bluuebunny/crossref_metadata_2025 huggingface dataset:
The development of brain pericytes requires expression of the transcription factor nkx3.1 in intermediate precursors
Authors: Ahuja Suchit , Adjekukor Cynthia , Li Qing , Kocha Katrinka M. , Rosin Nicole , Labit Elodie , Sinha Sarthak , Narang Ankita , Long Quan , Biernaskie Jeff , Huang Peng , Childs Sarah J.
Published: April 2024

Brain pericytes are one of the critical cell types that regulate endothelial barrier function and activity, thus ensuring adequate blood flow to the brain. The genetic pathways guiding undifferentiated cells into mature pericytes are not well understood. We show here that pericyte precursor populations from both neural crest and head mesoderm of zebrafish express the transcription factor nkx3.1 develop into brain pericytes. We identify the gene signature of these precursors and show that an nkx3.1-, foxf2a-, and cxcl12b-expressing pericyte precursor population is present around the basilar artery prior to artery formation and pericyte recruitment. The precursors later spread throughout the brain and differentiate to express canonical pericyte markers. Cxcl12b-Cxcr4 signaling is required for pericyte attachment and differentiation. Further, both nkx3.1 and cxcl12b are necessary and sufficient in regulating pericyte number as loss inhibits and gain increases pericyte number. Through genetic experiments, we have defined a precursor population for brain pericytes and identified genes critical for their differentiation.
Direct Salmonella injection into enteroid cells allows the study of host–pathogen interactions in the cytosol with high spatiotemporal resolution
Authors: Ernst Chantal , Andreassen Patrick R. , Giger Gabriel H. , Nguyen Bidong D. , Gäbelein Christoph G. , Guillaume-Gentil Orane , Fattinger Stefan A. , Sellin Mikael E. , Hardt Wolf-Dietrich , Vorholt Julia A.
Published: April 2024

Intestinal epithelial cells (IECs) play pivotal roles in nutrient uptake and in the protection against gut microorganisms. However, certain enteric pathogens, such as Salmonella enterica serovar Typhimurium (S. Tm), can invade IECs by employing flagella and type III secretion systems (T3SSs) with cognate effector proteins and exploit IECs as a replicative niche. Detection of flagella or T3SS proteins by IECs results in rapid host cell responses, i.e., the activation of inflammasomes. Here, we introduce a single-cell manipulation technology based on fluidic force microscopy (FluidFM) that enables direct bacteria delivery into the cytosol of single IECs within a murine enteroid monolayer. This approach allows to specifically study pathogen–host cell interactions in the cytosol uncoupled from preceding events such as docking, initiation of uptake, or vacuole escape. Consistent with current understanding, we show using a live-cell inflammasome reporter that exposure of the IEC cytosol to S. Tm induces NAIP/NLRC4 inflammasomes via its known ligands flagellin and T3SS rod and needle. Injected S. Tm mutants devoid of these invasion-relevant ligands were able to grow in the cytosol of IECs despite the absence of T3SS functions, suggesting that, in the absence of NAIP/NLRC4 inflammasome activation and the ensuing cell death, no effector-mediated host cell manipulation is required to render the epithelial cytosol growth-permissive for S. Tm. Overall, the experimental system to introduce S. Tm into single enteroid cells enables investigations into the molecular basis governing host–pathogen interactions in the cytosol with high spatiotemporal resolution.
Learning spatio-temporal patterns with Neural Cellular Automata
Authors: Richardson Alex D. , Antal Tibor , Blythe Richard A. , Schumacher Linus J.
Published: April 2024

Neural Cellular Automata (NCA) are a powerful combination of machine learning and mechanistic modelling. We train NCA to learn complex dynamics from time series of images and Partial Differential Equation (PDE) trajectories. Our method is designed to identify underlying local rules that govern large scale dynamic emergent behaviours. Previous work on NCA focuses on learning rules that give stationary emergent structures. We extend NCA to capture both transient and stable structures within the same system, as well as learning rules that capture the dynamics of Turing pattern formation in nonlinear PDEs. We demonstrate that NCA can generalise very well beyond their PDE training data, we show how to constrain NCA to respect given symmetries, and we explore the effects of associated hyperparameters on model performance and stability. Being able to learn arbitrary dynamics gives NCA great potential as a data driven modelling framework, especially for modelling biological pattern formation.
Informing policy via dynamic models: Cholera in Haiti
Authors: Wheeler Jesse , Rosengart AnnaElaine , Jiang Zhuoxun , Tan Kevin , Treutle Noah , Ionides Edward L.
Published: April 2024

Public health decisions must be made about when and how to implement interventions to control an infectious disease epidemic. These decisions should be informed by data on the epidemic as well as current understanding about the transmission dynamics. Such decisions can be posed as statistical questions about scientifically motivated dynamic models. Thus, we encounter the methodological task of building credible, data-informed decisions based on stochastic, partially observed, nonlinear dynamic models. This necessitates addressing the tradeoff between biological fidelity and model simplicity, and the reality of misspecification for models at all levels of complexity. We assess current methodological approaches to these issues via a case study of the 2010-2019 cholera epidemic in Haiti. We consider three dynamic models developed by expert teams to advise on vaccination policies. We evaluate previous methods used for fitting these models, and we demonstrate modified data analysis strategies leading to improved statistical fit. Specifically, we present approaches for diagnosing model misspecification and the consequent development of improved models. Additionally, we demonstrate the utility of recent advances in likelihood maximization for high-dimensional nonlinear dynamic models, enabling likelihood-based inference for spatiotemporal incidence data using this class of models. Our workflow is reproducible and extendable, facilitating future investigations of this disease system.
Human decision making balances reward maximization and policy compression
Authors: Lai Lucy , Gershman Samuel J.
Published: April 2024

Policy compression is a computational framework that describes how capacity-limited agents trade reward for simpler action policies to reduce cognitive cost. In this study, we present behavioral evidence that humans prefer simpler policies, as predicted by a capacity-limited reinforcement learning model. Across a set of tasks, we find that people exploit structure in the relationships between states, actions, and rewards to “compress” their policies. In particular, compressed policies are systematically biased towards actions with high marginal probability, thereby discarding some state information. This bias is greater when there is redundancy in the reward-maximizing action policy across states, and increases with memory load. These results could not be explained qualitatively or quantitatively by models that did not make use of policy compression under a capacity limit. We also confirmed the prediction that time pressure should further reduce policy complexity and increase action bias, based on the hypothesis that actions are selected via time-dependent decoding of a compressed code. These findings contribute to a deeper understanding of how humans adapt their decision-making strategies under cognitive resource constraints.
Conserved and novel enhancers in the Aedes aegypti single-minded locus recapitulate embryonic ventral midline gene expression
Authors: Schember Isabella , Reid William , Sterling-Lentsch Geyenna , Halfon Marc S.
Published: April 2024

Transcriptional cis-regulatory modules, e.g., enhancers, control the time and location of metazoan gene expression. While changes in enhancers can provide a powerful force for evolution, there is also significant deep conservation of enhancers for developmentally important genes, with function and sequence characteristics maintained over hundreds of millions of years of divergence. Not well understood, however, is how the overall regulatory composition of a locus evolves, with important outstanding questions such as how many enhancers are conserved vs. novel, and to what extent are the locations of conserved enhancers within a locus maintained? We begin here to address these questions with a comparison of the respective single-minded (sim) loci in the two dipteran species Drosophila melanogaster (fruit fly) and Aedes aegypti (mosquito). sim encodes a highly conserved transcription factor that mediates development of the arthropod embryonic ventral midline. We identify two enhancers in the A. aegypti sim locus and demonstrate that they function equivalently in both transgenic flies and transgenic mosquitoes. One A. aegypti enhancer is highly similar to known Drosophila counterparts in its activity, location, and autoregulatory capability. The other differs from any known Drosophila sim enhancers with a novel location, failure to autoregulate, and regulation of expression in a unique subset of midline cells. Our results suggest that the conserved pattern of sim expression in the two species is the result of both conserved and novel regulatory sequences. Further examination of this locus will help to illuminate how the overall regulatory landscape of a conserved developmental gene evolves.
Correlation-based tests for the formal comparison of polygenic scores in multiple populations
Authors: Gunn Sophia , Lunetta Kathryn L.
Published: April 2024

Polygenic scores (PGS) are measures of genetic risk, derived from the results of genome wide association studies (GWAS). Previous work has proposed the coefficient of determination (R2) as an appropriate measure by which to compare PGS performance in a validation dataset. Here we propose correlation-based methods for evaluating PGS performance by adapting previous work which produced a statistical framework and robust test statistics for the comparison of multiple correlation measures in multiple populations. This flexible framework can be extended to a wider variety of hypothesis tests than currently available methods. We assess our proposed method in simulation and demonstrate its utility with two examples, assessing previously developed PGS for low-density lipoprotein cholesterol and height in multiple populations in the All of Us cohort. Finally, we provide an R package ‘coranova’ with both parametric and nonparametric implementations of the described methods.
Deep mutational scanning of Pneumocystis jirovecii dihydrofolate reductase reveals allosteric mechanism of resistance to an antifolate
Authors: Rouleau Francois D. , Dubé Alexandre K. , Gagnon-Arsenault Isabelle , Dibyachintan Soham , Pageau Alicia , Després Philippe C. , Lagüe Patrick , Landry Christian R.
Published: April 2024

Pneumocystis jirovecii is a fungal pathogen that causes pneumocystis pneumonia, a disease that mainly affects immunocompromised individuals. This fungus has historically been hard to study because of our inability to grow it in vitro. One of the main drug targets in P. jirovecii is its dihydrofolate reductase (PjDHFR). Here, by using functional complementation of the baker’s yeast ortholog, we show that PjDHFR can be inhibited by the antifolate methotrexate in a dose-dependent manner. Using deep mutational scanning of PjDHFR, we identify mutations conferring resistance to methotrexate. Thirty-one sites spanning the protein have at least one mutation that leads to resistance, for a total of 355 high-confidence resistance mutations. Most resistance-inducing mutations are found inside the active site, and many are structurally equivalent to mutations known to lead to resistance to different antifolates in other organisms. Some sites show specific resistance mutations, where only a single substitution confers resistance, whereas others are more permissive, as several substitutions at these sites confer resistance. Surprisingly, one of the permissive sites (F199) is without direct contact to either ligand or cofactor, suggesting that it acts through an allosteric mechanism. Modeling changes in binding energy between F199 mutants and drug shows that most mutations destabilize interactions between the protein and the drug. This evidence points towards a more important role of this position in resistance than previously estimated and highlights potential unknown allosteric mechanisms of resistance to antifolate in DHFRs. Our results offer unprecedented resources for the interpretation of mutation effects in the main drug target of an uncultivable fungal pathogen.
Phenotypic and functional characteristics of monocyte subsets in the blood and bone marrow of Indian subjects with Visceral Leishmaniasis
Authors: Kausar Gulafsha , Chauhan Shashi Bhushan , Roy Ritirupa , Verma Vimal , Pandey Sundaram , Niyaz Aziza , Chakravarty Jaya , Engwerda Christian R. , Nylen Susanne , Kumar Rajiv , Wilson Mary E. , Sundar Shyam
Published: April 2024

Visceral leishmaniasis (VL) is a potentially fatal parasitic infection caused by Leishmania donovani in India. L. donovani is an obligate intracellular protozoan residing mostly in macrophages of the reticuloendothelial system throughout chronic infection. Monocytic phagocytes are critical in the pathogenesis of different forms of leishmaniasis. Subsets of monocytes are distinguished by their surface markers into CD14+CD16- classical monocytes, CD14+CD16+ intermediate monocytes, and CD16++CD14low non-classical monocyte subsets. During cutaneous leishmaniasis (CL), intermediate monocyte are reported to be a source of inflammatory cytokines IL-1β and TNF, and they express CCR2 attracting them to sites of inflammatory pathology. We examined monocyte subsets in the blood and bone marrow of patients with VL from an endemic site in Bihar, India, and found these contrasted with the roles of monocytes in CL. During VL, intermediate and non-classical CD16+ monocyte subsets expressed instead a non-inflammatory phenotype with low CCR2, high CX3CR1 and low microbicidal oxidant generation, making them more similar to patrolling monocytes than inflammatory cells. Bone marrow CD16+ monocyte subsets expressed a phenotype that might be more similar to the inflammatory subsets of CL, although our inability to obtain bone marrow from healthy donors in the endemic region hampered this interpretation Overall the data suggest that CD16+ intermediate monocyte subsets in VL patients express a phenotypes that contributes to an immunosuppressed pathologic immune state, but in contrast to CL, these do not mediate localized inflammatory responses.
Progress towards elimination of onchocerciasis in the Region du Sud-Ouest of Burkina Faso which was previously subject to a recrudescence event after vector control
Authors: Nikièma Achille Sindimbasba , Koala Lassane , Post Rory J. , Kima Appolinaire , Compaoré Justin , Kafando Claude M. , Nana Jean Baptiste , Bougouma Clarisse , Faye Babacar , Traoré Soungalo , Dabiré Roch Kounbobr
Published: April 2024

Background

The Sud-Ouest region of Burkina Faso (especially the Bougouriba valley) has been historically problematic with respect to onchocerciasis control, with a recrudescence of infections after vector control carried out the WHO Onchocerciasis Control Programme was halted in 1989. After 1996, mass drug administration of ivermectin was instigated to control the recrudescence so that it would no longer constitute a public health problem. However, in 2010 WHO changed its recommended policy from control to elimination, and in 2013 biannual Community-Directed Treatment with Ivermectin (CDTI) was instigated. Epidemiological surveys were carried-out in 2011 and 2018 to determine whether CDTI was producing a decline in infection levels and progress towards elimination.

Methodology/Principal findings

A cross-sectional study was conducted across 20 villages in four health districts in 2011 and 29 villages in 2018. Individuals aged five years and above were examined by skin-snip, and the prevalence and microfilarial load was determined for each village.

In 2011, 75% of villages had some infections and 20% had prevalences >5%, with a mean prevalence across all villages of 2.63% (range 0.0–9.7%), and community microfilarial load ranging from 0 to 0.25 microfilariae per biopsy. In 2018, nine villages (= 31% of total) had some infections, with prevalences ranging from 0.41% to 3.54%, and a mean prevalence across all villages of 0.37%. Community microfilarial load ranged from 0 to 0.1. Amongst those people found to be microfilarial positive, 87% had a history of migration.

Conclusions/significance

The endemicity of onchocerciasis infection in the Sud-Ouest region has declined to low levels and seems to be progressing towards elimination. Our findings indicated that biannual CDTI is having good effect, but it should continue for a number of years to ensure elimination of transmission. However, progress towards elimination has a troublesome history in this region, and it would be advisable to select more sentinel villages to have confidence in any future epidemiological and entomological surveys, especially Stop-MDA surveys. With positive individuals migrating between countries, cross-border collaboration needs more attention to ensure effective treatment for onchocerciasis elimination.
Utilization of technology to provide on-the-job trainings on Emergency Obstetric and Neonatal Care: Perspectives of nurses and midwives working in Rwanda’s remote health facilities
Authors: Uhawenimana Thierry Claudien , Gakwerere Mathias , Ngabonzima Anaclet , Yamuragiye Assumpta , Harindimana Florien , Ndayisenga Jean Pierre
Published: April 2024

Introduction

One of the targets for the third sustainable development goals is to reduce worldwide maternal mortality ratio (MMR) to less than 70 deaths per 100,000 live births by 2030. To address issues affecting women and the newborns during childbirth and postnatal period, concerted efforts from governments and their stakeholders are crucial to maximize the use of technology to enhance frontline health professionals’ skills to provide the emergency obstetric and newborn care (EmONC). However, no study has garnered nurses’ and midwives’ perspectives regarding the application of technology-enhanced learning approach to provide on-the-job Continuous Professional Development (CPD) and factors that may influence the application of this training approach in the Rwandan context.

Methods

The study collected data from nurses and midwives from forty (40) public health facilities in remote areas nationwide. The study applied a qualitative descriptive design to explore and describe nurses’ and midwives’ perspectives on the feasibility and acceptability of technology enhanced learning approaches such as e-learning, phone-based remote training, and other online methods to provide trainings in EmONC. Two focus group discussions with EmONC mentors, two with nurses and midwives were conducted. Twelve key informant interviews were conducted. Participants were selected purposively. In total, 54 individuals were included in this study. A thematic approach was used to analyse data.

Results

Nurses and midwives highlighted the need to provide refresher trainings about the management of pre-eclampsia. Most of the EmONC trainings are still provided face-to-face and the use of technology enhanced learning approaches have not yet been embraced in delivering EmONC CPDs for nurses and midwives in remote areas. Nurses and midwives found the first developed prototype of smartphone app training of the EmONC acceptable as it met the midwives’ expectations in terms of the knowledge and skills’ gap in EmONC.

Conclusion

Although the newly developed application was found acceptable, further research involving practical sessions by nurses and midwives using the developed application is needed to garner views about the ease of use of the application, relevance of the EmONC uploaded content on the app, and needed improvements on the app to address their needs in EmONC.
Will advancement in technologies bring fear and damage human employment? Evidence from China’s manufacturing industry
Authors: Wang Peng , Li Donghai , Wang Yangzi , Han Qingjiang , Khan Yousaf Ali
Published: April 2024

Advancement in technologies such as robotic industries and artificial intelligence bring fear among human being that jobs will be substituted by robots. Base on the panel data of 28 China’s manufacturing industries, this research analyzed the impact of technical progress bias on employment. First, we calculate the technical progress bias index of 28 industries base on the stochastic frontier model with transcendental logarithm function found 16 industries were toward the skilled labor while the remaining 12 industries were toward the unskilled labor. Second, the empirical results show that technical progress bias has a positive impact on the total manufacturing employment and significant positive effect on the unskilled labor, while no significant impact on skilled labor employment. Third, the threshold effect test proves that if taking industry value-added per capita or R&D capital stock as threshold variable, the threshold about the impact exist, making the impact on skilled labor was insignificant.
Influence of a change in activity regime on femoral bone architecture and failure behaviour
Authors: Villette Claire C. , Phillips Andrew T. M.
Published: April 2024

The incidence and morbidity of femoral fractures increases drastically with age. Femoral architecture and associated fracture risk are strongly influenced by loading during physical activities and it has been shown that the rate of loss of bone mineral density is significantly lower for active individuals than inactive. The objective of this work is to evaluate the impact of a cessation of some physical activities on elderly femoral structure and fracture behaviour. The authors previously established a biofidelic finite element model of the femur considered as a structure optimised to loading associated with daily activities. The same structural optimisation algorithm was used here to quantify the changes in bone architecture following cessation of stair climbing and sit-to-stand. Side fall fracture simulations were run on the adapted bone structures using a damage elasticity formulation. Total cortical and trabecular bone volume and failure load reduced in all cases of activity cessation. Bone loss distribution was strongly heterogeneous, with some locations even showing increased bone volume. This work suggests that maintaining the physical activities involved in the daily routine of a young healthy adult would help reduce the risk of femoral fracture in the elderly population by preventing bone loss.
Development of new materials for electrothermal metals using data driven and machine learning
Authors: Zhou Chengqun , Pei Muyang , Wu Chao , Xu Degang , Peng Qiang , He Guoai
Published: April 2024

After adopting a combined approach of data-driven methods and machine learning, the prediction of material performance and the optimization of composition design can significantly reduce the development time of materials at a lower cost. In this research, we employed four machine learning algorithms, including linear regression, ridge regression, support vector regression, and backpropagation neural networks, to develop predictive models for the electrical performance data of titanium alloys. Our focus was on two key objectives: resistivity and the temperature coefficient of resistance (TCR). Subsequently, leveraging the results of feature selection, we conducted an analysis to discern the impact of alloying elements on these two electrical properties.The prediction results indicate that for the resistivity data prediction task, the radial basis function kernel-based support vector machine model performs the best, with a correlation coefficient above 0.995 and a percentage error within 2%, demonstrating high predictive capability. For the TCR data prediction task, the best-performing model is a backpropagation neural network with two hidden layers, also with a correlation coefficient above 0.995 and a percentage error within 3%, demonstrating good generalization ability. The feature selection results using random forest and Xgboost indicate that Al and Zr have a significant positive effect on resistivity, while Al, Zr, and V have a significant negative effect on TCR. The conclusion of the composition optimization design suggests that to achieve both high resistivity and TCR, it is recommended to set the Al content in the range of 1.5% to 2% and the Zr content in the range of 2.5% to 3%.
Transsynaptic degeneration of ventral horn motor neurons exists but plays a minor role in lower motor system dysfunction in acute ischemic rats
Authors: Zhang Lei , Liu Jingwen , Liu Mingsheng
Published: April 2024

Background

As a leading cause of mortality and long-term disability, acute ischemic stroke can produce far-reaching pathophysiological consequences. Accumulating evidence has demonstrated abnormalities in the lower motor system following stroke, while the existence of Transsynaptic degeneration of contralateral spinal cord ventral horn (VH) neurons is still debated.

Methods

Using a rat model of acute ischemic stroke, we analyzed spinal cord VH neuron counts contralaterally and ipsilaterally after stroke with immunofluorescence staining. Furthermore, we estimated the overall lower motor unit abnormalities after stroke by simultaneously measuring the modified neurological severity score (mNSS), compound muscle action potential (CMAP) amplitude, repetitive nerve stimulation (RNS), spinal cord VH neuron counts, and the corresponding muscle fiber morphology. The activation status of microglia and extracellular signal-regulated kinase 1/2 (ERK 1/2) in the spinal cord VH was also assessed.

Results

At 7 days after stroke, the contralateral CMAP amplitudes declined to a nadir indicating lower motor function damage, and significant muscle disuse atrophy was observed on the same side; meanwhile, the VH neurons remained intact. At 14 days after focal stroke, lower motor function recovered with alleviated muscle disuse atrophy, while transsynaptic degeneration occurred on the contralateral side with elevated activation of ERK 1/2, along with the occurrence of neurogenic muscle atrophy. No apparent decrement of CMAP amplitude was observed with RNS during the whole experimental process.

Conclusions

This study offered an overview of changes in the lower motor system in experimental ischemic rats. We demonstrated that transsynaptic degeneration of contralateral VH neurons occurred when lower motor function significantly recovered, which indicated the minor role of transsynaptic degeneration in lower motor dysfunction during the acute and subacute phases of focal ischemic stroke.
Machine vision model for detection of foreign substances at the bottom of empty large volume parenteral
Authors: Yuan Pi , Li Chen , Tang Peng , Yuan Bin , Yin Yongjing
Published: April 2024

Empty large volume parenteral (LVP) bottle has irregular shape and narrow opening, and its detection accuracy of the foreign substances at the bottom is higher than that of ordinary packaging bottles. The current traditional detection method for the bottom of LVP bottles is to directly use manual visual inspection, which involves high labor intensity and is prone to visual fatigue and quality fluctuations, resulting in limited applicability for the detection of the bottom of LVP bottles. A geometric constraint-based detection model (GCBDM) has been proposed, which combines the imaging model and the shape characteristics of the bottle to construct a constraint model of the imaging parameters, according to the detection accuracy and the field of view. Then, the imaging model is designed and optimized for the detection. Further, the generalized GCBDM has been adopted to different bottle bottom detection scenarios, such as cough syrup and capsule medicine bottles by changing the target parameters of the model. The GCBDM, on the one hand, can avoid the information at the bottom being blocked by the narrow opening in the imaging optical path. On the other hand, by calculating the maximum position deviation between the center of visual inspection and the center of the bottom, it can provide the basis for the accuracy design of the transmission mechanism in the inspection, thus further ensuring the stability of the detection.
Mobility-oriented measurements of people’s exposure to outdoor artificial light at night (ALAN) and the uncertain geographic context problem (UGCoP)
Authors: Liu Yang , Kwan Mei-Po
Published: April 2024

Advanced nighttime light (NTL) remote sensing techniques enable the large-scope epidemiological investigations of people’s exposure to outdoor artificial light at night (ALAN) and its health effects. However, multiple uncertainties remain in the measurements of people’s exposure to outdoor ALAN, including the representations of outdoor ALAN, the contextual settings of exposure measurements, and measurement approaches. Non-exposed but included outdoor ALAN and causally irrelevant outdoor ALAN may manifest as contextual errors, and these uncertain contextual errors may lead to biased measurements and erroneous interpretations when modeling people’s health outcomes. In this study, we systematically investigated outdoor ALAN exposure measurements in different geographic contexts using either residence-based or mobility-oriented measurements, different spatial scales, and multiple NTL data sources. Based on the GPS data collected from 208 participants in Hong Kong, outdoor ALAN exposures were measured from NTL imagery at 10 m, 130 m, and 500 m spatial resolutions using in-situ methods or 100 m, 300 m, and 500 m buffer zone averaging. Descriptive analysis, multiple t-tests, and logistic regression were employed to examine the differences between outdoor ALAN exposure measurements using various contextual settings and their effects on modeling people’s overall health. Our results confirmed that different contextual settings may lead to significantly different outdoor ALAN exposure measurements. Our results also confirmed that contextual errors may lead to erroneous conclusions when using improper contextual settings to model people’s overall health. Consequentially, we suggest measuring people’s exposure to outdoor ALAN using the mobility-oriented approach, NTL representation with the high spatial resolution, and a very small buffer zone as a contextual unit to derive outdoor ALAN exposure. This study articulates essential methodological issues induced by uncertainties in outdoor ALAN exposure measurements and can provide essential implications and suggestions for a broad scope of studies that need accurate outdoor ALAN exposure measurements.
Is bovine somatotropin an alternative strategy to overcome the detrimental effects of high-gain diets on prepubertal Holstein × Gyr heifers?
Authors: Sguizzato Anna Luiza Lacerda , Guimarães Simone Eliza Facioni , Santos Giancarlo Magalhães , Santos Erollykens Ferreira , Marcondes Marcos Inácio
Published: April 2024

Feeding high-gain diets and an inadequate energy and protein ratio during pre-puberty may lead to impaired growth and mammary gland development of heifers. Thus, frequent application of bovine somatotropin (bST) may prevent future losses in productivity, improve mammary development and animal performance. We aimed to evaluate the effects of bST on digestibility, performance, blood metabolites, mammary gland development, and carcass composition of high-performance prepubertal Holstein × Gyr heifers. Thirty-four Holstein × Gyr heifers with an average initial body weight of 218 ± 49 kg and 14 ± 4 months of age were submitted to an 84-day trial evaluating the effects of no bST or bST injections. Treatments were randomly assigned to each animal within one of the tree blocks. The bST did not influence digestibility or performance parameters. Regarding blood results, IGF1 concentration presented an interaction between treatment and day, where bST heifers had the highest IGF1 concentration. Heifers receiving bST also showed increased ribeye area; however, only an experimental day effect for backfat thickness was observed, with greater accumulation of carcass fat on day 84. Heifers receiving bST had lower pixels/mm² on parenchyma, characteristic of greater parenchymal tissue. Moreover, heifers on bST treatment also had reduced pixels/mm2, characteristic of reduced fat pad tissue. Lastly, bST injections did not influence liver and muscle gene expression, nor most genes evaluated in mammary gland tissue, except for IGFBP3 expression, which was greater for bST heifers. In summary, we confirm the efficacy of bST injections to overcome the detrimental effects of high-gain diets on mammary gland growth and to improve lean carcass gain of prepubertal Holstein × Gyr heifers.
How policy promotes the integration of culture and tourism? A fuzzy-set qualitative comparative analysis based on the Policy Instrument Theory
Authors: Tang Yunyun , Yi Kaigang , He Zijian
Published: April 2024

Crafting pertinent policies to facilitate the high-level integration of culture and tourism has now become a vital agenda within the current discourse in China. However, relatively little is known about the actual implementation of various policies to achieve a high-level integration, especially how combinations of policy instruments are deployed in the process of realization. Based on the Policy Instrument Theory, this study uses fuzzy-set qualitative comparative analysis on a sample of 31 provincial administrative regions in China to investigate the influence of typical policy instruments on the integration level of tourism and culture. The results show that each single policy tool is not necessary for high-level integration of culture and tourism. On the contrary, only through an organic combination of different policy tools can affect the integration level. This study also summarizes five policy instrument configurations, which can be grouped into four driving modes of culture-tourism integration: the environment-driven supply-demand coordination mode, supply-driven demand-environment coordination mode, supply-driven mode, and supply-driven environment coordination mode. This study considerably provides critical theoretical and practical insights into the integration of culture and tourism from the perspective of governmental policies.
Real-time 3D tracking of swimming microbes using digital holographic microscopy and deep learning
Authors: Matthews Samuel A. , Coelho Carlos , Rodriguez Salas Erick E. , Brock Emma E. , Hodge Victoria J. , Walker James A. , Wilson Laurence G.
Published: April 2024

The three-dimensional swimming tracks of motile microorganisms can be used to identify their species, which holds promise for the rapid identification of bacterial pathogens. The tracks also provide detailed information on the cells’ responses to external stimuli such as chemical gradients and physical objects. Digital holographic microscopy (DHM) is a well-established, but computationally intensive method for obtaining three-dimensional cell tracks from video microscopy data. We demonstrate that a common neural network (NN) accelerates the analysis of holographic data by an order of magnitude, enabling its use on single-board computers and in real time. We establish a heuristic relationship between the distance of a cell from the focal plane and the size of the bounding box assigned to it by the NN, allowing us to rapidly localise cells in three dimensions as they swim. This technique opens the possibility of providing real-time feedback in experiments, for example by monitoring and adapting the supply of nutrients to a microbial bioreactor in response to changes in the swimming phenotype of microbes, or for rapid identification of bacterial pathogens in drinking water or clinical samples.
Quantifying Y chromosome loss in primary and metastatic prostate cancer by chromosome painting
Authors: Rajanala Sai Harisha , Ghale Romina , Nandakumar Subhiksha , Chadalavada Kalyani , Lee Gwo-Shu Mary , Stopsack Konrad H. , Chen Yu , Nanjangud Gouri J. , Chakraborty Goutam , Kantoff Philip W.
Published: April 2024

Somatic Y chromosome loss in hematopoietic cells is associated with higher mortality in men. However, the status of the Y chromosome in cancer tissue is not fully known due to technical limitations, such as difficulties in labelling and sequencing DNA from the Y chromosome. We have developed a system to quantify Y chromosome gain or loss in patient-derived prostate cancer organoids. Using our system, we observed Y chromosome loss in 4 of the 13 (31%) patient-derived metastatic castration-resistant prostate cancer (mCRPC) organoids; interestingly, loss of Yq (long arm of the Y chromosome) was seen in 38% of patient-derived organoids. Additionally, potential associations were observed between mCRPC and Y chromosome nullisomy. The prevalence of Y chromosome loss was similar in primary and metastatic tissue, suggesting that Y chromosome loss is an early event in prostate cancer evolution and may not a result of drug resistance or organoid derivation. This study reports quantification of Y chromosome loss and gain in primary and metastatic prostate cancer tissue and lays the groundwork for further studies investigating the clinical relevance of Y chromosome loss or gain in mCRPC.
Common dolphin whistle responses to experimental mid-frequency sonar
Authors: Casey Caroline , Fregosi Selene , Oswald Julie N. , Janik Vincent M. , Visser Fleur , Southall Brandon
Published: April 2024

Oceanic delphinids that occur in and around Navy operational areas are regularly exposed to intense military sonar broadcast within the frequency range of their hearing. However, empirically measuring the impact of sonar on the behavior of highly social, free-ranging dolphins is challenging. Additionally, baseline variability or the frequency of vocal state-switching among social oceanic dolphins during undisturbed conditions is lacking, making it difficult to attribute changes in vocal behavior to anthropogenic disturbance. Using a network of drifting acoustic buoys in controlled exposure experiments, we investigated the effects of mid-frequency (3–4 kHz) active sonar (MFAS) on whistle production in short-beaked (Delphinus delphis delphis) and long-beaked common dolphins (Delphinus delphis bairdii) in southern California. Given the complexity of acoustic behavior exhibited by these group-living animals, we conducted our response analysis over varying temporal windows (10 min– 5 s) to describe both longer-term and instantaneous changes in sound production. We found that common dolphins exhibited acute and pronounced changes in whistle rate in the 5 s following exposure to simulated Navy MFAS. This response was sustained throughout sequential MFAS exposures within experiments simulating operational conditions, suggesting that dolphins may not habituate to this disturbance. These results indicate that common dolphins exhibit brief yet clearly detectable acoustic responses to MFAS. They also highlight how variable temporal analysis windows–tuned to key aspects of baseline vocal behavior as well as experimental parameters related to MFAS exposure–enable the detection of behavioral responses. We suggest future work with oceanic delphinids explore baseline vocal rates a-priori and use information on the rate of change in vocal behavior to inform the analysis time window over which behavioral responses are measured.
Using full-text content to characterize and identify best seller books: A study of early 20th-century literature
Authors: da Silva Giovana D. , Silva Filipi N. , de Arruda Henrique F. , e Souza Bárbara C. , Costa Luciano da F. , Amancio Diego R.
Published: April 2024

Artistic pieces can be studied from several perspectives, one example being their reception among readers over time. In the present work, we approach this interesting topic from the standpoint of literary works, particularly assessing the task of predicting whether a book will become a best seller. Unlike previous approaches, we focused on the full content of books and considered visualization and classification tasks. We employed visualization for the preliminary exploration of the data structure and properties, involving SemAxis and linear discriminant analyses. To obtain quantitative and more objective results, we employed various classifiers. Such approaches were used along with a dataset containing (i) books published from 1895 to 1923 and consecrated as best sellers by the Publishers Weekly Bestseller Lists and (ii) literary works published in the same period but not being mentioned in that list. Our comparison of methods revealed that the best-achieved result—combining a bag-of-words representation with a logistic regression classifier—led to an average accuracy of 0.75 both for the leave-one-out and 10-fold cross-validations. Such an outcome enhances the difficulty in predicting the success of books with high accuracy, even using the full content of the texts. Nevertheless, our findings provide insights into the factors leading to the relative success of a literary work.
Bootstrap-quantile ridge estimator for linear regression with applications
Authors: Dar Irum Sajjad , Chand Sohail
Published: April 2024

Bootstrap is a simple, yet powerful method of estimation based on the concept of random sampling with replacement. The ridge regression using a biasing parameter has become a viable alternative to the ordinary least square regression model for the analysis of data where predictors are collinear. This paper develops a nonparametric bootstrap-quantile approach for the estimation of ridge parameter in the linear regression model. The proposed method is illustrated using some popular and widely used ridge estimators, but this idea can be extended to any ridge estimator. Monte Carlo simulations are carried out to compare the performance of the proposed estimators with their baseline counterparts. It is demonstrated empirically that MSE obtained from our suggested bootstrap-quantile approach are substantially smaller than their baseline estimators especially when collinearity is high. Application to real data sets reveals the suitability of the idea.
Mind the ramp: Association between early life ramp use and spatial cognition in laying hen pullets
Authors: Johny Alex , Janczak Andrew M. , Nordgreen Janicke , Toscano Michael J. , Stratmann Ariane
Published: April 2024

Ramps facilitate earlier access to complex environments and increase early life voluntary exercise, which may positively affect the cognitive development of chickens. This study focused on quantifying individual differences in ramp use and its impact on spatial cognition of laying hen pullets. Sixteen identical pens were housed with Lohmann Selected Leghorn (LSL) chicks of which eight chicks from each pen were colour marked from one day of age (DoA) to serve as focal birds. We quantified overall ramp use (walk/run, wing-assisted incline running, and jump/fly to and from ramps) by scan sampling recorded videos for 6, 10, 12, 20, 27, 41, and 55 DoA for all focal birds. From 56 to 95 DoA, long and short-term spatial memory of three focal birds per pen were assessed in a holeboard test in three consecutive phases: cued, uncued and reversal. Mixed model analysis showed that the spatial cognitive abilities of the birds were linked to differences in ramp use frequency averaged across all observation days. Birds with higher ramp use made fewer reference (Estimate ± Confidence Interval = 0.94 [0.88, 0.99], p = 0.08) and working memory errors (Est ± CI = 0.77 [0.59, 1.00], p = 0.06) in the cued phase than birds with lower ramp use. In contrast, birds with higher ramp use made more reference memory errors (Est ± CI = 1.10 [1.01, 1.20], p = 0.05) in the reversal phase. Birds with higher ramp use also made more reference memory errors compared to birds with lower ramp use as the phases changed from cued to uncued (p = 0.001). Our results indicate that there might be a relationship between early life ramp use and spatial cognition of laying hens.
Broken promises and transoceanic fragments: Japanese tango musicians in Manchuria, 1935–46
Authors: Asaba Yuiko
Published: April 2024

This article examines the personal experiences and memories of Japanese tango musicians in Manchuria in the years leading up to and immediately after the Second World War, revealing the tensions between migration and movement, on the one hand, and memory and loss, on the other. By engaging with the ideas surrounding tairiku (‘continents’) in early to mid-twentieth century Japan, this article moves away from triangulating the transoceanic movements of Japanese tango musicians and musical commodities across Japan, China and Latin America at this time. Instead, it considers such movements as the sonic manifestation of the island/continent dichotomy that framed Japanese maritime thinking in the first half of the twentieth century. Consequently, in offering a Japan-reflexive scholarship for the study and writing of global music histories, this article argues for the need to move beyond a geo-oceanic approach in examining transoceanic circulations.
‘When Will You Return’: The trans-border tangos of ‘Heri Jun Zailai’1
Authors: Nishimura Masao
Published: April 2024

This text discusses a well-known Chinese popular song, ‘When Will You Return’. The original recording of this song was performed with the accompaniment of western musicians. While the song has thereby been associated with both Chinese and tango imagery in Japan, it has also been subject to a variety of political interpretations. In China, meanwhile, it has been a site of frequently politicized criticism. In this article, I will attend broadly to its trans-border nature and then explore the mystery of the song’s composer and original accompanist.
Chinese Film Classics, 1922–1949, Christopher Rea (2021)
Authors: Lin Shu-mei
Published: April 2024

Review of: Chinese Film Classics, 1922–1949, Christopher Rea (2021)

New York: Columbia University Press, 381 pp.,

ISBN 978-0-23118-813-5, h/bk, $30.00/£25.00
The Power of Print in Modern China: Intellectuals and Industrial Publishing from the End of Empire to Maoist State Socialism, Robert Culp (2019)
Authors: Gao Selina J.
Published: April 2024

Review of: The Power of Print in Modern China: Intellectuals and Industrial Publishing from the End of Empire to Maoist State Socialism, Robert Culp (2019)

New York: Columbia University Press, 392 pp.,

ISBN 978-0-23118-416-8, h/bk, $65.00
The Making of Chinese-Sinophone Literatures as World Literature, Kuei-Fen Chiu and Yingjin Zhang (eds) (2022)
Authors: Ying Yan
Published: April 2024

Review of: The Making of Chinese-Sinophone Literatures as World Literature, Kuei-Fen Chiu and Yingjin Zhang (eds) (2022)

Hong Kong: Hong Kong University Press, 249 pp.,

ISBN 978-9-88852-872-1, h/bk, HKD 620.00
Transpacific Attachments: Sex Work, Media Networks, and Affective Histories of Chineseness, Lily Wong (2018)
Authors: Chen Po-hsi
Published: April 2024

Review of: Transpacific Attachments: Sex Work, Media Networks, and Affective Histories of Chineseness, Lily Wong (2018)

New York: Columbia University Press, 248 pp.,

ISBN 978-0-23118-338-3, h/bk, £55