bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Throughput DNA Metabarcoding of Stem Sections from Trees with Cavities Describes Fungal Communities Associated with Variable Wood Decay, Position on Stem and Tree Species
Authors: Garbelotto Matteo , Johnson Michael G.
Published: May 2023

The presence of unculturable fungi, variability in timing and frequency of fungal fruiting, hyper-rich fungal communities, and genetic and environmental variability explains the difficulty in adopting ideal sampling schemes and fungal identification approaches in studies of fungal communities in wood at variable stages of decay. Here, we use intensive within-tree sampling of five standing trees with cavities paired with high-throughput DNA metabarcoding, to study fungal communities in decayed and healthy wood of trees from two Populus species in British Columbia, Canada. The amplification of over 3000 fungal DNA sequence variants shows the presence of a hyper-rich wood fungal community that not only varied depending on PCR primers, tree species, tree stem portion and wood decay stage. but also included a large number of taxa unassignable to any known sub-kingdom taxonomic order based on published DNA sequences. Our data show that the use of two different primer sets greatly increases the power of the metabarcoding analysis. By testing three alternative models of fungal community composition, we identify the model that best explains fungal community by considering the position on the stem and distance from the cavity. We suggest this model may be used to design optimal sampling schemes to describe fungal communities in trees experiencing discrete decay pockets or cavities.
Evolutionary Processes Shaping Postglacial Gene Pools of High-Altitude Forests: Evidence from the Endemic Eucalypts of Tasmania
Authors: Jones Rebecca C. , Harrison Peter A. , Hudson Corey J. , Hirst Cate A. , Matthews Alexander T. , Rouger Romuald , Wise Sascha L. , O’Reilly-Wapstra Julianne M. , Wiltshire Robert J. E. , Jordan Gregory J. , Vaillancourt René E. , Potts Brad M.
Published: May 2023

Climatic changes during the Pleistocene were responsible for dramatic redistributions of plant species worldwide. On the rugged southern hemisphere island of Tasmania, temperature increases following the last glaciation saw upslope migration of climatically suitable species from lowland refugia and the expansion of eucalypt-dominated forests and woodlands in the Central Highlands. We integrate multiple lines of evidence (chloroplast and nuclear DNA markers, seedling morphology, and survival in common garden experiments) from a group of closely related endemic eucalypts (the alpine white gums) to argue that (i) the Central Highlands of the island were colonised by multiple glacial refugia with hybridisation among species and previously separated populations, and (ii) natural selection has filtered the admixed populations, resulting in local adaptation to the harsh sub-alpine environment. Chloroplast haplotype diversity decreased and nuclear microsatellite diversity increased with altitude, chloroplast sharing among taxa was common, and nuclear DNA differentiation of morphologically distinct taxa was lower in the Central Highlands compared with lowland regions. Local adaptation in the highlands was signalled by evidence from (i) a glasshouse trial in which directional selection (QST > FST) had shaped seedling morphological trait variation and (ii) population survival differences in 35-year-old reciprocal plantings along the major environmental gradients. We conclude that the evolutionary response of these island endemic trees to past climate change has involved the interplay of both hybridisation and natural selection, highlighting the importance of maintaining species interactions under future climate change.
Identification of Structural Differentiation and Differentially Expressed Genes between Sulcus and Culm of Phyllostachys violascens cv. Viridisulcata
Authors: Wei Han-Tian , Vasupalli Naresh , Hou Dan , Pei Jia-Long , Lu Hai-Wen , Wu Ai-Min , Lin Xin-Chun
Published: May 2023

Bamboo is one of the essential ornamental plants that is widely used as a decorative landscape element in gardens. Phyllostachys violascens cv. Viridisulcata has a unique internode color phenotype with yellow culm and green sulcus, but their structural and development differences remain unknown. In the current study, we analyzed the histological analysis of internode cross-sections through SEM and microscopy. These results revealed that the vascular bundles distributed in the culm were organized in oblique rows and multiple lines. In contrast, the vascular bundles’ distribution in the sulcus was much more random. The distribution density, maximum length, and maximum width of vascular bundles were also differentiated between the sulcus and the culm. Further, the cell wall thickness of fiber cells in the culm was more than 30% thicker than the sulcus. The FT-IR analysis identified that the culm and sulcus had similar structural properties. The total lignin content measurement revealed that lignin accumulated more in the sulcus than in the culm. Additionally, we identified the lignin biosynthesis pathway genes, Pv4CL and PvC4H, which were differentially expressed between the culm and sulcus through transcriptomic data and qPCR analyses. In conclusion, our results identified that the vascular bundles’ structure differed between the culm and sulcus, and Pv4CL and PvC4H genes might play an essential role in their development.
Use of Functional Traits to Distinguish Successional Guilds of Tree Species for Restoring Forest Ecosystems
Authors: Manohan Benjapan , Shannon Dia Panitnard , Tiansawat Pimonrat , Chairuangsri Sutthathorn , Jainuan Jutatip , Elliott Stephen
Published: May 2023

Forest ecosystem restoration involves establishing mixes of tree species representing various successional stages of the reference forest. When selecting species, conceptualizing successional status as a gradient of guilds is more appropriate than the conventional binary classification of pioneer and climax species. Therefore, we tested the hypothesis that functional traits can be used to distinguish successional guilds among tree species, planted to test the framework species method of restoration. Values of 13 non-intercorrelated traits of 28 species, derived from field measurements and databases, were analyzed by cluster analysis and rank scoring. Cluster analysis grouped species into six guilds. For rank scoring, negative (from 0 to −2) and positive scores (from 0 to +2) were assigned to each trait, according to their association with early or late succession, respectively. Seven guilds were distinguished from the total scores. This novel technique placed species evenly along a gradient, with 13 and 15 species attaining negative and positive total scores, respectively. Cross-validation between the two techniques was high, signifying the robustness of using functional traits to distinguish successional guilds. Functional traits, therefore, provide a powerful tool to inform species selection when planning forest restoration. However, their wider use depends on greater availability of functional trait data for more tree species.
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Using Pichia pastoris in Stirred-Tank Bioreactor
Authors: Noseda Diego G. , D’Alessio Cecilia , Santos Javier , Idrovo-Hidalgo Tommy , Pignataro Florencia , Wetzler Diana E. , Gentili Hernán , Nadra Alejandro D. , Roman Ernesto , Paván Carlos , Ruberto Lucas A. M.
Published: May 2023

SARS-CoV-2 was identified as the pathogenic agent causing the COVID-19 pandemic. Among the proteins codified by this virus, the Spike protein is one of the most-external and -exposed. A fragment of the Spike protein, named the receptor binding domain (RBD), interacts with the ACE2 receptors of human cells, allowing the entrance of the viruses. RBD has been proposed as an interesting protein for the development of diagnosis tools, treatment, and prevention of the disease. In this work, a method for recombinant RBD production using Pichia pastoris as a cell factory in a stirred-tank bioreactor (SRTB) up to 7 L was developed. Using a basal saline medium with glycerol, methanol, and compressed air in a four-stage procedure, around 500 mg/L of the raw RBD produced by yeasts (yRBD) and 206 mg/L of purified (>95%) RBD were obtained. Thereby, the proposed method represents a feasible, simple, scalable, and inexpensive procedure for the obtention of RBD for diagnosis kits and vaccines’ formulation.
Development and Validation of a 54K Genome-Wide Liquid SNP Chip Panel by Target Sequencing for Dairy Goat
Authors: Guan Shengyu , Li Weining , Jin Hai , Zhang Lu , Liu Guoshi
Published: May 2023

As an important genotyping platform, SNP chips are essential for implementing genomic selection. In this article, we introduced the development of a liquid SNP chip panel for dairy goats. This panel contains 54,188 SNPs based on genotyping by targeted sequencing (GBTS) technology. The source of SNPs in the panel were from the whole-genome resequencing of 110 dairy goats from three European and two Chinese indigenous dairy goat breeds. The performance of this liquid SNP chip panel was evaluated by genotyping 200 additional goats. Fifteen of them were randomly selected for whole-genome resequencing. The average capture ratio of the panel design loci was 98.41%, and the genotype concordance with resequencing reached 98.02%. We further used this chip panel to conduct genome-wide association studies (GWAS) to detect genetic loci that affect coat color in dairy goats. A single significant association signal for hair color was found on chromosome 8 at 31.52–35.02 Mb. The TYRP1 gene, which is associated with coat color in goats, was identified to be located at this genomic region (chromosome 8: 31,500,048-31,519,064). The emergence of high-precision and low-cost liquid microarrays will improve the analysis of genomics and breeding efficiency of dairy goats.
Characterization of the Liriodendron chinense Pentatricopeptide Repeat (PPR) Gene Family and Its Role in Osmotic Stress Response
Authors: Ma Xiaoxiao , Wang Dandan , Xue Guoxia , Zheng Xueyan , Lu Ye , Shi Jisen , Hao Zhaodong , Chen Jinhui
Published: May 2023

The Pentatricopeptide repeat (PPR) superfamily is a large gene family in plants that regulates organelle RNA metabolism, which is important for plant growth and development. However, a genome-wide analysis of the PPR gene family and its response to abiotic stress has not been reported for the relict woody plant Liriodendron chinense. In this paper, we identified 650 PPR genes from the L. chinense genome. A phylogenetic analysis showed that the LcPPR genes could roughly be divided into the P and PLS subfamilies. We found that 598 LcPPR genes were widely distributed across 19 chromosomes. An intraspecies synteny analysis indicated that duplicated genes from segmental duplication contributed to the expansion of the LcPPR gene family in the L. chinense genome. In addition, we verified the relative expression of Lchi03277, Lchi06624, Lchi18566, and Lchi23489 in the roots, stems, and leaves and found that all four genes had the highest expression in the leaves. By simulating a drought treatment and quantitative reverse transcription PCR (qRT-PCR) analysis, we confirmed the drought-responsive transcriptional changes in four LcPPR genes, two of which responded to drought stress independent of endogenous ABA biosynthesis. Thus, our study provides a comprehensive analysis of the L. chinense PPR gene family. It contributes to research into their roles in this valuable tree species’ growth, development, and stress resistance.
DNA Barcodes of Mansonia (Mansonia) Blanchard, 1901 (Diptera, Culicidae)
Authors: Amorim Jandui Almeida , de Oliveira Tatiane Marques Porangaba , de Sá Ivy Luizi Rodrigues , da Silva Taires Peniche , Sallum Maria Anice Mureb
Published: May 2023

Females of the genus Mansonia feed on the blood of humans, livestock, and other vertebrates to develop their eggs. The females’ biting behavior may cause severe disturbance to blood hosts, with a negative impact on public health and economics. Certain species have been identified as potential or effective disease vectors. The accurate species identification of field-collected specimens is of paramount importance for the success of monitoring and control strategies. Mansonia (Mansonia) morphological species boundaries are blurred by patterns of intraspecific heteromorphism and interspecific isomorphism. DNA barcodes can help to solve taxonomic controversies, especially if combined with other molecular tools. We used cytochrome c oxidase subunit I (COI) gene 5′ end (DNA barcode) sequences to identify 327 field-collected specimens of Mansonia (Mansonia) spp. The sampling encompassed males and females collected from three Brazilian regions and previously assigned to species based on their morphological characteristics. Eleven GenBank and BOLD sequences were added to the DNA barcode analyses. Initial morphospecies assignments were mostly corroborated by the results of five clustering methods based on Kimura two-parameter distance and maximum likelihood phylogeny. Five to eight molecular operational taxonomic units may represent taxonomically unknown species. The first DNA barcode records for Mansonia fonsecai, Mansonia iguassuensis, and Mansonia pseudotitillans are presented.
Investigation of Resistance Genes in Genus Vigna Reveals Highly Variable NLRome in Parallel Domesticated Member Species
Authors: Asif Jehanzaib , Qureshi Fatima , Zain Muhammad , Nawaz Hamza , Naz Effat , Fareed Shahid , Bibi Aqsa , Nawaz Sehar , Saleem Fozia , Shafique Muhammad , Tabasum Saba , Maqsood Umer , Serfraz Saad , Alkahtani Saad
Published: May 2023

Vigna is a unique genus that consist of multiple crop species that are domesticated in parallel fashion between 7–10 thousand years ago. Here we studied the evolution of nucleotide-binding site leucine-rich repeat receptor (NLR) genes across five crop species of genus Vigna. In total identified 286, 350, 234, 250, 108 and 161 NLR genes were from Phaseolous vulgaris, Vigna. unguiculata, Vigna mungo, Vigna radiata, Vigna angularis and Vigna umbellata respectively. Comprehensive phylogenetic and clusterization analysis reveals the presence of seven subgroups of Coiled coil like NLRs (CC-NLR) genes and four distinct lineages of Toll interleukin receptor like NLRs (TIR-NLR). Subgroup CCG10-NLR shows large scale diversification among Vigna species suggesting genus specific distinct duplication pattern in Vigna species. Mainly birth of new NLR gene families and higher rate of terminal duplication is the major determinants for expansion of NLRome in genus Vigna. Recent expansion of NLRome in V. anguiculata and V. radiata was also observed which might suggest that domestication have supported their duplication of lineage specific NLR genes. In short, large scale difference in the architecture of NLRome were observed in diploid plant species. Our findings allowed us to hypothesized that independent parallel domestication is the major drivers of highly divergent evolution of NLRome in genus Vigna.
A Pervasive History of Gene Flow in Madagascar’s True Lemurs (Genus Eulemur)
Authors: Everson Kathryn M. , Donohue Mariah E. , Weisrock David W.
Published: May 2023

In recent years, it has become widely accepted that interspecific gene flow is common across the Tree of Life. Questions remain about how species boundaries can be maintained in the face of high levels of gene flow and how phylogeneticists should account for reticulation in their analyses. The true lemurs of Madagascar (genus Eulemur, 12 species) provide a unique opportunity to explore these questions, as they form a recent radiation with at least five active hybrid zones. Here, we present new analyses of a mitochondrial dataset with hundreds of individuals in the genus Eulemur, as well as a nuclear dataset containing hundreds of genetic loci for a small number of individuals. Traditional coalescent-based phylogenetic analyses of both datasets reveal that not all recognized species are monophyletic. Using network-based approaches, we also find that a species tree containing between one and three ancient reticulations is supported by strong evidence. Together, these results suggest that hybridization has been a prominent feature of the genus Eulemur in both the past and present. We also recommend that greater taxonomic attention should be paid to this group so that geographic boundaries and conservation priorities can be better established.
Genome-Wide Integrative Transcriptional Profiling Identifies Age-Associated Signatures in Dogs
Authors: Kim Hyun Seung , Jang Subin , Kim Jaemin
Published: May 2023

Mammals experience similar stages of embryonic development, birth, infancy, youth, adolescence, maturity, and senescence. While embryonic developmental processes have been extensively researched, many molecular mechanisms regulating the different life stages after birth, such as aging, remain unresolved. We investigated the conserved and global molecular transitions in transcriptional remodeling with age in dogs of 15 breeds, which revealed that genes underlying hormone level regulation and developmental programs were differentially regulated during aging. Subsequently, we show that the candidate genes associated with tumorigenesis also exhibit age-dependent DNA methylation patterns, which might have contributed to the tumor state through inhibiting the plasticity of cell differentiation processes during aging, and ultimately suggesting the molecular events that link the processes of aging and cancer. These results highlight that the rate of age-related transcriptional remodeling is influenced not only by the lifespan, but also by the timing of critical physiological milestones.
Challenges of Integrating New Technologies for Orthopedic Doctors to Face up to Difficulties during the Pandemic Era
Authors: Niculescu Marius , Honțaru Octavia-Sorina , Popescu George , Sterian Alin Gabriel , Dobra Mihai
Published: May 2023

In the field of orthopedics, competitive progress is growing faster because new technologies used to facilitate the work of physicians are continuously developing. Based on the issues generated in the pandemic era in this field, a research study was developed to identify the intention of orthopedic doctors to integrate new medical technologies. The survey was based on a questionnaire that was used for data collection. The quantitative study registered a sample of 145 orthopedic doctors. The data analysis was performed based on the IBM SPSS program. A multiple linear regression model was applied, which analyzed how the independent variables can influence the dependent variables. After analyzing the data, it was observed that the intention of orthopedic doctors to use new medical technologies is influenced by the advantages and disadvantages perceived by them, the perceived risks, the quality of the medical technologies, the experience of physicians in their use, and their receptivity to other digital tools. The obtained results are highly important both for hospital managers and authorities, illustrating the main factors that influence doctors to use emergent technologies in their clinical work.
Mandarin Essential Oils as an Alternative Method of Controlling the Fungus Alternaria alternata (Fr.: Fr.) Keissler
Authors: Devite Fernando Trevizan , Azevedo Fernando Alves de , Bastianel Marinês , Schinor Evandro Henrique , Conceição Patrícia Marluci da
Published: May 2023

Alternaria brown spot (ABS) is a disease caused by the fungus A. alternata f. sp. citri, which results in lesions on the fruits, leaves, and branches of several mandarin varieties and their hybrids. Due to the high cost of fungicide application, alternative methods for controlling ABS need to be studied. Therefore, this study aimed to evaluate the use of essential oils (EOs) from different mandarin varieties to mitigate the effects of ABS. The inhibitory effect of different concentrations (1, 2, 4, 8, and 16 μL·mL−1) of the EOs of Fremont IAC 543 mandarin, IAC 2019Maria mandarin, Murcott IAC 221 tangor, and Late IAC 855 willowleaf on the in vitro mycelial growth of the fungus A. alternata was evaluated. Additionally, the curative and preventive effects of these EOs on the ABS symptoms in detached leaves of Murcott IAC 221 tangor were also assessed. The EO of IAC 2019 Maria mandarin induced less mycelial growth, and consequently, a greater inhibition of the growth of the fungus A. alternata at a concentration of 16 μL·mL−1. This EO was more effective for control than the other oils tested. In the detached leaf experiment, both the curative and preventive treatments at a concentration of 16 μL·mL−1 showed lower values of disease severity.
Stress Response Analysis via Dynamic Entropy in EEG: Caregivers in View
Authors: Zavala-Yoé Ricardo , Iqbal Hafiz M. N. , Parra-Saldívar Roberto , Ramírez-Mendoza Ricardo A.
Published: May 2023

According to the World Health Organization (WHO), stress can be defined as any type of alteration that causes physical, emotional, or psychological tension. A very important concept that is sometimes confused with stress is anxiety. The difference between stress and anxiety is that stress usually has an existing cause. Once that activator has passed, stress typically eases. In this respect, according to the American Psychiatric Association, anxiety is a normal response to stress and can even be advantageous in some circumstances. By contrast, anxiety disorders differ from temporary feelings of anxiousness or nervousness with more intense feelings of fear or anxiety. The Diagnostic and Statistical Manual (DSM-5) explicitly describes anxiety as exorbitant concern and fearful expectations, occurring on most days for at least 6 months, about a series of events. Stress can be measured by some standardized questionnaires; however, these resources are characterized by some major disadvantages, the main one being the time consumed to interpret them; i.e., qualitative information must be transformed to quantitative data. Conversely, a physiological recourse has the advantage that it provides quantitative spatiotemporal information directly from brain areas and it processes data faster than qualitative supplies. A typical option for this is an electroencephalographic record (EEG). We propose, as a novelty, the application of time series (TS) entropies developed by us to inspect collections of EEGs obtained during stress situations. We investigated this database related to 23 persons, with 1920 samples (15 s) captured in 14 channels for 12 stressful events. Our parameters reflected that out of 12 events, event 2 (Family/financial instability/maltreatment) and 10 (Fear of disease and missing an important event) created more tension than the others. In addition, the most active lobes reflected by the EEG channels were frontal and temporal. The former is in charge of performing higher functions, self-control, self monitoring, and the latter is in charge of auditory processing, but also emotional handling. Thus, events E2 and E10 triggering frontal and temporal channels revealed the actual state of participants under stressful situations. The coefficient of variation revealed that E7 (Fear of getting cheated/losing someone) and E11 (Fear of suffering a serious illness) were the events with more changes among participants. In the same sense, AF4, FC5, and F7 (mainly frontal lobe channels) were the most irregular on average for all participants. In summary, by means of dynamic entropy analysis, the goal is to process the EEG dataset in order to elucidate which event and brain regions are key for all participants. The latter will allow us to easily determine which was the most stressful and on which brain zone. This study can be applied to other caregivers datasets. All this is a novelty.
ResQ Family: Respiratory Syncytial Virus (RSV) Infection in Infants and Quality of Life of Families—Study Protocol of a Multi-Country Family Cohort Study
Authors: Trautmannsberger Ilona , Bösl Sabina , Tischer Christina , Kostenzer Johanna , Mader Silke , Zimmermann Luc J. I. ,   the ResQ Family Study Group
Published: May 2023

(1) Respiratory syncytial virus (RSV) infection in infants not only affects the child itself, but also their families. Nevertheless, information on the overall impact is scarce. A comprehensive caregiver-specific approach covering essential (health) dimensions and relevant stakeholders was initiated under the ResQ Family study conducted in Germany, France, Italy and Sweden. The primary objective is to investigate the health-related quality of life of parents and/or caregivers of children (<24 months) hospitalised for RSV. (2) Each participant completes an online questionnaire disseminated via social media and printed material in hospitals. Using the PedsQLTM FIM as well as further self-designed questions, parent and patient characteristics as well as potential stressors and preventive factors are recorded at baseline and after six weeks. Multivariate regression models with health-related quality of life as main outcome parameter will be conducted. (3) The study is currently in the recruitment process. A full analysis will be performed once the data collection phase is complete. First results are to be expected in late 2023. (4) Publishing the results in the form of scientific papers but also non-scientific (information) material will help us raise awareness for RSV and the importance of prevention among healthcare professionals, patient representatives and decision-makers.
Leveraging the Expertise of the Community: A Case for Expansion of a Peer Workforce in Child, Adolescent, and Family Mental Health
Authors: Robertson Hillary A. , Biel Matthew G. , Hayes Katherine R. , Snowden Sara , Curtis Latisha , Charlot-Swilley Dominique , Clauson Elyssa S. , Gavins Arrealia , Sisk Caslin M. , Bravo Noel , Coates Erica E. , Domitrovich Celene E.
Published: May 2023

The rise in child and adolescent mental health concerns has led to the need for an expanded workforce to meet the needs of our nation’s families. Peer paraprofessionals (PPs) have proven to be impactful in the areas of adult mental health (MH) and substance use disorders, and for persons with chronic medical conditions. PPs can contribute to addressing child, adolescent, and family MH needs by being deployed in community settings and providing both emotional and tangible support to families and children. Additional use of PPs can address equity gaps in MH services by improving access to support and enhancing the cultural acceptability of MH interventions. A concentrated effort to expand and develop this workforce may help to alleviate the strain on the current MH system. The Georgetown University Infant and Early Childhood Certificate program is a paraprofessional training program that prepares community members to meet the MH needs of families with young children. The authors will describe the results of a qualitative study examining the landscape of peer paraprofessional services in DC that was conducted to support the expansion of the peer workforce to include individuals with expertise in infant and early childhood mental health.
Age-Related Differences in Anxiety and Depression Diagnosis among Adults in Puerto Rico during the COVID-19 Pandemic
Authors: Cameron-Maldonado Stephanie , Pérez Cynthia M. , Fernández-Repollet Emma , López-Cepero Andrea
Published: May 2023

Residents of Puerto Rico bear a significant burden of mental health disorders, which the COVID-19 pandemic may have exacerbated. However, age-specific data on these disorders during the pandemic in Puerto Rico are scarce. This study evaluated age-related differences in the self-reported diagnosis of depression and anxiety among adults ≥18 years residing in Puerto Rico during the pandemic. An anonymous online survey was administered from December 2020 to February 2021 via Google Forms to measure self-reported sociodemographic and behavioral characteristics and physician-diagnosed mental health disorders. Multivariable logistic regression models were conducted for each self-reported mental health diagnosis after adjusting for sex, education, income, marital status, chronic diseases, and smoking. Out of 1945 adults, 50% were aged 40 years and over. Nearly 24% of responders self-reported an anxiety diagnosis, whereas 15.9% reported depression. Compared to individuals 50 years and over, those 18–29 y, 30–39 y, and 40–49 y had significantly higher odds of an anxiety diagnosis (OR = 1.84, 95% CI = 1.34–2.55; OR = 1.50, 95% CI = 1.09–2.07; and OR = 1.37, 95% CI = 1.01–1.87, respectively). However, no association between age and depression diagnosis was found. Despite anxiety and depression being frequent disorders during the pandemic in this sample, younger adults bear a higher burden of anxiety. Further research is needed to allocate appropriate mental health resources during emergencies according to population subgroups.
Disaster Mental Health Risk Reduction: Appraising Disaster Mental Health Research as If Risk Mattered
Authors: Reifels Lennart , Dückers Michel L. A.
Published: May 2023

The globally increasing frequency, intensity, and complexity of extreme climatic events and disasters poses significant challenges for the future health and wellbeing of affected populations around the world [...]
Analysis of University Student Motivation in Cross-Border Contexts
Authors: Sánchez-Bolívar Lionel , Navarro-Prado Silvia , Sánchez-Ojeda María Angustias , García-Morales Victoria , Cortés-Martín Jonathan , Tovar-Gálvez María Isabel
Published: May 2023

The development of the personality of university students can determine their affinities for certain disciplines; therefore, it is important to know their specific socio-demographic and motivational profile, what motivates them to start a certain university degree and what encourages them to continue with it, which can help to adapt the teaching methodology. A total of 292 university students from the University of Granada (Ceuta and Melilla campuses) participated in this quantitative study with a descriptive, cross-sectional design, in which motivation and social skills were analysed. Among the results, it can be highlighted that the student population is mainly female, with a higher level of motivation. Sociability, communication, thinking (optimistic or pessimistic), empathy and self-confidence are skills that affect university students’ motivation levels. This study highlights the importance and impact of students’ motivation on their learning and the development of their social competence, so it is essential to carry out educational interventions that promote these types of skills, especially in cross-border contexts, which can be demotivating environments.
Coping Strategies as a Moderator for the Association between Intimate Partner Violence and Depression and Anxiety Symptoms among Transgender Women
Authors: Gray Shannon S. , Sizemore Kayla Marie , Rendina H. Jonathon
Published: May 2023

Evidence suggests that intimate partner violence (IPV) is associated with negative mental health outcomes. There is currently limited research on the impact of IPV on the outcomes of mental health for transgender women. The current study aimed to examine the relationship between intimate partner violence, coping skills, depression, and anxiety in a sample of transgender women. Hierarchical regression analyses were conducted examining the relationship of IPV and depression and anxiety symptoms, where coping skills moderate this relationship. The results suggest that those with experiences of IPV are more likely to have symptoms of depression and anxiety. For individuals with no experiences of IPV and low depression, high levels of emotional processing coping and acceptance coping buffered this relationship. For individuals with more experiences of IPV and more depressive symptoms, coping skills did not show to buffer this relationship. These same coping skills did not show evidence for buffering anxiety symptoms for transgender women with low or high levels of IPV. The results, implications, and limitations of this study and suggestions for further research are discussed.
RNAi Technology: A New Path for the Research and Management of Obligate Biotrophic Phytopathogenic Fungi
Authors: Padilla-Roji Isabel , Ruiz-Jiménez Laura , Bakhat Nisrine , Vielba-Fernández Alejandra , Pérez-García Alejandro , Fernández-Ortuño Dolores
Published: May 2023

Powdery mildew and rust fungi are major agricultural problems affecting many economically important crops and causing significant yield losses. These fungi are obligate biotrophic parasites that are completely dependent on their hosts for growth and reproduction. Biotrophy in these fungi is determined by the presence of haustoria, specialized fungal cells that are responsible for nutrient uptake and molecular dialogue with the host, a fact that undoubtedly complicates their study under laboratory conditions, especially in terms of genetic manipulation. RNA interference (RNAi) is the biological process of suppressing the expression of a target gene through double-stranded RNA that induces mRNA degradation. RNAi technology has revolutionized the study of these obligate biotrophic fungi by enabling the analysis of gene function in these fungal. More importantly, RNAi technology has opened new perspectives for the management of powdery mildew and rust diseases, first through the stable expression of RNAi constructs in transgenic plants and, more recently, through the non-transgenic approach called spray-induced gene silencing (SIGS). In this review, the impact of RNAi technology on the research and management of powdery mildew and rust fungi will be addressed.
Parkinson’s Disease: Exploring Different Animal Model Systems
Authors: Khan Engila , Hasan Ikramul , Haque M. Emdadul
Published: May 2023

Disease modeling in non-human subjects is an essential part of any clinical research. To gain proper understanding of the etiology and pathophysiology of any disease, experimental models are required to replicate the disease process. Due to the huge diversity in pathophysiology and prognosis in different diseases, animal modeling is customized and specific accordingly. As in other neurodegenerative diseases, Parkinson’s disease is a progressive disorder coupled with varying forms of physical and mental disabilities. The pathological hallmarks of Parkinson’s disease are associated with the accumulation of misfolded protein called α-synuclein as Lewy body, and degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc) area affecting the patient’s motor activity. Extensive research has already been conducted regarding animal modeling of Parkinson’s diseases. These include animal systems with induction of Parkinson’s, either pharmacologically or via genetic manipulation. In this review, we will be summarizing and discussing some of the commonly employed Parkinson’s disease animal model systems and their applications and limitations.
Single-Cell RNA-Seq Analysis Reveals Ferroptosis in the Tumor Microenvironment of Clear Cell Renal Cell Carcinoma
Authors: Zhang Jing , Deng Yun , Zhang Hui , Zhang Zhiyuan , Jin Xin , Xuan Yan , Zhang Zhen , Ma Xuejun
Published: May 2023

In this study, we investigated the role of ferroptosis in the tumor microenvironment (TME) of clear cell renal cell carcinoma (ccRCC), the leading cause of renal cancer-related death. We analyzed single-cell data from seven ccRCC cases to determine cell types most correlated with ferroptosis and performed pseudotime analysis on three myeloid subtypes. We identified 16 immune-related ferroptosis genes (IRFGs) by analyzing differentially expressed genes between cell subgroups and between high and low immune infiltration groups in the TCGA-KIRC dataset and the FerrDb V2 database. Using univariate and multivariate Cox regression, we identified two independent prognostic genes, AMN and PDK4, and constructed an IRFG score model immune-related ferroptosis genes risk score (IRFGRs) to evaluate its prognostic value in ccRCC. The IRFGRs demonstrated excellent and stable performance for predicting ccRCC patient survival in both the TCGA training set and the ArrayExpress validation set, with an AUC range of 0.690–0.754, outperforming other commonly used clinicopathological indicators. Our findings enhance the understanding of TME infiltration with ferroptosis and identify immune-mediated ferroptosis genes associated with prognosis in ccRCC.
Unveiling the Stereoselectivity and Regioselectivity of the [3+2] Cycloaddition Reaction between N-methyl-C-4-methylphenyl-nitrone and 2-Propynamide from a MEDT Perspective
Authors: Salih Sabir A. Mohammed , Basheer Huda A. , de Julián-Ortiz Jesus Vicente , Mohammad-Salim Haydar A.
Published: May 2023

[3+2] cycloaddition reactions play a crucial role in synthesizing complex organic molecules and have significant applications in drug discovery and materials science. In this study, the [3+2] cycloaddition (32CA) reactions of N-methyl-C-4-methyl phenyl-nitrone 1 and 2-propynamide 2, which have not been extensively studied before, were investigated using molecular electron density theory (MEDT) at the B3LYP/6–311++G(d,p) level of theory. According to an electron localization function (ELF) study, N-methyl-C-4-methyl phenyl-nitrone 1 is a zwitterionic species with no pseudoradical or carbenoid centers. Conceptual density functional theory (CDFT) indices were used to predict the global electronic flux from the strong nucleophilic N-methyl-C-4-methyl phenylnitrone 1 to the electrophilic 2-propynamide 2 functions. The 32CA reactions proceeded through two pairs of stereo- and regioisomeric reaction pathways to generate four different products: 3, 4, 5, and 6. The reaction pathways were irreversible owing to their exothermic characters: −136.48, −130.08, −130.99, and −140.81 kJ mol−1, respectively. The enthalpy of the 32CA reaction leading to the formation of cycloadduct 6 was lower compared with the other path owing to a slight increase in its polar character, observed through the global electron density transfer (GEDT) during the transition states and along the reaction path. A bonding evolution theory (BET) analysis showed that these 32CA reactions proceed through the coupling of pseudoradical centers, and the formation of new C-C and C-O covalent bonds did not begin in the transition states.
Kidney-Related Function of Mitochondrial Protein Mitoregulin
Authors: Averina Olga A. , Permyakov Oleg A. , Emelianova Mariia A. , Guseva Ekaterina A. , Grigoryeva Olga O. , Lovat Maxim L. , Egorova Anna E. , Grinchenko Andrei V. , Kumeiko Vadim V. , Marey Maria V. , Manskikh Vasily N. , Dontsova Olga A. , Vyssokikh Mikhail Y. , Sergiev Petr V.
Published: May 2023

A small protein, Mitoregulin (Mtln), localizes in mitochondria and contributes to oxidative phosphorylation and fatty acid metabolism. Mtln knockout mice develop obesity on a high-fat diet, demonstrating elevated cardiolipin damage and suboptimal creatine kinase oligomerization in muscle tissue. Kidneys heavily depend on the oxidative phosphorylation in mitochondria. Here we report kidney-related phenotypes in aged Mtln knockout mice. Similar to Mtln knockout mice muscle mitochondria, those of the kidney demonstrate a decreased respiratory complex I activity and excessive cardiolipin damage. Aged male mice carrying Mtln knockout demonstrated an increased frequency of renal proximal tubules’ degeneration. At the same time, a decreased glomerular filtration rate has been more frequently detected in aged female mice devoid of Mtln. An amount of Mtln partner protein, Cyb5r3, is drastically decreased in the kidneys of Mtln knockout mice.
Study of the Electronic Interaction between NiO and Short Polythiophene Chains towards Solar Photon Harvesting
Authors: Carbajal-Franco Guillermo , Márquez-Quintana María Fernanda , Rojas-Chávez Hugo , Miralrio Alan
Published: May 2023

The sustainable production of energy is a field of interest to which a new requirement is now imposed: the need to be respectful of the environment. New materials and techniques are being developed, but environmental concerns impose the necessity of keeping research active towards the development of green energy. For this reason, we present the study of short polythiophene (PTh) chains (three and five monomers) and their interaction with nickel oxide, looking for properties related to solar photon harvesting in order to produce electricity. The models of the molecules were developed, and the calculations were performed with an M11-L meta-GGA functional, specially developed for electronic structure calculations. The theoretical explorations demonstrated that the geometry of the PTh molecules suffer little distortion when interacting with the NiO molecule. The calculated value of Eg lies between 2.500 and 0.412 eV for a three-ring PTh chain and between 1.944 and 0.556 eV for a five-ring PTh chain. The chemical parameters indicated that, depending on the geometry of the system, the chemical potential varies from 81.27 to 102.38 kcal/mol and the highest amount of electronic charge varies from −2.94 to 21.56 a.u. for three-monomer systems. For five-monomer systems, the values lie within similar ranges as those of the three-monomer systems. The Partial Density of States (PDOS) showed that the valence and conduction electronic bands were composed of states in the NiO and PTh rings, except for a system where there was a non-bonding interaction.
Effect of Hepatic Pathology on Liver Regeneration: The Main Metabolic Mechanisms Causing Impaired Hepatic Regeneration
Authors: Rodimova Svetlana , Mozherov Artem , Elagin Vadim , Karabut Maria , Shchechkin Ilya , Kozlov Dmitry , Krylov Dmitry , Gavrina Alena , Bobrov Nikolai , Zagainov Vladimir , Zagaynova Elena , Kuznetsova Daria
Published: May 2023

Liver regeneration has been studied for many decades, and the mechanisms underlying regeneration of normal liver following resection are well described. However, no less relevant is the study of mechanisms that disrupt the process of liver regeneration. First of all, a violation of liver regeneration can occur in the presence of concomitant hepatic pathology, which is a key factor reducing the liver’s regenerative potential. Understanding these mechanisms could enable the rational targeting of specific therapies to either reduce the factors inhibiting regeneration or to directly stimulate liver regeneration. This review describes the known mechanisms of normal liver regeneration and factors that reduce its regenerative potential, primarily at the level of hepatocyte metabolism, in the presence of concomitant hepatic pathology. We also briefly discuss promising strategies for stimulating liver regeneration and those concerning methods for assessing the regenerative potential of the liver, especially intraoperatively.
Strategies for Reducing Toxicity and Enhancing Efficacy of Chimeric Antigen Receptor T Cell Therapy in Hematological Malignancies
Authors: Wang Haobing , Tang Ling , Kong Yingjie , Liu Wen , Zhu Xiaojian , You Yong
Published: May 2023

Chimeric antigen receptor T cell (CAR-T) therapy in hematologic malignancies has made great progress, but there are still some problems. First, T cells from tumor patients show an exhaustion phenotype; thus, the persistence and function of the CAR-Ts are poor, and achieving a satisfactory curative effect is difficult. Second, some patients initially respond well but quickly develop antigen-negative tumor recurrence. Thirdly, CAR-T treatment is not effective in some patients and is accompanied by severe side effects, such as cytokine release syndrome (CRS) and neurotoxicity. The solution to these problems is to reduce the toxicity and enhance the efficacy of CAR-T therapy. In this paper, we describe various strategies for reducing the toxicity and enhancing the efficacy of CAR-T therapy in hematological malignancies. In the first section, strategies for modifying CAR-Ts using gene-editing technologies or combining them with other anti-tumor drugs to enhance the efficacy of CAR-T therapy are introduced. The second section describes some methods in which the design and construction of CAR-Ts differ from the conventional process. The aim of these methods is to enhance the anti-tumor activity of CAR-Ts and prevent tumor recurrence. The third section describes modifying the CAR structure or installing safety switches to radically reduce CAR-T toxicity or regulating inflammatory cytokines to control the symptoms of CAR-T-associated toxicity. Together, the knowledge summarized herein will aid in designing better-suited and safer CAR-T treatment strategies.
Characterization and Diversity of Klebsiella pneumoniae Prophages
Authors: Kang Fuqiang , Chai Zili , Li Beiping , Hu Mingda , Yang Zilong , Wang Xia , Liu Wenting , Ren Hongguang , Jin Yuan , Yue Junjie
Published: May 2023

Klebsiella pneumoniae is a common human commensal and opportunistic pathogen. In recent years, the clinical isolation and resistance rates of K. pneumoniae have shown a yearly increase, leading to a special interest in mobile genetic elements. Prophages are a representative class of mobile genetic elements that can carry host-friendly genes, transfer horizontally between strains, and coevolve with the host’s genome. In this study, we identified 15,946 prophages from the genomes of 1437 fully assembled K. pneumoniae deposited in the NCBI database, with 9755 prophages on chromosomes and 6191 prophages on plasmids. We found prophages to be notably diverse and widely disseminated in the K. pneumoniae genomes. The K. pneumoniae prophages encoded multiple putative virulence factors and antibiotic resistance genes. The comparison of strain types with prophage types suggests that the two may be related. The differences in GC content between the same type of prophages and the genomic region in which they were located indicates the alien properties of the prophages. The overall distribution of GC content suggests that prophages integrated on chromosomes and plasmids may have different evolutionary characteristics. These results suggest a high prevalence of prophages in the K. pneumoniae genome and highlight the effect of prophages on strain characterization.
Comparison between Capillary and Serum Lactate Levels in Predicting Short-Term Mortality of Septic Patients at the Emergency Department
Authors: Guarino Matteo , Perna Benedetta , Cesaro Alice Eleonora , Spampinato Michele Domenico , Previati Rita , Costanzini Anna , Maritati Martina , Contini Carlo , De Giorgio Roberto
Published: May 2023

Sepsis is a time-dependent and life-threating condition related to macro- and micro-circulatory impairment leading to anaerobic metabolism and lactate increase. We assessed the prognostic accuracy of capillary lactates (CLs) vs. serum ones (SLs) on 48-h and 7-day mortality in patients with suspected sepsis. This observational, prospective, single-centre study was conducted between October 2021 and May 2022. Inclusion criteria were: (i) suspect of infection; (ii) qSOFA ≥ 2; (iii) age ≥ 18 years; (iv) signed informed consent. CLs were assessed with LactateProTM2®. 203 patients were included: 19 (9.3%) died within 48 h from admission to the Emergency Department, while 28 (13.8%) within 7 days. Patients deceased within 48 h (vs. survived) had higher CLs (19.3 vs. 5 mmol/L, p < 0.001) and SLs (6.5 vs. 1.1 mmol/L, p = 0.001). The best CLs predictive cut-off for 48-h mortality was 16.8 mmol/L (72.22% sensitivity, 94.02% specificity). Patients within 7 days had higher CLs (11.5 vs. 5 mmol/L, p = 0.020) than SLs (2.75 vs. 1.1 mmol/L, p < 0.001). The multivariate analysis confirmed CLs and SLs as independent predictors of 48-h and 7-day mortality. CLs can be a reliable tool for their inexpensiveness, rapidity and reliability in identifying septic patients at high risk of short-term mortality.