bluuebunny/crossref_metadata_2025 huggingface dataset:
PEMBERDAYAAN MASYARAKAT DALAM PEMBUATAN ALAT CUCI TANGAN OTOMATIS DAN HAND SANITIZER SEBAGAI UPAYA PENCEGAHAN VIRUS COVID-19 PADA MASA PANDEMI DI KELURAHAN BENGKONG INDAH
Authors: Yanizon Ahmad , Rofiqah Tamama
Published: April 2022

Pengabdian masyarakat ini bertujuan sebagai salah satu upaya dalam pencegahan virus covid-19 pada masa pandemic di kelurahan Bengkong Indah. Hal ini disebabkan karena pada kenyataannya, masih terdapat masyarakat yang abai terhadap peraturan pemerintah seperti tidak menggunakan masker, tidak mencuci tangan dan tidak menggunakan hand sanitizer. Hand sanitizer adalah cara praktis untuk membersihkan tangan dari kuman yang menempel, namun kebiasaan ini tidak dilakukan oleh masyarakat luas dikarenakan harga yang tinggi dan banyaknya permintaan. Selain itu masih ada sebagian masyarakat yang belum membiasakan diri mencuci tangan sebagai salah satu cara pencegahan virus Covid-19. Di tempat-tempat umum hanya terdapat beberapa kran cuci tangan, namun terkadang juga kehabisan air dan sabun. Kegiatan diawali dengan kegiatan sosialisasi dan penyuluhan berkaitan dengan pentingnya menjaga kesehatan dengan mencuci tangan dan menggunakan hand sanitizer sebagai upaya pencegahan virus covid-19. Kedua, menyiapkan bahan-bahan dan alat yang digunakan dalam pembuatan alat cuci tangan otomatis dan hand sanitizer. Ketiga, mendampingi masyarakat dalam melakukan percobaan pembuatan alat cuci tangan otomatis dan hand sanitizer. Keempat, menguji cobakan alat tersebut kepada masyarakat. Adapun hasil yang diperoleh dari kegiatan ini adalah tersedianya alat cuci tangan otomatis yang bisa digunakan masyarakat dan tersedianya hand sanitizer untuk masyarakat sebagai upaya pencegahan virus covid-19.
PELATIHAN PENGEMBANGAN KEWIRAUSAHAAN JASA KONSULTAN TEKNOLOGI INFORMASI BAGI MAHASISWA UNIVERSITAS MATHLA’UL ANWAR PANDEGLANG BANTEN
Authors: Haerani Reni , Herawati Hetty
Published: April 2022

Tujuan dari kegiatan Pengabdian Kepada Masyarakat (PKM) ini adalah untuk meningkatkan motivasi dan pemahaman berwirausaha kepada mahasiswa khususnya dalam bidang jasa konsultan Teknologi Informasi (TI). Peserta kegiatan PKM wirausaha jasa konsultan TI adalah 40 mahasiswa Fakultas Informatika dan Komputer tingkat akhir Universitas Mathla’ul Anwar Pandeglang Banten. Metode pelaksanaan pada kegiatan Pengabdian Kepada Masyarakat (PKM) ini terdiri dari lima tahap : 1) Tahap persiapan pelatihan yaitu pendataan mahasiswa yang sudah registrasi melalui link google form, kuesioner pre test dan pos test; 2) Tahap mentoring yaitu pelatihan secara personal dan kelompok, memotivasi, berbagi pengalaman ; 3) Tahap workshop yaitu pelatihan mengelola wirausaha jasa konsultan TI, pemberian keterampilan/skill teknis, bagaimana memulai wirausaha jasa konsultan TI; 4) Tahap pendampingan yaitu secara proaktif meningkatkan kualitas pelayanan pengelolaan jasa konsultan TI, pembuatan website agar menarik, pengurusan legal formal usaha, studi kasus seputar jasa konsultan TI; 5) Tahap evaluasi yaitu mengisi kuesioner melalui link google form sebagai umpan balik sejauh mana peserta memahami materi yang telah disampaikan. Berdasarkan hasil kuesioner respon peserta terhadap pelatihan diperoleh rata-rata dari aspek minat berwirausaha jasa konsultan TI 90% menjawab Ya. Dari kegiatan pelatihan ini diharapkan meningkatkan pengetahuan dan wawasan pentingnya berwirausaha dan pada akhirnya dapat menerapkan dalam lingkungan masyarakat secara menyeluruh.
OPTIMALISASI BAHAN-BAHAN PERTANIAN DALAM PEMBUATAN PESTISIDA NABATI UNTUK MENANGGULANGI HAMA PADA TANAMAN KACANG PANJANG (VIGNA SINENSIS L) DI DESA GUNUNG SELAMAT
Authors: Pramadani Delsa , Triyanto Yudi , Rizal Khairul , Walida Hilwa , Elizabeth Novilda , Harahap Dahrul Aman
Published: April 2022

Masalah yang sering dihadapi oleh petani adalah sulitnya mengatasi masalah Organisme Pengganggu Tanaman (OPT) di lahan pertanian yang masih sering mengandalkan pestisida kimia. Untuk menunjang konsep Penanggulangan Hama Terpadu (PHT) dalam rangka pengurangan penggunaan pestisida kimia perlu dicari alternatif pengendalian yang bersifat ramah lingkungan antara lain penggunaan bahan bioaktif (insektisida nabati, dan repellen), serta penggunaan perangkap berperekat. Tujuan kegiatan ini yaitu untuk merubah pola pikir petani kacang panjang dalam mengatasi masalah OPT yang cenderung menggunakan pestisida kimia. Metode dalam kegiatan ini adalah pendekatan rekayasa sosial berupa sosialisasi dan pelatihan secara langsung. Hasil kegiatan menunjukkan peningkatan kemampuan masyarakat mitra dalam pembuatan pestisida nabati. Pada kegiatan ini juga telah di ujicobakan pengunaan pestisida nabati pada pengendalian hama pada kebun contoh dan telah dilakukan analisis sempel pada tanaman kacang panjang dengan 2 petak bedengan dengan hasil memuaskan. Kegiatan pengebdian ini secara umum berjalan dengan cukup baik, namun para petani masih ada yang belum menyadari pentingnya mengendalikan hama secara nabati.
PEMANFAATAN URIN KAMBING DALAM PEMBUATAN PUPUK ORGANIK CAIR DAN IMPLEMENTASI PADA TANAMAN KACANG PANJANG (VIGNA SINENSIS L.) DI DESA GUNUNG SELAMAT
Authors: Syahputra Bintari Indrian , Rizal Khairul , Triyanto Yudi , Dalimunthe Badrul Ainy , Harahap Dahrul Aman
Published: April 2022

Masalah yang kerap dihadapi oleh petani khususnya petani kecil adalah mahalnya harga pupuk kimia, dimana pupuk merupakan faktor penentu dalam memacu tingkat produksi. Untuk menunjang kebutuhan pupuk terutama pupuk dasar, tim pengabdi melakukan tahapan pengurangan penggunaan pupuk kimia dengan menggunakan bahan alternatif, yaitu urin kambing yang dikumpulkan dari beberapa peternak kambing di sekitar desa. Tujuan umum kegiatan yaitu untuk merubah pola pikir petani kacang panjang dalam mengatasi masalah biaya pupuk yang cenderung meningkat. Sedangkan tujuan khusus kegiatan ini adalah pembuatan pupuk organik cair dari urin kambing, dan implementasinya pada tanaman kacang panjang. Metode dalam kegiatan ini yaitu pendekatan rekayasa sosial berupa sosialisasi dan pelatihan secara langsung. Hasil dari kegiatan ini yaitu berupa peningkatan pengetahuan petani dalam pembuatan pupuk organic cair dengan memanfaatkan urin kambing sebagai bahan baku utama. Kegiatan ini berjalan dengan cukup baik, namun para petani masih belum terbiasa mengunakan urin kambing untuk dijadikan pupuk organik cair.
PELATIHAN PENULISAN KARYA TULIS ILMIAH UNTUK MEMOTIVASI LITERASI MENULIS SISWA MADRASAH ALIYAH NEGERI (MAN) KARIMUN
Authors: Dewi Tisrin Maulina
Published: April 2022

Tujuan dari kegiatan Pengabdian Kepada Masyarakat (PKM) ini adalah untuk memberikan pengetahuan, pemahaman dan meningkatkan motivasi siswa tentang penulisan karya tulis ilmiah, karena selama ini banyak sekolah yang sekedar melakukan pendampingan pada saat kompetensi karya tulis ilmiah diadakan bahkan ada juga yang tidak sama sekali melakukan pendampingan. Karya tulis ilmiah yang merupakan literasi menulis siswa adalah salah satu keterampilan yang sangat penting dan harus dimiliki oleh siswa dalam menjalani pendidikan di sekolah maupun ketika nanti melanjutkan ke jenjang perguruan tinggi. Kegiatan pelatihan ini meliputi penjelasan materi tentang kaidah-kaidah penulisan karya tulis ilmiah, cara memunculkan ide kreatif kedalam sebuah tulisan serta cara mempresentasikan dalam kegiatan karya ilmiah. Kegiatan ini diikuti oleh 45 orang siswa/i dari kelas X dan XI MAN Karimun. Metode kegiatan ini ada tiga yaitu pra pelatihan, pelaksanaan pelatihan dan evaluasi pelatihan. Hasil yang diperoleh dari kegiatan ini menunjukkan bahwa pelatihan yang diberikan cukup memotivasi dan meningkatkan keterampilan siswa untuk menulis karya ilmiah.
SOSIALISASI VIRUS COVID 19 MELALUI PENDEKATAN LIVE PODCAST MEDIA SOSIAL INSTAGRAM
Authors: Sari Wanda Novita , Hasibuan Nailul Himmi
Published: April 2022

Tujuan kegiatan pengabdian masyarakat ini adalah memberikan sosialisasi mengenai virus COVID 19 serta bahaya dari virus tersebut, serta anjuran masyarakat untuk melakukan 3 M, yaitu Memakai Masker, Mencuci Tangan, Menjaga Jarak dan Menghindari kerumunan. Metode yang digunakan dalam kegiatan pelaksanaan pengabdian yang dilakukan adalah dengan melakukan sosialisasi menggunakan pendekatan live podcast media sosial Instagram. Hasil dari kegiatan pengabdian ini berupa bertambahnya pemahaman masyarakat mengenai Covid 19 yang dilihat dari antusiasme partisipan dalam memberikan pertanyaan terkait fenomena COVID 19 dan cara pencegahannya.
PELATIHAN MEDIA PEMBELAJARAN INOVATIF BERBASIS INFORMATION TECHNOLOGY (IT) DI SMP MUHAMMADIYAH 8 BATU
Authors: Lutfiana Rose Fitria , Kurniawan Moh Wahyu
Published: April 2022

Revolusi industri 4.0 membawa dampak cukup besar dalam berbagai aspek kehidupan, baik di bidang ekonomi, sosial, budaya, pendidikan dan lain sebagainya. Salah satu dampak revolusi industri dalam bidang pendidikan adalah penggunaan media pembelajaran berbasis information technology atau IT. Media pembelajaran merupakan alat penyampaian pesan dan informasi dari guru kepada siswa dalam kegiatan pembelajaran. Kegiatan pelatihan ini penting dilakukan karena relevan dengan tuntutan pembelajaran saat ini. Hasil pelatihan media pembelajaran inovatif berbasis information technology yaitu (1) 100% guru menguasai media pembelajaran inovatif berbasis information technology yang telah diajarkan; dan (2) 60% guru mengimplementasikan media pembelajaran inovatif berbasis information technology
UPAYA MEREDUKSI ANXIETY DISORDER PADA GURU MELALUI PELATIHAN EXPRESSIVE ART THERAPY
Authors: Sagita Dony Darma , Siswanto Rizki Dwi , Burhendi Feli Cianda Adrin , Andi Andi , Ramdani Ramdani
Published: April 2022

Pelatihan  ini bertujuan untuk memberikan pemahaman baru, dan melatih guru-guru BK di Kota Mataram dalam menyelesaikan permasalahan kecemasan yang mendalam pada klien. Kecemasan yang masih terjadi pada masyarakat karena berbagai kali di timpa bencana. Berbagai terapi dalam Bimbingan dan  Konseling dapat digunakan oleh guru dalam mengatasi kondisi tersebut, salah satu bentuk terapi yang bisa digunakan adalah terapi ekspressif.  Metode yang digunakan dalam pengabdian ini terdiri dari tiga tahap, 1) tahap pra pelatihan yaitu merancang model materi dan komunikasi dengan MGBK, 2) tahap pelaksanaan pelatihan dan ; 3) tahap evaluasi yaitu tanggapan peserta terhadap pelatihan.  Hasil kegiatan ini dapat dilihat dari antusiasme, keaktifan, kesungguhan dan ketertarikan peserta dalam mengikuti kegiatan ini. Evaluasi hasil menunjukkan bahwa para guru mampu memahami, merancang dan melaksanakan expressive art therapy sebagai sebuah pemahaman baru dalam penerapan pendekatan dalam Bimbingan dan Konseling dalam mereduksi kecemasan pada diri sendiri dan kliennya.
Pengembangan Media Pembelajaran Interaktif Berbasis Articulate Storyline pada Materi Komunikasi Telepon dalam Bahasa Inggris di SMK IPIEMS Surabaya
Authors: Wardani Kamelia Kusuma , Puspasari Durinta
Published: April 2022

This study aims to analyze the development process as well as the response of students to interactive media in the field of education based on Articulate Storyline on the material of Telephone Communication in English. This research method uses research and development with the ADDIE model. The subject of the research trial was a group of 20 students. X OTKP 1 SMK IPIEMS Surabaya. The data collection instrument used expert validation sheets and student response questionnaires. The data analysis technique of this research used descriptive analysis. The results of this study indicate that: 1) the process of developing interactive media for teaching and learning based on Articulate Storyline is ongoing with Telephone Communication in English class X OTKP 1 SMK IPIEMS Surabaya carried out through four steps, namely Analysis, Design, Development, and Implementation; 2) learning media is categorized as feasible based on the average validation value obtained from the three validators, which is 97%; 3) the results of student responses that obtained an average value of 92%. Therefore, it can be concluded that the interactive learning media based on Articulate Storyline on Telephone Communication in English is very feasible to be used for learning media facilities at the learning and teaching stages at SMK IPIEMS Surabaya and getting a positive response in the "very good" category by SMK IPIEMS students. Surabaya.
Potret Strategi Pengawas Sekolah dalam Meningkatkan Mutu Pembelajaran pada Masa Covid-19
Authors: Safrino Anggani , Muspawi Mohamad , Musyaiyadah Musyaiyadah
Published: April 2022

The purpose of the study was to determine the role of school supervisors in improving the quality of learning at SMP Negeri 1 Muaro Jambi during the Covid-19 pandemic. This study used qualitative methods with the type of research case study. The research subjects were the school supervisor, the principal, and five teachers of SMP Negeri 1 Muaro Jambi. The technique of collecting data is using the interview method. The data analysis technique uses data reduction, data presentation, and drawing conclusions. The results showed that the supervisor's strategies in improving the quality of learning during the Covid-19 pandemic were: 1) monitoring learning activities, 2) coaching and training, 3) and assessing teacher performance both classically and personally. Based on this research, it is recommended that school supervisors come to school more often to carry out monitoring, guidance and assessment of teachers according to the minister's instructions. The results from supervision can be evaluated and followed up to improve the teacher's ability to carry out teaching and learning during the Covid-19 pandemic.
Üniversite Öğrencilerinin Kent Ekonomisine Katkısı: Bingöl Üniversitesi Örneği
Authors: YAŞAR Mehmet Emin , GÖKOĞLAN Kadir , KÖK Meryem
Published: April 2022

Bu çalışma Bingöl Üniversitesi’nde öğrenim gören öğrencilerin harcama durumlarını belirli kriterler açısından incelemek ve il ekonomisine olan katkısını belirlemek amacıyla gerçekleştirilmiştir. Bu amaçla çalışmamızda 21 maddeden oluşan anket formu kullanılmıştır. Anket formunda yüz yüze anket yöntemi aracılığıyla basit tesadüfi örnekleme yöntemine göre seçilmiş 418 öğrencinin düşünceleri incelenmiştir. Araştırmadan elde edilen bilgiler neticesinde ankete katılan öğrencilerin genellikle devlet yurtlarında kalmayı tercih ettiği, büyük çoğunluğunun Doğu Anadolu Bölgesi’nden olduğu ve Bingöl ilinde öğrenim görme maliyetini ekonomik yönden uygun olarak gördükleri tespit edilmiştir. Ayrıca bu öğrencilerin Bingöl Üniversitesi’ni memleketlerine yakın olduğu gerekçesiyle tercih ettikleri ve Bingöl Üniversitesi’ni tavsiye etmede kararsız oldukları belirlenmiştir. Bu öğrencilerin 2019 yılında aylık ortalama 768TL gelire sahip olmalarına rağmen, aylık 1.025TL harcama yaptıkları bulgusuna ulaşılmıştır. Bingöl Üniversitesi’nin ortalama 15.713 öğrenciye sahip olduğu düşünüldüğünde öğrencilerin Bingöl şehrine yadsınamaz boyutta ekonomik katkı sağladıkları söylenebilir. Bu veriler ışığında hem üniversitede hem de il bazında öğrenci sayısını arttırmaya yönelik çabalar kentin ekonomisine önemli katkı sağlayacaktır.
HUBUNGAN BUDAYA ORGANISASI, MOTIVASI KERJA DAN LINGKUNGAN KERJA TERHADAP KEPUASAN KERJA PETUGAS KESEHATAN
Authors: Jainurakhma Janes
Published: April 2022

Some aspects of work affect the achievement of job satisfaction. Such as good work motivation, a positive work environment and a good organizational culture will foster a positive attitude in loving work and workers will feel satisfied with the work they do. The purpose of this research is to analyze the relationship of organizational culture, work motivation and work environment to the job satisfaction of health workers in Dlanggu Health Center of Mojokerto district. This research design is observational analytics with a crossectional approach. Independent variables in research are organizational culture, motivation and work environment, while dependent variables are job satisfaction. The research population is all health workers in Dlanggu Health Center 52 employees. The sample was taken with purposive sampling techniques as many as 46 respondents. The data was collected with questionnaire instruments and tested with chi square and spearman rho tests. The results showed that all values of ρ < α so that H1 is accepted means that there is a relationship between organizational culture, work motivation and the work environment to the job satisfaction of health workers in Dlanggu Health Center of Mojokerto district. Management or leadership at Dlanggu Health Center of Mojokerto Regency is expected to improve the application of organizational culture better through the attachment of the role of the organization or puskesmas in fostering morale for officers, in addition, facilities or work environments can be modified more comfortably and complete so that officers can carry out activities in providing services to patients well and officers can also feel comfortabel in working so as to spur better work motivation.
the MEDIA SOSIAL (INSTAGRAM) UNTUK PEMBELAJARAN: STUDI PADA PENDIDIKAN D3 KEBIDANAN
Authors: Mulyaningsih Erika Agung
Published: April 2022

Changes in the educational process due to the Covid-19 pandemic pose challenges for lecturers and students, the problems of online learning is a decrease in student understanding, boredom during learning and lack of motivation to be able to complete learning and assignments properly. The purpose of this research is to overcome learning problems in Basic Socio-Cultural Courses through the use of Social Media

This research is a Classroom Action Research conducted in 3 cycles, each cycle consists of 2 x 100 minutes of face-to-face and 2x 120 minutes of structured assignments, so a total of 6 x 100 minutes of face-to-face and 6 x 120 structured tasks. The subjects of this study were students in the second semester of the 2020/2021 academic year, totaling 17 people. The research was conducted from April 1 to May 17, 2021.

The results of this study have a good impact on student learning outcomes scores, learning satisfaction because it is very interesting and not boring and is able to lead students to think critically and have broad knowledge. The minimum grade score is 60; the maximum grade score is 91.8; the mean score was 86.7; and a median of 90.7.

The use of social media in learning must be relevant to the topic and Care is needed in choosing social media accounts to avoid news that cannot be justified.
Prediction models of hepatocellular carcinoma recurrence after liver transplantation: A comprehensive review
Authors: Kim Sang Jin , Kim Jong Man
Published: April 2022

Liver transplantation (LT) is one of the most effective treatments for hepatocellular carcinoma (HCC). Although LT eliminates HCC and greatly reduces recurrence, some patients experience recurrence after LT. Criteria and models for screening patients with a high probability of HCC recurrence after LT, starting with the Milan criteria, have been published. These models have changed over time, but a standard has not been established. We summarized HCC prediction models after LT by focusing on the application of radiologic, serologic, and pathologic factors and recent trends. This review will look at studies that are based on living donor LT and deceased donor LT, as well as studies that downstaging procedures have been performed preoperatively. This ultimately aims to help make decisions for evaluating the HCC state and selecting candidates for LT according to the circumstances of each transplantation center.
The Association Between Patient Characteristics and Disease Outcomes of COVID-19 in Jember, Indonesia
Authors: Mufida Diana Chusna , Lestari Putu Ayu Laksmi , Purwandhono Azham , Suswati Enny , Raharjo Angga Mardro
Published: April 2022

COVID-19 can cause serious conditions in both comorbid and elderly patients; hence, they need to be given proper follow-up treatment. This study aimed to determine the association between patient characteristics and disease outcomes of COVID-19 in Jember, Indonesia. This research is an analytic observational study with cross-sectional approach. The sample was 304 patients who were confirmed positive for COVID-19 at the COVID-19 referral hospitals in Jember. This study used medical record data analysed by using both univariate and bivariate tests. Patient characteristics related to the disease outcomes in patients who were positive for COVID-19 in Jember, are as follows: age (p = 0.000), clinical symptoms (p = 0.017), comorbidities (p = 0.000), and complications (p = 0.000). The unrelated variables were sex (p = 0.455), education level (p = 1.000), domicile (p = 1.000), occupation (p = 0.322), and smoking history (p = 0.147). Patients most at risk of developing fatal outcomes are those with complications. Patient characteristics associated with the disease outcomes in patients positive for COVID-19 were age, clinical symptoms, comorbidities, and complications. While, those that were not related to the disease outcomes in patients were sex, education level, occupation, domicile, and smoking history.
Factors Influencing the Intention of Catholic Brides and Grooms to do the Premarital HIV Test as an Attempt to Prevent HIV/AIDS in Semarang City
Authors: Alfaresedes Hillary Jeany , Shaluhiyah Zahroh , Suryoputro Antono
Published: April 2022

Semarang Health Office in 2019 recorded the age group of 25-49 years old as the most group of people with HIV. Ironically, the productive age group is the asset of the nation, also considered as the ideal group for marriage so that premarital preparation is required to result in a healthy household. On the other hand, Catholic Church has a requirement for brides and grooms to participate in Wedding Preparation Course (KPP) and is expected to increase knowledge about the household readiness as well as encourage a willingness to do the premarital HIV test as a step of wedding preparation. This study aims to analyse factors influencing the intention of catholic brides and grooms to do the premarital HIV test. The results of the study showed that the variables that influenced the intention to do premarital HIV test were knowledge (α=0.005), behavioural beliefs (α=0.004), attitude (α=0.002), subjective norms (α=0.002), and behaviour control (α=0.007). The results of multivariate analysis showed that the most influential variables were knowledge (OR=5.222), continued by subjective norms (OR=4.631), and behavioural beliefs (OR=3.825). It is suggested that KPP can collaborate with Health Office, AIDS Commission (KPA), or Non-Governmental Organization (LSM) by conducting a sharing session.
Effects of winter storms and oceanographic conditions on survival to weaning: a 37 year study of northern elephant seals on the Farallon Islands
Authors: Nur N , Berger RW , Lee DE , Warzybok PM , Jahncke J
Published: April 2022

Northern elephant seals Mirounga angustirostris were extirpated from California in the 19th century, and only in recent decades have they recolonized. A key demographic parameter underlying population viability is the survival of pups, from birth to weaning. We evaluated local factors acting directly on pup survival prior to weaning and basin-wide factors reflecting oceanic conditions which may impact maternal condition and behavior, using a 37 yr time series from 2 adjacent islands off central California: Southeast Farallon Island (SEFI) and West End Island (WEI). Mixed-effects logistic regression indicated that annual pup survival decreased with increasing frequency of extreme waves during January and February, which may inundate haulout locations when pups are present, and increased with the North Pacific Gyre Oscillation (NPGO) index, reflecting increased primary productivity. Moreover, the impact of extreme waves was manifest only for WEI, which may reflect a greater risk of inundation compared to SEFI. Annual pup survival was most highly correlated with NPGO values during the 6 mo period preceding and including arrival and the pupping period (fall and winter). Our findings suggest that favorable foraging conditions for females during the 4 mo prior to hauling out led to better maternal condition, which improved pup survival. Impacts of storm surges on pup survival is of concern since the frequency and severity of storms is expected to increase in the future. To support population recovery, we recommend studies consider demographic parameters such as recruitment of females to the Farallon breeding population and to adjacent coastal California colonies.
Eleştirel Söylem Analizinde (CDA) Sistemik İşlevsel Dil Bilim (SFL)
Authors: Fetahović Fikreta
Published: April 2022

Eleştirel söylem analizi (CDA) ve sistemik işlevsel dil bilimi (SFL) dil ve toplum arasındaki ilişkiyiinceleme konusunda ortak bir ilgiye sahiptir. CDA söylemleri analiz etmek için bir araç olaraksistemik işlevsel dil bilimi (SFL) kullanır. Başka dil modelleri olmasına rağmen, SFLꞌnın esas olarakdil kullanımına odaklanması, bilgilendirici ve sosyal işlevleri nedeniyle CDAnın özel ilgi alanınagirer. Diğer dilsel modeller de CDA için faydalıdır, ancak söz dizimsel yapıya ve dilin işlevselyönlerine daha az vurgu yaptıkları için araştırılmsı çekici değildir. Bu çalışma, sistemik işlevsel dilbilime (SFL) ve eleştirel söylem analizine (CDA) genel bir bakış sağlar ve fikirsel metafonksiyonungeçişlilik sistemi ve kişilerarası metafonksiyonun analizine odaklanır.
Evaluation of a Recirculating Aquaculture System Research Facility Designed to Address Current Knowledge Needs in Atlantic Salmon Production
Authors: Mota Vasco C. , Striberny Anja , Verstege Gerhardus C. , Difford Gareth F. , Lazado Carlo C.
Published: April 2022

A better understanding of recirculating aquaculture system (RAS) biosecurity is crucial for the sustainable and ethical production of Atlantic salmon smolt and post-smolt in these systems. This study described and evaluated the performance of a RAS facility for fish infection research with Atlantic salmon as the main animal model. Fish body weight, length, water quality, and system metrics from five independent experimental trials conducted between September 2020 and July 2021 were used to analyze the variation within and between treatments. Statistical power analysis was performed to determine the minimum number of fish required. The fish parameters variability showed that the inter-class correlation coefficient was on average low (0.1) and that the variation within tanks was larger than the variation between the tanks. The power analysis showed that 15 fish were required to be sampled per tank under these study conditions. Variation of water quality and system management metrics among the five experimental trials was higher compared to the variation within the five experimental trials. Moreover, the variation of the water quality parameters controlled by sensors was relatively low, whereas the parameters depending on biofilter maturation level and performance presented a very high variation. Water exchange rate-dependent quality parameters showed a similar variation value, i.e., nitrate and water turbidity. The established baseline for variability and performance presents an important reference for the design and realization of future experiments in RAS facilities. It is foreseen that the current research facility will develop new knowledge to improve the RAS biosecurity in the Atlantic salmon aquaculture industry.
Pathway Tools Management of Pathway/Genome Data for Microbial Communities
Authors: Karp Peter D. , Paley Suzanne , Krummenacker Markus , Kothari Anamika , Wannemuehler Michael J. , Phillips Gregory J.
Published: April 2022

The Pathway Tools (PTools) software provides a suite of capabilities for storing and analyzing integrated collections of genomic and metabolic information in the form of organism-specific Pathway/Genome Databases (PGDBs). A microbial community is represented in PTools by generating a PGDB from each metagenome-assembled genome (MAG). PTools computes a metabolic reconstruction for each organism, and predicts its operons. The properties of individual MAGs can be investigated using the many search and visualization operations within PTools. PTools also enables the user to investigate the properties of the microbial community by issuing searches across the full community, and by performing comparative operations across genome and pathway information. The software can generate a metabolic network diagram for the community, and it can overlay community omics datasets on that network diagram. PTools also provides a tool for searching for metabolic transformation routes across an organism community.
Antibacterial Cellulose Nanocrystal-Incorporated Hydrogels With Satisfactory Vascularization for Enhancing Skin Regeneration
Authors: Lu Haibin , Li Xiaoling , Zhang Mu , Xu Changpeng , Li Wenqiang , Wan Lei
Published: April 2022

Wound healing of skin defects remains a significant clinical problem due to inflammation, infection, and dysangiogenesis; especially, the promotion of microvasculature formation in healing of chronic wound or deep skin defects is critical as it supplies oxygen and nutrients to the impaired tissue, relieving uncontrolled inflammatory responses. The cellulose nanocrystals (CNCs) in the liquid crystalline phase, which facilitates cell proliferation and migration, has been shown to improve vascularization effectively. Therefore, we developed a novel injectable hydrogel based on Schiff base and coordination of catechol and Ag. The obtained hydrogels (CCS/CCHO-Ag) exhibited in situ forming properties, satisfactory mechanical performance, controlled release of Ag, antibacterial capacity, and biocompatibility. In addition, the hydrogels could also entirely cover and firmly attach wounds with irregular shapes, so as to reduce the re-injury rate. More importantly, experiments in vitro and in vivo demonstrated that CCS/CCHO-Ag hydrogels can promote neovascularization and tissue regeneration, thanks to their anti-inflammatory and antibacterial effects. In conclusion, these multifunctional hydrogels are well on the way to becoming competitive biomedical dressings, which show tremendous potential application in the field of tissue engineering.
The Role of T Cells in Ovarian Physiology and Infertility
Authors: Knapik Laura O. , Paresh Shubangi , Nabi Dalileh , Brayboy Lynae M.
Published: April 2022

Infertility affects one in six couples worldwide, with more than 48 million couples affected internationally. The prevalence of infertility is increasing which is thought to be attributed to delayed child-bearing due to socioeconomic factors. Since women are more prone to autoimmune diseases, we sought to describe the correlation between ovarian-mediated infertility and autoimmunity, and more specifically, the role of T cells in infertility. T cells prevent autoimmune diseases and allow maternal immune tolerance of the semi-allogeneic fetus during pregnancy. However, the role of T cells in ovarian physiology has yet to be fully understood.
The Role of Innate Lymphoid Cells in Cancer Development and Immunotherapy
Authors: Sugimura Rio , Wang Clement Yisai
Published: April 2022

Innate Lymphoid Cells (ILCs) are an elusive type of innate immune cell that was only discovered recently. Their tissue residency and dependency makes them a niche group of cells that bridge the adaptive and innate immune system. The nomenclature and classification of ILCs have been challenging due to their heterogeneity. The currently agreed ILC classification splits the cells into two categories including cytotoxic and helper ILCs. The tumour microenvironment is often hostile for immune cells. Remodeling the microenvironment and regulating other immune cells—achieved by ILCs-can enhance anti-tumor effects. How ILCs regulate other immune cells in the tumor microenvironment remains to be understood. Here we review current understanding of the role of ILCs in the tumor microenvironment. ILCs recruit CD8 positive T and memory T cells in PDAC, ILCs are also able to help CD108 positive B cells migrate toward tumour locations. In NSCLC, ILC3s are seen helping resident macrophages enhancing the mucus immunity to cancer cells. We then highlight the roles of cytokines and immune checkpoint pathways in ILCs and its implication in immunotherapy.
Integrative Analysis Constructs an Extracellular Matrix-Associated Gene Signature for the Prediction of Survival and Tumor Immunity in Lung Adenocarcinoma
Authors: Xiao Lingyan , Li Qian , Huang Yongbiao , Fan Zhijie , Qin Wan , Liu Bo , Yuan Xianglin
Published: April 2022

Background: Lung adenocarcinoma (LUAD) accounts for the majority of lung cancers, and the survival of patients with advanced LUAD is poor. The extracellular matrix (ECM) is a fundamental component of the tumor microenvironment (TME) that determines the oncogenesis and antitumor immunity of solid tumors. However, the prognostic value of extracellular matrix-related genes (ERGs) in LUAD remains unexplored. Therefore, this study is aimed to explore the prognostic value of ERGs in LUAD and establish a classification system to predict the survival of patients with LUAD.

Methods: LUAD samples from The Cancer Genome Atlas (TCGA) and GSE37745 were used as discovery and validation cohorts, respectively. Prognostic ERGs were identified by univariate Cox analysis and used to construct a prognostic signature by Least Absolute Shrinkage and Selection Operator (LASSO) regression analysis. The extracellular matrix-related score (ECMRS) of each patient was calculated according to the prognostic signature and used to classify patients into high- and low-risk groups. The prognostic performance of the signature was evaluated using Kaplan–Meier curves, Cox regression analyses, and ROC curves. The relationship between ECMRS and tumor immunity was determined using stepwise analyses. A nomogram based on the signature was established for the convenience of use in the clinical practice. The prognostic genes were validated in multiple databases and clinical specimens by qRT-PCR.

Results: A prognostic signature based on eight ERGs (FERMT1, CTSV, CPS1, ENTPD2, SERPINB5, ITGA8, ADAMTS8, and LYPD3) was constructed. Patients with higher ECMRS had poorer survival, lower immune scores, and higher tumor purity in both the discovery and validation cohorts. The predictive power of the signature was independent of the clinicopathological parameters, and the nomogram could also predict survival precisely.

Conclusions: We constructed an ECM-related gene signature which can be used to predict survival and tumor immunity in patients with LUAD. This signature can serve as a novel prognostic indicator and therapeutic target in LUAD.
Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3p
Authors: Liu Panpan , Qin Lihong , Liu Chang , Mi Jun , Zhang Qun , Wang Shuangshuang , Zhuang Dexuan , Xu Qiuping , Chen Wenqian , Guo Jing , Wu Xunwei
Published: April 2022

Physiological root resorption of deciduous teeth is a normal phenomenon. How the angiogenesis process is regulated to provide adequate levels of oxygen and nutrients in hypoxic conditions when the dental pulp tissue is reduced at the stage of root resorption is not fully understood. In this study, we designed hypoxic preconditioning (2%) to mimic the physiological conditions. We isolated exosomes from hypoxic-preconditioned SHED (Hypo-exos) cells and from normally cultured SHED cells (Norm-exos). We found that treatment with Hypo-exos significantly enhanced the growth, migration and tube formation of endothelial cells in vitro compared with Norm-exos. We also performed matrigel plug assays in vivo and higher expression of VEGF and higher number of lumenal structures that stained positive for CD31 were found in the Hypo-exos treated group. To understand the potential molecular mechanism responsible for the positive effects of Hypo-exos, we performed exosomal miRNA sequencing and validated that Hypo-exos transferred both let-7f-5p and miR-210-3p to promote the tube formation of endothelial cells. Further study revealed that those two miRNAs regulate angiogenesis via the let-7f-5p/AGO1/VEGF and/or miR-210-3p/ephrinA3 signal pathways. Finally, we found that the increased release of exosomes regulated by hypoxia treatment may be related to Rab27a. Taking these data together, the present study demonstrates that exosomes derived from hypoxic-preconditioned SHED cells promote angiogenesis by transferring let-7f-5p and miR-210-3p, which suggests that they can potentially be developed as a novel therapeutic approach for pro-angiogenic therapy in tissue regeneration engineering.
Uremic Toxin-Producing Bacteroides Species Prevail in the Gut Microbiota of Taiwanese CKD Patients: An Analysis Using the New Taiwan Microbiome Baseline
Authors: Shivani Subhashree , Kao Cheng-Yen , Chattopadhyay Amrita , Chen Jenn-Wei , Lai Liang-Chuan , Lin Wei-Hung , Lu Tzu-Pin , Huang I-Hsiu , Tsai Mong-Hsun , Teng Ching-Hao , Wu Jiunn-Jong , Hsieh Yi-Hsien , Wang Ming-Cheng , Chuang Eric Y.
Published: April 2022

Rationale and Objective

Gut microbiota have been targeted by alternative therapies for non-communicable diseases. We examined the gut microbiota of a healthy Taiwanese population, identified various bacterial drivers in different demographics, and compared them with dialysis patients to associate kidney disease progression with changes in gut microbiota.

Study Design

This was a cross-sectional cohort study.

Settings and Participants

Fecal samples were obtained from 119 healthy Taiwanese volunteers, and 16S rRNA sequencing was done on the V3-V4 regions to identify the bacterial enterotypes. Twenty-six samples from the above cohort were compared with fecal samples from 22 peritoneal dialysis and 16 hemodialysis patients to identify species-level bacterial biomarkers in the dysbiotic gut of chronic kidney disease (CKD) patients.

Results

Specific bacterial species were identified pertaining to different demographics such as gender, age, BMI, physical activity, and sleeping habits. Dialysis patients had a significant difference in gut microbiome composition compared to healthy controls. The most abundant genus identified in CKD patients was Bacteroides, and at the species level hemodialysis patients showed significant abundance in B. ovatus, B. caccae, B. uniformis, and peritoneal dialysis patients showed higher abundance in Blautia producta (p ≤ 0.05) than the control group. Pathways pertaining to the production of uremic toxins were enriched in CKD patients. The abundance of the bacterial species depended on the type of dialysis treatment.

Conclusion

This study characterizes the healthy gut microbiome of a Taiwanese population in terms of various demographics. In a case-control examination, the results showed the alteration in gut microbiota in CKD patients corresponding to different dialysis treatments. Also, this study identified the bacterial species abundant in CKD patients and their possible role in complicating the patients’ condition.
Profiling of the Conjunctival Bacterial Microbiota Reveals the Feasibility of Utilizing a Microbiome-Based Machine Learning Model to Differentially Diagnose Microbial Keratitis and the Core Components of the Conjunctival Bacterial Interaction Network
Authors: Ren Zhichao , Li Wenfeng , Liu Qing , Dong Yanling , Huang Yusen
Published: April 2022

Both healthy and diseased human ocular surfaces possess their own microbiota. If allowed, opportunistic pathogens within the ocular microbiota may cause microbial keratitis (MK). However, the nonpathogenic component of the ocular microbiota has been proven to undermine the performance of culture, the gold standard of the etiological diagnosis for MK. As the conjunctival bacterial microbiota generates unique alterations with various oculopathies, this study aimed to evaluate the feasibility of distinguishing MK using machine learning based on the characteristics of the conjunctival bacterial microbiome associated with various types of MK. This study also aimed to reveal which bacterial genera constitute the core of the interaction network of the conjunctival bacterial microbiome. Conjunctival swabs collected from the diseased eyes of MK patients and the randomly chosen normal eyes of healthy volunteers were subjected for high-throughput 16S rDNA sequencing. The relative content of each bacterial genus and the composition of bacterial gene functions in every sample were used to establish identification models with the random forest algorithm. Tenfold cross validation was adopted. Accuracy was 96.25% using the bacterial microbiota structure and 93.75% using the bacterial gene functional composition. Therefore, machine learning with the conjunctival bacterial microbiome characteristics might be used for differentiation of MKs as a noninvasive supplementary approach. In addition, this study found that Actinobacteria, Lactobacillus, Clostridium, Helicobacter, and Sphingomonas constitute the core of the interaction network of the conjunctival bacterial microbiome.
The Nadir Oxygen-Specific Heart Rate Response in Sleep Apnea Links With the Occurrence of Acute Myocardial Infarction
Authors: Huang Zhihua , Wu Yanpeng , Huang Kaizhuang , Chen Pingyan , Chen Jiyan , Wang Ling
Published: April 2022

Background

Little is known regarding the quantification of sleep apnea- and hypoxemia-elicited heart rate (HR) response and its prognostic significance of the cardiovascular risk. We sought to explore the impact of HR response and variability specific to obstructive sleep apnea (OSA) on the occurrence of a common cardiovascular event – acute myocardial infarction (AMI).

Methods

Consecutive patients with suspected OSA were enrolled and underwent nocturnal respiratory study and electrocardiography monitoring. The minimal oxygen saturation (minSpO2) was determined from the oxygen saturation curve under a subject-specific search window. Primary HR metrics such as maximal HR in response to minSpO2 and respiratory event-specific HR variability were computed from the synchronized recordings. Multivariate regression analyses were conducted to analyze the associations between individualized HR metrics and the occurrence of AMI.

Results

Of 2,748 patients recruited, 39% (n = 1,071) had moderate-to-severe OSA (respiratory event index, REI ≥ 15), and 11.4% (n = 313) patients had AMI. Patients with AMI experienced severe OSA, severe minSpO2, and greater HR reactions. Patients with minSpO2 <90% had an adjusted odds ratio (OR) of 1.48 [95% confidence interval (CI): 1.09–2.00, p = 0.012) for AMI. Notably, minSpO2-induced elevated mean HR response (HRmean > 73 bpm) was significantly associated with AMI (OR 1.72, 95% CI: 1.32–2.23, p < 0.001). Patients with both severe minSpO2 (<90%) and elevated HRmean carried an additive OR of 2.65 (95% CI: 1.74–4.05, p < 0.001) for the risk of AMI after adjustment for potential confounders. A large total power spectrum specific to respiratory events was correlated with an adjusted OR of 0.61 for AMI risk.

Conclusion

Patients with substantial HR reactions to OSA-induced oxygen nadir and restricted cardiac cycle shifting to respiratory events were likely at increased risk of developing AMI. Detection of nocturnal HR response to hypoxemia may help improve cardiovascular risk stratification.
Development and Comparison of Multimodal Models for Preoperative Prediction of Outcomes After Endovascular Aneurysm Repair
Authors: Wang Yonggang , Zhou Min , Ding Yong , Li Xu , Zhou Zhenyu , Shi Zhenyu , Fu Weiguo
Published: April 2022

Objective

The aim of this study was to develop and compare multimodal models for predicting outcomes after endovascular abdominal aortic aneurysm repair (EVAR) based on morphological, deep learning (DL), and radiomic features.

Methods

We retrospectively reviewed 979 patients (January 2010—December 2019) with infrarenal abdominal aortic aneurysms (AAAs) who underwent elective EVAR procedures. A total of 486 patients (January 2010–December 2015) were used for morphological feature model development and optimization. Univariable and multivariable analyses were conducted to determine significant morphological features of EVAR-related severe adverse events (SAEs) and to build a morphological feature model based on different machine learning algorithms. Subsequently, to develop the morphological feature model more easily and better compare with other modal models, 340 patients of AAA with intraluminal thrombosis (ILT) were used for automatic segmentation of ILT based on deep convolutional neural networks (DCNNs). Notably, 493 patients (January 2016–December 2019) were used for the development and comparison of multimodal models (optimized morphological feature, DL, and radiomic models). Of note, 80% of patients were classified as the training set and 20% of patients were classified as the test set. The area under the curve (AUC) was used to evaluate the predictive abilities of different modal models.

Results

The mean age of the patients was 69.9 years, the mean follow-up was 54 months, and 307 (31.4%) patients experienced SAEs. Statistical analysis revealed that short neck, angulated neck, conical neck, ILT, ILT percentage ≥51.6%, luminal calcification, double iliac sign, and common iliac artery index ≥1.255 were associated with SAEs. The morphological feature model based on the support vector machine had a better predictive performance with an AUC of 0.76, an accuracy of 0.76, and an F1 score of 0.82. Our DCNN model achieved a mean intersection over union score of more than 90.78% for the segmentation of ILT and AAA aortic lumen. The multimodal model result showed that the radiomic model based on logistics regression had better predictive performance (AUC 0.93, accuracy 0.86, and F1 score 0.91) than the optimized morphological feature model (AUC 0.62, accuracy 0.69, and F1 score 0.81) and the DL model (AUC 0.82, accuracy 0.85, and F1 score 0.89).

Conclusion

The radiomic model has better predictive performance for patient status after EVAR. The morphological feature model and DL model have their own advantages and could also be used to predict outcomes after EVAR.
Novel Compound Heterozygous PKLR Mutation Induced Pyruvate Kinase Deficiency With Persistent Pulmonary Hypertension in a Neonate: A Case Report
Authors: Lin Sha , Hua Xintian , Li Jinrong , Li Yifei
Published: April 2022

Background

Pulmonary hypertension could be associated with pyruvate kinase deficiency (PKD). There are few reported cases of PPHN as the first clinical manifestation of PKD. Herein we report a rare case of PKD in which the patient exhibited persistent pulmonary hypertension in the neonate (PPHN), and genetic testing helped to rapidly identify an potential association.

Case presentation

The patient was a newborn boy who suffered from severe dyspnea, extreme anemia, skin pallor, and hypoxemia. Repeated echocardiography indicated persistent severe pulmonary hypertension with a calculated pulmonary artery pressure of 75 mmHg, and right ventricular hypertrophy. The administration of nitric oxide significantly reduced the pulmonary artery pressure. Whole-exome sequencing revealed a compound heterozygous mutation consisting of c.707T > G and c.826_827insAGGAGCATGGGG. PolyPhen_2 and MutationTaster indicated that both the c.707T > G (probability 0.999) and c.826_827insAGGAGCATGGGG (probability 0.998) mutations were disease causing. PROVEAN protein batch analysis indicated that the associated p.L236R region was deleterious (score −4.71) and damaging (SIFT prediction 0.00), and this was also the case for p.G275_V276insEEHG (deleterious score −12.00, SIFT prediction 0.00). Substantial structural changes in the transport domain of the protein were predicted using SWISS-MODEL, and indicated that both mutations led to an unstable protein structure. Thus, a novel compound heterozygous mutation of PKLR-induced PKD with PPHN was diagnosed.

Conclusion

The current study suggests that molecular genetic screening is useful for identifying PPHN, particularly in children with metabolic disorders. In patients exhibiting unexplained hyperbilirubinemia combined with severe pulmonary hypertension, PKD might be a potential possible alternative explanation. Genetic screening is helpful for identifying genetic causes of pulmonary hypertension, especially in patients with PPHN. This report expands the mutation spectrum of the PKLR gene, and contributes to the genotype-phenotype map of PKD.