bluuebunny/crossref_metadata_2025 huggingface dataset:
Reduced Olfactory Bulb Volume in Obesity and Its Relation to Metabolic Health Status
Authors: Poessel Maria , Breuer Nora , Joshi Akshita , Pampel André , Villringer Arno , Hummel Thomas , Horstmann Annette
Published: November 2020

Smell perception plays an important role in eating behavior and might be involved in body weight gain. Since a body of literature implies that olfactory perception and function is hampered in obesity, we here investigate neuroanatomical correlates of this phenomenon. We assessed olfactory bulb (OB) volume with magnetic resonance imaging in 67 healthy participants with a body mass index (BMI) from 18.9 to 45.4 kg/m2 (mean = 28.58 ± 6.64). Moreover, we obtained psychophysiological data on olfactory ability (Sniffin’ Sticks, Food associated odor test) and self-report measurements on eating behavior. Additionally, we collected parameters associated with metabolic health in obesity (waist-hip ratio, waist-height ratio, leptin levels, body fat percentage, fat mass index, insulin resistance) to investigate recently proposed mechanistic explanatory models of why olfaction may be altered in obesity. We showed that OB volume was significantly lower in participants with obesity when compared to those of normal weight. Moreover, we found weak to moderate negative correlations between OB volume and BMI and related measures of metabolic health, especially leptin, body fat percentage, waist-height ratio and insulin resistance. However, neither OB volume nor BMI were related to olfactory function in our young and healthy sample. Nevertheless, our results provide first indications that obesity is associated with brain anatomical changes in the OBs.
Complications Related to Sexualized Drug Use: What Can We Learn From Literature?
Authors: Donnadieu-Rigole Hélène , Peyrière Hélène , Benyamina Amine , Karila Laurent
Published: November 2020

Chemsex is described as the use of specific psychoactive substances (PS) during sexual activity to sustain, enhance, disinhibit or facilitate the sexual experience. It preferentially concerns men who have sex with men (MSM). They use new synthetic substances like cathinones, methamphetamines, gamma-butyrolactone/gamma-hydroxybutyrate (GBL/GHB), ketamine, and cocaine. The prevalence of chemsex varies from 3 to 31% during lifetime. The Internet has participated significantly in the evolution of sexual behaviors, both in terms of sexual dating and the availability of new synthetic substances. The advent of geolocation applications contributed to the development of chemsex. The literature describes many complications linked to these sexual practices; the main clinical effects related to cathinones consumption were psychiatric symptoms; agitation, hallucinations, anxiety, suicidal ideation, paranoia, and confusion. Regular GBL/GHB consumption alter cognitive functions, particularly memory and emotion management. Use of these drugs in party and play is dramatically associated with high-risk sexual behaviors. The prevalence of hepatitis B, hepatitis C syphilis, and HIV is higher in men who use methamphetamine and Viagra and/or who declared they practiced slamming, chemsex, and fisting. Other sexually transmitted infections (STIs) such as gonorrhea have increased with methamphetamine and GHB/GBL use. Actually, the care of individuals who practice Chemsex in a problematic way is currently not codified, but the use of integrative and specific interventions is necessary.
Evaluation of a P300-Based Brain-Machine Interface for a Robotic Hand-Orthosis Control
Authors: Delijorge Jonathan , Mendoza-Montoya Omar , Gordillo Jose L. , Caraza Ricardo , Martinez Hector R. , Antelis Javier M.
Published: November 2020

This work presents the design, implementation, and evaluation of a P300-based brain-machine interface (BMI) developed to control a robotic hand-orthosis. The purpose of this system is to assist patients with amyotrophic lateral sclerosis (ALS) who cannot open and close their hands by themselves. The user of this interface can select one of six targets, which represent the flexion-extension of one finger independently or the movement of the five fingers simultaneously. We tested offline and online our BMI on eighteen healthy subjects (HS) and eight ALS patients. In the offline test, we used the calibration data of each participant recorded in the experimental sessions to estimate the accuracy of the BMI to classify correctly single epochs as target or non-target trials. On average, the system accuracy was 78.7% for target epochs and 85.7% for non-target trials. Additionally, we observed significant P300 responses in the calibration recordings of all the participants, including the ALS patients. For the BMI online test, each subject performed from 6 to 36 attempts of target selections using the interface. In this case, around 46% of the participants obtained 100% of accuracy, and the average online accuracy was 89.83%. The maximum information transfer rate (ITR) observed in the experiments was 52.83 bit/min, whereas that the average ITR was 18.13 bit/min. The contributions of this work are the following. First, we report the development and evaluation of a mind-controlled robotic hand-orthosis for patients with ALS. To our knowledge, this BMI is one of the first P300-based assistive robotic devices with multiple targets evaluated on people with ALS. Second, we provide a database with calibration data and online EEG recordings obtained in the evaluation of our BMI. This data is useful to develop and compare other BMI systems and test the processing pipelines of similar applications.
Identification and Characterization of Biomarkers and Their Role in Opioid Addiction by Integrated Bioinformatics Analysis
Authors: Zhang Xiuning , Yu Hailei , Bai Rui , Ma Chunling
Published: November 2020

Although numerous studies have confirmed that the mechanisms of opiate addiction include genetic and epigenetic aspects, the results of such studies are inconsistent. Here, we downloaded gene expression profiling information, GSE87823, from the Gene Expression Omnibus database. Samples from males between ages 19 and 35 were selected for analysis of differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) enrichment analyses were used to analyze the pathways associated with the DEGs. We further constructed protein-protein interaction (PPI) networks using the STRING database and used 10 different calculation methods to validate the hub genes. Finally, we utilized the Basic Local Alignment Search Tool (BLAST) to identify the DEG with the highest sequence similarity in mouse and detected the change in expression of the hub genes in this animal model using RT-qPCR. We identified three key genes, ADCY9, PECAM1, and IL4. ADCY9 expression decreased in the nucleus accumbens of opioid-addicted mice compared with control mice, which was consistent with the change seen in humans. The importance and originality of this study are provided by two aspects. Firstly, we used a variety of calculation methods to obtain hub genes; secondly, we exploited homology analysis to solve the difficult challenge that addiction-related experiments cannot be carried out in patients or healthy individuals. In short, this study not only explores potential biomarkers and therapeutic targets of opioid addiction but also provides new ideas for subsequent research on opioid addiction.
Blood Cell DNA Methylation of Aging-Related Ubiquitination Gene DZIP3 Can Predict the Onset of Early Stage Colorectal Cancer
Authors: Quan Yuan , Liang Fengji , Wu Deqing , Yao Xueqing , Hu Zhihuang , Zhu Yuexing , Chen Ying , Wu Andong , Tang Danian , Huang Bingyang , Xu Ruifeng , Lyu Zejian , Yan Qian , Luo Lang , Ning Zhengzhi , Li Yong , Xiong Jianghui
Published: November 2020

There is a body of evidence that the aging immune system is linked to cancer. In this study, with aging- and immune-related DNA methylation data, we investigated the DNA methylation regulation changes in promoters with other regions of genes during aging and their association with the immune-cell proportion in the circulating whole blood of individuals. The analyses for aging- and CD4+ T cell proportion-derived differential genes showed that ubiquitination plays an important role in the aging immune system and tumorigenesis. Therefore, starting from a set of pre-annotated ubiquitination genes, we found that among the differentially ubiquitinated genes, DZIP3, an E3 ubiquitin ligase with no reports on its function in immune cells and tumorigenesis, was significantly associated with both aging (P-value = 3.86e-06) and CD4+ T cell proportion (P-value = 1.97e-05) in circulating blood. By collecting a cohort of 100 colon cancer patients and 50 healthy individuals, we validated that the 1st exon DNA methylation of DZIP3 could predict the onset of early stage (AUC = 0.833, OR = 8.82) and all pTNM stages of colorectal cancer (AUC = 0.782, OR = 5.70). Thus, the epigenetically regulated ubiquitination machine plays an important role in immune aging and tumorigenesis.
Dysregulation of Long Non-coding RNAs and mRNAs in Plasma of Clear Cell Renal Cell Carcinoma Patients Using Microarray and Bioinformatic Analysis
Authors: Zhang Bing , Chu Wei , Wen Feifei , Zhang Li , Sun Lixia , Hu Baoguang , Wang Jingjing , Su Qingguo , Mei Yanhui , Cao Jingyuan , Zheng Jing , Mou Xiaodong , Dong Hongliang , Lin Xiaoyan , Wang Nan , Ji Hong
Published: November 2020

Objective: The roles of long non-coding RNAs (lncRNAs) in the diagnosis of clear cell renal cell carcinoma (ccRCC) are still not well-defined. We aimed to identify differentially expressed lncRNAs and mRNAs in plasma of ccRCC patients and health controls systematically.

Methods: Expression profile of plasma lncRNAs and mRNAs in ccRCC patients and healthy controls was analyzed based on microarray assay. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway-based approaches were used to investigate biological function and signaling pathways mediated by the differentially expressed mRNAs. SOCS2-AS1 was selected for validation using Real-Time PCR. The differentially expressed lncRNAs and mRNAs were further compared with E-MTAB-1830 datasets using Venn and the NetworkAnalyst website. The GEPIA and ULCAN websites were utilized for the evaluation of the expression level of differentially expressed mRNA and their association with overall survival (OS).

Results: A total of 3,664 differentially expressed lncRNAs were identified in the plasma of ccRCC patients, including 1,511 up-regulated and 2,153 down-regulated lncRNAs (fold change ≥2 and P < 0.05), respectively. There were 2,268 differentially expressed mRNAs, including 932 up-regulated mRNAs and 1,336 down-regulated mRNAs, respectively (fold change ≥2 and P < 0.05). Pathway analysis based on deregulated mRNAs was mainly involved in melanogenesis and Hippo signaling pathway (P < 0.05). In line with the lncRNA microarray findings, the SOCS2-AS1 was down-regulated in ccRCC plasma and tissues, as well as in cell lines. Compared with the E-MTAB-1830 gene expression profiles, we identified 18 lncRNAs and 87 mRNAs differently expressed in both plasma and neoplastic tissues of ccRCC. The expression of 10 mRNAs (EPB41L4B, CCND1, GGT1, CGNL1, CYSLTR1, PLAUR, UGT3A1, PROM2, MUC12, and PCK1) was correlated with the overall survival (OS) rate in ccRCC patients based on the GEPIA and ULCAN websites.

Conclusions: We firstly reported differentially expressed lncRNAs in ccRCC patients and healthy controls systemically. Several differentially expressed lncRNAs and mRNAs were identified, which might serve as diagnostic or prognostic markers. The biological function of these lncRNAs and mRNAs should be further validated. Our study may contribute to the future treatment of ccRCC and provide novel insights into cancer biology.
Dissecting the Lymphatic System to Predict Melanoma Metastasis
Authors: Suresh Rishi , Ziemys Arturas , Holder Ashley M.
Published: November 2020

Melanoma is the most lethal form of skin cancer in the United States. Current American Joint Committee on Cancer (AJCC) staging uses Breslow depth and ulceration as the two primary tumor factors that predict metastatic risk in cutaneous melanoma. Early disease stages are generally associated with high survival rates. However, in some cases, patients with thin melanomas develop advanced disease, suggesting other factors may contribute to the metastatic potential of an individual patient’s melanoma. This review focuses on the role of the lymphatic system in the metastasis of cutaneous melanoma, from recent discoveries in mechanisms of lymphangiogenesis to elements of the lymphatic system that ultimately may aid clinicians in determining which patients are at highest risk. Ultimately, this review highlights the need to integrate pathological, morphological, and molecular characteristics of lymphatics into a “biomarker” for metastatic potential.
microRNAs Biogenesis, Functions and Role in Tumor Angiogenesis
Authors: Annese Tiziana , Tamma Roberto , De Giorgis Michelina , Ribatti Domenico
Published: November 2020

microRNAs (miRNAs) are small non-coding RNA molecules, evolutionary conserved. They target more than one mRNAs, thus influencing multiple molecular pathways, but also mRNAs may bind to a variety of miRNAs, either simultaneously or in a context-dependent manner. miRNAs biogenesis, including miRNA transcription, processing by Drosha and Dicer, transportation, RISC biding, and miRNA decay, are finely controlled in space and time.

miRNAs are critical regulators in various biological processes, such as differentiation, proliferation, apoptosis, and development in both health and disease. Their dysregulation is involved in tumor initiation and progression. In tumors, they can act as onco-miRNAs or oncosuppressor-miRNA participating in distinct cellular pathways, and the same miRNA can perform both activities depending on the context.

In tumor progression, the angiogenic switch is fundamental. miRNAs derived from tumor cells, endothelial cells, and cells of the surrounding microenvironment regulate tumor angiogenesis, acting as pro-angiomiR or anti-angiomiR.

In this review, we described miRNA biogenesis and function, and we update the non-classical aspects of them. The most recent role in the nucleus, as transcriptional gene regulators and the different mechanisms by which they could be dysregulated, in tumor initiation and progression, are treated. In particular, we describe the role of miRNAs in sprouting angiogenesis, vessel co-option, and vasculogenic mimicry. The role of miRNAs in lymphoma angiogenesis is also discussed despite the scarcity of data.

The information presented in this review reveals the need to do much more to discover the complete miRNA network regulating angiogenesis, not only using high-throughput computational analysis approaches but also morphological ones.
Overexpression of miRNA-3613-3p Enhances the Sensitivity of Triple Negative Breast Cancer to CDK4/6 Inhibitor Palbociclib
Authors: Yu Yuanhang , Liao Han , Xie Rong , Zhang Yue , Zheng Renjing , Chen Jianying , Zhang Bo
Published: November 2020

Triple negative breast cancer (TNBC) is characterized by lack of expression of the estrogen and progesterone receptors and HER2, which are common therapeutic targets. CDK4/6 inhibitor Palbociclib has been approved as an anti-cancer agent for breast cancer. However, identifying biomarkers that predict the response to Palbociclib has always been a challenge for molecular targeted therapy. In this study, we identify microRNA as a hallmark in TNBC patients and explore if miR-3613-3p might serve as a tumor suppressor biomarker for triple negative breast cancer patients and if overexpression of miR-3613-3p could enhance the sensitivity of TNBC cells to Palbociclib. We show that the expression of miR3613-3p was down-regulated in TNBC tumors and cells, and the overexpression of miR-3613-3p in patients’ tumor tissues was clinically and pathologically correlated with favorable prognosis, such as smaller tumor size and the lower Ki-67.In vitro, overexpression of miR-3613-3p inhibited cell proliferation, induced G1 cell-cycle arrest, and enhanced the sensitivity of TNBC cells to Palbociclib treatment.In vivostudy revealed that overexpression of miR-3613-3p inhibited TNBC tumorigenesis and exerted a significant inhibitory effect of Palbociclib on MDA-MB-231 cells. Mechanically, SMAD2 and EZH2 were found to be two direct targets of miR-3613-3p and mediate the proliferation of TNBC cells and the sensitivity of the cells to Palbociclib through inducing cellular senescence. Our findings suggested that miR-3613-3p acts as a cancer-suppressor miRNA in TNBC. Moreover, our study showed that miR-3613-3p might be used as a predictive biomarker for the response of TNBC to Palbociclib.
Case Report: CMV-Associated Congenital Nephrotic Syndrome
Authors: Jacob Anju , Habeeb Shameer M. , Herlitz Leal , Simkova Eva , Shekhy Jwan F. , Taylor Alan , Abuhammour Walid , Abou Tayoun Ahmad , Bitzan Martin
Published: November 2020

Background:Congenital nephrotic syndrome, historically defined by the onset of large proteinuria during the first 3 months of life, is a rare clinical disorder, generally with poor outcome. It is caused by pathogenic variants in genes associated with this syndrome or by fetal infections disrupting podocyte and/or glomerular basement membrane integrity. Here we describe an infant with congenital CMV infection and nephrotic syndrome that failed to respond to targeted antiviral therapy. Case and literature survey highlight the importance of the “tetrad” of clinical, virologic, histologic, and genetic workup to better understand the pathogenesis of CMV-associated congenital and infantile nephrotic syndromes.

Case Presentation:A male infant was referred at 9 weeks of life with progressive abdominal distention, scrotal edema, and vomiting. Pregnancy was complicated by oligohydramnios and pre-maturity (34 weeks). He was found to have nephrotic syndrome and anemia, normal platelet and white blood cell count, no splenomegaly, and no syndromic features. Diagnostic workup revealed active CMV infection (positive CMV IgM/PCR in plasma) and decreased C3 and C4. Maternal anti-CMV IgG was positive, IgM negative. Kidney biopsy demonstrated focal mesangial proliferative and sclerosing glomerulonephritis with few fibrocellular crescents, interstitial T- and B-lymphocyte infiltrates, and fibrosis/tubular atrophy. Immunofluorescence was negative. Electron microscopy showed diffuse podocyte effacement, but no cytomegalic inclusions or endothelial tubuloreticular arrays. After 4 weeks of treatment with valganciclovir, plasma and urine CMV PCR were negative, without improvement of the proteinuria. Unfortunately, the patient succumbed to fulminant pneumococcal infection at 7 months of age. Whole exome sequencing and targeted gene analysis identified a novel homozygous, pathogenic variant (2071+1G>T) inNPHS1.

Literature Review and Discussion:The role of CMV infection in isolated congenital nephrotic syndrome and the corresponding pathological changes are still debated. A search of the literature identified only three previous reports of infants with congenital nephrotic syndrome and evidence of CMV infection, who also underwent kidney biopsy and genetic studies.

Conclusion:Complete workup of congenital infections associated with nephrotic syndrome is warranted for a better understanding of their pathogenesis (“diagnostic triad” of viral, biopsy, and genetic studies). Molecular testing is essential for acute and long-term prognosis and treatment plan.
Da-Chai-Hu Decoction Ameliorates High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Remodeling the Gut Microbiota and Modulating the Serum Metabolism
Authors: Cui Huantian , Li Yuting , Wang Yuming , Jin Lulu , Yang Lu , Wang Li , Liao Jiabao , Wang Haoshuo , Peng Yanfei , Zhang Zhaiyi , Wang Hongwu , Liu Xiangguo
Published: November 2020

The dysbiosis in gut microbiota could affect host metabolism and contribute to the development of nonalcoholic fatty liver disease (NAFLD). Da-Chai-Hu decoction (DCH) has demonstrated protective effects on NAFLD, however, the exact mechanisms remain unclear. In this study, we established a NAFLD rat model using a high fat diet (HFD) and provided treatment with DCH. The changes in gut microbiota post DCH treatment were then investigated using 16S rRNA sequencing. Additionally, serum untargeted metabolomics were performed to examine the metabolic regulations of DCH on NAFLD. Our results showed that DCH treatment improved the dyslipidemia, insulin resistance (IR) and ameliorated pathological changes in NAFLD model rats. 16S rRNA sequencing and untargeted metabolomics showed significant dysfunction in gut microbiota community and serum metabolites in NAFLD model rats. DCH treatment restored the dysbiosis of gut microbiota and improved the dysfunction in serum metabolism. Correlation analysis indicated that the modulatory effects of DCH on the arachidonic acid (AA), glycine/serine/threonine, and glycerophospholipid metabolic pathways were related to alterations in the abundance of Romboutsia, Bacteroides, Lactobacillus, Akkermansia, Lachnoclostridium and Enterobacteriaceae in the gut microflora. In conclusion, our study revealed the ameliorative effects of DCH on NAFLD and indicated that DCH’s function on NAFLD may link to the improvement of the dysbiosis of gut microbiota and the modulation of the AA, glycerophospholipid, and glycine/serine/threonine metabolic pathways.
Targeting Orphan Nuclear Receptors NR4As for Energy Homeostasis and Diabetes
Authors: Zhang Chenyang , Zhang Bin , Zhang Xuelian , Sun Guibo , Sun Xiaobo
Published: November 2020

Orphan nuclear receptors are important members of the nuclear receptor family and may regulate cell proliferation, metabolism, differentiation, and apoptosis. NR4As, a subfamily of orphan nuclear receptors, have been reported to play key roles in carbohydrate and lipid metabolism and energy homeostasis. Popularity of obesity has resulted in a series of metabolic diseases such as diabetes and its complications. While imbalance of energy intake and expenditure is the main cause of obesity, the concrete mechanism of obesity has not been fully understood. It has been reported that NR4As have significant regulatory effects on energy homeostasis and diabetes and are expected to become new targets for discovering drugs for metabolic syndrome. A number of studies have demonstrated that abnormalities in metabolism induced by altered levels of NR4As may contribute to numerous diseases, such as chronic inflammation, tumorigenesis, diabetes and its complications, atherosclerosis, and other cardiovascular diseases. However, systematic reviews focusing on the roles of NR4As in mediating energy homeostasis and diabetes remain limited. Therefore, this article reviews the structure and regulation of NR4As and their critical function in energy homeostasis and diabetes, as well as small molecules that may regulate NR4As. Our work is aimed at providing valuable support for the research and development of drugs targeting NR4As for the treatment of obesity and related metabolic diseases.
Tackle Epithelial-Mesenchymal Transition With Epigenetic Drugs in Cancer
Authors: Dong Bo , Qiu Zhaoping , Wu Yadi
Published: November 2020

Epithelial-mesenchymal Transition (EMT) is a de-differentiation process in which epithelial cells lose their epithelial properties to acquire mesenchymal features. EMT is essential for embryogenesis and wound healing but is aberrantly activated in pathological conditions like fibrosis and cancer. Tumor-associated EMT contributes to cancer cell initiation, invasion, metastasis, drug resistance and recurrence. This dynamic and reversible event is governed by EMT-transcription factors (EMT-TFs) with epigenetic complexes. In this review, we discuss recent advances regarding the mechanisms that modulate EMT in the context of epigenetic regulation, with emphasis on epigenetic drugs, such as DNA demethylating reagents, inhibitors of histone modifiers and non-coding RNA medication. Therapeutic contributions that improve epigenetic regulation of EMT will translate the clinical manifestation as treating cancer progression more efficiently.
Analysis of Stock Price Motion Asymmetry via Visibility-Graph Algorithm
Authors: Liu Ruiyun , Chen Yu
Published: November 2020

This paper is the first to differentiate between concave and convex price motion trajectories by applying visibility-graph and invisibility-graph algorithms to the analyses of stock indices. Concave and convex indicators for price increase and decrease motions are introduced to characterize accelerated and decelerated stock index increases and decreases. Upon comparing the distributions of these indicators, it is found that asymmetry exists in price motion trajectories and that the degree of asymmetry, which is characterized by the Kullback-Leibler divergence between the distributions of rise and fall indictors, fluctuates after a change in time scope. Moreover, asymmetry in price motion speeds is demonstrated by comparing conditional expected rise and fall returns on the node degrees of visibility and invisibility graphs.
Model of Continuous Random Cascade Processes in Financial Markets
Authors: Maskawa Jun-ichi , Kuroda Koji
Published: November 2020

This article presents a continuous cascade model of volatility formulated as a stochastic differential equation. Two independent Brownian motions are introduced as random sources triggering the volatility cascade: one multiplicatively combines with volatility; the other does so additively. Assuming that the latter acts perturbatively on the system, the model parameters are estimated by the application to an actual stock price time series. Numerical calculation of the Fokker–Planck equation derived from the stochastic differential equation is conducted using the estimated values of parameters. The results reproduce the probability density function of the empirical volatility, the multifractality of the time series, and other empirical facts.
Influence of the Concentration of Fe and Cu Nanoparticles on the Dynamics of the Size Distribution of Nanoparticles
Authors: Gudkov Sergey V. , Baimler Ilya V. , Uvarov Oleg V. , Smirnova Veronika V. , Volkov Mikhail Yu , Semenova Anastasia A. , Lisitsyn Andrey B.
Published: November 2020

The evolution of the size distribution of nanoparticles depending on the concentration of nanoparticles in a colloidal solution is investigated. The formation of new stable distributions shifted relative to the initial distribution is directly related to the processes of agglomeration of nanoparticles. Using successive two-fold dilutions of nanoparticles by 2–32 times, it was shown that the maximum of the nanoparticle size distribution shifts toward smaller sizes with a decrease in the concentration of nanoparticles, both for distributions by the number of nanoparticles and for distributions by mass of nanoparticles. Thus, with dilutions, the relative concentration of individual nanoparticles increases, while the number of particles in one aggregate decreases. A mathematical model has been created that predicts a change in distribution with a change in the concentration of nanoparticles in a colloid.
Acute Effects of Continuous and Intermittent Blood Flow Restriction on Movement Velocity During Bench Press Exercise Against Different Loads
Authors: Wilk Michal , Gepfert Mariola , Krzysztofik Michal , Stastny Petr , Zajac Adam , Bogdanis Gregory C.
Published: November 2020

This study evaluated the effects of continuous and intermittent blood flow restriction (BFR) with 70% of full arterial occlusion pressure on bar velocity during the bench press exercise against a wide range of resistive loads. Eleven strength-trained males (age: 23.5 ± 1.4 years; resistance training experience: 2.8 ± 0.8 years, maximal bench press strength – 1RM = 101.8 ± 13.9 kg; body mass = 79.8 ± 10.4 kg), performed three different testing protocols in random and counterbalanced order: without BFR (NO-BFR); intermittent BFR (I-BFR) and continuous BFR (C-BFR). During each experimental session, subjects performed eight sets of two repetitions each, with increasing loads from 20 to 90% 1RM (10% steps), and 3 min rest between each set. In the C-BFR condition occlusion was kept throughout the trial, while in the I-BFR, occlusion was released during each 3 min rest interval. Peak bar velocity (PV) during the bench press exercise was higher by 12–17% in both I-BFR and C-BFR compared with NO-BFR only at the loads of 20, 30, 40, and 50% 1RM (p < 0.001), while performance at higher loads remained unchanged. Mean bar velocity (MV) was unaffected by occlusion (p = 0.342). These results indicate that BFR during bench press exercise increases PV and this may be used as an enhanced stimulus during explosive resistance training. At higher workloads, bench press performance was not negatively affected by BFR, indicating that the benefits of exercise under occlusion can be obtained while explosive performance is not impaired.
Cardiovascular Effects of Caffeic Acid and Its Derivatives: A Comprehensive Review
Authors: Silva Henrique , Lopes Nuno Miguel F.
Published: November 2020

Caffeic acid (CA) and its phenethyl ester (CAPE) are naturally occurring hydroxycinnamic acids with an interesting array of biological activities; e.g., antioxidant, anti-inflammatory, antimicrobial and cytostatic. More recently, several synthetic analogs have also shown similar properties, and some with the advantage of added stability. The actions of these compounds on the cardiovascular system have not been thoroughly explored despite presenting an interesting potential. Indeed the mechanisms underlying the vascular effects of these compounds particularly need clarifying. The aim of this paper is to provide a comprehensive and up-to-date review on current knowledge about CA and its derivatives in the cardiovascular system. Caffeic acid, CAPE and the synthetic caffeic acid phenethyl amide (CAPA) exhibit vasorelaxant activity by acting on the endothelial and vascular smooth muscle cells. Vasorelaxant mechanisms include the increased endothelial NO secretion, modulation of calcium and potassium channels, and modulation of adrenergic receptors. Together with a negative chronotropic effect, vasorelaxant activity contributes to lower blood pressure, as several preclinical studies show. Their antioxidant, anti-inflammatory and anti-angiogenic properties contribute to an important anti-atherosclerotic effect, and protect tissues against ischemia/reperfusion injuries and the cellular dysfunction caused by different physico-chemical agents. There is an obvious shortage ofin vivostudies to further explore these compounds’ potential in vascular physiology. Nevertheless, their favorable pharmacokinetic profile and overall lack of toxicity make these compounds suitable for clinical studies.
Therapeutic Relationship and Dropout in High-Risk Adolescents’ Intensive Group Psychotherapeutic Programme
Authors: Hauber Kirsten , Boon Albert , Vermeiren Robert
Published: November 2020

Objective

Dropout rates are a prominent problem in youth psychotherapy. An important determinant of dropouts is the quality of the therapeutic relationship. This study aimed to evaluate the association between the therapeutic relationship and dropouts in an intensive mentalization-based treatment (MBT) for adolescents with personality disorders.

Methods

Patients (N = 105) included were either dropouts (N = 36) or completers (N = 69) of an intensive MBT. The therapeutic relationship was measured with the child version of the Session Rating Scale (C-SRS), which was completed by the patient after each group therapy session. For each patient, the treatment termination status (dropout or completer) was indicated by the treatment staff. The reliable change index (RCI) was calculated for the C-SRS to determine significant changes in the therapeutic relationship.

Results

While both groups started with similar scores on the C-SRS, the scores between dropouts and completers differed significantly at the end of the treatment period. On average, during therapy, an increase was seen in the scores of completers, and a decrease was seen in the scores of dropouts. While dropouts could not be predicted based on the C-SRS scores, a significant decrease (RCI) in C-SRS scores during the last two sessions occurred more often for dropouts than for completers.

Conclusion

Our findings show that to prevent dropouts, the patient’s judgment of the quality of the therapeutic relationship should be monitored continuously, and decreases discussed with the patient and the group.
The Origins of Intergroup Resource Inequality Influence Children’s Decision to Perpetuate or Rectify Inequality
Authors: An Jing , Yu Jing , Zhu Liqi
Published: November 2020

Previous studies have explored children’s intergroup resource allocation in the context of preexisting intergroup resource inequality. However, resource inequality between social groups often originates from different factors. This study explored the role of the origins of resource inequality on children’s intergroup resource allocations. In experiment 1, when there was no explicit origin of the intergroup inequality, children of different ages mainly allocated resources in an equal way and 5- to 6-year-olds showed ingroup bias. In experiment 2, we examined the influence of different origins of intergroup inequality and found that 5- to 6-year-olds perpetuated intergroup inequality when resource inequality was based on either a structural (regional disparity) or an internal factor (difference in performance). However, 10- to 11-year-olds rectified inequality or allocated equally when intergroup inequality was based on regional disparity and perpetuated resource inequality when intergroup inequality was based on performance difference. The origins of inequality appear to play an important role in children’s intergroup resource allocations, and older children can distinguish different origins of intergroup inequality in resource allocation.
Written Emotional Disclosure Can Promote Athletes’ Mental Health and Performance Readiness During the COVID-19 Pandemic
Authors: Davis Paul A. , Gustafsson Henrik , Callow Nichola , Woodman Tim
Published: November 2020

The widespread effects of the coronavirus disease 2019 (COVID-19) pandemic have negatively impacted upon many athletes’ mental health and increased reports of depression as well as symptoms of anxiety. Disruptions to training and competition schedules can induce athletes’ emotional distress, while concomitant government-imposed restrictions (e.g., social isolation, quarantines) reduce the availability of athletes’ social and emotional support. Written Emotional Disclosure (WED) has been used extensively in a variety of settings with diverse populations as a means to promote emotional processing. The expressive writing protocol has been used to a limited extent in the context of sport and predominantly in support of athletes’ emotional processing during injury rehabilitation. We propose that WED offers an evidence-based treatment that can promote athletes’ mental health and support their return to competition. Research exploring the efficacy of the expressive writing protocol highlights a number of theoretical models underpinning the positive effects of WED; we outline how each of these potential mechanisms can address the multidimensional complexity of the challenging circumstances arising from the COVID-19 pandemic (e.g., loss of earnings, returning to training and competition). Considerations and strategies for using WED to support athletes during the COVID-19 pandemic are presented.
Pilot Study of Trans-oral Robotic-Assisted Needle Direct Tracheostomy Puncture in Patients Requiring Prolonged Mechanical Ventilation
Authors: Xiao Xiao , Poon Howard , Lim Chwee Ming , Meng Max Q.-H. , Ren Hongliang
Published: November 2020

COVID-19 can induce severe respiratory problems that need prolonged mechanical ventilation in the intensive care unit. While Open Tracheostomy (OT) is the preferred technique due to the excellent visualization of the surgical field and structures, Percutaneous Tracheostomy (PT) has proven to be a feasible minimally invasive alternative. However, PT's limitation relates to the inability to precisely enter the cervical trachea at the exact spot since the puncture is often performed based on crude estimation from anatomical laryngeal surface landmarks. Besides, there is no absolute control of the trajectory and force required to make the percutaneous puncture into the trachea, resulting in inadvertent injury to the cricoid ring, cervical esophagus, and vessels in the neck. Therefore, we hypothesize that a flexible mini-robotic system, incorporating the robotic needling technology, can overcome these challenges by allowing the trans-oral robotic instrument of the cervical trachea. This approach promises to improve current PT technology by making the initial trachea puncture from an “inside-out” approach, rather than an “outside-in” manner, fraught with several technical uncertainties.
Revealing Causal Controls of Storage-Streamflow Relationships With a Data-Centric Bayesian Framework Combining Machine Learning and Process-Based Modeling
Authors: Tsai Wen-Ping , Fang Kuai , Ji Xinye , Lawson Kathryn , Shen Chaopeng
Published: November 2020

Some machine learning (ML) methods such as classification trees are useful tools to generate hypotheses about how hydrologic systems function. However, data limitations dictate that ML alone often cannot differentiate between causal and associative relationships. For example, previous ML analysis suggested that soil thickness is the key physiographic factor determining the storage-streamflow correlations in the eastern US. This conclusion is not robust, especially if data are perturbed, and there were alternative, competing explanations including soil texture and terrain slope. However, typical causal analysis based on process-based models (PBMs) is inefficient and susceptible to human bias. Here we demonstrate a more efficient and objective analysis procedure where ML is first applied to generate data-consistent hypotheses, and then a PBM is invoked to verify these hypotheses. We employed a surface-subsurface processes model and conducted perturbation experiments to implement these competing hypotheses and assess the impacts of the changes. The experimental results strongly support the soil thickness hypothesis as opposed to the terrain slope and soil texture ones, which are co-varying and coincidental factors. Thicker soil permits larger saturation excess and longer system memory that carries wet season water storage to influence dry season baseflows. We further suggest this analysis could be formulated into a data-centric Bayesian framework. This study demonstrates that PBM present indispensable value for problems that ML cannot solve alone, and is meant to encourage more synergies between ML and PBM in the future.
Evaluation of the Low Inclusion of Full-Fatted Hermetia illucens Larvae Meal for Layer Chickens: Growth Performance, Nutrient Digestibility, and Gut Health
Authors: Chu Xiaohua , Li Mengmeng , Wang Guiying , Wang Kuiming , Shang Rongsheng , Wang Ziyu , Li Lusheng
Published: November 2020

Substitution of feed protein source with defatted black soldier fly larvae meal (BSFM) has been evaluated intensively in poultry, but information about full-fatted BSFM is still very limited. The aim of the present study was to investigate the effect of dietary low inclusion of full-fatted BSFM on the growth performance, plasma antioxidant ability, nutrient digestibility, and gut health of layer chickens during 1–42 days of age. A total of 480 female 1-day-old Hy-Line Brown chickens were divided into four dietary treatments, with the inclusion of 0, 3, 6, and 9% of full-fatted BSFM. Each treatment included six replicates and 20 birds per replicate. As dietary full-fatted BSFM inclusion levels increased, there was a quadratic increase in final weight and average daily gain and a quadratic decrease in feed/gain ratio. Dietary full-fatted BSFM inclusion levels increased the digestibility of crude protein and ether extract quadratically as well as ileum mucosal sIgA concentration linearly, but these had no effect on intestinal morphology. Additionally, an increase in dietary full-fatted BSFM inclusion levels resulted in a linear increase in glutathione peroxidase and total superoxide dismutase activities and a linear decrease in malondialdehyde content in plasma. The encouraging results of the improvement of growth performance, nutrient digestibility, antioxidant ability, and gut health parameters suggested that partially full-fatted BSFM inclusion can be suitable protein ingredients for layer chickens' diets at the starter period.
Improved Source Characteristics of a Handclap for Acoustic Measurements: Utilization of a Leather Glove
Authors: de Vos Rick , Papadakis Nikolaos M. , Stavroulakis Georgios E.
Published: November 2020

A handclap is a convenient and easily available source for room acoustic measurements. If used correctly (e.g., application of optimal hand configuration) it can provide usable results for the measurement of acoustic parameters, within an expected deviation. Its biggest drawbacks are the low sound pressure level (especially in the low frequency range) as well as its low repeatability. With this in mind, this paper explores the idea of testing a handclap with a glove in order to assess the effect on its source characteristics. For this purpose, measurements were performed with 12 participants wearing leather gloves. Sound levels were compared with simple handclaps without gloves, and between grouped results (overall A-weighted SPL, octave bands, 1/3 octave bands). Measurements were also performed several times to evaluate the effect on repeatability. Results indicate that the use of leather gloves can increase the sound levels of a handclap by 10 dB and 15 dB in the low frequency ranges (63 Hz and 125 Hz octave bands, respectively). Handclaps with leather gloves also point toward improved repeatability, particularly in the low-frequency part of the frequency spectrum. In conclusion, compared to simple handclaps without gloves, evidence from this study supports the concept that handclaps with leather gloves can be used in engineering practices for improved room acoustic measurements of room impulse response.
Active Fault-Tolerant Control Strategy for More Electric Aircraft under Actuation System Failure
Authors: Sun Xiaozhe , Wang Xingjian , Zhou Zhiyuan , Zhou Zhihan
Published: November 2020

The aircraft hydraulic system is very important for the actuation system and its failure has led to a number of catastrophic accidents in the past few years. The reasons for hydraulic loss can be leakage, blockage, and structural damage. Fortunately, the development of more electric aircraft (MEA) provides a new means of solving this difficult problem. This paper designs an active fault tolerant control (AFTC) method for MEA suffering from total hydraulic loss and actuation system failure. Two different kinds of scenarios are considered: leakage/blockage and vertical tail damage. With the application of the dissimilar redundant actuation system (DRAS) in MEA, a switching mechanism can be used to change the hydraulic actuation (HA) system into an electro-hydrostatic actuation (EHA) system when the whole hydraulic system fails. Taking account of the gap between HA and EHA, a degraded model is built. As for vertical tail damage, engine differential thrust control is adopted to help regain lateral-directional stability. The engine thrust dynamics are modeled and the mapping relationship between engine differential thrust and rudder deflection is formulated. Moreover, model reference control (MRC) and linear quadratic regulator (LQR) are used to design the AFTC method. Comparative simulation with the NASA generic transportation model (GTM) is carried out to prove the proposed strategy.
Factors Determining Farmers’ Access to and Sources of Credit: Evidence from the Rain-Fed Zone of Pakistan
Authors: Ullah Ayat , Mahmood Nasir , Zeb Alam , Kächele Harald
Published: November 2020

This study investigates the factors that affect farmers’ access to agricultural credit and its role in adopting improved agricultural technologies in the rain-fed zone of Khyber Pakhtunkhwa (KP), Pakistan. Using logistic models, we assess and compare the relative role of farmers’ socioeconomic attributes in their access to credit and adoption strategies. The results indicate a moderate positive association between farmers’ access to agricultural credit and their adoption of improved agricultural technologies. The binary logit model’s results indicate that farmers with a large-sized farm, high farm income, better access to information, and large physical asset ownership showed a positive influence on credit access. However, farming experience showed a negative effect on farmers’ access to agricultural credit. Regarding farmers’ credit sources, this study found that asset-rich farmers with more farming experience and better access to information relied more on banks than on input providers and informal credit sources. Similarly, older farmers with more education, larger farm sizes and high farm income were more likely to have borrowed from input providers than banks. We conclude that the role of the effective provision of information on credit and agricultural technology is imperative and requires separate policies that are specifically aimed at different groups of farmers with different socioeconomic and farm-related characteristics.
Modification of the TOMCAST Model with Aerobiological Data for Management of Potato Early Blight
Authors: Meno Laura , Escuredo Olga , Rodríguez-Flores Maria Shantal , Seijo Maria Carmen
Published: November 2020

The present study focuses on establishing thresholds of weather variables for predict early blight in potato crops. For this, the TOMCAST model was adjusted using weather variables and Alternaria conidia levels (mainly A. solani and A. alternata) during six growing seasons in A Limia (Northwest Spain). TOMCAST for the effective management of early blight considers leaf wetness and air temperature to calculate daily severity values (DSVs). Spearman correlations between temperature (minimum and average), mean temperature during leaf wetness period and Alternaria concentration showed the highest positive significant coefficients (0.386, 0.230 and 0.372, respectively; p < 0.01). Specifically, Alternaria levels higher than 50 spores/m3 were found the days with air mean temperature above 18 °C, more than 7 h of leaf wetness. Leaf wetness was decisive to estimate the concentration of Alternaria, resulting in a significant linear regression model (R2 = 0.41; p < 0.001). TOMCAST was adapted to the area, considering 10 °C the minimum threshold for the mean value of temperature during the wet period and 10–15 accumulated disease severity values (DSV). Using TOMCAST, it was possible to predict the first Alternaria peak in most of potato growing seasons. Combining aerobiological and meteorological data to control fungal diseases during crops are a useful tool for sustainable agriculture.