bluuebunny/crossref_metadata_2025 huggingface dataset:
Occupational health of employees with mental health issues: the role of the psychosocial working conditions, job crafting, and sense of coherence
Authors: Lehmann Anja , Bauer Georg F.
Published: April 2024

BACKGROUND: The high prevalence of mental health issues (MHIs) among employees poses challenges for society. However, there is a lack of knowledge concerning the factors that influence their ability to stay, maintain productivity, and potentially thrive at work.OBJECTIVE: The aim of the study was to investigate individual (sense of coherence (SOC), job crafting), and work-related (psychosocial working conditions) factors that are associated with occupational health (work ability, work burnout and work engagement) among employees with MHIs controlling for socio-demographic and mental-health specific factors.METHODS: In a cross-sectional study employees with a current MHI from German-speaking countries were recruited. Hierarchical regression analyses with socio-demographic, mental-health specific, individual, and work-related factors as independent and occupational health outcomes as dependent variables were applied. RESULTS: The results showed that SOC and job resources were significantly related to all outcomes. In addition, job crafting tended to be associated only with positive outcomes (work ability and work engagement), whereas job demands only tended to be associated with burnout as a negative outcome. Moreover, mental health-specific factors (sickness absence and self-stigma) were negatively associated with work ability.CONCLISIONS: Personal resources (such as SOC) and job resources emerged as the most prominent factors for occupational health among employees with MHIs and should especially be strengthened in this group of individuals.
Global Care Policy Index 2022 Country Report: Sri Lanka
Authors: Sadanandan Shanya C. , Paul Anju Mary
Published: April 2024

Sri Lanka scored 3.42 out of 10 in the GCPI, placing it in the “Emerging” band. It does provide new mothers with generous breastfeeding breaks, and free prenatal and postnatal healthcare. However, Sri Lankan laws remain outdated as they have limited recognition of non-female family caregivers, and all maternity leave costs must be borne by the employer rather than being state-subsidized. Although Sri Lanka scored well for its protections for forced and underage domestic workers, it excludes domestic workers from almost all labor law coverage and protections on working conditions, remuneration, and fair employment.
Social housing pathways by policy co-design: opportunities for tenant participation in system innovation in Australia
Authors: Stone Wendy , Veeroja Piret , Goodall Zoë , Horton Ella , Duff Cameron
Published: April 2024

This AHURI research examines the participation of social housing tenants in developing social housing policy. With tenants increasingly presenting with more complex health, housing and social care needs, developing ways they can participate in social housing policy can lead to a range of positive benefits: from improving the way housing and associated essential social services are provided to giving tenants a heightened sense of autonomy and a stronger sense of belonging within their communities.The guiding principle for tenant participation is that those most affected by a policy or organisational decision ought to be involved in the decision making process. Internationally there is a relatively well-established understanding that complex systems, such as social housing systems, require the viewpoints of multiple stakeholders and that evidence-based policy making is best supported by including diverse voices such as lived experience experts and advocates.Successful tenant programs include understanding that tenants and housing providers can have different ideas of what participation should look like and what it should achieve; programs can be compromised by power imbalances between tenants and housing providers, which can limit tenant autonomy and also lead to conflict.For policy co-design to work well, there must be respect and recognition of the expertise of all participants involved in the policy making process, which may require workforce training and changing cultural norms.The research proposes sharing of best-practice examples of tenant participation and program practice guidelines between housing organisations and across sectors through a new Australian Housing Clearinghouse model.
स्वास्नीमान्छे उपन्यासको समाजशास्त्रीय अध्ययन
Authors: Silwal Ananda
Published: April 2024

हृदयचन्द्रसिंह प्रधानको स्वास्नीमान्छे उपन्यास सामाजपरक यथार्थमा आधारित प्रगतिवादी उपन्यास हो । प्रस्तुत आलेख प्रधानको स्वास्नीमान्छे उपन्यासको प्रजाति, पर्यावरण र क्षणगत प्रस्तुतिको विश्लेषणमा आधारित रहेको छ । प्रस्तुत उपन्यासको विविध कोणबाट अध्ययन भए पनि प्रजाति, पर्यावरण र क्षणका आधारमा अध्ययन भएको देखिँदैन । साहित्यको समाजशास्त्रीय मान्यताका आधारमा प्रधानको स्वास्नीमान्छे उपन्यासको अनुसन्धानमूलक अध्ययन नभएको सन्दर्भमा यसैअनुरूप व्यवस्थित ढाँचामा विश्लेषण गर्नु प्रस्तुत आलेखको मुख्य समस्या हो । प्रस्तुत मूल समस्याको समाधानार्थ प्रधानको स्वास्नीमान्छे उपन्यासमा प्रजाति, पर्यावरण र क्षणगत प्रस्तुति के–कस्तोे छ ? भन्ने प्राज्ञिक जिज्ञासाको उत्तरका लागि उनको औपन्यासिक कृतिको विश्लेषण गरी समस्यागत रिक्तताको परिपूर्ति गर्ने काम यसमा गरिएको छ । उनको औपन्यासिक कृतिको विविध अवधारणामा आधारित पूर्वकार्यको समीक्षा गरी अध्ययनको समस्यागत रिक्तताको खोजी गर्ने काम प्रस्तुत आलेखमा गरिएको छ । प्रस्तुत आलेखमा प्रधानको स्वास्नीमान्छे उपन्यासको विश्लेषण गर्नका लागि पुस्तकालयीय अध्ययन कार्यबाट सङ्कलित प्राथमिक तथा द्वितीयक स्रोतका सामग्रीको समष्टिगत गुणात्मक पद्धतिअनुरूप विश्लेषण गरिएको छ । साहित्यको समाजशास्त्रीय मान्यतामा आधारित प्रस्तुत अध्ययन आफैमा महत्त्वपूर्ण रहनुका साथै यसले सम्बद्ध क्षेत्रका अध्येतालाई प्राज्ञिक मार्गदर्शन गर्ने सन्दर्भमा थप औचित्यपूर्ण रहेको छ ।
‘बाघ मार्ने सियो’ लोककथाको विधातात्त्विक अध्ययन
Authors: Gyawali Shiva
Published: April 2024

यो लेख गुल्मी जिल्लाको गुल्मी दरबार गाउँपालिकामा प्रचलित ‘बाघ मार्ने सियो’ लोककथाको विधातात्त्त्विक अध्ययनमा केन्द्रित छ । कथाको कथानक सामाजिक विषयवस्तुसँग सम्बन्धित छ । कथाका चरित्रहरू मानवीय र मानवेत्तर पशुप्राणी तथा निर्जीव वस्तुहरू रहेका छन् । परिवेश सुखान्त प्रकृतिको रहेको छ । यो कथाले मनोरन्जन दिनुका अतिरिक्त मिलेर काम गरेमा जस्तोसुकै कठिनाइ पनि सजिलै पार गर्न सकिन्छ भन्ने सन्देश दिएको छ । भाषा सरल तथा बोधगम्य छ । रैखिक शैलीको कथामा वर्णनात्मक र संवादात्मक दुवै प्रकारको भाषिक विन्यास छ । अध्ययन गर्दा क्षेत्रकार्य गरेर तथ्याङ्क सङ्कलन गरिएको छ भने द्वितीयक स्रोतको प्रयोग गरेर विधातत्त्वको सिद्धान्त तयार गरिएको छ । त्यही सिद्धान्तका आधारमा यो कथाको विश्लेषण गरिएको छ । लेखलाई विषयपरिचय, अध्ययन विधि, लोककथाका तत्त्व, व्याख्या विश्लेषण, प्राप्ति र निष्कर्षमा विभाजित गरिएको छ । मौखिक परम्परामा रहेको लोककथाको अभिलेखीकरण पनि गरिएको छ ।
Osvrt na znanstveno-tehnički napredak, biomedicinu i etiku u medicini u časopisu Glasnik Hrvatskoga katoličkoga liječničkog društva
Authors: Tomić Draženko
Published: April 2024

Ovo istraživanje nastalo je na temelju razmatranja 61 priloga objavljenoga u časopisu Glasnik Hrvatskoga katoličkoga liječničkog društva (1991.–2021.), pri čemu su kao prediktori korišteni izrazi “znanstveno–tehnički napredak”, “biomedicina” i “etika u medicini”. Propitivan je suodnos moralnoga i znanstveno– tehničkoga napretka, dobrobiti i dobiti te ukazano na mogućnost suglasja oko minimuma općeprihvatljivoga vrijednosnoga sustava koji bi mogao biti posredovan i kroz bioetiku. Autor na svoj način izražava bojazan od rasčovječenja medicinske struke.
A importância da educomunicação no contexto da BNCC
Authors: Vieira da Silva Joseane Maria , Ghisleni Taís Steffenello , Carlesso Janaina Pereira Pretto
Published: April 2024

Atualmente, a mídia é um grande fator influenciável, negativa e positivamente na vida dos atores acadêmicos. Cabe à escola fazer o uso positivo desses recursos. Assim, esse artigo tem por objetivo justificar a inclusão de práticas da Educomunicação na política curricular para formação docente (Implementadores da BNCC). A pesquisa apresenta abordagem qualitativa, caráter documental e bibliográfico. O período de coleta de dados foi de 2020 a 2022, quando se ressaltou urgência de formação continuada para professores no letramento digital, irrompida a Covid-19. Foram pesquisadas nos repositórios eletrônicos SciELO, Google Acadêmico e Biblioteca Digital de Teses e Dissertações (BDTD): teses, artigos científicos e revistas eletrônicas que salientam políticas públicas para capacitação dos implementadores da BNCC, trabalhadas por Análise de Conteúdo (Bardin, 2006). Resultando, obteve-se autores como Baccega (1999), Moran (2000), Melo (2016) e Soares (2011-2018) no reforço ao labor docente através de novas metodologias educomunicativas.
Educação física e os esportes paralímpicos: experiência de adaptação para as aulas do ensino médio
Authors: Oliveira Mayla dos Santos de , Kawashima Larissa Beraldo
Published: April 2024

Os esportes paralímpicos são uma ferramenta muito importante de inclusão para sociedade, destacando-se no ambiente escolar como conteúdos da educação física. O objetivo deste relato de experiências é apresentar uma unidade didática aplicada para turmas do ensino médio integrado ao técnico em secretariado do Instituto Federal de Mato Grosso, bem como a avaliação da proposta pedagógica feita pelos estudantes participantes da pesquisa. A pesquisa foi dividida em três etapas: construção da proposta pedagógica; intervenção; e avaliação. A proposta buscou refletir sobre a inclusão social de pessoas com deficiência, trazendo o contexto histórico do esporte paralímpico, a sua importância, as diferentes modalidades, apresentou atletas paralímpicos e propôs aos estudantes analisarem os espaços da instituição e a possibilidade de mobilidade da pessoa com deficiência. Contribuiu com a formação dos estudantes, que futuramente poderão ser profissionais atuantes nas diferentes áreas da sociedade, pensando políticas públicas, colaborando com a reabilitação e educação para pessoas com deficiência.
Recepción artística en la enseñanza de artes visuales un estudio en los anales del ConFAEB
Authors: Díaz Hamlet Fernández
Published: April 2024

El trabajo expone un estudio de los Anales del Congreso Nacional de la Federación de Arte Educadores de Brasil (ConFAEB), realizado como parte de una investigación posdoctoral entre los años 2017 y 2019.  El objetivo consistió en analizar el tipo de investigaciones y reflexiones sobre la enseñanza de artes visuales con énfasis en la lectura de obras, en específico para el nivel Fundamental de la Educación Básica. Se revisaron los anales de cinco ediciones del ConFAEB, y se seleccionaron cincuenta ponencias que abordan el tema de la recepción del arte en función de procesos de enseñanza-aprendizaje en contexto escolar. Esos cincuenta trabajos fueron clasificados en cinco categorías con base en las coincidencias verificadas en los temas, objetivos, problemas y tipos de investigación. En este artículo presentamos los resultados del análisis de diecisiete ponencias clasificadas bajo la categoría “recepción de artes visuales en contexto escolar”, que representan el 34% del total de la muestra catalogada. Una de las conclusiones más importantes es que se detectó una asimetría entre las aspiraciones de los investigadores y arte-educadores, aquello que consideran importante y necesario para la enseñanza de artes visuales, y los problemas, obstáculos y carencias concretas que se expresan en la realidad de las prácticas educativas en contexto escolar. Dicha constatación nos pone ante una situación problémica que debe ser investigada en profundidad para así poder contribuir con posibles soluciones a la formación de los arte-educadores, en función de un tipo de enseñanza-aprendizaje de las artes visuales centrada en la recepción-comprensión de obras en contexto escolar.
DIFFERENT TYPES OF TRAINING’S POTENTIAL IN THE FORMATION OF ACTING SKILLS OF FUTURE SPECIALISTS OF SPECIALTY 026 PERFORMING ARTS
Authors: Soroka Olga , Baraniuk Vasyl
Published: April 2024

The article analyzes the potential of different types of training in the process of forming the acting skills for future students majoring in 026 Performing Arts. It has been determined that acting is a special state of experiencing. It is a role that can be achieved through training. The article summarizes the definition of the term “training”, which is an unconventional form of training organization, a way of organizing the activity of its participants with the aim of acquiring special knowledge and developing skills necessary for professional activity on this basis.Thus, psychophysical training helps to develop imaginative thinking, attention, a sense of a partner, distance, the ability to fix movements and plastically rebuild, creating a vivid image, accordingly forming the skill of an actor. Special attention is focused on actor training. It is understood as the process of improving the actor’s entire organism. It is done by adapting it to the increased requirements in practical work by playing systematic exercises that gradually become more difficult and increase the actor’s load.
ASPECTS OF ORGANIZATIONAL AND PEDAGOGICAL CONDITIONS OF GENDER CULTURE OF STUDENTS OF AGRICULTURAL INSTITUTIONS OF HIGHER EDUCATION
Authors: Shlieina Liudmyla
Published: April 2024

In this paper, the organizational and pedagogical conditions of the formation of gender competence of gender culture (GK) among students of agricultural institutions of higher education (HEIs) were investigated. The organizational and pedagogical conditions of the formation of gender competence of students of agricultural higher educational institutions are studied, the importance of including gender aspects in higher education and the need to understand the gender approach and gender relations in modern society are considered. Organizational and pedagogical conditions for the formation of gender culture of students of agricultural higher educational institutions are based on the scientific works of certain scientists who highlight the importance of education for the development of gender relations and general culture. Attention is drawn to the theoretical justification of the organizational and pedagogical conditions for the formation of gender competence. Various approaches and aspects of the organization of the educational process, methodical-methodological, socially useful, educational-communicative, and residential aspects have been studied. The article includes an analysis of documents and conceptual provisions of gender theory, which helps to determine the necessary approaches to the formation of gender competence. In particular, the authors consider the use of advanced educational methods and innovative technologies in the educational process. Calls for the creation of a favorable environment for the formation of gender competence of students of agricultural higher education institutions, which contributes to their personal and professional development, as well as gender equality in the educational environment. The systematic formation of gender competence of students of agricultural institutions of higher education in the context of their gender socialization during education was studied. Emphasis is placed on the change in ideas about gender roles and standards in modern society, which determines the relevance of a systemic approach to the formation of students’ gender competence. In conclusion, the article offers specific pedagogical conditions for the formation of gender competence of students of agricultural higher educational institutions. This includes the integration of gender issues into the educational process and the creation of a favorable environment for the development of gender awareness among future professionals.
DIGITAL COMPETENCE AND DIGITAL LITERACY OF HIGHER EDUCATION ACQUIRES
Authors: Zakharevych Mykola , Hryhorenko Vladyslav
Published: April 2024

The article examines the topical issue of digital skills formation among students of higher education institutions. The authors draw attention to the importance of digital competence and literacy in the modern digital world, their impact on educational processes and the future career of students. The article analyzes key aspects of digital competence, such as mastery of digital tools, critical thinking and the development of information literacy. It has been established that higher education students should have a targeted, activity-based, informative and effective learning system that will help them develop digital skills during their studies. It has been proven that digital competence is more than the ability to use the latest smartphones or computer software ‒ it is the ability to use such digital technologies critically and creatively. Information literacy is found to be a tool that uses the right technologies to find effective information and evaluate the results to prevent the data explosion, and the development of information literacy needs to be supported and use more information independently and with more confidence in a rapidly changing information society. This is not just mastering technical means, but also teaching critical thinking, information ethics and social responsibility. The formation of literacy in the field of digital competence accumulates the following abilities: knowledge of basic operational skills, the ability to search and evaluate information, the ability to process and analyze data, creativity and the ability to work together, awareness of information security and privacy protection.
PAEP gene restriction fragment length polymorphism and its effects on milk composition in cross-bred Hamdani sheep
Authors: Turgut Ali Osman , Gülendağ Erman , Koca Davut , Üner Sefa
Published: April 2024

β-lactoglobulin (βLG) stands as the primary whey protein in ruminant milk, synthesized by mammary gland cells during lactation and encoded by the βLG gene. This study aimed to assess the impact of βLG gene exon II polymorphism on milk composition traits in crossbreed Hamdani sheep. Sheep were examined for clinical diseases and mastitis. Milk and blood samples were only collected from healthy ewes. The composition and physical properties of milk were analyzed using milk autoanalyzer. The βLG gene exon II region's 452 bp PCR products were subjected to RFLP analysis using the RsaI restriction enzyme. Two genotypes, AA and AB, were identified for the βLG gene exon II region, with A and B allele frequencies of 0.7 and 0.3, respectively. Statistical analysis, conducted with Minitab® (Version: 19.2020.2.0), revealed that the AA genotype is associated with a higher milk fat percentage (p
Composition and Formation Conditions of Neoproterozoic Phosphorites of the Middle Urals
Authors: Krupenin M. T. , Kuznetsov A. B. , Zamyatin D. A. , Pankrushina E. A. , Lepekha S. V.
Published: April 2024

Phosphorite breccias compose a 6-m member in the Vendian terrigenous Kernos Formation in the basin of the Mezhevaya Utka and Sylvitsa rivers in the Middle Urals. Phosphorite pebbles and gravel are accumulations of fragments of redeposited crusts, originally formed in the early diagenesis near the surface of sandy-argillaceous deposits below the water-sediment boundary. Phosphorite is represented by fluorocarbonate apatite with unit cell parameter a from 0.9359 to 0.9363 nm, spectral mode parameters in Raman spectra (FWHM = 1–2 cm–1 and position from 963 to 966 cm–1), and a band at 1095 cm–1 in IR spectra. The absorption bands at ~1430 and 1453 cm–1 in the IR spectra correspond to the substitution of the orthophosphorus group by the carbonate ion (B-type). According to thermal analysis data, the CO2 content in apatite varies in the range of 0.04–0.8%, and the admixture of dispersed organic matter in the form of aliphatic compounds is 0.3–0.8%. Apatite is represented by two generations: primary in the form of basal structureless fine-crystalline cement and secondary in the composition of euhedral crystals up to 10 µm in size. Both generations corrode detrital quartz grains. Secondary apatite is enriched in P2O5, CaO, and F and contains less SiO2, FeO, Al2O3, MgO, and K2O than primary apatite. The PAAS-normalized REE distribution has a smoothed profile with a La/Yb ratio of about 2 and positive Ce- and Eu anomalies in enrichment phosphorites. The average value of F/P2O5 is 0.09 and corresponds to that in typical fluorocarbonate apatites that have undergone catagenesis. The high values of 87Sr/86Sr (from 0.7130 to 0.7253) in detrital phosphorites of the Kernos Formation suggest their deposition in a desalinated wave-dominated shallow-marine paleobasin near a significant inflow of continental waters or catagenetic recrystallization.
Chemical Weathering Indices and their Use for Paleoclimatic Reconstructions (on the Example of the Vendian‒Lower Cambrian Section of Podolsk Transnistria)
Authors: Maslov A. V. , Podkovyrov V. N.
Published: April 2024

The article provides an overview of various indices/indicators (Vogt, Parker, CIA, CIW, PIA, MIA, etc.) used in the study of weathering profiles/crusts and reconstruction of paleoclimatic environments of sedimentary sequence accumulation. Their possibilities are demonstrated by the example of Vendian-Lower Cambrian terrigenous deposits of Podolsk Transnistria (southwestern slope of the Ukrainian Shield). The distribution of the ba1 index values in this section indicates the presence of the material most transformed by the processes of chemical weathering in the mudstones of the Nagoryany Formation, the lower part of the Danylivka and the middle part of the Studenytsya formations. For mudstones of the Danylivka–Zbruch interval, the HM values are close to the HMPAAS. For rocks of the Yaryshiv–Nagoryany interval, the HM values are slightly higher, and for mudstones in the lower part of the Yaryshiv Formation, they are comparable to those inherent in continental clays of a hot tropical climate. The average SA index for mudstones of the entire section is 5.6 ± ± 0.7. Mudstones of the Hrushka–Nagoryany interval, where SA SAPAAS, are composed of more weathered material. The WIP values in the mudstones of the Mohyliv and Yaryshiv formations, as well as in the upper part of the Zbruch Formation, correspond to the interval of their values between PAAS and the average Archean granite. Clay rocks of other formations have WIP ≤ WIPPAAS values. The average CIA value for mudstones is 71 ± 4, which practically corresponds to the CIA value (70), which separates the sediments of cold/arid and warm/humid climates. Variations in the CIW index along the section are symbate with variations in the CIA. The vast majority of mudstones are characterized by PIA PIAPAAS values. The average CPA value is 91 ± 4, which is also typical for PAAS. The above and other data show that on the basis of a “direct” interpretation of the values of various indices of chemical weathering inherent in fine-grained clastic rocks, the paleoclimate that existed in the Vendian‒Early Cambrian on the territory of Podolsk Transnistria was rather moderate or warm humid. Comparison of the CIA values of mudstones with the values of this indicator for the particulate suspended matter of modern rivers suggests that the climate in the Vendian‒Early Cambrian resembled dry and humid subtropical or dry tropical with elements of humid.
Globular Phyllosilicates of the Glauconite-Illite Series in the Cambrian and Ordovican Rocks of the Eastern Baltica (North Estonia, Western Lithuania, Western Latvia)
Authors: Ivanovskaya T. A. , Sakharov B. A. , Zaitseva T. S.
Published: April 2024

The mineralogical, structural and crystal-chemical features of seven samples of globular phyllosilicates of the glauconite-illite series (GPS) from the Lower Cambrian of Northern Estonia and Western Lithuania, from the Middle Cambrian of Western Latvia, and also from the Lower Ordovician of Northern Estonia and Western Latvia are considered for the first time. The Al index GPS (КAl = VIAl/ [VIFe3+ + VIAl]) varies from 0.27 to 0.59, which allows us to attribute them to the glauconite-illite series, in which GPS are represented mainly by glauconites (the Al index is КAl = 0.27–0.46) and one sample of Al-glauconite (КAl = 0.59). The content of K2O in minerals is from 7.12 to 7.90%. For the first time, the content of expandable layers (4–13%), their types (smectite, vermiculite) and the character of their alternation (R = 0, R = 2) were determined in the studied samples by simulation of experimental X-ray diffraction patterns from oriented preparations. Simulation of X-ray diffraction patterns obtained from nonoriented preparations made it possible to reveal the degree of three-dimensional order, and mean values of the unit-cell parameter b (9.056–9.094 Å) as well as the features of the distribution of their individual micaceous varieties and to establish the heterogeneity of the samples. Based on these data, the microheterogeneity of the Cambrian and Ordovician GSSs was established and compared with the heterogeneity in the previously studied Riphean GPS [Drits et al., 2013]. The obtained Rb-Sr и K-Ar ages for the Middle Cambrian and Lower Ordovician samples, as well as the earlier published dates for the Lower Cambrian samples, are “rejuvenated” compared to age limits accepted for the Cambrian and Lower Ordovician [Gradstein et al., 2020]. The dependence of the Cambrian and Lower Ordovician “rejuvenated” isotopic dates and the discovered GPS heterogeneity, as well as possible reasons for its occurrence, are discussed.
New Data on the Age of Ozernoe Polymetallic Deposit (Western Transbaikal)
Authors: Minina O. R. , Gordienko I. V. , Damdinov B. B. , Tashlykov V. S. , Goneger T. A. , Skripnikov M. S. , Lantseva V. S. , Khubanov V. B. , Kislov E. V.
Published: April 2024

The article presents the results of biostratigraphic and U-Pb geochronological (detrital zircons) studies of the volcanic-terrigenous lacustrine member of the upper subformation of the Lower Paleozoic Oldyndinskaya Formation, which contains pyrite-polymetallic ores of the Ozernoye deposit (Kurbino-Eravninsky ore region, Western Transbaikalia). The first, second and “crystal tuff” productive horizons of the first ore-bearing level of the ozernaya member were studied. It is represented by an alternation of tuffs, calcareous, siliceous, carbonaceous tuffites, pelitomorphic limestones, limestone gravelstones with interlayers and lenses of mine-ralized tuff-conglobreccia and layers of banded siderite pyrite ores. For the first time, bryozoans, algae, and palynoflora were found in calcareous tuffaceous siltstones and limestones of the second and “crystalline tuff” productive horizons. These data indicate the Early Carboniferous, Tournaisian time of sediment accumulation. The results of U-Pb geochronological studies of detrital zircons from the mineralized tuff-conglobreccia of the third productive horizon limit the lower limit of the rock formation time as not older than the Late Cambrian.
Composition and Genesis of Carbonate Constructions in the Region of Paramushir Hydroacoustic Anomalies (Sea of Okhotsk)
Authors: Petrova V. V. , Rashidov V. A. , Gorkovа N. V.
Published: April 2024

Carbonate nodules from the area of the Paramushir hydroacoustic anomalies are territorially confined to the zones of methane seeps, controlled, in turn, by deep tectonically weakened bottom areas. According to the structure, chemical and mineral composition, the screeds can be classified as volcanogenic-sedimentary rock, consisting of vitro-, litho-, crystalline clasts and buried microfauna, cemented with carbonate (aragonite) cement. The details and sequence of mineral formation of carbonate nodules are considered.
Structural Heterogeneity and “Crystallinity” Indices of Natural Kaolinites
Authors: Drits V. A. , Sakharov B. A.
Published: April 2024

To overcome the existing uncertainty in the interpretation of “crystallinity” indices of kaolinites, HI [Hinckley, 1963], IK [Stoch, Sikora, 1966; Stoch, 1974], QF [Range, Weiss, 1969], AGFI [Aparicio, Galán, 1999; Aparicio et al., 2006], WIRI [Chmielová, Weiss, 2002], their values obtained for a representative collection of 30 kaolinite samples were compared with the results of modeling the corresponding X-ray diffraction patterns. It is shown that all the studied samples consist of a mixture of almost defect-free high ordered HOK and defective low-ordered LOK kaolinite phases, and that there are relationships between the HOK content and the values of the “crystallinity” indices, which are described by different regression equations. The relationship is most pronounced for HOK and the Hinckley index, HI, which is described by the quadratic equation HOK (%) = 12.236 HI2 + 25.464 HI ‒ 1.2622 with the correlation factor R2 = 0.993. The resulting equations can be used to find concentrations of HOK and LOK in natural kaolinites. Comparison of structural parameters of defective kaolinites obtained by modeling of their X-ray diffraction patterns with those of Expert System [Plançon, Zacharie 1990] showed that the latter sometimes predicts: 1) single-phase highly defective kaolinites, while their diffraction pattern modeling establishes a mixture of HOK and LOK phases; and 2) in two-phase samples, the content of the low-defect phase (ldp) is greater than 100%.
Structural and Сrystal-Сhemical Features of Mixed-Layer Illite-Containing Minerals from Diagenetically Altered Upper Jurassic Oil Source Rocks
Authors: Drits V. A. , Sakharov B. A. , Zviagina B. B.
Published: April 2024

Illite-smectite (I-S) minerals from the Upper Jurassic oil-bearing rock shales of Denmark and the North Sea were studied by a complex of diffraction and spectroscopic methods. Detailed structures were identified to reveal the mechanism of diagenetic transformation of these shales. Usually, in oil-bearing rocks of sedimentary basins, oil generation occurs simultaneously with the I-S diagenetic transformation. The results obtained demonstrate the relationship between these two reactions: NH3 molecules released from kerogen during ma-ximum oil formation are fixed as NH4 ammonium cations in smectite or vermiculite interlayers, forming mica or tobelite structural fragments. As a result of this solid-phase transformation, mixed-layer structures are formed, consisting of layers of illite, tobelite, smectite and vermiculite (I-T-S-V).
Continental Genesis of Sands of the Novolutsk Member, Sablinskaya Formation of the Middle Cambrian (Northwestern Flank of the Moscow Syneclise)
Authors: Platonov M. V. , Tugarova M. A.
Published: April 2024

Despite the high degree of knowledge about Cambrian-Ordovician sandy sequence in the northwestern flank of the Moscow syneclise, some of its subdivisions are still poorly studied. For the first time the article provides a comprehensive lithological analysis of the textural and mineral-structural composition of the Novolutsk member sands, common in the west of the Leningrad region. This member conventionally belongs to the Sablinskaya Formation of the Middle Cambrian. Significant differences between the studied sands and the sands of other units of the formation are shown, and their analogues are found in the eastern sections of the Baltic-Ladoga glint. The lenticular occurrence, specific textural complex and coarse-medium-grained composition of the Novolutsk unit sands made it possible to prove their continental alluvial genesis and link them with the sedimentary environments of the earlier identified Putilovskaya member sands of the Sablinskaya Formation.
Granulated Vivianite in the Cambridge Strait, Franz Josef Land (Barents Sea)
Authors: Murdmaa I. O. , Ovsepyan E. A. , Ivanova E. V. , Iakimova K. S.
Published: April 2024

Finding of vivianite is first described in a sediment core raised from the Cambridge Strait, western Franz Josef Land. The vivianite is represented by similar spherules mainly of 200–400 micrometers in diameter and by their rare aggregates. Distribution of the vivianite grains in the core is characterized by three maximal values (up to 2.7 grains per gram of dry bulk sediment), within the time interval of the last 4.1 kyr. Linear and flat shape of the aggregates indicates generation of vivianite at the sediment/water interface. It takes place in the reducing condition and sulfide sulfur deficit in the bottom water relative to bivalent iron. Structure of the vivianite grains varies from the cryptocrystalline porous to the fully crystalline dense one reflecting stages of the vivianite crystallization, likely after coagulation of the ferrous phosphate colloid formed due to the bacterial activity. Signs of vivianite microconcrections mentioned by some authors are not observed.
Composition Peculiarities of the Upper Devonian Coaly Rocks (The Sula River Section, North Timan)
Authors: Kotik O. S. , Pronina N. V. , Inkina N. S. , Beznosov P. A.
Published: April 2024

A detailed coal-petrographic and mineralogical-geochemical study of humic coals and coaly rocks in the Sargaev deposits of the Upper Devonian of the Northern Timan (Sula River), which is an example of the burial of the most ancient coal-forming plants, has been carried out. The following types of organic rocks are identified in the section: “jet-like” coal, liptobiolitic silty-clayey coal, and silty-clayey-coaly mixtolites. The studied coals and coaly rocks are composed of humic organic components, represented by vitrinite and liptinite macerals, the inertinite group is of subordinate importance. Vitrinite macerals include gelinite, collotelinite, and telinite (xylenite), liptinite macerals include sporinite, cutinite, liptodetrinite, bituminite, alginite, and a resinous substance, and inertinite macerals include inertodetrinite, semifusinite, fusinite, and funginite. The level of organic matter maturity according to the vitrinite reflectance RV = 0.45–0.55%, corresponds to the transition from proto- to meso-catagenesis (gradations PC3–MС1). The mineral component of mixtolites and high-ash coals consists of kaolinite, chlorite/smectite (with a predominance of the chlorite component) and quartz.
Sources of Material for Surface Bottom Sediments in Some Regions of the Nordic Seas (According to Geochemical Data)
Authors: Maslov A. V. , Politova N. V. , Klyuvitkin A. A. , Kozina N. V. , Kravchishina M. D. , Novigatsky A. N. , Novichkova E. A. , Alekseeva T. N. , Shevchenko V. P.
Published: April 2024

The mineral, bulk chemical (main rock-forming oxides) composition and systematics of rare-earth elements, Sc, Cr, Zr and Th of surface bottom sediments taken in a number of cruises of the R/V Akademik Mstislav Keldysh in the Nordic Seas (Mona, Knipovich, Kolbeinsey and Aegir ridges, the basins of the Norwegian and Greenland seas, the continental slope of Svalbard, the western continental margin of the Barents Sea, etc.) are considered to determine the sources of fine-grained material of bottom sediments. If for coastal sediments the primary influence of terrigenous drift is obvious, then seaward sediments are affected by “relatively war-mer” Atlantic waters, leading to their dilution with calcium carbonate, the main producers of which are planktonic foraminifers. It is concluded that the source of fine-grained aluminosiliciclastics for most of the studied samples were both acidic and basic igneous rocks (volcanic rocks of Iceland) in different proportions.
Structure and Evolution of the AqE Gene in Insects
Authors: Puzakova L. V. , Puzakov M. V.
Published: April 2024

AqE gene encodes a sulfolactate dehydrogenase-like enzyme of the LDH2/MDG2 oxidoreductase family. The gene was found in representatives of taxa of bacteria and fungi, as well as animals and plants whose lifestyle is associated with the aquatic environment. The AqE gene is also present in arthropods and, in particular, in the class of insects that are predominantly terrestrial. In our work, we studied the distribution and structure of the AqE gene in the class of insects in order to trace its evolutionary fate. We found that the studied gene is not present in all orders/suborders of insects, there is a loss of the gene. In some orders, it is duplicated or multiplied. The variability of the gene both in length and in exon-intron structure was established ‒ from intronless to multi-intron. It was found that the multiplication of the AqE gene of insects has an ancient nature, but there are also “young” duplications. It is possible that in connection with the appearance of paralogs, the gene acquired a new function.
DNA Probes for Analysis the Activity of Key Enzymes of the Base Excision DNA Repair Pathway in Human Cells
Authors: Alekseeva I. V. , Kuznetsova A. A. , Kladova O. A. , Shender V. O. , Schneider P. V. , Fedorova O. S. , Kuznetsov N. A.
Published: April 2024

The important role of DNA damage in the occurrence of various diseases, including cancer, stimulates studies of the mechanisms of genetic information stability, carried out since the discovery of DNA repair systems. The question of the relationship between the accumulation of DNA damage, disorders in DNA repair pathways, and an increased risk of diseases developing still remains relevant. Over the past few years, significant efforts have been made to develop methods for analyzing the activity of DNA repair enzymes in human cells. In this work, we developed fluorescent DNA probes that allow us to determine the activity of key enzymes of base excision DNA repair in cell extracts, namely DNA glycosylases UNG2, SMUG1, MBD4, TDG, AAG, NEIL1, NTHL1, and OGG1 and AP endonuclease APE1. The sensitivity of DNA probes was determined on purified enzyme preparations. Determination of the activity of repair enzymes in cell extracts of the human ovarian tumor lines TOV112, 79, OVCAR3, MESOV, SCOV3, and TOV21 revealed a significant variability in the level of enzyme activity in these cell lines. Obtained results can become the test system platform for analyzing the activity of the base excision DNA repair system in the human body.
Profiling of Targeted miRNAs (8-nt) for the Genes Involved in Type 2 Diabetes Mellitus and Cardiac Hypertrophy
Authors: Hussain K. , Ishtiaq A. , Mushtaq I. , Murtaza I.
Published: April 2024

Type 2 Diabetes Mellitus (T2DM) and cardiac hypertrophy (CH) are among the top ten leading cause of deaths, worldwide. T2DM and cardiac hypertrophy are the chronic diseases, have close association and direct life-threatening complications like stroke, myocardial infarction, retinopathy, nephropathy, and limb amputation. In addition to other medical approaches, miRNAs-based strategy is considered most efficient for early detection of chronic diseases and also has potential for the treatment of T2DM and cardiac hypertrophy like it is being used for cancer in clinical trials. MicroRNAs (miRNAs) are single stranded (non-coding) of 20 to 22 nucleotides sequences which bind to their target mRNA upon the complimentary basis, to silence the protein expression at post transcriptional level. Bioinformatic databases are used like online mendelian inheritance in man (OMIM), gene testing registry (GTR), TargetScan and ShinyGO for validation of disease linked genes and sorting the common miRNAs in both diseases, such as miR-30-5p/ 101-3p.2/ 190-5p/ 506-3p/ 9-5p/ 128-3p/ 137/ 96-5p/ 7-5p/ 107/ 101-3p.1/ 98-5p/ 124-3p.2/ 124-3p.1/ 16-5p/ 15-5p/ 497-5p/ 424-5p/ 195-5p/ 1271-5p, let-7-5p. Aforementioned databases were also used for the miRNAs which have more than one disease linked genes target in each pathological condition. Such miRNAs for cardiac hypertrophy are: miR-19-3p/ 183-5p.2/ 153-3p/ 372-3p/ 302-3p/ 520-3p/ 373-3p/ 129-5p/ 144-3p/ 139-5p and for T2DM are: miR-27-3p/ 206/ 1-3p/ 181-5p. This finding would be helpful for the appropriate selection of miRNAs and to design applicable research project in future. It will require more validation by using the miRNAs expression analysis, mimic, and anti-miRNA approach to check their potential against cardiac hypertrophy and T2DM.
New Zwitterionic Oligonucleotides: Preparation and Complementary Binding
Authors: Patrushev D. E. , Burakova E. A. , Bizyaev S. N. , Fokina A. A. , Stetsenko D. A.
Published: April 2024

New zwitter-ionic oligonucleotide derivatives containing 1,2,3,4-tetrahydroisoquinoline-7-sulfonyl phosphoramidate group are described. Automated synthesis of these compounds was carried out according to the β-cyanoethyl phosphoramidite scheme using the Staudinger reaction between 2-trifluoroacetyl-1,2,3,4-tetrahydroisoquinoline-7-sulfonyl azide and phosphite triester within an oligonucleotide grafted to a polymer support. 1,2,3,4-Tetrahydroisoquinoline-7-sulfonyl phosphoramidate group (THIQ) proved to be stable under the conditions of standard oligonucleotide synthesis, including the removal of protective groups and cleavage of the oligonucleotide from the polymer support by treatment with a mixture of concentrated aqueous solutions of ammonia and methylamine (1 : 1) at 55oC. Oligonucleotides modified by one to five THIQ groups in various positions were obtained. The zwitter-ionic character of the obtained derivatives was reflected in their different mobility under conditions of denaturing PAGE. The thermal stability of the duplexes of oligodeoxynucleotides containing THIQ groups with complementary DNA and RNA differed only slightly from that of natural DNA:DNA and DNA:RNA duplexes. The results obtained suggest the possible use of oligonucleotides modified with zwitterionic THIQ groups as antisense therapeutic agents.
Changes in the Expression of Genes, Associated with Calcium Processes, in the Hippocampus of Mice under the Influence of Chronic Social Defeat Stress
Authors: Pavlova M. B. , Smagin D. A. , Kudryavtseva N. N. , Dyuzhikova N. A.
Published: April 2024

Using data from a complete transcriptome analysis, changes in the expression of genes encoding proteins involved in calcium regulation processes in the hippocampus of male mice with symptoms of depression caused by chronic social defeat stress were investigated. The expression of Cacna1g, Cacnb3, Camk1g, Camk2d, Camk2n2, Caly, Caln1, S100a16, Slc24a4 genes in the hippocampus of depressed mice was increased in relation to control, while the Cacna2d1, Cacng5, Grin2a, Calm2 gene expression was reduced. The greatest number of significant correlations between the expression of the gene Calm2, which has the highest transcriptional activity, and other differentially expressed genes was revealed. It is assumed that calcium signaling in the hippocampus of mice is disrupted under the influence of chronic social defeat stress. The involvement of the Calm2 gene and the Сamk1g, Camk2d and Camk2n2 genes in this process is discussed.
Transcriptional Analysis of the Gene Encoding the Putative Myristoylated Membrane Protein (ORF458R) of Invertebrate Iridescent Virus 6 (IIV6)
Authors: Kuz C. A. , Ozsahin E. , Nalcacioglu R. , Demirbag Z.
Published: April 2024

Invertebrate iridescent virus 6 (IIV6) is a member of the genus Iridovirus and belongs to the Iridoviridae family. The entirely sequenced dsDNA genome, composed of 212 482 bp, encodes 215 putative open reading frames (ORFs). ORF458R encodes a putative myristoylated membrane protein. RT-PCR analysis of ORF458R expression in the presence of DNA replication and protein synthesis inhibitors showed that this gene is transcribed in the late phase of the virus infection. Time course analysis showed that transcription of ORF458R initiates between 12 and 24 h p.i. and starts to decrease after this point. Transcription of ORF458R initiated 53 nucleotides upstream of the translation start site and ended 40 nucleotides after the stop codon. Dual luciferase reporter gene assay showed that sequences between ‒61st and +18th nucleotides are essential for promoter activity. Interestingly, a remarkable decrease in promoter activity, in the presence of sequences between ‒299th and ‒143rd nucleotides, suggested a repressor activity between these regions. Our results showed that ORF458R is transcriptionally active, and separately located sequences at its upstream region with promoter and repressor activities regulating its expression. This information on the transcriptional analysis of ORF458R will contribute to our understanding of the molecular mechanisms of IIV6 replication.
Human rDNA Structure, Expression, and Non-Canonical Functions: the Role of Non-Coding Regions
Authors: Sadova A. A. , Panteleev D. Yu. , Pavlova G. V.
Published: April 2024

The review is dedicated to analyzing and summarizing the data on the part of human genome encoding 45S rRNA. The sequences which seem evolutionary conserved on the first glance astonish one with their variability in structure and a variety of functions on closer examination. The major part of rDNA is non-coding and contains regulatory elements, protein binding sites, pseudogenes, repetitive sequences, and microRNA genes. Ribosomal intergenic spacers are not only in charge with the nucleolus morphology and functioning, namely, the rRNA expression and ribosome biogenesis, but also control nuclear chromatin formation thus mediating cell differentiation. Besides, alterations in the expression of these non-coding regions of rDNA in response to environmental stimuli underlies the keen sense of cell to various types of stressors. Malfunctioning of this process may result in a wide range of pathologies from oncology to neurodegenerative disease and mental illness. Here we observe to-date materials on the structure and transcription of the ribosomal intergenic spacer in humans and its role in rRNA expression, in-born disease development, and cancer.
How Histone Deacetylase 3 Controls Hepcidin Expression and Hepatitis C Virus Replication
Authors: Shcherbakova А. S. , Kochetkov S. N. , Kozlov M. V.
Published: April 2024

The key role of histone deacetylases (HDACs) in the regulation of the cellular response to infection with the hepatitis C virus (HCV) was first demonstrated in 2008. Studying the metabolism of iron in the liver tissues of patients with chronic hepatitis C, the authors found that the expression of the hepcidin gene (HAMP), a hormone regulator of iron export, is markedly reduced in hepatocytes under conditions of oxidative stress caused by viral infection. HDACs were involved in the regulation of hepcidin expression through the control of acetylation level of histones and transcription factors, primarily STAT3, associated with the HAMP promoter. The purpose of this review is to summarize current data on the functioning of the HCV-HDAC3-STAT3-HAMP regulatory circuit as an example of a well-characterized interaction between the virus and the epigenetic apparatus of the host cell.
Methylation of Regulatory Regions of DNA Repair System Genes in Carotid Atherosclerosis
Authors: Babushkina N. P. , Zarubin A. A. , Koroleva Iu. A. , Gomboeva D. E. , Bragina E. Yu. , Goncharova I. A. , Golubenko M. V. , Salakhov R. R. , Sleptcsov A. A. , Kuznetsov M. S. , Kozlov B. N. , Muslimova E. F. , Afanasiev S. A. , Kucher A. N. , Nazarenko M. S.
Published: April 2024

The status of DNA methylation in the human genome changes during the pathogenesis of common diseases and acts as a predictor of life expectancy. Therefore, it is of interest to investigate the methylation level of regulatory regions of genes responsible for general biological processes that are potentially significant for the development of age-associated diseases. Among them there are genes encoding proteins of DNA repair system, which are characterized by pleiotropic effects. Here, results of the targeted methylation analysis of two regions of the human genome (the promoter of the MLH1 gene and the enhancer near the ATM gene) in different tissues of patients with carotid atherosclerosis are present. Analysis of the methylation profiles of studied genes in various tissues of the same individuals demonstrated marked differences between leukocytes and tissues of the vascular wall. Differences in methylation levels between normal and atherosclerotic tissues of the carotid arteries were revealed only for two studied CpG sites (chr11:108089866 and chr11:108090020, GRCh37/hg19 assembly) in the ATM gene. Based on this, we can assume the involvement of ATM in the development of atherosclerosis. “Overload” of the studied regions with transcription factor binding sites (according to ReMapp2022 data) indicate that the tissue-specific nature of methylation of the regulatory regions of the MLH1 and ATM may be associated with expression levels of these genes in a particular tissue. It has been shown that inter-individual differences in the methylation levels of CpG sites are associated with sufficiently distant nucleotide substitutions.