bluuebunny/crossref_metadata_2025 huggingface dataset:
An Emerging Syndemic of Smoking and Cardiopulmonary Diseases in People Living with HIV in Africa
Authors: Peprah Emmanuel , Armstrong-Hough Mari , Cook Stephanie H. , Mukasa Barbara , Taylor Jacquelyn Y. , Xu Huichun , Chang Linda , Gyamfi Joyce , Ryan Nessa , Ojo Temitope , Snyder Anya , Iwelunmor Juliet , Ezechi Oliver , Iyegbe Conrad , O’Reilly Paul , Pascal Kengne Andre
Published: March 2021

Background: African countries have the highest number of people living with HIV (PWH). The continent is home to 12% of the global population, but accounts for 71% of PWH globally. Antiretroviral therapy has played an important role in the reduction of the morbidity and mortality rates for HIV, which necessitates increased surveillance of the threats from pernicious risks to which PWH who live longer remain exposed. This includes cardiopulmonary comorbidities, which pose significant public health and economic challenges. A significant contributor to the cardiopulmonary comorbidities is tobacco smoking. Indeed, globally, PWH have a 2–4-fold higher utilization of tobacco compared to the general population, leading to endothelial dysfunction and atherogenesis that result in cardiopulmonary diseases, such as chronic obstructive pulmonary disease and coronary artery disease. In the context of PWH, we discuss (1) the current trends in cigarette smoking and (2) the lack of geographically relevant data on the cardiopulmonary conditions associated with smoking; we then review (3) the current evidence on chronic inflammation induced by smoking and the potential pathways for cardiopulmonary disease and (4) the multifactorial nature of the syndemic of smoking, HIV, and cardiopulmonary diseases. This commentary calls for a major, multi-setting cohort study using a syndemics framework to assess cardiopulmonary disease outcomes among PWH who smoke. Conclusion: We call for a parallel program of implementation research to promote the adoption of evidence-based interventions, which could improve health outcomes for PWH with cardiopulmonary diseases and address the health inequities experienced by PWH in African countries.
Salivary Characteristics, Individual Casual Parameters, and Their Relationships with the Significant Caries Index among Korean Children Aged 12 Years
Authors: Kim Jae-Hwan , Kim Mi-Ah , Chae Yong Kwon , Nam Ok Hyung
Published: March 2021

This study aimed to investigate the salivary characteristics and individual daily living patterns in Korean children aged 12 years and evaluate their relationships according to the significant caries (SiC) index. The study sample consisted of 52 healthy Korean children. The subjects were allocated into a low caries-affected (low CA) group and a high caries-affected (high CA) group, according to the SiC index. Children underwent a standardized oral examination, and parents completed the questionnaires. Stimulated salivary samples were collected to evaluate the salivary pH, salivary flow rate, and salivary levels of Mutans streptococci (MS) and Lactobacilli (LB). The low CA group did not significantly differ from the high CA group for salivary flow rate and salivary pH. However, there were significant differences in salivary MS levels between the two groups (p < 0.05). Among the individual casual parameters, the prevalence of a sugar-associated primary energy source between meals was significantly higher in the high CA group than in the low CA group (p < 0.05). Within the limitations of this study, different levels of salivary MS and the consumption of different foods were observed in the low CA and high CA groups. The implications of these findings should be considered for caries susceptibility.
Concentration and Spatial Distribution of Potentially Toxic Elements in Surface Soil of a Peak-Cluster Depression, Babao Town, Yunnan Province, China
Authors: Tian Hongyu , Zhang Cheng , Qi Shihua , Kong Xiangsheng , Yue Xiangfei
Published: March 2021

Potentially toxic elements (PTEs) in Chinese agricultural soils, including those in some heritage protection zones, are serious and threaten food safety. Many scientists think that these PTEs may come from parent rock. Hence, at a karst rice-growing agricultural heritage area, Babao town, Guangnan County, Yunnan Province, China, the concentrations of eight PTEs (As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn) were determined in 148 surface soil, 25 rock, and 52 rice grain samples. A principal component analysis (PCA) and hierarchical cluster analysis were used to divide the surface soil into groups, and inverse distance weighting (IDW) was used to analyze the spatial distribution of PTEs. Soil pollution was assessed with the geoaccumulation index (Igeo). The results show that Cd, Hg, Zn, and Cr were polluting the soil (average Igeo > 0). The highest concentration of PTEs was distributed in the southwest of Babao town in the carbonate rock area, which had the highest pH and soil total organic carbon (Corg), Mn, and TFe2O3 contents. PCA biplots of soil samples showed that the carbonate rock area was associated with the most species of PTEs in the study area including Pb, Cd, Hg, As, and Zn. The clastic rock area was associated with Cu and Ni, and the lime and cement plants were associated with CaO, pH, Corg, TC, and aggravated PTE pollution around factories. In high-level PTE areas, rice was planted. Two out of 52 rice grain samples contained Cd and 4 out of 52 rice grain samples had Cr concentrations above the Chinese food safety standard pollutant limit (Cd 0.2 mg/kg; Cr 1 mg/kg). Therefore, the PTEs from parent rocks are already threatening rice safety. The government should therefore plan rice cultivation areas accordingly.
Effects of Aquatic Therapy for Children with Autism Spectrum Disorder on Social Competence and Quality of Life: A Mixed Methods Study
Authors: Güeita-Rodríguez Javier , Ogonowska-Slodownik Anna , Morgulec-Adamowicz Natalia , Martín-Prades Mar Lledó , Cuenca-Zaldívar Juan Nicolás , Palacios-Ceña Domingo
Published: March 2021

Autism Spectrum Disorder (ASD) is a constellation of social deficits and repetitive sensory-motor behaviours. Aquatic therapy (AT) may be effective in improving the social interactions and behaviours in children with ASD. The objective of this study was to evaluate the effects of an AT program on social competence and quality of life and to understand participant’s experiences related to the intervention by obtaining qualitative data. A mixed methods intervention study was conducted among 6 children with ASD and their parents, with two research phases in a concurrent embedded design (an aquatic intervention as the quantitative design and a qualitative design in second step). The intervention and qualitative design followed international guidelines and were integrated into the method and reporting subheadings. Significant improvement was observed in the physical competence (p = 0.026) and important improvements in school functioning and aquatic skills, with no adverse events. Qualitative findings described: the meaning of AT intervention, patterns of behaviour and activities changes, social communication and social interaction. The aquatic intervention showed positive results for the social and physical competence, with elements of discordance, expansion, and confirmation between quantitative and qualitative results.
Prenatal Exposure to Alcohol, Tobacco, and Coffee: Associated Congenital Complications and Adverse Birth Outcomes
Authors: Oh Sarah Soyeon , Park Sunwha , You Young-Ah , Jee Yongho , Ansari AbuZar , Kim Soo Min , Lee Gain , Kim Young Ju
Published: March 2021

A few studies to date have examined the association between prenatal exposure to alcohol, tobacco, and coffee, and congenital complications/adverse birth outcomes among South Korean populations. Thus, this study analyzed the data of 1675 Korean women with birth experience within the last 3 years for pregnancy-related health and nutritional behaviors and relative outcomes. During their pregnancies, 11.58% of the study population consumed alcohol at least once, 1.43% drank throughout all three trimesters, 1.13% smoked, 25.43% were exposed to secondhand smoking, and 28.18% consumed 3 coffees or more every day. Prenatal alcohol exposure was associated with 11.24 times increased risk of birth defects/disabilities [Odds Ratio (OR): 11.24, 95% Confidence Interval (CI) 1.07–117.86] and 10.66 times increased risk of inherited metabolic diseases (OR: 10.66, 95% CI: 1.08–104.82). Prenatal secondhand smoke exposure (OR: 1.62, 95% CI: 1.01–2.62) and coffee consumption (OR: 1.92, 95% CI: 1.22–3.03) was associated with increased risk of low birth weight. Such results were in alignment with that of previous studies and confirmed that prenatal alcohol, tobacco, and coffee exposure can have detrimental neonatal and maternal consequences.
The Neighborhood Contagion Focus as a Spatial Unit for Diagnosis and Epidemiological Action against COVID-19 Contagion in Urban Spaces: A Methodological Proposal for Its Detection and Delimitation
Authors: Perles María-Jesús , Sortino Juan F. , Mérida Matías F.
Published: March 2021

The concept of neighborhood contagion focus is defined and justified as a basic spatial unit for epidemiological diagnosis and action, and a specific methodological procedure is provided to detect and map focuses and micro-focuses of contagion without using regular or artificial spatial units. The starting hypothesis is that the contagion in urban spaces manifests unevenly in the form of clusters of cases that are generated and developed by neighborhood contagion. Methodologically, the spatial distribution of those infected in the study area, the city of Málaga (Spain), is firstly analyzed from the disaggregated and anonymous address information. After defining the concept of neighborhood contagion focus and justifying its morphological parameters, a method to detect and map neighborhood contagion focus in urban settings is proposed and applied to the study case. As the main results, the existence of focuses and micro-focuses in the spatial pattern of contagion is verified. Focuses are considered as an ideal spatial analysis unit, and the advantages and potentialities of the use of mapping focus as a useful tool for health and territorial management in different phases of the epidemic are shown.
Effect of Progressive Step Marching Exercise on Balance Ability in the Elderly: A Cluster Randomized Clinical Trial
Authors: Sitthiracha Pairaya , Eungpinichpong Wichai , Chatchawan Uraiwan
Published: March 2021

Exercise may reduce the risk of falls in the elderly. The objective of the study was to determine the effect of a progressive step marching exercise (PSME) program on balance ability, lower limb muscle strength, aerobic capacity, quality of life, and fear of falling in the elderly. A cluster randomized controlled trial was selected where 30 elderly participants (aged 69 ± 3 years) from a community were supervised while performing a PSME program and 30 (aged 70 ± 3 years) from another community were assigned to a control group. All participants in both groups underwent timed up and go test, one leg standing test, five time sit to stand test, two minutes step test, World Health Organization Quality of Life-Thai version, and fall efficacy scale Thai version at baseline, after exercise at 4 and 8 weeks. The PSME group underwent the program for 8 weeks while those in the control group were instructed to continue their normal activity. After training, the PSME showed significant improvement (p < 0.05) in all parameters except one leg standing and two minutes step test when compared to the control group (p < 0.05). In conclusion, the PSME program could improve balance ability, lower limb muscle strength, quality of life, and fear of falling in the elderly.
Testing the Functional Model of Bone Development: Direct and Mediating Role of Muscle Strength on Bone Properties in Growing Youth
Authors: Ludwa Izabella A. , Mongeon Kevin , Sanderson Malcolm , Gracia Marco Luis , Klentrou Panagiota
Published: March 2021

This study examines the functional model of bone development in peri-pubertal boys and girls. Specifically, we implemented a mixed-longitudinal design and hierarchical structural models to provide experimental evidence in support of the conceptual functional model of bone development, postulating that the primary mechanical stimulus of bone strength development is muscle force. To this end, we measured radial and tibial bone properties (speed of sound, SOS), isometric grip and knee extensors strength, bone resorption (urinary NTX concentration), body mass index (BMI), somatic maturity (years from peak height velocity) and skeletal maturity (bone age) in 180 children aged 8–16 years. Measurements were repeated 2–4 times over a period of 3 years. The multilevel structural equation modeling of 406 participant-session observations revealed similar results for radial and tibial SOS. Muscle strength (i.e., grip strength for the radial and knee extension for tibial model) and NTX have a significant direct effect on bone SOS (β = 0.29 and −0.18, respectively). Somatic maturity had a direct impact on muscle strength (β = 0.24) and both a direct and indirect effect on bone SOS (total effect, β = 0.30). Physical activity and BMI also had a significant direct impact on bone properties, (β = 0.06 and −0.18, respectively), and an additional significant indirect effect through muscle strength (β = 0.01 and 0.05, respectively) with small differences per bone site and sex. Muscle strength fully mediated the impact of bone age (β = 0.14) while there was no significant effect of energy intake on either muscle strength or bone SOS. In conclusion, our results support the functional model of bone development in that muscle strength and bone metabolism directly affect bone development while the contribution of maturity, physical activity, and other modulators such as BMI, on bone development is additionally modulated through their effect on muscle strength.
An Orthodontic Approach for Garre’s Sclerosing Osteomyelitis of the Mandible
Authors: Barbur Ioan , Bran Simion , Baciut Mihaela , Armencea Gabriel , Aghiorghiesei Alexandra Iulia , Suciu Tudor-Sergiu , Barbur Adina Maria , Opris Horia , Baciut Grigore , Dinu Cristian
Published: March 2021

The nonsuppurative osteomyelitis of the mandible is a rare condition that can occur in children due to low-grade inflammatory processes, dental cavities, periodontal lesions as well as the eruption process of the teeth. We submit a case report involving the orthodontic management of a 9-year-old female patient who presented in our service in the mixed dentition period with diagnosed Garre’s sclerosing osteomyelitis of the entire mandibular body. After a full work-up, the following symptoms and signs were noted: bilateral temporomandibular joint (TMJ) pain, loss of the leeway space, anterior open bite, distalization of the secondary maxillary right canine, nail biting and tongue thrust. Our orthodontic objectives were to relieve the TMJ pain, limit the eruption process of the teeth and to diminish the evolution of the osteomyelitis, reduce the growth of the inferior lower third of the face and to prevent further invasive treatment of the patient. In the first phase of treatment, we established a centric relationship using an orthopedic appliance (occlusal splint) and physiotherapy to deprogram the muscles and the TMJ. Throughout the second phase of treatment, we used orthopedic appliances to inhibit the overeruption of the secondary molars. After another year of treatment, the osteomyelitis lesions were under control with the permanent teeth in final position, good facial esthetic and as a functional result, no root resorption. We can conclude that by using low physiological forces to direct and control the growth pattern, good results could be obtained in stabilizing and controlling the sclerosing osteomyelitis of the mandible.
Predicting Proteolysis in Complex Proteomes Using Deep Learning
Authors: Ozols Matiss , Eckersley Alexander , Platt Christopher I. , Stewart-McGuinness Callum , Hibbert Sarah A. , Revote Jerico , Li Fuyi , Griffiths Christopher E. M. , Watson Rachel E. B. , Song Jiangning , Bell Mike , Sherratt Michael J.
Published: March 2021

Both protease- and reactive oxygen species (ROS)-mediated proteolysis are thought to be key effectors of tissue remodeling. We have previously shown that comparison of amino acid composition can predict the differential susceptibilities of proteins to photo-oxidation. However, predicting protein susceptibility to endogenous proteases remains challenging. Here, we aim to develop bioinformatics tools to (i) predict cleavage site locations (and hence putative protein susceptibilities) and (ii) compare the predicted vulnerabilities of skin proteins to protease- and ROS-mediated proteolysis. The first goal of this study was to experimentally evaluate the ability of existing protease cleavage site prediction models (PROSPER and DeepCleave) to identify experimentally determined MMP9 cleavage sites in two purified proteins and in a complex human dermal fibroblast-derived extracellular matrix (ECM) proteome. We subsequently developed deep bidirectional recurrent neural network (BRNN) models to predict cleavage sites for 14 tissue proteases. The predictions of the new models were tested against experimental datasets and combined with amino acid composition analysis (to predict ultraviolet radiation (UVR)/ROS susceptibility) in a new web app: the Manchester proteome susceptibility calculator (MPSC). The BRNN models performed better in predicting cleavage sites in native dermal ECM proteins than existing models (DeepCleave and PROSPER), and application of MPSC to the skin proteome suggests that: compared with the elastic fiber network, fibrillar collagens may be susceptible primarily to protease-mediated proteolysis. We also identify additional putative targets of oxidative damage (dermatopontin, fibulins and defensins) and protease action (laminins and nidogen). MPSC has the potential to identify potential targets of proteolysis in disparate tissues and disease states.
Microglia Development and Maturation and Its Implications for Induction of Microglia-Like Cells from Human iPSCs
Authors: Wurm Johannes , Konttinen Henna , Andressen Christian , Malm Tarja , Spittau Björn
Published: March 2021

Microglia are resident immune cells of the central nervous system and play critical roles during the development, homeostasis, and pathologies of the brain. Originated from yolk sac erythromyeloid progenitors, microglia immigrate into the embryonic brain parenchyma to undergo final postnatal differentiation and maturation driven by distinct chemokines, cytokines, and growth factors. Among them, TGFβ1 is an important regulator of microglial functions, mediating homeostasis, anti-inflammation, and triggering the expression of microglial homeostatic signature genes. Since microglia studies are mainly based on rodent cells and the isolation of homeostatic microglia from human tissue is challenging, human-induced pluripotent stem cells have been successfully differentiated into microglia-like cells recently. However, employed differentiation protocols strongly vary regarding used cytokines and growth factors, culture conditions, time span, and cell yield. Moreover, the incomplete differentiation of human microglia can hamper the similarity to primary human microglia and dramatically influence the outcome of follow-up studies with these differentiated cells. This review summarizes the current knowledge of the molecular mechanisms driving rodent microglia differentiation in vivo, further compares published differentiation protocols, and highlights the potential of TGFβ as an essential maturation factor.
Role of the Innate Immunity Signaling Pathway in the Pathogenesis of Sjögren’s Syndrome
Authors: Shimizu Toshimasa , Nakamura Hideki , Kawakami Atsushi
Published: March 2021

Sjögren’s syndrome (SS) is a systemic autoimmune disease characterized by chronic inflammation of the salivary and lacrimal glands and extra-glandular lesions. Adaptive immune response including T- and B-cell activation contributes to the development of SS. However, its pathogenesis has not yet been elucidated. In addition, several patients with SS present with the type I interferon (IFN) signature, which is the upregulation of the IFN-stimulated genes induced by type I IFN. Thus, innate immune responses including type I IFN activity are associated with SS pathogenesis. Recent studies have revealed the presence of activation pattern recognition receptors (PRRs) including Toll-like receptors, RNA sensor retinoic acid-inducible gene I and melanoma differentiation-associated gene 5, and inflammasomes in infiltrating and epithelial cells of the salivary glands among patients with SS. In addition, the activation of PRRs via the downstream pathway such as the type I IFN signature and nuclear factor kappa B can directly cause organ inflammation, and it is correlated with the activation of adaptive immune responses. Therefore, this study assessed the role of the innate immune signal pathway in the development of inflammation and immune abnormalities in SS.
O-1602 Promotes Hepatic Steatosis through GPR55 and PI3 Kinase/Akt/SREBP-1c Signaling in Mice
Authors: Kang Saeromi , Lee Ae-Yeon , Park So-Young , Liu Kwang-Hyeon , Im Dong-Soon
Published: March 2021

Non-alcoholic fatty liver disease is recognized as the leading cause of chronic liver disease. Overnutrition and obesity are associated with hepatic steatosis. G protein-coupled receptor 55 (GPR55) has not been extensively studied in hepatic steatosis, although its endogenous ligands have been implicated in liver disease progression. Therefore, the functions of GPR55 were investigated in Hep3B human hepatoma cells and mice fed high-fat diets. O-1602, the most potent agonist of GPR55, induced lipid accumulation in hepatocytes, which was reversed by treatment with CID16020046, an antagonist of GPR55. O-1602 also induced intracellular calcium rise in Hep3B cells in a GPR55-independent manner. O-1602-induced lipid accumulation was dependent on the PI3 kinase/Akt/SREBP-1c signaling cascade. Furthermore, we found increased levels of lysophosphatidylinositol species of 16:0, 18:0, 18:1, 18:2, 20:1, and 20:2 in the livers of mice fed a high-fat diet for 4 weeks. One-week treatment with CID16020046 suppressed high-fat diet-induced lipid accumulation and O-1602-induced increase of serum triglyceride levels in vivo. Therefore, the present data suggest the pro-steatotic function of GPR55 signaling in hepatocytes and provide a potential therapeutic target for non-alcoholic fatty liver disease.
Glucocorticoid Receptor Antagonist Mifepristone Does Not Alter Innate Anxiety-Like Behavior in Genetically-Selected Marchigian Sardinian (msP) Rats
Authors: Vozella Valentina , Cruz Bryan , Natividad Luis A. , Benvenuti Federica , Cannella Nazzareno , Edwards Scott , Zorrilla Eric P. , Ciccocioppo Roberto , Roberto Marisa
Published: March 2021

Marchigian Sardinian alcohol-preferring (msP) rats serve as a unique model of heightened alcohol preference and anxiety disorders. Their innate enhanced stress and poor stress-coping strategies are driven by a genetic polymorphism of the corticotropin-releasing factor receptor 1 (CRF1) in brain areas involved in glucocorticoid signaling. The activation of glucocorticoid receptors (GRs) regulates the stress response, making GRs a candidate target to treat stress and anxiety. Here, we examined whether mifepristone, a GR antagonist known to reduce alcohol drinking in dependent rats, decreases innate symptoms of anxiety in msPs. Male and female msPs were compared to non-selected Wistar counterparts across three separate behavioral tests. We assessed anxiety-like behavior via the novelty-induced hypophagia (NIH) assay. Since sleep disturbances and hyperarousal are common features of stress-related disorders, we measured sleeping patterns using the comprehensive lab monitoring system (CLAMS) and stress sensitivity using acoustic startle measures. Rats received an acute administration of vehicle or mifepristone (60 mg/kg) 90 min prior to testing on NIH, acoustic startle response, and CLAMS. Our results revealed that both male and female msPs display greater anxiety-like behaviors as well as enhanced acoustic startle responses compared to Wistar counterparts. Male msPs also displayed reduced sleeping bout duration versus Wistars, and female msPs displayed greater acoustic startle responses versus male msPs. Importantly, the enhanced anxiety-like behavior and startle responses were not reduced by mifepristone. Together, these findings suggest that increased expression of stress-related behaviors in msPs are not solely mediated by acute activation of GRs.
Novel Ferrocene Derivatives Induce G0/G1 Cell Cycle Arrest and Apoptosis through the Mitochondrial Pathway in Human Hepatocellular Carcinoma
Authors: Zheng Jianrong , Zeng Liao , Tang Mingqing , Lin Hongjun , Pi Chao , Xu Ruian , Cui Xiuling
Published: March 2021

In this study, detailed information on hepatocellular carcinoma (HCC) cells (HepG-2, SMMC-7721, and HuH-7) and normal human liver cell L02 treated by ferrocene derivatives (compounds 1, 2 and 3) is provided. The cell viability assay showed that compound 1 presented the most potent and selective anti-HCC activity. Further mechanism study indicated that the proliferation inhibition effect of compound 1 was associated with the cycle arrest at the G0/G1 phase and downregulation of cyclin D1/CDK4. Moreover, compound 1 could induce apoptosis in HCC cells by loss of mitochondrial membrane potential (ΔΨm), accumulation of reactive oxygen species (ROS), decrease in Bcl-2, increase in BAX and Bad, translocation of Cytochrome c, activation of Caspase-9, -3, and cleavage of PARP. These results indicated that compound 1 would be a promising candidate against HCC through G0/G1 cell cycle arrest-related proliferation inhibition and mitochondrial pathway-dependent apoptosis.
Influence of Complexation of Thiosemicarbazone Derivatives with Cu (II) Ions on Their Antitumor Activity against Melanoma Cells
Authors: Pitucha Monika , Korga-Plewko Agnieszka , Czylkowska Agnieszka , Rogalewicz Bartłomiej , Drozd Monika , Iwan Magdalena , Kubik Joanna , Humeniuk Ewelina , Adamczuk Grzegorz , Karczmarzyk Zbigniew , Fornal Emilia , Wysocki Waldemar , Bartnik Paulina
Published: March 2021

A series of thiosemicarbazone derivatives was prepared and their anti-tumor activity in vitro was tested. The X-ray investigation performed for compounds T2, T3 and T5 confirmed the synthesis pathway and assumed molecular structures of analyzed thiosemicarbazones. The conformational preferences of the thiosemicarbazone system were characterized using theoretical calculations by AM1 method. Selected compounds were converted into complexes of Cu (II) ions. The effect of complexing on anti-tumor activity has been investigated. The copper(II) complexes, with Schiff bases T1, T10, T12, T13, and T16 have been synthesized and characterized by chemical and elemental analysis, FTIR spectroscopy and TGA method. Thermal properties of coordination compounds were studied using TG-DTG techniques under dry air atmosphere. G361, A375, and SK-MEL-28 human melanoma cells and BJ human normal fibroblast cells were treated with tested compounds and their cytotoxicity was evaluated with MTT test. The compounds with the most promising anti-tumour activity were then selected and their cytotoxicity was verified with cell cycle analysis and apoptosis/necrosis detection. Additionally, DNA damages in the form of a basic sites presence and the expression of oxidative stress and DNA damage response genes were evaluated. The obtained results indicate that complexation of thiosemicarbazone derivatives with Cu (II) ions improves their antitumor activity against melanoma cells. The observed cytotoxic effect is associated with DNA damage and G2/M phase of cell cycle arrest as well as disorders of the antioxidant enzymes expression.
Understanding the Potential Role of Sirtuin 2 on Aging: Consequences of SIRT2.3 Overexpression in Senescence
Authors: Sola-Sevilla Noemi , Ricobaraza Ana , Hernandez-Alcoceba Ruben , Aymerich Maria S. , Tordera Rosa M. , Puerta Elena
Published: March 2021

Sirtuin 2 (SIRT2) has been associated to aging and age-related pathologies. Specifically, an age-dependent accumulation of isoform 3 of SIRT2 in the CNS has been demonstrated; however, no study has addressed the behavioral or molecular consequences that this could have on aging. In the present study, we have designed an adeno-associated virus vector (AAV-CAG-Sirt2.3-eGFP) for the overexpression of SIRT2.3 in the hippocampus of 2 month-old SAMR1 and SAMP8 mice. Our results show that the specific overexpression of this isoform does not induce significant behavioral or molecular effects at short or long term in the control strain. Only a tendency towards a worsening in the performance in acquisition phase of the Morris Water Maze was found in SAMP8 mice, together with a significant increase in the pro-inflammatory cytokine Il-1β. These results suggest that the age-related increase of SIRT2.3 found in the brain is not responsible for induction or prevention of senescence. Nevertheless, in combination with other risk factors, it could contribute to the progression of age-related processes. Understanding the specific role of SIRT2 on aging and the underlying molecular mechanisms is essential to design new and more successful therapies for the treatment of age-related diseases.
Characterization of the Resistance to Powdery Mildew and Leaf Rust Carried by the Bread Wheat Cultivar Victo
Authors: Desiderio Francesca , Bourras Salim , Mazzucotelli Elisabetta , Rubiales Diego , Keller Beat , Cattivelli Luigi , Valè Giampiero
Published: March 2021

Leaf rust and powdery mildew are two important foliar diseases in wheat. A recombinant inbred line (RIL) population, obtained by crossing two bread wheat cultivars (‘Victo’ and ‘Spada’), was evaluated for resistance to the two pathogens at seedling stage. Upon developing a genetic map of 8726 SNP loci, linkage analysis identified three resistance Quantitative Trait Loci (QTLs), with ‘Victo’ contributing the resistant alleles to all loci. One major QTL (QPm.gb-7A) was detected in response to Blumeria graminis on chromosome 7A, which explained 90% of phenotypic variation (PV). The co-positional relationship with known powdery mildew (Pm) resistance loci suggested that a new source of resistance was identified in T. aestivum. Two QTLs were detected in response to Puccinia triticina: a major gene on chromosome 5D (QLr.gb-5D), explaining a total PV of about 59%, and a minor QTL on chromosome 2B (QLr.gb-2B). A positional relationship was observed between the QLr.gb-5D with the known Lr1 gene, but polymorphisms were found between the cloned Lr1 and the corresponding ‘Victo’ allele, suggesting that QLr.gb-5D could represent a new functional Lr1 allele. Lastly, upon anchoring the QTL on the T. aestivum reference genome, candidate genes were hypothesized on the basis of gene annotation and in silico gene expression analysis.
Lysin Motif (LysM) Proteins: Interlinking Manipulation of Plant Immunity and Fungi
Authors: Hu Shu-Ping , Li Jun-Jiao , Dhar Nikhilesh , Li Jun-Peng , Chen Jie-Yin , Jian Wei , Dai Xiao-Feng , Yang Xing-Yong
Published: March 2021

The proteins with lysin motif (LysM) are carbohydrate-binding protein modules that play a critical role in the host-pathogen interactions. The plant LysM proteins mostly function as pattern recognition receptors (PRRs) that sense chitin to induce the plant’s immunity. In contrast, fungal LysM blocks chitin sensing or signaling to inhibit chitin-induced host immunity. In this review, we provide historical perspectives on plant and fungal LysMs to demonstrate how these proteins are involved in the regulation of plant’s immune response by microbes. Plants employ LysM proteins to recognize fungal chitins that are then degraded by plant chitinases to induce immunity. In contrast, fungal pathogens recruit LysM proteins to protect their cell wall from hydrolysis by plant chitinase to prevent activation of chitin-induced immunity. Uncovering this coevolutionary arms race in which LysM plays a pivotal role in manipulating facilitates a greater understanding of the mechanisms governing plant-fungus interactions.
De novo Neurosteroidogenesis in Human Microglia: Involvement of the 18 kDa Translocator Protein
Authors: Germelli Lorenzo , Da Pozzo Eleonora , Giacomelli Chiara , Tremolanti Chiara , Marchetti Laura , Wetzel Christian H. , Barresi Elisabetta , Taliani Sabrina , Da Settimo Federico , Martini Claudia , Costa Barbara
Published: March 2021

Neuroactive steroids are potent modulators of microglial functions and are capable of counteracting their excessive reactivity. This action has mainly been ascribed to neuroactive steroids released from other sources, as microglia have been defined unable to produce neurosteroids de novo. Unexpectedly, immortalized murine microglia recently exhibited this de novo biosynthesis; herein, de novo neurosteroidogenesis was characterized in immortalized human microglia. The results demonstrated that C20 and HMC3 microglial cells constitutively express members of the neurosteroidogenesis multiprotein machinery—in particular, the transduceosome members StAR and TSPO, and the enzyme CYP11A1. Moreover, both cell lines produce pregnenolone and transcriptionally express the enzymes involved in neurosteroidogenesis. The high TSPO expression levels observed in microglia prompted us to assess its role in de novo neurosteroidogenesis. TSPO siRNA and TSPO synthetic ligand treatments were used to reduce and prompt TSPO function, respectively. The TSPO expression downregulation compromised the de novo neurosteroidogenesis and led to an increase in StAR expression, probably as a compensatory mechanism. The pharmacological TSPO stimulation the de novo neurosteroidogenesis improved in turn the neurosteroid-mediated release of Brain-Derived Neurotrophic Factor. In conclusion, these results demonstrated that de novo neurosteroidogenesis occurs in human microglia, unravelling a new mechanism potentially useful for future therapeutic purposes.
Cold Atmospheric Plasma Modification of Amyloid β
Authors: Yagi-Utsumi Maho , Tanaka Tomohiro , Otsubo Yoko , Yamashita Akira , Yoshimura Shinji , Nishida Motohiro , Kato Koichi
Published: March 2021

Cold atmospheric plasma (CAP) has attracted much attention in the fields of biotechnology and medicine owing to its potential utility in clinical applications. Recently accumulating evidence has demonstrated that CAP influences protein structures. However, there remain open questions regarding the molecular mechanisms behind the CAP-induced structural perturbations of biomacromolecules. Here, we investigated the potential effects of CAP irradiation of amyloid β (Aβ), an amyloidogenic protein associated with Alzheimer’s disease. Using nuclear magnetic resonance spectroscopy, we observed gradual spectral changes in Aβ after a 10 s CAP pretreatment, which also suppressed its fibril formation, as revealed by thioflavin T assay. As per mass spectrometric analyses, these effects were attributed to selective oxidation of the methionine residue (Met) at position 35. Interestingly, this modification occurred when Aβ was dissolved into a pre-irradiated buffer, indicating that some reactive species oxidize the Met residue. Our results strongly suggest that the H2O2 generated in the solution by CAP irradiation is responsible for Met oxidation, which inhibits Aβ amyloid formation. The findings of the present study provide fundamental insights into plasma biology, giving clues for developing novel applications of CAP.
Mode of Action of 1-Naphthylphthalamic Acid in Conspicuous Local Stem Swelling of Succulent Plant, Bryophyllum calycinum: Relevance to the Aspects of Its Histological Observation and Comprehensive Analyses of Plant Hormones
Authors: Marasek-Ciolakowska Agnieszka , Dziurka Michał , Kowalska Urszula , Góraj-Koniarska Justyna , Saniewski Marian , Ueda Junichi , Miyamoto Kensuke
Published: March 2021

The mode of action of 1-naphthylphthalamic acid (NPA) to induce conspicuous local stem swelling in the area of its application to the growing internode in intact Bryophyllum calycinum was studied based on the aspects of histological observation and comprehensive analyses of plant hormones. Histological analyses revealed that NPA induced an increase in cell size and numerous cell divisions in the cortex and pith, respectively, compared to untreated stem. In the area of NPA application, vascular tissues had significantly wider cambial zones consisting of 5–6 cell layers, whereas phloem and xylem seemed not to be affected. This indicates that stem swelling in the area of NPA application is caused by stimulation of cell division and cell enlargement mainly in the cambial zone, cortex, and pith. Comprehensive analyses of plant hormones revealed that NPA substantially increased endogenous levels of indole-3-acetic acid (IAA) in the swelling area. NPA also increased endogenous levels of cytokinins, jasmonic acid, and its precursor, 12-oxo-phytodienoic acid, but did not increase abscisic acid and gibberellin levels. It was shown, using radiolabeled 14C-IAA, that NPA applied to the middle of internode segments had little effect on polar auxin transport, while 2,3,5-triiodobenzoic acid substantially inhibited it. These results strongly suggest that NPA induces changes in endogenous levels of plant hormones, such as IAA, cytokinins, and jasmonic acid, and their hormonal crosstalk results in a conspicuous local stem swelling. The possible different mode of action of NPA from other polar auxin transport inhibitors in succulent plants is extensively discussed.
Sperm Cholesterol Content Modifies Sperm Function and TRPV1-Mediated Sperm Migration
Authors: De Toni Luca , Sabovic Iva , De Filippis Vincenzo , Acquasaliente Laura , Peterle Daniele , Guidolin Diego , Sut Stefania , Di Nisio Andrea , Foresta Carlo , Garolla Andrea
Published: March 2021

Transient receptor potential channels-vanilloid receptor 1 (TRPV1) regulates thermotaxis in sperm-oriented motility. We investigated the role of membrane cholesterol (Chol) on TRPV1-mediated human sperm migration. Semen samples were obtained from five normozoospemic healthy volunteers. Sperm membrane Chol content, quantified by liquid chromatography-mass spectrometry, was modified by incubating cells with 2-hydroxypropyl-ß-cyclodextrin (CD) or the complex between CD and Chol (CD:Chol). The effect on sperm migration on a 10 μM capsaicin gradient (CPS), a TRPV1 agonist, was then investigated. Motility parameters were evaluated by Sperm Class Analyser. Intracellular calcium concentration and acrosome reaction were measured by staining with calcium orange and FITC-conjugated anti-CD46 antibody, respectively. TRPV1-Chol interaction was modelled by computational molecular-modelling (MM). CD and CD:Chol, respectively, reduced and increased membrane Chol content in a dose-dependent manner, resulting in a dose-dependent increase and reduction of sperm migration in a CPS gradient. MM confirmed a specific interaction of Chol with a TRPV1 domain that appeared precluded to the Chol epimer epicholesterol (Epi-Chol). Accordingly, CD:Epi-Chol was significantly less efficient than CD:Chol, in reducing sperm migration under CPS gradient. Chol inhibits TRPV1-mediated sperm function by directly interacting with a consensus sequence of the receptor.
Pluralism of News and Social Plurality in the Colombian Local Media
Authors: Molina-Rodríguez-Navas Pedro , Muñoz Lalinde Johamna
Published: March 2021

Information on the management of local administrations and the actions of the political leaders who govern them is essential for citizens to exercise their political rights. It is therefore necessary for these administrations to provide quality information that the media can use as sources for their news stories. At the same time, these media outlets have to compare and report while taking into account the plurality of their audiences. However, in local settings, collusion exists between political power and media owners that restricts the plurality of news, favoring the dominant political interests and hiding the demands, interests and protagonism of other social actors. We study this problem in the Caribbean Region of Colombia. We analyze the information that the town halls of the main cities in the region provide to the media and how the largest print newspapers and main regional television news broadcasters report on local politics. We compare these news stories to establish whether there is a plurality of news reports. In addition, we analyze the key elements of the news items disseminated by private media outlets to establish whether they report a limited vision of reality: the topics covered, the protagonists referred to in headlines and news stories, and the sources against which the news and images are compared. The results reveal shortcomings that result in similar information between public information and private media content, thus limiting the plurality of news reports and the social protagonism of other social agents. Ultimately, this hinders quality journalism that satisfies the interests of citizens.
Pre-Training on Mixed Data for Low-Resource Neural Machine Translation
Authors: Zhang Wenbo , Li Xiao , Yang Yating , Dong Rui
Published: March 2021

The pre-training fine-tuning mode has been shown to be effective for low resource neural machine translation. In this mode, pre-training models trained on monolingual data are used to initiate translation models to transfer knowledge from monolingual data into translation models. In recent years, pre-training models usually take sentences with randomly masked words as input, and are trained by predicting these masked words based on unmasked words. In this paper, we propose a new pre-training method that still predicts masked words, but randomly replaces some of the unmasked words in the input with their translation words in another language. The translation words are from bilingual data, so that the data for pre-training contains both monolingual data and bilingual data. We conduct experiments on Uyghur-Chinese corpus to evaluate our method. The experimental results show that our method can make the pre-training model have a better generalization ability and help the translation model to achieve better performance. Through a word translation task, we also demonstrate that our method enables the embedding of the translation model to acquire more alignment knowledge.
Spatio-Temporal Distribution and Fixed-Precision Sampling Plan of Scirtothrips dorsalis (Thysanoptera: Thripidae) in Florida Blueberry
Authors: Panthi Babu R. , Renkema Justin M. , Lahiri Sriyanka , Liburd Oscar E.
Published: March 2021

Scirtothrips dorsalis Hood is an invasive and foliar pest of Florida blueberry that reduces plant growth by feeding on new leaf growth. A sampling plan is needed to make informed control decisions for S. dorsalis in blueberry. Fourteen blueberry fields in central Florida were surveyed in 2017 and 2018 after summer pruning to determine the spatial and temporal distribution of S. dorsalis and to develop a fixed-precision sampling plan. A sampling unit of ten blueberry shoots (with four to five leaves each) was collected from one blueberry bush at each point along a 40 × 40 m grid. Field counts of S. dorsalis varied largely ranging from zero to 1122 adults and larvae per sampling unit. Scirtothrips dorsalis had aggregated distribution that was consistent within fields and temporally stable between summers, according to Taylor’s power law (TPL) (aggregation parameter, b = 1.57), probability distributions (56 out of 70 sampling occasions fit the negative binomial distribution), Lloyd’s index (b > 1 in 94% occasions), and Spatial Analysis by Distance IndicEs (31% had significant clusters). The newly developed fixed-precision sampling plan required 167, 42, seven, or three sampling units to estimate a nominal mean density of 20 S. dorsalis per sampling unit with a precision of 5%, 10%, 25%, or 40%, respectively. New knowledge on S. dorsalis distribution will aid in evaluating the timing and effectiveness of control measures.
Old Parasitoids for New Mealybugs: Host Location Behavior and Parasitization Efficacy of Anagyrus vladimiri on Pseudococcus comstocki
Authors: Ricciardi Renato , Zeni Valeria , Michelotti Davide , Di Giovanni Filippo , Cosci Francesca , Canale Angelo , Zang Lian-Sheng , Lucchi Andrea , Benelli Giovanni
Published: March 2021

The Comstock mealybug, Pseudococcus comstocki (Hemiptera: Pseudococcidae) is a primary pest of orchards in the North and Northwest of China. This pest appeared recently in Europe, including Italy, where it is infesting mainly vineyards as well as apple and pear orchards. The present study investigated the efficacy of Anagyrus vladimiri, a known biological control agent (BCA) of Planococcus ficus, on P. comstocki to evaluate a potential use for the management of this new pest. No-choice tests were conducted to quantify the parasitoid behavior against P. ficus and P. comstocki. The parasitoid successfully parasitized both species (parasitization rate: 51% and 67% on P. comstocki and P. ficus, respectively). The A.vladimiri developmental time (19.67 ± 1.12 vs. 19.70 ± 1.07 days), sex ratio (1.16 ± 1.12 vs. 1.58 ± 1.07) and hind tibia length of the progeny showed no differences when P. comstocki and P. ficus, respectively, were exploited as hosts. Two-choice tests, conducted by providing the parasitoid with a mixed population of P. ficus and P. comstocki, showed no host preference for either of the two mealybug species (23 vs. 27 first choices on P. comstocki and P. ficus, respectively). The parasitization rate (61.5% and 64.5% in P. comstocki and P. ficus, respectively) did not differ between the two hosts. Overall, our study adds basic knowledge on parasitoid behavior and host preferences and confirms the use of this economically important encyrtid species as an effective BCA against the invasive Comstock mealybug.
The Destructive Tree Pathogen Phytophthora ramorum Originates from the Laurosilva Forests of East Asia
Authors: Jung Thomas , Horta Jung Marília , Webber Joan F. , Kageyama Koji , Hieno Ayaka , Masuya Hayato , Uematsu Seiji , Pérez-Sierra Ana , Harris Anna R. , Forster Jack , Rees Helen , Scanu Bruno , Patra Sneha , Kudláček Tomáš , Janoušek Josef , Corcobado Tamara , Milenković Ivan , Nagy Zoltán , Csorba Ildikó , Bakonyi József , Brasier Clive M.
Published: March 2021

As global plant trade expands, tree disease epidemics caused by pathogen introductions are increasing. Since ca 2000, the introduced oomycete Phytophthora ramorum has caused devastating epidemics in Europe and North America, spreading as four ancient clonal lineages, each of a single mating type, suggesting different geographical origins. We surveyed laurosilva forests for P. ramorum around Fansipan mountain on the Vietnam-China border and on Shikoku and Kyushu islands, southwest Japan. The surveys yielded 71 P. ramorum isolates which we assigned to eight new lineages, IC1 to IC5 from Vietnam and NP1 to NP3 from Japan, based on differences in colony characteristics, gene x environment responses and multigene phylogeny. Molecular phylogenetic trees and networks revealed the eight Asian lineages were dispersed across the topology of the introduced European and North American lineages. The deepest node within P. ramorum, the divergence of lineages NP1 and NP2, was estimated at 0.5 to 1.6 Myr. The Asian lineages were each of a single mating type, and at some locations, lineages of “opposite” mating type were present, suggesting opportunities for inter-lineage recombination. Based on the high level of phenotypic and phylogenetic diversity in the sample populations, the coalescence results and the absence of overt host symptoms, we conclude that P. ramorum comprises many anciently divergent lineages native to the laurosilva forests between eastern Indochina and Japan.