bluuebunny/crossref_metadata_2025 huggingface dataset:
Theranostic 64Cu-DOTHA2-PSMA allows low toxicity radioligand therapy in mice prostate cancer model
Authors: Milot Marie-Christine , Bélissant-Benesty Ophélie , Dumulon-Perreault Véronique , Ait-Mohand Samia , Geha Sameh , Richard Patrick O. , Rousseau Étienne , Guérin Brigitte
Published: January 2023

Introduction

We have previously shown that copper-64 (64Cu)-DOTHA2-PSMA can be used for positron emission tomography (PET) imaging of prostate cancer. Owing to the long-lasting, high tumoral uptake of 64Cu-DOTHA2-PSMA, the objective of the current study was to evaluate the therapeutic potential of 64Cu-DOTHA2-PSMA in vivo.

Methods

LNCaP tumor-bearing NOD-Rag1nullIL2rgnull (NRG) mice were treated with an intraveinous single-dose of 64Cu-DOTHA2-PSMA at maximal tolerated injected activity, natCu-DOTHA2-PSMA at equimolar amount (control) or lutetium-177 (177Lu)-PSMA-617 at 120 MBq to assess their impact on survival. Weight, well-being and tumor size were followed until mice reached 62 days post-injection or ethical limits. Toxicity was assessed through weight, red blood cells (RBCs) counts, pathology and dosimetry calculations.

Results

Survival was longer with 64Cu-DOTHA2-PSMA than with natCu-DOTHA2-PSMA (p < 0.001). Likewise, survival was also longer when compared to 177Lu-PSMA-617, although it did not reach statistical significance (p = 0.09). RBCs counts remained within normal range for the 64Cu-DOTHA2-PSMA group. 64Cu-DOTHA2-PSMA treated mice showed non-pathological fibrosis and no other signs of radiation injury. Human extrapolation of dosimetry yielded an effective dose of 3.14 × 10-2 mSv/MBq, with highest organs doses to gastrointestinal tract and liver.

Discussion

Collectively, our data showed that 64Cu-DOTHA2-PSMA-directed radioligand therapy was effective for the treatment of LNCaP tumor-bearing NRG mice with acceptable toxicity and dosimetry. The main potential challenge is the hepatic and gastrointestinal irradiation.
Associations between CES1 variants and dosing and adverse effects in children taking methylphenidate
Authors: Brown Jacob T. , Beery Nancy , Taran Allise , Stevens Tyler , Henzler Christine , Badalamenti Jonathan , Regal Ron , McCarty Catherine A.
Published: January 2023

Background

Methylphenidate is the most prescribed stimulant to treat attention deficit-hyperactivity disorder (ADHD). Despite its widespread usage, a fair proportion of children are classified as non-responders to the medication. Variability in response and occurrence of adverse events with methylphenidate use may be due to several factors, including drug-drug interactions as well as pharmacogenetic differences resulting in pharmacokinetic and/or pharmacodynamic variances within the general population. The objective of this study was to analyze the effect of carboxylesterase 1 (CES1) variants on the frequency of adverse effects and dosing requirements of methylphenidate in children with ADHD.

Methods

This was a retrospective cohort study of children and adolescents who met the inclusion criteria and had a routine visit during the enrollment period were invited to participate. Inclusion criteria included: ADHD diagnosis by a healthcare provider, between 6 and 16 years of age at the time of permission/assent, had not previously been prescribed methylphenidate, and treatment with any methylphenidate formulation for at least three consecutive months. Three months of records were reviewed in order to assess changes in dose and frequency of discontinuing methylphenidate. Participants’ ADHD symptoms, medication response, adverse effects, select vitals, and dose were extracted from the electronic health record. Saliva samples were collected by trained study coordinators. Haplotypes were assigned based on copy number in different portions of the CES1 gene. Due to limited numbers, diplotypes (combinations of two haplotypes) were grouped for analysis as CES1A1/CES1A1, CES1A1/CES1A1c and CES1A1c/CES1A1c.

Results

A total of 99 participants (n = 30 female; n = 69 male) had both clinical data and CES1 sequencing data, with an average age of 7.7 years old (range 3–15 years). The final weight-based dose in all individuals was 0.79 mg/kg/day. The most common adverse effects reported were decreased appetite (n = 47), weight loss (n = 24), and sleep problems (n = 19). The mean final weight-based dose by haplotype was 0.92 mg/kg for CES1A2/CES1A2, 0.81 mg/kg for CES1A2/CES1P1, and 0.78 mg/kg for CES1P1/CES1P1. After correction for multiple hypothesis testing, only one SNV, rs114119971, was significantly associated with weight-based dosing in two individuals. The individuals with the rs114119971 SNV had a significantly lower weight-based dose (0.42 mg/kg) as compared to those without (0.88 mg/kg; p < 0.001).

Discussion

Variation in CES1 activity may impact dose requirements in children who are prescribed methylphenidate, as well as other CES1 substrates. Although intriguing, this study is limited by the retrospective nature and relatively small sample size.
Effectiveness of plyometric training vs. complex training on the explosive power of lower limbs: A Systematic review
Authors: Wang Xiaolin , Lv Changhai , Qin Xinmin , Ji Shuyu , Dong Delong
Published: January 2023

Introduction: Explosive power is considered an important factor in competitive events. Thus, strategies such as complex training (CT) and plyometric training (PLT) are effective at improving explosive power. However, it is still not clear which of the two strategies can enable greater improvements on the explosive power. Thus, the aim of this systematic review was to compare the effects of PLT and CT on the explosive power of the lower limbs.

Methods: The Review Manager and GraphPad Prism programs were used to analyze the synthetic and time effects (effects over training time) on explosive power (i.e., jump ability, sprint ability) and maximum strength. Our research identified 87 studies comprising 1,355 subjects aged 10–26.4 years.

Results: The results suggested the following: 1) Synthetic effects on jump ability (Hedges’ g): .79 (p < .001) for unloaded PLT, 1.35 (p < .001) for loaded PLT and .85 (p < .001) for CT; 2) Synthetic effects on sprint ability: .83 (p < .001) for unloaded PLT, −2.11 (p < .001) for loaded PLT and −.78 (p < .001) for CT; 3) Synthetic effects on maximum strength: .84 (p < .001) for loaded PLT and 1.53 (p < .001) for CT; 4) The time effects of unloaded PLT and CT on explosive power were similar, but the time effects of CT on maximum strength were obviously above that of PLT.

Discussion: In conclusion, unloaded PLT and CT have a similar effect on explosive performance in the short term but loaded PLT has a better effect. The improvement of the maximum strength caused by CT was greater than that induced by PLT. In addition, more than 10 weeks of training may be more beneficial for the improvement of power. Therefore, for explosive power training, we suggest adopting unloaded or light-loaded PLT during a short season and applying CT during an annual or long training cycle.
Integrated metabolomics and lipidomics evaluate the alterations of flavor precursors in chicken breast muscle with white striping symptom
Authors: Kong Fuli , Bai Lu , He Zhengxiao , Sun Jiahong , Tan Xiaodong , Zhao Di , Feng Furong , Liu Dawei , Zhao Guiping , Wen Jie , Liu Ranran
Published: January 2023

White striping (WS) is the most common myopathy in the broiler chicken industry. To reveal flavor changes of WS meat objectively, flavor precursors of WS breast muscle were evaluated systematically with integrated metabolomics and lipidomics. The results showed that WS could be distinguished from normal controls by E-nose, and four volatile compounds (o-xylene, benzene, 1,3-dimethyl, 2-heptanone and 6-methyl and Acetic acid and ethyl ester) were detected as decreased compounds by gas chromatography-mass spectrometry. Lipidomic analysis showed that WS breast fillets featured increased neutral lipid (83.8%) and decreased phospholipid molecules (33.2%). Targeted metabolomic analysis indicated that 16 hydrophilic metabolites were altered. Thereinto, some water-soluble flavor precursors, such as adenosine monophosphate, GDP-fucose and L-arginine increased significantly, but fructose 1,6-bisphosphate and L-histidine significantly decreased in the WS group. These results provided a systematic evaluation of the flavor precursors profile in the WS meat of broiler chickens.
Comparison of body composition components and anaerobic performance parameters of elite male motorcycle speedway riders between pre- and post-competitive season
Authors: Michalik Kamil , Szczepan Stefan , Markowski Maciej , Zatoń Marek
Published: January 2023

The purpose of this study was to compare body composition components and anaerobic capacity indices in elite motorcycle speedway riders before and after the competitive season. This study included 12 volunteer male motorcycle speedway riders from the highest speedway league in Poland (PGE Speedway Ekstraliga) (age: 22.7 ± 6.0 years, body height: 171.3 ± 4.0 cm, body weight: 62.5 ± 3.1 kg). Before and after the competitive season, an assessment of body composition and Wingate test (WAnT) on a cycle ergometer with analysis of acute cardiorespiratory and biochemical responses was conducted. Sport level for all riders was defined by the number of heats won (WS), the total number of points scored in all heats including bonus points (PTS + B), and the percentage of heats won (%W). The motorcycle speedway riders participated in an average of 75.3 ± 15.0 total heats (HS) and obtained PTS + B equal 136.8 ± 48.2. The anaerobic performance, body composition, cardiorespiratory and biochemical responses did not changed after the competitive season. The significant statistical correlations were noted between the differences in the Fatigue Index during WAnT and total number of heats (r = −0.61) and with PTS + B (r = −0.58). Positively correlations were observed also between HS and differences: fat mass (FM) (r = 0.60) and percentage of fat mass (r = 0.61) (all p < 0.05). These findings reveal that the HS during the competitive season is related to the stabilization of anaerobic capacity in the WAnT. The HS is positively correlated with an increase in fat mass, and additional fat mass can adversely affect athletic performance in speedway.
Transcriptome and QTL mapping analyses of major QTL genes controlling glucosinolate contents in vegetable- and oilseed-type Brassica rapa plants
Authors: Kim Jin A. , Moon Heewon , Kim Hyang Suk , Choi Dasom , Kim Nan-Sun , Jang Juna , Lee Sang Woo , Baskoro Dwi Nugroho Adji , Kim Dong-Hwan
Published: January 2023

Glucosinolates (GSLs) are secondary metabolites providing defense against pathogens and herbivores in plants, and anti-carcinogenic activity against human cancer cells. Profiles of GSLs vary greatly among members of genus Brassica. In this study, we found that a reference line of Chinese cabbage (B. rapa ssp. pekinensis), ‘Chiifu’ contains significantly lower amounts of total GSLs than the oilseed-type B. rapa (B. rapa ssp. trilocularis) line ‘LP08’. This study aimed to identify the key regulators of the high accumulation of GSLs in Brassica rapa plants using transcriptomic and linkage mapping approaches. Comparative transcriptome analysis showed that, in total, 8,276 and 9,878 genes were differentially expressed between ‘Chiifu’ and ‘LP08’ under light and dark conditions, respectively. Among 162 B. rapa GSL pathway genes, 79 were related to GSL metabolism under light conditions. We also performed QTL analysis using a single nucleotide polymorphism-based linkage map constructed using 151 F5 individuals derived from a cross between the ‘Chiifu’ and ‘LP08’ inbred lines. Two major QTL peaks were successfully identified on chromosome 3 using high-performance liquid chromatography to obtain GSL profiles from 97 F5 recombinant inbred lines. The MYB-domain transcription factor gene BrMYB28.1 (Bra012961) was found in the highest QTL peak region. The second highest peak was located near the 2-oxoacid-dependent dioxygenase gene BrGSL-OH.1 (Bra022920). This study identified major genes responsible for differing profiles of GSLs between ‘Chiifu’ and ‘LP08’. Thus, our study provides molecular insights into differences in GSL profiles between vegetative- and oilseed-type B. rapa plants.
Fusion of hyperspectral imaging (HSI) and RGB for identification of soybean kernel damages using ShuffleNet with convolutional optimization and cross stage partial architecture
Authors: Zheng Ling , Zhao Mingyue , Zhu Jinchen , Huang Linsheng , Zhao Jinling , Liang Dong , Zhang Dongyan
Published: January 2023

Identification of soybean kernel damages is significant to prevent further disoperation. Hyperspectral imaging (HSI) has shown great potential in cereal kernel identification, but its low spatial resolution leads to external feature infidelity and limits the analysis accuracy. In this study, the fusion of HSI and RGB images and improved ShuffleNet were combined to develop an identification method for soybean kernel damages. First, the HSI-RGB fusion network (HRFN) was designed based on super-resolution and spectral modification modules to process the registered HSI and RGB image pairs and generate super-resolution HSI (SR-HSI) images. ShuffleNet improved with convolution optimization and cross-stage partial architecture (ShuffleNet_COCSP) was used to build classification models with the optimal image set of effective wavelengths (OISEW) of SR-HSI images obtained by support vector machine and ShuffleNet. High-quality fusion of HSI and RGB with the obvious spatial promotion and satisfactory spectral conservation was gained by HRFN. ShuffleNet_COCSP and OISEW obtained the optimal recognition performance of ACCp=98.36%, Params=0.805 M, and FLOPs=0.097 G, outperforming other classification methods and other types of images. Overall, the proposed method provides an accurate and reliable identification of soybean kernel damages and would be extended to analysis of other quality indicators of various crop kernels.
Identification of PLATZ genes in Malus and expression characteristics of MdPLATZs in response to drought and ABA stresses
Authors: Sun Yaqiang , Liu Yunxiao , Liang Jiakai , Luo Jiawei , Yang Fan , Feng Peien , Wang Hanyu , Guo Bocheng , Ma Fengwang , Zhao Tao
Published: January 2023

Plant AT-rich sequences and zinc-binding proteins (PLATZ) play crucial roles in response to environmental stresses. Nevertheless, PLATZ gene family has not been systemically studied in Rosaceae species, such as in apple, pear, peach, or strawberry. In this study, a total of 134 PLATZ proteins were identified from nine Rosaceae genomes and were classified into seven phylogenetic groups. Subsequently, the chromosomal localization, duplication, and collinearity relationship for apple PLATZ genes were investigated, and segmental duplication is a major driving-force in the expansion of PLATZ in Malus. Expression profiles analysis showed that PLATZs had distinct expression patterns in different tissues, and multiple genes were significantly changed after drought and ABA treatments. Furthermore, the co-expression network combined with RNA-seq data showed that PLATZ might be involved in drought stress by regulating ABA signaling pathway. In summary, this study is the first in-depth and systematic identification of PLATZ gene family in Rosaceae species, especially for apple, and provided specific PLATZ gene resource for further functional research in response to abiotic stress.
Invented spelling in English and pinyin in multilingual L1 and L2 Cantonese Chinese speaking children in Hong Kong
Authors: Zhou Yanling , McBride Catherine
Published: January 2023

This research examined the relations among Cantonese phonological awareness, invented spelling in Pinyin (in Mandarin), and invented English spelling in 29 first language (L1) and 34 s language (L2) Cantonese-speaking second and third graders in Hong Kong. The purpose of this study was to understand how phonological awareness skills across languages are associated in multilinguals. We compared the phonological skills in the two groups (i.e., L1 and L2 Chinese speaking children) for the three official languages (i.e., Cantonese, Mandarin, and English) spoken in Hong Kong. The two groups did not differ on Cantonese phonological awareness, Mandarin Pinyin invented spelling, or English invented spelling, but the L1 group performed significantly better than the L2 group on Mandarin Pinyin tone skills, with non-verbal intelligence and grade level statistically controlled. In both groups, all three of the phonological sensitivity measures were significantly correlated with one another. With group, grade, and nonverbal IQ statistically controlled, only Mandarin Pinyin invented spelling but not Cantonese phonological awareness uniquely explained English invented spelling performance. In contrast, Pinyin invented spelling was uniquely explained by both English invented spelling and Cantonese phonological awareness skills. Results highlight some phonological transfer effects across languages.
Parentification, distress, and relationship with parents as factors shaping the relationship between adult siblings and their brother/sister with disabilities
Authors: Levante Annalisa , Martis Chiara , Del Prete Cristina Maria , Martino Paola , Pascali Ferruccio , Primiceri Patrizia , Vergari Massimo , Lecciso Flavia
Published: January 2023

According to parentification theory, when the siblings of a brother/sister with disabilities assume parent-like duties, this role reversal is known as sibling-focused parentification. It has a significant impact on these siblings’ distress and the quality of their family relationships; 605 Italian adult siblings (19–26 years) of people with disabilities completed the online survey. Measures of siblings’ parentification, distress, quality of family relationships, social support, and perceived benefits of parentification were used. The hypothesized model aims to test, on the target sample, the distress and the quality of the relationship with parents as mediators on the interplay between the siblings’ parentification and their sibling relationship. Additionally, social support and perceived benefits of parentification as protective factors were considered. Results showed that the distress and the low quality of the relationship with parents negatively affected the interplay between the siblings’ parentification and the relationship with their own brother/sister with disabilities. Social support and the perceived benefits of parentification decreased the siblings’ distress levels; the perceived benefits of parentification served as a protective factor for the quality of the relationship with parents. Current findings extend the knowledge regarding the risk and protective factors of the siblings’ mental health when disability occurs in the family. Additionally, they inform family-based intervention programs, which should involve the whole family system for reducing distress and improving the wellbeing of siblings without disabilities.
Compulsory care of individuals with severe substance use disorders and alcohol- and drug-related mortality: A Swedish registry study
Authors: Scarpa Simone , Grahn Robert , Lundgren Lena M.
Published: January 2023

Aim

This study used 17 year of Swedish registry data (2003–2019) for 25,125 adults assessed for their severity of substance use to identify the baseline factors predicting the risk of being court-ordered into compulsory care and examine the association between admission to compulsory care and mortality risks due to alcohol- or drug-related causes.

Methods and materials

Addiction Severity Index (ASI) assessment data were linked to register data on demographic characteristics, compulsory care, and alcohol- and drug-related mortality. Cox regression models were used to identify baseline factors predictive of post-assessment admission to compulsory care in the 5 years post-substance use assessment. Discrete-time random-effect logistic regression models were used to examine the association between compulsory care duration and alcohol or drug-related mortality risks. Propensity score matching was used for validation.

Results

The first models identified that younger age, female gender, and ASI composite scores for drug use, mental health and employment were significantly associated with the risk of placement in compulsory care for drugs other than alcohol. Female gender and ASI composite scores for alcohol, drug use and employment were significantly associated with compulsory care treatment for alcohol use. The second models showed that older individuals and men were more likely to die due to alcohol-related causes, while younger individuals and men were more likely to die due to drug-related causes. Length of stay in compulsory care institutions significantly increased the likelihood of dying due to substance use-related causes. Propensity scores analyses confirmed the results.

Conclusion

In Sweden, a significant concern is the higher likelihood of women and young individuals to be court-ordered to compulsory care. Although compulsory care is often advocated as a life-saving intervention, our findings do not provide strong support for this claim. On the contrary, our findings show that admission to compulsory care is associated with a higher risk of substance use-related mortality. Factors such as compulsory care often not including any medical or psychological therapy, together with relapse and overdose after discharge, may be possible contributing factors to these findings.
Association between sleep duration and quality with rapid kidney function decline and development of chronic kidney diseases in adults with normal kidney function: The China health and retirement longitudinal study
Authors: Xu Sujuan , Jin Jifu , Dong Qi , Gu Chenjie , Wu Yong , Zhang Haibo , Yin Yingchao , Jia Huiyang , Lei Mingcheng , Guo Junfei , Xu Haixia , Chang Suchi , Zhang Feng , Hou Zhiyong , Zhang Liping
Published: January 2023

Research have shown that sleep is associated with renal function. However, the potential effects of sleep duration or quality on kidney function in middle-aged and older Chinese adults with normal kidney function has rarely been studied. Our study aimed to investigate the association of sleep and kidney function in middle-aged and older Chinese adults. Four thousand and eighty six participants with an eGFR ≥60 ml/min/1.73 m2 at baseline were enrolled between 2011 and 2015 from the China Health and Retirement Longitudinal Study. Survey questionnaire data were collected from conducted interviews in the 2011. The eGFR was estimated from serum creatinine and/or cystatin C using the Chronic Kidney Disease Epidemiology Collaboration equations (CKD-EPI). The primary outcome was defined as rapid kidney function decline. Secondary outcome was defined as rapid kidney function decline with clinical eGFR of <60 ml/min/1.73 m2 at the exit visit. The associations between sleep duration, sleep quality and renal function decline or chronic kidney disease (CKD) were assessed based with logistic regression model. Our results showed that 244 (6.0%) participants developed rapid decline in kidney function, while 102 (2.5%) developed CKD. In addition, participants who had 3–7 days of poor sleep quality per week had higher risks of CKD development (OR 1.86, 95% CI 1.24–2.80). However, compared with those who had 6–8 h of night-time sleep, no significantly higher risks of rapid decline in kidney function was found among those who had <6 h or >8 h of night time sleep after adjustments for demographic, clinical, or psychosocial covariates. Furthermore, daytime nap did not present significant risk in both rapid eGFR decline or CKD development. In conclusion, sleep quality was significantly associated with the development of CKD in middle-aged and older Chinese adults with normal kidney function.
A feasibility study of a new instrument for detection of autism signs in preschool settings. Psychometric properties of the joint attention observation schedule preschool (JA-OBS preschool)
Authors: Linnsand Petra , Hermansson Jonas , Gillberg Christopher , Nygren Gudrun
Published: January 2023

Background

Identifying signs of autism is essential for enabling timely diagnosis and intervention. Children from multiethnic and socioeconomic disadvantaged settings are typically diagnosed with autism later than their general peers. This feasibility study explored the psychometric properties of a new instrument, Joint Attention Observation Schedule Preschool (JA-OBS Preschool), in children with suspected autism.

Methods

Data were collected from a prospective longitudinal study of 46 children aged 2–4 years who lived in a multiethnic, low resource area. The children had been referred from the Child Health Centre to a multiprofessional team for a neuropsychiatric assessment. In the diagnostic process, the instrument JA-OBS Preschool for observation of a child's capability of engaging in joint attention was included. Descriptive statistics and Cronbach's alfa were used to analyze the psychometric properties, including internal consistency reliability and inter-rater consistency.

Results

All 46 children with suspected autism screened positive on the JA-OBS Preschool. The internal consistency reliability of the instrument was calculated at 0.8 (Cronbach's alfa). Percent agreement between two examiners in JA-OBS Preschool ranged from 77% to 100%.

Conclusion

JA-OBS Preschool was found to be a promising instrument for identifying signs of autism in children in this setting. However, further research is needed to determine the psychometric properties of the JA-OBS Preschool in a general child population as well as in a younger age group.
Intervening for sustainable change: Tailoring strategies to align with values and principles of communities
Authors: Metz Allison , Kainz Kirsten , Boaz Annette
Published: January 2023

This paper presents a rationale for tailoring implementation strategies within a values-driven implementation approach. Values-driven implementation seeks to organize implementers around clarifying statements of their shared values in ways that harmonize implementation dynamics related to individual and group mental models, relationships among implementers, and the implementation climate. The proposed approach to tailoring strategies is informed by systems theory and emphasizes the need to focus on both tangible events and behaviors, as well deeper patterns, structures, relationships, and mental models, in order to increase the likelihood of sustaining implementation efforts and improving outcomes for people and communities. We offer for consideration three specific sets of context determinants that are under-represented in the implementation literature and that emerge as especially relevant within a systems approach to identifying and successfully tailoring implementation strategies in the implementation setting including relationships, mental models, and implementation climate.
Latitudes and land use: Global biome shifts in vegetation persistence across three decades
Authors: Southworth Jane , Ryan Sadie J. , Herrero Hannah V. , Khatami Reza , Bunting Erin L. , Hassan Mehedy , Muir Carly S. , Waylen Peter
Published: January 2023

Introduction: The dynamics of terrestrial vegetation are shifting globally due to environmental changes, with potential repercussions for the proper functioning of the Earth system. However, the response of global vegetation, and the variability of the responses to their changing environment, is highly variable. In addition, the study of such changes and the methods used to monitor them, have in of themselves, been found to significantly impact the findings.

Methods: This research builds on a recently developed vegetation persistence metric, which is simple to use, is user‐controlled to assess levels of statistical significance, and is readily reproducible, all designed to avoid these potential pitfalls. This study uses this vegetation persistence metric to present a global exploration of vegetation responses to climatic, latitudinal, and land‐use changes at a biomes level across three decades (1982–2010) of seasonal vegetation activity via the Normalized Difference Vegetation Index (NDVI).

Results: Results demonstrated that positive vegetation persistence was found to be greater in June, July, August (JJA), and September, October, November (SON), with an increasing vegetation persistence found in the Northern Hemisphere (NH) over the Southern Hemisphere (SH). While vegetation showed positive persistence overall, this was not constant across all studied biomes. Overall forested biomes along with mangroves showed positive responses towards enhanced vegetation persistence in both the northern hemisphere and southern hemisphere. Contrastingly, desert, xeric shrubs, and savannas exhibited no significant persistence patterns, but the grassland biomes showed more negative persistence patterns and much higher variability over seasons, compared to the other biomes. The main drivers of changes appear to relate to climate, with tropical biomes linking to the availability of seasonal moisture, whereas the northern hemisphere forested biomes are driven more by temperature. Grasslands respond to moisture also, with high precipitation seasonality driving the persistence patterns. Land-use change also affected biomes and their responses, with many biomes having been significantly impacted by humans such that the vegetation response matched land use and not biome type.

Discussion: The use here of a novel statistical time series analysis of NDVI at a pixel level, and looking historically back in time, highlights the utility and power of such techniques within global change studies. Overall, the findings match greening trends of other research but within a finer scale both temporally and spatially which is a critical new development in understanding global vegetation shifts.
Construction, validation and, visualization of a web-based nomogram to identify the best candidates for primary tumor resection in advanced cutaneous melanoma patients
Authors: Li Zhehong , Wei Junqiang , Zheng Honghong , Zhang Yafang , Zhang Yange , Cao Haiying , Jin Yu
Published: January 2023

Background

Existing studies have shown whether primary site resection (PSR) in cutaneous melanoma (CM) patients with stage IV is controversial. Our study aimed to identify the clinical characteristics of CM patients with stage IV who benefited from PSR on a population-based study.

Methods

We retrospectively reviewed stage IV CM patients in the Surveillance, Epidemiology, and End Results (SEER) database from 2004 to 2015. Patients were divided into surgical and non-surgical groups according to whether PSR was performed or not. According to the median cancer-specific survival (CSS) time of the non-surgery group, the surgical group was divided into the surgery-benefit group and the non-surgery-benefit group. Multivariate cox regression analysis was used to explore independent CSS prognostic factors in the surgical group. Then, based on the independent prognostic factors of the surgical group, we established a web-based nomogram based on logistics regression.

Results

A total of 574 stage IV CM patients were included in our study, and 491 (85.60%) patients were included in the surgical group. The clinical characteristics (benefit group and non-benefit group) included age, M stage, lesion location, and ulceration status. These independent prognostic factors were includeed to construct a web-based nomogram.

Conclusions

We constructed a web-based nomogram. This model was suitable for identifying the best candidates suitable for PSR in stage IV CM patients.
Assessments of productive performance, eggshell quality, excreta moisture, and incubation traits of laying breeder hens fed a proprietary blend of Quillaja and Yucca
Authors: Souza Otoniel F. , Adams Carine B. , Agilar Jessica C. , Biselo Valeria , Mello Renius O. , Gomez Luis G. , Bonaspetti Sandra , Stefanello Catarina
Published: January 2023

A study was conducted to evaluate performance, eggshell quality, nutrient metabolizability, and incubation traits of laying breeder hens fed diets supplemented with an additive containing polyphenols and saponins of a proprietary blend from Quillaja saponaria and Yucca schidigera (QY) biomass. Hens were fed 4 feeds in 5 periods of 28 days each from 30 to 49 weeks of age. Experimental feeds were a Control diet; Control + virginiamycin (33 g/ton); Control + QY (250 g/ton) and Control + virginiamycin + QY. A total of 40 White Plymouth Rock and 44 Rhode Island Red breeder hens were allocated in individual cages using a completely randomized block design with 21 replicates. Performance parameters, evaluated per period, were egg production, egg weight, FCR, egg mass, and culled eggs. All eggs were collected in the last 4 days of each period to evaluate specific egg weight, percentage of albumen, yolk and shell, and Haugh unit as well as cuticle quality, shell strength, and shell thickness. At the end of the experiment, nutrient metabolizability assessment and four incubations were conducted. There were no interactions between diet and period in all evaluated responses (P > 0.05). Experimental diets did not affect daily egg production, egg weight, and egg mass as well as Haugh unit, yolk and albumen percentage, and yolk color (P > 0.05). However, hens fed Control + QY produced eggs with better shell strength, shell thickness, and cuticle quality than hens fed the Control (P < 0.05). Hens fed Control + QY or Control + virginiamycin + QY had lower culled eggs, better FCR and higher egg specific weight, shell percentage, and yolk strength compared to breeder hens fed the Control (P < 0.05). In general, hens fed QY achieved enhanced performance and egg quality compared to virginiamycin. In conclusion, laying breeder hens fed diets supplemented with Quillaja and Yucca additive, from 30 to 49 weeks of age, maintained their productive performance, had improved eggshell and cuticle quality and reduced culled, dirty and contaminated eggs.
Pathology and pathogenesis of cutaneous lesions in beef cattle associated with buffalo fly infestation
Authors: Naseem Muhammad Noman , Allavena Rachel , Raza Ali , Constantinoiu Constantin , McGowan Michael , Turni Conny , Kamran Muhammad , Tabor Ala E. , James Peter
Published: January 2023

Haematobia irritans exigua, commonly known as buffalo fly, is the major hematophagous ectoparasite of north Australian cattle herds. Lesions associated with buffalo fly infestation are generally alopecic, hyperkeratotic, or scab encrusted wounds with variable hemorrhagic ulceration. Buffalo flies can transmit a filarial nematode,Stephanofilariasp., which has been implicated in the pathogenesis of buffalo fly lesions, butStephanofilariainfection has not been detected in all lesions suggesting that other causal factors may be involved. This study characterized the pathology of buffalo fly lesions to identify the role ofStephanofilariain lesion development, as well as to identify other potential agents. Lesion biopsies were collected from north and south Queensland and tested for the presence ofStephanofilariaby qPCR. Each lesion was scored grossly (0–4) for hemorrhage, ulceration, exudation, and alopecia. Lesions were also scored microscopically (0–4) for epidermal and dermal damage and inflammatory characters.Stephanofilariainfection was detected in 31% of lesion biopsies. Grossly,Stephanofilaria-infected lesions had significantly larger lesion area and higher scores for alopecia and hyperkeratosis than lesions where no nematodes were found (P< 0.05). Histologically, epidermal, dermal, and adnexal damage was significantly higher inStephanofilariainfected lesions than lesions without nematodes. Eosinophils, macrophages, and lymphocytes were significantly more abundant inStephanofilariapositive lesions as compared to negative lesions. This study also noted bacterial infection with colonies of coccoid bacteria, observed in skin sections from 19 lesions. Grossly, lesions with bacterial infection had significantly higher ulceration scores compared toStephanofilariapositive lesions, and histologically epidermal disruption was significantly greater in bacteria-infected lesions. We found no evidence of bacteria orStephanofilariainfection in 49% of the lesions assessed and tissue damage patterns and eosinophilic inflammation suggested hypersensitivity to buffalo fly feeding as a possible cause of these lesions. These findings suggest that although the presence ofStephanofilariainfection may increase the severity of lesion pathology, it is not essential for lesion development. These outcomes also suggest a potential role of bacteria and hypersensitivity in pathogenesis of some lesion. A better understanding of buffalo fly lesion etiology will contribute to the optimal treatment and control programmes.
Optimal Design and Testing of a Crawler-Type Flax Combine Harvester
Authors: Shi Ruijie , Dai Fei , Zhao Wuyun , Liu Xiaolong , Wang Tianfu , Zhao Yiming
Published: January 2023

China is a large flax-growing country, with planting area and production ranking among the top three in the world. However, the cultivation range of flax in China is very broad, complex, and diverse, resulting in different planting scales and patterns, making it difficult to apply foreign large combine harvesters, and China lacks a dedicated flax combine harvester. This research improved the design of the 4LZ-4.0 crawler-type flax combine harvester for the regional features and flax cropping patterns in China. First, the structure, technical parameters, and working principles of the machine were introduced; second, the theoretical analysis and optimization of key components were performed; and finally, with the advancing speed of the machine, the speed of the threshing drum, and the speed of the suction fan as independent variables and the rate of removal and the total loss rate as response values, a three-factor, three-level response surface analysis method was used. For each component and response value, a mathematical model was created, and the factors and their interactions were evaluated and confirmed. The results demonstrated that the three parameters impact the threshing drum speed, advancing speed, and centrifugal fan speed in that order of priority, as well as the total loss rate in that order of priority. The machine’s optimal operating settings were 1.5 m·s−1 advancing speed, 788.49 r·min−1 threshing drum speed, and 885.34 r·min−1 centrifugal fan speed, and the validation test results indicated that under the typical dryland dense flax cultivation mode, it had a 97.46% threshing rate and 2.99% total loss rate after the test. This demonstrated that optimizing operational parameters may decrease losses in the process of mechanical flax harvesting, enhance harvesting efficiency, and satisfy the marketable flax harvesting standards.
A Comparative Study of Different Fingertips on the Object Pulling Forces in Robotic Gripper Jaws
Authors: Białek Marcin , Rybarczyk Dominik
Published: January 2023

This paper presents a comparative study of the use of different fingertips in robotic gripper jaws with respect to measuring the pulling force of selected shaped objects from their grasp. The authors built a dedicated test stand and provided methodology to evaluate it. The authors’ innovative approach was to design accessory-controlled jaws for the base of the Robotiq 2F-140 gripper. For the study, rigid structures—flexible soft cushions filled with air and magnetorheological fluid (MRF)—were developed for the jaw. In this way, comparable measurement results were obtained in terms of the structure of the gripper set-up. The secondary purpose of the study was to demonstrate the potential of the soft cushions that are adaptable to the shape of a gripped object. As a result, an adaptive structure was obtained that allows object pulling forces that are comparable to rigid fingertips. In doing so, this does not damage the surface of any of the interacting components. The cushions were made of thermoplastic polyurethane (TPU) formed using 3D printing technology. The results obtained during the implementation of this research may be beneficial for comparing gripper capabilities; thus, they can contribute to advances in smart devices and many industrial fields, including robotics and bioengineering.
Anthropometry, Body Composition, and Physical Fitness in Semi-Professional Soccer Players: Differences between Sexes and Playing Position
Authors: Toro-Román Víctor , Grijota Francisco J. , Muñoz Diego , Maynar-Mariño Marcos , Clemente-Gil Sara , Robles-Gil María C.
Published: January 2023

Performance in soccer has been associated with specific morphological characteristics. Few studies have simultaneously compared the relationships between physical fitness and body composition in both sexes. This study aimed (i) to analyze the differences in anthropometric parameters, body composition, and physical fitness between sexes and playing position in soccer players and (ii) to determine the relationship among anthropometric and body composition parameters to physical fitness parameters according to sex. A total of 50 soccer players (men: n = 26; women: n = 24), participated in the study. Assessments of nutritional intake, anthropometry, body composition, and physical fitness (isometric strength, maximal aerobic capacity, and vertical jump) were carried out. Differences between sexes were observed in all anthropometric and body composition parameters (p < 0.001). There were differences between midfielders and forwards in fat percentage (p < 0.05). Regarding physical condition, differences were found between sexes in all tests performed (p < 0.001). In men soccer players, there were significant correlations between body composition and aerobic capacity (p < 0.05), while in women soccer players, there were correlations with isometric strength tests (p < 0.05). Anthropometry, body composition, and physical fitness differed between sexes. Fat percentages were different among playing positions. There were relationships between anthropometry and body composition with physical fitness. Knowledge of anthropometric and fitness characteristics by playing position and gender in soccer players could help develop specific training programs.
Special Issue on Innovations in the Field of Cloud Computing and Education
Authors: Conde Miguel Á.
Published: January 2023

We are currently in the Digital Society, a continuously evolving context supported by technology [...]
Trust Components: An Analysis in The Development of Type 2 Diabetic Mellitus Mobile Application
Authors: Joshua Salaki Reynaldo , Abbas Wasim , Lee Je-Hoon , Kim Seong Kun
Published: January 2023

Trust in information and communication technology devices is an important factor, considering the role of technology in carrying out supporting tasks in everyday human activities. The level of trust in technology will influence its application and adoption. Recognizing the importance of trust in technology, researchers in this study will examine trust components for the development of a type 2 diabetes mobile application. The results of this study resulted in three major focuses, namely the application design (consisting of architecture), UI design, and evaluation of trust factors of the application: functionality, ease of use, usefulness, security and privacy, and cost. This analysis of trust components will be useful for the application or adoption by users of a type 2 diabetes mellitus mobile application so that users will trust the application both in terms of functionality and the generated information.
Design of an Autonomous Cooperative Drone Swarm for Inspections of Safety Critical Infrastructure
Authors: Jacobsen Rune Hylsberg , Matlekovic Lea , Shi Liping , Malle Nicolaj , Ayoub Naeem , Hageman Kaspar , Hansen Simon , Nyboe Frederik Falk , Ebeid Emad
Published: January 2023

Inspection of critical infrastructure with drones is experiencing an increasing uptake in the industry driven by a demand for reduced cost, time, and risk for inspectors. Early deployments of drone inspection services involve manual drone operations with a pilot and do not obtain the technological benefits concerning autonomy, coordination, and cooperation. In this paper, we study the design needed to handle the complexity of an Unmanned Aerial System (UAS) to support autonomous inspection of safety-critical infrastructure. We apply a constructive research approach to link innovation needs with concepts, designs, and validations that include simulation and demonstration of key design parts. Our design approach addresses the complexity of the UAS and provides a selection of technology components for drone and ground control hardware and software including algorithms for autonomous operation and interaction with cloud services. The paper presents a drone perception system with accelerated onboard computing, communication technologies of the UAS, as well as algorithms for swarm membership, formation flying, object detection, and fault detection with artificial intelligence. We find that the design of a cooperative drone swarm and its integration into a custom-built UAS for infrastructure inspection is highly feasible given the current state of the art in electronic components, software, and communication technology.
Multidirectional Landing Kinetics, Stabilisation Times, and Associated Isokinetic Knee Torques of High-Level Female Netball Players
Authors: Kamffer Lenthea , Hammill Henriette V. , Willemse Yolandi , Kramer Mark
Published: January 2023

Netball is a multidirectional sport afflicted by a relatively high proportion of lower-extremity injuries. The purpose of this study was to evaluate the relationships between multidirectional landing stabilisation times, unilateral landing kinetics, and isokinetic knee joint torques in elite female netball players. A total of 15 players volunteered for the study (age: 20.80 ± 1.42 years; height: 1.75 ± 0.06 m; body mass: 71.69 ± 13.58 kg). All players completed a minimum of 25 multidirectional, unilateral landing tasks, as well as isokinetic dynamometry, to evaluate both concentric and eccentric knee flexion and extension torques. Players showed rapid stabilisation times upon landing (2.02 ± 0.69 s) coupled with moderately high landing forces (4.06 ± 0.82 BWs) and comparatively low isokinetic knee extensor (2.01 ± 0.49 N·m·kg−1) and flexor (1.33 ± 0.30 N·m·kg−1) strength. Moderate positive (r¯ = 0.61) and negative (r¯ = −0.63) correlations were observed between peak isokinetic knee joint strength and the force–time waveform during the early and late weight-acceptance phases of multidirectional jump landings prior to stabilisation. Multidirectional landing kinetics can potentially provide valuable insights related to TTS and possible associations with isolated knee joint musculature during the weight-acceptance phase of jump landings.
Epiphitic Microbiome of Alvarinho Wine Grapes from Different Geographic Regions in Portugal
Authors: Fernandes Paulo , Afonso Isabel Maria , Pereira Jéssica , Rocha Rui , Rodrigues Ana Sofia
Published: January 2023

Geographic location and, particularly, soil and climate exert influence on the typicality of a wine from a specific region, which is often justified by the terroir, and these factors also influence the epiphytic flora associated with the surface of the grape berries. In the present study, the microbiome associated with the surface of berries obtained from ten vineyards of the Alvarinho variety located in different geographical locations in mainland Portugal was determined and analyzed. The removal of microbial flora from the surface of the berries was carried out by washing and sonication, after which the extraction and purification of the respective DNA was carried out. High-throughput short amplicon sequencing of the fungal ITS region and the bacterial 16S region was performed, allowing for the determination of the microbial consortium associated with Alvarinho wine grapes. Analysis of α-diversity demonstrated that parcels from the Monção and Melgaço sub-region present a significantly (p < 0.05) lower fungal diversity and species richness when compared to the plots analyzed from other regions/sub-regions. The ubiquitous presence of Metschnikowia spp., a yeast with enologic potential interest in all parcels from Monção and Melgaço, was also observed.