bluuebunny/crossref_metadata_2025 huggingface dataset:
Knockout of elF4E using CRISPR/Cas9 for large-scale production of resistant cucumber cultivar against WMV, ZYMV, and PRSV
Authors: Fidan Hakan , Calis Ozer , Ari Esin , Atasayar Aydin , Sarikaya Pelin , Tek Mumin Ibrahim , Izmirli Ahmet , Oz Yasemin , Firat Gulsah
Published: March 2023

CRISPR/Cas9 is one of the most robust technologies for plant breeding enabling precise and efficient modifications in a genome. This technology is being used for the manipulation of target genes in a host to develop resistance against the plant pathogens. Cucumis sativus elF4E is one of the target genes playing a key role in viral infection during interaction with potyvirus viral proteins genome linked (VPg). Nevertheless, the allelic and positional effect of elF4E mutations in C. sativus is to be clarified in elF4E-VPg interaction. In addition, there are entanglements in the massive production of pathogen-resistant cultivars suitable for commercial production using CRISPR/Cas9 technology. Therefore, we targeted different positions of the elF4E in G27 and G247 inbred lines, using specific gRNA1 and gRNA2 for the first and third exons, respectively, and 1,221 transgene-free plants were selected in segregated T1 generation, where 192 G27 and 79 G247 plants had the least mutation at Cas9 cleavage site of gRNA1 or gRNA2. Crossing was performed to see allelic effects of elfF4E mutations in F1 populations, which were homozygous and heterozygous single (elF4E_1DEL or elF4E_3DEL) and double (elF4E_1-3DEL) mutants. Disease symptoms of watermelon mosaic virus (WMV), papaya ringspot virus (PRSV), and zucchini yellow mosaic virus (ZYMV) were evaluated in both non-edited and edited F1 plants, and we did not observe any symptom in homozygous elF4E_1-3DEL and elF4E_1DEL mutants. However, homozygous elF4E_3DEL was positive in reverse transcription polymerase chain reaction (RT-PCR), even if there were no significant symptoms on the inoculated leaves. ELISA and qRT-PCR indicated lower viral accumulation in homozygous elF4E_3DEL than heterozygous and non-edited plants. Regeneration and transformation protocols were also optimized comprehensively for both the genotypes. The average number of shoots/100 explants was determined for both G27 and G247 as 13.6 and 18.0, respectively. We could not detect any distinguishing difference between the non-edited and edited F1 plants for yield and morphology. Our results demonstrate an effective route for mass production of viral resistant cultivars of cucumber to WMV, ZYMV, and PRSV. In this way, the pathogen-resistant cultivars could be generated to reduce the losses caused by these pathogens in cucumber production.
Reviewing the role of positive classroom climate in improving English as a foreign language students’ social interactions in the online classroom
Authors: Qiu Fei
Published: March 2023

The teacher and learners are cooperatively involved in the creation of a positive climate in an L2 class. In the online language learning environment today, teachers can make the best use of technology, multimedia learning, and accessibility of learners to create a supportive and effective climate. In this productive climate, the teacher and learners can have multiple forms of social interaction which can improve language learners’ communicative skills. Not only can the teacher expect better learning outcomes, but s/he can also ensure students’ wholehearted attendance in, attention to, and participation in class activities. A positive class climate and the consequent better social interactions can also enhance learners’ affective well-being. For example, higher self-esteem leads to lower levels of anxiety and better emotional health. This study aims to review the contribution of a positive classroom climate to the improvement of students’ social interaction in the online L2 classroom. To do this, the findings of the relevant studies have been presented and their implications for the construction of a positive online L2 classroom climate have been provided. Suggestions are made on how to help teachers create a positive climate in online L2 classes and how to pave the way for more effective social interactions between teachers and students and among students. Also, implications are provided for L2 teachers, researchers, and trainers, especially in the post-pandemic era.
Enter into society: Digitalized livelihoods and prosumer labor for people with disabilities in China
Authors: Bao Yaxiong , Hu Xinyu , Zhang Ruijie , Shi Xiaochuan
Published: March 2023

Background

The current debates on “digital labor” focus on a gorgeous and comprehensive experiential description and theoretical exposition but do not provide a thorough examination of the unique context and social structure. In China, the development of internet is closely tied to politics, the Chinese Government uses internet as a tool of social governance. More importantly, aside from desire-based communications produced by corporate ideology, the Chinese people’s passion for the Internet also comes from individual survival, especially the middle and lower class of information represented by the disabled people. This means that analysis of the digital labor among people with disabilities in China must be done from a variety of angles, including politics, society and culture.

Methods

This study combines life-history interviews and field research methods to explore the value and significance of digitalized livelihoods and free prosumer labor for people with disabilities in China through self-narration. Since 2020, researchers have been volunteering at two social organizations serving people with physical disabilities in Wuhan city, Hubei Province. We participated in 26 assistance activities for disabled groups which included three 14-day training camps, and interviewed 40 people with physical disabilities.

Results

This study found that although the digitalized livelihoods practice of people with disabilities is still “precarious labor” in nature, whose self-expression in the cyberspace is easy to fall into the shackles of capital flow logic. However, digital labor practice provides them with the opportunity to “sit at home, enter the community and society,” also enables them to “live independently.” More importantly, this opportunity and possibility enable people with disabilities to experience a sense of value and self-esteem as “competent people.” Therefore, in the realistic environment of structural obstacles in the social life of the people with disabilities in China, the possibility of “inclusiveness” brought by digital labor is the core value brought by digital society.
Alexinomia: The fear of using personal names
Authors: Ditye Thomas , Rodax Natalie , Welleschik Lisa
Published: March 2023

Introduction

Preliminary research based on everyday observations suggests that there are people, who experience severe fear when addressing others with their personal names. The aim of this study was to explore the extent to which this hitherto little-known psychological phenomenon really exists and to investigate its characteristic features, considering the everyday experience of not being able to use names and its impact on affected individuals and their social interactions and relationships.

Methods

In this mixed-methods study based on semi-structured interviews and psychometric testing, 13 affected female participants were interviewed and evaluated using self-report measures of social anxiety, attachment-related vulnerability, and general personality traits. An inductive content analysis and inferential statistical methods were used to analyze qualitative and quantitative data, respectively.

Results

Our findings show that affected individuals experience psychological distress and a variety of negative emotions in situations in which addressing others with their name is intended, resulting in avoidance behavior, impaired social interactions, and a reduced quality of affected relationships.

Discussion

The behavior can affect all relationships and all forms of communication and is strongly linked to social anxiety and insecure attachment. We propose calling this phenomenon Alexinomia, meaning “no words for names”.
Bibliometric analysis of tuberculosis molecular epidemiology based on CiteSpace
Authors: Zheng Mei-qin , Li Xi-xi , Xu Rui , Liu Shuo , Rui Zhi-yong , Guo Zhen-yong , Chen Di
Published: March 2023

Background

Tuberculosis is a communicable disease that is a major cause of ill health. Bibliometrics is an important statistical methodology used to analyze articles and other publications in the literature study. In this study, publications on molecular epidemiology were analyzed using bibliometric analysis. The statistical analysis of influential publications, journals, countries and authors was first conducted.

Methods

The Web of Science database was searched for publications on the molecular epidemiology of tuberculosis with the keywords “tuberculosis” and “molecular epidemiology” in the title. The number of publications, citation analysis, co-authorship of the author, institution and country, keyword co-occurrence, and reference co-citations were analyzed.

Results

A total of 225 journal articles were retrieved. The mean citation was 37.72 per article and 292.69 per year. The annual publications on molecular epidemiology fluctuated within a certain range in the past. Journal of Clinical Microbiology is the most published journal with 33 articles. RASTOGI N is the most prolific author with 11 articles. The top 1 research institution is Inst Pasteur Guadeloupe. Stratified by the number of publications, the USA was the most prolific country. It also cooperates closely with other countries. Burstness analysis of references and keywords showed that the developing research trends in this field mainly focused on “genetic diversity” and “lineage” during the past decade.

Conclusion

The annual publications on tuberculosis molecular epidemiology fluctuated within a specific range in the past decade. The USA continues to dominate research output and funding. The exchange of expertise, ideas, and technology is of paramount importance in this field. More frequent and deeper cooperation among countries or institutions will be essential in the future.
Association between multimorbidity and memory-related diseases among middle-aged and older adults: Evidence from the China Health and Retirement Longitudinal Study
Authors: Chen Chen , Zhao Yihao , Su Binbin , Wu Yu , Zhong Panliang , Zheng Xiaoying
Published: March 2023

Objectives

This study aimed to examine the cross-sectional and longitudinal association between multimorbidity and memory-related diseases (MDs) among Chinese middle-aged and older adults.

Methods

This study included 8,338 subjects who participated in the China Health and Retirement Longitudinal Study (CHARLS). Logistic regression and Cox proportional hazards regression models were used to explore the association and effect of multimorbidity on MDs.

Results

The overall prevalence of MDs was 2.52%, and the mean multimorbidity number was 1.87. In a cross-sectional analysis, compared with the no multimorbidity group, groups of multimorbidity with four or more non-communicable diseases (NCDs) were more likely to have MDs (OR: 6.49, 95%CI: 4.35–9.68). Within 2.7 years of follow-up, 82 cases of MDs (1.12%) were reported, and participants with multimorbidity were more likely to have new-onset MDs than participants without multimorbidity (HR: 2.93, 95%CI: 1.74–4.96).

Conclusion

Multimorbidity is associated with MDs among Chinese middle-aged and older adults. This relationship gradually strengthens with the severity of multimorbidity, which indicates that early prevention for people with multimorbidity may reduce the risk of MDs.
A “willingness to be orchestrated”: Why are UK diplomats working with tobacco companies?
Authors: Alebshehy Raouf , Silver Karin , Chamberlain Phil
Published: March 2023

Background

The tobacco epidemic is global and addressing it requires global collaboration. International and national policies have been adopted to promote collaboration for tobacco control, including an obligation on diplomatic missions to protect public health from the vested interests of the tobacco industry. However, incidents of diplomats engaging with the tobacco industry are still occurring despite these regulations. This paper presents a case study of a British ambassador actions, and it points to some of the challenges researchers face in monitoring such incidents.

Methods

The incident studied in this paper was first identified through regular media monitoring conducted by the Tobacco Control Research Group at the University of Bath. The incident was further investigated by using the tools made available by the United Kingdom (UK) Freedom of Information Act, including submitting a request, asking for internal review, and submitting a complaint to the Information Commissioner's Office.

Results

We identified clear evidence of the UK ambassador to Yemen opening a cigarette factory, part owned by British American Tobacco (BAT), in Jordan. Our investigation revealed a lack of documentation of this and similar incidents of interaction between diplomats and the tobacco industry. We raise concerns about the actions of diplomats which contravene both national and international policies.

Discussion

Monitoring and reporting such activities produces several challenges. Diplomats' interactions with the tobacco industry represent a major concern for public health as such interactions seem to be systematically repeated. This paper calls for action to better implement national and international policies to protect the public health including in low- and middle-income countries (LMICs).
Error driven synapse augmented neurogenesis
Authors: Perrett Adam , Furber Steve B. , Rhodes Oliver
Published: March 2023

Capturing the learning capabilities of the brain has the potential to revolutionize artificial intelligence. Humans display an impressive ability to acquire knowledge on the fly and immediately store it in a usable format. Parametric models of learning, such as gradient descent, focus on capturing the statistical properties of a data set. Information is precipitated into a network through repeated updates of connection weights in the direction gradients dictate will lead to less error. This work presents the EDN (Error Driven Neurogenesis) algorithm which explores how neurogenesis coupled with non-linear synaptic activations enables a biologically plausible mechanism to immediately store data in a one-shot, online fashion and readily apply it to a task without the need for parameter updates. Regression (auto-mpg) test error was reduced more than 135 times faster and converged to an error around three times smaller compared to gradient descent using ADAM optimization. EDN also reached the same level of performance in wine cultivar classification 25 times faster than gradient descent and twice as fast when applied to MNIST and the inverted pendulum (reinforcement learning).
Predicting outcomes of hematological malignancy patients admitted to critical care
Authors: Tridente Ascanio , Dempsey Nina C. , Khalifa Mai , Goddard Jack , Shuker Katy , Hall Joni , Sorour Youssef , Wright Josh , Webber Stephen , Mills Gary H. , Snowden John A.
Published: March 2023

Background

Critical care (CC) admission has traditionally been viewed as likely to result in a poor outcome for hematological malignancy (HM) patients. Such a view can have implications for decisions surrounding CC admission. Recent studies have challenged this poor prognostication, however, there still remains limited data to support CC admission and escalation decisions and to elucidate risk factors which independently predict short- and longer-term survival outcomes.

Methods

We retrospectively analyzed a large cohort of adult HM patients (n=437) admitted to CC over a sixteen-year period, with the specific aim of identifying risk factors present at CC unit admission that could help to predict outcome. We assessed all-cause mortality at CC discharge (CC mortality, primary outcome) and at further time points (hospital discharge and 12-months post-discharge from CC). Single variable and multivariate analyses were performed to identify independent predictors of outcome.

Results

CC unit and hospital mortality rates were 33.4% (146 patients) and 46.2% (202 patients) respectively. At six-month and one-year follow-up, mortality increased to 59.5% and 67.9% respectively. At single variable adjusted regression analysis, eight factors were associated with CC mortality: APACHE II score, the number of organs supported, requirement for continuous renal replacement therapy (CRRT), cardiovascular support, or respiratory support (invasive and non-invasive), the ratio between arterial partial pressure of oxygen (PaO2) and the inspired oxygen concentration (FiO2) (P/F ratio) on CC admission, and the lowest P/F ratio during CC admission. However, only three factors showed independent predictive capacity for CC outcome at multivariate logistic regression analysis; APACHE II score on admission, requirement for ventilation and lowest P/F ratio.

Conclusion

One third of HM patients admitted to CC died on the unit and, following admission to CC, approximately one-third of HM patients survived over 1 year. Our data show that, while a diagnosis of HM should not preclude admission of patients who might otherwise benefit from CC support, the prognosis of those with a high APACHE II score upon admission, or those requiring IMV remains poor, despite considerable advances in IMV techniques.
Stabilization of water-in-water emulsion upon solvent shifting
Authors: Céré Claire , Béven Laure , Douliez Jean-Paul
Published: March 2023

We report the stabilization of all-aqueous droplets upon addition of ethanol, which were further transformed into robust capsules. An all-aqueous emulsion consisting of dextran (Dex)-rich droplets in a polyethylene glycol (PEG)-rich continuous phase was used as templates. Addition of ethanol induced gelation of dextran, forming aggregated pellets of poor interest. However, this feature was prevented by initially adding bovine serum albumin (BSA) or positively charged silica particles so that in this case, stable droplets formed upon solvent addition, which no longer coalesced with time. An alternative method consisting of pouring concentrated Dex solution in a mixture of PEG, ethanol and BSA or particles, also afforded stable droplets. These stable droplets were further converted into robust capsules, using carbodiimide chemistry or silylation. We expect this method for preparing capsules to be of interest for various applications in the field of microencapsulation.
“Oh, the places you'll go!”—But not for those children trapped by family violence
Authors: Zentveld Elisa
Published: March 2023

Tourism is often regarded as an opportunity to “get away” and “escape;” a time for rest and relaxation and “getting away from it all.” Traveling to new places is also thought to be an important “classroom” for children. Traveling to new places was a theme in books by the famous children's author and illustrator Theodor Seuss Geisel (who published under the name “Dr. Seuss”). Whilst Dr. Seuss had numerous hardships in his life including a prolonged illness, no children, and his first wife's suicide, he wrote books that were considered uplifting and fun to read. In the Dr. Seuss book “Oh, the places you'll go!” he wrote “you're off to great places! Today is your day! Your mountain is waiting, so….get on your way!” The idea that children should feel free to visit great places, experience life to its fullest, and feel safe seems on the surface to be axiomatic. Sadly, this is not the experience for all children. Taking a whole tourism systems approach, this paper outlines how family violence impacts each of the five elements in the tourism system. Whilst tourism studies often focus on the positive and boosterish side, to fully understand children in tourism, it is necessary to look at it in its entirety. This means acknowledging and understanding how children's tourism experiences might be limited. This paper reveals that children can be denied permission to undertake certain travel due to court orders or denial by the perpetrator of family violence. It is hoped that greater awareness of this topic may result in better and fairer family law orders to allow more tourism experiences for children who live with or have lived with family violence.
Drain vs. no-drain for acetabular fractures after treatment via a modified stoppa approach: A retrospective study
Authors: Jin Lin , Wang Zhongzheng , Zhao Kuo , Lian Xiaodong , Chen Wei , Zhang Yingze , Hou Zhiyong
Published: March 2023

Objective

The aim of this study was to compare the clinical efficacy of close suction drainage (CSD) and no-CSD after a modified Stoppa approach for the surgical fixation of acetabular fractures.

Methods

This retrospective study included 49 consecutive acetabular fracture patients, who presented to a single level I trauma center for surgical fixation, using a modified Stoppa approach from January 2018 to January 2021. All surgeries were performed by a senior surgeon using the same approach, and the patients were divided into two groups based on whether CSD was used after the operation. Details of the patient demographics, fracture characteristics, intraoperative indicators, reduction quality, intra and postoperative blood transfusion, clinical outcomes, and incision-related complications were collected.

Results

No significant differences were found in the demographics, fracture characteristics, intraoperative indicators, reduction quality, clinical outcomes, and incision-related complications between the two groups (P > 0.05). The use of CSD was associated with a significantly higher postoperative blood transfusion volume (P = 0.034) and postoperative blood transfusion rate (P = 0.027). In addition, there was a significant difference in postoperative temperatures, especially on postoperative Day 2 (no-CSD 36.97 ± 0.51°C vs. CSD 37.34 ± 0.69°C, P = 0.035), and higher visual analogue scale (VAS) scores, especially on postoperative Day 1 (no-CSD 3.00 ± 0.93 vs. CSD 4.14 ± 1.43, P = 0.002) and 3 (no-CSD 1.73 ± 0.94 vs. CSD 2.48 ± 1.08, P = 0.013).

Conclusion

The results of this study suggest that routine use of CSD should not be recommended for patients with acetabular fractures after surgical fixation using a modified Stoppa approach.
Spatio-temporal and trade export risk analysis of bluetongue disease in France: A case study of China
Authors: Yang Qiao-ling , Zhang Shu-wen , Qiu Song-yin , Zhang Qiang , Chen Qin , Niu Bing
Published: March 2023

Bluetongue disease (BT) is a viral disease that can be introduced through imported animals and animal products, affecting local animal husbandry. In this study, the spatial and temporal patterns of BT outbreaks (outbreak: a BT infection in cattle, sheep, or goats on a farm, involving at least one infected animal) in France were analyzed and the risk of introducing bluetongue virus (BTV) into countries through trade was assessed. A spatiotemporal analysis of BT reported during the study period (2015–2018) showed that there were clustered outbreaks of BT in France in 2016 and 2017, with outbreaks concentrated from August to December. The outbreak moved eastward from the center of mainland France to surrounding countries. A semi-quantitative risk analysis framework was established by combining the likelihood assessment and consequence analysis of introducing BTV into trading countries through trade. Exemplified by China, the research showed that in the analysis of the likelihood of BTV from France being introduced into trading countries through live cattle trade, China imports a large number of live cattle, bringing high risks. The likelihood of introducing bovine semen into trading countries was similar to that of live cattle, but the harm caused by the trade in live cattle was higher than that caused by the trade in bovine semen. This risk analysis framework can provide a reference for other countries to quickly assess the risk of bluetongue transmission in import and export trade.
Cryopreservation of African painted dog (Lycaon pictus) ovarian tissue
Authors: Hartzler Kate E. , McCartney Chiara , Songsasen Nucharin , Nagashima Jennifer B.
Published: March 2023

Development of techniques for the preservation and use of gonadal tissues are increasingly needed for the genetic management of the endangered African painted dog (Lycaon pictus). Here we evaluated two cryopreservation techniques for ovarian tissue (2 × 2 × 1 mm3 fragments, n = 11 individuals): needle immersed vitrification (NIV), with equilibration in a 7.5% dimethyl sulfoxide (DMSO) and 7.5% ethylene glycol (EG) solution, and vitrification in a 15% DMSO, 15% EG, and 0.5 M sucrose solution, and slow freezing in cryovials with either the equilibration (SF-E) or vitrification (SF-V) solutions. Following warming, tissues were either fixed and embedded for evaluation of density of morphologically normal follicles, semi-quantitative scoring of stromal cell preservation, and apoptotic index (TUNEL stain), and/or flash-frozen for expression of proliferation (PCNA), apoptosis (CASP3, BCL2), or oxidative stress (GPX3, SOD1, SOD2) pathway genes (n = 4). Needle immersed vitrification maintained higher density of morphologically normal follicles compared to the slow freezing protocols applied (p < 0.05), with no significant changes in expression of select genes among treatment groups. A slight increase in apoptotic index was observed in all cryopreservation groups, but only reached significance in SF-E compared with fresh tissue controls (p < 0.05). Future research should be dedicated to developing improved methods for ovarian tissue culture in the species, both as a means to evaluate the efficacy of tissue cryopreservation techniques and for the production of viable oocytes from banked ovarian tissue in the endangered African painted dog.
A Practical Deceleration Control Method, Prototype Implementation and Test Verification for Rail Vehicles
Authors: Ma Tianhe , Tian Chun , Wu Mengling , Zhou Jiajun , Liu Yinhu
Published: March 2023

Currently, the theoretical braking force control mode, characterized by actual deceleration as an unstable open-loop output, is the most widely used brake control mode in trains. To overcome the shortcomings of non-deceleration control modes, a deceleration control mode is proposed to realize the closed-loop control of train deceleration. First, a deceleration control algorithm based on parameter estimation was derived. Then, the deceleration control software logic was designed based on the existing braking system to meet the engineering requirements. Finally, the deceleration control algorithm was verified through a ground combination test bench with real brake control equipment and pneumatic brakes. The test results show that the deceleration control can make the actual braking deceleration of the train accurately track the target deceleration in the presence of disturbances, such as uncertain brake pad friction coefficients, line ramps, vehicle loads and braking force feedback errors, as well as their combined effects, and does not affect the original performance of the braking system. The average deceleration in the deceleration control mode is relatively stable, and the control error of instantaneous deceleration is smaller.
A Multivariable Method for Calculating Failure Probability of Aeroengine Rotor Disk
Authors: Li Guo , Liu Junbo , Yang Liu , Zhou Huimin , Ding Shuiting
Published: March 2023

The probabilistic damage tolerance analysis of aeroengine rotor disks is essential for determining if the disk is safe. To calculate the probability of failure, the numerical integration method is efficient if the integral formula of the probability density function is known. However, obtaining an accurate integral formula for aeroengine disks is generally complicated due to their complex failure mechanism. This article proposes a multivariable numerical integral method for calculating the probability of failure. Three random variables (initial defect length a, life scatter factor S, and stress scatter factor B) are considered. A compressor disk model is evaluated. The convergence, efficiency, and accuracy of the proposed method are compared with the Monte Carlo simulation and importance sampling method. The results show that the integral-based method is 100 times more efficient under the same convergence and accuracy conditions.
Impact of PGPR Formulations Combined with Exogenous IBA Levels to Enhance Root Capacity in Poinsettia Cuttings
Authors: Parlakova Karagöz Fazilet
Published: March 2023

The commercial production of poinsettias begins with the propagation of apical cuttings from stock plants. The aim of the study was to use bacterial mixtures prepared with PGPRs in the cultivation of stock mother poinsettia plants and determine an effective IBA dose to increase root development and root yield of cuttings prepared from these stock mother plants. Rooted cuttings of Euphorbia pulcherrima Willd.ex Klotzsch were used for planting stocks of mother plants. Eight different bacterial isolates were mixed in triple combinations to form 4 different bacterial formulations: (BI) formulation 1 (Paenibacillus polymyxa TV-12E + Pseudomonas putida TV-42A + Pantoea agglomerans RK-79); (BII) formulation 2 (Bacillus megaterium TV-91C + Pantoea agglomerans RK-92 + Bacillus subtilis TV-17C); (BIII) formulation 3 (Bacillus megaterium TV-91C + Pantoea agglomerans RK-92 + Kluyvera cryocrescens TV-113C); and (BIV) formulation 4 (Bacillus megaterium TV-91C + Pantoea agglomerans RK-79 + Bacillus megaterium TV-6D). In the first year, rooted cuttings of stock mother plants were dipped in liquid microbial solution (bacterial formulations) for 15 min and then planted in plastic pots. In the second year, the same bacterial formulations were freshly prepared and applied to the 1-year-old stock mother plants as irrigation water for the second time. Amounts of 0 (control), 1000, 1500, and 2000 mg L−1 IBA doses were used for rooting cuttings taken from the 2-year-old stock mother plants. In the experimental group treated with the BI bacterial formulation, the number of rooted cuttings (NR) increased by 14.26% and 19.00%, compared with the control, in response to IBA 1500 mg L−1 and IBA 2000 mg L−1 treatment, respectively. Compared to the mean root length of the cuttings in the control treatment, the mean root length of the cuttings in the BIV treatment increased by 18.83%. The mean root length values decreased with the increase in IBA doses. The highest value of the number of mean shoots (NAS) was in cuttings treated with BI + 1500 treatment, which was 1.93 times higher than the control. The highest nitrogen content (5.73%) was determined in the bract leaf samples of the BIV application, and an 81.33% nitrogen increase was detected in the related application when compared to the control. In terms of P, Ca, and Fe contents, higher values were obtained from the BIV application when compared to the control application. The highest values of K content were determined in BIII and BII applications. This study provides positive effects on the feedback of stock mother plants with PGPR to provide sprout production by cuttings technique. It has been revealed that lower IBA dose applications can be recommended for rooting cuttings taken from mother plants treated with the BIV bacterial formulation.
Effect of Biostimulants on Leafy Vegetables (Baby Leaf Lettuce and Batavia Lettuce) Exposed to Abiotic or Biotic Stress under Two Different Growing Systems
Authors: Clément Justine , Delisle-Houde Maxime , Nguyen Thi Thuy An , Dorais Martine , Tweddell Russell J.
Published: March 2023

Plant biostimulants are substances or microorganisms aimed at promoting plant growth by increasing the mineral nutrition efficiency, tolerance to environmental stress, and crop quality traits. This new category of crop inputs has been capturing the interest of both researchers and agriculture takeholders in light of the promising effects they could have on crop productivity and sustainability. This study investigated a variety of biostimulants for their effect on germination rates, plant health, chlorophyll fluorescence parameters, SPAD index, and growth of baby leaf lettuce and Batavia lettuce submitted to biotic (absence/presence of Pythium ultimum in the growing medium) or abiotic (0, 40, 80, and 120 mM NaCl L−1 concentrations and −0.5, −2, −4, and −6 kPa water potentials) stresses when grown in a greenhouse under conventional and organic cultivation. The results obtained show that lettuce response to biostimulants was influenced by the type or level of stress applied and the growing system used. The effects of the tested biostimulants varied from strongly detrimental to strongly beneficial.
Agronomic Practices Alter Regulated Effects of Easily Extractable Glomalin-Related Soil Protein on Fruit Quality and Soil Properties of Satsuma Mandarin
Authors: Lei An-Qi , Yang Qing-Hua , Zhang Ying , Liao Wen-Yue , Xie Ya-Chao , Srivastava Anoop Kumar , Hashem Abeer , Alqahtani Mashael Daghash , Abd_Allah Elsayed Fathi , Wu Qiang-Sheng , Zhang Yi
Published: March 2023

Easily extractable glomalin-related soil protein (EEG) released from arbuscular mycorrhizal fungi exhibits many roles in soil and plants, while it is not clear whether the biostimulator alone or in combination with agronomic practices can improve soil characteristics and fruit quality in citrus. The objective of this study was to analyze the effects of foliar sprays of EEG, singly or in combination with fruit bagging (FB), reflective film mulching (RF), and grass-proof cloth mulching (GPC) on root mycorrhizal colonization rate, fruit internal and external quality, and soil structure and fertility on an early-ripening Satsuma mandarin “Oita 4” (Citrus unshiu cv. Oita 4). Exogenous EEG application increased root mycorrhizal colonization, while agronomic practices dramatically inhibited root mycorrhizal colonization in EEG-treated trees. Foliar EEG application significantly improved the internal and external quality of fruits, but the combination of three agronomic practices with EEG did not further enlarge the improved effect on the external quality of fruit and even produced an inhibitory effect. Also, the addition of RF further amplified the improved effect of EEG on the contents of fruit vitamin C and soluble solids. EEG application also significantly elevated sucrose in the fruit pith and juice sac, fructose in the fruit peel, fruit pith, segment membrane, and juice sac, and glucose in the fruit pith, segment membrane, and juice sac. Additional RF treatment could increase sucrose in the fruit peel and juice sac as well as glucose in the fruit peel to varying degrees in EEG-treated trees. EEG application also significantly increased soil organic carbon, mean weight diameter, nitrate nitrogen, ammonium nitrogen, available phosphorus, and available potassium levels, with additional agronomic practices almost reducing the promoting effect of EEG on these soil variables. It has been summarized that a single EEG application had positive effects on fruit quality and soil fertility, while the additional agronomic practices resulted in little change or even suppressed the positive effects of EEG.
Differences in Soil Microbiota of Continuous Cultivation of Ganoderma leucocontextum
Authors: Yao Chunxin , Tao Nan , Liu Jiaxun , Liang Mingtai , Wang Hui , Tian Guoting
Published: March 2023

The tendency of microbiota changes in the soil of Ganoderma leucocontextum continuous cultivation is investigated by high-throughput sequencing technology. Medium bag cultivation of G. leucocontextum with uncultivated soil significantly increased the organic matter (OM), hydrolyzable nitrogen, available phosphorus, and available potassium content of soil. The relative abundance of the dominant beneficial bacteria (Sphingomonas spp., Mucilaginibacter spp., Bryobacter spp., and Bradyrhizobium spp.) for G. leucocontextum continuous cultivation, was decreased in the soil. Mortierella spp. and Pyrenochaeta spp. were the dominant fungi with negative effects on G. leucocontextum cultivation in the soil. The correlations between the microbiota and soil physicochemical properties indicated that continuous cultivation not only caused changes in the soil physicochemical factors but also affected the structure of dominant microbial communities, especially bacteria and environmental factors.
National Dog Survey: Describing UK Dog and Ownership Demographics
Authors: Anderson Katharine L. , Casey Rachel A. , Cooper Ben , Upjohn Melissa M. , Christley Robert M.
Published: March 2023

With dogs being the most commonly owned companion animal in the United Kingdom, knowledge about dog demographics is important in understanding the impact of dogs on society. Furthermore, understanding the demography of dog owners is also important to better target support to dogs and their owners to achieve optimal welfare in the canine population. Combining natural fluctuations in the population and unprecedented events such as the COVID-19 pandemic, the need for an up-to-date large-scale dataset is even more paramount. In order to address this, Dogs Trust launched the ‘National Dog Survey’ to provide a large population-level dataset that will help identify key areas of concern and needs of owners and their dogs. The online survey was completed by a total of 354,046 respondents owning dogs in the UK, providing data for 440,423 dogs. The results of this study highlight dog demographics, including acquisition and veterinary factors, as well as owner demographic and household information. Finally, general trends in ownership, and more specifically those following the COVID-19 pandemic, are described. This paper’s findings provide valuable insight into the current population of dogs and their owners in the UK, allowing for the most appropriate products, services, interventions and regulations to be developed, reducing the likelihood of negative welfare outcomes such as health and behaviour issues, relinquishment or euthanasia. Furthermore, with significant changes to the dog population following the COVID-19 pandemic highlighted, this dataset serves as an up-to-date baseline for future study comparisons to continue to monitor trends and patterns of the dog population and dog owners going forwards.
Optimizing Bull Semen Cryopreservation Media Using Multivariate Statistics Approaches
Authors: Mokhtassi-Bidgoli Ali , Sharafi Mohsen , Benson James D.
Published: March 2023

Cryo-injury reduces post-thaw semen quality. Extender components play a protective role, but existing experimental approaches do not elucidate interactions among extender components, semen samples, and post-thaw quality. To identify optimal concentrations for 12 extender ingredients, we ran 122 experiments with an I-optimal completely random design using a large dataset from our previous study. We obtained a maximum predicted total motility of 70.56% from an I-optimal design and 73.75% from a Monte Carlo simulation. Individual bull variations were significant and interacted with extenders independently. 67% of bulls reliably preferred extender formulations to reach maximum motility. Multifactor analysis suggests that some antioxidants may offer superior protection over others. Partial least squares path modeling (PLS-PM) found the highest positive loadings for glutathione in the antioxidant class, glycerol in the CPA class, and fructose in the basic compounds class. The optimal ranges for milk, water, and ethylene glycol were extremely narrow. Egg yolk, cholesterol-loaded cyclodextrin, and nerve growth factor had medium-loading impacts. PLS-PM showed that CPA, osmoregulators, and basic components were the most efficient contributors to motility, while the antioxidant and extracellular protectant classes had less efficiency. Thus, ingredients, concentrations, and interactions of extender compounds are critical to extender formulation, especially when using multiple compounds with the same function.
Effect of Fermented Rapeseed Meal in Feeds for Growing Piglets on Bone Morphological Traits, Mechanical Properties, and Bone Metabolism
Authors: Muszyński Siemowit , Dajnowska Aleksandra , Arciszewski Marcin B. , Rudyk Halyna , Śliwa Jadwiga , Krakowiak Dominika , Piech Małgorzata , Nowakowicz-Dębek Bożena , Czech Anna
Published: March 2023

Quality feed is essential for correct bone development and proper functioning of animals. Post-weaned piglets experience a radical change in eating behaviour that can influence their feed intake. For this reason, functional feed additives and ingredients that can be used in post-weaning feeds are needed. The objective of this study was to evaluate the effects of partially replacing wheat with rapeseed meal fermented using Bacillus subtilis strain 87Y on overall bone quality and bone metabolism in weaner piglets. From the 28th day of life, barrows were fed either a standard wheat-based diet or a diet containing 8% fermented rapeseed meal (FRSM) with or without a feed additive containing enzymes, antioxidants, probiotics, and prebiotics. The experimental period lasted 60 days, after which femur quality indices were assessed. Differences in bone length and weight were observed, but there were no changes in bone mineralization or bone mid-diaphysis morphometrical traits between treatments. FRSM inclusion reduced bone mid-diaphysis biomechanical properties, but these changes were dependent on feed-additive supplementation. Analysis of the levels of serum bone turnover markers suggests the intensification of bone resorption in FRSM-fed groups as deoxypyridinoline levels increase. The results obtained warrant further research on what the disturbances in bone mechanical properties and metabolism observed in FRSM-fed weaners means for the subsequent fattening period.
Effects of Core Antigen Bacterin with an Immunostimulant on Piglet Health and Performance Outcomes When Challenged with Enteric and Respiratory Pathogens
Authors: Cull Charley A. , Singu Vijay K. , Bromm Jenna J. , Lechtenberg Kelly F. , Amachawadi Raghavendra G. , Cull Brooke J.
Published: March 2023

A total of 90 pigs, approximately one day of age, were used in a 42-day study to evaluate whether Endovac-Porci, a core antigen vaccine with an immunostimulant, provides piglets with broad-spectrum protection against the enteric and respiratory effects of Gram-negative bacteria. This study was a single-site, randomized, prospective, blinded, comparative placebo-controlled design. Individual pigs were randomly allocated to 1 of 2 treatments in a randomized design. An individual pig was considered the experimental unit for the farrowing phase (Study day 0 to 21), and the pen was considered the experimental unit for the nursery phase (Study day 21 to 42). Thus, there were 45 replications per treatment during the farrowing phase and 15 replications per treatment during the nursery phase. Treatments included a control product (saline; CP) and an investigational product (Endovac-Porci; IVP). On Study day 23, all pigs were challenged with enterotoxigenic Escherichia coli strain expressing K88 (F4) fimbriae and Pasteurella multocida. Individual pigs were weighed and feed consumption was measured to determine body weight gain, average daily gain, and feed-to-gain ratio. Clinical and fecal scores and overall health were recorded daily. Overall, administering the IVP to pigs led to an increase (p < 0.01) in body weight gain and average daily gain compared to pigs administered the CP. Pigs administered the IVP had reduced (p < 0.01) mortality compared to pigs administered the CP. There was a Study day × treatment interaction on clinical and fecal scores (p < 0.01). There was also a main effect of Study day where clinical and fecal scores increased (p < 0.01) as the Study day increased. Treatment also had an effect on clinical and fecal scores, where pigs administered the IVP had lower (p < 0.01) clinical and fecal scores compared to pigs administered the CP. In conclusion, administering pigs with the Endovac-Porci vaccination significantly improved the performance (i.e., body weight, body weight gain, and average daily gain) and health (i.e., clinical and fecal scores), while reducing the overall mortality in pigs challenged with E. coli K88 orally and Pasteurella multocida intranasally post-weaning. Results from this study suggest that Endovac-Porci could provide broad-spectrum protection against enteric and respiratory effects of Gram-negative bacteria in piglets.
Antimicrobial Defined Daily Dose in Neonatal Population: Validation in the Clinical Practice
Authors: Villanueva-Bueno Cristina , Montecatine-Alonso Elena , Jiménez-Parrilla Francisco , González-López María , Manrique-Rodríguez Silvia , Moreno-Ramos Francisco , Cañete-Ramírez Carme , Dolz Elisenda , García-Robles Ana , Caro-Teller José Manuel , Moral-Pumarega María Teresa , Bergon-Sendin Elena , Gómez-Trevecedo Calvo María Teresa , Gallego-Fernández Carmen , Vayo-Benito Concepción Álvarez del , Mejías-Trueba Marta , Gil-Navarro María Victoria ,   Paediatric Antimicrobial Defined Daily Dose Study Group (KiDDDs)
Published: March 2023

Background: Currently, there is no validated method for estimating antimicrobial consumption in the neonatal population, as it exists for adults using Defined Daily Doses (DDD). In neonatology, although there are different methods, each one with advantages and disadvantages, there is no unified criterion for use. The aim of this study is to validate the neonatal DDD designed as a new standardised form of antimicrobial consumption over this population. Methods: The validation of the neonatal DDD, Phase II of the research project, was carried out through a descriptive observational study. Periodic cut-offs were performed to collect antimicrobial prescriptions of neonates admitted to the neonatology and intensive care units of nine Spanish hospitals. The data collected included demographic variables (gestational age, postnatal age, weight and sex), antimicrobial dose, frequency and route of administration. The selection of the optimal DDD value takes into account power value, magnitude obtained from the differences in the DDD, statistical significance obtained by the Wilcoxon test and degree of agreement in the stipulated doses. Results: Set of 904 prescriptions were collected and finally 860 were analysed based on the established criteria. The antimicrobials were mostly prescribed in the intensive care unit (63.1%). 32 different antimicrobials were collected, and intravenous administration was the most commonly used route. Neonatal DDD were defined for 11 different antimicrobials. A potency > 80% was obtained in 7 antibiotics. The 57.1% of the selected DDD correspond to phase I and 21.4% from phase II. Conclusion: DDD validation has been achieved for the majority of intravenously administered antimicrobials used in clinical practice in the neonatal population. This will make it possible to have an indicator that will be used globally to estimate the consumption of antimicrobials in this population, thus confirming its usefulness and applicability.
Motion Control of Autonomous Underwater Helicopter Based on Linear Active Disturbance Rejection Control with Tracking Differentiator
Authors: Li Haoda , An Xinyu , Feng Rendong , Chen Ying
Published: March 2023

As a new disk-shaped autonomous underwater vehicle (AUV), the autonomous underwater helicopter (AUH) is devoted to subsea operations, usually diving into the seabed and docking with a subsea docking system. Due to the motion control’s performance, the AUH’s stability and steady-state accuracy are affected remarkably while docking. Moreover, considering the difficulties of hydrodynamic modeling of AUHs, the classical model-based control method is unsuitable for AUHs. Moreover, there is a large gap between the hydrodynamic simulation results and real situations. Hence, based on the data-driven principle, the linear active disturbance rejection control with a tracking differentiator (LADRC-TD) algorithm is employed for AUH depths and heading control. As the simulation experiments prove, LADRC and LADRC-TD have better anti-interference performance when compared with PID. According to the pool experiments, overshoots of the LADRC-TD are 20 cm and 3° for the depth control and heading control, respectively, which are superior to PID and LADRC. Meanwhile, the steady-state accuracy of the LADRC-TD is ±21 cm and ±2.5° for the depth and heading control, respectively, which is inferior to PID and the same as LADRC.