bluuebunny/crossref_metadata_2025 huggingface dataset:
Anti-Inflammatory Halogenated Monoterpenes from the Red Alga Portieria hornemannii
Authors: Wu Yuan-Jhong , Huang Tzu-Yin , Huang Chiung-Yao , Lin Chi-Chen , Wang Wei-Lung , Huang Hui-Chi , Liu Shang-Yin Vanson , Chao Chih-Hua , Sheu Jyh-Horng
Published: September 2023

The chemical investigation of a red alga Portieria hornemannii enabled the identification of three new halogenated monoterpenes (1–3) along with two previously identified metabolites (4 and 5). Their structures were determined by spectroscopic analysis and also by utilizing single-crystal diffraction analysis and quantum chemical calculation, as well as by comparison with literature data. Further corrections for dichloro and dibromo carbons using the sorted training set (STS) method were established in this study to significantly improve the accuracy in GIAO 13C NMR calculation of compounds 1–3. To discover the potential bioactive metabolites from P. hornemannii, the anti-inflammatory activities of all compounds were examined. Compounds 1 and 3–5 showed significant anti-inflammatory activity to inhibit the production of pro-inflammatory cytokines in the LPS-stimulated mature dendritic cells.
Rifamycin-Related Polyketides from a Marine-Derived Bacterium Salinispora arenicola and Their Cytotoxic Activity
Authors: Van Anh Cao , Kang Jong Soon , Yang Jeong-Wook , Kwon Joo-Hee , Heo Chang-Su , Lee Hwa-Sun , Shin Hee Jae
Published: September 2023

Eight rifamycin-related polyketides were isolated from the culture broth of a marine-derived bacterium Salinispora arenicola, including five known (2–5 and 8) and three new derivatives (1, 6, and 7). The structures of the new compounds were determined by means of spectroscopic methods (HRESIMS and 1D, 2D NMR) and a comparison of their experimental data with those previously reported in the literature. The isolated compounds were evaluated for their cytotoxicity against one normal, six solid, and seven blood cancer cell lines and 1 showed moderate activity against all the tested cell lines with GI50 values ranging from 2.36 to 9.96 µM.
A Study on the Formation of Fiber Texture in the Subsurface Layer of Hot-Rolled Plate of 3.2%Si Grain-Oriented Steel
Authors: Zou Xiang , Liu Qingyou , Qiu Shengtao
Published: September 2023

During the hot rolling process, inhomogeneity microstructure and variations of texture along the thickness direction of hot-rolled plate were formed through shear deformation and recrystallization. The purpose of this study is to investigate the formation and evolution of fiber texture in hot-rolled plate of grain-oriented silicon steel. The results indicate that, in the surface layer s = 1 of hot-rolled plate, a large number of equiaxed grains are formed under high shear force. Under the surface layer, as shear force gradually decreases along the thickness direction, the grain aspect ratio of recrystallized grains gradually increases along the thickness direction of hot-rolled plate, and the shape of the recrystallized grains formed in the subsurface layer and center layer becomes more irregular along the thickness direction. Under the combination of compressive force and decreasing shear force along the thickness direction, fiber texture composed of dozens of specific orientations (such as {112}<111>, {110}<112>, {213}<364>, {441}<104> and Goss orientations) is formed in the subsurface layer s = 0.5~0.9 and gradually evolves along the thickness direction. Therefore, the formation of Goss texture is a small part of fiber texture and is the result of texture evolution in the subsurface layer s = 0.5~0.9.
Clustering Disease of Clostridioides Difficile Infection: Implication for the Management in Internal Medicine
Authors: Crispino Pietro
Published: September 2023

Clostridioides difficile is a bacterium responsible for a healthcare-associated gastrointestinal infection, primarily affecting people who have undergone prolonged antibiotic treatment or who have compromised immune systems. The CD is of particular concern due to its high recurrence rates and the potential for serious outcomes, including life-threatening conditions such as pseudomembranous colitis, septic shock, and all associated conditions. Since this infection is a disease associated with other health conditions, a general vision of the problems is necessary which aims to obtain a general overview of the manifestations that generally correlate with care. Clinical reasoning following the disease-clustering method is able to produce a categorization process by grouping the possible correlations of the various conditions or factors underlying diseases on the basis of certain similarities or common models. The clustering process is performed using data analysis techniques which, by statically correlating each other, give an exact dimension of all the information related to a particular disease. In the case of CD, reasoning based on disease clustering has better clarified the practices, appropriateness in infection control, judicious use of antibiotics, and research into therapeutic and preventive strategies. This review, taking advantage of the clustering strategy, aimed to analyze the contingent conditions of the infection under examination, to reduce the incidence and impact of CD, having as its mission the improvement of the results deriving from the contrast of all those correlated pathological conditions to healthcare for the improvement of public health.
Achieving Discharge Limits in Single-Stage Domestic Wastewater Treatment by Combining Urban Waste Sources and Phototrophic Mixed Cultures
Authors: Chacon-Aparicio Sandra , Villamil John Alexander , Martinez Fernando , Melero Juan Antonio , Molina Raul , Puyol Daniel
Published: September 2023

This work shows the potential of a new way of co-treatment of domestic wastewater (DWW) and a liquid stream coming from the thermal hydrolysis of the organic fraction of municipal solid waste (OFMSW) mediated by a mixed culture of purple phototrophic bacteria (PPB) capable of assimilating carbon and nutrients from the medium. The biological system is an open single-step process operated under microaerophilic conditions at an oxidative reduction potential (ORP) < 0 mV with a photoperiod of 12/24 h and fed during the light stage only so the results can be extrapolated to outdoor open pond operations by monitoring the ORP. The effluent mostly complies with the discharge values of the Spanish legislation in COD and p-values (<125 mg/L; <2 mg/L), respectively, and punctually on values in N (<15 mg/L). Applying an HRT of 3 d and a ratio of 100:7 (COD:N), the presence of PPB in the mixed culture surpassed 50% of 16S rRNA gene copies, removing 78% of COD, 53% of N, and 66% of P. Furthermore, by increasing the HRT to 5 d, removal efficiencies of 83% of COD, 65% of N, and 91% of P were achieved. In addition, the reactors were further operated in a membrane bioreactor, thus separating the HRT from the SRT to increase the specific loading rate. Very satisfactory removal efficiencies were achieved by applying an HRT and SRT of 2.3 and 3 d, respectively: 84% of COD, 49% of N, and 93% of P despite the low presence of PPB due to more oxidative conditions, which step-by-step re-colonized the mixed culture until reaching >20% of 16S rRNA gene copies after 49 d of operation. These results open the door to scaling up the process in open photobioreactors capable of treating urban wastewater and municipal solid waste in a single stage and under microaerophilic conditions by controlling the ORP of the system.
Assessment of Charge Initiation Techniques Effect on Blast Fragmentation and Environmental Safety: An Application of WipFrag Software
Authors: Taiwo Blessing Olamide , Fissha Yewuhalashet , Palangio Thomas , Palangio Andrew , Ikeda Hajime , Cheepurupalli Nageswara Rao , Khan Naseer Muhammad , Akinlabi Adams Abiodun , Famobuwa Oluwaseun Victor , Faluyi Joshua Oluwaseyi , Kawamura Youhei
Published: September 2023

Blast charge initiation procedures have a significant impact on both mining safety and production rates. In this study, the inventory benefit of an electric initiation system was investigated to assess its influence on both fragmentation and blast-induced damages. The WipFrag software was used to examine the size distribution and productivity of 12 small-scale blasts initiated by both nonelectric and electric detonators. All blast rounds were initiated with plain-type electric and NONEL detonators. The average burden, spacing, stemming length, and charge weight were, respectively, 0.85 m, 1.10 m, 0.66 m, and 1.1 kg. The results showed that the mesh through which 80% of the blast fragments passed for the electric blast was smaller than the mesh through which the material products from the NONEL blast passed. The results also demonstrated that the generated blast-induced ground vibration (PPV) from all blast rounds for electric blast varied from 0.4–1.2 mm/s and 80–105 dB, while that for nonelectric blast ranged from 0.05–0.2 mm/s and 72–95 dB. As a result, the electric blast initiation technique was found to produce good fragmentation, with a higher percentage of optimum fragment sizes on spec than nonelectrically initiated blasts.
Recent Advances in the Use of Diorganyl Diselenides as Versatile Catalysts
Authors: da Costa Gabriel Pereira , Blödorn Gustavo Bierhals , Barcellos Angelita Manke , Alves Diego
Published: September 2023

The importance of organoselenium compounds has been increasing in synthetic chemistry. These reagents are well-known as electrophiles and nucleophiles in many organic transformations, and in recent years, their functionality as catalysts has also been largely explored. The interest in organoselenium-based catalysts is due to their high efficacy, mild reaction conditions, strong functional compatibility, and great selectivity. Allied to organoselenium catalysts, the use of inorganic and organic oxidants that act by regenerating the catalytic species for the reaction pathway is common. Here, we provide a comprehensive review of the last five years of organic transformations promoted by diorganyl diselenide as a selenium-based catalyst. This report is divided into four sections: (1) cyclisation reactions, (2) addition reactions and oxidative functionalisation, (3) oxidation and reduction reactions, and (4) reactions involving phosphorus-containing starting materials.
Ochratoxin A—The Current Knowledge Concerning Hepatotoxicity, Mode of Action and Possible Prevention
Authors: Więckowska Magdalena , Szelenberger Rafał , Niemcewicz Marcin , Harmata Piotr , Poplawski Tomasz , Bijak Michał
Published: September 2023

Ochratoxin A (OTA) is considered as the most toxic of the other ochratoxins synthesized by various fungal species belonging to the Aspergillus and Penicillium families. OTA commonly contaminates food and beverages, resulting in animal and human health issues. The toxicity of OTA is known to cause liver damage and is still being researched. However, current findings do not provide clear insights into the toxin mechanism of action. The current studies focusing on the use of potentially protective compounds against the effects of the toxin are insufficient as they are mainly conducted on animals. Further research is required to fill the existing gaps in both fields (namely the exact OTA molecular mechanism and the prevention of its toxicity in the human liver). This review article is a summary of the so far obtained results of studies focusing on the OTA hepatotoxicity, its mode of action, and the known approaches of liver cells protection, which may be the base for expanding other research in near future.
A Combined Experimental and Computational Study of Novel Benzotriazinone Carboxamides as Alpha-Glucosidase Inhibitors
Authors: Khalid Zunera , Shafqat Syed Salman , Ahmad Hafiz Adnan , Munawar Munawar Ali , Mutahir Sadaf , Elkholi Safaa M. , Shafqat Syed Rizwan , Huma Rahila , Asiri Abdullah Mohammed
Published: September 2023

Diabetes is a chronic metabolic disorder of the endocrine system characterized by persistent hyperglycemia appears due to the deficiency or ineffective use of insulin. The glucose level of diabetic patients increases after every meal and medically recommended drugs are used to control hyperglycemia. Alpha-glucosidase inhibitors are used as antidiabetic medicine to delay the hydrolysis of complex carbohydrates. Acarbose, miglitol, and voglibose are commercial drugs but patients suffer side effects of flatulence, bloating, diarrhea, and loss of hunger. To explore a new antidiabetic drug, a series of benzotriazinone carboxamides was synthesized and their alpha-glucosidase inhibition potentials were measured using in vitro experiments. The compounds 14k and 14l were found to be strong inhibitors compared to the standard drug acarbose with IC50 values of 27.13 ± 0.12 and 32.14 ± 0.11 μM, respectively. In silico study of 14k and 14l was carried out using molecular docking to identify the type of interactions developed between these compounds and enzyme sites. Both potent compounds 14k and 14l exhibited effective docking scores by making their interactions with selected amino acid residues. Chemical hardness and orbital energy gap values were investigated using DFT studies and results depicted affinity of 14k and 14l towards biological molecules. All computational findings were found to be in good agreement with in vitro results.
Meldrum’s Acid Furfural Conjugate MAFC: A New Entry as Chromogenic Sensor for Specific Amine Identification
Authors: Zeußel Lisa , Singh Sukhdeep
Published: September 2023

Bioactive amines are highly relevant for clinical and industrial application to ensure the metabolic status of a biological process. Apart from this, generally, amine identification is a key step in various bioorganic processes ranging from protein chemistry to biomaterial fabrication. However, many amines have a negative impact on the environment and the excess intake of amines can have tremendous adverse health effects. Thus, easy, fast, sensitive, and reliable sensing methods for amine identification are strongly searched for. In the past few years, Meldrum’s acid furfural conjugate (MAFC) has been extensively explored as a starting material for the synthesis of photoswitchable donor–acceptor Stenhouse adducts (DASA). DASA formation hereby results from the rapid reaction of MAFC with primary and secondary amines, which has so far been demonstrated through numerous publications for different applications. The linear form of the MAFC-based DASA exhibits intense pink coloration due to its linear conjugated triene-2-ol conformation, which has inspired researchers to use this easy synthesizable molecule as an optical sensor for primary, secondary, and biogenic amines. Due to its new entry into amine identification, a collection of the literature exclusively on MAFC is demanded. In this mini review, we intend to present the state-of-the-art of MAFC as an optical molecular sensor in hopes to motivate researchers to find even more applications of MAFC-based sensors and methods that pave the way to their usage in medicinal applications.
Multifunctional Textiles with Flame Retardant and Antibacterial Properties: A Review
Authors: Jin Liping , Ji Chenpeng , Chen Shun , Song Zhicong , Zhou Juntong , Qian Kun , Guo Wenwen
Published: September 2023

It is well known that bacterial infections and fire-hazards are potentially injurious in daily life. With the increased security awareness of life and properties as well as the improvement of living standards, there has been an increasing demand for multifunctional textiles with flame retardant and antibacterial properties, especially in the fields of home furnishing and medical protection. So far, various treatment methods, including the spray method, the dip-coating method, and the pad-dry-cure method, have been used to apply functional finishing agents onto fabrics to achieve the functionalization in the past exploration stage. Moreover, in addition to the traditional finishing technology, a number of novel technologies have emerged, such as layer-by-layer (LBL) deposition, the sol-gel process, and chemical grafting modification. In addition, some natural biomasses, including chitin, chitosan (CS), and several synthetic functional compounds that possess both flame-retardant and bacteriostatic properties, have also received extensive attention. Hence, this review focuses on introducing some commonly used finishing technologies and flame retardant/antibacterial agents. At the same time, the advantages and disadvantages of different methods and materials were summarized, which will contribute to future research and promote the development and progress of the industry.
A Combined NMR and UV–Vis Approach to Evaluate Radical Scavenging Activity of Rosmarinic Acid and Other Polyphenols
Authors: Kola Arian , Vigni Ginevra , Baratto Maria Camilla , Valensin Daniela
Published: September 2023

Oxidative stress results from an imbalance between reactive oxygen species (ROS) production and the body’s ability to neutralize them. ROS are reactive molecules generated during cellular metabolism and play a crucial role in normal physiological processes. However, excessive ROS production can lead to oxidative damage, contributing to various diseases and aging. This study is focused on rosmarinic acid (RA), a hydroxycinnamic acid (HCA) derivative well known for its antioxidant activity. In addition, RA has also demonstrated prooxidant behavior under specific conditions involving high concentrations of transition metal ions such as iron and copper, high pH, and the presence of oxygen. In this study, we aim to clarify the underlying mechanisms and factors governing the antioxidant and prooxidant activities of RA, and to compare them with other HCA derivatives. UV–Vis, NMR, and EPR techniques were used to explore copper(II)’s binding ability of RA, caffeic acid, and p-coumaric acid. At the same time, UV–Vis and NMR methods were exploited to evaluate the polyphenols’ free radical scavenging abilities towards ROS generated by the ascorbic acid–copper(II) system. All the data indicate that RA is the most effective polyphenol both in copper binding abilities and ROS protection.
Metal Recovery from Natural Saline Brines with an Electrochemical Ion Pumping Method Using Hexacyanoferrate Materials as Electrodes
Authors: Salazar-Avalos Sebastian , Soliz Alvaro , Cáceres Luis , Conejeros Sergio , Brito Iván , Galvez Edelmira , Galleguillos Madrid Felipe M.
Published: September 2023

The electrochemical ion pumping device is a promising alternative for the development of the industry of recovering metals from natural sources—such as seawater, geothermal water, well brine, or reverse osmosis brine—using electrochemical systems, which is considered a non-evaporative process. This technology is potentially used for metals like Li, Cu, Ca, Mg, Na, K, Sr, and others that are mostly obtained from natural brine sources through a combination of pumping, solar evaporation, and solvent extraction steps. As the future demand for metals for the electronic industry increases, new forms of marine mining processing alternatives are being implemented. Unfortunately, both land and marine mining, such as off-shore and deep sea types, have great potential for severe environmental disruption. In this context, a green alternative is the mixing entropy battery, which is a promising technique whereby the ions are captured from a saline natural source and released into a recovery solution with low ionic force using intercalation materials such as Prussian Blue Analogue (PBA) to store cations inside its crystal structure. This new technique, called “electrochemical ion pumping”, has been proposed for water desalination, lithium concentration, and blue energy recovery using the difference in salt concentration. The raw material for this technology is a saline solution containing ions of interest, such as seawater, natural brines, or industrial waste. In particular, six main ions of interest—Na+, K+, Mg2+, Ca2+, Cl−, and SO42−—are found in seawater, and they constitute 99.5% of the world’s total dissolved salts. This manuscript provides relevant information about this new non-evaporative process for recovering metals from aqueous salty solutions using hexacianometals such as CuHCF, NiHCF, and CoHCF as electrodes, among others, for selective ion removal.
Exploration of NIR Squaraine Contrast Agents Containing Various Heterocycles: Synthesis, Optical Properties and Applications
Authors: Sarasiya Shahir , Sarasiya Sara , Henary Maged
Published: September 2023

Squaraine dye is a popular class of contrast near-infrared (NIR) dyes. Squaraine dyes have shown the ability to be modified with various heterocycles. The indole moiety is the most notable heterocycle incorporated in squaraine dyes. A tremendous amount of work has gone into developing indole-based squaraine dyes and determining their applications. The optical properties of squaraine dyes containing an indole moiety facilitate high quantum yields and molar absorptivity, but the absorbance maxima is capped near 700 nm. This is the major limitation of indole-based squaraine dyes. In comparison, other heterocycles with larger conjugated systems such as quinoline and perimidine have demonstrated promising optical properties and immense potential for modifications, albeit with limited development. Quinoline- and perimidine-based squaraine dyes have molar extinction coefficients over 100,000 M−1 cm−1 and absorbances over 800 nm. This report will look at indole-, quinoline-, and perimidine-based squaraine dyes. Due to the sheer number of reported dyes, the search for indole-based squaraine dyes has been limited to reports from the past five years (2018–2023). For quinoline- and perimidine-based squaraine dyes, a holistic search was performed to analyze the optical properties and applications, due to the abovementioned limitation. This report will evaluate the three different classes of squaraines: indole-, quinoline-, and perimidine-based, to evaluate their optical properties and applications, with the goal of encouraging the exploration of other heterocycles for use in squaraine dyes.
Fungi Fibrinolytic Compound 1 Plays a Core Role in Modulating Fibrinolysis, Altering Plasma Clot Structure, and Promoting Susceptibility to Lysis
Authors: Gao Chunli , Bao Bin , Bao Chunling , Wu Wenhui
Published: September 2023

Fibrin clot structure and function are major determinants of venous and arterial thromboembolic diseases, as well as the key determinants of the efficiency of clot lysis. Studies have revealed that fungi fibrinolytic compound 1 (FGFC1) is a novel marine pyranisoindolone natural product with fibrinolytic activity. Here, we explore the impacts of FGFC1 on clot structure, lysis, and plasminogen activation in vitro using turbidimetric, enzyme-linked immunosorbent assay, confocal and electron microscopy, urokinase, or plasmin chromogenic substrate. Clots formed in the presence of FGFC1 expressed reduced fibrin polymerization rate and maximum turbidity; however, they did not influence the lag phase of fibrin polymerization. In the absence of scu-PA (single-chain urokinase plasminogen activator), microscopy revealed that FGFC1 increased the number of protofibrils within fibrin fiber and the pore diameter between protofibrils, inducing clots to form a region of thinner and looser networks separated by large pores. The effects of FGFC1 on scu-PA-mediated plasma clot structure were similar to those in the absence of scu-PA. In addition, FGFC1 promoted the lysis of clots and increased the D-dimer concentration in lysate. FGFC1 increased the generation rate of p-nitroaniline in plasma. These results show that FGFC1 has fibrinolytic activity in plasma, leading to interference with the release of fibrinopeptide B to affect lateral aggregation of protofibrils and increase clot susceptibility to fibrinolysis by altering its structure.
Nature, Artifice, and Discovery in Descartes’ Mechanical Philosophy
Authors: Brown Deborah Jean
Published: September 2023

It is often assumed that in the collapse of the Aristotelian distinction between art and nature that results from the rise of mechanical philosophies in the early modern period, the collapse falls on the side of art. That is, all of the diversity among natures that was explained previously as differences among substantial forms came to be seen simply as differences in arrangements of matter according to laws instituted by the “divine artificer”, God. This paper argues that, for René Descartes, the collapse occurs on both sides. Natures are artefacts of God, and human artefacts, under some conditions, can be classified as natures or, at least, continuous within nature. Drawing on developments across both horticulture and engineering in the 16th and 17th centuries, as well as Descartes’ mechanical philosophy, this paper explores challenges to the Aristotelian nature/art distinction. The question then is what, in the advent of this collapse, are human artificers doing when they construct artefacts? Are they replicating God’s powers by creating new natures, or are they doing something else, and if so, what might that be? It is argued that we should view human invention for Descartes not as creating new natures so much as discovering them. These findings have consequences for how we interpret Descartes’ use of the term “nature” in relation to automata and other artefacts produced by human hands.
Sustainable Bioplastics for Food Packaging Produced from Renewable Natural Sources
Authors: Navasingh Rajesh Jesudoss Hynes , Gurunathan Manoj Kumar , Nikolova Maria P. , Królczyk Jolanta B.
Published: September 2023

It is crucial to find an effective, environmentally acceptable solution, such as bioplastics or biodegradable plastics, to the world’s rising plastics demand and the resulting ecological destruction. This study has focused on the environmentally friendly production of bioplastic samples derived from corn starch, rice starch, and tapioca starch, with various calcium carbonate filler concentrations as binders. Two different plasticizers, glycerol and sorbitol, were employed singly and in a rich blend. To test the differences in the physical and chemical properties (water content, absorption of moisture, water solubility, dissolution rate in alcohol, biodegradation in soil, tensile strength, elastic modulus, and FT-IR) of the produced samples, nine samples from each of the three types of bioplastics were produced using various ratios and blends of the fillers and plasticizers. The produced bioplastic samples have a multitude of features that make them appropriate for a variety of applications. The test results show that the starch-based bioplastics that have been suggested would be a better alternative material to be used in the packaging sectors.
Application of Biological Glue–Clay Composite Substrate in Slope Ecological Restoration
Authors: Zhu Xufen , Zheng Jiaqiang , Gao Yuliang , Xue Jian , Hu Guochang , Che Wenyue , Song Zezhuo , Liu Jin , Huang Tingwei , Wu Peng
Published: September 2023

Given the issues of soil cracking, poor water retention during drought, and erosion damage caused by rainfall, we conducted an in-depth study on the water retention properties, cracking resistance, and scouring resistance of biogel-amended clay using evaporation cracking and scouring tests. The hydrophysical properties and cohesive aggregation mechanism of biogel-amended clay were explored, and the results showed that the incorporation of biogel improved the water retention, cracking resistance, and scour resistance of the clay samples. With an increase in the biogel content, the biogel mucous membrane inside the samples improved the cohesion between soil particles, reduced the generation and development of cracks, and improved the cracking resistance. There was no significant cracking of the samples after the biogel content reached 0.3%, which changed the migration of water in the sample, prevented water evaporation, and improved the water retention of the clay samples. Biofilm can change the migration of water in the sample, prevent some evaporation, and reduce the evaporation rate. To a certain extent, it can enhance the water retention capacity of the sample. Enhanced biofilm content significantly reduced scouring in the process of rainfall and runoff erosion of the sample, and biofilm content of 0.2% significantly reduced the surface of the specimen damaged by erosion. The hydrophysical properties of the composite-adhesive-amended clay samples were significantly improved compared with those of the single-bioadhesive-amended clay samples.
Highly Stable, Mechanically Enhanced, and Easy-to-Collect Sodium Alginate/NZVI-rGO Gel Beads for Efficient Removal of Cr(VI)
Authors: Jing Qi , Ma Yuheng , He Jingwen , Ren Zhongyu
Published: September 2023

Nanoscale zero-valent iron (NZVI) is a material that is extensively applied for water pollution treatment, but its poor dispersibility, easy oxidation, and inconvenient collection limit its application. To overcome these drawbacks and limit secondary contamination of nanomaterials, we confine NZVI supported by reduced graphene oxide (rGO) in the scaffold of sodium alginate (SA) gel beads (SA/NZVI-rGO). Scanning electron microscopy showed that the NZVI was uniformly dispersed in the gel beads. Fourier transform infrared spectroscopy demonstrated that the hydrogen bonding and conjugation between SA and rGO allowed the NZVI-rGO to be successfully embedded in SA. Furthermore, the mechanical strength, swelling resistance, and Cr(VI) removal capacity of SA/NZVI-rGO were enhanced by optimizing the ratio of NZVI and rGO. Interestingly, cation exchange may drive Cr(VI) removal above 82% over a wide pH range. In the complex environment of actual Cr(VI) wastewater, Cr(VI) removal efficiency still reached 70.25%. Pseudo-first-order kinetics and Langmuir adsorption isotherm are preferred to explain the removal process. The mechanism of Cr(VI) removal by SA/NZVI-rGO is dominated by reduction and adsorption. The sustainable removal of Cr(VI) by packed columns could be well fitted by the Thomas, Adams–Bohart, and Yoon–Nelson models, and importantly, the gel beads maintained integrity during the prolonged removal. These results will contribute significant insights into the practical application of SA/NZVI-rGO beads for the Cr(VI) removal in aqueous environments.
Effects of Grafting Degree on the Formation of Waterborne Polyurethane-Acrylate Film with Hard Core–Soft Shell Structure
Authors: Kwon Yong Rok , Moon Seok Kyu , Kim Hae Chan , Kim Jung Soo , Kwon Miyeon , Kim Dong Hyun
Published: September 2023

Waterborne polyurethane-acrylate (WPUA) grafted with polyurethane was prepared to improve the film-forming ability of hard-type acrylic latex. To balance the film-formation ability and hardness, the WPUA latex was designed with a hard core (polyacrylate) and soft shell (polyurethane). The grafting ratio was controlled through varying the content of 2-hydroxyethyl methacrylate (HEMA) used to cap the ends of the polyurethane prepolymer. The morphologies of the latex particles, film surface, and fracture surface of the film were characterized through transmission electron microscopy, atomic force microscopy, and scanning electron microscopy, respectively. An increase in the grafting ratio resulted in the enhanced miscibility of polyurethane and polyacrylate but reduced adhesion between particles and increased minimum film formation temperature. In addition, grafting was essential to obtain transparent WPUA films. Excessive grafting induced defects such as micropores within the film, leading to the decreased hardness and adhesive strength of the film. The optimal HEMA content for the preparation of a WPUA coating with excellent film-forming ability and high hardness in ambient conditions was noted to be 50%. The final WPUA film was prepared without coalescence agents that generate volatile organic compounds.
Copolymeric Micelles of Poly(ε-caprolactone) and Poly(methacrylic acid) as Carriers for the Oral Delivery of Resveratrol
Authors: Kamenova Katya , Radeva Lyubomira , Konstantinov Spiro , Petrov Petar D. , Yoncheva Krassimira
Published: September 2023

In this study, we report the development of a micellar system based on a poly(methacrylic acid)-b-poly(ε-caprolactone)-b-poly(methacrylic acid) triblock copolymer (PMAA16-b-PCL35-b-PMAA16) for the oral delivery of resveratrol. The micellar nanocarriers were designed to comprise a PCL core for solubilizing the poorly water-soluble drug and a hydrated PMAA corona with bioadhesive properties for providing better contact with the gastrointestinal mucosa. The micelles were first formed in an aqueous media via the solvent evaporation method and then loaded with resveratrol (72% encapsulation efficiency). Studies by transmission electron microscopy (TEM) and dynamic and electrophoretic light scattering (DLS and PALS) revealed a spherical shape, nanoscopic size (100 nm) and a negative surface charge (−30 mV) of the nanocarriers. Loading of the drug slightly decreased the hydrodynamic diameter (Dh) and increased the ƺ-potential of micelles. In vitro dissolution tests showed that 80% and 100% of resveratrol were released in 24 h in buffers with pH 1.2 and 6.8, respectively, whereas for the same time, not more than 10% of pure resveratrol was dissolved. A heat-induced albumin denaturation assay demonstrated the advantage of the aqueous micellar formulation of resveratrol, which possessed anti-inflammatory potential as high as that of the pure drug. Further, the micellar resveratrol (5 µM) exerted a strong protective effect and maintained viability of mucosa epithelial HT-29 cells in a co-cultural model, representing the production of inflammatory cytokines. For comparison, the pure resveratrol at the same concentration did not protect the damaged HT-29 cells at all. Thus, the present study revealed that the PMAA-b-PCL-b-PMAA copolymeric micelles might be considered appropriate nanocarriers for the oral delivery of resveratrol.
Chitosan Coatings Modified with Nanostructured ZnO for the Preservation of Strawberries
Authors: García-García Dulce J. , Pérez-Sánchez G. F. , Hernández-Cocoletzi H. , Sánchez-Arzubide M. G. , Luna-Guevara M. L. , Rubio-Rosas E. , Krishnamoorthy Rambabu , Morán-Raya C.
Published: September 2023

Strawberries are highly consumed around the world; however, the post-harvest shelf life is a market challenge to mitigate. It is necessary to guarantee the taste, color, and nutritional value of the fruit for a prolonged period of time. In this work, a nanocoating based on chitosan and ZnO nanoparticles for the preservation of strawberries was developed and examined. The chitosan was obtained from residual shrimp skeletons using the chemical method, and the ZnO nanoparticles were synthesized by the close-spaced sublimation method. X-ray diffraction, scanning electron microscopy, electron dispersion analysis, transmission electron microscopy, and infrared spectroscopy were used to characterize the hybrid coating. The spaghetti-like ZnO nanoparticles presented the typical wurtzite structure, which was uniformly distributed into the chitosan matrix, as observed by the elemental mapping. Measurements of color, texture, pH, titratable acidity, humidity content, and microbiological tests were performed for the strawberries coated with the Chitosan/ZnO hybrid coating, which was uniformly impregnated on the strawberries’ surface. After eight days of storage, the fruit maintained a fresh appearance. The microbial load was reduced because of the synergistic effect between chitosan and ZnO nanoparticles. Global results confirm that coated strawberries are suitable for human consumption.
Effect of Palmitic Acid on Tertiary Structure of Glycated Human Serum Albumin
Authors: Szkudlarek Agnieszka
Published: September 2023

Non-enzymatic glycation is a process, which can be best described as a significant posttranslational modification of various proteins. It emerges in hyperglycemic conditions and may have an impact on albumin stability as well as its activity and physical and chemical properties, essentially affecting all its physiological functions. The goal of this research was to answer the following questions: (i) how does the glycation of defatted human serum albumin by glucose–fructose syrup (GFS) alter its tertiary structure; (ii) does palmitic acid (PA), a component of palm oil, affect the in vitro glycation process and cause conformational changes of glycated albumin; and (iii) does PA inhibit the formation of Advanced Glycation End-Products (AGEs)? Therefore, in order to point out differences in the tertiary structure of macromolecules, the absorption and emission of fluorescence spectra and their second derivatives, excitation fluorescence and synchronous spectra, Red-Edge Excitation Shift (REES effect), and the degree of modification of sulfhydryl groups of defatted, non-glycated (HSA) and glycated human serum albumin (gHSA) with GFS and glycated with GFS and PA were investigated. In the present study, it has been confirmed that the glycation of albumin in the presence of GFS and PA causes changes in both HSA and gHSA tertiary structures, respectively. Moreover, palmitic acid, at ratios of 1.5:1 and 3:1 with glycated albumin, does not exhibit inhibition of AGEs formation. This study indicates the fact that the structural changes, especially those of glycated albumin, are important for treatment planning because the type of the interaction between the components and their primary transporter may be altered as the disease progresses or in the elderly.
Evaluation of Supramolecular Gel Properties and Its Application in Drilling Fluid Plugging
Authors: Du Xiaoyong , Feng Shaobo , Lu Haiying , Bai Yingrui , Lv Zhiqiang
Published: September 2023

Supramolecular gels are physically cross-linked hydrogels formed by non-covalent interactions. The synthesis, structure optimization, property regulation, and application expansion of supramolecular gels has gradually become the research hotspot in the field of gel materials. According to the non-covalent interactions such as hydrophobic association and hydrogen bonding, the supramolecular gel prepared in this study has excellent rheological properties and adaptive filling and plugging properties, and can be used in the field of drilling fluid plugging. In this paper, the microstructure, rheological properties, temperature resistance, and plugging properties of supramolecular gels were studied and characterized in detail. The experimental findings demonstrated that when the strain was less than 10%, the supramolecular gel displayed an excellent linear viscoelastic region. The increase in strain weakens the rheological properties of supramolecular gel and reduces the elastic modulus of supramolecular gel to a certain extent. The supramolecular gel still had a neat three-dimensional reticular structure after curing at high temperatures, and the network of each layer was closely connected. Its extensibility and tensile properties were good, and it had excellent temperature resistance and mechanical strength. The supramolecular gel had excellent tensile and compressive properties and good deformation recovery properties. When the elongation of the supramolecular gel reached 300%, the tensile stress was 2.33 MPa. When the compression ratio of supramolecular gel was 91.2%, the compressive stress could reach 4.78 MPa. The supramolecular gel could show an excellent plugging effect on complex loss layers with different fracture pore sizes, the plugging success rate could reach more than 90%, and the plugging layer could withstand 6.3 MPa external pressure. The smart plugging fluid prepared with supramolecular gel material could quickly form a fine barrier layer on the rock surface of the reservoir. It could effectively isolate drilling fluid from entering the reservoir and reduce the adverse effects, such as permeability reduction caused by drilling fluid entering the reservoir, so as to achieve the purpose of reservoir protection.
Investigating the Microwave-Assisted Extraction Conditions and Antioxidative and Anti-Inflammatory Capacities of Symphytum officinale WL Leaves
Authors: Lou Kuo-Hao , Tsai Ming-Shiun , Wu Jane-Yii
Published: September 2023

Symphytum officinale (comfrey) is a perennial herb native to West Asia and Europe. Its root extracts are commonly used as a natural remedy to treat muscle, joint, skin, and bone disorders, especially in Europe. However, more information is needed on the biomedical functions of comfrey leaves. This study’s sequencing results of internal transcribed spacer and trnL–trnF genes showed that plants purchased from the local market were comfrey and named S. officinale WL (WL). The suitable extraction conditions of the WL leaves with the highest extract yield and total phenols and flavonoid contents by microwave-assisted extraction were identified. The antioxidative and anti-inflammatory activities and possible molecular mechanism(s) of the WL leaf extract (WLE) were evaluated. Furthermore, the major component of WLE was identified as rosmarinic acid by HPLC. Results showed that the optimal extract condition was obtained with 750 W microwave power, 50 °C, 75% methanol, the solid-to-solvent ratio of 1:10, and 15 min. Results of all DPPH, ABTS, and superoxide radical scavenging activities, reducing power, ferrous ion chelating activity, and ferric reducing antioxidant power showed high antioxidative capacities of WLE. Furthermore, WLE showed prominent DNA-protecting activity. WLE attenuated lipopolysaccharide-stimulated inflammation by suppressing iNOS, COX-2, IL-1β, IL-6, and TNF-α expressions in the RAW264.7 macrophages. These attenuations are involved in the inactivation of lipopolysaccharide-stimulated NF-κB and MAPK signaling pathways. Therefore, the comfrey leaf extract obtained via a time- and energy-saving microwave-assisted extraction may be a potential antioxidative and anti-inflammatory biomedicinal agent.
Rumex crispus Leaf Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in BV-2 Microglia Cells
Authors: Park Ji-Woong , Kim Woong , Choi Chul Yung , Kim Seok-Jun
Published: September 2023

Background: Microglial cells are immune cells that operate within the central nervous system. Abnormally activated microglia cause neuroinflammation, which is linked with neurodegenerative disease. Previous research has revealed that Rumex crispus root extract exerts anti-inflammatory effects. However, it is not known whether Rumex crispus leaf extract (RLE) has anti-inflammatory effects on murine microglial cells, such as BV-2 cells. This study proposed to investigate the impact of RLE on inducing inflammation by LPS in BV-2 cells. Methods: LPS was used to induce inflammation in BV-2 cells, and then cell survival, changes in the levels of inflammation-related factors and pro-inflammatory cytokines, and NF-κB and MAPKs signaling pathway activity were evaluated in the presence or absence of RLE. Results: RLE treatment resulted in a reduction in nitric oxide (NO) production triggered by LPS without causing cytotoxic effects. In addition, both protein and mRNA expression levels of iNOS and COX-2, which were upregulated by LPS, were significantly decreased by RLE. Also, RLE effectively reduced the transcriptional expression and further suppressed the increased production of inflammatory cytokines by LPS stimulation. Additionally, RLE effectively suppressed the inflammatory response of BV-2 cells stimulated by LPS via interference with NF-κB and MAPK signaling pathways. Conclusions: Taken together, our results confirm the effective suppression of the inflammatory response induced by LPS in BV-2 cells by RLE. Consequently, we suggest that RLE holds promise as a preventive agent against diseases triggered by microglial inflammatory responses.
A Great Camp Meeting: Meditating on the Black Faiths of Our Children
Authors: Davis Corwin Malcolm
Published: September 2023

The antiblack violences of this world are harsh and unrelenting, and the assaults are gratuitous. It remains a difficult task for many Black adults to get out of bed every day and to face both the expected and the unforeseen horrors of the day. How much more might this be true for our children? And how might they already be leaning into their own formations of spiritual action in response? Taking seriously, then, how Black children utilize imagination, creativity, and play, I use this meditation to think alongside children as epistemological sources for Black faith, and I turn to questions about how these spiritual technologies might be read as practices of conjure.
Positioning Chaplaincy in the Pluralistic and Multidisciplinary Dutch Care Context
Authors: Visser Anja , Zock Hetty , Muthert Hanneke
Published: September 2023

The professional identity of chaplains is under question because of societal trends of disaffiliation from and pluralization of religion, and of deinstitutionalization of care. Chaplaincy in the Netherlands looks to discourse around “meaning” to navigate these challenges. The use of the term “meaning” as the central concept in the professional identity of chaplaincy (and, by extension, spiritual care) is not undisputed, however. There are three related critiques: 1. Meaning and meaning-making have a strong cognitive and intentional connotation, which does not do justice to the lived experience of meaning and might lead to a medicalization of meaning. 2. The term meaning places the professional identity of chaplaincy in the instrumental discourse of other professions, which might lead to “abuse” of spiritual care toward external objectives such as health, (hedonistic) well-being, and/or economic gain, instead of internal objectives such as faith and spirituality. 3. A focus on meaning leads to a marginalization of religion, both societally and within chaplaincy, which might negatively affect chaplaincy’s core competence of hermeneutic understanding and worldview counseling. We conclude that finding one language to present the discipline might not be feasible and desirable. Instead, we advocate for the revitalization of the hermeneutic competency of chaplains.
The Benefits of Music in Teaching Catholic Religious Education in Croatia
Authors: Garmaz Jadranka , Baučić Sara Dodig
Published: September 2023

Music viewed from the real praxis could trigger positive religious emotions as the path of beauty in those searching for the meaning of life or the higher one in God. The article aims to encourage interdisciplinarity in teaching Catholic RE in Croatia, presenting many benefits of music and examples of positive practices that music can encourage in students. The main purpose of this paper, after recognizing the needs and difficulties of interdisciplinary teaching primarily for teachers in Croatia, is to create a model of implementing music content efficiently in Catholic religious education (RE) in Croatia. The research finds the necessity to unite experts from three fields to make interdisciplinary teaching efficient and progressively constructive: religious education pedagogy and music pedagogy combined with music practice itself through professional conductors. Nevertheless, the text seeks to expose an easily applicable model that leads to efficient and progressive interdisciplinary teaching, which is also the main aim of the work. The research is significant as this model could be examined in different subject areas, highlighting that singing in class could encourage religious sensibility and help improve spiritual and religious competencies. The research findings showed that the advantages and specifics of choral singing could be integrated into the religious education curriculum, making RE more dynamic and challenging for students. The research is based on a literature review, a data analysis, as well as participant observation (teacher and conductor) as methods to prove advantages and possibilities of how to implement music into the RE curriculum.