bluuebunny/crossref_metadata_2025 huggingface dataset:
SuperHistopath: A Deep Learning Pipeline for Mapping Tumor Heterogeneity on Low-Resolution Whole-Slide Digital Histopathology Images
Authors: Zormpas-Petridis Konstantinos , Noguera Rosa , Ivankovic Daniela Kolarevic , Roxanis Ioannis , Jamin Yann , Yuan Yinyin
Published: January 2021

High computational cost associated with digital pathology image analysis approaches is a challenge towards their translation in routine pathology clinic. Here, we propose a computationally efficient framework (SuperHistopath), designed to map global context features reflecting the rich tumor morphological heterogeneity. SuperHistopath efficiently combines i) a segmentation approach using the linear iterative clustering (SLIC) superpixels algorithm applied directly on the whole-slide images at low resolution (5x magnification) to adhere to region boundaries and form homogeneous spatial units at tissue-level, followed by ii) classification of superpixels using a convolution neural network (CNN). To demonstrate how versatile SuperHistopath was in accomplishing histopathology tasks, we classified tumor tissue, stroma, necrosis, lymphocytes clusters, differentiating regions, fat, hemorrhage and normal tissue, in 127 melanomas, 23 triple-negative breast cancers, and 73 samples from transgenic mouse models of high-risk childhood neuroblastoma with high accuracy (98.8%, 93.1% and 98.3% respectively). Furthermore, SuperHistopath enabled discovery of significant differences in tumor phenotype of neuroblastoma mouse models emulating genomic variants of high-risk disease, and stratification of melanoma patients (high ratio of lymphocyte-to-tumor superpixels (p = 0.015) and low stroma-to-tumor ratio (p = 0.028) were associated with a favorable prognosis). Finally, SuperHistopath is efficient for annotation of ground-truth datasets (as there is no need of boundary delineation), training and application (~5 min for classifying a whole-slide image and as low as ~30 min for network training). These attributes make SuperHistopath particularly attractive for research in rich datasets and could also facilitate its adoption in the clinic to accelerate pathologist workflow with the quantification of phenotypes, predictive/prognosis markers.
Cancer Care of Children, Adolescents and Adults With Autism Spectrum Disorders: Key Information and Strategies for Oncology Teams
Authors: Vuattoux Delphine , Colomer-Lahiguera Sara , Fernandez Pierre-Alain , Jequier Gygax Marine , Choucair Marie-Louise , Beck-Popovic Maja , Diezi Manuel , Manificat Sabine , Latifyan Sofiya , Ramelet Anne-Sylvie , Eicher Manuela , Chabane Nadia , Renella Raffaele
Published: January 2021

Delivering optimal cancer care to children, adolescents and adults with ASD has recently become a healthcare priority and represents a major challenge for all providers involved. In this review, and after consideration of the available evidence, we concisely deliver key information on this heterogenous group of neurodevelopmental disorders, as well as recommendations and concrete tools for the enhanced oncological care of this vulnerable population of patients.
Targeted Chinese Medicine Delivery by A New Family of Biodegradable Pseudo-Protein Nanoparticles for Treating Triple-Negative Breast Cancer: In Vitro and In Vivo Study
Authors: Kwan Hiu Yee , Xu Qinghua , Gong Ruihong , Bian Zhaoxiang , Chu Chih-Chang
Published: January 2021

Triple negative breast cancer (TNBC) has the worst overall survival among all breast cancer subtypes; 80% of TNBC harbors TP53 mutation. Gambogic acid (GA) is an herbal compound isolated from the dry brownish gamboge resin of Garcinia hanburyi. A new family of biodegradable polymer, the folate (FA)-conjugated arginine-based poly(ester urea urethane)s nanoparticles (FA-Arg-PEUU NP), was developed as nano-carrier for GA. Its anti-TNBC effects and the underlying mechanism of action were examined. The average diameters of FA-Arg-PEUU NP and GA-loaded FA-Arg-PEUU NP (NP-GA) in water are around 165 and 220nm, respectively. Rhodamine-tagged FA-Arg-PEUU NP shows that the conjugation of FA onto Arg-PEUU NPs facilitates the internalization of FA-Arg-PEUU-NP into TNBC. Compared to free-GA at the same GA concentrations, NP-GA exhibits higher cytotoxicity in both TP53-mutated and non-TP53 expressed TNBC cells by increasing intrinsic and extrinsic apoptosis. In HCC1806-bearing xenograft mouse model, the targeted delivery of GA by the FA-Arg-PEUU-NP nano-carriers to the tumor sites results in a more potent anti-TNBC effect and lower toxicity towards normal tissues and organs when compared to free GA. Furthermore, NP-GA also reduces the tumor-associated macrophage (TAM) M1/M2 ratio, suggesting that the use of Arg-based nanoparticles as carriers for GA not only makes the surface of the nanoparticles positively charged, but also confers on to the nanoparticles an ability to modulate TAM polarization. Our data clearly demonstrate that NP-GA exhibits potent anti-TNBC effects with reduced off-target toxicity, which represents novel alternative targeted therapeutics for TNBC treatment.
Bcl9 Depletion Modulates Endothelial Cell in Tumor Immune Microenvironment in Colorectal Cancer Tumor
Authors: Wei Zhuang , Feng Mei , Wu Zhongen , Shen Shuru , Zhu Di
Published: January 2021

Tumor endothelial cells are an important part of the tumor microenvironment, and angiogenesis inhibitory therapy has shown potential in tumor treatment. However, which subtypes of tumor endothelial cells are distributed in tumors, what are the differences between tumor endothelial cells and normal endothelial cells, and what is the mechanism of angiogenesis inhibitory therapy at the histological level, are all need to be resolved urgently. Using single-cell mRNA sequencing, we analyzed 12 CT26 colon cancer samples from mice, and found that knockdown of the downstream factor BCL9 in the Wnt signaling pathway or inhibitor-mediated functional inhibition can modulate tumor endothelial cells at a relatively primitive stage, inhibiting their differentiation into further extracellular matrix construction and angiogenesis functions. Furthermore, we propose a BCL9-endo-Score based on the differential expression of cells related to different states of BCL9 functions. Using published data sets with normal endothelial cells, we found that this score can characterize endothelial cells at different stages of differentiation. Finally, in the The Cancer Genome Atlas (TCGA) pan-cancer database, we found that BCL9-endo-Score can well predict the prognosis of diseases including colon cancer, kidney cancer and breast cancer, and identified the markers of these tumor subtypes, provide a basis for the prognosis prediction of patients with such types of tumor. Our data also contributed knowledge for tumor precision treatment with angiogenesis inhibitory therapy by targeting the Wnt signaling pathway.
Proton Beam Therapy for Children With Neuroblastoma: Experiences From the Prospective KiProReg Registry
Authors: Jazmati Danny , Butzer Sarina , Hero Barbara , Ahmad Khalil Dalia , Merta Julien , Bäumer Christian , Plum Gina , Fuchs Jörg , Koerber Friederike , Steinmeier Theresa , Peters Sarah , Doyen Jerome , Thole Theresa , Schmidt Matthias , Blase Christoph , Tippelt Stephan , Eggert Angelika , Schwarz Rudolf , Simon Thorsten , Timmermann Beate
Published: January 2021

Objective

Radiotherapy (RT) is an integral part of the interdisciplinary treatment of patients with high-risk neuroblastoma (NB). With the continuous improvements of outcome, the interest in local treatment strategies that reduce treatment-related side effects while achieving optimal oncological results is growing. Proton beam therapy (PBT) represents a promising alternative to conventional photon irradiation with regard to the reduction of treatment burden.

Method

Retrospective analysis of children with high or intermediate risk NB receiving PBT of the primary tumor site during first-line therapy between 2015 and 2020 was performed. Data from the prospective in-house registry Standard Protonentherapie WPE – Kinder- (KiProReg) with respect to tumor control and treatment toxicity were analyzed. Adverse events were classified according to CTCAE Version 4 (V4.0) before, during, and after PBT.

Results

In total, 44 patients (24 male, 20 female) with high (n = 39) or intermediate risk NB (n = 5) were included in the analysis. Median age was 3.4 years (range, 1.4–9.9 years). PBT doses ranged from 21.0 to 39.6 Gray (Gy) (median 36.0 Gy). Five patients received PBT to the MIBG-avid residual at the primary tumor site at time of PBT according to the NB-2004 protocol. In 39 patients radiation was given to the pre-operative tumor bed with or without an additional boost in case of residual tumor. After a median follow-up (FU) of 27.6 months, eight patients developed progression, either local recurrence (n = 1) or distant metastases (n = 7). Four patients died due to tumor progression. At three years, the estimated local control, distant metastatic free survival, progression free survival, and overall survival was 97.7, 84.1, 81.8, and 90.9%, respectively. During radiation, seven patients experienced higher-grade (CTCAE ≥ °3) hematologic toxicity. No other higher grade acute toxicity occurred. After PBT, one patient developed transient myelitis while receiving immunotherapy. No higher grade long-term toxicity was observed up to date.

Conclusion

PBT was a well tolerated and effective local treatment in children with high and intermediate risk NB. The role of RT in an intensive multidisciplinary treatment regimen remains to be studied in the future in order to better define timing, doses, target volumes, and general need for RT in a particularly sensitive cohort of patients.
Neurodevelopment Outcome of Neonates Treated With Intraventricular Colistin for Ventriculitis Caused by Multiple Drug-Resistant Pathogens—A Case Series
Authors: Hussain Kashif , Sohail Salat Muhammad , Ambreen Gul , Iqbal Javaid
Published: January 2021

Background:Multiple-drug-resistant Gram-negative bacteria (MDR-GNB)-associated neonatal ventriculitis is a life-threatening complication that needs timely diagnosis and effective treatment with broad-spectrum antimicrobials in critical-care settings. Inadequate penetration of antibiotics through the blood–brain barrier also demands an intraventricular (IVT) route of administration. This study reports mortality and neurodevelopmental sequelae of neonates till 18 months of age, who received IVT-colistin for treating MDR-GNB associated ventriculitis.

Methods:In a case series of seven neonates with ventriculitis due to MDR-GNB at NICU of Aga Khan University Hospital, Pakistan, between June 2015 and 2018, we reviewed IVT-colistin therapy in critically ill neonates. Treatment outcomes were assessed based on clinical sign's resolution and MDR-GNB eradication in subsequent CSF cultures. Neurodevelopmental outcomes were evaluated at 18 months after discharge.

Results:The average birth weight was 1.38 kg (range: 1.02–1.5 kg), and the average gestational age was 30.7 weeks (ranged: 26–34 weeks). All neonates reported colistin-sensitive MDR-GNB in CSF, five withAcinetobacter baumannii, and polymicrobial CNS infection was found in two patients (one due toKlebsiella pneumoniaandA. baumanniiand one dueto K. pneumoniaandEscherichia coli). All neonates received IVT colistin and concomitant intravenous meropenem, and five of them also received intravenous colistin. One neonate died. At the 18-month assessment, only one neonate had cerebral palsy and hydrocephaly and 50% had seizure disorders.

Conclusion:Practicing intraventricular antibiotics in the neonatal population is challenging but may be used successfully, especially to overcome the limitation of poor penetration through the blood–brain barrier.
Functional Constipation and the Gut Microbiome in Children: Preclinical and Clinical Evidence
Authors: Avelar Rodriguez David , Popov Jelena , Ratcliffe Elyanne M. , Toro Monjaraz Erick Manuel
Published: January 2021

Functional constipation is a common condition in childhood with significant impact on patients' quality of life and on health care resources. Functional constipation is characterized by decreased bowel movements and/or hard stools, which cause significant distress for children and their caregivers. While the term “functional” may imply the absence of organic causes with a focus on behavioral aspects, 40% of children continue to have symptoms beyond conventional management with one in four children continuing to experience constipation into adulthood. The refractory and chronic nature of constipation highlights the importance of considering a range of pathophysiological mechanisms, including the potential role of the gut microbiome. In this review, we provide an overview of preclinical and clinical studies that focus on the potential mechanisms through which the gut microbiome might contribute to the clinical presentation of functional constipation in pediatrics.
Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Authors: Pohjolainen Lotta , Easton Julia , Solanki Reesha , Ruskoaho Heikki , Talman Virpi
Published: January 2021

Background: Hypertrophy of cardiomyocytes (CMs) is initially a compensatory mechanism to cardiac overload, but when prolonged, it leads to maladaptive myocardial remodeling, impairing cardiac function and causing heart failure. A key signaling molecule involved in cardiac hypertrophy is protein kinase C (PKC). However, the role of different PKC isoforms in mediating the hypertrophic response remains controversial. Both classical (cPKC) and novel (nPKC) isoforms have been suggested to play a critical role in rodents, whereas the role of PKC in hypertrophy of human CMs remains to be determined. Here, we aimed to investigate the effects of two different types of PKC activators, the isophthalate derivative HMI-1b11 and bryostatin-1, on CM hypertrophy and to elucidate the role of cPKCs and nPKCs in endothelin-1 (ET-1)-induced hypertrophy in vitro.

Methods and Results: We used neonatal rat ventricular myocytes (NRVMs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to study the effects of pharmacological PKC modulators and ET-1. We used quantitative reverse transcription PCR to quantify hypertrophic gene expression and high-content analysis (HCA) to investigate CM morphology. In both cell types, ET-1, PKC activation (bryostatin-1 and HMI-1b11) and inhibition of cPKCs (Gö6976) increased hypertrophic gene expression. In NRVMs, these treatments also induced a hypertrophic phenotype as measured by increased recognition, intensity and area of α-actinin and F-actin fibers. Inhibition of all PKC isoforms with Gö6983 inhibited PKC agonist-induced hypertrophy, but could not fully block ET-1-induced hypertrophy. The mitogen-activated kinase kinase 1/2 inhibitor U0126 inhibited PKC agonist-induced hypertrophy fully and ET-1-induced hypertrophy partially. While ET-1 induced a clear increase in the percentage of pro-B-type natriuretic peptide-positive hiPSC-CMs, none of the phenotypic parameters used in HCA directly correlated with gene expression changes or with phenotypic changes observed in NRVMs.

Conclusion: This work shows similar hypertrophic responses to PKC modulators in NRVMs and hiPSC-CMs. Pharmacological PKC activation induces CM hypertrophy via activation of novel PKC isoforms. This pro-hypertrophic effect of PKC activators should be considered when developing PKC-targeted compounds for e.g. cancer or Alzheimer’s disease. Furthermore, this study provides further evidence on distinct PKC-independent mechanisms of ET-1-induced hypertrophy both in NRVMs and hiPSC-CMs.
Network Patterns of Herbal Combinations in Traditional Chinese Clinical Prescriptions
Authors: Wang Ning , Du Ninglin , Peng Yonghong , Yang Kuo , Shu Zixin , Chang Kai , Wu Di , Yu Jian , Jia Caiyan , Zhou Yana , Li Xiaodong , Liu Baoyan , Gao Zhuye , Zhang Runshun , Zhou Xuezhong
Published: January 2021

As a well-established multidrug combinations schema, traditional Chinese medicine (herbal prescription) has been used for thousands of years in real-world clinical settings. This paper uses a complex network approach to investigate the regularities underlying multidrug combinations in herbal prescriptions. Using five collected large-scale real-world clinical herbal prescription datasets, we construct five weighted herbal combination networks with herb as nodes and herbal combinational use in herbal prescription as links. We found that the weight distribution of herbal combinations displays a clear power law, which means that most herb pairs were used in low frequency and some herb pairs were used in very high frequency. Furthermore, we found that it displays a clear linear negative correlation between the clustering coefficients and the degree of nodes in the herbal combination network (HCNet). This indicates that hierarchical properties exist in the HCNet. Finally, we investigate the molecular network interaction patterns between herb related target modules (i.e., subnetworks) in herbal prescriptions using a network-based approach and further explore the correlation between the distribution of herb combinations and prescriptions. We found that the more the hierarchical prescription, the better the corresponding effect. The results also reflected a well-recognized principle called “Jun-Chen-Zuo-Shi” in TCM formula theories. This also gives references for multidrug combination development in the field of network pharmacology and provides the guideline for the clinical use of combination therapy for chronic diseases.
Targeting Neurological Manifestations of Coronaviruses by Candidate Phytochemicals: A Mechanistic Approach
Authors: Fakhri Sajad , Piri Sana , Majnooni Mohammad Bagher , Farzaei Mohammad Hosein , Echeverría Javier
Published: January 2021

The novel coronavirus 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made a wide range of manifestations. In this regard, growing evidence is focusing on COVID-19 neurological associations; however, there is a lack of established pathophysiological mechanisms and related treatments. Accordingly, a comprehensive review was conducted, using electronic databases, including PubMed, Scopus, Web of Science, and Cochrane, along with the author’s expertize in COVID-19 associated neuronal signaling pathways. Besides, potential phytochemicals have been provided against neurological signs of COVID-19. Considering a high homology among SARS-CoV, Middle East Respiratory Syndrome and SARS-CoV-2, revealing their precise pathophysiological mechanisms seems to pave the road for the treatment of COVID-19 neural manifestations. There is a complex pathophysiological mechanism behind central manifestations of COVID-19, including pain, hypo/anosmia, delirium, impaired consciousness, pyramidal signs, and ischemic stroke. Among those dysregulated neuronal mechanisms, neuroinflammation, angiotensin-converting enzyme 2 (ACE2)/spike proteins, RNA-dependent RNA polymerase and protease are of special attention. So, employing multi-target therapeutic agents with considerable safety and efficacy seems to show a bright future in fighting COVID-19 neurological manifestations. Nowadays, natural secondary metabolites are highlighted as potential multi-target phytochemicals in combating several complications of COVID-19. In this review, central pathophysiological mechanisms and therapeutic targets of SARS-CoV-2 has been provided. Besides, in terms of pharmacological mechanisms, phytochemicals have been introduced as potential multi-target agents in combating COVID-19 central nervous system complications.
Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro
Authors: Li Ting , Fang Ting , Xu Linxin , Liu Xiangyang , Li Xiaoyu , Xue Mei , Yu Xiaochen , Sun Bei , Chen Liming
Published: January 2021

Background: Metabolic associated fatty liver disease (MAFLD), characterized by hepatic lipid accumulation and fatty degeneration, is intertwined with obesity and type 2 diabetes mellitus (T2DM). Empagliflozin is a sodium-glucose cotransporter-2 inhibitor that effectively lowers blood glucose, but its effect on MAFLD and associated mechanisms are not fully understood.

Methods: Eight-week-old db/db mice, an in vivo model, were administered empagliflozin or saline intragastrically. A hepatocyte steatosis model was established by inducing HL7702 cells with high glucose and palmitic acid and then treated with or without empagliflozin. The autophagy inhibitor (3-methyladenine, 3-MA) and AMP-activated protein kinase (AMPK) activator (AICAR)/inhibitor (Compound C) were used to determine the involvement of AMPK and autophagy in the regulation of lipid accumulation by empagliflozin. Ten-eleven translocation 2 (TET2) knockdown was achieved by siRNA transfection. Hepatic steatosis was evaluated by Oil Red O staining and triglyceride quantification. Immunohistochemistry, immunofluorescence, and western blot were performed to assess protein levels.

Results: Empagliflozin alleviated liver steatosis in db/db mice and reduced triglyceride content and lipid accumulation in the hepatocyte steatosis model. Empagliflozin elevated autophagy, accompanied by an increase in p-AMPK and TET2. Both 3-MA and Compound C abolished the ability of empagliflozin to induce autophagy and reduce hepatic steatosis, while these effects could be recapitulated by AICAR treatment. TET2 knockdown resulted in autophagy inhibition and lipid accumulation despite empagliflozin treatment.

Conclusion: Empagliflozin improves hepatic steatosis through the AMPK-TET2-autophagy pathway. The use of empagliflozin as a treatment for preventing and treating MAFLD in patients with T2DM warrants further study.
Epidemics Forecast From SIR-Modeling, Verification and Calculated Effects of Lockdown and Lifting of Interventions
Authors: Schlickeiser R. , Kröger M.
Published: January 2021

Due to the current COVID-19 epidemic plague hitting the worldwide population it is of utmost medical, economical and societal interest to gain reliable predictions on the temporal evolution of the spreading of the infectious diseases in human populations. Of particular interest are the daily rates and cumulative number of new infections, as they are monitored in infected societies, and the influence of non-pharmaceutical interventions due to different lockdown measures as well as their subsequent lifting on these infections. Estimating quantitatively the influence of a later lifting of the interventions on the resulting increase in the case numbers is important to discriminate this increase from the onset of a second wave. The recently discovered new analytical solutions of Susceptible-Infectious-Recovered (SIR) model allow for such forecast. In particular, it is possible to test lockdown and lifting interventions because the new solutions hold for arbitrary time dependence of the infection rate. Here we present simple analytical approximations for the rate and cumulative number of new infections.
Anomalous Behavior of Highly Active Helical Swimmers
Authors: Patil Gouri , Ghosh Ambarish
Published: January 2021

Artificially designed self-propelled objects can allow studying active matter phenomena with great detail that is not possible in natural, e.g. biological systems. Here, we show experimental results on helical shaped, magnetically actuated, reciprocal swimmers, where the degree of randomness in the reciprocal sequence plays an important role in determining their effective motility. Here, for the first time we show the results at high activity levels where the degree of randomness is further affected by the presence of the surface, which in turn results in a non-monotonic increase of motility as a function of magnetic drive. It will be interesting to extend these studies to denser systems where the swimmers can interact with each other through hydrodynamic forces.
Age-Related Pathways in Cardiac Regeneration: A Role for lncRNAs?
Authors: Santos Francisco , Correia Magda , Nóbrega-Pereira Sandrina , Bernardes de Jesus Bruno
Published: January 2021

Aging imposes a barrier for tissue regeneration. In the heart, aging leads to a severe rearrangement of the cardiac structure and function and to a subsequent increased risk of heart failure. An intricate network of distinct pathways contributes to age-related alterations during healthy heart aging and account for a higher susceptibility of heart disease. Our understanding of the systemic aging process has already led to the design of anti-aging strategies or to the adoption of protective interventions. Nevertheless, our understanding of the molecular determinants operating during cardiac aging or repair remains limited. Here, we will summarize the molecular and physiological alterations that occur during aging of the heart, highlighting the potential role for long non-coding RNAs (lncRNAs) as novel and valuable targets in cardiac regeneration/repair.
The S2–S3 Loop of Kv7.4 Channels Is Essential for Calmodulin Regulation of Channel Activation
Authors: Zhuang Wenhui , Yan Zhiqiang
Published: January 2021

Kv7.4 (KCNQ4) voltage-gated potassium channels control excitability in the inner ear and the central auditory pathway. Mutations in Kv7.4 channels result in inherited progressive deafness in humans. Calmodulin (CaM) is crucial for regulating Kv7 channels, but how CaM affects Kv7 activity has remained unclear. Here, based on electrophysiological recordings, we report that the third EF hand (EF3) of CaM controls the calcium-dependent regulation of Kv7.4 activation and that the S2–S3 loop of Kv7.4 is essential for the regulation mediated by CaM. Overexpression of the mutant CaM1234, which loses the calcium binding ability of all four EF hands, facilitates Kv7.4 activation by accelerating activation kinetics and shifting the voltage dependence of activation leftwards. The single mutant CaM3, which loses the calcium binding ability of the EF3, phenocopies facilitating effects of CaM1234 on Kv7.4 activation. Kv7.4 channels co-expressed with wild-type (WT) CaM show inhibited activation when intracellular calcium levels increase, while Kv7.4 channels co-expressed with CaM1234 or CaM3 are insensitive to calcium. Mutations C156A, C157A, C158V, R159, and R161A, which are located within the Kv7.4 S2–S3 loop, dramatically facilitate activation of Kv7.4 channels co-expressed with WT CaM but have no effect on activation of Kv7.4 channels co-expressed with CaM3, indicating that these five mutations decrease the inhibitory effect of Ca2+/CaM. The double mutation C156A/R159A decreases Ca2+/CaM binding and completely abolishes CaM-mediated calcium-dependent regulation of Kv7.4 activation. Taken together, our results provide mechanistic insights into CaM regulation of Kv7.4 activation and highlight the crucial role of the Kv7.4 S2–S3 loop in CaM regulation.
Comparison of Aerobic and Muscular Power Between Junior/U23 Slalom and Sprint Paddlers: An Analysis of International Medalists and Non-medalists
Authors: Bielik Viktor , Lendvorský Leonard , Vajda Matej , Lopata Peter , Ružbarský Pavel , Masselli dos Reis Ivan Gustavo , Messias Leonardo Henrique Dalcheco
Published: January 2021

This study aimed to compare the aerobic power (treadmill running) and muscle power (bench press and bench pull) of Junior/U23 paddlers from Slovakia who won medals in international championships with that of those who did not take the podium. Forty-three Slovak Junior/U23 paddlers (sprint = 24, medalists = 8, non-medalists = 16; slalom = 19, medalists = 11, non-medalists = 8) were tested in 2018 and 2019 after the world championships. The maximal oxygen uptake (VO2max) and the velocity at maximal oxygen uptake (vVO2max) were determined by the incremental running protocol (0% slope and 1 km⋅h–1increments every minute until volitional exhaustion). Mean maximal power from the entire concentric phase was recorded during bench press and bench pull exercises by the validated TENDO weightlifting analyzer. No interaction was obtained between medal and canoe discipline for VO2max(p= 0.069,F= 3.495), vVO2max(p= 0.552,F= 0.361) and absolute (bench press:p= 0.486,F= 0.495; bench pull:p= 0.429,F= 0.640) or relative (bench press:p= 0.767,F= 0.089; bench pull:p= 0.696,F= 0.155) mean maximal power. Conversely, a significant effect for the medal on the bench press (absolutep= 0.017,F= 6.170; relativep= 0.043,F= 4.384) and the bench pull (absolutep= 0.041,F= 4.470) mean maximal power were observed. Our study indicates the absolute mean power on the bench press as a prerequisite for success in international Junior/U23 championships of slalom and sprint canoeing. However, the mean power on bench pull seems to have a deeper influence on sprint paddlers when compared to slalom athletes. Regarding the aerobic power, the data from the treadmill testing did not reveal outcomes between medalists and non-medalists. This result can be associated with the lack of specificity of the incremental treadmill testing for canoeing, and future studies are encouraged to propose specific protocols to compare the aerobic power of medalists and non-medalists in international slalom and sprint championships.
Function Analysis of P450 and GST Genes to Imidacloprid in Aphis craccivora (Koch)
Authors: Yang Yuan-Xue , Lin Rong-Hua , Li Zhuo , Wang Ai-Yu , Xue Chao , Duan Ai-Ling , Zhao Ming , Zhang Jian-Hua
Published: January 2021

Aphis craccivora (Koch) is an economically important pest that affects legumes in worldwide. Chemical control is still the primary efficient method for A. craccivora management. However, the mechanism underlying insecticide resistance in A. craccivora has not been elucidated. A previous study observed that piperonyl butoxide (PBO) and diethyl maleate (DEM) significantly synergized imidacloprid in A. craccivora field populations, indicating that cytochrome P450 (P450) and glutathione S-transferase (GST) genes may play pivotal roles in imidacloprid resistance. In this study, 38 P450 genes and 10 GST genes were identified in A. craccivora through transcriptomic analysis. The expression levels of these P450 and GST genes were measured in susceptible (SUS) strains of A. craccivora under imidacloprid treatment with LC15, LC50, and LC85 doses. The expression levels of CYP18A1, CYP6CY21, CYP6DA1, CYP6DA2, CYP4CJ1, CYP4CJ2, and CYP380C6 were up-regulated in the three treatments. Most of these genes belong to CYP3 and CYP4 Clans. In addition, the expression levels of all P450 and GST genes in A. craccivora were also measured in the Juye (JY) and Linqing (LQ) field populations. The expression levels of CYP6DA2, CYP4CJ1, and CYP380C6 were up-regulated in the SUS strain after imidacloprid treatment at three doses, and these genes were overexpressed in the JY population. Furthermore, the sensitivity of A. craccivora to imidacloprid was significantly increased after knockdown of CYP380C6 and CYP6DA2 through RNA interference. These results may help to elucidate the mechanisms underlying of imidacloprid resistance in A. craccivora.
Contrast Tempo of Movement and Its Effect on Power Output and Bar Velocity During Resistance Exercise
Authors: Wilk Michal , Jarosz Jakub , Krzysztofik Michal , Filip-Stachnik Aleksandra , Bialas Marcin , Rzeszutko-Belzowska Agata , Zajac Adam , Stastny Petr
Published: January 2021

In this study, we examined the impact of contrast movement tempo (fast vs. slow) on power output and bar velocity during the bench press exercise. Ten healthy men (age = 26.9 ± 4.1 years; body mass = 90.5 ± 10.3 kg; bench press 1RM = 136.8 ± 27.7 kg) with significant experience in resistance training (9.4 ± 5.6 years) performed the bench press exercise under three conditions: with an explosive tempo of movement in each of three repetitions (E/E/E = explosive, explosive, explosive); with a slow tempo of movement in the first repetition and an explosive tempo in the next two repetitions (S/E/E = slow, explosive, explosive); and with a slow tempo of movement in the first two repetitions and an explosive tempo in the last repetition (S/S/E = slow, slow, explosive). The slow repetitions were performed with a 5/0/5/0 (eccentric/isometric/concentric/isometric) movement tempo, while the explosive repetitions were performed with an X/0/X/0 (X- maximal speed of movement) movement tempo. During each experimental session, the participants performed one set of three repetitions at 60%1RM. The two-way repeated measures ANOVA showed a statistically significant interaction effect for peak power output (PP; p = 0.03; η2 = 0.26) and for peak bar velocity (PV; p = 0.04; η2 = 0.24). Futhermore there was a statistically significant main effect of condition for PP (p = 0.04; η2 = 0.30) and PV (p = 0.02; η2 = 0.35). The post hoc analysis for interaction revealed that PP was significantly higher in the 2nd and 3rd repetition for E/E/E compared with the S/S/E (p < 0.01 for both) and significantly higher in the 2nd repetition for the S/E/E compared with S/S/E (p < 0.01). The post hoc analysis for interaction revealed that PV was significantly higher in the 2nd and 3rd repetition for E/E/E compared with the S/S/E (p < 0.01 for both), and significantly higher in the 2nd repetition for the S/E/E compared with the S/S/E (p < 0.01). The post hoc analysis for main effect of condition revealed that PP and PV was significantly higher for the E/E/E compared to the S/S/E (p = 0.04; p = 0.02; respectively). The main finding of this study was that different distribution of movement tempo during a set has a significant impact on power output and bar velocity in the bench press exercise at 60%1RM. However, the use of one slow repetition at the beginning of a set does not decrease the level of power output in the third repetition of that set.
The Impact of a Single Stretching Session on Running Performance and Running Economy: A Scoping Review
Authors: Konrad Andreas , Močnik Richard , Nakamura Masatoshi , Sudi Karl , Tilp Markus
Published: January 2021

One determining factor for running performance is running economy (RE), which can be quantified as the steady-state oxygen consumption at a given running speed. Stretching is frequently applied in sports practice and has been widely investigated in recent years. However, the effect of stretching on RE and performance is not clear. Thus, the purpose of this scoping review is to investigate the effects of a single bout of stretching on RE and running performance in athletes (e.g., recreational and elites) and non-athletes. The online search was performed in PubMed, Scopus, and Web of Science databases. Only studies that explored the acute effects of stretching on RE (or similar variables) and/or running performance variables with healthy and adult participants, independent of activity level, were included in this review. Eleven studies met the inclusion criteria with a total of 44 parameters (14 performance-related/30 metabolic parameters) and 111 participants. Regardless of the stretching technique, there was an improvement both in performance variables (21.4%) and metabolic variables (13.3%) following an acute bout of stretching. However, detrimental effects in performance variables (28.5%) and metabolic variables (6.6%) were also reported, though the results were influenced by the stretching duration and technique. Although it was observed that a single static stretching exercise with a duration of up to 90 s per muscle group can lead to small improvements in RE (1.0%; 95% CI: −1.04 to 2.22), negative effects were reported in running performance (−1.4%; 95% CI: −3.07 to −0.17). It was also observed that a single bout of dynamic stretching only resulted in a negligible change in RE −0.79% (95% CI: −0.95 to 4.18) but a large increase in running performance (9.8%; 95% CI: −3.28 to 16.78), with an overall stretch duration (including all muscles) between 217 and 900 s. Therefore, if stretching is applied without additional warm-up, the results suggest applying dynamic stretching (for a short overall stretching duration of ≤220 s) rather than static stretching if the goal is to increase running performance. In general, only short static stretching durations of ≤60 s per muscle–tendon unit are advisable. One study reported that less flexible runners have greater benefits from stretching than athletes with normal flexibility. In addition, it can be suggested that less flexible runners should aim for an optimum amount of flexibility, which would likely result in a more economical run.
Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement of in vitro Growth in Scrophularia kakudensis—A Potential Medicinal Plant
Authors: Manivannan Abinaya , Soundararajan Prabhakaran , Park Yoo Gyeong , Jeong Byoung Ryong
Published: January 2021

The current study has determined the effect of red and blue lights on the enhancement of growth, antioxidant property, phytochemical contents, and expression of proteins in Scrophularia kakudensis. In vitro-grown shoot tip explants of S. kakudensis were cultured on the plant growth regulator-free Murashige and Skoog (MS) medium and cultured under the conventional cool white fluorescent lamp (control), blue light-emitting diodes (LED) light, or red LED light. After 4 weeks, growth, stomatal ultrastructure, total phenols and flavonoids, activities of antioxidant enzymes, and protein expressions were determined. Interestingly, blue or red LED treatment increased the shoot length, shoot diameter, root length, and biomass on comparison with the control. In addition, the LED treatments enhanced the contents of phytochemicals in the extracts. The red LED treatment significantly elicited the accumulation of flavonoids in comparison with the control. In accordance with the secondary metabolites, the LED treatments modulated the activities of antioxidant enzymes. Moreover, the proteomic insights using two-dimensional gel electrophoresis system revealed the proteins involved in transcription and translation, carbohydrate mechanism, post-translational modification, and stress responses. Taken together, the incorporation of blue or red LED during in vitro propagation of S. kakudensis can be a beneficial way to increase the plant quality and medicinal values of S. kakudensis.
Effect of Exogenous Gibberellin, Paclobutrazol, Abscisic Acid, and Ethrel Application on Bulblet Development in Lycoris radiata
Authors: Xu Junxu , Li Qingzhu , Li Ye , Yang Liuyan , Zhang Yongchun , Cai Youming
Published: January 2021

Lycoris species have great ornamental and medicinal values; however, their low regeneration efficiency significantly restricts their commercial production. Exogenous hormone application is an effective way to promote bulblet development, but their effect on Lycoris radiata has not been verified to date. In the present study, we examined the effect of different exogenous hormones on bulblet development in L. radiata, and found that gibberellic acid (GA) significantly inhibited, whereas paclobutrazol (PBZ), abscisic acid (ABA), and ethrel promoted bulblet development, especially PBZ, a GA biosynthesis inhibitor. Furthermore, GA reduced endogenous cytokinin (CK) content, as well as the activities of carbohydrate metabolism enzymes, including sucrose synthase (SUS) and glucose-1-phosphate adenylyltransferase (AGPase), by downregulating the expression levels of LrSUS1, LrSUS2, and genes encoding AGPase large and small subunits. This resulted in the decrease in carbohydrate accumulation in the bulblets, thus hindering their development. PBZ had the opposite effect to GA on carbohydrate metabolism; it decreased endogenous GA15 and GA24, thereby promoting bulblet development. ABA promoted endogenous auxin content and the activities of starch synthesis enzymes, especially soluble starch synthase (SSS) and granule-bound SS (GBSS), through the up-regulation of the expression levels of LrSS1, LrSS2, and LrGBSS1 genes, which could also result in the accumulation of carbohydrates in the bulblets and promote their development. In addition, ethrel application partly promoted bulblet development by promoting endogenous CK content. Although the accumulation of carbohydrates and the activity of starch enzymes were increased by ethrel treatment, we hypothesized that the effect of ethrel on regulating carbohydrate metabolism may be indirect. Our results could provide a basis for improving the propagation efficiency of L. radiata for production, as well as propose some directions for future research.
Microcutting Redox Profile and Anatomy in Eucalyptus spp. With Distinct Adventitious Rooting Competence
Authors: Vilasboa Johnatan , da Costa Cibele Tesser , Ransan Leonardo Girardi , Mariath Jorge Ernesto de Araújo , Fett-Neto Arthur Germano
Published: January 2021

Adventitious root (AR) development takes place in an intricate cellular environment. Reactive oxygen species (ROS) and antioxidant defenses, triggered by wounding in cuttings, can modulate this process. A comparative assessment of biochemical and anatomical parameters at critical rooting stages in hard- (Eucalyptus globulus Labill.) and easy- (Eucalyptus grandis W.Hill ex Maiden) to-root species was carried out. Microcuttings from seedlings were inoculated in auxin-free AR induction medium and, after 96 h, transferred to AR formation medium for a period of 24 h. Samples were collected upon excision (Texc) and at the 5th day post excision (Tform). Delayed xylem development, with less lignification, was recorded in E. globulus, when compared to E. grandis, suggesting lower activity of the cambium layer, an important site for AR development. Superoxide was more densely present around the vascular cylinder at both sampled times, and in greater quantity in E. globulus than E. grandis, declining with time in the former. Hydrogen peroxide was localized primarily along cell walls, more intensely in the primary xylem and phloem, and increased significantly at Tform in E. globulus. Ascorbate peroxidase (APX), superoxide dismutase (SOD), and catalase (CAT) activities were generally higher in E. grandis and varied as a function of time in E. globulus. Soluble guaiacol peroxidase (GPRX) activity increased from Texc to Tform in both species, whereas cell wall-bound GPRX activity increased with time in E. grandis, surpassing E. globulus. Flavonoid content increased with time in E. grandis and was higher than E. globulus at Tform. Principal component analysis showed that species- and time-derived differences contributed to almost 80% of the variance. Overall, data indicate that E. grandis shows higher cambium activity and tighter modulation of redox conditions than E. globulus. These features may influence ROS-based signaling and phytohormone homeostasis of cuttings, thereby impacting on AR development. Besides being players in the realm of AR developmental differences, the specific features herein identified could become potential tools for early clone selection and AR modulation aiming at improved clonal propagation of this forest crop.
Sharing Data – Not With Us! Distrust as Decisive Obstacle for Public Authorities to Benefit From Sharing Economy
Authors: Nienaber Ann-Marie Ingrid , Woodcock Andree , Liotopoulos Fotis K.
Published: January 2021

Future mobility planning to cope with ongoing environmental challenges such as air pollution has to be anchored in the work of every public authority worldwide. One recent trend that could support public authorities to meet the European Union’s sustainability targets is the creation and sharing of transport and mobility “big” data between public authorities via tools such as crowdsourcing. While the benefits of the use of big data to increase public authorities’ efficiency and effectivity and their citizens’ lives is well understood, examples from the public sector that highlight public authorities’ engagement in such sharing activities is still missing. To date relevant literature has highlighted issues around the capacity of public authorities that hinder shared activities. In this paper we want to raise distrust as a key reason for lack of engagement. Based on comprehensive data collected over the period of 4 years via several workshops and semi-structured interviews with seven public authorities in Europe, we are able to demonstrate that a major obstacle for not providing and sharing data via crowdsourcing for mutual benefit lies primarily in the hands of the public authority’s servants of the middle and high-level management. Our results show firstly, that distrust may emerge toward different referents such as the community, particular individuals, or the technology itself and thus, managerial implications have to be very specific to overcome distrust. Secondly, we show how distrust may spread from one referent to another through negative reciprocity and which, if unchecked may lead to an all-encompassing state that affects the whole sharing economy framework and inhibits potential benefits.
Effects of Dynamic Resilience on the Reactivity of Vagally Mediated Heart Rate Variability
Authors: Crameri Luke , Hettiarachchi Imali T. , Hanoun Samer
Published: January 2021

Dynamic resilience is a novel concept that aims to quantify how individuals are coping while operating in dynamic and complex task environments. A recently developed dynamic resilience measure, derived through autoregressive modeling, offers an avenue toward dynamic resilience classification that may yield valuable information about working personnel for industries such as defense and elite sport. However, this measure classifies dynamic resilience based upon in-task performance rather than self-regulating cognitive structures; thereby, lacking any supported self-regulating cognitive links to the dynamic resilience framework. Vagally mediated heart rate variability (vmHRV) parameters are potential physiological measures that may offer an opportunity to link self-regulating cognitive structures to dynamic resilience given their supported connection to the self-regulation of stress. This study examines if dynamic resilience classifications reveal significant differences in vagal reactivity between higher, moderate and lower dynamic resilience groups, as participants engage in a dynamic, decision-making task. An amended Three Rs paradigm was implemented that examined vagal reactivity across six concurrent vmHRV reactivity segments consisting of lower and higher task load. Overall, the results supported significant differences between higher and moderate dynamic resilience groups' vagal reactivity but rejected significant differences between the lower dynamic resilience group. Additionally, differences in vagal reactivity across vmHRV reactivity segments within an amended Three Rs paradigm were partially supported. Together, these findings offer support toward linking dynamic resilience to temporal self-regulating cognitive structures that play a role in mediating physiological adaptations during task engagement.