bluuebunny/crossref_metadata_2025 huggingface dataset:
High Heritabilities for Antibiotic Usage Show Potential to Breed for Disease Resistance in Finishing Pigs
Authors: Gorssen Wim , Maes Dominiek , Meyermans Roel , Depuydt Jürgen , Janssens Steven , Buys Nadine
Published: July 2021

The use of antimicrobials in animal production is under public debate, mainly due to the risk of transfer of resistance to pathogenic bacteria in humans. Therefore, measures have been taken during the last few decades to reduce antibiotic usage in animals, for instance, by national monitoring programmes and by improving animal health management. Although some initiatives exist in molecular genetic selection, quantitative genetic selection of animals towards decreased antibiotic usage is an underexplored area to reduce antibiotic usage. However, this strategy could yield cumulative effects. In this study, we derived new phenotypes from on-farm parenteral antibiotic records from commercially grown crossbred finishing pigs used in the progeny test of Piétrain terminal sires to investigate the heritability of antibiotics usage. Parenteral antibiotic records, production parameters and pedigree records of 2238 full-sib pens from two experimental farms in Belgium between 2014 and 2020 were analysed. Heritability estimates were moderate (18–44%) for phenotypes derived from all antibiotic treatments, and low (1–15%) for phenotypes derived from treatments against respiratory diseases only. Moreover, genetic correlations between these new phenotypes and mortality were low to moderate (0.08–0.60) and no strong adverse genetic correlations with production traits were found. The high heritabilities and favourable genetic correlations suggest these new phenotypes, derived from on-farm antibiotics records, to be promising for inclusion in future pig breeding programs to breed for a decrease in antibiotics usage.
Ecological Response in the Integrated Process of Biostimulation and Bioaugmentation of Diesel-Contaminated Soil
Authors: Li Xiaosen , Chen Yakui , Du Xianyuan , Zheng Jin , Lu Diannan , Liu Zheng
Published: July 2021

The study applied microbial molecular biological techniques to show that 2.5% to 3.0% (w/w) of diesel in the soil reduced the types and number of bacteria in the soil and destroyed the microbial communities responsible for the nitrogen cycle. In the meantime, the alkane degradation gene alkB and polycyclic aromatic hydrocarbons (PAHs) degradation gene nah evolved in the contaminated soil. We evaluated four different remediation procedures, in which the biostimulation-bioaugmentation joint process reached the highest degradation rate of diesel, 59.6 ± 0.25% in 27 days. Miseq sequencing and quantitative polymerase chain reaction (qPCR) showed that compared with uncontaminated soil, repaired soil provides abundant functional genes related to soil nitrogen cycle, and the most significant lifting effect on diesel degrading bacteria γ-proteobacteria. Quantitative analysis of degrading functional genes shows that degrading bacteria can be colonized in the soil. Gas chromatography-mass spectrometry (GC-MS) results show that the components remaining in the soil after diesel degradation are alcohol, lipids and a small amount of fatty amine compounds, which have very low toxicity to plants. In an on-site remediation experiment, the diesel content decreased from 2.7% ± 0.3 to 1.12% ± 0.1 after one month of treatment. The soil physical and chemical properties returned to normal levels, confirming the practicability of the biosimulation-bioaugmentation jointed remediation process.
Modal Verification and Strength Analysis of Bladed Rotors of Turbine in Rated Working Conditions
Authors: Lin Chang-Sheng , Chiang Hung-Tse , Hsu Chuan-Hsing , Lin Ming-Hsien , Liu Jui-Kai , Bai Chi-Jeng
Published: July 2021

The Top-pressure Recovery Turbine (TRT) uses the blast furnace gas generated in the iron and steel manufacturing process to push the turbine which drives the generator to generate electricity, and the generated electric energy is supplied to in-plant equipment. In this paper, we investigate the aerodynamic force, centrifugal force, and maximum stress on the structure of the TRT rotor in rated working conditions and the positions of occurrence using the Finite Element Method (FEM), as well as discuss the dynamic characteristics of bladed disks during TRT operation through Campbell and SAFE diagrams. To confirm the effectiveness of the finite element models, the mode shapes and natural frequencies in the FEA-based modal analysis of the TRT rotor will be captured and compared with those of the practical structures through the Experimented Modal Analysis (EMA). To verify the agreement between the mode shapes of the finite element analysis and those of the actual structure, the Modal Assurance Criterion (MAC) is introduced here to confirm the reliability of the finite element model. The stress distribution on the structure in the rotation is obtained by centrifugal force analysis. The TRT rotor is driven as the blast furnace top pressure pushes the moving blade; when the rotor rotates, the moving blade bears centrifugal and periodic aerodynamic forces. The stress distribution is investigated on the structure when these forces act simultaneously using aerodynamic analysis. To discuss whether the bladed disks will resonate with the external force under the operating conditions, Campbell and SAFE diagrams are used for evaluation, and the modal parameters obtained from the EMA are used to estimate the strength and durability of the blades. According to the analysis results when the TRT rotor is in working conditions, the fatigue failure may occur at the maximum stress existing on the dovetail slot.
Gelatin-Based Film Integrated with Copper Sulfide Nanoparticles for Active Packaging Applications
Authors: Roy Swarup , Rhim Jong-Whan
Published: July 2021

Gelatin-based multifunctional composite films were prepared by reinforcing various amounts of copper sulfide nanoparticles (CuSNP, 0.0, 0.5, 1.0, and 2.0 wt %), and the effect of CuSNP on the film was evaluated by analyzing its physical and antibacterial properties. CuSNP makes a compatible film with gelatin. The inclusion of CuSNP significantly enhanced the UV blocking, mechanical strength, and water vapor barrier properties of the gelatin film. The inclusion of CuSNP of 1.0 wt % or less did not affect the transparency of the gelatin film. When 2.0 wt % of CuSNP was mixed, the hydrophilicity of the gelatin film did not change noticeably, but its thermal properties slightly increased. Moreover, the gelatin/CuSNP composite film presented effective antibacterial activity against E. coli and some activity against L. monocytogenes. Gelatin/CuSNP composite films with better functional and physical properties can be used for food packaging or biomedical applications.
Saint John Baptistery in Florence (Italy): Studies for Conservation of the External Marble Cladding
Authors: Coli Massimo , Ciuffreda Anna Livia , Donigaglia Tessa , Bencaster Attilio , Caciagli Samuele , Agostini Beatrice , Iandelli Niccolò
Published: July 2021

Saint John’s Baptistery in Florence (Italy), dating back to the XI century, represents one of the most outstanding historical buildings in the city, and has been under the UNESCO patronage as Cultural Heritage since 1982. In recent years, in the frame of a conservation project, detailed studies and mapping of all the tiles covering the Baptistery have been developed. Based on a laser-scan survey, a detailed wireframe model of all the external sides, reporting all the tiles and decor, has been developed. This model was implemented into a 2D-GIS, georeferenced in real scale and spatial position. An in situ survey of all the tiles, ashlars, inlays and columns, made in contradiction by experts in historical ornamental stones, allowed the recognition of several types of marble in place. All these marbles have been analyzed and characterized as geometric, geological and historical data, and the information implemented into a GIS for obtaining a spatial geodatabase representing a “box” to store all information achieved. All these data are manageable by web through smartphone, tablet and PC for querying or updating, thus representing an effective management tool for further conservation of such important historical cultural buildings.
Theoretical and Numerical Solution for the Bending and Frequency Response of Graphene Reinforced Nanocomposite Rectangular Plates
Authors: Safarpour Mehran , Forooghi Ali , Dimitri Rossana , Tornabene Francesco
Published: July 2021

In this work, we study the vibration and bending response of functionally graded graphene platelets reinforced composite (FG-GPLRC) rectangular plates embedded on different substrates and thermal conditions. The governing equations of the problem along with boundary conditions are determined by employing the minimum total potential energy and Hamilton’s principle, within a higher-order shear deformation theoretical setting. The problem is solved both theoretically and numerically by means of a Navier-type exact solution and a generalized differential quadrature (GDQ) method, respectively, whose results are successfully validated against the finite element predictions performed in the commercial COMSOL code, and similar outcomes available in the literature. A large parametric study is developed to check for the sensitivity of the response to different foundation properties, graphene platelets (GPL) distribution patterns, volume fractions of the reinforcing phase, as well as the surrounding environment and boundary conditions, with very interesting insights from a scientific and design standpoint.
Study on Solar Radiation and the Extreme Thermal Effect on Concrete Box Girder Bridges
Authors: Lu Yao , Li Dejian , Wang Kai , Jia Shiwei
Published: July 2021

Thermal effect is an essential factor in the durability and safety of concrete bridges. Therefore, this paper mainly studied the concrete bridge box girder temperature distribution and thermal effect under solar radiation and the thermal load. With a concrete rigid frame bridge as the engineering background, the temperature distribution of the box girder on a clear summer day was observed. Then, according to the solar physics and heat transfer theory, the different surfaces of the box girder cross-section are classified based on the heat transfer conditions, and the variation of solar radiation on different surfaces is investigated. The temperature field of the box girder is simulated by ANSYS. To obtain the extreme thermal condition, the meteorological data of the bridge site from 1990 to 2020 are collected. The data are fitted by generalized extreme value distribution to obtain the extreme temperature and average wind factors in the bridge design lifetime. Combined with the solar radiation, temperature, and wind factors, the extreme thermal condition of the concrete box girder is obtained. Lastly, the thermal effect of the box girder under the extreme condition is analyzed, and the thermal stress is compared with the allowable stress in the design code. The results show that the girder temperature difference is closely related to the solar radiation intensity and heat transfer conditions, and the solar radiation intensity is the more critical factor. The tensile stress caused by the extreme thermal load is more significant than the design strength value in the girder cross-section. The results also provide a method to obtain the extreme thermal condition and evaluate the impact of the thermal effect on concrete box girder bridges.
Precipitation Regime Changes at Four Croatian Meteorological Stations
Authors: Bonacci Ognjen , Andrić Ivo , Vrsalović Adrijana , Bonacci Duje
Published: July 2021

The article analyses the values of daily, monthly and annual precipitation measured during the period 1948–2019 at the following four stations: (1) Split, (2) Hvar, (3) Lastovo and (4) Zagreb. The first three stations are located in a Mediterranean climate, while the station in Zagreb is located in a continental climate. The aim of the performed analyses is to detect non-stationarity (trends, jumps, and seasonality) in the precipitation regime at three-time scales (day, month, and year) over the period of the last 72 years (1948–2020). Numerous previous analyses at all four stations showed statistically significant increases in air temperature, which were particularly amplified in the late 1980s by the effect of global warming. Expressed as a percentage of the total annual precipitation at all four analysed locations, the presence of an increasing trend was calculated. The analyses carried out in this work showed that there was a redistribution of precipitation during the year, a decrease in the number of days with precipitation and an intensification of precipitation in both climatic regions. Over the past 73 years, the number of days with precipitation per year has slowly decreased. The number of days with intense precipitation, P ≥ 32.0 mm, has become more frequent.
A Fully Relativistic Approach to Photon Scattering and Photoionization of Alkali Atoms
Authors: Singor Adam , Fursa Dmitry , Bray Igor , McEachran Robert
Published: July 2021

A fully relativistic approach to calculating photoionization and photon-atom scattering cross sections for quasi one-electron atoms is presented. An extensive set of photoionization cross sections have been calculated for alkali atoms: lithium, sodium, potassium, rubidium and cesium. The importance of relativistic effects and core polarization on the depth and position of the Cooper minimum in the photoionization cross section is investigated. Good agreement was found with previous Dirac-based B-spline R-matrix calculations of Zatsarinny and Tayal and recent experimental results.
The Role of Metabolic Engineering Technologies for the Production of Fatty Acids in Yeast
Authors: Ullah Numan , Shahzad Khuram , Wang Mengzhi
Published: July 2021

Metabolic engineering is a cutting-edge field that aims to produce simple, readily available, and inexpensive biomolecules by applying different genetic engineering and molecular biology techniques. Fatty acids (FAs) play an important role in determining the physicochemical properties of membrane lipids and are precursors of biofuels. Microbial production of FAs and FA-derived biofuels has several advantages in terms of sustainability and cost. Conventional yeast Saccharomyces cerevisiae is one of the models used for FA synthesis. Several genetic manipulations have been performed to enhance the citrate accumulation and its conversation into acetyl-CoA, a precursor for FA synthesis. Success has been achieved in producing different chemicals, including FAs and their derivatives, through metabolic engineering. However, several hurdles such as slow growth rate, low oleaginicity, and cytotoxicity are still need to be resolved. More robust research needs to be conducted on developing microbes capable of resisting diverse environments, chemicals, and cost-effective feed requirements. Redesigning microbes to produce FAs with cutting-edge synthetic biology and CRISPR techniques can solve these problems. Here, we reviewed the technological progression of metabolic engineering techniques and genetic studies conducted on S. cerevisiae, making it suitable as a model organism and a great candidate for the production of biomolecules, especially FAs.
Probiotics, Prebiotics and Postbiotics on Mitigation of Depression Symptoms: Modulation of the Brain–Gut–Microbiome Axis
Authors: Chudzik Agata , Orzyłowska Anna , Rola Radosław , Stanisz Greg J.
Published: July 2021

The brain–gut–microbiome axis is a bidirectional communication pathway between the gut microbiota and the central nervous system. The growing interest in the gut microbiota and mechanisms of its interaction with the brain has contributed to the considerable attention given to the potential use of probiotics, prebiotics and postbiotics in the prevention and treatment of depressive disorders. This review discusses the up-to-date findings in preclinical and clinical trials regarding the use of pro-, pre- and postbiotics in depressive disorders. Studies in rodent models of depression show that some of them inhibit inflammation, decrease corticosterone level and change the level of neurometabolites, which consequently lead to mitigation of the symptoms of depression. Moreover, certain clinical studies have indicated improvement in mood as well as changes in biochemical parameters in patients suffering from depressive disorders.
Roles of XBP1s in Transcriptional Regulation of Target Genes
Authors: Park Sung-Min , Kang Tae-Il , So Jae-Seon
Published: July 2021

The spliced form of X-box binding protein 1 (XBP1s) is an active transcription factor that plays a vital role in the unfolded protein response (UPR). Under endoplasmic reticulum (ER) stress, unspliced Xbp1 mRNA is cleaved by the activated stress sensor IRE1α and converted to the mature form encoding spliced XBP1 (XBP1s). Translated XBP1s migrates to the nucleus and regulates the transcriptional programs of UPR target genes encoding ER molecular chaperones, folding enzymes, and ER-associated protein degradation (ERAD) components to decrease ER stress. Moreover, studies have shown that XBP1s regulates the transcription of diverse genes that are involved in lipid and glucose metabolism and immune responses. Therefore, XBP1s has been considered an important therapeutic target in studying various diseases, including cancer, diabetes, and autoimmune and inflammatory diseases. XBP1s is involved in several unique mechanisms to regulate the transcription of different target genes by interacting with other proteins to modulate their activity. Although recent studies discovered numerous target genes of XBP1s via genome-wide analyses, how XBP1s regulates their transcription remains unclear. This review discusses the roles of XBP1s in target genes transcriptional regulation. More in-depth knowledge of XBP1s target genes and transcriptional regulatory mechanisms in the future will help develop new therapeutic targets for each disease.
Building a Cardiovascular Disease Prediction Model for Smartwatch Users Using Machine Learning: Based on the Korea National Health and Nutrition Examination Survey
Authors: Kim Min-Jeong
Published: July 2021

Smartwatches have the potential to support health care in everyday life by supporting self-monitoring of health conditions and personal activities. This paper aims to develop a model that predicts the prevalence of cardiovascular disease using health-related data that can be easily measured by smartwatch users. To this end, the data corresponding to the health-related data variables provided by the smartwatch are selected from the Korea National Health and Nutrition Examination Survey. To classify the prevalence of cardiovascular disease with these selected variables, we apply logistic regression, artificial neural network, and support vector machine among machine learning classification techniques, and compare the appropriateness of the algorithm through classification performance indicators. The prediction model using support vector machine showed the highest accuracy. Next, we analyze which structures or parameters of the support vector machine contribute to increasing accuracy and derive the importance of input variables. Since it is very important to diagnose cardiovascular disease early correctly, we expect that this model will be very useful if there is a tool to predict whether cardiovascular disease develops or not.
Effects of Cognicise-Neurofeedback on Health Locus of Control, Depression, and Quantitative Electroencephalography Alpha Asymmetry in Elderly Women
Authors: Weon Heewook , Yoo Jieun , Yu Jumhwa , Park Miso , Son Haekyoung
Published: July 2021

Background: With an increase in the aged population, there is a growing concern regarding the care of the elderly. This study aims to identify effects of cognicise-neurofeedback on health locus of control, depression, and quantitative electroencephalography (QEEG) alpha asymmetry in elderly women. Methods: A quasi-experimental control group pre-test–post-test design was used. Korean women aged 65 years or over at a senior welfare center were randomly allocated to the control (n = 12) or experimental (n = 19) groups from July to October 2019. The intervention consisted of cognicise (exercise with intensified cognitive activity) and neurofeedback twice a week for 10 weeks. The locus of control and depression were measured via self-reported questionnaires. QEEG alpha asymmetry was measured using BrainMaster. Results: Depression significantly decreased in the experimental group (t = 4.113, p = 0.001), while internality in the locus of control significantly decreased in the control group (t = 3.023, p = 0.012). On the other hand, QEEG alpha asymmetry index differences in F3-F4 between the pre-test and post-test were not significant in the experimental group (t = 0.491, p = 0.629) or control group (t = 0.413, p = 0.678). Conclusions: Due to the coronavirus disease (COVID-19) pandemic, contact between the elderly and healthcare workers in the clinical practice field has become more restricted. These findings can help decrease negative emotions among elderly women in the community based on an integrated neuroscientific approach.
Thermal Performance of Double-Pane Lightweight Steel Framed Walls with and without a Reflective Foil
Authors: Santos Paulo , Ribeiro Telmo
Published: July 2021

One strategy to increase energy efficiency of buildings could be the reduction of undesirable heat losses by mitigating the heat transfer mechanisms across the building envelope. The use of thermal insulation is the simplest and most straightforward way to promote thermal resistance of building elements by reducing the heat transfer by conduction. However, whenever there is an air cavity, radiation heat transfer could be also very relevant. The use of thermal reflective insulation materials inside the air gaps of building elements is likewise an effective way to increase thermal resistance without increasing weight and wall thickness. Some additional advantages are its low-cost and easy installation. In this work, the performance of a thermal reflective insulation system, constituted by an aluminium foil placed inside an air cavity between a double pane lightweight steel framed (LSF) partition, is experimentally evaluated for different air gap thicknesses, ranging from 0 mm up to 50 mm, with a step increment of 10 mm. We found a maximum thermal resistance improvement of the double pane LSF walls due to the reflective foil of around +0.529 m2∙°C/W (+21%). The measurements of the R-values were compared with predictions provided by simplified models (CEN and NFRC 100). Both models were able to predict with reasonable accuracy (around ±5%) the thermal behaviour of the air cavities within the evaluated double pane LSF walls.
The Genetic Analyses of French Canadians of Quebec Facilitate the Characterization of New Cancer Predisposing Genes Implicated in Hereditary Breast and/or Ovarian Cancer Syndrome Families
Authors: Fierheller Caitlin T. , Alenezi Wejdan M. , Tonin Patricia N.
Published: July 2021

The French Canadian population of the province of Quebec has been recognized for its contribution to research in medical genetics, especially in defining the role of heritable pathogenic variants in cancer predisposing genes. Multiple carriers of a limited number of pathogenic variants in BRCA1 and BRCA2, the major risk genes for hereditary breast and/or ovarian cancer syndrome families, have been identified in French Canadians, which is in stark contrast to the array of over 2000 different pathogenic variants reported in each of these genes in other populations. As not all such cancer syndrome families are explained by BRCA1 and BRCA2, newly proposed gene candidates identified in other populations have been investigated for their role in conferring risk in French Canadian cancer families. For example, multiple carriers of distinct variants were identified in PALB2 and RAD51D. The unique genetic architecture of French Canadians has been attributed to shared ancestry due to common ancestors of early settlers of this population with origins mainly from France. In this review, we discuss the merits of genetically characterizing cancer predisposing genes in French Canadians of Quebec. We focused on genes that have been implicated in hereditary breast and/or ovarian cancer syndrome families as they have been the most thoroughly characterized cancer syndromes in this population. We describe how genetic analyses of French Canadians have facilitated: (i) the classification of variants in BRCA1 and BRCA2; (ii) the identification and classification of variants in newly proposed breast and/or ovarian cancer predisposing genes; and (iii) the identification of a new breast cancer predisposing gene candidate, RECQL. The genetic architecture of French Canadians provides a unique opportunity to evaluate new candidate cancer predisposing genes regardless of the population in which they were identified.
Establishment and Validation of CyberKnife Irradiation in a Syngeneic Glioblastoma Mouse Model
Authors: Jelgersma Claudius , Senger Carolin , Kluge Anne Kathrin , Janas Anastasia , Nieminen-Kelhä Melina , Kremenetskaia Irina , Mueller Susanne , Brandenburg Susan , Loebel Franziska , Tinhofer Ingeborg , Conti Alfredo , Budach Volker , Vajkoczy Peter , Acker Gueliz
Published: July 2021

CyberKnife stereotactic radiosurgery (CK-SRS) precisely delivers radiation to intracranial tumors. However, the underlying radiobiological mechanisms at high single doses are not yet fully understood. Here, we established and evaluated the early radiobiological effects of CK-SRS treatment at a single dose of 20 Gy after 15 days of tumor growth in a syngeneic glioblastoma-mouse model. Exact positioning was ensured using a custom-made, non-invasive, and trackable frame. One superimposed target volume for the CK-SRS planning was created from the fused tumor volumes obtained from MRIs prior to irradiation. Dose calculation and delivery were planned using a single-reference CT scan. Six days after irradiation, tumor volumes were measured using MRI scans, and radiobiological effects were assessed using immunofluorescence staining. We found that CK-SRS treatment reduced tumor volume by approximately 75%, impaired cell proliferation, diminished tumor vasculature, and increased immune response. The accuracy of the delivered dose was demonstrated by staining of DNA double-strand breaks in accordance with the planned dose distribution. Overall, we confirmed that our proposed setup enables the precise irradiation of intracranial tumors in mice using only one reference CT and superimposed MRI volumes. Thus, our proposed mouse model for reproducible CK-SRS can be used to investigate radiobiological effects and develop novel therapeutic approaches.
Hypoxia in Lung Cancer Management: A Translational Approach
Authors: Ancel Julien , Perotin Jeanne-Marie , Dewolf Maxime , Launois Claire , Mulette Pauline , Nawrocki-Raby Béatrice , Dalstein Véronique , Gilles Christine , Deslée Gaëtan , Polette Myriam , Dormoy Valérian
Published: July 2021

Lung cancer represents the first cause of death by cancer worldwide and remains a challenging public health issue. Hypoxia, as a relevant biomarker, has raised high expectations for clinical practice. Here, we review clinical and pathological features related to hypoxic lung tumours. Secondly, we expound on the main current techniques to evaluate hypoxic status in NSCLC focusing on positive emission tomography. We present existing alternative experimental approaches such as the examination of circulating markers and highlight the interest in non-invasive markers. Finally, we evaluate the relevance of investigating hypoxia in lung cancer management as a companion biomarker at various lung cancer stages. Hypoxia could support the identification of patients with higher risks of NSCLC. Moreover, the presence of hypoxia in treated tumours could help clinicians predict a worse prognosis for patients with resected NSCLC and may help identify patients who would benefit potentially from adjuvant therapies. Globally, the large quantity of translational data incites experimental and clinical studies to implement the characterisation of hypoxia in clinical NSCLC management.
MOUSSE: Multi-Omics Using Subject-Specific SignaturEs
Authors: Fiorentino Giuseppe , Visintainer Roberto , Domenici Enrico , Lauria Mario , Marchetti Luca
Published: July 2021

High-throughput technologies make it possible to produce a large amount of data representing different biological layers, examples of which are genomics, proteomics, metabolomics and transcriptomics. Omics data have been individually investigated to understand the molecular bases of various diseases, but this may not be sufficient to fully capture the molecular mechanisms and the multilayer regulatory processes underlying complex diseases, especially cancer. To overcome this problem, several multi-omics integration methods have been introduced but a commonly agreed standard of analysis is still lacking. In this paper, we present MOUSSE, a novel normalization-free pipeline for unsupervised multi-omics integration. The main innovations are the use of rank-based subject-specific signatures and the use of such signatures to derive subject similarity networks. A separate similarity network was derived for each omics, and the resulting networks were then carefully merged in a way that considered their informative content. We applied it to analyze survival in ten different types of cancer. We produced a meaningful clusterization of the subjects and obtained a higher average classification score than ten state-of-the-art algorithms tested on the same data. As further validation, we extracted from the subject-specific signatures a list of relevant features used for the clusterization and investigated their biological role in survival. We were able to verify that, according to the literature, these features are highly involved in cancer progression and differential survival.
Store-Operated Calcium Channels Control Proliferation and Self-Renewal of Cancer Stem Cells from Glioblastoma
Authors: Terrié Elodie , Déliot Nadine , Benzidane Yassine , Harnois Thomas , Cousin Laëtitia , Bois Patrick , Oliver Lisa , Arnault Patricia , Vallette François , Constantin Bruno , Coronas Valérie
Published: July 2021

Glioblastoma is the most frequent and deadly form of primary brain tumors. Despite multimodal treatment, more than 90% of patients experience tumor recurrence. Glioblastoma contains a small population of cells, called glioblastoma stem cells (GSC) that are highly resistant to treatment and endowed with the ability to regenerate the tumor, which accounts for tumor recurrence. Transcriptomic studies disclosed an enrichment of calcium (Ca2+) signaling transcripts in GSC. In non-excitable cells, store-operated channels (SOC) represent a major route of Ca2+ influx. As SOC regulate the self-renewal of adult neural stem cells that are possible cells of origin of GSC, we analyzed the roles of SOC in cultures of GSC previously derived from five different glioblastoma surgical specimens. Immunoblotting and immunocytochemistry experiments showed that GSC express Orai1 and TRPC1, two core SOC proteins, along with their activator STIM1. Ca2+ imaging demonstrated that SOC support Ca2+ entries in GSC. Pharmacological inhibition of SOC-dependent Ca2+ entries decreased proliferation, impaired self-renewal, and reduced expression of the stem cell marker SOX2 in GSC. Our data showing the ability of SOC inhibitors to impede GSC self-renewal paves the way for a strategy to target the cells considered responsible for conveying resistance to treatment and tumor relapse.
Dry Reforming of Methane Using Ni Catalyst Supported on ZrO2: The Effect of Different Sources of Zirconia
Authors: Ibrahim Ahmed , Fakeeha Anis , Abasaeed Ahmed , Al-Fatesh Ahmed
Published: July 2021

Dry reforming of methane (DRM) has a substantial potential to provide a cost-effective process and in reducing greenhouse gases. Its application has been hindered by carbon deposition and instability problems. The use of an appropriate catalyst is influenced by the support type. The objective of this investigation is to elucidate the effect of different sources of ZrO2 support. Four kinds of ZrO2, namely RC-100 and Z-3215, MKnano, and ELTN were acquired from Japan, Canada, and China, respectively. The catalyst samples were analyzed by BET, XRD, TPR, TPD, TEM, TGA, TPO, FT-IR, and Raman. The analysis of the structural properties displayed that all Ni-supported catalysts, regardless of their source, are mesoporous and that 5Ni-RC-100 possessed the highest BET surface area of 17.7 m2/g and 5Ni-MKnano had the lowest value of BET 3.16 m2/g. In the TPD and TEM analysis, the 5Ni-RC-100 catalyst presented the highest intensity of basicity and the minimum average particle size of 3.35 nm, respectively. The 5Ni-RC-100 catalyst outperformed 5Ni-ELTN by exhibiting 44% higher CH4 conversion; however, 5Ni-RC-100 gave the highest weight loss in the TGA analysis of 66%.
Investigation of ZrMnFe/Sepiolite Catalysts on Toluene Degradation in a One-Stage Plasma-Catalysis System
Authors: Liu Jianqi , Liu Xin , Chen Jiayao , Li Xianying , Ma Tianpeng , Zhong Fangchuan
Published: July 2021

Toluene removal by double dielectric barrier charge (DDBD) plasma combined with a ZrMnFe/Sepiolite (SEP) catalyst was investigated and compared with the results from Fe/SEP, Mn/SEP and MnFe/SEP ones. All the catalysts were prepared by the impregnation method and characterized by XRD, BET, ICP, SEM, TEM, H2-TPR and XPS. The effect of catalysts on toluene degradation efficiency, carbon balance, CO2 selectivity and residual O3 concentration was studied. The experimental results indicated that the ZrMnFe/SEP catalyst presented the best catalytic performance. This is because of the high content of lattice oxygen contained in its surface, owing to the addition of Zr. When the SIE was 740 J/L, the highest toluene removal efficiency (87%), carbon balance (93%) and CO2 selectivity (51%) were obtained. The ZrMnFe/SEP catalyst had a better ozone inhibition effect than other catalysts. The catalyst has good stability, which the toluene removal efficiency, carbon balance and CO2 selectivity did not decrease significantly after 36 h of work at a constant energy density. The results indicated that the ZrMnFe/SEP catalyst is an efficient catalyst for degradation of toluene by plasma-catalyst measures.
Role of Alternative Splicing in Regulating Host Response to Viral Infection
Authors: Liao Kuo-Chieh , Garcia-Blanco Mariano A.
Published: July 2021

The importance of transcriptional regulation of host genes in innate immunity against viral infection has been widely recognized. More recently, post-transcriptional regulatory mechanisms have gained appreciation as an additional and important layer of regulation to fine-tune host immune responses. Here, we review the functional significance of alternative splicing in innate immune responses to viral infection. We describe how several central components of the Type I and III interferon pathways encode spliced isoforms to regulate IFN activation and function. Additionally, the functional roles of splicing factors and modulators in antiviral immunity are discussed. Lastly, we discuss how cell death pathways are regulated by alternative splicing as well as the potential role of this regulation on host immunity and viral infection. Altogether, these studies highlight the importance of RNA splicing in regulating host–virus interactions and suggest a role in downregulating antiviral innate immunity; this may be critical to prevent pathological inflammation.
Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (Glycine max) Leaves
Authors: Fatima Anis , Kataria Sunita , Agrawal Ashish Kumar , Singh Balwant , Kashyap Yogesh , Jain Meeta , Brestic Marian , Allakhverdiev Suleyman I. , Rastogi Anshu
Published: July 2021

The combined response of exclusion of solar ultraviolet radiation (UV-A+B and UV-B) and static magnetic field (SMF) pre-treatment of 200 mT for 1 h were studied on soybean (Glycine max) leaves using synchrotron imaging. The seeds of soybean with and without SMF pre-treatment were sown in nursery bags kept in iron meshes where UV-A+B (280–400 nm) and UV-B (280–315 nm) from solar radiation were filtered through a polyester filters. Two controls were planned, one with polythene filter controls (FC)- which allows all the UV (280–400 nm); the other control had no filter used (open control-OC). Midrib regions of the intact third trifoliate leaves were imaged using the phase-contrast imaging technique at BL-4, Indus-2 synchrotron radiation source. The solar UV exclusion results suggest that ambient UV caused a reduction in leaf growth which ultimately reduced the photosynthesis in soybean seedlings, while SMF treatment caused enhancement of leaf growth along with photosynthesis even under the presence of ambient UV-B stress. The width of midrib and second-order veins, length of the second-order veins, leaf vein density, and the density of third-order veins obtained from the quantitative image analysis showed an enhancement in the leaves of plants that emerged from SMF pre-treated seeds as compared to untreated ones grown in open control and filter control conditions (in the presence of ambient UV stress). SMF pre-treated seeds along with UV-A+B and UV-B exclusion also showed significant enhancements in leaf parameters as compared to the UV excluded untreated leaves. Our results suggested that SMF-pretreatment of seeds diminishes the ambient UV-induced adverse effects on soybean.
Modeling Hepatotropic Viral Infections: Cells vs. Animals
Authors: Khoshdel-Rad Niloofar , Zahmatkesh Ensieh , Bikmulina Polina , Peshkova Maria , Kosheleva Nastasia , Bezrukov Evgeny A. , Sukhanov Roman B. , Solovieva Anna , Shpichka Anastasia , Timashev Peter , Vosough Massoud
Published: July 2021

The lack of an appropriate platform for a better understanding of the molecular basis of hepatitis viruses and the absence of reliable models to identify novel therapeutic agents for a targeted treatment are the two major obstacles for launching efficient clinical protocols in different types of viral hepatitis. Viruses are obligate intracellular parasites, and the development of model systems for efficient viral replication is necessary for basic and applied studies. Viral hepatitis is a major health issue and a leading cause of morbidity and mortality. Despite the extensive efforts that have been made on fundamental and translational research, traditional models are not effective in representing this viral infection in a laboratory. In this review, we discuss in vitro cell-based models and in vivo animal models, with their strengths and weaknesses. In addition, the most important findings that have been retrieved from each model are described.
M2 Muscarinic Receptor Activation Impairs Mitotic Progression and Bipolar Mitotic Spindle Formation in Human Glioblastoma Cell Lines
Authors: Di Bari Maria , Tombolillo Vanessa , Alessandrini Francesco , Guerriero Claudia , Fiore Mario , Asteriti Italia Anna , Castigli Emilia , Sciaccaluga Miriam , Guarguaglini Giulia , Degrassi Francesca , Tata Ada Maria
Published: July 2021

Background: Glioblastoma multiforme (GBM) is characterized by several genetic abnormalities, leading to cell cycle deregulation and abnormal mitosis caused by a defective checkpoint. We previously demonstrated that arecaidine propargyl ester (APE), an orthosteric agonist of M2 muscarinic acetylcholine receptors (mAChRs), arrests the cell cycle of glioblastoma (GB) cells, reducing their survival. The aim of this work was to better characterize the molecular mechanisms responsible for this cell cycle arrest. Methods: The arrest of cell proliferation was evaluated by flow cytometry analysis. Using immunocytochemistry and time-lapse analysis, the percentage of abnormal mitosis and aberrant mitotic spindles were assessed in both cell lines. Western blot analysis was used to evaluate the modulation of Sirtuin2 and acetylated tubulin—factors involved in the control of cell cycle progression. Results: APE treatment caused arrest in the M phase, as indicated by the increase in p-HH3 (ser10)-positive cells. By immunocytochemistry, we found a significant increase in abnormal mitoses and multipolar mitotic spindle formation after APE treatment. Time-lapse analysis confirmed that the APE-treated GB cells were unable to correctly complete the mitosis. The modulated expression of SIRT2 and acetylated tubulin in APE-treated cells provides new insights into the mechanisms of altered mitotic progression in both GB cell lines. Conclusions: Our data show that the M2 agonist increases aberrant mitosis in GB cell lines. These results strengthen the idea of considering M2 acetylcholine receptors a novel promising therapeutic target for the glioblastoma treatment.
Pathophysiological Effects of Overactive STIM1 on Murine Muscle Function and Structure
Authors: Silva-Rojas Roberto , Charles Anne-Laure , Djeddi Sarah , Geny Bernard , Laporte Jocelyn , Böhm Johann
Published: July 2021

Store-operated Ca2+ entry (SOCE) is a ubiquitous mechanism regulating extracellular Ca2+ entry to control a multitude of Ca2+-dependent signaling pathways and cellular processes. SOCE relies on the concerted activity of the reticular Ca2+ sensor STIM1 and the plasma membrane Ca2+ channel ORAI1, and dysfunctions of these key factors result in human pathologies. STIM1 and ORAI1 gain-of-function (GoF) mutations induce excessive Ca2+ influx through SOCE over-activation, and cause tubular aggregate myopathy (TAM) and Stormorken syndrome (STRMK), two overlapping disorders characterized by muscle weakness and additional multi-systemic signs affecting growth, platelets, spleen, skin, and intellectual abilities. In order to investigate the pathophysiological effect of overactive SOCE on muscle function and structure, we combined transcriptomics with morphological and functional studies on a TAM/STRMK mouse model. Muscles from Stim1R304W/+ mice displayed aberrant expression profiles of genes implicated in Ca2+ handling and excitation-contraction coupling (ECC), and in vivo investigations evidenced delayed muscle contraction and relaxation kinetics. We also identified signs of reticular stress and abnormal mitochondrial activity, and histological and respirometric analyses on muscle samples revealed enhanced myofiber degeneration associated with reduced mitochondrial respiration. Taken together, we uncovered a molecular disease signature and deciphered the pathomechanism underlying the functional and structural muscle anomalies characterizing TAM/STRMK.
Group V Phospholipase A2 Mediates Endothelial Dysfunction and Acute Lung Injury Caused by Methicillin-Resistant Staphylococcus Aureus
Authors: Htwe Yu Maw , Wang Huashan , Belvitch Patrick , Meliton Lucille , Bandela Mounica , Letsiou Eleftheria , Dudek Steven M.
Published: July 2021

Lung endothelial dysfunction is a key feature of acute lung injury (ALI) and clinical acute respiratory distress syndrome (ARDS). Previous studies have identified the lipid-generating enzyme, group V phospholipase A2 (gVPLA2), as a mediator of lung endothelial barrier disruption and inflammation. The current study aimed to determine the role of gVPLA2 in mediating lung endothelial responses to methicillin-resistant Staphylococcus aureus (MRSA, USA300 strain), a major cause of ALI/ARDS. In vitro studies assessed the effects of gVPLA2 inhibition on lung endothelial cell (EC) permeability after exposure to heat-killed (HK) MRSA. In vivo studies assessed the effects of intratracheal live or HK-MRSA on multiple indices of ALI in wild-type (WT) and gVPLA2-deficient (KO) mice. In vitro, HK-MRSA increased gVPLA2 expression and permeability in human lung EC. Inhibition of gVPLA2 with either the PLA2 inhibitor, LY311727, or with a specific monoclonal antibody, attenuated the barrier disruption caused by HK-MRSA. LY311727 also reduced HK-MRSA-induced permeability in mouse lung EC isolated from WT but not gVPLA2-KO mice. In vivo, live MRSA caused significantly less ALI in gVPLA2 KO mice compared to WT, findings confirmed by intravital microscopy assessment in HK-MRSA-treated mice. After targeted delivery of gVPLA2 plasmid to lung endothelium using ACE antibody-conjugated liposomes, MRSA-induced ALI was significantly increased in gVPLA2-KO mice, indicating that lung endothelial expression of gVPLA2 is critical in vivo. In summary, these results demonstrate an important role for gVPLA2 in mediating MRSA-induced lung EC permeability and ALI. Thus, gVPLA2 may represent a novel therapeutic target in ALI/ARDS caused by bacterial infection.
Electrochemical Switching of First-Generation Donor-Acceptor Stenhouse Adducts (DASAs): An Alternative Stimulus for Triene Cyclisation
Authors: Shepherd Nicholas D. , Moore Harrison S. , Beves Jonathon E. , D’Alessandro Deanna M.
Published: July 2021

Donor-acceptor Stenhouse adducts (DASAs) are a photo-switch class that undergoes triene cyclisation in response to visible light. Herein, electrochemical oxidation is demonstrated as an effective alternative stimulus for the triene cyclisation commonly associated with photo-switching.
Antibiotic Resistance Patterns of Uropathogens Causing Urinary Tract Infections in Children with Congenital Anomalies of Kidney and Urinary Tract
Authors: Isac Raluca , Basaca Diana-Georgiana , Olariu Ioana-Cristina , Stroescu Ramona F. , Ardelean Andrada-Mara , Steflea Ruxandra M. , Gafencu Mihai , Chirita-Emandi Adela , Bagiu Iulia Cristina , Horhat Florin George , Vulcanescu Dan-Dumitru , Ionescu Dan , Doros Gabriela
Published: July 2021

Background: Urinary tract infections (UTI) are common in children worldwide. Congenital anomalies of kidney and urinary tract (CAKUT) increase the risk of UTI and consequently antibiotic resistance. Antibiotic resistance represents an important public health issue worldwide. We aimed to evaluate the local trend in terms of bacterial uropathogen resistance in the western part of Romania in children with CAKUT and UTI. Methods: 252 children with CAKUT were admitted to our hospital over a five-year period. Of them, 91 developed at least one UTI episode, with a total number of 260 positive urine cultures. We collected data about age at diagnosis of CAKUT, sex, origin environment, type and side of CAKUT, number of UTIs, type of uropathogen, and uropathogens antibiotic resistance. Results: Distribution of uropathogens was Escherichia coli (38.84%), Klebsiella spp. (21.15%), Enterococcus spp. (15.76%), Proteus spp. (8.07%), Pseudomonas spp. (8.07%), Enterobacter spp. (2.3%), other Gram-negative bacteria (2.3%), and other Gram-positive bacteria (3.45%). High antibiotic resistance was detected for ampicillin, amoxicillin, and second-generation cephalosporins. Escherichia coli presented high resistance for cefepime and ceftriaxone. Pseudomonas spp. remained susceptible to amikacin, quinolones, and colistin. Vancomycin, teicoplanin, linezolid, and piperacillin/tazobactam remained effective in treating Gram-positive UTI. Conclusions: High antibiotic resistance was identified for frequently used antibiotics. Lower antibiotic resistance was observed for some broad-spectrum antibiotics. Understanding uropathogens’ antibiotic resistance is important in creating treatment recommendations, based on international guidelines, local resistance patterns, and patient particularities.
Fast-Response Liquid Crystals for 6G Optical Communications
Authors: Zou Junyu , Yang Zhiyong , Mao Chongchang , Wu Shin-Tson
Published: July 2021

We report two high birefringence and low viscosity nematic mixtures for phase-only liquid-crystal-on-silicon spatial light modulators. The measured response time (on + off) of a test cell with 2π phase change at 1550 nm, 5 V operation voltage, and 40 °C is faster than 10 ms. To improve the photostability, a distributed Bragg reflector is designed to cutoff the harmful ultraviolet and blue wavelengths. These materials are promising candidates for future 6G optical communications.