bluuebunny/crossref_metadata_2025 huggingface dataset:
NISQ circuit compilation is the travelling salesman problem on a torus
Authors: Paler Alexandru , Zulehner Alwin , Wille Robert
Published: February 2021

Noisy, intermediate-scale quantum (NISQ) computers are expected to execute quantum circuits of up to a few hundred qubits. The circuits have to conform to NISQ architectural constraints regarding qubit allocation and the execution of multi-qubit gates. Quantum circuit compilation (QCC) takes a nonconforming circuit and outputs a compatible circuit. Can classical optimisation methods be used for QCC? Compilation is a known combinatorial problem shown to be solvable by two types of operations: (1) qubit allocation, and (2) gate scheduling. We show informally that the two operations form a discrete ring. The search landscape of QCC is a two dimensional discrete torus where vertices represent configurations of how circuit qubits are allocated to NISQ registers. Torus edges are weighted by the cost of scheduling circuit gates. The novelty of our approach uses the fact that a circuit’s gate list is circular: compilation can start from any gate as long as all the gates will be processed, and the compiled circuit has the correct gate order. Our work bridges a theoretical and practical gap between classical circuit design automation and the emerging field of quantum circuit optimisation.
A bin and hash method for analyzing reference data and descriptors in machine learning potentials
Authors: Paleico Martín Leandro , Behler Jörg
Published: February 2021

In recent years the development of machine learning potentials (MLPs) has become a very active field of research. Numerous approaches have been proposed, which allow one to perform extended simulations of large systems at a small fraction of the computational costs of electronic structure calculations. The key to the success of modern MLPs is the close-to first principles quality description of the atomic interactions. This accuracy is reached by using very flexible functional forms in combination with high-level reference data from electronic structure calculations. These data sets can include up to hundreds of thousands of structures covering millions of atomic environments to ensure that all relevant features of the potential energy surface are well represented. The handling of such large data sets is nowadays becoming one of the main challenges in the construction of MLPs. In this paper we present a method, the bin-and-hash (BAH) algorithm, to overcome this problem by enabling the efficient identification and comparison of large numbers of multidimensional vectors. Such vectors emerge in multiple contexts in the construction of MLPs. Examples are the comparison of local atomic environments to identify and avoid unnecessary redundant information in the reference data sets that is costly in terms of both the electronic structure calculations as well as the training process, the assessment of the quality of the descriptors used as structural fingerprints in many types of MLPs, and the detection of possibly unreliable data points. The BAH algorithm is illustrated for the example of high-dimensional neural network potentials using atom-centered symmetry functions for the geometrical description of the atomic environments, but the method is general and can be combined with any current type of MLP.
Introduction: Adaptation and the Battle of, and for, and over, and within the Public Humanities
Authors: Leitch Thomas
Published: February 2021

This introduction to the special issue of Adaptation devoted to adaptation and the public humanities focuses on the ways the once-anodyne term ‘public humanities’ has become more sharply politicized and contested over the past few years. In many ways, adaptation, which generates new versions and new readings of old texts instead of cancelling, erasing, or unpublishing them, offers the possibility of transcending the conflicts in contemporary culture. But the creation and the study of adaptations offer not a retreat from the culture wars but an array of new tools for waging them more productively by reframing them in ways that lead to more open and fruitful dialogue on the subjects proposed by the essays in this issue: theatrical performances cast for the public good, the costs of performing adapted versions of oneself or of encouraging adaptation-induced tourism, the ecological implications of adaptation, and the shifting valence of adaptation when it is practiced by public figures and posthuman agents.
Smoking Is Associated With Basal Cell Hyperplasia on Prostate Biopsy Specimens
Authors: Freitas Daniel Melecchi , Andriole Gerald L , Freedland Stephen J , Neto Brasil S , Moreira Daniel M
Published: February 2021

Objectives

To the best of our knowledge, no study has analyzed the association between cigarette smoking and prostate basal cell proliferation. Therefore, we sought to evaluate whether smoking status is associated with the presence of basal cell hyperplasia (BCH).

Methods

We performed a retrospective analysis of 8,196 men aged 50 to 75 years with prostate-specific antigen values between 2.5 µg/mL and 10 µg/mL and prior negative biopsy who were enrolled in the (REDUCE) trial. Cigarette smoking status was divided into current, former, or never categories at enrollment. The association between smoking and baseline BCH was evaluated, with logistic regression in univariable and multivariable analysis.

Results

A total of 1,233 (15.1%) men were current smokers, 3,206 (39.1%) were former smokers, and 3,575 (45.8%) were never smokers. In univariable analysis, current smoking was associated with higher baseline BCH occurrence compared with never (odds ratio [OR], 1.87; 95% confidence interval [CI], 1.14-3.10) and former smokers (OR, 1.77; 95% CI, 1.06-2.95). Similar results were found after adjusting for patient characteristics (current vs never smokers: OR, 1.92; 95% CI, 1.14-3.26; current vs former smokers: OR, 1.71; 95% CI, 1.01-2.91).

Conclusions

Among men undergoing prostate biopsy, all of whom had a negative biopsy result, current smoking at enrollment was independently associated with BCH in standard peripheral zone prostate biopsies.
Functional design of glycan-conjugated molecules using a chemoenzymatic approach
Authors: Ogata Makoto
Published: February 2021

Carbohydrates play important and diverse roles in the fundamental processes of life. We have established a method for accurately and a large-scale synthesis of functional carbohydrates with diverse properties using a unique enzymatic method. Furthermore, various artificial glycan-conjugated molecules have been developed by adding these synthetic carbohydrates to macromolecules and to middle- and low-molecular-weight molecules with different properties. These glycan-conjugated molecules have biological activities comparable to or higher than those of natural compounds and present unique functions. In this review, several synthetic glycan-conjugated molecules are taken as examples to show design, synthesis, and function.
General ways to improve false coverage rate-adjusted selective confidence intervals
Authors: Zhao Haibing
Published: February 2021

Post-selection inference on thousands of parameters has attracted considerable research interest in recent years. Specifically, Benjamini & Yekutieli (2005) considered constructing confidence intervals after selection. They proposed adjusting the confidence levels of marginal confidence intervals for the selected parameters to ensure control of the false coverage-statement rate. However, although Benjamini–Yekutieli confidence intervals are widely used, they are uniformly inflated. In this article, two methods for narrowing the Benjamini–Yekutieli confidence intervals are proposed. The first improves the confidence intervals by incorporating the selection event into the calculation. The second method further narrows those confidence intervals in which some parameters are selected with very small probabilities, which results in underutilization of the target level for control of the false coverage-statement rate. A breast cancer dataset is analysed to compare the methods.
Risk factors and impact of early anastomotic biliary complications after liver transplantation: UK registry analysis
Authors: Tingle S J , Thompson E R , Ali S S , Figueiredo R , Hudson M , Sen G , White S A , Manas D M , Wilson C H
Published: February 2021

Background

Biliary leaks and anastomotic strictures are common early anastomotic biliary complications (EABCs) following liver transplantation. However, there are no large multicentre studies investigating their clinical impact or risk factors. This study aimed to define the incidence, risk factors and impact of EABC.

Methods

The NHS registry on adult liver transplantation between 2006 and 2017 was reviewed retrospectively. Adjusted regression models were used to assess predictors of EABC, and their impact on outcomes.

Results

Analyses included 8304 liver transplant recipients. Patients with EABC (9·6 per cent) had prolonged hospitalization (23 versus 15 days; P < 0·001) and increased chance for readmission within the first year (56 versus 32 per cent; P < 0·001). Patients with EABC had decreased estimated 5-year graft survival of 75·1 versus 84·5 per cent in those without EABC, and decreased 5-year patient survival of 76·9 versus 83·3 per cent; both P < 0.001. Adjusted Cox regression revealed that EABCs have a significant and independent impact on graft survival (leak hazard ratio (HR) 1·344, P = 0·015; stricture HR 1·513, P = 0·002; leak plus stricture HR 1·526, P = 0·036) and patient survival (leak HR 1·215, P = 0·136, stricture HR 1·526, P = 0·001; leak plus stricture HR 1·509; P = 0·043). On adjusted logistic regression, risk factors for EABC included donation after circulatory death grafts, graft aberrant arterial anatomy, biliary anastomosis type, vascular anastomosis time and recipient model of end-stage liver disease.

Conclusion

EABCs prolong hospital stay, increase readmission rates and are independent risk factors for graft loss and increased mortality. This study has identified factors that increase the likelihood of EABC occurrence; research into interventions to prevent EABCs in these at-risk groups is vital to improve liver transplantation outcomes.
sangeranalyseR: Simple and Interactive Processing of Sanger Sequencing Data in R
Authors: Chao Kuan-Hao , Barton Kirston , Palmer Sarah , Lanfear Robert
Published: February 2021

sangeranalyseR is feature-rich, free, and open-source R package for processing Sanger sequencing data. It allows users to go from loading reads to saving aligned contigs in a few lines of R code by using sensible defaults for most actions. It also provides complete flexibility for determining how individual reads and contigs are processed, both at the command-line in R and via interactive Shiny applications. sangeranalyseR provides a wide range of options for all steps in Sanger processing pipelines including trimming reads, detecting secondary peaks, viewing chromatograms, detecting indels and stop codons, aligning contigs, estimating phylogenetic trees, and more. Input data can be in either ABIF or FASTA format. sangeranalyseR comes with extensive online documentation and outputs aligned and unaligned reads and contigs in FASTA format, along with detailed interactive HTML reports. sangeranalyseR supports the use of colorblind-friendly palettes for viewing alignments and chromatograms. It is released under an MIT licence and available for all platforms on Bioconductor (https://bioconductor.org/packages/sangeranalyseR, last accessed February 22, 2021) and on Github (https://github.com/roblanf/sangeranalyseR, last accessed February 22, 2021).
Legionella spp. All Ears? The Broad Occurrence of Quorum Sensing Elements outside Legionella pneumophila
Authors: Herran Benjamin , Grève Pierre , Berjeaud Jean-Marc , Bertaux Joanne , Crépin Alexandre
Published: February 2021

Legionella spp. are ubiquitous bacteria principally found in water networks and ∼20 species are implicated in Legionnaire’s disease. Among them, Legionella pneumophila is an intracellular pathogen of environmental protozoa, responsible for ∼90% of cases in the world. Legionella pneumophila regulates in part its virulence by a quorum sensing system named “Legionella quorum sensing,” composed of a signal synthase LqsA, two histidine kinase membrane receptors LqsS and LqsT and a cytoplasmic receptor LqsR. To date, this communication system was only found in L. pneumophila. Here, we investigated 58 Legionella genomes to determine the presence of a lqs cluster or homologous receptors using TBlastN. This analysis revealed three categories of species: 19 harbored a complete lqs cluster, 20 did not possess lqsA but maintained the receptor lqsR and/or lqsS, and 19 did not have any of the lqs genes. No correlation was observed between pathogenicity and the presence of a quorum sensing system. We determined by RT-qPCR that the lqsA gene was expressed at least in four strains among different species available in our laboratory. Furthermore, we showed that the lqs genomic region was conserved even in species possessing only the receptors of the quorum sensing system, indicating an ancestral acquisition and various loss dynamics during evolution. This system could therefore function in interspecific communication as well.
Ratings of Perceived Exertion During Walking: Predicting Major Mobility Disability and Effect of Structured Physical Activity in Mobility-Limited Older Adults
Authors: Cenko Erta , Chen Haiying , Gill Thomas M , Glynn Nancy W , Henderson Rebecca M , King Abby C , Pahor Marco , Qiu Peihua , Rego Alvito , Reid Kieran F , Tudor-Locke Catrine , Valiani Vincenzo , You Lu , Manini Todd M
Published: February 2021

Background

This study evaluated the association between ratings of perceived exertion (RPE) of walking and major mobility disability (MMD), as well as their transitions in response to a physical activity (PA) compared to a health education (HE) program.

Methods

Older adults (n = 1633) who were at risk for mobility impairment were randomized to structured PA or HE programs. During a 400 m walk, participants rated exertion as “light” or “hard.” An MMD event was defined as the inability to walk 400 m. MMD events and RPE values were assessed every 6 months for an average of 2.6 years.

Results

Participants rating their exertion as “hard” had a nearly threefold higher risk of MMD compared with those rating their exertion as “light” (HR: 2.61, 95% CI: 2.19–3.11). The association was held after adjusting for disease conditions, depression, cognitive function, and walking speed (HR: 2.24, 95% CI: 1.87–2.69). The PA group was 25% more likely to transition from “light” to “hard” RPE than the HE group (HR: 1.25, 95% CI: 1.05–1.49). Additionally, the PA group was 27% (HR: 0.73, 95% CI: 0.55 – 0.97) less likely to transition from a “hard” RPE to inability to walk 400 m and was more likely to recover their ability to walk 400 m by transitioning to a “hard” RPE (HR: 2.10, 95% CI: 1.39–3.17) than the HE group.

Conclusions

Older adults rating “hard” effort during a standardized walk test were at increased risk of subsequent MMD. A structured PA program enabled walking recovery, but was more likely to increase transition from “light” to “hard” effort, which may reflect the greater capacity to perform the test.
Association Between Caregiver Depression and Elder Mistreatment—Examining the Moderating Effect of Care Recipient Neuropsychiatric Symptoms and Caregiver-Perceived Burden
Authors: Fang Boye , Liu Huiying , Yan Elsie
Published: February 2021

Objectives

To examine the association between caregiver (CG) depression and increase in elder mistreatment and to investigate whether change in care recipient (CR) neuropsychiatric symptoms (NPS) and change in CG-perceived burden influence this association.

Methods

Using 2-year longitudinal data, we analyzed a consecutive sample of 800 Chinese primary family CGs and their CRs with mild cognitive impairment or mild-to-moderate dementia recruited from the geriatric and neurological departments of 3 Grade-A hospitals in the People’s Republic of China. Participatory dyads were assessed between September 2015 and February 2016 and followed for 2 years.

Results

CG depression at baseline was associated with a sharper increase in psychological abuse and neglect. For CRs with increased NPS, having a depressed CG predicted a higher level of psychological abuse than for those CRs without NPS. For CGs with decreased burden, the level of depression was associated with a slower increase in neglect than for CGs who remained low burden.

Discussion

This study showed the differential impact of CG depression on the increase in elder mistreatment depending on the change in CR NPS and CG-perceived burden. The present findings provide valuable insights into the design of a systematic and integrative intervention protocol for elder mistreatment that simultaneously focuses on treating CG depression and perceived burden and CR NPS.
Federal Incentives to Reform Long-Term Care Under the Affordable Care Act: State Adoption of the Balancing Incentive Program, 2011–2014
Authors: Beauregard Lisa K , Miller Edward Alan
Published: February 2021

Objectives

The Balancing Incentive Program (BIP) was an optional program for states within the Patient Protection and Affordable Care Act to promote Medicaid-funded home and community-based services (HCBS) for older adults and persons with disabilities. Twenty-one states opted to participate in BIP, including several states steadfastly opposed to the health insurance provisions of the Affordable Care Act. This study focused on identifying what factors were associated with states’ participation in this program.

Methods

Event history analysis was used to model state adoption of BIP from 2011 to 2014. A range of potential factors was considered representing states’ economic, political, and programmatic conditions.

Results

The results indicate that states with a higher percentage of Democrats in the state legislature, fewer state employees per capita, and more nursing facility beds were more likely to adopt BIP. In addition, states with fewer home health agencies per capita, that devoted smaller proportions of Medicaid long-term care spending to HCBS, and that had more Money Follows the Person transitions were also more likely to pursue BIP.

Discussion

The findings highlight the role of partisanship, administrative capacity, and program history in state BIP adoption decisions. The inclusion of BIP in the Affordable Care Act may have deterred some states from participating in the program due to partisan opposition to the legislation. To encourage the adoption of optional HCBS programs, federal policymakers should consider the role of financial incentives, especially for states with limited bureaucratic capacity and that have made less progress rebalancing Medicaid long-term services and supports.
Limb-clasping, cognitive deficit and increased vulnerability to kainic acid-induced seizures in neuronal glycosylphosphatidylinositol deficiency mouse models
Authors: Kandasamy Lenin C , Tsukamoto Mina , Banov Vitaliy , Tsetsegee Sambuu , Nagasawa Yutaro , Kato Mitsuhiro , Matsumoto Naomichi , Takeda Junji , Itohara Shigeyoshi , Ogawa Sonoko , Young Larry J , Zhang Qi
Published: February 2021

Posttranslational modification of a protein with glycosylphosphatidylinositol (GPI) is a conserved mechanism exists in all eukaryotes. Thus far, >150 human GPI-anchored proteins have been discovered and ~30 enzymes have been reported to be involved in the biosynthesis and maturation of mammalian GPI. Phosphatidylinositol glycan biosynthesis class A protein (PIGA) catalyzes the very first step of GPI anchor biosynthesis. Patients carrying a mutation of the PIGA gene usually suffer from inherited glycosylphosphatidylinositol deficiency (IGD) with intractable epilepsy and intellectual developmental disorder. We generated three mouse models with PIGA deficits specifically in telencephalon excitatory neurons (Ex-M-cko), inhibitory neurons (In-M-cko) or thalamic neurons (Th-H-cko), respectively. Both Ex-M-cko and In-M-cko mice showed impaired long-term fear memory and were more susceptible to kainic acid-induced seizures. In addition, In-M-cko demonstrated a severe limb-clasping phenotype. Hippocampal synapse changes were observed in Ex-M-cko mice. Our Piga conditional knockout mouse models provide powerful tools to understand the cell-type specific mechanisms underlying inherited GPI deficiency and to test different therapeutic modalities.
Maternal seafood intake during pregnancy, prenatal mercury exposure and child body mass index trajectories up to 8 years
Authors: Papadopoulou Eleni , Botton Jérémie , Caspersen Ida Henriette , Alexander Jan , Eggesbø Merete , Haugen Margaretha , Iszatt Nina , Jacobsson Bo , Knutsen Helle Katrine , Meltzer Helle Margrete , Sengpiel Verena , Stratakis Nikos , Vejrup Kristine , Brantsæter Anne Lise
Published: February 2021

Background

Maternal seafood intake during pregnancy and prenatal mercury exposure may influence children’s growth trajectories.

Methods

This study, based on the Norwegian Mother, Father and Child Cohort Study (MoBa), includes 51 952 mother-child pairs recruited in pregnancy during 2002–08 and a subsample (n = 2277) with maternal mercury concentrations in whole blood. Individual growth trajectories were computed by modelling based on child’s reported weight and length/height from 1 month to 8 years. We used linear mixed-effects regression analysis and also conducted discordant-sibling analysis.

Results

Maternal lean fish was the main contributor to total seafood intake in pregnancy and was positively but weakly associated with child body mass index (BMI) growth trajectory. Higher prenatal mercury exposure (top decile) was associated with a reduction in child’s weight growth trajectory, with the estimates ranging from -130 g [95% Confidence Intervals (CI) = -247, -12 g] at 18 months to -608 g (95% CI = -1.102, -113 g) at 8 years. Maternal fatty fish consumption was positively associated with child weight and BMI growth trajectory, but only in the higher mercury-exposed children (P-interaction = 0.045). Other seafood consumption during pregnancy was negatively associated with child weight growth compared with no intake, and this association was stronger for higher mercury-exposed children (P-interaction = 0.004). No association was observed between discordant maternal seafood intake and child growth in the sibling analysis.

Conclusions

Within a population with moderate seafood consumption and low mercury exposure, we found that maternal seafood consumption in pregnancy was associated with child growth trajectories, and the direction of the association varied by seafood type and level of prenatal mercury exposure. Prenatal mercury exposure was negatively associated with child growth. Our findings on maternal seafood intake are likely non-causal.
Preterm birth, low fetal growth and risk of suicide in adulthood: a national cohort and co-sibling study
Authors: Crump Casey , Sundquist Jan , Kendler Kenneth S , Edwards Alexis C , Sundquist Kristina
Published: February 2021

Background

Adverse perinatal exposures have been associated with psychiatric disorders and suicidal behaviours later in life. However, the independent associations of gestational age at birth or fetal growth with suicide death, potential sex-specific differences, and causality of these associations are unclear.

Methods

A national cohort study was conducted of all 2 440 518 singletons born in Sweden during 1973–98 who survived to age 18 years, who were followed up through 2016. Cox regression was used to compute hazard ratios (HRs) for suicide death associated with gestational age at birth or fetal growth while mutually adjusting for these factors, sociodemographic characteristics and family history of suicide. Co-sibling analyses assessed the influence of unmeasured shared familial (genetic and/or environmental) factors.

Results

In 31.2 million person-years of follow-up, 4470 (0.2%) deaths by suicide were identified. Early preterm birth (22–33 weeks) was associated with an increased risk of suicide among females [adjusted hazard ratio (HR), 1.97; 95% confidence interval CI), 1.29, 3.01; P = 0.002) but not males (0.90; 0.64, 1.28; P = 0.56), compared with full-term birth (39–41 weeks). Small for gestational age was associated with a modestly increased risk of suicide among females (adjusted HR, 1.27; 95% CI, 1.08, 1.51; P = 0.005) and males (1.14; 1.03, 1.27; P = 0.02). However, these associations were attenuated and non-significant after controlling for shared familial factors.

Conclusions

In this large national cohort, preterm birth in females and low fetal growth in males and females were associated with increased risks of suicide death in adulthood. However, these associations appeared to be non-causal and related to shared genetic or prenatal environmental factors within families.
Who Is a Refugee? Uncertainty and Discretion in Asylum Decisions
Authors: Liodden Tone Maia
Published: February 2021

Assessing claims for refugee status is a task often riddled with uncertainties, not least because of the challenge of establishing the credibility of the claims. The uncertainties enable divergent interpretations of both evidence and legal rules, thereby constituting a space for discretion in the refugee status determination process. This article explores how decision makers in the Norwegian asylum system reached a conviction about the outcome of asylum claims, despite numerous uncertainties. Decision makers who worked closely together developed a system of distinction that enabled them to single out applicants with protection needs from those who were not considered to be eligible. This system ordered the space for discretion, thereby reducing doubts about the outcome. It was based on the law and other formal sources, but also on recognizing patterns of difference and similarity with previous decisions. The emphasis on comparison between cases meant that the outcome of an individual application could not be understood in isolation; the distinctions between applicants who were accepted and those who were rejected depended, in part, on the case set as a whole. The findings suggest that, in a context of uncertainty, refugee status is to some extent determined by producing a local yardstick of who ‘the refugee’ is.
A validated asymptotic thermomechanical model for air-gap formation in tapered moulds in the continuous casting of steel
Authors: Devine K M , Vynnycky M , Mitchell S L , O’Brien S B G
Published: February 2021

A recent asymptotics-based thermomechanical model is adapted and applied to the mould region in the continuous casting of round steel billets, with a view to describing the complex interplay between air-gap formation, mould taper, cooling channel width and cooling water velocity. Although the situation is steady state, the analysis leads to what is mathematically a dual moving-boundary problem for the solid–melt and solid–air interfaces, where the distance from the top of the mould region is the time-like variable in the problem. Moreover, the two interfaces are initiated at different locations. In addition, the thermal and mechanical problems are found to decouple and it is possible to solve the first ahead of the second. The model equations are solved numerically using a finite-difference method, and the approach is subsequently successfully validated against a previous finite-element model and experimental data from temperature measurements taken within the mould.
ICMR’s Antimicrobial Resistance Surveillance system (i-AMRSS): a promising tool for global antimicrobial resistance surveillance
Authors: Kaur Jasmine , Dhama Ajay Singh , Buttolia Harish , Kaur Jasleen , Walia Kamini , Ohri Vinod , Kumar Vinit , Lynn Andrew M , Srivastava Alok , Singh Harpreet
Published: February 2021

Background

Growing resistance to antimicrobials has become an important health issue of the 21st century. Many international, national and local approaches are being employed for the control and prevention of antimicrobial resistance (AMR). Among them, surveillance is reported to be the best method to reduce the spread of infection and thereby AMR. An integral component of AMR surveillance is the informatics suite for collection, storage and analysis of surveillance data.

Methods

Considering the traits of an optimal surveillance tool and constraints with existing tools, Indian Council of Medical Research (ICMR) initiated the design and development of ICMR’s Antimicrobial Resistance Surveillance system (i-AMRSS). i-AMRSS is a web-based tool built using modular architecture. It is capable of collecting standardized data from small laboratories to generate local and nationwide reports.

Results

i-AMRSS is a robust, comprehensive, modular, extensible and intelligent open-source tool piloted in ICMR’s AMR Network (31 hospitals and laboratories across India) since 2016. The developed tool has collected more than 280 000 patient records to date.

Conclusions

The standardized data collected through i-AMRSS would be valuable for various collaborators to monitor outbreaks and infection control practices, evaluate transmission dynamics and formulate antibiotic use and selling policies. The tool is presently being used to capture human testing and consumption data, however, it can be extended for AMR surveillance using a ‘One Health’ approach.
Digestibility and safety of dry black soldier fly larvae meal and black soldier fly larvae oil in dogs
Authors: Freel Tarra A , McComb Alejandra , Koutsos Elizabeth A
Published: February 2021

Two trials were conducted to assess the acceptance, safety and digestibility of diets containing various inclusion levels of partially defatted black soldier fly (Hermetia illucens) larvae (BSFL) meal and BSFL oil by dogs. In trial 1, 5 extruded diets were evaluated for acceptance in adult Beagle dogs (n = 20; 10 male, 10 female) during a 48-hr period. Diets contained graded levels of BSFL meal (5.0%, 10.0%, and 20.0%), or graded levels of BSFL oil (2.5% and 5.0%), and all diets were well accepted. Thus, a digestibility trial (trial 2) was run with 56 adult dogs (16 male, 40 female) allocated into 7 dietary treatments; dogs were offered an extruded control diet containing no BSFL meal or oil, or extruded diets where BSFL meal partially replaced poultry by-product meal and corn meal at dietary levels of 5%, 10%, or 20% inclusion, or diets with BSFL oil partially replacing poultry fat at a 1:1 ratio at levels of 1%, 2.5%, or 5% inclusion. The treatment diets were fed for 28 d, during which time dogs were monitored for health (via physical examinations, clinical observations, and blood chemistry and hematology) and ingredient evaluation (via body weight, feed consumption, stool observation, and fecal nutrient apparent total tract digestibility). There were no significant differences in body weight or food consumption between treatment groups (P > 0.05) and daily observations indicated that the general health of the animals was maintained throughout the study. Stool quality was maintained at 3.2 to 3.4 (on a 5-point scale with a score of 1 being watery diarrhea and a score of 5 being hard, dry, and crumbly) per treatment group over the fecal observation period (days 22 to 27), indicating a well formed, sticky stool. All group mean hematology and blood chemistry parameters remained within normal limits for dogs. Apparent total tract digestibility of dry matter, protein, fat, and calories was not affected by treatment (P > 0.05). In general, amino acid digestibility was not impacted by treatment although some minor changes were observed. Apparent total tract digestibility was high for all nutrients examined. Overall, it was concluded that BSFL meal and BSFL oil are well tolerated by dogs and their consumption results in no impact to physiology that would be concerning. Based on these data, BSFL meal and oil did not affect general health and could be included safely in dog diets.
Differences in copper and selenium metabolism between Angus (Bos taurus) and Brahman (Bos indicus) cattle
Authors: Ranches Juliana , Alves Rhaiza , Vedovatto Marcelo , Palmer Elizabeth A , Moriel Philipe , Arthington John D
Published: February 2021

A 2-yr study was conducted at the Range Cattle Research and Education Center, University of Florida – Institute of Food and Agricultural Sciences (IFAS) (Ona, FL), to evaluate differences in the metabolism of Cu and Se of Angus (Bos taurus) and Brahman (Bos indicus) cattle. Thirty-two pregnant beef cows (n = 8 Brahman and 8 Angus/yr) were enrolled in the study in the first trimester of gestation. This study consisted of three phases: 1) restriction (day 0 to 90), 2) supplementation (day 91 to 150), and 3) calving. During all three phases, cows were individually fed and housed in partially covered drylot pens. During the restriction and supplementation phases, cows were provided a 1.5 kg/d of a grain-based concentrate supplement, which was fortified with flowers of S (50 g of supplemental S/cow daily; restriction phase) or Cu and Se (100 and 3 mg/d of Cu and Se, respectively; supplementation phase). Blood and liver samples were collected from all cows at 30 d intervals and from both cows and calves within 24 h of calving. Colostrum and milk samples were collected at calving and 7 d after birth. All data were analyzed using the MIXED procedure of SAS, where cow and calf were the experimental unit. During the restriction phase, a breed × day effect (P = 0.03) was observed where Brahman had greater liver Cu concentration than Angus cows in all sampling days. For liver Se concentration, a tendency (P = 0.07) for a breed effect was observed where Angus cows tended to have greater liver Se concentration than Brahman. During the supplementation phase, breed (P < 0.001) and day (P < 0.01) effects were observed, where Brahman cows had greater liver Cu concentration than Angus. For liver Se concentration, a day effect (P < 0.001) was observed, where liver Se concentration increased (P < 0.001) from day 90 to 120 and remained unchanged (P = 0.86) until day 150. At calving, no effects of breed (P = 0.34) were observed for liver Cu concentration of cows; however, Brahman calves tended (P = 0.09) to have greater liver Cu concentration than Angus calves. For Se liver concentration at calving, Angus cows tended (P = 0.07) to have greater liver Se concentration than Brahman cows; however, no breed differences (P = 0.70) were observed for liver Se concentration of calves at birth. In summary, substantial differences in multiple indicators of Cu and Se status were observed between Angus and Brahman cattle, implying that Angus and Brahman cattle possibly have different mechanisms to maintain adequate Cu and Se status.
Genetic and phenotypic parameters for body weights, harvest length, and growth curve parameters in American mink
Authors: Do Duy Ngoc , Hu Guoyu , Salek Ardestani Siavash , Miar Younes
Published: February 2021

Understanding the genetics underlying growth curve is important for selection of animals with better growth potential, but little is known about the genetics of growth curve parameters in mink. This study estimated the genetic parameters for body weights (BWs), harvest length (HL), and growth parameters derived from the Richards model. For this purpose, individual BW of 1,088 mink measured seven times in 3-wk intervals (weeks 13 to 31 of life) were used for growth curve modeling using the Richards model. The BW traits included BW at week 13 (BW13), 16 (BW16), 19 (BW19), 22 (BW22), 25 (BW25), 28 (BW28), and 31 (BW31). Univariate analyses indicated that sex and birth-year had significant effects (P < 0.05) on BW, HL, asymptotic weight (α), growth rate at mature (k), shape parameter (m), weight at the inflection point (WIP), and age at the inflection point (AIP). In contrast, the color type had only significant effect (P < 0.05) on BW31 and HL. Estimated heritabilities (±SE) were ranged from 0.36 ± 0.13 (BW13) to 0.46 ± 0.10 (BW22) for BW and were 0.51 ± 0.09, 0.29 ± 0.09, 0.30 ± 0.09, 0.33 ± 0.1, 0.44 ± 0.10, and 0.47 ± 0.10 for HL, α, k, m, WIP, and AIP, respectively. The parameter α had non-significant (P > 0.05) genetic correlations (±SE) with k (−0.21 ± 0.23) and m (−0.10 ± 0.22), suggesting that changing shape parameters (k and m) will not influence asymptotic weight (α). Strong significant (P < 0.05) phenotypic (from 0.46 ± 0.03 to 0.60 ± 0.03) and genetic (0.70±0.13 to 0.88±0.09) correlations were observed between HL and different BW measures. The α, AIP, and WIP parameters had significant (P < 0.05) genetic correlations with HL indicated that selection for higher α, AIP, and WIP values would increase HL. Parameters k and m had nonsignificant (P > 0.05) genetic correlations with HL, indicating the change of the curve shape could not influence HL. Overall, the results suggest that growth curve parameters are heritable and can respond to genetic or genomic selection for optimizing the performance in mink.
Necrotizing Esophagitis: A Big Squeeze?
Authors: Rakovich George , Rolland Sébastien
Published: February 2021

Necrotizing esophagitis is rare and poorly understood. The etiologies reported in what little has been published (i.e., gastroesophageal reflux exacerbated by gastric outlet obstruction and low-flow ischemia) seem somewhat simplistic and lack any direct evidence. The following paper illustrates a recent clinical case while laying out arguments supporting esophageal spasm as a possible contributing factor.
Effective approaches to discover new microbial metabolites in a large strain library
Authors: Zdouc Mitja M , Iorio Marianna , Vind Kristiina , Simone Matteo , Serina Stefania , Brunati Cristina , Monciardini Paolo , Tocchetti Arianna , Zarazúa Guadalupe S , Crüsemann Max , Maffioli Sonia I , Sosio Margherita , Donadio Stefano
Published: February 2021

Natural products have provided many molecules to treat and prevent illnesses in humans, animals and plants. While only a small fraction of the existing microbial diversity has been explored for bioactive metabolites, tens of thousands of molecules have been reported in the literature over the past 80 years. Thus, the main challenge in microbial metabolite screening is to avoid the re-discovery of known metabolites in a cost-effective manner. In this perspective, we report and discuss different approaches used in our laboratory over the past few years, ranging from bioactivity-based screening to looking for metabolic rarity in different datasets to deeply investigating a single Streptomyces strain. Our results show that it is possible to find novel chemistry through a limited screening effort, provided that appropriate selection criteria are in place.
Usefulness of endoscopic submucosal dissection for superficial esophageal squamous cell carcinoma in elderly patients: a single-center retrospective cohort study
Authors: Ishido Kenji , Tanabe Satoshi , Katada Chikatoshi , Kubota Yo , Furue Yasuaki , Wada Takuya , Watanabe Akinori , Koizumi Wasaburo
Published: February 2021

Background

The effectiveness of endoscopic treatment for superficial esophageal squamous cell carcinoma in the elderly is unclear.

Methods

We retrospectively studied efficacy and safety of endoscopic submucosal dissection for superficial esophageal squamous cell carcinoma in 358 patients at our hospital from July 2005 to December 2018. Patients were divided into elderly (≥75 years) and young (≤74 years) groups. Efficacy was evaluated based on overall survival and disease-specific survival, whereas safety was investigated based on the frequency of endoscopic submucosal dissection-related adverse events.

Results

The median observation period was 50 months. The elderly group comprised 111 patients, and young group comprised 247 patients. In the elderly and young groups, 76 (68.5%) and 159 (64.4%) underwent curative resection (P = 0.450), 8 (7.2%) and 34 (13.8%) underwent non-curative resection plus additional treatment and 12 (10.8%) and 15 (6.0%) underwent follow-up, respectively. The frequency of additional treatment for non-curative resection was significantly lower in the elderly group (P = 0.023). The 3-year overall survival of the elderly and young groups was 85.6 and 94.1%, respectively (P = 0.003). The 3-year disease-specific survival of the elderly and young groups was 98.4 and 98.5% (P = 0.682), respectively. The frequency of endoscopic submucosal dissection-related adverse events did not differ significantly between the groups (P = 0.581). The Charlson Comorbidity Index ≥2 was an independent prognostic factor for survival in the elderly group (P = 0.010; hazard ratio, 5.570; 95% confidence interval, 1.519–20.421).

Conclusions

Endoscopic submucosal dissection for superficial esophageal squamous cell carcinoma in elderly patients is as safe as that for young patients. The evaluation of Charlson Comorbidity Index was considered to help estimate the prognosis of elderly patients.
Mitotic rate in node-positive stage III melanoma: it might be as important a prognostic factor as node number
Authors: Tas Faruk , Erturk Kayhan
Published: February 2021

Background

Stage III melanoma is a heterogenous disease, and the number of tumor-involved lymph nodes is the most significantly unfavorable prognostic indicator for relapse and outcome. The aim of this study is to investigate the possible effects of the various clinicopathological factors on the course of node-positive stage III disease.

Methods

A total of 389 node-positive stage III cutaneous melanomas were included in the study and analyzed retrospectively. All underwent pathological nodal staging by sentinel lymph node biopsy or elective lymph node dissection.

Results

The group was male-dominant (59%) and the median age was 50 years. The largest group of patients was N1 (n = 221, 56.8%) followed by N2 (n = 105, 27.0%) and N3 (n = 63, 16.2%). N1 melanomas were less frequently associated with relapses than melanomas with multiple lymph node metastases (P = 0.05). The 5-year relapse-free survival rate was 37.9%. The melanomas with multiple lymph nodes metastases (P = 0.01), higher mitotic rate (P = 0.005) and ulceration (P = 0.02) had worse RFS. In the multivariate analysis only the significances of the N2–N3 stage (P = 0.016) and higher mitosis (P = 0.012) persisted. The severe lymph node metastasis (N2–N3) was associated with a higher mortality rate in comparison with the single nodal involvement (P = 0.05). The 5-year overall survival rate was 52.1%. Presence of relapse (P = 0.0001), higher mitotic rate (P = 0.03) and N2–N3 stage (P = 0.04) were inversely correlated with the overall survival. When relapse was included in the multivariate analysis, it was the only significant prognostic factor on survival (P = 0.0001), whereas mitosis became the only significant factor on survival with the exclusion of relapse from the multivariate analysis (P = 0.031).

Conclusion

In node-positive stage III melanoma, tumor mitotic rate might be just as significant a prognostic indicator as the metastatic lymph node number.
Perianal abscess due to a long fish bone: a case report
Authors: Ikeda Taro , Konaka Ryunosuke , Adachi Yukari , Matsumoto Akiko , Harada Naoki , Wada Takahiro , Mitsutsuji Masaaki , Samizo Masahiro
Published: February 2021

Few articles have reported cases of perianal abscess due to ingested foreign bodies. Herein, we report a case of perianal abscess due to a long fish bone.

A 72-year-old man who was toothless and wore a denture had a chief complaint of anal pain. His left-side buttock had swelling and redness. Computed tomography revealed a perianal abscess on his left-side buttock and high-intensity linear structure in the abscess cavity. We made a diagnosis of perianal abscess due to a fish bone and performed an emergency operation. We opened the abscess cavity and removed the 5 cm fish bone from the cavity. After drainage of the abscess cavity and antibiotic administration, he was discharged from our hospital on day 8.

A long fish bone could cause perianal abscesses. Rapid diagnosis and ensuring fish bone removal are important to prevent sepsis.
The Key Elements of Medical Negligence—Duty
Authors: Luther Gregg W
Published: February 2021

American tort law (a.k.a. negligence) is designed to be flexible and elastic to adapt to changes in time and public policy. It provides a structure of elements and factors to be applied to each case's specific facts on a case-by-case basis. The purpose of this structure is to achieve as much uniformity as possible in the application of tort law. One side effect is that this structure makes predicting the outcome difficult because of so many variables. In addition, there is no national tort law. Instead, each state has developed its own law in the area of torts, which has resulted in differing exceptions and requirements based on where the medical care was given. The purpose of this article is to explain the first element of a negligence case—“duty to use care”—and its accompanying factors/variables.

The first element that must be established in a medical negligence case is a duty to use care. In short, this means the physician must use a certain level of care in providing medical services. The physician typically owes her or his patient the duty to exercise care. However, there are special situations in which a physician may not owe a duty of care and thus cannot be held liable for medical negligence. This article is designed to provide an overview of the most common situations and summarizes the processes to determine whether a duty is owed.
A three-dimensional velocity of an erupting prominence prior to a coronal mass ejection
Authors: Gutierrez Maria V , Otsuji Kenichi , Asai Ayumi , Terrazas Raul , Ishitsuka Mutsumi , Ishitsuka Jose , Nakamura Naoki , Yoshinaga Yusuke , Morita Satoshi , Ishii Takako T , Ueno Satoru , Kitai Reizaburo , Shibata Kazunari
Published: February 2021

We present a detailed three-dimensional (3D) view of a prominence eruption, coronal loop expansion, and coronal mass ejections (CMEs) associated with an M4.4 flare that occurred on 2011 March 8 in the active region NOAA 11165. Full-disk Hα images of the flare and filament ejection were successfully obtained by the Flare Monitoring Telescope (FMT) following its relocation to Ica University, Peru. Multiwavelength observation around the Hα line enabled us to derive the 3D velocity field of the Hα prominence eruption. Features in extreme ultraviolet were also obtained by the Atmospheric Imager Assembly onboard the Solar Dynamic Observatory and the Extreme Ultraviolet Imager on board the Solar Terrestrial Relations Observatory - Ahead satellite. We found that, following collision of the erupted filament with the coronal magnetic field, some coronal loops began to expand, leading to the growth of a clear CME. We also discuss the succeeding activities of CME driven by multiple interactions between the expanding loops and the surrounding coronal magnetic field.
Christian Hospitality and the Case for Religious Refuge in Interregnum England
Authors: Fradkin Jeremy
Published: February 2021

This article shows how English supporters of Jewish immigration in the 1650s articulated a universal model of Christian hospitality for all foreigners fleeing religious persecution, regardless of whether they adhered to the Protestant faith of their English hosts. It thus urges a reconsideration of the widespread assumption that European Christians in this era were willing only to admit their own co-religionists as refugees. At the same time, however, the article points to the 1650s as a pivotal turning point in the relationship between the resettlement of refugees and the development of the British Empire. Severe crises of depopulation in newly conquered Ireland and Jamaica prompted a proliferation of schemes to resettle refugees in those two spaces. Empire, it was argued, would allow the English to be benevolent and charitable without having to share their own country with people different from themselves.