bluuebunny/crossref_metadata_2025 huggingface dataset:
The molecular characteristics of spinal cord gliomas with or without H3 K27M mutation
Authors: Chai Rui-Chao , Zhang Yao-Wu , Liu Yu-Qing , Chang Yu-Zhou , Pang Bo , Jiang Tao , Jia Wen-Qing , Wang Yong-Zhi
Published: March 2020

Due to the rare incidence of spinal cord astrocytomas, their molecular features remain unclear. Here, we characterized the landscapes of mutations in H3 K27M, isocitrate dehydrogenase 1 (IDH1) R132H, BRAF V600E, and the TERT promoter in 83 diffuse spinal cord astrocytic tumors. Among these samples, thirty-five patients had the H3 K27M mutation; this mutant could be observed in histological grade II (40%), III (40%), and IV (20%) astrocytomas. IDH1 mutations were absent in 58 of 58 cases tested. The BRAF V600E mutation (7/57) was only observed in H3-wildtype astrocytomas, and was associated with a better prognosis in all histological grade II/III astrocytomas. TERT promoter mutations were observed in both H3 K27M-mutant (4/25) and -wildtype (9/33) astrocytomas, and were associated with a poor prognosis in H3-wildtype histological grade II/III astrocytomas. In the 2016 WHO classification of CNS tumors, H3 K27M-mutant diffuse midline gliomas, including spinal cord astrocytomas, are categorized as WHO grade IV. Here, we noticed that the median overall survival of histological grade II/III H3 K27M-mutant cases (n = 28) was significantly longer than that of either the total histological grade IV cases (n = 12) or the H3 K27M-mutant histological grade IV cases (n = 7). We also directly compared H3 K27M-mutant astrocytomas to H3-wildtype astrocytomas of the same histological grade. In histological grade II astrocytomas, compared to H3-wildtype cases (n = 37), H3 K27M-mutant patients (n = 14) had showed a significantly higher Ki-67-positive rate and poorer survival rate. However, no significant differences in these parameters were observed in histological grade III and IV astrocytoma patients. In conclusion, these findings indicate that spinal cord astrocytomas are considerably different from hemispheric and brainstem astrocytomas in terms of their molecular profiles, and that the histological grade cannot be ignored when assessing the prognosis of H3 K27M-mutant spinal cord astrocytomas.
Quantitative analysis of mixed pigments for Chinese paintings using the improved method of ratio spectra derivative spectrophotometry based on mode
Authors: Lyu Shuqiang , Liu Yiyi , Hou Miaole , Yin Qinli , Wu Wangting , Yang Xueyun
Published: March 2020

Background

Mixed pigment analysis is an important and complex subject in preserving and restoring Chinese paintings since the colors observed by our naked eyes or instruments such as hyperspectral cameras are usually a mixture of several kinds of pigments. The purpose of this study was to explore a more effective method to confirm the type of every pure pigment and their proportion in pigment mixtures on the surface of paintings.

Methods

Two endmember extraction algorithms were adopted to identify the types of pigments and an improved method of ratio spectra derivative spectrophotometry was used to determine their proportion.

Main works

(1) Extracting the pure pigment components from mixed spectrum by adopting two blind source separation algorithms: Independent Component Analysis and Non-negative Matrix Factorization; (2) matching the separated pure spectrum with the pigment spectral library built in our laboratory to determine the pigment type; and (3) calculating the proportions of mixed pigments using the Ratio Spectra Derivative Spectrophotometry based on Mode, which is improved based on the original algorithm. Finally, a comparison was made with two abundance inversion algorithms: Least Squares Algorithm and Minimum Volume Simplex Analysis. The correlation coefficient and root mean square error were used to provide evidence for accuracy evaluation.

Conclusions

(1) Non-negative matrix factorization is more suitable for endmember extraction; and (2) Ratio spectra derivative spectrophotometry based on mode is more suitable for abundance inversion.
A Randomised, Placebo-Controlled, Crossover Study Investigating the Optimal Timing of a Caffeine-Containing Supplement for Exercise Performance
Authors: Davenport Andrew D. , Jameson Tom S. O. , Kilroe Sean P. , Monteyne Alistair J. , Pavis George F. , Wall Benjamin T. , Dirks Marlou L. , Alamdari Nima , Mikus Catherine R. , Stephens Francis B.
Published: March 2020

Background

Pre-exercise supplements containing low doses of caffeine improve endurance exercise performance, but the most efficacious time for consumption before intense endurance exercise remains unclear, as does the contribution of caffeine metabolism.

Methods

This study assessed the timing of a commercially available supplement containing 200 mg of caffeine, 1600 mg of β-alanine and 1000 mg of quercetin [Beachbody Performance Energize, Beachbody LLC, USA] on exercise performance, perception of effort and plasma caffeine metabolites. Thirteen cyclists (V̇O2max 64.5 ± 1.4 ml kg− 1 min− 1 (± SEM)) completed four experimental visits consisting of 30 min of steady-state exercise on a cycle ergometer at 83 ± 1% V̇O2max followed by a 15-min time trial, with perceived exertion measured regularly. On three of the visits, participants consumed caffeine either 35 min before steady-state exercise (PRE), at the onset of steady-state (ONS) or immediately before the time trial (DUR) phases, with a placebo consumed at the other two time points (i.e. three drinks per visit). The other visit (PLA) consisted of consuming the placebo supplement at all three time points. The placebo was taste-, colour- and calorie-matched.

Results

Total work performed during the time trial in PRE was 5% greater than PLA (3.53 ± 0.14 vs. 3.36 ± 0.13 kJ kg− 1 body mass; P = 0.0025), but not ONS (3.44 ± 0.13 kJ kg− 1; P = 0.3619) or DUR (3.39 ± 0.13 kJ kg− 1; P = 0.925), which were similar to PLA. Perceived exertion was lowest during steady-state exercise in the PRE condition (P < 0.05), which coincided with elevated plasma paraxanthine in PRE only (P < 0.05).

Conclusion

In summary, ingestion of a pre-exercise supplement containing 200 mg caffeine 35 min before exercise appeared optimal for improved performance in a subsequent fatiguing time trial, possibly by reducing the perception of effort. Whether this was due to increased circulating paraxanthine requires further investigation.

Trial registration

ClinicalTrials.Gov,NCT02985606; 10/26/2016.
Exploration of indigenous strains of the green muscardine fungus from soils and their pathogenicity against the tobacco caterpillar, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae)
Authors: Dhanapal R. , Kumar D. V. Sai Ram , Lakshmipathy R. , Rani C. Sandhya , Kumar V. Manoj
Published: March 2020

In the recent years, the keen interest has been increased towards biological control-based management practices using entomopathogenic fungi (EPF) for the control of many economically important insects. In this management practices, selection of right strains of EPF is an important criteria to control the target pests in effective manner. In this context, this research work was formulated to find out environmentally effective strains of EPF in the soils of costal districts of Andhra Pradesh. Out of 135 soil samples collected, 8 soil samples yielded 8 strains of Metarhizium anisopliae. Among all 8 M. anisopliae isolates, the least median lethal concentration (LC50) for 2nd instar of the tobacco caterpillar, Spodoptera litura (Lepidoptera: Noctuidae) was 1.24 × 105 spores ml-1 in M-7, followed by 1.72 × 105 spores ml-1 in M-5 and 1.86 × 105 spores ml-1 in M-4 in an increasing order. Likewise, the median lethal time (LT50) values of different isolates of EPF against 2nd instar of S.litura were calculated for a uniform highest dose of 1 × 108 spores ml−1. Among all 8 M. anisopliae isolates, the lowest LT50 was observed as 186.44 hours post infection (hpi) with M-5 and 189.34 hpi with M-7.
Estimation method of SBAS dual-frequency range error integrity parameter
Authors: Shao Bo , Ding Qun , Wu Xianbing
Published: March 2020

The development of a dual-frequency multi-constellation satellite-based augmentation system (DFMC SBAS) is in progress worldwide. The broadcasted dual-frequency range error (DFRE) integrity parameter reflects the effects of satellite ephemeris and clock corrections. A user uses the DFRE to calculate the protection level and then determines whether the DFMC SBAS service satisfies the requirements of the current flight phase. However, the calculation of the DFRE has not been reported. Herein, a DFRE estimation method is proposed based on the projection method. Using the ephemeris–clock covariance matrix of each satellite, the maximal projection direction was solved, and the projection of the covariance matrix on this direction was used as the DFRE to form an envelope for the maximal corrected error. Results show that the DFRE can form an envelope of the maximal corrected error with a set probability, and the integrity performance in the user segment satisfies the Category I precision approach requirement.
Effect of moderate hypothermic circulatory arrest on neurological outcomes in elderly patients undergoing replacement of the thoracic aorta
Authors: Salem Mohamed , Friedrich Christine , Thiem Alexander , Salem Mostafa Ahmed , Puehler Thomas , Rusch Rene , Berndt Rouven , Cremer Jochen , Haneya Assad
Published: March 2020

Background

Various studies evaluated the relationship between hypothermic circulatory arrest and neurological outcome in patients undergoing replacement of ascending aorta. The current analysis focuses on the effect of moderate hypothermic circulatory arrest (MHCA) on elderly patients. The aim of our study was to evaluate the impact of MHCA on neurological outcomes in elderly patients undergoing replacement of the ascending aorta.

Results

We retrospectively analyzed 905 consecutive patients, who underwent elective replacement of ascending aorta in MHCA (24 ± 2 °C, nasopharyngeal) between 2001 and 2015. Patients with acute aortic dissection were excluded from this study. Patients were divided into two groups: those aged 75 years and older (elderly group 22.4%,n = 203) and those younger than 75 years (younger group 77.6%,n = 702).

The average age was 63.2 ± 10.2 in the young group vs. 78.7 ± 3.0 years in elderly group (p < 0.001). The elderly group had a significantly higher EuroSCORE II [26.7% (18.1, 36.3) vs. 11.6% (7.4, 19.9);p < 0.001)]. The incidence of coronary heart disease (49.8% vs. 35.6%,p < 0.001) and chronic renal failure (17.2% vs. 9.1%,p = 0.001) was significantly higher in the elderly group. Intraoperatively, the time of MHCA [14 min (12, 17) vs. 15 min (12, 18);p = 0.42], cardiopulmonary bypass [139 min (110, 183) vs. 144 min (113, 189);p = 0.225], and cross-clamping [91 min (63, 116) vs. 92 min (65, 127);p = 0.348] was similar in both groups. Postoperatively, a higher incidence of delirium was significantly reported in the elderly group (24.1% vs. 9.0%,p < 0.001). However, there was no significant difference regarding neurological complications between both groups. A 30-day mortality was acceptable for the elderly group, but significantly higher compared with the younger group (7.1% vs. 3.5%,p = 0.031).

Conclusions

Our study suggests that surgical replacement of the ascending aorta in MHCA can also be applied safely in elderly patients without increasing the risk of severe neurological complications.
Toll-like receptor 9 negatively related to clinical outcome of AML patients
Authors: Al-Kahiry Waiel M. A. , Dammag Enas A. M. , Abdelsalam Hadeel S. T. , Fadlallah Hayat K. , Owais Mona S.
Published: March 2020

Background

Acute myeloid leukemia (AML) can modulate toll-like receptor-9 (TLR9) expression and activation. This study was conducted to elucidate the expression of TLR9 in AML patients and its relation to the prognosis of the disease.

Results

The study included 40 newly diagnosed AML patients managed in the hospital in addition to 20 sex and age matched normal volunteers as control. TLR9 expression assay was conducted on peripheral blood samples of AML cases before the start of treatment as well as the controls by immunophenotyping. TLR9 expression was ranging from 0.10 to 2.40% in AML patients with higher expression among the control, ranging from 0.94 to 8.25%. The median TLR9 expression in AML patients was significantly lower with advanced cytogenetic risk score. It is not significantly differing in relation to patients’ sex, age group, and FAB type of AML. However, significant lower median expression was found in relation to clinical outcome. TLR9 expression ≤ 1% showed lower median overall survival time when compared to those with > 1% expression.

Conclusion

This study concluded that AML patients express TLR9 in leukemic cells with very low percentage. This expression was negatively related to the clinical outcome.
Sustainability of the streamlined ART (START-ART) implementation intervention strategy among ART-eligible adult patients in HIV clinics in public health centers in Uganda: a mixed methods study
Authors: Katuramu Richard , Kamya Moses R. , Sanyu Naome , Armstrong-Hough Mari , Semitala Fred C.
Published: March 2020

Background

Despite increasing access to antiretroviral therapy (ART), the proportion of eligible patients initiated on treatment remains suboptimal. Only 64.6% of the people living with HIV (PLHIV) globally were initiated on ART by June 2019. The streamlined ART (START-ART) implementation study was based on the PRECEDE model, which suggests that “predisposing, enabling, and reinforcing” factors are needed to create behavior change. START-ART increased ART initiation within 2 weeks of eligibility by 42%. However, the gains from some implementation interventions erode over time. We evaluated facilitators and barriers to sustainability of this streamlined ART initiation in the year following the implementation period.

Methods

We designed a mixed-methods explanatory sequential study to examine the sustainability of START-ART implementation. Quantitative component consisted of cross-sectional patient chart reviews of routinely collected data; qualitative component consisted of key informant interviews of health workers in START-ART facilities 2 years after conclusion of the implementation period. We analyzed data from 15 public health centers of Mbarara district, where the START-ART implementation was carried out. We included PLHIV aged > 18 years who initiated ART from June 2013 to July 2016. The START-ART implementation took place from June 2013 to June 2015 while the sustainability period was from August 2015 to July 2016.

Results

A total of 863 ART-eligible patients were sampled. The median CD4 count was 348 cells/ml (IQR 215–450). During the intervention, 338 (77.4%) eligible patients initiated on ART within 2 weeks compared with 375 (88.2%) during the sustainability period (risk difference 10.8%; 95% CI 5.9–15.8%). In 14 of the 15 health centers, the intervention was sustained. During key informant interviews, rapid ART initiation sustainability was attributed to counseling skills that were obtained during intervention and availability of point-of-care (POC) CD4 PIMA machine. Failure to sustain the intervention was attributed to three specific barriers: lack of training after the intervention, transfer of trained staff to other health facilities, and shortage of supplies like cartridges for POC CD4 PIMA machine.

Conclusion

Rapid ART initiation was sustained in most health centers. Skills acquired during the intervention and functional POC CD4 machine facilitated while staff transfers and irregular laboratory supplies were barriers to sustainability of rapid ART initiation.
Resource-Stratified Guideline-Based Cancer Care Should Be a Priority: Historical Context and Examples of Success
Authors: Hunter Natasha , Dempsey Naomi , Tbaishat Fayez , Jahanzeb Mohammad , Al-Sukhun Sana , Gralow Julie R.
Published: March 2020

Low- and middle-income countries (LMICs) are shouldering most of the burden of the rapidly increasing cancer incidence and mortality worldwide, and this situation is projected to worsen in coming decades. Studies estimate that more than one million deaths could be prevented annually if all patients received high-quality care, but most LMICs lack the resources and infrastructure to adopt U.S. or European clinical oncology practice guidelines. Several organizations have developed resource-stratified guidelines (RSGs) to provide graduated and/or region-specific strategies for cancer diagnosis and treatment. The birth of these efforts traces to 2002, when the World Health Organization (WHO) called for tailoring cancer treatments to the level of available resources by country; the Breast Health Global Initiative (BHGI) formalized the first stratified guidelines for breast cancer shortly thereafter. Since then, multiple organizations including ASCO and the National Comprehensive Cancer Network (NCCN) have created guidelines customized for various cancer subtypes and regions. These RSGs offer roadmaps for policy makers, clinicians, and health care administrators in LMICs to design projects in implementation science that can gradually and strategically raise the quality of cancer care in their nation or region. Although the same resource limitations that complicate cancer care in these areas also pose barriers to data gathering and research, some countries have met the challenge and are improving cancer care using RSGs as a metric for success.
The Evolution of Clinical Trials in Metastatic Breast Cancer: Design Features and Endpoints That Matter
Authors: Seidman Andrew D. , Maues Julia , Tomlin Tiah , Bhatnagar Vishal , Beaver Julia A.
Published: March 2020

The evolution of thought in assessing benefit in clinical trials of systemic therapy for metastatic breast cancer (MBC) is well documented, with most agents garnering regulatory approval based either on an advantage in overall survival (OS), time to progression (TTP), or progression-free survival (PFS) over an existing standard of care or objective response rate (ORR). Previous guidance for industry on clinical trial endpoints for the approval of cancer drugs and biologics was provided by the U.S. Food and Drug Administration (FDA) in 2007 and recently updated in 2018. The more recent FDA guidance recognizes that advances in science are facilitating the development of oncology products, which “may also result in the identification of additional endpoints that may be used to support approval of oncology products.” This article critically addressed the evolution of thought on the advancement of clinical trials in MBC, from various stakeholder perspectives. Despite the term “stakeholder,” the objective of all co-authors and parties concerned is to promote and inform the optimal design, conduct, and reporting of clinical trials for women with advanced breast cancer toward improving and extending lives. This article provides an overview of the evolving perspectives on this issue from the physician, regulatory agency, and patient and/or advocate points of view.
Recovery After Stroke
Authors: Cramer Steven C.
Published: March 2020

PURPOSE OF REVIEW

This article describes restorative therapies to improve patient outcomes after stroke. These therapies contrast with acute stroke treatments such as recombinant tissue plasminogen activator (rtPA) and thrombectomy that target clots, aim to salvage threatened brain tissue to limit injury, and have a time window measured in hours. Restorative therapies target the brain, aim to promote plasticity within surviving brain tissue, and have a time window measured in days to weeks or longer.

RECENT FINDINGS

A number of drugs are under study. Preclinical studies are providing attractive therapeutic candidates for translation, such as the C-C chemokine receptor 5 inhibitor maraviroc. Some drug studies have used a pragmatic approach, which is premature for the nascent field of neural repair. Substantial data support the utility of activity-dependent therapies, including constraint-induced movement therapy, with recent studies supporting the need for very high doses to generate the best functional gains. While stem cell therapies are at an early stage, mounting preclinical evidence supports the efficacy of mesenchymal stem cells; some initial human studies are supportive. Several types of brain stimulation have been examined, and in some cases initial studies are promising.

Improved insights into stroke recovery and its treatment have the potential to reduce disability in a large segment of stroke survivors.
Neuroimaging in Acute Stroke
Authors: Menon Bijoy K.
Published: March 2020

PURPOSE OF REVIEW

This article describes how imaging can be used by physicians in diagnosing, determining prognosis, and making appropriate treatment decisions in a timely manner in patients with acute stroke.

RECENT FINDINGS

Advances in acute stroke treatment, including the use of endovascular thrombectomy in patients with large vessel occlusion and, more recently, of IV thrombolysis in an extended time window, have resulted in a paradigm shift in how imaging is used in patients with acute stroke. This paradigm shift, combined with the understanding that “time is brain,” means that imaging must be fast, reliable, and available around the clock for physicians to make appropriate clinical decisions. CT has therefore become the primary imaging modality of choice. Recognition of a large vessel occlusion using CT angiography has become essential in identifying patients for endovascular thrombectomy, and techniques such as imaging collaterals on CT angiography or measuring blood flow to predict tissue fate using CT perfusion have become useful tools in selecting patients for acute stroke therapy. Understanding the use of these imaging modalities and techniques in dealing with an emergency such as acute stroke has therefore become more important than ever for physicians treating patients with acute stroke.

Imaging the brain and the blood vessels supplying it using modern tools and techniques is a key step in understanding the pathophysiology of acute stroke and making appropriate and timely clinical decisions.
Cerebral Small Vessel Disease
Authors: Rost Natalia S. , Etherton Mark
Published: March 2020

PURPOSE OF REVIEW

This article reviews the clinical significance and neuroimaging characteristics of cerebral small vessel disease and the impact on neurologic disease and current and potential therapeutic approaches.

RECENT FINDINGS

Cerebral small vessel disease is increasingly prevalent and highly heterogeneous in neuroimaging and clinical presentation. Small subcortical infarcts, lacunes, cerebral microbleeds, cortical microinfarcts, and white matter hyperintensity of presumed vascular origin represent the major neuroimaging markers of small vessel disease. Increasing small vessel disease burden is associated with risk of incident stroke and dementia, as well as other neuropsychiatric symptoms. Current research strategies are targeting elucidation of the mechanisms of small vessel disease pathogenesis and pursuing clinical trials of therapeutic agents to reduce the clinical manifestations of cerebral small vessel disease.

Cerebral small vessel disease is common in aging adults and represents a major risk factor for multiple acute and chronic neurologic diseases. Increased awareness of cerebral small vessel disease as a modifiable risk factor holds potential for reducing neurologic disease morbidity and mortality across diverse populations in the United States and worldwide.
Auxin fluxes through plasmodesmata modify root-tip auxin distribution
Authors: Mellor Nathan L. , Voß Ute , Janes George , Bennett Malcolm J. , Wells Darren M. , Band Leah R.
Published: March 2020

Auxin is a key signal regulating plant growth and development. It is well established that auxin dynamics depend on the spatial distribution of efflux and influx carriers on the cell membranes. In this study, we employ a systems approach to characterise an alternative symplastic pathway for auxin mobilisation via plasmodesmata, which function as intercellular pores linking the cytoplasm of adjacent cells. To investigate the role of plasmodesmata in auxin patterning, we developed a multicellular model of the Arabidopsis root tip. We tested the model predictions using the DII-VENUS auxin response reporter, comparing the predicted and observed DII-VENUS distributions using genetic and chemical perturbations designed to affect both carrier-mediated and plasmodesmatal auxin fluxes. The model revealed that carrier-mediated transport alone cannot explain the experimentally determined auxin distribution in the root tip. In contrast, a composite model that incorporates both carrier-mediated and plasmodesmatal auxin fluxes re-capitulates the root-tip auxin distribution. We found that auxin fluxes through plasmodesmata enable auxin reflux and increase total root-tip auxin. We conclude that auxin fluxes through plasmodesmata modify the auxin distribution created by efflux and influx carriers.
The Marginal Propensity to Consume over the Business Cycle
Authors: Gross Tal , Notowidigdo Matthew J. , Wang Jialan
Published: March 2020

We estimate how the marginal propensity to consume (MPC) out of liquidity varies over the business cycle. Ten years after a Chapter 7 bankruptcy, the bankruptcy flag is removed from the filer’s credit report, generating an increase in credit score. In the year following flag removal, credit card limits increase by $778 and credit card balances increase by $290, implying an MPC of 0.37. Using cohorts of flag removals, we find that the MPC was 20 to 30 percent higher during the Great Recession, increased during the 2001 recession, and is positively correlated with the local unemployment rate. (JEL E21, E24, E32, G51)
Consumer Imperfect Information and Endogenous Price Rigidity
Authors: L’Huillier Jean-Paul
Published: March 2020

This paper studies the propagation of monetary shocks in an economy featuring a strategic microfoundation for price rigidities. Following an aggregate shock to money, most consumers are initially uninformed. The market for goods is decentralized. Firms are better off delaying the adjustment of prices until enough consumers learn. At the same time, consumers learn from firms that have adjusted prices. The implied endogenous information diffusion follows a Bernoulli differential equation, implying a nonlinear path of learning. Nonlinear learning implies hump-shaped dynamics of output and inflation. A quantitative exercise suggests that these dynamics can be sizable and persistent. (JEL D11, D21, D40, D82, E23, E31)
Implications of Labor Market Frictions for Risk Aversion and Risk Premia
Authors: Swanson Eric T.
Published: March 2020

A flexible labor margin allows households to absorb asset value shocks with changes in hours worked, altering the households’ attitudes toward risk (Swanson 2012). This paper analyzes how frictional labor markets affect that analysis. Risk aversion is higher (i) in countries with more frictional labor markets, (ii ) in recessions, and (iii ) for households that have more difficulty finding a job. Labor market frictions in Europe are large enough to raise risk aversion in those countries. Nevertheless, risk aversion in the United States and Europe is much closer to the frictionless benchmark in Swanson (2012) than to traditional, fixed-labor measures. (JEL D11, D81, E24, E32, J22)
The effectiveness of digital multimedia presentation of trial information on recruitment and retention of patients: Protocol for a study within a trial (SWAT).
Authors: Duane Sinead , Vellinga Akke , Smith Valerie , Tierney Marie , Beecher Claire , Burke Megan , Murphy Andrew W. , Devane Declan
Published: March 2020

Background: Studies within trials (SWATs) present an opportunity to examine design factors that may impact on the successful delivery of trials. One area in need of research is trial recruitment. Recruiting patients to trials is a major challenge facing trialists. Failure to meet recruitment targets can result in delays and underpowered studies. This SWAT evaluates the effectiveness of hand-held digital multimedia presentation of trial information and standard written patient information to potential participants on recruitment and retention to a host trial.

Methods: This is the protocol for SWAT 15, a two-group, embedded parallel randomised controlled trial (RCT) (ISRCTN12838042) designed within a host trial - the SATIN trial (ISRCTN88111427), a RCT designed for implementation in the Irish primary care setting. The SWAT eligibility criteria was determined by the host trial. General practices who agree to participate in the host trial will provide women (participants) who are willing to consider participating in the host trial with either a multimedia digital information resource facilitated through a handheld tablet device, plus a written participant information leaflet (Intervention) or a written participant information leaflet (comparator). Outcomes are recruitment and retention to the host SATIN trial and participant’s quality of decision-making.

Discussion: Although designed to be implemented in a host trial, the host trial, was suspended and therefore this SWAT was not implemented. The protocol and the lessons learnt whilst developing it offer guidance to researchers who wish to answer similar research questions in the future in a similar context or setting.  

Trial registration: ISRCTN Registry ISRCTN12838042 (11/10/2017)
Study protocol: The INTERMAP China Prospective (ICP) study
Authors: Yan Li , Carter Ellison , Fu Yu , Guo Dongshuang , Huang Pinchun , Xie Gaoqiang , Xie Wuxiang , Zhu Yidan , Kelly Frank , Elliott Paul , Zhao Liancheng , Yang Xudong , Ezzati Majid , Wu Yangfeng , Baumgartner Jill , Chan Queenie
Published: March 2020

Background: Unfavourable blood pressure (BP) level is an established risk factor for cardiovascular diseases (CVD), while the exact underlying reasons for unfavourable BP are poorly understood.  The INTERMAP China Prospective (ICP) Study is a prospective cohort to investigate the relationship of environmental and nutritional risk factors with key indicators of vascular function (BP, arterial stiffness, carotid-intima media thickness) among middle-aged/older men and women.

Methods: A total of 839 Chinese participants aged 40-59 years from three diverse regions of China were enrolled in INTERMAP in 1997/98; data collection included repeated BP measurements, 24-hour urine specimens, and 24-hour dietary recalls.  In 2015/16, 574 of these 839 persons were re-enrolled along with 208 new participants aged 40-59 years that were randomly selected from the same study villages.  Participant’s environmental and dietary exposures and health outcomes were assessed in this open cohort study, including BP, 24-hour dietary recalls, personal exposures to air pollution, grip strength, arterial stiffness, carotid-media thickness and plaques, cognitive function, and sleep patterns.  Serum and plasma specimens were collected with 24-hour urine specimens. A follow-up visit has been scheduled for 2020-2021.

Discussion:  Winter and summer assessments of a comprehensive set of vascular indicators and their environmental and nutritional risk factors were conducted with high precision.  We will leverage advances in exposome research to identify biomarkers of exposure to environmental and nutritional risk factors and improve our understanding of the mechanisms and pathways of their hazardous cardiovascular effects.  The ICP Study is observational by design, thus subject to several biases including selection bias (e.g., loss to follow-up), information bias (e.g., measurement error), and confounding that we sought to mitigate through our study design and measurements.  However, extensive efforts will apply to minimize those limitations (continuous observer training, repeated measurements of BP, standardized methods in data collection and measurements, and on-going quality control).
Hamiltonian Monte Carlo sampling to estimate past population dynamics using the skygrid coalescent model in a Bayesian phylogenetics framework
Authors: Baele Guy , Gill Mandev S. , Lemey Philippe , Suchard Marc A.
Published: March 2020

Nonparametric coalescent-based models are often employed to infer past population dynamics over time. Several of these models, such as the skyride and skygrid models, are equipped with a block-updating Markov chain Monte Carlo sampling scheme to efficiently estimate model parameters. The advent of powerful computational hardware along with the use of high-performance libraries for statistical phylogenetics has, however, made the development of alternative estimation methods feasible. We here present the implementation and performance assessment of a Hamiltonian Monte Carlo gradient-based sampler to infer the parameters of the skygrid model. The skygrid is a popular and flexible coalescent-based model for estimating population dynamics over time and is available in BEAST 1.10.5, a widely-used software package for Bayesian pylogenetic and phylodynamic analysis. Taking into account the increased computational cost of gradient evaluation, we report substantial increases in effective sample size per time unit compared to the established block-updating sampler. We expect gradient-based samplers to assume an increasingly important role for different classes of parameters typically estimated in Bayesian phylogenetic and phylodynamic analyses.
Public activities preceding the onset of acute respiratory infection syndromes in adults in England - implications for the use of social distancing to control pandemic respiratory infections.
Authors: Hayward Andrew C. , Beale Sarah , Johnson Anne M. , Fragaszy Ellen B. ,   Flu Watch Group
Published: March 2020

Background: Social distancing measures may reduce the spread of emerging respiratory infections however, there is little empirical data on how exposure to crowded places affects risk of acute respiratory infection.

Methods: We used a case-crossover design nested in a community cohort to compare self-reported measures of activities during the week before infection onset and baseline periods. The design eliminates the effect of non-time-varying confounders. Time-varying confounders were addressed by exclusion of illnesses around the Christmas period and seasonal adjustment. 

Results: 626 participants had paired data from the week before 1005 illnesses and the week before baseline. Each additional day of undertaking the following activities in the prior week was associated with illness onset: Spending more than five minutes in a room with someone (other than a household member) who has a cold (Seasonally adjusted OR 1·15, p=0·003); use of underground trains (1·31, p=0·036); use of supermarkets (1·32, p<0·001); attending a theatre, cinema or concert (1·26, p=0·032); eating out at a café, restaurant or canteen (1·25, p=0·003); and attending parties (1·47, p<0·001). Undertaking the following activities at least once in the previous week was associated with illness onset: using a bus, (aOR 1.48, p=0.049), shopping at small shops (1.9, p<0.002) attending a place of worship (1.81, p=0.005).   

Conclusions: Exposure to potentially crowded places, public transport and to individuals with a cold increases risk of acquiring circulating acute respiratory infections. This suggests social distancing measures can have an important impact on slowing transmission of emerging respiratory infections.
Seasonality and immunity to laboratory-confirmed seasonal coronaviruses (HCoV-NL63, HCoV-OC43, and HCoV-229E): results from the Flu Watch cohort study
Authors: Aldridge Robert W. , Lewer Dan , Beale Sarah , Johnson Anne M. , Zambon Maria , Hayward Andrew C. , Fragaszy Ellen B. ,   Flu Watch Group
Published: March 2020

Background: There is currently a pandemic caused by the novel coronavirus SARS-CoV-2. The intensity and duration of this first wave in the UK may be dependent on whether SARS-CoV-2 transmits more effectively in the winter than the summer and the UK Government response is partially built upon the assumption that those infected will develop immunity to reinfection in the short term. In this paper we examine evidence for seasonality and immunity to laboratory-confirmed seasonal coronavirus (HCoV) from a prospective cohort study in England.

Methods: In this analysis of the Flu Watch cohort, we examine seasonal trends for PCR-confirmed coronavirus infections (HCoV-NL63, HCoV-OC43, and HCoV-229E) in all participants during winter seasons (2006-2007, 2007-2008, 2008-2009) and during the first wave of the 2009 H1N1 influenza pandemic (May-Sep 2009). We also included data from the pandemic and ‘post-pandemic’ winter seasons (2009-2010 and 2010-2011) to identify individuals with two confirmed HCoV infections and examine evidence for immunity against homologous reinfection.

Results: We tested 1,104 swabs taken during respiratory illness and detected HCoV in 199 during the first four seasons. The rate of confirmed HCoV infection across all seasons was 390 (95% CI 338-448) per 100,000 person-weeks; highest in the Nov-Mar 2008/9 season at 674 (95%CI 537-835). The highest rate was in February at 759 (95% CI 580-975). Data collected during May-Sep 2009 showed there was small amounts of ongoing transmission, with four cases detected during this period. Eight participants had two confirmed infections, of which none had the same strain twice.

Conclusion: Our results provide evidence that HCoV infection in England is most intense in winter, but that there is a small amount of ongoing transmission during summer periods. We found some evidence of immunity against homologous reinfection.
Investigating the influence of geometry of the heliospheric neutral current sheet and solar activity on modulation of galactic cosmic rays with a method of main components
Authors: Gololobov Peter , Krivoshapkin Prokopy , Krymsky Germogen , Gerasimova Sardaana
Published: March 2020

The work studies the cumulative modulating effect of the geometry of the interplanetary magnetic field's neutral current sheet and solar activity on propagation of galactic cosmic rays in the heliosphere. The role of each factor on the modulation of cosmic rays is estimated using a method of main components. The application of the method to experimental data on solar activity, to the tilt angle of the neutral sheet, and cosmic ray intensity for a long period from 1980 to 2018 allows us to reveal the temporal dynamics of roles of these factors in the modulation. The modulation character is shown to strongly depend on the polarity of the Sun’s general magnetic field. Results of the study confirm the existing theoretical concepts of the heliospheric modulation of cosmic rays and reflect its peculiarities for almost four full cycles of solar activity.
Galactic cosmic ray anisotropy modelling
Authors: Peregoudov Dmitriy , Soloviev Anatoly , Yashin Igor , Shutenko Victor
Published: March 2020

We calculate the angular distribution of cosmic rays at a given point of the heliosphere under the assumption that the incoming flux from outer space is isotropic. The static magnetic field is shown to cause no anisotropy provided that the observation point is situated out of the trapped particle area. We consider a coronal ejection model in the form of a static cylinder with an axial homogeneous magnetic field inside. We calculate angular distribution samples in the trapped particle area (inside the cylinder) and show that there is a certain cone of directions with a reduced flux. For the same model with the moving cylinder, the angular distribution samples are calculated for different positions of the observation point outside the cylinder. Anisotropy of order of the ejection to light velocity ratio is shown to arise. The calculated samples are in qualitative agreement with URAGAN muon hodoscope data.
Ionospheric disturbances over Eastern Siberia during April 12–15, 2016 geomagnetic storms
Authors: Rubtsov Aleksandr , Maletckii Boris , Danilchuk Ekaterina , Smotrova Ekaterina , Shelkov Aleksei , Yasyukevich Anna
Published: March 2020

We present the results of the complex study of ionospheric parameter variations during two geomagnetic storms, which occurred on April 12–15, 2016. The study is based on data from a set of radiophysical and optical instruments. Both the storms with no sudden commencement were generated by high-speed streams from a coronal hole. Despite the minor intensity of the storms (Dst ≥ –55 and –59 nT), we have revealed a distinct ionospheric response to these disturbances. A negative response of electron density and F2-layer critical frequency was observed during the main phase of both the storms. The amplitude of the negative response was higher for the second storm. The period of negative electron density deviations was accompanied by an increase in the peak height, as well as by the downward plasma drift in the evening and night hours, which is not typical of quiet conditions. We have also recorded sharp peaks in the AATR (Along Arc TEC Rate) index and in total electron content noise spikes on average 2–2.5 times. This indicates an intensification of small-scale ionospheric disturbances caused by disturbed geomagnetic conditions and high substorm activity.
Analyzing existing applied models of the ionosphere for calculating radio wave propagation and possibility of their use for radar systems. I. Classification of applied models and the main requirements imposed on them for radar aids
Authors: Aksenov Oleg , Kozlov Stanislav , Lyakhov Andrey , Trekin Vyacheslav , Perunov Yuriy , Yakubovsky Sergey
Published: March 2020

We review modern HF–X band radars covering over-the-horizon problems. The ionosphere significantly affects wave propagation in all the bands. We describe available correction techniques, which use additional evidence on the ionosphere, as well as models of different degrees of complexity. The fact that the field of view cannot be covered by ground-based instruments as well as the growing requirements to the precision and stability of the radars result in the impossibility of ionospheric correction with up-to-date models, hence the latter require further elaboration. We give a virtually full classification of the models. The article summarizes the requirements to the models for the radars depending on their function.
Features of Ne recovery at the Irkutsk Incoherent Scatter Radar
Authors: Alsatkin Sergei , Medvedev Andrey , Ratovsky Konstantin
Published: March 2020

The article presents a new method of restoring the electron density profile (Ne) according to data from the Irkutsk Incoherent Scatter Radar (IISR). This method has been developed taking into account the Faraday rotation of the polarization plane, which leads to fading at the output of the IISR linearly polarized antenna. The concept of the method consists in fitting a height variation of electron density by a parametric model. As the model, a combination of two Chapman layers was used. This approach made it possible to implement a fully automatic data processing mode and increase the stability of the recovery of the Ne profile, especially according to data obtained during a period of low solar activity when the signal-to-noise ratio is low. Accuracy was increased by eliminating a number of operations leading to instability of data recovery in the presence of noise. The new method enabled fully automatic processing of long data series in the period 2007–2015.
Effect of the Sun on Earth’s seismicity
Authors: Guglielmi Anatol , Klain Boris
Published: March 2020

For the experimental study of the relationship between earthquakes and solar activity, we introduce the idea of the statistical sum Z of earthquake ensemble. A number of numerical parameters of seismicity (average planetary magnitude, entropy, fluctuations of magnitude) are expressed through Z. A severe restriction is, however, imposed on the magnitude during the formation of the ensemble. We propose an alternative method. It does not have this specific restriction, although it allows us to calculate only one numerical parameter, namely the global daily magnitude Mg. Over the 20-year period from 1980 to 1999, 7300 values of Mg have been calculated. The comparison between the Mg values and Wolf numbers W made it possible to determine the effect of the Sun on earthquakes at a statistically significant level.
Astroclimatic statistics at the Sayan Solar Observatory
Authors: Shikhovtsev Artem , Kovadlo Pavel , Kiselev Aleksandr
Published: March 2020

The paper analyzes meteorological and optical characteristics of the atmosphere at the Sayan Solar Observatory (SSO) and the future 3 m Large Solar Telescope (LST-3). We examine spatial features of changes in astroclimatic characteristics for the Sayan Solar Observatory and Baikal Astrophysical Observatory (BAO). We have obtained a vertical profile of the structural characteristic of air refractive index fluctuations for a low intensity optical turbulence along the line of sight. This profile is an important result because it will allow us to adjust the adaptive optics system of LST-3 to the best astroclimatic conditions when the correction efficiency is maximal. In order to analyze vertical profiles of optical turbulence characteristics and to assess the contribution of individual atmospheric layers to the isoplanatic angle for a minimum level of total turbulence, we give recommendations for the design of multi-conjugated adaptive optics in general and for LST-3 in particular.
Psychogenic and behavior risks in postoperative period in maxillofacial surgery
Authors: Semenov D. M.
Published: March 2020

Introduction. Maxillofacial trauma is one of the most relevant surgical issues in modern urban society. The issues of correction of post-traumatic pain and functional disorders, as well as prevention of psychological and behavioral violations in maxillofacial surgery patients creates specific interdisciplinary surgical and psychological problem. Aim – to systematize psychological and behavioral violations caused by stress associated with maxillofacial trauma and to evaluate its impact on postoperative treatment. Materials and methods. The study conducted in a prospective design during 2016-2018. 120 patients with maxillofacial area fractures, undergoing reconstructive surgery at City Clinical Hospital of Emergency and Rapid Care (Zaporizhzhya, Ukraine) were examined. Results. Predictors, psychological manifestations and influence on the in- and outpatient stages of the postoperative period of mixed negative emotional reactions on TAS; psychomotor reactions on TAS; somato-functional reactions on TAS; dental response on TAS; behavioral reactions on TAS; psychosensory reactions on TAS, personality reactions to changes in self-perception; personality reactions to social dysfunction; personality reactions to the violation of family status; personality reactions to communicative disorders;  personality reactions to quality of life decrease; personality reactions to general condition violations; personality reactions to existential distress are described. Conclusions. Psychological and behavioral violations caused by stress associated with maxillofacial trauma are systematized, 6 main components of trauma-associated psychosocial maladaptation and 7 main components of defect-associated psychosocial maladaptation are described.