bluuebunny/crossref_metadata_2025 huggingface dataset:
Structural Characteristics of Crude Polysaccharides from 12 Selected Chinese Teas, and Their Antioxidant and Anti-Diabetic Activities
Authors: Guo Huan , Fu Meng-Xi , Wu Ding-Tao , Zhao Yun-Xuan , Li Hang , Li Hua-Bin , Gan Ren-You
Published: September 2021

Twelve representative edible Chinese teas (Camellia sinensis L.) from six categories (dark tea, black tea, oolong tea, white tea, yellow tea, and green tea) were selected in this study. Tea polysaccharides (TPs) were extracted with hot water, and their structural properties and biological activities, mainly antioxidant and anti-diabetic activities, were systematically evaluated. Results revealed that the extraction yields of TPs ranged from 1.81% to 6.38%, and Pu-erh tea polysaccharides had the highest extraction yield (6.38 ± 0.28%). The chemical compositions, molecular weight, and compositional monosaccharides of TPs varied among the six categories of tea. It appeared that all TPs were protein-bound acid heteropolysaccharides, and all TPs exhibited obvious antioxidant and anti-diabetic (e.g., α-glucosidase inhibitory and antiglycation) activities. Particularly, Pu-erh tea polysaccharides also contained the highest total phenolic and protein contents, and also exhibited the best antioxidant and anti-diabetic activities. Moreover, for the structural-function relationship, the heat map analysis found that total phenolic and protein contents in TPs were positively correlated with their antioxidant and anti-diabetic activities, indicating that the presence of phenolic compounds and proteins in the TPs might be the main contributors to their bioactivities. The conclusion from this study can help understand the structural-function relationship of crude tea polysaccharides.
Fusion of Unobtrusive Sensing Solutions for Home-Based Activity Recognition and Classification Using Data Mining Models and Methods
Authors: Ekerete Idongesit , Garcia-Constantino Matias , Konios Alexandros , Mustafa Mustafa A. , Diaz-Skeete Yohanca , Nugent Christopher , McLaughlin James
Published: September 2021

This paper proposes the fusion of Unobtrusive Sensing Solutions (USSs) for human Activity Recognition and Classification (ARC) in home environments. It also considers the use of data mining models and methods for cluster-based analysis of datasets obtained from the USSs. The ability to recognise and classify activities performed in home environments can help monitor health parameters in vulnerable individuals. This study addresses five principal concerns in ARC: (i) users’ privacy, (ii) wearability, (iii) data acquisition in a home environment, (iv) actual recognition of activities, and (v) classification of activities from single to multiple users. Timestamp information from contact sensors mounted at strategic locations in a kitchen environment helped obtain the time, location, and activity of 10 participants during the experiments. A total of 11,980 thermal blobs gleaned from privacy-friendly USSs such as ceiling and lateral thermal sensors were fused using data mining models and methods. Experimental results demonstrated cluster-based activity recognition, classification, and fusion of the datasets with an average regression coefficient of 0.95 for tested features and clusters. In addition, a pooled Mean accuracy of 96.5% was obtained using classification-by-clustering and statistical methods for models such as Neural Network, Support Vector Machine, K-Nearest Neighbour, and Stochastic Gradient Descent on Evaluation Test.
Use of a Negative-Pressure Wound Dressing to Prevent Surgical Site Complications after Revision Knee Arthroplasty—A Randomized Controlled Trial
Authors: Deborre Christoph , Ezissi Afoma , Jaenisch Max , Khoury Mona , Burger Christof , Randau Thomas Martin , Kohlhof Hendrik
Published: September 2021

The aim of this study was to determine if a negative pressure wound therapy (NPWT) system can facilitate wound healing and withstand mechanical stress owing to the high range of motion of the knee joint in total knee arthroplasty. The benefits of NPWT include aiding wound granulation and a reduction in wound edema and secretion; our goal is to investigate its benefits in primary wound closure. Within an eight-day duration of treatment, we compared standard wound dressing (gauze and compression) with the disposable NPWT system (PICO)TM. A total of 79 patients were recruited in a controlled, randomized, prospective, single-institution clinical trial. A total of 40 patients were allocated to the study group and 39 to the control group. In terms of blood loss and infection parameters, there was no significant difference between both groups. In the NPWT group, we determined a significant reduction in wound edema but none in wound secretion. While the NPWT dressings survived the mechanical effects of movement during postoperative physiotherapy, conventional compressive dressings were often renewed. Even though the risk factors that could lead to delayed wound healing were reduced in the test group, we could not identify any significant difference pertaining to delayed wound healing that required surgical treatment.
Radon Transform Based on Waveform for AVO-Preserving Data Construction
Authors: Wang Shengchao , Han Liguo , Gong Xiangbo , Zhang Pan
Published: September 2021

The traditional hyperbolic Radon transform suffers from the major problem of how to both obtain a high resolution and preserve the amplitude variation with offset (AVO). In the Radon domain, high resolution (sparseness) is a valid criterion. However, if a sparse model is obtained in the Radon domain due to averaging along the offset direction, then it is not possible to preserve the AVO in the inversion data. In addition, hyperbolic Radon transform has a time-variant kernel based on a traditional iterative algorithm, the conjugate gradient (CG), which requires significant computation time. To solve these problems, we propose a Radon transform based on waveform that contains both cycle and amplitude characteristics of seismic waves. The new transform entails creating an upper envelope for the seismic data and computing a preliminary forward Radon transform in the time domain. The forward Radon transform incorporates a priori information by measuring the energy of each slowness (p) trace to obtain the high-resolution result of the Radon domain. For AVO preserving, the proposed method uses polynomials to describe the AVO characteristics in the inverse Radon transform based on the least-squares inversion. Besides amplitude preserving and high resolution, the proposed method avoids using CG and greatly reduces the cost of computing hyperbolic Radon transform in the time domain. In applications to both synthetic and field data, waveform Radon transform (WRT) has a better performance than the conjugate gradient Radon transform (CGRT).
Development of Intellectual Web System for Morph Analyzing of Uzbek Words
Authors: Mengliev Davlatyor , Barakhnin Vladimir , Abdurakhmonova Nilufar
Published: September 2021

Currently, there is an active development of the Uzbek sector of the Internet. In it, as in other national sectors, the most common form of presentation of textual information is semi-structured documents, work that presupposes the availability of reliable algorithms for text analysis, including its lexical characteristics. The article offers an intelligent web application developed for morphological analysis of words in the Uzbek language. The web application is based on the concept of generation and stem analysis of the Uzbek language word forms. A well-known Porter algorithm was chosen as the basis for stemming. The morphoanalyzer generates word forms of the Uzbek language based on the division of words into certain classes, taking into account the specifics and structure of this language. For example, nouns can be classified by meaning (related, nominal), by quantity (singular and plural), by case, and also, by the endings of belonging (possessive).
Reduction of Dust on Solar Panels through Unipolar Electrostatic Traveling Wave
Authors: Altıntaş Murat , Arslan Serdal
Published: September 2021

In this study, a novel electrostatic cleaning scheme has been applied to a new designed and developed electrode having high cleaning efficiency. In this method, a high voltage, four-channel, 1 Hz square wave signal is applied to a specially designed electrode array. Models of the electric field distribution of the proposed electrode array were developed and analyzed using Ansys Maxwell simulation software and printed circuits boards (PCBs) were produced. The performance of dust removal using the electrodes was evaluated. A 1 MW solar power plant was taken as a case study, and performance and cost were compared with the classical panel cleaning method (wet cleaning system). Our study has shown that the electrostatic cleaning method can be carried out with lower cost and higher efficiency than existing methods.
Intramuscular Properties of Resting Lumbar Muscles in Patients with Unilateral Lower Limb Amputation
Authors: Chang Yunhee , Kang Jungsun , Kim Gyoosuk , Shin Hyunjun , Park Sehoon
Published: September 2021

Lower limb amputees (LLAs) have a high incidence of low back pain (LBP), and identifying the potential risk factors in this group is key for LBP prevention. This study analyzed the intramuscular properties of the resting lumbar muscle in thirteen unilateral LLAs and age-matched controls to predict the onset of LBP. To measure the lumbar intramuscular properties, resting erector spinae muscles located in the upper and lower lumbar regions were examined using a handheld myotonometer. The dynamic stiffness, oscillation frequency, and logarithmic decrement were measured. In our results, the stiffness and frequency of the upper lumbar region were greater in the amputee group than in the control, whereas the decrement did not differ between the two groups. Additionally, the measured values in the lower lumbar region showed no significant difference. Within each group, all three factors increased at the upper lumbar region. In the LLAs, the frequency and stiffness values of the upper lumbar on the non-amputated side were significantly higher than those on the amputated side. These results indicate that the upper lumbar muscles of the amputees were less flexible than that of the control. This study can help in providing therapeutic strategies treating LBP in amputees.
Calculation of the Lowest Resonant States of H− and Li by the Complex Absorbing Potential Method
Authors: Adamson Sergey O. , Kharlampidi Daria D. , Dyakov Yurii A. , Golubkov Gennady V. , Golubkov Maxim G.
Published: September 2021

The analysis of the features of the method of complex absorbing potential (CAP) is carried out for a single-channel problem with an explicit parameterization of the scattering matrix. It is shown that there can be several types of CAP trajectories depending on the choice of the initial conditions. In any case, the estimation of the resonance parameters from the position of the optimal trajectory point can lead to a systematic error or an ambiguous result. In special cases, the search for the optimal point can be replaced by the averaging over a closed section of the trajectory. The CAP trajectories constructed in the H− and Li resonance calculations correlate well with the model trajectories, which have a curl around the resonance. The averaging over a closed area of the trajectory leads to better estimates of the energy and width of the resonance in comparison with the technique of searching for the optimal point.
Immunostimulant Bathing Influences the Expression of Immune- and Metabolic-Related Genes in Atlantic Salmon Alevins
Authors: Figueiredo Filipe , Kristoffersen Harald , Bhat Shripathi , Zhang Zuobing , Godfroid Jacques , Peruzzi Stefano , Præbel Kim , Dalmo Roy Ambli , Xu Xiaoli
Published: September 2021

Disease resistance of fish larvae may be improved by bath treatment in water containing immunostimulants. Pattern recognition receptors, such as TLR3, TLR7, and MDA5, work as an “early warning” to induce intracellular signaling and facilitate an antiviral response. A single bath of newly hatched larvae, with Astragalus, upregulated the expression of IFNα, IFNc, ISG15, MDA5, PKR, STAT1, TLR3, and TLR7 immune genes, on day 4 post treatment. Similar patterns were observed for Hyaluronic acid and Poly I:C. Increased expression was observed for ISG15, MDA5, MX, STAT1, TLR3, TLR7, and RSAD2, on day 9 for Imiquimod. Metabolic gene expression was stimulated on day 1 after immunostimulant bath in ULK1, MYC, SLC2A1, HIF1A, MTOR, and SIX1, in Astragalus, Hyaluronic acid, and Imiquimod. Expression of NOS2 in Poly I:C was an average fourfold above that of control at the same timepoint. Throughout the remaining sampling days (2, 4, 9, 16, 32, and 45 days post immunostimulant bath), NOS2 and IL1B were consistently overexpressed. In conclusion, the immunostimulants induced antiviral gene responses, indicating that a single bath at an early life stage could enable a more robust antiviral defense in fish. Additionally, it was demonstrated, based on gene expression data, that cell metabolism was perturbed, where several metabolic genes were co-regulated with innate antiviral genes.
Disease Modeling of Mitochondrial Cardiomyopathy Using Patient-Specific Induced Pluripotent Stem Cells
Authors: Tokuyama Takeshi , Ahmed Razan Elfadil , Chanthra Nawin , Anzai Tatsuya , Uosaki Hideki
Published: September 2021

Mitochondrial cardiomyopathy (MCM) is characterized as an oxidative phosphorylation disorder of the heart. More than 100 genetic variants in nuclear or mitochondrial DNA have been associated with MCM. However, the underlying molecular mechanisms linking genetic variants to MCM are not fully understood due to the lack of appropriate cellular and animal models. Patient-specific induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iPSC-CMs) provide an attractive experimental platform for modeling cardiovascular diseases and predicting drug efficacy to such diseases. Here we introduce the pathological and therapeutic studies of MCM using iPSC-CMs and discuss the questions and latest strategies for research using iPSC-CMs.
Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm
Authors: Sharma Shivali , Schulthess Albert W. , Bassi Filippo M. , Badaeva Ekaterina D. , Neumann Kerstin , Graner Andreas , Özkan Hakan , Werner Peter , Knüpffer Helmut , Kilian Benjamin
Published: September 2021

Wheat (Triticum sp.) is one of the world’s most important crops, and constantly increasing its productivity is crucial to the livelihoods of millions of people. However, more than a century of intensive breeding and selection processes have eroded genetic diversity in the elite genepool, making new genetic gains difficult. Therefore, the need to introduce novel genetic diversity into modern wheat has become increasingly important. This review provides an overview of the plant genetic resources (PGR) available for wheat. We describe the most important taxonomic and phylogenetic relationships of these PGR to guide their use in wheat breeding. In addition, we present the status of the use of some of these resources in wheat breeding programs. We propose several introgression schemes that allow the transfer of qualitative and quantitative alleles from PGR into elite germplasm. With this in mind, we propose the use of a stage-gate approach to align the pre-breeding with main breeding programs to meet the needs of breeders, farmers, and end-users. Overall, this review provides a clear starting point to guide the introgression of useful alleles over the next decade.
Single-Cell RNA-Seq Reveals Heterogeneous lncRNA Expression in Xenografted Triple-Negative Breast Cancer Cells
Authors: Pinkney Holly R. , Black Michael A. , Diermeier Sarah D.
Published: September 2021

Breast cancer is the most commonly diagnosed cancer in the world, with triple-negative breast cancer (TNBC) making up 12% of these diagnoses. TNBC tumours are highly heterogeneous in both inter-tumour and intra-tumour gene expression profiles, where they form subclonal populations of varying levels of aggressiveness. These aspects make it difficult to study and treat TNBC, requiring further research into tumour heterogeneity as well as potential therapeutic targets and biomarkers. Recently, it was discovered that the majority of the transcribed genome comprises non-coding RNAs, in particular long non-coding RNAs (lncRNAs). LncRNAs are transcripts of >200 nucleotides in length that do not encode a protein. They have been characterised as regulatory molecules and their expression can be associated with a malignant phenotype. We set out to explore TNBC tumour heterogeneity in vivo at a single cell level to investigate whether lncRNA expression varies across different cells within the tumour, even if cells are coming from the same cell line, and whether lncRNA expression is sufficient to define cellular subpopulations. We applied single-cell expression profiling due to its ability to capture expression signals of lncRNAs expressed in small subpopulations of cells. Overall, we observed most lncRNAs to be expressed at low, but detectable levels in TNBC xenografts, with a median of 25 lncRNAs detected per cell. LncRNA expression alone was insufficient to define a subpopulation of cells, and lncRNAs showed highly heterogeneous expression patterns, including ubiquitous expression, subpopulation-specific expression, and a hybrid pattern of lncRNAs expressed in several, but not all subpopulations. These findings reinforce that transcriptionally defined tumour cell subpopulations can be identified in cell-line derived xenografts, and uses single-cell RNA-seq (scRNA-seq) to detect and characterise lncRNA expression across these subpopulations in xenografted tumours. Future studies will aim to investigate the spatial distribution of lncRNAs within xenografts and patient tissues, and study the potential of subclone-specific lncRNAs as new therapeutic targets and/or biomarkers.
Elastic Moduli of Avian Eggshell
Authors: Chiang Pei-Lin , Tseng Yu-Chien , Wu Hsiao-Jou , Tsao Shu-Han , Wu Shang-Ping , Wang Wei-Cheng , Hsieh Hsin-I , Juang Jia-Yang
Published: September 2021

We analyze 700 freshly-laid eggs from 58 species (22 families and 13 orders) across three orders of magnitude in egg mass. We study the elastic moduli using three metrics: (i) effective Young’s modulus, EFEM, by a combined experimental and numerical method; (ii) elastic modulus, Enano, by nanoindentation, and (iii) theoretical Young’s modulus, Etheory. We measure the mineral content by acid-base titration, and crystallographic characteristics by electron backscatter diffraction (EBSD), on representative species. We find that the mineral content ranges between 83.1% (Zebra finch) and 96.5% (ostrich) and is positively correlated with EFEM—23.28 GPa (Zebra finch) and 47.76 GPa (ostrich). The EBSD shows that eggshell is anisotropic and non-homogeneous, and different species have different degrees of crystal orientation and texture. Ostrich eggshell exhibits strong texture in the thickness direction, whereas chicken eggshell has little. Such anisotropy and inhomogeneity are consistent with the nanoindentation tests. However, the crystal characteristics do not appear to correlate with EFEM, as EFEM represents an overall “average” elasticity of the entire shell. The experimental results are consistent with the theoretical prediction of linear elasticity. Our comprehensive investigation into the elastic moduli of avian eggshell over broad taxonomic scales provides a useful dataset for those who work on avian reproduction.
The Coexistence of Blastocystis spp. in Humans, Animals and Environmental Sources from 2010–2021 in Asia
Authors: Rauff-Adedotun Adedolapo Aminat , Meor Termizi Farah Haziqah , Shaari Nurshafarina , Lee Ii Li
Published: September 2021

Blastocystis spp. are controversial unicellular protists that inhabit the gastrointestinal tract of humans and a wide range of animals worldwide. This review provides an overview of the prevalence and distribution of Blastocystis spp. and their subtypes throughout Asia. Research articles reporting on the presence of Blastocystis spp. in locations within Asia, between 1 January 2010, and 10 May 2021, were obtained from Scopus, PubMed, and Google Scholar. In 427 articles, the prevalence of Blastocystis spp. in 31 countries within the last decade was revealed. Isolates were found in humans, various mammals, birds, reptiles, insects, water sources, vegetables, and ambient air. Prevalence of Blastocystis spp. varied widely across host categories. Subtypes identified throughout Asia were STs 1–14, and ST18–22 (novel subtypes). ST1, ST2, ST3, ST4 were the most frequently isolated in humans; ST5 in pigs; ST10 and ST14 in goats, sheep, and cattle; and ST6 and ST7 in chickens. ST1 and ST3 were most common in water samples. ST1, ST2, ST3, ST4, ST5 and ST6 were shared by humans, animals, and water sources. There is a growing interest in the study of Blastocystis spp. and their subtypes in Asia. Due to the isolation of Blastocystis spp. from biotic and abiotic sources in Asia, the application of the One Health (OH) approach to the study of Blastocystis spp. is proposed for improved perception of this organism.
Circulating MicroRNAs as Novel Potential Diagnostic Biomarkers for Osteosarcoma: A Systematic Review
Authors: Gally Thaís Borges , Aleluia Milena Magalhães , Borges Grasiely Faccin , Kaneto Carla Martins
Published: September 2021

Osteosarcoma (OS) is a fast-progressing bone tumor with high incidence in children and adolescents. The main diagnostic methods for OS are imaging exams and biopsies. In spite of the several resources available for detecting the disease, establishing an early diagnosis is still difficult, resulting in worse prognosis and lower survival rates for patients with OS. The identification of novel biomarkers would be helpful, and recently, circulating microRNAs (miRNAs) have been pointed to as possible non-invasive biomarkers. In order to assess the effectiveness of miRNA research, we performed a systematic review to assess the potential role of circulating miRNAs as biomarkers for OS diagnosis. We performed a search in various databases—PubMed, LILACS (Literatura Latino-americana e do Caribe em Ciências da Saúde), VHL (Virtual Health Library), Elsevier, Web of Science, Gale Academic One File—using the terms: “Circulating microRNAs” OR “plasma microRNAs” OR “serum microRNAs” OR “blood microRNAs” OR “cell-free microRNAs” OR “exosome microRNAs” OR “extracellular vesicles microRNAs” OR “liquid biopsy” AND “osteosarcoma” AND “diagnostic”. We found 35 eligible studies that were independently identified and had had their quality assessed according to Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) guidelines. Despite the useful number of publications on this subject and the fact that several microRNAs showed excellent diagnostic performance for OS, the lack of consistency in results suggests that additional prospective studies are needed to confirm the role of circulating miRNAs as non-invasive biomarkers in OS.
No Effect of Hypercholesterolemia on Elastase-Induced Experimental Abdominal Aortic Aneurysm Progression
Authors: Ikezoe Toru , Shoji Takahiro , Guo Jia , Shen Fanru , Lu Hong S. , Daugherty Alan , Nunokawa Masao , Kubota Hiroshi , Miyata Masaaki , Xu Baohui , Dalman Ronald L.
Published: September 2021

Objective: Epidemiological studies link hyperlipidemia with increased risk for abdominal aortic aneurysms (AAAs). However, the influence of lipid-lowering drugs statins on prevalence and progression of clinical and experimental AAAs varies between reports, engendering controversy on the association of hyperlipidemia with AAA disease. This study investigated the impact of hypercholesterolemia on elastase-induced experimental AAAs in mice. Methods: Both spontaneous (targeted deletion of apolipoprotein E) and induced mouse hypercholesterolemia models were employed. In male wild type (WT) C57BL/6J mice, hypercholesterolemia was induced via intraperitoneal injection of an adeno-associated virus (AAV) encoding a gain-of-function proprotein convertase subtilisin/kexin type 9 mutation (PCSK9) followed by the administration of a high-fat diet (HFD) (PCSK9+HFD) for two weeks. As normocholesterolemic controls for PCSK9+HFD mice, WT mice were infected with PCSK9 AAV and fed normal chow, or injected with phosphate-buffered saline alone and fed HFD chow. AAAs were induced in all mice by intra-aortic infusion of porcine pancreatic elastase and assessed by ultrasonography and histopathology. Results: In spontaneous hyper- and normo-cholesterolemic male mice, the aortic diameter enlarged at a constant rate from day 3 through day 14 following elastase infusion. AAAs, defined as a more than 50% diameter increase over baseline measurements, formed in all mice. AAA progression was more pronounced in male mice, with or without spontaneous hyperlipidemia. The extent of elastin degradation and smooth muscle cell depletion were similar in spontaneous hyper- (score 3.5 for elastin and 4.0 for smooth muscle) and normo- (both scores 4.0) cholesterolemic male mice. Aortic mural macrophage accumulation was also equivalent between the two groups. No differences were observed in aortic accumulation of CD4+ or CD8+ T cells, B cells, or mural angiogenesis between male spontaneous hyper- and normocholesterolemic mice. Similarly, no influence of spontaneous hypercholesterolemia on characteristic aneurysmal histopathology was noted in female mice. In confirmatory experiments, induced hypercholesterolemia also exerted no appreciable effect on AAA progression and histopathologies. Conclusion: This study demonstrated no recognizable impact of hypercholesterolemia on elastase-induced experimental AAA progression in both spontaneous and induced hypercholesterolemia mouse models. These results add further uncertainty to the controversy surrounding the efficacy of statin therapy in clinical AAA disease.
Mevalonate Kinase Deficiency and Squalene Synthase Inhibitor (TAK-475): The Balance to Extinguish the Inflammation
Authors: Rimondi Erika , Valencic Erica , Tommasini Alberto , Secchiero Paola , Melloni Elisabetta , Marcuzzi Annalisa
Published: September 2021

Mevalonate Kinase Deficiency (MKD) is a rare inborn disease belonging to the family of periodic fever syndromes. The MKD phenotype is characterized by systemic inflammation involving multiple organs, including the nervous system. Current anti-inflammatory approaches to MKD are only partially effective and do not act specifically on neural inflammation. According to the new emerging pharmacology trends, the repositioning of drugs from the indication for which they were originally intended to another one can make mechanistic-based medications easily available to treat rare diseases. According to this perspective, the squalene synthase inhibitor Lapaquistat (TAK-475), originally developed as a cholesterol-lowering drug, might find a new indication in MKD, by modulating the mevalonate cholesterol pathway, increasing the availability of anti-inflammatory isoprenoid intermediates. Using an in vitro model for MKD, we mimicked the blockade of the cholesterol pathway and evaluated the potential anti-inflammatory effect of Lapaquistat. The results obtained showed anti-inflammatory effects of Lapaquistat in association with a low blockade of the metabolic pathway, while this effect did not remain with a tighter blockade. On these bases, Lapaquistat could be configured as an effective treatment for MKD’s mild forms, in which the residual enzymatic activity is only reduced and not almost completely absent as in the severe forms.
Reno-Protective Effect of GLP-1 Receptor Agonists in Type1 Diabetes: Dual Action on TRPC6 and NADPH Oxidases
Authors: Youssef Natalie , Noureldein Mohamed , Njeim Rachel , Ghadieh Hilda , Harb Frederic , Azar Sami , Fares Nassim , Eid Assaad
Published: September 2021

Diabetic kidney disease (DKD), a serious diabetic complication, results in podocyte loss and proteinuria through NADPH oxidases (NOX)-mediated ROS production. DUOX1 and 2 are NOX enzymes that require calcium for their activation which enters renal cells through the pivotal TRPC channels. Hypoglycemic drugs such as liraglutide can interfere with this deleterious mechanism imparting reno-protection. Herein, we aim to investigate the reno-protective effect of GLP1 receptor agonist (GLP1-RA), via its effect on TRPC6 and NADPH oxidases. To achieve our aim, control or STZ-induced T1DM Sprague–Dawley rats were used. Rats were treated with liraglutide, metformin, or their combination. Functional, histological, and molecular parameters of the kidneys were assessed. Our results show that treatment with liraglutide, metformin or their combination ameliorates DKD by rectifying renal function tests and protecting against fibrosis paralleled by restored mRNA levels of nephrin, DUOX1 and 2, and reduced ROS production. Treatment with liraglutide reduces TRPC6 expression, while metformin treatment shows no effect. Furthermore, TRPC6 was found to be directly interacting with nephrin, and indirectly interacting with DUOX1, DUOX2 and GLP1-R. Our findings suggest that treatment with liraglutide may prevent the progression of diabetic nephropathy by modulating the crosstalk between TRPC6 and NADPH oxidases.
Surface Plasmon Resonance for Protease Detection by Integration of Homogeneous Reaction
Authors: Xia Ning , Liu Gang , Yi Xinyao
Published: September 2021

The heterogeneous assays of proteases usually require the immobilization of peptide substrates on the solid surface for enzymatic hydrolysis reactions. However, immobilization of peptides on the solid surface may cause a steric hindrance to prevent the interaction between the substrate and the active center of protease, thus limiting the enzymatic cleavage of the peptide. In this work, we reported a heterogeneous surface plasmon resonance (SPR) method for protease detection by integration of homogeneous reaction. The sensitivity was enhanced by the signal amplification of streptavidin (SA)-conjugated immunoglobulin G (SA-IgG). Caspase-3 (Cas-3) was determined as the model. A peptide labeled with two biotin tags at the N- and C-terminals (bio-GDEVDGK-bio) was used as the substrate. In the absence of Cas-3, the substrate peptide was captured by neutravidin (NA)-covered SPR chip to facilitate the attachment of SA-IgG by the avidin-biotin interaction. However, once the peptide substrate was digested by Cas-3 in the aqueous phase, the products of bio-GDEVD and GK-bio would compete with the substrate to bond NA on the chip surface, thus limiting the attachment of SA-IgG. The method integrated the advantages of both heterogeneous and homogeneous assays and has been used to determine Cas-3 inhibitor and evaluate cell apoptosis with satisfactory results.
An Optical Smartphone-Based Inspection Platform for Identification of Diseased Orchids
Authors: Lee Kuan-Chieh , Wang Yen-Hsiang , Wei Wen-Chun , Chiang Ming-Hsien , Dai Ting-En , Pan Chung-Cheng , Chen Ting-Yuan , Luo Shi-Kai , Li Po-Kuan , Chen Ju-Kai , Liaw Shien-Kuei , Lin Choa-Feng , Wu Chin-Cheng , Chieh Jen-Jie
Published: September 2021

Infections of orchids by the Odontoglossum ringspot virus or Cymbidium mosaic virus cause orchid disfiguration and are a substantial source of economic loss for orchid farms. Although immunoassays can identify these infections, immunoassays are expensive, time consuming, and labor consuming and limited to sampling-based testing methods. This study proposes a noncontact inspection platform that uses a spectrometer and Android smartphone. When orchid leaves are illuminated with a handheld optical probe, the Android app based on the Internet of Things and artificial intelligence can display the measured florescence spectrum and determine the infection status within 3 s by using an algorithm hosted on a remote server. The algorithm was trained on optical data and the results of polymerase chain reaction assays. The testing accuracy of the algorithm was 89%. The area under the receiver operating characteristic curve was 91%; thus, the platform with the algorithm was accurate and convenient for infection screening in orchids.
Six Flux Model for the Central Lamp Reactor Applied to an External Four-Lamp Reactor
Authors: Beltrán Fernando J. , Rivas Javier , Garcia-Araya Juan-Fernando
Published: September 2021

One of the difficulties of establishing the intrinsic kinetics of photocatalytic oxidation processes is due to the complex mathematical formula used to determine the rate of photon absorption. To solve this problem, some models have been proposed and checked, such as the Six Flux Model (SFM) confirmed in central lamp photoreactors. External lamp photoreactors are also one of the most used configurations to study the photocatalytic oxidation of contaminants in water, and complex mathematical solutions have been reported to solve the rate of photon absorption. In this work, SFM Equations already reported for the central lamp photoreactor have been adapted to determine the rate of photon absorption in an external four-lamp photoreactor. The results obtained show slight differences from those of the Monte Carlo method. Additionally, once the rate of photon absorption was validated, the intrinsic rate constant and scavenging factor of the photocatalytic oxidation of some contaminant compounds from results already published have been determined.
Constitutive Expression in Komagataella phaffii of Mature Rhizopus oryzae Lipase Jointly with Its Truncated Prosequence Improves Production and the Biocatalyst Operational Stability
Authors: López-Fernández Josu , Benaiges Maria Dolors , Valero Francisco
Published: September 2021

Rhizopus oryzae lipase (ROL) containing 28 C-terminal amino acids of the prosequence fused to the N-terminal mature sequence in ROL (proROL) was successfully expressed in the methylotrophic yeast Komagataella phaffii (Pichia pastoris) under the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP). Although the sequence encoding the mature lipase (rROL) was also transformed, no clones were obtained after three transformation cycles, which highlights the importance of the truncated prosequence to obtain viable transformed clones. Batch cultures of the K. phaffii strain constitutively expressing proROL scarcely influenced growth rate and exhibited a final activity and volumetric productivity more than six times higher than those obtained with proROL from K. phaffii under the methanol-inducible alcohol oxidase 1 promoter (PAOX1). The previous differences were less marked in fed-batch cultures. N-terminal analysis confirmed the presence of the 28 amino acids in proROL. In addition, immobilized proROL exhibited increased tolerance of organic solvents and an operational stability 0.25 and 3 times higher than that of immobilized rROL in biodiesel and ethyl butyrate production, respectively. Therefore, the truncated prosequence enables constitutive proROL production, boosts bioprocess performance and provides a more stable biocatalyst in two reactions in which lipases are mostly used at industrial level, esterification (ethyl butyrate) and transesterification (biodiesel).
Cutinases: Characteristics and Insights in Industrial Production
Authors: Martínez Alejandro , Maicas Sergi
Published: September 2021

Cutinases (EC 3.1.1.74) are serin esterases that belong to the α/β hydrolases superfamily and present in the Ser-His-Asp catalytic triad. They show characteristics between esterases and lipases. These enzymes hydrolyze esters and triacylglycerols and catalyze esterification and transesterification reactions. Cutinases are synthesize by plant pathogenic fungi, but some bacteria and plants have been found to produce cutinases as well. In nature they facilitate a pathogen’s invasion by hydrolyzing the cuticle that protects plants, but can be also used for saprophytic fungi as a way to nourish themselves. Cutinases can hydrolyze a wide range of substrates like esters, polyesters, triacylglycerols and waxes and that makes this enzyme very attractive for industrial purposes. This work discusses techniques of industrial interest such as immobilization and purification, as well as some of the most important uses of cutinases in industries.
Enzymatic Hydrogen Electrosynthesis at Enhanced Current Density Using a Redox Polymer
Authors: Ruth John C. , Schwarz Fabian M. , Müller Volker , Spormann Alfred M.
Published: September 2021

High-temperature tolerant enzymes offer multiple advantages over enzymes from mesophilic organisms for the industrial production of sustainable chemicals due to high specific activities and stabilities towards fluctuations in pH, heat, and organic solvents. The production of molecular hydrogen (H2) is of particular interest because of the multiple uses of hydrogen in energy and chemicals applications, and the ability of hydrogenase enzymes to reduce protons to H2 at a cathode. We examined the activity of Hydrogen-Dependent CO2 Reductase (HDCR) from the thermophilic bacterium Thermoanaerobacter kivui when immobilized in a redox polymer, cobaltocene-functionalized polyallylamine (Cc-PAA), on a cathode for enzyme-mediated H2 formation from electricity. The presence of Cc-PAA increased reductive current density 340-fold when used on an electrode with HDCR at 40 °C, reaching unprecedented current densities of up to 3 mA·cm−2 with minimal overpotential and high faradaic efficiency. In contrast to other hydrogenases, T. kivui HDCR showed substantial reversibility of CO-dependent inactivation, revealing an opportunity for usage in gas mixtures containing CO, such as syngas. This study highlights the important potential of combining redox polymers with novel enzymes from thermophiles for enhanced electrosynthesis.
Distinct Clinical Impact and Biological Function of Angiopoietin and Angiopoietin-like Proteins in Human Breast Cancer
Authors: Yang Hui , Zhang Melody , Mao Xuan-Yu , Chang Hang , Perez-Losada Jesus , Mao Jian-Hua
Published: September 2021

Secreted angiopoietin/angiopoietin-like (ANGPT/ANGPTL) proteins are involved in many biological processes. However, the role of these proteins in human breast cancers (BCs) remains largely unclear. Here, we conducted integrated omics analyses to evaluate the clinical impact of ANGPT/ANGPTL proteins and to elucidate their biological functions. In BCs, we identified rare mutations in ANGPT/ANGPTL genes, frequent gains of ANGPT1, ANGPT4, and ANGPTL1, and frequent losses of ANGPT2, ANGPTL5, and ANGPTL7, but observed that ANGPTL1, 2, and 4 were robustly downregulated in multiple datasets. The expression levels of ANGPTL1, 5, and 8 were positively correlated with overall survival (OS), while the expression levels of ANGPTL4 were negatively correlated with OS. Additionally, the expression levels of ANGPTL1 and 7 were positively correlated with distant metastasis-free survival (DMFS), while the expression levels of ANGPT2 and ANGPTL4 were negatively correlated with DMFS. The prognostic impacts of ANGPT/ANGPTL genes depended on the molecular subtypes and on clinical factors. We discovered that various ANGPT/ANGPTL genes were co-expressed with various genes involved in different pathways. Finally, with the exception of ANGPTL3, the remaining genes showed significant correlations with cancer-associated fibroblasts, endothelial cells, and microenvironment score, whereas only ANGPTL6 was significantly correlated with immune score. Our findings provide strong evidence for the distinct clinical impact and biological function of ANGPT/ANGPTL proteins, but the question of whether some of them could be potential therapeutic targets still needs further investigation in BCs.
New Insights into Intestinal Permeability in Irritable Bowel Syndrome-Like Disorders: Histological and Ultrastructural Findings of Duodenal Biopsies
Authors: Miglietta Selenia , Borghini Raffaele , Relucenti Michela , Sorrentino Veronica , Chen Rui , Li Xiaobo , Fazi Francesco , Donato Giuseppe , Familiari Giuseppe , Petrozza Vincenzo , Picarelli Antonio
Published: September 2021

Background and Aim: Diarrhea, abdominal pain, and bloating are frequent in irritable bowel syndrome (IBS)-like disorders, although little is known about their intestinal ultrastructural alterations. The aim of the present study was to study duodenal biopsies from IBS-like patients to find ultrastructural alterations. Materials and Methods: Study design: descriptive comparative pilot study. Thirty outpatients (9 male and 21 female; median age 37.7 years; range, 20 to 65 years) complaining of IBS-like symptoms were enrolled between January 2015 to May 2019 and were divided into 6 groups, each equally consisting of 5 patients: (A) untreated celiac disease (uCD); (B) treated celiac disease (tCD); (C) wheat allergy (WA); (D) Non-celiac gluten sensitivity (NCGS); (E) Nickel allergic contact mucositis (Ni ACM); (F) controls affected by GERD. Transmission electron microscopy (TEM) morphological characteristics were: microvilli length, intermicrovillar distance, junctional complexes (JC) gap width, autophagic bodies, apoptosis, altered mitochondria, lipid/chylomicron droplets, and mast cells. Regarding JC, we focused on tight junctions (TJ), adherens junctions (AJ), and desmosomes. Results: Major alterations in microvilli length and intermicrovillar distance have been observed in the subjects affected by uCD. Microvilli of tCD patients showed marked recovery after adequate GFD, although not comparable to controls. Intermediate microvillar alterations were instead observed in NCGS and Ni ACM, while characteristics of WA subjects appeared more similar to tCD. Regarding JC, TJ did not show significant differences between all groups studied, including controls. The AJ were significantly more dilated in all groups compared to controls, while no significant differences were found between the pathological groups. The distance between desmosomes was greater in uCD, NCGS, and Ni ACM than in tCD, WA, and controls. Finally, intracellular alterations have been detected in most of the groups studied although they seemed more unspecific. Conclusions: TEM analysis confirmed damages to the intestinal barrier and defense mechanisms by enterocytes in IBS-like patients, probably linked to low-grade inflammation or adverse reactions triggered by food allergens, heavy metals, or other unknown. On the other hand, our study needs confirmation and further investigations with larger populations to facilitate diagnosis, therapy, and prevention of IBS-like disorders in the future.
The Coordinated KNR6–AGAP–ARF1 Complex Modulates Vegetative and Reproductive Traits by Participating in Vesicle Trafficking in Maize
Authors: Li Manfei , Zhao Ran , Du Yanfang , Shen Xiaomeng , Ning Qiang , Li Yunfu , Liu Dan , Xiong Qing , Zhang Zuxin
Published: September 2021

The KERNEL NUMBER PER ROW6 (KNR6)-mediated phosphorylation of an adenosine diphosphate ribosylation factor (Arf) GTPase-activating protein (AGAP) forms a key regulatory module for the numbers of spikelets and kernels in the ear inflorescences of maize (Zea mays L.). However, the action mechanism of the KNR6–AGAP module remains poorly understood. Here, we characterized the AGAP-recruited complex and its roles in maize cellular physiology and agronomically important traits. AGAP and its two interacting Arf GTPase1 (ARF1) members preferentially localized to the Golgi apparatus. The loss-of-function AGAP mutant produced by CRISPR/Cas9 resulted in defective Golgi apparatus with thin and compact cisternae, together with delayed internalization and repressed vesicle agglomeration, leading to defective inflorescences and roots, and dwarfed plants with small leaves. The weak agap mutant was phenotypically similar to knr6, showing short ears with fewer kernels. AGAP interacted with KNR6, and a double mutant produced shorter inflorescence meristems and mature ears than the single agap and knr6 mutants. We hypothesized that the coordinated KNR6–AGAP–ARF1 complex modulates vegetative and reproductive traits by participating in vesicle trafficking in maize. Our findings provide a novel mechanistic insight into the regulation of inflorescence development, and ear length and kernel number, in maize.
Adipose Tissue Steroid Receptor RNA Activator 1 (SRA1) Expression Is Associated with Obesity, Insulin Resistance, and Inflammation
Authors: Kochumon Shihab , Arefanian Hossein , Sindhu Sardar , Shenouda Steve , Thomas Reeby , Al-Mulla Fahd , Tuomilehto Jaakko , Ahmad Rasheed
Published: September 2021

Steroid receptor RNA activator 1 (SRA1) is involved in pathophysiological responses of adipose tissue (AT) in obesity. In vitro and animal studies have elucidated its role in meta-inflammation. Since SRA1 AT expression in obesity/type 2 diabetes (T2D) and the relationship with immune-metabolic signatures remains unclear, we assessed AT SRA1 expression and its association with immune–metabolic markers in individuals with obesity/T2D. For this, 55 non-diabetic and 53 T2D individuals classified as normal weight (NW; lean), overweight, and obese were recruited and fasting blood and subcutaneous fat biopsy samples were collected. Plasma metabolic markers were assessed using commercial kits and AT expression of SRA1 and selected immune markers using RT-qPCR. SRA1 expression was significantly higher in non-diabetic obese compared with NW individuals. SRA1 expression associated with BMI, PBF, serum insulin, and HOMA-IR in the total study population and people without diabetes. SRA1 associated with waist circumference in people without diabetes and NW participants, whereas it associated inversely with HbA1c in overweight participants. In most study subgroups AT SRA1 expression associated directly with CXCL9, CXCL10, CXCL11, TNF-α, TGF-β, IL2RA, and IL18, but inversely with CCL19 and CCR2. TGF-β/IL18 independently predicted the SRA1 expression in people without diabetes and in the total study population, while TNF-α/IL-2RA predicted SRA1 only in people with diabetes. TNF-α also predicted SRA1 in both NW and obese people regardless of the diabetes status. In conclusion, AT SRA1 expression is elevated in people with obesity which associates with typical immunometabolic markers of obesity/T2D, implying that SRA1 may have potential as a biomarker of metabolic derangements.
Pollution Flashover Characteristics of Coated Insulators under Different Profiles of Coating Damage
Authors: Salem Ali Ahmed , Lau Kwan Yiew , Rahiman Wan , Al-Gailani Samir A. , Abdul-Malek Zulkurnain , Abd Rahman Rahisham , Al-Ameri Salem Mgammal , Sheikh Usman Ullah
Published: September 2021

Based on experiments and numerical analysis techniques, this paper aims to investigate the influence of the four different coating damage profiles on the performance of coated 33 kV porcelain insulator strings under polluted and clean surface conditions. The performance of the insulators coated with room temperature vulcanizing (RTV) under partial coating damage and undamaged coating was evaluated. The influence of humidity on pollution flashover was taken into consideration. The ring-shaped, fan-shaped, and random-shaped coating was applied following coating damage. The results showed that the flashover characteristic of the RTV-coated insulators had a significant difference as compared to the normal insulators. Electrical characteristics such as the flashover voltage, critical current, and surface resistance were significantly affected by coating damage distribution and humidity level on the insulators’ surface. The electric field and potential difference were analyzed as well using the finite element method (FEM). The initiation of the arc was observed to appear at the area of insulators where the electric field was the highest. It was also observed that different coating distributions of pollution and humidity levels resulted in a change in the surface pollution layer resistance and an uneven distribution of the electric field. This indicates that the coated insulators’ parameters are directly related to the coating damage distribution on the insulator surface, particularly in the presence of humidity.