bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Clinical Nomogram for Predicting Overall Survival in Patients with Emergency Surgery for Colorectal Cancer
Authors: Constantin Georgiana Bianca , Firescu Dorel , Mihailov Raul , Constantin Iulian , Ștefanopol Ioana Anca , Iordan Daniel Andrei , Ștefănescu Bogdan Ioan , Bîrlă Rodica , Panaitescu Eugenia
Published: March 2023

Background: Long-term survival after emergency colorectal cancer surgery is low, and its estimation is most frequently neglected, with priority given to the immediate prognosis. This study aimed to propose an effective nomogram to predict overall survival in these patients. Materials and methods: We retrospectively studied 437 patients who underwent emergency surgery for colorectal cancer between 2008 and 2019, in whom we analyzed the clinical, paraclinical, and surgical parameters. Results: Only 30 patients (6.86%) survived until the end of the study. We identified the risk factors through the univariate Cox regression analysis and a multivariate Cox regression model. The model included the following eight independent prognostic factors: age > 63 years, Charlson score > 4, revised cardiac risk index (RCRI), LMR (lymphocytes/neutrophils ratio), tumor site, macroscopic tumoral invasion, surgery type, and lymph node dissection (p < 0.05 for all), with an AUC (area under the curve) of 0.831, with an ideal agreement between the predicted and observed probabilities. On this basis, we constructed a nomogram for prediction of overall survival. Conclusions: The nomogram created, on the basis of a multivariate logistic regression model, has a good individual prediction of overall survival for patients with emergency surgery for colon cancer and may support clinicians when informing patients about prognosis.
Dynamic Aspects of Pre-Soft Contact Lens Tear Film and Their Relation to Dry Eye: Basic Science and Clinical Relevance
Authors: Yokoi Norihiko , Eftimov Petar , Georgiev Georgi As.
Published: March 2023

Soft contact lens (SCL) perturbs the intimate connection between the pre-lens tear film (PLTF) and the ocular surface in various ways, i.e., (i) decrease in tear meniscus radius and aqueous tear thickness, (ii) attenuation of tear film lipid layer spread, (iii) limited wettability of SCL surface, (iv) increased friction with eyelid wiper, etc. This often results in SCL-related dry eye (SCLRDE) manifested as PLTF instability and contact lens discomfort (CLD). In this review, the individual contributions of factors (i–iv) to PLTF breakup patterns (BUP) and CLD are considered via the tear film-oriented diagnosis framework adopted by the Asia Dry Eye Society from a clinical and basic science perspective. It is shown that SCLRDE (due to aqueous deficiency, increased evaporation, or decreased wettability) and BUP of PLTF classify within the same types as the ones observed for the precorneal tear film. The analysis of PLTF dynamics reveals that the inclusion of SCL enhances the manifestation of BUP associated with (i) decreased thickness of PLTF aqueous layer and (ii) limited SCL wettability as shown by the rapid expansion of BUP area. PLTF thinness and instability result in increased blink-related friction and lid wiper epitheliopathy as major contributor to CLD.
Relationship between the Prevalence of Metabolic Disease and Impaired Mobility, Diet, Physical Activity, and Socio-Demographic Characteristics in the Polish Elderly—A Cross-Sectional Study
Authors: Jeżewska-Zychowicz Marzena , Gajda Robert
Published: March 2023

Maintaining good health for as long as possible presents a great challenge for the elderly. As the elderly population is growing, there is an ongoing need to identify health risk factors affecting older individuals. The study aimed to explore relationships between sociodemographic characteristics, diet, physical activity, and prevalence of metabolic diseases and impaired mobility in the Polish elderly. A cross-sectional study was carried out on 417 elderlies in May–July 2021. Cluster analysis was applied to separate four homogeneous clusters based on the prevalence of metabolic disease and impaired mobility. Logistic regression analysis was used to verify associations between variables. Being overweight or having obesity, as well as following a diet, increased the probability of being affected by metabolic disease. Being well educated, having a better financial situation, positive perception of own health, and having at least moderate physical activity decreased the probability of suffering from mobility impairments. Eating behaviors were not found to be predictors of the disease. However, they differentiated the selected clusters. The results confirmed the heterogeneity of factors that may impact healthy aging. Thus, they should be taken into account by public health authorities to develop health promotion actions adjusted to the needs of specific subgroups.
The Effects of Freezing Media on the Characteristics of Male and Female Chicken Primordial Germ Cell Lines
Authors: Ecker András , Lázár Bence , Tóth Roland Imre , Urbán Martin , Tokodyné Szabadi Nikolett , Salinas Aponte Maria Teresa , Adnan Mohd , Várkonyi Eszter , Gócza Elen
Published: March 2023

Recently, in vitro gene preservation has gained ground thanks to its lower cost and higher stability compared to in vivo techniques. One of the methods that can preserve female-specific W chromosome-linked genes is primordial germ cell (PGC) freezing. PGCs can be isolated from Hamburger–Hamilton stage 14–16 embryos via blood sampling. In our experiment, we used two newly established Black Transylvanian naked neck chicken cell lines and four cell lines from our gene bank. We compared two different freezing media (FAM1 and FAM2) in this study. The cell number and viability of the PGCs were measured before freezing (BF) and after thawing on Day 0, Day 1, and Day 7 of cultivation. We analyzed the germ cell-specific chicken vasa homologue (CVH) expression profile in PGCs using RT-qPCR. We found that on Day 0, immediately after thawing, the cell number in cell lines frozen with the FAM2 medium was significantly higher than in the FAM1-treated ones. On Day 1 and Day 7, the cell number and viability were also higher in most cell lines frozen with FAM2, but the difference was insignificant. The freezing also affected the chicken vasa homologue gene expression in male lines treated with both freezing media.
Effects of Neuromuscular Training on Physical Performance in Older People: A Systematic Review
Authors: Concha-Cisternas Yeny , Castro-Piñero José , Leiva-Ordóñez Ana María , Valdés-Badilla Pablo , Celis-Morales Carlos , Guzmán-Muñoz Eduardo
Published: March 2023

This systematic review aimed to assess the available evidence on the effects of neuromuscular training on physical performance in older adults. A literature search was conducted across four databases (Psychology and Behavioral (EBSCO), Scopus, Web of Science and PubMed). The PRISMA guidelines were followed. The PEDro scale and Cochrane risk of bias tool were used to assess the quality of and risk of bias in the studies, respectively. The protocol was registered in PROSPERO (code: CRD42022319239). The outcomes were muscle strength, cardiorespiratory fitness, postural balance and gait speed. From 610 records initially found, 10 were finally included in the systematic review, involving 354 older people with a mean age of 67.3 years. Nine of them reported significant changes in at least one variable related to physical performance in the intervention compared to the control groups. The neuromuscular training caused significant improvements in postural balance, flexibility, cardiorespiratory fitness, strength power of the upper and lower limbs and autonomy. The available evidence indicates that neuromuscular training has a positive effect on some variables of physical performance, especially in postural balance; however, the methodological quality and certainty of the evidence in the available literature are limited. Therefore, a greater number of high-quality studies are required to draw definitive conclusions.
Seroprevalence of Anti-Echinococcus granulosus Antibodies and Risk Factors for Infection in Blood Donors from Western Romania
Authors: Paduraru Ana Alexandra , Lupu Maria Alina , Lighezan Rodica , Pavel Radu , Cretu Octavian Marius , Olariu Tudor Rares
Published: March 2023

Cystic echinococcosis is a worldwide-distributed zoonotic parasitic disease. This cross-sectional study aimed to assess the seroprevalence and risk factors potentially associated with Echinococcus granulosus in healthy blood donors from Timis County, an endemic region in Western Romania. Serum samples were collected from 1347 Romanian blood donors. Serologic tests to determine the presence of anti-Echinococcus antibodies were performed using an anti-Echinococcus-ELISA immunoassay. Anti-Echinococcus antibodies were detected in 38 blood donors, indicating an overall seroprevalence of 2.8%. The seropositivity rate was 3.7% in females and 3.1% in blood donors residing in urban areas. The highest seropositivity was found in the age group of 31–40 years (3.6%). There were no significant differences between Echinococcus seropositivity and gender, area of residence, age, contact with dogs, or raising sheep. This serologic survey evaluated for the first time the presence of Echinococcus antibodies in healthy blood donors from Western Romania and the potential risk factors associated with echinococcosis. Our results suggest that this zoonotic infection might evolve asymptomatically in apparently healthy individuals. Further studies should be conducted in the general population to estimate the true extent of human echinococcosis and its risk factors.
Evolution and Biogeography, and the Systems Measurement of Mammalian Biotas
Authors: Smith Charles H. , Georges Patrick , Nguyen Ngoc
Published: March 2023

Biological evolution is generally regarded as a stochastic or probabilistic process, per the ideas of Darwin in the nineteenth century. Even if this is true at the meso-scale, it still may, however, be impacted by overarching constraints that we have not yet identified. In this paper, we revisit the subject of mammal faunal regions with a mind to explore a potential kind of macroevolutionary influence. We first identify an optimum seven-region mammal faunal classification system based on spatial and phylogenetic data from a comprehensive 2013 review, and then examine the possibility that this classification provides supporting evidence for a Spinoza-influenced philosophical/theoretical model of the “natural system” concept developed by one of the authors in the 1980s. The hierarchical pattern of regional affinities revealed does do this.
Characterization of 5356 Aluminum Walls Produced by Wire Arc Additive Manufacturing (WAAM)
Authors: Wieczorowski Michal , Pereira Alejandro , Carou Diego , Gapinski Bartosz , Ramírez Ignacio
Published: March 2023

Wire arc additive manufacturing (WAAM) is renowned for its high deposition rate, enabling the production of large parts. However, the process has challenges such as porosity formation, residual stresses, and cracking when manufacturing aluminum parts. This study focuses on ana-lyzing the porosity of AA5356 walls manufactured using the WAAM process with the Fronius cold metal transfer system (Wels, Austria). The walls were machined to obtain specimens for tensile testing. The study used computed tomography and the tensile test to analyze the specimens’ porosity and its potential relation to tensile strength. The process parameters analyzed were travel speed, cooling time, and path strategy. In conclusion, increasing travel speed and cooling time significantly affects pore diameter due to the lower heat input to the weld zone. Porosity can be reduced when diminishing heat accumulation. The results indicate that an increase in travel speed produces a slight decrease in porosity. Specifically, the total pore volume diminishes from 0.42 to 0.36 mm3 when increasing the travel speed from 700 to 950 mm/min. The ultimate tensile strength and maximum elongation of the ‘back and forth’ strategy are slightly higher than those of the ‘go’ strategy. After tensile testing, the ultimate tensile strength and yield strength did not show any relation to the porosity measured by computed tomography. The percentage of the pore total volume over the measured volume was lower than 0.12% for all the scanned specimens.
Shear Bond Strength and Fluoride Release of a Universal Adhesive: An In-Vitro Study on Primary Teeth
Authors: Alsaadawi Alaa , Felemban Osama , Nassar Hani M. , Abdelbaki Medhat
Published: March 2023

This investigation aimed to assess the shear bond strength and fluoride-releasing capabilities of Clearfil Universal Bond Quick (Kuraray Noritake Dental Inc., Tokyo, Japan). Forty-four extracted primary molars were divided into two groups, and the enamel substrate was prepared for evaluating shear bond strength. Scotchbond (3M ESPE) and Clearfil UBQ were used to bond composite-to-enamel substrates in each group (n = 22). Shear bond strength was measured using a universal testing device and compared. Sixteen discs (6 mm diameter and 3 mm thickness) were fabricated from each Clearfil UBQ, Fuji IX, and Fuji II LC. Over the course of 30 days, each materials’ fluoride release was examined and compared using ion analysis. Results revealed that Clearfil UBQ had statistically similar shear bond strength to Scotchbond. Between the three materials, Clearfil UBQ had the lowest fluoride release at baseline (0.11 ± 0.25) and the lowest cumulative fluoride release (0.12–0.27 ppm) over 30 days. Fuji IX had the highest fluoride release at baseline (19.38 ± 2.50) and cumulatively (40.87 ± 4.03 ppm), followed by Fuji II LC. We conclude that Clearfil UBQ and Scotchbond showed comparable bond strengths to the enamel. Fluoride release was seen in Clearfil UBQ in the initial two days of the 30-day period. The amount of fluoride release was significantly less than with glass ionomer cements.
Photothermal Effect and Phase Transition in VO2 Enhanced by Plasmonic Particles
Authors: Kaydashev Vladimir , Khlebtsov Boris , Kutepov Maxim , Nikolskiy Anatoliy , Kozakov Alexey , Konstantinov Alexey , Mikheykin Alexey , Karapetyan Gevork , Kaidashev Evgeni
Published: March 2023

Phase change metasurfaces based on VO2, which are pre-heated with electric current and optically addressed by projected structured light hologram, are considered to become a new paradigm in programmed THz/middle IR flat optics. Macroscopic quasi-homogeneous arrays of Au nanoparticles show large near IR absorption and a significant photothermal effect capable of boosting a light-triggered switching of VO2 and are to be carefully examined. We propose a new approach to simultaneously probe the altered temperature and electric conductivity of a hybrid Au particle-VO2 film composite by monitoring a phase shift and attenuating a surface acoustic wave in a YX128° cut LiNbO3 substrate. The method shows a temperature resolution of 0.1 °C comparable with the best existing techniques for studying nanoobjects and surfaces. The laser-induced photothermal effects were characterized in a macroscopic array of Au nanostars (AuNSts) with different surface coverage. In a monolayer of 10 nm Au, coupled plasmonic nanoparticles were deposited on the LiNbO3 substrate. An optically triggered insulator-metal transition assisted by photothermal effect in AuNSts/VO2/TiO2/LiNbO3 composites was studied at varied light power. We believe that the proposed SAW-based method is of significant importance for the characterization and optimization of radiation absorbing or/and electrically heated elements of metasurfaces and other devices for lab-on-chip and optical communication/processor technology.
Special Issue “Advanced Engineering Cementitious Composites and Concrete Sustainability”
Authors: Burduhos-Nergis Dumitru Doru
Published: March 2023

Concrete, one of the most often-used building materials today, is the cornerstone of modern buildings all over the world, being used for foundations, pavements, building walls, architectural structures, highways, bridges, overpasses, and so on [...]
Study on Kinetics of Carbonization Reaction of Hardened Cement Paste Powder Based on Carbonization Degree
Authors: Zhu Chenhui , Jiang Yibo , Shang Qizhi , Ye Yuchen , Yang Jie
Published: March 2023

The hardened cement paste powder (HCP) powder, devoid of the hydration cementing property, can be regenerated and cemented into a test block with practical strength of almost 60 MPa via CO2 carbonization using appropriate means. This study established a kinetic model of CO2 curing of an HCP powder test block based on the degree of carbonization to study the carbonization reaction kinetic characteristics of the test block. The model was modified according to the characteristics of the evident temperature differences in the reaction kettle in the early, middle, and late stages of the carbonization process. The proposed model can be used to formulate and control the carbonization and cementation processes of HCP powder and can also be applied to describe the kinetics of the reaction processes of other similar systems.
A Comparative Study in the Design of TiO2 Assisted Photocatalytic Coatings Monitored by Controlling Hydrophilic Behavior and Rhodamine B Degradation
Authors: Sandua Xabier , Rivero Pedro J. , Conde Ana , Esparza Joseba , Rodríguez Rafael
Published: March 2023

This work presents a comparative study related to the photocatalytic efficiency associated with wettability measurements and organic dye degradation, as well as other relevant properties (i.e., corrosion resistance, roughness, wettability, and adhesion to a substrate). The photocatalytic precursors are titanium dioxide nanoparticles (TiO2 NPs) which are dispersed onto a polymeric electrospun fiber matrix by using three different deposition techniques such as electrospraying, spraying, and dip-coating, respectively. In this work, the host electrospun matrix is composed of poly(acrylic acid) fibers crosslinked with cyclodextrin (β-CD), which shows a good chemical affinity and stability with the other deposition techniques which are responsible for incorporating the TiO2 NPs. In order to evaluate the efficacy of each coating, the resultant photocatalytic activity has been monitored by two different tests. Firstly, the reduction in the water contact angle is appreciated, and secondly, the degradation of an organic dye (Rhodamine B) is observed under UV irradiation. In addition, the final roughness, adherence, and pitting corrosion potential have also been controlled in order to determine which solution provides the best combination of properties. Finally, the experimental results clearly indicate that the presence of TiO2 NPs deposited by the three techniques is enough to induce a super hydrophilic behavior after UV irradiation. However, there are notable differences in photocatalytic efficiency on the Rhodamine B as a function of the selected deposition technique.
Seawater Biodegradable Poly(butylene succinate-co-adipate)—Wheat Bran Biocomposites
Authors: Strangis Giovanna , Rossi Damiano , Cinelli Patrizia , Seggiani Maurizia
Published: March 2023

The present work focused on the development and characterization of biocomposites based on a fully bio-based polyester, poly(butylene succinate-co-butylene adipate) (PBSA), and wheat bran derived by flour milling. PBSA-bran composites containing 5, 10, 15, and 20 wt.% of wheat bran were produced via melt extrusion and processed by injection molding. Their thermal, rheological, morphological, and tensile properties were investigated. In addition, a biodegradation test in a natural marine environment was conducted on composite dog-bones to assess the capacity of the used filler to increase the PBSA biodegradation rate. The composites maintained similar melt processability and mechanical properties to virgin PBSA with up to 15 wt.% bran content. This result was also supported by morphological investigation, which showed good filler dispersion within the polymer matrix at low-mid bran content, whereas poor polymer-filler dispersion occurred at higher concentrations. Furthermore, the biodegradation tests showed bran’s capacity to improve the PBSA biodegradation rate, probably due to the hygroscopic bran swelling, which induced the fragmentation of the dog-bone with a consequent increase in the polymeric matrix–seawater interfacial area, accelerating the degradation mechanisms. These results encourage the use of wheat bran, an abundant and low-cost agri-food by-product, as a filler in PBSA-based composites to develop products with good processability, mechanical properties, and controlled biodegradability in marine environments.
A Methodology for Predicting the Phase Fraction and Microhardness of Welded Joints Using Integrated Models
Authors: Song Ji-Hyo , Yi Kyung-Woo
Published: March 2023

Understanding the phase transformation and fraction affected by thermal changes is imperative for ensuring the safety of a welded joint. This study proposes a methodology for predicting the phase transformation and fraction of a welded joint using an integrated model. The integrated model includes a heat transfer model and procedures for predicting phase fraction and microhardness. The heat transfer model was developed to simulate the heat transfer in a welded joint and obtain the thermal cycles. The procedure consists of obtaining the peak temperature, austenite fraction, prior austenite grain size (PAGS), and t8/5 (the cooling time between 800 and 500 °C). A database was constructed based on the continuous cooling transformation (CCT) diagram using PAGS and t8/5 as the variables. The phase fraction was then predicted by considering the PAGS with t8/5 from the database. The predicted phase fraction and microhardness were in good agreement with those determined experimentally, demonstrating the reliability of the methodology. This methodology provides a more realistic understanding of phase transformation and facilitates the prediction of the phase fraction and microhardness under various welding conditions that have experimental limitations.
Design and Experimental Validation of a Rapidly Deployable Folding Floating Bridge Based on Rigid-Flexible Combination
Authors: Wang Chenxin , Hu Haiyue , Gan Jin
Published: March 2023

As a temporary means of water transportation, floating bridges play an important role in the military and other fields. However, traditional floating bridges have limitations such as large size, heavy weight, and slow construction time. In this paper, we propose a rigid-flexible composite folding floating bridge. The main structure of the floating bridge consists of three layers: the bridge deck, airbag, and water bag. The floating bridge units are connected by flexible connectors to allow for pre-connection and folding of the bridge, reducing storage and transportation space, and improving construction efficiency. The proposed floating bridge also has a complete engineering application design and has been checked for safety and reliability (including the strength, buoyancy, and bearing capacity of the connections). We used AQWA software to simulate and analyze the anchorage scheme of the floating bridge and its response to wave loads and conducted a ballast test on a floating bridge model to verify its feasibility as a main bearing body. The results show that the floating bridge we designed has the advantages of being lightweight, having fewer consumables, having a small storage and transportation space, and being able to be constructed quickly.
Multi-Step Ahead Ex-Ante Forecasting of Air Pollutants Using Machine Learning
Authors: Gocheva-Ilieva Snezhana , Ivanov Atanas , Kulina Hristina , Stoimenova-Minova Maya
Published: March 2023

In this study, a novel general multi-step ahead strategy is developed for forecasting time series of air pollutants. The values of the predictors at future moments are gathered from official weather forecast sites as independent ex-ante data. They are updated with new forecasted values every day. Each new sample is used to build- a separate single model that simultaneously predicts future pollution levels. The sought forecasts were estimated by averaging the actual predictions of the single models. The strategy was applied to three pollutants—PM10, SO2, and NO2—in the city of Pernik, Bulgaria. Random forest (RF) and arcing (Arc-x4) machine learning algorithms were applied to the modeling. Although there are many highly changing day-to-day predictors, the proposed averaging strategy shows a promising alternative to single models. In most cases, the root mean squared errors (RMSE) of the averaging models (aRF and aAR) for the last 10 horizons are lower than those of the single models. In particular, for PM10, the aRF’s RMSE is 13.1 vs. 13.8 micrograms per cubic meter for the single model; for the NO2 model, the aRF exhibits 21.5 vs. 23.8; for SO2, the aAR has 17.3 vs. 17.4; for NO2, the aAR’s RMSE is 22.7 vs. 27.5, respectively. Fractional bias is within the same limits of (−0.65, 0.7) for all constructed models.
Kinetics Study on the Hydrogen Reduction of Bauxite Residue-Calcite Sintered Pellets at Elevated Temperature
Authors: Kar Manish Kumar , Eijk Casper van der , Safarian Jafar
Published: March 2023

In this study, the isothermal reduction of bauxite residue-calcite sintered pellets by hydrogen at elevated temperatures and different gas flow rates was investigated. A thermogravimetric technique was applied to study the kinetics of the direct reduction by H2 at 500–1000 °C. It was observed that iron in sintered oxide pellets mainly exists in the form of brownmillerite, srebrodolskite and fayalite. The reduction of brownmillerite, the dominant Fe-containing phase, with hydrogen produces mayenite, calcite and metallic iron. X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray fluorescence (XRF), BET surface area, pycnometer and mercury intrusion porosimeter analyses were adopted on reduced pellets to interpret the experimental results. The order of the reduction process changes from first-order reaction kinetics to second-order with an increasing reduction temperature. The change in reaction order may be due to sintering at higher reduction temperatures and corresponding physical and microstructural changes in pellets. The activation energy of reduction was calculated as 55.1–96.6 kJ/mol based on the experimental conditions and using different kinetic model equations. From the experimental observations, it was found that 1000 °C with 60 min is the most suitable condition for bauxite residue-CaO sintered pellets’ reduction with hydrogen.
Heterogeneous Multi-Material Flexible Piezoresistive Sensor with High Sensitivity and Wide Measurement Range
Authors: Yu Tingting , Tao Yebo , Wu Yali , Zhang Dongguang , Yang Jiayi , Ge Gang
Published: March 2023

Flexible piezoresistive sensors (FPSs) have the advantages of compact structure, convenient signal acquisition and fast dynamic response; they are widely used in motion detection, wearable electronic devices and electronic skins. FPSs accomplish the measurement of stresses through piezoresistive material (PM). However, FPSs based on a single PM cannot achieve high sensitivity and wide measurement range simultaneously. To solve this problem, a heterogeneous multi-material flexible piezoresistive sensor (HMFPS) with high sensitivity and a wide measurement range is proposed. The HMFPS consists of a graphene foam (GF), a PDMS layer and an interdigital electrode. Among them, the GF serves as a sensing layer, providing high sensitivity, and the PDMS serves as a supporting layer, providing a large measurement range. The influence and principle of the heterogeneous multi-material (HM) on the piezoresistivity were investigated by comparing the three HMFPS with different sizes. The HM proved to be an effective way to produce flexible sensors with high sensitivity and a wide measurement range. The HMFPS-10 has a sensitivity of 0.695 kPa−1, a measurement range of 0–14,122 kPa, fast response/recovery (83 ms and 166 ms) and excellent stability (2000 cycles). In addition, the potential application of the HMFPS-10 in human motion monitoring was demonstrated.
Flexible and Small Textile Antenna for UWB Wireless Body Area Network
Authors: Chen Peng , Wang Dan , Gan Zongsheng
Published: March 2023

In this paper, a miniaturized textile microstrip antenna is proposed for wireless body area networks (WBAN). The ultra-wideband (UWB) antenna used a denim substrate to reduce the surface wave losses. The monopole antenna consists of a modified circular radiation patch and an asymmetric defected ground structure, which expands impedance bandwidth (BW) and improves the radiation patterns of the antenna with a small size of 20 × 30 × 1.4 mm3. An impedance BW of 110% (2.85–9.81 GHz) frequency boundaries was observed. Based on the measured results, a peak gain of 3.28 dBi was analyzed at 6 GHz. The SAR values were calculated to observe the radiation effects, and the SAR values obtained from the simulation at 4/6/8 GHz frequencies followed the FCC guideline. Compared to typical wearable miniaturized antennas, the antenna size is reduced by 62.5%. The proposed antenna has good performance and can be integrated on a peaked cap as a wearable antenna for indoor positioning systems.
Identification of the Position of a Tethered Delivery Catheter to Retrieve an Untethered Magnetic Robot in a Vascular Environment
Authors: Lee Serim , Kim Nahyun , Kwon Junhyoung , Jang Gunhee
Published: March 2023

In this paper, we propose a method of identifying the position of a tethered delivery catheter in a vascular environment, recombining an untethered magnetic robot (UMR) to the tethered delivery catheter, and safely retrieving them from the vascular environment in an endovascular intervention by utilizing a separable and recombinable magnetic robot (SRMR) and a magnetic navigation system (MNS). From images of a blood vessel and a tethered delivery catheter taken from two different angles, we developed a method of extracting the position of the delivery catheter in the blood vessel by introducing dimensionless cross-sectional coordinates. Then, we propose a retrieval method for the UMR by using the magnetic force considering the delivery catheter’s position, suction force, and rotating magnetic field. We used thane MNS and feeding robot to simultaneously apply magnetic force and suction force to the UMR. In this process, we determined a current solution for generating magnetic force by using a linear optimization method. Finally, we conducted in vitro and in vivo experiments to verify the proposed method. In the in vitro experiment, which was in a glass tube environment, by using an RGB camera, we confirmed that the location of the delivery catheter in the glass tube could be recognized within an average error of 0.05 mm in each of the X- and Z-coordinates and that the retrieval success rate was greatly improved in comparison with that in the case without the use of magnetic force. In an in vivo experiment, we successfully retrieved the UMR in the femoral arteries of pigs.
Assessing the Sensitivity of Plasmopara halstedii Isolates to Mefenoxam through Host Responses
Authors: Nisha Nisha , Vinogradov Sergey , Körösi Katalin , Berisha Arbnora , Bán Rita
Published: March 2023

Downy mildew caused by Plasmopara halstedii is responsible for significant economic losses in cultivated sunflowers. Field isolates of sunflower downy mildew resistant to mefenoxam, a previously effective active ingredient against the pathogen, have been found across Europe. The main goal of this study was to assess the sensitivity of P. halstedii isolates to mefenoxam through host responses to infection, such as symptoms measured by disease severity and growth reduction, and host tissue reactions, such as hypersensitive reaction and necrosis of invaded cells. Sunflower seeds were treated with Apron XL 350 FS at the European registered rate (3 mg/kg seeds). Seedlings were inoculated using the soil drench method with eight Hungarian P. halstedii isolates. Disease rates and plant heights were measured twice. Histological examinations of cross-sections of sunflower hypocotyls were performed using a fluorescence microscope. In our study, cluster analyses of sunflowers based on macroscopic and microscopic variables showed differentiation of groups of mefenoxam-treated sunflowers inoculated with different P. halstedii isolates. We first revealed a clear difference in host responses of mefenoxam-treated susceptible sunflowers. In addition, examining tissue reactions (e.g., hypersensitive reaction, necrosis) seems more accurate to estimate the sensitivity of P. halstedii isolates to mefenoxam than macroscopic symptoms.
The Epichloë festucae Antifungal Protein Efe-AfpA Has Activity against Numerous Plant Pathogens
Authors: Fardella Patrick A. , Clarke Bruce B. , Belanger Faith C.
Published: March 2023

Fungal plant pathogens can present major problems for most crop species. Currently, control of fungal diseases relies heavily on the use of fungicides. However, there are problems associated with fungicide use, including potential toxicity to non-target organisms and the development of resistance in the target fungus. New strategies are being sought to reduce fungicide use. One area of active research is the potential use of antifungal proteins from various fungal species as alternatives or complements to traditional fungicides. An antifungal protein, Efe-AfpA, from the fungal endophyte Epichloë festucae was previously found to protect plants from the pathogen Clarireedia jacksonii, the causal agent of dollar spot disease. Here we report that Efe-AfpA also has inhibitory activity against other important plant pathogens. These results suggest that it may be possible to develop Efe-AfpA as a biofungicide to target a broad range of destructive plant pathogens.
Absence of Hepatitis E Virus (HEV) Circulation in the Most Widespread Wild Croatian Canine Species, the Red Fox (Vulpes vulpes) and Jackal (Canis aureus moreoticus)
Authors: Prpić Jelena , Kunić Ana , Keros Tomislav , Lojkić Ivana , Brnić Dragan , Jemeršić Lorena
Published: March 2023

Hepatitis E virus (HEV) can infect a wide range of domestic and wild animals, and the identification of new host species is reported successively worldwide. Nevertheless, its zoonotic potential and natural transmission, especially in wildlife remains unclear, primarily due to the discrete nature of HEV infections. Since the red fox (Vulpus vulpus) is the most widespread carnivore worldwide, and has been recognized as a potential HEV reservoir, its role as a potent host species is of increasing interest. Another wild canine species, the jackal （Canis aureus moreoticus）, is becoming more important within the same habitat as that of the red fox since its number and geographical distribution have been rapidly growing. Therefore, we have chosen these wild species to determine their potential role in the epidemiology and persistence of HEV in the wilderness. The main reason for this is the finding of HEV and a rather high HEV seroprevalence in wild boars sharing the same ecological niche as the wild canine species, as well as the risk of the spread of HEV through red foxes into the outskirts of cities, where possible indirect and even direct contact with people are not excluded. Therefore, our study aimed to investigate the possibility of natural HEV infection of free-living wild canines, by testing samples for the presence of HEV RNA and anti-HEV antibodies to gain better epidemiological knowledge of the disease. For this purpose, 692 red fox and 171 jackal muscle extracts and feces samples were tested. Neither HEV RNA nor anti-HEV antibodies were detected. Although HEV circulation was not detected in the tested samples, to our knowledge, these are the first results that include jackals as a growing and important omnivore wildlife species for the presence of HEV infection in Europe.
Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques
Authors: Khelaifia Saber , Virginie Pilliol , Belkacemi Souad , Tassery Herve , Terrer Elodie , Aboudharam Gérard
Published: March 2023

Recent years have been marked by a paradigm shift in the study of the human microbiota, with a re-emergence of culture-dependent approaches. Numerous studies have been devoted to the human microbiota, while studies on the oral microbiota still remain limited. Indeed, various techniques described in the literature may enable an exhaustive study of the microbial composition of a complex ecosystem. In this article, we report different methodologies and culture media described in the literature that can be applied to study the oral microbiota by culture. We report on specific methodologies for targeted culture and specific culture techniques and selection methodologies for cultivating members of the three kingdoms of life commonly found in the human oral cavity, namely, eukaryota, bacteria and archaea. This bibliographic review aims to bring together the various techniques described in the literature, enabling a comprehensive study of the oral microbiota in order to demonstrate its involvement in oral health and diseases.
Features of the Physical-Mechanical Properties and Chemical Composition of Chert Gravels
Authors: Tzibulsky Mark , Frid Vladimir
Published: March 2023

The chert gravels are a by-product of sand mining in the south of Israel; the reserves amount to tens of millions of tons and continue to grow. The attempts of their comminution for the production of aggregates by conventional mechanical methods have not yet been successful due to the high abrasiveness, as well as the flaky form of their fracturing. This study was motivated by the need to find an alternative method to rock comminution that would ensure aggregate production in line with the requirements of the asphalt and concrete industry. This article deals with the first inevitable stage on the way to this goal, which consists of an extensive laboratory study of the physical and mechanical properties of the chert gravels, as well as the features of their chemical composition. The results show that the chert rock, consisting of quartz micro grains, contains calcium, sulfur, phosphorus, and barium impurities. The rock is characterized by extremely low porosity and water absorption (less than 1%) and high values of tensile strength (10.8 ± 3.3) and electrical resistivity (23.0 ± 11.9 kΩm). The cubic uniaxial compression strength of the rock is relatively not high (37.3 ± 10.4 MPa), which contradicts the assessment made based on the Schmidt hammer and Point Load studies (158 ± 30.4 MPa and 321 ± 118.5 MPa, respectively).
Viability of Morisca Powder Tailings for Ceramic Applications
Authors: Carvalho Jr. Antônio Alves de , Leite Kelson de Sousa , Elias de Matos José Milton
Published: March 2023

In Juazeiro de Piauí and Castelo do Piauí, two cities in northeast Brazil, mined morisca rock is widely used in civil construction for external coating applications. Aiming at a sustainable practice for its extraction, which includes the economical use of the tailings from its mining, this work presents a solution for the use of discarded materials from processing morisca from Piauí. In this context, an analysis of the results of using morisca as a substitute for clay materials for applications in red ceramics are presented. For the analyses, XRF, XRD, microgranulometry, limits of consistency, TG, DTG, and a set of technological tests inherent to ceramic materials were used. It was concluded that morisca powder is viable as a clay substitute for red ceramic applications.
Structure Features and Physicochemical Performances of Fe-Contained Clinoptilolites Obtained via the Aqueous Exchange of the Balanced Cations and Isomorphs Substitution of the Heulandite Skeletons for Electrocatalytic Activity of Oxygen Evolution Reaction and Adsorptive Performance of CO2
Authors: Wan Chunlei , Cui Xueqing , Liu Ming , Xu Bang , Sun Jihong , Bai Shiyang
Published: March 2023

Fe(III)-modified clinoptilolites (Fe-CPs) were prepared by hydrothermal treatment. The collapse of the heulandite skeletons was avoided by adjusting the pH value using HCl solution, showing the maximum relative crystallinity of the Fe-CPs at an optimal pH of 1.3. The competitive exchange performances between Fe3+ ions and H+ with Na+ (and K+) suggested that the exchange sites were more easily occupied by H+. Various characterizations verified that the hydrothermal treatments had a strong influence on the dispersion and morphology of the isolated and clustered Fe species. The high catalytic activity of the oxygen evolution reaction indicated the insertion of Fe3+ into the skeletons and the occurrences of isomorphic substitution. The fractal evolutions revealed that hydrothermal treatments with the increase of Fe content strongly affected the morphologies of Fe species with rough and disordered surfaces. Meanwhile, the Fe(III)-modified performances of the CPs were systematically investigated, showing that the maximum Fe-exchange capacity was up to 10.6 mg/g. Their thermodynamic parameters and kinetic performances suggested that the Fe(III)-modified procedures belonged to spontaneous, endothermic, and entropy-increasing behaviors. Finally, their adsorption capacities of CO2 at 273 and 298 K were preliminarily evaluated, showing high CO2 adsorption capacity (up to 1.67 mmol/g at 273 K).
Mutated Isocitrate Dehydrogenase (mIDH) as Target for PET Imaging in Gliomas
Authors: Neumaier Felix , Zlatopolskiy Boris D. , Neumaier Bernd
Published: March 2023

Gliomas are the most common primary brain tumors in adults. A diffuse infiltrative growth pattern and high resistance to therapy make them largely incurable, but there are significant differences in the prognosis of patients with different subtypes of glioma. Mutations in isocitrate dehydrogenase (IDH) have been recognized as an important biomarker for glioma classification and a potential therapeutic target. However, current clinical methods for detecting mutated IDH (mIDH) require invasive tissue sampling and cannot be used for follow-up examinations or longitudinal studies. PET imaging could be a promising approach for non-invasive assessment of the IDH status in gliomas, owing to the availability of various mIDH-selective inhibitors as potential leads for the development of PET tracers. In the present review, we summarize the rationale for the development of mIDH-selective PET probes, describe their potential applications beyond the assessment of the IDH status and highlight potential challenges that may complicate tracer development. In addition, we compile the major chemical classes of mIDH-selective inhibitors that have been described to date and briefly consider possible strategies for radiolabeling of the most promising candidates. Where available, we also summarize previous studies with radiolabeled analogs of mIDH inhibitors and assess their suitability for PET imaging in gliomas.
Cannabis Extraction Technologies: Impact of Research and Value Addition in Latin America
Authors: Suárez-Jacobo Ángela , Díaz Pacheco Adrián , Bonales-Alatorre Edgar , Castillo-Herrera Gustavo Adolfo , García-Fajardo Jorge Alberto
Published: March 2023

The Cannabis genus of plants has been widely used in different cultures for various purposes. It is separated into three main species: sativa, indica, and ruderalis. In ancient practices, the plant was used as a multipurpose crop and valued for its fiber, food, and medicinal uses. Since methodologies for the extraction, processing, and identification of components have become available, medical, and food applications have been increasing, allowing potential development in the pharmaceutical and healthy functional food industries. Although the growing legalization and adoption of cannabis for the treatment of diseases are key factors pushing the growth of its market, the biggest challenge is to obtain higher-quality products in a time- and cost-effective fashion, making the process of extraction and separation an essential step. Latin American countries exhibit great knowledge of extraction technologies; nevertheless, it is still necessary to verify whether production costs are economically profitable. In addition, there has been an increase in commercial cannabis products that may or may not be allowed, with or without quality fact sheets, which can pose health risks. Hence, legalization is mandatory and urgent for the rest of Latin American countries. In this article, the phytochemical compounds (cannabinoids, terpenes, and phenolic compounds), the current status of legalization, extraction techniques, and research advances in cannabis in Latin America are reviewed.
Chemical Profiling and Therapeutic Evaluation of Standardized Hydroalcoholic Extracts of Terminalia chebula Fruits Collected from Different Locations in Manipur against Colorectal Cancer
Authors: Thoithoisana Devi Soibam , Devika Chanu Khaidem , Singh Nameirakpam Bunindro , Chaudhary Sushil Kumar , Keithellakpam Ojit Singh , Singh Kshetrimayum Birla , Mukherjee Pulok K. , Sharma Nanaocha
Published: March 2023

Terminalia chebula Retz. (Fam. Combretaceae), locally called Manahei, is a well-known medicinal plant that grows wildly in Manipur, a Northeastern state of India. It is used as a mild laxative, an anti-inflammatory agent, and a remedy for piles, colds, and ulcers by ethnic communities of the state. The hydroalcoholic extract obtained from four fruit samples of T. chebula collected from different locations in Manipur were analyzed using gas chromatography–mass spectrometry (GC-MS) and high-performance thin-layer chromatography (HPTLC) for their chemical constituents and evaluated for their anticancer activity against the colon cancer cell HCT 116. GC-MS analysis results indicated significant variation in the composition and percentage of major compounds present in the extracts. 1,2,3-Benzenetriol was the most abundant chemical constituent present in all four extracts of T. chebula, ranging from 20.95 to 43.56%. 2-Cyclopenten-1-one, 5-hydroxymethylfurfural, and catechol were commonly present in all extracts. Two marker compounds, gallic acid and ellagic acid, were also quantified usingHPTLC in all four extracts of T. chebula. The highest content of gallic acid (22.44 ± 0.056 µg/mg of dried extract) was observed in TCH, and that of ellagic acidwas found in TYH (11.265 ± 0.089 µg/mg of dried extract). The IC50 value of TYH for the DPPH and ABTS assays (12.16 ± 0.42 and 7.80 ± 0.23 µg/mL) was found to be even lower than that of Trolox (18 ± 0.44 and 10.15 ± 0.24 µg/mL), indicating its strong antioxidant properties among the four extracts of T. chebula. The MTT assay determined the effect of T. chebula extracts on the viability of HCT 116 cells. TYH showed the highest activity with anIC50 value of 52.42 ± 0.87 µg/mL, while the lowest activity was observed in TCH (172.05 ± 2.0 µg/mL). The LDH assay confirmed the cytotoxic effect of TYH in HCT 116 cells. TYH was also found to induce caspase-dependent apoptosis in HCT 116 cells after 48 h of treatment. Our study provides insight into the diversity of T. chebula in Manipur and its potential activity against colon cancer.
New Triterpenoids and Anti-Inflammatory Constituents from Glinus oppositifolius
Authors: Chen Jih-Jung , Yang Chang-Syun , Chen Yu-Hui , Chao Che-Yi , Chen Yu-Chang , Kuo Yeuh-Hsiung
Published: March 2023

Three new triterpenoids—spergulagenin B (1), spergulagenin C (2), and spergulagenin D (3)—were isolated from the aerial part of Glinus oppositifolius, along with 17 known compounds (4–20). The structures of these new compounds were identified by spectroscopic and MS analyses. Compounds 3, 5, 19, and 20 were evaluated for inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells with IC50 values of 17.03, 18.21, 16.30, and 12.64 μM, respectively. Compounds 3, 5, and 20 exhibited inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 cells with IC50 values of 18.35 ± 1.34, 17.56 ± 1.41, and 14.27 ± 1.29 μM, respectively.