bluuebunny/crossref_metadata_2025 huggingface dataset:
Recurrent hemolytic uremic syndrome caused by DGKE gene mutation: a case report
Authors: Shin Baek Sup , Ahn Yo Han , Kang Hee Gyung
Published: June 2022

Atypical hemolytic uremic syndrome (aHUS) is a rare disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute kidney injury without any association with preceding diarrhea. Dysregulation of the complement system is the most common cause of aHUS, and monoclonal humanized anti-C5 antibodies are now recommended as the first-line treatment for aHUS. However, if the complement pathway is not the cause of aHUS, C5 inhibitors are ineffective. In this study, we report the second reported case of aHUS caused by DGKE mutations in Republic of Korea. The patient was an 11-month-old infant who presented with prodromal diarrhea similar to typical HUS, self-remitted with conservative management unlike complement-mediated aHUS but recurred with fever. While infantile aHUS often implies genetic dysregulation of the complement system, other rare genetic causes, such as DGKE mutation, need to be considered before deciding long-term treatment with C5 inhibitors.
Análise dos aspectos farmacológicos que envolvem o tratamento da depressão pós-parto: revisão sistemática
Authors: Santos Dejanilson Almeida dos , Silva Francisco Renan Reis da , Santos Natália Magalhães dos , Cardoso Rainara dos Santos
Published: June 2022

Embora a depressão possa começar em qualquer idade, a maioria das mulheres desenvolvem esses transtornos afetivos no pós-parto. A depressão pós-parto é um transtorno depressivo que ocorre quando há o aparecimento de episódios depressivos típicos durante o puerpério. Pertence à síndrome psiquiátrica puerperal, que é o mesmo que inquietação pós-parto e psicose pós-parto. O objetivo desse artigo é discorrer sobre os aspectos farmacológicos e atuação farmacêutica no manejo de pacientes com Depressão Pós-parto. Trata-se de uma a revisão sistemática, de natureza qualitativa, com objetivo exploratórios, usando-se como instrumentos de coleta de dados estudos publicados entre 2014 e 2022 nas bases de dados SCIELO, LILACS e BVS. Utilizou-se os descritores: “DPP”, “Depressão Pós-Parto” e “Tratamento de Depressão Pós-Parto”. Os estudos indicam que a puberdade, pré-menstruação, gravidez, puerpério e menopausa sempre suscitaram especulações sobre o peso dos fatores psicossociais e biológicos e suas possíveis interações na cadeia de causas das doenças psiquiátricas que ocorrem em uma conexão temporal, como nos processos gestacionais nas mulheres. A depressão pós-parto, em particular, levanta a questão de sua etiologia e terapia específicas. A depressão pós-parto, em particular, levanta a questão de sua etiologia e terapia específicas. Conclui-se que embora os antidepressivos sejam contraindicados em puérperas, a sua não administração traz efeitos mais sérios a saúde da mãe e de seu bebê. Com isso, demanda um acompanhamento farmacêutico como forma de mitigar os efeitos na paciente com DPP.
SISTEM INFORMASI PEMBAYARAN PARKIR BERBASIS WEB DENGAN MENGGUNAKAN MODEL WATERFALL
Authors: Bayu Aji Achmad Maezar , Rudianto Biktra
Published: June 2022

Seiring dengan bertambahnya jumlah penduduk dan meningkatnya kepemilikan kendaraan akan mempengaruhi pertumbuhan dan perkembangan kegiatan manusia didalamnya terutama pada kawasan yang memiliki prosentase yang tinggi atas kegiatan jasa, perdagangan, maupun komersil. Kendala muncul ketika sistem yang berjalan pada proses parkir masih belum terkomputerisasi dalam pengolahan data kendaraan masuk dan keluar serta perhitungan berapa lama waktu parkir masih menggunakan kalkulator untuk menentukan jumlah uang yang harus dibayar. Oleh karena itu diperlukan adanya sistem yang baik untuk mempermudah petugas parkir dalam melaksanakan tugasnya. Metode penelitian yang digunakan adalah metode waterfall yang didukung diagram UML dan bahasa pemrograman web. Hasil dari penelitian ini adalah sistem pembayaran parkir yang dapat terintegrasi dengan program sehingga lebih efektif dalam pengolahan data dan meminimalisir terjadinya kesalahan yang terjadi sebelumnya saat proses sistem belum terkomputerisasi.
PERANCANGAN ANIMASI INTERAKTIF PEMBELAJARAN PENGENALAN CIRI-CIRI KEASLIAN UANG RUPIAH
Authors: Hardani Sri , Wasiyanti Sri
Published: June 2022

Uang memiliki peranan yang sangat penting dalam kehidupan. Begitu pentingnya peran uang dalam kehidupan manusia, mendorong setiap orang melakukan berbagai hal untuk mendapatkannya, termasuk dengan cara yang tidak baik. Salah satu cara mendapatkan uang dengan cara yang tidak baik adalah dengan membuat uang palsu. Dalam kehidupan sehari-hari, masyarakat sulit membedakan uang asli dengan uang palsu jika hanya dilihat secara kasat mata. Banyak uang palsu yang beredar, memiliki tingkat kemiripan yang tinggi dengan uang asli. Bank Indonesia selaku lembaga yang behak mencetak dan mengatur peredaran uang di Indonesia terus berupaya meningkatkan kualitas keamanan pada uang kertas. Bank Indonesia menerapkan unsur pengamanan pada uang kertas Rupiah meliputi bahandan teknik cetak. Unsur pengamanan pada uang kertas Rupiah, sebagian besar bersifat terbuka sehingga dapat dikenali secara kasat mata. Selain itu, terdapat pula unsur pengamanan yang dapat dikenali dengan alat bantu sederhana seperti sunar ultraviolet dan kaca pembesar, serta unsur keamanan yang dapat dikenali dengan menggunakan mesin khusus. Tujuan dari penelitian ini adalah untuk menciptakan sebuah aplikasi yang dapat mengedukasi masyarakat mengenai ciri-ciri keaslian mata uang rupiah.
SISTEM INFORMASI PENJUALAN ALAT TULIS KANTOR BERBASIS WEB
Authors: Bayu Aji Achmad Maezar
Published: June 2022

Saat ini sering kali kita mendengar mengenai teknologi internet yang merupakan perkembangan terkini dari teknologi informasi. Dalam penjualan ATK masih terdapat kendala dalam pengolahan datanya yaitu masih belum efisien dan memerlukan waktu yang lebih lama karena belum diolah secara terkomputerisasi, dan juga dari segi pemasarannya masih menggunakan pamflet dan brosur dimana hal tersebut membutuhkan biaya yang tidak sedikit. Oleh sebab itu penulis membuat sistem yang lebih memudahkan dalam pengolahan data dan pemasarannya. Tujuan dari penelitian ini adalah dapat mempermudah penjualan alat tulis kantor secara efektif dan efisien dengan terhubung pada internet tanpa harus datang ke toko dan mencari dengan menanyakan ketersediaan alat tulis kantor yang di perlukan, serta penjual alat tulis kantor juga akan mudah untuk memasarkan produknya. Metode penelitian deskriptif kuantitatif adalah metode yang penulis gunakan karena dapat menganalisis dan memberikan gambar yang jelas dan terperinci melalui hasil wawancara, pengamatan data, mencari berbagai literatur terkait penelitian sehingga kesimpulan itu dapat ditarik dan untuk model yang digunakan menggunakan model air terjun yang didukung oleh UML dan pemrograman web. Hasil dari penelitian ini penulis membuat sistem penjualan ATK yang dapat digunakan secara online sehingga dapat mempermudah dalam proses penjualan dan pemasarannya.
PERANCANGAN SISTEM INFORMASI AKTIVA TETAP DENGAN MENGGUNAKAN FRAMEWORK SCRUM
Authors: Hardani Sri , Leliyanah Leliyanah
Published: June 2022

Aktiva tetap merupakan salah satu faktor pendukung dalam kegiatan operasional perusahaan dan bersifat tetap. Dalam perolehan aktiva tetap membutuhkan biaya yang besar dan diharapkan dapat dipergunakan dalam jangka waktu yang lama. Sebagai modal yang digunakan dalam kegiatan operasional perusahaan, maka nilai aktiva tetap akan mengalami penurunan. Dengan menurunnya nilai aktiva tetep seiring penggunaannya dalam operasional perusahaan, maka perlu dihitung biaya penyusutan aktiva tetap.  Sebuah perusahaan yang merupakan perusahaan manufaktur yang dalam menjalankan usahanya membutuhkan berbagai macam aktiva tetap, seperti mesin-mesin, kendaraan, peralatan kantor, dan lain-lain. Banyaknya jumlah aktiva tetap dan proses pencatatan yang masih menggunakan Microsoft Excel tentunya memberikan kesulitan tersendiri bagi petugas yang melakukan pencatatan dan menginventarisir aktiva tetap. Kesalahan dalam menghitung biaya penyusutan, kesalahan dalam menginput nominal, kesalahan dalam menghitung nilai buku aktiva tetap, lupa menginput biaya penyusutan di tanggal yang seharusnya, merupakan beberapa contoh permasalahan yang sering terjadi. Dengan adanya sistem informasi aktiva tetap, akan dapat mengurangi beban kerja paetugas, sekaligus dapat memberikan informasi yang lebih akurat mengenai data aktiva tetap. Pengembangan aplikasi dengan metode scrum diharapkan dapat menghasilkan aplikasi yang lebih sesuai dengan kebutuhan pengguna. Karena dengan metode scrum, segala bentuk perubahan kebutuhan pengguna dapat dengan mudah disesuaikan tanpa harus menunggu aplikasi selesai secara keseluruhan.
RANCANG BANGUN APLIKASI PENGADAAN BARANG DINAS KEBUDAYAAN DAN PARIWISATA KABUPATEN BOGOR BERBASIS WEBSITE
Authors: Darono Hanafi Eko
Published: June 2022

The goods procurement system at the Bogor Regency Culture and Tourism Office is still carried out conventionally so that it is still difficult to find data on goods that have been purchased. The system cannot find out the existence of office goods because the number of goods are not in accordance with procurement, especially regarding to the preparation of reports, namely procurement recapitulation report is not effective. Because of these conditions, it is very necessary to have a procurement application as a work aid in data processing and procurement reports. This research IS conducted to provide alternative solutions by referring to the need of business and employees relationship activities. The expected result is that the application can be used for procurement of goods at the Department of Culture and Tourism based on the website and it can be applied directly to the working field in order to facilitate the effectiveness of performance and minimize the level of errors.
PENINGKATAN KUALITAS CITRA BAWAH AIR BERBASIS ALGORITMA FUSION DENGAN KESEIMBANGAN WARNA, OPTIMALISASI KONTRAS, DAN PEREGANGAN HISTOGRAM
Authors: Suharyanto Suharyanto , Frieyadie Frieyadie , Kuryanti Sandra Jamu
Published: June 2022

Para peneliti saat ini memberikan perhatian yang cukup besar terhadap obyek citra bawah air, kebutuhan akan aplikasi pengamatan citra bawah air memiliki peran yang sangat penting dalam mengidentifikasi objek, pemantauan kehidupan spesies, deteksi kebocoran pipa minyak atau gas, pemantauan polusi, dan sebagainya. Degradasi citra bawah air merupakan fenomena atmosfer yang merupakan hasil dari hamburan dan penyerapan cahaya. Kami menggunakan satu gambar terdistorsi untuk mendapatkan kontras yang ditingkatkan dan versi koreksi warna dari gambar aslinya. Selanjutnya menghilangkan distorsi dan meningkatkan visibilitas objek dalam gambar dengan menerapkan peta bobot, hal ini dapat dicapai dengan menerapkan penyeimbangan warna putih, kemudian penajaman menggunakan gaussian filtering dilakukan untuk, meningkatkan tampilan visual. pada setiap input yang di proses. Langkah terakhir dilakukan menaikan nilai kontras dengan koreksi peregangan kontras yang dibatasi utuk meningkatkan warna keabuan dan uuntuk menhjilangkan efek noise yang berlebihan pada latar belakang obyek gambar. Hasil penelitian evaluasi menggunakan fitur Root Mean Squared Error (RMSE), dan Peak Signal-to-Noise Ratio (PSNR) dan kami bandingkan dengan metode CLAHE. Hasil evaluasi menunjukkan  peningkatan PNSR yang signifikan kualitas citra yg di perbaiki menggunakan algoritma kombinasi dibanding dengan menggunakan metode CLAHE sebagaimana yang kami paparkan dalam bagian hasil penelitian.
Physical and environmental drivers of oceanic manta ray Mobula birostris sightings at an aggregation site in Bahía de Banderas, Mexico
Authors: Fonseca-Ponce IA , Zavala-Jiménez AA , Aburto-Oropeza O , Maldonado-Gasca A , Galván-Magaña F , González-Armas R , Stewart JD
Published: June 2022

Oceanic manta raysMobula birostrisare distributed in tropical and subtropical oceans with aggregation sites typically at oceanic islands and seamounts. However, in some regions the species is found in coastal habitats close to highly productive areas. Recently, an important aggregation site for oceanic manta rays was identified along the southern coast of Bahía de Banderas, Mexico. We conducted weekly monitoring trips to the aggregation hotspots over a period of 4½ yr, recording manta sightings and collecting zooplankton samples, to investigate relationships between sightings and prey availability. We evaluated relationships between manta sightings and physical, biological, and environmental variables, finding a seasonal signal of manta occurrence with a peak in sightings around April, although there were substantial deviations from this trend in some years. Our results show effects of the El Niño Southern Oscillation on sighting rates, with more mantas during La Niña phases. We found significant relationships between manta sightings and sea surface temperature, moon phase, and tidal range. Manta sightings were negatively related to fish egg densities, positively related to copepod and cladoceran densities, and showed no relationship to euphausiid density. Mantas may be feeding on mesopelagic prey in the submarine canyon adjacent to the southern coast and basking in shallow waters through the day during thermal recovery periods. Identifying the seasonal patterns of occurrence and the environmental drivers of sightings will support the development of strategies to mitigate anthropogenic threats, which are common in this coastal population.
THE ROLE OF E-LEARNING IN OPTIMIZING STUDENT’S MATHEMATICS LITERATION
Authors: Amina  , Gusniawati  , Sanjaya Herlan
Published: June 2022

Changing the old pattern to a new pattern is one form of position in optimizing learning activities. Changes in this pattern, namely from teacher-centered patterns to new student-centered patterns through the network era have a broad impact. The development of internet technology has a very big influence on learning. E-learning media makes it easier to complete activities or activities through accessing information and subject matter whenever and wherever. The method used in this study, namely using library searches and journal reviews. The use of E-learning is very important in providing students to compete in this globalization era. The role of E-learning media is very helpful for teachers in maximizing the seven main components of mathematical literacy, namely communication, mathematics, representation, thinking, designing strategies, using formal symbols and technical operations. and using mathematical tools.
VALIDITY OF STUDENT WORKSHEET DEVELOPMENT BASE ON PBM WITH A SCIENTIFIC APPROACH IN THE MATERIAL SPECIAL LINES AND CIRCLES ON TRIANGLES
Authors: Putri Finola Marta
Published: June 2022

This research is in development research, aiming to develop student worksheets material Special Lines and Circles in Triangles based on Problem Based Learning with a scientific approach. This Student Work Sheet can be used as a learning resource by students in the Mathematics subject at School I in the Mathematics Education Department, Faculty of Tarbiyah and Teacher Training (FITK) UIN Syarif Hidayatullah Jakarta (UIN Jakarta). This study uses a 4-D development model consisting of four steps: defining, designing, developing, and disseminating. This research is limited to the development stage. The data collected is in the form of data on the validity of the Student Work Sheet. Validity data collected by circulating a validation questionnaire to two validators. The validity data is processed quantitative and qualitative. The data obtained based on the results of the analysis are(1) the quality of the material, (2) aspects of language and (3) overall elements of the presentation of the Student Keja Sheet material developed very valid criteria. Conclusion of The Student Work Sheet material Unique Lines and Circles on Triangles based on Problem-Based Learning with a scientific approach developed is suitable for use as a learning resource for students in the School Mathematics course I.
ANALYSIS OF STUDENT ERRORS IN SOLVING THE SYSTEMS OF INEQUALITIES WITH TWO VARIABLES PROBLEMS BASED ON NEWMAN
Authors: Erni Resi
Published: June 2022

Mathematics can help us to think logically, critically, and creatively. When taking mathematics lessons, there are still many students who make mistakes in solving math problems. So, it is necessary to do an analysis regarding this error. The stages of error analysis that are appropriate and that can be carried out in the form of Two Variable Inequality System questions are the stages of error analysis according to Newman (NEA). Newman suggests that if students want to solve math problems in the form of story problems, they through five steps, namely asking students to: (1) read the questions (reading), (2) understand the problem (comprehension), (3) transformation, (4) process skills, and (5) writing final answer (encoding). This research was conducted at SMA Negeri 1 Hulu Kuantan in the odd semester of the 2020/2021 school year. The sample of this study was 25 students of class XII MIPA 1. This research was conducted on Thursday, November 26, 2020. The research method is descriptive research. Based on the analysis conducted, it was found that the percentage of students' errors was 21.6% of errors in question number 1, the error in number 2 was 35.7%, the error in number 3 was 48%, the error in number 4 was 26.7%. From the data processing, it was found that the most student errors occurred in question number 3. The percentage of reading error was 36%, comprehension error was 72%, transformation error was 56%, process skill error was 64%, and encording error was 52%.
USING OF ONLINE MATHEMATICS LEARNING MEDIA IN CLASS VIII SMP NEGERI 1 PASIR PENYU
Authors: Nisa Khairun , Handayani Desti
Published: June 2022

The COVID-19 pandemic has significantly impacted various sectors, such as health, social, political, economic, and education. Educators must ensure that teaching and learning activities continue, even though students are home. The Central and Regional Governments took the Large-Scale Social Restriction Policy (PSBB) to contain the spread of the Corona-19 virus, which has disrupted the face-to-face learning process. The solution, educators design learning media as innovations by utilizing bold media (online). This is in accordance with the Minister of Education and Culture of the Republic of Indonesia regarding Circular Letter Number 4 of 2020 concerning the Implementation of Education Policies in the Emergency Period for the Spread of Corona Virus Disease (COVID-19). Educators can do learning together simultaneously using groups on social media such as WhatsApp (WA), telegram, Instagram, zoom application, google meet, learning videos via youtube links, and other media as learning media. The method used in writing this article is a qualitative method that describes something based on data and numbers narrated in concluding sentences. Besides that, the writing of this article is library research.
DEVELOPMENT OF MATHEMATICAL LEARNING DEVICES BASED ON PROBLEM BASED LEARNING MODEL ON THREE DIMENSIONAL MATERIAL TO FACILITATE HIGHER-ORDER THINKING SKILLS FOR STUDENTS IN CLASS XII SMA/MA
Authors: Samsi Nur , Saragih Sehatta , Anggraini Rini Dian
Published: June 2022

This research produces a product in the form of a mathematics learning device using a Problem Based Learning model on Three Dimensional material to facilitate students' Higher Order Thinking Ability. Validity and practicality is the instrument of the research. 4-D is a model of development. The validity instrument is in the form of a validation sheet to assess the validity of the learning tools, namely the syllabus, RPP, and LAS. The practicum instrument is a student response questionnaire to assess the usefulness and ease of use of LAS. Three validators will validate it and revise it according to the validator's suggestions. Then a limited group trial was conducted with six students. The results of syllabus validation 3.79, RPP 3.73, and LAS 3.58 are very valid. While the result of LAS is 85.15%, it is said to be a very practical category. Based on these, it was concluded that this study produced a product in the form of a mathematical learning device based on the Problem Based Learning model on Three Dimensional material that was valid for syllabus, RPP, and LAS and practical for LAS.
Hutcheson and his Critics and Opponents on the Moral Sense
Authors: Boeker Ruth
Published: June 2022

This paper takes a new look at Francis Hutcheson's moral sense theory and examines it in light of the views of his rationalist critics and opponents who claim that there has to be an antecedent moral standard prior to any sense or affections. I examine how Gilbert Burnet, Samuel Clarke, and Catharine Trotter Cockburn each argue for the priority of reason over a moral sense and how Hutcheson responds or could respond to their views. Furthermore, I consider the proposal that rather than regarding Hutcheson's moral sense theory as fundamentally opposed to moral rationalism, Hutcheson and Clarke endorse a shared moral metaphysics, as argued by Patricia Sheridan. Although I consider this proposal as too broad and believe it overlooks relevant metaphysical differences between Clarke and Hutcheson, I argue that the dispute between Hutcheson and his critics and opponents will not be settled without taking their underlying moral metaphysics into consideration.
TOURISM BROKERS' PERSPECTIVE ON TURKEY'S IMAGE: MARKETING COMMUNICATIONS AND SUBJECTIVE NORMS AS MEDIATORS
Authors: Najjarzadeh Mohammad , Amirzade Nazanin , Asadi Zarch Mohammade Reza
Published: June 2022

There is growing investment in the tourism industry, especially in the countries with potential attractions. All tourism destinations attempt to compete with other opponents and be distinctive. They also try to provide more particular experiences for visitors. The present study aims to investigate the effectiveness of the tourism brokers’ perspective on Turkey's image as a tourism destination through the mediating role of marketing communications and subjective norms. This study is an applied research based on descriptive correlational studies. The research population includes all the tourism brokers in Iran (travel agencies, tour operators, and travel agents) who provide outgoing Turkey tours. The samples (including 250 tourism brokers) are selected using a convenience sampling method and data were collected by using a questionnaire. To analyze data, Structural Equation Modeling (SEM) is applied using the Smart PLS 3 software. Results show that cognitive and affective images have a significant direct effect on tourism destination image while they have a significant indirect effect on destination image through the mediating role of marketing communications and subjective norms. Marketing Communications and Subjective Norms have a significant direct effect on destination image. Previously, there were not adequate information on and attention to the factors affecting tourism brokers in promoting Turkey as a cross-border destination, so this study is conducted to present new ideas for marketers and tourism brokers.
THE ROLES OF TECHNOLOGY ACCEPTANCE, USER CREDIBILITY AND COVID-19 PERCEIVED HEALTH RISK IN TOURIST’S SOCIAL MEDIA USER-GENERATED-CONTENT USAGE INTENTION
Authors: Hanafiah Mohd Hafiz , Md Zain Adilah , Asyraff Muhammad Aliff , Makhasi Ghifari Yuristiadhi Masyhari
Published: June 2022

This study examines the direct effects of technology acceptance (perceived usefulness and ease of use), trustworthiness, expertise, and the moderating effect of COVID-19 perceived health risk on tourists’ social media User-Generated-Content (UGC) usage intention. The study respondent was 233 Malaysian tourists who travelled domestically for leisure during the COVID-19 pandemic. The study hypotheses were examined using multiple regression analysis. The hypotheses testing indicates that perceived usefulness, ease of use, trustworthiness, and expertise significantly affect future usage of social media UGC. In addition, the findings confirm that perceived health risk does not affect the user trustworthiness and future usage of social media UGC. The findings assist tourism stakeholders in understanding tourist behaviour towards social media UGC, especially during the COVID-19 pandemic. The tourism business and policymakers should continuously improve and monitor their UGC platform, especially since UGC was deemed one of the vital information dissemination channels during the COVID-19 pandemic.
NON-PROFIT ORGANIZATIONS AND TOURISM: CAN TOURISM DRIVE NON-PROFIT SECTOR DEVELOPMENT?
Authors: Dadic Fruk Lorena , Durkin Badurina Jelena , Maškarin Ribaric Helga
Published: June 2022

Non-profit sector development is the basis of any modern society. Various approaches, which take into consideration a broad spectrum of factors, are used in measuring the level of non-profit sector development. Previous studies do not identify tourism as a potential factor. It can, however, be assumed that local authorities in better-developed tourism regions are able to allocate more funds to non-profit activities, thus affecting the size of the non-profit sector. It would also be necessary to investigate whether nonprofit organizations (NPOs) recognise the potential that tourism has to provide them with opportunities to generate their own income. To determine the relationship between tourism and the non-profit sector, cluster analysis was conducted for 20 Croatian counties. The results of the study show that the level of tourism development determines the development of the non-profit sector. Namely, results confirm that there are more NPOs in regions with more-developed tourism and there are also more NPOs registered in fields of activity that can have a direct or indirect effect on tourism. On the other hand, the study’s findings indicate that regions with developed tourism tend to allocate more funds to NPO activity, which in turn boosts the level of non-profit sector development in those regions. Although this research focused on only one country and deal with certain challenges in administrative data on registered non-profit organisations, it is promising start for development of new lines of research oriented on connecting non-profit and tourism sector and examining their inter-dependence.
MODELLING TOURISM DEMAND USING GOOGLE ANALYTICS: A CASE STUDY OF PORTUGAL’S ALENTEJO REGION
Authors: Dinis Maria Gorete Ferreira , Eusébio Maria Celeste , Breda Zélia , Madaleno Ana
Published: June 2022

The development of information and communication technologies, specifically the Internet, has changed the way tourists plan their trips, being one of the most important information sources for tourism decision-making. However, a limited number of studies has been developed to analyse the causal relationships between the web interaction and tourism demand. Therefore, this paper intends to shed light on the usefulness of big data analytics to understand the tourism demand of a destination. More specifically,it aims to examine the causal relationship between website’s visitor interactions and the tourism demand of a destination and verify whether there are differences in this relationship according to the visitors' country of origin. In order to achieve the research objectives, the Alentejo region in Portugal was selected as a case study. Monthly data for the period between 2007 and 2017 was used to examine the long-run causalrelationship between the sessions of the users to the official website of the Destination Management Organization of Alentejo (measured through Google Analytics) and tourism demand of this region (measured trough the number of guests in tourism accommodation establishments). To analyse if there are differences in this relationship according to the country of origin of visitors, the most important tourism markets for this destination were selected. Cointegration (Johansen´s maximum-likelihood method), Granger causality test, Vector Autoregression Model, and Vector Error Correction Model were used to examine the relationship. The results reveal a causal relationship between Internet search and the tourism demand. However, this relationship varies among the tourism market analysed.
Monetary integration in South America: Elección of candidates through unsupervised machine learning
Authors: Padilla León , Marín Eduardo
Published: June 2022

Applying Unsupervised Machine Learning techniques to a set of nominal variables (based on the optimum currency area [OCA] theory and the Maastricht Treaty criteria) and industrial indicators (based on similar production patterns), this paper aims to identify potential candidates for a monetary integration in South America (SA). The main conclusion is that, according to the clustering of the nominal and industrial indicators, the countries in best position for a hypothetical monetary integration in SA are Chile, Colombia, and Perú (and Ecuador to a lesser extent); countries that are generally members of the same cluster. This group of economies, which belong to the Pacific Alliance, are in a better position to meet various criteria for regional monetary integration, such as nominal convergence and similar production patterns.
Bienestar e integración en América Latina: el papel de la IED
Authors: Hernández Mota José Luis , Marín Medrano Iván Abdel
Published: June 2022

Se analiza la relación entre la IED, la integración comercial, la actividad económica y el bienestar individual de economías representativas de América Latina: Brasil, Chile, Colombia y México. Se utiliza un modelo empírico con datos panel bajo la hipótesis de que la IED puede generar incrementos productivos y el consiguiente beneficio de la integración a nivel del bienestar individual. Aunque se aporta evidencia para consolidar y fortalecer los procesos de integración llevados a cabo, dada la heterogeneidad económica, los resultados obtenidos también permiten mostrar la importancia de las medidas de política económica en una economía receptora de IED.
Adriamycin-Induced Nephropathy is Robust in N and Modest in J Substrain of C57BL/6
Authors: Bryant Claire , Cianciolo Rachel , Govindarajan Rajgopal , Agrawal Shipra
Published: June 2022

Adriamycin (ADR)-induced nephropathy remains the leading model to study human primary focal segmental glomerulosclerosis (FSGS), a common pathway for podocyte damage and glomerular loss of function that leads to chronic kidney disease. However, the use of this model for reverse genetics is limited by historical categorization of C57BL/6 mice as an ADR-resistant strain, which is also the most common genetically modified strain. Additionally, conflicting reports exist utilizing C57BL/6 for ADR-nephrosis due to lack of understanding of substrain differences (J/N) and variability in ADR dosage, timing, and frequency to induce damage. We have undertaken a systematic approach to elucidate the specifics of ADR-nephrosis in C57BL/6 N and J substrains. We induced nephropathy with 2 doses of ADR, and measured albuminuria for 6 weeks and performed histological evaluations. Our findings revealed induction of robust and modest proteinuria in N and J substrains, respectively. The serum creatinine levels were elevated in N, but not J substrain. Both the substrains showed reduction in body weight with N greater than J, although mortality remained at 0% in both substrains. Histological analysis showed worse renal lesions in the N than the J substrain. Podocyte markers synaptopodin, nephrin, podocin, and WT1 were reduced to a greater extent in the N than the J substrain. In summary, we provide the nephrology community with a reproducible mouse model for FSGS, in a strain otherwise assumed to be ADR-resistant and highlight the differences between J and N substrains. This enables future studies, especially concerning genetically manipulated animal models in C57BL/6.
Characterization of the Achromobacter xylosoxidans Type VI Secretion System and Its Implication in Cystic Fibrosis
Authors: Le Goff Mélanie , Vastel Manon , Lebrun Régine , Mansuelle Pascal , Diarra Ava , Grandjean Teddy , Triponney Pauline , Imbert Geneviève , Gosset Philippe , Dessein Rodrigue , Garnier Fabien , Durand Eric
Published: June 2022

Bacteria of the genus Achromobacter are environmental germs, with an unknown reservoir. It can become opportunistic pathogens in immunocompromised patients, causing bacteremia, meningitis, pneumonia, or peritonitis. In recent years, Achromobacter xylosoxidans has emerged with increasing incidence in patients with cystic fibrosis (CF). Recent studies showed that A. xylosoxidans is involved in the degradation of the respiratory function of patients with CF. The respiratory ecosystem of patients with CF is colonized by bacterial species that constantly fight for space and access to nutrients. The type VI secretion system (T6SS) empowers this constant bacterial antagonism, and it is used as a virulence factor in several pathogenic bacteria. This study aimed to investigate the prevalence of the T6SS genes in A. xylosoxidans isolated in patients with CF. We also evaluated clinical and molecular characteristics of T6SS-positive A. xylosoxidans strains. We showed that A. xylosoxidans possesses a T6SS gene cluster and that some environmental and clinical isolates assemble a functional T6SS nanomachine. A. xylosoxidans T6SS is used to target competing bacteria, including other CF-specific pathogens. Finally, we demonstrated the importance of the T6SS in the internalization of A. xylosoxidans in lung epithelial cells and that the T6SS protein Hcp is detected in the sputum of patients with CF. Altogether, these results suggest for the first time a role of T6SS in CF-lung colonization by A. xylosoxidans and opens promising perspective to target this virulence determinant as innovative theranostic options for CF management.
Expanding the Malaria Antibody Toolkit: Development and Characterisation of Plasmodium falciparum RH5, CyRPA, and CSP Recombinant Human Monoclonal Antibodies
Authors: Nacer Adéla , Kivi Gaily , Pert Raini , Juronen Erkki , Holenya Pavlo , Aliprandini Eduardo , Amino Rogerio , Silvie Olivier , Quinkert Doris , Le Duff Yann , Hurley Matthew , Reimer Ulf , Tover Andres , Draper Simon J. , Gilbert Sarah , Ho Mei Mei , Bowyer Paul W.
Published: June 2022

Malaria, an infection caused by apicomplexan parasites of the genusPlasmodium, continues to exact a significant toll on public health with over 200 million cases world-wide, and annual deaths in excess of 600,000. Considerable progress has been made to reduce malaria burden in endemic countries in the last two decades. However, parasite and mosquito resistance to frontline chemotherapies and insecticides, respectively, highlights the continuing need for the development of safe and effective vaccines. Here we describe the development of recombinant human antibodies to three target proteins fromPlasmodium falciparum: reticulocyte binding protein homologue 5 (PfRH5), cysteine-rich protective antigen(PfCyRPA), and circumsporozoite protein (PfCSP). All three proteins are key targets in the development of vaccines for blood-stage or pre-erythrocytic stage infections. We have developed potent anti-PfRH5,PfCyRPA andPfCSP monoclonal antibodies that will prove useful tools for the standardisation of assays in preclinical research and the assessment of these antigens in clinical trials. We have generated some very potent anti-PfRH5 and anti-PfCyRPA antibodies with some clones >200 times more potent than the polyclonal anti-AMA-1 antibodies used for the evaluation of blood stage antigens. While the monoclonal and polyclonal antibodies are not directly comparable, the data provide evidence that these new antibodies are very good at blocking invasion. These antibodies will therefore provide a valuable resource and have potential as biological standards to help harmonise pre-clinical malaria research.
Using Selected Members of a Large Ensemble to Improve Prediction of Surface Air Temperature Anomalies Over Japan in the Winter Months From Mid-Autumn
Authors: Ratnam J. V. , Doi Takeshi , Richter Ingo , Oettli Pascal , Nonaka Masami , Behera Swadhin K.
Published: June 2022

A large ensemble of 120 predictions of the Scale Interaction Experiment-Frontier Research Center for Global Change Version 2 (SINTEX-F2) coupled general circulation model were evaluated for their skill in predicting the surface air temperature (SAT) anomalies over Japan in the winter months December, January, and February. The predictions were initialized using November initial conditions. The members with skill scores based on anomaly correlation coefficient (ACC) were selected and an average of the selected predictions (SEM) was generated. Comparison of SAT anomaly predictions by the average of all the 120 members (ENS) to the SEM predictions shows SEM to outperform the ENS predictions in all the three winter months with higher ACC skill score, higher hit rate and low false alarm rate over the regions covering central Japan in December and January and over the northern region of Hokkaido in February. The improvement in the skill scores in the SEM is found to be due to improved representation of 200 hPa geopotential height anomalies in SEM compared to ENS predictions. The results indicate SEM to be useful for improving skill in predicting SAT anomalies over parts of Japan in the winter months.
Communication Technologies and Aid Practices: Superbergamo, Group Chats, and the COVID-19 Pandemic
Authors: Parolin Laura Lucia , Pellegrinelli Carmen
Published: June 2022

This article examines “Superbergamo”, a collective which emerged in response to the needs of vulnerable citizens during the first wave of the COVID-19 pandemic in Bergamo (Italy). The analysis reveals the central role of social media and group chat systems in facilitating the spontaneous creation of the group, and the subsequent development of coordinated voluntary emergency activities that brought aid to thousands of local people. Inspired by Actor-Network theory, the analysis traces of the group's emergence, showing how human and non-human actors—including social media and group chat apps—played a crucial role in shaping the aid practice. In so doing, we contribute to the literature on emergency responses from the public and social media. More significantly, we show how the critical contribution of technology to the development and sustenance of aid practices can be mapped, by providing evidence of how groups, practices and sociomaterial networks are necessarily entangled.
Risk and Protective Factors for Sudden Cardiac Death: An Umbrella Review of Meta-Analyses
Authors: Tsartsalis Dimitrios , Korela Dafni , Karlsson Lars O. , Foukarakis Emmanouil , Svensson Anneli , Anastasakis Aris , Venetsanos Dimitrios , Aggeli Constantina , Tsioufis Costas , Braunschweig Frieder , Dragioti Elena , Charitakis Emmanouil
Published: June 2022

Background

Sudden cardiac death (SCD) is a global public health issue, accounting for 10–20% of deaths in industrialized countries. Identification of modifiable risk factors may reduce SCD incidence.

Methods

This umbrella review systematically evaluates published meta-analyses of observational and randomized controlled trials (RCT) for the association of modifiable risk and protective factors of SCD.

Results

Fifty-five meta-analyses were included in the final analysis, of which 31 analyzed observational studies and 24 analyzed RCTs. Five associations of meta-analyses of observational studies presented convincing evidence, including three risk factors [diabetes mellitus (DM), smoking, and early repolarization pattern (ERP)] and two protective factors [implanted cardiac defibrillator (ICD) and physical activity]. Meta-analyses of RCTs identified five protective factors with a high level of evidence: ICDs, mineralocorticoid receptor antagonist (MRA), beta-blockers, and sodium-glucose cotransporter-2 (SGLT-2) inhibitors in patients with HF. On the contrary, other established, significant protective agents [i.e., amiodarone and statins along with angiotensin-converting enzyme (ACE) inhibitors in heart failure (HF)], did not show credibility. Likewise, risk factors as left ventricular ejection fraction in HF, and left ventricular hypertrophy, non-sustain ventricular tachycardia, history of syncope or aborted SCD in pediatric patients with hypertrophic cardiomyopathy, presented weak or no evidence.

Conclusions

Lifestyle risk factors (physical activity, smoking), comorbidities like DM, and electrocardiographic features like ERP constitute modifiable risk factors of SCD. Alternatively, the use of MRA, beta-blockers, SGLT-2 inhibitors, and ICD in patients with HF are credible protective factors. Further investigation targeted in specific populations will be important for reducing the burden of SCD.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020216363, PROSPERO CRD42020216363.
The Clinical Trial Outcomes of Med-Zenith PT-Valve in the Treatment of Patients With Severe Pulmonary Regurgitation
Authors: Shang Xiaoke , Dong Nianguo , Zhang Changdong , Wang Yanggan
Published: June 2022

Objective

Nearly 2/3 of patients with dilated right ventricular outflow tract (RVOT) were excluded from pulmonary valves transplantation due to the lack of size-matched valves. Here, we explored the safety and efficacy of the Med-Zenith PT-Valve for the treatment of patients with severe pulmonary regurgitation.

Methods

22 Patients with severe PR (grade 3+,4+) were enrolled based on the anatomical features of native RVOT and the valve design. The immediate, 3-months and 1-year post-procedural follow-up data were analyzed.

Results

The baseline mean systolic diameters in the distal main pulmonary artery (MPA), MPA sinus junction, MPA sinus, pulmonary annulus, RVOT aneurysm and muscular outlet measured with computed tomography were 33.6 ± 6.1, 34.0 ± 5.8, 37.9 ± 6.0, 32.4 ± 7.3, 41.9 ± 9.3, and 34.4 ± 8.0 mm, respectively. The PT-Valve landing zone was set within these levels. Successful valve implantations were achieved in all patients without noticeable device malposition, coronary artery compression, pulmonary branch obstruction or paravalvular leak during follow-ups. Post-procedural pulmonary artery diastolic pressure increased from 5.8 ± 3.1 to 11.3 ± 2.5 mmHg. In the 3-month and 1-year follow-up, the right ventricular end diastolic volume index reduced from the baseline 181.6 ± 29.0 to 143.7 ± 29.7 ml/m2 and 123.4 ± 31.2 ml/m2, and the trans-pulmonary valve gradient decreased from 25.6 ± 22.2 to 10.64 ± 3.54 mmHg and 11.16 ± 3.0 mmHg, respectively. The 6-min walk distance increased from 416.6 ± 97.9 to 455.9 ± 64.6 m and 467.8 ± 61.2 m, respectively.

Conclusion

This clinical trial revealed favorable outcomes for the safety, efficacy and feasibility of the Med-Zenith PT-Valve in the treatment of severe PR with significantly enlarged RVOT.
Admission Lysophosphatidic Acid Is Related to Impaired Kidney Function in Acute Aortic Dissection: 2-Year Retrospective Follow-Up Study
Authors: Pan Xiaogao , Yang Guifang , Ding Ning , Peng Wen , Guo Tuo , Zeng Mengping , Chai Xiangping
Published: June 2022

Background

Delayed treatment of acute aortic dissection (AAD)-related acute kidney injury (AKI) significantly increases the burden of chronic kidney disease (CKD) and mortality. Lysophosphatidic acid (LPA) is a shared mediator of kidney disease and AAD. Here, we evaluated the relationship between LPA and kidney injury in AAD patients.

Methods

We measured the plasma concentration of LPA in a cohort of 80 patients with AAD. Least Absolute Shrinkage and Selection Operator (LASSO) regression and Logistic regression were used to evaluate the effect and interaction of LPA on AKI. Additive generalized model and penalized spline method were used to describe the non-linear association. Multivariable analyses with the Cox proportional-hazards model were used for subgroup analysis and interaction in LPA and subsequent CKD.

Results

The participant’s average age was 54.27 ± 11.00 years, 68.75% of them were males, and the incidence of AKI was 43.75%. Patients with AKI had higher levels of LPA on admission, and the more significant the increase, the higher the risk of AKI. There was a non-linear positive correlation between admission LPA and AKI, and the premeditated inflection point was 346.33 (μg/dL) through two-piecewise linear regression and recursive algorithm. Subgroup analysis identified a stronger association between admission LPA and AKI in the elder, female and medically treated patients. The incidence of CKD was 22.67% in the 2-year follow-up. Patients with subsequent CKD had higher LPA levels on admission in the follow-up cohort, and a similar interaction trend was also observed through Cox proportional—hazards model.

Conclusion

Admission LPA levels show a non-linear positive correlation with AKI and increase the risk of subsequent CKD, which is more pronounced in elderly, female, and medically treated patients.
E-Selectin/AAV2/2 Gene Therapy Alters Angiogenesis and Inflammatory Gene Profiles in Mouse Gangrene Model
Authors: Ribieras Antoine J. , Ortiz Yulexi Y. , Li Yan , Huerta Carlos T. , Le Nga , Shao Hongwei , Vazquez-Padron Roberto I. , Liu Zhao-Jun , Velazquez Omaida C.
Published: June 2022

For patients with chronic limb-threatening ischemia and limited revascularization options, alternate means for therapeutic angiogenesis and limb salvage are needed. E-selectin is a cell adhesion molecule that is critical for inflammation and neovascularization in areas of wound healing and ischemia. Here, we tested the efficacy of modifying ischemic limb tissue by intramuscular administration of E-selectin/AAV2/2 (adeno-associated virus serotype 2/2) to modulate angiogenic and inflammatory responses in a murine hindlimb gangrene model. Limb appearance, reperfusion, and functional recovery were assessed for 3 weeks after induction of ischemia. Mice receiving E-selectin/AAV2/2 gene therapy had reduced gangrene severity, increased limb and footpad perfusion, enhanced recruitment of endothelial progenitor cells, and improved performance on treadmill testing compared to control group. Histologically, E-selectin/AAV2/2 gene therapy was associated with increased vascularity and preserved myofiber integrity. E-selectin/AAV2/2 gene therapy also upregulated a panel of pro-angiogenic genes yet downregulated another group of genes associated with the inflammatory response. This novel gene therapy did not induce adverse effects on coagulability, or hematologic, hepatic, and renal function. Our findings highlight the potential of E-selectin/AAV2/2 gene therapy for improving limb perfusion and function in patients with chronic limb-threatening ischemia.
Peer Facilitators as Core Co-developers of an Online Peer Encouragement Network (OPEN2chat) for Austrian Adolescents
Authors: Mittmann Gloria , Schmalwieser Susanne Sophie , Diendorfer Tamara , Schrank Beate , Boeckle Markus
Published: June 2022

Adolescence is a crucial developmental time, and it is essential to ensure stable mental health during the transition to adulthood. Peer-to-peer networks seem to be a promising tool to support adolescents during that time. While co-development often concentrates on the end-user, this paper focuses on the peer facilitators of an online peer encouragement network (OPEN2chat), where adolescents can chat with peer facilitators about their problems. We conducted 3 group discussions with 18 peer facilitators after a testing phase to improve the process of these interactions. Thematic analysis was used to analyse the data after transcription. The four main themes were the responsibility of the peer facilitators toward their peers, especially their role of giving advice; the interaction process itself; time management; and technology aspects of the application. Including these stakeholders in the development process empowered the young people, helped eliminate problems with the application, and made the researchers more sensitive toward potential issues and emotions that peer facilitators encounter that may have been missed without a co-development process. Eliminating these problems might also help establish a better environment and support system for the actual end-users.