bluuebunny/crossref_metadata_2025 huggingface dataset:
A survey on detecting healthcare concept drift in AI/ML models from a finance perspective
Authors: M. S. Abdul Razak , C. R. Nirmala , B. R. Sreenivasa , Lahza Husam , Lahza Hassan Fareed M.
Published: April 2023

Data is incredibly significant in today's digital age because data represents facts and numbers from our regular life transactions. Data is no longer arriving in a static form; it is now arriving in a streaming fashion. Data streams are the arrival of limitless, continuous, and rapid data. The healthcare industry is a major generator of data streams. Processing data streams is extremely complex due to factors such as volume, pace, and variety. Data stream classification is difficult owing to idea drift. Concept drift occurs in supervised learning when the statistical properties of the target variable that the model predicts change unexpectedly. We focused on solving various forms of concept drift problems in healthcare data streams in this research, and we outlined the existing statistical and machine learning methodologies for dealing with concept drift. It also emphasizes the use of deep learning algorithms for concept drift detection and describes the various healthcare datasets utilized for concept drift detection in data stream categorization.
Greening the ocean economy
Authors: Barbier Edward B.
Published: April 2023

The ocean economy comprises a wide range of industries—fishing, offshore energy, mineral extraction, shipping and coastal tourism. It generates $1.5 trillion in global value added and is expected to double in size by 2030. If we are to successfully green the ocean economy, we must overcome two major distortions—the underpricing of marine capital and the underfunding of ocean and coastal conservation. Many important values provided by the marine environment are ignored or discounted in our decisions to exploit, convert and pollute our coastlines and seas. The funding gap between current financing of conservation, restoration and sustainable management of marine capital and the most critical funding needs is estimated at $120–$154 billion annually. We urgently need a new global agreement for oceans and coasts that has three principal aims: (1) Phasing out subsidies for fishing, extractive activities, and other ocean industries. (2) Implementing market-based incentives, management reforms, and other regulations to reduce any remaining ecological marine damages. (3) Using any financial savings and revenues generated to support global funds and investments for conserving, restoring and protecting marine capital in an inclusive manner. There should also be more participation by the private sector in developing global marine and in bridging the funding gap for marine conservation. It is estimated that major companies in ocean industries could raise an additional $83–$186 billion each year for marine conservation investment that would also benefit their financial interests and markets.
Type I interferons augment regulatory T cell polarization in concert with ancillary cytokine signals
Authors: Eskandari Siawosh K. , Allos Hazim , Safadi Jenelle M. , Sulkaj Ina , Sanders Jan S. F. , Cravedi Paolo , Ghobrial Irene M. , Berger Stefan P. , Azzi Jamil R.
Published: April 2023

In the transplant community, research efforts exploring endogenous alternatives to inducing tolerogenic allo-specific immune responses are much needed. In this regard, CD4 + FoxP3+ regulatory T cells (Tregs) are appealing candidates due to their intrinsic natural immunosuppressive qualities. To date, various homeostatic factors that dictate Treg survival and fitness have been elucidated, particularly the non-redundant roles of antigenic CD3ζ/T-cell-receptor, co-stimulatory CD28, and cytokine interleukin (IL-)2 dependent signaling. Many of the additional biological signals that affect Tregs remain to be elucidated, however, especially in the transplant context. Previously, we demonstrated an unexpected link between type I interferons (IFNs) and Tregs in models of multiple myeloma (MM)—where MM plasmacytes escaped immunological surveillance by enhancing type I IFN signaling and precipitating upregulated Treg responses that could be overturned with specific knockdown of type I IFN signaling. Here, we elaborated on these findings by assessing the role of type I IFN signaling (IFN-α and -β) on Treg homeostasis within an alloimmune context. Specifically, we studied the induction of Tregs from naïve CD4 T cells. Using in vitro and in vivo models of murine skin allotransplantation, we found that type I IFN indeed spatiotemporally enhanced the polarization of naïve CD4 T cells into FoxP3+ Tregs. Notably, however, this effect was not independent of, and rather co-dependent on, ancillary cytokine signals including IL-2. These findings provide evidence for the relevance of type I IFN pathway in modulating FoxP3+ Treg responses and, by extension, stipulate an additional means of facilitating Treg fitness via type I IFNs.
How do differences in Achilles’ tendon moment arm lengths affect muscle-tendon dynamics and energy cost during running?
Authors: Bennett Eric C. , Machado Esthevan , Fletcher Jared R.
Published: April 2023

Introduction

The relationship between the Achilles tendon moment arm length (ATMA) and the energy cost of running (Erun) has been disputed. Some studies suggest a short ATMA reduces Erun while others claim a long ATMA reduces Erun. For a given ankle joint moment, a short ATMA permits a higher tendon strain energy storage, whereas a long ATMA reduces muscle fascicle force and muscle energy cost but shortening velocity is increased, elevating the metabolic cost. These are all conflicting mechanisms to reduce Erun, since AT energy storage comes at a metabolic cost. Neither of these proposed mechanisms have been examined together.

Methods

We measured ATMA using the tendon travel method in 17 males and 3 females (24 ± 3 years, 75 ± 11 kg, 177 ± 7 cm). They ran on a motorized treadmill for 10 min at 2.5 m · s−1 while Erun was measured. AT strain energy storage, muscle lengths, velocities and muscle energy cost were calculated during time-normalized stance from force and ultrasound data. A short (SHORT n = 11, ATMA = 29.5 ± 2.0 mm) and long (LONG, n = 9, ATMA = 36.6 ± 2.5 mm) ATMA group was considered based on a bimodal distribution of measured ATMA.

Results

Mean Erun was 4.9 ± 0.4 J · kg−1 · m−1. The relationship between ATMA and Erun was not significant (r2 = 0.13, p = 0.12). Maximum AT force during stance was significantly lower in LONG (5,819 ± 1,202 N) compared to SHORT (6,990 ± 920 N, p = 0.028). Neither AT stretch nor AT strain energy storage was different between groups (mean difference: 0.3 ± 1 J · step−1, p = 0.84). Fascicle force was significantly higher in SHORT (508 ± 93 N) compared to LONG (468 ± 84 N. p = 0.02). Fascicle lengths and velocities were similar between groups (p > 0.72). Muscle energy cost was significantly lower in LONG (0.028 ± 0.08 J · kg · step−1) compared to SHORT (0.045 ± 0.14 J · kg · step−1p = 0.004). There was a significant negative relationship between ATMA and total muscle energy cost relative to body mass across the stance phase (r = −0.699, p < 0.001).

Discussion

Together these results suggest that a LONG ATMA serves to potentially reduce Erun by reducing the muscle energy cost of the plantarflexors during stance. The relative importance of AT energy storage and return in reducing Erun should be re-considered.
Dachas and food democracy—What makes a (good) food citizen?
Authors: Pungas Lilian
Published: April 2023

Against the backdrop of multiple crises within—and due to—the current industrial agri-food system, food is a highly political issue. As calls for food sovereignty grow louder and the war in Ukraine exposes the fragility of global food systems, the concept of food democracy calls on all (food) citizens to engage in a democratic and collective struggle for socially just and environmentally friendly food systems. To date, “Western” examples of food democracy and formal political procedures of civil society have dominated scholarship, ignoring the self-organized, low-key, and informal political activities around food in the post-socialist East. In this article, we shed light on the aspects of food democracy within Food Self-Provisioning (FSP) practices in Eastern Estonia, which is our case study. Our empirical data is based on semi-structured interviews conducted in 2019–2021 with 27 gardeners on their so-calleddachas—a Russian term for a plot of land with a seasonal allotment house used primarily for food production. The analysis focuses on the food-, farming-, and nutrition-related attitudes and practices of the gardeners, as well as the multitude of collective endeavors to improve food systems. Despite the precarious socio-economic and political status of the gardeners, we identified a variety of subtle, informal, and mundane forms of democratic practices and everyday resistance. We investigate the interplay of these aspects along the three dimensions of food democracy (input, throughput, output). On the one hand, FSP on Eastern Estonian dachas encompasses essential characteristics of the mainly “Western” concept of food democracy, allowing access to and participation in agricultural production while preserving (re)productive nature in the future. On the other hand, we caution against excessive optimism and romanticization of such local food communities, as they tend to remain exceptions and risk extinction or displacement if they are not valorized and reshaped through public discourse. We conclude with a plea for building and strengthening alliances between the marginalized elderly rural food producers and the more youthful urban food activists to achieve more democratic, just, and ecologically sound food systems.
Significance of collateral circulation in managing persistent sciatic artery: Two case reports
Authors: Yun Woo-Sung , Kim Hyeon Ju , Hwang Deokbi , Kim Hyung-Kee
Published: April 2023

Persistent sciatic artery (PSA) is a rare congenital anomaly considered an embryologic remnant of the internal iliac artery. Traditionally, the classification systems categorized PSA based on the completeness of PSA and superficial femoral artery (SFA) alongside the origin of PSA. The most common class has been known as type 2a in Pillet–Gauffre classification, meaning complete PSA with incomplete SFA. The mainstay of these patients with limb ischemia has been surgical bypass alongside excision or ligation of PSA aneurysm if present. However, the current PSA classification system does not account for collateral blood flow. Herein, we described two cases of type 2a PSA with distal embolization and explored therapeutic choices for PSA based on collateral presence. The first patient was treated with thromboembolectomy and patch angioplasty, and the second with conservative management. Despite distal embolization in both patients, bypass surgery was avoided, and distal circulation was maintained via collaterals from deep and superficial femoral arteries without increased risk of recurrent embolization. Thus, carefully examining collateral circulation and customized strategy is essential for managing PSA.
Fluorinated Derivatives of Digalloyl-Flavan-3-ol Induce Autophagic Cell Death by Forming Granular Aggregates Containing Mitochondria
Authors: Doge Ryo , Nishino Yuki , Saito Akiko
Published: April 2023

Flavan-3-ol derivatives are polyphenolic compounds with multifunctional properties. One of the flavan-3-ol derivatives, green tea catechin epigallocatechin gallate, is known to have anticancer activity as one of its multifunctional properties. We have studied the synthesis of flavan-3-ol derivatives and conducted structure-activity relationship studies; we found that the fluorinated derivatives exhibited high toxicity against HeLa and A549 cells. It was confirmed that the cytotoxicity was affected by the conformation of the flavan-3-ol skeleton and that the 2,3-cis form was dominant. The addition of fluorinated compounds increased the amount of intracellular mitochondrial superoxide, abolished the membrane potential of mitochondria, and, interestingly, formed granular aggregates containing mitochondria. When the level of LC3-II, a marker of autophagy induction, was confirmed, it suggested that the addition of the fluorinated compounds promoted autophagy. These results suggest that the novel highly cytotoxic fluorinated flavan-3-ol compound synthesized in this study promotes autophagy and induces cell death by triggering mitochondrial dysfunction. We believe that these results suggest the possibility of conferring more functionality through structural transformations of flavan-3-ol derivatives.
Biology-Informed Recurrent Neural Network for Pandemic Prediction Using Multimodal Data
Authors: Ding Zhiwei , Sha Feng , Zhang Yi , Yang Zhouwang
Published: April 2023

In the biomedical field, the time interval from infection to medical diagnosis is a random variable that obeys the log-normal distribution in general. Inspired by this biological law, we propose a novel back-projection infected–susceptible–infected-based long short-term memory (BPISI-LSTM) neural network for pandemic prediction. The multimodal data, including disease-related data and migration information, are used to model the impact of social contact on disease transmission. The proposed model not only predicts the number of confirmed cases, but also estimates the number of infected cases. We evaluate the proposed model on the COVID-19 datasets from India, Austria, and Indonesia. In terms of predicting the number of confirmed cases, our model outperforms the latest epidemiological modeling methods, such as vSIR, and intelligent algorithms, such as LSTM, for both short-term and long-term predictions, which shows the superiority of bio-inspired intelligent algorithms. In general, the use of mobility information improves the prediction accuracy of the model. Moreover, the number of infected cases in these three countries is also estimated, which is an unobservable but crucial indicator for the control of the pandemic.
Room Temperature Surfactant-Free Synthesis of Gold Nanoparticles in Alkaline Ethylene Glycol
Authors: Rasmussen Ditte Røjkjær , Nielsen Malthe Friis , Quinson Jonathan
Published: April 2023

Gold nanoparticles are easily obtained by a range of room temperature processes. In particular, polyols-based syntheses performed in alkaline conditions without the need for surfactants lead to small size nanoparticles around 10 nm in diameter. While highly viscous polyols, such as glycerol, have been the most studied polyols with which to perform the reaction, the use of alternative alcohols with lower viscosity could benefit the processing of the nanoparticles. Here, we show that ethylene glycol is a suitable alternative to glycerol. Via a study comprising more than 70 experiments overall, we identified that the key parameters by which to control nanoparticle size and colloidal stability are the amount of base used and the amount of ethylene glycol. Too-high or too-low values of base and/or ethylene glycol do not lead to stable colloidal nanoparticles. An optimal Base/Gold molar ratio is around 4 and an optimal amount of ethylene glycol is around 30 v.% to obtain stable ca. 10 nm Au NPs and to develop a green room temperature surfactant-free colloidal synthesis of gold nanoparticles.
Category Theory in Chemistry
Authors: Akitsu Takashiro
Published: April 2023

“Mathematical chemistry” as an academic field is said to have been proposed by Weyl in the 20th century as a way of thinking that abstracts and expresses “variables”, “symbols” and “functions” [...]
Macrophage-Induced Exacerbation of Nasopharyngeal Inflammatory Lymphocytes in COVID-19 Disease
Authors: Ayass Mohamad Ammar , Tripathi Trivendra , Griko Natalya , Ramankutty Nair Ramya , Zhang Jin , Zhu Kevin , Cao Wanying , Pashkov Victor , Okyay Tutku , Singh Sharda Kalla , Abi-Mosleh Lina
Published: April 2023

The nasal microenvironment plays a crucial role in the transmission, modulation, and clinical progression of COVID-19; however, the immune responses at the site of viral entry remain poorly understood. We deciphered the link between nasopharyngeal (NP) immune and inflammatory response that triggers cytokine/chemokine storms in the nasal route of COVID-19-positive patients. We used RT-PCR, multiplex ELISA, flow cytometry, and LC-MS/MS to decipher nasopharyngeal immune perturbations associated with severe COVID-19. In addition, we performed in vitro assays using cultured human monocytes-derived macrophages trained both in the presence and absence of SARS-CoV-2 trimeric spike protein(s) and co-cultured with and without autologous human peripheral blood mononuclear cells (hPBMCs)/total T-cells/CD8 T-cells. In vitro immune perturbations were examined by flow cytometry and LC-MS/MS assays. Our findings confirm that macrophages orchestrate NP immune inflammatory responses and highlight the cytokine/chemokine storms associated with the increased CD8+T-cells along with Tregs, Th1, and Th17.1 T-helper cells. We observed a correlation between in vitro and nasal findings that trained macrophages, profoundly M2c, differentially promote the inflammatory surfactome on CD8 T-cells, including ITGAM, LGALS3, CD38, TKT, LRPAP1, and SSBP1. The findings of this study conclude that inflammatory lymphocyte perturbations within the nasopharynx of COVID-19 patients may enforce immune homeostasis during SARS-CoV-2-infection and contribute to COVID-19 pathology. This study explored the therapeutic target proteins that could facilitate the development of new medications, which could allow for immediate treatment of possible emerging viral infections.
Motivation, Intention and Opportunity: Wearing Masks and the Spread of COVID-19
Authors: Kaine Geoff , Wright Vic
Published: April 2023

Prior to the emergence of the Omicron variant, we found large regional differences (ƞ2 = 0.19) in the frequency of wearing face masks in New Zealand even though the strength of people’s motivation to wear face masks to prevent the spread of COVID-19 was similar across regions. These differences were associated with regional differences (as measured by case numbers) in the risk of COVID-19 infection. The emergence of Omicron and its spread throughout New Zealand in conjunction with the cessation of lockdowns offered the opportunity to test whether regional differences in the frequency of mask wearing disappeared once the risk of COVID-19 infection became uniform across the country. It also created an opportunity to investigate differences in people’s behaviour with respect to wearing masks in private and in public. The results confirmed that regional differences in the frequency of mask wearing disappeared once Omicron spread through the country. We also found that the frequency of wearing masks was significantly lower when with family or friends than when out in public.
Analysis of Scratch Software in Scientific Production for 20 Years: Programming in Education to Develop Computational Thinking and STEAM Disciplines
Authors: Dúo-Terrón Pablo
Published: April 2023

Scratch is an educational software based on visual programming blocks. It was created in 2003 by the Massachusetts Institute of Technology Media Lab (MIT) and it develops computational thinking (CT) skills from an early age in schools and allows STEM (science, technology, engineering and mathematics) projects to be carried out. The aim of this research is to know the development of the scientific production of the Scratch programme in the educational field in scientific articles in WoS and its link with the STEM field. The methodology used in this study is of a bibliometric nature with an analysis of the development in the scientific literature and co-words. The Scratch in Education (Scratch-EDU) programme has been studied using the Web of Science (WoS) database. WoS, Vosviewer and SciMAT were used to extract the results and a total of 579 manuscripts were analysed. The results of the study show that the first scientific article on Scratch published in WoS dates back to 2004, although it is from 2011 when a considerable volume of studies began to appear in the scientific literature, and moreover, in recent years the scientific literature relates Scratch-EDU with topics and keywords related to the STEM field. The conclusions of the study are that the Scratch programme has had a progressive evolution in the scientific field related to education from 2012 to 2020, mainly in proceedings papers, with a decrease in manuscripts in the last two years. The emerging themes and keywords that have most influenced Scratch-EDU manuscripts in recent years are related to the terms “Implementation” and “Curriculum”, connected in turn, with terms such as “pedagogy”, “public school” or “students”. Another term that stands out in the development of scientific evolution is “Computational Thinking”, associated with topics such as “Primary Education”, “Learning” or “Problem Solving”. Finally, a discussion and conclusion of the results has been carried out, which can serve as a turning point for future lines of research on programming and CT in the STEM field from an early age in education.
Transit Behaviour and Sociodemographic Interrelation: Enhancing Urban Public-Transport Solutions
Authors: Hasan Aisha , Hasan Umair , AlJassmi Hamad , Whyte Andrew
Published: April 2023

Recent years have seen a considerable shift in the focus of public investment agencies from extensive roadway networks to a more planned approach that meets environmental, cost, and social dimensions more aptly. Past research has mainly explored the engineering aspect and cost parameters, while the human or social component is often neglected. This study aims to identify the trip-making behaviour of residents in an urban area towards bus transport network enhancement. Abu Dhabi, the location of study, is heavily dependent upon car travel, creating much congestion, which the local government seeks to address by enhanced public transport. This work examined eight public-transport routes in two zones, with data collected on both weekdays (n = 751) and weekends (n = 769). Multinomial logistic regression models showed that respondents highlighted overcrowded buses and traffic congestion as two of the main hurdles pertinent to urban routes in the bus network influencing their mode choice. Proposals pertinent to the local authority for further consideration need to factor in current low satisfaction with bus transit network coverage, low satisfaction with the quality of bus rides, inhibiting a mode shift from cars/taxis towards buses, cumulative income profiles of public-transport users, with findings that the low-income bracket is already at saturation, and that reducing congestion needs innovative (sociodynamic rather than technical road network) public-transport solutions.
Effect of Sample Preparation on Saturated and Unsaturated Shear Strength of Cohesionless Soils
Authors: Akram Ilyas , Azam Shahid
Published: April 2023

The geotechnical behavior of cohesionless soils is governed by field conditions. Such soils exist in two distinct forms, namely: disintegrated, such as fresh sediments under no overburden and/or no suction, and intact, such as old deposits with overburden and/or suction. The main contribution of this research was the successful capture of field conditions in laboratory samples, and the determination of shear strength under saturated and dried states. Results indicated that disintegrated samples possess identical soil behavior under both saturation states. Shear stiffness and peak shear increased with increasing normal stress, and no clear failure peaks were observed, similar to loose soils. Both samples showed an initial contraction followed by dilation at low normal stresses and mostly contraction at high normal stresses. Apparent cohesion was non-existent, and the friction angle measured 44.5° in the saturated state and 48° in the dried state. The intact sample exhibited behavior similar to the disintegrated sample when saturated. Under the dried state, clear failure peaks followed by residual shear were observed, similar to dense soils. Soil response was primarily dilative at low normal stresses and largely contractive under high normal stresses. Apparent cohesion was zero, and friction angle was 42° in the saturated state and changed to 91 kPa and 36°, respectively, in the dried state. Finally, structural cohesion increased with normal stress, and the friction angle due to suction was between 0.05° and 0.02°.
Water Heritage in the Rural Hinterland Landscapes of the UNESCO Alto Douro Wine Region, Portugal: A Digital Humanities Approach
Authors: Vidal Gonçalves Gerardo , Borges Pereira Dina , Gómez-Ullate Martín , da Silva Mano André
Published: April 2023

Starting from a well defined and demarcated area in space, the Corgo River, in the region of Trás-os-Montes and Alto Douro, this work seeks through Information Technologies (IT), Digital Humanities and online tools and applications (software) to provide digital images of space and water resources, anthropic elements related to water and various natural features. Based on these available resources and a set of historical cartographic documents, we provide a realistic view of the cultural and natural water landscape and how augmented reality can help archaeology interpret this landscape and its historical transformations.
Tracking Trajectories: Projecting Polychromy onto a Roman Relief from a Scottish Castle
Authors: Campbell Louisa
Published: April 2023

The Antonine Wall Distance Sculptures are iconic and unique sculptural reliefs that marked Rome’s most north-westerly frontier across central Scotland. Their inscribed texts and iconography depict graphic tales of frontier life, and recent non-invasive analysis has confirmed they were originally brought to life through vibrant polychromy. This paper tracks the trajectory of one Distance Sculpture that was embedded into the dramatic setting of Dunnottar Castle off the north-east coast of Scotland during the 16th century, where it was recorded as having been repainted during that episode of use. A suite of complementary analytical techniques, including pXRF, FTIR, and SEM/EDS, was recently reported on which identified pigments and surface treatments as well as their chronology of application, confirmed through stratigraphic sequencing visible in cross-section. That approach facilitated the investigation of all episodes in the itinerary of this iconic sculpted relief from the second century to the Scottish Renaissance. That vanguard research has provided an unprecedented opportunity to unravel the rich hidden history behind this unique monumental inscription and re-tell a fascinating transformational tale of a pivotal period in its past. The combination of historical, archaeological, and scientific approaches to an understudied, and overlooked, phenomenon of post-antique colouration is revolutionary in polychromy studies. It provides innovative and well-contextualised information that lifts an aesthetically modest Roman monument into a vibrant, colourful, and sumptuous decorative feature fit to grace the walls of a Renaissance castle emulating Roman imperial practices. We can now trace its journey through time by delving into the detail of its Renaissance repainting to present, for the first time, an accurate digital reconstruction as it performed for 16th century audiences.
Life History Strategy of Maurolicus muelleri (Gmenlin, 1789) in the Bay of Biscay
Authors: Alvarez Paula , Korta Maria , Garcia Dorleta , Boyra Guillermo
Published: April 2023

Maurolicus muelleri is a significant component of the marine ecosystem and has the potential to be a valuable fishery resource. However, in the Bay of Biscay, its primary biological traits remain unclear. This study presents data on the length distribution, age, growth, maturity ogive, spawning season, batch fecundity, and sex ratio for Maurolicus muelleri captured in the Bay of Biscay. The results showed that in spring, the adult spawners (ages of 1 and 2) were dominant in the catches, while in September, immature juveniles (age of 0) born in spring were mostly found. Using standard lengths as a basis, 50% of the fish were mature at 34.1 mm (both sexes combined), and the sex ratio, male to female, was 0.44:0.56. The proportion of females increased with length, and a 1:1 sex ratio was predicted at a standard length of 41.5 mm. The spawning season was allocated to at least between March and September, with a likely peak in May. The batch fecundity ranged from 114 to 919 oocytes/female, and increased with the weight and length of females. The results allowed us to interpret a life history strategy for this species, i.e., a high fecundity for females, which mostly participate in one or two reproductive seasons. Therefore, any possible exploitation of age 0 fish prior to spawning could lead to a decrease in the population from which recovery could be slow.
Ursodeoxycholic Acid Binds PERK and Ameliorates Neurite Atrophy in a Cellular Model of GM2 Gangliosidosis
Authors: Morales Carolina , Fernandez Macarena , Ferrer Rodrigo , Raimunda Daniel , Carrer Dolores C. , Bollo Mariana
Published: April 2023

The Unfolded protein response (UPR), triggered by stress in the endoplasmic reticulum (ER), is a key driver of neurodegenerative diseases. GM2 gangliosidosis, which includes Tay-Sachs and Sandhoff disease, is caused by an accumulation of GM2, mainly in the brain, that leads to progressive neurodegeneration. Previously, we demonstrated in a cellular model of GM2 gangliosidosis that PERK, a UPR sensor, contributes to neuronal death. There is currently no approved treatment for these disorders. Chemical chaperones, such as ursodeoxycholic acid (UDCA), have been found to alleviate ER stress in cell and animal models. UDCA’s ability to move across the blood-brain barrier makes it interesting as a therapeutic tool. Here, we found that UDCA significantly diminished the neurite atrophy induced by GM2 accumulation in primary neuron cultures. It also decreased the up-regulation of pro-apoptotic CHOP, a downstream PERK-signaling component. To explore its potential mechanisms of action, in vitro kinase assays and crosslinking experiments were performed with different variants of recombinant protein PERK, either in solution or in reconstituted liposomes. The results suggest a direct interaction between UDCA and the cytosolic domain of PERK, which promotes kinase phosphorylation and dimerization.
Nitric Oxide Induced by Ammonium/Nitrate Ratio Ameliorates Low-Light Stress in Brassica pekinesis: Regulation of Photosynthesis and Root Architecture
Authors: Hu Linli , Gao Xueqin , Li Yutong , Lyu Jian , Xiao Xuemei , Zhang Guobin , Yu Jihua
Published: April 2023

Low-light intensity affects plant growth and development and, finally, causes a decrease in yield and quality. There is a need for improved cropping strategies to solve the problem. We previously demonstrated that moderate ammonium:nitrate ratio (NH4+:NO3−) mitigated the adverse effect caused by low-light stress, although the mechanism behind this alleviation is unclear. The hypothesis that the synthesis of nitric oxide (NO) induced by moderate NH4+:NO3− (10:90) involved in regulating photosynthesis and root architecture of Brassica pekinesis subjected to low-light intensity was proposed. To prove the hypothesis, a number of hydroponic experiments were conducted. The results showed that in plants exposed to low-light intensity, the exogenous donors NO (SNP) and NH4+:NO3− (N, 10:90) treatments significantly increased leaf area, growth range, and root fresh weight compared with nitrate treatment. However, the application of hemoglobin (Hb, NO scavenger), N-nitro-l-arginine methyl ester (L-NAME, NOS inhibitor), and sodium azide (NaN3, NR inhibitor) in N solution remarkably decreased the leaf area, canopy spread, the biomass of shoot and root, the surface area, and volume and tips of the root. The application of N solution and exogenous SNP significantly enhanced Pn (Net photosynthetic rate) and rETR (relative electron transport rates) compared with solo nitrate. While all these effects of N and SNP on photosynthesis, such as Pn, Fv/Fm (maximum quantum yield of PSII), Y(II) (actual photosynthetic efficiency), qP (photochemical quenching), and rETR were reversed when the application of Hb, L-NAME, and NaN3 in N solution. The results also showed that the N and SNP treatments were more conducive to maintaining cell morphology, chloroplast structure, and a higher degree of grana stacking of low-light treated plants. Moreover, the application of N significantly increased the NOS and NR activities, and the NO levels in the leaves and roots of mini Chinese cabbage seedlings treated with N were significantly higher than those in nitrate-treated plants. In conclusion, the results of this study showed that NO synthesis induced by the appropriate ammonia–nitrate ratio (NH4+: NO3− = 10:90) was involved in the regulation of photosynthesis and root structure of Brassica pekinesis under low-light stress, effectively alleviating low-light stress and contributing to the growth of mini Chinese cabbage under low-light stress.
Reoperation in Chiari-1 Malformations
Authors: Talamonti Giuseppe , Picano Marco , Fragale Maria , Marcati Eleonora , Meccariello Giulia , Boeris Davide , Cenzato Marco
Published: April 2023

(1) Background: The issue of unsuccessful surgery for Chiari-1 malformation (CM-1), as well as its potential causes and possible solutions, remains poorly documented and studied. (2) Methods: From a retrospective review of a personal series of 98 patients undergoing treatment for CM-1 during the past 10 years, we created two study groups. Group 1: 8 patients (8.1%) requiring additional surgeries owing to postoperative complications (7 cerebrospinal fluid leakage, 1 extradural hematoma); 7 patients (7.1%) undergoing reoperations for failed decompression during the follow-up. Group 2: During the same period, we also managed 19 patients who had previously been operated on elsewhere: 8 patients who required adequate CM-1 treatment following extradural section of the filum terminale; 11 patients requiring reoperations for failed decompression. Failed decompression was managed by adequate osteodural decompression, which was associated with tonsillectomy (6 cases), subarachnoid exploration (8 cases), graft substitution (6 cases), and occipito-cervical fixation/revision (1 case). (3) Results: There was no mortality or surgical morbidity in Group 1. However, one patient’s condition worsened due to untreatable syrinx. In Group 2, there were two cases of mortality, and surgical morbidity was represented by functional limitation and pain in the patient who needed revision of the occipitocervical fixation. Twenty patients improved (58.8%), 6 remained unchanged (32.3%), 1 worsened (2.9%) and 2 died (5.9%). (4) Conclusions: The rate of complications remains high in CM-1 treatment. Unfortunately, a certain rate of treatment failure is unavoidable, but it appears that a significant number of re-operations could have been avoided using proper indications and careful technique.
Study on the Accessibility Impact of Anti-Rolling Tank on the Offshore Wind O&M Gangway
Authors: Zhang Songtao , Zhao Peng , Li Chenyang , Song Ziqi , Liang Lihua
Published: April 2023

In order to improve the accessibility of offshore wind O&M gangway in complex sea conditions, a control method for the controllable anti-rolling tank based on wavelet neural network period prediction is proposed in this paper. Firstly, a model of ship motion with a controllable passive anti-rolling tank was established. Then, the inverse kinematics of the gangway were carried out. In order to analyze the influence of the anti-rolling tank on the accessibility of the on-board gangway, an accessibility simulation of the gangway under different sea conditions was completed. Finally, a hardware-in-the-loop simulation of the active compensation gangway system was carried out. The simulation and test results show that the anti-rolling tank can effectively restrain the rolling motion of the ship and significantly improve the accessibility of the on-board gangway.
Systematic Investigation on Supported Gold Catalysts Prepared by Fluorine-Free Basic Etching Ti3AlC2 in Selective Oxidation of Aromatic Alcohols to Aldehydes
Authors: Jiang Hangwei , Chen Xiya , Cui Danlan , Lu Kun , Kong Xiao , Zhang Xingguang
Published: April 2023

Conventional methods to prepare supported metal catalysts are chemical reduction and wet impregnation. This study developed and systematically investigated a novel reduction method based on simultaneous Ti3AlC2 fluorine-free etching and metal deposition to prepare gold catalysts. The new series of Aupre/Ti3AlxC2Ty catalysts were characterized by XRD, XPS, TEM, and SEM and were tested in the selective oxidation of representative aromatic alcohols to aldehydes. The catalytic results demonstrate the effectiveness of the preparation method and better catalytic performances of Aupre/Ti3AlxC2Ty, compared with those of catalysts prepared by traditional methods. Moreover, this work presents a comprehensive study on the influence of calcination in air, H2, and Ar, and we found that the catalyst of Aupre/Ti3AlxC2Ty-Air600 obtained by calcination in air at 600 °C performed the best, owing to the synergistic effect between tiny surface TiO2 species and Au NPs. The tests of reusability and hot filtration confirmed the catalyst stability.
Accuracy of Therapeutic Drug Monitoring of Teicoplanin at the Onset of Febrile Neutropenia
Authors: Takigawa Masaki , Tanaka Hiroyuki , Suwa Junichi , Obara Tomoya , Maeda Yohei , Sato Mamoru , Shimazaki Yoshitomo , Onoda Toshihisa , Ishigami Akihito , Ishii Toshihiro
Published: April 2023

Background and Objectives: Teicoplanin (TEIC) is an effective drug for patients with febrile neutropenia (FN); however, it has been reported that these patients may have increased TEIC clearance compared with patients who do not have FN. The purpose of this study was to study therapeutic drug monitoring in patients with FN when the TEIC dosing design was based on the population mean method. Materials and Methods: Thirty-nine FN patients with hematological malignancy were included in the study. To calculate the predicted blood concentration of TEIC, we used the two population pharmacokinetic (population PK) parameters (parameters 1 and 2) reported by Nakayama et al. and parameter 3, which is a modification of the population PK of Nakayama et al. We calculated the mean prediction error (ME), an indicator of prediction bias, and the mean absolute prediction error (MAE), an indicator of accuracy. Furthermore, the percentage of predicted TEIC blood concentration within 25% and 50% of the measured TEIC blood concentration was calculated. Results: The ME values were −0.54, −0.25, and −0.30 and the MAE values were 2.29, 2.19, and 2.22 for parameters 1, 2, and 3, respectively. For all of the three parameters, the ME values were calculated as minus values, and the predicted concentrations tended to be biased toward smaller values relative to the measured concentrations. Patients with serum creatinine (Scr) < 0.6 mg/dL and neutrophil counts < 100/μL had greater ME and MAE values and a smaller percentage of predicted TEIC blood concentration within 25% of measured TEIC blood concentrations compared with other patients. Conclusions: In patients with FN, the accuracy of predicting TEIC blood concentrations was good, with no significant differences between each parameter. However, patients with a Scr < 0.6 mg/dL and a neutrophil count < 100/μL showed slightly inferior prediction accuracy.
The Association between Socio-Demographic Characteristics and Using Pain Assessment Tools among Critically Ill Patients
Authors: Rababa Mohammad , Al-Sabbah Shatha , Eyadat Anwar M. , Abusbaitan Hanan A.
Published: April 2023

Background and Objectives: Pain is still undertreated among ICU patients, especially cognitively impaired patients. Nurses play a crucial role in their management. However, previous studies found that nurses had insufficient knowledge about pain assessment and management. Some nurses’ socio-demographic characteristics, such as being female; age; years of experience; type of unit, either medical or surgical; education level; years of nursing experience; qualification; position; and hospital level, were found to be associated with their practices of pain assessment and management. This study aimed to examine the association between nurses’ socio-demographic characteristics and the use of pain assessment tools for critically ill patients. Materials and Methods: A convenience sample of 200 Jordanian nurses responded to the Pain Assessment and Management for the Critically Ill questionnaire to achieve the study’s aim. Results: The type of hospital, academic qualification, years of experience as a critical care nurse, and hospital affiliation were significantly associated with increased use of self-report pain assessment tools for verbal patients, while the type of hospital and hospital affiliation was significantly associated with an increased use of observational pain assessment tools for nonverbal patients. Conclusion: Examining the association between socio-demographic characteristics and the use of pain assessment tools for critically ill patients is essential for quality pain practice.
Treatment of Cesarean Scar and Cervical Pregnancies Using the Ovum Aspiration Set for Intrachorial Methotrexate Injection as a Conservative, Fertility-Preserving Procedure
Authors: Ge Isabell , Geißler Carmen , Geffroy Alexandra , Juhasz-Böss Ingolf , Wiehle Philipp , Asberger Jasmin
Published: April 2023

Background and Objectives: Cesarean scar and cervical pregnancies are rare forms of ectopic pregnancies, occurring in 1 in 2000 and 1 in 9000 pregnancies, respectively. Both entities are medically challenging due to their high morbidity and mortality potential. Materials and Methods: In this retrospective study, we analyzed all cesarean scar and cervical pregnancies from 2010 to 2019 in the Department of Gynecology and Obstetrics of the University Hospital Freiburg, treated with both intrachorial (using the ovum aspiration set) and systemic methotrexate application. Results: We identified seven patients with a cesarean scar and four patients with cervical pregnancies. At diagnosis, the median gestational age was 7 + 1 (range: 5 + 5–9 + 5) weeks and the mean value of ß-hCG was 43,536 (range: 5132–87842) mlU/mL. On average, one dose of intrachorial and two doses of systemic methotrexate were administered per patient. The efficacy rate was 72.7% with three patients (27.3%) needing an additional surgical or interventional procedure. The uterus was preserved in 100% of the patients. Out of the eight patients with follow-up data, five reported subsequent pregnancies (62.5%) that resulted in six live births. None had recurrent cesarean scars or cervical pregnancies. In the subgroup analyses, when comparing cesarean scar pregnancies to cervical pregnancies, patient characteristics, treatment modality, and the outcome did not differ significantly, except for parity (2 versus 0, p = 0.02) and the duration since the last pregnancy (3 vs. 0.75 years, p = 0.048). When comparing cases with successful and failed methotrexate-only treatments, the maternal age was significantly higher in the successful group (34 vs. 27 years, p = 0.02). Localization of the gestation, gestational and maternal age, ß-hCG, and history of preceding pregnancies were non-predictive for the efficacy of the treatment. Conclusions: The combined application of intrachorial and systemic methotrexate for the treatment of cesarean scar and cervical pregnancies has been proven effective, well-tolerated, organ- and fertility-conserving with a low complication rate.
Assessment of Cu(II) Removal from Aqueous Solutions by Modified Pomelo Peels: Experiments and Modelling
Authors: Zhang Ruixue , Jiao Mengqing , Zhao Nan , Jacquemin Johan , Zhang Yinqin , Liu Honglai
Published: April 2023

In this study, low-cost pomelo peel wastes were used as a bio-sorbent to remove copper ions (e.g., Cu(II)) from aqueous solutions. Prior to testing its Cu(II) removal capability, the structural, physical and chemical characteristics of the sorbent were examined by scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, and Brunauer–Emmett–Teller (BET) surface area analysis. The impacts of the initial pH, temperature, contact time and Cu(II) feed concentration on the Cu(II) biosorption using modified pomelo peels were then assessed. Thermodynamic parameters associated to the biosorption clearly demonstrate that this biosorption is thermodynamically feasible, endothermic, spontaneous and entropy driven. Furthermore, adsorption kinetic data were found to fit very well with the pseudo-second order kinetics equation, highlighting that this process is driven by a chemical adsorption. Finally, an artificial neural network with a 4:9:1 structure was then established for describing the Cu(II) adsorption using modified pomelo peels with R2 values close to 0.9999 and to 0.9988 for the training and testing sets, respectively. The results present a big potential use of the as-prepared bio-sorbent for the removal of Cu(II), as well as an efficient green technology for ecological and environmental sustainability.
Novel Functionalized Boron Nitride Nanosheets Achieved by Radiation-Induced Oxygen Radicals and Their Enhancement for Polymer Nanocomposites
Authors: Yang Xin , Zhao Bingling , Ji Liudi , Hu Peng , Zhu Xiaoming , Li Zeyu
Published: April 2023

Boron nitride nanosheets (BNNSs) exfoliated from hexagonal boron nitride (h-BN) show great potential in polymer-based composites due to their excellent mechanical properties, highly thermal conductivity, and insulation properties. Moreover, the structural optimization, especially the surface hydroxylation, of BNNSs is of importance to promote their reinforcements and optimize the compatibility of its polymer matrix. In this work, BNNSs were successfully attracted by oxygen radicals decomposed from di-tert-butylperoxide (TBP) induced by electron beam irradiation and then treated with piranha solution. The structural changes of BNNSs in the modification process were deeply studied, and the results demonstrate that the as-prepared covalently functionalized BNNSs possess abundant surface hydroxyl groups as well as reliable structural integrity. Of particular importance is that the yield rate of the hydroxyl groups is impressive, whereas the usage of organic peroxide and reaction time is greatly reduced due to the positive effect of the electron beam irradiation. The comparisons of PVA/BNNSs nanocomposites further indicate that the hydroxyl-functionalized BNNSs effectively promote mechanical properties and breakdown strength due to the enhanced compatibility and strong two-phase interactions between nanofillers and the polymer matrix, which further verify the application prospects of the novel route proposed in this work.
Structural Analysis of Spermidine Synthase from Kluyveromyces lactis
Authors: Kim Seongjin , Chang Jeong Ho
Published: April 2023

Spermidine is a polyamine molecule that performs various cellular functions, such as DNA and RNA stabilization, autophagy modulation, and eIF5A formation, and is generated from putrescine by aminopropyltransferase spermidine synthase (SpdS). During synthesis, the aminopropyl moiety is donated from decarboxylated S-adenosylmethionine to form putrescine, with 5′-deoxy-5′-methylthioadenosine being produced as a byproduct. Although the molecular mechanism of SpdS function has been well-established, its structure-based evolutionary relationships remain to be fully understood. Moreover, only a few structural studies have been conducted on SpdS from fungal species. Here, we determined the crystal structure of an apo-form of SpdS from Kluyveromyces lactis (KlSpdS) at 1.9 Å resolution. Structural comparison with its homologs revealed a conformational change in the α6 helix linked to the gate-keeping loop, with approximately 40° outward rotation. This change caused the catalytic residue Asp170 to move outward, possibly due to the absence of a ligand in the active site. These findings improve our understanding of the structural diversity of SpdS and provide a missing link that expands our knowledge of the structural features of SpdS in fungal species.