bluuebunny/crossref_metadata_2025 huggingface dataset:
Regularizing Effects for a Singular Elliptic Problem
Authors: de Bonis Ida , Porzio Maria Michaela
Published: January 2025

In this paper, we prove existence and regularity results for a nonlinear elliptic problem of p-Laplacian type with a singular potential like fuγ and a lower order term bu, where u is the solution and b and f are only assumed to be summable functions. We show that, despite the lack of regularity of the data, for suitable choices of the function b in the lower order term, a strong regularizing effect appears. In particular we exhibit the existence of bounded solutions. Worth notice is that this result fails if b≡0, i.e., in absence of the lower order term. Moreover, we show that, if the singularity is “not too large” (i.e., γ≤1), such a regular solution is also unique.
Adaptive Transfer Learning Strategy for Predicting Battery Aging in Electric Vehicles
Authors: Galatro Daniela , Shroff Manav , Amon Cristina H.
Published: January 2025

This work presents an adaptive transfer learning approach for predicting the aging of lithium-ion batteries (LiBs) in electric vehicles using capacity fade as the metric for the battery state of health. The proposed approach includes a similarity-based and adaptive strategy in which selected data from an original dataset are transferred to a clean dataset based on the combined/weighted similarity contribution of feature and stress factor similarities and times series similarities. Transfer learning (TL) is then performed by pre-training a model with clean data, with frozen weights and biases to the hidden layer. At the same time, weights and biases toward the output node are recalculated with the target data. The error reduction lies between −0.4% and −8.3% for 20 computational experiments, attesting to the effectiveness and robustness of our adaptive TL approach. Considerations for data structure and representation learning are presented, as well as a workflow to enhance the application of transfer learning for predicting aging in LiBs.
High Glucose Concentration on the Metabolic Activity of C6 Glia Cells: Implication in Alzheimer’s Disease
Authors: Hernández-Contreras Karla Aketzalli , Rojas-Durán Fausto , Hernández-Aguilar María Elena , Herrera-Covarrubias Deissy , Godinez-Victoria Marycarmen , Manzo-Denes Jorge , Pérez-Estudillo César Antonio , Ramos-Morales Fernando Rafael , Toledo-Cárdenas Rebeca , Aranda-Abreu Gonzalo Emiliano
Published: January 2025

Background: Alzheimer’s disease (AD), the leading cause of dementia worldwide, poses an increasing global health burden, yet its pathogenesis remains poorly understood. Diabetes mellitus (DM), characterized by chronic hyperglycemia, has been identified as a significant risk factor for AD development, suggesting a potential metabolic and molecular link between these diseases. Methods: This study examines the impact of sustained high glucose levels on astrocyte-like C6 glial cells, focusing on key cellular processes associated with AD. We evaluated mitochondrial function, oxidative stress, glucose uptake, and the expression of hallmark AD proteins, including β-amyloid and hyperphosphorylated tau. Results: Our findings demonstrate that high glucose exposure triggers mitochondrial hyperactivity, oxidative stress, and increased Tau phosphorylation, though β-amyloid levels were unaffected within the experimental timeframe. Conclusions: These results shed light on the early cellular dysfunctions contributing to the DM-AD connection, providing valuable insights into the metabolic pathways involved and identifying potential therapeutic targets to mitigate AD progression in individuals with DM.
Role of Vitamin D Status and Alterations in Gut Microbiota Metabolism in Fibromyalgia-Associated Chronic Inflammatory Pain
Authors: Saija Caterina , Bertuccio Maria Paola , Scoglio Alberto , Macaione Vincenzo , Cacciola Francesco , Micalizzi Giuseppe , Caccamo Daniela , Muscoli Carolina , Currò Monica
Published: January 2025

Background/Objectives: Several studies suggest gut microbiota metabolites as important immuno-modulators in inflammatory pain. We aimed to investigate the relationship between vitamin D status and gut dysbiosis markers in fibromyalgia (FM)-associated chronic inflammation. Methods: Blood samples were collected from sixty-eight female FM patients (49.9 ± 12.35 years). Pain intensity was assessed by FIQ-R. The serum levels of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, IL-17, IFN-γ, as well as those of vitamin D (25(OH)D3) and the kynurenine/tryptophan ratio (Kyn/Trp) were determined by ELISA and HPLC, respectively. The plasma levels of the SCFAs acetate, butyrate, and propionate were detected by GC-MS. Results: A mean FIQ-R score indicated that the patients could be classified as having moderate FM. The mean levels of all cytokines, but IL-6 and IL-1β, were higher than the normal reference values. The highest concentrations of cytokines were observed in patients showing the highest FIQ-R scores and the lowest 25(OH)D3 levels. Deficient levels of acetate were found paralleled by an increase in Kyn/Trp. The highest acetate concentrations were detected in patients with the lowest FIQ-R scores and 25(OH)D3 levels. Significantly negative correlations were found between 25(OH)D3 concentrations and FIQ-R scores (p = 0.007) as well as IL-17 levels (p = 0.002) and between acetate and TNF-α (p = 0.040) as well as FIQ-R scores (p = 0.028), while significantly positive correlations were observed between Kyn/Trp and IL-17 (p = 0.027) as well as IFN-γ (p = 0.003). Conclusions: Our preliminary data suggest that the vitamin D status along with altered gut microbiota metabolism plays a major role in FM-related inflammatory pain. Replication of these findings in a larger cohort is required to provide additional insights.
Multisensory Stimulation in Rehabilitation of Dementia: A Systematic Review
Authors: Calderone Andrea , Marra Angela , De Luca Rosaria , Latella Desirèe , Corallo Francesco , Quartarone Angelo , Tomaiuolo Francesco , Calabrò Rocco Salvatore
Published: January 2025

Background/Objectives: Dementia leads to cognitive decline, affecting memory, reasoning, and daily activities, often requiring full-time care. Multisensory stimulation (MSS), combined with cognitive tasks, can slow this decline, improving mood, communication, and overall quality of life. This systematic review aims to explore methods that utilize MSS in the rehabilitation of patients with dementia. Its clinical value is rooted in its ability to offer a deep comprehension of how MSS can be successfully incorporated into rehabilitation treatments. Methods: Studies were identified from an online search of PubMed, EBSCOhost, Cochrane Library, Web of Science, Embase, and Scopus databases with a search time frame from 2014 to 2024. This review has been registered on Open OSF (n) 3KUQX. Results: Pilot studies investigating MSS interventions, encompassing Cognitive Stimulation Therapy (CST), Sonas therapy, and combined physical–cognitive exercise programs, have yielded mixed findings in individuals with dementia. CST has demonstrated significant improvements in general cognitive function, particularly in language skills, offering a promising approach for cognitive enhancement. Sonas therapy, while showing positive trends in some studies, does not consistently achieve statistically significant outcomes across all cognitive domains. Conversely, combined exercise programs have shown efficacy in improving dual-task performance, suggesting benefits for motor–cognitive integration. MSS delivered within specialized environments like Snoezelen rooms consistently produces positive effects on mood, reducing agitation and promoting relaxation. Conclusions: This review emphasizes how MSS can enhance cognitive, emotional, and behavioral results for individuals with dementia. It is essential for future research to standardize protocols, incorporate advanced technologies such as virtual reality, and rectify diversity gaps. Collaboration between different fields will improve the effectiveness and usefulness of MSS in caring for individuals with dementia.
Evidence of Inflammatory Network Disruption in Chronic Venous Disease: An Analysis of Circulating Cytokines and Chemokines
Authors: Fraile-Martinez Oscar , García-Montero Cielo , Gomez-Lahoz Ana María , Sainz Felipe , Bujan Julia , Barrena-Blázquez Silvestra , López-González Laura , Díaz-Pedrero Raul , Álvarez-Mon Melchor , García-Honduvilla Natalio , Saez Miguel A. , Monserrat Jorge , Ortega Miguel A.
Published: January 2025

Background: Chronic venous disease (CVD) comprises a set of vascular disorders that affect the venous system with important local and systemic repercussions. A growing body of evidence displays the relationship between suffering from CVD and a marked deregulation of the immune inflammatory system. In this sense, the previous literature has reported some significant changes in the level of various circulating inflammatory parameters in these patients. However, more research is required to detail and deepen this complex relationship. Methods: In this work, we studied, using a multiplex technique, the levels of circulating cytokines and chemokines detectable in the serum of 40 patients with CVD and compared it with 38 healthy controls (HCs). In parallel, we performed Spearman’s correlation analysis to explore potential inflammatory networks in CVD. Results: In this study, we measured circulating cytokines and chemokines in CVD patients using a multiplex assay. Results showed increased levels of several pro-inflammatory mediators (IL-1β, IL-2, IL-5, IL-6, IL-7, IL-8, IL-12, IL-17A, IL-23, TNF-α, IFN-γ, fractalkine, ITAC, and GM-CSF) and a decrease in IL-13, with no significant changes in IL-4, IL-10, IL-21, MIP-1α, MIP-1β, or MIP-3α. The Spearman correlation analysis revealed strong, positive correlations among several inflammatory mediators in HC, particularly between TNF-alpha, IL-1β, IL-17A, and IL-23, forming a highly interconnected cytokine network. In contrast, CVD patients showed fewer, weaker, and distinct correlations, with new associations such as IFN-γ with IL-1β and IL-23, suggesting a disrupted inflammatory profile. Conclusions: The distinct inflammatory profile in CVD patients, characterized by altered cytokine and chemokine levels and a less coordinated cytokine network, underscores the reconfiguration of inflammatory pathways in this condition. These findings highlight potential therapeutic targets aimed at restoring immune balance and mitigating chronic inflammation in CVD.
Validation of an Upgraded Virtual Reality Platform Designed for Real-Time Dialogical Psychotherapies
Authors: Simoes-Gomes Taylor , Potvin Stéphane , Giguère Sabrina , Beaudoin Mélissa , Phraxayavong Kingsada , Dumais Alexandre
Published: January 2025

Background: The advent of virtual reality in psychiatry presents a wealth of opportunities for a variety of psychopathologies. Avatar Interventions are dialogic and experiential treatments integrating personalized medicine with virtual reality (VR), which have shown promising results by enhancing the emotional regulation of their participants. Notably, Avatar Therapy for the treatment of auditory hallucinations (i.e., voices) allows patients to engage in dialogue with an avatar representing their most persecutory voice. In addition, Avatar Intervention for cannabis use disorder involves an avatar representing a significant person in the patient’s consumption. In both cases, the main goal is to modify the problematic relationship and allow patients to regain control over their symptoms. While results are promising, its potential to be applied to other psychopathologies, such as major depression, is an exciting area for further exploration. In an era where VR interventions are gaining popularity, the present study aims to investigate whether technological advancements could overcome current limitations, such as avatar realism, and foster a deeper immersion into virtual environments, thereby enhancing participants’ sense of presence within the virtual world. A newly developed virtual reality platform was compared to the current platform used by our research team in past and ongoing studies. Methods: This study involved 43 subjects: 20 healthy subjects and 23 subjects diagnosed with severe mental disorders. Each participant interacted with an avatar using both platforms. After each immersive session, questionnaires were administered by a graduate student in a double-blind manner to evaluate technological advancements and user experiences. Results: The findings indicate that the new technological improvements allow the new platform to significantly surpass the current platform as per multiple subjective parameters. Notably, the new platform was associated with superior realism of the avatar (d = 0.574; p < 0.001) and the voice (d = 1.035; p < 0.001), as well as enhanced lip synchronization (d = 0.693; p < 0.001). Participants reported a significantly heightened sense of presence (d = 0.520; p = 0.002) and an overall better immersive experience (d = 0.756; p < 0.001) with the new VR platform. These observations were true in both healthy subjects and participants with severe mental disorders. Conclusions: The technological improvements generated a heightened sense of presence among participants, thus improving their immersive experience. These two parameters could be associated with the effectiveness of VR interventions and future studies should be undertaken to evaluate their impact on outcomes.
Surface Plasmon Resonance-Based Biodetection Systems: Principles, Progress and Applications—A Comprehensive Review
Authors: Butt Muhammad A.
Published: January 2025

Surface Plasmon Resonance (SPR)-based biodetection systems have emerged as powerful tools for real-time, label-free biomolecular interaction analysis, revolutionizing fields such as diagnostics, drug discovery, and environmental monitoring. This review highlights the foundational principles of SPR, focusing on the interplay of evanescent waves and surface plasmons that underpin its high sensitivity and specificity. Recent advancements in SPR technology, including enhancements in sensor chip materials, integration with nanostructures, and coupling with complementary detection techniques, are discussed to showcase their role in improving analytical performance. The paper also explores diverse applications of SPR biodetection systems, ranging from pathogen detection and cancer biomarker identification to food safety monitoring and environmental toxin analysis. By providing a comprehensive overview of technological progress and emerging trends, this review underscores the transformative potential of SPR-based biodetection systems in addressing critical scientific and societal challenges. Future directions and challenges, including miniaturization, cost reduction, and expanding multiplexing capabilities, are also presented to guide ongoing research and development in this rapidly evolving field.
Diagnostic Errors in the Acutely Dizzy Patient—Lessons Learned
Authors: Tarnutzer Alexander A. , Koohi Nehzat , Lee Sun-Uk , Kaski Diego
Published: January 2025

Acute vertigo or dizziness is a frequent presentation to the emergency department (ED), making up between 2.1% and 4.4% of all consultations. Given the nature of the ED where the priority is triage, diagnostic delays and misdiagnoses are common, with as many as a third of vertebrobasilar strokes presenting with acute vertigo or dizziness being missed. Here, we review diagnostic errors identified in the evaluation and treatment of the acutely dizzy patient and discuss strategies to overcome them. Lessons learned include focusing on structured history taking, asking about timing and triggers to inform a targeted examination, assessing subtle ocular motor findings (e.g., by use of HINTS(+)), and avoiding overreliance on brain imaging (including early magnetic resonance imaging including diffusion-weighted sequences [DWI-MRI]). Importantly, up to 20% of DWI-MRI may be false negatives if obtained within the first 24–48 h after symptom onset. Likewise, overreliance on focal neurologic findings to confirm a stroke diagnosis should be avoided because isolated dizziness, vertigo, or even unsteadiness may be the only symptoms in some patients with vertebrobasilar stroke. Furthermore, in patients with triggered episodic vestibular symptoms provocation maneuvers should be preferred over HINTS(+), and a potential diagnosis of stroke should not be immediately dismissed in younger patients presenting with a headache (where migraine may be more common), but the possibility of a vertebral artery dissection should be further evaluated. Importantly, moderate training of non-experts allows for significant improvement in diagnostic accuracy in the acutely dizzy patient and thus should be prioritized.
Dose-Escalated SBRT for Borderline and Locally Advanced Pancreatic Cancer: Resectability Rate and Pathological Results of a Multicenter Prospective Study
Authors: Salas-Salas Barbara , Ferrera-Alayon Laura , Espinosa-Lopez Alberto , Perez-Rodriguez Maria Luisa , Afonso Antonio Alayón , Vera-Rosas Andres , Garcia-Plaza Gabriel , Chicas-Sett Rodolfo , Martinez-Martin Maria Soledad , Salcedo Elisa , Kannemann Andrea , Lloret-Saez-Bravo Marta , Lara Pedro C.
Published: January 2025

Objective: We demonstrated for the first time the safety and feasibility of escalating up to 55 Gy/11 Gy/fr/5fr in borderline (BRPC)/unresectable locally advanced pancreatic cancer (LAPC), using the standard LINAC platform. The aim of the present study is to assess for the first time the impact of this high-dose neoadjuvant stereotactic ablative radiotherapy (SABRT) protocol on tumor resectability and pathological responses. Materials/Methods: From June 2017 to December 2022, patients with BRPC/LAPC were treated with neoadjuvant chemotherapy (ChT) and SABRT-escalated doses of SIB at 45 Gy, 50 Gy, and up to 55 Gy (BED ≥ 100). Radiological evaluation was conducted with a CT scan 6-8 weeks post-treatment to determine resectability status based on established criteria (SAR/APA2014). Surgical decisions were made by the multidisciplinary tumor board of the participating institutions. Pathological assessments post-surgery used criteria from the College of American Pathologists (CAP), categorizing resection status as R0 (negative margins), R1 (microscopic tumor margins), and R2 (macroscopic tumor margins). Tumor response was evaluated with the Tumor Response Scoring (TRS) system, as G0 (no viable cancer cells), G1 (single cells or rare small groups), G2 (residual cancer with evident regression), and G3 (extensive residual cancer). Results: Thirty-three patients (p) were included: 39.4% (13p) BRPC/60.6% (20p) LAPC. After ChT-SABRT, 45.5% (15p) were considered resectable, with 11/13 (84.6%) BRPC and 4/20 (20%) LAPC (p < 0.0001). One patient refused surgery and other patient died of COVID sepsis. Two more patients had disseminated disease at surgery. Among the 11 patients who underwent full surgery, all patients achieved either clean margins R0: 72.7% (8p) or microscopic affected margins R1: 27.3% (3p). TRS scores were G1: 27.3% (3p), G2: 54.5% (6p), and G3: 18.2% (2p). The present follow-up (FUP) was closed on 1 November 2024 (23.55 months, range: 6–71 months). The mean freedom from local progression as the first cause of disease failure was 43.30 ± 3.09 (37.23–49.38), and the median was not reached. The actuarial 1- and 2-year rates for freedom from local relapse as a first cause of disease failure were 92.3% (87.7–93.3%) and 79.7% (79.7–87.7%), respectively. Conclusions: Neoadjuvant ChT-SABRT in LAPC improves resectability rates and induces relevant tumor regression. These promising findings should be validated by larger sample sizes and extended follow-up.
Ruthenium(II) Complex with 8-Hydroxyquinoline Exhibits Antitumor Activity in Breast Cancer Cell Lines
Authors: Khalifa Amr , Sheweita Salah , Namatalla Asmaa , Khalifa Mohamed , Nencioni Alessio , Sultan Ahmed
Published: January 2025

Background/Objectives: Breast cancer (BC) remains one of the most prevalent and deadly cancers worldwide, with limited access to advanced treatments in developing regions. There is a critical need for novel therapies with unique mechanisms of action, especially to overcome resistance to conventional platinum-based drugs. This study investigates the anticancer potential of the ruthenium complex Bis(quinolin-8-olato)bis(triphenylphosphine)ruthenium(II) (Ru(quin)2) in ER-positive (T47D) and triple-negative (MDA-MB-231) BC cell lines. Results: Ru(quin)2 demonstrated dose-dependent cytotoxicity, with IC50 values of 48.3 μM in T47D cells and 45.5 μM in MDA-MB-231 cells. Its cytotoxic effects are primarily driven by apoptosis, as shown by increased BAX expression, enhanced caspase-3 activity, reduced Aurora B kinase levels, and elevated histone release. Ru(quin)2 also induced autophagy, evidenced by LC3-I to LC3-II conversion and reduced SQSTM1, partially mediated through MAPK signaling. Furthermore, Ru(quin)2 induced G0/G1 cell cycle arrest by downregulating cyclin D1, CDK4, and CDK6, alongside upregulation of the CDK inhibitor p21. Conclusions: Ru(quin)2 emerges as a potent candidate for BC treatment, with multiple mechanisms of action involving apoptosis, autophagy, and cell cycle arrest. Further studies are warranted to elucidate its detailed molecular mechanisms and evaluate its therapeutic potential in vivo, moving toward clinical applications for both ER-positive and triple-negative BC management.
The Bi-Modified (BiO)2CO3/TiO2 Heterojunction Enhances the Photocatalytic Degradation of Antibiotics
Authors: Gao Yue , Cao Tieping , Du Jinfeng , Qi Xuan , Yan Hao , Xu Xuefeng
Published: January 2025

The increasing concentration of antibiotics in natural water poses a significant threat to society’s sustainable development due to water pollution. Photocatalytic technology is an efficient and environmentally friendly approach to environmental purification, offering great potential for addressing pollution and attracting significant attention from scholars worldwide. TiO2, as a representative semiconductor photocatalytic material, exhibits strong oxidation ability and excellent biocompatibility. However, its wide band gap and the rapid recombination of photo-generated electron–hole pairs significantly limit its photocatalytic applications. Recent studies indicate that constructing heterojunctions with synergistic plasmonic effects is an effective strategy for developing high-performance photocatalysts. In this study, Bi metal nanoparticles and (BiO)2CO3 nanosheets were simultaneously grown on TiO2 nanofibers via an in situ hydrothermal method, successfully forming a Bi@(BiO)2CO3/TiO2 composite fiber photocatalyst with synergistic plasmonic effects. The surface plasmon resonance (SPR) effect of Bi nanoparticles combined with the (BiO)2CO3/TiO2 heterojunction enhances sunlight absorption, facilitates efficient separation of photo-generated carriers, and significantly strengthens the photo-oxidation and reduction abilities. This system effectively generates abundant hydroxyl (·OH) and superoxide (·O2−) radicals under sunlight excitation. Consequently, Bi@(BiO)2CO3/TiO2 exhibited outstanding photocatalytic performance. Under simulated sunlight for 60 min, the photodegradation efficiencies of the quinolone antibiotics lomefloxacin, ciprofloxacin, and norfloxacin reached 93.2%, 97.5%, and 100%, respectively. Bi@(BiO)2CO3/TiO2 also demonstrates excellent stability and reusability. This study represents a significant step toward the application of TiO2-based photocatalyst materials in environmental purification.
Anti-Inflammatory Effects of Cannabigerol In Vitro and In Vivo Are Mediated Through the JAK/STAT/NFκB Signaling Pathway
Authors: Jeong Ga Hee , Kim Ki Chan , Lee Ji Hyun
Published: January 2025

Cannabinoid compounds have potential as treatments for a variety of conditions, with cannabigerol (CBG) being known for its anti-inflammatory properties. In this study, we investigated the effects of CBG in a cellular model of 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD). In the cellular model, we confirmed the cytotoxicity of CBG and downregulated the expression of inflammatory markers CCL26, IL1B, IL6, and TNF (p < 0.001). In the mouse model, clinical, histological, and immunological changes were analyzed. The results showed that CBG improved dermatitis severity score, epidermal thickness, and mast cell count and reduced inflammatory cytokines (Tslp, Il1b, Il4, Il6, Il13, Il17, Il18, Il22, and Il33) by qRT-PCR (p < 0.001). Western blot results showed modulated changes in JAK1, JAK2, TYK2, STAT1, STAT2, STAT3, p-STAT3, STAT6, and p-STAT6 (p < 0.05). Subsequently, p-IκBα, NF-κB, and p-NF-κB signaling factors were also reduced (p < 0.05), with corresponding changes in skin barrier factors. The results of this study indicate that CBG effectively alleviates AD-like symptoms and suggest the potential of CBG as a therapeutic agent.
Dietary Beliefs and Their Association with Overweight and Obesity in the Spanish Child Population
Authors: Murillo-Llorente María Teresa , Palau-Ferrè Alma María , Legidos-García María Ester , Pérez-Murillo Javier , Tomás-Aguirre Francisco , Lafuente-Sarabia Blanca , Asins-Cubells Adalberto , Martínez-Peris Miriam , Ventura Ignacio , Casaña-Mohedo Jorge , Pérez-Bermejo Marcelino
Published: January 2025

Background/Objectives: Childhood obesity is a multifactorial chronic disease that represents one of the main preventable causes of morbidity and mortality. This study analyzes how nutritional beliefs influence eating habits and the prevalence of overweight and obesity in Spanish children and adolescents. Methods: A cross-sectional study was conducted in 35 educational centers in 12 Spanish provinces, with a sample of 1131 children and adolescents aged 6 to 14 years. Anthropometric and sociodemographic data were collected, and dietary habits were assessed by means of questionnaires. Statistical analyses were used to identify associations between dietary beliefs and body mass index. Results: In total, 29.5% of participants were overweight or obese. Two groups of beliefs were identified: healthier beliefs and less healthy beliefs. Children with less healthy dietary beliefs had a significantly higher BMI (22.16 kg/m2) compared to those with healthier beliefs (17.2 kg/m2). False nutritional beliefs, influenced by advertising and the family environment, contribute to overweight and obesity. Discussion: Dietary beliefs play a crucial role in determining eating habits and, therefore, the health of children. Nutrition education and public policies that promote healthy eating habits are essential to prevent childhood obesity. It is important to involve the family, the school, and the media in these efforts. Conclusions: Despite efforts, many children continue to hold erroneous nutritional beliefs that contribute to the rise in overweight and obesity. This study highlights the importance of addressing dietary beliefs and promoting appropriate nutrition education to prevent childhood obesity. It is recommended to implement educational strategies and public policies that regulate the advertising of unhealthy foods and promote healthy eating habits.
Agroclimatic Zoning of Temperature Limitations for Growth of Stubble Cover Crops
Authors: Haberle Jan , Chuchma Filip , Raimanova Ivana , Wollnerova Jana
Published: January 2025

The realization of the expected benefits of stubble cover crops (CCs) depends on sufficient plant growth, which is influenced by the sum of effective temperatures (SET) before the onset of winter and the occurrence of the first early autumn frost (FRST). The objective of this study was to calculate the SET for three dates of CC sowing, August 20 (A), September 6 (B), and September 20 (C), from 1961 to 2020, based on daily data from 268 meteorological stations in the Czech Republic (CR). The dates of FRST, when the daily average and minimum temperatures at 2 m and the minimum temperature at the ground level fell below 0 °C, −3, and −5 °C during CC growth, were recorded. The analysis showed a significant trend in the average SET, which increased by 1.60, 0.87, and 0.97 °C per year for scenarios A, B, and C, respectively. As a result, the area where SET conditions allowed for CC flowering from autumn sowing expanded, as visualized in the agroclimatic maps of the country. The average dates of the FRST shifted by 0.05–0.11 days per year over the sixty years, but this was not significant due to high inter-annual variability. The SET was closely related to the average annual temperature and station elevation (r = ǀ0.95ǀ–ǀ0.99ǀ), while the corresponding trend relationships were weaker (r = ǀ0.40ǀ–ǀ0.43ǀ). This study provides data on the zonation of the conditions required to achieve specific CC management objectives.
Study on Preparation and Flame-Retardant Mechanism of Cerium-Doped Mg-Al Hydrotalcite
Authors: Li Yanan , Shen Genli , Liu Mi , Wang Zhen , Gong Yan , Ma Yong , Ji Daiyu , Li Jianqiang , Yang Min , Wang Qi
Published: January 2025

Cerium-doped hexagonal lamellar hydrotalcite was prepared via the hydrothermal method and applied to epoxy resin (EP) composites. The characterization results of cerium-doped hydrotalcite showed that the interlayer spacing of hydrotalcite increased with an increase in the cerium doping amount, and an appropriate amount of cerium doping will not destroy the layered structure of hydrotalcite. This study also determined the best cerium doping ratio. The combustion performance of the composite was studied using thermogravimetric infrared analysis, smoke density test, and limiting oxygen index (LOI). The results showed that the addition of cerium-doped hydrotalcite reduced the amount of smoke and the escape of hydrocarbons during the combustion and decomposition of epoxy resin. The main flame-retardant mechanism was that the addition of cerium-doped Mg-Al hydrotalcite promoted the formation of a dense protective layer on the surface of the composite to inhibit the escape of combustible substances, so as to achieve the purpose of a flame retardant and smoke suppression.
Micellization and Physicochemical Properties of CTAB in Aqueous Solution: Interfacial Properties, Energetics, and Aggregation Number at 290 to 323 K
Authors: Naskar Bappaditya
Published: January 2025

This work reports a detailed investigation of the micellization of the cationic surfactant cetyltrimethylammonium bromide (CTAB) over the temperature range of 290–313 K. Conductometry, tensiometry, fluorimetry, and isothermal titration calorimetry (ITC) were used to study the overall solution behavior of amphiphilic self-aggregation. The CMC values showed a trend of first declining and then rising with a minimum temperature of 298 K. The adsorption at the air–water interface and micellization processes of CTAB are both spontaneous. The enthalpy of CTAB micellization is negative at 290 K and increases negatively as the temperature rises. The interfacial parameters—maximum surface excess concentration (Γmax), minimum area per molecule (Amin), standard free energy of adsorption (Amin), and surface pressure at CMC (ΠCMC)—were calculated using surface tension data. The aggregation numbers (N) of CTAB micelles and others (SDS and CHAPS) determined at different [surfactant]>CMC by the static fluorescence quenching method were used to find out the N values at CMC (or NCMC). The results revealed that the NCMC values were 48, 54, and 4 for CTAB, SDS, and CHAPS micelles, respectively. Temperature-dependent NCMC by the ITC method was also examined for the studied surfactants. Additionally, the ITC-determined specific heat of micellization was used to find out the extent of water penetration into the micelle interior of up to 8, 7, and 3 hydrocarbons for CTAB, SDS, and CHAPS, respectively.
Optimization and Prediction of the Mechanical Properties of Concrete with Crumb Rubber and Stainless-Steel Fibers Under Varying Temperatures
Authors: El-Zohairy Ayman , Hamdy Osman
Published: January 2025

This research develops an equation to describe the relationship between stress (σ) and strain (ε) in concrete under different conditions. It includes important parameters from earlier studies to improve predictions of stress–strain behavior, especially for concrete with crumb rubber and stainless-steel fibers at various temperatures. The initial phase assessed three existing stress–strain formulas as a basis for optimization. Using the Genetic Algorithm (GA) and the Whale Optimization Algorithm (WOA), a new equation was created to simulate the stress–strain relationship while considering temperature changes and material additions. Results showed that Formula (1), optimized with the WOA, performed much better than other polynomial and exponential formulas, proving the WOA’s effectiveness over the traditional GA. A comparison of the mechanical properties from experiments and those predicted by the new formula showed a high level of accuracy. Key properties like the maximum stress, strain at maximum stress, modulus of elasticity, and toughness were well captured. The findings highlight how temperature and material composition significantly affect concrete’s mechanical behavior. Overall, this research offers important insights into the factors influencing concrete performance, providing a solid framework for future studies and practical applications in engineering and construction. The proposed formula is a reliable tool for predicting concrete’s mechanical properties under various conditions, which aids in better modeling and optimization in concrete design.
Impact of Mutations in Soybean Oleate and Linoleate Desaturase Genes on Seed Germinability of Heat-Stressed Plants
Authors: Toyinbo Johnson O. , Saripalli Gautam , Ingole Hrishikesh P. , Jones Zachary T. , Naveed Salman , Noh Enoch , Narayanan Sruthi , Rustgi Sachin
Published: January 2025

Soybean is the primary oilseed crop in the United States, with significant industrial value. Understanding the molecular mechanisms of heat stress tolerance in soybean plants is critical for developing stress-resistant cultivars. Current knowledge about the role of fatty acid desaturases (FADs) in modulating membrane fluidity under abiotic stress prompted this investigation into the impact of mutations in the FAD genes on seed germination from heat-stressed plants. In soybean plants, exposure to heat stress during anthesis is known to significantly reduce seed germination. In silico expression analysis indicated high expression levels of the soybean FAD2 and FAD3 genes in the leaves. Therefore, a detailed expression analysis of these genes was conducted using qRT-PCR from leaf tissue. Generally, downregulation of these genes was observed in the mutants; however, two genes, FAD3A and FAD2-3, showed a more than 2-fold increase in expression in six out of ten mutants under heat stress. This upregulation was particularly pronounced (7-fold) in the mutant S17CR-170. Correlation analysis revealed a positive correlation (up to 0.48) between the expression level of FAD3A, FAD3B, FAD3C, and FAD2-3 and the decline in germination from heat-stressed plants. This suggests these FAD genes may act as negative regulators of germination under heat stress conditions.
Impact of Ag Coating Thickness on the Electrochemical Behavior of Super Duplex Stainless Steel SAF2507 for Enhanced Li-Ion Battery Cases
Authors: Jo Hyeongho , Ok Jung-Woo , Lee Yoon-Seok , Lee Sanghun , Je Yonghun , Kim Shinho , Kim Seongjun , Park Jinyong , Hong Jonggi , Lee Taekyu , Shin Byung-Hyun , Yoon Jang-Hee , Kim Yangdo
Published: January 2025

Li-ion batteries are at risk of explosions caused by fires, primarily because of the high energy density of Li ions, which raises the temperature. Battery cases are typically made of plastic, aluminum, or SAF30400. Although plastic and aluminum aid weight reduction, their strength and melting points are low. SAF30400 offers excellent strength and corrosion resistance but suffers from work hardening and low high-temperature strength at 700 °C. Additionally, Ni used for plating has a low current density of 25% international copper alloy standard (ICAS). SAF2507 is suitable for use as a Li-ion battery case material because of its excellent strength and corrosion resistance. However, the heterogeneous microstructure of SAF2507 after casting and processing decreases the corrosion resistance, so it requires solution heat treatment. To address these issues, in this study, SAF2507 (780 MPa, 30%) is solution heat-treated at 1100 °C after casting and coated with Ag (ICAS 108.4%) using physical vapor deposition (PVD). Ag is applied at five different thicknesses: 0.5, 1.0, 1.5, 2.0, and 2.5 μm. The surface conditions and electrochemical properties are then examined for each coating thickness. The results indicate that the PVD-coated surface forms a uniform Ag layer, with electrical conductivity increasing from 1.9% ICAS to 72.3% ICAS depending on the Ag coating thickness. This enhancement in conductivity can improve Li-ion battery safety on charge and use. This result is expected to aid the development of advanced Li-ion battery systems in the future.
Morphogenesis and Organogenesis to Hatching of the Green Octopus, Octopus hubbsorum: A Species That Includes a Paralarval Stage in Its Life Cycle
Authors: García-Flores Maritza , Arellano-Martínez Marcial , Aguilar-Cruz Carlos A. , Rodríguez-Jaramillo M. Carmen
Published: January 2025

This work describes the morphological changes during embryonic development and pre-hatching organ development of Octopus hubbsorum, a species with a paralarval phase in its life cycle. The morphogenesis of O. hubbsorum begins simultaneously with the first reversion, a typical characteristic of octopuses, along with the development of the embryonic shape and structure. However, this species hatches with only three well-developed suckers. Organogenesis starts after the first embryonic reversion, with the development of the eyes, optic lobes, digestive gland, arms, and mantle. The branchial and central hearts show optimal development before the second reversion. The ink sac develops in the late stages (after the second reversion), while the stomach and crop are evident only after hatching and continue to develop until the first feeding. Newly hatched organisms show a complete development of the nervous, respiratory, circulatory, and integumentary systems. During the paralarval phase, O. hubbsorum undergoes major morphological and physiological changes until it reaches the subadult phase and develops the reproductive organs. This study highlights important organogenic differences compared to species with planktonic and benthic hatchlings. Further studies are needed in species with different life strategies to expand our knowledge of the developmental biology of cephalopods.
Effects of Different Voided Urine Sample Storage Time, Temperature, and Preservatives on Analysis with Multiplex Bead-Based Oncuria Bladder Cancer Immunoassay
Authors: Tanaka Sunao , Murakami Kaoru , Sakatani Toru , Lee Riko , Hogrefe Wayne , Siguencia Fernando , Rosser Charles J. , Furuya Hideki
Published: January 2025

Background/Objectives: Urinalysis accuracy requires reliable sample stability that is dependent on the chosen collection and storage conditions. The multiplex Oncuria bladder cancer immunoassay currently needs urine samples stored at 4 °C until analysis, which requires more effort, equipment, and workflow than storing samples at room temperature. Thus, successful sample storage at room temperature (20 °C) may reduce laboratory handling time and expenses. This study evaluated whether different voided urine sample collection and storage parameters affected subsequent biomarker analysis with Oncuria. The Oncuria simultaneously quantifies 10 protein analytes in urine to generate a bladder cancer diagnostic signature. Methods: Samples were stored at varied temperatures (20 °C, 4 °C, −20 °C) for up to 1 month. The effects of adding two commercial urine sample stabilizers and antibiotics (trimethoprim) were also assessed. Subsequently, multiple potential biospecimen stabilizers were tested in urine samples and evaluated with Oncuria in hopes of allowing the urine sample to remain at room temperature for extended periods of time. Results: First, it was demonstrated that voided urine samples stored at room temperate without such stabilizers had different levels of the 10 analytes associated with the Oncuria test compared to voided urine samples stored at 4 °C. Next, we evaluated the effects of commercially available biospecimen stabilizers. Despite the addition of these stabilizers, the levels of the 10 analytes were altered when the samples were stored at room temperature for prolonged periods of time. Therefore, we could not identify a suitable biospecimen stabilizer that would not require sample refrigeration. Conclusions: To minimize sample degradation/alteration after collection, voided urine samples should be refrigerated until analyzed with Oncuria as the refrigeration is advantageous for the storage and the transport of these urine samples.
Prevalence and Molecular Characterization of Carbapenemase-Producing Multidrug-Resistant Bacteria in Diabetic Foot Ulcer Infections
Authors: Saleem Mohd , Moursi Soha Abdallah , Altamimi Tahani Nasser Almofeed , Alharbi Mohammed Salem , Alaskar Alwaleed Mohammad , Hammam Sahar Adly Hassan , Rakha Ehab , Syed Muhammad Ozair Ilyas , Almalaq Hamoud Abdulmohsin , Alshammari Metab Nasser , Syed Khaja Azharuddin Sajid
Published: January 2025

Background: Diabetic foot ulcers (DFUs) represent severe complications in diabetic patients, often leading to chronic infections and potentially resulting in nontraumatic lower-limb amputations. The increasing incidence of multidrug-resistant (MDR) bacteria in DFUs complicates treatment strategies and worsens patient prognosis. Among these pathogens, carbapenemase-producing pathogens have emerged as particularly concerning owing to their resistance to β-lactam antibiotics, including carbapenems. Methods: This study evaluated the prevalence of MDR bacteria, specifically carbapenemase-producing pathogens, in DFU infections. A total of 200 clinical isolates from DFU patients were analyzed via phenotypic assays, including the modified Hodge test (MHT) and the Carba NP test, alongside molecular techniques to detect carbapenemase-encoding genes (blaKPC, blaNDM, blaVIM, blaIMP, and blaOXA-48). Results: Among the isolates, 51.7% were confirmed to be carbapenemase producers. The key identified pathogens included Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli. The most commonly detected carbapenemase genes were blaKPC (27.6%) and blaNDM (24.1%). Carbapenemase-producing isolates presented high resistance to β-lactam antibiotics, whereas non-carbapenemase-producing isolates presented resistance through mechanisms such as porin loss and efflux pumps. Conclusions: The findings of this study highlight the significant burden of MDR infections, particularly carbapenemase-producing organisms, in DFUs. MDR infections were strongly associated with critical clinical parameters, including pyrexia (p = 0.017), recent antibiotic use (p = 0.003), and the severity of infections. Notably, the need for minor amputations was much higher in MDR cases (p < 0.001), as was the need for major amputations (p < 0.001). MDR infections were also strongly associated with polymicrobial infections (p < 0.001). Furthermore, Wagner ulcer grade ≥II was more common in MDR cases (p = 0.002). These results emphasize the urgent need for enhanced microbiological surveillance and the development of tailored antimicrobial strategies to combat MDR pathogens effectively. Given the high prevalence of carbapenem resistance, there is an immediate need to explore novel therapeutic options to improve clinical outcomes for diabetic patients with DFUs.
Minimally Invasive Surgical Approach in Granulomatosis with Polyangiitis Complicated with Intramural Descending Aorta Hematoma Followed by Aortic Wall Rupture
Authors: Ciobica Mihai-Lucian , Botezatu Alexandru-Sebastian , Galajda Zoltan , Carsote Mara , Nistor Claudiu , Sandulescu Bianca-Andreea
Published: January 2025

Background and Clinical Significance: Granulomatosis with polyangiitis (GPA) represents a rare autoimmune disease with granulomatous inflammation, tissue necrosis, and systemic vasculitis of the small and medium blood vessels. Although the clinical elements vary, aortic involvement is exceptional and it represents a challenge that requires a rapid intervention with the potential of displaying a fulminant evolution. Case Presentation: We report a 64-year-old male with an 18-year history of GPA who presented atypical low back pain. Following ultrasound and computed tomography exams, the initial suspicion was an intramural descending aorta hematoma, surrounded by a peri-aortic sleeve suggesting a chronic inflammation. Serial non-invasive assessments revealed a progressive lesion within the next 10 to 12 days to an aortic wall rupture, despite the absence of previous aneurysmal changes. The peri-aortic fibrous inflammatory sleeve was life-saving, and emergency minimally invasive surgery was successful, including the massive improvement in back pain. Conclusions: To our knowledge, this is a very rare scenario in GPA; we found only 18 other cases (the oldest report being from 1994). An interventional approach was mentioned in a few cases as seen in this instance. Glucocorticoid medication for GPA might act as a potential contributor to symptomatic osteoporotic fractures which require a prompt differential diagnosis. Unusual aortic manifestations (such as intramural aortic hematoma or aortic wall rupture) are difficult to recognize since the index of clinical suspicion is rather low. A prompt intervention may be life-saving and a multidisciplinary team is mandatory. Minimally invasive surgical correction of the aortic event represents an optimum management in the modern era. Such cases add to the limited data we have so far with respect to unusual outcomes in long-standing GPAs.
Shoulder Physiological Offset Parameters in Asian Populations—A Magnetic Resonance Imaging Study
Authors: Huang Hung-Yi , Lin Meng-Hao , Hsu Chu-Hsiang , Kuo Liang-Tseng
Published: January 2025

Background/Objectives: Physical shoulder offset parameters (SOPs) play important roles in the diagnosis and treatment of shoulder diseases. However, there is little research analyzing SOPs in healthy shoulders using cross-sectional MRI images, especially in Asians. Therefore, this study aimed to establish physiological reference values of shoulder parameters for Asian populations. Methods: This was a retrospective imaging study using MRI images of the shoulder joints of 500 patients (mean age: 55.9 ± 14.0 years). We measured the following SOPs of the normal joint: HO, GO, lateral glenoidal humeral offset (LGHO), humeral shaft axis offset (HAO), and cortical offset (CO). In addition, the offset parameters were examined for associations with age, gender, side, and osteoarthritis. Results: The mean HO was 22.9 (±2.4) mm, the mean GO was 62.3 (±6.6) mm, the mean LGHO was 48.9 (±4.2) mm, the mean HAO was 25.2 (±2.8) mm, and the mean CO was 15.7 (±2.7) mm. Male patients exhibited significantly higher values across all SOPs compared to female patients. In addition, there was a significantly lower mean value for HAO in left shoulders (HAO: 24.7± 2.8 mm vs. 25.5 ± 2.8 mm, p = 0.011). There was a negatively significant correlation between age and all SOPs. No significant difference in mean values was noted between shoulders with osteoarthritis and non-osteoarthritis in any SOPs. Conclusions: Significant gender- and age-specific differences were noted for all measured SOPs. In addition, right shoulders did not show higher mean SOP values than left shoulders, except for HAO, suggesting that the contralateral joint is a reliable reference for surgical planning. These findings should be considered in shoulder surgery planning.