bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Eccentric Calcification of an Aortic Valve on the Implant Depth of a Venus-A Prosthesis During Transcatheter Aortic Valve Replacement: A Retrospective Study
Authors: Li Lanlan , Liu Yang , Jin Ping , Tang Jiayou , Lu Linhe , Zhu Guangyu , Xu Chennian , Ma Yanyan , Yang Jian
Published: August 2021

Object

Our goal was to assess the implant depth of a Venus-A prosthesis during transcatheter aortic valve replacement (TAVR) when the areas of eccentric calcification were distributed in different sections of the aortic valve.

Methods

A total of 53 patients with eccentric calcification of the aortic valve who underwent TAVR with a Venus-A prosthesis from January 2018 to November 2019 were retrospectively analyzed. The patients were divided into three groups (A, B, and C) according to the location of the eccentric calcification, which was determined by preprocedural computerized tomography angiography (CTA) images. The prosthesis release process and position were evaluated by contrast aortography during TAVR, and the differences in valve implant depths were compared among the three groups. The effects of different aortic root structures and procedural strategies on prosthesis implant depth were analyzed.

Results

Eleven patients had eccentric calcification in region A; 19 patients, in region B; and 23 patients, in region C. The patients with eccentric calcification in region B had a higher risk of prosthesis migration (10.5% upward and 21.1% downward), and the position of the prosthesis after TAVR in group B was the deepest among the three groups. When eccentric calcification was located in region A or C, the prosthesis was released at the standard position with more stability, and the location of the prosthesis was less deep after TAVR (region A: 4.12 ± 3.4 mm; region B: 10.2 ± 5.3 mm; region C: 8.4 ± 4.0 mm; region A vs. region B,P= 0.0004; region C vs. region B; andP= 0.0360). In addition, the left ventricular outflow tract (LVOT) (P= 0.0213) and aortic root angulation (P= 0.0263) also had a significant effect on implant depth in the aortic root structure of the patients. The prosthesis size was 28.3 ± 2.4 in the deep implant group and 26.4 ± 2.0 in the appropriate implant group (P= 0.0068).

Conclusion

The implant depth of the Venus-A prosthesis is closely related to the distribution of eccentric calcification in the aortic valve during TAVR. Surgeons should adjust the surgical strategy according to aortic root morphology to prevent prosthesis migration.
Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis
Authors: Hu Shu-Hsuan , Lin Shu-Fan , Huang Ya-Chen , Huang Chien-Hsun , Kuo Wen-Yu , Jinn Tsung-Luo
Published: August 2021

The manganese (Mn) tracking factor for mitochondrial Mn superoxide dismutase (MnSOD) has been annotated as yMTM1 in yeast, which belongs to the mitochondrial carrier family. We confirmed that Arabidopsis AtMTM1 and AtMTM2 are functional homologs of yMTM1 as they can revive yeast MnSOD activity in yMTM1-mutant cells. Transient expression of AtMnSOD-3xFLAG in the AtMTM1 and AtMTM2-double mutant protoplasts confirmed that AtMTM1 and AtMTM2 are required for AtMnSOD activation. Our study revealed that AtMnSOD interacts with AtMTM1 and AtMTM2 in the mitochondria. The expression levels of AtMTM1, AtMTM2, and AtMnSOD respond positively to methyl viologen (MV) and metal stress. AtMTM1 and AtMTM2 are involved in Mn and Fe homeostasis, root length, and flowering time. Transient expression of chloroplast-destined AtMnSOD revealed that an evolutionarily conserved activation mechanism, like the chloroplastic-localized MnSOD in some algae, still exists in Arabidopsis chloroplasts. This study strengthens the proposition that AtMTM1 and AtMTM2 participate in the AtMnSOD activation and ion homeostasis.
Effects of Starch Synthesis-Related Genes Polymorphism on Quality of Glutinous Rice
Authors: Zhang Ouling , Liang Cheng , Yang Bowen , You Hui , Xu Liang , Chen Yongjun , Xiang Xunchao
Published: August 2021

Glutinous rice (Oryza sativa L.) quality includes thermal properties, retrogradation and pasting viscosity properties, and so on, which have little or no amylose. However, the genetic network regulation of different quality indices has not been systematically studied. The aim was to investigate the relationship between starch synthesis-related genes (SSRGs) and the physicochemical properties of glutinous rice by targeted-gene association analysis (TGAS). The genotypes of 17 SSRGs were analyzed using 46 gene-specific molecular markers in 63 glutinous rice accessions. TGAS and gene interactions analysis indicated that soluble starch synthase (SS) IIa, SSI, starch branching enzyme (BE) IIa, and pullulanase (PUL) had significant genetic effects on glutinous rice quality. SSI and SSIIa were the major genes that regulated thermal properties and retrogradation properties (RP). PUL was central in the regulation of gel consistency (GC), and it participated in the regulation of pasting viscosity parameters (PVP) except for the pasting time and the pasting temperature. BEIIb, ISA1, SSIVb, BEIIa, SSIVa, and their interactions with SSIIa regulated gelatinization temperature (GT) and PVP. The starch properties of glutinous rice are mainly controlled by SSIIa, SSI, PUL, and their interactions, but SSIIa is central among them. These findings indicate that starch properties in glutinous rice have a complex genetic system. It provides crucial information for promoting glutinous rice quality.
Analysis of Engineered Tobacco Mosaic Virus and Potato Virus X Nanoparticles as Carriers for Biocatalysts
Authors: Schuphan Juliane , Commandeur Ulrich
Published: August 2021

Plant virus nanoparticles are promising candidates for the development of novel materials, including nanocomposites and scaffolds/carriers for functional molecules such as enzymes. Their advantages for enzyme immobilization include a modular organization, a robust and programmable structure, and a simple, cost-effective production. However, the activity of many enzymes relies on posttranslational modification and most plant viruses replicate in the cytoplasm, so functional enzymes cannot be displayed on the virus surface by direct coat protein fusions. An alternative display system to present the Trichoderma reesei endoglucanase Cel12A on potato virus X (PVX) using SpyTag/SpyCatcher (ST/SC) technology was recently developed by the authors, which allows the carrier and enzyme to be produced separately before isopeptide conjugation. Although kinetic analysis clearly indicated efficient biocatalyst activity, the PVX carrier interfered with substrate binding. To overcome this, the suitability of tobacco mosaic virus (TMV) was tested, which can also accommodate a larger number of ST peptides. We produced TMV particles displaying ST as a new platform for the immobilization of enzymes such as Cel12A, and compared its performance to the established PVX-ST platform in terms of catalytic efficiency. Although more enzyme molecules were immobilized on the TMV-ST particles, we found that the rigid scaffold and helical spacing significantly affected enzyme activity.
Identification and Characterization of a Trillin Rhamnosyltransferase From Dioscorea zingiberensis
Authors: Li Jia , Mosongo Isidore , Li Han , Wu Yalun , Li Changfu , Yang Shihui , Zhang Yansheng
Published: August 2021

Dioscorea zingiberensis accumulates abundant steroidal saponins, such as dioscin, which is the principal bioactive ingredient displaying a wide range of pharmacological activities. Diosgenin is the aglycone of dioscin, and recently, genes encoding cytochrome P450 enzymes in the late steps of diosgenin biosynthesis have been isolated. Diosgenin was successfully synthesized in the cholesterol-producing yeasts. From diosgenin to dioscin, one glucose and two rhamnose groups need to be added. Although genes encoding UDP-glucosyltransferases converting diosgenin to trillin were isolated, genes encoding UDP-rhamnosyltransferases involved in dioscin biosynthesis remain unknown. In this study, we isolated the cDNA encoding the trillin rhamnosyltransferase (designated DzGT1) fromD. zingiberensis. Heterologous expression of DzGT1 inEscherichia colicells showed that the gene product exhibits an enzyme activity that glycosylates the trillin to form prosapogenin A of dioscin (PSA). The transcript level ofDzGT1is in accord with PSA accumulation in different organs ofD. zingiberensis. Integration of the biochemical, metabolic, and transcriptional data supported the function of DzGT1 in dioscin biosynthesis. The identification and characterization of DzGT1 will help understand the metabolism of steroidal saponins inD. zingiberensisand provide candidate UDP-rhamnosyltransferase for efficient production of PSA, dioscin, and relevant steroidal saponins in microbial hosts.
Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice
Authors: Liu Xue-jiao , Sun Jiaqi , Huang Yuqing , Li Chao , Zheng Peng , Yuan Yue , Chen Hao , Jan Mehmood , Zheng Huanquan , Du Hao , Tu Jumin
Published: August 2021

Chloroplasts are crucial organelles for the generation of fatty acids and starch required for plant development. Nascent polypeptide-associated complex (NAC) proteins have been implicated in development as transcription factors. However, their chaperone roles in chloroplasts and their relationship with pollen development in plants remain to be elucidated. Here, we demonstrated that Osj10gBTF3, a NAC protein, regulates pollen and chloroplast development in rice by coordinating with a Hsp90 family chaperone OsHSP82 to mediate chloroplast import. Knockout of Osj10gBTF3 affects pollen and chloroplast development and significantly reduces the accumulation of fertility-related chloroplast protein OsPPR676. Both Osj10gBTF3 and OsHSP82 interact with OsPPR676. Interestingly, the interaction between OsHSP82 and OsPPR676 is only found in the cytoplasm, while the interaction between Osj10gBTF3 and OsPPR676 also occurs inside the chloroplast. The chloroplast stroma chaperone OsCpn60 can also be co-precipitated with Osj10gBTF3, but not with OsHSP82. Further investigation indicates that Osj10gBTF3 enters the chloroplast stroma possibly through the inner chloroplast membrane channel protein Tic110 and then recruits OsCpn60 for the folding or assembly of OsPPR676. Our results reveal a chaperone role of Osj10gBTF3 in chloroplast import different from Hsp90 and provide a link between chloroplast transport and pollen development in rice.
Feasibility of a Responsibility-Based Leadership Training Program for Novice Physical Activity Instructors
Authors: Toivonen Hanna-Mari , Hassandra Mary , Wright Paul M. , Hagger Martin S. , Hankonen Nelli , Laine Kaarlo , Lintunen Taru
Published: August 2021

Most coaches and instructors would like to teach more than just sport skills to their athletes and children. However, to promote athletes’ or children’s holistic development and teach them to take responsibility and lead, requires the coaches and instructors to first master the skills themselves. Therefore, feasible, high quality leadership training programs where coaches and physical activity instructors are taught to teach and share leadership are needed. The aim of the current study was to evaluate the feasibility of a leadership training program to optimize it and to determine whether to proceed with its evaluation. In the leadership training program, eight Finnish novice physical activity instructors, aged 18 to 22, were taught to promote positive youth development, personal and social responsibility, and shared leadership in a physical activity context. The participants had minimal to no leadership training or experience. The training program consisted of seven meetings totaling 20 h. Helllison’s teaching personal and social responsibility (TPSR) model was the theoretical and practical framework of the training program. Feasibility of the leadership training program was evaluated across four domains of an evidence-based framework: demand, practicality, acceptability, and implementation fidelity. Data of the current complex intervention were collected with application videos, questionnaires, researcher’s log, lesson plans, video recordings, and a semi-structured focus group interview. The quantitative data were analyzed using descriptive statistics and the qualitative data using deductive and inductive content analysis. There was a demand for the leadership training program. The training program was perceived as practical and highly acceptable by the novice instructors and the trainers, and implemented with fidelity, indicating high overall feasibility. No implementation issues were found. Consequently, the current leadership training program has a high probability of efficacy and can be accepted for further evaluation.
The Impact of Parental Relationship Satisfaction on Infant Development: Results From the Population-Based Cohort Study DREAM
Authors: Nicolaus Caroline , Kress Victoria , Kopp Marie , Garthus-Niegel Susan
Published: August 2021

Extensive literature has shown that interparental conflicts and violence have detrimental effects on children's adjustment in childhood and adolescence. It is not equally well-understood how parental relationship satisfaction impacts infant communicational and personal-social development during the first year of life. This longitudinal study examines (a) the impact of maternal and paternal relationship satisfaction on infant development, (b) whether this prospective association is mediated by parent-infant relationship, and (c) a potential moderating effect of infant gender. Data were derived from the population-based cohort study “Dresden Study on Parenting, Work, and Mental Health” (DREAM) including 1,012 mothers and 676 fathers. Relationship satisfaction and parent-infant relationship were assessed eight weeks postpartum, infant communicational and personal-social development were measured 14 months postpartum. Multiple linear regression, mediation, and moderation analyses were conducted for mothers and fathers separately. It was shown that paternal relationship satisfaction is a significant predictor of infant personal-social development. This prospective association was partially mediated by father-infant relationship. When postnatal depression was included in the analysis, however, father-infant relationship was not a significant mediator. The association in fathers is neither reduced nor increased as a function of infant gender. No similar effects were found in the mothers' sample. Parental relationship satisfaction did not significantly predict infant communicational development in either mothers or fathers. The study findings highlight the importance of paternal relationship satisfaction, father-infant relationship, and postnatal depression for infant personal-social development.
Pre-verbal Children’s Participation in a New Key. How Intersubjectivity Can Contribute to Understanding and Implementation of Child Rights in Early Childhood
Authors: Våpenstad Eystein Victor , Bakkenget Brynulf
Published: August 2021

Children’s participation and involvement has increasingly been on the agenda for the last few decades. The right for children to participate was established in the United Nations Convention on the Rights of the Child (UNCRC). However, even though the UNCRC gives the right to participate to all children, national policy and practice seems to draw a line on verbal language and exclude pre-verbal infants from participation. The spur of this paper is to challenge the exclusion of infants, to describe how pre-linguistic children communicate their intentions, and to show how an understanding of children’s participation grounded in intersubjectivity, can inform and reframe the participation of all children as being fundamentally about close relationships with sensitive and containing adults who look within themselves for the voice of the child. The infant’s proto-conversational narrative communicates interests and feelings through sympathetic rhythms of what infant researchers have named “communicative musicality,” and it can surface in the mother’s narrative about the child and their relationship. Intersubjectivity oppose the monadic view of man as separate and left only to imitate others and claims that humans from the very start are intertwined in a fundamental thirdness of co-created reality. Infants are powerful communicators who actively engage in intersubjective relationships with their caretakers only days after birth, and newborns actively influence and even control the mental process of those who communicate with them. Early childhood participation then, would be to find within ourselves the voice of the child. A research project building on the theories and ideas described in the first part of the article, is presented.
A Multilevel Person-Centered Examination of Teachers' Workplace Experiences: Replication and Extension With Links to Instructional Support and Achievement
Authors: Collie Rebecca J. , Martin Andrew J. , Morin Alexandre J. S. , Malmberg Lars-Erik , Sammons Pamela
Published: August 2021

In a replication and extension of an earlier study, we relied on person-centered analyses to identify teacher (Level 1) and school (Level 2) profiles based on teachers' experiences of job demands (barriers to professional development, disruptive student behavior), job resources (teacher collaboration, input in decision-making), and personal resources (self-efficacy). We examined data from 5,439 teachers working in 364 schools in Australia and 2,216 teachers working in 149 schools in England. Latent profile analysis revealed six teacher profiles: Low-Demand-Flourisher (11%), Mixed-Demand-Flourisher (17%), Job-Resourced-Average (11%), Balanced-Average (14%), Mixed-Resourced-Struggler (11%), and Low-Resourced-Struggler (36%). Two school profiles were identified: an Unsupportive school profile (43%) and a Supportive school profile (57%). Several significant relations between these profiles and teacher/school characteristics and work-related outcomes were also identified at both levels. Although our results generally replicated prior findings, some differences were also observed, possibly as a results of recent changes in policies regarding in teacher support and accountability. Next, we extended prior work using a subsample of the Australian teachers for whom we had matching student data. This second set of results revealed that schools with a greater proportion of low-SES students were more likely to present an Unsupportive school profile. Moreover, the Supportive school profile was associated with higher levels of student-reported instructional support and school-average achievement in reading, mathematics, and science.
Screen Time and Autism: Current Situation and Risk Factors for Screen Time Among Pre-school Children With ASD
Authors: Dong Han-Yu , Feng Jun-Yan , Wang Bing , Shan Ling , Jia Fei-Yong
Published: August 2021

Objective: To investigate the current status of screen time in children with ASD, its correlation with autistic symptoms and developmental quotient (DQ), and the factors affecting screen time.

Method: One hundred ninety-three Chinese children with ASD were recruited. We collected the demographic and screen time data using a questionnaire. The ASD core symptoms and developmental quotient (DQ) were measured by the Autism Behavior Checklist (ABC), Childhood Autism Rating Scale (CARS), Autism Diagnostic Observation Schedule-Second Edition (ADOS-2), Griffiths Development Scales-Chinese Language Edition (GDS-C), and Chinese Children's Parent-Child Relationship Questionnaire (CPCIS). Then, we analyzed the correlations between the screen time of children with ASD and the ABC, CARS, ADOS, GDS-C DQs, and CPCIS scores. Linear regression was used to analyze the risk factors that affect screen time.

Results: The children's average daily screen time was 2.64 ± 2.24 h. Forty eight percent children were exposed to two or more types of electronic devices. Their favorite activity of screen time was watching cartoons. Only 34% children spent screen time accompanied by parents and with communication. 50.26% children had no screen time before sleeping. The screen time of children with ASD had a negative correlation with the GDS-C CQ (r = −0.234, P = 0.001) and the CPCIS score (r = −0.180, P = 0.012) and a positive correlation with the CARS score (r = 0.192, P = 0.009). A low father's education level (P = 0.010), less restriction of the child's screen time by the guardian (P = 0.001), greater caregiver screen time (P < 0.001), the use of the screen as a tool for child rearing (P = 0.001), and the child's ownership of independent electronic equipment (P = 0.027) are risk factors for long screen time in children with ASD.

Conclusion: The screen time of children with ASD in China is higher than the recommended standard, and the current situation is serious. The screen time of ASD children is related to their autism symptoms, DQ and parent-child interaction. Low paternal education levels, less restriction of children's screen time by guardians, greater guardian screen time, the use of screens in child rearing, and children's ownership of independent electronic equipment can lead to an increase in children's screen time. These findings may have implications for family intervention strategies.
The Effectiveness of Eye Movement Desensitization and Reprocessing Toward Adults With Major Depressive Disorder: A Meta-Analysis of Randomized Controlled Trials
Authors: Yan Shuya , Shan Yanyan , Zhong Shuming , Miao Haofei , Luo Yange , Ran Hanglin , Jia Yanbin
Published: August 2021

The practice-based evidence suggests that it is possible to use eye movement desensitization and reprocessing (EMDR) to treat major depressive disorder (MDD), but its specific efficacy is unknown. A systematic search was carried out for randomized controlled trials comparing EMDR with a control condition group in MDD patients. Two meta-analyses were conducted, with symptom reduction as primary outcome and remission as exploratory outcome. Eight studies with 320 participants were included in this meta-analysis. The first meta-analysis showed that EMDR outperformed “No Intervention” in decreasing depressive symptoms (standardized mean difference [SMD] = −0.81, 95% CI = −1.22 to −0.39, p < 0.001, low certainty), but statistically significant differences were not observed in improving remission (risk ratio = 1.20, 95% CI = 0.87–1.66, p = 0.25, very low certainty). The second showed the superiority of EMDR over CBT in reducing depressive symptoms (mean difference [MD] = −7.33, 95% CI = −8.26 to −6.39, p < 0.001, low certainty), and improving remission (risk ratio = 1.95, 95% CI = 1.24–3.06, p = 0.004, very low certainty). Besides, anxiety symptoms and level of functioning could not be included as secondary outcome due to the lack of data. The present meta-analysis suggests that EMDR is more effective in treating MDD than “No Intervention” and CBT, particularly in individuals who have traumatic experience. However, this result should be considered with caution due to small sample size and low quality of trails.
Do Migration Characteristics Influence the Utilization of Basic Public Health Services in Internal Elderly Migrants in China?
Authors: Lin Yanwei , Wang Tingxian , Zhu Tingting
Published: August 2021

Background: The literature shows that migration characteristics are a potential pathway through which migration can influence basic healthcare service utilization. The goal of the study was to explore the effect of migration characteristics on the utilization of basic public health services for internal elderly migrants in China and to identify the pathways that might promote their utilization of basic public health services.

Methods: We studied 1,544 internal elderly migrants. The utilization of basic public health services was determined through participation in free health checkups organized by community health service institutions in the past year. Migration characteristics were represented by years of residence and reasons for migration. Other variables included demographic characteristics and social factors, e.g., the number of local friends and exercise time per day were measured to represent social contacts. Multivariate binary logistic regression was employed to explore the association of the variables with the likelihood of using community health services.

Results: A total of 55.6% of respondents were men, and the mean age was 66.34 years (SD 5.94). A low level of education was observed. A total of 59.9% of migrants had been residents for over 10 years, and the main reason for migrating was related to family. Of these migrants, 12.9% had no local friends. Furthermore, 5.2% did not exercise every day. Social contacts were complete mediators of the impact of migration characteristics on the utilization of primary healthcare.

Conclusion: Our study highlighted the mediating role of social factors in the relationship between migration characteristics and the utilization of basic public health services among Chinese internal elderly migrants. The findings supported the need to increase the opportunities for social contacts between local elderly individuals and internal elderly migrants.
Colorless Windows That Transform Sunlight Into Electricity
Authors: Vohra Varun , Sano Takashi
Published: August 2021

Solar cells help us use less coal or oil when we make electricity. This way, we cause fewer problems for the environment. Most solar panels are expensive, and they cannot be used for windows because they are not transparent, so they hide your view. But solar windows can be created with special chemicals called organic colorants. These windows can catch sunlight and use its energy to make electricity, but you can still see a beautiful view through them. The windows we made are inexpensive and keep the colors of your view unchanged. Each colorless window makes 100 watts of electricity, which is enough to recharge 20 smartphones at the same time. Because we can use these cheap, colorless, solar windows everywhere, we could make a lot of clean energy!
Resilient Governance Regimes That Support Urban Agriculture in Sub-Saharan Cities: Learning From Local Challenges
Authors: Vidal Merino Mariana , Gajjar Sumetee Pahwa , Subedi Aasha , Polgar Ana , Van Den Hoof Catherine
Published: August 2021

Local governments in Sub-Saharan Africa face the daunting task of high urban growth and potentially devastating impacts of climate change across local communities and the economy. Urban and peri-urban food production can be among nature-based strategies planned for improving urban food security, reducing emissions, and climate adaptation. Co-operative governance, strategic planning, and accountable institutions are needed to support urban agriculture (UA), in the face of climate risks, unplanned urban development, the gendered nature of food provision, and the inability of urban farmers to self-organize toward optimal market and land access outcomes. Using a case study approach guided by qualitative content analysis with information derived from web analysis, we apply the Institutional Analysis and Development Framework to analyze underlying governance factors for UA in three selected Sub-Saharan African cities. Our three case cities of Kampala, Tamale and Cape Town reveal that UA is beginning to receive policy attention toward food security, and recognition for generating environmental, ecological, health, and human well-being benefits. Literature from specific cities however does not yet signal a local awareness and policy thrust regarding the associated and pertinent climate adaptation benefits of urban agriculture. We therefore recommend trans-disciplinary, locally-led, planning-based, and multi-sectoral approaches, involving a range of stakeholders toward recognizing and achieving the climate adaptation, environmental (ecologically restorative) and food security benefits of pursuing urban agriculture. This signals a larger role for the practice in sustainability discourse and SDGs 2 and 11, scaling out and up across large, medium and small towns, and cities of Sub-Saharan Africa.
Drivers of Millet Consumption Among School Aged Children in Central Tanzania
Authors: Chande Monica , Muhimbula Happiness , Mremi Ruth , Muzanila Yasinta C. , Kumwenda Nelson C. , Msuya John , Msere Harry , Bekunda Mateete , Okori Patrick , Gichohi-Wainaina Wanjiku N.
Published: August 2021

Background: Iron and zinc deficiency are common public health problems in low-income countries largely due to poor consumption of iron and zinc rich foods. It has previously been observed that 57% of school aged children (SAC) in Tanzania suffer from anemia. In addition, estimates indicate that over 25% of the population have inadequate zinc intake. Pearl millet is an example of a nutrient dense, resilient cereal crop, that can be promoted to diversify diets and combat iron and zinc deficiency. This study overall aim was to increase pearl millet consumption among school aged (5 – 12 years) children. As part of the study, we investigated, the drivers of food choice relating to pearl millet consumption.

Methods: The study was a cross-sectional study of randomly selected households in Kongwa district, Dodoma region of Tanzania. In total, 128 women of reproductive age (20 – 49 years) were randomly selected for the study. A study questionnaire consisting of 66 items, was developed and validated. The constructs in the questionnaire were categorized in two groups: internal and external factors. Respondents were asked to indicate their level of agreement or disagreement with statements read to them by interviewers. The scores on intention and behavior constructs were based on the number of times caregivers intended to, or had fed their school going children with pearl millet in the referent month. Intention was considered high if it was higher than the median intention score of the group, and low if it was equal to or lower than the median scores. Correlations and multiple linear regressions were performed to measure association between constructs and to identify predictive constructs. The Mann-Whitney U test was used for score comparison.

Results: There was a significant difference between intention and behavior among those who did not consume pearl millet (P = 0.003), and those who consumed pearl millet two or more times a week, in the same month (P = 0.01). Knowledge was significantly correlated with behavior identity (ρ = 0.58, P = 0.001), while health behavior identity was significantly correlated with intention (ρ = 0.31, P = 0.001). Intention of caregivers was significantly and positively correlated (ρ = 0.44, P = 0.001) with and predicted consumption of pearl millet (ρ = 0.87, P = 0.067).

Conclusion: Increasing knowledge or awareness on nutritional benefits of pearl millet among caregivers may increase consumption of pearl millet by children of school going age.
Recanalization in Uncut Roux-en-Y Reconstruction: An Animal Experiment and a Clinical Study
Authors: Wu Feng , Ni Zhizhan , Diao Hongliang , Huang Chenshen , Wang Song , Ge Bujun , Huang Qi
Published: August 2021

Background: Because of the challenge of jejunal closure recanalization, uncut Roux-en-Y reconstruction remains controversial. This study aimed to investigate the incidence of recanalization after uncut Roux-en-Y reconstruction in pigs and a small number of patients.

Methods: Twenty miniature pigs were subjected to distal gastrectomy and uncut Roux-en-Y reconstruction using various rows of linear staplers to block the intestine. The pigs were sacrificed, and the incidence of recanalization was investigated 1 month after the operation. From December 2018 to June 2019, 10 patients with gastric cancer who had undergone elective laparoscopy-assisted distal gastrectomy and uncut Roux-en-Y reconstruction were included in this study. The primary study outcome was recanalization of the afferent limb, demonstrated by gastrointestinal radiography 1, 3, and 6 months after surgery. Various numbers of staple lines across the afferent jejunal limb were applied for closure: 2 staple lines in 2 pigs, 4 staple lines in 6 pigs, 6 staple lines in 8 pigs, and 8 staple lines in 4 pigs.

Results: Complete recanalization was detected in all 20 pigs 1 month postoperatively. Recanalization was detected in five cases (50%) by gastrointestinal radiography. Among them, 1 case of recanalization was found in the 1st month after the operation, 2 cases were found in the 3rd month, and another 2 cases were found in the 6th month. Bile reflux was detected by endoscopy in 2 patients with recanalization.

Conclusions: The occurrence of afferent limb recanalization after uncut Roux-en-Y reconstruction is high, and using additional staplers alone cannot decrease the incidence of recanalization. Based on our study, uncut Roux-en-Y reconstruction is not recommended.
The Emergence and Future of Public Health Data Science
Authors: Goldsmith Jeff , Sun Yifei , Fried Linda P. , Wing Jeannette , Miller Gary W. , Berhane Kiros
Published: August 2021

Data science is a newly‐formed and, as yet, loosely‐defined discipline that has nonetheless emerged as a critical component of successful scientific research. We seek to provide an understanding of the term “data science,” particularly as it relates to public health; to identify ways that data science methods can strengthen public health research; to propose ways to strengthen education for public health data science; and to discuss issues in data science that may benefit from a public health perspective.
Trajectory Tracking Control for Reaction–Diffusion System with Time Delay Using P-Type Iterative Learning Method
Authors: Liu Yaqiang , Li Jianzhong , Jin Zengwang
Published: August 2021

This paper has dealt with a tracking control problem for a class of unstable reaction–diffusion system with time delay. Iterative learning algorithms are introduced to make the infinite-dimensional repetitive motion system track the desired trajectory. A new Lyapunov–Krasovskii functional is constructed to deal with the time-delay system. Picewise distribution functions are applied in this paper to perform piecewise control operations. By using Poincaré–Wirtinger inequality, Cauchy–Schwartz inequality for integrals and Young’s inequality, the convergence of the system with time delay using iterative learning schemes is proved. Numerical simulation results have verified the effectiveness of the proposed method.
Pathophysiology of Fever and Application of Infrared Thermography (IRT) in the Detection of Sick Domestic Animals: Recent Advances
Authors: Mota-Rojas Daniel , Wang Dehua , Titto Cristiane Gonçalves , Gómez-Prado Jocelyn , Carvajal-de la Fuente Verónica , Ghezzi Marcelo , Boscato-Funes Luciano , Barrios-García Hugo , Torres-Bernal Fabiola , Casas-Alvarado Alejandro , Martínez-Burnes Julio
Published: August 2021

Body-temperature elevations are multifactorial in origin and classified as hyperthermia as a rise in temperature due to alterations in the thermoregulation mechanism; the body loses the ability to control or regulate body temperature. In contrast, fever is a controlled state, since the body adjusts its stable temperature range to increase body temperature without losing the thermoregulation capacity. Fever refers to an acute phase response that confers a survival benefit on the body, raising core body temperature during infection or systemic inflammation processes to reduce the survival and proliferation of infectious pathogens by altering temperature, restriction of essential nutrients, and the activation of an immune reaction. However, once the infection resolves, the febrile response must be tightly regulated to avoid excessive tissue damage. During fever, neurological, endocrine, immunological, and metabolic changes occur that cause an increase in the stable temperature range, which allows the core body temperature to be considerably increased to stop the invasion of the offending agent and restrict the damage to the organism. There are different metabolic mechanisms of thermoregulation in the febrile response at the central and peripheral levels and cellular events. In response to cold or heat, the brain triggers thermoregulatory responses to coping with changes in body temperature, including autonomic effectors, such as thermogenesis, vasodilation, sweating, and behavioral mechanisms, that trigger flexible, goal-oriented actions, such as seeking heat or cold, nest building, and postural extension. Infrared thermography (IRT) has proven to be a reliable method for the early detection of pathologies affecting animal health and welfare that represent economic losses for farmers. However, the standardization of protocols for IRT use is still needed. Together with the complete understanding of the physiological and behavioral responses involved in the febrile process, it is possible to have timely solutions to serious problem situations. For this reason, the present review aims to analyze the new findings in pathophysiological mechanisms of the febrile process, the heat-loss mechanisms in an animal with fever, thermoregulation, the adverse effects of fever, and recent scientific findings related to different pathologies in farm animals through the use of IRT.
Virgin Polystyrene Microparticles Exposure Leads to Changes in Gills DNA and Physical Condition in the Mediterranean Mussel Mytilus Galloprovincialis
Authors: Masiá Paula , Ardura Alba , García-Vázquez Eva
Published: August 2021

The ever-growing concentration of microplastics in the marine environment is leading to a plethora of questions regarding marine organisms’ present and future health status. In this article, the Mediterranean mussel (Mytilus galloprovincialis), a commercial species distributed worldwide, has been exposed to 21 daily doses of polystyrene microparticles (10 µm) at four different concentrations that are environmentally realistic (control: no microplastics, C1: 0.02 mg/L, C2: 0.2 mg/L, and C3: 2 mg/L). The physical status through the condition index, and damages in DNA integrity in gills, through DNA fragmentation, were determined. Results showed a minor effect on DNA integrity but a worse physical status at higher doses. Results could be interpreted as a decrease in mussel feeding activity/filtration rates when exposed to high microplastic concentrations, thus reducing the direct exposure to microplastics in gills. These effects could be happening currently and/or may happen in the near future, threatening populations inhabiting microplastics-polluted environments.
In Vitro Antibacterial Effect of the Methanolic Extract of the Korean Soybean Fermented Product Doenjang against Staphylococcus aureus
Authors: Lalouckova Klara , Mala Lucie , Marsik Petr , Skrivanova Eva
Published: August 2021

Ultra-high performance liquid chromatography/mass spectrometry showed soyasaponin I and the isoflavones daidzein, genistein, and glycitein to be the main components of the methanolic extract of the Korean soybean fermented product doenjang, which is known to be a rich source of naturally occurring bioactive substances, at average contents of 515.40, 236.30, 131.23, and 29.00 ng/mg, respectively. The antimicrobial activity of the methanolic extract of doenjang against nine Staphylococcusaureus strains was determined in vitro by the broth microdilution method to investigate its potential to serve as an alternative antibacterial compound. The results suggest that the extract is an effective antistaphylococcal agent at concentrations of 2048–4096 µg/mL. Moreover, the tested extract also showed the ability to inhibit the growth of both methicillin-sensitive and methicillin-resistant animal and clinical S. aureus isolates. The growth kinetics of the chosen strains of S. aureus at the minimum inhibitory concentration of the methanolic extract of doenjang support the idea that the tested extract acts as an antibacterial compound. To the best of our knowledge, this is the first report on the antistaphylococcal action of the methanolic extract of doenjang thus, additional studies including in vivo testing are necessary to confirm this hypothesis.
Diabetes and Alzheimer’s Disease: Might Mitochondrial Dysfunction Help Deciphering the Common Path?
Authors: Potenza Maria Assunta , Sgarra Luca , Desantis Vanessa , Nacci Carmela , Montagnani Monica
Published: August 2021

A growing number of clinical and epidemiological studies support the hypothesis of a tight correlation between type 2 diabetes mellitus (T2DM) and the development risk of Alzheimer’s disease (AD). Indeed, the proposed definition of Alzheimer’s disease as type 3 diabetes (T3D) underlines the key role played by deranged insulin signaling to accumulation of aggregated amyloid beta (Aβ) peptides in the senile plaques of the brain. Metabolic disturbances such as hyperglycemia, peripheral hyperinsulinemia, dysregulated lipid metabolism, and chronic inflammation associated with T2DM are responsible for an inefficient transport of insulin to the brain, producing a neuronal insulin resistance that triggers an enhanced production and deposition of Aβ and concomitantly contributes to impairment in the micro-tubule-associated protein Tau, leading to neural degeneration and cognitive decline. Furthermore, the reduced antioxidant capacity observed in T2DM patients, together with the impairment of cerebral glucose metabolism and the decreased performance of mitochondrial activity, suggests the existence of a relationship between oxidative damage, mitochondrial impairment, and cognitive dysfunction that could further reinforce the common pathophysiology of T2DM and AD. In this review, we discuss the molecular mechanisms by which insulin-signaling dysregulation in T2DM can contribute to the pathogenesis and progression of AD, deepening the analysis of complex mechanisms involved in reactive oxygen species (ROS) production under oxidative stress and their possible influence in AD and T2DM. In addition, the role of current therapies as tools for prevention or treatment of damage induced by oxidative stress in T2DM and AD will be debated.
Core Circadian Clock Proteins as Biomarkers of Progression in Colorectal Cancer
Authors: Aroca-Siendones María I. , Moreno-SanJuan Sara , Puentes-Pardo Jose D. , Verbeni Michela , Arnedo Javier , Escudero-Feliu Julia , García-Costela María , García-Robles Adelina , Carazo Ángel , León Josefa
Published: August 2021

Colorectal cancer (CRC) is one of the most common tumours in developed countries. Although its incidence and mortality rates have decreased, its prognosis has not changed, and a high percentage of patients with CRC develop relapse (metachronous metastasis, MM, or local recurrence, LR) during their disease. The identification of these patients is very important for their correct management, but the lack of prognostic markers makes it difficult. Given the connection between circadian disruption and cancer development and progression, we aimed to analyse the prognostic significance of core circadian proteins in CRC. We measured the expression of PER1-3, CRY1-2, BMAL1 and NR1D2 in a cohort of CRC patients by immunohistochemistry (IHC) and analysed their prognostic potential in this disease. A low expression of PER2 and BMAL1 was significantly associated with metastasis at the moment of disease diagnosis, whereas a high expression of CRY1 appeared as an independent prognostic factor of MM development. A high expression of NR1D2 appeared as an independent prognostic factor of LR development after disease diagnosis. Moreover, patients with a low expression of BMAL1 and a high expression of CRY1 showed lower OS and DFS at five years. Although these markers need to be validated in larger and different ethnic cohorts, the simplicity of IHC makes these proteins candidates for personalizing CRC treatment.
Modulated Neuroprotection in Unresponsive Wakefulness Syndrome after Severe Traumatic Brain Injury
Authors: Daia Cristina , Scheau Cristian , Spinu Aura , Andone Ioana , Popescu Cristina , Toader Corneliu , Bumbea Ana Maria , Verenca Madalina Codruta , Onose Gelu
Published: August 2021

Background: We aimed to assess the effects of modulated neuroprotection with intermittent administration in patients with unresponsive wakefulness syndrome (UWS) after severe traumatic brain injury (TBI). Methods: Retrospective analysis of 60 patients divided into two groups, with and without neuroprotective treatment with Actovegin, Cerebrolysin, pyritinol, L-phosphothreonine, L-glutamine, hydroxocobalamin, alpha-lipoic acid, carotene, DL-α-tocopherol, ascorbic acid, thiamine, pyridoxine, cyanocobalamin, Q 10 coenzyme, and L-carnitine alongside standard treatment. Main outcome measures: Glasgow Coma Scale (GCS) after TBI, Extended Glasgow Coma Scale (GOS E), Disability Rankin Scale (DRS), Functional Independence Measurement (FIM), and Montreal Cognitive Assessment (MOCA), all assessed at 1, 3, 6, 12, and 24 months after TBI. Results: Patients receiving neuroprotective treatment recovered more rapidly from UWS than controls (p = 0.007) passing through a state of minimal consciousness and gradually progressing until the final evaluation (p = 0.000), towards a high cognitive level MOCA = 22 ± 6 points, upper moderate disability GOS-E = 6 ± 1, DRS = 6 ± 4, and an assisted gait, FIM =101 ± 25. The improvement in cognitive and physical functioning was strongly correlated with lower UWS duration (−0.8532) and higher GCS score (0.9803). Conclusion: Modulated long-term neuroprotection may be the therapeutic key for patients to overcome UWS after severe TBI.
Regular Vigorous-Intensity Physical Activity and Walking Are Associated with Divergent but not Convergent Thinking in Japanese Young Adults
Authors: Chen Chong , Mochizuki Yasuhiro , Hagiwara Kosuke , Hirotsu Masako , Nakagawa Shin
Published: August 2021

The beneficial effects of regular physical activity (PA) on cognitive functions have received much attention. Recent research suggests that regular PA may also enhance creative thinking, an indispensable cognitive factor for invention and innovation. However, at what intensity regular PA brings the most benefits to creative thinking remains uninvestigated. Furthermore, whether the levels of regular PA affect the acute PA effects on creative thinking is also unclear. In the present study, using a previous dataset that investigated the effects of an acute bout of aerobic exercise on creative thinking in healthy Japanese young adults (22.98 ± 1.95 years old) in the year 2020, we tested the association between different intensities of regular PA (i.e., vigorous, moderate, and walking) and creative thinking with the cross-sectional baseline data using multiple linear regression. We also investigated whether regular PA levels were associated with the acute aerobic exercise intervention effects on creative thinking. The results showed that cross-sectionally, the regular PAs were differentially associated with divergent but not convergent thinking. Specifically, whereas the amount of vigorous-intensity PA was positively associated with fluency and flexibility, the amount of walking was positively associated with novelty on the alternate uses test (AUT) measuring divergent thinking. Importantly, the explained variances of fluency, flexibility, and novelty were 20.3% (p = 0.040), 18.8% (p = 0.055), and 20.1% (p = 0.043), respectively. None of the regular PAs predicted convergent thinking (i.e., an insight problem-solving task), nor were they associated with the acute aerobic exercise intervention effects on divergent and convergent thinking. These findings suggest that engaging in regular vigorous-intensity PA and walking may be useful strategies to enhance different aspects of divergent thinking in daily life.