bluuebunny/crossref_metadata_2025 huggingface dataset:
Improved Performance of All-Solid-State Flexible Supercapacitor Based on the Stress-Compensation Effect
Authors: Wang D. Y. , Dong Z. Q. , Zhang S. , Hu T. Y. , Zhang X. T. , Li X. , Li F.
Published: January 2021

This work presents a facile strategy to develop a flexible polyaniline (PANI)-based supercapacitor (SC) with both high energy density and good capacitance retention. An electrode with a symmetrical sandwich-structured configuration (PANI/flexible porous support/PANI) is used as both
working and counter electrodes for this supercapacitor. For a conventional electrode with PANI depositing on single side of the support (PANI/flexible support), the flexible support bends severely during the PANI electrodeposition process, which results in poor PANI deposition. On the contrary,
for the symmetrical sandwich-structured electrode, due to the stress-compensation effect induced by this configuration, the support bending is significantly suppressed and thus PANI films with a good uniformity are realized. Moreover, the stress-compensation effect involved in the symmetrical
sandwichstructured electrode can also effectively balance the stress caused by PANI expansion/shrinkage during its electrochemical charge/discharge operation, thus improving the mechanical stability. The symmetrical sandwich-structured electrode has larger PANI mass loading, better PANI morphologies
and stronger mechanical stability than those of the conventional electrode. Consequently, the SC constructed by the symmetrical sandwich-structured electrode displays better electrochemical performance in terms of its larger specific areal capacitance (369.2 mF·cm−2
at a current density of 0.25 mA·cm−2), higher energy density (0.031 mWh·cm−2 at a power density of 1.21 mW·cm−2) and better cycling retention (93.2% of the retained capacity over 6000 cycles) than the SC constructed by
the conventional electrode.
MRSA Causing Necrotizing Fasciitis in 18-Month-Old Boy
Authors: Jain Avani , Meher Ravi , Sharma Raman
Published: January 2021

Necrotizing fasciitis is a rare and fulminant infection of the superficial fascia and subcutaneous cellular tissue. It is commonly polymicrobial, with the combination of aerobic and anaerobic bacteria, which contributes to the rapid progression and severity of the disease. The microbes commonly involved include group A streptococcus, Enterobacteriaceae, anaerobes, and Staphylococcus aureus. Over the past few years, skin and soft tissue infections, including necrotizing fasciitis, due to methicillin-resistant Staphylococcus aureus are increasing.
The Interplay Between Mathematical and Computational Thinking in Primary School Students’ Mathematical Problem-Solving Within a Programming Environment
Authors: Cui Zhihao , Ng Oi-Lam
Published: January 2021

In this paper, we explore the challenges experienced by a group of Primary 5 to 6 (age 12–14) students as they engaged in a series of problem-solving tasks through block-based programming. The challenges were analysed according to a taxonomy focusing on the presence of computational thinking (CT) elements in mathematics contexts: preparing problems, programming, create computational abstractions, as well as troubleshooting and debugging. Our results suggested that the challenges experienced by students were compounded by both having to learn the CT-based environment as well as to apply mathematical concepts and problem solving in that environment. Possible explanations for the observed challenges stemming from differences between CT and mathematical thinking are discussed in detail, along with suggestions towards improving the effectiveness of integrating CT into mathematics learning. This study provides evidence-based directions towards enriching mathematics education with computation.
Experimental conditions influence the formation and composition of the corona around gold nanoparticles
Authors: Hossen Md. Nazir , Elechalawar Chandra Kumar , Sjoelund Virginie , Moore Kathleen , Mannel Robert , Bhattacharya Resham , Mukherjee Priyabrata
Published: January 2021

Background

Ovarian cancer is one of the deadliest gynecological malignancies. While the overall survival of ovarian cancer patients has slightly improved in recent years in the developed world, it remains clinically challenging due to its frequent late diagnosis and the lack of reliable diagnostic and/or prognostic markers. The aim of this study was to identify potential new molecular target proteins (NMTPs) responsible for the poor outcomes. When nanoparticles (NP) are exposed to biological fluids, a protein coat, termed the protein corona (PC), forms around the NP, and the PC represents a tool to identify NMTPs. This study investigates the influence of pre-processing conditions, such as lysis conditions and serum/plasma treatment, on the PC composition and the resulting identification of NMTPs.

Results

Using gel electrophoresis, pre-processing conditions, including cell-lysis techniques and enrichment of low-abundance proteins (LAPs) by immunocentrifugation of serum/plasma, were shown to alter the relative amounts and compositions of proteins. PCs formed when 20 nm gold-NPs (GNPs) were incubated with lysate proteins from either RIPA- or urea lysis. Proteomic analysis of these PCs showed 2–22-fold enrichment of NMTPs in PCs from urea lysates as compared to RIPA lysates. Enriched NMTPs were then classified as cellular components, biological and molecular functions-associated proteins. The impact of enriched LAPs (eLAPs) on both PC composition and NMTP identification was shown by comparative proteomic analysis of original plasma, eLAPs, and PCs derived from eLAPs; eLAPs-PCs enhanced the abundance of NMTPs approximately 13%. Several NMTPs, including gasdermin-B, dermcidin, and kallistatin, were identified by this method demonstrating the potential use of this PC approach for molecular target discovery.

Conclusion

The current study showed that the pre-processing conditions modulate PC composition and can be used to enhance identification of NMTPs.
What a weight loss programme should contain if people with obesity were asked - a qualitative analysis within the DO:IT study
Authors: Jessen-Winge Christina , Ilvig Pia Maria , Fritz Heather , Brandt Carl J. , Lee Kim , Christensen Jeanette Reffstrup
Published: January 2021

Background

Currently 1.9 billion adults worldwide are estimated to be overweight or obese. In Denmark the municipalities hold the responsibility to deliver weight loss programmes to overweight and obese citizens. There is a tendency to assume that weight loss programmes that show positive effects in specialized hospital settings are directly transferrable to municipal settings. However, municipality-based weight loss programmes have not produced clinically significant reductions in body weight. One reason for this may be that much research evidence regarding obesity programming neglects the perspectives of people with obesity. The first step in developing a weight loss programme designed for municipal settings is to understand what people with obesity want and need from a programme. The aim of this study was to examine what people with obesity find important in a weight loss programme for weight loss and weight maintenance.

Methods

We used a qualitative, explorative, descriptive design with individual interviews. We included men and women age 17 and older with a BMI ≥ 25 kg/m2. Participants were recruited from the wait lists of 13 municipality programmes and through Facebook posts. Data were analyzed using content analysis.

Results

Thirty-four participants with overweight or obesity were individually interviewed (ages between 19 and 74). Findings suggest that weight loss programmes should; a) support participants in structuring days; b) consider the use of replacement activities to reduce cognitive and emotional burden; c) aide individuals to increase self-efficacy and; d) include family and friends as well as health professionals and peers in the weight loss process. Diet and exercise, while important, should be balanced with other meaningful activities in everyday life.

Conclusion

Participants in this study wished to balance weight loss related activities with overall everyday life as well as finding the believe in their ability to lose weight in social relations.
Efficacy and safety of Si-Jun-Zi-Tang-based therapies for functional (non-ulcer) dyspepsia: a meta-analysis of randomized controlled trials
Authors: Wang Yaping , Liu Bin , Fu Xiuqiong , Tong Tiejun , Yu Zhiling
Published: January 2021

Background

The traditional Chinese medicine formula Si-Jun-Zi-Tang (SJZT) has a long history of application in the treatment of functional dyspepsia (non-ulcer dyspepsia, FD)-like symptoms. SJZT-based therapies have been claimed to be beneficial in managing FD. This study aimed to assess the efficacy and safety of SJZT-based therapies in treating FD by meta-analysis.

Methods

Systematic searches for RCTs were conducted in seven databases (up to February 2019) without language restrictions. Data were analyzed using Cochrane RevMan software version 5.3.0 and Stata software version 13.1, and reported as relative risk (RR) or odds ratio (OR) with 95% confidence intervals (CIs). The primary outcome was response rate and the secondary outcomes were gastric emptying, quality of life, adverse effects and relapse rate. The quality of evidence was evaluated according to criteria from the Cochrane risk of bias.

Results

A total of 341 potentially relevant publications were identified, and 12 RCTs were eligible for inclusion. For the response rate, there was a statically significant benefit in favor of SJZT-based therapies (RR = 1.23; 95% CI 1.17 to 1.30). However, the benefit was limited to modified SJZT (MSJZT). The relapse rate of FD patients received SJZT-based therapies was lower than that of patients who received conventional medicines (OR = 0.23; 95% CI 0.10 to 0.51). No SJZT-based therapies-related adverse effect was reported.

Conclusion

SJZT-based prescriptions may be effective in treating FD and no serious side-effects were identified, but the effect on response rate appeared to be limited to MSJZT. The results should be interpreted with caution as all the included studies were considered at a high risk of bias. Standardized, large-scale and strictly designed RCTs are needed to further validate the benefits of SJZT-based therapies for FD management.

Trial registration

Systematic review registration: [PROSPERO registration: CRD42019139136].
A standardized polyherbal preparation POL-6 diminishes alcohol withdrawal anxiety by regulating Gabra1, Gabra2, Gabra3, Gabra4, Gabra5 gene expression of GABAA receptor signaling pathway in rats
Authors: Sharma Lalit , Sharma Aditi , Dash Ashutosh Kumar , Bisht Gopal Singh , Gupta Girdhari Lal
Published: January 2021

Background

Alcohol abuse is a major problem worldwide and it affects people’s health and economy. There is a relapse in alcohol intake due to alcohol withdrawal. Alcohol withdrawal anxiety-like behavior is a symptom that appears 6–24 h after the last alcohol ingestion.

Methods

The present study was designed to explore the protective effect of a standardized polyherbal preparation POL-6 in ethanol withdrawal anxiety in Wistar rats. POL-6 was prepared by mixing the dried extracts of six plants Bacopa monnieri, Hypericum perforatum, Centella asiatica, Withania somnifera, Camellia sinesis, and Ocimum sanctum in the proportion 2:1:2:2:1:2 respectively. POL-6 was subjected to phytochemical profiling through LC-MS, HPLC, and HPTLC. The effect of POL-6 on alcohol withdrawal anxiety was tested using a two-bottle choice drinking paradigm model giving animals’ free choice between alcohol and water for 15 days. Alcohol was withdrawn on the 16th day and POL-6 (20, 50, and 100 mg/kg, oral), diazepam (2 mg/kg) treatment was given on the withdrawal days. Behavioral parameters were tested using EPM and LDT. On the 18th day blood was collected from the retro-orbital sinus of the rats and alcohol markers ALT, AST, ALP, and GGT were studied. At end of the study, animals were sacrificed and the brain was isolated for exploring the influences of POL-6 on the mRNA expression of GABAA receptor subunits in the amygdala and hippocampus.

Results

Phytochemical profiling showed that POL-6 contains major phytoconstituents like withaferin A, quercetin, catechin, rutin, caeffic acid, and β-sitosterol. In-vivo studies showed that POL-6 possesses an antianxiety effect in alcohol withdrawal. Gene expression studies on the isolated brain tissues showed that POL-6 normalizes the GABAergic transmission in the amygdala and hippocampus of the rats.

Conclusion

The study concludes that POL-6 may have therapeutic potential for treating ethanol-type dependence.
Patterns of flea infestation in rodents and insectivores from intensified agro-ecosystems, Northwest Spain
Authors: Herrero-Cófreces Silvia , Flechoso Manuel Fabio , Rodríguez-Pastor Ruth , Luque-Larena Juan José , Mougeot François
Published: January 2021

Background

Fleas frequently infest small mammals and play important vectoring roles in the epidemiology of (re)emerging zoonotic disease. Rodent outbreaks in intensified agro-ecosystems of North-West Spain have been recently linked to periodic zoonotic diseases spillover to local human populations. Obtaining qualitative and quantitative information about the composition and structure of the whole flea and small mammal host coexisting communities is paramount to understand disease transmission cycles and to elucidate the disease-vectoring role of flea species. The aims of this research were to: (i) characterise and quantify the flea community parasiting a small mammal guild in intensive farmlands in North-West Spain; (ii) determine and evaluate patterns of co-infection and the variables that may influence parasitological parameters.

Methods

We conducted a large-scale survey stratified by season and habitat of fleas parasitizing the small mammal host guild. We report on the prevalence, mean intensity, and mean abundance of flea species parasitizingMicrotus arvalis,Apodemus sylvaticus,Mus spretusandCrocidura russula. We also report on aggregation patterns (variance-to-mean ratio and discrepancy index) and co-infection of hosts by different flea species (Fager index) and used generalized linear mixed models to study flea parameter variation according to season, habitat and host sex.

Results

Three flea species dominated the system:Ctenophthalmus apertus gilcolladoi, Leptopsylla taschenbergiandNosopsyllus fasciatus. Results showed a high aggregation pattern of fleas in all hosts. All host species in the guild sharedC. a. gilcolladoiandN. fasciatus, butL. taschenbergimainly parasitized mice (M. spretusandA. sylvaticus). We found significant male-biased infestation patterns in mice, seasonal variations in flea abundances for all rodent hosts (M. arvalis, M. spretusandA. sylvaticus), and relatively lower infestation values for voles inhabiting alfalfas. Simultaneous co-infections occurred in a third of all hosts, andN. fasciatuswas the most common flea co-infecting small mammal hosts.

Conclusions

The generalistN. fasciatusandC. a. gilcolladoidominated the flea community, and a high percentage of co-infections with both species occurred within the small mammal guild.Nosopsyllus fasciatusmay show higher competence of inter-specific transmission, and future research should unravel its role in the circulation of rodent-borne zoonoses.

Graphical Abstract
Micro-RNA-125a mediates the effects of hypomethylating agents in chronic myelomonocytic leukemia
Authors: Berg Johannes Lorenz , Perfler Bianca , Hatzl Stefan , Mayer Marie-Christina , Wurm Sonja , Uhl Barbara , Reinisch Andreas , Klymiuk Ingeborg , Tierling Sascha , Pregartner Gudrun , Bachmaier Gerhard , Berghold Andrea , Geissler Klaus , Pichler Martin , Hoefler Gerald , Strobl Herbert , Wölfler Albert , Sill Heinz , Zebisch Armin
Published: January 2021

Background

Chronic myelomonocytic leukemia (CMML) is an aggressive hematopoietic malignancy that arises from hematopoietic stem and progenitor cells (HSPCs). Patients with CMML are frequently treated with epigenetic therapeutic approaches, in particular the hypomethylating agents (HMAs), azacitidine (Aza) and decitabine (Dec). Although HMAs are believed to mediate their efficacy via re-expression of hypermethylated tumor suppressors, knowledge about relevant HMA targets is scarce. As silencing of tumor-suppressive micro-RNAs (miRs) by promoter hypermethylation is a crucial step in malignant transformation, we asked for a role of miRs in HMA efficacy in CMML.

Results

Initially, we performed genome-wide miR-expression profiling in a KrasG12D-induced CMML mouse model. Selected candidates with prominently decreased expression were validated by qPCR in CMML mice and human CMML patients. These experiments revealed the consistent decrease in miR-125a, a miR with previously described tumor-suppressive function in myeloid neoplasias. Furthermore, we show that miR-125a downregulation is caused by hypermethylation of its upstream region and can be reversed by HMA treatment. By employing both lentiviral and CRISPR/Cas9-based miR-125a modification, we demonstrate that HMA-induced miR-125a upregulation indeed contributes to mediating the anti-leukemic effects of these drugs. These data were validated in a clinical context, as miR-125a expression increased after HMA treatment in CMML patients, a phenomenon that was particularly pronounced in cases showing clinical response to these drugs.

Conclusions

Taken together, we report decreased expression of miR-125a in CMML and delineate its relevance as mediator of HMA efficacy within this neoplasia.
RETRACTED ARTICLE: Mesenchymal stem cell-derived exosome miR-542-3p suppresses inflammation and prevents cerebral infarction
Authors: Cai Guofeng , Cai Guoliang , Zhou Haichun , Zhuang Zhe , Liu Kai , Pei Siying , Wang Yanan , Wang Hong , Wang Xin , Xu Shengnan , Cui Cheng , Sun Manchao , Guo Sihui , Jia Kunping , Wang Xiuzhen , Zhang Dianquan
Published: January 2021

Background

Cerebral infarction ranks as the second leading cause of disability and death globally, and inflammatory response of glial cells is the main cause of brain damage during cerebral infarction.

Methods

Studies have shown that mesenchymal stem cells (MSCs) can secrete exosomes and contribute to cerebral disease. Here, we would explore the function of MSC-derived exosome in cerebral infarction.

Results

Microarray indicated a decrease of miR-542-3p and an increase of Toll-Like Receptor 4 (TLR4) in middle cerebral artery occlusion (MCAO) mice comparing with sham mice. And luciferase and RIP analysis indicated a binding of miR-542-3p and TLR4. Then, we injected AAV9-miR-542-3p into paracele of sham or MCAO mice. Functional analysis showed that AAV9-miR-542-3p inhibited infarction area and the number of degenerating neurons and suppressed inflammatory factors’ expression and inflammatory cell infiltration. As well, transfection of miR-542-3p mimics into HA1800 cells underwent oxygen and glucose deprivation (OGD). Similarly, overexpression of miR-542-3p alleviated OGD induced cell apoptosis, ROS, and activation of inflammation response. Moreover, miR-542-3p could be packaged into MSCs and secreted into HA1800 cells. The extractive exosome-miR-21-3p treatment relieved MCAO- or OGD-induced cerebral injury and inflammation through targeting TLR4.

Conclusion

These results confirmed that MSC-derived exosome miR-542-3p prevented ischemia-induced glial cell inflammatory response via inhibiting TLR4. These results suggest possible therapeutic strategies for using exosome delivery of miR-542-3p to cure cerebral ischemic injury.
Investigation of interleukin-2-mediated changes in blood pressure, fetal growth restriction, and innate immune activation in normal pregnant rats and in a preclinical rat model of preeclampsia
Authors: Cunningham Mark W. , Amaral Lorena M. , Campbell Nathan E. , Cornelius Denise C. , Ibrahim Tarek , Vaka Venkata Ramana , LaMarca Babbette
Published: January 2021

Two important clinical features of preeclampsia (PE) are hypertension and fetal growth restriction. The reduced uterine perfusion pressure (RUPP) preclinical rat model of PE exhibits both of these features. Moreover, RUPP and PE women have elevated vasoconstrictor peptide endothelin-1 (ET-1) and inflammation. Interleukin-2 (IL-2) is a cytokine that regulates NK cell activity and is elevated in miscarriage, PE, and RUPP rats. The objective of this study was to examine a role for IL-2 in NK cell activation, fetal growth restriction, and hypertension during pregnancy by either infusion of IL-2 or blockade of IL-2 (basiliximab) in normal pregnant (NP) and RUPP rats. On gestational day 14, NP and RUPP rats received low (LD), middle (MD), or high dose (HD) IL-2 (0.05, 0.10, or 0.20 ng/ml) IP or basiliximab (0.07 mg per rat) by IV infusion. On day 19, blood pressure (MAP), pup weights, and blood were collected. Basiliximab had no effect on blood pressure, however, significantly lowered NK cells and may have worsened overall fetal survival in RUPP rats. However, IL-2 LD (102 ± 4 mmHg) and IL-2 HD (105 ± 6 mmHg) significantly lowered blood pressure, ET-1, and activated NK cells compared to control RUPPs (124 ± 3 mmHg,p< 0.05). Importantly, IL-2 in RUPP rats significantly reduced fetal weight and survival. These data indicate that although maternal benefits may have occurred with low dose IL-2 infusion, negative effects were seen in the fetus. Moreover, inhibition of IL-2 signaling did not have favorable outcome for the mother or fetus.
Enzyme additives influence bacterial communities of Medicago sativa silage as determined by Illumina sequencing
Authors: Hu Zongfu , Ma Deying , Niu Huaxin , Chang Jie , Yu Jianhua , Tong Qing , Li Shuguo
Published: January 2021

The goal of the present study was to evaluate the effects of enzymes (cellulase combined with galactosidase) and their combination with Lactobacillus plantarum (LP) on bacterial diversity in alfalfa silages using high-throughput sequencing. Alfalfa forages were treated with or without cellulase + ɑ-galactosidase (CEGA), cellulase + LP (CELP), or ɑ-galactosidase + LP (GALP). After 56 days of ensiling, all treated silages exhibited improved fermentation quality, as reflected by decreased pH, ammonium-N and increased lactic acid levels compared to the control silage (P < 0.05). Enzymatic treatment improved nutrient value by increasing crude protein levels and decreasing neutral detergent fibre (NDF) levels (P < 0.05). Silage treatment significantly altered the bacterial community, as determined by PCoA (P < 0.05). Lactic acid bacteria (LAB) dominated the bacterial community of the treated silage after ensiling. The dominant bacteria changed from Garciella, Enterococcus, Lactobacillus and Pediococcus in the control silage to Lactobacillus and Pediococcus in the CEGA silage and Lactobacillus in the CELP and GALP silages. Collectively, these results suggest that treatment with both enzymes alone and in combination with inoculants greatly increased the abundance of LAB, with Enterococcus, Lactobacillus and Pediococcus observed in the silage treated with enzymes alone (CEGA) and Lactobacillus observed in the silage treated with a combination of enzymes and inoculants (CELP and GALP).
Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum
Authors: Roberts Daniel P. , Selmer Kaitlyn , Lupitskyy Robert , Rice Clifford , Buyer Jeffrey S. , Maul Jude E. , Lakshman Dilip K. , DeSouza Jorge
Published: January 2021

Ethanol extract of cell mass of Serratia marcescens strain N4-5, when applied as a treatment to cucumber seed, has been shown to provide control of the oomycete soil-borne plant pathogen Pythium ultimum equivalent to that provided by a seed-treatment chemical pesticide in some soils. Two dominant compounds in this extract, prodigiosin and the serratamolide serrawetin W1, were identified based on mass and collision induced dissociation mass fragmentation spectra. An additional four compounds with M+H+ masses (487, 541, 543, and 571) consistent with serratamolides reported in the literature were also detected. Several other compounds with M+H+ masses of 488, 536, 684, 834, 906, and 908 m/z were detected in this ethanol extract inconsistently over multiple liquid chromatography coupled with tandem mass spectrometry (LC/MS–MS) runs. A purified preparation of prodigiosin provided control of damping-off of cucumber caused by P. ultimum when applied as a seed treatment while ethanol extract of cell mass of strain Tn246, a transposon-mutant-derivative of strain N4-5, did not. Strain Tn246 contained a mini-Tn5 Km insertion in a prodigiosin biosynthetic gene and was deficient in production of prodigiosin. All other compounds detected in N4-5 extract were detected in the Tn246 extract. This is the first report demonstrating that prodigiosin can control a plant disease. Other compounds in ethanol extract of strain N4-5 may contribute to disease control.
Interleukin-22 and connective tissue diseases: emerging role in pathogenesis and therapy
Authors: Xuan Xiuyun , Zhang Lin , Tian Chunxia , Wu Ting , Ye Haihua , Cao Juanmei , Chen Fangqi , Liang Yan , Yang Huilan , Huang Changzheng
Published: January 2021

Interleukin-22 (IL-22), a member of the IL-10 family of cytokines, is produced by a number of immune cells involved in the immune microenvironment of the body. IL-22 plays its pivotal roles by binding to the IL-22 receptor complex (IL-22R) and subsequently activating the IL-22R downstream signalling pathway. It has recently been reported that IL-22 also contributes to the pathogenesis of many connective tissue diseases (CTDs). In this review, we will discuss the role of IL-22 in several CTDs, such as system lupus erythematosus, rheumatoid arthritis, Sjögren’s syndrome, systemic sclerosis and dermatomyositis, suggesting that IL-22 may be a potential therapeutic target in CTDs.
Identification of predictors based on drug targets highlights accurate treatment of goserelin in breast and prostate cancer
Authors: Zhao Yue , Sun Huimin , Zheng Jianzhong , Shao Chen , Zhang Dongwei
Published: January 2021

Goserelin is an effective alternative to surgery or estrogen therapy in prostate cancer palliation, and possibly to ovariectomy in premenopausal breast cancer. However, not all users of goserelin can benefit from it, or some patients are not sensitive to goserelin. The advent of network pharmacology has highlighted the need for accurate treatment and predictive biomarkers. In this study, we successfully to identify 76 potential targets related to the compound of goserelin through network pharmacology approach. We also identified 18 DEGs in breast cancer tissues and 5 DEGs in cells, and 6 DEGs in prostate cancer tissues and 9 DEGs in cells. CRABP2 is the common DEG both in breast and prostate cancer. The risk prediction models constructed with potential prognostic targets of goserelin can successfully predict the prognosis in breast and prostate cancer, especially for very young breast cancer patients. Moreover, seven subgroups in breast cancer and six subgroups in prostate cancer were respectively identified based on consensus clustering using potential prognostic targets of goserelin that significantly influenced survival. The expression of representative genes including CORO1A and ANXA5 in breast and DPP4 in prostate showed strong correlations with clinic-pathological factors. Taken together, the novel signature can facilitate identification of new biomarkers which sensitive to goserelin, increase the using accuracy of goserelin and clarify the classification of disease molecular subtypes in breast and prostate cancer.
An efficient adaptive grid method for a system of singularly perturbed convection-diffusion problems with Robin boundary conditions
Authors: Liu Li-Bin , Liang Ying , Bao Xiaobing , Fang Honglin
Published: January 2021

A system of singularly perturbed convection-diffusion equations with Robin boundary conditions is considered on the interval$[0,1]$[0,1]. It is shown that any solution of such a problem can be expressed to a system of first-order singularly perturbed initial value problem, which is discretized by the backward Euler formula on an arbitrary nonuniform mesh. An a posteriori error estimation in maximum norm is derived to design an adaptive grid generation algorithm. Besides, in order to establish the initial values of the original problems, we construct a nonlinear optimization problem, which is solved by the Nelder–Mead simplex method. Numerical results are given to demonstrate the performance of the presented method.
Systematic review of situational prevention methods for crime against species
Authors: Delpech Dorothea , Borrion Herve , Johnson Shane
Published: January 2021

Illegal activities concerning terrestrial species (TS) are responsible for a variety of health, environmental, economic and security issues. The majority of academic research associated with species relates to conservation, with few publications specifically investigating the scale of crimes impacting species or how they can be prevented. This article systematically reviews the available evidence about what works to prevent crime against terrestrial species. Of over 29,000 documents that were returned in the first stage of the review, these were filtered to just over 100. The remaining documents were partially or fully read to identify the most relevant documents to include in the final qualitative synthesis. The review results show there is a significant lack of primary research in this area, as only five articles were found that met the study inclusion criteria. The identified articles focus on the effects of two types of situational crime prevention interventions: community outreach and ranger patrol frequency. Community outreach was shown to have a significant impact on local poaching levels, while for patrolling the evidence suggests a positive impact on the discovery of poachers, animal carcasses and poaching paraphernalia, however, the quality of these studies varied greatly. To prevent the further decline of species numbers internationally, more effort should be invested in publicising existing research into the effectiveness of prevention strategies that have not reached the wider scientific audience, as well as the funding and promotion of research into alternate methods of crime prevention.
Microbial diversity of co-occurring heterotrophs in cultures of marine picocyanobacteria
Authors: Kearney Sean M. , Thomas Elaina , Coe Allison , Chisholm Sallie W.
Published: January 2021

Background

The cyanobacteria Prochlorococcus and Synechococcus are responsible for around 10% of global net primary productivity, serving as part of the foundation of marine food webs. Heterotrophic bacteria are often co-isolated with these picocyanobacteria in seawater enrichment cultures that contain no added organic carbon; heterotrophs grow on organic carbon supplied by the photolithoautotrophs. For examining the selective pressures shaping autotroph/heterotroph interactions, we have made use of unialgal enrichment cultures of Prochlorococcus and Synechococcus maintained for hundreds to thousands of generations in the lab. We examine the diversity of heterotrophs in 74 enrichment cultures of these picocyanobacteria obtained from diverse areas of the global oceans.

Results

Heterotroph community composition differed between clades and ecotypes of the autotrophic ‘hosts’ but there was significant overlap in heterotroph community composition across these cultures. Collectively, the cultures were comprised of many shared taxa, even at the genus level. Yet, observed differences in community composition were associated with time since isolation, location, depth, and methods of isolation. The majority of heterotrophs in the cultures are rare in the global ocean, but enrichment conditions favor the opportunistic outgrowth of these rare bacteria. However, we found a few examples, such as bacteria in the family Rhodobacteraceae, of heterotrophs that were ubiquitous and abundant in cultures and in the global oceans. We found their abundance in the wild is also positively correlated with that of picocyanobacteria.

Conclusions

Particular conditions surrounding isolation have a persistent effect on long-term culture composition, likely from bottlenecking and selection that happen during the early stages of enrichment for the picocyanobacteria. We highlight the potential for examining ecologically relevant relationships by identifying patterns of distribution of culture-enriched organisms in the global oceans.
Continuous glucose monitoring in older people with diabetes receiving home care—a feasibility study
Authors: Larsen Annette Bævre , Hermann Monica , Graue Marit
Published: January 2021

Background

Hypoglycemic incidents in older people can cause severe health problems, enhance general age-related disabilities, and cause frailty. Little is known about incidences of hypoglycemia in older home-dwelling people with diabetes. Thus, the aim of this study was to examine the feasibility of capturing hypoglycemia and issues associated with increased risk of hypoglycemia by use of continuous glucose monitoring (CGM) and standardized questionnaires among older home-dwelling individuals with diabetes type 2 receiving home care.

Methods

CGM with the Ipro2-blinded monitoring system were performed for 5 days in six home-dwelling individuals ≥ 75 years diagnosed with diabetes and receiving home care. Demographic (age, gender, living arrangements) and clinical data (diabetes diagnoses and duration, diabetes medication, documented treatment goal, available glycosylated hemoglobin (HbA1c)) were collected from electronic patient records in home care services. Feasibility (ease of use, quality of data, time spent) of standardized questionnaires to identify the risk of hypoglycemia (the McKellar Risk Assessment Tool), risk of malnutrition (the Mini Nutritional Assessment (MNA)), functional status (the Individual-based Statistics for Nursing and Care Services (IPLOS)), and cognitive status (the Mini Mental Status Exam (MMSE)) was also assessed. Questionnaire data was collected by a study nurse in the individuals’ home.

Results

The practical use of CGM was satisfactory, with no major remarks about discomfort or technical errors, except for one participant with skin reaction (redness). Collecting data with the McKellar Risk Assessment Tool, MNA and IPLOS worked well according to quality of data, time spent, and ease of use. The MMSE survey required extensive training of personnel to be conducted.

Conclusion

The feasibility study informs an upcoming study on the incidence and risk factors of hypoglycemia in home-dwelling older individuals. We will ascertain that personnel who will use the MMSE questionnaire to collect cognitive status and skills are familiar with the tool and adequately educated and trained before study start. The use of blinded CGM in this population was well tolerated and can be used “as is” for future studies.
Safety assessment of oral administration of ethanol extract of Justicia carnea leaf in healthy wistar rats: hematology, antioxidative and histology studies
Authors: Akintimehin Emmanuel Sina , Karigidi Kayode Olayele , Omogunwa Tope Samuel , Adetuyi Foluso Olutope
Published: January 2021

Background

Consumption of medicinal plants has diverse therapeutic benefits and could also have toxic effect. Justicia carnea is a medicinal plant that is used conventionally as blood tonic from time immemorial in Nigeria. The aim of this study is to evaluate the safety of ethanol extract of J. carnea leaf assessing the hematology indices, organ antioxidant system and histology in healthy male wistar rats.

Methods

Powdered sample was extracted using absolute ethanol and concentrated to obtain a slurry paste of J. carnea ethanol extracts. Acute toxicity was determined in two phases using Lorke method. In subacute study, rats were randomized into six groups of five rats per group: Group 1 (control) received distilled water, group 2, 3, 4, 5, 6 received 50, 100, 500, 800 and 1200 mg/kg body weight of J. carnea ethanol extract once daily using oral gavage. At the end of 14th day of administration, rats were allowed to fast overnight, sacrificed to collect samples for biochemical analysis and histopathological examination.

Results

The LD50 of extract was greater than 5000 mg/kg body weight. Higher doses (> 500 mg/kg) of extract significantly (p < 0.05) increased RBC, hemoglobin and platelet compared to the control. Liver superoxide dismutase (SOD) activity was significantly (p < 0.05) increased at 1200 mg/kg while other tested doses caused no detrimental effect on glutathione, catalase, SOD and malondialdehyde level in liver and kidney. Histopathological examination of liver and kidney showed mild to severe pathological lesion in a dose dependent manner.

Conclusions

The results of this study suggests that ethanol extract of J. carnea leaf is relatively safe, could be beneficial in alleviating hematology related abnormalities without causing adverse effects on endogenous antioxidant system. However, caution should be taken as higher dose at 1200 mg/kg could cause noticeable tissue injury.
Forecasting and trading cryptocurrencies with machine learning under changing market conditions
Authors: Sebastião Helder , Godinho Pedro
Published: January 2021

This study examines the predictability of three major cryptocurrencies—bitcoin, ethereum, and litecoin—and the profitability of trading strategies devised upon machine learning techniques (e.g., linear models, random forests, and support vector machines). The models are validated in a period characterized by unprecedented turmoil and tested in a period of bear markets, allowing the assessment of whether the predictions are good even when the market direction changes between the validation and test periods. The classification and regression methods use attributes from trading and network activity for the period from August 15, 2015 to March 03, 2019, with the test sample beginning on April 13, 2018. For the test period, five out of 18 individual models have success rates of less than 50%. The trading strategies are built on model assembling. The ensemble assuming that five models produce identical signals (Ensemble 5) achieves the best performance for ethereum and litecoin, with annualized Sharpe ratios of 80.17% and 91.35% and annualized returns (after proportional round-trip trading costs of 0.5%) of 9.62% and 5.73%, respectively. These positive results support the claim that machine learning provides robust techniques for exploring the predictability of cryptocurrencies and for devising profitable trading strategies in these markets, even under adverse market conditions.
European countries with delayed childbearing are not those with lower fertility
Authors: Beaujouan Éva , Toulemon Laurent
Published: January 2021

At the individual level, a very strong negative relationship is observed between age at first birth and total number of children. However, at the country level, it is possible that no relationship exists between factors that reduce fertility at younger ages and stimulate it at older ages. Hence, across countries, the size of the decline in youth fertility is potentially unrelated to the size of the increase in fertility at older ages or the decline in total fertility. We study the fertility of women and their age at childbirth, with particular attention to their evolution over the last 40 years in countries across Europe. Comparing these countries, the increase in births after age 30 has occurred relatively independently of the decline at younger ages according to both period and cohort, and we find no positive relationship between the delay of first birth and decline in total fertility. On the contrary, an inverse relationship evidently exists at the country level, as longer delays generally correspond to smaller declines in total fertility. Context effects largely dominate individual constraints and play an important role in the occurrence of later births.
Use of tracheobronchial tree 3-dimensional printed model: does it improve trainees’ understanding of segmentation anatomy? A prospective study
Authors: O’Brien Christian , Souza Carolina A. , Sheikh Adnan , Miguel Olivier , Wood Timothy
Published: January 2021

Background

This prospective study investigated whether the use of 3D-printed model facilitates novice learning of radiology anatomy on multiplanar computed tomography (CT) when compared to traditional 2D-based learning tools. Specifically, whether the use of a 3D printed model improved interpretation of multiplanar CT tracheobronchial anatomy.

Methods

Thirty-one medical students (10F, 21 M) from years one to three were recruited, matched for gender and level of training and randomized to 2D or 3D group. Students underwent 20-min self-study session using 2D-printed image or 3D-printed model of the tracheobronchial tree. Immediately after, students answered 10 multiple-choice questions (Test 1) to identify tracheobronchial tree branches on multiplanar CT images. Two weeks later, identical test (Test 2) was used to assess retention of information. Mean scores of 2D and 3D groups were calculated. Student’s t test was used to compare mean differences in tests scores and analysis of variance (ANOVA) was used to assess the interaction of gender, CT imaging plane and time on test scores between the two groups.

Results

For test 1, 2D group had higher mean score than 3D group although not statistically significant (7.69 and 7.43, p = 0.39). Mean scores for Test 2 were significantly lower than for Test 1 (7 and 7.57, p = 0.03) with mean score decline for 2D group (Test 1 = 7.69, Test 2 = 6.63, p = 0.03), and similar score for 3D group (Test 1 and 2 = 7.43). There was no statistically significant interaction of gender and test score over time. Significant interaction between group and time of test was found for axial CT images but not for coronal images.

Conclusions

Use of a 3D-printed model of the tracheobronchial anatomy had no immediate advantage over traditional 2D-printed images for learning CT anatomy. However, use of a 3D model improved students’ ability to retain learned information, irrespective of gender.
Correction to: Occurrence of metastatic eccrine porocarcinoma in an immunosuppressed patient
Authors: Modi Mitul B. , Moshiri Ata S. , Shoji Toru , Mihm Martin C. , Xu Xiaowei , Elder David E.
Published: January 2021

An amendment to this paper has been published and can be accessed via the original article.
N- and O-glycan analysis for the detection of glycosylation disorders
Authors: Gouda Amr Sobhi , Elbaz Azza Fahmy , Dupré Thierry , Ali Ola Sayed Mohamed , Zaki Maha Saad , Fateen Ekram Maher
Published: January 2021

Background

Congenital disorders of glycosylation (CDGs) are defined as a group of several rare autosomal recessive inborn errors of metabolism that affect the glycosylation of many proteins and/or lipids. Variable clinical presentation is very characteristic for all types of CDGs; symptoms include severe neurological manifestations that usually start in the neonatal period and cause aggressive irreversible neurological damage. These disorders are usually misdiagnosed as other non-inheritable disorders or remain undiagnosed for a long time, leading to severe neurological complications. The diagnosis of CDGs is quite tedious due to their diverse clinical presentation. In Egypt, there is still no available screening programme to detect CDGs in patients at a young age. Therefore, the need for a reliable rapid test that uses a small sample size has emerged.

This study included 50 suspected subjects and 50 healthy controls with matching age and sex. Western blotting and liquid chromatography-tandem mass spectrometry were used for the analysis of N- and O-glycans, respectively.

Results

The study detected 9 patients with hypoglycosylation (18%). Eight of the nine patients showed abnormal separation of N-glycoproteins using Western blotting indicative of reduced glycosylation (16% of the study subjects and 89% of the subjects with hypoglycosylation). Only one of the nine patients showed a decreased level of sialyl-T-antigen with a normal T-antigen level leading to an increased T/ST ratio (2% of study subjects and 11% of the subjects with hypoglycosylation).

Conclusion

Although N- and O-glycan analysis did not determine the underlying type of CDG, it successfully detected hypoglycosylation in 9 clinically suspected patients (18% of the studied subjects). All detected CDG cases were confirmed by molecular analysis results of mutations causing 4 different types of congenital disorders of glycosylation.
Use of the informational spectrum methodology for rapid biological analysis of the novel coronavirus 2019-nCoV: prediction of potential receptor, natural reservoir, tropism and therapeutic/vaccine target
Authors: Veljkovic Veljko , Vergara-Alert Júlia , Segalés Joaquim , Paessler Slobodan
Published: January 2021

A novel coronavirus recently identified in Wuhan, China (SARS-CoV-2) has expanded the number of highly pathogenic coronaviruses affecting humans. The SARS-CoV-2 represents a potential epidemic or pandemic threat, which requires a quick response for preparedness against this infection. The present report uses the informational spectrum methodology to identify the possible origin and natural host of the new virus, as well as putative therapeutic and vaccine targets. The performed in silico analysis indicates that the newly emerging SARS-CoV-2 is closely related to severe acute respiratory syndrome (SARS)-CoV and, to a lesser degree, Middle East respiratory syndrome (MERS)-CoV. Moreover, the well-known SARS-CoV receptor (ACE2) might be a putative receptor for the novel virus as well. Actin protein was also suggested as a host factor that participates in cell entry and pathogenesis of SARS-CoV-2; therefore, drugs modulating biological activity of this protein (e.g. ibuprofen) were suggested as potential candidates for treatment of this viral infection. Additional results indicated that civets and poultry are potential candidates for the natural reservoir of the SARS-CoV-2, and that domain 288-330 of S1 protein from the SARS-CoV-2 represents promising therapeutic and/or vaccine target.