bluuebunny/crossref_metadata_2025 huggingface dataset:
Measuring and Understanding Food Insecurity in Australia: A Systematic Review
Authors: McKay Fiona H. , Haines Bronte C. , Dunn Matthew
Published: February 2019

The number of Australians seeking food aid has increased in recent years; however, the current variability in the measurement of food insecurity means that the prevalence and severity of food insecurity in Australia is likely underreported. This is compounded by infrequent national health surveys that measure food insecurity, resulting in outdated population-level food insecurity data. This review sought to investigate the breadth of food insecurity research conducted in Australia to evaluate how this construct is being measured. A systematic review was conducted to collate the available Australian research. Fifty-seven publications were reviewed. Twenty-two used a single-item measure to examine food security status; 11 used the United States Department of Agriculture (USDA) Household Food Security Survey Module (HFSSM); two used the Radimer/Cornell instrument; one used the Household Food and Nutrition Security Survey (HFNSS); while the remainder used a less rigorous or unidentified method. A wide range in prevalence and severity of food insecurity in the community was reported; food insecurity ranged from 2% to 90%, depending on the measurement tool and population under investigation. Based on the findings of this review, the authors suggest that there needs to be greater consistency in measuring food insecurity, and that work is needed to create a measure of food insecurity tailored for the Australian context. Such a tool will allow researchers to gain a clear understanding of the prevalence of food insecurity in Australia to create better policy and practice responses.
Family Functioning, Emotional Intelligence, and Values: Analysis of the Relationship with Aggressive Behavior in Adolescents
Authors: Pérez-Fuentes María del Carmen , Molero Jurado María del Mar , Barragán Martín Ana Belén , Gázquez Linares José Jesús
Published: February 2019

Aggressive behavior in adolescence is influenced by a diversity of individual, family, and social variables. The purpose of this study was to analyze the relationship between family functioning, emotional intelligence, and personal values for development with different types of aggression, as well as to establish profiles with these variables according to the aggression. The study was carried out with a sample of 317 high school students aged 13 to 18 years old. The study showed that stress management (emotional intelligence), positive adolescent development, and family functioning predominated in nonaggressive subjects with higher scores than aggressors did. There was also a negative relationship between the different types of aggression and emotional intelligence, positive values, and family functioning. In addition, two different profiles were found. The first profile had less family functioning, interpersonal emotional intelligence, stress management, and fewer personal and social values than the second profile.
Regulation of Nitric Oxide Production in the Developmental Programming of Hypertension and Kidney Disease
Authors: Hsu Chien-Ning , Tain You-Lin
Published: February 2019

Development of the kidney can be altered in response to adverse environments leading to renal programming and increased vulnerability to the development of hypertension and kidney disease in adulthood. By contrast, reprogramming is a strategy shifting therapeutic intervention from adulthood to early life to reverse the programming processes. Nitric oxide (NO) is a key mediator of renal physiology and blood pressure regulation. NO deficiency is a common mechanism underlying renal programming, while early-life NO-targeting interventions may serve as reprogramming strategies to prevent the development of hypertension and kidney disease. This review will first summarize the regulation of NO in the kidney. We also address human and animal data supporting the link between NO system and developmental programming of hypertension and kidney disease. This will be followed by the links between NO deficiency and the common mechanisms of renal programming, including the oxidative stress, renin–angiotensin system, nutrient-sensing signals, and sex differences. Recent data from animal studies have suggested that interventions targeting the NO pathway could be reprogramming strategies to prevent the development of hypertension and kidney disease. Further clinical studies are required to bridge the gap between animal models and clinical trials in order to develop ideal NO-targeting reprogramming strategies and to be able to have a lifelong impact, with profound savings in the global burden of hypertension and kidney disease.
The Membrane Proximal Domain of TRPV1 and TRPV2 Channels Mediates Protein–Protein Interactions and Lipid Binding In Vitro
Authors: Doñate-Macián Pau , Álvarez-Marimon Elena , Sepulcre Francesc , Vázquez-Ibar José Luis , Perálvarez-Marín Alex
Published: February 2019

Constitutive or regulated membrane protein trafficking is a key cell biology process. Transient receptor potential channels are somatosensory proteins in charge of detecting several physical and chemical stimuli, thus requiring fine vesicular trafficking. The membrane proximal or pre-S1 domain (MPD) is a highly conserved domain in transient receptor potential channels from the vanilloid (TRPV) subfamily. MPD shows traits corresponding to protein-protein and lipid-protein interactions, and protein regulatory regions. We have expressed MPD of TRPV1 and TRPV2 as green fluorescente protein (GFP)-fusion proteins to perform an in vitro biochemical and biophysical characterization. Pull-down experiments indicate that MPD recognizes and binds Soluble N-ethylmaleimide-sensitive factor Attachment Protein Receptors (SNARE). Synchrotron radiation scattering experiments show that this domain does not self-oligomerize. MPD interacts with phosphatidic acid (PA), a metabolite of the phospholipase D (PLD) pathway, in a specific manner as shown by lipid strips and Trp fluorescence quenching experiments. We show for the first time, to the best of our knowledge, the binding to PA of an N-terminus domain in TRPV channels. The presence of a PA binding domain in TRPV channels argues for putative PLD regulation. Findings in this study open new perspectives to understand the regulated and constitutive trafficking of TRPV channels exerted by protein-protein and lipid-protein interactions.
Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires
Authors: Terzieva Antonia , Dimitrova Violeta , Djerov Lyubomir , Dimitrova Petya , Zapryanova Silvina , Hristova Iana , Vangelov Ivaylo , Dimova Tanya
Published: February 2019

Pregnancy is a state where high and stage-dependent plasticity of the maternal immune system is necessary in order to equilibrate between immunosuppression of harmful responses towards the fetus and ability to fight infections. TCR γδ cells have been implicated in the responses in infectious diseases, in the regulation of immune responses, and in tissue homeostasis and repair. The variety of functions makes γδ T cells a particularly interesting population during pregnancy. In this study, we investigated the proportion, phenotype and TCR γ and δ repertoires of γδ T cells at the maternal–fetal interface and in the blood of pregnant women using FACS, immunohistochemistry and spectratyping. We found an enrichment of activated and terminally differentiated pro-inflammatory γδ T-cell effectors with specific location in the human decidua during early pregnancy, while no significant changes in their counterparts in the blood of pregnant women were observed. Our spectratyping data revealed polyclonal CDR3 repertoires of the δ1, δ2 and δ3 chains and γ2, γ3, γ4 and γ5 chains and oligoclonal and highly restricted CDR3γ9 repertoire of γδ T cells in the decidua and blood of pregnant women. Early pregnancy induces recruitment of differentiated pro-inflammatory γδ T-cell effectors with diverse TCR repertoires at the maternal–fetal interface.
An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Authors: Nagai-Okatani Chiaki , Nagai Misugi , Sato Takashi , Kuno Atsushi
Published: February 2019

Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method for cell type-selective glycomic profiling of tissue fragments collected by laser microdissection (LMD) under fluorescent histochemical visualization. We optimized each step of histochemical staining and confirmed the reliability and validity of glycomic profiling. Using the optimized procedure, glycomic profiles were obtained with 0.5 mm2 of stained thymic sections (5-μm-thick) from 8-week-old C57BL/6J male mice. The glycomic profiles of Ulex europaeus agglutinin-I (UEA-I)-stained medullary regions showed higher UEA-I signals than those of the morphologically determined medulla regions, indicating the utility of this method for UEA-I(+) cell-selective analysis. To further evaluate this method, tissue fragments was serially collected from stained and unstained areas of medullary epithelial cell probes (UEA-I and anti-cytokeratin 5 antibody) and a cortex-staining probe (peanut agglutinin). The medullary regions assigned by the three probes showed significantly different glycomic profiles, highlighting the difference in subpopulation recognition among the three probes, which was consistent with previous reports. In conclusion, our fluorescence LMD-LMA method enabled cell type-selective tissue glycomic analysis of pathological specimens and animal models, especially for glyco-biomarker discovery.
Podoplanin in Inflammation and Cancer
Authors: Quintanilla Miguel , Montero-Montero Lucía , Renart Jaime , Martín-Villar Ester
Published: February 2019

Podoplanin is a small cell-surface mucin-like glycoprotein that plays a crucial role in the development of the alveoli, heart, and lymphatic vascular system. Emerging evidence indicates that it is also involved in the control of mammary stem-cell activity and biogenesis of platelets in the bone marrow, and exerts an important function in the immune response. Podoplanin expression is upregulated in different cell types, including fibroblasts, macrophages, T helper cells, and epithelial cells, during inflammation and cancer, where it plays important roles. Podoplanin is implicated in chronic inflammatory diseases, such as psoriasis, multiple sclerosis, and rheumatoid arthritis, promotes inflammation-driven and cancer-associated thrombosis, and stimulates cancer cell invasion and metastasis through a variety of strategies. To accomplish its biological functions, podoplanin must interact with other proteins located in the same cell or in neighbor cells. The binding of podoplanin to its ligands leads to modulation of signaling pathways that regulate proliferation, contractility, migration, epithelial–mesenchymal transition, and remodeling of the extracellular matrix. In this review, we describe the diverse roles of podoplanin in inflammation and cancer, depict the protein ligands of podoplanin identified so far, and discuss the mechanistic basis for the involvement of podoplanin in all these processes.
Influence of a 30-Day Slow-Paced Breathing Intervention Compared to Social Media Use on Subjective Sleep Quality and Cardiac Vagal Activity
Authors: Laborde Sylvain , Hosang Thomas , Mosley Emma , Dosseville Fabrice
Published: February 2019

Breathing techniques are part of traditional relaxation methods; however, their influence on psychophysiological variables related to sleep is still unclear. Consequently, the aim of this paper was to investigate the influence of a 30-day slow-paced breathing intervention compared to social media use on subjective sleep quality and cardiac vagal activity (CVA, operationalized via high-frequency heart rate variability). Healthy participants (n = 64, 33 male, 31 female, M = 22.11, SD = 3.12) were randomly allocated to an experimental or control group. In the experimental group, they had to perform slow-paced breathing for 15 min each evening across a 30-day period. This was administered through a smartphone application. The control group used social media (e.g., Facebook, Instagram, Whatsapp) for the same duration. The night before and after the intervention, their CVA was assessed via a light portable Electrocardiogram (ECG) device, and they had to fill out the Pittsburgh Sleep Quality Index questionnaire. Results showed that in comparison to the use of social media, the slow-paced breathing technique improved subjective sleep quality and increased overnight CVA, while a tendency was observed for morning awakening CVA. Slow-paced breathing appears a promising cost-effective technique to improve subjective sleep quality and cardiovascular function during sleep in young healthy individuals.
Comparison of Rigid and Soft-Brace Treatments for Acute Osteoporotic Vertebral Compression Fracture: A Prospective, Randomized, Multicenter Study
Authors: Kato Tsuyoshi , Inose Hiroyuki , Ichimura Shoichi , Tokuhashi Yasuaki , Nakamura Hiroaki , Hoshino Masatoshi , Togawa Daisuke , Hirano Toru , Haro Hirotaka , Ohba Tetsuro , Tsuji Takashi , Sato Kimiaki , Sasao Yutaka , Takahata Masahiko , Otani Koji , Momoshima Suketaka , Tateishi Ukihide , Tomita Makoto , Takemasa Ryuichi , Yuasa Masato , Hirai Takashi , Yoshii Toshitaka , Okawa Atsushi
Published: February 2019

While bracing is the standard conservative treatment for acute osteoporotic compression fracture, the efficacy of different brace treatments has not been extensively studied. We aimed to clarify and compare the preventive effect of the different brace treatments on the deformity of the vertebral body and other clinical results in this patient cohort. This multicenter nationwide prospective randomized study included female patients aged 65–85 years with acute one-level osteoporotic compression fractures. We assigned patients within four weeks of injury to either a rigid-brace treatment or a soft-brace treatment. The main outcome measure was the anterior vertebral body compression percentage at 48 weeks. Secondary outcome measures included scores on the European Quality of Life-5 Dimensions (EQ-5D), visual analog scale (VAS) for lower back pain, and the Japanese Orthopaedic Association Back Pain Evaluation Questionnaire (JOABPEQ). A total of 141 patients were assigned to the rigid-brace group, whereas 143 patients were assigned to the soft-brace group. There were no statistically significant differences in the primary outcome and secondary outcome measures between groups. In conclusion, among patients with fresh vertebral compression fractures, the 12-week rigid-brace treatment did not result in a statistically greater prevention of spinal deformity, better quality of life, or lesser back pain than soft-brace.
Chimeric Antigen Receptor (CAR) T Cell Therapy in Acute Myeloid Leukemia (AML)
Authors: Hofmann Susanne , Schubert Maria-Luisa , Wang Lei , He Bailin , Neuber Brigitte , Dreger Peter , Müller-Tidow Carsten , Schmitt Michael
Published: February 2019

Despite high response rates after initial chemotherapy in patients with acute myeloid leukemia (AML), relapses occur frequently, resulting in a five-year-survival by <30% of the patients. Hitherto, allogeneic hemotopoietic stem cell transplantation (allo-HSCT) is the best curative treatment option in intermediate and high risk AML. It is the proof-of-concept for T cell-based immunotherapies in AML based on the graft-versus-leukemia (GvL)-effect, but it also bears the risk of graft-versus-host disease. CD19-targeting therapies employing chimeric antigen receptor (CAR) T cells are a breakthrough in cancer therapy. A similar approach for myeloid malignancies is highly desirable. This article gives an overview on the state-of-the art of preclinical and clinical studies on suitable target antigens for CAR T cell therapy in AML patients.
Photothermal-Triggered Shape Memory Polymer Prepared by Cross-Linking Porphyrin-Loaded Micellar Particles
Authors: Qian Wangqiu , Song Yufang , Shi Dongjian , Dong Weifu , Wang Xiaorong , Zhang Hongji
Published: February 2019

In this work, we fabricated porphyrin-loaded shape memory polymer (SMP) film by cross-linking micellar particles prepared by co-assembly of porphyrin compounds and amphiphilic macromolecules formulated by copolymerization of 2-butoxy ethanol (BCS), methyl methacrylate (MMA), butyl acrylate (BA) acrylic acid (AA), and diacetone acrylamide (DAAM). The experimental results revealed that this film was able to respond to the red light in terms of photothermal effect enabled by the porphyrin filler. The photothermal-triggered shape memory behaviors of the film were further examined in detail. It was noteworthy that this material was expected to have potential applications in the biomedical field due to the excellent biocompatibility of the porphyrin filler and the red-light source, which was optimal and safe enough for biomedical treatment.
An Elastodiffusive Orthotropic Euler–Bernoulli Beam Considering Diffusion Flux Relaxation
Authors: Tarlakovskii Dmitry , Zemskov Andrei
Published: February 2019

This article considers an unsteady elastic diffusion model of Euler–Bernoulli beam oscillations in the presence of diffusion flux relaxation. We used the model of coupled elastic diffusion for a homogeneous orthotropic multicomponent continuum to formulate the problem. A model of unsteady bending for the elastic diffusive Euler–Bernoulli beam was obtained using Hamilton’s variational principle. The Laplace transform on time and the Fourier series expansion by the spatial coordinate were used to solve the obtained problem.
Guidelines-Driven Educational Intervention Promotes Healthy Lifestyle Among Adolescents and Adults: A Serbian National Longitudinal Study
Authors: Đorđić Višnja , Božić Predrag , Milanović Ivana , Radisavljević Snežana , Batez Maja , Jorga Jagoda , Ostojić Sergej M.
Published: February 2019

Background and objectives: The effectiveness of short-term focused educational programs to change health behaviors across large populations seems to be poorly described so far. The main aim of the present study was to evaluate an age-specific 45-min educational program, designed in accordance with the current U.S. Department of Health and Human Services and U.S. Department of Agriculture dietary guidelines and physical activity (PA) guidelines, among adolescents and adults. Materials and Methods: We evaluated the health-promoting lifestyle habits by the Health-Promoting Lifestyle Profile (HPLP-II) at baseline and following 6–8 weeks post-education in a nationally representative sample of Serbian adolescents and adults (n = 3822). Results: The percentage of adolescents eating 3–5 servings of vegetables per day increased at follow-up (20.1% versus 23.1%, p = 0.001), with significantly more adolescents regularly reading food labels (from 12.2% at baseline to 14.2% at follow-up; p = 0.02). Taken together, mean HPLP-II scores in adolescents significantly improved for both diet (0.05 points; p < 0.0001) and PA (0.09 points; p < 0.0001), and for PA in adults (0.08 points; p < 0.0001). Hierarchical multiple regression analysis revealed that our model as a whole (including time of testing as a predictor variable, and age and gender as control variables) explained 3.0% of the variance in mean HPLP-II scores for diet (p = 0.942) and 3.0% for PA (p = 0.285) in adolescents, and 1.1% of the variance in HPLP-II scores for diet (p = 0.781) and 1.9% for PA (p = 0.075) in adults, respectively. Conclusions: It appears that a brief focused education can positively tackle unhealthy lifestyles in promoting good health in general population. Different modes of interactive communication used here appeared to strengthen participants’ capacities for lifestyle changes.
Fast Amide Bond Cleavage Assisted by a Secondary Amino and a Carboxyl Group—A Model for yet Unknown Peptidases?
Authors: V. Komarov Igor , Yu. Ishchenko Aleksandr , Hovtvianitsa Aleksandr , Stepanenko Viacheslav , Kharchenko Serhii , D. Bond Andrew , J. Kirby Anthony
Published: February 2019

Unconstrained amides that undergo fast hydrolysis under mild conditions are valuable sources of information about how amide bonds may be activated in enzymatic transformations. We report a compound possessing an unconstrained amide bond surrounded by an amino and a carboxyl group, each mounted in close proximity on a bicyclic scaffold. Fast amide hydrolysis of this model compound was found to depend on the presence of both the amino and carboxyl functions, and to involve a proton transfer in the rate-limiting step. Possible mechanisms for the hydrolytic cleavage and their relevance to peptide bond cleavage catalyzed by natural enzymes are discussed. Experimental observations suggest that the most probable mechanisms of the model compound hydrolysis might include a twisted amide intermediate and a rate-determining proton transfer.
Retro-1-Oligonucleotide Conjugates. Synthesis and Biological Evaluation
Authors: Agramunt Jordi , Pedroso Enrique , Kreda Silvia M. , Juliano Rudolph L. , Grandas Anna
Published: February 2019

Addition of small molecule Retro-1 has been described to enhance antisense and splice switching oligonucleotides. With the aim of assessing the effect of covalently linking Retro-1 to the biologically active oligonucleotide, three different derivatives of Retro-1 were prepared that incorporated a phosphoramidite group, a thiol or a 1,3-diene, respectively. Retro-1–oligonucleotide conjugates were assembled both on-resin (coupling of the phosphoramidite) and from reactions in solution (Michael-type thiol-maleimide reaction and Diels-Alder cycloaddition). Splice switching assays with the resulting conjugates showed that they were active but that they provided little advantage over the unconjugated oligonucleotide in the well-known HeLa Luc705 reporter system.
Promise of G-Quadruplex Structure Binding Ligands as Epigenetic Modifiers with Anti-Cancer Effects
Authors: Sengupta Antara , Ganguly Akansha , Chowdhury Shantanu
Published: February 2019

Evidences from more than three decades of work support the function of non-duplex DNA structures called G-quadruplex (G4) in important processes like transcription and replication. In addition, G4 structures have been studied in connection with DNA base modifications and chromatin/nucleosome arrangements. Recent work, interestingly, shows promise of G4 structures, through interaction with G4 structure-interacting proteins, in epigenetics—in both DNA and histone modification. Epigenetic changes are found to be intricately associated with initiation as well as progression of cancer. Multiple oncogenes have been reported to harbor the G4 structure at regulatory regions. In this context, G4 structure-binding ligands attain significance as molecules with potential to modify the epigenetic state of chromatin. Here, using examples from recent studies we discuss the emerging role of G4 structures in epigenetic modifications and, therefore, the promise of G4 structure-binding ligands in epigenetic therapy.
Thermo-Responsive Graphene Oxide/Poly(Ethyl Ethylene Phosphate) Nanocomposite via Ring Opening Polymerization
Authors: Jiang Xue , Lu Guolin , Huang Xiaoyu , Li Yu , Cao Fangqi , Chen Hong , Liu Wenbin
Published: February 2019

An efficient strategy for growing thermo-sensitive polymers from the surface of exfoliated graphene oxide (GO) is reported in this article. GO sheets with hydroxyls and epoxy groups on the surface were first prepared by modified Hummer’s method. Epoxy groups on GO sheets can be easily modified through ring-opening reactions, involving nucleophilic attack by tris(hydroxymethyl) aminomethane (TRIS). The resulting GO-TRIS sheets became a more versatile precursor for next ring opening polymerization (ROP) of ethyl ethylene phosphate (EEP), leading to GO-TRIS/poly(ethyl ethylene phosphate) (GO-TRIS-PEEP) nanocomposite. The nanocomposite was characterized by 1H NMR, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis (TGA), differential thermal gravity (DTG), transmission electron microscopy (TEM) and atomic force microscopy (AFM). Since hydrophilic PEEP chains make the composite separate into single layers through hydrogen bonding interaction, the dispersity of the functionalized GO sheets in water is significantly improved. Meanwhile, the aqueous dispersion of GO-TRIS-PEEP nanocomposite shows reversible temperature switching self-assembly and disassembly behavior. Such a smart graphene oxide-based hybrid material is promising for applications in the biomedical field.
How Low-Income Mothers Select and Adapt Recipes and Implications for Promoting Healthy Recipes Online
Authors: Tobey Lauren N. , Mouzong Christine , Angulo Joyce Senior , Bowman Sally , Manore Melinda M.
Published: February 2019

We describe a 5-year (2011–2015) qualitative evaluation to refine the content/delivery of the Food Hero social marketing campaign recipes to low-income mothers. Objectives were to: (1) identify characteristics looked for in recipes; (2) determine recipe sources; (3) understand motivation for seeking new recipes and recipe adaptations; and (4) identify recipe website characteristics users valued. Nine focus groups (n = 55) were conducted in Portland, Oregon. Participants (35–52 years) were primary caregivers for ≥ one child, the primary household food shoppers/preparers, enrolled in the Supplemental Nutrition Assistance Program (SNAP) and able to speak/read English. Participants reported having “go-to” family recipes and regularly searching online for new recipes, especially those using ingredients available/preferred by family members. Recipe websites with highest appeal were polished and engaging to mothers/children, offered user-ratings/comments and were reachable from search engines. Results identified key recommendations: (1) understand the target audience; (2) aim to add healthy/customizable recipes to family “go-to’ recipe rotations and understand the impact of generational influences (e.g. how mothers/grandmothers cooked) on family meals; and (3) create websites that meet target audience criteria. Seeking the target audience’s input about the content/delivery of recipes is an important formative step for obesity-prevention projects that include healthy recipes.
A Polyphenol-Enriched Supplement Exerts Potent Epigenetic-Protective Activity in a Cell-Based Model of Brain Ischemia
Authors: Faggi Lara , Porrini Vanessa , Lanzillotta Annamaria , Benarese Marina , Mota Mariana , Tsoukalas Dimitris , Parrella Edoardo , Pizzi Marina
Published: February 2019

Bioactive components, due in part to their epigenetic properties, are beneficial for preventing several human diseases including cerebrovascular pathologies. However, no clear demonstration supports the idea that these molecules still conserve their epigenetic effects when acting at very low concentrations reproducing the brain levels achieved after oral administration of a micronutrient supplement. In the present study, we used a cellular model of brain ischemia to investigate the neuroprotective and epigenetic activities of a commercially available micronutrient mixture (polyphenol-enriched micronutrient mixture, PMM) enriched in polyphenols ((-)-epigallocatechin-3-gallate, quercetin, resveratrol), α-lipoic acid, vitamins, amino acids and other micronutrients. Mimicking the suggested dietary supplementation, primary cultures of mouse cortical neurons were pre-treated with PMM and then subjected to oxygen glucose deprivation (OGD). Pre-treatment with PMM amounts to provide bioactive components in the medium in the nanomolar range potently prevented neuronal cell death. The protection was associated with the deacetylation of the lysin 310 (K310) on NF-κB/RelA as well as the deacetylation of H3 histones at the promoter of Bim, a pro-apoptotic target of ac-RelA(K310) in brain ischemia. Epigenetic regulators known to shape the acetylation state of ac-RelA(K310) moiety are the histone acetyl transferase CBP/p300 and the class III histone deacetylase sirtuin-1. In view of that evidence, the protection we here report unveils the efficacy of bioactive components endowed with either inhibitory activity on CBP/p300 or stimulating activity on the AMP-activated protein kinase–sirtuin 1 pathway. Our results support a potential synergistic effect of micronutrients in the PMM, suggesting that the intake of a polyphenol-based micronutrient mixture can reduce neuronal vulnerability to stressful conditions at concentrations compatible with the predicted brain levels reached by a single constituent after an oral dose of PMM.
Impact of SchisandraChinensis Bee Pollen on Nonalcoholic Fatty Liver Disease and Gut Microbiota in HighFat Diet Induced Obese Mice
Authors: Cheng Ni , Chen Sinan , Liu Xinyan , Zhao Haoan , Cao Wei
Published: February 2019

Schisandrachinensisbee pollen has been used as a health food in China for centuries; however, its bioactive constituents and functions are not very clear. In this study, we investigated the phenolic compounds of Schisandrachinensisbee pollen extract (SCPE) by UHPLC-Q-Orbitrap-HRMS/HPLC-DAD-ECD and its prevention from nonalcoholic fatty liver disease (NAFLD) and modulation of gut microbiota in high fat diet induced obese C57BL/6 mice. The results showed that 12 phenolic compounds were identified in SCPE, and naringenin, rutin and chrysin were the main constituents. The content of naringenin reached 1.89 mg/g, and total phenolic content (TPC) of SCPE were 101.83 mg GA/g. After obese mice were administrated with SCPE at 7.86 and 15.72 g/kg BW for 8 weeks, body weight gains were reduced by 18.23% and 19.37%. SCPE could decrease fasting blood glucose, cut down the lipid accumulation in serum and liver, lessen oxidative injury and inflammation in obesity mice. Moreover, SCPE could effectively inhibit the formation of NAFLD by inhibition of LXR-α, SREBP-1c and FAS genes expression, and modulate the structural alteration of gut microbiota in obesity mice. These findings suggested that SCPE could attenuate the features of the metabolism syndrome in obesity mice, which can be used to prevent obesity and NAFLD of human beings.
Neonatal Intracranial Hemorrhage with a Dramatic Outcome Due to Maternal Anti CD36 Antibodies
Authors: Bertrand Gérald , Renac Virginie , Lefaix Marie-Christine , Nivet Carl , Trudel Elise , Richard Lucie
Published: February 2019

Fetal/neonatal allo-immune thrombocytopenia (FNAIT) results from maternal immunization against fetal platelet-specific antigens (HPA) inherited from the father. Most cases involve HPA located on glycoproteins (GP) IbIX, IaIIa and IIbIIIa. Iso-immunizations can also occur in the absence of expression of membrane proteins, such as GPIIb or GPIIIa in Glanzmann patients. CD36 (also called glycoprotein GPIV) deficiency is observed in 3 to 5% of Asian and African populations. We report here the case of a 41-year-old Canadian woman originated from Africa, who delivered a male dead new-born at 39 weeks of gestation. A massive intracranial haemorrhage was identified as being the obvious cause of death. No platelet antibody against GPIbIX, IaIIa, and IIbIIIa was identified by the gold-standard Monoclonal Antibody-specific Immobilization of Platelet Antigens (MAIPA) assay. Surprisingly, anti CD36 iso-antibodies were identified in the maternal serum with a new bead-based multiplex assay. The CD36 gene was sequenced for both parents, and a mutation was identified on Exon 10 of the mother’s CD36 gene, which was absent for the father: NM_000072.3:c.975T>G inducing a STOP codon at position 325 of the mature protein. The absence of CD36 expression on the mother’s platelets was confirmed by flow cytometry.
Indirect Assessment of Sedimentation in Hydropower Dams Using MODIS Remote Sensing Images
Authors: Condé Rita de Cássia , Martinez Jean-Michel , Pessotto Marco Aurélio , Villar Raúl , Cochonneau Gérard , Henry Raoul , Lopes Walszon , Nogueira Marcos
Published: February 2019

In this study, we used moderate resolution imaging spectroradiometer (MODIS) satellite images to quantify the sedimentation processes in a cascade of six hydropower dams along a 700-km transect in the Paranapanema River in Brazil. Turbidity field measurement acquired over 10 years were used to calibrate a turbidity retrieval algorithm based on MODIS surface reflectance products. An independent field dataset was used to validate the remote sensing estimates showing fine accuracy (RMSE of 9.5 NTU, r = 0.75, N = 138). By processing 13 years of MODIS images since 2000, we showed that satellite data can provide robust turbidity monitoring over the entire transect and can identify extreme sediment discharge events occurring on daily to annual scales. We retrieved the decrease in the water turbidity as a function of distance within each reservoir that is related to sedimentation processes. The remote sensing-retrieved turbidity decrease within the reservoirs ranged from 2 to 62% making possible to infer the reservoir type and operation (storage versus run-of-river reservoirs). The reduction in turbidity assessed from space presented a good relationship with conventional sediment trapping efficiency calculations, demonstrating the potential use of this technology for monitoring the intensity of sedimentation processes within reservoirs and at large scale.
Modeling the Amplitude Distribution of Radar Sea Clutter
Authors: Angelliaume Sébastien , Rosenberg Luke , Ritchie Matthew
Published: February 2019

Ship detection in the maritime domain is best performed with radar due to its ability to surveil wide areas and operate in almost any weather condition or time of day. Many common detection schemes require an accurate model of the amplitude distribution of radar echoes backscattered by the ocean surface. This paper presents a review of select amplitude distributions from the literature and their ability to represent data from several different radar systems operating from 1 GHz to 10 GHz. These include the K distribution, arguably the most popular model from the literature as well as the Pareto, K+Rayleigh, and the trimodal discrete (3MD) distributions. The models are evaluated with radar data collected from a ground-based bistatic radar system and two experimental airborne radars. These data sets cover a wide range of frequencies (L-, S-, and X-band), and different collection geometries and sea conditions. To guide the selection of the most appropriate model, two goodness of fit metrics are used, the Bhattacharyya distance which measures the overall distribution error and the threshold error which quantifies mismatch in the distribution tail. Together, they allow a quantitative evaluation of each distribution to accurately model radar sea clutter for the purpose of radar ship detection.
Spatial–Temporal Variation of ANPP and Rain-Use Efficiency Along a Precipitation Gradient on Changtang Plateau, Tibet
Authors: Zhao Guangshuai , Liu Min , Shi Peili , Zong Ning , Wang Jingsheng , Wu Jianshuang , Zhang Xianzhou
Published: February 2019

Aboveground net primary productivity (ANPP) and rain-use efficiency (RUE) are important indicators in assessing the response of ecosystems to climate change. In this paper, the Changtang Plateau in the Tibetan Autonomous Region was selected as the study area to analyze the spatial and temporal changes of ANPP and RUE in grassland communities and their response to climate change. The results showed the following:(1) The spatial pattern of ANPP was closely related to rainfall on the Changtang Plateau. The average ANPP over the past 15 years increased gradually from the arid west to the humid east. A consistent pattern was exhibited in different grassland types and climate zones. (2) The RUE was higher at the east and west edges of the Changtang Plateau, especially in the arid west, but was lower in the center. From the perspective of different climatic zones, the average RUE in the southern Tibetan semiarid climate zone and the Ngari arid climate zone was significantly higher than that in other climate zones. However, the average RUE in different grassland types only varied from 0.07 to 0.09 g·m−2·mm−1. The spatial variation in RUE was more distinct in different climatic zones than in different grassland types. (3) Climate change influenced the interannual variation of ANPP and RUE, but the response of ANPP to rainfall showed a significant lag. The interannual change in RUE was negatively correlated with changes in precipitation. (4) In general, a greater area showed a significant increase rather than a decrease in ANPP on the Changtang Plateau, which meant that the grassland condition is improving. The temporal variation patterns of ANPP and RUE in different climate zones were consistent with the overall patterns on the Changtang Plateau, while the variation was not significant in different grassland types.
Gold Nanodisks Plasmonic Array for Hydrogen Sensing at Low Temperature
Authors: Sturaro Marco , Zacco Gabriele , Zilio Pierfrancesco , Surpi Alessandro , Bazzan Marco , Martucci Alessandro
Published: February 2019

We present a novel plasmonic hydrogen sensor consisting of an array of gold nanodisks produced by lithography. The size, height, and spacing of the disks were optimized using finite element simulation to generate a sharp localized surface plasmon resonance peak in the near-infrared wavelength region. The reported results show the possibility of developing an optical gas sensors-based bare Au nanostructures operating at a low temperature.
A Laboratory Study on Non-Invasive Soil Water Content Estimation Using Capacitive Based Sensors
Authors: Orangi Amir , Narsilio Guillermo A. , Ryu Dongryeol
Published: February 2019

Soil water content is an important parameter in many engineering, agricultural and environmental applications. In practice, there exists a need to measure this parameter rather frequently in both time and space. However, common measurement techniques are typically invasive, time-consuming and labour-intensive, or rely on potentially risky (although highly regulated) nuclear-based methods, making frequent measurements of soil water content impractical. Here we investigate in the laboratory the effectiveness of four new low-cost non-invasive sensors to estimate the soil water content of a range of soil types. While the results of each of the four sensors are promising, one of the sensors, herein called the “AOGAN” sensor, exhibits superior performance, as it was designed based on combining the best geometrical and electronic features of the other three sensors. The performance of the sensors is, however, influenced by the quality of the sensor-soil coupling and the soil surface roughness. Accuracy was found to be within 5% of volumetric water content, considered sufficient to enable higher spatiotemporal resolution contrast for mapping of soil water content.
Calibration of Beacons for Indoor Environments based on a Digital Map and Heuristic Information
Authors: Gualda David , Ureña Jesús , Alcalá José , Santos Carlos
Published: February 2019

This paper proposes an algorithm for calibrating the position of beacons which are placed on the ceiling of an indoor environment. In this context, the term calibration is used to estimate the position coordinates of a beacon related to a known reference system in a map. The positions of a set of beacons are used for indoor positioning purposes. The operation of the beacons can be based on different technologies such as radiofrequency (RF), infrared (IR) or ultrasound (US), among others. In this case we are interested in the positions of several beacons that compose an Ultrasonic Local Positioning System (ULPS) placed on different strategic points of the building. The calibration proposal uses several distances from a beacon to the neighbor walls measured by a laser meter. These measured distances, the map of the building in a vector format and other heuristic data (such as the region in which the beacon is located, the approximate orientation of the distance measurements to the walls and the equations in the map coordinate system of the line defining these walls) are the inputs of the proposed algorithm. The output is the best estimation of the position of the beacon. The process is repeated for all the beacons. To find the best estimation of the position of the beacons we have implemented a numerical minimization based on the use of a Genetic Algorithm (GA) and a Harmony Search (HS) methods. The proposal has been validated with simulations and real experiments, obtaining the positions of the beacons and an estimation of the error associated that depends on which walls (and the angle of incidence of the laser) are selected to make the distance measurements.
Sustainability Assessment: Exploring the Frontiers and Paradigms of Indicator Approaches
Authors: Ramos Tomás B.
Published: February 2019

Sustainability assessment approaches could support all levels of decision-making and policy processes (including strategies, policies, plans, programs, projects, and activities/operations), thus improving the management of natural and human systems. Sustainability Indicators (SIs) have been extensively used to assess and communicate the progress toward sustainable development. However, despite all the SI initiatives and the well-known advantages and popularity, several risks have been pointed out, so there is a need to rethink the current state of SIs and build visions that could reshape the indicator reality. The main goal of this research is to develop a constructive debate around the possible futures and paths of SIs’, by conducting a critical analysis of a set of challenges and opportunities identified by the literature. This was explored through a critical perspective and viewpoint article that discusses what could be some of the new frontiers and paradigms in SIs. Exploratory research supported by a combination of methods was conducted, consisting of a search of the literature and qualitative document analysis, followed by an assessment procedure based upon an evaluation ranking scale. The classification scale integrated three main criteria of valuation: Relevancy, feasibility, and societal impacts. The findings showed that most of the challenges and opportunities analyzed are old and mainly technically oriented, with a low potential impact on society, including end-users and practitioners. The majority of the challenges have low-to-medium feasibility, showing that there would be difficulty in implementing them, and so they should be improved or redesigned. A set of key questions on SIs’ futures is proposed, aiming to represent a critical view of the relevant challenges and opportunities analyzed, but underpinned and observed from a crosscutting angle, represented by the societal role. The SI research community should be ready to adapt ways of thinking and doing, responding to new global and local paradigms and using transdisciplinary collaborative scientific development and innovation as the foundations for the change process, wherein communities and the individual have central roles to play.
Modeling with Stakeholders for Transformative Change
Authors: van Bruggen Anne , Nikolic Igor , Kwakkel Jan
Published: February 2019

Coherent responses to important problems such as climate change require involving a multitude of stakeholders in a transformative process leading to development of policy pathways. The process of coming to an agreement on policy pathways requires critical reflection on underlying system conceptualizations and commitment to building capacity in all stakeholders engaged in a social learning process. Simulation models can support such processes by providing a boundary object or negotiating artifact that allows stakeholders to deliberate through a multi-interpretable, consistent, transparent, and verifiable representation of reality. The challenge is how to structure the transdisciplinary process of involving stakeholders in simulation modeling and how to know when such a process can be labeled as transformative. There is a proliferation of approaches for this across disciplines, of which this article identifies Group Model Building, Companion Modeling, Challenge-and-Reconstruct Learning, and generic environmental modeling as the most prominent. This article systematically reviews relevant theories, terminology, principles, and methodologies across these four approaches to build a framework that can facilitate further learning. The article also provides a typology of approaches to modeling with stakeholders. It distinguishes transformative approaches that involve stakeholders from representative, instrumental and nominal forms. It is based on an extensive literature review, supported by twenty-three semi-structured interviews with participatory and non-participatory modelers. The article brings order into the abundance of conceptions of transformation, the role of simulation models in transformative change processes, the role of participation of stakeholders, and what type of approaches to modeling with stakeholders are befitting in the development of policy pathways.
36th Cordoba Guitar Festival: Spectator Analysis Using Structural Equation Modelling (SEM)
Authors: Fruet-Cardozo Juan , Pérez-Gálvez Jesús , Jara-Alba Carol , Gómez-Casero Gema
Published: February 2019

The Cordoba Guitar Festival is one of the most important cultural events in Spain. This article analyses the musical preferences, satisfaction, attitudinal loyalty, and behavioural loyalty of spectators who attended the 36th festival held in July 2016, as well as the festival’s economic impact on the city. These characteristics of the public give rise to the four hypotheses of this study. To achieve this aim, a structural equation model (SEM) was used. The results −χ2 = 27.79, with the p-value found to be p = P (χ2 ≥ 27.79) = 0.224—show the goodness-of-fit of the model and indicate that the observed data do fit the expected dataset. The results therefore allow us to accept the proposed hypotheses concerning the influence of different latent factors, such as musical preferences, loyalty, and satisfaction with the festival. This is the main focus with which the present study has been written. Regarding the economic impact, the Festival raised 1.2 million euros, that is, slightly more than 73,000 euros per day. The amount, although not very high, is important as additional income for the city. The conclusions show the need to consider the preferences of the spectator as an essential tool to enable the continuation and longevity of the Cordoba Guitar Festival—an important cultural destination.
The Mobile Emotional Intelligence Test (MEIT): An Ability Test to Assess Emotional Intelligence at Work
Authors: Sanchez-Gomez Martin , Breso Edgar
Published: February 2019

The present study analyzes the Mobile Emotional Intelligence Test (MEIT), a new ability-test to assess emotional intelligence (EI) in a digital way. Taking into account the importance of emotional competencies in the study of employees’ wellbeing and performance, the instrument tested is based on the most supported ability model (Four-branch Mayer and Salovey Model), and it evaluates emotional capacity through nine different emotional tasks. A total of 1549 participants (841 women and 708 men) with an average age of 27.77 (SD = 8.75) fulfilled the MEIT, consisting of 42 items. The score on the test is based on expert judgments: professional psychologists and emotional intelligence specialists. In addition to the MEIT test, a series of questionnaires was used to assess relevant constructs which research has shown to be related to EI (general intelligence, personality traits, and life satisfaction); besides, another measure of emotional intelligence trait (TMMS-24) was included. The results showed that the MEIT is a reliable and valid test that is useful for both scientific research and individual assessment. Statistical analysis provides evidence of the reliability and validity of the three-factor structure of the questionnaire. Moreover, internal consistency measures were high. In line with previous studies, MEIT maintains the expected relationships with the rest of the constructs studied. Finally, the limitations of the present study and the need for future research on emotional intelligence assessment are discussed.