bluuebunny/crossref_metadata_2025 huggingface dataset:
Semi-Supervised Interior Decoration Style Classification with Contrastive Mutual Learning
Authors: Guo Lichun , Zeng Hao , Shi Xun , Xu Qing , Shi Jinhui , Bai Kui , Liang Shuang , Hang Wenlong
Published: September 2024

Precisely identifying interior decoration styles holds substantial significance in directing interior decoration practices. Nevertheless, constructing accurate models for the automatic classification of interior decoration styles remains challenging due to the scarcity of expert annotations. To address this problem, we propose a novel pseudo-label-guided contrastive mutual learning framework (PCML) for semi-supervised interior decoration style classification by harnessing large amounts of unlabeled data. Specifically, PCML introduces two distinct subnetworks and selectively utilizes the diversified pseudo-labels generated by each for mutual supervision, thereby mitigating the issue of confirmation bias. For labeled images, the inconsistent pseudo-labels generated by the two subnetworks are employed to identify images that are prone to misclassification. We then devise an inconsistency-aware relearning (ICR) regularization model to perform a review training process. For unlabeled images, we introduce a class-aware contrastive learning (CCL) regularization to learn their discriminative feature representations using the corresponding pseudo-labels. Since the use of distinct subnetworks reduces the risk of both models producing identical erroneous pseudo-labels, CCL can reduce the possibility of noise data sampling to enhance the effectiveness of contrastive learning. The performance of PCML is evaluated on five interior decoration style image datasets. For the average AUC, accuracy, sensitivity, specificity, precision, and F1 scores, PCML obtains improvements of 1.67%, 1.72%, 3.65%, 1.0%, 4.61%, and 4.66% in comparison with the state-of-the-art method, demonstrating the superiority of our method.
Shannon’s Sampling Theorem for Set-Valued Functions with an Application
Authors: Yılmaz Yılmaz , Erdoğan Bağdagül Kartal , Levent Halise
Published: September 2024

In this study, we defined a kind of Fourier expansion of set-valued square-integrable functions. In fact, we have seen that the classical Fourier basis also constitutes a basis for the Hilbert quasilinear space L2(−π,π,Ω(C)) of Ω(C)-valued square-integrable functions, where Ω(C) is the class of all compact subsets of complex numbers. Furthermore, we defined the quasi-Paley–Wiener space, QPW, using the Fourier transform defined for set-valued functions and thus we showed that the sequence sinc.−kk∈Z form also a basis for QPW. We call this result Shannon’s sampling theorem for set-valued functions. Finally, we gave an application based on this theorem.
Computational Modeling of Sodium-Ion-Channel-Based Glucose Sensing Biophysics to Study Cardiac Pacemaker Action Potential
Authors: Mahapatra Chitaranjan , Shanmugam Kirubanandan , Rusho Maher Ali
Published: September 2024

Elevated blood glucose levels, known as hyperglycemia, play a significant role in sudden cardiac arrest, often resulting in sudden cardiac death, particularly among those with diabetes. Understanding the internal mechanisms has been a challenge for healthcare professionals, leading many research groups to investigate the relationship between blood glucose levels and cardiac electrical activity. Our hypothesis suggests that glucose-sensing biophysics mechanisms in cardiac tissue could clarify this connection. To explore this, we adapted a single-compartment computational model of the human pacemaker action potential. We incorporated glucose-sensing mechanisms with voltage-gated sodium ion channels using ordinary differential equations. Parameters for the model were based on existing experimental studies to mimic the impact of glucose levels on pacemaker action potential firing. Simulations using voltage clamp and current clamp techniques showed that elevated glucose levels decreased sodium ion channel currents, leading to a reduction in the pacemaker action potential frequency. In summary, our mathematical model provides a cellular-level understanding of how high glucose levels can lead to bradycardia and sudden cardiac death.
The Neutrophil-to-Lymphocyte Ratio in Patients with Spinal Cord Injury: A Narrative Review Study
Authors: Naseri Alavi Seyed Ahmad , Habibi Mohammad Amin , Naseri Alavi Seyed Hamed , Zamani Mahsa , Kobets Andrew J.
Published: September 2024

Background and Objectives: Traumatic spinal cord injury (SCI) is a devastating condition that occurs in two phases: primary and secondary injury. These phases contribute to changes in blood vessels and the influx of inflammatory cells such as neutrophils and lymphocytes. The biomarker known as the neutrophil-to-lymphocyte ratio (NLR) has been suggested as being highly valuable in predicting outcomes for patients with traumatic brain injury, acute ischemic stroke, and traumatic spinal cord injury. Therefore, this review study aims to investigate the prognostic value of the NLR in predicting outcomes for patients with SCI. Materials and Methods: A thorough review of relevant articles was conducted using Mesh keywords in Medline via Embase, PubMed, Google Scholar, and Scopus from 2000 to 2023. The search was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist. After reviewing the articles and applying inclusion and exclusion criteria, only relevant articles were included in the study. Results: In the initial search, 41 papers were identified. After applying exclusion criteria, only three clinical studies remained for review. It is still debatable whether the NLR can serve as a cost-effective, readily available, and independent predictive factor for both mortality and recovery outcomes in patients with traumatic spinal cord injuries. Conclusions: Our study demonstrates that NLR, a readily available and inexpensive marker, can serve as an independent predictor of both mortality and recovery outcomes in patients with traumatic spinal cord injury. To reach a conclusive decision, additional data are required.
White Tea Consumption Alleviates Anthropometric and Metabolic Parameters in Obese Patients
Authors: Akyildiz Kerimali , Yilmaz Adnan , Avci Ugur , Toraman Merve Nur , Yazici Zihni Acar
Published: September 2024

Background and Objectives: Obesity and related disorders are an increasing global health problem. Achieving and maintaining long-term weight loss through lifestyle changes and/or pharmacological interventions have not met expectations. Dietary supplements and alternative treatments have also shown limited effectiveness in this regard. The consumption of green tea in general has been shown to benefit obese patients, with effects attributed to caffeine, catechins, polyphenols and other components. However, the potential of white tea to prevent and treat the negative effects of obesity has not been addressed so far. In this study, the effect of white tea (WT) consumption in obese individuals was anthropometrically and biochemically investigated. Materials and Methods: Based on anthropometric and biochemical assessments, the patients were assigned to the control, orlistat, metformin and WT groups. Patients were given a diet and exercise program and one of either orlistat, metformin or WT for 12 weeks. At the end of the 12th week, the anthropometric and biochemical measurements were reassessed. Results: Body weight, waist circumference and BMI parameters decreased significantly in all groups. TNF-α, IL-6, IL-1β and MMP-9 levels decreased significantly in the WT group. In addition, contrary to a significant elevation in HDL-C, the serum cholesterol, LDL-C and TG levels decreased significantly. Furthermore, leptin, ghrelin and asprosin levels decreased significantly. Serum glucose levels decreased significantly in all groups except for the control. In the WT group, while there was a significant decrease in the levels of serum PL MDA and 8-OHdG, the opposite was true for GSH. Conclusions: The oral consumption of WT, its availability and its potency in obesity treatment and prevention pave the way for further delineation of the mechanisms of actions of its bioactive compounds at the cellular and endocrinological levels.
Adrenal Insufficiency in Patients with Beta Thalassemia: A Meta-Analysis
Authors: Savvidis Christos , Ragia Dimitra , Delicou Sophia , Xydaki Aikaterini , Rizzo Manfredi , Ilias Ioannis
Published: September 2024

Background and Objectives: Adrenal insufficiency (AI) can be a significant concern in patients with transfusion-dependent homozygous beta thalassemia (bThal) due to the chronic disease burden and frequent blood transfusions that these patients require. The prevalence of AI in this population remains unclear, with studies often lacking control groups for comparison. This meta-analysis aimed to estimate the proportion of patients with transfusion-dependent bThal who exhibit evidence of AI. Materials and Methods: A systematic review following PRISMA guidelines identified 19 studies for analysis. Results: Despite the variability in the diagnostic methods used to ascertain AI, the meta-analysis revealed that approximately one-third of patients had evidence of AI, with the prevalence rising to 50% in studies focused on adults with bThal. Conclusions: These findings suggest an increased risk of AI in patients with bThal compared to the general population. Clinicians should consider tailored management strategies, including glucocorticoid coverage during surgical procedures, to mitigate the risk of adrenal crises in this vulnerable patient group. Further research is needed to optimize adrenal surveillance and management in patients with bThal.
The Influence of the Hot-Rolling Temperature on the Microstructure and Mechanical Properties of Ti-Nb Microalloyed 21%Cr Ferritic Stainless Steel
Authors: Meng Yahui , Sheng Jie , Ma Zongwen , Gao Yang , Tuo Lixu , Duan Hongyan , Man Kun
Published: September 2024

Microalloying and heat treatment are essential processing techniques for ferritic stainless steel (FSS). Three different compositions of 21%Cr FSS with 0.28Ti, 0.21Ti + 0.05Nb, and 1.05Ti + 0.17Nb were prepared. The interaction effects of the Nb and Ti contents and hot-rolling annealing on the microstructure, mechanical properties, and precipitate phases of FSS were studied. The microstructure, crystal structure, and precipitation phase of steel at 930, 980, and 1030 °C with Ti-Nb microalloying were investigated using an optical microscope (OM), X-ray diffractometer (XRD), and scanning electron microscope (SEM). The room-temperature tensile properties, surface roughness, and hardness were tested separately. This study found that the composite addition of Ti and Nb had a dual effect of fine-grain strengthening and precipitation strengthening. The 1.05Ti + 0.17Nb steel specimen had a moderate grain size and the best uniformity after hot rolling at 980 °C. The tensile strength and elongation were 454 MPa and 34.2%, which achieved an optimal balance between strength and plasticity.
Phase-Field Simulation and Dendrite Evolution Analysis of Solidification Process for Cu-W Alloy Contact Materials under Arc Ablation
Authors: Ren Hanwen , Mu Jian , Zhao Siyang , Li Junke , Yang Yateng , Han Zhiyun , Xing Zexi , Li Qingmin
Published: September 2024

Cu-W alloys are widely used in high-voltage circuit breaker contacts due to their high resistance to arc ablation, but few studies have analyzed the microstructure of Cu-W alloys under arc ablation. This study applied a phase-field model based on the phase-field model developed by Karma and co-workers to the evolution of dendrite growth in the solidification process of Cu-W alloy under arc ablation. The process of columnar dendrite evolution during solidification was simulated, and the effect of the supercooling degree and anisotropic strength on the morphology of the dendrites during solidification was analyzed. The results show that the solid–liquid interface becomes unstable with the release of latent heat, and competitive growth between dendrites occurs with a large amount of solute discharge. In addition, when the supercooling degree is 289 K, the interface is located at a lower height of only 15 μm, and the growth rate is slow. At high anisotropy, the side branches of the dendrites are more fully developed and tertiary dendritic arms appear, leading to a decrease in the alloy’s relative density and poorer ablation resistance. In contrast, the main dendrites are more developed under high supercooling, which improves the density and ablation resistance of the material. The results in this paper may provide a novel way to study the microstructure evolution and material property changes in Cu-W alloys under the high temperature of the arc for high-voltage circuit breaker contacts.
Ultra-High-Performance Liquid Chromatography–High-Definition Mass Spectrometry-Based Metabolomics to Reveal the Potential Anti-Arthritic Effects of Illicium verum in Cultured Fibroblast-like Synoviocytes Derived from Rheumatoid Arthritis
Authors: Qin Mingzhen , Chen Lu , Hou Xiaoli , Wu Wuwei , Liu Yu , Pan Yu , Zhang Mengli , Tan Zhien , Huang Danna
Published: September 2024

Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. The fruits of Illicium verum, which is a medicinal and edible resource, have been shown to have anti-inflammatory properties. Methods: In this study, we investigated the effects of I. verum extracts (IVEs) on human RA fibroblasts-like synoviocytes (RA-FLS) by using a sensitive and selective ultra-high-performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) method. We subsequently analyzed the metabolites produced after the incubation of cultured RA-FLS with IVEs. Results: IVEs inhibited the proliferation and suppressed the migration of RA-FLS, and reduced the levels of inflammatory factors including TNF-α and IL-6. Twenty differential metabolites responsible for the effects of IVEs were screened and annotated based on the UPLC-HDMS data by using a cell metabolomics approach. Discussion: Our findings suggest that treating RA-FLS with IVEs can regulate lipid and amino acid metabolism, indicating that this extract has the potential to modify the metabolic pathways that cause inflammation in RA. Conclusions: This might lead to novel therapeutic strategies for managing patients with RA.
Development of Anemometer Based on Inertial Sensor
Authors: Rocha Álvaro B. , Fernandes Eisenhawer de M. , Souto Joyce I. V. , Gomez Ricardo S. , Delgado João M. P. Q. , Lima Felipe S. , Alves Railson M. N. , Bezerra André L. D. , Lima Antonio G. B.
Published: September 2024

The current article elucidates a study centered on the development of an anemometer leveraging an inertial sensor for wind speed measurement in the northeast region of Brazil, focusing on renewable energy generation. The study encompassed a series of experiments aimed at calibrating the anemometer, analyzing the noise generated by the inertial sensor, and scrutinizing the data acquired during wind speed measurement. The calibration process unfolded in three stages: initial noise analysis, subsequent inertial data analysis, and the derivation of calibration curves. The first two stages involved experiments conducted at an average sampling rate of 10 Hz. Simultaneously, the third stage incorporated data collected over a 1 h duration while maintaining the same sampling rate. The outcomes underscore the suitability of the anemometer based on an inertial sensor for wind energy systems and diverse applications. While the wind readings from the prototype exhibit considerable fluctuations, a three-length moving average filter is applied to the prototype’s output to mitigate these fluctuations. The calibration surface was established using observational data, and the resultant surface is detailed. Data analysis assumes paramount significance in wind speed measurement, and the K-NN algorithm demonstrated superior efficacy in estimating the correspondence between measured and control data.
Challenges in Diagnosis of COVID-19 Pneumonia under Ocrelizumab and De-Risking Strategies in Multiple Sclerosis—The Elephant Is (Still) in the Room
Authors: Mariottini Alice , Lotti Antonio , Damato Valentina , Massacesi Luca
Published: September 2024

Severe SARS-CoV-2 infections may still be observed in people bearing risk factors, such as the use of anti-CD20 monoclonal antibodies (mAbs), which are adopted in several autoimmune disorders including multiple sclerosis (MS). COVID-19 diagnosis is routinely based on nasopharyngeal swab testing, but suboptimal sensitivity for SARS-CoV-2 detection compared to bronchoalveolar lavage (BAL) may lead to misdiagnosis in some cases. Such diagnostic issues were described in a few MS patients receiving anti-CD20 mAbs, including middle-aged people and lacking information on subsequent MS therapeutic management, a debated topic as no evidence-based guidance on de-risking strategies is currently available. Here, we report the case of a young MS patient who developed severe COVID-19 pneumonia under treatment with the anti-CD20 mAb ocrelizumab, and who was finally diagnosed with SARS-CoV-2 by BAL despite repeatedly negative nasopharyngeal swabs. Ocrelizumab was then discontinued, and treatment with a sphingosine-1 phosphate receptor modulator was started, followed by maintenance of clinical and radiological MS stability. Challenges in diagnosing COVID-19 pneumonia in people without risk factors other than immunomodulatory treatment are hence discussed, as well as potential strategies for de-risking MS therapies. The latter topic is increasingly debated based on raising concerns for potential long-term safety issues of high-efficacy treatments, including anti-CD20 mAbs.
Nonprecious Single Atom Catalyst for Methane Pyrolysis
Authors: Helsel Naomi , Chowdhury Sanchari , Choudhury Pabitra
Published: September 2024

The development of a suitable catalytic system for methane pyrolysis reactions requires a detailed investigation of the activation energy of C-H bonds on catalysts, as well as their stability against sintering and coke formation. In this work, both single-metal Ni atoms and small clusters of Ni atoms deposited on titanium nitride (TiN) plasmonic nanoparticles were characterized for the C-H bond activation of a methane pyrolysis reaction using ab initio spin-polarized density functional theory (DFT) calculations. The present work shows the complete reaction pathway, including energy barriers for C-H bond activation and dehydrogenated fragments, during the methane pyrolysis reaction on catalytic systems. Interestingly, the C-H bond activation barriers were low for both Ni single-atom and Ni-clusters, showing the energy barriers of ~1.10 eV and ~0.88 eV, respectively. Additionally, single-atom Ni-TiN showed weaker binding to adsorbates, and a net endothermic reaction pathway indicated that the single-atom Ni-TiN was expected to resist coke formation on its surface. However, these Ni single-atom catalysts can sinter, aggregate into a small cluster, and form a coke layer from the highly exothermic reaction pathway that the cluster takes despite the facile reaction pathway.
Discovery of Cinnamic Acid Derivatives as Potent Anti-H. pylori Agents
Authors: Li Yonglian , Zhao Kun , Wu Zhidi , Zheng Yujun , Yu Jialin , Wu Sikun , Wong Vincent Kam Wai , Chen Min , Liu Wenfeng , Zhao Suqing
Published: September 2024

Antibiotics are currently used for the treatment of Helicobacter pylori (H. pylori), which is confirmed to be the major cause of gastric disorders. However, the long-term consumption of antibiotics has already caused antibiotic resistance and side effects in vivo. Therefore, there is an emerging need for searching for safe and effective anti-H. pylori agents. Inspired by the excellent bioactivities of cinnamic acid, a series of cinnamic acid derivatives (compounds 1–30) were synthesized and determined for H. pylori inhibition. The initial screening revealed that compound 23, a 2,4-dinitro cinnamic acid derivative containing 4-methoxyphenol, showed excellent H. pylori inhibition with an MIC value of 4 μM. Further studies indicated that compound 23 showed anti-bacterial activity and had a bactericidal effect on H. pylori due to the destruction of the bacterial structure. Molecular docking analysis revealed that the 2,4-dinitro groups in cinnamic acid moiety formed hydrogen bonding with amino acid residues in an active pocket of H. pylori protein. Interestingly, the ester moiety fitted into the hydrophobic pocket, attaining additional stability to compound 23. Above all, the present study reveals that compound 23 could be considered a promising anti-H. pylori agent to treat H. pylori causing gastritis.
Stress-Relieving Effects of Green Tea Depend on the Ratio of Its Special Ingredients and the Infusion Conditions
Authors: Unno Keiko , Taguchi Kyoko , Matsuda Tomoko , Nakamura Yoriyuki
Published: September 2024

Theanine, an amino acid unique to tea leaves, has been reported to exhibit stress-relieving effects. However, the stress-relieving effects of theanine (T) are greatly inhibited by caffeine (C) and epigallocatechin gallate (E), the main components of green tea, while being enhanced by arginine (A). Animal and clinical studies using matcha tea have shown that it can alleviate stress if the molar ratio of C and E against T and A (CE/TA) is less than 2. In general, the CE/TA ratio in tea leaves is reflected by the CE/TA ratio in the infused solution; however, it is not clear what infusion conditions would be expected to ensure a stress-relieving effect. In this study, to examine the stress-relieving effect of green-tea-infused solutions, the CE/TA ratio was measured under different infusion conditions. Furthermore, a study using a model solution consisting of C, E, T, and A revealed that a CE/TA ratio of at least less than 2 and a T content greater than 0.23 mM were required for stress reduction. Based on these results, we identified tea leaves and their infusion conditions that can be expected to reduce stress.
Surfactant-Assisted Regulation of WS2/Tourmaline Microstructures for Excellent Photocatalytic Performance
Authors: Wang Xianku , Cui Kaibin , Zhao Yuqin , Hao Ming , Bian Liang , Wang Mingming , Wang Fei
Published: September 2024

The controllable electrical and optical properties of two-dimensional tungsten disulfide (WS2) attracted much attention in photocatalysis, but commercial development has been severely restricted by their restacking properties. Surfactant-assisted synthesis techniques can be considered as an effective option to break this bottleneck. In this work, the effect of surfactants including sodium dodecylbenzene sulfonate (SDBS), hexadecyltrimethylammonium bromide (CTAB), and polyvinylpyrrolidone (PVP) on the microstructure of WS2/tourmaline composites prepared by coupled hydrothermal and calcination methods was explored. The WS2 nanosheets were uniformly deposited on the tourmaline surface with the assistance of 1.0 mmol/L SDBS. Meanwhile, WS2/Tour-SDBS exhibited the highest rhodamine B (RhB) degradation activity, which was 1.8 and 2.3 times higher than that of photocatalysts prepared with CTAB and PVP under the same conditions, respectively. This study provides a new tactic for the fabrication of high-performance WS2-based composites.
Cosmetically Applicable Soluble Agonists for Toll-like Receptor 2 Produced by Fermentation of Asparagus Extract Supplemented with Skimmed Milk Using Lactobacillus delbrueckii subsp. lactis TL24 Consist of Molecules Larger than 100 kDa and Can Be Stabilized by Lyophilization with Dextrin
Authors: Komatsu Yasuhiko , Matsunaga Kanako
Published: September 2024

Cosmetically applicable soluble agonists for Toll-like receptor 2 (TLR2), which can strengthen skin barrier function, were produced by fermentation of asparagus (Asparagus officinalis L.) extract supplemented with skimmed milk using Lactobacillus delbrueckii subsp. lactis TL24. Their molecular size was estimated to be >100 kDa. Their TLR2-stimulating activity was stable over 1 year at 4 °C, but it decreased by more than 95% within 10 and 4 months at 25 °C and 40 °C, respectively. The possibility of stabilization of TLR2-stimulating activity by powdering was tested, and we found that lyophilization with 10% or a higher amount of dextrin could stabilize the activity even at 40 °C. The powdered fermented product dose-dependently stimulated TLR2. It augmented the formation of tight junctions in normal human keratinocytes, as detected by fluorescence staining of occludin and ZO-1, whereas their protein and gene expression levels did not increase, suggesting that a change in subcellular localization of these proteins without significant changes in their amounts might be responsible. The powder nature has some benefits over the aqueous, besides stability, e.g., it can be dissolved just before application, allowing fresh material to be used each time, and it may widen a range of cosmetic applications in non-aqueous types of cosmetics.
A Comparative Review of Graphene and MXene-Based Composites towards Gas Sensing
Authors: Vaishag Pushpalatha Vijayakumar , Noh Jin-Seo
Published: September 2024

Graphene and MXenes have emerged as promising materials for gas sensing applications due to their unique properties and superior performance. This review focuses on the fabrication techniques, applications, and sensing mechanisms of graphene and MXene-based composites in gas sensing. Gas sensors are crucial in various fields, including healthcare, environmental monitoring, and industrial safety, for detecting and monitoring gases such as hydrogen sulfide (H2S), nitrogen dioxide (NO2), and ammonia (NH3). Conventional metal oxides like tin oxide (SnO2) and zinc oxide (ZnO) have been widely used, but graphene and MXenes offer enhanced sensitivity, selectivity, and response times. Graphene-based sensors can detect low concentrations of gases like H2S and NH3, while functionalization can improve their gas-specific selectivity. MXenes, a new class of two-dimensional materials, exhibit high electrical conductivity and tunable surface chemistry, making them suitable for selective and sensitive detection of various gases, including VOCs and humidity. Other materials, such as metal-organic frameworks (MOFs) and conducting polymers, have also shown potential in gas sensing applications, which may be doped into graphene and MXene layers to improve the sensitivity of the sensors.
Efficacy, Safety, and Tolerability of a Very Low-Calorie Ketogenic Diet in Women with Obesity and Symptomatic Knee Osteoarthritis: A Pilot Interventional Study
Authors: Ciaffi Jacopo , Mancarella Luana , Pederzani Giulia , Lisi Lucia , Brusi Veronica , Pignatti Federica , Ricci Susanna , Vitali Giorgia , Faldini Cesare , Ursini Francesco
Published: September 2024

Background/Objectives: Obesity is a major risk factor for knee osteoarthritis (OA), and weight loss is crucial for its management. This pilot study explores the effects of a Very Low-Calorie Ketogenic Diet (VLCKD) in women with obesity and symptomatic knee OA. Methods: Women with symptomatic knee OA and obesity, defined as a body mass index (BMI) ≥ 30 kg/m2, were eligible for the VLCKD protocol. The intervention included a ketogenic phase from baseline (T0) to the 8th week (T8), followed by a progressive reintroduction of carbohydrates over the next 12 weeks, ending at the 20th week (T20). Body mass index (BMI), the Western Ontario and McMaster Universities (WOMAC) Osteoarthritis Index, the EuroQol 5D (EQ-5D), and the 36-item Short Form Health Survey (SF-36) were assessed at all time points. Generalized estimating equations were used to analyze the association between BMI and patient-reported outcomes across the study period. Results: Twenty participants started the study, but four discontinued the intervention, with two of these being due to adverse effects. The mean age of the 16 patients who completed the 20-week program was 57.3 ± 5.5 years, and their mean BMI was 40.0 ± 4.8 kg/m2. The mean BMI significantly decreased to 37.5 ± 4.5 at T4, 36.3 ± 4.6 at T8, and 34.8 ± 4.8 at T20 (all p < 0.001 compared to baseline). The total WOMAC score improved from a mean of 43.6 ± 16.9 at T0 to 30.2 ± 12.8 at T4 (p = 0.005) and further to 24.7 ± 10.6 at T8 (p = 0.001) and to 24.8 ± 15.9 at T20 (p = 0.005). The reduction in BMI was significantly correlated with the improvements in WOMAC, EQ-5D, and SF-36 over time. No major adverse effects were observed. Conclusions: A 20-week VLCKD in women with obesity and knee OA significantly reduced their weight and improved their outcomes, warranting further research. This trial is registered with number NCT05848544 on ClinicalTrials.gov.
Cytotoxic Activity of Vancomycin-Resistant Enterococci Isolated from Hospitalised Patients
Authors: Szczuka Ewa , Rolnicka Dominika , Wesołowska Maria
Published: September 2024

Vancomycin-resistant enterococci (VRE) are considered one of the main nosocomial pathogens due to their increasing antibiotic resistance and ability to cause life-threatening infections in humans. This study included VRE isolates obtained from various specimens including urine, blood, faeces, wounds, sputum, and oral cavity wash. Of the 37 strains, 30 (81.1%) and 7 (18.9%) were identified by MALDI TOF as Enterococcus faecium and Enterococcus faecalis, respectively. The clinical vancomycin-resistant enterococci exhibited multi-drug resistance (MDR). Apart from vancomycin, the enterococci exhibited resistance to penicillins (89.1 to 100%), fluoroquinolones (100%), rifampicin (86.5%), tetracycline (27%), aminoglycosides (56.8 to 86.5%), quinupristin–dalfopristin (35.1%), and chloramphenicol (10.8%). Moreover, resistance to linezolid and tigecycline emerged among the tested vancomycin-resistant enterococci. The analysis of aminoglycoside modifying enzyme (AME) genes showed the presence of bifunctional aac(6′)-Ie-aph(2″)-Ia genes contributed to high-level aminoglycoside resistance (HLAR) in the E. faecalis and E. faecium isolates. The other AME gene, i.e., aph(3′)-IIIa, was also found in the VRE isolates. All strains carried the vanA gene. Enterococci from colonised gastrointestinal tracts (1/2.7%) and from infection (6/16.2%) showed cytotoxic activity against the human epithelial cell line HEp-2.
Therapeutic, Clinicopathological, and Molecular Correlates of PRKACA Expression in Gastrointestinal Cancers
Authors: Othaim Ayoub Al , Alasiri Glowi , Alfahed Abdulaziz
Published: September 2024

Background/Objectives: PRKACA alterations have clear diagnostic and biological roles in the fibrolamellar variant of hepatocellular carcinoma and a potential predictive role in that cancer type. However, the roles of PRKACA have not been comprehensively examined in gastric and colorectal cancers (GC and CRC). This study, therefore, sought to investigate the roles of PRKACA expression in GC and CRC. Methods: The clinico-genomic data of 441 GC and 629 CRC cases were analyzed for therapeutic, clinicopathological, and biological correlates using appropriate bioinformatics and statistical tools. Furthermore, the deregulation of PRKACA expression in GC and CRC was investigated using correlative and regression analyses. Results: The results showed that PRKACA expression subsets were enriched for gene targets of chemotherapeutics, tyrosine kinase, and β-adrenergic inhibitors. Moreover, high PRKACA expression was associated with adverse clinicopathological and genomic features of GC and CRC. Gene Ontology Enrichment Analysis also showed that PRKACA-high subsets of the GI cancers were enriched for the biological and molecular functions that are associated with cell motility, invasion, and metastasis but not cell proliferation. Finally, multiple regression analyses identified multiple methylation loci, transcription factors, miRNA species, and PRKACA copy number changes that deregulated PRKACA expression in GC and CRC. Conclusions: This study has identified potential predictive and clinicopathological roles for PRKACA expression in GI cancers and has added to the growing body of knowledge on the deregulation of PRKACA in cancer.
Synthesis and Application of Silver Nanoparticles for Caries Management: A Review
Authors: Yin Iris Xiaoxue , Xu Veena Wenqing , Xu Grace Yuchan , Yu Ollie Yiru , Niu John Yun , Chu Chun Hung
Published: September 2024

Silver nanoparticles have unique physical, chemical, and biological properties that make them attractive for medical applications. They have gained attention in dentistry for their potential use in caries management. This study reviews the different synthesis methods of silver nanoparticles and the application of them for caries management. Silver nanoparticles are tiny silver and are typically less than 100 nanometres in size. They have a high surface area-to-volume ratio, making them highly reactive and allowing them to interact with bacteria and other materials at the molecular level. Silver nanoparticles have low toxicity and biocompatibility. Researchers have employed various methods to synthesise silver nanoparticles, including chemical, physical, and biological methods. By controlling the process, silver nanoparticles have defined sizes, shapes, and surface properties for wide use. Silver nanoparticles exhibit strong antibacterial properties, capable of inhibiting a broad range of bacteria, including antibiotic-resistant strains. They inhibit the growth of cariogenic bacteria, such as Streptococcus mutans. They can disrupt bacterial cell membranes, interfere with enzyme activity, and inhibit bacterial replication. Silver nanoparticles can inhibit biofilm formation, reducing the risk of caries development. Additionally, nano silver fluoride prevents dental caries by promoting tooth remineralisation. They can interact with the tooth structure and enhance the deposition of hydroxyapatite, aiding in repairing early-stage carious lesions. Silver nanoparticles can also be incorporated into dental restorative materials such as composite resins and glass ionomer cements. The incorporation can enhance the material’s antibacterial properties, reducing the risk of secondary caries and improving the longevity of the restoration.
Inhibition of Angiogenesis and Effect on Inflammatory Bowel Disease of Ginsenoside Rg3-Loaded Thermosensitive Hydrogel
Authors: Xie Yiqiong , Ma Ying , Xu Lu , Liu Hongwen , Ge Weihong , Wu Baojuan , Duan Hongjue , Zhang Hongmei , Fu Yuping , Xu Hang , Sun Yuxiang , Han Zhou , Zhu Yun
Published: September 2024

Background: Inflammatory bowel disease (IBD), characterized by chronic inflammation of the digestive tract, involves angiogenesis as a key pathogenic mechanism. Ginsenoside Rg3, derived from the traditional Chinese herb ginseng, is recognized for its anti-angiogenic properties but is limited by low oral bioavailability. This necessitates the development of an alternative delivery system to improve its therapeutic effectiveness. Methods: Pluronic F-127 (F127) and Pluronic F-68 (F68) were used to construct Rg3-loaded thermosensitive hydrogel Gel-Rg3. Meanwhile, a series of physicochemical properties were determined. Then the safety and pharmacological activity of Gel-Rg3 were evaluated in vitro and in vivo using human umbilical vein endothelial cells (HUVECs) and colitis mouse model, in order to initially validate the potential of Gel-Rg3 for the treatment of IBD. Results: We engineered a rectally administrable, thermosensitive Gel-Rg3 hydrogel using F127 and F68, which forms at body temperature, enhancing Rg3’s intestinal retention and slowly releasing the drug. In vitro, Gel-Rg3 demonstrated superior anti-angiogenic activity by inhibiting HUVEC proliferation, migration, and tube formation. It also proved safer and better suited for IBD’s delicate intestinal environment than unformulated Rg3. In vivo assessments confirmed increased intestinal adhesion and anti-angiogenic efficacy. Conclusions: The Gel-Rg3 hydrogel shows promise for IBD therapy by effectively inhibiting angiogenesis via rectal delivery, overcoming Rg3’s bioavailability limitations with improved safety and efficacy. This study provides new inspiration and data support for the design of treatment strategies for IBD.
Using Hybrid MnO2-Au Nanoflowers to Accelerate ROS Scavenging and Wound Healing in Diabetes
Authors: Jiang Ning , Liu Xinwei , Sui Baiyan , Wang Jiale , Liu Xin , Zhang Zun
Published: September 2024

Objectives: Excessive reactive oxygen species (ROS) in diabetic wounds are major contributors to chronic wounds and impaired healing, posing significant challenges in regenerative medicine. Developing innovative drug delivery systems is crucial to address these issues by modifying the adverse microenvironment and promoting effective wound healing. Methods: Herein, we designed a novel drug delivery platform using manganese dioxide nanoflower hybridized gold nanoparticle composites (MnO2-Au) synthesized via a hydrothermal reaction, and investigated the potential of MnO2-Au nanoflowers to relieve the high oxidative stress microenvironment and regulate diabetic wound tissue healing. Results: This hybrid material demonstrated superior catalytic activity compared to MnO2 alone, enabling the rapid decomposition of hydrogen peroxide and a substantial reduction in ROS levels within dermal fibroblasts. The MnO2-Au nanoflowers also facilitated enhanced dermal fibroblast migration and Col-I expression, which are critical for tissue regeneration. Additionally, a hydrogel-based wound dressing incorporating MnO2-Au nanoflowers was developed, showing its potential as an intelligent drug delivery system. This dressing significantly reduced oxidative stress, accelerated wound closure, and improved the quality of neonatal epithelial tissue regeneration in a diabetic rat skin defect model. Conclusions: Our findings underscore the potential of MnO2-Au nanoflower-based drug delivery systems as a promising therapeutic approach for chronic wound healing, particularly in regenerative medicine.
Relationship between Pharmacists’ Emotional Intelligence and Job Performance: A Cross-Sectional Study in Saudi Arabia
Authors: Almogbel Yasser S. , Alsalloum Muath A. , Almadi Rubiaan S. , Almazyad Abdulaziz A. , Garwan Yusuf M. , Alregaibah Razan A.
Published: September 2024

Pharmacists’ job performance is crucial for improving pharmacy services. The purpose of this study was to evaluate the association of emotional intelligence with the job performance of pharmacists in Saudi Arabia. Using social media platforms, we disseminated an online questionnaire to pharmacists licensed to practice in Saudi Arabia between June and July 2022. The questionnaire was filled out by 352 pharmacists. The majority of the participants were women (60.5%) and working as community pharmacists (55.7%). On self-reported emotional intelligence and job performance scales, the respondents scored an average of 5.5 ± 0.9 (out of 7) and 4.0 ± 0.6 (out of 5), respectively. Multiple linear regression analyses revealed that emotional intelligence had a significant relationship with job performance (β = 0.43, p < 0.001). In conclusion, the findings indicated that emotional intelligence may influence the job performance of pharmacists in Saudi Arabia. As the primary objective of every organization is to accomplish the best possible performance, prioritizing emotional intelligence is important. Further research is needed to identify the impact of emotional intelligence on work performance, which could potentially enhance clinical outcomes for patients.
An Improved Sampling and Baiting Method for Phytophthora tropicalis and P. heveae Detection in Macadamia integrifolia
Authors: Ference Christopher M. , Keith Lisa M.
Published: September 2024

Macadamia nuts are, economically, the second most important crop in the state of Hawai’i. A recent decline in yield and acreage has been attributed to insect damage and diseases such as Macadamia Quick Decline (MQD) caused by Phytophthora tropicalis and P. heveae. To develop an improved methodology for the diagnosis and treatment of MQD, investigations were undertaken to better understand the pathosystem of the disease. These investigations included sampling from multiple locations from sectioned trees utilizing two methods of tissue collection and isolations using two baiting techniques. The collection of tissue from the cambium and phloem of trees after scraping away the bark and in locations of recent or current sap exudation using a narrow diameter steel awl proved to be an efficient means for the molecular detection of the MQD pathogens from infected trees exhibiting MQD symptoms. In addition, a more efficient and cost-effective baiting method using apple puree was developed.
Functional UV Blocking and Superhydrophobic Coatings Based on Functionalized CeO2 and Al2O3 Nanoparticles in a Polyurethane Nanocomposite
Authors: Velasco-Soto Miguel Angel , Vázquez-Velázquez Arturo Román , Pérez-García Sergio Alfonso , Bautista-Carrillo Lilia Magdalena , Vorobiev Pavel , Méndez-Reséndiz Abraham , Licea-Jiménez Liliana
Published: September 2024

Water repellency has significant potential in applications like self-cleaning coatings, anti-staining textiles, and electronics. This study introduces a novel nanocomposite system incorporating functionalized Al2O3 and CeO2 nanoparticles within a polyurethane matrix to achieve hydrophobic and UV-blocking properties. The nanoparticles were functionalized using an octadecyl phosphonic acid solution and characterized by FTIR and XPS, confirming non-covalent functionalization. Spin-coated polyurethane coatings with functionalized and non-functionalized Al2O3, CeO2, and binary Al2O3-CeO2 nanoparticles were analyzed. The three-layered Al2O3-CeO2-ODPA binary system achieved a contact angle of 166.4° and 85% transmittance in the visible range. Incorporating this binary functionalized system into a 0.4% w/v polyurethane solution resulted in a nanocomposite with 75% visible transmittance, 60% at 365 nm UV, and a 147.7° contact angle after three layers. These findings suggest that ODPA-functionalized nanoparticles, when combined with a polymer matrix, offer a promising approach to developing advanced hydrophobic and UV-protective coatings with potential applications across various industrial sectors.
Utilization of Forest Residues for Cellulose Extraction from Timber Species in the High Montane Forest of Chimborazo, Ecuador
Authors: Manzano Vela Dennis Renato , Villegas Freire Cristina Nataly , Zabala Vizuete Rolando Fabian , Flores Mancheno Ana Carola
Published: September 2024

The present study explored the extraction of cellulose from forest residues of four timber species, namely Cedrela montana Moritz ex Turcz, Buddleja incana Ruiz & Pav, Vallea stipularis L. f. and Myrsine andina (Mez) Pipoly, in the high montane forest of Chimborazo province, Ecuador, for the sustainable utilization of leaves, branches, and flowers. An alkaline extraction method was used on the residues without the need for prior degreasing. An ANOVA analysis was applied to evaluate significant differences in cellulose extraction yields among the species’ residues. The characterization techniques used were Fourier transform infrared spectroscopy (FTIR) and polarized light optical microscopy, which confirmed the successful extraction of cellulose with characteristics comparable to standard cotton cellulose and other traditional species. The results showed significant variations in cellulose yield among the species, with Vallea stipularis L. f achieving the highest yield of 80.83%. The crystallinity of the samples was clearly evidenced by the polarity of the light in the samples during microscopy, demonstrating that the residues can be a viable and sustainable source of cellulose, contributing to a circular economy and reducing the environmental impact of forest waste.
Numerical Simulation Study on Optimization of Development Parameters of Condensate Gas Reservoirs
Authors: Zhu Kai , Gao Lingjie , Sun Fengrui
Published: September 2024

Due to the retrograde condensation phenomenon in the development process, the fluid phase change is complex, and it becomes particularly difficult to accurately describe the fluid flow characteristics and residual oil and gas distribution characteristics during the development of condensate gas reservoirs. It is difficult to select the development program and subsequent dynamic adjustment for the efficient, reasonable, and sustainable development of condensate gas reservoirs. In this paper, the phase characteristics of condensate gas reservoirs are clarified; the basic fluid model is created by using computer modeling, using Win-Prop; and in view of the characteristics of the target condensate gas reservoirs, the CMG (Computer Modeling Group) numerical simulation method is applied to study the effects of six factors, the thickness of the reservoir, permeability, porosity, rock compression coefficient, the ratio of the vertical permeability to the horizontal permeability, and the injection of different media, on the development effect through the study of different development parameters of gas condensate reservoirs. The purpose of this study is to provide guidance for the rational development of condensate gas reservoirs in practical production.
Utilizing Flax Straw for Sustainable Paper Production: Delignification Methods, Structural Analysis, and Fiber Size Distribution Effects
Authors: Mashanova Nurbibi , Satayeva Zhuldyz , Smagulova Mirgul , Kundyzbayeva Nazigul , Ibzhanova Ainur , Karimova Gulmaida
Published: September 2024

This research explores the potential of agricultural waste, specifically flax straw, as a sustainable raw material for eco-friendly packaging materials. This study investigates a three-stage delignification process involving nitric acid, alkaline treatment, and organosolvent solutions. This method effectively removes lignin from the straw, resulting in high-quality technical pulp with 67.7% α-cellulose and a significantly reduced ash content (8.5%). X-ray diffraction (XRD) and thermogravimetric analysis (TGA) were employed to characterize the treated flax straw. XRD analysis revealed changes in the cellulose structure, while TGA indicated enhanced thermal stability compared to untreated straw. Microscopic analysis of the pulp fibers shows a parallel and aligned arrangement, suggesting a high fiber content and a strong paper lattice. The particle size distribution of the ground pulp, influenced by fiber size, has implications for the packing density and mechanical properties of the final product. This study demonstrates the potential of agricultural waste as a sustainable source for packaging materials, contributing to the circular economy and waste reduction.
Sorbents Based on Natural Zeolites for Carbon Dioxide Capture and Removal of Heavy Metals from Wastewater: Current Progress and Future Opportunities
Authors: Mambetova Manshuk , Dossumov Kusman , Baikhamurova Moldir , Yergaziyeva Gaukhar
Published: September 2024

This review is dedicated to the potential use of natural zeolites for wastewater treatment and carbon dioxide capture. Zeolites, due to their microporous structure and high surface activity, are used as sorbents. One effective application of zeolites is in wastewater treatment, which leads to the removal of pollutants and improvement in water quality. Zeolites can also be used for carbon dioxide capture, which helps reduce its concentration in the atmosphere and addresses climate change issues. This review examines recent research on the use of natural zeolites for the removal of heavy metals from water and CO2 capture. It explores the broad applications of natural zeolites by understanding their adsorption capabilities and the mechanisms affecting their performance in water purification from heavy metals and CO2 capture.