bluuebunny/crossref_metadata_2025 huggingface dataset:
Religion’s Political Role in Rawlsian Key
Authors: van der Ven Johannes A.
Published: November 2012

Abstract

In Political Liberalism, expanded edition, Rawls repeatedly wants religions to accept liberal democracy for intrinsic reasons from their own religious premises, not as a modus vivendi. This article is to be considered an exploration in that field. In the first part the narrative of the St. Paul’s speech before the Areopagus in Athens by Luke is hermeneutically analyzed, as it tries to find common ground with Hellenistic philosophy and to do so by using deliberative rhetoric. In the second part these two characteristics of the Lukean story are considered the building blocks for the intrinsic acceptance of liberal democracy, albeit not in a substantive, but a formal key. The common ground Luke explored then was religious in nature, whereas in our days, at least in North-Western Europe, religion belongs to a cognitive minority. Moreover philosophy does not provide a common ground either, as there is a pluralism of competing schools nowadays. But intercontextual hermeneutics metaphorically permits to draw the following quadratic equation: as Lukean Paul related the Christian message to his philosophical context in order to find common ground, so we are to relate it to our context, the common ground of which is not philosophical, but political, which refers to the context of public reason. This article argues for accepting Rawls’ concept of using a bilingual language game for religion to present its religious convictions into the public debate and in due course translate them in terms of public reason. Such a translation requires a deliberative argumentation, that corresponds to the rules of logics and epistemology in practical reason.
Willing, Wanting, Waiting
Authors: Ferrero Luca
Published: November 2012

In his book Willing, Wanting, Waiting Holton defends a comprehensive view of the will.  His central claims are: (i) that we have a capacity of choice, independent of judgment  about what is best to do, (ii) that resistance to temptation requires a special kind of intentions, resolutions, and the exercise of an executive capacity, willpower, (iii) there is a distinction between weakness of will and akrasia. I argue that Holton is right about these claims, but I raise a few concerns: (a) I am unclear about the philosophical import of (i);  (b) I find that important details in the explanation of the working of willpower vis-à-vis temptation are missing and that there are inconsistencies in his account of addiction;  (c) I would have liked a more extensive discussion of other possible defects of will; (d) I am unclear about the scope of the will and the relation of willpower to other executive capac­ities. I conclude with a brief assessment of the contribution of psychological studies to the philosophical investigation of intentions and the will.
An Early Doctrinal Controversy in the Iranian School of Ismaʿili Thought and Its Implications
Authors: K. Poonawala Ismail
Published: November 2012

Abstract

The controversy stemmed from Nasafi’sKetāb al-Mahsul, wherein he introduced a pre-Fārābian version of Neo-Platonism into Ismaʿili cosmology, adapting it to Shiʿi-Ismaʿili doctrines. It provoked a sharp reaction among the Ismaʿili missionaries of Khorasan. Rāzi, an accomplished theologian and Nasafi’s contemporary, wrote hisKetāb al-Eslāhmending Nasafi’s errors. This led Sejestāni to defend the views of his teacher Nasafi and rebut Rāzi’s arguments in hisKetāb al-Nosra. Kermāni tried to reconcile the debate from a vantage point of post-Fārābian philosophy in hisKetāb al-Riāz. The controversy demonstrates that even a difference of opinion concerning major doctrinal issues was tolerated and resolved by scholarly debate.
Transition, Co-Optation and Permeation—Evolution of the Party’s Organizing Techniques in a Challenging Environment
Authors: Yuejin Jing
Published: November 2012

Abstract

In the last 30 years, China’s ruling party, with its Reform and Opening, has made achievements that have astonished the world. In that same time span, the Party has overcome a variety of foreseeable and non-foreseeable challenges. Meeting these challenges, even turning them into momentum for reform and resources for institutional innovation, is a matter of great importance for the existence and evolution of the current system. This article considers three challenges facing the Party from the angle of organization-building: the challenge of democratic elections, revolving around village self-government; the challenge of social fragmentation resulting from the market economy; and the challenge, intimately linked to the first two, of a growing civil society. Amidst our missteps, as we “cross the river by feeling the stones”, the ruling Party has adopted various policy responses to these challenges. This article focuses on three organizing techniques: transition, co-optation, and permeation. After briefly introducing these three techniques, the article then attempts a holistic analysis and explores a few related theoretical issues.
Society Managing Society: Innovation in the System of Management for Social Organizations
Authors: Xiuping Zhou , Qiushi Liu
Published: November 2012

Abstract

Categorized management is one of the main lines of thinking on the reform of the Chinese system of management for social organizations. However, there is a divergence of opinion amongst different researchers on the standards for categorization. On the basis of the findings of international research about supportive social organizations, the authors attempt to set forth a way of categorizing social organizations into two main groups: supportive and implementing organizations. They go on to demonstrate that the management of implementing organizations through supportive social organizations is a new kind of innovation within the self-governance of society and system of management for social organizations in China. Three methods of supportive social organization management and service for implementing organizations are explored, including industry leadership, bridging between sectors and self-regulation. Finally, a description is given of the challenges faced by China’s supportive social organizations themselves during their own development.
The Specter of Yalta: Asia Firsters and the Development of Conservative Internationalism
Authors: Mao Joyce
Published: November 2012

During the 1940s, conservative leaders in the United States turned to the emerging Cold War in Asia both to condemn the moral bankruptcy of liberal globalism and to establish their own brand of anti-Communist internationalism. “Asia Firsters” such as Senators William F. Knowland, John W. Bricker, and Robert A. Taft evoked the specter of Yalta and Roosevelt’s betrayal of Nationalist China as a signature issue which extended far beyond the question of who “lost” China. Yalta served as a touchstone for the right’s ideological and political development during the Cold War. Focusing on U.S.-People’s Republic-Taiwan relations during the early and mid-1950s, this article traces how initial criticism of the 1945 agreements quickly evolved into practical legislative proposals that addressed executive overreach, legislative oversight, collective international peacekeeping, opposition to Beijing’s admission to the United Nations, and constitutional principles vis-à-vis active global interventionism. Although Asia Firsters failed to substantively change China policy, their approach was an inspiration for the most enduring American political movement of the postwar period.
The Effect of Video Game Training on the Vision of Adults with Bilateral Deprivation Amblyopia
Authors: Jeon Seong Taek , Maurer Daphne , Lewis Terri L.
Published: November 2012

Amblyopia is a condition involving reduced acuity caused by abnormal visual input during a critical period beginning shortly after birth. Amblyopia is typically considered to be irreversible during adulthood. Here we provide the first demonstration that video game training can improve at least some aspects of the vision of adults with bilateral deprivation amblyopia caused by a history of bilateral congenital cataracts. Specifically, after 40 h of training over one month with an action video game, most patients showed improvement in one or both eyes on a wide variety of tasks including acuity, spatial contrast sensitivity, and sensitivity to global motion. As well, there was evidence of improvement in at least some patients for temporal contrast sensitivity, single letter acuity, crowding, and feature spacing in faces, but not for useful field of view. The results indicate that, long after the end of the critical period for damage, there is enough residual plasticity in the adult visual system to effect improvements, even in cases of deep amblyopia caused by early bilateral deprivation.
Contribution of Disparity to the Perception of 3D Shape as Revealed by Bistability of Stereoscopic Necker Cubes
Authors: Erkelens C. J.
Published: November 2012

The Necker cube is a famous demonstration of ambiguity in visual perception of 3D shape. Its bistability is attributed to indecisiveness because monocular cues do not allow the observer to infer one particular 3D shape from the 2D image. A remarkable but not appreciated observation is that Necker cubes are bistable during binocular viewing. One would expect disparity information to veto bistability. To investigate the effect of zero and non-zero disparity on perceptual bistability in detail, perceptual dominance durations were measured for luminance- and disparity-defined Necker cubes. Luminance-defined Necker cubes were bistable for all tested disparities between the front and back faces of the cubes. Absence of an effect of disparity on dominance durations suggested the suppression of disparity information. Judgments of depth between the front and back sides of the Necker cubes, however, showed that disparity affected perceived depth. Disparity-defined Necker cubes were also bistable but dominance durations showed different distributions. I propose a framework for 3D shape perception in which 3D shape is inferred from pictorial cues acting on luminance- and disparity-defined 2D shapes.
The Continuum of Violence against Women and the Challenges of Effective Redress
Authors: Manjoo Rashida
Published: November 2012

Globally violence against women is a systemic and widespread problem. Despite the recognition of such violence as a violation of human rights, its numerous manifestations and increasing prevalence rates are a source of concern. The mandate of the United Nations (UN) Special Rapporteur on violence against women, its causes and consequences has over the eighteen years of its existence examined the phenomena both conceptually and in practice, through thematic reports and country mission reports, respectively. This article focuses on 4 aspects of violence against women. (1) It provides an overview of the evolution of violence against women as a human rights issue. (2) It examines the different manifestations of violence against women. (3) It examines the interpretation by States of their obligation to exercise due diligence in responding to and preventing violence against women. (4) It proposes a holistic approach to dealing with the issue of Violence against Women.
Postoperative Facial Nerve Function in Pediatric Parotidectomy
Authors: Owusu James A. , Parker Noah P. , Rimell Frank L.
Published: November 2012

Objective

To analyze the incidence of postparotidectomy facial nerve dysfunction in pediatric patients and to evaluate the association between patient demographics or underlying pathology and functional outcomes.

Study Design

Case series with chart review.

Setting

Tertiary‐care pediatric hospital.

Subjects and Methods

We reviewed charts of patients younger than 18 years who underwent parotidectomy performed by a pediatric otolaryngologist between 1999 and 2011 at a tertiary pediatric center. Distributions of postoperative facial nerve dysfunction and timing of recovery were noted. Chi‐square and t‐test analyses were conducted to determine the association between demographics or pathology and functional outcomes.

Results

Forty‐three patients met inclusion criteria: 41 underwent superficial parotidectomy, and 2 underwent total parotidectomy. Leading indications for surgery were atypical mycobacterial infection (16/43 [37.2%]) and first branchial cleft anomaly (8/43 [18.6]). The facial nerve was sacrificed in 1 (2%) patient with Ewing’s sarcoma involving the nerve. The incidence of immediate facial nerve paresis was 21% (9/43), involving the marginal mandibular nerve (n = 7), buccal branch (n = 1), and both marginal mandibular and frontal branches (n = 1). Full recovery of nerve function in patients with paresis occurred within 1 month (n = 2), 2 months (n = 1), 6 months (n = 3), or 10 months (n = 2).

Conclusion

Postparotidectomy paresis of distal branches of the facial nerve is a common occurrence in the pediatric population and should be discussed during preoperative evaluation. The risk is comparable to that of the adult population. Age, gender, and pathologic diagnosis were not predictive of postoperative nerve dysfunction.
Antidepressant-like effect of oleanolic acid in mice exposed to the repeated forced swimming test
Authors: Yi Li-Tao , Li Jing , Liu Qing , Geng Di , Zhou Ya-Fei , Ke Xiao-Qing , Chen Huan , Weng Lian-Jin
Published: November 2012

The study aimed to explore the antidepressant-like effect of oleanolic acid and its possible mechanism related to the monoaminergic system and neurotrophin in mice exposed to the repeated forced swimming test (FST). Both the duration and the latency of immobility affected by oleanolic acid (10, 20 and 40 mg/kg) were evaluated in the FST repeated at intervals on days 1, 7 and 14, followed by neurochemical and brain-derived neurotrophic factor (BDNF) analyses in the mouse brain regions of frontal cortex and whole hippocampus. A repeated analysis of variance (ANOVA) indicated that over retesting the immobility time increased, whereas latency to immobility tended to decrease. Minute-by-minute analysis showed that immobility time also increased during the 4-min course of the test. In addition, post-hoc Dunnett’s test demonstrated that sub-chronic and chronic, but not acute, oleanolic acid treatment reduced the immobility time (sub-chronic: 20 mg/kg, 43.5%; chronic: 10 mg/kg, 19.3%; 20 mg/kg, 31.8%) and increased the latency to immobility (sub-chronic: 10 mg/kg, 60.6%; 20 mg/kg, 80.1%; chronic: 10 mg/kg, 121.8%; 20 mg/kg, 140.8%; 40 mg/kg, 80.0%). Furthermore, chronic administration of oleanolic acid significantly increased serotonin (5-HT) levels (frontal cortex: 44.5%, 41.9%, 27.5% for 10, 20, 40 mg/kg; hippocampus: 57.2%, 80.9% for 10, 20 mg/kg), decreased 5-hydroxyindoleacetic acid (5-HIAA)/5-HT ratio (frontal cortex: 31.6%, 30.1%, 23.5%; hippocampus: 40.6%, 47.7%, 29.2% for 10, 20, 40 mg/kg) and elevated norepinephrine (NE) levels (hippocampus: 20 mg/kg, 45.4%) but did not alter dopamine (DA) levels. Moreover, BDNF levels in the two brain regions were also elevated by chronic oleanolic acid treatment (frontal cortex: 20 mg/kg, 67.2%; hippocampus: 10 mg/kg, 36.4%; 20 mg/kg, 55.1%). Taken together, these findings imply that functions of 5-HT, NE and BDNF may be involved in the antidepressant-like effect of oleanolic acid.
Quality and quantity of oral participation and English proficiency gains
Authors: Delaney Thomas
Published: November 2012

There are many reasons to believe that oral participation in the target language (TL) is beneficial for classroom language learners. In addition to the prominence current second language acquisition (SLA) theory gives to processes that assume learner production of the TL (e.g. negotiation of meaning), teachers often view oral participation as a measure of learner involvement. Thus, it is often assumed that learners who participate often are likely to make greater proficiency gains. This correlational study examined this assumption by investigating the relationship between learners’ oral participation in classes for English as a foreign language (EFL) at a Japanese university and their gains in TL proficiency. Results indicated that while the quality of learners’ participation (accuracy, complexity, and fluency) was positively related to gains in TL ability, quantity of participation was not. While not indicative of a cause–effect relationship, the results suggest that teachers might wish to consider encouraging quality of participation as well as quantity.
Improving public perceptions around cleanliness and health care associated infection in hospitals (service improvement)
Authors: Whatley Vanessa , Jackson Louise , Taylor Jane
Published: November 2012

A qualitative study was undertaken to understand public perceptions of hospital cleanliness and how this might be improved following national initiatives to reduce infection rates and invest in domestic services. Eleven focus groups were held across the West Midlands.

Results demonstrated that perceptions are attained from a variety of sources. For patients and visitors the things they see, hear, smell and taste in hospitals and health services shaped their perceptions of cleanliness. For the public the media, friends and family are important. Key factors for attention include: ensuring hospital floors, walls, ceilings, doors and toilets are kept clean, tidy, and unstained; an absence of strong odours; patients have the opportunity for regular baths/showers; staff have clean hands and uniforms, have short, clean finger nails and hair tied back; and eye-catching material on why cleanliness is important as well as what people need to do. Participants were generally unaware of the symptoms of MRSA, Clostridium difficile or Norovirus.

The results of the study suggest that attention needs to be focussed on developing effective communication strategies. This will only be possible by adopting a multifaceted approach and enabling collaborative working between infection control staff, communications teams and other frontline staff.
Genome-level identification, gene expression, and comparative analysis of porcine β-defensin genes
Authors: Choi Min-Kyeung , Le Minh Thong , Nguyen Dinh Truong , Choi Hojun , Kim Won , Kim Jin-Hoi , Chun Jungwan , Hyeon Jiyeon , Seo Kunho , Park Chankyu
Published: November 2012

Background

Beta-defensins (β-defensins) are innate immune peptides with evolutionary conservation across a wide range of species and has been suggested to play important roles in innate immune reactions against pathogens. However, the complete β-defensin repertoire in the pig has not been fully addressed.

Result

A BLAST analysis was performed against the available pig genomic sequence in the NCBI database to identify β-defensin-related sequences using previously reported β-defensin sequences of pigs, humans, and cattle. The porcine β-defensin gene clusters were mapped to chromosomes 7, 14, 15 and 17. The gene expression analysis of 17 newly annotated porcine β-defensin genes across 15 tissues using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) showed differences in their tissue distribution, with the kidney and testis having the largest pBD expression repertoire. We also analyzed single nucleotide polymorphisms (SNPs) in the mature peptide region of pBD genes from 35 pigs of 7 breeds. We found 8 cSNPs in 7 pBDs.

Conclusion

We identified 29 porcine β-defensin (pBD) gene-like sequences, including 17 unreported pBDs in the porcine genome. Comparative analysis of β-defensin genes in the pig genome with those in human and cattle genomes showed structural conservation of β-defensin syntenic regions among these species.
A de novo germline mutation in MYH7 causes a progressive dominant myopathy in pigs
Authors: Murgiano Leonardo , Tammen Imke , Harlizius Barbara , Drögemüller Cord
Published: November 2012

Background

About 9% of the offspring of a clinically healthy Piétrain boar named ‘Campus’ showed a progressive postural tremor called Campus syndrome (CPS). Extensive backcross experiments suggested a dominant mode of inheritance, and the founder boar was believed to be a gonadal mosaic. A genome-scan mapped the disease-causing mutation to an 8 cM region of porcine chromosome 7 containing the MHY7 gene. Human distal myopathy type 1 (MPD1), a disease partially resembling CPS in pigs, has been associated with mutations in the MYH7 gene.

Results

The porcine MYH7 gene structure was predicted based on porcine reference genome sequence, porcine mRNA, and in comparison to the human ortholog. The gene structure was highly conserved with the exception of the first exon. Mutation analysis of a contiguous genomic interval of more than 22 kb spanning the complete MYH7 gene revealed an in-frame insertion within exon 30 of MYH7 (c.4320_4321insCCCGCC) which was perfectly associated with the disease phenotype and confirmed the dominant inheritance. The mutation is predicted to insert two amino acids (p.Ala1440_Ala1441insProAla) in a very highly conserved region of the myosin tail. The boar ‘Campus’ was shown to be a germline and somatic mosaic as assessed by the presence of the mutant allele in seven different organs.

Conclusion

This study illustrates the usefulness of recently established genomic resources in pigs. We have identified a spontaneous mutation in MYH7 as the causative mutation for CPS. This paper describes the first case of a disorder caused by a naturally occurring mutation in the MYH7 gene of a non-human mammalian species. Our study confirms the previous classification as a primary myopathy and provides a defined large animal model for human MPD1. We provide evidence that the CPS mutation occurred during the early development of the boar ‘Campus’. Therefore, this study provides an example of germline mosaicism with an asymptomatic founder.
Development of a genetic tool for product regulation in the diverse British pig breed market
Authors: Wilkinson Samantha , Archibald Alan L , Haley Chris S , Megens Hendrik-Jan , Crooijmans Richard PMA , Groenen Martien AM , Wiener Pamela , Ogden Rob
Published: November 2012

Background

The application of DNA markers for the identification of biological samples from both human and non-human species is widespread and includes use in food authentication. In the food industry the financial incentive to substituting the true name of a food product with a higher value alternative is driving food fraud. This applies to British pork products where products derived from traditional pig breeds are of premium value. The objective of this study was to develop a genetic assay for regulatory authentication of traditional pig breed-labelled products in the porcine food industry in the United Kingdom.

Results

The dataset comprised of a comprehensive coverage of breed types present in Britain: 460 individuals from 7 traditional breeds, 5 commercial purebreds, 1 imported European breed and 1 imported Asian breed were genotyped using the PorcineSNP60 beadchip. Following breed-informative SNP selection, assignment power was calculated for increasing SNP panel size. A 96-plex assay created using the most informative SNPs revealed remarkably high genetic differentiation between the British pig breeds, with an average FST of 0.54 and Bayesian clustering analysis also indicated that they were distinct homogenous populations. The posterior probability of assignment of any individual of a presumed origin actually originating from that breed given an alternative breed origin was > 99.5% in 174 out of 182 contrasts, at a test value of log(LR) > 0. Validation of the 96-plex assay using independent test samples of known origin was successful; a subsequent survey of market samples revealed a high level of breed label conformity.

Conclusion

The newly created 96-plex assay using selected markers from the PorcineSNP60 beadchip enables powerful assignment of samples to traditional breed origin and can effectively identify mislabelling, providing a highly effective tool for DNA analysis in food forensics.
The origin of modern frogs (Neobatrachia) was accompanied by acceleration in mitochondrial and nuclear substitution rates
Authors: Irisarri Iker , Mauro Diego San , Abascal Federico , Ohler Annemarie , Vences Miguel , Zardoya Rafael
Published: November 2012

Background

Understanding the causes underlying heterogeneity of molecular evolutionary rates among lineages is a long-standing and central question in evolutionary biology. Although several earlier studies showed that modern frogs (Neobatrachia) experienced an acceleration of mitochondrial gene substitution rates compared to non-neobatrachian relatives, no further characterization of this phenomenon was attempted. To gain new insights on this topic, we sequenced the complete mitochondrial genomes and nine nuclear loci of one pelobatoid (Pelodytes punctatus) and five neobatrachians, Heleophryne regis (Heleophrynidae), Lechriodus melanopyga (Limnodynastidae), Calyptocephalella gayi (Calyptocephalellidae), Telmatobius bolivianus (Ceratophryidae), and Sooglossus thomasseti (Sooglossidae). These represent major clades not included in previous mitogenomic analyses, and most of them are remarkably species-poor compared to other neobatrachians.

Results

We reconstructed a fully resolved and robust phylogeny of extant frogs based on the new mitochondrial and nuclear sequence data, and dated major cladogenetic events. The reconstructed tree recovered Heleophryne as sister group to all other neobatrachians, the Australasian Lechriodus and the South American Calyptocephalella formed a clade that was the sister group to Nobleobatrachia, and the Seychellois Sooglossus was recovered as the sister group of Ranoides. We used relative-rate tests and direct comparison of branch lengths from mitochondrial and nuclear-based trees to demonstrate that both mitochondrial and nuclear evolutionary rates are significantly higher in all neobatrachians compared to their non-neobatrachian relatives, and that such rate acceleration started at the origin of Neobatrachia.

Conclusions

Through the analysis of the selection coefficient (ω) in different branches of the tree, we found compelling evidence of relaxation of purifying selection in neobatrachians, which could (at least in part) explain the observed higher mitochondrial and nuclear substitution rates in this clade. Our analyses allowed us to discard that changes in substitution rates could be correlated with increased mitochondrial genome rearrangement or diversification rates observed in different lineages of neobatrachians.
PMRD: a curated database for genes and mutants involved in plant male reproduction
Authors: Cui Xiao , Wang Qiudao , Yin Wenzhe , Xu Huayong , Wilson Zoe A , Wei Chaochun , Pan Shenyuan , Zhang Dabing
Published: November 2012

Background

Male reproduction is an essential biological event in the plant life cycle separating the diploid sporophyte and haploid gametophyte generations, which involves expression of approximately 20,000 genes. The control of male reproduction is also of economic importance for plant breeding and hybrid seed production. With the advent of forward and reverse genetics and genomic technologies, a large number of male reproduction-related genes have been identified. Thus it is extremely challenging for individual researchers to systematically collect, and continually update, all the available information on genes and mutants related to plant male reproduction. The aim of this study is to manually curate such gene and mutant information and provide a web-accessible resource to facilitate the effective study of plant male reproduction.

Description

Plant Male Reproduction Database (PMRD) is a comprehensive resource for browsing and retrieving knowledge on genes and mutants related to plant male reproduction. It is based upon literature and biological databases and includes 506 male sterile genes and 484 mutants with defects of male reproduction from a variety of plant species. Based on Gene Ontology (GO) annotations and literature, information relating to a further 3697 male reproduction related genes were systematically collected and included, and using in text curation, gene expression and phenotypic information were captured from the literature. PMRD provides a web interface which allows users to easily access the curated annotations and genomic information, including full names, symbols, locations, sequences, expression patterns, functions of genes, mutant phenotypes, male sterile categories, and corresponding publications. PMRD also provides mini tools to search and browse expression patterns of genes in microarray datasets, run BLAST searches, convert gene ID and generate gene networks. In addition, a Mediawiki engine and a forum have been integrated within the database, allowing users to share their knowledge, make comments and discuss topics.

Conclusion

PMRD provides an integrated link between genetic studies and the rapidly growing genomic information. As such this database provides a global view of plant male reproduction and thus aids advances in this important area.
Molecular characterization of rotavirus isolates from select Canadian pediatric hospitals
Authors: McDermid Andrew , Le Saux Nicole , Grudeski Elsie , Bettinger Julie A , Manguiat Kathy , Halperin Scott A , MacDonald Lily , Déry Pierre , Embree Joanne , Vaudry Wendy , Booth Timothy F ,   Members of the Canadian Immunization Monitoring Program, Active (IMPACT)
Published: November 2012

Background

We report the first multi-site rotavirus genotype analysis in Canada. Prior to this study, there was a dearth of rotavirus G and P genotyping data in Canada. Publically funded universal rotavirus vaccination in Canada started in 2011 and has been introduced by four provinces to date. Uptake of rotavirus vaccines in Canada prior to 2012 has been very limited. The aim of this study was to describe the genotypes of rotavirus strains circulating in Canada prior to widespread implementation of rotavirus vaccine by genotyping samples collected from selected paediatric hospitals. Secondly we identified rotavirus strains that differed genetically from those included in the vaccines and which could affect vaccine effectiveness.

Methods

Stool specimens were collected by opportunity sampling of children with gastroenteritis who presented to emergency departments. Samples were genotyped for G (VP7) genotypes and P (VP4) genotypes by hemi-nested multiplex PCR methods. Phylogenetic analysis was carried out on Canadian G9 strains to investigate their relationship to G9 strains that have circulated in other regions of the world.

Results

348 samples were collected, of which 259 samples were rotavirus positive and genotyped. There were 34 rotavirus antigen immunoassay negative samples genotyped using PCR-based methods. Over the four rotavirus seasons, 174 samples were G1P[8], 45 were G3P[8], 22 were G2P[4], 13 were G9P[8], 3 were G4P[8] and 2 were G9P[4]. Sequence analysis showed that all Canadian G9 isolates are within lineage III.

Conclusions

Although a limited number of samples were obtained from a median of 4 centres during the 4 years of the study, it appears that currently approved rotavirus vaccines are well matched to the rotavirus genotypes identified at these hospitals. Further surveillance to monitor the emergence of rotavirus genotypes in Canada is warranted.
APOA5 Q97X Mutation Identified through homozygosity mapping causes severe hypertriglyceridemia in a Chilean consanguineous family
Authors: Dussaillant Catalina , Serrano Valentina , Maiz Alberto , Eyheramendy Susana , Cataldo Luis Rodrigo , Chavez Matías , Smalley Susan V , Fuentes Marcela , Rigotti Attilio , Rubio Lorena , Lagos Carlos F , Martinez José Alfredo , Santos José Luis
Published: November 2012

Background

Severe hypertriglyceridemia (HTG) has been linked to defects in LPL, APOC2, APOA5, LMF1 and GBIHBP1 genes. However, a number of severe HTG cases are probably caused by as yet unidentified mutations. Very high triglyceride plasma levels (>112 mmol/L at diagnosis) were found in two sisters of a Chilean consanguineous family, which is strongly suggestive of a recessive highly penetrant mutation. The aim of this study was to determine the genetic locus responsible for the severe HTG in this family.

Methods

We carried out a genome-wide linkage study with nearly 300,000 biallelic markers (Illumina Human CytoSNP-12 panel). Using the homozygosity mapping strategy, we searched for chromosome regions with excess of homozygous genotypes in the affected cases compared to non-affected relatives.

Results

A large homozygous segment was found in the long arm of chromosome 11, with more than 2,500 consecutive homozygous SNP shared by the proband with her affected sister, and containing the APOA5/A4/C3/A1 cluster. Direct sequencing of the APOA5 gene revealed a known homozygous nonsense Q97X mutation (p.Gln97Ter) found in both affected sisters but not in non-affected relatives nor in a sample of unrelated controls.

Conclusion

The Q97X mutation of the APOA5 gene in homozygous status is responsible for the severe hypertriglyceridemia in this family. We have shown that homozygosity mapping correctly pinpointed the genomic region containing the gene responsible for severe hypertriglyceridemia in this consanguineous Chilean family.
Percutaneous treatment of a aorto-caval fistula in a old high risk patient
Authors: Rapacciuolo Antonio , De Angelis Maria Carmen , di Pietro Elisa , Puglia Roberto , Di Tommaso Ettore , Ruggiero Danilo , Amato Bruno , Iannelli Gabriele
Published: November 2012

Background

To remark the feasibility of endovascular treatment of an aorto-caval fistula in a old high risk patient with “hostile” abdomen for previous surgeries.

Methods

In September 2009 a 81-years-old patient was admitted in emergency at our department because of abdominal pain and massive oedema of the lower extremities associated to dyspnoea (New York Heart Association (NYHA) functional class III). A CT scan showed an aorto-caval fistula involving the abdominal aorta below the renal arteries. This abnormal communication was likely due to the previous abdominal surgeries, was complicated by occlusion of the inferior vena cava at the diaphragm and was responsible for the massive oedema of the lower extremities. Because of unstable conditions and hostile abdomen the patient was considered unfit for conventional surgery and an endovascular approach was planned. After unsuccessful attempt by positioning of an Amplatzer vascular ring into the fistula, a Medtronic covered stent-grafts were implanted from the renal arteries to the both common iliac arteries. The patient had an impressive improvement characterized by a 18 Kg weight loss and a complete restoration of the functional capacity (from NYHA class III to NYHA class I) associated to a complete resolution of the lower extremities oedema as confirmed at the a month-CT-scan.

Conclusion

Endovascular surgery of aorto-caval fistula represents a good option in alternative to conventional surgery mostly in old high risk patient.
A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C
Authors: Kumar Rajesh , Andrabi Raiees , Tiwari Ashutosh , Prakash Somi Sankaran , Wig Naveet , Dutta Durgashree , Sankhyan Anurag , Khan Lubina , Sinha Subrata , Luthra Kalpana
Published: November 2012

Background

Production of human monoclonal antibodies that exhibit broadly neutralizing activity is needed for preventing HIV-1 infection, however only a few such antibodies have been generated till date. Isolation of antibodies by the hybridoma technology is a cumbersome process with fewer yields. Further, the loss of unstable or slowly growing clones which may have unique binding specificities often occurs during cloning and propagation and the strongly positive clones are often lost. This has been avoided by the process described in this paper, wherein, by combining the strategy of EBV transformation and recombinant DNA technology, we constructed human single chain variable fragments (scFvs) against the third variable region (V3) of the clade C HIV-1 envelope.

Results

An antigen specific phage library of 7000 clones was constructed from the enriched V3- positive antibody secreting EBV transformed cells. By ligation of the digested scFv DNA into phagemid vector and bio panning against the HIV-1 consensus C and B V3 peptides followed by random selection of 40 clones, we identified 15 clones that showed V3 reactivity in phage ELISA. DNA fingerprinting analysis and sequencing showed that 13 out of the 15 clones were distinct. Expression of the positive clones was tested by SDS-PAGE and Western blot. All the 13 anti-V3 scFvs showed cross-reactivity against both the clade C and B V3 peptides and did not show any reactivity against other unrelated peptides in ELISA. Preliminary neutralization assays indicated varying degrees of neutralization of clade C and B viruses. EBV transformation, followed by antigen selection of lines to identify specific binders, enabled the selection of phage from un-cloned lines for scFv generation, thus avoiding the problems of hybridoma technology. Moreover, as the clones were pretested for antigen binding, a comparatively small library sufficed for the selection of a considerable number of unique antigen binding phage. After selection, the phage clones were propagated in a clonal manner.

Conclusions

This strategy can be efficiently used and is cost effective for the generation of diverse recombinant antibodies. This is the first study to generate anti-V3 scFvs against HIV-1 Clade C.
Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase
Authors: Abrahim Noor Nazirahanie , Kanthimathi M S , Abdul-Aziz Azlina
Published: November 2012

Background

Breast cancer is the most common form of cancer and the focus on finding chemotherapeutic agents have recently shifted to natural products.Piper betleis a medicinal plant with various biological activities. However, not much data is available on the anti-cancer effects ofP. betleon breast cancer. Due to the current interest in the potential effects of antioxidants from natural products in breast cancer treatment, we investigated the antioxidant activities of the leaves ofP. betleand its inhibitory effect on the proliferation of the breast cancer cell line, MCF-7.

Methods

The leaves ofP. betlewere extracted with solvents of varying polarities (water, methanol, ethyl acetate and hexane) and their phenolic and flavonoid content were determined using colorimetric assays. Phenolic composition was characterized using HPLC. Antioxidant activities were measured using FRAP, DPPH, superoxide anion, nitric oxide and hyroxyl radical scavenging assays. Biological activities of the extracts were analysed using MTT assay and antioxidant enzyme (catalase, superoxide dismutase, glutathione peroxidase) assays in MCF-7 cells.

Results

Overall, the ethyl acetate extract showed the highest ferric reducing activity and radical scavenging activities against DPPH, superoxide anion and nitric oxide radicals. This extract also contained the highest phenolic content implying the potential contribution of phenolics towards the antioxidant activities. HPLC analyses revealed the presence of catechin, morin and quercetin in the leaves. The ethyl acetate extract also showed the highest inhibitory effect against the proliferation of MCF-7 cells (IC50=65 μg/ml). Treatment of MCF-7 cells with the plant extract increased activities of catalase and superoxide dismutase.

Conclusions

Ethyl acetate is the optimal solvent for the extraction of compounds with antioxidant and anti-proliferative activities. The increased activities of catalase and superoxide dismutase in the treated cells could alter the antioxidant defense system, potentially contributing towards the anti-proliferative effect. There is great potential for the ethyl acetate extract ofP. betleleaf as a source of natural antioxidants and to be developed as therapeutics in cancer treatment.
Efficacy and safety comparison between liraglutide as add-on therapy to insulin and insulin dose-increase in Chinese subjects with poorly controlled type 2 diabetes and abdominal obesity
Authors: Li Chun-jun , Li Jing , Zhang Qiu-mei , Lv Lin , Chen Rui , Lv Chun-feng , Yu Pei , Yu De-min
Published: November 2012

Objective

To assess the efficacy and safety of adding liraglutide to established insulin therapy in poorly controlled Chinese subjects with type 2 diabetes and abdominal obesity compared with increasing insulin dose.

Methods

A 12-week, randomized, parallel-group study was carried out. A total of 84 patients completed the trial who had been randomly assigned to either the liraglutide-added group or the insulin-increasing group while continuing current insulin based treatment. Insulin dose was reduced by 0-30% upon the initiation of liraglutide. Insulin doses were subsequently adjusted to optimized glycemic control. Glycosylated hemoglobin (HbA1c) values, blood glucose, total daily insulin dose, body weight, waist circumference, and the number of hypoglycemic events and adverse events were evaluated.

Results

At the end of study, the mean reduction in HbA1c between the liraglutide-added group and the insulin-increasing group was not significantly different (1.9% vs. 1.77%, p>0.05). However, the percentage of subjects reaching the composite endpoint of HbA1c ≤ 7.0% with no weight gain and no hypoglycemia, was significantly higher in the liraglutide-added group than in the insulin-increasing group (67% vs. 19%, p<0.001). Add-on liraglutide treatment significantly reduced mean body weight (5.62 kg, p<0.01), waist circumference (5.70 cm, p<0.01), body mass index (BMI) (1.93 kg/m2, p<0.01) and daily total insulin dose (dropped by 66%) during 12-week treatment period, while all of these significantly increased with insulin increasing treatment. Add-on liraglutide treated patients had lower rate of hypoglycemic events and greater insulin and oral antidiabetic drugs discontinuation. Gastrointestinal disorders were the most common adverse events in the liraglutide added treatment, but were transient.

Conclusions

Addition of liraglutide to abdominally obese, insulin-treated patients led to improvement in glycemic control similar to that achieved by increasing insulin dosage, but with a lower daily dose of insulin and fewer hypoglycemic events. Adding liraglutide to insulin also induced a significant reduction in body weight and waist circumference. Liraglutide combined with insulin may be the best treatment option for poorly controlled type 2 diabetes and abdominal obesity.
In silico biosynthesis of virenose, a methylated deoxy-sugar unique to Coxiella burnetii lipopolysaccharide
Authors: Flores-Ramirez Gabriela , Janecek Stefan , Miernyk Ján A , Skultety Ludovit
Published: November 2012

Background

Coxiella burnetii is Gram-negative bacterium responsible for the zoonosis Q-fever. While it has an obligate intracellular growth habit, it is able to persist for extended periods outside of a host cell and can resist environmental conditions that would be lethal to most prokaryotes. It is these extracellular bacteria that are the infectious stage encountered by eukaryotic hosts. The intracellular form has evolved to grow and replicate within acidified parasitophorous vacuoles. The outer coat of C. burnetii comprises a complex lipopolysaccharide (LPS) component that includes the unique methylated-6-deoxyhexose, virenose. Although potentially important as a biomarker for C. burnetii, the pathway for its biosynthesis remains obscure.

Results

The 6-deoxyhexoses constitute a large family integral to the LPS of many eubacteria. It is believed that precursors of the methylated-deoxyhexoses traverse common early biosynthetic steps as nucleotide-monosaccharides. As a prelude to a full biosynthetic characterization, we present herein the results from bioinformatics-based, proteomics-supported predictions of the pathway for virenose synthesis. Alternative possibilities are considered which include both GDP-mannose and TDP-glucose as precursors.

Conclusion

We propose that biosynthesis of the unique C. burnetii biomarker, virenose, involves an early pathway similar to that of other C-3’-methylated deoxysugars which then diverges depending upon the nucleotide-carrier involved. The alternatives yield either the D- or L-enantiomers of virenose. Both pathways require five enzymatic steps, beginning with either glucose-6-phosphate or mannose-6-phosphate. Our in silico results comprise a model for virenose biosynthesis that can be directly tested. Definition of this pathway should facilitate the development of therapeutic agents useful for treatment of Q fever, as well as allowing improvements in the methods for diagnosing this highly infectious disease.
Role of TLR4/NADPH oxidase/ROS-activated p38 MAPK in VCAM-1 expression induced by lipopolysaccharide in human renal mesangial cells
Authors: Lee I-Ta , Shih Ruey-Horng , Lin Chih-Chung , Chen Jung-Tsan , Yang Chuen-Mao
Published: November 2012

Background

In bacteria-induced glomerulonephritis, Toll-like receptor 4 (TLR4) activation by lipopolysaccharide (LPS, a key component of the outer membranes of Gram-negative bacteria) can increase oxidative stress and the expression of vascular cell adhesion molecule-1 (VCAM-1), which recruits leukocytes to the glomerular mesangium. However, the mechanisms underlying VCAM-1 expression induced by LPS are still unclear in human renal mesangial cells (HRMCs).

Results

We demonstrated that LPS induced VCAM-1 mRNA and protein levels associated with an increase in the promoter activity of VCAM-1, determined by Western blot, RT-PCR, and promoter assay. LPS-induced responses were inhibited by transfection with siRNAs of TLR4, myeloid differentiation factor 88 (MyD88), Nox2, Nox4, p47phox, c-Src, p38 MAPK, activating transcription factor 2 (ATF2), and p300 or pretreatment with the inhibitors of reactive oxygen species (ROS, edaravone), NADPH oxidase [apocynin (APO) or diphenyleneiodonium chloride (DPI)], c-Src (PP1), p38 MAPK (SB202190), and p300 (GR343). LPS induced NADPH oxidase activation, ROS production, and p47phox translocation from the cytosol to the membrane, which were reduced by PP1 or c-Src siRNA. We observed that LPS induced TLR4, MyD88, c-Src, and p47phox complex formation determined by co-immunoprecipitation and Western blot. We further demonstrated that LPS stimulated ATF2 and p300 phosphorylation and complex formation via a c-Src/NADPH oxidase/ROS/p38 MAPK pathway. Up-regulation of VCAM-1 led to enhancing monocyte adhesion to HRMCs challenged with LPS, which was inhibited by siRNAs of c-Src, p47phox, p38 MAPK, ATF2, and p300 or pretreatment with an anti-VCAM-1 neutralizing antibody.

Conclusions

In HRMCs, LPS-induced VCAM-1 expression was, at least in part, mediated through a TLR4/MyD88/ c-Src/NADPH oxidase/ROS/p38 MAPK-dependent p300 and ATF2 pathway associated with recruitment of monocyte adhesion to kidney. Blockade of these pathways may reduce monocyte adhesion via VCAM-1 suppression and attenuation of the inflammatory responses in renal diseases.
Condom use at last sexual relationship among adolescents of Santiago Island, Cape Verde, - West Africa
Authors: Tavares Carlos Mendes , Schor Néia , Valenti Vitor E , Kanikadan Paula Yuri Sugishita , de Abreu Luiz Carlos
Published: November 2012

Objective

To estimate factors associated with condom use at last sexual intercourse among adolescents.

Methods

Cross-sectional study of a representative sample of 368 sexually active adolescents aged 13–17 years from eight public high schools on Santiago Island, Cape Verde, 2007. The level of significance was 5.0% obtained from logistic regression, considering the association between condom use and socio-demographic, sexual and reproductive variables.

Results

The prevalence of condom use at last sexual intercourse was 94.9%. Factors associated with condom use at last sexual relationship were: non-Catholic religion (OR=0.68, 95%CI: 0.52; 0.88) and affective-sexual partnership before the interview (OR=5.15, 95%CI: 1.79; 14.80).

Conclusions

There was a high prevalence of condom use at last sexual intercourse of adolescents.