The effect of inositol on the content of oxidative modified proteins in the liver and heart of different sexes rats with metabolic associated steatotic liver disease
Authors: Sopel О.V. , Levchuk О.О. , Denefil O.V. 
Published: August 2024

Background. The problem of metabolic disorders and, in particular, metabolically associated steatotic hepatic disease (MASHD) is one of the most relevant today. The disease is complicated by many metabolic diseases, including obesity, type 2 diabetes, and hypertension. There are also changes in the heart, kidneys, and nervous system. Such complications are associated and considered as a hepatic manifestation of metabolic syndrome. Since the integrity of the cells is violated, it is advisable to follow the mechanisms that cause them. Among them is the development of oxidative stress, which includes damage to lipids and proteins. Inositols are used to treat steatotic liver disease associated with metabolic dysfunction.

Purpose – to determine features of changes in the content of oxidative modified proteins in homogenates of the liver and heart of different sexes rats with experimentally simulated metabolic associated steatotic liver disease and to investigate the influence of inositol isoforms on their content.

Materials and Methods. Experiments were performed on 216 rats of different sexes. The animals were divided into 9 groups – control, myo-inositol, D-chiro-inositol, MASHD 2 months, MASHD 2 months + myo-inositol, MASHD 2 months + D-chiroinositol, MASHD 4 months, MASHD 4 months + myo-inositol, MASHD 4 months + +D-chiro-inositol. In the MASHD group, 2 animals consumed a 20% fructose solution instead of water for 2 months. And then for 2 months they were intragastrically injected with myo-inositol (400 mg/kg) or D-chiro-inositol (30 mg/kg). In the MASHD group, 4 animals consumed a 20% fructose solution instead of water for 4 months. 2 months after the start of the experiment, they were intragastrically injected with myo-inositol (400 mg/kg) or D-chiro-inositol (30 mg/kg). The liver was taken for morphological examination, the content of oxidatively modified proteins (ОMP370, ОМP430) was determined in the homogenate of the liver and heart.

Results. The content of OMP increased in the liver and heart of rats that consumed fructose instead of water for 2 and 4 months. Changes in their content in the liver of male and female rats increased more with 4-month fructose consumption. OMP370 increased in the heart of males after longer drinking of fructose, and OMP430 in females. Inositols cause an increase in OMP, so it is not advisable to use them in healthy animals. In MASHD, inositols reduce the harmful effects of long-term fructose consumption on the liver and heart of rats, and the effect was more pronounced in animals that continued to receive fructose. Only with long-term use of fructose, D-chiro-inositol, compared to myo-inositol, had a more pronounced effect on reducing OMP370.

Conclusions. In the liver and heart of rats that consumed 20% fructose solution for 2 and 4 months, the content of oxidative modified proteins increases, which is more pronounced with longer consumption only in the liver of animals of both sexes. In the heart of males, when drinking fructose for a long time, OMP370 increases, and in females – OMP430. In metabolic associated steatotic liver disease, inositols reduce the harmful effects of long-term fructose consumption on the liver and heart of rats, and the effect was more pronounced in animals that continued to receive fructose.