results than basal and median nodes in several parameters, including viability, shoot length, and fresh shoot weight, in both cultivars. In both cultivars, none of the encapsulated propagule types developed roots. These results underscore the advantages of using shoot tips in encapsulation technology for blueberries and provide valuable insights for optimizing encapsulation protocols to improve propagation efficiency.
Life Cycle Greenhouse Gas Emissions Analysis of the Chlor-Alkali Process and By-Product Hydrogen in the United States
Authors: Vyawahare Pradeep , Sun Pingping , Young Ben , Bafana Adarsh , Kim Taemin , Hawkins Troy R. , Elgowainy Amgad
Published: February 2025

Hydrogen is considered a key energy carrier for which interest has grown over recent years. Chlor-alkali plants in the United States (U.S.) can potentially recover and supply the by-product hydrogen at scale. However, there is a scarcity of standard analysis for energy use and emissions associated with products from chlor-alkali plants owing to lack of data and variations in chlor-alkali plant technology and operation. A rigorous life cycle analysis (LCA) is needed to quantify the emissions of by-product hydrogen and other products from chlor-alkali plants. In this study, we performed well-to-gate (WTG) emissions analysis of chlor-alkali products based on U.S. plant operating data gathered from the U.S. Environmental Protection Agency’s (EPA’s) Chemical Data Reporting database, the U.S. Energy Information Administration survey EIA-923 form, and the EPA’s Greenhouse Gas Reporting Program. We performed process-level mass allocation to allocate energy use and emissions to the chlor-alkali products. This study shows that the by-product hydrogen has WTG CO2 emissions of 1.3–1.9 kgCO2/kg H2 for plants without combined heat and power (non-CHP) and 1.5–2.4 kgCO2/kg H2 for plants with combined heat and power (CHP). Furthermore, we identified that electricity upstream emissions are the key driver affecting the emissions of by-product hydrogen from non-CHP plants, while CHP emissions can be reduced by electricity export to grids with higher carbon intensity (CI). Finally, the study shows that chlor-alkali plants in the U.S. can potentially meet up to 320 kilotons of hydrogen demand (approximately 3% of total demand) annually.
Analysis Between Green Hydrogen and Other Financial Assets: A Multi-Scale Correlation Approach
Authors: Pereira Eder J. A. L. , Anjos Letícia S. , Ferreira Paulo , Quintino Derick , Ett Gerhard , Murari Thiago B.
Published: February 2025

Improvements in quality of life, new technologies and population growth have significantly increased energy consumption in Brazil and around the world. The Paris Agreement aims to limit global warming and promote sustainable development, making green hydrogen a fundamental option for industrial decarbonization. Green hydrogen, produced through the electrolysis of water using renewable energy, is gaining traction as a solution to reducing carbon emissions, with the global hydrogen market expected to grow substantially. This study applies the ρDCCA method to evaluate the cross-correlation between the green hydrogen market and various financial assets, including the URTH ETF, Bitcoin, oil futures, and commodities, revealing some strong positive correlations. It highlights the interconnection of the green hydrogen market with developed financial markets and digital currencies. The cross-correlation between the green hydrogen market and the index representing global financial markets presented a value close to 0.7 for small and large time scales, indicating a strong cross-correlation. The green hydrogen market and Bitcoin also presented a cross-correlation value of 0.4. This study provides valuable information for investors and policymakers, especially those concerned with achieving sustainability goals and environmental-social governance compliance and seeking green assets to protect and diversify various traditional investments.
Detection of Antibiotic-Resistance Genes in Drinking Water: A Study at a University in the Peruvian Amazon
Authors: Chayña Euclides , Ferro Pompeyo , Morales-Rojas Eli , Ferro-Gonzales Ana , Nuñez Jorge , Pedraza Edwaldo , Florindes Jorge , Ferro-Gonzales Polan
Published: February 2025

This study investigated the presence of antibiotic-resistance genes in drinking water consumed by the university community in the Peruvian Amazon. Water samples were collected from three primary sources: inflow from the distribution network, a storage cistern, and an underground intake. Conventional PCR was employed to detect genes associated with resistance to erythromycin (ermC), ampicillin (amp), ciprofloxacin (QEP), multidrug resistance (marA), and specific multidrug resistance in E. coli (qEmarA). Physicochemical analysis revealed compliance with most regulatory standards; however, groundwater samples showed lead concentrations exceeding legal limits (0.72 mg/L) and lacked residual chlorine. All sampling points tested positive for the evaluated resistance genes, demonstrating the widespread dissemination of resistance factors in drinking water. Contrary to initial expectations, resistance genes were also prevalent in treated sources. These findings reveal a critical public health risk for the university community, emphasising the need for effective disinfection systems and robust monitoring protocols to ensure water safety. The presence of these resistance genes in water is a critical public health concern as it can facilitate the spread of resistant bacteria, reducing the effectiveness of medical treatments and increasing the risk of infections that are difficult to control.
High Mobility Group A1 Chromatin Keys: Unlocking the Genome During MPN Progression
Authors: Resar Linda M. S. , Luo Li Z.
Published: February 2025

Patients with chronic, indolent myeloproliferative neoplasms (MPNs) are at risk for transformation to highly lethal leukemia, although targetable mechanisms driving progression remain elusive. We discovered that the High Mobility Group A1 (HMGA1) gene is up-regulated with MPN progression in patients and required for evolution into myelofibrosis (MF) or acute myeloid leukemia (AML) in preclinical models. HMGA1 encodes the HMGA1 epigenetic regulators that modulate the chromatin state during embryogenesis and tissue regeneration. While HMGA1 is silenced in most differentiated cells, it becomes aberrantly re-expressed in JAK2 mutant (JAK2-V617F) MPN, with the highest levels after transformation to secondary MF or AML. Here, we review recent work highlighting HMGA1 function in MPN progression. Though underlying mechanisms continue to emerge, increasing evidence suggests that HMGA1 functions as a “chromatin key” required to “unlock” regions of the genome involved in clonal expansion and progression in MPN. Together, these findings illuminate HMGA1 as a driver of MPN progression and a promising therapeutic target.
The Role of Primary Cilia in Modulating the Luteinization Process of Ovarian Granulosa Cells in Mice
Authors: Long Xiaochuan , Min Xiayu , Xiao Xinyao , Wu Yao , Yang Zengming , Wen Xin
Published: February 2025

The corpus luteum is the principal progesterone-secreting gland, while primary cilia function as pivotal organelles in intercellular signal transduction. Together, they play an essential role in the establishment and maintenance of pregnancy. However, the mechanisms underlying the role of primary cilia in granulosa cell luteinization in mouse ovaries remain poorly understood. This study discovered the regularity of primary cilia in mouse ovaries and revealed the role of primary cilia in regulating progesterone synthesis in luteinized granulosa cells. In vivo test results showed that the expression of primary cilia was obvious in the corpus luteum. The secretion of P4 in mice was significantly increased at 6, 12, 24, 48, and 72 h. The secretion of P4 and the expressions of luteinization markers (STAR, 3β-HSD) and primary ciliate proteins (IFT88, Arl13B) were significantly up-regulated at different time points (6, 12, 24 h and 24, 48, 72 h), and the INS group was significantly higher than the LH group and the control. In vitro test results showed that the follicular granulosa cells were luteinized under INS, the length and number of primary cilia increased, and the secretion of progesterone increased. The expression levels of STAR and 3β-HSD of the primary cilia marker Arl13B and luteinization markers were increased, while the expression levels of CYP19A1 were decreased. Ciliobrevin A (CBA) and Y-27632 2HCl were used to regulate the expression of primary cilia. The results showed that after CBA treatment, the expression level of cilia protein Arl13B decreased, and the secretion level of P4 and the expression levels of STAR and 3β-HSD decreased, indicating that the level of luteinization decreased. Conversely, after inducing ciliogenesis with Y-27632 2HCl, the results were the opposite of those observed with CBA treatment. In conclusion, our study demonstrates that primary cilia regulate the expression of steroidogenic enzymes, thereby promoting progesterone secretion by granulosa cells in mice and ensuring proper luteinization.
Acid Sphingomyelinase Regulates AdipoRon-Induced Differentiation of Arterial Smooth Muscle Cells via TFEB Activation
Authors: Li Xiang , Zhao Wei , Wang Zhengchao , Moura Alexandra K. , Roudbari Kiana , Zuo Rui , Hu Jenny Z. , Wang Yun-Ting , Li Pin-Lan , Zhang Yang
Published: February 2025

AdipoRon is a selective adiponectin receptor agonist that inhibits vascular remodeling by promoting the differentiation of arterial smooth muscle cells (SMCs). Our recent studies have demonstrated that activation of TFEB and its downstream autophagy–lysosomal signaling contribute to adipoRon-induced differentiation of SMCs. The present study was designed to examine whether acid sphingomyelinase (ASM; gene symbol Smpd1) is involved in mediating adipoRon-induced activation of TFEB–autophagy signaling and inhibition of proliferation/migration in arterial SMCs. Our results showed that adipoRon induced ASM expression and ceramide production in Smpd1+/+ SMCs, which were abolished in Smpd1−/− SMCs. Compared to Smpd1+/+ SMCs, Smpd1−/− SMCs exhibited less TFEB nuclear translocation and activation of autophagy signaling induced by adipoRon stimulation. SMC differentiation was further characterized by retarded wound healing, reduced proliferation, F-actin reorganization, and MMP downregulation. The results showed that Smpd1−/− SMCs were less responsive to adipoRon-induced differentiation than Smpd1+/+ SMCs. Mechanistically, adipoRon increased the expression of protein phosphatases such as calcineurin and PP2A in Smpd1+/+ SMCs. The calcineurin inhibitor FK506/cyclosporin A or PP2A inhibitor okadaic acid significantly attenuated adipoRon-induced activation of TFEB–autophagy signaling. In addition, adipoRon-induced expressions of calcineurin and PP2A were not observed in Smpd1−/− SMCs. However, activation of calcineurin by lysosomal TRPML1-Ca2+ channel agonist ML-SA1 rescued the activation of TFEB–autophagy signaling and the effects of adipoRon on cell differentiation in Smpd1−/− SMCs. Taken together, these data suggested that ASM regulates adipoRon-induced SMC differentiation through TFEB activation. This study provided novel mechanistic insights into the therapeutic effects of adipoRon on TFEB signaling and pathological vascular remodeling.
Shape Matters: The Utility and Analysis of Altered Yeast Mitochondrial Morphology in Health, Disease, and Biotechnology
Authors: Kichuk Therese , Avalos José L.
Published: February 2025

Mitochondria are involved in a wide array of critical cellular processes from energy production to cell death. The morphology (size and shape) of mitochondrial compartments is highly responsive to both intracellular and extracellular conditions, making these organelles highly dynamic. Nutrient levels and stressors both inside and outside the cell inform the balance of mitochondrial fission and fusion and the recycling of mitochondrial components known as mitophagy. The study of mitochondrial morphology and its implications in human disease and microbial engineering have gained significant attention over the past decade. The yeast Saccharomyces cerevisiae offers a valuable model system for studying mitochondria due to its ability to survive without respiring, its genetic tractability, and the high degree of mitochondrial similarity across eukaryotic species. Here, we review how the interplay between mitochondrial fission, fusion, biogenesis, and mitophagy regulates the dynamic nature of mitochondrial networks in both yeast and mammalian systems with an emphasis on yeast as a model organism. Additionally, we examine the crucial role of inter-organelle interactions, particularly between mitochondria and the endoplasmic reticulum, in regulating mitochondrial dynamics. The dysregulation of any of these processes gives rise to abnormal mitochondrial morphologies, which serve as the distinguishing features of numerous diseases, including Parkinson’s disease, Alzheimer’s disease, and cancer. Notably, yeast models have contributed to revealing the underlying mechanisms driving these human disease states. In addition to furthering our understanding of pathologic processes, aberrant yeast mitochondrial morphologies are of increasing interest to the seemingly distant field of metabolic engineering, following the discovery that compartmentalization of certain biosynthetic pathways within mitochondria can significantly improve chemical production. In this review, we examine the utility of yeast as a model organism to study mitochondrial morphology in both healthy and pathologic states, explore the nascent field of mitochondrial morphology engineering, and discuss the methods available for the quantification and classification of these key mitochondrial morphologies.
Synthesis of the Titanium Oxides Using a New Microwave Discharge Method
Authors: Mogildea Marian , Mogildea George , Zgura Sorin I. , Chiritoi Gabriel , Ionescu Cristian , Craciun Valentin , Prepelita Petronela , Mihailescu Natalia , Paraschiv Alexandru , Vasile Bogdan Stefan , Constantinescu Catalin Daniel
Published: February 2025

This research highlights the different behaviors of titanium (Ti) wires under the action of 500 W and 800 W microwave power levels. Following the interaction between microwaves and a titanium wire placed in the node of the (TM011—transverse magnetic mode) waveguide in air at atmospheric pressure, plasma was generated. Using optical emission spectroscopy technique it was observed that during plasma generation at 500 W and 800 W microwaves powers, metallic ions, and gas ions were created, and the plasmas fulfilled the local thermodynamic equilibrium (LTE) conditions. The XRD analysis showed that on the surface of the Ti wire exposed to 500 W microwave power a mixture of titanium dioxide (TiO2) and titanium oxide (TiO) grew, while the Ti wire exposed to 800 W microwave power was completely vaporized and a mixture of TiO2 and TiO nanoparticles was synthesized. The SEM analysis showed that the dimensions of the titanium oxide (TiOx) nanoparticles generated by both microwave discharges ranged from 5 nm to 200 nm. The results of EDS analysis showed that the power of microwaves plays an important role in quantitative conversion from Ti wire into a TiOx mixture. The TEM analysis indicates that most of the nanoparticles are either amorphous or nanocrystalline. Using this simple and inexpensive technique one can grow a TiOx layer on the surface of titanium electrodes or can synthetize nanocrystalline TiOx particles.
Link Between Metabolic Syndrome, Inflammation, and Eye Diseases
Authors: Pieńczykowska Kamila , Bryl Anna , Mrugacz Małgorzata
Published: February 2025

Metabolic syndrome (MetS)—a cluster of conditions including obesity, hypertension, dyslipidemia, and insulin resistance—is increasingly recognized as a key risk factor for the development of various eye diseases. The metabolic dysfunctions associated with this syndrome contribute to vascular and neurodegenerative damage within the eye, influencing disease onset and progression. Understanding these links highlights the importance of early diagnosis and management of metabolic syndrome to prevent vision loss and improve ocular health outcomes. This review explores the intricate interplay between metabolic syndrome, chronic low-grade inflammation, and eye diseases such as diabetic retinopathy, age-related macular degeneration, glaucoma, and dry eye syndrome. It highlights how inflammatory mediators, oxidative damage, and metabolic dysregulation converge to compromise ocular structures, including the retina, optic nerve, and ocular surface. We discuss the molecular and cellular mechanisms underpinning these associations and examine evidence from clinical and experimental studies. Given the rising global prevalence of metabolic syndrome, addressing this connection is crucial for improving overall patient outcomes and quality of life. Future research should focus on delineating the precise mechanisms linking these diseases as well as exploring targeted interventions that address both metabolic and ocular health.
Enhancing Passion Fruit Resilience: The Role of Hariman in Mitigating Viral Damage and Boosting Productivity in Organic Farming Systems
Authors: Santos-Jiménez José Leonardo , Montebianco Caroline de Barros , Bernardino Mariana Collodetti , Barreto-Bergter Eliana , Rosa Raul Castro Carriello , Vaslin Maite Freitas Silva
Published: February 2025

This study investigates the molecular mechanisms by which Hariman mitigates damage and productivity losses caused by Cucumber Aphid-Borne Mosaic Virus (CABMV) in the passion fruit genotypes ‘FB300’ and ‘H09-110/111’ under greenhouse and field conditions in Rio de Janeiro, Brazil. Hariman treatment induced the upregulation of key defense genes and phytohormones in response to CABMV infection, enabling treated plants to counteract virus-induced developmental impairments effectively. The relative accumulation of CABMV and disease severity were significantly reduced, with treated plants showing no decline in growth parameters such as height, leaf count, flower production, or fruit set. Over 18 months, total productivity increased by 65.7% and 114% for ‘FB300’ and by 44% and 80% for ‘H09-110/111’ after one and two applications of Hariman, respectively. Notably, infected plants treated with Hariman outperformed healthy plants grown under similar conditions, underscoring the biofertilizer’s dual role in promoting plant growth while enhancing resistance to biotic stressors. These findings indicate that Hariman stimulates robust growth and induces the expression of the defense-related genes PR-3, SOD, POD12, PAL, and LOX2 alongside the expression of the phytohormone-associated genes SAUR20 and GA2ox across different passion fruit genotypes. The adoption of these sustainable technologies holds significant potential for enhancing passion fruit productivity in the face of diseases that severely threaten this crop.
Novel Cell Models to Study Myelin and Microglia Interactions
Authors: Santacreu-Vilaseca Marta , Moreno-Magallon Judith , Juanes-Casado Alba , Gil-Sánchez Anna , González-Mingot Cristina , Torres Pascual , Brieva Luis
Published: February 2025

Multiple sclerosis (MS) is characterized by demyelination and neuroinflammation, with oxidative stress playing a pivotal role in lesion pathology. This study aimed to investigate the differential cellular responses to myelin debris under varying oxidative states. Myelin oxidation was induced using a Cu–peroxide system, confirmed by elevated TBARS levels and autofluorescence. BV-2 microglia viability remained unaffected by myelin exposure. However, oxidized myelin significantly altered oxidative stress markers, autophagy, and iron metabolism, as evidenced by changes in Sod2, Tfr1, p62, and P-Erk/Erk ratios. Morphological analyses revealed time- and dose-dependent differences in myelin processing, with oxidized myelin leading to distinct phagosome dynamics. Complementary studies using induced microglia-like cells (iMG)—a primary cell culture—confirmed the feasibility of employing oxidized microglia to study microglia activity. The use of iMGs provides a model closer to patient physiology, offering the potential to evaluate individual cellular responses to oxidative damage. This approach could be instrumental in identifying personalized therapeutic strategies by assessing patient-specific microglial behavior in response to myelin debris. These findings highlight the impact of myelin oxidative status on microglial function, advancing the understanding of oxidative stress in MS and paving the way for personalized medicine applications in neuroinflammation.
Hypothetical Pathogenetic Model of Membranous Nephropathy
Authors: Zdravkova Irina , Tilkiyan Eduard , Bozhkova Desislava , Kuskunov Teodor , Ronchev Yovko , Kirilov Boris
Published: February 2025

Membranous nephropathy (MN) is a disease with an etiology and pathogenesis that are still not fully understood, and it represents a great challenge. It is characterized by a variable course, spontaneous remissions and relapses. The inability to rely entirely on antibodies and the continuous threat of a malignant disease make the differentiation of MN types extremely difficult. Data of twelve patients with membranous nephropathy, ranging in age between 28 and 67 years, are presented; in total, seven men and five women were observed for a period of 2 to 10 years. In all patients, the diagnosis was confirmed through kidney biopsy and laboratory tests, including immunological, histopathological, and immunohistochemical tests. Histopathological and immunohistochemical tests were applied on available material from the thyroid gland in two patients and the gallbladder in two patients with MN. Data of 102 patients with MN and their comorbidities are evaluated in order to establish correlations. These and other data are used to build a hypothetical pathogenetic model that explains the etiology and the likely pattern of disease occurrence. We found a connection between chronic cholecystitis, thyroiditis, hepatitis, and other diseases in the occurrence of MN and disease course. From our practice and cases, we drew the conclusion that chronic inflammation in sites that express PLA2R leads to the formation of antibodies against PLA2R. These antibodies occur as a preformed immune complex or separately and are deposited in the subepithelial space, leading to MN appearance.
What Can We Learn from Dissecting Tortricid Females About the Efficacy of Mating Disruption Programs?
Authors: Knight Alan Lee , Preti Michele , Basoalto Esteban
Published: February 2025

Female mating success for the tortricids codling moth (CM), Cydia pomonella, Oriental fruit moth (OFM), Grapholita molesta, European grape vine moth (EGVM), Lobesia botrana, and five leafroller (LR) species under various mating disruption (MD) programs was reviewed at a time when new dual sex lures can provide alternative tools to assess female mating. Previous reliance on passive assessments such as tethering and virgin female-baited traps with laboratory moths are at odds with active trapping methods of wild moths. Additive factors such as delayed mating, adjustments in female behaviors, and greater levels of natural control may or may not contribute to the apparent success of MD. Current MD programs are not based solely on research, as economics and commercialization require some compromise. The complete sex pheromone blend is not always used. A delay in mating has been reported from the field with one study and suggested that reductions in fecundity would likely be minimal. There is no evidence that MD works better with low population densities. MD is an established technology, but the new dual sex lures are showing that the density of mated females is rather high. Efforts to improve the efficacy of MD are ongoing with a small cadre of researchers.
Reply to Moline et al. Comment on “Alliance for Sleep Clinical Practice Guideline on Switching or Deprescribing Hypnotic Medications for Insomnia. J. Clin. Med. 2023, 12, 2493”
Authors: Watson Nathaniel F. , Benca Ruth M. , Krystal Andrew D. , McCall William V. , Neubauer David N.
Published: February 2025

We thank Moline et al [...]
Similarity Index Values in Fuzzy Logic and the Support Vector Machine Method Applied to the Identification of Changes in Movement Patterns During Biceps-Curl Weight-Lifting Exercise
Authors: Peres André B. , Almeida Tiago A. F. , Massini Danilo A. , Macedo Anderson G. , Espada Mário C. , Robalo Ricardo A. M. , Oliveira Rafael , Brito João P. , Pessôa Filho Dalton M.
Published: February 2025

Background/Objectives: Correct supervision during the performance of resistance exercises is imperative to the correct execution of these exercises. This study presents a proposal for the use of Morisita–Horn similarity indices in modelling with machine learning methods to identify changes in positional sequence patterns during the biceps-curl weight-lifting exercise with a barbell. The models used are based on the fuzzy logic (FL) and support vector machine (SVM) methods. Methods: Ten male volunteers (age: 26 ± 4.9 years, height: 177 ± 8.0 cm, body weight: 86 ± 16 kg) performed a standing barbell bicep curl with additional weights. A smartphone was used to record their movements in the sagittal plane, providing information about joint positions and changes in the sequential position of the bar during each lifting attempt. Maximum absolute deviations of movement amplitudes were calculated for each execution. Results: A variance analysis revealed significant deviations (p < 0.002) in vertical displacement between the standard execution and execution with a load of 50% of the subject’s body weight. Experts with over thirty years of experience in resistance-exercise evaluation evaluated the exercises, and their results showed an agreement of over 70% with the results of the ANOVA. The similarity indices, absolute deviations, and expert evaluations were used for modelling in both the FL system and the SVM. The root mean square error and R-squared results for the FL system (R2 = 0.92, r = 0.96) were superior to those of the SVM (R2 = 0.81, r = 0.79). Conclusions: The use of FL in modelling emerges as a promising approach with which to support the assessment of movement patterns. Its applications range from automated detection of errors in exercise execution to enhancing motor performance in athletes.
Advancing Breast Cancer Care in Patients Aged 80 and Above: A Personalized and Multidisciplinary Management to Better Outcomes
Authors: Natale Maria , Di Leone Alba , Fusco Domenico , Accetta Cristina , Bellieni Andrea , Carnassale Beatrice , D’Archi Sabatino , De Lauretis Flavia , Di Guglielmo Enrico , Franco Antonio , Giannarelli Diana , Magno Stefano , Moschella Francesca , Sanchez Alejandro Martin , Scardina Lorenzo , Silenzi Marta , Masetti Riccardo , Franceschini Gianluca
Published: February 2025

Background: Breast cancer in women aged 80 years and older accounts for about 12% of cases, but its management is challenging due to the population’s heterogeneity and the lack of relevant evidence-based guidelines. Treatment decisions must consider biological age, comorbidities, life expectancy, therapy-related toxicities, and tumor biology. This study evaluates the clinical outcomes of elderly breast cancer patients treated with a multidisciplinary approach, including oncologists, surgeons, and geriatric specialists. Materials and Methods: A retrospective analysis of breast cancer patients aged ≥80 years treated at Fondazione Policlinico Universitario Agostino Gemelli IRCCS in Rome, Italy, from January 2016 to December 2020 was conducted. The study reviewed clinicopathological data, surgery, adjuvant therapies, and clinical outcomes. Treatment decisions were guided by multidisciplinary evaluations, including onco-geriatric assessments (GA) and guided treatment decisions. Primary outcomes included overall survival (OS), disease-free survival (DFS), and cancer-specific survival (CSS). Surgical and treatment-related complications were also documented. Results: A total of 238 patients aged ≥80 years were included in the study. Of these, 203 (85.3%) underwent breast-conserving surgery, while 35 (14.7%) underwent mastectomy. Axillary surgery was performed in 129 (54%) cases. Regarding adjuvant treatments, 93 (39.1%) patients received radiotherapy, and 101 (42.4%) received endocrine therapy alone. Chemotherapy was administered to six high-risk patients following GA, with no reported toxicities. Over a median follow-up of 42.3 months, the study reported one local recurrence (0.5%), one regional node recurrence (0.5%), and 19 cases of distant metastases (9%). A total of 19 patients (9%) died due to breast cancer. The overall complication rate was low, with 10% experiencing wound dehiscence, hematoma, lymphedema, or similar issues. Five-year survival outcomes were OS 73.3%, DFS 66.6%, and CSS 88.5%. Conclusions: This study highlights that a multidisciplinary approach to breast cancer management in patients aged ≥80 years yields favorable clinical outcomes with low recurrence, metastasis, and complication rates. The personalized treatment strategies, guided by onco-geriatric assessments, balance survival benefits with quality of life while minimizing risks of overtreatment or undertreatment. These findings emphasize the importance of individualized care in this complex patient population and offer valuable insights for optimizing management strategies as the elderly demographic continues to grow.
Executive Functions Training Improves Language Abilities in Aphasia Rehabilitation: A Systematic Review
Authors: Culicetto Laura , Latella Desirèe , Lo Buono Viviana , Orecchio Fabio , Murdaca Anna Maria , Quartarone Angelo , Marino Silvia
Published: February 2025

Background/Objectives: In recent years, the popularity of non-verbal cognitive training for aphasia has increased. Building on evidence that language abilities engage brain areas involved in executive functions (EFs) processing, this review aims to analyze the utility of EFs training alone or combined with traditional rehabilitation approaches to improve language abilities in aphasia. Methods: Systematic searches were performed in four databases evaluating studies focusing on the effects of EFs training in language rehabilitation, yielding 185 studies. After reading the full text of the selected studies and applying predefined inclusion criteria, nine studies were included based on pertinence and relevance to the topic. This systematic review has been registered in the Prospective Register of Systematic Reviews (PROSPERO 2024) with the number CRD42024519087. Results: The results of the analyzed studies indicate that various EFs training methods, such as computer-assisted executive control training, Cognitive Flexibility in Aphasia Therapy (CFAT), and the Dr. Neuronowski® program, as well as the combination of transcranial direct current stimulation (tDCS) with EFs training, can lead to improvements in language abilities in people with aphasia. Additionally, EFs training often results in specific effects on treated functions like working memory (near transfer effects) and untreated ones such as spoken sentence comprehension (far transfer effects). Conclusions: Despite the heterogeneity of the treatments and the small simple size of the studies analyzed, preliminary results are promising. Future research should further explore the effectiveness and specific contribution of EFs training to improving language functions in aphasia.
Spatial and Temporal Dynamics of Territorial Spatial Conflicts and Construction Land Expansion in Guizhou Province: A 40-Year Perspective
Authors: Wang Huaiyu , Yang Liu , Jiao Hongzan
Published: February 2025

Territorial spatial conflicts (TSCs) refer to a contradiction of utilization resulting from the inconsistency of the needs and objectives of different subjects of interest for spatial resources in planning, utilization, and management. This research aimed to unveil the TSCs, construction land expansion (CLE), and their relationship in Guizhou Province from 1980 to 2020, both temporally and spatially. This paper established indicators to assess CLE, including construction land expansion velocity, construction land expansion intensity, and construction land expansion pattern to analyze the expansion characteristics of construction land in Guizhou Province. At the same time, the territorial spatial conflict indicator (SCII) was constructed to study the TSCs in Guizhou Province, and its evolution pattern was explored through the cold hotspot analysis. On this basis, it investigated the relationship and linkage between TSCs and CLE through the ordinary least squares (OLS) regression model and geographically weighted (GWR) regression model. Furthermore, this paper also constructed an economic elasticity coefficient and a population elasticity coefficient to analyze the collaborative relationship between TSCs and GDP along with population volume. The research revealed that while the velocity and intensity of CLE in Guizhou Province have escalated over time, this expansion displayed considerable geographical variation across various locations. Simultaneously, the TSCs intensified, demonstrating a slight positive correlation with the expansion. The study of the spatial and temporal evolution characteristics and response relationship between the TSCs and CLE provided a reference for the optimization of regional territorial space. It is highly valuable and significant in fostering efficient utilization of land resources, adjusting to economic and social transformations, and improving the scientific rigor of spatial planning.
Study on the Spatial–Temporal Evolution and Driving Mechanisms of Tourism Ecological Security in the Jianmen Shu Road Heritage Area
Authors: Jiang Chenmingyang , Du Xinyu , Cai Jun , Li Hao , Peng Yi , Chen Qibing
Published: February 2025

Heritage is the commonwealth of all humankind. In the context of the rise in global tourism and the continuous deepening of cultural and tourism integration, tourism has emerged as an effective vehicle for the preservation and development of heritage sites. However, it also imposes adverse effects on the local ecological environment and heritage sites, exerting significant pressure on regional sustainable development. In this study, three cities along Jianmen Shu Road were selected as the study area. A comprehensive evaluation index system was developed for tourism ecological security (TES) based on the Driver–Pressure–State–Impact–Response model, and an in-depth analysis of its spatial–temporal evolution characteristics, spatial–temporal migration trends, and influencing factors was performed. The results show that (1) from 2012 to 2022, the average TES in the study area decreased annually, while it increased in Jiange County, Anzhou District, and Santai County. The TES indices were generally higher in areas with a high density of heritage sites or developed economies. Additionally, the districts and counties along the Jianmen Shu Road route never exhibited a deteriorated state. (2) From 2012 to 2022, TES in the study area exhibited an obvious “northeast–southwest” directional pattern, and its center of gravity followed a “V”-shaped trajectory. Overall, the spatial patterns showed minimal variation and exhibited agglomeration characteristics. (3) From 2012 to 2022, the main factors influencing TES included the density of Jianmen Shu Road heritage sites (S6), the number of 3A and above scenic areas (S5), the proportion of cultural tourism and sports in total expenditure (R3), the Normalized Difference Vegetation Index (NDVI) (S4), and other tourism and environmental factors. Moreover, TES systems are becoming increasingly complex and diverse. Finally, based on the results, a comprehensive conceptual framework of the driving mechanism was developed. Additionally, four targeted and scientifically grounded policy recommendations were formulated for restoring, protecting, and managing the TES in the Jianmen Shu Road Heritage Area. This study provides significant reference value for ecological environment preservation and the high-quality development of cultural tourism integration in heritage areas.
Avian Diversity and Habitat Preferences in Scrubland Ecosystems of the Eastern Ghats of Southern Odisha, India
Authors: Mahanta Gayatri , Panda Rajendra Mohan , Palita Sharat Kumar
Published: February 2025

Birds, potential pollinators and bio-indicators of ecosystem health, are important components of the global ecosystems. Habitat degradation and anthropogenic disturbance have influenced their survival. Here, we highlight the bird diversity and habitat preferences in scrublands in the Eastern Ghats by considering nine scrub sites. We documented 128 bird species, where Passeriformes were most abundant with 77 species. Shrub cover, herbaceous vegetation, exposed rock surfaces, bryophytes vegetation, and soil characteristics were studied to assess habitat preferences using analysis of variance, cluster analysis, and redundancy analysis. The studies on nine selected sites revealed reduced bird diversity in places of high anthropogenic disturbances and interrupted foraging. Argya strita, Cinnyris asiaticus, Merops orientalis, Picnonotus cafer, and Saxicola caprata showed close association with shrubs with ample foraging opportunities and nesting sites; Gracopica contra and Picnonotus jocosus showed preferences for herbs. C. asiaticus was not found to be associated with any vegetation. While herbs and shrubs have a strong and positive correlation, bryophytes and rocks exhibited strong but negative relationships. Scrubland sites with greater vegetation diversity and structural complexity supported more birds, indicating the significance of habitat characteristics in bird’s distribution. The present study at Koraput provides a basis for site-specific conservation to preserve the avian biodiversity in the Eastern Ghats of India. It also highlights the need for long-term monitoring and adaptive management to address environmental changes and ensure the preservation of these ecosystems.
Trigeminal Neuralgia Treatment via Piezosurgical Enlargement of the Mental Foramen
Authors: Jadach Radosław , Osypko Karolina
Published: February 2025

Background: This article and the novel surgical approach described here were inspired by the ideas and observations of the late professors T. Pawela and J. Wnukiewicz. The authors present the medical history and unique surgical treatment of four patients with trigeminal neuralgia, who, despite pharmacological treatment and numerous specialists being involved in the treatment process, continued suffering. Our belief is that the direct cause of the symptoms is a narrow mental foramen, which compresses the mental nerve. It can be easily verified by local anesthesia administration to verify the trigger point, and by analyzing CBCT scans with a special emphasis on the diameter of both mental foramina. Methods: Surgical decompression by narrow mental foramen enlargement was conducted with a piezosurgical device. In this procedure, a rectangle of cortical bone is gently and precisely cut around the mental foramen and then into smaller pieces. This technique enables its easy and safe removal. Then, the mental nerve is left loose, uncompressed. Results: All four patients reported immediate recovery, their pain attacks stopped, and their quality of life improved significantly. One patient reported temporal hypoesthesia that lasted 5 months post-op. About 2 years post-op, another patient reported rare recurrences of pain, although much less severe than before surgery. Conclusions: This type of treatment may be considered when trigeminal neuralgia cannot be classified as classic or as secondary and is unresponsive to pharmacological treatment. A piezosurgical device seems to be the safest option in terms of potential damage to the nerve. Further research should include a larger sample of patients and focus on analyzing the mental foramina diameter of patients with idiopathic trigeminal neuralgia.