bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Hemodynamic Effects of Intravenous Amiodarone Formulations During the Maintenance Phase Infusion
Authors: Lindquist Desirae E. , Rowe A. Shaun , Heidel Eric , Fleming Travis , Yates John R.
Published: September 2015

Background: Two of the excipients in intravenous formulations of amiodarone, polysorbate 80 and benzyl alcohol, have been shown to cause hypotension. A newer formulation of amiodarone, which contains cyclodextrin, is devoid of these excipients. Objective: To evaluate the change in mean arterial pressure when utilizing 2 intravenous amiodarone formulations. Methods: This was a retrospective cohort analysis conducted at an academic medical center. Patients received intravenous amiodarone containing either polysorbate 80/benzyl alcohol (control) or cyclodextrin (cyclodextrin). Patients received these formulations based on a standard institutional protocol of 1 mg/min for 6 hours, followed by 0.5 mg/min for at least 18 hours or until discontinued by the provider. All data were collected from the medical record and included changes in blood pressures, time to lowest systolic blood pressure, concurrent antihypertensive use, and number of patients requiring treatment for hypotension. Results: A total of 160 patients (120 control, 40 cyclodextrin) were included. There was a statistically significant difference in mean arterial pressure between the groups receiving the control formulation of amiodarone compared with the cyclodextrin formulation across the 24-hour maintenance phase infusion ( P < 0.001). There was a significant difference between formulations with regard to the change in mean arterial pressure during the 0- to 6-hour and 12- to 18-hour time blocks. There was a statistically significant difference in the number of patients receiving fluid boluses for treatment of hypotension ( P = 0.001). Conclusions: The excipients in the formulation of intravenous amiodarone may have a significant role in the hypotensive effects seen throughout the duration the maintenance phase infusion.
Evaluation of Statin Prescribing for Secondary Prevention in Primary Care Following New Guideline Recommendations
Authors: Zupec Jason F. , Marrs Joel C. , Saseen Joseph J.
Published: September 2015

Background: The American College of Cardiology/American Heart Association (ACC/AHA) Guideline on the Treatment of Blood Cholesterol recommends high-intensity statin therapy for most patients with established atherosclerotic cardiovascular disease (ASCVD) versus previously recommended low-density lipoprotein cholesterol targets. The impact of the ACC/AHA guidelines on prescribing patterns in primary care is uncertain. Objective: To describe the prescribing habits of statin therapy in primary care patients with ASCVD before and after the ACC/AHA guidelines were published. Methods: This retrospective observational study evaluated patients with ASCVD who were seen in at least 1 of 8 primary care clinics in the University of Colorado Health system. It received expedited approval by the Colorado Multiple Institutional Review Board. The primary outcome measure was the proportion of patients with established ASCVD prescribed high-intensity statin therapy within 1 year before or after guideline release. Results: In total, 220 patients were included in the analysis with 110 in the before and 110 in the after cohort. For the primary outcome analysis, the rate of high-intensity statin utilization in the before versus after groups was significantly greater (25.5% vs 41.8%, P = 0.01). For ages 76 to 89 years, 36 of 37 and 29 of 30 patients in the before and after groups were receiving moderate- to high-intensity statin therapy (97.3% vs 96.7%, P = 0.99). Subgroup analysis in the after cohort for all ages showed no change in statin therapy for 77% of patients. Conclusions: High-intensity statin prescribing increased in patients with ASCVD after release of the ACC/AHA cholesterol guidelines. Our data indicate that national evidence-based guidelines may influence clinical practice in very high risk patients.
A Large State Medicaid Outpatient Advanced Imaging Utilization Management Program
Authors: Rapoport Robert J. , Parker Laurence , Levin David C. , Hiatt Mark D.
Published: September 2015

A decade of rapidly rising outpatient advanced imaging utilization ended toward the end of the past decade, with slow growth since. This has been attributed to repetitive reimbursement cuts, medical radiation exposure concerns, increasing deductibles and patient copayments, and the influence of radiology benefit management companies. State Medicaid programs have been reluctant to institute radiology benefit management preauthorization programs since the time burden for obtaining test approval could cause providers to drop out. Also, these patients may lack the knowledge to appeal denials, and medically necessary tests could be denied with adverse outcomes. Little data exist demonstrating the efficacy of such programs in decreasing utilization and cost. We report a 2-year experience with an outpatient advanced imaging prior notification program for a large state Medicaid fee-for-service population. The program did not allow any denials, but nevertheless the data reveal a large, durable decrease in advanced imaging utilization and cost.
“I Didn’t Know Whether I Was Right or Wrong or Just Bewildered”
Authors: Towns Alison J. , Adams Peter J.
Published: September 2015

Little has been written about the impact on women of the man’s obscuring and deflecting of responsibility when domestic violence is used by him against her. Women’s advocates report that women who seek shelter from domestic violence assume blame for the violence and struggle to shift from this position of responsibility. Women are likely to be silenced if they assume responsibility and are less likely to come forward. In this study, we use discourse analysis to describe the ambiguities around responsibility that worked to silence 20 women and the sociocultural influences that supported this ambiguity. We discuss the implications for prevention.
Depression and the Performance of Masculinity in a Military Retirement Community
Authors: de Medeiros Kate , Rubinstein Robert L.
Published: September 2015

Depression can be viewed as a psychiatric illness and, as a folk ideology, part of the conceptual world of everyday life for many people. In public culture, ideas about depression are often associated with culturally feminine traits (e.g., expressed sadness, uncontrollable crying, and other forms of emotionality) and in contrast to a sense of rationality publicly ascribed (however incorrectly) to men. Such gendered traits can consequently pose challenges to constructions of masculinity in older men who experience forms of stress related to loss and finitude. This article draws upon interviews from a unique sample—eight older men who reside in a military-sponsored retirement community—to explore how masculinity is performed and defended in light of age- and person-based threats such as depression. Findings point to the importance of the lifelong identity as a military serviceman as an important site through which to negotiate, process, or deny change with age.
Party organizational strength and technological capacity
Authors: Hatch Rebecca S
Published: September 2015

In their study of the state-level party organizations in the US, Cotter et al. (1984) introduce a theory of party organizational strength, arguing that the strongest parties exhibit both organizational complexity and programmatic capacity. In the present era, organizational complexity and programmatic capacity still emerge as the primary dimensions of party organizational strength, but this research demonstrates the importance of new indicators of party technological capacity to our understanding of party effectiveness in the early 21st century. In particular, parties that hire technologically-oriented staff are best equipped to carry out party programmatic activities, as they have the capacity to conduct voter mobilization programs and sophisticated data analytics in an online environment. Using data from a 2011 survey of the state party organizations in the US, this research shows that party organizational strength and party technological capacity are interrelated and overlapping concepts and are essential to understanding the parties’ programmatic and voter outreach capabilities.
Exporting criminological innovation abroad: Discursive representation, ‘evidence-based crime prevention’ and the post-neoliberal development agenda in Latin America
Authors: Blaustein Jarrett
Published: September 2015

The aim of this article is to stimulate a critical dialogue about the implications of northern criminologists working to promote their research abroad. It accounts for why attempts to generate impact on an international scale may prove problematic and illustrates potential pitfalls by analysing the content and discourses featured in a toolkit for evidence-based crime prevention developed for the Inter-American Development Bank in 2012. The example prompts important and timely questions about the practical and discursive implications of northern attempts to influence policy and practice in the South. The article concludes by accounting for the importance of reflexivity as a strategy for limiting this harm-generating potential and for fostering discursively representative policy deliberations.
Anti-Immigrant Anxieties in State Policy: The Great Recession and Punitive Immigration Policy in the American States, 2005–2012
Authors: Ybarra Vickie D. , Sanchez Lisa M. , Sanchez Gabriel R.
Published: September 2015

The Great Recession of late 2007 through 2009 had profound negative economic impacts on the U.S. states, with 49 states experiencing revenue decreases in their 2009 budgets representing more than $67.2 billion USD. Also during this period, states enacted a record number of laws related to immigrants residing in their states. We make use of data from the National Conference of State Legislatures (NCSL) to examine punitive immigration policy enactment from 2005 to 2012 and conduct a state comparative study using cross-sectional time-series analysis to examine the potential ways in which the economic recession and changing demographics in the states have impacted punitive state immigration policy making. We hypothesize that although anti-immigrant anxieties are driven in part by economic insecurity, they are also impacted by the presence of a large or growing proportion of racialized immigrants. We find that increases in state Hispanic populations and state economic stressors associated with the recession have both led to a greater number of enacted punitive state immigration policies. In addition, we find that changes in the non-Hispanic white populations in the states are also impacting the expression of anti-immigrant attitudes in state policy during this period.
Technical note: exploiting problem definition study for cyber security simulations
Authors: Cankaya Yilmaz
Published: September 2015

In this paper, the problem definition study for a cyber security simulation, which has an important impact on the quality of the expected outcome of the simulation process, is exploited. This paper identifies the obstacles and unclear points in determining the objectives, boundary, domain, and the level of detail of a cyber security model, as well as the specific issues that arise. It also introduces a method to increase the level of detail of a model on hand of an example attack-and-defense scenario.
The Effectiveness of Visual Short-Time Neurofeedback on Brain Activity and Clinical Characteristics in Alcohol Use Disorders
Authors: Lackner Nina , Unterrainer Human F. , Skliris Dimitris , Wood Guilherme , Wallner-Liebmann Sandra J. , Neuper Christa , Gruzelier John H.
Published: September 2015

The present study was carried out to examine the efficacy of alpha/theta neurofeedback (NF) with a new visual paradigm in a cohort of alcohol use disordered (AUD) patients (n = 25) treated in an Austrian therapeutic community center. The experimental study design focused on changes in absolute and relative resting EEG band power as well as in clinical variables, including depression (Beck Depresion Inventory [BDI-V]), psychiatric symptoms (Brief Symptom Inventory [BSI], coping (Freiburg Questionnaire on Coping with Illness [FKV-lis]), psychotherapy motivation (Therapy Motivation Questionnaire [FPTM-23]), sense of coherence (Sense of Coherence Scale [SOC-13]), posttraumatic growth (Posttraumatic Growth Inventory [PPR]), and alcohol cravings (Alcohol Craving Questionnaire [ACQ]). For measuring training effects, participants were randomly allocated to 2 groups: an experimental group (EG, n = 13) and a control group (CG, n = 12). Patients in EG received 12 sessions of visual NF training over a period of 6 weeks to enhance alpha (8-12 Hz) and theta (4-7 Hz) frequency band power in addition to the standard treatment program of the rehabilitation center. Participants in CG received no additional NF intervention. The multivariate analysis of covariance (MANCOVA) showed a change by trend in absolute alpha and theta power in the EG. Even though no MANCOVA effects were found in the clinical scales, AUD patients reported increasing control of their brain activity during the course of NF. However, changes in several clinical scales (BDI-V, BSI, FKV-lis, PPR) from pre- to posttest were observed only in the EG contrary to the CG. The findings of this pilot study provide first evidence for the practicality and effectiveness of visual short-term NF as an additive intervention in the therapeutic community.
A differential evolution algorithm for tooth profile optimization with respect to balancing specific sliding coefficients of involute cylindrical spur and helical gears
Authors: Abderazek Hammoudi , Ferhat Djeddou , Atanasovska Ivana , Boualem Keskes
Published: September 2015

Profile shift has an immense effect on the sliding, load capacity, and stability of involute cylindrical gears. Available standards such as ISO/DIS 6336 and BS 436 DIN/3990 currently give the recommendation for the selection of profile shift coefficients. It is, however, very approximate and usually given in the form of implicit graphs or charts. In this article, the optimal selection values of profile shift coefficients for cylindrical involute spur and helical gears are described, using a differential evolution algorithm. The optimization procedure is developed specifically for exact balancing specific sliding coefficients at extremes of contact path and account for gear design constraints. The obtained results are compared with those of standards and research of other authors. They demonstrate the effectiveness and robustness of the applied method. A substantial improvement in balancing specific sliding coefficients is found in this work.
Experimental investigation of flow boiling heat transfer and pressure drops characteristic of R1234ze(E), R600a, and a mixture of R1234ze(E)/R32 in a horizontal smooth tube
Authors: Qiu Jinyou , Zhang Hua , Yu Xiaoming , Qi Yingxia , Lou Jiangfeng , Wang Xi
Published: September 2015

The saturated flow boiling heat transfer coefficients and frictional pressure drops characteristics of R1234ze(E), R600a, and L-41b (R1234ze(E)/R32 (27/73 mass %)) inside an 8 mm inner diameter horizontal tube were investigated. The experiment was carried out at the saturation temperature of 20°C with heat flux ranging from 5.0 to 10.0 kW·m−2 and mass flux ranging from 200 to 400 kg·m−2·s−1. The influence of mass flux, heat flux, and quality on the heat transfer coefficients and frictional pressure drops were examined and discussed. The results show that the local heat transfer coefficients of R1234ze(E) are averagely 33% and 18% lower than those of R600a and L-41b, respectively. The frictional pressure drops of R1234ze(E) are 21% lower than those of R600a but 6% greater than those of L-41b. Meanwhile, the experimental data of local heat transfer coefficients and frictional pressure drops are compared with some well-known correlations available in literatures.
Countering Islamophobic media representations: The potential role of peace journalism
Authors: Anderson Leticia
Published: September 2015

Representations of Muslims in the Australian media have been routinely stereotyped; have failed to reflect the diversity of origin, outlook, and aspirations of Muslim Australians; and have negatively impacted perceptions of Islam and treatment of Muslims by non-Muslim Australians. This article explores findings from a study involving content and discourse analysis of representations of Muslims in Australian broadsheet newspapers. Lower levels of Islamophobia in news articles within the sample were associated with a ‘peace journalism’ approach to reporting. Peace journalism promotes the contextualization of conflict narratives and challenges dominant news conventions such as the focus on elite, bureaucratic sources. It is therefore suggested that potentially fruitful strategies for countering Islamophobia in the news media could include the adoption of standards for conflict reporting and expanding opportunities for peace journalism in reporting on issues relating to Muslims and Islam.
Low-dose fenfluramine in the treatment of neurologic disorders: experience in Dravet syndrome
Authors: Schoonjans An-Sofie , Lagae Lieven , Ceulemans Berten
Published: September 2015

In this paper, we review the experience with fenfluramine in epileptic and other paroxysmal disorders. Since the best available data are from the treatment of Dravet syndrome, we will focus primarily on this condition. Originally fenfluramine was launched as an anorectic agent. As early as 1985, seizure reduction in children could be demonstrated in a few cases with photosensitive, self-induced epilepsy. Hereafter, a small study was launched in patients with self-induced epilepsy. Results showed a significant seizure reduction, and review of the patient data showed that 5 of the 12 patients had Dravet syndrome. During that observation period, fenfluramine was withdrawn from the market because of cardiovascular side effects associated with prescribing higher doses in combination with phentermine for weight loss. In March 2002, a Belgian Royal Decree was issued permitting further study of fenfluramine in pediatric patients with intractable epilepsy. In 2011 under the Royal Decree, a prospective study of patients with Dravet syndrome treated with low-dose fenfluramine was initiated and is currently ongoing. The initial results are promising in terms of reduction of seizure frequency and overall tolerability.
Combined laparoscopic pyelolithotomy and endoscopic pyelolithotripsy for staghorn calculi: long-term follow-up results from a case series
Authors: Pastore Antonio Luigi , Palleschi Giovanni , Silvestri Luigi , Leto Antonino , Ripoli Andrea , Fuschi Andrea , Al Salhi Yazan , Autieri Domenico , Petrozza Vincenzo , Carbone Antonio
Published: September 2015

Purpose:

Staghorn renal stones are a challenging field in urology. Due to their high recurrence rates, particularly those associated with an infective process, a complete removal is the ultimate goal in their management. We report our experience with a combined approach of laparoscopic pyelolithotomy and endoscopic pyelolithotripsy, the stone clearance rate, and long-term, follow-up outcomes.

Methods:

From June 2012 to October 2014, nine adult patients with large staghorn renal calculi (mean size, 7.2 cm; range, 6.2–9.0 cm) underwent a combined laparoscopic and endoscopic approach. The technique comprised laparoscopic pyelolithotomy and holmium-YAG laser stone fragmentation with the use of a flexible cystoscope introduced through a 12 mm trocar.

Results:

The average operative time was 140 min (range, 90–190 min). The mean estimated hemoglobin loss was 0.6 mmol/l (range 0.5–0.7 mmol/l). None of the patients required an open- surgery conversion. The mean hospital stay was 4 days (range, 2–6 days). A computed tomography urogram control at 6 months of follow up did not show any stone recurrence.

Conclusions:

Laparoscopic pyelolithotomy combined with endoscopic pyelolithotripsy could be a therapeutic option in cases where mini-invasive procedures, that is, extracorporeal shock wave lithotripsy, ureteroscopic lithotripsy, and percutaneous nephrolithotomy (PCNL) have failed. This technique has a high stone-clearance rate (75–100%) comparable with open surgery and PCNL. However, it could be technically demanding and should be performed by skilled laparoscopy surgeons.
Diversifying Science
Authors: Woodcock Anna , Hernandez Paul R. , Schultz P. Wesley
Published: September 2015

Stereotypes influence academic interests, performance, and ultimately career goals. The long-standing National Institutes of Health Research Initiative for Scientific Enhancement (RISE) training program has been shown to be effective at retaining underrepresented minorities in science. We argue that programs such as RISE may alter the experience and impact of stereotype threat on academic achievement goals and future engagement in a scientific career. We report analyses of a national sample comparing RISE students with a propensity score-matched control group over a 6-year period. Mediation analyses revealed that while RISE program membership did not buffer students from stereotype threat, it changed students’ downstream responses and ultimately their academic outcomes. Nonprogram students were less likely than RISE students to persist in the sciences, partially because feelings of stereotype threat diminished their adoption of mastery goals. We discuss how these findings inform stereotype threat and goal orientation theories and provide insight into the success of intervention programs.
Towards ‘Engagement 2.0’: Insights from a study of dynamic consent with biobank participants
Authors: Teare Harriet JA , Morrison Michael , Whitley Edgar A , Kaye Jane
Published: September 2015

Web 2.0 technologies have enabled new methods of engagement, moving from static mono-directional sources of information to interactive user-led experiences. Use of Web 2.0 technologies for engagement is gaining momentum within the health sector however this is still in its infancy in biobanking research. This paper reports on findings from focus groups with biobank participants to gauge their views on a Web 2.0 dynamic consent interface. The findings from this study suggest that participants would welcome more interactive engagement with biobanks, and the opportunity to hear more about how their data and samples are being used in research. We propose that by adopting Web 2.0 tools for dynamic consent, we can move towards an ‘Engagement 2.0’ model whereby research participants have the opportunity for more interactive engagement with medical research, setting up a two-way communication channel between participants and researchers, for the benefit of both.
Evaluation of an integrated multidisciplinary approach in multiple sclerosis care: A prospective, randomized, controlled study
Authors: Papeix Caroline , Gambotti Laetitia , Assouad Rana , Ewenczyck Claire , Tanguy Marie-Laure , Pineau Fanny , Houis Marie-Claire , Mazevet Dominique , Maillart Elisabeth , Lubetzki Catherine
Published: September 2015

Background

Disabled multiple sclerosis (MS) patients often need intervention of multiple specialists, resulting in a complex organization of care. How this multidisciplinary care should be organized and structured has not been studied.

Objective

The objective of this article is to address the effectiveness of an integrated multidisciplinary approach versus usual care in MS patients.

Methods

This is a prospective, randomized, controlled, monocentric clinical trial in MS patients. Two treatment strategies were compared: (i) an integrated multidisciplinary (IMD) approach, consisting of a half-day individually tailored comprehensive assessment in the MS clinic; and (ii) a standard care. The primary outcome was the impact of the strategy on quality of life (QoL) measured using the MSIS-29 scale at inclusion and after six months.

Results

Fifty MS patients were included. Median MSIS 29 score decreased over six months in the control group (−4.89) and increased in the IMD group (+2.00), with a significant difference between the two groups ( p = 0.03). However, in the multivariate analysis, after adjustment of HAD-D and INTERMED score, this difference was no longer significant.

Conclusions

This prospective, randomized study is the first attempt to evaluate the multidisciplinary approach in MS patients. The results show that, contrary to our expectations, an integrated multidisciplinary approach is not superior to usual care on QoL.
Predictors and Outcomes of Burnout in Primary Care Physicians
Authors: Rabatin Joseph , Williams Eric , Baier Manwell Linda , Schwartz Mark D. , Brown Roger L. , Linzer Mark
Published: September 2015

Objective: To assess relationships between primary care work conditions, physician burnout, quality of care, and medical errors. Methods: Cross-sectional and longitudinal analyses of data from the MEMO (Minimizing Error, Maximizing Outcome) Study. Two surveys of 422 family physicians and general internists, administered 1 year apart, queried physician job satisfaction, stress and burnout, organizational culture, and intent to leave within 2 years. A chart audit of 1795 of their adult patients with diabetes and/or hypertension assessed care quality and medical errors. Key Results: Women physicians were almost twice as likely as men to report burnout (36% vs 19%, P < .001). Burned out clinicians reported less satisfaction ( P < .001), more job stress ( P < .001), more time pressure during visits ( P < .01), more chaotic work conditions ( P < .001), and less work control ( P < .001). Their workplaces were less likely to emphasize work-life balance ( P < .001) and they noted more intent to leave the practice (56% vs 21%, P < .001). There were no consistent relationships between burnout, care quality, and medical errors. Conclusions: Burnout is highly associated with adverse work conditions and a greater intention to leave the practice, but not with adverse patient outcomes. Care quality thus appears to be preserved at great personal cost to primary care physicians. Efforts focused on workplace redesign and physician self-care are warranted to sustain the primary care workforce.
The Media Representations of Police Image
Authors: Chan Wayne W. L.
Published: September 2015

The Hong Kong Police Force has undergone one of its biggest challenges in the Occupy Movement that emerged in the last year. Despite the sheer complexity of the police roles, we know little about its representations in the media coverage, and even less about the extent to which the imagery impacts of police acting as peacekeepers would have been made upon the images of police acting as crime fighters. Against this background, this empirical research aims to investigate the police image and its relation to the police’s specifically categorized duties in Hong Kong. The content analysis of local newspaper accounts is used as the research method. It is found that there would be generally negative media representations of police in the order-maintenance work whereas the police images in the crime-fighting duties could still remain positive. The reasons for these findings and their implications for the conceptions of the police role are discussed.
Mitochondrial Myopathy in Follow-up of a Patient With Chronic Fatigue Syndrome
Authors: Galán Fernando , de Lavera Isabel , Cotán David , Sánchez-Alcázar José A.
Published: September 2015

Introduction. Symptoms of mitochondrial diseases and chronic fatigue syndrome (CFS) frequently overlap and can easily be mistaken. Methods. We report the case of a patient diagnosed with CFS and during follow-up was finally diagnosed with mitochondrial myopathy by histochemical study of muscle biopsy, spectrophotometric analysis of the complexes of the mitochondrial respiratory chain, and genetic studies. Results. The results revealed 3% fiber-ragged blue and a severe deficiency of complexes I and IV and several mtDNA variants. Mother, sisters, and nephews showed similar symptoms, which strongly suggests a possible maternal inheritance. The patient and his family responded to treatment with high doses of riboflavin and thiamine with a remarkable and sustained fatigue and muscle symptoms improvement. Conclusions. This case illustrates that initial symptoms of mitochondrial disease in adults can easily be mistaken with CFS, and in these patients a regular reassessment and monitoring of symptoms is recommended to reconfirm or change the diagnosis.
Metallogeny of the North Atlantic Craton in Greenland
Authors: Kolb Jochen , Bagas Leon , Fiorentini Marco L.
Published: September 2015

The North Atlantic Craton (NAC) extends along the coasts of southern Greenland. At its northern and southern margins, Archaean rocks are overprinted by Palaeoproterozoic orogeny or overlain by younger rocks. Typical granite-greenstone and granite-gneiss complexes represent the entire Archaean, with a hiatus from ∼3.55–3.20 Ga. In the granulite- and amphibolite-facies terranes, the metallogeny comprises hypozonal orogenic gold and Ni-PGE-Cr-Ti-V in mafic-ultramafic magmatic systems. Gold occurrences are widespread around and south of the capital, Nuuk. Nickel mineralization in the Maniitsoq Ni project is hosted in the Norite belt; Cr and PGE in Qeqertarssuatsiaq, and Ti-V in Sinarsuk in the Fiskenæsset complex. The lower-grade metamorphic Isua greenstone belt hosts the >1000 Mt Isua iron deposit in an Eoarchaean banded iron formation. Major Neoarchaean shear zones host mesozonal orogenic gold mineralization over considerable strike length in South-West Greenland. The current metallogenic model of the NAC is based on low-resolution data and variable geological understanding, and prospecting has been the main exploration method. In order to generate a robust understanding of the metal endowment, it is necessary to apply an integrated and collective approach. The NAC is similar to other well-endowed Archaean terranes but is underexplored, and is therefore likely to host numerous targets for greenfields exploration.
Sm-Nd and Lu-Hf isotope and trace-element systematics of Mesoarchaean amphibolites, inner Ameralik fjord, southern West Greenland
Authors: Szilas Kristoffer , Hoffmann J. Elis , Hansmeier Christina , Hollis Julie A. , Münker Carsten , Viehmann Sebastian , Kasper Haino U.
Published: September 2015

Fragmented supracrustal rocks are typical components of Archaean high-grade gneiss terranes, such as those in the North Atlantic Craton. Here we present the first major, trace element and Nd-Hf isotope data for amphibolites collected in the yet poorly studied southern inner Ameralik fjord region of southern West Greenland. In addition, new U-Pb zircon ages were obtained from the surrounding TTG gneisses.

Based on their trace-element patterns, two different groups of amphibolites can be distinguished. Following screening for post-magmatic alteration and outlying ε values, a reduced sample set defines a147Sm/143Nd regression age of 3038 Ma ±310 Ma (MSWD = 9.2) and a176Lu/176Hf regression age of 2867 ±160 Ma (MSWD = 5.5). Initial εNd2970Mavalues of the least-altered amphibolites range from 0.0 to +5.7 and initial εHf2970Ma range from +0.7 to +10.4, indicating significant isotopic heterogeneity of their mantle sources with involvement of depleted domains as well as crustal sources.

Surprisingly, the amphibolites which are apparently most evolved and incompatible element-rich have the most depleted Hf-isotope compositions. This apparent paradox may be explained by the sampling of a local mantle source region with ancient previous melt depletion, which was re-enriched by a fluid component during subduction zone volcanism or alternatively by preferential melting of an ancient pyroxenite component in the mantle source of the enriched rocks.
Life habits, hox genes, and affinities of a 311 million-year-old holometabolan larva
Authors: Haug Joachim T. , Labandeira Conrad C. , Santiago-Blay Jorge A. , Haug Carolin , Brown Susan
Published: September 2015

Background

Holometabolous insects are the most diverse, speciose and ubiquitous group of multicellular organisms in terrestrial and freshwater ecosystems. The enormous evolutionary and ecological success of Holometabola has been attributed to their unique postembryonic life phases in which nonreproductive and wingless larvae differ significantly in morphology and life habits from their reproductive and mostly winged adults, separated by a resting stage, the pupa. Little is known of the evolutionary developmental mechanisms that produced the holometabolous larval condition and their Paleozoic origin based on fossils and phylogeny.

Results

We provide a detailed anatomic description of a 311 million-year-old specimen, the oldest known holometabolous larva, from the Mazon Creek deposits of Illinois, U.S.A. The head is ovoidal, downwardly oriented, broadly attached to the anterior thorax, and bears possible simple eyes and antennae with insertions encircled by molting sutures; other sutures are present but often indistinct. Mouthparts are generalized, consisting of five recognizable segments: a clypeo-labral complex, mandibles, possible hypopharynx, a maxilla bearing indistinct palp-like appendages, and labium. Distinctive mandibles are robust, triangular, and dicondylic. The thorax is delineated into three, nonoverlapping regions of distinctive surface texture, each with legs of seven elements, the terminal-most bearing paired claws. The abdomen has ten segments deployed in register with overlapping tergites; the penultimate segment bears a paired, cercus-like structure. The anterior eight segments bear clawless leglets more diminutive than the thoracic legs in length and cross-sectional diameter, and inserted more ventrolaterally than ventrally on the abdominal sidewall.

Conclusions

Srokalarva berthei occurred in an evolutionary developmental context likely responsible for the early macroevolutionary success of holometabolous insects. Srokalarva berthei bore head and prothoracic structures, leglet series on successive abdominal segments – in addition to comparable features on a second taxon eight million-years-younger – that indicates Hox-gene regulation of segmental and appendage patterning among earliest Holometabola. Srokalarva berthei body features suggest a caterpillar-like body plan and head structures indicating herbivory consistent with known, contemporaneous insect feeding damage on seed plants. Taxonomic resolution places Srokalarva berthei as an extinct lineage, apparently possessing features closer to neuropteroid than other holometabolous lineages.
Multilocus species trees and species delimitation in a temporal context: application to the water shrews of the genus Neomys
Authors: Igea Javier , Aymerich Pere , Bannikova Anna A. , Gosálbez Joaquim , Castresana Jose
Published: September 2015

Background

Multilocus data are becoming increasingly important in determining the phylogeny of closely related species and delimiting species. In species complexes where unequivocal fossil calibrations are not available, rigorous dating of the coalescence-based species trees requires accurate mutation rates of the loci under study but, generally, these rates are unknown. Here, we obtained lineage-specific mutation rates of these loci from a higher-level phylogeny with a reliable fossil record and investigated how different choices of mutation rates and species tree models affected the split time estimates. We implemented this strategy with a genus of water shrews,Neomys, whose taxonomy has been contentious over the last century.

Results

We sequenced 13 introns and cytochromebfrom specimens of the three species currently recognized in this genus including two subspecies ofN. anomalusthat were originally described as species. A Bayesian multilocus species delimitation method and estimation of gene flow supported that these subspecies are distinct evolutionary lineages that should be treated as distinct species:N. anomalus(sensu stricto), limited to part of the Iberian Peninsula, andN. milleri, with a larger Eurasian range. We then estimated mutation rates from a Bayesian relaxed clock analysis of the mammalian orthologues with several fossil calibrations. Next, using the estimatedNeomys-specific rates for each locus in an isolation-with-migration model, the split time for these sister taxa was dated at 0.40 Myr ago (with a 95 % confidence interval of 0.26 – 0.86 Myr), likely coinciding with one of the major glaciations of the Middle Pleistocene. We also showed that the extrapolation of non-specific rates or the use of simpler models would lead to very different split time estimates.

Conclusions

We showed that the estimation of rigorous lineage-specific mutation rates for each locus allows the inference of robust split times in a species tree framework. These times, in turn, afford a better understanding of the timeframe required to achieve isolation and, eventually, speciation in sister lineages. The application of species delimitation methods and an accurate dating strategy to the genusNeomyshelped to clarify its controversial taxonomy.
Mesenchymal stem cells stabilize the blood–brain barrier through regulation of astrocytes
Authors: Park Hyun Jung , Shin Jin Young , Kim Ha Na , Oh Se Hee , Song Sook K. , Lee Phil Hyu
Published: September 2015

Introduction

The blood–brain barrier (BBB) protects the brain against potentially neurotoxic molecules in the circulation, and loss of its integrity may contribute to disease progression in neurodegenerative conditions. Recently, the active role of reactive astrocytes in BBB disruption has become evident in the inflamed brain. In the present study, we investigated whether mesenchymal stem cell (MSC) treatment might modulate reactive astrocytes and thus stabilize BBB integrity through vascular endothelial growth factor A (VEGF-A) signaling in inflammatory conditions.

Methods

For the inflamed brain, we injected LPS using a stereotaxic apparatus and MSCs were injected into the tail vein. At 6 hours and 7 days after LPS injection, we analyzed modulatory effects of MSCs on the change of BBB permeability through VEGF-A signaling using immunochemistry and western blot. To determine the effects of MSCs on VEGF-A-related signaling in cellular system, we had used endothelial cells treated with VEGF-A and co-cultured astrocyte and BV 2 cells treated with lipopolysaccharide (LPS) and then these cells were co-cultured with MSCs.

Results

In LPS-treated rats, MSCs restored Evans blue infiltration and the number of endothelial-barrier antigen (EBA) and P-glycoprotein (p-gp)-expressing cells, which were significantly altered in LPS-treated animals. Additionally, MSC administration following LPS treatment markedly increased the density of astrocytic filaments around vessels and reversed LPS-induced elevations in VEGF-A levels as well as endothelial nitric oxide synthase (eNOS)-dependent downregulation of tight junction proteins in the endothelium. Consequently, MSC treatment reduced neutrophil infiltration and enhanced survival of midbrain dopaminergic neurons in LPS-treated animals. In cellular system, MSC treatment led to a significant reversion of VEGF-A-induced eNOS and tight junction protein expression in endothelial cells, which led to increased EBA expressing cells. Additionally, MSC treatment significantly attenuated LPS-induced increased expressions of IL-1β in microglia and VEGF-A in astrocytes with an increase in IL-10 levels.

Conclusion

The present study indicated that MSCs may stabilize BBB permeability by modulating astrocytic endfeet and VEGF-A signaling, which may be relevant to the treatment of Parkinsonian diseases as a candidate for disease modifying therapeutics.
Some fixed point theorems in Menger probabilistic metric-like spaces
Authors: Roldán López de Hierro Antonio Francisco , de la Sen Manuel
Published: September 2015

In this manuscript we extend very recent fixed point theorems in the setting of Menger spaces in three senses: on the one hand, we introduce the notion of Menger probabilistic metric-like space by avoiding a non-necessary constraint (at least, for our purposes) in the properties that define a Menger space; on the other hand, we consider a more general class of auxiliary functions in the contractivity condition; finally, we show that the function $t\rightarrow1/t-1$
t
→
1
/
t
−
1
(which appears in many fixed point theorems in the fuzzy context) can be replaced by more appropriate and general functions. We illustrate our main statements with an example in which previous results cannot be applied.
Approximation of head and neck cancer volumes in contrast enhanced CT
Authors: Dejaco D. , Url C. , Schartinger V. H. , Haug A. K. , Fischer N. , Riedl D. , Posch A. , Riechelmann H. , Widmann G.
Published: September 2015

Background

Tumor volume may serve as a predictor of response to radiochemotherapy (RCT) in head and neck squamous cell carcinoma (HNSCC). Computer assisted tumor volumetry requires time-consuming slice-by-slice manual or semi-automated segmentation. We questioned how accurately primary tumor and suspect cervical lymph node (LN) volumes can be approximated by the maximum tumor diameters in three dimensions.

Methods

In contrast-enhanced diagnostic CT scans of 74 patients with incident advanced HNSCC, manual slice-by-slice segmentation volumetry of primary tumor, total- and largest suspect cervical LN served as the reference method. In the same scans, maximum orthogonal diameters were measured using the distance measurement tool in standard visualization software in axial and coronal sections. From these diameters, approximate volumes were calculated using the cubic and ellipsoid formula. A second segmentation volumetry was performed in contrast enhanced radiotherapy-planning CT scans obtained prior to primary concurrent RCT 24 days (+/− 13 days) following the initial diagnostic CT scans. Intraclass correlation coefficients and Bland-Altman analyses were used to compare results.

Results

Slice-by-slice manual segmentation volumetry of primary and LN volumes revealed a lognormal distribution and ranged from 0 to 86 ml and 0 to 129 ml, respectively. Volume approximations in diagnostic CT scans with the ellipsoid formula resulted in an −8 % underestimation of tumor volumes (95 % CI −14 % to −1 %; p = 0.022) and an −18 % underestimation of suspect cervical LN volumes (95 % CI −25 % to −12 %; p = 0.001). Inter rater intraclass correlation for primaries was 0.95 (95 % CI +0.92 to +0.97; p = 0.001), and intra rater intraclass correlation was 0.99 (95 % CI +0.98 to +0.99; p = 0.001). The cubic formula resulted in pronounced overestimation of primary and LN volumes. Primary tumor volumes obtained by the second segmentation volumetry in radiotherapy-planning CT scans obtained on average 24 days following the initial volumetry resulted in larger primary tumor volumes (mean bias +28 %, 95 % CI +14 % to +41 %; p = 0.001). Tumor volume increase correlated with time between the diagnostic and planning CTs (r = 0.24, p = 0.05) and was approximately 1 % per day.

Discussion

Ellipsoid approximations of tumor and lymph node volumes in HNSCC using maximum orthogonal diameters underestimates volumes based on segmentation in multiple slices. Due to time difference and safety margins, segmented volumes in radiotherapy-planning CT scans tend to be larger than in diagnostic CT scans.

Conclusion

Ellipsoid approximations of tumor and lymph node volumes in HNSCC are easily available from diagnostic CT scans. Volume estimates are applicable over a wide range of tumor and LN sizes and may be useful in clinical decision-making and oncologic research.
Philippine-US relations: the relevance of an evolving alliance
Authors: Avila Ava Patricia C. , Goldman Justin
Published: September 2015

The Philippines and the United States maintain close ties that are grounded in a Mutual Defense Treaty signed in 1951. Security cooperation has been a hallmark despite evolving dynamics in the bilateral relationship, including a US colonial legacy that continues to cast a long shadow for many Filipinos. While contentious politics and domestic limitations present a potential constraint on the upward trajectory of the alliance relationship, there are indications that this expanded engagement can continue beyond the Aquino administration. The paper examines the condition of Philippine forces under President Aquino, the International Peace and Security Plan to pursue a credible external defense capability, the process of security sector reform, and matters pursuing a strong Philippine-US alliance.
Intratumoral Heterogeneity of ALK-Rearranged and ALK/EGFR Coaltered Lung Adenocarcinoma
Authors: Cai Weijing , Lin Dongmei , Wu Chunyan , Li Xuefei , Zhao Chao , Zheng Limou , Chuai Shannon , Fei Ke , Zhou Caicun , Hirsch Fred R.
Published: September 2015

Purpose

Genetic intratumoral heterogeneity has a profound influence on the selection of clinical treatment strategies and on addressing resistance to targeted therapy. The purpose of this study was to explore the potential effect of intratumoral heterogeneity on both genetic and pathologic characteristics of ALK-rearranged lung adenocarcinoma (LADC).

Methods

We tested ALK fusions and EGFR mutations in 629 patients with LADC by using laser-capture microdissection to capture spatially separated tumor cell subpopulations in various adenocarcinoma subtypes and to test for ALK fusions and EGFR mutations in ALK-rearranged, EGFR-mutated, and ALK/EGFR coaltered LADCs to compare the oncogenic driver status between different tumor cell subpopulations in the same primary tumor.

Results

Among the 629 patients, 30 (4.8%) had ALK fusions, 364 (57.9%) had EGFR mutations, and two had ALK fusions that coexisted with EGFR mutations. Intratumoral heterogeneity of ALK fusions were identified in nine patients by reverse-transcriptase polymerase chain reaction. In the two patients with an ALK/EGFR coaltered status, genetic intratumoral heterogeneity was observed both between different growth patterns and within the same growth pattern. The relative abundance of ALK and EGFR alterations was different in the same captured area. ALK fusions were positively associated with a micropapillary pattern (P = .002) and were negatively associated with a lepidic pattern (P = .008) in an expanded statistical analysis of 900 individual adenocarcinoma components, although they appeared to be more common in acinar-predominant LADCs in the analysis of 629 patients.

Conclusion

Intratumoral genetic heterogeneity was demonstrated to coexist with histologic heterogeneity in both single-driver and ALK/EGFR coaltered LADCs. Altered oncogenic drivers in spatially separated subclones of the same tumor may be different.