bluuebunny/crossref_metadata_2025 huggingface dataset:
Myofibril orientation as a metric for characterizing heart disease
Authors: Ma Weikang , Gong Henry , Jani Vivek , Landim-Vieira Maicon , Papadaki Maria , Pinto Jose R. , Aslam M. Imran , Cammarato Anthony , Irving Thomas
Published: September 2021

Myocyte disarray is a hallmark of many cardiac disorders. However, the relationship between alterations in the orientation of individual myofibrils and myofilaments to disease progression has been largely underexplored. This oversight has predominantly been due to a paucity of methods for objective and quantitative analysis. Here we introduce a novel, less-biased approach to quantify myofibrillar and myofilament orientation in cardiac muscle under near physiological conditions and demonstrate its superiority as compared to conventional histological assessments. Using small-angle X-ray diffraction, we first investigated changes in myofibrillar orientation at increasing sarcomere lengths in permeabilized, relaxed, wildtype mouse myocardium by assessing the angular spread of the 1,0 equatorial reflection (angle σ). At a sarcomere length (SL) of 1.9 μm, the angle σ was 0.23±0.01 rad, decreased to 0.19±0.01 rad at a SL of 2.1 μm, and further decreased to 0.15±0.01 rad at a SL of 2.3 μm (p<0.0001). Angle σ was significantly larger in R403Q, a MYH7 hypertrophic cardiomyopathy (HCM) model, porcine myocardium (0.24±0.01 rad) compared to WT myocardium (0.14±0.005 rad, p<0.0001) as well as in human heart failure tissue (0.19±0.006 rad) when compared to non-failing samples (0.17±0.007 rad, p=0.01). These data indicate that diseased myocardium suffers from greater myofibrillar disorientation compared to healthy controls. Finally, we showed that conventional, histology-based analysis of disarray can be subject to user bias and/or sampling error and lead to false positives. Our method for directly assessing myofibrillar orientation avoids the artifacts introduced by conventional histological approaches that assess myocyte orientation and only indirectly evaluate myofibrillar orientation, and provides a precise and objective metric for phenotypically characterizing myocardium. The ability to obtain excellent X-ray diffraction patterns from frozen human myocardium provides a new tool for investigating structural anomalies associated with cardiac diseases.

Statement of Significance

We introduce a precise and quantitative approach to directly measure myofibrillar and myofilament orientation in cardiac muscle under near physiological conditions as a novel tool for phenotypically characterizing striated muscle systems. We use this technique to demonstrate that myocardium from disease model organisms and failing human myocardium suffers from greater myofibrillar disorientation compared to healthy controls. We also demonstrate that excellent diffraction patterns can be obtained from frozen and thawed human myocardium. Given the ready availability of frozen human heart tissue in tissue banks, this capability opens up a large space of potential experiments relating sarcomere structure to dysfunction in cardiac disorders.
Radial-axial transport coordination enhances sugar translocation in the phloem vasculature of plants
Authors: Nakad Mazen , Domec Jean-Christophe , Sevanto Sanna , Katul Gabriel
Published: September 2021

Mass transport of photosynthates in the phloem of plants is necessary for describing plant carbon allocation, productivity, and responses to water and thermal stress. Several hypotheses about optimization of phloem structure and function and limitations of phloem transport under drought have been proposed, and tested with models and anatomical data. However, the true impact of radial water exchange of phloem conduits with their surroundings on mass transport of photosynthates has not been addressed. Here, the physics of the Munch mechanism of sugar transport is re-evaluated to include local variations in viscosity resulting from the radial water exchange in two dimensions (axial and radial). Model results show that radial water exchange pushes sucrose away from conduit walls thereby reducing wall frictional stress due to a decrease in sap viscosity and an increase in sugar concentration in the central region of the conduit. These two co-occurring effects lead to increased sugar front speed and axial mass transport across a wide range of phloem conduit lengths. Thus, sugar transport operates more efficiently than predicted by previous models that ignore these two effects. A faster front speed leads to higher phloem resiliency under drought because more sugar can be transported with a smaller pressure gradient.

The overall speed of sap increased by including a concentrationdependent viscosity in axial and radial directions.
The Fe-S cluster assembly factors NFU4 and NFU5 are primarily required for protein lipoylation in mitochondria
Authors: Przybyla-Toscano Jonathan , Maclean Andrew E. , Franceschetti Marina , Liebsch Daniela , Vignols Florence , Keech Olivier , Rouhier Nicolas , Balk Janneke
Published: September 2021

Plants have evolutionarily conserved NFU-domain proteins that are targeted to plastids or mitochondria. The ‘plastid-type’ NFU1, NFU2 and NFU3 in Arabidopsis thaliana play a role in iron-sulfur (Fe-S) cluster assembly in this organelle, whereas the type-II NFU4 and NFU5 proteins have not been subjected to mutant studies in any plant species to determine their biological role. Here we confirm that NFU4 and NFU5 are targeted to the mitochondria. The proteins are constitutively produced in all parts of the plant, suggesting a housekeeping function. Double nfu4 nfu5 knockout mutants were embryonic lethal, and depletion of the proteins led to growth arrest of young seedlings. Biochemical analyses revealed that NFU4 and NFU5 are required for lipoylation of the H proteins of the glycine decarboxylase complex and the E2 subunits of other mitochondrial dehydrogenases, with little impact on Fe-S cluster-containing respiratory complexes and aconitase. Consequently, the Gly-to-Ser ratio was increased in mutant seedlings and early growth was improved by elevated CO2. In addition, pyruvate, 2-oxoglutarate and branched-chain amino acids accumulated in the nfu4 nfu5 mutants, further supporting defects in the other three mitochondrial lipoate-dependent enzyme complexes. NFU4 and NFU5 interacted with mitochondrial lipoyl synthase (LIP1) in yeast 2-hybrid and bimolecular fluorescence complementation assays. These data indicate that NFU4 and NFU5 have a more specific function than previously thought, in providing Fe-S clusters to lipoyl synthase.

One sentence summary

A pair of evolutionarily conserved proteins involved in iron-sulfur cofactor assembly have a specific role in lipoate biosynthesis for mitochondrial dehydrogenases.
Genetic interplay between type II topoisomerase enzymes and chromosomal ccdAB toxin-antitoxin in E. coli
Authors: Kim Jay W. , Blay Vincent , Mira Portia , Barlow Miriam , Camps Manel
Published: September 2021

Fluoroquinolones are one of the most widely used class of antibiotics. They target two type II topoisomerase enzymes: gyrase and topoisomerase IV. Resistance to these drugs, which is largely caused by mutations in their target enzymes, is on the rise and becoming a serious public health risk. In this work, we analyze the sequences of 352 extraintestinal E. coli clinical isolates to gain insights into the selective pressures shaping the type II topoisomerase mutation landscape in E. coli. We identify both Quinolone Resistance-Determining Region (QRDR) and non-QRDR mutations, outline their mutation trajectories, and show that they are likely driven by different selective pressures. We confirm that ciprofloxacin resistance is specifically and strongly associated with QRDR mutations. By contrast, non-QRDR mutations are associated with the presence of the chromosomal version of ccdAB, a toxin-antitoxin operon, where the toxin CcdB is known to target gyrase. We also find that ccdAB and the evolution of QRDR mutation trajectories are partially incompatible. Finally, we identify partial deletions in CcdB and additional mutations that likely facilitate the compatibility between the presence of the ccdAB operon and QRDR mutations. These “permissive” mutations are all found in ParC (a topoisomerase IV subunit). This, and the fact that CcdB-selected mutations frequently map to topoisomerase IV, strongly suggests that this enzyme (in addition to gyrase) is likely a target for the toxin CcdB in E. coli, although an indirect effect on global supercoiling cannot be excluded. This work opens the door for the use of the presence of ccdB and of the proposed permissive mutations in the genome as genetic markers to assess the risk of quinolone resistance evolution and implies that certain strains may be genetically more refractory to evolving quinolone resistance through mutations in target enzymes.
Region-specific KCC2 rescue by rhIGF-1 and oxytocin in a mouse model of Rett syndrome
Authors: Gigliucci Valentina , Teutsch Jasper , Woodbury-Smith Marc , Luoni Mirko , Busnelli Marta , Chini Bice , Banerjee Abhishek
Published: September 2021

Rett syndrome (RTT) is characterized by dysfunction in neuronal excitation/inhibition (E/I) balance, potentially impacting seizure susceptibility via deficits in K+/Cl- cotransporter 2 (KCC2) function. Mice lacking the Methyl-CpG binding protein 2 (MeCP2) recapitulate many symptoms of RTT, and recombinant human insulin-like growth factor-1 (rhIGF-1) restores KCC2 expression and E/I balance in MeCP2 KO mice. However, clinical trial outcomes of rhIGF-1 in RTT have been variable, and increasing its therapeutic efficacy is highly desirable. To this end, the neuropeptide oxytocin (OXT) is promising, as it also critically modulates KCC2 function during early postnatal development. We measured basal KCC2 expression levels in MeCP2 KO mice and identified three key frontal brain regions showing KCC2 alterations in young adult mice but not in postnatal P10 animals. We thus hypothesized that deficits in an IGF-1/OXT signaling crosstalk modulating KCC2 may occur in RTT during postnatal development. Consistently, we detected alterations of IGF-1 receptor (IGF-1R) and OXT receptor (OXTR) levels in those brain areas. rhIGF-1 and OXT treatments in KO mice rescued KCC2 expression in a region-specific and complementary manner. These results suggest that region-selective combinatorial pharmacotherapeutic strategies could be the most effective at normalizing E/I balance in key brain regions subtending the RTT pathophysiology.
Identification of bacteriophage genome sequences with representation learning
Authors: Bai Zeheng , Zhang Yao-zhong , Miyano Satoru , Yamaguchi Rui , Fujimoto Kosuke , Uematsu Satoshi , Imoto Seiya
Published: September 2021

Motivation

Bacteriophages/Phages are the viruses that infect and replicate within bacteria and archaea, and rich in human body. To investigate the relationship between phages and microbial communities, the identification of phages from metagenome sequences is the first step. Currently, there are two main methods for identifying phages: database-based (alignment-based) methods and alignment-free methods. Database-based methods typically use a large number of sequences as references; alignment-free methods usually learn the features of the sequences with machine learning and deep learning models.

Results

We propose INHERIT which uses a deep representation learning model to integrate both database-based and alignment-free methods, combining the strengths of both. Pre-training is used as an alternative way of acquiring knowledge representations from existing databases, while the BERT-style deep learning framework retains the advantage of alignment-free methods. We compare INHERIT with four existing methods on a third-party benchmark dataset. Our experiments show that INHERIT achieves a better performance with the F1-score of 0.9932. In addition, we find that pre-training two species separately helps the non-alignment deep learning model make more accurate predictions.

Availability

The codes of INHERIT are now available in: https://github.com/Celestial-Bai/INHERIT.

Contact

yaozhong@ims.u-tokyo.ac.jp and imoto@hgc.jp

Supplementary information

Supplementary data are available at BioRxiv online.
Glycerol-driven Denitratation: Process Kinetics, Microbial Ecology, and Operational Controls
Authors: Baideme Matthew P. , Long Chenghua , Plante Luke T. , Starke Jeffrey A. , Butkus Michael A. , Chandran Kartik
Published: September 2021

Denitratation, the selective reduction of nitrate to nitrite, is a novel process when coupled with anaerobic ammonium oxidation (anammox) could achieve resource-efficient biological nitrogen removal of ammonium- and nitrate-laden waste streams. Using a fundamentally-based, first principles approach, this study optimized a stoichiometrically-limited, glycerol-driven denitratation process and characterized mechanisms supporting nitrite accumulation with results that aligned with expectations. Glycerol supported selective nitrate reduction to nitrite and near-complete nitrate conversion, indicating its viability in a denitratation system. Glycerol-supported specific rates of nitrate reduction (135.3 mg-N/g-VSS/h) were at least one order of magnitude greater than specific rates of nitrite reduction (14.9 mg-N/g-VSS/h), potentially resulting in transient nitrite accumulation and indicating glycerol’s superiority over other organic carbon sources in denitratation systems. pH and ORP inflection points in nitrogen transformation assays corresponded to maximum nitrite accumulation, indicating operational setpoints to prevent further nitrite reduction. Denitratation conditions supported enrichment of Thauera sp. as the dominant genus. Stoichiometric limitation of influent organic carbon, coupled with differential nitrate and nitrite reduction kinetics, optimized operational controls, and a distinctively enriched microbial ecology, was identified as causal in glycerol-driven denitratation.
High-level carbapenem tolerance requires antibiotic-induced outer membrane modifications
Authors: Murtha Andrew N. , Kazi Misha I. , Schargel Richard D. , Cross Trevor , Fihn Conrad , Carlson Erin E. , Boll Joseph M. , Dörr Tobias
Published: September 2021

Antibiotic tolerance is an understudied potential contributor to antibiotic treatment failure and the emergence of multidrug-resistant bacteria. The molecular mechanisms governing tolerance remain poorly understood. A prominent type of β-lactam tolerance relies on the formation of cell wall-deficient spheroplasts, which maintain structural integrity via their outer membrane (OM), an asymmetric lipid bilayer consisting of phospholipids on the inner leaflet and a lipid-linked polysaccharide (lipopolysaccharide, LPS) enriched in the outer monolayer on the cell surface. How a membrane structure like LPS, with its reliance on mere electrostatic interactions to maintain stability, is capable of countering internal turgor pressure is unknown. Here, we have uncovered a novel role for the PhoPQ two-component system in tolerance to the β-lactam antibiotic meropenem in Enterobacterales. We found that PhoPQ is induced by meropenem treatment and promotes an increase in 4-amino-4-deoxy-L-aminoarabinose [L-Ara4N] modification of lipid A, the membrane anchor of LPS. L-Ara4N modifications enhance structural integrity, and consequently tolerance to meropenem, in several Enterobacterales species. Importantly, mutational inactivation of the negative PhoPQ regulatory elementmgrB(commonly selected for during clinical therapy with the last-resort antibiotic colistin, an antimicrobial peptide [AMP]) results in dramatically enhanced tolerance, suggesting that AMPs can collaterally select for meropenem tolerance via stable overactivation of PhoPQ. Lastly, we identify histidine kinase inhibitors (including an FDA-approved drug) that inhibit PhoPQ-dependent LPS modifications and consequently potentiate meropenem to enhance killing of tolerant cells. In summary, our results suggest that PhoPQ-mediated LPS modifications play a significant role in stabilizing the OM, promoting survival when the primary integrity maintenance structure, the cell wall, is removed.

Significance

Treating an infection with an antibiotic often fails, resulting in a tremendous public health burden. One understudied likely reason for treatment failure is the development of “antibiotic tolerance”, the ability of bacteria to survive normally lethal exposure to an antibiotic. Here, we describe a molecular mechanism promoting tolerance. A bacterial stress sensor (PhoPQ) is activated in response to antibiotic (meropenem) treatment and consequently strengthens a bacterial protective “shell” to enhance survival. We also identify inhibitors of this mechanism, opening the door to developing compounds that help antibiotics work better against tolerant bacteria.
Summarizing internal dynamics boosts differential analysis and functional interpretation of super enhancers
Authors: Liu Xiang , Zhao Bo , Shaw Timothy I. , Fridley Brooke L. , Duckett Derek R. , Tan Aik-Choon , Teng Mingxiang
Published: September 2021

Super enhancers (SEs) are broad enhancer domains usually containing multiple constituent enhancers that hold elevated activities in gene regulation. Disruption in one or more constituent enhancers causes aberrant SE activities that lead to gene dysregulation in diseases. To quantify SE aberrations, differential analysis is performed to compare SE activities between cell conditions. The state-of-art strategy in estimating differential SEs relies on overall activities and neglect the changes in length and structure of SEs. Here, we propose a novel computational method to identify differential SEs by weighting the combinatorial effects of constituent-enhancer activities and locations (i.e., internal dynamics). In addition to overall activity changes, our method identified four novel classes of differential SEs with distinct enhancer structural alterations. We demonstrate that these structure alterations hold distinct regulatory impact, such as regulating different number of genes and modulating gene expression with different strengths, highlighting the differentiated regulatory roles of these unexplored SE features. When compared to the existing method, our method showed improved identification of differential SEs that were linked to better discernment of cell-type-specific SE activity and functional interpretation. We implement an R package, DASE, to facilitate the use of our method.
Incidence Rates of Pediatric Central Nervous Tumors Display No Geographical Variation
Authors: Grogan Dayton , Reddy Vamsi , Banerjee Christopher , Waller Jennifer , Johnson Theodore , Heger Ian
Published: September 2021

Background

Central nervous system (CNS) tumors affect over 4,600 children throughout the United States each year. Despite recent trends of increasing incidence of pediatric CNS tumors, the understanding of variations in their incidence between different geographical regions remains incomplete.

Method

Data used in this study was obtained from the Surveillance, Epidemiology, and End Results (SEER) Program. The SEER database and its built-in operation software was used to generate state-specific incidence data for newly diagnosed CNS tumor diagnoses in children ages 0-19 for the years 2001-2014. Results were organized by tumor type and individual states were clustered into nine geographical regions as defined by the United States Census Bureau.

Results

Statistically significant differences were found in the regional incidence of astrocytoma, primitive neuroectodermal tumor (PNET), and the category of unspecified intracranial and intraspinal neoplasms. However, the magnitude of the difference in incidence (ΔI) between specific regions was small, on the order of 0.1 to 0.6 per 100,000 population, representing a nominal 0.05-fold to 0.79-fold change in incidence (ΔI/incidence for comparator region) for astrocytoma and for the category of unspecified intracranial and intraspinal neoplasms, and a larger 3.25-fold to 3.75-fold change in incidence for PNET.

Conclusions

Differences in incidence between geographical regions for certain CNS tumor types met the bar for statistical significance. However, these differences are unlikely to be clinically meaningful due to the small effect size.
The impact of SARS-CoV-2 vaccination on Alpha and Delta variant transmission
Authors: Eyre David W , Taylor Donald , Purver Mark , Chapman David , Fowler Tom , Pouwels Koen , Walker Ann Sarah , Peto Tim EA
Published: September 2021

Background Pre-Delta, vaccination reduced SARS-CoV-2 transmission from individuals infected despite vaccination, potentially via reducing viral loads. While vaccination still lowers the risk of infection, similar viral loads in vaccinated and unvaccinated individuals infected with Delta question how much vaccination prevents transmission. Methods We performed a retrospective observational cohort study of adult contacts of SARS-CoV-2-infected adult index cases using English contact testing data. We used multivariable Poisson regression to investigate associations between transmission and index case and contact vaccination, and how these vary with Alpha and Delta variants (classified using S-gene detection/calendar trends) and time since second vaccination. Results 54,667/146,243(37.4%) PCR-tested contacts of 108,498 index cases were PCR-positive. Two doses of BNT162b2 or ChAdOx1 vaccines in Alpha index cases were independently associated with reduced PCR-positivity in contacts (aRR, adjusted rate ratio vs. unvaccinated=0.32[95%CI 0.21-0.48] and 0.48[0.30-0.78] respectively). The Delta variant attenuated vaccine-associated reductions in transmission: two BNT162b2 doses reduced Delta transmission (aRR=0.50[0.39-0.65]), more than ChAdOx1 (aRR=0.76[0.70-0.82]). Variation in Ct values (indicative of viral load) explained 7-23% of vaccine-associated transmission reductions. Transmission reductions declined over time post-second vaccination, for Delta reaching similar levels to unvaccinated individuals by 12 weeks for ChAdOx1 and attenuating substantially for BNT162b2. Protection in contacts also declined in the 3 months post-second vaccination. Conclusions Vaccination reduces transmission of Delta, but by less than the Alpha variant. The impact of vaccination decreased over time. Factors other than PCR Ct values at diagnosis are important in understanding vaccine-associated transmission reductions. Booster vaccinations may help control transmission together with preventing infections.
A virus-specific monocyte inflammatory phenotype is induced by SARS-CoV2 at the immune-epithelial interface
Authors: Leon Juliette , Michelson Daniel A. , Olejnik Judith , Chowdhary Kaitavjeet , Oh Hyung Suk , Hume Adam J. , Galván-Peña Silvia , Zhu Yangyang , Chen Felicia , Vijaykumar Brinda , Yang Liang , Crestani Elena , Yonker Lael M. , Knipe David M. , Mühlberger Elke , Benoist Christophe
Published: September 2021

Infection by SARS-CoV2 provokes a potentially fatal pneumonia with multiorgan failure, and high systemic inflammation. To gain mechanistic insight and ferret out the root of this immune dysregulation, we modeled by in vitro co-culture the interactions between infected epithelial cells and immunocytes. A strong response was induced in monocytes and B cells, with a SARS-CoV2-specific inflammatory gene cluster distinct from that seen in influenza-A or Ebola virus-infected co-cultures, and which reproduced deviations reported in blood or lung myeloid cells from COVID-19 patients. A substantial fraction of the effect could be reproduced after individual transfection of several SARS-CoV2 proteins (Spike and some non-structural proteins), mediated by soluble factors, but not via transcriptional induction. This response was greatly muted in monocytes from healthy children, perhaps a clue to the age-dependency of COVID-19. These results suggest that the inflammatory malfunction in COVID-19 is rooted in the earliest perturbations that SARS-CoV2 induces in epithelia.
Benefits and challenges to applying IPD: experiences from a Norwegian mega-project
Authors: Rodrigues Monique Rieger , Lindhard Søren Munch
Published: September 2021

Purpose

The traditional construction delivery method is challenged by low trust and collaboration issues, resulting in increased project costs. The integrated project delivery (IPD) method is developed, through a contractual agreement, to overcome these challenges by creating a common set of terms, expectations and project goals.

Design/methodology/approach

A singular construction case was followed during a four-month period. Data collection consisted of contract documents and a series of semi-structured interviews with representatives from the owner, design-group and contractors.

Findings

The IPD contract was found to have a number of positive effects; it improved project behavior (e.g. trust, collaboration and communication), increased ownership among project participants and improved buildability of the design, leading to fewer surprises and interruptions in the construction phase. The study also revealed a number of challenges including contractual and legal challenges and involving too many participants in the early phases. Moreover, co-location was identified as a particular important supporting element, to build relations and improve collaboration.

Originality/value

This research identified lessons learned from the application, as well as initial barriers and persistent barriers for implementing IPD. To improve IPD application the top three lessons were as follows: 1) the contractual documents should be adapted and signed at an early stage as this increases financial transparency, 2) cost estimates should be carried as an iterative process and project main concept be freezed at an early stage to increase understanding and minimize risks, 3) only the most important project developers should be involved in the early phases, to avoid going into detailed design issues before the main concept is completed.
Effect of unbalanced magnetic pull on the thermal characteristics of traction motor bearing
Authors: Wang Tingting , Song Dongli , Zhang Weihua , Jiang Shiqi , Wang Zhiwei
Published: September 2021

Purpose

The purpose of this paper is to analyze the unbalanced magnetic pull (UMP) of the rotor of traction motor and the influence of the UMP on thermal characteristics of traction motor bearing.

Design/methodology/approach

The unbalanced magnetic pull on the rotor with different eccentricity was calculated by Fourier series expansion method. A bearing thermal analysis finite element model considering both the vibration of high-speed train caused by track irregularity and the UMP of traction motor rotor was established. The validity of the model is verified by experimental data obtained from a service high-speed train.

Findings

The results show that thermal failure of bearing subassemblies most likely occurs at contact area between the inner ring and rollers. The UMP of rotor of traction motor has a significant effect on the temperature of the inner ring and roller of the bearing. When the eccentricity is 10%, the temperature can even be increased by about 12°C. Therefore, the UMP of rotor of traction motor must be considered in thermal analysis of traction motor bearing.

Originality/value

In the thermal analysis of the bearing of the traction motor of high-speed train, the UMP of the rotor of the traction motor is considered for the first time
Ethico-religious green supply chain management (GSCM): embedding Islamic ethics’ codes for improving environmental concerns
Authors: Islam Md Shamimul , Karia Noorliza , Taib Fauziah Md , Ara Husna , Moeinzadeh Soroush
Published: September 2021

Purpose

This study aims to propose an Ethico-Religious green supply chain management (GSCM) view grounded in Islamic teachings design to govern human beings working in the industries.

Design/methodology/approach

This study adopts a qualitative approach that used the semi-structured-interview method as a research instrument. Three experts researching various aspects of Islam were consulted to identify Islamic teachings related to green supply chain practices.

Findings

This study identifies several verses of the Holy Quran and the hadiths (a collection of traditions containing the sayings of the Prophet Muhammad) related to GSCM. It proposes these teachings as pro-environmental ethical codes.

Research limitations/implications

The proposed model has not been tested empirically. Future studies can consider an empirical test to find the possible effect of ethical codes on human behavior.

Originality/value

This study contributes to the literature in several ways. First, it presents an Ethico-Religious GSCM view that is new in the literature. Second, it extends the key premise of the natural resource-based view theory for achieving superior competitive advantage. Finally, it proposes a human governance approach useful for achieving firms’ environmental goals. This paper is helpful for managers who will find a human governance model supported by the Ethico-Religious GSCM view.
Performance evaluations and junior auditors’ attitude to audit behavior: a gender and culture comparative study
Authors: Khalil Sandra , Nehme Rabih
Published: September 2021

Purpose

The purpose of this paper is to shed light on factors leading to unethical acts committed by auditors from a cultural and gender perspectives. It investigates differences in junior auditors’ attitudes towards audit behavior when a performance evaluation (PE) is anticipated. The objective of this study is to aid academicians and audit executives in developing new models of PE and internship programs that should mitigate dysfunctional behavior.

Design/methodology/approach

A survey adapted from Big Four companies’ performance appraisal templates was administered to junior accountants who have completed their internship programs and their external audit course at accredited universities in Lebanon and the USA. Several statistical tests were conducted to analyze the relationship between the different variables.

Findings

This paper shows how PE affects junior auditors’ attitudes to dysfunctional audit behavior (DAB). From a cultural standpoint, American auditors express more negative views towards DAB than their Lebanese counterparts. This paper also demonstrates that female auditors are less inclined towards DAB than male auditors.

Originality/value

Previous studies on the topic have been mostly conducted in developed countries with a scarcity of studies examining multiple countries. This study focuses on two different cultural contexts, a developed country, the USA and an emerging country, poorly represented in the literature, Lebanon. This paper also observes variances between male and female auditors in DAB when expecting a PE. The originality of this paper stems from its concurrent examination of the impact of gender and culture on DAB by using a sample of less-experienced auditors at the end of their educational path.
Edible nanocoatings: potential food applications, challenges and safety regulations
Authors: Poonia Amrita , Mishra Alok
Published: September 2021

Purpose

This review discusses the concept of edible nanocoatings (ENCs), the biomaterials used in the coating matrices, techniques of coating development, applications, challenges and safety regulations associated with nanotechnology in food products. These ENCs are capable of imparting increased shelf life, improved appearance, better physiological qualities and bioactive potentials such as antimicrobial and antioxidant properties. ENCs can be developed using the layer-by-layer method which forms multiple alternative layers adhered together primarily by electrostatic interactions.

Design/methodology/approach

Various keywords such as edible coatings (ECs), safety aspects and nanocoatings were used to search the literature from Google Scholar, Research Gate, ScienceDirect, Springer Link, Taylor and Francis and PubMed. After searching enough literature, 113 articles and research papers were examined, which provides the updated overview of different aspects of edible nano-coatings.

Findings

Consumers today are very much aware of the food quality and its safety. They demand food products with longer shelf life, which are minimally processed with natural or no preservatives. ECs based on biopolymers is an alternative technique, which is biodegradable and can be consumed as such without posing any safety risks. The emergence of nanotechnology in food processing has provided new insights to develop ECs at the nanoscale with improved mechanical and barrier properties

Originality/value

ECs are beneficial to consumers and to the environment. ECs have generated significant attention over years as an alternative to fossil-based plastics, considering their renewable and biodegradable features
Non-revenue water reduction strategies: a systematic review
Authors: Farouk Abdelrahman M. , Rahman Rahimi A. , Romali Noor Suraya
Published: September 2021

Purpose

Sustainability involves ensuring that sufficient resources are available for current and future generations. Non-revenue water (NRW) creates a barrier to sustainability through energy and water loss. However, a comprehensive overview of NRW reduction strategies is lacking. This study reviews the existing literature to identify available strategies for reducing NRW and its components and discusses their merits.

Design/methodology/approach

A systematic literature review was conducted to identify and analyze different strategies for reducing NRW. The initial search identified 158 articles, with 41 of these deemed suitably relevant following further examination. Finally, 14 NRW reduction strategies were identified from the selected articles.

Findings

The identified NRW reduction strategies were grouped into strategies for reducing (1) apparent losses (AL), (2) real losses (RL) and (3) water losses, with the latter involving the combination of AL and RL. The strategies adopted most frequently are “prevent water leakage” and “control water pressure.” In addition, water distribution network (WDN) rehabilitation has additional benefits over other RL reduction strategies, including saving water and energy, increasing hydraulic performance and enhancing reliability. Finally, utilizing decision support systems is the only strategy capable of reducing multiple NRW categories.

Originality/value

This review provides insights into the overall NRW problem and the strategies best equipped to address it. Authorities can use these findings to develop case-specific NRW reduction action plans that save water and energy, while providing other economic benefits. In addition, NRW reduction can improve WDN reliability.
The legitimacy‐conferring capacity of constitutional courts: Evidence from a comparative survey experiment1
Authors: STERNBERG SEBASTIAN , BROUARD SYLVAIN , HÖNNIGE CHRISTOPH
Published: September 2021

Can constitutional court decisions shape public opinion on a governmental policy? Previous studies have focused on the US Supreme Court, which enjoys a high degree of public support as the major resource of power for courts. In this study, we examine the extent to which courts can influence public opinion regarding a government bill at European courts. First, we argue that the public support for courts also allows them to move public opinion on policies into the direction of their decisions. This works in both directions: they can confer legitimacy to a policy that they support, but they can also de‐legitimize a policy that they oppose. Second, we argue that this mechanism strongly depends on the amount of support that a court receives. It only has an effect for courts that possess a higher institutional legitimacy and among the group of citizens trusting a court.

We test our arguments by combining a most different systems design for France and Germany with a survey priming experiment on a school security bill. France and Germany are selected for a most different systems design as they exhibit different institutional designs as well as different levels of support for the court at the aggregate level. The survey experiment is implemented within large national election surveys, the German Internet Panel and the French National Election Study. Both experiments contain more than 2,600 respondents each. Our survey experiment primes for decision outcomes and different institutions to understand whether there are differences between an institution supporting and opposing a policy and between a court and alternative institutions.

Our findings confirm that with higher public support, courts can move the opinion of citizens to both legitimize and de‐legitimize a policy. This effect can be found at the aggregate level for a court enjoying higher public support, but also at the individual level for respondents with higher trust in the court. Interestingly, courts can even move the opinion of citizens with strong prior attitudes in the opposite direction, if these citizens highly trust the court.

These findings have implications beyond the study itself. First, they confirm that the legitimacy‐conferring effect can also be observed for European courts, not only for the US Supreme Court. Second, they show that the relevance of a mechanism identified for a single case, like the US Supreme Court, might only hold for specific conditions. As public support for courts strongly varies across countries in Europe, we also expect the impact of any mechanism relying on public support to strongly vary, as we can observe in our own analysis.
Current overview and perspectives in environmentally friendly microextractions of carbamates and dithiocarbamates
Authors: Martins Fernanda C. O. L. , Batista Alex D. , Melchert Wanessa R.
Published: September 2021

Carbamates and dithiocarbamates are two classes of pesticides widely employed in the agriculture practice to control and avoid pests and weeds, hence, the monitoring of the residue of those pesticides in different foodstuff samples is important. Thus, this review presents the classification, chemical structure, use, and toxicology of them. Moreover, it was shown the evolution of liquid‐ and solid‐phase microextractions employed in the extraction of carbamates and dithiocarbamates in water and foodstuff samples. The classification, operation mode, and application of the microextractions of liquid‐phase and solid‐phase used in their extraction were discussed and related to the analytical parameters and guidelines of green analytical chemistry.
Flualprazolam distribution in postmortem samples
Authors: Ha Helen H. , Mata Dani C.
Published: September 2021

The constant emergence of novel psychoactive substances is troubling to both public health officials and legislators. Additionally, sufficient data collection for each new compound can take months up to years. Flualprazolam, a triazolobenzodiazepine, quickly garnered attention as a sedative drug that likely expresses adverse reactions similarly to alprazolam. This study focuses on the distribution of flualprazolam in multiple common postmortem matrices. Central blood, vitreous humor, liver homogenate, brain homogenate, gastric contents, and urine samples from death investigation cases were quantitated when available. Samples were screened with liquid chromatography quadrupole time‐of‐flight with limit of detection set at 4 ng/ml and quantitated on liquid chromatography tandem mass spectrometry, with concentration range from 4 to 256 ng/ml. From August 2018 to September 2020, 24 central blood samples were quantitated for flualprazolam. Central bloods of 22 cases had concentrations above the limit of quantitation. The average flualprazolam central blood concentration was 16.3 ng/ml with a median of 9.95 ng/ml (4.24–48.0). Additional analyses for unconjugated flualprazolam were performed on at a total of 15 urine samples ( = 14.4, 4.07–36.1 ng/ml), 23 brain homogenates ( = 23.2, 3.99–69.3 ng/g), 23 liver homogenates ( = 50.7, 13.6–156 ng/g), five vitreous humor samples ( = 7.70, 4.03–12 ng/ml), and 12 gastric contents samples ( = 0.36, 0.02–2.51 mg). The cause of death for 13 of the 24 cases listed flualprazolam as a contributing factor of death.
Identifying the linguistic markers of intuition in human resource (HR) practice
Authors: Sadler‐Smith Eugene , Akstinaite Vita , Akinci Cinla
Published: September 2021

Intuitions are judgements that arise automatically and non‐consciously. Recognising when intuitive judgements are being used and whether doing so is appropriate is an important skill both for HR practitioners and managers involved in human resource (HR) processes. Because ‘intuiting’ is involuntary and unconscious it is difficult to access, monitor and control, however people can access and articulate their intuitive judgements through the spoken/written word. Identifying ‘linguistic markers’ of intuitions in spoken/written communications could help identify when/how intuition is being used in HR and improve HR practices in areas such as selection (e.g., obviating implicit/unconscious biases) and creativity (e.g., as a source of insights). This research used computerised text analysis (CTA) to identify linguistic markers of intuition based on HR practitioners' descriptions of what happens when they ‘intuit’. We outline implications of these findings for improving HR decision‐making processes and practices and their potential applications in data analytics, AI and machine learning in HR.
Secondary genepool of Australian Cajanus species contains sources of resistance to Helicoverpa armigera (Hübner)
Authors: Vanambathina Prameela , Henry Robert J. , Rachaputi Rao C. N. , Furtado Agnelo
Published: September 2021

A narrow genetic base in cultivated pigeonpea (Cajanus cajan (L.) Millsp.) limits the host plant resistance (HPR) to the devastating pest Helicoverpa armigera (Hübner). Earlier research on pod borer resistance in wild Cajanus relatives has mainly explored the tertiary genepool, making breeding for resistance difficult because of linkage drag. Previous investigations on a wide range of Australian native germplasm from the secondary and tertiary gene pools revealed resistance in the secondary gene pool, associated with total phenolic content (TPC). This study reports the application of the extreme phenotype genome‐wide association (XP‐GWAS) to associate single nucleotide polymorphisms (SNPs) with resistance traits. Mapping of 19 accessions, including 12 wild accessions to a reference pigeonpea genome have identified a total of 16,489 and 16,227 nonsynonymous SNPs for the resistance and TPC traits, respectively. These SNPs were subjected to functional analysis, and KEGG pathway analysis revealed the presence of candidate genes coding for the crucial enzymes in five defence‐related pathways that could be contributing to resistance. These pathways involve ‘Jasmonic acid synthesis’ (palmitoyl‐CoA hydrolase, phospholipase A1 and phospholipase A2), ‘insect hormone biosynthesis’ (aldehyde dehydrogenase) ‘Steroid biosynthesis’ (squalene monooxygenase, farnesyl transferase and lanosterol synthase), ‘Cutin, suberine and wax synthesis’ (O‐acyltransferase) and ‘Brassinosteroid biosynthesis’ (Det2). Analysis of SNPs associated with the TPC trait led to the identification of a divergent pathway of ‘secondary metabolite synthesis’ (flavonoid 3′ hydroxylase, l‐rhamnosyltransferase) in wild Cajanus species consistent with a high concentration of isoorientin and the possible presence of its derivative maysin; a strong insecticidal compound in leaves.
The great divergence on the Korean peninsula (1910–2020)
Authors: Kim Duol
Published: September 2021

Before the 1960s, North Korea's GDP per capita was 30%–50% higher than South Korea's due to industrialisation during the 1930s. However, the governments of the two Koreas pursued different goals in the 1960s, which resulted in a reversal. The South Korean government made economic growth its ultimate goal. They did this by self‐implementing, adjusting and instituting an export‐oriented development strategy. On the other hand, the North Korean government tried to maximise its ability to survive by sacrificing gains from economies of scale. These differences brought about remarkable differences in economic performance. The gap between the two economies has continued to grow since the income level reversal in the 1970s.
Mobilizing the Underrepresented: Electoral Systems and Gender Inequality in Political Participation
Authors: Skorge Øyvind Søraas
Published: September 2021

To study the political mobilization of underrepresented groups, this article examines the effect of electoral systems on gender equality in voting. Theoretically, I argue that replacing a plurality electoral system with proportional representation (PR) gives party elites greater incentives to mobilize women to vote in all but the most competitive districts under plurality rule. Yet, they need to tap into women's networks to succeed with such mobilizing efforts. Empirically, I isolate the causal effect of PR by studying an imposed shift from plurality to PR in Norwegian municipalities. Using a difference‐in‐differences design, I estimate that the move from plurality to PR substantially decreased gender inequality in voting. The effect is most pronounced in previously uncompetitive municipalities and where women's networks are present. This study thus demonstrates how the social environment conditions the effect of democratic institutions on the political participation of marginalized groups.
Beyond Growth Machine Politics: Understanding State Politics and National Political Mandates in China’s Urban Redevelopment
Authors: Wu Fulong , Zhang Fangzhu , Liu Yuqi
Published: September 2021

Large‐scale demolition has been ubiquitous in fast urbanising China. The politics of redevelopment is often seen as secondary, derived from and defined by local entrepreneurial governance. However, changing state politics, in particular national political mandates, has not been adequately addressed. Through examining variegated practices, this paper understands how the changing national political context affects or redefines local redevelopment projects. These cases reflect local responses to the national campaigns for rural vitalisation and “Beautiful China”, heritage preservation and “incremental regeneration” (weigaizao), and community participation in dilapidated neighbourhoods for a “harmonious society”. We find that these redevelopment projects are inherited from and associated with, but at the same time go beyond, growth machine politics. We argue that their motivations reflect a governance mode of “state entrepreneurialism” to achieve extra‐economic objectives through market instruments, and that this illustrates geopolitics, especially the role of the national state in development politics.
Letter: hepatocellular carcinoma risk in patients with non‐selective beta blockers
Authors: Huang Daniel Q. , Nguyen Mindie H.
Published: September 2021

LINKED CONTENT

This article is linked to Wijarnpreecha et al papers. To view these articles, visit https://doi.org/10.1111/apt.16590 and https://doi.org/10.1111/apt.16293
Editorial: higher concentrations of cytokine blockers are needed to obtain small bowel mucosal healing during maintenance therapy in Crohn’s disease—authors’ reply
Authors: Takenaka Kento , Kawamoto Ami , Ohtsuka Kazuo
Published: September 2021

LINKED CONTENT

This article is linked to Takenaka et al and Rakowsky et al papers. To view these articles, visit https://doi.org/10.1111/apt.16551 and https://doi.org/10.1111/apt.16571