bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Assessment of Nitrogen Concentration Effect on Microalgal Growth and Biochemical Characteristics of Two Chlorella Strains Cultivated in Digestate
Authors: Mastropetros Savvas Giannis , Koutra Eleni , Amouri Mohammed , Aziza Majda , Ali Sameh Samir , Kornaros Michael
Published: June 2022

Microalgae have been recently recognized as a promising alternative for the effective treatment of anaerobic digestion effluents. However, to date, a widely applied microalgae-based process is still absent, due to several constraints mainly attributed to high ammonia concentrations and turbidity, both hindering microalgal growth. Within this scope, the purpose of the present study was to investigate the performance of two Chlorella strains, SAG 211-11b and a local Algerian isolate, under different nitrogen levels, upon ammonia stripping. The experiments were performed on cylindrical photobioreactors under controlled pH (7.8 ± 0.2) and temperature (25 ± 2 °C). Cultures were monitored for biomass production and substrate consumption. After sampling at the beginning of the stationary phase of growth (12th day) and after the maturation of the cells (24th day), an analysis of the produced biomass was conducted, in terms of its biochemical components. The local isolate grew better than C. vulgaris 211-11b, resulting in 1.43 mg L−1 biomass compared to 1.02 mg L−1 under 25 mg NH4-N L−1, while organic carbon and nutrient consumption varied between the two strains and different conditions. Concerning biomass quality, a high initial NH4-N concentration led to high protein content, while low nitrogen levels favored fatty acid (FA) accumulation, though the production of pigments was inhibited. In particular, the protein content of the final biomass was determined close to 45% of the dry weight in all experimental scenarios with adequate nitrogen, while proteins decreased, and the fatty acids approached 20% in the case of the local isolate grown on the substrate with the lowest initial ammonium nitrogen (25 mg NH4-N L−1). The novelty of the present work lies in the comparison of a microalga with industrial applications against a local isolate of the same species, which may prove to be even more robust and profitable.
Dual-Phase Mixed Protonic-Electronic Conducting Hydrogen Separation Membranes: A Review
Authors: Cheng Hongda
Published: June 2022

Owing to the excellent properties of high selectivity, high thermal stability, and low cost, in the past twenty years, mixed protonic-electronic conducting hydrogen separation membranes have received extensive attention. In particular, dual-phase mixed protonic-electronic conducting membranes with high ambipolar conductivity are more attractive because of the high hydrogen permeability. This paper aimed to present a review of research activities on the dual-phase membranes, in which the components, the characteristics, and the performances of different dual-phase membranes are introduced. The key issues that affect the membrane performance such as the elimination of the inter-phase reaction, the combination mode of the phases, the phase ratio, and the membrane configuration were discussed. The current problems and future trends were simply recommended.
Modeling Receptor Motility along Advecting Lipid Membranes
Authors: Arricca Matteo , Salvadori Alberto , Bonanno Claudia , Serpelloni Mattia
Published: June 2022

This work aims to overview multiphysics mechanobiological computational models for receptor dynamics along advecting cell membranes. Continuum and statistical models of receptor motility are the two main modeling methodologies identified in reviewing the state of the art. Within the former modeling class, a further subdivision based on different biological purposes and processes of proteins’ motion is recognized; cell adhesion, cell contractility, endocytosis, and receptor relocations on advecting membranes are the most relevant biological processes identified in which receptor motility is pivotal. Numerical and/or experimental methods and approaches are highlighted in the exposure of the reviewed works provided by the literature, pertinent to the topic of the present manuscript. With a main focus on the continuum models of receptor motility, we discuss appropriate multiphyisics laws to model the mass flux of receptor proteins in the reproduction of receptor relocation and recruitment along cell membranes to describe receptor–ligand chemical interactions, and the cell’s structural response. The mass flux of receptor modeling is further supported by a discussion on the methodology utilized to evaluate the protein diffusion coefficient developed over the years.
Novel Thin Film Nanocomposite Membranes Based on Chitosan Succinate Modified with Fe-BTC for Enhanced Pervaporation Dehydration of Isopropanol
Authors: Burts Katsiaryna , Plisko Tatiana , Dmitrenko Mariia , Zolotarev Andrey , Kuzminova Anna , Bildyukevich Alexandr , Ermakov Sergey , Penkova Anastasia
Published: June 2022

The application of environmentally friendly and energy-efficient membrane processes allows improvement the ecological safety and sustainability of industrial production. However, the effective application of membrane processes requires novel high-performance thin film composite (TFC) membranes based on biopolymers to solve environmental problems. In this work for the first time novel thin film nanocomposite (TFN) membranes based on biopolymer chitosan succinate (ChS) modified with the metal organic framework iron 1,3,5-benzenetricarboxylate (Fe-BTC) were developed for enhanced pervaporation dehydration. The formation of a selective layer of TFN membranes on the porous membrane-support was carried out by two methods—dynamic technique and physical adsorption. The effect of the membrane formation method and Fe-BTC content in ChS layer on the structure and physicochemical properties of TFN membranes was investigated. The developed TFN ChS-based membranes were evaluated in the pervaporation dehydration of isopropanol (12–30 wt.% water). It was found that TFN ChS-Fe-BTC membranes prepared by two methods demonstrated improved permeation flux compared to the reference TFC ChS membrane. The best transport properties in pervaporation dehydration of isopropanol (12–30 wt.% water) were possessed by TFN membranes with 40 wt.% Fe-BTC prepared by dynamic technique (permeation flux 99–499 g m−2 h−1 and 99.99% water in permeate) and TFN membranes with 5 wt.% Fe-BTC developed by physical adsorption (permeation flux 180–701 g m−2 h−1 and 99.99% water in permeate).
Tuning the Morphology and Gas Separation Properties of Polysulfone Membranes
Authors: Kluge Steven , Kose Tillmann , Tutuş Murat
Published: June 2022

The present work deals with the modification of casting solutions for polysulfone gas separation membranes fabricated by wet-phase inversion. The aim was to fabricate membranes with thin gas separation layers below one micrometer of thickness and a sponge-like support structure. With decreasing thicknesses of the separation layers, increasing permselectivities were observed. For the first time, we could show that permeabilities and diffusion coefficients of certain gases are orders of magnitude lower in separation layers of membranes below 500 Å of thickness compared to separation layers with a thickness above 1 micrometer. These results indicate that the selection of the solvent system has a huge impact on the membrane properties and that the permeability and diffusion coefficient are not material-related properties. Thus, they cannot be applied as specific indicators for gas-separating polymers. In this publication, scanning electron microscopy and gas permeation measurements were carried out to prove the gas separation properties and morphologies of polysulfone membranes.
Effect of Wall Thickness and Surface Conditions on Creep Behavior of a Single-Crystal Ni-Based Superalloy
Authors: Körber Selina , Wolff-Goodrich Silas , Völkl Rainer , Glatzel Uwe
Published: June 2022

The influence of wall thickness and specimen surface on the creep behavior of the single-crystal nickel-based superalloy MAR M247LC is studied. Specimens with wall thicknesses of 0.4, 0.8, 1 and 2 mm, with and without casting surface, are compared to specimens of the same wall thickness prepared from bulk material. Creep behavior turned out to be independent from surface conditions even for the thinnest specimens. The thickness debit effect is not pronounced for short creep rupture times (≤100 h at 980 °C), whereas it is significant for creep rupture times longer than ~200 h at 980 °C. The thickness debit effect is time-dependent and caused by oxidation and diffusion-controlled mechanisms.
Mechanical Behavior of Multi-Material Single-Lap Joints under High Rates of Loading Using a Split Hopkinson Tension Bar
Authors: Rüthnick Pascal , Ledford Noah , Imbert Mathieu , May Michael
Published: June 2022

In the presented research, a split Hopkinson tension bar (SHTB) was used to measure the mechanical response of multi-material single-lap joints in the high-rate loading regime. High-performance applications require high-quality measurements of the mechanical properties to define safe design rules. Servo-hydraulic machines are commonly used to investigate such small structures, but they are prone to produce oscillation-affected force measurements. To improve force–displacement measurements, an SHTB was chosen to investigate these joints. Three different kinds of joints were tested: multi-material bolted joints, multi-material bonded joints, and multi-material bonded/bolted joints. One substrate of the joints was made of aluminum (Al-2024-T3) and the other one was made of a laminated composite (TC250). A countersunk titanium bolt and a crash-optimized epoxy adhesive (Betamate 1496 V) were used to fasten the joints. A constant impedance mounting device was implemented to limit wave reflections and to improve the signal quality. Quasi-static experiments at a servo-hydraulic machine were performed to compare the data with the respective data from the high-rate loading conditions. The presented research shows that high-quality high-rate tests of multi-material single-lap joints can be achieved by employing an SHTB. With this high-quality measurement, a rate dependency of the mechanical behavior of these joints was identified. The dynamic increase (DI), which is the ratio of a high rate of loading over quasi-static loading, was measured for each of the joint types, where the dynamic increase in the max force was DI = 1.1 for the bolted, DI = 1.4 for the bonded, and DI = 1.6 for the bonded/bolted joints.
Microstructure Change and Mechanism during the Metal Machining Process, Modeling, and Applications
Authors: Feng Yixuan , Zhao Man
Published: June 2022

It is critical to understand the fundamental mechanisms during the metal-machining process [...]
Damage Evolution Simulations via a Coupled Crystal Plasticity and Cohesive Zone Model for Additively Manufactured Austenitic SS 316L DED Components
Authors: Darabi Roya , Azinpour Erfan , Ferreira Andre , de Sa Jose Cesar , Reis Ana , Dzugan Jan
Published: June 2022

This study presents a microstructural model applicable to additively manufactured (AM) austenitic SS 316L components fabricated via a direct energy deposition (DED) process. The model is primarily intended to give an understanding of the effect of microscale and mesoscale features, such as grains and melt pool sizes, on the mechanical properties of manufactured components. Based on experimental observations, initial assumptions for the numerical model regarding grain size and melt pool dimensions were considered. Experimental observations based on miniature-sized 316L stainless steel DED-fabricated samples were carried out to shed light on the deformation mechanism of FCC materials at the grain scale. Furthermore, the dependency of latent strain hardening parameters based on the Bassani–Wu hardening model for a single crystal scale is investigated, where the Voronoi tessellation method and probability theory are utilized for the definition of the grain distribution. A hierarchical polycrystalline modeling methodology based on a representative volume element (RVE) with the realistic impact of grain boundaries was adopted for fracture assessment of the AM parts. To qualify the validity of process–structure–property relationships, cohesive zone damage surfaces were used between melt pool boundaries as the predefined initial cracks and the performance of the model is validated based on the experimental observations.
TraVis Pies: A Guide for Stable Isotope Metabolomics Interpretation Using an Intuitive Visualization
Authors: De Craemer Sam , Driesen Karen , Ghesquière Bart
Published: June 2022

Tracer metabolomics is a powerful technology for the biomedical community to study and understand disease-inflicted metabolic mechanisms. However, the interpretation of tracer metabolomics results is highly technical, as the metabolites’ abundances, tracer incorporation and positions on the metabolic map all must be jointly interpreted. The field is currently lacking a structured approach to help less experienced researchers start the interpretation of tracer metabolomics datasets. We propose an approach using an intuitive visualization concept aided by a novel open-source tool, and provide guidelines on how researchers can apply the approach and the visualization tool to their own datasets. Using a showcase experiment, we demonstrate that the visualization approach leads to an intuitive interpretation that can ease researchers into understanding their tracer metabolomics data.
Investigation into Mode Localization of Electrostatically Coupled Shallow Microbeams for Potential Sensing Applications
Authors: Alneamy Ayman M. , Ouakad Hassen M.
Published: June 2022

With the constant need for the development of smart devices, Micro-Electro-Mechanical Systems (MEMS) based smart sensors have been developed to detect hazard materials, micro-particles or even toxic substances. Identifying small particles using such micro-engineering technology requires designing sensors with high sensitivity, selectivity and ease of integration with other electronic components. Nevertheless, the available detection mechanism designs are still juvenile and need more innovative ideas to be even more competitive. Therefore, this work aims to introduce a novel, smart and innovative micro-sensor design consisting of two weakly electrostatically coupled microbeams (both serving as sensors) and electrically excited using a stationary electrode assuming a dc/ac electric signal. The sensor design can be tuned from straight to eventually initially curved microbeams. Such an arrangement would develop certain nonlinear phenomena, such as the snap-through motion. This behavior would portray certain mode veering/mode crossing and ultimately mode localization and it would certainly lead in increasing the sensitivity of the mode-localized based sensing mechanism. These can be achieved by tracking the change in the resonance frequencies of the two microbeams as the coupling control parameter is varied. To this extent, a nonlinear model of the design is presented, and then a reduced-order model considering all geometric and electrical nonlinearities is established. A Long-Time Integration (LTI) method is utilized to solve the static and dynamics of the coupled resonators under primary lower-order and higher-order resonances, respectively. It is shown that the system can display veering and mode coupling in the vicinity of the primary resonances of both beams. Such detected modal interactions lead to an increase in the sensitivity of the sensor design. In addition, the use of two different beam’s configurations in one device uncovered a possibility of using this design in detecting two potential substances at the same time using the two interacting resonant peaks.
A Magnetic Metal Hard Mask on Silicon Substrate for Direct Patterning Ultra-High-Resolution OLED Displays
Authors: Chen Lin , Wang Xiuxia , Wei Yu , Zhou Chenggang
Published: June 2022

With the development of virtual reality/augmented reality (VR/AR) display devices, the conventional fine metal mask is limited by the wet etch process, which no longer meets the demand for high pixels per inch (PPI) displays. We deposited a layer of magnetic metal on the silicon substrate by physical vapor deposition (PVD), and then developed a 2-inch, 3175 PPI magnetic metal hard mask on silicon substrate (MMS) through deep silicon etching and other micro-nano processing for patterning Organic Light-Emitting Diodes (OLED) displays, which can achieve smaller pixel size and higher PPI. MMS can not only solve the bottleneck problem of the traditional invar alloy shadow mask with low PPI, but also reduce the bending caused by the deformation of the silicon-based mask due to gravity, so that it achieves high PPI and higher uniformity in OLED displays.
Wide Spectrum Potent Antimicrobial Efficacy of Wound Dressings Impregnated with Cuprous Oxide Microparticles
Authors: Borkow Gadi , Roth Tohar , Kalinkovich Alexander
Published: June 2022

Copper has intrinsic antimicrobial properties. Wound dressings impregnated with cuprous oxide microparticles (hereafter termed COD) have been cleared for the management of acute and chronic wounds by the FDA and other regulatory bodies. The COD reduced the viable microbial titers of a wide spectrum of microbes by more than 10,000-fold (4-logs) within 3 h of exposure at 37 °C (p < 0.001). Similar microbial titer reductions were achieved by 3-year naturally aged COD dressings, showing the stability of the biocidal efficacy over time. The potent biocidal efficacy of the COD was maintained even after 7 daily consecutive inoculations of the dressings with ~106 CFU. COD with an adhesive contour blocked the passage of bacteria from the exterior environment to the wound bed side of the dressing even after 7 daily consecutive inoculations of different bacteria on the outer surface of the dressings. Taken together, the study demonstrates the wide spectrum potent in vitro biocidal efficacy of the cuprous oxide impregnated dressings against a wide panel of microorganisms.
Microbiological Aspects of Unique, Rare, and Unusual Fatty Acids Derived from Natural Amides and Their Pharmacological Profile
Authors: Dembitsky Valery M.
Published: June 2022

In the proposed review, the pharmacological profile of unique, rare, and unusual fatty acids derived from natural amides is considered. These amides are produced by various microorganisms, lichens, and fungi. The biological activity of some natural fatty acid amides has been determined by their isolation from natural sources, but the biological activity of fatty acids has not been practically studied. According to QSAR data, the biological activity of fatty acids is shown, which demonstrated strong antifungal, antibacterial, antiviral, antineoplastic, anti-inflammatory activities. Moreover, some fatty acids have shown rare activities such as antidiabetic, anti-infective, anti-eczematic, antimutagenic, and anti-psoriatic activities. For some fatty acids that have pronounced biological properties, 3D graphs are shown that show a graphical representation of unique activities. These data are undoubtedly of both theoretical and practical interest for chemists, pharmacologists, as well as for the pharmaceutical industry, which is engaged in the synthesis of biologically active drugs.
Extracellular DNA: A Critical Aspect of Marine Biofilms
Authors: Tuck Benjamin , Salgar-Chaparro Silvia J. , Watkin Elizabeth , Somers Anthony , Forsyth Maria , Machuca Laura L.
Published: June 2022

Multispecies biofilms represent a pervasive threat to marine-based industry, resulting in USD billions in annual losses through biofouling and microbiologically influenced corrosion (MIC). Biocides, the primary line of defence against marine biofilms, now face efficacy and toxicity challenges as chemical tolerance by microorganisms increases. A lack of fundamental understanding of species and EPS composition in marine biofilms remains a bottleneck for the development of effective, target-specific biocides with lower environmental impact. In the present study, marine biofilms are developed on steel with three bacterial isolates to evaluate the composition of the EPSs (extracellular polymeric substances) and population dynamics. Confocal laser scanning microscopy, scanning electron microscopy, and fluorimetry revealed that extracellular DNA (eDNA) was a critical structural component of the biofilms. Parallel population analysis indicated that all three strains were active members of the biofilm community. However, eDNA composition did not correlate with strain abundance or activity. The results of the EPS composition analysis and population analysis reveal that biofilms in marine conditions can be stable, well-defined communities, with enabling populations that shape the EPSs. Under marine conditions, eDNA is a critical EPS component of the biofilm and represents a promising target for the enhancement of biocide specificity against these populations.
Adsorption and Desorption of Coal Gangue toward Available Phosphorus through Calcium-Modification with Different pH
Authors: Ye Tiantian , Min Xiangyu , Jiang Xuzi , Sun Mingyue , Li Xinju
Published: June 2022

The chemical reaction between calcium ions (Ca2+) and phosphate in the soil is the main way to maintain the availability of soil phosphorus. Thus, we believe stimulating coal gangue with Ca2+ solution would be an effective way to improve its adsorption and desorption capacity toward phosphate. In order to explore the effects of different pH of Ca2+ solution on the modified effect of coal gangue, we conducted mechanical grinding (<1 mm), high temperature calcination (800 °C), and the stimulation of Ca2+ solution with different pH (2, 7, 13), to prepare acidic calcium-modified coal gangue (Ac-CG) (Ac-CG, acidic calcium-modified coal gangue; Ne-CG, neutral calcium-modified coal gangue; Al-CG, alkali calcium-modified coal gangue; RCG, raw coal gangue), neutral calcium-modified coal gangue (Ne-CG), and alkali calcium-modified coal gangue (Al-CG); raw coal gangue (RCG) was regarded as the control. The results indicated that Al-CG had better phosphate adsorption (3.599 mg g−1); this favorable adsorption performance of Al-CG was related to the formation of hydrated calcium silicate gel and ettringite, which provided more Ca2+, Al3+, and hydroxyl groups, and a larger specific surface area (9.497 m2 g−1). Moreover, Al-CG not only held more phosphate but also maintained its availability longer for plants. It is suggested that stimulating coal gangue with Ca2+ solution under alkaline conditions is a perfect way to enhance its adsorption and desorption capacity toward phosphate; the Al-CG we prepared could be used as filling material and soil conditioner in the reclamation area.
Cryogenic Soil—Product of Mineral Weathering Processes
Authors: Zhang Ze , Zhai Jinbang , Melnikov Andrey , Zhang Shengrong , Li Xianglong
Published: June 2022

Since the Quaternary, the alternate climate of dry and wet, cold and warm, and the emergence of glacial and interglacial periods have led to great changes in the global environment and climate. As an event closely related to cold climate, cryogenic soil has important reference significance for the study of climate change in a certain region and time period. The research on cryogenic soils mainly focuses on the following three aspects: particle size composition, surface morphology and mineral composition. Through the study of the relevant literature, we find that the correlation coefficient of particle size composition before and after freeze-thaw is used to determine the cause of cryogenic weathering. Due to the singleness of judgment conditions, the result is difficult to be convincing; It is difficult to prove the microscopic morphology of the cause of cryogenic weathering from a single mineral of quartz. Therefore, it is necessary to start with more types of primary minerals, and analyze the differences in the particle shape and microscopic surface morphology of different types of primary minerals during the cryogenic weathering process. And on this basis, the typical mineral morphology of the cause of cryogenic weathering is comprehensively judged; Freeze-thaw has little effect on the mineral composition of the soil, but has a greater impact on the size of the mineral particles, and this size change corresponds to the phenomenon of particles silt-fication. The mineral composition also controls the geochemical composition, and the insignificance of the mineral-chemical composition in the process of cryogenic silt-fication increases the difficulty of judging the cause of cryogenic weathering.
Prospective Evaluation of Geothermal Resources in the Shangqiu Uplift of the Southern North China Basin with Magnetotelluric Detection
Authors: Xu Bowen , Zhu Huailiang , Liu Zhilong , Shao Bingsong , Ye Gaofeng
Published: June 2022

The magnetotelluric sounding (MT) method is used to detect and study the deep stratigraphic structure and hidden faults in the Shangqiu Uplift. A total of 4 MT profiles are arranged, and 97 stations are collected. The nonlinear conjugate gradient (NLCG) two-dimensional inversion method is used to jointly invert data from both the TE and TM modes after a dimensionality analysis and impedance tensor decomposition; reliable two-dimensional resistivity models are produced since the data quality is excellent. Three-dimensional inversion is carried out to full impedance tensor as well to produce a three-dimensional resistivity model of the study area, which shows good consistency with the two-dimensional models. The results show that the electrical structure of the Shangqiu Uplift has typical layered characteristics, which can be divided into three layers from top to bottom, namely low-, medium-to-high-, and high-resistivity layers. According to the resistivity models and in combination with the gravity, aeromagnetic, seismic, and regional geological data of the study area, a geological map of the basement rock of the Shangqiu Uplift is produced. Two prospective geothermal anomaly areas are proposed according to the distribution of the high-resistivity anomaly formed by the basement uplift, which has a good corresponding relationship with the high-value area of the regional geothermal field. A geothermal exploration well (SR-1) is constructed in one of the inferred prospective geothermal anomaly areas. The well is 1702 m deep, with a water output of 1500 m3/day and a wellhead water temperature of 51.5 °C. This is the geothermal well with the largest water yield in the Shangqiu area at present, which provides a new basis for future geothermal exploration, development, and utilization.
Quantification of Small-Scale Heterogeneity at the Core–Mantle Boundary Using Sample Entropy of SKS and SPdKS Synthetic Waveforms
Authors: Pachhai Surya , Thorne Michael S. , Nissen-Meyer Tarje
Published: June 2022

Qualitative and quantitative analysis of seismic waveforms sensitive to the core–mantle boundary (CMB) region reveal the presence of ultralow-velocity zones (ULVZs) that have a strong decrease in compressional (P) and shear (S) wave velocity, and an increase in density within thin structures. However, understanding their physical origin and relation to the other large-scale structures in the lowermost mantle are limited due to an incomplete mapping of ULVZs at the CMB. The SKS and SPdKS seismic waveforms is routinely used to infer ULVZ presence, but has thus far only been used in a limited epicentral distance range. As the SKS/SPdKS wavefield interacts with a ULVZ it generates additional seismic arrivals, thus increasing the complexity of the recorded wavefield. Here, we explore utilization of the multi-scale sample entropy method to search for ULVZ structures. We investigate the feasibility of this approach through analysis of synthetic seismograms computed for PREM, 1-, 2.5-, and 3-D ULVZs as well as heterogeneous structures with a strong increase in velocity in the lowermost mantle in 1- and 2.5-D. We find that the sample entropy technique may be useful across a wide range of epicentral distances from 100° to 130°. Such an analysis, when applied to real waveforms, could provide coverage of roughly 85% by surface area of the CMB.
Behavior and Mechanism of a Novel Hydrophobic Collector in the Flotation of Bastnaesite
Authors: Yao Xiang , Yu Xinyang , Zeng Yuhui , Mao Linghan , Xie Honghui , Liu Shanming , He Guichun , Huang Zhiqiang , Wang Haolin , Liu Zhilin
Published: June 2022

In order to improve the recovery of rare earth elements, finding a collector with a strong selectivity ability had become the focus of research. In this paper, phenylpropyl hydroxamic acid (PHA) was used as a new hydrophobic surfactant collector for the separation of bastnaesite from calcite, and salicylic hydroxamic acid (SHA) was used as a reference collector. The results of a single mineral flotation test with SHA show that the reagent has good collection performance and selectivity. In addition, Zeta potential measurements and FTIR analysis show that PHA is adsorbed on the surface of bastnaesite by chemical adsorption, and the surface state of bastnaesite changes after PHA treatment. By XPS analysis, PHA interacts with Ce, and forms a Ce–O bond with Ce. It is speculated that the hydroxamic acid forms a five-element-chelated hydroxamic group with Ce on bastnaesite surface, so as to improve the hydrophobicity of bastnaesite, and make bastnaesite float more easily out of the pulp. According to DFT calculation, PHA has better adsorption capacity and stronger hydrophobicity than SHA, and shows superior electronic group capacity and chemical reactions that promote its flotation performance.
Rifaximin Protects against Malathion-Induced Rat Testicular Toxicity: A Possible Clue on Modulating Gut Microbiome and Inhibition of Oxidative Stress by Mitophagy
Authors: Omar Nesreen Nabil , Mosbah Rasha A. , Sarawi Wedad S. , Rashed Marwa Medhet , Badr Amira M.
Published: June 2022

Testicular dysfunction is caused by chronic exposure to environmental pollution, such as malathion, which causes oxidative stress, promoting cell damage. Autophagy is a key cellular process for eliminating malfunctioning organelles, such as the mitochondria (mitophagy), an eminent source of reactive oxygen species (ROS). Autophagy is crucial for protection against testicular damage. Rifaximin (RFX) is a non-absorbable antibiotic that can reshape the gut microbiome, making it effective in different gastrointestinal disorders. Interestingly, the gut microbiome produces short chain fatty acids (SCFAs) in the circulation, which act as signal molecules to regulate the autophagy. In this study, we investigated the regulatory effects of RFX on gut microbiota and its circulating metabolites SCFA and linked them with the autophagy in testicular tissues in response to malathion administration. Moreover, we divided the groups of rats that used malathion and RFX into a two-week group to investigate the mitophagy process and a four-week group to study mitochondriogenesis. The current study revealed that after two weeks of cotreatment with RFX, apoptosis was inhibited, oxidative stress was improved, and autophagy was induced. More specifically, PINK1 was overexpressed, identifying mitophagy activation. After four weeks of cotreatment with RFX, there was an increase in acetate and propionate-producing microflora, as well as the circulating levels of SCFAs. In accordance with this, the expression of PGC-1α, a downstream to SCFAs action on their receptors, was activated. PGC-1α is an upstream activator of mitophagy and mitochondriogenesis. In this sense, the protein expression of TFAM, which regulates the mitochondrial genome, was upregulated along with a significant decrease in apoptosis and oxidative stress. Conclusion: we found that RFX has a positive regulatory effect on mitophagy and mitochondria biogenesis, which could explain the novel role played by RFX in preventing the adverse effects of malathion on testicular tissue.
Cancer and Traditional Plant Knowledge, an Interesting Field to Explore: Data from the Catalan Linguistic Area
Authors: Gras Airy , Parada Montse , Pellicer Jaume , Vallès Joan , Garnatje Teresa
Published: June 2022

Cancer is the second cause of death in the world and is foreseen to be responsible for about 16 million deaths in 2040. Approximately, 60% of the drugs used to treat cancer are of natural origin. Besides the extensive use of some of these drugs in therapies, such as those derived from the genus Taxus, a significant number of plants have revealed themselves as useful against cancer in recent years. The field of ethnobotany focuses on documenting traditional knowledge associated with plants, constituting a starting point to uncover the potential of new plant-based drugs to treat or prevent, in this case, tumour diseases and side effects of chemotherapy and radiotherapy. From a series of extensive ethnobotanical prospections across the Catalan linguistic area (CLA), we have recorded uses for 41 taxa with antitumour effects. The two most quoted botanical families are Asteraceae and Ranunculaceae, and the most frequently reported species is Ranunculus parnassifolius, a high-mountain species, which is widely collected for this purpose. The reported species have been used to treat an important number of cancer types, focusing on preventive, palliative, and curative uses, as well as to deal with the side effects of conventional treatments. Comparing our results in CLA with previous data available in the most comprehensive databases of pharmacology and a review of cytotoxicity assays revealed that for the several species reported here, there was no previous evidence of traditional uses against cancer. Despite the need for further analyses to experimentally validate the information presented here, combining traditional uses and phylogenetically-informed strategies to phytochemical and pharmacological research would represent new avenues to establish more integrative approaches, hence improving the ability to select new candidate taxa in cancer research.
Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease
Authors: Zhang You-Gang , Liu Xia-Xia , Zong Jian-Cheng , Zhang Yang-Teng-Jiao , Dong Rong , Wang Na , Ma Zhi-Hui , Li Li , Wang Shang-Long , Mu Yan-Ling , Wang Song-Song , Liu Zi-Min , Han Li-Wen
Published: June 2022

Phytotherapy offers obvious advantages in the intervention of Coronary Artery Disease (CAD), but it is difficult to clarify the working mechanisms of the medicinal materials it uses. DGS is a natural vasoprotective combination that was screened out in our previous research, yet its potential components and mechanisms are unknown. Therefore, in this study, HPLC-MS and network pharmacology were employed to identify the active components and key signaling pathways of DGS. Transgenic zebrafish and HUVECs cell assays were used to evaluate the effectiveness of DGS. A total of 37 potentially active compounds were identified that interacted with 112 potential targets of CAD. Furthermore, PI3K-Akt, MAPK, relaxin, VEGF, and other signal pathways were determined to be the most promising DGS-mediated pathways. NO kit, ELISA, and Western blot results showed that DGS significantly promoted NO and VEGFA secretion via the upregulation of VEGFR2 expression and the phosphorylation of Akt, Erk1/2, and eNOS to cause angiogenesis and vasodilation. The result of dynamics molecular docking indicated that Salvianolic acid C may be a key active component of DGS in the treatment of CAD. In conclusion, this study has shed light on the network molecular mechanism of DGS for the intervention of CAD using a network pharmacology-driven strategy for the first time to aid in the intervention of CAD.
Connarus semidecandrus Jack Exerts Anti-Alopecia Effects by Targeting 5α-Reductase Activity and an Intrinsic Apoptotic Pathway
Authors: Jang Won Young , Kim Dong Seon , Park Sang Hee , Yoon Ji Hye , Shin Chae Yun , Huang Lei , Nang Ket , Kry Masphal , Byun Hye-Woo , Lee Byoung-Hee , Lee Sarah , Lee Jongsung , Cho Jae Youl
Published: June 2022

There is a growing demand for hair loss treatments with minimal side effects and recurrence potential. Connarus semidecandrus Jack has been used as a folk medicine for fever in tropical regions, but its anti-alopecia effects remain unclear. In this study, the anti-androgenic alopecia effect of an ethanol extract of Connarus semidecandrus Jack (Cs-EE) was demonstrated in a testosterone-induced androgenic alopecia (AGA) model, in terms of the hair–skin ratio, hair type frequency, and hair thickness. The area of restored hair growth and thickened hair population after Cs-EE treatment showed the hair-growth-promoting effect of Cs-EE. Histological data support the possibility that Cs-EE could reduce hair loss and upregulate hair proliferation in mouse skin by shifting hair follicles from the catagen phase to the anagen phase. Western blotting indicated that Cs-EE reduced the expression of the androgenic receptor. Cs-EE treatment also inhibited programmed cell death by upregulating Bcl-2 expression at the mRNA and protein levels. The anti-alopecia effect of Cs-EE was confirmed by in vitro experiments showing that Cs-EE had suppressive effects on 5-α reductase activity and lymph node carcinoma of the prostate proliferation, and a proliferative effect on human hair-follicle dermal papilla (HDP) cells. Apoptotic pathways in HDP cells were downregulated by Cs-EE treatment. Thus, Cs-EE could be a potential treatment for AGA.
Simultaneous Determination of Six Immunosuppressants in Human Whole Blood by HPLC-MS/MS Using a Modified QuEChERS Method
Authors: Zheng Min , Song Jianshi , Xue Hua , Li Hui , Lian Kaoqi
Published: June 2022

A high-performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of mycophenolic acid, mycophenolate mofetil, tacrolimus, rapamycin, everolimus and pimecrolimus in human whole blood by optimizing the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) preparation method. Whole blood was extracted into ethyl acetate, salted out with anhydrous magnesium sulfate, and purified with ethylenediamine-N-propyl silane adsorbent. The supernatant was evaporated under nitrogen until dry and finally reconstituted in methanol. Chromatographic separation was performed on an Agilent Poroshell 120 EC-C18 column in methanol (mobile phase A)-water (optimized for 0.1% acetic acid and 10 mM ammonium acetate, mobile phase B) at a 0.3 mL·min−1 flow rate. Electrospray ionization and positive ion multiple reaction monitoring were used for detection. The time for of analysis was 13 min. The calibration curves range of tacrolimus, rapamycin, everolimus and pimecrolimus were in the range of 1–100 ng·mL−1, mycophenolate mofetil in the range of 0.1–10 ng·mL−1 and mycophenolic acid at 10–1000 ng·mL−1. All correlation coefficients were >0.993. The coefficients of variation (CV, %) for inter-day and intra-day precision were less than 10%, while the spiked recoveries were in the range of 92.1% to 116%. Our method was rapid, sensitive, specific, and reproducible for the simultaneous determination of six immunosuppressants in human whole blood. Importantly, our approach can be used to monitor drug concentrations in the blood to facilitate disease treatment.
Steroidal Antimetabolites Protect Mice against Trypanosoma brucei
Authors: Chaudhuri Minu , Singha Ujjal K. , Vanderloop Boden H. , Tripathi Anuj , Nes W. David
Published: June 2022

Trypanosoma brucei, the causative agent for human African trypanosomiasis, is an emerging ergosterol-dependent parasite that produces chokepoint enzymes, sterol methyltransferases (SMT), not synthesized in their animal hosts that can regulate cell viability. Here, we report the lethal effects of two recently described natural product antimetabolites that disrupt Acanthamoeba sterol methylation and growth, cholesta-5,7,22,24-tetraenol (CHT) and ergosta-5,7,22,24(28)-tetraenol (ERGT) that can equally target T. brucei. We found that CHT/ERGT inhibited cell growth in vitro, yielding EC50 values in the low nanomolar range with washout experiments showing cidal activity against the bloodstream form, consistent with their predicted mode of suicide inhibition on SMT activity and ergosterol production. Antimetabolite treatment generated altered T. brucei cell morphology and death rapidly within hours. Notably, in vivo ERGT/CHT protected mice infected with T. brucei, doubling their survival time following daily treatment for 8–10 days at 50 mg/kg or 100 mg/kg. The current study demonstrates a new class of lead antibiotics, in the form of common fungal sterols, for antitrypanosomal drug development.
Carnosic Acid Encapsulated in Albumin Nanoparticles Induces Apoptosis in Breast and Colorectal Cancer Cells
Authors: Khella Katren F. , Abd El Maksoud Ahmed I. , Hassan Amr , Abdel-Ghany Shaimaa E. , Elsanhoty Rafaat M. , Aladhadh Mohammed Abdullah , Abdel-Hakeem Mohamed A.
Published: June 2022

Carnosic acid (CA) is a natural phenolic compound with several biomedical actions. This work was performed to study the use of CA-loaded polymeric nanoparticles to improve the antitumor activity of breast cancer cells (MCF-7) and colon cancer cells (Caco-2). CA was encapsulated in bovine serum albumin (BSA), chitosan (CH), and cellulose (CL) nanoparticles. The CA-loaded BSA nanoparticles (CA-BSA-NPs) revealed the most promising formula as it showed good loading capacity and the best release rate profile as the drug reached 80% after 10 h. The physicochemical characterization of the CA-BSA-NPs and empty carrier (BSA-NPs) was performed by the particle size distribution analysis, transmission electron microscopy (TEM), and zeta potential. The antitumor activity of the CA-BSA-NPs was evaluated by measuring cell viability, apoptosis rate, and gene expression of GCLC, COX-2, and BCL-2 in MCF-7 and Caco-2. The cytotoxicity assay (MTT) showed elevated antitumor activity of CA-BSA-NPs against MCF-7 and Caco-2 compared to free CA and BSA-NPs. Moreover, apoptosis test data showed an arrest of the Caco-2 cells at G2/M (10.84%) and the MCF-7 cells at G2/M (4.73%) in the CA-BSA-NPs treatment. RT-PCR-based gene expression analysis showed an upregulation of the GCLC gene and downregulation of the BCL-2 and COX-2 genes in cells treated with CA-BSA-NPs compared to untreated cells. In conclusion, CA-BSA-NPs has been introduced as a promising formula for treating breast and colorectal cancer.
Peptidyl-Resin Substrates as a Tool in the Analysis of Caspase Activity
Authors: Bąchor Remigiusz
Published: June 2022

Caspases, proteolytic enzymes belonging to the group of cysteine proteases, play a crucial role in apoptosis. Understanding their activity and substrate specificity is extremely important. Fluorescence-based approaches, including fluorogenic substrates, are generally used to confirm cleavage preferences. Here we present a new method of substrate specificity and activity analysis based on the application of fix-charge tagged peptides located on the resin. The proteolysis of peptide bond on the resin, occurring even with low efficiency, results in the formation of N-terminal fragments of model peptide containing ionization enhancers in the form of quaternary ammonium groups, allowing for ultrasensitive and reliable analysis by LC-MS/MS. The possibility of application of the proposed solution was tested through the analysis of substrate specificity and activity of caspase 3 or 7. The obtained results confirm the known substrate specificity of executioner caspases. Our solution also allowed us to observe that caspases can hydrolyze peptides shorter than those presented to date in the scientific literature.
Prognostic Ability of Enhancer RNAs in Metastasis of Non-Small Cell Lung Cancer
Authors: Liu Jun , Jia Jingyi , Wang Siqiao , Zhang Junfang , Xian Shuyuan , Zheng Zixuan , Deng Lin , Feng Yonghong , Zhang Yuan , Zhang Jie
Published: June 2022

(1) Background: Non-small cell lung cancer (NSCLC) is the most common lung cancer. Enhancer RNA (eRNA) has potential utility in the diagnosis, prognosis and treatment of cancer, but the role of eRNAs in NSCLC metastasis is not clear; (2) Methods: Differentially expressed transcription factors (DETFs), enhancer RNAs (DEEs), and target genes (DETGs) between primary NSCLC and metastatic NSCLC were identified. Prognostic DEEs (PDEEs) were screened by Cox regression analyses and a predicting model for metastatic NSCLC was constructed. We identified DEE interactions with DETFs, DETGs, reverse phase protein arrays (RPPA) protein chips, immunocytes, and pathways to construct a regulation network using Pearson correlation. Finally, the mechanisms and clinical significance were explained using multi-dimensional validation unambiguously; (3) Results: A total of 255 DEEs were identified, and 24 PDEEs were selected into the multivariate Cox regression model (AUC = 0.699). Additionally, the NSCLC metastasis-specific regulation network was constructed, and six key PDEEs were defined (ANXA8L1, CASTOR2, CYP4B1, GTF2H2C, PSMF1 and TNS4); (4) Conclusions: This study focused on the exploration of the prognostic value of eRNAs in the metastasis of NSCLC. Finally, six eRNAs were identified as potential markers for the prediction of metastasis of NSCLC.
Sandwich Enzyme-Linked Immunosorbent Assay for Quantification of Callose
Authors: Mustafa Abubakar S. , Ssenku Jamilu E. , Ssemanda Paul , Ntambi Saidi , Dinesh-Kumar Savithramma P. , Tugume Arthur K.
Published: June 2022

The existing methods of callose quantification include epifluorescence microscopy and fluorescence spectrophotometry of aniline blue-stained callose particles, immuno-fluorescence microscopy and indirect assessment of both callose synthase and β-(1,3)-glucanase enzyme activities. Some of these methods are laborious, time consuming, not callose-specific, biased and require high technical skills. Here, we describe a method of callose quantification based on Sandwich Enzyme-Linked Immunosorbent Assay (S-ELISA). Tissue culture-derived banana plantlets were inoculated with Xanthomonas campestris pv. musacearum (Xcm) bacteria as a biotic stress factor inducing callose production. Banana leaf, pseudostem and corm tissue samples were collected at 14 days post-inoculation (dpi) for callose quantification. Callose levels were significantly different in banana tissues of Xcm-inoculated and control groups except in the pseudostems of both banana genotypes. The method described here could be applied for the quantification of callose in different plant species with satisfactory level of specificity to callose, and reproducibility. Additionally, the use of 96-well plate makes this method suitable for high throughput callose quantification studies with minimal sampling and analysis biases. We provide step-by-step detailed descriptions of the method.