bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of the Applicability of Thermochemical Processes for Solid Recovered Fuel
Authors: de la Torre-Bayo Juan Jesús , Zamorano Montserrat , Torres-Rojo Juan Carlos , Gil-Lalaguna Noemí , Gea Gloria , Fonts Isabel , Martín-Pascual Jaime
Published: November 2024

Within the context of the new circular model for wastewater treatment aimed at achieving zero waste, this research seeks an alternative to landfill disposal of waste screenings. It examines the feasibility of thermochemical processes—combustion and gasification—for the valorisation of solid recovered fuel (SRF) derived from screening wastes, which are the only waste in wastewater treatment plants (WWTPs) that typically have an absence of existing recycling or valorisation processes. Laboratory-scale experiments assessed the technical viability of gasification, and energy balances were calculated for both combustion and the syngas obtained from gasification experiments. Results indicate that both processes are feasible for SRF valorisation. Combustion demonstrated the highest energy efficiency, yielding up to 1.6 MJ per kg of raw SRF, compared to gasification’s maximum of 1.4 MJ. The moisture content in SRF feedstock influences both processes, underscoring the need to optimise moisture levels. Additionally, combustion showed a higher conversion efficiency due to the complete oxidation of the feedstock, whereas gasification produced valuable syngas that can be further utilised for energy production or as a chemical feedstock. The study concludes that, from a purely energetic perspective, combustion is the most efficient process for SRF valorisation. However, gasification offers significant environmental and sustainability advantages, including lower greenhouse gas emissions and the potential for integrating with renewable energy systems, making it a more attractive option for long-term sustainability goals.
EPR Spectroscopy Coupled with Spin Trapping as an Alternative Tool to Assess and Compare the Oxidative Stability of Vegetable Oils for Cosmetics
Authors: Di Prima Giulia , De Caro Viviana , Cardamone Cinzia , Oliveri Giuseppa , D’Oca Maria Cristina
Published: November 2024

Antioxidants are the most popular active ingredients in anti-aging cosmetics as they can restore the physiological radical balance and counteract the photoaging process. Instead of adding pure compounds into the formulations, some “precious” vegetable oils could be used due to their content of tocopherols, phenols, vitamins, etc., constituting a powerful antioxidant unsaponifiable fraction. Here, electron paramagnetic resonance (EPR) spectroscopy coupled with spin trapping was proven to provide a valid method for evaluating the antioxidant properties and the oxidative resistance of vegetable oils which, following UV irradiation, produce highly reactive radical species although hardly detectable. Extra virgin olive oil, sweet almond oil, apricot kernel oil, and jojoba oil were then evaluated by using N-t-butyl-α-phenylnitrone as a spin trapper and testing different UV irradiation times followed by incubation for 5 to 180 min at 70 °C. The EPR spectra were manipulated to obtain quantitative information useful for comparing the different tested samples. As a result, the knowledge acquired via the EPR analyses demonstrated jojoba oil as the best of the four considered oils in terms of both starting antioxidant ability and oxidative stability overtime. The obtained results confirmed the usefulness of the EPR spin trapping technique for the main proposed purpose.
Improving Mechanical Properties of Low-Quality Pure Aluminum by Minor Reinforcement with Fine B4C Particles and T6 Heat Treatment
Authors: Abishkenov Maxat , Tavshanov Ilgar , Lutchenko Nikita , Amanzholov Nursultan , Kalmyrzayev Daniyar , Ashkeyev Zhassulan , Nogaev Kayrosh , Kydyrbayeva Saltanat , Abdirashit Assylbek
Published: November 2024

Pure aluminum, due to its inherent low strength and softness, is unsuitable for most structural applications. However, unlike many aluminum alloys, pure aluminum exhibits high ductility and is often free from expensive alloying elements. This makes it a promising candidate for minor reinforcement to produce cost-effective composites with an optimal balance of strength and ductility. This study assesses the possibility of improving the mechanical performance of pure aluminum specimens by minor reinforcement (~0.36 wt. %) with fine B4C particles and T6 heat treatment. The composites were obtained using ultrasonic-assisted stir casting and were characterized by assessing their density, microhardness, yield strength (YS), ultimate tensile strength (UTS), and elongation. Light microscopy (LM), scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and X-ray diffraction (XRD) tests were conducted to investigate the presence and distribution of reinforcing particles in the Al matrix. Minor reinforcement of ~0.5–2 μm with B4C particles without/with subsequent T6 heat treatment resulted in an increase in microhardness by 71.45% and 143.37% and UTS by 71.05% and 140.16%, respectively, while the elongation values of the specimens decreased to 51.98% and 42.38%, respectively, compared with the adopted initial matrix Al specimen.
Correlational Research of Strength Parameters of Waste Soils Determined in the Laboratory and In Situ in Cracow
Authors: Zięba Jakub , Pilecka Elżbieta
Published: November 2024

This work presents an analysis of the relationship between strength parameters determined in the laboratory and the results of a cone penetration test with pore water pressure measurement (CPTU) of waste soils in the “White Seas” area in Cracow. Anthropogenic soil is an alkaline waste formed during the production of soda ash and deposited in the area of the former Solvay Sodium Plant factory in Cracow, Poland. Due to the large area of the land and numerous investment plans and completed buildings, there was a need to identify reliable functional relationships enabling the determination of the strength parameters of these soils based on the results of the CPTU. Statistical analysis showed that the best correlation with the test results was provided by two logarithmic functions in which the dependent variables were the effective friction angle and effective cohesion. The dependent variable for both cases was the corrected cone resistance qt. The functional relationship combined data from labour-intensive, long-lasting and costly laboratory measurements with quick and less expensive measurements, i.e., in situ CPTUs. The obtained relationships enable the determination of the strength properties of the subsoil of these anthropogenic soils.
Individualized, 3D Printed Matrices for the Reconstruction of Severely Destructed Teeth with Subgingival Margin—Case Series and Proof of Concept
Authors: Szabó Balázs , Néma Viktória , Jakab András , Braunitzer Gábor , Palkovics Dániel , Fráter Márk
Published: November 2024

Restoring deep, extensive carious lesions and subgingival situations can be challenging due to difficulties in placing matrices and achieving isolation. This article describes a technique utilizing individually designed digital matrices for challenging cases, offering an alternative solution aiding the reconstruction of the missing dental structures. Three cases are presented with difficult subgingival cavities or severe destruction of dental hard tissues, where a custom-made matrix was used to bridge the challenges of matrix placement and isolation. Digital impressions of dental arches were taken, and custom-made metal instruments were manufactured through computer-aided design and subsequent sintering. These instruments aided the elevation of deep margins and the reconstruction of the teeth. The presented technique yielded favorable results in terms of accuracy and feasibility for these challenging cases. However, it should be noted that the procedure requires additional time and incurs costs for the necessary elements.
Distraction Osteogenesis: A Comprehensive Review
Authors: Adejuyigbe Babapelumi , Gharpure Mohini , Wahle Charlotte F. , Kallini Jennifer R.
Published: November 2024

Distraction osteogenesis (DO) is a process which uses the bone’s natural healing tendencies to repair and lengthen pathologic, missing, or malformed bone. The mechanism of DO mimics the pathway that the body uses in any other fracture repair however the location of the fracture is carefully controlled by a surgical osteotomy. Postoperatively, the bone is allowed to begin its natural healing process, with the lengthener applying constant tension and thus re-initiating the process of healing along the length of the distraction gap. Current clinical indications for DO include limb length discrepancy, congenital bone length deformity, large bone defects, and extremity reconstruction due to hypoplasia or limb salvage procedures. The risks of DO include soft tissue complications, relapse or improper correction, cost or resource-related challenges, and psychosocial stigmas surrounding long treatment durations and the necessity of wearing the distraction lengthening hardware. Future directions for DO include supplements to the bone regeneration process (such as growth factors and/or mechanical stimulation) or improvements to the distractor device itself (changes in material and/or the structure of the device itself). This review aims to offer a comprehensive summary of the indications, underlying biological mechanisms, and practical considerations when implementing the use of distraction osteogenesis in clinical practice.
Association Between Weight-Adjusted Waist Index and Emphysema in Adults in the United States: A Cross-Sectional Study Involving 44,949 Participants
Authors: Cheng Hui , Yang Ziheng , Guo Jiateng , Zu Yukun , Li Fan , Zhao Bo
Published: November 2024

Background: The relationship between obesity and respiratory diseases has been widely explored. In this context, the Weight-Adjusted Waist Index (WWI) has emerged as a novel metric for assessing visceral fat. This study aims to evaluate the association between WWI and the risk of emphysema in the U.S. population, by utilizing data from the 2001–2018 National Health and Nutrition Examination Survey (NHANES). Methods: A cross-sectional study was conducted using NHANES data from 2001 to 2018. Logistic regression models were applied to assess the relationship between WWI and emphysema risk. Interaction and subgroup analyses were performed to explore effect modifiers. Results: Our study included a total of 44,949 American adults. The results of the multivariable logistic regression analysis revealed an association between WWI and the incidence of emphysema. In the fully adjusted model, the probability of developing emphysema was 1.5 times higher in the group with WWI > 10.46 compared to those with WWI ≤ 10.46, with an odds ratio of [1.5 (1.1, 1.9), p = 0.003]. Subgroup analysis showed stronger associations among males, non-Hispanic Whites, and individuals with hypertension. Furthermore, we used a two-piece linear regression model and found a nonlinear association between WWI and emphysema, with a breakpoint at 12.5. Conclusions: Our findings indicate a significant association between WWI levels and emphysema. Larger-scale prospective studies are needed to further explore the role of WWI in emphysema.
Application of Atomic Spectroscopy of Trapped Radioactive Ions in Nuclear Physics
Authors: de Groote Ruben P.
Published: November 2024

A review is given of precision measurements of hyperfine constants and nuclear g-factors measured with ions confined in ion traps. The nuclear physics observables which can be extracted from these types of measurements are discussed. The feasibility of future nuclear structure studies using precision atomic spectroscopy of trapped radioactive atoms, produced with accelerator-driven approaches, is discussed.
Accuracy Evaluation of a Three-Dimensional Face Reconstruction Model Based on the Hifi3D Face Model and Clinical Two-Dimensional Images
Authors: Xiao Yujia , Mao Bochun , Nie Jianglong , Liu Jiayi , Wang Shuo , Liu Dawei , Zhou Yanheng
Published: November 2024

Three-dimensional (3D) facial models have been increasingly applied in orthodontics, orthognathic surgery, and various medical fields. This study proposed an approach to reconstructing 3D facial models from standard orthodontic frontal and lateral images, providing an efficient way to expand 3D databases. A total of 23 participants (average age 20.70 ± 5.36 years) were enrolled. Based on the Hifi3D face model, 3D reconstructions were generated and compared with corresponding face scans to evaluate their accuracy. Root mean square error (RMSE) values were calculated for the entire face, nine specific facial regions, and eight anatomical landmarks. Clinical feasibility was further assessed by comparing six angular and thirteen linear measurements between the reconstructed and scanned models. The RMSE of the reconstruction model was 2.00 ± 0.38 mm (95% CI: 1.84–2.17 mm). High accuracy was achieved for the forehead, nose, upper lip, paranasal region, and right cheek (mean RMSE < 2 mm). The forehead area showed the smallest deviation, at 1.52 ± 0.88 mm (95% CI: 1.14–1.90 mm). In contrast, the lower lip, chin, and left cheek exhibited average RMSEs exceeding 2 mm. The mean deviation across landmarks was below 2 mm, with the Prn displaying the smallest error at 1.18 ± 1.10 mm (95% CI: 0.71–1.65 mm). The largest discrepancies were observed along the Z-axis (Z > Y > X). Significant differences (p < 0.05) emerged between groups in the nasolabial, nasal, and nasofrontal angles, while the other 13 linear and 3 angular measurements showed no statistical differences (p > 0.05). This study explored the feasibility of reconstructing accurate 3D models from 2D photos. Compared to facial scan models, the Hifi3D face model demonstrated a 2 mm deviation, with potential for enriching 3D databases for subjective evaluations, patient education, and communication. However, caution is advised when applying this model to clinical measurements, especially angle assessments.
Stem and Progenitor Cells for Musculoskeletal Disease Modeling and Tissue Repair
Authors: He Songlin , Niu Wanting , Li Zhong Alan
Published: November 2024

Musculoskeletal conditions such as osteoarthritis (OA), bone fracture, and sarcopenia are highly prevalent [...]
Evaluation of BRIP-1 (FANCJ) and FANCI Protein Expression in Ovarian Cancer Tissue
Authors: Kozłowski Mateusz , Borzyszkowska Dominika , Golara Anna , Durys Damian , Piotrowska Katarzyna , Kempińska-Podhorodecka Agnieszka , Cymbaluk-Płoska Aneta
Published: November 2024

Background: Ovarian cancer is one of the most common cancers in women. Markers associated with ovarian cancer are still being sought. The aim of this study was to evaluate the expression of BRIP-1 (FANCJ) and FANCI proteins in ovarian cancer tissue and to assess these expressions in differentiating the described clinical features. Methods: The study enrolled 68 patients with ovarian cancer. The cohort was divided into a HGSOC (high-grade serous ovarian cancer) group and a non-HGSOC group, which represented ovarian cancer other than HGSOC. Immunohistochemical evaluation of FANCI and BRIP-1 (FANCJ) protein expression in ovarian cancer tissue samples was performed. All statistical analyses were performed using StatView software (Carry, NC, USA). Results: The FANCI protein mostly showed moderate positive and strong positive expression, while BRIP-1 protein mostly showed no expression or positive expression. Patients with lower expression of FANCI and BRIP-1 showed differences in the clinical stage of HGSOC, which was not observed in patients with higher expression of these proteins. In addition, patients with lower BRIP-1 expression showed differences in menopausal status, which was not observed in patients with higher expression of this protein. Conclusions: This study shows that FANCI protein is a marker associated with lower FIGO stage and histologically high-grade cancer in a group of all ovarian cancers and in non-HGSOC.
Advances in Surface-Enhanced Raman Spectroscopy for Urinary Metabolite Analysis: Exploiting Noble Metal Nanohybrids
Authors: Zhao Ningbin , Shi Peizheng , Wang Zengxian , Sun Zhuang , Sun Kaiqiang , Ye Chen , Fu Li , Lin Cheng-Te
Published: November 2024

This review examines recent advances in surface-enhanced Raman spectroscopy (SERS) for urinary metabolite analysis, focusing on the development and application of noble metal nanohybrids. We explore the diverse range of hybrid materials, including carbon-based, metal–organic-framework (MOF), silicon-based, semiconductor, and polymer-based systems, which have significantly improved SERS performance for detecting key urinary biomarkers. The principles underlying SERS enhancement in these nanohybrids are discussed, elucidating both electromagnetic and chemical enhancement mechanisms. We analyze various fabrication methods that enable precise control over nanostructure morphology, composition, and surface chemistry. The review critically evaluates the analytical performance of different hybrid systems for detecting specific urinary metabolites, considering factors such as sensitivity, selectivity, and stability. We address the analytical challenges associated with SERS-based urinary metabolite analysis, including sample preparation, matrix effects, and data interpretation. Innovative solutions, such as the integration of SERS with microfluidic devices and the application of machine learning algorithms for spectral analysis, are highlighted. The potential of these advanced SERS platforms for point-of-care diagnostics and personalized medicine is discussed, along with future perspectives on wearable SERS sensors and multi-modal analysis techniques. This comprehensive overview provides insights into the current state and future directions of SERS technology for urinary metabolite detection, emphasizing its potential to revolutionize non-invasive health monitoring and disease diagnosis.
The Biotechnological Potential of Crickets as a Sustainable Protein Source for Fishmeal Replacement in Aquafeed
Authors: Fraijo-Valenzuela Aldo , Arias-Moscoso Joe Luis , García-Pérez Oscar Daniel , Rodriguez-Anaya Libia Zulema , Gonzalez-Galaviz Jose Reyes
Published: November 2024

As aquaculture production grows, so does the demand for quality and cost-effective protein sources. The cost of fishmeal (FM) has increased over the years, leading to increased production costs for formulated aquafeed. Soybean meal (SBM) is commonly used as an FM replacer in aquafeed, but anti-nutritional factors could affect the growth, nutrition, and health of aquatic organisms. Cricket meal (CM) is an alternative source with a nutrient profile comparable to FM due to its high protein content, digestibility, and amino acid profile. CM use in aquafeed influences growth and reproductive performance while modulating the gut microbiota and immune response of fish and shrimp. However, consistent regulation and scaling up are necessary for competitive prices and the marketing of CM. Moreover, the chitin content in CM could be an issue in some fish species; however, different strategies based on food biotechnology can improve the protein quality for its safe use in aquafeed.
AI Threats to Politics, Elections, and Democracy: A Blockchain-Based Deepfake Authenticity Verification Framework
Authors: Islam Masabah Bint E. , Haseeb Muhammad , Batool Hina , Ahtasham Nasir , Muhammad Zia
Published: November 2024

The integrity of global elections is increasingly under threat from artificial intelligence (AI) technologies. As AI continues to permeate various aspects of society, its influence on political processes and elections has become a critical area of concern. This is because AI language models are far from neutral or objective; they inherit biases from their training data and the individuals who design and utilize them, which can sway voter decisions and affect global elections and democracy. In this research paper, we explore how AI can directly impact election outcomes through various techniques. These include the use of generative AI for disseminating false political information, favoring certain parties over others, and creating fake narratives, content, images, videos, and voice clones to undermine opposition. We highlight how AI threats can influence voter behavior and election outcomes, focusing on critical areas, including political polarization, deepfakes, disinformation, propaganda, and biased campaigns. In response to these challenges, we propose a Blockchain-based Deepfake Authenticity Verification Framework (B-DAVF) designed to detect and authenticate deepfake content in real time. It leverages the transparency of blockchain technology to reinforce electoral integrity. Finally, we also propose comprehensive countermeasures, including enhanced legislation, technological solutions, and public education initiatives, to mitigate the risks associated with AI in electoral contexts, proactively safeguard democracy, and promote fair elections.
Galantamine-Memantine Combination in the Treatment of Parkinson’s Disease Dementia
Authors: Frost Emma D. , Shi Swanny X. , Byroju Vishnu V. , Pitton Rissardo Jamir , Donlon Jack , Vigilante Nicholas , Murray Briana P. , Walker Ian M. , McGarry Andrew , Ferraro Thomas N. , Hanafy Khalid A. , Echeverria Valentina , Mitrev Ludmil , Kling Mitchel A. , Krishnaiah Balaji , Lovejoy David B. , Rahman Shafiqur , Stone Trevor W. , Koola Maju Mathew
Published: November 2024

Parkinson’s disease (PD) is a progressive neurodegenerative disorder that affects over 1% of population over age 60. It is defined by motor and nonmotor symptoms including a spectrum of cognitive impairments known as Parkinson’s disease dementia (PDD). Currently, the only US Food and Drug Administration-approved treatment for PDD is rivastigmine, which inhibits acetylcholinesterase and butyrylcholinesterase increasing the level of acetylcholine in the brain. Due to its limited efficacy and side effect profile, rivastigmine is often not prescribed, leaving patients with no treatment options. PD has several derangements in neurotransmitter pathways (dopaminergic neurons in the nigrostriatal pathway, kynurenine pathway (KP), acetylcholine, α7 nicotinic receptor, and N-methyl-D-aspartate (NMDA) receptors) and rivastigmine is only partially effective as it only targets one pathway. Kynurenic acid (KYNA), a metabolite of tryptophan metabolism, affects the pathophysiology of PDD in multiple ways. Both galantamine (α7 nicotinic receptor) and memantine (antagonist of the NMDA subtype of the glutamate receptor) are KYNA modulators. When used in combination, they target multiple pathways. While randomized controlled trials (RCTs) with each drug alone for PD have failed, the combination of galantamine and memantine has demonstrated a synergistic effect on cognitive enhancement in animal models. It has therapeutic potential that has not been adequately assessed, warranting future randomized controlled trials. In this review, we summarize the KYNA-centric model for PD pathophysiology and discuss how this treatment combination is promising in improving cognitive function in patients with PDD through its action on KYNA.
An Evidence-Driven Approach to Slip and Fall Prevention in Large Campus Facilities
Authors: Chew Michael Yit Lin , Asmone Ashan Senel , Lam Mark Tiam Weng
Published: November 2024

We developed an evidence-based risk assessment and benchmarking framework towards pedestrian safety. Pendulum slip resistance tests were conducted on 23 sites within a large campus facility covering ceramic tiles, pebbles, tactile indicators, and metal coverings for manholes and drainage. The results show frictional resistance can be reduced when tested wet and exacerbated when it is on a slope. The results were further verified via laboratory tests under controlled conditions. The perceived affordance of certain features such as tactile indicators providing a better grip or traction requires urgent attention. Therefore, a data-driven approach not only enhances the accuracy of slip risk assessments but also establishes empirically grounded benchmarks for surface safety, ensuring effective and resource-efficient interventions. The findings contribute to the existing body of knowledge and future research agenda in pedestrian safety, offering a robust foundation for benchmarking and risk management efforts in diverse environments.
Correction: Akbar et al. Seismic Response Compression of Various MSE Walls Based on 3D Modeling. Buildings 2023, 13, 2773
Authors: Akbar Muhammad , Pan Huali , Ou Guoqiang , Nikitas Georgios , Ahmed Bilal
Published: November 2024

In the published publication [...]
Direct Tensile Test Method for Shotcrete
Authors: Kabancev Oleg V. , Simakov Oleg A.
Published: November 2024

This study substantiates the need for direct tensile strength testing of shotcrete and fiber-reinforced shotcrete, rather than relying on indirect methods, to accurately reflect material performance under biaxial stress conditions when used for structural reinforcement. Experiments on field specimens confirmed that tensile strength values derived through direct testing differ significantly from those calculated based on compressive strength. The study presents a new testing methodology with optimized specimen dimensions (32, 40, 50, and 82 mm diameter cylinders with length-to-diameter ratios of 3.0) to mitigate eccentricity effects, ensuring normal-section failure. Results show that tensile strength values for fiber-reinforced shotcrete with brass-coated fibers (13–15 mm length, 0.3–0.5 mm diameter, 30 kg/m3 dosage) reached 68 MPa, compared to 60 MPa for standard shotcrete, while basalt-fiber reinforcement (6 mm length, 1% by weight) resulted in 42 MPa. The initial modulus of elasticity for unreinforced shotcrete was 280 × 103 MPa, with fiber reinforcement slightly increasing this value to 287 × 103 MPa. The findings support a direct approach to testing, providing a foundation for developing predictive methodologies for fiber-reinforced shotcrete properties based on reinforcement type and dosage. These results are essential for applications such as seismic strengthening, where accurate tensile characteristics are critical for performance under dynamic loading.
Review of Big Data Implementation and Expectations in Smart Cities
Authors: Zhuang Yingnan , Cenci Jeremy , Zhang Jiazhen
Published: November 2024

With the construction of smart cities advancing, research on big data and smart cities has become crucial for sustainable development. This study seeks to fill gaps in the literature and elucidate the significance of big data and smart city research, offering a comprehensive analysis that aims to foster academic understanding, promote urban development, and drive technological innovation. Using bibliometric methods and Citespace software (6.2.R3), this study comprehensively examines the research landscape from 2015 to 2023, aiming to understand its dynamics. Under the guidance of the United Nations, global research on big data and smart cities is progressing. Using the Web of Science (WOS) Core Collection as the data source, an exhaustive visual analysis was conducted, revealing various aspects, including the literature output, journal distribution, geographic study trends, research themes, and collaborative networks of scholars and institutions. This study reveals a downward trend despite research growth from 2015 to 2020, focusing on digital technology, smart city innovations, energy management and environmental applications, data security, and sustainable development. However, biases persist towards technology, information silos, homogenised research, and short-sighted strategies. Research should prioritise effectiveness, applications, diverse fields, and interdisciplinary collaboration to advance smart cities comprehensively. In the post-COVID-19 era, using big data to optimise city management is key to fostering intelligent, green, and humane cities and to exploring efficient mechanisms to address urban development challenges in the new era.
Intratumoral Treatment in Lung Cancer: Is It Time to Move Towards Clinical Practice?
Authors: Pagliari Gabriele Giuseppe , Colonese Francesca , Canova Stefania , Abbate Maria Ida , Sala Luca , Petrella Francesco , Clementi Thoma Dario , Cortinovis Diego Luigi
Published: November 2024

According to a modern view, cancer no longer follows a purely mechanistic model. Rather, a tumor is conceived as a more complex structure, composed of cancer cells, the activities of which may interact and reshape the so-called tumor microenvironment (TME), leading to preservation of specific tumoral niches and promoting the survival of tumoral stem cells. Background/Objective: Therapeutic strategies must deal with this unique cancer architecture in the near future by widening their range of activities outside the cancer cells and rewiring a TME to ensure it is hostile to cancer growth. Therefore, an intratumoral therapeutic strategy may open the door to a new type of anticancer activity, one that directly injures the tumoral structure while also eliciting an influence on the TME through local and systemic immunomodulation. This review would like to assess the current situation of intratumoral strategies and their clinical implications. Methods We analyzed data from phase I, II, and III trials, comprehensive reviews and relevant clinical and preclinical research, from robust databases, like PUBMED, EMBASE, Cochrane Library, and clinicaltrials.gov. Results: Intratumoral strategies can be quite variable. It is possible the injection and inhalation of traditional antiblastic agents or immunomodulant agents, or intrapleural administration. Ablation strategy is available, both thermal and photodynamic method. Moreover, TTfields and NPs are analyzed and also brachytherapy is mentioned. Intratumoral therapy can find space in “adjuvant”/perioperative or metastatic settings. Finally, intratumoral strategies allow to synergize their activities with systemic therapies, guaranteeing better local and systemic disease control. Conclusions: Intratumoral strategies are overall promising. Antiblastic/immunomodulant injection and NPs use are especially interesting and intriguing. But, there is generally a lack of phase II and III trials, in particular NPs use need additional experimentation and clinical studies.
The Value of Repeat 5-HIAA Measurements as a Predictor of Carcinoid Heart Disease: A Prospective 5-Year Follow-Up Study in Patients with Small Intestinal Neuroendocrine Tumors
Authors: Kostiainen Iiro , Simonen Piia , Aaltonen Katri , Lindén Riikka , Karppinen Noora , Gordin Daniel , Rapola Janne , Schalin-Jäntti Camilla , Matikainen Niina
Published: November 2024

Background: Small intestinal neuroendocrine tumors (SI-NETs) are characterized by carcinoid syndrome and carcinoid heart disease (CHD). The aim of the present study was to identify early risk markers for carcinoid heart disease and survival in a prospective median-term follow-up setting. Methods: We measured 5-HIAA and cumulative 5-HIAA exposure (Cum-5-HIAA) based on repeated measurements, proBNP, vascular function, hepatic tumor load, and transthoracic echocardiography (TTE) at baseline and during the median 5-year follow-up. Of 65 patients with SI-NETs, 54 patients underwent a prospective follow-up. In addition, survival was evaluated during the median follow-up of 6 years. Results: At baseline, three patients had CHD. During the median follow-up of 5 years, two patients (4%) developed CHD. Cum-5-HIAA and proBNP correlated with CHD (Westberg score, Spearman’s ρ = 0.32 and 0.31, respectively). Cum-5-HIAA had a superior diagnostic capability, predicting CHD in receiver operator characteristic analysis with an AUC of 0.98 (95% CI: 0.94–1.00) and outperformed proBNP, chromogranin A (CgA), and individual serum 5-HIAA measurements (AUC = 0.75, 0.85, and 0.91, respectively). Minor changes in valve regurgitation were frequently detected but did not correlate with vascular function. Regurgitation increased or decreased in 29% of tricuspid and 30% of pulmonic valves. CHD, hepatic tumor load, serum 5-HIAA, and elevated aortic pulse wave velocity (PWV) were associated with increased mortality in SI-NET patients. Conclusions: Cum-5-HIAA is a promising biomarker for CHD risk and outperformed other biomarkers. CHD and hepatic tumor load are the strongest predictors of mortality. PWV is a novel predictor of survival. The incidence of CHD was low among the SI-NET patients, probably reflecting successful treatment regimens.
Streamline Flow of the Portal Vein Affects the Distribution of Colorectal Cancer Metastases: Clinical Reality or Just a Belief? A Systematic Review and Meta-Analysis
Authors: Savvakis Stavros , Lagopoulos Vasileios I. , Mantalovas Stylianos , Paschou Eleni , Kopsidas Periklis , Sevva Christina , Karakousis Alexandros Vasileios , Gigi Eleni , Kesisoglou Isaak
Published: November 2024

Background: The “streamline flow” of the portal vein (PV) is a controversial yet well-known theory presented almost 125 years ago. It states that blood from the superior mesenteric vein (SMV) goes preferentially to the right liver lobe, while splenic and inferior mesenteric veins divert blood predominantly to the left lobe, affecting this way the metastatic distribution of colorectal cancer (CRC). The aim of this systematic review is to assess its validity by examining and combining all the relevant literature with a critical eye. Materials and methods: This study constitutes a systematic review of the literature and adheres to all PRISMA system criteria. Three search engines (PubMed, Google Scholar, and the Cochrane Library) were used, and 11 out of 435 studies between 1984 and 2024 were finally included in this meta-analysis. All statistical analysis was conducted using RevMan Web, Version: 7.12.0, and Jamovi v 2.3.260 software. Results: The meta-analysis revealed that regardless of the primary location of the tumor, the metastases preferentially migrate to the right lobe due to various possible reasons. The prevalence of metastases from right colon cancer to the right liver lobe was 75%, while the prevalence of left colon cancer metastases to the right lobe was 68%. This difference was not found statistically significant. Conclusions: The theory has been proven inaccurate, at least from the perspective of the origin-associated distribution of CRC metastases in the liver lobes, and therefore bears no clear predictive value. Further research under different perspectives is essential for determining more definite conclusions.
Synthesis and Experimental Screening of Catalysts for H2S to H2 Decomposition Under Close-to-Industry Conditions
Authors: Palankoev Timur , Manakhov Anton , Kovalskii Andrey , Sukhanova Ekaterina , Popov Zakhar , Chareev Dmitry , Dement’ev Konstantin , Maximov Anton , Al-Qasim Abdulaziz
Published: November 2024

The chemical engineering community has shown significant interest in investigating methods to decompose hydrogen sulfide into hydrogen and sulfur. However, there is still a lack of detailed experimental data enabling us to choose the optimal catalyst, reaction, and regeneration conditions, as well as the overall process design. The purpose of this work is to synthesize a series of catalysts and compare their catalytic activity under the same conditions, chosen on the basis of a possible large-scale H2S conversion process. To achieve this, the obtained catalysts were characterized by BET, XRD, SEM, TEM, and XPS before and after the reaction. Decomposition was conducted in a laboratory fixed-bed reactor at a temperature of 500 °C, 10 vol% of H2S in the feed, and a GHSV of 540–1000 h−1. DFT calculations evaluated the H2S bond cleavage on various catalyst surfaces. It was shown that the most promising catalyst was Ni3S2, offering an acceptable H2S conversion of 40%. We also observed that Ni3S2 catalyst regeneration could be conducted at much milder conditions compared to those previously reported in the literature. These results highlight the viability of upscaling the process with the selected catalyst.
Clinical Characteristics and Prognosis of Hemolytic Disease of the Newborn Caused by Irregular Antibodies: A 13-Year Retrospective Analysis
Authors: Wu Hui , Li Rui , Wei Hongling , Zhu Weiwei , Xing Yan
Published: November 2024

Background/Objectives: The clinical characteristics and outcomes of hemolytic disease of the newborn (HDN) caused by irregular antibodies remain unclear. Herein, we analyzed the clinical features and prognosis of HDN. Methods: Children admitted to our institution between June 2009 and December 2022 with a definite diagnosis of HDN were evaluated. Patients with irregular antibodies were matched in a 1:3 ratio to those with ABO incompatibility. Children with confirmed Rh-incompatibility hemolytic disease were divided into the RhD subgroup (hemolysis induced by Rh anti-D) and the non-RhD group (hemolysis induced by other Rh antibodies). Results: The irregular antibody and ABO incompatibility group included 32 and 96 patients, respectively. Compared to the ABO incompatibility group, the irregular antibody group showed earlier jaundice; higher incidence of liver and spleen enlargement and anemia; higher direct antiglobulin test (DAT) positivity; earlier and more severe anemia; higher rates of enhanced phototherapy, blood transfusion, and blood exchange; and longer hospital stay (all p < 0.05). Compared to the non-RhD group, the RhD subgroup showed an earlier occurrence of jaundice and a higher incidence of liver and spleen enlargement (both p < 0.05). The multiple irregular antibody subgroup further showed earlier occurrence of jaundice and a higher rate of enhanced phototherapy, blood transfusion, and blood exchange than the single-antibody group (both p < 0.05). Conclusions: HDN caused by irregular red blood cell antibodies is rare, but clinical manifestations are serious. It is important to pay attention to the screening of irregular antibodies during pregnancy, to strengthen monitoring, and to provide intrauterine treatment and early intervention as necessary.
Extracellular Vesicles: A Novel Diagnostic Tool and Potential Therapeutic Approach for Equine Osteoarthritis
Authors: Elashry Mohamed I. , Speer Julia , De Marco Isabelle , Klymiuk Michele C. , Wenisch Sabine , Arnhold Stefan
Published: November 2024

Osteoarthritis (OA) is a chronic progressive degenerative joint disease that affects a significant portion of the equine population and humans worldwide. Current treatment options for equine OA are limited and incompletely curative. Horses provide an excellent large-animal model for studying human OA. Recent advances in the field of regenerative medicine have led to the exploration of extracellular vesicles (EVs)—cargoes of microRNA, proteins, lipids, and nucleic acids—to evaluate their diagnostic value in terms of disease progression and severity, as well as a potential cell-free therapeutic approach for equine OA. EVs transmit molecular signals that influence various biological processes, including the inflammatory response, apoptosis, proliferation, and cell communication. In the present review, we summarize recent advances in the isolation and identification of EVs, the use of their biologically active components as biomarkers, and the distribution of the gap junction protein connexin 43. Moreover, we highlight the role of mesenchymal stem cell-derived EVs as a potential therapeutic tool for equine musculoskeletal disorders. This review aims to provide a comprehensive overview of the current understanding of the pathogenesis, diagnosis, and treatment strategies for OA. In particular, the roles of EVs as biomarkers in synovial fluid, chondrocytes, and plasma for the early detection of equine OA are discussed.
Selenized Yeast Protects Against Cadmium-Induced Follicular Atresia in Laying Hens by Reducing Autophagy in Granulosa Cells
Authors: Wu Caimei , Jiang Yuxuan , Zhou Ziyun , Zhang Yuwei , Zhou Yixuan , Bai Shiping , Li Jian , Wu Fali , Wang Jianping , Lyu Yang
Published: November 2024

Cadmium (Cd) exposure can induce follicular atresia and laying performance reduction in hens, which is linked to autophagy within the granulosa cells. Selenium (Se) can influence autophagy and counteract Cd toxicity. This study aimed to investigate the protective effect of Se on Cd-induced follicular atresia in laying hens. Sixty-four laying hens were randomly allocated into 4 treatments: control group: basal diet; Se group: basal diet + 0.4 mg/kg Se from selenized yeast; Cd group: basal diet + 25 mg/kg Cd from CdCl2; and Cd+Se group: basal diet + 25 mg/kg Cd + 0.4 mg/kg Se. Compared to the Cd group, Se supplementation alleviated the ovarian pathological changes and oxidative stress in the follicles, serum, liver, and ovary, increased daily laying production, ovarian weight and F5–F1 follicle amounts, serum levels of progesterone and oestradiol, and up-regulated mTOR expression (p < 0.05), while decreasing the count of autophagic vacuoles, ovarian atresia follicle numbers, and Cd deposition, and down-regulated expression levels of autophagy-related mRNAs, including ATG5, LC3-I, and LC3-II, Beclin1, and Dynein in the follicles (p < 0.05). In conclusion, 0.4 mg/kg Se supplementation protected against Cd-induced laying performance reduction and follicular atresia, which were achieved via decreasing oxidative stress and inhibiting mTOR pathways of autophagy.
Pharmacological and Biological Targeting of FGFR1 in Cancer
Authors: Fan Shuai , Chen Yuxin , Wang Wenyu , Xu Wanting , Tian Mei , Liu Yuetong , Zhou Yutong , Liu Dan , Xia Qin , Dong Lei
Published: November 2024

FGFR1 is a key member of the fibroblast growth factor receptor family, mediating critical signaling pathways such as RAS-MAPK and PI3K-AKT. which are integral to regulating essential cellular processes, including proliferation, differentiation, and survival. Alterations in FGFR1 can lead to constitutive activation of signaling pathways that drive oncogenesis by promoting uncontrolled cell division, inhibiting apoptosis, and enhancing the metastatic potential of cancer cells. This article reviews the activation mechanisms and signaling pathways of FGFR1 and provides a detailed exposition of the types of FGFR1 aberration. Furthermore, we have compiled a comprehensive overview of current therapies targeting FGFR1 aberration in cancer, aiming to offer new perspectives for future cancer treatments by focusing on drugs that address specific FGFR1 alterations.
Ornamental Barberry Twigs as an Underexploited Source of Berberine-Rich Extracts—Preliminary Research
Authors: Miłek Michał , Dżugan Małgorzata , Pieńkowska Natalia , Galiniak Sabina , Mołoń Mateusz , Litwińczuk Wojciech
Published: November 2024

Berberine is a natural substance obtained from the roots of common barberry which, due to its strong pharmacological activity, is a commonly tested ingredient of dietary supplements. However, ornamental barberries, which are widely available, have not been considered as a source of berberine so far. The research aimed to check whether the ornamental barberry leaves and twigs could be used as an easily accessible raw material for obtaining natural berberine-rich extract with biological activity. Twigs and leaves of seven cultivars of ornamental barberry extracts were assessed for their polyphenol content, antioxidant potential (FRAP and DPPH), and berberine content using high-performance thin layer chromatography (HPTLC). As a reference, commercially available roots of Berberis vulgaris were used. For the next step, selected extracts (two with high and two with low berberine content) were tested on three cell lines (HaCaT, A375, Caco-2) using neutral red assay, and pure berberine sulfate (1–100 μg mL−1) was used as a control. Although the antioxidant potential of aqueous–methanol extracts of tested barberry was higher for the leaves than for the twigs, the berberine content was determined only in the twig extracts (from 42 to 676 mg 100 g−1). Studies on cell lines have shown the general toxicity of barberry extracts, but the observed effect was not directly correlated with the content of the alkaloid. However, the extract showed greater activity compared to an analogous dose of pure berberine, suggesting a significant effect of the matrix composition. For the first time, it was shown that the twigs of selected cultivars of ornamental barberry can be considered as a promising berberine source for the pharmaceutical industry to develop new effective formulations. However, these findings require further studies.
RNA Splicing Aberrations in Hereditary Cancer: Insights from Turkish Patients
Authors: Kilic Seda , Sukruoglu Erdogan Ozge , Tuncer Seref Bugra , Celik Demirbas Betul , Yalniz Kayim Zubeyde , Yazici Hulya
Published: November 2024

The process of RNA splicing is fundamental in contributing to proteomic diversity and regulating gene expression. Dysregulation of splicing is associated with various human disorders, including cancer. Through functional studies, this study sought to examine the potential impact of seven variants within six inherited cancer-related genes on RNA splicing patterns in Turkish cancer patients. Upon detecting variants using Next-Generation Sequencing (NGS), we used Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and Sanger sequencing to elucidate the effects of these variants on splicing. Three of the seven variants demonstrated no discernible effect on RNA, while four exhibited pathogenic characteristics. Specifically, the variants APC c.532-1G>A rs1554072547, BRCA1c.4358-3A>G rs1567779966, BRCA2c.7436-1G>C rs81002830 and MSH3c.1897-1G>A rs1744149615 were identified as pathogenic, while the variants BLMc.4076+4T>G rs183176301, RB1c.2489+2T>C rs1555294636 and RB1c.1050-2A>G rs? were found to be benign from a splicing perspective. These findings highlight the importance of verifying the precise consequences of splice-site variants through experimental analysis, given their potential implications for genetic disorders and cancer predisposition. This research contributes to the understanding of splice-site variants in inherited cancer predisposition, particularly among Turkish cancer patients. It emphasizes the necessity for further exploration into the mechanisms and functional consequences of alternative splicing for potential therapeutic interventions in cancer.