bluuebunny/crossref_metadata_2025 huggingface dataset:
Airborne Dissemination of Bacteria (Enterococci, Staphylococci and Enterobacteriaceae) in a Modern Broiler Farm and Its Environment
Authors: Sanz Susana , Olarte Carmen , Hidalgo-Sanz Raquel , Ruiz-Ripa Laura , Fernández-Fernández Rosa , García-Vela Sara , Martínez-Álvarez Sandra , Torres Carmen
Published: June 2021

The role of the air as a vehicle of bacteria dissemination in the farming environment has been previously reported, but still scarcely studied. This study investigated the bacteria density/diversity of the inside and outside air and of litter samples at a broiler farm. Samples were collected considering two seasons, three outside air distances (50/100/150 m) and the four cardinal directions. Selective media was used for staphylococci, enterococci, and Enterobacteriaceae recovery. A high number of bacteria was detected in the litter (2.9 × 105–5.8 × 107 cfu/g) and in the inside air (>105 cfu/m3), but a low emission of bacteria was evidenced in the outside air (<6 cfu/m3). Moreover, the bacteria detected in the farm’s outside air decreased the further from the farm the sample was taken. A total of 544 isolates were identified by MALDI-TOF (146 from the litter, 142 from inside air and 256 from outside air). From these, 162 staphylococci (14 species; S. saprophyticus 40.7%), 176 Enterobacteriaceae (4 species; E. coli 66%) and 190 enterococci (4 species; E. hirae 83%) were detected. E. hirae was the predominant species, and identical PFGE clones were detected in inside and outside samples. The detection of identical DNA profiles in E. hirae isolates from inside and outside samples suggests the role of the air in bacterial dissemination from the inside of the broiler farm to the immediate environment.
Breeding and Maintenance of Immunodeficient Mouse Lines under SPF Conditions—A Call for Individualized Severity Analyses and Approval Procedures
Authors: Kammertoens Thomas , Jeuthe Sarah , Baranzke Heike , Klippert Antonina , Thöne-Reineke Christa
Published: June 2021

In the EU, the breeding of genetically modified laboratory animals is, by definition, an animal experiment if the offspring may experience pain, suffering, or harm. In order to determine the actual burden of genetically modified mice, established methods are available. However, the breeding of immunodeficient mice is considered an experiment requiring a permit, even if no pain, suffering or harm is observed under scientifically required defined hygienic housing conditions, as determined by established methods of severity assessment. This seems contradictory and leads to uncertainty among scientists. With this commentary, we would like to shed light on this topic from different perspectives and propose a solution in terms of individualized severity assessment and approval procedures.
Biovalorization of Lignocellulosic Materials for Xylitol Production by the Yeast Komagataella pastoris
Authors: Araújo Diana , Costa Tatiana , Freitas Filomena
Published: June 2021

The main goal of this study was to screen different lignocellulosic materials for their ability to support the cell growth of the yeast Komagataella pastoris and the production of xylitol. Several lignocellulosic materials, namely banana peels, brewer’s spent grains (BSGs), corncobs, grape pomace, grape stalks, and sawdust, were subjected to dilute acid hydrolysis to obtain sugar rich solutions that were tested as feedstocks for the cultivation of K. pastoris. Although the culture was able to grow in all the tested hydrolysates, a higher biomass concentration was obtained for banana peels (15.18 ± 0.33 g/L) and grape stalks (14.58 ± 0.19 g/L), while the highest xylitol production (1.51 ± 0.07 g/L) was reached for the BSG hydrolysate with a xylitol yield of 0.66 ± 0.39 g/g. Cell growth and xylitol production from BSG were improved by detoxifying the hydrolysate using activated charcoal, resulting in a fourfold increase of the biomass production, while xylitol production was improved to 3.97 ± 0.10 g/L. Moreover, concomitant with arabinose consumption, arabitol synthesis was noticed, reaching a maximum concentration of 0.82 ± 0.05 g/L with a yield on arabinose of 0.60 ± 0.11 g/g. These results demonstrate the feasibility of using lignocellulosic waste, especially BSG, as feedstock for the cultivation of K. pastoris and the coproduction of xylitol and arabitol. Additionally, it demonstrates the use of K. pastoris as a suitable microorganism to integrate a zero-waste biorefinery, transforming lignocellulosic waste into two high-value specialty chemicals with high market demand.
A Novel Sucrose Isomerase Producing Isomaltulose from Raoultella terrigena
Authors: Liu Li , Yu Shuhuai , Zhao Wei
Published: June 2021

Isomaltulose is widely used in the food industry as a substitute for sucrose owing to its good processing characteristics and physicochemical properties, which is usually synthesized by sucrose isomerase (SIase) with sucrose as substrate. In this study, a gene pal-2 from Raoultella terrigena was predicted to produce SIase, which was subcloned into pET-28a (+) and transformed to the E. coli system. The purified recombinant SIase Pal-2 was characterized in detail. The enzyme is a monomeric protein with a molecular weight of approximately 70 kDa, showing an optimal temperature of 40 °C and optimal pH value of 5.5. The Michaelis constant (Km) and maximum reaction rate (Vmax) are 62.9 mmol/L and 286.4 U/mg, respectively. The conversion rate of isomaltulose reached the maximum of 81.7% after 6 h with 400 g/L sucrose as the substrate and 25 U/mg sucrose of SIase. Moreover, eight site-directed variants were designed and generated. Compared with the wild-type enzyme, the enzyme activities of two mutants N498P and Q275R were increased by 89.2% and 42.2%, respectively, and the isomaltulose conversion rates of three mutants (Y246L, H287R, and H481P) were improved to 89.1%, 90.7%, and 92.4%, respectively. The work identified a novel SIase from the Raoultella genus and its mutants showed a potential to be used for the production of isomaltulose in the industry.
Quantitative Evaluation of Unfilled Grout in Tendons of Prestressed Concrete Girder Bridges by Portable 950 keV/3.95 MeV X-ray Sources
Authors: Uesaka Mitsuru , Yang Jian , Dobashi Katsuhiro , Kusano Joichi , Mitsuya Yuki , Iizuka Yoshiyuki
Published: June 2021

We have developed porTable 950 keV/3.95 MeV X-band (9.3 GHz) electron linear accelerator (LINAC)-based X-ray sources and conducted onsite prestressed concrete (PC) bridge inspection in the last 10 years. A T-shaped PC girder bridge with a thickness of 200–400 mm and a box-shaped PC girder bridge with a thickness of 200–800 mm were tested. X-ray transmission images of flaws such as thinning, fray, and disconnection caused by corrosion of PC wires and unfilled grout were observed. A three-dimensional structural analysis was performed to estimate the reduction in the yield stress of the bridge. In this study, we attempted to evaluate the unfilled grout quantitatively because it is the main flaw that results in water filling and corrosion. In the measured X-ray images, we obtained gray values, which correspond to the X-ray attenuation coefficients of filled/unfilled grouts, PC wires (steel) in a sheath, and concrete. Then, we compared the ratio of the gray values of the filled/unfilled grouts and PC wires to determine the stage of the unfilled grout. We examined this quantitative evaluation using the data obtained from a real T-shaped PC girder bridge and model samples to simulate thick box-shaped PC girder bridges. We obtained a clear quantitative difference in the ratios for unfilled and filled grouts, which coincided with our visual perception. We synthesized the experience and data and proposed a quantitative analysis for evaluating the unfilled grout for subsequent steps such as structural analysis and destructive evaluation by boring surveys.
A Comprehensive Framework to Reinforce Evidence Synthesis Features in Cloud-Based Systematic Review Tools
Authors: Person Tatiana , Ruiz-Rube Iván , Mota José Miguel , Cobo Manuel Jesús , Tselykh Alexey , Dodero Juan Manuel
Published: June 2021

Systematic reviews are powerful methods used to determine the state-of-the-art in a given field from existing studies and literature. They are critical but time-consuming in research and decision making for various disciplines. When conducting a review, a large volume of data is usually generated from relevant studies. Computer-based tools are often used to manage such data and to support the systematic review process. This paper describes a comprehensive analysis to gather the required features of a systematic review tool, in order to support the complete evidence synthesis process. We propose a framework, elaborated by consulting experts in different knowledge areas, to evaluate significant features and thus reinforce existing tool capabilities. The framework will be used to enhance the currently available functionality of CloudSERA, a cloud-based systematic review tool focused on Computer Science, to implement evidence-based systematic review processes in other disciplines.
Phytoglycoproteins and Human Health: Current Knowledge and Future Applications
Authors: Okagu Innocent U. , Ohanenye Ikenna C. , Ezeorba Timothy P. C. , Udenigwe Chibuike C.
Published: June 2021

Over the years, humans have relied on plants as sources of nutrients and bioactive compounds that promote health and wellness. Interestingly, drug discovery has benefitted immensely from the use of bioactive phytochemicals derived from food and medicinal plants. Phytoglycoproteins (PGPs) are plant-derived proteins with sugar moieties covalently linked to amino acid side chains, formed by glycosylation during posttranslational modification of polypeptides. Several studies in the last two decades, including cell culture and animal studies, have documented a variety of health-beneficial effects of PGPs, including hypolipidemic, wound healing, antioxidant, anti-inflammatory, immunomodulatory, and anticancer properties. Despite the prospects, there is a dearth of information on the pharmacokinetics and toxicity of PGPs, including possible induction of immune reactions, and the potential effects of stereospecific variation in PGPs isomers on their physiological functions. Further exploration of the multifunctional glycoproteins will position them as strong candidates for the development of nutraceuticals and functional foods.
Kinect Azure–Based Accurate Measurement of Dynamic Valgus Position of the Knee—A Corrigible Predisposing Factor of Osteoarthritis
Authors: Uhlár Ádám , Ambrus Mira , Kékesi Márton , Fodor Eszter , Grand László , Szathmáry Gergely , Rácz Kristóf , Lacza Zsombor
Published: June 2021

(1) Dynamic knee valgus is a predisposing factor for anterior cruciate ligament rupture and osteoarthritis. The single-leg squat (SLS) test is a widely used movement pattern test in clinical practice that helps to assess the risk of lower-limb injury. We aimed to quantify the SLS test using a marker-less optical system. (2) Kinect validity and accuracy during SLS were established by marker-based OptiTrack and MVN Xsens motion capture systems. Then, 22 individuals with moderate knee symptoms during sports activities (Tegner > 4, Lysholm > 60) performed SLS, and this was recorded and analyzed with a Kinect Azure camera and the Dynaknee software. (3) An optical sensor coupled to an artificial-intelligence-based joint recognition algorithm gave a comparable result to traditional marker-based motion capture devices. The dynamic valgus sign quantified by the Q-angle at the lowest point of the squat is highly dependent on squat depth, which severely limits its comparability among subjects. In contrast, the medio-lateral shift of the knee midpoint at a fixed squat depth, expressed in the percentage of lower limb length, is more suitable to quantify dynamic valgus and compare values among individual patients. (4) The current study identified a new and reliable way of evaluating dynamic valgus of the knee joint by measuring the medial shift of the knee-over-foot at a standardized squat depth. Using a marker-less optical system widens the possibilities of evaluating lower limb functional instabilities for medical professionals.
A Scientometric-Based Review of Traffic Signal Control Methods and Experiments Based on Connected Vehicles and Floating Car Data (FCD)
Authors: Astarita Vittorio , Giofrè Vincenzo Pasquale , Guido Giuseppe , Vitale Alessandro
Published: June 2021

This paper reviews the state of the art in traffic signal control methods that are based on data coming from onboard smartphones or connected vehicles. The review of the state of the art is carried out by applying analytical scientometric tools (topic visualization, co-citation analysis to establish influential journals and references, country analysis based on coauthorship, trending-topics analysis carried out by overlay visualization). The introduction of autonomous and connected vehicles will allow city management organizations to introduce new intersection management systems that rely on real-time positional data coming from instrumented vehicles. Traditional vehicles also could benefit from these new technologies by profiting from better-regulated intersections. This paper using a scientometric approach frames all the scientific contributions aimed at the field of traffic signal methods and experiments based on connected vehicles and floating car data. The applied scientometric approach reveals trending ideas and concepts and identifies the relevant documents that can be consulted in order for scientists and professionals to develop further this field with the implementation of new traffic signal control systems that can “give the green light” to drivers.
Pathogens and Carcinogenesis: A Review
Authors: Hatta Muhammad Nur Adam , Mohamad Hanif Ezanee Azlina , Chin Siok-Fong , Neoh Hui-min
Published: June 2021

Cancer is a global health problem associated with genetics and unhealthy lifestyles. Increasingly, pathogenic infections have also been identified as contributors to human cancer initiation and progression. Most pathogens (bacteria, viruses, fungi, and parasites) associated with human cancers are categorized as Group I human carcinogens by the International Agency for Research on Cancer, IARC. These pathogens cause carcinogenesis via three known mechanisms: persistent infection that cause inflammation and DNA damage, initiation of oncogene expression, and immunosuppression activity of the host. In this review, we discuss the carcinogenesis mechanism of ten pathogens, their implications, and some future considerations for better management of the disease. The pathogens and cancers described are Helicobacter pylori (gastric cancer), Epstein-Barr virus (gastric cancer and lymphoma), Hepatitis B and C viruses (liver cancer), Aspergillus spp. (liver cancer), Opisthorchis viverrine (bile duct cancer), Clonorchis sinensis (bile duct cancer), Fusobacterium nucleatum (colorectal cancer), Schistosoma haematobium (bladder cancer); Human Papillomavirus (cervical cancer), and Kaposi’s Sarcoma Herpes Virus (Kaposi’s sarcoma).
Overcoming Tribal Boundaries: The Biocultural Heritage of Foraging and Cooking Wild Vegetables among Four Pathan Groups in the Gadoon Valley, NW Pakistan
Authors: Khan Sheharyar , Hussain Wahid , Sulaiman  , Shah Sikandar , Hussain Hidayat , Altyar Ahmed E. , Ashour Mohamed L. , Pieroni Andrea
Published: June 2021

The foraging and consumption of wild food plants is a long-standing tradition in many parts of the world and their importance in promoting food security has become more widely debated in recent years. The current study aimed to document, analyze, and interpret the traditional knowledge of non-cultivated vegetables among four Pathan tribes (Alisher Khel, Hadarzai, Haji Khel, and Umarzai) living in the Gadoon Valley, Swabi District, Khyber Pakhtunkhwa, NW Pakistan, and to evaluate how these practices vary among the considered tribal communities. A total of 104 informants were interviewed via a semi-structured, open-ended questionnaire and group discussions. The field survey was conducted from October 2018 to November 2020. Information about local names, growth habit, used plant parts, food/cooking details, medicinal perceptions, availability season, and market prices were collected. The field survey recorded 51 non-cultivated vegetables belonging to 24 botanical families, for which the frequently used plant parts included young leaves, stems, and flowers. The greatest number of use reports was recorded for Colocasia and the highest cultural index value was recorded for Rumex dentatus; the dominant botanical families were Asteraceae and Fabaceae (six species each). Seven species were found to be sold at local and regional markets. Cross-cultural analysis among the four considered tribes showed that the largest number of species was reported by members of the Hadarzai and Umarzai tribes, although most of the quoted wild vegetables were homogenously gathered among all considered communities, with some more idiosyncratic plant uses among the Umarzai group, who have likely been less affected by the erosion of traditional knowledge or possibly have had less access to traded cultivated vegetables. The novelty of the data was assessed by comparing it with the previously published wild food ethnobotanical literature of Pakistan, which showed fifteen new wild vegetables not yet reported in the NW of the country. The recorded food biocultural heritage should be seriously considered in future local development projects aimed at fostering environmental sustainability and food security.
Clocks, Rhythms, Sex, and Hearts: How Disrupted Circadian Rhythms, Time-of-Day, and Sex Influence Cardiovascular Health
Authors: Meléndez-Fernández O. Hecmarie , Walton James C. , DeVries A. Courtney , Nelson Randy J.
Published: June 2021

Cardiovascular diseases are the top cause of mortality in the United States, and ischemic heart disease accounts for 16% of all deaths around the world. Modifiable risk factors such as diet and exercise have often been primary targets in addressing these conditions. However, mounting evidence suggests that environmental factors that disrupt physiological rhythms might contribute to the development of these diseases, as well as contribute to increasing other risk factors that are typically associated with cardiovascular disease. Exposure to light at night, transmeridian travel, and social jetlag disrupt endogenous circadian rhythms, which, in turn, alter carefully orchestrated bodily functioning, and elevate the risk of disease and injury. Research into how disrupted circadian rhythms affect physiology and behavior has begun to reveal the intricacies of how seemingly innocuous environmental and social factors have dramatic consequences on mammalian physiology and behavior. Despite the new focus on the importance of circadian rhythms, and how disrupted circadian rhythms contribute to cardiovascular diseases, many questions in this field remain unanswered. Further, neither time-of-day nor sex as a biological variable have been consistently and thoroughly taken into account in previous studies of circadian rhythm disruption and cardiovascular disease. In this review, we will first discuss biological rhythms and the master temporal regulator that controls these rhythms, focusing on the cardiovascular system, its rhythms, and the pathology associated with its disruption, while emphasizing the importance of the time-of-day as a variable that directly affects outcomes in controlled studies, and how temporal data will inform clinical practice and influence personalized medicine. Finally, we will discuss evidence supporting the existence of sex differences in cardiovascular function and outcomes following an injury, and highlight the need for consistent inclusion of both sexes in studies that aim to understand cardiovascular function and improve cardiovascular health.
Ethanolic Extracts of Adlay Testa and Hull and Their Active Biomolecules Exert Relaxing Effect on Uterine Muscle Contraction through Blocking Extracellular Calcium Influx in Ex Vivo and In Vivo Studies
Authors: Huang Yun-Ju , Chen Yu-Chieh , Chen Hsin-Yuan , Chiang Yi-Fen , Ali Mohamed , Chiang Wenchang , Chung Cheng-Pei , Hsia Shih-Min
Published: June 2021

Dysmenorrhea is one of the most prevalent disorders in gynecology. Historically, adlay (Coix lachryma-jobi L. var. Ma-yuen Stapf.) has been explored for its anti-tumor, pain relief, anti-inflammatory, and analgesic effects. The aim of this study was to evaluate the effects of adlay seeds on the inhibition of uterine contraction and thus dysmenorrhea relief, in vitro and in vivo. HPLC-MS and GC were used to elucidate the ethyl acetate fraction of adlay testa ethanolic extract (ATE-EA) and ethyl acetate fraction of adlay hull ethanolic extract (AHE-EA). Elucidation yielded flavonoids, phytosterols, and fatty acids. Uterine leiomyomas and normal adjacent myometrial tissue were evaluated by oxytocin- and PG-induced uterine contractility. ATE-EA and AHE-EA suppressed uterine contraction induced by prostaglandin F2 alpha (PGF2α), oxytocin, carbachol, and high-KCl solution ex vivo. In addition, the external calcium (Ca2+) influx induced contraction, and increased Ca2+ concentration was inhibited by ATE-EA and AHE-EA on the uterine smooth muscle of rats. Furthermore, ATE-EA and AHE-EA effectively attenuated the contraction of normal human myometrium tissues more than adjacent uterine leiomyoma in response to PGF2α. 3,5,6,7,8,3′,4′-Heptamethoxyflavone and chrysoeriol produced a remarkable inhibition with values of IC50 = 24.91 and 25.59 µM, respectively. The experimental results showed that treatment with ATE-EA at 30 mg/day effectively decreased the writhing frequency both on the oxytocin-induced writhing test and acetic acid writhing test of the ICR mouse.
Architectural Evaluation of 3D-Printed Buildings
Authors: García-Alvarado Rodrigo , Moroni-Orellana Ginnia , Banda-Pérez Pablo
Published: June 2021

3D-printed constructions express the capacity of automated technologies to elaborate buildings through additive manufacturing. These constructions require an architectural design according to their manufacturing conditions. This research reviews buildings that have been executed with these technologies in order to determine architectural characteristics. From an Internet search, a register was compiled of 112 cases of 3D-printed constructions around the globe. They include some 10,000 m2 of built surface area and were mainly erected in the last five years. The review shows that cases were built by approximately thirty executors, mostly entrepreneurs’ companies who have made different buildings as single-family dwellings constructed with gantries or robotic arms, either on-site or in factories. Most of the components printed are walls, with single or double filaments with interior voids. In some cases, they integrate reinforcements and openings. The geometry of the 3D-printed constructions varies between orthogonal layouts that replicate existing buildings and spherical shapes that reflect printing capabilities. Many of the cases are a combination of these characteristics, mixing a technological adaptation to existing architecture and new operative conditions. This review reveals the emerging development of this construction system with the progressive consolidation of some architectural attributes.
Cancer Cells Shuttle Extracellular Vesicles Containing Oncogenic Mutant p53 Proteins to the Tumor Microenvironment
Authors: Bhatta Bibek , Luz Ishai , Krueger Christian , Teo Fanny Xueting , Lane David P. , Sabapathy Kanaga , Cooks Tomer
Published: June 2021

Extracellular vesicles (EVs) shed by cancer cells play a major role in mediating the transfer of molecular information by reprogramming the tumor microenvironment (TME). TP53 (encoding the p53 protein) is the most mutated gene across many cancer types. Mutations in TP53 not only result in the loss of its tumor-suppressive properties but also results in the acquisition of novel gain-of-functions (GOF) that promote the growth of cancer cells. Here, we demonstrate that GOF mutant p53 proteins can be transferred via EVs to neighboring cancer cells and to macrophages, thus modulating them to release tumor supportive cytokines. Our data from pancreatic, lung, and colon carcinoma cell lines demonstrate that the mutant p53 protein can be selectively sorted into EVs. More specifically, mutant p53 proteins in EVs can be taken up by neighboring cells and mutant p53 expression is found in non-tumor cells in both human cancers and in non-human tissues in human xenografts. Our findings shed light on the intricate methods in which specific GOF p53 mutants can promote oncogenic mechanisms by reprogramming and then recruiting non-cancerous elements for tumor progression.
Zinc Oxide Nanoparticles Can Intervene in Radiation-Induced Senescence and Eradicate Residual Tumor Cells
Authors: Wiesmann Nadine , Gieringer Rita , Viel Melanie , Eckrich Jonas , Tremel Wolfgang , Brieger Juergen
Published: June 2021

Despite recent advancements in tumor therapy, metastasis and tumor relapse remain major complications hindering the complete recovery of many cancer patients. Dormant tumor cells, which reside in the body, possess the ability to re-enter the cell cycle after therapy. This phenomenon has been attributed to therapy-induced senescence. We show that these cells could be targeted by the use of zinc oxide nanoparticles (ZnO NPs). In the present study, the properties of tumor cells after survival of 16 Gy gamma-irradiation were investigated in detail. Analysis of morphological features, proliferation, cell cycle distribution, and protein expression revealed classical hallmarks of senescent cells among the remnant cell mass after irradiation. The observed radiation-induced senescence was associated with the increased ability to withstand further irradiation. Additionally, tumor cells were able to re-enter the cell cycle and proliferate again after weeks. Treatment with ZnO NPs was evaluated as a therapeutical approach to target senescent cells. ZnO NPs were suitable to induce cell death in senescent, irradiation-resistant tumor cells. Our findings underline the pathophysiological relevance of remnant tumor cells that survived first-line radiotherapy. Additionally, we highlight the therapeutic potential of ZnO NPs for targeting senescent tumor cells.
Photodynamic Therapy: Targeting Cancer Biomarkers for the Treatment of Cancers
Authors: Wang Xinning , Luo Dong , Basilion James P.
Published: June 2021

Photodynamic therapy (PDT) is a well-documented therapy that has emerged as an effective treatment modality of cancers. PDT utilizes harmless light to activate non- or minimally toxic photosensitizers to generate cytotoxic species for malignant cell eradication. Compared with conventional chemotherapy and radiotherapy, PDT is appealing by virtue of the minimal invasiveness, its safety, as well as its selectivity, and the fact that it can induce an immune response. Although local illumination of the cancer lesions renders intrinsic selectivity of PDT, most photosensitizers used in PDT do not display significant tumor tissue selectivity. There is a need for targeted delivery of photosensitizers. The molecular identification of cancer antigens has opened new possibilities for the development of effective targeted therapy for cancer patients. This review provides a brief overview of recent achievements of targeted delivery of photosensitizers to cancer cells by targeting well-established cancer biomarkers. Overall, targeted PDT offers enhanced intracellular accumulation of the photosensitizer, leading to improved PDT efficacy and reduced toxicity to normal tissues.
Next Generation Cytogenetics in Myeloid Hematological Neoplasms: Detection of CNVs and Translocations
Authors: Chicano María , Carbonell Diego , Suárez-González Julia , Lois Sergio , Ballesteros-Culebras Mercedes , Andrés-Zayas Cristina , Muñiz Paula , Rodríguez-Macias Gabriela , Bastos-Oreiro Mariana , Font Patricia , Ballesteros Mónica , Kwon Mi , Anguita Javier , Díez-Martín José Luis , Buño Ismael , Martínez-Laperche Carolina
Published: June 2021

Conventional cytogenetics are the gold standard for the identification of chromosomal alterations recurrent in myeloid neoplasms. Some next-generation sequencing (NGS) panels are designed for the detection of copy number variations (CNV) or translocations; however, their use is far from being widespread. Here we report on the results of a commercial panel including frequent mutations, CNVs and translocations in myeloid neoplasms. Frequent chromosomal alterations were analyzed by NGS in 135 patients with myeloid neoplasms and three with acute lymphoblastic leukemia. NGS analysis was performed using the enrichment-capture Myeloid Neoplasm-GeneSGKit (Sistemas Genómicos, Spain) gene panel including 35 genes for mutational analysis and frequent CNVs and translocations. NGS results were validated with cytogenetics and/or MLPA when possible. A total of 66 frequent alterations included in NGS panel were detected, 48 of them detected by NGS and cytogenetics. Ten of them were observed only by cytogenetics (mainly trisomy 8), and another eight only by NGS (mainly deletion of 12p). Aside from this, 38 secondary CNVs were detected in any of the genes included mainly for mutational analysis. NGS represents a reliable complementary source of information for the analysis of CNVs and translocations. Moreover, NGS could be a useful tool for the detection of alterations not observed by conventional cytogenetics.
Synergistic Effect of Alkali Na and K Promoter on Fe-Co-Cu-Al Catalysts for CO2 Hydrogenation to Light Hydrocarbons
Authors: Zheng Yuhao , Xu Chenghua , Zhang Xia , Wu Qiong , Liu Jie
Published: June 2021

Alkali metal K- and/or Na-promoted FeCoCuAl catalysts were synthesized by precipitation and impregnation, and their physicochemical and catalytic performance for CO2 hydrogenation to light hydrocarbons was also investigated in the present work. The results indicate that Na and/or K introduction leads to the formation of active phase metallic Fe and Fe-Co crystals in the order Na < K < K-Na. The simultaneous introduction of Na and K causes a synergistic effect on increasing the basicity and electron-rich property, promoting the formation of active sites Fe@Cu and Fe-Co@Cu with Cu0 as a crystal core. These effects are advantageous to H2 dissociative adsorption and CO2 activation, giving a high CO2 conversion with hydrogenation. Moreover, electron-rich Fe@Cu (110) and Fe-Co@Cu (200) provide active centers for further H2 dissociative adsorption and O-C-Fe intermediate formation after adsorption of CO produced by RWGS. It is beneficial for carbon chain growth in C2+ hydrocarbons, including olefins and alkanes. FeCoCuAl simultaneously modified by K-Na exhibits the highest CO2 conversion and C2+ selectivity of 52.87 mol% and 89.70 mol%, respectively.
The PD-L1/PD-1 Axis Blocks Neutrophil Cytotoxicity in Cancer
Authors: Yajuk Olga , Baron Maya , Toker Sapir , Zelter Tamir , Fainsod-Levi Tanya , Granot Zvi
Published: June 2021

The PD-L1/PD-1 axis mediates immune tolerance and promotes tumor growth and progression via the inhibition of anti-tumor immunity. Blocking the interaction between PD-L1 and PD-1 was clinically shown to be beneficial in maintaining the anti-tumor functions of the adaptive immune system. Still, the consequences of blocking the PD-L1/PD-1 axis on innate immune responses remain largely unexplored. In this context, neutrophils were shown to consist of distinct subpopulations, which possess either pro- or anti-tumor properties. PD-L1-expressing neutrophils are considered pro-tumor as they are able to suppress cytotoxic T cells and are propagated with disease progression. That said, we found that PD-L1 expression is not limited to tumor promoting neutrophils, but is also evident in anti-tumor neutrophils. We show that neutrophil cytotoxicity is effectively and efficiently blocked by tumor cell-expressed PD-1. Furthermore, the blocking of either neutrophil PD-L1 or tumor cell PD-1 maintains neutrophil cytotoxicity. Importantly, we show that tumor cell PD-1 blocks neutrophil cytotoxicity and promotes tumor growth via a mechanism independent of adaptive immunity. Taken together, these findings highlight the therapeutic potential of enhancing anti-tumor innate immune responses via blocking of the PD-L1/PD-1 axis.
Measuring the Anisotropy in Interfacial Tension of Nematic Liquid Crystals
Authors: Honaker Lawrence , Sharma Anjali , Schanen Andy , Lagerwall Jan
Published: June 2021

Liquid crystal (LC) phases typically show anisotropic alignment-dependent properties, such as viscosity and dielectric permittivity, so it stands to reason that LCs also have anisotropic interfacial tensions. Measuring the interfacial tension γ of an LC with conventional methods, such as pendant drops, can be challenging, however, especially when we need to know γ for different LC aligning conditions, as is the case when we seek Δγ, the interfacial tension anisotropy. Here, we present measurements of Δγ of the common synthetic nematic LC compound 5CB against water using a microfluidic droplet aspiration technique. To ensure tangential and normal alignment, respectively, we add poly(vinyl alcohol) (PVA) and sodium dodecylsulfate (SDS), respectively, as a stabilizer and measure γ for different concentrations of stabilizer. By fitting the Szyszkowski equation to the data, we can extrapolate to zero-stabilizer concentration, obtaining the γ of 5CB to pure water for each alignment. For normal alignment, we find γ⊥=31.9±0.8 mN·m−1, on the order of 1 mN·m−1 greater than γ||=30.8±5 mN·m−1 for tangential alignment. This resonates with the empirical knowledge that 5CB aligns tangentially to an interface with pure water. The main uncertainty arises from the use of polymeric PVA as tangential-promoting stabilizer. Future improvements in accuracy may be expected if PVA can be replaced by a low molar mass stabilizer that ensures tangential alignment.
uPAR PET/CT for Prognostication and Response Assessment in Patients with Metastatic Castration-Resistant Prostate Cancer Undergoing Radium-223 Therapy: A Prospective Phase II Study
Authors: Fosbøl Marie Øbro , Mortensen Jann , Petersen Peter Meidahl , Loft Annika , Madsen Jacob , Kjaer Andreas
Published: June 2021

The aim of this Phase II study was to investigate the potential for response assessment and prognostication of positron emission tomography (PET) using the ligand 68Ga-NOTA-AE105 targeting the urokinase-type plasminogen activator receptor (uPAR) in patients receiving Radium-223-dichloride therapy (223RaCl2). A combined whole-body uPAR PET and computed tomography (CT) was performed before initiation of 223RaCl2 and after two cycles of therapy. Standardized uptake value (SUV) in selected bone metastases was measured and the lesion with the highest SUVmax was considered the index lesion. Clinical outcomes were overall survival (OS), radiographic progression free survival (rPFS) and occurrence of symptomatic skeletal event (SSE). A total of 17 patients were included and 14 patients completed both baseline and follow-up uPAR-PET/CT. Baseline SUVmax of the index lesion was associated with OS; hazard ratio 2.51 (95% CI: 1.01–6.28, p = 0.05) per unit increase in SUVmax. No association between changes in SUVmax from baseline to follow-up and OS, progression during therapy, or rPFS was found. Baseline SUVmax was a significant predictor of SSE with receiver operating characteristics (ROC) area under the curve (AUC) = 0.81 (95% CI: 0.58–1.00, p = 0.034). A cut-off for tumor SUVmax could be established with an odds ratio of 14.0 (95% CI: 1.14–172.6, p = 0.023) for occurrence of SSE within 12 months. Although based on a small number of patients, uPAR-PET SUVmax in bone metastases was predictive for OS and risk of SSE in mCRPC patients receiving 223RaCl2. However, a relatively low uptake of the uPAR ligand in bone metastases impedes visual evaluation and requires another modality for lesion delineation.
Evaluation of a Clinical Index as a Predictive Tool for Primary Ciliary Dyskinesia
Authors: Martinů Vendula , Bořek-Dohalská Lucie , Varényiová Žofia , Uhlík Jiří , Čapek Václav , Pohunek Petr , Koucký Václav
Published: June 2021

Background: In primary ciliary dyskinesia (PCD) there is no single diagnostic test. Different predictive tools have been proposed to guide referral of high-risk patients for further diagnostic workup. We aimed to test clinical index (CI) on a large unselected cohort and compare its characteristics with other widely used tools—PICADAR and NA-CDCF. Methods: CI, PICADAR, and NA-CDCF scores were calculated in 1401 patients with suspected PCD referred to our center. Their predictive characteristics were analyzed using receiver operating characteristics (ROC) curves and compared to each other. Nasal nitric oxide (nNO) was measured in 569 patients older than 3 years. Results: PCD was diagnosed in 67 (4.8%) patients. CI, PICADAR, and NA-CDCF scores were higher in PCD than in nonPCD group (all p < 0.001). The area under the ROC curve (AUC) for CI was larger than for NA-CDCF (p = 0.005); AUCPICADAR and AUCNA-CDCF did not differ (p = 0.093). An overlap in signs and symptoms among tools was identified. PICADAR could not be assessed in 86 (6.1%) patients without chronic wet cough. For CI laterality or congenital heart defects assessment was not necessary. nNO further improved predictive power of all three tools. Conclusion: CI is a feasible predictive tool for PCD that may outperform PICADAR and NA-CFCD.
Genetic Algorithms to Maximize the Relevant Mutual Information in Communication Receivers
Authors: Lewandowsky Jan , Dongare Sumedh Jitendra , Martín Lima Rocío , Adrat Marc , Schrammen Matthias , Jax Peter
Published: June 2021

The preservation of relevant mutual information under compression is the fundamental challenge of the information bottleneck method. It has many applications in machine learning and in communications. The recent literature describes successful applications of this concept in quantized detection and channel decoding schemes. The focal idea is to build receiver algorithms intended to preserve the maximum possible amount of relevant information, despite very coarse quantization. The existent literature shows that the resulting quantized receiver algorithms can achieve performance very close to that of conventional high-precision systems. Moreover, all demanding signal processing operations get replaced with lookup operations in the considered system design. In this paper, we develop the idea of maximizing the preserved relevant information in communication receivers further by considering parametrized systems. Such systems can help overcome the need of lookup tables in cases where their huge sizes make them impractical. We propose to apply genetic algorithms which are inspired from the natural evolution of the species for the problem of parameter optimization. We exemplarily investigate receiver-sided channel output quantization and demodulation to illustrate the notable performance and the flexibility of the proposed concept.
Evaluation of a Filtering Facepiece Respirator and a Pleated Particulate Respirator in Filtering Ultrafine Particles and Submicron Particles in Welding and Asphalt Plant Work Environments
Authors: Mitra Aniruddha , Adhikari Atin , Martin Clinton , Dardano Gracia , Wagemaker Pascal , Adeoye Caleb
Published: June 2021

Manufacturing sites, such as welding, casting, and asphalt production (fumes), generate vast numbers of ultrafine particles of <0.1 µm in size and submicron particles close to the ultrafine range (0.1–0.5 µm). Although cumulative masses of these particles are negligible in comparison to the larger particles, the health effects are more severe due to the higher penetration in the human lower respiratory tract, other body parts crossing the respiratory epithelial layers, and the larger surface area. This research investigates the effectiveness of two common commercially available N95 filtering facepieces and N95 pleated particulate respirator models against ultrafine and submicron particles. Two specific types of respirators, the N95 filtering facepiece and the N95 pleated particulate models, in both sealed and unsealed conditions to the manikin face, were tested at various commercial and academic manufacturing sites, a welding and foundry site, and an asphalt production plant. Two TSI Nanoscan SMPS nanoparticle counters were used simultaneously to collect data for particles of 10–420 nm in size from inside and outside of the respirators. While one of them represented the workplace exposure levels, the other one accounted for the exposure upon filtration through the respiratory surfaces. The results showed the particles generated by these manufacturing operations were mostly within the range of from 40 to 200 nm. Results also indicated that while the percentage of filtration levels varied based on the particle size, it remained mostly within the desired protection level of 95% for both of the N95 respirator models in sealed conditions and even for the N95 pleated particulate model in the unsealed condition. However, in the case of the N95 filtering facepiece model, unsealed respirators showed that the percentage of penetration was very high, decreasing the protection levels to 60% in some cases. Although the number of workplace airborne particle levels varied considerably, the filtration percentages were relatively consistent.
Preliminary Study of Lead-Contaminated Drinking Water in Public Parks—An Assessment of Equity and Exposure Risks in Two Texas Communities
Authors: Fawkes Leanne , Sansom Garett
Published: June 2021

Safe drinking water is celebrated as a public health achievement and is a top priority for the Environmental Protection Agency. Yet today, lead (Pb) contaminated drinking water has the potential to be a public health crisis in the United States. Despite efforts to provide safe drinking water, update water infrastructure, and ensure strict drinking water regulations, there are incidents of unsafe lead levels and reports of associated adverse health effects. While there has been increased attention paid to the quality of drinking water within individuals’ homes, little research has examined the presence and concentration of lead in water from drinking fountain sources located in public parks. In this study, we sampled drinking water from every accessible public park in the Bryan/College Station (BCS), TX metropolitan area (N = 56). With a lower detection level of 2.0 μg/L, we discovered a mean lead concentration of 1.3 μg/L across all sites and a maximum of 8.0 μg/L. Furthermore, neighborhoods below the median income for BCS were twice as likely to have detectable lead levels in their water and had 1.5 times the mean concentration. This study underscores the need for action and supports previous studies that have identified a disparate burden to lead exposure among low socioeconomic populations within the United States. By examining the water quality in drinking fountains in publicly accessible parks, the results of our study provide public health professionals with important information about where infrastructure should be improved and the potential harms of lead in drinking fountain water.
Adaptation and Psychometric Properties of the Scale of Positive and Negative Experience (SPANE) in the General Colombian Population
Authors: Martín-Carbonell Marta , Checa Irene , Fernández-Daza Martha , Paternina Yadid , Espejo Begoña
Published: June 2021

(1) Background: Diener’s Scale of Positive and Negative Experiences (SPANE) assesses the presence and intensity of positive and negative affects, since these are considered basic aspects of the study of well-being. This article studies its psychometric properties in the general Colombian population. (2) We conducted a cross-sectional study of a sample of 1255 Colombians and we used structural equation modeling to confirm the bifactor structure. Additionally, we studied invariance by gender, and convergent and concurrent validity. (3) We found acceptable fit indicators for the bifactor model (CFI = 0.889, RMSEA = 0.046, SRMR = 0.059) as well as for the convergent (CFI = 0.909, RMSEA = 0.050, SRMR = 0.063) and concurrent (CFI = 0.966, RMSEA = 0.036, SRMR = 0.041) validity models. We did not confirm total invariance across gender, although we found configural and metric invariance, so percentiles by sex were provided. (4) Conclusions: The SPANE is a valid and reliable measure to assess well-being among the Colombian population, although we alert researchers to the risk of comparing affectivity average scores between sexes.
Developing an Ear Health Intervention for Rural Community Pharmacy: Application of the PRECEDE-PROCEED Model
Authors: Taylor Selina , Cairns Alice , Glass Beverley
Published: June 2021

Unaddressed hearing loss affects an estimated 466 million people worldwide, costing over $750 billion globally, with rural communities being particularly disadvantaged, due to the greater inequity in access to healthcare services. This mixed-methods study aimed to use the PRECEDE-PROCEED model to develop and pilot a rural community pharmacy-based ear health service, LISTEN UP (Locally Integrated Screening and Testing Ear aNd aUral Program). The PRECEDE process involved an assessment of the predisposing, reinforcing and enabling constructs to support practice change through a scoping review, stakeholder surveys and interviews and consultation with governing bodies and regulatory authorities. The PROCEED segment structured the evaluation of the service pilot and informed planned implementation, process, impact and outcome evaluation. The pilot study conducted in February 2021 included 20 participants, with the most common ear complaints presented being pain, pressure or blockage. All these participants reported high levels of satisfaction with the service, would recommend the service to others and would attend the pharmacy first before seeing a GP for future ear complaints. The PRECEDE-PROCEED model provides a comprehensive model to guide the design of the LISTEN UP program, an innovative model, expanding services offered by rural community pharmacies, with preliminary results demonstrating high consumer satisfaction.
Psychosocial and Diet-Related Lifestyle Clusters in Overweight and Obesity
Authors: Godoy-Izquierdo Débora , Lara Raquel , Ogallar Adelaida , Rodríguez-Tadeo Alejandra , Ramírez María J. , Navarrón Estefanía , Arbinaga Félix
Published: June 2021

This study explored intraindividual multidimensional profiles integrating psychosocial factors, namely, body image and satisfaction, weight-related self-stigma, positivity, and happiness, and behavioural-lifestyle factors, namely, adherence to a healthy diet, among Spanish adults with overweight or obesity. We further aimed to investigate the association of excess weight (i.e., measured body mass index, BMI) with the abovementioned multidimensional configurations. A convenience sample of 100 adult individuals (60% females) with excessive weight (69% overweight; 31% obesity) was recruited. They completed self-reports regarding the study variables, and their weight and height were measured. With a perspective centered on the individual, a cluster analysis was performed. Three distinct intraindividual psychosocial and diet-related profiles were identified: a group of healthy individuals with excess weight (46%); a group of individuals who were negatively affected by their excessive weight and showed the most distressed profile (18%); and a group of dysfunctional individuals who seemed to be excessively unrealistic and optimistic regarding their excessive weight and unhealthy lifestyles, but were troubled by their weight (36%). Furthermore, individuals in the affected cluster had higher obesity (mean BMI ± SD = 32.1 ± 3.7) than those in the clusters of healthy (28.0 ± 3.0) and dysfunctional individuals (28.1 ± 3.3) (p < 0.05). The results showed that there are specific psychosocial and lifestyle profiles in the adult population with excess weight and that there are relationships among psychological, behavioural, and body-composition factors. For clinical application purposes, it is important to account for the heterogeneity within individuals who are obese and to individualize the interventions, with a focus from weight change to the individual’s overall well-being.