Contribution of Somatic Ras/Raf/Mitogen-Activated Protein Kinase Variants in the Hippocampus in Drug-Resistant Mesial Temporal Lobe Epilepsy
Authors: Khoshkhoo Sattar , Wang Yilan , Chahine Yasmine , Erson-Omay E. Zeynep , Robert Stephanie M. , Kiziltug Emre , Damisah Eyiyemisi C. , Nelson-Williams Carol , Zhu Guangya , Kong Wenna , Huang August Yue , Stronge Edward , Phillips H. Westley , Chhouk Brian H. , Bizzotto Sara , Chen Ming Hui , Adikari Thiuni N. , Ye Zimeng , Witkowski Tom , Lai Dulcie , Lee Nadine , Lokan Julie , Scheffer Ingrid E. , Berkovic Samuel F. , Haider Shozeb , Hildebrand Michael S. , Yang Edward , Gunel Murat , Lifton Richard P. , Richardson R. Mark , Blümcke Ingmar , Alexandrescu Sanda , Huttner Anita , Heinzen Erin L. , Zhu Jidong , Poduri Annapurna , DeLanerolle Nihal , Spencer Dennis D. , Lee Eunjung Alice , Walsh Christopher A. , Kahle Kristopher T. 
Published: May 2023

Importance

Mesial temporal lobe epilepsy (MTLE) is the most common focal epilepsy subtype and is often refractory to antiseizure medications. While most patients with MTLE do not have pathogenic germline genetic variants, the contribution of postzygotic (ie, somatic) variants in the brain is unknown.

Objective

To test the association between pathogenic somatic variants in the hippocampus and MTLE.

Design, Setting, and Participants

This case-control genetic association study analyzed the DNA derived from hippocampal tissue of neurosurgically treated patients with MTLE and age-matched and sex-matched neurotypical controls. Participants treated at level 4 epilepsy centers were enrolled from 1988 through 2019, and clinical data were collected retrospectively. Whole-exome and gene-panel sequencing (each genomic region sequenced more than 500 times on average) were used to identify candidate pathogenic somatic variants. A subset of novel variants was functionally evaluated using cellular and molecular assays. Patients with nonlesional and lesional (mesial temporal sclerosis, focal cortical dysplasia, and low-grade epilepsy–associated tumors) drug-resistant MTLE who underwent anterior medial temporal lobectomy were eligible. All patients with available frozen tissue and appropriate consents were included. Control brain tissue was obtained from neurotypical donors at brain banks. Data were analyzed from June 2020 to August 2022.

Exposures

Drug-resistant MTLE.

Main Outcomes and Measures

Presence and abundance of pathogenic somatic variants in the hippocampus vs the unaffected temporal neocortex.

Results

Of 105 included patients with MTLE, 53 (50.5%) were female, and the median (IQR) age was 32 (26-44) years; of 30 neurotypical controls, 11 (36.7%) were female, and the median (IQR) age was 37 (18-53) years. Eleven pathogenic somatic variants enriched in the hippocampus relative to the unaffected temporal neocortex (median [IQR] variant allele frequency, 1.92 [1.5-2.7] vs 0.3 [0-0.9]; P = .01) were detected in patients with MTLE but not in controls. Ten of these variants were in PTPN11, SOS1, KRAS, BRAF, and NF1, all predicted to constitutively activate Ras/Raf/mitogen-activated protein kinase (MAPK) signaling. Immunohistochemical studies of variant-positive hippocampal tissue demonstrated increased Erk1/2 phosphorylation, indicative of Ras/Raf/MAPK activation, predominantly in glial cells. Molecular assays showed abnormal liquid-liquid phase separation for the PTPN11 variants as a possible dominant gain-of-function mechanism.

Conclusions and Relevance

Hippocampal somatic variants, particularly those activating Ras/Raf/MAPK signaling, may contribute to the pathogenesis of sporadic, drug-resistant MTLE. These findings may provide a novel genetic mechanism and highlight new therapeutic targets for this common indication for epilepsy surgery.