bluuebunny/crossref_metadata_2025 huggingface dataset:
Unfolded Protein Response is Involved in Trans-Platinum (II) Complex-Induced Apoptosis in Prostate Cancer Cells via ROS Accumulation
Authors: Karakas Didem , Cevatemre Buse , Oral Arzu Y. , Yilmaz Veysel T. , Ulukaya Engin
Published: April 2019

Background:

Prostate cancer is one of the most common cancer types and it is the sixth leading cause of cancer-related death in men worldwide. Even though novel treatment modalities have been developed, it still a lifethreatening disease. Therefore novel compounds are needed to improve the overall survival.

Methods:

In our study, it was aimed to evaluate the anti-cancer activity of newly synthesized Platinum (II) [Pt(II)] complex on DU145, LNCaP and PC-3 prostate cancer cell lines. The cytotoxic activity of Pt(II) complex was tested by SRB and ATP cell viability assays. To detect the mode of cell death; fluorescent staining, flow cytometry and western blot analyses were performed.

Results:

The Pt(II) complex treatment resulted in a decrease in cell viability and increasing levels of apoptotic markers (pyknotic nuclei, annexin-V, caspase 3/7 activity) and a decrease in mitochondrial membrane potential in a dose dependent manner. Among cell types, tested PC-3 cells were found to be more sensitive to Pt(II) complex, demonstrating elevation of DNA damage in this cell line. In addition, Pt(II) complex induced Endoplasmic Reticulum (ER) stress by triggering ROS generation. More importantly, pre-treatment with NAC alleviated Pt(II) complex-mediated ER stress and cell death in PC-3.

Conclusion:

These findings suggest an upstream role of ROS production in Pt(II) complex-induced ER stressmediated apoptotic cell death. Considering the ROS-mediated apoptosis inducing the effect of Pt(II) complex, it warrants further evaluation as a novel metal-containing anticancer drug candidate.
“Proposals for Amendments in the Diagnosis and Treatment of Encephalitis caused by Free-living Amoebae”
Authors: Baig Abdul Mannan
Published: April 2019

Encephalitis caused by Free-living amoebae (FLA) has a mortality rate of around 95-
98%, a fraction that has not changed in the past decades. Pathogenic FLA include Acanthamoeba,
Balamuthia mandrillaris, and Naegleria fowleri that are known to target the brain after an extra
cerebral infection in the case of Acanthamoeba and Balamuthia mandrillaris, or directly the brain,
as in the case of the Naegleria fowleri. The Acanthamoeba spp. and Balamuthia mandrillaris
cause granulomatous amoebic encephalitis (GAE) while Naegleria fowleri, the so termed “brain
eating amoeba” causes primary amoebic meningoencephalitis (PAM). The attempts to obtain a
speedy diagnosis and an aggressive treatment protocol are the areas where advances can make a
difference and reduce the mortality rates. At first, we highlight the reasons behind the diagnostic
delays and treatment failures and provide proposals to establish a quick diagnosis in both PAM
and GAE. Secondly, we emphasize the use of a transcribrial device, and a prompt, but vigilant
surgical reduction of the intracranial pressure in these patients which could be life-saving. We also
debate that an exudate obtained from the olfactory region by irrigation via a modified transcribrial
device or by conventional methods, instead of a cerebrospinal fluid sample, could serve as a
source of obtaining amoeba in PAM for a real-time polymerase chain reaction-based definitive diagnosis
of PAM. Also, introduced is the rationale that has the potential to deliver the drugs to the
brain in patients with PAM and the GAE localized to the frontal lobe of the brain, by bypassing
the blood brain barrier. We put forward these proposals for debate and deliberation to our fellow
colleagues in order to spot the potential of their application to reduce the mortality rates caused by
the rare but fatal encephalitis caused by these FLA.
Characteristic Performance of OLED Based on Hole Injection, Transport and Blocking Layers
Authors: Negi Shubham , Mittal Poornima , Kumar Brijesh
Published: April 2019

Background:

Organic Light Emitting Diodes have emerged as a potential candidate for
being used as the display because of their remarkable performance. In the past decade, a lot of interest
was shown in their development and improvement. Scientists utilized different methods for
enhancing their performance which included the change in OLEDs architecture. Different supporting
layers (charge injection, transportation and blocking) are added in the architecture to enhance
the performance of OLED. Some of the patents related to the application of the OLED have also
been reviewed to observe the utilization of the present work in those applications.

Objective:

The motive of this research article is to analyze impacts the impact of different layers on
the performance of OLED.

Methods:

The objective is achieved by systematically analyzing the impact of different layers on
the OLED. The analysis commenced by investigating the properties of the basic OLED consisting
of the emission layer only. Thereafter, in succession different supporting layers are added to the
OLED architecture. Subsequently, after adding each layer, OLED device is analyzed and its properties
are determined. Finally, the performance of different layers is compared.

Results:

The outcome of the above research study shows that these supporting layers are significantly
able to enhance the performance of the OLED. Step by step layers are added to the architecture
and enhancement in the performance is observed. There is an improvement of 13% to 38% not
over the basic OLED architecture, but over the multilayered OLED device, which is already far
better than the basic OLED.

Conclusion:

Therefore, from the analyses, it can be concluded that the different layers are significant
for the OLED architecture and improve the performance of the OLED considerably.
Multiple Evolutionary Mechanisms Reduce Protein Aggregation
Authors: Reumers Joke , Rousseau Frederic , Schymkowitz Joost
Published: April 2019

The folding of polypeptides into stable globular protein structures requires protein sequences with a relatively
high hydrophobicity and secondary structure propensity. These biophysical properties, however, also favor protein
aggregation via the formation of intermolecular beta-sheets and, as a result, globular structure and aggregation are
inextricable properties of protein polypeptides. Aggregates that are enriched in beta-sheet structures have been found in
diseased tissues in association with at least twenty different human disorders and the effect of aggregation on protein
function include simple loss-of-function but also often a gain of toxicity. Given both the ubiquity and the potentially lethal
consequences of protein aggregation, negative selective pressure strongly minimizes aggregation. Various evolutionary
strategies keep aggregation in check, including (1) the optimisation of the thermodynamic stability of the protein, which
precludes aggregation by burial of the aggregation prone regions in solvent inaccessible regions of the structure, (2)
segregation between folding nuclei and aggregation nuclei within a protein sequence, (3) the placement of so-called
gatekeeper residues at the flanks of aggregating segments, that reduce the aggregation rate of (partially) unfolded proteins,
and (4) molecular chaperones that target aggregation nucleating sequences directly, thereby further suppressing
aggregation in a cellular environment. In this review we describe the intrinsic features built into protein sequence and
structure that protect against aggregation.
The Tightly Regulated and Compartmentalised Import, Sorting and Folding of Mitochondrial Proteins
Authors: Cassina Laura , Casari Giorgio
Published: April 2019

Mitochondria are eukaryotic intracellular organelles that still bear the signatures of their prokaryotic ancestor
and require nuclear assistance. They generously dispense energy to cells, but are also involved in several biosynthetic
processes, as well as in cell signalling pathways and programmed cell death.

Mitochondria are partitioned into four intra-organelle compartments: the outer membrane, the inner membrane, the
intermembrane space and the matrix. Each compartment contains a unique set of proteins and a personalised system for
guaranteeing protein homeostasis.

What follows is a survey of the function and topology of the multiple systems that operate the concerted action of protein
sorting and folding in the four mitochondrial compartments.
Perspectives for Early Genetic Screening of Lactose Intolerance: - 13910C/T Polymorphism Tracking in the MCM6 Gene
Authors: A.S. Arroyo Marta , P. Lopes Ana Cláudia , B. Piatto Vania , Victor Maniglia José
Published: April 2019

Introduction:

For many years Lactose intolerance has been, considered as a universal problem in many children
and adults.

Objective:

The aim is to investigate the prevalence of polymorphism -13910C/T, in a neonatal tracking, for
early diagnosis of lactose tolerance/intolerance. Materials and Methods: In a cross-sectional study of 310 Brazilian
newborns, DNA was extracted from leukocyte umbilical cord and specific primers were used to amplify the region that
encloses the -13910C/T polymorphism of the MCM6 gene, using the polymerase chain reaction and the restriction
fragment length polymorphism tests.

Results:

One hundred and sixty (52%) male newborns and 150 (48%) female new
borns were evaluated. Out of these, 191 (62%) presented CC genotype (lactose intolerant), 95 (31%) CT genotype, and 24
(7%) TT genotype, comprising a total of 119 (38%) lactose tolerant newborns. Accordingly the newborns´ gender
distribution in relation to the phenotypes has been found; 97 (32%) of male gender and 94 (30%) of female gender lactose
intolerant, and 63 (20%) male and 56 (18%) female lactose tolerant newborns, not being such distribution statistically
significant (p = 0.801).

Conclusions:

The molecular analysis made possible the identification of the presence or absence of
lactase persistence variant in the Brazilian newborns. The neonatal molecular diagnosis can optimize the follow-up of
positive results in newborn screening for lactose intolerance.
Safety of Using Diapers Containing Copper Oxide in Chronic Care Elderly Patients
Authors: I Weinberg , A Lazary , A Jefidoff , J-J Vatine , G Borkow , N Ohana
Published: April 2019

Copper has very potent antibacterial, antifungal, antiviral and acaricidal properties. Recently the use of copper
alloys in hospital wards has been shown to reduce bioburden and nosocomial infection rates. We hypothesized that the use
of copper oxide in textiles and other products that are in close contact with the patients may significantly reduce
bioburden in clinical settings and consequently reduce the risk of nosocomial infections. In order to test this hypothesis we
intend to conduct a trial in which we will examine if the nosocomial infection rates in a chronic care ward will be reduced
when all the textile products will include copper oxide. The risk of adverse reactions due to dermal contact with copper
are considered extremely low and medical devices containing copper, such as intrauterine devices and dental amalgams,
are safely used for decades. Textile products containing copper oxide are being sold worldwide for several years and not
even one adverse reaction was noted. However, in spite of all the above, and in preparation to the clinical trial we intend
to conduct in a chronic care ward (long-term care facility), we conducted this preliminary study in which we examined the
safety of using copper oxide impregnated diapers in 16 chronic care patients that used the diapers for 6 consecutive
months. Importantly, not even one adverse reaction was recorded during the whole trial, indicating the high safety of the
diapers. Therefore, this study allows us to examine the efficacy of textiles containing copper oxide in reducing nosocomial
infections in larger populations, including in frail chronic care patients.
Performance Analysis of Efficient and Low Complexity MIMO-OFDM System Using Modified V-BLAST
Authors: Yadav Brijesh Kumar , Singh Rabindra Kumar
Published: April 2019

Background and Objective:

To analyze the performance of MIMO-OFDM system,
VBLAST detection suffers from computational complexity as well as high BER.

Methods:

To improve the BER performance and to reduce the computational complexity of receiver,
we propose a modified VBLAST detection in MIMO-OFDM Systems.

Results:

Simulation using MATLAB shows the improvement in computational complexity and in BER.

Conclusion:

This modified VBLAST reduces the complexity of MIMO-OFDM System to 44.2% and
improvement in BER as compared to conventional VBLAST based MIMO-OFDM System.
Nanocrystalization: An Emerging Technology to Enhance the Bioavailability of Poorly Soluble Drugs
Authors: Joshi Kavita , Chandra Akhilesh , Jain Keerti , Talegaonkar Sushama
Published: April 2019

Most of the active pharmaceutical ingredient used in the management of disease
have poor water solubility and offer grueling problems in drug formulation development
since low solubility is generally associated with poor dissolution characteristics which
leads to poor oral bioavailability. The great challenge for the development of a pharmaceutical
product is to create its new formulation and drug delivery system to limit solubility
problems of existing drug candidate. Limited drug-loading capacity requires a large
amount of carrier material to get appropriate encapsulation of the drug, which is another
major challenge in the development of pharmaceutical product which could be resolved by
developing nanocrystals (NCs). A significant research in the past few years has been done
to develop NCs which helps in the delivery of poorly water soluble drugs via different
routes. The technology could continue to thrive as a useful tool in pharmaceutical sciences
for the improvement of drug solubility, absorption and bioavailability. Many crystalline
compounds have pulled in incredible consideration much of the time, due to their ability to
show good physical and chemical properties when contrasted with their amorphous counterparts.
Nanocrystals have been proven to show atypical properties compared to the bulk.
This review article explores the principles of the important nanocrystallization techniques
including NCs characterization and its application.
Cocoa Pulp as Alternative Food Matrix for Probiotic Delivery
Authors: Guimarães Gabriele M. , Soares Letícia A. , Silva Tuânia N.L. , de Carvalho Iracema L.Q. , Valadares Helder M.S. , Sodré George A. , Gonçalves Daniel B. , Neumann Elisabeth , Da Fonseca Flávio G. , Vinderola Gabriel , Granjeiro Paulo A. , de Magalhães Juliana T.
Published: April 2019

Background:

For screening probiotic strains with viability and stability in non-dairy
foods for health benefits, we revised all patents relating to probiotics in food.

Objective:

Screening of potential probiotics from Brazilian Minas artisanal cheese and verify their
survival in frozen Brazilian cocoa pulp.

Methods:

Isolation and identification of the strains. The potential probiotic characterization involved
gastric juice and bile resistance, antibiotic and antimicrobial activity, hydrophobicity,
autoaggregation, coaggregation and adhesion assay in HT-29 cells. Organoleptic, viability and stability
of probiotic strain in frozen cocoa pulp were evaluated.

Results:

Fourteen strains of Lactobacillus plantarum (9), Weissella paramesenteroides (3), Lactobacillus
fermentum (1), and Leuconostoc mesenteroides (1) were obtained. Most of the strains were
resistant to simulated gastric acidity and bile salts. Almost all strains were sensitive to the antibiotics
tested, except to ciprofloxacin and vancomycin. About 47% of the strains are potential producers
of bacteriocins. High hydrophobicity was observed for four strains. Autoaggregation ranged
from 8.3-72.6% and the coaggregation capacity from 5.2-60.2%. All of the assessed strains presented
more than 90% of adhesion to HT-29 intestinal cells. The percentage of Salmonella inhibition
in HT-29 cells ranged from 4.7-31.1%. No changes in color, aroma, and pH were observed in
cocoa pulps after storage at -20 °C for 90 days.

Conclusion:

Wild strains of acid lactic bacteria from cheese proved to be viable and stable in frozen
Brazilian cocoa pulp. This work showed a promising application of L. plantarum isolated
strains to be used with frozen cocoa pulp matrix in probiotics food industry.
The effectiveness of teaching program of CIPP evaluation model
Authors: Darma I Ketut
Published: April 2019

This study aimed at finding out the level of effectiveness of the implementation of the teaching at the  Department of  Mechanical Engineering Politeknik Negeri Bali (PNB) viewed from  the interrelatedness of the components of context, input, process, and product, This study used the evaluative study model of CIPP (Context, Input, Process, Product) and was done in two stages. The data were collected using a questionnaire, interview guide, and related documents that have proved to be highly valid and reliable. The data were analyzed descriptive-qualitatively and descriptive-quantitatively. The result showed that the implementation of the teaching program at the Department of Mechanical Engineering PNB viewed from the interrelatedness of the components of CIPP falls into the fairly effective category. On the other hand, the constraints encountered in the teaching program implementation were faced in the components of context and product. The greatest constraints were found in the variables of process and product related to the evaluation of nonacademic learning achievement.
Remotely Monitored Gamification and Social Incentives to Improve Glycemic Control Among Adults With Uncontrolled Type 2 Diabetes (iDiabetes): Protocol for a Randomized Controlled Trial (Preprint)
Authors: Fortunato Michael , Harrison Joseph , Oon Ai Leen , Small Dylan , Hilbert Victoria , Rareshide Charles , Patel Mitesh
Published: April 2019

BACKGROUND

Type 2 diabetes is a significant cause of morbidity and mortality in the United States. Lifestyle modifications including increasing physical activity and losing weight have been demonstrated to improve glycemic control. However, most patients struggle to make these changes. Many stakeholders are interested in using gamification and social incentives to increase engagement in healthy behaviors. However, these approaches often do not appropriately leverage insights from behavioral economics that could be used to address predictable barriers to behavior change.

OBJECTIVE

This study aimed to describe the protocol for the Influencing DIabetics to Adapt Behaviors related to Exercise and weighT by Enhancing Social incentives (iDiabetes) trial, which aimed to evaluate the effectiveness of gamification interventions that leverage insights from behavioral economics to enhance supportive, competitive, or collaborative social incentives to improve glycemic control, promote weight loss, and increase physical activity among overweight and obese adults with type 2 diabetes.

METHODS

We are conducting a one-year four-arm randomized controlled trial of 361 overweight and obese patients with type 2 diabetes and a glycated hemoglobin (HbA1c) level ≥8.0. Wireless weight scales and wearable devices are provided to remotely monitor weight and physical activity and transmit data to the study team. Patients are recruited by email, following which they establish a baseline measure of weight, daily step count, HbA1c level, and low-density lipoprotein cholesterol level and then repeat these measures at 6 and 12 months. The control arm receives no other interventions. Patients randomized to one of the three intervention arms are entered into a game designed using insights from behavioral economics to enhance supportive, competitive, or collaborative social incentives. To examine predictors of strong or poor performance, participants completed validated questionnaires on a range of areas including their personality, risk preferences, and social network.

RESULTS

Enrollment of 361 patients was completed in January 2019. Results are expected in 2020.

CONCLUSIONS

The iDiabetes trial represents a scalable model to remotely monitor the daily health behaviors of adults with type 2 diabetes. Results from this trial will help provide insights into how to improve management of patients with type 2 diabetes.

CLINICALTRIAL

ClinicalTrials.gov NCT02961192; https://clinicaltrials.gov/ct2/show/NCT02961192
Modelos educativos de postgrado: teoría y ejecución
Authors: Núñez Méndez Maribel
Published: April 2019

Este artículo presenta un estudio exploratorio realizado en el nivel de postgrado de una universidad de Santo Do­mingo, República Dominicana, donde se estudió el uso de los modelos educativos reflejados en la metodología de enseñanza de los docentes del cuarto nivel. Se abordó el manejo e implementación de ciertas técnicas y estrategias utilizadas por los docentes y la relación observable entre la perspectiva docente y la literatura experta. Para reali­zar esta investigación, se utilizó una metodología donde se prioriza la indagación, con un enfoque descriptivo de profundización, para explicar lo que se plantea sobre las diversas estrategias metodológicas existentes y la manera en que los docentes apropian e implementan las teorías, a través de estas. Se concluye que no todos los docentes em­plean métodos adecuados y motivadores, que sean propios del nivel, del estudiantado y del plan de estudio. Al final se ofrecen algunas recomendaciones.
Interprofessional Learning for Enhanced Patient Safety: Biomedical Engineering Students and Nursing Students in Joint Learning Activities
Authors: Mattsson Janet , Ostlund Britt , Bjorling Gunilla , Williamsson Anna , Eriksson Andrea
Published: April 2019

Background: In the last decade, research has highlighted the importance of interprofessional approaches to education and practice. Collaboration between medical practice and engineering has been identified as particularly relevant to developing accountable models for sustainable healthcare and overcoming increased specialization leading to professional barriers. This study aims to analyze insights and understanding expressed by nursing students and biomedical engineering students following a joint learning activity regarding a medical device used in the hospital setting.Method: A qualitative approach deriving from a phenomenological view examined an interprofessional learning activity where the focus was on active integration and knowledge exchange.Conclusion: The activity was expressed as a positive opportunity for getting insights into perspectives from other professional groups as well as insights into the importance of a system perspective in patient safety. The learning and insights listed in the evaluations included ideas about how the two professional groups could collaborate in the future.
GRENALIZAÇÃO NA COPA DO MUNDO: POLÍTICA IDENTITÁRIA NA ESCOLHA DA ARENA PORTO-ALEGRENSE
Authors: Leão André Luiz Maranhão de Souza , Ferreira Bruno Rafael Torres , Gomes Victor Pessôa de Mélo
Published: April 2019

A escolha do estádio-sede de Porto Alegre para a Copa do Mundo se deparou com uma das maiores rivalidades clubísticas do país, a qual levou à criação do termo grenalização, que designa como esta polarização adentra espaços além do futebol, o que articulamos como uma política identitária. O Beira-Rio foi escolhido, mas não sem polêmica e a insistência, do lado gremista, de que a Arena do Grêmio teria um projeto adequado e de que o Internacional teria sido privilegiado. O presente trabalho teve por objetivo avaliar os argumentos presentes nos discursos em torno das novas arenas. Para tal, acessamos três posições discursivas sobre o assunto: dos organizadores, da imprensa e da sociedade. Os dados foram coletados por meio de entrevistas, observações diretas e documentos. O método usado foi a Análise de Discurso Foucaultiana. Como lente teórica, adotamos a Teoria do Discurso de Laclau e Mouffe. Nossos achados apontam para três formações discursivas: uma sobre a grenalização no contexto da escolha do estádio e mais uma relativa a cada posição antagônica. Concluímos que o discurso colorado conseguiu se estabelecer como hegemônico, por ter assumido um significante vazio que localizou a polêmica em torno da discussão de aspectos técnicos da construção das arenas, o que contribuiu com a legitimação da escolha do Beira-Rio.
CHEGOU A HORA? O PROCESSO DE SUCESSÃO DE UMA PEQUENA EMPRESA FAMILIAR
Authors: Zonatto Patrinês Aparecida França , Sbissa Amanda Paterno , Teston Sayonara de Fátima , Zonatto Vinícius Costa da Silva
Published: April 2019

Este caso para ensino tem como objetivo analisar o processo de sucessão em uma pequena empresa familiar, a Werner Cia Ltda. Após 40 anos de empresa o Senhor Adroaldo se vê cansado, e acredita que está na hora de passar a empresa para os filhos administrarem. Ocorre, que o fundador se pergunta a todo momento: será que meus filhos estão prontos e aptos para administrarem a empresa? O Caso apresenta estratégias analisadas no processo sucessório adotadas por uma pequena empresa do ramo de vidraçaria. O caso para ensino pode ser utilizado como material didático em cursos de graduação e de pós-graduação em administração e áreas afins. A elaboração do texto seguiu uma metodologia apropriada para caso para ensino. Os aspectos apresentados têm por objetivo possibilitar a discussão de conceitos de Pequenas Empresas Familiares, o Processo Sucessório, Competências Empreendedoras e sua associação com a prática empresarial vivenciadas pelas empresas. Após a análise da situação apresentada, os alunos são convidados a discutir sobre o perfil do empreendedor, propondo estratégias, sob a ótica da teoria tridimensional, para que o processo sucessório ocorra de maneira satisfatória e não prejudique o crescimento e desenvolvimento da empresa. O caso é finalizado com notas de ensino e sugestões de aplicação em sala de aula.
SECONDARY DISEASES IN TANATOGENESIS IN HIV INFECTION
Authors: Koshevaya E. G. , Zinserling V. A.
Published: April 2019

The problem of HIV infection has not lost its relevance, despite the progress and deepening of our knowledge about the disease over the years of study. The paper reflects the detailed structure of lethal outcomes and secondary diseases in HIV infection, taking into account in-depth histological studies in three time periods (1996/97, 2006, 2016) on the basis of these protocols of pathoanatomical autopsies in S.P.Botkin. Some of the data obtained confirms the facts known from the literature, while others are new and need further in-depth study. An important aspect of the work was the assessment of the significance of individual diseases in the tanatogenesis of each case, taking into account all clinical, laboratory and morphological data. The range of HIV-associated diseases varied in different time periods, although in many cases it was insignificant. In general, during the 20-year period of time HIV infection has undergone significant pathomorphosis.
AUTOCHTHONOUS CASE OF CUTANEOUS LEISHMANIASIS IN HIV INFECTED PATIENT
Authors: Ermakova L. A. , Golovchenko N. V. , Nagorny S. A. , Pshenichnaya N. Yu. , Avanesova L. A. , Zhuravlev A. S.
Published: April 2019

Single cases of autochthonous visceral leishmaniasis are registered in the Crimea and Dagestan. The article presents the first case of autochthonous cutaneous leishmaniasis in an HIV infected resident of the Republic of Dagestan, where this disease had not previously been registered. The diagnosis was made on the basis of a parasitological study of material taken from an ulcer on the border between the upper lip and the right nostril. Examination of the patient revealed a high burden of HIV (4 473 014 copies/ml) and a low level of CD4+ (76×106/l; 9%), which indicated an advanced stage of HIV infection. The patient underwent antiretroviral therapy (treatment with amphotericin B), against which a good result was obtained. A decrease in viral load and an increase in the content of CD4 lymphocytes in the blood were noted. A scar has formed on the site of a leishmaniasis ulcer.
TO THE ASSESSMENT OF VERTICAL CRANE LOAD
Authors: Pichugin Sergii , Patenko Yulia
Published: April 2019

The problems of analytical and statistical modeling of the travelling cranes loads are carried out in the paper. The analytical model was expanded by the consideration of influence of the approach of cranes trolley, consideration of zero realization areas and crane impact zones which allowed to precise the statistical characteristics of the vertical crane loads. The numerical modeling which takes into account the accidental position of the crane bridge and position of the trolley on the crane bridge was used for the calculation the vertical crane loads from the four-wheel cranes. Comparison of the results showed that the design value of the vertical load per wheel is rarely realized. This fact should be used to clarify the crane load combination factors.
THE STUDY OF COMPLEX STRESS STATES OF ELEMENTS FILLING THE CELLS BETWEEN THE RIBS OF WOODEN LARGE-SPAN DOMES
Authors: Pyatikrestovsky Konstantin , Sokolov Boris
Published: April 2019

The article is devoted to the research of complex stress states of anisotropic materials under long-term loads. The article considers the design of criteria for short-term and long-term strengths proposed by G.A. Geniev in connection with the calculation of large-span structures of domes of laminated and solid wood, where the enclosure elements are included in the joint work with the power set in the form of meridional and circular ribs. In this case, the elements of an enclosure-plate are at a complex stress state and they work under compression with a shift. Joint work of such systems was studied by experiments on large-scale models and experimental construction. Large reserves of strength resistance are revealed. In particular, in 1985, the indoor skating rink at the stadium “Lokomotiv” was built as an experimental facility which implemented these conditions and it has been successfully operated for many years. However, there are some unsolved issues with the refinement of the actual operation of the structures and the development of the calculation algorithm. Some generalizations of the studied approaches are given.
RESTABILIZATION OF THE POSTCRITICAL EQUILIBRIUM OF ELASTIC SYSTEMS
Authors: Manuylov Gaik , Kosytsyn Sergey , Begichev Maxim
Published: April 2019

The application of the Appel-Vozlinsky theorem on the stability or instability conditions for bifurcation points of conservative elastic systems with a symmetric bifurcation diagram to evaluate restabilization possibility of structures under loads substantially larger than the first critical force. It is shown that restabilization is possible if the first eigenvalue of the Hesse matrix is a continuous alternating function of the load parameter, and the remaining eigenvalues are sign-definite quantities. The examples of the systems with restabilization are given: a high Mises girder and an elastic system composed of compressible rods.
SECOND DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF DOXYLAMINE SUCCINATE AND PYRIDOXINE HCL IN PHARMACEUTICAL FORMULATIONS
Authors: KUMAR KASTURI AMAR , RAJU MEDICHERLA KANAKA , YAGANTI RAGHUVEER , N. BALAKRISHNAN
Published: April 2019

Estimation of drugs in pharmaceutical formulations by using UV-Visible spectrophotometric method is one of the most frequently used techniques in pharmaceutical analysis. Simple and reliable second derivative spectrophotometric method was developed and validated for the simultaneous estimation of Doxylamine Succinate and Pyridoxine HCL in bulk and pharmaceutical formulations. The quantitative determination of the drugs was carried out using the second derivative values measured at 239 nm and 290 nm for Doxylamine Succinate and Pyridoxine Hcl respectively. The method has been developed and validated for the assay of Doxylamine Succinate and Pyridoxine HCL using methanol as diluents. Calibration graphs drawn at their wavelengths of determination were linear in the concentration range of 1-8 Âµg mL-1 for Doxylamine Succinate and 12.5 - 100 Âµg mL-1 for Pyridoxine Hcl. At 232 nm Doxylamine Succinate showed zero absorbance but Pyridoxine HCL had considerable amount of absorbance. Similarly at 290 nm Pyridoxine HCL showed zero absorbance but Doxylamine Succinate had considerable amount of absorbance. The limit of Quantification (LOQ) of Doxylamine Succinate and Pyridoxine HCL was found to be 0.6 Âµg mL-1 and 1Âµg mL-1 respectively and the limit of detection (LOD) was found to be 0.08 Âµg mL-1 and 0.3 Âµg mL-1 respectively. Comparison second derivative spectrum of Doxylamine Succinate and Pyridoxine HCL in standard and drug formulation solutions showed that the wavelength of maximum absorbance did not change. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. Developed second derivative spectrophotometric method was simple, accurate, precise, specific, sensitive and reproducible, which can be directly and easily applied to pharmaceutical dosage forms.
Vertical coordination in the Brazilian milk supply chain: the case of 3B Agro LTDA
Authors: Hayer Silas Simeon , Staduto Jefferson Andronio Ramundo , Darr Dietrich
Published: April 2019

The dairy business is characterized by generally low profit margins due to a very competitive market environment. Dairy farmers and milk processing companies, therefore, constantly seek to optimize their technical production processes in pursuit of further cost reductions, e.g. by means of improving herd management, milking and feeding practices. In addition, innovative business strategies may create competitive advantage in response to market conditions. While vertical coordination or even integration have been established successfully in most sectors of animal husbandry including poultry, pork and beef production, this strategy is mostly limited to supplying contracts and input support within the dairy sector. Various organizational models of increasing vertical coordination in dairy production exist, with great variation in terms of operational performance and profitability. Using the example of 3B Agro LTDA’s dairy production located in the municipality of Toledo, Brazil, the case study illustrates the significant efficiency potential that currently exists in the Brazilian dairy supply chain. The prevalence of short-term contractual commitments and an influential role of independent intermediaries give room to considerable opportunism of market participants, various principal-agent problems and severe issues regarding milk quality. The case study illustrates that, vertical coordination can increase control and efficiency along the supply chain. It also points out the importance of strong managerial competencies and capabilities of dairy farm managers in order to exploit fully the efficiency potential provided by vertical coordination.
Héléne Cixousun "Medusanın Gülüşü" Feminist Stilistik Analizi
Authors: Batular Fatma
Published: April 2019

Bu çalışmada, Feminist Biçembilimsel kuramı ışığında uygulanmaktadır Héléne Cixois’in The Laugh of The Medusa adlı eserinin dili incelenmiştir. Içerik analiz yöntemi kullanılarak incelenen makalede, temel olarak cinsiyet betimlemeleri ve metinin ima edilen ve altında yatan anlamları yorumlamak ve ortaya çıkartmak amaçlanmıştır. Héléne Cixous kullandığı dilde Medusa adlı eski Yunan mitolojisinden örnekler alıp, genç kadın dinleyicilerine önerilerde bulunmaktadır. Kadınların ataerkil toplumunun baskısı altında olduklarını ve bunlara nasıl meydan okuduklaını, ve bunun için nasıl savaştıklarını anlatmaktadır. Yazar eserinde genç kızları, kendilerini ifade etmeleri için cesaretlendiriyor. Ona göre genç kızlar, güçlü duygularını cesaretle kaleme dökmeli. Bununla beraber genç erkekler de kadının dünyasını anlamalıdırlar. Çalışma kısaca, dilin cinsiyetle ilgili özellikleri nasıl ortaya çıkardığıyla ilgilenmektedir.
Öğretmen Adaylarının Yabancı Dil Kaygısı
Authors: Öztaş Tüm Danyal
Published: April 2019

Son otuz yılda,yabancı dil kaygısı ve hedef dil performansı ile hedef dilde elde edilen başarılar arasında negatif korelasyon olduğunu gösteren kayda değer araştırmalar yapılmıştır. Bu kaygının sebepleri ve sonuçları derinlemesine incelenmiştir. Ancak, öğretmen yabancı dil kaygısı konusunda çok az araştırma yapılmıştır. Bu çalışma, yabancı dil kaygısı yüksek ana dili İngilizce olmayan öğretmen adaylarının yaşadığı dil kaygısının nedenlerini ve etkilerini araştırmaktadır. Bulgular, katılımcıların olumsuz değerlendirme korkusu ve hatalar konusundaki endişeleri nedeniyle kaygı duygularını yaşadıklarını göstermektedir. Dil kaygısının, öğretmenlerin hedef dili ve öğretmenlerin spontane ve aktif hedef dil kullanımı gerektiren sınıf etkinliklerini kullanma konusundaki isteksizliği gibi daha önce araştırılmamış yabancı dil eğitimi üzerinde bir takım olumsuz etkileri olduğu görülmektedir. Ayrıca, yabancı dil kaygısı yüksek olan öğretmen adayları kendi sınıfların kendi öğrencilerinin dil hatalarını düzeltme konusunda daha ısrarcı olduklarını söylemektedirler. Yabancı dil kaygısı düşük olan öğretmen adayları ise kendi sınıflarında kendi öğrencilerinin dil hataları üzerinde daha az durmakta ve daha çok öğrencilerini yabancı dilde iletişime geçme konusunda destekleyici bir tutum sergilediklerini söylemeketedirler. Bu makale öğretmen adaylarının potansiyel yabancı dil kaygısını aşmalarına yardımcı olmak için öneriler yapmakta, ayrıca öğretmenlerin yabancı dil kaygısına ilişkin gelecekteki çalışmaları yürütmek isteyen araştırmacılara yönelik tavsiyeler de yapmaktadır.
Sosyal Duygusal Öğrenme Çerçevesinde Öz Düzenleme Yabancı Dil Öğrenme Ölçeğinin
Authors: Zaimoğlu Senem
Published: April 2019

Sayısı her geçen gün biraz daha artan araştırmalar, yabancı dil öğreniminde öz-düzenlemenin önemini vurgulamaktadır (Dörnyei, 2005; Oxford, 2011; Chamot, 2014). Bu araştırmalar doğrultusunda, araştırmacılar, öğrencilerin dil öğrenimindeki zorluklarla başa çıkmalarına yardımcı olmak için çok sayıda araç geliştirmişlerdir. Ancak, bu araçlar genellikle sosyal ve duygusal öğrenme çerçevesinde değil, dil öğrenme stratejileri çerçevesinde tasarlanmıştır. Bu açıdan bakıldığında, bu çalışma, üniversite öğrencilerinin yabancı dil öğrenirken, sosyal ve duygusal öğrenme çerçevesinde öz-düzenleme yetkinliklerini belirlemek için uygun bir araç geliştirerek bu boşluğu doldurmaya çalışmaktadır. Ayrıca, cinsiyetin dil öğrenenlerinin öz-düzenleme yetkinlikleri üzerindeki etkileri de yapısal eşitlik modeli kullanılarak incelenmiştir. Ölçeklerin faktör yapısı ve iç tutarlılığıyla ilgili veriler üç farklı zamanda toplam olarak da bir üniversitenin 1439 hazırlık okulu öğrencisine uygulanarak toplanmıştır. Öz-Düzenleme Yabancı Dil Öğrenme Ölçeğinin (SRFLLS) temel bileşen analizi, sekiz faktörlü bir yapı ortaya koymuştur: Güçlü Yönlerin ve Duyguların Tanınması, Öz-yeterlilik, Duygusal Bedeller, Öz-Motivasyon, Öz Disiplin, Hedef Belirleme, Yardım Arama ve Merak. Cevapların doğrulayıcı faktör analizlerine göre ise, model uyum değerlerinin kabul edilebilir olduğu ve cinsiyete bağlı olarak dil öğrenenlerin Öz-disiplin, Yardım Arama ve Merak yeterliliklerinde anlamlı bir farklılık olduğu gözlemlenmiştir. Çalışma sınırlamaları ve gelecekteki araştırmalar için açıklamalar ele alınmıştır
İşitme ve Konuşma Engelli Bireyler için İşaret Tanıma Sistemi Geliştirme
Authors: Oktekin Bora
Published: April 2019

İşaret dili, el hareketlerinin, parmakların, kolların veya vücut hareketinin oryantasyonu ile konuşanın fikirlerini iletmek için yüz ifadeleriyle eş zamanlı olarak yaptıkları hareketlerdir. İşaret dilleri, son yıllarda tüm araştırmacıların gözdesi konumundadır. Yapılan hareketler sensörler yardımı ile tanınabilmektedir. Ancak, hareket verilerinin bilgisayar sistemlerine aktarılması büyük önem taşımaktadır. Alan yazın incelemesi sonucunda bu yönde yapılan çalışmaların yeterli olmadığı belirlenmiştir. Ayrıca, yapılmış çalışmaların daha çok Amerikan İşaret Dili, İngiliz İşaret Dili ve Arap İşaret Dili yönünde olduğu ve Türk İşaret Dili yönünde yapılan çalışmaların yeterli olmadığı tespit edilmiştir. Bu çalışmada, işitme ve konuşma engelli bireylerin diğer bireyler ile iletişimlerini kolaylaştırabilecek akıllı bir sistem geliştirilmiştir. Bu bağlamda yapılan çalışmanın alan yazındaki bu eksikliğin giderilmesine fayda sağlayacağı düşünülmektedir. Çalışma kapsamında geliştirilen akıllı sistemde, Türk İşaret Dili’nde ses bilimi olarak adlandırılan ve işaretlerin de temelini oluşturan 33 tane temel işaret baz alınmıştır. Bu işaretlerin sistem tarafından tanınabilmesi için Microsoft Kinect v2 sensörü kullanılmıştır. Sistemin altyapısında C# programlama dili ile sınıflandırma algoritmalarından Saklı Markov Modeli ve veritabanı olarak da MongoDB kullanılmıştır. Yapılan vaka çalışması sonucunda; 33 temel işaretin %82’inin geliştirilen sistem tarafından doğru bir şekilde tanımlandığı gözlemlenmiştir. Elde edilen doğruluk oranı göz önünde tutularak geliştirilen işaret tanıma sisteminin hem işitme ve konuşma engelli bireylere, hem de diğer bireylere yardımcı olacağı ve aralarındaki iletişim kurma problemini çözeceği düşünülmektedir
Zihin Engelliler Öğretmenliği Bölümü Öğretmen Adaylarının Özel Eğitim Uygulama M
Authors: Karabıyık Vasfiye
Published: April 2019

Öğretmen adayları özel gereksinimli öğrenciler için öğretimsel düzenlemelerin öğretmenler tarafından nasıl yapıldığını gözlemler, kendi mevcut bilgileri ile eşleştirir ve yapılan uygulamalar hakkında fikir sahibi olur. Özel eğitim kurumlarında öğretmenlerin öğrencilerle yaptığı uygulamalarda farklılıklar olduğu gözlemlenmektedir. Bu öğretmen adaylarının eksik bilgilerle öğretmen yetişmesine yol açabilmektedir. Bu bağlamda öğretmen adaylarının nitelikli bir öğretmen olarak yetiştirilmesi için kurumlarda yapılan öğretimsel düzenlemelere ilişkin görüşlerinin ortaya konulması gerekmektedir. Bu çalışmada öğretmen adaylarının kurumlardaki öğretmenlerin yaptıkları öğretimsel düzenlemelere ilişkin görüşlerinin alınması amaçlanmıştır. Bu çalışma, durum çalışması modelinde nitel araştırmadır. Araştırmada KKTC Milli Eğitim Bakanlığı’na bağlı üç özel eğitim kurumunda aday öğretmen olarak yürütmekte olan amaçlı örneklem yöntemiyle seçilmiş sekiz öğretmen adayı ile görüşme yapılmıştır. Çalışmada veriler niteliksel veri toplama yöntemlerinden yarı yapılandırılmış sorulardan oluşan görüşme formuyla toplanmış ve içerik analizi ile analiz edilmiştir. Araştırma bulguları öğretmen adaylarının; (a) bireyselleştirilmiş eğitim programı, (b) bireyselleştirilmiş öğretim planı, (c) materyal kullanımı, (d) öğretim ortamı, (e) değerlendirme süreci, (f) yetersizlik grupları, (g) disiplinlerarası uygulamalar, (h) öğretim stratejileri, (ı) öğretim sürecinde kullanılan süre ile ilgili konularda yapılan düzenlemelere ilişkin farklı görüş belirttiğini göstermiştir. Araştırma sonuçlarına dayanarak öğretmenlik uygulamalarında öğretmen adayının teorik bilgilerini uygulama ortamına dökebileceği uygulama alanlarının oluşturulması, öğretmenlik uygulaması derslerinin niteliğinin artırılması için kurumdaki öğretmenlerle öğretmen adaylarının işbirliği içinde çalışması önerilebilir
İlköğretim Matematik Öğretmen Adaylarının Akademik Başarılarını Öğrenme Stilleri
Authors: Altun Hasan
Published: April 2019

Bu çalışmada, ilköğretim matematik öğretmen adaylarının tu¨rev konusundaki akademik başarılarının öğrenme stillerine göre incelemesi amaçlanmıştır. Çalışmada genel tarama modeli kullanılmıştır. Çalışmanın örneklemini, 2015-2016 eğitim-öğretim döneminde Buca Eğitim Faku¨ltesi İlköğretim Matematik Öğretmenliği Anabilim Dalı’nda öğrenim gören %75,86’sı (n=154) kadın, %24,14’ü (n=49) erkek olmak üzere toplam 203 öğretmen adayı oluşturmaktadır. Araştırmada veri toplama aracı olarak, öğretmen adaylarının tu¨rev konusundaki akademik başarılarını belirlemek için 25 soruluk çoktan seçmeli başarı testi ve öğrenme stillerini belirlemek için Kolb (1976) tarafından geliştirilen ve Aşkar ve Akkoyunlu (1993) tarafından Türkçe uyarlaması yapılan Kolb Öğrenme Stili Envanteri kullanılmıştır. Araştırmada sonucunda öğrencilerin %39,90’ının özümseyen, %17,24’inin değiştiren, %31,52’ünün ayrıştıran ve %11,34’inin yerleştiren öğrenme stiline sahip olduklarını göstermektedir. İlköğretim matematik öğretmenliği öğrencilerinin tu¨rev konusundaki akademik başarılarının sınıf bağımsız değişkenine göre istatistiksel olarak anlamlı bir farklılaştığı saptanmıştır. İlköğretim matematik öğretmenliği öğrencilerinin sınıf bağımsız değişkenine göre Kolb öğrenme stilleri arasında istatistiksel olarak anlamlı farklılaşmaların olduğu saptanmıştır. İlköğretim matematik öğretmenliği öğrencilerinin Kolb öğrenme stillerinin cinsiyet bağımsız değişkenin etkilenmediği, cinsiyete göre istatistiksel olarak anlamlı bir farklılaşmanın olmadığı bulunmuştur.
Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products
Authors: Djurovic Sanja , Dragicevic Vesna , Waisi Hadi , Pagnacco Maja , Lukovic Nevena , Knezevic-Jugovic Zorica , Nikolic Bogdan
Published: April 2019

Polyphenols present in different plant cell organelles increase the resistance of plants to various types of environmental stresses. We investigated the possibility of increasing the content of bioactive compounds in the seed of yellow soybean variety Laura. The soybean was treated during vegetation with five products based on plant extracts, on the assumption of enrichment of plants with various nutrients. Soybean flour extracts were screened spectrophotometrically for total phenolic content and antioxidant activity. The antioxidant activity was evaluated using three methods. The content of phenolic acids was determined by HPLC, and the raw protein content was estimated by the Kjeldahl method. Depending on the treatment, variations in the quantity of individual phenolic acids with up to 90% higher concentration as compared to the control were observed. Controlled usage of certain plant extracts can increase the concentration of the target group of bioactive compounds in the samples. The synergistic effect of proteins and phenolic compounds on the antioxidant activity of extracts was detected. The results of this study are not only important from the aspect of plant resistance to various types of stress, but also when considering soybean as a functional food. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31018, Grant no. 31037, Grant no. 37021 and Grant no. 172015]
Laser surface texturing with shifted method—Functional surfaces at high speed
Authors: Martan Jiří , Moskal Denys , Kučera Martin
Published: April 2019

Laser surface texturing is a promising technology for future wide applications of functional surfaces with specific properties like hydrophobic, antibacterial, adhesive, self-cleaning, anticorrosion, light absorbing, low friction, etc. Great advancements have been made in this field in the last few years, but in most cases, it takes from minutes up to 1 h to produce 1 cm2 of a functional surface. Even the availability of high-power ultrashort pulsed lasers in the last few years did not dramatically increase productivity, because there are physical limitations of current processing methods: heat accumulation and oxidation, plasma shielding effect, and precision at high speeds. In order to solve these limitations, there have been developed a new method called a shifted laser surface texturing (sLST) method. The new method has a potential to be at least 100 times more productive with no heat accumulation effect and virtually unlimited number of complex shape objects produced with high precision on the surface. In the present work, the principle and advantages of the method are described. The results of the method are compared with two standard methods (path filling of objects and hatch over all objects). The sLST method is presented in both single pulse and burst variants. Examples of its application on different materials for increased adhesion of surface coatings are shown.