bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevalence of Common Mental Disorders in South Asia: A Systematic Review and Meta-Regression Analysis
Authors: Naveed Sadiq , Waqas Ahmed , Chaudhary Amna Mohyud Din , Kumar Sham , Abbas Noureen , Amin Rizwan , Jamil Nida , Saleem Sidra
Published: September 2020

South Asian countries report the highest prevalence of common mental disorders (CMDs) globally. This systematic review and meta-analysis report the pooled prevalence of CMDs among the South Asian countries. Database searches were conducted in eight electronic databases. Titles, abstracts, full-text screening, and extraction of data on the event rate of 17 indicators of CMDs were performed by two independent reviewers. A total of 160 studies were included and data analysis was done using the Comprehensive Meta-analysis Software (v.3). A prevalence of depressive symptoms was 26.4% among 173,449 participants, alcohol abuse was 12.9% (n = 107,893); anxiety 25.8% (n = 70,058); tobacco smoking 18.6% (n = 84,965); PTSD 17.2% (n = 42,298); mixed anxiety and depression 28.4% (n = 11,102); suicidal behaviors 6.4% (n = 25,043); misuse of opiates 0.8% (n = 37,304); tobacco chewing 21.0% (n = 10,586); use of cannabis 3.4% (n = 10,977); GAD 2.9% (n = 70,058); bipolar disorder 0.6% (n = 7,197); IV drug abuse 2.5% (n = 15,049); panic disorder 0.01% (n = 28,087); stimulant use 0.9% (n = 1,414); OCD 1.6% (n = 8,784) and phobic disorders 1.8% (n = 27,754). This study reported a high prevalence of CMDs in South Asian countries; necessitating further research on psychiatric epidemiology in those contexts. It informs the need for effective policymaking and implementation of culturally appropriate multilevel interventions.
Luteal Presence and Ovarian Response at the Beginning of a Timed Artificial Insemination Protocol for Lactating Dairy Cows Affect Fertility: A Meta-Analysis
Authors: Borchardt Stefan , Pohl Alina , Heuwieser Wolfgang
Published: September 2020

Progesterone (P4) concentration during follicular growth has a major impact on fertility response in timed artificial insemination (TAI) protocols. Luteal presence at the beginning of a TAI protocol and ovarian response after the first gonadotropin-releasing hormone (GnRH) injection (G1) affect P4 concentration and subsequently pregnancy per artificial insemination (P/AI). A systematic review of the literature and meta-analytical assessment was performed with the objective of evaluating the magnitude of the effect of luteal presence and ovarian response at the beginning of a TAI protocol on P/AI in lactating dairy cows. We considered only studies using synchronisation protocols consisting of GnRH and prostaglandin F 2α. The time interval between G1 and prostaglandin F 2α (PGF 2α) had to range from 5 to 7 d. The time interval between the PGF 2α injection and G2 had to range from 48 to 72 h. We used 28 controlled experiments from 27 published manuscripts including 16,489 cows with the objective of evaluating the effect size of having a functional corpus luteum (CL) at G1 on P/AI. Information regarding ovulatory response after G1 was available for 5676 cows. In a subset of cows (n = 4291), information was available for luteal presence and ovulatory response at the initiation of the TAI protocol. A functional CL at G1 increased (p < 0.001) the relative risk of conceiving (RR (relative risk) = 1.32; 95% CI = 1.21–1.45) in lactating dairy cows. Ovulation after G1 increased (p < 0.001) the relative risk of conceiving (RR = 1.29; 95% CI = 1.20–1.38) in lactating dairy cows. The effect of ovulatory response on P/AI after G1 was affected by luteal presence at G1. In summary, there was a clear benefit on P/AI for cows starting a TAI protocol with a functional CL (+10.5 percentage units) and cows ovulating at the beginning of a TAI protocol (+11.0 percentage units).
Examining the Effects of Whole Crop Wheat Silage on Ewe Performance during Late Gestation Compared to Traditional Grass Silage across Three Prolific Breed Types
Authors: Higgins Jonathan T. , Campion Dermot , Jones Joe , Lott Stephen , Lynch M. Bridget , McEvoy Mary , McGovern Fiona , Boland Tommy M.
Published: September 2020

Provision of adequate nutrient intake in late gestation of the ewe is an important determinant of dam and offspring performance. A 2 × 3 factorial design experiment examining two forage types, whole crop wheat silage (WCWS) or grass silage (GS) offered to one of three prolific breed types, (Belclare X, Lleyn X, Mule (Bluefaced Leicester × Blackface Mountain)), was conducted. Forage type had no impact on dry matter (DM) or metabolizable energy (ME) intake, body weight and body condition score change, or colostrum production (p > 0.05). Ewes offered WCWS had lower crude protein (CP) intake (p < 0.0001) and a lower combined litter weight (p < 0.05). Mule ewes consumed less DM, CP, (p < 0.05), and ME (p < 0.01) compared to Belclare X and Lleyn X ewes however, water intake per kg DM consumed did not differ with breed type (p > 0.05). Colostrum yield over the first 18 h postpartum was lower for Mule ewes compared to other breed types (p < 0.05). In conclusion, results from this study suggest nutrient concentration and balance as opposed to forage type is important for late gestation nutrition and breed type can impact feed intake and colostrum yield.
Seasonal Expression of NGF and Its Cognate Receptors in the Ovaries of Grey Squirrels (Sciurus carolinensis)
Authors: Maranesi Margherita , Palermo Francesco Alessandro , Bufalari Antonello , Mercati Francesca , Paoloni Daniele , Cocci Paolo , Moretti Giulia , Crotti Silvia , Zerani Massimo , Dall’Aglio Cecilia
Published: September 2020

The grey squirrel is an invasive alien species that seriously threatens the conservation of the native red squirrel species. With the aim of characterizing the reproductive physiology of this species due to its great reproductive success, the function of the ovarian nerve growth factor (NGF) system was analyzed in a grey squirrel population living in central Italy. During the breeding and nonbreeding seasons, the ovarian presence, distribution, and gene expression of NGF, neurotrophic tyrosine kinase receptor 1 (NTRK1), and nerve growth factor receptor (NGFR), as well as NGF plasma concentrations, were evaluated in female grey squirrels. NGF was found in the luteal cells and in the thecal and granulosa cells of follicles, while NTRK1 and NGFR were only observed in follicular thecal and granulosa cells. NGF and NGFR transcripts were almost two-fold greater during the breeding season, while no seasonal differences were observed in NTRK1 gene expression. During the breeding season, NGFR was more expressed than NTRK1. Moreover, no changes were observed in NGF plasma levels during the reproductive cycle. The NGF system seems to be involved in regulating the ovarian cycle mainly via local modulation of NGF/NGFR, thus playing a role in the reproductive physiology of this grey squirrel population.
Interval between Removal of a 4.7 mg Deslorelin Implant after a 3-, 6-, and 9-Month Treatment and Restoration of Testicular Function in Tomcats
Authors: Ferré-Dolcet Lluis , Carniello Lisa , Ferro Silvia , Cattai Andrea , Romagnoli Stefano , Mollo Antonio
Published: September 2020

Deslorelin implants have been used to produce a reversible sterilization in several species. In cats, the prolonged duration (12–15 months in tomcats and 18–22 months in queen) is often too much for cat breeders who request early implant removal. The interval between implant removal and resumption of reproductive function in cats has never been investigated. Eighteen tomcats received a 4.7 mg deslorelin implant placed in the periumbilical area and surgically removed during all seasons of the year after 3, 6, or 9 months (n = 6, 6, and 6 cats, respectively). Following implant removal, all cats received a clinical exam every two weeks, including testicular ultrasonographic measurement, observation of penile spikes, and blood collection for serum testosterone assay. Restoration of serum testosterone secretion occurred after 23 ± 6, 23 ± 6, and 22 ± 7 days in the 3-, 6-, and 9-month groups, respectively. Restoration of testicular function was confirmed by histology in 13/15 cats undergoing orchiectomy at the end of the study while the owners of the remaining two cats opted to maintain their animals intact. Removal of a 4.7 mg deslorelin implant after 3, 6, or 9 months is followed by resumption of serum testosterone secretion after about 3 weeks independent of age or season.
Optimizing the Use of iPSC-CMs for Cardiac Regeneration in Animal Models
Authors: Bizy Alexandra , Klos Matthew
Published: September 2020

Heart failure (HF) is a common disease in which the heart cannot meet the metabolic demands of the body. It mostly occurs in individuals 65 years or older. Cardiac transplantation is the best option for patients with advanced HF. High numbers of patient-specific cardiac myocytes (CMs) can be generated from induced pluripotent stem cells (iPSCs) and can possibly be used to treat HF. While some studies found iPSC-CMS can couple efficiently to the damaged heart and restore cardiac contractility, almost all found iPSC-CM transplantation is arrhythmogenic, thus hampering the use of iPSC-CMs for cardiac regeneration. Studies show that iPSC-CM cultures are highly heterogeneous containing atrial-, ventricular- and nodal-like CMs. Furthermore, they have an immature phenotype, resembling more fetal than adult CMs. There is an urgent need to overcome these issues. To this end, a novel and interesting avenue to increase CM maturation consists of modulating their metabolism. Combined with careful engineering and animal models of HF, iPSC-CMs can be assessed for their potential for cardiac regeneration and a cure for HF.
Evaluation of Rainfall Forecasts by Three Mesoscale Models during the Mei-yu Season of 2008 in Taiwan. Part II: Development of an Object-Oriented Method
Authors: Wang Chung-Chieh , Paul Sahana , Lee Dong-In
Published: September 2020

This study describes a recently developed object-oriented method suitable for Taiwan for the purpose to verify quantitative precipitation forecasts (QPFs) produced by mesoscale models as a complement to the traditional approaches in existence. Using blended data from the rain-gauge network in Taiwan and the Tropical Rainfall Measuring Mission (TRMM) as the observation, the method developed herein is applied to twice-daily 0–48 h QPFs produced by the Cloud-Resolving Storm Simulator (CReSS) during the South-West Monsoon Experiment (SoWMEX) in May–June 2008. In this method, rainfall objects are identified through a procedure that includes smoothing and thresholding. Various attribute parameters and the characteristics of observed and forecast rain-area objects are then compared and discussed. Both the observed and the QPF frequency distributions of rain-area objects with respect to total water production, object size, and rainfall are similar to chi-distribution, with highest frequency at smaller values and decreased frequencies toward greater values. The model tends to produce heavier rainfall than observation, while the latter exhibits a higher percentage of larger objects with weaker rainfall intensity. The distributions of shape-related attributes are similar between QPF and observed rainfall objects, with more northeast–southwest oriented and fewer northwest–southeast oriented objects. Both observed and modeled object centroid locations have relative maxima over the terrain of Taiwan, indicating reasonable response to the topography. The above results are consistent with previous studies.
Reducing a Class of Two-Dimensional Integrals to One-Dimension with an Application to Gaussian Transforms
Authors: Straton Jack C.
Published: September 2020

Quantum theory is awash in multidimensional integrals that contain exponentials in the integration variables, their inverses, and inverse polynomials of those variables. The present paper introduces a means to reduce pairs of such integrals to one dimension when the integrand contains powers multiplied by an arbitrary function of xy/(x+y) multiplying various combinations of exponentials. In some cases these exponentials arise directly from transition-amplitudes involving products of plane waves, hydrogenic wave functions, and Yukawa and/or Coulomb potentials. In other cases these exponentials arise from Gaussian transforms of such functions.
Investigating Gait, Movement, and Coordination in Children with Neurodevelopmental Disorders: Is There a Role for Motor Abnormalities in Atypical Neurodevelopment?
Authors: Colizzi Marco , Ciceri Marco , Di Gennaro Gianfranco , Morari Beatrice , Inglese Alessandra , Gandolfi Marialuisa , Smania Nicola , Zoccante Leonardo
Published: September 2020

Motor abnormalities have been suggested to play a role in most neuropsychiatric disorders, as a potential generic neurodevelopmental vulnerability. However, they still represent a neglected area, with a paucity of empirical studies, especially in pediatric populations. This case-control study aimed to comprehensively assess motor functioning in children with atypical neurodevelopment and investigate whether any socio-demographic or clinical characteristics would concur with motor difficulties to distinguish children with neurodevelopmental disorders (NDD) from healthy controls. Socio-demographic (age and gender) and clinical (intelligence quotient, gait, movement, and coordination) data were collected on 114 children aged 5–15 (83 with NDD, 31 healthy controls). Male children were at significantly higher risk for NDD (OR: 13.023, p < 0.001). Furthermore, there was a statistically significant interaction between the total intelligence quotient and overall coordination such that increasing levels of total intelligence quotient appeared to protect against the likelihood of being diagnosed with an NDD, but only in the context of a preserved coordination (OR: 0.964, p = 0.038). Collectively, results may have important public health implications, as they point towards the development of new approaches to establish an early prognosis in neurodevelopment, including assessing motor difficulties and mitigating their impact on children’s quality of life.
Heat Shock Protein 70 (HSP70) Induction: Chaperonotherapy for Neuroprotection after Brain Injury
Authors: Kim Jong Youl , Barua Sumit , Huang Mei Ying , Park Joohyun , Yenari Midori A. , Lee Jong Eun
Published: September 2020

The 70 kDa heat shock protein (HSP70) is a stress-inducible protein that has been shown to protect the brain from various nervous system injuries. It allows cells to withstand potentially lethal insults through its chaperone functions. Its chaperone properties can assist in protein folding and prevent protein aggregation following several of these insults. Although its neuroprotective properties have been largely attributed to its chaperone functions, HSP70 may interact directly with proteins involved in cell death and inflammatory pathways following injury. Through the use of mutant animal models, gene transfer, or heat stress, a number of studies have now reported positive outcomes of HSP70 induction. However, these approaches are not practical for clinical translation. Thus, pharmaceutical compounds that can induce HSP70, mostly by inhibiting HSP90, have been investigated as potential therapies to mitigate neurological disease and lead to neuroprotection. This review summarizes the neuroprotective mechanisms of HSP70 and discusses potential ways in which this endogenous therapeutic molecule could be practically induced by pharmacological means to ultimately improve neurological outcomes in acute neurological disease.
Intracellular Trafficking of HBV Particles
Authors: Jiang Bingfu , Hildt Eberhard
Published: September 2020

The human hepatitis B virus (HBV), that is causative for more than 240 million cases of chronic liver inflammation (hepatitis), is an enveloped virus with a partially double-stranded DNA genome. After virion uptake by receptor-mediated endocytosis, the viral nucleocapsid is transported towards the nuclear pore complex. In the nuclear basket, the nucleocapsid disassembles. The viral genome that is covalently linked to the viral polymerase, which harbors a bipartite NLS, is imported into the nucleus. Here, the partially double-stranded DNA genome is converted in a minichromosome-like structure, the covalently closed circular DNA (cccDNA). The DNA virus HBV replicates via a pregenomic RNA (pgRNA)-intermediate that is reverse transcribed into DNA. HBV-infected cells release apart from the infectious viral parrticle two forms of non-infectious subviral particles (spheres and filaments), which are assembled by the surface proteins but lack any capsid and nucleic acid. In addition, naked capsids are released by HBV replicating cells. Infectious viral particles and filaments are released via multivesicular bodies; spheres are secreted by the classic constitutive secretory pathway. The release of naked capsids is still not fully understood, autophagosomal processes are discussed. This review describes intracellular trafficking pathways involved in virus entry, morphogenesis and release of (sub)viral particles.
2D Photogrammetry as a Forwarder Load Measurement Technique
Authors: Nuhlíček Ondřej , Slavík Martin , Dvořák Jiří
Published: September 2020

Research Highlights: In this study, we present the use of time-lapse photos as a way to estimate the height of the load on the forwarders. This approach, using nonprofessional outdoor cameras, is a cheap and time-effective solution for continuous load height measurements, and it offers at least the same accuracy as a gauge measurement. This method represents another approach to the automation of time studies in forestry. Background and Objectives: Time studies require information about the load on the forwarders. Until now, this information was obtained either by using a gauge measure, sampling of the load, or averaging the load from large area datasets. More accurate methods like laser scanning are costly and fragile. During time study preparations, we suggested a robust system of measuring the load height and tested it against the commonly used gauge measuring technique. Materials and Methods: Two cameras took pictures of the load; these photos were processed for camera lens distortion and rectified into the cartesian coordinate system, and the height of the load was calculated. These values were then tested against gauge measured values using paired t-test. Results: Straight line distance calculated from the images and the gauge-measured distance did not show a significant difference (p-value 0.9354). Calculated vertical distance was, however, significantly different from the calculated straight-line distance (p-value of 0.0015), suggesting possible bias of the gauge measured distance. The root mean square error (RMSE) of the rectification process was, on average, 0.42 cm. Conclusions: The proposed method was verified to correspond with the gauge measure method; however, our research raised the question of the gauge method reliability, as the taken measurements are not perfectly vertical, and for the correct load estimation, the vertical distance is needed. We, therefore, conclude that for this photogrammetry method, the vertical, rather than straight-line, distance should be used. The presented solution can be used for long-term data collection without interrupting the whole forwarding process for taking the load measurement. The longer data processing in office enables researchers to spend less time in the field taking hand measurements.
How Urban Forest Managers Evaluate Management and Governance Challenges in Their Decision-Making
Authors: Ordóñez Camilo , Kendal Dave , Threlfall Caragh G. , Hochuli Dieter F. , Davern Melanie , Fuller Richard A. , van der Ree Rodney , Livesley Stephen J.
Published: September 2020

Decisions about urban forests are critical to urban liveability and resilience. This study aimed to evaluate the range of positions held by urban forest managers from local governments in the state of Victoria, Australia, regarding the management and governance challenges that affect their decision-making. This study was based on a Q-method approach, a procedure that allows researchers to evaluate the range of positions that exist about a topic in a structured manner based on the experiences of a wide group of people. We created statements on a wide range of urban forest management and governance challenges and asked urban forest managers to rate their level of agreement with these statements via an online survey. Managers generally agreed about the challenges posed by urban development and climate change for implementing local government policies on urban forest protection and expansion. However, there were divergent views about how effective solutions based on increasing operational capacities, such as increasing budgets and personnel, could address these challenges. For some managers, it was more effective to improve critical governance challenges, such as inter-departmental and inter-municipal coordination, community engagement, and addressing the culture of risk aversion in local governments. Urban forest regional strategies aimed at coordinating management and governance issues across cities should build on existing consensus on development and environmental threats and address critical management and governance issues not solely related to local government operational capacity.
Impact of Simulated Gastrointestinal Digestion on the Biological Activity of an Alcalase Hydrolysate of Orange Seed (Siavaraze, Citrus sinensis) by-Products
Authors: Mazloomi Seyadeh Narges , Mora Leticia , Aristoy M-Concepción , Mahoonak Alireza Sadeghi , Ghorbani Mohammad , Houshmand Gholamreza , Toldrá Fidel
Published: September 2020

In this study, orange seed proteins were hydrolyzed by Alcalase enzyme at different enzyme concentrations 1–3% (v/w) and hydrolysis times (2–5 h), to obtain bioactive peptides showing antioxidant, Angiotensin-converting enzyme (ACE) -inhibitory, and hypoglycemic activities. The highest biological activities (p < 0.05) were achieved by using a hydrolysis time of 5 h and an enzyme concentration of 2%. Orange seed protein hydrolysate (OSPH) was prepared under these conditions, and peptides were isolated and purified by using size-exclusion chromatography and high-performance liquid chromatography, respectively. The fractions that showed the highest biological activities were analyzed by mass spectrometry in tandem, and a total of 63 peptide sequences were found. Moreover, the effect of simulated gastrointestinal digestion on the bioactivity of the fractions was studied, and the novel peptide sequences generated were also identified. Overall, despite there being some differences in the profile of peptide sequences obtained, the main results showed non-significant differences in the analyzed bioactivities after simulated gastrointestinal digestion.
Identification of a Novel Variant in EARS2 Associated with a Severe Clinical Phenotype Expands the Clinical Spectrum of LTBL
Authors: Barbosa-Gouveia Sofia , González-Vioque Emiliano , Hermida Álvaro , Suarez María Unceta , Martínez-González María Jesús , Borges Filipa , Wintjes Liesbeth , Kappen Antonia , Rodenburg Richard , Couce María-Luz
Published: September 2020

The EARS2 nuclear gene encodes mitochondrial glutamyl-tRNA synthetase, a member of the class I family of aminoacyl-tRNA synthetases (aaRSs) that plays a crucial role in mitochondrial protein biosynthesis by catalyzing the charging of glutamate to mitochondrial tRNA(Glu). Pathogenic EARS2 variants have been associated with a rare mitochondrial disorder known as leukoencephalopathy with thalamus and brainstem involvement and high lactate (LTBL). The targeted sequencing of 150 nuclear genes encoding respiratory chain complex subunits and proteins implicated in the oxidative phosphorylation (OXPHOS) function was performed. The oxygen consumption rate (OCR), and the extracellular acidification rate (ECAR), were measured. The enzymatic activities of Complexes I-V were analyzed spectrophotometrically. We describe a patient carrying two heterozygous EARS2 variants, c.376C>T (p.Gln126*) and c.670G>A (p.Gly224Ser), with infantile-onset disease and a severe clinical presentation. We demonstrate a clear defect in mitochondrial function in the patient’s fibroblasts, suggesting the molecular mechanism underlying the pathogenicity of these EARS2 variants. Experimental validation using patient-derived fibroblasts allowed an accurate characterization of the disease-causing variants, and by comparing our patient’s clinical presentation with that of previously reported cases, new clinical and radiological features of LTBL were identified, expanding the clinical spectrum of this disease.
Identification of Pathways Associated with Placental Adaptation to Maternal Nutrient Restriction in Sheep
Authors: Edwards Ashley K. , Dunlap Kathrin A. , Spencer Thomas E. , Satterfield M. Carey
Published: September 2020

Maternal nutrient restriction impairs placental growth and development, but available evidence suggests that adaptive mechanisms exist, in a subset of nutrient restricted (NR) ewes, that support normal fetal growth and do not result in intrauterine growth restriction (IUGR). This study utilized Affymetrix GeneChip Bovine and Ovine Genome 1.0 ST Arrays to identify novel placental genes associated with differential fetal growth rates within NR ewes. Singleton pregnancies were generated by embryo transfer and, beginning on Day 35 of pregnancy, ewes received either a 100% National Research Council (NRC) (control-fed group; n = 7) or 50% NRC (NR group; n = 24) diet until necropsy on Day 125. Fetuses from NR ewes were separated into NR non-IUGR (n = 6) and NR IUGR (n = 6) groups based on Day 125 fetal weight for microarray analysis. Of the 103 differentially expressed genes identified, 15 were upregulated and 88 were downregulated in NR non-IUGR compared to IUGR placentomes. Bioinformatics analysis revealed that upregulated gene clusters in NR non-IUGR placentomes associated with cell membranes, receptors, and signaling. Downregulated gene clusters associated with immune response, nutrient transport, and metabolism. Results illustrate that placentomal gene expression in late gestation is indicative of an altered placental immune response, which is associated with enhanced fetal growth, in a subpopulation of NR ewes.
Genome-Wide Identification and Characterization of Lectin Receptor-Like Kinase Gene Family in Cucumber and Expression Profiling Analysis under Different Treatments
Authors: Lv Duo , Wang Gang , Xiong Liang-Rong , Sun Jing-Xian , Chen Yue , Guo Chun-Li , Yu Yao , He Huan-Le , Cai Run , Pan Jun-Song
Published: September 2020

Lectin receptor-like kinases (LecRLKs) are a class of membrane proteins found in plants that are involved in diverse functions, including plant development and stress responses. Although LecRLK families have been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in cucumber (Cucumis sativus L.). In this study, 46 putative LecRLK genes were identified in the cucumber genome, including 23 G-type and 22 L-type, and one C-type LecRLK gene. They were unequally distributed on all seven chromosomes, with a clustering tendency. Most of the genes in the cucumber LecRLK (CsLecRLK) gene family lacked introns. In addition, there were many regulatory elements associated with phytohormones and stress on these genes’ promoters. Transcriptome data demonstrated distinct expression patterns of CsLecRLK genes in various tissues. Furthermore, we found that each member of the CsLecRLK family had its own unique expression pattern under hormone and stress treatment by the quantitative real-time PCR (qRT-PCR) analysis. This study provides a better understanding of the character and function of the LecRLK gene family in cucumber and opens up the possibility to exploring the roles that LecRLKs might play in the life cycle of cucumber.
Whole Mitochondrial Genome Analysis in Serbian Cases of Leber’s Hereditary Optic Neuropathy
Authors: Dawod Phepy G. A. , Jancic Jasna , Marjanovic Ana , Brankovic Marija , Jankovic Milena , Samardzic Janko , Potkonjak Dario , Djuric Vesna , Mesaros Sarlota , Novakovic Ivana , Abdel Motaleb Fayda I. , Kostic Vladimir S. , Nikolic Dejan
Published: September 2020

Leber’s hereditary optic neuropathy (LHON) is a maternally inherited disorder that affects central vision in young adults and is typically associated with mitochondrial DNA (mtDNA) mutations. This study is based on a mutational screening of entire mtDNA in eight Serbian probands clinically and genetically diagnosed with LHON and four of their family members, who are asymptomatic mutation carriers. All obtained sequence variants were compared to human mtDNA databases, and their potential pathogenic characteristics were assessed by bioinformatics tools. Mitochondrial haplogroup analysis was performed by MITOMASTER. Our study revealed two well-known primary LHON mutations, m.11778G>A and m.3460G>A, and one rare LHON mutation, m.8836A>G. Various secondary mutations were detected in association with the primary mutations. MITOMASTER analysis showed that the two well-known primary mutations belong to the R haplogroup, while the rare LHON m.8836A>G was detected within the N1b haplogroup. Our results support the need for further studies of genetic background and its role in the penetrance and severity of LHON.
Mechanical Crop Load Management (CLM) Improves Fruit Quality and Reduces Fruit Drop and Alternate Bearing in European Plum (Prunus domestica L.)
Authors: Lammerich Sebastian , Kunz Achim , Damerow Lutz , Blanke Michael
Published: September 2020

(1) Background: With ca. 10 million tons of annual production worldwide, the plum (Prunus ssp.) ranks as a major fruit crop and can suffer from small fruit size, premature fruit drop and alternate bearing, which are addressed in this paper using a range of crop load management (CLM) tools. (2) Methods: Sixty 10-year-old European plum cv. “Ortenauer” trees on dwarfing St. Julien INRA GF 655/2 rootstock (slender spindle; 4.25 × 2.80 m) in a commercial orchard near Bonn (50°N), Germany, were thinned in 2 years and flower intensity assessed in the following year. Thinning was performed either mechanically (type Bonn/Baum) or chemically, with ATS (ammonium thiosulfate) or ethephon (Flordimex), or by a combination of mechanical and chemical methods, to improve fruit quality and the proportion of Class 1 fruit. Adjacent un-thinned trees served as controls. (3) Results: Natural fruit drop in June was reduced from 290 fruits per tree in the un-thinned controls to 265 fruits after ATS blossom treatment, and to 148 fruits after mechanical thinning at 380 rpm at a 5 km/h tractor speed at full bloom. The un-thinned control trees developed a large number of small, undersized fruits. The yield of Class 1 fruits increased per tree from 47% in the un-thinned controls, up to 69% after crop load management. Sugar content and fruit firmness were unaffected. (4) Conclusions: The study has shown that fruit quality (i.e., fruit size) and financial returns could be improved by either mechanical (380 rpm at 5 km/h) or chemical thinning, or a combination of both.
Patient-Perceived Benefit of Treatment in Polypoidal Choroidal Vasculopathy: A Pilot Study
Authors: Ghoshal Rituparna , Sharanjeet-Kaur Sharanjeet , Fadzil Norliza Mohamad , Ghosh Somnath , Ngah Nor Fariza , Aziz Roslin Azni Abd
Published: September 2020

Polypoidal choroidal vasculopathy (PCV), a subtype of neovascular age-related macular degeneration, requires repeated treatment. The objective of this pilot study was to evaluate and compare vision-targeted quality of life (QOL) at baseline and after 6 months of treatment in patients with PCV. Naive PCV patients were recruited. Visual functions assessed were distance visual acuity (DVA), near visual acuity (NVA), contrast sensitivity (CS), reading speed (RS), and QOL at baseline and after 6 months of treatment. Thirty patients (average age of 67.62 ± 8.05 years) revealed mean DVA and NVA improvements of 0.24 logMAR and 0.30 logMAR, respectively. Mean CS and RS improved by 0.39 log contrast and 25.58 words per minute, respectively. The National Eye Institute Visual Function Questionnaire 25 (NEI-VFQ-25) composite score significantly increased from a baseline of 66.73 ± 13.74 to 73.54 ± 14.26. Twenty-eight of the patients showed overall improvement in QOL score by 5 units or more or remained stable. Subscales of NEI-VFQ-25 significantly improved, with general vision, mental health, and role difficulties improving by 10 or more units. The present pilot study reports a significant improvement of QOL in PCV patients after 6 months of treatment, with mental health, role difficulties, social functioning, and distance vision activities being the most improved subscales.
Does Believing in Fate Facilitate Active or Avoidant Coping? The Effects of Fate Control on Coping Strategies and Mental Well-Being
Authors: Wu Wesley C. H. , Chen Sylvia Xiaohua , Ng Jacky C. K.
Published: September 2020

The development of control-related constructs has involved different approaches over time, and yet internal and external locus of control are conceptualized as dichotomous factors influencing active versus avoidant coping strategies. While external control is associated with avoidance, a similar belief construct fate control, which denotes that life events are pre-determined and influenced by external forces but predictable and alterable, challenges the assumption of incompatibility between fate and agency. To develop a dynamic model of control, we suggest that external control would affect avoidant coping, which in turn would affect psychological distress, whereas fate control would affect both active and avoidant coping when dealing with stress. The model was supported among Hong Kong Chinese using a cross-sectional approach in Study 1 (n = 251) and hypothetical stressful scenarios in Study 2 (n = 294). The moderating effect of perceived controllability was observed in coping behaviors using a diary approach in Study 3 (n = 188). Our findings offer an alternative perspective to the dichotomous view of control and provide implications for coping strategies and mental well-being.
Knowledge, Attitude, and Practices of Healthcare Professionals on COVID-19 and Risk Assessment to Prevent the Epidemic Spread: A Multicenter Cross-Sectional Study from Punjab, Pakistan
Authors: Malik Usman Rashid , Atif Naveel , Hashmi Furqan Khurshid , Saleem Fahad , Saeed Hamid , Islam Muhammad , Jiang Minghuan , Zhao Mingyue , Yang Caijun , Fang Yu
Published: September 2020

In the current outbreak of novel coronavirus (COVID-19), healthcare professionals (HCPs) have a primary role in combating the epidemic threat. HCPs are at high risk of not only contracting the infection but also spreading it unknowingly. It is of utmost importance to evaluate their knowledge, attitudes, and practices (KAP) and the ability to assess the risks associated with the outbreak. A cross-sectional online survey involving physicians, pharmacists, and nurses was conducted. A 39-itemed questionnaire based on the World Health Organization (WHO)COVID-19 risk assessment tool was shared with healthcare professionals in three purposively selected key divisions of Punjab province. Out of 500 healthcare professionals, 385 responded to the survey. The majority (70%) were aged 22–29 years; 144 (37.4%) physicians, 113 (29.4%) nurses, and 128 (33.2%) pharmacists completed the survey. Overall, 94.8% of healthcare professionals scored adequately (>14) for COVID-19-related knowledge; 97.9% displayed an optimistic attitude (>42) and 94.5% had an adequate practice score (>28). Kruskal–Wallis and Jonckheere–Terpstra tests showed significant differences (p < 0.05) in KAP and risk assessment scores among groups; physicians and nurses scored higher as compared to pharmacists. Further research and follow-up investigations on disaster management and risk assessment can help policy-makers better tackle future epidemics.
Decoding Susceptibility to Respiratory Viral Infections and Asthma Inception in Children
Authors: Read James F. , Bosco Anthony
Published: September 2020

Human Respiratory Syncytial Virus and Human Rhinovirus are the most frequent cause of respiratory tract infections in infants and children and are major triggers of acute viral bronchiolitis, wheezing and asthma exacerbations. Here, we will discuss the application of the powerful tools of systems biology to decode the molecular mechanisms that determine risk for infection and subsequent asthma. An important conceptual advance is the understanding that the innate immune system is governed by a Bow-tie architecture, where diverse input signals converge onto a few core pathways (e.g., IRF7), which in turn generate diverse outputs that orchestrate effector and regulatory functions. Molecular profiling studies in children with severe exacerbations of asthma/wheeze have identified two major immunological phenotypes. The IRF7hi phenotype is characterised by robust upregulation of antiviral response networks, and the IRF7lo phenotype is characterised by upregulation of markers of TGFβ signalling and type 2 inflammation. Similar phenotypes have been identified in infants and children with severe viral bronchiolitis. Notably, genome-wide association studies supported by experimental validation have identified key pathways that increase susceptibility to HRV infection (ORMDL3 and CHDR3) and modulate TGFβ signalling (GSDMB, TGFBR1, and SMAD3). Moreover, functional deficiencies in the activation of type I and III interferon responses are already evident at birth in children at risk of developing febrile lower respiratory tract infections and persistent asthma/wheeze, suggesting that the trajectory to asthma begins at birth or in utero. Finally, exposure to microbes and their products reprograms innate immunity and provides protection from the development of allergies and asthma in children, and therefore microbial products are logical candidates for the primary prevention of asthma.
Failures of the Cast-Iron Columns of Historic Buildings—Case Studies
Authors: Gołdyn Michał , Urban Tadeusz
Published: September 2020

Selected technical problems related to the rehabilitation of cast-iron columns in structures from the turn of the 19th and 20th century are discussed in the paper. Lack of contemporary standard regulations related to the design of cast-iron structures is a significant problem in the design works and experimental investigations on cast-iron columns are frequently required. The paper presents results of the tests concerning principal properties of cast-iron—strength and deformability. The historical design principles are discussed in the light of the results of experimental investigations. As it was demonstrated, the actual load-carrying capacities of cast-iron columns may exceed by several times the values resulting from the 20th century design rules. The conservatism of the design principles resulted, however, from the material uncertainties—lack of homogeneity and hidden defects of the cast-iron. Selected examples of failures of cast-iron columns from 19th-century structures such as post-industrial buildings and engineering structures are discussed. They resulted from errors made during adaptation works. The reasons for these failures and considered methods of repairing the structures are presented.
Rational Behavior in Dynamic Multicriteria Games
Authors: Rettieva Anna
Published: September 2020

We consider a dynamic, discrete-time, game model where n players use a common resource and have different criteria to optimize. To construct a multicriteria Nash equilibrium the bargaining solution is adopted. To design a multicriteria cooperative equilibrium, a modified bargaining scheme that guarantees the fulfillment of rationality conditions is applied. The concept of dynamic stability is adopted for dynamic multicriteria games. To stabilize the multicriteria cooperative solution a time-consistent payoff distribution procedure is constructed. The conditions for rational behavior, namely irrational-behavior-proofness condition and each step rational behavior condition are defined for dynamic multicriteria games. To illustrate the presented approaches, a dynamic bi-criteria bioresource management problem with many players is investigated.
Antimicrobial Resistance Gene Detection and Plasmid Typing Among Multidrug Resistant Enterococci Isolated from Freshwater Environment
Authors: Cho Sohyun , Barrett John B. , Frye Jonathan G. , Jackson Charlene R.
Published: September 2020

In this study, mechanisms of antimicrobial resistance (AR) as well as the abundance and diversity of plasmids were determined among multidrug resistant (MDR) enterococci from surface water in GA, USA. A total of 51 enterococci isolates were screened for the presence of 27 AR genes conferring resistance to ciprofloxacin, erythromycin, tylosin, kanamycin, streptomycin, lincomycin, Quinupristin/Dalfopristin (Q/D), and tetracycline. A plasmid classification system based on replication genes was used to detect 19 defined Gram-positive plasmid replicon families. Twelve genes were identified as conferring resistance to erythromycin and tylosin (erm(B) and erm(C)), kanamycin (aph(3′)-IIIa), streptomycin (ant(6)-Ia), lincomycin (lnu(B)), Q/D (vat(E)), ciprofloxacin (qnrE. faecalis), and tetracycline (tet(K), tet(L), tet(M), tet(O) and tet(S)). Twelve different rep-families were identified in two-thirds of the isolates. While AR genes commonly found in human and animals were detected in this study among environmental enterococci, resistance genes could not be determined for many of the isolates, which indicates that diverse AR mechanisms exist among enterococci, and the understanding of AR mechanisms for environmental enterococci is limited. Diverse rep-families were identified among the enterococci recovered from the aquatic environment, and these rep-families appear to be quite different from those recovered from other sources. This work expands knowledge of AR gene reservoirs and enterococcal plasmids across a wider range of environments.
Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture
Authors: Salgueiro Vanessa , Manageiro Vera , Bandarra Narcisa M. , Reis Lígia , Ferreira Eugénia , Caniça Manuela
Published: September 2020

In a world where the population continues to increase and the volume of fishing catches stagnates or even falls, the aquaculture sector has great growth potential. This study aimed to contribute to the depth of knowledge of the diversity of bacterial species found in Sparus aurata collected from a fish farm and to understand which profiles of diminished susceptibility to antibiotics would be found in these bacteria that might be disseminated in the environment. One hundred thirty-six bacterial strains were recovered from the S. aurata samples. These strains belonged to Bacillaceae, Bacillales Family XII. Incertae Sedis, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Micrococcaceae, Pseudomonadaceae and Staphylococcaceae families. Enterobacter sp. was more frequently found in gills, intestine and skin groups than in muscle groups (p ≤ 0.01). Antibiotic susceptibility tests found that non-susceptibility to phenicols was significantly higher in gills, intestine and skin samples (45%) than in muscle samples (24%) (p ≤ 0.01) and was the most frequently found non-susceptibility in both groups of samples. The group of Enterobacteriaceae from muscles presented less decreased susceptibility to florfenicol (44%) than in the group of gills, intestine and skin samples (76%). We found decreased susceptibilities to β-lactams and glycopeptides in the Bacillaceae family, to quinolones and mupirocin in the Staphylococcaceae family, and mostly to β-lactams, phenicols and quinolones in the Enterobacteriaceae and Pseudomonadaceae families. Seven Enterobacter spp. and five Pseudomonas spp. strains showed non-susceptibility to ertapenem and meropenem, respectively, which is of concern because they are antibiotics used as a last resort in serious clinical infections. To our knowledge, this is the first description of species Exiguobacterium acetylicum, Klebsiella michiganensis, Lelliottia sp. and Pantoea vagans associated with S. aurata (excluding cases where these bacteria are used as probiotics) and of plasmid-mediated quinolone resistance qnrB19-producing Leclercia adecarboxylata strain. The non-synonymous G385T and C402A mutations at parC gene (within quinolone resistance-determining regions) were also identified in a Klebsiella pneumoniae, revealing decreased susceptibility to ciprofloxacin. In this study, we found not only bacteria from the natural microbiota of fish but also pathogenic bacteria associated with fish and humans. Several antibiotics for which decreased susceptibility was found here are integrated into the World Health Organization list of “critically important antimicrobials” and “highly important antimicrobials” for human medicine.
Probiotics, Prebiotics and Other Dietary Supplements for Gut Microbiota Modulation in Celiac Disease Patients
Authors: Marasco Giovanni , Cirota Giovanna Grazia , Rossini Benedetta , Lungaro Lisa , Di Biase Anna Rita , Colecchia Antonio , Volta Umberto , De Giorgio Roberto , Festi Davide , Caio Giacomo
Published: September 2020

To date, the only available treatment for celiac disease (CD) patients is a life-lasting gluten-free diet (GFD). Lack of adherence to the GFD leads to a significant risk of adverse health consequences. Food cross-contamination, nutritional imbalances, and persistent gastrointestinal symptoms are the main concerns related to GFD. Moreover, despite rigid compliance to GFD, patients struggle in achieving a full restoring of the gut microbiota, which plays a role in the nutritive compounds processing, and absorption. Pivotal studies on the supplementation of GFD with probiotics, such as Bifidobacterium and Lactobacilli, reported a potential to restore gut microbiota composition and to pre-digest gluten in the intestinal lumen, reducing the inflammation associated with gluten intake, the intestinal permeability, and the cytokine and antibody production. These findings could explain an improvement in symptoms and quality of life in patients treated with GFD and probiotics. On the other hand, the inclusion of prebiotics in GFD could also be easy to administer and cost-effective as an adjunctive treatment for CD, having the power to stimulate the growth of potentially health-promoting bacteria strains. However, evidence regarding the use of prebiotics and probiotics in patients with CD is still insufficient to justify their use in clinical practice.
Correction: Breakfast in Canada: Prevalence of Consumption, Contribution to Nutrient and Food Group Intakes, and Variability across Tertiles of Daily Diet Quality. A Study from the International Breakfast Research Initiative. Nutrients 2018, 10, 985
Authors: Barr Susan I. , Vatanparast Hassan , Smith Jessica
Published: September 2020

The authors wish to make a correction to the published version of their paper [...]