bluuebunny/crossref_metadata_2025 huggingface dataset:
In Vitro Influence of ZnO, CrZnO, RuZnO, and BaZnO Nanomaterials on Bacterial Growth
Authors: Abdallah Emad M. , Modwi Abueliz , Al-Mijalli Samiah H. , Mohammed Afrah E. , Idriss Hajo , Omar Abdulkader Shaikh , Afifi Mohamed , AL-Farga Ammar , Goh Khang Wen , Ming Long Chiau
Published: November 2022

In this work, ZnO, CrZnO, RuZnO, and BaZnO nanomaterials were synthesized and characterized in order to study their antibacterial activity. The agar well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays were used to determine the antibacterial activity of the fabricated nanomaterials against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC35218, Klebsiella pneumoniae ATCC 7000603, and Pseudomonas aeruginosa ATCC 278533. The well-diffusion test revealed significant antibacterial activity against all investigated bacteria when compared to vancomycin at a concentration of 1 mg/mL. The most susceptible bacteria to BaZnO, RuZnO, and CrZnO were Staphylococcus aureus (15.5 ± 0.5 mm), Pseudomonas aeruginosa (19.2 ± 0.5 mm), and Pseudomonas aeruginosa (19.7 ± 0.5), respectively. The MIC values indicated that they were in the range of 0.02 to 0.2 mg/mL. The MBC values showed that the tested bacteria’s growth could be inhibited at concentrations ranging from 0.2 to 2.0 mg/mL. According to the MBC/MIC ratio, BaZnO, RuZnO, and CrZnO exhibit bacteriostatic effects and may target bacterial protein synthesis based on the results of the tolerance test. This study shows the efficacy of the above-mentioned nanoparticles on bacterial growth. Further biotechnological and toxicological studies on the nanoparticles fabricated here are recommended to benefit from these findings.
In Vitro Anti-Influenza A Virus H1N1 Effect of Sesquiterpene-Rich Extracts of Carpesium abrotanoides
Authors: Li Li , Yang Shenghui , Chen Dilu , Wu Zhihuang , Zhang Meijun , Yang Fang , Qin Li , Zhou Xiaojiang
Published: November 2022

Due to a high content of sesquiterpenes, Carpesium abrotanoides has been investigated to fully explore its health-promoting properties. Therefore, this work aimed to assess, for the first time, the anti-influenza A virus H1N1 potential of sesquiterpene-targeted fractions of the herb derived from C. abrotanoides. Five compounds, including four sesquiterpenes and one aldehyde, were isolated and identified from the sesquiterpene-rich extracts of C. abrotanoides (SECA), and the contents of three main sesquiterpenes in the SECA were determined. Furthermore, SECA showed a significant protective effect in the MDCK cells infected with influenza A virus (H1N1) in three different conditions: premixed administration, prophylactic administration, and therapeutic administration. SECA can significantly decrease the mRNA expressions of TLR4, MyD88, NF-κB, TNF-α, and IL-6, as well as the protein expressions of TLR4, MyD88, and NF-κB. This result suggests that SECA can resist the influenza A virus H1N1 through the TLR4/MyD88/NF-κB signal pathway.
Anticonvulsant Activity of Bombyx batryticatus and Analysis of Bioactive Extracts Based on UHPLC-Q-TOF MS/MS and Molecular Networking
Authors: Wang Qinglei , Wang Rong , Zheng Cheng , Zhang Linlin , Meng Hong , Zhang Yi , Ma Linke , Chen Bilian , Wang Juanjuan
Published: November 2022

Bombyx batryticatus (BB) is an anticonvulsant animal medicine in traditional Chinese medicine (TCM) and acts on the central nervous system. This research aimed to study the anticonvulsant effects of different polarity fractions of extracts from BB and to explore the components conferring anticonvulsant activity. Materials and methods: Crude extracts of BB at 20 g/kg were divided into different polarity fractions (petroleum ether, chloroform, ethyl acetate, water) and were administered to groups of mice before injecting pentylenetetrazol (PTZ) to induce convulsions. The animals were placed in chambers, and their behaviors were recorded for 30 min following the injection. Latency time, percent of protection, convulsion, convulsion rate, and convulsion score were determined for these mice. The compounds present in the different fractions were analyzed, and those from the fraction that conferred anticonvulsant activity were identified by high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF MS) and molecular networking (MN). The chloroform extract fractions (B-C) clearly increased the seizure latency time and protection percentage and decreased the convulsion percentage compared to the control group. The anticonvulsant effect of other extract fractions was not significant. Our study shows that the chloroform extract fractions (B-C) of BB have a significant anticonvulsant effect. We also identified 17 compounds including lumichrome, pheophorbide A, and episyringaresinol 4′-O-beta-d-glucopyranose that were found for the first time. The results of this study may lay the groundwork for studying compounds derived from Bombyx batryticatus and their anticonvulsant effect.
Parenteral Nanoemulsions Loaded with Combined Immuno- and Chemo-Therapy for Melanoma Treatment
Authors: Monge Chiara , Stoppa Ian , Ferraris Chiara , Bozza Annalisa , Battaglia Luigi , Cangemi Luigi , Miglio Gianluca , Pizzimenti Stefania , Clemente Nausicaa , Gigliotti Casimiro Luca , Boggio Elena , Dianzani Umberto , Dianzani Chiara
Published: November 2022

High-grade melanoma remains a major life-threatening illness despite the improvement in therapeutic control that has been achieved by means of targeted therapies and immunotherapies in recent years. This work presents a preclinical-level test of a multi-pronged approach that includes the loading of immunotherapeutic (ICOS-Fc), targeted (sorafenib), and chemotherapeutic (temozolomide) agents within Intralipid®, which is a biocompatible nanoemulsion with a long history of safe clinical use for total parenteral nutrition. This drug combination has been shown to inhibit tumor growth and angiogenesis with the involvement of the immune system, and a key role is played by ICOS-Fc. The inhibition of tumor growth in subcutaneous melanoma mouse models has been achieved using sub-therapeutic drug doses, which is most likely the result of the nanoemulsion’s targeting properties. If translated to the human setting, this approach should therefore allow therapeutic efficacy to be achieved without increasing the risk of toxic effects.
Nutritional Compounds to Improve Post-Exercise Recovery
Authors: O’Connor Emma , Mündel Toby , Barnes Matthew J.
Published: November 2022

The metabolic and mechanical stresses associated with muscle-fatiguing exercise result in perturbations to bodily tissues that lead to exercise-induced muscle damage (EIMD), a state of fatigue involving oxidative stress and inflammation that is accompanied by muscle weakness, pain and a reduced ability to perform subsequent training sessions or competitions. This review collates evidence from previous research on a wide range of nutritional compounds that have the potential to speed up post-exercise recovery. We show that of the numerous compounds investigated thus far, only two—tart cherry and omega-3 fatty acids—are supported by substantial research evidence. Further studies are required to clarify the potential effects of other compounds presented here, many of which have been used since ancient times to treat conditions associated with inflammation and disease.
Protective Effect and Mechanism of Placenta Extract on Liver
Authors: Shen Liu-Hong , Fan Lei , Zhang Yue , Zhu Ying-Kun , Zong Xiao-Lan , Peng Guang-Neng , Cao Sui-Zhong
Published: November 2022

The placenta contains multiple biologically active substances, which exert antioxidation, anti-inflammatory, immunomodulatory, and delayed aging effects. Its extract can improve hepatic morphology and function: on the one hand, it can reduce liver interstitial collagen deposition, lipogenesis, and inflammatory cell infiltration and improve fibrosis; on the other hand, it can prevent hepatocellular degeneration by scavenging reactive oxygen species (ROS) and inhibiting inflammatory cytokine production, further improve hepatocyte apoptosis and necrosis, and promote hepatocyte regeneration, making it a promising liver-protective agent. Current research on placenta extract (PE) mainly focuses on treating a specific type of liver injury, and there are no systematic reports. Therefore, this review comprehensively summarizes the treatment reports of PE on liver injury and analyzes its mechanism of action.
The Metabolic Role of Ketogenic Diets in Treating Epilepsy
Authors: Imdad Kaleem , Abualait Turki , Kanwal Ammara , AlGhannam Ziyad Tareq , Bashir Shahab , Farrukh Anum , Khattak Sahir Hameed , Albaradie Raidah , Bashir Shahid
Published: November 2022

Epilepsy is a long-term neurological condition that results in recurrent seizures. Approximately 30% of patients with epilepsy have drug-resistant epilepsy (DRE). The ketogenic diet (KD) is considered an effective alternative treatment for epileptic patients. The aim of this study was to identify the metabolic role of the KD in epilepsy. Ketone bodies induce chemical messengers and alterations in neuronal metabolic activities to regulate neuroprotective mechanisms towards oxidative damage to decrease seizure rate. Here, we discuss the role of KD on epilepsy and related metabolic disorders, focusing on its mechanism of action, favorable effects, and limitations. We describe the significant role of the KD in managing epilepsy disorders.
FoMC69 Gene in Fusarium oxysporum f. sp. radicis-lycopersici Is Essential for Pathogenicity by Involving Normal Function of Chlamydospores
Authors: Sasaki Kazunori , Ito Yumi , Hamada Yuki , Dowaki Ayano , Jogaiah Sudisha , Ito Shin-ichi
Published: November 2022

Fusarium oxysporum f. sp. radicis-lycopersici (Forl) causes crown and root rot disease in tomato, effecting severe economic losses. However, research on the pathogenicity genes and infection strategy of Forl is limited compared to that on F. oxysporum f. sp. lycopersici (Fol). In this study, we characterized FoMC69 gene in Forl as a homolog of MC69 required for pathogenicity in rice blast pathogen—Magnaporthe oryzae. Gene expression analysis revealed that FoMC69 expressionin Forl is higher than that in Folin planta. FoMC69-knockout mutant of Forl had significantly reduced root rot symptoms compared to the wild-type strain, and full pathogenicity was restored by complementation. By contrast, ΔFoMC69 mutant of Fol presented the same symptoms as the wild type, suggesting that FoMC69 of Forl, but not of Fol, was essential for full virulence in tomato plants. Morphological differences between the Forl and ΔFoMC69 in the roots were observed by fluorescent labeling using WGA-FITC. Chlamydospores of the ΔFoMC69 mutant of Forlcontinuously increased during infection and were three times higher than that of the wild type at 21 days post-inoculation. These observations suggest that FoMC69 of Forl is required for virulence to tomato plants by involving the normal development and germination of chlamydospores.
Next-Generation Heterocyclic Electrophiles as Small-Molecule Covalent MurA Inhibitors
Authors: Ábrányi-Balogh Péter , Keeley Aaron , Ferenczy György G. , Petri László , Imre Tímea , Grabrijan Katarina , Hrast Martina , Knez Damijan , Ilaš Janez , Gobec Stanislav , Keserű György M.
Published: November 2022

Heterocyclic electrophiles as small covalent fragments showed promising inhibitory activity on the antibacterial target MurA (UDP-N-acetylglucosamine 1-carboxyvinyltransferase, EC:2.5.1.7). Here, we report the second generation of heterocyclic electrophiles: the quaternized analogue of the heterocyclic covalent fragment library with improved reactivity and MurA inhibitory potency. Quantum chemical reaction barrier calculations, GSH (L-glutathione) reactivity assay, and thrombin counter screen were also used to demonstrate and explain the improved reactivity and selectivity of the N-methylated heterocycles and to compare the two generations of heterocyclic electrophiles.
Recent Applications of Contact Lenses for Bacterial Corneal Keratitis Therapeutics: A Review
Authors: Nie Linyan , Li Yuanfeng , Liu Yong , Shi Linqi , Chen Huiyun
Published: November 2022

Corneal keratitis is a common but severe infectious disease; without immediate and efficient treatment, it can lead to vision loss within a few days. With the development of antibiotic resistance, novel approaches have been developed to combat corneal keratitis. Contact lenses were initially developed to correct vision. Although silicon hydrogel-based contact lenses protect the cornea from hypoxic stress from overnight wear, wearing contact lenses was reported as an essential cause of corneal keratitis. With the development of technology, contact lenses are integrated with advanced functions, and functionalized contact lenses are used for killing bacteria and preventing infectious corneal keratitis. In this review, we aim to examine the current applications of contact lenses for anti-corneal keratitis.
Dissecting Complex Traits Using Omics Data: A Review on the Linear Mixed Models and Their Application in GWAS
Authors: Alamin Md. , Sultana Most. Humaira , Lou Xiangyang , Jin Wenfei , Xu Haiming
Published: November 2022

Genome-wide association study (GWAS) is the most popular approach to dissecting complex traits in plants, humans, and animals. Numerous methods and tools have been proposed to discover the causal variants for GWAS data analysis. Among them, linear mixed models (LMMs) are widely used statistical methods for regulating confounding factors, including population structure, resulting in increased computational proficiency and statistical power in GWAS studies. Recently more attention has been paid to pleiotropy, multi-trait, gene–gene interaction, gene–environment interaction, and multi-locus methods with the growing availability of large-scale GWAS data and relevant phenotype samples. In this review, we have demonstrated all possible LMMs-based methods available in the literature for GWAS. We briefly discuss the different LMM methods, software packages, and available open-source applications in GWAS. Then, we include the advantages and weaknesses of the LMMs in GWAS. Finally, we discuss the future perspective and conclusion. The present review paper would be helpful to the researchers for selecting appropriate LMM models and methods quickly for GWAS data analysis and would benefit the scientific society.
Insight into Population Structure and Evolutionary Analysis of the Emerging Tomato Brown Rugose Fruit Virus
Authors: Çelik Ali , Coşkan Sevgi , Morca Ali Ferhan , Santosa Adyatma Irawan , Koolivand Davoud
Published: November 2022

A total of 112 symptomatic tomatoes (Solanum lycopersicum L.) and 83 symptomatic pepper (Capsicum spp.) samples were collected in Ankara, Eskişehir, Bartın, and Zonguldak provinces of Turkey during 2020–2021. Six tomatoes and one pepper sample (3.6%) tested positive for tomato brown rugose fruit virus (ToBRFV, genus Tobamovirus) infection by DAS-ELISA and RT-PCR. ToBRFV-positive tomato and pepper plants were removed from greenhouses as soon as possible, and the greenhouses and tools were disinfected completely. Phylogenetic analysis on the complete CP sequences suggested the clustering of 178 GenBank isolates and 7 novel isolates into three groups. A study using DnaSP software showed very low genetic variation among current global ToBRFV isolates. All four ORFs of the virus genome were under strong negative evolutionary constraints, with a ω value range of 0.0869–0.2066. However, three neutrality tests indicated that most populations of the newly identified ToBRFV are currently expanding by assigning statistically significant negative values to them. The very low FST values (0.25 or less) obtained by all comparisons of the isolates from Europe, the Middle East, China, and America concluded that there is no clear genetic separation among currently known isolates from different geographic origins. The divergence time of ToBRFV was estimated to be in the middle of the course of the evolution of 11 tested tobamoviruses. The time to the most recent common ancestors (TMRCAs) of ToBRFV were calculated to be 0.8 and 1.87 with the genetically closest members of Tobamovirus. The results of this study could improve our understanding on the population structure of the emerging ToBRFV.
Evaluating Germplasm of Cultivated Oat Species from the VIR Collection under the Russian Northwest Conditions
Authors: Popov Vitaliy S. , Khoreva Valentina I. , Konarev Alexei V. , Shelenga Tatyana V. , Blinova Elena V. , Malyshev Leonid L. , Loskutov Igor G.
Published: November 2022

Oat is one of the most widespread and important cereal crops in the global agricultural production. Searching for new high-yielding and nutritious forms continues to be relevant, especially under the global trend of climate change, when most local oat cultivars may become economically inefficient. Spring oat accessions from VIR collection served as the material for this study; their origin is diverse, as they came from 11 countries. The basic nutritional value (the content of protein, oil, starch, and β-glucans) and characters important for breeding (plant height, panicle length, number of spikelets, number of grains per panicle, 1000 grain weight, and grain yield) were analyzed in 49 accessions of the cultivated covered oat species: Avena sativa L., A. strigosa Schreb., A. abyssinica Hochst., and A. byzantina Coch., grown under the conditions of the Russian Northwest (Leningrad Province) for two years. Variability parameters, interspecific and intervarietal differences, and the effect of weather conditions were assessed. Sources of useful agronomic traits were identified; they can be used to expand the range of the source material for the development of new high-yielding and highly nutritious oat cultivars adapted to local cultivation conditions. It is demonstrated that the VIR collection has a great potential for contemporary food and feed production and for the breeding of new oat cultivars for various purposes. Thus, the contribution of Nikolai Vavilov to the plant genetic resources investigation for the benefit of humanity is invaluable.
A Comparative Analysis of Chemical, Plasma and In Situ Modification of Graphene Nanoplateletes for Improved Performance of Fused Filament Fabricated Thermoplastic Polyurethane Composites Parts
Authors: Zhang Xiaojie , Xiao Jianhua , Kim Jinkuk , Cao Lan
Published: November 2022

The limited number of materials and mechanical weakness of fused deposition modeling (FDM) parts are deficiencies of FDM technology. The preparation of polymer composites parts with suitable filler is a promising method to improve the properties of the 3D printed parts. However, the agglomerate of filler makes its difficult disperse in the matrix. In this work, graphene nanoplatelets (GnPs) were surface modified with chemical, low-temperature plasma and in situ methods, in order to apply them as fillers for thermoplastic polyurethane (TPU). Following its modification, the surface chemical composition of GnPs was analyzed. Three wt% of surface-modified GnPs were incorporated into TPU to produce FDM filaments using a melting compounding process. Their effects on rheology properties and electrical conductivity on TPU/GnPs composites, as well as the dimensional accuracy and mechanical properties of FDM parts, are compared. The images of sample facture surfaces were examined by scanning electron microscope (SEM) to determine the dispersion of GnPs. Results indicate that chemical treatment of GnPs with zwitterionic surfactant is a good candidate to significantly enhance TPU filaments, when considering the FDM parts demonstrated the highest mechanical properties and lowest dimensional accuracy.
Response Surface Methodology (RSM) Powered Formulation Development, Optimization and Evaluation of Thiolated Based Mucoadhesive Nanocrystals for Local Delivery of Simvastatin
Authors: Bakhaidar Rana B. , Naveen Nimbagal Raghavendra , Basim Pratap , Murshid Samar S. , Kurakula Mallesh , Alamoudi Abdulmohsin J. , Bukhary Deena M. , Jali Abdulmajeed M. , Majrashi Mohammed A. , Alshehri Sameer , Alissa Mohammed , Ahmed Rayan A.
Published: November 2022

In oral administration systems, mucoadhesive polymers are crucial for drug localization and target-specific activities. The current work focuses on the application of thiolated xanthan gum (TXG) to develop and characterize a novel mucoadhesive nanocrystal (NC) system of simvastatin (SIM). Preparation of SIM-NC was optimized using response surface methodology (RSM) coupled with statistical applications. The concentration of Pluronic F-127 and vacuum pressure were optimized by central composite design. Based on this desirable approach, the prerequisites of the optimum formulation can be achieved by a formulation having 92.568 mg of F-127 and 77.85 mbar vacuum pressure to result in EE of 88.8747% and PS of 0.137.835 nm. An optimized formulation was prepared with the above conditions along with xanthan gum (XG) and TXG and various parameters were evaluated. A formulation containing TXG showed 98.25% of SIM at the end of 96 h. Regarding the mucoadhesion potential evaluated by measuring zeta potential, TXG-SIM-NC shoed the maximum zeta potential of 16,455.8 ± 869 mV at the end of 6 h. The cell viability percentage of TXG-SIM-NC (52.54 ± 3.4% with concentration of 50 µg/mL) was less than the plain SIM, with XG-SIM-NC showing the highest cytotoxicity on HSC-3 cells. In vivo pharmacokinetic studies confirm the enhanced bioavailability of formulated mucoadhesive systems of SIM-NC, with TXG-SIM-NC exhibiting the maximum.
Formation and Characterization of Oregano Essential Oil Nanocapsules Applied onto Polyester Textile
Authors: Salinas Carla , Lis Manuel J. , Coderch Luisa , Martí Meritxell
Published: November 2022

Oregano essential oil was encapsulated in poly-ϵ-caprolactone nanoparticles by a nanoprecipitation method using glycerin as a moisturizer. Nanocapsule characterization was performed by measuring the particle size, colloidal stability and encapsulation efficiency using dynamic light scattering, UV–Vis spectrophotometry and scanning electron microscopy (SEM). The nanoparticles had a mean particle size of 235 nm with a monomodal distribution. In addition, a low polydispersity index was obtained, as well as a negative zeta potential of −36.3 mV and an encapsulation efficiency of 75.54%. Nanocapsules were applied to polyester textiles through bath exhaustion and foulard processing. Citric acid and a resin were applied as crosslinking agents to improve the nanocapsules’ adhesion to the fabric. The adsorption, desorption, moisture content and essential oil extraction were evaluated to determine the affinity between the nanocapsules and the polyester. The adsorption was higher when the citric acid and the resin were applied. When standard oregano nanocapsules were used, almost all of the impregnated nanoparticles were removed when washed with water. The moisture content was evaluated for treated and non-treated textiles. There was a significant increase in the moisture content of the treated polyester compared to the non-treated polyester, which indicates that the polyester hydrophilicity increased with an important absorption of the essential oil nanocapsules; this can improve fabric comfort and probably promote antibacterial properties.
Magnetic Extraction of Weathered Tire Wear Particles and Polyethylene Microplastics
Authors: Budhiraja Vaibhav , Mušič Branka , Krzan Andrej
Published: November 2022

Magnetic extraction offers a rapid and low-cost solution to microplastic (MP) separation, in which we magnetize the hydrophobic surface of MPs to separate them from complex environmental matrices using magnets. We synthesized a hydrophobic Fe-silane based nanocomposite (Fe@SiO2/MDOS) to separate MPs from freshwater. Pristine and weathered, polyethylene (PE) and tire wear particles (TWP) of different sizes were used in the study. The weathering of MPs was performed in an accelerated weathering chamber according to ISO 4892-2:2013 standards that mimic natural weathering conditions. The chemical properties and morphology of the Fe@SiO2/MDOS, PE and TWP were confirmed by Fourier transform infrared spectroscopy and Scanning electron microscopy, respectively. The thermal properties of PE and TWP were evaluated by Thermogravimetric analysis. Using 1.00 mg of Fe@SiO2/MDOS nanocomposite, 2.00 mg of pristine and weathered PE were extracted from freshwater; whereas, using the same amount of the nanocomposite, 7.92 mg of pristine TWP and 6.87 mg of weathered TWP were extracted. The retrieval of weathered TWP was 13% less than that of pristine TWP, which can be attributed to the increasing hydrophilicity of weathered TWP. The results reveal that the effectiveness of the magnetic separation technique varies among different polymer types and their sizes; the weathering of MPs also influences the magnetic separation efficiency.
Territories of Contention: The Importance of Project Location in Mining-Related Disputes in Finland from the Geosystem Services Perspective
Authors: Eerola Toni
Published: November 2022

Geosystem services produced by geological processes are the abiotic equivalents of ecosystem services. Geosystems also contribute to satisfying human needs and produce welfare in the form of, e.g., mineral deposits, landscapes for recreation and tourism, and habitats for rare species that require protection. Geosystems are inherently linked to ecosystems, which causes overlap between provided services. This overlap may in turn cause conflicts over land-use needs and interests. Such controversies can be manifested as mining and mineral exploration disputes (MMEDs). Six MMEDs from Finland were selected for a closer examination. The MMEDs are described and spatially analyzed from the geosystem services perspective. The main causes for the examined MMEDs are land-use issues, i.e., the location of a project in a sensitive context (a protected area (PA), tourism destination, reindeer herding area, or lake area with vacation homes), and/or association with uranium. There have been attempts to block some of the projects through land-use planning by expanding PAs or excluding mining from the municipality. Conversely, one of the projects is an example of the safeguarding of mineral deposits by province-level land-use planning. A more comprehensive consideration of geosystem services by land-use planning may help to accommodate and reconciliate diverse interests and alleviate disputes.
Monitoring Short-Term Morphobathymetric Change of Nearshore Seafloor Using Drone-Based Multispectral Imagery
Authors: Alevizos Evangelos , Alexakis Dimitrios D.
Published: November 2022

Short-term changes in shallow bathymetry affect the coastal zone, and therefore their monitoring is an essential task in coastal planning projects. This study provides a novel approach for monitoring shallow bathymetry changes based on drone multispectral imagery. Particularly, we apply a shallow water inversion algorithm on two composite multispectral datasets, being acquired five months apart in a small Mediterranean sandy embayment (Chania, Greece). Initially, we perform radiometric corrections using proprietary software, and following that we combine the bands from standard and multispectral cameras, resulting in a six-band composite image suitable for applying the shallow water inversion algorithm. Bathymetry inversion results showed good correlation and low errors (<0.3 m) with sonar measurements collected with an uncrewed surface vehicle (USV). Bathymetry maps and true-color orthomosaics assist in identifying morphobathymetric features representing crescentic bars with rip channel systems. The temporal bathymetry and true-color data reveal important erosional and depositional patterns, which were developed under the impact of winter storms. Furthermore, bathymetric profiles show that the crescentic bar appears to migrate across and along-shore over the 5-months period. Drone-based multispectral imagery proves to be an important and cost-effective tool for shallow seafloor mapping and monitoring when it is combined with shallow water analytical models.
Reverse Thinking: The Logical System Research Method of Urban Thermal Safety Pattern Construction, Evaluation, and Optimization
Authors: Hu Chunguang , Li He
Published: November 2022

The acceleration of urbanization has significantly impacted the changing regional thermal environment, leading to a series of ecological and environment-related problems. A scientific evaluation of the urban thermal security pattern (TSPurban) strongly benefits the planning and layout of sustainable development and the construction of comfortable human settlements. To analyze the characteristics of the TSPurban under cross-regional differences and provide targeted solutions to mitigate the urban heat island effect in later stages, the logical system research framework of the TSPurban based on the “construction–evaluation–optimization” model was explored using reverse thinking. This study selected the Wuhan metropolitan area in China as the research object. First, a morphological spatial pattern analysis (MSPA) model was used to extract the top 30 core heat island patches, and Conefor 2.6 software was used for connection analysis to evaluate their importance. Second, based on the characteristics of various land cover types, the friction (cost) map of surface urban heat island (SUHI) diffusion was simulated. The spatial attributes of the heat island resistance surface were examined using a standard deviation ellipse and hot spot analysis. Finally, this paper used circuit theory to find 56 low-cost heat island links (corridors) and circuit scape software to find widely distributed vital nodes. The optimization of the TSPurban network was then investigated using a reverse thinking process. Heat island patches, corridors, and vital nodes are among the crucial components of the TSPurban. By obstructing corridor links and disturbing important nodes, it is possible to appropriately and proficiently reduce the TSPurban network’s connection efficiency and stability, which will have a positive influence on regional climate mitigation and the heat island effect.
Design and Fabrication of Interdigital Supercapacitors as Force/Acceleration Sensors
Authors: Huang Jue , Dai Keren , Yin Yajiang , Chen Zhaorong , You Zheng , Wang Xiaofeng
Published: November 2022

The integrated device for energy supply and sensing (IDESS) is a potential candidate for relieving the energy and space burdens caused by the rising integration degrees of microsystems. In this article, we propose a force sensor based on an interdigital supercapacitor (IDTSC). The capacitance and internal resistance of the IDTSC change under external loads, resulting in a transient current fluctuation at a constant bias voltage, which can be used to sense external force/acceleration. The IDTSC showed a specific energy and specific power of 4.16 Wh/kg and 22.26 W/kg (at 0.1 A/g), respectively, which could maintain an essential energy supply. According to the simulation analysis, the designed IDTSC’s current response exhibited good linearity with the external force. In addition, benefiting from its light weight and the applied gel electrolytes, the IDTSC showed good high-g impact sensing performance (from 9.9 × 103× g to 3.2 × 104× g). This work demonstrated the feasibility of realizing an integrated energy supply and force-sensing device by empowering energy storage devices with sensing capabilities.
Detection of Green Asparagus Using Improved Mask R-CNN for Automatic Harvesting
Authors: Liu Xiangpeng , Wang Danning , Li Yani , Guan Xiqiang , Qin Chengjin
Published: November 2022

Advancements in deep learning and computer vision have led to the discovery of numerous effective solutions to challenging problems in the field of agricultural automation. With the aim to improve the detection precision in the autonomous harvesting process of green asparagus, in this article, we proposed the DA-Mask RCNN model, which utilizes the depth information in the region proposal network. Firstly, the deep residual network and feature pyramid network were combined to form the backbone network. Secondly, the DA-Mask RCNN model added a depth filter to aid the softmax function in anchor classification. Afterwards, the region proposals were further processed by the detection head unit. The training and test images were mainly acquired from different regions in the basin of the Yangtze River. During the capturing process, various weather and illumination conditions were taken into account, including sunny weather, sunny but overshadowed conditions, cloudy weather, and daytime greenhouse conditions as well as nighttime greenhouse conditions. Performance experiments, comparison experiments, and ablation experiments were carried out using the five constructed datasets to verify the effectiveness of the proposed model. Precision, recall, and F1-score values were applied to evaluate the performances of different approaches. The overall experimental results demonstrate that the balance of the precision and speed of the proposed DA-Mask RCNN model outperform those of existing algorithms.
Design of a High Sensitivity Pirani Gauge Based on Vanadium Oxide Film for High Vacuum Measurement
Authors: Guo Song , Feng Liuhaodong , Chen Shuo , Ji Yucheng , Peng Xinlin , Xu Yang , Yin Yong , Wang Shinan
Published: November 2022

We have designed a hot-plate-type micro-Pirani vacuum gauge with a simple structure and compatibility with conventional semiconductor fabrication processes. In the Pirani gauge, we used a vanadium oxide (VOx) membrane as the thermosensitive component, taking advantage of the high temperature coefficient of resistance (TCR) of VOx. The TCR value of VOx is −2%K−1∼−3%K−1, an order of magnitude higher than those of other thermal-sensitive materials, such as platinum and titanium (0.3%K−1∼0.4%K−1). On one hand, we used the high TCR of VOx to increase the Pirani sensitivity. On the other hand, we optimized the floating structure to decrease the thermal conductivity so that the detecting range of the Pirani gauge was extended on the low-pressure end. We carried out simulation experiments on the thermal zone of the Pirani gauge, the width of the cantilever beam, the material and thickness of the supporting layer, the thickness of the thermal layer (VOx), the depth of the cavity, and the shape and size. Finally, we decided on the basic size of the Pirani gauge. The prepared Pirani gauge has a thermal sensitive area of 130 × 130 μm2, with a cantilever width of 13 μm, cavity depth of 5 μm, supporting layer thickness of 300 nm, and VOx layer thickness of 110 nm. It has a dynamic range of 10−1~104 Pa and a sensitivity of 1.23 V/lgPa. The VOx Pirani was designed using a structure and fabrication process compatible with a VOx-based uncooled infrared microbolometer so that it can be integrated by wafer level. This work contains only our MEMS Pirani gauge device design, preparation process design, and readout circuit design, while the characterization and relevant experimental results will be reported in the future.
A Novel Preanalytical Strategy Enabling Application of a Colorimetric Nanoaptasensor for On-Site Detection of AFB1 in Cattle Feed
Authors: Contreras-Trigo Braulio , Díaz-García Víctor , Oyarzún Patricio
Published: November 2022

Aflatoxin contamination of cattle feed is responsible for serious adverse effects on animal and human health. A number of approaches have been reported to determine aflatoxin B1 (AFB1) in a variety of feed samples using aptasensors. However, rapid analysis of AFB1 in these matrices remains to be addressed in light of the complexity of the preanalytical process. Herein we describe an optimization on the preanalytical stage to minimize the sample processing steps required to perform semi-quantitative colorimetric detection of AFB1 in cattle feed using a gold nanoparticle-based aptasensor (nano-aptasensor). The optical behavior of the nano-aptasensor was characterized in different organics solvents, with acetonitrile showing the least interference on the activity of the nan-aptasensor. This solvent was selected as the extractant agent for AFB1-containing feed, allowing for the first time, direct colorimetric detection from the crude extract (detection limit of 5 µg/kg). Overall, these results lend support to the application of this technology for the on-site detection of AFB1 in the dairy sector.
Feature Selection for Continuous within- and Cross-User EEG-Based Emotion Recognition
Authors: Bendrich Nicole , Kumar Pradeep , Scheme Erik
Published: November 2022

The monitoring of emotional state is important in the prevention and management of mental health problems and is increasingly being used to support affective computing. As such, researchers are exploring various modalities from which emotion can be inferred, such as through facial images or via electroencephalography (EEG) signals. Current research commonly investigates the performance of machine-learning-based emotion recognition systems by exposing users to stimuli that are assumed to elicit a single unchanging emotional response. Moreover, in order to demonstrate better results, many models are tested in evaluation frameworks that do not reflect realistic real-world implementations. Consequently, in this paper, we explore the design of EEG-based emotion recognition systems using longer, variable stimuli using the publicly available AMIGOS dataset. Feature engineering and selection results are evaluated across four different cross-validation frameworks, including versions of leave-one-movie-out (testing with a known user, but a previously unseen movie), leave-one-person-out (testing with a known movie, but a previously unseen person), and leave-one-person-and-movie-out (testing on both a new user and new movie). Results of feature selection lead to a 13% absolute improvement over comparable previously reported studies, and demonstrate the importance of evaluation framework on the design and performance of EEG-based emotion recognition systems.
On Transient Queue-Size Distribution in a Model of WSN Node with Threshold-Type Power-Saving Algorithm
Authors: Kempa Wojciech M. , Kurzyk Dariusz
Published: November 2022

This article proposes a queueing model of the operation of a wireless sensor network node, in which a threshold strategy for starting the node after a period of no transmission is used. In this model, transmission of packets is resumed when the number of packets in the accumulation buffer reaches a predefined level. In the literature, most of the results for models with limited access to the service station are obtained in equilibrium. In this paper, a formula for the Laplace transform of the transient queue-size distribution is obtained and written using the key input parameters of the system. The analytical apparatus uses the concept of the embedded Markov chain, the formula for total probability, renewal theory and some supporting algebraic results. Numerical examples are attached as well.
Agenda-Setting Dynamics during COVID-19: Who Leads and Who Follows?
Authors: Šķestere Lāsma , Darģis Roberts
Published: November 2022

The outbreak of the coronavirus (COVID-19) has altered the way news media and social media set their agendas. The growth of social media raises questions about its potential power to set the media agenda. We gathered social media posts and online news site articles to examine agenda-setting dynamics, aiming to explore causal relationship between news media and social media. We used a computer-assisted text analysis to discover the main topics of discussion at the first wave of the pandemic in Latvia. The results revealed that (1) statistics about the pandemic, as well as prevention and control measures were the main topics on social media and in online news sites, and that (2) vector autoregression models provide more empirical support for the influence of online news sites on social media than reverse.
Redefining the Use of Vinification Waste By-Products in Broiler Diets
Authors: Giamouri Elisavet , Mavrommatis Alexandros , Simitzis Panagiotis E. , Mitsiopoulou Christina , Haroutounian Serkos A. , Koutinas Apostolis , Pappas Athanasios C. , Tsiplakou Eleni
Published: November 2022

In this study, the use of vinification by-products in broiler diets, as a sustainable and promising way of exploiting them, was examined. In particular, the potential use of ground grape pomace (GGP), wine lees extract (WYC) and grape stem extract (PE) in broiler diets was examined. Growth performance parameters, the weight of selected internal organs, meat quality traits, fatty acid profiles of breast meat and selected haematological parameters were determined. Two hundred and forty one-day-old broilers were assigned to four treatments with four replicate pens of fifteen broilers. There was one control treatment (CON), fed a basal diet, and the GGP, WYC and PE treatments, fed a basal diet supplemented with 25 g/kg GGP, 2 g/kg WYC and 1 g starch including 100 mg pure stem extract/kg PE, respectively. The duration of the experiment was 42 days. The average body weight gain during the starter, grower and finisher stages did not differ among treatments. Similarly, the feed intake, FCR and carcass yield did not show a significant difference. The weight of the internal organs was also similar among treatments. Some positive differences were observed in colour traits of meat and in haematological parameters. In the GGP group, saturated (SFAs) and unsaturated fatty acids (USFAs) were lower and higher, respectively, compared to the CON, WYC and PE groups. Vinification by-products seem to be a promising feed additive in broiler diets providing a sustainable approach to grape waste management without affecting broiler performance.
An Overview of Thermal Treatment Emissions with a Particular Focus on CO2 Parameter
Authors: Panepinto Deborah , Ravina Marco , Zanetti Mariachiara
Published: November 2022

Waste-to-energy (WtE) technologies can offer sustainable solutions for waste that cannot be further reused or recycled, such as the part of municipal solid waste (MSW) that is not suitable for recycling processes. The two main (most widely used) thermal treatment technologies that can be applied to MSW are direct combustion in an incineration plant and gasification. This paper examines in particular the direct combustion in incineration plants, explaining the main process, the main technologies applied, and the resulting environmental aspects. Moreover, this work focuses on analyzing flue gas emissions from thermal treatment in order to better understand the impacts of these kinds of processes. A particular focus on the CO2 parameter is performed. CO2 is a persistent atmospheric gas, and it is one of the greenhouse gases (GHGs) potentially responsible for the climate change phenomenon. In this sense, specific indexes (tCO2/tMSW and tCO2/MWh) are elaborated considering the thermal treatment plants present in six Italian regions. The main aim of this review paper is to try to fill the gap that still exists regarding the emissions environmental compatibility coming from these type of plants, the evaluation of the amount of CO2 emitted, and the possible reduction of the CO2 parameter. One of the main outcome obtained is in fact the evaluation of the amount of CO2 coming from these kinds of plants and some indications about the technological possibilities of reducing this amount.
Logistics Center Location-Inventory-Routing Problem Optimization: A Systematic Review Using PRISMA Method
Authors: Liu Lihua , Lee Lai Soon , Seow Hsin-Vonn , Chen Chuei Yee
Published: November 2022

A traditional logistics decision model mainly studies the location decision of logistics distribution centers, storage inventory management, vehicle scheduling, and transportation routes. The logistics location-inventory-routing problem (LIRP) is an integrated optimization of the three problems—a comprehensive optimization problem for the whole logistics system. This review paper uses the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) method to review the literature on LIRP systematically. A total of 112 LIRP-related studies published between 2010 and 2021 are reviewed and classified based on 10 abstract and citation databases. The classification includes four aspects: problem characteristics, demand data types, model-based solutions, and application fields. From this systematic review, a few observations are recorded. First, the most popular problems among researchers are the multi-period multi-product problem, the multi-echelon single-link problem, and the multi-depot multi-retailer problem. Based on the objective function, the minimization of total supply chain cost is the primary concern of the LIRP literature. Researchers also favor other problem characteristics such as multi-objective programming, inventory control replenishment policy, and a homogeneous fleet of vehicles. We found that stochastic data are a common factor in an uncertain environment and have broad coverage. When dealing with the LIRP, heuristic and metaheuristic algorithms are the most widely used solution methodologies in the literature. In the application field of LIRP, the perishable products logistics network is mentioned in most applications. Finally, we discuss and emphasize the challenges of and recommendations for future work. This paper provides a systematic review of the literature on LIRP based on the PRISMA method, which contributes vital support and valuable information for researchers interested in LIRP.
Elevation-Dependent Fluctuations of the Soil Properties in a Subtropical Forest of Central China
Authors: Ju Fanfan , Chen Liuzhu , Zheng Jiejun , Chen Zhanqiang , Wang Xiaoli , Xia Xinxing
Published: November 2022

Understanding the contents and stoichiometry of carbon (C), nitrogen (N), and phosphorus (P) is vital to evaluate the function and processes of a forest ecosystem. Overall, 18 sites in Shennongjia Forest from an altitude from 800 to 3000 m were selected to collect litterfall, humus, and soil (0–20 and 20–40 cm) samples in May, August, and December. The spatio-temporal distribution of C, N, and P contents and their stoichiometry were quantified, and the underlying driving factors were analyzed. Results revealed total organic carbon (TOC) and total nitrogen (TN) contents decreased from the topsoil to the deeper soil, while total phosphorus (TP) contents in the soil changed slightly with depth. Controlled by various sources and decomposition degrees, the ratios of C:P, C:N, and N:P decreased from litterfall to humus, further increased in topsoil, and decreased again in deeper soil. Considering the average values of all sites, only TN in litterfall and humus dissolved organic carbon (DOC) in soil, and C:N in litterfall exhibited a significant seasonal variation. With increasing altitude, the contents of TOC, TN, and TP significantly increased in soil, particularly in August, but fluctuated in litterfall and humus. This positive relationship in soil was remarkable for TOC and TN compared with TP. Pearson’s correlation and redundancy analysis indicated driving factors exhibited a more noticeable influence on the contents of TOC, TN, and TP in soil than those in litterfall and humus. Moisture content, vegetation pattern, bulk density, total Mn (tMn), total Fe (tFe), and clay content observably influenced the contents of TOC, TN, and TP in the soil, and thus affected its stoichiometry. This investigation provided a comparable dataset on the contents of C, N, and P and their patterns of stoichiometry, which are helpful to optimize forest management and ecosystems.