bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Kandungan Cemaran Bakteri Coliform Pada Air Rendaman Tahu di Pabrik Tahu Wilayah Kalideres Jakarta Barat
Authors: AF Adelia Febriyossa , MIK Melania Immaculata Koten
Published: December 2022

Tofu is a food source containing high vegetable protein. Tofu is in great demand by most of the Indonesian people because it contains high nutrition and the price is relatively cheap and affordable. To produce tofu requires good hygiene and sanitation, such as providing clean raw materials and water sources that are free from contamination. To determine whether the water source has been contaminated with microbes or not, the Coliform bacteria indicator in the water sample is used. In testing Coliform bacterial contamination in water or raw materials, the Most Probable Number (MPN) method is used to calculate the amount or level of microbial contamination in determining the quality or quality of water and raw materials by referring to the MPN table. This study aims to determine the level of Coliform bacteria contamination in tofu soaking water in a tofu factory in the Kalideres area of ??West Jakarta. The design of this research is a descriptive laboratory type which was carried out in March-June 2020 at the Indonesian Social Solidarity STIKes Laboratory. Based on the results of the research on the presumptive test, it was found that 1 positive tube was contaminated with Coliform in the KR tofu factory and 3 positive tubes at the DN factory. The presumptive test was continued to confirmatory test and found 5 positive tubes polluted at the DD factory, 2 positive tubes at the KR factory and 3 positive tubes at the DN factory. The positive tube from the confirmation test was calculated for the contamination index value (MPN) and obtained 21000 mpn/ml for the DD factory, 4000 mpn/ml for the KR factory and 9000 mpn/ml for the DN factory. It can be concluded that there is Coliform bacterial contamination in all tofu soaking water samples in 3 tofu factories with the MPN index value having passed the contamination threshold.
Modeling the Parenting Stress of Mothers of Autistic Children Based on Mindfulness and Meta-parenting With the Mediation of Self-efficacy
Authors:   Department of Psychology, Roudehen Branch, Islamic Azad University, Roudehen, Iran., Amirlou Farahnaz , Saberi Hayedeh ,   Department of Psychology, Roudehen Branch, Islamic Azad University, Roudehen, Iran., Hakim Shoushtari Mitra ,   Department of Child and Adolescent Psychiatry, Faculty of Behavioral Sciences and Mental Health (Tehran Institute of Psychiatry), Iran University of Medical Sciences, Tehran, Iran.
Published: December 2022

Objectives: It is highly important to identify the factors related to the general quality of life of parents, especially mothers of children with autism. Therefore, the present study aims to predict parenting stress based on mindfulness and meta-parenting considering the mediating role of parental self-efficacy of mothers of children with autism. Methods: The present correlational study was conducted using the structural equation modeling (SEM) method. The study population included all mothers of 2- to 14-year-old children with autism referring to the therapeutic clinics in Tehran City, Iran, in 2021. Of this population, a total of 250 individuals were selected using the purposive sampling method and then responded to the parenting stress (PSI-SF), mindfulness (MAAS), meta-parenting (M-PQ), and parental self-efficacy (PSAM) questionnaires. The data were analyzed via the SEM method in the AMOS 21.0 software. Results: The total path coefficient between mindfulness (P‌<‌0.01, β‌=‌-‌0.671), meta-parenting (P‌<‌0.01, β‌=‌-0.256), and parental self-efficacy (P‌<‌0.01, β‌=‌-‌0.352), and parenting stress was negative and significant. Also, the indirect path coefficient between parenting stress and mindfulness (P‌<‌0.01, β‌=‌-‌0.153) and meta-parenting (P‌<‌0.01, β‌=‌ -‌0.116) was negative and significant at the level of 0.01. Moreover, the squared multiple correlation coefficient (R2) for the parenting stress variable was obtained at 0.63. Conclusion: Mindfulness and meta-parenting can predict parenting stress in mothers of children with autism both directly and with the intermediation of parental self-efficacy. Therefore, to develop interventions for preventing high levels of parenting stress, it is necessary to utilize the findings to improve parents’ parent-child relationships.
The Effect of Eight Weeks of TRX Resistance Training Along With Using Taurine Supplementation on Glycemic Indices of Type 2 Diabetic Females
Authors:   Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran., Samadpour Masouleh Shohreh , Siahkouhian Marefat ,   Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran., Jafarnezhadgero Amirali ,   Department of Sport Management and Biomechanics, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran., Farzizadeh Reza ,   Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.
Published: December 2022

Background and Aims Type 2 diabetes is the most common endocrine disease in the world. Various methods, such as medication and exercise can be helpful, although information on the issue is scarce. Therefore, the aim of the present study was to investigate the effect of eight weeks of TRX resistance training along with using taurine supplementation on glycemic indices of type 2 diabetic females. Methods The sample of the present study consisted of 40 middle-aged females with inactive diabetes with an age range of 40 to 60 years. Subjects were randomly divided into four groups of ten, including the training group, supplement group, training+supplement group, and control group. Fasting blood glucose (FBS) and glycosylated hemoglobin (HBA1C) levels were measured before and after the training period. The duration of the training program for the training group was eight weeks for three sessions per week Results The results showed that HBA1C levels decreased in the post-test compared to the pre-test in the supplement (P=0.027), exercise (P=0.001), and supplement+exercise (P=0.001) groups. While the levels of this variable did not show any significant difference in the control group in the post-test compared to the pre-test (P>0.05). The results showed that FBS levels in the exercise group were statistically lower than in the supplement + exercise group (P=0.012). Conclusion According to the findings, it can be concluded that resistance training using the TRX method along with taurine supplement can reduce resting FBS and glycosylated hemoglobin (HBA1C) levels in type 2 diabetic women and increase women’s health.
Development of 3D Model and Display on Web Using Open-Source Technology
Authors: Sushil Chandra  , Udai Raj  , Rajeev Sonkar  , Pragati Srivastava  , Ayushi
Published: December 2022

It is basically done on the concept for bringing the 2D area view to 3D area view . The problem arises in the 2D view area is that there is only perspective angle of sight which may occurs problem after some time if we use it as a blueprint. 3D-model overcome this problem. As 3D model has various perspective angle of sight . If in the blueprint there is any such model which is not suitable at that location, we can easily remove it from there and place it on the new location where it is needed.
For this we use Blender for 3D modelling and later on displayed on the web using Cesium and Three.js.
Phytochemical Screening and Comparative Study of Antioxidant Activity of Different Parts of Mulberry Plant
Authors: Vidya. K. Kakade  , Dr. Abhijeet S. Kulkarni  , Kalpana Disale  , Mayuri Patil  , Pratik Patil  , Snehal Gaikwad  , Trupti Khadekar
Published: December 2022

Mulberry is commonly used for silkworm rearing due to the presence of chemo-factors such as morin and sitosterol in the leaves. Mulberries are important in containing free radicals and pro- oxidants produced in the body as a result of metabolism and phagocytosis. Pharmacological uses of mulberry include phytochemical composition, antioxidant and medicinal properties. The pharmacological activity of mulberry plants can be predicted by the identification of phytochemicals. Although various modern techniques are used to determine phytochemicals, qualitative tests are still used for preliminary phytochemical screening of plants. Alkaloids, carbohydrates, proteins, flavonoids, phenolic compounds, saponins, tannins, quinones, anthraquinones, and other phytochemicals. The purpose of this research was to determine the antioxidant activity of the mulberry plant's fruits, stems, and leaves. The presence of antioxidant properties in the extract of mulberry plant was confirmed using the DPPH method.
Scientific Biography of Volodymyr Hoshovskyi: to the Centenary Jubilee
Authors:   Vasyl Stefanyk National Scientific Library of Ukraine in Lviv, Pasichnyk Volodymyr
Published: December 2022

The article deals with the scientific biography of V. Hoshovskyi, mostly con- cerning the 50-year period of his creative life, spanning the years of 1946–1996. The circumstances of his life are discussed, which influenced the upbringing and profes- sional background, among other. his manners and lifestyle, studies in gymnasium and University, the vast scientific educational background and erudition. The utmost importance is given to ethnography studies, musical dialectology and Slavic studies. The biography outline of V. Hoshovskyi spans several distinctive periods. First – Trans-Carpathian (1946–1960), concerned which ethnography, musical aesthetics, regional studies and elaboration of the principles of ethnomusicology, including the music dialectological studies. Second – Lviv period (1961–1974), which pinnacled his elaborate studies in music dialectology and comparative Slavic studies. The important part of this period is the formation in his scientific studies the basics of cybernetic ethnomusicology. The third period spans 1975–1996, which can be subdivided into two sub peri- ods: the Armenian one (1975–1986), which is specifically concerned with elaboration of cybernetic ethnomusicology, and the Lviv one, which spans the last decade in life of the musicologist – 1986–1996 and is concerned with further elaborations in cy- bernetic ethnomusicology, as well as public relations and musicology courses by V. Hoshovskyi. We can stress that V. Hoshovskyi were not only the promoter of up to-the-mo- ment tendencies of European musicology, but also the elaborator and promoter of some innovative studies. The scientific studies of V. Hoshovsky has became the notable contribution in all-Ukrainian and European musical culture. Keywords: Volodymyr Hoshovskyi, ethnography, ethnomusicology, music dia- lectology, Slavic studies, cybernetic ethnomusicology.
Cardiovascular Profile And Risk of Patients During Non-Cardiovascular Surgery Scheduled at Sylvanus Olympio University Hospital in Lome
Authors:   Teaching and Research Unit in Cardiology and Vascular Diseases, University of Kara, Togo, PIO Machihude , BAKAI Abalo Mario , ADZODO Ablawa Akpé , ATTA, Borgotia D , AFASSINOU Yaovi Mignazonzon , TCHEROU Tchaa , ASSENOUWE Sarakawabalo , SAMA Hamza D , PESSINABA Soulemane , DJALOGUE, Lihanimpo , Wiyaou Dieu-Donné KAZIGA , Yoba Gnimdou AKALA
Published: December 2022

Objective: To describe the cardiovascular profile and the preoperative cardiovascular risk stratification prior to scheduled non-cardiac surgeries at CHU SO. Materials and methods: This is a retrospective and descriptive study on the records of patients received in consultation in the Cardiology and anesthesia departments of the CHU-SO of Lomé from March 2014 to October 2019. Results: A total of 220 patient records were selected. The mean age was 57.1±14.4. In 81% of cases, these are minor predictors; intermediate predictors are found in 9.1% of cases and in 1.4% they are major factors. Patients were classified as ASA1 (50.0%), ASA 2 (39.1%), ASA 3 (9.6%), and ASA4 (41.3%). As age increased, patients had a major predictor factor or were classified as ASA3 and ASA4. The predictors of minor operative risk were hypertension (70.3%) followed by age correlated with sex (48.2%) in men, metabolic syndrome (21.8%), and left ventricular hypertrophy (LVH) (21.0 %). Intermediate predictors were dominated by diabetes (9.1%), chronic myocardial ischemia (6.8%), and RI (4.5%). Patients with diabetes (9.1%) or morbid obesity (7.7%) were classified as ASA3 Heart failure (1.4%) and renal failure (4.5%) classified patients as ASA4. Conclusion: The predictors of cardiovascular risk for NCS are multiple and are observed in the same patients. The overall operative risk is moderate to medium.
Aneurysm of The Superior Mesenteric Artery Associated With A Cardio-Renal Syndrome About A Case in Mali
Authors:   1 Cardiac Surgery Service Andre Festoc, Luxembourg Mother-Child Teaching Hospital, Bamako, Mal, Doumbia Modibo , Djiguiba Karamoko , Toure Mamadou , Dolo Abdou , Diarra Baba Ibrahima , Coulibaly Bréhima , Daffe Sanoussy , Coulibaly Mahamadoun , Keita, Asmao , Diallo Binta , Traore Salia , Koita Siriman A , Togola Birama , Togo Seydou , Diarra Mamadou B , Yena Sadio
Published: December 2022

The authors report a rare association of superior mesenteric artery aneurysm and cardio-renal syndrome is a complex clinical and therapeutic entity of cardiovascular and renal pathology whose prognosis depends on the precocity of management. We report a case of superior mesenteric artery aneurysm in a 36-year-old hypertensive patient, suffering from terminal renal failure complicating with stage 2 cardiorenal syndrome who underwent a hemodialysis session and a literature review.
MESLEK YÜKSEKOKULU ÖĞRENCİLERİNİN TEMEL İSG EĞİTİMLERİNİN İSG ALGISI OLUŞTURULMASINA ETKİSİ: BİR ÜNİVERSİTE ÖRNEĞİ
Authors: ÇIRAKOĞLU KELLECİ Sevil , AKALP Hüsre Gizem , SAKLANGIÇ Uğur , TAŞCI Hüdayi
Published: December 2022

Üretimin dijital dönüşüme geçmesinin ardından değişen çalışma hayatında, yoğun iş temposu nedeniyle giderek artan bir öneme sahip olan İş Sağlığı ve Güvenliği konusunun temel amacı kişileri iş kazalarından ve meslek hastalıklarından koruyarak, sağlıklı ve güvenli çalışma ortamlarının oluşturulmasıdır.
Bu çalışmanın temel amacı, bir üniversitenin Teknik Bilimler Meslek Yüksekokulunda öğrenim gören öğrencilerin aldıkları İş Sağlığı ve Güvenliği Dersleri sonucunda algı düzeylerinin belirlenmesidir. Betimsel analiz niteliğindeki bu araştırma, üniversitenin Teknik Bilimler Meslek Yüksek Okulu’nda öğrenim gören beş ayrı programdan toplam 350 katılımcı ile gerçekleştirilmiştir. 5’li Likert tipinde 33 maddelik anket formu katılımcılara ders esnasında Google Forms linki gönderilmiştir.K ullanılabilir 333 form değerlendirmeye alınmıştır. Katılımcıların %47’si meslek lisesi mezunudur. %46,8’inin 6331 sayılı İş Sağlığı ve Güvenliği Kanununu hakkında bilgisi vardır. %96’sı İş Sağlığı ve Güvenliğine dikkat edilmesinin iş verimine olumlu katkıda bulunacağını belirtmiştir. İş sağlığı ve güvenliği programında öğrenim gören öğrencilerin programları gereği İSG algıları beklenilen şekilde yüksek çıkmıştır. Araştırmanın sonucuna göre, aldıkları İş Sağlığı ve Güvenliği dersi, öğrencilerin kendi ilgi alanlarında iş sağlığı ve güvenliği ile ilgili olarak daha bilgili oldukları sonucunu ortaya çıkarmaktadır.
Çocuk Odaklı Haberlerin Ana Akım Ve Alternatif Medyada Sunumu: İzmir Depremi Örneği
Authors: KARATAŞ Emrah Onur
Published: December 2022

İzmir’in Seferihisar ilçesinde 30 Ekim 2020’de yaşanan 6,6 şiddetindeki deprem, gerek uzmanlar tarafından yapılan açıklamalar özelinde gerekse enkaz altında kalan bireyleri konu alması nedeniyle, basında oldukça yer almış, çocuklara dair haberler, depremin yıkıcılığının önüne geçerek gündem haline gelmiştir. Çocukların medyada nasıl, ne kadar ve ne şekilde yer aldığı, söz konusu yayın organının çocuk hakları odaklı olup olmadığı, çocuklara yönelik tutumların oluşmasında ve çocukların gelecekteki hayatlarını şekillendirmeleri noktasında oldukça önemlidir. Bu doğrultuda, çocuk odaklı habercilik ilkeleri kapsamında, çocukların medya metinlerinde kullanımına yönelik kısıtlamalar söz konusudur. Çalışmanın temel problemini, medya organlarının doğal afet ve felaket haberlerinin üretim süreçlerinde çocukları haberin öznesi olarak nasıl konumlandırdığı kaygısı oluşturmaktadır. Çalışmanın amacı, ana akım medya ve alternatif medyada karşılaştırmalı olarak çocuk haberlerinin nasıl sunulduğunu ortaya koymak ve söylem yapılarını tespit etmektir. Ana akım medya organlarından Sabah ve Hürriyet seçilirken, alternatif medya organlarından ise Bianet ve Gazete Duvar örneklem olarak seçilmiştir. Haberler depremin gerçekleştiği tarihi takip eden ilk hafta ile sınırlandırılarak, 1-7 Kasım 2020 tarihleri arasında yayınlanan haberler söylem analizi yöntemi ile incelenmiştir. Çalışmanın örneklemini oluşturan söz konusu yayın organlarının, çocuk odaklı haberleri konu edinirken ana akım medya kanallarının daha dramatik söylemlerden faydalandığı görülmektedir.
Cautious considerations for using multiple covariate distance sampling and seafloor terrain for improved estimates of rockfish density
Authors: Echave KB , Pirtle JL , Heifetz J , Shotwell K
Published: December 2022

Distance sampling is one of the most widely used methods for adjusting fish counts for detectability, which allows the estimation of absolute density. Further, abundance can be estimated spatially beyond the transect width with density surface models. The present study used a density surface modelling approach with survey data from line transects and terrain metrics from multibeam acoustic bathymetry data, integrated with a Geographic Information System, to estimate the population density of Pacific Ocean perchSebastes alutus(POP) within 6 sampling areas in the Gulf of Alaska (GOA) at 2 sampling scales: local and landscape. For adult POP, the final density model included depth as a significant predictor for density estimation at both the local and landscape scale. Additional factors included sponge coverage (local scale), aspect eastness and seafloor slope (landscape scale). Predicted densities of adult POP are highest on low (<5°) eastern-facing slopes with high sponge coverage (>75%), at depths of 100 to 200 m. By using habitat-based density models with data collected from line transect distance sampling and multibeam acoustic seafloor mapping surveys, we provide an alternative method for estimating rockfish abundance in untrawlable areas, which may help improve rockfish stock assessments and essential fish habitat descriptions and maps.
Gas Containing Lumbar Synovial Cyst: A Case Report
Authors:   Department of Neurosurgery, BHT Clinic Istanbul TEMA HASTANESİ Atakent Mahallesi 4. Cadde No:36 PK:34307 Küçükçekmece/İSTANBUL TURKEY. Email, AC Iplikçioğlu ,   acelal@doctor.com, H Karabağ ,   Department of Neurosurgery, Harran Üniversity, Şanlıurfa, Turkey. Email: hamzakarabag@ yahoo.com.
Published: December 2022

Spinal synovial cyst is a benign cystic mass originated from the facet joints. It is usually associated with degenerative spondylosis or spondylolisthesis and occurs frequently in lumbar region [1,2].
Acquired Language Disorders in Bilinguals
Authors: Cargnelutti Elisa , Tomasino Barbara , Fabbro Franco
Published: December 2022

The literature reports an increased number of aphasias involving bilingual people. Dealing with bilingual aphasia requires particular attention from the diagnostic to the therapeutic phase. In this review, the authors describe the possible impairment patterns, which could be different between the two languages and be characterised by specific deficits and sometimes unexpected profiles. The role of some crucial factors in determining the observed deficits and impairment patterns is illustrated, for instance age of appropriation and proficiency. An early versus late language appropriation recruits different brain processes and hence different brain structures. In general, a greater vulnerability is observed for the late-learned languages, although a high proficiency or use and exposure appear to prevent language impairment even in the case of late appropriation. The authors also discussed the role of other intervening factors, such as emotional–motivational aspects, which could explain unusual profiles. Furthermore, language deficits specific to bilingualism, such as pathological mixing and switching and translation problems were described. In this respect, the authors underlined the fundamental involvement of cognitive control mechanisms and of the role of the brain structures associated with this. Lastly, the clinical practice issues in bilingual aphasia were outlined, underlining the need for a careful diagnosis. This should take into account the patient’s language history in order to avoid biased assessments and instead promote the setup of effective intervention programmes.
Chronic viral infection and toxic epidermal necrolisis (Lyell´s syndrome)
Authors: Krivosheev A. B. , Ermachenko T. V. , Khavin P. P. , Zakharov K. V. , Okrugina A. A. , Krivosheeva I. A. , Ammosova I. V.
Published: December 2022

Two cases of toxic epidermal necrolysis (Lyell's syndrome) are described. The introduction discusses the relevance of this problem. Emphasis is placed on the differential diagnosis of Lyell's syndrome with Stevens-Johnson syndrome, since both syndromes are difficult to distinguish in the debut. As differential diagnostic criteria, it is recommended to take into account the presence of Nikolsky's symptom and the area of involvement in the pathological process of the skin more than 30%. In the manifestation of Lyell's syndrome, the role of genetic predisposition is not excluded. The presented observations indicate that the first clinical symptoms are identical. In both cases, the disease was triggered by medications first used. The common background was viral infection: long-term chronic HCV-infection in combination with herpes zoster and HIV-infection without antiretroviral therapy. Our observations expand our understanding of the likely predisposing factors of Lyell's syndrome, which can include acute and long-term chronic viral infections.
The Use of Trauma Healing in Treating PTSD in Post-Disaster Victims: A Narrative Review
Authors: Sutini Titin , Emaliyawati Etika , Sodiqiah Siti Nurfazri , Mawaddah Salwa , Purba Fitri Yoselin Yamadea , Rizkiani Agni , Amatullah Dhiyaa Ulhaq
Published: December 2022

Aims: Natural disasters can have an impact on the material (physical), psychological and social aspects. The traumatic experience is a psychological impact on victims that are often found. traumatic experience or traumatic stress if it lasts for 6 months will result in PTSD (Post Traumatic Stress Disorder). There are various interventions to treat PTSD, this intervention is known as trauma healing. Trauma healing can be one of the appropriate rehabilitation tools for disaster victims, especially to heal post-disaster trauma. The purpose of the study is to find out what trauma healing interventions are in handling PTSD in post-disaster victims.
Method: The method used is a narrative review with PRISMA 2009 guidelines. The search is based on Garuda, EBSCO, Proquest, and PubMed. The studies reviewed were RCT research, quasi-experiment, and qualitative research in English and Indonesian with full text, as well as intervention studies within the last 7 years of publication from 2015  to 2021.
Result: Trauma healing that can be given and proven effective to treat or reduce PTSD symptoms is CBT (Cognitive Behavioral Therapy), remembrance therapy, dhikr and play therapy. The results of the search used turned out to be for CBT therapy and play therapy were more effective in reducing PTSD in children, while dhikr was effective in adults, and remembrance therapy was very suitable for adults who experienced PTSD with other physical problems such as hypertension.
Conclusions: Therapy as post-disaster trauma healing to overcome PTSD is divided into two, namely therapy for children and adults. Therapy for children is playing therapy and CBT, while for adults there is dhikr, and remembrance therapy is used for adults who experience physical disorders such as hypertension.
RANCANG BANGUN SMART TOURISM PENGELOLAAN PARIWISATA KABUPATEN MALANG BERBASIS WEB GIS
Authors: Silmi Muhammad Hifzhan , Yuri Ariyanto  , Ade Ismail
Published: December 2022

Seiring berjalannya waktu, teknologi informasi berkembang semakin pesat. Salah satunya yang sedang gencar dikembangkan di berbagai daerah, kota, maupun negara adalah konsep Smart City. Salah satu penerapannya adalah mewujudkan smart tourism, dimana bertujuan untuk mempermudah sampainya informasi tentang peta lokasi, situasi, dan segala informasi dari objek-objek wisata di suatu daerah. Maka dari itu, penulis melakukan penelitian dengan mengembangkan suatu Sistem Informasi Geografis berbasis website yang diintegrasikan dengan Sistem Pendukung Keputusan menggunakan metode Analytical Hierarchy Process untuk mempermudah penggunanya dalam mengelola data dan mendapatkan informasi detail dan rekomendasi destinasi objek wisata terbaik di Kecamatan Dau. Hasil pengujian pada penelitian ini menunjukkan bahwa menggunakan metode AHP sudah cukup membantu calon wisatawan dalam menentukan objek wisata terbaik. Sistem Informasi Geografis yang dibangun juga dibuat simple namun lengkap. Berdasarkan pengujian yang dilakukan, disimpulkan bahwa semakin banyak jumlah data kriteria dan alternatif yang digunakan dalam implementasi metode AHP, maka juga dapat berpengaruh dalam peningkatan akurasi.
Self-assembled microtissues loaded with osteogenic MSCs for in vivo bone regeneration
Authors: Li Hui , He Zihao , Li Wenjing , Li Jiao Jiao , Lin Jianhao , Xing Dan
Published: December 2022

Bone regeneration strategies based on mesenchymal stem cell (MSC) therapy have received widespread attention. Although MSC incorporation into bone scaffolds can help with the repair process, a large number of studies demonstrate variable effects of MSCs with some noting that the inclusion of MSCs does not provide better outcomes compared to unseeded scaffolds. This may in part be related to low cell survival following implantation and/or limited ability to continue with osteogenic differentiation for pre-differentiated cells. In this study, we incorporated MSCs into gelatin microcryogels to form microtissues, and subjected these microtissues to osteogenic induction. We then mixed as-formed microtissues with those subjected to 6 days of osteogenic induction in different ratios, and investigated their ability to induce in vitro and in vivo osteogenesis during self-assembly. Using a full-thickness rat calvarial defect model, we found that undifferentiated and osteogenically induced microtissues mixed in a ratio of 2:1 produced the best outcomes of bone regeneration. This provides a new, customizable cell-based therapeutic strategy for in vivo repair of bone defects.
2D–2D heterostructure g-C3N4-based materials for photocatalytic H2 evolution: Progress and perspectives
Authors: Mehmood Rashid , Ahmad Zia , Hussain Muhammad Bilal , Athar Muhammad , Akbar Ghulam , Ajmal Zeeshan , Iqbal Sikandar , Razaq Rameez , Ali Mohammad Arif , Qayum Abdul , Chishti Aadil Nabi , Zaman Fakhr uz , Shah Rahim , Zaman Shahid , Adnan
Published: December 2022

Photocatalytic hydrogen generation from direct water splitting is recognized as a progressive and renewable energy producer. The secret to understanding this phenomenon is discovering an efficient photocatalyst that preferably uses sunlight energy. Two-dimensional (2D) graphitic carbon nitride (g-C3N4)-based materials are promising for photocatalytic water splitting due to special characteristics such as appropriate band gap, visible light active, ultra-high specific surface area, and abundantly exposed active sites. However, the inadequate photocatalytic activity of pure 2D layered g-C3N4-based materials is a massive challenge due to the quick recombination between photogenerated holes and electrons. Creating 2D heterogeneous photocatalysts is a cost-effective strategy for clean and renewable hydrogen production on a larger scale. The 2D g-C3N4-based heterostructure with the combined merits of each 2D component, which facilitate the rapid charge separation through the heterojunction effect on photocatalyst, has been evidenced to be very effective in enhancing the photocatalytic performance. To further improve the photocatalytic efficiency, the development of novel 2D g-C3N4-based heterostructure photocatalysts is critical. This mini-review covers the fundamental concepts, recent advancements, and applications in photocatalytic hydrogen production. Furthermore, the challenges and perspectives on 2D g-C3N4-based heterostructure photocatalysts demonstrate the future direction toward sustainability.
Inter ocean basin moisture fluxes and the onset of the summer rainy season over southern Mexico
Authors: Magaña Víctor , Diaz Sonia
Published: December 2022

The onset of the rainy season in monsoonal climates is a key element for the development of water related activities. In southern Mexico, it starts in late May or early June, while in western and northwestern part of the country in begins between early and mid-July. Previous studies indicate that it depends on the meridional position of the eastern Pacific Inter Tropical Convergence Zone (ITCZ). By mean of analyses of the Vertically Integrated Moisture Flux, the low level atmospheric dynamics in the tropical Americas is analyzed to establish the temporal evolution of inter ocean moisture fluxes that lead to the onset of the summer rainy season in southern Mexico. The onset of the rainy season depends on the longitudinal position of the center of maximum convective activity in the ITCZ, which induces a quasi-stationary low-level cyclonic circulation that results in moisture fluxes to the Mexican Pacific coast and rains. The Caribbean Low-Level Jet (CLLJ) is a mechanism that modulates inter ocean basin moisture fluxes into the eastern Pacific ITCZ and the location of the maximum convective activity in this system. We propose that the sequence of events that leads to the onset of the rainy season in southern Mexico is: (i) an episode of strong moisture flux from the Caribbean Sea into the eastern Pacific ITCZ, (ii) a region of intense convective activity in the ITCZ that triggers a quasi-stationary low-level cyclonic circulation which in turn, induces moisture flux into southern Mexico, and (iii) the development of convective activity in southern Mexico that determines the onset of the rainy season. The intensification of the CLLJ from June to July also results in a westward displacement of the region of maximum convection within the ITCZ that leads to changes in the atmospheric circulations and moisture fluxes that determine the onset of the rainy season at higher latitudes in the Mexican Pacific coast.
Classroom influence—Do students with high autistic traits benefit from their classmates’ social skills?
Authors: Nenniger Gina
Published: December 2022

Introduction

Children and adolescents with intellectual disability (ID) and high levels of autistic traits often attend special needs classrooms where they spend a lot of time with other students who demonstrate diverse impairments and competencies. Research in typical development shows that classmates and the classroom composition in terms of specific classmate competencies can have a strong impact on individual social development. In this context, classmates’ social skills are of particular interest, as they are associated with successful social interaction and the ability to establish and maintain social relationships. Based on these associations, the present study investigated whether the levels of autistic traits and social skills in children and adolescents with ID and high levels of autistic traits are influenced by their classmates’ levels of social skills.

Methods

A longitudinal design was used, with the first measurement point at the beginning of the school year and the second at the end of the school year. School staff members provided information on 330 students with ID and high levels of autistic traits (20.6% girls; mean age 10.17 years, SD = 3.74) who were schooled in 142 classrooms across 16 Swiss special needs schools.

Results

Results showed that students’ individual levels of autistic traits and social skills at T2 were not predicted by the classroom level of social skills at T1 when controlling for individual levels of autistic traits, individual levels of social skills, gender, age, and general levels of functioning at T1.

Discussion

Considering the present findings, perspectives for further research and support of children and adolescents with ID and high levels of autistic traits within the classroom context are discussed.
A pressure-state-response-support framework for assessing regional water environmental carrying status
Authors: Hu Aqin , Li Qingsheng
Published: December 2022

The water environment carrying status (WECS) is the embodiment of the ability of an aquatic ecosystem to maintain healthy and stable development; as such, it is an essential factor in the sustainable social and economic development of a region. To evaluate the water environment carrying status of a region, a new Pressure-State-Response-Support (PSRS) framework is proposed firstly. Secondly, a water carrying status index system containing 21 indicators is constructed based on this new model, and a water environment carrying status index (WECSI) is introduced to describe the water environment carrying status. Then, projection pursuit and entropy methods are used to calculate the water environment carrying status index of eight cities along the Yangtze River in Jiangsu province as examples. The results reveal that although the government has made substantial effort, the pressure subsystem is still dominated the changes of water environment carrying status, the eight cities still face significant pressure. The analysis indicated that government departments should commit to alleviating pressure and monitoring and supervising surface water quality while continuing to maintain a supporting force. The proposed framework and methodology is sound and effective for measuring the carrying status of regional water environment, and the methods can provide guidance for the Ecological Environment Department in ensuring the carrying capabilities of water environment and achieving sustainable development mission at regional level.
Deficiency of ribosomal protein S26, which is mutated in a subset of patients with Diamond Blackfan anemia, impairs erythroid differentiation
Authors: Piantanida Noemy , La Vecchia Marta , Sculco Marika , Talmon Maria , Palattella Gioele , Kurita Ryo , Nakamura Yukio , Ronchi Antonella Ellena , Dianzani Irma , Ellis Steven R. , Fresu Luigia Grazia , Aspesi Anna
Published: December 2022

Introduction: Diamond Blackfan anemia (DBA) is a rare congenital disease characterized by defective maturation of the erythroid progenitors in the bone marrow, for which treatment involves steroids, chronic transfusions, or hematopoietic stem cells transplantation. Diamond Blackfan anemia is caused by defective ribosome biogenesis due to heterozygous pathogenic variants in one of 19 ribosomal protein (RP) genes. The decreased number of functional ribosomes leads to the activation of pro-apoptotic pathways and to the reduced translation of key genes for erythropoiesis.

Results and discussion: Here we characterized the phenotype of RPS26-deficiency in a cell line derived from human umbilical cord blood erythroid progenitors (HUDEP-1 cells). This model recapitulates cellular hallmarks of Diamond Blackfan anemia including: imbalanced production of ribosomal RNAs, upregulation of pro-apoptotic genes and reduced viability, and shows increased levels of intracellular calcium. Evaluation of the expression of erythroid markers revealed the impairment of erythroid differentiation in RPS26-silenced cells compared to control cells.

Conclusions: In conclusion, for the first time we assessed the effect of RPS26 deficiency in a human erythroid progenitor cell line and demonstrated that these cells can be used as a scalable model system to study aspects of DBA pathophysiology that have been refractory to detailed investigation because of the paucity of specific cell types affected in this disorder.
Case report: Hemorrhagic fever with renal syndrome presenting as hemophagocytic lymphohistiocytosis
Authors: De Smet Maarten A. J. , Bogaert Simon , Schauwvlieghe Alexander , Dendooven Amélie , Depuydt Pieter , Druwé Patrick
Published: December 2022

Hemophagocytic lymphohistiocytosis may occur in patients with genetic predisposition and in sporadic cases due to malignancy or infection. We describe a 49-year old man with hemorrhagic fever, type 1 respiratory insufficiency and acute kidney injury. Diagnostic work up showed a hyperinflammatory syndrome, hypertriglyceridemia, hemophagocytosis, very high ferritin and significantly elevated sCD25. The findings were compatible with hemophagocytic lymphohistiocytosis based on the HLH-2004 criteria. Serological testing indentified Puumala virus as the causal pathogen. The patient was successfully treated with pulse corticosteroids, intravenous immunoglobins and supportive therapy.
Factors associated with HIV-1 resistance to integrase strand transfer inhibitors in Spain: Implications for dolutegravir-containing regimens
Authors: Gil Horacio , Delgado Elena , Benito Sonia , Moreno-Lorenzo María , Thomson Michael M. ,   the Spanish Group for the Study of Antiretroviral Drug Resistance
Published: December 2022

Integrase strand transfer inhibitor (INSTI)-containing regimens in HIV-1-infected patients have experienced a global increase. Recently, WHO has emphasized the need to fast-track the transition to dolutegravir (DTG)-based antiretroviral (ARV) treatments. However, continued surveillance of INSTI resistance is recommended. In this study, clinical, epidemiological, and virological features associated with INSTI resistance diagnosed in Spain were analyzed. Samples collected between 2008 and 2021 from HIV-1-infected patients were analyzed in integrase, protease, and reverse transcriptase using Sanger population sequencing. ARV drug resistance was evaluated with the Stanford University HIVdb program. Among 2,696 patients, 174 (6.5%) had INSTI resistance, all of them to first-generation INSTIs, and 71 (2.6%) had also resistance to second-generation INSTIs. Of these, only 5 individuals were exposed to DTG as the only INSTI, in whom resistance development was associated with poor treatment adherence and/or resistance to other ARV classes. Of newly HIV-1-diagnosed individuals, 0.92% harbored INSTI-resistant viruses, with low prevalences maintained along time, and only one had low-level resistance to DTG. Persons who inject drugs, age over 39 years, resistance to other ARV classes, and longer time from diagnosis were associated with INSTI resistance (p < 0.001). Non-subtype B INSTI-resistant viruses lacked the Q148H + G140S resistance pathway and showed lower INSTI resistance levels than subtype B viruses. In conclusion, INSTI resistance is uncommon and associated with long-term infections, older age and additional resistance to other ARV drug classes, and is rare in newly diagnosed HIV-1 infections. Our results also support the preferential use of DTG-containing regimens in first-line treatments, although surveillance of INSTI resistance is encouraged.
Identification of DOT1L inhibitor in a screen for factors that promote dopaminergic neuron survival
Authors: Cui Jun , Carey Joseph , Reijo Pera Renee A.
Published: December 2022

Parkinson’s disease (PD) is a common neurodegenerative disorder characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra region of the midbrain. Diagnostic criteria for PD require that at least two of three motor signs are observed: tremor, rigidity, and/or bradykinesia. The most common and effective treatment for PD is Levodopa (L-DOPA) which is readily converted to DA and has been the primary treatment since the 1960’s. Dopamine agonists have also been developed but are less effective than L-DOPA. Although the lack of a model system to study PD has hampered efforts to identify treatments, diverse screening strategies have been proposed for identification of new pharmaceutical candidates. Here, we describe a pilot screen to identify candidate molecules from a bioactive compound library, that might increase formation, maintenance and/or survival of DA neurons in vitro. The screen used a previously characterized reporter construct consisting of the luciferase gene inserted downstream of the endogenous tyrosine hydroxylase (TH) gene and neurons differentiated from human pluripotent stem cells for 18 days. The reporter mimics expression of TH and includes a secreted luciferase whose activity can be measured non-invasively over multiple timepoints. Screening of the bioactive compound library resulted in the identification of a single molecule, SGC0946, that is an inhibitor of DOT1L (Disruptor Of Telomeric silencing 1-Like) which encodes a widely-conserved histone H3K79 methyltransferase that is able to both activate and repress gene transcription. Our results indicate that SGC0946 increased reporter luciferase activity with a single treatment for 48-h post-plating being equivalent to continuous treatment. Moreover, data suggested that the total number of neurons differentiated in the assays was comparable from experiment to experiment under different SGC0946 treatments over time. In contrast, data suggested that the survival and/or maintenance of DA neurons might be specifically enhanced by SGC0946 treatment. These results document the feasibility of a set of tools for further exploration of small molecules that may impact DA neuron differentiation, maintenance and/or survival. Results provide evidence in support of other reports that indicate inhibition of DOT1L may play an important role in maintenance and survival of neural progenitor cells (NPCs) and their lineage-specific differentiation.
Distinct binding pattern of EZH2 and JARID2 on RNAs and DNAs in hepatocellular carcinoma development
Authors: Wen Zhili , He Ke , Zhan Meixiao , Li Yong , Liu Fei , He Xu , Wei Yanli , Zhao Wei , Zhang Yu , Xue Yaqiang , Xia Yong , Wang Fenfen , Xia Zhenglin , Xin Yongjie , Wu Yeye , Duan Xiaopeng , Xiao Jing , Shen Feng , Feng Yuliang , Xiang Guoan , Lu Ligong
Published: December 2022

Hepatocellular carcinoma (HCC) is one of the most malignant cancers worldwide, with high mortality. However, the molecular regulatory mechanisms of liver cancer, especially transcriptional and post-transcriptional mechanisms, should be further studied. Here we used chromatin and cross-linking immunoprecipitation with high throughput sequencing methods (ChIP-seq and CLIP-seq) to capture the global binding profiles on RNAs and DNAs of Enhancer of zeste homolog 2 (EZH2) and its partner Jumonji And AT-Rich Interaction Domain Containing 2 (JARID2) in liver carcinoma cell lines (HepG2) and normal liver cell line (THLE-2), respectively. We also integrated HCC transcriptome data from the TCGA to analyze the expression pattern of bound genes. We found that EZH2 and JARID2 both showed distinct binding profiles between HepG2 and THLE-2 cells. By binding to the primary RNAs, bound transcripts of EZH2 and JARID2 in HepG2 showed significantly increased transcriptional levels in HCC patients. By performing gene set enrichment analysis (GSEA), the bound transcripts were also highly related to HCC development. We also found EZH2 and JARID2 could specifically bind to several long noncoding RNAs (lncRNAs), including H19. By exploring the DNA binding profile, we detected a dramatically repressed DNA binding ability of EZH2 in HepG2 cells. We also found that the EZH2-bound genes showed slightly increased transcriptional levels in HepG2 cells. Integrating analysis of the RNA and DNA binding profiles suggests EZH2 and JARID2 shift their binding ability from DNA to RNA in HepG2 cells to promote cancer development in HCC. Our study provided a comprehensive and distinct binding profile on RNAs and DNAs of EZH2 and JARID2 in liver cancer cell lines, suggesting their potential novel functional manners to promote HCC development.
The detection of prostate cancer based on ultrasound RF signal
Authors: Xiao Tianlei , Shen Weiwei , Wang Qingming , Wu Guoqing , Yu Jinhua , Cui Ligang
Published: December 2022

Objective

The diagnosis of prostate cancer has been a challenging task. Compared with traditional diagnosis methods, the radiofrequency (RF) signal is not only non-invasive but also rich in microscopic lesion information. This paper proposes a novel and accurate method for detecting prostate cancer based on the ultrasound RF signal.

Method

Our approach is based on low-dimensional features in the frequency domain and high-throughput features in the spatial domain. The whole process could be divided into two parts: first, we calculate three feature maps from the ultrasound original RF signal, and 1,050 radiomics features are extracted from the three feature maps; second, we extracted 37 spectral features from the normalized frequency spectrum after Fourier transform.

Results

We use LASSO regression as the method for feature selection; moreover, we use support vector machine (SVM) for classification 10-fold cross-validation for examining the classification performance of the SVM. An AUC (area under the receiver operating characteristic curve) of 0.84 was obtained on 71 subjects.

Conclusions

Our method is feasible to detect prostate cancer based on the ultrasound RF signal with superior classification performance.
The multivariate cox regression model for complete enteral nutrition after primary anastomosis in neonates with intestinal atresia
Authors: Chen Yang , Zhu Le-dao , Zhou Ling , Guan Ai-hui , Wang Zhi-yong , Xiao Dong , Ma Xiao-peng , Ren Feng
Published: December 2022

Objective

Enteral feeding after intestinal atresia has always been a concern for clinicians. But the present studies mainly focused on single factors. This research aimed to comprehensively analyze the multiple factors on complete enteral nutrition after primary anastomosis, and establish the convenient prediction model.

Methods

We retrospectively collected reliable information in neonates with intestinal atresia form January 2010 to June 2022. The cox regression analysis was performed to select independent risk factors and develop nomogram. Subsequently, ROC curve, calibration curve and decision curve were drawn to thoroughly evaluate the accuracy and applicability of the model.

Results

The predictors finally included in the model were gestational age, meconium peritonitis, distance from the anastomosis to the ileocecal region, diameter ratio of proximal to distal bowels, and time of initial feeding. The nomogram of predicting the probability of week 2, week 3 and week 4 was drawn and their area under the curve were 0.765, 0.785 and 0.747, respectively. Similarly, calibration and decision curve indicated that the prediction model had a great prediction performance.

Conclusion

The clinical value of predictive models can be recognized. The hope is that the predictive model can help pediatricians reduce hospital costs and parental anxiety.