bluuebunny/crossref_metadata_2025 huggingface dataset:
КОРИСТУВАЦЬКИЙ КОНТЕНТ. СТРУКТУРНА МОДЕЛЬ ФОРМУВАННЯ ДОВІРИ
Authors: Лебеденко С. О. , Гнітецький Є. В.
Published: October 2023

За останні кілька років маркетинг у соціальних мережах став основним маркетинговим інструментом для компаній. Останні дані показують, що 90% компаній зазвичай використовують соціальні мережі для просування свого бізнесу. Компанії використовують соціальні медіа не лише для цілей цифрової реклами, але й для того, щоб керувати своїми стосунками з клієнтами, залучати їх і використовувати їхні ідеї та інформацію для інноваційної продукції та управління брендом. Надзвичайна популярність соціальних медіа, таких як Facebook, Twitter, YouTube, Instagram, надало компаніям великий стимул використовувати соціальні медіа для створення цінності. Контент, створений користувачами (user-generated content UGC) – це опублікований в Інтернеті контент, який «створено поза професійними процедурами та практиками». UGC, пов’язаний з брендом, відіграє центральну роль у розвитку соціальних взаємовідносин з брендом. Дане дослідження об’єднало кілька теорій, щоб запропонувати інтегровану структурну S–O–R моделі з урахуванням когнітивно-афективно-конативних аспектів. Розроблена модель додає новий теоретичний напрям дослідженням довіри до UGC. Проведене дослідження допоможе дослідникам у вирішенні проблеми зміщення оцінок структурних змінних через виключення одного або кількох релевантних факторів з моделі, що може призвести до непослідовних і упереджених результатів. Таким чином, це дослідження робить внесок у існуючі дослідження довіри до UGC. Водночас наведене дослідження вивчає задоволеність життям і задоволеність Інтернетом як два основні механізми обміну онлайн-контентом. Задоволеність користувачів (тобто задоволеність Інтернетом) розглядалася як важлива змінна, що впливає на поведінку користувачів з точки зору успіху інформаційної системи і теорії очікування-непідтвердження. Наведене дослідження розглядало Інтернет як загальний інструмент для обміну онлайн-контентом. Не було обмежень типу онлайн-платформ. (віртуальні спільноти, сайти соціальних мереж). Дослідження було зосереджено на контенті, який користувачі створюють самостійно і яким діляться самостійно в Інтернеті. Незважаючи на це, користувачі можуть репостити або ділитися вмістом, який вони знайшли в інших місцях в Інтернеті.
ДОСЛІДЖЕННЯ СПРИЙНЯТТЯ СТУДЕНТАМИ СПРЯМОВАНОСТІ СТАНДАРТІВ ОСВІТИ З МЕТОЮ ПОБУДОВИ СИСТЕМИ ФОРМУВАННЯ ЦІННІСНИХ ОРІЄНТИРІВ ОСОБИСТОСТІ
Authors: Шульгіна Л. М. , Гнітецький Є. В. , Бажеріна К. В.
Published: October 2023

Метою даної статті є дослідження сприйняття студентами ступеня спрямованості існуючих освітніх стандартів на формування та розвиток цінностей особистості, а також формулювання пропозицій щодо впровадження системного підходу до розробки освітніх стандартів як фактору удосконалення існуючого комплексу цінностей особистості. Для досягнення цієї мети була використана методологія, що включала комплекс методів, а саме: кабінетний аналіз, порівняння, дедукція та індукція; анкетування; дисперсійний, дискримінантний, факторний і морфологічний аналізи. Респондентів сегментували за критеріями «наявність груп значень» – «спряженість груп значень зі змінними». Визначено низький рівень морально-етичних цінностей у студентів, тобто тієї частини суспільства, яка вважається найбільш активною та прогресивною. Обґрунтовано місце освіти в макросередовищі країни. Розроблено ряд заходів щодо вирішення проблеми підвищення ціннісного рівня в усіх учасників навчально-виховного процесу з метою зменшення розриву між темпами технічного та гуманітарного розвитку суспільства.
РОЗРОБЛЕННЯ МОДЕЛІ КОМЕРЦІАЛІЗАЦІЇ ІННОВАЦІЙ НА ОСНОВІ ВРАХУВАННЯ «ВУЗЬКИХ» МІСЦЬ
Authors: Сагер Л. Ю. , Сигида Л. О. , Бондаренко А. Ф.
Published: October 2023

Проведені дослідження спрямовані на формування авторської моделі комерціалізації інновацій, що ґрунтується на перевагах і «вузьких» місцях існуючих моделей. Розроблена модель передбачає проходження чотирьох стадій – підготовчу стадію, бізнес-стадію, стадію розроблення та стадію впровадження. Визначено, що підготовча стадія спрямована на формування концепції інновації. Бізнес-стадія є стадією презентації бізнес-пропозиції та досягнення домовленостей між всіма учасниками моделі, що засвідчується укладанням контрактів. Стадія розроблення передбачає представлення перевіреного прототипу інновації, а стадія впровадження – початок її масового виробництва. Крім того, у моделі показано напрямки подолання «вузьких» місць, пов’язаних із відособленістю процесу комерціалізації від учасників; із недостатньою увагою до думок і бажань споживачів; із захистом прав інтелектуальної власності на інноваційну продукцію.
ОПТИМАЛЬНЕ КЕРУВАННЯ ВЕЛИЧИНОЮ ЗНИЖКИ НА БРЕНД ФІРМОЮ РОЗДРІБНОЇ ТОРГІВЛІ
Authors: Мазуренко М. C. , Фартушний І. Д.
Published: October 2023

Особливу увагу у цій статті присвячено знижці, як ціновому специфічному фактору впливу на попит підприємства. Метою даної роботи стає формалізація та побудова специфічного апарату по встановленню знижки на продукцію, що представляє собою один із основних факторів керування попитом підприємства. Відповідно до поставленої мети створюється відповідна структуризація та формалізація необхідного матеріалу, представлення взаємовідношень та гіпотез у вигляді моделі та створення прикладного апарату для вирішення її. Досліджується сукупність економічних взаємовідносин, що виникають між продавцями та покупцями на ринку в роздрібній торгівлі, якому найбільш властиве використання фактору знижки, предметом дослідження є регулювання ціною, шляхом надання знижки, фірмою та її взаємозв’язок із реакцією споживачів в умовах невизначеності.
Conservation of the Nara Palace Site – World Cultural Heritage in Japan and Discussion on the so-called “Architectural Heritage DNA”
Authors: LE Vinh An , NGUYEN Thi Kim Nhung
Published: October 2023

This paper introduces in general the conservation work of the Nara Palace Site in Japan carried out by Japanese conservation experts. Concerning conservation theory, this paper focused on the international conventions in which the 1994 Nara Document on Authenticity plays an important role in contributing to Japanese cultural heritage conservation achievements with the expansion of Cultural diversity and Heritage diversity, the Values and Authenticity conceptions which proposed a new orientation in terms of awareness and openness broader vision in identifying architectural heritage values. Regarding, based on the 1994 Nara Document on Authenticity, the 1964 Venice Charter, and the 1999 Bura Charter, we try to propose a new concept of the so-called “Architectural Heritage DNA” and its potential structure, providing a simple formula for the authentic values appraisal of the heritage conservation works. In addition, we also try to supplement the concept of Inheritance between Conservation and Development which is the lack of international conventions of cultural heritage conservation. The viewpoints expressed in this paper are based on the most common concepts of international conventions but interpreted in a new-understandable, practical, and highly applicable way possible to the current socio-political situation and in the future, as well.
Activation of the Immune System against the Background of Its Imbalance Caused by Agents of Radiation Nature
Authors: Vagin Konstantin , Gaynutdinov Timur , Rakhmatullina Gulnaz , Nizamov Rustam , Gallyamova Marina
Published: October 2023

By conjugating a radioantigen (o-quinone) with a protein - Esherichia coli endotoxin, a stable polyantigen complex-protein-quinoid radioantigen (BChA) - o-quinone imine (C6H10O-NH - protein) was obtained, including haptenic (o-quinone) and protein part of the antigenic complex (Esherichia coli endotoxin).

Using the above components (hyperimmune antiradiotoxic serum, ethanol extract of the Vita-Force apiphitopreparation, metabolic products of Bifidobacterium bifidum, Bacillus subtilis and a highly dispersed fraction of bentonite), a polyfunctional composition «Polyapisogen» was compiled, including polyglobulins (500 cm3 per 1 L),500 cm3 of 4% ethanol extract of the Vita-Force apiphitopreparation, in which a highly dispersed fraction of bentonite (20 g), metabolism products (2 g powder) and Bacillus subtilis metabolism products (4 g) are dissolved.

The polyfunctional preparation obtained by the above-described technology is used as an activator of the immune system against the background of its imbalance induced by agents of a radiogenic nature.

It was found that a single subcutaneous injection of the drug at a dose of 25 mg / kg of live weight (dry matter basis) had a radioprotective effect increasing the survival rate of affected animals upto 75%.

The studies carried out can serve as a basis for the creation of polyfunctional radioprotective, immunoprotective and bioprotective agents not only for isolated, but also for combined lesions of the body by agents of radiogenic and non-radiogenic nature using polyglobulins, convalescent serums, bee products, metabolic products of probiotic microorganisms and mineral sorbents.
Comprehensive Study of Fuel Cell Hybrid Electric Vehicles: Classification, Topologies, and Control System Comparisons
Authors: Ragab Ahmed , Marei Mostafa I. , Mokhtar Mohamed
Published: October 2023

The utilization of fuel cells (FC) in automotive technology has experienced significant growth in recent years. Fuel cell hybrid electric vehicles (FCHEVs) are powered by a combination of fuel cells, batteries, or/and ultracapacitors (UCs). By integrating power converters with these power sources, the FCHEV system can overcome the limitations of using them separately. The performance of an FCHEV is influenced by the efficiency of the power electronics converter controller, as well as the technical efficiency of the power sources. FCHEVs need intricate energy management systems (EMSs) to function effectively. Poor EMS can lead to low efficiency and accelerated fuel cell and battery degradation. The literature discussed various types of EMSs, such as equivalent consumption minimization strategy, classical PI controller, fuzzy logic controller, and Mutative Fuzzy Logic Controller (MFLC). However, comparing different EMSs can be challenging due to the varying vehicle and system parameters, which might lead to false claims being made regarding system performance. This research aims to categorize and discuss the various topologies of FCHEVs, highlighting their pros and cons, and comparing several EMSs based on performance metrics such as State of Charge (SOC) and FC deterioration.
O impacto da mortalidade por causas externas na esperança de vida nos municípios produtores de petróleo da Bacia de Campos/RJ
Authors: Xavier Moraes Mayara , De Souza Joseane , Passarelli-Araujo Hisrael
Published: October 2023

O crescimento da mortalidade por causas externas no Brasil tem se tornado objeto de preocupação entre pesquisadores e formuladores de políticas públicas nos últimos anos. No âmbito dos municípios produtores de petróleo da Bacia de Campos/RJ, embora muito se conheça sobre os padrões migratórios nesta região que é considerada um aspecto relevante da urbanização brasileira, as informações sobre os demais componentes da dinâmica demográfica ainda são escassas. Este estudo amplia a discussão sobre as disparidades de saúde na região, investigando o impacto da mortalidade por causas externas na expectativa de vida da população entre os períodos 2010-2014 e 2015-2019. Utilizando dados de óbitos e estimativas populacionais, foram construídas tabelas de múltiplo decremento para avaliar o ganho na expectativa de vida se a mortalidade por causas externas fosse excluída do risco de óbito e como esses ganhos diferem entre homens e mulheres. Também avaliaram-se cenários distintos de redução da mortalidade por homicídios e acidentes de trânsito, as duas principais causas de morte do grupo de causas externas. Os resultados revelam ganhos médios substanciais de expectativa de vida para ambos os sexos, especialmente para os homens. As simulações realizadas mostraram que a redução da mortalidade por acidentes de trânsito e homicídios pode trazer ganhos importantes para a população, destacando a relevância de investir em políticas públicas que combatam as causas externas de mortalidade em uma região marcada por desigualdades sociais e econômicas.
Hubungan Tingkat Depresi dengan Indeks Massa Tubuh (IMT) pada Remaja Sekolah Menengah Pertama (SMP) Selama Masa Pandemi COVID-19 di Surakarta
Authors: Indi Aisyah Jauza Adiba , Andhika Trisna Putra  , Septin Widiretnani
Published: October 2023

Pendahuluan: Penyebaran COVID-19 di Indonesia telah berlangsung sejak bulan Maret 2020. Di Indonesia, gangguan mental emosional pada anak usia lebih dari 15 tahun memiliki prevalensi yang meningkat dari 6% di tahun 2013 menjadi 9,8% di tahun 2018. Sedangkan anak usia 15 tahun ke atas dengan kejadian depresi memiliki prevalensi sebesar 6,1%. Pada penelitian sebelumnya, menunjukan bahwa masalah psikologis yang paling banyak dialami oleh siswa selama masa pandemi COVID-19 ini salah satunya adalah depresi. Penelitian ini bertujuan untuk mengetahui hubungan antara tingkat depresi dengan Indeks Massa Tubuh (IMT) pada remaja Sekolah Menengah Pertama (SMP) di Surakarta selama masa pandemi COVID-19.
Metode: Penelitian ini merupakan penelitian observasional analitik dengan pendekatan cross sectional yang dilakukan pada Bulan Mei-Juni 2022 di SMP Muhammadiyah 5 Surakarta, SMPN 4 Surakarta, dan SMP Batik Program Khusus Surakarta. Metode pengambilan data menggunakan purposive sampling dengan kuesioner BDI II dan didapatkan 103 sampel siswa kelas VII dan VIII. Analisis data dilakukan dengan uji normalitas Kolmogorov-Smirnov dan uji hipotesis menggunakan uji korelasi Spearman atau uji Mann-Whitney menggunakan SPSS.
Hasil: Data hasil penelitian tingkat depresi dengan indeks massa tubuh (IMT) diperoleh nilai p = 0.045 (p < 0.05) dan nilai Correlation Coefficient (r) didapatkan hasil -0.198 artinya tedapat hubungan antara tingkat depresi dengan indeks massa tubuh (IMT) dengan korelasi antar variabel sangat lemah dan hubungan tidak searah karena nilai r negatif.
Kesimpulan: Terdapat hubungan antara tingkat depresi dengan Indeks Massa Tubuh  (IMT) pada remaja Sekolah Menengah Pertama (SMP) selama masa pandemi COVID-19 di Surakarta.
Türkiye’nin 100. Yılında Sosyal Sermaye ve İnovasyonun Bölgesel Açıdan Durumu Üzerine Mekânsal Ekonometrik Bir Analiz
Authors: OGUZTURK Bekir Sami , KOÇ Recep
Published: October 2023

Çalışmanın amacı, sosyal sermaye ve inovasyonun bölgesel kalkınmada nasıl bir etkisinin olduğunu araştırmaktır. Bu doğrultuda mekânsal bir model geliştirilmiştir. Bu model hem yatay kesit hem de zaman serisi içerdiğinden dolayı: sosyal sermaye ve inovasyonun bölgesel kalkınma göstergesi olarak kişi başı gayri safi milli hasılayı mekânsal olarak etkilediği varsayılmış ve bu doğrultuda mekânsal panel veri analizi yapılmıştır. Çalışmanın verileri 2007-2021 yılları arasında TR42 Doğu Marmara Kalkınma Bölgesine (MARKA) bağlı illerin sosyal sermaye endeksi, inovasyon endeksi ve kişi başı gayri safi milli hâsıla verilerinden elde edilmiştir. Sosyal sermaye ve inovasyon kavramlarının nicel özellik kazanması için öncelikle bunların alt belirleyicileri tespit edilmiştir. Bunların, çalışılan bölge için ulaşılabilen belirleyicileri ile sosyal sermaye ve inovasyon endeksleri ilgili yıllar içerisinde elde edilmiştir. Çalışma sonucunda hem bölge genelinde hem de iller arasında mekânsal bağımlık tespit edilmiştir. Ayrıca mekânsal etki modele eklenince modelin Sabit Etkili Mekânsal Hata Panel Veri Analizi (SEM) ile analizin yapılması kabul edilmiştir. Sonuç olarak inovasyonun kişi başı geliri etkilemesi anlamlı bulunmazken sosyal sermayenin kişi başı geliri etkilemesi anlamlı bulunmuştur. Sosyal sermayedeki bir birimlik artışın, kişi başı gayri safi milli hasılayı 2.530 dolar olarak azalttığı tespit edilmiştir.
Accelerating multielectron reduction at CuxO nanograins interfaces with controlled local electric field
Authors: Ye Ruquan , Guo Weihua , Zhang Siwei , Zhang Junjie , Wu Haoran , Ma Yangbo , Song Yun , Cheng Le , Chang Liang , Li Geng , Liu Yong , Wei Guodan , Gan Lin , Zhu Minghui , Xi Shibo , Wang Xue , Yakobson Boris , Tang Ben Zhong
Published: October 2023

Regulating electron transport rate and ion concentrations in the local microenvironment of active site can overcome the slow kinetics and unfavorable thermodynamics of CO2 electroreduction. However, simultaneous optimization of both kinetics and thermodynamics is hindered by synthetic constraints and poor mechanistic understanding. Here we leverage laser-assisted manufacturing for synthesizing CuxO bipyramids with controlled tip angles and abundant nanograins, and elucidate the mechanism of the relationship between electron transport/ion concentrations and electrocatalytic performance. Potassium/OH− adsorption tests and finite element simulations corroborate the contributions from strong electric field at the sharp tip. In situ Fourier transform infrared spectrometry and differential electrochemical mass spectrometry unveil the dynamic evolution of critical *CO/*OCCOH intermediates and product profiles, complemented with theoretical calculations that elucidate the thermodynamic contributions from improved coupling at the Cu+/Cu2+ interfaces. Through modulating the electron transport and ion concentrations, we achieve high Faradaic efficiency of 81% at ~900 mA cm−2 for C2+ products via CO2RR. Similar enhancement is also observed for nitrate reduction reaction (NITRR), achieving 81.83 mg h−1 ammonia yield rate. Coupling the CO2RR and NITRR systems demonstrates the potential for valorizing flue gases and nitrate wastes, which suggests a practical approach for carbon-nitrogen cycling.
The Innovation Effect of Green Finance Reform-Based on the green innovation vitality perspective
Authors: Mao Jinhuang , Chen Jin
Published: October 2023

In order to develop green finance and realize the coordinated development of the environment and the economy, China set up the Green Finance Reform and Innovation Pilot Zone in 2017. This paper takes the green financial reform and innovation pilot zones set up in China as an exogenous shock, takes the five provinces that set up green financial reform and innovation pilot zones in 2017 as the experimental group, and takes the other 25 provinces that did not set up or set up green financial reform and innovation pilot zones later as the control group, and constructs panel data for the period of 2012-2021 for the 30 provinces in China (excluding Hong Kong, Macao , Taiwan, and Tibet) panel data, using synthetic control method, double difference model and mediation effect model to analyze the impact and mechanism of green financial policies on China's green innovation vitality. It is found that the implementation of green financial policies can significantly enhance the level of green innovation vitality in China. The results of the heterogeneity study show that the positive effect of green financial policies is greatest in the east, followed by the west, while the positive promotion effect is not obvious in the central region. The results of the mechanism study indicate that green financial policies promote the level of green innovation vitality through government incentive behavior. The findings of the study can provide effective support for the further promotion of the pilot policy and provide important insights for green development.
Free-ranging dog’s effects on the activity pattern of prey species in areas from Cerrado and Atlantic Forest, Brazil
Authors: Moretto Letícia Rodrigues , Fujioka Bárbara Mitsuko Zukeram , Silva Letícia Vitória da , Olifiers Natalie , Riski Letícia Lütke , D'Andrea Paulo Sérgio , Panosso Alan Rodrigo , Kays Roland , Dias Viviane Brito , Bianchi Rita de Cassia
Published: October 2023

One of the significant challenges in ecology today is comprehending the more intricate networks of interactions, as seen in interpreting relationships between predators and prey. In the case of exotic predators, their prey species might not associate such animals with the risk of predation, leading to a lack of development of anti-predation strategies against them. Thus, the objective of this study is to understand the effects that domestic dogs (Canis familiaris) can have on prey such as agoutis (Dasyprocta), armadillos (Cabassous spp., Dasypus novemcinctus, Euphractus sexcinctus), giant-anteaters (Myrmecophaga tridactyla) and pacas (Cuniculus paca). For this, we will: 1) compare the activity patterns of the species, 2) compare the activity pattern of the prey species in places with high and low abundance of dogs and 3) analyze whether there is temporal avoidance of dogs by prey, comparing with other natural predators (Chrysocyon brachyurus, Leopardus pardalis, and Puma concolor), as well as non-predator species that will serve as a control. Our results demonstrated that the prey species are not altering their activity patterns, nor do they exhibit temporal avoidance of dogs compared to the other species in the study. This may be occurring due to 1) high energy costs associated with changing the activity pattern; 2) the anti-predation strategies used for other species can already be efficient against domestic dogs, and 3) the avoidance can be spatial. Furthermore, novel methods of data analysis are required to gain a better understanding of these effects.
The effect of CMTM6 on the anti-HCC efficacy of iNKT cells via stabilizing PD-L1 in an experimental investigation
Authors: Teng Jingfang , Wang Yaqi , Zhang Min , Li Zheng , Liu Qiyao , Lv Wenping , Meng Ming
Published: October 2023

Background CMTM6 is a key regulator of PD-L1. The purpose of this study is to see whether CMTM6 impacts iNKT cells infiltration into hepatocellular carcinoma (HCC) and whether CMTM6 knockout promotes iNKT cells activation via the PD-1/PD-L1 signaling axis, hence impacting anti-HCC efficacy.
Methods Immunohistochemistry and multiplex fluorescence immunohistochemistry were conducted to evaluate the expression of CMTM6, PD-1, PD-L1, and iNKT (CD3+CD56+) cells. Flow cytometry (FCM), enzyme-linked immunosorbent assay (ELISA), cell proliferation, killing, colony formation assay, and wound healing assay were applied to investigate the effect of CMTM6 knockout on the anti-HCC efficacy of iNKT cells.
Results In HCC tumor tissues, there's far more CMTM6, PD-L1, and PD-1 expression. PD-L1 and PD-1 were both positively associated with CMTM6. iNKT cells infiltration was reduced, whereas PD-1 expression was increased. Infiltration of iNKT cells was reduced by increased CMTM6 expression. CMTM6 knockout inhibited the proliferation, invasion, and migration of HepG2 cells by downregulating the PD-1/PD-L1 signaling axis and promoting the secretion of IFN-γ, Perforin, and Granzyme B of iNKT cells, boosting their killing effect, and inhibiting the proliferation, invasion, and migration of HepG2 cells.
Conclusions Our findings showed that CMTM6 was more abundant in HCC tumor tissues than adjacent tissues. Knocking out CMTM6 could boost iNKT cells activation and improve anti-HCC efficacy by blocking the PD-1/PD-L1 signaling axis, which may serve as a potential therapeutic target for HCC
Impact of Affordable Care Act provisions on the racial makeup of patients enrolled at a Deep South, high-risk breast cancer clinic
Authors: Tinglin Jillian , McLeod M. Chandler , Williams Courtney P. , Tipre Meghan , Rocque Gabrielle , Crouse Andrew B. , Krontiras Helen , Gutnik Lily
Published: October 2023

Purpose
Black women are less likely to receive screening mammograms and are at a higher lifetime risk for developing breast cancer compared to their White counterparts. Affordable Care Act (ACA) provisions decreased cost sharing for women’s preventive screening, potentially mitigating screening disparities. We examined enrollment of a high-risk screening program before and after ACA implementation stratified by race.
Methods
This retrospective, quasi-experimental study examined the ACA’s impact on patient demographics at a high-risk breast cancer screening clinic from 02/28/2003–02/28/2019. Patient demographic data were abstracted from electronic medical records and descriptively compared in the pre- and post-ACA time periods. Interrupted time series (ITS) analysis using Poisson regression assessed yearly clinic enrollment rates by race using incidence rate ratios (IRR) and 95% confidence intervals (CI).
Results
2,767 patients enrolled in the clinic. On average, patients were 46 years old (SD, ± 12), 82% were commercially insured, and 8% lived in a highly disadvantaged neighborhood. In ITS models accounting for trends over time, Prior to ACA implementation, White patient enrollment was stable (IRR 1.01, 95% CI 1.00-1.02) while Black patient enrollment increased at 13% per year (IRR 1.13, 95% CI 1.05–1.22). Compared to the pre-ACA enrollment period, the post-ACA enrollment rate remained unchanged for White patients (IRR 0.99, 95% CI 0.97–1.01) but decreased by 17% for Black patients (IRR 0.83, 95% CI 0.74–0.92).
Conclusion
Black patient enrollment decreased at a high-risk breast cancer screening clinic post-ACA compared to the pre-ACA period, indicating a need to identify factors contributing to racial disparities in clinic enrollment.
Correlation-based network analysis combined with machine learning techniques highlights rosmarinic acid biosynthesis activation in tuber of potato plants corresponding to water recovery treatment
Authors: Maruenda Helena , Toubiana David
Published: October 2023

Water-deficit, as a consequence of elongated drought periods due to current climate trends, is one of the most imminent abiotic stresses limiting crop productivity worldwide. Also, the production of potato – the world’s fourth most important crop – is challenged by the effects of water-deficit. To investigate metabolic pathway activity in the potato tuber subjected to water-deficit, a correlation-based network analysis combined with machine-learning techniques approach was conducted on a subset of samples of a potato association panel, grown in an augmented block design under normal irrigation and water-deficit conditions in Ica, Peru. Metabolic profiles for all samples of the subset were generated using a cold injection GC-TOF-MS platform identifying unequivocally 685 metabolites. Samples were separated into positive spectrum (PS) and negative spectrum (NS) groups based on their projection on principal component 2 (PC2) using principal component analysis. Correlation networks showed a strong discrepancy of edge number between the PS and NS correlation network (143,478 vs. 185,565 edges). Correlation-based network analysis combined with machine learning techniques highlighted the activity of metabolic pathways centered around tyrosine and α-ketoglutarate suggestive for an increased activity of the rosmarinic acid biosynthesis I and II pathways in the potato tuber. Our study suggests that the potato tuber copes with drought stress by rewiring its metabolic network and boosting rosmarinic acid biosynthesis to counteract ROS activity and by that ensuring the onset of the next life cycle.
Robotic Assisted Orbital Surgery for resection of advanced periocular tumours– a case series report on the feasibility, safety and outcome
Authors: Malik Mohsan , Daniel Claire , FAULKNER JACK , Uddin Jimmy , Arora Asit , Jeannon Jean Pierre
Published: October 2023

Purpose Orbital surgery benefits from well-designed instrumentation that offers gentle tissue manipulation, high manoeuvrability and control. Nevertheless, in confined spaces, tissue manipulation must be accomplished with exceptionally high accuracy and precision. This is where robotic surgery offers an advantage. We aimed to evaluate a robotic-assisted surgical system's feasibility, safety and outcome in assisting tumour clearance.Patients and methods A case series of patients with advanced periocular tumours undergoing robotic-assisted globe-sparing resection was performed using the DaVinci XI system (intuitive surgical, inc). Institutional ethics and multidisciplinary approval were sought in all cases.Results Four patients with advanced periocular tumours underwent robotic-assisted orbital surgery at a mean age of 63 years (range 42–86). Two patients were diagnosed with squamous cell carcinoma, and two had basal cell carcinoma. One patient was found to have positive lymph nodes at the time of surgery and underwent simultaneous parotidectomy and lymph node clearance. Clear resection of the primary tumour was achieved in all patients; three patients underwent further resection due to narrow margins prior to reconstruction. Patients were follow-up for at least one year, and three remained disease-free. One patient with pre-existing extra-orbital disease developed metastatic disease four months post-op. All patients preserved vision peri-operatively, with no complaints of diplopia. Moderate ocular surface disease was noted in two patients.Conclusion Our series highlights the potential advantage of three-dimensional optics, multi-directional instrumentation and motion scaling technology to achieve globe-sparing tumour resection in advanced periocular tumours. However, further robotic instrumentation development is required for orbital surgery.
Association Between Physical Activity in Its Different Domains and Eating Habits in Female Breast Cancer Survivors
Authors: Caldeira Daniela T. , Vanderlei Luiz Carlos M. , Palma Mariana R. , Ribeiro Fernanda E. , Tebar William R. , Mota Jorge , Ferrari Gerson , Tosello Giuliano T. , Christofaro Diego G. D.
Published: October 2023

Healthy eating patterns associated with physical activity (PA) are important factors for the survival of breast cancer survivors. However, little is known about whether the different domains of PA (occupational, leisure/sport and commuting) can interfere with healthy eating. For this, 128 breast cancer survivors were evaluated. The socioeconomic class was analyzed using the questionnaire of the Brazilian Association of Research Companies (ABEP). The eating habits and the frequency of weekly food intake were assessed through the Block questionnaire, while the level of PA through the Baecke questionnaire and the time spent on sedentary behavior through the participants' reports. It was found that breast cancer survivors with higher levels of leisure / sport PA presented higher consumption of fruits (β = 0.32; CI = 0.02; 0.62), cereals (β = 0.79; CI = 0.47; 1.10) and white meat (β = 0.31; CI = 0.05; 0.58), similar results were observed with displacement PA. Higher levels of PA were related to higher consumption of vegetables (β = 0.28; CI = 0.05; 0.50) and lower consumption of candies (β = -0.33; CI = -0.59; -0.08). It is concluded that breast cancer survivors who practice more PA in their different domains had a higher consumption of healthy foods.
Chemical composition and biological activities Assessment (antioxidant, antimicrobial, anti-inflammatory and healing wounds activities) of crude extract of roots of Paeonia mascula subsp. atlantica (Coss.) Greuter & Burdet = (Paeonia algeriensis Chabert)
Authors: wafa Nouioua , sofiane Gaamoune , ouarda Djaout , nouioua wafa
Published: October 2023

Background:Paeonia algeriensisChabert is an Algerian endemic species habitually used by local people in traditional medicine. This study tried to express phytochemicals composition and biological activities of the species. CG/Ms was used to determine molecular composition, antioxidant activity was assessed by three tests (DPPH, reducing power and lipid peroxidation), antimicrobial activity was tested with four bacterial strains and one yeast, the anti-inflammatory activity was carried out by the human red blood cell (HRBC) membrane stabilization method and theIn-vivohealing wounds activity by excision model.Results:The result show a perfect antioxidant activity, a powerful antibacterial effect an important anti-inflammatory power at a low concentration and total healing wounds effect in just 13 days of experiment.Conclusions:The above results indicate that the roots ofPaeonia masculahave great potential in the development of new drugs.
Correlation between 8-OHdG and disease-free survival in patients with esophageal squamous cell carcinoma following neoadjuvant chemotherapy
Authors: Kunimitsu Atsushi , Fujishima Fumiyoshi , Sasano Hironobu , Mozumi Takeru , Higashimoto Iku , Akaishi Ryujiro , Koseki¹ Ken , Ishida Hitoraka , Ozawa¹ Yohei , Sato Chiaki , Okamoto¹ Hiroshi , Taniyama¹ Yusuke , Kamei¹ Takashi , Suzuki² Takashi
Published: October 2023

Background: Esophageal squamous cell carcinoma (ESCC) is an aggressive malignancy. Neoadjuvant chemotherapy (NAC) followed by surgery is the standard treatment strategy for the patients with stage II or III ESCC, but many develop resistance to treatment. Recent studies have suggested a correlation between therapeutic resistance and enhancement of the nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway in cancer cells but its details, including the antioxidant pathways which regulate the oxidative stress response after NAC, are unknown. Therefore, in this study, we investigated the status of heme oxygenase-1 (HO-1), a target of the Nrf2 pathway, and 8-hydroxy-2-deoxyguanosine (8-OHdG), an oxidative stress marker, in patients with ESCC who underwent NAC, and explored treatment strategies with relation to the findings and clinical outcomes and its correlation to clinical or histologic responsiveness.
Methods: HO-1 and 8-OHdG were immunolocalized in 99 patients with ESCC who received NAC with cisplatin and 5-fluorouracil (CF) prior to esophagectomy. Among them, 53 had lymph node metastasis at the time of surgery.
Results: Kaplan­­–Meier curves revealed that overall survival (OS) and disease-free survival (DFS) were both significantly prolonged in patients with ESCC and low HO-1 primary tumor H-score compared to those with high scores. DFS was significantly prolonged in the group with low 8-OHdG levels compared to that with high scores. Multivariate analysis demonstrated that high 8-OHdG levels adversely affected DFS in an independent fashion. In addition, Univariate analysis showed significantly prolonged OS and DFS in ESCC patients with higher histological effects of NAC, but multivariate analysis revealed that only regional lymph node metastasis (pN) was significantly correlated with OS and DFS.
Conclusions: HO-1 and 8-OHdG levels in the primary tumor were significantly correlated with NAC response in patients with ESCC. In addition, a relatively high 8-OHdG level in tumors after NAC could predict poor DFS.
Trial registration: Accession No. 2022-1-779. The trial was retrospectively registered.
Measuring the equilibrium relationships among urbanization, output growth, and environmental quality in Nigeria
Authors: Posu Sunday Mauton Ayodamola , Adeniyi Ademola Israel , Olubiyi Ebenezer Adesoji , Ojo Olutunji Timothy , Obisanya Adesile Adesegun
Published: October 2023

This study investigates the long-run relationship and short-run dynamics among urbanization, output growth, and environmental quality in Nigeria. The study adopts autoregressive distributed lag, bounds test co-integration analysis and the autoregressive integrated moving average with the annual time series dataset for 1990-2021. A long-run equilibrium relationship among urbanization, output growth, and environmental quality is established. Urban population, output growth, per capita GDP, and population growth are all significantly related to CO2 emissions. The study confirms the Environmental Kuznets Curve (EKC) hypothesis on per capita GDP and CO2 emissions in Nigeria. The positive interactive effect of urban population and output growth reduces CO2 emissions just as an increase in per capita GDP in a previous period produces a short-run effect of reducing CO2 emissions. The first-period lagged change in per capita GDP reports a marginal statistically significant negative effect on current CO2 emissions. The study utilizes data on past CO2 emissions to predict how CO2 emissions might evolve in the coming years and to examine the environmental sustainability of Nigeria. The forecast of CO2 emissions for 2023-2030 are relatively stable with some marginal fluctuations, which confirm the validated EKC. To sustain the validated inverted U-shaped EKC and to avoid it assuming an N-shape in the future, this study recommends environmental policy focus to monitor the growth of large cities and to implement measures capable of mitigating CO2 emissions by decoupling economic growth from CO2 emissions; and by encouraging investment in clean energy sources, sustainable transportation, and industrial practices.
JEL ClassificationE23 . O44 . O33 . Q28 . Q56 . R11
Phenotyping wild potatoes for photosynthesis associated traits under heat stress
Authors: Bashir Ikram , Nicolao Rodrigo , Haerter Janni André , de Brito Giovani Greigh , Castro Caroline , Heiden Gustavo
Published: October 2023

Heat stress is one of the major factors reducing potato yield by limiting the plant photosynthesis activity, production and partitioning of assimilates to the sink. Potato wild relatives (Solanum sect. Petota, Solanaceae) are a natural reservoir of valuable traits for abiotic stress resistance. However, it is necessary to assess the response of wild potato genotypes based on physiological features such as gas exchange, chlorophyll index and chlorophyll fluorescence that are promising candidates to improve crop photosynthesis under adverse conditions. Multivariate approaches on two factor experimental design were used to phenotype the best performing genotypes for most important traits under control and heat stress treatments. From the results, the most important traits to be focused on are net photosynthetic rates, transpiration rates, and stomatal conductance along with effective photochemical yield of PSII, Coefficient of photochemical yield of PSII and dry matter content, which are highly associated with heat stress conditions. Solanum commersonii accession (BGB451) and S. chacoense (BGB467, BGB113, BGB444, BGB083) are the top five genotypes of the heat stress tolerant group. However, BRSBEL, BGB003, BGB091 and BGB101 are most susceptible to heat stress. From our study, we concluded that most genotypes phenotyped for heat stress resistance belong to S. chacoense. The assessment of wild accessions for photosynthetic traits under heat stress is a reliable way to discriminate the most promising sources of abiotic stress resistance even though they do not have best quality tuber traits for the breeding of the potato crop.
Vacuum Sealing Drainage against surgical site infection after intracranial neurosurgery: a technical note
Authors: Ren Sen , Luo Yun , Shen Xiaoyong , Wu Qian , Wu Xiaohui , Ma Chao , Xiong Zhongwei , Gong Rui , Liu Zheng , chen jincao , Wang Wei
Published: October 2023

Background: Surgical site infections (SSIs)are still a challenge to neurosurgeons. However, Vacuum Sealing Drainage (VSD), which has been used extensively in the treatment of various infections after surgery, looks a promising solution. This study examines the efficacy and outcome of VSD treatment of pyogenic SSIs following intracranial neurosurgery.
Methods: 20 patients with infections who underwent surgical intervention were treated retrospectively using VSD at the Zhongnan hospital of Wuhan University over the past five years. Primary surgery types, SSI types, VSD replacements, surgical procedures, pathogenic germs, antibiotic therapy and infection control were reviewed and discussed.
Results: Of the 20 infections, 13 (65%) were extradural, and 7 (35%) were extradural SSIs combined with intracranial infections (including 5 meningitis, 1 subdural abscess, and 1 brain abscess). All the patients consented to medical device implantation (including 5 titanium webs, 6 bone flap fixation devices and 12 dura-plasties), with most of the devices removed during debridement. The median duration from primary surgery to a SSI diagnosis was 19 days (range: 7 to 365 days). All the patients also agreed to debridement and VSD treatment; VSD was replaced 0 to 5 times (median, one time) every 4 to 7 days and retained for 4 to 35 days (median, 14 days). Seven (35%) patients had defined bacterial infections, with staphylococcus aureus the dominant infection. The deployed standard VSD and antibiotic treatment ensured full recovery from SSIs, including from intracranial infections: 14 (70%) patients were free of infection during follow-up, and no infection-associated death was registered; 6 (30%) patients died of severe primary affections.
Conclusion: VSD-assisted therapy is safe and effective against SSIs after intracranial neurosurgery.
The Effect of Floating Chamber Design on Co2 Flux Measurements
Authors: Lee Daryl Jia Jun , ZHAN Li Yang , WONG Wei Wen , POH Seng-Chee
Published: October 2023

The floating chamber is valued for its simplicity, affordability, and adaptability for widespread use in measuring diffusive greenhouse gases flux from surface water, although its effectiveness may vary based on its chamber design. This study investigates the effect of chamber insulation on gas flux accumulation. Our findings reveal that non-insulated chambers experienced greater solar heating effects in their headspace, with a 20% deviation from the targetted water temperature. In contrast, insulated chambers showed less than 1% temperature difference with ambient condition. Solar-induced heating of the chamber walls increased the internal temperature, leading to an overestimation of greenhouse gas fluxes. The developed floating chambers were tested at a hydropower reservoir in Terengganu, Malaysia. Results from two distinct seasonal sampling campaigns at three sites demonstrate that CO2 fluxes during the wet season (45 mmol m-2d-1) were almost three times higher compared to the dry season (16 mmol m-2d-1).
The Clinical survival benefit of postmastectomy radiotherapy patients screened from T1-2N1M0 breast cancer according to tumor size and the number of lymph nodes
Authors: Wu Nisha , Li Junyan , Zhang Hongying , Su Xiaohan , Tan Qiao , Hou Lingmi , Chen Maoshan
Published: October 2023

Purpose
our study was aimed at systematically evaluated the advantage of the treatment of postmastectomy radiotherapy (PMRT) on the overall survival (OS) depending on the lymph nodes number and the size of tumors in patients who had breast cancer in stage of T1-2N1.
Results
There were 29,366 patients included in the last analysis, involving 20,167 (68.7%) cases in the group without PMRT and 9199 (31.3%) cases in the group with PMRT. Multivariable Cox model analysis presented that the treatment of radiotherapy could improve the OS (Hazard ratio HR, 0.89;95%CI 0.83–0.96, P = 0.001)in patients who had breast cancer in stage of T1-2N1M0. The analysis of subgroups according to different categories of tumor burden showed that PMRT did not provide an overall survival advantage in tumor size and lymph node subgroups. The advantages of PMRT are related with an increase in overall survival rate in patients who had tumors 4–5 cm in size and 2 or 3 positive lymph nodes (HR 0.69; 95%CI 0.51–0.94; P = 0.017or HR 0.65; 95%CI 0.44–0.95; P = 0.027, respectively). Patients who had tumors size 2–3 cm and 3 positive lymph nodes benefited from PMRT as well (HR 0.61; 95%CI 0.48–0.78; P < 0.001), but other subgroups of patients did not benefit from PMRT .
Conclusion
The size of breast cancer tumors and positive nodes number appear to be receivable forecast of overall survival benefits after the treatment of radiotherapy, which may allow clinicians to carry out more accurate and effective PMRT decisions in the clinic, especially for the subgroup who had the low-risk breast cancer.
Methods
Using data from the SEER program from 2004 to 2015, we calculated the use of PMRT and survival in patients with breast cancer who were in stage of T1-T2 and had 1–3 positive lymph nodes that were diagnosed with invasive breast cancer and underwent mastectomy. Patients were selected according to whether they received postoperative radiotherapy. And 29366 matched patients enrolled in the final analysis were respectively included in the post-mastectomy radiotherapy group (PMRT) and non-radiotherapy group (no-PMRT). We analyzed patients in subgroup analysis who had different positive nodes number and the size of tumors. Overall survival (OS) was calculated with the Kaplan-Meier method in comparison with the survival between different subgroups. We carried out Multivariate Cox proportional hazard models to evaluate the analysis of factors on prognosis and estimated the hazard ratios (HR) and their 95% confidence intervals (CI).
Repeated exposure to the synthetic cannabinoid JWH-018 induces enduring immune and glial alterations in the rat brain
Authors: Pintori Nicholas , Mostallino Rafaela , Orrù Valeria , Piras Maria Grazia , Castelli M. Paola , Luca Maria Antonietta
Published: October 2023

Background
The misuse of synthetic cannabinoid receptor agonists (SCRAs) poses major psychiatric risks. We previously showed that repeated exposure to the prototypical SCRA JWH-018 induces alterations in dopamine (DA) transmission, abnormalities in the emotional state, and glial cell activation in the mesocorticolimbic DA circuits of rats. Despite growing evidence suggesting the relationship between drugs of abuse and neuroinflammation, little is known about the impact of SCRA on the neuroimmune system. Here, we investigated whether repeated JWH-018 exposure altered neuroimmune signaling, which could be correlated with previously reported central effects.
Methods
Adult male Sprague‒Dawley rats were exposed to JWH-018 (0.25 mg/kg, i.p.) for fourteen consecutive days, and the expression of cytokines, chemokines, and growth factors was measured seven days after treatment discontinuation in the striatum, cortex, and hippocampus. Moreover, microglial (ionized calcium binding adaptor molecule 1, IBA-1) and astrocyte (glial fibrillary acidic protein, GFAP) activation markers were evaluated in the caudate-putamen (CPu).
Results
Repeated JWH-018 exposure induces a perturbation of neuroimmune signaling specifically in the striatum, as shown by increased levels of cytokines [interleukins (IL) -2, -4, -12p70, -13, interferon (IFN) γ], chemokines [macrophage inflammatory protein (MIP) -1α, -3α], and growth factors [macrophage colony-stimulating factor (M-CSF), vascular endothelial growth factor (VEGF)], together with increased IBA-1 and GFAP expression in the CPu.
Conclusions
JWH-018 exposure induces enduring brain region-specific immune alterations, which may contribute to the behavioral and neurochemical dysregulations in striatal areas that play a role in reward and reward-related processes, such as addictive behaviors.
Detection of low expression of molecular subtype HER2 in breast cancer using ultrasound-based radiomics
Authors: Zhang Xueling , Wu Shaoyou , Zu Xiao , Li Xiaojing , Zhang Qing , Ren Yongzhen , Tong Shan , Li Hongbo
Published: October 2023

Purpose: HER2-low expression breast cancer (BC) accounts for nearly half of all breast cancers which may benefit from new antibody-drug conjugates targeted treatments. HER2-low BC is gradually being recognized as a distinct subtype. Therefore, we aimed to use ultrasound-based radiomics（USR）to develop an efficient evaluation approach of HER2-low status.
Methods: 222 patients with a histologically diagnosis of BC were retrospectively analyzed and randomly divided into training and test cohort. Radiomics features were extracted from the preoperative ultrasound images, followed by Lasso regression for dimension reduction.Based on the selected features, the optimal machine learning classifier was selected to construct a USR model to predict HER2-low expression. Multivariable logistic regression was used to identify independent clinical risk factors.Finally, a clinical-USR model incorporating the radiomics features and the clinical risk factors was constructed. Model performance was assessed using receiver operating characteristic curve and decision curve analysis.
Results: The USR model and clinical-USR model had good predictive ability in the training cohort ( AUC 0.91; 0.86 , respectively) and test cohort ( AUC 0.83; 0.78 , respectively).Both of them outperformed the clinical model (p < 0.05, DeLong test).Decision curve analysis confirmed that the model had clinical utility.
Conclusion: The machine learning model based on ultrasound images had high prediction value for HER2-low BC.