bluuebunny/crossref_metadata_2025 huggingface dataset:
Cell-Type Dependent Regulation of the Electrogenic Na+/HCO3− Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma
Authors: Giannaki Marina , Ruf Debora E. , Pfeifer Emilie , Everaerts Katharina , Heiland Dieter H. , Schnell Oliver , Rose Christine R. , Roussa Eleni
Published: August 2022

Glioblastoma multiforme (GBM) is the most common and malignant brain tumour. It is characterised by transcriptionally distinct cell populations. In tumour cells, physiological pH gradients between the intracellular and extracellular compartments are reversed, compared to non-cancer cells. Intracellular pH in tumour cells is alkaline, whereas extracellular pH is acidic. Consequently, the function and/or expression of pH regulating transporters might be altered. Here, we investigated protein expression and regulation of the electrogenic sodium/bicarbonate cotransporter 1 (NBCe1) in mesenchymal (MES)-like hypoxia-dependent and -independent cells, as well as in astrocyte-like glioblastoma cells following chemical hypoxia, acidosis and elucidated putative underlying molecular pathways. Immunoblotting, immunocytochemistry, and intracellular pH recording with the H+-sensitive dye 2′,7′-bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein were applied. The results show NBCe1 protein abundance and active NBCe1 transport. Hypoxia upregulated NBCe1 protein and activity in MES-like hypoxia-dependent GBM cells. This effect was positively correlated with HIF-1α protein levels, was mediated by TGF-β signalling, and was prevented by extracellular acidosis. In MES-like hypoxia-independent GBM cells, acidosis (but not hypoxia) regulated NBCe1 activity in an HIF-1α-independent manner. These results demonstrate a cell-specific adaptation of NBCe1 expression and activity to the microenvironment challenge of hypoxia and acidosis that depends on their transcriptional signature in GBM.
Targeting NLRP3-Mediated Neuroinflammation in Alzheimer’s Disease Treatment
Authors: Barczuk Julia , Siwecka Natalia , Lusa Weronika , Rozpędek-Kamińska Wioletta , Kucharska Ewa , Majsterek Ireneusz
Published: August 2022

Alzheimer’s disease (AD) is the most common cause of dementia in the general population and, to date, constitutes a major therapeutic challenge. In the pathogenesis of AD, aggregates of amyloid β (Aβ) and neurofibrillary tangles (NFTs) containing Tau-microtubule-associated protein (tau) are known to trigger a neuroinflammatory response with subsequent formation of an inflammasome. In particular, the NOD-like receptor pyrin domain-containing 3 (NLRP3) inflammasome is thought to play a crucial role in AD-related pathology. While the mechanisms for NLRP3 activation are not fully understood, it has been demonstrated that, after detection of protein aggregates, NLRP3 induces pro-inflammatory cytokines, such as interleukin 18 (IL-18) or interleukin 1β (IL-1β), that further potentiate AD progression. Specific inhibitors of NLRP3 that exhibit various mechanisms to attenuate the activity of NLRP3 have been tested in in vivo studies and have yielded promising results, as shown by the reduced level of tau and Aβ aggregates and diminished cognitive impairment. Herein, we would like to summarize the current state of knowledge on NLRP3 inflammasome priming, activation, and its actual role in AD pathogenesis, and to characterize the NLRP3 inhibitors that have been studied most and their impact on AD-related pathology.
Agomelatine Changed the Expression and Methylation Status of Inflammatory Genes in Blood and Brain Structures of Male Wistar Rats after Chronic Mild Stress Procedure
Authors: Bialek Katarzyna , Czarny Piotr , Wigner Paulina , Synowiec Ewelina , Kolodziej Lukasz , Bijak Michal , Szemraj Janusz , Papp Mariusz , Sliwinski Tomasz
Published: August 2022

The preclinical research conducted so far suggest that depression development may be influenced by the inflammatory pathways both at the periphery and within the central nervous system. Furthermore, inflammation is considered to be strongly connected with antidepressant treatment resistance. Thus, this study explores whether the chronic mild stress (CMS) procedure and agomelatine treatment induce changes in TGFA, TGFB, IRF1, PTGS2 and IKBKB expression and methylation status in peripheral blood mononuclear cells (PBMCs) and in the brain structures of rats. Adult male Wistar rats were subjected to the CMS and further divided into matched subgroups to receive vehicle or agomelatine. TaqMan gene expression assay and methylation-sensitive high-resolution melting (MS-HRM) were used to evaluate the expression of the genes and the methylation status of their promoters, respectively. Our findings confirm that both CMS and antidepressant agomelatine treatment influenced the expression level and methylation status of the promoter region of investigated genes in PBMCs and the brain. What is more, the present study showed that response to either stress stimuli or agomelatine differed between brain structures. Concluding, our results indicate that TGFA, TGFB, PTGS2, IRF1 and IKBKB could be associated with depression and its treatment.
Can Leukotriene Receptor Antagonist Therapy Improve the Control of Patients with Severe Asthma on Biological Therapy and Coexisting Bronchiectasis? A Pilot Study
Authors: Quaranta Vitaliano , Dragonieri Silvano , Crimi Nunzio , Crimi Claudia , Santus Pierachille , Menzella Francesco , Pelaia Corrado , Scioscia Giulia , Caruso Cristiano , Bargagli Elena , Kostikas Konstantinos , Kyriakopoulos Christos , Scichilone Nicola , Carpagnano Giovanna
Published: August 2022

Introduction: Asthma and bronchiectasis appear to be two related diseases and in their complex inflammatory interaction, the cysteinyl leukotriene/cysteinyl leukotriene receptor 1 (cysLT/cysLTR1) axis appears to play an important role given its involvement also in the neutrophilic pathway. To our knowledge, few studies have been conducted so far to evaluate the role of the leukotriene cysLT/cysLTr1 axis in the management of clinical and inflammatory outcomes within a population of patients with severe asthma and bronchiectasis. The aim of our study was to verify in this population the effect of leukotriene receptor antagonist (LTRA) therapy in clinical and inflammatory control before and after 6 months of introduction of biologic therapy. Methods: We retrospectively enrolled, from eight different severe asthma centers’ outpatients, 36 atopic patients with the simultaneous presence of non-cystic fibrosis (non-CF) and non-allergic bronchopulmonary aspergillosis (non-ABPA) bronchiectasis and severe asthma. The first biological injection was performed at baseline (T0 time). Patients who were already taking LTRA therapy at time T0 were recorded, and no new prescriptions were made. We observed our population over a 6-month period (T1 time). At the baseline we collected the following data: baseline characteristics, clinical history, high resolution computed tomography and bronchiectasis-related parameters and skin prick test. At both times T0 and T1 we collected the following data: asthma control test (ACT), asthma control questionnaire (ACQ), immunoglobulin E (IgE) level, blood count, fractional exhaled nitric oxide 50 (FeNO 50) and flow-volume spirometry. The study was retrospectively registered. Results: Our population had a mean age of 59.08 ± 11.09 and 50% were female. At T1, patients on LTRA therapy had a significantly lower FeNO value (33.03 ± 23.61 vs. 88.92 ± 77.96; p = 0.012). We assessed that the value of ΔFeNO (FeNO 50 T1 − FeNO 50 T0) and the number of unplanned specialist visits allowed a discrimination of 66.7% in the presence of LTRA therapy. We also verified how low FeNO values at time T1 were statistically significant predictors of LTRA therapy (ODD = 9.96 (0.94–0.99); p = 0.032). Conclusion: The presence of LTRA in therapy in a population of severe asthmatics with coexisting non-ASBPA bronchiectasis and non-cystic fibrosis, acting simultaneously on the T helper type 2 (TH2) pathway and probably on the neutrophilic component of bronchiectasis, would allow a further amplification of the beneficial effects of biological therapy, leading to a reduction in the number of unplanned visits to specialists.
Takotsubo Syndrome: From Bench to Bedside and Bedside to Bench
Authors: Pelliccia Francesco , Morgantini Amalia , Rosati Riccardo
Published: August 2022

Takotsubo syndrome (TTS) typically manifests as acute chest pain and/or dyspnea triggered by intense psychological or physiological stress [...]
Influence of Ultrafine-Grained Microstructure and Texture Evolution of ECAPed ZK30 Magnesium Alloy on the Corrosion Behavior in Different Corrosive Agents
Authors: Alateyah Abdulrahman I. , Alawad Majed O. , Aljohani Talal A. , El-Garaihy Waleed H.
Published: August 2022

Magnesium-Zinc-Zirconium (Mg-Zn-Zr) alloys have caught considerable attention in medical applications where biodegradability is critical. The combination of their good biocompatibility, improved strength, and low cytotoxicity makes them great candidates for medical implants. This research investigation is focused on providing further insight into the effects of equal channel angular processing (ECAP) on the corrosion behavior, microstructure evolution, and mechanical properties of a biodegradable ZK30 alloy. Billets of Mg-3Zn-0.6 Zr (ZK30) alloy were processed through ECAP up to 4 passes of route Bc (rotating the billets 90° in the same direction between the subsequent passes) at 250 °C. Electron back-scatter diffraction (EBSD) was utilized to investigate the microstructural evolution as well as the crystallographic texture. Several electrochemical measurements were carried out on both a simulated body fluid and a 3.5% sodium chloride (NaCl) solution. Mechanical properties such as Vicker’s hardness and tensile properties were also assessed. The as-annealed (AA) microstructure was dominated by equiaxed coarse recrystallized grains with an average grain size of 26.69 µm. After processing, a geometric grain subdivision took place due to the severe plastic deformation. Processed samples were characterized by grain refinement and high density of substructures. The 4-passes sample experienced a reduction in the grain size by 92.8% compared with its AA counterpart. The fraction of high-angle grain boundaries increased significantly after 4-passes compared to the 1-pass processed sample. With regards to the crystallographic texture, the AA condition had its {0001} basal planes mostly oriented parallel to the transversal direction. On the other hand, ECAP processing resulted in crystallographic texture changes, such as the shifting of the ZK30 shear plane to be aligned at 45° relative to the extrusion direction (ED). Furthermore, the maximum texture intensity was reduced from 14 times random (AA billets) to 8 times random after ECAP processing through 4-passes. The corrosion rate of the 4-passes sample was tremendously reduced by 99% and 45.25% compared with its AA counterpart in the simulated body fluid and the NaCl solution, respectively. The pitting corrosion resistance of ZK30 showed notable improvements in the simulated body fluid by 471.66% and 352% during processing through 1-pass and 4-passes, respectively, compared with the 3.5% NaCl findings. Finally, significant improvements in the tensile strength, hardness, and ductility were also achieved.
Overview of Concrete Performance Made with Waste Rubber Tires: A Step toward Sustainable Concrete
Authors: Ahmad Jawad , Zhou Zhiguang , Majdi Ali , Alqurashi Muwaffaq , Deifalla Ahmed Farouk
Published: August 2022

Utilizing scrap tire rubber by incorporating it into concrete is a valuable option. Many researchers are interested in using rubber tire waste in concrete. The possible uses of rubber tires in concrete, however, are dispersed and unclear. Therefore, a compressive analysis is necessary to identify the benefits and drawbacks of rubber tires for concrete performance. For examination, the important areas of concrete freshness, durability, and strength properties were considered. Additionally, several treatments and a microstructure investigation were included. Although it has much promise, there are certain obstacles that prevent it from being used as an aggregate in large numbers, such as the rubber’s weak structural strength and poor binding performance with the cement matrix. Rubber, however, exhibits mechanical strength comparable to reference concrete up to 20%. The evaluation also emphasizes the need for new research to advance rubberized concrete for future generations.
Electrochemical and Mechanical Properties of Cathodically Protected X80 Steel in Different Temperature Soil
Authors: Liu Wenhui , Meng Yanbing , Zhao Jun , Wen Wen , Gong Ming , Wu Shixiong , Li Songmei , Yu Mei , Liu Jianhua
Published: August 2022

For the application of X80 pipelines in Northeast China, it is important to establish the correct cathodic protection (CP) potential. To achieve this, potentiodynamic polarization; electrochemical impedance spectroscopy (EIS); a slow strain rate test (SSRT); and a scanning electron microscopy (SEM) fracture morphology analysis were carried out for an X80 steel gas pipeline at several temperatures in Heilongjiang Province, China. The results show that the hydrogen evolution potential of X80 steel in soil at different temperatures was about −900 mV (vs. CSE). The generated hydrogen atoms can be adsorbed on the surface of the pipelines to reduce the surface energy, or they can be diffused into the substrate and accumulate to the critical concentration, inducing the decohesion between different structures and generating additional plastic deformation through dislocation motion. With the peak impedance potential as the minimum potential and the hydrogen embrittlement potential as the maximum potential, the CP potential of X80 steel in the soil at 30 °C, 45 °C, and 60 °C ranged from −900 mV to −1100 mV (vs. CSE), temperatures at which the X80 steel does not corrode or cause hydrogen embrittlement.
Chemical, Radiometric and Mechanical Characterization of Commercial Polymeric Films for Greenhouse Applications
Authors: Franco John Eloy , Rodríguez-Arroyo Jesús Antonio , Ortiz Isabel María , Sánchez-Soto Pedro José , Garzón Eduardo , Lao María Teresa
Published: August 2022

In the agricultural sector, companies involved in the production of plastic greenhouses are currently searching for a suitable covering adapted for every climate in the world. For this purpose, this research work has determined the chemical, radiometric and mechanical properties of 53 polymeric films samples from Europe and South America. The chemical tests carried out with these samples were elemental analysis (C, H and N) and FT–IR spectrometry. The radiometric properties here studied were the transmission, absorption and reflection coefficients along the spectrum between 300 and 1100 nm. For the mechanical properties, tensile strength, tear strength and dart impact strength, tests were carried out. Finally, all these data were collected, and a multivariate statistical analysis was carried out using the SPSS statistical to group the samples into statistical groups adapted to specific climatic regions. The elemental analysis and FT–IR spectrometry allowed group the samples into nine groups. The samples were grouped according to their chemical (elemental analysis), radiometric and mechanical properties by multivariate analysis. The dendrogram separated five very different groups in terms of number of samples. These groups have specific chemical, radiometric and mechanical characteristics that separate them from the rest. These groups make it possible to narrow down the applications and correlate with the radiometric properties to see in which geographical area of the world they are most effective in increasing yields and achieving higher quality production.
Degradation of CdS Yellow and Orange Pigments: A Preventive Characterization of the Process through Pump–Probe, Reflectance, X-ray Diffraction, and Raman Spectroscopy
Authors: Assunta Pisu Francesca Assunta , Ricci Pier Carlo , Porcu Stefania , Carbonaro Carlo Maria , Chiriu Daniele
Published: August 2022

Cadmium yellow degradation afflicts numerous paintings realized between the XIXth and XXth centuries. The degradation process and its kinetics is not completely understood. It consists of chalking, lightening, flaking, spalling, and, in its most deteriorated cases, the formation of a crust over the original yellow paint. In order to improve the comprehension of the process, mock-up samples of CdS in yellow and orange tonalities were studied by means of structural analysis and optical characterization, with the principal techniques used in the field of cultural heritage. Mock ups were artificially degraded with heat treatment and UV exposure. Relevant colorimetric variation appears in CIE Lab coordinates from reflectance spectra. XRD, SEM-EDS, and Raman spectroscopy revealed the formation of cadmium sulfate, whilst time-resolved photoluminescence and pump–probe transient absorption spectroscopy suggest the formation of a defective phase, compatible with Cd vacancies and the formation of both CdO and CdSO4 superficial clusters.
Large-Area Fabrication of LIPSS for Wetting Control Using Multi-Parallel Femtosecond Laser Processing
Authors: Indrišiūnas Simonas , Svirplys Evaldas , Gedvilas Mindaugas
Published: August 2022

In this research, the wetting property control of a stainless-steel surface, structured using parallel processing via an array of 64-femtosecond laser beams, is presented. The scanning of an 8 × 8-beam array over the sample was used to uniformly cover the large areas with LIPSS. The static water contact angle and the LIPSS period dependence on processing parameters were investigated. The wettability control of water droplets on laser-patterned stainless steel, ranging from contact angles of ~63°, similar to those of the plain surface, to the superhydrophobic surface with contact angles > 150°, was achieved. The relationship between the static water contact angle and the LIPSS parameters in the Fourier plane was investigated.
High-Purity Fucoxanthin Can Be Efficiently Prepared from Isochrysis zhangjiangensis by Ethanol-Based Green Method Coupled with Octadecylsilyl (ODS) Column Chromatography
Authors: Zhuang Gengjie , Ye Yuemei , Zhao Junling , Zhou Chengxu , Zhu Junwang , Li Yanrong , Zhang Jinrong , Yan Xiaojun
Published: August 2022

The exploitation of new economically valuable microalgae as a sustainable source of minor high-value products can effectively promote the full utilization of microalgae. The efficient preparation of minor products from microalgae remains the challenge, owing to the coexistence of various components with a similar polarity in the microalgae biomass. In this study, a novel approach based on the sustainable-oriented strategy for fucoxanthin (FX) production was proposed, which consisted of four steps, including the culture of microalga, ethanol extraction, ODS column chromatography, and ethanol precipitation. The high-purity FX (around 95%) was efficiently obtained in a total recovery efficiency of 84.28 ± 2.56%. This study reveals that I. zhangjiangensis is a potentially promising feedstock for FX production and firstly provides a potentially eco-friendly method for the scale-up preparation of FX from the microalga I. zhangjiangensis.
A Portable Continuous-Flow Polymerase Chain Reaction Chip Device Integrated with Arduino Boards for Detecting Colla corii asini
Authors: Sheu Shyang-Chwen , Song Yi-Syuan , Chen Jyh-Jian
Published: August 2022

Food security is a significant issue in modern society. Because morphological characters are not reliable enough to distinguish authentic traditional Chinese medicines, it is essential to establish an effective and applicable method to identify them to protect people’s health. Due to the expensive cost of the manufacturing process and the large volume of the analytical system, the need to build a portable and cheap device is urgent. This work describes the development of a portable nucleic acid amplification device integrated with thermal control and liquid pumping connecting to Arduino boards. We present a novel microfluidic polymerase chain reaction (PCR) chip with symmetric isothermal zones. The total chip volume is small, and only one Arduino board is needed for thermal control. We assemble a miniaturized liquid pump and program an Arduino file to push the sample mixture into the chip to implement the PCR process. In the proposed operation, the Nusselt number of the sample flow is less than one, and the heat transfer is conduction only. Then we can ensure temperature uniformity in specific reaction regions. A Colla corii asini DNA segment of 200 bp is amplified to evaluate the PCR performance under the various operational parameters. The initial concentration for accomplishing the PCR process is at least 20 ng/μL at the flow rate of 0.4 μL/min in the portable continuous flow PCR (CFPCR) device. To our knowledge, our group is the first to introduce Arduino boards into the heat control and sample pumping modules for a CFPCR device.
Thermal Transmission Comparison of Nanofluids over Stretching Surface under the Influence of Magnetic Field
Authors: Arshad Mubashar , Karamti Hanen , Awrejcewicz Jan , Grzelczyk Dariusz , Galal Ahmed M.
Published: August 2022

Heat transfer at industrial levels has been revolutionized with the advancement of nanofluid and hybrid nanofluid. Keeping this development in view, this article aims to present the rate of heat transfer for conventional and hybrid nanofluids, incorporating the Hall Effect over a stretchable surface. The flow governing equations are obtained with the help of suitable assumptions, and the problem is attempted with the boundary value problem technique in MATLAB. The highly non-linear partial differential equations are transformed into non-dimensional forms using suitable similarity transforms. The criterion of convergence for solution or tolerance of a problem is adjusted to 10−7. Water is considered as a base fluid; copper (Cu) and silver (Ag) nanoparticles are mixed to obtain nanofluid. This novel work is incorporated for conventional and hybrid nanofluid with the effect of Hall current above the stretching/shrinking surface. Increasing the Stefan blowing parameter reduces the flow rate; it increases the heat transfer rate and nano-particle concentration of conventional and hybrid nanofluid. Both velocity components decreases by increasing the magnetic field. The Hall Effect also decreases the velocity of nanofluid. The outcomes are compared to previously published work, demonstrating that the existing study is legitimate. The heat transfer rate of the hybrid nanofluid is higher than the convential nanofluid. This study suggests more frequent use of hybrid nanofluid because of high heat transfer rates and reduced skin friction.
The Impact of Detrital Minerals on Reservoir Flow Zones in the Northeastern Bredasdorp Basin, South Africa, Using Core Data
Authors: Opuwari Mimonitu , Ubong Moses Okon , Jamjam Simamkele , Magoba Moses
Published: August 2022

The present study uses core data to group reservoirs of a gas field in the Bredasdorp Basin offshore South Africa into flow zones. One hundred and sixty-eight core porosity and permeability data were used to establish reservoir zones from the flow zone indicator (FZI) and Winland’s methods. Storage and flow capacities were determined from the stratigraphy-modified Lorenz plot (SMLP) method. The effects of the mineralogy on the flow zones were established from mineralogy composition analyses using quantitative X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Results reveal five flow zones grouped as high, moderate, low, very low, and tight reservoir rocks. The high flow zone is the best reservoir quality rock and has porosity and permeability values ranging from 12 to 20% and 100 to 1000 mD. The high and moderate zones contribute more than 60% of each well’s flow capacities. The moderate and low flow zone extends laterally to all the wells. The tight flow zone is an impervious rock and has the lowest rock quality with porosity and permeability values less than 8% and 1 mD, respectively. This zone contributes less than 1% to flow capacity. The impact of minerals on flow zones is evident in plagioclase and muscovite content increases. An accompanied decrease in quartz content is observed, which implies that low plagioclase content ≤4% and muscovite content of ≤1% corresponds to the low, moderate, and high flow zones, while plagioclase content of ≥4% and muscovite content of ≥1% belong to the tight flow zone. Consequently, the quantity of plagioclase and muscovite can be used as a proxy to identify better quality reservoir rocks. The diagenetic process that reduces the rock quality can be attributed to quartz overgrowth and the accumulation of mica flakes in the pore spaces. In contrast, the fracture in the high flow zone is the reservoir quality enhancing process. The flow zones are generally controlled by a combination of facies and diagenetic factors.
Tunnel Structure Enhanced Polysulfide Conversion for Inhibiting “Shuttle Effect” in Lithium-Sulfur Battery
Authors: Guo Xiaotong , Bi Xu , Zhao Junfeng , Yu Xinxiang , Dai Han
Published: August 2022

The Lithium sulfur (Li-S) battery has a great potential to replace lithium-ion batteries due to its high-energy density. However, the “shuttle effect” of polysulfide intermediates (Li2S8, Li2S6, Li2S4, etc.) from the cathode can lead to rapid capacity decay and low coulombic efficiency, thus limiting its further development. Anchoring polysulfide and inhibiting polysulfide migration in electrolytes is one of the focuses in Li-S battery. It is well known that polar metal oxides-manganese oxides (MnO2) are normally used as an effective inhibitor for its polysulfide inhibiting properties. Considering the natural 1D tunnel structure, MnO2 with three kinds of typical tunnel-type were screened to study the effects of the tunnel size on the adsorption capacity of polysulfide. We found that MnO2 with larger tunnel sizes has stronger chemisorption capacity of polysulfide. It promotes the conversion of polysulfide, and corresponding cathode exhibits better cycle reliability and rate performance in the cell comparison tests. This work should point out a new strategy for the cathode design of advanced Li-S battery by controlling the tunnel size.
Evolution in Lithography Techniques: Microlithography to Nanolithography
Authors: Sharma Ekta , Rathi Reena , Misharwal Jaya , Sinhmar Bhavya , Kumari Suman , Dalal Jasvir , Kumar Anand
Published: August 2022

In this era, electronic devices such as mobile phones, computers, laptops, sensors, and many more have become a necessity in healthcare, for a pleasant lifestyle, and for carrying out tasks quickly and easily. Different types of temperature sensors, biosensors, photosensors, etc., have been developed to meet the necessities of people. All these devices have chips inside them fabricated using diodes, transistors, logic gates, and ICs. The patterning of the substrate which is used for the further development of these devices is done with the help of a technique known as lithography. In the present work, we have carried out a review on different types of lithographic techniques such as optical lithography, extreme ultraviolet lithography, electron beam lithography, X-ray lithography, and ion beam lithography. The evolution of these techniques with time and their application in device fabrication are discussed. The different exposure tools developed in the past decade to enhance the resolution of these devices are also discussed. Chemically amplified and non-chemically amplified resists with their bonding and thickness are discussed. Mask and maskless lithography techniques are discussed along with their merits and demerits. Device fabrication at micro and nano scale has been discussed. Advancements that can be made to improve the performance of these techniques are also suggested.
Studies of the Structure and Optical Properties of BaSrMgWO6 Thin Films Deposited by a Spin-Coating Method
Authors: Punga Luciana , Abbassi Abderrahman , Toma Mihaela , Alupului Teodor , Doroftei Corneliu , Dobromir Marius , Timpu Daniel , Doroftei Florica , Hrostea Laura , Rusu George G. , Razouk Abdelati , Iacomi Felicia
Published: August 2022

Highly transparent thin films with the chemical formula BaSrMgWO6 were deposited by spin coating using a solution of nitrates of Ba, Sr, and Mg and ammonium paratungstate in dimethylformamide with a Ba:Sr:Mg:W ratio = 1:1:1:1. XRD, SEM, EDX, and XPS investigations evidenced that annealing at 800 °C for 1 h results in an amorphous structure having a precipitate on its surface, and that supplementary annealing at 850 °C for 45 min forms a nanocrystalline structure and dissolves a portion of the precipitates. A textured double perovskite cubic structure (61.9%) was found, decorated with tetragonal and cubic impurity phases (12.7%), such as BaO2, SrO2, and MgO, and an under-stoichiometric phase (24.4%) with the chemical formula Ba2−(x+y) SrxMgyWO5. From transmittance measurements, the values of the optical band gap were estimated for the amorphous (Egdir = 5.21 eV, Egind = 3.85 eV) and nanocrystalline (Egdir = 4.69 eV, Egind = 3.77 eV) phases. The presence of a lattice disorder was indicated by the high Urbach energy values and weak absorption tail energies. A decrease in their values was observed and attributed to the crystallization process, lattice strain diminution, and cation redistribution.
Public Awareness of Diet-Related Diseases and Dietary Risk Factors: A 2022 Nationwide Cross-Sectional Survey among Adults in Poland
Authors: Żarnowski Adam , Jankowski Mateusz , Gujski Mariusz
Published: August 2022

A suboptimal diet is a risk factor for numerous non-communicable diseases. This study aimed to assess the level of knowledge on diet-related diseases and dietary risk factors among adults in Poland as well as to identify factors associated with awareness of diet-related diseases and dietary risk factors. This cross-sectional survey was carried out in July 2022 on a representative sample of adults in Poland. Data were received from 1070 individuals (53.3% females) aged 18–89 years. Out of eight diet-related diseases included in this study, overweight/obesity was the most recognized diet-related disease (85.0%). Stroke (26.2%) and osteoporosis (17.9%) were the least recognized diet-related diseases. Out of eight dietary risk factors included in this study, excessive consumption of sugar and salt (73.4%) was the most recognized dietary risk factor. Less than half of the respondents were aware that (1) too little vitamin intake, (2) too little intake of calcium and magnesium, (3) too little consumption of fish and oils, and (4) too little dietary fiber intake can lead to the development of the diseases. Having higher education and the presence of chronic diseases were the most important factors associated with a higher level of awareness of diet-related diseases and dietary risk factors (p < 0.05).
Serum Metabolomics and Proteomics to Study the Antihypertensive Effect of Protein Extracts from Tenebrio molitor
Authors: Stella Roberto , Peggion Caterina , Bergantin Caterina , Biancotto Giancarlo , Frosini Maria , Dreassi Elena , Marcolongo Paola , Aloisi Anna Maria , Pessina Federica
Published: August 2022

Hypertension is the leading risk factor for premature death worldwide and significantly contributes to the development of all major cardiovascular disease events. The management of high blood pressure includes lifestyle changes and treatment with antihypertensive drugs. Recently, it was demonstrated that a diet supplemented with Tenebrio molitor (TM) extracts is useful in the management of numerous pathologies, including hypertension. This study is aimed at unveiling the underlying mechanism and the molecular targets of intervention of TM dietary supplementation in hypertension treatment by means of proteomics and metabolomics techniques based on liquid chromatography coupled with high-resolution mass spectrometry. We demonstrate that serum proteome and metabolome of spontaneously hypertensive rats are severely altered with respect to their normotensive counterparts. Additionally, our results reveal that a diet enriched with TM extracts restores the expression of 15 metabolites and 17 proteins mainly involved in biological pathways associated with blood pressure maintenance, such as the renin–angiotensin and kallikrein–kinin systems, serin protease inhibitors, reactive oxygen scavenging, and lipid peroxidation. This study provides novel insights into the molecular pathways that may underlie the beneficial effects of TM, thus corroborating that TM could be proposed as a helpful functional food supplement in the treatment of hypertension.
Anti-Inflammatory Diet Prevents Subclinical Colonic Inflammation and Alters Metabolomic Profile of Ulcerative Colitis Patients in Clinical Remission
Authors: Keshteli Ammar , Valcheva Rosica , Nickurak Cheryl , Park Heekuk , Mandal Rupasri , van Diepen Kendall , Kroeker Karen , van Zanten Sander , Halloran Brendan , Wishart David , Madsen Karen , Dieleman Levinus
Published: August 2022

A relationship between ulcerative colitis (UC) and diet has been shown in epidemiological and experimental studies. In a 6-month, open-label, randomized, placebo-controlled trial, adult UC patients in clinical remission were randomized to either an “Anti-inflammatory Diet (AID)” or “Canada’s Food Guide (CFG)”. Menu plans in the AID were designed to increase the dietary intake of dietary fiber, probiotics, antioxidants, and omega-3 fatty acids and to decrease the intake of red meat, processed meat, and added sugar. Stool was collected for fecal calprotectin (FCP) and microbial analysis. Metabolomic analysis was performed on urine, serum, and stool samples at the baseline and study endpoint. In this study, 53 patients were randomized. Five (19.2%) patients in the AID and 8 (29.6%) patients in the CFG experienced a clinical relapse. The subclinical response to the intervention (defined as FCP < 150 µg/g at the endpoint) was significantly higher in the AID group (69.2 vs. 37.0%, p = 0.02). The patients in the AID group had an increased intake of zinc, phosphorus, selenium, yogurt, and seafood versus the control group. Adherence to the AID was associated with significant changes in the metabolome, with decreased fecal acetone and xanthine levels along with increased fecal taurine and urinary carnosine and p-hydroxybenzoic acid levels. The AID subjects also had increases in fecal Bifidobacteriaceae, Lachnospiraceae, and Ruminococcaceae. In this study, we found thatdietary modifications involving the increased intake of anti-inflammatory foods combined with a decreased intake of pro-inflammatory foods were associated with metabolic and microbial changes in UC patients in clinical remission and were effective in preventing subclinical inflammation.
Role of Drug Transporters in Elucidating Inter-Individual Variability in Pediatric Chemotherapy-Related Toxicities and Response
Authors: Kamath Ashwin , Srinivasamurthy Suresh Kumar , Chowta Mukta N. , Ullal Sheetal D. , Daali Youssef , Chakradhara Rao Uppugunduri S.
Published: August 2022

Pediatric cancer treatment has evolved significantly in recent decades. The implementation of risk stratification strategies and the selection of evidence-based chemotherapy combinations have improved survival outcomes. However, there is large interindividual variability in terms of chemotherapy-related toxicities and, sometimes, the response among this population. This variability is partly attributed to the functional variability of drug-metabolizing enzymes (DME) and drug transporters (DTS) involved in the process of absorption, distribution, metabolism and excretion (ADME). The DTS, being ubiquitous, affects drug disposition across membranes and has relevance in determining chemotherapy response in pediatric cancer patients. Among the factors affecting DTS function, ontogeny or maturation is important in the pediatric population. In this narrative review, we describe the role of drug uptake/efflux transporters in defining pediatric chemotherapy-treatment-related toxicities and responses. Developmental differences in DTS and the consequent implications are also briefly discussed for the most commonly used chemotherapeutic drugs in the pediatric population.
Pharmacovigilance of Herb-Drug Interactions: A Pharmacokinetic Study on the Combined Administration of Tripterygium Glycosides Tablets and Leflunomide Tablets in Rats by LC-MS/MS
Authors: Boucetta Hamza , Wu Wei , Hong Tao , Cheng Rui , Jiang Jing , Liu Chengxi , Song Min , Hang Taijun
Published: August 2022

A popular and widely used combination therapy of leflunomide (LEF) and Tripterygium glycosides tablets (TGTS) has become a valuable clinical tool in China for the treatment of rheumatoid arthritis. This regimen has not been evaluated either in terms of interaction or toxicity, even given the rising concerns about LEF’s prolonged elimination half-life and TGT’s narrow therapeutic index, in addition to the current trend of using high doses of LEF. Thus, this study determines the potential adverse drug reactions between these two medicines. Reliable validated LC-MS/MS methods were used for the determination of teriflunomide (TER, the only active metabolite of LEF), and the main components of TGT: wilforlide A, wilforgine, wilfortrine, wilfordine, and wilforine. The results obtained from this investigation, as paralleled with the control groups, revealed that the Cmax and AUC0-t of TER were significantly decreased with separate co-administration, as the Cmax and AUC0-t were 30.17 ± 1.55 μg/mL and 24.47 ± 2.50 μg/mL, 374.55 ± 15.54 μg h/mL and 336.94 ± 21.19 μg h/mL, respectively (p < 0.05). Meanwhile, the pharmacokinetic profiles of the main components of TGT have also been affected by separate co-administration in rats. Therefore, herb–drug interactions between LEF and TGT have been proven.
Protein-Based Adjuvants for Vaccines as Immunomodulators of the Innate and Adaptive Immune Response: Current Knowledge, Challenges, and Future Opportunities
Authors: Díaz-Dinamarca Diego A. , Salazar Michelle L. , Castillo Byron N. , Manubens Augusto , Vasquez Abel E. , Salazar Fabián , Becker María Inés
Published: August 2022

New-generation vaccines, formulated with subunits or nucleic acids, are less immunogenic than classical vaccines formulated with live-attenuated or inactivated pathogens. This difference has led to an intensified search for additional potent vaccine adjuvants that meet safety and efficacy criteria and confer long-term protection. This review provides an overview of protein-based adjuvants (PBAs) obtained from different organisms, including bacteria, mollusks, plants, and humans. Notably, despite structural differences, all PBAs show significant immunostimulatory properties, eliciting B-cell- and T-cell-mediated immune responses to administered antigens, providing advantages over many currently adopted adjuvant approaches. Furthermore, PBAs are natural biocompatible and biodegradable substances that induce minimal reactogenicity and toxicity and interact with innate immune receptors, enhancing their endocytosis and modulating subsequent adaptive immune responses. We propose that PBAs can contribute to the development of vaccines against complex pathogens, including intracellular pathogens such as Mycobacterium tuberculosis, those with complex life cycles such as Plasmodium falciparum, those that induce host immune dysfunction such as HIV, those that target immunocompromised individuals such as fungi, those with a latent disease phase such as Herpes, those that are antigenically variable such as SARS-CoV-2 and those that undergo continuous evolution, to reduce the likelihood of outbreaks.
Effects of Palmitoylethanolamide (PEA) on Nociceptive, Musculoskeletal and Neuropathic Pain: Systematic Review and Meta-Analysis of Clinical Evidence
Authors: Scuteri Damiana , Guida Francesca , Boccella Serena , Palazzo Enza , Maione Sabatino , Rodríguez-Landa Juan Francisco , Martínez-Mota Lucia , Tonin Paolo , Bagetta Giacinto , Corasaniti Maria Tiziana
Published: August 2022

Some 30–50% of the global population and almost 20% of the European population actually suffer from chronic pain, which presents a tremendous burden to society when this pain turns into a disability and hospitalization. Palmitoylethanolamide (PEA) has been demonstrated to improve pain in preclinical contexts, but an appraisal of clinical evidence is still lacking. The present study aimed at addressing the working hypothesis for the efficacy of PEA for nociceptive musculoskeletal and neuropathic pain in the clinical setting. The systematic search, selection and analysis were performed in agreement with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 recommendations. The primary outcome was pain reduction, as measured by a pain assessment scale. The secondary outcome was improvement in quality of life and/or of parameters of function. The results obtained for a total of 933 patients demonstrate the efficacy of PEA over the control (p < 0.00001), in particular in six studies apart from the two randomized, double-blind clinical trials included. However, the results are downgraded due to the high heterogeneity of the studies (I2 = 99%), and the funnel plot suggests publication bias. Efficacy in achieving a reduction in the need for rescue medications and improvement in functioning, neuropathic symptoms and quality of life are reported. Therefore, adequately powered randomized, double-blind clinical trials are needed to deepen the domains of efficacy of add-on therapy with PEA for chronic pain. PROSPERO registration: CRD42022314395.
Cinnamic Acid Attenuates Peripheral and Hypothalamic Inflammation in High-Fat Diet-Induced Obese Mice
Authors: Lee Aden Geonhee , Kang Sora , Im Suyeol , Pak Youngmi Kim
Published: August 2022

Obesity is closely linked to chronic inflammation in peripheral organs and the hypothalamus. Chronic consumption of a high-fat diet (HFD) induces the differentiation of Ly6chigh monocytes into macrophages in adipose tissue, the liver, and the brain, as well as the secretion of pro-inflammatory cytokines. Although cinnamon improves obesity and related diseases, it is unclear which components of cinnamon can affect macrophages and inflammatory cytokines. We performed in silico analyses using ADME, drug-likeness, and molecular docking simulations to predict the active compounds of cinnamon. Among the 82 active compounds of cinnamon, cinnamic acid (CA) showed the highest score of ADME, blood–brain barrier permeability, drug-likeness, and cytokine binding. We then investigated whether CA modulates obesity-induced metabolic profiles and macrophage-related inflammatory responses in HFD-fed mice. While HFD feeding induced obesity, CA ameliorated obesity and related symptoms, such as epididymal fat gain, insulin resistance, glucose intolerance, and dyslipidemia, without hepatic and renal toxicity. CA also improved HFD-induced tumor necrosis factor-α, fat deposition, and macrophage infiltration in the liver and adipose tissue. CA decreased Ly6chigh monocytes, adipose tissue M1 macrophages, and hypothalamic microglial activation. These results suggest that CA attenuates the peripheral and hypothalamic inflammatory monocytes/macrophage system and treats obesity-related metabolic disorders.
A 3D-Printed Sole Design Bioinspired by Cat Paw Pad and Triply Periodic Minimal Surface for Improving Paratrooper Landing Protection
Authors: Xiao Yilin , Hu Dayong , Zhang Zhiqiang , Pei Baoqing , Wu Xueqing , Lin Peng
Published: August 2022

Paratroopers are highly susceptible to lower extremity impact injuries during landing. To reduce the ground reaction force (GRF), inspired by the cat paw pad and triply periodic minimal surface (TPMS), a novel type of bionic cushion sole for paratrooper boots was designed and fabricated by additive manufacturing. A shear thickening fluid (STF) was used to mimic the unique adipose tissue with viscoelastic behavior found in cat paw pads, which is formed by a dermal layer encompassing a subcutaneous layer and acts as the primary energy dissipation mechanism for attenuating ground impact. Based on uniaxial compression tests using four typical types of cubic TPMS specimens, TPMSs with Gyroid and Diamond topologies were chosen to fill the midsole. The quasi-static and dynamic mechanical behaviors of the bionic sole were investigated by quasi-static compression tests and drop hammer tests, respectively. Then, drop landing tests at heights of 40 cm and 80 cm were performed on five kinds of soles to assess the cushioning capacity and compare them with standard paratrooper boots and sports shoes. The results showed that sports shoes had the highest cushioning capacity at a height of 40 cm, whereas at a height of 80 cm, the sole with a 1.5 mm thick Gyroid configuration and STF filling could reduce the maximum peak GRF by 15.5% when compared to standard paratrooper boots. The present work has implications for the design of novel bioinspired soles for reducing impact force.
Catholic Higher Education Globally: Enrollment Trends, Current Pressures, Student Choice, and the Potential of Service Learning
Authors: Wodon Quentin
Published: August 2022

Globally, Catholic universities operate in an increasingly competitive market. They tend to have a strong academic reputation in many countries and their graduates tend to do well professionally. One explanation for this success could be that many universities are selective: they may attract motivated students. In addition, it is sometimes suggested that the values emphasized in Catholic education may also play a role, including for the choice of students to attend a particular university. Whether values and the Catholic faith matter for students, or rather how much they matter, is important for the future of Catholic universities in a context of rising competition as well as pluralism, including in terms of the views held by students. After a review of broad trends in enrollment in Catholic higher education globally and regionally over the last four decades, and a discussion of some of the increasing competitive pressures Catholic (and other) universities are confronted with, this article explores some of the factors that may lead students to enroll in Catholic higher education institutions. Values and faith matter, but (not surprisingly) other factors matter more. The paper also discusses the possibility for Catholic universities to invest in service learning as an opportunity to strengthen their Catholic identity in a way that respects pluralism in the student body.