bluuebunny/crossref_metadata_2025 huggingface dataset:
A Novel Regulator PepR Regulates the Expression of Dipeptidase Gene pepV in Bacillus thuringiensis
Authors: Zhang Xin , Wang Hengjie , Yan Tinglu , Chen Yuhan , Peng Qi , Song Fuping
Published: March 2024

Bacillus thuringiensis produces insecticidal crystal proteins encoded by cry or cyt genes and targets a variety of insect pests. We previously found that a strong promoter of a DeoR family transcriptional regulator (HD73_5014) can efficiently drive cry1Ac expression in B. thuringiensis HD73. Here, we investigated the regulation of neighbor genes by HD73_5014. The HD73_5014 homologs are widely distributed in Gram-positive bacterial species. Its neighbor genes include pepV, rsuA, and ytgP, which encode dipeptidase, rRNA pseudouridine synthase and polysaccharide biosynthesis protein, respectively. The four open reading frames (ORFs) are organized to be a pepR gene cluster in HD73. RT-PCR analysis revealed that the rsuA and ytgP genes formed a transcriptional unit (rsuA-ytgP operon), while pepV formed a transcriptional unit in HD73. Promoter-lacZ fusion assays showed that the pepV and rsuA-ytgP promoters are regulated by HD73_5014. EMSA experiments showed that HD73_5014 directly binds to the pepV promoter region but not to the rusA-ytgP promoter region. Thus, the HD73_5014 transcriptional regulator, which controls the expression of the dipeptidase pepV, was named PepR (dipeptidase regulator). We also confirmed the direct regulation between PepR and PepV by the increased sensitivity to vancomycin in ΔpepV and ΔpepR mutants compared to HD73.
Acanthamoeba spp. as Factors for Severe Infectious Diseases in Humans
Authors: Chomicz Lidia , Szaflik Jacek P. , Baltaza Wanda
Published: March 2024

Acanthamoeba spp [...]
The Causality between Human Immunoglobulin G (IgG) N-Glycosylation and Aging: A Mendelian Randomization Study
Authors: Sun Wenxin , Jian Xuening , Zhang Jie , Meng Xiaoni , Wang Haotian , Zheng Deqiang , Wu Lijuan , Wang Youxin
Published: March 2024

Background: Immunoglobulin G (IgG) N-glycosylation is considered a potential biomarker for aging and various pathological conditions. However, whether these changes in IgG N-glycosylation are a consequence or a contributor to the aging process remains unclear. This study aims to investigate the causality between IgG N-glycosylation and aging using Mendelian randomization (MR) analysis. Methods: We utilized genetic variants associated with IgG N-glycosylation traits, the frailty index (FI), and leukocyte telomere length (LTL) from a previous genome-wide association study (GWAS) on individuals of European ancestry. Two-sample and multivariable MR analyses were conducted, employing the inverse-variance weighted (IVW) method. Sensitivity analyses were performed to assess potential confounding factors. Results: Using the IVW method, we found suggestive evidence of a causal association between GP14 and FI (β 0.026, 95% CI 0.003 to 0.050, p = 0.027) and LTL (β −0.020, 95% CI −0.037 to −0.002, p = 0.029) in the two-sample MR analysis. In the multivariable MR analysis, suggestive evidence was found for GP23 and FI (β −0.119, 95% CI −0.219 to −0.019, p = 0.019) and GP2 and LTL (β 0.140, 95% CI 0.020 to 0.260, p = 0.023). Conclusions: In conclusion, our results supported a potentially causal effect of lower GP23 levels on an advanced aging state. Additional verification is required to further substantiate the causal relationship between glycosylation and aging.
Physical Grinding of Prefabricated Co3O4 and MCM-22 Zeolite for Fischer–Tropsch Synthesis: Impact of Pretreatment Procedure on the Dispersion and Catalytic Performance
Authors: Ren Hua-Ping , Xie Zhi-Xia , Tian Shao-Peng , Ding Si-Yi , Ma Qiang , Zhao Yu-Zhen , Zhang Zhe , Fu Jiao-Jiao , Hao Qing-Qing
Published: March 2024

To improve the mess-specific activity of Co supported on zeolite catalysts in Fischer–Tropsch (FT) synthesis, the Co-MCM-22 catalyst was prepared by simply grinding the MCM-22 with nanosized Co3O4 prefabricated by the thermal decomposition of the Co(II)-glycine complex. It is found that this novel strategy is effective for improving the mess-specific activity of Co catalysts in FT synthesis compared to the impregnation method. Moreover, the ion exchange and calcination sequence of MCM-22 has a significant influence on the dispersion, particle size distribution, and reduction degree of Co. The Co-MCM-22 prepared by the physical grinding of prefabricated Co3O4 and H+-type MCM-22 without a further calcination process exhibits a moderate interaction between Co3O4 and MCM-22, which results in the higher reduction degree, higher dispersion, and higher mess-specific activity of Co. Thus, the newly developed method is more controllable and promising for the synthesis of metal-supported catalysts.
Could the Length of the Alkyl Chain Affect the Photodynamic Activity of 5,10,15,20-Tetrakis(1-alkylpyridinium-4-yl)porphyrins?
Authors: Malacarne Miryam Chiara , Gariboldi Marzia Bruna , Marras Emanuela , Caruso Enrico
Published: March 2024

Photodynamic therapy (PDT) is a minimally invasive treatment that uses the combination of a photosensitizing agent (PS) and light to selectively target solid tumors, as well as several non-neoplastic proliferating cell diseases. After systemic administration, PSs are activated by localized irradiation with visible light; in the presence of adequate concentrations of molecular oxygen, this causes the formation of reactive oxygen species (ROS) and subsequent tissue damage. In this study, two series of tetrakis(N-alkylpyridinium-4-yl)porphyrins were synthesized, differing in the presence or absence of a zinc ion in the tetrapyrrole nucleus, as well as in the N-alkyl chain length (from one to twelve carbon atoms). The compounds were chemically characterized, and their effect on cell viability was evaluated using a panel of three tumor cell lines to determine a possible relationship between photodynamic activity and Zn presence/alkyl chain length. The types of cell death mechanisms involved in the effect of the various PSs were also evaluated. The obtained results indicate that the most effective porphyrin is the Zn-porphyrin, with a pendant made up of eight carbon atoms (Zn-C8).
Structure, Synthesis, and Catalytic Performance of Emerging MXene-Based Catalysts
Authors: Sun Zhengxiang , Wang Rui , Matulis Vitaly Edwardovich , Vladimir Korchak
Published: March 2024

As traditional fossil fuel energy development faces significant challenges, two-dimensional layered materials have become increasingly popular in various fields and have generated widespread research interest. MXene is an exceptional catalytic material that is typically integrated into functional composite materials with other substances to enhance its catalytic-reaction performance. Improving the thermal stability, electrical conductivity, and electrochemical activity, as well as enhancing the specific surface structure, can make the material an excellent catalyst for photoelectrocatalysis and energy-regeneration reactions. The article mainly outlines the structural characteristics, preparation methods, and applications of MXene in the field of catalysis. This text highlights the latest progress and performance comparison of MXene-based catalytic functional materials in various fields such as electrochemical conversion, photocatalysis, renewable energy, energy storage, and carbon capture and conversion. It also proposes future prospects and discusses the current bottlenecks and challenges in the development of MXene-based catalytic materials.
The Use of Chitin for the Removal of Nitrates and Orthophosphates from Greenhouse Wastewater
Authors: Jóźwiak Tomasz , Mielcarek Artur , Filipkowska Urszula
Published: March 2024

The study investigated the possibility of using chitin flakes as an unconventional sorbent for the removal of orthophosphates and nitrates from greenhouse wastewater (GW). The effluent parameters were as follows: 66.2 mg P-PO4/L, 566.0 mg N-NO3/L, 456.0 mg S-SO4/L, 13.7 mg Cl−/L, 721 mg Ca2+/L, 230 mg Mg2+/L, hardness 11.3 °dH, and pH 5.4. The scope of the research included determinations of the influence of pH on GW composition and the efficiency of nutrient sorption, the kinetics of nutrient sorption, the influence of the dose of chitin flakes on the effectiveness of nutrient binding and the maximum sorption capacity of the sorbent. The sorption of P-PO4 on the tested sorbent was most effective at pH 4, and the sorption of N-NO3 at pH 2. The equilibrium time of sorption of both nutrients from GW to chitin depended on the sorbent dose and ranged from 150 to 180 min. The sorbent dose of 40 g/L enabled removing 90% of orthophosphates and 5.7% of nitrates from the wastewater. The maximum sorption capacity of CH towards P-PO4 and N-NO3 contained in the GW was 3.20 mg/g and 3.04 mg/g, respectively. In turn, the sorption of calcium and magnesium ions on chitin flakes was completely ineffective.
Enhanced Stability of Oral Vitamin C Delivery: A Novel Large-Scale Method for Liposomes Production and Encapsulation through Dynamic High-Pressure Microfluidization
Authors: Romano Eugenia , Palladino Roberta , Cannavale Mariagabriella , Lamparelli Erwin Pavel , Maglione Barbara
Published: March 2024

In recent years, nanocarriers have been widely used as an effective solution for oral administration of pharmaceuticals. However, there is still an urgent need to speed up their translation to clinical practice. Cost-effective and industrially scalable methodologies are still needed. Herein, the production of vitamin C-loaded liposomes for nutraceutical purposes has been investigated and optimized by adopting a High-Pressure Homogenizer. Initially, the impact of process parameters on particles size, distributions, and morphology was explored. The findings document that the pressure and cycle manipulation allow for control over liposome size and polydispersity, reaching a maximum encapsulation efficiency exceeding 80%. This significantly improves the storage stability of vitamin C, as demonstrated by monitoring its antioxidant activity. Furthermore, the in vitro simulation of gastrointestinal digestion shows that liposomes could protect the active substance from damage and control its release in the gastrointestinal fluid. Thus, the whole nanodelivery system can contribute to enhancing vitamin C bioavailability. In conclusion, the results indicate that this innovative approach to producing vitamin C liposomes holds promise for clinical translation and industrial scale-up. Indeed, by utilizing food-grade materials and straightforward equipment, it is possible to produce stable and functional liposomes suitable for health products.
A Light-Powered Micropump with Dynamic Collective Behavior for Reparation
Authors: Sun Yunyu , Wang Hao , Jiang Jiwei , Zhang Hui , Liu Limei , Zhang Keying , Song Bo , Dong Bin
Published: March 2024

Inspired by the collective behaviors of active systems in nature, the collective behavior of micromotors has attracted more and more attention in recent years. However, little attention has been paid to the collective behavior of the immobilized micromotor, i.e., the micropump. In this paper, a unique pentacene-based micropump is reported, which demonstrates dynamic collective behavior activated by white light irradiation. The light irradiation may generate the photochemical reactions between pentacene and water, leading to the electroosmotic flow. As a result, this micropump is capable of pumping the surrounding solution inward along the substrate surface based on the electroosmosis mechanism. Intriguingly, the inward pumping causes the agglomeration of the tracer particles on the surface of the micropump. In addition, the aggregation can migrate following the change in the light irradiation position between two adjacent micropumps. Based on the aggregating and migrating behaviors of this pentacene-based micropump, we have achieved the conductivity restoration of the cracked circuit.
Optical Analysis of Perovskite III-V Nanowires Interpenetrated Tandem Solar Cells
Authors: Tirrito Matteo , Manley Phillip , Becker Christiane , Unger Eva , Borgström Magnus T.
Published: March 2024

Multi-junction photovoltaics approaches are being explored to mitigate thermalization losses that occur in the absorption of high-energy photons. However, the design of tandem cells faces challenges such as light reflection and parasitic absorption. Nanostructures have emerged as promising solutions due to their anti-reflection properties, which enhances light absorption. III-V nanowires (NWs) solar cells can achieve strong power conversion efficiencies, offering the advantage of potentially integrating tunnel diodes within the same fabrication process. Metal halide perovskites (MHPs) have gained attention for their optoelectronic attributes and cost-effectiveness. Notably, both material classes allow for tunable bandgaps. This study explores the integration of MHPs with III-V NWs solar cells in both two-terminal and three-terminal configurations. Our primary focus lies in the optical analysis of a tandem design using III-V semiconductor nanowire arrays in combination with perovskites, highlighting their potential for tandem applications. The space offered by the compact footprint of NW arrays is used in an interpenetrated tandem structure. We systematically optimize the bottom cell, addressing reflectivity and parasitic absorption, and extend to a full tandem structure, considering experimentally feasible thicknesses. Simulation of a three-terminal structure highlights a potential increase in efficiency, decoupling the operating points of the subcells. The two-terminal analysis underscores the benefits of nanowires in reducing reflection and achieving a higher matched current between the top and the bottom cells. This research provides significant insights into NW tandem solar cell optics, enhancing our understanding of their potential to improve photovoltaic performance.
UV Nanoimprint Lithography—Impact of Coating Techniques on Pattern Quality
Authors: Rimböck Johanna , Schuster Patrick , Vsetecka Lisa , Thanner Christine
Published: March 2024

In this work, three different coating techniques are compared and their applicability for ultraviolet nanoimprint lithography (UV-NIL) is investigated. As UV-NIL is considered a suitable volume manufacturing production solution for various emerging applications, it is mandatory to consider environmental aspects such as operational energy use and material consumption as well as waste management. In this paper, spin coating, spray coating, and inkjet coating are used to coat both a high refractive index resin (n = 1.9) and a filler-free resin (n = 1.5), respectively. Variable Angle Spectroscopy Ellipsometry (VASE) was used to analyze the influence of different process parameters on the resin thickness as well as to compare the refractive index achieved from each coating technology. Finally, the applicability of the different coating methods for UV-NIL was investigated by imprinting the resin layers with different test structures. For the final imprints, the resolution, the surface roughness, and the pattern fidelity over 25 imprints was assessed using AFM. Finally, a comparison of the resin consumption and the process time was performed for each coating method.
Healthful Eating Behaviors among Couples Contribute to Lower Gestational Weight Gain
Authors: Sparks Joshua R. , Redman Leanne M. , Drews Kimberly L. , Sims Clark R. , Krukowski Rebecca A. , Andres Aline
Published: March 2024

Through longitudinal analysis from the GLOWING cohort study, we examined the independent and joint relationships between couples’ eating behaviors and gestational weight gain (GWG). Pregnant persons (n = 218) and their non-pregnant partners (n = 157) completed an Eating Inventory. GWG was calculated as gestation weight at 36 weeks minus that at 10 weeks. General linear models were used to examine the relationships between GWG and the pregnant persons, non-pregnant partners, and couples (n = 137; mean of pregnant persons and non-pregnant partners) cognitive restraint (range 0–21), dietary disinhibition (range 0–18), and perceived hunger (range 0–14), with higher scores reflecting poorer eating behaviors. The adjusted models included race/ethnicity, education, income, marital status, and age. The pregnant persons and their non-pregnant partners’ cognitive restraint, dietary disinhibition, and perceived hunger scores were 9.8 ± 4.7, 4.8 ± 3.2, and 4.4 ± 2.5 and 6.6 ± 4.6, 5.4 ± 3.4, and 4.7 ± 3.2, respectively. Higher cognitive restraint scores among the pregnant persons and couples were positively associated with GWG (p ≤ 0.04 for both). Stratified analyses revealed this was significant for the pregnant persons with overweight (p ≤ 0.04). The non-pregnant partners’ eating behaviors alone were not significantly associated with GWG (p ≥ 0.31 for all). The other explored relationships between GWG and the couples’ eating behaviors were insignificant (p ≥ 0.12 for all). Among the pregnant persons and couples, reduced GWG may be achieved with higher levels of restrained eating. Involving non-pregnant partners in programs to optimize GWG may be beneficial.
Barriers to Adopting a Plant-Based Diet in High-Income Countries: A Systematic Review
Authors: Rickerby Alice , Green Rosemary
Published: March 2024

Adopting a plant-based diet (PBD) has been shown to reduce the risk of developing certain diseases and is linked to environmental benefits. This review synthesises the evidence on the barriers adults aged 18 to 65 living in high-income countries (HIC) may experience when adopting a PBD. A systematic literature review was conducted using four search databases: Medline, Embase, Global Health, and Web of Science. Barriers were mapped to behaviour change strategies using the COM-B model. Ten studies were included in the final review, including 1740 participants. Five were qualitative, four were cross-sectional, and one was a pre- and-post-intervention study. In total, 40 barriers were identified and synthesised into 11 themes: financial, lack of knowledge, emotional, health, convenience, social, enjoyment of meat, environmental, accessibility, personal ability, and media. Of the 40 barriers, nutritional intake/requirements (categorised into the “health” theme) had the most evidence. This barrier encompassed concerns around being able to meet nutritional needs if an individual were to adopt a PBD. Habits (in the “personal ability” theme), which included established eating habits and habitual behaviours relating to animal-source foods, had the second most evidence alongside the barrier of not knowing what to eat as part of a PBD (in the “lack of knowledge” theme). Education interventions and communication/marketing policies were the behaviour change mechanisms mapped onto these barriers. Future interventions should focus on informing individuals about what to consume as part of a nutritionally balanced PBD and facilitating habitual dietary change.
The Use of Three-Dimensional Images and Food Descriptions from a Smartphone Device Is Feasible and Accurate for Dietary Assessment
Authors: Schenk Jeannette M. , Boynton Alanna , Kulik Pavel , Zyuzin Alexei , Neuhouser Marian L. , Kristal Alan R.
Published: March 2024

Technology-assisted dietary assessment has the potential to improve the accuracy of self-reported dietary intake. This study evaluates MealScan3D (MS3D), a mobile device-based food recording system, which uses three-dimensional images to obtain food volumes and an application to capture algorithm-driven food intake data. Participants (n = 179) were randomly assigned and trained to record three meals using either MS3D or a written food record (WFR). Generous amounts of standardized meals were provided, and participants self-selected portions for each food. The weights of provided and uneaten/leftover foods were used to determine true intake. For total energy intake (three meals combined), validity (Pearson correlation) was significantly higher for MS3D vs. the WFR (p < 0.001); when interpreted as the percentage of variance in energy intake explained, MS3D explained 84.6% of true variance, a 25.3% absolute and 42.6% relative increase over the 59.3% explained by the WFR. For 9 of 15 individual foods, the Pearson correlations between true and reported portion size estimates were significantly larger for MS3D than the WFR. Bias was smaller (intercepts were closer to the means) for 9 of 15 foods and the regression coefficients for 10 of 15 foods were significantly closer to 1.0 in the MS3D arm. MS3D is feasible for dietary assessment and may provide improvements in accuracy compared to WFRs.
Beyond the Tropics: Unraveling the Complex Relationship between Sun Exposure, Dietary Intake, and Vitamin D Deficiency in Coastal Malaysians
Authors: Gill Shameena , Adenan Alia Maisara , Thomas Emillia Elza , Haleelur Rahman Arifah , Rahim Noor Baitee A. , Ismail Noor Akmal Shareela
Published: March 2024

Despite Malaysia’s year-round sunny climate, vitamin D deficiency is surprisingly common among Malaysians. However, we hypothesise that vitamin D levels among coastal populations are above average. Thus, we aim to investigate vitamin D levels and correlate them with the potential contributing factors from three selected coastal villages in Johor, Melaka, and Negeri Sembilan. Convenient sampling was employed to recruit 120 Malay male and female participants, and dried blood spots (DBS) were obtained to measure 25 (OH) vitamin D3 levels via immunoassay. Participants also completed two questionnaires: the Sun Exposure and Protection Index (SEPI) and a validated food frequency questionnaire for Malaysians. The participant pool comprised 35.20% males and 64.80% females who completed all questionnaires and underwent DBS sampling. Our analysis revealed a significant difference (p < 0.05) based on skin tones, impacting various facets of the SEPI, including sunscreen usage, protective clothing utilisation, and the adoption of protective headwear. Furthermore, gender emerged as another pivotal factor, demonstrating significant distinctions in these SEPI components. Nevertheless, there is a weak correlation between SEPI scores and vitamin D levels. Subsequent regression analysis did produce statistically significant results (p = 0.018), yet the associated low R2 value indicated a weak correlation between dietary vitamin D intake that impacts vitamin D levels. In conclusion, our preliminary findings indicate that sun exposure and dietary factors are not the sole determinants of 25-OH vitamin D3 levels. However, we require more samples from various coastal locations for a definitive justification.
Comprehensive Review of Functional and Nutraceutical Properties of Craterellus cornucopioides (L.) Pers.
Authors: Bumbu Mariana-Gabriela , Niculae Mihaela , Ielciu Irina , Hanganu Daniela , Oniga Ilioara , Benedec Daniela , Nechita Mihaela-Ancuța , Nechita Vlad-Ionuț , Marcus Ioan
Published: March 2024

Nutraceuticals represent an emerging and dynamic scientific field due to their important potential in integrated healthcare through nutritional and medicinal approaches that interact and complement each other mutually. In an attempt to find new sources for such preparations, the present research focuses on the species Craterellus cornucopioides (L.) Pers. (Cantharellaceae), also known as the black trumpet. This wild mushroom species is renowned for its culinary excellence and unique taste and is used especially in a dehydrated state. However, beyond its gastronomic value, recent scientific investigations have revealed its potential as a source of bioactive compounds with pharmaceutical and therapeutic significance. Our study aimed, therefore, to review the current data regarding the morphology, chemical profile, and medicinal potential of the black trumpet mushroom, highlighting its unique attributes. By conducting a comprehensive literature analysis, this paper contributes to the broader understanding of this remarkable fungal species as a potential functional food and its promising applications in the field of therapeutics.
Oxygen: Highlights from the Papers Published in the Journal up to February 2024
Authors: Hancock John T.
Published: March 2024

Oxygen (O2) was discovered approximately 250 years ago (Contribution 1), a breakthrough accredited to at least three people: Antoine-Laurent de Lavoisier in France (Antoine Lavoisier), Carl Wilhelm Scheele in Sweden, and Joseph Priestley in England [...]
Cellular Uptake and Transport Mechanism Investigations of PEGylated Niosomes for Improving the Oral Delivery of Thymopentin
Authors: Liu Mengyang , Svirskis Darren , Proft Thomas , Loh Jacelyn , Huang Yuan , Wen Jingyuan
Published: March 2024

Background: Although its immunomodulatory properties make thymopentin (TP5) appealing, its rapid metabolism and inactivation in the digestive system pose significant challenges for global scientists. PEGylated niosomal nanocarriers are hypothesized to improve the physicochemical stability of TP5, and to enhance its intestinal permeability for oral administration. Methods: TP5-loaded PEGylated niosomes were fabricated using the thin film hydration method. Co-cultured Caco-2 and HT29 cells with different ratios were screened as in vitro intestinal models. The cytotoxicity of TP5 and its formulations were evaluated using an MTT assay. The cellular uptake and transport studies were investigated in the absence or presence of variable inhibitors or enhancers, and their mechanisms were explored. Results and Discussion: All TP5 solutions and their niosomal formulations were nontoxic to Caco-2 and HT-29 cells. The uptake of TP5-PEG-niosomes by cells relied on active endocytosis, exhibiting dependence on time, energy, and concentration, which has the potential to significantly enhance its cellular uptake compared to TP5 in solution. Nevertheless, cellular transport rates were similar between TP5 in solution and its niosomal groups. The cellular transport of TP5 in solution was carried out mainly through MRP5 endocytosis and a passive pathway and effluxed by MRP5 transporters, while that of TP5-niosomes and TP5-PEG-niosomes was carried out through adsorptive- and clathrin-mediated endocytosis requiring energy. The permeability and transport rate was further enhanced when EDTA and sodium taurocholate were used as the penetration enhancers. Conclusions: This research has illustrated that PEG-niosomes were able to enhance the cellular uptake and maintain the cellular transport of TP5. This study also shows this formulation’s potential to serve as an effective carrier for improving the oral delivery of peptides.
Saturated Gain-Induced Non-Reciprocal Transmission and Broadband On-Chip Optical Isolator
Authors: Xue Mingyuan , Tong Haojiang , Dong Hao , Wang Meijia
Published: March 2024

To overcome the limitation of dynamic reciprocity, a new method for designing broadband on-chip optical isolators is proposed and demonstrated based on saturated gain, which is able to support simplex and duplex operation modes. By connecting a saturated gain waveguide to an appropriate linear loss waveguide, broadband isolation is predicted and proved theoretically through saturated gain-induced non-reciprocal transmission. The proposed isolator is numerically demonstrated with an operating band of 59 nm and an isolation ratio of −20 dB at the central wavelength of 1550 nm. It is noteworthy that when the current pump changes, the isolator still works well and keeps the high isolation ratio at a different input power. The footprint of the whole device is 465 μm × 0.35 μm which satisfies the requirement of photonic integrated circuits. The proposed isolator, with the combined advantages of compact footprint, broadband, duplex operation and high isolation, can enable on-chip unidirectional transmission and complex topological routing designation.
Folk Knowledge and Perceptions about the Use of Wild Fruits and Vegetables–Cross-Cultural Knowledge in the Pipli Pahar Reserved Forest of Okara, Pakistan
Authors: Jabeen Sadia , Arshad Fahim , Harun Nidaa , Waheed Muhammad , Alamri Saud , Haq Shiekh Marifatul , Vitasović-Kosić Ivana , Fatima Kaneez , Chaudhry Abdul Shakoor , Bussmann Rainer W.
Published: March 2024

Wild fruits and vegetables (WFVs) have been vital to local communities for centuries and make an important contribution to daily life and income. However, traditional knowledge of the use of wild fruits is at risk of being lost due to inadequate documentation. This study aimed to secure this knowledge through intermittent field visits and a semi-structured questionnaire. Using various ethnobotanical data analysis tools and SPSS (IBM 25), this study identified 65 WFV species (52 genera and 29 families). These species, mostly consumed as vegetables (49%) or fruits (43%), were predominantly herbaceous (48%) in wild and semi-wild habitats (67%). 20 WFVs were known to local communities (highest RFC), Phoenix sylvestris stood out as the most utilized species (highest UV). Surprisingly, only 23% of the WFVs were sold at markets. The survey identified 21 unique WFVs that are rarely documented for human consumption in Pakistan (e.g., Ehretia obtusifolia, Euploca strigosa, Brassica juncea, Cleome brachycarpa, Gymnosporia royleana, Cucumis maderaspatanus, Croton bonplandianus, Euphorbia prostrata, Vachellia nilotica, Pongamia pinnata, Grewia asiatica, Malvastrum coromandelianum, Morus serrata, Argemone mexicana, Bambusa vulgaris, Echinochloa colonum, Solanum virginianum, Physalis angulata, Withania somnifera, Zygophyllum creticum, and Peganum harmala), as well as 14 novel uses and five novel edible parts. Despite their ecological importance, the use of WFVs has declined because local people are unaware of their cultural and economic value. Preservation of traditional knowledge through education on conservation and utilization could boost economies and livelihoods in this and similar areas worldwide.
Antibacterial Ingredients and Modes of the Methanol-Phase Extract from the Fruit of Amomum villosum Lour.
Authors: Zhang Kaiyue , Cao Fengfeng , Zhao Yueliang , Wang Hengbin , Chen Lanming
Published: March 2024

Epidemics of infectious diseases threaten human health and society stability. Pharmacophagous plants are rich in bioactive compounds that constitute a safe drug library for antimicrobial agents. In this study, we have deciphered for the first time antibacterial ingredients and modes of the methanol-phase extract (MPE) from the fruit of Amomum villosum Lour. The results have revealed that the antibacterial rate of the MPE was 63.64%, targeting 22 species of common pathogenic bacteria. The MPE was further purified by high performance liquid chromatography (Prep-HPLC), and three different constituents (Fractions 1–3) were obtained. Of these, the Fraction 2 treatment significantly increased the cell membrane fluidity and permeability, reduced the cell surface hydrophobicity, and damaged the integrity of the cell structure, leading to the leakage of cellular macromolecules of Gram-positive and Gram-negative pathogens (p < 0.05). Eighty-nine compounds in Fraction 2 were identified by ultra HPLC-mass spectrometry (UHPLC-MS) analysis, among which 4-hydroxyphenylacetylglutamic acid accounted for the highest 30.89%, followed by lubiprostone (11.86%), miltirone (10.68%), and oleic acid (10.58%). Comparative transcriptomics analysis revealed significantly altered metabolic pathways in the representative pathogens treated by Fraction 2 (p < 0.05), indicating multiple antibacterial modes. Overall, this study first demonstrates the antibacterial activity of the MPE from the fruit of A. villosum Lour., and should be useful for its application in the medicinal and food preservative industries against common pathogens.
Infrared Thermography Monitoring of Durum and Common Wheat for Adaptability Assessing and Yield Performance Prediction
Authors: Rippa Massimo , Di Mola Ida , Ottaiano Lucia , Cozzolino Eugenio , Mormile Pasquale , Mori Mauro
Published: March 2024

Wheat is one of the most cultivated cereals thanks to both its nutritional value and its versatility to technological transformation. Nevertheless, the growth and yield of wheat, as well as of the other food crops, can be strongly limited by many abiotic and biotic stress factors. To face this need, new methodological approaches are required to optimize wheat cultivation from both a qualitative and quantitative point of view. In this context, crop analysis based on imaging techniques has become an important tool in agriculture. Thermography is an appealing method that represents an outstanding approach in crop monitoring, as it is well suited to the emerging needs of the precision agriculture management strategies. In this work, we performed an on-field infrared monitoring of several durum and common wheat varieties to evaluate their adaptability to the internal Mediterranean area chosen for cultivation. Two new indices based on the thermal data useful to estimate the agronomical response of wheat subjected to natural stress conditions during different phenological stages of growth have been introduced. The comparison with some productive parameters collected at harvest highlighted the correlation of the indices with the wheat yield (ranging between p < 0.001 and p < 0.05), providing interesting information for their early prediction.
Influence of Liner Surface with Parameterized Pit Texture on the Friction Characteristics of Piston Rings
Authors: Zhang Hongyang , Gong Junzhen , Liu Xiaori , Sun Wen , Sun Ke , Bai Shuzhan
Published: March 2024

The arrangement of a pit-shaped surface texture on the surface of a cylinder liner significantly affects reductions in piston ring friction, and the influence of the structural parameters and spatial distribution on piston ring friction power consumption is unclear. In this paper, the diameter, depth, axial spacing distance, and radial spacing distance of the pits on the inner surface of a cylinder liner were used as variable parameters to process the surface textures of different schemes, and then a friction and wear test was carried out on UMT piston ring–cylinder liner specimens, several texture schemes with the best anti-friction effect were selected, an engine bench test was carried out by comparing these texture schemes with non-texture schemes, and the frictional torque and fuel consumption of the engine were studied at different oil temperatures. The results show that the depth of the pits in the surface texture of a cylinder liner has a greater influence on the friction reduction effect, followed by the radius. The higher the oil temperature in the engine bench test, the greater the impact of the surface texture. The reduction in fuel consumption was greater in the lower-speed region after structuring the textured cylinder liner compared to the non-textured cylinder liner. Specifically, the friction coefficient was mainly affected by the depth of the pits, and the depths of the pits in the texture schemes with good friction reduction effect were all 17–19 μm. The best friction reduction could be achieved when the pit radius is around 50 μm, with little difference in pit depth. When the oil temperature was 95 °C, the average drag torque reduction was about 1.69 Nm; when the oil temperature was 105 °C, the decrease was about 2.54 Nm; and when the oil temperature was 105 °C, the decrease was about 4.53 Nm. After adding the surface texture of the cylinder liner, the fuel consumption rate of the engine equipped with the structured cylinder liner was generally reduced compared with that of the original cylinder liner engine. Among them, the average and subsequent consumption rate of surface assembly scheme 11 decreased the most, with a value of 1.3 g/kwh.
Microwave-Assisted Synthesis of 5-Substituted 3-Amino-1,2,4-triazoles from Aminoguanidine Bicarbonate and Carboxylic Acids
Authors: Gümüş Mustafa Kemal , Gorobets Mykola Yu. , Uludag Nesimi
Published: March 2024

The effect of the molar ratio between reagents, reaction time and temperature on the yield of 5-substituted 3-amino-1,2,4-triazoles obtained by the direct condensation of carboxylic acids with aminoguanidine bicarbonate under acid catalysis conditions was studied. As a result, a general green straightforward synthesis of the title compounds bearing aliphatic substituents or a phenyl ring was developed using sealed reaction vials under controlled microwave synthesis conditions that are suitable for the application of volatile starting carboxylic acids. Our straightforward synthetic method proposed in this work increases the synthetic accessibility of these widely used building blocks and therefore is able to significantly expand the structural diversity of compounds containing a triazole moiety for the needs of drug discovery.
Research Progress on the Dynamic Stability of Dry Gas Seals
Authors: Zhang Lanxia , Ding Xuexing , Wang Shipeng , Zhang Shuai , Ding Junhua
Published: March 2024

Concerning the application of high-precision, enormous rotating equipment under harsh working conditions, the advantages of dry gas sealing technology are increasingly obvious. Herein, research on the dynamic stability of dry gas seals is reviewed based upon their operating mechanisms. The influence of the dry gas seal structure, vibration response, and dynamic followability on the reliability of the shaft end sealing system of rotating machinery is the focus of current dry gas sealing technology. This work reviews the research history; analyzes the key coefficient of the instability of the sealing system under external disturbances, and the existing research on stability models; discusses the influence of starting and stopping characteristics, working conditions, and groove parameters on the stability of dry gas seals; and points out the shortcomings in the existing research. In addition, potential developments in dynamic stability are proposed, including improving model accuracy, improving experimental techniques, or applying intelligent control and optimization methods to enhance the dynamic stability of the sealing system. Finally, the development prospects for dry gas sealing technology in intelligent monitoring and wide temperature range adaptations are discussed, and theoretical guidance for improving a dry gas seal system is provided.
Physico-Chemical Characterization of Encapsulated Fennel Essential Oil under the Influence of Spray-Drying Conditions
Authors: Repajić Maja , Elez Garofulić Ivona , Marčac Duraković Nina , Balun Marta , Cegledi Karla , Cegledi Ena , Dobroslavić Erika , Dragović-Uzelac Verica
Published: March 2024

In this study, fennel essential oil (EO) was spray-dried, varying the wall material type (two-component blends of maltodextrin (MD), β-cyclodextrin (β-CD) and gum arabic (GA)), the wall material ratio (1:1, 1:3 and 3:1) and the drying temperature (120, 160 and 200 °C). A total of 27 powders were analyzed for their moisture content, solubility, hygroscopicity, bulk density and particle size, while powder recovery and oil retention were determined in terms of encapsulation efficiency. The morphology and chemical composition of the powder obtained under optimal conditions were additionally analyzed by scanning electron microscopy and gas chromatography-mass spectrometry. The results showed that all of the powders had generally good properties, exhibiting a low moisture content, high powder recovery and high oil retention. A 1:3 MD:GA mixture and a drying temperature of 200 °C were found to be optimal for the spray-drying of fennel EO, producing a powder with a low moisture content (3.25%) and high solubility (56.10%), while achieving a high powder recovery (72.66%) and oil retention (72.11%). The chemical profiles of the initial and encapsulated fennel EO showed quantitative differences without qualitative changes, with an average 24.2% decrease in the volatiles in the encapsulated EO. Finally, spray-drying proved to be a successful tool for the stabilization of fennel EO, at the same time expanding the possibilities for its further use.
Quick Extraction of Joint Surface Attitudes and Slope Preliminary Stability Analysis: A New Method Using Unmanned Aerial Vehicle 3D Photogrammetry and GIS Development
Authors: Li Qiyu , Yao Xin , Li Renjiang , Zhou Zhenkai , Yao Chuangchuang , Ren Kaiyu
Published: March 2024

The present study proposes a preliminary analysis method for rock mass joint acquisition, analysis, and slope stability assessment based on unmanned aerial vehicle (UAV) photogrammetry to extract the joint surface attitude in Geographic Information Systems (GIS). The method effectively solves the difficulties associated with the above issues. By combining terrain-following photogrammetry (TFP) and perpendicular and slope surface photogrammetry (PSSP), the three-dimensional (3D) information can be efficiently obtained along the slope characteristics’ surface, which avoids the information loss involved in traditional single-lens aerial photography and the information redundancy of the five-eye aerial photography. Then, a semi-automatic geoprocessing tool was developed within the ArcGIS Pro 3.0 environment, using Python for the extraction of joint surfaces. Multi-point fitting was used to calculate the joint surface attitude. The corresponding attitude symbols are generated at the same time. Finally, the joint surface attitude information is used to perform stereographic projection and kinematic analysis. The former can determine the dominant joint group, and the latter can obtain the probability of four types of failure, including planar sliding, wedge sliding, flexural toppling, and direct toppling. The integrated stability evaluation method studied in this paper, which combines a 3D interpretation of UAV and GIS stereographic projection statistical analysis, has the advantages of being efficient and user-friendly, and requires minimal prior knowledge. The results can aid in the geological surveys of slopes and guide engineering practices.
Enhanced Seamless Video Fusion: A Convolutional Pyramid-Based 3D Integration Algorithm
Authors: Zhang Yueheng , Yuan Jing , Yan Changxiang
Published: March 2024

Video fusion aims to synthesize video footage from different sources into a unified, coherent output. It plays a key role in areas such as video editing and special effects production. The challenge is to ensure the quality and naturalness of synthetic video, especially when dealing with footage of different sources and qualities. Researchers continue to strive to optimize algorithms to adapt to a variety of complex application scenarios and improve the effectiveness and applicability of video fusion. We introduce an algorithm based on a convolution pyramid and propose a 3D video fusion algorithm that looks for the potential function closest to the gradient field in the least square sense. The 3D Poisson equation is solved to realize seamless video editing. This algorithm uses a multi-scale method and wavelet transform to approximate linear time. Through numerical optimization, a small core is designed to deal with large target filters, and multi-scale transformation analysis and synthesis are realized. In terms of seamless video fusion, it shows better performance than existing algorithms. Compared with editing multiple 2D images into video after Poisson fusion, the video quality produced by this method is very close, and the computing speed of the video fusion is improved to a certain extent.
Point Cloud Registration Method Based on Geometric Constraint and Transformation Evaluation
Authors: Kang Chuanli , Geng Chongming , Lin Zitao , Zhang Sai , Zhang Siyao , Wang Shiwei
Published: March 2024

Existing point-to-point registration methods often suffer from inaccuracies caused by erroneous matches and noisy correspondences, leading to significant decreases in registration accuracy and efficiency. To address these challenges, this paper presents a new coarse registration method based on a geometric constraint and a matrix evaluation. Compared to traditional registration methods that require a minimum of three correspondences to complete the registration, the proposed method only requires two correspondences to generate a transformation matrix. Additionally, by using geometric constraints to select out high-quality correspondences and evaluating the matrix, we greatly increase the likelihood of finding the optimal result. In the proposed method, we first employ a combination of descriptors and keypoint detection techniques to generate initial correspondences. Next, we utilize the nearest neighbor similarity ratio (NNSR) to select high-quality correspondences. Subsequently, we evaluate the quality of these correspondences using rigidity constraints and salient points’ distance constraints, favoring higher-scoring correspondences. For each selected correspondence pair, we compute the rotation and translation matrix based on their centroids and local reference frames. With the transformation matrices of the source and target point clouds known, we deduce the transformation matrix of the source point cloud in reverse. To identify the best-transformed point cloud, we propose an evaluation method based on the overlap ratio and inliers points. Through parameter experiments, we investigate the performance of the proposed method under various parameter settings. By conducting comparative experiments, we verified that the proposed method’s geometric constraints, evaluation methods, and transformation matrix computation consistently outperformed other methods in terms of root mean square error (RMSE) values. Additionally, we validated that our chosen combination for generating initial correspondences outperforms other descriptor and keypoint detection combinations in terms of the registration result accuracy. Furthermore, we compared our method with several feature-matching registration methods, and the results demonstrate the superior accuracy of our approach. Ultimately, by testing the proposed method on various types of point cloud datasets, we convincingly established its effectiveness. Based on the evaluation and selection of correspondences and the registration result’s quality, our proposed method offers a solution with fewer iterations and higher accuracy.
Sensors for Robots
Authors: Zhao Xin , Sun Mingzhu , Zhao Qili
Published: March 2024

Currently, robots are playing significant roles in industry [...]
Using Surrogate Parameters to Enhance Monitoring of Community Wastewater Management System Performance for Sustainable Operations
Authors: Shi Zhining , Chow Christopher W. K. , Gao Jing , Xing Ke , Liu Jixue , Li Jiuyong
Published: March 2024

Community wastewater management systems (CWMS) are small-scale wastewater treatment systems typically in regional and rural areas with less sophisticated treatment processes and often managed by local governments or communities. Research and industrial applications have demonstrated that online UV-Vis sensors have great potential for improving wastewater monitoring and treatment processes. Existing studies on the development of surrogate parameters with models from spectral data for wastewater were largely limited to lab-based. In contrast, industrial applications of these sensors have primarily targeted large wastewater treatment plants (WWTPs), leaving a gap in research for small-scale WWTPs. This paper demonstrates the suitability of using a field-based online UV-Vis sensor combined with advanced data analytics for CWMSs as an early warning for process upset to support sustainable operations. An industry case study is provided to demonstrate the development of surrogate monitoring parameters for total suspended solids (TSSs) and chemical oxygen demand (COD) using the UV-Vis spectral data from an online UV-Vis sensor. Absorbances at a wavelength of 625 nm (UV625) and absorbances at a wavelength of 265 nm (UV265) were identified as surrogate parameters to measure TSSs and COD, respectively. This study contributes to the improvement of WWTP performance with a continuous monitoring system by developing a process monitoring framework and optimization strategy.