bluuebunny/crossref_metadata_2025 huggingface dataset:
An Alternative Approach for the Design of Chevron-Braced Frames
Authors: Barbagallo Francesca , Bosco Melina , Caragliano Marco , Marino Edoardo M. , Rossi Pier Paolo
Published: November 2021

The design of steel chevron-braced frames as per Eurocode 8 is based on the idea that only the braces should buckle and yield during ground motions, while other members should remain elastic. The elastic design of the braced frames is also allowed. However, in both cases, the seismic performance of the frame may be compromised because of premature yielding/buckling of columns. This paper proposes an alternative design procedure that promotes yielding of beams in addition to yielding of braces. This mitigates the vertical unbalanced force transmitted by compressive and tensile braces to the beam and in turn reduces the internal forces of the columns. The result is the overall improvement of the seismic performance owing to the reduction of the number of cases in which failure of the columns occurs before full exploitation of the ductility capacity of the dissipative members. The proposed design procedure is validated by incremental dynamic analyses performed on a set of chevron-braced frames. In particular, the peak ground accelerations of the frames designed by the proposed procedure at the attainment of Significant Damage and Collapse Prevention limit states are determined and compared to those of frames designed according to Eurocode 8. Furthermore, frames designed according to the Eurocodes and to the proposed method are compared in terms of structural cost.
Generation of a Panorama Compatible with the JPEG 360 International Standard Using a Single PTZ Camera
Authors: Ullah Faiz , Kwon Oh-Jin , Choi Seungcheol
Published: November 2021

Recently, the JPEG working group (ISO/IEC JTC1 SC29 WG1) developed an international standard, JPEG 360, that specifies the metadata and functionalities for saving and sharing 360-degree images efficiently to create a more realistic environment in various virtual reality services. We surveyed the metadata formats of existing 360-degree images and compared them to the JPEG 360 metadata format. We found that existing omnidirectional cameras and stitching software packages use formats that are incompatible with the JPEG 360 standard to embed metadata in JPEG image files. This paper proposes an easy-to-use tool for embedding JPEG 360 standard metadata for 360-degree images in JPEG image files using a JPEG-defined box format: the JPEG universal metadata box format. The proposed implementation will help 360-degree cameras and software vendors provide immersive services to users in a standardized manner for various markets, such as entertainment, education, professional training, navigation, and virtual and augmented reality applications. We also propose and develop an economical JPEG 360 standard compatible panoramic image acquisition system from a single PTZ camera with a special-use case of a wide field of view image of a conference or meeting. A remote attendee of the conference/meeting can see the realistic and immersive environment through our PTZ panorama in virtual reality.
Mechanism of and Key Technologies for Copper Bonding in the Hot Rolling of SCR Continuous Casting and Rolling
Authors: Liu Yang , Peng Yan , Qu Xiaobo
Published: November 2021

In the process of copper alloy hot continuous rolling, the problem of copper sticking to the roller seriously affects the surface quality, performance, and service life of the copper products. Roll sticking occurs as the adhesion energy of Cu is lower than that of Fe and the Fe-Cu interface, and the severe surface deformation which forces the copper into direct contact with the roll during the process of profile rolling. Based on the copper deformation law and adhesion phenomenon in the hot continuous rolling process, a rolling deformation model and roll copper adhesion model or copper alloy hot continuous rolling were established, and their simulation was realized using finite element software. Through finite element modeling of the hot rolling deformation zone, the distribution of the temperature, contact normal stress, and exposure rate in the hot rolling deformation zone were obtained, which were consistent with the actual roll adhesion phenomenon and copper adhesion position. To address the copper sticking behavior of the rolls, the process optimization method of matching the motor speed with the elongation coefficient (the 1# and 2# motor speeds were adjusted to 1549 r/min and 1586 r/min, respectively), adjusting the roll gap to 7.9 mm, and increasing the number and pressure of roll spray nozzles were put forward, which effectively solved the problem of copper sticking to the roll, significantly improved the surface quality of the copper and the service life of the roll, and can be used in production.
Effects of Incorporation of Marine Derived Hydroxyapatite on the Microhardness, Surface Roughness, and Fluoride Release of Two Glass-Ionomer Cements
Authors: Bilić-Prcić Maja , Šalinović Ivan , Gurgan Sevil , Koc Vural Uzay , Krmek Silvana Jukić , Miletić Ivana
Published: November 2021

Background: The aim of this study was to evaluate the effects of incorporation of hydroxyapatite (HA) derived from cuttlefish bone on the microhardness, surface roughness (SR), and fluoride release (FR) of conventional cure, and resin-modified glass-ionomer cement. Methods: There were four groups for each tested material; experimental glass-ionomer were made by addition and of 2, 5, and 10 wt % HA respectively to conventional glass-ionomers Fuji II LC and Fuji IX GP Extra. One group was prepared without the addition of HA particles. For SR and microhardness measurements sectional Teflon molds (5 mm in diameter and 2 mm deep) were used to prepare 10 samples per group (n = 80). The samples were stored in distilled water at 37 °C for 7 days prior to testing. The SR was measured using a contact type profilometer and the microhardness was determined using a Vickers micro-hardness tester at a load of 980 g for 15 s. For FR measurements, there were six samples per group (n = 48), prepared in Teflon molds (8 mm in diameter and 2 mm deep). The FR was measured with an ionoselective electrode in triplicates after 24 h, 7 days, and 45 days. Statistical analysis was performed using one-way ANOVA with Tukey post-hoc test. Results and Conclusion: Microhardness values obtained for Fuji II modified with 10 wt % HA were significantly higher compared to the other two groups tested. Comparison of materials with respect to SR showed significant difference between them (p < 0.0001) with Fuji II and Fuji IX modified with HA having higher SR values. Regarding FR, Fuji IX showed statistically significant higher results than Fuji II, independently of HA modification, and groups modified with 2 and 5 wt % HA showed significantly increased fluoride release in all three time points.
Analysis of Symptomology, Infectiveness, and Reinfections between Male and Female COVID-19 Patients: Evidence from Japanese Registry Data
Authors: Li Meng-Hao , Siddique Abu Bakkar , Andalibi Ali , Koizumi Naoru
Published: November 2021

Background: Hokkaido was the first Japanese prefecture to be affected by COVID-19. Since the beginning of the pandemic, the Japanese government has been publishing the information of each individual who was tested positive for the virus. Method: The current study analyzed the 1269 SARS-CoV-2 cases confirmed in Hokkaido in order to examine sex-based differences in symptomology and infectiveness, as well as the status of reinfections and the viral transmission networks. Results: The majority of asymptomatic patients were females and older. Females were 1.3-fold more likely to be asymptomatic (p < 0.001) while a decade of difference in age increased the likelihood of being asymptomatic by 1% (p < 0.001). The data contained information up to quaternary viral transmission. The transmission network revealed that, although asymptomatic patients are more likely to transmit the virus, the individuals infected by asymptomatic cases are likely to be asymptomatic (p < 0.001). Four distinct co-occurrences of symptoms were observed, including (i) fever/fatigue, (ii) pharyngitis/rhinitis, (iii) ageusia/anosmia, and (iv) nausea/vomiting/diarrhea. The presences of diarrhea (p = 0.05) as well as nausea/vomiting (p < 0.001) were predictive of developing dyspnea, i.e., severe disease. About 1% of the patients experienced reinfection. Conclusions: Sex and symptomatology appear to play important roles in determining the levels of viral transmission as well as disease severity.
VOCs Fugitive Emission Characteristics and Health Risk Assessment from Typical Plywood Industry in the Yangtze River Delta Region, China
Authors: Hu Kun , Liu Zhiqiang , Wang Ming , Zhang Bingjie , Lin Haotian , Lu Xingdong , Chen Wentai
Published: November 2021

Volatile organic compounds (VOCs) emissions from the plywood manufacturing industry in China have received concerns during recent years. A total of 115 VOCs were measured in the adhesive-making, adhesive-coating, and hot-pressing workshops of the plywood manufacturing industry to investigate fugitive emission characteristics of VOCs and assess their health risks to workers. The average concentration of total VOCs in workshops of the plywood manufacturing industry is 467 ± 359 μg/m3, whereas the value for ambient air is 81.4 μg/m3. For specific processes, the adhesive-coating and hot-pressing processes show higher VOCs concentrations (501 μg/m3–519 μg/m3) than the adhesive-making process (340 μg/m3). Formaldehyde, ethyl acetate, and dichloromethane are the three most abundant VOCs in workshops, with relative contributions to total VOCs of 55.9–63.1%, 4.3–11.0%, and 1.7–4.4%, respectively. For ozone formation potential (OFP) of VOCs, formaldehyde is the largest contributor (86.1%), followed by toluene, xylenes, and propanal. The non-cancer toxic risks (HI) and cancer risks of total VOCs (T-LCR) for three processes are calculated as 2.93–3.94 and 2.86–4.17 × 10−4 using the US EPA recommended methods, both significantly higher than threshold values (1.0 for HI and 10−4 for LCR), suggesting the highly toxic and cancer risks to workers. Formaldehyde contributes 68.1–78.2% and 91.4–93.9% of HI and T-LCR, respectively. The designed risk reduction scheme of VOCs based on air ventilation suggests that air ventilation rates of formaldehyde need to reach 4–5 times in 8 h in three processes to reduce T-LCR to 10−5. These results are useful for developing VOCs control measures and evaluating VOCs occupational health risk for workers in the plywood manufacturing industry.
Estimation of Emission Factors for Hazardous Air Pollutants from Petroleum Refineries
Authors: Polvara Elisa , Roveda Luca , Invernizzi Marzio , Capelli Laura , Sironi Selena
Published: November 2021

The hazardous air pollutants (HAPs) group is composed of 187 chemicals that are known to be potentially carcinogenic and dangerous for human health. Due to their toxicological impact, HAPs are an increasingly studied class of compounds. Of the different HAPs sources, refineries are one of the major sources. In order to obtain a preliminary assessment of the impact of a refinery in terms of emissions, a useful instrument is the determination of the emission factor (EF). For this reason, this work, focusing on the USA refining scenario, aims to provide evidence for a generic trend in refinery emissions to evaluate a correlation between the plant size and the amount of its emissions, in particular the HAPs emissions. Based on the analysis of the data collected from the U.S. Environmental Protection Agency (US EPA), a general trend in the emissions from refinery plants was established, showing a positive correlation between the HAPs emissions and the refinery size, represented by a value of the Pearson correlation coefficient r close to 1. Once this correlation was highlighted, a purpose of this work became the estimation of an organic HAPs emission factor (EF): from a whole refining plant, the EF of the total organic HAPs is equal to 10 g emitted for each ton of crude oil processed. Moreover, it was also possible to undertake the same evaluation for two specific HAP molecules: benzene and formaldehyde. The benzene and formaldehyde EFs are equal to, respectively, 0.8 g and 0.2 g for each ton of processed crude oil. This work provides a simple rule of thumb for the estimation of hazardous substances emitted from petroleum refineries in their mean operating conditions.
Assessing Health Impacts of Winter Smog in Lahore for Exposed Occupational Groups
Authors: Jabeen Fatima , Ali Zulfiqar , Maharjan Amina
Published: November 2021

The goal of this research was to investigate the health effects of winter pollution on various occupations in Lahore and its neighboring peri-urban areas. A questionnaire survey, key informants, and focused group discussions were employed to collect data, which included demographic, socioeconomic, and health-related information. Descriptive statistics and the multivariate logistic regression model (MLRM) were used to examine the effects of pollution on exposed occupational groups who experienced symptoms such as coughing, shortness of breath, and eye discomfort. According to data from interviews, MLRM revealed that individuals working in various occupations with outdoor and indoor environments are equally affected by winter smog, but being middle-aged (odds ratio OR = 5.73), having a history of a respiratory ailment (OR = 4.06), and location (OR = 2.26) all play important roles in determining health. However, less educated people, elders, and people who already live in polluted areas are more likely to develop respiratory health symptoms. During the smog incident, it was determined that diverse health and socioeconomic factors exacerbate an individual’s negative health impact more than others.
Study on Icing Environment Judgment Based on Radar Data
Authors: Wang Jinhu , Xie Binze , Cai Jiahan , Wang Yuhao , Chen Jiang
Published: November 2021

As a major threat to aviation flight safety, it is particularly important to make accurate judgments and forecasts of the ice accumulation environment. Radar is widely used in civil aviation and meteorology, and has the advantages of high timeliness and resolution. In this paper, a variety of machine learning methods are used to establish the relationship between radar data and icing index (Ic) to determine the ice accumulation environment. The research shows the following. (1) A linear model was established, based on the scattering rate factor (Zh), radial velocity (v), spectral width (w), velocity standard deviation (σ) detected by 94 GHz millimeter wave radar, and backward attenuation coefficient (β) detected by 905 nm lidar, so linear regression was carried out. After principal component analysis (PCA), the correction determination coefficient of the linear equation was increased from 0.7127 to 0.7240. (2) Ice accumulation was unlikely for samples that were significantly off-center. By clustering the data into three or four categories, the proportion of icing lattice points could be increased from 18.81% to 33.03%. If the clustering number was further increased, the ice accumulation ratio will not be further increased, and the increased classification is reflected in the classification of pairs of noises and the possibility of omission is also increased. (3) Considering the classification and nonlinear factors of ice accumulation risk, the neural network method was used to judge the ice accumulation environment. Two kinds of neural network structures were established for quantitative calculation: Structure 1 first distinguished whether there was ice accumulation, and further calculated the icing index for the points where there was ice accumulation; Structure 2 directly calculated the temperature and relative humidity, and calculated the icing index according to definition. The accuracy of the above two structures could reach nearly 60%, but the quantitative judgment of the ice accumulation index was not ideal. The reasons for this dissatisfaction may be the small number of variables and samples, the interval between time and space, the difference in instrument detection principle, and the representativeness of the ice accumulation index. Further research can be improved from the above four points. This study can provide a theoretical basis for the diagnosis and analysis of the aircraft ice accumulation environment.
An Object-Based Method for Tracking Convective Storms in Convection Allowing Models
Authors: Han Fan , Wang Xuguang
Published: November 2021

The steady-state assumption commonly used in object-based tracking algorithms may be insufficient to determine the right track when a convective storm goes through a complicated evolution. Such an issue is exacerbated by the relatively coarse output frequency of current convection allowing model (CAM) forecasts (e.g., hourly), giving rise to many spatially well resolved but temporally not well resolved storms that steady-state assumption could not account for. To reliably track simulated storms in CAM outputs, this study proposed an object-based method with two new features. First, the method explicitly estimated the probability of each probable track based on either its immediate past and future motion or a reliable “first-guess motion” derived from storm climatology or near-storm environmental variables. Second, object size was incorporated into the method to help identify temporally not well resolved storms and minimize false tracks derived for them. Parameters of the new features were independently derived from a storm evolution analysis using 2-min Multi-Radar Multi-Sensor (MRMS) data and hourly CAM forecasts produced by the University of Oklahoma (OU) Multiscale data Assimilation and Predictability Laboratory (MAP) from May 2019. The performance of the new method was demonstrated with hourly MRMS and CAM forecast examples from May 2018. A systematic evaluation of four severe weather events indicated 99% accuracy achieved for over 600 hourly MRMS tracks derived with the proposed tracking method.
Precipitation in the Czech Republic in Light of Subjective and Objective Classifications of Circulation Types
Authors: Řehoř Jan , Brázdil Rudolf , Lhotka Ondřej , Trnka Miroslav , Balek Jan , Štěpánek Petr , Zahradníček Pavel
Published: November 2021

Many studies in Europe have investigated the relationship between climatological variables and circulation patterns expressed by various classifications of circulation types. This study provides new insights based on an analysis of precipitation in the western (Bohemia—BOH) and eastern (Moravia and Silesia—M&S) parts of the Czech Republic with respect to the subjective classification of the Czech Hydrometeorological Institute and objective classification based on the flow strength, flow direction, and vorticity during the 1961–2020 period. Circulation types are investigated in regard to their contributions to the total precipitation, mean daily precipitation totals, and precipitation probability (daily totals ≥ 1.0 mm). Types with a westerly airflow and a trough over Central Europe exhibit the highest proportions in precipitation totals. Types with a cyclone over Central Europe, especially combined with a northwestern (BOH) or northeastern (M&S) airflow, result in the highest daily mean totals and precipitation probability. Types with a southwestern airflow transport more precipitation to BOH, while those with a northeastern airflow transport more precipitation to M&S, with a slight seasonal shift in the gradient axis between winter and summer. Circulation types under both classifications are examined from the perspective of their precipitation representation in BOH and M&S and the differences between these two regions. In addition, the suitability of both classifications for precipitation analysis is investigated.
Engineering Breast Cancer Cells and hUMSCs Microenvironment in 2D and 3D Scaffolds: A Mechanical Study Approach of Stem Cells in Anticancer Therapy
Authors: Metsiou Despoina Nektaria , Kozaniti Foteini K. , Deligianni Despina D.
Published: November 2021

Cell biomechanics plays a major role as a promising biomarker for early cancer diagnosis and prognosis. In the present study, alterations in modulus of elasticity, cell membrane roughness, and migratory potential of MCF-7 (ER+) and SKBR-3 (HER2+) cancer cells were elucidated prior to and post treatment with conditioned medium from human umbilical mesenchymal stem cells (hUMSCs-CM) during static and dynamic cell culture. Moreover, the therapeutic potency of hUMSCs-CM on cancer cell’s viability, migratory potential, and F-actin quantified intensity was addressed in 2D surfaces and 3D scaffolds. Interestingly, alterations in ER+ cancer cells showed a positive effect of treatment upon limiting cell viability, motility, and potential for migration. Moreover, increased post treatment cell stiffness indicated rigid cancer cells with confined cell movement and cytoskeletal alterations with restricted lamellipodia formation, which enhanced these results. On the contrary, the cell viability and the migratory potential were not confined post treatment with hUMSCs-CM on HER2+ cells, possibly due to their intrinsic aggressiveness. The increased post treatment cell viability and the decreased cell stiffness indicated an increased potency for cell movement. Hence, the therapy had no efficacy on HER2+ cells.
Physical Training Increases Erythroferrone Levels in Men
Authors: Dziembowska Inga , Wójcik Małgorzata , Bukowski Jakub , Żekanowska Ewa
Published: November 2021

Intense physical activity contributes to an increased demand for red blood cells, which transport oxygen to working muscles. The purpose of this study was to assess the concentration of erythroferrone (ERFE), the novel marker of erythroid activity in athletes, during the beginning of their training season. The study group consisted of 39 athletes aged 23.24 ± 3.77 years. The study was carried out during the athletes’ preparatory period of the training cycle. The control group consisted of 34 healthy men aged 22.33 ± 2.77 years. The erythropoietic activity was evaluated by determining athletes’ concentrations of erythropoietin (EPO) and erythroferrone (ERFE). The level of physical activity was assessed using the International Physical Activity Questionnaire (IPAQ). In the athletes’ group, we observed higher concentrations of EPO (Me = 12.65 mIU/mL) and ERFE (40.00 pg/mL) compared to the control group (EPO: Me = 5.74 mIU/ml, p = 0.001; ERFE: Me = 25.50 pg/mL, p = 0.0034). The average intensity of physical exercise significantly differentiated the participants as far as EPO and ERFE concentrations. These results suggest that intense physical activity, at least at the beginning of the training season, may stimulate EPO production, which increases ERFE release. This seems to be an adaptative mechanism that provides adequate iron for enhanced erythropoiesis.
Rhamnolipids as a Tool for Eradication of Trichosporon cutaneum Biofilm
Authors: Maťátková Olga , Kolouchová Irena , Lokočová Kristýna , Michailidu Jana , Jaroš Petr , Kulišová Markéta , Řezanka Tomáš , Masák Jan
Published: November 2021

Microbial biofilms formed by pathogenic and antibiotic-resistant microorganisms represent a serious threat for public health in medicine and many industrial branches. Biofilms are involved in many persistent and chronic infections, the biofouling of water and food contamination. Therefore, current research is involved in the development of new treatment strategies. Biofilm is a complex system, and thus all aspects of the measurement and monitoring of its growth and eradication in various conditions, including static and dynamic flow, are issues of great importance. The antibiofilm character of rhamnolipid mixtures produced by four Pseudomonas aeruginosa strains was studied under different conditions. For this purpose, the biofilm of opportunistic pathogen Trichosporon cutaneum was used and treated under static conditions (microscope glass coverslip in a Petri dish) and under dynamic conditions (a single-channel flow cell). The results show that the biological activity of rhamnolipids depends both on their properties and on the conditions of the biofilm formation. Therefore, this aspect must be taken into account when planning the experimental or application design.
Nanoparticles as Vectors to Tackle Cancer
Authors: Duan Chengchen , Townley Helen E
Published: November 2021

The aim of this Special Issue, “Nanoparticles for cancer therapy”, was to offer readers a comprehensive and up-to-date insight into the various applications of nanoparticles in cancer treatments [...]
Piezoelectric Signals in Vascularized Bone Regeneration
Authors: D’Alessandro Delfo , Ricci Claudio , Milazzo Mario , Strangis Giovanna , Forli Francesca , Buda Gabriele , Petrini Mario , Berrettini Stefano , Uddin Mohammed Jasim , Danti Serena , Parchi Paolo
Published: November 2021

The demand for bone substitutes is increasing in Western countries. Bone graft substitutes aim to provide reconstructive surgeons with off-the-shelf alternatives to the natural bone taken from humans or animal species. Under the tissue engineering paradigm, biomaterial scaffolds can be designed by incorporating bone stem cells to decrease the disadvantages of traditional tissue grafts. However, the effective clinical application of tissue-engineered bone is limited by insufficient neovascularization. As bone is a highly vascularized tissue, new strategies to promote both osteogenesis and vasculogenesis within the scaffolds need to be considered for a successful regeneration. It has been demonstrated that bone and blood vases are piezoelectric, namely, electric signals are locally produced upon mechanical stimulation of these tissues. The specific effects of electric charge generation on different cells are not fully understood, but a substantial amount of evidence has suggested their functional and physiological roles. This review summarizes the special contribution of piezoelectricity as a stimulatory signal for bone and vascular tissue regeneration, including osteogenesis, angiogenesis, vascular repair, and tissue engineering, by considering different stem cell sources entailed with osteogenic and angiogenic potential, aimed at collecting the key findings that may enable the development of successful vascularized bone replacements useful in orthopedic and otologic surgery.
Assaying Paenibacillus alvei CsaB-Catalysed Ketalpyruvyltransfer to Saccharides by Measurement of Phosphate Release
Authors: Hager-Mair Fiona F. , Stefanović Cordula , Lim Charlie , Webhofer Katharina , Krauter Simon , Blaukopf Markus , Ludwig Roland , Kosma Paul , Schäffer Christina
Published: November 2021

Ketalpyruvyltransferases belong to a widespread but little investigated class of enzymes, which utilise phosphoenolpyruvate (PEP) for the pyruvylation of saccharides. Pyruvylated saccharides play pivotal biological roles, ranging from protein binding to virulence. Limiting factors for the characterisation of ketalpyruvyltransferases are the availability of cognate acceptor substrates and a straightforward enzyme assay. We report on a fast ketalpyruvyltransferase assay based on the colorimetric detection of phosphate released during pyruvyltransfer from PEP onto the acceptor via complexation with Malachite Green and molybdate. To optimise the assay for the model 4,6-ketalpyruvyl::ManNAc-transferase CsaB from Paenibacillus alvei, a β-d-ManNAc-α-d-GlcNAc-diphosphoryl-11-phenoxyundecyl acceptor mimicking an intermediate of the bacterium’s cell wall glycopolymer biosynthesis pathway, upon which CsaB is naturally active, was produced chemo-enzymatically and used together with recombinant CsaB. Optimal assay conditions were 5 min reaction time at 37 °C and pH 7.5, followed by colour development for 1 h at 37 °C and measurement of absorbance at 620 nm. The structure of the generated pyruvylated product was confirmed by NMR spectroscopy. Using the established assay, the first kinetic constants of a 4,6-ketalpyuvyl::ManNAc-transferase could be determined; upon variation of the acceptor and PEP concentrations, a KM, PEP of 19.50 ± 3.50 µM and kcat, PEP of 0.21 ± 0.01 s−1 as well as a KM, Acceptor of 258 ± 38 µM and a kcat, Acceptor of 0.15 ± 0.01 s−1 were revealed. P. alvei CsaB was inactive on synthetic pNP-β-d-ManNAc and β-d-ManNAc-β-d-GlcNAc-1-OMe, supporting the necessity of a complex acceptor substrate.
Comprehensive Characterization of the Coding and Non-Coding Single Nucleotide Polymorphisms in the Tumor Protein p63 (TP63) Gene Using In Silico Tools
Authors: Akter Shamima , Hossain Shafaat , Ali Md. Ackas , Hosen Md. Ismail , Shekhar Hossain Uddin
Published: November 2021

Single nucleotide polymorphisms (SNPs) help to understand the phenotypic variations in humans. Genome-wide association studies (GWAS) have identified SNPs located in the tumor protein 63 (TP63) locus to be associated with the genetic susceptibility of cancers. However, there is a lack of in-depth characterization of the structural and functional impacts of the SNPs located at the TP63 gene. The current study was designed for the comprehensive characterization of the coding and non-coding SNPs in the human TP63 gene for their functional and structural significance. The functional and structural effects of the SNPs were investigated using a wide variety of computational tools and approaches, including molecular dynamics (MD) simulation. The deleterious impact of eight nonsynonymous SNPs (nsSNPs) affecting protein stability, structure, and functions was measured by using 13 bioinformatics tools. These eight nsSNPs are in highly conserved positions in protein and were predicted to decrease protein stability and have a deleterious impact on the TP63 protein function. Molecular docking analysis showed five nsSNPs to reduce the binding affinity of TP63 protein to DNA with significant results for three SNPs (R319H, G349E, and C347F). Further, MD simulations revealed the possible disruption of TP63 and DNA binding, hampering the essential protein function. PolymiRTS study found five non-coding SNPs in miRNA binding sites, and the GTEx portal recognized five eQTLs SNPs in single tissue of the lung, heart (LV), and cerebral hemisphere (brain). Characterized nsSNPs and non-coding SNPs will help researchers to focus on TP63 gene loci and ascertain their association with certain diseases.
A Review on Development of Bio-Inspired Implants Using 3D Printing
Authors: Raheem Ansheed A. , Hameed Pearlin , Whenish Ruban , Elsen Renold S. , G Aswin , Jaiswal Amit Kumar , Prashanth Konda Gokuldoss , Manivasagam Geetha
Published: November 2021

Biomimetics is an emerging field of science that adapts the working principles from nature to fine-tune the engineering design aspects to mimic biological structure and functions. The application mainly focuses on the development of medical implants for hard and soft tissue replacements. Additive manufacturing or 3D printing is an established processing norm with a superior resolution and control over process parameters than conventional methods and has allowed the incessant amalgamation of biomimetics into material manufacturing, thereby improving the adaptation of biomaterials and implants into the human body. The conventional manufacturing practices had design restrictions that prevented mimicking the natural architecture of human tissues into material manufacturing. However, with additive manufacturing, the material construction happens layer-by-layer over multiple axes simultaneously, thus enabling finer control over material placement, thereby overcoming the design challenge that prevented developing complex human architectures. This review substantiates the dexterity of additive manufacturing in utilizing biomimetics to 3D print ceramic, polymer, and metal implants with excellent resemblance to natural tissue. It also cites some clinical references of experimental and commercial approaches employing biomimetic 3D printing of implants.
Reagentless D-Tagatose Biosensors Based on the Oriented Immobilization of Fructose Dehydrogenase onto Coated Gold Nanoparticles- or Reduced Graphene Oxide-Modified Surfaces: Application in a Prototype Bioreactor
Authors: Šakinytė Ieva , Butkevičius Marius , Gurevičienė Vidutė , Stankevičiūtė Jonita , Meškys Rolandas , Razumienė Julija
Published: November 2021

As electrode nanomaterials, thermally reduced graphene oxide (TRGO) and modified gold nanoparticles (AuNPs) were used to design bioelectrocatalytic systems for reliable D-tagatose monitoring in a long-acting bioreactor where the valuable sweetener D-tagatose was enzymatically produced from a dairy by-product D-galactose. For this goal D-fructose dehydrogenase (FDH) from Gluconobacter industrius immobilized on these electrode nanomaterials by forming three amperometric biosensors: AuNPs coated with 4-mercaptobenzoic acid (AuNP/4-MBA/FDH) or AuNPs coated with 4-aminothiophenol (AuNP/PATP/FDH) monolayer, and a layer of TRGO on graphite (TRGO/FDH) were created. The immobilized FDH due to changes in conformation and spatial orientation onto proposed electrode surfaces catalyzes a direct D-tagatose oxidation reaction. The highest sensitivity for D-tagatose of 0.03 ± 0.002 μA mM−1cm−2 was achieved using TRGO/FDH. The TRGO/FDH was applied in a prototype bioreactor for the quantitative evaluation of bioconversion of D-galactose into D-tagatose by L-arabinose isomerase. The correlation coefficient between two independent analyses of the bioconversion mixture: spectrophotometric and by the biosensor was 0.9974. The investigation of selectivity showed that the biosensor was not active towards D-galactose as a substrate. Operational stability of the biosensor indicated that detection of D-tagatose could be performed during six hours without loss of sensitivity.
Label-free Surface Enhanced Raman Scattering (SERS) on Centrifugal Silver Plasmonic Paper (CSPP): A Novel Methodology for Unprocessed Biofluids Sampling and Analysis
Authors: Esposito Alessandro , Bonifacio Alois , Sergo Valter , Fornasaro Stefano
Published: November 2021

Label-free SERS is a powerful bio-analytical technique in which molecular fingerprinting is combined with localized surface plasmons (LSPs) on metal surfaces to achieve high sensitivity. Silver and gold colloids are among the most common nanostructured substrates used in SERS, but since protein-rich samples such as serum or plasma can hinder the SERS effect due to protein–substrate interactions, they often require a deproteinization step. Moreover, SERS methods based on metal colloids often suffer from a poor reproducibility. Here, we propose a paper-based SERS sampling method in which unprocessed human serum samples are first soaked on paper strips (0.4 × 2 cm2), and then mixed with colloidal silver nanoparticles by centrifugation to obtain a Centrifugal Silver Plasmonic Paper (CSPP). The CSPP methodology has the potential to become a promising tool in bioanalytical SERS applications: it uses common colloidal substrates but without the need for sample deproteinization, while having a good reproducibility both in terms of overall spectral shape (r > 0.96) and absolute intensity (RSD < 10%). Moreover, this methodology allows SERS analysis more than one month after serum collection on the paper strip, facilitating storage and handling of clinical samples (including shipping from clinical sites to labs).
MR Spectroscopy of the Insula: Within- and between-Session Reproducibility of MEGA-PRESS Measurements of GABA+ and Other Metabolites
Authors: Shyu Claire , Elsaid Sonja , Truong Peter , Chavez Sofia , Le Foll Bernard
Published: November 2021

The insula plays a critical role in many neuropsychological disorders. Research investigating its neurochemistry with magnetic resonance spectroscopy (MRS) has been limited compared with cortical regions. Here, we investigate the within-session and between-session reproducibility of metabolite measurements in the insula on a 3T scanner. We measure N-acetylaspartate + N-acetylaspartylglutamate (tNAA), creatine + phosphocreatine (tCr), glycerophosphocholine + phosphocholine (tCho), myo-inositol (Ins), glutamate + glutamine (Glx), and γ-aminobutyric acid (GABA) in one cohort using a j-edited MEGA-PRESS sequence. We measure tNAA, tCr, tCho, Ins, and Glx in another cohort with a standard short-TE PRESS sequence as a reference for the reproducibility metrics. All participants were scanned 4 times identically: 2 back-to-back scans each day, on 2 days. Preprocessing was done using LCModel and Gannet. Reproducibility was determined using Pearson’s r, intraclass-correlation coefficients (ICC), coefficients of variation (CV%), and Bland–Altman plots. A MEGA-PRESS protocol requiring averaged results over two 6:45-min scans yielded reproducible GABA measurements (CV% = 7.15%). This averaging also yielded reproducibility metrics comparable to those from PRESS for the other metabolites. Voxel placement inconsistencies did not affect reproducibility, and no sex differences were found. The data suggest that MEGA-PRESS can reliably measure standard metabolites and GABA in the insula.
Flexible Use of Spatial Frames of Reference for Object–Location Memory in Older Adults
Authors: Ladyka-Wojcik Natalia , Olsen Rosanna K. , Ryan Jennifer D. , Barense Morgan D.
Published: November 2021

In memory, representations of spatial features are stored in different reference frames; features relative to our position are stored egocentrically and features relative to each other are stored allocentrically. Accessing these representations engages many cognitive and neural resources, and so is susceptible to age-related breakdown. Yet, recent findings on the heterogeneity of cognitive function and spatial ability in healthy older adults suggest that aging may not uniformly impact the flexible use of spatial representations. These factors have yet to be explored in a precisely controlled task that explicitly manipulates spatial frames of reference across learning and retrieval. We used a lab-based virtual reality task to investigate the relationship between object–location memory across frames of reference, cognitive status, and self-reported spatial ability. Memory error was measured using Euclidean distance from studied object locations to participants’ responses at testing. Older adults recalled object locations less accurately when they switched between frames of reference from learning to testing, compared with when they remained in the same frame of reference. They also showed an allocentric learning advantage, producing less error when switching from an allocentric to an egocentric frame of reference, compared with the reverse direction of switching. Higher MoCA scores and better self-assessed spatial ability predicted less memory error, especially when learning occurred egocentrically. We suggest that egocentric learning deficits are driven by difficulty in binding multiple viewpoints into a coherent representation. Finally, we highlight the heterogeneity of spatial memory performance in healthy older adults as a potential cognitive marker for neurodegeneration, beyond normal aging.
Epigenetic Small Molecules Rescue Nucleocytoplasmic Transport and DNA Damage Phenotypes in C9ORF72 ALS/FTD
Authors: Ramic Melina , Andrade Nadja S. , Rybin Matthew J. , Esanov Rustam , Wahlestedt Claes , Benatar Michael , Zeier Zane
Published: November 2021

Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disease with available treatments only marginally slowing progression or improving survival. A hexanucleotide repeat expansion mutation in the C9ORF72 gene is the most commonly known genetic cause of both sporadic and familial cases of ALS and frontotemporal dementia (FTD). The C9ORF72 expansion mutation produces five dipeptide repeat proteins (DPRs), and while the mechanistic determinants of DPR-mediated neurotoxicity remain incompletely understood, evidence suggests that disruption of nucleocytoplasmic transport and increased DNA damage contributes to pathology. Therefore, characterizing these disturbances and determining the relative contribution of different DPRs is needed to facilitate the development of novel therapeutics for C9ALS/FTD. To this end, we generated a series of nucleocytoplasmic transport “biosensors”, composed of the green fluorescent protein (GFP), fused to different classes of nuclear localization signals (NLSs) and nuclear export signals (NESs). Using these biosensors in conjunction with automated microscopy, we investigated the role of the three most neurotoxic DPRs (PR, GR, and GA) on seven nuclear import and two export pathways. In addition to other DPRs, we found that PR had pronounced inhibitory effects on the classical nuclear export pathway and several nuclear import pathways. To identify compounds capable of counteracting the effects of PR on nucleocytoplasmic transport, we developed a nucleocytoplasmic transport assay and screened several commercially available compound libraries, totaling 2714 compounds. In addition to restoring nucleocytoplasmic transport efficiencies, hits from the screen also counteract the cytotoxic effects of PR. Selected hits were subsequently tested for their ability to rescue another C9ALS/FTD phenotype—persistent DNA double strand breakage. Overall, we found that DPRs disrupt multiple nucleocytoplasmic transport pathways and we identified small molecules that counteract these effects—resulting in increased viability of PR-expressing cells and decreased DNA damage markers in patient-derived motor neurons. Several HDAC inhibitors were validated as hits, supporting previous studies that show that HDAC inhibitors confer therapeutic effects in neurodegenerative models.
Impact of Early Rhythmic Training on Language Acquisition and Electrophysiological Functioning Underlying Auditory Processing: Feasibility and Preliminary Findings in Typically Developing Infants
Authors: Dondena Chiara , Riva Valentina , Molteni Massimo , Musacchia Gabriella , Cantiani Chiara
Published: November 2021

Previous evidence has shown that early auditory processing impacts later linguistic development, and targeted training implemented at early ages can enhance auditory processing skills, with better expected language development outcomes. This study focuses on typically developing infants and aims to test the feasibility and preliminary efficacy of music training based on active synchronization with complex musical rhythms on the linguistic outcomes and electrophysiological functioning underlying auditory processing. Fifteen infants participated in the training (RTr+) and were compared with two groups of infants not attending any structured activities during the same time frame (RTr−, N = 14). At pre- and post-training, expressive and receptive language skills were assessed using standardized tests, and auditory processing skills were characterized through an electrophysiological non-speech multi-feature paradigm. Results reveal that RTr+ infants showed significantly broader improvement in both expressive and receptive pre-language skills. Moreover, at post-training, they presented an electrophysiological pattern characterized by shorter latency of two peaks (N2* and P2), reflecting a neural change detection process: these shifts in latency go beyond those seen due to maturation alone. These results provide preliminary evidence on the efficacy of our training in improving early linguistic competences, and in modifying the neural underpinnings of auditory processing in infants.
Assessment of Selected Models for FRP-Retrofitted URM Walls under In-Plane Loads
Authors: Hadzima-Nyarko Marijana , Čolak Stanko , Bulajić Borko Đ. , Ademović Naida
Published: November 2021

One way to improve a structure’s total load-bearing capacity during an earthquake is to apply fiber-reinforced polymers (FRP) to unreinforced walls. The study discusses the use of FRP to strengthen unreinforced masonry (URM) structures. Although, many studies were conducted on the FRP strengthening of URM buildings, most of them were experiments to investigate the success of retrofitting approaches, rather than developing a successful design model. A database of 120 FRP-reinforced wall samples was created based on the current literature. Various approaches for calculating the bearing capacity of FRP-reinforced masonry are presented and detailed. The findings of the experiments, which were compiled into a database, were compared to those derived using formulas from the literature and/or building codes, and the model’s limitations are discussed.
Separable Magnetic Fe3O4@MoS2 Composite for Adsorption and Piezo-Catalytic Degradation of Dye
Authors: Zhou Chi , Liu Wencheng , Li Hanqing , Yang Miao , Yang Zixin
Published: November 2021

Well-designed composite catalysts are of increasing concern due to their improved performance compared to individual components. Herein, we designed and synthesized an Fe3O4@MoS2 composite via a simple hydrothermal method. As for the resultant composite, the MoS2 nanolayers presented a novel piezo-catalytic effect, while the Fe3O4 core provided a magnetic separation property. The structure and properties of Fe3O4@MoS2 were determined by relevant experiments. It was found that Fe3O4@MoS2 exhibited enhanced piezo-catalytic degradation of rhodamine B and good magnetic recovery/recycling features. The kobs for rhodamine B degradation over Fe3O4@MoS2 was 0.019 min−1—a little longer than that over MoS2 (0.013 min−1). Moreover, Fe3O4@MoS2 also showed a favorable ability to adsorb rhodamine B in solution, with a saturation adsorption of 26.8 mg/g. Further studies revealed that piezo-electrons, holes, and superoxide anions were key species in the piezo-catalytic degradation of rhodamine B. Notably, the step where oxygen trapped electrons to produce superoxide anions had a significant impact on the degradation of the dye. This work, not limited to the development of a high-performance MoS2-based piezo-catalyst, is expected to provide new insights into the working mechanisms and process profiles of composite piezo-catalysts.
Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents
Authors: Sedova Anastasia , Rucká Lenka , Bojarová Pavla , Glozlová Michaela , Novotný Petr , Křístková Barbora , Pátek Miroslav , Martínková Ludmila
Published: November 2021

Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN−). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from Exidia glandulosa (protein KZV92691.1 designated NitEg by us), which was overproduced in Escherichia coli. The purified NitEg was highly active for fCN with 784 U/mg protein, kcat 927/s and kcat/KM 42/s/mM. It exhibited optimal activities at pH approximately 6–9 and 40–45 °C. It was quite stable in this pH range, and retained approximately 40% activity at 37 °C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30–40%. The removal of 98–100% fCN was achieved for 0.6–100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.