bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding the Effect of Seasonal Climate Variability on the Salinity in Unsaturated Agricultural Soil
Authors: Wang Hongde , She Dongli , Cardoso Rafaela
Published: November 2023

Salinization/desalinization processes in the soil vadose zone are important to define agricultural irrigation and drainage schedules, especially in reclaimed crop areas. Numerical modeling of soil–climate interaction is a very helpful tool to understand soil salinity distribution and solute transport and therefore define efficient desalination solutions. A finite element analysis program Code_Bright was used to perform a coupled thermo-chemo-hydraulic analysis aiming at investigating the effect of climate actions on the distribution of soil salinity in depth, by modeling solute transport in the vadose zone under fresh/saline groundwater supply. The analysis separated first the effect of rain infiltration and evaporation, and then a real climate was considered as the boundary condition. A downward flow pattern induced by rainfall in the unsaturated zone resulted in a nonlinear salt leaching process. Significant differences in salt concentration between the surface and lower layer caused by rainfall resulted in a decrement in the leaching efficiency. Evaporation causes water to move upward and salt transport to the surface, thus enhancing the soil salinity above the evaporation front. The salinity above the groundwater table and below the evaporation front were less affected regardless of the salinity of the supplied groundwater. The model simulated the salt leaching process during the wet seasons and salt accumulation processes during the dry ones. The soil salinity and saturation at the soil surface have significantly responded to seasonal climate variability. A typical seasonal climate variability would result in a low salt leaching efficiency through years in the coastal reclamation area. These results would be helpful for the design of soil salinization management strategies, such as reducing salt accumulation by reducing evaporation or leaching the surface salt in the dry season, and increasing the drainage to promote leaching in the wet season.
Clustering and Segmentation of Adhesive Pests in Apple Orchards Based on GMM-DC
Authors: Wang Yunfei , Liu Shuangxi , Ren Zhuo , Ma Bo , Mu Junlin , Sun Linlin , Zhang Hongjian , Wang Jinxing
Published: November 2023

The segmentation of individual pests is a prerequisite for pest feature extraction and identification. To address the issue of pest adhesion in the apple orchard pest identification process, this research proposed a pest adhesion image segmentation method based on Gaussian Mixture Model with Density and Curvature Weighting (GMM-DC). First, in the HSV color space, an image was desaturated by adjusting the hue and inverting to mitigate threshold crossing points. Subsequently, threshold segmentation and contour selection methods were used to separate the image background. Next, a shape factor was introduced to determine the regions and quantities of adhering pests, thereby determining the number of model clustering clusters. Then, point cloud reconstruction was performed based on the color and spatial distribution features of the pests. To construct the GMM-DC segmentation model, a spatial density (SD) and spatial curvature (SC) information function were designed and embedded in the GMM. Finally, experimental analysis was conducted on the collected apple orchard pest images. The results showed that GMM-DC achieved an average accurate segmentation rate of 95.75%, an average over-segmentation rate of 2.83%, and an average under-segmentation rate of 1.42%. These results significantly outperformed traditional image segmentation methods. In addition, the original and improved Mask R-CNN models were used as recognition models, and the mean Average Precision was used as the evaluation metric. Recognition experiments were conducted on pest images with and without the proposed method. The results show the mean Average Precision for pest images segmented with the proposed method as 92.43% and 96.75%. This indicates an improvement of 13.01% and 12.18% in average recognition accuracy, respectively. The experimental results demonstrate that this method provides a theoretical and methodological foundation for accurate pest identification in orchards.
Genome-Wide Identification, Characterization, and Expression Analysis under Abiotic Stresses of the UBP Gene Family in Rice (Oryza sativa L.)
Authors: Zou Xiaoxiao , Li Yongliang , Yin Huangping , Xu Jiajin , Li Zeqi , Jiang Shuai , Chen Fenglin , Li You , Xiao Wenjun , Liu Shucan , Guo Xinhong
Published: November 2023

The ubiquitin-specific protease (UBP) family represents a type of deubiquitinase (DUB) that plays a significant role in plant abiotic stress responses, growth, and development. Although UBP genes have been characterized in many species, a genome-wide systematic analysis and the potential functions under abiotic stresses have not yet been reported in rice (Oryza sativa L.). In this study, we identified 21 UBP gene family members in the genome of rice, all of which had UCH domains containing short but highly conserved Cys-box and His-box. The DUF4220 and DUF594 domains, which are found in monocotyledonous plants, were unique. Phylogenetic analysis suggested that OsUBP7 and OsUBP12 genes had the closest genetic relationships with AtUBP12 and AtUBP13, implying that OsUBP7 and OsUBP12 might have similar biological functions to AtUBP12 and AtUBP13. Synteny analysis revealed that OsUBP genes were expanded through segmental duplication under purifying selection. Meanwhile, they had closer evolutionary relationships with monocotyledonous plants. Additionally, all OsUBP genes had an ABRE cis-element, which was related to abscisic acid (ABA) stress hormone response. Protein interaction analysis suggested that OsUBP22 might modulate gene regulation through OsGCN5- and OsADA2-mediated chromatin modification, and OsUBP10 might regulate salt stress through modulating OsDNA2_4 stability. Specifically, the expression profiles and the transcriptional levels of 11 representative OsUBPs suggested the potential important functions of OsUBP9 and OsUBP17 under drought and cold stresses. Taken together, our study provided a systematic analysis of OsUBPs and preliminarily explored their potential roles under abiotic stresses. Our results lay a foundation for further research on the functions of the OsUBP gene family under abiotic stresses.
Evaluation of Variable Application Rate of Fertilizers Based on Site-Specific Management Zones for Winter Wheat in Small-Scale Farming
Authors: Wang Yuefan , Yuan Yifan , Yuan Fei , Ata-UI-Karim Syed Tahir , Liu Xiaojun , Tian Yongchao , Zhu Yan , Cao Weixing , Cao Qiang
Published: November 2023

China is currently experiencing a severe issue of excessive fertilization. Variable rate fertilization (VRF) technology is key to solving this issue in precision agriculture, and one way to implement VRF is through management zone (MZ) delineation. This study is aimed at evaluating the feasibility and potential benefits of VRF based on site-specific MZs in smallholder farm fields. This study determined the amounts of basal and top-dressing fertilizers in different spatial units, based on soil nutrient MZs and crop growth MZs, respectively. The potential agronomic, economic, and environmental advantages of spatial variable rate fertilization were further assessed by comparing the farmer’s treatment, the expert’s treatment, and the variable rate fertilization treatment based on management zones (VR-MZ). The results showed that VR-MZ reduced the use of nitrogen (N), phosphorus (P), and potassium (K) fertilizers by 22.90–43.95%, 59.11–100%, and 8.21–100%, respectively, and it also increased the use efficiency of N, P, and K by 12.27–28.71, 89.64–176.85, and 5.48–266.89 kg/kg, respectively, without yield loss. The net incomes of VR-MZ were 15.5–449.61 USD ha−1 higher than that of traditional spatially uniform rate fertilization. Meanwhile, less nitrous oxide emission (23.50–45.81%), ammonia volatilization (19.38–51.60%), and nitrate ion leaching amounts (28.77–53.98%) were found in VR-MZ compared to those in uniform fertilization. The results suggest that the VR-MZ has great potential for saving fertilizers, significantly increasing farmers’ net income, reducing environmental pollution, and promoting the sustainable use of resources. This study provides a theoretical basis and technical support for exploring a VRF suitable for village-scale farming.
Evaluation of Foot and Claw Lesions and Claw Horn Growth in Piglets from Birth to End of Nursery
Authors: Bernau Maren , Meckel Hannah , Dölle Theresa , Scholz Armin Manfred
Published: November 2023

The objective of this study is to evaluate foot lesions and claw horn growth of piglets from the day of birth until the end of the suckling period and describe the prevalence and extent in three different genetic lines. Therefore, bruising, dorsal horn lesions, claw horn growth, and weight gain were evaluated five times during growth, starting with the day of birth (day 0 of life) and ending with the end of nursery (day 68 ± 2 of life). Totally, 74 piglets of three genetic lines (German Landrace × Large White; Piétrain × Piétrain-Duroc; pure German Landrace) were examined. Bruising and dorsal horn lesions reached maximum levels at day 7 (±1) of life (with up to 91% of piglets having bruising marks and up to 94.1% of piglets having dorsal horn lesions). Differences among genetic lines were detected, with German Landrace × Large White crossbreds showing the highest percentage of bruising, but Piétrain × Piétrain-Duroc crossbreds showed the highest score for dorsal horn lesions at day 0. Until weaning (day 28 ± 1), front feet were more affected by bruising than hind feet (70.3% of the front feet and 64% of the hind feet showed bruising), but at the end of nursery (day 68 ± 2), hind feet showed a higher percentage of affected feet than front feet (65.5% vs. 41.3%). Several factors affect bruising scores in piglets, including body weight, age at examination, litter size, sex, parity, breed, and claw horn length. Additionally, significant differences for claw horn length were detected among the genetic lines from birth to end of nursery.
Vertebral Osteosarcoma in Two Cats—Diagnosis, Treatment, and Outcome
Authors: Santifort Koen Maurits , Beukers Martijn , Roos Arno , van Rijswoud Benjamin , Meertens Nadine , Peperkamp Klaas , Ben-Amotz Ron , Bergknut Niklas
Published: November 2023

In this case report, we describe the diagnosis, treatment, and outcome of two feline cases of vertebral osteosarcoma. Case 1: A 6-year-old female neutered domestic longhaired cat was presented with progressive paraparesis, ataxia, and spinal hyperesthesia. MRI of the thoracolumbar spinal cord and vertebral column revealed a strongly contrast-enhancing mass lesion originating from the dorsal lamina and spinous process of T13. The lesion caused extradural compression of the spinal cord. Surgical debulking was performed, and the histopathological evaluation of surgical biopsies was consistent with vertebral osteosarcoma. The cat was paraplegic with intact nociception post-surgery. Subsequently, the cat recovered ambulation while remaining mildly ataxic and paraparetic at long-term follow-up. Post-operative chemotherapy was started with doxorubicin. CT scans at 2, 4, 9, 13, and 20 months post-surgery showed no signs of local recurrence or metastasis. Case 2: A 15.5-year-old male neutered domestic shorthaired cat was presented with progressive paraparesis, tail paresis, and spinal hyperesthesia. Radiographs and CT scan of the lumbar vertebral column showed a large mass originating from the dorsal lamina and spinous process of L6, suggestive of neoplasia, with severe compression of the spinal cord. Surgical debulking was performed, and the histopathological evaluation was consistent with vertebral osteosarcoma. Post-operative chemotherapy was started with doxorubicin. Seven months post-surgery, the patient was neurologically normal with no signs of metastatic disease. This case report highlights the possibility of good outcomes after the surgical treatment of feline vertebral osteosarcoma supplemented with post-surgical chemotherapy.
Histological Characteristics of Conjunctiva-Associated Lymphoid Tissue in Young and Adult Holstein Cattle
Authors: Kosenda Keigo , Ichii Osamu , Yamashita Yusuke , Ohtsuka Hiromichi , Fukuda Shigeo , Kon Yasuhiro
Published: November 2023

The conjunctiva-associated lymphoid tissue (CALT) has been used as a target site for mucosal vaccinations in several animals. In this study, we compared the morphological features of CALT in the eyelid and third eyelid between Holstein calves and adult cows. In the eyelids, CALTs in the form of diffused lymphoid tissue (DLT) and lymphatic follicles (LF) were observed, where DLTs were dominant and LFs were scarce. The CALTs of cows comprised T-, B-cells, macrophages, and antigen-presenting cells (APCs). In particular, B-cells were dominant except in the eyelids of the calves. The epithelial layer covering the CALT is often discontinuous and lacks goblet cells. Cytokeratin18 is strongly expressed in the epithelial layer covering the CALT, except in the third eyelids of adult cows. IgA-positive cells were diffusely distributed in the lamina propria of the conjunctiva of the eyelids and third eyelids. The eyelid CALT area in calves was lower than that in adult cows. Furthermore, the CALT of calves had a lower cellularity of B-cells and a higher cellularity of macrophages than that of adult cows. These histological characteristics indicate that CALT plays a role in the mucosal immune-inductive and effector sites. Furthermore, lower cellularity of B-cells in the CALT of calves indicates that the function of CALT as a mucosal immune induction site is less developed in calves than in adult cows.
The Expression of Selected Cytokine Genes in the Livers of Young Castrated Bucks after Supplementation with a Mixture of Dry Curcuma longa and Rosmarinus officinalis Extracts
Authors: Urbańska Daria Maria , Pawlik Marek , Korwin-Kossakowska Agnieszka , Rutkowska Karolina , Kawecka-Grochocka Ewelina , Czopowicz Michał , Mickiewicz Marcin , Kaba Jarosław , Bagnicka Emilia
Published: November 2023

The study aims to determine the effect of supplementation with a mixture of Curcuma longa and Rosmarinus officinalis extracts (896:19 ratio) on the expression of 15 cytokine genes in the livers of 20 castrated goat bucks. Two equal groups were created: treated and control groups. The treated group was provided a mixture (1.6 g/day/buck) for 124 days. Liver tissue samples were collected after slaughter. The gene expression was analyzed using RT-qPCR with two reference genes. Variance analysis was conducted using a model with the group fixed effect. IL-2 and IL-8 expression was below the detection level. No differences were found for IL-1α, IL-1β, IL-4, IL-6, IL-10, IL-16, IFN-α, IFN-β, TNF-α, and CCL4 expressions, suggesting that supplementation does not activate cytokine production in the healthy hepatocytes. The treated group demonstrated lower IL-12 expression (p < 0.05) and a tendency for higher IL-18 and INF-γ (0.05 < p < 0.10) expressions, which may indicate a hypersensitivity resulting from excessive supplement dose. The increased IFN-γ expression could be caused by the increased IL-18 expression. If a small dose of extract can induce an allergic reaction in young goat bucks, it is also possible that humans may be susceptible to an overdose of curcumin and/or turmeric extracts.
Impacts of Different Processes on the Nutritional and Antinutritional Contents of White and Blue Lupin Seeds and Usage Possibilities for Sustainable Poultry Production
Authors: Uzun Tugce , Agma Okur Aylin
Published: November 2023

In the current era, it is important to consider economic and ecological sustainability issues while optimally meeting the nutrient needs of poultry. The use and research of alternative feedstuffs have gained importance due to these factors. The aim of this study is to reveal the raw lupin seeds’ nutrient ingredients as an alternative feedstuff and the effects of debittering methods. In the present study, two different treatments (germination for 2 days; heat treatment in an autoclave at 130 °C for 20 min) were applied to white and blue lupin seeds, and the differences in nutrient compositions between them and raw seeds were determined. When fatty acid compositions were analyzed, oleic, γ-linolenic, arachidic, behenic, erucic, and lignoceric acid values were found to be the highest in the raw, autoclaved, and germinated forms of white lupin (p < 0.01). The highest values of palmitic, stearic, and linoleic acids were observed in blue lupin (p < 0.01). While the value of total quinolizidine alkaloids (QA) in raw white lupin grains was higher than 1.943 mg/g, it was higher than 1.800 mg/g in autoclaved and germination-treated grains. Similarly, the total QA value of raw blue lupin grains was 0.894 mg/g, 0.609 ± 0.244 mg/g in germination-treated seeds, and 0.705 ± 0.282 mg/g in autoclave-treated seeds. As a result of these findings, it can be said that the methods applied for the removal of bitterness gave promising results. Furthermore, it would be rewarding to use these lupin varieties in in vitro and in vivo experiments to reveal the impacts and mechanisms of debittering methods on poultry.
Urinary Tract Infections in Men in Primary Care in Catalonia, Spain
Authors: Fernández-García Silvia , Moragas Moreno Ana , Giner-Soriano Maria , Morros Rosa , Ouchi Dan , García-Sangenís Ana , Monteagudo Mònica , Monfà Ramon , Llor Carl
Published: November 2023

Antimicrobial resistance is a major global problem that is primarily driven by the excessive and inappropriate utilization of antibiotics. Urinary tract infections (UTIs) are frequent in primary health care (PHC) and are typically treated with antibiotics. There is ample evidence on the management of this condition in women but not in men. The aim of this study was to describe the epidemiology of UTIs in men in Catalonia, Spain. We conducted a population-based observational cohort study that included male patients diagnosed with UTI within our SIDIAP and CMBD database during the period from 2012 to 2021. UTI diagnoses were grouped into five main groups (cystitis, prostatitis, orchitis and epididymitis, urethritis, and pyelonephritis). Of the 316,762 men with at least one recorded UTI episode, the majority were registered with a diagnosis of cystitis in PHC (212,958 patients). Quinolones were the most commonly recorded treatment for UTIs (between 18.3% and 38.6%, depending on the group), except for urethritis in which a combination of antibiotics (36.7%) was most frequently used. The treatment duration period was between 9 days and 18 days, except for the prostatitis group, in which treatment was extended to 21 days. Urine cultures were documented in up to 30% in the cystitis group. Pyelonephritis was the category linked to most septicemia cases (3.0%). Conclusions: This is the first study to assess UTIs in men using a large PHC database in Spain. The sociodemographic characteristics of our sample are similar to other studies in the literature. In our setting, the use of quinolones for the treatment of UTIs is the most registered, and its duration was between 9 days and 18 days, despite the fact that resistance to quinolones exceeds 20% of the strains in our area.
Meropenem/Vaborbactam: β-Lactam/β-Lactamase Inhibitor Combination, the Future in Eradicating Multidrug Resistance
Authors: Duda-Madej Anna , Viscardi Szymon , Topola Ewa
Published: November 2023

Due to the fact that there is a steadily increasing trend in the area of antimicrobial resistance in microorganisms, there is a need to look for new treatment alternatives. One of them is the search for new β-lactamase inhibitors and combining them with β-lactam antibiotics, with the aim of increasing the low-dose efficacy, as well as lowering the resistance potential of bacterial strains. This review presents the positive effect of meropenem in combination with a vaborbactam (MER-VAB). This latest antibiotic-inhibitor combination has found particular use in the treatment of infections with the etiology of carbapenem-resistant Enterobacterales (CRE), Gram-negative bacteria, with a high degree of resistance to available antimicrobial drugs.
Distribution of Extended-Spectrum-β-Lactamase-Producing Diarrheagenic Escherichia coli Clonal Complex 10 Isolates from Patients with Diarrhea in the Republic of Korea
Authors: Park Jungsun , Shin Eunkyung , Han Joohyun , Kang Wooju , Yoo Jaeil , Yoo Jung-Sik , Roh Dong-Hyun , Kim Junyoung
Published: November 2023

ESBL-producing E. coli is a public health concern in healthcare settings and the community. Between 2009 and 2018, a total of 187 ESBL-producing pathogenic E. coli isolates were identified, and clonal complex (CC) 10 was the predominant clone (n = 57). This study aimed to characterize the ESBL-producing pathogenic E. coli CC10 strains obtained from patients with diarrhea to improve our understanding of CC10 distribution in the Republic of Korea. A total of 57 CC10 strains were selected for comprehensive molecular characterization, including serotype identification, the analysis of antibiotic resistance genes, the investigation of genetic environments, the determination of plasmid profiles, and the assessment of genetic correlations among CC10 strains. Among the CC10 isolates, the most prevalent serotype was O25:H16 (n = 21, 38.9%), followed by O6:H16 (10, 19.6%). The most dominant ESBL genes were blaCTX-M-15 (n = 31, 55%) and blaCTX-M-14 (n = 15, 27%). Most blaCTXM genes (n = 45, 82.5%) were located on plasmids, and these incompatibility groups were confirmed as IncB/O/K/Z, IncF, IncI1, and IncX1. The mobile elements located upstream and downstream mainly included ISEcp1 (complete or incomplete) and IS903 or orf477. Phylogenetic analysis showed that the CC10 strains were genetically diverse and spread among several distinct lineages. The results of this study show that ESBL-producing pathogenic E. coli CC10 has been consistently isolated, with CTX-M-15-producing E. coli O25:H16 isolates being the major type associated with the distribution of CC10 clones over the past decade. The identification of ESBL-producing pathogenic E. coli CC10 isolates underscores the possible emergence of resistant isolates with epidemic potential within this CC. As a result, continuous monitoring is essential to prevent the further dissemination of resistant ESBL-producing E. coli CC10 strains.
Antimicrobial Resistance in Commensal Escherichia coli of the Porcine Gastrointestinal Tract
Authors: O’Neill Lorcan , Manzanilla Edgar García , Ekhlas Daniel , Leonard Finola C.
Published: November 2023

Antimicrobial resistance (AMR) in Escherichia coli of animal origin presents a threat to human health. Although animals are not the primary source of human infections, humans may be exposed to AMR E. coli of animal origin and their AMR genes through the food chain, direct contact with animals, and via the environment. For this reason, AMR in E. coli from food producing animals is included in most national and international AMR monitoring programmes and is the subject of a large body of research. As pig farming is one of the largest livestock sectors and the one with the highest antimicrobial use, there is considerable interest in the epidemiology of AMR in E. coli of porcine origin. This literature review presents an overview and appraisal of current knowledge of AMR in commensal E. coli of the porcine gastrointestinal tract with a focus on its evolution during the pig lifecycle and the relationship with antimicrobial use. It also presents an overview of the epidemiology of resistance to extended spectrum cephalosporins, fluoroquinolones, and colistin in pig production. The review highlights the widespread nature of AMR in the porcine commensal E. coli population, especially to the most-used classes in pig farming and discusses the complex interplay between age and antimicrobial use during the pig lifecycle.
Role of Relebactam in the Antibiotic Resistance Acquisition in Pseudomonas aeruginosa: In Vitro Study
Authors: Ventero Maria Paz , Haro-Moreno Jose M. , Molina-Pardines Carmen , Sánchez-Bautista Antonia , García-Rivera Celia , Boix Vicente , Merino Esperanza , López-Pérez Mario , Rodríguez Juan Carlos
Published: November 2023

Background: Pseudomonas aeruginosa shows resistance to several antibiotics and often develops such resistance during patient treatment. Objective: Develop an in vitro model, using clinical isolates of P. aeruginosa, to compare the ability of the imipenem and imipenem/relebactam to generate resistant mutants to imipenem and to other antibiotics. Perform a genotypic analysis to detect how the selective pressure changes their genomes. Methods: The antibiotics resistance was studied by microdilution assays and e-test, and the genotypic study was performed by NGS. Results: The isolates acquired resistance to imipenem in an average of 6 days, and to imipenem/relebactam in 12 days (p value = 0.004). After 30 days of exposure, 75% of the isolates reached a MIC > 64 mg/L for imipenem and 37.5% for imipenem/relebactam (p value = 0.077). The 37.5% and the 12.5% imipenem/relebactam mutants developed resistance to piperacillin/tazobactam and ceftazidime, respectively, while the 87.5% and 37.5% of the imipenem mutants showed resistance to these drugs (p value = 0.003, p value = 0.015). The main biological processes altered by the SNPs were the glycosylation pathway, transcriptional regulation, histidine kinase response, porins, and efflux pumps. Discussion: The addition of relebactam delays the generation of resistance to imipenem and limits the cross-resistance to other beta-lactams. The clinical relevance of this phenomenon, which has the limitation that it has been performed in vitro, should be evaluated by stewardship programs in clinical practice, as it could be useful in controlling multi-drug resistance in P. aeruginosa.
Antibiofilm Effect of Nitric Acid-Functionalized Carbon Nanotube-Based Surfaces against E. coli and S. aureus
Authors: Gomes Marisa , Teixeira-Santos Rita , Gomes Luciana C. , Sousa-Cardoso Francisca , Carvalho Fábio M. , Tomé Andreia R. , Soares Olívia S. G. P. , Whitehead Kathryn A. , Mergulhão Filipe J.
Published: November 2023

Chemically modified carbon nanotubes are recognized as effective materials for tackling bacterial infections. In this study, pristine multi-walled carbon nanotubes (p-MWCNTs) were functionalized with nitric acid (f-MWCNTs), followed by thermal treatment at 600 °C, and incorporated into a poly(dimethylsiloxane) (PDMS) matrix. The materials’ textural properties were evaluated, and the roughness and morphology of MWCNT/PDMS composites were assessed using optical profilometry and scanning electron microscopy, respectively. The antibiofilm activity of MWCNT/PDMS surfaces was determined by quantifying culturable Escherichia coli and Staphylococcus aureus after 24 h of biofilm formation. Additionally, the antibacterial mechanisms of MWCNT materials were identified by flow cytometry, and the cytotoxicity of MWCNT/PDMS composites was tested against human kidney (HK-2) cells. The results revealed that the antimicrobial activity of MWCNTs incorporated into a PDMS matrix can be efficiently tailored through nitric acid functionalization, and it can be increased by up to 49% in the absence of surface carboxylic groups in f-MWCNT samples heated at 600 °C and the presence of redox activity of carbonyl groups. MWCNT materials changed the membrane permeability of both Gram-negative and Gram-positive bacteria, while they only induced the production of ROS in Gram-positive bacteria. Furthermore, the synthesized composites did not impact HK-2 cell viability, confirming the biocompatibility of MWCNT composites.
Assessing the Potential of Gallic Acid and Methyl Gallate to Enhance the Efficacy of β-Lactam Antibiotics against Methicillin-Resistant Staphylococcus aureus by Targeting β-Lactamase: In Silico and In Vitro Studies
Authors: Jiamboonsri Pimsumon , Eurtivong Chatchakorn , Wanwong Sompit
Published: November 2023

Methicillin-resistant Staphylococcus aureus (MRSA), a global health concern, has prompted research into antibiotic adjuvants as a potential solution. Although our group previously reported the enhancing effects of gallic acid (GA) and methyl gallate (MG) on penicillin G activity against MRSA, the synergistic potential with other β-lactam antibiotics and the underlying mechanism have not been fully explored. Therefore, this study primarily aimed to investigate the antibacterial synergism with β-lactam antibiotics through disc diffusion, checkerboard, and time–kill assays. The β-lactamase inhibition was also examined through both molecular modeling and in vitro experiments. Additionally, bacterial morphology changes were studied using a scanning electron microscopy (SEM). The results revealed that both GA and MG exhibited anti-MRSA activity and showed indifferent effects when combined with β-lactam antibiotics against methicillin susceptible S. aureus (MSSA). Interestingly, MG demonstrated synergism with only the β-lactamase-unstable antibiotics against MRSA with the lowest fractional inhibitory concentration (FIC) indexes of ≤3.75. However, GA and MG exhibited weak β-lactamase inhibition. Furthermore, GA, MG, and the combination with ampicillin induced the morphological changes in MRSA, suggesting a possible mechanism affecting the cell membrane. These findings suggest that MG could potentially serve as an adjunct to β-lactam antibiotics to combat MRSA infections.
Heme Oxygenase-1 and Its Role in Colorectal Cancer
Authors: Fahrer Jörg , Wittmann Simon , Wolf Ann-Cathrin , Kostka Tina
Published: November 2023

Heme oxygenase-1 (HO-1) is an enzyme located at the endoplasmic reticulum, which is responsible for the degradation of cellular heme into ferrous iron, carbon monoxide and biliverdin-IXa. In addition to this main function, the enzyme is involved in many other homeostatic, toxic and cancer-related mechanisms. In this review, we first summarize the importance of HO-1 in physiology and pathophysiology with a focus on the digestive system. We then detail its structure and function, followed by a section on the regulatory mechanisms that control HO-1 expression and activity. Moreover, HO-2 as important further HO isoform is discussed, highlighting the similarities and differences with regard to HO-1. Subsequently, we describe the direct and indirect cytoprotective functions of HO-1 and its breakdown products carbon monoxide and biliverdin-IXa, but also highlight possible pro-inflammatory effects. Finally, we address the role of HO-1 in cancer with a particular focus on colorectal cancer. Here, relevant pathways and mechanisms are presented, through which HO-1 impacts tumor induction and tumor progression. These include oxidative stress and DNA damage, ferroptosis, cell cycle progression and apoptosis as well as migration, proliferation, and epithelial-mesenchymal transition.
Sarcopenia Is Associated with Changes in Circulating Markers of Antioxidant/Oxidant Balance and Innate Immune Response
Authors: Bellanti Francesco , Lo Buglio Aurelio , Quiete Stefano , Dobrakowski Michał , Kasperczyk Aleksandra , Kasperczyk Sławomir , Vendemiale Gianluigi
Published: November 2023

(1) Background: The involvement of redox balance alterations and innate immunity is suggested to play a key role in the pathogenesis of sarcopenia. This investigation aimed to define and relate modifications in circulating markers of redox homeostasis and the innate immune response in human sarcopenia. (2) Methods: A total of 32 subjects aged >65 years old and affected by sarcopenia according to the second “European Working Group on sarcopenia in older people” guidelines were compared with 40 non-sarcopenic age-matched controls. To assess systemic redox homeostasis, reduced (GSH) and oxidized (GSSG) blood glutathione and plasma malondialdehyde (MDA)– and 4-hydroxy-2,3-nonenal (HNE)–protein adducts were measured. Immune cells and circulating interleukins were determined to compare the innate immune response between both groups. (3) Results: Impaired redox balance in sarcopenic patients, characterized by a high blood GSSG/GSH ratio and plasma MDA/HNE–protein adducts, was sustained by reduced antioxidants in peripheral blood mononuclear cells. Furthermore, sarcopenic patients showed higher neutrophil-to-lymphocyte ratios and interleukin (IL)-4, IL-6, IL-10, and tumor necrosis factor (TNF) with respect to non-sarcopenic patients. Linear regression analysis resulted in a strong association between redox balance and immune response markers in the sarcopenic group. (4) Conclusions: These results support the interplay between redox homeostasis alteration and disruption of the innate immune response in the pathogenesis of sarcopenia.
Antioxidant Activity of an Aqueous Extract of Cuttlefish Ink during Fish Muscle Heating
Authors: Trigo Marcos , Paz David , Bote Antía , Aubourg Santiago P.
Published: November 2023

The antioxidant effect of cuttlefish (Sepia officinalis) ink (CFI) was analysed in the present study. A model system consisting of minced seabream (Sparus aurata) muscle and different concentrations of an aqueous extract of CFI was subjected to a heat (50 °C) treatment for 12 days. The effects of the CFI content and the heating time on lipid oxidation (conjugated diene (CD), conjugated triene (CT), and peroxide values and fluorescent compound formation), hydrolysis (free fatty acid content) development, and changes in the fatty acid (FA) profile (polyene index (PI), unsaturated FA content, ω3/ω6 ratio) were determined. The addition of the aqueous extract of CFI led to a lower (p < 0.05) development of lipid oxidation (CD, CT, and fluorescent compound determination) and to a higher (p < 0.05) retention of unsaturated FAs (PI determination). More important effects were found with increased CFI concentrations and at advanced heating times. However, a definite effect on lipid hydrolysis development (FFA value) could not be inferred. A new approach for the beneficial use of cuttlefish ink is presented. According to the direct relationship between rancidity stability and nutritional and sensory values, the present study provides a new strategy for the quality enhancement of thermally treated seafood.
Brain Iron Homeostasis and Mental Disorders
Authors: Wu Qiong , Ren Qiuyang , Meng Jingsi , Gao Wei-Juan , Chang Yan-Zhong
Published: November 2023

Iron plays an essential role in various physiological processes. A disruption in iron homeostasis can lead to severe consequences, including impaired neurodevelopment, neurodegenerative disorders, stroke, and cancer. Interestingly, the link between mental health disorders and iron homeostasis has not received significant attention. Therefore, our understanding of iron metabolism in the context of psychological diseases is incomplete. In this review, we aim to discuss the pathologies and potential mechanisms that relate to iron homeostasis in associated mental disorders. We propose the hypothesis that maintaining brain iron homeostasis can support neuronal physiological functions by impacting key enzymatic activities during neurotransmission, redox balance, and myelination. In conclusion, our review highlights the importance of investigating the relationship between trace element nutrition and the pathological process of mental disorders, focusing on iron. This nutritional perspective can offer valuable insights for the clinical treatment of mental disorders.
Saturation-Based Airlight Color Restoration of Hazy Images
Authors: Chung Young-Su , Kim Nam-Ho
Published: November 2023

Typically, images captured in adverse weather conditions such as haze or smog exhibit light gray or white color on screen; therefore, existing hazy image restoration studies have performed dehazing under the same assumption. However, hazy images captured under actual weather conditions tend to change color because of various environmental factors such as dust, chemical substances, sea, and lighting. Color-shifted hazy images have hindered accurate color perception of the images, and due to the dark haze color, they have worsened visibility compared to conventional hazy images. Therefore, various color correction-based dehazing algorithms have recently been implemented to restore colorcast images. However, existing color restoration studies are limited in that they struggle to distinguish between haze and objects, particularly when haze veils and images have a similar color or when objects with a high saturation value occupy a significant portion of the scene, resulting in overly grayish images and distorted colors. Therefore, we propose a saturation-based dehazing method that extracts only the hue of the cast airlight and preserves the information of the object. First, the proposed color correction method uses a dominant color extraction method for the clustering of CIELAB(LAB) color images and then assigns area scores to the classified clusters. Sorting of the airlight areas is performed using the area score, and gray world-based white balance is performed by extracting the hue of the area. Finally, the saturation of the restored image is used to separate and process the distant objects and airlight, and dehazing is performed by applying a weighting value to the depth map based on the average luminance. Our color restoration method prevents excessive gray tone and color distortion. In particular, the proposed dehazing method improves upon existing issues where near-field information is lost and noise is introduced in the far field as visibility improves.
Assessing Food Safety Compliance in a Small-Scale Indian Food Manufacturer: Before and after Certification of the Food Safety Management System and Foreign Supplier Verification Program
Authors: Sasikumar Nair Surya , Mazurek-Kusiak Anna Katarzyna , Trafialek Joanna , Kolanowski Wojciech
Published: November 2023

The implementation of food safety management systems has a great influence on global food safety and security, especially for developing nations. The principles of the Hazard Analysis and Critical Control Points are crucial for food safety and are the basis of food safety management systems. The purpose of this study was to assess the food safety management systems of a selected small-sized food company in India, both before and after certification of the International Organization for Standardization (ISO) 22000 and the Foreign Supplier Verification Program (FSVP). This company manufactured traditional fried vegetable snacks using manual processing. The effectiveness of these certifications was assessed through direct audit. The integrated FSVP and ISO 22000 audit checklist were elaborated and applied in this study. It was shown that the certification significantly improved (97.14%) food safety system conformity in the company in question. Before certification, many non-compliances were found, especially for HACCP implementation. After certification, the only non-conformity concerned pest control. The integrated FSVP and ISO 22000 audit checklist used for the assessment enabled an evaluation of several factors: the food quality and safety compliance level, the effectiveness of certification, and the readiness of the small-sized Indian snack producer to develop the export of their products to the U.S. and other developed countries. It was shown that certification enabled a small-sized Indian food producer to meet the requirements of ISO 22000 and FSVP, which are the prerequisites for exporting to the U.S. and other developed countries.
A Decision Support System Based on the Integration of a Theory of Constraints and Strategic Management Tools for the Selection of Product Mixes
Authors: Elmas Yasin , Yüregir Hacire Oya , Yılmaz Ebru
Published: November 2023

The selection of products to be offered on the market is a critical decision-making process encountered in all industry sectors. It is not just a matter of maximizing profit or optimizing the utilization of corporate resources but also specifically concerns determining a product portfolio that is most suitably aligned to corporate strengths and that appeals to the most attractive and emerging markets. Hence, corporate competencies such as strategic management and production capabilities must be taken into account concurrently. Starting from this point of view, a twofold decision support system (DSS) has been developed. On the one hand, a theory of constraints (TOC)-based analytic hierarchy process (AHP) approach, including a taboo search algorithm, has been developed in order to derive the right product mix for maximizing the total profit amount by considering the bottleneck problem. On the other hand, a GE/McKinsey screen matrix is added to this consolidated approach to support decision-makers in the formulation of product portfolio strategies. The DSS provides a platform to compare outputs coming from the preceding two processes, which allows for the refinement of the solution. The proposed DSS is executed with a problem dataset from the industry to test its accuracy and reliability.
An Evaluation of Cesium Removal from Simulated Radioactive Contaminated Soil with a Magnetized Zeolite Derived from Anthracite
Authors: Zhang Yi , Zhu Xinyan , Gu Yu , Zhang Shengdong
Published: November 2023

The magnetized zeolite with an optimized conversion rate of 53% can be readily synthesized from industrial anthracite using a water-based method. The highly porous structure of ferromagnetism zeolite demonstrates robust magnetic properties with a magnetite content of 12–15%, satisfying adsorbent separation and recycling through magnetic cylinder rotating and vibrating separation. A cesium adsorption and removal efficiency as high as 99.92% with a corresponding adsorbent recovery ratio of up to 96.36% can be achieved for the simulated cesium-contaminated soil with a water content of 20% and a cesium content of 1% with an adsorbent-to-contaminated soil ratio of 1:2. Adsorption and magnetic separation technology with magnetized zeolite synthesized from anthracite exhibited a high cesium removal rate and zeolite recovery ratio, demonstrating promising application potential in treating radioactive waste soils and robust and economically viable engineering feasibility.
A Multidisciplinary Investigation of an Abandoned Old Mining Area Which Has Been Affected by the Combined Influences of Salt Karst and Human Exploration Activity
Authors: Szczerbowski Zbigniew , Gawałkiewicz Rafał
Published: November 2023

The authors discuss a case that is full of examples of the problems faced by civil engineers whose task is to develop areas in the face of natural, technological, or post-mining hazards. The study area is in the central part of Inowrocław, a town located on a massive salt dome of Zechstein salts. A strong deformation zone expanded in its upper part; this was caused by a natural process (related to so-called salt karst) and by mining activities that occurred in the past, creating a problem with regard to any potential spatial development in the town. The authors show a combination of data obtained using gravimetric and geodetic methods, which helps us to assess the geohazard risk. These include remote sensing data, which can be used to evaluate displacements of the ground surface. The authors used an approach that they term the Elevation Difference Method. This consists of determining displacements between ground surfaces: estimated on the basis of remote sensing data and on the basis of the historical data, when mounted measurement points (and remote sensing data) did not exist. The authors discuss the results in the light of the geological background. Within the area of the study, the displacements of the positive values dominate. The displacement occurs at 6 mm/yr on average and indicates diapiric uplift movement. The results are important for the town authorities for planning and development and for infrastructure management.
Leveraging Additive Manufacturing and Reverse Engineering for Circular Economy-Driven Remanufacturing of Hydraulic Drive System Components
Authors: Chiriță Alexandru-Polifron , Borș Adriana-Mariana , Rădoi Radu-Iulian , Dumitrescu Ionaș-Cătălin , Popescu Ana-Maria Carla
Published: November 2023

In response to the imperative of sustainability and resource efficiency, this article introduces an innovative framework for the remanufacture of hydraulic drive system components, firmly rooted in the tenets of the circular economy. This method mixes cutting-edge additive manufacturing technologies, meticulous 3D scanning techniques, and the rigorous discipline of reverse engineering to rebuild active elements within hydraulic drive systems. Fundamental to this framework is a resolute commitment to the repurposing of non-defective components, fostering a closed-loop system that systematically curtails waste generation and significantly diminishes the environmental footprint associated with hydraulic drive system maintenance. This article presents a meticulous examination of the remanufacturing process for a flowmeter, elucidating the intricate integration of additive manufacturing technology and the precision of 3D scanning to restore a previously damaged rotor. Through this interdisciplinary approach, we aim to elucidate the manner in which contemporary engineering practices can be harnessed to catalyze circularity, enhance resource efficiency, and increase sustainability within industrial operations. By demonstrating the efficacy of additive manufacturing and reverse engineering in hydraulic system remanufacturing, this article contributes significantly to the discourse on sustainable engineering methodologies, underlining their pivotal role in shaping a more environmentally conscientious, circular economy.
Using Augmented Small Multimodal Models to Guide Large Language Models for Multimodal Relation Extraction
Authors: He Wentao , Ma Hanjie , Li Shaohua , Dong Hui , Zhang Haixiang , Feng Jie
Published: November 2023

Multimodal Relation Extraction (MRE) is a core task for constructing Multimodal Knowledge images (MKGs). Most current research is based on fine-tuning small-scale single-modal image and text pre-trained models, but we find that image-text datasets from network media suffer from data scarcity, simple text data, and abstract image information, which requires a lot of external knowledge for supplementation and reasoning. We use Multimodal Relation Data augmentation (MRDA) to address the data scarcity problem in MRE, and propose a Flexible Threshold Loss (FTL) to handle the imbalanced entity pair distribution and long-tailed classes. After obtaining prompt information from the small model as a guide model, we employ a Large Language Model (LLM) as a knowledge engine to acquire common sense and reasoning abilities. Notably, both stages of our framework are flexibly replaceable, with the first stage adapting to multimodal related classification tasks for small models, and the second stage replaceable by more powerful LLMs. Through experiments, our EMRE2llm model framework achieves state-of-the-art performance on the challenging MNRE dataset, reaching an 82.95% F1 score on the test set.