bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring Diversity among Grapevines Varieties (Vitis vinifera L.) in Ibiza and Formentera (Balearic Islands, Spain) Using Microsatellite Markers, Ampelographic Methods and an Ethnobotanical Approach
Authors: González Raquel , Vargas Alba María , Garnatje Teresa , Vallès Joan , de Andrés María Teresa
Published: December 2023

Vitis vinifera L. has been present in Ibiza and Formentera, two islands of the Balearic Islands (Spain), since the 7th century BC. In the past few years, there have been several studies and investigations on the Balearic Islands. These have focused mainly on Mallorca and Menorca with a small representation of Ibiza and none that take into account Formentera. This research aims to contribute to the knowledge of Vitis cultivars cultivated on those islands and to investigate whether there are local cultivars still being grown. To do this, using an ethnobotanical approach, 15 persons were interviewed to gather information about local grapevines, and 36 accessions from 12 plots were characterized using ampelographic descriptors and identified using SSR markers. Relationships of the accessions studied with other cultivars were also assessed. The results show 21 different genotypes profiles, where six were new genotypes: ‘Colló de gall’, ‘Grec’, ‘Maçanet’, ‘VIEIV015-Maçanet’, ‘Morzacà’, and ‘Vermelleta’. Ten new synonyms and three homonyms have been proposed. Additionally, we suggest three new relationships for the ‘Hebén’ cultivar, one new relationship for the ‘Llora’ cultivar and one new relationship for the ‘Beba’ cultivar. These results show the first reported information for Ibiza and Formentera on Vitis.
Net Photosynthesis and Biomass Production in Stevia, Eggplant, and Cowpea Can Be Improved by Fertilization with Cyanobacteria (Limnospira maxima)
Authors: Ariza-González Anthony Ricardo , Jarma-Orozco Alfredo , Jaraba-Navas Juan de Dios , Pico-González Ana Isabel , Herazo-Cárdenas Diana Sofia , Vegliante Arrieta Daniela , Vallejo-Isaza Adriana , Pineda-Rodriguez Yirlis Yadeth , Rodriguez-Paez Luis Alfonso , Pompelli Marcelo F.
Published: December 2023

Conventional fertilizers often result in the accumulation of chemical residues in the environment with a significant threat to ecosystems, with leaching to the groundwater disrupting the delicate balance of ecosystems. To mitigate the adverse effects of chemical residues, we need new methods and the use of eco-friendly alternatives. Cyanobacteria could play a crucial role in sustainable agriculture by reducing the partial/complete use of synthetic fertilizers. This study assessed the impacts of different concentrations of Limnospira maxima extract on the physiological aspects of Vigna unguiculata, Stevia rebaudiana, and Solanum melongena. The gas exchange parameters, chlorophyll a fluorescence, and phenotypic characteristics were measured. The net photosynthesis (AN) of V. unguiculata, S. rebaudiana, and S. melongena increased by 23%, 40%, and 44%, respectively, upon the application of cyanobacteria extracts. Furthermore, the quantum yield of photosystem II showed that the extract application enhanced this response in the three species by 8.7%, 4.8%, and 11.3%, respectively. Similar results were found in the total plant biomass production with significant increases of 17%, 130%, and 80% with respect to the control. Moreover, a positive correlation was observed between AN and the majority of the evaluated parameters, which could illuminate the plant’s responses to the studied treatments. The promising potential of this cyanobacteria as a biofertilizer was accentuated.
Effects of Amino Acids on Callus Proliferation and Somatic Embryogenesis in Litchi chinensis cv. ‘Feizixiao’
Authors: Wang Guo , Liu Yaoting , Gao Zhaoyin , Li Huanling , Wang Jiabao
Published: December 2023

Amino acids (AAs) are important regulators of morphogenesis during somatic embryogenesis. This study investigated the roles of AAs in callus proliferation and embryo induction in Litchi chinensis Sonn. cv. ‘Feizixiao’ to improve the current in vitro regeneration protocols toward supporting efficient commercial litchi propagation and breeding programs. During callus proliferation, the total AAs ranged from 4627.9 μg·gFW−1 on day 3 to 7827.8 μg·gFW−1 on day 21, with an average content of 5994.0 μg·gFW−1. The total AA content continuously decreased to 3390.1 μg·gFW−1 on day 10 and slightly increased between days 10 and 20 during embryo induction, with an average content of 4849.7 μg·gFW−1. At both stages, γ-aminobutyric acid, arginine, alanine, and glutamine accounted for more than 10% of the total AAs, and methionine, tryptophan, glycine, and cystine contents were lower than 1%, except for the tyrosine content, which was also lower than 1% at the embryo induction stage. The callus proliferated on medium supplemented with a low concentration of AMO1 solution, a mixture of 21 amino acids prepared according to the average proportion of each amino acid during callus proliferation. The calli were small, yellow, and uniform, with deep Ehrlich hematoxylin staining. When cultured on medium containing a high concentration of AMO1, the callus gradually turned brown, with more dark yellow proembryos, and cell lysis and apoptosis occurred. The combination of adding 4× AMO1 solution to the proliferation medium and adding 1× AMO2 solution, which was mixed with 21 amino acids according to their average proportion during embryo induction, to the embryo induction medium had the best regeneration efficiency, with about 396 embryos and 88 regenerated plantlets per gram of callus. The results provide a basis for the rational combined application of AAs during the in vitro regeneration of litchi to achieve greater somatic embryogenesis efficiency.
The Effects of Diabetes and Being Overweight on Patients with Post-COVID-19 Syndrome
Authors: Kirbiš Simona , Sobotkiewicz Nina , Schaubach Barbara Antolinc , Završnik Jernej , Kokol Peter , Završnik Matej , Blažun Vošner Helena
Published: December 2023

In the aftermath of the COVID-19 pandemic, post-COVID-19 syndrome (PCS) remains a challenge and may continue to pose a major health problem in the future. Moreover, the influences of type 2 diabetes and being overweight on PCS remain unclear. This study aimed to assess these influences. We performed an observational study from October 2020 to July 2022, which included 466 patients (269 males and 197 females) with a median age of 65. They were hospitalized due to COVID-19 pneumonia and had persistent symptoms after 1 month of COVID-19 infection. The patients were divided into four groups according to the study objectives: patients with type 2 diabetes, overweight patients, overweight patients with type 2 diabetes, and average-weight patients without type 2 diabetes. The clinical and demographic data collected during hospitalization and regular visits to the Community Healthcare Center dr. Adolf Drolc Maribor were analyzed. Our results showed that type 2 diabetes patients had more difficult courses of treatment and longer hospitalizations. Moreover, more type 2 diabetes patients underwent rehabilitation than the other study groups. The prevailing symptoms of our patients with PCS were dyspnea and fatigue, mostly among female patients with type 2 diabetes. Our study also showed that more women with type 2 diabetes and overweight women with type 2 diabetes suffered from secondary infections. Furthermore, more overweight patients were treated in the intensive care unit than patients from the other groups. However, our study showed an interesting result: patients with type 2 diabetes had the shortest PCS durations. Type 2 diabetes and being overweight are risk factors for PCS onset and prolonged duration. Therefore, our data that revealed a shorter duration of PCS in type 2 diabetes patients than the other investigated groups was unexpected. We believe that answering the questions arising from our unexpected results will improve PCS treatment in general.
Internalized Weight Stigma and Weight Discrimination: Associations with Quality of Life and Psychosocial Impairment in a Sample Living with Food Insecurity
Authors: Cuauro Sabrina E. , Santos Natalia , Andrade Estefania , Dani Anoushka W. , Sanchious Saivone N. , Hooper Savannah C. , Becker Carolyn Black
Published: December 2023

Research suggests that experiencing weight discrimination is associated with a lower quality of life and poor psychological and physical health. However, much of the existing weight discrimination literature has neglected under-represented groups. Little is known about how the experience of weight discrimination affects quality of life and eating/weight-related psychosocial impairment in those living with food insecurity. The present study investigated the associations of weight discrimination and eating/weight-related psychosocial impairment and quality of life. We examined internalized weight stigma and several psychological indicators as potential mediators. Participants (N = 1085) who were recruited from a local food bank completed a questionnaire assessing food insecurity, weight discrimination, internalized weight stigma, eating disorder pathology, anxiety, depression, eating/weight-related psychosocial impairment, and quality of life. Overall, almost one in four participants reported experiencing weight discrimination. Our serial mediation models indicated that increased experiences of weight discrimination were associated with greater internalized weight stigma and psychopathology, which were in turn associated with lower quality of life and greater eating/weight-related psychosocial impairment. Thus, experiencing weight discrimination may negatively impact quality of life and eating/weight-related psychosocial impairment through its effect on mental health. It is imperative to address the negative effects of the widespread discrimination of people based on their weight.
Outdoor Time in Childhood: A Mixed Methods Approach to Identify Barriers and Opportunities for Intervention in a Racially and Ethnically Mixed Population
Authors: Haakenstad Magdalena K. , Butcher Maria B. , Noonan Carolyn J. , Fyfe-Johnson Amber L.
Published: December 2023

A growing body of literature suggests that outdoor time is beneficial for physical and mental health in childhood. Profound disparities exist in access to outdoor spaces (and the health benefits thereof) for children in communities of color. The objectives of this research were to: (1) identify challenges and solutions to outdoor time for children; (2) assess the importance of outdoor time for children; and (3) evaluate results stratified by race/ethnicity. Using a convergent mixed methods approach, we conducted a thematic analysis from 14 focus groups (n = 50) with outdoor educators, parents with children attending outdoor preschools, and community members with children. In addition, 49 participants completed a survey to identify challenges and solutions, perceived importance, and culturally relevant perspectives of outdoor time. The main challenges identified for outdoor time were safety concerns, inclement weather, lack of access to outdoor spaces, and parent work schedules. The primary proposed solution was integrating outdoor time into the school day. Nearly all participants, independent of racial identity, reported that outdoor time improved physical and mental health. Overall outdoor time was lower in participants from communities of color (~8 h/week) compared to their White counterparts (~10 h/week). While 50% of people of color (POC) reported that outdoor time was an important cultural value, only 18% reported that people in their respective culture spent time outside. This work contributes to accumulating knowledge that unique barriers to outdoor time exist for communities of color, and the children that live, learn, and play in these communities. Increasing outdoor time in school settings offers a potential solution to reduce identified barriers and to promote health equity in childhood.
Multiscale Analysis of Spatial Accessibility to Acute Hospitals in Carinthia, Austria
Authors: Wang Changzhen , Leitner Michael , Paulus Gernot
Published: December 2023

Health care accessibility studies are well established in the US but lacking in Austria, even though both experience high costs and have hospital care as the largest contributor to health care spending. This study aims to examine multiscale spatial accessibility to acute hospitals in Carinthia, Austria. Using the most recent data at census block and 250 meter grid levels, we refine proximity and generalized two-step floating catchment area (G2SFCA) methods while accounting for the modifiable areal unit problem (MAUP) and edge effects. For census blocks and 250 meter grids, the mean travel times to the nearest acute hospitals are 16 and 21 min, respectively, covering 58.8% and 76.2% of the population, which, however, increases to 25 and 31 min to the three nearest hospitals with similar populations. People bypassing the nearest hospital to seek hospitals at a longer distance, termed “bypass behavior”, is more influential, as 20% more of the population living in mountainous or rural areas need to travel 30 min longer. The G2SFCA method with a more pronounced distance decay results in a more decentralized polycentric structure of accessibility and identifies poorer access areas. While urban advantage is most evident in Klagenfurt and Villach, not all areas near hospitals enjoy the highest accessibility. A combination of the proximity and G2SFCA methods identifies less accessible areas. The MAUP overestimates accessibility at a coarse level and in less populous areas. Edge effects occur at the border when using proximity only, but they are more sensitive when considering bypass behavior or a weak distance decay effect. This study contributes to our understanding of acute hospitals’ accessibility in Carinthia and highlights the need to improve low-accessible areas in addition to universal health coverage. Cautions need to be exercised when using different geographic units or considering edge effects for health care planning and management.
Acid-Sensing Ion Channels’ Immunoreactivity in Nerve Profiles and Glomus Cells of the Human Carotid Body
Authors: Martínez-Barbero Graciela , García-Mesa Yolanda , Cobo Ramón , Cuendias Patricia , Martín-Biedma Benjamín , García-Suárez Olivia , Feito Jorge , Cobo Teresa , Vega José A.
Published: December 2023

The carotid body is a major peripheral chemoreceptor that senses changes in arterial blood oxygen, carbon dioxide, and pH, which is important for the regulation of breathing and cardiovascular function. The mechanisms by which the carotid body senses O2 and CO2 are well known; conversely, the mechanisms by which it senses pH variations are almost unknown. Here, we used immunohistochemistry to investigate how the human carotid body contributes to the detection of acidosis, analyzing whether it expresses acid-sensing ion channels (ASICs) and determining whether these channels are in the chemosensory glomic cells or in the afferent nerves. In ASIC1, ASIC2, and ASIC3, and to a much lesser extent ASIC4, immunoreactivity was detected in subpopulations of type I glomus cells, as well as in the nerves of the carotid body. In addition, immunoreactivity was found for all ASIC subunits in the neurons of the petrosal and superior cervical sympathetic ganglia, where afferent and efferent neurons are located, respectively, innervating the carotid body. This study reports for the first time the occurrence of ASIC proteins in the human carotid body, demonstrating that they are present in glomus chemosensory cells (ASIC1 < ASIC2 > ASIC3 > ASIC4) and nerves, presumably in both the afferent and efferent neurons supplying the organ. These results suggest that the detection of acidosis by the carotid body can be mediated via the ASIC ion channels present in the type I glomus cells or directly via sensory nerve fibers.
Molecular Dynamics and Near-Tg Phenomena of Cyclic Thioethers
Authors: Hellwig Hubert , Nowok Andrzej , Peksa Paulina , Dulski Mateusz , Musioł Robert , Pawlus Sebastian , Kuś Piotr
Published: December 2023

This article presents the synthesis and molecular dynamics investigation of three novel cyclic thioethers: 2,3-(4′-methylbenzo)-1,4,7,10-tetrathiacyclododeca-2-ene (compound 1), 2,3,14,15-bis(4′,4″(5″)-methylbenzo)-1,4,7,10,13,16,19,22,25-octathiacyclotetracosa-2,14-diene (compound 2), and 2,3,8,9-bis(4′,4″(5″)-methylbenzo)-1,4,7,10-tetrathiacyclododeca-2,8-diene (compound 3). The compounds exhibit relatively high glass transition temperatures (Tg), which range between 254 and 283 K. This characteristic positions them within the so-far limited category of crown-like glass-formers. We demonstrate that cyclic thioethers may span both the realms of ordinary and sizeable molecular glass-formers, each featuring distinct physical properties. Furthermore, we show that the Tg follows a sublinear power law as a function of the molar mass within this class of compounds. We also reveal multiple dielectric relaxation processes of the novel cyclic thioethers. Above the Tg, their dielectric loss spectra are dominated by a structural relaxation, which originates from the cooperative reorientation of entire molecules and exhibits an excess wing on its high-frequency slope. This feature has been attributed to the Johari–Goldstein (JG) process. Each investigated compound exhibits also at least one intramolecular secondary non-JG relaxation stemming from conformational changes. Their activation energies range from approximately 19 kJ/mol to roughly 40 kJ/mol. Finally, we analyze the high-pressure molecular dynamics of compound 1, revealing a pressure-induced increase in its Tg with a dTg/dp coefficient equal to 197 ± 8 K/GPa.
TMT-Based Proteomics Analysis Revealed the Protein Changes in Perirenal Fat from Obese Rabbits
Authors: Jiang Genglong , Shao Jiahao , Tang Tao , Wang Meigui , Wang Jie , Jia Xianbo , Lai Songjia
Published: December 2023

Obesity has become increasingly prevalent in recent years, and there is a need for a deeper understanding of the complex pathogenesis underlying the obesity condition. Therefore, the objective of this study was to investigate how a high-fat diet (HFD) affects protein expression in a female-rabbit model compared to a standard normal-diet group (SND), to gain comprehensive insights into the molecular mechanisms involved in obesity. To achieve this objective, a tandem mass tag (TMT)-based quantitative proteomics analysis was conducted to examine the molecular changes occurring in the white adipose tissue (WAT) from the HFD and SND groups. The sequencing results identified a total of 4215 proteins, among which 151 proteins exhibited significant differential expression. Specifically, there were 85 upregulated proteins and 66 downregulated proteins in the HFD group compared to the SND group. Further analysis of these differentially expressed proteins (DEPs) revealed their involvement in crucial biological processes, including energy metabolism, hormonal regulation, and inflammatory response. In conclusion, this study sheds light on the impact of HFD on protein expression in a female-rabbit model, providing new insights into the molecular mechanisms underlying obesity and the associated metabolic disorders.
Gypsum-Related Impact on Antibiotic-Loaded Composite Based on Highly Porous Hydroxyapatite—Advantages and Disadvantages
Authors: Zalewska Justyna , Vivcharenko Vladyslav , Belcarz Anna
Published: December 2023

Highly porous hydroxyapatite is sometimes considered toxic and useless as a biomaterial for bone tissue regeneration because of the high adsorption of calcium and phosphate ions from cell culture media. This negatively affects the osteoblast’s growth in such ion-deprived media and suggests “false cytotoxicity” of tested hydroxyapatite. In our recent study, we showed that a small addition of calcium sulfate dihydrate (CSD) may compensate for this adsorption without a negative effect on other properties of hydroxyapatite-based biomaterials. This study was designed to verify whether such CSD-supplemented biomaterials may serve as antibiotic carriers. FTIR, roughness, mechanical strength analysis, drug release, hemocompatibility, cytotoxicity against human osteoblasts, and antibacterial activity were evaluated to characterize tested biomaterials. The results showed that the addition of 1.75% gypsum and gentamicin caused short-term calcium ion compensation in media incubated with the composite. The combination of both additives also increased antibacterial activity against bacteria representative of bone infections without affecting osteoblast proliferation, hemocompatibility, and mechanical parameters. Thus, gypsum and antibiotic supplementation may provide advanced functionality for bone-regeneration materials based on hydroxyapatite of a high surface area and increasingly high Ca2+ sorption capacity.
Does Background Matter? A Comparative Characterization of Mouse Models of Autosomal Retinitis Pigmentosa rd1 and Pde6b-KO
Authors: Chirinskaite Angelina V. , Rotov Alexander Yu. , Ermolaeva Mariia E. , Tkachenko Lyubov A. , Vaganova Anastasia N. , Danilov Lavrentii G. , Fedoseeva Ksenia N. , Kostin Nicolay A. , Sopova Julia V. , Firsov Michael L. , Leonova Elena I.
Published: December 2023

Many retinal degenerative diseases result in vision impairment or permanent blindness due to photoreceptor loss or dysfunction. It has been observed that Pde6brd1 mice (rd1), which carry a spontaneous nonsense mutation in the pde6b gene, have a strong phenotypic similarity to patients suffering from autosomal recessive retinitis pigmentosa. In this study, we present a novel mouse model of retinitis pigmentosa generated through pde6b gene knockout using CRISPR/Cas9 technology. We compare this Pde6b-KO mouse model to the rd1 mouse model to gain insights into the progression of retinal degeneration. The functional assessment of the mouse retina and the tracking of degeneration dynamics were performed using electrophysiological methods, while retinal morphology was analyzed through histology techniques. Interestingly, the Pde6b-KO mouse model demonstrated a higher amplitude of photoresponse than the rd1 model of the same age. At postnatal day 12, the thickness of the photoreceptor layer in both mouse models did not significantly differ from that of control animals; however, by day 15, a substantial reduction was observed. Notably, the decline in the number of photoreceptors in the rd1 model occurred at a significantly faster rate. These findings suggest that the C3H background may play a significant role in the early stages of retinal degeneration.
MicroRNA-152-3p and MicroRNA-196a-5p Are Downregulated When Müller Cells Are Promoted by Components of the Internal Limiting Membrane: Implications for Macular Hole Healing
Authors: Chou Hung-Da , Shiah Shine-Gwo , Chuang Lan-Hsin , Wu Wei-Chi , Hwang Yih-Shiou , Chen Kuan-Jen , Kang Eugene Yu-Chuan , Yeung Ling , Nien Chung-Yi , Lai Chi-Chun
Published: December 2023

Müller cells play a critical role in the closure of macular holes, and their proliferation and migration are facilitated by the internal limiting membrane (ILM). Despite the importance of this process, the underlying molecular mechanism remains underexplored. This study investigated the effects of ILM components on the microRNA (miRNA) profile of Müller cells. Rat Müller cells (rMC-1) were cultured with a culture insert and varying concentrations of ILM component coatings, namely, collagen IV, laminin, and fibronectin, and cell migration was assessed by measuring cell-free areas in successive photographs following insert removal. MiRNAs were then extracted from these cells and analyzed. Mimics and inhibitors of miRNA candidates were transfected into Müller cells, and a cell migration assay and additional cell viability assays were performed. The results revealed that the ILM components promoted Müller cell migration (p < 0.01). Among the miRNA candidates, miR-194-3p was upregulated, whereas miR-125b-1-3p, miR-132-3p, miR-146b-5p, miR-152-3p, miR-196a-5p, miR-542-5p, miR-871-3p, miR-1839-5p, and miR-3573-3p were significantly downregulated (p < 0.05; fold change > 1.5). Moreover, miR-152-3p and miR-196a-5p reduced cell migration (p < 0.05) and proliferation (p < 0.001), and their suppressive effects were reversed by their respective inhibitors. In conclusion, miRNAs were regulated in ILM component-activated Müller cells, with miR-152-3p and miR-196a-5p regulating Müller cell migration and proliferation. These results serve as a basis for understanding the molecular healing process of macular holes and identifying potential new target genes in future research.
Dielectric Spectroscopy Studies of Conformational Relaxation Dynamics in Molecular Glass-Forming Liquids
Authors: Romanini Michela , Macovez Roberto , Valenti Sofia , Noor Wahi , Tamarit Josep Lluís
Published: December 2023

We review experimental results obtained with broadband dielectric spectroscopy concerning the relaxation times and activation energies of intramolecular conformational relaxation processes in small-molecule glass-formers. Such processes are due to the interconversion between different conformers of relatively flexible molecules, and generally involve conformational changes of flexible chain or ring moieties, or else the rigid rotation of planar groups, such as conjugated phenyl rings. Comparative analysis of molecules possessing the same (type of) functional group is carried out in order to test the possibility of assigning the dynamic conformational isomerism of given families of organic compounds to the motion of specific molecular subunits. These range from terminal halomethyl and acetyl/acetoxy groups to both rigid and flexible ring structures, such as the planar halobenzene cycles or the buckled saccharide and diazepine rings. A short section on polyesters provides a generalisation of these findings to synthetic macromolecules.
The Role of Cellular Senescence in Cyclophosphamide-Induced Primary Ovarian Insufficiency
Authors: Xu Zixin , Takahashi Nozomi , Harada Miyuki , Kunitomi Chisato , Kusamoto Akari , Koike Hiroshi , Tanaka Tsurugi , Sakaguchi Nanoka , Urata Yoko , Wada-Hiraike Osamu , Hirota Yasushi , Osuga Yutaka
Published: December 2023

Young female cancer patients can develop chemotherapy-induced primary ovarian insufficiency (POI). Cyclophosphamide (Cy) is one of the most widely used chemotherapies and has the highest risk of damaging the ovaries. Recent studies elucidated the pivotal roles of cellular senescence, which is characterized by permanent cell growth arrest, in the pathologies of various diseases. Moreover, several promising senolytics, including dasatinib and quercetin (DQ), which remove senescent cells, are being developed. In the present study, we investigated whether cellular senescence is involved in Cy-induced POI and whether DQ treatment rescues Cy-induced ovarian damage. Expression of the cellular senescence markers p16, p21, p53, and γH2AX was upregulated in granulosa cells of POI mice and in human granulosa cells treated with Cy, which was abrogated by DQ treatment. The administration of Cy decreased the numbers of primordial and primary follicles, with a concomitant increase in the ratio of growing to dormant follicles, which was partially rescued by DQ. Moreover, DQ treatment significantly improved the response to ovulation induction and fertility in POI mice by extending reproductive life. Thus, cellular senescence plays critical roles in Cy-induced POI, and targeting senescent cells with senolytics, such as DQ, might be a promising strategy to protect against Cy-induced ovarian damage.
The Recycling of Substandard Rocket Fuel N,N-Dimethylhydrazine via the Involvement of Its Hydrazones Derived from Glyoxal, Acrolein, Metacrolein, Crotonaldehyde, and Formaldehyde in Organic Synthesis
Authors: Ivanova Elizaveta , Osipova Margarita , Vasilieva Tatyana , Eremkin Alexey , Markova Svetlana , Zazhivihina Ekaterina , Smirnova Svetlana , Mitrasov Yurii , Nasakin Oleg
Published: December 2023

“Heptil” (unsymmetrical dimethylhydrazine—UDMH) is extensively employed worldwide as a propellant for rocket engines. However, UDMH constantly loses its properties as a result of its continuous and uncontrolled absorption of moisture, which cannot be rectified. This situation threatens its long-term usability. UDMH is an exceedingly toxic compound (Hazard Class 1), which complicates its transportation and disposal. Incineration is currently the only method used for its disposal, but this process generates oxidation by-products that are even more toxic than the original UDMH. A more benign approach involves its immediate reaction with a formalin solution to form 1,1–dimethyl-2-methylene hydrazone (MDH), which is significantly less toxic by an order of magnitude. MDH can then be polymerized under acidic conditions, and the resulting product can be burned, yielding substantial amounts of nitrogen oxides. This review seeks to shift the focus of MDH from incineration towards its application in the synthesis of relatively non-toxic and readily available analogs of various pharmaceutical substances. We aim to bring the attention of the international chemical community to the distinctive properties of MDH, as well as other hydrazones (such as glyoxal, acrolein, crotonal, and meta-crolyl), wherein each structural fragment can initiate unique transformations that have potential applications in molecular design, pharmaceutical research, and medicinal chemistry.
Exploring the Role of the Gut and Intratumoral Microbiomes in Tumor Progression and Metastasis
Authors: Sevcikova Aneta , Mladosievicova Beata , Mego Michal , Ciernikova Sona
Published: December 2023

Cancer cell dissemination involves invasion, migration, resistance to stressors in the circulation, extravasation, colonization, and other functions responsible for macroscopic metastases. By enhancing invasiveness, motility, and intravasation, the epithelial-to-mesenchymal transition (EMT) process promotes the generation of circulating tumor cells and their collective migration. Preclinical and clinical studies have documented intensive crosstalk between the gut microbiome, host organism, and immune system. According to the findings, polymorphic microbes might play diverse roles in tumorigenesis, cancer progression, and therapy response. Microbial imbalances and changes in the levels of bacterial metabolites and toxins promote cancer progression via EMT and angiogenesis. In contrast, a favorable microbial composition, together with microbiota-derived metabolites, such as short-chain fatty acids (SCFAs), can attenuate the processes of tumor initiation, disease progression, and the formation of distant metastases. In this review, we highlight the role of the intratumoral and gut microbiomes in cancer cell invasion, migration, and metastatic ability and outline the potential options for microbiota modulation. As shown in murine models, probiotics inhibited tumor development, reduced tumor volume, and suppressed angiogenesis and metastasis. Moreover, modulation of an unfavorable microbiome might improve efficacy and reduce treatment-related toxicities, bringing clinical benefit to patients with metastatic cancer.
Interaction of Phytohormones and External Environmental Factors in the Regulation of the Bud Dormancy in Woody Plants
Authors: Chen Zhaoyu , Chen Yadi , Shi Lanxi , Wang Li , Li Weixing
Published: December 2023

Bud dormancy and release are essential phenomena that greatly assist in adapting to adverse growing conditions and promoting the holistic growth and development of perennial plants. The dormancy and release process of buds in temperate perennial trees involves complex interactions between physiological and biochemical processes influenced by various environmental factors, representing a meticulously orchestrated life cycle. In this review, we summarize the role of phytohormones and their crosstalk in the establishment and release of bud dormancy. External environmental factors, such as light and temperature, play a crucial role in regulating bud germination. We also highlight the mechanisms of how light and temperature are involved in the regulation of bud dormancy by modulating phytohormones. Moreover, the role of nutrient factors, including sugar, in regulating bud dormancy is also discussed. This review provides a foundation for enhancing our understanding of plant growth and development patterns, fostering agricultural production, and exploring plant adaptive responses to adversity.
Optimal Hiding Strategy for Transshipments under Trade Embargoes
Authors: Fantozzi Paolo , Naldi Maurizio
Published: December 2023

Trade embargoes, often imposed for political, economic, or security reasons, have long been a tool of international diplomacy. Transshipments may be employed as a strategic mechanism by nations and organizations to circumvent trade embargoes. Transshipment involves rerouting goods through intermediary ports or countries to obscure their origin, destination, or the parties involved. This practice may be subject to investigation, which could lead to exposing the entities employing it. Strategic management of transshipments has to be devised by those entities (the attackers) battling against the transshipment detection mechanisms adopted by the embargo-setters (the defenders). In this paper, we consider an entity exploiting transshipments through several intermediaries. We derive an optimal strategy for that entity wishing to minimize the probability of being exposed. Our strategy provides the optimal number of intermediaries and the optimal distribution of goods among those intermediaries.
Cranial MRI beyond the Neonatal Period and Neurodevelopmental Outcomes in Neonatal Encephalopathy Due to Perinatal Asphyxia: A Systematic Review
Authors: Parmentier Corline E. J. , Kropman Tobias , Groenendaal Floris , Lequin Maarten H. , de Vries Linda S. , Benders Manon J. N. L. , Alderliesten Thomas
Published: December 2023

Background: Magnetic resonance imaging (MRI) including diffusion-weighted imaging within seven days after birth is widely used to obtain prognostic information in neonatal encephalopathy (NE) following perinatal asphyxia. Later MRI could be useful for infants without a neonatal MRI or in the case of clinical concerns during follow-up. Therefore, this review evaluates the association between cranial MRI beyond the neonatal period and neurodevelopmental outcomes following NE. Methods: A systematic literature search was performed using PubMed and Embase on cranial MRI between 2 and 24 months after birth and neurodevelopmental outcomes following NE due to perinatal asphyxia. Two independent researchers performed the study selection and risk of bias analysis. Results were separately described for MRI before and after 18 months. Results: Twelve studies were included (high-quality n = 2, moderate-quality n = 6, low-quality n = 4). All reported on MRI at 2–18 months: seven studies demonstrated a significant association between the pattern and/or severity of injury and overall neurodevelopmental outcomes and three showed a significant association with motor outcome. There were insufficient data on non-motor outcomes and the association between MRI at 18–24 months and neurodevelopmental outcomes. Conclusions: Cranial MRI performed between 2 and 18 months after birth is associated with neurodevelopmental outcomes in NE following perinatal asphyxia. However, more data on the association with non-motor outcomes are needed.
To Test or Not to Test: Routine Thrombophilia Diagnostic Screening of Women with Reproductive Failures
Authors: Wysocka Urszula , Sałacińska Kinga , Pinkier Iwona , Kępczyński Łukasz , Ałaszewski Wojciech , Dudarewicz Lech , Gach Agnieszka
Published: December 2023

Background: Recurrent reproductive failure is a global health issue affecting a significant number of women. Thrombophilias have been implicated as a possible cause. Inherited thrombophilias include a single nucleotide variant on factor V Leiden and prothrombin. Objective: The aim of this study was to evaluate the association between the following single nucleotide variants: factor V Leiden (c.1601G>A), the prothrombin gene (c.*97G>A) and the reproductive failure in the Polish population. Methods: The study was conducted in a group of 545 patients with recurrent pregnancy loss, RPL (≥2 miscarriages), and in a group of 641 patients with infertility. The distribution of genotypes for the selected variants were determined by RFLP-PCR and by the real-time PCR method. Results: A variant of the F5 gene was found in 5.14% of patients with RPL and in 6.08% of infertile women. A variant of the F2 gene was identified in 0.73% of patients with RPL and in 2.03% of women with infertility. The frequency in the study groups did not differ from that in the general population. No association between the studied variants of the F5 gene or the F2 gene and the predisposition to reproductive wastage was found. Conclusions: Recommendations for routine thrombophilia testing in women with recurrent miscarriages should be revisited. The decision regarding testing should be made individually depending on additional factors indicating an increased risk of venous thromboembolism.
Resection of a Solitary Right Ventricular Metastasis in Oligorecurrent Hepatocellular Carcinoma
Authors: Ergi Defne Gunes , Klarich Kyle W. , Dearani Joseph A. , Crestanello Juan A. , Maleszewski Joseph J. , Morris Jonathan M. , Young Phillip M. , Callaghan Cameron M. , Pitot Henry C. , Arghami Arman
Published: December 2023

Hepatocellular carcinoma (HCC), constituting the predominant manifestation of liver cancer, stands as a formidable medical challenge. The prognosis subsequent to surgical intervention, particularly for individuals presenting with a solitary tumor, relies heavily on the degree of invasiveness. The decision-making process surrounding therapeutic modalities in such cases assumes paramount importance. This case report illuminates a rather unusual clinical scenario. Here, we encounter a patient who, following a disease-free interval, manifested an atypical presentation of HCC, specifically, a solitary cardiac metastasis. The temporal interval of remission adds an additional layer of complexity to the case. Through a multidisciplinary planning process, the decision was made for surgical removal of the metastatic tumor.
Relationship between Anterior Cruciate Ligament Injury and Subtalar Pronation in Female Basketball Players: Case-Control Study
Authors: Carabasa García Laura , Lorca-Gutiérrez Rubén , Vicente-Mampel Juan , Part-Ferrer Roser , Fernández-Ehrling Nadia , Ferrer-Torregrosa Javier
Published: December 2023

Anterior cruciate ligament (ACL) injuries are a common issue in basketball. Several studies point to subtalar pronation as a relevant risk factor for these injuries, despite their multiarticular and multiplanar nature. This study evaluated the correlation between subtalar pronation and ACL injuries in female basketball players. A total of 30 players were recruited and divided into two groups: 15 with previous ACL injury and 15 without injury. The navicular drop test (NDT) and drop vertical jump test were applied to quantify parameters such as navicular drop, calcaneal eversion, ankle dorsiflexion, knee flexion, and dynamic valgus. The results showed significantly higher NDT values (6.93 ± 1.64 mm vs. 5.41 ± 1.96 mm, p = 0.029) and maximum calcaneal eversion angle (10.94 ± 3.22° vs. 5.30 ± 3.33°, p < 0.001) in the injured group. There were also significant differences in maximum dynamic valgus (152.73 ± 15.00° vs. 165.26 ± 5.628°, p = 0.005) and knee flexion (93.70 ± 7.47° vs. 82.92 ± 11.14°, p = 0.004) between groups. These findings suggest that subtalar pronation, assessed by NDT, and calcaneal eversion could be indicators of higher susceptibility to ACL injuries in female basketball players.
Myocardial Work Assessment in Patients after Coronary Artery Bypass Grafting during Cardiac Rehabilitation
Authors: Perone Francesco , Ancona Roberta , di Stasio Fausto , La Gambina Vito , Comenale Pinto Salvatore
Published: December 2023

Background: Myocardial work is an innovative echocardiographic tool to assess left ventricular performance. Emerging data have shown the added value of this method for evaluating cardiac function compared to traditional echocardiographic parameters and global longitudinal strain. However, few studies are present in the literature about the role of myocardial work during cardiac rehabilitation. Our aim was to assess the impact of a rehabilitation program on myocardial work indices in patients with preserved left ventricular ejection fraction and after coronary artery bypass grafting. In addition, we assessed the correlation between baseline myocardial work indices and their change after cardiac rehabilitation, establishing an optimal cut-off value to predict the improvement. Methods: An observational, single-center, and prospective study was conducted. We enrolled patients referred to cardiac rehabilitation after coronary artery bypass grafting and with preserved left ventricular ejection fraction. Before and after the cardiac rehabilitation program, a comprehensive patient assessment was performed, including traditional transthoracic echocardiography, myocardial work analysis, and a six-minute walk test. Results: Eighty-four patients were enrolled; the mean age was 67.96 (±7.42) years and 78.6% were male. The left ventricular ejection fraction was preserved in all patients, and the global longitudinal strain was −16.18 ± 2.55%, the global work index was 1588.56 ± 345 mmHg%, the global constructive work was 1771.27 ± 366.36 mmHg%, the global wasted work was 105.8 ± 72.02 mmHg%, and the global work efficiency was 92.63 ± 3.9% at baseline. After the cardiac rehabilitation program, the global work index, the global constructive work, and the six-minute walk test improved significantly (1588.56 ± 345 vs. 1960.2 ± 377.03 mmHg%, p-value < 0.001; 1771.27 ± 366.36 vs. 2172.01 ± 418.73 mmHg%, p-value < 0.001; 70.71 ± 40.2 vs. 437.5 ± 108.70 m, p-value < 0.001, respectively). Conclusions: Myocardial work indices, specifically global work index and global constructive work, improve after cardiac rehabilitation program in patients undergoing coronary artery bypass grafting with preserved left ventricular ejection fraction.
Telerehabilitation for Stroke: A Personalized Multi-Domain Approach in a Pilot Study
Authors: Federico Sara , Cacciante Luisa , De Icco Roberto , Gatti Roberto , Jonsdottir Johanna , Pagliari Chiara , Franceschini Marco , Goffredo Michela , Cioeta Matteo , Calabrò Rocco Salvatore , Maistrello Lorenza , Turolla Andrea , Kiper Pawel ,   on behalf of RIN_TR_Group
Published: December 2023

Stroke, a leading cause of long-term disability worldwide, manifests as motor, speech language, and cognitive impairments, necessitating customized rehabilitation strategies. In this context, telerehabilitation (TR) strategies have emerged as promising solutions. In a multi-center longitudinal pilot study, we explored the effects of a multi-domain TR program, comprising physiotherapy, speech therapy, and neuropsychological treatments. In total, 84 stroke survivors (74 analyzed) received 20 tailored sessions per domain, addressing individual impairments and customized to their specific needs. Positive correlations were found between initial motor function, cognitive status, independence in activities of daily living (ADLs), and motor function improvement after TR. A lower initial health-related quality of life (HRQoL) perception hindered progress, but improved ADL independence and overall health status, and reduced depression correlated with a better QoL. Furthermore, post-treatment improvements were observed in the entire sample in terms of fine motor skills, upper-limb functionality, balance, independence, and cognitive impairment. This multi-modal approach shows promise in enhancing stroke rehabilitation and highlights the potential of TR in addressing the complex needs of stroke survivors through a comprehensive support and interdisciplinary collaboration, personalized for each individual’s needs.
Spark Plasma Sintering of Fine-Grained WC-Co Composites
Authors: Wachowicz Joanna , Dembiczak Tomasz , Fik Joanna , Bałaga Zbigniew , Kruzel Robert , Náprstková Nataša , Kuśmierczak Sylvia
Published: December 2023

Cemented carbides WC-Co are one of the basic tool materials. They constitute over half of the currently used tools intended for machining. The main advantages of WC-Co cemented carbides are high hardness and abrasion resistance. The properties of WC-Co sinters depend mainly on the content of the binding phase, the sintering method and the grain size of the powder from which the sinters were made. The aim of this study was to produce fine-grained WC-Co composites using SPS (spark plasma sintering) technology, as well as examine the effect of the applied technology on the basic properties of WC-Co sinters: microstructure, hardness, phase composition, compaction degree and tribological properties. In the processes carried out, no additives affecting the reduction in grain growth were used. Sintering was conducted at a temperature of 1200 °C with a holding time of 10 min. The process occurred under a load of 100 MPa. Finally, the samples were cooled in a vacuum of 10−6 mbar. We measured the hardness using a Vickers hardness tester. We took hardness measurements along the diameter of the sintered samples. In order to ascertain the fracture toughness (KIC), we measured the radial crack length around the Vickers indentation and applied Shetty’s formula. The tribological tests were carried out with a tribotester using the T-01 ball-on-disc method. The obtained data enabled the characterization of the wear process of the tested materials.
Eigenvibrations of Kirchhoff Rectangular Random Plates on Time-Fractional Viscoelastic Supports via the Stochastic Finite Element Method
Authors: Kamiński Marcin , Guminiak Michał , Lenartowicz Agnieszka , Łasecka-Plura Magdalena , Przychodzki Maciej , Sumelka Wojciech
Published: December 2023

The present work’s main objective is to investigate the natural vibrations of the thin (Kirchhoff–Love) plate resting on time-fractional viscoelastic supports in terms of the Stochastic Finite Element Method (SFEM). The behavior of the supports is described by the fractional order derivatives of the Riemann–Liouville type. The subspace iteration method, in conjunction with the continuation method, is used as a tool to solve the non-linear eigenproblem. A deterministic core for solving structural eigenvibrations is the Finite Element Method. The probabilistic analysis includes the Monte-Carlo simulation and the semi-analytical approach, as well as the iterative generalized stochastic perturbation method. Probabilistic structural response in the form of up to the second-order characteristics is investigated numerically in addition to the input uncertainty level. Finally, the probabilistic relative entropy and the safety measure are estimated. The presented investigations can be applied to the dynamics of foundation plates resting on viscoelastic soil.