bluuebunny/crossref_metadata_2025 huggingface dataset:
Accurate Channel Estimation and Adaptive Underwater Acoustic Communications Based on Gaussian Likelihood and Constellation Aggregation
Authors: Wang Liang , Qiao Peiyue , Liang Junyan , Chen Tong , Wang Xinjie , Yang Guang
Published: March 2022

Achieving accurate channel estimation and adaptive communications with moving transceivers is challenging due to rapid changes in the underwater acoustic channels. We achieve an accurate channel estimation of fast time-varying underwater acoustic channels by using the superimposed training scheme with a powerful channel estimation algorithm and turbo equalization, where the training sequence and the symbol sequence are linearly superimposed. To realize this, we develop a ‘global’ channel estimation algorithm based on Gaussian likelihood, where the channel correlation between (among) the segments is fully exploited by using the product of the Gaussian probability-density functions of the segments, thereby realizing an ideal channel estimation of each segment. Moreover, the Gaussian-likelihood-based channel estimation is embedded in turbo equalization, where the information exchange between the equalizer and the decoder is carried out in an iterative manner to achieve an accurate channel estimation of each segment. In addition, an adaptive communication algorithm based on constellation aggregation is proposed to resist the severe fast time-varying multipath interference and environmental noise, where the encoding rate is automatically determined for reliable underwater acoustic communications according to the constellation aggregation degree of equalization results. Field experiments with moving transceivers (the communication distance was approximately 5.5 km) were carried out in the Yellow Sea in 2021, and the experimental results verify the effectiveness of the two proposed algorithms.
Hybrid Magnetic–Inductive Angular Sensor with 360° Range and Stray-Field Immunity
Authors: Brajon Bruno , Lugani Lorenzo , Close Gael
Published: March 2022

Magnetic and inductive sensors are the dominant technologies in angular position sensing for automotive applications. This paper introduces a new angular sensor: a hybrid concept combining the magnetic Hall and inductive principles. A magnetic Hall transducer provides an accurate angle from 0° to 180°, whereas an inductive transducer provides a coarse angle from 0° to 360°. For this novel concept, a hybrid target with a magnetic and inductive signature is also needed. Using the two principles at the same time enables superior performances, in terms of range, compactness and robustness, that are not possible when used separately. We realized and characterized a prototype. The prototype achieves a 360° range, has a high accuracy and is robust against mechanical misalignments, stray fields and stray metals. The measurement results demonstrate that the two sensing principles are completely independent, thereby opening the doors for hybrid optimum magnetic–inductive designs beyond the usual trade-offs (range vs. resolution, size vs. robustness to misalignment).
New Caries Diagnostic Tools in Intraoral Scanners: A Comparative In Vitro Study to Established Methods in Permanent and Primary Teeth
Authors: Schlenz Maximiliane Amelie , Schupp Berenike , Schmidt Alexander , Wöstmann Bernd , Baresel Ingo , Krämer Norbert , Schulz-Weidner Nelly
Published: March 2022

The aim of this in vitro study was to systematically investigate new caries diagnostic tools, including three intraoral scanners, and compare them to established diagnostic methods. For a standardized analysis of occlusal and proximal caries lesions, human permanent and primary teeth (n = 64) were embedded in models and investigated in a phantom head using six different caries diagnostic methods: visual examination, bitewing radiography, Diagnocam (KaVo, Biberach, Germany), Trios 4 (3Shape, Copenhagen, Denmark), iTero Element 5D (Align Technology, San José, CA, USA), and Planmeca Emerald S (Planmeca, Helsinki, Finland). The diagnostic methods were investigated and compared to reference µ-CT for permanent and primary teeth separately. For occlusal caries diagnostics in permanent teeth, the best agreement to the reference (reliability) was obtained for Planmeca Emerald S (ĸ = 0.700), whereas in primary teeth, for visual examination (ĸ = 0.927), followed by Trios 4 (ĸ = 0.579). Regarding proximal caries diagnostics, bitewing radiography, as the gold standard, exhibited the highest agreement for permanent (ĸ = 0.643) and primary teeth (ĸ = 0.871). Concerning the analysis of the diagnostic quality (sensitivity and specificity) using receiver operating characteristic (ROC) curve analysis, comparable findings were obtained for area under curve (AUC) values as for reliability. No diagnostic method could be identified that is generally suitable for occlusal and proximal lesions in both dentitions. Overall, caries diagnostics with intraoral scanners seem to be interesting tools that should be further investigated in clinical studies.
Shedding Light on Capillary-Based Backscattering Interferometry
Authors: Mulkerns Niall M. C. , Hoffmann William H. , Lindsay Ian D. , Gersen Henkjan
Published: March 2022

Capillary-based backscattering interferometry has been used extensively as a tool to measure molecular binding via interferometric refractive index sensing. Previous studies have analysed the fringe patterns created in the backscatter direction. However, polarisation effects, spatial chirps in the fringe pattern and the practical impact of various approximations, and assumptions in existing models are yet to be fully explored. Here, two independent ray tracing approaches are applied, analysed, contrasted, compared to experimental data, and improved upon by introducing explicit polarisation dependence. In doing so, the significance of the inner diameter, outer diameter, and material of the capillary to the resulting fringe pattern and subsequent analysis are elucidated for the first time. The inner diameter is shown to dictate the fringe pattern seen, and therefore, the effectiveness of any dechirping algorithm, demonstrating that current dechirping methods are only valid for a subset of capillary dimensions. Potential improvements are suggested in order to guide further research, increase sensitivity, and promote wider applicability.
Laboratory Hyperspectral Image Acquisition System Setup and Validation
Authors: Morales Alejandro , Horstrand Pablo , Guerra Raúl , Leon Raquel , Ortega Samuel , Díaz María , Melián José M. , López Sebastián , López José F. , Callico Gustavo M. , Martel Ernestina , Sarmiento Roberto
Published: March 2022

Hyperspectral Imaging (HSI) techniques have demonstrated potential to provide useful information in a broad set of applications in different domains, from precision agriculture to environmental science. A first step in the preparation of the algorithms to be employed outdoors starts at a laboratory level, capturing a high amount of samples to be analysed and processed in order to extract the necessary information about the spectral characteristics of the studied samples in the most precise way. In this article, a custom-made scanning system for hyperspectral image acquisition is described. Commercially available components have been carefully selected in order to be integrated into a flexible infrastructure able to obtain data from any Generic Interface for Cameras (GenICam) compliant devices using the gigabyte Ethernet interface. The entire setup has been tested using the Specim FX hyperspectral series (FX10 and FX17) and a Graphical User Interface (GUI) has been developed in order to control the individual components and visualise data. Morphological analysis, spectral response and optical aberration of these pushbroom-type hyperspectral cameras have been evaluated prior to the validation of the whole system with different plastic samples for which spectral signatures are extracted and compared with well-known spectral libraries.
Systematic Mapping Literature Review of Mobile Robotics Competitions
Authors: Brancalião Laiany , Gonçalves José , Conde Miguel Á. , Costa Paulo
Published: March 2022

This paper presents a systematic mapping literature review about the mobile robotics competitions that took place over the last few decades in order to obtain an overview of the main objectives, target public, challenges, technologies used and final application area to show how these competitions have been contributing to education. In the review we found 673 papers from 5 different databases and at the end of the process, 75 papers were classified to extract all the relevant information using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method. More than 50 mobile robotics competitions were found and it was possible to analyze most of the competitions in detail in order to answer the research questions, finding the main goals, target public, challenges, technologies and application area, mainly in education.
Plate Load Tests on an Unsaturated Sand–Kaolin Mixture with Varying Water Table
Authors: Tang Yi , Chen Chenghao , Qian Bin , Ren Jie , Guan Yunfei
Published: March 2022

Clayey sand is widely distributed and commonly encountered in geotechnical engineering practice. To understand its bearing capacity behavior under unsaturated conditions, plate load tests are performed on sand–kaolin mixture samples with varying water tables. The distributions of suction and volumetric water content with depth are measured by vibrating wire piezometers and soil moisture sensors, respectively. It is shown by the test results that the bearing capacity increases when the water table in the soil sample drops. The influence of suction on the bearing capacity is found to be dependent on the height of the water table and the hydraulic loading history of the soil sample. The plate load test results are interpreted using bearing capacity equations. Good agreement is obtained between measured and calculated bearing capacities. This study provides a simple method to estimate the bearing capacity of in situ unsaturated soil foundations.
Underwater Image Enhancement Based on Histogram-Equalization Approximation Using Physics-Based Dichromatic Modeling
Authors: Peng Yan-Tsung , Chen Yen-Rong , Chen Zihao , Wang Jung-Hua , Huang Shih-Chia
Published: March 2022

This work proposes to develop an underwater image enhancement method based on histogram-equalization (HE) approximation using physics-based dichromatic modeling (PDM). Images captured underwater usually suffer from low contrast and color distortions due to light scattering and attenuation. The PDM describes the image formation process, which can be used to restore nature-degraded images, such as underwater images. However, it does not assure that the restored images have good contrast. Thus, we propose approximating the conventional HE based on the PDM to recover the color distortions of underwater images and enhance their contrast through convex optimization. Experimental results demonstrate the proposed method performs favorably against state-of-the-art underwater image restoration approaches.
Toward Dependable Internet of Medical Things: IEEE 802.15.6 Ultra-Wideband Physical Layer Utilizing Superorthogonal Convolutional Code
Authors: Takabayashi Kento , Tanaka Hirokazu , Sakakibara Katsumi
Published: March 2022

Wireless body area networks (WBANs) are attracting attention as a very important technology for realizing an Internet of Medical Things (IoMT). IEEE 802.15.6 is well known as one of the international standards for WBANs for the IoMT. This article proposes the combination of the IEEE 802.15.6 ultra-wideband (UWB) physical layer (PHY) with a super orthogonal convolutional code (SOOC) and evaluates its performance as a dependable WBAN. Numerical results show that sufficient dependability cannot be obtained with the error-correcting code specified in IEEE 802.15.6 when applying the single pulse option, while both high energy efficiency and dependability can be obtained by applying an SOCC. In addition, it is confirmed that higher dependability can be obtained by combining an SOCC with a Reed–Solomon (RS) code with a coding rate that is almost the same as the error correction code specified in the standard. Furthermore, the results indicate that high dependability and energy efficiency can be obtained by adjusting the SOCC coding rate and UWB PHY parameters, even in the burst pulse option. The SOCC-applied UWB PHY of this research satisfies the high requirements of the IoMT.
Parenting Challenges and Opportunities among Families Living in Poverty
Authors: Beasley Lana O. , Jespersen Jens E. , Morris Amanda S. , Farra Aisha , Hays-Grudo Jennifer
Published: March 2022

Poverty-related stressors have been found to impact parenting behaviors which can result in adverse outcomes for children. The current qualitative study focused on understanding the challenges of caregivers (N = 70) living in poverty. The sample was diverse and included mothers, fathers, and grandparents raising grandchildren. Stories of caregivers were gathered to improve the understanding of families living in poverty in an effort to work towards changing how our world supports families that are vulnerable. Results indicate that families experiencing poverty and related risk factors experience challenges in the realm of child safety, education, and racism/prejudice. Families also discussed ways to improve their environment which included increased financial resources, increased access to high-quality healthcare and childcare, and positive environmental change. Note that the current study outlines the complexity of parenting in poverty and that associated challenges are intertwined. Recommendations are made to address systemic barriers at the individual and community level in an effort to better support caregivers experiencing adversity and parenting in the 21st century.
Effects of Different Types of Exercise on Kidney Diseases
Authors: Arazi Hamid , Mohabbat Majid , Saidie Payam , Falahati Akram , Suzuki Katsuhiko
Published: March 2022

The effects of exercise on kidney function have been studied for more than three decades. One of the most common health issues among patients with chronic kidney disease (CKD) is a lack of physical activity, which leads to a low exercise capacity in these patients. The majority of maintenance hemodialysis (MHD) patients do not exercise at all. At each stage of dialysis, patients lose 10–12 g of their amino acids through blood sampling. Dialysis also leads to increased cortisol and circadian rhythm sleep disorders in hemodialysis (HD) patients. Studies have also reported higher C-reactive protein levels in HD patients, which causes arterial stiffness. Exercise has a variety of health benefits in these patients, including improved blood pressure control, better sleep, higher physical function, and reduced anxiety and depression. On the other hand, it should be noted that intense exercise has the potential to progress KD, especially when conducted in hot weather with dehydration. This review aimed to investigate the effects of different types of exercise on kidney disease and provide exercise guidelines. In conclusion, moderate-intensity and long-term exercise (for at least a 6-month period), with consideration of the principles of exercise (individualization, intensity, time, etc.), can be used as an adjunctive treatment strategy in patients undergoing dialysis or kidney transplantation.
Validation of the Perceptions of Inclusion Questionnaire including PE Teachers’ Opinion as Part of an Innovative Use of the Tool
Authors: Zwierzchowska Anna , Kostorz Karolina , Rosołek Barbara , Tomińska-Conte Edyta
Published: March 2022

The aim of the study was to validate the Polish version of the Perceptions of Inclusion Questionnaire (PIQ) for the first time. The analysis included not only pupils, parents/legal guardians, and teachers but also physical education teachers, which is an innovative solution proposed by the authors. The sample consisted of 448 respondents. Confirmatory factor analysis showed that the tested model corresponding to the construct proposed by the PIQ authors did not have good fit indicators (χ2/df = 7.73; CFI = 0.855; SRMR = 0.103; RMSEA = 0.123). The three-factor structure of the questionnaire was confirmed by the eigenvalues obtained for each extracted factor based on the Kaiser criterion and the scree plot based on the Cattella criterion. All three factors showed satisfactory levels of reliability (Cronbach’s alpha > 0.7). The conditions of convergent and discriminant validity for the construct were confirmed. However, Horn’s parallel analysis showed that the optimal number of factors is 4. Therefore, the research presented here should constitute a starting point for further analyses. It is recommended that research teams should conduct further scientific exploration of the PIQ tool in four perspectives, i.e., including PE teachers’ opinions.
Topic Modeling for Hiking Trail Online Reviews: Analysis of the Mutianyu Great Wall
Authors: Shang Ziye , Luo Jian Ming
Published: March 2022

Hiking is now one of the most popular activities amongst adventure travelers. Although recent studies have highlighted the differences between Chinese adventure tourists and their international counterparts, few studies have comprehensively explored the differences in hikers’ interests and concerns for experience elements between these two groups. Topic modeling is adopted for an analysis of the online reviews of the Mutianyu Great Wall to identify attributes influencing hikers’ experiences and behavior. Using a large-scale review dataset, the latent Dirichlet allocation (LDA) technique was applied to construct a comprehensive list of the topics posted by hikers. The findings revealed that Chinese and non-Chinese hikers have common concerns regarding the degree of challenges, scenery, tour services and crowding during hiking. Nevertheless, their perceptions of cultural resources are presented in a different way. These findings are beneficial for understanding the similarities and differences between Chinese and non-Chinese hikers’ perceptions, in addition to improving domestic and international markets’ management and marketing strategies.
Responsible Marketing in the Traffic Light Labeling of Food Products in Ecuador: Perceptions of Cuenca Consumers
Authors: Cordero-Ahiman Otilia Vanessa , Vanegas Jorge Leonardo , Fernández-Lucero Cecilia Alexandra , Torres-Torres Daniela Fernanda , Ayaviri-Nina Víctor Dante , Quispe-Fernández Gabith Miriam
Published: March 2022

Responsible marketing (RM) helps companies make products that improve people’s lives and is in line with public policies that provide a positive impact on society; an example of this is traffic light nutritional labelling (TLNL). Ecuador was a pioneer in Latin America in the mandatory implementation of TLNL. For this reason, this research aimed to analyze RM in the TLNL of food products from the perception of consumers in the urban area of Cuenca, Ecuador. Specifically, the research aimed to answer: What is the degree of consumer understanding of TLNL in the urban area of Cuenca, Ecuador? A cross-sectional investigation was undertaken, with 384 surveys conducted. A chi-square test (χ2) was performed, which showed the relationship between variables reflecting RM knowledge and the understanding and use of TLNL. An ordinal logit model (OLM) was applied, showing that the variables of education, knowledge of labeling, and knowledge of marketing were associated with a greater probability of having some level of understanding of TLNL. Finally, a binomial logit model (BLM) revealed that the variables of income level, knowledge of TLNL, illnesses, confidence in TLNL, the influence of COVID-19 on eating habits, and knowledge of marketing were associated with people being more likely to use TLNL. RM is present in the TLNL through the clear and understandable dissemination of information.
The Mechanism of Forming the Strategic Potential of an Enterprise in a Circular Economy
Authors: Kuzior Aleksandra , Arefieva Olena , Poberezhna Zarina , Ihumentsev Oleksiy
Published: March 2022

In the framework of this study, significant features of the formation of the strategic potential of the enterprise in a circular economy are identified. The characteristics and elements of the strategic potential of the enterprise, which can ensure its integrity and continuity of operations, are highlighted. The authors conducted and analyzed a theoretical review of the concept of the “circular economy” and its impact on business and resource conservation and environmental protection. The conditions for the transition to a circular economy at the macro level are formed. The key stages of ensuring the strategic potential of the enterprise, taking into account the internal and external environmental factors, are highlighted. The authors forecast the volume and dynamics of waste until 2027 using the Cobb–Douglas function. The mechanism of the formation of the strategic potential of the enterprise in the conditions of a circular economy is offered. This mechanism provides for the potential compliance with the strategic goals of the enterprise, as well as the rationality and balance of structural elements. Assessing the compliance of strategic potential with the developed strategy allows decisions to be made on the implementation of measures to meet the objectives of the enterprise, or to search for opportunities and reserves to improve its level. A set of measures aimed at the effective implementation of the proposed mechanism and the results of resource-efficient production is developed.
“Everything Is Changing, but I Am Not Alone”: Nurses’ Perceptions of Social Support during COVID-19
Authors: Sahay Surabhi , Wei Wan
Published: March 2022

COVID-19 has created numerous stressors for nurses, which have impacted their work, self-efficacy, and wellness. Social support helps manage stress and burnout. Through 24 semi-structured interviews, the current article explores the perspectives of nurses who worked with COVID-19 patients in the initial days of the pandemic in the United States. This study unpacks the types and sources of social support nurses sought during this time, especially as they faced significant burnout. Through thematic analysis, the current study found that these nurses interacted with the crisis situation to evaluate their social support needs and the plausibility of fulfilling these needs. They focused on the support that was available or at least perceived to be available and let go of certain needs that could not plausibly be addressed in that moment. Peer-to-peer support was critical during this process, and nurses avoided sharing concerns with their families as they enacted protective buffering. The findings also highlight the complex and dynamic nature of social support as nurses interact with their peers and evaluate the support they receive. Peers helped with haptic support like providing hugs to coworkers, and at times even became surrogates for coworkers’ family members as they participated in communal coping. Organizational support was critical for sharing information centrally with nurses and for organizational sustainability.
Experimental Francis Turbine Cavitation Performances of a Hydro-Energy Plant
Authors: Su Wen-Tao , Zhao Wei , Binama Maxime , Zhao Yue , Huang Jian-Ying , Chen Xue-Ren
Published: March 2022

An investigation is conducted on the Francis turbine’s cavitation characteristics and its influence on system hydraulic stability using two experimental methods, namely the flow visualization and acoustic emission methods. The investigated turbine is of Francis type with a 15-blade runner and has a specific speed of 202 rpm and a rated head of 30 m. Having tested the machine under a wide range of cavitation conditions, the gap cavitation is the earliest to develop as the cavitation coefficient gradually decreases and has no obvious effect on the machine’s external performance characteristics. The airfoil cavitation follows and causes the increase and decrease in machine flow rate and head, respectively, showing its drag reduction effect, where, at the same time, the pressure pulsation amplitude gets to its peak value. There is also the formation of constant cavitation zones and the involvement of an unsteady surge close to the wall of the draft tube’s cone. Pushing the cavitation coefficient to even lower values, there is the formation of an annular cavitation zone, accompanied by a sharp drop in cone pressure pulsation amplitudes while the former drag reduction effect disappears. The trend of noise is basically the same as that of pressure fluctuation, which confirms its trustworthiness when it comes to cavitation occurrence detection within Francis turbines.
Prioritizing Indicators for Sustainability Assessment in Manufacturing Process: An Integrated Approach
Authors: Swarnakar Vikas , Singh Amit Raj , Antony Jiju , Jayaraman Raja , Tiwari Anil Kr , Rathi Rajeev , Cudney Elizabeth
Published: March 2022

Sustainable manufacturing has renewed attention among researchers to address various sustainability challenges in manufacturing industries. Sustainability assessments of manufacturing organizations help minimize the negative environmental impact and enhance reputation among public and regulatory agencies. To assess the sustainability of the manufacturing process; it is indispensable to investigate the structured set of triple bottom line (3BL) indicators. Moreover, there is no comprehensive and structured set of 3BL indicators that can effectively assess the sustainability of any organization’s manufacturing process. This research aims to identify and prioritize experts’ consensus structured set of 3BL indicators. The 3BL indicators were identified through an open-ended questionnaire. The prioritization was performed through the Best-Worst Scaling (BWS) approach. Further, Multi-Criteria Decision Analysis (MCDA) method was utilized to draw the consensus ranking of sustainability indicators in manufacturing. The findings indicated that the release of greenhouse/harmful gas is the best indicator in the perspective of environmental criteria followed by the rate of contribution to society and operational cost are the most important critical indicator in the case of social and economic sustainability criteria. The outcome of the present study will facilitate researchers and practitioners in developing suitable readiness and operational plans for the sustainability assessment of the manufacturing process.
Populism, Austerity and Governance for Sustainable Development in Troubled Times: Introduction to Special Issue
Authors: Baker Susan , Quinn Matthew J.
Published: March 2022

The sustainable development imaginary was built on a belief in co-operation, collaboration and consensus building and requires governance approaches that rely upon the values of a liberal, pluralistic, tolerant, and democratic society. Much scholarship assumed that the European Post-War, welfare, democratic order, with its emerging educated classes, would steadily progress towards an ever more refined and articulated version of these governance values. However, that governance imaginary has become increasingly deradicalised, focused instead on economic efficiency and technocracy. Our current ‘troubled times’ have now seen the rise of right populism and the imposition of austerity policies in Europe. Against this background, six key characteristics of sustainable development are examined through a governance lens—limits to growth, equity, inclusion, reflexivity, participation, and international solidarity—showing how right populism and austerity have further reshaped ideas about how to govern for sustainable development. Right populism and austerity have constrained both the narratives and tools available, while shrinking the political space for co-operation, reflection, and learning, poorly reflecting the governance values thought necessary to achieve an equitable and environmentally sustainable future. This has been further seen in the contested governance of the COVID-19 pandemic and the strategies designed to ensure post-Coronavirus (COVID-19) pandemic economic recovery.
Research on Optimal Operation Model of Virtual Electric Power Plant Considering Net-Zero Carbon Emission
Authors: Wu Yungao , Wu Jing , De Gejirifu , Fan Wei
Published: March 2022

“World Energy Outlook 2021” has mentioned the fact that with the current pledges announced, the 2050 net-zero carbon emission target would not be realized. To further improve energy efficiency, energy integration will be important. Therefore, this paper introduced virtual power plant (VPP) and power to gas (P2G) technology to analyze the improvement of energy integration. Firstly, the structure of VPP connected with P2G is proposed, and the physical output model is constructed. Secondly, combined with carbon emission and economic operation objectives, a multi-objective operation optimization model of VPP considering electrical interconnection is constructed, and the solution idea of the model is put forward. Finally, through the case study, the contribution of P2G, DR and GST is proven. With DR, P2G involved in VPP, the goal of carbon emission reduction can be achieved. In addition, the example also proves that carbon trading has a positive effect on energy efficiency and generation uncertainty improving.
STxB as an Antigen Delivery Tool for Mucosal Vaccination
Authors: Tartour Eric , Johannes Ludger
Published: March 2022

Immunotherapy against cancer and infectious disease holds the promise of high efficacy with minor side effects. Mucosal vaccines to protect against tumors or infections disease agents that affect the upper airways or the lung are still lacking, however. One mucosal vaccine candidate is the B-subunit of Shiga toxin, STxB. In this review, we compare STxB to other immunotherapy vectors. STxB is a non-toxic protein that binds to a glycosylated lipid, termed globotriaosylceramide (Gb3), which is preferentially expressed by dendritic cells. We review the use of STxB for the cross-presentation of tumor or viral antigens in a MHC class I-restricted manner to induce humoral immunity against these antigens in addition to polyfunctional and persistent CD4+ and CD8+ T lymphocytes capable of protecting against viral infection or tumor growth. Other literature will be summarized that documents a powerful induction of mucosal IgA and resident memory CD8+ T cells against mucosal tumors specifically when STxB-antigen conjugates are administered via the nasal route. It will also be pointed out how STxB-based vaccines have been shown in preclinical cancer models to synergize with other therapeutic modalities (immune checkpoint inhibitors, anti-angiogenic therapy, radiotherapy). Finally, we will discuss how molecular aspects such as low immunogenicity, cross-species conservation of Gb3 expression, and lack of toxicity contribute to the competitive positioning of STxB among the different DC targeting approaches. STxB thereby appears as an original and innovative tool for the development of mucosal vaccines in infectious diseases and cancer.
Known and Potential Invertebrate Vectors of Raspberry Viruses
Authors: Tan Jiunn Luh , Trandem Nina , Fránová Jana , Hamborg Zhibo , Blystad Dag-Ragnar , Zemek Rostislav
Published: March 2022

The estimated global production of raspberry from year 2016 to 2020 averaged 846,515 tons. The most common cultivated Rubus spp. is European red raspberry (Rubus idaeus L. subsp. idaeus). Often cultivated for its high nutritional value, the red raspberry (Rubus idaeus) is susceptible to multiple viruses that lead to yield loss. These viruses are transmitted through different mechanisms, of which one is invertebrate vectors. Aphids and nematodes are known to be vectors of specific raspberry viruses. However, there are still other potential raspberry virus vectors that are not well-studied. This review aimed to provide an overview of studies related to this topic. All the known invertebrates feeding on raspberry were summarized. Eight species of aphids and seven species of plant-parasitic nematodes were the only proven raspberry virus vectors. In addition, the eriophyid mite, Phyllocoptes gracilis, has been suggested as the natural vector of raspberry leaf blotch virus based on the current available evidence. Interactions between vector and non-vector herbivore may promote the spread of raspberry viruses. As a conclusion, there are still multiple aspects of this topic that require further studies to get a better understanding of the interactions among the viral pathogens, invertebrate vectors, and non-vectors in the raspberry agroecosystem. Eventually, this will assist in development of better pest management strategies.
Generation of Premature Termination Codon (PTC)-Harboring Pseudorabies Virus (PRV) via Genetic Code Expansion Technology
Authors: Wang Tong-Yun , Sang Guo-Ju , Wang Qian , Leng Chao-Liang , Tian Zhi-Jun , Peng Jin-Mei , Wang Shu-Jie , Sun Ming-Xia , Meng Fan-Dan , Zheng Hao , Cai Xue-Hui , Tang Yan-Dong
Published: March 2022

Despite many efforts and diverse approaches, developing an effective herpesvirus vaccine remains a great challenge. Traditional inactivated and live-attenuated vaccines always raise efficacy or safety concerns. This study used Pseudorabies virus (PRV), a swine herpes virus, as a model. We attempted to develop a live but replication-incompetent PRV by genetic code expansion (GCE) technology. Premature termination codon (PTC) harboring PRV was successfully rescued in the presence of orthogonal system MbpylRS/tRNAPyl pair and unnatural amino acids (UAA). However, UAA incorporating efficacy seemed extremely low in our engineered PRV PTC virus. Furthermore, we failed to establish a stable transgenic cell line containing orthogonal translation machinery for PTC virus replication, and we demonstrated that orthogonal tRNAPyl is a key limiting factor. This study is the first to demonstrate that orthogonal translation system-mediated amber codon suppression strategy could precisely control PRV-PTC engineered virus replication. To our knowledge, this is the first reported PTC herpesvirus generated by GCE technology. Our work provides a proof-of-concept for generating UAAs-controlled PRV-PTC virus, which can be used as a safe and effective vaccine.
COVID-19 at a Glance: An Up-to-Date Overview on Variants, Drug Design and Therapies
Authors: Iacopetta Domenico , Ceramella Jessica , Catalano Alessia , Saturnino Carmela , Pellegrino Michele , Mariconda Annaluisa , Longo Pasquale , Sinicropi Maria Stefania , Aquaro Stefano
Published: March 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a member of the Coronavirus family which caused the worldwide pandemic of human respiratory illness coronavirus disease 2019 (COVID-19). Presumably emerging at the end of 2019, it poses a severe threat to public health and safety, with a high incidence of transmission, predominately through aerosols and/or direct contact with infected surfaces. In 2020, the search for vaccines began, leading to the obtaining of, to date, about twenty COVID-19 vaccines approved for use in at least one country. However, COVID-19 continues to spread and new genetic mutations and variants have been discovered, requiring pharmacological treatments. The most common therapies for COVID-19 are represented by antiviral and antimalarial agents, antibiotics, immunomodulators, angiotensin II receptor blockers, bradykinin B2 receptor antagonists and corticosteroids. In addition, nutraceuticals, vitamins D and C, omega-3 fatty acids and probiotics are under study. Finally, drug repositioning, which concerns the investigation of existing drugs for new therapeutic target indications, has been widely proposed in the literature for COVID-19 therapies. Considering the importance of this ongoing global public health emergency, this review aims to offer a synthetic up-to-date overview regarding diagnoses, variants and vaccines for COVID-19, with particular attention paid to the adopted treatments.
I226R Protein of African Swine Fever Virus Is a Suppressor of Innate Antiviral Responses
Authors: Hong Jinxuan , Chi Xiaojuan , Yuan Xu , Wen Faxin , Rai Kul Raj , Wu Lei , Song Zhongbao , Wang Song , Guo Guijie , Chen Ji-Long
Published: March 2022

African swine fever is one of the most devastating swine diseases caused by African swine fever virus (ASFV). Although ASFV encodes more than 160 viral proteins, the implication of a majority of ASFV proteins in regulating host immunity is yet to be explored, and the mechanisms of immune evasion by ASFV proteins are largely unknown. Here, we report that the I226R protein of ASFV significantly suppressed innate immune responses. The ectopic expression of ASFV I226R in 293T cells significantly inhibited the activation of interferon-stimulated response element promoters triggered by Sendai virus (SeV), poly(I:C), or cyclic GMP-AMP synthase (cGAS)/STING. The I226R protein caused a significant decrease in the expression of interferons and interferon-stimulating genes in cells infected with SeV. Similar results were obtained from experiments using I226R-overexpressed PK15 and 3D4/21 cells stimulated with vesicular stomatitis virus. We observed that I226R inhibited the activation of both nuclear factor-kappa B (NF-κB) and interferon regulatory factor 3 (IRF3). Furthermore, it was shown that overexpression of I226R suppressed IRF3 activation and caused the degradation of NF-κB essential modulator (NEMO) protein. The I226R-induced NEMO degradation could be prevented by treatment with MG132, a proteasome inhibitor. Together, these results reveal that the ASFV I226R protein impairs antiviral responses, likely through multiple mechanisms including the suppression of NF-κB and IRF3 activation, to counteract innate immune responses during the viral infection.
Real-Life Safety Profile of the 9-Valent HPV Vaccine Based on Data from the Puglia Region of Southern Italy
Authors: Di Lorenzo Antonio , Berardi Paola , Martinelli Andrea , Bianchi Francesco Paolo , Tafuri Silvio , Stefanizzi Pasquale
Published: March 2022

Human Papillomavirus (HPV) is responsible for epithelial lesions and cancers in both males and females. The latest licensed HPV vaccine is Gardasil-9®, a 9-valent HPV vaccine which is effective not only against the high-risk HPV types, but also against the ones responsible for non-cancerous lesions. This report describes adverse events following Gardasil-9® administration reported in Puglia, southern Italy, from January 2018 to November 2021. This is a retrospective observational study. Data about the adverse events following immunization (AEFIs) with Gardasil-9® were collected from the Italian Drug Authority database. AEFIs were classified as serious or non-serious accordingly to World Health Organization guidelines, and serious ones underwent causality assessment. During the study period, 266,647 doses of 9vHPVv were administered in Puglia and 22 AEFIs were reported, with a reporting rate (RR) of 8.25 per 100,000 doses. The most reported symptoms were neurological ones (7/22). A total of 5 (22.7%) AEFIs were classified as serious, and 2 of these led to the patient’s hospitalization. In one case, permanent impairment occurred. Following causality assessment, only 2 out of 5 serious AEFIs were deemed to be consistently associated with the vaccination (RR: 0.750 per 100,000 doses). The data gathered in our study are similar to the pre-licensure evidence as far as the nature of the AEFIs is concerned. The reporting rate, though, is far lower than the ones described in clinical trials, likely due to the different approach to data collection: in our study, data were gathered via passive surveillance, while pre-marketing studies generally employ active calls for this purpose. Gardasil-9®’s safety profile appears to be favorable, with a low rate of serious adverse events and a risk/benefits ratio pending for the latter.
Knowledge and Attitudes Concerning Herpes Zoster among People with COPD: An Interventional Survey Study
Authors: Yawn Barbara P. , Merrill Debora D. , Martinez Sergio , Callen Elisabeth , Cotton Janice , Williams Dennis , Loskutova Natalia Y.
Published: March 2022

Herpes zoster (HZ) is common in older adults with conditions such as chronic obstructive pulmonary disease (COPD). Effective prevention is available through vaccination, but HZ vaccine uptake remains incomplete. Using an online survey of people with self-reported COPD, ShiPPS assessed HZ risk awareness, HZ vaccine use and barriers, and the impact of an HZ educational video on vaccine intent. USA members of the COPD Foundation’s Patient-Powered Research Network aged >50 years were surveyed in fall 2020. The responses were analyzed using descriptive and comparative statistics. Of the 735 respondents (59.6% female, mean age 68.5 years), 192 (26.1%) reported previous HZ, of whom 49 (25.5%) reported increased COPD symptoms during HZ episodes. Most participants (94.0%) knew of HZ vaccines, but only 33.1% reported receiving the Advisory Committee on Immunization Practices-preferred recombinant HZ vaccination. The recall of receiving HZ vaccine recommendations differed by the site attended: 68.8% primary care, 26.6% pulmonology offices. Most (74.7%) were unaware that COPD increases HZ risk. Among unvaccinated participants, interest in getting the HZ vaccine increased from 32.0% to 73.5% after watching the video. These results highlight the need for people with COPD to receive further HZ education, such as the five-minute video, and HZ vaccine recommendations from healthcare professionals.
Willingness to Take the Booster Vaccine in a Nationally Representative Sample of Danes
Authors: Jørgensen Frederik Juhl , Nielsen Louise Halberg , Petersen Michael Bang
Published: March 2022

We estimate the willingness to take the booster dose in a representative sample of Danes. We estimate an overall willingness in the adult Danish population of about 87 percent and a willingness of about 95.5 percent among primary vaccine takers. Moreover, we show that these percentages are significantly lower among younger populations, as well as among groups who do not see COVID-19 as a threat to society, those who do not feel that they have the ability to follow recommendations (‘self-efficacy’), those who do not perceive the advice of the health authorities as effective against disease spread (‘response efficacy’), and those who feel that the costs of following recommendations are high (‘response cost’).
In Vitro Anthelmintic Activity of Isatis tinctoria Extracts against Ewes’ Gastrointestinal Nematodes (GINs), a Possible Application for Animal Welfare
Authors: Ragusa Monica , Miceli Natalizia , Piras Cristian , Bosco Antonio , Castagna Fabio , Rinaldi Laura , Musella Vincenzo , Taviano Maria Fernanda , Britti Domenico
Published: March 2022

Sheep gastrointestinal nematode (GIN) infestation represents a limiting factor for sheep farming and milk production in Italy. The development of anthelmintic resistance to conventionally used drugs suggests the path towards the use of natural remedies as a possible alternative. The purpose of this study is to evaluate the in vitro anthelmintic efficacy of the hydroalcoholic extracts of basal leaves (It-BL), cauline leaves (It-CL) and flowers (It-F) of Isatis tinctoria (Brassicaceae), a spontaneous Sicilian species renowned as an important source of bioactive compounds. The dry extracts of the different parts of the plant were tested using the egg hatch test (EHT) in vitro to verify the efficacy against ovine GIN at different concentrations (1.00, 0.5, 0.25, 0.125 mg/mL). Thiabendazole and deionized water were used as positive and negative controls, respectively. The results obtained from EHT indicated that all the I. tinctoria extracts were highly effective (p < 0.0001) in inhibiting egg hatching within 48 h of exposure. The in vitro inhibitory effect was never less than 84% in all doses tested, and it was only slightly lower than the standard drug thiabendazole (95.6%). The current study documents the anthelmintic activity of I. tinctoria against sheep’s GIN, suggesting its application as alternative natural method to limit the use of antiparasitic drugs.
Sugar Beet Processing Wastewater Treatment by Microalgae through Biosorption
Authors: Khakimova Nadiia , Maravić Nikola , Davidović Petar , Blagojević Dajana , Bečelić-Tomin Milena , Simeunović Jelica , Pešić Vesna , Šereš Zita , Mandić Anamarija , Pojić Milica , Mišan Aleksandra
Published: March 2022

The aim of this study was to investigate the potential of environmental pollution reduction of sugar beet processing factory wastewater by the biorefinery approach and integration of microalgae biomass production. In the present study, Chlorella vulgaris was cultivated in wastewater collected from a sugar beet processing factory at the beginning and at the end of a sugar plant campaign in an aerobic bioreactor on a laboratory scale under controlled conditions, with an air flow of 0.4 L/min, a temperature of 26 °C, and pH = 8. Microalgae showed effective nutrient remediation from wastewater. During wastewater treatment, chemical oxygen demand (COD) and biological oxygen demand (BOD) removal efficiency was 93.7% and 98.1%, respectively; total organic carbon (TOC) content decreased by 95.7%. Nitrites and nitrates decreased by 96%, while the biggest decrease in metal ions was achieved for Ca and Mn (82.7% and 97.6%, respectively). The findings of this study suggest that coupling microalgae cultivation and wastewater treatment has a lot of potential for reducing contamination through biosorption, while also providing environmental advantages.
The WHO Guidelines for Safe Wastewater Use in Agriculture: A Review of Implementation Challenges and Possible Solutions in the Global South
Authors: Drechsel Pay , Qadir Manzoor , Galibourg David
Published: March 2022

Globally, the use of untreated, often diluted, or partly treated wastewater in agriculture covers about 30 million ha, far exceeding the area under the planned use of well-treated (reclaimed) wastewater which has been estimated in this paper at around 1.0 million ha. This gap has likely increased over the last decade despite significant investments in treatment capacities, due to the even larger increases in population, water consumption, and wastewater generation. To minimize the human health risks from unsafe wastewater irrigation, the WHO’s related 2006 guidelines suggest a broader concept than the previous (1989) edition by emphasizing, especially for low-income countries, the importance of risk-reducing practices from ‘farm to fork’. This shift from relying on technical solutions to facilitating and monitoring human behaviour change is, however, challenging. Another challenge concerns local capacities for quantitative risk assessment and the determination of a risk reduction target. Being aware of these challenges, the WHO has invested in a sanitation safety planning manual which has helped to operationalize the rather academic 2006 guidelines, but without addressing key questions, e.g., on how to trigger, support, and sustain the expected behaviour change, as training alone is unlikely to increase the adoption of health-related practices. This review summarizes the perceived challenges and suggests several considerations for further editions or national adaptations of the WHO guidelines.