bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the Color Stability, Stainability, and Surface Roughness of Permanent Composite-Based Milled and 3D Printed CAD/CAM Restorative Materials after Thermocycling
Authors: Bozoğulları Hatice Nalan , Temizci Tuğba
Published: October 2023

With the developments in digital dentistry in recent years, subtractive and additive manufacturing and materials have emerged for the production of dental restoration. Novel permanent composite-based restorative materials are also among these materials. Because of their variety and increased use by clinicians, it is also important to know the properties, such as surface roughness and color stability, that are necessary for the longevity of these new materials. This study aimed to investigate the color stability, stainability, and surface roughness (Ra) of additively and subtractively manufactured permanent composite-based restorative materials and compare them with a feldspathic glass ceramic. Two different subtractively manufactured composite-based blocks (Cerasmart 270, Vita Enamic), two different additively manufactured permanent composite-based resins (Crowntec and Permanent Crown Resin), and one feldspathic glass ceramic (Vita Mark II) as a control were compared. A total of 150 specimens were prepared (10 per material for surface roughness and 20 per material for color stability and stainability). The Ra values and the color parameters were measured before and after thermocycling. The specimens of the staining test were then divided into two subgroups and stored for 7 days in distilled water (control) or coffee. The color parameters were remeasured after the storage period. The color differences (∆E00) were evaluated for each measuring range, and these values are interpreted in terms of clinical perceptibility (ΔE00 ≤ 1.30) and clinical acceptability (ΔE00 ≤ 2.25) thresholds. All data were statistically analyzed (α = 0.05). While Vita Enamic exhibited the highest mean Ra, Crowntec showed the lowest mean Ra after thermocycling. Crowntec had the highest mean ΔE00 both after thermocycling and storage in solutions. While Vita Mark II displayed the lowest mean ΔE00 after thermocycling. Cerasmart 270 showed the lowest stainability in coffee. All tested materials showed acceptable surface roughness after thermocycling that was equal to or below the plaque accumulation threshold of 0.2 µm. However, the mean ΔE00 of tested materials were lower than clinical acceptability thresholds, except for Crowntec in all measuring ranges and Vita Enamic immersed in coffee.
Reconsidering the “Popular View” (俗覧 zokuran): Tracing Vernacular Precedents in a Modern Illustrated Hagiography of Kakuban 覺鑁 (1095–1143)
Authors: Hayes Matthew
Published: October 2023

As a supplement to sermonizing, the use of images has been crucial to growing the lay Buddhist following in Japan since at least the tenth century. While it may be the case that Buddhist images, much more so than texts, have historically been better able to draw in popular audiences through their accessible means of communication, the emergence of contemporary literate audiences meant new modes of accessibility. This article explores both the textual and illustrative histories of a modern illustrated hagiography on the medieval Shingon Buddhist monk Kakuban 覺鑁 (1095–1143). By tracing earlier vernacular approaches to Kakuban’s narrative that emerged throughout the evolution of this hagiography, it becomes clear that images were merely auxiliary in their appeal to modern Japanese readers and that such an appeal had been a consideration for generations of Buddhist compilers. This example draws attention to the mutually constitutive relationship between otherwise traditionally distinct functions of text and image in Japanese Buddhist hagiography, but also common conceptual divisions between lay and monastic experiences and popular and elite reading practices.
Numerical Simulation of Constrained Flows through Porous Media Employing Glimm’s Scheme
Authors: da Gama Rogério M. Saldanha , Pedrosa Filho José Julio , da Gama Rogério Pazetto S. , da Silva Daniel Cunha , Alexandrino Carlos Henrique , Martins-Costa Maria Laura
Published: October 2023

This work uses a mixture theory approach to describe kinematically constrained flows through porous media using an adequate constitutive relation for pressure that preserves the problem hyperbolicity even when the flow becomes saturated. This feature allows using the same mathematical tool for handling unsaturated and saturated flows. The mechanical model can represent the saturated–unsaturated transition and vice-versa. The constitutive relation for pressure is a continuous and differentiable function of saturation: an increasing function with a strictly convex, increasing, and positive first derivative. This significant characteristic permits the fluid to establish a tiny controlled supersaturation of the porous matrix. The associated Riemann problem’s complete solution is addressed in detail, with explicit expressions for the Riemann invariants. Glimm’s semi-analytical scheme advances from a given instant to a subsequent one, employing the associated Riemann problem solution for each two consecutive time steps. The simulations employ a variation in Glimm’s scheme, which uses the mean of four independent sequences for each considered time, ensuring computational solutions with reliable positions of rarefaction and shock waves. The results permit verifying this significant characteristic.
Genetic Predisposition to SARS-CoV-2 Infection: Cytokine Polymorphism and Disease Transmission within Households
Authors: Saal Marius , Loeffler-Wirth Henry , Gruenewald Thomas , Doxiadis Ilias , Lehmann Claudia
Published: October 2023

We addressed the question of the influence of the molecular polymorphism of cytokines from different T helper subsets on the susceptibility to SARS-CoV-2 infection. From a cohort of 527 samples (collected from 26 May 2020 to 31 March 2022), we focused on individuals living in the same household (n = 58) with the SARS-CoV-2-infected person. We divided them into households with all individuals SARS-CoV-2 PCR positive (n = 29, households, 61 individuals), households with mixed PCR pattern (n = 24, 62) and negative households (n = 5, 15), respectively. TGF-β1 and IL-6 were the only cytokines tested with a significant difference between the cohorts. We observed a shift toward Th2 and the regulatory Th17 and Treg subset regulation for households with all members infected compared to those without infection. These data indicate that the genetically determined balance between the cytokines acting on different T helper cell subsets may play a pivotal role in transmission of and susceptibility to SARS-CoV-2 infection. Contacts infected by their index persons were more likely to highly express TGF-β1, indicating a reduced inflammatory response. Those not infected after contact had a polymorphism leading to a higher IL-6 expression. IL-6 acts in innate immunity, allergy and on the T helper cell differentiation, explaining the reduced susceptibility to SARS-CoV-2.
A Nexus between Genetic and Non-Genetic Mechanisms Guides KRAS Inhibitor Resistance in Lung Cancer
Authors: Kulkarni Prakash , Mohanty Atish , Ramisetty Sravani , Duvivier Herbert , Khan Ajaz , Shrestha Sagun , Tan Tingting , Merla Amartej , El-Hajjaoui Michelle , Malhotra Jyoti , Singhal Sharad , Salgia Ravi
Published: October 2023

Several studies in the last few years have determined that, in contrast to the prevailing dogma that drug resistance is simply due to Darwinian evolution—the selection of mutant clones in response to drug treatment—non-genetic changes can also lead to drug resistance whereby tolerant, reversible phenotypes are eventually relinquished by resistant, irreversible phenotypes. Here, using KRAS as a paradigm, we illustrate how this nexus between genetic and non-genetic mechanisms enables cancer cells to evade the harmful effects of drug treatment. We discuss how the conformational dynamics of the KRAS molecule, that includes intrinsically disordered regions, is influenced by the binding of the targeted therapies contributing to conformational noise and how this noise impacts the interaction of KRAS with partner proteins to rewire the protein interaction network. Thus, in response to drug treatment, reversible drug-tolerant phenotypes emerge via non-genetic mechanisms that eventually enable the emergence of irreversible resistant clones via genetic mutations. Furthermore, we also discuss the recent data demonstrating how combination therapy can help alleviate KRAS drug resistance in lung cancer, and how new treatment strategies based on evolutionary principles may help minimize or even preclude the emergence of drug resistance.
The Effects of Deregulated Ribosomal Biogenesis in Cancer
Authors: Lu Yiwei , Wang Shizhuo , Jiao Yisheng
Published: October 2023

Ribosomes are macromolecular ribonucleoprotein complexes assembled from RNA and proteins. Functional ribosomes arise from the nucleolus, require ribosomal RNA processing and the coordinated assembly of ribosomal proteins (RPs), and are frequently hyperactivated to support the requirement for protein synthesis during the self-biosynthetic and metabolic activities of cancer cells. Studies have provided relevant information on targeted anticancer molecules involved in ribosome biogenesis (RiBi), as increased RiBi is characteristic of many types of cancer. The association between unlimited cell proliferation and alterations in specific steps of RiBi has been highlighted as a possible critical driver of tumorigenesis and metastasis. Thus, alterations in numerous regulators and actors involved in RiBi, particularly in cancer, significantly affect the rate and quality of protein synthesis and, ultimately, the transcriptome to generate the associated proteome. Alterations in RiBi in cancer cells activate nucleolar stress response-related pathways that play important roles in cancer-targeted interventions and immunotherapies. In this review, we focus on the association between alterations in RiBi and cancer. Emphasis is placed on RiBi deregulation and its secondary consequences, including changes in protein synthesis, loss of RPs, adaptive transcription and translation, nucleolar stress regulation, metabolic changes, and the impaired ribosome biogenesis checkpoint.
A Novel Molecularly Imprinted Sensor Based on CuO Nanoparticles with Peroxidase-like Activity for the Selective Determination of Astragaloside-IV
Authors: Chen Guo-Ying , Chen Ling-Xiao , Gao Jin , Chen Chengyu , Guan Jianli , Cao Zhiming , Hu Yuanjia , Yang Feng-Qing
Published: October 2023

In this work, dopamine (DA) was polymerized on the surface of CuO nanoparticles (CuO NPs) to form a molecularly imprinted polymer (MIP@PDA/CuO NPs) for the colorimetric detection of astragaloside-IV (AS-IV). The synthesis process of MIP is simple and easy to operate, without adding other monomers or initiators. CuO NPs has high peroxidase (POD)-like activity that can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate oxidized TMB (OxTMB) in the presence of H2O2, having a maximum ultraviolet-visible (UV-Vis) absorption peak at 652 nm. The AS-IV can specifically bind to the surface imprinted cavities and prevent the entry of TMB and H2O2, which will lead to the inhibition of the catalytic reaction. Therefore, a new approach based on the POD-like activity of MIP@PDA/CuO NPs for AS-IV detection was developed with a linear range from 0.000341 to 1.024 mg/mL. The LOD and LOQ are 0.000991 and 0.000341 mg/mL, respectively. The developed method can accurately determine AS-IV in Huangqi Granules and different batches of Ganweikang Tablets, which are similar to the results measured by HPLC-ELSD and meet the requirements of Chinese Pharmacopoeia (2020 edition) for the amount of AS-IV in Huangqi Granules. The combination of MIP with CuO NPs not only endows the detection of AS-IV with high selectivity and reliability, but also expands the application of nanozymes in the detection of small-molecule compounds that have weak UV absorption, and do not have reducibility or oxidation properties.
Attention Deficit Hyperactivity Disorder Misdiagnosis: Why Medical Evaluation Should Be a Part of ADHD Assessment
Authors: Sadek Joseph
Published: October 2023

Introduction: Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder that interferes with multiple aspects of daily functioning and is associated with impairments in several domains. It may affect academic, educational, vocational, social, emotional, interpersonal, and health domains, and worsen risks to health outcomes. Objective: To identify and discuss medical conditions that commonly present with symptoms resembling ADHD. Method: This review is selective and not systematic. It is conducted through a focused literature search through PubMed, Google Scholar, and EMBASE. Search term included “ADHD misdiagnosis”, “medical conditions with ADHD like symptoms”, “ADHD AND medical problems”. Exclusion: giftedness, high IQ, and any article that does not list medical conditions. The limits applied were the following: the work must have been published in the past 20 years, be on humans, and be in the English language. Results: There are several medical conditions that can be misdiagnosed as ADHD and may show a similar presentation to ADHD, particularly with inattentive symptoms. Examples include, but are not limited to, absence seizure disorder, diabetes, thyroid dysfunction, sleep deprivation, post-concussion states, inflammatory bowel disease, iron deficiency states and anemia, and disordered breathing. Conclusions: Our review suggests that a thorough medical evaluation should be conducted prior to the diagnosis of ADHD. Allied health professionals and psychologists who diagnose ADHD should seek medical clearance from a physician prior to making the ADHD diagnosis in order to reduce misdiagnosis rates and improve patient outcomes. ADHD diagnosis should follow guidelines and be carried out under a systematic standardized approach. A full medical evaluation should be conducted to assess for medical conditions that may look like ADHD or be associated with ADHD.
Objective Assessment of Perilymphatic Fistula in Cases of Postoperative Vertigo after Cochlear Implantation by Cochlin Tomoprotein (CTP)
Authors: Todt Ingo , Ikezono Tetsuo
Published: October 2023

Objective: Vertigo is a quite frequent complication after cochlear implantation. Perilymphatic fistula (PLF) is assumed to be one cause of this problem. Cochlin tomoprotein (CTP) is a newly introduced marker for PLF. The present aim was to evaluate the rate of positive CTP testing in cases of newly occurring vertigo after cochlear implantation. Materials and Methods: Twelve patients with vertigo after cochlear implantation and a revisional electrode-sealing procedure underwent intraoperative rinsing of their middle ear. The sample was evaluated for CTP with monoclonal antibody testing. Sixteen controls from six CI patients were taken. Results: 4 out of 12 (33%) cases showed positive CTP testing, indicating that a PLF could be evaluated. In all of the positive CTP cases, surgery decreased the vertigo symptoms. A relation between the subjective visual assessment of a fistula and a positive CTP value was not observed. Controls confirmed the value of the testing. Discussion: CTP detection objectively shows that PLF can occur in patients with vertigo after CI.
Neuropsychological Functioning in Bilateral versus Unilateral Temporal Lobe Epilepsy
Authors: Baggio Martina , Danieli Alberto , Crescentini Cristiano , Duma Gian Marco , Da Rold Martina , Baldini Sara , Pascoli Eric , Antoniazzi Lisa , Vestri Alec , Fabbro Franco , Bonanni Paolo
Published: October 2023

Although relatively specific anatomo-electro-clinical features of temporal lobe epilepsy (TLE) with bilateral ictal involvement (bitemporal epilepsy—BTLE) have been described, differentiating between BTLE and unilateral TLE (UTLE) remains challenging. Surgery is often the treatment of choice for drug-resistant UTLE, whereas its use is more controversial in BTLE. It is currently unclear whether neuropsychological assessment can contribute to the differential diagnosis. We retrospectively reviewed the neuropsychological evaluation of 46 consecutive patients with refractory TLE. Eighteen patients were diagnosed with BTLE on the basis of ictal electro-clinical data, in particular a video EEG recording of at least one seizure simultaneously involving the two temporal lobes without the possibility of lateralizing its onset or at least two different seizures independently arising from the two temporal lobes. Twenty-eight patients were classified as UTLE. Presurgery evaluation data were used in this study. Compared with UTLE, BTLE was associated with a lower intelligence quotient (IQ) and more severe impairment in long-term memory, the latter remaining significant even after controlling for IQ. No significant differences were found between right and left UTLE. In conclusion, BTLE and UTLE are associated with relatively distinct neuropsychological profiles, further supporting their classification as different disorders within the TLE spectrum.
Neuromodulation Techniques in Children with Super-Refractory Status Epilepticus
Authors: Stavropoulos Ioannis , Pak Ho Lim , Alarcon Gonzalo , Valentin Antonio
Published: October 2023

Status epilepticus (SE) is a life-threatening condition and medical emergency which can have lifelong consequences, including neuronal death and alteration of neuronal networks, resulting in long-term neurologic and cognitive deficits in children. When standard pharmacological treatment for SE is not successful in controlling seizures, the condition evolves to refractory SE (rSE) and finally to super-refractory SE (srSE) if it exceeds 24 h despite using anaesthetics. In this systematic review, we present literature data on the potential uses of clinical neuromodulation techniques for the management of srSE in children, including electroconvulsive therapy, vagus nerve stimulation, and deep brain stimulation. The evaluation of these techniques is limited by the small number of published paediatric cases (n = 25, one with two techniques) in peer-reviewed articles (n = 18). Although neuromodulation strategies have not been tested through randomised, prospective controlled clinical trials, this review presents the existing data and the potential benefits of neuromodulation therapy, suggesting that these techniques, when available, could be considered at earlier stages within the course of srSE intending to prevent long-term neurologic complications. Clinical trials aiming to establish whether early intervention can prevent long-term sequelae are necessary in order to establish the potential clinical value of neuromodulation techniques for the treatment of srSE in children.
Comparison of the 10-, 14- and 20-Item CES-D Scores as Predictors of Cognitive Decline
Authors: Jauregi-Zinkunegi Ainara , Langhough Rebecca , Johnson Sterling C. , Mueller Kimberly D. , Bruno Davide
Published: October 2023

The association between depressive symptomatology and cognitive decline has been examined using the Centre for Epidemiologic Studies-Depression Scale (CES-D); however, concerns have been raised about this self-report measure. Here, we examined how the CES-D total score from the 14- and 10-item versions compared to the 20-item version in predicting progression to cognitive decline from a cognitively unimpaired baseline. Data from 1054 participants were analysed using ordinal logistic regression, alongside moderator and receiver-operating characteristics curve analyses. All baseline total scores significantly predicted progression to cognitive decline. The 14-item version was better than the 20-item version in predicting consensus diagnosis, as shown by their AICs, while also showing the highest accuracy when discriminating between participants by diagnosis at last visit. We did not find sex to moderate the relationship between CES-D score and cognitive decline. Current findings suggest the 10- and 14-item versions of the CES-D are comparable to the 20-item version, and that the 14-item version may be better at predicting longitudinal consensus diagnosis compared to the 20-item version.
Negative Life Events on Depression of Vocational Undergraduates in the Partial Least Squares Structural Equation Modeling Approach Perspective: A Mediated Moderation Model
Authors: Zhang Sensen , Ding Fengqin , Sun Yishu , Jing Zhi , Li Ning
Published: October 2023

Background: Following China’s strategy of developing applied and compound social talents, vocational undergraduates are surging rapidly, and it is essential to understand the causes of their depression to effectively prevent and intervene in schools. Objective: We aimed to investigate the relationship between negative life events (NLEs) and depression among vocational undergraduates in China, along with the mediating role of loneliness and the moderating role of socioeconomic status (SES). Methods: A convenience sample survey was conducted at a vocational education university (N = 1487), and analyzed using partial least squares structural equation modeling. Results: Findings showed that NLEs directly predicted depression (β = 0.399, 95% CI [0.339, 0.452], p < 0.001) among vocational undergraduates. Furthermore, this relationship was partially mediated by loneliness (β = 0.182, 95% CI [0.145, 221], p < 0.001); SES moderated the link between NLEs and depression (β = 0.051, 95% CI [0.004, 092], p < 0.05), but not between NLEs and loneliness (p > 0.05). Conclusions: The current study highlights the impact of NLEs on depression among vocational undergraduates, indicating the importance of addressing NLEs and consequent feelings of loneliness to promote mental health. In addition, the moderating role of SES underscores the necessity of targeted interventions to mitigate the impact of NLEs on depression. The present study contributes to our understanding of the unique characteristics of depression in vocational undergraduates and has practical implications for psychological support services. Moreover, it probably has broader implications for addressing mental health challenges in global education settings for vocational undergraduates.
Thermal Performance Assessment of Burkina Faso’s Housing Typologies
Authors: Aguilar-Sanchez Maria , Almodovar-Melendo Jose-Manuel , Cabeza-Lainez Joseph
Published: October 2023

Burkina Faso is a sub-Saharan African country suffering from a high poverty rate. It has quadruplicated its population over the last twenty years, that is, from 4.5 to 18 million inhabitants. Such demographic changes, together with an intense migration process from rural areas to large cities, among other issues, are contributing to a rapid process of socio-cultural transformation. In this context, changes in both traditional and contemporary housing are analyzed for this article. To this aim, a thorough thermo-hygrometric evaluation is carried out by processing significant data from on-site measurements to shed new light on the adaptation of the said dwellings to the environmental and local cultural values. Preliminary results show that the introduction of land ownership linked to climate change and the increasing scarcity of natural resources are forcing native ethnic groups to modify their traditional ways of life, even threatening their subsistence. Consequently, we can prove that the new building typologies are not suitable in the least for the climatic conditions nor the rich cultural heritage of Burkina Faso. This fact negatively affects the possibility of revitalizing vernacular and environmentally oriented solutions for the contemporary world and the necessary advance towards a more sustainable architecture. With this research, we intent to start a reversal of such a deterioration process.
Implementation of Data from Wind Tunnel Tests in the Design of a Tall Building with an Elliptic Ground Plan
Authors: Hubová Oľga , Macák Marek , Franek Michal , Ivánková Oľga , Konečná Lenka Bujdáková
Published: October 2023

The design of a 69 m tall multipurpose building was investigated in this paper. The shape of the structure above the ground was an elliptical cylinder. Under the ground, the building was extended into a cuboid shape (for car parking). External wind pressure coefficients were determined using three methods: wind tunnel tests, CFD, and “the simplification of the shape” (using information defined in building standards). From the obtained results, it was evident that the simplification did not provide results with sufficient accuracy. The external wind pressure coefficients presented in this paper should be used for the design of a similar structure. The shape of the elliptical cylinder is very sensitive to applied wind. Positive pressures only occur on a small area of the windward side. The rest of the windward side is loaded with negative pressures. Therefore, torsional effects can occur, and these can be dangerous for the structure. The leeward side is completely loaded with negative pressures. In our case, this information was necessary for a follow-on static and dynamic analysis of the building. Various subsoil stiffness coefficients were considered. The calculated horizontal displacement was compared with the limit value. A measured wind direction of 20° caused the maximum obtained torsional moment, and a wind direction of 90° induced the maximum obtained force. The commercial program Ansys Fluent 2022 was used for the CFD simulation. The SCIA ENGINEER 21 program was used for follow-on analysis. This paper presents brief information on the selected turbulence model and details the settings used for the CFD simulation. Also, a description of the wind tunnel laboratory utilized in this study is provided, along with a description of the measuring devices used and the methodologies of the tests carried out. The main purpose of this paper is to show how important it is to consider the wind load for the static analysis of a structure like this.
A Phase II Study of S-1 plus Oxaliplatin for Patients with Recurrent Non-Squamous Cell Carcinoma of the Uterine Cervix (Tohoku Gynecologic Cancer Unit: TGCU206 Study)
Authors: Nagasawa Takayuki , Shoji Tadahiro , Takatori Eriko , Kaido Yoshitaka , Kagabu Masahiro , Shimizu Dai , Shigeto Tatsuhiko , Baba Tsukasa , Sugiyama Toru , Yokoyama Yoshihito
Published: October 2023

Recurrent non-squamous cell carcinoma (non-SCC) of the uterine cervix is resistant to treatment and has a poor prognosis. The efficacy and safety of S-1/oxaliplatin (SOX) therapy in patients with recurrent non-SCC was examined in a phase II study. Fifteen patients were enrolled between August 2013 and March 2023. S-1 was administered orally at a daily dose of 80–120 mg for 14 days, and oxaliplatin was administered intravenously at a dose of 100 mg/m2 on day 1. Each treatment cycle lasted 21 days. The anti-tumor effects, adverse events, progression-free survival (PFS), and overall survival (OS) were investigated. The median patient age was 54 (41–74) years. The anti-tumor effect was rated as a partial response in five patients, stable disease in four, and progressive disease in 6. The overall response rate was 33% and the disease control rate was 60%. Regarding hematologic toxicities of grade 3 or more severity, leukopenia, neutropenia, anemia, and thrombocytopenia occurred in 26.6–40.0%. None of the patients discontinued the treatment because of adverse events. The median PFS and OS were 6 months (95% confidence interval [CI]: 2–11 months) and 22 months (95% CI: 11–23 months), respectively. No treatment-related deaths occurred. These results suggest that SOX therapy is useful for the treatment of recurrent non-SCC with promising anti-tumor effects and minimal adverse events.
Krüppel-like Factor 10 as a Prognostic and Predictive Biomarker of Radiotherapy in Pancreatic Adenocarcinoma
Authors: Tsai Yi-Chih , Hsin Min-Chieh , Liu Rui-Jun , Li Ting-Wei , Ch’ang Hui-Ju
Published: October 2023

The prognosis of pancreatic adenocarcinoma (PDAC) remains poor, with a 5-year survival rate of 12%. Although radiotherapy is effective for the locoregional control of PDAC, it does not have survival benefits compared with systemic chemotherapy. Most patients with localized PDAC develop distant metastasis shortly after diagnosis. Upfront chemotherapy has been suggested so that patients with localized PDAC with early distant metastasis do not have to undergo radical local therapy. Several potential tissue markers have been identified for selecting patients who may benefit from local radiotherapy, thereby prolonging their survival. This review summarizes these biomarkers including SMAD4, which is significantly associated with PDAC failure patterns and survival. In particular, Krüppel-like factor 10 (KLF10) is an early response transcription factor of transforming growth factor (TGF)-β. Unlike TGF-β in advanced cancers, KLF10 loss in two-thirds of patients with PDAC was associated with rapid distant metastasis and radioresistance; thus, KLF10 can serve as a predictive and therapeutic marker for PDAC. For patients with resectable PDAC, a combination of KLF10 and SMAD4 expression in tumor tissues may help select those who may benefit the most from additional radiotherapy. Future trials should consider upfront systemic therapy or include molecular biomarker-enriched patients without early distant metastasis.
The Mechanism of Oxymatrine Targeting miR-27a-3p/PPAR-γ Signaling Pathway through m6A Modification to Regulate the Influence on Hemangioma Stem Cells on Propranolol Resistance
Authors: Dai Yuxin , Qiu Mingke , Zhang Shenglai , Peng Jingyu , Hou Xin , Liu Jie , Li Feifei , Ou Jingmin
Published: October 2023

Objective: The proliferation and migration of hemangioma stem cells (HemSCs) induced apoptosis and adipose differentiation as well as increased the sensitivity of HemSCs to propranolol (PPNL). MiR-27a-3p negatively controlled the peroxisome-proliferator-activated receptor γ (PPAR-γ) level, counteracting the effect of PPAR-γ on HemSC progression and PPNL resistance. OMT accelerated HemSC progression and adipocyte differentiation via modulating the miR-27a-3p/PPAR-γ axis, inhibiting HemSC resistance to PPNL. In tumor-forming experiments, OMT exhibited a dose-dependent inhibitory effect on the volume of IH PPNL-resistant tumors, which was partially dependent on the regulation of m6A methylation transfer enzyme METTL3 and the miR-27a-3p/PPAR-γ axis, thereby inducing apoptosis. Conclusions: We conclude that OMT regulates IH and influences PPNL resistance via targeting the miR-27a-3p/PPAR-γ signaling pathway through m6A modification.
Only 32.3% of Breast Cancer Families with Pathogenic Variants in Cancer Genes Utilized Cascade Genetic Testing
Authors: Agiannitopoulos Konstantinos , Potska Kevisa , Katseli Anastasia , Ntogka Christina , Tsaousis Georgios N. , Pepe Georgia , Bouzarelou Dimitra , Tsoulos Nikolaos , Papathanasiou Athanasios , Ziogas Dimitrios , Venizelos Vassileios , Markopoulos Christos , Iosifidou Rodoniki , Karageorgopoulou Sofia , Giassas Stylianos , Natsiopoulos Ioannis , Papazisis Konstantinos , Vasilaki-Antonatou Maria , Psyrri Amanta , Koumarianou Anna , Matthaios Dimitrios , Zairi Eleni , Blidaru Alexandru , Banu Eugeniu , Jinga Dan Corneliu , Laçin Şahin , Özdoğan Mustafa , Papadopoulou Eirini , Nasioulas George
Published: October 2023

Background: Hereditary cancer predisposition syndromes are responsible for approximately 5–10% of all diagnosed cancer cases. In order to identify individuals at risk in a cost-efficient manner, family members of individuals carrying pathogenic alterations are tested only for the specific variant that was identified in their carrier relative. The purpose of this study was to investigate the clinical use and implementation of cascade family testing (CFT) in families of breast cancer patients with pathogenic/likely pathogenic variants (PVs/LPVs) in cancer-related predisposition genes. Methods: Germline sequencing was carried out with NGS technology using a 52-gene panel, and cascade testing was performed by Sanger sequencing or MLPA. Results: In a cohort of 1785 breast cancer patients (families), 20.3% were found to have PVs/LPVs. Specifically, 52.2%, 25.1%, and 22.7% of patients had positive findings in high-, intermediate-, and low-penetrance breast cancer susceptibility genes, respectively. Although CFT was recommended to all families, only 117 families (32.3%) agreed to proceed with genetic testing. Among the first-degree relatives who underwent CFT, 70.3% were female, and 108 of 121 (89.3%) were cancer free. Additionally, 42.7%, 36.7%, and 20.6% were offspring, siblings, and parents of the subject, respectively. Our data suggest that CFT was mostly undertaken (104/117, 88.8%) in families with positive findings in high-risk genes. Conclusions: Cascade family testing can be a powerful tool for primary cancer prevention by identifying at-risk family members. It is of utmost importance to implement genetic counseling approaches leading to increased awareness and communication of genetic testing results.
Immunotherapy Plus Locoregional Therapy Leading to Curative-Intent Hepatectomy in HCC: Proof of Concept Producing Durable Survival Benefits Detectable with Liquid Biopsy
Authors: Raj Roma , Wehrle Chase J. , Aykun Nihal , Stitzel Henry , Ma Wen Wee , Krishnamurthi Smitha , Estfan Bassam , Kamath Suneel , Kwon David C. H. , Aucejo Federico
Published: October 2023

Background: Immunotherapy has emerged as an improved systemic treatment for select patients with advanced unresectable HCC. Objective response is reported in 30% of patients, yet complete response (pCR) allowing for curative-intent resection is rare. Locoregional therapies (LRTs) seem to show synergistic effects with immunotherapy, though this effect has not been scientifically reported. We report a cohort of patients showing pCR to immunotherapy + LRT as a proof of concept for the proposed treatment approach for locally unresectable HCC. Methods: Patients with unresectable HCC treated with immunotherapy as an intended destination therapy from 2016 to 2023 were included. The electronic health record was queried for oncologic information, locoregional therapies, surgical interventions, and long-term outcomes. Circulating tumor DNA (ctDNA) testing was obtained using Guardant360, and tumor mutational burden (TMB) was defined as the number of somatic mutations per megabase. Results: Ninety-six patients with advanced HCC received immunotherapy + LRT as a destination therapy. In total, 11 of 96 patients showed a complete response according to mRECIST criteria. Four of these (36.4%) ultimately underwent curative-intent resection. The median follow-up was 24.9 (IQR 15.6–38.3) months. Overall survival rates in those with complete response at 1, 3, and 5 years were 100%, 91%, and 81.8%, respectively, which were significantly improved compared to those of the cohort not achieving pCR (p < 0.001). All four patients undergoing immunotherapy + LRT followed by curative-intent hepatectomy have no evidence of disease (NED). Of those undergoing surgery, ctDNA was cleared in 75% (n = 3), providing an additional objective measurement of complete response. All four patients were TMB+ before beginning this treatment course, with three being TMB-, indicating stable and complete disease response. Conclusions: Immunotherapy + locoregional therapy can help downstage a significant proportion of patients with initially unresectable HCC, allowing for curative-intent surgery. The survival benefit associated with complete response seems durable up to 3 years after achieving this response. ctDNA measurement was converted from positive to negative in this cohort, providing additional indication of response.
Catalytic Co-Pyrolysis of Mesua ferrea L. De-Oiled Cake and Garlic Husk in the Presence of Red-Mud-Based Catalysts
Authors: Kumar Abhishek , Komandur Janaki , Chaudhary Vasu , Mohanty Kaustubha
Published: October 2023

Utilizing lignocellulosic biomass as a renewable energy source for the production of sustainable fuel is of paramount importance. This study focuses on the catalytic co-pyrolysis of Mesua ferrea L. de-oiled cake (MDC) and Garlic husk (GH) as potential feedstocks for bio-fuel production. The pyrolysis experiments were conducted using a semi-batch reactor under inert conditions at temperatures of 500, 550, and 600 °C, with a heating rate of 10 °C min−1, a particle size below 1 mm, and an inert gas flow rate of 80 mL min−1. The findings reveal that temperature significantly influences the yield of pyrolytic products. However, GC-MS analysis detected higher oxygenated compounds in the bio-oil, negatively impacting its heating value. To improve fuel quality, co-pyrolysis with and without a catalyst for a feedstock ratio of 1:1 w/w was performed. Red mud, an alkaline waste mainly composed of Fe2O3, Al2O3, and SiO2, is a hazardous environmental concern from aluminum production and is used as a catalyst. The red-mud catalysts reduced oxygen concentration and increased carbon content, acidity, and heating value in the pyrolytic oil. GC-MS analysis of the bio-oil confirmed that using catalysts combined with MDC and GH significantly decreased the concentration of acidic and aromatic compounds, thereby improving the pyrolytic oil’s higher heating value (HHV).
The FINDING-Food Intervention: A Mixed-Methods Feasibility Study Addressing Food Insecurity
Authors: Royer Michael F. , Wharton Christopher
Published: October 2023

Food insecurity is an economic and social condition involving limited or uncertain access to food. The problem of food insecurity in communities is influenced by economic conditions, food deserts, and barriers to accessing healthy food. Individuals experiencing food insecurity often endure concurrent problems of financial instability, hunger, and poor mental and physical health. Public and non-profit services in the U.S., such as the federally supported Supplemental Nutrition Assistance Program (SNAP) and community food banks, provide food-related assistance to individuals who are at a high risk of experiencing food insecurity. Unfortunately, many individuals who qualify for these services still experience food insecurity due to barriers preventing them from accessing food. Effective approaches for removing barriers that prevent individuals from accessing food are needed to mitigate the increased risk of chronic disease among vulnerable populations. This study tested a novel food insecurity intervention called FINDING-Food, which stands for Frequent Informational Nudges Directing Individuals to Needed Goods. Informational nudges were used to promote food security through the removal of information barriers to accessing food. The intervention used in this mixed-methods feasibility study consisted of informational nudges in the form of weekly text messages that were sent to food pantry clients experiencing food insecurity (n = 24). The study aims were to test the efficacy and acceptability of the intervention by examining whether the informational nudges could enhance food pantry utilization, increase SNAP registration, and promote food security. Quantitative study results showed a lower prevalence of food insecurity in the intervention group than the control group at the end of the study. Qualitative findings revealed how intervention group participants who completed the study (n = 9) found the text messages to be helpful and informative. These study findings can enhance future food insecurity interventions aiming to eliminate barriers that prevent individuals who are food insecure from accessing healthy food.
Challenges in Pediatric Endocrinology
Authors: Laron Zvi
Published: October 2023

As the Section Editor-in-Chief, it is my pleasure to introduce the new section of Children devoted to pediatric endocrinology [...]
Impact of Swirling Flow on the Overall Cooling Effectiveness and TBCs Insulation Characteristics of Turbine Vane
Authors: Shi Li , Wu Peng , Huang Hanze , Wang Changce , Tan Xiao , Shen Yinuo , Song Jiasheng
Published: October 2023

Modern gas turbines find extensive applications in aero engines, power generation, marine, and various other industries. Most numerical studies concentrate on turbine aero-thermal performance under different external conditions with vane or coupon; there are a few published results on combustor-turbine interactions. This study reveals the cooling performance of the first stage with or without coatings to provide a reference for high-performance combustors and turbine-integrated design. The results of the study show that (1) The velocity and temperature distribution inside the combustion chamber are obviously affected by the swirling flow. A central recirculation zone is formed near the central axis, and two external recirculation zones are formed between the inlet section of the combustion chamber and the fluid reattachment point. (2) Inside the combustion chamber, the flame temperature in the central recirculation zone is relatively high, and the range of the high-temperature zone expands with the increase of axial distance. Increasing the swirl number decreases the peak temperature level in the combustion chamber. (3) Under the influence of swirl number, the greater the swirl number, the greater the cooling effectiveness of most areas on the vane surface. (4) In regions where there is a decrease in local heat flux, positive values are evident. This suggests that the application of a coating in these areas results in a reduction of heat transfer from the vane to the mainstream. (5) When comparing the coated vane to the uncoated vane, the cooling effectiveness across the entire surface is notably enhanced, with a particularly significant improvement observed on the vane’s suction side. With the increase of Cax, the difference in cooling effectiveness increment under different swirl numbers also increases.
Investigation of Supercapacitor Electrodes Based on MIL-101(Fe) Metal-Organic Framework: Evaluating Electrochemical Performance through Hydrothermal and Microwave-Assisted Synthesis
Authors: Akkinepally Bhargav , Kumar Gara Dheeraj , Reddy I. Neelakanta , Rao H. Jeevan , Nagajyothi Patnamsetty Chidanandha , Alothman Asma A. , Alqahtani Khadraa N. , Hassan Ahmed M. , Javed Muhammad Sufyan , Shim Jaesool
Published: October 2023

Supercapacitors have garnered substantial interest owing to their capacity to deliver power effectively for short-term applications. However, current supercapacitors suffer from limited stability and low-capacity storage. Metal-organic frameworks (MOFs) have emerged as a promising solution due to their high surface area and abundant active redox sites. MOF-based electrodes combined with aqueous based electrolytes have shown potential to enhance supercapacitor performance. While there is limited literature on MIL-101(Fe) MOF-based electrodes, a comparative study was conducted to investigate the supercapacitor performance of MIL-101(Fe) electrodes synthesized using hydrothermal and microwave-assisted processes. Processing parameters, such as the method used, alter the microstructure, morphology, and uniformity of supramolecular chemistry, impacting electrochemical characteristics. This study aimed to determine the active redox reactions, chemical stability, surface area, adsorption characteristics, and electrochemical characteristics of the electrodes. The electrodes from hydrothermal synthesis [MF(ht)] exhibited excellent electrochemical activity in comparison to the microwave-assisted [MF(m)] electrodes in the three-electrode configuration. At a high current density of 7 A/g, the MF(ht) electrode displayed a remarkable specific capacitance of 775.6 F/g and a good cyclic stability (82% @ 10 A/g) after 5000 galvanostatic charge–discharge cycles. At a current density of 1 A/g, the two-electrode configuration of MF(ht) yielded a high energy density of 74.7 Wh/kg at a power density of 2160 W/kg and a decent cyclic stability after 5000 cycles. The results suggest that the MF(ht) electrodes possess remarkable electrochemical properties that make them a promising candidate for advanced applications in energy storage.
Excellent Thermoelectric Performance in KBaTh (Th = Sb, Bi) Based Half-Heusler Compounds and Design of Actuator for Efficient and Sustainable Energy Harvesting Applications
Authors: Behera Debidatta , Akila Boumaza , Amraoui Rabie , Kadri Salim , Mukherjee Sanat , Salah Mostafa , Saeed Ahmed
Published: October 2023

To examine the structural, optoelectronic, thermodynamic, and thermoelectric properties of KBaTh (Th = Sb, Bi) half-Heuslers, we used the full potential, linearized augmented plane wave (FP_LAPW) approach as in the Wien2K simulator. Generalized gradient approximation (GGA), technique, was used for the structural optimization. Mechanical stability and ductility were inherent characteristics of the studied KBaTh (Th = Sb, Bi). Having band gaps of 1.31 eV and 1.20 eV for the KBaTh (Th = Sb, Bi) compounds, they have a semiconducting character. The KBaTh (Th = Sb, Bi) compounds are suggested for use in optoelectronic devices based on studies of their optical characteristics. Thermoelectric properties were investigated using the Boltzmann transport provided by the BoltzTraP software. Since the acquired figures of merit (ZT) values for the KBaTh (Th = Sb, Bi) compounds are all almost equal to one at room temperature, this demonstrates that these substances can be used in thermoelectric devices. Additionally, we used the Slack method to determine the lattice thermal conductivity of KBaTh (Th = Sb, Bi). Our research shows that the half-Heusler compounds under investigation increase actuator response time and hence can be considered as good materials for actuators.
Study on Creep Properties of Al-Zn-Mg-Cu Alloys
Authors: Zhang Wen , Su Yunhai
Published: October 2023

This article conducts high-temperature creep tests on an Al-6.5Zn-2.3Mg-2.5Cu-0.1Zr-0.2Sc alloy in a solid solution + aging state at 200 °C and 150–180 MPa. Characterization of the microstructure of the specimen after creep test fracture was performed using SEM and TEM. The results indicate that the steady-state creep rate range of the alloy was 10−9 to 10−8 s−1, which was positively correlated with applied stress, while the creep life was negatively correlated with applied stress. Through failure analysis, it was found that the main deformation mechanism of the alloy was the dislocation climbing mechanism. The fracture mode of the alloy was ductile fracture.