bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Tables 1-2 from IL6 Modulates the Immune Status of the Tumor Microenvironment to Facilitate Metastatic Colonization of Colorectal Cancer Cells
Authors: Toyoshima Yujiro , Kitamura Hidemitsu , Xiang Huihui , Ohno Yosuke , Homma Shigenori , Kawamura Hideki , Takahashi Norihiko , Kamiyama Toshiya , Tanino Mishie , Taketomi Akinobu
Published: April 2023

Supplementary Tables 1-2
Supplementary table 1 from Lactate-Mediated Acidification of Tumor Microenvironment Induces Apoptosis of Liver-Resident NK Cells in Colorectal Liver Metastasis
Authors: Harmon Cathal , Robinson Mark W. , Hand Fiona , Almuaili Dalal , Mentor Keno , Houlihan Diarmaid D. , Hoti Emir , Lynch Lydia , Geoghegan Justin , O'Farrelly Cliona
Published: April 2023

Antibodies and primers
Supplementary Figures 8-9 from IL6 Modulates the Immune Status of the Tumor Microenvironment to Facilitate Metastatic Colonization of Colorectal Cancer Cells
Authors: Toyoshima Yujiro , Kitamura Hidemitsu , Xiang Huihui , Ohno Yosuke , Homma Shigenori , Kawamura Hideki , Takahashi Norihiko , Kamiyama Toshiya , Tanino Mishie , Taketomi Akinobu
Published: April 2023

Supplementary Figures 8-9
Supplementary Figures 1-7 from IL6 Modulates the Immune Status of the Tumor Microenvironment to Facilitate Metastatic Colonization of Colorectal Cancer Cells
Authors: Toyoshima Yujiro , Kitamura Hidemitsu , Xiang Huihui , Ohno Yosuke , Homma Shigenori , Kawamura Hideki , Takahashi Norihiko , Kamiyama Toshiya , Tanino Mishie , Taketomi Akinobu
Published: April 2023

Supplementary Figures 1-7
Supplementary Table from CCR4 Blockade Depletes Regulatory T Cells and Prolongs Survival in a Canine Model of Bladder Cancer
Authors: Maeda Shingo , Murakami Kohei , Inoue Akiko , Yonezawa Tomohiro , Matsuki Naoaki
Published: April 2023

Supplementary Table from CCR4 Blockade Depletes Regulatory T Cells and Prolongs Survival in a Canine Model of Bladder Cancer
Supplementary Data from CCR4 Blockade Depletes Regulatory T Cells and Prolongs Survival in a Canine Model of Bladder Cancer
Authors: Maeda Shingo , Murakami Kohei , Inoue Akiko , Yonezawa Tomohiro , Matsuki Naoaki
Published: April 2023

Supplementary Data from CCR4 Blockade Depletes Regulatory T Cells and Prolongs Survival in a Canine Model of Bladder Cancer
Supplementary Data from Enhanced SLAMF7 Homotypic Interactions by Elotuzumab Improves NK Cell Killing of Multiple Myeloma
Authors: Pazina Tatiana , James Ashley M. , Colby Kimberly B. , Yang Yibin , Gale Andrew , Jhatakia Amy , Kearney Alper Y. , Graziano Robert F. , Bezman Natalie A. , Robbins Michael D. , Cohen Adam D. , Campbell Kerry S.
Published: April 2023

Supplementary Figures 1-9
Supplementary Data from Efficient Tumor Clearance and Diversified Immunity through Neoepitope Vaccines and Combinatorial Immunotherapy
Authors: Lee Karin L. , Benz Stephen C. , Hicks Kristin C. , Nguyen Andrew , Gameiro Sofia R. , Palena Claudia , Sanborn John Z. , Su Zhen , Ordentlich Peter , Rohlin Lars , Lee John H. , Rabizadeh Shahrooz , Soon-Shiong Patrick , Niazi Kayvan , Schlom Jeffrey , Hamilton Duane H.
Published: April 2023

Supplementary figures and tables
Supplementary Table S2 from Responsiveness to PD-1 Blockade in End-Stage Colon Cancer with Gene Locus 9p24.1 Copy-Number Gain
Authors: Ree Anne Hansen , Nygaard Vigdis , Russnes Hege G. , Heinrich Daniel , Nygaard Vegard , Johansen Christin , Bergheim Inger Riise , Hovig Eivind , Beiske Klaus , Negård Anne , Børresen-Dale Anne-Lise , Flatmark Kjersti , Mælandsmo Gunhild M.
Published: April 2023

Supplementary Table S2
Fig S4 from A Bispecific Molecule Targeting CD40 and Tumor Antigen Mesothelin Enhances Tumor-Specific Immunity
Authors: Ye Shiming , Cohen Diane , Belmar Nicole A. , Choi Donghee , Tan Siu Sze , Sho Mien , Akamatsu Yoshiko , Kim Han , Iyer Ramesh , Cabel Jean , Lake Marc , Song Danying , Harlan John , Zhang Catherine , Fang Yuni , Wahl Alan F. , Culp Patricia , Hollenbaugh Diane , Chao Debra T.
Published: April 2023

Effect of LB-1 in tumor model without MSLN expression
Supplementary Tables 1-2 from Blockade of β-Adrenergic Receptors Improves CD8+ T-cell Priming and Cancer Vaccine Efficacy
Authors: Daher Clara , Vimeux Lene , Stoeva Ralitsa , Peranzoni Elisa , Bismuth Georges , Wieduwild Elisabeth , Lucas Bruno , Donnadieu Emmanuel , Bercovici Nadège , Trautmann Alain , Feuillet Vincent
Published: April 2023

Tables S1 and S2
Supplementary Table 1 from Remodeling Translation Primes CD8+ T-cell Antitumor Immunity
Authors: Hurst Katie E. , Lawrence Kiley A. , Robino Rob A. , Ball Lauren E. , Chung Dongjun , Thaxton Jessica E.
Published: April 2023

Proteomics Table 1
Figure S1 from Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells
Authors: Wijewarnasuriya Dinali , Bebernitz Christina , Lopez Andrea V. , Rafiq Sarwish , Brentjens Renier J.
Published: April 2023

Sequences of CAR T cells with mCD8 signal peptide annotated in orange, scFv annotated in red, myc tag annotated in highlighted gray, mCD28 transmembrane and intracellular annotated in purple, mZeta annotated in green, IRES annotated in pink, mIL12 annotated in black, and m4-1BB annotated in yellow.
Table S4 from A CRISPR Screen Reveals Resistance Mechanisms to CD3-Bispecific Antibody Therapy
Authors: Liu Si-Qi , Grantham Alyssa , Landry Casey , Granda Brian , Chopra Rajiv , Chakravarthy Srinivas , Deutsch Sabine , Vogel Markus , Russo Katie , Seiss Katherine , Tschantz William R. , Rejtar Tomas , Ruddy David A. , Hu Tiancen , Aardalen Kimberly , Wagner Joel P. , Dranoff Glenn , D'Alessio Joseph A.
Published: April 2023

Table S4
Table S2 from MERTK Acts as a Costimulatory Receptor on Human CD8+ T Cells
Authors: Peeters Marlies J.W. , Dulkeviciute Donata , Draghi Arianna , Ritter Cathrin , Rahbech Anne , Skadborg Signe K. , Seremet Tina , Carnaz Simões Ana Micaela , Martinenaite Evelina , Halldórsdóttir Hólmfridur R. , Andersen Mads Hald , Olofsson Gitte Holmen , Svane Inge Marie , Rasmussen Lene Juel , Met Özcan , Becker Jürgen C. , Donia Marco , Desler Claus , thor Straten Per
Published: April 2023

Raw data sets from gene expression analysis.
Table S2 from A CRISPR Screen Reveals Resistance Mechanisms to CD3-Bispecific Antibody Therapy
Authors: Liu Si-Qi , Grantham Alyssa , Landry Casey , Granda Brian , Chopra Rajiv , Chakravarthy Srinivas , Deutsch Sabine , Vogel Markus , Russo Katie , Seiss Katherine , Tschantz William R. , Rejtar Tomas , Ruddy David A. , Hu Tiancen , Aardalen Kimberly , Wagner Joel P. , Dranoff Glenn , D'Alessio Joseph A.
Published: April 2023

Table S2
Table S3 from Immunologic Profiling of Mutational and Transcriptional Subgroups in Pediatric and Adult High-Grade Gliomas
Authors: Bockmayr Michael , Klauschen Frederick , Maire Cecile L. , Rutkowski Stefan , Westphal Manfred , Lamszus Katrin , Schüller Ulrich , Mohme Malte
Published: April 2023

Summary of functional signatures.
Supplementary Tables from Engineered Adoptive T-cell Therapy Prolongs Survival in a Preclinical Model of Advanced-Stage Ovarian Cancer
Authors: Anderson Kristin G. , Voillet Valentin , Bates Breanna M. , Chiu Edison Y. , Burnett Madison G. , Garcia Nicolas M. , Oda Shannon K. , Morse Christopher B. , Stromnes Ingunn M. , Drescher Charles W. , Gottardo Raphael , Greenberg Philip D.
Published: April 2023

Supplementary Tables
Figure S2 from Engineered Adoptive T-cell Therapy Prolongs Survival in a Preclinical Model of Advanced-Stage Ovarian Cancer
Authors: Anderson Kristin G. , Voillet Valentin , Bates Breanna M. , Chiu Edison Y. , Burnett Madison G. , Garcia Nicolas M. , Oda Shannon K. , Morse Christopher B. , Stromnes Ingunn M. , Drescher Charles W. , Gottardo Raphael , Greenberg Philip D.
Published: April 2023

Figure S2: Engineered murine T cells exhibit effector function in response to cognate peptide.
Supplementary Figures S1-5 from Alternative Splicing of the Inhibitory Immune Checkpoint Receptor SLAMF6 Generates a Dominant Positive Form, Boosting T-cell Effector Functions
Authors: Hajaj Emma , Zisman Elad , Tzaban Shay , Merims Sharon , Cohen Jonathan , Klein Shiri , Frankenburg Shoshana , Sade-Feldman Moshe , Tabach Yuval , Yizhak Keren , Navon Ami , Stepensky Polina , Hacohen Nir , Peretz Tamar , Veillette André , Karni Rotem , Eisenberg Galit , Lotem Michal
Published: April 2023

Supplementary Figures S1-5
Supplementary Data Figures from Improved T-cell Receptor Diversity Estimates Associate with Survival and Response to Anti–PD-1 Therapy
Authors: Bortone Dante S. , Woodcock Mark G. , Parker Joel S. , Vincent Benjamin G.
Published: April 2023

Combined supplementary figures with legends
Table S11 from Transient Depletion of CD4+ Cells Induces Remodeling of the TCR Repertoire in Gastrointestinal Cancer
Authors: Aoki Hiroyasu , Ueha Satoshi , Shichino Shigeyuki , Ogiwara Haru , Shitara Kohei , Shimomura Manami , Suzuki Toshihiro , Nakatsura Tetsuya , Yamashita Makiko , Kitano Shigehisa , Kuroda Sakiko , Wakabayashi Masashi , Kurachi Makoto , Ito Satoru , Doi Toshihiko , Matsushima Kouji
Published: April 2023

Supplementary Table 11
Table S1 from Chronic Adrenergic Stress Contributes to Metabolic Dysfunction and an Exhausted Phenotype in T Cells in the Tumor Microenvironment
Authors: Qiao Guanxi , Chen Minhui , Mohammadpour Hemn , MacDonald Cameron R. , Bucsek Mark J. , Hylander Bonnie L. , Barbi Joseph J. , Repasky Elizabeth A.
Published: April 2023

Supplementary table 1
Supplementary Table 1 from An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression
Authors: Sainson Richard C.A. , Thotakura Anil K. , Kosmac Miha , Borhis Gwenoline , Parveen Nahida , Kimber Rachael , Carvalho Joana , Henderson Simon J. , Pryke Kerstin L. , Okell Tracey , O'Leary Siobhan , Ball Stuart , Van Krinks Cassie , Gamand Lauriane , Taggart Emma , Pring Eleanor J. , Ali Hanif , Craig Hannah , Wong Vivian W.Y. , Liang Qi , Rowlands Robert J. , Lecointre Morgane , Campbell Jamie , Kirby Ian , Melvin David , Germaschewski Volker , Oelmann Elisabeth , Quaratino Sonia , McCourt Matthew
Published: April 2023

Genes used to define Immune cell subtypes
Figure S4 from Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML Development
Authors: Gui Xun , Deng Mi , Song Hao , Chen Yuanzhi , Xie Jingjing , Li Zunling , He Licai , Huang Fangfang , Xu Yixiang , Anami Yasuaki , Yu Hai , Yu Chenyi , Li Leike , Yuan Zihao , Xu Xiaoying , Wang Qihui , Chai Yan , Huang Tao , Shi Yi , Tsuchikama Kyoji , Liao X. Charlene , Xia Ningshao , Gao George F. , Zhang Ningyan , Zhang Cheng Cheng , An Zhiqiang
Published: April 2023

Phylogenetic analysis of D1 amino acid sequences of leukocyte immunoglobulin like receptors (LILRs).
Supplementary Figures and Table from TIGIT-Fc Promotes Antitumor Immunity
Authors: Shen Xian , Fu Wenyan , Wei Yongpeng , Zhu Junle , Yu Yue , Lei Changhai , Zhao Jian , Hu Shi
Published: April 2023

Supplementary Figures 1-5 and Supplementary Table 1
Supplementary Figures from Glycans as Immune Checkpoints: Removal of Branched N-glycans Enhances Immune Recognition Preventing Cancer Progression
Authors: Silva Mariana C. , Fernandes Ângela , Oliveira Maria , Resende Carlos , Correia Alexandra , de-Freitas-Junior Julio C. , Lavelle Aonghus , Andrade-da-Costa Jéssica , Leander Magdalena , Xavier-Ferreira Helena , Bessa José , Pereira Carina , Henrique Rui M. , Carneiro Fátima , Dinis-Ribeiro Mário , Marcos-Pinto Ricardo , Lima Margarida , Lepenies Bernd , Sokol Harry , Machado José C. , Vilanova Manuel , Pinho Salomé S.
Published: April 2023

Supplementary Figures S1-S7
Supplementary Figure 4 from DGKA Mediates Resistance to PD-1 Blockade
Authors: Fu Lingyi , Li Sen , Xiao WeiWei , Yu Kuai , Li Shuo , Yuan Sujing , Shen Jianfei , Dong Xingjun , Fang Ziqian , Zhang Jianeng , Chen Siyu , Li Wende , You Hua , Xia Xiaojun , Kang Tiebang , Tan Jing , Chen Gong , Yang An-Kui , Gao YuanHong , Zhou Penghui
Published: April 2023

Supplementary Figure 4
Supplementary Table S2 from Immunoregulation and Clinical Implications of ANGPT2/TIE2+ M-MDSC Signature in Non–Small Cell Lung Cancer
Authors: Lauret Marie Joseph Elodie , Laheurte Caroline , Jary Marine , Boullerot Laura , Asgarov Kamal , Gravelin Eléonore , Bouard Adeline , Rangan Laurie , Dosset Magalie , Borg Christophe , Adotévi Olivier
Published: April 2023

Effect of ANGPT2 on TAA-specific T cell responses.
Supplementary Table 2 from Targeting Triple-Negative Breast Cancer with Combination Therapy of EGFR CAR T Cells and CDK7 Inhibition
Authors: Xia Lin , Zheng Zaozao , Liu Jun-yi , Chen Yu-jie , Ding Jiancheng , Hu Guo-sheng , Hu Ya-hong , Liu Suling , Luo Wen-xin , Xia Ning-shao , Liu Wen
Published: April 2023

Supplementary Table 2
Supplementary Figure S2 from Immunoregulation and Clinical Implications of ANGPT2/TIE2+ M-MDSC Signature in Non–Small Cell Lung Cancer
Authors: Lauret Marie Joseph Elodie , Laheurte Caroline , Jary Marine , Boullerot Laura , Asgarov Kamal , Gravelin Eléonore , Bouard Adeline , Rangan Laurie , Dosset Magalie , Borg Christophe , Adotévi Olivier
Published: April 2023

Correlation between ANGPT2 and anti-WT1 T cell responses in NSCLC.
Figure S4 from Activin A Promotes Regulatory T-cell–Mediated Immunosuppression in Irradiated Breast Cancer
Authors: De Martino Mara , Daviaud Camille , Diamond Julie M. , Kraynak Jeffrey , Alard Amandine , Formenti Silvia C. , Miller Lance D. , Demaria Sandra , Vanpouille-Box Claire
Published: April 2023

INHBA expression correlates with the tumor-infiltrating Treg score in ER positive and negative breast cancer patients.
Supplementary Table 2 from Robust Antitumor Immunity in a Patient with Metastatic Colorectal Cancer Treated with Cytotoxic Regimens
Authors: Rajamanickam Venkatesh , Ballesteros-Merino Carmen , Samson Kimberly , Ross David , Bernard Brady , Fox Bernard A. , Tran Eric , Newell Pippa , Duhen Thomas
Published: April 2023

Supplementary Table 2
Supplementary Figure Legends from The Expression of Adenosine A2B Receptor on Antigen-Presenting Cells Suppresses CD8+ T-cell Responses and Promotes Tumor Growth
Authors: Chen Siqi , Akdemir Imran , Fan Jie , Linden Joel , Zhang Bin , Cekic Caglar
Published: April 2023

Supplementary Figure Legends
Supplementary Figure 8 from The Expression of Adenosine A2B Receptor on Antigen-Presenting Cells Suppresses CD8+ T-cell Responses and Promotes Tumor Growth
Authors: Chen Siqi , Akdemir Imran , Fan Jie , Linden Joel , Zhang Bin , Cekic Caglar
Published: April 2023

Supplementary Figure 8
Supplementary Table S2 from Longitudinal Immune Profiling Reveals Unique Myeloid and T-cell Phenotypes Associated with Spontaneous Immunoediting in a Prostate Tumor Model
Authors: Ager Casey R. , Obradovic Aleksandar Z. , Arriaga Juan M. , Chaimowitz Matthew G. , Califano Andrea , Abate-Shen Cory , Drake Charles G.
Published: April 2023

Supplementary Table S2
Supplementary Figure and Table from iPSC-Derived Neoantigen-Specific CTL Therapy for Ewing Sarcoma
Authors: Ishii Midori , Ando Jun , Yamazaki Satoshi , Toyota Tokuko , Ohara Kazuo , Furukawa Yoshiki , Suehara Yoshiyuki , Nakanishi Mahito , Nakashima Kazutaka , Ohshima Koichi , Nakauchi Hiromitsu , Ando Miki
Published: April 2023

Supplementary Figure 1 with legend and Supplementary Table 1
Table S4 from CD8+ T–cell Immune Surveillance against a Tumor Antigen Encoded by the Oncogenic Long Noncoding RNA PVT1
Authors: Kikuchi Yasuhiro , Tokita Serina , Hirama Tomomi , Kochin Vitaly , Nakatsugawa Munehide , Shinkawa Tomoyo , Hirohashi Yoshihiko , Tsukahara Tomohide , Hata Fumitake , Takemasa Ichiro , Sato Noriyuki , Kanaseki Takayuki , Torigoe Toshihiko
Published: April 2023

Table S4
Table S10 from CD8+ T–cell Immune Surveillance against a Tumor Antigen Encoded by the Oncogenic Long Noncoding RNA PVT1
Authors: Kikuchi Yasuhiro , Tokita Serina , Hirama Tomomi , Kochin Vitaly , Nakatsugawa Munehide , Shinkawa Tomoyo , Hirohashi Yoshihiko , Tsukahara Tomohide , Hata Fumitake , Takemasa Ichiro , Sato Noriyuki , Kanaseki Takayuki , Torigoe Toshihiko
Published: April 2023

Table S10
Supplementary Data from The Pellino1–PKCθ Signaling Axis Is an Essential Target for Improving Antitumor CD8+ T-lymphocyte Function
Authors: Park Jihyun , Lee Si-Yeon , Jeon Yoon , Kim Kyung-Mo , Lee Jin-Kwan , Ko Jiwon , Park Eun-Ji , Yoon Joon-Sup , Kang Baeki E. , Ryu Dongryeol , Lee Ho , Shin Su-Jin , Go Heounjeong , Lee Chang-Woo
Published: April 2023

Supplementary Data from The Pellino1–PKCθ Signaling Axis Is an Essential Target for Improving Antitumor CD8+ T-lymphocyte Function
Supplementary Figure Legends from PD-1/PD-L1–Associated Immunoarchitectural Patterns Stratify Pancreatic Cancer Patients into Prognostic/Predictive Subgroups
Authors: Karamitopoulou Eva , Andreou Andreas , Pahud de Mortanges Aurélie , Tinguely Marianne , Gloor Beat , Perren Aurel
Published: April 2023

Suppl. Figure Legends
Supplementary table from Accounting for B-cell Behavior and Sampling Bias Predicts Anti–PD-L1 Response in Bladder Cancer
Authors: Dyugay Ilya A. , Lukyanov Daniil K. , Turchaninova Maria A. , Serebrovskaya Ekaterina O. , Bryushkova Ekaterina A. , Zaretsky Andrew R. , Khalmurzaev Oybek , Matveev Vsevolod B. , Shugay Mikhail , Shelyakin Pavel V. , Chudakov Dmitriy M.
Published: April 2023

Supplementary table from Accounting for B-cell Behavior and Sampling Bias Predicts Anti–PD-L1 Response in Bladder Cancer
Supplementary Data from PBRM1 Inactivation Promotes Upregulation of Human Endogenous Retroviruses in a HIF-Dependent Manner
Authors: Zhou Mi , Leung Janet Y. , Gessner Kathryn H. , Hepperla Austin J. , Simon Jeremy M. , Davis Ian J. , Kim William Y.
Published: April 2023

Supplementary Data from PBRM1 Inactivation Promotes Upregulation of Human Endogenous Retroviruses in a HIF-Dependent Manner
Figure S7 from Integrin β7 Inhibits Colorectal Cancer Pathogenesis via Maintaining Antitumor Immunity
Authors: Zhang Youhua , Xie Ruting , Zhang Hailong , Zheng Yajuan , Lin Changdong , Yang Lei , Huang Mengwen , Li Man , Song Feifei , Lu Ling , Yang Muqing , Liu Ying , Wei Qing , Li Jiyu , Chen Jianfeng
Published: April 2023

Tumor tissue ITGB7 expression level represents the CD8+ T and B cell infiltration level, and the ITGB7 expression level on CD8+ T cells.
Figure S6 from Integrin β7 Inhibits Colorectal Cancer Pathogenesis via Maintaining Antitumor Immunity
Authors: Zhang Youhua , Xie Ruting , Zhang Hailong , Zheng Yajuan , Lin Changdong , Yang Lei , Huang Mengwen , Li Man , Song Feifei , Lu Ling , Yang Muqing , Liu Ying , Wei Qing , Li Jiyu , Chen Jianfeng
Published: April 2023

β7 blockade decreased the number of migrated CD3+ T cells towards CRC-organoids.
Figure S5 from Integrin β7 Inhibits Colorectal Cancer Pathogenesis via Maintaining Antitumor Immunity
Authors: Zhang Youhua , Xie Ruting , Zhang Hailong , Zheng Yajuan , Lin Changdong , Yang Lei , Huang Mengwen , Li Man , Song Feifei , Lu Ling , Yang Muqing , Liu Ying , Wei Qing , Li Jiyu , Chen Jianfeng
Published: April 2023

The expression pattern of α4β7 and αEβ7 in tumor infiltrating immune cells in WT mice bearing MC38 orthotopic tumor.
Figure S4 from Integrin β7 Inhibits Colorectal Cancer Pathogenesis via Maintaining Antitumor Immunity
Authors: Zhang Youhua , Xie Ruting , Zhang Hailong , Zheng Yajuan , Lin Changdong , Yang Lei , Huang Mengwen , Li Man , Song Feifei , Lu Ling , Yang Muqing , Liu Ying , Wei Qing , Li Jiyu , Chen Jianfeng
Published: April 2023

The correlation between ITGB7 expression and the expression of genes that are related to immunotherapy.
Figure S3 from Integrin β7 Inhibits Colorectal Cancer Pathogenesis via Maintaining Antitumor Immunity
Authors: Zhang Youhua , Xie Ruting , Zhang Hailong , Zheng Yajuan , Lin Changdong , Yang Lei , Huang Mengwen , Li Man , Song Feifei , Lu Ling , Yang Muqing , Liu Ying , Wei Qing , Li Jiyu , Chen Jianfeng
Published: April 2023

Differentially expressed gene and gene ontology analysis.
Movie 3 from Metabolic Screening of Cytotoxic T-cell Effector Function Reveals the Role of CRAC Channels in Regulating Lethal Hit Delivery
Authors: Slaats Jeroen , Dieteren Cindy E. , Wagena Esther , Wolf Louis , Raaijmakers Tonke K. , van der Laak Jeroen A. , Figdor Carl G. , Weigelin Bettina , Friedl Peter
Published: April 2023

OT-I CTL conjugation with B16F10/OVA cells in the absence (left) or presence (right) of 100 µM H2O2. Morgenstemning LUT, Fura-2 340/380-ratio. Time stamp, min:sec (0 min denoting the timepoint of contact initiation). Scale bar, 10 µm.
Movie 1 from Metabolic Screening of Cytotoxic T-cell Effector Function Reveals the Role of CRAC Channels in Regulating Lethal Hit Delivery
Authors: Slaats Jeroen , Dieteren Cindy E. , Wagena Esther , Wolf Louis , Raaijmakers Tonke K. , van der Laak Jeroen A. , Figdor Carl G. , Weigelin Bettina , Friedl Peter
Published: April 2023

OT-I CTL engagement with H2B-GFP-labeled MEC1/OVA cells followed by target cell death. The migration tracks and position of individual CTLs (numbers) are highlighted. Arrowheads highlight different phases of target cell death, including membrane blebbing, chromatin condensation, nuclear fragmentation and fading of GFP fluorescence (green color). Time stamp, h:min. Scale bar, 20 µm.
Supplementary Table from C3aR Signaling Inhibits NK-cell Infiltration into the Tumor Microenvironment in Mouse Models
Authors: Nandagopal Saravanan , Li Caiyun G. , Xu Yu , Sodji Quaovi H. , Graves Edward E. , Giaccia Amato J.
Published: April 2023

Supplementary Table
Figures S1-S5 from The SETDB1–TRIM28 Complex Suppresses Antitumor Immunity
Authors: Lin Jianhuang , Guo Dajiang , Liu Heng , Zhou Wei , Wang Chen , Müller Iris , Kossenkov Andrew V. , Drapkin Ronny , Bitler Benjamin G. , Helin Kristian , Zhang Rugang
Published: April 2023

Supplementary Figures S1-S5 + Legends
Movie S6 from CAR T-cell Entry into Tumor Islets Is a Two-Step Process Dependent on IFNγ and ICAM-1
Authors: Kantari-Mimoun Chahrazade , Barrin Sarah , Vimeux Lene , Haghiri Sandrine , Gervais Claire , Joaquina Sandy , Mittelstaet Joerg , Mockel-Tenbrinck Nadine , Kinkhabwala Ali , Damotte Diane , Lupo Audrey , Sibony Mathilde , Alifano Marco , Dondi Elisabetta , Bercovici Nadège , Trautmann Alain , Kaiser Andrew D. , Donnadieu Emmanuel
Published: April 2023

Ca2+ responses of Fluo-4-loaded EGFR CAR T cells in a vibratome section of a BxPC3 tumor
Movie S4 from CAR T-cell Entry into Tumor Islets Is a Two-Step Process Dependent on IFNγ and ICAM-1
Authors: Kantari-Mimoun Chahrazade , Barrin Sarah , Vimeux Lene , Haghiri Sandrine , Gervais Claire , Joaquina Sandy , Mittelstaet Joerg , Mockel-Tenbrinck Nadine , Kinkhabwala Ali , Damotte Diane , Lupo Audrey , Sibony Mathilde , Alifano Marco , Dondi Elisabetta , Bercovici Nadège , Trautmann Alain , Kaiser Andrew D. , Donnadieu Emmanuel
Published: April 2023

Ca2+ responses of Fura-2-loaded CD20 CAR T cells during their interaction with CD20-expressing BxPC3 tumor cells
Figure S4 from CAR T-cell Entry into Tumor Islets Is a Two-Step Process Dependent on IFNγ and ICAM-1
Authors: Kantari-Mimoun Chahrazade , Barrin Sarah , Vimeux Lene , Haghiri Sandrine , Gervais Claire , Joaquina Sandy , Mittelstaet Joerg , Mockel-Tenbrinck Nadine , Kinkhabwala Ali , Damotte Diane , Lupo Audrey , Sibony Mathilde , Alifano Marco , Dondi Elisabetta , Bercovici Nadège , Trautmann Alain , Kaiser Andrew D. , Donnadieu Emmanuel
Published: April 2023

Ca2+ responses of EGFR CAR T cells in human renal tumor slices positive for ICAM-1
Figure S3 from CAR T-cell Entry into Tumor Islets Is a Two-Step Process Dependent on IFNγ and ICAM-1
Authors: Kantari-Mimoun Chahrazade , Barrin Sarah , Vimeux Lene , Haghiri Sandrine , Gervais Claire , Joaquina Sandy , Mittelstaet Joerg , Mockel-Tenbrinck Nadine , Kinkhabwala Ali , Damotte Diane , Lupo Audrey , Sibony Mathilde , Alifano Marco , Dondi Elisabetta , Bercovici Nadège , Trautmann Alain , Kaiser Andrew D. , Donnadieu Emmanuel
Published: April 2023

CAR T cell-induced ICAM-1 expression by tumor cells facilitates lymphocyte activation and entry into tumor islets
Figure S2 from CAR T-cell Entry into Tumor Islets Is a Two-Step Process Dependent on IFNγ and ICAM-1
Authors: Kantari-Mimoun Chahrazade , Barrin Sarah , Vimeux Lene , Haghiri Sandrine , Gervais Claire , Joaquina Sandy , Mittelstaet Joerg , Mockel-Tenbrinck Nadine , Kinkhabwala Ali , Damotte Diane , Lupo Audrey , Sibony Mathilde , Alifano Marco , Dondi Elisabetta , Bercovici Nadège , Trautmann Alain , Kaiser Andrew D. , Donnadieu Emmanuel
Published: April 2023

ICAM-1 expression is associated with elevated T cell infiltration in human tumors
Supplementary Figure from Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma
Authors: Riedel Angela , Helal Moutaz , Pedro Luisa , Swietlik Jonathan J. , Shorthouse David , Schmitz Werner , Haas Lisa , Young Timothy , da Costa Ana S.H. , Davidson Sarah , Bhandare Pranjali , Wolf Elmar , Hall Benjamin A. , Frezza Christian , Oskarsson Thordur , Shields Jacqueline D.
Published: April 2023

Supplementary Figure from Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma
Supplementary Figures and Tables from Arid5a Promotes Immune Evasion by Augmenting Tryptophan Metabolism and Chemokine Expression
Authors: Parajuli Gyanu , Tekguc Murat , Wing James B. , Hashimoto Ari , Okuzaki Daisuke , Hirata Takeshi , Sasaki Atsushi , Itokazu Takahide , Handa Haruka , Sugino Hirokazu , Nishikawa Yoshihiro , Metwally Hozaifa , Kodama Yuzo , Tanaka Shinya , Sabe Hisataka , Yamashita Toshihide , Sakaguchi Shimon , Kishimoto Tadamitsu , Hashimoto Shigeru
Published: April 2023

Supplementary Figures 1-8 and Tables 1-3
Supplementary Figure from Insights into Immune Escape During Tumor Evolution and Response to Immunotherapy Using a Rat Model of Breast Cancer
Authors: Gil Del Alcazar Carlos R. , Trinh Anne , Alečković Maša , Rojas Jimenez Ernesto , Harper Nicholas W. , Oliphant Michael U.J. , Xie Shanshan , Krop Ethan D. , Lulseged Bethlehem , Murphy Katherine C. , Keenan Tanya E. , Van Allen Eliezer M. , Tolaney Sara M. , Freeman Gordon J. , Dillon Deborah A. , Muthuswamy Senthil K. , Polyak Kornelia
Published: April 2023

Supplementary Figure from Insights into Immune Escape During Tumor Evolution and Response to Immunotherapy Using a Rat Model of Breast Cancer
Supplementary Figure from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
Authors: Peng Sui , Chen Shuling , Hu Wei , Mei Jie , Zeng Xuezhen , Su Tianhong , Wang Wei , Chen Zebin , Xiao Han , Zhou Qian , Li Bin , Xie Yubin , Hu Huanjing , He Minghui , Han Yanyan , Tang Longqing , Ma Yifan , Li Xiaoshuang , Zhou Xiangjun , Dai Zihao , Liu Zelong , Tan Jiehui , Xu Lixia , Li Shaoqiang , Shen Shunli , Li Dongming , Lai Jiaming , Peng Baogang , Peng Zhenwei , Kuang Ming
Published: April 2023

Supplementary Figure from Combination Neoantigen-Based Dendritic Cell Vaccination and Adoptive T-Cell Transfer Induces Antitumor Responses Against Recurrence of Hepatocellular Carcinoma
Supplementary Table 1 from Immunomodulation of T- and NK-cell Responses by a Bispecific Antibody Targeting CD28 Homolog and PD-L1
Authors: Ramaswamy Madhu , Kim Taeil , Jones Des C. , Ghadially Hormas , Mahmoud Tamer I. , Garcia Andrew , Browne Gareth , Zenonos Zenon , Puplampu-Dove Yvonne , Riggs Jeffrey M. , Bhat Geetha K. , Herbst Ronald , Schofield Darren J. , Carlesso Gianluca
Published: April 2023

Supplementary Table 1
Suppl. Table 1 related to materials and methods
Supplementary Figures S1-S7 from Immunomodulation of T- and NK-cell Responses by a Bispecific Antibody Targeting CD28 Homolog and PD-L1
Authors: Ramaswamy Madhu , Kim Taeil , Jones Des C. , Ghadially Hormas , Mahmoud Tamer I. , Garcia Andrew , Browne Gareth , Zenonos Zenon , Puplampu-Dove Yvonne , Riggs Jeffrey M. , Bhat Geetha K. , Herbst Ronald , Schofield Darren J. , Carlesso Gianluca
Published: April 2023

Supplementary Figures S1-S7
Supplementary Figures S1-S7
Supplementary Figure from Hypophosphatemia Due to Increased Effector Cell Metabolic Activity Is Associated with Neurotoxicity Symptoms in CD19-Targeted CAR T-cell Therapy
Authors: Tang Jack Pengfei , Peters Cole W. , Quiros Crystal , Wang Xiaoyan , Klomhaus Alexandra M. , Yamada Reiko E. , Timmerman John M. , Moore Theodore B. , Nowicki Theodore S.
Published: April 2023

Supplementary Figure from Hypophosphatemia Due to Increased Effector Cell Metabolic Activity Is Associated with Neurotoxicity Symptoms in CD19-Targeted CAR T-cell Therapy
Supplementary Figure from Microenvironmental Landscape of Human Melanoma Brain Metastases in Response to Immune Checkpoint Inhibition
Authors: Alvarez-Breckenridge Christopher , Markson Samuel C. , Stocking Jackson H. , Nayyar Naema , Lastrapes Matt , Strickland Matthew R. , Kim Albert E. , de Sauvage Magali , Dahal Ashish , Larson Juliana M. , Mora Joana L. , Navia Andrew W. , Klein Robert H. , Kuter Benjamin M. , Gill Corey M. , Bertalan Mia , Shaw Brian , Kaplan Alexander , Subramanian Megha , Jain Aarushi , Kumar Swaminathan , Danish Husain , White Michael , Shahid Osmaan , Pauken Kristen E. , Miller Brian C. , Frederick Dennie T. , Hebert Christine , Shaw McKenzie , Martinez-Lage Maria , Frosch Matthew , Wang Nancy , Gerstner Elizabeth , Nahed Brian V. , Curry William T. , Carter Bob , Cahill Daniel P. , Boland Genevieve Marie , Izar Benjamin , Davies Michael A. , Sharpe Arlene H. , Suvà Mario L. , Sullivan Ryan J. , Brastianos Priscilla K. , Carter Scott L.
Published: April 2023

Supplementary Figure from Microenvironmental Landscape of Human Melanoma Brain Metastases in Response to Immune Checkpoint Inhibition
Supplementary Data from A Population of TIM4+FOLR2+ Macrophages Localized in Tertiary Lymphoid Structures Correlates to an Active Immune Infiltrate Across Several Cancer Types
Authors: Bugatti Mattia , Bergamini Marco , Missale Francesco , Monti Matilde , Ardighieri Laura , Pezzali Irene , Picinoli Sara , Caronni Nicoletta , Missolo-Koussou Yoann , Helft Julie , Benvenuti Federica , Vermi William
Published: April 2023

Supplementary Data from A Population of TIM4+FOLR2+ Macrophages Localized in Tertiary Lymphoid Structures Correlates to an Active Immune Infiltrate Across Several Cancer Types
Supplementary Table from BCG-Induced Tumor Immunity Requires Tumor-Intrinsic CIITA Independent of MHC-II
Authors: Redelman-Sidi Gil , Binyamin Anna , Antonelli Anthony C. , Catalano Will , Bean James , Al-Ahmadie Hikmat , Jungbluth Achim A. , Glickman Michael S.
Published: April 2023

Supplementary Table from BCG-Induced Tumor Immunity Requires Tumor-Intrinsic CIITA Independent of MHC-II
Supplementary Table from Tyrosine Kinase Inhibition Alters Intratumoral CD8+ T-cell Subtype Composition and Activity
Authors: Tieniber Andrew D. , Hanna Andrew N. , Medina Benjamin D. , Vitiello Gerardo A. , Etherington Mark S. , Liu Mengyuan , Do Kevin J. , Rossi Ferdinando , DeMatteo Ronald P.
Published: April 2023

Supplementary Table from Tyrosine Kinase Inhibition Alters Intratumoral CD8+ T-cell Subtype Composition and Activity
Supplementary Data from Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation
Authors: Edwards Kimberly J. , Chang Bryan , Babazada Hasan , Lohith Katheryn , Park Daniel H. , Farwell Michael D. , Sellmyer Mark A.
Published: April 2023

Supplementary Data from Using CD69 PET Imaging to Monitor Immunotherapy-Induced Immune Activation
Supplementary Figure from NET-Triggered NLRP3 Activation and IL18 Release Drive Oxaliplatin-Induced Peripheral Neuropathy
Authors: Lin Tongtong , Hu Liang , Hu Fan , Li Kun , Wang Chao-Yu , Zong Li-Juan , Zhao Ya-Qian , Zhang Xiaotao , Li Yan , Yang Yang , Wang Yu , Jiang Chun-Yi , Wu Xuefeng , Liu Wen-Tao
Published: April 2023

Supplementary Figure from NET-Triggered NLRP3 Activation and IL18 Release Drive Oxaliplatin-Induced Peripheral Neuropathy
Supplementary Figure from TGFβ and CIS Inhibition Overcomes NK-cell Suppression to Restore Antitumor Immunity
Authors: Souza-Fonseca-Guimaraes Fernando , Rossi Gustavo R. , Dagley Laura F. , Foroutan Momeneh , McCulloch Timothy R. , Yousef Jumana , Park Hae-Young , Gunter Jennifer H. , Beavis Paul A. , Lin Cheng-Yu , Hediyeh-Zadeh Soroor , Camilleri Tania , Davis Melissa J. , Huntington Nicholas D.
Published: April 2023

Supplementary Figure from TGFβ and CIS Inhibition Overcomes NK-cell Suppression to Restore Antitumor Immunity
Supplementary Figure from Gastric Microbiome Alterations Are Associated with Decreased CD8+ Tissue-Resident Memory T Cells in the Tumor Microenvironment of Gastric Cancer
Authors: Peng Rui , Liu Shuai , You Wenhua , Huang Yedi , Hu Chupeng , Gao Ye , Jia Xuemei , Li Gang , Xu Zekuan , Chen Yun
Published: April 2023

Supplementary Figure from Gastric Microbiome Alterations Are Associated with Decreased CD8+ Tissue-Resident Memory T Cells in the Tumor Microenvironment of Gastric Cancer
Supplementary Figure from BCG-Induced Tumor Immunity Requires Tumor-Intrinsic CIITA Independent of MHC-II
Authors: Redelman-Sidi Gil , Binyamin Anna , Antonelli Anthony C. , Catalano Will , Bean James , Al-Ahmadie Hikmat , Jungbluth Achim A. , Glickman Michael S.
Published: April 2023

Supplementary Figure from BCG-Induced Tumor Immunity Requires Tumor-Intrinsic CIITA Independent of MHC-II
Supplementary Figure from Adenosine A2a Receptor Antagonism Restores Additive Cytotoxicity by Cytotoxic T Cells in Metabolically Perturbed Tumors
Authors: Slaats Jeroen , Wagena Esther , Smits Daan , Berends Annemarie A. , Peters Ella , Bakker Gert-Jan , van Erp Merijn , Weigelin Bettina , Adema Gosse J. , Friedl Peter
Published: April 2023

Supplementary Figure from Adenosine A2a Receptor Antagonism Restores Additive Cytotoxicity by Cytotoxic T Cells in Metabolically Perturbed Tumors
Supplementary Figures from Protection of Regulatory T Cells from Fragility and Inactivation in the Tumor Microenvironment
Authors: Zhang Hongru , Tomar Vivek S. , Li Jinyang , Basavaraja Raghavendra , Yan Fangxue , Gui Jun , McBrearty Noreen , Costich Tara Lee , Beiting Daniel P. , Blanco M. Andres , Conejo-Garcia Jose R. , Saggu Gurpanna , Berger Allison , Nefedova Yulia , Gabrilovich Dmitry I. , Fuchs Serge Y.
Published: April 2023

Supplementary Figures 1-7 with legends
Supplemental Figures and Tables from Syntaphilin Regulates Neutrophil Migration in Cancer
Authors: Fu Shuyu , Deng Hui , Bertolini Irene , Perego Michela , Chen Eric S. , Sanseviero Emilio , Mostafa Ali , Alicea-Torres Kevin , Garcia-Gerique Laura , Stone Erica L. , Kossenkov Andrew V. , Schug Zachary T. , Nam Brian , Mulligan Charles , Altieri Dario C. , Nefedova Yulia , Gabrilovich Dmitry I.
Published: April 2023

Supplemental Figures, legends and tables
Supplementary Data from Efficacy and Synergy with Cisplatin of an Adenovirus Vectored Therapeutic E1E2E6E7 Vaccine against HPV Genome–Positive C3 Cancers in Mice
Authors: Boilesen Ditte Rahbæk , Neckermann Patrick , Willert Torsten , Müller Mikkel Dons , Schrödel Silke , Pertl Cordula , Thirion Christian , Asbach Benedikt , Wagner Ralf , Holst Peter Johannes
Published: April 2023

Supplementary Table 1, Supplementary Figures 1-7
Table S4 from Cancer Cell Resistance to IFNγ Can Occur via Enhanced Double-Strand Break Repair Pathway Activity
Authors: Han Tong , Wang Xujun , Shi Sailing , Zhang Wubing , Wang Jue , Wu Qiu , Li Ziyi , Fu Jingxin , Zheng Rongbin , Zhang Jiamin , Tang Qin , Zhang Peng , Wang Chenfei
Published: April 2023

Raw count table for RNA-seq.
Table S3 from Cancer Cell Resistance to IFNγ Can Occur via Enhanced Double-Strand Break Repair Pathway Activity
Authors: Han Tong , Wang Xujun , Shi Sailing , Zhang Wubing , Wang Jue , Wu Qiu , Li Ziyi , Fu Jingxin , Zheng Rongbin , Zhang Jiamin , Tang Qin , Zhang Peng , Wang Chenfei
Published: April 2023

Genes’ average Δβ-scores across all cancer cell lines (sheet1); Gene sets enrichment for positively (sheet2) / negatively (sheet3) selected genes in CRISPR screen. GSEA was used to perform the enrichment analysis; sgRNA sequences of AAVS1, PAXIP1, TP53BP1, XRCC4, RAD50, RAD51, BRCA1, BRCA2 (sheet4).
Supplementary Figures and Tables from CD8+ T cell–Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance
Authors: Lao Liyan , Zeng Wenfeng , Huang Penghan , Chen Huiping , Jia Zishuo , Wang Pei , Huang Di , Chen Jianing , Nie Yan , Yang Linbin , Wu Wei , Liu Jiang
Published: April 2023

Supplementary figures S1-S8 and tables S1-S6
Supplementary Figures from Tumor-Associated CD19+CD39− B Regulatory Cells Deregulate Class-Switch Recombination to Suppress Antibody Responses
Authors: Pati Subhadip , Mukherjee Sumon , Dutta Saikat , Guin Aharna , Roy Dia , Bose Sayantan , Paul Silpita , Saha Sudipto , Bhattacharyya Sankar , Datta Pratyush , Chakraborty Jayati , Sarkar Diptendra K. , Sa Gaurisankar
Published: April 2023

Supplementary Figures S1-S8
Supplementary Figure Legends from Extracellular Galectin 4 Drives Immune Evasion and Promotes T-cell Apoptosis in Pancreatic Cancer
Authors: Lidström Tommy , Cumming Joshua , Gaur Rahul , Frängsmyr Lars , Pateras Ioannis S. , Mickert Matthias J. , Franklin Oskar , Forsell Mattias N.E. , Arnberg Niklas , Dongre Mitesh , Patthey Cedric , Öhlund Daniel
Published: April 2023

Legends for sup figures 1-5
Supplementary Data from Reverse Translation Identifies the Synergistic Role of Immune Checkpoint Blockade and IL15 to Enhance Immunotherapy of Ovarian Cancer
Authors: Felices Martin , Wesley Erin , Bendzick Laura E. , Kodal Behiye , Hopps Rachel , Grzywacz Bartosz , Hinderlie Peter , Miller Jeffrey S. , Geller Melissa A.
Published: April 2023

Supplemental Table 1 and Supplemental Figures 1-5
Table S1, S2, S3, Figure S1, S2 from Low TCR Binding Strength Results in Increased Progenitor-like CD8+ Tumor-Infiltrating Lymphocytes
Authors: Hay Zachary L.Z. , Knapp Jennifer R. , Magallon Roman E. , O'Connor Brian P. , Slansky Jill E.
Published: April 2023

Flow cytometry panels, gating strategy and comparison of TILs to other gene signatures.
Supplementary Table 4 from Low TCR Binding Strength Results in Increased Progenitor-like CD8+ Tumor-Infiltrating Lymphocytes
Authors: Hay Zachary L.Z. , Knapp Jennifer R. , Magallon Roman E. , O'Connor Brian P. , Slansky Jill E.
Published: April 2023

Cluster ID Table
Supplementary Table Legends from Combination IFNβ and Membrane-Stable CD40L Maximize Tumor Dendritic Cell Activation and Lymph Node Trafficking to Elicit Systemic T-cell Immunity
Authors: Zheng Hong , Yu Xiaoqing , Ibrahim Mohammed L. , Foresman Dana , Xie Mengyu , Johnson Joseph O. , Boyle Theresa A. , Ruffell Brian , Perez Bradford A. , Antonia Scott J. , Ready Neal , Saltos Andreas N. , Cantwell Mark J. , Beg Amer A.
Published: April 2023

Supplementary Table Legends
Data from Urinary Bladder Cancer Tregs Suppress MMP2 and Potentially Regulate Invasiveness
Authors: Winerdal Malin E. , Krantz David , Hartana Ciputra A. , Zirakzadeh Ali A. , Linton Ludvig , Bergman Emma A. , Rosenblatt Robert , Vasko Janos , Alamdari Farhood , Hansson Johan , Holmström Benny , Johansson Markus , Winerdal Max , Marits Per , Sherif Amir , Winqvist Ola
Published: April 2023

Abstract
Regulatory T cells (Treg) have long been considered one-sided suppressors of antitumor immune responses and hence associated with poor patient outcome in cancer. However, evidence is mounting of a paradoxical positive prognostic effect of Tregs on certain malignancies, including urinary bladder cancer (UBC). This discrepancy has partly been attributed to the shear misidentification of Tregs, but also to the inflammatory profile of the tumor. Our aim was to determine whether tumor-infiltrating Forkhead box P3+ (FOXP3+) cells confer a stable Treg phenotype and to investigate putative beneficial Treg functions, focusing on tumor-promoting inflammatory pathways in UBC. Patients (n = 52) with suspected UBC were prospectively included. We show, by using a broad range of analytical approaches, that tumor-infiltrating CD4+FOXP3+ T cells in UBC phenotypically, functionally, and epigenetically represent a true Treg population. At the invasive front of UBC tumors, we found an inverse relationship between Treg frequency and expression of matrix metalloproteinase 2 (MMP2), a key proinvasive factor induced by tumor-promoting inflammation. Correspondingly, a significant, dose-dependent Treg-mediated downregulation of MMP2 protein and mRNA expression was observed in both macrophages and UBC cells. Also, we found that Treg frequency specifically at the invasive front positively correlated with survival. Thus, we identify Treg-mediated suppression of MMP2 in the tumor microenvironment as a mechanism explaining the paradoxical positive prognostic impact of tumor-infiltrating Tregs in UBC. Cancer Immunol Res; 6(5); 528–38. ©2018 AACR.
Data from Baseline Cytokine Profiles of Tuberculin-Specific CD4+ T Cells in Non–Muscle-Invasive Bladder Cancer May Predict Outcomes of BCG Immunotherapy
Authors: Jallad Samer , Thomas Philip , Newport Melanie J. , Kern Florian
Published: April 2023

Abstract
Intravesical Bacillus Calmette-Guérin (BCG) immunotherapy preserves the bladder after resection of high-risk non–muscle-invasive bladder cancer (NMIBC). About 30% of patients experience treatment failure, which cannot be predicted a priori and carries a high risk of disease progression. We examined the in vitro tuberculin responsiveness of CD4+ T cells before BCG immunotherapy in 42 patients with high-risk NMIBC. The frequencies and functionalities of cytokine-expressing CD4+ T cells immediately before and after BCG immunotherapy induction were assessed by flow cytometry after overnight tuberculin stimulation. Tuberculin-induced secreted mediators were measured by electrochemiluminescence. We correlated the results with recurrence-free patient survival 6 months after induction. A tuberculin-induced, secreted, IL2 concentration > 250 pg/mL was the best predictor of recurrence-free survival, providing 79% sensitivity, 86% specificity (AUC = 0.852, P = 0.000), and overall correct classification in 78.6% of cases. In 50% of patients later experiencing recurrence, but not in any of the recurrence-free survivors, IL2 secretion was < 120 pg/mL. Other parameters predicting recurrence-free survival included secreted IFNγ (AUC = 0.796, P = 0.002) and the frequencies of TNF-producing (TNF+) CD4+ T cells (AUC = 0.745, P = 0.010). “Polyfunctional” CD4+ T cells (IFNγ+/IL2+/TNF+) were significantly associated with recurrence-free survival (AUC = 0.801, P = 0.002). Thus, the amount of IL2 secretion from CD4+ T cells after overnight in vitro incubation with tuberculin predicted the outcome of BCG immunotherapy. As many as half of potential BCG failures could be identified before induction therapy is begun, enabling better choices regarding treatment. Cancer Immunol Res; 6(10); 1212–9. ©2018 AACR.
Data from Antibody-Neutralized Reovirus Is Effective in Oncolytic Virotherapy
Authors: Berkeley Robert A. , Steele Lynette P. , Mulder Aat A. , van den Wollenberg Diana J.M. , Kottke Timothy J. , Thompson Jill , Coffey Matthew , Hoeben Rob C. , Vile Richard G. , Melcher Alan , Ilett Elizabeth J.
Published: April 2023

Abstract
Immunotherapy is showing promise for otherwise incurable cancers. Oncolytic viruses (OVs), developed as direct cytotoxic agents, mediate their antitumor effects via activation of the immune system. However, OVs also stimulate antiviral immune responses, including the induction of OV-neutralizing antibodies. Current dogma suggests that the presence of preexisting antiviral neutralizing antibodies in patients, or their development during viral therapy, is a barrier to systemic OV delivery, rendering repeat systemic treatments ineffective. However, we have found that human monocytes loaded with preformed reovirus–antibody complexes, in which the reovirus is fully neutralized, deliver functional replicative reovirus to tumor cells, resulting in tumor cell infection and lysis. This delivery mechanism is mediated, at least in part, by antibody receptors (in particular FcγRIII) that mediate uptake and internalization of the reovirus/antibody complexes by the monocytes. This finding has implications for oncolytic virotherapy and for the design of clinical OV treatment strategies. Cancer Immunol Res; 6(10); 1161–73. ©2018 AACR.
Data from Association Studies of Fcγ Receptor Polymorphisms with Outcome in HER2+ Breast Cancer Patients Treated with Trastuzumab in NCCTG (Alliance) Trial N9831
Authors: Norton Nadine , Olson Rebecca M. , Pegram Mark , Tenner Kathleen , Ballman Karla V. , Clynes Raphael , Knutson Keith L. , Perez Edith A.
Published: April 2023

Abstract
Patients with HER2+ breast cancer treated with trastuzumab and chemotherapy have superior survival compared with patients treated with chemotherapy alone. Polymorphisms within FCGR2A and FCGR3A are associated with binding affinity of natural killer cells to the IgG1 portion of trastuzumab, and a polymorphism in FCGR2B (I232T) is associated with impaired regulatory activity. The association of these polymorphisms with clinical response among trastuzumab-treated patients is equivocal, with both positive and negative associations. We performed genotyping analysis on the FCGR3A V158F, FCGR2A R131H, and FCGR2B I232T polymorphisms in 1,325 patients from the N9831 clinical trial. Patients in arm A (N = 419) received chemotherapy only. Patients in arms B (N = 469) and C (N = 437) were treated with chemotherapy and trastuzumab (sequentially in arm B and concurrently in arm C). Using log-rank test and Cox proportional hazard models, we compared disease-free survival (DFS) among genotypic groups within pooled arms B/C. We found no differences in DFS between trastuzumab-treated patients who had the FCGR3A 158 V/V and/or FCGR2A 131 H/H high-affinity genotypes and patients without those genotypes. Furthermore, there was no significant interaction between FCGR3A and FCGR2A and treatment. However, there was a difference in DFS for FCGR2B I232T, with I/I patients deriving benefit from trastuzumab (P < 0.001), compared with the T carriers who did not (P = 0.81). The interaction between FCGR2B genotype and treatment was statistically significant (P = 0.03). Our analysis did not reveal an association between FcγR high-affinity genotypes and outcomes. However, it seems that the FCGR2B inhibitory gene may be predictive of adjuvant trastuzumab benefit. Cancer Immunol Res; 2(10); 962–9. ©2014 AACR.