bluuebunny/crossref_metadata_2025 huggingface dataset:
Status Identification in Support of Fishing Effort Estimation for Tuna Longliners in Waters near the Marshall Islands Based on AIS Data
Authors: Lu Zhengwei , Song Liming , Jiang Keji
Published: February 2024

Visualising the fishing behaviour of vessels and quantifying the spatial distribution of fishing effort is the scientific basis for assessing and managing fisheries resources. The information on the dynamics of fishing vessel voyages provided by the automatic identification system (AIS) of vessels serves as high-precision fishery data and provides a means of quantifying fishing effort with high spatial and temporal resolution in the tuna longline fishery. Based on the AIS data of five tuna longliners operating in the waters near the Marshall Islands from 2020 to 2021, this study used three methods, namely the threshold screening method, the construction of a BP neural network and the support vector machine (SVM) to identify the fishing and non-fishing status of the tuna longliners, respectively. This study investigates the status identification and fishing effort estimation of the tuna longliner (VESSEL A) in 2021 based on the constructed optimal model, and spatial correlation analyses are performed between the fishing effort estimated in hours based on AIS data and in hooks based on fishing logbook data, by month. The results showed (1) the recognition accuracy of the threshold screening method is 89.9%, the recognition accuracy of the BP neural network classification model is 95.11%, the kappa coefficient is 0.51, the recognition accuracy of the SVM classification model is 95.74% and the kappa coefficient is 0.52; (2) in comparison, the SVM classification model performs better than the other two status identification methods for tuna longliners; and (3) the correlation coefficients between the two types of effort of VESSEL A were greater than 0.79 on all fishing months, indicating that there was no significant difference in the spatial and temporal distribution between the two types of effort. This study suggests that the SVM model can be used to identify the status and estimate the fishing effort of longliners.
Vortex-Breakdown Efficiency of Planar Regular Grid Structures—Towards the Development of Design Guidelines
Authors: Sirois Julien , Sanjosé Marlène , Sanchez Fabian , Brailovski Vladimir
Published: February 2024

The work presented here aims to provide design guidelines to create vortex-damping structures. A design of experiment was developed to investigate the individual and combined effects of the geometrical properties of planar regular grid structures, i.e., the wire diameter, the porosity, and the inter-grid spacing, on their vortex-breakdown performance. The simulations were carried out using a commercial unsteady RANS solver. The model relies on the Von Karman street effect to generate vortices in a pipe which are convected downstream, where they interact with an array of grids. The vortex-breakdown efficiency is characterized by the pressure drop, the residual turbulent kinetic energy, the flow homogeneity, and the size of the transmitted vortices. The wire diameter is shown to be an important design lever as it affects the level of distortion of the transmitted vortices. Increasing the number of grids augments the pressure loss, but their contribution to vortex breakdown is otherwise limited when the wire diameter is small. The influence of grid spacing strongly depends on the wire diameter and grid alignment. For instance, minimizing this gap reduces the pressure drop for the inline configurations, but increases the pressure drop for the offset configurations.
Curcumin Changed the Number, Particle Size, and miRNA Profile of Serum Exosomes in Roman Laying Hens under Heat Stress
Authors: Kang Kai , Gao Wen , Cui Yanfeng , Xiao Mei , An Lilong , Wu Jiang
Published: February 2024

Exosomes have the ability to transport RNA/miRNAs and possess immune modulatory functions. Heat stress, a significant limiting factor in the poultry industry, can induce oxidative stress and suppress the immune responses of laying hens. In this study, we investigated the expression profiles of serum exosomes and their miRNAs in Roman laying hens who were fed a diet with either 0 or 200 mg/kg curcumin under heat stress conditions. The numbers of exosomes were significantly higher in both the HC (heat stress) and HT (heat stress with 200 mg/kg curcumin) groups compared to the NC (control) group and NT (control with 200 mg/kg curcumin) group (p < 0.05). Additionally, we observed that the most prevalent particle diameters were 68.75 nm, 68.25 nm, 54.25 nm, and 60.25 nm in the NC, NT, HC, and HT groups, respectively. From our sRNA library analysis, we identified a total of 863 unique miRNAs; among them, we screened out for subsequent bioinformatics analysis a total of 328 gga-miRNAs(chicken miRNA from the miRbase database). The KEGG pathways that are associated with target genes which are regulated by differentially expressed miRNAs across all four groups at a p-value < 0.01 included oxidative phosphorylation, protein export, cysteine and methionine metabolism, fatty acid degradation, ubiquitin-mediated proteolysis, and cardiac muscle contraction. The above findings suggest that curcumin could mitigate heat-induced effects on laying hens by altering the miRNA expression profiles of serum exosomes along with related regulatory pathways.
The Postharvest Safety and Quality of Fresh Basil as Affected by the Use of Cypriot Oregano (Origanum dubium) Extracts
Authors: Xylia Panayiota , Chrysargyris Antonios , Tzortzakis Nikolaos
Published: February 2024

The use of natural products (including essential oil—EO from medicinal and aromatic plants) on fresh commodities such as leafy greens has gained a lot of attention due to the beneficial effects of those products. However, fresh herbs are highly perishable commodities, and very little is known for their postharvest preservation. The present study aimed to (i) investigate the effects of Cypriot oregano (Origanum dubium) EO and hydrosol (at different concentrations and times of application) on fresh basil’s quality attributes and preservation and (ii) examine the efficacy of selected doses of O. dubium EO and hydrosol against two major foodborne pathogens inoculated on fresh basil stored at 4 °C for six days. The results of the current study indicated that the application of O. dubium EO at high concentrations in combination with a longer dipping time presented a less aromatic (less basil-like aroma) and preferable commodity, whereas hydrosol application resulted in a more acceptable and marketable commodity. In addition, an increase in antioxidant capacity and ascorbic acid content were observed with the EO, whilst hydrosol application was found to decrease basil’s antioxidant capacity. Both investigated products (EO and hydrosol) where found to present great antibacterial activity against Salmonella enterica and Listeria monocytogenes inoculated on fresh basil even six days after the application. Overall, the investigated natural products (i.e., O. dubium EO and hydrosol) could be considered alternative sanitizing agents during the postharvest processing of fresh basil, whilst preserving and/or improving its nutritional value (i.e., an increase in antioxidants or flavonoids). However, caution should be taken when using it at high concentrations; thus, further research is needed for future commercial-scale use and on other fresh produce.
Using Photovoice to Explore Determinants of Health among Homeless and Unstably Housed Women
Authors: Mackelprang Jessica L. , Graves Janessa M. , Schulz Halle M.
Published: February 2024

The lived experiences of homeless and unstably housed women, including their health-related priorities, are understudied in smaller metropolitan and rural communities. In this study, we partnered with a day center for women who experience homelessness in Spokane, Washington. We used Photovoice, a community-based participatory action research method, to explore the health-related concerns, needs, and behavior of women who are homeless or unstably housed. Participant-generated photographs and group interview data were analyzed using thematic analysis. Three themes were generated: “These are my supports”, “I’m trying to make my health better”, and “[My] choices are very limited”. The themes illustrated individual, interpersonal, community, and societal strengths and vulnerabilities aligned with the social ecological model. Participants demonstrated resourcefulness, creativity, and hope as they strived toward health improvement. Trauma-informed, strengths-based approaches that respect the autonomy of homeless and unstably housed women and that amplify their voices are needed to minimize power imbalances in research, policy, and practice. This includes an imperative for healthcare and social work programs to ready graduates to deliver effective, empathic services by increasing their knowledge of social determinants of health and of the stigma faced by marginalized communities. Moreover, collaborating with these communities when designing, implementing, and evaluating services is critical.
The Effect of the COVID-19 Pandemic on Palestinian Patients Attending Selected Governmental Hospitals: An Analysis of Hospital Records
Authors: Atary Mousa , Abu-Rmeileh Niveen M. E.
Published: February 2024

Introduction: Confronting the COVID-19 epidemic forced the closure or relocation of the majority of health facilities. It is likely that non-COVID-19 patients suffered collateral effects. Methods: The clinic and operating room records were analyzed at selected Palestinian government hospitals in the West Bank region. Results: The reduction in patient clinic visits varied from 49% to 90%, with Ear-Nose-Throat (ENT), urology, and pediatric clinics being the most affected. The reduction in operation numbers in the center (which had independent decision-making) ranged from 7.1% to 23.4%, but in the north and south (which followed centralized choices), the reduction ranged from 19.6% to 91.8%. Conclusions: COVID-19 affected outpatient visits. The pandemic affected some services, but West Bank hospitals were able to provide normal obstetric and gynecological treatments and help patients who needed primary or intermediate surgery.
Development and Psychometric Test of the Salutogenic Survey on Sustainable Working Life for Nurses: Identifying Resistance Resources against Stress
Authors: Eriksson Monica , Johannesson Elias , Kerekes Nóra , Emilsson Maria , Pennbrant Sandra , Nunstedt Håkan
Published: February 2024

Extensive research shows nurses’ work environment to be particularly stressful. This study develops, explores, and psychometrically tests a new profession-specific questionnaire identifying generalised and specific resistance resources, that make it possible to measure resources to manage work-related stress. An exploratory study design was employed. The questionnaire development was inspired by the MEASURE approach and the salutogenic theory of health. Building on the results from a literature review of nursing research and salutogenesis, supplemented by twelve interviews with hospital nurses, an item pool was generated. The first version was pilot-tested in a group of nurses who were studying to become specialist nurses. The second version of the questionnaire was psychometrically tested on a sample of registered nurses in close patient care (n = 475), analysed using confirmatory factor analysis to test seven predefined domains of the questionnaire. The analysis revealed a first order seven-domain model of 21 items: job satisfaction, professional role, work motivation, commitment, belonging in the workplace, factors and conditions for remaining in the profession, and workload. The structure of the questionnaire indicates its usefulness in clinical practice for measuring resistance resources.
Silymarin Reduced Insulin Resistance in Non-Diabetic Women with Obesity
Authors: MacDonald-Ramos Karla , Monroy Adriana , Bobadilla-Bravo Mariana , Cerbón Marco
Published: February 2024

Silymarin has ameliorated obesity, type 2 diabetes (T2DM), and insulin resistance (IR) in combination with standard therapy, diet, or exercise in recent studies. Obesity and IR are the main risk factors for developing T2DM and other metabolic disorders. Today, there is a need for new strategies to target IR in patients with these metabolic diseases. In the present longitudinal study, a group of non-diabetic insulin-resistant women with type 1 and type 2 obesity were given silymarin for 12 weeks, with no change in habitual diet and physical activity. We used the Homeostatic Model Assessment for Insulin Resistance Index (HOMA-IR) to determine IR at baseline and after silymarin treatment (t = 12 weeks). We obtained five timepoint oral glucose tolerance tests, and other biochemical and clinical parameters were analyzed before and after treatment. Treatment with silymarin alone significantly reduced mean fasting plasma glucose (FPG) and HOMA-IR levels at 12 weeks compared to baseline values (p < 0.05). Mean fasting plasma insulin (FPI), total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (Tg), indirect bilirubin, and C-reactive protein (CRP) levels decreased compared to baseline values, although changes were non-significant. The overall results suggest that silymarin may offer a therapeutic alternative to improve IR in non-diabetic individuals with obesity. Further clinical trials are needed in this type of patient to strengthen the results of this study.
Thrombelastography and Conventional Coagulation Markers in Chronic Obstructive Pulmonary Disease: A Prospective Paired-Measurements Study Comparing Exacerbation and Stable Phases
Authors: Rastoder Ema , Kamstrup Peter , Hedsund Caroline , Jordan Alexander , Sivapalan Pradeesh , Rømer Valdemar , Falkvist Frederikke , Hamidi Sadaf , Bendstrup Elisabeth , Sperling Søren , Dons Maria , Biering-Sørensen Tor , Falster Casper , Laursen Christian B. , Carlsen Jørn , Jensen Jens-Ulrik Stæhr
Published: February 2024

Chronic Obstructive Pulmonary Disease (COPD) exacerbation is known for its substantial impact on morbidity and mortality among affected patients, creating a significant healthcare burden worldwide. Coagulation abnormalities have emerged as potential contributors to exacerbation pathogenesis, raising concerns about increased thrombotic events during exacerbation. The aim of this study was to explore the differences in thrombelastography (TEG) parameters and coagulation markers in COPD patients during admission with exacerbation and at a follow-up after discharge. This was a multi-center cohort study. COPD patients were enrolled within 72 h of hospitalization. The baseline assessments were Kaolin-TEG and blood samples. Statistical analysis involved using descriptive statistics; the main analysis was a paired t-test comparing coagulation parameters between exacerbation and follow-up. One hundred patients participated, 66% of whom were female, with a median age of 78.5 years and comorbidities including atrial fibrillation (18%) and essential arterial hypertension (45%), and sixty-five individuals completed a follow-up after discharge. No significant variations were observed in Kaolin-TEG or conventional coagulation markers between exacerbation and follow-up. The Activated Partial Thromboplastin Clotting Time (APTT) results were near-significant, with p = 0.08. In conclusion, TEG parameters displayed no significant alterations between exacerbation and follow-up.
Mitochondrial Quantity and Quality in Age-Related Sarcopenia
Authors: Marzetti Emanuele , Calvani Riccardo , Coelho-Júnior Hélio José , Landi Francesco , Picca Anna
Published: February 2024

Sarcopenia, the age-associated decline in skeletal muscle mass and strength, is a condition with a complex pathophysiology. Among the factors underlying the development of sarcopenia are the progressive demise of motor neurons, the transition from fast to slow myosin isoform (type II to type I fiber switch), and the decrease in satellite cell number and function. Mitochondrial dysfunction has been indicated as a key contributor to skeletal myocyte decline and loss of physical performance with aging. Several systems have been implicated in the regulation of muscle plasticity and trophism such as the fine-tuned and complex regulation between the stimulator of protein synthesis, mechanistic target of rapamycin (mTOR), and the inhibitor of mTOR, AMP-activated protein kinase (AMPK), that promotes muscle catabolism. Here, we provide an overview of the molecular mechanisms linking mitochondrial signaling and quality with muscle homeostasis and performance and discuss the main pathways elicited by their imbalance during age-related muscle wasting. We also discuss lifestyle interventions (i.e., physical exercise and nutrition) that may be exploited to preserve mitochondrial function in the aged muscle. Finally, we illustrate the emerging possibility of rescuing muscle tissue homeostasis through mitochondrial transplantation.
Special Issue: “Novel Researches and Perspectives on Prostate Cancer”
Authors: Beretta Giovanni Luca
Published: February 2024

Prostate cancer (PCa) represents the second most diagnosed tumor and the fifth most common cause of cancer death in men globally [...]
Therapeutic Targeting of Hypoxia-Inducible Factors in Cancer
Authors: Musleh Ud Din Saba , Streit Spencer G. , Huynh Bao Tran , Hana Caroline , Abraham Anna-Ninny , Hussein Atif
Published: February 2024

In the realm of cancer therapeutics, targeting the hypoxia-inducible factor (HIF) pathway has emerged as a promising strategy. This study delves into the intricate web of HIF-associated mechanisms, exploring avenues for future anticancer therapies. Framing the investigation within the broader context of cancer progression and hypoxia response, this article aims to decipher the pivotal role played by HIF in regulating genes influencing angiogenesis, cell proliferation, and glucose metabolism. Employing diverse approaches such as HIF inhibitors, anti-angiogenic therapies, and hypoxia-activated prodrugs, the research methodologically intervenes at different nodes of the HIF pathway. Findings showcase the efficacy of agents like EZN-2968, Minnelide, and Acriflavine in modulating HIF-1α protein synthesis and destabilizing HIF-1, providing preliminary proof of HIF-1α mRNA modulation and antitumor activity. However, challenges, including toxicity, necessitate continued exploration and development, as exemplified by ongoing clinical trials. This article concludes by emphasizing the potential of targeted HIF therapies in disrupting cancer-related signaling pathways.
The Pivotal Role of Oxytocin’s Mechanism of Thermoregulation in Prader-Willi Syndrome, Schaaf-Yang Syndrome, and Autism Spectrum Disorder
Authors: Camerino Claudia
Published: February 2024

Oxytocin (Oxt) regulates thermogenesis, and altered thermoregulation results in Prader-Willi syndrome (PWS), Schaaf-Yang syndrome (SYS), and Autism spectrum disorder (ASD). PWS is a genetic disorder caused by the deletion of the paternal allele of 15q11-q13, the maternal uniparental disomy of chromosome 15, or defects in the imprinting center of chromosome 15. PWS is characterized by hyperphagia, obesity, low skeletal muscle tone, and autism spectrum disorder (ASD). Oxt also increases muscle tonicity and decreases proteolysis while PWS infants are hypotonic and require assisted feeding in early infancy. This evidence inspired us to merge the results of almost 20 years of studies and formulate a new hypothesis according to which the disruption of Oxt’s mechanism of thermoregulation manifests in PWS, SYS, and ASD through thermosensory abnormalities and skeletal muscle tone. This review will integrate the current literature with new updates on PWS, SYS, and ASD and the recent discoveries on Oxt’s regulation of thermogenesis to advance the knowledge on these diseases.
The Multienzyme Complex Nature of Dehydroepiandrosterone Sulfate Biosynthesis
Authors: Tumilovich Anastasiya , Yablokov Evgeniy , Mezentsev Yuri , Ershov Pavel , Basina Viktoriia , Gnedenko Oksana , Kaluzhskiy Leonid , Tsybruk Tatsiana , Grabovec Irina , Kisel Maryia , Shabunya Polina , Soloveva Natalia , Vavilov Nikita , Gilep Andrei , Ivanov Alexis
Published: February 2024

Dehydroepiandrosterone (DHEA), a precursor of steroid sex hormones, is synthesized by steroid 17-alpha-hydroxylase/17,20-lyase (CYP17A1) with the participation of microsomal cytochrome b5 (CYB5A) and cytochrome P450 reductase (CPR), followed by sulfation by two cytosolic sulfotransferases, SULT1E1 and SULT2A1, for storage and transport to tissues in which its synthesis is not available. The involvement of CYP17A1 and SULTs in these successive reactions led us to consider the possible interaction of SULTs with DHEA-producing CYP17A1 and its redox partners. Text mining analysis, protein–protein network analysis, and gene co-expression analysis were performed to determine the relationships between SULTs and microsomal CYP isoforms. For the first time, using surface plasmon resonance, we detected interactions between CYP17A1 and SULT2A1 or SULT1E1. SULTs also interacted with CYB5A and CPR. The interaction parameters of SULT2A1/CYP17A1 and SULT2A1/CYB5A complexes seemed to be modulated by 3′-phosphoadenosine-5′-phosphosulfate (PAPS). Affinity purification, combined with mass spectrometry (AP-MS), allowed us to identify a spectrum of SULT1E1 potential protein partners, including CYB5A. We showed that the enzymatic activity of SULTs increased in the presence of only CYP17A1 or CYP17A1 and CYB5A mixture. The structures of CYP17A1/SULT1E1 and CYB5A/SULT1E1 complexes were predicted. Our data provide novel fundamental information about the organization of microsomal CYP-dependent macromolecular complexes.
Cloning and Characterization of Chitin Deacetylase from Euphausia superba
Authors: Wang Xutong , Tan Jiahao , Zou Huaying , Wang Fang , Xu Jiakun
Published: February 2024

Chitin deacetylase (CDA) can catalyze the deacetylation of chitin to produce chitosan. In this study, we identified and characterized a chitin deacetylase gene from Euphausia superba (EsCDA-9k), and a soluble recombinant protein chitin deacetylase from Euphausia superba of molecular weight 45 kDa was cloned, expressed, and purified. The full-length cDNA sequence of EsCDA-9k was 1068 bp long and encoded 355 amino acid residues that contained the typical domain structure of carbohydrate esterase family 4. The predicted three-dimensional structure of EsCDA-9k showed a 67.32% homology with Penaeus monodon. Recombinant chitin deacetylase had the highest activity at 40 °C and pH 8.0 in Tris-HCl buffer. The enzyme activity was enhanced by metal ions Co2+, Fe3+, Ca2+, and Na+, while it was inhibited by Zn2+, Ba2+, Mg2+, and EDTA. Molecular simulation of EsCDA-9k was conducted based on sequence alignment and homology modeling. The EsCDA-9k F18G mutant showed a 1.6-fold higher activity than the wild-type enzyme. In summary, this is the first report of the cloning and heterologous expression of the chitin deacetylase gene in Euphausia superba. The characterization and function study of EsCDA-9k will serve as an important reference point for future application.
Pyrimidine Schiff Bases: Synthesis, Structural Characterization and Recent Studies on Biological Activities
Authors: Bryndal Iwona , Stolarczyk Marcin , Mikołajczyk Aleksandra , Krupińska Magdalena , Pyra Anna , Mączyński Marcin , Matera-Witkiewicz Agnieszka
Published: February 2024

Recently, 5-[(4-ethoxyphenyl)imino]methyl-N-(4-fluorophenyl)-6-methyl-2-phenylpyrimidin-4-amine has been synthesized, characterized, and evaluated for its antibacterial activity against Enterococcus faecalis in combination with antineoplastic activity against gastric adenocarcinoma. In this study, new 5-iminomethylpyrimidine compounds were synthesized which differ in the substituent(s) of the aromatic ring attached to the imine group. The structures of newly obtained pyrimidine Schiff bases were established by spectroscopy techniques (ESI-MS, FTIR and 1H NMR). To extend the current knowledge about the features responsible for the biological activity of the new 5-iminomethylpyrimidine derivatives, low-temperature single-crystal X-ray analyses were carried out. For all studied crystals, intramolecular N–H∙∙∙N hydrogen bonds and intermolecular C–H∙∙∙F interactions were observed and seemed to play an essential role in the formation of the structures. Simultaneously, their biological properties based on their cytotoxic features were compared with the activities of the Schiff base (III) published previously. Moreover, computational investigations, such as ADME prediction analysis and molecular docking, were also performed on the most active new Schiff base (compound 4b). These results were compared with the highest active compound III.
Comprehensive Characterization of the C3HC4 RING Finger Gene Family in Potato (Solanum tuberosum L.): Insights into Their Involvement in Anthocyanin Biosynthesis
Authors: Chen Limin , Li Yuanming , Zhu Jinyong , Li Zhitao , Wang Weilu , Qi Zheying , Li Dechen , Yao Panfeng , Bi Zhenzhen , Sun Chao , Liu Yuhui , Liu Zhen
Published: February 2024

The C3HC4 RING finger gene (RING-HC) family is a zinc finger protein crucial to plant growth. However, there have been no studies on the RING-HC gene family in potato. In this study, 77 putative StRING-HCs were identified in the potato genome and grouped into three clusters based on phylogenetic relationships, the chromosome distribution, gene structure, conserved motif, gene duplication events, and synteny relationships, and cis-acting elements were systematically analyzed. By analyzing RNA-seq data of potato cultivars, the candidate StRING-HC genes that might participate in tissue development, abiotic stress, especially drought stress, and anthocyanin biosynthesis were further determined. Finally, a StRING-HC gene (Soltu.DM.09G017280 annotated as StRNF4-like), which was highly expressed in pigmented potato tubers was focused on. StRNF4-like localized in the nucleus, and Y2H assays showed that it could interact with the anthocyanin-regulating transcription factors (TFs) StbHLH1 of potato tubers, which is localized in the nucleus and membrane. Transient assays showed that StRNF4-like repressed anthocyanin accumulation in the leaves of Nicotiana tabacum and Nicotiana benthamiana by directly suppressing the activity of the dihydroflavonol reductase (DFR) promoter activated by StAN1 and StbHLH1. The results suggest that StRNF4-like might repress anthocyanin accumulation in potato tubers by interacting with StbHLH1. Our comprehensive analysis of the potato StRING-HCs family contributes valuable knowledge to the understanding of their functions in potato development, abiotic stress, hormone signaling, and anthocyanin biosynthesis.
The Transcription Factor SsZNC1 Mediates Virulence, Sclerotial Development, and Osmotic Stress Response in Sclerotinia sclerotiorum
Authors: Huang Yongkun , Zhaxi Zhima , Fu Yanping , Xie Jiatao , Chen Tao , Li Bo , Yu Xiao , Lin Yang , Jiang Daohong , Cheng Jiasen
Published: February 2024

Sclerotinia sclerotiorum is a fungal pathogen with a broad range of hosts, which can cause diseases and pose a great threat to many crops. Fungal-specific Zn2Cys6 transcription factors (TFs) constitute a large family prevalent among plant pathogens. However, the function of Zn2Cys6 TFs remains largely unknown. In this study, we identified and characterized SsZNC1, a Zn2Cys6 TF in S. sclerotiorum, which is involved in virulence, sclerotial development, and osmotic stress response. The expression of SsZNC1 was significantly up-regulated in the early stages of S. sclerotiorum infection on Arabidopsis leaves. The target deletion of SsZNC1 resulted in reduced virulence on Arabidopsis and oilseed rape. In addition, sclerotial development ability and growth ability under hyperosmotic conditions of SsZNC1 knockout transformants were reduced. A transcriptomic analysis unveiled its regulatory role in key cellular functions, including cellulose catabolic process, methyltransferase activity, and virulence, etc. Together, our results indicated that SsZNC1, a core regulatory gene involved in virulence, sclerotial development and stress response, provides new insight into the transcription regulation and pathogenesis of S. sclerotiorum.
A Quantitative Analysis on the Coordination of Regional Ecological and Economic Development Based on the Ecosystem Service Evaluation
Authors: Li Shuxiang , Ma Shuhua
Published: February 2024

The coordination between regional ecological and economic development has become a crucial topic in current environmental and development research due to the establishment of sustainable development goals and the acceleration of urbanization. In this study, an improved eco-economy coordination (EEC) model is proposed to evaluate the coordination of regional ecological and economic development. This study focuses on Wuhan, China, and utilizes socioeconomic and remote sensing data from 2000 to 2015 to measure both static and dynamic ecosystem service values (ESV). ESVs are the direct and indirect benefits produced by ecosystems that support human survival and development. The calculated ESVs are then integrated into the ecological–economic coordination (EEC) evaluation. This study aims to conduct a comparative analysis of the ecological-economic coordination across various districts of Wuhan, with a focus on spatiotemporal factors. In addition, this paper highlights the role of environmental adjustment coefficients in enhancing the EEC assessment. The results show that (1) the total static ESV experienced a loss of RMB 1.2 billion (approximately USD 169 million) and showed a decreasing trend, while the dynamically corrected ESV reversed this trend; and (2) EEC based on static ESV showed a low degree of conflict between ecological and economic reconciliation compared with a significant increase in EEC based on dynamic ESV. Based on static ESV, the EEC showed a low degree of conflict between ecological and economic reconciliation compared to a significant increase in EEC based on dynamic ESV. After being corrected by environmental adjustment coefficients, the EEC values showed a more differentiated distribution. Most regions demonstrated an overall upward trend in EEC, with a few, such as Wuchang District and Jiang’an District, being notable examples. However, Jianghan District presented a high conflict status. Finally, a series of decision-making suggestions are given based on relevant study results, and an important policy implication is that the coordination of economic growth and ecological protection, under large downward pressure from EEC values, needs to be paid special attention in policy decision-making.
Understanding the Impact of Sheep Digestion on Seed Germination in the Cold Desert Annual Diptychocarpus strictus with Emphasis on Fruit and Seed Heteromorphism
Authors: Zhao Xuheng , Wang Yixiang , Cao Fangfang , Wang Xuzhe , Zhang Fanfan , Anwar Sumera , Sun Zhihua , He Hongsu
Published: February 2024

This study aimed to investigate the morphological characteristics of fruits and seeds from Diptychocarpus strictus, a plant species inhabiting the cold desert pastoral area of China. Furthermore, this study sought to evaluate the germination potential of these seeds following digestion by sheep. This study employed the sheep rumen fistula method to simulate rumen digestion at various time intervals. Subsequently, an in vitro simulation method was utilized to simulate true gastric and intestinal digestion after rumen digestion. Paper germination tests were then conducted to assess the impact of the digestive process on the heteromorphic seed morphology and germination. During rumen digestion, the seeds were protected by wide wings. The results revealed a highly significant negative correlation (p < 0.01) between seed wing length and digestion time. Post-rumen digestion, variations in the germination rate among seeds from fruits at different locations were observed. Indicators, such as germination rate, exhibited a highly significant negative correlation with rumen digestion time (p < 0.01). In vitro simulated digestion tests demonstrated that Diptychocarpus strictus seeds retained their ability to germinate even after complete digestion within the livestock’s digestive tract. The polymorphic nature of Diptychocarpus strictus seeds, coupled with their capacity to survive and germinate through the digestive tract, facilitates the spread of these seeds. This finding has implications for mitigating desert grassland degradation and promoting sustainable ecological development.
Key Factors Shaping Green Logistics in Thailand’s Auto Industry: An Application of Structural Equation Modeling
Authors: Youngswaing Wuttichai , Jomnonkwao Sajjakaj , Cheunkamon Ekkapong , Ratanavaraha Vatanavongs
Published: February 2024

Background: Environmental responsibility is a critical focus in business and production. International partners have urged Thai businesses to adhere to global standards in environmental and social accountability. Green logistics management focuses on minimizing costs and boosting competitive efficiency in the industrial sector, while also promoting environmental protection. This study investigates the factors influencing green logistics in the motor vehicle industry. Methods: A self-administered questionnaire was used for data collection from 1638 participants. Confirmatory factor analysis and structural equation modeling were employed to identify causal links impacting green logistics effectiveness. Results: Findings indicate that government and organizational environmental policies, a company’s financial ability to implement green initiatives, commitment to eco-friendly investment, and public environmental attitudes significantly and directly affect the efficiency of green logistics. Conclusions: This research provides valuable insights for car manufacturers into key factors in green logistics management. These insights can help in developing strategic plans to achieve environmental sustainability in the context of future market competition.
Cs+ Promoting the Diffusion of K+ and Inhibiting the Generation of Newberyite in Struvite-K Cements: Experiments and Molecular Dynamics Simulation Calculations
Authors: Leng Difei , Fu Qiuyan , Ge Yunlu , He Chenhao , Lv Yang , Li Xiangguo
Published: February 2024

Struvite-K cements, also called magnesium potassium phosphate cements (MKPCs), are applicable for particular applications, especially the immobilization of radioactive Cs+ in the nuclear industry. This work focuses on how Cs+ affects the hydration mechanism of struvite-K cements because newberyite and brucite in the hydration products are deemed to be risky products that result in cracking. Experiments and molecular dynamics simulations showed that Cs+ promoted the diffusion of K+ to the surface of MgO, which greatly facilitates the formation of more K-struvite crystals, inhibiting the formation of newberyite and brucite. A total of 0.02 M Cs+ resulted in a 40.44%, 13.93%, 60.81%, and 32.18% reduction in the amount of newberyite and brucite, and the Cs immobilization rates were 99.07%, 99.84%, 99.87%, and 99.83% when the ratios of Mg/P were 1, 3, 5, and 7, respectively. This provides new evidence of stability for struvite-K cements on radioactive Cs+ immobilization. Surprisingly, another new crystal, [CsPO3·H2O]4, was found to be a dominating Cs-containing phase in Cs-immobilizing struvite-K cements, in addition to Cs-struvite.
Assessing the Impact of the COVID-19 Pandemic on Pediatric Emergency Department Visits in Taiwan
Authors: Lee Yu-Ting , Lai Yen-Wen , Chen Jiann-Hwa , Chen Wei-Lung , Wu Meng-Yu , Chung Jui-Yuan
Published: February 2024

Background and Objectives: The coronavirus disease 2019 (COVID-19) pandemic has profoundly impacted healthcare systems worldwide. To assess the effects of the pandemic on pediatric emergency department (ED) visits in Taiwan, we conducted a study to evaluate changes in pediatric ED visits during the COVID-19 pandemic. Materials and Methods: This retrospective study included pediatric patients (age ≤ 18) who visited the ED between 21 January 2019 and 30 April 2019, at three hospitals of the Cathay Health System, and compared them with a corresponding period in 2020. Basic information, including mode of arrival, triage level, disposition, chief complaints, and incidence rates, were analyzed before and during the pandemic. Results: A total of 10,116 patients, with 6009 in the pre-pandemic group and 4107 in the pandemic group, were included in this study. The mean number of daily pediatric ED visits decreased from 60.09 before the pandemic to 40.66 during the pandemic, while ambulance use increased significantly by 2.56%. The percentage of patients with high acuity triage levels (levels 1 and 2) was significantly lower during the pandemic period (0.63% and 10.18%, respectively) than the pre-pandemic period (0.7% and 10.9%, respectively). Additionally, a significantly higher proportion of patients were discharged during the pandemic period (89.36%) than during the pre-pandemic period (88.33%). The proportion of COVID-19-related complaints, such as fever and respiratory tract infections, as well as other complaints including gastrointestinal issues, trauma, and psychological problems, significantly increased during the pandemic. Conclusions: In preparation for future pandemics, we recommend increasing emergency medical service capacity, establishing a non-contagious route for obtaining chronic medication prescriptions, optimizing staff allocation in pediatric emergency departments, and increasing the number of hospital social workers for enhanced support.
16S rRNA Next-Generation Sequencing May Not Be Useful for Examining Suspected Cases of Spontaneous Bacterial Peritonitis
Authors: Yang Chan Jin , Song Ju Sun , Yoo Jeong-Ju , Park Keun Woo , Yun Jina , Kim Sang Gyune , Kim Young Seok
Published: February 2024

Background and Objectives: Ascites, often associated with liver cirrhosis, poses diagnostic challenges, particularly in detecting bacterial infections. Traditional methods have limitations, prompting the exploration of advanced techniques such as 16S rDNA next-generation sequencing (NGS) for improved diagnostics in such low-biomass fluids. The aim of this study was to investigate whether the NGS method enhances detection sensitivity compared to a conventional ascites culture. Additionally, we aimed to explore the presence of a microbiome in the abdominal cavity and determine whether it has a sterile condition. Materials and Methods: Ten patients with clinically suspected spontaneous bacterial peritonitis (SBP) were included in this study. A traditional ascites culture was performed, and all ascites samples were subjected to 16S ribosomal RNA gene amplification and sequencing. 16S rRNA gene sequencing results were interpreted by comparing them to positive and negative controls for each sample. Results: Differential centrifugation was applied to all ascites samples, resulting in very small or no bacterial pellets being harvested. The examination of the 16S amplicon sequencing libraries indicated that the target amplicon products were either minimally visible or exhibited lower intensity than their corresponding negative controls. Contaminants present in the reagents were also identified in the ascites samples. Sequence analysis of the 16S rRNA gene of all samples showed microbial compositions that were akin to those found in the negative controls, without any bacteria isolated that were unique to the samples. Conclusions: The peritoneal cavity and ascites exhibit low bacterial biomass even in the presence of SBP, resulting in a very low positivity rate in 16S rRNA gene sequencing. Hence, the 16S RNA sequencing method does little to enhance the rate of positive samples compared to traditional culture methods, including in SBP cases.
The Diversity of the Linguofacial Trunk
Authors: Dumitru Cătălin Constantin , Vrapciu Alexandra Diana , Rusu Mugurel Constantin
Published: February 2024

Background and Objectives: Typically, the external carotid artery (ECA) sends off separate anterior branches: the superior thyroid, lingual, and facial arteries. These could, however, form common trunks: thyrolinguofacial, linguofacial (LFT), or thyrolingual. Although known, the LFT variant was poorly detailed previously, and most authors just counted the variant. We aimed to demonstrate the individual anatomical possibilities of the LFT on a case-by-case basis. Materials and Methods: 150 archived angioCT files were used. After applying inclusion and exclusion criteria, 147 files of 86 males and 61 females were kept for this study. Results: In 34/147 cases, LFTs were found (23.12%). Bilateral LFTs were found in 13/34 cases (38.24%) and unilateral LFTs in 21/34 (61.76%) cases. Forty-seven LFTs were thus identified and further studied for different variables. Regarding the vertical topography of LFT origin, type 1a (suprahyoid and infragonial) was found in 28 LFTs (59.57%), type 1b (suprahyoid and gonial) was found in eight LFTs (17.02%), type 3 (suprahyoid and supragonial) was found in two LFTs (4.25%), type 2 (hyoid level of origin) in eight LFTs (17.02%), and type 3 (infrahyoid origin) in just one LFT (2.12%). Types of the initial course of the LFT were determined: type I, ascending, was found in 22/47 LFTs; type II, descending, in 12/47 LFTs; and type III, transverse, in 13/47 LFTs. Regarding the orientation of the first loop of the LFT, 23/47 LFTs had no loop, 4/47 had anterior loops, 1/47 had a posterior loop, 5/47 had superior loops, 5/47 had inferior loops, and 9/47 had medial loops. The position of the LFT relative to the ECA was classified as medial, anterior, or antero-medial. An amount of 12/47 LFTs were anterior to the ECA, 22/47 were antero-medial, 10/47 were medial, 2/47 were inferior, and 1/47 was lateral. Regarding their general morphology, 23/47 LFTs had a rectilinear course, 22/47 had loops, and 2/47 were coiled. A case-by-case presentation of results further demonstrated the diversity of the LFT. Conclusions: In conclusion, the morphology and topography of the LFT are individually specific and unpredictable. It can be anticipated case-by-case by surgeons on CT or MR angiograms.
A Compact Memristor Model Based on Physics-Informed Neural Networks
Authors: Lee Younghyun , Kim Kyeongmin , Lee Jonghwan
Published: February 2024

Memristor devices have diverse physical models depending on their structure. In addition, the physical properties of memristors are described using complex differential equations. Therefore, it is necessary to integrate the various models of memristor into an unified physics-based model. In this paper, we propose a physics-informed neural network (PINN)-based compact memristor model. PINNs can solve complex differential equations intuitively and with ease. This methodology is used to conduct memristor physical analysis. The weight and bias extracted from the PINN are implemented in a Verilog-A circuit simulator to predict memristor device characteristics. The accuracy of the proposed model is verified using two memristor devices. The results show that PINNs can be used to extensively integrate memristor device models.
Novel SiC Trench MOSFET with Improved Third-Quadrant Performance and Switching Speed
Authors: Ou Yangjie , Lan Zhong , Hu Xiarong , Liu Dong
Published: February 2024

A SiC double-trench MOSFET embedded with a lower-barrier diode and an L-shaped gate-source in the gate trench, showing improved reverse conduction and an improved switching performance, was proposed and studied with 2-D simulations. Compared with a double-trench MOSFET (DT-MOS) and a DT-MOS with a channel-MOS diode (DTC-MOS), the proposed MOS showed a lower voltage drop (VF) at IS = 100 A/cm2, which can prevent bipolar degradation at the same blocking voltage (BV) and decrease the maximum oxide electric field (Emox). Additionally, the gate–drain capacitance (Cgd) and gate–drain charge (Qgd) of the proposed MOSFET decreased significantly because the source extended to the bottom of the gate, and the overlap between the gate electrode and drain electrode decreased. Although the proposed MOS had a greater Ron,sp than the DT-MOS and DTC-MOS, it had a lower switching loss and greater advantages for high-frequency applications.
An Update of Kaempferol Protection against Brain Damage Induced by Ischemia-Reperfusion and by 3-Nitropropionic Acid
Authors: López-Sánchez Carmen , Lagoa Ricardo , Poejo Joana , García-López Virginio , García-Martínez Virginio , Gutierrez-Merino Carlos
Published: February 2024

Kaempferol, a flavonoid present in many food products, has chemical and cellular antioxidant properties that are beneficial for protection against the oxidative stress caused by reactive oxygen and nitrogen species. Kaempferol administration to model experimental animals can provide extensive protection against brain damage of the striatum and proximal cortical areas induced by transient brain cerebral ischemic stroke and by 3-nitropropionic acid. This article is an updated review of the molecular and cellular mechanisms of protection by kaempferol administration against brain damage induced by these insults, integrated with an overview of the contributions of the work performed in our laboratories during the past years. Kaempferol administration at doses that prevent neurological dysfunctions inhibit the critical molecular events that underlie the initial and delayed brain damage induced by ischemic stroke and by 3-nitropropionic acid. It is highlighted that the protection afforded by kaempferol against the initial mitochondrial dysfunction can largely account for its protection against the reported delayed spreading of brain damage, which can develop from many hours to several days. This allows us to conclude that kaempferol administration can be beneficial not only in preventive treatments, but also in post-insult therapeutic treatments.
Molecular-Simulation–Inspired Synthesis of [6]-Prismane via Photoisomerisation of Octafluoro[2.2]paracyclophane
Authors: Hosokawa Yoichi , Kajiya Shuji , Ohshima Ayako , Kawata Satoshi , Ishida Nobuhiro , Usuki Arimitsu
Published: February 2024

Prismanes have been attracting interest for nearly 50 years because of their geometric symmetry, highly strained structures, and unique applications due to their high carbon densities and bulky structures. Although [3]-, [4]-, and [5]-prismanes have been synthesised, [6]-prismanes and their derivatives remain elusive. Herein, fluorine chemistry, molecular mechanics, molecular orbital package, and density functional theory calculations were used to design and implement the photoisomerisation of octafluoro[2.2]paracyclophane (selected based on the good overlap of its lowest unoccupied molecular orbitals and short distance between the benzene rings) into octafluoro-[6]-prismane. Specifically, a dilute solution of the above precursor in CH3CN/H2O/dimethyl sulfoxide (DMSO) (2:1:8, v/v/v) solution was irradiated with ultraviolet light, with the formation of the desired product confirmed through the use of nuclear magnetic resonance spectroscopy and gas chromatography–mass spectrometry. The product was thermally stable in solution but not under work-up conditions, which complicated the further analysis and single-crystal preparation. The key criteria for successful photoisomerisation were the presence of fluorine substituents in the cyclophane structure and DMSO in the solvent system. A more stable derivative design requires the isolation of prismane products. The proposed fluorination-based synthetic strategy is applicable to developing novel high-strain molecules/materials with three-dimensional skeletons.