bluuebunny/crossref_metadata_2025 huggingface dataset:
The Present Issues of Control Automation for Levitation Metal Melting
Authors: Boikov Aleksei , Payor Vladimir
Published: September 2022

This article is a review of current scientific problems in the field of automation of the electromagnetic levitation melting process control of non-ferrous metals and potential solutions using modern digital technologies. The article describes the technological process of electromagnetic levitation melting as a method of obtaining ultrapure metals and the main problems of the automation of this process taking into account domestic and international experience. Promising approaches to control the position of the melt in the inductor in real time on the basis of vision systems are considered. The main problems and factors preventing the mass introduction of levitation melting in the electromagnetic field to the industry are highlighted. The problem of passing the Curie point by the heated billet and the effect of the billet’s loss of magnetism on the vibrational circuit of the installation and the temperature of the inductor are also considered. The article also reflects key areas of research development in the field of levitation melting, including: optimization of energy costs, stabilization of the position of the melt in the inductor, predictive process control, and scaling of levitation melting units. The concept of a digital twin based on a numerical model as a component of an automatic process control system for the implementation of inductor control and prediction of process parameters of the melt is presented. The possibility of using vision for visual control of the melt position in the inductor based on video images for its further stabilization in the inductor and increasing the accuracy of numerical simulation results by specifying the real geometry of the melt in parallel with the calculation of the model itself is considered.
Project Manager Competencies for Dealing with Socio-Technical Complexity: A Grounded Theory Construction
Authors: Ahmadi Eftekhari Navid , Mani Saba , Bakhshi Javad , Mani Sahar
Published: September 2022

An ongoing question is what constitutes the characteristics of a project manager. This is the subject of many studies. The characteristics, skills, abilities and knowledge of project managers—essential factors in a project’s success—describe their level of competency. This study aims to assess the relationship between project manager competencies and project complexity in the information technology (IT) sector. In total, 21 semi-structured interviews were conducted with senior practitioners associated with complex IT projects in the private and public sectors. All transcripts were analysed through grounded theory and content analysis, with experts approving the results. Our study identified 41 competencies within project complexity, with these grouped under the following 10 dimensions: project management (PM) knowledge; management skills; interpersonal skills and attributes; professionalism; expertise; emotional skills; contextual skills; influencing skills; team working; and cognitive skills. According to this research, leadership is the core competency of a project manager, while project management knowledge is the most essential of these competency dimensions. This study’s findings can assist both academics and practitioners in simplifying the complexity of projects and helping to achieve a project’s objectives.
Oligonucleotide-Based Therapies for Chronic HBV Infection: A Primer on Biochemistry, Mechanisms and Antiviral Effects
Authors: Vaillant Andrew
Published: September 2022

Three types of oligonucleotide-based medicines are under clinical development for the treatment of chronic HBV infection. Antisense oligonucleotides (ASOs) and synthetic interfering RNA (siRNA) are designed to degrade HBV mRNA, and nucleic acid polymers (NAPs) stop the assembly and secretion of HBV subviral particles. Extensive clinical development of ASOs and siRNA for a variety of liver diseases has established a solid understanding of their pharmacodynamics, accumulation in different tissue types in the liver, pharmacological effects, off-target effects and how chemical modifications and delivery approaches affect these parameters. These effects are highly conserved for all ASO and siRNA used in human studies to date. The clinical assessment of several ASO and siRNA compounds in chronic HBV infection in recent years is complicated by the different delivery approaches used. Moreover, these assessments have not considered the large clinical database of ASO/siRNA function in other liver diseases and known off target effects in other viral infections. The goal of this review is to summarize the current understanding of ASO/siRNA/NAP pharmacology and integrate these concepts into current clinical results for these compounds in the treatment of chronic HBV infection.
Norovirus Infection in Young Nicaraguan Children Induces Durable and Genotype-Specific Antibody Immunity
Authors: Brewer-Jensen Paul D. , Reyes Yaoska , Becker-Dreps Sylvia , González Fredman , Mallory Michael L. , Gutiérrez Lester , Zepeda Omar , Centeno Edwing , Vielot Nadja , Diez-Valcarce Marta , Vinjé Jan , Baric Ralph , Lindesmith Lisa C. , Bucardo Filemon
Published: September 2022

There are significant challenges to the development of a pediatric norovirus vaccine, mainly due to the antigenic diversity among strains infecting young children. Characterizing human norovirus serotypes and understanding norovirus immunity in naïve children would provide key information for designing rational vaccine platforms. In this study, 26 Nicaraguan children experiencing their first norovirus acute gastroenteritis (AGE) episode during the first 18 months of life were investigated. We used a surrogate neutralization assay that measured antibodies blocking the binding of 13 different norovirus virus-like particles (VLPs) to histo-blood group antigens (HBGAs) in pre- and post-infection sera. To assess for asymptomatic norovirus infections, stools from asymptomatic children were collected monthly, screened for norovirus by RT-qPCR and genotyped by sequencing. Seroconversion of an HBGA-blocking antibody matched the infecting genotype in 25 (96%) of the 26 children. A subset of 13 (50%) and 4 (15%) of the 26 children experienced monotypic GII and GI seroconversion, respectively, strongly suggesting a type-specific response in naïve children, and 9 (35%) showed multitypic seroconversion. The most frequent pairing in multitypic seroconversion (8/12) were GII.4 Sydney and GII.12 noroviruses, both co-circulating at the time. Blocking antibody titers to these two genotypes did not correlate with each other, suggesting multiple exposure rather than cross-reactivity between genotypes. In addition, GII titers remained consistent for at least 19 months post-infection, demonstrating durable immunity. In conclusion, the first natural norovirus gastroenteritis episodes in these young children were dominated by a limited number of genotypes and induced responses of antibodies blocking binding of norovirus VLPs in a genotype-specific manner, suggesting that an effective pediatric norovirus vaccine likely needs to be multivalent and include globally dominant genotypes. The duration of protection from natural infections provides optimism for pediatric norovirus vaccines administered early in life.
The First Human Vulvar Intraepithelial Neoplasia Cell Line with Naturally Infected Episomal HPV18 Genome
Authors: Wu Ming , Zhang Xiu , Kang Yiyi , Zhu Yaqi , Su Zhaoyu , Liu Jun , Zhang Wei , Chen Hong , Li Hui
Published: September 2022

Persistent infection with high-risk HPV leads to cervical cancers and other anogenital cancers and head and neck carcinomas in both men and women. There is no effective drug fortreating HPV infection and HPV-associated carcinomas, largely due to a lack of models of natural HPV infection and the complexity of the HPV life cycle. There are no available cell lines from vulvar, anal, or penile lesions and cancers in the field. In this study, we established the first human cell line from vulvar intraepithelial neoplasia (VIN) with naturally infected HPV18 by conditional reprogramming (CR) method. Our data demonstrated that VIN cells possessed different biological characteristics and diploid karyotypes from HPV18-positive cancer cells (HeLa). Then, we determined that VIN cells contained episomal HPV18 using approaches including the ratio of HPV E2copy/E7copy, rolling cycle amplification, and sequencing. The VIN cells expressed squamous epithelium-specific markers that are different from HeLa cells, a cervical adenocarcinoma cell line. When cultured under 3D air–liquid interface (ALI) system, we observed the expression of both early and late differentiation markers involucrin and filaggrin. Most importantly, we were able to detect the expression of viral late gene L1 in the cornified layer of ALI 3D culture derived from VIN cells, suggesting quite different HPV genomic status from cancer cells. We also observed progeny viral particles under transmission electron microscopy (TEM) in ALI 3D cultures, confirming the episomal HPV18 genome and active viral life cycle in the new cell line. To our knowledge, this is the first human VIN cell line with naturally infected HPV18 genome and provides a valuable model for HPV biology studies, HPV-associated cancer initiation and progression, and drug-screening platforms.
Chemically Modified Bovine β-Lactoglobulin as a Broad-Spectrum Influenza Virus Entry Inhibitor with the Potential to Combat Influenza Outbreaks
Authors: Fu Yuhong , Li Peiyu , Xu Wei , Liu Zezhong , Wang Cong , Wang Qian , Tang Jiayi , Li Weihua , Lu Lu , Jiang Shibo
Published: September 2022

Frequent outbreaks of the highly pathogenic influenza A virus (AIV) infection, together with the lack of broad-spectrum influenza vaccines, call for the development of broad-spectrum prophylactic agents. Previously, 3-hydroxyphthalic anhydride-modified bovine β-lactoglobulin (3HP-β-LG) was proven to be effective against human immunodeficiency virus (HIV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and it has also been used in the clinical control of cervical human papillomavirus (HPV) infections. Here, we show its efficacy in potently inhibiting infection by divergent influenza A and B viruses. Mechanistic studies suggest that 3HP-β-LG binds, possibly through its negatively charged residues, to the receptor-binding domain in the hemagglutinin 1 (HA1) subunit in the HA of the influenza virus, thus inhibiting the attachment of the HA to sialic acid on host cells. The intranasal administration of 3HP-β-LG led to the protection of mice against challenges by influenza A(H1N1)/PR8, A(H3N2), and A(H7N9) viruses. Furthermore, 3HP-β-LG is highly stable when stored at 50 °C for 30 days and it shows excellent safety in vitro and in vivo. Collectively, our findings suggest that 3HP-β-LG could be successfully repurposed as an intranasal prophylactic agent to prevent influenza virus infections during influenza outbreaks.
Aerosol Survival, Disinfection and Formalin Inactivation of Nipah Virus
Authors: Smither Sophie J. , Eastaugh Lin S. , O’Brien Lyn M. , Phelps Amanda L. , Lever Mark S.
Published: September 2022

Nipah virus is a relatively newly discovered emerging virus on the WHO list of priority pathogens which has the potential to cause outbreaks with high fatality rates. Whilst progress is being made in the development of animal models for evaluating vaccines and therapies, some of the more fundamental data on Nipah virus are lacking. We performed studies to generate novel information on the aerosol survival of Nipah virus and to look at the efficacy of two common disinfectants. We also performed studies to evaluate the inactivation of Nipah virus by using neutral buffered formalin. Nipah virus was relatively stable in a small particle (1–5 µm) aerosol in the dark, with it having a decay rate of 1.46%min−1. Sodium hypochlorite (at 10%) and ethanol (at 80%) reduced the titre of Nipah virus to undetectable levels. Nipah virus that was in tissue culture medium was also inactivated after 24 h in the presence of 10% formalin.
RIOK3 and Its Alternatively Spliced Isoform Have Disparate Roles in the Innate Immune Response to Rift Valley Fever Virus (MP12) Infection
Authors: Bisom Thomas C. , White Luke A. , Lanchy Jean-Marc , Lodmell J. Stephen
Published: September 2022

Rift Valley fever virus (RVFV) is a pathogenic human and livestock RNA virus that poses a significant threat to public health and biosecurity. During RVFV infection, the atypical kinase RIOK3 plays important roles in the innate immune response. Although its exact functions in innate immunity are not completely understood, RIOK3 has been shown to be necessary for mounting an antiviral interferon (IFN) response to RVFV in epithelial cells. Furthermore, after immune stimulation, the splicing pattern for RIOK3 mRNA changes markedly, and RIOK3′s dominant alternatively spliced isoform, RIOK3 X2, exhibits an opposite effect on the IFN response by dampening it. Here, we further investigate the roles of RIOK3 and its spliced isoform in other innate immune responses to RVFV, namely the NFκB-mediated inflammatory response. We find that while RIOK3 is important for negatively regulating this inflammatory pathway, its alternatively spliced isoform, RIOK3 X2, stimulates it. Overall, these data demonstrate that both RIOK3 and its X2 isoform have unique roles in separate innate immune pathways that respond to RVFV infection.
Predicting Egg Passage Adaptations to Design Better Vaccines for the H3N2 Influenza Virus
Authors: Liu Yunsong , Chen Hui , Duan Wenyuan , Zhang Xinyi , He Xionglei , Nielsen Rasmus , Ma Liang , Zhai Weiwei
Published: September 2022

Seasonal H3N2 influenza evolves rapidly, leading to an extremely poor vaccine efficacy. Substitutions employed during vaccine production using embryonated eggs (i.e., egg passage adaptation) contribute to the poor vaccine efficacy (VE), but the evolutionary mechanism remains elusive. Using an unprecedented number of hemagglutinin sequences (n = 89,853), we found that the fitness landscape of passage adaptation is dominated by pervasive epistasis between two leading residues (186 and 194) and multiple other positions. Convergent evolutionary paths driven by strong epistasis explain most of the variation in VE, which has resulted in extremely poor vaccines for the past decade. Leveraging the unique fitness landscape, we developed a novel machine learning model that can predict egg passage substitutions for any candidate vaccine strain before the passage experiment, providing a unique opportunity for the selection of optimal vaccine viruses. Our study presents one of the most comprehensive characterizations of the fitness landscape of a virus and demonstrates that evolutionary trajectories can be harnessed for improved influenza vaccines.
Complete Genome and Molecular Characterization of a New Cyprinid Herpesvirus 2 (CyHV-2) SH-01 Strain Isolated from Cultured Crucian Carp
Authors: Yang Jia , Wen Jinxuan , Xiao Simin , Wei Chang , Yu Fei , Roengjit Patarida , Lu Liqun , Wang Hao
Published: September 2022

Cyprinid herpesvirus 2 (CyHV-2) is a causative factor of herpesviral hematopoietic necrosis (HVHN) in farmed crucian carp (Carassius carassius) and goldfish (Carassius auratus). In this study, we analyzed the genomic characteristics of a new strain, CyHV-2 SH-01, isolated during outbreaks in crucian carp at a local fish farm near Shanghai, China. CyHV-2 SH-01 exhibited a high sensitivity to goldfish and crucian carp in our previous research. The complete genome of SH-01 is 290,428 bp with 154 potential open reading frames (ORFs) and terminal repeat (TR) regions at both ends. Compared to the sequenced genomes of other CyHVs, Carassius auratus herpesvirus (CaHV) and Anguillid herpesvirus 1 (AngHV-1), several variations were found in SH-01, including nucleotide mutations, deletions, and insertions, as well as gene duplications, rearrangements, and horizontal transfers. Overall, the genome of SH-01 shares 99.60% of its identity with that of ST-J1. Genomic collinearity analysis showed that SH-01 has a high degree of collinearity with another three CyHV-2 isolates, and it is generally closely related to CaHV, CyHV-1, and CyHV-3, although it contains many differences in locally collinear blocks (LCBs). The lowest degree of collinearity was found with AngHV-1, despite some homologous LCBs, indicating that they are evolutionarily the most distantly related. The results provide new clues to better understand the CyHV-2 genome through sequencing and sequence mining.
Vaccination Decreases the Infectious Viral Load of Delta Variant SARS-CoV-2 in Asymptomatic Patients
Authors: Plante Jessica A. , Machado Rafael R. G. , Mitchell Brooke M. , Shinde Divya P. , Walker Jordyn , Scharton Dionna , McConnell Allan , Saada Nehad , Liu Jianying , Khan Bilal , Campos Rafael K. , Johnson Bryan A. , Menachery Vineet D. , Levine Corri B. , Ren Ping , McLellan Susan L. F. , Plante Kenneth S. , Weaver Scott C.
Published: September 2022

The Delta variant of SARS-CoV-2 has caused many breakthrough infections in fully vaccinated individuals. While vaccine status did not generally impact the number of viral RNA genome copies in nasopharyngeal swabs of breakthrough patients, as measured by Ct values, it has been previously found to decrease the infectious viral load in symptomatic patients. We quantified the viral RNA, infectious virus, and anti-spike IgA in nasopharyngeal swabs collected from individuals asymptomatically infected with the Delta variant of SARS-CoV-2. Vaccination decreased the infectious viral load, but not the amount of viral RNA. Furthermore, vaccinees with asymptomatic infections had significantly higher levels of anti-spike IgA in their nasal secretions compared to unvaccinated individuals with asymptomatic infections. Thus, vaccination may decrease the transmission risk of Delta, and perhaps other variants, despite not affecting the amount of viral RNA measured in nasopharyngeal swabs.
Lysyl Oxidase-like Protein Recognizes Viral Envelope Proteins and Bacterial Polysaccharides against Pathogen Infection via Induction of Expression of Antimicrobial Peptides
Authors: Lu Peng-Yuan , Niu Guo-Juan , Hong Pan-Pan , Wang Jin-Xing
Published: September 2022

Lysyl oxidases (LOXs) are copper-dependent monoamine oxidases, and they play critical roles in extracellular matrix (ECM) remodeling. The LOX and LOX-like (LOXL) proteins also have a variety of biological functions, such as development and growth regulation, tumor suppression, and cellular senescence. However, the functions of LOXLs containing repeated scavenger receptor cysteine-rich (SRCR) domains in immunity are rarely reported. In this study, we characterized the antiviral and antibacterial functions of a lysyl oxidase-like (LOXL) protein containing tandem SRCR domains in Marsupenaeus japonicus. The mRNA level of LoxL was significantly upregulated in the hemocytes and intestines of shrimp challenged using white spot syndrome virus (WSSV) or bacteria. After the knockdown of LoxL via RNA interference, WSSV replication and bacterial loads were apparently increased, and the survival rate of the shrimp decreased significantly, suggesting that LOXL functions against pathogen infection in shrimp. Mechanistically, LOXL interacted with the envelope proteins of WSSV or with lipopolysaccharide and peptidoglycan from bacteria in shrimp challenged using WSSV or bacteria, and it promoted the expression of a battery of antimicrobial peptides (AMPs) via the induction of Dorsal nuclear translocation against viral and bacterial infection. Moreover, LOXL expression was also positively regulated by Dorsal in the shrimp challenged by pathogens. These results indicate that, by acting as a pattern recognition receptor, LOXL plays vital roles in antiviral and antibacterial innate immunity by enhancing the expression of AMPs in shrimp.
Development and Characterization of a Genetically Stable Infectious Clone for a Genotype I Isolate of Dengue Virus Serotype 1
Authors: Hu Mingyue , Wu Tiantian , Yang Yang , Chen Tongling , Hao Jiawei , Wei Youchuan , Luo Tingrong , Wu De , Li Yi-Ping
Published: September 2022

Dengue virus (DENV) is primarily transmitted by the bite of an infected mosquito of Aedes aegypti and Aedes albopictus, and symptoms caused may range from mild dengue fever to severe dengue hemorrhagic fever and dengue shock syndrome. Reverse genetic system represents a valuable tool for the study of DENV virology, infection, pathogenesis, etc. Here, we generated and characterized an eukaryotic-activated full-length infectious cDNA clone for a DENV serotype 1 (DENV-1) isolate, D19044, collected in 2019. Initially, nearly the full genome was determined by sequencing overlapping RT-PCR products, and was classified to be genotype I DENV-1. D19044 wild-type cDNA clone (D19044_WT) was assembled by four subgenomic fragments, in a specific order, into a low-copy vector downstream the CMV promoter. D19044_WT released the infectious virus at a low level (1.26 × 103 focus forming units per milliliter [FFU/mL]) following plasmid transfection of BHK-21 cells. Further adaptation by consecutive virus passages up to passage 37, and seven amino acid substitutions (7M) were identified from passage-recovered viruses. The addition of 7M (D19044_7M) greatly improved viral titer (7.5 × 104 FFU/mL) in transfected BHK-21 culture, and virus infections in 293T, Huh7.5.1, and C6/36 cells were also efficient. D19044_7M plasmid was genetically stable in transformant bacteria after five transformation-purification cycles, which did not change the capacity of producing infectious virus. Moreover, the D19044_7M virus was inhibited by mycophenolic acid in a dose-dependent manner. In conclusion, we have developed a DNA-launched full-length infectious clone for a genotype I isolate of DENV-1, with genetic stability in transformant bacteria, thus providing a useful tool for the study of DENV-1.
Structural Basis for the Inhibition of Coronaviral Main Proteases by a Benzothiazole-Based Inhibitor
Authors: Hu Xiaohui , Lin Cheng , Xu Qin , Zhou Xuelan , Zeng Pei , McCormick Peter J. , Jiang Haihai , Li Jian , Zhang Jin
Published: September 2022

The ongoing spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused hundreds of millions of cases and millions of victims worldwide with serious consequences to global health and economies. Although many vaccines protecting against SARS-CoV-2 are currently available, constantly emerging new variants necessitate the development of alternative strategies for prevention and treatment of COVID-19. Inhibitors that target the main protease (Mpro) of SARS-CoV-2, an essential enzyme that promotes viral maturation, represent a key class of antivirals. Here, we showed that a peptidomimetic compound with benzothiazolyl ketone as warhead, YH-53, is an effective inhibitor of SARS-CoV-2, SARS-CoV, and MERS-CoV Mpros. Crystal structures of Mpros from SARS-CoV-2, SARS-CoV, and MERS-CoV bound to the inhibitor YH-53 revealed a unique ligand-binding site, which provides new insights into the mechanism of inhibition of viral replication. A detailed analysis of these crystal structures defined the key molecular determinants required for inhibition and illustrate the binding mode of Mpros from other coronaviruses. In consideration of the important role of Mpro in developing antivirals against coronaviruses, insights derived from this study should add to the design of pan-coronaviral Mpro inhibitors that are safer and more effective.
Comment on Hietanen et al. Cytolytic Properties and Genome Analysis of Rigvir® Oncolytic Virotherapy Virus and Other Echovirus 7 Isolates. Viruses 2022, 14, 525
Authors: Alberts Pēteris
Published: September 2022

In a recent article published in Viruses by Hietanen et al. [...]
Antiviral Activity of Crude Polysaccharide Derived from Seaweed against IHNV and IPNV In Vitro
Authors: Ren Guangming , Xu Liming , Zhao Jingzhuang , Shao Yizhi , Lin Yujie , Li Linfang , Liu Qi , Lu Tongyan , Zhang Qiya
Published: September 2022

Both infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are the causative agents of acute and highly contagious diseases of juvenile salmonids, resulting in severe economic losses to these cold-water fish globally. There is an urgent need to explore antiviral agents against IHNV and IPNV due to the lack of commercially available vaccines and antiviral drugs. More importantly, the co-infection of IHNV and IPNV is prevalent in nature, which not only aggravates extensive damage to the salmonids but also poses challenges to its prevention and control. The antiviral effects of a crude polysaccharide derived from seaweed (CSP) on IHNV and IPNV were evaluated in this study separately. Furthermore, the underlying antiviral mechanisms of CSP to IHNV and IPNV were analyzed, respectively. The results showed that CSP possessed excellent safety and good ability to inhibit IHNV, IPNV, and their co-infection. CSP preferred to act at the early stage of viral infection. The antiviral mechanism of CSP on IHNV is possibly involved in preventing viral attachment and release, while in IPNV, it is involved in suppressing viral attachment, entry, and release. Taken together, the results of this study shed new light on developing novel agents against viral infection in salmonid fish.
Baculovirus-Free SARS-CoV-2 Virus-like Particle Production in Insect Cells for Rapid Neutralization Assessment
Authors: Jaron Marcel , Lehky Michael , Zarà Marta , Zaydowicz Chris Nicole , Lak Aidin , Ballmann Rico , Heine Philip Alexander , Wenzel Esther Veronika , Schneider Kai-Thomas , Bertoglio Federico , Kempter Susanne , Köster Reinhard Wolfgang , Barbieri Silvia Stella , van den Heuvel Joop , Hust Michael , Dübel Stefan , Schubert Maren
Published: September 2022

Virus-like particles (VLPs) resemble authentic virus while not containing any genomic information. Here, we present a fast and powerful method for the production of SARS-CoV-2 VLP in insect cells and the application of these VLPs to evaluate the inhibition capacity of monoclonal antibodies and sera of vaccinated donors. Our method avoids the baculovirus-based approaches commonly used in insect cells by employing direct plasmid transfection to co-express SARS-CoV-2 envelope, membrane, and spike protein that self-assemble into VLPs. After optimization of the expression plasmids and vector ratios, VLPs with an ~145 nm diameter and the typical “Corona” aura were obtained, as confirmed by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM). Fusion of the membrane protein to GFP allowed direct quantification of binding inhibition to angiotensin II-converting enzyme 2 (ACE2) on cells by therapeutic antibody candidates or sera from vaccinated individuals. Neither VLP purification nor fluorescent labeling by secondary antibodies are required to perform these flow cytometric assays.
Human Papillomavirus Vaccine Impact and Effectiveness in Six High-Risk Populations: A Systematic Literature Review
Authors: Goodman Elizabeth , Reuschenbach Miriam , Kaminski Allysen , Ronnebaum Sarah
Published: September 2022

Specific adult populations known to be at high risk for human papillomavirus (HPV)-related disease, such as men who have sex with men, are inconsistently included in national immunization programs. No compilation of the evidence on the real-world impact and effectiveness of HPV vaccines across these populations exists. This systematic literature review identifies and synthesizes the evidence of the real-world impact and effectiveness of the quadrivalent and nonavalent HPV vaccines in high-risk populations: women with prior/current HPV-related anogenital disease, men who have sex with men, immunocompromised/immunosuppressed individuals, female sex workers, transgender and non-binary individuals, and patients with recurrent respiratory papillomatosis (RRP). The outcomes included anogenital precancers/cancers, head and neck cancers, genital warts, and RRP recurrence. From the 2216 records identified, 30 studies (25 effectiveness and 5 impact studies) were included in this systematic literature review. The results, quantity, and quality of these studies were highly variable. The evidence for effectiveness was of high quality only in women with prior/current cervical disease and in individuals with RRP, the most frequently studied populations. No studies of transgender/non-binary individuals or female sex workers were identified. The real-world evidence supports HPV vaccination among women with prior cervical disease and individuals with RRP. Significant real-world data gaps remain in these high-risk populations.
Nanoclays: Promising Materials for Vaccinology
Authors: Govea-Alonso Dania O. , García-Soto Mariano J. , Betancourt-Mendiola Lourdes , Padilla-Ortega Erika , Rosales-Mendoza Sergio , González-Ortega Omar
Published: September 2022

Clay materials and nanoclays have gained recent popularity in the vaccinology field, with biocompatibility, simple functionalization, low toxicity, and low-cost as their main attributes. As elements of nanovaccines, halloysite nanotubes (natural), layered double hydroxides and hectorite (synthetic) are the nanoclays that have advanced into the vaccinology field. Until now, only physisorption has been used to modify the surface of nanoclays with antigens, adjuvants, and/or ligands to create nanovaccines. Protocols to covalently attach these molecules have not been developed with nanoclays, only procedures to develop adsorbents based on nanoclays that could be extended to develop nanovaccine conjugates. In this review, we describe the approaches evaluated on different nanovaccine candidates reported in articles, the immunological results obtained with them and the most advanced approaches in the preclinical field, while describing the nanomaterial itself. In addition, complex systems that use nanoclays were included and described. The safety of nanoclays as carriers is an important key fact to determine their true potential as nanovaccine candidates in humans. Here, we present the evaluations reported in this field. Finally, we point out the perspectives in the development of vaccine prototypes using nanoclays as antigen carriers.
Comparison of COVID-19 Vaccine Policies in Italy, India, and South Africa
Authors: Yang Manfei , Shi Leiyu , Chen Haiqian , Wang Xiaohan , Jiao Jun , Liu Meiheng , Yang Junyan , Sun Gang
Published: September 2022

(1) Purpose: This study aimed to analyze coronavirus disease 2019 (COVID-19) vaccine policies and their effectiveness in Italy, India, and South Africa to provide empirical experience for vaccination and COVID-19 pandemic control. (2) Methods: The study systematically summarized the COVID-19 vaccine policies in Italy, India, and South Africa through public information available on the official websites of the World Health Organization and the ministries of health in these three countries. Total vaccinations, COVID-19 vaccination rates, rates of fully vaccinated, rates of booster-vaccinated, and total confirmed cases were selected for cross-sectional comparison of COVID-19 vaccination in these three countries. Daily cases per million, daily deaths per million, and the effective reproduction rate were calculated to measure the effectiveness of COVID-19 vaccine policies implementation in each of these three countries. (3) Results: Italy, India, and South Africa differ in the start date of COVID-19 vaccination, vaccine types, vaccine appointments, and whether vaccinations are free. The COVID-19 vaccination rates in these three countries varied widely, with Italy having the highest and South Africa the lowest. COVID-19 vaccination has had a positive effect on reducing daily deaths and stabilizing the effective reproduction rate. The three countries had experienced more than one outbreak spike due to the spread of new mutated strains since the start of COVID-19 vaccination. (4) Conclusions: This study concluded that responding to the COVID-19 pandemic requires active promotion of basic and booster vaccinations to comprehensively build up the population immune barrier. Promoting equitable distribution of COVID-19 vaccine internationally and solidarity and cooperation among countries maximizes global common interests. By combining vaccination with non-pharmaceutical interventions, the pandemic can be prevented and controlled comprehensively and systematically in three aspects: detection of the source of infection, reduction of transmission routes, and protection of susceptible populations.
Reduced Prevalence of Measles Antibodies in a Cohort of Brazilian Children under 15 Years of Age
Authors: Estofolete Cassia Fernanda , de Andrade Gandolfi Flora , de Aguiar Milhim Bruno Henrique , da Silva Gislaine Celestino Dutra , Dourado Fernanda Simões , dos Santos Bárbara Ferreira , Vasilakis Nikos , Nogueira Maurício Lacerda
Published: September 2022

Measles is a highly contagious acute febrile disease responsible for sporadic but explosive outbreaks worldwide. Although it was considered eliminated from the Americas, the disease reemerged in 2017. This reemergence was linked to reduced immunization rates. Vaccination, early identification of cases, and blocking of the transmission chain are the most effective tools to combat measles, for which there is not a specific antiviral treatment. In this Brazilian prospective cross-sectional study, we investigated the prevalence of measles antibodies in children, a population vulnerable to significant morbidity and mortality from exposure to infection. Between December 2018 to November 2019, blood samples from 252 children were collected and assessed for the presence of measles-specific IgG antibodies and an overall prevalence of 75.8% was observed. We further stratified the cohort into age subgroups, none of which exhibited antibody presence levels above the herd immunity threshold of 94%. Moreover, the calculated number of secondary cases resulting from a case in any age group ranged from 3 to 4, demonstrating that even with partial vaccination coverage this disease is still concerning and highly transmissible. Despite global warnings about measles and previous efforts to combat the advance of the disease, data on antibody prevalence and vaccination coverage in several countries are still concerning and indicate that significant parts of the population could be affected. Seroprevalence studies like this one are important to highlight actual epidemiological conditions and emphasize the need for additional strategies to encourage immunization and avoid the potential impacts of measles, particularly in children who can be severely affected.
The Interplay of Oxidative Stress and ROS Scavenging: Antioxidants as a Therapeutic Potential in Sepsis
Authors: Kumar Sanni , Saxena Juhi , Srivastava Vijay Kumar , Kaushik Sanket , Singh Himadri , Abo-EL-Sooud Khaled , Abdel-Daim Mohamed M. , Jyoti Anupam , Saluja Rohit
Published: September 2022

Oxidative stress resulting from the disproportion of oxidants and antioxidants contributes to both physiological and pathological conditions in sepsis. To combat this, the antioxidant defense system comes into the picture, which contributes to limiting the amount of reactive oxygen species (ROS) leading to the reduction of oxidative stress. However, a strong relationship has been found between scavengers of ROS and antioxidants in preclinical in vitro and in vivo models. ROS is widely believed to cause human pathology most specifically in sepsis, where a small increase in ROS levels activates signaling pathways to initiate biological processes. An inclusive understanding of the effects of ROS scavenging in cellular antioxidant signaling is essentially lacking in sepsis. This review compiles the mechanisms of ROS scavenging as well as oxidative damage in sepsis, as well as antioxidants as a potent therapeutic. Direct interaction between ROS and cellular pathways greatly affects sepsis, but such interaction does not provide the explanation behind diverse biological outcomes. Animal models of sepsis and a number of clinical trials with septic patients exploring the efficiency of antioxidants in sepsis are reviewed. In line with this, both enzymatic and non-enzymatic antioxidants were effective, and results from recent studies are promising. The usage of these potent antioxidants in sepsis patients would greatly impact the field of medicine.
Insight into the Removal of Enoxacin in an Anaerobic Sulfur-Mediated Wastewater Treatment System: Performance, Kinetics and Mechanisms
Authors: Yan Yujian , Ou Yuyi , Yang Boyi , Jia Yanyan , Sun Lianpeng , Lu Hui
Published: September 2022

The removal of enoxacin (ENO), a broad-spectrum fluoroquinolone antibiotic, was firstly examined in a sulfate-reducing up-flow sludge bed (SRUSB) bioreactor over a long-term operation (366 days). Over 94% of the ENO was removed in the SRUSB bioreactor via adsorption and biodegradation at different initial ENO concentrations (i.e., 25–1000 μg/L). Based on the results of the batch tests, the sulfate-reducing sludge exhibited a high ENO adsorption capacity within a kd of 22.7–28.9 L/g-SS. The adsorption of ENO by the sulfate-reducing sludge was a spontaneous (ΔG° < 0 KJ/mol) and exothermic (ΔH° < 0 KJ/mol) process including physisorption and chemisorption (absolute value of ΔH° = 51.882 KJ/mol). Moreover, ENO was effectively biodegraded by the sulfate-reducing sludge within specific rates of 2.5–161.3 μg/g-SS/d. The ENO biodegradation process in the sulfate-reducing sludge system was most accurately described by the first-order kinetic model. Collectively, our findings provide insight into the applicability of a sulfate-reducing sludge system for ENO-contaminated wastewater treatment.
Hydraulic Transient Simulation of Pipeline-Open Channel Coupling Systems and Its Applications in Hydropower Stations
Authors: Zeng Wei , Wang Chao , Yang Jiandong
Published: September 2022

Hydraulic systems may involve both pipelines and open channels, which challenges the hydraulic transient analysis. In this paper, a method of characteristics (MOC)-finite volume method (FVM) coupling method has been developed with the pipeline modelled using the MOC and the open channel modelled using the FVM. The coupling boundaries between these two simulation regions are developed based on Riemann invariants. The simulated parameters can be transmitted from the MOC region to the FVM region and in the reverse direction through the coupling boundaries. Validation of the method is conducted on a simple tank-pipe system by comparing the simulated result using 3D computational fluid dynamics (CFD) analysis. The new method is then applied to a prototype hydropower station with a sand basin located between the upstream reservoir and the turbines. The sand basin is modelled as an open channel coupled with the pipes in the system. The transient processes are also simulated by modelling the sand basin as a surge tank. The comparison with the results by the MOC-FVM coupling method shows the new coupling method can provide more reliable and accurate results. This is because the flow velocity in the horizontal direction in the sand basin is considered in the coupling method but neglected when the sand basin is modelled as a surge tank in the MOC.
Integrated Risk Assessment of Waterlogging in Guangzhou Based on Runoff Modeling, AHP, GIS and Scenario Analysis
Authors: Xie Shuai , Liu Wan , Yuan Zhe , Zhang Hongyun , Lin Hang , Wang Yongqiang
Published: September 2022

Among the various natural disasters encountered by cities, rainstorm waterlogging has become a serious disaster, affecting the sustainable development of cities. Taking Guangzhou as the research object, based on disaster system theory and risk triangle theory, the evaluation framework “risk of hazard causing factors—sensitivity of disaster environment—vulnerability of hazard bearing body” was selected to construct the waterlogging risk assessment model of Guangzhou. The weighted comprehensive evaluation method (AHP) was used to determine the index weight, and the rainfall runoff inundation range under different rainstorm scenarios was deduced through a Soil Conservation Service (SCS) runoff generation model and GIS local equal volume passive inundation simulation. The results show that when the precipitation in 2 h is less than 100 mm, the inundation range increases by 3.4 km2 for every 10 mm increase in precipitation; When the precipitation in 2 h is greater than 100 mm, the inundation range will increase by 18 km2 for every 10 mm increase in precipitation. The total area of medium and high flood risk in Guangzhou is 441.3 km2, mainly concentrated in Yuexiu District, Liwan District, Haizhu District and Tianhe District.
Chromium Removal in the Presence of NOM during Fe(II) Reductive Precipitation for Drinking Water Treatment
Authors: Langer Margarethe , Jamal Mohammad Umair , Conklin Amanda , Ernst Mathias
Published: September 2022

Cr(VI) is toxic and carcinogenic, which fuels discussions on reducing existing standards for maximum Cr concentrations in drinking water. Fe(II) reductive precipitation is a common and economical method for achieving very low Cr(tot) concentrations (<5 µg/L). While Cr(VI) is reduced to Cr(III), Fe(II) is oxidized to Fe(III). The resulting Cr(III) and Fe(III) have low solubilities at neutral pH, precipitate as hydroxides, and can be removed by conventional media filtration. The presence of natural organic matter (NOM) in the raw water source can, depending on pH, concentration, type of NOM, and contact time, affect this process in various ways, from promoting Cr(VI) reduction, to re-reducing Fe(III), to forming stable complexes with Cr and Fe, thus, impairing chromium removal. The presented data showed that NOM, whether dominated by terrestrial humic acid, or of aquatic origin, could substantially impair chromium removal at neutral pH conditions. In particular, the ultimate removal of Cr(III) was affected. Soluble complexes and/or colloids of Cr(III), Fe(III) and NOM in the size range of 10 kDa–0.1 µm were formed, that could not be removed by conventional media filtration. Presence of iron sludge (>50 mg/L Fe(III)) mitigated the negative impacts of NOM on Cr(VI) reduction and Cr(III) removal. However, even 100 mg/L Fe(III), the highest applied sludge concentration, did not lead to a decrease in Fe(II) dosing requirements under the given conditions. A molar ratio higher than the given stochiometric ratio of [Fe(II)]:[Cr(VI)] = 3 was necessary for sufficient Cr(VI) removal.
A Framework for Water Security Data Gathering Strategies
Authors: Butte Giacomo , Solano-Correa Yady Tatiana , Peppa Maria Valasia , Ruíz-Ordóñez Diana Marcela , Maysels Rachael , Tuqan Nasser , Polaine Xanthe , Montoya-Pachongo Carolina , Walsh Claire , Curtis Thomas
Published: September 2022

At the international level, the term “water security” (WS) has gained increasing attention in recent decades. At the operational level, WS is assessed using tools that define the concept using a variety of dimensions and sub-dimensions, with qualitative and quantitative indicators and parameters. The breadth of tools and concepts is an obstacle to the operationalisation of the concept of water security (WS). Clearly, we need a range of diverse data to evaluate water security (WS). However, there are several barriers to designing an optimal Data Gathering Strategy (DGS). Such a strategy must strike a balance between a wide range of competing and overlapping data requirements and characteristics including: resources, information, and impact. The proposed framework aims at filling the existing gaps, not by providing a strict procedure, but instead acting as a “compass”: five interfaces between data and context are identified to orient practitioners towards an optimal DGS. The conceptual aim of the framework can be summarised as shifting the focus of the DGS from a “data-to-information approach” to a “data-to-action approach,” therefore stressing the importance of reaching key stakeholders with information. The specific aims of this paper are to: identify the key issues that should be addressed in designing a Data Gathering Strategy for Water Security (DGSxWS); communicate the key issues with a clear conceptual framework; and suggest approaches and activities that could help water practitioners in dealing with the issues identified.
Achieving Sustainable Development Goal 6 Electrochemical-Based Solution for Treating Groundwater Polluted by Fuel Station
Authors: da Silva Júlio César Oliveira , Solano Aline Maria Sales , Barbosa Segundo Inalmar D. , dos Santos Elisama Vieira , Martínez-Huitle Carlos A. , da Silva Djalma Ribeiro
Published: September 2022

Oil leakage occurs at fuel service stations due to improper storage, which pollutes soil and, subsequently, can reach the groundwater. Many compounds of petroleum-derived fuels pose hazards to aquatic systems, and so must be treated to guarantee clean and safe consumption, which is a right proposed by the United Nations in their Sustainable Development Goal 6 (SDG 6: Clean Water and Sanitation). In this study, contaminated groundwater with emerging pollutants by petroleum-derived fuel was electrochemically treated in constantly mixed 0.5 L samples using three different anodes: Ni/BDD, Ti/Pt, Ti/RuO2. Parameters were investigated according to chemical oxygen demand (COD), energy consumption analysis, by applying different electrodes, current densities (j), time, and the use of Na2SO4 as an electrolyte. Despite a similar COD decrease, better degradation was achieved after 240 min of electrochemical treatment at Ti/RuO2 system (almost 70%) by applying 30 mA cm−2, even without electrolyte. Furthermore, energy consumption was lower with the RuO2 anode, and greater when 0.5 M of Na2SO4 was added; while the order, when compared with the other electrocatalytic materials, was Ti/RuO2 > Ti/Pt > Ni/BDD. Thereafter, aiming to verify the viability of treatment at a large scale, a pilot flow plant with a capacity of 5 L was used, with a double-sided Ti/RuO2 as the anode, and two stainless steel cathodes. The optimal conditions for the effective treatment of the polluted water were a j of 30 mA cm−2, and 0.5 M of Na2SO4, resulting in 68% degradation after 300 min, with almost complete removal of BTEX compounds (benzene, toluene, ethyl-benzene, and xylene, which are found in emerging pollutants) from the water and other toxic compounds. These significant results proved that the technology used here could be an effective SDG 6 electrochemical-based solution for the treatment of groundwater, seeking to improve the quality of water, removing contaminants, and focusing on Brazilian environmental legislations and, consequently, converting pollutants into effluent that can be returned to the water cycle.
Effects of Diagenetic Alterations on Hydrocarbon Reservoirs and Water Aquifers
Authors: Mansurbeg Howri
Published: September 2022

Diagenesis includes all the biological, physical, chemical, biochemical, and physicochemical alterations that occur immediately after deposition and prior to low-grade metamorphism [...]