bluuebunny/crossref_metadata_2025 huggingface dataset:
Postoperative Effects of Continuous Dexmedetomidine Infusion During Gynecological Laparoscopy: A Randomized, Placebo-Controlled, Double-Blind, Single Center Clinical Trial
Authors: Liu SiYuan , Wang Binbin , Gao Yongtao , Xu Xingguo
Published: April 2020

Background: Dexmedetomidine(DEX) has proven to be an effective adjuvant to anesthetics owing to its sympatholytic, analgesic, and sedative properties. This study was designed to investigate the postoperative effects of continuous DEX infusion during gynecological laparoscopy and to evaluate the safety of this treatment. Methods: Sixty patients undergoing selective gynecological laparoscopy with general anesthesia were randomly assigned into DEX group (group D )and normal saline group (group N). DEX was infused at 0.5ug/kg/h during anesthesia maintenance in group D while normal saline was infused at the same rate in group N. The primary outcome measures were heart rate and blood pressure at extubation, 1, 2, 3, 4, 5, 6, 7, 8, 12, 24 and 48 hours after operation, NRS scores at 1, 2, 3 ,4, ,8,12,24 hours after surgery and OAA/S scores after extubation. The secondary outcome measures were time to extubation, propofol and remifentanil dosage during anesthesia, postoperative adverse events, and postoperative hospital stay.Results: Systolic and diastolic blood pressures were lower in group D at extubation when compared with group N (P<0.05), but not different at the first postoperative hour or after. HR was lower both at extubation and the first postoperative hour in group D (P<0.05). NRS scores were lower in patients given DEX both at rest and movement at the first postoperative hour (P<0.05). Time to extubation and OAA/S scores after extubation were not different between groups. Postoperative respiratory depression, shivering and pruritus didn’t occur in this research. There were no significant differences in the incidence rate of postoperative adverse events or postoperative hospital stay between the groups.Conclusions: Continuous infusion of DEX during gynecological laparoscopy provided better hemodynamic stability at extubation and the effects on HR continued until 1 hour after operation. DEX administration was associated with lower NRS scores at the first postoperative hour without excessive sedation. DEX is a useful and safe adjuvant for general anesthesia during gynecological laparoscopy.
Clinicopathological significance of Sox10 expression in triple-negative breast carcinoma
Authors: Jin Linfang , Qin Chenglin , Qi Xiaowei , Hong Tingting , Yang Xiaodong , Zhu Xun
Published: April 2020

Purpose The present study aimed to investigate the Sox10 expression in the pathological diagnosis of triple-negative breast cancer (TNBC). Furthermore, its correlation with the clinicopathological characteristics and disease-free survival rate in patients with TNBC was also evaluated to identify the diagnostic utility of Sox10 as a reliable biomarker for diagnosis and prognosis of TNBC. Methods Using immunohistochemistry, we identified the expression of Sox10, GATA-3, FOXA1, GCDFP15 and MGB in 376 cases of primary invasive breast cancer, and 77 cases of metastatic breast cancer. The expression of Sox10 in different molecular subtypes of primary invasive breast cancer and metastatic breast cancer were also compared. Furthermore, the correlation between Sox10 expression and clinicopathological parameters and disease-free survival (DFS) of patients with primary TNBC were also analyzed. Results Expression of Sox10 was only detected in the myoepithelial cells of normal breast, but not in any other types of cells, including luminal cell and fibroblasts. The positive rate of Sox10 in primary and metastatic TNBC was significantly higher than that in the other two types (P < 0.001, P < 0.001, respectively). The sensitivity and specificity of Sox10 expression in primary TNBC and metastatic TNBC were significantly lower than GATA-3, significantly higher than FOXA1, GCDFP15, and MGB (P < 0.001, P = 0.0004, P = 0.0064, P = 0.0229, respectively). In 71 cases of primary TNBC, a higher expression rate of Sox10 was significantly associated with high-grade tumors, late-stage tumors, and tumors with involvement of four or more lymph node metastases (P = 0.0145, P = 0.0105, P = 0.0249, respectively). Conclusion Sox10 may be used as a novel reliable putative marker for the diagnosis of TNBC. Notably, Sox10 combined with GATA-3 expression may serve as a supplementary differential diagnostic biomarker for primary and metastatic TNBC. Besides, Sox10 may be a good predictor of the prognosis of primary and metastatic TNBC. This study also highlights the significance of targeting Sox10 as a promising potential therapeutic target gene for TNBC therapy.
Integrative characterization and validation of tumor-associated immune signature combined with immune-infiltration analysis in clear cell renal cell carcinoma
Authors: Chen Hongxi , Xie Jinliang , Jin Peng
Published: April 2020

Background We aimed to explore and validate a prognostic immune signature for predicting the prognosis of ccRCC patients, combined with immune-infiltration analysis. Methods We obtained the multi-omics data from public datasets. Differential analysis was performed by edgeR package. Prognostic immune signature was identified by univariate Cox analysis, and we constructed an integrative tumor-associated immune Genes (TAIG) model from the multivariate Cox results. Functional analysis was conducted to uncover the related crosstalk. Importantly, we implemented the CIBERSORT algorithm to estimate the immune cell fractions in ccRCC samples and analyzed the differential abundance of tumor-infiltrating immune cells in two TAIG groups using Wilcoxon rank-sum test. The prognostic role of differential immune cells was further assessed by Kaplan-Meier analysis. In addition, we investigated the associations of single immune signature with specific immune cells. Results A total of 628 ccRCC patients were included in our integrative analysis, including 537 ccRCC patients in discovery group and 91 patients in validation group. Then, we identified the 14 key immune signatures. The AUC was 0.802 and patients with higher TAIG suffered from worse prognosis. Correlation analysis indicated that TAIG correlated tightly with clinical variables and TMB. Moreover, functional analysis also implicated the immune-related GO items or crosstalk. Therefore, we discovered the relationships of TAIG with tumor-infiltrating immune cells. The differential abundance of immune cells showed significant prognostic difference consisted of memory activated CD4 + T cell, T follicular helper cells, T regulatory cell, and so on. Moreover, we also characterized the associations between identified signature with specific immune cells. Finally, the 5-year AUC in ICGC cohort was 0.72, suggesting the robustness of TAIG that we constructed. Conclusions Totally, our team characterized the tumor-associated immune signature in ccRCC and further uncovered the prognostic tumor-infiltrating immune cells related with TAIG, providing a comprehensive foundation for investigating mechanisms or individualized immunotherapy.
Sciellin stimulates gallbladder cancer proliferation and tumor-association thrombosis via formation of neutrophil extracellular traps
Authors: Li Yang , Ren Tai , Yang Bo , Miao Huijie , Liu Liguo , Li Yongsheng , Yuan Ruiyan , Song Xiaoling , Cai Chen , Yang Yang , Hu Yunping , Chen Chen , Jiang Chengkai , Zhang Yijian , Liu Ying-bin
Published: April 2020

Background：Apart from primary tumor development and metastasis, cancer-associated thrombosis is the second cause of cancer death in solid tumor malignancy. However, the mechanistic insight into the development of gallblader cancer (GBC) and cancer-associated thrombosis remains unclear. This study aimed to investigate the mechanistic role of Sciellin （SCEL） in GBC cell proliferation and the development of venous thromboembolism. Methods: The expression level of SCEL was determined by immunohistochemical staining. Roles of SCEL in gallbladder cancer cell were determined by molecular and cell biology methodsResults: SCEL was markedly upregulated in GBC and associated with advanced TNM stages and a poor prognosis. Furthermore, SCEL interacted with EGFR and stabilized EGFR expression that activates downstream PI3K and Akt pathway, leading to cell proliferation. In addition, SCEL induces tumor cell IL-8 production that stimulates the formation of neutrophil extracellular traps (NETs), accelerating thromboembolism. In xenografts, SCEL-expressing GBCs developed larger tumors and thrombosis compared with control cells. Conclusions: The present results indicate that SCEL promotes GBC cell proliferation and induces NET-associated thrombosis , thus serving as a potential therapeutic target.
MiRNA-485-5p inhibited the proliferation and promoted the apoptosis of hepatocellular carcinoma cells by targeting MUC1
Authors: Li Ming , Zhu Dong , Peng Xiaozhen , Liu Pinyue , Yang Fen , Luo Hai , Zhang Xuemei , Liu Fang , Wei Le
Published: April 2020

Background The downregulation of miRNA-485-5-p was related to prognosis of various cancers, including hepatocellular carcinoma (HCC), while MUC1 was aberrantly expressed in many cancers and could be a biomarker for prognosis and diagnosis of cancers. The aim of the study was to investigate whether the miRNA-485-5p inhibited proliferation and promoted apoptosis by targeting MUC1 in HCC cells.Methods The expression of miRNA-485-5p in patients with HCC was measured by qPCR, and the epxression of MUC1 was detected by qPCR, western blot and immunohistochemistry. Bioinformatics and dual luciferase assay verified the targeting relationship between miRNA-485-5p and MUC1 in HCC cells. Cell proliferation was detected by CCK-8 assay. Cell apoptosis was detected by flow cytometry.Results The expression of miRNA-485-5p in HCC tissues is downregulated than that in adjacent tissue. However, the exprssion of MUC1 is opposite to the expression of miRNA-485-5p in the patients with HCC. Silencing MUC1 and overexpression miRNA-485-5p both inhibit proliferation and promote apoptosis in HCC cells. MiRNA-485-5p directly targete to the binding site of the MUC1 3’UTR and downregulated the expression of MUC1. Overexpression of MUC1 and miRNA-485-5p reverse the effect of miRNA-485-5p on the cell proliferation and apoptosis.Conclusion MiRNA-485-5p inhibits the proliferation and facilitates the apoptosis by negatively regulating the expression of MUC1 in HCC cells.
Electrical properties and aging behavior of nickel manganite thin films co-doped with Cu and Zn annealed at low temperature
Authors: Le Duc Thang , Ju Heongkyu
Published: April 2020

Nickel-manganite-based thin films with a negative temperature coefficient of resistance (NTCR) characteristic were prepared from (Ni 0.2 Mn 2.8– x Cu x )Cl 2 (0.010£ x £0.040) solutions using the liquid flow deposition (LFD) method. The influence of Cu on the electrical properties of the films annealed at 400 °C was investigated. Adding Cu ( x = 0.025) was found to quite effectively lower the electrical resistivity ( ρ ) of the films to 200 W cm, whereas their sensitivity behavior was significantly degraded. Interestingly, the absolute temperature coefficient of resistance ( TCR ) of the films was enhanced by further adding 0.01 mol Zn along with Cu, without a remarkable increase in ρ . TCR = 2.98% K – 1 and ρ = 880 W cm at room temperature were attained for the Zn-containing specimen with 0.020 mol Cu doping. Moreover, the films with (Zn,Cu) co-doped compositions showed a much higher electrical stability than the Zn-free films.
Computerized tomography scan findings of a patient with severe enterocolitis associated with the coronavirus disease 2019: a case report
Authors: Takifuji Katsunari , Terasawa Hiroshi , Oka Masami , Sahara Masaki , Hara Tomoko , Itoh Hidekazu
Published: April 2020

Background: Currently, there is no available study about the occurrence of enterocolitis associated with the coronavirus disease 2019 (COVID-19) among adults. Case presentation: A 56-year-old man had lower abdominal pain, distension, diarrhea, and bloody stool. Moreover, the patient presented with fever and general fatigue. Laboratory examination did not reveal leukocytosis nor neutrophilia. However, the patient’s C-reactive protein level increased to 4.9 (normal range: 0.0–0.3) mg/dL. Both sputum and stool cultures had normal results. However, abdominal computed tomography (CT) scan revealed wall thickness mainly in the submucosal layer of the left part of the large intestine. Thus, real-time polymerase chain reaction test of throat swabs was performed for the diagnosis of COVID-19, and positive results were obtained. Conclusions: We report the first case of enterocolitis correlated to COVID-19, and the patient had positive abdominal CT scan findings. This imaging modality could be effective for the diagnosis of enterocolitis associated with COVID-19.
COMMODITY STUDY OF DEVELOPED CUPCAKES OF ORGANIC RAW MATERIALS
Authors: Tkachenko Alina , Syrokhman Ivan , Basova Yulia , Kobischan Anna , Artemenko Anna , Kovalchuk Khrystyna , Kalashnyk Olena , Katruk Mariia , Zakharchyn Roman , Havrylyshyn Volodymyr
Published: April 2020

For widening the assortment of floury confectionary products of organic raw materials, recipes of new cupcakes of sandy dough “Cosmic” and “Lunik” were offered.

The recipe of cakes “Cosmic” included the organic raw materials: rice flour, grinded ginger, lemongrass powder, coconut sugar, creamy butter and sea buckthorn oil, mélange of chicken eggs, black ashberry jam. The recipe of cakes “Lunik” included rice flour, combined with spelt one, lemongrass powder, coconut sugar, creamy butter and sea buckthorn oil, mélange of chicken eggs, organic blackberry jam. A control sample was baskets with jelly and fruits, prepared by the traditional recipe. The main difference of the offered samples is in fact that they are prepared of the organic raw materials only. Margarine is a fatty base of the control sample. It is offered to replace the fatty base by creamy butter and sea buckthorn oil. The production technology of a sandy semi-product consists of four stages: preparation of raw materials for production, preparation of a sandy semi-product, its formation, baking. This paper offers the developed scale of organoleptic estimation of the cupcake quality and presents results of studying its food value. The highest quality level by organoleptic parameters was fixed in the “Lunik” sample – 0.96; in the “Cosmic” sample, the quality level is also rather high – 0.95; the control sample differed by much lower quality level – 0.83. The developed samples differed by lower energetic value, namely, 351.00 kcal and 313.50 kcal in the “Cosmik” and “Lunik” samples, respectively, whereas for the control sample this number was 433.70 kcal. The offered recipes of cupcakes included the higher protein content than the control – 7.40 and 7.58g/100 g for “Cosmic” and “Lunik” samples, respectively, whereas the protein content in the control was 5.40 g/100 g.
INVESTIGATION OF THE INTENSIVE TECHNOLOGY OF FOOD SPROUTS USING ORGANIC ACIDS
Authors: Kovaliova Olena , Chursinov Yuriy , Kalyna Viktoriia , Khromenko Tatyana , Kunitsia Ekaterina
Published: April 2020

The features of the intensive technology of getting food sprouts with organic acids as an intensifier of germination were studied. The aim was to establish the features of the intensive technology of producing sprouts of different crops with using organic acids at germination (butanedioic, 3-pyridine carbonic, pteroylglutamic). It is important to search new and safe germination stimulators of universal use. Such substances are just the studied organic acids, because positive changes of quality parameters of a ready product are traced at their use in the sprout technology. There was studied the sprout technologies that includes washing, disinfection, step-by-step air-water soaking of grains from different crops and their germination. As a disinfectant and growth stimulator at the stage of grain material soaking, there were used water solutions of the organic acids in the concentration diapason from 0.025 to 2.5 g/l. Due to their use, it became possible to get high-quality healthy food products, namely sprouts of different crops. Organic acid solutions stimulate the germination process and allow to get an essentially higher amount of high-quality sprouts in shorter terms without chemical toxic admixtures. The research results of the influence of the mentioned organic acids on germination indices of different grains materials are presented. Optimal values of concentrations of active substances in solutions have been established. Comparing with the classic technology of using these acids as a growth stimulator for sprouts allows to decrease the total duration of material germination in 1.5–2 times. Due to that it becomes possible to initiate the industrial production of sprouts. The experimental studies proved the effectiveness of using organic acids at getting living sprouts. It is demonstrated, that their use allows not only to intensify grains germination, but also favors more active formation of sprouts. The presented technology of producing sprouts of different crops is innovative. The obtained grain raw materials may be used independently or as an important component of new food products.
Detection and Identification of Microbial Contaminant in Bakery Products in Yogyakarta City, Indonesia
Authors: Ferdian Evelyn , Ariestanti Catarina Aprilia , Budiarso Tri Yahya
Published: April 2020

Bread has been chosen as an alternative food because of its availability. In Indonesia, consumption of breads is increased due to the change of diet pattern into packed and ready-made meals. Therefore, it is important to raise the awareness of bakery products quality. The purpose of this study was to detect and identify the microbial contaminant in bakery products in Yogyakarta City, Indonesia. Bacterial colonies from expired bakery products were isolated into pure isolate then confirmed by API Staph and Polymerase Chain Reaction (PCR) method. The results showed there were five kinds of gram-positive bacteria. Isolated bacteria identified by API were Bacillus cereus (52.8%), Bacillus subtilis (97.7%), Staphylococcus aureus (97.7%), Staphylococcus epidermidis (97,9%) and Staphylococcus saprophyticus (72,2% and 61,8%).
La transformación de Baja California en estado, 1931-1952
Authors: Taylor Hansen Lawrence Douglas
Published: April 2020

Este artículo analiza los factores más significativos respecto al proceso por medio del cual la región norte de la península de Baja California se transformó en estado durante el periodo comprendido entre 1931 —año en el cual se establecieron los Territorios Norte y Sur de Baja California— y 1952 —cuando se consumó esta transformación—. También se examinan los antecedentes del movimiento pro estado en la región a lo largo de la segunda mitad del siglo XIX y las primeras décadas del siglo XX. Se concluye que, aun cuando las iniciativas por parte del gobierno federal eran importantes para la realización de este fin, las de la administración regional también tuvieron un papel notable en este sentido, así como la propia voluntad por parte de los habitantes de la región para autogobernarse.
Emigración, inmigración y retorno: el ciclo de los inmigrantes mexicanos en Caléxico, California, EUA
Authors: Fimbres Durazo Norma
Published: April 2020

En este artículo se describe a una comunidad de inmigrantes mexicanos residentes legales en la ciudad de Caléxico, California. En primer término, se reseña brevemente la historia de la migración de México a Estados Unidos, en particular al condado de Imperial. En segundo, se describen las características del grupo de inmigrantes a partir de los datos provenientes de una encuesta aplicada a 407 inmigrantes mexicanos. Para finalizar, se presentan los resultados que proporciona la encuesta sobre las causas que intervinieron en la emigración, los motivos para inmigrar y la relación que existe entre el inmigrante y su país de origen, a través de la migración de retorno.
México y la apachería
Authors: Durazo Herrmann Francisco Julián
Published: April 2020

El propósito de este artículo es analizar, desde la perspectiva de la polítca exterior, las relaciones del gobierno mexicano con os apaches, una de las tribus nómadas que poblaban el septentrión mexicano. La tesis que sustenta es que, a pesar de que la política de México hacia los apaches no fue internacional ni en su objeto (los apaches nunca fueron tratados como una nación independiente) ni en sus instrumentos (la política apache fue diseñada y ejecutada principalmente por autoridades locales y estatales), esta política sí tenía un objetivo claro, aunque indirecto, de política exterior: consolidar el control mexicano sobre su frontera con los Estados Unidos.
Nostalgia por la tierra, nostalgia por el dolar: Guatemalan transnational lives and ideology of return migration
Authors: Moran-Taylor Michelle J.
Published: April 2020

This paper addresses the ideology of return among contemporary Guatemalan migrants living and working in the southwestern desert city of Phoenix in Arizona. In Phoenix’s metropolitan area, Guatemalans (both Ladino and Maya ethnic groups) add to the city’s cultural mosaic, and at the same time, are vital agents for change in Guatemalan society. Little is known about the processes and patterns behind Guatemalan migration despite the fact that over 10 percent of Guatemala’s population currently resides in the United States. Most Guatemalans in Phoenix, regardless of their length of residence in the United States, express longings for return to their homeland. In part, this strong notion of return prevails because the Guatemalan community in the Phoenix metropolitan area is not cohesive.
Antagonismos en las curvas de phillips con pendiente positiva: el caso de México y España
Authors: Roa Dueñas Rubén E.
Published: April 2020

En este documento se presentan los aspectos teóricos esenciales de la curva de Phillips, así como su evolución. Se hace un breve relato sobre las causas y efectos de la crisis mexicana de 1994, así como el consecuente y evidente incumplimiento de la aportación de Phillips. De igual forma se presenta la inclusión de España a la Comunidad Económica Europea a través de la consumación del tratado de Maastricht, su entorno y algunas de sus consecuencias, que en general muestra un carácter vigoroso y de prosperidad en su economía. Se concluye que la supuesta relación inversa entre desempleo e inflación que grosso modo nos muestra la curva de Phillips y los casos que no cumplen con esa norma, dan pie a debatir sobre el escenario y los medios que deben de emplear varias economías para aspirar a vencer a esta teoría y tender a orientar la curva hacia el origen, como en el caso español. Cabe anotar que lo importante no radica solo en vencer la teoría, sino en generar las condiciones necesarias para la consolidación de la economía, así como para su posterior plataforma de desarrollo económico y social sostenido.
Crecimiento, instituciones y convergencia en México considerando a la frontera norte
Authors: Celaya Tentori Diana , Díaz Bautista Alejandro
Published: April 2020

El presente estudio se centra en uno de los problemas económicos regionales fundamentales de nuestra época: el desenvolvimiento económico de la convergencia y el crecimiento en las regiones considerando a las instituciones. El estudio se ocupa de revisar la literatura teórica del crecimiento económico, y en particular por algunos de los desarrollos teóricos y empíricos que han tenido lugar recientemente en el campo de la convergencia económica. De igual forma, el estudio analiza la convergencia regional en México y aborda los fundamentos teóricos y empíricos en el cual las instituciones juegan un papel importante en el proceso de integración regional en México y en especial en la frontera norte. Finalmente, se realiza un análisis empírico de la convergencia regional de largo plazo para los estados de México, introduciendo una variable de corrupción dentro de las instituciones, una variable de frontera, controlando con una variable de capital humano regional. Los resultados estiman una tasa anual de convergencia de entre -0.018% a 4.1% para el periodo de 1970 a 2001, cuando se toman en cuenta los efectos de las variables institucionales, de capital humano y de frontera.
Migração feminina brasileira e a experiência do envelhecimento em Portugal: sexismo e outros “ismos”
Authors: Craveiro Queiroz Camila , Cabecinhas Rosa , Cerqueira Carla
Published: April 2020

Este artigo busca compreender como migrantes brasileiras, maiores de 50 anos, residentes em Portugal, percebem os estereótipos que lhes são imputados a partir do olhar do endogrupo, neste caso, a sociedade de acolhida. Por meio de entrevistas de Histórias de Vida, as quais analisamos sob o viés interseccional e o paradigma descolonial, contextualizamos os temas de maior recorrência nas falas destas migrantes, a saber: os meta-estereótipos, o mercado laboral, o envelhecer e algumas das estratégias de agência utilizadas nas tentativas de integração.

Palavras-chave: migrantes; gênero; racismo; idadismo; interseccionalidade.
Gloria Anzaldúa, uma chicana entre-fronteiras
Authors: Saraiva Palmeira Lara Virgínia
Published: April 2020

Se as fronteiras nacionais constituem-se enquanto experiências sociais complexas e particulares, elas também nos auxiliam a pensar nos processos de construção/reconstrução/fragmentação das identidades contemporâneas. No caso do artigo em questão, a problemática principal reside na complexidade com a qual se constitui o próprio ser humano. A personagem principal com a qual o diálogo é estabelecido é Glória Anzaldúa, considerada neste espaço a personificação das tensões que integram essa experiência social que é o viver entre-fronteiras. Além de apresentar a autora com uma breve biografia, foram analisadas três de suas principais obras com o objetivo de analisar de que maneira ela constrói sua teoria das fronteiras e a relaciona com a questão da identidade.
MAPEAMENTO E ANÁLISE MORFOESTRUTURAL DO MACIÇO DE JOÃO DO VALE (RN-PB)
Authors: Pereira de Oliveira George , Bernardino Diogo , Teixeira Batista Clístenes
Published: April 2020

Na Província Borborema, diversos maciços capeados por arenitos cenozoicos alçados a cotas de cerca de 750 m de altitude são apontados como indicadores de atividade neotectônica. Dentre esses maciços, a Serra de João do Vale permanece como o mais desprovido de estudos. O estudo dos lineamentos estruturais na área é importante para a identificação de pistas sobre o atual quadro morfoestrutural da Serra de João de Vale herdado de movimentações tectônicas cenozoicas. Sabendo-se disso, objetivou-se neste trabalho apresentar um mapeamento morfoestrutural preliminar para o referido maciço, baseado nas relações em superfície entre os lineamentos estruturais, os litotipos predominantes e as amplitudes topográficas relativas locais. Para conseguir isso se fez uso de técnicas de sensoriamento remoto. Identificou-se a ocorrência de sete compartimentos morfoestruturais. Espera-se que este trabalho seja o passo inicial para futuros trabalhos na região mais aprofundados no quadro.
Denaturant Induced Equilibrium Unfolding and Conformational Transitional Studies of Germinated Fenugreek β-Amylase Revealed Molten Globule like State at Low pH
Authors: Agrawal Dinesh Chand , Yadav Anjali , Khan Mohd. Asim , Kundu Suman , Kayastha Arvind M.
Published: April 2020

Background:

β-Amylase (EC 3.2.1.2) is a maltogenic enzyme, which releases β-maltose
from the non-reducing end of the substrates. The enzyme plays important roles for the production
of vaccine, maltiol and maltose rich syrups. Apart from these applications the enzyme protects
cells from abiotic as well as oxidative damage. The enzyme is βwell characterized in βplants and
microbes and crystal structures of β-amylases βhave been βobtained from sweet potato, soybean
and Bacillus cereus.

Objective:

Find out correlation between structural and functional stability induced by change in
pH, temperature and chaotropes.

Methods:

Activity, intrinsic fluorescence, extrinsic fluorescence, near- and far- ultraviolet circular
dichroism spectroscopic measurements were performed.

Results:

Peaks about 208 nm and 222 nm obtained by near-ultraviolet circular dichroism correspond
to α-helix whereas peak at 215 nm shows presence of β-sheet. At pH 2.0, absence of tertiary
structures, exposed of hydrophobic regions and presence of substantial secondary structures, revealed
the existence of molten globule like state. Temperature induced denaturation studies
showed that the enzyme was stable up to 75ºC and the process was found to be irreversible in nature.
Chaotropes dependent equilibrium unfolding studies revealed that at low concentration of
chaotropes, ellipticity and intrinsic fluorescence βintensity were βdecreased βwhereas βenzymatic
activity remained unchanged, which revealed fenugreek β-amylase is multi-domains enzyme and
catalytic βdomain βis more βstable compare to non-catalytic domain. Moreover, the transition was
sigmoidal and non-coincidental.

Conclusion:

Results indicate the probable existence of intermediate states that might perform significant
role in physiological process and biotechnological applications.
Computational Overview of Mycobacterial Thymidine Monophosphate Kinase
Authors: Gul Sana , Khalil Ruqaiya , Zaheer Ul-Haq  , Mubarak Mohammad S.
Published: April 2020

:

Tuberculosis (TB) ranks among the diseases with the highest morbidity rate with significantly high
prevalence in developing countries. Globally, tuberculosis poses the most substantial burden of mortality. Further,
a partially treated tuberculosis patient is worse than untreated; they may lead to standing out as a critical obstacle
to global tuberculosis control. The emergence of multi-drug resistant (MDR) and extremely drug-resistant (XDR)
strains, and co-infection of HIV further worsen the situation. The present review article discusses validated targets
of the bacterial enzyme thymidine monophosphate kinase (TMPK). TMPKMTB enzyme belongs to the nucleoside
monophosphate kinases (NMPKs) family. It is involved in phosphorylation of TMP to TDP, and TDP is
phosphorylated to TTP. This review highlights structure elucidation of TMP enzymes and their inhibitors study
on TMP scaffold, and it also discusses different techniques; including molecular docking, virtual screening, 3DPharmacophore,
QSAR for finding anti-tubercular agents.
Non-pharmacological Strategies Against Systemic Inflammation: Molecular Basis and Clinical Evidence
Authors: Pinto Rita Del , Pietropaoli Davide , Monaco Annalisa , Desideri Giovambattista , Ferri Claudio , Grassi Davide
Published: April 2020

Systemic inflammation is a common denominator to a variety of cardiovascular (CV) and non-CV
diseases and relative risk factors, including hypertension and its control, metabolic diseases, rheumatic disorders,
and those affecting the gastrointestinal tract. Besides medications, a non-pharmacological approach encompassing
lifestyle changes and other complementary measures is mentioned in several updated guidelines on the management
of these conditions. We performed an updated narrative review on the mechanisms behind the systemic
impact of inflammation and the role of non-pharmacological, complementary measures centered on lowering
systemic phlogosis for preserving or restoring a good global health. The central role of genetics in shaping the
immune response is discussed in conjunction with that of the microbiome, highlighting the interdependence and
mutual influences between the human genome and microbial integrity, diversity, and functions. Several plausible
strategies to modulate inflammation and restore balanced crosstalk between the human genome and the microbiome
are then recapitulated, including dietary measures, active lifestyle, and other potential approaches to manipulate
the resident microbial community. To date, evidence from high-quality human studies is sparse to allow
the unconditioned inclusion of understudied, though plausible solutions against inflammation into public health
strategies for global wellness. This gap claims further focused, well-designed research targeted at unravelling the
mechanisms behind future personalized medicine.
FoodSimplex as a Mean to Improve Portuguese Restaurants’ Goods Manufacturing Practices - Audit and Microbial Assessment
Authors: Baltazar Ana L. , Ferreira Ana , Manyes Lara , Mañes Jordi
Published: April 2020

Background:

The consumption of meals at resturants has increased mass catering establishments.
Such food services may present several risk factors for foodborne diseases, which have a
substantial negative impact on public health. The smallest business with no technical or financial
support struggles to comply with food safety European Regulation. The aim of this four years study
was to assess Good Manufacture Practices (GMP) in Portuguese restaurants applying a designed
food safety methodology, FoodSimplex, through audit data to determine good manufacture practices,
and microbiological sampling of the meals before and after its implementation, by food groups according
to their risk.

Methods:

FoodSimplex is a combination of four stages methodology, based on Hazards Analysis and
Critical Control Point (HACCP), combined with technical-functional and food safety audits, with
pretested checklist, training and microbial analysis (Mesophylic bacteria, Listeria monocytogenes, E.
coli, Coagulase-positive staphylococci and Salmonella spp) of the meals served.

Results:

Results of the food safety audit for GMP parameters showed a general improvement with
80% compliance with the checklist subitems. The ready-to-eat food samples presented, regarding the
total mesophilic aerobes count, a statistical significant change to acceptable and satisfactory condition
(p-value= 0.029). 100% compliance, with acceptable and satisfactory results was observed for
Listeria monocytogenes (p-value= 0.180) and for E. coli, coagulase-positive Staphylococci and Salmonella
spp., all the food samples presented satisfactory results in the study (100%).

Conclusion:

At the end of the investigation period, a decrease in bacterial populations in food samples
was observed with an improvement of the GMP audit data which indicated that the FoodSimplex
methodology improved the food safety status of these establishments.
Identifying Attack Models for Securing Cluster-based Recommendation System
Authors: Ahmad Amreen , Ahmad Tanvir , Tripathi Ishita
Published: April 2020

The immense growth of information has led to the wide usage of recommender systems
for retrieving relevant information. One of the widely used methods for recommendation is collaborative
filtering. However, such methods suffer from two problems, scalability and sparsity. In the
proposed research, the two issues of collaborative filtering are addressed and a cluster-based recommender
system is proposed. For the identification of potential clusters from the underlying network,
the Shapley value concept is used, which divides users into different clusters. After that, the
recommendation algorithm is performed in every respective cluster. The proposed system recommends
an item to a specific user based on the ratings of the item’s different attributes. Thus, it reduces
the running time of the overall algorithm, since it avoids the overhead of computation involved
when the algorithm is executed over the entire dataset. Besides, the security of the recommender
system is one of the major concerns nowadays. Attackers can come in the form of ordinary
users and introduce bias in the system to force the system function that is advantageous for them.
In this paper, we identify different attack models that could hamper the security of the proposed
cluster-based recommender system. The efficiency of the proposed research is validated by conducting
experiments on student dataset.
Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats
Authors: Sari Festi Artika , Sandhika Willy , Yuliawati Tri Hartini
Published: April 2020

Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value <0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.
Non-pharmacological Randomised Control Trial: Green Coconut (Cocos nucifera L.) Water to Reduce Dysmenorrhea Pain
Authors: Nugroho Fajar Ari , Putri Oktaviana Manda , Sariati Yuseva
Published: April 2020

Dysmenorrhea is a common cause of pain in young women. The discomfort of dysmenorrhea causes unstable emotions, sleep disturbances, diet changes, and increased stress and depression that can degrade women quality of life. This non-pharmacological Randomized Controlled Trial aimed to obtain the optimal dose of green coconut water as a magnesium source for reducing dysmenorrhea pain. Twenty-one participants divided into three groups. Treatment group 1 obtained 330 ml of green coconut water, treated group 2 obtained 165 ml of green coconut water, and the control group obtained 330 ml of mineral water where each dose repeated three times every 4 hours. VAS questionnaire used to measure the value of pain intensity before and after administration. The 330 ml green coconut water dose is the effective dose compared to control (VAS=4.14, p=0.000), while another dose has a weak effect (VAS=1.86, p=0.124). This finding proves that green coconut water can be a non-pharmacological alternative in handling dysmenorrhea pain.
Effect of Javanese Turmeric (Curcuma xanthorrhiza) Extract on Hepatitis Model of Alcohol-Induced Mice
Authors: Puteri Agnes Ilene Suprapto , Sandhika Willy , Hasanatuludhhiyah Nurina
Published: April 2020

Alcoholic hepatitis is a common cause of non-viral hepatitis. Javanese turmeric (Curcuma xanthorrhiza) contains xanthorrhizol and curcuminoid that have antioxidant and anti-inflammatory effects. The purpose of this research was to detect the effect of Curcuma xanthorrhiza in decreasing hydropic degeneration and lymphocytes in alcohol-induced mice. This experimental research used post-test only controlled design. Randomly, 35 mice were divided into five groups. Four groups were induced by alcohol 30% of 4ml/kgBW in 10 days, and one group was normal group (negative control). Three of the four alcohol-induced groups were given Curcuma xanthorrhiza extract at doses of 42, 84, and 168 mg/kgBW in 10 days, 1 hour after alcohol induction. Mice livers were taken for Hematoxylin Eosin (HE) staining.Â  Hydropic degeneration and lymphocytes in the liver lobules were examined under a microscope. The statistical analysis used in this research was the Kruskal-Wallis, the Mann-Whitney test, and the Independent Sample T Test. P-value is significant if <0.05. The result of this research were all doses of Javanese turmeric extract significantly reduced hydropic degeneration. Doses of 84 and 168 mg/kgBW also reduced inflammatory lymphocytes (p<0.05). The dose of 168 mg/kgBW reduced the lymphocyte inflammation maximally. There was no significant difference between increasing Javanese turmeric dose and decreasing hydropic degeneration and lymphocytes (p>0.05). This research shows that Javanese turmeric (Curcuma xanthorrhiza) can attenuate hepatitis by decreasing hydropic degeneration and lymphocytes in alcohol-induced liver.
ДИНАМІКА ЕЛЕКТРОМІОГРАФІЧНИХ ПОКАЗНИКІВ MUSCULUS MASSETER ТА MUSCULUS TEMPORALIS ПІД ВПЛИВОМ СТИМУЛЯЦІЇ ГРУПИ М’ЯЗІВ, ЯКІ ЗАБЕЗПЕЧУЮТЬ ТОНКУ КООРДИНАЦІЮ ЖУВАЛЬНОЇ МУСКУЛАТУРИ В ПРОЦЕСІ ОРТОПЕДИЧНОГО ЛІКУВАННЯ ПАЦІЄНТІВ З ПОВНОЮ ВІДСУТНІСТЮ ЗУБІВ
Authors: Сарапук В. І. , Рожко М. М.
Published: April 2020

Резюме. Мета: вивчити можливість застосування набору стандартних взірців H. Landt для тесту на здатність до тонкої координації жувальної мускулатури – МА-тест (Muscular-Ability) з ціллю підвищення адаптаційних можливостей до повних знімних пластинкових протезів (ПЗПП). Даний процес адаптації ми назвали МА-стимуляція.

Методи: електроміографія (показник сумарної амплітуди роботи musculus masseter та musculus temporalis (мкв)). Проведено дослідження у двох групах пацієнтів із повною відсутністю зубів. 1 група – 30 пацієнтів, яким ортопедичне лікування проводилося без попередньої МА-стимуляції. 2 група – 60 пацієнтів, яким перед ортопедичним лікуванням протягом 14 днів проводилася МА-стимуляція.

Результати. У 2 групі показник у стані відносного фізіологічного спокою знизився на 23,5 %, через 1 місяць після ортопедичного лікування ще на 14,1 %, через 3 місяці – ще на 9,2 %; при максимальному стисненні щелеп відбулося зростання показника на 29,8 %, через 1 і 3 місяці зростання досягнуло відповідно 36,4 % і 44,7 %; при максимальному стисненні щелеп з ватними валиками відбулося зростання показника на 26,8 %, через 1 і 3 місяці зростання досягнуло відповідно 27,1 % і 42,2 %.

У 1 групі показник у стані відносного фізіологічного спокою знизився через 1 місяць після ортопедичного лікування на 18,1 %, а через 3 місяці ще на 20,9 %; при максимальному стисненні щелеп зростання показника через 1 місяць не відбулося, а через 3 місяці зростання відбулося на 25,2 %; при максимальному стисненні щелеп з ватними валиками зростання відбулося тільки через 3 місяці після ортопедичного лікування на 25,1 %.

Висновки. У пацієнтів з повною відсутністю зубів МА-стимуляція сприяє швидкому активному підвищенню адаптаційних процесів до використання ПЗПП.
ОСОБЛИВОСТІ ВПЛИВУ МЕЛАТОНІНУ НА ХРОНОРИТМІЧНУ ОРГАНІЗАЦІЮ КИСЛОТОРЕГУЛЮВАЛЬНОЇ ФУНКЦІЇ НИРОК ПІД ВПЛИВОМ БЛОКАДИ СИНТЕЗУ МОНООКСИДУ НІТРОГЕНУ ЗА УМОВ ГІПОФУНКЦІЇ ШИШКОПОДІБНОЇ ЗАЛОЗИ
Authors: Семененко С. Б. , Тимофійчук І. Р. , Слободян К. В. , Семененко В. В. , Семененко Н. Ю.
Published: April 2020

Резюме. Про ритмічну організацію окремих функцій організму лікарям відомо давно. У здорових людей ритми фізіологічних процесів синхронізовані як між собою, так і з ритмами навколишнього середовища, що забезпечує оптимальнi умови функцiонування органiзму i є ознакою здоров’я.

Мета цього дослідження полягає у вивченні особливостей впливу мелатоніну на хроноритмічну організацію кислоторегулювальної функції нирок під впливом блокади синтезу монооксиду нітрогену за умов гіпофункції шишкоподібної залози.

Матеріали і методи. Досліди провели на 72 статевозрілих нелінійних самцях білих щурів масою 0,15-0,18 кг. Тварин утримували в умовах віварію при сталій температурі та вологості повітря на стандартному харчовому раціоні. Контрольну групу склали тварини (n=36), які перебували за умов звичайного світлового режиму (12.00С:12.00Т) упродовж семи діб. Досліджувану групу склали тварини (n=36), яким уводили N-нітро-L-аргінін (L-NNA) в дозі 20 мг/кг упродовж 7-ми діб за умов постійного освітлення (12.00С:12.00С) і паралельно мелатонін (МТ) в дозі 0,5 мг/кг упродовж 7-ми діб.

Обговорення результатів. З’ясування особливостей хроноритмічних перебудов кислоторегулювальної функції нирок за умов блокади синтезу монооксиду нітрогену (NO) і уведення МТ на тлі пригніченої активності шишкоподібної залози (ШЗ) є важливим для пізнання механізмів виникнення та розвитку патологічних станів, що удосконалить лікування ниркової патології.

Висновки. Таким чином, за умов L-NNA блокади синтезу монооксиду нітрогену і уведення мелатоніну в умовах гіпофункції шишкоподібної залози спостерігали хроноритмічні перебудови архітектоніки та фазової структури ритмів більшості показників кислоторегулювальної функції нирок.
СВІТЛООПТИЧНІ ТА СУБМІКРОСКОПІЧНІ ОСОБЛИВОСТІ ПАТОЛОГІЧНИХ ЗМІН У ГЕМОРОЇДАЛЬНИХ ВУЗЛАХ ВИДАЛЕНИХ РІЗНИМИ ІНСТРУМЕНТАМИ
Authors: Скрипко В. Д. , Попадинець О. Г. , Соломчак П. В.
Published: April 2020

Резюме. Геморойектомія– це операція, при якій задоволеність пацієнтів низька, хоча це часто виконувана операція в сучасній хірургічній практиці. Основною причиною невдоволення є виражений післяопераційний біль та інші ускладнення.Значна частина ускладнень пов’язана з видом хірургічного інструменту, яким видаляється гемороїдальний комплекс. Актуальним є пошук інструменту, який дозволяє ефективно розсікати тканини з мінімальними пошкоджуючими ефектами. З метою вивчення впливу металевого скальпеля, діатермокоагуляції та біполярної коагуляції на тканину гемороїдального комплексу у досліджуваних хворих проводили гістологічні дослідження.

При класичній закритій геморойектомії за Фергюсоному стінці кишки спостерігаються геморагічно-дистрофічні процеси, виражена дезорганізація сполучно-тканинних складових. При геморойектомії за Мілліганом-Морганом,виконаній моно полярною електрокоагуляційною установкою,у стінці кишки домінують набрякові зміни. При геморойектомії за Мілліганом-Морганом шляхом використання високочастотного електрозварювання м’яких тканин фіксується тонкий шар коагуляційного некрозу, збереженість оболонкової будови стінки кишки. Є незначний набряк міжклітинної основної речовини і явища стазу у дрібних судинах, мозаїчні острівці геморагічного просякання.

Застосування методу високочастотного електрозварювання м’яких тканин характеризується контрольованою, щадливою дією на тканини, зона латерального некрозу після біполярної коагуляції менша, ніж після застосування звичайної діатермії. Дисекція та коагуляція за допомогою біполярного високочастотного електрокоагулятора ЕК 300 М1характеризується суворо локальним ефектом, наявністю “біологічного зварювання” шарів, органу, який розсікали, й надійним гемостазом.
ТЕОРЕТИКО-ПРАВОВІ АСПЕКТИ РЕФОРМУВАННЯ СИСТЕМИ ОХОРОНИ ЗДОРОВ’Я
Authors: Чурпій І. К. , Мельник І. В. , Чурпій Н. В.
Published: April 2020

Резюме. В останні роки система охорони здоров'я в Україні зазнає постійних змін та модифікацій. Методом спроб та помилок уряд України намагається вибрати найбільш оптимальну систему надання медичних послуг для населення.

Україна одна з багатьох країн, що розвивається на просторах пострадянського простору, і більшість її галузей перебувають у стадії занепаду. Медичний сектор – не виняток.

У цій статті було проаналізовано основні теоретико-правові аспекти реформування системи охорони здоров'я, їхню рентабельність для населення України, їхню ефективність та ресурсні затрати.

Покращення поінформованості населення у юридичних аспектах новітніх реформ, які діють або ще готуються до вступу в дію, найближчим часом дозволить людям чіткіше розуміти, яких саме змін вони прагнуть, і коригувати роботу уряду.

Зміни кожної країни починаються з кожного жителя цієї країни. І коли кожен громадянин поважатиме себе, своє здоров'я та лікарську працю – саме тоді почнуться справжні зміни.

Сучасна медична установа має відповідати світовим трендам і забезпечувати якість надання медичних послуг шляхом впровадження вимог міжнародних стандартів, зокрема, системи менеджменту якості відповідно до міжнародного стандарту ISO 9001: 2008 з подальшою сертифікацією.