bluuebunny/crossref_metadata_2025 huggingface dataset:
Therapeutic Approach of Flavonoid in Ameliorating Diabetic Cardiomyopathy by Targeting Mitochondrial-Induced Oxidative Stress
Authors: Sapian Syaifuzah , Taib Izatus Shima , Latip Jalifah , Katas Haliza , Chin Kok-Yong , Mohd Nor Nor Anizah , Jubaidi Fatin Farhana , Budin Siti Balkis
Published: October 2021

Diabetes cardiomyopathy is one of the key factors of mortality among diabetic patients around the globe. One of the prior contributors to the progression of diabetic cardiomyopathy is cardiac mitochondrial dysfunction. The cardiac mitochondrial dysfunction can induce oxidative stress in cardiomyocytes and was found to be the cause of majority of the heart morphological and dynamical changes in diabetic cardiomyopathy. To slow down the occurrence of diabetic cardiomyopathy, it is crucial to discover therapeutic agents that target mitochondrial-induced oxidative stress. Flavonoid is a plentiful phytochemical in plants that shows a wide range of biological actions against human diseases. Flavonoids have been extensively documented for their ability to protect the heart from diabetic cardiomyopathy. Flavonoids’ ability to alleviate diabetic cardiomyopathy is primarily attributed to their antioxidant properties. In this review, we present the mechanisms involved in flavonoid therapies in ameliorating mitochondrial-induced oxidative stress in diabetic cardiomyopathy.
Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
Authors: Klas Katharina , Copic Dragan , Direder Martin , Laggner Maria , Prucksamas Patricia Sandee , Gruber Florian , Ankersmit Hendrik Jan , Mildner Michael
Published: October 2021

Sebaceous glands are adnexal structures, which critically contribute to skin homeostasis and the establishment of a functional epidermal barrier. Sebocytes, the main cell population found within the sebaceous glands, are highly specialized lipid-producing cells. Sebaceous gland-resembling tissue structures are also found in male rodents in the form of preputial glands. Similar to sebaceous glands, they are composed of lipid-specialized sebocytes. Due to a lack of adequate organ culture models for skin sebaceous glands and the fact that preputial glands are much larger and easier to handle, previous studies used preputial glands as a model for skin sebaceous glands. Here, we compared both types of sebocytes, using a single-cell RNA sequencing approach, to unravel potential similarities and differences between the two sebocyte populations. In spite of common gene expression patterns due to general lipid-producing properties, we found significant differences in the expression levels of genes encoding enzymes involved in the biogenesis of specialized lipid classes. Specifically, genes critically involved in the mevalonate pathway, including squalene synthase, as well as the sphingolipid salvage pathway, such as ceramide synthase, (acid) sphingomyelinase or acid and alkaline ceramidases, were significantly less expressed by preputial gland sebocytes. Together, our data revealed tissue-specific sebocyte populations, indicating major developmental, functional as well as biosynthetic differences between both glands. The use of preputial glands as a surrogate model to study skin sebaceous glands is therefore limited, and major differences between both glands need to be carefully considered before planning an experiment.
Investigating Machine Learning & Natural Language Processing Techniques Applied for Predicting Depression Disorder from Online Support Forums: A Systematic Literature Review
Authors: Nanomi Arachchige Isuri Anuradha , Sandanapitchai Priyadharshany , Weerasinghe Ruvan
Published: October 2021

Depression is a common mental health disorder that affects an individual’s moods, thought processes and behaviours negatively, and disrupts one’s ability to function optimally. In most cases, people with depression try to hide their symptoms and refrain from obtaining professional help due to the stigma related to mental health. The digital footprint we all leave behind, particularly in online support forums, provides a window for clinicians to observe and assess such behaviour in order to make potential mental health diagnoses. Natural language processing (NLP) and Machine learning (ML) techniques are able to bridge the existing gaps in converting language to a machine-understandable format in order to facilitate this. Our objective is to undertake a systematic review of the literature on NLP and ML approaches used for depression identification on Online Support Forums (OSF). A systematic search was performed to identify articles that examined ML and NLP techniques to identify depression disorder from OSF. Articles were selected according to the PRISMA workflow. For the purpose of the review, 29 articles were selected and analysed. From this systematic review, we further analyse which combination of features extracted from NLP and ML techniques are effective and scalable for state-of-the-art Depression Identification. We conclude by addressing some open issues that currently limit real-world implementation of such systems and point to future directions to this end.
Passive Fault-Tolerant Control of a 2-DOF Robotic Helicopter
Authors: Zuñiga Manuel A. , Ramírez Luis A. , Romero Gerardo , Alcorta-García Efraín , Arceo Alejandro
Published: October 2021

The presence of faults in dynamic systems causes the potential loss of some of the control objectives. For that reason, a fault-tolerant controller is required to ensure a proper operation, as well as to reduce the risk of accidents. The present work proposes a passive fault-tolerant controller that is based on robust techniques, which are utilized to adjust a proportional-derivative scheme through a linear matrix inequality. In addition, a nonlinear term is included to improve the accuracy of the control task. The proposed methodology is implemented in the control of a two degrees of a freedom robotic helicopter in a simulation environment, where abrupt faults in the actuators are considered. Finally, the proposed scheme is also tested experimentally in the Quanser® 2-DOF Helicopter, highlighting the effectiveness of the proposed controller.
Time-Optimal Gathering under Limited Visibility with One-Axis Agreement
Authors: Poudel Pavan , Sharma Gokarna
Published: October 2021

We consider the distributed setting of N autonomous mobile robots that operate in Look-Compute-Move (LCM) cycles following the well-celebrated classic oblivious robots model. We study the fundamental problem of gathering N autonomous robots on a plane, which requires all robots to meet at a single point (or to position within a small area) that is not known beforehand. We consider limited visibility under which robots are only able to see other robots up to a constant Euclidean distance and focus on the time complexity of gathering by robots under limited visibility. There exists an O(DG) time algorithm for this problem in the fully synchronous setting, assuming that the robots agree on one coordinate axis (say north), where DG is the diameter of the visibility graph of the initial configuration. In this article, we provide the first O(DE) time algorithm for this problem in the asynchronous setting under the same assumption of robots’ agreement with one coordinate axis, where DE is the Euclidean distance between farthest-pair of robots in the initial configuration. The runtime of our algorithm is a significant improvement since for any initial configuration of N≥1 robots, DE≤DG, and there exist initial configurations for which DG can be quadratic on DE, i.e., DG=Θ(DE2). Moreover, our algorithm is asymptotically time-optimal since the trivial time lower bound for this problem is Ω(DE).
Species Complex and Temporal Associations between Coccinellids and Aphids in Alfalfa Stands in Spain
Authors: Meseguer Roberto , Levi-Mourao Alexandre , Pons Xavier
Published: October 2021

Alfalfa is known to be an important reservoir harboring natural enemies. The reduction in insecticide sprayings in recent years has allowed us to study the coccinellid species complex in this crop and the relationship between these predators and aphids. Alfalfa was sampled by sweep-netting throughout its productive period in several commercial stands each year between 2010 and 2021. The numbers and species of aphids and coccinellids were recorded. Sixteen coccinellid species were found. Coccinella septempunctata and Hippodamia variegata were, by far, the most prevalent species, with the former dominating during the first and second intercuts, whereas the latter dominated from the third to the fifth intercut. Acyrthosiphon pisum and Therioaphis trifolii were the most abundant aphid species, peaking in the second and fourth intercuts, respectively. Positive correlations were found between the abundance of C. septempunctata and A. pisum at the second intercut, between H. variegata and T. trifolii at the fourth intercut, and between H. variegata and the total number of aphids in the fifth intercut. This study helps to increase the knowledge on the predator–prey relationships of this crop and allows for designing strategies of conservation biological control against aphids.
Study of Resistance to Disturbances of the Main Types of Communication Systems on Board Military Ships Used during Interception or Search and Rescue Missions
Authors: Solcanu Vasile , Gaiceanu Marian , Rosu Georgiana
Published: October 2021

In addition to combat missions, military ships often participate in search-and-rescue missions or interception of ships with refugees or migrants. The communication systems and modes of work that may be used during these missions will be in accordance with the International Convention for the Safety of Life at Sea (SOLAS). This paper aims to demonstrate by theoretical methods (analytical and numerical) the noise stability of communication systems using analog modulation in high-noise conditions, characteristic of the marine environment. The stability of analog systems employing amplitude (AM), frequency (FM), and phase (PM) modulations is investigated. The analyzed systems are currently under use in distress maritime communications.
Moderate and High Disease Activity Predicts the Development of Carotid Plaque in Rheumatoid Arthritis Patients without Classic Cardiovascular Risk Factors: Six Years Follow-Up Study
Authors: Ferraz-Amaro Iván , Corrales Alfonso , Atienza-Mateo Belén , Vegas-Revenga Nuria , Prieto-Peña Diana , Blanco Ricardo , González-Gay Miguel Á.
Published: October 2021

Patients with rheumatoid arthritis (RA) have a higher incidence of subclinical atherosclerosis and cardiovascular (CV) disease. It is postulated that the appearance of accelerated atherosclerosis in these patients is a consequence of the inflammation present in the disease. In this study, we aim to determine if baseline disease activity in patients with RA predicts the future development of carotid plaque. A set of consecutive RA patients without a history of CV events, cancer or chronic kidney disease, who did not show carotid plaque in a carotid ultrasound assessment, were prospectively followed up for at least 5 years. At the time of recruitment, CV risk factors and disease-related data, including disease activity scores, were assessed. At the end of the follow-up, a carotid ultrasound was repeated and patients were divided into two groups; those who developed carotid plaque, and those who did not. A multivariable regression analysis was performed to define the predictors for the development of carotid plaque. One hundred and sixty patients with RA were followed up for an average of 6 ± 1 years. After this time, 66 (41%) of the patients had developed carotid plaque, and 94 (59%) did not. Patients with carotid plaque were significantly older (47 ± 13 vs. 55 ± 9 years, p < 0.001) at baseline, were more frequently diabetic (0% vs. 6%, p = 0.028), and had higher total cholesterol (197 ± 36 vs. 214 ± 40 mg/dL, p = 0.004) and LDL cholesterol (114 ± 35 vs. 126 ± 35 mg/dL, p = 0.037) at the beginning of the study. After multivariable adjustment, patients who were in the moderate and high disease activity (DAS28-CRP) categories displayed a higher odds ratio for the appearance of carotid plaque (OR 2.26 [95% CI 1.02–5.00], p = 0.044) compared to those in the DAS-28-CRP remission category. Remarkably, when patients were divided in patients within the low-risk SCORE category, and patients included in the remaining SCORE categories (moderate, high and very high), the relation between DAS28-CRP and the development of carotid plaque was only significant in the low-risk SCORE category. In conclusion, disease activity predicts the future development of subclinical atherosclerosis in patients with RA.
Transcatheter Arterial Embolization in Acute Non-Variceal Gastrointestinal Bleedings: A Ten-Year Single-Center Experience in 91 Patients and Review of the Literature
Authors: Fontana Federico , Piacentino Filippo , Ossola Christian , Coppola Andrea , Curti Marco , Macchi Edoardo , De Marchi Giuseppe , Floridi Chiara , Ierardi Anna Maria , Carrafiello Gianpaolo , Segato Sergio , Carcano Giulio , Venturini Massimo
Published: October 2021

Objective: To report the safety and efficacy of trans-arterial embolization (TAE) for upper gastrointestinal bleeding (UGIB) and lower gastrointestinal bleeding (LGIB) due to different etiologies in 91 patients for ten years. Methods: A retrospective analysis of GIB treated between January 2010 and December 2020 was performed. TAE was performed using different embolic agents (coils, particles, glue, gelatin sponge, and EVOH-based agents). Technical success, secondary technical success, clinical success, and complications were evaluated. Results: Technical success was achieved in 74/91 (81.32%) patients. Seventeen patients (18.68%) required re-intervention. Secondary technical success was achieved in all cases (100.0%). Clinical success was achieved in 81/91 patients (89.01%). No major complications were recorded; overall, minor complications occurred in 20/91 patients. Conclusions: TAE is a technically feasible and safe therapeutic option for patients with GIB from a known or blind anatomic source where endoscopic therapy has failed or is deemed unfeasible.
Ultrasound Cyclo Plasty for Treatment of Surgery-Naïve Open-Angle Glaucoma Patients: A Prospective, Multicenter, 2-Year Follow-Up Trial
Authors: Figus Michele , Posarelli Chiara , Nardi Marco , Stalmans Ingeborg , Vandewalle Evelien , Melamed Shlomo , Skaat Alon , Leshno Ari , Sousa David Cordeiro , Pinto Luis Abegão
Published: October 2021

Background: The purpose of this prospective study was to evaluate the efficacy and safety of the Ultrasound Cyclo Plasty (UCP) procedure using high-intensity focused ultrasound in surgery-naïve open-angle glaucoma patients. Methods: prospective, non-randomized, single-arm, multicenter clinical trial. Sixty-six eyes with primary open-angle glaucoma, intraocular pressure (IOP) ≥21 mmHg and with no history of filtering surgery were enrolled. Patients were treated by UCP with a therapy probe comprising six piezoelectric transducers, consecutively activated for 8 s each. Complete ophthalmic examination was performed before the procedure, 1 day after the procedure, and 1, 3, 6, 12, 18 and 24 months after the procedure. Primary outcomes were complete success (defined as IOP lowering from baseline ≥20% without additional glaucoma medications) and vision-threatening complications. Secondary outcomes were the presence of complications and the reduction of the number of medications used. Results: IOP was significantly reduced after one procedure (p < 0.05), from a mean pre-operative value of 24.3 ± 2.9 mmHg (n = 2.3 hypotensive medications) to a mean value of 15.9 ± 3.6 mmHg (n = 2.2 hypotensive medications) at 2 years (mean IOP lowering of 33%). Surgical success was achieved in 74% of eyes. Notwithstanding side effects such as transient anterior chamber inflammation, refractive error changes, transient hypotony and macular edema, no major intra or post-operative complications such as phthisis, induced cataract, neovascularization or significant vision loss were observed. Conclusions: Ultrasound Cyclo Plasty is a valuable, effective and well-tolerated procedure to lower IOP in patients with open-angle glaucoma without previous filtering surgery.
Predicting the Exception—CRP and Primary Hip Arthroplasty
Authors: Meier Marc-Pascal , Bauer Ina Juliana , Maheshwari Arvind K. , Husen Martin , Jäckle Katharina , Hubert Jan , Hawellek Thelonius , Lehmann Wolfgang , Saul Dominik
Published: October 2021

Background: While primary hip arthroplasty is the most common operative procedure in orthopedic surgery, a periprosthetic joint infection is its most severe complication. Early detection and prediction are crucial. In this study, we aimed to determine the value of postoperative C-reactive protein (CRP) and develop a formula to predict this rare, but devastating complication. Methods: We retrospectively evaluated 708 patients with primary hip arthroplasty. CRP, white blood cell count (WBC), and several patient characteristics were assessed for 20 days following the operative procedure. Results: Eight patients suffered an early acute periprosthetic infection. The maximum CRP predicted an infection with a sensitivity and specificity of 75% and 56.9%, respectively, while a binary logistic regression reached values of 75% and 80%. A multinominal logistic regression, however, was able to predict an early infection with a sensitivity and specificity of 87.5% and 78.9%. With a one-phase decay, 71.6% of the postoperative CRP-variance could be predicted. Conclusion: To predict early acute periprosthetic joint infection after primary hip arthroplasty, a multinominal logistic regression is the most promising approach. Including five parameters, an early infection can be predicted on day 5 after the operative procedure with 87.5% sensitivity, while it can be excluded with 78.9% specificity.
DNA Methylation Changes in Fibromyalgia Suggest the Role of the Immune-Inflammatory Response and Central Sensitization
Authors: Gerra Maria Carla , Carnevali Davide , Ossola Paolo , González-Villar Alberto , Pedersen Inge Søkilde , Triñanes Yolanda , Donnini Claudia , Manfredini Matteo , Arendt-Nielsen Lars , Carrillo-de-la-Peña Maria Teresa
Published: October 2021

Fibromyalgia (FM) has been explained as a result of gene-environment interactions. The present study aims to verify DNA methylation differences in eleven candidate genome regions previously associated to FM, evaluating DNA methylation patterns as potential disease biomarkers. DNA methylation was analyzed through bisulfite sequencing, comparing 42 FM women and their 42 healthy sisters. The associations between the level of methylation in these regions were further explored through a network analysis. Lastly, a logistic regression model investigated the regions potentially associated with FM, when controlling for sociodemographic variables and depressive symptoms. The analysis highlighted significant differences in the GCSAML region methylation between patients and controls. Moreover, seventeen single CpGs, belonging to other genes, were significantly different, however, only one cytosine related to GCSAML survived the correction for multiple comparisons. The network structure of methylation sites was different for each group; GRM2 methylation represented a central node only for FM patients. Logistic regression revealed that depressive symptoms and DNA methylation in the GRM2 region were significantly associated with FM risk. Our study encourages better exploration of GCSAML and GRM2 functions and their possible role in FM affecting immune, inflammatory response, and central sensitization of pain.
A Quality Improvement Project to Implement Choking Prevention and First Aid Education in Prader–Willi Syndrome Caregivers
Authors: Obrynba Kathryn S. , Anglin Kathryn , Moffett Amy , Steinke Tracie , Kamboj Manmohan K.
Published: October 2021

Prader–Willi syndrome (PWS) is a complex neurodevelopmental genetic disorder characterized by hypotonia and hyperphagia. Consequently, individuals with PWS are at high risk of choking, and choking is a leading cause of morbidity and mortality. The aim of this quality improvement (QI) project is to provide choking prevention and first aid education from 0% to 80% of PWS caregivers seen in a multidisciplinary PWS clinic, and to assess the effectiveness of this education program. A QI initiative was developed to standardize and implement choking prevention and first aid education for PWS caregivers. Using a Likert scale, pre- and post-education assessments were conducted to measure caregiver (1) awareness of the PWS choking risk, (2) self-reported knowledge of choking prevention strategies, and (3) comfort in providing choking first aid. The American Heart Association Family and Friends® CPR (Dallas, TX, USA) curriculum was utilized. Education was provided during a regularly scheduled PWS clinic appointment. At project conclusion, 45/52 (87%) of PWS caregivers received education. A post-education assessment revealed an improvement in PWS caregivers’ awareness of choking risk, self-reported knowledge of choking prevention strategies, and comfort in providing choking first aid. This QI project supports a practice change to implement choking prevention and first aid education as standard process within our PWS clinic.
High Incidence of Cataracts in the Follow-Up of Patients Undergoing Percutaneous Coronary Intervention for Chronic Coronary Total Occlusion
Authors: Rivera-López Ricardo , García-López Celia , Sánchez-Moreno José M. , Rivera-López Rafael A. , Almansa-López Julio , Rivera-Fernández Ricardo , Molina-Navarro Eduardo , Jiménez-Fernández Miriam , Ortiz-Pérez Santiago , Ramírez-Hernández José A.
Published: October 2021

Development of cataracts is a well-known adverse effect of ionizing radiation, but little information is available on their incidence in patients after other medical procedures, such as cardiac catheterizations. The study objective was to determine the incidence of cataracts in a cohort of patients undergoing percutaneous coronary intervention (PCI) for chronic coronary total occlusion (CTO) and its association with radiation dose. The study analyzed the incidence of cataracts during the follow-up of 126 patients who underwent chronic total coronary PCI, using Cox regression to identify predictive factors of cataract development. The study included 126 patients, 86.9% male, with a mean age of 60.5 years (range, 55.0–68.0 years). Twenty-three (18.2% n = 23) developed cataracts during a mean follow-up of 49.5 months (range 37.3–64.5 months). A higher incidence was observed in patients who received more than 5 Gy (29.0% vs. 14.7%, Hazard ratio (HR = 2.84 [1.19–6.77]). Multivariate analysis revealed a relationship between cataract development during the follow-up and a receipt of radiation dose >5 Gy (HR = 2.60, 95% confidence interval [CI 1.03–6.61]; p = 0.03), presence or history of predisposing eye disease (HR = 4.42, CI:1.57–12.40), diabetes (HR = 3.33 [1.22–9.24]), and older age, as in >57 (HR, 6.40 [1.81–22.61]). An elevated incidence of cataracts was observed in patients after PCI for CTO. The onset of cataracts is related to the radiation dose during catheterization, which is a potentially avoidable effect of which operators should be aware.
Risk Factors for Revolving Door in Children and Adolescents with Psychiatric Disorders
Authors: D’Aiello Barbara , Menghini Deny , Averna Roberto , Labonia Milena , Vicari Stefano
Published: October 2021

Revolving Door (RD) is a frequent phenomenon afflicting children and adolescents with psychiatric diagnoses. Nevertheless, risk factors for RD are still a matter of debate. To better understand RD phenomenon, we conducted a retrospective study on 224 children and adolescents (165 females and 59 males, aged 6–16 years) with a psychiatric hospitalization, taking the multiple risk factors together. At this aim, 108 patients with multiple hospitalizations and 116 patients with only one hospitalization were compared on demographic characteristics, clinical conditions, psychiatric ward stay, and post-discharge management factors. More than half of psychiatric patients were readmitted within three months of discharge. RD patients presented greater severity of illness, needed longer stays, and were more frequently placed in residential facilities than non-RD patients. Non-suicidal self-injurious and adoption were the main predictors of RD. Clinical instruments that detected behavioural and emotional symptoms, suicidal ideation severity, and level of impairment of the person’s functioning were useful to identify patients at high risk for RD. In conclusion, our findings pointed out that several risk factors have to be considered to better understand and, in the future, prevent RD phenomenon.
Comparing Masticatory Performance of Maxillary Mini Dental Implant Overdentures, Complete Removable Dentures and Dentate Subjects
Authors: Van Doorne Luc , De Backer Ben , Matthys Carine , De Bruyn Hugo , Vandeweghe Stefan
Published: October 2021

Background: Mini dental implant (MDI) overdenture rehabilitation for the edentulous maxilla is a valuable, less invasive and affordable treatment alternative for complete removable dentures (CRD). However, comparative quantification of masticatory performance in different oral conditions are scarce. Purpose: This study compares objective masticatory performance of dentate groups, maxillary CRD and MDI overdentures and subjective masticatory performance in maxillary CRD and MDI overdentures. Materials and Methods: Four groups were defined, age 20+ dentate dental students (DS), age 50+ complete dentate subjects (DP), age 50+ maxillary CRD or MDI overdentures/dentate mandible. Objective masticatory performance was evaluated by measuring circular Variance of Hue (VOH) or the mixture degree of two-color chewing gum (Hue-check View Gum® Test). Additionally, subjective masticatory comparison was investigated in the CRD and MDI groups, with a visual analogue scale (VAS) for different food consistencies and the Oral Health Related Quality of Life (OHRQL) OHIP-14 questionnaire. Results: The mean VOH was 0.11 (SD 0.50, range 0.05–0.27) for the dentate dental 20+ students, 0.13 (SD 0.08, range 0.03–0.31) for the 50+ dentate group (p = 0.774), 0.41 (SD 0.41, range 0.14–0.76) for the CRD group and 0.39 (SD 0.18, range 0.07–0.76) for the MDI group (p = 0.725). Based on the VAS scores, no improvement was found between the CRD and the MDI overdenture group (p > 0.050). The mean OHIP-14 total score was 12.10 (SD 15.87, range 0–56) for CRD, while the MDI group (p = 0.039) saw a significant improvement to 2.85 (SD 2.85, range 0–15). Conclusion: Comparable results in objective masticatory performance were registered in dentate 20+ and 50+ subjects with a remarkable inferior outcome for the CRD and MDI group. Compared to CRD, MDI overdentures revealed no substantial improvement in subjective and objective masticatory performance. However, for MDI a significant increase in OHRQL was apparent.
Molecular Mechanisms of 5-Fluorocytosine Resistance in Yeasts and Filamentous Fungi
Authors: Delma Fatima Zohra , Al-Hatmi Abdullah M. S. , Brüggemann Roger J. M. , Melchers Willem J. G. , de Hoog Sybren , Verweij Paul E. , Buil Jochem B.
Published: October 2021

Effective management and treatment of fungal diseases is hampered by poor diagnosis, limited options for antifungal therapy, and the emergence of antifungal drug resistance. An understanding of molecular mechanisms contributing to resistance is essential to optimize the efficacy of currently available antifungals. In this perspective, one of the oldest antifungals, 5-fluorocytosine (5-FC), has been the focus of recent studies applying advanced genomic and transcriptomic techniques to decipher the order of events at the molecular level that lead to resistance. These studies have highlighted the complexity of resistance and provided new insights that are reviewed in the present paper.
Stimulated Expression of CXCL12 in Adrenocortical Carcinoma by the PPARgamma Ligand Rosiglitazone Impairs Cancer Progression
Authors: Cantini Giulia , Fei Laura , Canu Letizia , Lazzeri Elena , Sottili Mariangela , Francalanci Michela , Angelotti Maria Lucia , De Filpo Giuseppina , Ercolino Tonino , Gelmini Stefania , Mangoni Monica , Nesi Gabriella , Hantel Constanze , Mannelli Massimo , Maggi Mario , Luconi Michaela
Published: October 2021

Adrenocortical carcinoma (ACC) is a rare malignancy with poor prognosis when metastatic and scarce treatment options in the advanced stages. In solid tumors, the chemokine CXCL12/CXCR4 axis is involved in the metastatic process. We demonstrated that the human adrenocortex expressed CXCL12 and its cognate receptors CXCR4 and CXCR7, not only in physiological conditions, but also in ACC, where the receptors’ expression was higher and the CXCL12 expression was lower than in the physiological conditions. In a small pilot cohort of 22 ACC patients, CXCL12 negatively correlated with tumor size, stage, Weiss score, necrosis, and mitotic activity. In a Kaplan–Meier analysis, the CXCL12 tumor expression significantly predicted disease-free, progression-free, and overall survival. In vitro treatment of the primary ACC H295R and of the metastatic MUC-1 cell line with the PPARγ-ligand rosiglitazone (RGZ) dose-dependently reduced proliferation, resulting in a significant increase in CXCL12 and a decrease in its receptors in the H295R cells only, with no effect on the MUC-1 levels. In ACC mouse xenografts, tumor growth was inhibited by the RGZ treatment before tumor development (prevention-setting) and once the tumor had grown (therapeutic-setting), similarly to mitotane (MTT). This inhibition was associated with a significant suppression of the tumor CXCR4/CXCR7 and the stimulation of human CXCL12 expression. Tumor growth correlated inversely with CXCL12 and positively with CXCR4 expression, suggesting that local CXCL12 may impair the primary tumor cell response to the ligand gradient that may contribute to driving the tumor progression. These findings indicate that CXCL12/CXCR4 may constitute a potential target for anti-cancer agents such as rosiglitazone in the treatment of ACC.
Influence of Catheter–Incision Congruency in Epidural Analgesia on Postcesarean Pain Management: A Single-Blinded Randomized Controlled Trial
Authors: Chen Ying-Hsi , Chou Wei-Han , Yie Jr-Chi , Teng Hsiao-Chun , Wu Yi-Luen , Wu Chun-Yu
Published: October 2021

Patient-controlled epidural analgesia (PCEA) or epidural morphine may alleviate postcesarean pain; however, conventional lumbar epidural insertion is catheter–incision incongruent for cesarean delivery. Methods: In total, 189 women who underwent cesarean delivery were randomly divided into four groups (low thoracic PCEA, lumbar PCEA, low thoracic morphine, and lumbar morphine groups) for postcesarean pain management. Pain intensities, including static pain, dynamic pain, and uterine cramp, were measured using a 100 mm visual analog scale (VAS). The proportion of participants who experienced dynamic wound pain with a VAS score of >33 mm was evaluated as the primary outcome. Adverse effects, including lower extremity blockade, pruritus, postoperative nausea and vomiting, sedation, and time of first passage of flatulence, were evaluated. Results: The low thoracic PCEA group had the lowest proportion of participants reporting dynamic pain at 6 h after spinal anesthesia (low thoracic PCEA, 28.8%; lumbar PCEA, 69.4%; low thoracic morphine, 67.3%; lumbar morphine group, 73.9%; p < 0.001). The aforementioned group also reported the most favorable VAS scores for static, dynamic, and uterine cramp pain during the first 24 h after surgery. Adverse effect profiles were similar among the four groups, but a higher proportion of participants in the lumbar PCEA group (approximately 20% more than in the other three groups) reported prolonged postoperative lower extremity motor blockade (p = 0.005). In addition, the first passage of flatulence after surgery reported by the low thoracic PCEA group was approximately 8 h earlier than that of the two morphine groups (p < 0.001). Conclusions: Epidural congruency is essential to PCEA for postcesarean pain. Low thoracic PCEA achieves favorable analgesic effects and may promote postoperative gastrointestinal recovery without additional adverse effects.
More Haste, Less Speed: How Update Frequency of Mobile Apps Influences Consumer Interest
Authors: Gong Xuan , Razzaq Amar , Wang Wei
Published: October 2021

The present study proposes a theoretical framework that uncovers the joint effects of the update frequency of apps and product type of the update on consumer interest and its underlying mechanisms. Building on the theory of mental accounting and regulatory focus, we propose that the effects of update frequency on consumer interest are different for hedonic products and utilitarian products. The authors give insights into the main effects with an empirical analysis of a field data set and establish an understanding of the fundamental mechanisms by two laboratory experiments. The findings show that for hedonic products, high update frequency contributes to higher consumer interest by affecting the benefit perception of consumers. For utilitarian products, low update frequency results in higher consumer interest by influencing the risk perception of consumers. Furthermore, the level of update can affect the combined effects of product type and update frequency on consumer interest and, particularly for low update levels, the aforementioned association can be reversed.
Spatial Diffusion of E-Commerce in China’s Counties: Based on the Perspective of Regional Inequality
Authors: Wang Fan , Wang Mingfeng , Yuan Shichen
Published: October 2021

In recent decades, China has been on a new journey toward a digital economy of which e-commerce accounts for a substantial proportion. Despite some controversy, the innovation diffusion hypothesis and efficiency hypothesis of online shopping have been tested in research on the urban–rural dual structure. However, research on the spatial diffusion model of online business is sparse. Based on the online business and online shopping index released by the Ali Research Institute, this article compares the spatial diffusion model of online shopping and online business in the core–periphery structure based on the inequality between the eastern and western regions of China. Our study suggests that online business trends are in line only with the innovation diffusion hypothesis, with marginal counties having lower levels of online business. Online shopping, on the other hand, is in line with the innovation diffusion hypothesis and partially with the efficiency hypothesis, with a higher index of online shopping in the core regions and some peripheral counties. The discrepancy in the spatial diffusion mode is due to the differences in aims and supporting elements between online business and online shopping. Apart from infrastructure, the diffusion of online business is largely constrained by the regional industrial base, while online shopping is influenced by income and savings levels, which is the main reason for the differences in the spatial diffusion of online business and online shopping. We argue that the diffusion of online business has not led to the ability to balance regional inequalities at the national scale, while online shopping has the potential to bridge core and peripheral disparities better than online businesses, not in terms of the ability to bridge economic disparities, but in terms of the potential to reduce spatial consumption inequalities and welfare gains.
Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
Authors: Buchbender Mayte , Lugenbühl Amelie , Fehlhofer Jakob , Kirschneck Christian , Ries Jutta , Lutz Rainer , Sticherling Michael , Kesting Marco Rainer
Published: October 2021

Background: Systemic scleroderma (SSc) has multiple orofacial effects. The aim of this study was to analyze the expression of inflammatory mediators in biofilm samples. It was hypothesized that different expression levels and clinical associations might be drawn. Methods: A total of 39 biofilm samples from group 1 = SSc and group 2 = healthy control were examined for the expression levels of interleukin (IL)-2,-6, and -10; matrix metalloprotease (MMP)-9; and surface antigens CD90 and CD34 by quantitative real-time PCR and clinical parameters. Relative quantitative (RQ) gene expression was determined using the ∆∆CT method. Results: The mean bleeding on probing values (p = 0.006), clinical attachment loss (CAL) (p = 0.009), gingival recession (p = 0.020), limited mouth opening (p = 0.001) and cervical tooth defects (p = 0.011) were significantly higher in group 1. RQ expressions of IL-2 and CD34 were significantly lower, IL-6, MMP-9, and CD90 were significantly higher. There was a significant positive correlation of IL-6/MMP-9 and negative correlation of mouth opening/CAL and IL-6/CAL. Conclusion: Different expression levels of IL-2, IL-6, MMP-9, CD34 and CD90 were detected in biofilm samples from patients with SSc compared to control. An immunological correlation to the clinical parameters of mouth opening and CAL was shown; thus, we conclude that SSc might have an impact on periodontal tissues.
A Multiallelic Molecular Beacon-Based Real-Time RT-PCR Assay for the Detection of SARS-CoV-2
Authors: Chrysostomou Andreas C. , Hezka Rodosthenous Johana , Topcu Cicek , Papa Christina , Aristokleous Antonia , Stathi Georgia , Christodoulou Christina , Eleftheriou Christina , Stylianou Dora C. , Kostrikis Leondios G.
Published: October 2021

Emerging infectious viruses have led to global advances in the development of specific and sensitive detection techniques. Viruses have an inherent potential to easily mutate, presenting major hurdles for diagnostics and requiring methods capable of detecting genetically diverse viral strains. One such infectious agent is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in December 2019 and has resulted in the global coronavirus disease 2019 (COVID-19) pandemic. This study presents a real-time reverse transcription PCR (RT-PCR) detection assay for SARS-CoV-2, taking into account its intrinsic polymorphic nature that arises due to genetic drift and recombination, as well as the possibility of continuous and multiple introductions of genetically nonidentical strains into the human population. This advance was achieved by using mismatch-tolerant molecular beacons designed to specifically detect the SARS-CoV-2 S, E, M, and N genes. These were applied to create a simple and reproducible real-time RT-PCR assay, which was validated using external quality control panels (QCMD: CVOP20, WHO: SARS-CoV-2-EQAP-01) and clinical samples. This assay was designed for high target detection accuracy and specificity and can also be readily adapted for the detection of other emerging and rapidly mutating pathogens.
Lateral Ridge Augmentation with Guided Bone Regeneration Using Particulate Bone Substitutes and Injectable Platelet-Rich Fibrin in a Digital Workflow: 6 Month Results of a Prospective Cohort Study Based on Cone-Beam Computed Tomography Data
Authors: Wang Maoxia , Zhang Xiaoqing , Li Yazhen , Mo Anchun
Published: October 2021

This study aimed to test whether or not a digital workflow for GBR with particulate bone substitutes and injectable platelet-rich fibrin improved the thickness of the hard tissue compared to the conventional workflow. 26 patients in need of lateral bone augmentation were enrolled. GBR with particulate bone substitutes and injectable platelet-rich fibrin was performed in all patients. Patients were divided into two groups: control (conventional workflow; n = 14) and test (digital workflow; n = 12). CBCT scans were performed before surgery, immediately after wound closure, and 6 months post-surgery, and the labial thickness of the hard tissue (LT) was assessed at 0–5 mm apical to the implant shoulder (LT0–LT5) at each time point. A total of 26 patients were included in this study. After wound closure, the test group showed significantly greater thickness in LT0–LT2 than the control group (LT0: test: 4.31 ± 0.73 mm, control: 2.99 ± 1.02 mm; LT1: test: 4.55 ± 0.69 mm, control: 3.60 ± 0.96 mm; LT2: test: 4.76 ± 0.54 mm, control: 4.05 ± 1.01 mm; p < 0.05). At 6 months, significant differences in LT0–LT1 were detected between the groups (LT0: test: 1.88 ± 0.57 mm, control: 1.08 ± 0.60 mm; LT1: test: 2.36 ± 0.66 mm, control: 1.69 ± 0.58 mm; p < 0.05). Within the limitations of this study, the use of digital workflow in GBR with particulate bone substitutes and i-PRF exerted a positive effect on the labial thickness of hard tissue in the coronal portion of the implant after wound closure and at 6 months.
Smart Self-Healing Capability of Asphalt Material Using Bionic Microvascular Containing Oily Rejuvenator
Authors: Yang Peng , Wang Li-Qing , Gao Xu , Wang Sai , Su Jun-Feng
Published: October 2021

It has become one of the research directions of intelligent materials for self-healing asphalt pavements to use a bionic microvascular containing oily rejuvenator. The rejuvenator in a microvascular can carry out the healing of asphalt micro-cracks, thus reducing the damage to and prolonging the life of asphalt pavement. The aim of this work was to investigate the smart self-healing capability of an asphalt/microvascular material through its microstructure and mechanical properties. Microstructure observation indicated no interface separation between the microvasculars and bitumen matrix. Micro-CT images showed that microvasculars dispersed in asphalt samples without accumulation or tangles. The phenomenon of microcracks healing without intervention was observed, which proved that the fractured asphalt sample carried out the self-healing process with the help of rejuvenator diffusing out from the broken microvasculars. The self-healing efficiency of asphalt samples was also evaluated through a tensile test considering the factors of microvasculars content, healing time and healing temperature. It was found that the tensile strength of the asphalt samples was greatly enhanced by the addition of microvasculars under a set test condition. Self-healing efficiency was enhanced with more broken microvasculars in the rupture interface of the asphalt sample. During two self-healing cycles, the self-healing efficiency of the asphalt sample with three microvascular per 1 cm2 of a broken interface were able to reach 80% and 86%. This proves that microvasculars containing rejuvenator play a practical role in the self-healing process of asphalt. With an increase in temperature from 0 to 30 °C, the self-healing capability of the asphalt samples increased dramatically. An increase in time increased the self-healing capability of the bitumen samples. At last, a preliminary mathematical model also deduced that the self-healing efficiency was determined by the individual healing steps, including release, penetration and diffusion of the rejuvenator agent.
Activated Carbon Derived from Carbonization of Kevlar Waste Materials: A Novel Single Stage Method
Authors: Karthik Daniel , Baheti Vijay , Militky Jiri , Naeem Muhammad Salman , Tunakova Veronika , Ali Azam
Published: October 2021

The augmented demands of textile materials over time have brought challenges in the disposal of substantial volumes of waste generated during the processing and end of life of such materials. Taking into consideration environmental safety due to discarding of textile waste, it becomes critical to recuperate useful products from such waste for economic reasons. The present work deals with the preparation of porous and electrically conductive activated carbon fabric by a novel single stage method of simultaneous carbonization and physical activation of Kevlar feedstock material procured from local industries, for effective electromagnetic (EM) shielding applications. The Kevlar fabric waste was directly carbonized under a layer of charcoal without any intermediate stabilization step at 800 °C, 1000 °C, and 1200 °C, with a heating rate of 300 °C/h and without any holding time. The physical and morphological properties of the activated carbon, influenced by carbonization process parameters, were characterized from EDX, X-ray diffraction, SEM analysis, and BET analysis. Furthermore, the electrical conductivity was analyzed. Finally, the potential application of the activated material for EM shielding effectiveness was analyzed at low (below 1.5 GHz) and high (2.45 GHz) frequencies. The phenomena of multiple internal reflections and absorption of electromagnetic radiations was found dominant in the case of activated carbon fabric produced at higher carbonization temperatures.
Original Approach to Synthesize TiO2/ZnO Hybrid Nanosponges Used as Photoanodes for Photoelectrochemical Applications
Authors: Navarro-Gázquez Pedro José , Muñoz-Portero Maria José , Blasco-Tamarit Encarna , Sánchez-Tovar Rita , Fernández-Domene Ramon Manuel , García-Antón Jose
Published: October 2021

In the present work, TiO2/ZnO hybrid nanosponges have been synthesized for the first time. First, TiO2 nanosponges were obtained by anodization under hydrodynamic conditions in a glycerol/water/NH4F electrolyte. Next, in order to achieve the anatase phase of TiO2 and improve its photocatalytic behaviour, the samples were annealed at 450 °C for 1 h. Once the TiO2 nanosponges were synthesized, TiO2/ZnO hybrid nanosponges were obtained by electrodeposition of ZnO on TiO2 nanosponges using different temperatures, times, and concentrations of zinc nitrate (Zn(NO3)2). TiO2/ZnO hybrid nanosponges were used as photoanodes in photoelectrochemical water splitting tests. The results indicate that the photoelectrochemical response improves, in the studied range, by increasing the temperature and the Zn(NO3)2 concentration during the electrodeposition process, obtaining an increase in the photoelectrochemical response of 141% for the TiO2/ZnO hybrid nanosponges electrodeposited at 75 °C with 10 mM Zn(NO3)2 for 15 min. Furthermore, morphological, chemical, and structural characterization was performed by Field Emission Scanning Electron Microscopy (FE-SEM) with Energy Dispersive X-Ray spectroscopy (EDX), Raman Confocal Laser Spectroscopy, X-Ray Photoelectron Spectroscopy (XPS), and Grazing Incidence X-Ray Diffraction (GIXRD).
Preparation of Smart Materials by Additive Manufacturing Technologies: A Review
Authors: Mondal Kunal , Tripathy Prabhat Kumar
Published: October 2021

Over the last few decades, advanced manufacturing and additive printing technologies have made incredible inroads into the fields of engineering, transportation, and healthcare. Among additive manufacturing technologies, 3D printing is gradually emerging as a powerful technique owing to a combination of attractive features, such as fast prototyping, fabrication of complex designs/structures, minimization of waste generation, and easy mass customization. Of late, 4D printing has also been initiated, which is the sophisticated version of the 3D printing. It has an extra advantageous feature: retaining shape memory and being able to provide instructions to the printed parts on how to move or adapt under some environmental conditions, such as, water, wind, light, temperature, or other environmental stimuli. This advanced printing utilizes the response of smart manufactured materials, which offer the capability of changing shapes postproduction over application of any forms of energy. The potential application of 4D printing in the biomedical field is huge. Here, the technology could be applied to tissue engineering, medicine, and configuration of smart biomedical devices. Various characteristics of next generation additive printings, namely 3D and 4D printings, and their use in enhancing the manufacturing domain, their development, and some of the applications have been discussed. Special materials with piezoelectric properties and shape-changing characteristics have also been discussed in comparison with conventional material options for additive printing.