bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Aflatoxin B1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy
Authors: Feng Yanting , He Lei , Wang Ling , Mo Rijian , Zhou Chunxia , Hong Pengzhi , Li Chengyong
Published: May 2020

An Aflatoxin B1 (AFB1) biosensor was fabricated via an Ag nanoparticles assembly on the surface of a porous anodized aluminum (PAA) membrane. First, the Raman reporter 4-Aminothiophenol (4-ATP) and DNA (partially complementary to AFB1 aptamer) were attached to the surface of Ag nanoparticles (AgNPs) by chemical bonding to form a 4-ATP-AgNPs-DNA complex. Similarly, the surface of a PAA membrane was functionalized with an AFB1 aptamer. Then, the PAA surface was functionalized with 4-ATP-AgNPs-DNA through base complementary pairing to form AgNPs-PAA sensor with a strong Raman signal. When AFB1 was added, AgNPs would be detached from the PAA surface because of the specific binding between AFB1 and the aptamer, resulting in a reduction in Raman signals. The detection limit of the proposed biosensor is 0.009 ng/mL in actual walnut and the linear range is 0.01–10 ng/mL. The sensor has good selectivity and repeatability; it can be applied to the rapid qualitative and quantitative detection of AFB1.
Antifungal Potential of Nanostructured Crystalline Copper and Its Oxide Forms
Authors: Oussou-Azo Auriane Fifame , Nakama Tomoki , Nakamura Masayuki , Futagami Taiki , Vestergaard Mun’delanji Catherine M.
Published: May 2020

Copper has been used as an antimicrobial agent for over a century and is now being added to commercial fungicides. Nanomaterials have attracted much attention due to the special properties they have over their bulk form. We studied nanostructured copper (Cu-NPs), investigating the potential for improved antifungal properties derived from its special properties and studied any effect that the oxidation of copper (CuO-NPs) may have. We conducted this research against Colletotrichum gloeoesporioides, a devastating pathogen to plants/crops worldwide. Research on the effects of copper on this fungus are limited. Our studies showed that nanoforms of copper had significant antifungal activities, with Cu-NPs offering the most sustainable efficacy and was more effective than its oxidative form (CuO-NPs). Scanning Electron Microscopy (SEM) images of the treated pathogen show that the hyphae had a swollen appearance, lost their filamentous structure, and the mycelia had a powder-like structure, indicating the probable destruction of the hyphal tubular cell wall. X-ray Difractogram (XRD) outputs showed substantial changes in the physical characteristics of the Cu-NPs after interaction with the fungus. This is the first report to demonstrate chemo-physical changes in the metal compounds, opening new insights for further studies on the mechanism of copper’s antifungal properties.
The Structural, Electronic, and Optical Properties of Ge/Si Quantum Wells: Lasing at a Wavelength of 1550 nm
Authors: Li Hongqiang , Wang Jianing , Bai Jinjun , Zhang Shanshan , Zhang Sai , Sun Yaqiang , Dou Qianzhi , Ding Mingjun , Wang Youxi , Qu Dan , Du Jilin , Tang Chunxiao , Li Enbang , Prades Joan Daniel
Published: May 2020

The realization of a fully integrated group IV electrically driven laser at room temperature is an essential issue to be solved. We introduced a novel group IV side-emitting laser at a wavelength of 1550 nm based on a 3-layer Ge/Si quantum well (QW). By designing this scheme, we showed that the structural, electronic, and optical properties are excited for lasing at 1550 nm. The preliminary results show that the device can produce a good light spot shape convenient for direct coupling with the waveguide and single-mode light emission. The laser luminous power can reach up to 2.32 mW at a wavelength of 1550 nm with a 300-mA current. Moreover, at room temperature (300 K), the laser can maintain maximum light power and an ideal wavelength (1550 nm). Thus, this study provides a novel approach to reliable, efficient electrically pumped silicon-based lasers.
Protein Supramolecular Structures: From Self-Assembly to Nanovaccine Design
Authors: Zottig Ximena , Côté-Cyr Mélanie , Arpin Dominic , Archambault Denis , Bourgault Steve
Published: May 2020

Life-inspired protein supramolecular assemblies have recently attracted considerable attention for the development of next-generation vaccines to fight against infectious diseases, as well as autoimmune diseases and cancer. Protein self-assembly enables atomic scale precision over the final architecture, with a remarkable diversity of structures and functionalities. Self-assembling protein nanovaccines are associated with numerous advantages, including biocompatibility, stability, molecular specificity and multivalency. Owing to their nanoscale size, proteinaceous nature, symmetrical organization and repetitive antigen display, protein assemblies closely mimic most invading pathogens, serving as danger signals for the immune system. Elucidating how the structural and physicochemical properties of the assemblies modulate the potency and the polarization of the immune responses is critical for bottom-up design of vaccines. In this context, this review briefly covers the fundamentals of supramolecular interactions involved in protein self-assembly and presents the strategies to design and functionalize these assemblies. Examples of advanced nanovaccines are presented, and properties of protein supramolecular structures enabling modulation of the immune responses are discussed. Combining the understanding of the self-assembly process at the molecular level with knowledge regarding the activation of the innate and adaptive immune responses will support the design of safe and effective nanovaccines.
Dietary Habits in Children with Respiratory Allergies: A Single-Center Polish Pilot Study
Authors: Wasilewska Eliza , Małgorzewicz Sylwia , Gruchała-Niedoszytko Marta , Skotnicka Magdalena , Jassem Ewa
Published: May 2020

Background: The rising trend in allergic diseases has developed in parallel with the increasing prevalence of obesity, suggesting a possible association. The links between eating habits and allergies have not been sufficiently clarified. Aim: To evaluate the nutritional status, eating habits, and risk factors of obesity and pulmonary function in children with allergic rhinitis. Materials and methods: We evaluated 106 children with allergic rhinitis (mean age 12.1 ± 3.4 years; M/F 60/46) from the Department of Allergology. Clinical data were collected regarding allergies, physical activity, nutritional status (Bodystat), dietary habits (Food Frequency Questionnaire validated for the Polish population), skin prick test with aeroallergens (Allergopharma), and spirometry (Jaeger). Results: All children suffered from allergic rhinitis; among them, 43 (40.6%) presented symptoms of asthma. There were differences between children with only allergic rhinitis (AR group) and children with both rhinitis and asthma (AA group) in pulmonary function (forced expiratory volume in one second (FEV1) 100 ± 11 vs. 92.1 ± 15.0; p < 0.05). A total of 84 children (79%) presented a normal body mass index (BMI) (10–97 percentile), 8 (7.5%) were underweight, and 14 (13.5%) were overweight or obese. There were no differences in body composition between the AR and AA groups. Incorrect eating habits were demonstrated by most of the children, e.g., consumption of three or fewer meals in a day (38%), sweets every day (44%), snacking between meals every day (80%), and eating meals less than 1 h before bedtime (47%). Compared to the AR group, the AA group was more likely to eat more meals a day (p = 0.04), snack more often (p = 0.04), and eat before sleeping (p = 0.005). Multiple regression analysis showed a significant association between high BMI and snacking between meals and low physical activity (adjusted R2 = 0.97; p < 0.05). Conclusions: The risk factors for obesity in children with allergies include snacking and low physical activity. Most children with respiratory allergies, especially those with asthma, reported incorrect eating habits such as snacking and eating before bedtime. A correlation between pulmonary function and body composition or dietary habits was not found.
Socio-Cultural and Economic Drivers of Plant and Animal Protein Consumption in Malaysia: The SCRiPT Study
Authors: Drewnowski Adam , Mognard Elise , Gupta Shilpi , Ismail Mohd Noor , Karim Norimah A. , Tibère Laurence , Laporte Cyrille , Alem Yasmine , Khusun Helda , Februhartanty Judhiastuty , Anggraini Roselynne , Poulain Jean-Pierre
Published: May 2020

Countries in South East Asia are undergoing a nutrition transition, which typically involves a dietary shift from plant to animal proteins. To explore the main drivers of protein consumption, the SCRiPT (Socio Cultural Research in Protein Transition) study recruited a population sample in Malaysia (N = 1604). Participants completed in-person 24 h dietary recalls and socio-demographic surveys. Energy and nutrient intakes were estimated using Nutritionist Pro. A novel recipe-based frequency count coded protein sources as meat (chicken, beef, pork, and mutton), fish, eggs, dairy, and plants (cereals, pulses, tubers). Dietary intakes and frequencies were examined by gender, age, income, education, ethnicity, religion, and family status, using ANOVAs and general linear models. Energy intakes were 1869 kcal/d for men and 1699 kcal/d for women. Protein intakes were 78.5 g/d for men and 72.5 g/d for women. Higher energy and protein intakes were associated with Chinese ethnicity, higher education and incomes. Frequency counts identified plant proteins in 50% of foods, followed by meat (19%), fish (12%), eggs (12%), and dairy (7%). Most frequent source of meat was chicken (16%) rather than pork or beef (1.5% each). In bivariate analyses, animal protein counts were associated with younger age, higher education and incomes. In mutually adjusted multivariate regression models, animal proteins were associated with education and ethnicity; plant proteins were associated with ethnicity and religion. Protein choices in Malaysia involve socio-cultural as well as economic variables.
Nutritional Strategies to Offset Disuse-Induced Skeletal Muscle Atrophy and Anabolic Resistance in Older Adults: From Whole-Foods to Isolated Ingredients
Authors: Marshall Ryan N. , Smeuninx Benoit , Morgan Paul T. , Breen Leigh
Published: May 2020

Preserving skeletal muscle mass and functional capacity is essential for healthy ageing. Transient periods of disuse and/or inactivity in combination with sub-optimal dietary intake have been shown to accelerate the age-related loss of muscle mass and strength, predisposing to disability and metabolic disease. Mechanisms underlying disuse and/or inactivity-related muscle deterioration in the older adults, whilst multifaceted, ultimately manifest in an imbalance between rates of muscle protein synthesis and breakdown, resulting in net muscle loss. To date, the most potent intervention to mitigate disuse-induced muscle deterioration is mechanical loading in the form of resistance exercise. However, the feasibility of older individuals performing resistance exercise during disuse and inactivity has been questioned, particularly as illness and injury may affect adherence and safety, as well as accessibility to appropriate equipment and physical therapists. Therefore, optimising nutritional intake during disuse events, through the introduction of protein-rich whole-foods, isolated proteins and nutrient compounds with purported pro-anabolic and anti-catabolic properties could offset impairments in muscle protein turnover and, ultimately, the degree of muscle atrophy and recovery upon re-ambulation. The current review therefore aims to provide an overview of nutritional countermeasures to disuse atrophy and anabolic resistance in older individuals.
Nutraceutical Effects of Lycopene in Experimental Varicocele: An “In Vivo” Model to Study Male Infertility
Authors: Antonuccio Pietro , Micali Antonio , Puzzolo Domenico , Romeo Carmelo , Vermiglio Giovanna , Squadrito Violetta , Freni Jose , Pallio Giovanni , Trichilo Vincenzo , Righi Maria , Irrera Natasha , Altavilla Domenica , Squadrito Francesco , Marini Herbert R. , Minutoli Letteria
Published: May 2020

Varicocele is one of the main causes of infertility in men. Oxidative stress and consequently apoptosis activation contribute to varicocele pathogenesis, worsening its prognosis. Natural products, such as lycopene, showed antioxidant and anti-inflammatory effects in several experimental models, also in testes. In this study we investigated lycopene effects in an experimental model of varicocele. Male rats (n = 14) underwent sham operations and were administered with vehicle (n = 7) or with lycopene (n = 7; 1 mg/kg i.p., daily). Another group of animals (n = 14) underwent surgical varicocele. After 28 days, the sham and 7 varicocele animals were euthanized, and both operated and contralateral testes were weighted and processed. The remaining rats were treated with lycopene (1 mg/kg i.p., daily) for 30 days. Varicocele rats showed reduced testosterone levels, testes weight, Bcl-2 mRNA expression, changes in testes structure and increased malondialdehyde levels and BAX gene expression. TUNEL (Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling) assay showed an increased number of apoptotic cells. Treatment with lycopene significantly increased testosterone levels, testes weight, and Bcl-2 mRNA expression, improved tubular structure and decreased malondialdehyde levels, BAX mRNA expression and TUNEL-positive cells. The present results show that lycopene exerts beneficial effects in testes, and suggest that supplementation with the tomato-derived carotenoid might be considered a novel nutraceutical strategy for the treatment of varicocele and male infertility.
Blackcurrant (Ribes nigrum) Extract Prevents Dyslipidemia and Hepatic Steatosis in Ovariectomized Rats
Authors: Nanashima Naoki , Horie Kayo , Yamanouchi Kanako , Tomisawa Toshiko , Kitajima Maiko , Oey Indrawati , Maeda Hayato
Published: May 2020

Estrogen is involved in lipid metabolism. Menopausal women with low estrogen secretion usually gain weight and develop steatosis associated with abnormal lipid metabolism. A previous study showed that blackcurrant (Ribes nigrum L.) extract (BCE) had phytoestrogen activity. In this study, we examined whether BCE improved lipid metabolism abnormalities and reduced liver steatosis in ovariectomized rats, as a menopausal animal model. Twelve-week-old ovariectomized (OVX) rats were fed a regular diet (Ctrl) or a 3% BCE supplemented diet while sham rats were fed a regular diet for three months. Body weight, visceral fat weight, levels of serum triglycerides, total cholesterol, and LDL cholesterol decreased in the BCE-treated OVX and sham rats, but not in OVX Ctrl rats. The results of hematoxylin and eosin staining revealed that BCE decreased the diameters of adipocytes and the nonalcoholic fatty liver disease activity score. Furthermore, quantitative RT-PCR indicated a decreased expression of hepatitis-related genes, such as tumor necrosis factor-α, IL-6, and IL-1β in OVX rats after BCE treatment. This is the first study that reported improvement of lipid metabolism abnormalities in OVX rats by BCE administration. These results suggest that the intake of BCE alleviated dyslipidemia and prevented nonalcoholic steatohepatitis during menopause in this animal model.
Barcoding of the Genus Culicoides (Diptera: Ceratopogonidae) in Austria—An Update of the Species Inventory Including the First Records of Three Species in Austria
Authors: Zittra Carina , Wöss Günther , Van der Vloet Lara , Bakran-Lebl Karin , Shahi Barogh Bita , Sehnal Peter , Fuehrer Hans-Peter
Published: May 2020

Ceratopogonidae are small nematoceran Diptera with a worldwide distribution, consisting of more than 5400 described species, divided into 125 genera. The genus Culicoides is known to comprise hematophagous vectors of medical and veterinary importance. Diseases transmitted by Culicoides spp. Such as African horse sickness virus, Bluetongue virus, equine encephalitis virus (Reoviridae) and Schmallenberg virus (Bunyaviridae) affect large parts of Europe and are strongly linked to the spread and abundance of its vectors. However, Culicoides surveillance measures are not implemented regularly nor in the whole of Austria. In this study, 142 morphologically identified individuals were chosen for molecular analyses (barcoding) of the mitochondrial cytochrome c oxidase subunit I gene (mt COI). Molecular analyses mostly supported previous morphologic identification. Mismatches between results of molecular and morphologic analysis revealed three new Culicoides species in Austria, Culicoides gornostaevae Mirzaeva, 1984, which is a member of the Obsoletus group, C. griseidorsum Kieffer, 1918 and C. pallidicornis Kieffer, 1919 as well as possible cryptic species. We present here the first Austrian barcodes of the mt COI region of 26 Culicoides species and conclude that barcoding is a reliable tool with which to support morphologic analysis, especially with regard to the difficult to identify females of the medically and economically important genus Culicoides.
Role of Hydrogen Peroxide Vapor (HPV) for the Disinfection of Hospital Surfaces Contaminated by Multiresistant Bacteria
Authors: Totaro Michele , Casini Beatrice , Profeti Sara , Tuvo Benedetta , Privitera Gaetano , Baggiani Angelo
Published: May 2020

The emergence of multiresistant bacterial strains as agents of healthcare-related infection in hospitals has prompted a review of the control techniques, with an added emphasis on preventive measures, namely good clinical practices, antimicrobial stewardship, and appropriate environmental cleaning. The latter item is about the choice of an appropriate disinfectant as a critical role due to the difficulties often encountered in obtaining a complete eradication of environmental contaminations and reservoirs of pathogens. The present review is focused on the effectiveness of hydrogen peroxide vapor, among the new environmental disinfectants that have been adopted. The method is based on a critical review of the available literature on this topic
Wide Range of the Prevalence and Viral Loads of Porcine Circovirus Type 3 (PCV3) in Different Clinical Materials from 21 Polish Pig Farms
Authors: Woźniak Aleksandra , Miłek Dagmara , Stadejek Tomasz
Published: May 2020

Porcine circovirus type 3 (PCV3) was described in different clinical cases and healthy pigs. However, little is known about its circulation in pig farms. In order to assess PCV3 prevalence in 21 Polish farms, serum, feces, and oral fluid samples were examined by quantitative real-time PCR. In total, 1451 pairs of serum and feces from the same animals, as well as 327 samples of oral fluids were analyzed. The results showed that PCV3 is more commonly detected in oral fluids (37.3% positives) than in serum (9.7% positives) or feces (15.0% positives) samples. The viral loads detected in these materials ranged from 102.5–107.2 genome equivalent copies/mL. Although in most farms PCV3 was detected post weaning, in nine farms, the virus was also found in groups of suckling piglets, and in six of them viremia was detected. In four farms with reproductive failure, fetal materials were also obtained. PCV3 was detected in 36.0% of fetuses or stillborn piglets (9/25) with viral loads of 103.1–1010.4 genome equivalent copies/mL. In summary, the virus circulation may show different patterns, and congenital or early infection is not uncommon. Precise quantification of PCV3 loads in clinical materials seems to be necessary for the study and diagnosis of the infection.
Constructing Auxin-Inducible Degron Mutants Using an All-in-One Vector
Authors: Yesbolatova Aisha , Saito Yuichiro , Kanemaki Masato T.
Published: May 2020

Conditional degron-based methods are powerful for studying protein function because a degron-fused protein can be rapidly and efficiently depleted by adding a defined ligand. Auxin-inducible degron (AID) is a popular technology by which a degron-fused protein can be degraded by adding an auxin. However, compared with other technologies such as dTAG and HaloPROTAC, AID is complicated because of its two protein components: OsTIR1 and mAID (degron). To simplify the use of AID in mammalian cells, we constructed bicistronic all-in-one plasmids that express OsTIR1 and a mAID-fused protein using a P2A self-cleavage sequence. To generate a HeLa mutant line for the essential replication factor MCM10, we transfected a CRISPR-knockout plasmid together with a bicistronic plasmid containing mAID-fused MCM10 cDNA. After drug selection and colony isolation, we successfully isolated HeLa mutant lines, in which mAID–MCM10 was depleted by the addition of indole-3-acetic acid, a natural auxin. The bicistronic all-in-one plasmids described in this report are useful for controlling degradation of a transgene-derived protein fused with mAID. These plasmids can be used for the construction of conditional mutants by combining them with a CRISPR-based gene knockout.
Optimization of Curcumin Nanocrystals as Promising Strategy for Nose-to-Brain Delivery Application
Authors: Bonaccorso Angela , Gigliobianco Maria Rosa , Pellitteri Rosalia , Santonocito Debora , Carbone Claudia , Di Martino Piera , Puglisi Giovanni , Musumeci Teresa
Published: May 2020

Intranasal (IN) drug delivery is recognized to be an innovative strategy to deliver drugs to the Central Nervous System. One of the main limitations of IN dosing is the low volume of drug that can be administered. Accordingly, two requirements are necessary: the drug should be active at a low dosage, and the drug solubility in water must be high enough to accommodate the required dose. Drug nanocrystals may overcome these limitations; thus, curcumin was selected as a model drug to prepare nanocrystals for potential IN administration. With this aim, we designed curcumin nanocrystals (NCs) by using Box Behnken design. A total of 51 formulations were prepared by the sonoprecipitation method. Once we assessed the influence of the independent variables on nanocrystals’ mean diameter, the formulation was optimized based on the desirability function. The optimized formulation was characterized from a physico-chemical point of view to evaluate the mean size, zeta potential, polidispersity index, pH, osmolarity, morphology, thermotropic behavior and the degree of crystallinity. Finally, the cellular uptake of curcumin and curcumin NCs was evaluated on Olfactory Ensheathing Cells (OECs). Our results showed that the OECs efficiently took up the NCs compared to the free curcumin, showing that NCs can ameliorate drug permeability.
The Role of Community Pharmacists in the Detection of Clinically Relevant Drug-Related Problems in Chronic Kidney Disease Patients
Authors: Mongaret Céline , Aubert Léa , Lestrille Amélie , Albaut Victorine , Kreit Pierre , Herlem Emmanuelle , Noel Natacha , Touré Fatouma , Lallier François , Slimano Florian , MIRPhO Investigators Study the
Published: May 2020

Community pharmacists (CPs) have traditionally had limited access to patients’ estimated glomerular filtration rate (eGFR) during the medication-dispensing process. The increasing access to shared electronic health records is making eGFR available, but the skills needed to detect and manage clinically relevant drug-related problems (DRPs) are poorly documented. The primary objective of this study was to investigate the role of CPs in the medication-dispensation process for elderly patients with renal impairment. A total of 70 CPs participated in this 6 month study. Community pharmacists asked all patients ≥65 years to bring their laboratory test values for the next medication-dispensing process. Drug-related problem detection rates were compared between CPs (prospective period) and expert pharmacists (retrospectively). The clinical relevance of each DRP was assessed by nephrologists and general practitioners using an appropriate tool. Community pharmacists recruited n = 442 patients with eGFR < 60 mL/min/1.73 m2 and detected n = 99 DRPs, whereas expert pharmacists detected n = 184 DRPs. The most frequently detected DRPs were dosage problems and contraindications. According to assessment by clinicians, CPs and expert pharmacists identified 54.0% and 84.7% of clinically relevant DRPs, respectively. This study suggests a positive impact of the systematic availability of eGFR to CPs on the detection of several DRPs with clinical relevance.
Investigation of Indigenous Arbuscular Mycorrhizal Performance Using a Lotus japonicus Mycorrhizal Mutant
Authors: Teranishi Taisuke , Kobae Yoshihro
Published: May 2020

Most plants are usually colonized with arbuscular mycorrhiza fungi (AMF) in the fields. AMF absorb mineral nutrients, especially phosphate, from the soil and transfer them to the host plants. Inoculation with exotic AMF is thought to be effective when indigenous AMF performance is low; however, there is no method for evaluating the performance of indigenous AMF. In this study, we developed a method to investigate the performance of indigenous AMF in promoting plant growth. As Lotus japonicus mutant (str) that are unable to form functional mycorrhizal roots were considered to be symbiosis negative for indigenous mycorrhizal performance, we examined the growth ratios of wild-type and str mycorrhizal mutant using 24 soils. Each soil had its own unique indigenous mycorrhizal performance, which was not directly related to the colonization level of indigenous AMF or soil phosphate level. The low indigenous mycorrhizal performance could not be compensated by the inoculation of exotic AMF. Importantly, indigenous mycorrhizal performance was never negative; however, the inoculation of exotic AMF into the same soil led to both positive and negative performances. These results suggest that indigenous mycorrhizal performance is affected by soil management history and is basically harmless to the plant.
Designing a Clean Label Fish Patty with Olive, Citric, Pomegranate, or Rosemary Extracts
Authors: Martínez-Zamora Lorena , Ros Gaspar , Nieto Gema
Published: May 2020

The natural functional ingredients derived from pomegranate (41.4% punicalagin), rosemary (5.8% carnosic acid and carnosol), hydroxytyrosol (7.3%), and citrus (55% hesperidin) fruits were combined separately with acerola (17% vitamin C) and essential oils rich in fatty acids (45% α-linolenic (ALA) and 40% docosahexaenoic (DHA)) provide a natural substitute of synthetic preservatives for fish patties, avoiding E-numbers on labels. Microbiological and physicochemical properties of the formulations were examined, sensory analysis was conducted, and changes in their shelf life due to storage for 14 days under chilled storage, adding these ingredients, were determined. The results obtained showed that the fish patties reported a high level of protein (14%), low fat (<2%), with a high contribution of phosphorus and selenium minerals, and higher levels of ALA up to 40% (in the case of rosemary extract (R)) and DHA by 30% (Ct), compared to the Control sample. The fish patties suffered microbiological, flavor, and odor spoilage and rapid lipid oxidation associated with rancidity. It can be said that the fish preparations have a duration of less than 7 days (between 4–6 days), except for the preparation with pomegranate extract (P) that has a longer life, from 7 to 11 days. Consequently, replacing synthetic additives by natural extracts offers a new clean label product with potential health benefits that resembles the commercial fish patties.
A Study on the Effect of Macro- and Micro- Nutrients on Nannochloropsis oceanica Growth, Fatty Acid Composition and Magnetic Harvesting Efficiency
Authors: Savvidou Maria G. , Boli Elenitsa , Logothetis Dimitrios , Lymperopoulou Theopisti , Ferraro Angelo , Louli Vasiliki , Mamma Diomi , Kekos Dimitris , Magoulas Kostis , Kolisis Fragiskos N.
Published: May 2020

The effect of iron, manganese, phosphorus and nitrogen on growth and lipid synthesis of the microalgae Nannochloropsis oceanica CCMP1779, as well as their impact on the magnetic harvesting efficiency, are examined under their depriving cell culture conditions. Herein, it is demonstrated that nitrogen and manganese depletion primarily reduced cell growth while phosphorus and iron restriction led to higher dry biomass. Subsequently, the role of those nutrients on fatty acids profile was examined. Phosphorus and nitrogen restriction resulted in lower and higher lipid content, respectively. High amounts of polyunsaturated fatty acids like eicosapentaenoic acid are produced under iron and manganese depletion. Phosphorus deprivation favors monounsaturated fatty acids such as C18:1 and C16:1, while nitrogen restriction favors saturated fatty acid production like C14:0, C16:0 and C18:0. Since the presence/absence of macro- and micro-elements may affect the overall electrostatic charges on the outmost microalgae surface, it was also analyzed how these elements affect the magnetic harvesting efficiency. Results showed that phosphorus deprivation led to the best magnetic harvesting efficiency of N. oceanica cells (93%) as compared to other nutrient starvation as well as standard medium.
Plant Viruses Infecting Solanaceae Family Members in the Cultivated and Wild Environments: A Review
Authors: Hančinský Richard , Mihálik Daniel , Mrkvová Michaela , Candresse Thierry , Glasa Miroslav
Published: May 2020

Plant viruses infecting crop species are causing long-lasting economic losses and are endangering food security worldwide. Ongoing events, such as climate change, changes in agricultural practices, globalization of markets or changes in plant virus vector populations, are affecting plant virus life cycles. Because farmer’s fields are part of the larger environment, the role of wild plant species in plant virus life cycles can provide information about underlying processes during virus transmission and spread. This review focuses on the Solanaceae family, which contains thousands of species growing all around the world, including crop species, wild flora and model plants for genetic research. In a first part, we analyze various viruses infecting Solanaceae plants across the agro-ecological interface, emphasizing the important role of virus interactions between the cultivated and wild zones as global changes affect these environments on both local and global scales. To cope with these changes, it is necessary to adjust prophylactic protection measures and diagnostic methods. As illustrated in the second part, a complex virus research at the landscape level is necessary to obtain relevant data, which could be overwhelming. Based on evidence from previous studies we conclude that Solanaceae plant communities can be targeted to address complete life cycles of viruses with different life strategies within the agro-ecological interface. Data obtained from such research could then be used to improve plant protection methods by taking into consideration environmental factors that are impacting the life cycles of plant viruses.
Improvement of the Mechanical Properties of Thermosetting-Binding-System-Based Composites by Means of Kneading Procedure Modification and Composite Formulation
Authors: Šahinović Adis , Mušič Branka
Published: May 2020

By understanding the effects of the physical properties of individual input materials (e.g., binding system) on the physical and thermal properties of a composite material, the latter can be engineered in advance according to the desired properties and application. Often, a need to replace a specific component in a composite material arises, due to various reasons such as high raw material prices, product price reduction, environmental issues, improvement of properties, and others. In this study, we focused on the substitution of a phenolic novolac resin binding system and the reduction of compounding process temperature in combination with material throughput and screw speed variation of a phenolic-novolac-resin-based composite material, manufactured by kneading process using a co-kneader single screw extruder. Modifications were carried out in the interest of reducing production process cost and positive environmental effect due to reduction of energy consumption in the compounding process. We achieved great success in improvement of mechanical properties with all four substituted phenolic molding compounds (PMCs), while the decrease in thermal stability was the lowest for PMCs prepared at higher screw speeds and material throughput. The results indicated that higher screw speeds produce the best combination of mechanical and thermal properties of PMCs.
Experimental and Statistical Validation of Data on Mesh-Coupled Annular Distributor Design for Swirling Fluidized Beds
Authors: Shukrullah Shazia , Naz Muhammad Yasin , Ghaffar Abdul , Khan Yasin , Al-Arainy Abdulrehman Ali , Meer Rashed
Published: May 2020

In this study, velocimetry and statistical analyses were conducted on a swirling fluidized bed. A bed of spherical particles (4 mm) was fluidized by using an annular distributor covered with mesh. The angles of rectangular blades in the distributor were set at 30°, 45°, 60°, 75° and 90°, and the cell size of the mesh cover was 2.5 × 2.5 mm2. The weight was varied from 500 to 1250 g and the effect of each variable on bed velocity response was quantified through velocimetry and statistical analysis. The statistical analysis was conducted using NCSS statistical software. The blade angle, bed weight and superficial velocity for 4 mm particles were statistically optimized at 750 g, 58.26° and 1.45 m/s, respectively. On the experimental side, these parameters have been optimized at 750 g, 60° and 1.41 m/s, respectively. A small difference of 1.74° was noticed in experimental and statistical predictions for the blade angle. The bed weights and superficial velocities were found to be same in both cases. The confidence interval (95%) for bed velocity was proposed in the range of 0.513 to 0.519 m/s. The experimentally optimized bed velocity remained within the proposed range. The well-agreeing results indicate good practical value of distributor design and high precision of the experimental measurements.
Fluid-Structure Interaction Analysis of Subject-Specific Mitral Valve Regurgitation Treatment with an Intra-Valvular Spacer
Authors: Toma Milan , Einstein Daniel , Bloodworth Charles , Kohli Keshav , Cochran Richard , Kunzelman Karyn , Yoganathan Ajit
Published: May 2020

Mitral regurgitation imposes a significant symptomatic burden on patients who are not candidates for conventional surgery. For these patients, transcatheter repair and replacement devices are emerging as alternative options. One such device is an intravalvular balloon or spacer that is inserted between the mitral valve leaflets to fill the gap that gives rise to mitral regurgitation. In this study, we apply a large-deformation fluid-structure interaction analysis to a fully 3D subject-specific mitral valve model to assess the efficacy of the intra-valvular spacer for reducing mitral regurgitation severity. The model includes a topologically 3D subvalvular apparatus with unprecedented detail. Results show that device fixation and anchoring at the location of the subject’s regurgitant orifice is imperative for optimal reduction of mitral regurgitation.
Correlations between Complexity and Entanglement in a One-Dimensional XY Model
Authors: Apel V. M. , Mundarain Douglas , Pennini Flavia , Plastino Angelo
Published: May 2020

Many people believe that the study of complex quantum systems may be simplified by first analyzing the static and dynamic entanglement present in those systems [Phys. Rev. A 66 (2002) 032110]. In this paper, we attempt to complement such notion by adding an order–disorder quantifier called statistical complexity and studying how it is correlated with the degree of entanglement as measured by the concurrence quantifier. We perform such an analysis with reference to a representative system chosen from condensed matter theory, the so-called X Y model. Some interesting insight is obtained as the concurrence and the complexity become correlated in an unexpected fashion.
Surgical Treatment of Cavernous Sinus Cavernomas: Evidence from Vietnam
Authors: Nguyen Duc-Anh , Nguyen Hao The , Duong Thang Van , Pham Binh Hoa , Vo Hoang-Long
Published: May 2020

Cavernous sinus cavernomas, a rare vascular malformation, represents 3% of all benign cavernous sinus tumors. Both clinical and radiological signs are important for differentiating this condition from other cavernous sinus diseases. The best treatment is radical removal tumor surgery; however, due to the tumor being located in the cavernous sinus, there are many difficulties in the surgery. We report a case of a 35-year-old female who only presented sporadical headache. After serial magnetic resonance imaging acquisitions, a tumor measuring 30 mm in the left cavernous sinus and heterogenous enhencement was observed. Then, the patient underwent an operation with an extradural basal temporal approach. Postoperatively, the tumor was safely gross total removed. The patient developed left oculomotor nerve palsy but fully recovered after 3 months of acupunture treatment, and developed persistent left maxillofacial paresthesia. The surgical treatment for cavernous sinus cavernomas may be considered a best choice regarding safety and efficiency.
Land Use Simulation of Guangzhou Based on Nighttime Light Data and Planning Policies
Authors: Lao Jieying , Wang Cheng , Wang Jinliang , Pan Feifei , Xi Xiaohuan , Liang Lei
Published: May 2020

With the implementation processes of strategies such as Guangdong-Hong Kong-Macau Greater Bay Area’s coordinated development and “Belt and Road Initiative” initiative, the planning policies had produced a significant influence on land use distributions in Guangzhou. In this paper, we employ nighttime light (NTL) information as a proxy indicator of gross domestic product(GDP), and a future land use simulation model (FLUS) to simulate the land use patterns in Guangzhou from 2015 to 2018 and 2018 to 2035 by incorporating planning policies. The results show that: (1) the accuracy of simulation result from 2015 to 2018 based on National Polar-orbiting Partnership, Visible Infrared Imaging Radiometer Suite (NPP/VIIRS) is higher than that based on GDP; (2) by incorporating planning policies into the model can better identify the potential spatial distribution of urban land and make the simulated results more consistent with the actual urban land development trajectory. This study demonstrates that NTL is a suitable and feasible proxy indicator of GDP for the land use simulations, providing a scientific basis for the development of urban planning and construction policy.
Automatic Processing of Aerial LiDAR Data to Detect Vegetation Continuity in the Surroundings of Roads
Authors: Novo Ana , Fariñas-Álvarez Noelia , Martínez-Sánchez Joaquin , González-Jorge Higinio , Lorenzo Henrique
Published: May 2020

The optimization of forest management in the surroundings of roads is a necessary task in term of wildfire prevention and the mitigation of their effects. One of the reasons why a forest fire spreads is the presence of contiguous flammable material, both horizontally and vertically and, thus, vegetation management becomes essential in preventive actions. This work presents a methodology to detect the continuity of vegetation based on aerial Light Detection and Ranging (LiDAR) point clouds, in combination with point cloud processing techniques. Horizontal continuity is determined by calculating Cover Canopy Fraction (CCF). The results obtained show 50% of shrubs presence and 33% of trees presence in the selected case of study, with an error of 5.71%. Regarding vertical continuity, a forest structure composed of a single stratum represents 81% of the zone. In addition, the vegetation located in areas around the roads were mapped, taking into consideration the distances established in the applicable law. Analyses show that risky areas range from a total of 0.12 ha in a 2 m buffer and 0.48 ha in a 10 m buffer, representing a 2.4% and 9.5% of the total study area, respectively.
Performance Evaluation of the Multiple Quantile Regression Model for Estimating Spatial Soil Moisture after Filtering Soil Moisture Outliers
Authors: Jung Chunggil , Lee Yonggwan , Lee Jiwan , Kim Seongjoon
Published: May 2020

The spatial distribution of soil moisture (SM) was estimated by a multiple quantile regression (MQR) model with Terra Moderate Resolution Imaging Spectroradiometer (MODIS) and filtered SM data from 2013 to 2015 in South Korea. For input data, observed precipitation and SM data were collected from the Korea Meteorological Administration and various institutions monitoring SM. To improve the work of a previous study, prior to the estimation of SM, outlier detection using the isolation forest (IF) algorithm was applied to the observed SM data. The original observed SM data resulted in IF_SM data following outlier detection. This study obtained an average data removal rate of 20.1% at 58 stations. For various reasons, such as instrumentation, environment, and random errors, the original observed SM data contained approximately 20% uncertain data. After outlier detection, this study performed a regression analysis by estimating land surface temperature quantiles. The soil characteristics were considered through reclassification into four soil types (clay, loam, silt, and sand), and the five-day antecedent precipitation was considered in order to estimate the regression coefficient of the MQR model. For all soil types, the coefficient of determination (R2) and root mean square error (RMSE) values ranged from 0.25 to 0.77 and 1.86% to 12.21%, respectively. The MQR results showed a much better performance than that of the multiple linear regression (MLR) results, which yielded R2 and RMSE values of 0.20 to 0.66 and 1.08% to 7.23%, respectively. As a further illustration of improvement, the box plots of the MQR SM were closer to those of the observed SM than those of the MLR SM. This result indicates that the cumulative distribution functions (CDF) of MQR SM matched the CDF of the observed SM. Thus, the MQR algorithm with outlier detection can overcome the limitations of the MLR algorithm by reducing both the bias and variance.
Feasibility Study Using UAV Aerial Photogrammetry for a Boundary Verification Survey of a Digitalized Cadastral Area in an Urban City of Taiwan
Authors: Chio Shih-Hong , Chiang Cheng-Chu
Published: May 2020

In conducting land boundary verification surveys in digitalized cadastral areas in Taiwan, possible parcel points must be surveyed. These points are employed in the overlap analysis and map registration of possible parcel points and digitalized cadastral maps to identify the coordinates of parcel points. Based on the computed horizontal distance and angle between control points and parcel points, parcels are staked out using ground surveys. Most studies survey possible parcel points using ground surveys with, for example, total stations. Compared with ground surveys, UAV (Unmanned Aerial Vehicle) aerial photogrammetry can provide more possible parcel points. Thus, an overlap analysis of digitalized cadastral maps, combined with the collection of possible parcel points, will be more comprehensive. In this study, a high-quality-medium format camera, with a 55 mm focal length, was carried on a rotary UAV to take images, with a 3 cm ground sampling distance (GSD), flying 300 m above the ground. The images were taken with an 80% end-lap and side-lap to increase the visibility of the terrain details for stereo-mapping. According to the test conducted in this study, UAV aerial photogrammetry can accurately provide supplementary control points and assist in the boundary verification of digitalized cadastral areas in Taiwan.
Coastal Wetland Mapping Using Ensemble Learning Algorithms: A Comparative Study of Bagging, Boosting and Stacking Techniques
Authors: Wen Li , Hughes Michael
Published: May 2020

Coastal wetlands are a critical component of the coastal landscape that are increasingly threatened by sea level rise and other human disturbance. Periodically mapping wetland distribution is crucial to coastal ecosystem management. Ensemble algorithms (EL), such as random forest (RF) and gradient boosting machine (GBM) algorithms, are now commonly applied in the field of remote sensing. However, the performance and potential of other EL methods, such as extreme gradient boosting (XGBoost) and bagged trees, are rarely compared and tested for coastal wetland mapping. In this study, we applied the three most widely used EL techniques (i.e., bagging, boosting and stacking) to map wetland distribution in a highly modified coastal catchment, the Manning River Estuary, Australia. Our results demonstrated the advantages of using ensemble classifiers to accurately map wetland types in a coastal landscape. Enhanced bagging decision trees, i.e., classifiers with additional methods to increasing ensemble diversity such as RF and weighted subspace random forest, had comparably high predictive power. For the stacking method evaluated in this study, our results are inconclusive, and further comprehensive quantitative study is encouraged. Our findings also suggested that the ensemble methods were less effective at discriminating minority classes in comparison with more common classes. Finally, the variable importance results indicated that hydro-geomorphic factors, such as tidal depth and distance to water edge, were among the most influential variables across the top classifiers. However, vegetation indices derived from longer time series of remote sensing data that arrest the full features of land phenology are likely to improve wetland type separation in coastal areas.
Evaluation of Himawari-8/AHI, MERRA-2, and CAMS Aerosol Products over China
Authors: Zhang Taixin , Zang Lin , Mao Feiyue , Wan Youchuan , Zhu Yannian
Published: May 2020

Reliable aerosol optical depth (AOD) data with high spatial and temporal resolutions are needed for research on air pollution in China. AOD products from the Advanced Himawari Imager (AHI) onboard the geostationary Himawari-8 satellite and reanalysis datasets make it possible to capture diurnal variations of aerosol loadings. However, due to the different retrieval methods, their applicability may vary with different space and time. Thus, in this study, taking the measured AOD at the Aerosol Robotic NETwork (AERONET) stations as the gold standard, the performance of the latest AHI hourly AOD product (i.e., L3 AOD) was evaluated and then compared with that of two reanalysis AOD datasets offered by Modern-Era Retrospective Analysis for Research and Applications, Version 2 (MERRA-2) and Copernicus Atmosphere Monitoring Service (CAMS), respectively, covering from July 2015 to December 2017 over China. For all the matchups, AHI AOD shows the highest robustness with a high correlation (R) of 0.82, low root-mean-square error (RMSE) of 0.23, and moderate mean absolute relative error (MARE) of 0.56. Although MERRA-2 and CAMS products both have lower R values (0.74, 0.72) and higher RMSE (0.28, 0.26), the former is slightly better than the latter. Accuracy of AOD products could be mainly affected by the pollution level and less affected by particle size distribution. Comparisons among these AOD products imply that AHI AOD is more reliable in regions with high pollution levels, such as central and eastern China, while in the northern and western part, MERRA-2 AOD seems more satisfying. The performance of all the three AOD products presents a significant diurnal variety, as indicated by the highest accuracy in the morning for AHI and at noon for reanalysis data. Moreover, due to various pollution distribution patterns and meteorological conditions, there are distinct seasonal characteristics in the performance of AOD products for different regions.