bluuebunny/crossref_metadata_2025 huggingface dataset:
Trust and privacy in the context of user-generated health data
Authors: Ostherr Kirsten , Borodina Svetlana , Bracken Rachel Conrad , Lotterman Charles , Storer Eliot , Williams Brandon
Published: April 2017

This study identifies and explores evolving concepts of trust and privacy in the context of user-generated health data. We define “user-generated health data” as data captured through devices or software (whether purpose built or commercially available) and used outside of traditional clinical settings for tracking personal health data. The investigators conducted qualitative research through semistructured interviews (n = 32) with researchers, health technology start-up companies, and members of the general public to inquire why and how they interact with and understand the value of user-generated health data. We found significant results concerning new attitudes toward trust, privacy, and sharing of health data outside of clinical settings that conflict with regulations governing health data within clinical settings. Members of the general public expressed little concern about sharing health data with the companies that sold the devices or apps they used, and indicated that they rarely read the “terms and conditions” detailing how their data may be exploited by the company or third-party affiliates before consenting to them. In contrast, interviews with researchers revealed significant resistance among potential research participants to sharing their user-generated health data for purposes of scientific study. The widespread rhetoric of personalization and social sharing in “user-generated culture” appears to facilitate an understanding of user-generated health data that deemphasizes the risk of exploitation in favor of loosely defined benefits to individual and social well-being. We recommend clarification and greater transparency of regulations governing data sharing related to health.
Genome sequencing and analysis of Ralstonia solanacearum phylotype I strains FJAT-91, FJAT-452 and FJAT-462 isolated from tomato, eggplant, and chili pepper in China
Authors: Sun Yidan , Wang Keke , Caceres-Moreno Carlos , Jia Wei , Chen Aojun , Zhang Heng , Liu Renyi , Macho Alberto P.
Published: April 2017

Ralstonia solanacearum is an extremely destructive pathogen able to cause disease in a wide range of host plants. Here we report the draft genome sequences of the strains FJAT-91, FJAT-452 and FJAT-462, isolated from tomato, eggplant, and chili pepper, respectively, in China. In addition to the genome annotation, we performed a search for type-III secreted effectors in these strains, providing a detailed annotation of their presence and distinctive features compared to the effector repertoire of the reference phylotype I strain (GMI1000). In this analysis, we found that each strain has a unique effector repertoire, encoding both strain-specific effector variants and variations shared among all three strains. Our study, based on strains isolated from different hosts within the same geographical location, provides insight into effector repertoires sufficient to cause disease in different hosts, and may contribute to the identification of host specificity determinants for R. solanacearum.
Passive seismic monitoring with nonstationary noise sources
Authors: Delaney Evan , Ermert Laura , Sager Korbinian , Kritski Alexander , Bussat Sascha , Fichtner Andreas
Published: April 2017

Heterogeneous, nonstationary noise sources can cause traveltime errors in noise-based seismic monitoring. The effect worsens with increasing temporal resolution. This may lead to costly false alarms in response to safety concerns and limit our confidence in the results when these systems are used for quasi-real-time monitoring of subsurface changes. Therefore, we have developed a new method to quantify and correct these traveltime errors to more accurately monitor subsurface conditions at daily or even hourly timescales. It is based on the inversion of noise correlation asymmetries for the time-dependent distribution of noise sources. The source model is then used to simulate time-dependent ambient noise correlations. The comparison with correlations computed for homogeneous noise sources yields traveltime errors that translate into spurious changes of the subsurface. The application of our method to data acquired at Statoil’s SWIM array, a permanent seismic installation at the Oseberg field, demonstrates that fluctuations in the noise source distribution may induce apparent velocity changes of 0.25% within one day. Such biases thereby likely mask realistic subsurface variations expected on these timescales. These errors are systematic, being primarily dependent on the noise source location and strength, and not on the interstation distance. Our method can then be used to correct for source-induced traveltime errors by subtracting these quantified biases in either the data or model space. It can furthermore establish a minimum threshold for which we may reliably attribute traveltime changes to actual subsurface changes, should we not correct for these errors. In addition to the aforementioned real data scenario, we apply our method to a synthetic case for a daily passive monitoring overburden feasibility study. Synthetics and field experiments validated the method’s theory and application.
Central Hypothyroidism Due to a TRHR Mutation Causing Impaired Ligand Affinity and Transactivation of Gq
Authors: García Marta , González de Buitrago Jesús , Jiménez-Rosés Mireia , Pardo Leonardo , Hinkle Patricia M. , Moreno José C.
Published: April 2017

Context:

Central congenital hypothyroidism (CCH) is an underdiagnosed disorder characterized by deficient production and bioactivity of thyroid-stimulating hormone (TSH) leading to low thyroid hormone synthesis. Thyrotropin-releasing hormone (TRH) receptor (TRHR) defects are rare recessive disorders usually associated with incidentally identified CCH and short stature in childhood.

Objectives:

Clinical and genetic characterization of a consanguineous family of Roma origin with central hypothyroidism and identification of underlying molecular mechanisms.

Design:

All family members were phenotyped with thyroid hormone profiles, pituitary magnetic resonance imaging, TRH tests, and dynamic tests for other pituitary hormones. Candidate TRH, TRHR, TSHB, and IGSF1 genes were screened for mutations. A mutant TRHR was characterized in vitro and by molecular modeling.

Results:

A homozygous missense mutation in TRHR (c.392T > C; p.I131T) was identified in an 8-year-old boy with moderate hypothyroidism (TSH: 2.61 mIU/L, Normal: 0.27 to 4.2; free thyroxine: 9.52 pmol/L, Normal: 10.9 to 25.7) who was overweight (body mass index: 20.4 kg/m2, p91) but had normal stature (122 cm; –0.58 standard deviation). His mother, two brothers, and grandmother were heterozygous for the mutation with isolated hyperthyrotropinemia (TSH: 4.3 to 8 mIU/L). The I131T mutation, in TRHR intracellular loop 2, decreases TRH affinity and increases the half-maximal effective concentration for signaling. Modeling of TRHR-Gq complexes predicts that the mutation disrupts the interaction between receptor and a hydrophobic pocket formed by Gq.

Conclusions:

A unique missense TRHR defect identified in a consanguineous family is associated with central hypothyroidism in homozygotes and hyperthyrotropinemia in heterozygotes, suggesting compensatory elevation of TSH with reduced biopotency. The I131T mutation decreases TRH binding and TRHR-Gq coupling and signaling.
Figures of the Visual: Japanese Modernism, Technology, Vitalism
Authors: Hayter Irena
Published: April 2017

This essay focuses on perception and the senses in Japanese literary modernism and specifically in the so-called new sensationist (shinkankakuha) writers, especially Kawabata Yasunari (1899–1972) and Yokomitsu Riichi (1898–1947). The critical debates that unfolded around their modernist journal Bungei jidai (Literary Age) (1924–27) were preoccupied with attempts to explain and philosophically anchor notions of sensation and perception. This did not mean an overwhelming concern with the body and its senses: on the contrary, in these texts perception emerges as somehow purified and reduced to vision, that most decorporealized of the senses. Indeed, the fragmented style, the distorted temporalities, and the deinteriorized subjects of Kawabata and Yokomitsu owe a lot to the visual, and especially to the technologized visuality of film. Paradoxically, however, this fractured cinematic aesthetics was grafted onto older visions of wholeness, some of them ideologically vulnerable. Cutting through literary studies, film, and intellectual history, domains normally kept apart, the essay traces those motifs in Kawabata and Yokomitsu that speak not of modernist rupture but of a deeper continuity with the powerful vitalist ideas from the turn of the century, such as the urge to dissolve subject-object dichotomies into an “Eastern monism” or a mystical life force and the animistic figuring of technology. New sensationism emerges as a discursive field shot through with tensions and nonsynchronicities. A supposedly avant-garde engagement with urban exteriority from its very beginnings contained an ambiguous spiritualism.
In the Beginning: Blackness and the 1960s Creative Nonfiction of Ōe Kenzaburō
Authors: Bridges William H.
Published: April 2017

This essay considers the nonfictional writings on black literature and American race relations penned by Nobel laureate Ōe Kenzaburō between the years 1961 (when Ōe attended the Asian-African Writers Conference held in Tokyo) and 1968 (when he delivered a series of speeches in Kinokuniya Hall on American race relations). In these nonfictional musings, Ōe posits an analogous existential dilemma shared by the postwar Japanese and post–civil rights era African-Americans. Ōe's proposed solution to this dilemma—a kind of existential freedom rooted in celebrating ethnic and racial diversity—is highly informed by his reading of Richard Wright, Ralph Ellison, and James Baldwin.
Tanigawa Gan and the Poetics of the Origin
Authors: Walker Gavin
Published: April 2017

The work of the poet, theoretician, and political activist Tanigawa Gan (1923–95) played a decisive role in postwar Japanese intellectual and political life, deeply influencing the politics of 1968. By interrupting the prevailing discourses of democratic reconstruction, high economic growth, and the cosmopolitan Marxism of urban life, Tanigawa (along with the other members of his commune) practiced a rigorous subtraction from the dominant ideological and aesthetic discourse of postwar Japanese life. Remapping their politics by imbricating activism with the line of writing, they moved into the rural and isolated villages, the utterly deprived and depleted “forgotten” zones of the countryside, where Tanigawa attempted to discover an “origin,” not in a romantic or nativist sense, but as a source of human energy for the active aesthetic creation of life practices in exodus from the dominant society. Tanigawa's writing itself—his practice of holding politics and poetics immanent to the flux or energy of the historicity of the struggle of the oppressed, to the historical event of their dispossession—remains a powerful site of analysis for us today. In contemporary Japan, where the struggles of the unemployed, the homeless, and the semipermanently underemployed have emerged sharply against the backdrop of the turn to neoliberalism and dismantling of social policy, Tanigawa's work has seen a sudden resurgence. But his work is frequently being presented as “utopian,” “nativist,” “romantic,” and so forth. In examining a sequence of Tanigawa's poetry and certain of his critical essays, the author tries to disrupt such readings by arguing that his attempt to conceive of a practice of writing, not focused on the substantialization of “the people” or the region but, rather, on the dynamic flux of energy produced by this tension of exile itself, inaugurates a series of potential moments through which the possibilities of resistant mappings can be understood. For Tanigawa, the political potential of this mapping rather lies in mobilizing the histories of the affective memory of struggle that are sedimented in the communities of the dispossessed, what he called “operating” (kosaku) on the situation.
Photography and Invisibility: Indexicality and Performativity Asia-Pacific War Memory in Tsukada Mamoru'sIdentical TwinsSeries (2003) and Suzuki Norio's Photos of Onoda Hiroo (1974)
Authors: Zohar Ayelet
Published: April 2017

This manuscript engages with two central ideas: the first concerns issues related to the problem of truth value in photography, vis-à-vis issues of fictionality and the powers of the false. This discussion is set forth through the analysis of two photographic series—Tsukada Mamoru's Identical Twins (2003), a series of staged photographs of two young men dressed alternately in Japan's imperial military uniform and modern casual dress, posing in a jungle setting, coinciding with the public debates on Japan's possible involvement in the Iraq war in 2003. The author utilizes the ideas and questions raised through this series to understand the powers and position of the second series, Suzuki Norio's 1974 photographs of Onoda Hiroo, the Japanese straggler who stayed for three decades in the jungles of Lubang Island—photographs that were directly responsible for Onoda's repatriation to Japan. The discussion in the text also illuminates a second idea—how complex is Japan's position toward the trauma of defeat and the specters of war, reflecting the complicated emotions experienced toward war memory and its representation in photographs, films, art, and other visual forms. The analysis of the two series delves into issues of photography theory, on questions of indexicality and performativity, and the relations between photographic images in specific cultural climate. The author analyzes how these images may indicate the political fluctuations between the Asia-Pacific War, the 1970s, and 2003, looking at such issues as indexicality and performativity, ghostly presences and staged scenes, truth and fiction, using photography as an immediate visual reference. Through the text, political, social, and cultural questions concerning wartime Japan are intertwined with an analysis of the visual methods, creating a complex view of visual representation and its means and methods.
ENDOMETRIOSIS OF POSTOPERATIVE CICATRIX
Authors: ���������  , Florensov Vladimir , �������  , Petukhov Andrey , �����  , Rudaya Viktoriya
Published: April 2017

Rare occurrence, absence of specific clinical manifestations, difficult diagnostics on early stages, and insufficient experience in treatment of endometriosis genitalis externa deserve special attention. The study summarizes the results of treatment of scar endometriosis in four patients (three of reproductive and one of late reproductive age) describing clinical and diagnostic peculiarities of all cases. We revealed no specific laboratory diagnostic criteria of the pathology. The article is illustrated with photos of ultrasound findings, surgical field, a fragment of scar tissue removed during the surgery, and photomicrographies of surgical specimen histological sections. All presented pictures are described with indication of pathological process markers. We have stated the discrepancy between the sizes of endometrioid infiltrations in anterior abdominal wall revealed with echosonography and the sizes of those found during the surgery. During the surgery, we discovered that parietal peritoneum was not involved in pathological process. Basing on our observations, we have made recommendations on formation of scar endometriosis risk groups. In all four cases, surgical treatment with succeeding course of hormone therapy resulted in recovery of the patients.
ESTIMATION OF REPRODUCTIVE TOXICITY OF THE COMPLEX PLANT REMEDY �TIREOTON�
Authors: ������  , Ubeeva Iraida , ��������  , Nikolaev Sergey , ���������  , Razuvaeva Yanina , ������  , Verlan Nadezhda , �������  , Ayusheeva Viktoriya
Published: April 2017

To study the probable negative influence of the complex remedy �Tireoton� on the reproductive function of the laboratory

animals the following tests have been carried out, namely the probable occurrence of embryo-toxicity, teratogenicity,

foetotoxicity and its influence on the postnatal development of the laboratory animals progeny. The experiments have

been carried out of the white Wistar rats. It has been established that the �Tireoton� at the doses of 75 and 750 mg/ kg

has no embryotoxic, teratogenic and fetotoxical effect. The introduction of the �Tireoton� at the dose of 750 mg/kg

since 6th to 19th day of pregnancy does not influence on the postnatal development of white rats progeny.
COMPARATIVE EVALUATION OF HEART RATE VARIABILITY AFTER SEPTOPLASTY AND TONSILLECTOMY
Authors: Старцева  , Startseva Tatyana , Ильинская  , Ilinskaya Marina , Торшин  , Torshin Vladimir , Шевелев  , Shevelev Oleg , Попадюк  , Popadyuk Valentin , Демина  , Demina Elena , Кастыро  , Kastyro Igor , Ключникова  , Klyuchnikova Olga
Published: April 2017

The aim of the research was to determine the fluctuations of the autonomic nervous system based on the analysis of changes in certain heart rate variability (HRV) at patients after septoplasty and tonsillectomy. A study of Holter monitoring of 28 patients with nasal septum deviation and of 30 patients with chronic tonsillitis was conducted. Surgical procedures were performed under local infiltration anesthesia with 0,5% novocaine solution. We evaluated standard deviation of RR intervals (SDNN) – 24-hour monitoring, day and night data. After septoplasty for night and day validity period average SDNN did not differ (p < 0,05), but SDNN were significantly different (p < 0,05). Average values for the night and day were different from each other at the patients after tonsillectomy (p < 0,05). SDNN scatter was the same. SDNN in both groups did not differ per day both for average values and for variability (p < 0,05). Septoplasty provokes more pronounced stress response as compared with tonsillectomy. Local anesthesia for septoplasty and tonsillectomy causes a disturbance of adaptive reactions on the part of the autonomic nervous system. HRV analysis can be called an objective and individual method of diagnosis of acute stress conditions in the otorhinolaryngological surgery.
FEATURES OF INTENSIVE ORGAN-SPARING THERAPY FOR MASSIVE POSTPARTUM HEMORRHAGE
Authors: Сверкунова  , Sverkunova Nataliya , Ежова  , Ezhova Irina , Крамарский  , Kramarskiy Vladimir , Дудакова  , Dudakova Viktoriya
Published: April 2017

Bleeding in pregnancy, childbirth and the postpartum period is one of the leading causes of maternal morbidity and mortality worldwide. There is no doubt that obstetric bleeding demands the development of improved methods for its diagnostics and treatment. We assessed the effectiveness of the treatment strategy for massive postpartum hemorrhage (PPH) with preservation of reproductive function, applied in the Perinatal Center of Irkutsk. We performed a retrospective analysis of 24 delivery cases, complicated by massive bleeding and successfully treated with preservation of reproductive organs. The massive nature of bleeding (≥ 30 % of blood volume) was registered in 15 (62.5 %) cases, mild (≥ 20 % but ≤ 30 % of blood volume) – in 9 (37.5 %). Emergency operative delivery (cesarean section) was performed in 40 % of massive PPH cases and in 66.7 % of mild PPH cases. Uterotonic drugs were used in all PPH cases. When analyzing complex mechanical methods of PPH arrest, we noticed that in 20 % of cases balloon tamponade was used. In 33.3 % of mild PPH cases, balloon tamponade with great vessels ligation was performed. Thus, the most effective method was balloon tamponade coupled with Baksheev forceps and early surgical hemostasis. All in all, prevention of hemorrhage in risk groups and early fluid and surgical therapy, and also administration of uterotonic drugs in PPH treatment not only provide a hemostatic effect but also preserve reproductive function.
ANALYSIS OF THE EFFICIENCY OF IFV TECHNIQUES (ICSI, PICSI) IN THE ASSISTED REPRODUCTIVE TECHNOLOGY PROGRAMS
Authors: Одареева  , Odareeva Elena , Болдонова  , Boldonova Natalya , Мыльникова  , Mylnikova Yuliya , Маслова  , Maslova Darya , Дружинина  , Druzhinina Elena , Протопопова  , Protopopova Natalya , Мащакевич  , Mashchakevich Lyubov
Published: April 2017

The male factor is the cause of infertility in 40 % of cases. This study is dedicated to the efficiency assessment of ICSI
and PICSI techniques and the determination of indications of these methods. There are some relative indications for ICSI
in clinical practice: advanced reproductive age of patients, a small number of obtained oocytes, prolonged infertility,
repeated attempts of in vitro fertilization. However, normal sperm values are registered in 71.3 % of cases. PICSI method
is more preferable at high level of DNA fragmentation and associated changes in sperm indicators. Nevertheless, the
pregnancy rate after these methods was comparable – 19.3 % and 19.7 % respectively. The results indicate that the ICSI
and PICSI methods do not have a negative impact on the quality of the embryos and do not increase the pregnancy rate.
INFLUENCE OF SANATORIUM TREATMENT WITH THERMOTHERAPY ON THE ADAPTIVE CAPACITY OF HARD-WORKING PEOPLE
Authors: Поспелова  , Pospelova Olga , Соловьева  , Soloveva Tatyana , Федотченко  , Fedotchenko Aleksandr , Камека  , Kameka Denis
Published: April 2017

370 apparently healthy men aged 27 to 35 with considerable mental and physical burden were examined at the sanatorium.
They were divided into two groups depending on the duration of rehabilitation. Group 1 consisted of 226
men with rehabilitation period from 18 to 21 days. Group 2 comprised 144 men with rehabilitation period from 10 to
14 days. Each group was subdivided into three subgroups: A, B and C. Patients of Group 1 subgroup A visited cryochamber,
patients of Group 1 subgroup B visited infrared sauna, and patients of Group 1 subgroup C visited vibration sauna
every second day. Patients of Group 2 subgroups A, B and C had the same procedures every day. Dynamic indicators of
nonspecific adaptive response, hemodynamics, vascular adrenergic reactivity of skin, blood coagulation system, lipid
and carbohydrate metabolism of all patients were studied. The results allowed us to conclude that all therapeutic
complexes could be used in the restoration of adaptive capacity of hard-working people. Cryotherapy performed every
second day was the most effective.
HYGIENIC FEATURES OF CHEMICAL AIR POLLUTION IN ALUMINUM PRODUCTION
Authors: Меринов  , Merinov Aleksey , Лисецкая  , Lisetskaya Lyudmila , Шаяхметов  , Shayakhmetov Salim , Мещакова  , Meshchakova Nina , Калинина  , Kalinina Oksana
Published: April 2017

The article presents retrospective assessment of chemical factors in aluminum production over a long follow-up period.
The main pollutants of the workplace air are fluorine compounds, resinous substances, and disintegration aerosol. The
comparative assessment of the workplace air composition during the operation of electrolytic cells with self-baking
anodes and when using a new technology with pre-baked anodes was carried out. The results of the research were
processed using standard parametric methods of calculation of mean value and error in mean. It is shown that the
introduction of a new technology of pre-baked anodes contributes to the optimization of working environment, reduction
of harmful chemicals in the workplace air.
DETECTION OF BABESIA AND ANAPLASMATACEAE BACTERIA IN SMALL MAMMALS FROM IRKUTSK AND NOVOSIBIRSK REGIONS
Authors: Козлова  , Kozlova Irina , Лисак  , Lisak Oksana , Панов  , Panov Viktor , Тикунов  , Tikunov Artem , Сунцова  , Suntsova Olga , Федулина  , Fedulina Olga , Рар  , Rar Vera , Тикунова  , Tikunova Nina
Published: April 2017

Tissue samples from 228 small mammals captured from 2013 to 2015 in four districts of Irkutsk region and in one
district of Novosibirsk region were examined for the presence of Anaplasmataceae bacteria and Babesia parasites by
nested PCR method with subsequent sequencing of positive samples. In Ekhirit-Bulagatskiy District of Irkutsk region,
Babesia microti DNA was found in 10.9 % of small mammals, Anaplasma phagocytophilum DNA – in 7.3 %, and Ehrlichia
muris DNA – in 1.8 %. Infected mammals were not found in the other three examined districts of Irkutsk region.
In Novosibirsk region, B. microti DNA was found in 8.9 % of small rodents, A. phagocytophilum DNA – in 8.9 % of small
mammals, and Candidatus Neoehrlichia mikurensis DNA – in 8.9 % of small mammals. The identified infection agents
corresponded to microorganism species and genetic variants previously found in Ixodes persulcatus ticks but not in
ticks of other species.
OUR EXPERIENCE OF USING LASER VAPORIZATION FOR TREATMENT OF LUMBAR DISC HERNIATION
Authors: Ларионов  , Larionov Sergey , Потапов  , Potapov Vitaliy , Горбунов  , Gorbunov Anatoliy , Сороковиков  , Sorokovikov Vladimir , Кошкарёва  , Koshkareva Zinaida
Published: April 2017

The article presents the analysis of effectiveness of laser vaporization for treatment of pain syndrome caused by lumbar disc herniation. We treated 149patients (average age – 40years). 62(41.7%) patients had lumbodynia, 67(45.2%) patients had lumbar ischialgia. Nerve root disorders (radiculopathy, radiculoneuritis) are found in 20(13.1%)patients. 86(57.4%) patients had median hernias of intervertebral disc (disc bulging for 0.6±0.2cm), 36(24.3%) patients had intervertebral disc protrusion and 27(18.3 %) patients had lateral intervertebral hernia (disc bulging for 0.55±0.1 5cm). Satisfactory and good results of laser vaporization are registered in 140(94%) patients with circular protrusion and median hernias with disc bulging up to 7–8mm. Also the decrease in VAS scores of pain syndrome by 2–5points in comparison with initial condition is found. Unsatisfactory results are registered in 9(6%) patients with lateral hernias of more than 5mm and with disease duration more than 6months. Prospective (from 3 to 13months) follow-up was conducted in 49patients. 5 patients from the group with lateral hernias needed open surgeries.
PORTO-HEPATIC HEMODYNAMICS IN PATIENTS WITH DESTRUCTIVE PANCREATITIS COMBINED WITH LIVER FAILURE
Authors: Плеханов  , Plekhanov Aleksandr , Решетников  , Reshetnikov Denis
Published: April 2017

Extraorgan complications of acute destructive pancreatitis are registered in 60–95% of patients. Liver failure is regis-tered in one of the four patients with pancreatic necrosis. We analyzed the results of surgical treatment of 122patients with acute destructive pancreatitis. We studied clinical indicators of liver failure in 103(84.4%) patients with acute pancreatitis. The development of portal hypertension with decrease in hepatic volume perfusion is an important ele-ment in the pathogenesis of acute pancreatitis and associated liver function abnormalities. In patients with severe and moderate liver failure, the reduction of blood flow in portal vein was registered. It was caused by reduce of portal vein diameter and decrease in linear blood flow velocity. Destructive pancreatitis is accompanied by liver failure with hyperbilirubinemia, increased activity of cytolytic enzymes, incoordination in protein and carbohydrate metabolism as its common manifestations. An important factor in the pathogenesis of liver failure is a reduction of porto-hepatic hemodynamics and as a consequence – hepatic hypoperfusion.
ANALYSIS OF THE RESULTS OF STAGING DECOMPRESSION/STABILIZATION INTERVENTIONS IN THE TREATMENT OF PATIENTS WITH TANDEM STENOSIS OF CERVICAL AND LUMBAR SPINE
Authors: Шепелев  , Shepelev Valeriy , Сороковиков  , Sorokovikov Vladimir , Калинин  , Kalinin Andrey , Бывальцев  , Byvaltsev Vadim , Крутько  , Krutko Aleksandr
Published: April 2017

The concept of “tandem stenosis” comprises combination of degenerative stenosis in both cervical and lumbar spine. At the moment, there is no consistent approach to the surgical procedures in this group of patients, as well as the research-ers do not pay much attention in their papers to the sequence and stage-by-stage approach to the procedures and to the analysis of combined surgical procedures. The aim of the research was to analyze the results of staging decompression/stabilization interventions in the treatment of patients with tandem stenosis of cervical and lumbar spine. We performed a retrospective analysis of prospectively collected results of treatment of 46patients with combined (tandem) stenosis of cervical and lumbar spine. The patients were operated in four neurosurgical clinical units of Vladivostok, Irkutsk and Novosibirsk. We assessed clinical data: level of pain syndrome – using visual analog scale (VAS), neck disability index (NDI), Oswestry disability index (ODI), MacNab and Nurick clinical outcomes, as well as the results of plain spine radiography and neuroimaging studies (MRI, MSCT). Multicenter study of the results of staging surgical procedures with the use of specialized microsurgical instruments in the treatment of patients with tandem stenosis of cervical and lumbar spine allowed us to confirm their high clinical and radiological effectiveness.
BONE MORPHOGENETIC PROTEIN-2 INFLUENCE ON METABOLIC ACTIVITY AND PROTEOGLYCAN SYNTHESIS BY INTERVERTEBRAL DISC CELLS
Authors: Белых  , Belykh Evgeniy , Бардонова  , Bardonova Lyudmila , Бывальцев  , Byvaltsev Vadim
Published: April 2017

Modern therapeutic strategies for intervertebral disc repair mainly focus on targeting molecular pathways of extracel-lular matrix degeneration. Anabolic strategies for regeneration are aimed to increase production of major extracellular molecules. Members of TGF-b superfamily proteins, particularly the bone morphogenetic proteins (BMP) have a high regenerative potential regarding the mesenchymal cells. The goal of this study is to study production of proteoglycans by the intervertebral disc cells under the influence of bone morphogenetic protein 2.Material and methods. The experiment was carried out on the cell cultures derived from the annulus fibrosis cells and nucleus pulposus cells of the human intervertebral disc. We studied cell livability, metabolic activity and proteoglycan expression. Cell livability was assessed using the trypan blue staining. Alamar blue test was used for the estimation of metabolic activity. Amount of sulfated glycosaminoglycans was assessed using the assay based on the reaction with 1,9-Dimethylmethylene Blue.Results. Cultivation with bone morphogenetic protein 2 in different concentrations did not decrease livability of the cells. Study cell cultures with application of bone morphogenetic protein 2 in different concentrations showed significant increase in metabolic activity and proteoglycan synthesis by the annulus fibrosis cells. Despite the relative increase in the number of the nucleus pulposus cells treated with the bone morphogenetic protein 2, the differences in metabolic and synthetic activity compared with control group was not significant. Conclusion. The bone morphogenetic protein 2 has an anabolic effect towards the intervertebral disc cells, particularly in the production of proteoglycans by the annulus fibrosis cells.
EFFECT OF INTRAOSSEOUS INTRODUCTION OF SELENIUM/ARABINOGALACTAN NANOGLYCOCONJUGATE ON THE MAIN INDICATORS OF PRIMARY METABOLISM IN CONSOLIDATION OF BONE FRACTURE
Authors: Родионова  , Rodionova Lyubov , Сухов  , Sukhov Boris , Самойлова  , Samoylova Liliya , Шурыгин  , Shurygin Mikhail , Шурыгина  , Shurygina Irina
Published: April 2017

The research was carried out on 9rabbits (males) of Chinchilla breed with modeling of standard perforating fracture of femoral bone. We performed intraosseous introduction of nanocomposite elemental selenium and heteropolysaccharide arabinogalactan 50mg Se/kg to individuals of experimental group (n=3) and NaCl 0,9% to individuals of control group (n=6). It was established that local intraosseous introduction of the nanocomposite with perforated fracture model did not affect basal metabolic indicators (body temperature, oxygen consumption, carbon dioxide production), but boosted the metabolic processes in the area of the surgical wound from the 9th to 21st days. It proves the bioavailability of the drug and the possibility of creating a local depot of selenium using a nanocomposite.
MODERN FEATURES OF VATS TREATMENT OF SPONTANEOUS PNEUMOTHORAX AS A COMPLICATION OF BULLOUS LUNG EMPHYSEMA: A SYSTEMATIC LITERATURE REVIEW
Authors: Бадмаев  , Badmaev Dymbryl , Будаев  , Budaev Anatoliy , Токтохоев  , Toktokhoev Vladimir , Чепурных  , Chepurnykh Elena
Published: April 2017

The article presents a literature review on the VATS treatment of spontaneous pneumothorax as a complication of bullous lung emphysema. The review considers questions of etiology, diagnostics and therapeutic tactics in VATS treat-ment of spontaneous pneumothorax with bullous emphysema. The main target of surgeons is not only elimination of spontaneous pneumothorax, but quick exposure of lung parenchyma pathology in order to predict possible reoccurrence of spontaneous pneumothorax. Video-assisted thoracoscopy in this case is a mini-invasive, less traumatic and highly efficient method of treatment of spontaneous pneumothorax. Anti-relapsing surgical interventions with pleurodesis are unanimously considered to be necessary. An integrated and differentiated approach to the treatment of spontane-ous pneumothorax as a result of bullous emphysema helps to determine diagnostic and therapeutic tactics of VATS treatment and choice of pleurodesis induction leading to improved treatment outcomes in this category of patients.
Predictors of exaggerated blood pressure response to aerobic exercise in obese adolescents
Authors: Рычкова  , Rychkova Lyubov , Погодина  , Pogodina Anna , Данилюк  , Danilyuk Lyubov , Машанская  , Mashanskaya Aleksandra
Published: April 2017

Association of blood pressure response during exercise of cardiometabolic risk factors (CMRF) is demonstrated in adults. The objective was to determine predictors of raised exercise blood pressure (BP) response during aerobic exercise in obese adolescents.The maximal treadmill test using the Bruce protocol was performed in 82 normotensive obese adolescents (12–17 y.o., 43 boys). Adolescents were divided into 2 groups: group 1 (n = 68) – children with normal BP response to exercise; group 2 (n = 14) – adolescents who had excessive increase in systolic BP (SBP) during exercise. We used logistic regression analysis to examine the associations between cardiometabolic risk factors (CMRF) and SBP response during exercise with adjustment for baseline SBP, sex, age and duration of exercise test period.We have found exaggerated BP response to exercise in 17.1 % of patients. In adolescents of group 2 baseline SBP was higher than in group 1 (p = 0.01). Glucose level and lipid metabolism disturbances, SDS Z-score, baseline SBP levels only by 24.8 % explained the formation of the hyperreactive SBP response to exercise (χ2 = 13.2; р = 0.068). Only baseline SBP was an independent predictor for exaggerated BP response (p = 0.025) and age was a significant trend towards influence (p = 0.054).Exaggerated BP response to exercise in normotensive obese adolescents is more common for older adolescents who have baseline SBP levels in the range of elevated normal values. Knowing CMRF as well as the degree of excess weight has no effect on excessive BP rise during exercise.
on some aspects of furosemide routine prescription at acute respiratory distress syndrome at the prehospital stage
Authors: Горбачёва  , Gorbacheva Svetlana , Дац  , Dats Andrey , Дац  , Dats Lyudmila , Сандаков  , Sandakov Pavel
Published: April 2017

We studied the effect of furosemide application at the pre-hospital stage on survival rate and mortality risk in patients with acute respiratory distress syndrome. It was found that out of 665 patients admitted to intensive care units, 90 have been diagnosed with acute respiratory distress syndrome, of which 75 have noted decreased preload. Those patients were divided into two groups: group 1 (n = 28) received furosemide on the first day, group 2 (n = 47) did not receive furosemide. The patients of both groups matched by age, the severity of the condition and the severity of organ dysfunction. The 10-day survival rate in the patients with acute respiratory distress syndrome and reduced preload received furosemide at the pre-hospital stage made 11 % and was significantly lower than in the patients without furosemide– 43 % (p = 0.031). The frequency of multiple organ dysfunction syndrome in group 1 the was statistically higher than in patients without furosemide (93 % and 75 % respectively; p = 0.048). Furosemide admin-istration in patients with acute respiratory distress syndrome and reduced preload 1.8 times increases the relative mortality risk (p = 0.032).
efficiency of various methods of injection of antituberculosis agents in young patients with destructive pulmonary tuberculosis
Authors: Гусева  , Guseva Valeriya , Собкин  , Sobkin Aleksandr , Мишина  , Mishina Anastasiya , Мишин  , Mishin Vladimir , Морозов  , Morozov Ilya , Петров  , Petrov Daniil
Published: April 2017

The article presents the data of the randomized clinical trial of 80 young patients with destructive pulmonary tuber-culosis for various methods of TB drugs injection. Particular attention is paid to the role of parenteral drug delivery. The work demonstrated that newly diagnosed patients with destructive pulmonary tuberculosis of younger age the most optimal and effective is parenteral injection of injectable forms of TB drugs, allowing to halt bacterioexcretion for 3 months in 92.5 % of cases and to close cavities in the lungs in 80 %. In newly diagnosed patients with destructive pulmonary tuberculosis of young age oral use of tablets and of TB drugs is not effective enough, allowing to achieve 3 months cessation of bacterial isolation in 85.2 % of cases and to close cavities in the lungs in 42.5 %. Undesirable side reactions to parenteral administration of injectable forms of TB drugs were detected in 17.5 % of patients and to sep-arate oral tablet forms – in 20 %, fatal reactions were observed in 5 % of cases of drug delivery regardless of method.
assessment of the relationship between the vaginal microecosystem in teenage girls with reproductive disorders
Authors: Гаращенко  , Garashchenko Nadezhda , Немченко  , Nemchenko Ulyana , Григорова  , Grigorova Ekaterina , Приставка  , Pristavka Aleksey , Джиоев  , Dzhioev Yuriy , Шабанова  , Shabanova Natalya , Белькова  , Belkova Natalya
Published: April 2017

Violations of indigenous microflora composition associates with a wide variety of gynecological compli-cations. Thus, the qualitative and quantitative composition of lactobacilli and their associative capacity and functional activity may serve as a criterion of microecological well-being in the body. Aim. To study species diversity of lactobacilli in vaginal biotope teenage girls with gynecological pathologies and to assess the extent of their relationship with the combination of diversity profiles associated opportunistic pathogens.Materials and methods. The study included 107 adolescents with reproductive system disorders. The study was con-ducted with the use of gynecological and general microbiological methods.Results. It was revealed that in vaginal biocenosis of the studied group of teenage girls dominating lactobacilli were Lactobacillus plantarum and L. crispatus, the incidence of other species did not exceed 21 %. Among the representa-tives of opportunistic pathogenic microflora dominated coccal microflora and Corynebacterium spp., being the part of normal flora of vaginal mucosa. Analysis of species composition revealed a statistically significant relationship between certain types of lactobacilli and opportunistic microorganisms, i.e. lactobacilli showed no antagonistic activity towards the opportunistic microorganisms, and formed symbiotic relationships with them.Conclusions. Most commonness was found among the minor species of lactobacilli (L. iners, L. gasseri, L. jensenii), coagulase-negative staphylococci and fungi of Candida genus, and that increases the risk of transformation of normal microflora in the pathological one.
BIoInfoRmatIonal analySIS of YersiniapseudotuberculosisIP32953 CRISPR/CaSSyStem
Authors: Степаненко  , Stepanenko Liliya , Парамонов  , Paramonov Aleksey , Колбасеева  , Kolbaseeva Olga , Воскресенская  , Voskresenskaya Ekaterina , Борисенко  , Borisenko Andrey , Перетолчина  , Peretolchina Nadezhda , Джиоев  , Dzhioev Yuriy , Злобин  , Zlobin Vladimir
Published: April 2017

The results of this study include Yersinia pseudotuberculosis CRISPR/Cas system structure analysis. CRISPR/Cas system is a specific adaptive protection against heterogeneous genetic elements. The object of research was the complete genome of Y. pseudotuberculosis IP32953 (NC_006155). CRISPR/Cas system screening was performed by program modelling methods MacSyFinder ver. 1.0.2. CRISPR loci screening and analyzing were carried out by program package: CRISPR Recognition tool (CRT), CRISPI: a CRISPR Interactive database, CRISPRFinder, and PilerCR. Spacer sequences were used in order to find protospacers in ACLAME, GenBank-Phage and RefSeq-Plasmid databases by BLASTn search algorithm. Protospacer sequences could be found in genomes of phages, plasmids and bacteria. In last case complete genomes of bacteria were analyzed by online-tool PHAST: PHAge Search Tool. Y. pseudotuberculosis IP329353 has CRISPR/Cas system that consists of one sequence of cas-genes and three loci. These loci are far away from each other. Locus YP1 is situated in close proximity to cas-genes. Protospacers were found in genomes of Y. pseudotuberculosis PB1/+, Y. intermedia Y228, Y. similis str. 228, Salmonella phage, Enterobacteria phage, Y. pseudotuberculosis IP32953 plasmid pYV and plasmid of Y. pseudotuberculosis IP31758. Thus, the combination of four program methods allows finding CRISPR/Cas system more precisely. Spacer sequences could be used for protospacer screening.
financial and economic aspects of access to health care in russia
Authors: Чубарова  , Chubarova Tatyana
Published: April 2017

The article deals with the financial and economic problems of access to health care for the citizens of Russia. It analyzes basic methodological approaches as well as the corresponding official statistics and sociological research on the state of the country’s health system, which, according to author’s opinion, can describe the situation with the accessibility of health care. It is shown that underfunding of health care from public sources and the associated expansion of private finances, can signal problems with access for Russian citizens. Especially if the overall socio-economic situation in the country is taken into account, namely the federal state policy of shifting health expenditures to regional level under the conditions of high regional social and economic differentiation and high income inequality. Thus, the development of fiscal policy within the budget federalism in Russia should take into account its implications for people’s access to health.
ormatIon of sustaIned antItumor effect In mIce’s lIver and spleen (cba × c57bl/6)f1 after a sIngle Intravenous InjectIon of zymosan-treated neutrophIls
Authors: Любимов  , Lyubimov Andrey , Любимов  , Lyubimov Gennadiy , Козлов  , Kozlov Vladimir
Published: April 2017

Application of zymosan in medicine is limited because of its side effects, since the native granules of zymosan directly activate serum complement components and phagocytic cells. In this regard, it was decided at the outset (ex vivo) to incorporate the granules of zymosan in the neutrophils, and only then inject these “loaded” zymosan neutrophils into the bloodstream. Our experiments showed a total inhibition of the weight accretion of both tumor affected liver (from 5220±963 to 1327±219mg) and spleen (from 328±29 to 187±64mg) of mice. Thus these data showed that even on the 17th day after a single intravenous injection zymosan-treated neutrophils persist expressed manifestations of anti-tumor activity in the liver and spleen, that may be basis for using this method for antimetastatic preventive measures in liver and other localizations
Influence of thePENTAPHYLLOIDES FRUTICOSA l.lInIment on tba-actIve products and antIoxIdant status In wound InjurIes assocIated wIth experImental dIabetes mellItus
Authors: Бальхаев  , Balkhaev Mikhail , Гармаев - , Garmaev Bato-Tsyren , Хитрихеев  , Khitrikheev Vladimir , Николаева  , Nikolaeva Irina
Published: April 2017

The aim of the work was to evaluate the influence of the liniment from Pentaphylloides fruticosa L. on lipid peroxidation in rats with experimental diabetes mellitus accompanied by wound process.The liniment was developed with the use of NanoDeBee30 device. Experiments were carried out on white male Wistar rats. Experimental diabetes mellitus was simulated by a single intraperitoneal introduction of alloxan in a dose of 100mg/kg. The skin injury was simulated by inflicting planar wounds 2.0×2.0cm in size. 5mg of the 5%-liniment from Pentaphylloides fruticosa was put on the wounds of the first experimental group once a day for 14 days. The sec-ond experimental group was treated by the preparation of comparison – balsamic liniment. The wounds of the control group were treated with glycerin-lanolin ointment. On the 7th, 14th and 21st day the concentration of TBA-active products in the blood serum was determined to estimate the intensity of lipid peroxidation processes; the contents of reduced glutathione and catalase activity were determined to evaluate the antioxidant status. The results of the experiments verify the marked curative effect of the liniment from Pentaphylloides fruticosa L. on the healing of the wounds on the background of experimental diabetes mellitus in rats
phylogeography of the Beijing lineage in mongolia
Authors: Огарков  , Ogarkov Oleg , Орлова  , Orlova Elizaveta , Жданова  , Zhdanova Svetlana , Бадлеева  , Badleeva Marina
Published: April 2017

Mongolia is one of the seven countries in Western Pacific regions with high burden of tuberculosis. The earlier research indicates that there is a difference in the distribution of some epidemiologically important subtypes of the Beijing lineage in Mongolia and adjacent Russian regions.Aim of the research: assessment of genotypic structure of M. tuberculosis (MBT) on the border of Russia and central regions of Mongolia.Materials and methods: The DNAs of 143 clinical isolates of MBT from Russian border (46.2%) and central (53.8%) regions of Mongolia have been genotyped by the 24-locus MIRU-VNTR and RD105/RD207. Strains of the Beijing lineage have been analyzed additionally according to the classification by Merker et al. (2015). Results. The study of MBT in Mongolia indicates significant predominance of strains of the Beijing lineage (79.0%) and Beijing MIT 17 subtype (72.6%). However, the strains of the Beijing subtype W148, widespread in Irkutsk Oblast and Buryatia, have not been noted in Mongolia. According to the classification by M.Merker et al., the majority of studied strains of the Beijing lineage (85.8%) relate to the clonal complexe CC4, infrequently detected in Russian border regions. Statistically significant differences between distribution of clonal complexes among border with Russia and central regions of Mongolia have not been detected.Conclusions. Strains of the clonal complex CC4 of Beijing lineage dominate in central and border to Russia regions of Mongolia, this allows assuming that the different geographical regions were sources of MBT strains, prevalent in Mongolia and adjacent Russian regions.