bluuebunny/crossref_metadata_2025 huggingface dataset:
Chitosan Oligosaccharide Attenuates Nonalcoholic Fatty Liver Disease Induced by High Fat Diet through Reducing Lipid Accumulation, Inflammation and Oxidative Stress in C57BL/6 Mice
Authors: Tao Wenjing , Sun Wanjing , Liu Lujie , Wang Geng , Xiao Zhiping , Pei Xun , Wang Minqi
Published: November 2019

Nonalcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease closely associated with metabolic syndrome, but there are no validated pharmacological therapies. The aim of this study was to investigate the effect of chitosan oligosaccharide (COS) on NAFLD. Mice were fed either a control diet or a high-fat diet (HFD) with or without COS (200 or 400 mg/kg body weight (BW)) by oral gavage for seven weeks. Administration with COS significantly lowered serum lipid levels in the HFD-fed mice. The hepatic lipid accumulation was significantly decreased by COS, which was attributed to decreased expressions of lipogenic genes and increased expressions of fatty β-oxidation-related genes. Moreover, pro-inflammatory cytokines, neutrophils infiltration, and macrophage polarization were decreased by COS in the liver. Furthermore, COS ameliorated hepatic oxidative stress by activating the nuclear factor E2-related factor 2 (Nrf2) pathway and upregulating gene expressions of antioxidant enzymes. These beneficial effects were mediated by the activation of the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Therefore, COS might be a potent dietary supplement to ameliorate NAFLD.
Piltunines A–F from the Marine-Derived Fungus Penicillium piltunense KMM 4668
Authors: Afiyatullov Shamil Sh. , Zhuravleva Olesya I. , Antonov Alexandr S. , Leshchenko Elena V. , Pivkin Mikhail V. , Khudyakova Yuliya V. , Denisenko Vladimir A. , Pislyagin Evgeny A. , Kim Natalya Yu. , Berdyshev Dmitrii V. , Amsberg Gunhild von , Dyshlovoy Sergey A.
Published: November 2019

Six new carotane sesquiterpenoids piltunines A–F (1–6) together with known compounds (7–9) were isolated from the marine-derived fungus Penicillium piltunense KMM 4668. Their structures were established using spectroscopic methods. The absolute configurations of 1–7 were determined based on circular dichroism (CD) and nuclear Overhauser spectroscopy (NOESY) data as well as biogenetic considerations. The cytotoxic activity of some of the isolated compounds and their effects on regulation of reactive oxygen species (ROS) and nitric oxide (NO) production in lipopolysaccharide-stimulated macrophages were examined.
Multi-Antibiotic Resistance and Factors Affecting Carriage of Extended Spectrum β-Lactamase-Producing Enterobacteriaceae in Pediatric Population of Enugu Metropolis, Nigeria
Authors: Oli Angus N. , Ogbuagu Vitalis I. , Ejikeugwu Chika P. , Iroha Ifeanyichukwu R. , Ugwu Malachy C. , Ofomata Chijioke M. , Okeke Kenneth N. , Emechebe George O. , Okoro Jude C. , Okani Chukwudi O. , Onah Stanley K.
Published: November 2019

Extended-spectrum β-lactamase (ESBL)-producing organisms have become a serious challenge in healthcare delivery globally. The prevalence of ESBL carriage in healthy and sick children in Enugu, Nigeria, was bacteriologically investigated in this study. Four hundred and twenty-two biological samples (mid-stream urine and feces) were bacteriologically analyzed. The isolates were screened for ESBL production using Clinical and Laboratory Standards Institute (CLSI) breakpoints. The suspected ESBL producers were confirmed using double disc synergy test method. Out of the 162 isolates screened, 32 (19.8%) were confirmed as ESBL positive, with a prevalence of 25.32% among sick children in Enugu State University Teaching Hospital (ESUTH), Parklane, Enugu and 13.89% in apparently healthy children in a community setting. Klebsiella spp. and Escherichia coli had the highest prevalence of 34.6% and 28.6%, respectively; Citrobacter spp. and Enterobacter spp. were 18.2% and 16.7%, respectively. The ESBL positive isolates were resistant to sulfamethoxazole/trimethoprim (100%), tetracycline (100%), kanamycin (96.9%), nitrofurantoin (84.4%), ciprofloxacin (68.6%), and chloramphenicol (62.5%) but susceptible to meropenem (100%), colistin (56.3%), and gentamicin (50%). Klebsiella spp. had the highest ESBL occurrence among sick children while E. coli had the highest ESBL occurrence among healthy children in Enugu. All ESBL-positive isolates were multiply resistant to conventional antibiotics. The emergence and spread of β-lactamase-producing Enterobacteriaceae in hospital and community environments highlight the possibility for an infection outbreak if not checked.
Refined Microstructure and Enhanced Hardness in Friction Stir-Welded AZ31 Magnesium Alloy Induced by Heat Pipe with Different Cooling Liquid
Authors: Zhang Yu-Meng , Chen Liang-Yu , Lu Sheng , Zhao Cuihua , Wang Yi-Hao
Published: November 2019

The temperature field in welded plates has a significant influence on the microstructure and thereby their properties during friction stir welding (FSW). In this work, a self-designed heat pipe with different cooling liquid was applied in the FSW process for AZ31 magnesium alloy. The temperature fields, microstructures and properties of the welded joints were investigated. The peak temperatures and the durations of high temperature at both the advancing side and the retreating side decrease during the FSW process after applying the heat pipe and adding the ambient temperature water in the condensing tank. The top part of the weld nugget zone of the joint shows a significant decrease as well as its middle part due to the cooling effect of the heat pipe. The microstructure of the weld nugget zone is refined, associated with the increase in the hardness after applying the heat pipe. When the cooling liquid turns into ice water, grains in the weld nugget zone become significantly smaller and have a more homogeneous size. The mean value of hardness increases and the corresponding deviation is declined. Therefore, these results indicate that the application of the heat pipe and the employment of ice water as the cooling liquid can further refine the microstructure and enhance the strength of the material.
Critical Raw Materials Recovery through Bio/Hydrometallurgy from Secondary Resources
Authors: Sethurajan Manivannan , van Hullebusch Eric D.
Published: November 2019

Demand for critical raw materials (CRMs) to be used in consumer products is growing rapidly [...]
A New Classification Method of Metastatic Cancers Using a 1H-NMR-Based Approach: A Study Case of Melanoma, Breast, and Prostate Cancer Cell Lines
Authors: Schepkens Corentin , Dallons Matthieu , Dehairs Jonas , Talebi Ali , Jeandriens Jérôme , Drossart Lise-Marie , Auquier Guillaume , Tagliatti Vanessa , Swinnen Johannes V. , Colet Jean-Marie
Published: November 2019

In this study, metastatic melanoma, breast, and prostate cancer cell lines were analyzed using a 1H-NMR-based approach in order to investigate common features and differences of aggressive cancers metabolomes. For that purpose, 1H-NMR spectra of both cellular extracts and culture media were combined with multivariate data analysis, bringing to light no less than 20 discriminant metabolites able to separate the metastatic metabolomes. The supervised approach succeeded in classifying the metastatic cell lines depending on their glucose metabolism, more glycolysis-oriented in the BRAF proto-oncogene mutated cell lines compared to the others. Other adaptive metabolic features also contributed to the classification, such as the increased total choline content (tCho), UDP-GlcNAc detection, and various changes in the glucose-related metabolites tree, giving additional information about the metastatic metabolome status and direction. Finally, common metabolic features detected via 1H-NMR in the studied cancer cell lines are discussed, identifying the glycolytic pathway, Kennedy’s pathway, and the glutaminolysis as potential and common targets in metastasis, opening up new avenues to cure cancer.
Robust Tracking of a Cost-Effective Micro-Stereolithography System Based on a Compliant Nanomanipulator
Authors: Cao Yue , Zhang Zhen
Published: November 2019

Micro-stereolithography (MSL) has emerged as a promising and challenging technique in micro-/nano-scale additive manufacturing. Besides the requirement of the light source, the motion system requires ultra-high-precision tracking capability to reach the right location for every solidification event. To achieve single-digit micron feature size of the fabrication, we propose a robust control strategy to support a self-developed cost-effective MSL prototype based on a compliant nanomanipulator and a blue light-emitting diode (LED) module. In particular, the nonlinearity and parameter-variation of the compliant manipulator are dealt with by a robust radial basis function (RBF)-based neural network, and the repetitive control (RC) is innovatively integrated with RBF to improve the tracking performance of a closed pattern. Various simulations and real-time experiments are conducted to validate the proposed control strategy. The fabrication of a closed pattern will not begin by turning on the laser source until the tracking error reaches submicrons, and the fabrication results demonstrate that the cost-effective MSL system is capable of fabricating 2.5 µm feature size in a 0.5 mm working range.
The Analysis of the Influence of Threshold on the Dynamic Contact Process of a Fabricated Vertically Driven MEMS Inertial Switch
Authors: Chen Wenguo , Wang Rui , Wang Huiying , Kong Dejian , Sun Shulei
Published: November 2019

In this work, to evaluate the influence of the threshold on the dynamic contact process, five models (number 1, 2, 3, 4, 5) with different thresholds were proposed and fabricated with surface micromachining technology. The contact time and response time were used to characterize the dynamic contact performance. The dynamic contact processes of the inertial switches with gradually increasing thresholds were researched using analytical, simulation, and experimental methods. The basic working principle analysis of the inertial switch shows that the contact time of the inertial switch with a low-g value can be extended by using a simply supported beam as the fixed electrode, but the high-G inertial needs more elasticity for fixed electrode. The simulation results indicate that the response time and contact time decrease with the increment in the designed threshold. Prototypes were tested using a dropping hammer system, and the test result indicates that the contact time of the inertial switch with a fixed electrode of the simply supported beam is about 15 and 5 μs when the threshold is about 280 and 580 g, respectively. Meanwhile, the contact time can be extended to 100 μs for the inertial switch using a spring as the fixed electrode when the threshold is about 280 and 580 g. These test results not only prove that the spring fixed electrode can effectively extend the contact time, but also prove that the style of the fixed electrode is the deciding factor affecting the contact time of the high-G inertial switch.
The Effects of Flavomycin and Colistin Sulfate Pre-Treatment on Ileal Bacterial Community Composition, the Response to Salmonella typhimurium and Host Gene Expression in Broiler Chickens
Authors: He Yang , Yang Yanyan , Dong Yuanyang , Yan Changliang , Zhang Bingkun
Published: November 2019

The composition of the bacterial community affects the intestinal health and growth performance of broiler chickens. The main purpose of this study was to explore the effects of flavomycin and colistin sulfate on the resistance to Salmonella typhimurium infection, ileal bacteria and intestinal health. In total, 396 1-day-old broiler chickens were randomly divided into six groups. Two groups were fed each one of the diets—the control diet (CON), the flavomycin at 10 mg/kg diet (AntiG+), and the colistin sulfate at 40 mg/kg diet (AntiG−), for 5 days. Then, one of each of the two groups was challenged with S. typhimurium on the 8th day; these were named CONS, AntiG+S and AntiG−S, respectively. The results showed that S. typhimurium significantly reduced the feed intake and body weight gain, and increased the feed conversion ratio (p < 0.05). It also increased the inflammatory expressions of NF-κB and MyD88 genes (p < 0.05); and reduced the expressions of claudin-1, occludin and mucin-2 (p < 0.05) tight junction genes in the intestines. S. typhimurium significantly reduced ileal bacterial diversity indexes of observed-species, chao1 and Shannon (p < 0.05). Compared with AntiG+S group, AntiG−S group increased the body weight gain of broiler chickens (p < 0.05), reduced the expression of inflammatory genes (p < 0.05) and intestinal permeability to fluorescein isothiocyanate (p < 0.05). AntiG-S group also improved the ileal bacterial diversity indexes of observed-species and Shannon (p < 0.05). There were many significant correlations between intestinal bacteria, intestinal gene expressions and intestinal morphology (p < 0.05). This study indicated that pre-constructed AntiG− bacteria could against a S. typhimurium infection by inhibiting the expressions of intestinal inflammation genes and increasing the diversity of intestinal bacteria.
Recycling Lead–Zinc Tailings for Cemented Paste Backfill and Stabilisation of Excessive Metal
Authors: Su Zhu , Chen Qiusong , Zhang Qinli , Zhang Deming
Published: November 2019

This study demonstrates the feasibility of recycling lead–zinc tailing (LZT) as a cemented paste backfill (CPB) by considering the mechanical properties and environmental effects, thus providing an approach for safe and environmentally friendly treatment of LZT. First, the mechanical properties of CPB samples were tested. When the cement/tailing ratio was 1:6 and the slurry concentration was 70%, the maximum unconfined compressive strength (UCS) of the CPB cured for 28 days reaching 2.05 MPa, which could ensure safe mining. Then, the metals with pollution potential in the backfill slurry were investigated through static leaching. Finally, after adding immobilisation materials to stabilise excessive metals, the environmental stability of the CPB was demonstrated through dynamic leaching and a toxicity characteristic leaching procedure. The results show that the lead leached from the backfill slurry still exceeds the Chinese standard for groundwater quality (GB/T14848-2017 Class III). The addition of 2 mg/L polyaluminium sulfate (PAS) can further improve the strength of the CPB and maintain the environmental friendliness of the CPB. Therefore, the technology of recovering LZT as a CPB proposed in this study is an effective alternative to deal with LZT, which can help lead–zinc mines meet the requirements of cleaner production.
The Environmental Significance of Sediment Surface Area as a Controlling Factor in the Preservation of Polychlorinated Dibenzo-P-Dioxins and Dibenzofurans (PCDD/PCDF) in Sediments Adjacent to Woodfibre Pulp Mill, Howe Sound, British Columbia
Authors: Chalmers Gareth , Adams Rupert , Bustin Amanda , Bustin Marc
Published: November 2019

A sediment core was retrieved from an area adjacent to a Pulp and Paper Mill in Howe Sound, British Columbia, in order to examine the accumulation dioxins (PCDDs) and furans (PCDFs). Downcore distribution of TOC in the bulk samples is relatively uniform (0.5–1.7 wt. %). Bulk PCDD/F concentration shows selective enrichment and depletion at specific sediment horizons, and a low to moderate correlation with surface area (r2 = 0.23–0.54). TOC in size fractionated sediments ranges from 0.3–11 wt. % and shows a moderate correlation with surface area (r2 = 0.51). The relationship between PCDD/Fs and surface area is congener specific, ranging from no significant correlation (TCDD; r2 = 0.05), to a good correlation (i.e., OCDF; r2 = 0.74). Results indicate that both dioxin and furan concentrations are related to organic matter concentration, molecular chlorination and sediment surface area.
C- and N-Bearing Species in Reduced Fluids in the Simplified C–O–H–N System and in Natural Pelite at Upper Mantle P–T Conditions
Authors: Sokol Ivan , Sokol Alexander , Bul’bak Taras , Nefyodov Andrey , Zaikin Pavel , Tomilenko Anatoly
Published: November 2019

C- and N-bearing species in reduced fluids weree studied experimentally in C–O–H–N and muscovite–C–O–H–N systems and in natural carbonate-bearing samples at mantle P–T parameters. The experiments reproduced three types of reactions leading to formation of hydrocarbons (HCs) at 3.8–7.8 GPa and 800–1400 °C and at hydrogen fugacity (fH2) buffered by the Fe–FeO (IW) + H2O or Mo–MoO2 (MMO) + H2O equilibria: (i) Thermal destruction of organic matter during its subduction into the mantle (with an example of docosane), (ii) hydrogenation of graphite upon interaction with H2-enriched fluids, and (iii) hydrogenation of carbonates and products of their reduction in metamorphic clayey rocks. The obtained quenched fluids analyzed after the runs by gas chromatography-mass spectrometry (GC–MS) and electronic ionization mass-spectrometry (HR–MS) contain CH4 and C2H6 as main carbon species. The concentrations of C2-C4 alkanes in the fluids increase as the pressure and temperature increase from 3.8 to 7.8 GPa and from 800 to 1400 °C, respectively. The fluid equilibrated with the muscovite–garnet–omphacite–kyanite–rutile ± coesite assemblage consists of 50–80 rel.% H2O and 15–40 rel.% alkanes (C1 > C2 > C3 > C4). Main N-bearing species are ammonia (NH3) in the C–O–H–N and muscovite–C–O–H–N systems or methanimine (CH3N) in the fluid derived from the samples of natural pelitic rocks. Nitrogen comes either from air or melamine (C3H6N6) in model systems or from NH4+ in the runs with natural samples. The formula CH3N in the quenched fluid of the C–O–H–N system is confirmed by HR–MS. The impossibility of CH3N incorporation into K-bearing silicates because of a big CH3NH+ cation may limit the solubility of N in silicates at low fO2 and hence may substantially influence the mantle cycle of nitrogen. Thus, subduction of slabs containing carbonates, organic matter, and N-bearing minerals into strongly reduced mantle may induce the formation of fluids enriched in H2O, light alkanes, NH3, and CH3N. The presence of these species must be critical for the deep cycles of carbon, nitrogen, and hydrogen.
Effect of Heterointerface on NO2 Sensing Properties of In-Situ Formed TiO2 QDs-Decorated NiO Nanosheets
Authors: Wu Congyi , Zhang Jian , Wang Xiaoxia , Xie Changsheng , Shi Songxin , Zeng Dawen
Published: November 2019

In this work, TiO2 QDs-modified NiO nanosheets were employed to improve the room temperature NO2 sensing properties of NiO. The gas sensing studies showed that the response of nanocomposites with the optimal ratio to 60 ppm NO2 was nearly 10 times larger than that of bare NiO, exhibiting a potential application in gas sensing. Considering the commonly reported immature mechanism that the effective charge transfer between two phases contributes to an enhanced sensitivity, the QDs sensitization mechanism was further detailed by designing a series of contrast experiments. First, the important role of the QDs size effect was revealed by comparing the little enhanced sensitivity of TiO2 particle-modified NiO with the largely enhanced sensitivity of TiO2 QDs-NiO. Second, and more importantly, direct evidence of the heterointerface charge transfer efficiency was detailed by the extracted interface bond (Ti-O-Ni) using XPS peak fitting. This work can thus provide guidelines to design more QDs-modified nanocomposites with higher sensitivity for practical applications.
Fractal Silver Dendrites as 3D SERS Platform for Highly Sensitive Detection of Biomolecules in Hydration Conditions
Authors: Lo Faro Maria José , D’Andrea Cristiano , Leonardi Antonio Alessio , Morganti Dario , Irrera Alessia , Fazio Barbara
Published: November 2019

In this paper, we report on the realization of a highly sensitive and low cost 3D surface-enhanced Raman scattering (SERS) platform. The structural features of the Ag dendrite network that characterize the SERS material were exploited, attesting a remarked self-similarity and scale invariance over a broad range of length scales that are typical of fractal systems. Additional structural and optical investigations confirmed the purity of the metal network, which was characterized by low oxygen contamination and by broad optical resonances introduced by the fractal behavior. The SERS performances of the 3D fractal Ag dendrites were tested for the detection of lysozyme as probe molecule, attesting an enhancement factor of ~2.4 × 106. Experimental results assessed the dendrite material as a suitable SERS detection platform for biomolecules investigations in hydration conditions.
The Growth of Graphene on Ni–Cu Alloy Thin Films at a Low Temperature and Its Carbon Diffusion Mechanism
Authors: Dong Yibo , Guo Sheng , Mao Huahai , Xu Chen , Xie Yiyang , Cheng Chuantong , Mao Xurui , Deng Jun , Pan Guanzhong , Sun Jie
Published: November 2019

Carbon solid solubility in metals is an important factor affecting uniform graphene growth by chemical vapor deposition (CVD) at high temperatures. At low temperatures, however, it was found that the carbon diffusion rate (CDR) on the metal catalyst surface has a greater impact on the number and uniformity of graphene layers compared with that of the carbon solid solubility. The CDR decreases rapidly with decreasing temperatures, resulting in inhomogeneous and multilayer graphene. In the present work, a Ni–Cu alloy sacrificial layer was used as the catalyst based on the following properties. Cu was selected to increase the CDR, while Ni was used to provide high catalytic activity. By plasma-enhanced CVD, graphene was grown on the surface of Ni–Cu alloy under low pressure using methane as the carbon source. The optimal composition of the Ni–Cu alloy, 1:2, was selected through experiments. In addition, the plasma power was optimized to improve the graphene quality. On the basis of the parameter optimization, together with our previously-reported, in-situ, sacrificial metal-layer etching technique, relatively homogeneous wafer-size patterned graphene was obtained directly on a 2-inch SiO2/Si substrate at a low temperature (~600 °C).
Synthesis of Mn0.5Zn0.5SmxEuxFe1.8−2xO4 Nanoparticles via the Hydrothermal Approach Induced Anti-Cancer and Anti-Bacterial Activities
Authors: Akhtar Sultan , Rehman Suriya , Almessiere Munirah A. , Khan Firdos  Alam , Slimani Yassine , Baykal Abdulhadi
Published: November 2019

Manganese metallic nanoparticles are attractive materials for various biological and medical applications. In the present study, we synthesized unique Mn0.5Zn0.5SmxEuxFe1.8−2xO4 (0.01 ≤ x ≤ 0.05) nanoparticles (NPs) by using the hydrothermal approach. The structure and surface morphology of the products were determined by X-ray powder diffraction (XRD), transmission electron and scanning electron microcopies (TEM and SEM), along with energy dispersive X-ray spectroscopy (EDX). We evaluated the impact of Mn0.5Zn0.5SmxEuxFe1.8−2xO4 NPs on both human embryonic stem cells (HEK-293) (normal cells) and human colon carcinoma cells (HCT-116) (cancerous cells). We found that post-48 h of treatment of all products showed a significant decline in the cancer cell population as revealed by microscopically and the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) assay. The inhibitory concentration (IC50) values of the products ranged between 0.75 and 2.25 µg/mL. When tested on normal and healthy cells (HEK-293), we found that the treatment of products did not produce any effects on the normal cells, which suggests that all products selectively targeted the cancerous cells. The anti-bacterial properties of the samples were also evaluated by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays, which showed that products also inhibited the bacterial growth.
One-Step Hydrothermal Synthesis of P25 @ Few Layered MoS2 Nanosheets toward Enhanced Bi-catalytic Activities: Photocatalysis and Electrocatalysis
Authors: Zhou Fang , Zhang Zhiguang , Wang Zhihua , Wang Yajing , Xu Liping , Wang Qiang , Liu Wenjun
Published: November 2019

P25 loaded few layered molybdenum disulfide (MoS2) nanosheets (P25@MoS2) are successfully synthesized through a facile one-step hydrothermal process. The bi-catalytic activities, i.e., photocatalytic and electrocatalytic activities, of the as-prepared nanomaterials have been investigated. For the as-prepared products, the photocatalytic performances were investigated by degrading simulated pollutant under sunlight irradiation, and the hydrogen evolution reaction evaluated the electrocatalytic performances. The results indicate that P25@MoS2 possesses excellent activities in both photocatalysis and electrocatalysis. The presence of MoS2 broadens the light absorption range of P25 and improves the separation and transformation efficiency of photogenerated carriers, thus improving its photocatalytic performance. The existence of P25 inhibits the aggregation of MoS2 to form more dispersed MoS2 nanosheets with only few layers increasing its active sites. Thereby, the electrocatalytic performance is heightened. The excellent multifunction makes the as-prepared P25@MoS2 a promising material in the fields of environment and energy.
Daily Ingestion of Eggplant Powder Improves Blood Pressure and Psychological State in Stressed Individuals: A Randomized Placebo-Controlled Study
Authors: Nishimura Mie , Suzuki Miho , Takahashi Ryuto , Yamaguchi Shohei , Tsubaki Kazufumi , Fujita Tomoyuki , Nishihira Jun , Nakamura Kozo
Published: November 2019

Eggplant (Solanum melongena) is a globally popular vegetable and its significant health effect has not been reported in randomized controlled trials. Recently, we reported that eggplant was rich in choline esters, including acetylcholine (ACh), and had an antihypertensive effect in spontaneously hypertensive rats. Here, we evaluated the effects of a continuous intake of eggplant powder on blood pressure (BP), stress, and psychological state (PS) in 100 stressed participants with normal-high BP or grade 1 hypertension in a randomized, double-blind, placebo-controlled, parallel-group comparative study. The participants were randomly assigned to the eggplant or placebo group. Participants in the eggplant group ingested capsules containing eggplant powder (1.2 g/day; 2.3 mg of ACh/day) for 12 weeks, whereas participants in the placebo group ingested placebo capsules. The primary outcome assessed was hospital BP. Secondary outcomes were stress and PS. Eggplant powder intake significantly decreased the hospital diastolic blood pressure (DBP) at week 8 overall and in the normal-high BP group, and the systolic blood pressure (SBP) and DBP at week 12 overall and in the grade 1 hypertension group, compared to those of the placebo group. It also improved negative PSs at week 8 or 12 in the normal-high BP group. This is the first evidence of the BP- and PS-improving effects of eggplant intake in humans. The functional substance responsible for the effects was estimated to be eggplant-derived choline ester, namely ACh.
Patient Involvement in Education of Nutrition and Dietetics Students: A Systematic Review
Authors: Porter Judi , Kellow Nicole , Anderson Amanda , Bryce Andrea , Dart Janeane , Palermo Claire , Volders Evelyn , Gibson Simone
Published: November 2019

A client-centred approach sits at the core of modern healthcare. Exploration of the patients’ role within the education of nutrition and dietetic students has not previously been undertaken. This review aimed to synthesise the learning outcomes that result from involvement of patients in nutrition and dietetic student education, and to consider whether these interactions promote patient-centred care. Five electronic databases were searched, supported by hand-searching of references of included studies. Screening of title/abstract and then full text papers was undertaken; key characteristics and outcomes were extracted and synthesised narratively. The likely impact of interventions was evaluated using Kirkpatrick’s Hierarchy; study quality was assessed using the Medical Education Research Study Quality Instrument and Critical Appraisal Skills Programme checklist. Of 7436 studies identified through database searching, and one additional study located through hand searching of reference lists, the final library consisted of 13 studies. All studies reported benefits for student learning from patient involvement, while one paper identified patient benefits from student interventions. Patients as recipients of care mostly contributed in a passive role in student education activities. Quality assessment identified methodological limitations in most studies. Patient involvement in the education of dietitians supports skill development and therefore progression to professional practice. Although nutrition and dietetics education has a focus on client-centred care, the translation of these concepts into an interactive student educational experience has been investigated to a limited extent. Collaboration with patients in student education is an area for further development.
Low-Carbohydrate Diet Inhibits Different Advanced Glycation End Products in Kidney Depending on Lipid Composition but Causes Adverse Morphological Changes in a Non-Obese Model Mice
Authors: Kaburagi Tomoko , Kanaki Kazuma , Otsuka Yuko , Hino Rumi
Published: November 2019

Low carbohydrate diets (LC diets) have been noted for adverse health effects. In addition, the effect of lipid composition on an LC diet is unclear. In this study, we used an LC diet containing two different lipids, lard (LC group) and medium-chain triglyceride oil (MCT-LC group), to examine the effect of an LC diet in non-obese mice. Male C57BL/6J mice were fed the control diet or one of the experimental diets ad libitum for 13 weeks. Increased renal weight and glomerular hypertrophy, as well as enlargement of intraglomerular small vessels with wall thickening, were seen in the LC and MCT-LC groups. Renal AMP-activated protein kinase activity was significantly decreased only in the LC diet group. On the other hand, epididymal adipose tissue weight and adipocyte area were markedly decreased only in the MCT-LC group. A positive effect was also observed in the kidney, where different advanced glycation end products, Nε-(carboxyethyl)-lysine and Nε-(carboxymethyl)-lysine, were inhibited depending on the lipid composition of the LC diet. Our findings suggest that, in non-obese conditions, low dietary intake of carbohydrates had both positive and negative impacts. The safety of diets low in carbohydrates, including the effects of fatty acid composition, requires further investigation.
Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
Authors: Sasaki Hiroyuki , Miyakawa Hiroki , Watanabe Aya , Nakayama Yuki , Lyu Yijin , Hama Koki , Shibata Shigenobu
Published: November 2019

Water-soluble dietary fiber is known to modulate fecal microbiota. Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota. Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota. Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening. Then, 10–14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured. The microbiome was determined using 16S rDNA sequencing. Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition. These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period. Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule. When the duration of fasting was equal, the difference between morning and evening nearly disappeared. Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.
DNA Methylation Signatures of Breastfeeding in Buccal Cells Collected in Mid-Childhood
Authors: Odintsova Veronika V. , Hagenbeek Fiona A. , Suderman Matthew , Caramaschi Doretta , van Beijsterveldt Catharina E. M. , Kallsen Noah A. , Ehli Erik A. , Davies Gareth E. , Sukhikh Gennady T. , Fanos Vassilios , Relton Caroline , Bartels Meike , Boomsma Dorret I. , van Dongen Jenny
Published: November 2019

Breastfeeding has long-term benefits for children that may be mediated via the epigenome. This pathway has been hypothesized, but the number of empirical studies in humans is small and mostly done by using peripheral blood as the DNA source. We performed an epigenome-wide association study (EWAS) in buccal cells collected around age nine (mean = 9.5) from 1006 twins recruited by the Netherlands Twin Register (NTR). An age-stratified analysis examined if effects attenuate with age (median split at 10 years; n<10 = 517, mean age = 7.9; n>10 = 489, mean age = 11.2). We performed replication analyses in two independent cohorts from the NTR (buccal cells) and the Avon Longitudinal Study of Parents and Children (ALSPAC) (peripheral blood), and we tested loci previously associated with breastfeeding in epigenetic studies. Genome-wide DNA methylation was assessed with the Illumina Infinium MethylationEPIC BeadChip (Illumina, San Diego, CA, USA) in the NTR and with the HumanMethylation450 Bead Chip in the ALSPAC. The duration of breastfeeding was dichotomized (‘never‘ vs. ‘ever’). In the total sample, no robustly associated epigenome-wide significant CpGs were identified (α = 6.34 × 10–8). In the sub-group of children younger than 10 years, four significant CpGs were associated with breastfeeding after adjusting for child and maternal characteristics. In children older than 10 years, methylation differences at these CpGs were smaller and non-significant. The findings did not replicate in the NTR sample (n = 98; mean age = 7.5 years), and no nearby sites were associated with breastfeeding in the ALSPAC study (n = 938; mean age = 7.4). Of the CpG sites previously reported in the literature, three were associated with breastfeeding in children younger than 10 years, thus showing that these CpGs are associated with breastfeeding in buccal and blood cells. Our study is the first to show that breastfeeding is associated with epigenetic variation in buccal cells in children. Further studies are needed to investigate if methylation differences at these loci are caused by breastfeeding or by other unmeasured confounders, as well as what mechanism drives changes in associations with age.
Vitamin D Ameliorates Fat Accumulation with AMPK/SIRT1 Activity in C2C12 Skeletal Muscle Cells
Authors: Chang Eugene , Kim Yangha
Published: November 2019

Excessive fat accumulation has been considered as a major contributing factor for muscle mitochondrial dysfunction and its associated metabolic complications. The purpose of present study is to investigate a role of vitamin D in muscle fat accumulation and mitochondrial changes. In differentiated C2C12 muscle cells, palmitic acid (PA) was pretreated, followed by incubation with 1,25-dihyroxyvitamin D (1,25(OH)2D) for 24 h. PA led to a significant increment of triglyceride (TG) levels with increased lipid peroxidation and cellular damage, which were reversed by 1,25(OH)2D. The supplementation of 1,25(OH)2D significantly enhanced PA-decreased mtDNA levels as well as mRNA levels involved in mitochondrial biogenesis such as nuclear respiratory factor 1 (NRF1), peroxisome proliferative activated receptor gamma coactivator-1α (PGC-1α), and mitochondrial transcription factor A (Tfam) in C2C12 myotubes. Additionally, 1,25(OH)2D significantly increased ATP levels and gene expression related to mitochondrial function such as carnitine palmitoyltransferase 1 (CPT1), peroxisome proliferator-activated receptor α (PPARα), very long-chain acyl-CoA dehydrogenase (VLCAD), long-chain acyl-CoA dehydrogenase (LCAD), medium-chain acyl-CoA dehydrogenase (MCAD), uncoupling protein 2 (UCP2), and UCP3 and the vitamin D pathway including 25-dihydroxyvitamin D3 24-hydroxylase (CYP24) and 25-hydroxyvitamin D3 1-alpha-hydroxylase (CYP27) in PA-treated C2C12 myotubes. In addition to significant increment of sirtuin 1 (SIRT1) mRNA expression, increased activation of adenosine monophosphate-activated protein kinase (AMPK) and SIRT1 was found in 1,25(OH)2D-treated C2C12 muscle cells. Thus, we suggest that the observed protective effect of vitamin D on muscle fat accumulation and mitochondrial dysfunction in a positive manner via modulating AMPK/SIRT1 activation.
Trends in Food Consumption Patterns of US Infants and Toddlers from Feeding Infants and Toddlers Studies (FITS) in 2002, 2008, 2016
Authors: Duffy Emily W. , Kay Melissa C. , Jacquier Emma F. , Catellier Diane , Hampton Joel , Anater Andrea S. , Story Mary
Published: November 2019

The Feeding Infants and Toddlers Study (FITS) is the largest survey of dietary intake among infants and young children in the United States. Dietary patterns in early childhood are a key component of prevention of diet-related chronic diseases, yet little is known about how food consumption patterns of infants and young children have changed over time. The objective of this study is to examine trends in food and beverage consumption among children ages 6–23.9 months using data from the FITS conducted in 2002, 2008, and 2016. A total of 5963 infants and young children ages 6–23.9 months were included in these analyses. Food consumption data were collected using a multiple-pass 24-h recall by telephone using the Nutrition Data System for Research. Linear trends were assessed using the Wald’s test in a multivariable linear regression model. Positive significant findings include increases in breast milk consumption and decreases in the consumption of sweets, sugar-sweetened beverages, and 100% fruit juice. More troubling findings include decreasing infant cereal consumption, stagnant or decreasing whole grain consumption, and stagnant consumption of vegetables. Our findings suggest some promising improvements in dietary intake among infants and toddlers in the United States over the past 15 years, but further policy, programmatic, and industry efforts are still needed.
Genetic Deletion of Syndecan-4 Alters Body Composition, Metabolic Phenotypes, and the Function of Metabolic Tissues in Female Mice Fed A High-Fat Diet
Authors: De Luca Maria , Vecchie’ Denise , Athmanathan Baskaran , Gopalkrishna Sreejit , Valcin Jennifer A. , Swain Telisha M. , Sertie Rogerio , Wekesa Kennedy , Rowe Glenn C. , Bailey Shannon M. , Nagareddy Prabhakara R.
Published: November 2019

Syndecans are transmembrane proteoglycans that, like integrins, bind to components of the extracellular matrix. Previously, we showed significant associations of genetic variants in the Syndecan-4 (SDC4) gene with intra-abdominal fat, fasting plasma glucose levels, and insulin sensitivity index in children, and with fasting serum triglyceride levels in healthy elderly subjects. An independent study also reported a correlation between SDC4 and the risk of coronary artery disease in middle-aged patients. Here, we investigated whether deletion of Sdc4 promotes metabolic derangements associated with diet-induced obesity by feeding homozygous male and female Sdc4-deficient (Sdc4-/-) mice and their age-matched wild-type (WT) mice a high-fat diet (HFD). We found that WT and Sdc4-/- mice gained similar weight. However, while no differences were observed in males, HFD-fed female Sdc4-/- mice exhibited a higher percentage of body fat mass than controls and displayed increased levels of plasma total cholesterol, triglyceride, and glucose, as well as reduced whole-body insulin sensitivity. Additionally, they had an increased adipocyte size and macrophage infiltration in the visceral adipose tissue, and higher triglyceride and fatty acid synthase levels in the liver. Together with our previous human genetic findings, these results provide evidence of an evolutionarily conserved role of SDC4 in adiposity and its complications.
Rural–Urban Differences in Dietary Behavior and Obesity: Results of the Riskesdas Study in 10–18-Year-Old Indonesian Children and Adolescents
Authors: Nurwanti Esti , Hadi Hamam , Chang Jung-Su , Chao Jane C.-J. , Paramashanti Bunga Astria , Gittelsohn Joel , Bai Chyi-Huey
Published: November 2019

Obesity has become a significant problem for developing countries, including Indonesia. High duration of sedentary activity and high intake of unhealthy foods were associated with high risk of overweight and obesity. The objective of this study was to compare the distributions of sedentary activity and dietary behavior with overweight/obesity risks between urban and rural areas among children and adolescents aged 10–18 years in Indonesia. This is a cross-sectional study. Data from a national survey in 33 Indonesian provinces (Basic Health Research /Riskesdas 2013) were analyzed. Multiple logistic regression models were used to calculate the odds ratio (OR) adjusted with all variables, such as age, gender, residency, education level, physical activity, and food intake. An urban–rural residence difference was found in the factors related to obesity. Daily caffeinated soft drinks and energy drinks consumption (OR = 1.12, 95% CI: 1.01–1.23) were related to risk of overweight and obesity in urban areas. Daily grilled foods (OR = 1.32, 95% CI: 1.22–1.42) and salty food (OR = 1.09, 95% CI: 1.04–1.15) consumption were significantly associated with obesity in rural areas but not in urban areas. Furthermore, sedentary activity was correlated with overweight and obesity among those who lived in urban and rural areas. Our findings suggest that education, environmental, and policy interventions may need to specifically target urban settings, where access is high to a wide range of processed and traditional high-sugar, high-fat snack foods and beverages.
Vitamin B12 Status Upon Short-Term Intervention with a Vegan Diet—A Randomized Controlled Trial in Healthy Participants
Authors: Lederer Ann-Kathrin , Hannibal Luciana , Hettich Manuel , Behringer Sidney , Spiekerkoetter Ute , Steinborn Carmen , Gründemann Carsten , Zimmermann-Klemd Amy Marisa , Müller Alexander , Simmet Thomas , Schmiech Michael , Maul-Pavicic Andrea , Samstag Yvonne , Huber Roman
Published: November 2019

Vegans are at an increased risk for certain micronutrient deficiencies, foremost of vitamin B12. Little is known about the short-term effects of dietary change to plant-based nutrition on vitamin B12 metabolism. Systemic biomarkers of vitamin B12 status, namely, serum vitamin B12 and holotranscobalamin, may respond quickly to a reduced intake of vitamin B12. To test this hypothesis, 53 healthy omnivore subjects were randomized to a controlled unsupplemented vegan diet (VD, n = 26) or meat-rich diet (MD, n = 27) for 4 weeks. Vitamin B12 status was examined by measurement of serum vitamin B12, holotranscobalamin (holo-TC), methylmalonic acid (MMA) and total plasma homocysteine (tHcy). Holo-TC decreased significantly in the VD compared to the MD group after four weeks of intervention, whereas metabolites MMA and tHcy were unaffected. Body weight remained stable in both groups. VD intervention led to a significant reduction of cholesterol intake, and adequate profiles of nutrient and micronutrient status. Lower intake of vitamin B12 was observed in VD, which was mirrored by a lower concentration of serum vitamin B12 and reduced holo-TC after 4 weeks. Plasma holo-TC may be a fast-responding biomarker to monitor adequate supply of vitamin B12 in plant-based individuals.
Apoptosis, Autophagy, and Pyroptosis: Immune Escape Strategies for Persistent Infection and Pathogenesis of Classical Swine Fever Virus
Authors: Ma Sheng-ming , Mao Qian , Yi Lin , Zhao Ming-qiu , Chen Jin-ding
Published: November 2019

Classical swine fever (CSF) is a severe acute infectious disease that results from classical swine fever virus (CSFV) infection, which leads to serious economic losses in the porcine industry worldwide. In recent years, numerous studies related to the immune escape mechanism of the persistent infection and pathogenesis of CSFV have been performed. Remarkably, several independent groups have reported that apoptosis, autophagy, and pyroptosis play a significant role in the occurrence and development of CSF, as well as in the immunological process. Apoptosis, autophagy, and pyroptosis are the fundamental biological processes that maintain normal homeostatic and metabolic function in eukaryotic organisms. In general, these three cellular biological processes are always understood as an immune defense response initiated by the organism after perceiving a pathogen infection. Nevertheless, several viruses, including CSFV and other common pathogens such as hepatitis C and influenza A, have evolved strategies for infection and replication using these three cellular biological process mechanisms. In this review, we summarize the known roles of apoptosis, autophagy, and pyroptosis in CSFV infection and how viruses manipulate these three cellular biological processes to evade the immune response.
Electrostatically Driven Encapsulation of Hydrophilic, Non-Conformational Peptide Epitopes into Liposomes
Authors: Suleiman Ehsan , Damm Dominik , Batzoni Mirjam , Temchura Vladimir , Wagner Andreas , Überla Klaus , Vorauer-Uhl Karola
Published: November 2019

Since the first use of liposomes as carriers for antigens, much work has been done to elucidate the mechanisms involved in the encapsulation of vaccine-relevant biomolecules. However, only a few studies have specifically investigated the encapsulation of hydrophilic, non-conformational peptide epitopes. We performed comprehensive and systematic screening studies, in order to identify conditions that favor the electrostatic interaction of such peptides with lipid membranes. Moreover, we have explored bi-terminal sequence extension as an approach to modify the isoelectric point of peptides, in order to modulate their membrane binding behavior and eventually shift/expand the working range under which they can be efficiently encapsulated in an electrostatically driven manner. The findings of our membrane interaction studies were then applied to preparing peptide-loaded liposomes. Our results show that the magnitude of membrane binding observed in our exploratory in situ setup translates to corresponding levels of encapsulation efficiency in both of the two most commonly employed methods for the preparation of liposomes, i.e., thin-film hydration and microfluidic mixing. We believe that the methods and findings described in the present studies will be of use to a wide audience and can be applied to address the ongoing relevant issue of the efficient encapsulation of hydrophilic biomolecules.