bluuebunny/crossref_metadata_2025 huggingface dataset:
Twenty-year longitudinal evaluation of blood-based DNA methylation biomarkers for early prediction of Alzheimer’s disease
Authors: Hackenhaar Fernanda Schäfer , Josefsson Maria , Adolfsson Annelie Nordin , Landfors Mattias , Kauppi Karolina , Porter Tenielle , Milicic Lidija , Laws Simon M. , Hultdin Magnus , Adolfsson Rolf , Degerman Sofie , Pudas Sara
Published: November 2022

Background: DNA methylation (DNAm) is an epigenetic mechanism reflecting both inherited and environmental influences, and is a promising biomarker of multifactorial aging-related disorders like Alzheimer’s disease (AD). Early prediction of AD is critical, but little is known about the time-course of DNAm biomarkers long before symptom onset.
Methods: The long-term predictive ability of four existing DNAm-based epigenetic age acceleration clocks was tested in a longitudinal case-control sample (50 late-onset AD cases; 51 age- and sex-matched controls) with prospective data up to 16 years prior to clinical onset (mean: 8 years), and a post-onset follow-up. In addition, novel blood-based DNAm biomarkers for AD prediction were generated with epigenome-wide longitudinal linear mixed effects models, as well as sparse partial least squares discriminant analysis applied at time-points 10-16 years pre-onset and 0-7 years post-onset.
Results: Epigenetic age acceleration clocks did not differentiate cases from controls at any point during the 20-year follow up time (ps>0.05). Our new DNA biomarkers, comprising 73, 7, and 27 CpG sites respectively, had excellent in-sample discriminatory and predictive accuracy on average 8 years prior to clinical onset (AUCs=71.1-98.2% including age, sex, and white blood cell proportions). The longitudinal panel of CpGs replicated nominally (p=0.012) in an external cohort (n=146 cases, 324 controls). However, compared with the established genetic marker APOEε4 our panel had a limited effect size (OR=1.38 per 1 SD panel score increase vs. OR=13.58 for ε4-allele carriage) and discriminatory accuracy in the external cohort (AUC=77.2% vs. 87.0% for models with age, sex, and white blood cell proportions). A literature review showed low overlap (n=4) across 3275 CpGs previously reported to be AD-associated in 8 published studies, and no overlap with our currently identified CpGs.
Conclusions: The results extend prior studies showing a limited predictive and prognostic value of epigenetic age acceleration in AD by considering a longer pre-onset follow-up time, and with appropriate control for age, sex, APOE, and white blood cell proportions. The findings further highlight challenges with replicating discriminatory or predictive CpGs across studies.
PARP14 inhibition restores PD-1 immune checkpoint inhibitor response following IFNγ-driven adaptive resistance
Authors: Wong Chun Wai , Evangelou Christos , Sefton Kieran , Leshem Rotem , Sergiou Kleita , Carro Macarena Fernandez , Uzuner Erez , Mole Holly , Telfer Brian , Wilcock Daniel , Smith Michael , Kunii Kaiko , Perl Nicholas , Lorigan Paul , Williams Kaye , Rao Patricia , Nagaraju Raghavendar , Niepel Mario , Hurlstone Adam
Published: November 2022

Adaptive resistance limits immune checkpoint blockade therapy (ICBT) response duration and magnitude. Interferon γ (IFNγ), a critical cytokine that promotes cellular immunity, also induces adaptive resistance to ICBT. Using syngeneic mouse tumour models, we confirmed that chronic IFNγ exposure confers resistance to anti-Programmed cell death protein 1 (α-PD-1) therapy. We identified consistent upregulation of poly-ADP ribosyl polymerase 14 (PARP14) in both chronic IFNγ-treated cancer cells and patient melanoma with elevated IFNG expression. Knockdown or pharmacological inhibition of PARP14 increased effector T cell infiltration into tumours derived from cells pre-treated with IFNγ and decreased the presence of regulatory T cells, leading to restoration of α-PD-1 sensitivity. Finally, we determined that tumours which spontaneously relapsed following α-PD-1 therapy could be re-sensitised upon receiving PARP14 inhibitor treatment, establishing PARP14 as an actionable target to reverse IFNγ-driven ICBT resistance.
AVA-Net: an open-source deep learning network for arterial-venous area segmentation in OCT angiography
Authors: Yao Xincheng , Abtahi Mansour , Le David , Ebrahimi Behrouz , Dadzie Albert , Lim Jennifer
Published: November 2022

Differential artery-vein (AV) analysis in optical coherence tomography angiography (OCTA) holds promise for early detection of eye diseases. However, currently available methods for AV analysis are limited for binary processing of retinal vasculature in OCTA, without quantitative information of vascular perfusion intensity. This study is to develop a deep learning network AVA-Net for automated AV area (AVA) segmentation in OCTA, and thus to enable quantitative AV analysis of vascular perfusion intensity. Seven new OCTA features, including arterial area (AA), venous area (VA), AVA ratio (AVAR), total perfusion intensity density (T-PID), arterial PID (A-PID), venous PID (V-PID), and arterial-venous PID ratio (AV-PIDR), have been developed and validated for early detection of diabetic retinopathy (DR). Each of these seven features was evaluated for quantitative comparison of OCTA images from healthy controls, diabetic patients without DR (NoDR), and mild DR. It was observed that the area features, i.e., AA, VA and AVAR, can reveal significant differences between the control and diabetic eyes (NoDR and mild DR), but cannot separate NoDR and mild DR from each other. Vascular perfusion parameters, including T-PID and V-PID, can differentiate mild DR from control and NoDR groups, but cannot separate control and NoDR from each other. In contrast, the AV-PIDR can disclose significant differences among all three groups, i.e., control, NoDR, and mild DR. The AVA-Net validated in this study is available on GitHub for open access.
Laparoscopic parenchyma-sparing resections for solid pseudopapillary tumors located in the head of pancreas
Authors: Zou Zhengdong , Feng Lu , Peng Bing , Liu Jianhua , Cai Yunqiang
Published: November 2022

Background
Solid pseudopapillary tumor (SPT) of pancreas is a rare low-grade malignant tumor. We aim to identify the safety and feasibility of laparoscopic parenchyma-sparing pancreatectomy for SPT located in the head of pancreas.
Methods
From July 2014 to February 2022, 62 patients with SPT located in the head of pancreas were operated laparoscopically in two institutions. These patients were divided into two groups basing on the operative strategy: laparoscopic parenchyma-sparing pancreatectomy (27 patients, group 1) and laparoscopic pancreaticoduodenectomy (35 patients, group 2). Data were retrospectively collected and analyzed in terms of demographic, peri-operative variables, and long-term follow-up outcomes.
Results
The demographic characteristics of patients in two groups were comparable. Compared with that in group 2, the patients in group 1 required fewer operative time (263.4 ± 37.2 min vs. 332.7 ± 55.6 min, p < 0.001), and suffered from less blood loss (105.1 ± 36. ml vs. 188.3 ± 150.7 ml, p < 0.001). No patients in group 1 suffered from tumor recurrence or metastases. However, 1 (2.5%) patient in group 2 suffered from liver metastases.
Conclusion
Laparoscopic parenchyma-sparing pancreatectomy is safe and feasible for SPT located in the head of pancreas, with favorable long-term functional and oncological results.
Electrocardiographic patterns of ventricular pre-excitation in the dogs with right-sided accessory pathways
Authors: Perego Manuela , Pariaut Romain , Lombardo Sergio Federico , Moise Sidney N , Cavallini Damiano , Santilli Roberto
Published: November 2022

Introduction: The aim of the study was to describe the electrocardiographic features of ventricular pre-excitation (VPE) patterns in dogs with right-sided accessory pathways.
Animals, materials and methods: Twenty-six dogs with confirmed accessory pathway (AP) via electrophysiological mapping were included in the study. The following parameters were determined: P-δQRS interval, δQRS duration, δQRS axis, δQRS morphology, δ-wave polarity, Q-wave, R-wave, R’-wave, S-wave amplitude and R/S ratio.
Results: The median δQRS complex axis in the frontal plane was: + 68° (IQR 52.5) for right anterior APs, - 24 ° (IQR 24) for right postero-septal APs, - 43.5 ° (IQR 27.25) for right posterior APs (P = 0.007). In lead I, R wave was overall taller in right posterior APs (P = 0.04) for differences among medians within groups, and R’ wave in lead II and in lead III was significantly taller in right anterior Aps (respectively P = 0.02 and P = 0.01) for differences among medians within groups. In precordial leads of all dogs, R/S was < 1 in V1 and > 1 in all leads from V2 to V6.
Surface electrocardiogram can be used to distinguish right anterior APs from right posterior and right postero-septal ahead of an invasive electrophysiological study.
Synthesis and characterization of Zn based bi metallic micro porous MOF composites for effective formaldehyde sensing at room temperature
Authors: Reddy A. Jagan Mohan , Nagaraju P. , Namratha E. , Babu M. S. Surendra
Published: November 2022

In the present work, we fabricated 1:1 bimetallic ZnCu(NA); ZnCd(NA); and ZnCo(NA) metal organic frameworks (MOFs) using nicotinic acid (NA) as organic linker by solvothermal method. Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, X-Ray Photoelectron Spectroscopy (XPS), and scanning electron microscopy (SEM) were used to describe all bimetallic MOF topologies. These compounds were used to develop chemiresistive sensor devices for sensing of formaldehyde vapor at room temperature. Drop-casting method was used to make the sensing device on a glass plate with silver electodes. The resulting bimetallic sensors exhibited excellent sensitivity, good linearity and more stability in the detection of formaldehyde at 1 to 50 ppm concentrations. ZnCo(NA) exhibited more response towards formaldehyde vapor than other bimetallic MOFs. Transient response analysis was used to determine response and recovery times, as well as stability over time. These findings prove the application prospects of bimetallic Zn/Cu, Zn/Cd and Zn/Co MOFs in the selective and sensitive detection of formaldehyde at room temperature.
R-phycocyanin from porphyra haitanensis attenuates apoptosis, ROS accumulation and cell cycle arrest induced by ameliorated cigarette smoke extract through AKT/MAPK/NF-κB signaling in human lung fbroblasts HFL1 cells
Authors: Feng Yanyu , Lu Hanjin , Zheng Baodong , Zhang Yi
Published: November 2022

The risk of human lung diseases and abnormal development under the toxic environmental exposure conditions of passive cigarette smoke is emerging. R-phycocyanin (R-PC) has health benefits for a range of lung diseases including lung injury. The goal of this study was to investigate R-PC improves cigarette smoke-induced cell apoptosis and cycle arrest and its underlying molecular mechanisms in human lung fibroblasts. The effect of R-PC on cytotoxicity, apoptosis, ΔΨm and cell cycle arrest were measured through LDH assay, Hoechst/PI staining, JC-1 assay, flow cytometry, ELISA. The mechanisms of R-PC used DCFH-DA fluorescent probe for reactive oxygen species (ROS) level and western blotting analysis for signaling. Results showed that R-PC have great anti-oxidant activity and can inhibit the generation of radicals, expressed a protective effect against CSE-induced apoptotic HFL1 cells death and cycle phase arrest. Furthermore, CSE added the level of ROS and activated p38 and JNK signaling, inhibited AKT and NF-κB signaling. Pre-treatment with R-PC inhibited CSE-induced p38, JNK phosphorylation and ROS production, activated AKT, ERK, MKK4 and NF-κB signaling pathways. The findings suggested that R-PC treatment ameliorated CSE-induced ROS accumulation, apoptosis and cell cycle arrest by AKT/MAPK/NF-κB signaling pathways in HFL1 cells.
Complete genome sequence of a tentative novel capillovirus isolated from Gerbera jamesonii
Authors: Bak Sangmin , Jeong Wonyoung , Kim Minhui , Lee Su-Heon , Kim Sung Tae , Lee Eunsook , Kim Chang-Kil , Kim San Yeong
Published: November 2022

The currently named gerbera virus A (GeVA) has been identified as a novel capillovirus with a complete genome of 6929 nucleotides (nt) (GenBank accession no. OM525829.1). GeVA was detected in Gerbera jamesonii using high-throughput RNA sequencing analysis. The complete genome represents a single linear RNA with two open reading frames (ORF). The large ORF encodes a polyprotein contains four domains, while the smaller ORF encodes a movement protein. The addition of find two UUAGGU promoters for subgenomic RNA (sgRNA) transcription was also identified in this study. BLAST analysis demonstrated that it shared the highest identity with the rubber tree capillovirus 1 (MN047299.1) (complete nucleotide: 68.54%; polyprotein amino acid: 44.53%). Phylogenetic analysis of complete genome and replication protein sequences place GeVA alongside other genus Capillovirus in the family Betaflexiviridae. These data suggest that GeVA is a new member of the genus Capillovirus.
Conventional versus Video-assisted Laryngoscopy for Perioperative Endotracheal Intubation (COVALENT): A Randomized, Controlled Multicenter Trial
Authors: Schmid Benedikt , Eckert Dominik , Meixner Andreas , Pistner Paul , Malzahn Uwe , Berberich Monika , Happel Oliver , Meybohm Patrick , Kranke Peter
Published: November 2022

Background:Data on the routine use of video-assisted laryngoscopy in peri-operative intubations are rather inconsistent and ambiguous, in part due to small populations and non-uniform outcome measures in past trials. Failed or prolonged intubation procedures are a reason for relevant morbidity and mortality. This study aims to determine whether video-assisted laryngoscopy (with both Macintosh-shaped and hyperangulated blades) is at least equal to the standard method of direct laryngoscopy with respect to the first-pass success rate. Furthermore, validated tools from the field of human factors will be applied to examine within-team communication and task load during this critical medical procedure.
Methods: In this randomized, controlled, three-armed parallel group design, multi-centre trial, a total of more than 2500 adult patients scheduled for perioperative endotracheal intubation will be randomized. In equally large arms, video-assisted laryngoscopy with a Macintosh-shaped or a hyperangulated blade will be compared to the standard of care (direct laryngoscopy with Macintosh blade). In a pre-defined hierarchical analysis, we will test the primary outcome for non-inferiority first. If this goal should be met, the design and projected statistical power also allow for subsequent testing for superiority of one of the interventions.
Various secondary outcomes will account for patient safety considerations as well human factors interactions within the provider team and will allow for further exploratory data analysis and hypothesis generation.
Discussion:This randomized controlled trial will provide a solid base of data in a field where reliable evidence is of major clinical importance. With thousands of endotracheal intubations performed every day in operating rooms around the world, every bit of performance improvement translates into increased patient safety and comfort and may eventually prevent significant burden of disease. Therefore, we feel confident that a large trial has the potential to considerably benefit patients and anaesthetists alike.
Trial registration: ClincalTrials.gov NCT05228288
Protocol version: 1.1, November 15, 2021
Comparing Individual-, Family-, and Community-Level Effects on the Oral Health of Preschool Children: A Multilevel Analysis of National Survey Data
Authors: Lin Ying-Chun , Huang Shun-Te , Yen Cheng-Wei , Huang Yung-Kai , Shieh Tzong-Ming , Hsueh Chi-Wei , Yao Hu-Lin , Ho Pei-Shan
Published: November 2022

Background
Early childhood is a critical stage for the prevention of dental caries. The prevalence of caries in preschool children is still high in Taiwan, where National Health Insurance covers 99% of the population. The effort to improve the oral health of preschool children should be based on conceptual model that encompasses more than individual-level factors. This study input nationwide survey data in a conceptual model to evaluate the effects of comprehensive factors related to the high prevalence of caries in preschool children.
Methods
This observation study examined factors related to the oral health of preschool children by employing a comprehensive multilevel model to analyse nationally representative data from the Taiwan Oral Health Survey of Preschool Children (TOHPC) 2017–2018. Individual-level, family-level and community-level contextual effects were evaluated through multilevel analysis in this study. The proportional change in variance (PCV) was used to compare the multilevel model with the null model and individual-level, family-level, and community-level context effects.
Results
The estimated deft index for preschool children was 1.34 (1.22–1.47) at age 3, 2.20 (2.08–2.32) at age 4, and 3.05 (2.93–3.18) at age 5. The overall prevalence of caries in preschool children in Taiwan was 34.27% (30.76%, 37.78%) at age 3, 51.67% (48.99%, 54.35%) at age 4, and 62.05% (59.66%, 64.44%) at age 5. The model that included the individual-, family-, and community-context levels exhibited the highest reduction of variance (PCV = 53.98%). The PCV was further reduced to 35.61% when only the level of accessibility to dental services for individuals, families, and the community was considered. For the model in which no community-context cofactors were considered and the model considering only the individual level, the PCVs were 20.37% and 5.52%, respectively.
Conclusions
The most notable finding of this study is that to improve the oral health of preschool children, community-level factors should be targeted. Such community-based programs would effectively improve the oral health knowledge and behaviours of the parents and caregivers of preschool children. We suggest training more professional oral health educators to provide more community-based oral health promotion campaigns.
Spatial nonuniformity of landslide dam deposition and its quantitative characterization
Authors: Li Xing , Chen Qun , Liu Zhaozhao , Chen Chen
Published: November 2022

Landslide dams are formed by rapidly depositing landslide bodies with a loose structure and poor stability. The strong spatial nonuniformity of dam soil is a key factor for study on the seepage stability of landslide dams. In this paper, a 500×500×500 mm cubic model box was made and the dam soil was deposited in it by different sliding modes to investigate the nonuniformity of the deposition. Based on the basic principle of grading entropy, the distribution characteristics of the soil in different depositing zones using the entropy parameters A-B coordinates were analyzed. Finally, an index describing the degree of nonuniformity Nd was proposed to assess the deposition quantitatively. It was found that with the increasing sliding angle, the deposition showed prominent sorting characteristics in the sliding direction. The coarser and finer particles were mainly concentrated in the front and back parts of the deposition, respectively. Grading entropy has the advantage of being meticulous compared with traditional characteristic parameters. In the entropy parameters A-B coordinates, the points for the expected uniform deposition are more concentrated. And the points of different zones for the sliding deposition are arch-shaped in this coordinates. The Nd of the expected uniform deposition is only 0.057. And that of the deposition made by the sliding angle of 60° is the largest of 0.173. The research results can provide references for studying the spatial nonuniformity of landslide dam deposition.
COVID-19 in pregnancy influences the progression of labor: A single-institutional and observational study in Japan
Authors: Furuya Kiichiro , Tanaka Yoshitomo , Komatsu Naoto , Hirose Rikuto , Nakamae Akiko , Takemoto Yuki , Kurahashi Hiroki , Hayashida Harue , Fujiwara Sho , Yamashita Saya , Chang Yangsil , Shikado Kayoko , Tsubouchi Hiroaki , Seki Masayuki , Yamato Masaya , Ogita Kazuhide
Published: November 2022

The management of vaginal delivery during the COVID-19 pandemic remains unclear due to lack of information on labor characteristics. We aimed to clarify the effects of COVID-19 on the progression of vaginal labor.
The single-institutional and observational study of COVID-19 women in pregnancy (COVID-19 group, n = 50) delivering from 2020 to 2022, was conducted. Patients’ backgrounds, perinatal outcomes, labor characteristics (labor time and Bishop score), and the details of maternal COVID-19 were analyzed and compared with non-COVID-19 pregnant women (control group, n = 258).
The COVID-19 group showed rapidly progressing labor without adverse effect. The 1st and 2nd stage of labor time, and labor time after rupture of membrane were significantly shorter in the COVID-19 group than in the control group (P < 0.01; P < 0.01; and P < 0.05, respectively). The COVID-19 group had a higher Bishop score on initiation of labor (P < 0.01). Precipitous delivery in the COVID-19 group also significantly increased (P < 0.05). These results indicated that COVID-19 in pregnancy can affect the progression of labor rapidly.
This is the first report focusing on the characteristics of labor progression in COVID-19 women, indicating that COVID-19 can affect the progression of labor without complication.
Use of a real-time PCR method to quantify the primary infection of Plasmopara viticola in commercial vineyards in northwestern China
Authors: Yang Lujia , Chu Bingyao , Yuan Kai , Deng Jie , Jiang Caige , Ma Zhanhong
Published: November 2022

Grapevine downy mildew (GDM), caused by Plasmopara viticola (P. viticola), is a frequent disease of wine grapes in Ningxia Hui Autonomous Region (Ningxia), Northwestern China. At present, however, the primary infection of pathogens in this region has not been deeply investigated. The current study aims to better understand the epidemiological role of overwintering P. viticola and the levels of latent infection before GDM occurrence. We used a previously developed multiplex real-time PCR assay to quantify P. viticola in soil, leaf residue, and asymptomatic leaf samples from ten commercial vineyards in two consecutive years. We found that 60%, 80%, and 100% of the asymptomatic leaves, leaf residues, and soil samples were specific to P. viticola. The molecular disease index (MDI) was used to evaluate the levels of latent infection. Asymptomatic leaves with different levels of P. viticola infection during the latent period and high cumulative MDI correlated with a high area under the disease progress curve in terms of disease index (AUDPC-DI), and the correlation relationship between them was significant at P < 0.01. A well-correlated relationship was found between the AUDPC-DI in the previous year and the MDI data of leaf residues and asymptomatic leaves in the following year, as well as the AUDPC-DI, with corresponding correlation coefficients of 0.504, 0.498, and 0.595, respectively (P < 0.01). The findings indicate that the real-time PCR assay was a useful tool to accurately quantify the primary infection of P. viticola and to efficiently estimate the primary inoculum potentials of GDM epidemics in commercial vineyards.
Interrupted time series analysis using autoregressive integrated moving average (ARIMA) models: Effects of air pollution control policies on intracerebral hemorrhage mortality in Tianjin, China
Authors: Xu Jiahui , Yin Xiaolin , Jiang Tingting , Wang Shiyu , Wang Dezheng
Published: November 2022

Background: This research aims to quantitively evaluate the associations between air pollution control policies in Beijing-Tianjin-Hebei region and intracerebral hemorrhage (ICH) deaths in Tianjin and provide scientific evidence for the prevention and control of ICH as well as the management of air pollution.
Methods: This study used an interrupted time series analysis. Based on the data of ICH death cases in Tianjin collected by the Tianjin Center for Disease Control and Prevention, Autoregressive integrated moving average models (ARIMA) were fitted to assess the changes in ICH deaths before and after the interventions of air pollution control policies.
Results: There were 63,944 ICH deaths in Tianjin from 2009 to 2020, with an overall decreasing trend in ICH mortality. The secular trend change of the intervention in June 2014 was statistically significant (p = 0.03), with 0.69 (1.30 to 0.07) fewer ICH deaths per month. The immediate change of the intervention in October 2017 was statistically significant (p = 0.04), with 25.74 (50.62 to 0.85) fewer deaths from ICH in the month. A statistically significant immediate change in the intervention in December 2017 (p = 0.04), with 26.58 (-52.02 to -1.14) fewer deaths from ICH in the month. Moreover, a statistically significant immediate change in the intervention in March 2018 (p = 0.02), the number of ICH deaths decreased by 30.40 (56.41 to 4.40) in the month. After the interventions, an estimated 5984.76 ICH deaths (8.56%) were prevented.
Conclusion: The interventions of air pollution control policies during the study period were significantly associated with the reductions in ICH deaths in Tianjin. Surviving ICH patients and women were more sensitive to the protective effects of the interventions. Public health gains can be achieved by implementing interventions of air pollution control policy in cities with high levels of air pollution.
Horse Chestnut Tree Genome Reveals the evolutionary mechanism of Aescin and Aesculin biosynthesis
Authors: Sun Wei , Yin Qinggang , Wan Huihua , Xiong Chao , Xie Chong , Meng Xiangxiao , Wang Caixia , Gao Ranran , Chen Weiqiang , Xie Ziyan , Xue Zheyong , Yao Hui , Sun Peng , Xie Xuehua , Hu Zhigang , Nelson David , Xu Zhichao , Sun Xinxiao , Chen Shilin
Published: November 2022

Medicinal trees provide a main resource for diverse medicinal compounds. However, the biosynthesis of tree metabolites and their pathway evolution has gained limited understanding. Horse chestnut (Aesculus chinensis) is an important medicinal tree and its seeds are rich in aescins, barrigenol-type triterpenoid saponins (BAT), and aesculin, a coumarin glycoside, which are effective in the therapy of chronic venous insufficiency and asthenopia (eye strain). To understand the biosynthesis of these compounds, herein, we assembled a 470.04-Mb high-quality horse chestnut genome and characterized an Aesculus-specific whole-genome duplication (WGD) event. Spatial metabolome imaging, co-expression, and biosynthetic gene cluster analyses indicated that the Aesculus-specific WGD event led to the formation of two gene clusters (BGCs) including oxidosqualene cyclase, cytochrome P450 monooxygenase, cellulose synthase-derived glycotransferases, and BADH acyltransferases. Further biochemical investigation revealed the roles of AcOCS6, AcCYP716A278, AcCYP716A275, AcCSL1, and AcBAHD3 genes distributed between these two BGCs in catalyzing the formation of aescins. To understand the evolution of BAT pathways, the collinearity analysis showed the collinear BGC segments could be traced back to early-diverging angiosperms, then the essential gene-encoding enzymes necessary for the BAT biosynthesis were recruited before the split of Aesculus, Acer, and Xanthoceras. Meanwhile, we identified three UDP-glucosyltransferases and demonstrated their involvement in the biosynthesis of aesculin via a de novo synthesis. Taken together, these findings provide important information in understanding the evolution of gene clusters associated with medicinal tree metabolites.
Assessment of the Compressive Mechanical Behaviour of Injection Molded E-Glass/Polypropylene by Mechanical Testing and X-Ray Computed Tomography
Authors: Stamopoulos Antonios , Gazza Francesco , Neirotti Giuseppe
Published: November 2022

Nowadays, thermoplastic composite materials are considered among the most promising ones as they are gaining the interest of many industrial sectors due to their eco-friendly features such as their recyclability and their low-cost demand while being produced in high volumes. Especially in the automotive industry, there have always been used injection molded thermoplastics reinforced with glass fibers. Among the most concerning issues regarding the injection molded, short fiber-reinforced composites are the fiber distribution, the fiber orientation, and their effect on the overall mechanical performance. In the present work the effect of the injection molding on the compressive properties is investigated experimentally. To this context, compressive mechanical tests have been conducted in a range between sub-zero and elevated temperatures. In addition, X-Ray computed tomography is implemented for characterizing some crucial features. Among them, the fiber volume fraction, in local and global scale, and the orientation of the fibers through the thickness and length of the material are investigated.
Multi-objective integrated optimization study of Prefabricated building projects introducing sustainable levels
Authors: Peng Junlong , Feng Yue , Zhang Qi , Liu Xiangjun
Published: November 2022

As construction becomes greener, people have higher and higher requirements for engineering project management, which
makes it necessary to deeply study the comprehensive optimization of schedule, cost and sustainability level. Adhering to
the concept of low carbon and green, the article takes carbon emission factor into the total cost of building construction and
improves the traditional cost objective of engineering projects; then quantitatively analyzes the economic, environmental
and social impacts of assembled buildings from the perspective of sustainability, and introduces the sustainability objective
into the traditional duration-cost problem study, taking the duration of each job in the double code arrow diagram as the
independent variable to construct the duration -cost-sustainability level multi-objective optimization model. In order to solve the
type effectively, a series of Pareto optimal solutions are obtained using the NSGA-II algorithm, and the efficacy coefficient
method is used for program decision making. The results show that the Pareto solution set can provide effective support for the
project manager’s decision making, and the NSGA-II algorithm is effective in solving the multi-objective optimization model.
Continuous Symmetry Breaking in a Trapped-Ion Spin Chain
Authors: Monroe Christopher , Feng Lei , Katz Or , Haack Casey , Maghrebi Mohammad , Gorshkov Alexey , Gong Zhexuan , Cetina Marko
Published: November 2022

One-dimensional systems exhibiting a continuous symmetry can host quantum phases of matter with true long-range order only in the presence of sufficiently long-range interactions. In most physical systems, however, the interactions are short-ranged, hindering the emergence of such phases in one dimension. Here we use a one-dimensional trapped-ion quantum simulator to prepare states with long-range spin order that extends over the system size of up to $23$ spins and is characteristic of the continuous symmetry-breaking phase of matter. Our preparation relies on simultaneous control over an array of tightly focused individual-addressing laser beams, generating long-range spin-spin interactions. We also observe a disordered phase with frustrated correlations. We further study the phases at different ranges of interaction and the out-of-equilibrium response to symmetry-breaking perturbations. This work opens an avenue to study new quantum phases and out-of-equilibrium dynamics in low-dimensional systems.
First-principles calculations of equilibrium Ga isotope fractionations between several important Ga-bearing minerals and aqueous solutions
Authors: Zhang Jixi
Published: November 2022

This study predicts the equilibrium isotope fractionation factors for some important Ga-bearing species, including major minerals, aqueous solutions and gas phase systems. Equilibrium isotope fractionations of Ga are investigated by using first-principles quantum chemistry method at B3LYP/6-311 + G(d) level. The 1000ln(RPFR)s of Orthoclase, Albite, Quartz, Kaolinite, Forsterite, Montmorillonite, Gibbsite, Cassiterite, Aragonite, Sphalerite and Calcite are calculated with the method of the volume variable cluster model (VVCM). The 1000ln(RPFR)s of these minerals decrease in the order of Orthoclase > Albite > Quartz > Kaolinite > Forsterite > Montmorillonite > Gibbsite > Cassiterite > Aragonite > Sphalerite > Calcite. The solvation effect of Ga3+-bearing aqueous species is modeled by the water-droplet method and the 1000ln(RPFR)s of Ga3+-bearing aqueous species decrease in the order of [Ga(OH)4]−>[Ga(OH)3]>[Ga(OH)]2+>[Ga(OH)2]+>[Ga(H2O)6]3+. The calculation results show that equilibrium isotope fractionations of Ga between different minerals, solutions and gas phases are appreciable. Among minerals, Ga isotope fractionation has the largest value between Orthoclase and Calcite. Ga isotopic fractionation factor between these two minerals can reach 3.18 per mil at 100°C. Ga isotope fractionation between Ga-bearing aqueous species and minerals are important to give some information about the different geochemistry processes such as surficial geochemistry. This study has provided those important Ga isotope fractionation factors.
Test conditions of texture profile analysis for frozen dough
Authors: Dou Xinlai , Ren Xuyang , He Yinyuan , Liu Linlin , Zhang Guang , Sun Ying , Zhang Na , Chen Fenglian , Yang Chunhua
Published: November 2022

Wheat flour is more important for making frozen dough, this paper firstly conducted basic property of six wheat flour from different place and found BDHhgwf is more suitable to make frozen dough. Basic on it, texture profile analysis (TPA) has been put into applications in dough-made food industry, while the effects of the texture analysis parameters on properties of frozen dough were firstly investigated in this study. The results showed during TPA determination process of frozen dough, the variation of test parameters have an influence on the final result to some extent. The use of texture profile analysis (TPA) on frozen dough is addressed. The recommended test conditions are: pre-test speed 3.0 mm/s, test speed 1.0 mm/s, post-test speed 1.0 mm/s, and compression ratio 50%.
Study on the anode catalytic performance of graphenepolyaniline composites on direct methanol fuel cells
Authors: Wang Yixue , Qin Qi , He Fang , Chen Jing , Wu Changze
Published: November 2022

The aim of this study is to develop a noble metal dispersion-free anodic catalyst for one-step electrochemical synthesis of graphene/polyaniline (GP) composites using a mixture of graphene suspension and aniline monomer as the precursor by the potentiostat method. UV-vis (UV-VIS) spectroscopy and FT-IR (FT-IR) spectroscopy were used to characterize the structure of polyaniline (PANI) and GP. Cyclic voltammetry (CV) was used to test the electrochemical properties and electrocatalytic properties of polyaniline (PANI) and GP for methanol oxidation. The characterization results proved that the GP composite was successfully synthesized. The CV test curve showed that the electrochemical performance of the GP composite was significantly higher than that of PANI, with a larger specific capacity, which proved that the synergistic effect of the two composites was better played by the composite. However, the catalytic performance test for methanol oxidation showed that. The graphene/polyaniline (GP) composite has little catalytic effect on methanol oxidation.
Research on functional recovery training of adolescents based on the influence of balance force
Authors: Yang Xiwen
Published: November 2022

Dynamic balance is particularly important for maintaining, transforming and re-stabilizing various postures of the human body. In the process of sports, athletes need to have the ability to move quickly and continuously high intensity confrontation. Combined with the characteristics of adolescent physical function development, this paper aims at the problem that adolescent athletes have poor dynamic balance ability and cannot maintain the stability of the body center of gravity in the process of fast multi-direction motion and braking. Through the Y-Balance test and FMS test of 30 football players, the paired sample T test was carried out by the control experiment to compare whether there were significant differences in test indexes between the experimental group and the control group before and after the experiment. Independent sample t-test was conducted on the test indexes of the experimental group and the control group before and after the experiment to explore the influence of balance training on the physical function of adolescents.
Results: (1) the balance ability of the experimental group improved more than that of the control group, and there was a significant difference;(2) There is a significant difference in the total score of FMS test in the experimental group before and after the experiment (P = 0.01), while there is no significant difference in the total score of FMS test in the control group before and after the experiment.
Conclusion: Enhanced balance training can significantly improve the balance ability of adolescent athletes, help to improve the basic movement pattern,and it is of great significance for developing basic joint flexibility and stability and strengthening weak chain muscles.
Allogenic and Autologous anti-CD7 CAR-T cell Therapies in Relapsed or Refractory T-Cell Malignancies
Authors: Zhang Yinqiang , Li Chenggong , Du Mengyi , Jiang Huiwen , Luo Wenjing , Tang Lu , Kang Yun , Xu Jia , Wu Zhuolin , Wang Xindi , Huang Zhongpei , Wu Di , Chang Alex , Hu Yu , Mei Heng
Published: November 2022

Chimeric antigen receptor-T (CAR-T) therapy remains to be investigated in T-cell malignancies. CD7 is an ideal target for T-cell malignancies but is also expressed on normal T cells, which may cause CAR-T cell fratricide. Donor-derived anti-CD7 CAR-T cells using endoplasmic reticulum retention have shown efficacy in patients with T-cell acute lymphoblastic leukemia (ALL). Here we launched a phase I trial to explore differences between autologous and allogeneic anti-CD7 CAR-T therapies in T-cell ALL and lymphoma. Ten patients were treated and 5 received autologous CAR-T therapies. No dose-limiting toxicity or neurotoxicity was observed. Grade 1–2 cytokine release syndrome occurred in 7 patients, and grade 3 in 1 patient. Grade 1–2 graft-versus-host diseases were observed in 2 patients. Seven patients had bone marrow infiltration, and 100% of them achieved complete remission with negative minimal residual disease within one month. Two-fifths of patients achieved extramedullary or extranodular remission. The median follow-up was 6 (range, 2.7–14) months and bridging transplantation was not administrated. Patients treated with allogeneic CAR-T cells had higher remission rate, less recurrence and more durable CAR-T survival than those receiving autologous products. Allogeneic CAR-T cells appeared to be a better option for patients with T-cell malignancies.
Magnesium deficiency stress in rice can be alleviated by partial nitrate nutrition supply
Authors: Deng Na , Shang Qingyin , Zhu Hongyan , Gong Shidao , Qian Beibei , Yang Xiuxia
Published: November 2022

Magnesium (Mg) plays an imperative role in determining crop growth and development, but Mg-deficiency becomes more common, more severe and more widespread in farmland. The major source of nitrogen for rice is ammonium (NH4+). However, excessive application of NH4+ could inhibit Mg2+ uptake by rice. It has been accepted that partial nitrate (NO3-) nutrition may not only improve nitrogen (N) assimilation and rice growth, but also promote Mg2+ absorption. Nonetheless, the mechanisms underlying Mg and N interaction are not well documented. To explore the relationship between them in rice plant, a hydroponic experiment was conducted with different Mg (0.01, 1.00, and 5.00 mM) concentrations and various NO3-/NH4+ ratios (0/100, 25/75 and 50/50, %) under greenhouse conditions. Results showed that Mg-deficiency (0.01 mM) can cause yellow leaves, dwarf plants and fewer tillers during rice growth. Furthermore, Mg-deficiency treatment induced a major decrease in dry weight, root morphology and activity, photosynthetic properties, and Mg and N accumulation, while increase in antioxidant enzyme (MDA, SOD, POD and CAT) activities in rice, when compared with the high Mg (5.00 mM) or normal Mg (1.00 mM) treatment. The treatments with different NO3-/NH4+ ratios had no significant effect on these antioxidant enzyme activities in most cases under high or normal Mg conditions. However, under Mg-deficiency stress, the supply of partial NO3- lead to a significant drop in antioxidant enzyme activities. Moreover, partial NO3- supply significantly improved Pn, Tr, Gs and Ci under Mg-deficiency conditions. In particular, under Mg-deficiency stress, the supply of partial NO3- dramatically promoted the growth of the root system, boosted the occurrence of lateral roots, enhanced root vitality. Additionally, the supply of partial NO3- lead to significant increases of dry weight and N and Mg accumulation under the stress of Mg-deficiency. The results of this study suggested that the symptoms of Mg-deficiency stress in rice can be alleviated by partial NO3- supply.
Mechanistic Insights of NAC1 Nuclear Export and Its Role in Ovarian Cancer Resistance to Docetaxel
Authors: Zhang Yi , Dong ShunLi , Wang Xiao-Hui , Yang ShuMin , Guo FanFan , Zhang JingJing , Ji Cheng , Shi LiangRong , Cheng Yan , Hu Yan-Wei , Li ZhenYun , Peng Lei , Guo Ling-Chuan , Zhu Wei-Dong , Ren XC , Yang Jin-Ming
Published: November 2022

In this study, we uncovered the nuclear export of nucleus accumbens-associated protein-1 (NAC1) as a novel mechanism involved in ovarian cancer resistance to taxanes, the chemotherapeutic drugs commonly used in treatment of this malignancy. We showed that NAC1, a nuclear factor of the BTB/POZ gene family, has a nuclear export signal (NES) at the N terminus (aa 17-28), and this NES critically contributes to the NAC1 nuclear-cytoplasmic shuttling when tumor cells were treated with docetaxel. Mechanistically, the nuclear-exported NAC1 bound to cullin3 (Cul3) and cyclin B1 via its BTB and BOZ domains respectively, and the cyto-NAC1-Cul3 E3 ubiquitin ligase complex promotes the ubiquitination and degradation of cyclin B1, thereby facilitating mitotic exit and leading to cellular resistance to docetaxel. We also showed in in vitro and in vivo experiments that TP-CH-1178, a membrane-permeable polypeptide against the NAC1 NES motif, blocked the nuclear export of NAC1, interfered with the degradation of cyclin B1 and sensitized ovarian cancer cells to docetaxel. This study not only reveals a novel mechanism by which the NAC1 nuclear export is regulated and cyclin B1 degradation and mitotic exit are impacted by the NAC1-Cul3 complex, but also provides the nuclear-export pathway of NAC1 as a potential target for modulating taxanes resistance in ovarian cancer and other malignancies.
Perceived social support and posttraumatic growth among couples coping with gynecological cancer: A cross-sectional survey
Authors: Zhou Lihua , Stenmarker Margaretha , Henricson Maria , Li Ziye , Dai Yu , Hong Jingfang , Browall Maria
Published: November 2022

Background
Perceived social support was considered to be an essential resource of individual’s positive consequences (e.g., posttraumatic growth, PTG) during times of stressful conditions. However, there is no evidence on the relationship between perceived social support and PTG at the couple-based level in cancer research. This study aimed to explore the relationship between perceived social support and posttraumatic growth among couples coping with gynecological cancer.
Methods
A cross-sectional survey was conducted in China, from May 2019 to March 2020 in two comprehensive hospitals. A total of 126 couples, consisting of women diagnosed with gynecological cancer and their spouses, participated. The Multidimensional Scale of Perceived Social Support (MSPSS) and the Posttraumatic Growth Inventory (PTGI) were used to collect data. Paired t-tests, pearson’s correlations and actor-partner interdependence model (APIM) were used to analyze data.
Results
The actor effects of perceived social support on posttraumatic growth were supported (r = 0.419, p < 0.001; r = 0.467, p < 0.001); however, the partner effects of perceived social support on posttraumatic growth were not supported (r = -0.119, p༞0.05; r = 0.130, p༞0.05). Perceived social support was positively correlated between women diagnosed with gynecological cancer and their spouses (r = 0.244, p < 0.01). Furthermore, spouses’ perceived social support was positively correlated with women’s posttraumatic growth (r = 0.213, p < 0.05).
Conclusions
The actor effects of perceived social support on posttraumatic growth in couples coping with GC are supported. The findings also highlight that spouses’ perceived social support are positively correlated with women’s perceived social support and women’s PTG. Healthcare professionals should provide couple-based interventions that would increase couples’ identification and use of effective social support, and how to promote their PTG should be focused.
Clinical utilization of blinatumomab in Chinese monocentric patients with relapsed or refractory B-cell acute lymphoblastic leukemia
Authors: Zhu Jingkui , Fan Jixin , Xie Tiantian , Zhao Haiqiu , Lu Runqing , Zhang Yinyin , Li Yingmei , Xie Xinsheng , Wan Dingming , Jiang Zhongxing , Song Yongping , He Fei , Guo Rong
Published: November 2022

Purpose: Blinatumomab has promising applications in treating relapsed or refractory B-cell acute lymphoblastic leukemia (R/R B-ALL). However, there are few studies on using blinatumomab for treating R/R B-ALL patients in China. Therefore, we evaluated the efficacy and safety of blinatumomab among Chinese R/R B-ALL patients.
Methods: In total, 39 R/R B-ALL patients who received blinatumomab between October 2021 and August 2022 were selected as the study subjects. The primary endpoints include overall response rate (ORR) and complete minimal residual disease (MRD) response. Secondary endpoints included overall survival (OS) and adverse events (AEs).
Results: The ORR and OS for 19 patients with less than 5% bone marrow (BM) blasts were 63.2% and 11 months (7.3–14.7), respectively. The median OS for the 20 patients was unavailable, and 17 (or 85%) had a full MRD response. Twenty-seven patients (69.2%) reported having at least 1 AE. Hematologic toxicity and infections were the most common AEs. Allogeneic hematopoietic stem cell transplantation (allo-HSCT) was performed on nine patients who had been remission-free for a long time. In the first month, one patient (11.1%) developed thrombotic microangiopathy associated with HSCT (TA-TMA) following transplantation.
Conclusion: In R/R B-ALL patients, blinatumomab is an effective option in China.