bluuebunny/crossref_metadata_2025 huggingface dataset:
Locating induced earthquakes with a network of seismic stations in Oklahoma via a deep learning method
Authors: Zhang Xiong , Zhang Jie , Yuan Congcong , Liu Sen , Chen Zhibo , Li Weiping
Published: February 2020

The accurate and automated determination of small earthquake (ML < 3.0) locations is still a challenging endeavor due to low signal-to-noise ratio in data. However, such information is critical for monitoring seismic activity and assessing potential hazards. In particular, earthquakes caused by industrial injection have become a public concern, and regulators need a solid capability for estimating small earthquakes that may trigger the action requirements for operators to follow in real time. In this study, we develop a fully convolutional network and locate earthquakes induced during oil and gas operations in Oklahoma with data from 30 network stations. The network is trained by 1,013 cataloged events (ML ≥ 3.0) as base data along with augmented data accounting for smaller events (3.0 > ML ≥ 0.5), and the output is a 3D volume of the event location probability in the Earth. The prediction results suggest that the mean epicenter errors of the testing events (ML ≥ 1.5) vary from 3.7 to 6.4 km, meeting the need of the traffic light system in Oklahoma, but smaller events (ML = 1.0, 0.5) show errors larger than 11 km. Synthetic tests suggest that the accuracy of ground truth from catalog affects the prediction results. Correct ground truth leads to a mean epicenter error of 2.0 km in predictions, but adding a mean location error of 6.3 km to ground truth causes a mean epicenter error of 4.9 km. The automated system is able to distinguish certain interfered events or events out of the monitoring zone based on the output probability estimate. It requires approximately one hundredth of a second to locate an event without the need for any velocity model or human interference.
Rapid and Precise Semi-Automatic Axon Quantification in Human Peripheral Nerves
Authors: Engelmann S. , Ruewe M. , Geis S. , Taeger C. D. , Kehrer M. , Tamm E. R. , Bleys R. L. A. W , Zeman F. , Prantl L. , Kehrer A.
Published: February 2020

We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification of 129 (10.4%) randomly selected biopsies. The software-based method demonstrated a sensitivity of 94% and a specificity of 87%. Semi-automatic axon counting was significantly faster (p < 0.001) than manual counting. It took 1 hour and 47 minutes for all 129 biopsies (averaging 50 sec per biopsy, 0.04 seconds per axon). The counting process is automatic and does not need to be supervised. Manual counting took 21 hours and 6 minutes in total (average 9 minutes and 49 seconds per biopsy, 0.52 seconds per axon). Our method showed a linear correlation to the manual counts (R = 0.944 Spearman rho). Attempts have been made by several research groups to automate axonal load quantification. These methods often require specific hard- and software and are therefore only accessible to a few specialized laboratories. Our semi-automated axon quantification is precise, reliable and time-sparing using publicly available software and should be useful for an effective axon quantification in various human peripheral nerves.
Expression of protocadherin-20 in mouse taste buds
Authors: Hirose Fumie , Takai Shingo , Takahashi Ichiro , Shigemura Noriatsu
Published: February 2020

Taste information is detected by taste cells and then transmitted to the brain through the taste nerve fibers. According to our previous data, there may be specific coding of taste quality between taste cells and nerve fibers. However, the molecular mechanisms underlying this coding specificity remain unclear. The purpose of this study was to identify candidate molecules that may regulate the specific coding. GeneChip analysis of mRNA isolated from the mice taste papillae and taste ganglia revealed that 14 members of the cadherin superfamily, which are important regulators of synapse formation and plasticity, were expressed in both tissues. Among them, protocadherin-20 (Pcdh20) was highly expressed in a subset of taste bud cells, and co-expressed with taste receptor type 1 member 3 (T1R3, a marker of sweet- or umami-sensitive taste cells) but not gustducin or carbonic anhydrase-4 (markers of bitter/sweet- and sour-sensitive taste cells, respectively) in circumvallate papillae. Furthermore, Pcdh20 expression in taste cells occurred later than T1R3 expression during the morphogenesis of taste papillae. Thus, Pcdh20 may be involved in taste quality-specific connections between differentiated taste cells and their partner neurons, thereby acting as a molecular tag for the coding of sweet and/or umami taste.
A rare subpopulation of melanoma cells with low expression of metastasis suppressor NME1 is highly metastatic in vivo
Authors: Snyder Devin , Wang Ying , Kaetzel David M.
Published: February 2020

Despite recent advances in melanoma treatment, metastasis and resistance to therapy remain serious clinical challenges. NME1 is a metastasis suppressor, a class of proteins which inhibits metastatic spread of cancer cells without impact on growth of the primary tumor. We have identified a rare subpopulation of cells with markedly reduced expression of NME1 (NME1LOW) in human melanoma cell lines. To enable isolation of viable NME1LOW cells for phenotypic analysis by fluorescence-activated cell sorting (FACS), a CRISPR-Cas9-mediated approach was used to attach an EGFP coding module to the C-terminus of the endogenous NME1 gene in melanoma cell lines. NME1LOW cells displayed enhanced collective invasion in vitro when implanted as 3D aggregates in Matrigel. NME1LOW cells were also highly metastatic to lung and liver when xenografted subcutaneously in immune-deficient NSG mice. RNA-seq analysis revealed that NME1LOW cells express elevated levels of genes associated with tumor aggressiveness, as well as with morphogenesis of tissues of neural crest-like origin (melanocytes and neurons, bone and heart tissues; GO: 0009653). The highly malignant NME1LOW variant of melanoma cells has potential to provide novel therapeutic targets and molecular markers for improved clinical management of patients with advanced melanoma.
Petroleum-Tolerant Rhizospheric Bacteria: Isolation, Characterization and Bioremediation Potential
Authors: Viesser Jéssica Aparecida , Sugai-Guerios Maura Harumi , Malucelli Lucca Centa , Pincerati Marcia Regina , Karp Susan Grace , Maranho Leila Teresinha
Published: February 2020

Petroleum is an important energy source. Due to its intensive exploration, accidents resulting in oil spills on soil are frequent, which creates consequences to ecosystems and human health. Rhizodegradation is an efficient technique that promotes the decontamination of polluted environments through the selection and use of rhizosphere microorganisms from phytoremediation plants. The aim of this study was to isolate, identify and characterize bacteria capable of degrading petroleum from the rhizosphere ofPanicum aquaticumPoir., a plant that grows in petroleum contaminated soils. Three bacteria were isolated and characterized at the morphological (Gram staining), molecular (16S rRNA gene sequence analysis) and biochemical level. These bacteria were identified as new strains ofBacillus thurigiensis,Bacillus pumilusandRhodococcus hoagii, which have been reported as potential bioremediators in the literature. All three bacteria were able to use petroleum hydrocarbons as the sole carbon source during in vitrodegradation assays. Gas chromatography analysis of these assays indicated reductions of petroleum hydrocarbons between 23% and 96% within 48 h. Among the isolated bacteria,Rhodococcus hoagiipresented the highest efficiency of petroleum consumption, reaching 87% of degradation after only 24 h of cultivation, which corresponds to a higher and faster degradation than previously reported, confirming the potential use ofRhodococcus hoagiifor petroleum biodegradation.
Optimal Mass Transport with Lagrangian Workflow Reveals Advective and Diffusion Driven Solute Transport in the Glymphatic System
Authors: Koundal Sunil , Elkin Rena , Nadeem Saad , Xue Yuechuan , Constantinou Stefan , Sanggaard Simon , Liu Xiaodan , Monte Brittany , Xu Feng , Van Nostrand William , Nedergaard Maiken , Lee Hedok , Wardlaw Joanna , Benveniste Helene , Tannenbaum Allen
Published: February 2020

The glymphatic system (GS) hypothesis states that advective driven cerebrospinal fluid (CSF) influx from the perivascular spaces into the interstitial fluid space rapidly transport solutes and clear waste from brain. However, the presence of advection in neuropil is contested and solutes are claimed to be transported by diffusion only. To address this controversy, we implemented a regularized version of the optimal mass transport (rOMT) problem, wherein the advection/diffusion equation is the only a priori assumption required. rOMT analysis with a Lagrangian perspective of GS transport revealed that solute speed was faster in CSF compared to grey and white matter. Further, rOMT analysis also demonstrated 2-fold differences in regional solute speed within the brain. Collectively, these results imply that advective transport dominates in CSF while diffusion and advection both contribute to GS transport in parenchyma. In a rat model of cerebral small vessel disease (cSVD), solute transport in the perivascular spaces (PVS) and PVS-to-tissue transfer was slower compared to normal rats. Thus, the analytical framework of rOMT provides novel insights in the local dynamics of GS transport that may have implications for neurodegenerative diseases. Future studies should apply the rOMT analysis approach to confirm GS transport reductions in humans with cSVD.
Protective effect of Polygonatum sibiricum Polysaccharide on D-galactose-induced aging rats model
Authors: Zheng Shaoyan
Published: February 2020

The polysaccharide of Polygonatum sibiricum (PSP)is one of the main active ingredients of Polygonatum Polygonatum in Liliaceae. It has anti-tumor, anti-aging, immune regulation, and anti-oxidative effects. Recent studies have shown that the Klotho gene and fibroblast growth factor-23 (FGF-23) have a common receptor, which is closely related to aging and highly expressed in kidney and meninges. Our study aimed to investigate the anti-aging effect of PSP on D-galactose-induced rats and its mechanism. D-galactose (120 mg Kg-1) and PSP (100 mg Kg-1) was used to intervene in rats, respectively. Then The changes of indexes of the natural aging-like model rats before and after PSP intervention were observed. We found that PSP could significantly improve the learning and memory abilities of rats and reverse the pathological changes of kidney tissues in rats. At the same time, PSP up-regulated the expression of Klotho mRNA and Klotho protein in the renal cortex, down-regulated the expression of FOXO3a mRNA and p-FOXO3a protein in renal tissue, and inhibited the expression of FGF-23 protein in the femur. Our studies suggest that PSP may play a role by regulating the Klotho-FGF23 endocrine axis, alleviating oxidative stress, and balancing calcium and phosphorus metabolism.
Author Correction: Recombinant Incretin-Secreting Microbe Improves Metabolic Dysfunction in High-Fat Diet Fed Rodents
Authors: Ryan Paul M. , Patterson Elaine , Kent Robert M. , Stack Helena , O’Connor Paula M. , Murphy Kiera , Peterson Veronica L. , Mandal Rupasri , Wishart David S. , Dinan Timothy G. , Cryan John F. , Seeley Randy J. , Stanton Catherine , Ross R. Paul
Published: February 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Author Correction: Athena: Automated Tuning of k-mer based Genomic Error Correction Algorithms using Language Models
Authors: Abdallah Mustafa , Mahgoub Ashraf , Ahmed Hany , Chaterji Somali
Published: February 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency
Authors: Wang Chie-Hong , Chang Cheng-Hsuan , Lin Tsung-Li , Fu Ru-Huei , Huang Yu-Chuen , Chen Shih-Yin , Shyu Woei-Cherng , Liu Shih-Ping
Published: February 2020

Maintaining the pluripotency of either embryonic stem (ES) cells or induced pluripotent stem (iPS) cells is a fundamental part of stem cell research. In this study, we reported that cordycepin promoted the expression of pluripotency markers in ES and iPS cells. ES cells treated with cordycepin demonstrated their potential for generating embryoid bodies and differentiating into all three germ layers. The expression levels of phospho-Jak2, phospho-Stat3, integrin αV, and integrin β5 were increased after cordycepin treatment. Furthermore, the protein expression levels of IL-6 family proteins (IL-6, IL-11, LIF, oncostatin M (OSM), ciliary neurotrophic factor (CNTF)), and epidermal growth factor (EGF) were also upregulated after cordycepin treatment, but were restored after co-treatment with a Jak2 inhibitor (AG490). The gene expression levels of Yamanaka factors were upregulated in mouse embryonic fibroblasts (MEFs) after cordycepin treatment. Moreover, the generation efficiencies of iPS cells were elevated after cordycepin treatment. We found that iPS cells generated after cordycepin treatment, not only expressed pluripotency markers, but also showed the ability of differentiating into neuron stem/progenitor cells. Taken together, we demonstrated that cordycepin maintained the pluripotency of stem cells via regulation of extracellular matrix (ECM) and Jak2/Stat3 signaling pathway and improved the generation efficiency of iPSCs.
Comparison of the complete plastomes and the phylogenetic analysis of Paulownia species
Authors: Li Pingping , Lou Gongli , Cai Xiaoran , Zhang Bin , Cheng Yueqin , Wang Hongwei
Published: February 2020

Paulownia species are important ecological, economic and ornamental species, but their phylogenetic relationship remains unclear, which seriously affects the development and utilization of these important resources. The complete chloroplast genomes of six Paulownia species were assembled by next-generation sequencing data. By adding two known Paulownia chloroplast genomes to these six assembled genomes, we performed the comparative analysis and phylogenetic tree reconstruction of Paulownia. The results indicated that the chloroplast genomes of Paulownia species ranged in size from 154,107 to 154,694 bp. These chloroplast genomes contained 117 unique functional genes, including 80 protein-coding genes, four rRNA genes, and 33 tRNA genes. Twelve hotspot regions, five protein-coding genes and seven noncoding regions, were identified in the chloroplast genomes that showed high levels of sequence variation. Additionally, positive selection was observed in three genes, rps2, rbcL and ndhG. The maximum likelihood (ML) and Bayesian (BI) analysis strongly supported the monophyletic origin of Paulownia species, which clustered into two major clades: One clade included P. coreana, P. tomentosa and P. kawakamii, while the other clade comprised the 5 other species including P. fargesii and P. australis. This study provides useful genetic information for phylogenetic reconstruction, taxonomic discrepancies, and studying species evolution and phylogeography in Paulownia.
Automated assessment of regional muscle volume and hypertrophy using MRI
Authors: Mandić Mirko , Rullman Eric , Widholm Per , Lilja Mats , Dahlqvist Leinhard Olof , Gustafsson Thomas , Lundberg Tommy R.
Published: February 2020

This study aimed to validate a fully automatic method to quantify knee-extensor muscle volume and exercise-induced hypertrophy. By using a magnetic resonance imaging-based fat-water separated two-point Dixon sequence, the agreement between automated and manual segmentation of a specific ~15-cm region (partial volume) of the quadriceps muscle was assessed. We then explored the sensitivity of the automated technique to detect changes in both complete and partial quadriceps volume in response to 8 weeks of resistance training in 26 healthy men and women. There was a very strong correlation (r = 0.98, P < 0.0001) between the manual and automated method for assessing partial quadriceps volume, yet the volume was 9.6% greater with automated compared with manual analysis (P < 0.0001, 95% limits of agreement −93.3 ± 137.8 cm3). Partial muscle volume showed a 6.0 ± 5.0% (manual) and 4.8 ± 8.3% (automated) increase with training (P < 0.0001). Similarly, the complete quadriceps increased 5.1 ± 5.5% with training (P < 0.0001). The intramuscular fat proportion decreased (P < 0.001) from 4.1% to 3.9% after training. In conclusion, the automated method showed excellent correlation with manual segmentation and could detect clinically relevant magnitudes of exercise-induced muscle hypertrophy. This method could have broad application to accurately measure muscle mass in sports or to monitor clinical conditions associated with muscle wasting and fat infiltration.
Author Correction: 3D Printed Polyvinyl Alcohol Tablets with Multiple Release Profiles
Authors: Xu Xiaowen , Zhao Jingzhou , Wang Maonan , Wang Liang , Yang Junliang
Published: February 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Author Correction: Insufficient production of IL-10 from M2 macrophages impairs in vitro endothelial progenitor cell differentiation in patients with Moyamoya disease
Authors: Nagata Eiichiro , Masuda Haruchika , Nakayama Taira , Netsu Shizuka , Yuzawa Hiroko , Fujii Natsuko , Kohara Saori , Sorimachi Takatoshi , Osada Takahiro , Imazeki Ryoko , Matsumae Mitsunori , Asahara Takayuki , Takizawa Shunya
Published: February 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Acid mediated coupling of aliphatic amines and nitrosoarenes to indoles
Authors: Roy Subhra Kanti , Purkait Anisha , Aziz Sk Md Tarik , Jana Chandan K.
Published: February 2020

Synthesis of indoles from the reaction of amines and nitrosoarenes.
Effect of copper salts on hydrothermal oxidative decarboxylation: a study of phenylacetic acid
Authors: Fu Xuan , Jamison Megan , Jubb Aaron M. , Liao Yiju , Aspin Alexandria , Hayes Kyle , Glein Christopher R. , Yang Ziming
Published: February 2020

Efficient and selective effects of copper salts on hydrothermal oxidative decarboxylation have been discovered and studied.
Mechanism of photochromic transformations and photodegradation of an asymmetrical 2,3-diarylcyclopentenone
Authors: Oplachko Maria V. , Smolentsev Artem B. , Magin Ilya M. , Pozdnyakov Ivan P. , Nichiporenko Vladislav A. , Grivin Vjacheslav P. , Plyusnin Victor F. , Vyazovkin Vladimir V. , Yanshole Vadim V. , Parkhats Marina V. , Yadykov Anton V. , Shirinian Valerii Z. , Glebov Evgeni M.
Published: February 2020

The mechanistic study of photochromic properties and photodegradation of an asymmetrical 2,3-diarylcyclopentenone bearing thiophene and benzothiophene units has been performed.
Spectroscopic evidence of a particular intermolecular interaction in iodomethane–ethanol mixtures: the cooperative effect of halogen bonding, hydrogen bonding, and the solvent effect
Authors: Yao Fei , Gong Nan , Fang Wenhui , Men Zhiwei
Published: February 2020

The particular intermolecular interaction of an iodomethane–ethanol mixture is revealed by NIR, Raman, DFT calculation, and 2D correlation analysis.
Surface plasmon resonance study of the interaction of N-methyl mesoporphyrin IX with G-quadruplex DNA
Authors: Perenon M. , Bonnet H. , Lavergne T. , Dejeu J. , Defrancq E.
Published: February 2020

Surface plasmon resonance (SPR) was used to investigate the interaction between N-methyl mesoporphyrin IX (NMM) and different G-quadruplex (G4) topologies.
Activity adaptability of a DhHP-6 peroxidase-mimic in wide pH and temperature ranges and solvent media
Authors: Yan Jiaqing , Li Zhengqiang , Liu Min , Sun Xiaoli , Ma Li , Wang Zhi , Zhao Zijian , Huang Xuri , Yuan Long
Published: February 2020

Deuterohemin-β-Ala-His-Thr-Val-Glu-Lys (DhHp-6): peroxidase with high activity.
Ru-Promoted CO2 activation for oxidative dehydrogenation of propane over chromium oxide catalyst
Authors: Jin Renxi , Easa Justin , Tran Dat T. , O'Brien Casey P.
Published: February 2020

A volcano-shape propylene production rate trend as Ru loading on CrOx catalysts increases for CO2-assisted C3H8 dehydrogenation reaction.
Lewis acid sites in MOFs supports promoting the catalytic activity and selectivity for CO esterification to dimethyl carbonate
Authors: Xu Yu-Ping , Wang Zhi-Qiao , Tan Hong-Zi , Jing Kai-Qiang , Xu Zhong-Ning , Guo Guo-Cong
Published: February 2020

We studied the effect of Lewis acidity in metal–organic frameworks (MOFs) on their activity as catalyst supports for the esterification of CO to dimethyl carbonate.
Identification of bioactive compounds that contribute to the α-glucosidase inhibitory activity of rosemary
Authors: Ma Yan-Yan , Zhao Deng-Gao , Zhang Ruiqiang , He Xuan , Li Bao Qiong , Zhang Xiang-Zhi , Wang Zijian , Zhang Kun
Published: February 2020

To investigate the bioactive compounds that contribute to the α-glucosidase inhibitory activity of rosemary, phenolics and triterpene acids were characterized and quantified using quadrupole-Orbitrap mass spectrometry and enzyme assay.
Intestinal bioavailability of n-3 long-chain polyunsaturated fatty acids influenced by the supramolecular form of phospholipids
Authors: Sehl Anthony , Couëdelo Leslie , Vaysse Carole , Cansell Maud
Published: February 2020

This work aims at studying the bioavailability of n-3 long-chain polyunsaturated fatty acids carried by marine phospholipids, formulated in different supramolecular forms,i.e.oil-in-water emulsion and liposomes.
Synthesis and polymerisation of α-alkylidene cyclic carbonates from carbon dioxide, epoxides and the primary propargylic alcohol 1,4-butynediol
Authors: Dabral Saumya , Licht Ulrike , Rudolf Peter , Bollmann Gérard , Hashmi A. Stephen K. , Schaub Thomas
Published: February 2020

Using the bulk chemical 1,4-butynediol, readily available epoxides and carbon dioxide, a new series of unsubstituted exovinylene carbonates were synthesised.
A comprehensive experimental and theoretical study on BN nanosheets for the adsorption of pharmaceutical drugs
Authors: Goyal Ankita , Aggarwal Diksha , Kapoor Surbhi , Goel Neetu , Singhal Sonal , Shukla Jaya
Published: February 2020

BN nanosheets were explored as proficient adsorbents for pharmaceutical drugs owing to high adsorption capacity and favorable interactions.
CHF2CF2OCHF2: conformational analysis and direct dynamics study of its reaction with Cl atoms and atmospheric fate
Authors: Baidya Bidisha , Lily Makroni , Patgiri Dimpal , Hynniewta Shemphang , Chandra Asit K.
Published: February 2020

Conformers of CHF2CF2OCHF2 are identified, and the kinetics of its reaction with Cl atoms and final atmospheric degradation products are studied to assess atmospheric impact.
High-order structures from nucleic acids for biomedical applications
Authors: Hill Alyssa C. , Hall Jonathan
Published: February 2020

This article reviews important developments in DNA nanotechnology and RNA nanotechnology and highlights supramolecular nucleic acid structures with biomedical applications.
iTRAQ-based proteomic and physiological analyses of mustard sprouts in response to heat stress
Authors: Cheng Chao , Liu Yin , Fang Weiming , Tao Jun , Yang Zhengfei , Yin Yongqi
Published: February 2020

Heat stress has been proved to increase the content of melatonin in plants.
Fused heterocycle-based energetic materials (2012–2019)
Authors: Gao Haixiang , Zhang Qinghua , Shreeve Jean'ne M.
Published: February 2020

Fused heterocycles are unique building blocks for the synthesis of a wide range of high-performance energetic materials.
A “competitive occupancy” strategy toward Co–N4 single-atom catalysts embedded in 2D TiN/rGO sheets for highly efficient and stable aromatic nitroreduction
Authors: Gu Ying , Wu Aiping , Wang Lei , Wang Dongxu , Yan Haijing , Yu Peng , Xie Ying , Tian Chungui , Sun Fanfei , Fu Honggang
Published: February 2020

Single-atom M–N-based catalysts have been synthesized based on the “competitive occupancy” of Ti species and Co2+ (Ni2+etc.) on GO, and can be used as a highly active and stable hydrogenation catalyst.
Excitation-power responsive upconversion logic operations based on the multiphoton process of a praseodymium ion
Authors: Nie Jingheng , Gu Jianmin , Ying Weitao , Wang Fei , Xu Shiqing , Liu Shimin
Published: February 2020

Controlling the spectral regulation properties of Pr3+ion doped material can realize by the excitation power due to the unique multiphoton processes, which could be utilized to design elementary logic operations and integrative logic operations.
Atomic-dispersed platinum anchored on porous alumina sheets as an efficient catalyst for diboration of alkynes
Authors: Shang Huishan , Chen Wenxing , Jiang Zhuoli , Zhou Danni , Zhang Jiatao
Published: February 2020

An atomic-dispersed Pt catalyst (Pt/dp-Al2O3) was fabricated via a simple wet impregnation method and demonstrated to be highly active and stable for the diboration of phenylacetylene, which is due to Pt/dp-Al2O3 providing unique catalytic sites.
Elucidating the capacitive desalination behavior of NaxCoO2: the significance of electrochemical pre-activation
Authors: Liu Zhenzhen , Ma Wei , Li Haibo
Published: February 2020

This work explores the capacitive deionization behavior of NaxCoO2 and highlights the significance of electrochemical pre-activation in the desalination process.
Real-time detection of mRNA splicing variants with specifically designed reverse-transcription loop-mediated isothermal amplification
Authors: Su Fengxia , Wang Guanhao , Ji Jianing , Zhang Pengbo , Wang Fangfang , Li Zhengping
Published: February 2020

A novel splicing variant assay is developed based on specifically designed reverse-transcription (RT) loop-mediated isothermal amplification.
Selective radical depolymerization of cellulose to glucose induced by high frequency ultrasound
Authors: Haouache Somia , Karam Ayman , Chave Tony , Clarhaut Jonathan , Amaniampong Prince Nana , Garcia Fernandez José M. , De Oliveira Vigier Karine , Capron Isabelle , Jérôme François
Published: February 2020

Ultrasonic irradiation of cellulose at a high frequency induces its selective depolymerization to glucose at room temperature and atmospheric pressure within only a few minutes.
Research Mentoring: Decreasing the Gap in Clinical Application of Evidence in the School-Based Setting
Authors: Smith Janice C. , Ward McKenzie
Published: February 2020

Purpose

The purpose of this article was to describe how effective research mentoring, with emphasis on implementation science, may contribute to speech-language pathologists' experiences integrating current evidence into practice in the school-based setting during the clinical fellowship (CF) year. A case example will be used to illustrate mentoring outcomes and provide insight from a 1st year professional, currently in the CF year, who completed a research mentoring program. This case example will detail a faculty-graduate student research mentorship experience and the carryover influence on the new fellow's 1st year of clinical practice.

Conclusion

Research mentoring emphasizing implementation science methods and identified qualities of effective mentoring has the potential to contribute to speech-language pathologists' experiences integrating current evidence into practice in the school-based setting during the CF year. Certainly, outcomes of mentorship experiences are contingent on the quality of mentoring activities and the characteristics of both mentor and mentee. However, it is important to consider that the value of good mentoring may add to the transition from student to professional, particularly with regard to implementing research-supported strategies and techniques into daily practice.
Frequency reconfigurable antenna based on commercial graphene nanoplatelets
Authors: Yasir M. , Savi P.
Published: February 2020

A voltage‐controlled frequency reconfigurable antenna is presented. The reconfigurable antenna comprises of a microstrip patch antenna connected to two grounded stubs via commercial graphene nanoplatelets. An applied bias voltage varies the resistance of graphene. The length and width of the stubs are optimised to cause a change in reactance because of a change in graphene resistance. A change in the reactance at the radiating edge of the patch antenna varies its resonant frequency. Commercial graphene nanoplatelets bear higher sheet resistance. The complete structure is designed to provide maximum frequency shift with minimum mismatch. The optimised simulated design is fabricated and comparable measured results are obtained. The resulting measured frequency shift is 310 MHz at a frequency of 5 GHz.
Characterisation of suprathermal electron pitch-angle distributions
Authors: Carcaboso Fernando , Gómez-Herrero Raúl , Espinosa Lara Francisco , Hidalgo Miguel A. , Cernuda Ignacio , Rodríguez-Pacheco Javier
Published: February 2020

Context. Suprathermal electron pitch-angle distributions (PADs) contain substantial information about the magnetic topology of the solar wind. Their characterisation and quantification allow us to automatically identify periods showing certain characteristics.

Aims. This work presents a robust automatic method for the identification and statistical study of two different types of PADs: bidirectional suprathermal electrons (BDE, often associated with closed magnetic structures) and isotropic (likely corresponding to solar-detached magnetic field lines or highly scattered electrons).

Methods. Spherical harmonics were fitted to the observed suprathermal PADs of the 119–193 eV energy channel of STEREO/SWEA from March 2007 to July 2014, and they were characterised using signal processing analysis in order to identify periods of isotropic and bidirectional PADs. The characterisation has been validated by comparing the results obtained here with those of previous studies.

Results. Interplanetary coronal mass ejections (ICMEs) present longer BDE periods inside the magnetic obstacles. A significant amount of BDE remain after the end of the ICME. Isotropic PADs are found in the sheath of the ICMEs, and at the post-ICME region likely due to the erosion of the magnetic field lines. Both isotropy and BDE are solar-cycle dependent. The isotropy observed by STEREO shows a nearly annual periodicity, which requires further investigation. There is also a correspondence between the number of ICMEs observed and the percentage of time showing BDE.

Conclusions. A method to characterise PADs has been presented and applied to the automatic identification of two relevant distributions that are commonly observed in the solar wind, such as BDE and isotropy. Four catalogues (STEREO-A and STEREO-B for isotropic and BDE periods of at least 10 min) based on this identification are provided for future applications.
Automated process control system for closed water supply installations for fish cultivation
Authors: Safin M.A. , Gerasimov E.I.
Published: February 2020

The paper describes an automated process control unit for closed water supply system for yearround fish farming in closed aquaculture farms. The operation principles and the technological process for maintaining stable fish growth conditions are discussed.
ISSR Analysis on Genetic Diversity of Endangered Plant Parrotia subaequalis in Dalonggou of Yixing, Jiangsu
Authors: Yun-xia Chen , Jun-fan Jiang , Cheng-hui Nan , Xiao-ming Xue
Published: February 2020

In this study, 25 flower samples of Parrotia subaequalis individuals were collected in Dalonggou of Yixing, Jiangsu. ISSR markers were used to analyze the genetic diversity of the 25 individuals. Six primers were selected and used for PCR amplification. The result showed that 59 discernible DNA fragments were amplified, among them 47 are polymorphic loci and the percentage of polymorphic loci (PPB) is 79.7%. By analysis with POPGENE32, the effective number of alleles (Ne) is 1.4697, Nei's gene diversity (H) is 0.2792, Shannon's Information index (I) is 0.4195, the Ewens-Watterson Neutral value is between 0.02 to 1, and more than half of the neutral test values are above 0.5. Through cluster analysis with NTSYSpc-2.1, genetic similarity coefficients of 25 samples are between 0.25 and 1 with an average of 0.71. From the results of this study we can get a conclusion that the genetic differences among the wild populations of P. subaequalis in Dalonggou of Yixing, Jiangsu were large and rich in diversity. The aim of this study is to reveal the genetic diversity of wild population of P. subaequalis from molecular level and provide a theoretical basis for the protection of this rare and endangered plant.
Study on Optimization of fermentation technology of carambola enzyme
Authors: Chen Baihua , Liu Hua , Zheng Qiqi
Published: February 2020

in this paper, a new type of Carambola enzyme was developed from tropical fruit carambola. Through single factor experiment and orthogonal experiment, the mixed fermentation was carried out by using yeast and plant lactobacillus. The effects of the concentration of mixed bacteria, the proportion of mixed bacteria, the fermentation temperature and the fermentation time on the fermentation effect of Carambola enzyme were studied. The results showed that the best technological parameters of Carambola enzyme: feed liquid The ratio is 1:3, the sucrose content is 7%, the concentration of strain is 5%, the proportion of inoculated strain is 1:1, the optimal fermentation temperature is 30 ℃, and the fermentation time is 7 days. After verification, it has certain guiding significance for the development and industrial production of Carambola enzyme products.
Research of Plant Carbon Fixation and Oxygen Release
Authors: Li Peifeng , Liu Zhiqiang , Wang Dan , Zheng Jianhao , Wang Wei
Published: February 2020

Expressway service area is an important facility to provide various services for drivers and passengers on the expressway. With the rapid development of expressways in China and the continuous improvement of construction technology and operation level of service areas, more and more attention to the landscape design and plant ecological benefits of service areas. Based on the investigation of the capacity of carbon fixation and oxygen release of green plants in the service area of expressway, combined with the needs and characteristics of each functional area in the service area, this paper puts forward the basic principles and methods of low-carbon ecological landscape construction in the service area, studies the green plant combination mode in the service area, and improves the service level of green landscape and low-carbon ecology in the service area.
Research and Design of Wind and Solar Complementary Electric Sightseeing Boat
Authors: Zhang Haomin , Xu Xingang
Published: February 2020

To solve the problem of rapid development of domestic water tourism and water pollution in scenic waters, windsurfing technology and solar photovoltaic technology are applied to small and medium-sized sightseeing cruise ships. While optimizing the hull design and component coordination, the application of automatic control technology further improves the utilization efficiency of wind and light energy, and achieves the adaptation of the sail angle of attack and the efficient coupling of the two energies. The ship mainly uses the natural resources of solar energy and wind energy. It will not burn fossil fuels during the ship’s travel, thus reducing the emission of toxic and harmful substances, which has positive significance for environmental protection. In short, the solar wind-assisted power generation ship studied in this project has remarkable features such as zero emission, high degree of intelligence, significant wind-assisted effect, high efficiency of light energy utilization, and low hull space occupation. Compared with the ordinary lake area cruise ship, the energy-saving and emission reduction effect of the power generation ship is very obvious[1].
Viability of Najawa carp (Cyprinus carpio L.) sperm at 4°C temperature storage
Authors: Pradana Dimas Fendy , Hardaningsih Ignatius , Sari Dini Wahyu Kartika
Published: February 2020

The objectives of this study were to evaluate the sperm viability of Najawa carp (Cyprinus carpio L.) in cryopreservation pre-conditions at 4°C. The design used in this study was Complete Randomized Design with 4 treatments, BSS as a control, 10% DMSO, 0,2 M Sucrose, and 5% DMSO + 0,1 M Sucrose; each consist of three replications. The parameters observed were progressive motility of fresh sperm, diluted sperm before low temperature storage, and 2 hours; 3 hours; 4 hours; 5 hours; one day; one week; a month after 4°C storage. The data were analyzed by ANOVA. The data showed that there was no significant difference between treatment (P>0.05). The best viability was 40.56% of sperm motility which survive for one week, it was achieved by 5% DMSO + 0,1 M Sucrose.
Traditional Fisheries Supply Chain Management in The Border Area: The Natuna Islands, Indonesia
Authors: Wardono Budi , Muhartono Rizky , Apriliani Tenny , Hikmah  , Zulham Armen
Published: February 2020

Fisheries have an important role in the economic and security aspects of the border region. Fishing in Natuna is carried out by local fishermen using small vessels with simple fishing gear. The distribution system with ships was hampered due to the Natuna sea area factors and the limited number and frequency. The research aimed to find out the supply chain management of fish in the Natuna Island border area to meet local, regional and export needs. The research used a qualitative approach and snowball information. The result showed that market formation was based on the type and quality of fish caught. Production of local fishermen was sold to the Natuna market, regional markets (Pontianak and Tanjung Pinang), and export market to Singapore, Mainland China, Hongkong, and Malaysia. Type of fish with premium quality (grouper, red snapper) was exported and sold to restaurants, with oligopolistic market characteristics. The premium quality fish market share was controlled by a large trader (Tauke) who has links to islands far Natuna (Pulau Laut, Pulau Tiga, Midai and Serasan). Non-premium quality fish marketing was carried out by fishing boat owners or collectors for local consumption and regional markets.
Dynamics of Post-Fire Effects in Larch Forests of Central Siberia Based on Satellite Data
Authors: Yakimov Nikita , Ponomarev Evgenii
Published: February 2020

The article represents the results of Terra, Aqua / MODIS, Landsat-8/OLI satellite data analysis for fire damaged plots in larch forests of Central Siberia. The analysis of averaged surface temperature (brightness temperature) and vegetation index (NDVI) was performed for post-fire circumstances. Estimates of the state and dynamics of fire-damaged vegetation cover were obtained on the basis of inter-seasonal variation of the NDVI index. It was found that post-fire dynamics of vegetation cover determines the surface temperature anomalies within the fire scar plots during at least five years after wildfire impact. It was instrumentally registered that the maximum excess of brightness temperature on post-fire areas can reach up to 11°C comparing to that of background areas under the same conditions. Such anomalies are determined by higher level of insolation due to partial or total tree mortality, as well as by decreasing of on-ground cover thickness after fire impact on grass and moss-lichen covers. During the first year after a fire in larch forests of Siberia, the maximum temperature anomalies of the underlying surface was recorded in the third decade of June. In the course of 2—5 years after burning, the maximum temperature anomalies shift to the second or even third decade of July within the phenological season. The suggested approach allows to assess the degree of fire impact on vegetation, as well as to predict changes in the active layer of permafrost soils, which may be a consequence of extra thermal flow at the surface in the circumstances of disturbed larch forests of Siberia.
Comparative Analysis of Solar Panel Output Power: Matrix Vs Tree Form
Authors: Deep Rahul , Mishra Abhishek , Agarwal Anshul
Published: February 2020

Solar energy is most important form of renewable energy. It is cheap and easily available but it required a lot of area for production of solar power to install them in a particular space. This paper proposes a comparison of solar panel in matrix form and a solar power tree in a particular area. A solar power tree requires very less place to produce efficient energy. Solar Tree is made of metal structure , it has a number of solar panels which are used to give the shape of a tree and arranged in a tall tower. Spiralling phyllataxy is technique used to improve the efficiency of the plant as it prevent the lower panel shadowing. Solar photovoltaic modules are arranged in series form like fibonacci series in place of leaves in solar tree. By using this technique the amount of energy produced by solar tree is more than the energy produce by an array of solar cells. Solar trees are produces about 50% more electricity .The increasing energy demand, economy of land, the solar tree concept is very successful one and should be implemented to provide electricity. Solar tree is much better than the traditional solar PV system in area point of view and also more efficient.
Impact of insulation thicknesses of several types of thermal insulator on energy cost with respect to different climate zones in Morocco
Authors: Gounni Ayoub , Mabrouk Mohamed Tahar , Kheiri Abdelhamid , El Alami Mustapha
Published: February 2020

In Morocco the thermal insulation of buildings envelop was not a common practice until it becomes obligatory since 2015 to meet the requirements of the Moroccan construction thermal regulation (RTCM) depending on six climate zones. The aim of this paper is to determine the optimum thickness of expanded polystyrene (EPS) and rock wool (RW) of walls constructed of brick for different Moroccan climate zones: Agadir (first zone), Tanger (second zone), Fes (third zone), Ifrane (fourth zone), Marrakech (fifth zone), Errachidia (sixth zone). A numerical model of a multilayered wall is developed to compute the annual heating and cooling loads. These loads are used as input to life cycle cost analysis using the energy and insulation costs. The liquefied petroleum gas (LPG) and electricity are used as energy source, respectively, for heating and cooling. For each case, the calculation is carried out for annual heating and cooling loads and total cost including insulation and energy costs. Results show that the optimum thermal insulation depends on climate zones and insulation types. The lowest value of energy savings is obtained for climate zone 1 which are 289.55 and 300.55 dh/m² respectively for EPS and RW.
Selection Methodology of Potential Sensible Thermal Energy Storage Materials for Medium Temperature Applications
Authors: Hrifech Soukaina , Agalit Hassan , Bennouna El Ghali , Mimet Abdelaziz
Published: February 2020

Thermal energy storage (TES) component improves the revenue of a concentrating solar power (CSP) plant by allowing more heat to be stored and making the electric energy available during the absence of sunlight. The heat can be stored in three ways (sensible, latent, or thermochemical). The present work aims to identify and select cost-effective sensible TES systems suitable for the medium temperature range (100-300 °C) applications (e.g. Fresnel CSP plants, industrial waste heat recovery, etc.). Based on a literature review, a selection methodology is developed to select potential candidate solid TES media (e.g. natural rock, concrete, sand, etc. ) as filler material in direct or indirect contact with thermal oil, which is used generally as heat transfer fluid (HTF) for this temperature range. The main criteria and steps of this selection methodology are identified and they take into account the different decisive storage properties as thermo-physical and mechanical properties of the solid media. Finally, the potential candidate TES materials are identified for the targeted application and further indoor experimental investigations are briefly presented.
Cafeteria von Patienten für Patienten
Authors: Hahl Doris , Weisbrod Sabine
Published: February 2020

Patienten, die längerfristig in der Forensik untergebracht sind, zu motivieren, an therapeutischen Maßnahmen aktiv mitzuwirken, ist häufig schwierig. Die Vitos Klinik für forensische Psychiatrie im hessischen Riedstadt hat deshalb eine Cafeteria eingerichtet, die von Patienten für Patienten betrieben wird und eine willkommene Abwechslung zum Stationsalltag ist. Anhand von wirklichkeitsnahen Bedingungen lernen die Patienten eine reale Arbeitssituation kennen und wechseln von der Rolle des „zu Behandelnden“ in die Rolle des „Handelnden“.
Hilflosigkeit der Patientin
Authors: Otten Elena
Published: February 2020

Im 5. Teil unserer Serie geht es um eine 51-jährige Patientin, die seit ihrem Jugendalter unter starken Zwängen leidet. Seit dem Tod ihrer Mutter vor über 20 Jahren ist sie alkoholabhängig und depressiv. Sie hat das Gefühl, nichts zu können und anderen eine Last zu sein. Lesen Sie, wie das Pflegeteam es schafft, mithilfe der systemischen Fallbesprechung neue Perspektiven für sich und die Patientin zu entwickeln.
Traumasensible psychiatrische Pflege
Authors: Maria Reichel Anja
Published: February 2020

Traumatische Erfahrungen, wie körperliche oder sexuelle Gewalt, aber auch Krieg und Unfälle, können dazu beitragen, dass sich eine Posttraumatische Belastungsstörung entwickelt. Um traumatisierte Menschen auf ihrem Genesungsweg begleiten zu können, müssen Pflegende wissen, was die Betroffenen brauchen. Unsere Autorin hat deshalb ein traumaspezifisches Behandlungskonzept entwickelt, das sich am Bedarf der Betroffenen orientiert und individuell zugeschnittene Hilfe ermöglicht.
Plant Endophytes and Epiphytes: Burgeoning Sources of Known and “Unknown” Cytotoxic and Antibiotic Agents?
Authors: Newman David J. , Cragg Gordon M.
Published: February 2020

In the last 20 or so years, the influence of endophytes and, quite recently, epiphytes of plants upon the compounds found in those plants, which were usually assumed to be phytochemicals produced by the plant for a variety of reasons, often as a defense against predators, is becoming more evident, in particular in the case of antitumor agents originally isolated from plant sources, though antibiotic agents might also be found, particularly from epiphytes. In this review, we started with the first report in 1993 of a taxol-producing endophyte and then expanded the compounds discussed to include camptothecin, the vinca alkaloids, podophyllotoxin, and homoharringtonine from endophytic microbes and then the realization that maytansine is not a plant secondary metabolite at all, and that even such a well-studied plant such as Arabidopsis thaliana has a vast repertoire of potential bioactive agents in its leaf epiphytic bacteria. We have taken data from a variety of
sources, including a reasonable history of these discoveries that were not given in recent papers by us, nor in other papers covering this topic. The sources included the Scopus database, but we also performed other searches using bibliographic tools, thus, the majority of the papers referenced are the originals, though we note some very recent papers that have built on previous results. We concluded with a discussion of the more modern techniques that can be utilized to “persuade” endophytes and epiphytes to switch on silent biosynthetic pathways and how current analytical techniques may aid in evaluating such programs. We also comment at times on some findings, particularly in the case of homoharringtonine, where there are repetitious data reports differing by a few years claiming the same endophyte as the producer.