bluuebunny/crossref_metadata_2025 huggingface dataset:
Intrathecal immunoglobin synthesis and its role in patients with neurosyphilis
Authors: Huang Xiyue , Ying Shanshan , Luo Lan , Li Lixin , Li Dongdong , Xie Yi
Published: November 2022

Background

Intrathecal protein synthesis (ITS) occurs in various central nervous system disorders, but few quantitative studies have focused on ITS for neurosyphilis (NS) in southwestern China. We made a study to quantitatively assess the ITS in patients with NS and to investigate the association between ITS and the stages of NS.

Methods

CSF–serum specimen pairs from 142 patients (66 NS and 76 non-NS/syphilis) were collected for routine CSF and serum tests. The NS group was divided into slight and severe subgroups according to the NS stages. Three formulas for the quantitative determination of the intrathecal synthesis were calculated to characterize the specimens, including the Ig index (QIg/Qalb), Ig extended index (Ig_EI), and intrathecally synthesized fraction (IgIF) using the hyperbolic function. The role of QTPPA/QIgG as an antibody index (AI = Q specific Ig/QIgG) was also explored.

Results

Sero_TRUST titres (1:16, 1:1-1:256), sero_TPPA titres (1:163840, 1:1280-1:1310720), total protein (MTP), and CSF_Igs (p < 0.05) were found to be significantly elevated in the NS group. Intrathecal Ig synthesis can be identified using all three formulas in the NS group. The pattern of Ig intrathecal synthesis was IgIF-G (48.62%) > IgIF-A = IgIF-M (p < 0.05), with the dominant intrathecal fraction being IgG (median, 48.62%), which was also verified by QIgG> Qalb> QIgM = QIgA. In the slight NS group, the intrathecal fractions of IgM (>0 in 4 out of 20 cases) and IgG (>0 in 16 out of 20) were lower than the intrathecal fractions of IgM (>0 in 19 out of 35 cases) and IgG (>0 in 33 out of 38) in the severe group (p < 0.05). The area under the curve (AUC) of the CSF_TPPA antibody index was 0.867 (0.792, 0.922), with an optimal cutoff point of 0.81, providing a sensitivity of 88.91% and specificity of 84.62%.

Conclusion

Although the intrathecal synthesis pattern is IgG dominant in patients with NS, brain-derived IgM and IgA can also be found. Moreover, intrathecal IgM and IgG were associated with a parenchymatous type of neurosyphilis. Syphilis-specific antibodies are a new potential tool for NS diagnosis.
Encouraging physicians' continuous knowledge-sharing in online health communities: A motivational perspective
Authors: Zhang Xin , Gao Shanzhen , Cheng Yanyan , Meng Fanbo
Published: November 2022

Online health communities (OHCs) as an essential means of patient education can significantly improve patients' health literacy and treatment outcomes. However, sustaining these social benefits brought by OHCs establishes the prerequisite that physicians can continuously share their knowledge on OHCs. Although previous studies have explored physicians' knowledge-sharing in OHCs, scholarly knowledge related to the means of motivating physicians to continue sharing their knowledge remains limited. Therefore, this study developed a research model based on motivation theory to explore the influence of practical benefits, psychological rewards, and perceived connectedness with OHCs on physicians' continuous knowledge-sharing behaviors and the contingent role of physicians' online seniority status. The research model and relevant hypotheses were examined using objective data from one of the leading OHCs in China. The empirical results reveal that both practical benefits and psychological rewards positively affect physicians' continuous knowledge-sharing behaviors. However, an unexpected finding is that perceived connectedness is negatively associated with physicians' continuous knowledge-sharing behaviors. In addition, physicians' online seniority status strengthens the relationship between practical benefits and continuous knowledge-sharing behaviors but weaken the role of psychological rewards and perceived connectedness on continuous knowledge-sharing behaviors. This study contributes to the understanding of the motivational mechanisms underlying physicians' continuous knowledge-sharing behaviors in OHCs and provides significant practical implications for practitioners of OHCs.
Association of metabolic dysfunction-associated fatty liver disease, type 2 diabetes mellitus, and metabolic goal achievement with risk of chronic kidney disease
Authors: Su Weitao , Chen Minhui , Xiao Ling , Du Shanshan , Xue Lihua , Feng Ruimei , Ye Weimin
Published: November 2022

Background

Although type 2 diabetes mellitus (T2DM) plays a significant role in the association between metabolic dysfunction-associated fatty liver disease (MAFLD) and chronic kidney disease (CKD), how T2DM development and glycemic deterioration affect CKD and its renal function indicators, estimated glomerular filtration rate (eGFR) and urine albumin-to-creatinine ratio (UACR), remains unknown. We aimed to assess the association between MAFLD, along with T2DM, and risk of CKD, and then evaluate the effect of metabolic goal achievement in MAFLD on the risk of CKD.

Methods

In this cross-sectional study, 5,594 participants were included. Multivariate logistic regression and linear regression were used to examine the association between MAFLD with its T2DM status and metabolic goal achievement and risk of CKD, as well as eGFR and UACR.

Results

The MAFLD group had a higher prevalence of CKD (16.2 vs. 7.6%, P < 0.001) than the non-MAFLD group. MAFLD was independently associated with an increased risk of CKD (odds ratio [OR]: 1.35, 95% CI: 1.09–1.67) and increased eGFR and UACR. Among the three MAFLD subtypes, only the T2DM subtype exhibited significant associations with increased risk of CKD (OR: 2.85, 95% CI: 2.24–3.63), as well as increased eGFR and UACR. Glycemic deterioration in MAFLD was dose-dependently associated with an increased risk of CKD (P-trend < 0.001). Achieved metabolic goals in MAFLD decreased the risk of CKD, eGFR, and UACR; MAFLD with 2 or 3 achieved metabolic goals was not significantly associated with the risk of CKD (OR: 0.81, 95% CI: 0.59–1.12) and albuminuria.

Conclusion

MAFLD was independently associated with an increased risk of CKD, as well as increased eGFR and UACR. This association is strongly driven by T2DM status. Glycemic deterioration in MAFLD was dose-dependently associated with an increased risk of CKD. Achieved metabolic goals in MAFLD decreased the risk of CKD by reducing the risk of albuminuria.
Association between social support and medication literacy in older adults with hypertension
Authors: Shen Zhiying , Ding Siqing , Shi Shuangjiao , Zhong Zhuqing
Published: November 2022

Background

Reduced physical function and reduced social networks place older adults with hypertension at high risk for medication-related harm. Medication literacy is one of the preventable factors that affect the success of drug therapy for hypertension. However, little is known about the level of medication literacy and its influencing factors in older adults with hypertension.

Objective

The purpose of this study was to investigate the levels of social support and medication literacy, and the association between them in older Chinese adult patients with hypertension.

Methods

A total of 362 older adult patients with hypertension were investigated using a demographic characteristics questionnaire, the Chinese Medication Literacy Scale for Hypertensive Patients (C-MLSHP) and the Social Support Rating Scale (SSRS). Pearson correlation analysis, canonical correlation analysis (CCA) and hierarchical linear regression were used to analyse the relationship between social support and medication literacy.

Results

Our results showed that the mean scores for the C-MLSHP and the SSRS for older adult patients with hypertension were 23.89 (SD = 4.66) and 39.22 (SD = 5.53), respectively. The results of the Pearson correlation analysis suggested that the score for social support was positively correlated with the score for medication literacy (r = 0.431, P < 0.01). The results of CCA demonstrate that older adult patients with hypertension who had more subjective (rs = 0.682) and objective support (rs = 0.817) performed better in knowledge (rs = 0.633), skills (rs = 0.631) and behavior literacy (rs = 0.715). Hierarchical linear regression indicated that two dimensions of subjective support (B = 0.252, P < 0.001) and objective support (B = 0.690, P < 0.001) in social support were found to be independent predictors of medication literacy (R2 = 0.335, F = 19.745, P < 0.001).

Conclusion

Social support is positively associated with medication literacy in older Chinese adult patients with hypertension. The study highlights the importance of social support in promoting medication literacy among older adult patients with hypertension.
Late effects of heavy-ion space radiation on splenocyte subpopulations and NK cytotoxic function
Authors: Leung Calvin N. , Howell Donna M. , De Toledo Sonia M. , Azzam Edouard I. , Howell Roger W.
Published: November 2022

Introduction: With current goals of increased space exploration and travel to Mars, there has been great interest in understanding the long-term effects of high atomic number, high energy (HZE) ion exposure on various organ systems and the immune system. Little is known about late effects on the immune system after HZE exposure. Therefore, our objective was to determine how natural killer (NK) cell populations were affected in geriatric mice that were exposed to HZE particles during middle-age, thereby representing elderly retired astronauts that undertook deep space missions.

Methods: 10 month old male CBA/CaJ mice were whole-body irradiated: sham (control); 150-cGy gamma-rays (delivered in 1 fraction); 40-cGy 1-GeV/nu 28Si14+ ions (delivered in 3 fractions); 40-cGy 1-GeV/nu 16O8+ ions (1 fraction); and 40-cGy 1-GeV/nu 16O8+ ions (3 fractions). The mice were sacrificed 1–1.5 yr post-exposure, and the spleens harvested. Splenocyte effector (E) cells were harvested and added to 51Cr-labeled Yac-1 target (T) cells in E:T ratios of 12:1, 25:1, 50:1, and 100:1. NK cytotoxicity was measured with 51Cr release. In addition, 2 million splenocytes were aliquoted and stained with a seven-antibody cocktail, and flow cytometry was used to determine the percentage of NK, B lymphocytes, and T lymphocytes in the splenocyte population.

Results: Mice exposed to either a single fraction of 150-cGy gamma rays or 40-cGy 16O8+ ions in 3 fractions were found to have significant decreases in NK cytotoxicity of approximately 30% and 25%, respectively. No significant differences were observed in NK cytotoxicity for 40-cGy 16O8+ ions delivered in 1 fraction, or 40-cGy 28Si14+ ions delivered in 3 fractions. No significant differences were observed in the percentage of spleen cells that were NK (%NK) amongst the groups.

Conclusion: Fractionated HZE ion exposure has the potential to affect the innate arm of the immune system long after exposure, leading to decreases in NK cell function. Therefore, protective countermeasures may need to be considered to decrease the risk of reduced long-term immune function in elderly retired astronauts that undertook deep space missions.
Land-use and food security in energy transition: Role of food supply
Authors: Zhuang Daoyong , Abbas Jaffar , Al-Sulaiti Khalid , Fahlevi Mochammad , Aljuaid Mohammed , Saniuk Sebastian
Published: November 2022

Food security in a just energy transition is a growing debate about designing sustainable food secure networks worldwide. Energy transition, land-use change, and food security are crucial factors for food security and provision. The increased demand for food products and customer preferences regarding food safety provide various issues for the current agriculture food supply chain (AFSC). Along with rising sustainability concerns, strict government regulation, food security, and traceability concerns compel managers, business houses, and practitioners working in AFSC to adopt new tools, techniques, and methodologies to model current food supply chain problems. Thus, in turn, design the food logistics network for food security. Hence, this study investigates the core determinants of food security and supply in Egypt, Morocco, Tunisia, and Lebanon over the period of 2010–2019. In order to estimate the objectives of the study, we employ the fully modified ordinary least square (FMOLS) and dynamic ordinary least squares estimators (DOLS) to draw the study findings. However, the estimated results show a negative association of land use with food security and supply. Likewise, energy transition, gross domestic product, and agricultural value added (AVA) contribute to the food security supply. In contrast, urbanization's negative but insignificant contribution to the food supply in selected economies exists. Besides, another core objective of the study is to investigate the moderate role of the energy transition on the gross domestic product, agriculture sector, and land use and find the significant contribution to the food supply. However, the current study also tries forecasting for the next 10 years and employs the impulse response function (IRF) and variance decomposition analysis (VDA). Congruently, this study uses the pairwise panel causality test and finds exciting outcomes. The COVID-19 crisis has posed challenges such as energy consumption and food security issues. On behalf of the results, the current study proposes imperative policies to investigate the desired level of food supply. The findings provide valuable insights for experts, policymakers, and officials to take practical measures for energy use and food security challenges.
Temporal and sequence-related variability in diffusion-weighted imaging of presumed cerebrovascular accidents in the dog brain
Authors: Boudreau Elizabeth , Kerwin Sharon C. , DuPont Emily B. , Levine Jonathan M. , Griffin John F.
Published: November 2022

Diffusion-weighted MRI (DWI) is often used to guide clinical interpretation of intraparenchymal brain lesions when there is suspicion for a cerebrovascular accident (CVA). Despite widespread evidence that imaging and patient parameters can influence diffusion-weighted measurements, such as apparent diffusion coefficient (ADC), there is little published data on such measurements for naturally occurring CVA in clinical cases in dogs. We describe a series of 22 presumed and confirmed spontaneous canine CVA with known time of clinical onset imaged on a single 3T magnet between 2011 and 2021. Median ADC values of < 1.0x10−3 mm2/s were seen in normal control tissues as well as within CVAs. Absolute and relative ADC values in CVAs were well-correlated (R2 = 0.82). Absolute ADC values < 1.0x10−3 mm2/s prevailed within ischemic CVAs, though there were exceptions, including some lesions of < 5 days age. Some lesions showed reduced absolute but not relative ADC values when compared to matched normal contralateral tissue. CVAs with large hemorrhagic components did not show restricted diffusion. Variation in the DWI sequence used impacted the ADC values obtained. Failure to identify a region of ADC < 1.0x10−3 mm2/s should not exclude CVA from the differential list when clinical suspicion is high.
A Phi29-based unbiased exponential amplification and genotyping approach improves pathogen detection in tick samples
Authors: Zhang Xi , Chen Jingjing , Jiang Pengfei , Xu Heling , Zhang Qi , Zhang Huan , Han Xiaohu , Chen Zeliang
Published: November 2022

Ticks are vectors for many infectious diseases, such as spotted fever group (SFG) rickettsioses and borreliosis, and are valuable in the study of pathogen ecology. Ticks have several growth stages that vary considerably in size; therefore, in most cases, DNA extracted from ticks is insufficient for subsequent studies, particularly for multiple pathogen screening and genotyping. Unbiased amplification of DNA from tick samples before analysis is a major requirement for subsequent ecological surveys and other studies. Phi29 DNA polymerase, an enzyme that exhibits strand displacement activity, can exponentially amplify DNA randomly, generating large quantities of DNA. In the present study, we developed a Phi29-based unbiased exponential amplification (PEA) assay to obtain sufficient tick DNA for genetic analysis. By using tick-borne pathogen detection and genotyping as a model, we tested and evaluated the feasibility of the assay. DNA was extracted from single ticks and subjected to PEA. The results showed that tick DNA could be amplified up to 105 fold. The amplified products were successfully used for pathogen screening and genotyping. Rickettsia was successfully detected and genotyped in samples with amplified DNA from single ticks. Furthermore, we identified a new genotype of Rickettsia from ticks collected from Dandong city, Liaoning province, Northeast China. This PEA assay is universal and can be extended to other applications where the quantity of DNA is greatly limited.
Multi-Disturbance Observers-Based Nonlinear Control Scheme for Wire Rope Tension Control of Hoisting Systems with Backstepping
Authors: Zang Wanshun , Chen Xiao , Zhao Jun
Published: November 2022

The objective of this paper is to pursue a wire rope control methodology for reducing the tension difference between two wire ropes of a hoisting system. As we know, complicated disturbances exist in the complex electro-hydraulic hoisting system, notably, some of these disturbances are coupled, such as high-speed airflow disturbances, structure vibrations and vibrations in flexible wire ropes. Furthermore, there are model errors in force modeling due to the Coulomb friction between two wire ropes and two moveable head sheaves in the real physical hoisting systems. To eliminate disturbances, two types of disturbance observers (DOs) are employed: a traditional disturbance observer (TDO) and a coupled disturbance observer (CDO), both of which are utilized to estimate and compensate for the Coulomb friction and coupled disturbances online. As a result, a nonlinear backstepping control scheme is presented with estimation values from the TDO and the CDO. The experiment’s results demonstrate the effectiveness of the proposed control methodology.
Mapping of Soil pH Based on SVM-RFE Feature Selection Algorithm
Authors: Guo Jia , Wang Ku , Jin Shaofei
Published: November 2022

The explicit mapping of spatial soil pH is beneficial to evaluate the effects of land-use changes in soil quality. Digital soil mapping methods based on machine learning have been considered one effective way to predict the spatial distribution of soil parameters. However, selecting optimal environmental variables with an appropriate feature selection method is key work in digital mapping. In this study, we evaluated the performance of the support vector machine recursive feature elimination (SVM-RFE) feature selection methods with four common performance machine learning methods in predicting and mapping the spatial soil pH of one urban area in Fuzhou, China. Thirty environmental variables were collected from the 134 samples that covered the entire study area for the SVM-RFE feature selection. The results identified the five most critical environmental variables for soil pH value: mean annual temperature (MAT), slope, Topographic Wetness Index (TWI), modified soil-adjusted vegetation index (MSAVI), and Band5. Further, the SVM-RFE feature selection algorithm could effectively improve the model accuracy, and the extreme gradient boosting (XGBoost) model after SVM-RFE feature selection had the best prediction results (R2 = 0.68, MAE = 0.16, RMSE = 0.26). This paper combines the RFE-SVM feature selection with machine learning models to enable the fast and inexpensive mapping of soil pH, providing new ideas for predicting soil pH at small and medium scales, which will help with soil conservation and management in the region.
Meeting the Challenges Facing Wheat Production: The Strategic Research Agenda of the Global Wheat Initiative
Authors: Langridge Peter , Alaux Michael , Almeida Nuno Felipe , Ammar Karim , Baum Michael , Bekkaoui Faouzi , Bentley Alison R. , Beres Brian L. , Berger Bettina , Braun Hans-Joachim , Brown-Guedira Gina , Burt Christopher James , Caccamo Mario Jose , Cattivelli Luigi , Charmet Gilles , Civáň Peter , Cloutier Sylvie , Cohan Jean-Pierre , Devaux Pierre J. , Doohan Fiona M. , Dreccer M. Fernanda , Ferrahi Moha , Germán Silvia E. , Goodwin Stephen B. , Griffiths Simon , Guzmán Carlos , Handa Hirokazu , Hawkesford Malcolm John , He Zhonghu , Huttner Eric , Ikeda Tatsuya M. , Kilian Benjamin , King Ian Philip , King Julie , Kirkegaard John A. , Lage Jacob , Le Gouis Jacques , Mondal Suchismita , Mullins Ewen , Ordon Frank , Ortiz-Monasterio Jose Ivan , Özkan Hakan , Öztürk İrfan , Pereyra Silvia A. , Pozniak Curtis J. , Quesneville Hadi , Quincke Martín C. , Rebetzke Greg John , Christoph Reif Jochen , Saavedra-Bravo Teresa , Schurr Ulrich , Sharma Shivali , Singh Sanjay Kumar , Singh Ravi P. , Snape John W. , Tadesse Wuletaw , Tsujimoto Hisashi , Tuberosa Roberto , Willis Tim G. , Zhang Xueyong
Published: November 2022

Wheat occupies a special role in global food security since, in addition to providing 20% of our carbohydrates and protein, almost 25% of the global production is traded internationally. The importance of wheat for food security was recognised by the Chief Agricultural Scientists of the G20 group of countries when they endorsed the establishment of the Wheat Initiative in 2011. The Wheat Initiative was tasked with supporting the wheat research community by facilitating collaboration, information and resource sharing and helping to build the capacity to address challenges facing production in an increasingly variable environment. Many countries invest in wheat research. Innovations in wheat breeding and agronomy have delivered enormous gains over the past few decades, with the average global yield increasing from just over 1 tonne per hectare in the early 1960s to around 3.5 tonnes in the past decade. These gains are threatened by climate change, the rapidly rising financial and environmental costs of fertilizer, and pesticides, combined with declines in water availability for irrigation in many regions. The international wheat research community has worked to identify major opportunities to help ensure that global wheat production can meet demand. The outcomes of these discussions are presented in this paper.
The Upregulation of Cathepsin G Is Associated with Resistance to Bovine Paratuberculosis
Authors: Canive Maria , Badia-Bringué Gerard , Alonso-Hearn Marta
Published: November 2022

An in silico genomic–transcriptomic combined approach allowed the identification of a polymorphism (cis-eQTL-rs41976219) in the Bos taurus genome associated with the CTSG mRNA expression in bovine blood samples, which suggests that individual genetic variation might modulate the CTSG transcriptional response. In the current study, a sandwich ELISA is used to measure the CTSG protein levels in supernatants of monocyte-derived macrophages (MDMs) isolated from cows with the AA (n = 5) and AC (n = 11) genotypes for the rs41976219 and infected ex vivo with MAP. Cows with the AC genotype have significantly higher CTSG protein levels (1.85 ng/mL) in the supernatants of enriched CD14+-MDMs after 2 h of infection when compared with infected CD14+-MDMs from cows with the AA genotype (1.68 ng/mL). Statistically significant differences in the intracellular MAP load at 7 d p.i. are observed between animals with the AA (2.16 log CFUs) and AC (1.44 log CFUs) genotypes. Finally, the association between the rs41976219 allelic variants and resistance to PTB is tested in a larger cattle population (n = 943) classified according to the presence (n = 442) or absence (n = 501) of PTB-associated lesions. The presence of the two minor alleles in the rs41976219 (CC) is more frequent among healthy cows than in cows with PTB-associated lesions in gut tissues (2.2% vs. 1.4%, OR = 0.61). In agreement with this, the CTSG levels in plasma samples of cows without lesions in gut tissues and with the CC (n = 8) genotype are significantly higher than in the plasmas of cows with the AA + AC (n = 36) genotypes.
Xylanase Impact beyond Performance: Effects on Gut Structure, Faecal Volatile Fatty Acid Content and Ammonia Emissions in Weaned Piglets Fed Diets Containing Fibrous Ingredients
Authors: Boontiam Waewaree , Phaenghairee Pheeraphong , Van Hoeck Veerle , Vasanthakumari Bindhu Lakshmibai , Somers Ingrid , Wealleans Alexandra
Published: November 2022

The addition of xylanase to piglet diets is known to improve performance and nutrient digestibility. The present study aimed to assess the impact of new xylanase on the growth performance, nutrient digestibility, and gut function of weaned piglets. A total of 144 pigs, weaned at 28 days (7.48 kg initial body weight, IBW), were assigned to 36 pens and 9 pens per treatment. Dietary treatments were a basal complex control diet, and the basal diet supplemented with 45,000, 90,000 and 135,000 U/kg xylanase. Performance was measured at days 0, 14 and 35. At day 35, samples were collected for assessment of intestinal histology, and volatile fatty acid and ammonia concentrations. After two weeks post-weaning, additional 12 piglets (11.34 kg IBW) were placed in metabolic crates for assessment of apparent total tract nutrient digestibility using a dietary marker. The addition of xylanase at 90,000 and 135,000 U/kg significantly improved average daily gain (333.6 g/day control, 364.86 g/day, 90,000 U/kg, 405.89 g/day, 135,000 U/kg, p < 0.05), G:F (0.557 control, 0.612 90,000 U/kg, 0.692 135,000 U/kg, p < 0.05), and reduced diarrhoea. This was driven improved nutrient digestibility and villus height in the jejunum (372.87 µm control, 432.53 µm 45,000 U/kg, 465.80 µm 90,000 U/kg, 491.28 µm 135,000 U/kg, p < 0.05). Xylanase supplementation also linearly increased faecal butyrate levels and had a quadratic relationship with propionate concentrations. 135,000 U/kg xylanase also reduced ammonia emissions. In conclusion, dietary supplementation with xylanase improved growth performance and feed efficiency in weaning piglets, likely driven by improvements to gut structure and function.
L-Tryptophan Differentially Regulated Glucose and Amino Acid Transporters in the Small Intestine of Rat Challenged with Lipopolysaccharide
Authors: Wang Bin , Jiang Lili , Wu Zhenlong , Dai Zhaolai
Published: November 2022

Tryptophan (Trp) has been shown to improve the growth and gut function of weaned piglets. Whether the growth-promoting effect of Trp is due to the improvement in nutrient transport and absorption during weaning or under conditions of inflammation has not been fully characterized. The objective of this study was to determine the effects of Trp on lipopolysaccharide (LPS)-induced changes in glucose and amino acid (AA) transport in the rat jejunum. Twenty-four 7-week-old Sprague Dawley rats were randomly divided into one of three groups: control, LPS, and Trp + LPS. Rats were supplemented with 0 or 0.1 mg Trp per gram body weight/d in drinking water for 7 days and were intraperitoneally injected with LPS (5 mg/kg BW) on day 8. After 24 h, rats were sacrificed, and jejunum samples were isolated for the analysis of glucose and AA transport using an Ussing chamber and the expression of glucose and AA transporters. The results showed that Trp alleviated the LPS-induced increase in jejunal permeability (p < 0.05) and decrease in changes in the short-circuit current of glucose, arginine, glutamine, glutamate, glycine, histidine, leucine, lysine, taurine, threonine, and Trp (p < 0.05). Trp reversed (p < 0.05) the LPS-induced downregulation of expression of the glucose transporter SGLT1 and AA transporters solute carrier family 38 member 2 (SNAT2) and solute carrier family 7 member 8 (LAT2), as well as ATPase Na+/K+ transporting subunit alpha 2 (ATP1A2). However, Trp increased (p < 0.01) the LPS-induced upregulation of acidic AA transporter solute carrier family 1 member 1 (EAAT3) expression. The above findings may help to develop nutritional interventions for the differential targeting of gut nutrient transporters, aiming to improve gut function and health in the presence of inflammation in both humans and animals.
Equine Stomach Development in the Foetal Period of Prenatal Life—A Histological and Histometric Study
Authors: Poradowski Dominik , Chrószcz Aleksander
Published: November 2022

Histological and morphometrical analysis of the stomach wall was performed during the foetal period divided into three age groups (4th–11th month of gestation). The material was taken from non-glandular (the blind ventricular sac) and glandular parts (the plicated edge margin/cardiac part, the body of stomach and the pyloric part) of the stomach. It was preserved and prepared according to the standard protocol. The histological slides were stained (H-E, Masson-Goldner and PAS). The analyses were performed using the light microscope. All measurements were statistically elaborated. The crown-rump length growth rate was estimated as isometric. The blind ventricular sac growth rate was lower than CRL (negative allometric) and the partition of stomach mucosa into non-glandular and glandular part occurred in the 1st age group. The plicated edge margin/cardiac part and the pyloric part shoved similar tendencies. Only the body of stomach demonstrated a higher growth rate than CRL (positive allometric), which can be explained due to the strongest development of fundic glands. Moreover, comparing the adult reference group to the three parts of the foetal period, all metric values were lower than those achieved prenatally. The blind ventricular sac was covered with the multiple plane epithelium. The glandular parts of stomach that formed the superficial concave areas were covered with the simple columnar epithelium in the 1st age group, which developed to the cardiac, fundic, and pyloric glands in the 2rd and 3rd age groups. The propria mucosae was built with the mesenchyme, which differentiated later to the loose connective tissue. The muscular layer of mucosa was not clearly distinguishable in the 1st age group. The muscular layer of the stomach wall was formed with myoblasts in the 1st age group and later in the 2nd and the 3rd age groups built with fusiform myocytes divided into internal and external layers. The non-differentiated cells of glandular epithelium transformed into the parietal and chief cells. The first were visible in the gastric glands of the 2nd age group. Both of them were present in the 3rd age group gastric mucosa. The PAS staining proved a moderate PAS-positive reaction in the 2rd age group, while it was estimated as intense Pas-positive in the gastric glands in the 3rd age group and was comparable to postnatal observation (the adult reference group).
Recycling of Citric Acid Waste for Potential Use as Animal Feed through Fermentation with Lactic Acid Bacteria and a Mixture of Fibrolytic Enzymes
Authors: Tanpong Sirisak , Wongtangtintharn Sawitree , Cherdthong Anusorn , Prachumchai Rittikeard , Tengjaroenkul Bundit , Chanjula Pin , Suntara Chanon , Wachirapakorn Chalong
Published: November 2022

Once improperly managed, the citric acid production industry generates waste, which contributes to pollution and other environmental issues. We proposed that, with sufficient quality improvement, citric acid by-product (CAP) might be used for animal feed, thereby reducing the environmental impact. The aim of the present study was to ferment citric acid by-product (CAP) by inoculation with lactic acid bacteria (LAB) and a fibrolytic enzyme mixture for quality improvement and crude fiber reduction in the waste products. LAB inoculants were L. casei TH14, and the additive enzyme used was a fibrolytic enzyme mixture (glucanase, pectinase, and carboxymethylcellulase) of a small-scale fermentation method. The seven treatments employed in this study were as follows: (1) control (untreated), (2) CAP-inoculated L. casei TH14 at 0.01% DM, (3) CAP-inoculated L. casei TH14 at 0.05% DM, (4) CAP-inoculated enzymes at 0.01% DM, (5) CAP-inoculated enzymes at 0.05% DM, (6) CAP-inoculated L. casei TH14 at 0.01% DM with enzymes at 0.01% DM, and (7) CAP-inoculated L. casei TH14 at 0.05% DM with enzymes at 0.05% DM. The samples were taken on days 1, 7, 14, 21, and 28 of ensiling, both before and after. Four replications were used. The results of the chemical composition of the CAP before and after ensilage inoculated with L. casei TH14 did not show any differences in crude protein, ether extract, ash, or gross energy, but the enzymes significantly (p < 0.05) decreased crude fiber and increased nitrogen-free extract. The combination was especially effective at improving the characteristics of CAP, with a reduction in crude fiber from 21.98% to 22.69%, of neutral detergent fiber (NDF) from 16.01% to 17.54%, and of acid detergent fiber (ADF) from 13.75% to 16.19%. Furthermore, the combination of L. casei TH14 and the enzyme increased crude protein from 1.75% to 2.24% at 28 days of ensiling. Therefore, CAP-inoculated L. casei TH14 did not change in chemical composition, while crude fiber, NDF, and ADF decreased when CAP was inoculated with enzyme. The combination of L. casei TH14 and the enzyme is more effective at improving chemical composition and reducing crude fiber and enhancing carbohydrate breakdown in the CAP. Finally, by enhancing the CAP’s quality, it may be possible to use it in animal feed and minimize its impact on the environment.
The Analysis of E-Cadherin, N-Cadherin, Vimentin, HER-2, CEA, CA15-3 and SF Expression in the Diagnosis of Canine Mammary Tumors
Authors: Yu Chao , Zheng Huihua , Liu Xiangyu , Xie Guanghong
Published: November 2022

Canine mammary tumors (CMTs) are one of the most common tumors in female dogs, and they are associated with a poor prognosis owing to their high rate of recurrence and metastasis rates, which make their diagnosis especially important in clinical veterinary medicine. In this study, the characteristics of tumors were observed in dogs suffering from CMTs, and clinical diagnosis and histopathology were used to identify tumors. Furthermore, the expression levels of tumor markers for CMTs were analyzed by enzyme-linked immunosorbent assay (ELISA) and quantitative PCR (qPCR). Upon clinical examination, dogs with CMTs displayed a distinct and irregular mass in the mammary gland, and X-ray (Latero-lateral and ventro-dorsal views) and ultrasonography of the abdomen revealed a moderately echogenic mass at the mammary gland with slightly stronger density than the surrounding tissue. A total of 30 tumors were identified by histopathology, 11 benign and 19 malignant. Changes in some indicators in dogs suffering from CMTs and healthy dogs suggested that there were multiple direct or paraneoplastic changes associated with tumors that could be detected/suspected by hematological examination, and ELISA revealed the HER-2 serum concentrations were significantly different between healthy animals and those with malignant tumors. qPCR indicated that tumor markers N-cadherin, Vimentin, HER-2, CEA, CA15-3 and SF were higher in dogs with malignant tumors than healthy dogs, with a low level of E-cadherin in malignant tumors. This study verified that serological tests and molecular biological tests were essential to the early diagnosis, treatment and prognosis of dogs with tumors.
Pre-Visit Use of Non-Prescribed Antibiotics among Child Patients in China: Prevalence, Predictors, and Association with Physicians’ Prescribing of Antibiotics at Medical Visits
Authors: Wang Nan Christine
Published: November 2022

Antibiotic resistance is one of the most serious global public health crises. Inappropriate use of antibiotics is an important contributor. Using a cross-sectional survey, we recruited 3056 caregivers from 21 provinces in China to complete a questionnaire pertaining to their most recent medical visits for children’s acute respiratory tract infection (ARTI) symptoms in October 2013. The findings show that the prevalence of caregivers giving children non-prescribed antibiotics before their medical visit was as high as 38%. Caring for an older child and having lower educational attainment increased the likelihood of self-medication with antibiotics; among Chinese residential areas, caregivers living in tier 2 urban districts were most likely to administer non-prescribed antibiotics before their visit. Physicians’ prescribing of antibiotics was significantly associated with caregivers’ self-reported pre-visit use of non-prescribed antibiotics. Misuse should be addressed by regulating the sale of antibiotics and improving communication at medical consultations.
The Potential of Phenothiazines against Endodontic Pathogens: A Focus on Enterococcus-Candida Dual-Species Biofilm
Authors: Fiallos Nicole de Mello , Ribeiro Aguiar Ana Luiza , da Silva Bruno Nascimento , Pergentino Mariana Lara Mendes , Rocha Marcos Fábio Gadelha , Sidrim José Júlio Costa , Maia Débora Castelo Branco de Souza Collares , Cordeiro Rossana de Aguiar
Published: November 2022

Persistent apical periodontitis occurs when the endodontic treatment fails to eradicate the intraradicular infection, and is mainly caused by Gram-positive bacteria and yeasts, such as Enterococcus faecalis and Candida albicans, respectively. Phenothiazines have been described as potential antimicrobials against bacteria and fungi. This study aimed to investigate the antimicrobial potential of promethazine (PMZ) and chlorpromazine (CPZ) against E. faecalis and C. albicans dual-species biofilms. The susceptibility of planktonic cells to phenothiazines, chlorhexidine (CHX) and sodium hypochlorite (NaOCl) was initially analyzed by broth microdilution. Interaction between phenothiazines and CHX was examined by chequerboard assay. The effect of NaOCl, PMZ, CPZ, CHX, PMZ + CHX, and CPZ + CHX on biofilms was investigated by susceptibility assays, biochemical and morphological analyses. Results were evaluated through one-way ANOVA and Tukey’s multiple comparison post-test. PMZ, alone or in combination with irrigants, was the most efficient phenothiazine, capable of reducing cell counts, biomass, biovolume, carbohydrate and protein contents of dual-species biofilms. Neither PMZ nor CPZ increased the antimicrobial activity of CHX. Further investigations of the properties of phenothiazines should be performed to encourage their use in endodontic clinical practice.
Development of a New Colorimetric, Kinetic and Automated Ceruloplasmin Ferroxidase Activity Measurement Method
Authors: Neşelioğlu Salim , Fırat Oğuz Esra , Erel Özcan
Published: November 2022

Background: Ceruloplasmin plays an important role in the regulation of iron metabolism. Ceruloplasmin is an acute-phase protein known to have many metabolic effects. Its activity increases during infection, inflammation, and compensation of oxidation. In the current study, our aim is to develop a new method for the measurement of ferroxidase activity without requiring any chromogen. Methods: Venous blood samples were collected into serum separator tubes. Ferric iron ions formed by the enzyme ferroxidase were measured, both manually and fully automatically, at the 415 nm wavelength without using chromogen. These results were compared to conventional ferroxidase measurement methods and to the immunoturbidimetric ceruloplasmin measurement method. Results: The detection limit of the new assay was 14.8 U/L. The upper limit of the linearity was 1380 U/L. Precision values were calculated for high, medium, and low levels of ferroxidase activity in serum pool. The coefficient of variation was <5% for each level. Conclusion: In the present method, chromogens are not used. With its considerably low cost and short reaction time, this method is able to provide fast results, can be performed easily, and makes accurate measurements.
Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis
Authors: Conceição Mariana , Gushiken Lucas Fernando Sérgio , Aldana-Mejía Jennyfer Andrea , Tanimoto Matheus Hikaru , Ferreira Marcos Vital de Sá , Alves Andreia Cristina Miranda , Miyashita Marina Naomi , Bastos Jairo Kenupp , Beserra Fernando Pereira , Pellizzon Cláudia Helena
Published: November 2022

Skin wound healing is a complex process that requires the mutual work of cellular and molecular agents to promote tissue restoration. In order to improve such a process, especially in cases of impaired healing (e.g., diabetic ulcer, chronic wounds), there is a search for substances with healing properties and low toxicity: two features that some natural products—such as the bee product named propolis—exhibit. Propolis is a resinous substance obtained from plant resins and exudates with antioxidant, anti-inflammatory, and antitumoral activities, among other biological ones. Based on the previously reported healing actions of different types of propolis, the Brazilian red propolis (BRP) was tested for this matter. A skin wound excision model in male Wistar rats was performed using two topical formulations with 1% red propolis as treatments: hydroalcoholic extract and Paste. Macroscopical, histological and immunohistochemical analysis were performed, revealing that red propolis enhanced wound contraction, epithelialization, reduced crust formation, and modulated the distribution of healing associated factors, mainly collagen I, collagen III, MMP-9, TGF-β3 and VEGF. Biochemical analysis with the antioxidants SOD, MPO, GSH and GR showed that propolis acts similarly to the positive control, collagenase, increasing these molecules’ activity. These results suggest that BRP promotes enhanced wound healing by modulating growth factors and antioxidant molecules related to cutaneous wound healing.
Targeting Ferroptosis as a Promising Therapeutic Strategy for Ischemia-Reperfusion Injury
Authors: Pan Yihang , Wang Xueke , Liu Xiwang , Shen Lihua , Chen Qixing , Shu Qiang
Published: November 2022

Ischemia-reperfusion (I/R) injury is a major challenge in perioperative medicine that contributes to pathological damage in various conditions, including ischemic stroke, myocardial infarction, acute lung injury, liver transplantation, acute kidney injury and hemorrhagic shock. I/R damage is often irreversible, and current treatments for I/R injury are limited. Ferroptosis, a type of regulated cell death characterized by the iron-dependent accumulation of lipid hydroperoxides, has been implicated in multiple diseases, including I/R injury. Emerging evidence suggests that ferroptosis can serve as a therapeutic target to alleviate I/R injury, and pharmacological strategies targeting ferroptosis have been developed in I/R models. Here, we systematically summarize recent advances in research on ferroptosis in I/R injury and provide a comprehensive analysis of ferroptosis-regulated genes investigated in the context of I/R, as well as the therapeutic applications of ferroptosis regulators, to provide insights into developing therapeutic strategies for this devastating disease.
Air Quality Improvement in China: Evidence from PM2.5 Concentrations in Five Urban Agglomerations, 2000–2021
Authors: Zhao Chuanwu , Pan Yaozhong , Teng Yongjia , Baqa Muhammad Fahad , Guo Wei
Published: November 2022

Air pollution endangers human health and sustainable socio-economic development, especially in urban agglomeration (UA). The Chinese government has implemented a series of policies and standards to improve air quality. However, few studies have compared variations in PM2.5 concentrations across multiple UAs, and current research often lacks analysis relative to the clean air policies implemented by the government. In this study, we used econometric and geostatistical methods to assess the distribution and spatial evolution of PM2.5 concentrations in five UAs (the Beijing–Tianjin–Hebei UA (BTHUA), middle reaches of the Yangtze River UA (MYRUA), Chengdu–Chongqing UA (CCUA), Harbin Changchun UA (HCUA), and Beibu Gulf UA (BGUA)) in China from 2000 to 2021 to explore the effectiveness of the clean air policies implemented by the government on air pollution control, to analyze the ambient air quality of UAs, and to make recommendations for public outdoor activities. The results indicated that the clean air policy implemented by the Chinese government in 2013 achieved significant treatment results. PM2.5 concentrations were plotted as an inverted U-shaped curve based on time, which showed an upward trend before 2013 and a downward trend after 2013. PM2.5 concentrations showed a similar seasonal pattern, with a single-valley “V” shape. PM2.5 concentration was the highest in winter and the lowest in summer. The PM2.5 concentration of HCUA and BGUA was lower than that of CCUA, MYRUA, and BTHUA. The increase in PM2.5 concentration mainly occurred in autumn and winter, while the decrease mainly occurred in spring. In 2021, the PM2.5 air quality compliance rates (<35 µg/m3) in BTHUA, MYRUA, CCUA, HCUA, and BGUA were 44.57%, 80.00%, 82.04%, 99.74%, and 100%, respectively. However, in 2021, 19.19% of the five UAs still had an ambient air quality of Grade II (i.e., 50 < AQIPM2.5 < 100). People with abnormally sensitive breathing in these areas should reduce their outdoor activities. These results contribute to epidemiological studies on human health and disease prevention and suggest reasonable pathways by which governments can improve air quality through sustainable urban planning.
Spatial Homogenization Adjustment and Application of Weather Station Networks in Xinjiang, China
Authors: Zhang Liancheng , Si Jiayi , Jiapaer Guli , Zhang Taixi , Mao Weiyi , Dong Siyan
Published: November 2022

In this study, we define the S0 value (buffer zone area centred on a meteorological station) and two inhomogeneity measurement parameters, the station domain area and station network density, for 89 weather stations in the Xinjiang region, and we construct the weight coefficient of the station network according to the station domain area. Applying the weight coefficient, we calculate the mean temperature, maximum temperature, and minimum temperature in January, April, July, October, and annually in the Xinjiang region from 1961 to 2021. The results show that the S0 value of 200,000 km2 is suitable for determining the weight coefficient of the station network in the Xinjiang region. The two measurement parameters can quantitatively reflect the inhomogeneity of the distribution of 89 weather stations in the Xinjiang region. The spatial distribution density of the station network is positively proportional to the station network density and inversely proportional to the station domain area and weight coefficient of the stations. The equal-weighted average is lower than the spatially homogenized revised average, which underestimates the mean temperature in the Xinjiang region, and the spatially homogenized revised average better reflects the real temperature in the Xinjiang region. The annual and monthly mean temperatures, maximum and minimum temperatures calculated by the spatially homogenized revised average, and the equal-weighted average have the same upwards trend, and the mean temperature warming trend calculated by the two methods have differences, but the differences are not significant. The annual, January, April, July, and October minimum temperature warming trends according to the spatial homogenization revised average are greater than the maximum temperature warming trend and the mean temperature warming trend, and the annual minimum temperature warming trend is 3.3 times the annual maximum warming trend and two times the annual mean temperature.