bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing Goodness of Fit for Verifying Probabilistic Forecasts
Authors: Kang Tae-Ho , Sharma Ashish , Marshall Lucy
Published: October 2021

The verification of probabilistic forecasts in hydro-climatology is integral to their development, use, and adoption. We propose here a means of utilizing goodness of fit measures for verifying the reliability of probabilistic forecasts. The difficulty in measuring the goodness of fit for a probabilistic prediction or forecast is that predicted probability distributions for a target variable are not stationary in time, meaning one observation alone exists to quantify goodness of fit for each prediction issued. Therefore, we suggest an additional dissociation that can dissociate target information from the other time variant part—the target to be verified in this study is the alignment of observations to the predicted probability distribution. For this dissociation, the probability integral transformation is used. To measure the goodness of fit for the predicted probability distributions, this study uses the root mean squared deviation metric. If the observations after the dissociation can be assumed to be independent, the mean square deviation metric becomes a chi-square test statistic, which enables statistically testing the hypothesis regarding whether the observations are from the same population as the predicted probability distributions. An illustration of our proposed rationale is provided using the multi-model ensemble prediction for El Niño–Southern Oscillation.
Troubleshooting Difficult Bile Duct Access: Advanced ERCP Cannulation Techniques, Percutaneous Biliary Drainage, or EUS-Guided Rendezvous Technique?
Authors: Chan Ting-Ting , Chew Marcus , Tang Raymond
Published: October 2021

Despite experienced hands and availability of various well-designed catheters and wires, selective bile duct cannulation may still fail in 10–20% of cases during endoscopic retrograde cholangiopancreatography (ERCP). In case standard ERCP cannulation technique fails, salvage options include advanced ERCP cannulation techniques such as double-guidewire technique (DGW) with or without pancreatic stenting and precut papillotomy, percutaneous biliary drainage (PBD), and endoscopic ultrasound-guided Rendezvous (EUS-RV) ERCP. If the pancreatic duct is inadvertently entered during cannulation attempts, DGW technique is a reasonable next step, which can be followed by pancreatic stenting to reduce risks of post-ERCP pancreatitis (PEP). Studies suggest that early precut papillotomy is not associated with a higher risk of PEP, while needle-knife fistulotomy is the preferred method. For patients with critical clinical condition who may not be fit for endoscopy, surgically altered anatomy in which endoscopic biliary drainage is not feasible, and non-communicating multisegmental biliary obstruction, PBD has a unique role to provide successful biliary drainage efficiently in this particular population. As endoscopic ultrasound (EUS)-guided biliary drainage techniques advance, EUS-RV ERCP has been increasingly employed to guide bile duct access and cannulation with satisfactory clinical outcomes and is especially valuable for benign pathology at centres where expertise is available. Endoscopists should become familiar with each technique’s advantages and limitations before deciding the most appropriate treatment that is tailored to patient’s anatomy and clinical needs.
The “Pull Factor” Problematization in the Emergence of Everyday Bordering in the UK Welfare State
Authors: Slaven Mike
Published: October 2021

The “everyday bordering” concept has provided key insights into the effects of diverse bordering practices upon social life, placing the bordering of the welfare state among wider state interventions in an autochthonous politics of belonging. Sociological contributions have also introduced new explanations as to why states pursue such measures, positing that neoliberal states seek legitimacy through increasing activities to (re)affirm borders within this politics of belonging, compensating for a failure to govern the economy in the interests of citizens. To what extent is this visible in the state-led emergence of (everyday) borders around welfare in the United Kingdom, often cited as a key national case? This article draws from 20 elite interviews to contribute to genealogical accounts of the emergence of everyday bordering through identifying the developing “problematizations” connected to this kind of bordering activity, as the British state began to distinctly involve welfare-state actors in bordering policies in the 1990s and early 2000s. This evidence underlines how these policies were tied to a “pull factor” problematization of control failure, where the state needed to reduce various “pull factors” purportedly attracting unwanted migrants in order to control immigration per se, with little evidence that legitimacy issues tied to perceived declining economic governability informed these developments in this period. These findings can inform future genealogical analyses that trace the emergence of everyday bordering.
Decoding the Equine Genome: Lessons from ENCODE
Authors: Peng Sichong , Petersen Jessica L. , Bellone Rebecca R. , Kalbfleisch Ted , Kingsley N. B. , Barber Alexa M. , Cappelletti Eleonora , Giulotto Elena , Finno Carrie J.
Published: October 2021

The horse reference genome assemblies, EquCab2.0 and EquCab3.0, have enabled great advancements in the equine genomics field, from tools to novel discoveries. However, significant gaps of knowledge regarding genome function remain, hindering the study of complex traits in horses. In an effort to address these gaps and with inspiration from the Encyclopedia of DNA Elements (ENCODE) project, the equine Functional Annotation of Animal Genome (FAANG) initiative was proposed to bridge the gap between genome and gene expression, providing further insights into functional regulation within the horse genome. Three years after launching the initiative, the equine FAANG group has generated data from more than 400 experiments using over 50 tissues, targeting a variety of regulatory features of the equine genome. In this review, we examine how valuable lessons learned from the ENCODE project informed our decisions in the equine FAANG project. We report the current state of the equine FAANG project and discuss how FAANG can serve as a template for future expansion of functional annotation in the equine genome and be used as a reference for studies of complex traits in horse. A well-annotated reference functional atlas will also help advance equine genetics in the pan-genome and precision medicine era.
A Long Contiguous Stretch of Homozygosity Disclosed a Novel STAG3 Biallelic Pathogenic Variant Causing Primary Ovarian Insufficiency: A Case Report and Review of the Literature
Authors: Mellone Simona , Zavattaro Marco , Vurchio Denise , Ronzani Sara , Caputo Marina , Leone Ilaria , Prodam Flavia , Giordano Mara
Published: October 2021

Primary ovarian insufficiency (POI) refers to an etiologically heterogeneous disorder characterized by hypergonadotropic hypogonadism that represents a major cause of infertility in women under 40 years of age. Most cases are apparently sporadic, but about 10–15% have an affected first-degree relative, indicating a genetic etiology. Pathogenic variations in genes involved in development, meiosis and hormonal signaling have been detected in the hereditary form of the disorder. However, most cases of POI remain unsolved even after exhaustive investigation. A 19-year-old Senegalese female affected by non-syndromic POI presented with primary amenorrhoea and answered well to the hormonal induction of puberty. In order to investigate the presence of a genetic defect, aCGH-SNP analysis was performed. A 13.5 Mb long contiguous stretch of homozygosity (LCSH) was identified on chromosome 7q21.13-q22.1 where the exome sequencing revealed a novel homozygous 4-bp deletion (c.3381_3384delAGAA) in STAG3. Pathogenic variants in this gene, encoding for a meiosis-specific protein, have been previously reported as the cause of POI in only eight families and recently as the cause of infertility in a male. The here-identified mutation leads to the truncation of the last 55 amino acids, confirming the important role in meiosis of the STAG3 C-terminal domain.
Population Genetic Structures of Puccinia striiformis f. sp. tritici in the Gansu-Ningxia Region and Hubei Province, China
Authors: Wang Cuicui , Jiang Bingbing , Liang Junmin , Li Leifu , Gu Yilin , Li Jintang , Luo Yong , Ma Zhanhong
Published: October 2021

Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), is a destructive wheat disease in China. The Gansu–Ningxia region (GN) is a key area for pathogen over-summering in China, and northwestern Hubei (HB) is an important region for pathogen over-wintering, serving as a source of inoculum in spring epidemic regions. The spatiotemporal population genetic structure of Pst in HB and the pathogen population exchanges between GN and HB are important for estimating the risk of interregional epidemics. Here, 567 isolates from GN and HB were sampled from fall 2016 to spring 2018 and were genotyped using simple sequence repeat markers. The genotypic and genetic diversity of Pst subpopulations in HB varied among seasons and locations. Greater genetic diversification levels were found in the spring compared with fall populations using principal coordinate analysis and Bayesian assignments. In total, there were 17 common genotypes among the 208 determined, as shown by a small overlap of genotypes in the principal coordinate analysis and dissimilar Bayesian assignments in both regions, which revealed the limited genotype exchange between the populations of GN and HB.
A Simplified Way to Evaluate the Effect of Temperature on a Circular Tunnel
Authors: Du Dianchun , Dias Daniel , Do Ngoc-Anh
Published: October 2021

Temperature differences between the surrounding ground and the tunnel lining will cause a variation of the tunnel lining forces. The hyperstatic reaction method (HRM) could be efficiently and simply used to investigate the impact of thermal load on tunnel linings. First, the steady state numerical solution is derived for a shallow circular tunnel to estimate the internal forces and displacements of tunnel lining caused by thermal load. The effective strain coefficient βTl is deduced to calculate the thermal stresses in tunnel lining. Secondly, the influence of the temperature difference on the internal forces of tunnel lining is investigated using the HRM method considering different lining elastic modulus, lining thickness and ground coefficient of thermal expansion. Lastly, the impact of fires which will be able to modify the elastic modulus of tunnel lining is investigated, which makes it possible to predict the damage of tunnel lining caused by fires.
From Missionaries of Socialism to Spies of Imperialism: The Shifting Position of Soviet Women in Communist Albania
Authors: Hoxha Artan R.
Published: October 2021

After the establishment of the communist regime in Albania, many Albanian students, mainly males, went to study in the Mecca of Revolution—the Union of Soviet Socialist Republic (USSR). Many of them fell in love there and married Soviet girls who returned with them to the tiny Balkan country to build socialism with their Albanian husbands. These women were considered as missionaries who were helping Albania to build a communist future. In 1960, however, their position changed when the Albanian leadership refused de-Stalinization and denounced the Soviet Union as an imperialist power. After Enver Hoxha’s split with Khrushchev, many Soviet women left Albania, but others decided to remain with their husbands in that country. Albanian authorities, considering Soviet women spies of the KGB (The Soviet Committee of State Security), persecuted many of them.
The Effect of Exercise Compliance on Risk Reduction for Hamstring Strain Injury: A Systematic Review and Meta-Analyses
Authors: Ripley Nicholas Joel , Cuthbert Matthew , Ross Steven , Comfort Paul , McMahon John James
Published: October 2021

Eccentric strength training can reduce the risk of hamstring strain injury (HSI) occurrence; however, its implementation can be impacted by athlete compliance and prescription. The aim of this review was to investigate the effects of intervention compliance, consistency and modality, on the prevention of HSIs among athletes. A literature search was conducted. 868 studies were identified prior to the application of the exclusion criteria which resulted in 13 studies identified. Random effects models were used to produce log odds ratios and 95% confidence intervals. Very high (>75.1%), moderate-high (50.1–75%), low-moderate (25.1–50%) and very low (<25%) and <1-, 1.01–3.00-, >3.01-weeks/session were used as thresholds of compliance and consistency, respectively. Modality was also observed. A positive effect on HSI incidence -0.61 (−1.05 to −0.17), favoring the intervention treatments (Z = −2.70, p = 0.007). There were non-significant, large differences between compliance (p = 0.203, Z = −1.272) and consistency (p = 0.137, Z = −1.488), with increased compliance and consistency showing greater effectiveness. A significant difference between intervention modalities was observed (p < 0.001, Z = −4.136), with eccentric interventions being superiorly effective. Compliance of >50.1% and consistent application with <3 weeks/session having positive effects on HSI incidence. Training interventions that can achieve high levels of compliance, and can be consistently performed, should be the objective of future practice.
Maternal Reflective Functioning and Attachment Security in Girls and Boys: A Closer Look into the Middle Childhood
Authors: Čekuolienė Danguolė , Gervinskaitė-Paulaitienė Lina , Grauslienė Izabelė , Adler Asta , Barkauskienė Rasa
Published: October 2021

Child attachment undergoes major changes during middle childhood. Maternal reflective functioning (RF) is hypothesized to be an important correlate of a child’s attachment security during this period; however, the child’s gender role in this association has not been examined yet. In the present study, we used 64 mother-child (6–11 years old) dyads from a community sample to analyze the association between maternal RF and child attachment security and whether this link is moderated by gender. Maternal RF was assessed on the Parent Development Interview Revised (PDI-R2) and child attachment classifications were examined by the Child Attachment Interview (CAI). Results revealed the positive and statistically significant association between maternal RF and child attachment security in the whole sample. Further evaluations of this link in the groups of girls and boys separately indicated its significance for girls only. Finally, moderation analysis demonstrated the relation between child attachment security and maternal RF to be moderated by gender. These findings provide a new knowledge on gender role in attachment security in relation to maternal RF as well as suggest possible differentiation in the correlates on the pathway of secure attachment between girls and boys during middle childhood.
Neighborhood-Based Social Capital and Depressive Symptoms among Adults: Evidence from Guangzhou, China
Authors: Mao Sanqin , Chen Jie
Published: October 2021

This study examined the association between neighborhood-based social capital (NSC) and depressive symptoms in the context of urban neighborhoods in China, with special attention given to the association heterogeneity across socioeconomic groups. Drawing on cross-sectional data collected from 39 neighborhoods in Guangzhou, this research demonstrated that adults’ depressive symptoms were higher among those with lower cognitive (trustworthiness, reciprocity, and cohesion within a neighborhood) and structural (social network and participation) dimensions of NSC. Further analysis showed that the negative association between NSC and depressive symptoms was significantly heterogeneous across socioeconomic groups. Specifically, this negative relationship was more prominent in the lower socioeconomic classes than in the upper socioeconomic classes, indicating that the lower accumulation of NSC among disadvantaged groups may aggravate depression unequally across social classes. In addition, the negative association between social participation and depressive symptoms was stronger for people who are older or unemployed. The findings of this study not only provide new evidence concerning the significance of the beneficial effects of NSC in the Chinese context, but also, more importantly, highlight that NSC plays a crucial role in creating mental health inequality across social classes. Thus, the relevant social interventions including fostering neighborhood relationships and social activities should be carefully tailored against the backdrop of community building during the urbanization process. The implications of our study for urban governance to promote healthy cities are discussed.
Views of Own Body Weight and the Perceived Risks of Developing Obesity and NCDs in South African Adults
Authors: Manafe Mashudu , Chelule Paul K. , Madiba Sphiwe
Published: October 2021

Obesity has become a serious public health problem worldwide and is linked to an increased risk of non-communicable diseases (NCDs). Poor self-perception of body weight is postulated to play a negative role in curbing increased rates of obesity. This study investigated the views of own body weight and perceived risk of developing NCDs in South African adults. This was a community-based quantitative study. Descriptive statistics were used, and logistic regression analysis was conducted on the data. A total of 1050 respondents took part in the study. Of the 161 respondents who perceived themselves to be normal weight, 98.8% (n = 159) misperceived their body weight. The majority of respondents (>90%) who were overweight according to the calculated BMI perceived no risk of developing obesity-related diseases. Most of the respondents, 46% (n = 253), believed that body weight was influenced by heredity. The method used for weight loss by 57% (n = 173) of the respondents was exercising at home. There was a statistically significant association between Body Mass Index (BMI), employment status, risk of developing diabetes, and body weight misperception (p < 0.05). Misperception of body weight was common among the study respondents and may influence weight control intervention strategies. Health promotion targeting personal behaviour, such as body weight self-perception, is crucial in supporting targeted strategies to address obesity in South Africa.
Racism and Cancer Screening among Low-Income, African American Women: A Multilevel, Longitudinal Analysis of 2-1-1 Texas Callers
Authors: Ibekwe Lynn N. , Fernández-Esquer Maria Eugenia , Pruitt Sandi L. , Ranjit Nalini , Fernández Maria E.
Published: October 2021

Although racism is increasingly being studied as an important contributor to racial health disparities, its relation to cancer-related outcomes among African Americans remains unclear. The purpose of this study was to help clarify the relation between two indicators of racism—perceived racial discrimination and racial residential segregation—and cancer screening. We conducted a multilevel, longitudinal study among a medically underserved population of African Americans in Texas. We assessed discrimination using the Experiences of Discrimination Scale and segregation using the Location Quotient for Racial Residential Segregation. The outcome examined was “any cancer screening completion” (Pap test, mammography, and/or colorectal cancer screening) at follow-up (3–10 months post-baseline). We tested hypothesized relations using multilevel logistic regression. We also conducted interaction and stratified analyses to explore whether discrimination modified the relation between segregation and screening completion. We found a significant positive relation between discrimination and screening and a non-significant negative relation between segregation and screening. Preliminary evidence suggests that discrimination modifies the relation between segregation and screening. Racism has a nuanced association with cancer screening among African Americans. Perceived racial discrimination and racial residential segregation should be considered jointly, rather than independently, to better understand their influence on cancer screening behavior.
Can Social Cognitive Theory Influence Breakfast Frequency in an Institutional Context: A Qualitative Study
Authors: Harris Jessica A , Carins Julia , Rundle-Thiele Sharyn
Published: October 2021

Breakfast is considered an important meal, especially for people who are about to commence a long or demanding workday, and for roles that may involve physical tasks and a requirement to remain alert and vigilant in potentially high-risk situations. This study looks at breakfast consumption influences within two workplace institutional settings, namely military and mining. Semi-structured interviews were conducted with military personnel (n = 12) and mining employees (n = 12) to understand their breakfast consumption behaviour at work and at home, and the associated behavioural influences. The interview questions were framed by social cognitive theory. Overall, cognitive and environmental influences were the most prominent influences on breakfast consumption, less evident were behavioural influences. A negative stereotype of workplace institutional food services emerged as one of the most significant barriers to breakfast consumption for those already at work. Considerations of environmental influences on behaviour may need to be broadened beyond physical barriers and social influences, to include perceptions of the behavioural environment. Programs that aim to increase breakfast consumption must create areas where their employees want to go. Food systems need to ensure nutritious, quality, and appealing food is available. Interventions need to increase participants’ knowledge, improve their attitudes, and create positive expectations for breakfast.
Association between Self-Reported Exposure to Alcohol Advertisements and Drinking Behaviors: An Analysis of a Population-Based Survey in Thailand
Authors: Boontem Phagapun , Saengow Udomsak
Published: October 2021

The relationship between alcohol advertising and drinking has been demonstrated in many studies. Most studies were conducted on adolescents or young adults. Thailand has strict regulations on alcohol advertisements. This study aimed to examine associations between exposure to alcohol advertisements and drinking behaviors, i.e., past-year drinking and past-year heavy drinking, using data from a population-based survey in Thailand. The survey participants were Thai citizens aged 15 or older. Logistic regression was used to investigate the associations. The primary explanatory variable was self-reported exposure to alcohol advertisements. Covariates in the regression models included sex, age, and education. Self-reported exposure to alcohol advertisements was associated with past-year drinking (OR, 1.16; 95% CI, 1.07–1.27), past-year heavy drinking (OR, 1.35; 95% CI, 1.28–1.41), and past-year heavy drinking among drinkers (OR, 1.51; 95% CI, 1.43–1.60). Male sex, working age, and secondary education or a diploma were associated with higher odds of past-year drinking and past-year heavy drinking. In this study, self-reported exposure to alcohol advertisements was shown to be associated with past-year drinking and past-year heavy drinking among a population aged 15 years or older.
Short-Term Aerobic Exercise Did Not Change Telomere Length While It Reduced Testosterone Levels and Obesity Indexes in PCOS: A Randomized Controlled Clinical Trial Study
Authors: Ribeiro Victor Barbosa , Pedroso Daiana Cristina Chielli , Kogure Gislaine Satyko , Lopes Iris Palma , Santana Barbara Aparecida , Dutra de Souza Hugo Celso , Ferriani Rui Alberto , Calado Rodrigo Tocantins , Furtado Cristiana Libardi Miranda , Reis Rosana Maria dos
Published: October 2021

Metabolic and hormonal outcomes of polycystic ovary syndrome (PCOS) have implications on telomere biology and physical activity may prevent telomere erosion. We sought to observe the effects of continuous (CAT) and intermittent (IAT) aerobic training on telomere length, inflammatory biomarkers, and its correlation with metabolic, hormonal, and anthropometric parameters of PCOS. This randomized controlled clinical trial study included 87 PCOS randomly stratified according to body mass index (BMI) in CAT (n = 28), IAT (n = 29) and non-training control group (CG, n = 30). The exercises were carried out on a treadmill, three times per week for 16 weeks. The participants’ anthropometric characteristics and biochemical and hormonal concentrations were measured before and after aerobic training or observation period, as the telomere length that was evaluated using quantitative real-time PCR. Four months of aerobic exercises (CAT or IAT) did not alter telomere length and inflammatory biomarkers in PCOS women. Obesity index as BMI and waist circumference (WC), and inflammatory biomarkers negatively affect telomeres. The hyper-andro-genism measured by testosterone levels was reduced after both exercises (CAT, p ≤ 0.001; IAT, p = 0.019). In particular, the CAT reduced WC (p = 0.045), hip circumference (p = 0.032), serum cholesterol (p ≤ 0.001), and low-density lipoprotein (p = 0.030). Whereas, the IAT decreased WC (p = 0.014), waist-to-hip ratio (p = 0.012), free androgen index (FAI) (p = 0.037). WC (p = 0.049) and body fat (p = 0.015) increased in the non-training group while total cholesterol was reduced (p = 0.010). Booth exercises reduced obesity indices and hyperandrogenism on PCOS women without changes in telomere length or inflammatory biomarkers.
Association between Lifetime Tobacco Use and Alcohol Consumption Trajectories and Cardiovascular and Chronic Respiratory Diseases among Older People
Authors: Madero-Cabib Ignacio , Bambs Claudia
Published: October 2021

Background: We identify representative types of simultaneous tobacco use and alcohol consumption trajectories across the life course and estimate their association with cardiovascular and chronic respiratory diseases (CVDs and CRDs) among older people in Chile. Methods: We used data from a population-representative, face-to-face and longitudinal-retrospective survey focused on people aged 65–75 (N = 802). To reconstruct trajectory types, we employed weighted multichannel sequence analysis. Then, we estimated their associations with CVDs and CRDs through weighted logistic regression models. Results: Long-term exposure to tobacco use and alcohol consumption across life are associated with the highest CVD and CRD risks. Long-term nonsmokers and nondrinkers do not necessarily show the lowest CVDs and CRDs risks if these patterns are accompanied by health risk factors such as obesity or social disadvantages such as lower educational levels. Additionally, trajectories showing regular consumption in one domain but only in specific periods of life, whether early or late, while maintaining little or no consumption across life in the other domain, lead to lower CVDs or CRDs risks than trajectories indicating permanent consumption in both domains. Conclusions: A policy approach that considers CVDs and CRDs as conditions that strongly depend on previous individual experiences in diverse life domains can contribute to the improved design and evaluation of preventive strategies of tobacco use and alcohol consumption across the life course.
Assessment of Quality of Life and Pain Severity in Older Men with Osteoporosis: Cross-Sectional Study
Authors: Nawrat-Szołtysik Agnieszka , Miodońska Zuzanna , Piejko Laura , Szołtys Bogna , Błaszczyszyn Monika , Matyja Beata , Zarzeczny Ryszard , Zając-Gawlak Izabela , Kucio Ewa , Polak Anna
Published: October 2021

Background: The quality of life in osteoporosis is studied for men rather than for women. Aim of the study was to determine how bone mass density (BMD) relates to life quality components and the severity of pain felt by men affected by osteoporosis. Methods: Presented research is a cross-sectional study. The cohort of 62 men aged 65 to 85 years was divided into a group with osteoporosis (N = 27) and a group without osteoporosis (N = 35). The participants’ quality of life was measured with the Qualeffo41 Questionnaire, BMD was quantified by densitometry, and pain intensity was assessed on the Visual Analogue Scale. Results: We found that lower BMD was strongly correlated to participants’ quality of life (r = −0.72), especially the quality of leisure and social activities (r = −0.66), general health perception (r = −0.59), and mobility (r = −0.57). Pain significantly affected general health perception in older men with osteoporosis. General health assessment and pain were highly correlated with each other (r = 0.888). Conclusion: BMD and the overall quality of life of the study participants were related to each other. The strongest relationship occurred between reduced BMD and leisure and social activities component. The pain significantly affected participants’ general health perception. The results may be employed to create new prophylactic strategies to improve life quality in men with osteoporosis.
Correction: Lee et al. Patterns of Multimorbidity in Adults: An Association Rules Analysis Using the Korea Health Panel. Int. J. Environ. Res. Public Health 2020, 17, 2618
Authors: Lee Yoonju , Kim Heejin , Jeong Hyesun , Noh Yunhwan
Published: October 2021

The authors have noticed an inadvertent error in our article, ‘‘Patterns of Multimorbidity in Adults: An Association Rules Analysis Using the Korea Health Panel” [...]
Age-Related Digital Divide during the COVID-19 Pandemic in China
Authors: Song Yu , Qian Chenfei , Pickard Susan
Published: October 2021

China has adopted a variety of digital technologies to effectively combat the unprecedented COVID-19 pandemic. The massive utilisation of digital technologies, however, to a great extent, magnifies the age-related digital divide. This paper aims to examine the impacts of the age-related digital divide on older adults in the context of the COVID-19 pandemic. Cases of three age-related digital divide scenarios, including older people taking public transportation, seeking medical care, as well as conducting digital transactions, are collected from Chinese official news outlets. The results indicate that the COVID-19 pandemic accelerates the pace of digital technology utilisation but exacerbates the age-related digital divide. Such an age-related digital divide has largely excluded older adults from both the real society and the virtual society. Older adults’ personal attitudes and motivations, as well as education and income, governmental policies, and family and social supports, are all major contributors to the severe impacts of the age-related digital divide on old adults during the pandemic. More measures should be adopted to bridge the age-related digital divide and build a senior-friendly e-society.
Reduction in Absolute Neutrophil Counts in Patient on Clozapine Infected with COVID-19
Authors: Ramli Fitri Fareez , Ali Adli , Syed Hashim Syed Alhafiz , Kamisah Yusof , Ibrahim Normala
Published: October 2021

Despite its severe adverse effects, such as agranulocytosis, clozapine is the primary treatment for treatment-resistant schizophrenia. The established clozapine monitoring system has contributed to reducing agranulocytosis incidence and mortality rates. However, the pandemic coronavirus disease 2019 (COVID-19) has caused changes in the monitoring system. This review aimed to assess the current evidence on the neutrophil changes in the patient on clozapine treatment and infected with COVID-19. Individual cases reported various absolute neutrophil count (ANC) levels, normal, reduced, or elevated. No agranulocytosis case was reported. One case had a borderline moderate-severe ANC level, but the patient was in the 18-week period of clozapine treatment. A cumulative analysis of case the series initially reported inconclusive results. However, a more recent study with a larger sample size reported a significant reduction in the ANC during COVID-19 infection. Nevertheless, this effect is transient as no significant difference was found between the baseline and the post-infection period in ANC levels. In conclusion, COVID-19 is associated with a temporary reduction in ANC levels. The results supported the recommendation to reduce the frequency of clozapine monitoring in the eligible candidates. However, more data are required to confirm the current findings given the limitations, including study design, sample size, and statistical analysis.
Italian Expert Consensus on Clinical and Therapeutic Management of Multiple Chemical Sensitivity (MCS)
Authors: Damiani Giovanni , Alessandrini Marco , Caccamo Daniela , Cormano Andrea , Guzzi Gianpaolo , Mazzatenta Andrea , Micarelli Alessandro , Migliore Alberto , Piroli Alba , Bianca Margherita , Tapparo Ottaviano , Pigatto Paolo
Published: October 2021

Multiple chemical sensitivity (MCS) is a multisystem, recurrent, environmental disorder that flares in response to different exposures (i.e., pesticides, solvents, toxic metals and molds) under the threshold limit value (TLV) calculated for age and gender in the general population. MCS is a syndrome characterized by cutaneous, allergic, gastrointestinal, rheumatological, endocrinological, cardiological and neurological signs and symptoms. We performed a systematic review of the literature to summarize the current clinical and therapeutic evidence and then oriented an eDelphi consensus. Four main research domains were identified (diagnosis, treatment, hospitalization and emergency) and discussed by 10 experts and an MCS patient. Thus, the first Italian MCS consensus had the double aim: (a) to improve MCS knowledge among healthcare workers and patients by standardizing the clinical and therapeutic management to MCS patients; and (b) to improve and shed light on MCS misconceptions not supported by evidence-based medicine (EBM).
Knowledge, Attitude, and Practice of Evidence-Based Medicine among Emergency Doctors in Kelantan, Malaysia
Authors: Ahmad Ghaus Mohd Ghouse , Tuan Kamauzaman Tuan Hairulnizam , Norhayati Mohd Noor
Published: October 2021

This study aimed to determine the prevalence of high levels of knowledge, positive attitude, and good practice on evidence-based medicine (EBM) and identify the associated factors for practice score on EBM among emergency medicine doctors in Kelantan, Malaysia. This cross-sectional study was conducted in government hospitals in Kelantan. The data were collected from 200 emergency physicians and medical officers in the emergency department using the Noor Evidence-Based Medicine Questionnaire. Simple and general linear regressions analyses using SPSS were performed. A total of 183 responded, making a response rate of 91.5%. Of them, 49.7% had a high level of knowledge, 39.9% had a positive attitude and 2.1% had good practice. Sex, race, the average number of patients seen per day, internet access in workplace, having online quick reference application, and attitude towards EBM were significantly associated with EBM practice scores. It is recommended that appropriate authorities provide emergency doctors with broader access to evidence resources. EBM skill training should be enhanced in the current medical school curriculums.
Enhancing the Visibility of SuDS in Strategic Planning Using Preliminary Regional Opportunity Screening
Authors: Webber James L. , Kuller Martijn
Published: October 2021

Surface water flooding poses significant threats to communities and environments. This threat has historically been managed through sewers and combined sewer overflows; however, it is now well recognised that, alone, these legacy systems are insufficient to manage the growing pressures from climate change, population growth and urbanisation. This realisation has led to research and practice developing a broad range of new technologies to enhance the coping capacities of existing sewer systems through capturing and attenuating or reusing surface water across catchments using sustainable drainage systems (SuDS). However, despite technical understanding, industry champions and significant best practice, SuDS remain underutilised, particularly at a synergistic catchment scale where they can be most effective. In this paper we respond to this challenge by developing preliminary screening tools to enhance the visibility of SuDS among the multidisciplinary decision-makers responsible for directing strategic surface water management. We achieve this through upscaling a regional decision support model and demonstrating implementation across a case study in South West England. We find that it is possible to use easily accessible and open-source data to provide initial indications of SuDS opportunities and that this early visibility in the decision-making process can be used to support the consideration of novel and effective surface water management strategies.
QRB-tree Indexing: Optimized Spatial Index Expanding upon the QR-tree Index
Authors: Yu Jieqing , Wei Yi , Chu Qi , Wu Lixin
Published: October 2021

Support for region queries is crucial in geographic information systems, which process exact queries through spatial indexing to filter features and subsequently refine the selection. Although the filtering step has been extensively studied, the refinement step has received little attention. This research builds upon the QR-tree index, which decomposes space into hierarchical grids, registers features to the grids, and builds an R-tree for each grid, to develop a new QRB-tree index with two levels of optimization. In the first level, a bucket is introduced in every grid in the QR-tree index to accelerate the loading and search steps of a query region for the grids within the query region. In the second level, the number of candidate features to be eliminated is reduced by limiting the features to those registered to the grids covering the corners of the query region. Subsequently, an approach for determining the maximal grid level, which significantly affects the performance of the QR-tree index, is proposed. Direct comparisons of time costs with the QR-tree index and geohash index show that the QRB-tree index outperforms the other two approaches for rough queries in large query regions and exact queries in all cases.
TAAR1 Expression in Human Macrophages and Brain Tissue: A Potential Novel Facet of MS Neuroinflammation
Authors: Barnes David A. , Galloway Dylan A. , Hoener Marius C. , Berry Mark D. , Moore Craig S.
Published: October 2021

TAAR1 is a neuroregulator with emerging evidence suggesting a role in immunomodulation. Multiple sclerosis (MS) is an immune-mediated demyelinating disease of the central nervous system. Here, we investigate TAAR1 expression in human primary monocytes, peripherally-derived macrophages, and MS brain tissue. RT-qPCR was used to assess TAAR1 levels in MS monocytes. Using a previously validated anti-human TAAR1 antibody and fluorescence microscopy, TAAR1 protein was visualized in lipopolysaccharide-stimulated or basal human macrophages, as well as macrophage/microglia populations surrounding, bordering, and within a mixed active/inactive MS lesion. In vivo, TAAR1 mRNA expression was significantly lower in MS monocytes compared to age- and sex-matched healthy controls. In vitro, TAAR1 protein showed a predominant nuclear localization in quiescent/control macrophages with a shift to a diffuse intracellular distribution following lipopolysaccharide-induced activation. In brain tissue, TAAR1 protein was predominantly expressed in macrophages/microglia within the border region of mixed active/inactive MS lesions. Considering that TAAR1-mediated anti-inflammatory effects have been previously reported, decreased mRNA in MS patients suggests possible pathophysiologic relevance. A shift in TAAR1 localization following pro-inflammatory activation suggests its function is altered in pro-inflammatory states, while TAAR1-expressing macrophages/microglia bordering an MS lesion supports TAAR1 as a novel pharmacological target in cells directly implicated in MS neuroinflammation.
Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of Aspergillus nidulans
Authors: Bákány Bernadett , Yin Wen-Bing , Dienes Beatrix , Nagy Tibor , Leiter Éva , Emri Tamás , Keller Nancy P. , Pócsi István
Published: October 2021

Basic leucine zipper (bZIP) transcription factors play a crucial role in the environmental stress response of eukaryotes. In this work, we studied the effect of gene manipulations, including both deletions and overexpressions, of two selected bZIP transcription factors, NapA and RsmA, in the oxidative stress response and sterigmatocystin production of Aspergillus nidulans. We found that NapA was important in the oxidative stress response by negatively regulating intracellular reactive species production and positively regulating catalase activities, whereas RsmA slightly negatively regulated catalase activities. Concerning sterigmatocystin production, the highest concentration was measured in the ΔrsmAΔnapA double deletion mutant, but elevated sterigmatocystin production was also found in the OErsmA OEnapA strain. Our results indicate that NapA influences sterigmatocystin production via regulating reactive species level whereas RsmA modulates toxin production independently of the redox regulation of the cells.
Novel Biomolecules in the Pathogenesis of Gestational Diabetes Mellitus
Authors: Ruszała Monika , Niebrzydowska Magdalena , Pilszyk Aleksandra , Kimber-Trojnar Żaneta , Trojnar Marcin , Leszczyńska-Gorzelak Bożena
Published: October 2021

Gestational diabetes mellitus (GDM) is one of the most common metabolic diseases in pregnant women. Its early diagnosis seems to have a significant impact on the developing fetus, the course of delivery, and the neonatal period. It may also affect the later stages of child development and subsequent complications in the mother. Therefore, the crux of the matter is to find a biopredictor capable of singling out women at risk of developing GDM as early as the very start of pregnancy. Apart from the well-known molecules with a proven and clear-cut role in the pathogenesis of GDM, e.g., adiponectin and leptin, a potential role of newer biomolecules is also emphasized. Less popular and less known factors with different mechanisms of action include: galectins, growth differentiation factor-15, chemerin, omentin-1, osteocalcin, resistin, visfatin, vaspin, irisin, apelin, fatty acid-binding protein 4 (FABP4), fibroblast growth factor 21, and lipocalin-2. The aim of this review is to present the potential and significance of these 13 less known biomolecules in the pathogenesis of GDM. It seems that high levels of FABP4, low levels of irisin, and high levels of under-carboxylated osteocalcin in the serum of pregnant women can be used as predictive markers in the diagnosis of GDM. Hopefully, future clinical trials will be able to determine which biomolecules have the most potential to predict GDM.
Molecular Mechanisms of Muscle Fatigue
Authors: Constantin-Teodosiu Dumitru , Constantin Despina
Published: October 2021

Muscle fatigue (MF) declines the capacity of muscles to complete a task over time at a constant load. MF is usually short-lasting, reversible, and is experienced as a feeling of tiredness or lack of energy. The leading causes of short-lasting fatigue are related to overtraining, undertraining/deconditioning, or physical injury. Conversely, MF can be persistent and more serious when associated with pathological states or following chronic exposure to certain medication or toxic composites. In conjunction with chronic fatigue, the muscle feels floppy, and the force generated by muscles is always low, causing the individual to feel frail constantly. The leading cause underpinning the development of chronic fatigue is related to muscle wasting mediated by aging, immobilization, insulin resistance (through high-fat dietary intake or pharmacologically mediated Peroxisome Proliferator-Activated Receptor (PPAR) agonism), diseases associated with systemic inflammation (arthritis, sepsis, infections, trauma, cardiovascular and respiratory disorders (heart failure, chronic obstructive pulmonary disease (COPD))), chronic kidney failure, muscle dystrophies, muscle myopathies, multiple sclerosis, and, more recently, coronavirus disease 2019 (COVID-19). The primary outcome of displaying chronic muscle fatigue is a poor quality of life. This type of fatigue represents a significant daily challenge for those affected and for the national health authorities through the financial burden attached to patient support. Although the origin of chronic fatigue is multifactorial, the MF in illness conditions is intrinsically linked to the occurrence of muscle loss. The sequence of events leading to chronic fatigue can be schematically denoted as: trigger (genetic or pathological) -> molecular outcome within the muscle cell -> muscle wasting -> loss of muscle function -> occurrence of chronic muscle fatigue. The present review will only highlight and discuss current knowledge on the molecular mechanisms that contribute to the upregulation of muscle wasting, thereby helping us understand how we could prevent or treat this debilitating condition.
VDAC1 Negatively Regulates Floral Transition in Arabidopsis thaliana
Authors: Xu Jingya , Zhang Yuzhen , Ren Hongjia , Yu Runyi , Yuan Chen , Hu Yikai , Xu Rumeng , Wang Xuming , Qin Cheng
Published: October 2021

Voltage-dependent anion channels (VDACs) are the most important proteins in mitochondria. They localize to the outer mitochondrial membrane and contribute to the metabolite transport between the mitochondria and cytoplasm, which aids plant growth regulation. Here, we report that Arabidopsis thaliana VDAC1 is involved in the floral transition, with the loss of AtVDAC1 function, resulting in an early-flowering phenotype. AtVDAC1 is expressed ubiquitously in Arabidopsis. To identify the flowering pathway integrators that may be responsible for AtVDAC1′s function during the floral transition, an RNA-seq analysis was performed. In total, 106 differentially expressed genes (DEGs) were identified between wild-type and atvdac1-5 mutant seedlings. However, none were involved in flowering-related pathways. In contrast, AtVDAC1 physically associated with FLOWERING LOCUS T. Thus, in the floral transition, AtVDAC1 may function partly through the FLOWERING LOCUS T protein.
Chronic Exposure to Paraquat Induces Alpha-Synuclein Pathogenic Modifications in Drosophila
Authors: Arsac Jean-Noël , Sedru Marianne , Dartiguelongue Mireille , Vulin Johann , Davoust Nathalie , Baron Thierry , Mollereau Bertrand
Published: October 2021

Parkinson’s disease (PD) is characterized by the progressive accumulation of neuronal intracellular aggregates largely composed of alpha-Synuclein (αSyn) protein. The process of αSyn aggregation is induced during aging and enhanced by environmental stresses, such as the exposure to pesticides. Paraquat (PQ) is an herbicide which has been widely used in agriculture and associated with PD. PQ is known to cause an increased oxidative stress in exposed individuals but the consequences of such stress on αSyn conformation remains poorly understood. To study αSyn pathogenic modifications in response to PQ, we exposed Drosophila expressing human αSyn to a chronic PQ protocol. We first showed that PQ exposure and αSyn expression synergistically induced fly mortality. The exposure to PQ was also associated with increased levels of total and phosphorylated forms of αSyn in the Drosophila brain. Interestingly, PQ increased the detection of soluble αSyn in highly denaturating buffer but did not increase αSyn resistance to proteinase K digestion. These results suggest that PQ induces the accumulation of toxic soluble and misfolded forms of αSyn but that these toxic forms do not form fibrils or aggregates that are detected by the proteinase K assay. Collectively, our results demonstrate that Drosophila can be used to study the effect of PQ or other environmental neurotoxins on αSyn driven pathology.