bluuebunny/crossref_metadata_2025 huggingface dataset:
Universal Access to Xpert MTB/RIF Testing for Diagnosis of Tuberculosis in Uzbekistan: How Well Are We Doing?
Authors: Turaev Laziz , Kumar Ajay , Nabirova Dilyara , Alaverdyan Sevak , Parpieva Nargiza , Abdusamatova Barno
Published: March 2021

As per national guidelines in Uzbekistan, all presumptive tuberculosis patients should be tested using the Xpert MTB/RIF assay for diagnosing tuberculosis. There is no published evidence how well this is being implemented. In this paper, we report on the Xpert coverage among presumptive tuberculosis patients in 2018 and 2019, factors associated with non-testing and delays involved. Analysis of national aggregate data indicated that Xpert testing increased from 24% in 2018 to 46% in 2019, with variation among the regions: 21% in Tashkent region to 100% in Karakalpakstan. In a cohort (January–March 2019) constituted of 40 randomly selected health facilities in Tashkent city and Bukhara region, there were 1940 patients of whom 832 (43%, 95% confidence interval (CI): 41–45%) were not Xpert-tested. Non-testing was significantly higher in Bukhara region (73%) compared to Tashkent city (28%). In multivariable analysis, patient’s age, distance between primary health centre (PHC) and Xpert laboratory, diagnostic capacity and site of PHC were associated with non-testing. The median (interquartile range) duration from date of initial visit to PHC to receiving results was 1 (1–2) day in Tashkent city compared to 3 (1–6) days in Bukhara region (p-value < 0.001). While there is commendable progress, universal access to Xpert testing is not a reality yet.
Ocular Involvement of SARS-CoV-2 in a Polish Cohort of COVID-19-Positive Patients
Authors: Dolar-Szczasny Joanna , Toro Mario D. , Dworzańska Anna , Wójtowicz Tomasz , Korona-Glowniak Izabela , Sawicki Rafał , Boguszewska Anastazja , Polz-Dacewicz Małgorzata , Tomasiewicz Krzysztof , Załuska Wojciech , Rejdak Robert , Bagnoli Paola , Rusciano Dario
Published: March 2021

The coronavirus SARS-CoV-2 responsible for the current human COVID-19 pandemic has shown tropism toward different organs with variable efficiency, eyes included. The purpose of this study has been to investigate the presence of detectable SARS-CoV-2 infection in ocular swabs in patients affected by COVID-19. A consecutive series of 74 COVID-19-positive patients (age 21–89) were enrolled at two Polish COVID-19 hospitals for 4 months and were characterized by PCR for the presence of the SARS-CoV-2 genetic material in nasopharyngeal (NP) and ocular swabs, while their respiratory and ocular symptoms were noted. Almost 50% of them presented with severe/critical respiratory involvement, and some degree of eye disease. No tight correlation was observed between the presence of ocular and respiratory symptoms. Three male patients presenting with severe/critical lung disease tested positive in ocular swab, however with mild/moderate ocular symptoms. In conclusion, our study lends further support to the view that overt ocular infection by the SARS-CoV-2 virus is not such a frequent occurrence.
Exploring the Emerging Domain of Research on Video Game Live Streaming in Web of Science: State of the Art, Changes and Trends
Authors: Cabeza-Ramírez Luis Javier , Fuentes-García Fernando J. , Muñoz-Fernandez Guzmán A.
Published: March 2021

In recent years, interest in video game live streaming services has increased as a new communication instrument, social network, source of leisure, and entertainment platform for millions of users. The rise in this type of service has been accompanied by an increase in research on these platforms. As an emerging domain of research focused on this novel phenomenon takes shape, it is necessary to delve into its nature and antecedents. The main objective of this research is to provide a comprehensive reference that allows future analyses to be addressed with greater rigor and theoretical depth. In this work, we developed a meta-review of the literature supported by a bibliometric performance and network analysis (BPNA). We used the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) protocol to obtain a representative sample of 111 published documents since 2012 and indexed in the Web of Science. Additionally, we exposed the main research topics developed to date, which allowed us to detect future research challenges and trends. The findings revealed four specializations or subdomains: studies focused on the transmitter or streamer; the receiver or the audience; the channel or platform; and the transmission process. These four specializations add to the accumulated knowledge through the development of six core themes that emerge: motivations, behaviors, monetization of activities, quality of experience, use of social networks and media, and gender issues.
Courage, Career Adaptability, and Readiness as Resources to Improve Well-Being during the University-to-Work Transition in Italy
Authors: Magnano Paola , Lodi Ernesto , Zammitti Andrea , Patrizi Patrizia
Published: March 2021

College students approaching a university degree can experience a critical period in their career development path that could affect their well-being. The main aim of this study was to examine the role of courage, career adaptability, and professional readiness as protective factors toward life satisfaction and flourishing during the university-to-work transition. These psychosocial resources could be useful to cope with the recent transformations of the labor market. The study involved 352 Italian university students (M = 100; F = 252), aged from 21 to 29 years (M = 23.57; SD = 2.37), attending the last year of their degree course. The results of the mediation analysis showed that courage plays a mediating role between career transition readiness and career adaptability, on one hand, with well-being indicators as outcomes. The results are discussed, providing some suggestions on practical implications for career interventions to support college students during the university-to-work transition.
Computational Geometry-Based Surface Reconstruction for Volume Estimation: A Case Study on Magnitude-Frequency Relations for a LiDAR-Derived Rockfall Inventory
Authors: DiFrancesco Paul-Mark , Bonneau David A. , Hutchinson D. Jean
Published: March 2021

Key to the quantification of rockfall hazard is an understanding of its magnitude-frequency behaviour. Remote sensing has allowed for the accurate observation of rockfall activity, with methods being developed for digitally assembling the monitored occurrences into a rockfall database. A prevalent challenge is the quantification of rockfall volume, whilst fully considering the 3D information stored in each of the extracted rockfall point clouds. Surface reconstruction is utilized to construct a 3D digital surface representation, allowing for an estimation of the volume of space that a point cloud occupies. Given various point cloud imperfections, it is difficult for methods to generate digital surface representations of rockfall with detailed geometry and correct topology. In this study, we tested four different computational geometry-based surface reconstruction methods on a database comprised of 3668 rockfalls. The database was derived from a 5-year LiDAR monitoring campaign of an active rock slope in interior British Columbia, Canada. Each method resulted in a different magnitude-frequency distribution of rockfall. The implications of 3D volume estimation were demonstrated utilizing surface mesh visualization, cumulative magnitude-frequency plots, power-law fitting, and projected annual frequencies of rockfall occurrence. The 3D volume estimation methods caused a notable shift in the magnitude-frequency relations, while the power-law scaling parameters remained relatively similar. We determined that the optimal 3D volume calculation approach is a hybrid methodology comprised of the Power Crust reconstruction and the Alpha Solid reconstruction. The Alpha Solid approach is to be used on small-scale point clouds, characterized with high curvatures relative to their sampling density, which challenge the Power Crust sampling assumptions.
Effectiveness of Memorizing an Animated Route—Comparing Satellite and Road Map Differences in the Eye-Tracking Study
Authors: Cybulski Paweł
Published: March 2021

There is no consensus on the importance of satellite images in the process of memorizing a route from a map image, especially if the route is displayed on the Internet using dynamic (animated) cartographic visualization. In modern dynamic maps built with JavaScript APIs, background layers can be easily altered by map users. The animation attracts people’s attention better than static images, but it causes some perceptual problems. This study examined the influence of the number of turns on the effectiveness (correctness) and efficiency of memorizing the animated route on different cartographic backgrounds. The routes of three difficulty levels, based on satellite and road background, were compared. The results show that the satellite background was not a significant factor influencing the efficiency and effectiveness of route memorizing. Recordings of the eye movement confirmed this. The study reveals that there were intergroup differences in participants’ visual behavior. Participants who described their spatial abilities as “very good” performed better (in terms of effectiveness and efficiency) in route memorizing tasks. For future research, there is a need to study route variability and its impact on participants’ performance. Moreover, future studies should involve differences in route visualization (e.g., without and with ephemeral or permanent trail).
Temporal and Spatial Variation of Anthropogenic Heat in the Central Urban Area: A Case Study of Guangzhou, China
Authors: Peng Ting , Sun Caige , Feng Shanshan , Zhang Yongdong , Fan Fenglei
Published: March 2021

The urban heat island effect caused by the rapid increase in urban anthropogenic heat has gradually become an important factor affecting the living environment of urban residents. Studying the temporal and spatial variation characteristics of urban anthropogenic heat is of great significance for urban planning and urban ecological service systems. In this study, the urban anthropogenic heat flux (AHF) in 2004, 2009, 2014, and 2020 in the central urban area of Guangzhou was retrieved based on Landsat data and the surface energy balance equation, and the temporal and spatial characteristics of different types of anthropogenic heat were explored by combining the transfer matrix and the migration of the gravity center. The results showed that: (1) The overall change trend of anthropogenic heat in the central urban area of Guangzhou was enhanced, and the degree of enhancement was related to the type of urban functional land. (2) Different types of anthropogenic heat had different characteristics in terms of area expansion and spatial changes. Low-value anthropogenic heat (zero-AHF zone, low-AHF zone, medium-AHF zone) changed drastically in terms of area expansion. High-value anthropogenic heat (medium-AHF zone, high-AHF zone) changed more drastically in space. The increase in urban population, rapid economic development, and increased industrial production activities have stimulated the emission of anthropogenic heat, which has a positive impact on the intensity of anthropogenic heat.
End Point Rate Tool for QGIS (EPR4Q): Validation Using DSAS and AMBUR
Authors: Terres de Lima Lucas , Fernández-Fernández Sandra , Marcel de Almeida Espinoza Jean , da Guia Albuquerque Miguel , Bernardes Cristina
Published: March 2021

This paper presents the validation of the End Point Rate (EPR) tool for QGIS (EPR4Q), a tool built-in QGIS graphical modeler for calculating the shoreline change with the end point rate method. The EPR4Q tries to fill the gaps in user-friendly and free open-source tools for shoreline analysis in a geographic information system environment since the most used software—Digital Shoreline Analysis System (DSAS)—although being a free extension, it is created for commercial software. Additionally, the best free, open-source option to calculate EPR is called Analyzing Moving Boundaries Using R (AMBUR); since it is a robust and powerful tool, the complexity can restrict the accessibility and simple usage. The validation methodology consists of applying the EPR4Q, DSAS, and AMBUR with different types of shorelines found in nature, extracted from the US Geological Survey Open-File. The obtained results of each tool were compared with Pearson’s correlation coefficient. The validation results indicate that the EPR4Q tool acquired high correlation values with DSAS and AMBUR, reaching a coefficient of 0.98 to 1.00 on linear, extensive, and non-extensive shorelines, proving that the EPR4Q tool is ready to be freely used by the academic, scientific, engineering, and coastal managers communities worldwide.
Mapping Invasion Potential of the Pest from Central Asia, Trypophloeus klimeschi (Coleoptera: Curculionidae: Scolytinae), in the Shelter Forests of Northwest China
Authors: Ning Hang , Tang Ming , Chen Hui
Published: March 2021

Temperature and precipitation are the two main factors constraining the current distribution of Trypophloeus klimeschi. Currently, T. klimeschi is mainly distributed in South Xinjiang, where it occurs between the southern edge of the Tianshan Mountains and northern edge of the Tarim Basin. In addition, Dunhuang in northern Gansu also provide suitable habitats for this bark beetle. Two other potential areas for this species are in or near the cities of Alaer and Korla. Under future climate scenarios, its total suitable area is projected to increase markedly over time. Among the climate scenarios, the distribution expanded the most under the maximum greenhouse gas emission scenario (representative concentration pathway (RCP) 8.5). Jiuquan in Gansu is projected to become a suitable area in the 2030s. Subsequently, T. klimeschi is expected to enter western Inner Mongolia along the Hexi Corridor in the 2050s. In southeastern Xinjiang, however, the suitable area in northern Ruoqiang and most areas of Korla may decrease. By the 2050s, it is large enough to pose substantial challenges for forest managers across northern China. Our findings provide information that can be used to monitor T. klimeschi populations, host health, and the impact of climate change, shedding light on the effectiveness of management responses.
Post-Transplant Cyclophosphamide and Tacrolimus—Mycophenolate Mofetil Combination Governs GVHD and Immunosuppression Need, Reducing Late Toxicities in Allogeneic Peripheral Blood Hematopoietic Cell Transplantation from HLA-Matched Donors
Authors: Carnevale-Schianca Fabrizio , Caravelli Daniela , Gallo Susanna , Becco Paolo , Paruzzo Luca , Poletto Stefano , Polo Alessandra , Mangioni Monica , Salierno Milena , Berger Massimo , Pessolano Rosanna , Saglio Francesco , Gottardi Daniela , Rota-Scalabrini Delia , Grignani Giovanni , Fizzotti Marco , Ferrero Ivana , Frascione Pio Manlio Mirko , D’Ambrosio Lorenzo , Gaidano Valentina , Gammaitoni Loretta , Sangiolo Dario , Saglietto Andrea , Vassallo Elena , Cignetti Alessandro , Aglietta Massimo , Fagioli Franca
Published: March 2021

Combined direct antineoplastic activity and the long-lasting immunological effects of allogeneic hematopoietic cell transplant (HCT) can cure many hematological malignancies, but broad adoption requires non-relapse mortality (NRM) rates and graft-versus-host disease (GVHD) control. Recently, posttransplant cyclophosphamide (PTCy) given after a bone marrow transplant significantly reduced GVHD-incidence, while PTCy given with tacrolimus/mofetil mycophenolate (T/MMF) showed activity following allogeneic peripheral blood stem cell transplantation (alloPBSCT). Here, we report the experience of a larger cohort (85 consecutive patients) and expanded follow-up period (03/2011–12/2019) with high-risk hematological malignancies who received alloPBSCT from Human-Leukocyte-Antigens HLA-matched unrelated/related donors. GVHD-prophylaxis was PTCy 50 mg/kg (days+3 and +4) combined with T/MMF (day+5 forward). All patients stopped MMF on day+28 with day+110 = median tacrolimus discontinuation. Cumulative incidences were 12% for acute and 7% for chronic GVHD- and no GVHD-attributed deaths. For surviving patients, the 12, 24, and 36-month probabilities of being off immunosuppression were 92, 96, and 96%, respectively. After a 36-month median follow-up, NRM was 4%; median event-free survival (EFS) and overall survival (OS) had yet to occur. One- and two-year chronic GVHD-EFS results were 57% (95% CI, 46–68%) and 53% (95% CI, 45–61%), respectively, with limited late infections and long-term organ toxicities. Disease relapse caused the most treatment failures (38% at 2 years), but low transplant toxicity allowed many patients (14/37, 38%) to receive donor lymphocyte infusions as a post-relapse strategy. We confirmed that PTCy+T/MMF treatment effectively prevented acute and chronic GVHD and limited NRM to unprecedented low rates without loss of disease control efficacy in an expanded patient cohort. This trial is registered at U.S. National Library of Medicine as #NCT02300571.
Determinants of Outcomes of Acute Kidney Injury: Clinical Predictors and Beyond
Authors: Abdel-Rahman Emaad M. , Turgut Faruk , Gautam Jitendra K. , Gautam Samir C.
Published: March 2021

Acute kidney injury (AKI) is a common clinical syndrome characterized by rapid impairment of kidney function. The incidence of AKI and its severe form AKI requiring dialysis (AKI-D) has been increasing over the years. AKI etiology may be multifactorial and is substantially associated with increased morbidity and mortality. The outcome of AKI-D can vary from partial or complete recovery to transitioning to chronic kidney disease, end stage kidney disease, or even death. Predicting outcomes of patients with AKI is crucial as it may allow clinicians to guide policy regarding adequate management of this problem and offer the best long-term options to their patients in advance. In this manuscript, we will review the current evidence regarding the determinants of AKI outcomes, focusing on AKI-D.
Lack of Association between Postoperative Pancreatitis and Other Postoperative Complications Following Pancreaticoduodenectomy
Authors: Yoo Daegwang , Park Seo Young , Hwang Dae Wook , Lee Jae Hoon , Song Ki Byung , Lee Woohyung , Park Yejong , Jun Eunsung , Kim Song Cheol
Published: March 2021

Background: Prediction of post-pancreaticoduodenectomy (PD) morbidity is difficult, especially in the early postoperative period when CT (Computed Tomography) scans are not available. Elevated serum amylase and lipase in postoperative day 0 or 1 may be used to define postoperative acute pancreatitis (POAP), but the existing literature does not agree on whether POAP is significantly associated with postoperative pancreatic fistula (POPF). Methods: We analyzed the data obtained from a previously published randomized controlled trial. POAP was defined as elevations in serum amylase above 110 U/L on postoperative day 0 or 1. Clinically relevant POAP (CR-POAP) was defined as elevations in C-reactive protein level (CRP) on postoperative day 2 in those with POAP. Postoperative complications including severe complications (Clavien–Dindo ≥ IIIa), POPF, and clinically relevant POPF (CR-POPF) were analyzed. Results: In 246 patients, POAP did not show significant associations with total postoperative complications (odds ratio (OR) 0.697; 95% CI, 0.360–1.313; p = 0.271), severe complications (OR 0.647; 95% CI, 0.258–1.747; p = 0.367), and CR-POPF (OR 0.998; 95% CI, 0.310–3.886; p = 0.998) in multivariable analysis. Conclusions: In patients undergoing PD, POAP was not significantly associated with postoperative complications including POPF. Caution should be taken when using POAP as a predictor of POPF.
The Loop-Mediated Isothermal Amplification Technique in Periodontal Diagnostics: A Systematic Review
Authors: Lenkowski Marcin , Nijakowski Kacper , Kaczmarek Mariusz , Surdacka Anna
Published: March 2021

The course of periodontal disease is affected by many factors; however, the most significant are the dysbiotic microflora, showing different pathogenicity levels. Rapid colonization in the subgingival environment can radically change the clinical state of the periodontium. This systematic review aims to present an innovative technique of loop-mediated isothermal amplification for rapid panel identification of bacteria in periodontal diseases. The decisive advantage of the loop-mediated isothermal amplification (LAMP) technique in relation to molecular methods based on the identification of nucleic acids (such as polymerase chain reaction (PCR or qPCR) is the ability to determine more pathogens simultaneously, as well as with higher sensitivity. In comparison with classical microbiological seeding techniques, the use of the LAMP method shortens a few days waiting time to a few minutes, reducing the time necessary to identify the species and determine the number of microorganisms. The LAMP technology requires only a small hardware base; hence it is possible to use it in outpatient settings. The developed technique provides the possibility of almost immediate assessment of periodontal status and, above all, risk assessment of complications during the treatment (uncontrolled spread of inflammation), which can certainly be of key importance in clinical work.
Ceramic-on-Ceramic Bearing in Total Hip Arthroplasty Reduces the Risk for Revision for Periprosthetic Joint Infection Compared to Ceramic-on-Polyethylene: A Matched Analysis of 118,753 Cementless THA Based on the German Arthroplasty Registry
Authors: Renner Lisa , Perka Carsten , Melsheimer Oliver , Grimberg Alexander , Jansson Volkmar , Steinbrück Arnd
Published: March 2021

Periprosthetic joint infection (PJI) is one of the most common complications in total hip arthroplasty (THA). The influence of bearing material on the risk of PJI remains unclear to date. This registry-based matched study investigates the role of bearing partners in primary cementless THA. Primary cementless THAs recorded in the German Arthroplasty Registry since 2012 with either a ceramic-on-ceramic (CoC) or ceramic-on-polyethylene (CoP) bearings were included in the analysis. Using propensity score matching (PSM) for age, sex, obesity, diabetes mellitus, Elixhauser comorbidity index, year of surgery and head size, we compared the risk for revision for PJI for CoC and CoP. Within the 115,538 THAs (87.1% CoP; 12.9% CoC), 977 revisions were performed due to PJI. There was a significantly higher risk for revision for PJI for CoP compared with CoC over the whole study period (p < 0.01) after 2:1 matching (CoP:CoC) with a hazard ratio of 1.41 (95% confidence interval (CI), 1.09 to 1.80) After 3 years, the risk for revision for PJI was 0.7% (CI 0.5–0.9%) for CoC and 0.9% (CI 0.8–1.1%) for CoP. The risk for revision for all other reasons except PJI did not significantly differ between the two groups over the whole study period (p = 0.4). Cementless THAs with CoC bearings were less likely to be revised because of infection in mid-term follow-up. In the future, registry-embedded studies focusing on long-term follow-up, including clinical data, as well as basic science studies, may give a deeper insight into the influence of the bearing partners.
The Evolution of Living Donor Nephrectomy Program at A Hellenic Transplant Center. Laparoscopic vs. Open Donor Nephrectomy: Single-Center Experience
Authors: Vernadakis Spyridon , Marinaki Smaragdi , Darema Maria , Soukouli Ioanna , Michelakis Ioannis El. , Beletsioti Chrysoula , Zavvos Georgios , Bokos Ioannis , Boletis Ioannis N.
Published: March 2021

Since its introduction in 1995, laparoscopic nephrectomy has emerged as the preferred surgical approach for living donor nephrectomy. Given the ubiquity of the surgical procedure and the need for favorable outcomes, as it is an elective operation on otherwise healthy individuals, it is imperative to ensure appropriate preoperative risk stratification and anticipate intraoperative challenges. The aim of the present study was to compare peri-and postoperative outcomes of living kidney donors (LD), who had undergone laparoscopic nephrectomy (LDN), with a control group of those who had undergone open nephrectomy (ODN). Health-related quality of life (QoL) was also assessed using the validated SF-36 questionnaire. Data from 252 LD from a single transplant center from March 2015 to December 2020 were analyzed retrospectively. In total, 117 donors in the LDN and 135 in the ODN groups were assessed. Demographics, type of transplantation, BMI, duration of surgery, length of hospital stay, peri- and postoperative complications, renal function at discharge and QoL were recorded and compared between the two groups using Stata 13.0 software. There was no difference in baseline characteristics, nor in the prevalence of peri-and postoperative complications, with a total complication rate of 16% (mostly minor, Clavien–Dindo grade II) in both groups, while a different pattern of surgical complications was noticed between them. Duration of surgery was significantly longer in the ODN group (median 240 min vs. 160 min in LDN, p < 0.01), warm ischemia time was longer in the LDN group (median 6 min vs.2 min in ODN, p < 0.01) and length of hospital stay shorter in the LDN group (median 3 days vs. 7 days in ODN). Conversion rate from laparoscopic to open surgery was 2.5%. There was a drop in estimated glomerular filtration rate (eGFR) at discharge of 36 mL/min in the LDN and 32 mL/min in the ODN groups, respectively (p = 0.03). No death, readmission or reoperation were recorded. There was a significant difference in favor of LDN group for each one of the eight items of the questionnaire (SF1–SF8). As for the two summary scores, while the total physical component summary (PCS) score was comparable between the two groups (57.87 in the LDN group and 57.07 in the ODN group), the mental component summary (MCS) score was significantly higher (62.14 vs. 45.22, p < 0.001) in the LDN group. This study provides evidence that minimally invasive surgery can be performed safely, with very good short-term outcomes, providing several benefits for the living kidney donor, thereby contributing to expanding the living donor pool, which is essential, especially in countries with deceased-donor organ shortage.
Could Subtle Obstetrical Brachial Plexus Palsy Be Related to Unilateral B Glenoid Osteoarthritis?
Authors: Lädermann Alexandre , Bothorel Hugo , Collin Philippe , Elhassan Bassem , Favard Luc , Bernal Nazira , Raiss Patric , Athwal George S.
Published: March 2021

Background: Several factors associated with B glenoid are also linked with obstetrical brachial plexus palsy (OBPP). The purpose of this observational study was to determine the incidence of OBPP risk factors in type B patients. Methods: A cohort of 154 patients (68% men, 187 shoulders) aged 63 ± 17 years with type B glenoids completed a questionnaire comprising history of perinatal characteristics related to OBPP. A literature review was performed following the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) to estimate the incidence of OBPP risk factors in the general population. Results: Twenty-seven patients (18%) reported one or more perinatal OBPP risk factors, including shoulder dystocia (n = 4, 2.6%), macrosomia >4 kg (n = 5, 3.2%), breech delivery (n = 6, 3.9%), fetal distress (n = 8, 5.2%), maternal diabetes (n = 2, 1.3%), clavicular fracture (n = 2, 1.3%), and forceps delivery (n = 4, 2.6%). The comparison with the recent literature suggested that most perinatal OBPP risk factors were within the normal range, although the incidence of shoulder dystocia, forceps and vaginal breech deliveries exceeded the average rates. Conclusion: Perinatal factors related to OBPP did not occur in a higher frequency in patients with Walch type B OA compared to the general population, although some of them were in the high normal range.
The Neuroprotective Effect of Thiopental on the Postoperative Neurological Complications in Patients Undergoing Surgical Clipping of Unruptured Intracranial Aneurysm: A Retrospective Analysis
Authors: Kim Byung-Gun , Jeon Young-Tae , Han Jiwon , Bae Yu Kyung , Lee Si Un , Ryu Jung-Hee , Koo Chang-Hoon
Published: March 2021

Although thiopental improved neurological outcomes in several animal studies, there are still insufficient clinical data examining the efficacy of thiopental for patients undergoing surgical clipping of unruptured intracranial aneurysm (UIA). This study validated the effect of thiopental and investigated risk factors associated with postoperative neurological complications in patients undergoing surgical clipping of UIA. In total, 491 patients who underwent aneurysm clipping were included in this retrospective cohort study. Data regarding demographics, aneurysm characteristics, and use of thiopental were collected from electronic medical records. Propensity score matching and logistic regression analysis were used. After propensity score matching, the thiopental group showed a lower incidence of the postoperative neurological complications than non-thiopental group (5.5% vs. 17.1%, p = 0.001). In multivariate analysis, thiopental reduced the risk of postoperative neurological complications (odds ratio (OR) 0.26, 95% confidence interval (CI) 0.13 to 0.51, p < 0.001) while aneurysm size ≥ 10 mm (OR 4.48, 95% CI 1.69 to 11.87, p = 0.003), and hyperlipidemia (OR 2.24, 95% CI 1.16 to 4.32, p = 0.02) increased the risk of postoperative neurological complications. This study showed that thiopental was associated with the lower risk of neurological complications after clipping of UIA.
Protect the Natives to Combat the Aliens: Could Octopus vulgaris Cuvier, 1797 Be a Natural Agent for the Control of the Lionfish Invasion in the Mediterranean Sea?
Authors: Crocetta Fabio , Shokouros-Oskarsson Maria , Doumpas Nikolaos , Giovos Ioannis , Kalogirou Stefanos , Langeneck Joachim , Tanduo Valentina , Tiralongo Francesco , Virgili Riccardo , Kleitou Periklis
Published: March 2021

Biological invasions constitute a major threat to native ecosystems and to global biodiversity [...]
Data-Driven Math Model of FLT3-ITD Acute Myeloid Leukemia Reveals Potential Therapeutic Targets
Authors: Wooten David J. , Gebru Melat , Wang Hong-Gang , Albert Réka
Published: March 2021

FLT3-mutant acute myeloid leukemia (AML) is an aggressive form of leukemia with poor prognosis. Treatment with FLT3 inhibitors frequently produces a clinical response, but the disease nevertheless often recurs. Recent studies have revealed system-wide gene expression changes in FLT3-mutant AML cell lines in response to drug treatment. Here we sought a systems-level understanding of how these cells mediate these drug-induced changes. Using RNAseq data from AML cells with an internal tandem duplication FLT3 mutation (FLT3-ITD) under six drug treatment conditions including quizartinib and dexamethasone, we identified seven distinct gene programs representing diverse biological processes involved in AML drug-induced changes. Based on the literature knowledge about genes from these modules, along with public gene regulatory network databases, we constructed a network of FLT3-ITD AML. Applying the BooleaBayes algorithm to this network and the RNAseq data, we created a probabilistic, data-driven dynamical model of acquired resistance to these drugs. Analysis of this model reveals several interventions that may disrupt targeted parts of the system-wide drug response. We anticipate co-targeting these points may result in synergistic treatments that can overcome resistance and prevent eventual recurrence.
The 3′-UTR Polymorphisms in the Thymidylate Synthase (TS) Gene Associated with the Risk of Ischemic Stroke and Silent Brain Infarction
Authors: Kim Jung Oh , Park Han Sung , Ko Eun Ju , Sung Jung Hoon , Kim Jinkwon , Oh Seung Hun , Kim Ok Joon , Kim Nam Keun
Published: March 2021

Thymidylate synthase (TS) is a key gene involved in the repair of DNA damage and DNA synthesis that plays an important role in vascular development and recovery. In particular, TS gene polymorphisms play a major role in the progression of vascular disease and cancer metastasis. Therefore, the aim of this study was to investigate the association of three TS polymorphisms (1100T>C [rs699517], 1170A>G [rs2790], and 1494ins/del [rs151264360]) with ischemic stroke and silent brain infarction (SBI) in Koreans. A total of 1299 participants (507 stroke patients, 383 SBI patients, and 409 controls) were enrolled in the study. Genotyping of the three TS polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism analysis. To examine the association between TS gene polymorphisms and the diseases, we performed statistical analyses, including multivariable logistic regression and Fisher’s exact tests. We found that TS 1100T>C and 1170A>G genotypes were strongly associated with ischemic stroke and SBI susceptibility. More specifically, the TS 1100T>C polymorphism was associated with the likelihood of ischemic stroke (TT vs. CC: AOR = 2.151, 95% CI = 1.275–3.628, P = 0.004) and SBI (TT vs. TC+CC: AOR = 1.443, 95 % CI = 1.009–2.063, P = 0.045). In contrast, the TS 1170A > G polymorphism exhibited lower correlation with the risk of stroke (AA vs. GG: AOR = 0.284, 95% CI = 0.151–0.537, P < 0.0001) and SBI (AA vs. GG: AOR = 0.070, 95% CI = 0.016–0.298, P = 0.0002). Furthermore, we confirmed that the TS 1100T>C polymorphism was synergistic with low folic acid levels (AOR = 6.749, P < 0.0001). Altogether, these results suggest that TS 1100T>C and 1170A > G polymorphisms are associated with the risk of ischemic stroke and SBI, and our study provides the first evidence that 3′-UTR variants in TS are potential biomarkers in ischemic stroke and SBI.
Relationships between Morphostructural/Geological Framework and Landslide Types: Historical Landslides in the Hilly Piedmont Area of Abruzzo Region (Central Italy)
Authors: Esposito Gianluca , Carabella Cristiano , Paglia Giorgio , Miccadei Enrico
Published: March 2021

Landslides are a widespread natural phenomenon that play an important role in landscape evolution and are responsible for several casualties and damages. The Abruzzo Region (Central Italy) is largely affected by different types of landslides from mountainous to coastal areas. In particular, the hilly piedmont area is characterized by active geomorphological processes, mostly represented by slope instabilities related to mechanisms and factors that control their evolution in different physiographic and geological–structural conditions. This paper focuses on the detailed analysis of three selected case studies to highlight the multitemporal geomorphological evolution of landslide phenomena. An analysis of historical landslides was performed through an integrated approach combining literature data and landslide inventory analysis, relationships between landslide types and lithological units, detailed photogeological analysis, and geomorphological field mapping. This analysis highlights the role of morphostructural features on landslide occurrence and distribution and their interplay with the geomorphological evolution. This work gives a contribution to the location, abundance, activity, and frequency of landslides for the understanding of the spatial interrelationship of landslide types, morphostructural setting, and climate regime in the study area. Finally, it represents a scientific tool in geomorphological studies for landslide hazard assessment at different spatial scales, readily available to interested stakeholders to support sustainable territorial planning.
Soil Diversity (Pedodiversity) and Ecosystem Services
Authors: Mikhailova Elena A. , Zurqani Hamdi A. , Post Christopher J. , Schlautman Mark A. , Post Gregory C.
Published: March 2021

Soil ecosystem services (ES) (e.g., provisioning, regulation/maintenance, and cultural) and ecosystem disservices (ED) are dependent on soil diversity/pedodiversity (variability of soils), which needs to be accounted for in the economic analysis and business decision-making. The concept of pedodiversity (biotic + abiotic) is highly complex and can be broadly interpreted because it is formed from the interaction of atmospheric diversity (abiotic + biotic), biodiversity (biotic), hydrodiversity (abiotic + biotic), and lithodiversity (abiotic) within ecosphere and anthroposphere. Pedodiversity is influenced by intrinsic (within the soil) and extrinsic (outside soil) factors, which are also relevant to ES/ED. Pedodiversity concepts and measures may need to be adapted to the ES framework and business applications. Currently, there are four main approaches to analyze pedodiversity: taxonomic (diversity of soil classes), genetic (diversity of genetic horizons), parametric (diversity of soil properties), and functional (soil behavior under different uses). The objective of this article is to illustrate the application of pedodiversity concepts and measures to value ES/ED with examples based on the contiguous United States (U.S.), its administrative units, and the systems of soil classification (e.g., U.S. Department of Agriculture (USDA) Soil Taxonomy, Soil Survey Geographic (SSURGO) Database). This study is based on a combination of original research and literature review examples. Taxonomic pedodiversity in the contiguous U.S. exhibits high soil diversity, with 11 soil orders, 65 suborders, 317 great groups, 2026 subgroups, and 19,602 series. The ranking of “soil order abundance” (area of each soil order within the U.S.) expressed as the proportion of the total area is: (1) Mollisols (27%), (2) Alfisols (17%), (3) Entisols (14%), (4) Inceptisols and Aridisols (11% each), (5) Spodosols (3%), (6) Vertisols (2%), and (7) Histosols and Andisols (1% each). Taxonomic, genetic, parametric, and functional pedodiversity are an essential context for analyzing, interpreting, and reporting ES/ED within the ES framework. Although each approach can be used separately, three of these approaches (genetic, parametric, and functional) fall within the “umbrella” of taxonomic pedodiversity, which separates soils based on properties important to potential use. Extrinsic factors play a major role in pedodiversity and should be accounted for in ES/ED valuation based on various databases (e.g., National Atmospheric Deposition Program (NADP) databases). Pedodiversity is crucial in identifying soil capacity (pedocapacity) and “hotspots” of ES/ED as part of business decision making to provide more sustainable use of soil resources. Pedodiversity is not a static construct but is highly dynamic, and various human activities (e.g., agriculture, urbanization) can lead to soil degradation and even soil extinction.
Arthritogenic Alphavirus Capsid Protein
Authors: Rao Shambhavi , Taylor Adam
Published: March 2021

In the past two decades Old World and arthritogenic alphavirus have been responsible for epidemics of polyarthritis, causing high morbidity and becoming a major public health concern. The multifunctional arthritogenic alphavirus capsid protein is crucial for viral infection. Capsid protein has roles in genome encapsulation, budding and virion assembly. Its role in multiple infection processes makes capsid protein an attractive target to exploit in combating alphaviral infection. In this review, we summarize the function of arthritogenic alphavirus capsid protein, and describe studies that have used capsid protein to develop novel arthritogenic alphavirus therapeutic and diagnostic strategies.
The State of the Art and Prospects for Osteoimmunomodulatory Biomaterials
Authors: Negrescu Andreea-Mariana , Cimpean Anisoara
Published: March 2021

The critical role of the immune system in host defense against foreign bodies and pathogens has been long recognized. With the introduction of a new field of research called osteoimmunology, the crosstalk between the immune and bone-forming cells has been studied more thoroughly, leading to the conclusion that the two systems are intimately connected through various cytokines, signaling molecules, transcription factors and receptors. The host immune reaction triggered by biomaterial implantation determines the in vivo fate of the implant, either in new bone formation or in fibrous tissue encapsulation. The traditional biomaterial design consisted in fabricating inert biomaterials capable of stimulating osteogenesis; however, inconsistencies between the in vitro and in vivo results were reported. This led to a shift in the development of biomaterials towards implants with osteoimmunomodulatory properties. By endowing the orthopedic biomaterials with favorable osteoimmunomodulatory properties, a desired immune response can be triggered in order to obtain a proper bone regeneration process. In this context, various approaches, such as the modification of chemical/structural characteristics or the incorporation of bioactive molecules, have been employed in order to modulate the crosstalk with the immune cells. The current review provides an overview of recent developments in such applied strategies.
Effect of a Multiple Reduction Die on the Residual Stress of Drawn Materials
Authors: Kim Jeong-Hun , Baek Chang-Hyun , Lee Sang-Kon , Kang Jong-Hun , Park Joon-Hong , Ko Dae-Cheol
Published: March 2021

Residual stress may influence the mechanical behavior and durability of drawn materials. Thus, this study develops a multiple reduction die (MRD) that can reduce residual stress during the drawing process. The MRD set consists of several die tips, die cases, and lubricating equipment. All the die tips of the MRD were disposed of simultaneously. Finite element analysis of the drawing process was performed according to the reduction ratio of each die tip, and the variables in drawing process with the MRD were optimized using a deep neural network to minimize the residual stress. Experiments on the drawing process with the conventional die and MRD were performed to evaluate the residual stress and verify the effectiveness of the MRD. The results of X-ray diffraction measurements indicated that the axial and hoop residual stresses on the surface were dramatically reduced.
Weldability of Underwater Wet-Welded HSLA Steel: Effects of Electrode Hydrophobic Coatings
Authors: Tomków Jacek
Published: March 2021

The paper presents the effects of waterproof coatings use to cover electrodes on the weldability of high-strength, low-alloy (HSLA) steel in water. With the aim of improving the weldability of S460N HSLA steel in water, modifications of welding filler material were chosen. The surfaces of electrodes were covered by different hydrophobic substances. The aim of the controlled thermal severity (CTS) test was to check the influence of these substances on the HSLA steel weldability in the wet welding conditions. The visual test, metallographic tests, and hardness Vickers HV10 measurements were performed during investigations. The results proved that hydrophobic coatings can reduce the hardness of welded joints in the heat-affected zone by 40–50 HV10. Additionally, the number of cold cracks can be significantly reduced by application of waterproof coatings on the filler material. The obtained results showed that electrode hydrophobic coatings can be used to improve the weldability of HSLA steel in underwater conditions.
Closed-Surface Multifunctional Antireflective Coating Made from SiO2 with TiO2 Nanocomposites
Authors: Guo Zhiqiu , Zhu Ze , Liu Ya , Wu Changjun , Tu Hao , Wang Jianhua , Su Xuping
Published: March 2021

An SiO2-TiO2 closed-surface antireflective coating was fabricated by the one-dipping method. TiO2 nanoparticles were mixed with a nanocomposited silica sol, which was composed of acid-catalyzed nanosilica networks and silica hollow nanospheres (HNs). The microstructure of the sol-gel was characterized by transmission electron microscopy. The silica HNs were approximately 40–50 nm in diameter with a shell thickness of approximately 8–10 nm. The branched-chain structure resulting from acidic hydrolysis grew on these silica HNs, and TiO2 was distributed inside this network. The surface morphology of the coating was measured by field emission scanning electron microscopy and atomic force microscopy. After optimization, transmittance of up to 94.03% was obtained on photovoltaic (PV) glass with a single side coated by this antireflective coating, whose refractive index was around 1.30. The short-circuit current gain of PV module was around 2.14–2.32%, as shown by the current-voltage (IV) curve measurements and external quantum efficiency (EQE) tests. This thin film also exhibited high photocatalytic activity. Due to the lack of voids on its surface, the antireflective coating in this study possessed excellent long-term reliability and robustness in both high-moisture and high-temperature environments. Combined with its self-cleaning function, this antireflective coating has great potential to be implemented in windows and photovoltaic modules.
Electropolymerised Polypyrroles as Active Layers for Molecularly Imprinted Sensors: Fabrication and Applications
Authors: Glosz Karolina , Stolarczyk Agnieszka , Jarosz Tomasz
Published: March 2021

Conjugated polymers are widely used in the development of sensors, but even though they are sensitive and robust, they typically show limited selectivity, being cross-sensitive to many substances. In turn, molecular imprinting is a method involving modification of the microstructure of the surface to incorporate cavities, whose shape matches that of the “template”—the analyte to be detected, resulting in high selectivity. The primary goal of this review is to report on and briefly explain the most relevant recent developments related to sensors utilising molecularly imprinted polypyrrole layers and their applications, particularly regarding the detection of bioactive substances. The key approaches to depositing such layers and the most relevant types of analytes are highlighted, and the various trends in the development of this type of sensors are explored.
Use of PROMETHEE MCDA Method for Ranking Alternative Measures of Sustainable Urban Mobility Planning
Authors: Morfoulaki Maria , Papathanasiou Jason
Published: March 2021

One of the most crucial steps during the implementation of a Sustainable Urban Mobility Plan (SUMP) as well the relevant transportation projects is the final measures and policies’ selection that will be realized in order for the plan to achieve the study area targets. There are many methodologies that have been used for a specific purpose, with cost-effective and cost–benefit analysis being the most popular. According to the new specifications of SUMPs, the co-creation and co-planning of the future measures taking into account the opinions of all the relevant stakeholders and groups of citizens is the main parameter that will ensure the success of the planning. For this reason, MCA is the methodology proposed to be used for collecting and analyzing the different opinions. The aim of the current work is to prove, through a targeted Greek survey, the effectiveness of the MCA, not only to merge the different opinions and priorities of the stakeholders but also to highlight and rank realistically the most important sustainable mobility measures that should be implemented in an urban area. The work proposes and tests a specific methodological framework based on the use of the preference ranking organization method for enrichment of evaluations (PROMETHEE) method.