bluuebunny/crossref_metadata_2025 huggingface dataset:
Dysregulated Antibody, Natural Killer Cell and Immune Mediator Profiles in Autoimmune Thyroid Diseases
Authors: Martin Tiphaine C. , Ilieva Kristina M. , Visconti Alessia , Beaumont Michelle , Kiddle Steven J. , Dobson Richard J. B. , Mangino Massimo , Lim Ee Mun , Pezer Marija , Steves Claire J. , Bell Jordana T. , Wilson Scott G. , Lauc Gordan , Roederer Mario , Walsh John P. , Spector Tim D. , Karagiannis Sophia N.
Published: March 2020

The pathogenesis of autoimmune thyroid diseases (AITD) is poorly understood and the association between different immune features and the germline variants involved in AITD are yet unclear. We previously observed systemic depletion of IgG core fucosylation and antennary α1,2 fucosylation in peripheral blood mononuclear cells in AITD, correlated with anti-thyroid peroxidase antibody (TPOAb) levels. Fucose depletion is known to potentiate strong antibody-mediated NK cell activation and enhanced target antigen-expressing cell killing. In autoimmunity, this may translate to autoantibody-mediated immune cell recruitment and attack of self-antigen expressing normal tissues. Hence, we investigated the crosstalk between immune cell traits, secreted proteins, genetic variants and the glycosylation patterns of serum IgG, in a multi-omic and cross-sectional study of 622 individuals from the TwinsUK cohort, 172 of whom were diagnosed with AITD. We observed associations between two genetic variants (rs505922 and rs687621), AITD status, the secretion of Desmoglein-2 protein, and the profile of two IgG N-glycan traits in AITD, but further studies need to be performed to better understand their crosstalk in AITD. On the other side, enhanced afucosylated IgG was positively associated with activatory CD335- CD314+ CD158b+ NK cell subsets. Increased levels of the apoptosis and inflammation markers Caspase-2 and Interleukin-1α positively associated with AITD. Two genetic variants associated with AITD, rs1521 and rs3094228, were also associated with altered expression of the thyrocyte-expressed ligands known to recognize the NK cell immunoreceptors CD314 and CD158b. Our analyses reveal a combination of heightened Fc-active IgG antibodies, effector cells, cytokines and apoptotic signals in AITD, and AITD genetic variants associated with altered expression of thyrocyte-expressed ligands to NK cell immunoreceptors. Together, TPOAb responses, dysregulated immune features, germline variants associated with immunoactivity profiles, are consistent with a positive autoreactive antibody-dependent NK cell-mediated immune response likely drawn to the thyroid gland in AITD.
Yeast as a Model to Understand Actin-Mediated Cellular Functions in Mammals—Illustrated with Four Actin Cytoskeleton Proteins
Authors: Akram Zain , Ahmed Ishtiaq , Mack Heike , Kaur Ramandeep , Silva Richard C. , Castilho Beatriz A. , Friant Sylvie , Sattlegger Evelyn , Munn Alan L.
Published: March 2020

The budding yeast Saccharomyces cerevisiae has an actin cytoskeleton that comprises a set of protein components analogous to those found in the actin cytoskeletons of higher eukaryotes. Furthermore, the actin cytoskeletons of S. cerevisiae and of higher eukaryotes have some similar physiological roles. The genetic tractability of budding yeast and the availability of a stable haploid cell type facilitates the application of molecular genetic approaches to assign functions to the various actin cytoskeleton components. This has provided information that is in general complementary to that provided by studies of the equivalent proteins of higher eukaryotes and hence has enabled a more complete view of the role of these proteins. Several human functional homologues of yeast actin effectors are implicated in diseases. A better understanding of the molecular mechanisms underpinning the functions of these proteins is critical to develop improved therapeutic strategies. In this article we chose as examples four evolutionarily conserved proteins that associate with the actin cytoskeleton: (1) yeast Hof1p/mammalian PSTPIP1, (2) yeast Rvs167p/mammalian BIN1, (3) yeast eEF1A/eEF1A1 and eEF1A2 and (4) yeast Yih1p/mammalian IMPACT. We compare the knowledge on the functions of these actin cytoskeleton-associated proteins that has arisen from studies of their homologues in yeast with information that has been obtained from in vivo studies using live animals or in vitro studies using cultured animal cell lines.
The Intersection of Serine Metabolism and Cellular Dysfunction in Retinal Degeneration
Authors: Sinha Tirthankar , Ikelle Larissa , Naash Muna I. , Al-Ubaidi Muayyad R.
Published: March 2020

In the past, the importance of serine to pathologic or physiologic anomalies was inadequately addressed. Omics research has significantly advanced in the last two decades, and metabolomic data of various tissues has finally brought serine metabolism to the forefront of metabolic research, primarily for its varied role throughout the central nervous system. The retina is one of the most complex neuronal tissues with a multitude of functions. Although recent studies have highlighted the importance of free serine and its derivatives to retinal homeostasis, currently few reviews exist that comprehensively analyze the topic. Here, we address this gap by emphasizing how and why the de novo production and demand for serine is exceptionally elevated in the retina. Many basic physiological functions of the retina require serine. Serine-derived sphingolipids and phosphatidylserine for phagocytosis by the retinal pigment epithelium (RPE) and neuronal crosstalk of the inner retina via D-serine require proper serine metabolism. Moreover, serine is involved in sphingolipid–ceramide balance for both the outer retina and the RPE and the reductive currency generation for the RPE via serine biosynthesis. Finally and perhaps the most vital part of serine metabolism is free radical scavenging in the entire retina via serine-derived scavengers like glycine and GSH. It is hard to imagine that a single tissue could have such a broad and extensive dependency on serine homeostasis. Any dysregulation in serine mechanisms can result in a wide spectrum of retinopathies. Therefore, most critically, this review provides a strong argument for the exploration of serine-based clinical interventions for retinal pathologies.
Exosomes Could Offer New Options to Combat the Long-Term Complications Inflicted by Gestational Diabetes Mellitus
Authors: Floriano Juliana Ferreira , Willis Gareth , Catapano Francesco , de Lima Patrícia Rodrigues , Reis Fabiana Vieira Duarte Souza , Barbosa Angélica Mercia Pascon , Rudge Marilza Vieira Cunha , Emanueli Costanza
Published: March 2020

Gestational diabetes Mellitus (GDM) is a complex clinical condition that promotes pelvic floor myopathy, thus predisposing sufferers to urinary incontinence (UI). GDM usually regresses after birth. Nonetheless, a GDM history is associated with higher risk of subsequently developing type 2 diabetes, cardiovascular diseases (CVD) and UI. Some aspects of the pathophysiology of GDM remain unclear and the associated pathologies (outcomes) are poorly addressed, simultaneously raising public health costs and diminishing women’s quality of life. Exosomes are small extracellular vesicles produced and actively secreted by cells as part of their intercellular communication system. Exosomes are heterogenous in their cargo and depending on the cell sources and environment, they can mediate both pathogenetic and therapeutic functions. With the advancement in knowledge of exosomes, new perspectives have emerged to support the mechanistic understanding, prediction/diagnosis and ultimately, treatment of the post-GMD outcomes. Here, we will review recent advances in knowledge of the role of exosomes in GDM and related areas and discuss the possibilities for translating exosomes as therapeutic agents in the GDM clinical setting.
Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch
Authors: Hilbold Erika , Distl Ottmar , Hoedemaker Martina , Wilkening Sandra , Behr Rüdiger , Rajkovic Aleksandar , Langeheine Marion , Rode Kristina , Jung Klaus , Metzger Julia , Brehm Ralph H. J.
Published: March 2020

Male factor infertility is a problem in today’s society but many underlying causes are still unknown. The generation of a conditional Sertoli cell (SC)-specific connexin 43 (Cx43) knockout mouse line (SCCx43KO) has provided a translational model. Expression of the gap junction protein Cx43 between adjacent SCs as well as between SCs and germ cells (GCs) is known to be essential for the initiation and maintenance of spermatogenesis in different species and men. Adult SCCx43KO males show altered spermatogenesis and are infertile. Thus, the present study aims to identify molecular mechanisms leading to testicular alterations in prepubertal SCCx43KO mice. Transcriptome analysis of 8-, 10- and 12-day-old mice was performed by next-generation sequencing (NGS). Additionally, candidate genes were examined by qRT-PCR and immunohistochemistry. NGS revealed many significantly differentially expressed genes in the SCCx43KO mice. For example, GC-specific genes were mostly downregulated and found to be involved in meiosis and spermatogonial differentiation (e.g., Dmrtb1, Sohlh1). In contrast, SC-specific genes implicated in SC maturation and proliferation were mostly upregulated (e.g., Amh, Fshr). In conclusion, Cx43 in SCs appears to be required for normal progression of the first wave of spermatogenesis, especially for the mitosis-meiosis switch, and also for the regulation of prepubertal SC maturation.
3D Printed Conductive Nanocellulose Scaffolds for the Differentiation of Human Neuroblastoma Cells
Authors: Bordoni Matteo , Karabulut Erdem , Kuzmenko Volodymyr , Fantini Valentina , Pansarasa Orietta , Cereda Cristina , Gatenholm Paul
Published: March 2020

We prepared cellulose nanofibrils-based (CNF), alginate-based and single-walled carbon nanotubes (SWCNT)-based inks for freeform reversible embedding hydrogel (FRESH) 3D bioprinting of conductive scaffolds. The 3D printability of conductive inks was evaluated in terms of their rheological properties. The differentiation of human neuroblastoma cells (SH-SY5Y cell line) was visualized by the confocal microscopy and the scanning electron microscopy techniques. The expression of TUBB3 and Nestin genes was monitored by the RT-qPCR technique. We have demonstrated that the conductive guidelines promote the cell differentiation, regardless of using differentiation factors. It was also shown that the electrical conductivity of the 3D printed scaffolds could be tuned by calcium–induced crosslinking of alginate, and this plays a significant role on neural cell differentiation. Our work provides a protocol for the generation of a realistic in vitro 3D neural model and allows for a better understanding of the pathological mechanisms of neurodegenerative diseases.
Evaluation of Glycosylated PTGS2 in Colorectal Cancer for NSAIDS-Based Adjuvant Therapy
Authors: Venè Roberta , Costa Delfina , Augugliaro Raffaella , Carlone Sebastiano , Scabini Stefano , Casoni Pattacini Gianmaria , Boggio Maurizio , Zupo Simonetta , Grillo Federica , Mastracci Luca , Pitto Francesca , Minghelli Simona , Ferrari Nicoletta , Tosetti Francesca , Romairone Emanuele , Mingari Maria C. , Poggi Alessandro , Benelli Roberto
Published: March 2020

Observational/retrospective studies indicate that prostaglandin-endoperoxide synthase-2 (PTGS2) inhibitors could positively affect colorectal cancer (CRC) patients’ survival after diagnosis. To obtain an acceptable cost/benefit balance, the inclusion of PTGS2 inhibitors in the adjuvant setting needs a selective criterion. We quantified the 72 kDa, CRC-associated, glycosylated form of PTGS2 in 100 frozen CRC specimens and evaluated PTGS2 localization by IHC in the same tumors, scoring tumor epithelial-derived and stroma-derived fractions. We also investigated the involvement of interleukin-1 beta (IL1β) in PTGS2 induction, both in vitro and in CRC lysates. Finally, we used overall survival (OS) as a criterion for patient selection. Glycosylated PTGS2 can be quantified with high sensibility in tissue lysates, but the expression in both tumor and stromal cells limits its use for predictive purposes. Immunohistochemistry (IHC) analysis indicates that stromal PTGS2 expression could exert a protective role on patient OS. Stromal PTGS2 was prevalently expressed by cancer-associated fibroblasts exerting a barrier function near the gut lumen, and it apparently favored the antitumor M1 macrophage population. IL1β was directly linked to gPTGS2 expression both in vitro and in tumors, but its activity was apparently prevalent on the stromal cell population. We suggest that stromal PTGS2 could exert a positive effect on patients OS when expressed in the luminal area of the tumor.
Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification
Authors: Artiach Gonzalo , Carracedo Miguel , Seime Till , Plunde Oscar , Laguna-Fernandez Andres , Matic Ljubica , Franco-Cereceda Anders , Bäck Magnus
Published: March 2020

Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve. Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification. Particularly, proteoglycan 4 (PRG4) has been identified in fibrotic parts of aortic valves. However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined. Here, transcriptomics, histology, and immunohistochemistry were performed in human aortic valves from patients undergoing aortic valve replacement. Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis. PRG4 was significantly upregulated in thickened and calcified regions of aortic valves compared with healthy regions. In addition, mRNA levels of PRG4 positively associated with mRNA for proteins involved in cardiovascular calcification. Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2. In summary, PRG4 was upregulated in the development of AVS and promoted VIC osteogenic differentiation and calcification. These results suggest that an altered valve leaflet proteoglycan composition may play a role in the progression of AVS.
The Role of Tissue-resident γδ T Cells in Stress Surveillance and Tissue Maintenance
Authors: Johnson Margarete D. , Witherden Deborah A. , Havran Wendy L.
Published: March 2020

While forming a minor population in the blood and lymphoid compartments, γδ T cells are significantly enriched within barrier tissues. In addition to providing protection against infection, these tissue-resident γδ T cells play critical roles in tissue homeostasis and repair. γδ T cells in the epidermis and intestinal epithelium produce growth factors and cytokines that are important for the normal turnover and maintenance of surrounding epithelial cells and are additionally required for the efficient recognition of, and response to, tissue damage. A role for tissue-resident γδ T cells is emerging outside of the traditional barrier tissues as well, with recent research indicating that adipose tissue-resident γδ T cells are required for the normal maintenance and function of the adipose tissue compartment. Here we review the functions of tissue-resident γδ T cells in the epidermis, intestinal epithelium, and adipose tissue, and compare the mechanisms of their activation between these sites.
New and Emerging Systemic Therapeutic Options for Advanced Cholangiocarcinoma
Authors: Massironi Sara , Pilla Lorenzo , Elvevi Alessandra , Longarini Raffaella , Rossi Roberta Elisa , Bidoli Paolo , Invernizzi Pietro
Published: March 2020

Cholangiocarcinoma (CCA) represents a disease entity that comprises a heterogeneous group of biliary malignant neoplasms, with variable clinical presentation and severity. It may be classified according to its anatomical location and distinguished in intrahepatic (iCCA), perihilar (pCCA), or distal (dCCA), each subtype implying distinct epidemiology, biology, prognosis, and strategy for clinical management. Its incidence has increased globally over the past few decades, and its mortality rate remains high due to both its biological aggressiveness and resistance to medical therapy. Surgery is the only potentially curative treatment and is the standard approach for resectable CCA; however, more than half of the patients have locally advanced or metastatic disease at presentation. For patients with unresectable CCA, the available systemic therapies are of limited effectiveness. However, the advances of the comprehension of the complex molecular landscape of CCA and its tumor microenvironment could provide new keys to better understand the pathogenesis, the mechanisms of resistance and ultimately to identify promising new therapeutic targets. Recently, clinical trials targeting isocitrate dehydrogenase (IDH)-1 mutations and fibroblast growth factor receptor (FGFR)-2 fusions, as well as immunotherapy showed promising results. All these new and emerging therapeutic options are herein discussed.
Is T Cell Negative Selection a Learning Algorithm?
Authors: Wortel Inge M. N. , Keşmir Can , de Boer Rob J. , Mandl Judith N. , Textor Johannes
Published: March 2020

Our immune system can destroy most cells in our body, an ability that needs to be tightly controlled. To prevent autoimmunity, the thymic medulla exposes developing T cells to normal “self” peptides and prevents any responders from entering the bloodstream. However, a substantial number of self-reactive T cells nevertheless reaches the periphery, implying that T cells do not encounter all self peptides during this negative selection process. It is unclear if T cells can still discriminate foreign peptides from self peptides they haven’t encountered during negative selection. We use an “artificial immune system”—a machine learning model of the T cell repertoire—to investigate how negative selection could alter the recognition of self peptides that are absent from the thymus. Our model reveals a surprising new role for T cell cross-reactivity in this context: moderate T cell cross-reactivity should skew the post-selection repertoire towards peptides that differ systematically from self. Moreover, even some self-like foreign peptides can be distinguished provided that the peptides presented in the thymus are not too similar to each other. Thus, our model predicts that negative selection on a well-chosen subset of self peptides would generate a repertoire that tolerates even “unseen” self peptides better than foreign peptides. This effect would resemble a “generalization” process as it is found in learning systems. We discuss potential experimental approaches to test our theory.
Effect of Lifestyle Intervention in the Concentration of Adipoquines and Branched Chain Amino Acids in Subjects with High Risk of Developing Type 2 Diabetes: Feel4Diabetes Study
Authors: Lamiquiz-Moneo Itziar , Bea Ana M. , Palacios-Pérez Cristian , De Miguel-Etayo Pilar , González-Gil Esther M. , López-Ariño Chuan , Civeira Fernando , Moreno Luis A. , Mateo-Gallego Rocio
Published: March 2020

Introduction: The global prevalence of type 2 diabetes (T2D) is increasing rapidly, especially in low- and middle-income countries and has a high number of associated comorbidities. Plasmatic concentrations of branched chain amino acids (BCAA) and retinol-binding protein 4 (RBP4) have been shown to be elevated in T2D subjects in cross-sectional studies. However, the effect of lifestyle community-based interventions on BCAA and RBP4 concentrations has not yet been analyzed. Material and methods: The Feel4Diabetes study is a school and community-based intervention that identified 360 European families with a high risk of developing T2D according to the FINDRISC questionnaire. Families were randomized in control and intervention groups were followed-up from 2016 to 2018. In the Spanish families, the concentration of BCAA and RBP4 was determined in 266 subjects (115 control and 151 intervention group) that attended the three time-point assessments by colorimetric and ELISA reaction, respectively. Results: Baseline BCAA levels showed positive correlations with the FINDRISC score and glucose impairment (baseline glucose, insulin, and glycated hemoglobin), body mass index, and body weight. The participants receiving the community-based intervention showed a significant decrease in glycated hemoglobin and BCAA levels compared to the control group (p = 0.011 and p < 0.001, respectively). However, baseline RBP4 did not show significant correlations with anthropometric and glycemic parameters, and no significant change was observed in anthropometric parameters and RBP4 concentrations throughout the follow-up. Conclusion: A community-based intervention on lifestyle led to a significant reduction in BCAA levels regardless of weight loss. These findings suggest that this interventional approach could be promising in T2D prevention.
ER Stress Responses: An Emerging Modulator for Innate Immunity
Authors: Di Conza Giusy , Ho Ping-Chih
Published: March 2020

The endoplasmic reticulum (ER) is a critical organelle, storing the majority of calcium and governing protein translation. Thus, it is crucial to keep the homeostasis in all ER components and machineries. The ER stress sensor pathways, including IRE1/sXBP1, PERK/EIf2α and ATF6, orchestrate the major regulatory circuits to ensure ER homeostasis. The embryonic or postnatal lethality that occurs upon genetic depletion of these sensors reveals the essential role of the ER stress pathway in cell biology. In contrast, the impairment or excessive activation of ER stress has been reported to cause or aggravate several diseases such as atherosclerosis, diabetes, NAFDL/NASH, obesity and cancer. Being part of innate immunity, myeloid cells are the first immune cells entering the inflammation site. Upon entry into a metabolically stressed disease environment, activation of ER stress occurs within the myeloid compartment, leading to the modulation of their phenotype and functions. In this review, we discuss causes and consequences of ER stress activation in the myeloid compartment with a special focus on the crosstalk between ER, innate signaling and metabolic environments.
Nuclear Inositides and Inositide-Dependent Signaling Pathways in Myelodysplastic Syndromes
Authors: Xian Jie , Owusu Obeng Eric , Ratti Stefano , Rusciano Isabella , Marvi Maria Vittoria , Fazio Antonietta , De Stefano Alessia , Mongiorgi Sara , Cappellini Alessandra , Ramazzotti Giulia , Manzoli Lucia , Cocco Lucio , Follo Matilde Yung
Published: March 2020

Myelodysplastic syndromes (MDS) are a heterogeneous group of hematological malignancies characterized by peripheral blood cytopenia and abnormal myeloproliferation, as well as a variable risk of evolution into acute myeloid leukemia (AML). The nucleus is a highly organized organelle with several distinct domains where nuclear inositides localize to mediate essential cellular events. Nuclear inositides play a critical role in the modulation of erythropoiesis or myelopoiesis. Here, we briefly review the nuclear structure, the localization of inositides and their metabolic enzymes in subnuclear compartments, and the molecular aspects of nuclear inositides in MDS.
Post-Cardiotomy Parasternal Nerve Block with Bupivacaine May Be Associated with Reduced Post-Operative Opioid Use in Children: A Retrospective Cohort Study
Authors: Moga Francis X. , Lo Galbo Mark D. , Overman David M. , Friedrichsdorf Stefan J.
Published: March 2020

Postoperative pain treatment affects immediate and long-term outcomes in children undergoing cardiac surgery. Opioids, as part of multimodal analgesia, are effective in treating pain, however, they can be disadvantageous due to adverse side effects. Therefore, we assessed whether the local anesthetic bupivacaine as a parasternal nerve block in children post-cardiac surgery is an effective adjunct to pain management. This was a retrospective cohort study of all patients who underwent cardiothoracic surgery via median sternotomy at a large children’s hospital between November 2011 and February 2014 with and without bupivacaine following the introduction of perioperative bupivacaine in late 2012 on a single unit. 62 out of 148 patients (age 3–17 years) who received bupivacaine demonstrated decreased postoperative opioid use. Within one day of surgery, patients who received bupivacaine required, on average, 0.57 mg/kg (95% CI, 0.46 to 0.68) of total morphine equivalent compared to 0.93 mg/kg (95% CI, 0.80 to 1.06) for patients who did not receive bupivacaine. This difference was statistically significant after adjusting for potential confounders (p-value = 0.002). Length of stay and intubation were shorter on average among patients who received bupivacaine, but these differences were not statistically significant after adjusting for potential confounders. The study results seem to suggest that the perioperative administration of bupivacaine may reduce opioid usage among children post-cardiotomy.
Micro and Macro-Tribology Behavior of a Hierarchical Architecture of a Multilayer TaN/Ta Hard Coating
Authors: Rivera-Tello César D. , Broitman E. , Flores-Ruiz Francisco Javier , Perez-Alvarez J. , Flores-Jiménez M. , Jiménez O. , Flores M.
Published: March 2020

The micro- and macro-tribological behaviors of a novel hierarchical TaN/Ta coating deposited on Ti6Al4V biomedical alloy by direct current magnetron sputtering were analyzed in the present work. This analysis was associated with the morphological, structural, and mechanical properties, as well as the roughness changes during and after the tribological tests. The wear track of the coating after the macro-tribology tests was evaluated by Raman spectroscopy in order to detect the compounds formed as a result of the tribo-reactions that occurred during the tests. Micro- and macro-tribology behaviors showed a significant wear rate reduction of the hierarchical coating in comparison to the Ti6Al4V substrate. For the case of the micro-tribology tests, this reduction was attributed to the high hardness of the coating (31.4 GPa); however, this hardness caused a considerable increment in the friction coefficient. On the other hand, the macro-tribology performance was associated with the hardness and the ability of the hierarchical architecture to prevent the propagation of cracks. Moreover, the friction coefficient increased considerably at the end of the test; this increment was associated with the tantalum oxides in the wear track detected by Raman spectroscopy.
Fabrications of Hetero-Junction Schottky Diodes by Electrodeposition of Nano-Structured CuInSe2 Materials Using Different Upper Electrodes
Authors: Liu Jing , Liu Kuo-Wei , Houng Mau-Phon , Yang Cheng-Fu
Published: March 2020

In this study, CuInSe2 (CIS) films (CIS-TFs) and nanorods (CIS-NRs) were successfully deposited on Mo/glass and p+-silicon (p+-Si) using an electrodeposition method. Anodic aluminum oxide (AAO) was used as the template when the CIS-NRs were deposited. Pt, indium tin oxide (ITO), and Ag were deposited as the upper electrodes using a sputtering method to form the hetero-junction devices of Pt/CIS-NRs/p+-Si, ITO/CIS-NRs/p+-Si, ITO/CIS-TF/Mo/glass, and Ag/CIS-NRs/p+-Si, respectively. When p+-Si was used as the substrate, Al was deposited on p+-Si to form negative electrodes for the devices of Pt/CIS-NRs/p+-Si, ITO/CIS-NRs/p+-Si, and Ag/CIS-NRs/p+-Si. The current–voltage properties of all the hetero-junction devices were measured and we found that the hetero-junction of ITO/CIS-NRs/p+-Si, ITO/CIS-TF/Mo/glass, and Ag/CIS-NRs/p+-Si devices revealed the properties of Schottky diodes but the hetero-junction device of Pt/CIS-NRs/p+-Si device did not. The reason for the cause of the differences between these hetero-junction devices was investigated for this study.
Sacrificial Thermally Sprayed Aluminium Coatings for Marine Environments: A Review
Authors: Syrek-Gerstenkorn Berenika , Paul Shiladitya , Davenport Alison J
Published: March 2020

One of the corrosion mitigation methods that is used for the protection of steel operating in seawater environments involves the application of sacrificial metallic coatings (such as aluminium, zinc, and their alloys). This paper reviews current knowledge about thermally-sprayed (TS) and cold-sprayed (CS) Al coatings for the corrosion protection of steel. It also summarises the key findings of the substantial amount of work that has been devoted to understanding mechanisms and the parameters that control the performance of TS Al coatings, such as the spraying method and its parameters like coating thickness and the application of sealer. The paper includes suggestions for areas of further research that could lead to the development of more resilient and longer-lasting coatings, based on the results from both laboratory and field tests that have been published in the literature. It also highlights the need for conducting simulated laboratory tests at conditions of intended service and the importance of long-term testing.
Near Atomic Row Matching in the Interface Analyzed in Both Direct and Reciprocal Space
Authors: Gu Xinfu
Published: March 2020

Reproducible crystallographic features between new phase and matrix are often observed during phase transformation, including orientation relationship, interfacial orientation, morphology, and so on. The geometrical matching in the interface is the key to understanding the preferred transformation crystallography. Recently, a new geometrical method emphasizing the atomic row matching in the interface, the so-called near row matching method, has been proposed to predict the preferred orientations between two arbitrary crystals. In this work, this method originally expressed in direct space was further extended to the reciprocal space. These two methods were implemented in our free software PTClab (version 1.19). It is found that these two expressions are nearly equivalent. As the near row matching in reciprocal space could be directly measured by the diffraction patterns with transmission electron microscopy (TEM), the condition of atomic row matching would be easily identified in reciprocal space during TEM work, and could be applied to rationalize the experimental observations. Several examples in bothsmall and large misfit alloy systems are shown to apply the near tow matching method in both direct and reciprocal space. Furthermore, the row matching method is compared with other models, and there are some crucial aspects that need extra attention when being applied to prediction.
Bicontinuous Cubic and Hexagonal Columnar Liquid Crystalline Ion-Conductors at Room Temperature in Ion-Doped Dendritic Amphiphiles
Authors: Do Thi Huyen , Kim Ho-Joong , Nguyen Manh Linh , Cho Byoung-Ki
Published: March 2020

A bicontinuous cubic (Cubbi) liquid crystalline (LC) phase consisting of three dimensional (3D) conducting networks is a promising structural platform for ion-conductors. For practical applications using this fascinating LC structure, it is necessary to suppress crystallization at room temperature (RT). Herein, we report the Cubbi structure at RT and the morphology–dependent conduction behavior in ionic samples of a non-crystallizable dendritic amphiphile. In the molecular design, branched alkyl chains were used as an ionophobic part instead of crystallizable linear alkyl chains. Two ionic samples with Cubbi and hexagonal columnar (Colhex) LC phases at RT were prepared by adding different amounts of lithium salt to the amphiphile. Impedance analysis demonstrated that the Cubbi phase contributed to the faster ion-conduction to a larger extent than the Colhex phase due to the 3D ionic networks of the Cubbi phase. In addition, the temperature–dependent impedance and electric modulus data provided information regarding the phase transition from microphase-separated phase to molecularly mixed liquid phase.
Data on Creative Industries Ventures’ Performance Influenced by Four Networking Types: Designing Strategies for a Sample of Female Entrepreneurs with the Use of Multiple Criteria Analysis
Authors: Mylonas Naoum , Manolitzas Panagiotis , Grigoroudis E.
Published: March 2020

This paper presents data that investigates the creative industries ventures’ performance affected by four different types of networking, namely the social, the professional, the family, and that with public sector organizations. Three hundred and seventy-one questionnaires have been collected for the assessment of networking impact on venture performance. In order to examine the ventures’ performance levels of the female entrepreneurs or self-employed in the creative industries of Greece, we use a multiple criteria method. Based on the data analysis, the most important criterion for the female entrepreneurs in the creative industries to perform highly is professional networking while the least important is observed in the criterion of family networking.
A Novel, Circulating Tumor Cell Enrichment Method Reduces ARv7 False Positivity in Patients with Castration-Resistant Prostate Cancer
Authors: Nakasato Takehiko , Kusaka Chiho , Ota Mika , Hasebe Yuki , Ueda Kumiko , Unoki Tsutomu , Oshinomi Kazuhiko , Morita Jun , Maeda Yoshiko , Shichijo Takeshi , Naoe Michio , Ogawa Yoshio
Published: March 2020

Background: The AR-V7 splice variant is a cause of castration-resistant prostate cancer (CRPC). However, testing for the presence of AR-V7 by real-time polymerase chain reaction (RT-PCR) shows AR-V7 positivity in healthy individuals. We hypothesized that the positivity reflects contamination by hematopoietic cells. We tried a novel circulating tumor cell (CTC) enrichment instrument, using Celsee, to clear hematopoietic cells. Methods: We tested whole blood or Celsee-enriched samples for AR-V7 by RT-PCR, and included samples from 41 CRPC patients undergoing sequential therapy. We evaluated the associations between AR-V7 status and clinical factors. We evaluated factors affecting AR-V7 positivity. Results: AR-V7 positivity was lower in Celsee-enriched than in whole blood specimens. AR-V7 and clinical factors did not predict the therapy effectiveness. We found no significant differences in the effectiveness of enzalutamide/abiraterone (Enz/Abi) upon AR-V7 evaluation. All AR-V7 positive patients had resistance to Enz/Abi. Docetaxel (DTX), cabazitaxel (CBZ), and Radium223 treatment showed no significant difference in the treatment effectiveness, regardless of AR-V7 presence. AR-V7 was more frequently positive than Extent of disease (EOD) 2 in cases with bone metastases. Conclusion: Celsee CTC enrichment suppresses AR-V7 false positivity. All AR-V7 positive patients presented resistance to Enz/Abi. DTX, CBZ, and Radium223 were effective and remain treatment options. AR-V7 positivity should progressively appear in patients with advanced bone metastases.
Averaging Is Probably Not the Optimum Way of Aggregating Parameters in Federated Learning
Authors: Xiao Peng , Cheng Samuel , Stankovic Vladimir , Vukobratovic Dejan
Published: March 2020

Federated learning is a decentralized topology of deep learning, that trains a shared model through data distributed among each client (like mobile phones, wearable devices), in order to ensure data privacy by avoiding raw data exposed in data center (server). After each client computes a new model parameter by stochastic gradient descent (SGD) based on their own local data, these locally-computed parameters will be aggregated to generate an updated global model. Many current state-of-the-art studies aggregate different client-computed parameters by averaging them, but none theoretically explains why averaging parameters is a good approach. In this paper, we treat each client computed parameter as a random vector because of the stochastic properties of SGD, and estimate mutual information between two client computed parameters at different training phases using two methods in two learning tasks. The results confirm the correlation between different clients and show an increasing trend of mutual information with training iteration. However, when we further compute the distance between client computed parameters, we find that parameters are getting more correlated while not getting closer. This phenomenon suggests that averaging parameters may not be the optimum way of aggregating trained parameters.
Conditional Rényi Divergences and Horse Betting
Authors: Bleuler Cédric , Lapidoth Amos , Pfister Christoph
Published: March 2020

Motivated by a horse betting problem, a new conditional Rényi divergence is introduced. It is compared with the conditional Rényi divergences that appear in the definitions of the dependence measures by Csiszár and Sibson, and the properties of all three are studied with emphasis on their behavior under data processing. In the same way that Csiszár’s and Sibson’s conditional divergence lead to the respective dependence measures, so does the new conditional divergence lead to the Lapidoth–Pfister mutual information. Moreover, the new conditional divergence is also related to the Arimoto–Rényi conditional entropy and to Arimoto’s measure of dependence. In the second part of the paper, the horse betting problem is analyzed where, instead of Kelly’s expected log-wealth criterion, a more general family of power-mean utility functions is considered. The key role in the analysis is played by the Rényi divergence, and in the setting where the gambler has access to side information, the new conditional Rényi divergence is key. The setting with side information also provides another operational meaning to the Lapidoth–Pfister mutual information. Finally, a universal strategy for independent and identically distributed races is presented that—without knowing the winning probabilities or the parameter of the utility function—asymptotically maximizes the gambler’s utility function.
Entropy in Heart Rate Dynamics Reflects How HRV-Biofeedback Training Improves Neurovisceral Complexity during Stress-Cognition Interactions
Authors: Deschodt-Arsac Veronique , Blons Estelle , Gilfriche Pierre , Spiluttini Beatrice , Arsac Laurent M.
Published: March 2020

Despite considerable appeal, the growing appreciation of biosignals complexity reflects that system complexity needs additional support. A dynamically coordinated network of neurovisceral integration has been described that links prefrontal-subcortical inhibitory circuits to vagally-mediated heart rate variability. Chronic stress is known to alter network interactions by impairing amygdala functional connectivity. HRV-biofeedback training can counteract stress defects. We hypothesized the great value of an entropy-based approach of beat-to-beat biosignals to illustrate how HRVB training restores neurovisceral complexity, which should be reflected in signal complexity. In thirteen moderately-stressed participants, we obtained vagal tone markers and psychological indexes (state anxiety, cognitive workload, and Perceived Stress Scale) before and after five-weeks of daily HRVB training, at rest and during stressful cognitive tasking. Refined Composite Multiscale Entropy (RCMSE) was computed over short time scales as a marker of signal complexity. Heightened vagal tone at rest and during stressful tasking illustrates training benefits in the brain-to-heart circuitry. The entropy index reached the highest significance levels in both variance and ROC curves analyses. Restored vagal activity at rest correlated with gain in entropy. We conclude that HRVB training is efficient in restoring healthy neurovisceral complexity and stress defense, which is reflected in HRV signal complexity. The very mechanisms that are involved in system complexity remain to be elucidated, despite abundant literature existing on the role played by amygdala in brain interconnections.
Augmentation of Dispersion Entropy for Handling Missing and Outlier Samples in Physiological Signal Monitoring
Authors: Kafantaris Evangelos , Piper Ian , Lo Tsz-Yan Milly , Escudero Javier
Published: March 2020

Entropy quantification algorithms are becoming a prominent tool for the physiological monitoring of individuals through the effective measurement of irregularity in biological signals. However, to ensure their effective adaptation in monitoring applications, the performance of these algorithms needs to be robust when analysing time-series containing missing and outlier samples, which are common occurrence in physiological monitoring setups such as wearable devices and intensive care units. This paper focuses on augmenting Dispersion Entropy (DisEn) by introducing novel variations of the algorithm for improved performance in such applications. The original algorithm and its variations are tested under different experimental setups that are replicated across heart rate interval, electroencephalogram, and respiratory impedance time-series. Our results indicate that the algorithmic variations of DisEn achieve considerable improvements in performance while our analysis signifies that, in consensus with previous research, outlier samples can have a major impact in the performance of entropy quantification algorithms. Consequently, the presented variations can aid the implementation of DisEn to physiological monitoring applications through the mitigation of the disruptive effect of missing and outlier samples.
Evaluation of Harmonic Contributions for Multi Harmonic Sources System Based on Mixed Entropy Screening and an Improved Independent Component Analysis Method
Authors: Zhao Jinshuai , Yang Honggeng , Ma Xiaoyang , Xu Fangwei
Published: March 2020

Evaluating the harmonic contributions of each nonlinear customer is important for harmonic mitigation in a power system with diverse and complex harmonic sources. The existing evaluation methods have two shortcomings: (1) the calculation accuracy is easily affected by background harmonics fluctuation; and (2) they rely on Global Positioning System (GPS) measurements, which is not economic when widely applied. In this paper, based on the properties of asynchronous measurements, we propose a model for evaluating harmonic contributions without GPS technology. In addition, based on the Gaussianity of the measured harmonic data, a mixed entropy screening mechanism is proposed to assess the fluctuation degree of the background harmonics for each data segment. Only the segments with relatively stable background harmonics are chosen for calculation, which reduces the impacts of the background harmonics in a certain degree. Additionally, complex independent component analysis, as a potential method to this field, is improved in this paper. During the calculation process, the sparseness of the mixed matrix in this method is used to reduce the optimization dimension and enhance the evaluation accuracy. The validity and the effectiveness of the proposed methods are verified through simulations and field case studies.
An Efficient Alert Aggregation Method Based on Conditional Rough Entropy and Knowledge Granularity
Authors: Sun Jiaxuan , Gu Lize , Chen Kaiyuan
Published: March 2020

With the emergence of network security issues, various security devices that generate a large number of logs and alerts are widely used. This paper proposes an alert aggregation scheme that is based on conditional rough entropy and knowledge granularity to solve the problem of repetitive and redundant alert information in network security devices. Firstly, we use conditional rough entropy and knowledge granularity to determine the attribute weights. This method can determine the different important attributes and their weights for different types of attacks. We can calculate the similarity value of two alerts by weighting based on the results of attribute weighting. Subsequently, the sliding time window method is used to aggregate the alerts whose similarity value is larger than a threshold, which is set to reduce the redundant alerts. Finally, the proposed scheme is applied to the CIC-IDS 2018 dataset and the DARPA 98 dataset. The experimental results show that this method can effectively reduce the redundant alerts and improve the efficiency of data processing, thus providing accurate and concise data for the next stage of alert fusion and analysis.
Effects of Diffusion Coefficients and Permanent Charge on Reversal Potentials in Ionic Channels
Authors: Mofidi Hamid , Eisenberg Bob , Liu Weishi
Published: March 2020

In this work, the dependence of reversal potentials and zero-current fluxes on diffusion coefficients are examined for ionic flows through membrane channels. The study is conducted for the setup of a simple structure defined by the profile of permanent charges with two mobile ion species, one positively charged (cation) and one negatively charged (anion). Numerical observations are obtained from analytical results established using geometric singular perturbation analysis of classical Poisson–Nernst–Planck models. For 1:1 ionic mixtures with arbitrary diffusion constants, Mofidi and Liu (arXiv:1909.01192) conducted a rigorous mathematical analysis and derived an equation for reversal potentials. We summarize and extend these results with numerical observations for biological relevant situations. The numerical investigations on profiles of the electrochemical potentials, ion concentrations, and electrical potential across ion channels are also presented for the zero-current case. Moreover, the dependence of current and fluxes on voltages and permanent charges is investigated. In the opinion of the authors, many results in the paper are not intuitive, and it is difficult, if not impossible, to reveal all cases without investigations of this type.
Parents’, Teachers’ and Principals’ Views on Parental Involvement in Secondary Education Schools in Greece
Authors: Anastasiou Sophia , Papagianni Aggeliki
Published: March 2020

The objective of the present study was to investigate parents’, teachers’ and principals’ views on parental involvement (PI) in Secondary Education Schools in Greece. The research was based on a survey among parents (n = 54), teachers (n = 84) and principals (n = 12) in twelve Secondary Education Schools in Magnesia Region in central East Greece. Different views between each group were exhibited on PI in educational issues, decision making or creating links and communication between the school and the local community. Teachers expressed the view that workload and parental attitudes are factors which discourage parental involvement in their school units. Parents felt that teachers’ professionalism, lack of teachers’ training on parental involvement and parents who hesitate talking to teachers were significant barriers for PI in their school units. School principals agreed with parents and teachers on the barriers established due to teachers’ professionalism and parents’ hesitation in talking to teachers as significant factors which discourage PI in their school units. Contrarily to teachers’ views, school principals expressed their willingness to increase PI in teachers’ and school evaluation. School leaders should explore the possibility of organising meetings with teachers and parents to reduce barriers and misconceptions, paving the way for communication between the school unit and parents, increasing the positive outcomes of PI in school management and students’ success.
Uncertainty Quantification Spectral Technique for the Stochastic Point Reactor with Random Parameters
Authors: Alaskary Safa , El-Beltagy Mohamed
Published: March 2020

The stochastic point reactor with random parameters is considered in this work. The hybrid uncertain variations—noise and random parameters—are analyzed with the spectral techniques for the efficiency and high rates of convergence. The proposed hybrid technique enables one to derive an equivalent deterministic system that can be solved to get the mean solution and deviations due to each uncertainty. The contributions of different sources uncertainties can be decomposed and quantified. The deviations in the thermal hydraulics are also computed in the current work. Two model reactors are tested with the proposed technique and the comparisons show the advantages and efficiency compared with the other techniques.