bluuebunny/crossref_metadata_2025 huggingface dataset:
SARS-CoV-2 vaccine excipients polyethylene glycol and trometamol do not induce mast cell degranulation, in an in vitro model for non-IgE-mediated hypersensitivity
Authors: Quan Paola Leonor , Ollé Laia , Sabaté-Brescó Marina , Guo Yanru , Muñoz-Cano Rosa , Wagner Annette , Gastaminza Gabriel , Martín Margarita
Published: December 2022

The development of vaccines against SARS-CoV2 brought about several challenges, including the management of hypersensitivity reactions to these formulations. The search for underlying mechanisms involved in these adverse events initially focused on excipients which may trigger mast cell activation responses via non-IgE pathways: polyethylene glycol and trometamol. We sought to determine whether these components, in their pure form, were capable of stimulating mast cells directly. To test this hypothesis, we used an in vitro model for non-IgE-mediated activation that has previously shown degranulation responses induced via MRGPRX2 with known drug agonists of the receptor. Human LAD2 mast cells were incubated with different concentrations (1, 10, 50 mg/ml) of trometamol and of purified polyethylene glycol/Macrogol (molecular weights: 2,000, 3,350, 4,000, and 6,000). Mast cell degranulation was assessed using a beta-hexosaminidase read-out. Interestingly, degranulation responses for all reagents tested showed no significant differences from those obtained from the negative control (basal degranulation). Receptor-silencing assays were therefore not conducted. In summary, purified PEG and trometamol did not induce mast cell degranulation in this in vitro model for the study of non-IgE mechanisms of drug hypersensitivity, previously shown to be useful in the investigation of MRGPRX2 ligands. Studies using complete vaccine formulations, lipid conjugates, and receptor gene variants are needed to further clarify mechanisms of vaccine hypersensitivity.
Exosomes based advancements for application in medical aesthetics
Authors: Zhang Bin , Gong Jianmin , He Lei , Khan Adeel , Xiong Tao , Shen Han , Li Zhiyang
Published: December 2022

Beauty is an eternal pursuit of all people. Wound repair, anti-aging, inhibiting hyperpigmentation and hair loss are the main demands for medical aesthetics. At present, the repair and remodeling of human body shape and function in medical aesthetics are often achieved by injection of antioxidants, hyaluronic acid and botulinum toxin, stem cell therapy. However, there are some challenges, such as difficulty controlling the injection dose, abnormal local contour, increased foreign body sensation, and the risk of tumor occurrence and deformity induced by stem cell therapy. Exosomes are tiny vesicles secreted by cells, which are rich in proteins, nucleic acids and other bioactive molecules. They have the characteristics of low immunogenicity and strong tissue penetration, making them ideal for applications in medical aesthetics. However, their low yield, strong heterogeneity, and long-term preservation still hinder their application in medical aesthetics. In this review, we summarize the mechanism of action, administration methods, engineered production and preservation technologies for exosomes in medical aesthetics in recent years to further promote their research and industrialization in the field of medical aesthetics.
Functional dissection of N-terminal nuclear trafficking signals of SETDB1
Authors: Eom Jaemin , Jeon Kyuheum , Park Jung Sun , Kang Yong-Kook
Published: December 2022

SETDB1 is a histone H3-lysine 9-specific methyltransferase that fulfills epigenetic functions inside the nucleus; however, when overexpressed, SETDB1 majorily localizes in the cytoplasm. SETDB1 has a single nuclear-localization-signal (NLS) motif and two successive nuclear-export-signal (NES1 and NES2) motifs in the N-terminus, suggesting that SETDB1 localization is the consequence of a balance between the two antithetic motifs. Here, we performed a series of motif deletions to characterize their effects on the cellular movement of SETDB1. Given the cytoplasmic localization of GFP-SETDB1 in the whole form, without the NES motifs, GFP-SETDB1 was not nuclear, and 3xNLS addition plus NES removal held the majority of GFP-SETDB1 within the nucleus. The results indicated that the cytoplasmic localization of GFP-SETDB1 is the combined result of weak NLS and robust NESs. In ATF7IP-overexpressing cells, GFP-SETDB1 entered the nucleus only in the presence of the NES1 motif; neither the NES2 nor NLS motif was necessary. Since subcellular fractionation results showed that ATF7IP was nuclear-only, an intermediary protein may interact specifically with the NES1 motif after stimulation by ATF7IP. When GFP-SETDB1 had either NES1 or NES2, it was precipitated (in immunoprecipitation) and colocalized (in immunofluorescence) with ATF7IP, indicating that GFP-SETDB1 interacts with ATF7IP through the NES motifs in the nucleus. The regulated nuclear entry of SETDB1 is assumed to set a tight restriction on its abundance within the nucleus, thereby ensuring balanced nuclear SETDB1 levels.
Moving through a changing world: Single cell migration in 2D vs. 3D
Authors: Pawluchin Anna , Galic Milos
Published: December 2022

Migration of single adherent cells is frequently observed in the developing and adult organism and has been the subject of many studies. Yet, while elegant work has elucidated molecular and mechanical cues affecting motion dynamics on a flat surface, it remains less clear how cells migrate in a 3D setting. In this review, we explore the changing parameters encountered by cells navigating through a 3D microenvironment compared to cells crawling on top of a 2D surface, and how these differences alter subcellular structures required for propulsion. We further discuss how such changes at the micro-scale impact motion pattern at the macro-scale.
Immune activation of characteristic gut mycobiota Kazachstania pintolopesii on IL-23/IL-17R signaling in ankylosing spondylitis
Authors: Zhang Haiting , Wei Yu , Jia Huanhuan , Chen Diling , Tang Xiaocui , Wang Jian , Chen Meili , Guo Yinrui
Published: December 2022

It is very important to understand the communication and interaction mechanisms between the host and its resident microorganisms on host physiology and for precise diagnosis and treatment. Although intestinal fungi and bacteria dysbiosis is increasingly linked to ankylosing spondylitis (AS), their mechanisms of action have been rarely illustrated. In this paper, fecal samples from 10 AS monkeys and 10 healthy controls were collected to systematically characterize the gut mycobiota and microbiota in AS monkeys by 16S rRNA and ITS2 DNA sequencing. Our results showed the gut fungi of Kazachstania pintolopesii, Saccharomycetaceae, Kazachstania, and Saccharomyceteles. Saccharomycetes were specially enriched in AS, and the microbiota of AS monkeys was characterized by an increased abundance of Clostridia, Clostridiales, Ruminococcaceae, and Prevotella 2, using Line Discriminant Analysis Effect Size. Compared to healthy controls, decreased ITS2/16S biodiversity ratios and altered bacterial–fungal interkingdom networks were observed in AS monkeys. Oral administration of K. pintolopesii activates IL-17RA pathway and induce inflammatory reaction in the colonic tissue of C57BL/6 mice, as well as multiple AS phenotypes, including fungal and bacterial dysbiosis, immune responses of NK cells, platelets, T cells, leukocytes, B-cell activation, rheumatoid arthritis, and inflammatory bowel disease. We also found the secreted products of K. pintolopesii could activate the IL-17RA pathway, which induces PANoptosis in macrophage RAW264.7 cells. Much worse, the PANoptosis products could promote the proliferation and morphological changes of K. pintolopesii, which resulted in much more K. pintolopesii and a severe inflammatory reaction. Interestingly, the inflammatory factor TNF-α can promote the morphological transformation of Candida albicans and K. pintolopesii, which is worthy of further study. The characteristic fungi in all these findings implied that fungal and bacterial dysbiosis have a close link to AS and that their communication and interaction indeed play an important role in autoimmune responses, and K. pintolopesii could be a potential marker microorganism in AS, although its specific mechanism is not fully elucidated.
Introducing IPOACHED: A conservation criminology-based framework to understand wildlife species targeted by poachers in protected areas
Authors: Kahler Jessica S. , Rivera Christian J. , Gore Meredith L.
Published: December 2022

The criminogenic dimensions of conservation are highly relevant to contemporary protected area management. Research on crime target suitability in the field of criminology has built new understanding regarding how the characteristics of the crime targets affect their suitability for being targeted by offenders. In the last decade, criminologists have sought to apply and adapt target suitability frameworks to explain wildlife related crimes. This study seeks to build upon the extant knowledge base and advance adaptation and application of target suitability research. First, we drew on research, fieldwork, and empirical evidence from conservation science to develop a poaching-stage model with a focus on live specimens or wild animals- rather than a market stage and wildlife product-focused target suitability model. Second, we collected data in the Intensive Protection Zone of Bukit Barisan Selatan National Park (BBSNP), Sumatra, Indonesia through surveys with local community members (n=400), and a three-day focus group with conservation practitioners (n= 25). Our target suitability model, IPOACHED, predicts that species that are in-demand, passive, obtainable, all-purpose, conflict-prone, hideable, extractable, and disposable are more suitable species for poaching and therefore more vulnerable. When applying our IPOACHED model, we find that the most common response to species characteristics that drive poaching in BBSNP was that they are in-demand, with support for cultural or symbolic value (n=101 of respondents, 25%), ecological value (n=164, 35%), and economic value (n=234, 59%). There was moderate support for the conflict-prone dimension of the IPOACHED model (n=70, 18%). Other factors, such as a species lack of passiveness, obtainability and extractability, hamper poaching regardless of value. Our model serves as an explanatory or predictive tool for understanding poaching within a conservation-based management unit (e.g., a protected area) rather than for a specific use market (e.g., pets). Conservation researchers and practitioners can use and adapt our model and survey instruments to help explain and predict poaching of species through the integration of knowledge and opinions from local communities and conservation professionals, with the ultimate goal of preventing wildlife poaching.
Evaluation of risk stratification program based on trajectories of functional capacity in patients with acute coronary syndrome: The REACP study
Authors: Li Dongze , Chen Xiaoli , Li Fanghui , Jia Yu , Li Zhilin , Liu Yi , Ye Lei , Gao Yongli , Zhang Wei , Li Hong , Zeng Rui , Wan Zhi , Zeng Zhi , Cao Yu
Published: December 2022

Background

As a validated assessment tool for functional disability (activities of daily living), the Barthel index (BI) assessed initially at admission has the potential to stratify patients with high-risk acute coronary syndrome (ACS). Dynamic trajectory evaluation of functional capacity in hospitals may provide more prognostic information. We aimed to establish a novel dynamic BI-based risk stratification program (DBRP) during hospitalization to predict outcomes among ACS patients.

Methods

A total of 2,837 ACS patients were included from the Retrospective Multicenter Study for Early Evaluation of Acute Chest Pain. The DBRP rating (low, medium, and high-risk categories) was calculated from dynamic BI at admission and discharge. The primary outcome was all-cause mortality, and the secondary outcome was cardiac mortality.

Results

Of all the included patients, 312 (11%) died during a median follow-up period of 18.0 months. Kaplan–Meier analysis revealed that the cumulative mortality was significantly higher in patients in the higher risk category according to the DBRP. Multivariable Cox regression analysis indicated that, compared to the low-risk category, the higher risk category in the DBRP was an independent strong predictor of all-cause mortality after adjusting for confounding factors (medium-risk category: hazard ratio [HR]: 1.756, 95% confidence interval [95% CI]: 1.214–2.540; P = 0.003; high-risk category: HR: 5.052, 95% CI: 3.744–6.817; P < 0.001), and the same result was found for cardiac mortality.

Conclusion

The DBRP was a useful risk stratification tool for the early dynamic assessment of patients with ACS.

Clinical trial registration

[http://www.chictr.org.cn], identifier [ChiCTR1900024657].
Severe early-onset manifestations of generalized arterial calcification of infancy (mimicking severe coarctation of the aorta) with ABCC6 gene variant — Case report and literature review
Authors: Fãgãrãşan Amalia , Gozar Liliana , Ghiragosian Simina-Elena Rusu , Murariu Mircea , Pop Marian , Crauciuc Andrei , Miclea Diana , Şuteu Carmen Corina
Published: December 2022

Introduction

Generalized arterial calcification of infancy (GACI) is a rare cause of infantile heart failure and systemic hypertension with a poor prognosis, characterized by extensive calcification and proliferation of the intimal layer of large and medium sized arteries.

Case report

We present the first case report of successful surgical treatment of severe aortic arch obstruction by calcified plaques mimicking severe coarctation of the aorta and the outcome (of bisphosphonate therapy) in a newborn with GACI. Furthermore, we report the identification of a variant in ATP Binding Cassette Subfamily C, Member 6 (ABCC6) gene, possibly associated with severe early-onset manifestations of GACI.

Conclusion

This case report highlights the importance of considering GACI in an infant with heart failure, systemic hypertension, and evidence of increased echogenicity of the arterial vessels. We noted the favorable outcome in improving the aortic calcification in our patient after surgical treatment and bisphosphonates therapy. Early diagnosis and treatment improve the long-term prognosis. A better understanding of this rare genetic disease could lead to new therapeutic strategies.
Loss of perceived social role, an index of social frailty, is an independent predictor of future adverse events in hospitalized patients with heart failure
Authors: Watanabe Ayako , Katano Satoshi , Yano Toshiyuki , Nagaoka Ryohei , Numazawa Ryo , Honma Suguru , Yamano Kotaro , Fujisawa Yusuke , Ohori Katsuhiko , Kouzu Hidemichi , Ishigo Tomoyuki , Katayose Masaki , Hashimoto Akiyoshi , Furuhashi Masato
Published: December 2022

Aims

Although the impact of physical frailty on prognosis and the effect of cardiac rehabilitation in HF patients has been well established, data for the prognostic impact of social frailty (SF) in HF patients are limited. In addition, the relative importance of each SF domain in clinical outcomes remains unclear. We aimed to get a new insight into the associations of SF with clinical outcomes in elderly hospitalized HF patients.

Methods

A single-center, retrospective cohort study was conducted using data from 310 in-hospital HF patients aged ≥ 65 years (mean age of 78 ± 8 years; 49% women). Makizako’s five questions, a self-reported questionnaire, were used to define SF. The primary outcome was composite events defined by all-cause death and cardiovascular events.

Results

Of the 310 elderly HF patients, 188 patients (61%) had SF. Seventy-five patients (24%) had composite events during a mean follow-up period of 1.93 ± 0.91 years. Kaplan-Meier curves showed that patients with SF had a significantly higher composite event rate than patients without SF. In multivariate Cox regression analyses, SF was independently associated with a higher composite event rate after adjusting for prognostic markers [adjusted hazard ratio (HR), 2.01; 95% confidence interval (CI), 1.07–3.78; p = 0.04]. Of the 5 questions for defining SF, an answer of yes to the question about not feeling helpful toward friends or family, which indicates loss of perceived social role, was an independent predictor of composite events (adjusted HR, 2.28; 95% CI, 1.36–3.82; p < 0.01). Inclusion of loss of perceived social role into the baseline prognostic model improved both the continuous net reclassification improvement (0.562; 95% CI, 0.298–0.827; p < 0.01) and integrated discrimination improvement (0.031; 95% CI, 0.006–0.056; p = 0.02).

Conclusion

Loss of perceived social role is associated with increased adverse event risk and provides additive prognostic information in elderly HF patients.
XMA2: A crossbar-aware multi-task adaption framework via 2-tier masks
Authors: Zhang Fan , Yang Li , Meng Jian , Seo Jae-sun , Cao Yu , Fan Deliang
Published: December 2022

Recently, ReRAM crossbar-based deep neural network (DNN) accelerator has been widely investigated. However, most prior works focus on single-task inference due to the high energy consumption of weight reprogramming and ReRAM cells’ low endurance issue. Adapting the ReRAM crossbar-based DNN accelerator for multiple tasks has not been fully explored. In this study, we propose XMA2, a novel crossbar-aware learning method with a 2-tier masking technique to efficiently adapt a DNN backbone model deployed in the ReRAM crossbar for new task learning. During the XMA2-based multi-task adaption (MTA), the tier-1 ReRAM crossbar-based processing-element- (PE-) wise mask is first learned to identify the most critical PEs to be reprogrammed for essential new features of the new task. Subsequently, the tier-2 crossbar column-wise mask is applied within the rest of the weight-frozen PEs to learn a hardware-friendly and column-wise scaling factor for new task learning without modifying the weight values. With such crossbar-aware design innovations, we could implement the required masking operation in an existing crossbar-based convolution engine with minimal hardware/memory overhead to adapt to a new task. The extensive experimental results show that compared with other state-of-the-art multiple-task adaption methods, XMA2 achieves the highest accuracy on all popular multi-task learning datasets.
Role of aerobic exercise in ameliorating NASH: Insights into the hepatic thyroid hormone signaling and circulating thyroid hormones
Authors: Liu Qiuhong , Li Han , He Weiwei , Zhao Qing , Huang Caoxin , Wang Qingxuan , Zheng Zeyu , Zhang Xiaofang , Shi Xiulin , Li Xuejun
Published: December 2022

Aim

Triiodothyronine (T3) administration significantly eliminates hepatic steatosis and also has a therapeutic effect on non-alcoholic steatohepatitis (NASH). However, the potential mechanism by which T3-mediated exercise improves NASH is unknow. This study aimed to explore the effect of aerobic exercise on liver injury in NASH.

Methods

Aerobic exercise was conducted to explore the effects of exercise on liver injury in NASH model induced by Atherosclerotic (Ath) diet. Biochemical evaluations, histological staining and real-time PCR were first applied to confirm the amelioration effects of exercise on NASH. RNA-sequencing (RNA-seq) analysis for livers of each group were further used to identify the underlying mechanisms of aerobic exercise. Bioinformatics methods were used to explore the key functional pathways involved in the improvement of liver tissue in NASH mice by aerobic exercise.

Results

Aerobic exercise improved hepatic steatosis, lobular inflammation and fibrosis in NASH mice. multiple inflammation-related pathways were significantly enriched in the liver of NASH group and improved by aerobic exercise. The results of gene set variation analysis (GSVA) showed a higher enrichment score of T3 response signature in NASH mice with exercise. Increased Dio1 expression in the liver of NASH with exercise mice and increased circulating FT3 and FT4 levels upon aerobic exercise were confirmed.

Conclusions

We found that aerobic exercise could significantly reduce hepatic lipid accumulation, inflammatory infiltration and fibrosis progression in the liver of NASH mice. Hepatic thyroid hormone signaling activation and circulating thyroid hormones is potentially involved in the amelioration effect of aerobatic exercise on NASH progression.
Retroperitoneal laparoscopic partial adrenalectomy (RLPA) for 20-40 mm nonfunctional adrenal tumors in the day surgery mode
Authors: Li Xuwen , Xi Haibo , Yu Yue , Liu Wei , Zhu Xiaoping , Gong Zhixian , Fu Bin , Wang Gongxian , Zhou Xiaochen
Published: December 2022

Objectives

To investigate the outcome and safety of retroperitoneal laparoscopic partial adrenalectomy in the treatment of nonfunctional unilateral adrenal tumors in the day surgery mode.

Methods

Nineteen patients bearing nonfunctional unilateral 20-40 mm adrenal tumors were prospectively enrolled and underwent retroperitoneal laparoscopic partial adrenalectomy in the day surgery unit of our hospital between June 2021 and March 2022. All patients were diagnosed with non-functional adrenal tumors as outpatients before being admitted to the day surgery unit with their consent. Patient demographics and perioperative data were prospectively documented. The patients were followed up by telephone on day 1, 3 and 7 after discharge and followed up for 6 months.

Results

The patient’s age was 50.5 ± 11.9 yr (range from 19.0 - 69.0). Seven patients were female. Twelve patients underwent surgery on the left side. The maximal diameter of tumor was 28.3 ± 5.7 mm (20.0 - 40.0 mm). Operation time was 72.1 ± 14.9 min (58.0 - 120.0 min). Mean blood loss was 64.7 ± 50.4 ml (30.0 - 200.0 ml). The gastrointestinal function recovery time was 9.7 ± 2.6 h (6.0 - 16.0 h). Retroperitoneal drainage was removed 24.8 ± 13.3 h (range 18.0 - 72.0) after surgery. Four patients were transferred to the general ward for postoperative management, while others were discharged within 24 hours after surgery. Length of hospital stay was 48.8 ± 13.1 h (38.0 - 85.0h). Hospitalization expense was 24168.4 ± 2910.3 RMB¥ (20844.3 - 34369.8 RMB¥). Postoperative pathology revealed 17 cortical adenoma, 1 pheochromocytoma and 1 lymphatic duct tumor.

Conclusion

Retroperitoneal laparoscopic partial adrenalectomy for nonfunctional unilateral adrenal tumors in the day surgery mode is safe when strict selection criteria and perioperative management protocol are followed, which has the potential to shorten length of hospital stay and reduce lower hospitalization costs.
Large extent of mercury stable isotope fractionation in contaminated stream sediments induced by changes of mercury binding forms
Authors: Schwab Lorenz , Rothe Florian M. , McLagan David S. , Alten Alexandra , Kraemer Stephan M. , Biester Harald , Wiederhold Jan G.
Published: December 2022

Mercury (Hg) release from contaminated legacy sites is a large contributor to riverine ecosystems and can represent a significant local and regional environmental issue even long after the initial site contamination. Understanding processes of in-stream species transformation is therefore important to assess the fate and bioavailability of the released Hg. In this study, we investigated in-stream Hg transformation processes with analyses of Hg binding forms and Hg stable isotopes. Stream sediments were collected downstream of a former kyanization facility (Black Forest, SW Germany), where highly soluble Hg(II)-chloride (HgCl2) was used as an anti-fouling agent to treat timber. Exfiltration of partly anoxic, contaminated groundwater with Hg concentrations of up to 700 μg L−1 into the adjacent Gutach stream is the main source of Hg to sediments. Total Hg concentrations in the stream bottom sediments (<2 mm) ranged from background values of 6.3 µg kg−1 upstream of the contaminated site to 77 mg kg−1 near the location of exfiltration of contaminated groundwater. A five-step sequential extraction procedure and Hg pyrolytic thermal desorption (PTD) analyses indicated changes in Hg binding forms in the sediments along the flow path towards a higher proportion of organically bound Hg. A large shift towards negative δ202Hg values was observed downstream of the contaminated site (change of ≈2‰) along with a minor offset in mass-independent fractionation. Binary mixing models based on Hg isotope ratios using one industrial and different natural background endmembers were tested to estimate their respective contribution of Hg to the sediments but failed to produce plausible allocations. Based on the observed changes in isotopic composition, total Hg concentrations and Hg binding forms, we propose that the large extent of fractionation observed in downstream sediments is the result of a combination of kinetic isotope effects during sorption, redistribution of Hg within the sediment and the preferential transport of Hg associated with the sediment fine fraction. These results highlight the importance of transformation processes when assessing the sources and fate of Hg in environmental systems and show limitations of using simple mixing models based on Hg stable isotopes.
Evaluating methods to map burned area at 30-meter resolution in forests and agricultural areas of Central India
Authors: Chandel Abhinav , Sarwat Wajida , Najah Abdul , Dhanagare Sujay , Agarwala Meghna
Published: December 2022

Biomass burning is a major phenomenon that plays an important role in small-scale ecological processes such as vegetation dynamics and soil erosion, and global processes such as hydrological cycles and climate change. However, global fire databases have low accuracies for burned area detection in areas with small fires, low biomass and in woodlands and open forests that characterize Central India. The present study uses higher resolution (30 meter) Landsat imagery to test accuracies for burned area detection using spectral indices (SI), machine learning (ML) algorithms and supervised classification. We find that detection of burned area by global fire product Fire Information for Resource Management System (FIRMS) is very low (<20%). Accuracies are higher for Landsat-based classification of burned area using supervised classification, random forest (RF) and Support Vector Machines (SVM). Accuracies are higher in April–May than in February–March and vary by azimuth angle on the day of image acquisition. RF produced the most consistently high classification accuracies for April (>80%), but had a tendency to misclassify less frequently available land covers; SVM had similar classification accuracies but had a tendency to overfit the model. Both lead to the potential for increasing commission errors and need to be used carefully when predicting burned area. Inclusion of SI had high relative importance in predicting burned area and reduced commission errors. Given these caveats, we recommend using ML algorithms for mapping burned area in the future, as it requires less time investment than classification and can yield consistent results. Accurate mapping of high-resolution fires is important for more accurate inputs into carbon inventories and ecological understanding of land-use dynamics and drivers.
Second-order Stokes wave-induced dynamic response and instantaneous liquefaction in a transversely isotropic and multilayered poroelastic seabed
Authors: Zhang Zhiqing , Zhou Bohao , Li Xibin , Wang Zhe
Published: December 2022

The ocean waves exhibit obvious non-linearity with asymmetric distribution of wave crests and troughs, which could induce significantly different effect on the seabed compared to the commonly used linear wave theory. In this paper, a semi-analytical solution for a transversely isotropic and multilayered poroelastic seabed under non-linear ocean wave is proposed by virtue of the dual variable and position (DVP) method. The ocean wave and seabed are, respectively, modelled using second-order Stokes theory and Biot’s complete poroelastodynamic theory. Then the established governing equations are decoupled and solved via the powerful scalar potential functions. Making use of the DVP scheme, the layered solutions are finally gained by combining the boundary conditions of the seabed. The developed solutions are verified by comparing with existing solutions. The selected numerical examples are presented to investigate the effect of main parameters on the dynamic response of the seabed and evaluate the corresponding liquefaction potential. The results show that the anisotropic stiffness and permeability, degree of saturation and stratification have remarkable influence on the dynamic response and liquefaction behavior of the seabed. The present solution is a useful tool to estimate the stability of transversely isotropic and layered seabed sediment in the range of non-linear ocean wave.
Refined numerical study on the effect of grouting plumpness on bearing behavior of pipe roof
Authors: Shi Yufeng , Hu Menghao , Zhang Tao , Chen Xiangsheng , Zhou Yuhang , Min Shichao , Jiang Yalong
Published: December 2022

Pre-supported pipe roof grouting is difficult to be satisfied due to various influences, which may have an impact on its bearing characteristics. In order to determine the impact of grouting plumpness on the bearing behavior of pipe roof, based on the preliminary experimental research a refined numerical model that takes into account the bonding between the steel pipe and cement mortar was established in this paper. It was used to analyze the bending moment-deflection curve, variation laws of ultimate bearing capacity coefficient, and disengagement rate of pipe roof under different grouting plumpness. Meanwhile, the working conditions of reinforcement and casing in the pipe were conducted. The results show that compared with full grouting, the ultimate bearing capacity of the grouting pipe roof, pipe roof with reinforcement bundles and casing pipe decreases by 4.6%, 5.2%, and 4.8% respectively when grouting incompleteness is 2%, and decreases by 35.3%, 40.4%, and 37.1% respectively, when grouting incompleteness rises to 25%; The ultimate bearing capacity of the pipe roof can be greatly enhanced by adding reinforcement bundles and casing pipe, and the increases of this case is 7.7%–18.3%, 31.4%–51.6%, respectively; The disengagement rate of pipe roof and mortar under the same load increases with the increase of grouting incompleteness, which growing relatively slowly when the grouting incompleteness is less than 2%, and develops rapidly when it is 2%–30%, while growing little when it is more than 30%. Under the same grouting incompleteness, adding reinforcement bundles and casing pipe in the pipe roof can effectively reduce its debonding rate and the latter has a better effect, and the corresponding disengagement rate is 62.8%–97.9% and 29.1%–91.9% of the grouting pipe roof respectively.
Coagulation status and determinants of possible aspirin resistance in patients with essential thrombocythemia
Authors: Yang Erpeng , Lv Yan , Wang Ziqing , Wang Dehao , Li Yumeng , Sun Yan , Zhang Yanyu , Niu Jicong , Chen Zhuo , Liu Weiyi , Hu Xiaomei
Published: December 2022

Objectives

The currently recommended aspirin regimen appears inadequate for thromboprophylaxis in essential thrombocythemia (ET). This study aimed not only to evaluate the curative effect of aspirin but also to explore the coagulation status and determinants of aspirin resistance (AR) of ET patients.

Methods

A total of 80 ET patients who underwent coagulation tests, thromboelastography (TEG), and next-generation sequencing (NGS) were involved in the study. Patients were divided into the aspirin sensitivity (AS) group and AR group according to the arachidonic acid inhibition rate. Their clinical features and coagulation function were analyzed.

Results

The incidence of AR was 53.75% (43/80) in 80 ET patients. Fbg was significantly higher in coagulation tests in AR patients compared with AS patients (P < 0.05), while the differences in other variables (D-D, PT, PTA, INR, APTT, TT, FDP, and AT-III) were not statistically significant (P > 0.05). Compared with AS patients, the K values, α angles, MA values, and CI values of TEG in AR patients were statistically smaller (P < 0.05), but there was no significant difference in R value between them (P > 0.05). Univariate and multivariate logistic regression analysis showed that age, irregular use of aspirin, smoking, dyslipidemia, and hypertension increased the risk of AR (P < 0.05). In the routine NGS, the driver gene and non-driver gene had no effect on AR in ET patients.

Conclusion

Compared with AS patients, AR patients have enhanced platelet aggregation function, are in a relatively hypercoagulable state, and haveelevated fibrinogen function/levels, all of which cause a worse coagulation status. ET patients with increasing age, irregular use of aspirin, smoking, dyslipidemia, and hypertension are possibly at higher risk of AR. The routine NGS may not be helpful for the prediction of AR, therefore we recommend adding relevant drug-resistance genes to NGS.
EQ-5D full health state after therapy heralds reduced hazard to accrue subsequent organ damage in systemic lupus erythematosus
Authors: Lindblom Julius , Zetterberg Sture , Emamikia Sharzad , Borg Alexander , von Perner Gunilla , Enman Yvonne , Heintz Emelie , Regardt Malin , Grannas David , Gomez Alvaro , Parodis Ioannis
Published: December 2022

Objectives

To investigate whether self-reported EQ-5D full health state (FHS) after therapeutic intervention for active systemic lupus erythematosus (SLE) is associated with a reduced risk to accrue organ damage. In a separate analysis, we sought to investigate associations between experience of “no problems” in each one of the five dimensions of EQ-5D and the risk to accrue damage.

Methods

Data from the open-label extension periods of the BLISS-52 and BLISS-76 trials of belimumab in SLE (NCT00724867; NCT00712933) were used (N = 973). FHS was defined as an experience of “no problems” in all five EQ-5D dimensions. Organ damage was assessed annually using the Systemic Lupus International Collaborating Clinics (SLICC)/American College of Rheumatology (ACR) Damage Index (SDI). Associations between the three-level version of the EQ-5D (EQ-5D-3L) responses at open-label baseline and the first documented increase in organ damage were investigated using Cox regression accounting for age, sex, ancestry, SDI at baseline, and background therapy, and associations with SDI items were investigated using phi (φ) correlation analyses.

Results

A total of 147 patients (15.1%) accrued organ damage during follow-up, with the first increase in their SDI score occurring after a mean time of 29.1 ± 19.6 months. Lower proportions of FHS respondents accrued damage over a course of up to 7.9 years of open-label follow-up compared with no FHS respondents (p = 0.004; derived from the logrank test). FHS was associated with a reduced hazard to accrue subsequent organ damage (HR: 0.60; 95% CI: 0.38–0.96; p = 0.033) after adjustments, as was experience of “no problems” in mobility (HR: 0.61; 95% CI: 0.43–0.87; p = 0.006). “No problems” in mobility was negatively correlated with musculoskeletal damage accrual (φ = −0.08; p = 0.008) and associated with a lower hazard to accrue musculoskeletal damage in Cox regression analysis (HR: 0.38; 95% CI: 0.19–0.76; p = 0.006).

Conclusion

Experience of EQ-5D-3L FHS and “no problems” in mobility after therapeutic intervention heralded reduced hazard to accrue subsequent organ damage, especially musculoskeletal damage, suggesting that optimisation of these health-related quality of life aspects constitutes a clinically relevant treatment target in patients with SLE, along with clinical and laboratory parameters.
The calibration of esophageal pressure by proper esophageal balloon filling volume: A clinical study
Authors: Jiang Jing , Su Longxiang , Cheng Wei , Wang Chunfu , Rui Xi , Tang Bo , Zhang Hongmin , He Huaiwu , Long Yun
Published: December 2022

Background

Esophageal pressure (Pes) can be used as a reliable surrogate for pleural pressure, especially in critically ill patients requiring personalized mechanical ventilation strategies. How to choose the proper esophageal balloon filling volume and then find the optimal value of esophageal pressure remains a challenge. The study aimed to assess the feasibility of catheters for Pes monitoring in mechanically ventilated patients.

Materials and methods

Twelve patients under pressure-controlled mechanical ventilation were included in this study. Raw esophageal pressure was recorded at different balloon filling volumes. Then, the P-V curves were determined. VWORK was the intermediate linear section on the end-expiratory P-V curve, and VBEST was the filling volume providing the maximum difference between Pes at end-inspiration and end-expiration. The raw value of Pes was recorded, and the calibrated values of Pes were calculated by calculating the esophageal wall pressure (Pew) and esophageal elastance (Ees).

Results

Twenty-four series of Pes measurements were performed. The mean VMIN and VMAX were 2.17 ± 0.49 ml (range, 1.0–3.0 ml) and 6.79 ± 0.83 ml (range, 5.0–9.0 ml), respectively, whereas VBEST was 4.69 ± 0.16 ml (range, 2.0–8.0 ml). Ees was 1.35 ± 0.51 cm H2O/ml (range, 0.26–2.38 cm H2O/ml). The estimated Pew at VBEST was 3.16 ± 2.19 cm H2O (range, 0–7.97 cm H2O). Patients with a body mass index (BMI) ≥ 25 kg/m2 had a significantly lower VMAX (5.88 [5.25–6] vs. 7.25 [7–8] ml, p = 0.006) and a significantly lower VBEST (3.69 [2.5–4.38] vs. 5.19 [4–6] ml, p = 0.036) than patients with a BMI < 25 kg/m2. Patients with positive end-expiratory pressure (PEEP) ≥ 10 cm H2O had a lower VMIN and VBEST than patients with PEEP < 10 cm H2O, P > 0.05. Patients in the supine position had a higher esophageal pressure than those in the prone position with the same balloon filling volume.

Conclusions

Calibration of esophageal pressure to identify the best filling volume of esophageal balloon catheters is feasible. The esophageal pressure can be influenced by BMI, PEEP, and position. It is necessary to titrate the optimal inflation volume again when the PEEP values or the positions change.
Isolation, antibacterial screening, and identification of bioactive cave dwelling bacteria in Fiji
Authors: Pipite Atanas , Lockhart Peter J. , McLenachan Patricia A. , Christi Ketan , Kumar Dinesh , Prasad Surendra , Subramani Ramesh
Published: December 2022

Bacteria are well known producers of bioactive secondary metabolites, including some of the most effective antibiotics in use today. While the caves of Oceania are still largely under-explored, they form oligotrophic and extreme environments that are a promising source for identifying novel species of bacteria with biologically active compounds. By using selective media that mimicked a cave environment, and pretreatments that suppressed the growth of fast-growing bacteria, we have cultured genetically diverse bacteria from a limestone cave in Fiji. Partial 16S rRNA gene sequences from isolates were determined and compared with 16S rRNA gene sequences in EzBioCloud and SILVA data bases. Fifty-five isolates purified from culture had Actinomycete-like morphologies and these were investigated for antibacterial activity. Initial screening using a cross streak test with pathogenic bacteria indicated that 34 of the isolates had antibacterial properties. The best matches for the isolates are bacteria with potential uses in the manufacture of antibiotics and pesticides, in bioremediation of toxic waste, in biomining, in producing bioplastics, and in plant growth promotion. Nineteen bacteria were confirmed as Actinomycetes. Thirteen were from the genus Streptomyces and six from genera considered to be rare Actinomycetes from Pseudonocardia, Kocuria, Micromonospora, Nonomuraea. Ten isolates were Firmicutes from the genera Bacillus, Lysinbacillus, Psychrobacillus and Fontibacillus. Two were Proteobacteria from the genera Mesorhizobium and Cupriavidus. Our findings identify a potentially rich source of microbes for applications in biotechnologies.
Perilla frutescens as potential antimicrobial modifier to against forage oat silage spoilage
Authors: Li Xiaomei , Chen Fei , Xiong Yi , Guo Linna , Xu Jingjing , Lin Yanli , Ni Kuikui , Yang Fuyu
Published: December 2022

The aim of this study was to investigate the influence of Perilla frutescens, alone or in combination with Lactobacillus plantarum a214 or citric acid, on forage oat silage quality, bacterial and fungal microbiological profile during ensiling and aerobic exposure. With the exception of Perilla frutescens, all additives could improve silage quality of forage oat based on lower ammonia-nitrogen content and higher residual of water soluble carbohydrates during anaerobic fermentation compared to control silage, especially in Perilla frutescens combined with citric acid (CAPF). Lactobacillus was the dominant bacteria in all silages, while CAPF group increased the relative abundance of Lactobacillus lindneri and Lactobacillus brevis compared to control silage. The application of Perilla frutescens suppressed the relative abundance of yeasts such as Pichia fermentans and Wickerhamomyces anomalus in response to aerobic exposure, especially in CAPF treatment, leading to high acetic acids and lower dry matter loss, as well as good aerobic stability. Therefore, Perilla frutescens, alone or in combination with citric acid, has potential to improve aerobic stability of forage oat silage by shifting bacterial and fungal community composition, and can be used as new additive to prepare high-quality silage for animal production.
Advances and perspectives on perylenequinone biosynthesis
Authors: Deng Huaxiang , Liang Xinxin , Liu Jinbin , Zheng Xiaohui , Fan Tai-Ping , Cai Yujie
Published: December 2022

Under illumination, the fungal secondary metabolites, perylenequinones (PQs) react with molecular oxygen to generate reactive oxygen species (ROS), which, in excess can damage cellular macromolecules and trigger apoptosis. Based on this property, PQs have been widely used as photosensitizers and applied in pharmaceuticals, which has stimulated research into the discovery of new PQs and the elucidation of their biosynthetic pathways. The PQs-associated literature covering from April 1967 to September 2022 is reviewed in three sections: (1) the sources, structural diversity, and biological activities of microbial PQs; (2) elucidation of PQ biosynthetic pathways, associated genes, and mechanisms of regulation; and (3) advances in pathway engineering and future potential strategies to modify cellular metabolism and improve PQ production.
Endoscopic surgery for spontaneous supratentorial intracerebral haemorrhage: A systematic review and meta-analysis
Authors: Hallenberger Tim J. , Guzman Raphael , Bonati Leo H. , Greuter Ladina , Soleman Jehuda
Published: December 2022

Introduction

Treatment for spontaneous supratentorial intracerebral haemorrhage (SSICH) is limited and consist of either best medical treatment (BMT) or surgical hematoma evacuation. Treatment methods and choice of surgical technique are debated, and so far, no clear advantage of endoscopic surgery (ES) over conventional craniotomy (CC) or BMT was shown. The aim of this systematic review and meta-analysis was to investigate the differences in outcome, morbidity, and mortality between ES and CC or BMT.

Methods

We systematically searched Embase and PubMed databases for randomised controlled trials comparing ES to CC or BMT. The primary outcome was favourable functional outcome after 6 months. Secondary outcomes were morbidity and mortality rates and duration of surgery.

Results

Seven articles were eligible for the outcome analysis with 312 subjects in the control (216 CC, 96 BMT) and 279 in the treatment group (ES). Compared to BMT, ES showed significantly improved favourable functional outcome (RR 1.93 [1.12; 3.33], p = 0.02) and mortality rates (RR 0.63 [0.44; 0.90], p = 0.01). No significant difference in favourable functional outcome and mortality was seen in ES compared to CC (RR 2.13 [0.01; 737], p = 0.35; RR 0.42 [0.17; 1.05], p = 0.06). ES showed significantly lower morbidity (RR 0.41 [0.29; 0.58], p < 0.01), and overall infection rates (RR 0.33 [0.20; 0.54], p < 0.01) compared to CC. Duration of surgery was significantly shorter for ES compared to CC (SMD −3.17 [−4.35; −2.00], p < 0.01).

Conclusion

ES showed significantly improved favourable functional outcome and mortality rates compared to BMT while showing reduced length of surgery and lower complication rates compared to CC. Therefore, ES appears a promising approach for treatment of SSICH justifying further prospective trials.

Systematic review registration

PROSPERO, identifier: CRD42020181018.
Hemispheric asymmetries for music and speech: Spectrotemporal modulations and top-down influences
Authors: Zatorre Robert J.
Published: December 2022

Hemispheric asymmetries in auditory cognition have been recognized for a long time, but their neural basis is still debated. Here I focus on specialization for processing of speech and music, the two most important auditory communication systems that humans possess. A great deal of evidence from lesion studies and functional imaging suggests that aspects of music linked to the processing of pitch patterns depend more on right than left auditory networks. A complementary specialization for temporal resolution has been suggested for left auditory networks. These diverse findings can be integrated within the context of the spectrotemporal modulation framework, which has been developed as a way to characterize efficient neuronal encoding of complex sounds. Recent studies show that degradation of spectral modulation impairs melody perception but not speech content, whereas degradation of temporal modulation has the opposite effect. Neural responses in the right and left auditory cortex in those studies are linked to processing of spectral and temporal modulations, respectively. These findings provide a unifying model to understand asymmetries in terms of sensitivity to acoustical features of communication sounds in humans. However, this explanation does not account for evidence that asymmetries can shift as a function of learning, attention, or other top-down factors. Therefore, it seems likely that asymmetries arise both from bottom-up specialization for acoustical modulations and top-down influences coming from hierarchically higher components of the system. Such interactions can be understood in terms of predictive coding mechanisms for perception.
Spine morphogenesis and synapse formation in tubular sclerosis complex models
Authors: Shimada Tadayuki , Yamagata Kanato
Published: December 2022

Tuberous sclerosis complex (TSC) is caused by mutations in the Tsc1 or Tsc2 genes, whose products form a complex and inactivate the small G-protein Rheb1. The activation of Rheb1 may cause refractory epilepsy, intellectual disability, and autism, which are the major neuropsychiatric manifestations of TSC. Abnormalities in dendritic spines and altered synaptic structure are hallmarks of epilepsy, intellectual disability, and autism. In addition, spine dysmorphology and aberrant synapse formation are observed in TSC animal models. Therefore, it is important to investigate the molecular mechanism underlying the regulation of spine morphology and synapse formation in neurons to identify therapeutic targets for TSC. In this review, we focus on the representative proteins regulated by Rheb1 activity, mTORC1 and syntenin, which are pivotal downstream factors of Rheb1 in the alteration of spine formation and synapse function in TSC neurons.