bluuebunny/crossref_metadata_2025 huggingface dataset:
Therapeutic Keratoplasty for Fusarium Keratitis
Authors: Oliver-Gutierrez David , Gutuleac Liliana , Anglada-Masferrer Natalia , Segura-Duch Gloria , Martin Sara , Bisbe Laia , Martín-Gómez María Teresa , Zapata Miguel Ángel , Puig Javier José
Published: December 2024

Purpose: This study evaluates the effectiveness of therapeutic keratoplasty for Fusarium fungal keratitis and explores the diagnosis and management challenges of this infectious corneal disease. Methods: We retrospectively analyzed therapeutic keratoplasty cases at a tertiary hospital for Fusarium keratitis when standard treatments failed. Results: Five cases of Fusarium keratitis, unresponsive to typical antifungal treatments, required keratoplasty due to fast progression and diagnostic difficulties. Post-surgery, all patients had infection resolution without recurrence, but some complications like anterior chamber leakage, graft rejection, and ocular hypertension were managed effectively. Final visual acuity ranged from 0.9 to finger counting. Conclusions: Fusarium keratitis, often resistant to conventional therapies, may necessitate keratoplasty for resolution. This intervention is crucial for positive outcomes, emphasizing the need for prompt and effective management to prevent severe surgical measures and preserve ocular health.
Serum and Tissue Periostin Expression in Unilateral Benign Lesions of the Nose and the Paranasal Sinuses
Authors: Danielides Gerasimos , Gatsounia Alkmini , Kyriakopoulos George , Stathopoulos Constantinos , Naxakis Stephanos , Lygeros Spyridon
Published: December 2024

Background/Objectives: Periostin’s role in the pathogenesis of inflammatory diseases, particularly in the nose and paranasal sinuses, is an area of growing interest. This study aims to evaluate the expression of periostin in mucoceles, inverted papillomas, choanopolyps and retention cysts. Methods: Tissue samples collected during functional endoscopic sinus surgery (FESS) were analyzed for POSTN gene mRNA expression using qPCR. Periostin protein levels were measured via ELISA and Western Blot. Serum periostin levels were also assessed through ELISA in both patients (n = 54) and controls (n = 12). Results: A total of 66 participants were recruited, including 18 with inverted papillomas, 10 with mucoceles, 10 with choanopolyps, 16 with retention cysts and 12 controls. There were no significant alternations between tissue and serum samples of inverted papilloma compared to the control group. Choanopolyp tissues exhibited elevated POSTN protein expression, though POSTN mRNA and serum levels remained unchanged. In mucoceles, periostin levels were significantly elevated in both tissues and serum. Retention cyst tissues demonstrated an increase in POSTN mRNA and protein expression, whereas serum periostin levels remained consistent with those observed in the control group. Conclusions: The findings suggest that periostin may play a role in the pathophysiology of benign non-neoplastic lesions of the nose and paranasal sinuses such as mucoceles, retention cysts and choanopolyps, highlighting a need for more investigation in this subject.
Variability of Suspended Sediment Runoff as an Expression of the Long-Term Dynamics of Loess Gully Development—An Example from the Lublin Upland (Eastern Poland)
Authors: Rodzik Jan , Janicki Grzegorz , Stępniewski Krzysztof , Kociuba Waldemar
Published: December 2024

In the 20-year period from 2003 to 2022, water runoff and suspended sediment load in a forested gully system with a total length of 7.5 km was recorded. The branching gully system cuts through an agricultural catchment of 1.24 km2 located within the loess plateau of the Nałęczów Plateau (E Poland). A rain gauge was installed close to the watershed and in the gully mouth water runoff was monitored using a limnigraph, installed with a water gauge on a Thomson triangular weir. To determine suspended sediment concentrations during erosion episodes, water was sampled at a frequency dependent on the rate and duration of the runoff. The aim of the monitoring studies was to specify the relationship between flow rate and suspended concentration for different water runoff conditions, which were used to calculate sediment load. The contribution of propluvial and pronivial runoff to gully development was assessed, particularly in the transport of sediment out of the catchment, and unit denudation rates were calculated as less than 1000 Mg km−2 year−1, average 173 Mg km−2 year−1, and during a single event as a maximal 900 Mg km−2.
The Evolution Relationship Between Intensive Land Use and Land Ecological Security in the Urban Agglomeration in the Northern Slope of the Tianshan Mountains, Northwest China
Authors: Zhu Xianwei , Ye Jianming , Zhu Mengmeng , Gao Zhe , Li Miaomiao , Wang Mei , Li Yingbin
Published: December 2024

Revealing the relationship between intensive land use (ILU) and land ecological security (LES) is crucial for achieving high-quality land resource development and ensuring the sustainability of land ecosystems, especially in arid regions with fragile human–land relationships. In this study, we evaluated ILU and LES in the urban agglomeration on the northern slope of the Tianshan Mountains (UANSTM) and analyzed the evolution of ILU and LES using Pearson correlation analysis, four-quadrant model, and coupling coordination degree model. The results indicated that from 2012 to 2022, ILU and its three subsystems generally showed an upward trend in the UANSTM, with an average annual growth rate of 2.96%, 2.50%, 3.32%, and 0.289%, respectively, and with ILU levels significantly higher in the eastern and central counties compared to other areas. LES and its three subsystems also exhibited a general increase, with average annual growth rates of 1.63%, 0.86%, 3.10%, and 1.51%, respectively, although high-level areas displayed distinct spatial variations. The Pearson correlation analysis showed that the land input level had a significant negative effect on the pressure, but the positive effects of state and response enhanced the LES. The number of double-high cities increased significantly from 1 to 6, and the number of double-low cities decreased from 6 to 0. Compared with other cities and counties, the LES-lagging counties and cities faced the challenge of coordinating urban development and ecological conservation. While coupling coordination levels across all counties improved to varying degrees, none reached the optimal coordination stage. The findings and methodologies of this study provide helpful insights into human–land relationships in arid regions, supporting the goals of high-quality urban development and sustainability.
Comparative Biomechanical Analysis of Kirschner Wire Fixation in Dorsally Displaced Distal Radius Fractures
Authors: Dmour Awad , Toma Ștefan-Lucian , Cazac Alin-Marian , Tirnovanu Stefan Dragos , Dima Nicoleta , Dmour Bianca-Ana , Popescu Dragos Cristian , Alexa Ovidiu
Published: December 2024

Objective: This study aims to evaluate and compare the biomechanical performance of two Kirschner (K) wire configurations—the intra-focal and interfragmentary techniques—for the fixation of dorsally displaced distal radius fractures. The study also assesses the impact of K-wire diameter (1.6 mm vs. 2.0 mm) on mechanical stability. Methods: Sixty fresh turkey tarsometatarsus bones were selected and divided into four groups based on the K-wire configuration and diameter used. Fractures were created at standardized locations, and each bone was stabilized using either the intra-focal also known as modified Kapandji (Ka) or interfragmentary technique. Mechanical testing, including axial compression and flexion tests, was performed to assess the biomechanical stability of each configuration. Results: The interfragmentary configuration consistently demonstrated superior biomechanical performance compared to the intra-focal technique. Specifically, the use of 2.0 mm K-wires resulted in significantly higher axial stiffness (13.28 MPa) and load at break (3070 N) compared to the 1.5 mm wires. Confidence intervals further supported the robustness of these findings. The interfragmentary technique, especially with thicker K-wires, provided greater load-bearing capacity and stiffness. Conclusion: The interfragmentary technique with 2.0 mm K-wires offers superior mechanical stability compared to the intra-focal technique, making it the preferred choice for stabilizing comminuted extra-articular distal radius fractures. These findings suggest that adopting this technique may reduce the risk of postoperative complications such as fracture displacement or malunion. Further research involving osteoporotic bone models and clinical trials is recommended to validate these findings in real-world settings.
A Study on the Effect of Graphene Oxide on Geotechnical Properties of Soil
Authors: Park Kyungwon , Kim Ju-Hoon , Shin Junwoo , Lee Hoyoung , Nam Boo Hyun
Published: December 2024

Edge-oxidized graphene oxide (EOGO) is a nano-sized material that is chemically stable and easily mixed with water due to its hydrophilic properties; thus, it has been used in various engineering fields, particularly for the reinforcement of building and construction materials. In this study, the effect of EOGO in soil reinforcement was investigated. When mixed with soil, it affects the mechanical properties of the soil–GO mixture. Various amounts of the GO (0%, 0.02%, 0.06%, 0.1%) were added into the sand–clay mixture, and their geotechnical properties were evaluated via multiple laboratories testing methods, including a standard Proctor test, direct shear test, compressibility test, and contact angle measurement. The experimental results show that with the addition of EOGO in soil of up to 0.06% EOGO, the compressibility decreases, the shear strength increases, and the maximum dry density (after compaction) increases.
Biomechanics of Osseointegration of a Dental Implant in the Mandible Under Shock Wave Therapy: In Silico Study
Authors: Smolin Alexey , Eremina Galina , Martyshina Irina , Xie Jing
Published: December 2024

The most time-consuming aspect of dental prosthesis installation is the osseointegration of a metal implant with bone tissue. The acceleration of this process may be achieved through the use of extracorporeal shock wave therapy. The objective of this study is to investigate the conditions for osseointegration of the second premolar implant in the mandibular segment through the use of a poroelastic model implemented in the movable cellular automaton method. The mandibular segment under consideration includes a spongy tissue layer, 600 µm in thickness, covered with a cortical layer, 400 µm in thickness, and a gum layer, 400 µm in thickness. Furthermore, the periodontal layers of the roots of the first premolar and first molar were considered, while the implant of the second premolar was situated within a shell of specific tissue that corresponded to the phase of osseointegration. The model was subjected to both physiological loading and shock wave loading across the three main phases of osseointegration. The resulting fields of hydrostatic pressure and interstitial fluid pressure were then subjected to analysis in accordance with the mechanobiological principles. The results obtained have indicated that low-intensity shock wave therapy can accelerate and promote direct osseointegration: 0.05–0.15 mJ/mm2 in the first and second phases and less than 0.05 mJ/mm2 in the third phase. In comparison to physiological loads (when bone tissue regeneration conditions are observed only around the implant distal end), shock waves offer the primary advantage of creating conditions conducive to osseointegration along the entire surface of the implant simultaneously. This can significantly influence the rate of implant integration during the initial osteoinduction phase and, most crucially, during the longest final phase of bone remodeling.
The Use of Microwave Treatment as a Sustainable Technology for the Drying of Metallurgical Sludge
Authors: Ślęzak Marta , Migas Piotr , Bernasowski Mikolaj
Published: December 2024

The modern metallurgical industry produces approximately 90% of the volume of all produced steel; for this, integrated technology based on fossil materials such as coal, fluxes, and especially iron ore is used. This industry generates large amounts of waste and by-products at almost all stages of production. Alternative iron and steel production technologies based on iron ore, methane, or pure hydrogen are also not waste-free. To ensure sustainable waste management, efforts are made to seal processes as well as capture and recycle dusty waste. This work presents the results of research on the processing of sludge resulting from the dedusting of the basic oxygen furnace (BOF) process and landfilling in a lagoon. The work discusses the treatment of fine dusty sludge hydrated to 26–60% H2O, to which various amounts of caking agents were added; also discussed are the rheological characteristics of the tested suspension systems, the possibility of forming these systems into larger fractions, and rapid drying using 100–600 W microwaves with a drying time of 1–9 min. The aim was to identify, describe, and characterize the parameters of the agglomeration process and obtain a product that was durable enough to transport and dose into slag baths in order to reduce iron oxides in liquid phases. During the research, completely dried briquettes with an appropriate strength were obtained. The study demonstrates that microwave drying at 300 W for 6 min achieved complete drying with a weight loss of 35%, whereas a higher-power treatment at 750 W for 2 min enhanced compressive strength by up to 95% and reached 15 N/psc, which was comparable with green iron ore pellets. This approach offers a sustainable alternative to traditional methods, but with a reduced drying time.
Atmospheric Corrosion of Different Steel Types in Urban and Marine Exposure
Authors: Paterlini Luca , Brenna Andrea , Ceriani Federica , Gamba Matteo , Ormellese Marco , Bolzoni Fabio
Published: December 2024

The aim of the present work is to study the atmospheric corrosion behavior of metals exposed to both urban (Milan, IT-Lombardia) and marine (Bonassola, IT-Liguria) atmospheres in Italy. A number of coupons (100 × 150 mm) of carbon steel (CS), hot-dip galvanized steel (GS) and different grades of stainless steel (SS) were exposed. At fixed periods of time, samples were characterized by means of Linear Polarization Resistance (LPR), mass loss tests and corrosion product analysis. The corrosion rate on carbon steel exposed to an urban atmosphere, obtained by means of mass loss tests and LPR, are in good agreement with the value estimated by the dose–response function according to the ISO 9223 standard. The yielded results can be classified in corrosivity class C2 of the same ISO 9223. Similar measurements on galvanized steel exhibited a coherent average corrosion rate. Higher corrosion rates were measured for samples exposed to a marine atmosphere for both materials, with values belonging to exposure classes C4-C5 for both materials. Stainless steel samples exhibited only superficial staining in the case of marine exposure, even after just a few months.
A Study of the Response Surface Methodology and Finite Element Method to Model Aircraft Engine Body Deformations During Hole Drilling
Authors: Matras Andrzej , Machno Magdalena
Published: December 2024

The aviation industry is still looking for effective manufacturing methods. Currently, the challenge is still the machining of shapes in thin-walled materials. This work focuses on the analysis of the influence of these parameters on deformations during the drilling process of holes in the adapters of aircraft engine body accessories. The drilling process was carried out in the thin-walled superalloy Inconel 718, which is classified as a difficult-to-machine material. During the analyses, experimental studies based on the Response Surface Method (RSM) and finite element method (FEM) calculations were carried out simultaneously. The aim of this work was to analyze the developed mathematical models describing nonlinear relationships between cutting parameters, cutting forces, and deformations of the aircraft engine body characterized by a complex, thin-walled geometric structure. By using the proposed solutions, it is possible to achieve integration between the techniques of conducting research, performing calculations using FEM, and designing the machining. The advantage of this comprehensive approach used in our work is the development of mathematical models that strongly fit the results of the research. The results of analyses and calculations presented in the article and the research methodology used can be applied to industrial conditions.
Research on Structural Optimization and Excitation Control Method Using a Two-Dimensional OWPT System for Capsule Robots Based on Non-Equivalent Coils
Authors: Li Wenwei , Jiang Pingping , Wang Zhiwu , Yan Guozheng
Published: December 2024

The rapid development of wireless power transfer (WPT) technology has provided new avenues for supplying continuous and stable power to capsule robots. In this article, we propose a two-dimensional omnidirectional wireless power transfer (OWPT) system, which enables power to be transmitted effectively in multiple spatial directions. This system features a three-dimensional transmitting structure with a Helmholtz coil and saddle coil pairs, combined with a one-dimensional receiving structure. This design provides sufficient internal space, accommodating patients of various body types. Based on the magnetic field calculation and finite element analysis, the saddle coil structure is optimized to enhance magnetic field uniformity; to achieve a two-dimensional rotating magnetic field, a phase difference control method for the excitation signal is developed through the analysis of circuit topology and quantitative synthesis of non-equivalent magnetic field vectors. Finally, an experimental prototype is built, and the experimental results show that the one-dimensional transmitting coil achieves a minimum received voltage stability of 94.5% across different positions. When the three-dimensional transmitting coils operate together, a two-dimensional rotating magnetic field in the plane is achieved at the origin, providing a minimum received power of 550 mW with a voltage fluctuation rate of 7.68%.
Late Paleozoic Tectonics of the NW Tarim Block: Insights from Zircon Geochronology and Geochemistry in Xinjiang, China
Authors: Yang Baozhong , Lv Ao , Zhang Xiangrong , Zhou Yejin , Zhou Wenxiao , Chi Fru Ernest
Published: December 2024

The Late Paleozoic strata on the northwestern margin of the Tarim Block provide valuable insights into the subduction and collision processes that formed the Southwest Tianshan Orogenic Belt. This study integrates detrital zircon U-Pb dating and sandstone geochemical analysis of the Balikelike and Kalundaer formations to examine sedimentary provenance and tectonic settings during the Cisuralian–Guadalupian Epoch in the Keping area on the northwestern margin of the Tarim Block. Three of five Precambrian detrital zircon U-Pb age populations, 2500–2300 and 2000–1800 Ma and 900–600 Ma, are likely related to the fragmentation of the Columbia supercontinent and Rodinia’s assembly, respectively. Two Paleozoic detrital zircons, 500–380 Ma, are associated with Paleozoic magmatism. Among them, ~295 Ma zircons are associated with post-collisional extension and emplacement of the Tarim Large Igneous Province. Geochemical analysis of sandstones, coupled with tectonic reconstruction, indicates a passive continental margin setting in the northwestern margin of the Tarim Block during the Silurian Period, later transitioned to a foreland basin from the Pennsylvanian to the Guadalupian Epochs. The crustal transformation from the Middle-late Devonian to Early Mississippian marked the closure of the South Tianshan Ocean (STO), involving a soft collision and significant uplift, with major orogenesis occurring in the Late Guadalupian. Five key stages are identified in the evolution of the foreland basin: (1) Middle-late Devonian to Early Mississippian initiation (remnant ocean basin stage); (2) Late Mississippian to Early Pennsylvanian early stage; (3) Late Pennsylvanian to Early Cisuralian middle stage; (4) the Late Cisuralian stage; and (5) the terminal Guadalupian stage. These findings provide new constraints on when STO closed and propose an innovative foreland basin evolution model during the late post-collisional phase from the Late Mississippian to Guadalupian. Collectively, the data advance our understanding of the tectonic processes that shaped the northwestern Tarim Block, with broader implications for Paleozoic geodynamics.
The Influence of Basal Medium on Polyphenol Accumulation in Shoot Cultures of Clerodendrum trichotomum and Clerodendrum colebrookianum
Authors: Gomulski Jan , Krzemińska Marta , Jochymek Magdalena , Kiss Anna K. , Grzegorczyk-Karolak Izabela
Published: December 2024

Plants of the Clerodendrum genus, known for their rich phytochemical profiles, are used in traditional Chinese, Korean, Japanese, and Indian medicine to treat various ailments, including inflammation, hypertension, diabetes, hyperlipidemia, and cancer. Due to the limited natural availability of these plants, there is a growing interest in utilizing in vitro culture techniques to produce their bioactive compounds sustainably. In this study, the effects are compared of Murashige and Skoog (MS), Woody Plant medium (WP), Gamborg B5 (B5), and Schenk and Hildebrandt (SH) basal media on growth, biomass accumulation, and polyphenolic compound production in shoot cultures of Clerodendrum colebrookianum and Clerodendrum trichotomum. The composition of the culture medium significantly influenced the growth and metabolic profiles of both species. C. trichotomum exhibited the highest proliferation potential on WP and SH media, while C. colebrookianum was similar on WP, SH, and B5 media (multiplication factor of about 20). Dry weight accumulation was highest in C. trichotomum grown on SH medium (0.292 g/culture), while C. colebrookianum achieved a comparable biomass on SH and WP media (0.240 g/culture and 0.228 g/culture, respectively). The chemical analysis showed similar secondary metabolite profiles between the two Clerodendrum species with phenylethanoids such as acteoside being the predominant bioactive compounds in hydromethanolic extracts. WP medium was the most favorable for polyphenol accumulation in C. colebrookianum (64.5 mg/g DW), while the SH medium yielded the highest total polyphenol content in C. trichotomum (36.6 mg/g DW). In this study, the importance is underscored of basal medium selection in optimizing the in vitro production of bioactive polyphenolic compounds in Clerodendrum species, providing a foundation for the sustainable and scalable production of these pharmacologically significant metabolites.
Advances in Nanotheranostic Systems for Concurrent Cancer Imaging and Therapy: An Overview of the Last 5 Years
Authors: Lankoff Anna Małgorzata , Czerwińska Malwina , Kruszewski Marcin
Published: December 2024

The rapid development of nanotechnology during the last two decades has created new opportunities to design and generate more advanced nanotheranostics with diversified capabilities for diagnosis, drug delivery, and treatment response monitoring in a single platform. To date, several approaches have been employed in order to develop nanotheranostics. The purpose of this review is to briefly discuss the key components of nanotheranostic systems, to present the conventional and upcoming imaging and therapeutic modalities that employ nanotheranostic systems, and to evaluate recent progress in the field of cancer nanotheranostic systems in the past five years (2020–2024). Special attention is focused on the design of cancer nanotheranostic systems, their composition, specificity, potential for multimodal imaging and therapy, and in vitro and in vivo characterization.
The Anti-AGEing and RAGEing Potential of Isothiocyanates
Authors: Krisanits Bradley A. , Kaur Bhoomika , Fahey Jed W. , Turner David P.
Published: December 2024

Isothiocyanates (ITCs), found in edible plants such as cruciferous vegetables, are a group of reactive organo-sulfur phytochemicals produced by the hydrolysis of precursors known as glucosinolates. ITCs have been studied extensively both in vivo and in vitro to define their therapeutic potential for the treatment of chronic health conditions. Therapeutically, they have shown an intrinsic ability to inhibit oxidative and inflammatory phenotypes to support enhanced health. This review summarizes the current evidence supporting the observation that the antioxidant and anti-inflammatory activities of ITCs temper the pathogenic effects of a group of reactive metabolites called advanced glycation end products (AGEs). AGE exposure has significantly increased across the lifespan due to health risk factors that include dietary intake, a sedentary lifestyle, and comorbid conditions. By contributing to a chronic cycle of inflammatory stress through the aberrant activation of the transmembrane receptor for AGE (RAGE), increased AGE bioavailability is associated with chronic disease onset, progression, and severity. This review debates the potential molecular mechanisms by which ITCs may inhibit AGE bioavailability to reduce RAGE-mediated pro-oxidant and pro-inflammatory phenotypes. Bringing to light the molecular impact that ITCs may have on AGE biogenesis may stimulate novel intervention strategies for reversing or preventing the impact of lifestyle factors on chronic disease risk.
Controllable Synthesis of Three-Dimensional Chiral Au Nanoflowers Induced by Cysteine with Excellent Biocompatible Properties
Authors: Liu Shengmiao , Zhang Jianhao , Yan Wenjing
Published: December 2024

Chiral molecules are ubiquitous in nature and biological systems, where the unique optical and physical properties of chiral nanoparticles are closely linked to their shapes. Synthesizing chiral plasmonic nanomaterials with precise structures and tunable sizes is essential for exploring their applications. This study presents a method for growing three-dimensional chiral gold nanoflowers (Au NFs) derived from trisoctahedral (TOH) nanocrystals using D-cysteine and L-cysteine as chiral inducers. By employing a two-step seed-mediated growth approach, stable chiral Au nanoparticles with customizable sizes, shapes, and optical properties were produced by adjusting the Au nanosphere (Au NP) seed concentration and cysteine dosage. These nanoparticles exhibited optical activity in both the visible and near-infrared regions, with a maximum anisotropy factor (g-factor) of 0.024. Furthermore, the PEG-modified chiral Au NFs demonstrated excellent biocompatibility. This approach provides a precise method for geometrically controlling the design of three-dimensional chiral nanomaterials, holding great potential for biomedical applications.
Study of Thermalization Mechanisms of Hot Carriers in BABr-Added MAPbBr3 for the Top Layer of Four-Junction Solar Cells
Authors: Zhang Yi , Chen Huilong , Qu Junfeng , Zhang Jiayu , Conibeer Gavin
Published: December 2024

The hot carrier multi-junction solar cell (HCMJC) is an advanced-concept solar cell with a theoretical efficiency greater than 65%. It combines the advantages of hot carrier solar cells and multi-junction solar cells with higher power conversion efficiency (PCE). The thermalization coefficient (Qth) has been shown to slow down by an order of magnitude in low-dimensional structures, which will significantly improve PCE. However, there have been no studies calculating the Qth of MAPbBr3 quantum dots so far. In this work, the Qth values of MAPbBr3 quantum dots and after BABr addition were calculated based on power-dependent steady-state photoluminescence (PD-SSPL). Their peak positions in PD-SSPL increased from 2.37 to 2.71 eV after adding BABr. The fitting shows that, after adding BABr, the Qth decreased from 2.64 ± 0.29 mW·K−1·cm−2 to 2.36 ± 0.25 mW·K−1·cm−2, indicating a lower relaxation rate. This is because BABr passivates surface defects, slowing down the carrier thermalization process. This work lays the foundation for the theoretical framework combining perovskite materials, which suggests that the appropriate passivation of BABr has the potential to further reduce Qth and make MAPbBr3 QDs with BABr modified more suitable as the top absorption layer of HCMJCs.
The Role of Breast Milk Cell-Free DNA in the Regulation of the Neonatal Immune Response
Authors: Rezai Tamim , Fell-Hakai Shani , Guleria Shalini , Toldi Gergely
Published: December 2024

The neonatal period is a critical phase for the development of the intestinal immune system, marked by rapid adaptation to the external environment and unique nutritional demands. Breast milk plays a pivotal role in this transition, yet the mechanisms by which it influences neonatal mucosal immunity remain unclear. This review examines the potential mechanisms by which cell-free DNA (cfDNA) in breast milk may impact neonatal immune development, particularly through Toll-like receptor 9 (TLR9) signalling and gut microbiota interactions. We propose that cfDNA in breast milk interacts with TLR9 on the apical surface of neonatal intestinal epithelial cells, potentially serving as an initial anti-inflammatory stimulus before the establishment of commensal bacteria. This hypothesis is supported by the high concentration and stability of cfDNA in breast milk, as well as the known activation of TLR9 by mitochondrial DNA in breast milk. The review emphasises the need for further empirical research to validate these interactions and their implications for neonatal health, suggesting that understanding these dynamics could lead to improved strategies for neonatal care and disease prevention.
Effects of the Ketogenic Diet on Microbiota Composition and Short-Chain Fatty Acids in Women with Overweight/Obesity
Authors: Güzey Akansel Müge , Baş Murat , Gençalp Cansu , Kahrıman Meryem , Şahin Eray , Öztürk Hakan , Gür Gürsel , Gür Ceren
Published: December 2024

Background/Objectives: The ketogenic diet (KD) is a dietary model that can impact metabolic health and microbiota and has been widely discussed in recent years. This study aimed to evaluate the effects of a 6-week KD on biochemical parameters, gut microbiota, and fecal short-chain fatty acids (SCFAs) in women with overweight/obesity. Methods: Overall, 15 women aged 26–46 years were included in this study. Blood samples, fecal samples, and anthropometric measurements were evaluated at the beginning and end of this study. Results: After KD, the mean body mass index decreased from 29.81 ± 4.74 to 27.12 ± 4.23 kg/m2, and all decreases in anthropometric measurements were significant (p < 0.05). Fasting glucose, insulin, homeostasis model assessment of insulin resistance, hemoglobin A1C, urea, and creatinine levels decreased, whereas uric acid levels increased (p < 0.05). Furthermore, increased serum zonulin levels were noted (p = 0.001), whereas fecal butyrate, propionate, acetate, and total SCFA levels decreased (p < 0.05). When the changes in microbiota composition were examined, a decrease in beta diversity (p = 0.001) was observed. After the intervention, a statistically significant increase was noted in the Firmicutes/Bacteroidetes ratio (p = 0.001). Although Oscilibacter, Blautia, and Akkermensia relative abundances increased, Prevotella relative abundance and Bifidobacter abundance, which were the dominant genera before the KD, decreased. Moreover, the abundance of some pathogenic genera, including Escherichia, Klebsilella, and Listeria, increased. Conclusions: In healthy individuals, KD may cause significant changes in microbial composition, leading to dysbiosis and long-term adverse outcomes with changes in serum zonulin and fecal SCFA levels.
Folic Acid Prevents High-Fat Diet-Induced Postpartum Weight Retention in Rats, Which Is Associated with a Reduction in Endoplasmic Reticulum Stress-Mediated Hepatic Lipogenesis
Authors: Zhang Huaqi , Zhang Li , Zhao Xuenuo , Ma Yanzhen , Sun Dan , Bai Yixian , Liu Weiheng , Liang Xi , Liang Hui
Published: December 2024

Background: Proactively preventing postpartum weight retention (PPWR) is one of the effective intervention strategies to reduce the occurrence of obesity in women. Population studies have shown that serum folate levels are closely related to body weight. The regulation of folic acid on lipid metabolism has been fully confirmed in both in vivo and in vitro studies. For many years, folic acid supplementation has been widely used in periconceptional women due to its role in preventing fetal neural tube defects. However, whether folic acid supplementation prior to and throughout pregnancy exerts preventive effects on PPWR remains uncertain. This study aims to investigate the preventive effect of folic acid on PPWR in rats and further explore the underlying mechanisms. Methods: In this study, pregnant rats were administered one of the dietary schedules: control diet (CON), high-fat diet (HF), control diet combined with folic acid (FA) and high-fat diet combined with folic acid (HF + FA). Results: We discovered that folic acid supplementation inhibited high-fat diet-induced elevations in body weight, visceral fat weight, liver weight, hepatic lipid levels and serum lipid levels at 1 week post-weaning (PW). Western blot analysis showed that folic acid supplementation inhibited the expression of endoplasmic reticulum (ER) stress-specific proteins including GRP78, PERK, eIF2α, IRE1α, XBP1 and ATF6, subsequently decreasing the expression of proteins related to lipid synthesis including SREBP-1c, ACC1 and FAS. Conclusions: In conclusion, folic acid supplementation prior to and throughout pregnancy exerts preventive effects on high-fat diet-induced PPWR in rats, and the mechanism is associated with the inhibition of ER stress-mediated lipogenesis signaling pathways in the liver. Folic acid supplementation may serve as a potential strategy for preventing PPWR. In the future, the effectiveness of folic acid in PPWR prevention can be further verified by population studies.
Acute Co-Ingestion of Caffeine and Sodium Bicarbonate on Muscular Endurance Performance
Authors: Montalvo-Alonso Juan Jesús , Munilla César , Garriga-Alonso Laura , Ferragut Carmen , Valadés David , Gonzalo-Encabo Paola , Pérez-López Alberto
Published: December 2024

Background: Caffeine and sodium bicarbonate individually enhance muscular endurance by delaying fatigue, but their combined effects have scarcely been studied. Objectives: This study aimed to evaluate the acute effects of co-ingesting caffeine and sodium bicarbonate on muscular endurance at different loads in bench press and back squat exercises. Methods: Twenty-seven recreationally trained participants (female/male: 14/14; age: 23 ± 3.6 years) were randomized to four conditions in a double-blind, crossover design: (a) sodium bicarbonate and caffeine (NaHCO3 + CAF); (b) sodium bicarbonate (NaHCO3); (c) caffeine (CAF); (d) placebo (PLA); ingesting 0.3 g/kg NaHCO3, 3 mg/kg caffeine or placebo (maltodextrin). Participants performed two muscle endurance tests on bench press and back squat exercises at 65% and 85% 1RM, performing as many repetitions as possible in one set until task failure. Results: CAF increased the number of repetitions (p < 0.001; ηp2 = 0.111), mean velocity (Vmean, p = 0.043, ηp2 = 0.16), and mean power output (Wmean, p = 0.034, ηp2 = 0.15) compared to placebo. These effects were observed in back squat exercise at 65%1RM in Vmean (3.7%, p = 0.050, g = 1.144) and Wmean (5.2%, p = 0.047, g = 0.986) and at 85%1RM in Vmean (5.4%, p = 0.043, g = 0.22) and Wmean (5.5%, p = 0.050, g = 0.25). No ergogenic effects were found in NaHCO3 + CAF) or NaHCO3 conditions. Conclusions: CAF increased muscular endurance performance in male and female participants by increasing the number of repetitions, mean velocity, and power output; however, when NaHCO3 was ingested, these effects were not detected.
Building of CuO2@Cu-TA@DSF/DHA Nanoparticle Targets MAPK Pathway to Achieve Synergetic Chemotherapy and Chemodynamic for Pancreatic Cancer Cells
Authors: Zhang Jiaru , Li Zuoping , Xie Zhenzhen , You Shiwan , Chen Yanbing , Zhang Yuling , Zhang Jing , Zhao Na , Deng Xiling , Sun Shiguo
Published: December 2024

Background/Objectives: With the increase of reactive oxygen species (ROS) production, cancer cells can avoid cell death and damage by up-regulating antioxidant programs. Therefore, it will be more effective to induce cell death by using targeted strategies to further improve ROS levels and drugs that inhibit antioxidant programs. Methods: Considering that dihydroartemisinin (DHA) can cause oxidative damage to protein, DNA, or lipids by producing excessive ROS, while, disulfiram (DSF) can inhibit glutathione (GSH) levels and achieve the therapeutic effect by inhibiting antioxidant system and amplifying oxidative stress, they were co-loaded onto the copper peroxide nanoparticles (CuO2) coated with copper tannic acid (Cu-TA), to build a drug delivery system of CuO2@Cu-TA@DSF/DHA nanoparticles (CCTDD NPs). In response to the tumor microenvironment, DHA interacts with copper ion (Cu2+) to produce ROS, and a double (diethylthiocarbamate)-copper (II) (CuET) is generated by the complexation of DSF and Cu2+, which consumes GSH and inhibits antioxidant system. Meanwhile, utilizing the Fenton-like effect induced by the multi-copper mode can achieve ROS storm, activate the MAPK pathway, and achieve chemotherapy (CT) and chemodynamic (CDT). Results: Taking pancreatic cancer cell lines PANC-1 and BxPC-3 as the research objects, cell line experiments in vitro proved that CCTDD NPs exhibit efficient cytotoxicity on cancer cells. Conclusions: The CCTDD NPs show great potential in resisting pancreatic cancer cells and provides a simple strategy for designing powerful metal matrix composites.
Environmental Factors Influencing Species Richness Expression in Grasslands of the Colombian Orinoquia
Authors: Minorta-Cely Vladimir , Niño Larry , Rangel Orlando , Sánchez-Mata Daniel
Published: December 2024

The relationships between environmental characteristics and species richness in the grasslands of the Colombian Orinoquia are presented and analyzed using an ordinal logistic regression model. Ordinal and scale covariates were included, and their bivariate significance was assessed using Spearman’s rho and Kendall’s Tau-b. The covariates that showed statistical significance with the weighted richness thresholds (WRT) and defined the model were the soil depth and the soil moisture regime, both of which had positive correlations. In contrast, the percentage of bare soil and the monthly minimum temperature showed negative correlations. This contribution highlights the relevance of articulating and combining the floristic and phytosociological characterization of grassland vegetation to advance the predictive studies aimed at defining and understanding the potential divergent relationships between the vegetation and the basic attributes of the natural environment, along with their implications for conservation.
Gully Erosion Susceptibility Prediction Using High-Resolution Data: Evaluation, Comparison, and Improvement of Multiple Machine Learning Models
Authors: Li Heyang , Jin Jizhong , Dong Feiyang , Zhang Jingyao , Li Lei , Zhang Yucheng
Published: December 2024

Gully erosion is one of the significant environmental issues facing the black soil regions in Northeast China, and its formation is closely related to various environmental factors. This study employs multiple machine learning models to assess gully erosion susceptibility in this region. The primary objective is to evaluate and optimize the top-performing model under high-resolution UAV data conditions, utilize the optimized best model to identify key factors influencing the occurrence of gully erosion from 11 variables, and generate a local gully erosion susceptibility map. Using 0.2 m resolution DEM and DOM data obtained from high-resolution UAVs, 2,554,138 pixels from 64 gully and 64 non-gully plots were analyzed and compiled into the research dataset. Twelve models, including Logistic Regression, K-Nearest Neighbors, Classification and Regression Trees, Random Forest, Boosted Regression Trees, Adaptive Boosting, Extreme Gradient Boosting, an Artificial Neural Network, a Convolutional Neural Network, as well as optimized XGBOOST, a CNN with a Multi-Head Attention mechanism, and an ANN with a Multi-Head Attention Mechanism, were utilized to evaluate gully erosion susceptibility in the Dahewan area. The performance of each model was evaluated using ROC curves, and the model fitting performance and robustness were validated through Accuracy and Cohen’s Kappa statistics, as well as RMSE and MAE indicators. The optimized XGBOOST model achieved the highest performance with an AUC-ROC of 0.9909, and through SHAP analysis, we identified roughness as the most significant factor affecting local gully erosion, with a relative importance of 0.277195. Additionally, the Gully Erosion Susceptibility Map generated by the optimized XGBOOST model illustrated the distribution of local gully erosion risks.
Modal Analysis with Asymptotic Strips Boundary Conditions of Skewed Helical Gratings on Dielectric Pipes as Cylindrical Metasurfaces for Multi-Beam Holographic Rod Antennas
Authors: Ng Mou Kehn Malcolm , Lin Ting-Wei , Chen Wei-Chuan
Published: December 2024

A core dielectric cylindrical rod wrapped in a dielectric circular pipe whose outer surface is enclosed by a helical conducting strip grating that is skewed along the axial direction is herein analyzed using the asymptotic strip boundary conditions along with classical vector potential analysis. Targeted for use as a cylindrical holographic antenna, the resultant field solutions facilitate the aperture integration of the equivalent cylindrical surface currents to obtain the radiated far fields. As each rod section of a certain skew angle exhibits a distinct modal attribute; this topology allows for the distribution of the cylindrical surface impedance via the effective refractive index to be modulated, as in gradient-index (GRIN) materials. Beam steering can also be achieved by altering the skew angle via mechanical sliding motion while leaving the cylindrical structure itself unchanged, as opposed to impractically reconfiguring the geometrical and material parameters of the latter to attain each new beam direction. The results computed by the program code based on the proposed technique in terms of the modal dispersion and radiation patterns are compared with simulations by a software solver. Manufactured prototypes are measured, and experimentally acquired dispersion diagrams and radiation patterns are favorably compared with theoretical predictions.
Developing a Theoretical Framework of Export-Oriented Small Enterprises: A Multiple Case Study in an Emerging Country
Authors: Chaldun Evy Rachmawati , Yudoko Gatot , Prasetio Eko Agus
Published: December 2024

Small enterprises are essential in supporting economic growth, particularly in emerging countries. Due to their constrained resources and capacities, many small businesses in developing nations encounter intricate obstacles when trying to enter the global market rapidly. The study is intended to develop a theoretical framework that can reveal the essential and integrated resources, and capabilities in the internationalization process. Instead of literature investigations, multiple case studies were adopted to explore the process of achieving the international success of Indonesia’s export-oriented small enterprises. In-depth interviews with twelve small enterprises across the culinary, fashion, and craft sectors were conducted to collect qualitative data. A content analysis followed the input–process–output–outcome structure as the basis for developing the robust framework. Based on the Resource-Based View (RBV), this study reveals the synergistic role of production, networks, marketing, learning, and legal capabilities in creating competitive advantages that support business continuity and sustainability. Research findings reveal that successful internationalization is not achieved by a single capability but through an integrated bundle of capabilities that can serve market needs. This study contributes to the literature by offering a comprehensive framework that maps the input–process–output–outcome structure of the internationalization process and offers practical insights for policymakers and practitioners aiming to enhance SME competitiveness. The results underscore the importance of capability development and government support in facilitating SME global expansion. Ultimately, this study provides a basis for further investigation into the dynamic capabilities that SMEs need to thrive in international markets.
Measuring Urban Environmental Awareness: Instrument Validation and Analysis in Urban Contexts
Authors: Baltodano-Nontol Luz , Alvarado-Silva Carlos , Fernández-Mantilla Mirtha , Gálvez-Carrillo Rosa , Acevedo-Duque Ángel
Published: December 2024

Environmental and climate change issues are a consequence of human actions that, whether consciously or unconsciously, damage the environment. The main objective of this research is to develop an instrument to measure environmental awareness levels among citizens. The developed questionnaire consists of 34 items, adapted and grouped into four dimensions: affective, cognitive, conative, and active. The main distinction of this questionnaire from prior models is its aim to assess environmental awareness levels applicable to any citizen. The scale’s reliability is demonstrated by high internal consistency (α = 0.965 and Ω = 0.958). The key findings on scale performance are evident in the scale’s validity, supported by a confirmatory factor analysis that shows a KMO index of 0.981, a significant Bartlett’s test of sphericity (p < 0.01), and a matrix determinant below 0.001. The maximum likelihood model shows an adequate fit (p < 0.01) and identifies four factors, which together explain 70.134% of the total variance: the first factor contributes 53.075%, the second 12.097%, the third 2.962%, and the fourth 2%. An important finding is the instrument’s high consistency and robustness for evaluating environmental awareness in citizens, offering a reliable and specific tool for environmental studies in urban contexts.
Determinants of the Implementation of a Circular Economy Model in the Tire Sector in Poland
Authors: Nowaczek Agnieszka , Kowalski Zygmunt , Kulczycka Joanna , Makara Agnieszka
Published: December 2024

This work discusses the drivers and barriers (legal, chemical, biological, technical) related to implementing a circular economy model in the tire sector in Poland. The aim of this work was to identify and prioritize key factors determining the implementation of the circular economy business model, both among tire manufacturers and recyclers. The analysis of aspects facilitating the implementation of circular economy and the assessment of this type of project in organizations was the basis for identifying the most important factors in the development of eco-innovation in the tire industry. The research process used bibliometric analysis, empirical research based on a questionnaire and in-depth interviews of tire industry employees, and opinions of experts participating in a group interview. Research has indicated that the key to success in implementing the circular economy is to change actual business practices. The basic drivers of circular economy implementation in the tire sector are legal requirements and an organization’s profit associated with the use of circular economy actions. High environmental requirements and regulations that support a circular economy are key factors stimulating eco-innovative methods, but in most cases, these are more of a concern for recyclers than tire manufacturers. This study identified and hierarchized the determinants for implementing a circular economy model and indicated key factors influencing the development of eco-innovations among recyclers and tire manufacturers in Poland.