bluuebunny/crossref_metadata_2025 huggingface dataset:
Virtual Diagnostic Suite for Electron Beam Prediction and Control at FACET-II
Authors: Emma Claudio , Edelen Auralee , Hanuka Adi , O’Shea Brendan , Scheinker Alexander
Published: January 2021

We discuss the implementation of a suite of virtual diagnostics at the FACET-II facility currently under commissioning at SLAC National Accelerator Laboratory. The diagnostics will be used for the prediction of the longitudinal phase space along the linac, spectral reconstruction of the bunch profile, and non-destructive inference of transverse beam quality (emittance) while using edge radiation at the injector dogleg and bunch compressor locations. These measurements will be folded into adaptive feedbacks and Machine Learning (ML)-based reinforcement learning controls to improve the stability and optimize the performance of the machine for different experimental configurations. In this paper we describe each of these diagnostics with expected measurement results that are based on simulation data and discuss progress towards implementation in regular operations.
Effect of Selection for Pyrethroid Resistance on Abiotic Stress Tolerance in Aedes aegypti from Merida, Yucatan, Mexico
Authors: Amer Keenan , Saavedra-Rodriguez Karla , Black William C. , Gray Emilie M.
Published: January 2021

The study of fitness costs of insecticide resistance mutations in Aedes aegypti has generally been focused on life history parameters such as fecundity, mortality, and energy reserves. In this study we sought to investigate whether trade-offs might also exist between insecticide resistance and other abiotic stress resistance parameters. We evaluated the effects of the selection for permethrin resistance specifically on larval salinity and thermal tolerance. A population of A. aegypti originally from Southern Mexico was split into two strains, one selected for permethrin resistance and the other not. Larvae were reared at different salinities, and the fourth instar larvae were subjected to acute thermal stress; then, survival to both stresses was compared between strains. Contrary to our predictions, we found that insecticide resistance correlated with significantly enhanced larval thermotolerance. We found no clear difference in salinity tolerance between strains. This result suggests that insecticide resistance does not necessarily carry trade-offs in all traits affecting fitness and that successful insecticide resistance management strategies must account for genetic associations between insecticide resistance and abiotic stress resistance, as well as traditional life history parameters.
Renaming NAFLD to MAFLD: Could the LDE System Assist in This Transition?
Authors: Lonardo Amedeo
Published: January 2021

Our understanding of fatty liver syndromes and their relationship with the metabolic syndrome has improved over recent decades and, paralleling this, we are now at the dawn of the NAFLD (nonalcoholic fatty liver disease) to MAFLD (metabolic-associated fatty liver disease) transition. The pitfalls of NAFLD diagnosis, together with disappointing results in therapeutic trials, and the inconsistencies and risks inherent in a “negative” definition (such as “nonalcoholic”) as opposed to a “positive” one (i.e., “metabolic”) are predicted to facilitate the proposed renaming of NAFLD to MAFLD. However, a premature change of terminology would not necessarily address major unmet needs in this area, and may even become counterproductive. As an aid to selecting more homogeneous cohorts of patients, I propose the LDE (Liver, Determinants, Extra-hepatic) classification system which, in principle, may help to assess the natural course of disease as well as the efficacy of novel drugs in patients with NAFLD/MAFLD.
Gastrointestinal Dysfunction in Parkinson’s Disease
Authors: Skjærbæk Casper , Knudsen Karoline , Horsager Jacob , Borghammer Per
Published: January 2021

Parkinson’s disease (PD) is the second most common neurodegenerative disease. Patients show deposits of pathological, aggregated α-synuclein not only in the brain but throughout almost the entire length of the digestive tract. This gives rise to non-motor symptoms particularly within the gastrointestinal tract and patients experience a wide range of frequent and burdensome symptoms such as dysphagia, bloating, and constipation. Recent evidence suggests that progressive accumulation of gastrointestinal pathology is underway several years before a clinical diagnosis of PD. Notably, constipation has been shown to increase the risk of developing PD and in contrast, truncal vagotomy seems to decrease the risk of PD. Animal models have demonstrated gut-to-brain spreading of pathological α-synuclein and it is currently being intensely studied whether PD begins in the gut of some patients. Gastrointestinal symptoms in PD have been investigated by the use of several different questionnaires. However, there is limited correspondence between subjective gastrointestinal symptoms and objective dysfunction along the gastrointestinal tract, and often the magnitude of dysfunction is underestimated by the use of questionnaires. Therefore, objective measures are important tools to clarify the degree of dysfunction in future studies of PD. Here, we summarize the types and prevalence of subjective gastrointestinal symptoms and objective dysfunction in PD. The potential importance of the gastrointestinal tract in the etiopathogenesis of PD is briefly discussed.
Impact of Corticosteroid Administration within 7 Days of the Hospitalization for Influenza Pneumonia with Respiratory Failure: A Propensity Score Analysis Using a Nationwide Administrative Database
Authors: Okuno Daisuke , Kido Takashi , Muramatsu Keiji , Tokutsu Kei , Moriyama Sakiko , Miyamura Takuto , Hara Atsuko , Ishimoto Hiroshi , Yamaguchi Hiroyuki , Miyazaki Taiga , Sakamoto Noriho , Obase Yasushi , Ishimatsu Yuji , Fujino Yoshihisa , Yatera Kazuhiro , Matsuda Shinya , Mukae Hiroshi
Published: January 2021

Influenza pneumonia, which causes acute respiratory distress syndrome and multiple organ failure, has no established management protocol. Recently, corticosteroid therapy was used to treat coronavirus disease 2019 with respiratory failure; however, its effectiveness as a treatment for influenza pneumonia remains controversial. To investigate the impact of corticosteroid therapy for the early phase of severe influenza pneumonia, we compared influenza pneumonia patients with respiratory failure treated with or without corticosteroids within 7 days after hospital admission using a Japanese nationwide administrative database. The primary endpoint was the mortality rate. The secondary endpoints were duration of intensive-care unit management, invasive mechanical ventilation, and hospital stay. The inverse probability weighting method with estimated propensity scores was used to minimize the data collection bias. We included 3519 patients with influenza pneumonia with respiratory failure. Of these, 875 were treated with corticosteroids. There was no significant difference between the groups regarding 30-day and 90-day mortality, duration of intensive-care unit management, invasive mechanical ventilation, and hospital stay. However, the in-hospital mortality rate was higher in the corticosteroid group. The use of systematic corticosteroid therapy in patients with influenza pneumonia was associated with a higher in-hospital mortality rate.
Assessing the Impact of Disruptive Events on Port Performance and Choice: The Case of Gothenburg
Authors: Svanberg Martin , Holm Henrik , Cullinane Kevin
Published: January 2021

This paper assesses the impact of a major disruptive event at the port of Gothenburg, Scandinavia’s largest container port. Automatic Identification System (AIS) data is analyzed, in combination with official port statistics on container handling in the four main container ports in Sweden, from 2014–2018. Particular attention is paid to the relationship between container volumes handled and calculated performance metrics at the specific times of the intense labour dispute at the port of Gothenburg during the periods Q2 (2016) and Q4 (2016)–Q2 (2017). The paper concludes that the decline in container volumes handled at Gothenburg over the period is specifically due to fewer ships calling at the port following each of the intense periods of the labour dispute. It is also concluded that the effect on competitor ports in the region were significant in terms of both increased volumes of gateway container traffic and the resulting short-term and medium term impacts on both port user profiles and port efficiency levels.
Skin Immunomodulation during Regeneration: Emerging New Targets
Authors: Mazini Loubna , Rochette Luc , Hamdan Yousra , Malka Gabriel
Published: January 2021

Adipose-Derived Stem Cells (ADSC) are present within the hypodermis and are also expected to play a pivotal role in wound healing, immunomodulation, and rejuvenation activities. They orchestrate, through their exosome, the mechanisms associated to cell differentiation, proliferation, and cell migration by upregulating genes implicated in different functions including skin barrier, immunomodulation, cell proliferation, and epidermal regeneration. ADSCs directly interact with their microenvironment and specifically the immune cells, including macrophages and T and B cells, resulting in differential inflammatory and anti-inflammatory mechanisms impacting, in return, ADSCs microenvironment and thus skin function. These useful features of ADSCs are involved in tissue repair, where the required cell proliferation, angiogenesis, and anti-inflammatory responses should occur rapidly in damaged sites. Different pathways involved have been reported such as Growth Differentiation Factor-11 (GDF11), Tumor Growth Factor (TGF)-β, Metalloproteinase (MMP), microRNA, and inflammatory cytokines that might serve as specific biomarkers of their immunomodulating capacity. In this review, we try to highlight ADSCs’ network and explore the potential indicators of their immunomodulatory effect in skin regeneration and aging. Assessment of these biomarkers might be useful and should be considered when designing new clinical therapies using ADSCs or their specific exosomes focusing on their immunomodulation activity.
Gut-Brain Axis Cross-Talk and Limbic Disorders as Biological Basis of Secondary TMAU
Authors: Donato Luigi , Alibrandi Simona , Scimone Concetta , Castagnetti Andrea , Rao Giacomo , Sidoti Antonina , D’Angelo Rosalia
Published: January 2021

Background: Trimethylaminuria (TMAU) is a rare metabolic syndrome characterized by the accumulation and the excretion of trimethylamine (TMA), a volatile diet compound produced by gut microbiota. Gut microbiota alterations are mainly involved in the secondary TMAU, whose patients show also different psychiatric conditions. We hypothesized that the biological activity of several molecules acting as intermediate in TMA metabolic reaction might be at the basis of TMAU psychiatric comorbidities. Methods: To corroborate this hypothesis, we performed the analysis of microbiota of both psychiatric suffering secondary TMAU patients and TMAU “mentally ill” controls, comparing the alteration of metabolites produced by their gut bacteria possibly involved in neurotransmission and, in the same time, belonging to biochemical pathways leading to TMA accumulation. Results: Microbiota analyses showed that Clostridiaceae, Lachnospiraceae and Coriobacteriaceae alterations represented the bacterial families with highest variations. This results in an excessive release of serotonin and an hyperactivation of the vagus nerve that might determine the widest spectrum of psychiatric disorders shown by affected patients. These metabolites, as short chain fatty acids, lactate and neurotransmitter precursors, are also related to TMA accumulation. Conclusions: Knowledge of microbiota-gut-brain axis may become a potential new strategy for improving metabolic diseases and to treat linked psychiatric disorders.
Harnessing Extracellular Matrix Biology for Tumor Drug Delivery
Authors: Subrahmanyam Nithya , Ghandehari Hamidreza
Published: January 2021

The extracellular matrix (ECM) plays an active role in cell life through a tightly controlled reciprocal relationship maintained by several fibrous proteins, enzymes, receptors, and other components. It is also highly involved in cancer progression. Because of its role in cancer etiology, the ECM holds opportunities for cancer therapy on several fronts. There are targets in the tumor-associated ECM at the level of signaling molecules, enzyme expression, protein structure, receptor interactions, and others. In particular, the ECM is implicated in invasiveness of tumors through its signaling interactions with cells. By capitalizing on the biology of the tumor microenvironment and the opportunities it presents for intervention, the ECM has been investigated as a therapeutic target, to facilitate drug delivery, and as a prognostic or diagnostic marker for tumor progression and therapeutic intervention. This review summarizes the tumor ECM biology as it relates to drug delivery with emphasis on design parameters targeting the ECM.
Serum Ceramides as Prognostic Biomarkers of Large Thrombus Burden in Patients with STEMI: A Micro-Computed Tomography Study
Authors: Karagiannidis Efstratios , Papazoglou Andreas S. , Stalikas Nikolaos , Deda Olga , Panteris Eleftherios , Begou Olga , Sofidis Georgios , Moysidis Dimitrios V. , Kartas Anastasios , Chatzinikolaou Evangelia , Keklikoglou Kleoniki , Bompoti Andreana , Gika Helen , Theodoridis Georgios , Sianos Georgios
Published: January 2021

ST-elevation myocardial infarction (STEMI) remains one of the leading causes of mortality worldwide. The identification of novel metabolic and imaging biomarkers could unveil key pathophysiological mechanisms at the molecular level and promote personalized care in patients with acute coronary syndromes. We studied 38 patients with STEMI who underwent primary percutaneous coronary intervention and thrombus aspiration. We sought to correlate serum ceramide levels with micro-CT quantified aspirated thrombus volume and relevant angiographic outcomes, including modified TIMI thrombus grade and pre- or post-procedural TIMI flow. Higher ceramide C16:0 levels were significantly but weakly correlated with larger aspirated thrombus volume (Spearman r = 0.326, p = 0.046), larger intracoronary thrombus burden (TB; p = 0.030) and worse pre- and post-procedural TIMI flow (p = 0.049 and p = 0.039, respectively). Ceramides C24:0 and C24:1 were also significantly associated with larger intracoronary TB (p = 0.008 and p = 0.001, respectively). Receiver operating characteristic analysis demonstrated that ceramides C24:0 and C24:1 could significantly predict higher intracoronary TB (area under the curve: 0.788, 95% CI: 0.629–0.946 and 0.846, 95% CI: 0.706–0.985, respectively). In conclusion, serum ceramide levels were higher among patients with larger intracoronary and aspirated TB. This suggests that quantification of serum ceramides might improve risk-stratification of patients with STEMI and facilitate an individualized approach in clinical practice.
Proximity, Familiarity or Congruency? What Influences Memory of Brand Placement in Videogames
Authors: Aliagas Irene , Privado Jesús , Merino M Dolores
Published: January 2021

Brand placement in videogames consists in integrating various brands that are not intrusive to give a feeling of reality to the context, as such increasing awareness and recognition of the brand in the mind of the consumer. The goal of this study is to ascertain if brand position, familiarity and congruency influence memorization of brands presented in a racing videogame. An experimental design 2 (position: prominent or subtle) × 2 (congruency: yes or no) × 2 (familiar: yes or no) was used in a sample of 117 participants (M = 20.91 years, SD = 1.75 years). Consumers have better recall and recognition of brand placement when it is familiar. Likewise, there is more memorization of placement that is congruent with the videogame’s subject matter. Nevertheless, it appears that position has no influence on memory. Regarding the three-way interaction, it turns out that the best way to remember brands is when they are familiar to the consumers, congruent with the videogame’s genre, and they are positioned in a prominent position. This article is the first to investigate the interaction between the three main variables that affect the memorization of brand placement.
The Impact of Borrowing Size on the Economic Development of Small and Medium-Sized Cities in China
Authors: Gong Xiaoxia , Zhong Fanglei
Published: January 2021

Sharing the economic agglomeration effects of large cities is considered an effective way to enhance economic growth in small and medium-sized cities, yet there remains a lack of relevant quantitative empirical research. In this study, the three dimensions of borrowing size of 285 prefecture-level cities and nighttime lighting data from 2004 to 2013 in China are used to assess its effect on economic development by a fixed-effects model with panel data. The results show that first, the effect of borrowing size on small and medium-sized cities’ economic development is significantly positive. In contrast, the effect of borrowing size on large cities is not significant. Second, the magnitude of the impact of borrowing size on small and medium-sized cities varies considerably across regions. In middle and western China, the most significant positive effect is from borrowing economic activity density and borrowing advanced functions. However, in eastern China, the most significant positive effect is from borrowing population, while borrowing advanced functions has a significant negative effect. In the northeast, borrowing economic activity density has a significant positive effect. This article provides policy recommendations in three areas: improving intercity accessibility, rationalizing the layout of urban industries, and supporting the development of education.
Rethinking the Relationship between Women, Crime and Economic Factors: The Case-Study of Women Sentenced to Death for Drug Trafficking in Malaysia
Authors: Harry Lucy
Published: January 2021

This paper draws upon my doctoral research into the experiences of women who have been sentenced to death for drug trafficking in Malaysia. I utilise this case-study as a lens through which to examine the relationship between women, crime and economic factors. From my data derived from 47 ‘elite’ interviews, as well as legal and media database searches (resulting in information on 146 cases), I argue that current feminist criminological theorising should be updated to incorporate the relationship between women’s crime and precarious work. As I show, precarity is gendered and disproportionately affects women from the global south. Overall, I find that many of the women who have been sentenced to death in Malaysia were engaged in precarious work and drug trafficking was a way to make ‘quick money’ to address economic insecurity. Clearly, capital punishment is incommensurate with the crime.
Dectin-1-Mediated DC-SIGN Recruitment to Candida albicans Contact Sites
Authors: Choraghe Rohan P. , Neumann Aaron K.
Published: January 2021

At host–pathogen contact sites with Candida albicans, Dectin-1 activates pro-inflammatory signaling, while DC-SIGN promotes adhesion to the fungal surface. We observed that Dectin-1 and DC-SIGN collaborate to enhance capture/retention of C. albicans under fluid shear culture conditions. Therefore, we devised a cellular model system wherein we could investigate the interaction between these two receptors during the earliest stages of host–pathogen interaction. In cells expressing both receptors, DC-SIGN was quickly recruited to contact sites (103.15% increase) but Dectin-1 did not similarly accumulate. Once inside the contact site, FRAP studies revealed a strong reduction in lateral mobility of DC-SIGN (but not Dectin-1), consistent with DC-SIGN engaging in multivalent adhesive binding interactions with cell wall mannoprotein ligands. Interestingly, in the absence of Dectin-1 co-expression, DC-SIGN recruitment to the contact was much poorer—only 35.04%. These data suggested that Dectin-1 promotes the active recruitment of DC-SIGN to the contact site. We proposed that Dectin-1 signaling activates the RHOA pathway, leading to actomyosin contractility that promotes DC-SIGN recruitment, perhaps via the formation of a centripetal actomyosin flow (AMF) directed into the contact site. Indeed, RHOA pathway inhibitors significantly reduced Dectin-1-associated DC-SIGN recruitment to the contact site. We used agent-based modeling to predict DC-SIGN transport kinetics with (“Directed + Brownian”) and without (“Brownian”) the hypothesized actomyosin flow-mediated transport. The Directed + Brownian transport model predicted a DC-SIGN contact site recruitment (106.64%), similar to that we observed experimentally under receptor co-expression. Brownian diffusive transport alone predicted contact site DC-SIGN recruitment of only 55.60%. However, this value was similar to experimentally observed DC-SIGN recruitment in cells without Dectin-1 or expressing Dectin-1 but treated with RHOA inhibitor, suggesting that it accurately predicted DC-SIGN recruitment when a contact site AMF would not be generated. TIRF microscopy of nascent cell contacts on glucan-coated glass revealed Dectin-1-dependent DC-SIGN and F-actin (LifeAct) recruitment kinetics to early stage contact site membranes. DC-SIGN entry followed F-actin with a temporal lag of 8.35 ± 4.57 s, but this correlation was disrupted by treatment with RHOA inhibitor. Thus, computational and experimental evidence provides support for the existence of a Dectin-1/RHOA-dependent AMF that produces a force to drive DC-SIGN recruitment to pathogen contact sites, resulting in improved pathogen capture and retention by immunocytes. These data suggest that the rapid collaborative response of Dectin-1 and DC-SIGN in early contact sties might be important for the efficient acquisition of yeast under flow conditions, such as those that prevail in circulation or mucocutaneous sites of infection.
Reproduction and the Early Development of Vertebrates in Space: Problems, Results, Opportunities
Authors: Proshchina Alexandra , Gulimova Victoria , Kharlamova Anastasia , Krivova Yuliya , Besova Nadezhda , Berdiev Rustam , Saveliev Sergey
Published: January 2021

Humans and animals adapt to space flight conditions. However, the adaptive changes of fully formed organisms differ radically from the responses of vertebrate embryos, foetuses, and larvae to space flight. Development is associated with active cell proliferation and the formation of organs and systems. The instability of these processes is well known. Over 20 years has passed since the last systematic experiments on vertebrate reproduction and development in space flight. At the same time, programs are being prepared for the exploration of Mars and the Moon, which justifies further investigations into space flight’s impact on vertebrate development. This review focuses on various aspects of reproduction and early development of vertebrates in space flights. The results of various experiments on fishes, amphibians, reptiles, birds and mammals are described. The experiments in which our team took part and ontogeny of the vertebrate nervous and special sensory systems are considered in more detail. Possible causes of morphological changes are also discussed. Research on evolutionarily and taxonomically different models can advance the understanding of reproduction in microgravity. Reptiles, in particular, geckos, due to their special features, can be a promising object of space developmental biology.
Mixed Ionic-Electronic Conductivity, Redox Behavior and Thermochemical Expansion of Mn-Substituted 5YSZ as an Interlayer Material for Reversible Solid Oxide Cells
Authors: Natoli Alejandro , Arias-Serrano Blanca I. , Rodríguez-Castellón Enrique , Żurawska Agnieszka , Frade Jorge R. , Yaremchenko Aleksey. A.
Published: January 2021

Manganese-substituted 5 mol.% yttria-stabilized zirconia (5YSZ) was explored as a prospective material for protective interlayers between electrolyte and oxygen electrodes in reversible solid oxide fuel/electrolysis cells. [(ZrO2)0.95(Y2O3)0.05]1−x[MnOy]x (x = 0.05, 0.10 and 0.15) ceramics with cubic fluorite structure were sintered in air at 1600 °C. The characterization included X-ray diffraction (XRD), scanning electron microscopy (SEM)/energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), thermogravimetry and dilatometry in controlled atmospheres, electrical conductivity measurements, and determination of oxygen-ion transference numbers by the electromotive force (EMF) technique. Mn-substituted 5YSZ solid solutions exhibit variable oxygen nonstoichiometry with manganese cations in a mixed 2+/3+ oxidation state under oxidizing conditions. Substitution by manganese gradually increases the extent of oxygen content variation on thermal/redox cycling, chemical contribution to thermal expansion and dimensional changes on reduction. It also deteriorates oxygen-ionic conductivity and improves p-type electronic conductivity under oxidizing conditions, leading to a gradual transformation from predominantly ionic to prevailing electronic transport with increasing x. Mn2+/3+→Mn2+ transformation under reducing atmospheres is accompanied by the suppression of electronic transport and an increase in ionic conductivity. All Mn-substituted 5YSZ ceramics are solid electrolytes under reducing conditions. Prolonged treatments in reducing atmospheres, however, promote microstructural changes at the surface of bulk ceramics and Mn exsolution. Mn-substituted 5YSZ with 0.05 ≤ x < 0.10 is considered the most suitable for the interlayer application, due to the best combination of relevant factors, including oxygen content variations, levels of ionic/electronic conductivity and thermochemical expansion.
Influence of Lime and Volcanic Ash on the Properties of Dune Sand as Sustainable Construction Materials
Authors: Shalabi Faisal I. , Mazher Javed , Khan Kaffayatullah , Amin Muhammad Nasir , Albaqshi Alaa , Alamer Abdullah , Barsheed Ali , Alshuaibi Othman
Published: January 2021

This study focused on evaluating dune sand stabilized with lime and volcanic ash as base course materials in engineering construction. Dune sands are found in Saudi Arabia in huge quantities. Due to the high demand for construction materials, this makes them highly suitable for construction. A testing program was designed to investigate the effect of adding different percentages by weight of lime (L: 0, 2, 4, and 6%) and volcanic ash (VA: 0, 1, 3, and 5%) on the engineering properties of the stabilized mixture. Unconfined compressive strength (UCS) and California bearing ratio (CBR) tests were conducted. In addition, Raman spectroscopy and laser-scanning microscopy (LSM) tests were performed to explore the chemical characteristic, packing, and structure of the mixture. The results showed that the UCS, CBR, and the Young’s modulus (Es) of the treated dune sand increased with the increase in percentage of both stabilizers. Furthermore, LSM images of mortar blended with intermediate L-to-VA blend ratio ≈0.55 (L: 6% and VA: 5%) exhibit compact packing of sand grains, indicating strong adhesion and higher cementing value. The results of the study are promising and encourage using the treated dune sand in engineering construction even with a low percentage use of lime (2%) and volcanic ash (1–3%) as stabilizers.
Spatiotemporal Analysis of Hydration Mechanism in Sodium Alginate Matrix Tablets
Authors: Juszczyk Ewelina , Kulinowski Piotr , Baran Ewelina , Birczyński Artur , Majda Dorota , García-Montoya Encarna , Pérez-Lozano Pilar , Suñé-Negre Josep Maria , Węglarz Władysław P. , Dorożyński Przemysław
Published: January 2021

Methods of spatiotemporal characterization of nonequilibrated polymer based matrices are still immature and imperfect. The purpose of the study was to develop the methodology for the spatiotemporal characterization of water transport and properties in alginate tablets under hydration. The regions of low water content were spatially and temporally sampled using Karl Fisher and Differential Scanning Callorimetry (spatial distribution of freezing/nonfreezing water) with spatial resolution of 1 mm. In the regions of high water content, where sampling was infeasible due to gel/sol consistency, magnetic resonance imaging (MRI) enabled characterization with an order of magnitude higher spatial resolution. The minimally hydrated layer (MHL), infiltration layer (IL) and fully hydrated layer (FHL) were identified in the unilaterally hydrated matrices. The MHL gained water from the first hour of incubation (5–10% w/w) and at 4 h total water content was 29–39% with nonfreezing pool of 28–29%. The water content in the IL was 45–47% and at 4 h it reached ~50% with the nonfreezing pool of 28% and T2 relaxation time < 10 ms. The FHL consisted of gel and sol layer with water content of 85–86% with a nonfreezing pool of 11% at 4 h and T2 in the range 20–200 ms. Hybrid destructive/nondestructive analysis of alginate matrices under hydration was proposed. It allowed assessing the temporal changes of water distribution, its mobility and interaction with matrices in identified layers.
Numerical Analysis of Axial Cyclic Behavior of FRP Retrofitted CHS Joints
Authors: Alembagheri Mohammad , Rashidi Maria , Yazdi Amin , Samali Bijan
Published: January 2021

This paper aims to numerically investigate the cyclic behavior of retrofitted and non-retrofitted circular hollow section (CHS) T-joints under axial loading. Different joints with varying ratios of brace to chord radius are studied. The effects of welding process on buckling instability of the joints in compression and the plastic failure in tension are considered. The finite element method is employed for numerical analysis, and the SAC protocol is considered as cyclic loading scheme. The CHS joints are retrofitted with different numbers of Fiber Reinforced Polymer (FRP) layers with varying orientation. The results show that the welding process significantly increases the plastic failure potential. The chord ovalization is the dominant common buckling mode under the compression load. However, it is possible to increase the energy dissipation of the joints by utilizing FRP composite through changing the buckling mode to the brace overall buckling.
Effect of Restorative Material on Mechanical Response of Provisional Endocrowns: A 3D—FEA Study
Authors: Tribst João Paulo Mendes , Borges Alexandre Luiz Souto , Silva-Concílio Laís Regiane , Bottino Marco Antonio , Özcan Mutlu
Published: January 2021

The goal of this study was to evaluate the stress distribution in an endocrown restoration according to different provisional restorative materials. An endodontically treated maxillary molar model was selected for conducting the finite element analysis (FEA), with a determined amount of dental remnant of 1.5 mm. The model was imported to the analysis software (ANSYS 19.2, ANSYS Inc., Houston, TX, USA) in STEP format. All contacts were considered perfectly bonded. The mechanical properties of each structure were considered isotropic, linear, elastic, and homogeneous. Three different provisional restorative materials were simulated (acrylic resin, bis-acrylic resin, and resin composite). An axial load (300 N) was applied at the occlusal surface in the center of the restoration. Results were determined by colorimetric stress maps of maximum principal stress, maximum shear stress, and total deformation. The different materials influenced the stress distribution for all structures; the higher the material’s elastic modulus, the lower the stress magnitude on the cement layer. In the present study, all provisional restorative materials showed similar stress patterns in the endocrown and on the cement layer however, with different magnitude. Based on this study limitation, the use of resin composite to manufacture provisional endocrowns is suggested as a promising material to reduce the stresses in the cement layer and in the dental tissue surfaces.
Storable bFGF-Releasing Membrane Allowing Continuous Human iPSC Culture
Authors: Oyane Ayako , Araki Hiroko , Nakamura Maki , Aiki Yasuhiko , Ito Yuzuru
Published: January 2021

Basic fibroblast growth factor (bFGF) is a crucial supplement for culture media of human pluripotent stem cells. However, bFGF is extremely unstable under cell culture conditions, which makes frequent (generally every day) medium refreshment requisite. We recently developed a water-floatable, bFGF-releasing membrane via a simple bFGF adsorption process following oxygen plasma treatment by utilizing a polyethylene nonwoven fabric as an adsorbent. This membrane allowed sustained release of bFGF while floating on medium, thereby keeping the bFGF concentration in the medium sufficient for maintaining human-induced pluripotent stem cells (iPSCs) in a proliferative and pluripotent state for as long as 3 days. In this study, lyophilization was applied to the membrane to stabilize bFGF. The sustained bFGF-releasing function of the membrane was kept unchanged even after lyophilization and subsequent cryopreservation at −30 °C for 3 months. The cryopreserved membrane supported proliferation and colony formation of human iPSCs while retaining their viability and pluripotency in a medium-change-free continuous culture for 3 days. The present bFGF-releasing membrane is ready-to-use, storable for at least 3 months, and obviates daily medium refreshment. Therefore, it is a new and more practical bFGF supplement for culture media of human stem cells.
Ordering Awad–Varma Entropy and Applications to Some Stochastic Models
Authors: Sfetcu Răzvan-Cornel , Sfetcu Sorina-Cezarina , Preda Vasile
Published: January 2021

We consider a generalization of Awad–Shannon entropy, namely Awad–Varma entropy, introduce a stochastic order on Awad–Varma residual entropy and study some properties of this order, like closure, reversed closure and preservation in some stochastic models (the proportional hazard rate model, the proportional reversed hazard rate model, the proportional odds model and the record values model).
Two-Variable Type 2 Poly-Fubini Polynomials
Authors: Muhiuddin Ghulam , Khan Waseem Ahmad , Duran Ugur
Published: January 2021

In the present work, a new extension of the two-variable Fubini polynomials is introduced by means of the polyexponential function, which is called the two-variable type 2 poly-Fubini polynomials. Then, some useful relations including the Stirling numbers of the second and the first kinds, the usual Fubini polynomials, and the higher-order Bernoulli polynomials are derived. Also, some summation formulas and an integral representation for type 2 poly-Fubini polynomials are investigated. Moreover, two-variable unipoly-Fubini polynomials are introduced utilizing the unipoly function, and diverse properties involving integral and derivative properties are attained. Furthermore, some relationships covering the two-variable unipoly-Fubini polynomials, the Stirling numbers of the second and the first kinds, and the Daehee polynomials are acquired.
Small-Scale Preparation of Fluorescently Labeled Chemical Probes from Marine Cyclic Peptides, Kapakahines A and F
Authors: Kamihira Rie , Nakao Yoichi
Published: January 2021

A number of bioactive marine natural products have been isolated so far, but it is still difficult to disclose their modes of action. In this study, we prepared fluorescently labeled chemical probes from the cytotoxic marine cyclic peptides kapakahines A (1) and F (2) to visualize their localization as the first step of the study of their modes of action. We used fluorescent dyes 3a or 3a/b (a 1:1 mixture of 3a and 3b) whose terminal N-hydroxysuccinimide (NHS) group can react with the free amino groups of kapakahines. The fluorescently labeled kapakahine A (Kap A-5-FL, 5a) stained P388 murine leukemia cells and HeLa human cervical cancer cells, while cells treated with fluorescently labeled kapakahine F (Kap F-5-FL, 6a) only weakly stained them. Further analysis of the confocal images of the stained cells with higher magnification (×100) indicated the localization of Kap A-5-FL (5a) in the cells. In this paper, we report the small-scale preparation and a new delivery method of fluorescent probes, as well as the application of these procedures to cell staining.
Briarenols W–Z: Chlorine-Containing Polyoxygenated Briaranes from Octocoral Briareum stechei (Kükenthal, 1908)
Authors: Chen You-Ying , Zhang Yi-Lin , Lee Gene-Hsiang , Tsou Lun Kelvin , Zhang Mingzi M. , Hsieh Hsing-Pang , Chen Jih-Jung , Ko Chou-Yuan , Wen Zhi-Hong , Sung Ping-Jyun
Published: January 2021

Briareum stechei is proven to be a rich source of 3,8-cyclized cembranoids (briarane) with a bicyclo[8.4.0] carbon core. In the present study, four previously unreported briaranes, briarenols W–Z (1–4), along with solenolide A (5), briarenolide M (6), briaexcavatolide F (7), and brianolide (8), were isolated and characterized through spectroscopic analysis, and the absolute configuration of 8 was corroborated by a single-crystal x-ray diffraction analysis. Briaranes 2 and 5 were found to induce significant inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophage cells by enhancing the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins.
Estradiol Removal by Adsorptive Coating of a Microfiltration Membrane
Authors: Niavarani Zahra , Breite Daniel , Prager Andrea , Abel Bernd , Schulze Agnes
Published: January 2021

This work demonstrates the enhancement of the adsorption properties of polyethersulfone (PES) microfiltration membranes for 17β-estradiol (E2) from water. This compound represents a highly potent endocrine-disrupting chemical (EDC). The PES membranes were modified with a hydrophilic coating functionalized by amide groups. The modification was performed by the interfacial reaction between hexamethylenediamine (HMD) or piperazine (PIP) as the amine monomer and trimesoyl chloride (TMC) or adipoyl chloride (ADC) as the acid monomer on the surface of the membrane using electron beam irradiation. The modified membranes and the untreated PES membrane were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), water permeance measurements, water contact angle measurements, and adsorption experiments. Furthermore, the effects of simultaneous changes in four modification parameters: amine monomer types (HMD or PIP), acid monomer types (TMC or ADC), irradiation dosage (150 or 200 kGy), and the addition of toluene as a swelling agent, on the E2 adsorption capacity were investigated. The results showed that the adsorption capacities of modified PES membranes toward E2 are >60%, while the unmodified PES membrane had an adsorption capacity up to 30% for E2 under similar experimental conditions, i.e., an enhancement of a factor of 2. Next to the superior adsorption properties, the modified PES membranes maintain high water permeability and no pore blockage was observed. The highlighted results pave the way to develop efficient low-cost, stable, and high-performance adsorber membranes.
Experimental Investigation and Multi-Response Optimization of Machinability of AA5005H34 Using Composite Desirability Coupled with PCA
Authors: Qazi Mohsin Iqbal , Abas Muhammad , Khan Razaullah , Saleem Waqas , Pruncu Catalin Iulian , Omair Muhammad
Published: January 2021

Minimum quantity lubricant (MQL) is an advanced technique in machining to achieve sustainability, productivity, higher precision, economic benefits, and a reduction in carbon footprints. The present research work aims to investigate the effect of the cutting process parameters of the end milling of AA5005H34 material under dry and MQL cutting environments. The key performance indicators of machining include the surface roughness profile, the material removal rate, and tool wear. Surface roughness parameters are measured with the help of the Mitutoyo surface roughness tester, and the cutting tool wear is measured according to the ISO 8688-2:1989 standard using a scanning electron microscope (SEM). Sixteen experiments are designed based on the Taguchi orthogonal array mixture design. Single responses are optimized based on signal to noise ratios, while for multi-response optimization composite desirability function coupled with principal component analysis is applied. Analysis of variance (ANOVA) results revealed that the feed rate followed by spindle speed, axial depth of the cut, width of the cut, and cutting environment are the most significant factors contributing to the surface roughness profile, material removal rate, and tool wear. The optimized parameters are obtained as cutting speed of 3000 rev/min, feed rate of 350 mm/min, axial depth of cut of 2 mm, and width of cut of 6 mm under an MQL environment.
Sensing and Signaling of Methionine Metabolism
Authors: Lauinger Linda , Kaiser Peter
Published: January 2021

Availability of the amino acid methionine shows remarkable effects on the physiology of individual cells and whole organisms. For example, most cancer cells, but not normal cells, are hyper dependent on high flux through metabolic pathways connected to methionine, and diets restricted for methionine increase healthy lifespan in model organisms. Methionine’s impact on physiology goes beyond its role in initiation of translation and incorporation in proteins. Many of its metabolites have a major influence on cellular functions including epigenetic regulation, maintenance of redox balance, polyamine synthesis, and phospholipid homeostasis. As a central component of such essential pathways, cells require mechanisms to sense methionine availability. When methionine levels are low, cellular response programs induce transcriptional and signaling states to remodel metabolic programs and maintain methionine metabolism. In addition, an evolutionary conserved cell cycle arrest is induced to ensure cellular and genomic integrity during methionine starvation conditions. Methionine and its metabolites are critical for cell growth, proliferation, and development in all organisms. However, mechanisms of methionine perception are diverse. Here we review current knowledge about mechanisms of methionine sensing in yeast and mammalian cells, and will discuss the impact of methionine imbalance on cancer and aging.
Transcriptomic Analysis Reveals the Sexually Divergent Host–Wolbachia Interaction Patterns in a Fig Wasp
Authors: Hou Hong-Xia , Zhao Dan , Xiao Jin-Hua , Huang Da-Wei
Published: January 2021

Wolbachia are widely distributed in arthropods and nematodes, acquiring nutrients from the hosts, and inducing remarkable reproductive modulations on the hosts. To investigate the interaction of Wolbachia and insects, Wolbachia are often artificially eliminated from Wolbachia-infected hosts, which may produce negative effects of antibiotics. In the present study, based on the transcriptomic data of a fig wasp species Ceratosolen solmsi with two sibling lineages, one natively infected and the other noninfected with Wolbachia, we investigated the expression patterns of genes. The comparison results of differently expressed genes (DEGs) between Wolbachia infected and noninfected samples show that males have many more DEGs than females. The male unique upregulated genes are enriched in biological processes mainly related to biosynthesis, transport, positive regulation of I-kappaB kinase/NF-kappaB signaling, MAPK cascade, and pathogenesis; the male unique downregulated genes are enriched in biological processes mainly related to transport, oxidation–reduction, cellular responses to oxidative stress, lipid oxidation, cytoskeleton organization, actin filament-based process, and localization. In addition, for the Wolbachia’s gene expression, the number of genes up-regulated in males is higher than that in females. The results revealed divergent patterns of the host–Wolbachia interactions between males and females in the fig wasp species.
Variation among Metschnikowia pulcherrima Isolates for Genetic Modification and Homologous Recombination
Authors: Moreno-Beltrán Mauro , Gore-Lloyd Deborah , Chuck Christopher , Henk Daniel
Published: January 2021

Metschnikowia pulcherrima is a non-conventional yeast with the potential to be used in biotechnological processes, especially involving low-cost feedstock exploitation. However, there are a lack of tools for researching it at a molecular level and for producing genetically modified strains. We tested the amenability to genetic modification of ten different strains, establishing a transformation protocol based on LiAc/PEG that allows us to introduce heterologous DNA. Non-homologous integration was broadly successful and homologous recombination was successful in two strains. Chemical inhibition of non-homologous end joining recombination had a modest effect on the improvement of homologous recombination rates. Removal of selective markers via flippase recombinase was successful across integrated loci except for those targeted to the native URA3 locus, suggesting that the genome sequence or structure alters the efficacy of this system.
Au‐Ag‐S‐Se‐Cl‐Br Mineralization at the Corrida Deposit (Russia) and Physicochemical Conditions of Ore Formation
Authors: Kolova Elena E. , Savva Nataly E. , Zhuravkova Tatiana V. , Glukhov Anton N. , Palyanova Galina A.
Published: January 2021

The mineral and chemical compositions of ores from the Corrida epithermal Au-Ag deposit (Chukchi Peninsula, Russia) were studied using the optical and scanning electron microscopy with X-ray energy-dispersion microanalysis. The deposit was formed at the time close to the period when the basic volume of acid magmas had been emplaced within the Okhotsk–Chukotka belt (84 to 80 Ma). The Au–Ag mineralization is distinguished with Au-Ag sulphides and selenides (uytenbogaardtite-fischesserite solid solution, Se-acanthite, S-naumannite) and Ag halides of the chlorargyrite-embolite-bromargyrite series. The ores were formed in two stages. Using microthermometric methods, it has been established that the ore-bearing quartz was formed in the medium-temperature environment (340–160 °C) with the participation of low-salt (3.55 to 0.18 wt.% NaCl eq.) hydrotherms, mostly of the NaCl composition with magnesium, iron and low-density СО2. According to our results of thermodynamic modeling at temperatures from 300 to 25 °C and data on mineral metasomatic alterations of the host rocks, the Au-Ag-S-Se-Cl-Br mineralization was formed at decreasing temperature and fugacity of sulphur (logƒS2 from −6 to −27), selenium (logƒSe2 from −14 to −35), and oxygen (logƒО2 from −36 to −62), with near-neutral solutions replaced by acid solutions. Analysis of the obtained data shows that the Corrida refers to the group of the LS-type epithermal deposits. This deposit is a new example of epithermal deposits with significant quantities of Au–Ag chalcogenides (acanthite, uytenbogaardtite, fischesserite, naumannite and others).