bluuebunny/crossref_metadata_2025 huggingface dataset:
A within-person examination of the effect of mentors’ daily ostracism on protégés’ displaced aggression and in-role performance
Authors: Li Miaomiao , Wu Lunwen , Qin Yinglin
Published: February 2023

Purpose

Drawing on social information processing theory and social comparison theory, we test how mentors’ daily ostracism triggers protégés’ envy, thus leading to decreased in-role performance and increased displaced aggression.

Design/methodology/approach

Using an experience sampling study across three work weeks, the study provided theoretical and empirical examinations of dynamic, within-person processes related to mentors’ ostracism.

Findings

Mentors’ daily ostracism triggers protégés’ envy, which mediates the effect of mentors’ daily ostracism on protégés’ displaced aggression and in-role performance. Our findings supported our hypothesis of the buffering effect of mentorship quality on the negative effect of mentors’ ostracism on protégés’ envy but did not show a significant moderating effect on the mediating effect of protégés’ emotions between mentors’ daily ostracism and protégés’ behaviors.

Research limitations/implications

Our study focused on the victims of mentors’ ostracism on a daily basis. We constructed an overarching theoretical model to investigate how, why, and when mentors’ daily ostracism leads to protégés’ emotional and behavioral variability.

Practical implications

The study provided how to cope with ostracism and envy.

Originality/value

We discuss the theoretical implications of our findings for research on mentors’ ostracism, protégés’ emotions, and protégés’ behaviors.
The relationship between psychiatric nurses’ perceived organizational support and job burnout: Mediating role of psychological capital
Authors: Tang Yingxue , Wang Yingxuan , Zhou Haiying , Wang Juan , Zhang Rui , Lu Qinghua
Published: February 2023

Background

Psychiatric nurses need to keep close contact with patients suffering from mental illness. Because of the special nature of their profession, there is an increasing incidence of job burnout among psychiatric nurses.

Aim

This study examined the relationship between psychiatric nurses’ perceived organizational support, job burnout, and psychological capital. It also investigated the mediating role of psychological capital in the relationship between their perceived organizational support and job burnout.

Methods

A total of 916 psychiatric nurses were recruited from 6 grade-III mental facilities in Shandong Province using the stratified sampling approach. Their data were collected and examined using a general demographic data questionnaire, The Maslach Burnout Inventory, the Perceived Organizational Support Scale, and the Psychological Capital Questionnaire.

Results

The total score of job burnout was 53.71 ± 16.37. Specifically, 73.69% of the nurses had moderate to severe emotional exhaustion, 76.75% had moderate to severe job burnout pertaining to depersonalization, and 98.80% had moderate to severe job burnout pertaining to personal accomplishment. Spearman’s correlation analysis showed that both psychological capital (r = −0.35, p < 0.01) and perceived organizational support (r = −0.31, p < 0.01) were adversely related to job burnout. Additionally, psychological capital somewhat mediated the relationship between perceived organizational support and job burnout. Its mediating impact accounted for 33.20% of the overall effect.

Conclusion

This study’s participants had a moderate to severe level of job burnout. However, organizational support and psychological capital can be crucial in alleviating this problem among psychiatric nurses. Therefore, nursing managers and medical institutions should undertake timely and positive interventions to improve psychiatric nurses’ mental health and prevent job burnout. While exploring the impact of organizational support and psychological capital on job burnout, future studies should consider other effective influencing factors, and the relationship between the different factors should be explored in depth. This would provide a basis for developing a job burnout prevention mechanism.
Memory footprint: Predictors of flashbulb and event memories of the 2016 Euro Cup final
Authors: Ribeiro Andreia , Marques Margarida , Roberto Magda S. , Raposo Ana
Published: February 2023

Two years after Portugal won the UEFA European Championship, we examined what the Portuguese remember of this momentous occasion. We investigated if flashbulb memories (FBMs) and event memories (EMs) were determined by distinct factors, and whether EM was a predictor of FBM. Participants responded to an online questionnaire about their FBM, EM and set of predictors. Structural equation modeling revealed that FBM and EM were associated with different pathways. Interest in football predicted importance which triggered emotional intensity which predicted personal rehearsal, a direct determinant of FBMs. On the other pathway, interest determined knowledge about football, the main predictor of EMs. Importantly, EM was a causal determinant of FBM which shows that the memory trace for the original event enhances memory for the reception context. The findings suggests that even though the two types of memories are determined by independent factors, they interact very closely.
Passive and active suicidal ideation in a population-based sample of older adults: Associations with polygenic risk scores of relevance for suicidal behavior
Authors: Zettergren Anna , Jonson Mattias , Mellqvist Fässberg Madeleine , Najar Jenna , Rydberg Sterner Therese , Seidu Nazib M. , Kern Silke , Blennow Kaj , Zetterberg Henrik , Skoog Ingmar , Waern Margda
Published: February 2023

Introduction

There are few studies investigating genetic factors related to suicidal ideation or behavior in older adult populations. Our aim was to test associations between passive and active suicidal ideation and polygenic risk scores (PRSs) for suicidality and other traits of relevance for suicidality in old age (i.e. depression, neuroticism, loneliness, Alzheimer’s disease, cognitive performance, educational attainment, and several specified vascular diseases) in a population-based sample aged 70 years and older.

Methods

Participants in the prospective H70 study in Gothenburg, Sweden, took part in a psychiatric examination that included the Paykel questions on active and passive suicidal ideation. Genotyping was performed with the Neurochip (Illumina). After quality control of the genetic data the sample included 3467 participants. PRSs for suicidality and other related traits were calculated based on summary statistics from recent GWASs of relevance. Exclusion of persons with dementia or incomplete data on suicidal ideation yielded 3019 participants, age range 70–101 years. Associations between past year suicidal ideation (any level) and selected PRSs were analysed using general estimation equation (GEE) models, adjusted for sex and age.

Results

We observed associations between passive/active suicidal ideation and PRSs for depression (three versions), neuroticism, and general cognitive performance. After excluding individuals with current major depressive disorder (MDD), similar associations were seen with PRS for neuroticism, general cognitive performance and two PRSs for depression. No associations were found between suicidal ideation and PRSs for suicidality, loneliness, Alzheimer’s disease, educational attainment, or vascular disease.

Discussion

Our results could indicate which types of genetic susceptibility that are of importance for suicidality in old age, and these findings can help to shed light on potential mechanisms that may be involved in passive and active suicidal ideation in late-life, also in those with no current MDD. However, due to the limited sample size, the results need to be interpreted with caution until replicated in larger samples.
Application of the optimizing health literacy and access (Ophelia) process in partnership with a refugee community in Australia: Study protocol
Authors: Jawahar Zaman , Elmer Shandell , Hawkins Melanie , Osborne Richard H.
Published: February 2023

Refugees experience health inequities resulting from multiple barriers and difficulties in accessing and engaging with services. A health literacy development approach can be used to understand health literacy strengths, needs, and preferences to build equitable access to services and information. This protocol details an adaptation of the Ophelia (Optimizing Health Literacy and Access) process to ensure authentic engagement of all stakeholders to generate culturally appropriate, needed, wanted and implementable multisectoral solutions among a former refugee community in Melbourne, Australia. The Health Literacy Questionnaire (HLQ), widely applied around the world in different population groups, including refugees, is usually the quantitative needs assessment tool of the Ophelia process. This protocol outlines an approach tailored to the context, literacy, and health literacy needs of former refugees. This project will engage a refugee settlement agency and a former refugee community (Karen people origin from Myanmar also formerly knowns as Burma) in codesign from inception. A needs assessment will identify health literacy strengths, needs, and preferences, basic demographic data and service engagement of the Karen community. This community will be engaged and interviewed using a semi-structured interview based on the Conversational Health Literacy and Assessment Tool (CHAT) will cover supportive professional and personal relationships, health behaviors, access to health information, use of health services, and health promotion barriers and support. Using the needs assessment data, vignettes portraying typical individuals from this community will be developed. Stakeholders will be invited to participate in ideas generation and prioritization workshops for in-depth discussion on what works well and not well for the community. Contextually and culturally appropriate and meaningful action ideas will be co-designed to respond to identified health literacy strengths, needs, and preferences of the community. This protocol will develop and test new and improved methods that are likely to be useful for community-based organizations and health services to systematically understand and improve communication, services and outcomes among disadvantaged groups, particularly migrants and refugees.
Mapping frameworks and approaches to measuring the quality of transition support services for young people with intellectual and developmental disabilities
Authors: Beadle-Brown Julie , Šiška Jan , Káňová Šárka
Published: February 2023

Transition to adulthood for young people with intellectual disabilities and developmental disabilities (IDD) has been given significant attention in research, policy development and practice. The aim of this paper was to explore how a recently developed theoretical outcomes-based framework for measuring the quality of services for people with disabilities could potentially be useful in conceptualizing and supporting successful transition to adulthood. The theoretical discussion draws on both the scoping review and template analysis that was used to develop the Service Quality Framework and on a separate study synthesizing expert completed country templates and literature review which included models of and research on successful transition to adulthood. Synthesis identified that using a quality of life outcomes focused framework of Service Quality could be mapped onto and extend current thinking on what is seen as successful transition to adulthood by putting the focus on successful transition as people with IDD moving towards having similar opportunities and quality of life as other adults without disabilities living in the same community/society. Implications of a more wide-ranging definition and holistic view for both practice and future research are discussed.
Case Report: Wernicke's encephalopathy after gastric surgery presenting as lactic acidosis and refractory thrombocytopenia
Authors: Lin Qi , Li Guanghua , Wang Zhixiong , Zhang Yu
Published: February 2023

Wernicke's encephalopathy (WE) is a severe neuropsychiatric disorder, mainly resulting from a nutritional deficiency of thiamine. WE is hard to detect at an early stage. Less than 20% of WE can be diagnosed during a patient’s lifetime, and WE tends to occur in patients with chronic alcoholism. Therefore, a large proportion of non-alcoholic WE patients are misdiagnosed. Lactate is an important by-product of anaerobic metabolism when the aerobic metabolism is blocked without thiamine, which can potentially serve as an alerting index for WE. Here, we report a case of a patient with WE who suffered gastric outlet obstruction following postoperative fasting, accompanied by lactic acidosis and refractory thrombocytopenia. A 67-year-old non-alcoholic woman who suffered hyperemesis for 2 months was diagnosed with gastric outlet obstruction (GOO). Gastric biopsies with endoscopy revealed gastric cancer, and total gastrectomy, together with D2 nodal dissection, was performed. She developed a coma with refractory thrombocytopenia rapidly after the surgical procedures were performed. The above conditions were treated not by the administration of antibiotics but by that of thiamine. We also found before the start of the procedures that she had a high level of blood lactate for a long period of time. Early diagnosis of WE is important because permanent injury can be caused to the central nervous system. Even today, the diagnosis of WE mainly depends on clinical symptoms, but occasionally, a typical triad occurs among WE patients. Therefore, a sensitive index for early diagnosis is critical for WE. Rising levels of blood lactate as a result of thiamine deficiency can serve as a warning for WE. In addition, we noted that this patient had a non-typical thiamine-sensitive refractory thrombocytopenia.
Case report: Hilar metastasis of breast cancer: A single-center retrospective case-control study
Authors: Yang Ruohan , Jia Lin , Lv Zheng , Cui Jiuwei
Published: February 2023

Purpose

The lungs are a common metastatic organ in breast cancer, mainly due to blood metastasis. On imaging, most metastatic lesions show a peripheral round mass in the lung, occasionally with a hilar mass as the primary manifestation, showing burr and lobulation signs. This study aimed to investigate breast cancer patient's clinical characteristics and prognosis with two different metastatic sites in the lung.

Methods

We retrospectively analyzed patients admitted to the First Hospital of Jilin University between 2016 and 2021 diagnosed with breast cancer lung metastases. Forty breast cancer patients with hilar metastases (HM) and 40 patients with peripheral lung metastases (PLM) were matched 1:1 using a pairing method. To analyze the patient's prognosis, the clinical characteristics of patients with two different metastatic sites were compared using the chi-square test, Kaplan–Meier curve, and Cox proportional hazards model.

Results

The median follow-up time was 38 months (2–91 months). The median age of patients with HM was 56 years (25–75 years), and that of patients with PLM was 59 years (44–82 years). The median overall survival (mOS) was 27 months in the HM group and 42 months in the PLM group (p = 0.001). The results of the Cox proportional hazards model showed that the histological grade (hazard ratio = 2.741, 95% confidence interval 1.442–5.208, p = 0.002) was a prognostic factor in the HM group.

Conclusion

The number of young patients in the HM group was higher than that in the PLM group, with higher Ki-67 indexes and histological grades. Most patients had mediastinal lymph node metastasis, with shorter DFI and OS and poor prognosis.
Failure to rescue following anatomical lung resection. Analysis of a prospective nationwide database
Authors: Gómez-Hernández María Teresa , Rivas Cristina , Novoa Nuria , Jiménez Marcelo F. ,   the Spanish Group of Video-assisted Thoracic Surgery (GEVATS)
Published: February 2023

Background

Rescue failure has been described as an important factor that conditions postoperative mortality after surgical interventions. The objective of this study is to determine the incidence and main determinants of failure to rescue after anatomical lung resections.

Methods

Prospective multicenter study that included all patients undergoing anatomical pulmonary resection between December 2016 and March 2018 and registered in the Spanish nationwide database GEVATS. Postoperative complications were classified as minor (grades I and II) and major (grades IIIa to V) according to the Clavien-Dindo standardized classification. Patients that died after a major complication were considered rescue failure. A stepwise logistic regression model was created to identify predictors of failure to rescue.

Results

3,533 patients were analyzed. In total, 361 cases (10.2%) had major complications, of which 59 (16.3%) could not be rescued. The variables associated with rescue failure were: ppoDLCO% (OR, 0.98; 95% CI, 0.96–1; p = 0.067), cardiac comorbidity (OR, 2.1; 95% CI, 1.1–4; p = 0.024), extended resection (OR, 2.26; 95% CI, 0.94–5.41; p = 0.067), pneumonectomy (OR, 2.53; 95 CI, 1.07–6.03; p = 0.036) and hospital volume <120 cases per year (OR, 2.53; CI 95%, 1.26–5.07; p = 0.009). The area under the curve of the ROC curve was 0.72 (95% CI: 0.64–0.79).

Conclusion

A significant percentage of patients who presented major complications after anatomical lung resection did not survive to discharge. Pneumonectomy and annual surgical volume are the risk factors most closely related to rescue failure. Complex thoracic surgical pathology should be concentrated in high-volume centers to obtain the best results in potentially high-risk patients.
Mental Health Screening for Korean Ukrainian Refugee Minors in the Republic of Korea: A Cross Sectional Pilot Study
Authors: Park Sejeong , Lee Jenny Seongryung , Kim Hye-Jung , Lee Hojung , Lee Myungjoo , Kim Soo-Yeon , Choi Han
Published: February 2023

Since February 2022, the Ukrainian refugee crisis has been highlighting mental health problems associated with trauma and distress. This study aimed to evaluate the mental health status of twenty-seven refugee minors (10 to 18 years old) who fled Ukraine and temporarily settled in the Republic of Korea (ROK). This cross-sectional survey study aimed to evaluate the mental health status of ethnic Korean Ukrainian refugee minors. The Child and Adolescent Trauma Screen—Youth Report (CATS), generalized anxiety disorder seven-item scale (GAD-7), and subjective unit of distress scale (SUDs) were used for assessment. A preliminary analysis indicated that 77% Ukrainian refugee minors were exposed to and experienced war-related trauma. They are at a relatively low risk of trauma symptoms, anxiety, and distress due to stable family and visa status and a comparably better environment in the ROK. Meanwhile, refugee teenagers showed higher rates of psychological distress compared with refugee children. This finding suggests that an early psychological interventions in a host country may be beneficial to prevent mental health issues in refugee minors.
Aerodynamic Modeling and Simulation of Multi-Lifting Surfaces Based on the Unsteady Vortex Lattice Method
Authors: Gao Wei , Liu Yishu , Li Qifu , Lu Bei
Published: February 2023

Using the unsteady vortex lattice method based on the potential flow theory, a rapid modeling approach is developed for the aerodynamic computation of multi-lifting surfaces. Multiple lifting surfaces with different geometric parameters and grid divisions can be quickly integrated and meshed with the object-oriented data structure. The physical influence between different lifting surfaces was modeled, and the wake–surface interaction was also considered by using different built-in vortex core models. The trajectory data were used to replace the pre-calculated downwash superposition for boundary condition integration, and the instantaneous boundary condition was generated directly from the kinematic states and mesh messages of the model concerned. Considering the direct coupling effect between aerodynamics and rigid body dynamics, the function for free flight was built for medium-fidelity dynamic simulations and aerodynamic data identifications. The proposed high-efficiency modeling and simulation process can be easily applied to models with any number of different lifting surfaces and arbitrary motion modes.
Regulation of bta-miRNA29d-3p on Lipid Accumulation via GPAM in Bovine Mammary Epithelial Cells
Authors: Zhao Xin , Li Jun , Zhao Shuying , Chen Lili , Zhang Man , Ma Yi , Yao Dawei
Published: February 2023

MicroRNAs (miRNAs) are small RNA molecules consisting of approximately 22 nucleotides that are engaged in the regulation of various bio-processes. There is growing evidence that miR-29 is a key regulator of hepatic lipid metabolism. Mimics and inhibitors of bta-miRNA29d-3p were transiently transfected in bovine mammary epithelial cells (BMECs) to reveal the regulation of bta-miRNA29d-3p on lipid accumulation in BMECs. Results showed that overexpression of bta-miRNA29d-3p significantly inhibited the expression of genes related to triglyceride (TAG) synthesis, namely DGAT1 and mitochondrial glycerol-3-phosphate acyltransferase (GPAM, p < 0.01) and down-regulated TAG levels in cells (p < 0.05). The expression of fatty acid synthesis and desaturation-related genes FASN, SCD1, and ACACA, and transcription factor SREBF1 also decreased. Interference of bta-miRNA29d-3p significantly increased the expression of GPAM, DGAT1, FASN, SCD1, ACACA, and SREBF1 (p < 0.01), and significantly upregulated the concentration of TAG in cells. Furthermore, a luciferase reporter assay confirmed that GPAM is a direct target of bta-miRNA29d-3p. In summary, bta-miRNA29d-3p modulates fatty acid metabolism and TAG synthesis by regulating genes related to lipid metabolism in BMECs and targeting GPAM. Thus, bta-miRNA29d-3p plays an important role in controlling mammary lipid synthesis in cows.
An Application of Partial Least Squares Structural Equation Modeling (PLS-SEM) to Examining Farmers’ Behavioral Attitude and Intention towards Conservation Agriculture in Bangladesh
Authors: Tama Riffat Ara Zannat , Hoque Md Mahmudul , Liu Ying , Alam Mohammad Jahangir , Yu Mark
Published: February 2023

Despite being widely advocated as a climate-smart farming system, the adoption of conservation agriculture (CA) among Bangladeshi farmers has remained surprisingly low. Evidence indicates that farmers’ behavior regarding the adoption and continuation of CA is affected by their socioeconomic and psychological factors. This study combined the Technology Acceptance Model (TAM) and Diffusion of Innovation (DOI) theories to examine the socio-psychological determinants of Bangladeshi farmers’ behavior regarding the adoption of CA. The proposed model included both reflective and formative measurements. Based on data collected from 201 CA farmers, this research used a variance-based structural equation modeling (PLS-SEM) approach to test the model. The analysis showed that the components of this integrated model explained more variance (Intention: 48.9%; Attitude: 59.2%) than the original TAM framework (Intention: 45.8%; Attitude: 54.5%). Farmers’ attitudes toward the continuation of CA were most influenced by the Relative Advantage (RA) of CA (β = 0.337). The low level of Complexity (β = 0.225) and Compatibility (β = 0.273) of CA had a significant positive effect on attitude. In a campaign to encourage farmers to act more sustainably, interventions should emphasize CA’s long-term benefits, such as its effects on soil, yield, and the environment.
Leveraging on Advanced Remote Sensing- and Artificial Intelligence-Based Technologies to Manage Palm Oil Plantation for Current Global Scenario: A Review
Authors: Akhtar Mohammad Nishat , Ansari Emaad , Alhady Syed Sahal Nazli , Abu Bakar Elmi
Published: February 2023

Advanced remote sensing technologies have undoubtedly revolutionized palm oil industry management by bringing business and environmental benefits on a single platform. It is evident from the ongoing trend that remote sensing using satellite and aerial data is able to provide precise and quick information for huge palm oil plantation areas using high-resolution image processing, which is also recognized by the certification agencies, i.e., the Roundtable on Sustainable Palm Oil (RSPO) and ISCC (International Sustainability and Carbon Certification). A substantial improvement in the palm oil industry could be attained by utilizing the latest Geo-information tools and technologies equipped with AI (Artificial Intelligence) algorithms and image processing, which could help to identify illegal deforestation, tree count, tree height, and the early detection of diseased leaves. This paper reviews some of the latest technologies equipped with remote sensing, AI, and image processing for managing the palm oil plantation. This manuscript also highlights how the distress in the current palm oil industry could be handled by mentioning some of the improvised monitoring systems for palm oil plantation that could in turn increase the yield of palm oil. It is evident from the proposed review that the accuracy of AI algorithms for palm oil detection depends on various factors such as the quality of the training data, the design of the neural network, and the type of detection task. In general, AI models have achieved high accuracy in detecting palm oil tree images, with some studies reporting accuracy levels up to 91%. However, it is important to note that accuracy can still be affected by factors such as variations in lighting conditions and image resolution. Nonetheless, with any AI model, the accuracy of algorithms for palm oil tree detection can be improved by collecting more diverse training data and fine-tuning the model.
Land Use and Land Cover Change Determinants in Raya Valley, Tigray, Northern Ethiopian Highlands
Authors: Gidey Eskinder , Dikinya Oagile , Sebego Reuben , Segosebe Eagilwe , Zenebe Amanuel , Mussa Said , Mhangara Paidamwoyo , Birhane Emiru
Published: February 2023

Land use and land cover change (LULCC) is the result of both natural and socio-economic determinants. The aim of this study was to model the determinant factors of land cover changes in Raya Valley, Southern Tigray, Ethiopia. Multistage sampling was used to collect data from 246 households sampled from lowlands (47), midlands (104), highlands (93), and sub-alpine (2) agro-climatological zone. Descriptive statistics and logit regression model were used to analyze the field survey data. Agricultural land expansion, fuelwood extraction, deforestation, overgrazing and expansion of infrastructure were the proximate causes of LULCC in the study area. Agricultural land expansion (p = 0.084) and wood extraction for fuel and charcoal production (p = 0.01) were the prominent causes for LULCC. Persistent drought (p = 0.001), rapid population growth (p = 0.027), and climate variability (p = 0.013) were the underlying driving factors of LULCC. The determinants of LULCC need to be considered and mitigated to draw robust land use policy for sustainable land management by the smallholder farmers. This study provides important results for designing and implementing scientific land management strategies by policy makers and land managers.
The Tradeoff between Maintaining Maize (Zea mays L.) Productivity and Improving Soil Quality under Conservation Tillage Practice in Semi-Arid Region of Northeast China
Authors: Chen Nana , Zhao Xin , Dou Shuxian , Deng Aixing , Zheng Chengyan , Cao Tiehua , Song Zhenwei , Zhang Weijian
Published: February 2023

Conservation tillage has received strong support globally to achieve food security and minimize environmental impacts. However, there are comprehensive debates on whether it can achieve the synergy between maintaining crop yields and improving soil quality. To this end, a field experiment under continuous maize (Zea mays L.) cropping was conducted in northeast China. The treatment included rotary tillage with straw removal (CK, conventional tillage) and rotary tillage, subsoiling tillage, and no tillage with straw retention (CR, CS, and CN, respectively). Maize yield and a set of soil physio-chemical indicators in relation with soil quality were measured during 2017 to 2021. Results showed that CN significantly reduced the maize yield by 24.9%, 23.1%, and 19.5% on average compared to that with CR, CK, and CS treatments, respectively. CN and CS significantly increased the ratio of >2 mm soil aggregates and soil geometric mean diameter (GMD) in the 0–20 cm soil layer compared those of CK and CR treatments. However, CN and CS treatments had a higher soil bulk density and soil compaction in the 0–20 cm layer compared to those with CK and CR treatments. Soil organic carbon and total nitrogen in the 0–20 cm layer under CN and CS were higher than those with CK by 5.1–15.0% and 8.5–15.7%, whereas soil NH4+ was lower by 9.1–13.9% correspondingly. CN also reduced the soil temperature during the early-growth stage of maize. Importance analysis indicated that soil temperature, bulk density, and available nitrogen were the key factors affecting maize yield. Overall, no tillage with straw mulching could improve soil stability and soil fertility but reduced maize yield. Alternatively, minimum tillage (e.g., subsoiling tillage) with straw mulching might be a suitable practice as it maintains the maize yield and improves soil quality compared to those with conventional tillage practices in the semi-arid region of northeast China in the short term.
Integrated Metabolome and Transcriptome Analysis Unveils the Underlying Molecular Response of Panax ginseng Plants to the Phytophthora cactorum Infection
Authors: Kan Hong , Qu Shuai , Dong Kai , Wang Shihan , Xu Chen , Wang Yingping , Hua Shuang
Published: February 2023

Due to at least 3 years of cultivation, Panax ginseng (ginseng) is susceptible to being attacked by pathogens which severely affect its quality and yield. Compared with other diseases of ginseng, Phytophthora blight caused by Phytophthora cactorum (P. cactorum) can spread rapidly and destroy almost the entire plant of ginseng, such as leaves, stems, and roots. However, little research was focused on this area, and how P. cactorum affected the metabolic profile of ginseng is still obscure. In the current study, we conducted a comprehensive analysis of metabolomics and transcriptomics to compare the differences in health and P. cactorum-affected ginseng leaves and stems. Metabolome analysis revealed that 110 and 113 significant differential metabolites were observably disturbed separately in ginseng leaves and stems. Transcriptome analysis demonstrated that 6424 and 9508 genes had remarkable variation in ginseng leaves and stems. Using conjoint analysis, we also revealed the changes in pathways “Alanine, aspartate and glutamate metabolism”, “Glycine, serine and threonine metabolism”, and “Biosynthesis of unsaturated fatty acids” and “Plant hormone signal transduction” in ginseng response to the P. cactorum. The current work provides an overview of the alteration of metabolic profile and gene expression profiles in ginseng leaves and stems in response to P. cactorum affection, which may help to further screen out the mechanism of plant-pathogen interaction at the molecular level.
Unravelling the Recent Developments in the Production Technology and Efficient Applications of Biochar for Agro-Ecosystems
Authors: Kumari Khushbu , Kumar Raushan , Bordoloi Nirmali , Minkina Tatiana , Keswani Chetan , Bauddh Kuldeep
Published: February 2023

Considerable interest is being shown in using biochar production from waste biomass with a variety of disciplines to address the most pressing environmental challenges. Biochar produced by the thermal decomposition of biomass under oxygen-limited conditions is gaining popularity as a low-cost amendment for agro-ecosystems. The efficiency of biochar formation is affected by temperature, heating rate, feedstock type, particle size and reactor conditions. Properties such as pH, surface area and ash content of produced biochar increases with increasing temperatures. Biochar produced at lower heating rates may have high porosity and be beneficial for morphological changes in the soil. Biochar can help to enhance soil health and fertility as well as improve agricultural yield. As a result, biochar can assist in increasing food security by promoting sustainable agricultural systems and preserving an eco-friendly environment. Biochar is also widely being used as a sorbent for organic and inorganic pollutants, owing to its large surface area, allowing it to be immobilized from soil with ease. The functional groups and charges present on the surface of biochar play an important role in pollutants removal. This review focuses on the mechanisms of biochar production using different waste materials as a feed stock, factors that influence biochar quality as well as application of biochar in agricultural soil and their reclamation as well. This article also discusses knowledge gaps and future perspectives in the field of biochar-based toxic-pollution remediation.
A Pleiotropic Drug Resistance Transporter TaABCG36 Contributes to Defense against Puccinia triticina in Triticum aestivum
Authors: Zhang Na , Hu Yaya , Wu Yanhui , Mapuranga Johannes , Yuan Ying , Yang Wenxiang
Published: February 2023

ABC transporters play important roles in plant growth and resistance to abiotic and biotic stresses. Here, we showed that the TaABCG36 gene positively regulates leaf rust resistance in the wheat line Thatcher + Lr19 (TcLr19) when challenged with an avirulent pathotype of Puccinia triticina (Pt). The TaABCG36 gene was cloned from genomic DNA and cDNA from wheat line TcLr19. The clone was 6730 bp in gDNA and 4365 bp in cDNA for this gene. It encoded an ABC transporter with 1454 amino acids in length. BLASTp analysis indicated a considerable identity ABC transporter G family member 36 with Aegilops tauschii subsp. strangulata, Triticum dicoccoides, and T. aestivum; thus, we named the gene TaABCG36. TaABCG36 was proved to be a plasma transmembrane protein by bioinformatic analysis and subcellular localization of the TaABCG36–GFP fusion protein. The expression of TaABCG36 in wheat leaves reached a peak at 72 h post-inoculation by Pt avirulence pathotype, and the expression was also induced by phytohormone treatments of salicylic acid (SA), abscisic acid (ABA), and methyl jasmonate (MeJA). Three fragments (V1–V3) of the TaABCG36 gene were introduced to the BSMV-VIGS vector and, thus, silenced the expression of TaABCG36 in the wheat line TcLr19. All the three BSMV:VIGS-infected plants showed reaction type “3” to Pt pathotype THTS, which was fully avirulent on TcLr19 (infection type “0”). Histopathological observation showed that silencing of TaABCG36 facilitated the formation of haustorial mother cells (HMC) and mycelial growth, implying that TaABCG36 plays a positive role in the response of TcLr19 against THTS. These results provide molecular insight into the interaction between Pt and its wheat host and identify a potential target for engineering resistance in wheat to damaging pathogen of Pt.
Grassland Ecosystem Progress: A Review and Bibliometric Analysis Based on Research Publication over the Last Three Decades
Authors: Zhu Xiaoyu , Zheng Jianhua , An Yi , Xin Xiaoping , Xu Dawei , Yan Ruirui , Xu Lijun , Shen Beibei , Hou Lulu
Published: February 2023

Understanding the grassland ecosystem is crucial for improving grassland ecosystem functions and services such as climate regulation, water and soil conservation, carbon sequestration, and biodiversity and gene pool maintenance. However, a systematic and comprehensive review of the relevant literature is still unclear and lacking. The VOSviewer software and cluster analysis were used to visually analyze and perform dimension reduction classification on the 27,778 studies related to grassland ecosystem research based on the Web of Science database. The number of publications targeting grassland ecosystem increased rapidly from 2006 to 2021. Ecology, agronomy, and environmental science were the most popular research categories, and the top journal sources were Remote Sensing, Journal of Ecology, and Ecology and Evolution. The leading publishing countries were the United States, China, and Germany. The top three institutions were the Chinese Academy of Sciences, the University of Chinese Academy of Sciences, and Colorado State University. Cooperation between different countries and institutions had increased. Keyword co-occurrence network analysis showed that Biodiversity, Vegetation and Conservation were the most popular study areas, grassland management, climate change, land use pattern, and ecosystem impact were the hot research topics. All studies could be divided into three categories by cluster analysis: grassland ecological characteristics including basic physicochemical properties, vegetation community characteristics, aboveground and belowground biomass, and soil structural quality of grassland; driving mechanisms that demonstrated effects of human activities and climate change on grassland ecosystem function; and grassland ecosystem services that focused the influences of different grassland management strategies on ecological services, animal welfare and human well-being. The three topic categories of reviewed studies were interrelated and consistent with each other, and the performances were progressive. This paper reviewed the trend evolution through keyword hotspots and analyzed the future research directions to provide an important reference for scientists to better respond to the balance of herbage and sustainable utilization of grassland and maintenance of ecological security.
The Activity of Calcium Glycerophosphate and Fluoride against Cariogenic Biofilms of Streptococcus mutans and Candida albicans Formed In Vitro
Authors: Cavazana Thamires Priscila , Hosida Thayse Yumi , Sampaio Caio , de Morais Leonardo Antônio , Monteiro Douglas Roberto , Pessan Juliano Pelim , Delbem Alberto Carlos Botazzo
Published: February 2023

This study evaluated the effects of calcium glycerophosphate (CaGP), with or without fluoride (F), on dual-species biofilms of Streptococcus mutans and Candida albicans. The biofilms were treated three times with 0.125, 0.25, and 0.5% CaGP solutions, with or without 500 ppm F (NaF). Additionally, 500 and 1100 ppm F-solutions and artificial saliva served as controls. After the final treatment, the microbial viability and biofilm structure, metabolic activity, total biomass production, and the composition of the extracellular matrix composition were analyzed. Regardless of the presence of F, 0.25 and 0.5% CaGP promoted a higher biomass production and metabolic activity increase than the controls (p < 0.05). F-free CaGP solutions reduced bacterial cell population significantly more than the 500 ppm F group or the negative control (p < 0.05). All the groups reduced the proteins, and 0.5% CaGP combined with F led to the highest reduction in the carbohydrate and nucleic acids content of the extracellular matrix (p < 0.05). It can be concluded that CaGP alone affected the number of bacterial cells and, when combined with F, reduced its production of biomass, metabolic activity, and the expression of the extracellular matrix components.
Low-Level Tetracycline Resistance Gene tet(O)_3 in Campylobacter jejuni
Authors: Pacífico Cátia , Wösten Marc M. S. M. , Hilbert Friederike
Published: February 2023

Campylobacter (C.) spp. are the most important foodborne, bacterial, and zoonotic pathogens worldwide. Resistance monitoring of foodborne bacterial pathogens is an important tool to control antimicrobial resistance as a part of the “One Health” approach. The detection and functionality of new resistance genes are of paramount importance in applying more effective screening methods based on whole genome sequencing (WGS). Most tetracycline-resistant C. spp. isolates harbor tet(O), a gene that encodes a ribosomal protection protein. Here we describe tet(O)_3, which has been identified in two food isolates of C. jejuni and is very similar to the tet(O) gene in Streptococcus pneumoniae, having a truncated promoter sequence. This gene confers resistance to tetracycline below 1 mg/L, which is the epidemiological cut-off value. We have analyzed the entire genome of these two isolates, together with a C. jejuni isolate found to have high-level resistance to tetracycline. In contrast to the highly resistant isolate, the promoter of tet(O)_3 is highly responsive to tetracycline, as observed by reverse transcription polymerase chain reaction (RT-PCR). In addition, the two isolates possess a CRISPR repeat, fluoroquinolone resistance due to the gyrA point mutation C257T, a β-lactamase resistance gene blaOXA-184, a multidrug efflux pump CmeABC and its repressor CmeR, but no plasmid. Low-level antibiotic resistant C. jejuni might therefore have an advantage for surviving in non-host environments.
Prospecting Plant Extracts and Bioactive Molecules with Antimicrobial Activity in Brazilian Biomes: A Review
Authors: de Queiroz José Carlos Eloi , Leite José Roberto S. A. , Vasconcelos Andreanne Gomes
Published: February 2023

Antimicrobial resistance is currently one of the greatest threats to global health, food security, and development. In this aspect, medicinal plants have been studied to support the development of viable alternatives to prevent and treat infectious diseases. This study aimed to perform a review of the literature comprising the antimicrobial activity of vegetable species from Brazilian biomes. We selected 67 original scientific publications about extracts, fractions, or isolated molecules from plants in the Brazilian biomes, published between 2016 and 2020 in Pubmed, ScienceDirect, and Scielo. Data demonstrated that 98 plant species, especially collected in the Cerrado, Atlantic Forest, and Caatinga biomes, were tested against 40 fungi and 78 bacterial strains. Bioactive fractions of Eucalyptus globulus methanolic stump wood extract were active against Candida albicans and C. tropicalis (MIC 2.50 µg/mL). The catechin purified from Banisteriopsis argyrophylla leaves had activity against C. glabrata (MIC 2.83 µg/mL) and ethanolic extract obtained from Caryocar coriaceum bark and fruit pulp exhibited MIC of 4.1 µg/mL on Microsporum canis. For bacteria, compounds isolated from the dichloromethane extract of Peritassa campestris, lectin extracted from a saline extract of Portulaca elatior and essential oils of Myrciaria pilosa exhibited significant effect against Bacillus megaterium (MIC 0.78 µg/mL), Pseudomonas aeruginosa (MIC 4.06 µg/mL) and Staphylococcus aureus strains (MIC 5.0 µg/mL), respectively. The findings support the antimicrobial and bioeconomic potential of plants from Brazilian biodiversity and their promising health applications.
Antioxidant Potential of Tamarillo Fruits—Chemical and Infrared Spectroscopy Analysis
Authors: Rito Miguel , Marques Joana , da Costa Ricardo M. F. , Correia Sandra , Lopes Tércia , Martin Daniel , Canhoto Jorge M. P. L. , Batista de Carvalho Luís A. E. , Marques Maria Paula M.
Published: February 2023

Native to South America, tamarillo (Solanum betaceum Cav.) is a small tree cultivated as a fruit crop in several regions of the world. Known for its sweet and sour taste, tamarillo fruits are very nutritious due to the presence of health-beneficial components such as fiber, vitamins, and antioxidants. Despite its nutritional value, tamarillo remains poorly known in global markets. The present work aims to study the antioxidant activity of four genotypes of tamarillo. Several chemical assays were performed to assess the antioxidant components and antioxidant activity of aqueous ethanolic extracts from each genotype. Overall, the Mealhada genotype (a red cultivar) showed the most interesting results, displaying the highest amount of total phenolic, flavonoids, and anthocyanin contents, as well as higher antioxidant activity. To evaluate the composition of the extract, Fourier-transform infrared spectroscopy (FTIR) was used to characterize important components in aqueous ethanolic extracts of the fruits, having revealed the presence of high amounts of phenols (the main compounds responsible for antioxidant activity), as well as triterpenoids and polysaccharides. The present results highlight the potential nutraceutical importance of tamarillo fruits.
An Explorative Study into the Aetiology of Developmental Dysplasia of the Hip Using Targeted Urine Metabolomics
Authors: Rhodes Amanda M. L. , Ali Sehrish , Minnion Magdalena , Lee Ling H. , Joseph Brijil M. , Ndzo Judwin , Clarke Nicholas M. P. , Feelisch Martin , Aarvold Alexander
Published: February 2023

Developmental dysplasia of the hip (DDH) is the most prevalent congenital musculoskeletal disorder, yet its cause remains unknown. Adequate nutrient provision and coordinated electron exchange (redox) processes are critical for foetal growth and tissue development. This novel study sought to explore specific biochemical pathways in skeletal development for potential involvement in the aetiology of DDH. Spot urine samples were collected from infants, aged 13–61 days, with and without DDH. Ion chromatography-mass spectrometry was used to quantify thiosulphate, sulphate, nitrate, and phosphate, whilst nitrite was quantified using high-performance liquid chromato-graphy. Thiobarbituric acid reactive substances (TBARS) were measured as markers of lipid peroxidation. Creatinine and osmolality were determined by a 96-well plate assay and micro-osmometer to potentially normalise values for renal function, lean body mass, and hydration status. Urine samples were analysed from 99 babies: 30 with DDH and 69 age-matched non-DDH controls. Thiosulphate, TBARS, and creatinine concentrations differed between the DDH group and the controls (p = 0.025, 0.015, and 0.004 respectively). Urine osmolality was significantly lower in DDH compared to the controls (p = 0.036), indicative of the production of a more diluted urine in DDH infants. Following adjustment for osmolality, significant differences became apparent in urinary sulphate levels in DDH (p = 0.035) whereas all other parameters were similar between the groups. This is the first study to assess the potential role of these inorganic anions in DDH. The higher levels of sulphate found in infants with DDH suggests either enhanced intake from milk, increased endogenous formation, or impaired renal reabsorption. This investigation demonstrates the power of urine metabolomics and highlights the importance of normalisation for hydration status to disentangle developmental disorders. Our results strongly suggest that DDH is a systemic disease associated with altered uptake, formation, or handling of sulphate. There is potential for new opportunities in the prevention or treatment of DDH via nutritional intervention.
Differences in Properties between Hybrid Wire Arc Additive-Milling Subtractive Manufactured Aluminum and Magnesium Alloys
Authors: Zhang Shuai , Gong Mengcheng , Cen Ling , Lu Yang , Gao Ming
Published: February 2023

Hybrid wire arc additive-milling subtractive manufacturing (HWMM) is an effective way to improve the quality of complex metal components, but the difference in the properties of the aluminum alloy and magnesium alloy fabricated by HWMM has been not addressed. In the paper, the differences in deposition accuracy and tensile anisotropy between the Al5Si Al and AZ31B Mg alloys were studied by using the HWMM method. Under the optimal parameters, the minimum surface roughness of the AZ31B sample was 146.1 μm, which was 90% higher than for the Al5Si sample. The differences in the tensile strength and elongation of the AZ31B sample were 32% and 56%, respectively, being 6 and 3.3 times higher than those of the Al5Si samples. According to the fracture behavior of the samples, the tensile anisotropy of both alloys was mainly attributed to defects such as incomplete fusion and porosity in the fusion line. However, there was obvious structural inhomogeneity in AZ31B samples, where the grain size difference between adjacent areas reached 40%. This led to the easier fracture of AZ31B samples. These results contribute to our understanding of the HWMM of light alloys.