bluuebunny/crossref_metadata_2025 huggingface dataset:
Three-Dimensional Reconstruction Method for Machined Surface Topography Based on Gray Gradient Constraints
Authors: Shi Wei-Chao , Zheng Jian-Ming , Li Yan , Li Xu-Bo
Published: February 2019

In the modern engineering field, recovering the machined surface topography is important for studying mechanical product function and surface characteristics by using the shape from shading (SFS)-based reconstruction method. However, due to the limitations of many constraints and oversmoothing, the existing SFS-based reconstruction methods are not suitable for machined surface topography. This paper presents a new three-dimensional (3D) reconstruction method of machined surface topography. By combining the basic principle of SFS and the analytic method, the analytic model of a surface gradient is established using the gray gradient as a constraint condition. By efficiently solving the effect of quantization errors and ambiguity of the gray scale on reconstruction accuracy using a wavelet denoising algorithm and image processing technology, the reconstruction algorithm is implemented for machined surface topography. Experimental results on synthetic images and machined surface topography images show that the proposed algorithm can accurately and efficiently recover the 3D shape of machined surface topography.
Potential of Workshop Measurement Positioning System to Measure Oscillation Frequencies of Rigid Structures
Authors: Xiong Chunbao , Bai Hongzhi , Lin Jiarui
Published: February 2019

A workshop measuring and positioning system (wMPS) is a large three-dimensional (3D) coordinate-measurement system based on optoelectronic scanning. It is capable of large-range coverage, high measurement accuracy and frequency, and multi-task synchronous measurement. Existing geodetic instruments cannot measure the intrinsic parameters of strong rigid structures. Thus, this study conducted experiments to explore the feasibility of the wMPS to measure the intrinsic parameters of rigid structures. A test bed was established using a reverse-engineering method to simulate the oscillation frequency of the structure. Displacement data, which changed with the time series through the fast Fourier transformation method, were analyzed to determine the feasibility and range of the wMPS in measuring intrinsic parameters of the structure. The experimental results demonstrated that the wMPS can measure the vibrational frequency up to 9 Hz with a 3-mm amplitude and up to 4 Hz with a 30-mm amplitude.
Utility of FIB4-T as a Prognostic Factor for Hepatocellular Carcinoma
Authors: Kariyama Kazuya , Nouso Kazuhiro , Toyoda Hidenori , Tada Toshifumi , Hiraoka Atsushi , Tsuji Kunihiko , Itobayashi Ei , Ishikawa Toru , Wakuta Akiko , Oonishi Ayano , Kumada Takashi , Kudo Masatoshi
Published: February 2019

Background: Most integrated scores for predicting the prognosis of patients with hepatocellular carcinoma (HCC) comprise tumor progression factors and liver function variables. The FIB4 index is an indicator of hepatic fibrosis calculated on the basis of age, aspartate aminotransferase (AST) levels, alanine aminotransferase (ALT) levels, and platelet count, but it does not include variables directly related to liver function. We propose a new staging system, referred to as “FIB4-T,” comprising the FIB4 index as well as tumor progression factors, and examine its usefulness. Method: Subjects included 3800 cases of HCC registered in multiple research centers. We defined grades 1, 2, and 3 as a Fibrosis-4 (FIB4) index of <3.25, 3.26–6.70, and >6.70 as FIB4, respectively, and calculated the FIB4-T in the same manner in which the JIS (Japan Integrated Staging Score) scores and albumin-bilirubin tumor node metastasis (ALBI-T) were calculated. We compared the prognostic prediction ability of FIB4-T with that of the JIS score and ALBI-T. Results: Mean observation period was 37 months. The 5-year survival rates (%) of JIS score (0/1/2/3/4/5), ALBI-T (0/1/2/3/4/5) and FIB4-T (0/1/2/3/4/5) were 74/60/36/16/0, 82/66/45/22/5/0 and 88/75/65/58/32/10, respectively. Comparisons of the Akaike information criteria among JIS scores, ALBI-T, and FIB4-T indicated that stratification using the FIB4-T system was comparable to those using ALBI-T and JIS score. The risk of mortality significantly increased (1.3–2.8 times/step) with an increase in FIB4-T, and clear stratification was possible regardless of the treatment. Conclusions: FIB4-T is useful in predicting the prognosis of patients with HCC from a new perspective.
Zoledronic Acid Modulation of TRPV1 Channel Currents in Osteoblast Cell Line and Native Rat and Mouse Bone Marrow-Derived Osteoblasts: Cell Proliferation and Mineralization Effect
Authors: Scala Rosa , Maqoud Fatima , Angelelli Mariacristina , Latorre Ramon , Perrone Maria Grazia , Scilimati Antonio , Tricarico Domenico
Published: February 2019

Bisphosphonates (BPs) reduce bone pain and fractures by balancing the osteoblast/osteoclast ratio. The behavior of ion channels in the presence of BPs is not known. To investigate this, the effect of zoledronic acid BP (ZOL) (3 × 10−8 to 5 × 10−4 M) treatment, on ion channels, cell proliferation, and mineralization, has been investigated on preosteoclast-like cells, RAW264.7, preosteoblast-like cells MC3T3-E1, and rat/mouse native bone marrow-derived osteoblasts. In whole-cell patch clamp on cell line- and bone marrow-derived osteoblasts, ZOL potentiated outward currents. On RAW264.7, ZOL (10−4 M)-evoked current was reduced by the Kv channel blocker tetraethylammonium hydrochloride (TEA), but not by the selective TRPV1-channel antagonist capsazepine. On MC3T3-E1 cells and bone marrow-derived osteoblasts, ZOL-evoked current (5 × 10−8 to 10−4 M) was reduced by capsazepine, whereas the selective TRPV1-channel agonist capsaicin potentiated the control current. In the cell proliferation assay, 72 h incubation of RAW264.7 and MC3T3-E1 cells with ZOL reduced proliferation, with IC50 values of 2.62 × 10−7 M and 2.02 × 10−5 M, respectively. Mineralization of MC3T3-E1 cells and bone marrow-derived osteoblasts was observed in the presence of capsaicin and ZOL (5 × 10−8–10−7 M); ZOL effects were antagonized by capsazepine. In summary, the ZOL-induced activation of TRPV1 channel mediates the mineralization of osteoblasts and counterbalances the antiproliferative effects, increasing the IC50. This mechanism is not operative in osteoclasts lacking the TRPV1 channel.
ORAI1 and ORAI3 in Breast Cancer Molecular Subtypes and the Identification of ORAI3 as a Hypoxia Sensitive Gene and a Regulator of Hypoxia Responses
Authors: Azimi Iman , Milevskiy Michael , Chalmers Silke , Yapa Kunsala , Robitaille Mélanie , Henry Christopher , Baillie Gregory , Thompson Erik , Roberts-Thomson Sarah , Monteith Gregory
Published: February 2019

The remodeling of specific calcium-permeable ion channels is a feature of some breast cancer subtypes. ORAI1 is a protein that forms a calcium-permeable ion channel responsible for store-operated calcium entry (SOCE) in a variety of cell types. ORAI3, a related isoform, is not a regulator of SOCE in most cell types. However, ORAI3 does control SOCE in many estrogen receptor-positive breast cancer cell lines, where it also controls proliferation. ORAI1 is a well-characterized regulator of the proliferation and migration of many basal breast cancer cells; however, the role of ORAI3 in these types of breast cancer cells remains unclear. Here, we sought to define ORAI1 and ORAI3 expression in breast cancer cell lines of different molecular subtypes and assess the potential role and regulation of ORAI3 in basal breast cancer cells. Our study demonstrates that elevated ORAI1 is a feature of basal-like breast cancers, while elevated ORAI3 is a feature of luminal breast cancers. Intriguingly, we found that ORAI3 is over-expressed in the mesenchymal subtype of triple-negative breast cancer. Given this, we assessed ORAI3 levels in the presence of two inducers of the mesenchymal phenotype, hypoxia and epidermal growth factor (EGF). Hypoxia induced ORAI3 levels in basal breast cancer cell lines through a pathway involving hypoxia-inducible factor-1 alpha (HIF1α. The silencing of ORAI3 attenuated hypoxia-associated phosphorylation of the EGF receptor (EGFR) and the expression of genes associated with cell migration and inflammatory/immune responses in the MDA-MB-468 model of basal breast cancer. Although elevated ORAI3 levels were not associated with survival; basal, estrogen receptor-negative and triple-negative breast cancers with high ORAI3 and low ORAI1 levels were associated with poorer clinical outcomes. This study defines ORAI3 as a potential fine-tuner for processes relevant to the progression of basal breast cancers.
An Early Decrease in Release of Aquaporin-2 in Urinary Extracellular Vesicles After Cisplatin Treatment in Rats
Authors: Sonoda Hiroko , Oshikawa-Hori Sayaka , Ikeda Masahiro
Published: February 2019

Aquaporin-1 (AQP1) and AQP2 are important proteins involved in the regulation of renal water handling. Both AQPs have been found in urinary extracellular vesicles (uEVs) (uEV-AQP1 and -AQP2). Cisplatin, an antineoplastic agent, is known to down-regulate renal AQP1 and AQP2. However, the effect of cisplatin on the release of uEV-AQP1 and -AQP2 is largely unknown. In this study, we examined whether treatment of rats with cisplatin affected the release of uEV-AQP1 and -AQP2. Blood tests indicated that renal function was little altered at 24 h after cisplatin treatment but thereafter decreased dramatically at all of the other time points examined. Release of uEV-AQP1 was slightly increased at 24 h and decreased at 168 h. On the other hand, release of uEV-AQP2 was decreased dramatically at 24 h, and the decrease was maintained during the experimental period. These data suggest that uEV-AQP2 can be used to detect early renal impairment due to cisplatin. Furthermore, a combination of uEV-AQP2 and -AQP1 may be useful for estimation of cisplatin-induced renal injury in a stage-dependent manner.
Epithelial-To-Mesenchymal Transition Markers and CD44 Isoforms Are Differently Expressed in 2D and 3D Cell Cultures of Prostate Cancer Cells
Authors: Fontana Fabrizio , Raimondi Michela , Marzagalli Monica , Sommariva Michele , Limonta Patrizia , Gagliano Nicoletta
Published: February 2019

Three-dimensional (3D) cell cultures allow the mimic of functions of living tissues and provide key information encoded in tissue architecture. Considered the pivotal role of epithelial-to-mesenchymal transition (EMT) in carcinoma progression, including prostate cancer (PCa), we aimed at investigating the effect of the 3D arrangement on the expression of some key markers of EMT in cultured human prostate cancer (PCa) cells, to better understand PCa cell behavior. PC3 and DU145 PCa cells were cultured in RPMI cell culture medium either in 2D-monolayers or in 3D-spheroids. The main EMT markers E-cadherin, N-cadherin, α-smooth muscle actin (αSMA), vimentin, Snail, Slug, Twist and Zeb1 were evaluated by confocal microscopy, real-time PCR and Western blot. Confocal microscopy revealed that E-cadherin was similarly expressed at the cell boundaries on the plasma membrane of PCa cells grown in 2D-monolayers, as well as in 3D-spheroids, but resulted up-regulated in 3D-spheroids, compared to 2D-monolayers, at the mRNA and protein level. Moreover, markers of the mesenchymal phenotype were expressed at very low levels in 3D-spheroids, suggesting important differences in the phenotype of PCa cells grown in 3D-spheroids or in 2D-monolayers. Considered as a whole, our findings contribute to a clarification of the role of EMT in PCa and confirm that a 3D cell culture model could provide deeper insight into the understanding of the biology of PCa.
Focus on Cdc42 in Breast Cancer: New Insights, Target Therapy Development and Non-Coding RNAs
Authors: Zhang Yu , Li Jun , Lai Xing-Ning , Jiao Xue-Qiao , Xiong Jun-Ping , Xiong Li-Xia
Published: February 2019

Breast cancer is the most common malignant tumors in females. Although the conventional treatment has demonstrated a certain effect, some limitations still exist. The Rho guanosine triphosphatase (GTPase) Cdc42 (Cell division control protein 42 homolog) is often upregulated by some cell surface receptors and oncogenes in breast cancer. Cdc42 switches from inactive guanosine diphosphate (GDP)-bound to active GTP-bound though guanine-nucleotide-exchange factors (GEFs), results in activation of signaling cascades that regulate various cellular processes such as cytoskeletal changes, proliferation and polarity establishment. Targeting Cdc42 also provides a strategy for precise breast cancer therapy. In addition, Cdc42 is a potential target for several types of non-coding RNAs including microRNAs and lncRNAs. These non-coding RNAs is extensively involved in Cdc42-induced tumor processes, while many of them are aberrantly expressed. Here, we focus on the role of Cdc42 in cell morphogenesis, proliferation, motility, angiogenesis and survival, introduce the Cdc42-targeted non-coding RNAs, as well as present current development of effective Cdc42-targeted inhibitors in breast cancer.
Purification of Functional Human TRP Channels Recombinantly Produced in Yeast
Authors: Zhang Liying , Wang Kaituo , Klaerke Dan Arne , Calloe Kirstine , Lowrey Lillian , Pedersen Per Amstrup , Gourdon Pontus , Gotfryd Kamil
Published: February 2019

(1) Background: Human transient receptor potential (TRP) channels constitute a large family of ion-conducting membrane proteins that allow the sensation of environmental cues. As the dysfunction of TRP channels contributes to the pathogenesis of many widespread diseases, including cardiac disorders, these proteins also represent important pharmacological targets. TRP channels are typically produced using expensive and laborious mammalian or insect cell-based systems. (2) Methods: We demonstrate an alternative platform exploiting the yeast Saccharomyces cerevisiae capable of delivering high yields of functional human TRP channels. We produce 11 full-length human TRP members originating from four different subfamilies, purify a selected subset of these to a high homogeneity and confirm retained functionality using TRPM8 as a model target. (3) Results: Our findings demonstrate the potential of the described production system for future functional, structural and pharmacological studies of human TRP channels.
Alterations in Platelet Alpha-Granule Secretion and Adhesion on Collagen under Flow in Mice Lacking the Atypical Rho GTPase RhoBTB3
Authors: Berger Martin , Riley David , Lutz Julia , Khalil Jawad , Aburima Ahmed , Naseem Khalid , Rivero Francisco
Published: February 2019

Typical Rho GTPases, such as Rac1, Cdc42, and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB subfamily are characterized by divergent domain architecture. One family member, RhoBTB3, is expressed in platelets, but its function is unclear. In the present study we examined the role of RhoBTB3 in platelet function using a knockout mouse model. We found the platelet count, size, numbers of both alpha and dense granules, and surface receptor profile in these mice were comparable to wild-type mice. Deletion of Rhobtb3 had no effect on aggregation and dense granule secretion in response to a range of agonists including thrombin, collagen, and adenosine diphosphate (ADP). By contrast, alpha-granule secretion increased in mice lacking RhoBTB3 in response to thrombin, collagen related peptide (CRP) and U46619/ADP. Integrin activation and spreading on fibrinogen and collagen under static conditions were also unimpaired; however, we observed reduced platelet accrual on collagen under flow conditions. These defects did not translate into alterations in tail bleeding time. We conclude that genetic deletion of Rhobtb3 leads to subtle alterations in alpha-granule secretion and adhesion to collagen without significant effects on hemostasis in vivo.
High Dynamic Range Image Deghosting Using Spectral Angle Mapper
Authors: Khan Muhammad Murtaza
Published: February 2019

The generation of high dynamic range (HDR) images in the presence of moving objects results in the appearance of blurred objects. These blurred objects are called ghosts. Over the past decade, numerous deghosting techniques have been proposed for removing blurred objects from HDR images. These methods may try to identify moving objects and maximize dynamic range locally or may focus on removing moving objects and displaying static objects while enhancing the dynamic range. The resultant image may suffer from broken/incomplete objects or noise, depending upon the type of methodology selected. Generally, deghosting methods are computationally intensive; however, a simple deghosting method may provide sufficiently acceptable results while being computationally inexpensive. Inspired by this idea, a simple deghosting method based on the spectral angle mapper (SAM) measure is proposed. The advantage of using SAM is that it is intensity independent and focuses only on identifying the spectral—i.e., color—similarity between two images. The proposed method focuses on removing moving objects while enhancing the dynamic range of static objects. The subjective and objective results demonstrate the effectiveness of the proposed method.
The Variational Reduction for Low-Dimensional Fermi Gases and Bose–Fermi Mixtures: A Brief Review
Authors: Díaz Pablo , Laroze David , Malomed Boris A.
Published: February 2019

We present a summary of some recent theoretical results for matter-wave patterns in Fermi and Bose–Fermi degenerate gases, obtained in the framework of the quasi-mean-field approximation. We perform a dimensional reduction from the three-dimensional (3D) equations of motion to 2D and 1D effective equations. In both cases, comparison of the low-dimensional reductions to the full model is performed, showing very good agreement for ground-state solutions. Some complex dynamical regimes are reported too for the corresponding 1D systems.
Experiences of Being a Parent to a Child with Amelogenesis Imperfecta
Authors: Pousette Lundgren Gunilla , Hasselblad Tove , Johansson Anna Stigsdotter , Johansson Anna , Dahllöf Göran
Published: February 2019

Amelogenesis imperfecta (AI) is a hereditary developmental disorder affecting the enamel of teeth. Affected patients present with tooth hypersensitivity, rapid tooth wear, or fractures of enamel as well as alterations in color and shape, all of which compromise esthetic appearance and masticatory function. Chronic conditions in childhood severely impact the whole family, affecting normal family routines and/or increasing the family’s financial burden. The aim of this study was to explore experiences and the impact on daily life of being a parent to a child with severe forms of amelogenesis imperfecta. Parents of children and adolescents with AI participated in an interview with a psychologist. The transcribed interviews were analyzed using thematic analysis. The parents talked about several concerns about having a child with AI. Four main themes emerged from the interviews: Feelings associated with passing on a hereditary disorder, knowledge decreases stress, unfamiliarity with the diagnosis, and psychosocial stress. In these main categories we identified several subthemes. Feelings associated with passing on a hereditary disorder included the subtheme of guilt/shame; knowledge decreases stress included knowledge about diagnosis in the family and support from dental health care professionals; Unfamiliarity with diagnosis included missed diagnosis, fear of not getting correct treatment, and insufficient pain control; finally, the subtheme Psychosocial stress included fear of child being bullied and emergency dental visits. The findings show that parents of children with severe amelogenesis imperfecta report similar experiences as do parents of children with other chronic and rare diseases.
A Hybrid Communication Scheme for Efficient and Low-Cost Deployment of Future Flying Ad-Hoc Network (FANET)
Authors: Khan Muhammad , Qureshi Ijaz , Khanzada Fahimullah
Published: February 2019

In recent years, FANET-related research and development has doubled, due to the increased demands of unmanned aerial vehicles (UAVs) in both military and civilian operations. Equipped with more capabilities and unique characteristics, FANET is able to play a vital role in mission-critical applications. However, these distinctive features enforce a series of guidelines to be considered for its efficient deployment. Particularly, the use of FANET for on-time data communication services presents demanding challenges in terms of energy efficiency and quality of service (QoS). Proper use of communication architecture and wireless technology will assist to solve these challenges. Therefore, in this paper, we review different communication architectures, including the existing wireless technologies, in order to provide seamless wireless connectivity. Based on the discussions, we conclude that a multi-layer UAV ad-hoc network is the most suitable architecture for networking a group of heterogeneous UAVs, while Bluetooth 5 (802.15.1) is the most favored option because of its low-cost, low power consumption, and longer transmission range for FANET. However, 802.15.1 has the limitation of a lower data rate as compared to Wi-Fi (802.11). Therefore, we propose a hybrid wireless communication scheme so as to utilize the features of the high data transmission rate of 802.11 and the low-power consumption of 802.15.1. The proposed scheme significantly reduces communication cost and improves the network performance in terms of throughput and delay. Further, simulation results using the Optimized Network Engineering Tool (OPNET) further support the effectiveness of our proposed scheme.
A Novel SWB Antenna with Triple Band-Notches Based on Elliptical Slot and Rectangular Split Ring Resonators
Authors: Zhang Xiaobo , Ur Rahman Saeed , Cao Qunsheng , Gil Ignacio , khan Muhammad Irshad
Published: February 2019

In this paper, a wideband antenna was designed for super-wideband (SWB) applications. The proposed antenna was fed with a rectangular tapered microstrip feed line, which operated over a SWB frequency range (1.42 GHz to 50 GHz). The antenna was implemented at a compact size with electrical dimensions of 0.16 λ × 0.27 λ × 0.0047 λ mm3, where λ was with respect to the lowest resonance frequency. The proposed antenna prototype was fabricated on a F4B substrate, which had a permittivity of 2.65 and 1 mm thickness. The SWB antenna exhibited an impedance bandwidth of 189% and a bandwidth ratio of 35.2:1. Additionally, the proposed antenna design exhibited three band notch characteristics that were necessary to eradicate interference from WLAN, WiMAX, and X bands in the SWB range. One notch was achieved by etching an elliptical split ring resonator (ESRR) in the radiator and the other two notches were achieved by placing rectangular split ring resonators close to the signal line. The first notch was tuned by incorporating a varactor diode into the ESRR. The prototype was experimentally validated with, with notch and without notch characteristics for SWB applications. The experimental results showed good agreement with simulated results.
3D Printing Using a 60 GHz Millimeter Wave Segmented Parabolic Reflective Curved Antenna
Authors: Cai Benxiao , Sun Lingling , Lei Yuchao
Published: February 2019

This paper proposes a segmented parabolic curved antenna, which can be used in the base station of a 60 GHz millimeter wave communication system, with an oblique Yagi antenna as a feed. By analyzing the reflection and multi-path interference cancellation phenomenon when the main lobe of the Yagi antenna is reflected, the problem of main lobe splitting is solved. 3D printing technology relying on PLA (polylactic acid) granule raw materials was used to make the coaxial connector bracket and segmented parabolic surface. The reflective surface was vacuum coated (via aluminum evaporation) with low-loss aluminum. The manufacturing method is environmentally friendly and the structure was printed with 0.1 mm accuracy based on large-scale commercial applications at a low cost. The experimental results show that the reflector antenna proposed in this paper achieves a high gain of nearly 20 dBi in 57–64 GHz frequency band and ensures that the main lobe does not split.
Numerical Comparison of Thermohydraulic Performance and Fluid-Induced Vibrations for STHXs with Segmental, Helical, and Novel Clamping Antivibration Baffles
Authors: Naqvi S.M.A. , Wang Qiuwang
Published: February 2019

The most extensively used heat exchanger in numerous research fields and industrial processes is the shell and tube heat exchanger. The selection of the baffle plays a vital role to regulate and increase the thermohydraulic performance and also to decrease fluid-induced vibrations due to shell side flow. 3-D computational fluid dynamics (CFD) and fluid-structure interaction (FSI) have been done to analyze the pressure drop, heat transfer coefficient, vortex shedding, and tube deformation due to induced vibrations among the recently developed clamping antivibration baffles with square twisted tubes, helical baffles with cylindrical tubes, and conventional segmental baffles with cylindrical tubes at different shell side flow rates by using commercial software ANSYS. Complete heat exchangers are modeled for numerical comparison; the thermohydraulic performance of the numerical model shows the suitable agreement by validating it with already published results and Esso method for single segmental baffles. It is then used to compare the performance of the same heat exchangers with CBSTT and HBCT. Thermohydraulic performance of CBSTT-STHX is better than SGCT-STHX. The heat transfer coefficient of heat exchangers with tube-to-baffle-hole clearance is higher and there is a reduction in the pressure drop compared to the results of STHXs without tube-to-baffle-hole clearance. The deformation in the tubes and vortex-induced vibrations are minimum in STHX with CBSTT than in STHXs with HBCT and SGCT.
Thermal Non-Equilibrium Heat Transfer Modeling of Hybrid Nanofluids in a Structure Composed of the Layers of Solid and Porous Media and Free Nanofluids
Authors: Chamkha Ali J. , Sazegar Sina , Jamesahar Esmael , Ghalambaz Mohammad
Published: February 2019

The free convection heat transfer of hybrid nanofluids in a cavity space composed of a clear flow, porous medium and a solid part is addressed. The cavity is heated from the bottom and cooled from the top. The side walls are well insulated. The upper part of the cavity is a clear space with no porous or solid materials and is filled with hybrid nanofluid. The bottom part is divided into two parts of a porous space saturated with the hybrid nanofluid and a solid thermal conductive block. There are conjugate heat transfer mechanisms between the solid block and the porous medium filled with the hybrid nanofluid as well as the hybrid nanofluid in the clear space. For the porous medium model, the local thermal non-equilibrium effects are considered. The hybrid nanofluids contain copper (20 nm) and alumina nanoparticles (40 nm) hybrid nanoparticles. The governing equations for the flow and heat transfer of the hybrid nanofluid in the clear space and the porous medium are introduced. Considering the conjugate heat transfer between the solid block and the hybrid nanofluid fluid in the pores and the porous matrix, appropriate boundary conditions for heat channeling are utilized. The governing equations are transformed into non-dimensional form to generalize the model. The finite element method is employed to solve the equations. The grid check and validation procedure are performed. Subsequently streamlines, isotherms, and Nusselt number are studied as important aspects of flow and heat transfer in the cavity. The increase in the portion of the clear flow part in the cavity enhances heat transfer due to better hybrid nanofluid circulation.
Numerical Study on Thermal Hydraulic Performance of Supercritical LNG in Zigzag-Type Channel PCHEs
Authors: Zhao Zhongchao , Zhou Yimeng , Ma Xiaolong , Chen Xudong , Li Shilin , Yang Shan
Published: February 2019

In this paper, we study a promising plate-type heat exchanger, the printed circuit heat exchanger (PCHE), which has high compactness and is suitable for high-pressure conditions as a vaporizer during vaporization. The thermal hydraulic performance of supercritical produce liquefied natural gas (LNG) in the zigzag channel of PCHE is numerically investigated using the SST κ-ω turbulence model. The thermo-physical properties of supercritical LNG from 6.5 MPa to 10MPa were calculated using piecewise-polynomial approximations of the temperature. The effect of the channel bend angle, mass flux and inlet pressure on local convection heat transfer coefficient, and pressure drop are discussed. The heat transfer and pressure loss performance are evaluated using the Nusselt and Euler numbers. Nu/Eu is proposed to evaluate the comprehensive heat transfer performance of PCHE by considering the heat transfer and pressure drop characteristics to find better bend angle and operating conditions. The supercritical LNG has a better heat transfer performance when bend angle is less than 15° with the mass flux ranging from 207.2 kg/(m2·s) to 621.6 kg/(m2·s), which improves at bend angle of 10° and lower compared to 15° at mass flux above 414.4 kg/(m2·s). The heat transfer performance is better at larger mass flux and lower operating pressures.
Timber and Branch Volume Prediction: Effects of Stand and Site Characteristics on Dendromass and Timber-To-Branch Volume Ratio of Norway Spruce in Managed Forests
Authors: Štícha Václav , Sharma Ram P. , Vacek Zdeněk , Vacek Stanislav , Nuhlíček Ondřej
Published: February 2019

The objective of this study was to develop the models that predict both timber and branch volumes of Norway spruce (Picea abies/L./Karst.), the most abundant tree species in Europe, and determine the relationships among timber and branch volumes and various site and stand characteristics. The data used in this study come from 76 sample plots in the different stands and site conditions across Norway spruce forests in the Czech Republic. Timber volume was determined by Huber’s formula and branch volume (logging residue) was determined by drying and weighing of 10 samples from the 10-chipped trees on each sample plot, meaning that a total of 760 samples were analyzed. The results showed that timber volume was significantly positively correlated with branch volume, mean diameter at breast height (mean DBH) per sample plot, mean height per sample plot, slope of sample plot, and stand age, but negatively correlated with stand stocking. The branch volume was more significantly affected by stand stocking than timber volume. The timber-to-branch volume ratio (TBR) reached the mean value of 3.7 (±0.14 SE) and significantly increased with increasing elevation. The trees on the nutrient-rich sites were characterized by higher branch volume, while TBR reached higher values on the acid sites. Site quality class had a significant effect only on the branch volume production. Compared to the timber volume (root mean square error, RMSE = 3.6176; adjusted coefficient of determination, R2adj = 0.7310), the branch volume was relatively poorly described by the model (RMSE = 1.928; R2adj = 0.2517). The volume prediction models show that timber volumes increase with increasing slope and branch volume increases with decreasing site quality class. For effective forest management practice, the highest branch volume in favor of timber production is characterized for lowland forests with stand stocking ≤60% (TBR 1.5), while the highest share of timber volume (TBR 9.5) can be reached in the mountains with a full stand stocking.
Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume
Authors: Zheng Tangchun , Zhuo Xiaokang , Li Lulu , Wang Jia , Cheng Tangren , Zhang Qixiang
Published: February 2019

Cyclins, a prominent class of cell division regulators, play an extremely important role in plant growth and development. D-type cyclins (CYCDs) are the rate-limiting components of the G1 phase. In plants, studies of CYCDs are mainly concerned with herbaceous plants, yet little information is available about these genes in perennial woody plants, especially ornamental plants. Here, twelve Prunus mume CYCD (PmCYCDs) genes are identified and characterized. The PmCYCDs were named on the basis of orthologues in Arabidopsis thaliana and Oryza sativa. Gene structure and conserved domains of each subgroup CYCDs was similar to that of their orthologues in A. thaliana and O. sativa. However, PmCYCDs exhibited different tissue-specific expression patterns in root, stem, leaf, bud, and fruit organs. The results of qRT-PCR showed that all PmCYCDs, except PmCYCD5;2 and PmCYCD7;1, were primarily highly expressed in leaf buds, shoots, and stems. In addition, the transcript levels of PmCYCD genes were analyzed in roots under different treatments, including exogenous applications of NAA, 6-BA, GA3, ABA, and sucrose. Interestingly, although PmCYCDs were induced by sucrose, the extent of gene induction among PmCYCD subgroups varied. The induction of PmCYCD1;2 by hormones depended on the presence of sucrose. PmCYCD3;1 was stimulated by NAA, and induction was strengthened when sugar and hormones were applied together. Taken together, our study demonstrates that PmCYCDs are functional in plant stem development and provides a basis for selecting members of the cyclin gene family as candidate genes for ornamental plant breeding.
Overstory–Understory Vegetation Cover and Soil Water Content Observations in Western Juniper Woodlands: A Paired Watershed Study in Central Oregon, USA
Authors: Ray Grace , Ochoa Carlos G. , Deboodt Tim , Mata-Gonzalez Ricardo
Published: February 2019

The effects of western juniper (Juniperus occidentalis) control on understory vegetation and soil water content were studied at the watershed-scale. Seasonal differences in topsoil (12 cm) water content, as affected by vegetation structure and soil texture, were evaluated in a 96-ha untreated watershed and in a 116-ha watershed where 90% juniper was removed in 2005. A watershed-scale characterization of vegetation canopy cover and soil texture was completed to determine some of the potential driving factors influencing topsoil water content fluctuations throughout dry and wet seasons for approximately one year (2014–2015). We found greater perennial grass, annual grass, and shrub cover in the treated watershed. Forb cover was no different between watersheds, and as expected, tree canopy cover was greater in the untreated watershed. Results also show that on average, topsoil water content was 1% to 3% greater in the treated watershed. The exception was during one of the wettest months (March) evaluated, when soil water content in the untreated watershed exceeded that of the treated by <2%. It was noted that soil water content levels that accumulated in areas near valley bottoms and streams were greater in the treated watershed than in the untreated toward the end of the study in late spring. This is consistent with results obtained from a more recent study where we documented an increase in subsurface flow residence time in the treated watershed. Overall, even though average soil water content differences between watersheds were not starkly different, the fact that more herbaceous vegetation and shrub cover were found in the treated watershed led us to conclude that the long-term effects of juniper removal on soil water content redistribution throughout the landscape may be beneficial towards restoring important ecohydrologic connections in these semiarid ecosystems of central Oregon.
Sustainable Development Goals and the Forest Sector—A Complex Relationship
Authors: Baumgartner Rupert J.
Published: February 2019

The United Nations adopted the Agenda 2030 with its core element, the 17 Sustainable Development Goals (SDGs), in September 2015. In order to achieve these goals within the coming years, intense efforts are required by all political and societal actors. Although the first definitions of sustainable development referred to the forest sector, the question remains: what contribution can forestry make to achieving the Sustainable Development Goals? Therefore, the direct positive and negative effects of forestry itself on sustainability are analyzed, and it is discussed how sustainable forest management could contribute to achieving other Sustainable Development Goals in addition to SDG 15. This analysis reveals that forestry plays a dual role, i.e., forestry can achieve positive sustainability effects but can also have negative impacts. It is thus recommended to use integrated assessment approaches to analyze whether a specific forest-related policy or strategy is contributing to sustainable development. Beside quantitative integrated assessments, the use of qualitative frameworks like the Framework for Strategic Sustainable Development is proposed. It is also suggested to operationalize the concept of second-order sustainability performance for the forest sector in future research.
Transcriptome Analysis of Male and Female Mature Gonads of Silver Sillago (Sillago sihama)
Authors: Tian Changxu , Li Zhiyuan , Dong Zhongdian , Huang Yang , Du Tao , Chen Huapu , Jiang Dongneng , Deng Siping , Zhang Yulei , Wanida Saetan , Shi Hongjuan , Wu Tianli , Zhu Chunhua , Li Guangli
Published: February 2019

Silver sillago (Sillago sihama) is an emerging commercial marine aquaculture species in China. To date, fundamental information on S. sihama, such as genomic information, is lacking, and no data are available on the gonad transcriptome of S. sihama. Here, the first gonadal transcriptomes of S. sihama have been constructed and genes potentially involved in gonadal development and reproduction identified. Illumina sequencing generated 60.18 million clean reads for the testis and 59.10 million for the ovary. All reads were assembled into 74,038 unigenes with a mean length of 1,004 bp and N50 value of 2,190 bp. Among all the predictable unigenes, a total of 34,104 unigenes (46%) were searched against multiple databases, including 33,244 unigenes annotated in the RefSeq Non- Redundant database at NCBI, and 28,924 in Swiss-Prot. By comparing the ovary and testis, 35,367 unigenes were identified as being differentially expressed between males and females, of which 29,127 were upregulated in the testis and 6,240 were upregulated in the ovary. Numerous differentially expressed genes (DEGs) known to be involved in gonadal development and gametogenesis were identified, including amh, dmrt1, gsdf, cyp19a1a, gnrhr, and zps. Using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses, the top 20 KEGG pathways with highest number of DEGs were found to be involved in regulating gonadal development and gametogenesis in S. sihama. Moreover, 22,666 simple sequence repeats (SSRs) were identified in 14,577 SSR-containing sequences. The findings provide a valuable dataset for future functional analyses of sex-associated genes and molecular marker assisted selection in S. sihama.
Comparison of the Effects of Hand Reflexology versus Acupressure on Anxiety and Vital Signs in Female Patients with Coronary Artery Diseases
Authors: Rahmani Vasokolaei Zohre , Rejeh Nahid , Heravi-Karimooi Majideh , Tadrisi Seyed Davood , Saatchi Kiarash , Poshtchaman Zahra , Sieloff Christina , Vaismoradi Mojtaba
Published: February 2019

Hospitalization in the cardiac care unit can increase anxiety in patients. This study aimed to compare hand reflexology versus acupressure on anxiety and vital signs in female patients with coronary artery diseases. This double-blinded randomized placebo-controlled trial with a pre- and post-intervention design was conducted on 135 female patients with coronary artery diseases. Female patients hospitalized in a cardiac care unit were randomly divided into three groups of hand reflexology, acupressure and placebo (n = 45 patients in each group) using blocking and a table of random numbers. Data was collected using the Spielberger anxiety inventory. Also, their vital signs were measured before, immediately after and half an hour after the intervention. Data analysis was performed using descriptive and analytical statistics. Before the intervention, there was no statistically significant difference in anxiety levels between the groups (p > 0.05). Also, the effects of hand reflexology and acupressure immediately and half an hour later on the reduction of anxiety and vital signs were equal (p < 0.05). Implementation of hand reflexology and acupressure can have positive effects on anxiety and vital signs in patients with coronary artery diseases. They can reduce patients’ anxiety with an equal effectiveness.
Some Challenges in Hydrologic Model Calibration for Large-Scale Studies: A Case Study of SWAT Model Application to Mississippi-Atchafalaya River Basin
Authors: Kannan Narayanan , Santhi Chinnasamy , White Michael J. , Mehan Sushant , Arnold Jeffrey G. , Gassman Philip W.
Published: February 2019

This study is a part of the Conservation Effects Assessment Project (CEAP) aimed to quantify the environmental and economic benefits of conservation practices implemented in the cultivated cropland throughout the United States. The Soil and Water Assessment Tool (SWAT) model under the Hydrologic United Modeling of the United States (HUMUS) framework was used in the study. An automated flow calibration procedure was developed and used to calibrate runoff for each 8-digit watershed (within 20% of calibration target) and the partitioning of runoff into surface and sub-surface flow components (within 10% of calibration target). Streamflow was validated at selected gauging stations along major rivers within the river basin with a target R2 of >0.6 and Nash and Sutcliffe Efficiency of >0.5. The study area covered the entire Mississippi and Atchafalaya River Basin (MARB). Based on the results obtained, our analysis pointed out multiple challenges to calibration such as: (1) availability of good quality data, (2) accounting for multiple reservoirs within a sub-watershed, (3) inadequate accounting of elevation and slopes in mountainous regions, (4) poor representation of carrying capacity of channels, (5) inadequate capturing of the irrigation return flows, (6) inadequate representation of vegetative cover, and (7) poor representation of water abstractions (both surface and groundwater). Additional outstanding challenges to large-scale hydrologic model calibration were the coarse spatial scale of soils, land cover, and topography.
Forecast and Analysis on Reducing China’s CO2 Emissions from Lime Industrial Process
Authors: Tong Qing , Zhou Sheng , Guo Yuefeng , Zhang Yang , Wei Xinyang
Published: February 2019

China greenhouse gas inventories show that CO2 emissions from the lime industrial process are large scales and closely related to the development of its downstream industries. Therefore, there is high importance to analyze and forecast on reducing China’s CO2 emissions from lime industrial process. The aims of this paper are to make up the research gaps in China and provide a quantitative reference for related authorities to formulate relevant policies. The prediction method in this paper is consistent with the published national inventory, which is an activity data based method to predict carbon dioxide emissions from the industrial process of four categories of lime products. Three future scenarios are assumed. The business as usual scenario (BAU) is a frozen scenario. There are two emission reduction scenarios (ERS and SRS) assumed under different emission reduction strength considering combined industrial process CO2 emission reduction approaches from both the production side and the consumption side. The results show that between 2020 and 2050, China’s lime industrial process has an increasingly significant CO2 emission reduction potential, enabling both emission intensity reductions and total emission reductions to be achieved simultaneously. Based on the simulation results from emission reduction scenarios, compared with 2012 level, in 2050, the emission intensity can be reduced by 13–27%, the total lime production can be reduced by 49–78%, and the CO2 emissions in the lime industrial process can be reduced by 57–85%.
The Potassium Channel Odyssey: Mechanisms of Traffic and Membrane Arrangement
Authors: Capera Jesusa , Serrano-Novillo Clara , Navarro-Pérez María , Cassinelli Silvia , Felipe Antonio
Published: February 2019

Ion channels are transmembrane proteins that conduct specific ions across biological membranes. Ion channels are present at the onset of many cellular processes, and their malfunction triggers severe pathologies. Potassium channels (KChs) share a highly conserved signature that is necessary to conduct K+ through the pore region. To be functional, KChs require an exquisite regulation of their subcellular location and abundance. A wide repertoire of signatures facilitates the proper targeting of the channel, fine-tuning the balance that determines traffic and location. These signature motifs can be part of the secondary or tertiary structure of the protein and are spread throughout the entire sequence. Furthermore, the association of the pore-forming subunits with different ancillary proteins forms functional complexes. These partners can modulate traffic and activity by adding their own signatures as well as by exposing or masking the existing ones. Post-translational modifications (PTMs) add a further dimension to traffic regulation. Therefore, the fate of a KCh is not fully dependent on a gene sequence but on the balance of many other factors regulating traffic. In this review, we assemble recent evidence contributing to our understanding of the spatial expression of KChs in mammalian cells. We compile specific signatures, PTMs, and associations that govern the destination of a functional channel.
Design of Novel Oligomeric Mixed Ligand Complexes: Preparation, Biological Applications and the First Example of Their Nanosized Scale
Authors: Okasha Rawda M. , AL-Shaikh Najla E. , Aljohani Faizah S. , Naqvi Arshi , Ismail Eman H.
Published: February 2019

A successful oligomerization of ternary metal complexes, cobalt (II), nickel (II), copper (II), zinc (II), chromium (III) and ferric sulfate (III) with nitrilotriacetic acid (NTA) as a primary ligand and glutamic acid as a secondary ligand, has been demonstrated. The formation of oligomers arose from the presence of the sulfate moiety, which operates as a bridged bidentate ligand that coordinates with other metal moieties. The novel oligomers exhibited octahedral structures, which bonded together through the sulfate moiety. In silico predictions were conducted to gauge the bioactivity, physico-chemical and pharmacokinetic properties. The biological activities of these oligomers as well as their tumor inhibitory behavior have been explored. This work also presents a facile and novel method of preparing these materials in nanosize, using Cetyltrimethylammonium bromide (CTAB) and polyvinyl alcohol (PVA) as capping ligands. The size and shape of the nanomaterials have been confirmed using the transmission electron microscope (TEM) and the scanning electron microscope (SEM).