bluuebunny/crossref_metadata_2025 huggingface dataset:
Bacillus coagulans prevents the decline in average daily feed intake in young piglets infected with enterotoxigenic Escherichia coli K88 by reducing intestinal injury and regulating the gut microbiota
Authors: Zhang Yanyan , Tian Xiaorong , Dong Yi , Li Rui , Shen Meng , Yi Dan , Wu Tao , Wang Lei , Zhao Di , Hou Yongqing
Published: November 2023

Background

EnterotoxigenicEscherichia coli(ETEC), an important intestinal pathogen, poses a significant threat to the intestinal health of piglets.Bacillus coagulans(BC), a potential feed additive, can improve the intestinal function of piglets. However, the effects of BC on growth performance and intestinal function in ETEC-infected piglets are still unclear. In this study, 24 7-day-old piglets were randomly assigned to three treatment groups: control group (fed a basal diet), ETEC group (fed a basal diet and challenged with ETEC K88) and BC+ETEC group (fed a basal diet, orally administered BC, challenged with ETEC K88). During Days 1-6 of the trial, piglets in the BC+ETEC group were orally administered BC (1×108CFU/kg). On Day 5 of the trial, piglets in the ETEC and BC+ETEC groups were orally administered ETEC K88 (5×109CFU/piglet). Blood, intestinal tissue, and content samples were collected from the piglets on Day 7 of the trial.

Results

The average daily feed intake in the ETEC group was significantly reduced compared to that of the control group. Further research revealed that ETEC infection significantly damaged the structure of the small intestine. Compared to the control group, the villus height and surface area of the jejunum, the ratio of villus height to crypt depth in the duodenum and jejunum, and the activities of catalase and total superoxide dismutase in the jejunum were significantly reduced. Additionally, the levels of myeloperoxidase in the jejunum, malondialdehyde in the plasma and jejunum, and intestinal epithelial apoptosis were significantly increased in the ETEC group. However, BC supplementation had significantly mitigated these negative effects in the BC+ETEC group by Day 7 of the trial. Moreover, BC supplementation improved the gut microbiota imbalance by reversing the decreased numbers ofEnterococcus,ClostridiumandLactobacillusin jejunum andEscherichia coli,BifidobacteriumandLactobacillusin the colon, as well as the increased number ofEscherichia coliin the jejunum induced by ETEC K88.

Conclusions

Overall, BC supplementation reduced the decline in average daily feed intake in ETEC K88-infected piglets by attenuating intestinal epithelial apoptosis and oxidative stress and regulating the gut microbiota. This suggests that BC may be used to prevent intestinal infections caused by ETEC in piglets.
Revisiting bacterial cytolethal distending toxin structure and function
Authors: Chen Henry , Ang Claire J. , Crowder Molly K. , Brieher William M. , Blanke Steven R.
Published: November 2023

Cytolethal distending toxins (CDTs) are intracellular-acting bacterial genotoxins generated by a diverse group of mucocutaneous human pathogens. CDTs must successfully bind to the plasma membrane of host cells in order to exert their modulatory effects. Maximal toxin activity requires all three toxin subunits, CdtA, CdtB, and CdtC, which, based primarily on high-resolution structural data, are believed to preassemble into a tripartite complex necessary for toxin activity. However, biologically active toxin has not been experimentally demonstrated to require assembly of the three subunits into a heterotrimer. Here, we experimentally compared concentration-dependent subunit interactions and toxin cellular activity of the Campylobacter jejuni CDT (Cj-CDT). Co-immunoprecipitation and dialysis retention experiments provided evidence for the presence of heterotrimeric toxin complexes, but only at concentrations of Cj-CdtA, Cj-CdtB, and Cj-CdtC several logs higher than required for Cj-CDT-mediated arrest of the host cell cycle at the G2/M interface, which is triggered by the endonuclease activity associated with the catalytic Cj-CdtB subunit. Microscale thermophoresis confirmed that Cj-CDT subunit interactions occur with low affinity. Collectively, our data suggest that at the lowest concentrations of toxin sufficient for arrest of cell cycle progression, mixtures of Cj-CdtA, Cj-CdtB, and Cj-CdtC consist primarily of non-interacting, subunit monomers. The lack of congruence between toxin tripartite structure and cellular activity suggests that the widely accepted model that CDTs principally intoxicate host cells as preassembled heterotrimeric structures should be revisited.
A hackathon as a tool to enhance research and practice on electronic health record systems’ interoperability for chronic disease management and prevention
Authors: Rigas Emmanouil S. , Kostomanolakis Stavros , Kyriakoulakos Nikolaos , Kounalakis Dimitrios , Petrakis Ioannis , Berler Alexander , Boumpaki Asimina , Karanikas Haralampos , Kelepouris Athanasios , Bamidis Panagiotis D. , Katehakis Dimitrios G.
Published: November 2023

Objectives

The development of a standardized technical framework for exchanging electronic health records is widely recognized as a challenging endeavor that necessitates appropriate technological, semantic, organizational, and legal interventions to support the continuity of health and care. In this context, this study delineates a pan-European hackathon aimed at evaluating the efforts undertaken by member states of the European Union to develop a European electronic health record exchange format. This format is intended to facilitate secure cross-border healthcare and optimize service delivery to citizens, paving the way toward a unified European health data space.

Methods

The hackathon was conducted within the scope of the X-eHealth project. Interested parties were initially presented with a representative clinical scenario and a set of specifications pertaining to the European electronic health record exchange format, encompassing Laboratory Results Reports, Medical Imaging and Reports, and Hospital Discharge Reports. In addition, five onboarding webinars and two professional training events were organized to support the participating entities. To ensure a minimum acceptable quality threshold, a set of inclusion criteria for participants was outlined for the interested teams.

Results

Eight teams participated in the hackathon, showcasing state-of-the-art applications. These teams utilized technologies such as Health Level Seven—Fast Healthcare Interoperability Resources (HL7 FHIR) and Clinical Document Architecture (CDA), alongside pertinent IHE integration profiles. They demonstrated a range of complementary uses and practices, contributing substantial inputs toward the development of future-proof electronic health record management systems.

Conclusions

The execution of the hackathon demonstrated the efficacy of such approaches in uniting teams from diverse backgrounds to develop state-of-the-art applications. The outcomes produced by the event serve as proof-of-concept demonstrators for managing and preventing chronic diseases, delivering value to citizens, companies, and the research community.
Regenerative endodontic procedures in immature permanent teeth with pulp necrosis: the impact of microbiology on clinical and radiographic outcome
Authors: Sellami Rayann , Van Holm Wannes , Meschi Nastaran , Van Den Heuvel Sarah , Pauwels Martine , Verspecht Tim , Vandamme Kathleen , Teughels Wim , Lambrechts Paul
Published: November 2023

Aims

The current study aimed to determine how the disinfection strategy for regenerative endodontic procedures (REPs) influences overall bacterial load and REP outcomes. Different bacterial species in the teeth were also examined in this study.

Methods

A previously reported non-randomized controlled clinical research on REP ± leukocyte and platelet-rich fibrin gathered microbial samples from 14 of 29 patients during REP (LPRF). Four microbiological samples were obtained in two treatment sessions. S1 and S2 were taken before and after the first irrigation with 1.5% NaOCl and saline. Samples S3 and S4 were obtained before and after rinsing with 17% EDTA in the second treatment session. Microbial samples were identified using a quantitative polymerase chain reaction with species-specific primers.

Results

The total bacterial load recovered from patients showed a significant (p < 0.05) decrease after the first treatment and was maintained throughout the second treatment. Fusobacterium nucleatum, Treponema denticola, and Enterococcus faecalis were the most prevalent species in root canals, detected in all analyzed cases (100%), followed by Prevotella intermedia and Tannerella forsythia, both in six of 14 (42.9%) cases. The presence of these abundant species was significantly reduced after sample S1 was obtained. Parvimonas micra was present in four of 14 (28.6%) cases and Actinomyces naeslundii in two of 14 (14.3%) cases. Filifactor alocis, Porphyromonas endodontalis, and Porphyromonas gingivalis were each detected in only one of 14 (7.1%) cases. No statistical correlation could be made between bacterial species and clinical or radiographic outcomes due to the small sample size. In the LPRF group, two cases required retreatment due to early post-treatment flare-up, and two other cases presented radiographically presented a persistent apical periodontitis 3 years after treatment. In the control group, all analyzed cases were clinically asymptomatic after treatment, and radiographically, the final periapical index score at the last recall revealed healthy periapices.

Conclusion

The REP disinfection protocol of the present study seems to be satisfactorily effective in reducing the total bacterial load, omitting clinical symptoms and inducing periapical bone healing in immature permanent teeth with pulp necrosis. However, LPRF seems to prevent these outcomes from being achieved and should consequently therefore not be recommended in REPs.
Case Report: Diabetes mellitus type MODY5 as a feature of 17q12 deletion syndrome with diabetic gastroparesis
Authors: Xin Sixu , Zhang Xiaomei
Published: November 2023

Background

Maturity-onset diabetes of the young type 5 (MODY5) is an uncommon, underrecognized condition that can be encountered in several clinical contexts. It is challenging to diagnose because it is considered rare and therefore overlooked in the differential diagnosis. Moreover, no typical clinical features or routine laboratory tests can immediately inform the diagnosis.

Case presentation

We report a 28-year-old man who was once misdiagnosed with type 1 diabetes due to decreased islet function and recurrent diabetic ketosis or ketoacidosis. However, he had intermittent nausea, vomiting, abdominal distension, and abdominal pain 6 months prior. Further examinations revealed agenesis of the dorsal pancreas, complex renal cyst, kidney stone, prostate cyst, hypomagnesaemia, and delayed gastric emptying. Accordingly, whole-exon gene detection was performed, and a heterozygous deletion mutation was identified at [GRCh37 (hg19)] chr17:34842526-36347106 (1.5 Mb, including HNF1B gene). The patient was eventually diagnosed with 17q12 deletion syndrome with gastroparesis.

Conclusion

We report a novel case of diabetes mellitus type MODY5 as a feature of 17q12 deletion syndrome caused by a new 17q12 deletion mutation, which will further broaden the genetic mutation spectrum of this condition. With the help of gene detection technology, these findings can assist endocrinologists in making the correct diagnosis of MODY5 or 17q12 deletion syndrome. Additionally, they can formulate an appropriate therapy and conduct genetic screening counseling for their family members to guide and optimize fertility.
Does endometrial compaction before embryo transfer affect pregnancy outcomes? a systematic review and meta-analysis
Authors: Chen Xiao-Tong , Sun Zhen-Gao , Song Jing-Yan
Published: November 2023

Objective

There is no clear evidence of clinical significance of endometrial compaction, which can be measured by a reduction in endometrial thickness (EMT) during the follicular-luteal transition before the day of embryo transfer. In this study, we aim to determine whether endometrial compaction has an effect on in vitro fertilization (IVF) success.

Method(s)

We searched PubMed, Cochrane, Embase, and Web of Science electronic databases for studies published in English up to March 2023. Heterogeneity between studies was assessed using the I2 statistic. The random effects model and fixed effects model was used to pool the risk ratio (RR) with a corresponding 95% confidence interval (CI). A subgroup analysis was performed based on different methods of ultrasonic measurement and different endometrial compaction rates (ECR). Stata 17.0 software was used for meta-analysis. Pregnancy outcomes, which included clinical pregnancy rate, ongoing pregnancy rate, live birth rate, and spontaneous abortion rate, were evaluated.

Result(s)

In this study, 18 cohort studies were included, involving 16,164 embryo transfer cycles. Pooled results indicated that there was no significant difference between the endometrial compaction group and the non-compaction group in terms of clinical pregnancy rate (RR [95% CI]=0.98 [0.90,1.08]; I2 = 69.76%), ongoing pregnancy rate (RR [95% CI]=1.18 [0.95,1.47]; I2 = 78.77%), live birth rate (RR [95% CI]= 0.97 [0.92,1.02]; I2 = 0.00%) or spontaneous abortion rate (RR [95% CI]= 1.07[0.97,1.26]; I2 = 0.00%). According to the subgroup analysis of ultrasonic measurement methods, in the transvaginal ultrasound (TVUS) combined with abdominal ultrasonography (AUS) cycles of the endometrial compaction group, the rate of ongoing pregnancy (RR [95% CI] = 1.69 [1.26, 2.26]; I2 = 29.27%) and live birth (RR [95% CI] = 1.27 [1.00,1.61]; I2 = 62.28%) was significantly higher than that of the non-compaction group. Additionally, subgroup analysis based on ECR revealed a significantly higher rate of ongoing pregnancy when ECR ≥ 15% (RR [95% CI] = 1.99 [1.61, 2.47]; I2 = 0.00%).

Conclusion

Endometrial compaction has no adverse effect on clinical pregnancy rate, ongoing pregnancy rate, live birth rate, or spontaneous abortion rate. A possible explanation for the contradictory findings of previous studies lies in the method by which the EMT is measured.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023430511, identifier CRD42023430511.
A novel approach for utilizing waste heat resources in the steel industry
Authors: Wang Ronggen , Zhang Wei , Yang Shaowei , Zhang Zhitao , Fan Suli , Lai Chun Sing
Published: November 2023

The efficient utilization of waste heat resources plays a pivotal role in enhancing energy efficiency and curbing carbon emissions. To address this, effective planning for waste heat recovery (WHR) utilization becomes imperative, guiding consumers in device installation and capacity allocation. This paper introduces a novel approach to WHR utilization planning, tailored specifically for steel factories, with the goal of achieving optimal WHR solutions. The approach automates device selection, capacity allocation, and operational strategies while considering their impact on the regular manufacturing processes of the factories to maximize overall benefits. Unlike existing methods, this approach introduces discrete capacity selection modeling, considering the constraints of the limited product range during device selection. A numerical study illustrates the effectiveness of the proposed model in delivering optimal WHR device selection, capacity allocation, and operational strategies under various economic conditions. These enhancements contribute to the increased practicality and realism of the proposed method in comparison to existing approaches.
Market power risk prevention mechanism of China’s electricity spot market based on stochastic evolutionary game dynamics
Authors: Xie Jingdong , Guan Bowen , Yao Yin , Li Ruizhen , Shi Quan
Published: November 2023

The construction of China’s power spot market is still in its early stage, with a high concentration on generation-side resources and frequent market power. It is urgent to establish risk prevention mechanisms for the generation of market power. First, the paper establishes a basic framework of the stochastic evolutionary game theory and then builds a “stochastic evolutionary game market-clearing” model for the market regulator and risk units. Second, the work provides a library of multi-dimensional monitoring and evaluation indicators for the regulator and creates a quantitative risk prevention strategy for the power spot market in China. Finally, an evolutionary dynamic analysis is conducted on players’ strategic evolution space and changes in market risks. Based on a simulation of actual data from an electricity market in China, it turns out that the generation-side market power risk prevention mechanism can lower market transaction and operational risks in a variety of power supply–demand scenarios. The study theoretically supports the development of market power risk prevention and provides more realistic insights into China’s power spot market as well.
Experimental evidence reveals that vector host preference and performance across host plants is not altered by vector-borne plant viruses
Authors: Clark Robert E. , Rincon Diego F. , Wu Ying , Crowder David W. , Eigenbrode Sanford D.
Published: November 2023

Many plant pathogens are transmitted by generalist insects that move between host species, and vector preference and performance on different hosts affects pathogen transmission. Most studies of host–vector–pathogen interactions focus on a single host, however, and it is relatively unknown if viruses affect the host breadth of vectors. For example, although pea aphids (Acyrthosiphon pisum) often form tight associations with specific hosts, viruses transmitted by aphids can break down host defenses, potentially weakening these associations and increasing the host breadth of their vectors. Here, we assessed vector host preference and performance in an experiment with five pea aphid colonies with distinct host associations, five host species, and two viruses (Pea enation mosaic virus [PEMV] and Bean leafroll virus [BLRV]). Our prediction was that pea aphids would have higher performance on their inferior hosts and be less discriminating among hosts when those are infected, compared with noninfected hosts. Our results show that aphid performance, measured as the rate of increase on hosts, differed among hosts based on their host association, and relative performance among hosts was indeed altered by virus infection. Aphid preferences, measured using a choice bioassay in which aphids could settle on any of the five host species, also differed among aphid colonies but was not affected by virus infection status. We also did not find that infection status of hosts altered the performance of aphids on their preferred host. Furthermore, virus infection did not disrupt preference performance relationships for the aphids. Overall, our study suggests that host preference and performance are deeply ingrained in pea aphids, and that host associations may be robust to the presence of host-manipulating plant pathogens.
Ligand-based targeting of c-kit using engineered γδ T cells as a strategy for treating acute myeloid leukemia
Authors: Branella Gianna M. , Lee Jasmine Y. , Okalova Jennifer , Parwani Kiran K. , Alexander Jordan S. , Arthuzo Raquel F. , Fedanov Andrew , Yu Bing , McCarty David , Brown Harrison C. , Chandrakasan Shanmuganathan , Petrich Brian G. , Doering Christopher B. , Spencer H. Trent
Published: November 2023

The application of immunotherapies such as chimeric antigen receptor (CAR) T therapy or bi-specific T cell engager (BiTE) therapy to manage myeloid malignancies has proven more challenging than for B-cell malignancies. This is attributed to a shortage of leukemia-specific cell-surface antigens that distinguish healthy from malignant myeloid populations, and the inability to manage myeloid depletion unlike B-cell aplasia. Therefore, the development of targeted therapeutics for myeloid malignancies, such as acute myeloid leukemia (AML), requires new approaches. Herein, we developed a ligand-based CAR and secreted bi-specific T cell engager (sBite) to target c-kit using its cognate ligand, stem cell factor (SCF). c-kit is highly expressed on AML blasts and correlates with resistance to chemotherapy and poor prognosis, making it an ideal candidate for which to develop targeted therapeutics. We utilize γδ T cells as a cytotoxic alternative to αβ T cells and a transient transfection system as both a safety precaution and switch to remove alloreactive modified cells that may hinder successful transplant. Additionally, the use of γδ T cells permits its use as an allogeneic, off-the-shelf therapeutic. To this end, we show mSCF CAR- and hSCF sBite-modified γδ T cells are proficient in killing c-kit+ AML cell lines and sca-1+ murine bone marrow cells in vitro. In vivo, hSCF sBite-modified γδ T cells moderately extend survival of NSG mice engrafted with disseminated AML, but therapeutic efficacy is limited by lack of γδ T-cell homing to murine bone marrow. Together, these data demonstrate preclinical efficacy and support further investigation of SCF-based γδ T-cell therapeutics for the treatment of myeloid malignancies.
Ecosystem-based Management through the lenses of International Seabed Authority stakeholders: current status, implications, and opportunities for the deep-sea mining regime in the Area
Authors: Guilhon Maila , Xavier Luciana Yokoyama , von Pogrell Luise , Singh Pradeep , Christiansen Sabine , Turra Alexander
Published: November 2023

The ocean is threatened by human activities, which undermine the health of its ecosystems. To overcome this scenario, there is a converging understanding that a more encompassing approach, such as Ecosystem-based Management (EBM), is essential to manage human activities. EBM implementation in scenarios of limited knowledge and potentially irreversible impacts, such as deep-sea mining (DSM), is thus highly appropriate, although the translation of this approach into practice is not intuitive and rather challenging. The International Seabed Authority (ISA), the organization with the mandate to award exploration and exploitation contracts for minerals on the international seabed, has recognized the need to incorporate EBM in its instruments but has not specified how to implement it. Through an online survey and in-depth interviews, ISA stakeholders have been inquired about their perception of the understanding, current status, implications, and opportunities of EBM for the deep-sea mining regime. The findings reveal that stakeholders perceive EBM as more related to ecological and impact aspects and less with participation, socio-ecological systems interlinks, and other forms of knowledge that are not scientific. Few respondents recognize EBM within the ISA, reporting its reflection in management instruments such as Regional Environmental Management Plans and Environmental Impact Assessments. No common definition exists, regarded as an obstacle to decision-making and EBM operationalization. According to them, opportunities to improve EBM implementation include collaboration with organizations already familiar with EBM, capacity development activities, workshops, and dedicated side events focusing on the issue. Finally, most of them recognize that a lack of consensus regarding EBM can impact decision-making and EBM operationalization, thus compromising ISA’s mandate. In this context, the clarification on what EBM entails for the seabed mining regime should be a matter of major interest to the ISA and all its stakeholders, as the mineral resources found in the seabed beyond the limits of national jurisdictions (the Area) are the common heritage of humankind, and therefore, its maintenance and benefits must be ensured to future generations.
Range wide genetic differentiation in the bull kelp Nereocystis luetkeana with a seascape genetic focus on the Salish Sea
Authors: Gierke Lily , Coelho Nelson C. , Khangaonkar Tarang , Mumford Tom , Alberto Filipe
Published: November 2023

Introduction

In temperate regions, one of the most critical determinants of present range-wide genetic diversity was the Pleistocene climate oscillations, the most recent one created by the last glacial maximum (LGM). This study aimed to describe N. luetkeana genetic structure across its entire range (Alaska to California) and test different models of population connectivity within the Salish Sea. This region was colonized after the LGM and has been under increased disturbance in recent decades.

Methods

We utilized microsatellite markers to study N. luetkeana genetic diversity at 53 sites across its range. Using higher sampling density in the Salish Sea, we employed a seascape genetics approach and tested isolation by hydrodynamic transport and environment models.

Results

At the species distribution scale, we found four main groups of genetic co-ancestry, Alaska; Washington with Vancouver Island’s outer coast and Juan de Fuca Strait; Washington’s inner Salish Sea; and Oregon with California. The highest allelic richness (AR) levels were found in California, near the trailing range edge, although AR was also high in Alaska. The inner Salish Sea region had the poorest diversity across the species distribution. Nevertheless, a pattern of isolation by hydrodynamic transport and environment was supported in this region.

Discussion

The levels of allelic, private allele richness and genetic differentiation suggest that during the LGM, bull kelp had both northern and southern glacial refugia in the Prince of Wales Island-Haida Gwaii region and Central California, respectively. Genetic diversity in Northern California sites seems resilient to recent disturbances, whereas the low levels of genetic diversity in the inner Salish Sea are concerning.
An elastic-viscoplastic creep model for describing creep behavior of layered rock
Authors: Li Yukun , Shen Mingxuan , Du Bin , Yuan Shisong
Published: November 2023

To describe the full-stage creep behavior of layered rock accurately, a new elastic-viscoplastic creep model is proposed based on fractional order theory in this manuscript, which consists of a Hooke elastomer, a fractional Abel dashpot, a Kelvin body, and a new non-linear visco-plastic component. The non-linear creep model can not only describe the changes in three creep stages (primary creep, steady-state creep and accelerating creep) but also reflect the influence of different bedding angles of rock. The constitutive equations of the non-linear creep model are deduced by the empirical model method and plastic theory method, respectively. The parameters of the non-linear creep model are identified using the Levenberg-Marquardt algorithm from Origin. It shows that the creep model in this paper are highly consistent with the experimental data under different load levels, creep stages and bedding angles, and the accuracy and rationality of the model are verified. Moreover, the creep constitutive equations for layered rock derived by the two methods have the same fitting effect on the same set of experimental data.
Vasculitis distribution and clinical characteristics in giant cell arteritis: a retrospective study using the new 2022 ACR/EULAR classification criteria
Authors: Andel Peter M. , Diamantopoulos Andreas P. , Myklebust Geirmund , Haugeberg Glenn
Published: November 2023

Introduction

Giant cell arteritis (GCA) is the most common vasculitis of the elderly. In recent years, advanced imaging has to a certain extent replaced temporal artery biopsy (TAB) to aid diagnosis in many institutions and helped to identify three major phenotypes of GCA, namely, cranial GCA (c-GCA), large-vessel non-cranial GCA (LV-GCA), and a combination of these two patterns called mixed-GCA, which all show different clinical patterns. Recent 2022 American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) classification criteria respect the changing conception and clinical practice during the last two decades. In this cohort study, we present vasculitis distribution and baseline characteristics using the 2022 ACR/EULAR classification criteria as well as the EULAR core data set.

Methods

In this retrospective study from Southern Norway, we identified all patients diagnosed with GCA between 2006 and 2019 in our single-center fast-track clinic (FTC). We included all patients who were examined using ultrasound (US) of cranial as well as non-cranial large vessels at diagnosis to depict vascular distribution. EULAR core data set, ACR 1990, and 2022 ACR/EULAR classification criteria were used to characterize the cohort.

Results

Seventy-seven patients were diagnosed with GCA at our institution in the aforementioned period. Seventy-one patients (92.2%) were diagnosed with the help of US and included in the further analysis. The 2022 ACR/EULAR classification criteria allocated 69 patients (97.2%), while the ACR 1990 classification criteria allocated 49 patients (69.0%) in our cohort as having GCA. Mixed-GCA was the most common type in 33 patients (46.5%). Weight loss was significantly more common in patients with large-vessel non-cranial vasculitis in LV-GCA and mixed-GCA. Headache, on the other hand, was significantly more common in patients with involvement of cranial vessels.

Conclusion

Mixed GCA was the most common form of GCA in our cohort. In our study, the 2022 ACR/EULAR classification criteria seem to be a more useful tool compared with the old ACR 1990 classification criteria to allocate GCA patients diagnosed and treated at our US-based FTC as having GCA.
Prokaryotic taxonomy and functional diversity assessment of different sequencing platform in a hyper-arid Gobi soil in Xinjiang Turpan Basin, China
Authors: Zhang Zhidong , Zhu Jing , Ghenijan Osman , Chen Jianwei , Wang Yuxian , Jiang Ling
Published: November 2023

Turpan Basin located in the eastern Xinjiang is a typical arid inland basin with extremely scarce water resources and a fragile ecosystem. Prokaryotic communities with unique genetic and physiological modifications can survive and function in such harsh environments, offering diverse microbial resources. However, numerous microbes can enter the viable but non-culturable state because of drought stress in the desert soil. In this work, next generation sequencing (NGS) technology based on DNA nanoball sequencing platform (DNBSEQ-G400) and sequencing-by-synthesis platform (NovaSeq 6000) were applied to analyze the prokaryotic diversity in three hyper-arid Gobi soils from Flaming Mountain, Toksun, and Kumtag. The comparison between two platforms indicated that DNBSEQ-G400 had better repeatability and could better reflect the prokaryotic community of this hyper-arid region. The diversity analysis based on DNBSEQ-G400 identified a total of 36 bacterial phyla, including Pseudomonadota, Bacteroidota, Bacillota, Actinomycetota, Methanobacteriota, Acidobacteriota, Nitrososphaerota, and Planctomycetota. The environmental factors, including soluble salt, available potassium, total nitrogen, and organic matter, were positively correlated with the abundance of most prokaryote. In addition, the prokaryotic community assembly in hyper-arid soil was well described by neutral-based models, indicating that the community assembly was mainly controlled by stochastic processes. Finally, the phylogenetic analysis of Actinomycetota proved that such extremophiles played an important role in the ecosystems they colonize. Overall, our result provides a reference for choosing the appropriate sequencing platform and a perspective for the utilization of soil microbial resources from hyper-arid regions.
Influence of different sample preparation techniques on imaging viruses and virus-like particles by scanning electron and scanning transmission electron microscopes
Authors: Kąkol Monika , Tagliasacchi Ezher , Borkowski Andrzej , Słowakiewicz Mirosław
Published: November 2023

Scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) were applied in many laboratories to visualize and image viruses and virus-like particles (VLPs). Two bacteriophages, P1 and Φ6, were chosen as model microorganisms known for their distinct structure, and viruses obtained from biofilms associated with modern travertines (Terme di Saturnia, Italy; Karahayıt “Kızılsu” and Pamukkale, Turkey) were also investigated. Three protocols, (1) full, (2) simplified, and (3) all at once were developed and tested for sample preparation and imaging. The full procedure enabled the observation of P1 bacteriophages, whereas the simplified protocol, successful in visualizing Φ6, did not yield satisfactory results for P1. The preservation state of the latter appeared to be compromised and led to less informative images in SEM and STEM. Viruses in biofilms exhibited various levels of mineralization and aggregation, complicating their characterization. In the all at once procedure, although effective in preserving bacteriophage tails, excessive coating and thickening of samples with heavy chemical reagents led to a reduction in overall image quality. Despite a final washing step, some residues of chemical reagents (OsO4 and uranyl acetate) remained, impacting the clarity of the images. Finally, the results obtained emphasize the critical role of sample preparation and imaging techniques in effectively visualizing and characterizing viruses and VLPs. The choice of analytical procedure significantly influences the resolution and preservation state of the observed bacteriophages and VLPs. It is suggested that the appropriate imaging technique is carefully selected based on the specific objectives of the project and the nature of the samples being investigated to obtain the best images of the viruses.
Dense captioning and multidimensional evaluations for indoor robotic scenes
Authors: Wang Hua , Wang Wenshuai , Li Wenhao , Liu Hong
Published: November 2023

The field of human-computer interaction is expanding, especially within the domain of intelligent technologies. Scene understanding, which entails the generation of advanced semantic descriptions from scene content, is crucial for effective interaction. Despite its importance, it remains a significant challenge. This study introduces RGBD2Cap, an innovative method that uses RGBD images for scene semantic description. We utilize a multimodal fusion module to integrate RGB and Depth information for extracting multi-level features. And the method also incorporates target detection and region proposal network and a top-down attention LSTM network to generate semantic descriptions. The experimental data are derived from the ScanRefer indoor scene dataset, with RGB and depth images rendered from ScanNet's 3D scene serving as the model's input. The method outperforms the DenseCap network in several metrics, including BLEU, CIDEr, and METEOR. Ablation studies have confirmed the essential role of the RGBD fusion module in the method's success. Furthermore, the practical applicability of our method was verified within the AI2-THOR embodied intelligence experimental environment, showcasing its reliability.
Role of circulating biomarkers in spinal muscular atrophy: insights from a new treatment era
Authors: Giorgia Querin , Gomez Garcia de la Banda Marta , Smeriglio Piera
Published: November 2023

Spinal muscular atrophy (SMA) is a lower motor neuron disease due to biallelic mutations in the SMN1 gene on chromosome 5. It is characterized by progressive muscle weakness of limbs, bulbar and respiratory muscles. The disease is usually classified in four different phenotypes (1–4) according to age at symptoms onset and maximal motor milestones achieved. Recently, three disease modifying treatments have received approval from the Food and Drug Administration (FDA) and the European Medicines Agency (EMA), while several other innovative drugs are under study. New therapies have been game changing, improving survival and life quality for SMA patients. However, they have also intensified the need for accurate biomarkers to monitor disease progression and treatment efficacy. While clinical and neurophysiological biomarkers are well established and helpful in describing disease progression, there is a great need to develop more robust and sensitive circulating biomarkers, such as proteins, nucleic acids, and other small molecules. Used alone or in combination with clinical biomarkers, they will play a critical role in enhancing patients’ stratification for clinical trials and access to approved treatments, as well as in tracking response to therapy, paving the way to the development of individualized therapeutic approaches. In this comprehensive review, we describe the foremost circulating biomarkers of current significance, analyzing existing literature on non-treated and treated patients with a special focus on neurofilaments and circulating miRNA, aiming to identify and examine their role in the follow-up of patients treated with innovative treatments, including gene therapy.
Encoding of continuous perceptual choices in human early visual cortex
Authors: Barbieri Riccardo , Töpfer Felix M. , Soch Joram , Bogler Carsten , Sprekeler Henning , Haynes John-Dylan
Published: November 2023

Introduction

Research on the neural mechanisms of perceptual decision-making has typically focused on simple categorical choices, say between two alternative motion directions. Studies on such discrete alternatives have often suggested that choices are encoded either in a motor-based or in an abstract, categorical format in regions beyond sensory cortex.

Methods

In this study, we used motion stimuli that could vary anywhere between 0° and 360° to assess how the brain encodes choices for features that span the full sensory continuum. We employed a combination of neuroimaging and encoding models based on Gaussian process regression to assess how either stimuli or choices were encoded in brain responses.

Results

We found that single-voxel tuning patterns could be used to reconstruct the trial-by-trial physical direction of motion as well as the participants’ continuous choices. Importantly, these continuous choice signals were primarily observed in early visual areas. The tuning properties in this region generalized between choice encoding and stimulus encoding, even for reports that reflected pure guessing.

Discussion

We found only little information related to the decision outcome in regions beyond visual cortex, such as parietal cortex, possibly because our task did not involve differential motor preparation. This could suggest that decisions for continuous stimuli take can place already in sensory brain regions, potentially using similar mechanisms to the sensory recruitment in visual working memory.
Poincaré plot can help predict the curative effect of metoprolol for pediatric postural orthostatic tachycardia syndrome
Authors: Yuan Piaoliu , Lian Zhouhui , Wang Yuanyuan , Zhang Chunyu , Jin Hongfang , Du Junbao , Huang Yaqian , Liao Ying
Published: November 2023

Purpose

To study whether a Poincaré plot can help predict the curative effect of metoprolol for postural orthostatic tachycardia syndrome (POTS) in children.

Methods

Pediatric patients with POTS who were administered metoprolol were retrospectively included. The collected data included general data (sex, age, height, weight, and body mass index), the manifestations and treatment (baseline orthostatic intolerance symptom score and course of metoprolol treatment), vital signs (supine heart rate [HR], supine blood pressure, and increased HR during the standing test), HR variability indexes (standard deviation of normal-to-normal intervals [SDNN]; standard deviation of the averages of normal-to-normal intervals [SDANN]; mean standard deviation of the NN intervals for each 5-min segment [SDNNI]; root mean square of the successive differences [rMSSD]; percentage of adjacent NN intervals that differ by >50 ms [pNN50]; triangular index; ultra-low [ULF], very low [VLF], low [LF], and high frequency [HF]; total power [TP]; and LF/HF ratio), and graphical parameters of the Poincaré plot (longitudinal axis [L], transverse axis [T], and L/T). Receiver operator characteristic curves were used to calculate the predictive function of the indexes with significant differences between patients who responded and those who did not. The index combination with the highest predictive value was obtained through series–parallel analysis.

Results

Overall, 40 responders and 23 non-responders were included. The L and T in the Poincaré plots and rMSSD, pNN50, HF, and TP of the HR variability data were significantly lower in participants who responded to metoprolol than in participants who did not (p < 0.001). The L/T of participants who responded to metoprolol was greater than that of non-responders (p < 0.001). Moreover, we noted a strong correlation between every two indexes among L, T, rMSSD, pNN50, HF, TP, and L/T (p < 0.05). T < 573.9 ms combined with L/T > 2.9 had the best performance for predicting the effectiveness of metoprolol, with a sensitivity of 85.0%, specificity of 82.6%, and accuracy of 84.1%.

Conclusion

In the Poincaré plot, a T < 573.9 ms combined with an L/T > 2.9 helps predict good outcomes of using metoprolol to treat pediatric POTS.
Assessment of color preference, purchase intention and sexual attractiveness of lipstick colors under multiple lighting conditions
Authors: Tian Baolin , Gong Hanwen , Chen Zhiyu , Yu Xuan , Pointer Michael R. , Yu Jie , Yu Feng , Liu Qiang
Published: November 2023

Lipstick is one of the most commonly used cosmetics, which is closely associated with female attractiveness and influences people’s perception and behavior. This study aimed to investigate the impact of light sources, lipstick colors, as well as gender on the subjective assessment of lipstick color products from the prospective of color preference, purchase intention and sexual attractiveness. The correlation between color preference evaluations when applying lipstick on lips and on forearms was also explored. Sixty participants completed their visual assessment of 15 lipsticks worn by 3 models under 5 light sources, with uniformly sampled correlated color temperature (CCT) values ranging from 2,500 K to 6,500 K. The results indicated that the light source significantly influenced color preference and purchase intention, while lipstick color significantly impacted on sexual attractiveness. The interactions between gender and other factors were also observed and are discussed. Compared to men, women were found to be more sensitive to different light sources and hold different attitudes toward different lipstick colors under different CCTs. Interestingly, no significant correlation was found between lipstick color preference ratings on the lips and forearm, which conflicted with the commonly recognized way of lipstick color selection. These findings should contribute to a deeper understanding of the consumer attitude toward lipstick colors and provide a useful reference for lighting design in situations where cosmetics are specified, manufactured, retailed and generally used, both professionally and in the home.
Mice deficient in complement C3 are protected against recognition memory deficits and astrogliosis induced by status epilepticus
Authors: Schartz Nicole D. , Aroor Alisha , Li Yibo , Pinzón-Hoyos Nicole , Brewster Amy L.
Published: November 2023

Introduction

Status epilepticus (SE) can significantly increase the risk of temporal lobe epilepsy (TLE) and cognitive comorbidities. A potential candidate mechanism underlying memory defects in epilepsy may be the immune complement system. The complement cascade, part of the innate immune system, modulates inflammatory and phagocytosis signaling, and has been shown to contribute to learning and memory dysfunctions in neurodegenerative disorders. We previously reported that complement C3 is elevated in brain biopsies from human drug-resistant epilepsy and in experimental rodent models. We also found that SE-induced increases in hippocampal C3 levels paralleled the development of hippocampal-dependent spatial learning and memory deficits in rats. Thus, we hypothesized that SE-induced C3 activation contributes to this pathophysiology in a mouse model of SE and acquired TLE.

Methods

In this study C3 knockout (KO) and wild type (WT) mice were subjected to one hour of pilocarpine-induced SE or sham conditions (control; C). Following a latent period of two weeks, recognition memory was assessed utilizing the novel object recognition (NOR) test. Western blotting was utilized to determine the protein levels of C3 in hippocampal lysates. In addition, we assessed the protein levels and distribution of the astrocyte marker glial fibrillary acidic protein (GFAP).

Results

In the NOR test, control WT + C or C3 KO + C mice spent significantly more time exploring the novel object compared to the familiar object. In contrast, WT+SE mice did not show preference for either object, indicating a memory defect. This deficit was prevented in C3 KO + SE mice, which performed similarly to controls. In addition, we found that SE triggered significant increases in the protein levels of GFAP in hippocampi of WT mice but not in C3 KO mice.

Discussion

These findings suggest that ablation of C3 prevents SE-induced recognition memory deficits and that a C3-astrocyte interplay may play a role. Therefore, it is possible that enhanced C3 signaling contributes to SE-associated cognitive decline during epileptogenesis and may serve as a potential therapeutic target for treating cognitive comorbidities in acquired TLE.
Immunomodulatory effect of marine lipids on food allergy
Authors: Abril Ana G. , Carrera Mónica , Pazos Manuel
Published: November 2023

Seafood is highly enriched in n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFAs), particularly eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3), in contrast to the ultra-processed foods included in the modern Western diet that have high levels of n-6 linoleic acid (LA, 18:2 n-6), precursor for the pro-inflammatory n-6 arachidonic acid (ARA, 20:4 n-6). The capacity of marine lipids to reduce plasmatic triglycerides and blood pressure have been well-described. Moreover, recent studies have also raised evidence of a potential regulatory action of marine lipids on inflammation, the immune system, and food allergy (FA). FA is considered one of the main concerns to become life threatening in food safety. The prevalence of this emerging global problem has been increasing during the last two decades, especially in industrialized countries. About a 6-8% of young children and 2-4% of adults is estimated to be affected by FA. The main objective of the current study is to update the existing knowledge, but also the limitations, on the potential impact of marine lipids and their lipid mediators in regulating immunity, inflammation, and ultimately, food allergies. In particular, the focus is on the effect of marine lipids in modulating the key factors that control the sensitization and effector phases of FA, including gut microbiota (GM), inflammation, and immune system response. Results in animal models highlight the positive effect that consuming marine lipids, whether as a supplement or through seafood consumption, may have a relevant role in improving gut dysbiosis and inflammation, and preventing or reducing the severity of FA. However, more systematic studies in humans are needed to optimize such beneficial actions to each particular FA, age, and medical condition to reach an effective clinical application of marine lipids to improve FAs and their outcomes.