bluuebunny/crossref_metadata_2025 huggingface dataset:
English Writing Performance of Students in Kurir Langit Barru Modern Mosque
Authors: Syarifuddin Ervina , Dollah Syarifuddin , Aeni Nur
Published: March 2023

This study aimed to determine students’ English writing performance in Kurir Langit Barru Modern Mosque. This study used a descriptive quantitative research design with a survey method. The purposive sampling technique was used to answer the research question with a total sample was 32 students. The data of the study was obtained using a writing test. The data analysis showed that the total mean score of students’ performances in writing the descriptive text was 69.68 and was categorized as good. This means that students in Kurir Langit Barru had good English writing performance, they do not have any difficulties in writing a descriptive text, although in some aspects their performance was fair.
Junior High School Teachers' Perception of the Use of Digital Mind Maps
Authors: Agustina Imas Wahyu , Zulfi Nada Amalia
Published: March 2023

In support of developing 21st-century skills, this research aims to investigate Junior High School teachers' perceptions of the use of digital mind maps in learning English subjects. A questionnaire adapted from the Sabbah (2015) and Faste & Lin (2012) models was used to find out the teachers' perception of (a) the use of digital mind map software, and (b) the use of digital mind maps in English learning. Interviews were also done afterward to confirm the questionnaire results. It was found that most respondents believed that digital mind maps were more practical to do, enable work to finish faster and give room for creativity. Mind maps can be used for any topics, genres, skills, and language focus, though mind maps for listening need more exploration. It is suggested that further research involving more respondents in more specific limitations is conducted.
Students' Perception of English Learning toward Their Current Professions
Authors: Irawan Bambang , Arisandi Bobi
Published: March 2023

Abstract
Globalization era demands employees who have a proficient English skill so that international business activity can run well. University as a place which provides a skillful employee is forced to create a practical English learning. However, it is not an easy task to bridge the demand and reality of teaching and learning in the classroom. Based on pre-observation, it was revealed that some of students were not able to implement the knowledge that they have learnt into their current work. Related to the issue, researchers conducted research to find out students' perception on what they learn related to English study and how it affects their current job or profession. The participant of this research were the seventh semester students of English Literature of Pamulang University. They were chosen as participants using purposive sampling. This research itself was conducted at English Letters Department of Pamulang University from December 2020 to January 2021. In conducting this research, qualitative research was chosen as research design. There was one type of data collecting techniques which is questionnaire. The data were analyzed simultaneously using Creswell's (2007) theory called data analysis spiral in order to get a strong and comprehensive conclusion toward the issue.
Key words: Current Profession, English Learning, Students' Perception
Studentsâ€™ Perception on the Use of Google Classroom in Learning English: A Case Study at A State Islamic Secondary School
Authors: Widiyastuti Nanik , Syarifudin Syarifudin , Ariawan Soni
Published: March 2023

The purpose of this study was to determine students' perceptions of online English learning during the covid-19 pandemic at State Islamic Secondary School (MTsN) 3 Mataram. This research is a descriptive research with a qualitative approach. Data was collected through a questionnaire which involved 40 students from class VIII-A. Based on the data analysis from questionnaire, it can be concluded that the students neutrally perceive the utilization of Google Classroom as they found several challenges such as absence of interaction room, lack of internet access etc. However, the interview results indicated that most of them have a positive perception on the use of Google Classroom during the COVID-19 pandemic due to ease of use, the flexibility and effectiveness as well as its access. It is suggested that teachers should be able to adjust their teaching strategy by considering studentsâ€™ factual condition as well as preferences.
Teachers' Code-Switching in English Language Setting
Authors: Siregar Sabar Hernika
Published: March 2023

This research aimed to determine the most commonly utilized forms and the reasons for using the code-switching strategy in the context of language teaching and learning. The subjects were three teachers who practiced their teaching at different English language courses in Pekanbaru. A qualitative design was employed as the method of the research. Moreover, the data collection was conducted through observation of video and audio recordings of the classes and interviews with the teachers. Based on data analysis, the teachers implemented intra-sentential and inter-sentential code-switching. Furthermore, the results revealed that teachers consciously employed this method for multiple reasons, such as achieving communication goals, managing classrooms, and as a discourse method.
Buku Ilustrasi sebagai Media Pengenalan Tradisi Budaya Gresik bagi Anak Usia Sekolah Dasar
Authors: Rohmah Nur Adilah Luthfiyyatur , Dwitasari Putri , Wahyurini Octaviyanti Dwi , Sayatman Sayatman
Published: March 2023

AbstrakGresik merupakan salah satu kabupaten yang mempunyai banyak kebudayaan lokal yang dilakukan secara turun temurun.  Kebudayaan lokal seperti Rebo Wekasan, Pencak Macan, dan Tradisi Kolak Ayam mulai jarang diketahui oleh anak-anak. Oleh karena itu diperlukan adanya upaya pengenalan tradisi budaya kepada anak sejak dini, untuk mencegah hilangnya potensi pengetahuan anak-anak terhadap nilai tradisi kebudayaan lokal. Perancangan ini menggunakan beberapa metode seperti; depth interview, studi eksperimental, studi literatur terhadap jurnal, internet, dan post-test. Hasil dari perancangan ini berupa buku ilustrasi dengan konsep yang dikuatkan pada gambar dan konten dengan tujuan untuk memudahkan audiens mengikuti perjalanan konten yang terdapat di dalam buku. Melalui buku ilustrasi ini diharapkan menjadi media pengenalan terkait tradisi budaya khas Gresik bagi masyarakat khususnya oleh anak-anak. Kata Kunci: Anak, Budaya, Buku Ilustrasi, Gresik, Media Pengenalan, Tradisi  AbstractGresik is a district that has a lot of passed-down local cultures from generation to generation. Local cultures or traditions such as Wednesday Wekasan, Pencak Macan, and the Kolak Ayam are rarely known by children. Therefore, it is necessary to introduce cultural traditions to children at an early age, to prevent the impact lost on the potential of children's knowledge of the value of local cultural traditions. In this design, several methods are used, such as depth interviews, experimental studies, literature studies in journals, the internet, and posttests. The result of this design is in the form of an illustrated book with concepts that are reinforced in images and content with the aim of making it easier for the audience to follow the journey of the content contained in the book. Through this illustration book, it is hoped that it will become an introduction to Gresik's cultural traditions for the community, especially children. Keyword: Children, Cultural, Gresik, Illustration Book, Introductory Media, Tradition
Perancangan Motion Graphic “Awas Predator Online” sebagai Pencegahan Kasus Cyber Child Grooming
Authors: Yuwono Inez Fiona , Saputra M. Garry
Published: March 2023

AbstrakMeningkatnya penggunaan teknologi internet dalam kehidupan sehari-hari menyebabkan kenaikan drastis dalam kasus KBGO, salah satunya adalah cyber child grooming. Cyber child grooming mempermainkan psikologis anak dengan penggunaan teknologi agar korban mau menuruti keinginan pelaku yang berujung ke pelecehan seksual terhadap anak. Orangtua memiliki peranan penting untuk melindungi anak mereka sehingga mendorong pembuatan perancangan motion graphic mengenai bahaya cyber child grooming dan pencegahannya agar dapat menjadi media edukasi yang membantu orangtua untuk mengedukasi anaknya. Dengan metode kualitatif pendekatan deskriptif, perancangan pipeline animation, video animasi motion graphic dibuat dengan gaya visual flat design dan narasi yang telah disederhanakan agar dapat dimengerti oleh target audiens dengan pembahasan mengenai deskripsi KBGO dan cyber child grooming, 6 cara umum yang dilakukan pelaku, dampak yang dihasilkan, pencegahan yang dilihat dari 4 sisi yaitu dari segi privasi tubuh dan data diri, segi game online, segi media sosial, dan segi psikologis anak. Diharapkan media karya utama dapat membantu menambah wawasan dan kesadaran orangtua mengenai cyber child grooming, menjadi media edukasi untuk membantu orangtua mengedukasi anaknya, serta mengurangi kasus KBGO dan cyber child grooming di masa depan. Kata Kunci: cyber child grooming, KBGO, motion graphic AbstractThe increase of online technology users in everyday lifes, also increase the online gender-based violence which one of them is cyber child grooming. Cyber child grooming manipulate a child’s psychology with technology so the child will want to do everything the perpetrator says. Parents have important role to ensure their children’s safety which drives the plan to design a motion graphic about the danger of cyber child grooming and how to prevent it so it can be an educative media to help parents educate their children. With qualitative method and descriptive approach, pipeline animation planning, the motion graphic animated video was made with flat design visual style and simplified narration so it can be easily understood by the audience which discuss about online gender-based and cyber child grooming description, 6 usual method of the perpetrator, the impact they make, how to prevent it from 4 sides, body and personal information privacy, online games, social media, and the child psychology. Hopefully the video can help increase parents’ awareness about cyber child grooming, becomes an educative media to help parents educate their children, decrease the online gender-based violence and cyber child grooming cases in the future. Keyword: cyber child grooming, online gender-based violence, motion graphic
Akulturasi Budaya Warak Ngendhog dalam Bentuk Film Animasi 3D Si Warik
Authors: Nathiania Nita Virena , Kadiasti Ristia , Hastuti Khafiizh , Prayoga Adita Surya
Published: March 2023

AbstrakPelestarian wujud akulturasi budaya dalam bentuk Warak Ngendhog memiliki ancaman pergeseran budaya dengan gencarnya IP karakter dari luar negeri di era globalisasi. Culture knowledge menjadi solusi agar dapat menanamkan warisan budaya Warak Ngendhog kepada generasi selanjutnya dalam bentuk pesan pendidikan. Intelectual Property dalam bentuk karakter Warak Ngendhog yang kemudian diimplementasikan dalam bentuk film animasi 3D dengan balutan cerita yang sarat akan pesan pendidikan untuk menerapkan culture knowledge kepada generasi selanjutnya sesuai dengan target audience. Agar pesan pendidikan tersebut tersampaikan dengan baik kepada target audience, kemudian diramu dalam bentuk visual storytelling model triangulasi Alvanov Zpalanzani yang membentuk tiga struktur aspek pada visualisasi dan alur penceritaan dan juga disempurnakan dengan teori Bahasa Rupa dari aspek visualisasi untuk mendapatkan luaran film animasi 3D yang tepat kepada sasaran audience. Hasilnya adalah kesesuaian pesan cultural knowledge yang disisipkan ke berbagai adegan di film animasi Si Warik. Kata Kunci: Akulturasi, Bahasa Rupa, Visual storytelling, Warak Ngendhog AbstractThe preservation of the cultural acculturation form of Warak Ngendhog has the threat of cultural shifts with the incessant IP characters from abroad in the era of globalization. Cultural knowledge becomes a solution to instill the cultural heritage of Warak Ngendhog to the next generation through educational messages. Intellectual Property in the form of Warak Ngendhog character is then implemented through a 3D animated film with a story that is full of educational messages to apply cultural knowledge to the next generation according to the target audience. For the educational message to be conveyed well to the target audience, it is then mixed through visual storytelling by Alvanov Zpalanzani's triangulation model which forms three aspect structures in visualization and storytelling flow and is also refined with the theory of Visual Language from the visualization aspect to get the right 3D animated film output to the target audience. the result is the appropriateness of the message of cultural knowledge that is inserted into various scenes in the animated film Si Warik. Keyword: Acculturation, Visual Language, Visual storytelling, Warak Ngendhog
Derivative Images
Authors: Watt Calum
Published: March 2023

A theoretical, interdisciplinary reading of French film and literary texts inspired by the 2008 global financial crisis
Draws on ideas by Peter Szendy, Yves Citton, Jonathan Beller and Christophe Hanna to theorise the derivative as a metaphor for thinking about the nature of contemporary audio-visual images
Cuts across medial boundaries to analyse works by Sophie Bruneau, Paul Grivas, Jérôme Kerviel, Mathieu Larnaudie, Frédéric Lordon, Anna Malagrida, Société Réaliste and others
Shows how such French-language writers, artists and filmmakers engage with the financial mechanisms at the heart of the 2008 crash in order to put them into images
Focused on French cultural responses to the 2008 global financial crisis in cinema, literature and theory, Derivative Images offers detailed analyses of post-2008 French-language works, including Les Effondrés (2010), Le Grand Retournement (2013) and L'Outsider (2016), to show how they appropriate and reconfigure notions at the heart of the crisis, such as derivatives, financial trading and markets.
Drawing on ideas from thinkers such as Jonathan Beller, Yves Citton and Peter Szendy, this book shows how derivatives can be taken as a conceptual resource for thinking about creative practice and the circulation of audio-visual images today.
Donate To Help Us Fight Back: Mobilization Rhetoric in Political Fundraising
Authors: Kim Seo-young Silvia , Zilinsky Jan , Brew Brian
Published: March 2023

Political candidates utilize social media to mobilize supporters, persuade voters, and raise money. However, little is known about the structure of mass electoral appeals when donors are the primary target instead of voters. Because candidates' donors and voters can differ significantly, with donors more partisan and ideologically extreme on average, we theorize that candidates use strategic rhetoric tailored to specific audiences. To analyze how campaigns perceive and target their "financial electorate,'' we leverage data from the Facebook Ad Library for 2020 U.S. congressional candidates and distinguish political ads by their persuasion targets. Using text analysis, we test the hypotheses that donor-targeting messages are more toxic, negative, and likely to reference a polarizing president than voter-targeting messages. The results support our hypotheses, and Republican candidates, on average, used more toxic language than their Democratic counterparts. As campaigns' scramble for donations intensifies, these characteristics of fundraising appeals may further polarize the electorate.
Association Between Etiological Factors and Dentin Hypersensitivity: A Cross-Sectional Study in Turkey
Authors: HATİPOĞLU Ömer , PERTEK HATİPOĞLU Fatma
Published: March 2023

Objective: This study purposed to estimate the prevalence of DH and how effective the etiological factors are in the development of DH in Turkey.

Methods: Demographic features, hygiene habits, bruxism, beverage habits, parafunctional habits, smoking, and other medical problems were asked. Air was blasted to the teeth via the air-water syringe for 3 seconds. The Visual Analogue Scale was used to measure DH sensitivity, and data were recorded in the 0-100 mm range, and 5 mm and higher were considered DH. The attrition, abrasion, erosion, abfraction per dental type (Incisal OR Canine OR Premolar OR Molar) were recorded. The same procedure was applied for abnormal tooth position and gingival recession.

Results: A total of 4476 teeth and 236 individuals were evaluated. Significantly higher DH frequency was observed in females (p= .034), the 40- 55 age range (p= .009), and non-smokers (p= .016). Those who brushed their teeth three times a day or more (p< .001), preferred horizontal technique (p= .017), used toothbrushes with a hard bristle (p< .001) exhibited higher DH frequency. There was no significant difference in DH regarding bruxism, acidic beverage consumption, vomiting, and reflux (p> .05).

Conclusion: DH is a multi-etiological symptom affected by demographic attributes, hygiene, and other habits. Clinicians should painstakingly distinguish the source of DH to gain the long-term success of DH treatment, which depends on many etiological factors.
Clinical and Biochemical Effects of Smoking on Non-Surgical Periodontal Treatment in Grade III Stage C Periodontitis Patients
Authors: ARIKAN Volkan , GÖRGÜLÜ Nimet Gül , DOĞAN Başak
Published: March 2023

Objective: The purpose of this study was to evaluate the effect of smoking on clinical parameters and the serum and saliva levels of RANKL, OPG, and IL-34 in periodontitis stage III grade C (III-C) patients after non-surgical periodontal treatment (NSPT).

Methods: A total of 60 subjects, 40 periodontitis-III-C patients (20 smokers and 20 non-smokers) and 20 non-smoker periodontally healthy individuals, were included. All clinical periodontal parameters were recorded, and unstimulated saliva and serum samples were collected from all patients at baseline, but at 1 and 3 months only from periodontitis patients (N=40). Saliva and serum levels of RANKL, OPG, and IL-34 were analyzed by ELISA.

Results: At baseline only whole mouth probing depth (PD) and percent of sites with PD>5mm were higher in smokers than non-smoker periodontitis patients (p
Evaluation of Eating Habits and Nutrient Intake in Adolescents with and without Suspected Eating Disorders in Iran
Authors: MOJANİ QOMİ Mansooreh Sadat , HATAMİ Monireh , ZİARATİ Parisa
Published: March 2023

Objective: Increasing the incidence of eating disorder (ED) attitude during adolescence may lead to inadequate nutrient intake and consequently to growth disorders. This study aimed to compare eating habits and nutrient intakes of adolescents with high risk and low risk of developing eating disorders in high schools in Tehran, Iran.

Methods: In the current cross-sectional study, the Eating Attitude Test (EAT-26) was applied to 299 students (185 females and 114 males) aged 15-18 years for the determination of ED attitudes. Food habits and nutrient intake were assessed by a self-administered questionnaire and a validated Food Frequency Questionnaire (FFQ), respectively. Nutrient adequacy ratio (NAR) and mean adequacy ratio (MAR) were calculated using nutrient’s Dietary Reference Intake (DRI) for age and sex.

Results: Of all students, 17.7% were at risk of developing ED. The results showed that girls, unlike boys, consumed more energy than they needed, but this difference between the ED group in both sexes was not significant. The distribution of sweetened drinks, candy, chocolate, and fast food frequency was significantly different between the two groups of girls (P
Perancangan Sistem Informasi Manajemen Pelatihan Kursus Berbasis Web Pada CV Nixtrain Infotama
Authors: Khamdun Khamdun , Nainggolan Esron Rikardo , Putra Jordy Lasmana
Published: March 2023

Training activities are usually carried out face-to-face, but along with the development of increasingly advanced times, especially in the field of technology, business actors, especially in the field of training, find more efficient and effective ways to run their business or business. The design of a website-based training management information system will make it easier for prospective trainees to access updated training information anywhere and anytime using the internet. Then it will reduce damage and loss of data because data about training activities is stored safely and neatly on a website. The design of this website itself is designed by the methodology used is Object Oriented Programming or commonly abbreviated as OPP.
SISTEM PENERIMAAN SISWA BARU DI SMKN 3 PATI BERDASAR JALUR PRESTASI MENGGUNAKAN ALGORITMA KLASTERING K-MEANS BERBASIS WEB
Authors: Nur Aziz Yassin Achmad , Zuliarso Eri
Published: March 2023

Sistem penerimaan mahasiswa baru yang menggunakan pengelompokan data algoritma K-Means merupakan pola clustering yang paling sederhana dibandingkan dengan algoritma lainnya. Algoritma ini merupakan salah satu data mining. K-Means mengelompokkannya ke dalam beberapa cluster yang memiliki persamaan dan memisahkan setiap cluster berdasarkan perbedaan tiap clusternya.Penelitian algoritma K-Means Clustering bertujuan untuk meminimalkan fungsi-fungsi yang ditetapkan pada saat proses Clustering.Implementasi K-Means Algoritma clustering ke dalam sistem informasi clustering memberikan hasil klasifikasi pengelompokan data yang efektif dan proses setiap rotasi literasi jarak Centroid, penentuan titik Cluster yang terbentuk, data siswa sebagai objek referensi lebih menghemat waktu pada clustering yang unggul kelas. Penerapan sistem informasi clustering berbasis web ini menghasilkan informasi yang lebih fleksibel yang dapat diakses setiap saat oleh pengguna yang diberikan hak akses untuk memanfaatkan data tersebut. Penerapan Algoritma K-Means Clustering untuk mendapatkan hasil klarifikasi Kelas Unggul membutuhkan implementasi sistem informasi untuk membentuk 3 cluster untuk setiap kelas yaitu M1, M2 dan M3. M1 sebagai nilai tinggi dengan nilai kriteria 85 sampai 100, M2 sebagai nilai sedang dengan nilai kriteria 75 sampai 80 dan M3 sebagai nilai rendah dengan nilai kriteria 10 sampai 70.
PERANCANGAN SISTEM INFORMASI TPQ AISYAH MAULIDA HASANAH BERBASIS WEBSITE
Authors: Amirudin Afif , Azrino Gustalika Muhamad
Published: March 2023

Perkembangan dunia digital yang semakin maju membuat kecepatan informasi juga semakin cepat dan kompleks. Namun dengan segala fasilitas yang ada, penggunaan teknologi informasi masih sedikit diterapkan di masyarakat setempat. Proses dokumentasi pembelajaran di TPQ Aisyah Maulidah Hasanah masih menggunakan cara konvensional, dimana siswa dan orang tua siswa hanya dapat melihat hasil belajar melalui media kertas. Pencatatan pembayaran iuran bulanan juga masih menggunakan buku catatan yang berpotensi kehilangan data jika buku hilang diganti atau buku hilang. Teknologi informasi dapat digunakan untuk membantu pihak TPQ Aisyah Maulidah Hasanah dan orang tua siswa agar pencatatan data lebih aman melalui sistem informasi berbasis website yang diharapkan dapat mempermudah dalam pengolahan dan penerimaan informasi oleh pengelola TPQ dan orang tua siswa mengenai perkembangan pencatatan prestasi siswa dan pembayaran iuran. Dengan menggunakan metode waterfall dan framework Laravel, sistem informasi berbasis website ini diharapkan dapat mengefisienkan proses pencatatan informasi terkait perkembangan prestasi siswa dan meminimalisir potensi kehilangan data.
On composite length-biased exponential-Pareto distribution: Properties, simulation, and application in actuarial science
Authors: Benchettah Moulouk Halima , Zeghdoudi Halim , Raman Vinoth
Published: March 2023

The composite length-biased exponential-Pareto (CLBEP) distribution is a new composite distribution that is introduced in this article. This model's probability density function, moments, and quantiles, among other statistical characteristics, are determined mathematically. The parameters' maximum-likelihood estimation and stochastic ordering are discussed. A comparison study with other new composite and conventional distributions is also included. Specifically, using two actual fire insurance data sets, the goodness of fit of this new model is contrasted with the composite exponential-Pareto, composite lognormal-Pareto, and composite Rayleigh-Pareto distributions (Algerian and Danish fire insurance losses).

2010 AMS subject classifications

62E10; 60E05.
Robust fusion for skin lesion segmentation of dermoscopic images
Authors: Guo Qingqing , Fang Xianyong , Wang Linbo , Zhang Enming , Liu Zhengyi
Published: March 2023

Robust skin lesion segmentation of dermoscopic images is still very difficult. Recent methods often take the combinations of CNN and Transformer for feature abstraction and multi-scale features for further classification. Both types of combination in general rely on some forms of feature fusion. This paper considers these fusions from two novel points of view. For abstraction, Transformer is viewed as the affinity exploration of different patch tokens and can be applied to attend CNN features in multiple scales. Consequently, a new fusion module, the Attention-based Transformer-And-CNN fusion module (ATAC), is proposed. ATAC augments the CNN features with more global contexts. For further classification, adaptively combining the information from multiple scales according to their contributions to object recognition is expected. Accordingly, a new fusion module, the GAting-based Multi-Scale fusion module (GAMS), is also introduced, which adaptively weights the information from multiple scales by the light-weighted gating mechanism. Combining ATAC and GAMS leads to a new encoder-decoder-based framework. In this method, ATAC acts as an encoder block to progressively abstract strong CNN features with rich global contexts attended by long-range relations, while GAMS works as an enhancement of the decoder to generate the discriminative features through adaptive fusion of multi-scale ones. This framework is especially good at lesions of varying sizes and shapes and of low contrasts and its performances are demonstrated with extensive experiments on public skin lesion segmentation datasets.
Three-dimensional echocardiography of the tricuspid valve
Authors: Jost Zachary T. , Nooli Nishank P. , Ali Ahmed E. , Jaganathan Vijayadithyan , Nanda Navin C.
Published: March 2023

Due to the proportionally high mortality rates associated with isolated tricuspid valve surgery, the invasive treatment of such pathology, historically, has been left largely unaddressed. Recently, there has been an appreciation for the mortality and morbidity of tricuspid valve disease, giving rise to the movement towards identifying less invasive, transcatheter approaches for treatment. Due to the technical complexity of these procedures along with the uniqueness and variability of tricuspid valve anatomy, a better appreciation of the tricuspid valve anatomy and pathology is required for pre-procedural planning. While two-dimensional echocardiography serves as the initial non-invasive modality for tricuspid valve evaluation, three-dimensional echocardiography provides a complete en face view of the tricuspid valve and surrounding structures, as well contributes further information regarding disease etiology and severity. In this review, we discuss the utility of three-dimensional echocardiography as a supplement to two-dimensional imaging to better assess tricuspid valve disease and anatomy to aide in future innovative therapies.
Case report: CAR-T cell therapy-induced cardiac tamponade
Authors: Sarfati Sacha , Norbert Misa Eugène , Hérault Antoine , Giry Marion , Makké Jade , Grall Maximilien , Savouré Arnaud , Camus Vincent , Alani Mustafa , Tamion Fabienne , Latouche Jean-Baptiste , Girault Christophe
Published: March 2023

CD19-specific chimeric antigen receptor T (CAR-T) cell therapy has recently been shown to improve the prognosis of refractory diffuse large B-cell lymphoma (DLBCL). However, CAR-T cells may induce numerous adverse events, in particular cytokine release syndrome (CRS) which is frequently associated with cardiovascular manifestations. Among the latter, acute pericardial effusion represents less than 1% of cases and cardiac tamponade has only been reported once. The management and outcome of these severe complications are not well established. We report here, a case of cardiac tamponade associated with CRS in a context of CAR-T cell therapy, which required urgent pericardiocentesis.

Case summary

A 65-year-old man with refractory DLBCL was treated with CAR-T cell therapy. He had a history of dilated cardiomyopathy with preserved ejection fraction and transient atrial fibrillation. A pericardial localization of the lymphoma was observed on the second relapse. One day after CAR-T cell infusion the patient was diagnosed with grade 1 CRS. Due to hypotension, he was treated with tocilizumab and dexamethasone, and then transferred to intensive care unit (ICU). Echocardiography performed at ICU admission showed acute pericardial effusion with signs of right ventricular heart failure due to cardiac tamponade. It was decided to perform pericardiocentesis despite grade IV thrombocytopenia in a context of aplasia. Analysis of pericardial fluid showed a large number of lymphoma cells and 73% of CAR-T cells amongst lymphocytes, a level that was similar in blood. Hemodynamic status improved after pericardiocentesis, and no recurrence of pericardial effusion was observed. The presence of a high count of activated CAR-T cells in the pericardial fluid as well as the short interval between CAR-T cells injection and the symptoms appear as potential arguments for a direct action of CAR-T cells in the mechanism of this adverse event. The patient was discharged from ICU after two days and initially exhibited a good response to DLBCL treatment. Unfortunately, he died fifty days after starting CAR-T cell therapy due to a new DLBCL relapse.

Conclusion

Patients with a pericardial localization of DLBCL should be assessed for a risk of cardiac tamponade if receiving CAR-T cell therapy and presenting CRS. In this case, cardiac tamponade seems directly related to CAR-T cell expansion. Pericardiocentesis should be considered as a feasible and effective treatment if the risk of bleeding is well controlled, in association with anti-IL6 and corticosteroids.
Effect of COVID-19 vaccine on blood glucose metrics in Arabic people with type 1 diabetes
Authors: Al-Ozairi Ebaa , Irshad Mohammad , Taghadom Etab , Varghese Anisha , Sojan Litty , Alkandari Jumana
Published: March 2023

Introduction

People with diabetes are at a higher risk for coronavirus disease-19 (COVID-19) and hence are prioritized for vaccination. The aim of the current study was to investigate the effects of COVID-19 vaccination on blood glucose control in Arabic people with type 1 diabetes (T1D). Secondary aim was to compare the responses between the two vaccines approved for use in Kuwait.

Method

This retrospective study compared ambulatory glucose metrics, using a continuous glucose monitoring device, measured for 14 days before, and 7 days and 14 days after, the first and second dose of the COVID-19 vaccine in Arabic people with Type 1 diabetes (T1D). We also explored possible links with vaccine type and other clinical characteristics. Glucose metrics calculated were time in range (TIR, 3.9–10 mmol/L), time above range (TAR, 10.1- 13.9 mmol/L or >13.9 mmol/L), time below range (TBR, 3- 3.9 mmol/L or <3 mmol/L) and glucose variability (CV).

Results

We enrolled 223 participants in the study. Over the 7 days period after the first vaccination dose there was a decrease in TIR (mean difference (SD) –1.9% ± 14.8%; p = 0.05) and increase in TAR >10 mmol/L (2.2% ± 15.9%; p = 0.04), with no effects on TBR. These effects were not seen after the second dose or 14 days after either dose. There was a decrease in CV over the 7 days period after the first (−1.2% ± 7.4%; p = 0.02) and second vaccine doses (−1.1% ± 6.9%; p = 0.03), with no effects noted 14 days after either dose. In subgroup analysis similar effects on TIR and TAR were also seen in those who had received the viral vector-based vaccine, but not the mRNA-based vaccine, although the decrease in CV was seen in those who had received the mRNA based vaccine but not the viral vector-based vaccine.

Conclusion

We found a temporary impairment in glucose control in the first 7 days, particularly among individuals receiving viral vector vaccines. The group receiving mRNA vaccine was likely to experience an increase in glucose levels above the target range. However, the temporary change in metrics appears to return to pre-vaccination levels after one-week post-vaccination. The effects on glycemic parameters were more neutral after the second dose.
Research progress on exosomes in podocyte injury associated with diabetic kidney disease
Authors: Li Jiao , Zheng Shanshan , Ma Chaoqun , Chen Xuexun , Li Xuan , Li Shengjie , Wang Ping , Chen Ping , Wang Zunsong , Li Wenbin , Liu Yipeng
Published: March 2023

Diabetic kidney disease (DKD), a common cause of end-stage renal disease, is a serious complication that develops with the progression of chronic diabetes. Its main clinical manifestations are persistent proteinuria and/or a progressive decline in the estimated glomerular filtration rate. Podocytes, terminally differentiated glomerular visceral epithelial cells, constitute the glomerular filtration barrier together with the basement membrane and endothelial cells, and the structural and functional barrier integrity is closely related to proteinuria. In recent years, an increasing number of studies have confirmed that podocyte injury is the central target of the occurrence and development of DKD, and research on exosomes in podocyte injury associated with DKD has also made great progress. The aim of this review is to comprehensively describe the potential diagnostic value of exosomes in podocyte injury associated with DKD, analyze the mechanism by which exosomes realize the communication between podocytes and other types of cells and discuss the possibility of exosomes as targeted therapy drug carriers to provide new targets for and insights into delaying the progression of and treating DKD.
The role of spermatozoa-zona pellucida interaction in selecting fertilization-competent spermatozoa in humans
Authors: Leung Erica T. Y. , Lee Brayden K. M. , Lee Cheuk-Lun , Tian Xinyi , Lam Kevin K. W. , Li Raymond H. W. , Ng Ernest H. Y. , Yeung William S. B. , Ou Jian-Ping , Chiu Philip C. N.
Published: March 2023

Human fertilization begins when a capacitated spermatozoon binds to the zona pellucida (ZP) surrounding a mature oocyte. Defective spermatozoa-ZP interaction contributes to male infertility and is a leading cause of reduced fertilization rates in assisted reproduction treatments (ARTs). Human ejaculate contains millions of spermatozoa with varying degrees of fertilization potential and genetic quality, of which only thousands of motile spermatozoa can bind to the ZP at the fertilization site. This observation suggests that human ZP selectively interacts with competitively superior spermatozoa characterized by high fertilizing capability and genetic integrity. However, direct evidence for ZP-mediated sperm selection process is lacking. This study aims to demonstrate that spermatozoa-ZP interaction represents a crucial step in selecting fertilization-competent spermatozoa in humans. ZP-bound and unbound spermatozoa were respectively collected by a spermatozoa-ZP coincubation assay. The time-course data demonstrated that ZP interacted with a small proportion of motile spermatozoa. Heat shock 70 kDa protein 2 (HSPA2) and sperm acrosome associated 3 (SPACA 3) are two protein markers associated with the sperm ZP-binding ability. Immunofluorescent staining indicated that the ZP-bound spermatozoa had significantly higher expression levels of HSPA2 and SPACA3 than the unbound spermatozoa. ZP-bound spermatozoa had a significantly higher level of normal morphology, DNA integrity, chromatin integrity, protamination and global methylation when compared to the unbound spermatozoa. The results validated the possibility of applying spermatozoa-ZP interaction to select fertilization-competent spermatozoa in ART. This highly selective interaction might also provide diagnostic information regarding the fertilization potential and genetic qualities of spermatozoa independent of those derived from the standard semen analysis.
Interregional and intersectoral interaction of digital economy in China
Authors: Ma Zhong , Niu Xingxing , Ma Weijing , Kang Yanxia , Xie Weiwei
Published: March 2023

With the continuous advancement of the integration of the world’s digital economy, the economic development of various regions has become increasingly interdependent. However, the interregional interactions of digital industries have yet to be further elucidated. Here, based on the Multi-Regional Input-Output model, we take China, for example, and analyze the impact of digital industries on the economy from the industrial and regional. At the industry level, we discuss the economic spillover relationship between the digital industry and the three traditional industries, and analyzes the spatial and temporal differentiation in the inter-industry spillover status of China’s 30 provinces; at the regional level, we discuss the digital industrial economic spillover links and spillover states. The results show that: 1) The self-generating ability within the digital industry sector is the most significant factor influencing the industrial growth of China’s digital economy, followed by the interrelated effect between industry sectors, while the inter-industry feedback effect has a weak impact on the economic system. 2) There is a clear gap in the economic spillover capacity of the digital industry in 30 provinces, and the overall performance is decreasing in the eastern, central and western regions. 3) The intra-regional multiplier effect of digital industry shows a slowly decreasing trend, and the intra-regional digital economic spillover-feedback effect shows a growing trend. At the same time, the inter-regional digital economy interaction tends to decay in distance, indicating that regional accessibility has a significant impact on the inter-regional spillover effect.
Identification and validation of ferroptosis-related genes and immune cell infiltration in thyroid associated ophthalmopathy
Authors: Chen Sainan , Diao Jiale , Yue Zifan , Wei Ruili
Published: March 2023

Thyroid associated ophthalmopathy (TAO) is an orbital autoimmune inflammatory disease that is commonly associated with thyroid dysfunction. Although the etiology of TAO is unclear, ROS accumulation and oxidative stress have been closely linked to the pathogenesis of TAO. Ferroptosis is an iron-dependent programmed cell death characterized by intracellular labile iron levels, excessive accumulation of reactive oxygen species (ROS) and lipid peroxidation. Currently, there are few reports regarding the role of ferroptosis in TAO. This article aimed to identify ferroptosis-related genes (FRGs) with diagnostic and therapeutic potential in TAO and explore their relationship with immune cells and lncRNAs. GSE58331 was downloaded from Gene Expression Omnibus (GEO) database. A total of 162 DEGs were identified between 27 TAO samples and 22 health samples from GSE58331, among which six FRGs (CYBB, CTSB, SLC38A1, TLR4, PEX3, and ABCC1) were obtained. The AUC of SLC38A1, TLR4, PEX3 in lacrimal gland tissues was greater than 80 which suggested high diagnostic value in TAO. The result of immune cell infiltrate analysis indicated increased infiltration of monocytes (p < 0.001), macrophages M0(p = 0.039), mast cells activated (p = 0.008), and neutrophils (p = 0.045) in orbital tissues from TAO patients. Meanwhile, mast cells resting (p = 0.043) and macrophages M2 (p = 0.02) showed reduced infiltration in TAO samples. There were no gender differences in immune cell infiltration in the TAO patients. Two differentially expressed lncRNAs, LINC01140 and ZFHX4-AS1, in TAO groups were identified as ferroptosis-related lncRNAs. CYBB-LINC01140-TLR4, CYBB- LINC01140- SLC38A1, TLR4- LINC01140- SLC38A1, and CTSB- ZFHX4-AS1- CYBB may be potential RNA regulatory pathways in TAO. Targeted drugs and transcription factors for differential expressed FRGs were also screened out in our study. In vitro, experiments revealed that CTSB, PEX3, ABCC1 and ZFHX4-AS1(lncRNA) were differentially expressed in orbital fibroblasts (OFs) between TAO groups and healthy controls at the transcriptional level.
Congenital insensitivity to pain associated with PRDM12 mutation: Two case reports and a literature review
Authors: Yu Hanrui , Wu Jie , Cong Jinju , Chen Mingxiong , Huang Yifei , Yu Jifeng , Wang Liqiang
Published: March 2023

Background:PRDM12 is a newly discovered gene responsible for congenital insensitivity to pain (CIP). Its clinical manifestations are various and not widely known.

Methods: The clinical data of two infants diagnosed with CIP associated with PRDM12 mutation were collected. A literature review was performed, and the clinical characteristics of 20 cases diagnosed with a mutation of PRDM12 were summarized and analyzed.

Results: Two patients had pain insensitivity, tongue and lip defects, and corneal ulcers. The genomic analysis results showed that variants of PRDM12 were detected in the two families. The case 1 patient carried heterozygous variations of c.682+1G > A and c.502C > T (p.R168C), which were inherited from her father and mother, respectively. We enrolled 22 patients diagnosed with CIP through a literature review together with our cases. There were 16 male (72.7%) and 6 female (27.3%) patients. The age of onset ranged from 6 months to 57 years. The prevalence of clinic manifestation was 14 cases with insensitivity to pain (63.6%), 19 cases with self-mutilation behaviors (86.4%), 11 cases with tongue and lip defects (50%), 5 cases with mid-facial lesions (22.7%), 6 cases with distal phalanx injury (27.3%), 11 cases of recurrent infection (50%), 3 cases (13.6%) with anhidrosis, and 5 cases (22.7%) with global developmental delay. The prevalence of ocular symptoms was 11 cases (50%) with reduced tear secretion, 6 cases (27.3%) with decreased corneal sensitivity, 7 cases (31.8%) with disappeared corneal reflexes, 5.5 cases (25%, 0.5 indicated a single eye) with corneal opacity, 5 cases (22.7%) with corneal ulceration, and 1 case (4.5%) with a corneal scar.

Conclusion: The syndrome caused by PRDM12 mutation is a clinically distinct and diagnosable disease that requires joint multidisciplinary management to control the development of the disease and minimize the occurrence of complications.
Wild-type and mutant p53 in cancer-related ferroptosis. A matter of stress management?
Authors: Corazzari Marco , Collavin Licio
Published: March 2023

Cancer cells within tumor masses are chronically exposed to stress caused by nutrient deprivation, oxygen limitation, and high metabolic demand. They also accumulate hundreds of mutations, potentially generating aberrant proteins that can induce proteotoxic stress. Finally, cancer cells are exposed to various damages during chemotherapy. In a growing tumor, transformed cells eventually adapt to these conditions, eluding the death-inducing outcomes of signaling cascades triggered by chronic stress. One such extreme outcome is ferroptosis, a form of iron-dependent non-apoptotic cell death mediated by lipid peroxidation. Not surprisingly, the tumor suppressor p53 is involved in this process, with evidence suggesting that it acts as a pro-ferroptotic factor and that its ferroptosis-inducing activity may be relevant for tumor suppression. Missense alterations of the TP53 gene are extremely frequent in human cancers and give rise to mutant p53 proteins (mutp53) that lose tumor suppressive function and can acquire powerful oncogenic activities. This suggests that p53 mutation provides a selective advantage during tumor progression, raising interesting questions on the impact of p53 mutant proteins in modulating the ferroptotic process. Here, we explore the role of p53 and its cancer-related mutants in ferroptosis, using a perspective centered on the resistance/sensitivity of cancer cells to exogenous and endogenous stress conditions that can trigger ferroptotic cell death. We speculate that an accurate molecular understanding of this particular axis may improve cancer treatment options.
Using multi-tissue transcriptome-wide association study to identify candidate susceptibility genes for respiratory infectious diseases
Authors: Zhu Xiaobo , Zou Yixin , Jia Linna , Ye Xiangyu , Zou Yanzheng , Tu Junlan , Li Juntong , Yu Rongbin , Yang Sheng , Huang Peng
Published: March 2023

Objective: We explore the candidate susceptibility genes for influenza A virus (IAV), measles, rubella, and mumps and their underlying biological mechanisms.

Methods: We downloaded the genome-wide association study summary data of four virus-specific immunoglobulin G (IgG) level data sets (anti-IAV IgG, anti-measles IgG, anti-rubella IgG, and anti-mumps virus IgG levels) and integrated them with reference models of three potential tissues from the Genotype-Tissue Expression (GTEx) project, namely, whole blood, lung, and transformed fibroblast cells, to identify genes whose expression is predicted to be associated with IAV, measles, mumps, and rubella.

Results: We identified 19 significant genes (ULK4, AC010132.11, SURF1, NIPAL2, TRAP1, TAF1C, AC000078.5, RP4-639F20.1, RMDN2, ATP1B3, SRSF12, RP11-477D19.2, TFB1M, XXyac-YX65C7_A.2, TAF1C, PCGF2, and BNIP1) associated with IAV at a Bonferroni-corrected threshold of p < 0.05; 14 significant genes (SOAT1, COLGALT2, AC021860.1, HCG11, METTL21B, MRPL10, GSTM4, PAQR6, RP11-617D20.1, SNX8, METTL21B, ANKRD27, CBWD2, and TSFM) associated with measles at a Bonferroni-corrected threshold of p < 0.05; 15 significant genes (MTOR, LAMC1, TRIM38, U91328.21, POLR2J, SCRN2, Smpd4, UBN1, CNTROB, SCRN2, HOXB-AS1, SLC14A1, AC007566.10, AC093668.2, and CPD) associated with mumps at a Bonferroni-corrected threshold of p < 0.05; and 13 significant genes (JAGN1, RRP12, RP11-452K12.7, CASP7, AP3S2, IL17RC, FAM86HP, AMACR, RRP12, PPP2R1B, C11orf1, DLAT, and TMEM117) associated with rubella at a Bonferroni-corrected threshold of p < 0.05.

Conclusions: We have identified several candidate genes for IAV, measles, mumps, and rubella in multiple tissues. Our research may further our understanding of the pathogenesis of infectious respiratory diseases.