bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantum machine learning for image classification
Authors: Senokosov Arsenii , Sedykh Alexandr , Sagingalieva Asel , Kyriacou Basil , Melnikov Alexey
Published: February 2024

Image classification, a pivotal task in multiple industries, faces computational challenges due to the burgeoning volume of visual data. This research addresses these challenges by introducing two quantum machine learning models that leverage the principles of quantum mechanics for effective computations. Our first model, a hybrid quantum neural network with parallel quantum circuits, enables the execution of computations even in the noisy intermediate-scale quantum era, where circuits with a large number of qubits are currently infeasible. This model demonstrated a record-breaking classification accuracy of 99.21% on the full MNIST dataset, surpassing the performance of known quantum–classical models, while having eight times fewer parameters than its classical counterpart. Also, the results of testing this hybrid model on a Medical MNIST (classification accuracy over 99%), and on CIFAR-10 (classification accuracy over 82%), can serve as evidence of the generalizability of the model and highlights the efficiency of quantum layers in distinguishing common features of input data. Our second model introduces a hybrid quantum neural network with a Quanvolutional layer, reducing image resolution via a convolution process. The model matches the performance of its classical counterpart, having four times fewer trainable parameters, and outperforms a classical model with equal weight parameters. These models represent advancements in quantum machine learning research and illuminate the path towards more accurate image classification systems.
Immune checkpoint inhibitor–related colitis in patients on immunotherapy for cancer
Authors: Sun Belinda L , Elliott Alexis S , Nolte David , Sun Xiaoguang
Published: February 2024

Objectives

Immune checkpoint inhibitors, a revolutionary class of cancer immunotherapy drugs, have transformed cancer treatment by bolstering antitumor immunity for various advanced-stage solid cancers. The US Food and Drug Administration has approved 7 immune checkpoint inhibitors that target 3 major immune checkpoint proteins: cytotoxic T-lymphocyte–associated protein 4, programmed cell death 1 protein, and programmed cell death 1 ligand 1. In addition to their remarkable efficacy, however, these inhibitors have been observed causing immune-related adverse events, particularly immune checkpoint inhibitor–related colitis, which often results in severe or life-threatening clinical issues.

Methods

The diagnosis of immune checkpoint inhibitor–related colitis relies on incorporation of clinical evaluation as well as endoscopic and histopathologic examination, with exclusion of other potential etiologies.

Results

The common histopathologic manifestations of immune checkpoint inhibitor–related colitis are acute active colitis, chronic active colitis, microscopic colitis (collagenous or lymphocytic), and ischemic colitis, with patterns overlapping. Notably, enterocyte apoptosis is a unique feature of immune checkpoint inhibitor toxicity. The proposed mechanisms for the pathogenesis of immune checkpoint inhibitor–related colitis are primarily associated with autoimmune-type dysregulation and gut microbiome alteration. This review summarizes the clinical and pathologic characteristics of immune checkpoint inhibitor–related colitis and elucidates its underlying pathogenic mechanisms.

Conclusions

Future successful management of this form of colitis relies on our comprehension of the intricate interplay between tumoral and systemic immune responses to immune checkpoint inhibitors and innovative approaches to modify these responses, along with specific immune cell populations, to preclude immune-related adverse events while achieving antitumor therapeutic outcomes.
A 120-Minute Saline Infusion Test for the Confirmation of Primary Aldosteronism: A Pilot Study
Authors: Golani Tiran , Bleier Jonathan , Kaplan Alon , Hod Tammy , Sharabi Yehonatan , Leibowitz Avshalom , Grossman Ehud , Shlomai Gadi
Published: February 2024

BACKGROUND

The saline infusion test (SIT) to confirm primary aldosteronism requires infusing 2 L of normal saline over 240 minutes. Previous studies raised concerns regarding increased blood pressure and worsening hypokalemia during SIT. We aimed to evaluate the diagnostic applicability of a SIT that requires 1 L of saline infusion over 120 minutes.

METHODS

A cross-sectional study, including all patients in a large medical center who underwent SIT from 1 January 2015 to 30 April 2023. Blood samples were drawn for baseline renin and aldosterone (t = 0) after 2 hours (t = 120 min) and after 4 hours (t = 240 min) of saline infusion. We used ROC analysis to evaluate the sensitivity and specificity of various aldosterone cut-off values at t = 120 to confirm primary aldosteronism.

RESULTS

The final analysis included 62 patients. A ROC analysis yielded 97% specificity and 90% sensitivity for a plasma aldosterone concentration (PAC) of 397 pmol/L (14 ng/dL) at t = 120 to confirm primary aldosteronism, and an area under the curve of 0.97 (95% CI [0.93, 1.00], P < 0.001). Almost half (44%) of the patients did not suppress PAC below 397 pmol/L (14 ng/dL) at t = 120. Of them, only one (4%) patient suppressed PAC below 276 pmol/L (10 ng/dL) at t = 240. Mean systolic blood pressure increased from 140.1 ± 21.3 mm Hg at t = 0 to 147.6 ± 14.5 mm Hg at t = 240 (P = 0.011).

CONCLUSIONS

A PAC of 397 pmol/L (14 ng/dL) at t = 120 has high sensitivity and specificity for primary aldosteronism confirmation.
Optimizing Implant Width Selection in Breast Augmentation: Insights From On-Patient Landmark Positioning in 3-Dimensional Breast Simulation
Authors: Akgun Demir Isil
Published: February 2024

Background

The Vectra XT 3D simulation program is a valuable tool for implant selection during the decision-making process regarding breast augmentation. The software allows for the assignment of automatically or manually positioned anatomical landmarks and provides accompanying measurements.

Objectives

This study aimed to present a strategy for standardizing landmark placement on Vectra images during primary breast augmentations, optimizing implant selection and ensuring consistency in breast width data collection.

Methods

The author performed a retrospective analysis of patient data in which breast width measurements were obtained through clinical assessment (CA) and the Vectra program with both automatically (VA) and manually (VM) placed landmarks. Consideration was also given to the thickness of medial and lateral breast tissues. The suggested implant base width from all groups was then compared to the actual implant placed during the surgical procedure.

Results

Comparing the change rates with the actual implant width revealed that rates in CA measurements were significantly lower than those in VA (P < .05), and similarly lower than those in VM (P < .05). Furthermore, change rates in VM were significantly lower than in VA (P < .05). Each group showed a significant positive correlation with the actual implant width.

Conclusions

The Vectra XT 3D simulation program is a valuable adjunct in breast augmentation. However, relying on it solely may lead to inaccuracies in the assessment of breast dimensions due to automatic landmarking. To achieve standardized measurements, landmarks can be marked on the patient before creating the Vectra image. Our results underline the superiority of clinical judgment to simulation programs when establishing a reliable surgical plan.

Level of Evidence: 3
IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis
Authors: Li Wei , Li Hangyu , Hu Qiongyao , Wang Linlin , Yin Zhan , Hu Guangfu
Published: February 2024

Reproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus–pituitary–gonad axis could inhibit the development of gonads. These results indicated that fasting may participate in the regulation of fish gonadal development through the mediation of IGFBP1a. Further studies found that IGFBP1a could markedly inhibit gonadotropin-releasing hormone 3 expressions in hypothalamus cells. At the pituitary level, IGFBP1a could significantly reduce the gonadotropin hormones (LH and FSH) expression by blocking the action of pituitary insulin-like growth factor 1. Interestingly, IGFBP1a could also directly inhibit the expression of lhr, fshr, and sex steroid hormone synthase genes (cyp11a, cyp17a, and cyp19a1) in the ovary. These results indicated that IGFBP1a should be a nutrient deficient response factor that could inhibit fish reproduction through the hypothalamus–pituitary–ovary axis.
Recurrence-free survival as a surrogate endpoint for overall survival after neoadjuvant chemotherapy and surgery for oesophageal squamous cell carcinoma
Authors: Okui Jun , Nagashima Kengo , Matsuda Satoru , Sato Yasunori , Okamura Akihiko , Kawakubo Hirofumi , Muto Manabu , Kakeji Yoshihiro , Kono Koji , Takeuchi Hiroya , Watanabe Masayuki , Doki Yuichiro , Bamba Takeo , Fukuda Takashi , Fujiwara Hitoshi , Sato Shinsuke , Noma Kazuhiro , Miyata Hiroshi , Fujita Takeo , Kitagawa Yuko
Published: February 2024

Background

Overall survival is considered as one of the most important endpoints of treatment efficacy but often requires long follow-up. This study aimed to determine the validity of recurrence-free survival as a surrogate endpoint for overall survival in patients with surgically resectable advanced oesophageal squamous cell carcinoma (OSCC).

Methods

Patients with OSCC who received neoadjuvant cisplatin and 5-fluorouracil, or docetaxel, cisplatin and 5-fluorouracil, at 58 Japanese oesophageal centres certified by the Japan Esophageal Society were reviewed retrospectively. The correlation between recurrence-free and overall survival was assessed using Kendall's τ.

Results

The study included 3154 patients. The 5-year overall and recurrence-free survival rates were 56.6 and 47.7% respectively. The primary analysis revealed a strong correlation between recurrence-free and overall survival (Kendall's τ 0.797, 95% c.i. 0.782 to 0.812) at the individual level. Subgroup analysis showed a positive relationship between a more favourable pathological response to neoadjuvant chemotherapy and a higher τ value. In the meta-regression model, the adjusted R2 value at the institutional level was 100 (95% c.i. 40.2 to 100)%. The surrogate threshold effect was 0.703.

Conclusion

There was a strong correlation between recurrence-free and overall survival in patients with surgically resectable OSCC who underwent neoadjuvant chemotherapy, and this was more pronounced in patients with a better response to neoadjuvant chemotherapy.
Staging of progressive supranuclear palsy-Richardson syndrome using MRI brain charts for the human lifespan
Authors: Planche Vincent , Mansencal Boris , Manjon Jose V , Meissner Wassilios G , Tourdias Thomas , Coupé Pierrick
Published: February 2024

Brain charts for the human lifespan have been recently proposed to build dynamic models of brain anatomy in normal aging and various neurological conditions. They offer new possibilities to quantify neuroanatomical changes from preclinical stages to death, where longitudinal MRI data are not available. In this study, we used brain charts to model the progression of brain atrophy in progressive supranuclear palsy—Richardson syndrome. We combined multiple datasets (n = 8170 quality controlled MRI of healthy subjects from 22 cohorts covering the entire lifespan, and n = 62 MRI of progressive supranuclear palsy—Richardson syndrome patients from the Four Repeat Tauopathy Neuroimaging Initiative (4RTNI)) to extrapolate lifetime volumetric models of healthy and progressive supranuclear palsy—Richardson syndrome brain structures. We then mapped in time and space the sequential divergence between healthy and progressive supranuclear palsy—Richardson syndrome charts. We found six major consecutive stages of atrophy progression: (i) ventral diencephalon (including subthalamic nuclei, substantia nigra, and red nuclei), (ii) pallidum, (iii) brainstem, striatum and amygdala, (iv) thalamus, (v) frontal lobe, and (vi) occipital lobe. The three structures with the most severe atrophy over time were the thalamus, followed by the pallidum and the brainstem. These results match the neuropathological staging of tauopathy progression in progressive supranuclear palsy—Richardson syndrome, where the pathology is supposed to start in the pallido-nigro-luysian system and spreads rostrally via the striatum and the amygdala to the cerebral cortex, and caudally to the brainstem. This study supports the use of brain charts for the human lifespan to study the progression of neurodegenerative diseases, especially in the absence of specific biomarkers as in PSP.
White matter network underlying semantic processing: evidence from stroke patients
Authors: Xiao Xiangyue , Dong Zhicai , Yu Mingyan , Ding Junhua , Zhang Maolin , Cruz Sara , Han Zaizhu , Chen Yan
Published: February 2024

The hub-and-spoke theory of semantic representation fractionates the neural underpinning of semantic knowledge into two essential components: the sensorimotor modality-specific regions and a crucially important semantic hub region. Our previous study in patients with semantic dementia has found that the hub region is located in the left fusiform gyrus. However, because this region is located within the brain damage in patients with semantic dementia, it is not clear whether the semantic deficit is caused by structural damage to the hub region itself or by its disconnection from other brain regions. Stroke patients do not have any damage to the left fusiform gyrus, but exhibit amodal and modality-specific deficits in semantic processing. Therefore, in this study, we validated the semantic hub region from a brain network perspective in 79 stroke patients and explored the white matter connections associated with it. First, we collected data of diffusion-weighted imaging and behavioural performance on general semantic tasks and modality-specific semantic tasks (assessing object knowledge on form, colour, motion, sound, manipulation and function). We then used correlation and regression analyses to examine the association between the nodal degree values of brain regions in the whole-brain structural network and general semantic performance in the stroke patients. The results revealed that the connectivity of the left fusiform gyrus significantly predicted general semantic performance, indicating that this region is the semantic hub. To identify the semantic-relevant connections of the semantic hub, we then correlated the white matter integrity values of each tract connected to the left fusiform gyrus separately with performance on general and modality-specific semantic processing. We found that the hub region accomplished general semantic processing through white matter connections with the left superior temporal pole, middle temporal gyrus, inferior temporal gyrus and hippocampus. The connectivity between the hub region and the left hippocampus, superior temporal pole, middle temporal gyrus, inferior temporal gyrus and parahippocampal gyrus was differentially involved in object form, colour, motion, sound, manipulation and function processing. After statistically removing the effects of potential confounding variables (i.e. whole-brain lesion volume, lesion volume of regions of interest and performance on non-semantic control tasks), the observed effects remained significant. Together, our findings support the role of the left fusiform gyrus as a semantic hub region in stroke patients and reveal its crucial connectivity in the network. This study provides new insights and evidence for the neuroanatomical organization of semantic memory in the human brain.
Isolated right ventricular hypoplasia associated with cyanotic atrial septal defect: a case report
Authors: Gohbara Sakiko , Mochizuki Yasuhide , Fujii Takanari , Tomita Hideshi , Shinke Toshiro
Published: February 2024

Background

Hypoxaemia in isolated right ventricular (RV) hypoplasia (IRVH) is primarily caused by a right-to-left shunt (RLS) at the atrial level, such as an atrial septal defect (ASD). When considering closure of the RLS, it should be closed only after ensuring that it will not cause right-sided heart failure (HF).

Case summary

A 21-year-old woman had been experiencing shortness of breath during exertion since childhood. Transthoracic and transoesophageal echocardiography revealed an ASD with bidirectional shunting, and microbubble test revealed a marked RLS. Cardiac magnetic resonance imaging revealed a hypoplastic RV end-diastolic volume corrected for body surface area of 47 mL/m2 (70% of normal range). Right heart catheterization revealed a decreased Qp/Qs ratio of 0.89 and a pressure waveform with a clear increase in the ‘A’-wave, although the mean right atrial pressure was not high (4 mmHg). Therefore, the patient was diagnosed with cyanotic ASD and IRVH. A temporary balloon occlusion test was performed to evaluate the right-sided heart response to capacitive loading prior to ASD closure. After treatment, the patient’s improved markedly. The pre-operative brain natriuretic peptide (BNP) level was normal; however, 6 months after ASD closure, the BNP level was elevated, and the continuous-wave Doppler waveform of pulmonary regurgitation at the time of transthoracic echocardiography changed, suggesting an increase in diastolic RV pressure.

Discussion

When ASD is complicated by hypoxaemia, the possibility of IRVH, although rare, should be considered. Another difficult point is determining whether the ASD can be closed, considering its immature RV compliance.
A missing coronary guidewire mimicking aortic dissection—a case report
Authors: Arow Ziad , Bar Sef Avigdor , Assali Abid , Arnson Yoav
Published: February 2024

Background

A retained coronary guidewire following coronary angiography is an extremely rare complication. We present a case of a retained coronary guidewire from a percutaneous coronary intervention done 2 years ago.

Case summary

An 80-year-old asymptomatic man with a history of ischemic heart disease and moderate aortic stenosis presented to the echocardiography lab for routine follow-up. Transthoracic echocardiography showed Moderate aortic stenosis and a suspected linear echogenic structure in the ascending aorta. trans-esophageal echocardiography was performed to reveal a mobile and linear echogenic structure originating from the sinuses of Valsalva/Sinotubular junction and extending to the ascending aorta. An electrocardiogram gated cardiac computed tomography was performed and showed A linear well-defined structure originating from the ostium of the left main coronary artery and extending to the ascending aorta—a coronary guidewire from an earlier procedure. A second look at the last invasive coronary angiography record demonstrated the same finding. A multidisciplinary heart team discussion was obtained and concluded that the risk of surgical or endovascular intervention outweighed the potential benefit. The patient was discharged home for a close clinical and echocardiographic follow-up.

Discussion

A retained coronary guidewire is a rare complication that operators should be aware of. Management should be case-specific depending on clinical presentation.
Proteome allocation and the evolution of metabolic cross-feeding
Authors: Labourel Florian J F , Daubin Vincent , Menu Frédéric , Rajon Etienne
Published: February 2024

In a common instance of metabolic cross-feeding (MCF), an organism incompletely metabolizes nutrients and releases metabolites that are used by another to produce energy or building blocks. Why would the former waste edible food, and why does this preferentially occur at specific locations in a metabolic pathway have challenged evolutionary theory for decades. To address these questions, we combine adaptive dynamics with an explicit model of cell metabolism, including enzyme-driven catalysis of metabolic reactions and the cellular constraints acting on the proteome that may incur a cost to expressing all enzymes along a pathway. After pointing out that cells should in principle prioritize upstream reactions when metabolites are restrained inside the cell, we show that the occurrence of permeability-driven MCF is rare and requires that an intermediate metabolite be extremely diffusive. Indeed, only at very high levels of membrane permeability (consistent with those of acetate and glycerol, for instance) and under distinctive sets of parameters should the population diversify and MCF evolve. These results help understand the origins of simple microbial communities, such as those that readily evolve in short-term evolutionary experiments, and may later be extended to investigate how evolution has progressively built up today’s extremely diverse ecosystems.
Evaluating the liver abscess microbiota of beef cattle during a reduction in tylosin supplementation shows differences according to abscess size and fraction
Authors: O'Hara Eóin , Zaheer Rahat , Andrés-Lasheras Sara , McAllister Tim A , Gruninger Robert J
Published: February 2024

Liver abscesses (LA) resulting from bacterial infection in cattle pose a significant global challenge to the beef and dairy industries. Economic losses from liver discounts at slaughter and reduced animal performance drive the need for effective mitigation strategies. Tylosin phosphate supplementation is widely used to reduce LA occurrence, but concerns over antimicrobial overuse emphasize the urgency to explore alternative approaches. Understanding the microbial ecology of LA is crucial to this, and we hypothesized that a reduced timeframe of tylosin delivery would alter LA microbiomes. We conducted 16S rRNA sequencing to assess severe liver abscess bacteriomes in beef cattle supplemented with in-feed tylosin. Our findings revealed that shortening tylosin supplementation did not notably alter microbial communities. Additionally, our findings highlighted the significance of sample processing methods, showing differing communities in bulk purulent material and the capsule-adhered material. Fusobacterium or Bacteroides ASVs dominated LA, alongside probable opportunistic gut pathogens and other microbes. Moreover, we suggest that liver abscess size correlates with microbial community composition. These insights contribute to our understanding of factors impacting liver abscess microbial ecology and will be valuable in identifying antibiotic alternatives. They underscore the importance of exploring varied approaches to address LA while reducing reliance on in-feed antibiotics.
Quantitation of human mitochondrial DNA and whole mtGenomes sequencing of fingernail/hair shaft samples
Authors: Li Hui , Cao Yu , Yang Fan , Liu Xiling , Tao Ruiyang , Xia Ruocheng , Zhu Ruxin , Jiang Lei , Liu Shiquan , Li Chengtao
Published: February 2024

The analysis of mitochondrial DNA (mtDNA) is suitable for fingernail/hair shaft samples in forensic science. A successful mtDNA forensic analysis depends on the quantity and quality of the mtDNA. The application of massively parallel sequence techniques to the analysis of mtDNA has the potential to improve the recovery of genetic information from difficult forensic specimens and to increase the discrimination potential of mtDNA by capturing and comparing full mtGenomes. In this study, we constructed mtDNA-specific standard curves using real-time PCR. The 105-base pair target sequence facilitates the amplification of degraded DNA and is minimally homologous to non-human mtDNA. The results indicated that the assay enables the absolute quantification of down to 10 copies of mtDNA and provides a dynamic range of eight orders of magnitude. Whole mtGenome sequencing experiments demonstrated that as few as 2 000 mtDNA copies resulted in a successful full region amplification and sequencing. Moreover, the frequency of point heteroplasmy from one donor showed that hairs from the same donor have been found to differ within and among themselves and from other tissues, which could impact the interpretation of the results obtained in a forensic investigation.
Deciphering spatial domains from spatially resolved transcriptomics with Siamese graph autoencoder
Authors: Cao Lei , Yang Chao , Hu Luni , Jiang Wenjian , Ren Yating , Xia Tianyi , Xu Mengyang , Ji Yishuai , Li Mei , Xu Xun , Li Yuxiang , Zhang Yong , Fang Shuangsang
Published: February 2024

Background

Cell clustering is a pivotal aspect of spatial transcriptomics (ST) data analysis as it forms the foundation for subsequent data mining. Recent advances in spatial domain identification have leveraged graph neural network (GNN) approaches in conjunction with spatial transcriptomics data. However, such GNN-based methods suffer from representation collapse, wherein all spatial spots are projected onto a singular representation. Consequently, the discriminative capability of individual representation feature is limited, leading to suboptimal clustering performance.

Results

To address this issue, we proposed SGAE, a novel framework for spatial domain identification, incorporating the power of the Siamese graph autoencoder. SGAE mitigates the information correlation at both sample and feature levels, thus improving the representation discrimination. We adapted this framework to ST analysis by constructing a graph based on both gene expression and spatial information. SGAE outperformed alternative methods by its effectiveness in capturing spatial patterns and generating high-quality clusters, as evaluated by the Adjusted Rand Index, Normalized Mutual Information, and Fowlkes–Mallows Index. Moreover, the clustering results derived from SGAE can be further utilized in the identification of 3-dimensional (3D) Drosophila embryonic structure with enhanced accuracy.

Conclusions

Benchmarking results from various ST datasets generated by diverse platforms demonstrate compelling evidence for the effectiveness of SGAE against other ST clustering methods. Specifically, SGAE exhibits potential for extension and application on multislice 3D reconstruction and tissue structure investigation. The source code and a collection of spatial clustering results can be accessed at https://github.com/STOmics/SGAE/.
Joint inversion method of multipoint ambient noise horizontal-to-vertical spectral ratio for 3-D velocity structure of local site and its application
Authors: Wang Jixin , Rong Mianshui , Li Xiaojun , Chen Su , Wang Yushi , Zhu Jun
Published: February 2024

The diffusion field theory has been widely used to interpret ambient noise wave fields. Based on this theory, 1-D subsurface velocity structure inversion method is developed. However, few studies have referred to the noise horizontal to vertical (NHV) spectral ratio inversion of 3-D subsurface velocity structures, and almost no effective 3-D NHV inversion tools have been developed. To develop a useful tool for obtaining 3-D soil layer velocity structures, we combined the NHV forward calculation formula derived from diffusion field theory with the guided Monte Carlo algorithm and then extended the single-point NHV inversion to multipoint joint inversion through a joint objective function. Subsequently, a new 3-D soil layer velocity structure inversion method was proposed. Subsequently, a synthetic 2-D case was used to verify the proposed method. Finally, the proposed method was applied to the Xiangtang Array in Tangshan, China, to identify the 3-D velocity structures of the site based on noise observations. The results show that the proposed multipoint joint 3-D inversion method is effective for identifying 3-D underground velocity structures.
Rapid detection of viable microbes with 5-cyano-2,3-di-(p-tolyl)tetrazolium chloride and 5(6)-carboxyfluorescein diacetate using a fibre fluorescence spectroscopy system
Authors: Chiang Jessica , Robertson Julia , McGoverin Cushla M , Swift Simon , Vanholsbeeck Frédérique
Published: February 2024

Aims

To assess the efficacy of two commercially available viability dyes, 5-cyano-2,3-di-(p-tolyl)tetrazolium chloride (CTC) and 5(6)-carboxyfluorescein diacetate (CFDA), in reporting on viable cell concentration and species using an all-fibre fluorometer.

Methods and results

Four bacterial species (two Gram-positive and two Gram-negative) commonly associated with food poisoning or food spoilage (Escherichia coli, Salmonella enterica, Staphylococcus aureus, and Bacillus cereus) were stained with CTC or CFDA and the fibre fluorometer was used to collect full fluorescence emission spectra. A good correlation between concentration and fluorescence intensity was found for Gram-negative bacteria between 107 and 108 colony-forming units (CFU) ml−1. There was no correlation with concentration for Gram-positive bacteria; however, the information in the CTC and CFDA spectra shows the potential to distinguish Gram-negative cells from Gram-positive cells, although it may simply reflect the overall bacterial metabolic activity under staining conditions from this study.

Conclusions

The limit of detection (LoD) is too high in the dip-probe approach for analysis; however, the development of an approach measuring the fluorescence of single cells may improve this limitation. The development of new bacteria-specific fluorogenic dyes may also address this limitation. The ability to differentiate bacteria using these dyes may add value to measurements made to enumerate bacteria using CTC and CFDA.
Julia Keiner’s Universalism and the Question of Israeli style
Authors: Bernstein Noga
Published: February 2024

The existence, or lack thereof, of a distinguishable Israeli style remains a central question in the historiography of Israeli art and design. This a examines tensions produced by this debate between the 1940s and 1960s—a formative period of Israeli nation-building—as manifested in the design and pedagogical ideology of handweaver Julia Keiner (1900–1992), who immigrated to Mandatory Palestine from Germany in 1936 and founded the textile department at the New Bezalel School of Art and Craft. I argue that, for Keiner, good design was based on objective, universal principles stemming from the interrelation of process and material, as well as the objective laws of nature. However, her work was ineluctably entangled with this search for a distinctly Hebrew or Israeli style. This essay shows how each of the textile department’s two fields of training—weaving and embroidery—reflected tensions between Keiner’s universalist approach and local efforts to establish such a national style.
Unraveling the Myotis morass: ultraconserved-element analysis reveals introgression, cryptic diversity, and taxonomic trouble
Authors: Korstian Jennifer M , Stevens Richard D , Lee Thomas E , Baker Robert J , Ray David A
Published: February 2024

Using sequences from 2,615 ultraconserved element (UCE) loci and multiple methodologies we inferred phylogenies for the largest genetic data set of New World bats in the genus Myotis to date. The resulting phylogenetic trees were populated with short branch lengths and widespread conflict, hallmarks consistent with rapid adaptive radiations. The degree of conflict observed in Myotis has likely contributed to difficulties disentangling deeper evolutionary relationships. Unlike earlier phylogenies based on 1 to 2 gene sequences, this UCE data set places M. brandtii outside the New World clades. Introgression testing of a small subset of our samples revealed evidence of historical but not contemporary gene flow, suggesting that hybridization occurs less frequently in the Neotropics than the Nearctic. We identified several instances of cryptic lineages within described species as well as several instances of potential taxonomic oversplitting. Evidence from Central and South American localities suggests that diversity in those regions is not fully characterized. In light of the accumulated evidence of the evolutionary complexity in Myotis and our survey of the taxonomic implications from our phylogenies, it is apparent that the definition of species and regime of species delimitation need to be reevaluated for Myotis. This will require substantial collaboration and sample sharing between geneticists and taxonomists to build a system that is both robust and applicable in a genus as diverse as Myotis.
The Hell With Questions
Authors: Ippolito Michela
Published: February 2024

We discuss previous proposals for the semantics of wh-the-hell questions (domain widening theories and domain restriction theories), highlighting the challenges these accounts face in trying to explain the different properties of wh-the-hell questions and capture the contribution this expression makes to the semantics of the question. We review the semantic properties of wh-the-hell questions discussed in the literature and propose a new analysis according to which the hell signals doxastic dissonance. We argue that this proposal accounts for the semantic properties of this type of expletive question, and has the potential to extend to the class of wh-the-hell questions we see across languages.
A case of image-guided hematometrocolpos drainage requiring tissue plasminogen activator in a pediatric patient
Authors: Sharma Pareena , Shah Jay , Sokkary Nancy
Published: February 2024

Hematometrocolpos (HMC) is a rare disorder that occurs when an anatomical anomaly like imperforate hymen causes menstrual blood to be retained in the uterus and vagina. There is no standard of care established for HMC beyond urgent vaginoplasty which requires a demanding post-operative course that may not be suited for all pediatric patients. This is a case report of successful use of image-guided percutaneous drainage of HMC with tissue plasminogen activator (TPA) followed by vaginoplasty in a 13-year-old female with lower vaginal atresia. Additionally, this case explores the role of menstrual suppression and the need for individualized guidelines. It emphasizes the potential of image-guided percutaneous drainage with TPA as a promising, less-invasive treatment option for pediatric HMC as well as the impact on follow-up surgery.
The cosmic baryon partition between the IGM and CGM in the SIMBA simulations
Authors: Khrykin Ilya S , Sorini Daniele , Lee Khee-Gan , Davé Romeel
Published: February 2024

We use the simba suite of cosmological hydrodynamical simulations to investigate the importance of various stellar and active galactic nuclei (AGN) feedback mechanisms in partitioning the cosmic baryons between the intergalactic (IGM) and circumgalactic (CGM) media in the z ≤ 1 Universe. We identify the AGN jets as the most prominent mechanism for the redistribution of baryons between the IGM and CGM. In contrast to the full feedback models, deactivating AGN jets results in ≈20 per cent drop in fraction of baryons residing in the IGM and a consequent increase of CGM baryon fraction by ≈50 per cent. We find that stellar feedback modifies the partition of baryons on a 10 per cent level. We further examine the physical properties of simulated haloes in different mass bins, and their response to various feedback models. On average, a sixfold decrease in the CGM mass fraction due to the inclusion of feedback from AGN jets is detected in $10^{12}\, {\rm M}_{\odot } \le M_{\rm 200} \le 10^{14}\, {\rm M}_{\odot }$ haloes. Examination of the average radial gas density profiles of $M_{200} \gt 10^{12}\, {\rm M}_{\odot }$ haloes reveals up to an order of magnitude decrease in gas densities due to the AGN jet feedback. We compare gas density profiles from simba simulations to the predictions of the modified Navarro–Frenk–White model, and show that the latter provides a reasonable approximation within the virial radii of the full range of halo masses, but only when rescaled by the appropriate mass-dependent CGM fraction of the halo. The relative partitioning of cosmic baryons and, subsequently, the feedback models can be constrained observationally with fast radio bursts in upcoming surveys.
Pre-perihelion monitoring of interstellar comet 2I/Borisov
Authors: Prodan George P , Popescu Marcel , Licandro Javier , Akhlaghi Mohammad , de León Julia , Tatsumi Eri , Pastrav Bogdan Adrian , Hibbert Jacob M , Vǎduvescu Ovidiu , Simion Nicolae Gabriel , Pallé Enric , Narita Norio , Fukui Akihiko , Murgas Felipe
Published: February 2024

The discovery of interstellar comet 2I/Borisov offered the unique opportunity to obtain a detailed analysis of an object coming from another planetary system, and leaving behind material in our interplanetary space. We continuously observed 2I/Borisov between 2019 October 3 and December 13 using the 1.52-m Telescopio Carlos Sánchez equipped with Multicolour Simultaneous Camera for studying Atmospheres of Transiting exoplanets 2 instrument, and the 2.54-m Isaac Newton Telescope with Wide Field Camera. We characterize its morphology and spectro-photometric features using the data gathered during this extended campaign. Simultaneous imaging in four bands (g, r, i, and zs) reveals a homogeneous composition and a reddish hue, resembling Solar system comets, and as well a diffuse profile exhibiting familiar cometary traits. We discern a stationary trend fluctuating around a constant activity level throughout 2019 October and November. Subsequently, a reduction in activity is observed in December. Dust production and mass-loss calculations indicate approximately an average of 4 kg s−1 before perihelion, while after perihelion the net mass-loss is about 0.6 kg s−1. Our simulations indicate the most probable size of coma dust particles should be in the range 200–250 nm, and the terminal speed around 300 m s−1. The spectrum acquired with the 4.2-m William Herschel Telescope shows the presence of a strong CN line for which we find a gas production rate of 1.2 × 1024 s−1. We also detected NH2 and OI bands. The ratio between NH2 and CN productions is log (NH2/CN) = −0.2. Overall, this observing campaign provides a new understanding of 2I/Borisov’s unique characteristics and activity patterns.
The relationship between emotion regulation and pain catastrophizing in patients with chronic pain
Authors: Yuan Yan , Schreiber Kristin , Flowers K Mikayla , Edwards Robert , Azizoddin Desiree , Ashcraft LauraEllen , Newhill Christina E , Hruschak Valerie
Published: February 2024

Objectives

Pain catastrophizing (PC) is a cognitive/emotional response to and in anticipation of pain that can be maladaptive, further exacerbating pain and difficulty in emotion regulation (ER). There is a lack of research on the interplay between PC and ER and its impact on pain. Our aim was to investigate whether ER exacerbated the pain experience through PC.

Methods

Adults with chronic non-cancer pain of >3 months’ duration (n = 150) who were taking opioid medication were recruited from a large medical center in Pennsylvania. A battery of questionnaires was conducted to gather data on demographics, substance use, mental health histories, and health and pain outcomes. Measures used included the 18-Item Difficulties in Emotion Regulation Scale, the Pain Catastrophizing Scale, the Brief Pain Inventory–Short Form, and the Hospital Anxiety and Depression Scale. A structural equation model with latent variables was conducted to examine our aim.

Results

Both pain interference and severity were significantly positively associated with several psychosocial variables, such as anxiety, depression, ER constructs, PC, and distress intolerance. The associations between subscales and pain interference were larger than the associations between subscales and pain severity. PC fully mediated the paths from ER to pain experiences.

Discussion

Our results highlight the importance of several cognitive and emotional constructs: nonacceptance of negative emotions, lack of emotional awareness, magnification of the pain experience, and a sense of helplessness. Furthermore, by showing the indirect effects of PC in affecting ER and pain, we posit that ER, mediated by PC, might serve a critical role in influencing the pain experience in patients with chronic pain.
Body composition, pulmonary function tests, exercise capacity, and quality of life in chronic obstructive pulmonary disease patients with obesity
Authors: Muršić Davorka , Glunčić Tajana Jalušić , Ostojić Jelena , Škrinjarić-Cincar Sanda , Kardum Ljiljana Bulat , Dokoza Martina , Lazarušić Nataša Karamarković , Bešić Erim , Samaržija Miroslav , Dugac Andrea Vukić
Published: February 2024

Purpose of the study

Larger proportions of chronic obstructive pulmonary disease (COPD) patients are currently overweight or with obesity than underweight, and the combination of COPD and obesity is increasing. The purpose of this study was to investigate differences in the body composition, pulmonary function tests, exercise capacity, and health-related quality of life among normal weight, overweight, and obese patients with COPD.

Study design

A total of 514 patients with COPD were included in the study. According to the World Health Organization criteria for body mass index, the patients were classified as normal weight, overweight, and obese. Evaluations included fat-free mass, fat-free mass index, phase angle, pulmonary function tests, and 6-minute walk test. Dyspnea was assessed using the modified Medical Research Council dyspnea scale, and the health-related quality of life was evaluated using COPD Assessment Test and St. George’s Respiratory Questionnaire. Values were compared among the three groups.

Results

There were 315 male and 199 female patients, with a mean age of 66.7 ± 8.4 years. Fat-free mass, fat-free mass index, and phase angle values were significantly higher in COPD patients with obesity than in other patients (P < .001, P < .001, P < .001). Forced expiratory volume in 1 s, forced expiratory volume in 1 s/forced vital capacity, and diffusing capacity of lung for carbon monoxide value in pulmonary function tests were significantly higher in COPD patients with obesity than in other patients (P = .046, P < .001, P < .001), while the forced vital capacity values were similar in all groups. Exercise capacity (6-min walk test distance), dyspnea symptoms (modified Medical Research Council scale), and health-related quality of life (COPD Assessment Test and St. George’s Respiratory Questionnaire) did not differ significantly between groups.

Conclusions

According to our study, obesity has no negative effect on pulmonary function tests, dyspnea perception, exercise capacity, and health-related quality of life.
New Classification Method for Equivalence Classes on S1/Z2 and T2/Z3 Orbifolds
Authors: Takeuchi Kota , Inagaki Tomohiro
Published: February 2024

In 5D and 6D U(N) and SU(N) gauge theories compactified on S1/Z2 and T2/Z3 orbifolds, we propose a new method to classify the equivalence classes (ECs) of boundary conditions (BCs) without depending on the structure of gauge transformations. Some of the BCs are connected through gauge transformations and constitute ECs, each of which contains physically equivalent BCs. Previous methods for classifying ECs have been used for specific gauge transformations. In this paper, we show that a geometric property of orbifolds significantly narrows down the possibilities of connecting BCs and completes the classification of ECs.
Can Human Capital Explain Income-Based Disparities in Financial Services?
Authors: Huang Ruidi , Linck James S , Mayer Erik J , Parsons Christopher A
Published: February 2024

Research shows that access to high-quality financial services varies with local income and wealth. We study how financial firms’ internal allocation of human capital contributes to these disparities. Using a near-comprehensive panel of over 350,000 U.S. mortgage loan officers, we document large and persistent differences in productivity and performance. We find that firms’ hiring and promotion practices allocate workers with less experience or poor track records to branches serving low-income customers. Further, the consequences of poor performance differ by location: low sales, bad loans, and misconduct are more tolerated in low-income branches, exacerbating income-based disparities in financial services.
Geriatric Pain Protocol: Impact of Multimodal Pain Care for Elderly Orthopedic Trauma Patients
Authors: Andujo Paulina , Yue Kelsey , McKelvey Karma , Dornan Grant J. , Breda Kathleen
Published: February 2024

Hip fractures are costly, and associated complications are the leading cause of injury-related deaths in persons 65 years or older. Uncontrolled pain leads to increased hospital length of stay (LOS), delayed physical therapy, and long-term functional impairment. The Geriatric Pain Protocol (GPP) is Cedars-Sinai's multimodal pain management solution, addressing the needs of older adult inpatients who have suffered fractures. Study participants included hip fracture patients admitted between February 1, 2019, and March 5, 2021. Inclusion criteria were patients 65 years or older with a hip fracture sustained from a ground-level fall and surgical candidate. Participants were divided into 2 categories: Geriatric Fracture Program (GFP) and non-GFP, with physician participation in the GFP being the differentiating factor. End points included postoperative pain, postoperative opioid utilization, LOS, complications, and 30-day readmission rates. The GPP decreased morphine milligram equivalent (MME) daily totals on days 1 and 2 and improved pain management compared with non-GPP patients. MMEs were lower in the GPP group than in the non-GPP group for both postoperative day 1 (POD1) (P = 0.007) and POD2 (P = 0.043); the Numerical Rating Scale (NRS) pain score on POD1 was lower in the GPP group (vs non-GPP, P = 0.013). There were no group differences in NRS POD2 pain or complications (all Ps > 0.1). The study sample (N = 453) had no significant difference between sex and LOS (all Ps > 0.3). Although not statistically significant, the 30-day readmission rate trended lower in patients treated in accordance with the GPP. Use of the GPP reduced pain levels and MME totals.
Overlooked Pain Assessment Records in Patients with Pressure Injuries During the COVID-19 Pandemic: A Retrospective Data Analysis
Authors: Sengul Tuba , Erden Sevilay , Karadağ Ayişe , Yilmaz Dilek , Gokduman Tugba
Published: February 2024

BACKGROUND

During the COVID-19 pandemic, healthcare professionals focused on identifying the cause of hemodynamic instability in patients and may have neglected to assess pressure injury (PI)-related pain. Although pain is an early indicator of PI development, there has been no systematic evaluation of PI-related pain in patients.

OBJECTIVE

To review nurses’ records of PI-related pain in patients who developed PIs during the COVID-19 pandemic.

METHODS

This retrospective, descriptive study included data from 510 patients at one hospital. Collected data included patient demographics (age, sex, diagnosis, and comorbidities), PI classification, and assessment of PI-related pain. Assessment data regarding PI-related pain included the characteristics of the pain, the type of analgesia (pharmacologic/nonpharmacologic) administered before and after PI management (debridement, dressing change, etc), the route of administration, and the frequency of pain assessment before and after analgesia.

RESULTS

The mean age of the patients (60.4% men) was 28.96 (SD, 5.82) years, and the mean length of hospital stay was 26.15 (SD, 16.1) days. Overall, 43.1% of the patients were treated in the ICU, 68.0% were conscious, and 18.6% tested positive for COVID-19. Deep-tissue injuries occurred in 57.5% of patients, with 48.6% developing stage 2 PI. The sacral region was the most common area for PI development (44.8%). The mean duration of repositioning in patients with PI was 23.03 (SD, 5.4) hours. Only 0.40% of patients (n = 2) were evaluated for pain, and only one patient was assessed for pain before and after analgesia was administered.

CONCLUSIONS

The findings suggest a lack of comprehensive evaluation and records concerning PI-related pain in patients with COVID-19.