bluuebunny/crossref_metadata_2025 huggingface dataset:
Critical Contributions of Pre-S1 Shoulder and Proximal TRP Box in DAG-activated TRPC Channels by PIP2 Regulation
Authors: Mori Masayuki X. , Okada Ryo , Sakaguchi Reiko , Hase Hideharu , Imai Yuko , Polat Onur K. , Itoh Satoru G. , Okumura Hisashi , Mori Yasuo , Okamura Yasushi , Inoue Ryuji
Published: April 2022

Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 or PIP2) regulates the activities of numerous membrane proteins, including diacylglycerol(DAG)-activated TRPC3/6/7 channels. Although PIP2 binding is known to support DAG-activated TRP channel activity, its binding site remains unknown. We screened for PIP2 binding sites within TRPC6 channels through extensive mutagenesis. Using voltage-sensitive phosphatase (DrVSP), we found that Arg437 and Lys442, located in the channel’s pre-S1 domain/shoulder, are crucial for interaction with PIP2. To gain structural insights, we conducted computer protein-ligand docking simulations with the pre-S1 domain/shoulder of TRPC6 channels. Further, the functional significance of PIP2 binding to the pre-S1 shoulder was assessed for receptor-operated channel functions, cross-reactivity to DAG activation, and the kinetic model simulation. These results revealed that basic residues in the pre-S1 domain/shoulder play a central role in the regulation of PIP2-dependent gating. In addition, neutralizing mutation of K771 in the proximal TRP box reversed the effect of PIP2 depletion from inhibiting to potentiating channel activity. A similar effect was seen in TRPV1 channels implies that TRPC6 possesses a common but robust polarity switch mediating the PIP2 binding effect. Overall, these mutagenesis studies reveal functional and structural insights for how basic residues and channel segments in TRP channels are controlled through phosphoinositide recognition.
Comparative study of Rivastigmine and Galantamine on the transgenic Drosophila model of Alzheimer’s Disease
Authors: Siddique Yasir Hasan , Naz Falaq , - Rahul , Varshney Himanshi
Published: April 2022

Alzheimer’s Disease (AD) is now becoming more prevalent in ageing populations worldwide. It is characterized as a progressive neurodegenerative disease most commonly associated with memory deficits and cognitive decline. The formation of amyloid plaques and neurofibrillary tangles are important pathological markers of AD. Cholinesterase inhibitors are widely used to decrease the hydrolysis of acetylcholine released from presynaptic neurons. In the present study we have studied the effect of rivastigmine and galantamine (commonly used cholinesterase inhibitors) on the transgenic Drosophila model of AD expressing human Aβ-42 in the neurons. The effect of similar doses of rivastigmine and galantamine (i.e. 0.1,1 and 10mM) was studied on the climbing ability, lifespan, oxidative stress markers, caspase 9 and 3, acetylcholinesterase activity and on the formation of Aβ-42 aggregates. The results suggest that the rivastigmine is more potent in reducing the oxidative stress and improving climbing ability of AD flies. Galantamine is a more potent inhibitor of acetylcholinesterase compared to rivastigmine. Galantamine prevents the formation of Aβ-42 aggregates more effectively compared to rivastigmine. The present study gives a comparative account of the rivastigmine and galantamine on the efficacy. The effect of the drugs taking the same doses have been studied on the climbing ability, life span, oxidative stress markers, apoptotic markers, acetylcholinesterase activity and on the formation of Aβ-42 aggregates. The study has been performed on the transgenic Drosophila model of AD expressing human Aβ-42 in the neurons.
Ceftazidime/avibactam-based versus polymyxin B-based therapeutic regimens for the treatment of carbapenem-resistant Klebsiella pneumoniae infection in critically ill patients: a retrospective cohort study
Authors: Zheng Guanhao , Cai Jiaqi , Zhang Liang , Chen Dayu , Wang Linyu , Qiu Yusi , Deng Han , Bai Hao , Bian Xiaolan , He Juan
Published: April 2022

Background: Considering the importance of ceftazidime/avibactam (CAZ/AVI) and polymyxin B (PMB) in treating carbapenem-resistant Klebsiella pneumoniae (CRKP) infection, it is essential to evaluate the efficacy and safety of these agents and provide appropriate medical advice to clinical specialists. Methods: We conducted a retrospective cohort study in two Chinese tertiary hospitals for critically ill patients with CRKP infection who received at least 24-hour CAZ/AVI-based or PMB-based treatment. A binary logistic model and a Cox proportional hazards regression model were constructed to analyze variables that could potentially affect 30-day microbiological eradication and all-cause mortality, respectively. Results: From January 2019 to December 2021, 164 eligible patients were divided into CAZ/AVI and PMB cohorts. A notably lower 30-day mortality rate (35.4% vs 69.5%, P < 0.001) and a higher 30-day microbiological eradication rate (80.5% vs 32.9%, P < 0.001) were observed for patients receiving CAZ/AVI-based treatment, compared with cases in PMB group. A longer antimicrobial treatment duration (> 7 days) could also significantly decrease the mortality rate and increase the microbiological eradication rate. Female patients had a higher survival rate than male. Age over 65 years, sepsis, Continuous Renal Replacement Therapy (CRRT) and organ transplantation were identified as negative factors on survival. In the subgroup analysis, we found that CAZ/AVI combined with tigecycline (Hazard ratio, 0.267; 95% Confidence Interval, 0.077-0.921; P = 0.037) or amikacin (Hazard ratio, 0.105; 95% Confidence Interval, 0.014-0.766; P = 0.026) could effectively lower mortality. According to safety evaluation results, potential hepatic enzymes elevation was associated with CAZ/AVI-based treatment, while renal impairment was probably related to PMB-based treatment. Conclusions: CAZ/AVI was more effective than PMB in treating CRKP infected patients. Tigecycline and amikacin were proved to be beneficial as concomitant agents in combination with CAZ/AVI. A treatment period lasting over 7 days was recommended. Although clinical safety of these two agents could be ensured because the severity level and incidence of adverse effects were relatively low, hepatoxicity of CAZ/AVI and nephrotoxicity of PMB should be monitored carefully. Further well-designed studies should be performed to verify our conclusion.
Establishment and validation of a pathologic upgrade prediction nomogram model for gastric low-grade intraepithelial neoplasia patients after the eradication of Helicobacter pylori
Authors: Ruan Yejiao , Lu Guangrong , Zhu Yuesheng , Ma Xianhui , Shen Yating , Shi Yuning , Zhang Xuchao , Zhu Zheng , Cai Zhenzhai , Xia Xuanping
Published: April 2022

Background: At present, there is no unified treatment for the evaluation and management of gastric low-grade intraepithelial neoplasia (LGIN) all over the world.Methods: Patients who were Helicobacter pylori eradicated, with low-grade gastric intraepithelial neoplasia were gathered.Several demographic and clinicopathological characteristics were described and analyzed retrospectively by LASSO regression analysis and multivariable logistic regression. Then the predictive nomogram was established. C-index, the area under the receiver operating characteristic curve (AUC) , calibration plot and decision curve analysis (DCA) were used to evaluate the accuracy and reliability of the model. Results: A total of 309 patients with LGIN were included, divided into training groups and validation groups randomly. LASSO regression analysis and multivariable logistic regression showed that six variables, gender, size, location, border line, number and erosion were independent risk factors for progression of gastric LGIN. The nomogram model displayed good discrimination with a C-index of 0.765 (95% confidence interval: 0.702–0.828). High C-index value of 0.768 could still be reached in the internal validation. The accuracy and reliability of the model was also verified by the AUC of 0.764 in the training group and 0.757 in the validation group. The calibration curve showed the model was in good agreement with the actual results as well. Decision curve analysis suggested that the predictive nomogram had clinical utility. Conclusions: A predictive nomogram model was successfully established and proved to identify high-risk groups with possible pathologic upgrade in patients with gastric LGIN. It suggested that after identifying high-risk patients, strengthening follow-up or endoscopic treatment may benefit in improving the detection rate or reducing the incidence of gastric cancer, which providing a reliable basis for the treatment of LGIN.
Bacillus sp. DU-106 ameliorates anti-diabetic effect by modulating gut microbiota in high-fat-fed and streptozotocin-induced type 2 diabetic mice
Authors: Yan Jing , Li Junjian , Xue Qiuyan , Xie Shiqing , Jiang Jinjin , Li Pan , Du Bing
Published: April 2022

Type 2 diabetes (T2D) is a chronic disease that manifests with endocrine and metabolic disorders, seriously threatening public health. This study aims to investigate the effects of Bacillus sp. DU-106 on anti-diabetic and gut microbiota in C57BL/6J mice on a high-fat diet (HFD) and streptozotocin (STZ)-induced T2D. Bacillus sp. DU-106 was administered to model mice for 8 consecutive weeks. Oral administration of Bacillus sp. DU-106 led to decreased food and water intake and alleviated body weight loss. And Bacillus sp. DU-106 imparted several health benefits to the mice, including balanced blood glucose, alleviation of insulin resistance in the T2D mice, and an improvement in lipid metabolism. Furthermore, Bacillus sp. DU-106 protected against liver and pancreas impairment to some extent. Additionally, Bacillus sp. DU-106 treatment reshaped the intestinal flora by enhancing gut microbial diversity and enriching the abundance of certain functional bacteria. Collectively, these findings suggested that Bacillus sp. DU-106 has the potential to ameliorate T2D by regulation of the gut microbiota. Therefore, a novel probiotic proposed here, Bacillus sp. DU-106 might be a promising therapeutic agent to prevent and alleviate T2D in mice.
Description and interpretation of the lived experience of hematopoietic stem cell transplantation, focusing on before, during, and after transplantation: A hermeneutic phenomenological approach
Authors: Rambod Masoume , Pasyar Nilofar , Zarshenas Ladan , Bagheri Shahpar , Ramzi Mani
Published: April 2022

Purpose: This study aimed at description and interpretation of the lived experience of Hematopoietic Stem Cell Transplantation (HSCT) as a novel therapeutic option in hematologic cancers.Methods: This hermeneutic phenomenological study was conducted on 13 individuals who had undergone HSCT. The participants were selected using purposeful sampling. In-depth interview was used and data analysis was conducted using van Manen’s methodological framework.Results: Three themes emerged in this study, including “bewilderment in the shadow of hope”, “emerging dream of budding”, and “entering the calm beach”. Bewilderment in the shadow of hope consisted of four subthemes, namely “strange and shocking news”, “searching for information accomplished with psychological distress”, “having some misconceptions”, and “finding a chance for survival”. In addition, five subthemes of “chemotherapy as a root of transplantation”, “immersing in the psychological burden”, “shining light of life”, “waiting for successful transplantation”, and “living with positive feelings” emerged from “emerging dream of budding”. Finally, “entering the calm beach” consisted of “resilience in spite of physical burden”, “constant attention to blood cells count”, “conducting careful self-care”, “having family support”, “strong relationship with God”, and “valuable rebirth and continuation of life”.Conclusion: Being informed about the need for HSCT was shocking and led to psychological distress and misconceptions before HSCT performance. However, entrance of stem cells into the body shined the light of life and the participants spent their hospitalization period with positive feelings. After HSCT, psychosocial and spiritual interventions are suggested to be provided to improve calm life among individuals.
Association of PRLR, IGF1 and LEP genes polymorphism with milk production and litter size in Egyptian Zaraibi goat
Authors: El-Shorbagy Haidan Mostafa , Abdel-Aal Ehab Salah , Mohamed Shaimaa Abdel-Azeem , El-Ghor Akmal Abdel-Reheim
Published: April 2022

This study aimed to detect specific nucleotide polymorphisms in Prolactin receptor (PRLR), Insulin like growth factor (IGF1) and Leptin (LEP) genes and their correlation with milk production (MP) and litter size (LS) traits in Zaraibi goat. PCR-SSCP products of different patterns of each gene were sequenced and aligned to reveal two single nucleotide polymorphisms (SNPs) (T>C) and (G>A) in 3’UTR of PRLR gene and registered with accession numbers OM418863 for TT and OM418864 for CT, while (G>A) SNP was registered as OM418861 for GG and OM418862 for AG in exon 10. TT, CT, AG and GG genotypes were distributed in the studied animals with frequencies 0.43, 0.57, 0.65 and 0.35, respectively. While alleles C, T, A and G frequencies were 0.28, 0.72, 0.32 and 0.68, respectively. CT and AG genotypes associated significantly (P<0.05) with higher MP and LS respectively. By studying the haplotypes of PRLR, C-A and T-A were associated with the highest and the lowest level of MP respectively. For LS, T-A and C-G showed significant correlation with the highest and the lowest rate respectively. Regarding IGF1 gene, two SNPs were detected; T74C at exon 4 which registered as (OM418860), and combined SNPs as ins. G470, A531G and T534C (PP genotype) at 5’ flanking region that registered as (OM418859). For LEP, only one SNP was found in intron 2 (G281A) which submitted to GenBank as (OM418855). All detected SNPs have shown to be involved in regulating the MP or LS as reproductive traits in goat.
The Impact of China's Environmental Regulation on Total Factor Productivity of Pharmaceutical Manufacturing Industry: An Analysis Based on the Spatial Spillover Effect
Authors: Wu Qijie , Li Yuexin , Wu Yufei , Li Fei , Zhong Shen
Published: April 2022

As an important embodiment of a country's economic strength and national health, pharmaceutical manufacturing industry has made rapid development in China in recent years. But at the same time, the pharmaceutical manufacturing industry is facing many environmental problems, such as large pollution emissions, complex pollution components, controlling difficulties and so on. This paper measures the total factor productivity of pharmaceutical manufacturing industry (HTFP) by using data envelopment analysis (DEA), and studies the effect of environmental regulation (ERI) on the total factor productivity of pharmaceutical manufacturing industry (HTFP) by establishing panel data regression model and spatial econometric model based on 30 provinces in China from 2004 to 2019. The conclusions are as follows: (1) Environmental regulation and total factor productivity of pharmaceutical manufacturing industry have significant spatial autocorrelation, showing "high-high" or "low-low" spatial aggregation characteristics; (2) Environmental regulation has a significant promoting effect on improving HTFP in local and surrounding areas, and there are differences in the impact of eastern, central and western regions; (3) Green technology, production technology and industrial structure play an important role in the impact of ERI on HTFP, which provides theoretical guidance and policy recommendations for improving the level of total factor productivity of pharmaceutical manufacturing industry in the environmental aspect.
A kinematic precision reliability evaluation method for rotor-bearing systems considering multi-source wear degradations and random errors
Authors: Wang Hongwei , Xiang Jiawei , Zhao Xufeng , Li Yulong
Published: April 2022

Rotor-bearing systems play a vital role in machine tools, aero engines, and wind turbines. Generally, worn-induced degradation quantities and manufacturing errors of components are the main error sources that influence the precision reliability of rotor-bearing systems. The current precision reliability evaluation models are focusing on several error sources in only a few key components without agreeable results. Therefore, a precision reliability evaluation model is proposed considering all time-variant error sources and random error sources. Firstly, time-variant wear models for commonly occurred degradation types in a rotor-bearing system are developed. Secondly, the constructed time-variant wear models are inserted into the precision model with all moving components in the rotor-bearing system using the Meta-Action structural decomposition method. Finally, the time-variant stochastic process discretization method is employed to establish the precision reliability evaluation model, and solve the precision reliability of the rotor-bearing systems. Case investigations are carried out to verify the performance of the present model, which provides a better precision reliability evaluation model to estimate the conditions of rotor-bearing systems during the service period.
Unraveling rationale behind tomato (Solanum Lycopersicum L.) shelf life: Role of fruit biochemical, morpho- physiological traits in extending shelf life
Authors: M.P. Pavan , S. Gangaprasad , Kumar Niranjana , Adivappar Nagarajappa
Published: April 2022

Prolonging the keeping quality of tomatoes is very essential for reduction of great losses in quality and quantity. Fruit shelf life is ripening-associated complex trait affected by many quantitative fruit quality traits. In this study, 30 tomato germplasm lines were characterized for very important less pursued shelf life and its contributing traits. Shelf life ranged from 10.50 to 34.43 days. Slower dehydration rate noticed in tender fruit tissues in turning stage. Slow pace in rate of change in physiological losses in fruit weight led to high shelf life. Fruit firmness ranged from 2.49 to 5.76 kg/cm2 and pericarp thickness from 1.33 to 6.70 mm. High shelf life germplasm lines contained 6.98–19.29 mg/100g and 8.64–13.38 mg/100g of fruit calcium and magnesium content respectively. The fruit epicuticular wax content varied from 34.64 to 96.77 µg/cm2. Calcium, magnesium, and epicuticular wax decrease respiration rate, maintain firmness, delay senescence, reduces incidence of physiological disorders and extend fruit storage life. Higher shelf life in germplasm lines P-4, L-00191, L-03686, AR-23, PKM-1, and Bony best are contributed by higher firmness and epicuticular wax content and high shelf life in Red ball is due to higher firmness, pericarp thickness, calcium, magnesium and epicuticular wax content.
Feeding behaviour of Foot and Mouth Disease (FMD) infected Holstein Friesian crossbred calves offered with therapeutic diet
Authors: Somagond Arun , Patel B. H. Manjunatha , Pattanaik A K , Verma M. R. , Dutt Triveni , Bhanuprakash V.
Published: April 2022

Foot and Mouth Disease (FMD) affects dry matter intake (DMI) and performance due to mucosal erosions developed on the tongue and gums. Therapeutic diets (TD) in a physical form compatible with the inflammatory condition of the mouth ensures optimum intake with changed behavioural pattern. The present study aimed to record the feeding behaviour of FMD infected calves by offering a therapeutic diet. A total of 22 Holstein Friesian crossbred male calves (10–12 months) were used in which 4 calves were of Control (CON) and 18 calves of treatment groups viz. Therapeutic Diet- Mash (TDM), Therapeutic Diet-Cooked (TDC), Therapeutic Diet-Customized Nutrient Supplement (TDCNS) were infected with FMD virus in the bio-containment facility (BSL-III). The therapeutic diet (TD) was fed to calves in the forenoon session and ad libitum hybrid Napier in afternoon session. The diet had high protein and energy with varied physical forms (mash or cooked). The infected group calves experienced difficulty in eating viz. 0.00 ± 0.00, 19.57 ± 3.19, 25.48 ± 4.13 & 27.30 ± 4.38 min in CON, TDM, TDC, TDCNS respectively. The time values were highly significant between the groups (p < 0.01). Licking behaviour was noticed only in the morning session. Feed tossing, ruminating behaviour was noticed in the afternoon session in all the animals irrespective of groups. The frequency of urination was more in the morning and the defecation was comparable between the groups. The lethargy score was high for 3 days post-infection in the infected groups and subsequently declined. Non-nutritive behaviour is not seen either in the forenoon or in the afternoon session. In conclusion, tailor made therapeutic diets with changed physical forms (mash or cooked) was compatible with the inflammatory condition of the mouth.
Implementation and Evaluation of a Discharge Planning for Patients Undergoing Umbilical Cord Blood Transplantation
Authors: Huang Lu , Zhu Yan , Wu Yun , Wang Ying-Ying , Song Gui-qi , Song Kai-di , Wu Yao-hua , Zhang Yong-Liang
Published: April 2022

Purpose
The purpose of this study was to implement a discharge planning for patients undergoing umbilical cord blood transplantation (UCBT) and evaluate the effects on discharge readiness, length of stay, hospital readmission, self-efficacy, and quality of life (QoL).
Methods
Participants (n = 66) were assigned to a control group (CG: received usual care) or an intervention group (IG: received discharge planning). Discharge readiness, length of stay, and the rate of hospital readmission were collected. Additionally, self-efficacy and quality of life survey (FACT-BMT) were assessed at hospital admission (T1), discharge (T2), and approximately Day 100 after UCBT (T3).
Results
The discharge planning group showed significantly better discharge readiness (p < 0.001), improvement in self-efficacy (increased by 11.04% at T3), the time effect (p = 0.035) and interaction effect (p = 0.027) were significant. Except for emotional and social well-being, all the other dimensions and three total scores of FACT-BMT in the IG were higher than controls, the main effect of group was significant (p < 0.05). However, discharge planning did not reduce patients’ length of stay or hospital readmissions.
Conclusion
This study provided the evidences on the implementation of discharge planning for patients undergoing UCBT, and suggested that nurse-led discharge planning should be incorporated into usual care for successful hospital-to-home transitions.
Subacute and chronic proteomic and phosphoproteomic analyses of a mouse model of traumatic brain injury at two timepoints and the comparison with chronic traumatic encephalopathy in human samples.
Authors: Morin Alexander , Roderick Davis , Teresa Darcey , Benoit Mouzon , Crawford Fiona
Published: April 2022

Repetitive mild traumatic brain injury (r-mTBI) is the most widespread type of brain traumas worldwide. The cumulative injury effect triggers long-lasting pathological and molecular changes that may increase a risk of chronic neurodegenerative diseases. R-mTBI is also characterized by changes in brain proteome, where the majority of molecules altered early post-TBI are different from those altered at more chronic phases. This differentiation may contribute to heterogeneity of available data on potential therapeutic targets and may present an obstacle in developing effective treatments. Here, we aimed to characterize a proteome profile of r-mTBI in a mouse model at two time points – 3 and 24 weeks post last TBI, as this may be a more relevant therapeutic window for individuals suffering negative consequences of r-mTBI. We identified a great number of proteins and phosphoproteins that remain continuously dysregulated from 3 to 24 weeks. These proteins may serve as effective therapeutic targets for sub-acute and chronic stages of post r-mTBI. We also compared canonical pathways activation associated with either total proteins or phosphoproteins and revealed that they both are upregulated at 24 weeks. However, at 3 weeks post-TBI, only pathways associated with total proteins are upregulated, while pathways driven by phosphoproteins are downregulated. Finally, to assess the translatability of our data, we compared proteomic changes in our mouse model with those reported in human samples of Chronic Traumatic Encephalopathy (CTE) compared to controls. We observed 39 common proteins that were upregulated in both species and 24 common pathways associated with these proteins. These findings support the translational relevance of our mouse model of r-mTBI for successful identification and translation of therapeutic targets.
Hepatitis B core antibody (HBcAb)-positive is significantly correlated with hilar cholangiocarcinoma and affects operative safety of major hepatectomy in Chinese patients
Authors: Huang Zhiyong , Wang Wenqiang , Xu Guangyuan , Zhang Erlei , Liang Binyong , Li Jiang , Li Jian , Lin Meilong , Chen Xiaoping
Published: April 2022

Background: Hepatitis B virus (HBV) may be a risk factor for cholangiocarcinoma, but it is not yet clear that the relationship between hepatitis B core antibody (HBcAb)-positive and hilar cholangiocarcinoma (hCCA). In addition, we evaluated the impact of HBcAb-positive on the safety of major hepatectomy for hCCA. Methods：A retrospective analysis was performed on the status of hepatitis B infection, liver fibrosis, surgical safety, and long-term postoperative survival of 111 patients who underwent major hepatectomy in Tongji Hospital from April 2012 to September 2019.Results: HBsAg-positive was identified in 10.6%, HBcAb-positive was found in 73.7% of the patients. A high-grade of liver fibrosis was detected in 67.9% of the patients with HBcAb-positive, which was markedly higher than that in the patients with HBcAb-negative (43.3%) (P=0.018). The incidence rates of postoperative complications and 30-day mortality were 38.7% (43/111) and 5.4% (6/111), respectively. Multivariate analysis showed that the independent risk factors for complications were HBcAb, vascular reconstruction, lymphadenectomy, HBcAb-positive correlated with a higher incidence of complications after major hepatectomy for hCCA (adjusted hazard ratio [HR] 3.984, 95%CI 1.354-11.73; P = 0.012). All the patients who died within 30-day after surgery were HBcAb-positive. However, there was no correlation between HBcAb and long-term prognosis of major hepatectomy for hCCA.Conclusions: HBcAb-positive is closely correlated with hCCA. HBcAb-positive markedly increased the incidence of postoperative complications and mortality of major hepatectomy for hCCA.
Clinicopathological Characterization of Small Cell Synovial Sarcoma: A Case Report
Authors: Qin Lingyan , Yang Hongli , Guo Lingchuan , Ma Wenxia , Gu Dongmei
Published: April 2022

Background
As an umbrella term of malignant tumor, synovial sarcoma (SS) is, characterized by bidirectional differentiation and harboring of SYT-SSX1 fusions, with a rare subtype, small cell synovial sarcoma (SCSS), highly malignant. In this paper, we reported a case of SCSS and reviewed related literature.
Case Presentation:
A 39-year-old women visited our hospital due to chest tightness for over two years along with serious cough. The outpatient chest CT scan revealed a mass in the upper right lung. Further examinations such as contrast-enhanced CT and Fiberoptic bronchoscopy at our hospital indicated a malignant lung tumor. Thereby the ultrasound-guided needle biopsy was performed on the patient for further diagnosis. Postoperative histological examination suggested that the tumor cells were oval or short fusiform with variable size. The tumor cells presented the form of nests, with less cytoplasm and weak eosinophilia. The nucleus was round or oval with densely stained chromatin, while naked nuclei identifiable in the background. All these findings indicated inclination to SCSS, which required further confirmed diagnosis by the results of immunohistochemical analysis and FISH tests. The patient underwent periodic chemotherapy after the confirmation of diagnosis, with no recurrence within 36 months of follow-up, which unfortunately, was available as of the 42nd month.
Conclusions
SCSS is a scarce tumor without prominent specificity in terms of clinical manifestations. Diagnosis and differential diagnosis require a combination of pathological characterization, immunophenotyping and molecular genetic testing. The clinical regimens mainly rely on the complete surgical resection of the mass whereas chemotherapy and radiotherapy reportedly improve the prognosis of patients.
Behaviors of Chemical Anchors Installed in Different Types of Concrete
Authors: ÇALIŞKAN Özlem , KARAKURT Cenk , ARAS Murat , KAYA Turgut
Published: April 2022

Chemical anchors are widely used in additional installations of construction irons, repair and reinforcement work. Due to the increasing diversity of materials and the improved awareness of waste products, it has become possible to produce different types of concrete with various characteristics. In this study, class B420C ribbed bars with 16 mm diameter were installed in four different concrete types by using four different chemical adhesives, and a tensile force was applied. The stiffness, displacement ductility ratio, energy-absorbing capability and tensile force values were determined and the failure modes were interpreted from the load-displacement curves obtained as a result of the experiments. It was observed that tensile force and energy-absorbing capacity had increased as a result of installing anchor and applying a tensile force to the concrete that was obtained by adding admixture materials to the reference concrete. In the analytical part of the study, the formulation provided in ACI 318 was used, capacity and design strengths were identified, and their safety levels were determined in comparison with the test results.
Three-year outcomes of ophthalmological examinations performed at 0–3 months of term/preterm infants
Authors: Süt Nurşah Yeniay , Çelik Gökhan , Kızılay Osman , Karatekin Güner , Büyükkayhan Derya
Published: April 2022

Purpose: To present the ocular pathologies detected in babies with gestational ages >34 weeks in the ophthalmology outpatient clinic, as well as determine the frequency of these pathologies and their relationship with various variables, using data covering a span of three years. Materials: The outcomes of the vision screening performed in 7,820 term/preterm infants aged 0–3 months who were examined at the ophthalmology outpatient clinic of Zeynep Kamil Maternity and Children’s Diseases Training and Research Hospital between 2015 and 2017 were analyzed in this retrospective, cross-sectional, observational, and descriptive study. Results: A total of 7,820 term and late-preterm infants were examined. An ocular pathology was identified in 20.1% (n=1,569) of the infants. Retinal hemorrhage was detected as the most common pathology in 9.5% (n=740) of the infants, and it was more common in infants who were ≤28 days than those who were >28 days (p<0.001). Immature vascularization (4.4%; n=346) and conjunctivitis (4.0%; n=313) were the other more common ocular pathologies. Conclusion: Abnormal ocular findings, including disorders that could be components of a systemic disease and could cause loss of vision, were identified in 20.1% of the infants. The first three months of life comprises the period of visual function evolution. Therefore, an ophthalmological examination should be performed on all newborns before discharge or as soon as possible after discharge for the early diagnosis and timely management of abnormal findings.
Increased expression of leucine-rich α-2 glycoprotein 1 as a predictive biomarker of favorable progression-free survival in meningioma
Authors: Furuta Takuya , Moritsubo Mayuko , Miyoshi Junko , Komaki Satoru , Sakata Kiyohiko , Miyoshi Hiroaki , Morioka Motohiro , Ohshima Koichi , Sugita Yasuo
Published: April 2022

Purpose Most meningiomas, which are frequent central nervous system tumors, are classified as WHO grade 1 because of their slow growing nature. However, the recurrence rate varies and is difficult to predict using conventional histopathological diagnoses. Leucine-rich α-2 glycoprotein 1 (LRG1) is involved in cell signal transduction, cell adhesion, and DNA repair and is a predictive biomarker in different malignant tumors; however, such a relationship has not been reported in meningiomas. Methods/patients We examined tissue microarrays of histological samples from 121 patients with grade I and grade II meningiomas and assessed their clinical and pathological features, including expression of LRG1 protein. Results LRG1-high meningiomas showed increased number of vessels with CD3 positive cell infiltration (p=0.0453) as well as higher CD105 positive vessels (p=0.0041), as compared to LRG1-low cases. They also demonstrated better progression-free survival [hazard ratio (HR) 0.24, 95 % confidence interval (CI) 0.056-0.99] compared to LRG1-low patients (p=0.0317). Moreover, multivariate analysis indicated that high LRG1 expression was an independent prognostic factor (HR, 0.22; CI, 0.05-0.95; p=0.0426). Conclusions LRG1 immunohistochemistry may be a convenient tool for estimating the prognosis of meningiomas in routine practice. Further studies are required to elucidate the key role of LRG1 in meningioma progression.
MIKS - ein inklusives Professionalisierungs- und Schulentwicklungskonzept im Handlungsfeld Mehrsprachigkeit
Authors: Lange Imke
Published: April 2022

Das MIKS-Konzept ist ein Konzept für die Schulentwicklungsarbeit mit Grundschulkollegien, die alle Sprachen ihrer Schüler*innen wertschätzen und für das Lernen nutzen wollen. Ziel ist die Professionalisierung und Qualifizierung des Personals und die Unterstützung von Schulentwicklungsprozessen. Die Grundschulkollegien entwickeln und erproben eigene Praxisvorhaben zum Einbezug von Mehrsprachigkeit, implementieren sie in die regulären Schul- und Unterrichtsabläufe und gestalten so die Schulentwicklung im Handlungsfeld Mehrsprachigkeit selbst.

Das MIKS-Konzept enthält Vorgaben über den Ablauf und die Methoden der Qualifizierung und Schulbegleitung sowie über die Inhalte, die während der Qualifizierung vermittelt und bearbeitet werden. Das MIKS-Konzept wurde 2013-2016 entwickelt und in drei Grundschulen erprobt. Im Rahmen einer Dissemination wurde das Konzept 2016-2019 durch Multiplikator*innen in die Breite getragen und an weiteren 17 Grundschulen durchgeführt. Der Artikel fokussiert zunächst die theoretische Fundierung und Konzeptionalisierung von MIKS. Im zweiten Teil werden die Inhalte, die Anlage und der Ablauf der Qualifizierung erstmals ausführlich vorgestellt und durch Einblicke in bisherige Erfahrungen illustriert.Abstract

The MIKS concept aims at school development and is carried out in elementary schools, which want to value all their students' languages and use them for learning. The aim is the professionalisation and qualification of the staff and the support of school development processes. The primary school colleges develop and test their own multilinguals teaching approaches and implement them in the everyday school routines. The concept includes the transfer of knowledge (psycholinguistic and sociolinguistic foundations), trial phases in lessons (via the implementation of multilingual teaching approaches), and guided reflection exercises concerning the experiences and beliefs among the participating staff.

The MIKS concept was developed in 2013-2016 and was carried out in three elementary schools. As part of a dissemination, the concept 2016-2019 was broadened by multipliers and carried out at further 17 elementary schools. The article focuses on the theoretical foundation and conceptualisation of MIKS. In the second part the contents and the course of the MIKS concept are presented in detail for the first time.
„Was könnte denn das Kind haben?“ Dynamiken der Kooperation von Kindertagesbetreuung und Frühförderung im Kontext inklusionsbezogener Professionalisierung
Authors: Hamacher Catalina , Seitz Simone
Published: April 2022

Auf der Basis einer einführenden Darlegung des Diskurses zur Qualifizierung für inklusive Bildung fokussiert der Beitrag das professionalisierungsbezogene Anliegen der Kooperation von Kindertagesbetreuung und Frühförderung. Es werden ausgewählte Befunde aus dem qualitativen Teil einer abgeschlossenen Untersuchung (mixed methods) zur inklusionsförderlichen Kooperation von Kindertageseinrichtungen und Frühförderstellen herangezogen. Dabei kommt Fallkonstitutionen eine spezifische Bedeutsamkeit zu. Die Befunde werden vor dem Hintergrund von divergenten Orientierungsrahmen und Beauftragungen vertiefend diskutiert, worüber sich ein neuer Blick auf die ungleichheitsbezogene Bedeutsamkeit der Kooperationsprozesse eröffnet. Die Überlegungen werden abschließend zu professionalisierungs- und forschungsbezogenen Implikationen geführt.Abstract

On the basis of an introductory presentation of the discourse on qualification for inclusive education, the article focuses on the professionalisation-related concern of collaboration between day care and early intervention. Reference is made to selected findings from the qualitative part of a completed study (mixed methods) on the inclusion-related collaboration of professionals in Early Education and Early Intervention whereby case constellations have a specific significance. The findings are discussed in terms of divergent orientation frameworks and mandates, which opens up a new perspective on the inequality related significance of collaboration. Finally, the reflections will lead to professionalisation- and research-related implications.
Enstrophy without boost symmetry
Authors: Pinzani-Fokeeva Natalia , Yarom Amos
Published: April 2022

We construct an approximately conserved current for $2+1$ dimensional, Aristotelian (non boost invariant), fluid flow. When Aristotelian symmetry is enhanced to Galilean symmetry, this current matches the enstrophy current responsible for the inverse cascade in incompressible fluids. Other enhancements of Aristotelian symmetry discussed in this work include Lorentzian, Carrollian and Lifshitz scale symmetry.
Balanced partial entanglement and mixed state correlations
Authors: Camargo Hugo A. , Nandy Pratik , Wen Qiang , Zhong Haocheng
Published: April 2022

Recently in Ref. [1], one of the authors introduced the balanced partial
entanglement (BPE), which has been proposed to be dual to the
entanglement wedge cross-section (EWCS). In this paper, we explicitly
demonstrate that the BPE could be considered as a proper measure of the
total intrinsic correlation between two subsystems in a mixed state. The
total correlation includes certain crossing correlations, which are
minimized by particular balance conditions. By constructing a class of
purifications from Euclidean path-integrals, we find that the balanced
crossing correlations show universality and can be considered as the
generalization of the Markov gap for the canonical purification. We also
test the relation between the BPE and the EWCS in three-dimensional
asymptotically flat holography. We find that the balanced crossing
correlation vanishes for the field theory invariant under
BMS_33
symmetry (BMSFT) and dual to the Einstein gravity, indicating the
possibility of a perfect Markov recovery. We further elucidate these
crossing correlations as a signature of tripartite entanglement and
explain their interpretation in both AdS and non-AdS holography.
Evaluation of biomarkers in the studies of keloid tissue after laser therapy
Authors: Tikhomirov A. L. , Kazenashev V. V.
Published: April 2022

The main metabolite of lactobacilli, regulating their anti-inflammatory functions, is lactic acid (LA), which maintains the physiological state of the microbiocenosis of the vagina of women of reproductive age. Normocenosis of the vagina is based on a combination of acidic environment and colonization resistance. Pathogenic and conditionally pathogenic pathogens are colonized mainly in  an alkaline environment. LA is an important biological substrate that supports the  vaginal microbiota. Normally, the reaction of the vaginal contents is a pH of 3.8 to 4.5, and only in this case lactobacilli predominate in the biotope, which are able to unite into special communities – biofilms, forming the most powerful factor in protecting the vagina from adhesion and invasion of pathogens: a full-fledged lactobacillary biofilm is often able to prevent adhesion of even STI pathogens. Disruption of the functioning of each of the links of protection of the vaginal microbiome leads to a change in the pH of the habitat and excessive reproduction of conditionally pathogenic microorganisms. A frequent infectious syndrome, accompanied by an imbalance of the vaginal microbiota and the replacement of lactoflora with various types of anaerobic microorganisms (Gardnerella vaginalis, Ureaplasma urealyticum, Mycoplasma hominis, Mobiluncus, Prevotella), is bacterial vaginosis. When prescribing antibacterial treatment, side effects are possible: allergic reactions, hepatotoxic, nephrotoxic, candidiasis. The review presents the possibility of using LA, which is a basis in the composition of the drug Lactodepantol (Femilex®) – vaginal suppositories containing 100 mg of LA, as the main factor regulating the natural composition of the vaginal microbiota and the possibility of using vaginal suppositories based on LA (lactodepantol) for the prevention and treatment of bacterial vaginosis in the form of monotherapy and in combination with antimicrobials.
Awareness of young women about emergency contraception in the temporal aspect
Authors: Sviridova N. I. , Tkachenko L. V. , Gritsenko I. A.
Published: April 2022

Introduction. Bearing in mind functional features of the reproductive system at every stage of a woman’s life, as it grows and develops, accomplishes its purpose and early fades away, its high vulnerability becomes obvious. Therefore, what emerges is the need for a broader analysis of the causes of reproductive dysfunction, among which termination of unwanted pregnancies plays one of the leading roles.

Aim. To assess the level of awareness of emergency contraception among young women.

Materials and methods. We conducted an anonymous voluntary survey on the awareness of the emergency contraception methods among different age groups of young people in 2014 and 2021. In 2014, 577 respondents participated in the survey, which were divided into two groups. Group 1 included 265 adolescent girls aged 15 to 17 years, group 2 included young women aged 18 to 25 years. In 2021, an anonymous voluntary survey was only conducted among 348 young female respondents.

Results. Analysis of responses from the survey of adolescents and young girls conducted in 2014 shows an extremely low level of awareness of the emergency contraception methods among respondents in both groups. Thus, 211/265 (79.62%) in the group of adolescent girls and 201/312 (64.42%) young women did not know anything about the emergency contraception methods, and 19/312 (6.09%) young women and 9/265 (3.40%) adolescent girls claimed knowledge of the possibilities of emergency contraception using Escapelle. The analysis of a similar survey conducted in 2021 showed that young women’s awareness of modern emergency contraceptive methods had increased. Thus, less than half of 168/348 (48.28%) participants did not know anything about the emergency contraception methods in 2021, and 117/348 (33.62%) respondents were aware of Escapelle, which was 5.5 times more compared with those of 2014.

Conclusions. The results of two surveys we conducted reveal insufficient awareness of the emergency contraceptive methods among young women.
Handheld Micro tiller time-frequency characteristic and vibration isolation measures
Authors: Liu Xiaochan , Wang Yunxiao , Zhang Xiuli , Tian Hui , Yu Fan
Published: April 2022

The handheld micro tiller is a typical small agricultural machine. It vibrates severely during the rotary tillage process, which harms the users’ health. It is necessary to study its vibration characteristics and propose vibration isolation measurements. Firstly, the external forces applied on various components of the micro-tiller were analyzed. The main components of the handheld micro tiller were simplified. And then the micro-tiller vibration model was built. The time-frequency domain characteristics of the micro-tiller were analyzed. The main components’ RMS values were calculated. It shows that the excitation force of the engine is the main source of vibration of the micro-tiller. The simulated RMS values of the frame and the gearbox were compared to the tested values. The errors indicate the established model is to some extent reasonable. In order to reduce the vibration transmitted to the handrail, firstly, the ordinary bolts connecting the engine and the frame were replaced with rubber bolts. And then the RMS value was analyzed and calculated. The results showed that this method could not effectively reduce the vibration of other main components. Furthermore, it is considered to install a vibration isolator between the engine and the frame of the tiller. Time-frequency domain simulation results show that after adding the vibration isolator, the vibration RMS value of each component was much smaller than before. It shows that it isolates the engine vibration effectively. The research in this paper laid the foundation for designing a reasonable vibration isolator and reducing the vibration of the main components of the tiller.
Robot device for ankle joint rehabilitation: a review
Authors: Zhetenbayev Nursultan , Zhauyt Algazy , Balbayev Gani , Shingissov Beibit
Published: April 2022

The purpose of this review document is to summarize the latest developments and research in the field of robotics related to the field of ankle joint rehabilitation, review new results, and determine the actual state of the art. The literature search was carried out using scientific and medical databases and other websites related to robots used in the field of ankle rehabilitation, analyzing research from the 1950s to the present.
Motion simulation and impact gap verification of a wheeled vibration-driven robot for pipelines inspection
Authors: Korendiy Vitaliy , Kachur Oleksandr , Gursky Volodymyr , Kotsiumbas Oleh , Dmyterko Petro , Nikipchuk Serhij , Danylo Yaroslav
Published: April 2022

Vibration-driven locomotion systems and mobile robots are widely used in different industries, in particular, for inspecting and monitoring the pipelines. Among the great variety of such robots designs, the ones based on the wheeled chassis and equipped with the vibratory drive are of the most widespread. The novelty of the present paper consists in substantiating the design parameters of the wheeled robot working under the vibro-impact conditions and driven by the crank-slider excitation mechanism. The main goal of this research is maximizing the robot average speed. While simulating the robot dynamic behavior, the numerical methods are used, in particular, the finite-element methods and the Runge-Kutta methods, which are implemented in the SolidWorks and MapleSim software. The obtained results presented in the form of time dependencies of the robot kinematic and dynamic characteristics can be of significant practical interest for the researchers and designers of the similar robotic systems.