bluuebunny/crossref_metadata_2025 huggingface dataset:
Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation
Authors: Atkinson Eliza , Tuza Zoltan , Perrino Giansimone , Stan Guy-Bart , Ledesma-Amaro Rodrigo
Published: June 2022

Background

Low-cost sustainable feedstocks are essential for commercially viable biotechnologies. These feedstocks, often derived from plant or food waste, contain a multitude of different complex biomolecules which require multiple enzymes to hydrolyse and metabolise. Current standard biotechnology uses monocultures in which a single host expresses all the proteins required for the consolidated bioprocess. However, these hosts have limited capacity for expressing proteins before growth is impacted. This limitation may be overcome by utilising division of labour (DOL) in a consortium, where each member expresses a single protein of a longer degradation pathway.

Results

Here, we model a two-strain consortium, with one strain expressing an endohydrolase and a second strain expressing an exohydrolase, for cooperative degradation of a complex substrate. Our results suggest that there is a balance between increasing expression to enhance degradation versus the burden that higher expression causes. Once a threshold of burden is reached, the consortium will consistently perform better than an equivalent single-cell monoculture.

Conclusions

We demonstrate that resource-aware whole-cell models can be used to predict the benefits and limitations of using consortia systems to overcome burden. Our model predicts the region of expression where DOL would be beneficial for growth on starch, which will assist in making informed design choices for this, and other, complex-substrate degradation pathways.
Nanoparticles mediated tumor microenvironment modulation: current advances and applications
Authors: Raju Ganji Seeta Rama , Pavitra Eluri , Varaprasad Ganji Lakshmi , Bandaru Sai Samyuktha , Nagaraju Ganji Purnachandra , Farran Batoul , Huh Yun Suk , Han Young-Kyu
Published: June 2022

The tumor microenvironment (TME) plays a key role in cancer development and emergence of drug resistance. TME modulation has recently garnered attention as a potential approach for reprogramming the TME and resensitizing resistant neoplastic niches to existing cancer therapies such as immunotherapy or chemotherapy. Nano-based solutions have important advantages over traditional platform and can be specifically targeted and delivered to desired sites. This review explores novel nano-based approaches aimed at targeting and reprogramming aberrant TME components such as macrophages, fibroblasts, tumor vasculature, hypoxia and ROS pathways. We also discuss how nanoplatforms can be combined with existing anti-tumor regimens such as radiotherapy, immunotherapy, phototherapy or chemotherapy to enhance clinical outcomes in solid tumors.
Preliminary evaluation of the Chinese version of the patient-reported outcomes measurement information system 29-item profile in patients with aortic dissection
Authors: Huang Wanbing , Wu Qiansheng , Zhang Yufen , Tian Chong , Huang Haishan , Huang Sufang , Zhou Yanrong , He Jing , Wang Hui
Published: June 2022

Background

The Patient-Reported Outcomes Measurement Information System 29-item Profile (PROMIS-29) has been widely used to measure health outcomes from the patient’s perspective. It has not been validated in adults with aortic disease. The aim of this study was to explore the reliability and validity of the Chinese PROMIS-29 among patients undergoing surgery for aortic dissection (AD).

Methods

A cross-sectional design was applied. Eligible patients completed a questionnaire that contained the PROMIS-29 and legacy measures, including the Short Form-12 Health Survey (SF-12), 8-item Somatic Symptom Scale (SSS-8), Generalized Anxiety Disorder–2 (GAD-2), and Patient Health Questionnaire-2 (PHQ-2). The structural validity of the PROMIS-29 was evaluated using confirmatory factor analysis (CFA). Reliability was evaluated with Cronbach’s α. Construct validity was assessed by calculating Spearman’s rank correlations and comparing known-group differences.

Results

In total, a sample of 327 AD patients was included in the final analysis. Most of them were male (89%) with a mean age of 52.7 (± 10.3). CFA revealed good model fit of the seven-factor structure within PROMIS-29, as well as most domains in single-factor analysis. Reliability was confirmed with Cronbach’s α > 0.90. Correlations between comparable domains of the PROMIS-29 and those of legacy questionnaires and most know-group comparisons were observed as hypothesized.

Conclusions

This study found evidence for acceptable structural validity, construct validity and internal consistency of the PROMIS-29 in a sample of AD patients. It can be applied to AD survivors by researchers or clinicians, measuring outcomes after surgery and identifying those with worse health status.
The Multifaceted Melanocortin Receptors
Authors: Laiho Linda , Murray Joanne Fiona
Published: June 2022

The 5 known melanocortin receptors (MCs) have established physiological roles. With the exception of MC2, these receptors can behave unpredictably, and since they are more widely expressed than their established roles would suggest, it is likely that they have other poorly characterized functions. The aim of this review is to discuss some of the less well-explored aspects of the 4 enigmatic members of this receptor family (MC1,3-5) and describe how these are multifaceted G protein–coupled receptors (GPCRs). These receptors appear to be promiscuous in that they bind several endogenous agonists (products of the proopiomelanocortin [POMC] gene) and antagonists but with inconsistent relative affinities and effects. We propose that this is a result of posttranslational modifications that determine receptor localization within nanodomains. Within each nanodomain there will be a variety of proteins, including ion channels, modifying proteins, and other GPCRs, that can interact with the MCs to alter the availability of receptor at the cell surface as well as the intracellular signaling resulting from receptor activation. Different combinations of interacting proteins and MCs may therefore give rise to the complex and inconsistent functional profiles reported for the MCs. For further progress in understanding this family, improved characterization of tissue-specific functions is required. Current evidence for interactions of these receptors with a range of partners, resulting in modulation of cell signaling, suggests that each should be studied within the full context of their interacting partners. The role of physiological status in determining this context also remains to be characterized.
Toward Understanding the Photogenic New Citizen: Performance in Vernacular Photography from the Early Turkish Republic, 1920s–1940s
Authors: Baykan Calafato Özge
Published: June 2022

This portfolio looks at photographic self-representations of Turkish citizens of a newly established nation-state from the 1920s until the 1940s. The article explores the ways in which modern Turkish women and men performed their interpretation of the photogenic in photographic self-representations and how these self-representations negotiated the Kemalists' idea of modern citizenship in the early republican era. The desired selves performed in vernacular photographs were influenced not only by the Kemalist revolution but also by class aspirations of the citizens and wider sociocultural developments of the time. In addition, this portfolio reveals some of the complex circulation networks for photographic exchanges, as also suggested by inscriptions produced at the time, contributing to a deeper understanding as to how people constructed their interpretation of the photographic.
Comparing Lumbar Lordosis in Functional Dance Positions in Collegiate Dancers With and Without Low Back Pain
Authors: Skallerud Abigail , Brumbaugh Aaron , Fudalla Stephanie , Parker Tiffany , Robertson Kristen , Pepin Marie-Eve
Published: June 2022

Limited research has explored the relationship between lumbar lordosis and incidence of low back pain in dancers. The purpose of this study was to investigate the relationship between lumbar lordosis in functional dance positions and low back pain (LBP). Thirty full-time undergraduate ballet and modern collegiate dancers (28 female, 2 male) from three university dance programs participated in this study. Functional lumbar lordosis, hip passive range of motion, and core endurance were measured in a single session. Dancers participated in weekly follow-up surveys over a 4-month period to track the presence and severity of LBP. Primary analysis was performed using independent t-tests between groups who did and did not develop pain. Secondary analyses were performed using Pearson's r correlation. Eleven dancers reported no pain during the follow-up period, 16 reported pain, and three did not respond to follow-up surveys. Lumbar lordosis in right developpé á la seconde and right retiré was significantly greater (p < 0.05) in the group who reported pain compared to the group who reported no pain. Increased hold times in the supine bridge position was shown to be fairly correlated (p < 0.05) to reduced lumbar lordosis in first position (Pearson's r = 0.381), left retiré (Pearson's r = 0.396), and right developpé á la seconde (Pearson's r = 0.365). The results of this study suggest a relationship between increased lumbar lordosis in the retiré and developpé á la seconde dance positions and development of LBP. Increased endurance of posterior core musculature and gluteal muscles was inversely related to the magnitude of lumbar lordosis in some dance-specific positions. The measurement of lumbar lordosis during functional dance positions, particularly those requiring single leg stances, may help identify collegiate dancers with increased risk of developing low back pain.
Κοινωνικές και Ηθικές Πτυχές της Βιομετρίας
Authors: Δεσποτίδης Κωνσταντίνος
Published: June 2022

Με την ραγδαία ανάπτυξη της τεχνολογίας, αναπτύσσεται παράλληλα και η επιστήμη της βιομετρίας. Η βιομετρία είναι η επιστήμη που σχετίζεται με την αναγνώριση και την ταυτοποίηση των ατόμων βάσει των φυσικών ή συμπεριφορικών χαρακτηριστικών τους. Με την σειρά τους, τα βιομετρικά συστήματα επικυρώνουν με ακρίβεια την γνησιότητα αυτών των χαρακτηριστικών των ατόμων με αποτέλεσμα να έχουν πληθώρα εφαρμογών. Ωστόσο, αναφορικά με την διαχείριση των βιομετρικών δεδομένων τίθενται ηθικά και κοινωνικά ζητήματα. Τέτοια παραδείγματα είναι η ιδιωτικότητα, η αυτονομία, η διαφάνεια και ο κοινωνικός αποκλεισμός ατόμων διαφορετικών ομάδων, για παράδειγμα του γηραιότερου ηλικιακού πληθυσμού. Ανάλογα ερωτήματα τίθενται και για το πώς χρησιμοποιούνται τα βιομετρικά δεδομένα των προσφύγων από διάφορους οργανισμούς και κυβερνήσεις οι οποίες έχουν αναβαθμίσει τα συστήματα ταυτοποίησης στα σύνορα, με σκοπό την υψηλότερη ασφάλεια. Συνεπώς, ενώ η επιστήμη της βιομετρίας έχει συντελέσει στην βελτίωση του βιοτικού επιπέδου και της ασφάλειας των ανθρώπων, δύνοντας λύσεις σε πολλά προβλήματα, παραλληλα έχει αποτελέσει και αντικείμενο συζήτησης σχετικά με το αν παραβιάζει τα ανθρώπινα δικαιώματα.
QALYs, DALYs και η αξία της ανθρώπινης ζωής
Authors: Τζερμπίνου Ανθή
Published: June 2022

Η παρούσα μελέτη έχει σαν σκοπό την εις βάθος μελέτη των αξιολογήσεων της υγείας, μέσω των μέτρων που χρησιμοποιούνται σήμερα, για τη μέθοδο λήψης αποφάσεων, στη κατανομή όσων ονομάζονται «σπάνιοι πόροι». Στόχος είναι να δοθεί ιδιαίτερη βαρύτητα στο ποιοτικά σταθμισμένο έτος ζωής, στο αντίστοιχο έτος ζωής με αναπηρία καθώς και στην προθυμία πληρωμής. Η δημοσίευση έχει σκοπό την συνεισφορά στη βέλτιστη κατανόηση των ιδιαίτερων καταστάσεων, που μπορεί να βιώνουν μικρές κοινωνικές ομάδες, στις δυσκολίες που ενδέχεται να αντιμετωπίσουν, προκειμένου να αξιολογήσουν αυτές τις καταστάσεις, αλλά και στην εξέταση του ζητήματος υπό το πρίσμα της βιοηθικής. Με βάση προηγούμενες έρευνες, που θέτουν εν αμφιβόλω την χρήση των μηχανισμών μέτρησης υγείας, όπως το QALY και το DALY, που συνδέονται με τη βελτίωση της υγείας του πληθυσμού εντοπίζοντας μάλιστα επιπρόσθετες προκλήσεις, συμπεραίνεται πως δεν είναι δεοντολογικά αποδεκτό να γίνεται η χρήση των μέτρων αυτών αποκλειστικά, ενώ συνιστάται η συμμετοχή ειδικευμένου προσωπικού σε ζητήματα βιοηθικής, κατά την διαδικασία λήψης αποφάσεων.
Glaciers of the Koryak Highlands: assessment of the state using satellite images and field studies
Authors: Ananicheva Maria D. , Aleinikov Alexander A.
Published: June 2022

The Koryak Highlands are a poorly explored area in terms of glaciation and its connection to climate. The purpose of this work is, using Sentinel-2 images (2019), to assess the state of glaciers at present against the background of climatic trends. Spatial patterns of trends of mean summer and annual temperatures, total precipitation and precipitation for the cold period from 1966 to 2019 were compiled: the temperature trends increase towards the Kamchatka Peninsula, and the mean summer temperature trends increase from 2 to 3°C. Precipitation trends increase from the continent to the coastal zone, with cold season trends changing from 0 to minus 50 mm. As a result, 212 glaciers and 279 rock glaciers were discovered in the Highlands, whereas 112 objects that had previously been recognised were not identified: either there is no glacier, or its area is less than 0.001 km2. The reduction in glacier areas compared to the USSR Glacier Inventory (1960s) varies widely by river basin, from 35 to 74%. The mean present glacier area varies from basin to basin from 0.4 to 0.11 km2. The glaciers are mainly corrie type of the N, NE and NW aspects. The change of average glacier firn line is from several tens of metres to 400–500 m.
Pasiaste serce: Wspomnienia starego piłkarza
Authors: Chruściński Zygmunt
Published: June 2022

Cud nad Wisłą, a w zasadzie przy Kałuży… Po wielu miesiącach starań ukazał się drukiem pamiętnik mojego dziadka, Zygmunta Chruścińskiego ‒ Pasiaste Serce. Wspomnienia starego piłkarza. To uczta dla koneserów ‒ wspomnienia mojego dziadka okraszone mnóstwem pięknych, archiwalnych zdjęć, wycinków i cytatów. Piękny język, unikalne fotografie i wspomnienia oraz wiele innych niespodzianek. Zamiast wstępu i zakończenia dwa moje krótkie opowiadania dotyczące Zygmunta Chruścińskiego, piłkarza Cracovii i reprezentanta Polski, kapitana reprezentacji narodowej, a po wojnie dziennikarza „Echa Krakowa", założyciela i pierwszego redaktora naczelnego „Piłkarza", prekursora „Tempa". Polecam gorąco to wydawnictwo! Jacek Chruściński
Effective safeguarding
Authors: Dixon Sally
Published: June 2022

Sally Dixon discusses the importance of safeguarding in dental practices.
Inside knowledge
Authors: Jackson Josie
Published: June 2022

Josie Jackson speaks to former principal dentist and practice owner Ben Atkins about valuing dental nurses and his dos and don'ts for negotiations.
Turning ambition into a reality
Authors: Jackson Josie
Published: June 2022

Josie Jackson talks to Charlotte Lever about her ambition for career progression.
A unique working environment
Authors: Jackson Josie
Published: June 2022

Josie Jackson talks to Louise Worman and Adelina-Rebecca Tirtopan about working in a paediatric dental practice.
Revisiting the functionally generated path technique: is this an aid to predictable digital occlusal design? Part 2. The technique
Authors: Bereznicki Tom , Patel Rohit , Clark Megan
Published: June 2022

The precision of the occlusal scheme is paramount to the production of a successful fixed restoration(s) and its long-term success. The provision of the correct occlusal scheme is one of the requirements when working conformatively, whether in analogue or digital format. Part 1 highlighted occlusal schemes that provide difficulties using established techniques in arriving at a predictable restoration requiring minimal occlusal adjustments chairside. Part 2 introduces the theory of the functionally generated path (FGP) technique. Two cases involving implant-retained restorations are used to highlight the benefit of adopting this technique into the digital workflow in the provision of occlusally precise restorations.

CPD/Clinical Relevance: This article describes the value of the functionally generated path technique, when working digitally, for recording the occlusion for implant-retained restorations.
Managing early childhood caries with silver diamine fluoride in the COVID-19 context: case report
Authors: Cabrera Luis Raziel Martagón , Pacheco Fabio Gregorio Arriola
Published: June 2022

COVID-19 is challenging modern dentistry, obliging all professionals to look for treatment strategies that are in accordance with health and ethical standards, so as to avoid virus transmission in the dental office. In recent years, paediatric dentistry has benefited from minimally invasive techniques to prevent and treat early childhood caries. Silver diamine fluoride (SDF) is a material that follows this philosophy because it provides professionals preventive and arresting actions for carious lesions in a practical and effective way. The present case details the management of a preschool-age patient who attended the dental office due to an emergency during the pandemic. The patient's emergency was resolved, but due to the diagnosis of severe early childhood caries, other mild and moderate carious, lesions were managed with SDF with the objective of avoiding future pulpal lesion development. The aim of this case is to raise awareness among dentists who treat children to consider the biological benefits of SDF in situations, such as the current contingency, as well as the benefits of mitigating the aesthetic effects thanks to the use of potassium iodide.

CPD/Clinical Relevance: The use of silver diamine fluoride in a paediatric patient with early childhood caries is highlighted.
Neutral zone concept for implant placement in mandibular overdentures: a case report
Authors: AL-Oulabi Ayman , Johari Yanti , Husein Adam , Hassan Akram , Shaari Ramizu , Ariffin Azirrawani
Published: June 2022

The objectives for complete denture fabrication are to provide comfort, function and aesthetics. The availability of implant-assisted overdentures has benefited complete denture wearers; however, the success of the prostheses can be further enhanced when the neutral zone concept is incorporated as a mean to locate the correct position of the implants, as well as the tooth arrangement for optimal outcome of the prescribed prostheses.

CPD/Clinical Relevance: Use of the neutral zone concept to assist implant placement and teeth setting in a severely resorbed edentulous mandible is beneficial.
Nutritional potential of Semele sp. shellfish on estrogen levels and follicle stimulating hormone receptor genes in perimenopausal women
Authors: SJAFARAENAN SJAFARAENAN , UMAR MUHAMMAD RUSLAN , HERWIN HERWIN , ISLAMIYATI ANNA , TUWO MUSTIKA , ZULKIFLI ANDI
Published: June 2022

Abstract. Sjafaraenan, Umar MR, Herwin, Islamiyati A, Tuwo M, Zulkifli A. 2022. Nutritional potential of Semelesp. shellfish on estrogen levels and follicle stimulating hormone receptor genes in perimenopausal women. Biodiversitas 23: 1196-1203. The Bonea villagecommunity that often consume shellfish usually have a high libido and experience menopause abovethe age of 50 years.Therefore, this study aims to determinethe nutritional potential of Semele sp.on the Follicle-Stimulating Hormone Receptor (FSH-R) gene in perimenopausal womenas well asthe levels of estrogen and polymorphisms.The experimental method with a one-group preandpost-test design was used by measuring 10 perimenopausal women respondents aged 40-45 before and after consuming Semele sp. 2 times a week for 2 months. Furthermore, blood samples were collected through the median cubital vein 2-3 mL before and after the treatment, The nutritional measurement of Semele sp. including protein was carried out using the Kjeldhal method, carbohydrates using the Anthrone method, and fat using the gravimetry. Also, the mineral content was determined using Atomic Absorption Spectrophotometry(AAS), while the estrogen levels were assessed with the ELISA technique. The FSH gene was examined using DNA extraction and amplification, electrophoresis, as well as Restriction Fragment Length Polymorphism (RFLP) with the Bsrl enzyme, while thet-test was conducted to examinethe effect of shellfish consumption. The results showed that the consumption of Semele sp.increased estrogen levels significantly, as the Asn680Ser, Asn680Asn, and Ser680Se genotypes were found in each respondent with the FSH-R gene. Based on the results,it was concluded that the regular consumption of Semele sp. increases estrogen levels and is applicable as analternative treatment to delay the incidence of menopause.
Forage productivity in native grasslands of Haharu Sub-district, East Sumba District, Indonesia
Authors: WATUWAYA BOGARTH K. , SYAMSU JASMAL A. , BUDIMAN BUDIMAN , USENG DANIEL
Published: June 2022

Abstract. Watuwaya BK, Syamsu JA, Budiman, Useng D. 2022. Forage productivity in native grasslands of Haharu Sub-district, East Sumba District, Indonesia. Biodiversitas 23: 362-1368. The identification and management of native grasslands are important to ensure the availability of beef cattle feed in smallholder farms. This study aims to identify the existing native grasslands in Haharu Sub-district, East Sumba District, East Nusa Tenggara Province, Indonesia and analyze the botanical composition, biomass and the carrying capacity of the native grasslands. We used the combination of remote sensing approach and field survey to identify and measure the productivity of native grasslands. Sentinel 2A imagery was used to identify the area of existing native grasslands using Supervised Classification - Maximum Likelihood method. Meanwhile, the productivity of native grasslands was measured using the Dry Weight Rank method. The results of remote sensing and spatial analysis showed that the existing area of native grasslands in Haharu Sub-district was 324,10 km2 with an Overall Accuracy of 98.63% and Kappa Accuracy of 0.98. The vegetation analysis showed that the botanical composition of native grasslands consisted of grasses 94.42%, legumes 3.55% and weeds 2.07%. The carrying capacity of the studied grassland was 0.68 AU/ha/month in the wet season. In conclusion, the productivity of existing native grasslands in the wet season is still low and special efforts are needed to improve the quality.
First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes
Authors: SIBERO MADA TRIANDALA , FREDERICK EVAN HANSEL , SABDONO AGUS , WIJAYANTI DIAH PERMATA , PRINGGENIES DELIANIS , RADJASA OCKY KARNA , ZILDA DEWI SESWITA , MURWANI RETNO
Published: June 2022

Abstract. Sibero MT, Frederick EH, Sabdono A, Wijayanti DP, Pringgenies D, Radjasa OK, Zilda DS, Murwani R. 2022. First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes. Biodiversitas 23: 1408-1419. Yeast has been widely utilized in various industries due to its enzyme properties. Therefore, plenty of studies focus on exploring yeast biodiversity from diverse sources. However, there are limited reports regarding marine yeast biodiversity and its potential from Indonesia. This study aimed to isolate and identify the seaweed-associated yeast from Jepara in Central Java and Sepanjang Beach in Gunung Kidul, Yogyakarta, then examined their potential to produce extracellular polysaccharides-degrading enzymes (EPEs). Marine yeasts were isolated using standard marine agar (STD) and potato dextrose agar (PDA). All isolates were characterized by their salinity tolerance, morphology, and species confirmation using DNA barcoding. Three EPEs consisting of agarase, alginate-lyase, and carrageenase were screened using polysaccharides-enriched agar media. A specific agarase encoding gene was detected with specific primers. In total, 21 seaweed-associated yeast were successfully isolated from 6 seaweeds and noted as facultative marine yeast. The DNA barcoding study discovered that these yeasts belonged to 5 genera, consisted of Aureobasidium, Candida, Debaryomyces, Hortaea, and Rhodotorula. Moreover, Candida was noted as the most abundant genus (71.42%). It was noted that Hortaea werneckii MTJ.11 and MTJ.13 were positive for all enzymes; Aureobasidium melanogenum MTGK.31 gave a positive result for agarase and carrageenase; while [Candida] zeylanoides MTGK.23 only exhibited alginate-lyase activity. Unfortunately, none of the primers used to detect the presence of genes encoding polysaccharide-degrading enzymes were successfully amplified.
Reproductive aspects of javaen barb fish, Systomus orphoides in the initial domestication program
Authors: Susatyo Priyo , Lestari Windiariani , SUGIHARTO SUGIHARTO , Chasanah Titi
Published: June 2022

Abstract. Susatyo P, Lestari W, Sugiharto, Chasanah T. 2022. Reproductive aspects of javaen barb fish, Systomus orphoides in the initial domestication program. Biodiversitas 23: 1511-1519. The population of Javaen barb, Systomus orphoides (Valenciennes, 1842) in the Serayu River Central Java has declined, and domestication is one of the most critical conservation initiatives. This research aimed to evaluate the reproductive aspect of javaen barb, Systomus orphoides in an initial domestication program. Furthermore, fish samples were collected from the Serayu River and reared in traditional ponds. The bred broodstocks were injected with artificial hormones before spawning, and several reproductive aspects were measured and analyzed descriptively according to the literature. Fish specimens had gonadosomatic indexes ranging from 11±0.3% to 16.2±0.9%. The values represented all phases of oogenesis and spermatogenesis in females and males. The reproductive hormone titer consisted of 854.85-1058.06 pg/mL of 17ß estradiol, 0.29-0.72 ng/mL of progesterone, 10.87-15.68 mIU/mL of follicle-stimulating hormone, and 4.18-9.92 ng/mL of testosterone. These findings demonstrated that the Serayu River's wild Javaen barb population reached all reproductive phases and that broodstocks can be domesticated in the traditional pond.
Pollution level of Banjaran River, Banyumas District, Indonesia: A study based on the Saprobic Index of periphytic microalgae
Authors: Samudra Sesilia Rani , Fitriadi Ren , Baedowi Muhamad , Sari Lilik Kartika
Published: June 2022

Abstract. Samudra SR, Fitriadi R, Baedowi M, Sari LK. 2022. Pollution level of Banjaran River, Banyumas District, Indonesia: A study based on the Saprobic Index of periphytic microalgae. Biodiversitas 23: 1527-1534. The saprobic index of periphytic microalgae can be used to determine the level of water pollution based on the number and composition of species. Banjaran River is one of the rivers in the Banyumas District, Central Java, Indonesia, which is widely used by the local people for daily activities. The increase of domestic and agricultural activities in the river triggers an increase in the pollutants input into the river. This study aims to determine the status of the waters in the midstream of Banjaran River by using the periphytic microalgae saprobic index as a bioindicator of water quality. Purposive sampling method was used in this study by establishing four stations and five replications at each station. The result showed that the abundance of periphytic microalgae ranged from 7,547-8,733 cells/cm2; the diversity index ranged from 1.64-2.07 (moderately polluted waters); the dominance index ranged from 0.21-0.26 (no particular species of periphytic microalgae dominating the four stations); and saprobic index value of Banjaran River ranged from 1.00 to 1.03 which indicates that Banjaran River belongs to ?-meso/oligo saprobic water. In other words, it was water with a light pollution level. An action to reduce organic pollution is necessary, one of which is by reducing the people's activities such as washing and disposing of household waste into the river so that the condition of Banjaran River can be maintained properly and reduce pollution in its subsequent river flows.
Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization
Authors: Talakua Matheos , Suadi Suadi , Djumanto Djumanto , Setyobudi Eko
Published: June 2022

Abstract. Talakua M, Suadi, Djumanto, Setyobudi E. 2022. Record of Hemiramphus lutkei Valenciennes, 1847 from Seram Sea, Maluku, Indonesia with its molecular characterization. Biodiversitas 23: 1560-1566. Hemiramphus sp. is a fish species widely distributed throughout the world. However, the area distribution of each species differs between regions. Hemiramphus archipelagicus could be found in Seram Sea, Maluku and Ekas Bay, Lombok, Hemiramphus sp. from North Maluku Sea, and H. brasiliensis from the North Minahasa Sulawesi. The identification of Hemiramphus in Indonesia uses mainly the morphological approach. This study aims to determine the morphometric and meristic character and identify the halfbeak collected from the Seram Sea, Maluku, using a molecular approach. A total of 214 fish samples were used to determine the morphometric and meristic characters of halfbeak, following the measurement method that has been developed previously. All samples of halfbeak showed similar morphological characteristics. Identification by direct sequencing of the cytochrome c oxidase subunit I (COI) gene was conducted from selected samples. The halfbeak caught in the Seram Sea had an elongated flat body shape, the dorsal part of the body is blue-green, the tip of the lower beak is sharp, enlarged towards the base in the oral cavity; the lower beak is reddish-orange and longer than the upper beak. The fin formula of halfbeak from the Seram Sea is D.13-15, P.9-12, V.5-6, A.9-12, C.7/8, and the number of scales at the lateral line is 52-58. Based on molecular identification, the halfbeak species from the Seram Sea was confirmed as Hemiramphus lutkei with a genetic distance of 0.0055 compared to H. lutkei from India, and a genetic distance of 0.0540 with H. balao.
Potential antioxidant activity of Lactobacillus fermentum KF7 from virgin coconut oil products in Padang, West Sumatra, Indonesia
Authors: SYUKUR SUMARYATI , SAFRIZAYANTI SAFRIZAYANTI , ZULAIHA SITI , SUPRIWARDI EPI , FAHMI AULIA , NURFADILAH KUNTUM KH , PURWATI ENDANG
Published: June 2022

Abstract. Syukur S, Safrizayanti, Zulaiha S, Supriwardi E, Fahmi A, Nurfadilah KK, Purwati E. 2021. Potential antioxidant activity of Lactobacillus fermentum KF7 from virgin coconut oil products in Padang, West Sumatra, Indonesia. Biodiversitas 23: 1628-1634. The isolation and characterization of five commercial Virgin Coconut Oil (VCO) products circulating in the local market of Padang city were conducted. In this study, eighteen isolates of lactic acid bacteria (LAB) were isolated and characterized microscopically, and biochemically. VCO-LAB isolates were Gram-positive bacilli and cocci, catalase-negative, and homofermentative (except VD was heterofermentative). There were three isolates of VCO-LAB (VB.3, VD and VE.4) that could produce gamma-aminobutyric acid (GABA). The highest concentration of GABA (19.5 mg/mL) was produced by isolate VB.3 after 72 hours of incubation and the addition of 7% MSG. VB.3 isolate shows the highest antioxidant activity with the addition of Monosodium glutamate (MSG), 68.13% for 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) ABTS and 88.02%. In addition, GABA-producing VCO-LAB could be grown in media containing 0.2-1.0 mM hydrogen peroxide, with the lowest optical density value of 0.82 nm. The antioxidant activity of VCO-LAB was affected by the GABA produced and by secondary metabolites such as peptides and antioxidant enzymes (NADH-oxidase, superoxide dismutase, NADH peroxidase and non-heme catalase). The highest antioxidant production was obtained in isolate VB.3 or Lactobacillus fermentum KF7.
Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression
Authors: ZULAIKA ENNY , SOLIKHAH FARIHATUS , UTOMO MUHAMMAD ANDRY PRIO , ENDAH NOOR , ASSAVALAPSAKUL WANCHAI
Published: June 2022

Abstract. Zulaika E, Solikhah F, Utomo MAP, Endah N, Assavalapsakul W. 2022. Cellulose degrading bacteria isolated from Palangkaraya, Central Kalimantan, Indonesia as peat fiber decomposer to accelerate peat soil compression. Biodiversitas 23: 1648-1654. Peat soil was established from incomplete weathered plants. It delays the process of soil compression. The main components of peat soil are fibers which made of cellulose and lignin that can be enzymatically degraded by cellulolignolytic bacteria. The objective of this study was to isolate indigenous lignocellulolytic bacteria from peat soil that perform peat fiber degradation to accelerate soil compression process. A bacteria screening test was carried out on carboxymethyl cellulose (CMC) media and bacteria identification using 16S rRNA gene markers. Peat fiber decomposition test based on biomass loss, decomposition results were visualized using scanning electron microscopy (SEM) and analyzed using Fourier Transform Infrared (FTIR). The results of screening and identification obtained 5 bacterial species, namely Pseudomonas taiwanensis U3 and Bacillus cereus D1, D3, U2, U4. After six weeks of incubation, P. taiwanensis U3 showed the highest cellulase activity than other isolates with 90% decomposition ability of peat fibers. The results of the decomposition of peat fibers with the addition of isolates visualized smaller pore sizes than control. Stretching occurs in the aromatic group of the C-O ester bond, the CºC structure of the alkyne group, and the O-H of the alcohol. All obtained bacteria isolates were still viable after six weeks of incubation with 104 CFU g-1 dry weight of fiber. The present investigation deals with isolating indigenous lignocellulolytic bacteria from peat soil that perform peat fiber degradation to accelerate the soil compression process. Our findings are also the first publication that reported P. taiwanensis cellulolytic potential.
Chemical and hedonic characteristics of smoked Katsuwonus pelamis (fufu fish) from Sorong, West Papua, Indonesia
Authors: SAYUTI MOHAMMAD , SALAMPESSY RANDI BOKHY SYULIANA , ASRIANI ASRIANI , NURBANI SITI ZACHRO , SAIDIN SAIDIN
Published: June 2022

Abstract. Sayuti M, Salampessy RBS, Asriani, Nurbani SZ, Saidin. 2022. Chemical and hedonic characteristics of smoked Katsuwonus pelamis (fufu fish) from Sorong, West Papua, Indonesia. Biodiversitas 23: 1707-1713. Fufu fish is one of the smoked fish products from eastern Indonesia, especially the North Sulawesi and Papua region, with raw materials derived from skipjack tuna or yellow-fin tuna. Samples were obtained from fish-smoking entrepreneurs in Sorong City. Proximate analysis was carried out using the association of official analytical chemist (AOAC) standard, amino acid analysis was carried out using liquid chromatography-mass spectrometry (LC-MS), fatty acid analysis was carried out using gas chromatography-mass spectrometry (GC-MS), and hedonic analysis was carried out using the SNI 2346:2015 standard with 120 panelists. The results showed that fufu fish had a water content of 64.52%, ash content of 0.82%, total fat of 1.97%, protein of 27.75%, carbohydrates of 4.37%, and crude fiber of 2.69%. The main essential amino acids of fufu fish were L-Lycine by 5.59%, L-Histidine by 3.25%, and L-Leucine by 2.61%. On the other hand, the main non-essential amino acids were L-Glutamic acid by 3.83%, L-Aspartic acid by 2.60%, and L-Arginine by 2.43%. The main fatty acids of fufu fish were C15:1 (cis-10) by 47.43%, C14:0 by 16.75%, C16:0 by 12.20%, and C18:0 by 10.97%. On the fufu fish hedonic test results, the panelists generally gave a like score. Based on these findings, it can be concluded that fufu fish had good nutritional content for the community.
Climate emergency: how the inequality crisis is dynamically linked to the sociogenesis of climate change
Authors: Harvey Mark
Published: June 2022

The failure of COP26 to secure binding commitments delivering a pathway to global warming limited to 1.5°C is attributable to a UN political process that prevents addressing the inequalities between and within nations in generating greenhouse gases. Historical divergences of national wealth and the present extreme inequalities of purchasing power (Piketty, Milanovic, Savage) manifest themselves in how the richest people in the richest nations are now the leading forcers of climate change. A second dimension of inequality, receiving less attention, concerns the inequalities between nations of environmental resources in fossil energy, agricultural land, minerals and renewable alternatives. The concept of sociogenesis of climate change analyses the combination of these two dimensions of inequality to account for the present political impasse, national and international. A dominant feature of a nation’s wealth has historically been based on the unrestricted exploitation of its own environmental resources, or those that it commands through colonisation or trade. This has resulted in the US now producing more than double the CO₂eq per capita than China, or Germany consuming four times more coal per capita than India. The COP26 impasse on coal and fossil fuels arose in part from China’s and India’s unwillingness to strand its environmental assets without alternative pathways to equivalent national wealth, while wealthier nations continue to excessively exploit theirs. A sociogenic analysis of wealth and environmental resource inequalities signals the need for a radical change in the political processes required to mitigate the climate emergency.
‘Lizzie’s story felt like home’: Narrative intimacy in The Lizzie Bennet Diaries fandom
Authors: Dabek Meredith
Published: June 2022

In 2012, The Lizzie Bennet Diaries (LBD) began to reimagine Austen’s beloved novel, Pride and Prejudice, for the twenty-first century by conveying the narrative through multiple digital media platforms. LBD centred on a series of YouTube video diaries ostensibly uploaded by Lizzie Bennet, a 20-something graduate student. The LBD narrative eventually expanded to include four complementary YouTube channels, thirteen interconnected Twitter feeds, Tumblr posts, Facebook profiles and numerous social media interactions and ‘conversations’ between the narrative’s characters and its fans. One of the key features of LBD was how the narrative actively invited fans and readers to participate and interact with the characters through its multiple media platforms, providing fans with the sense that their contributions to the narrative mattered and creating what Sarah K. Day has termed ‘narrative intimacy’. According to Day, texts with narrative intimacy ‘reflect, model, and reimagine intimate interpersonal relationships through the disclosure of information and the experience of the story as a space that the narrator invites the reader to share’. This article explores how LBD used the interactive and participatory functions of YouTube, Twitter and Tumblr to create a deeply immersive narrative experience for fans and how that immersion helped contribute to the development of LBD’s narrative intimacy through the use of Lizzie and Lydia as vulnerable narrators. Drawing on results from the author’s mixed-methods survey of LBD fans, this article highlights the lasting affective attachment fans have to the narrative and how their LBD experience reinforced their connection to Austen’s original novel.
La huella de Mario Levrero en Días laborables (2018) de Diego Otero
Authors: Castañeda Arévalo Luis Hernán
Published: June 2022

En este artículo se analiza la novela Días laborables (2018) del poeta y narrador peruano Diego Otero para trazar una relación intertextual con la poética del narrador uruguayo Mario Levrero. Mediante el análisis de las estrategias narrativas de Otero, se postula que su novela sigue mecanismos centrífugos de distracción, dilación o digresión que interrumpen el hilo lineal de la narración y la plantilla de (sub)género –la novela policiaca, el thriller–para dar cabida a las emanaciones del inconsciente. Al mismo tiempo, se valora que la ética creadora de Levrero es objeto de la admiración y la emulación de Otero, tal como el escritor limeño revela en una entrevista (aún inédita) aquí presentada. Finalmente, se concluye que el parentesco entre Otero y Levrero los sitúa a ambos fuera de los ámbitos del mercado y laacademia, y dentro de lo que Damián Tabarovsky llamó ‘literatura de izquierda’.
Vida y obra del poeta peruano José María Eguren: Análisis del poema «Marioneta misteriosa» (la ternura como centro de la poesía egureniana)
Authors: Segura Zariquiegui Ainhoa
Published: June 2022

La literatura peruana posee grandes autores que se despliegan a lo largo de las diversas épocas de su historia. Este artículo pretende mostrar la vida y la obra de uno de los poetas más grandes de Perú. Se trata de José María Eguren (1874-1942), poeta incluido en la estética modernista. La primera parte del trabajo se dedica a los aspectos personales del autor donde se conocerá el proceso de crecimiento y maduración artística y en la segunda, pasaremos a internarnos en sus versos para tratar desvelar el misterio que envuelve su poesía, analizando el poema «La marioneta misteriosa» y sacando a relucir aspectos poéticos poco estudiados por la crítica como es el concepto de la ternura egureniana.
"Epigramas" de Ernesto Cardenal: cantos de anuncio y denuncia
Authors: Rivera Vaca Alberto
Published: June 2022

Para Ernesto Cardenal la poesía es anuncio y denuncia. Esta es una poética que aparece con insistencia en los versos de desengaño amoroso de Epigramas y se extiende paralelamente a los versos de inquietud social. La poética del anuncio y la denuncia puede explicarse mediante tres aspectos esenciales de la escritura del poeta: la transformación y permanencia de ciertos factores y funciones de la comunicación y el lenguaje, lo cual explica la fugacidad de la realidad y la perpetuidad de la poesía, y el vehemente impulso por comunicar el anuncioy la denuncia en forma de clamor, canto y plegaria en Epigramas y en otros poemarios. Para indagar en estos temas recurro al análisis textual y a los conceptos de factores y funciones de la comunicación y el lenguaje.
«Yo digo poema porque no puedo pronunciar la r». La potencia-de-no en Notas para un seminario sobre Foucault de Mario Montalbetti
Authors: Alvarado Ramos Vladimir Litmam
Published: June 2022

El concepto de potencia-de-no de Giorgio Agamben propone que todo acto de creación no solamente resiste, sino que también se opone a la expresión en el sentido que detiene su movimiento hacia el acto y conserva su potencia. Por lo tanto, en este trabajo pretendo explicar a través de este concepto cómo se construye el lugar del poema en el libro Notas para un seminario sobre Foucault (2018) del poeta peruano Mario Montalbetti. Al establecer esta construcción es posible leer la negatividad del poemario como una suspensión y exposición del lenguaje mismo que se representan a través de los rasgos textuales. En consecuencia, Notas dice lo que no hace, haciendo lo que no dice. Y así, el lugar del poema está en su propia negación.
Anastasia Krutitskaya. Pliegos de villancicos conservados en ocho bibliotecas mexicanas. México-Madrid-Frankfurt: Universidad Nacional Autónoma de México- Iberoamericana-Vervuert, 2020
Authors: Stein Tadeo Pablo
Published: June 2022

Reseña de Tadeo Pablo Stein sobre Pliegos de villancicos conservados en ocho bibliotecas mexicanas. (Iberoamericana, 2020) de Anastasia Krutitskaya. 494 p. ISBN: 978-84-9192-090-8.
Medical rescuers’ occupational health during COVID-19: Contribution of coping and emotion regulation on burnout, trauma and post-traumatic growth
Authors: Fonseca Sílvia Monteiro , Cunha Sónia , Campos Rui , Faria Sara , Silva Márcio , Ramos M. Joaquina , Azevedo Guilherme , Barbosa António Ruão , Queirós Cristina
Published: June 2022

The COVID-19 pandemic places unique challenges to medical rescuers’ occupational health. Thus, it is crucial to assess its direct and indirect impacts on key psychological outcomes and adaptation strategies. This study aims to analyse the impact of this pandemic on medical rescuers’ coping and emotion regulation strategies, and their levels of work-related psychological outcomes, such as burnout, trauma and post-traumatic growth. Additionally, it aims to analyse the contribution of coping and emotion regulation strategies, employed to manage the COVID-19 pandemic, on burnout, trauma and post-traumatic growth. A sample of 111 medical rescuers answered the Brief Cope, Emotion Regulation Questionnaire, Oldenburg Burnout Inventory, Impact of Event Scale-Revised and Post-Traumatic Growth Inventory. Medical rescuers resorted moderately to coping and emotion regulation strategies, since the beginning of COVID-19. They presented moderate burnout and post-traumatic growth and low trauma. Coping presented a higher weight on burnout, trauma and post-traumatic growth, than emotion regulation. Expressive suppression and dysfunctional coping predicted burnout and trauma, and problem and emotion-focused coping predicted post-traumatic growth. Dysfunctional coping mediated and, thus, exacerbated the effect of expressive suppression on burnout and on trauma. Practitioners should pay closer attention to professionals with higher burnout and trauma. Occupational practices should focus on reducing dysfunctional coping and expressive suppression and promoting problem-focused coping.
Inclusive Teaching Program: A seven-module online course for faculty of higher education
Authors: Suarez-Grant Jeff , Haras Catherine
Published: June 2022

The Center for Effective Teaching and Learning at Cal State LA (CETL) was tasked to create a certificate program to address inclusive teaching that we could offer to faculty online at scale. The Inclusive Teaching Program recognizes college faculty as independent experts and self-directed learners who desire space and community to think about their roles as the single greatest influencers of student success. We ground our program design in Wlodkowski and Ginsberg’s (2017) framework of adult motivation to learn, while being cognizant that highly professional people (who include faculty) are among the most resistant to change (Argyris, 1991). The program takes an expansive view of, and broadly frames, the concept of inclusivity (Holladay et al., 2003; Rios & Wynn, 2016). The resulting seven-module course deeply engages instructors in the following areas: teaching identity, working with our diverse and unique population of students, leading difficult discussions, acknowledging implicit bias, and practicing faculty care and self-care. We share a novel infrastructure for creating educational development that tackles difficult topics while helping faculty make meaningful changes to their teaching.
Building Cultural Competency in Interior Design: When Good Intentions go Awry
Authors: Young Barbara
Published: June 2022

A local professional organization for interior designers issued a student design competition for a community cultural center to celebrate and teach others about a specific culture of the student’s choosing. Culture was broadly defined beyond race, nationality, religious, and social beliefs to include any group with shared interests. Students were encouraged to select a culture they might ‘admire’ and want to learn more about. The premise of the project was well intentioned, however the brief embodied outdated notions of culture and contained language which was arguably insensitive. Adding these issues to underlying problems of ‘pick-a-culture’ projects, my first inclination was to decline to participate. However, at a time when the COVID-19 pandemic exposed racial disparities in the US culture and economy, I recognized the critical importance of bringing conversations about race, privilege, stereotypes, appropriation, and advocacy into the classroom through reading, critique, and discussion. This paper raises multiple questions about the role of design, design education, and inclusion that surfaced through the project and identifies my own faults in the process. I question: who is allowed to design for ‘others’, was this an appropriate opportunity for minority students to use their own voice, and did I imbue my white guilt into the class? I will also discuss how the intention of design as problem-solving surfaced as power and privilege in conceptual development.