bluuebunny/crossref_metadata_2025 huggingface dataset:
Recognition of commensal bacterial peptidoglycans defines Drosophila gut homeostasis and lifespan
Authors: Onuma Taro , Yamauchi Toshitaka , Kosakamoto Hina , Kadoguchi Hibiki , Kuraishi Takayuki , Murakami Takumi , Mori Hiroshi , Miura Masayuki , Obata Fumiaki
Published: April 2023

Commensal microbes in animals have a profound impact on tissue homeostasis, stress resistance, and ageing. We previously showed in Drosophila melanogaster that Acetobacter persici is a member of the gut microbiota that promotes ageing and shortens fly lifespan. However, the molecular mechanism by which this specific bacterial species changes lifespan and physiology remains unclear. The difficulty in studying longevity using gnotobiotic flies is the high risk of contamination during ageing. To overcome this technical challenge, we used a bacteria-conditioned diet enriched with bacterial products and cell wall components. Here, we demonstrate that an A. persici-conditioned diet shortens lifespan and increases intestinal stem cell (ISC) proliferation. Feeding adult flies a diet conditioned with A. persici, but not with Lactiplantibacillus plantarum, can decrease lifespan but increase resistance to paraquat or oral infection of Pseudomonas entomophila, indicating that the bacterium alters the trade-off between lifespan and host defence. A transcriptomic analysis using fly intestine revealed that A. persici preferably induces antimicrobial peptides (AMPs), while L. plantarum upregulates amidase peptidoglycan recognition proteins (PGRPs). The specific induction of these Imd target genes by peptidoglycans from two bacterial species is due to the stimulation of the receptor PGRP-LC in the anterior midgut for AMPs or PGRP-LE from the posterior midgut for amidase PGRPs. Heat-killed A. persici also shortens lifespan and increases ISC proliferation via PGRP-LC, but it is not sufficient to alter the stress resistance. Our study emphasizes the significance of peptidoglycan specificity in determining the gut bacterial impact on healthspan. It also unveils the postbiotic effect of specific gut bacterial species, which turns flies into a "live fast, die young" lifestyle.
Comparison of toxic heavy metals in the breast milk of diabetic and non-diabetic postpartum mothers in Yenagoa, Nigeria
Authors: Philip-Slaboh Tuboseiyefah Perekebi , Eleke Chinemerem , Ezejiofor Anthonet Ndidiamaka
Published: April 2023

Background

Breast milk is the main source of neonatal nutrition. It is not known whether diabetes increases the excretion of toxic heavy metals in the breast milk of postpartum mothers. We compared the concentration of toxic heavy metals in breast milk between diabetic and non-diabetic postpartum mothers in Yenagoa.

Material and methods

A cross-sectional design was utilized on a purposive sample of 144 consenting postpartum mothers (72 diabetic and 72 non-diabetic mothers) from three public hospitals. Breast milk samples were collected at 5–6 weeks postpartum between 1st November 2020 and 30th April 2021. Atomic-Absorption-Spectrophotometer and Direct-Mercury-Analyzer were used to analyze the breast milk samples. A data collection form (proforma) was used and data were analyzed at a 5% significance level with IBM-SPSS 25 software.

Result

High levels of Arsenic (63.9% vs. 62.5%), Lead (95.8% vs. 95.8%), Mercury (68.1% vs. 72.2%), and Cadmium (84.7% vs. 86.1%) were detected in the breast milk of the diabetic and non-diabetic groups respectively. The mean concentrations for Arsenic (0.6 vs. 0.6 ng/mL), Lead (13.2 vs. 12.2 ng/mL), Mercury (2.9 vs. 3.0 ng/mL), and Cadmium (3.3 vs. 3.2 ng/mL) were above the WHO permissible limits, thus showing evidence of risk to the health of the mother and neonate. There was no significant difference in the concentration of toxic heavy metals in breast milk between the groups (p = > 0.585).

Conclusions

Diabetes did not seem to increase the concentration of toxic heavy metals expressed in breast milk. More rigorous studies are needed to confirm these findings.
Metastatic colorectal adenocarcinoma tumor purity assessment from whole exome sequencing data
Authors: Tbeileh Noura , Timmerman Luika , Mattis Aras N. , Toriguchi Kan , Kasai Yosuke , Corvera Carlos , Nakakura Eric , Hirose Kenzo , Donner David B. , Warren Robert S. , Karelehto Eveliina
Published: April 2023

Tumors rich in stroma are associated with advanced stage and poor prognosis in colorectal adenocarcinoma (CRC). Abundance of stromal cells also has implications for genomic analysis of patient tumors as it may prevent detection of somatic mutations. As part of our efforts to interrogate stroma-cancer cell interactions and to identify actionable therapeutic targets in metastatic CRC, we aimed to determine the proportion of stroma embedded in hepatic CRC metastases by performing computational tumor purity analysis based on whole exome sequencing data (WES). Unlike previous studies focusing on histopathologically prescreened samples, we used an unbiased in-house collection of tumor specimens. WES from CRC liver metastasis samples were utilized to evaluate stromal content and to assess the performance of three in silico tumor purity tools, ABSOLUTE, Sequenza and PureCN. Matching tumor derived organoids were analyzed as a high purity control as they are enriched in cancer cells. Computational purity estimates were compared to those from a histopathological assessment conducted by a board-certified pathologist. According to all computational methods, metastatic specimens had a median tumor purity of 30% whereas the organoids were enriched for cancer cells with a median purity estimate of 94%. In line with this, variant allele frequencies (VAFs) of oncogenes and tumor suppressor genes were undetectable or low in most patient tumors, but higher in matching organoid cultures. Positive correlation was observed between VAFs and in silico tumor purity estimates. Sequenza and PureCN produced concordant results whereas ABSOLUTE yielded lower purity estimates for all samples. Our data shows that unbiased sample selection combined with molecular, computational, and histopathological tumor purity assessment is critical to determine the level of stroma embedded in metastatic colorectal adenocarcinoma.
Methylation of the glucocorticoid receptor gene (NR3C1) in dyads mother-child exposed to intimate partner violence in Cameroon: Association with anxiety symptoms
Authors: Wadji Dany Laure , Morina Naser , Martin-Soelch Chantal , Wicky Chantal
Published: April 2023

Background

The glucocorticoid receptor (GR), which is encoded by the NR3C1 (Nuclear Receptor Subfamily 3 Group C Member 1) gene plays an important role in the modulation of the hypothalamic-pituitary-adrenal (HPA) axis activity by providing feedback regulation which allows termination of the stress response. Little is known about epigenetic programming at the level of NGFI-A (nerve growth factor-inducible protein A) putative binding site (CpG) of the NR3C1 exon 1F in dyads mother-child exposed to intimate partner violence (IPV) more specifically in an unstudied region such as the sub-Saharan Africa where levels of violence are very high.

Objective

Examine NR3C1 exon 1F methylation in response to IPV and possible association with cortisol concentration and mental health.

Method

We recruited 20 mother–child dyads exposed to IPV and a control group of 20 mother–child dyads not exposed to IPV. We administered self-reported questionnaires to measure mother’s mental health and collected saliva samples for cortisol dosage and bisulfite sequencing of DNA methylation.

Results

Regarding the mothers, our results showed a significant difference in methylation level at CpG 16–21 sites of the NR3C1 exon 1F promoter region between the groups. In the exposed group as compared to the control group, there was a significant positive association between the level of methylation at CpG 16–21 sites and mother’s mental health in particular anxiety symptoms. However, we did not find any significant correlation between methylation level and cortisol concentration. In children, we did not find any significant results.

Conclusion

This study highlights a NGFI-A putative binding site (CpG 16–21) that is more methylated in mothers exposed to IPV and which may have the potential to confer vulnerability for psychopathologies.
Prevalence of dental caries and associated risk factors among People Living with HIV/AIDS and HIV uninfected adults at an HIV clinic in Kigali, Rwanda
Authors: Murererehe Julienne , Malele-Kolisa Yolanda , Niragire François , Yengopal Veerasamy
Published: April 2023

Background

Dental caries is among the most frequent oral conditions in People Living with HIV/AIDS (PLWHA). There is a lack of baseline information on dental caries prevalence and associated risk factors among PLWHA in comparison to HIV uninfected people in Rwanda.

Objective

This study was conducted to determine the prevalence of dental caries and associated risk factors among PLWHA and HIV uninfected adults at an HIV clinic of Kigali Teaching Hospital (CHUK) in Kigali, Rwanda.

Methods

A comparative cross-sectional study was conducted among 200 PLWHA and 200 HIV uninfected adults aged 18 years and above attending an HIV clinic of CHUK. An oral examination was performed by a calibrated examiner. Dental caries were assessed using the WHO Decayed (D), Missing (M), and Filled Teeth (F) index (DMFT). Descriptive statistics, Chi-square, t-tests, and multiple binary logistic regression were used to analyze data.

Results

Overall, a higher prevalence (50.5%) of PLWHA had experienced dental caries (DMFT>0) compared to HIV uninfected counterparts (40.5%) (p = 0.045). The prevalence of Decayed teeth (D) was also higher (23.5%) among PLWHA compared to HIV uninfected persons (13.6%) (p = 0.011). The Mean(SD) DMFT scores among PLWHA and HIV uninfected participants were 2.28 (3.68) and 1.29 (2.21) respectively (p = 0.001). After performing multiple binary logistic regression analysis, the predictors of dental caries in PLWHA were being female (OR = 2.33; 95%CI = 1.14–4.75), frequent dental visits (OR = 4.50; 95% CI = 1.46–13.86) and detectable RNA viral load (OR = 4.50; 95% CI = 1.46–13.86). In HIV uninfected participants, the middle age range (36–45 years), and frequent dental visits were significantly associated with dental caries (OR = 6.61; 95%CI = 2.14–20.37) and (OR = 3.42; 95%CI: 1.337–8.760) respectively.

Conclusion

The prevalence of dental caries was higher in PLWHA than in HIV uninfected counterparts. The reported higher prevalence of caries in PLWHA was associated with being female, detectable viral load, and frequent dental visits. Therefore, there is a need for effective oral health interventions specific to PLWHA in Rwanda to raise awareness of the risk of dental caries and provide preventive oral health services among this population. To ensure timely oral health care amongPLWHA, there is a need for an effort from policymakers and other stakeholders to integrate oral health care services within the HIV treatment program in Rwanda.
Sensitivity of outcome instruments in a priori selected patient groups after traumatic brain injury: Results from the CENTER-TBI study
Authors: von Steinbuechel Nicole , Rauen Katrin , Covic Amra , Krenz Ugne , Bockhop Fabian , Mueller Isabelle , Cunitz Katrin , Polinder Suzanne , Steyerberg Ewout W. , Vester Johannes , Zeldovich Marina ,   the CENTER-TBI participants investigators
Published: April 2023

Traumatic brain injury (TBI) can negatively impact patients’ lives on many dimensions. Multiple instruments are available for evaluating TBI outcomes, but it is still unclear which instruments are the most sensitive for that purpose. This study examines the sensitivity of nine outcome instruments in terms of their ability to discriminate within and between specific patient groups, selected a priori as identified from the literature, at three different time points within a year after TBI (i.e., 3, 6, and 12 months post injury). The sensitivity of the instruments to sociodemographic (sex, age, education), premorbid (psychological health status), and injury-related (clinical care pathways, TBI and extracranial injury severity) factors was assessed by means of cross-sectional multivariate Wei-Lachin analyses. The Glasgow Outcome Scale Extended (GOSE)–the standard in the field of TBI for measuring functional recovery–demonstrated the highest sensitivity in most group comparisons. However, as single functional scale, it may not be able to reflect the multidimensional nature of the outcome. Therefore, the GOSE was used as a reference for further sensitivity analyses on more specific outcome scales, addressing further potential deficits following TBI. The physical component summary score (PCS) of the generic health-related quality of life (HRQOL) instruments (SF-36v2/-12v2) and the TBI-specific HRQOL instruments (QOLIBRI/-OS) were most sensitive in distinguishing recovery after TBI across all time points and patient groups, followed by the RPQ assessing post-concussion symptoms and the PHQ-9 measuring depression. The SF-36v2/-12v2 mental component summary score and the GAD-7 measuring anxiety were less sensitive in several group comparisons. The assessment of the functional recovery status combined with generic HRQOL (the PCS of the SF-12v2), disease-specific HRQOL (QOLIBRI-OS), and post-concussion symptoms (RPQ) can provide a sensitive, comprehensive, yet time-efficient evaluation of the health status of individuals after TBI in different patient groups.
SGHRP: Secure Greedy Highway Routing Protocol with authentication and increased privacy in vehicular ad hoc networks
Authors: Khezri Edris , Zeinali Esmaeil , Sargolzaey Hadi
Published: April 2023

VANETs are networks of connected intelligent vehicles that can communicate with each other, as well as with infrastructure and fixed roadside equipment. As a result of the lack of fixed infrastructure and open-access environment, security is crucial when sending packets. Secure routing protocols have been proposed for VANETs, but most are focused on authenticating nodes and creating a secure route, without considering confidentiality after the route is created. Using a chain of source keys validated by a one-way function, we have proposed a secure routing protocol called Secure Greedy Highway Routing Protocol (GHRP), which provides increased confidentiality over other protocols. As part of the proposed protocol, the source, destination, and intermediate nodes are authenticated using a hashing chain in the first stage, and in the second stage, one-way hashing has been used to increase data security. In order to resist routing attacks such as black hole attacks, the proposed protocol is based on the GHRP routing protocol. The proposed protocol is simulated using the NS2 simulator, and its performance is compared with that of the SAODV protocol. Based on the simulation results, the proposed protocol performs better than the mentioned protocol in terms of packet delivery rate, overhead, and average end-to-end delay.
What do we learn when we adapt to reading regional constructions?
Authors: Boland Julie E. , Atkinson Emily , De Los Santos Guadalupe , Queen Robin
Published: April 2023

We present four experiments investigating adaptation to a regional grammatical structure through reading exposure, using both the needs + past participle construction (e.g., The car needs washed) and the double modal construction (e.g. You might could go there). In each experiment, participants read two stories containing informal dialogue. Half of the participants were exposed to one of the regional constructions and half were not. Those readers exposed to the regional constructions adapted, gradually reading the novel constructions faster over 9 to 15 exemplars. The degree to which the exposed group learned the construction was tested in two ways. In the first two experiments, learning was measured by comparing reading times to acceptable and unacceptable variants of the novel constructions. Readers did not learn either the verb tense rule for the needs construction (Experiment 1) or a simple ordering rule for double modal constructions (Experiment 2). Similarly, in Experiments 3 and 4, metalinguistic judgments used to test learning revealed that participants had failed to acquire the regional grammar of either novel construction. These experiments suggest that the adaptation effects reflect learning some general properties of the experimental stimuli, not learning the syntactic constructions themselves.
Blind quality assessment of multi-exposure fused images considering the detail, structure and color characteristics
Authors: Li Lijun , Zhong Caiming , He Zhouyan
Published: April 2023

In the process of multi-exposure image fusion (MEF), the appearance of various distortions will inevitably cause the deterioration of visual quality. It is essential to predict the visual quality of MEF images. In this work, a novel blind image quality assessment (IQA) method is proposed for MEF images considering the detail, structure, and color characteristics. Specifically, to better perceive the detail and structure distortion, based on the joint bilateral filtering, the MEF image is decomposed into two layers (i.e., the energy layer and the structure layer). Obviously, this is a symmetric process that the two decomposition results can independently and almost completely describe the information of MEF images. As the former layer contains rich intensity information and the latter captures some image structures, some energy-related and structure-related features are extracted from these two layers to perceive the detail and structure distortion phenomena. Besides, some color-related features are also obtained to present the color degradation which are combined with the above energy-related and structure-related features for quality regression. Experimental results on the public MEF image database demonstrate that the proposed method achieves higher performance than the state-of-the-art quality assessment ones.
When crisis hits: Bike-Sharing platforms amid the Covid-19 pandemic
Authors: Basak Ecem , Al Balawi Ramah , Fatemi Sorouralsadat , Tafti Ali
Published: April 2023

In this work, we examine the changes in demand for bike-sharing platforms with the onset of the Covid-19 pandemic. Using the fixed-effects regression formulation of difference-in-differences, we evaluate how the demand for bike-sharing platforms changed after the first cases of Covid were discovered and after the first executive orders were implemented. Accounting for weather conditions, socio-economic characteristics, time trends, and fixed effects across cities, our findings indicate that there is an increase in daily bike-sharing trips by 22% on average after the first Covid-19 case diagnosis, and a decrease of 30% after the first executive order implementation in each municipality, using the data up to August 2020. Moreover, we observe a 22% increase in weekday-specific trip frequency after the first Covid-19 case diagnosis and a 28% decrease in weekend-specific trip frequency after the first executive order implementation. Finally, we find that there is an increase in the frequency of trips on bike-sharing platforms in more bike-friendly, transit-friendly, and pedestrian-friendly cities upon both the first Covid-19 case diagnosis and the first executive order implementation.
NAT2 global landscape: Genetic diversity and acetylation statuses from a systematic review
Authors: Gutiérrez-Virgen Jorge E. , Piña-Pozas Maricela , Hernández-Tobías Esther A. , Taja-Chayeb Lucia , López-González Ma. de Lourdes , Meraz-Ríos Marco A. , Gómez Rocío
Published: April 2023

ArylamineN-acetyltransferase 2 has been related to drug side effects and cancer susceptibility; its protein structure and acetylation capacity results from the polymorphism’s arrays on theNAT2 gene. Absorption, distribution, metabolism, and excretion, cornerstones of the pharmacological effects, have shown diversity patterns across populations, ethnic groups, and even interethnic variation. Although the 1000 Genomes Project database has portrayed the global diversity of theNAT2 polymorphisms, several populations and ethnicities remain underrepresented, limiting the comprehensive picture of its variation. TheNAT2 clinical entails require a detailed landscape of its striking diversity. This systematic review spans the genetic and acetylation patterns from 164 articles from October 1992 to October 2020. Descriptive studies and controls from observational studies expanded theNAT2 diversity landscape. Our study included 243 different populations and 101 ethnic minorities, and, for the first time, we presented the global patterns in the Middle Eastern populations. Europeans, including its derived populations, and East Asians have been the most studied genetic backgrounds. Contrary to the popular perception, Africans, Latinos and Native Americans have been significantly represented in recent years.NAT2*4, *5B, and *6A were the most frequent haplotypes globally. Nonetheless, the distribution of *5B and *7B were less and more frequent in Asians, respectively. Regarding the acetylator status, East Asians and Native Americans harboured the highest frequencies of the fast phenotype, followed by South Europeans. Central Asia, the Middle East, and West European populations were the major carriers of the slow acetylator status. The detailed panorama presented herein, expands the knowledge about the diversity patterns to genetic and acetylation levels. These data could help clarify the controversial findings between acetylator states and the susceptibility to diseases and reinforce the utility ofNAT2 in precision medicine.
Assessment of 18F-DCFPyL PSMA PET/CT and PET/MR quantitative parameters for reference standard organs: Inter-reader, inter-modality, and inter-patient variability
Authors: Lawrence Edward M. , Kieler Minnie , Cooley Greg , Wells Shane A. , Cho Steve Y.
Published: April 2023

Prostate specific membrane antigen (PSMA)-based radiotracers have shown promise for prostate cancer assessment. Evaluation of quantitative variability and establishment of reference standards are important for optimal clinical and research utility. This work evaluates the variability of PSMA-based [18F]DCFPyL (PyL) PET quantitative reference standards. Consecutive eligible patients with biochemically recurrent prostate cancer were recruited for study participation from August 2016-October 2017. After PyL tracer injection, whole body PET/CT (wbPET/CT) was obtained with subsequent whole body PET/MR (wbPET/MR). Two readers independently created regions of interest (ROIs) including a 40% standardized uptake value (SUV) threshold ROI of the whole right parotid gland and separate spherical ROIs in the superior, mid, and inferior gland. Additional liver (right lobe) and blood pool spherical ROIs were defined. Bland-Altman analysis, including limits of agreement (LOA), as well as interquartile range (IQR) and coefficient of variance (CoV) was used. Twelve patients with prostate cancer were recruited (mean age, 61.8 yrs; range 54–72 years). One patient did not have wbPET/MR and was excluded. There was minimal inter-reader SUVmean variability (bias±LOA) for blood pool (-0.13±0.42; 0.01±0.41), liver (-0.55±0.82; -0.22±1.3), or whole parotid gland (-0.05±0.31; 0.08±0.24) for wbPET/CT and wbPET/MR, respectively. Greater inter-reader variability for the 1-cm parotid gland ROIs was present, for both wbPET/CT and wbPET/MR. Comparing wbPET/CT to the subsequently acquired wbPET/MR, blood pool had a slight decrease in SUVmean. The liver as well as parotid gland showed a slight increase in activity although the absolute bias only ranged from 0.45–1.28. The magnitude of inter-subject variability was higher for the parotid gland regardless of modality or reader. In conclusion, liver, blood pool, and whole parotid gland quantitation show promise as reliable reference normal organs for clinical/research PET applications. Variability with 1-cm parotid ROIs may limit its use.
The creative interplay between hand gestures, convergent thinking, and mental imagery
Authors: Hyusein Gyulten , Göksun Tilbe
Published: April 2023

Using hand gestures benefits children’s divergent thinking and enhances verbal improvisation in adults. In the present study, we asked whether gestures were also associated with convergent thinking by activating individuals’ verbal lexicon and maintaining their visuospatial imagery. We tested young adults on verbal and visual convergent thinking, controlling for their mental imagery skills. Results showed that gestures and mental imagery skills play a role in verbal but not visual convergent thinking. Regardless of whether gestures were spontaneous or encouraged, we found a negative association between overall gesture frequency and verbal convergent thinking or individuals with low mental imagery, and a positive association for individuals with high mental imagery. Representational gestures benefited verbal convergent thinking for everyone except those who had low mental imagery and no experience with the task. Performing beat gestures hampered verbal convergent thinking in people with lower mental imagery capacity and helped those who had high mental imagery and previous experience with the task. We also found that gesturing can benefit people with lower verbal abilities on verbal convergent thinking, however, high spatial imagery abilities were required for gestures to boost verbal convergent thinking. The current study adds a new dimension to both the embodied creativity literature and the kaleidoscope of individual differences in gesture research.
Issues with RNF43 antibodies to reliably detect intracellular location
Authors: Li Shanshan , Zhang Ruyi , Lavrijsen Marla , van den Bosch Thierry P. P. , Peppelenbosch Maikel P. , Smits Ron
Published: April 2023

RNF43 is an important negative regulator of β-catenin signaling by removing Wnt-receptors from the membrane. It is often mutated in cancers, leading to aberrant Wnt-dependent nuclear translocation of β-catenin. RNF43 has also been suggested to regulate β-catenin signaling directly within the nucleus, among other proposed nuclear functions. Given the importance of RNF43 in regulating Wnt/β-catenin signaling and its potential therapeutic relevance, a proper understanding of RNF43 biology is required. However, the presumed nuclear location is mainly based on available antibodies. These same antibodies have also been used extensively for immunoblotting or immunohistochemical purposes. However, a proper evaluation of their quality to reliably detect endogenous RNF43 has not been performed. Here, using genome editing we have generated a cell line that entirely misses RNF43 exons 8 and 9, encoding the epitopes of commonly used RNF43 antibodies. Using this clone in addition to various other cell line tools, we show that four RNF43 antibodies only yield non-specific signals when applied in immunoblotting, immunofluorescence and immunohistochemical experiments. In other words, they cannot reliably detect endogenous RNF43. Our results suggest that the nuclear staining patterns are an antibody artifact and that RNF43 is unlikely to localize within the nucleus. More generally, reports using RNF43 antibodies should be interpreted with caution, at least for the RNF43 protein aspects described in these papers.
Efficient optimization techniques for resource allocation in UAVs mission framework
Authors: Razzaq Sohail , Xydeas Costas , Mahmood Anzar , Ahmed Saeed , Ratyal Naeem Iqbal , Iqbal Jamshed
Published: April 2023

This paper considers the generic problem of a central authority selecting an appropriate subset of operators in order to perform a process (i.e. mission or task) in an optimized manner. The subset is selected from a given and usually large set of ‘n’ candidate operators, with each operator having a certain resource availability and capability. This general mission performance optimization problem is considered in terms of Unmanned Aerial Vehicles (UAVs) acting as firefighting operators in a fire extinguishing mission and from a deterministic and a stochastic algorithmic point of view. Thus the applicability and performance of certain computationally efficient stochastic multistage optimization schemes is examined and compared to that produced by corresponding deterministic schemes. The simulation results show acceptable accuracy as well as useful computational efficiency of the proposed schemes when applied to the time critical resource allocation optimization problem. Distinguishing features of this work include development of a comprehensive UAV firefighting mission framework, development of deterministic as well as stochastic resource allocation optimization techniques for the mission and development of time-efficient search schemes. The work presented here is also useful for other UAV applications such as health care, surveillance and security operations as well as for other areas involving resource allocation such as wireless communications and smart grid.
Investigating changes in mental health services utilisation in England and their impact on health outcomes and wellbeing during the COVID-19 pandemic: Protocol for a health data-linkage study
Authors: Yu Ge , Vale Luke , McMeekin Peter , Ramsay Sheena E. , Fu Yu
Published: April 2023

Introduction

Linking routinely collected health care system data records for the same individual across different services and over time has enormous potential for the NHS and its patients. The aims of this data linkage study are to quantify the changes to mental health services utilisation in responses to the COVID-19 pandemic and determine whether these changes were associated with health-related outcomes and wellbeing among people living in the most deprived communities in North East and North Cumbria, England.

Methods and analysis

We will assemble a retrospective cohort of people having referred or self-referred to NHS-funded mental health services or Improving Access to Psychological Therapies (IAPT) services between 23rdMarch 2019 and 22ndMarch 2020 in the most deprived areas in England. We will link together data from retrospective routinely collected healthcare data including local general practitioner (GP) practice data, Hospital Episode Statistics admitted patient care outpatients, and A&E, Community Services Data Set, Mental Health Services Data Set, and Improving Access to Psychological Therapies Data Set. We will use these linked patient-level data to 1) describe the characteristics of the cohort prior to the lockdown; 2) investigate changes to mental health services utilised between multiple time periods of the COVID-19 lockdown including out of lockdown; 3) explore the relationship between these changes and health outcomes/wellbeing and factors that confound and mediate this relationship among this cohort.

Strengths and limitations of this study

This study comprises a deprived population-based cohort of people having referred or self-referred to NHS-funded secondary mental health services or Improving Access to Psychological Therapies (IAPT) services over an extended period of the lockdown in England (2019–2022).

This study will utilise a new longitudinal data resource that will link together detailed data from a cohort of individual participants and retrospective administrative data relating to the use of primary, secondary, and community care services.

The study period covers pre-lockdown, different lockdown and post-lockdown, and out of lockdown periods up to March 2022.

Routinely collected administrative data contain limited contextual information and represent an underestimate of total health outcomes for these individuals.

Routinely collected datasets can often been incomplete or contain missing data, which can make it difficult to accurately analyse the data and draw meaningful conclusions.

Intervention and treatment for mental health conditions are not wholly captured across these data sources and may impact health outcomes.
Level of shared decision making and associated factors among patients with mental illness in Northwest Ethiopia: Explanatory sequential mixed method study
Authors: Abate Agmas Wassie , Desalegn Wondimnew , Teshome Assefa Agegnehu , Chekol Aklile Tsega , Aschale Mastewal
Published: April 2023

Background

Shared decision-making is a patient–centered and a recovery-oriented mental health system in which consumers are encouraged to actively engage in illness management. Although shared decision-making research in mental health has evolved rapidly in the past two decades, there is a lack of studies examining the level and factors associated with shared decision-making practice in low-income countries like Ethiopia.

Methods

An institutional-based explanatory sequential mixed method study design was conducted from July 18 to September 18, 2022, at Bahir Dar city specialized hospitals. A systematic random sampling technique was used. The level of shared-decision making was measured by 9-item shared decision-making questionnaire among 423 patients with mental illness. Epicollect5 was used to collect data, which was then exported to the Statistical Package for social science version 25 for analysis. Variables with a P-value < 0.25 were considered candidates for the multivariate logistic regression analysis. The odds ratio with a 95% confidence interval was used to show the strength of the association. An in-depth interview was conducted among ten purposively selected participants.

Result

Low shared decision-making practice was found to be 49.2% (95% CI 45.9%-55.7%). The Multivariate analysis showed that low perceived compassionate care (AOR = 4.45; 95%CI 2.52–7.89), low social support (AOR = 1.72; 95% CI 1.06–2.80), and no community-based health insurance (AOR = 1.96; 95%CI l.04–3.69) were positively associated with low shared decision making. The qualitative result showed that the most common barriers to shared decision-making were a lack of empathy and a shortage of mental health workers.

Conclusion and recommendation

Almost half of the patients had low shared decision-making practices. This implies that shared decision-making requires high attention as it is essential for patient-centered care.
Maternal transfer of IgA and IgG SARS-CoV-2 specific antibodies transplacentally and via breast milk feeding
Authors: Sajadi Mohammad M. , Shokatpour Narjes , Purcell Madeleine , Tehrani Zahra Rikhtegaran , Lankford Allison , Bathula Allison , Campbell James D. , Hammershaimb Elizabeth Adrianne , Deatrick Kristopher B. , Bor Casey , Parsell Dawn M. , Dugan Colleen , Levine Andrea R. , Ramelli Sabrina C. , Chertow Daniel S. , Herr Daniel L. , Saharia Kapil K. , Lewis George K. , Grazioli Alison
Published: April 2023

Background

Although there have been many studies on antibody responses to SARS-CoV-2 in breast milk, very few have looked at the fate of these in the infant, and whether they are delivered to immunologically relevant sites in infants.

Methods

Mother/infant pairs (mothers who breast milk fed and who were SARS-CoV-2 vaccinated before or after delivery) were recruited for this cross-sectional study. Mother blood, mother breast milk, infant blood, infant nasal specimen, and infant stool was tested for IgA and IgG antibodies against SARS-CoV-2 spike trimer.

Results

Thirty-one mother/infant pairs were recruited. Breast milk fed infants acquired systemic anti-spike IgG antibodies only if their mothers were vaccinated antepartum (100% Antepartum; 0% Postpartum; P<0.0001). Breast milk fed infants acquired mucosal anti-spike IgG antibodies (in the nose) only if their mothers were vaccinated antepartum (89% Antepartum; 0% Postpartum; P<0.0001). None of the infants in either group had anti-spike IgA in the blood. Surprisingly, 33% of the infants whose mothers were vaccinated antepartum had high titer anti-spike IgA in the nose (33% Antepartum; 0% Postpartum; P = 0.03). Half-life of maternally transferred plasma IgG antibodies in the Antepartum infant cohort was ~70 days.

Conclusion

Vaccination antepartum followed by breast milk feeding appears to be the best way to provide systemic and local anti-SARS-CoV-2 antibodies for infants. The presence of high titer SARS-CoV-2-specific IgA in the nose of infants points to the potential importance of breast milk feeding early in life for maternal transfer of mucosal IgA antibodies. Expectant mothers should consider becoming vaccinated antepartum and consider breast milk feeding for optimal transfer of systemic and mucosal antibodies to their infants.
Estimating the undetected emergence of COVID-19 in the US
Authors: Javan Emily M. , Fox Spencer J. , Meyers Lauren Ancel
Published: April 2023

As SARS-CoV-2 emerged as a global threat in early 2020, China enacted rapid and strict lockdown orders to prevent introductions and suppress transmission. In contrast, the United States federal government did not enact national orders. State and local authorities were left to make rapid decisions based on limited case data and scientific information to protect their communities. To support local decision making in early 2020, we developed a model for estimating the probability of an undetected COVID-19 epidemic (epidemic risk) in each US county based on the epidemiological characteristics of the virus and the number of confirmed and suspected cases. As a retrospective analysis we included county-specific reproduction numbers and found that counties with only a single reported case by March 16, 2020 had a mean epidemic risk of 71% (95% CI: 52–83%), implying COVID-19 was already spreading widely by the first detected case. By that date, 15% of US counties covering 63% of the population had reported at least one case and had epidemic risk greater than 50%. We find that a 10% increase in model estimated epidemic risk for March 16 yields a 0.53 (95% CI: 0.49–0.58) increase in the log odds that the county reported at least two additional cases in the following week. The original epidemic risk estimates made on March 16, 2020 that assumed all counties had an effective reproduction number of 3.0 are highly correlated with our retrospective estimates (r = 0.99; p<0.001) but are less predictive of subsequent case increases (AIC difference of 93.3 and 100% weight in favor of the retrospective risk estimates). Given the low rates of testing and reporting early in the pandemic, taking action upon the detection of just one or a few cases may be prudent.
Characterizing subgroups of sexual behaviors among men who have sex with men eligible for, but not using, PrEP in the Netherlands
Authors: de la Court Feline , van Wees Daphne , van Benthem Birgit , Hoornenborg Elske , Prins Maria , Boyd Anders
Published: April 2023

This study identified subgroups of sexual behaviors associated with increased STI/HIV risk among those eligible for but not using pre-exposure prophylaxis (PrEP) in order to improve PrEP uptake and prioritization in the context of restricted capacity. We used data from sexual health centers (SHCs) in the Netherlands, including all visits of eligible but non-PrEP using men who have sex with men (MSM), men who have sex with men and women (MSMW) and transgender persons between July 2019 (start of the Dutch national PrEP pilot (NPP)) and June 2021. Using latent class analysis (LCA), we identified classes of sexual behaviors (number of partners, chemsex, group sex and sex work) and explored whether these classes were associated with STI diagnosis and sociodemographics. Across 45,582 visits of 14,588 eligible non-PrEP using individuals, the best fitting LCA model contained three classes of sexual behaviors. Classes were distinguished by seldomly reported sexual behaviors (class 1; 53.5%, n = 24,383), the highest proportions of ≥6 partners and group sex (class 2; 29.8%, n = 13,596), and the highest proportions of chemsex and sex work (class 3; 16.7% of visits, n = 7,603). Visits in classes 2 and 3 (vs. class 1) were significantly more often with individuals who were diagnosed with an STI, older (≥36 vs. ≤35 years), MSMW (vs. MSM), and visiting an urban (vs. non-urban) SHC; while these visits were significantly less often with individuals from an STI/HIV endemic area. The percentage of visits at which an STI was diagnosed was 17.07% (n = 4,163) in class 1, 19.53% (n = 2,655) in class 2 and 25.25% (n = 1,920) in class 3. The highest risk of STI, and thereby HIV, was in those engaging in specific subgroups of sexual behavior characterized by frequently reporting multiple partners, group sex, sex work or chemsex. PrEP uptake should be encouraged and prioritized for these individuals.
Is there no beauty in sexually dimorphic eyes? Facial attractiveness and White Europeans ocular morphology—Brief communication
Authors: Danel Dariusz P. , Perea-Garcia Juan Olvido , Lewandowski Zdzisław , Szala Anna , Fedurek Piotr , Kleisner Karel , Wacewicz Sławomir
Published: April 2023

The link between human ocular morphology and attractiveness, especially in the context of its potential adaptive function, is an underexplored area of research. In our study, we examined the association between facial attractiveness and three sexually dimorphic measures of ocular morphology in White Europeans: the sclera size index, width-to-height ratio, and relative iris luminance. Sixty participants (30 women) assessed the attractiveness of the opposite-sex photographs of 50 men and 50 women. Our results show that in both men and women, none of the three measures was linked to the opposite sex ratings of facial attractiveness. We conclude that those ocular morphology measures may play a limited role in human mate preferences.
Clinical practice of childhood apraxia of speech in Hong Kong: A web-based survey study
Authors: Wong Eddy C. H. , Wong Min N. , Velleman Shelley L.
Published: April 2023

Background

A survey study on clinical practice not only provides insight into the implementation of knowledge, but also informs future investigations. There is a limited understanding of childhood apraxia of speech (CAS) in Cantonese speakers. This study examined the clinical practice of CAS in Hong Kong and discussed future directions of research for better evidence-based practice.

Methods

Qualified Hong Kong pediatric speech-language pathologists (SLPs) completed the online questionnaire, which had a total of 48 questions regarding their knowledge of and experience with CAS in Cantonese speakers, including assessment, diagnosis, and treatment.

Results

Seventy-seven responses were received from Hong Kong SLPs. Most of the SLPs (83.2%) rated their understanding of CAS as either “a little” or “fair”. About half (53.2%) of the respondents had worked with children with CAS. No standardized assessment or objective/quantitative measures were used clinically. Instead, seven assessment tasks, including imitation of polysyllabic words and speech and language samples were used commonly. Perceptual judgment of clinical features is still the most popular approach for diagnosis, with a variety of lists in use. Of concern was that, in addition to using some evidence-based approaches, local SLPs treated CAS using approaches that have limited evidence, in the context of less treatment frequency, targeting both speech and language skills within the same session, and with only partial implementation of the approaches.

Conclusions

The results suggest that the understanding of CAS among local SLPs requires attention. One reason for this is that evidence regarding the assessment, diagnosis, and treatment of Cantonese speakers with CAS is still limited. Future investigations are needed.
Coupling analysis of heart rate variability and cortical arousal using a deep learning algorithm
Authors: Huo Jiayan , Quan Stuart F. , Roveda Janet , Li Ao
Published: April 2023

Frequent cortical arousal is associated with cardiovascular dysfunction among people with sleep-disordered breathing. Changes in heart rate variability (HRV) can represent pathological conditions associated with autonomic nervous system dysfunction. Previous studies showed changes in cardiac activity due to cortical arousals. However, few studies have examined the instantaneous association between cortical arousal and HRV in an ethnically diverse population. In this study, we included 1,069 subjects’ full night ECG signals from unattended polysomnography in the Multi-Ethnic Study of Atherosclerosis dataset. An automated deep learning tool was employed to annotate arousal events from ECG signals. The etiology (e.g., respiratory, or spontaneous) of each arousal event was classified through a temporal analysis. Time domain HRVs and mean heart rate were calculated on pre-, intra-, and post-arousal segments of a 25-s period for each arousal event. We observed that heart rate and HRVs increased during the arousal onsets in the intra-arousal segments, regardless of arousal etiology. Furthermore, HRVs response to cortical arousal occurrence differed according to gender and the sleep stages in which arousal occurred. The more intense HRVs variation due to arousal in females can contribute to a potentially stronger association between arousal burden and long-term mortality. The excessive abrupt sympathetic tone elevation in REM caused by arousal may provide insights on the association between sleep and sudden cardiac death.
Survey on the association between Toxoplasma gondii infection and violent behavior in inmates
Authors: Rocha-Salais Adriana , Muñoz-Larreta Fátima Yazmin , García-Pérez Sergio Ignacio , Serrato-Enríquez Alejandro Israel , Rivas-González Manuel Arturo , Sifuentes-Alvarez Antonio , Rábago-Sánchez Elizabeth , Beristain-García Isabel , Alvarado-Esquivel Cosme
Published: April 2023

We evaluated the association betweenT.gondiiseropositivity and violent behavior in a sample of inmates in Durango, Mexico. Through a cross-sectional study design, we studied 128 inmates (mean age: 35.89 ± 10.51; range: 19–65 years). Sera of participants were analyzed for anti-T.gondiiIgG antibodies using a commercially available enzyme-linked immunosorbent assay. Violence was assessed by 1) the Historical, Clinical and Risk Management-20 (HCR-20) tool; 2) the type of the crime for which inmates were convicted; and 3) the Buss-Perry Aggression Questionnaire (AGQ). Of the 128 inmates, 17 (13.3%) had high risk of violence by the HCR-20 criteria, 72 (56.3%) were considered violent by the type of the crime committed, and 59 (46.1%) were considered violent by the AGQ. Depending on the evaluation method of violence, the seroprevalence ofT.gondiiinfection in violent inmates varied from 0% to 6.9%. No statistically significant difference in anti-T.gondiiIgG seroprevalence between violent and non-violent inmates was found (for instance by AGQ, OR: 1.17; 95% CI: 0.22–6.07;P= 1.00). Mean scores of the AGQ inT.gondiiseropositive inmates (73.67 ± 29.09; 95% CI: 50.00–99.31) were similar to those (79.84 ± 25.00; 95% CI: 75.46–84.27) found inT.gondiiseronegative inmates (P= 0.55). Mean scores of anger, psychical aggression, verbal aggression, and hostility inT.gondiiseropositive inmates were similar to those found inT.gondiiseronegative inmates. Results of this study suggest that infection withT.gondiiis not associated with violence in inmates in Durango, Mexico. Further studies with larger sample sizes and in several correctional facilities to determine the association betweenT.gondiiinfection and violence in inmates are needed.
Cell death as a defense strategy against pathogens in plants and animals
Authors: Salguero-Linares Jose , Coll Nuria S.
Published: April 2023

Eukaryotes are endowed with sophisticated innate immune systems to recognize non-self and halt pathogen proliferation. Activation of cell death at the site of attempted pathogen ingress is a common strategy used by plants and animals to restrict pathogen proliferation and trigger immune responses in the surrounding tissues. As such, immunogenic cell death shares several features in both plants and animals that will be discussed in this article, namely: (i) it is triggered by activation of NLR immune receptors—often through oligomerization; (ii) it results in disruption of the plasma membrane (PM)/endomembrane integrity driving an imbalance in ion fluxes; and (iii) it results in the release of signaling molecules from dying cells.