bluuebunny/crossref_metadata_2025 huggingface dataset:
Disruption of GPR35 Signaling in Bone Marrow-Derived Cells Does Not Influence Vascular Inflammation and Atherosclerosis in Hyperlipidemic Mice
Authors: Baumgartner Roland , Casagrande Felipe B. , Mikkelsen Randi B. , Berg Martin , Polyzos Konstantinos A. , Forteza Maria J. , Arora Aastha , Schwartz Thue W. , Hjorth Siv A. , Ketelhuth Daniel F. J.
Published: June 2021

G-protein-coupled receptor-35 (GPR35) has been identified as a receptor for the tryptophan metabolite kynurenic acid (KynA) and suggested to modulate macrophage polarization in metabolic tissues. Whether GPR35 can influence vascular inflammation and atherosclerosis has however never been tested. Lethally irradiated LdlrKO mice were randomized to receive GPR35KO or wild type (WT) bone marrow transplants and fed a high cholesterol diet for eight weeks to develop atherosclerosis. GPR35KO and WT chimeric mice presented no difference in the size of atherosclerotic lesions in the aortic arch (2.37 ± 0.58% vs. 1.95 ± 0.46%, respectively) or in the aortic roots (14.77 ± 3.33% vs. 11.57 ± 2.49%, respectively). In line with these data, no changes in the percentage of VCAM-1+, IAb + cells, and CD3+ T cells, as well as alpha smooth muscle cell actin expression, was observed between groups. Interestingly, the GPR35KO group presented a small but significant increase in CD68+ macrophage infiltration in the plaque. However, in vitro culture experiments using bone marrow-derived macrophages from both groups indicated that GPR35 plays no role in modulating the secretion of major inflammatory cytokines. Our study indicates that GPR35 expression does not play a direct role in macrophage activation, vascular inflammation, and the development of atherosclerosis.
Hydraulic Integrated Interconnected Regenerative Suspension: Modeling and Characteristics Analysis
Authors: Guo Sijing , Chen Liang , Wang Xikai , Zou Junyi , Hu Sanbao
Published: June 2021

A novel suspension system, the hydraulic integrated interconnected regenerative suspension (HIIRS), has been proposed recently. This paper demonstrates the vibration and energy harvesting characteristics of the HIIRS. The HIIRS model is established as a set of coupled, frequency-dependent equations with the hydraulic impedance method. The mechanical–fluid boundary condition in the double-acting cylinders is modelled as an external force on the mechanical system and a moving boundary on the fluid system. By integrating the HIIRS into a half car model, its free and forced vibration analyses are conducted and compared with an equivalent traditional off-road vehicle. Results show that the natural frequency and the damping ratio of the HIIRS-equipped vehicle are within a proper range of a normal off-road vehicle. The root mean square values of the bounce and roll acceleration of the HIIRS system are, respectively, 64.62 and 11.21% lower than that of a traditional suspension. The average energy harvesting power are 186.93, 417.40 and 655.90 W at the speeds of 36, 72 and 108 km/h for an off-road vehicle on a Class-C road. The results indicate that the HIIRS system can significantly enhance the vehicle dynamics and harvest the vibration energy simultaneously.
Evaluation of Probiotic Properties of Pediococcus acidilactici M76 Producing Functional Exopolysaccharides and Its Lactic Acid Fermentation of Black Raspberry Extract
Authors: Song Young-Ran , Lee Chan-Mi , Lee Seon-Hye , Baik Sang-Ho
Published: June 2021

This study aimed to determine the probiotic potential of Pediococcus acidilactici M76 (PA-M76) for lactic acid fermentation of black raspberry extract (BRE). PA-M76 showed outstanding probiotic properties with high tolerance in acidic GIT environments, broad antimicrobial activity, and high adhesion capability in the intestinal tract of Caenorhabditis elegans. PA-M76 treatment resulted in significant increases of pro-inflammatory cytokine mRNA expression in macrophages, indicating that PA-M76 elicits an effective immune response. When PA-M76 was used for lactic acid fermentation of BRE, an EPS yield of 1.62 g/L was obtained under optimal conditions. Lactic acid fermentation of BRE by PA-M76 did not significantly affect the total anthocyanin and flavonoid content, except for a significant increase in total polyphenol content compared to non-fermented BRE (NfBRE). However, fBRE exhibited increased DPPH radical scavenging activity, linoleic acid peroxidation inhibition rate, and ABTS scavenging activity of fBRE compared to NfBRE. Among the 28 compounds identified in the GC-MS analysis, esters were present as the major groups. The total concentration of volatile compounds was higher in fBRE than that in NfBRE. However, the undesirable flavor of terpenes decreased. PA-M76 might be useful for preparing functionally enhanced fermented beverages with a higher antioxidant activity of EPS and enhanced flavors.
Testing Trace-Element Distribution and the Zr-Based Thermometry of Accessory Rutile from Chromitite
Authors: Zaccarini Federica , Garuti Giorgio , Luvizotto George L. , de Melo Portella Yuri , Singh Athokpam K.
Published: June 2021

Trace element distribution and Zr-in-rutile temperature have been investigated in accessory rutile from stratiform (UG2, Merensky Reef, Jacurici), podiform (Loma Peguera), and metamorphic chromitites in cratonic shields (Cedrolina, Nuasahi). Rutile from chromitite has typical finger-print of Cr-V-Nb-W-Zr, whose relative abundance distinguishes magmatic from metamorphic chromitite. In magmatic deposits, rutile precipitates as an intercumulus phase, or forms by exsolution from chromite, between 870 °C and 540 °C. The Cr-V in rutile reflects the composition of chromite, both Nb and Zr are moderately enriched, and W is depleted, except for in Jacurici, where moderate W excess was a result of crustal contamination of the mafic magma. In metamorphic deposits, rutile forms by removal of Ti-Cr-V from chromite during metamorphism between 650 °C and 400 °C, consistent with greenschist-amphibolite facies, and displays variable Cr-Nb, low V-Zr, and anomalous enrichment in W caused by reaction with felsic fluids emanating from granitoid intrusions. All deposits, except Cedrolina, contain Rutile+PGM composite grains (<10 µm) locked in chromite, possibly representing relics of orthomagmatic assemblages. The high Cr-V content and the distinctive W-Nb-Zr signature that typifies accessory rutile in chromitite provide a new pathfinder to trace the provenance of detrital rutile in placer deposits.
Talc Flotation—An Overview
Authors: Ann Bazar July , Rahimi Milad , Fathinia Siavash , Jafari Mohammad , Chipakwe Vitalis , Chehreh Chelgani Saeed
Published: June 2021

Talc is a naturally hydrophobic gangue mineral in most sulfide ores. However, talc has vast applications in the cosmetics, paper, and paint industries due to its high chemical stability, and its demand continues to grow. Since flotation is the most effective beneficiation technique for upgrading sulfides, the high hydrophobicity of talc has made its selective separation challenging. This paper explored the different properties of talc and the different factors that affect its flotation separation performance as a proven versatile beneficiation technique. Surface properties, zeta potential measurements, contact angles, and other factors affecting the talc flotation efficiency were discussed in detail. It was observed that the surface face/edge ratio (particle size) has a direct relationship with the level of talc hydrophobicity. Talc surfaces are negatively charged in a wide pH range (pH 2–12). Different depressants have already been studied; however, most of them showed low selectivity. The addition of ions such as Ca2+ could enhance talc depression. Pretreatment methods such as ultrasonic and thermal treatments were reported to decrease the talc floatability. It was demonstrated that the development of new selective depressants or pretreatment options for talc flotation requires attention in future investigations to improve its selective separation.
Development of a New Solver to Model the Fish-Hook Effect in a Centrifugal Classifier
Authors: Betz Michael , Nirschl Hermann , Gleiss Marco
Published: June 2021

Centrifugal air classifiers are often used for classification of particle gas flows in the mineral industry and various other sectors. In this paper, a new solver based on the multiphase particle-in-cell (MP-PIC) method, which takes into account an interaction between particles, is presented. This makes it possible to investigate the flow process in the classifier in more detail, especially the influence of solid load on the flow profile and the fish-hook effect that sometimes occurs. Depending on the operating conditions, the fish-hook sometimes occurs in such apparatus and lead to a reduction in classification efficiency. Therefore, a better understanding and a representation of the fish-hook in numerical simulations is of great interest. The results of the simulation method are compared with results of previous simulation method, where particle–particle interactions are neglected. Moreover, a validation of the numerical simulations is carried out by comparing experimental data from a laboratory plant based on characteristic values such as pressure loss and classification efficiency. The comparison with experimental data shows that both methods provide similar good values for the classification efficiency d50; however, the fish-hook effect is only reproduced when particle-particle interaction is taken into account. The particle movement prove that the fish-hook effect is due to a strong concentration accumulation in the outer area of the classifier. These particle accumulations block the radial transport of fine particles into the classifier, which are then entrained by coarser particles into the coarse material.
Influence of Host Marble Rocks on the Formation of Intrusive Alkaline Rocks and Carbonatites of Sangilen (E. Siberia, Russia)
Authors: Nikiforov Anatoly V. , Dubinina Elena O. , Polyakov Nikolay A. , Sugorakova Amina M. , Khertek Aylan K.
Published: June 2021

The study of the O and C isotope composition of calcite from nepheline syenites, ijolites and carbonatites of the Chik intrusion and the intrusions of the Erzin–Tarbagatay group of Sangilen (Eastern Siberia, Russia) showed derivation from alkaline melts enriched with a carbonate component from the host marbleized sedimentary rocks. The calculations showed that about 40% of the initial mass of carbonates involved in the interaction with silicate melts have remained after decarbonation. During the assimilation of the carbonate, an oxygen isotope exchange took place between the residual carbonate material and the silicate phase. Crystallization products of such hybrid magmas are carbonatite veins, calcite-rich nepheline rocks and their pegmatites with a calcite core.
How Climatic Seasons of the Amazon Biome Affect the Aromatic and Bioactive Profiles of Fermented and Dried Cocoa Beans?
Authors: Gaspar Daniela Pinheiro , Chagas Junior Gilson Celso Albuquerque , de Aguiar Andrade Eloisa Helena , Nascimento Lidiane Diniz do , Chisté Renan Campos , Ferreira Nelson Rosa , Martins Luiza Helena da Silva , Lopes Alessandra Santos
Published: June 2021

In addition to the vast diversity of fauna and flora, the Brazilian Amazon has different climatic periods characterized by periods with greater and lesser rainfall. The main objective of this research was to verify the influence of climatic seasons in the Brazilian Amazon (northeast of Pará state) concerning the aromatic and bioactive profiles of fermented and dried cocoa seeds. About 200 kg of seeds was fermented using specific protocols of local producers. Physicochemical analyzes (total titratable acidity, pH, total phenolic compounds, quantification of monomeric phenolics and methylxanthines) and volatile compounds by GC-MS were carried out. We observed that: in the summer, the highest levels of aldehydes were identified, such as benzaldehyde (6.34%) and phenylacetaldehyde (36.73%), related to the fermented cocoa and honey aromas, respectively; and a total of 27.89% of this same class was identified during winter. There were significant differences (p ≤ 0.05, Tukey test) in the profile of bioactive compounds (catechin, epicatechin, caffeine, and theobromine), being higher in fermented almonds in winter. This study indicates that the climatic seasons in the Amazon affect the aromatic and bioactive profiles and could produce a new identity standard (summer and winter Amazon) for the cocoa almonds and their products.
Isolation and Purification of Fucoxanthin from Brown Seaweed Sargassum horneri Using Open ODS Column Chromatography and Ethanol Precipitation
Authors: Ye Yuemei , Sun Jingwen , Wang Liting , Zhu Junwang , Cui Wei , Hou Hongyan , Zhang Jinrong , Zhou Chengxu , Yan Xiaojun
Published: June 2021

As an abundant marine xanthophyll, fucoxanthin (FX) exhibits a broad range of biological activities. The preparation of high-purity FX is in great demand, however, most of the available methods require organic solvents which cannot meet the green chemistry standard. In the present study, a simple and efficient purification approach for the purification of FX from the brown seaweed Sargassum horneri was carried out. The FX-rich ethanol extract was isolated by octadecylsilyl (ODS) column chromatography using ethanol–water solvent as a gradient eluent. The overwhelming majority of FX was successfully eluted by the ethanol–water mixture (9:1, v/v), with a recovery rate of 95.36%. A parametric study was performed to optimize the aqueous ethanol precipitation process by investigating the effects on the purity and recovery of FX. Under the optimal conditions, the purity of FX was 91.07%, and the recovery rate was 74.98%. Collectively, the eco-friendly method was cost-efficient for the purification of FX. The developed method provides a potential approach for the large-scale production of fucoxanthin from the brown seaweed Sargassum horneri.
4,7-Disubstituted 7H-Pyrrolo[2,3-d]pyrimidines and Their Analogs as Antiviral Agents against Zika Virus
Authors: Soto-Acosta Ruben , Jung Eunkyung , Qiu Li , Wilson Daniel J. , Geraghty Robert J. , Chen Liqiang
Published: June 2021

Discovery of compound 1 as a Zika virus (ZIKV) inhibitor has prompted us to investigate its 7H-pyrrolo[2,3-d]pyrimidine scaffold, revealing structural features that elicit antiviral activity. Furthermore, we have demonstrated that 9H-purine or 1H-pyrazolo[3,4-d]pyrimidine can serve as an alternative core structure. Overall, we have identified 4,7-disubstituted 7H-pyrrolo[2,3-d]pyrimidines and their analogs including compounds 1, 8 and 11 as promising antiviral agents against flaviviruses ZIKV and dengue virus (DENV). While the molecular target of these compounds is yet to be elucidated, 4,7-disubstituted 7H-pyrrolo[2,3-d]pyrimidines and their analogs are new chemotypes in the design of small molecules against flaviviruses, an important group of human pathogens.
Novel Spray Dried Algae-Rosemary Particles Attenuate Pollution-Induced Skin Damage
Authors: Hoskin Roberta , Pambianchi Erika , Pecorelli Alessandra , Grace Mary , Therrien Jean-Philippe , Valacchi Giuseppe , Lila Mary Ann
Published: June 2021

The present study investigated the effect of spray-dried algae-rosemary particles against pollution-induced damage using ex-vivo human biopsies exposed to diesel engine exhaust (DEE). For this, the complexation of hydroalcoholic rosemary extract with Chlorella (RCH) and Spirulina (RSP) protein powders was conducted. The process efficiency and concentration of rosmarinic acid (RA), carnosic acid (CA), and carnosol (CR) phenolic compounds of both products were compared. The RSP spray-dried production was more efficient, and RSP particles presented higher CR and CA and similar RA concentrations. Therefore, spray-dried RSP particles were prioritized for the preparation of a gel formulation that was investigated for its ability to mitigate pollution-induced skin oxinflammatory responses. Taken altogether, our ex-vivo data clearly demonstrated the ability of RSP gel to prevent an oxinflammatory phenomenon in cutaneous tissue by decreasing the levels of 4-hydroxynonenal protein adducts (4HNE-PA) and active matrix metalloproteinase-9 (MMP-9) as well as by limiting the loss of filaggrin induced by DEE exposure. Our results suggest that the topical application of spirulina-rosemary gel is a good approach to prevent pollution-induced skin aging/damage.
Catalytic Activity of Thermolyzed [Co(NH3)6][Fe(CN)6] in CO Hydrogenation Reaction
Authors: Gosteva Alevtina N. , Kulikova Mayya V. , Semushina Yulya P. , Chudakova Mariya V. , Tsvetov Nikita S. , Semushin Vasilii V.
Published: June 2021

Currently, the processes of obtaining synthetic liquid hydrocarbons and oxygenates are very relevant. Fischer-Tropsch synthesis (FTS) is the most important step in these processes. The products of thermal destruction in argon of the mixture [Co(NH3)6][Fe(CN)6] and Al(OH)3 were used as catalysts for CO hydrogenation. The resulting compositions were studied using powder X-ray diffraction, IR spectroscopy, elemental analysis, SEM micrographs. The specific surface area, pore and particle size distributions were determined. It was determined that the DCS-based catalysts were active in the high-temperature Fischer-Tropsch synthesis. The effect of aluminum in the catalyst composition on the distribution of reaction products was revealed.
Optimization of Ultrasound Assisted Extraction of Bioactive Compounds from Apple Pomace
Authors: Egüés Itziar , Hernandez-Ramos Fabio , Rivilla Iván , Labidi Jalel
Published: June 2021

In the present work, the optimization of the extraction of antioxidant compounds from apple pomace using ultrasound technology as an environmentally friendly and intensification process was developed. Different sonication powers, extraction temperatures and extraction times were studied and their influence on extraction yield and characteristics of the extracted samples (total phenolic compounds, flavonoid content and antioxidant capacity) are presented. The elaborated experimental design and the analysis of Pareto and response surface diagrams allowed us to determine the optimal extraction conditions. The conditions that allow the maximum extraction of phenolic compounds were found at 20 min, 90 °C and 50% ultrasound amplitude. Nevertheless, at these conditions, the antioxidant capacity measured by DPPH decreased in the extracted samples.
The Influence of Temperature, Storage Conditions, pH, and Ionic Strength on the Antioxidant Activity and Color Parameters of Rowan Berry Extracts
Authors: Cristea Elena , Ghendov-Mosanu Aliona , Patras Antoanela , Socaciu Carmen , Pintea Adela , Tudor Cristina , Sturza Rodica
Published: June 2021

Recent trends in the food industry combined with novel methods in agriculture could transform rowan into a valuable raw material with potential technological applications. Thus, the aim of this research was to investigate the content of bioactive compounds in its fruits and to assess the color and antioxidant stability of the extracts prepared from such fruits during various thermal treatments and at different pH and ionic strength values. Various spectrophotometric methods, HPLC, and capillary electrophoresis were used to quantify the concentrations of bioactive compounds—polyphenols, carotenoids, organic acids, and to assess antioxidant activity and color. The results show that rowan berries contain circa 1.34–1.47 g/100 g of polyphenols among which include catechin, epicatechin, ferulic acid methyl ester, procyanidin B1, etc.; ca 21.65 mg/100 g of carotenoids including zeaxanthin, β-cryptoxanthin, all-trans-β-carotene, and various organic acids such as malic, citric, and succinic, which result in a high antioxidant activity of 5.8 mmol TE/100 g. Results also showed that antioxidant activity exhibited high stability when the extract was subjected to various thermal treatments, pHs, and ionic strengths, while color was mainly impacted negatively when a temperature of 100 °C was employed. This data confirms the technological potential of this traditional, yet often overlooked species.
Development and Validation of a Method for Quantification of Favipiravir as COVID-19 Management in Spiked Human Plasma
Authors: Hailat Mohammad , Al-Ani Israa , Hamad Mohammed , Zakareia Zainab , Abu Dayyih Wael
Published: June 2021

In the current work, a simple, economical, accurate, and precise HPLC method with UV detection was developed to quantify Favipiravir (FVIR) in spiked human plasma using acyclovir (ACVR) as an internal standard in the COVID-19 pandemic time. Both FVIR and ACVR were well separated and resolved on the C18 column using the mobile phase blend of methanol:acetonitrile:20 mM phosphate buffer (pH 3.1) in an isocratic mode flow rate of 1 mL/min with a proportion of 30:10:60 %, v/v/v. The detector wavelength was set at 242 nm. Maximum recovery of FVIR and ACVR from plasma was obtained with dichloromethane (DCM) as extracting solvent. The calibration curve was found to be linear in the range of 3.1–60.0 µg/mL with regression coefficient (r2) = 0.9976. However, with acceptable r2, the calibration data’s heteroscedasticity was observed, which was further reduced using weighted linear regression with weighting factor 1/x. Finally, the method was validated concerning sensitivity, accuracy (Inter and Intraday’s % RE and RSD were 0.28, 0.65 and 1.00, 0.12 respectively), precision, recovery (89.99%, 89.09%, and 90.81% for LQC, MQC, and HQC, respectively), stability (% RSD for 30-day were 3.04 and 1.71 for LQC and HQC, respectively at −20 °C), and carry-over US-FDA guidance for Bioanalytical Method Validation for researchers in the COVID-19 pandemic crisis. Furthermore, there was no significant difference for selectivity when evaluated at LLOQ concentration of 3 µg/mL of FVIR and relative to the blank.
High-Throughput Fluorescent Assay for Inhibitor Screening of Proteases from RNA Viruses
Authors: Cihlova Bara , Huskova Andrea , Böserle Jiri , Nencka Radim , Boura Evzen , Silhan Jan
Published: June 2021

Spanish flu, polio epidemics, and the ongoing COVID-19 pandemic are the most profound examples of severe widespread diseases caused by RNA viruses. The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands affordable and reliable assays for testing antivirals. To test inhibitors of viral proteases, we have developed an inexpensive high-throughput assay based on fluorescent energy transfer (FRET). We assayed an array of inhibitors for papain-like protease from SARS-CoV-2 and validated it on protease from the tick-borne encephalitis virus to emphasize its versatility. The reaction progress is monitored as loss of FRET signal of the substrate. This robust and reproducible assay can be used for testing the inhibitors in 96- or 384-well plates.
Characterization of Phase I Hepatic Metabolites of Anti-Premature Ejaculation Drug Dapoxetine by UHPLC-ESI-Q-TOF
Authors: Skibiński Robert , Trawiński Jakub , Gawlik Maciej
Published: June 2021

Determination of the metabolism pathway of xenobiotics undergoing the hepatic pass is a crucial aspect in drug development since the presence of toxic biotransformation products may result in significant side effects during the therapy. In this study, the complete hepatic metabolism pathway of dapoxetine established according to the human liver microsome assay with the use of a high-resolution LC–MS system was described. Eleven biotransformation products of dapoxetine, including eight metabolites not reported in the literature so far, were detected and identified. N-dealkylation, hydroxylation, N-oxidation and dearylation were found to be the main metabolic reactions for the investigated xenobiotic. In silico analysis of toxicity revealed that the reaction of didesmethylation may contribute to the increased carcinogenic potential of dapoxetine metabolites. On the other hand, N-oxidation and aromatic hydroxylation biotransformation reactions possibly lead to the formation of mutagenic compounds.
Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors
Authors: Drača Dijana , Marković Milan , Gozzi Marta , Mijatović Sanja , Maksimović-Ivanić Danijela , Hey-Hawkins Evamarie
Published: June 2021

Gliomas and glioblastomas are very aggressive forms of brain tumors, prone to the development of a multitude of resistance mechanisms to therapeutic treatments, including cytoprotective autophagy. In this work, we investigated the role and mechanism of action of the combination of a ruthenacarborane derivative with 8-hydroxyquinoline (8-HQ), linked via an ester bond (complex 2), in rat astrocytoma C6 and human glioma U251 cells, in comparison with the two compounds alone, i.e., the free carboxylic acid (complex 1) and 8-HQ, and their non-covalent combination ([1 + 8-HQ], in 1:1 molar ratio). We found that only complex 2 was able to significantly affect cellular viability in glioma U251 cells (IC50 11.4 μM) via inhibition of the autophagic machinery, most likely acting at the early stages of the autophagic cascade. Contrary to 8-HQ alone, complex 2 was also able to impair cellular viability under conditions of glucose deprivation. We thus suggest different mechanisms of action of ruthenacarborane complex 2 than purely organic quinoline-based drugs, making complex 2 a very attractive candidate for evading the known resistances of brain tumors to chloroquine-based therapies.
New Insights into the Degradation Path of Deltamethrin
Authors: Aiello Federica , Simons Marcel G. , van Velde Jan W. , Dani Paulo
Published: June 2021

Pyrethroids are among the insecticidal compounds indicated by the World Health Organization for mitigation of vector-borne diseases. Active deltamethrin (with chiral configuration α-S,1-R-cis) is one of the most effective pyrethroids characterized by low toxicity to humans, and it is currently tested as active ingredient for insecticidal paints. Nevertheless, several degradation processes can occur and affect the insecticidal efficacy in the complex paint matrix. In the present study, a detailed NMR analysis of deltamethrin stability has been carried out under stress conditions, mimicking a water-based insecticidal paint environment. Two novel by-products, having a diastereomeric relationship, were identified and their structure was elucidated by combining NMR, HPLC, GC-MS, and ESI-MS analyses. These compounds are the result from a nucleophilic addition involving deltamethrin and one of its major degradation products, 3-phenoxybenzaldehyde. Given the known toxicity of the aldehyde, this reaction could represent a way to reduce its concentration into the matrix. On the other hand, the toxicology of these compounds towards humans should be addressed, as their presence may adversely affect the performance of deltamethrin-containing products.
Gastroprotective Activities of Ethanol Extract of Black Rice Bran (Oryza sativa L.) in Rats
Authors: Tonchaiyaphum Peerachit , Arpornchayanon Warangkana , Khonsung Parirat , Chiranthanut Natthakarn , Pitchakarn Pornsiri , Kunanusorn Puongtip
Published: June 2021

Black rice is a type of rice in the Oryza sativa L. species. There are numerous reports regarding the pharmacological actions of black rice bran, but scientific evidence on its gastroprotection is limited. This study aimed to evaluate the gastroprotective activities of black rice bran ethanol extract (BRB) from the Thai black rice variety Hom Nil (O. sativa L. indica) as well as its mechanisms of action, acute oral toxicity in rats, and phytochemical screening. Rat models of gastric ulcers induced by acidified ethanol, indomethacin, and restraint water immersion stress were used. After pretreatment with 200, 400, and 800 mg/kg of BRB in test groups, BRB at 800 mg/kg significantly inhibited the formation of gastric ulcers in all gastric ulcer models, and this inhibition seemed to be dose dependent in an indomethacin-induced gastric ulcer model. BRB could not normalize the amount of gastric wall mucus, reduce gastric volume and total acidity, or increase gastric pH. Although BRB could not increase NO levels in gastric tissue, the tissue MDA levels could be normalized with DPPH radical scavenging activity. These results confirm the gastroprotective activities of BRB with a possible mechanism of action via antioxidant activity. The major phytochemical components of BRB comprise carotenoid derivatives with the presence of phenolic compounds. These components may be responsible for the gastroprotective activities of BRB. The 2000 mg/kg dose of oral BRB showed no acute toxicity in rats and confirmed, in part, the safe uses of BRB.
Invasive Plant Species Biomass—Evaluation of Functional Value
Authors: Peter Anamarija , Žlabur Jana Šic , Šurić Jona , Voća Sandra , Purgar Dubravka Dujmović , Pezo Lato , Voća Neven
Published: June 2021

Invasive plant species (IAS), with their numerous negative ecological, health, and economic impacts, represent one of the greatest conservation challenges in the world. Reducing the negative impacts and potentially exploiting the biomass of these plant species can significantly contribute to sustainable management, protect biodiversity, and create a healthy environment. Therefore, the main objective of this study was to evaluate the nutritional potential, phytochemical status, and antioxidant capacity of nine alien invasive plant species: Abutilon theophrasti, Amaranthus retroflexus, Ambrosia artemisiifolia, Datura stramonium, Erigeron annuus, Galinsoga ciliata, Reynoutria japonica, Solidago gigantea, and Sorghum halepense. Multivariate statistical methods such as cluster and PCA were performed to determine possible connections and correlations among selected IAS depending on the phytochemical content. According to the obtained results, R. japonica was notable with the highest content of vitamin C (38.46 mg/100 g FW); while E. annuus (1365.92 mg GAE/100 g FW) showed the highest values of total polyphenolic compounds. A. retroflexus was characterized by the highest content of total chlorophylls (0.26 mg/g) and antioxidant capacity (2221.97 µmol TE/kg). Therefore, it can be concluded that the selected IAS represent nutrient-rich plant material with significant potential for the recovering of bioactive compounds.
Dynamics of Phloridzin and Related Compounds in Four Cultivars of Apple Trees during the Vegetation Period
Authors: Táborský Jan , Sus Josef , Lachman Jaromír , Šebková Barbora , Adamcová Anežka , Šatínský Dalibor
Published: June 2021

Apple trees (Malus domestica Borgh) are a rich source of dihydrochalcones, phenolic acids and flavonoids. Considering the increasing demand for these phytochemicals with health-benefitting properties, the objective of this study was to evaluate the profile of the main bioactive compounds—phloridzin, phloretin, chlorogenic acid and rutin—in apple tree bark, leaves, flower buds and twigs. The variety in the phenolic profiles of four apple tree cultivars was monitored during the vegetation period from March to September using chromatography analysis. Phloridzin, the major glycoside of interest, reached the highest values in the bark of all the tested cultivars in May (up to 91.7 ± 4.4 mg g−1 of the dried weight (DW), cv. ‘Opal’). In the leaves, the highest levels of phloridzin were found in cv. ‘Opal’ in May (82.5 ± 22.0 mg g−1 of DW); in twigs, the highest levels were found in cv. ‘Rozela’ in September (52.4 ± 12.1 mg g−1 of DW). In the flower buds, the content of phloridzin was similar to that in the twigs. Aglycone phloretin was found only in the leaves in relatively low concentrations (max. value 2.8 ± 1.4 mg g−1 of DW). The highest values of rutin were found in the leaves of all the tested cultivars (10.5 ± 2.9 mg g−1 of DW, cv. ‘Opal’ in September); the concentrations in the bark and twigs were much lower. The highest content of chlorogenic acid was found in flower buds (3.3 ± 1.0 mg g−1 of DW, cv. ‘Rozela’). Whole apple fruits harvested in September were rich in chlorogenic acid and phloridzin. The statistical evaluation by Scheffe’s test confirmed the significant difference of cv. ‘Rozela’ from the other tested cultivars. In conclusion, apple tree bark, twigs, and leaves were found to be important renewable resources of bioactive phenolics, especially phloridzin and rutin. The simple availability of waste plant material can therefore be used as a rich source of phenolic compounds for cosmetics, nutraceuticals, and food supplement preparation.
Inhibitory Effect of Osthole from Cnidium monnieri (L.) Cusson on Fusarium oxysporum, a Common Fungal Pathogen of Potato
Authors: Zheng Hongli , Chen Yahan , Guo Qiuli , Wei Hong , Yue Jianying , Zhou Hongyou , Zhao Mingmin
Published: June 2021

Fusarium wilt of potato is one of the most common diseases of potato in China, and is becoming a serious threat in potato production. It has been reported that osthole from Cnidium monnieri (L.) Cusson can inhibit plant pathogens. Here, we test the anti-fungal activity of C. monnieri osthole against Fusarium oxysporum in potatoes. The results showed that at a concentration of 5 mg/mL, osthole was able to obviously inhibit mycelial growth of F. oxysporum. We found that osthole caused changes of mycelial morphology, notably hyphal swelling and darkening. Osthole significantly reduced the spore germination of Fusarium by 57.40%. In addition, osthole also inhibited the growth of other pathogens such as Fusarium moniliforme J. Sheld, Thanatephorus cucumeris Donk, and Alternaria alternata (Fr.) Keissl, but not Alternaria solani Jonesetgrout and Valsa mali Miyabe and G. Yamada. Our results suggest that osthole has considerable potential as an agent for the prevention and treatment of potato Fusarium wilt.
Alnus sibirica Compounds Exhibiting Anti-Proliferative, Apoptosis-Inducing, and GSTP1 Demethylating Effects on Prostate Cancer Cells
Authors: Seonu Seo-Yeon , Kim Min-Ji , Yin Jun , Lee Min-Won
Published: June 2021

Alnus sibirica (AS) is distributed in Korea, Japan, China, and Russia and has reported anti-oxidant, anti-inflammatory, and reducing activities on atopic dermatitis-like skin lesions, along with other beneficial health properties. In the present study, we tried to prove the cancer-preventive activity against prostate cancer. The extracted and isolated compounds, oregonin (1), hirsutenone (2), and hirsutanonol (3), which were isolated from AS, were tested for anti-proliferative activity. To do this, we used the MTT assay; NF-κB inhibitory activity, using Western blotting; apoptosis-inducing activity using flow cytometry; DNA methylation activity, using methylation-specific polymerase chain reaction in androgen-dependent (LNCaP) and androgen-independent (PC-3) prostate cancer cell lines. The compounds (1–3) showed potent anti-proliferative activity against both prostate cancer cell lines. Hirsutenone (2) exhibited the strongest NF-κB inhibitory and apoptosis-inducing activities compared with oregonin (1) and hirsutanonol (3). DNA methylation activity, which was assessed for hirsutenone (2), revealed a concentration-dependent enhancement of the unmethylated DNA content and a reduction in the methylated DNA content in both PC-3 and LNCaP cells. Overall, these findings suggest that hirsutenone (2), when isolated from AS, may be a potential agent for preventing the development or progression of prostate cancer.
Bioaerogels: Promising Nanostructured Materials in Fluid Management, Healing and Regeneration of Wounds
Authors: Bernardes Beatriz G. , Del Gaudio Pasquale , Alves Paulo , Costa Raquel , García-Gonzaléz Carlos A. , Oliveira Ana Leite
Published: June 2021

Wounds affect one’s quality of life and should be managed on a patient-specific approach, based on the particular healing phase and wound condition. During wound healing, exudate is produced as a natural response towards healing. However, excessive production can be detrimental, representing a challenge for wound management. The design and development of new healing devices and therapeutics with improved performance is a constant demand from the healthcare services. Aerogels can combine high porosity and low density with the adequate fluid interaction and drug loading capacity, to establish hemostasis and promote the healing and regeneration of exudative and chronic wounds. Bio-based aerogels, i.e., those produced from natural polymers, are particularly attractive since they encompass their intrinsic chemical properties and the physical features of their nanostructure. In this work, the emerging research on aerogels for wound treatment is reviewed for the first time. The current scenario and the opportunities provided by aerogels in the form of films, membranes and particles are identified to face current unmet demands in fluid managing and wound healing and regeneration.
Comparison of Controller-Based Locomotion Techniques for Visual Observation in Virtual Reality
Authors: Rantala Jussi , Kangas Jari , Koskinen Olli , Nukarinen Tomi , Raisamo Roope
Published: June 2021

Many virtual reality (VR) applications use teleport for locomotion. The non-continuous locomotion of teleport is suited for VR controllers and can minimize simulator sickness, but it can also reduce spatial awareness compared to continuous locomotion. Our aim was to create continuous, controller-based locomotion techniques that would support spatial awareness. We compared the new techniques, slider and grab, with teleport in a task where participants counted small visual targets in a VR environment. Task performance was assessed by asking participants to report how many visual targets they found. The results showed that slider and grab were significantly faster to use than teleport, and they did not cause significantly more simulator sickness than teleport. Moreover, the continuous techniques provided better spatial awareness than teleport.
Self-Photoluminescence of Unzipped Multi-Walled Carbon Nanotubes
Authors: Chen Mengyao , Qi Xiaohua , Zhang Wenna , Yang Na , Yang Di , Wang Yao , Zhang Lixiu , Yang Wenbin , Huang Linjun , Zhang Miaorong , Wang Shichao , Strizhak Peter , Tang Jianguo
Published: June 2021

Unzipping of carbon nanotubes (CNTs) has been widely explored to obtain new nanocarbon structures with promising properties. In this work, we report that unzipping of CNTs according to the well-established modified Hummers method produces unzipped CNTs (uCNTs) that exhibit self-photoluminescence that depends on the diameter of pristine CNTs. The uCNTs were characterized using FTIR spectroscopy, XRD, XPS, and Raman spectroscopy indicating that unzipping is accompanied by the introduction of defects and oxygen-containing functional groups. The morphology of CNTs and uCNTs was determined by TEM showing longitude unzipping of CNTs. Our study shows that increasing the diameter of pristine CNTs results in decreasing the edge etching effect and decreasing the functionality of uCNTs. Based on the UV-Vis spectra, the band gap of uCNTs was calculated using the Kubelka–Munk function. The band gap of uCNTs increased with decreasing diameter of pristine CNTs. The uCNTs exhibited photoluminescence with a good emission in the visible light region. The uCNTs with the largest band gap and the highest oxygen content had the strongest fluorescence intensity. Moreover, different metal ions produced different degrees of fluorescence quenching for uCNT-15, which verified the self-photoluminescence of uCNTs.
Preparation of a Sustainable Shape-Stabilized Phase Change Material for Thermal Energy Storage Based on Mg2+-Doped CaCO3/PEG Composites
Authors: Zahir Md. Hasan , Rahman Mohammad Mominur , Basamad Salem K. S. , Mohaisen Khaled Own , Irshad Kashif , Rahman Mohammad Mizanur , Aziz Md. Abdul , Ali Amjad , Hossain Mohammad M.
Published: June 2021

The properties of polyethylene glycol-6000 (PEG)/MgCaCO3, a low-cost shape-selective phase change material (ss-PCM), make it highly suitable for solar thermal applications. Nanosized porous MgO-doped CaCO3 with Mg molar concentrations of 5%, 10%, and 15% were synthesized using a hydrothermal technique. The prepared MgO-CaCO3 matrices were then impregnated with PEG to obtain PEG/MgCaCO3 as an ss-PCM. Samples identified as PEG-5MgCaCO3 (P-5-MCC), PEG-10MgCaCO3 (P-10-MCC), and PEG-15MgCaCO3 (P-15-MCC) were prepared and studied. Interestingly, P-10-MCC has the smallest particle size together with a good porous structure compared to the other two materials. The results of thermogravimetric analyses and differential scanning calorimetry indicate that the small particle size and porous structure facilitate the impregnation of approximately 69% of the PEG into the 10-MCC matrix. The latent heat and energy storage efficiency of PEG in the P-10-MCC sample are 152.5 J/g and 96.48%, respectively, which are significantly higher than those of comparable materials. Furthermore, in addition to the improvement of the thermal conductivity of the P-10-MCC, its supercooling is also reduced to some extent. The combined mesoporous and macro-porous structure of P-10-MCC is critical to retaining a large amount of PEG within the matrix, resulting in a high latent heat in the operating temperature range of 35–57 °C. The P-10MCC sample also demonstrates a high energy storage capacity (98.59%), high thermal energy storage/release rates, and exceptional shape-stabilized PCM properties.
PAMAM-Functionalized Cellulose Nanocrystals with Needle-Like Morphology for Effective Cancer Treatment
Authors: Sun Yanzhen , Ma Xiaoli , Jing Xiaodong , Hu Hao
Published: June 2021

Gene therapy is used to correct or compensate for diseases caused by gene defects and abnormalities. Improving the transfection efficiency and reducing the toxicity of gene carriers are the keys to gene therapy. Similar to a typical cationic gene carrier—polyethylenimine (PEI, 25 kDa)—the polyamidoamine (PAMAM) dendrimer also has a large number of amino groups. These amino groups can be complexed with nucleic acids after protonation under physiological conditions. However, the concentrated positive charge can cause undesirable cytotoxicity. Cellulose nanocrystals (CNCs) have good biocompatibility and unique needle-like morphology, and have been proven to be efficiently taken up by cells. In this article, three-dimensional spherical PMAMA dendrimers are conjugated onto the surface of CNCs to obtain a kind of needle-like cationic carrier (CNC-PAMAM). PAMAM dendrimers act as anchors to bind the plasmid DNAs (pDNA) to the surface of the CNC. The prepared CNC-based carrier showed high transfection efficiency and low toxicity. The CNC-PAMAM can effectively deliver the suicide gene to the tumor site, enabling the suicide gene/prodrug system (cytosine deaminase/5-fluorocytosine (CD/5-FC)) to play an effective anti-tumor role in vivo. This research demonstrates that the functionalization of CNCs with PAMAM dendrimers is an effective method for developing novel gene delivery systems.
Catalytic Effects of Temperature and Silicon Dioxide Nanoparticles on the Acceleration of Production from Carbonate Rocks
Authors: Salaudeen Ibraheem , Hashmet Muhammad Rehan , Pourafshary Peyman
Published: June 2021

The use of engineered water (EW) nanofluid flooding in carbonates is a new enhanced oil recovery (EOR) hybrid technique that has yet to be extensively investigated. In this research, we investigated the combined effects of EW and nanofluid flooding on oil-brine-rock interactions and recovery from carbonate reservoirs at different temperatures. EW was used as dispersant for SiO2 nanoparticles (NPs), and a series of characterisation experiments were performed to determine the optimum formulations of EW and NP for injection into the porous media. The EW reduced the contact angle and changed the rock wettability from the oil-wet condition to an intermediate state at ambient temperature. However, in the presence of NPs, the contact angle was reduced further, to very low values. When the effects of temperature were considered, the wettability changed more rapidly from a hydrophobic state to a hydrophilic one. Oil displacement was studied by injection of the optimised EW, followed by an EW-nanofluid mixture. An additional recovery of 20% of the original oil in place was achieved. The temperature effects mean that these mechanisms are catalytic, and the process involves the initiation and activation of multiple mechanisms that are not activated at lower temperatures and in each standalone technique.
Association of Graphene Silver Polymethyl Methacrylate (PMMA) with Photodynamic Therapy for Inactivation of Halitosis Responsible Bacteria in Denture Wearers
Authors: Bacali Cecilia , Carpa Rahela , Buduru Smaranda , Moldovan Mirela L. , Baldea Ioana , Constantin Annemarie , Moldovan Marioara , Prodan Doina , Dascalu (Rusu) Laura Monica , Lucaciu Ondine , Catoi Florinela , Constantiniuc Mariana , Badea Mandra
Published: June 2021

(1) Background: Poor hygiene and denture presence in the oral cavity are factors that favor bacterial accumulation, the cause of halitosis and of various oral and general diseases. Aim: This study aimed to evaluate the possibility of inactivating bacteria associated with halitosis in acrylic denture wearers using polymethyl methacrylate resin enhanced with graphene silver nanoparticles and the effect of the resin association with extra oral photodynamic therapy. (2) Methods: Graphene silver nanoparticles in 1 and 2 wt% were added to a commercial acrylic resin powder. Three study groups containing samples from the three different materials were established. The first group was not exposed to the light treatment, and the other two were exposed to red light (laser and light emitting diode) after photosensitizer placement on the disk’s surface. Samples were incubated with Porphyromonas gingivalis and Enterococcus faecalis. (3) Results: For both bacterial strains, inhibition zones were obtained, showing significant differences for the light-treated samples. (4) Conclusions: Denture resins with antibacterial properties associated with extra oral photodynamic therapy exhibited enhanced antibacterial effects. The procedure could be used as a safer and more efficient alternative technique against halitosis and oral infections in denture wearers.
Fully Inkjet-Printed Biosensors Fabricated with a Highly Stable Ink Based on Carbon Nanotubes and Enzyme-Functionalized Nanoparticles
Authors: Mass Mijal , Veiga Lionel S. , Garate Octavio , Longinotti Gloria , Moya Ana , Ramón Eloi , Villa Rosa , Ybarra Gabriel , Gabriel Gemma
Published: June 2021

Enzyme inks can be inkjet printed to fabricate enzymatic biosensors. However, inks containing enzymes present a low shelf life because enzymes in suspension rapidly lose their catalytic activity. Other major problems of printing these inks are the non-specific adsorption of enzymes onto the chamber walls and stability loss during printing as a result of thermal and/or mechanical stress. It is well known that the catalytic activity can be preserved for significantly longer periods of time and to harsher operational conditions when enzymes are immobilized onto adequate surfaces. Therefore, in this work, horseradish peroxidase was covalently immobilized onto silica nanoparticles. Then, the nanoparticles were mixed into an aqueous ink containing single walled carbon nanotubes. Electrodes printed with this specially formulated ink were characterized, and enzyme electrodes were printed. To test the performance of the enzyme electrodes, a complete amperometric hydrogen peroxide biosensor was fabricated by inkjet printing. The electrochemical response of the printed electrodes was evaluated by cyclic voltammetry in solutions containing redox species, such as hexacyanoferrate (III/II) ions or hydroquinone. The response of the enzyme electrodes was studied for the amperometric determination of hydrogen peroxide. Three months after the ink preparation, the printed enzyme electrodes were found to still exhibit similar sensitivity, demonstrating that catalytic activity is preserved in the proposed ink. Thus, enzyme electrodes can be successfully printed employing highly stable formulation using nanoparticles as carriers.