bluuebunny/crossref_metadata_2025 huggingface dataset:
DOIS PERÍODOS, MESMO PROTAGONISMO?: A INSERÇÃO DA CIDADE DE MOSSORÓ NA AGENDA ESTADUAL DE HABITAÇÃO
Authors: Medeiros Sara Raquel Fernandes Queiroz de , Freitas Mariana Fernandes , Cruz Flávia Duarte de Oliveira
Published: October 2024

Este artigo analisa a produção pública de moradia em Mossoró, Rio Grande do Norte (RN), Brasil, considerando sua inserção na agenda estadual de habitação e seu impacto no território intraurbano, a partir das duas principais políticas habitacionais instituídas no país: a do Banco Nacional da Habitação - BNH (1964-1986) e a do Programa Minha Casa Minha Vida - PMCMV (2009-2020). A pesquisa envolveu uma revisão da literatura com foco na agenda pública de habitação e suas dimensões territoriais, seguindo-se um levantamento sistemático de informações em instituições e seus acervos, tais como o da Companhia de Processamento de Dados do RN; dos jornais e revistas especializadas; do Instituto Brasileiro de Geografia e Estatística; da Companhia Estadual de Habitação e Desenvolvimento Urbano; e o da Caixa Econômica Federal. Contou, ainda, com um trabalho empírico tanto para o mapeamento de uso e ocupação do solo quanto para o registro fotográfico de todos os conjuntos habitacionais empreendidos, em Mossoró, durante o recorte temporal da pesquisa (1964 a 1986 e 2007 a 2020). A análise da política de habitação social no RN, operacionalizada pelas companhias estaduais de habitação, demonstra distintas lógicas de atuação, a depender da política nacional em execução, que impactam diretamente no protagonismo atribuído ao município de Mossoró no cenário da política estadual de habitação. Na escala intraurbana, há semelhanças nas tipologias ofertadas e no modelo de inserção dos empreendimentos no tecido urbano consolidado. O estudo permite inferências para compreender não apenas o caso empírico em foco, mas diferentes municípios brasileiros que executaram projetos das respectivas políticas de habitação.
Sudden Hearing Loss as the Initial Manifestation of Chronic Myeloid Leukemia
Authors: Son Jigon , Chung Da Hyun , SONG JI HYUN , Jung Da Jung
Published: October 2024

Sudden hearing loss rarely presents as an initial manifestation of a hematologic disorder, although it can result from various factors. A 48-year-old male had sudden hearing loss in both ears. The pure tone audiometry and audiometry brainstem response revealed profound hearing loss in both ears. Further examination indicated an elevated leukocyte count, and a temporal bone magnetic resonance image suggested potential bleeding in the cochlear and labyrinth of both ears. Upon consultation with hematologic oncology, chronic myeloid leukemia was diagnosed. Hearing loss in leukemia patients is attributed to leukemic infiltration, hemorrhage, infection, and hyperviscosity. Following treatment using tyrosine kinase inhibitor for chronic myeloid leukemia, the leukocyte count was normalized. However, on the 90th day of treatment, both profound hearing loss persisted. We suspect that the cause of sudden hearing loss may be related to hemorrhage in both cochlear and labyrinth. Accurate diagnosis and treatment are essential for preventing complications.
Pelatihan Pemanfaatan Microsoft PowerPoint dan Doratoon bagi Aparatur Desa Ponggang Kabupaten Subang
Authors: Rahmah Mutia , Hartono Bagus Wahyu , Hamdi Muchlis , Rahman Hardiyanto , Saribulan Nur
Published: October 2024

Sumber daya manusia memainkan peranan penting dalam proses penyelenggaraan pemerintahan negara khususnya pada lingkup desa yang langsung berinteraksi dengan masyarakat. Kedekatan ini tentunya mensyaratkan aparatur desa untuk memiliki keterampilan digital sebagai bentuk komunikasi dengan masyarakat. Keterampilan tersebut dibutuhkan ketika saat sekarang pemerintahan cenderung berkembang ke arah pemerintahan digital dengan Sistem Pemerintahan Berbasis Elektronik (SPBE). Di tengah kenyataan tersebut, desa dan pemerintahan desa masih bekerja dalam pola konvensional, yakni pola pelaksanaan tugas secara manual. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk meningkatkan kompetensi aparatur desa dalam pembuatan bahan presentasi dengan memanfaatkan Microsoft PowerPoint dan Doratoon. Kegiatan pengabdian kepada masyarakat ini dilakukan menggunakan 2 (dua) metode yakni pelatihan dan praktik melalui 4 (empat) tahapan kegiatan mencakup identifikasi, formulasi, implementasi, dan evaluasi kegiatan. Pelaksanaan pelatihan pemanfaatan Microsoft PowerPoint dan Doratoon bagi aparatur Desa Ponggang telah terimplementasikan sesuai dengan tahap identifikasi dan formulasi. Hal ini juga terlihat dari hasil evaluasi yang menunjukkan kepuasan peserta dalam menerima materi pelatihan dan dapat mempraktikkan hasil pelatihan yang telah disampaikan sebelumnya. Ke depan, pelatihan peningkatan kompetensi bagi aparatur Desa Ponggang diharapkan dapat lebih bervariasi dan dilakukan secara berkelanjutan.
Pleurotus ostreatus is a potential biological control agent of root-knot nematodes in eggplant (Solanum melongena)
Authors: Nyangwire Betty , Ocimati Walter , Tazuba Anthony Fredrick , Blomme Guy , Alumai Alfred , Onyilo Francis
Published: October 2024

Introduction

The management of root-knot nematodes has predominantly been based on use of chemicals, which are detrimental to the environment and human health. Biological control provides alternative management. This study evaluated the potential of using Pleurotus ostreatus, an edible mushroom species to control Meloidogyne spp. in eggplants.

Methods

In vitro, the mortality of juveniles (J2) of Meloidogyne spp. were assessed i) P. ostreatus - water suspension with actively growing mycelia, and ii) different dilutions of P. ostreatus PDB broth culture filtrates. In the screen house nematicidal potential of P. ostreatus was tested on eggplants using artificially inoculated soils in a screen house. To attain this, juveniles (J2) of Meloidogyne spp. were inoculated at the base of plants in pots containing P. ostreatus colonized millet grains mixed in 3 kg of soil. The galling index (GI) (scale of 0 to 5), root growth and nematode populations in the different treatments were assessed.

Results and discussion

Mortality of nematodes in the P. ostreatus - water suspension significantly increased with time, reaching over 88% at 48 h and 95% at 72 h. Mortality in undiluted filtrate was consistently significantly higher than the diluted filtrates and control without P. ostreatus. Mortality in the undiluted filtrate increased to 95% at 48 h. When 50 g of P. ostreatus-millet culture was mixed with 3 kg autoclaved pot soil, a GI of 0.95 was observed, dropping to 0.70 when the inoculum was doubled to 100 g. For the treatment without P. ostreatus, a high GI of 2.4 was scored. A significant difference in eggplant root growth and nematode population at (p = 0.02) was found across the treatments. The findings from this study for both in-vitro and pot assay suggest that P. ostreatus and its substrate are potential biological control agents for plant parasitic nematodes in eggplants.
Altered expression of long noncoding RNAs regulating neutrophilic inflammation in peripheral blood was associated with symptom severity in patients with house dust mite-induced allergic rhinitis
Authors: Zhao Jinming , Pu Xiaoyu , Wang Xiangdong , Zhang Luo
Published: October 2024

Background

Long noncoding RNAs (lncRNAs) have been implicated in a diverse array of human immune diseases; however, a comprehensive understanding of the expression and function of lncRNAs in the peripheral blood leukocytes of individuals suffering from house dust mite (HDM)-induced allergic rhinitis (AR) remains elusive.

Objective

To explore the potential roles and functions of long noncoding RNAs (lncRNAs) in the pathogenesis of AR.

Methods

Sequencing analysis was performed on peripheral blood leukocytes collected from patients with HDM-induced AR and healthy controls (HCs) to elucidate the expression patterns of lncRNAs. Differentially expressed (DE) lncRNAs were identified and validated, and further correlation analyses were conducted to explore their associations with visual analog scale (VAS) scores and cytokine levels in the serum and nasal secretions. Additionally, bioinformatics analyses were performed to predict the potential pathways influenced by DE lncRNAs. Finally, the diagnostic potential of these lncRNAs in AR was assessed via receiver operating characteristic (ROC) curve analysis.

Results

Significant differences in the expression profiles of lncRNAs and mRNAs were detected between AR patients and HCs. Four lncRNAs were markedly upregulated in AR patients. AC011524.2 was positively correlated with nasal pruritus (r = 0.4492, P = 0.0411). AL133371.3 was positively correlated with runny nose (r = 0.4889, P = 0.0245). AC011524.2 was positively correlated with CXCL8 (r = 0.4504, P = 0.0035). AL133371.3 was significantly positively correlated with only IL-17 (r = 0.4028, P = 0.0100). IL-4 in the serum was positively related to IL-17 in the serum (r = 0.4163, P = 0.0002). CXCL5 in the serum was positively correlated with IFN-γ (r = 0.3336, P = 0.0354) in nasal secretions. The area under the curve (AUC) of the ROC curve resulting from the integration of the 4 lncRNAs exhibited a remarkable value of 0.940 for AR diagnosis.

Conclusions

Our results identified several lncRNAs associated with AR symptoms and inflammatory cytokines. Specifically, AC011524.2 and AL133371.3 exhibited strong correlations with diverse AR manifestations and serum cytokines, suggesting their pivotal role in the pathogenesis of AR, likely via neutrophil- and Th17-related pathways. However, the precise underlying mechanisms are still elusive, necessitating further exploration.
Radiation protection and natural building materials in cultural heritage
Authors: La Verde Giuseppe , Ricciardelli Alessio , Ognibene Elisa , Ambrosino Fabrizio , Pugliese Mariagabriella
Published: October 2024

Introduction

Buildings that constitute cultural heritage and that are the identity of a defined geographical area are increasingly being restored to offer the community historical places to enjoy. Often the restoration preserves the original structure and building materials, which are usually natural stones. In this study, a radioprotection protocol dedicated to this kind of built environment was proposed and validated.

Methods

After identifying the two predominant types of building material stones (Rosso ammonitico and Pietra Serena), radiometric measurements for natural gamma-emitting radionuclides (Ra-226, Th-232, and K-40) and measurements of the emanation coefficient and calculation of the exhalation rate of radon gas were carried out.

Results

The two types of stone have a content of natural radionuclides that do not exceed the levels recommended by the regulations. The difference between the two types of stone is of an order of magnitude indicating that the red ammonite has a greater radiological impact than the pietra serena.

Discussion

The results, in addition to ensuring the radioprotection of the population, highlighted the need to increase the number of this kind of investigations to implement scientific knowledge and serve the stakeholders involved.
A smarter approach to liquefaction risk: harnessing dynamic cone penetration test data and machine learning for safer infrastructure
Authors: Singh Shubhendu Vikram , Ghani Sufyan
Published: October 2024

This paper presents a novel approach for assessing liquefaction potential by integrating Dynamic Cone Penetration Test (DCPT) data with advanced machine learning (ML) techniques. DCPT offers a cost-effective, rapid, and adaptable method for evaluating soil resistance, making it suitable for liquefaction assessment across diverse soil conditions. This study establishes a threshold criterion based on the ratio of the penetration rate to the dynamic resistance (e/qd), where values exceeding four indicate high liquefaction susceptibility. ML models, including Support Vector Machine (SVM) optimized with Particle Swarm Optimization (PSO), Grey Wolf Optimizer (GWO), Genetic Algorithm (GA), and Firefly Algorithm (FA), were employed to predict the e/qd ratio using key geotechnical parameters, such as fine content, peak ground acceleration, reduction factor, and penetration rate. The SVM-PSO model demonstrated superior performance, with high R2 values of 0.999 and 0.989 in the training and testing phases, respectively. The proposed methodology offers a sustainable and accurate approach for liquefaction assessment, reducing the environmental impact of geotechnical investigations, while ensuring reliable predictions. This study bridges the gap between field testing and advanced computational techniques, providing a powerful tool for geotechnical engineers to assess liquefaction risks and design resilient infrastructures.
Protamine 2 deficiency results in Septin 12 abnormalities
Authors: Sanovec Ondrej , Frolikova Michaela , Kraus Veronika , Vondrakova Jana , Qasemi Maryam , Spevakova Daniela , Simonik Ondrej , Moritz Lindsay , Caswell Drew Lewis , Liska Frantisek , Ded Lukas , Cerny Jiri , Avidor-Reiss Tomer , Hammoud Saher Sue , Schorle Hubert , Postlerova Pavla , Steger Klaus , Komrskova Katerina
Published: October 2024

There is a well-established link between abnormal sperm chromatin states and poor motility, however, how these two processes are interdependent is unknown. Here, we identified a possible mechanistic insight by showing that Protamine 2, a nuclear DNA packaging protein in sperm, directly interacts with cytoskeletal protein Septin 12, which is associated with sperm motility. Septin 12 has several isoforms, and we show, that in the Prm2−/− sperm, the short one (Mw 36 kDa) is mis-localized, while two long isoforms (Mw 40 and 41 kDa) are unexpectedly lost in Prm2−/− sperm chromatin-bound protein fractions. Septin 12 co-immunoprecipitated with Protamine 2 in the testicular cell lysate of WT mice and with Lamin B1/2/3 in co-transfected HEK cells despite we did not observe changes in Lamin B2/B3 proteins or SUN4 expression in Prm2−/− testes. Furthermore, the Prm2−/− sperm have on average a smaller sperm nucleus and aberrant acrosome biogenesis. In humans, patients with low sperm motility (asthenozoospermia) have imbalanced histone–protamine 1/2 ratio, modified levels of cytoskeletal proteins and we detected retained Septin 12 isoforms (Mw 40 and 41 kDa) in the sperm membrane, chromatin-bound and tubulin/mitochondria protein fractions. In conclusion, our findings present potential interaction between Septin 12 and Protamine 2 or Lamin B2/3 and describe a new connection between their expression and localization, contributing likely to low sperm motility and morphological abnormalities.
Characterizing adipocytokine-related signatures for prognosis prediction in prostate cancer
Authors: Fan Shicheng , Liu Haolin , Hou Jian , Zheng Guiying , Gu Peng , Liu Xiaodong
Published: October 2024

Background

Prostate cancer (PCa) is a prevalent malignant tumor in males, with a significant incidence of biochemical recurrence (BCR) despite advancements in treatment. Adipose tissue surrounding the prostate, known as periprostatic adipose tissue (PPAT), contributes to PCa invasion through adipocytokine production. However, the relationship between adipocytokine-related genes and PCa prognosis remains understudied. This study was conducted to provide a theoretical basis and serve as a reference for the use of adipocytokine-related genes as prognostic markers in PCa.

Methods

Transcriptome and survival data of PCa patients from The Cancer Genome Atlas (TCGA) database were analyzed. Differential gene expression analysis was conducted using the DESeq2 and limma packages. Prognostic genes were identified through univariate Cox regression and least absolute shrinkage and selection operator (LASSO) regression. A prognostic model was developed and validated utilizing receiver operating characteristic (ROC) and Kaplan-Meier (K-M) curves. Assessments of immune cell infiltration and drug sensitivity were also carried out. Subsequently, the function of BNIP3L gene in PCa was verified.

Results

A total of 47 adipocytokine-related differentially expressed genes (DEGs) were identified. Five genes (PPARGC1A, APOE, BNIP3L, STEAP4, and C1QTNF3) were selected as prognostic markers. The prognostic model demonstrated significant predictive accuracy in both training and validation cohorts. Patients with higher risk scores exhibited poorer survival outcomes. Immune cell infiltration analysis revealed that the high-risk group had increased immune and ESTIMATE scores, while the low-risk group had higher tumor purity. In vitro experiments confirmed the suppressive effects of BNIP3L on PCa cell proliferation, migration, and invasion.

Conclusion

The prognostic model independently predicts the survival of patients with PCa, aiding in prognostic prediction and therapeutic efficacy. It expands the study of adipocytokine-related genes in PCa, presenting novel targets for treatment.
Identification and characterization of LuxR solo homolog PplR in pathogenic Pseudomonas plecoglossicida NB2011
Authors: Li Shanshan , Jia Tingting , Chi Yu , Chen Jigang , Mao Zhijuan
Published: October 2024

Pseudomonas plecoglossicida is a causative agent of visceral granulomas in large yellow croaker (Larimichthys crocea). Quorum sensing (QS) is widely involved in imparting virulence to pathogenic bacteria; however, it has not been studied in P. plecoglossicida. In this study, we annotated a LuxR family transcriptional regulator in P. plecoglossicida NB2011 and designated as PplR. We aligned the protein sequence by BlastP and Clustal X2, monitored the N-acyl-homoserine lactone (AHL) signal production through cross-feeding bioassay and HC-MS/MS; investigated exogenous AHL signal binding by recombinant expression and thin layer chromatography; constructed a deletion mutant of the target gene by method of double homologous recombination; sequenced the transcript RNA and analyzed the data; additionally, characterized phenotypes of wild type and mutant strain. The LuxR homolog PplR was found to share high similarity with PpoR—the LuxR solo of Pseudomonas putida—without a cognate LuxI. The wild-type strain did not produce any AHL signals and the recombinant LuxR protein was found to bind C6-L-homoserine lactone (C6-HSL), C8-HSL, 3-oxo-C10-HSL, and 3-oxo-C12-HSL. RNA-seq analysis indicated 84 differentially expressed genes—5 upregulated and 79 downregulated—mainly enriched in gene ontology terms, such as flagella-dependent motility, integral component of membrane, DNA binding and transcription, and metal ion binding, suggesting that PplR is a master transcription regulator. The mutant strain showed attenuated biofilm-forming ability and stress resistance, and the data support a role for PplR in the regulation of these traits in P. plecoglossicida NB2011 independent of the presence of AHL signals. This is the first study to provide QS-related information on P. plecoglossicida.
Discovering the effectiveness of climate finance for Somalia’s climate initiatives: a dual-modeling approach with multiple regression and support vector machine
Authors: Nor Mohamed Ibrahim , Mussa Mohamed Barre
Published: October 2024

Introduction

This research investigates into the complex dynamics of climate finance in Somalia, a vulnerable region facing the dire consequences of climate change. The study aims to assess how financial inputs for climate-related projects align with the actual needs and identify critical factors that influence funding effectiveness.

Methods

A dual-methodological approach was employed, integrating both multiple regression analysis and Support Vector Machine (SVM) techniques. This mixed-method analysis facilitates a robust examination of climate finance data to dissect the relationships and impacts of various determinants on funding effectiveness.

Results

The results indicate that adaptation finance, robust governance, and the scale of financial interventions significantly enhance the effectiveness of climate finance flows. However, mitigation finance and aspects related to gender equality displayed less significant impacts. Notably, the study identifies a pervasive underfinancing of climate projects in Somalia, illustrating a significant gap between the needed and actual funds disbursed.

Discussion

The findings underscore the need for enhanced governance frameworks and targeted large-scale financial interventions to optimize the allocation and impact of climate finance in vulnerable regions like Somalia. By quantifying the influence of adaptation finance and governance, this study contributes new insights to the literature on climate finance effectiveness and suggests practical strategies for policymakers and practitioners to improve climate resilience initiatives.
2D speckle tracking echocardiography and comparison with cardiac magnetic resonance in children with acute myocarditis
Authors: Burešová M. , Pavlíček J. , Hanzlíková P. , Tomášková H. , Rybníček O.
Published: October 2024

Background

Cardiac magnetic resonance (CMR) plays a major diagnostic role in acute myocarditis (AM) in children as biopsy is rarely performed in this age group. Contribution of standard echocardiography (ECHO) is limited in AM, but speckle tracking echocardiography (STE) quantitatively characterizes myocardial function, with good sensitivity for detecting subclinical left ventricular (LV) dysfunction and regional kinetics disorders beyond the site of inflammation. This work aimed to evaluate the diagnostic potential of STE as compared with CMR findings in pediatric patients with AM.

Methods

The study was conducted during 2022–2023. Troponin, electrocardiography, ECHO with STE, and CMR with early and late enhancement were performed on each patient. Affected heart segments were analyzed by both STE and CMR, and the correlation of the two methods was assessed.

Results

During the study period, 20 children were diagnosed with AM [14 boys, 6 girls; mean age 12 years (median 14)]. On ECHO, three patients had a deviation in LV biometry, and four patients had a mild systolic function disorder. STE showed at least one affected cardiac segment in all patients, most often the inferolateral segment (16/20; 80%). Of the 20 patients, STE showed a reduction in LV global longitudinal strain in 13 (65%) patients. In all patients, CMR identified an inflammatory focus, most frequently inferolateral (15/20; 75%). The strongest accordance between STE and CMR was observed for the involvement of anterolateral segments (k = 0.88) and the weakest for inferoseptal damage (k = 0.4).

Conclusions

STE can provide important diagnostic information in pediatric patients with AM. This modality supports the detection of early regional edema and subclinical myocardial dysfunction and can determine the impairment severity. STE is non-invasive and repeatable without the need for special patient preparation or for general anesthesia.
Design and implementation of a project management training program to develop workforce ready skills and career readiness in STEM PhD students and postdoctoral trainees
Authors: Salm Elizabeth J. , McKinney Caleb C.
Published: October 2024

A growing number of STEM doctorates pursue careers across the broader biomedical workforce, including industry, policy, and healthcare. Graduate and postdoctoral trainees need training to develop professional skills that prepare them for diverse workforce options. Through engagement with faculty and trainees, we determined that formal management skills are underdeveloped in trainees, particularly around managing projects in a way that is translatable to broader industries. At Georgetown University Medical Center, we adapted Kern's six-steps of curriculum design to develop the Academy for Transferable Management Skills (ATMS) program to help graduate and postdoctoral trainees develop linear experience in utilizing project management tools in their academic research contexts. ATMS includes a self-paced online CANVAS course with learning objectives and content modules that map to the project management cycle from initiation to closure, developed in consultation with PhD-level industry experts. From 2021–2024, 25 trainees have completed the ATMS program, including the capstone project and posttest evaluation. Trainees also complete brief quizzes after each module as a formative assessment of learning. The pre-test evaluation (n = 92) revealed a baseline of project management “pain points” regularly encountered by trainees (risk management, project charters, work breakdown structures, and managing project scope). Posttest data (n = 25) reveal a significant increase (p < 0.0001) in project management self-efficacy measures across the aforementioned pain point scales. Notably, 100% of trainees indicated that they may/would refer the program to colleagues. ATMS offers trainees the flexibility to pick frameworks that apply to their projects with trainees planning to use project schedules (84%), Work Break Down Structures (80%), lessons learned reports (68%), and communication plans (68%) in their work. This integrated experiential learning approach equips trainees to develop and execute their projects according to industry-informed project management principles, which allows them to perform their current research more efficiently and to utilize project management frameworks in a way that is directly transferable to broad careers.
A review of climate change impacts on migration patterns of marine vertebrates in Arctic and Subarctic ecosystems
Authors: Kuletz Katherine J. , Ferguson Steven H. , Frederiksen Morten , Gallagher Colin P. , Hauser Donna D. W. , Hop Haakon , Kovacs Kit M. , Lydersen Christian , Mosbech Anders , Seitz Andrew C.
Published: October 2024

Climate change is impacting marine ecosystems throughout the circumpolar Arctic, altering seasonal habitats and the food bases for fishes, seabirds, and marine mammals. Arctic and Subarctic regions provide resources for resident species and for species that migrate to the north from more southerly regions. Changes in northerly latitudes thus impact endemic as well as non-endemic animals. Herein, we review what is known about climate-driven changes in the migration patterns of Arctic and Subarctic marine vertebrates, including: 1) Arctic residents with seasonal movements – those fishes, seabirds, and marine mammals that complete their entire life cycle within the Arctic but exhibit seasonal movements; 2) Breeding migrants – many seabirds enter the Arctic to breed and subsequently migrate south in the fall; and 3) Summer visitors for feeding – certain species of boreal fishes, seabirds and marine mammals arrive during the northern summer to feed on abundant prey though they breed elsewhere. Migratory movements are often driven by the timing and extent of sea ice, which defines suitable habitat for some animals and limits access to open water and prey for others. Longer open-water seasons, warmer ocean temperatures, and stronger winds have resulted in earlier production blooms in spring and often, extended open-ocean plankton blooms into late summer, resulting in altered prey types and distributions. A common thread among taxa is that shifts in distribution and timing of migrating animals indicate they are traveling farther north, or shifting longitudinally, and migrations are occurring over longer seasonal time frames. Species performing multiple lifetime migrations or long-distance migrants may need to adjust migration timing or routing iteratively to match changes in marine productivity. Altered animal distributions or phenology, and reduced sea ice, affects access to animals that are critical nutritional, economical, and cultural components of Indigenous people’s lives in the Arctic. Ongoing changes challenge the resilience and adaptability of Arctic people and ecosystems, and will require adaptive research and management approaches.
Perceptions of preschoolers and parents on forest pollution
Authors: Demirkaya Hilmi , Köç Ahmet , Sunay Serap , Duygu Hüseyin , Mol Aslı , Günaydın Gülsevil , Günaydın Muhammedi Abdulkerim
Published: October 2024

Introduction

An examination of preschool children’s perceptions and attitudes towards the environment and forests has recently been conducted. However, little research has focused on preschool children’s and their parents’ perceptions of clean and dirty forests. Investigating these perceptions is essential to increase children’s interest in and sensitivity to the environment, thereby fostering their development as responsible individuals who protect the natural world.

Methods

This study aimed to address this knowledge gap by examining the perceptions of clean and dirty forests held by 40 5–6-year-old students and 40 parents, randomly selected from two preschools: one attended by children from high socioeconomic families and the other by children from low socioeconomic families, in a Mediterranean coastal city in southwestern Turkey. A semi-structured questionnaire was used to collect sociodemographic information and perceptions of clean and dirty forests. Additionally, drawing sheets were distributed to the students to illustrate their concepts of clean and dirty forests.

Results

The results indicated that individuals with higher educational levels had more complex and conscious perceptions of clean forests. Children considered abstract concepts, such as supernatural forces and fossil fuels, while parents focused on awareness and conservation status. Conversely, parents and children with lower levels of education tended to concentrate on more concrete and everyday factors, such as litter and natural disasters. Individuals with higher socioeconomic status generally associated forest cleanliness with conscious behaviors and conservation, whereas those with lower socioeconomic status linked it more with polluting factors.

Discussion

This investigation provides valuable insights into the management and control of local forests by incorporating the perspectives of children and their parents, promoting responsible and sustainable tourism practices.
GTasm: a genome assembly method using graph transformers and HiFi reads
Authors: Luo Junwei , Zhang Ziheng , Ma Xinliang , Yan Chaokun , Luo Huimin
Published: October 2024

Motivation

Genome assembly aims to reconstruct the whole chromosome-scale genome sequence. Obtaining accurate and complete chromosome-scale genome sequence serve as an indispensable foundation for downstream genomics analyses. Due to the complex repeat regions contained in genome sequence, the assembly results commonly are fragmented. Long reads with high accuracy rate can greatly enhance the integrity of genome assembly results.

Results

Here we introduce GTasm, an assembly method that uses graph transformer network to find optimal assembly results based on assembly graphs. Based on assembly graph, GTasm first extracts features about vertices and edges. Then, GTasm scores the edges by graph transformer model, and adopt a heuristic algorithm to find optimal paths in the assembly graph, each path corresponding to a contig. The graph transformer model is trained using simulated HiFi reads from CHM13, and GTasm is compared with other assembly methods using real HIFI read set. Through experimental result, GTasm can produce well assembly results, and achieve good performance on NA50 and NGA50 evaluation indicators. Applying deep learning models to genome assembly can improve the continuity and accuracy of assembly results. The code is available from https://github.com/chu-xuezhe/GTasm.
Family planning knowledge, use, and associated factors among women with mental illness and epilepsy in Rwanda: a cross-sectional study
Authors: Mukangabire Pacifique , Umuziga M. Providence , Umubyeyi Benoite , Mukamana Donatilla , Gishoma Darius , Baziga Vedaste , Uwimana Philomene , Sebera Fidele , Bazirete Olive , Kanazayire Clementine
Published: October 2024

Introduction

Family planning knowledge and access to quality family planning services occupy a central position in the lives of all women of reproductive age. However, women with mental illness and epilepsy who are at a high risk of sexual violence, unwanted pregnancies, poor obstetric outcomes, and drug interaction consequences, need it the most. Understanding their family planning knowledge and utilization remains crucial for meeting their needs. The present study aims to assess knowledge, use of family planning, and associated factors among women living with mental illness and epilepsy who attend the Ndera Neuropsychiatric Hospital and affiliated branches.

Methodology

A cross-sectional study was conducted between October 2022 and February 2023. The study involved a purposive sample of 289 women who attended the Ndera Neuropsychiatric Hospital and its two affiliated branches during the data collection period. Psychiatric nurses administered a structured questionnaire. Data were analyzed using descriptive statistics, and multiple logistic regression analysis was performed to assess the factors associated with the use of family planning methods.

Results

Out of 289 who participated in the study, the majority (96.9%) were aware of family planning methods, most (67.8%) had used one method once in their life course, a half (51.9%) were using a family planning method at the time of data collection, and a slight number (26%) expressed intentions of using a family planning method in the future. The most known and used methods were respectively the injectable (17.5%) and oral contraceptive pill (17.5%). Regarding the natural family planning methods, breastfeeding and withdrawal were less used. Being single (AOR = 66.4, 95% CI: 9.8, 44) or married (AOR = 51.4, 95% CI: 11.9–22), having a primary level of education (AOR = 5.6, 95% CI: 2.0, 15.9), knowing a contraceptive method (AOR = 5.8, 95% CI: 0.6, 49) and suffering from brief psychotic disorders (AOR = 2.7, 95% CI: 1.1, 6.6) predicted a higher utilization of family planning.

Conclusion

Most of the women with mental illness in this study were aware of family planning methods and had used one of the family planning methods in life. The national average is below when it comes to family planning awareness. It is important to improve family planning education and counseling for women who attend psychiatric outpatient clinics.
Obstetric violence and associated factors among women who gave birth at public hospitals in Addis Ababa city administration, Ethiopia
Authors: Simeneh Getinet Tilahun , Tesema Getaye Worku , Seifu Befikad Assefa , Tafesse Nebiyou , Hailemariam Abemelek Zegeye , Suleyiman Feruza Mehammed , Nigatu Digafe Tsegaye
Published: October 2024

Background

Obstetric violence during labor and delivery is one of the main reasons that women do not seek care from health caregivers in health facilities. Developing respectful maternity care services for women is the most important approach to ensure better newborn and maternal outcomes.

Objective

This study aimed to assess the magnitude of obstetric violence and associated factors among women who gave birth at public hospitals in Addis Adaba city administration, Ethiopia.

Methods

An institution-based cross-sectional study was carried out among 409 mothers who had given birth at two public hospitals (Gandhi Memorial Hospital and Abebech Gobena Mothers and Children's Health Hospital) in Addis Ababa, Ethiopia, from 1 to 30 May 2023. A systematic sampling method was applied and data were collected using a structured face-to-face interview questionnaire and entered into EpiData 3.1. The data were analyzed using Statistical Package for Social Science version 25. Bi-variable and multivariate analyses were performed. Statistical significance was declared at a P-value <0.05.

Results

In total, 318 mothers [77.8% with a 95% CI (73.64–81.96)] had experienced obstetric violence in the study settings. Being more educated [Adjusted Odds Ratio (AOR) = 6.43; 95% CI 2.92–14.17], having ≥4 antenatal care contacts (AOR = 3.59; 95% CI 1.91–6.75), being multiparous (AOR = 2.65; 95% CI 1.32–5.32), induction of labor (AOR = 3.39; 95% CI 1.69–6.79), vaginal delivery (AOR = 0.25; 95% CI 0.11–0.62), and female birth attendants AOR = 2.42, 95% CI (1.31–4.47) were significantly associated with obstetric violence.

Conclusion

More than three-fourths of the participants experienced obstetric violence. Thus, stakeholders need to develop interventions by taking all risk factors of obstetric violence into account.
Increased thrombin generation in kidney transplant recipients with donor-specific antibodies directed against human leukocyte antigens
Authors: Lóczi Linda , P. Szabó Réka , Orbán-Kálmándi Rita , Hodossy-Takács Rebeka , Szilvási Anikó , Szalai Zoltán , Nagy Gábor , Antal-Szalmás Péter , Nemes Balázs , Bagoly Zsuzsa
Published: October 2024

Introduction

The development of de novo anti-HLA donor specific antibodies (DSAs) is associated with poor outcomes in kidney transplant recipients. It is surmised that an interaction between DSAs and the graft endothelium cause tissue injury, however, the exact underlying pathomechanism and optimal management of patients with DSAs remain undetermined.

Aims

We hypothesized that in kidney transplant recipients the presence of DSAs induce hemostasis alterations, including hypercoagulability, as assessed by the thrombin generation assay (TGA). Patients and methods. In this observational cohort study, 27 kidney transplant recipients with DSAs (DSA+ group) and 16 without DSAs (DSA– group) were enrolled. Venous blood samples were obtained, and besides routine laboratory tests, von Willebrand factor antigen (VWF), FVIII activity, soluble E selectin (sEsel), soluble P selectin (sPsel), TGA, clot lysis assay (CLA), complement levels (C3, C4) were measured. To correlate results with potential changes in DSA status over time, patients were followed and reassessed 6 ± 1.5 months later.

Results

VWF and sPsel did not differ between groups, but both parameters were increased in the majority of patients. Endogenous thrombin potential (ETP) was significantly higher in the DSA+ group as compared to DSA– patients (median:1666; IQR:1438-2012 vs. 1230; IQR:1097-1659 nM*min, p=0.0019). Follow-up measurements indicated that the observed hemostasis alterations were not transient. CLA parameters, C3 and C4 did not differ between DSA+ and DSA– groups. The extent of anti-HLA II DSA positivity correlated positively with ETP, while tacrolimus levels negatively correlated with ETP and VWF/FVIII levels.

Conclusions

In patients with anti-HLA class II DSAs, thrombin generation was significantly increased as compared to DSA– kidney transplant recipients, suggesting that the presence of antibodies is associated with hypercoagulability. Tacrolimus levels were negatively associated with TGA parameters. Hypercoagulability, associated with the presence of DSAs, may potentially contribute to the pathomechanism of antibody-mediated graft injury, warranting future prospective studies.
Advances in the study of artemisinin and its derivatives for the treatment of rheumatic skeletal disorders, autoimmune inflammatory diseases, and autoimmune disorders: a comprehensive review
Authors: Long Zhiyong , Xiang Wang , Xiao Wei , Min Yu , Qu Fei , Zhang Bolin , Zeng Liuting
Published: October 2024

Artemisinin and its derivatives are widely recognized as first-line treatments for malaria worldwide. Recent studies have demonstrated that artemisinin-based antimalarial drugs, such as artesunate, dihydroartemisinin, and artemether, not only possess excellent antimalarial properties but also exhibit antitumor, antifungal, and immunomodulatory effects. Researchers globally have synthesized artemisinin derivatives like SM735, SM905, and SM934, which offer advantages such as low toxicity, high bioavailability, and potential immunosuppressive properties. These compounds induce immunosuppression by inhibiting the activation of pathogenic T cells, suppressing B cell activation and antibody production, and enhancing the differentiation of regulatory T cells. This review summarized the mechanisms by which artemisinin and its analogs modulate excessive inflammation and immune responses in rheumatic and skeletal diseases, autoimmune inflammatory diseases, and autoimmune disorders, through pathways including TNF, Toll-like receptors, IL-6, RANKL, MAPK, PI3K/AKT/mTOR, JAK/STAT, and NRF2/GPX4. Notably, in the context of the NF-κB pathway, artemisinin not only inhibits NF-κB expression by disrupting upstream cascades and/or directly binding to NF-κB but also downregulates multiple downstream genes controlled by NF-κB, including inflammatory chemokines and their receptors. These downstream targets regulate various immune cell functions, apoptosis, proliferation, signal transduction, and antioxidant responses, ultimately intervening in systemic autoimmune diseases and autoimmune responses in organs such as the kidneys, nervous system, skin, liver, and biliary system by modulating immune dysregulation and inflammatory responses. Ongoing multicenter randomized clinical trials are investigating the effects of these compounds on rheumatic, inflammatory, and autoimmune diseases, with the aim of translating promising preclinical data into clinical applications.
Quantification of autoantibodies using a luminescent profiling method in autoimmune interstitial lung disease
Authors: Burbelo Peter D. , Huapaya Julio A. , Khavandgar Zohreh , Beach Margaret , Pinal-Fernandez Iago , Mammen Andrew L. , Chiorini John A. , Noroozi Farhadi Payam , Miller Frederick W. , Schiffenbauer Adam , Sarkar Kakali , Warner Blake M. , Rider Lisa G.
Published: October 2024

Autoantibodies are important for the diagnosis of autoimmune interstitial lung disease (ILD). Standard immunoassays have limitations, including their qualitative nature and/or a narrow dynamic range of detection, hindering the usefulness of autoantibodies as biomarkers of disease activity. Here, the luciferase immunoprecipitation system (LIPS) was evaluated for measuring myositis-specific and other lung-related autoantibodies in 25 subjects with idiopathic inflammatory myopathies (IIM), 26 with Sjögren’s disease (SjD), and 10 healthy volunteers. LIPS detected a broad dynamic range of autoantibodies, to MDA5, Jo-1, PL12, KS, U1-70K, and Ro52, and matched seropositivity status with established immunoassays. Robust anti-MDA5 autoantibodies in four IIM-ILD patients had a median value of 1,134,000 LU (IQR 473,000-2,317,000), which was 500 times higher than in 21 seronegative IIM patients. Markedly elevated anti-Jo-1 autoantibodies in five IIM-ILD patients demonstrated a median value of 1,177,000 LU (IQR: 604,000-2,520,000), which was 1000-fold higher than in seronegative patients. Robust anti-Ro52 and other anti-tRNA-synthetase autoantibodies were detected in a subset of IIM-ILD subjects. In SjD, only anti-U1-70K and KS autoantibodies were identified in ILD patients with a prevalence of 30% and 20%, respectively. In longitudinal samples of five IIM-ILD patients, anti-Jo-1 autoantibody levels paralleled clinical improvement of lung function. LIPS can accurately quantify autoantibody levels as biomarkers for treatment response in patients with autoimmune ILD.
Human genital dendritic cell heterogeneity confers differential rapid response to HIV-1 exposure
Authors: Parthasarathy Siddharth , Moreno de Lara Laura , Carrillo-Salinas Francisco J. , Werner Alexandra , Borchers Anna , Iyer Vidya , Vogell Alison , Fortier Jared M. , Wira Charles R. , Rodriguez-Garcia Marta
Published: October 2024

Dendritic cells (DCs) play critical roles in HIV pathogenesis and require further investigation in the female genital tract, a main portal of entry for HIV infection. Here we characterized genital DC populations at the single cell level and how DC subsets respond to HIV immediately following exposure. We found that the genital CD11c+HLA-DR+ myeloid population contains three DC subsets (CD1c+ DC2s, CD14+ monocyte-derived DCs and CD14+CD1c+ DC3s) and two monocyte/macrophage populations with distinct functional and phenotypic properties during homeostasis. Following HIV exposure, the antiviral response was dominated by DCs’ rapid secretory response, activation of non-classical inflammatory pathways and host restriction factors. Further, we uncovered subset-specific differences in anti-HIV responses. CD14+ DCs were the main population activated by HIV and mediated the secretory antimicrobial response, while CD1c+ DC2s activated inflammasome pathways and IFN responses. Identification of subset-specific responses to HIV immediately after exposure could aid targeted strategies to prevent HIV infection.
A novel functional IKBKE variant activating NFAT in a patient with polyarthritis and a remittent fever
Authors: Yamada Saeko , Nagafuchi Yasuo , Yamada Mamiko , Suzuki Hisato , Natsumoto Bunki , Ota Mineto , Takazawa Ikuo , Hatano Hiroaki , Kono Masanori , Harada Hiroaki , Shoda Hirofumi , Okamura Tomohisa , Kosaki Kenjiro , Fujio Keishi
Published: October 2024

Background

IKBKE is a negative regulator of T cell activation and one of the key activators of type I interferon (IFN) and NFκB signaling via non-classical pathways. The upstream single nucleotide polymorphism of IKBKE (rs2297550-G) is a genome-wide association study risk variant of systemic lupus erythematosus, and is associated with decreased IKBKE expression in T cells by expression quantitative trait locus analysis.

Case presentation

A 48-year-old female had a remittent fever, arthritis, and oral ulcers for 20 years. She had a poor response to corticosteroids or disease-modifying antirheumatic drugs, including the tumor necrosis factor-α antagonist, etanercept, and the anti-interleukin-6 receptor antibody, tocilizumab.

Method

She participated in the Initiative on Rare and Undiagnosed Disease (IRUD), and whole-exome sequencing (WES) was performed. Functional analyses were conducted by transfecting the identified variants into reporter cells to assess the activation of NFAT and NFκB signaling. Additionally, peripheral blood RNA- sequencing (RNA-seq) data were compared with those from healthy individuals to evaluate the gene expression profiles of immune cells.

Result

WES identified a novel heterozygous c.1877G>A, p(Cys626Tyr) variant in IKBKE. Functional analysis indicated that this variant led to increased activity of NFAT (p = 0.015) and decreased activity of NFκB and type I IFN (p = 0.00068 and 0.00044, respectively). The patient had a remarkably low proportion of Naïve CD4 T cells. RNA-seq of peripheral blood immune cell subsets revealed significant differences in gene expression, especially in T cells.

Conclusion

A novel functional heterozygous variant in IKBKE is described in a patient with a remittent fever and arthritis. The data suggest that IKBKE is an important negative regulator of inflammation, particularly in T cells, and this IKBKE variant might be the underlying cause of a novel autoinflammatory pathology.