bluuebunny/crossref_metadata_2025 huggingface dataset:
Sinensetin-Rich Fraction Solid Dispersion Inhibits Cancer Cell Cycle
Authors: Arifianti Lusiana , Sukardiman Sukardiman , Santosa Mulja Hadi
Published: March 2018

Drug development efforts to find new selective and safe drugs for cancer from natural resources are promising ones. The natural products are obtained in the multiple or single compounds. One of them is a sinensetin found in ethanol extract of Orthosiphon stamineus Benth. Sinensetin could inhibit cancer cell proliferation. However, it has a poor solubility so the absorption is low and then it impacts on the low bioavailability. The solubility problem in conventional drug could be solved by pharmaceutical manipulation. In the previous research, the manipulation was tried although there was no single compound found in the material tested. We found an optimal formula of a manipulation using solid disperse system of polyethylene glycol (PEG 6000) 15 times higher than sinensetin weight. This research was focused on observing the effect of the optimal formula of solid disperse system to inhibit cancer cell cycle. The cell lines used were T47D cells. The method used was flow cytometry. The result showed that the optimum formula has a consistent effect on the concentration of 40 and 60µg/mL. The sinensetin increase cell accumulation on S phase at the percentage of 18.80% (40µg/mL) and 22.21% (60µg/mL) compared to T47D normal cells. It reflects the S phase as the longest time experienced by the cells. Inhibition on S phase (S arrest) resulted from a DNA elongation. It causes an inhibition of DNA synthesis process. It could be concluded that the solid disperse of sinensetin was active to inhibit cancer cells proliferation on phase S.  Keywords: cell cycle; sinensetin; solid disperse; poor solubility
Blunted Expression of PPARa in Mice with FABP-4 and -5 Deficiency under Acute Cold Exposure
Authors: Syamsunarno Mas Rizky A.A , Putri Mirasari , Iso Tatsuya , Widyastuti Rini , Panigoro Ramdan , Kurabayashi Masahiko
Published: March 2018

Brown Adipose Tissue (BAT) is a nonshivering thermogenesis organ during cold exposure. Peroxisomal proliferator activated receptor alpha (PPARa) is the member of the nuclear hormone receptor superfamily and primarily expressed in BAT and liver. PPARa is coordinated with uncoupling protein 1 (UCP1) to regulate fatty acid metabolism in BAT. Fatty acid binding protein (FABP)-4 and-5 play role in adaptive response under fasting and cold exposure. The purpose of this study was to investigate the expression of PPARa in mice with FABP4/5 deficiency (DKO). Wildtype (WT) and DKO mice were exposed to cold for 2 hours under fed or 20 hours-fasted conditions. BAT was collected and further mRNA level of PPARa was examined using quantitative real-time PCR. As the result, PPARa gene expression in WT mice were increased 50% and 100% in fed and fasted condition respectively after cold exposure. There was no alteration in PPARa expression in  BAT of DKO mice. As conclusion, The functional FABP-4 and -5 are necessary to modulate PPARa gene expression in Brown Adipose Tissue under acute cold exposure  Keywords: Acute cold exposure; FABP4; FABP5; Fasting  PPARa
Increased Iron in Pediatric β-Thalassaemia Major Associates with CD3+, Not γδ Lymphocytes
Authors: Ghozali Mohammad , Panjaitan Ulrike , Cahyadi Adi Imam , Ghrahani Reni , Reniarti Lelani , Syamsunarno MRAA. , Panigoro Ramdan
Published: March 2018

Iron in β-thalassaemia major can act as a double-edged sword. Inefficient erythropoiesis and repeated blood transfusion therapy undertaken by these patients contribute to accumulation of iron, where at the cellular level may cause danger since the rising rate of iron in its free form was very toxic to the cells and tissues. Severe infectious disease is the 2nd major cause of death in children with this genetic inheritance disease. Such a complication can be happened when impaired immune cells, burdened with siderophilic bacterial infection which still prevalence in Indonesia, can make β-thalassaemia major susceptible to the pathogen invasion. The γδ T cells and their Vδ2+ subset functioned as innate and adaptive immune also specifically recognizes pathogen. The purpose of this study was to characterize γδ T cells and Vδ2+ subset also investigate the correlation between iron level and percentage of lymphocyte, CD3+ T cells, γδ T cells, and Vδ2+ subset then expression of T-cell receptors, they are CD3 and γδ in pediatric β-thalassaemia major. Flow cytometry was used to characterized and measure the cells parameters. Cross sectional study was done involving 51 pediatric β-Thalassaemia major patients who visit thalassemia clinic. Respectively, there was significant positive correlation between increased serum iron and ferritin level and number of lymphocyte (r = 0.15, P = 0.005) also between number of CD3+ T cells subset and ferritin level (r = 0.42, P = 0.002). In conclusion, iron overload is related to alteration of lymphocyte population and CD3+ T cells subset in pediatric β-thalassaemia major patients.Keywords: Iron, lymphocyte, γδ T cell, Vδ2+ T cell, thalassemia
Low Serum Cholesterol in Mice Pre-treated with Imperata cylindrica L. after Acute Olive Oil Gavage
Authors: Anggraeni Neni , Syamsunarno Mas Rizky A.A , Mukarromah Ghina Rahmadianti , Zada Almira , Triatin Rima Destya , Pamela Yunisa , Dhianawaty Diah
Published: March 2018

Imperata cylindrica L. extract was studied for its effect on cholesterol absorption rate in mice (Mus muscularis). Male mice (20-25g) were divided control and pre-treated group. Pretreated group was further divided into two groups that received different dose of Imperata cylindrica L. extract gavage (90mg/KgBW and 115mg/KgBW) for 2 weeks. Mice were fasted 12 hour before acutely given olive oil gavage with dosage of 10 µl/g, and blood was collected at 0, 2, and 6 hour-time points on the indicated day. Blood was collected from the retroorbital plexus at indicated time points. Total cholesterol level was significantly (p<0.05) decreased in both pretreated groups at 6 hours after acute olive oil gavage. In conclusion, there is a significant effect of Imperata cylindrica L. extract to reduce cholesterol level in serum, suggesting its potential effect as an antihypercholesterolemia therapy.   Key words: Imperata cylindrical L.; cholesterol absorption; antihypercholesterolemia; lipid absorption; herbal medicine
The Role Of IL-6 In TMPD-Treated Lupus Arthritis Mice
Authors: Indriyanti Niken
Published: March 2018

Lupus or systemic lupus erythematosus is a chronic autoimmune disease with systemic inflammation manifestations mainly in targeted organs. The appropriate animal model for lupus is necessary. The induction method by using 2,6,10,14 tetramethylpentadecane (TMPD) reveals more complex manifestations than other hydrocarbons. However, the autophagy of macrophages as an effect of TMPD makes differences to make the decision in lupus biomarker as a targeted therapy in lupus arthritis. Thus, this research focused on the role of CD68+IL-6 produced by macrophages and total IL-6 in lupus in correlation to the arthritis severity. The naïve and TMPD-treated groups (n=3) were induced by means of 0.5 ml TMPD i.p. After 6 months, the mice were sacrificed then the fresh spleens were prepared as isolated cells to be measured by using flow cytometry method. The knee joints were prepared for histology observation. The statistical analysis was performed by using T-test SPSS 22 version. The results showed the relative percentage of CD68+IL-6+ in the TMPD-treated group increased significantly (P<0.05) with the value of 62.38±9.97 %, compared to naïve group 49.70±2.34 %. Moreover, the total IL-6 did not increase significantly (P>0.05). Meanwhile, the arthritis severity score of the TMPD-treated group revealed severe erosion with the grade of 3.7±1.06, higher significantly (P<0.05) than the naïve group (0.5±0.71). The joint spaces in both groups were not significantly different. Finally, the observations gave the clear information that despite the autophagy potency, the CD68+IL-6 and the arthritis severity score were good markers in lupus preclinical study. Keywords: CD68+IL-6; inflammation; lupus arthritis; TMPD
Immunopathological Approach for Avian Influenza Virus Detection in Brain of Laying Bird with Clinical Signs of Torticollis and Curled Toe Paralysis
Authors: Yunita Niken , Wulan Ocie Harum , Wuryastuty Hastari , Wasito Raden
Published: March 2018

Infection with avian influenza virus (AIV) in laying birds field cases often lead to clinical signs of torticollis and curled toe paralysis. Clinical signs of torticollis and curled toe paralysis is similar to the clinical signs of Newcastle disease virus infection in poultry, making it difficult for the confirmation of diagnosis of infection with both viruses. Immunopathological immunohistochemistry streptavidin-biotin (IHC SB) is an antibody-based test to detect the presence of pathogens, especially AIV in poultry with the principle of antigen detection in tissue specimens. This research aims to detect AIV infection in laying birds field case with clinical signs of torticollis and curled toe paralysis. The samples (brains from 20 layer chicken) were taken from the cases of the disease in poultry in several commercial poultry farms. The chickens showed clinical signs of torticollis and curled toe paralysis, and oedema in the brain suspected of being infected AIV. After being necropsied, then the brains were tested IHC SB and observed with a digital microscope camera system and analyzed descriptively qualitative. The results showed that the avian influenza virus antigens was found in the parenchyma, neural and endothelial cells of brain blood vessels. Based on these results, we can conclude that Immunopathologic immunohistochemical staining of streptavidin-biotin can be applied for confirmation of the diagnosis of avian influenza virus (AIV) on the brain of commercial laying chickens showing torticollis and curled toe paralysis. Keywords : AIV; IHC SB; brain; torticollis; curled toe paralysis
The Efficacy of Permot (Passiflora Foetida Linn.) Leaves Crude Extract Ointment on the Healing of Skin of Rabbit with Scabies
Authors: Hastutiek Poedji , Eliyani Hana
Published: March 2018

This research aimed to know the efficacy of active compound of  Permot (P. foetida) leaves crude extract ointment as a bioacaricide in rabbit through observation of after treatment histopathological changes on skin of body part naturally infested with Sarcoptes scabiei. Permot  leaves were isolated and extracted in n-hexane solvent. Sixteen rabbits were randomly  divided into four groups, control (P0), P1, P2 and P3, the group of rabbits with scabies treated respectively with 5, 10 and 15 % of permot leaves crude extract ointment. Therapy was conducted topically on day 0; 1; 2; 3; 4; 5. Efficacy was examined from Haematoxylin Eosin (HE) stained skin histopathological features under microscope with  magnification of 100x, 400x and 1000x. The obtained skin histopatological feature data were assessed qualitatively. The application of 5 % ointment (P1) showed erosion and severe inflammatory cells infiltration in the epidermis; follicle proliferation and sebaceous were not present. In the dermis, severe sebaceous adenitis and folliculitis were evident. Massive inflammatory cells infiltration was found in hypodermis. The therapy using 5% ointment resulted 45 % success rate. The application of 10 % permot leaves crude extract ointment (P2) showed severe hypergranulation in the epidermis, severe proliferation of follicle and sebaceous gland, mild sebaceous adenitis and mild folliculitis in the dermis, as well as  moderate inflammatory cells infiltration in the hypodermis. The therapy using 10,% ointment resulted 60 % success rate. The application of 15 % ointment (P3) showed mild hypergranulation in the epidermis, mild follicle proliferation and inflammatory cells (PMN) infiltrationin the dermis; and moderate inflammatory cells infiltration in the hypodermis. The therapy using 15 % ointment resulted 90 % success rate. Keywords: scabiosis, rabbits, Passiflora foetida, ointment, histopathology
The Effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the Liver Structure of Iron overload of Rat (Rattus norvegicus)
Authors: Safitri Ratu , Reniarti Lelani , Madihah Madihah , Delia Lila , Syamsunarno Mas Rizky A.A , Panigoro Ramdan
Published: March 2018

This research was carried out to detect the effect of Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment on the liver structure of iron overload rat (Rattus norvegicus).The method of experimental used Completely Random Design (CRD in triple repetition. The treatment had been carried out orally. Iron dextran with total dose of 1.5 g kg-1 of body were given to rat on the first, fourth, seventh, ten and thirteenth day. Sappan Wood Extract (Caesalpinia sappan)200 mg kg-1 bw, 400 mg kg-1 bw, Wheat grass extract 100 mg kg-1 bw and Vitamin E 60 mg kg-1 bw were given to rat everyday for 15 days. At the seventeenth, rat were killed and their liver were taken. The observed parameters are morphological abnormality including the colour, the contour, ratio between liver weight and body weight as well as histological destruction. The result showed iron dextran treatment was proved the abnormality on morphological and histological desruction. Futhermore, Sappan Wood Extract (Caesalpinia sappan), Wheat grass and Vitamin E Treatment can decrease the morphological abnormality and the liver histological destructionon of iron overload rat. Keywords: Sappan Wood Extract, Wheat grass, Vitamin E, Iron, Morphological abnormality, Histological destruction
The Effectiveness of Antibiotics and Hematopoietic Stem Cell Treatment in Periodontitis Rat Model Toward TNF α Expression
Authors: R Retno Indrawati , Diyatri Indeswati , Rahmawati Dwi
Published: March 2018

Periodontitis is a biofilm-induced chronic inflammatory. The current therapy of periodontitis is scaling root planning and curettage, and followed by administration of antibiotic such as combination of Amoxicillin and Clavulanic acid. Hematopoietic Stem Cell has ability to differentiate into blood cells which is capable of homing and regenerating itself. Research Purpose: The aim of this study was to prove antibiotic and Hematopoietic Stem Cell administration can reduce TNF α expression. Method: This research was divided into four different groups, P1 as negative control group, P2 as positive control group which inoculated with P.gingivalis 109 for three weeks as chronic periodontitis rat model and USP were administered, P3 as chronic periodontitis rat model received Hematopoietic Stem Cell injection into the tail vein of rat, P4 as chronic periodontitis rat models were given Amoxicillin and Clavulanic acid 250 mg/kg BW orally then followed by Hematopoietic Stem Cell 106 injection into the tail vein of rat. After two weeks rat were sacrified and immunohistochemically analysed for expression of TNF α. The data were analysed by using Non-Parametric Test. Result: TNF α expression of negative control group was different as compared to positive control group and treatment group which given Hematopoietic Stem Cell injection, but there is no difference between negative control group with treatment group which given  Amoxicillin and Clavulanic acid 250 mg/kg BW orally and followed by Hematopoietic Stem Cell injection. Clonclusion: The administration of Amoxicillin and Clavulanic acid 250 mg/kg BW and Hematopoietic Stem Cell can reduce TNF α expression on periodontitis rat model.Keyword: Hematopoetic Stem Cell, TNF α, Chronic Periodontitis, Antibiotic
Construction Hybrid immunoglobulin All Four Dengue serotype Using Mesenchymal Stem
Authors: A’la Rofiqul , Ernawati Rahaju , Retno L Nunuk Dyah , Mufasirin Mufasirin , Ma’ruf Anwar , Rantam Fedik A.
Published: March 2018

The dengue virus is a member of vector-borne diseases that causes zoonotic disease and spreads rapidly in the world. No single treatment or vaccine yet is available that is recommended and there is no correlation with protectiveness against this disease. The heavy chain (VH) and light chain (VL) variables are molecules of immunoglobulin G (IgG) is the smallest part of the antibody. Although the part-time domain variable is short, it can be used as a long-term and rapid immune booster in the immune system. In this study we tried to clone an encoding gene that was able to influence the adaptive immune response to dengue 1-4 by using MSC as a gene carrier. The target scFv-IgG gene has been successfully integrated into the plasmid. Plasmids that we have linearly transfected into the MSC. From the cDNA synthesis results continued with PCR synthesis with primer FGHV and RGHA obtained bands in accordance with the target of 404 bp. The scFv gene encoding IgG can be integrated with MSC Keywords: immunetherapy; dengue; hybrid; scFv-IgG; mesenchymal
The Potential of Black Gluten and Red Rice in Rations on the Biological Values and Ideally Body Score of Healthy “Mini Rex Rabbit”
Authors: Sidik Romziah
Published: March 2018

The potentially of black gluten and red rice in rations was observed on performed of biological values and body condition score of Mini Rex Rabbits. Twelve male Mini Rex Rabbits averaging 2 months of age, body weight 1.95 kg were used as experimental animals. Four variations of ration: P0 a commercial, P1, P2, and P3 contains white, red, and black gluten rice, respectively. Research pattern of Complete Randomized Design include four group animals with three replications. The animals were treated by different ration as well as their treatment group during 8 weeks trials, and each animal received Alfalfa hay 10 g/h/d as a basic nutrient. Fecal and urine were collected every day along feeding trials and it’s analyzed by proximate analysis. Body weight and body condition score measured once a week.
Antiviral Activity Effect of Silver Nanoparticles (Agnps) Solution Against the Growth of Infectious Bursal Disease Virus on Embryonated Chicken Eggs with Elisa Test
Authors: Pangestika Rosa , Ernawati Rahaju
Published: March 2018

Infectious bursal disease virus is one of the strategic infectious disease in Indonesia. Despite disinfection and vaccination technology has been doing, the cases still frequently occur and it needs another alternative technology to be developed to against IBD virus. This research try to answer the problem, it examines the effect of antiviral activity of silver nanoparticles (AgNPs) solution against the growth of infectious bursal disease virus in embryonated chicken eggs with ELISA Test. The research has two methods, the first method is conducted by mixing a solution of AgNPs and IBD Virus, two hours before inoculated (preventive method) and the second method is the virus inoculated first, 48 hours later the AgNPs solution injected (therapy method). Each method has several dosage of AgNPs solution respectively 0 ppm (positive control 20 ppm, 40 ppm, and 50 ppm). Virus samples taken from the choriallantoic membrane (CAM) and the embryo by crushed method. Results based on the value of OD (optical density) ELISA Test and Statistical Test ANOVA General Linier Models Univariate with Post-Hoc Duncan 5%, both methods have no significant difference (p>0.05), it means the solution of AgNPs has good preventative and therapeutic characteristic. The mean of OD values also showed dosage of 20ppm is most effective dosage in against the growth of the virus, the dosage has significant difference (p<0.05). The decreasing amount of virus in CAM and in embryos were not significantly different (p>0.05), in both CAM and embryo AgNPs solution has good antiviral properties. Keywords : Silver Nanoparticles, Antiviral, Infectious Bursal Disesase, ELISA, Embryonated Chicken Eggs
Identification and Characterization Indigenous of Lactobacillus spfrom Bovine Rumen Fluidof Slaughterhouse
Authors: Nurhajati Tri , Soepranianondo Koesnoto , Lokapirnasari Widya Paramita , Sahidu Adriana Monica
Published: March 2018

The discovery and characterisation of indigenous lactic acid bacteria (LAB) are important for diversity microbes as candidate probiotic. This research was aimed to identify lactic acid bacteria isolate from isolation process of local bovine rumen fluid from slaughterhouse in Surabaya Indonesia. Genotypic testing was conducted by analyzing 16S rDNA and biochemical identification. DNA of sample isolate was isolated and then amplified in vitro through the PCR method. Determination of nucleotide sequence of 16S rDNA was performed with sequencing method. The result of nucleotide sequence was than compared with GenBank database. The BLAST was then applied to identify the phylogenetic tree. Based on the biochemical characterization and nucleotide sequences, that isolate was identified as Lactobacillus rhamnosus subsp TG15. The result of this research showed that L.rhamnosus subsp TG15 showed viability bacteria in MRSA as control as much as 1.1 x 108 CFU/ml, mean while in MRSA pH 2, L.rhamnosus subsp TG15 showed its viability as much as 9.3 x 106 CFU/ml. Viability of isolate on bile tolerance 0.3% was 2.4 x 107 CFU/ml). Index antagonist bacteria test on S.aureus showed inhibition diameter as much as 2.0 mm and in antagonist test on E.coli as much as 2.5 mm. Based on the result, it could be concluded that this research found a new strain of lactic acid bacteria, L.rhamnosus subsp TG15 and that isolate has ability as the probiotic candidate. Keywords: L.rhamnosus subsp TG15; survival on acidity; bile salts; S.aureus and E.coli
Protective Effect of Propolis Extract Against Lead Acetate Toxicity in Mice (Mus Musculus) Testes
Authors: Widawati Tuti , Sudjarwo Sri Agus , Hermadi Herry Agoes
Published: March 2018

This study was aimed to investigate the protective effect of propolis extract from Apis mellifera that was obtained from Agro Tawon Rimba Raya Malang against the exposure of lead acetate 20 mg/kgBW orally. Twenty-five BALB/C mice were randomly devided into five groups. Negative control received only CMC-Na 1.5 %  and Tween 80 0.5% also aquadest an hour after the first administration; Positive control group that administered CMC-Na 1.5 %  and Tween 80 0.5% then continued received 20 mg/kgBW of lead acetate, Treatment group was received 200; 400 and 800 mg/kgBW of propolis first and then an hour after that received 20 mg/kgBW of lead acetate. The treatment was conducted for 7 days of adaptation and 35 days of treatment. At the end of the research all mice were sacrificed and testes were collected. Testes tissue were processed using Hematoxylin-Eosin staining. The result showed that an increase of spermatocyte, spermatid and sertoli cell compare to group that only received lead acetate, but thickness of seminiferous tubules epithelium showed slighly similar in all groups. Key words  : propolis, lead, reactive oxygen species, free radical, testes
Detection of Antibiotic Residues and Concentration in Raw Milk from Lembang Small Holder Dairy Farm
Authors: Vivi Virgianty
Published: March 2018

The study of “Detection of Antibiotic Residues and Concentration in Raw Milk from Lembang Small Holder Dairy Farms” was held in 3 Tempat Pelayanan Koperasi (TPK) or Cooperative Service Center, members of Koperasi Peternak Sapi Bandung Utara (KPSBU) or Dairy Farmer Cooperative of North Bandung, in Lembang, Kabupaten Bandung, which was chosen purposively, and Unit Pelayanan Teknis Sumber Daya Alam Hayati (UPT SDA Hayati) laboratory in Padjadjaran University at Jatinangor, Sumedang. The subject of this study are to detect qualitative and quantitative possibility of antibiotic presence in raw milk from small holder dairy farms members of KPSBU Lembang.Keywords: Residues, Antibiotic, Raw milk, Residues Concentration, Small Holder Dairy Farms
Potency of Bacillus cereus WPL 415 to Increase Crude Protein and Decrease Crude Fiber of Animal Feed Stuff
Authors: Lokapirnasari Widya Paramita , Sahidu Adriana Monica , Nurhajati Tri , Soepranianondo Koesnoto , Yulianto Andreas Berny
Published: March 2018

This research aims to identify isolate as a probiotic candidate derived from liquor rumen of local beef cattle  and to know the ability of isolates as biofermentor on basal feed to the changes in the nutrient value. The selected samples were obtained from a slaughterhouse in Surabaya.  This study consisted of two stages.  The first stage was the identification of bacteria through the test of morphology, Gram staining, biochemical, resistance to acidity and 16S rDNA sequencing. The second stage was a test of the ability of the isolates on the nutrient of basal feeds by fermentation for three days in an aerobic condition. Based on the findings of the first phase, it has been identified that probiotic bacterium rods, motility positive, Gram-negative, have viability at pH 2 and pH 3 for 90 minutes and 24 hours and have the ability to ferment lactose, sucrose, galactose, ribose, cellobiose and xylose. Furthermore, based on test results of 16S rDNA sequencing, the probiotic bacterium was identified as Bacillus cereus WPL 415. Based on the research results at the second stage, Bacillus cereus WPL 415 at doses of 0.25% and 0.5% could improve the nutrient content of the basal feed. The results of the proximate analysis revealed that there was an increase in crude protein content of 6.78% until 8.12% compared to the control and was able to lower the crude fiber content of 15.19% and 17.40% compared to the control. Based on these results it can be concluded that Bacillus cereus WPL 415 from local beef cattle can be used as a probiotic candidates to improve the quality of animal feed. Keywords: Bacillus cereus, probiotic, crude protein, crude fiber
Acute Toxicity Tests of Alkaloid Pare (Momordica Charanthia) Fruit on The Histopathology of Liver
Authors: Wurlina Wurlina , Meles Dewa Ketut , Zakaria Sunarni , Mustofa Imam , Susilowati Suherni , Adnyana I Dewa Putu Anom
Published: March 2018

Pare fruit (Momordica charanthia) potential as an antidiabetic. In preliminary research has proven that alkaloid of the Pare fruit (Momordica charanthia) can lower blood sugar levels of mice suffering from diabetes mellitus. Alkaloid Pare can improve pancreatic β-cell function by improving the preparation phase for the cell dividing (interphase) and repair the mitotic stages and increase the CDK expression in mice with diabetes mellitus. The purpose of this research was to determine the dose that causes the death of 50% (LD50) and to determine the toxicity effect of alkaloid Pare (Momordica charanthia) against damage in the form of congestion, degeneration, and necrosis of liver cells. Acute toxicity test with a 24-hour long treatment using 60 female mice, divided into six groups, each group consisted of 10 animals. The group is as follows: P0 only given distilled water, the group P1, P2, P3, P4 and P5 respectively treated 0.3 g/kg bw, 1 g/kg bw, 3 g/kg bw, 10 g/kg bw and 15 g/kg bw. Furthermore, all the mice were necropsied to take the liver for histopathology preparations to observe the occurrence of congestion, degeneration, and necrosis. The parameters observed through histopathological examination of the liver include congestion, degeneration, and necrosis. The results of the research tested by Kruskal-Wallis test with data obtained based on the value of scoring was not significantly different (α<0.05) in the liver histopathological changes in the form of congestion, degeneration, and necrosis between control and treatment groups orally in time 24 hours. The dose can cause 50% of deaths more than 15 g in mice are included in the category of medicinal substances which are not harmful.    Keywords:  Alkaloid Pare fruit, LD50, congestion, degeneration, necrosis, liver.
Teratogenic Effect of Congenital Toxoplasmosis in Chicken Embryo
Authors: Suwanti Lucia Tri , Mufasirin Mufasirin , Plumeriastuti Hani , Safitri Erma
Published: March 2018

This research is designed to observe the teratogenic effect of Toxoplasma gondii infection in chick embryos, based on the number of somites, embryo length and the development of embryonic brain vesicles. Methods in the research: Chicken eggs were infected with 1 x 103 tachyzoites of T. gondii. The eggs were incubated in eggs hatching box. Observation of somite performed on embryonated eggs 24 hours after incubation and the embryonic development of vesicles performed 72 hours after incubation then the length of each embryo were measured. Results: Revealed that there was a significant difference in the number of somites (p < 0.1), T. gondii infection reduced the number of somites. While in the number of brain vesicles in 3 - days old chicken embryos, although there was no significant difference, the size declining emerged. The length of the embryos both at 24 or 72 hours old showed that T. gondii infection reduced the length (p < 0.1). Conclusions: T. gondii infection influences the development of chicken embryos in the declining of length and the decreasing of somite embryo number. Keywords: IGF-I crossbreed mare serum pregnant; Follicle; Mus musculus
Case Study : Dystocia on Beef Cattle in Kunir Regency of Lumajang District, East Java, Indonesia in 2015 and 2016
Authors: Febrianila Rosiana , Lokapirnasari Widya P , Restiadi Tjuk I , Mustofa Imam , Hermadi Herry A , Safitri Erma
Published: March 2018

Dystocia defined as difficulty of birth. Cattle that experienced dystocia characterized by extended processing time of birth, difficult, and impossible to do without help of human stem. This study aimed to determine the number and causative factor of dystocia in beef cattle in Kunir sub district, Lumajang district. Data acquisition used primary data and secondary data. Primary data was obtained from direct observations about management of maintenance, then interviewed the farmers in Kunir sub district. Secondary data was data obtained from the recording belongs to animal health technical officer. The results showed that the prevalence of dystocia in Kunir district, Lumajang district as many as 63 cases or 11,6 % of 543 births. The result of the research is analyzed using chi square (χ2) method on SPSS 20.0 program and risk factors that increase the incident of dystocia were IB semen which greater than the cattle site, the position of the fetus and inertia uteri can result in weakness of the catrle at the age of older and more likely to give birth. Key words: Dystocia; beef catlle
The Effectiveness of Honey in Physiological Nacl to Maintain of Viability and Motility of Spermatozoa
Authors: Agustina Elsa , Hermadi Herry Agoes , S Hario Puntodewo , Hernawati Tatik , Triana Indah Norma , Safitri Erma
Published: March 2018

This research was conducted to determine whether there is influence of various concentrations of mead physiological saline as diluent on the viability and motility fat-tailed sheep during storage 5°C. This research is compiled using the trial completely randomized design (CRD). Differences in the treatment of thinning the honey in physiological saline are PO (0%), P1 (0.2%), P2 (0.4%), P3 (0.6%), P4 (0.8%). The treatment  stored in a refrigerator at a temperature of 5°C and then observed every 0, 24, and 48 hours. Data were analyzed by ANOVA followed by Duncan's Multiple Range Test. The  conclusions obtained from this research there is the effect of adding various concentrations of NaCl physiological mead on the viability and motility fat tailed sheep during storage 5°C and consentration mead NaCl physiological to maintain viability and motility best tail sheep are treated P3 (0.6% solution = 0.6 ml honey in 99.4 ml physiological NaCl) at the time T24 (24 hours).  Key words: Fat-Tailed Sheep, Honey, Motility, Physiological NaCl, Spermatozoa, Viability
Utilization of Sumbawa Tropical Forest Honey Apis Dorsata to Improve Fertility of Indonesia Oriental Magpie Robin (Copsychus saularis) as Effort Animal Population Increasement
Authors: Hasib Abdullah , Muhamad Risaldi , Reksa Talita Yuanda , Artha Alvina Ulimaz , Safitri Erma
Published: March 2018

The objective of this experiment is to observe the effect of forest honey produced by giant bees Apis dorsata on improving fertility and egg number of the bird. The bird which observed in this experiment is captive bird Oriental Magpie Robin (Copsychus saularis). Twelve paired captive bird in official captivity were transferred into new pairing cage and adapted along two weeks. Every bird was fed with crickets and normal ration mixed with low protein diet (LP) with 16% of crude protein gradually. After an adaptation period, twelve paired captive bird were divided into three groups T1, T2 and T3. Birds were treatened with honey solution 5%, 10% and 15% concentration series in 1 ml aquadest intraabdominal of 10 crickets and fed with 15 crickets and 20 gram low protein ration (16%) per day for two laying periods.  Egg production each group were calculated and observed its fertility among all egg production in one parental. In accordance with that, the results were analyzed descriptively. This pre-treatment, the bird’s mating behaviour becomes a week faster than the normal period. Bird’s fertility is also increasing with the provision of honey in the bird nutrition. In the bird’s pairs in cage’s number 9 and 10 have shown development by producing 3 fertile eggs after being treated with honey. In the previous period showed from 3 eggs only 1 being fertile. Honey has not been able to increase the number of eggs produced by birds. However, there is an enhancement in the animal population up to 25% of the 12 pairs of birds that are used as experimental samples in the captivity. The conclusion of this study shows that honey can accelerate the mating behaviour of birds and can increase the fertility of birds' eggs. However, honey has not yet affected the increase the quantity of the birds' egg. Longer observation’s duration with extra bird’s sample is needed for future research, in order to see the reproduction cycle of birds over several periods. Field research related to nutrition in chemistry nutrition’s fields related the active ingredient in honey that can affect the reproduction system is also important to conducted for further research. Keywords: Oriental Magpie Robin; Eggs Production; Fertility; Mating Behaviour; Honey
Utilization of Honey Apis dorsata as Antiosteoporosis on Requirements of Bone Calcium Ash Density on Ovariohysterectomized White Rat (Ratus norvegicus)
Authors: Ibrahim Muhammad Huda Ramadhan , Hasib Abdullah Hasib , Rohmah Siti Nur , Abani Salsabilla , Yordan Samsi , Yudaniayanti Ira Sari
Published: March 2018

The purpose of this study was to determine the effect of honey bee Apis dorsata as anti-osteoporosis in calcium ash density (CAD) of bone in osteoporotic-induced rats. The target of this study was to know bone calcium levels after being given honey bees Apis dorsata. In this study, 35 female white rats (Ratus norvegicus) mature was used with weight 200gr. Divided into 5 groups, 2 control groups and 3 treatment groups. The negative control group (SH) was not induced by osteoporosis and was given the only aquadest of 1.5 ml/day. Whereas the positive control group was induced by osteoporosis (OH)  and was given only aquadest 1.5 ml/day. T1, T2 and T3 treatment groups were given bee honey with various doses including 1g / kg ad 1.5 ml aquadest, 2g / kg BB ad 1.5 ml aquadest and 3g / kg BB ad 1.5 ml aquadest. Then after 12 weeks, white rats were sacrificed for lumbar vertebrae. Furthermore, the sixth lumbar spine os vertebrae will be examined for calcium bone ash content. The data were obtained was analyzed using statistical analysis of variance (ANOVA) for the results of the calcium ash content data Keywords: Honey; Osteoporosis; CAD
Increased Integrity of Plasma Membrane and Acrosome Cap Spermatozoa Limousin Cattle at Post Thawing in Frozen Media by adding Seawater Extract
Authors: Faidah Nur , Hernawati Tatik , Lamid Mirni , Ismudiono Ismudiono , Suprayogi Tri Wahyu , Mulyati Sri
Published: March 2018

This research was determined membrane integrity and acrosome cap of Limousin bull post thawing after adding seawater extract with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research used fresh samples of Limousin bull’s semen collected by using artificial vagina, then devided into 4 treatments and 6 replications. The experimental design that used was Complete Random Design. Analysis of the data using Analysis of Variant (ANOVA) one way then proceeds to the Duncan Multiple Range Test to determine significant differences between treatments.The first treatment P0 no seawater extract added as a control. The second treatment P1 was treated with 0.109 µL seawater extract, P2 was treated with 0.426 µL seawater extract, and P3 was treated with 1.09 µL seawater extract. The result showed that determine significant differences between treatments. The post thawing membrane integrity’s result was P0= 22.00 ± 4.28, P1= 22.66 ± 3.61, P2= 25.00 ± 2.75, and P3= 29.00 ± 1.67. The post thawing acrosome cap’s result was P0= 30.50 ± 1.37, P1= 31.50 ± 3.27, P2= 34.83 ± 2.31, and P3= 38.00 ± 1.41. The highest concentration added seawater extract to increased membrane integrity and acrosome cap spermatozoa in this research was 1.09 µL. Keywords: Limousin bull; spermatozoa; seawater extract; membrane integrity; acrosome cap
The Relation of Body Temperature and Vaginal Cytology Examination in Time Artificial Insemination Rate Fat-tailed Sheep (Ovis Aries) in The District Sidoarjo East Java
Authors: Anggriawan Rhendyka Prasetya , Utama Suzanita , Eliyani Hana
Published: March 2018

Ewes fertility is one aspect that needs to maximum level. Reproductive performance in sheep livestock in Indonesia has become one of the main determinants of reproductive success. This study has been carried out of the body temperature measurement and sampling of vaginal cytology of 60 fat-tailed sheep before artificial insemination in Sidoarjo East Java. Body temperature measurement is done in the rectum of reported ewes in heat and artificial insemination requested can be determined by observing the condition of the vulva of ewes such as the presence or absence of mucus through the vagina, vulva swollen and reddened vulva mucosa. vaginal cytology sampling with a cotton swab inserted into the vulva ± 15cm of ewes that reported in heat then interpreted by a microscope. The result showed that ewe’s body temperature in 39.1oC-39.2oC has a significant correlation with vaginal cytology type on oestrous phase. Keyword: Ewes, body temperature, vaginal cytology, the district Sidoarjo East Java
Effect of Laser Acupuncture Shoot on Ova Point of Male Mojosari Duck (Anas plathyrhynchos) on The Number of Sertoli and Leydig Cells
Authors: Adikara Yuanara AR , Samik Abdul , Yudaniayanti Ira S , Adikara Tatang S , Hestianah Eka P , Utama Suzanita
Published: March 2018

Demand for duck eggs and meat recently increased along with the increasing interest of the community to consume duck eggs and meat. Due to that reason researcher try to find the best way to enhance the reproductive ability of livestock., which is in this research by laser puncture shot. Eighteen ducks were allocated into three groups, the control group, the first treatment group with laserpuncture shoot dose of 0.2 Joule (J), and the second treatment group with laser puncture shoot dose of 0.4 J. Data were analyzed with ANOVA and further tested by Duncan multiple range test. Research result showed that laser puncture shoot with a dose of 0.2 J and 0.4 J decreased the number of Sertoli and Leydig cells.  Keywords: Mojosari duck; laser acupuncture; Sertoli cells; Leydig cells
Insulin-Like Growth Factor-I (IGF-I) from Crossbred Pregnant MareSerum to Increase Follicle Number of Mice (Mus musculus)
Authors: Abdullah Abdullah , IRestiadi Tjuk , Lastuti Nunuk DR , Damayanti Tita , Wurlina Wurlina , Safitri Erma
Published: March 2018

The purpose of the research was to know the effect of Insulin-Like Growth Factor-I (IGF-I) derived from pregnant crossbred mare serum (PMS) in mice (Mus musculus) folliculogenesis. The subjects of this research were 20 female mice. The research was arranged by Completely Randomized Design (CRD) with four treatments and five replications. The treatments were C0 = 10 ng/ml of physiological NaCl, P1 = 10 ng/ml of IGF-I PMS, P2 = 20 ng/ml of IGF-I PMS, and P3 = 40 ng/ml of IGF-I PMS. Observed variables are the number of primary, secondary, tertiary and de Graff follicles. During the treatment, the estrous cycle was also observed. The data of follicles number were analyzed by Analysis of Variance (ANOVA) and followed by HSD (Honestly Significant Difference) test. The result showed that the addition of IGF-I PMS significantly affects (p<0.05) on increasing the primary and secondary follicles number. The addition of IGF-I PMS 20 ng/ml and 40 ng/ml can increase the primary and secondary follicles significantly (p<0.05).  Keywords: IGF-I crossbreed mare serum pregnant; follicle; Mus musculus
Morphological Identification Nematodes Tanqua tiara Found on Gastric Varanus salvator at East Java
Authors: Agustin Alfiana Laili Dwi , Koesdarto Setiawan , Lukiswanto Bambang Sektiari , Suwanti Lucia Tri , Arifin Zainal , Putranto Emmanuel Djoko
Published: March 2018

Tanqua tiara (T. tiara) is the gastric nematode of Varanus salvator (V. salvator) [1-4], this study was conducted to identification morphology of T. tiara. Although there are no reports of human and animal infection T.tiara but still have the potential to transmit the disease to humans through direct contact or indirectly. Isolation of adult worms T.  tiara from the digestive tract of V. salvator and then will make to dry preparat.Some 20 V.alvator's gastrointestinal tract at autopsy and gained as much as 321 nematode worms, nematode worms were obtained from the stomach V. salvator, result identification from dry preparat show : Male (mm) (Total length of body 9.4-32, Wide body 0,26-1,77, Diameter of head-bulb 0,17-0,32, The length of the head 0,23-0,33, Distance from the head-end to the end of the esophagus 2,8-5,7, Distance from the head-end to cervical sac 0,22-0,81, The thickness of the cuticle 0,008-0,036, Long tail 0,13-0,42, Long spikula0,3-1,1) and female (mm) (Total length of body 6,8-22, Wide body 0,14-2,33, Diameter of head-bulb 0,15-0,34, The length of the head 0,1-0,28, Distance from the head-end to the end of the esophagus 2,3-4,58, Distance from the head-end to cervical sac 0,18-0,88, The thickness of the cuticle 0,007-0,031, Long tail 0,12-0,28, Distance vulva from the tail end 3,02-3,61, Long uterus 13,1-21,4, size of egg 0,04-0,052X0,03-0,049). Identification of morphological Tanqua tiara indicate worms vary in size, but the specimen is dominated by small-sized worms.  Keywords: Varanus salvator; Tanqua tiara; Cephalic bulb.
Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferous Tubules in Male Mice (Mus musculus)
Authors: Nurkarimah Dona Astari , Hestianah Eka Pramyrtha , Wahjuni Retno Sri , Hariadi Mas’ud , Kuncorojakti Suryo , Hermadi Herry Agoes
Published: March 2018

The present research aimed to determine the effect of propolis in spermatogenic cells number and seminiferous tubules diameter. Group P0 served as control group, P1 (1,6 mg/0,5ml/day), P2 (3,2 mg/0,5 ml/day), P3 (6,4 mg/0,5 ml/day), and P4 (12,8 mg/0,5 ml/day) were given propolis ethanolic extract treatment orally and killed after 14 days. Spermatogenic cells number (spermatogonial cells, primary spermatocytes, spermatids) and seminiferous tubules diameter were observed. The result showed a lower number of spermatogonial in P1 and P2 groups, primary spermatocytes reduction in P2 group, spermatids increased in P1, P3, and P4 group. Seminiferous tubules diameter decreased in P2 and P3 group. P2 group (3,2 mg/0,5 ml/day) showed the lowest result in all parameters (p<0.05). However, oral administration of propolis at these dose for 14 days may decrease spermatogonial cells and primary spermatocytes and increase spermatids number in adult mice.  Keywords: Propolis; seminiferous tubule; spermatogenic cells; testis histology
The Effect of Blue Green Algae (Spirulina platensis) Extract in White Rat (RattusNorvegicus) Treated with Excessive Physical Exercise on Leydig Cell Number and Seminiferous Tubules Diameter
Authors: Lazuardi Dimas Yuzrifar Rhavindra , Rimayanti Rimayanti , Primarizky Hardany , Sudjarwo Sri Agus , Utama Suzanita , Rachmawati Kadek
Published: March 2018

The aim of this study was to investigate the effect of Spirulina platensis extract on the amount of Leydig cells, amount of necrotic Leydig cells, and diameter of seminiferous tubules of rats treated with excessive physical exercise. The excessive physical exercise was done through 60 minutes of swimming for 35 days. Twenty white male rats were randomly divided into five groups i.e C-, control group, C+, a group was only receive swimming, T1, T2, and T3 was received 300, 600, and 1200 mg/kg BW of Spirulina platensis and swimming. Spirulina platensis extract was given orally once a day before swimming. ANOVA followed by Duncan analysis showed significant differences among treatments. Excessive physical exercise influenced on male reproduction system through decreasing the amount of Leydig cells. The conclusion was a dose of 1200 mg/kg BW of Spirulina platensis extract could maintain the amount of Leydig cells, protected the Leydig cells from becoming necrotic, and increased the diameter of seminiferous tubules. Keywords: Rattus Norvegicus, Spirulinaplatensis, excessive physical exercise, Leydig cells, seminiferous tubules diameter
The Effect of Mahkota Dewa (Phaleria macrocarpa) Pulp Extract by Peroral Administration Toward The Percentage of Capacitation and Acrosome Reaction in Rat (Rattus norvegicus)
Authors: Bagyo Dhanang Estu , Utomo Budi , Setiabudi Rudy Sukamto
Published: March 2018

This research was conducted to find outthe influence of mahkota dewa (Phaleria macrocarpa) pulp extract against the percentage of capacitation and acrosome reaction of male rat (Rattus norvegicus). The experimental animals used are 20 male rats with the average body weight of 200g and were divided into four different groups. P0 group was the control group and given only CMCNa 0.5%. P1, P2,and P3 was given mahkota dewa pulp extract with a dosage of 7.5 mg/200gr BW/ day, 15mg/200gr BW/day,and 30 mg/200gr BW/day respectively through peroral administration for 14 days. The research used Completely Randoized Design (CRD) and the data was then analyzed by one way ANOVA (Analysis of Variance) followed by Duncan test with the significance of 0.5. The results show that the mahkota dewa pulp extract shows significant impact on capacitation and acrosome reaction of rat. P2 group shows the highest percentage capacitation significance the a mean of 30.00 ± 4.24 and 5.60 ± 0.89 for acrosome reaction percentage.  Keywords: Phaleria macrocarpa pulp extrtact; capasitation; acrosome reaction; sperm potention