bluuebunny/crossref_metadata_2025 huggingface dataset:
Implementation of Digital Marketing Strategies to Increase Sales during the Covid-19 Pandemic
Authors: Effendy Aidil Amin , Mas’adi Mahnun , Murtiyoko Heri
Published: July 2022

This study aims to determine the implementation of digital marketing strategies and how effective digital marketing strategies are to increase sales during the Covid-19 pandemic (Studies on SMEs in South Tangerang City). This research method uses qualitative methods by gaining a deep understanding of data collection techniques through observation, documentation, and interviews, using the semi-structured interview method based on predetermined interview guides for several informants to MSME actors in the city of South Tangerang. The analysis results from the interviews show that the most effective and efficient marketing is done digitally / online, primarily through digital/online marketing. According to MSME Players in South Tangerang, they prefer to market their products through Facebook, Instagram, and Online Shop, then WhatsApp, and there are also MSME players marketing them through GoFood and GrabFood. Furthermore, the results of interviews with other informants regarding the determinants of MSMEs increase their sales during the Covid-19 Pandemic, namely promotion factors, with digital/online marketing and competitive price factors.
Analysis of the Implementation of the 2015-2017 National Identity Card Administration Service Policy (KTP-El) by the District Government of Bima
Authors: Yuliati Dewi
Published: July 2022

The expected implication of the decentralization and regional autonomy policies is strengthening local government functions in providing excellent public services. According to Law Number 25 of 2009, public services include serving the needs of the population for public goods, public services, and administrative services. In terms of population administration, the government tries to serve the public professionally, meets information technology standards, is dynamic, orderly and non-discriminatory. Population administration includes services for the issuance of electronic Identity Cards (KTP-el). The thee-KTP Issuance Policy is stated in Law Number 24 of 2013. The implementors of the thee-KTP policy are all Provincial and Regency/City Governments. The Implementing Agency is the Population and Civil Registry Office of the Regency/City government. This research was conducted with a descriptive analysis method. The main problem of this research is how the process of implementing the e-KTP policy in Bima Regency and the analysis of the variation in the population's response to the thee-KTP issuance service they receive. The government has taken various steps in order to realize the modernization of population administration. However, in the implementation of the policy for issuing electronic ID cards (KTP-el), there are still various challenges. Population satisfaction has not been met. Service implementing agencies, namely the Population Service Office and Bima Regency, still face various challenges in meeting the expectations of the population. It is concluded that the implementation of the e-KTP issuance policy is basically complex and not easy.
The Influence of Mood and Price Shop
Authors: Wulansari Retno
Published: July 2022

This study aims to determine the effect of shop atmosphere and price on consumer buying interest in Hypermarket in Kota Tangerang Selatan. The method used is explanatory research with analysis techniques using statistical analysis with regression testing, correlation, determination, and hypothesis testing. The results of this study, the shop atmosphere has a significant effect on buying interest by 39.2%, hypothesis testing obtained t count> t table or (6,719> 1,994). Price has a significant effect on buying interest by 54.6%; hypothesis testing is obtained t count> t table or (9.173> 1.994). Shop atmosphere and prices simultaneously have a significant effect on buying interest with the regression equation Y = 8.676 + 0.254X1 + 0.543X2, and the contribution of the effect is 57.9%, hypothesis testing is obtained F count> F table or (47.424> 2.740).
Influence of Operational Costs on Operating Revenue and Loan to Deposit Ratio against Return on Asset at PT. Bank Negara Indonesia, Tbk Year 2009-2019
Authors: Jati Waluyo
Published: July 2022

This study aims to determine the operational costs operating income (BOPO) and Loan to Deposit Ratio (LDR) on Return on Assets at PT. Bank Negara Indonesia, Tbk. The method used is explanatory research. The analysis technique uses statistical analysis with regression testing, correlation, determination and hypothesis testing. The results of this study Operational Costs Operational Income (BOPO) has a significant effect on Return on Assets by 40.7%, hypothesis testing is obtained t count> t table or (2.346> 2.262). Loan to Deposit Ratio (LDR) has a significant effect on Return on Assets of 53.9%; hypothesis testing is obtained t count> t table or (-3.056> -2.262). Operating Costs Operational Income (BOPO) and Loan to Deposit Ratio (LDR) simultaneously have a significant effect on Return on Assets, the regression equation Y = 1.565 + 0.205X1 - 0.089X2 and a determination value of 59.9%, hypothesis testing obtained the F value calculated > F table or (5,224> 4,350).
The Effect of Discipline on Employee Productivity at PT. Cipta Selaras in Pamulang
Authors: Indarto Bagus Kuncoro , Rusilowati Umi
Published: July 2022

This study aims to determine the effect of discipline on employee productivity at PT. Cipta Selaras in Pamulang. The method used is explanatory research with a sample of 56 respondents. The analysis technique uses statistical analysis with regression, correlation, determination and hypothesis testing. The results of this study of the discipline variable obtained an average score of 3.748 with suitable criteria. Employee productivity variable obtained an average score of 3.868 with suitable criteria. Discipline has a positive and significant effect on employee productivity with a regression equation value of Y = 14.665 + 0.641X, and a correlation coefficient value of 0.766 has a strong relationship with a determination value of 58.7%. Hypothesis testing obtained a significance of 0.000 < 0.05. abstracts are typed in single spaces.
The Effect of Work Environment on Employee Performance at PT. Sinar Citra Abadi in Pamulang
Authors: Novendri Indra , Budiman Surya
Published: July 2022

This study aims to determine the effect of the work environment on employee performance at P.T. Sinar Citra Abadi in Pamulang. The method used is explanatory research with a sample of 55 respondents. The analysis technique uses statistical analysis with regression, correlation, determination and hypothesis testing. This study of the work environment variable obtained an average score of 5.616 with suitable criteria. Employee performance variable obtained an average score of 5.729 with suitable criteria. The work environment has a positive and significant effect on employee performance with the regression equation Y = 20.320 + 0.658X, and the correlation coefficient value of 0.750 or has a strong relationship with a determination value of 56.3%. Hypothesis testing obtained a significance of 0.000 <0.05.
The Effect of Remuneration on Employee Performance at PT. BNI Syariah Bintaro Branch Office
Authors: Nugroho Andy , Sutoro Moh.
Published: July 2022

This study aims to determine the effect of remuneration on employee performance at PT. BNI Syariah Bintaro Branch Office. The method used is explanatory research with a sample of 45 respondents. The analysis technique uses statistical analysis with regression, correlation, determination, and hypothesis testing. The results of this study Remuneration variable obtained an average score of 3.773 with good criteria. Employee performance variable obtained an average score of 3.898 with good criteria. Remuneration has a positive and significant effect on employee performance with the regression equation Y = 10.035 + 0.826X, and the correlation coefficient value is 0.826 or has a strong relationship with a determination value of 68.2%. Hypothesis test obtained sig. 0.000 < 0.05
The Role of Support From Supervisors and Co-Workers on Financial Service Marketing Agents’ Performance in The Aspects of Work Motivation and Satisfaction
Authors: Abdullatif Rafiqah Saputri , Anindita Rina
Published: July 2022

The barrier between employees, or the barrier between employees and supervisors in the marketing agency context has been a serious issue that causes detrimental consequences, such as demotivation, degrading productivity, and reduced work performance among employees. The importance of the supervisor and co-worker support has now become a crucial topic, as it is proven to be advantageous for organizations. This research was conducted using questionnaires back in December 2019 until January 2020, in which the respondents consisted of 230 marketing agencies of financial services in DKI Jakarta. The data was analyzed using Structural Equation Model (SEM).  There are some significant results in this research. Firstly, there is an association between supervisor support and employee motivation. Secondly, there is an association between co-worker support and work motivation. Next, there is an association between co-worker support and job satisfaction. Fourth, there is an association between work motivation and job satisfaction. Moreover, there is an association between work motivation and employee performance. Lastly, there is an association between job satisfaction and employee performance. However, there is no significant association between supervisor support and job satisfaction.
Developing Management Application for State Properties at State Islamic Institute of Palopo
Authors: Asdiany Diah , Putrasyam Khaidir Mubarak , Ilham Dodi
Published: July 2022

This study aims to: (1) Obtain an information system for monitoring the use of State-Owned Goods inventory at IAIN Palopo (2) The system built can be a solution in managing and monitoring the use of State-Owned Goods inventory. The type of research used is Research and Development (R & R&D), with the Waterfall Model development model consisting of five stages, namely Requirement Analysis and Definition, System and Software Design, Implementation and Unit Testing, Integration and System Testing, and Operation maintenance. The results of this study are in the form of an information system that can facilitate data management and monitoring the use of State Property at IAIN Palopo.
Financial Support on SMEs Impact of the Covid 19 Pandemic
Authors: Gunartin Gunartin , Pratikto Heri , Sopiah Sopiah
Published: July 2022

The purpose of this literature review is to reveal the role of financial support for MSMEs as a result of the Covid-19 pandemic. This paper is sourced from secondary data with a review approach from the previous article. Several articles from various countries have produced that finance has a very important role in the sustainability of MSMEs. Restrictions on community activities as an effort to prevent exposure to Covid-19 impact the decline of the economy in various countries, which results in a decline in the performance of MSMEs. The decline in people's purchasing power impacts the decline in aggregate demand for goods and services, causing a decrease in income for MSME actors. Under these conditions, MSMEs need financial support from various parties for their business continuity. With financial support (capital strengthening or financing), it is hoped that MSME actors can continue their business amidst the economic crisis due to the Covid-19 pandemic. It can be concluded that financial support provides strength for MSMEs to cover operational costs and survive in conditions of economic crisis. Financial support can be in the form of disbursing stimulus funds in cash or credit, tax reductions, credit interest relief, deferral of tax payments, reduced rental fees, exemption from administrative costs to open access to financing or capital. Apart from financial support, it is also carried out to accelerate the digitization of MSMEs to open access to market expansion. Also, the women's business empowerment program (Puspa) from the East Java provincial government to increase family income.
Strategic Policy for Acceleration and Optimization of Border Area Development
Authors: Setyasih Endang Try , Purwanto Bambang Heru , Jusuf Sp Eddy
Published: July 2022

Most of the regions in the border are less developed due to the development paradigm in the past, more emphasizing the security approach rather than the security approach and inward-looking instead of outward-looking. In essence, the government has been striving to expedite border region development, but the border region is still less developed and marginalized. The roadmap of authority in border areas yet to be delimited between Government and Local Government, lack of synergic coordination, limited budget, the hardship of topographical features, isolated, remote, and inaccessible areas are the major problems. Therefore, to rapidly foster and prosper millions living in border regions, the government should formulate strategic and stringent policies.
Service Quality Improvement Strategy in Library Management at Tadulako University
Authors: Andarbeni Prita , Salamah Ummu , Satibi Iwan
Published: July 2022

This study wants to examine how the Tadulako University library can improve its services to users through the development of a strategic plan. It aims to: (1) study and analyzes the quality of services using Tangible dimensions measurement methods (physical appearance), Reliability, Responsiveness, Assurance, and Empathy in the management of services at the University Tadulako; and (2) find strategies to improve the quality of services in the Tadulako University library management. The quantitative and qualitative (mix method) approaches are used in this study to obtain comprehensive, valid, reliable, and objective data. The service quality model uses the Servqual by Zeitham et al. In addition, a descriptive analysis approach is used to provide a description of the research subjects based on variable data. The number of samples is 397 people determined using the Slovin formula, with proportional sampling techniques. The data analysis technique used is the Mathew B. Miles and A. Michael Huberman analysis model. The data validity techniques used include (1) extension of participation; (2) perseverance/grace; and (3) triangulation. Testing research data through validity testing uses product-moment correlation, while reliability testing uses the Alpha-Cronbach method. Data collection techniques used are questionnaires, participant observation, in-depth interviews, documentation, and triangulation. The results of this study show that to improve the quality of service it needs to consider five dimensions of Servqual such as facilities and infrastructure that support service performance, reliability, and responsiveness of officers in serving and helping users who need information quickly and accurately. Providing guarantees of ease in the service process and security guarantees in the library building. The strategy for developing the external environment is done by giving library staff the opportunity to participate in education and training on libraries, collaboration with other universities, improving the quality of IT-based services, and making libraries centers of information for users. While the development of the internal environment is done by improving the quality of library service systems, optimizing the addition of facilities and infrastructure such as update collections, and providing the appropriate formation of human resources in the library so that service can be optimized. A research novelty is an information packaging dimension that supports the improvement of online-based library service quality systems
Determinants of Inter-Governmental Cooperation in the Greater Jakarta Area, Indonesia
Authors: Irawan Dicky , Turmudzi M. Didi , Mulyana Yaya
Published: July 2022

This research was determined to analyze the collaboration concept execution between the regional governments in the Jabodetabekjur regions, Indonesia. The research consists of four variables; (1). starting conditions; (2). facilitative leadership; (3). institutional design ; and (4). collaborative process. Structural Equation Model (SEM) with SmartPLS is being used to analyze the data. The 87 respondents’ information were collected from various government institutions within the Jabodetabekjur regions. The results show that the collaboration amongst the government institutions in Jabodetabekjur regions were not effectively executed, due to the imbalance resources in the starting condition phase. It is shown that the DKI Jakarta (Special Capital Region of Jakarta) has more dominant financial resource that other regions.
Actualización en Dermatología
Authors: Mendoza Lopez María de Jesús , Rojas Crizón Alejandra Elizabeth , López García Marlon Andrés
Published: July 2022

La presente actualización en dermatología ofrece una lectura de indispensable valor para los médicos dermatólogos que se encuentran realizando su práctica laboral además de los médicos generales que se encuentran interesados en realizar una especialidad. Se ofrecen tres capítulos que pueden ser revisados de manera individual sin perder la estructura de la obra.
Perspectivas da pesquisa (auto)biográfica para a educação musical: um exercício metanarrativo
Authors: Almeida Jéssica de
Published: July 2022

Este artigo ensaístico tem o objetivo de refletir sobre perspectivas da pesquisa (auto)biográfica atravessadas por estudos por mim realizados nos últimos anos, através de um exercício metanarrativo. Nesse esforço, realizo contrapontos entre produções (auto)biográficas, seus fundamentos teórico-metodológicos e recentes ponderações epistemológicas possibilitadas pelo Xxxxx. Especificamente, percorro alguns fundamentos da pesquisa-formação e da biografia educativa, bem como, percepções advindas de pesquisas realizadas com essas bases e de leituras recentes que, além de atualizarem a compreensão sobre formação e pesquisa científica, fundamentam um campo epistemológico potente para pensarmos, também, sentidos para a própria música. Com isso, almejo avançar na compreensão sobre o lugar da música na pesquisa (auto)biográfica e as potências desta última para pensarmos a formação humana com e através da música.
Aspectos históricos da pesquisa em Educação Musical no Brasil pela perspectiva das trajetórias de vida-científica dos pesquisadores PQ
Authors: Oliveira Raimundo Vagner Leite de , Abreu Delmary Vasconcelos de
Published: July 2022

O presente artigo tem por objetivo refletir sobre aspectos históricos da pesquisa em Educação Musical, a partir das trajetórias de vida-científica de pesquisadores com Bolsa de Produtividade em Pesquisa/PQ do CNPq dessa área no Brasil. Como metodologia utilizou-se: a pesquisa de natureza qualitativa; a pesquisa documental; o Currículo Lattes como fonte para coleta de dados; o Biograma como referencial teórico-metodológico; e para os resultados, o paradigma interpretativo da hermenêutica para se chegar à valoração. Os resultados revelam que os caminhos trilhados por esses seis pesquisadores, apesar de singulares, convergem para um mesmo fim: consolidação e avanços constantes da Educação Musical. Isso pode ser confirmado ao observar os vários acontecimentos em suas trajetórias as quais constituem uma representação de área. São ainda formativas para outros que desejam trilhar o caminho da pesquisa e docência. Conclui-se que ao considerar a unicidade na pluralidade, é possível compreender essa área pelas trajetórias de vida-científica de pesquisadores. Dar visibilidade às trajetórias de vida-científica da área é mostrar, por esse olhar, uma das várias histórias da Educação Musical no Brasil.
Biografia e Educação Musical: um estudo sobre as rotas formativas de quatro religiosos brasileiros
Authors: Souza Jusamara Vieira , Lorenzetti Michelle Arype Girardi
Published: July 2022

O presente artigo analisa as rotas formativas musicais de quatro religiosos e suas atuações como formadores no âmbito da Igreja Católica no Brasil, na perspectiva da sociologia da educação musical. Os relatos foram colhidos por meio de entrevistas, seguindo um roteiro semiestruturado de questões que contemplaram diferentes aspectos e temas relacionados à formação e atuação com música no ambiente religioso. O referencial teórico adotado apoiou-se no conceito de “rotas” formativas, de José Machado Pais (2003), como os percursos de formação dos religiosos e seus caminhos na formação de outras pessoas. A concepção teórica de biografia e o conceito de rotas formativas possibilitaram vincular práticas e experiências individuais, no nível micro, àquelas presentes no contexto da Igreja Católica, no nível macro. Compreende-se que a formação musical dos religiosos entrevistados é impregnada pelos seus percursos biográficos revelados nas escolhas pedagógico-musicais, formas de apropriação do conhecimento musical e funções educativas e socializadoras da religião no contexto da educação musical.
Professora de música e pesquisadora: reflexividade narrativa a partir dos bastidores de uma pesquisa
Authors: Natera Gislene
Published: July 2022

Este artigo apresenta minha narrativa sobre algumas experiências que vivi no processo de uma pesquisa de doutorado desenvolvida dentro um curso de Pedagogia de uma universidade pública. O objetivo do artigo é compreender como a reflexividade narrativa contribui para o processo de formação do pesquisador. Não busco desvendar a verdade sobre os fatos, mas sim apresentar a narrativa autobiográfica, segundo Passeggi (2016, 2021) como opção metodológica para resgatar algumas experiências vividas junto com as estudantes que participaram da pesquisa e os significados que dei a elas durante e depois do processo da disciplina. Metodologicamente, narro as trilhas da pesquisa, os registros do diário de campo, as transcrições dos vídeos ou o dispositivo da memória para entender os conhecimentos e saberes tecidos nessa produção. Concluo que para tornar-me pesquisadora foi preciso aprender a silenciar, sentar-me sozinha por horas e horas em busca de fundamentação, de perguntas, respostas ou ainda de originalidade.
Exploring the experience of community health workers in COVID-19 pandemic response in Indonesia: A qualitative study
Authors: Baridwan Syamikar , Permatasari Henny , Setiawan Agus
Published: July 2022

Background: Coronavirus disease 2019 (COVID-19) is a communicable disease that has infected millions of people in many countries, including Indonesia. Community health workers (CHWs) play a pivotal role in supporting primary health center programs, including in response to the COVID-19 pandemic.Methods: We conducted semi-structured in-depth interviews with thirteen CHWs in Makassar City, Indonesia. Data were analyzed using thematic analysis.Results: Six themes emerged from the interviews: (1) self-motivation; (2) valuable benefits; (3) noble duties; (4) perceived challenges; (5) meaningful support; and (6) way forward.Conclusions: Despite the challenges experienced by the participants, the involvement of CHWs is crucial to ensure that the community receives correct information and complies with health protocols to break the chain of COVID-19 transmission. The participants undertake the task with strong commitment and the feel of honor as they are trusted by the government and the community. The partnership between health service staffs and CHWs should be strengthened by providing sufficient support so COVID-19 cases in the community can be contained.

Introducción: La enfermedad por coronavirus 2019 (COVID-19) es una enfermedad transmisible que ha infectado a millones de personas en muchos países, incluida Indonesia. Los trabajadores comunitarios de la salud (CHW, por sus siglas en inglés) desempeñan un papel fundamental en el apoyo a los programas de los centros de salud primarios, incluso en respuesta a la pandemia de COVID-19.Método: Realizamos entrevistas en profundidad semiestructuradas con 13 CHW en la ciudad de Makassar, Indonesia. Los datos se analizaron mediante análisis temático.Resultados: De las entrevistas surgieron seis temas: (1) automotivación; (2) beneficios valiosos; (3) deberes nobles; (4) desafíos percibidos; (5) apoyo significativo; y (6) camino a seguir.Conclusión: A pesar de los desafíos experimentados por los participantes, la participación de los CHW es crucial para garantizar que la comunidad reciba información correcta y cumpla con los protocolos de salud para romper la cadena de transmisión de COVID-19. Los participantes emprenden la tarea con un fuerte compromiso y el sentimiento de honor ya que cuentan con la confianza del gobierno y la comunidad. La asociación entre el personal de los servicios de salud y los CHW debe fortalecerse brindando suficiente apoyo para que se puedan contener los casos de COVID-19 en la comunidad.
Treating urinary tract infections in public sector primary healthcare facilities in Cape Town, South Africa: A pharmaceutical perspective
Authors: Keuler N , Johnson Y , Coetzee R
Published: July 2022

Background. Antibiotic resistance is a global healthcare burden complicating the management of infections. Urinary tract infections (UTIs) are commonly treated in primary care. Managing UTIs appropriately in primary care can combat antibiotic resistance. The treatment practices for UTIs in primary care in Western Cape Province, South Africa, are not well described.Objectives. To describe treatment of UTIs in adults in primary care in the Cape Town metropole public sector of the Western Cape. Method. A retrospective multicentre medical records review of patients diagnosed with UTIs was conducted during 1 October 2020 - 28 February 2021. Six public sector primary healthcare facilities were included in the study through random selection from three of the four substructures in the Cape Town metropole. Medical records of adult patients diagnosed with UTIs, through clinical diagnosis or microbiological testing, were identified via a selective sampling process. Data were collected from medical records using a standardised data collection tool.

Results. A total of 401 UTI episodes occurred in 383 patients during the study period. The majority of UTI episodes (84.3%) occurred in females, complicated UTIs (74.1%) were more common than uncomplicated UTIs, and nitrofurantoin (57.1%) was frequently prescribed, followed by ciprofloxacin (39.7%). Compliance with urine microscopy recommendations was low (6.7%), and antibiotics were appropriately selected in 75.0% of uncomplicated and 70.0% of complicated UTI episodes.

Conclusion. Interventions are required to improve compliance with treatment recommendations as per the standard treatment guidelines, especially when selecting the appropriate antibiotic, duration of therapy and urine microscopy.
MECHANOCHEMICALLY INDUCED SYNTHESIS OF La2O3
Authors: Zeljković Saša , Malinović Mladena , Sukur Sunčica , Gajić Dragana , Balaban Milica , Ostojić Gordana
Published: July 2022

Lanthanum(III)-oxide (La2O3) powders were synthesized by a mechanochemically induced solvent-deficient method using lanthanum nitrate, and ammonium bicarbonate as precursors. The precursor mixture was calcined for one hour at either 600 or 800 °C. This study included an investigation of the formation reactions, crystal structure, specific surface area, and optical properties of synthesized La2O3. The proposed mechanism indicates a complex synthesis with several reactions, some of which are mechanochemically induced. The size of the La2O3 nanocrystallites, as determined by XRD, is 22.15±3.9 nm (by using the Williamson-Hall plot, it is determined that the strain is 1.4×10−3 and crystallite size 30.81 nm) while the specific surface is set to 7.04 m2g-1. The direct bandgap value obtained from reflectance measurement is determined to be 5.37 eV.
EVALUATION OF ENAMEL SURFACE USING EDI AND ESRS INDEX AFTER REMOVAL OF THE FIXED ORTHODONTIC APPLIANCE
Authors: Arbutina Adriana , Arapović-Savić Marijana , Umićević-Davidović Mirjana , Mirjanić Vladan , Kuzmanović Radman Irena , Mirjanić Đorđe
Published: July 2022

Introduction: After completion of therapy with fixed orthodontic appliances, it is necessary to remove the brackets and the remaining adhesive. The process of removing the brackets and adhesive can lead to iatrogenic damage to the enamel surface. The aim of this research was to make a visual assessment of the enamel surface after application of the Enamel Surface Rating System and Enamel Damage Index after application of the green stone for the removal of the adhesive remnants, after completion of therapy with fixed orthodontic appliances. Material and Method: Within the research, 40 human premolars, extracted for orthodontic purposes, were collected. The back surface of all premolars was treated with 37% orthophosphoric acid and then the metal brackets were placed on 20 premolars. After the removal of the brackets, the residual adhesive was removed with a green stone. A visual assessment of the damage to the enamel was performed by the application of the Enamel Surface Rating System and the Enamel Damage Index. Results: The most commonly estimated score with EDI at the overall level was 1 (52.5%), while the most commonly estimated score by ESRS was 4 (35%). A statistically significant difference was observed between the examined groups. Conclusion: Using the ESRS index and the EDI index, significant enamel damage was determined after the application of green pebbles to remove the remaining adhesive after the completion of therapy with fixed orthodontic appliances. As part of the protocol for removing the rest of the adhesive after removing the fixed orthodontic appliance, it is recommended that the green stone be used at the very beginning of the removal of the adhesive, while the instrument is away from the viewing surface.
ROUGH PREDICTION OF OSCILLATIONS BY COMPUTING MAXIMAL INSTABILITY
Authors: Rajilić Zoran , Malivuk Gak Dragana
Published: July 2022

We use Newton’s second law of motion assuming combination chaos with stochasticity. For a measured time series, one can compute appropriate force and then better understand and roughly predict the behavior of the observed complex system. The force parameter describing instability is of the highest importance. We consider some mechanical experiments and the average global temperature.
El retorno en Colombia desde sus dimensiones de análisis. Una revisión sistemática de la literatura
Authors: Egea Jiménez Carmen , Díez Jiménez Adriano , Márquez Guerra José Francisco
Published: July 2022

This article examines the scientific literature on return migration in Colombia that assesses and sheds light on the importance of this phenomenon in terms of its empirical contributions, in the first two decades of the present century. To do so, four dimensions of analysis are used as a starting point: what and who are the returnees; where they return to —the territorial scale of return from an ontological viewpoint, specifically, the territorial dimension—; the reasons for return; and the public policies of return. The bibliographic review and selection strategy was undertaken in four phases: 1) bibliographic search in indexing and abstracting systems (SIR): ALAP-OIM, DOAJ, Redalyc, Scopus, Seriunam, Clacso, Clase, LatAM-Estudios, Redib, SciELO Colombia, Biblat, Publindex-Colombia, Cepal and Web of Science; 2) purging of duplicate studies; 3) eligibility; and 4) specific selection. The total number of papers located was 424. Of these, a total of 127 were selected according to the inclusion-exclusion and methodological evaluation criteria. Despite the progress made, we concluded that there is a lack of studies on return migration in areas such as family, multidimensional reintegration, transnationalism, and political participation. There are also few studies that address current return from Venezuela, making it difficult to design policies to manage the phenomenon. Finally, the originality of this article consists in the dimensions of the analysis of return and its temporality. This in the light of a methodological procedure using grounded theory, in which each scientific text constituted a heuristic and hermeneutic unit of comparative analysis.
β2-subunit alternative splicing stabilizes Cav2.3 Ca2+ channel activity during continuous midbrain dopamine neuron-like activity
Authors: Siller Anita , Hofer Nadja T , Tomagra Giulia , Burkert Nicole , Hess Simon , Benkert Julia , Gaifullina Aisylu , Spaich Desiree , Duda Johanna , Poetschke Christina , Vilusic Kristina , Fritz Eva Maria , Schneider Toni , Kloppenburg Peter , Liss Birgit , Carabelli Valentina , Carbone Emilio , Ortner Nadine Jasmin , Striessnig Jörg
Published: July 2022

In dopaminergic (DA) Substantia nigra (SN) neurons Cav2.3 R-type Ca2+-currents contribute to somatodendritic Ca2+-oscillations. This activity may contribute to the selective degeneration of these neurons in Parkinson’s disease (PD) since Cav2.3-knockout is neuroprotective in a PD mouse model. Here, we show that in tsA-201-cells the membrane-anchored β2-splice variants β2a and β2e are required to stabilize Cav2.3 gating properties allowing sustained Cav2.3 availability during simulated pacemaking and enhanced Ca2+-currents during bursts. We confirmed the expression of β2a- and β2e-subunit transcripts in the mouse SN and in identified SN DA neurons. Patch-clamp recordings of mouse DA midbrain neurons in culture and SN DA neurons in brain slices revealed SNX-482-sensitive R-type Ca2+-currents with voltage-dependent gating properties that suggest modulation by β2a- and/or β2e-subunits. Thus, β-subunit alternative splicing may prevent a fraction of Cav2.3 channels from inactivation in continuously active, highly vulnerable SN DA neurons, thereby also supporting Ca2+ signals contributing to the (patho)physiological role of Cav2.3 channels in PD.
Combining hypothesis- and data-driven neuroscience modeling in FAIR workflows
Authors: Eriksson Olivia , Bhalla Upinder Singh , Blackwell Kim T , Crook Sharon M , Keller Daniel , Kramer Andrei , Linne Marja-Leena , Saudargienė Ausra , Wade Rebecca C , Hellgren Kotaleski Jeanette
Published: July 2022

Modeling in neuroscience occurs at the intersection of different points of view and approaches. Typically, hypothesis-driven modeling brings a question into focus so that a model is constructed to investigate a specific hypothesis about how the system works or why certain phenomena are observed. Data-driven modeling, on the other hand, follows a more unbiased approach, with model construction informed by the computationally intensive use of data. At the same time, researchers employ models at different biological scales and at different levels of abstraction. Combining these models while validating them against experimental data increases understanding of the multiscale brain. However, a lack of interoperability, transparency, and reusability of both models and the workflows used to construct them creates barriers for the integration of models representing different biological scales and built using different modeling philosophies. We argue that the same imperatives that drive resources and policy for data – such as the FAIR (Findable, Accessible, Interoperable, Reusable) principles – also support the integration of different modeling approaches. The FAIR principles require that data be shared in formats that are Findable, Accessible, Interoperable, and Reusable. Applying these principles to models and modeling workflows, as well as the data used to constrain and validate them, would allow researchers to find, reuse, question, validate, and extend published models, regardless of whether they are implemented phenomenologically or mechanistically, as a few equations or as a multiscale, hierarchical system. To illustrate these ideas, we use a classical synaptic plasticity model, the Bienenstock–Cooper–Munro rule, as an example due to its long history, different levels of abstraction, and implementation at many scales.
Neural mechanisms underlying the temporal organization of naturalistic animal behavior
Authors: Mazzucato Luca
Published: July 2022

Naturalistic animal behavior exhibits a strikingly complex organization in the temporal domain, with variability arising from at least three sources: hierarchical, contextual, and stochastic. What neural mechanisms and computational principles underlie such intricate temporal features? In this review, we provide a critical assessment of the existing behavioral and neurophysiological evidence for these sources of temporal variability in naturalistic behavior. Recent research converges on an emergent mechanistic theory of temporal variability based on attractor neural networks and metastable dynamics, arising via coordinated interactions between mesoscopic neural circuits. We highlight the crucial role played by structural heterogeneities as well as noise from mesoscopic feedback loops in regulating flexible behavior. We assess the shortcomings and missing links in the current theoretical and experimental literature and propose new directions of investigation to fill these gaps.
Taste quality and hunger interactions in a feeding sensorimotor circuit
Authors: Shiu Philip K , Sterne Gabriella R , Engert Stefanie , Dickson Barry J , Scott Kristin
Published: July 2022

Taste detection and hunger state dynamically regulate the decision to initiate feeding. To study how context-appropriate feeding decisions are generated, we combined synaptic resolution circuit reconstruction with targeted genetic access to specific neurons to elucidate a gustatory sensorimotor circuit for feeding initiation in adult Drosophila melanogaster. This circuit connects gustatory sensory neurons to proboscis motor neurons through three intermediate layers. Most neurons in this pathway are necessary and sufficient for proboscis extension, a feeding initiation behavior, and respond selectively to sugar taste detection. Pathway activity is amplified by hunger signals that act at select second-order neurons to promote feeding initiation in food-deprived animals. In contrast, the feeding initiation circuit is inhibited by a bitter taste pathway that impinges on premotor neurons, illuminating a local motif that weighs sugar and bitter taste detection to adjust the behavioral outcomes. Together, these studies reveal central mechanisms for the integration of external taste detection and internal nutritive state to flexibly execute a critical feeding decision.
Characterization of phenylalanine ammonia-lyase genes facilitating flavonoid biosynthesis from two species of medicinal plant Anoectochilus
Authors: Yang Lin , Li Wan-Chen , Fu Feng-ling , Qu Jingtao , Sun Fuai , Yu Haoqiang , Zhang Juncheng
Published: July 2022

Background

Anoectochilus roxburghii and Anoectochilus formosanus, belong to the Anoectochilus genus, have been used for Chinese herbal drugs as well as health food. Phenylalanine ammonia-lyase (PAL), the key enzyme in primary metabolism and phenylpropanoid metabolism, produces secondary metabolites (flavonoids) in plants, which are beneficial for the biosynthesis of phenylpropanoid metabolites.

Methods

The PAL genes were cloned from A. formosanus and A. roxburghii according to our previous transcriptomic analysis. The PALs were introduced into pCAMBIA2300-35S-PAL-eGFP to generate 35S-PAL-eGFP. The constructs were further used for subcellular localization and transgenic Arabidopsis. The expression of AfPAL and ArPAL under precursor substance (L-Phe), NaCl, UV, and red-light were analyzed by real-time quantitative PCR (RT-qPCR).

Results

AfPAL and ArPAL , encoding 2,148 base pairs, were cloned from A. formosanus and A. roxburghii. The subcellular localization showed that the ArPAL and AfPAL were both localized in the nucleus with GPF. Quantitative RT-PCR analysis indicated that the ArPAL and AfPAL genes function in the phenylalanine pathway as well as response to induced conditions. Overexpression of the AfPAL and ArPAL could increase flavonoids and anthocyanin content in the transgenic Arabidopsis.

Discussion

The results suggest that AfPAL and ArPAL play a crucial role in the flavonoid biosynthesis in Anoectochilus. Also, our study provides new insights into the enrichment of secondary metabolites of traditional Chinese medicines A. formosanus and A. roxburghii, which can improve their medicinal active ingredients and be used for drug discovery in plants.
Evaluation of potential anti-metastatic and antioxidative abilities of natural peptides derived from Tecoma stans (L.) Juss. ex Kunth in A549 cells
Authors: Krobthong Sucheewin , Yingchutrakul Yodying , Sittisaree Wattanapong , Tulyananda Tatpong , Samutrtai Pawitrabhorn , Choowongkomon Kiattawee , Lao-On Udom
Published: July 2022

Background

Tecoma stans (L.) Juss. ex Kunth is a well-known medicinal plant found in tropical and subtropical regions. It contains a broad range of bioactive compounds that exhibit many biological effects, including antidiabetic, antibacterial, and antioxidative activities. However, the effect of natural peptides from T. stans against cancer progression and free radical production is unknown. This study aims to evaluate the cytotoxic, anti-metastatic, and antioxidative activities of natural peptides from T. stans on A549 cells.

Methods

The natural peptides were extracted from the flower of T. stans using the pressurized hot water extraction (PHWE) method, followed by size exclusion chromatography and solid-phase extraction-C18. The cytotoxic and anti-metastatic effects of natural peptides were evaluated using MTT and transwell chamber assays, respectively. The free radical scavenging activity of natural peptides was determined using ABTS, DPPH, and FRAP assays. The cells were pretreated with the IC50 dosage of natural peptides and stimulated with LPS before analyzing intracellular reactive oxygen species (ROS) and proteomics.

Results

Natural peptides induced cell toxicity at a concentration of less than 1 ng/ml and markedly reduced cell motility of A549 cells. The cells had a migration rate of less than 10% and lost their invasion ability in the treatment condition. In addition, natural peptides showed free radical scavenging activity similar to standard antioxidants and significantly decreased intracellular ROS in the LPS-induced cells. Proteomic analysis revealed 1,604 differentially expressed proteins. The self-organizing tree algorithm (SOTA) clustered the protein abundances into eleven groups. The volcano plot revealed that the cancer-promoting proteins (NCBP2, AMD, MER34, ENC1, and COA4) were down-regulated, while the secretory glycoprotein (A1BG) and ROS-reducing protein (ASB6) were up-regulated in the treatment group.

Conclusion

The anti-proliferative and anti-metastatic activities of natural peptides may be attributed to the suppression of several cancer-promoting proteins. In contrast, their antioxidative activity may result from the up-regulation of ROS-reducing protein. This finding suggests that natural peptides from T. stans are viable for being the new potential anti-cancer and antioxidative agents.
Ferroptotic cardiomyocyte-derived exosomes promote cardiac macrophage M1 polarization during myocardial infarction
Authors: Sun Shengjia , Wu Yurong , Maimaitijiang Alimujiang , Huang Qingyu , Chen Qiying
Published: July 2022

Ferroptosis is a mode of cell death that occurs in myocardial infarction (MI). Signals emanating from apoptotic cells are able to induce macrophage polarization through exosome-loading cargos, which plays a vital role in the process of disease. However, whether ferroptotic cardiomyocytes derived exosome (MI-Exo) during MI act on macrophage polarization and its mechanism remain unclear. In this study, a MI mouse model was established, and cardiac function evaluation and pathological staining were performed. The effect of MI-Exo on polarization of RAW264.7 cells was assessed by the expression of IL-10 and NOS2. Ferroptosis inhibitor of ferrostatin-1 was used to verify whether MI-Exo function was dependents on ferroptosis. Cardiac function and myocardial histomorphology were markedly impaired and massive immune cell infiltration in MI mice, compared with the sham group. The significantly increased MDA content and Fe2+ accumulation in the heart tissue of MI mice suggested cardiomyocyte ferroptosis. Compared with the sham group, the expression of M1 marker NOS2 was significantly up-regulated and M2 marker IL-10 was significantly down-regulated in the heart tissue of MI mice. Exosome-derived from MI HL-1 cell-treated with ferrostatin-1 (Fer-1-Exo) and MI-Exo were internalized by RAW 264.7 cells. Compared with culture alone, co-cultured with MI-Exo significantly promoted NOS2 expression and suppressed IL-10 expression, and decreased proportion of Arginase-1-labeled M2 macrophages, also inhibited phagocytosis of RAW 264.7 cells. Wnt1 and β-cantenin expression also elevated after treated with MI-Exo. However, co-cultured with Fer-1-Exo significantly reversed the above changes on RAW 264.7 cells induced by MI-Exo. In conclusion, ferroptotic cardiomyocytes-derived exosome crosstalk macrophage to induce M1 polarization via Wnt/β-cantenin pathway, resulting in pathological progress in MI. This understanding provides novel therapeutic target for MI.
Investigation on Effects of Water Mist Characteristics According to Axial Position on Thermal Radiation Attenuation Performance
Authors: Kang Jun Seok , Lee Chi Young
Published: July 2022

In this study, the effects of water mist characteristics according to the axial position on thermal radiation attenuation performance were experimentally investigated using the single-fluid nozzle. Under the water flow rate conditions of 200∼350 g/min, the thermal radiation attenuation performance was measured at the axial position (i.e., downstream direction of water mist from nozzle exit) of 200∼1000 mm. In addition, during the discharge of water mist, the water supply pressure and droplet size of water mist were measured and the water mist was visualized. As a result, with an increase in the water flow rate, the thermal radiation attenuation performance was improved. Overall, the attenuation rate was measured to be 12.4∼30.1%. In the axial position of 200∼400 mm, with an increase in the axial distance from the nozzle exit, the thermal radiation attenuation performance was improved. This may be because the effect of improvement of the thermal radiation attenuation performance by an increase in the spray width is predominant over the effect of reduction in it by an increase in the droplet size. In addition, in the axial position of 400∼1000 mm, with an increase in the axial distance from the nozzle exit, the thermal radiation attenuation performance was reduced. This is because the droplet size of water mist increases and spray width is narrowed. Based on this study, it was confirmed that the water mist characteristics according to the axial position and thermal radiation attenuation performance are closely correlated.
Development of the Educational Program for Fire Officials’ Peer Psychological Support
Authors: Hur Mira , Jung In Chul
Published: July 2022

This study aims to develop an educational program for peer psychological support in the fire officials’ organization. The program was developed through goal setting, component derivation, pilot study, model design, and effectiveness verification. Forty fire officials were divided into an experimental group and a control group of twenty people each. The program was conducted with a total of twelve sessions. Empathy, self-reflection, and interpersonal skill levels were examined before and after the program. The results indicated that the levels of empathy and self-reflection of the experimental group were meaningfully improved (F = 5.96, 5.45, p < .05) compared to the control group, but interpersonal relationship was not (F = 2.88, p < .05). This study makes a meaningful contribution through developing of program using a scientific and systematic method in the field of psychological education and counseling.