bluuebunny/crossref_metadata_2025 huggingface dataset:
Size Distribution of Microparticles: A New Parameter to Predict Acute Lung Injury After Cardiac Surgery With Cardiopulmonary Bypass
Authors: Yuan Hao-Xiang , Liang Kai-Feng , Chen Chao , Li Yu-Quan , Liu Xiao-Jun , Chen Ya-Ting , Jian Yu-Peng , Liu Jia-Sheng , Xu Ying-Qi , Ou Zhi-Jun , Li Yan , Ou Jing-Song
Published: April 2022

Background

Acute lung injury (ALI) is a common complication after cardiac surgery with cardiopulmonary bypass (CPB). No precise way, however, is currently available to predict its occurrence. We and others have demonstrated that microparticles (MPs) can induce ALI and were increased in patients with ALI. However, whether MPs can be used to predict ALI after cardiac surgery with CPB remains unknown.

Methods

In this prospective study, 103 patients undergoing cardiac surgery with CPB and 53 healthy subjects were enrolled. MPs were isolated from the plasma before, 12 h after, and 3 d after surgery. The size distributions of MPs were measured by the LitesizerTM 500 Particle Analyzer. The patients were divided into two subgroups (ALI and non-ALI) according to the diagnosis of ALI. Descriptive and correlational analyzes were conducted between the size distribution of MPs and clinical data.

Results

Compared to the non-ALI group, the size at peak and interquartile range (IQR) of MPs in patients with ALI were smaller, but the peak intensity of MPs is higher. Multivariate logistic regression analysis indicated that the size at peak of MPs at postoperative 12 h was an independent risk factor for ALI. The area under the curve (AUC) of peak diameter at postoperative 12 h was 0.803. The best cutoff value of peak diameter to diagnose ALI was 223.05 nm with a sensitivity of 88.0% and a negative predictive value of 94.5%. The AUC of IQR at postoperative 12 h was 0.717. The best cutoff value of IQR to diagnose ALI was 132.65 nm with a sensitivity of 88.0% and a negative predictive value of 92.5%. Combining these two parameters, the sensitivity reached 92% and the negative predictive value was 96%.

Conclusions

Our findings suggested that the size distribution of MPs could be a novel biomarker to predict and exclude ALI after cardiac surgery with CPB.
When Patients Recover From COVID-19: Data-Driven Insights From Wearable Technologies
Authors: Guo Muzhe , Nguyen Long , Du Hongfei , Jin Fang
Published: April 2022

Coronavirus disease 2019 (COVID-19) is known as a contagious disease and caused an overwhelming of hospital resources worldwide. Therefore, deciding on hospitalizing COVID-19 patients or quarantining them at home becomes a crucial solution to manage an extremely big number of patients in a short time. This paper proposes a model which combines Long-short Term Memory (LSTM) and Deep Neural Network (DNN) to early and accurately classify disease stages of the patients to address the problem at a low cost. In this model, the LSTM component will exploit temporal features while the DNN component extracts attributed features to enhance the model's classification performance. Our experimental results demonstrate that the proposed model achieves substantially better prediction accuracy than existing state-of-art methods. Moreover, we explore the importance of different vital indicators to help patients and doctors identify the critical factors at different COVID-19 stages. Finally, we create case studies demonstrating the differences between severe and mild patients and show the signs of recovery from COVID-19 disease by extracting shape patterns based on temporal features of patients. In summary, by identifying the disease stages, this research will help patients understand their current disease situation. Furthermore, it will also help doctors to provide patients with an immediate treatment plan remotely that addresses their specific disease stages, thus optimizing their usage of limited medical resources.
Towards Next Generation Cleaning Tools: Factors Affecting Cleaning Robot Usage and Proxemic Behaviors Design
Authors: Chen Yuhao , Luo Yue , Hu Boyi
Published: April 2022

Among all healthcare sectors and working processes, the janitorial section is a prominent source of work-related injuries due to its labor-intensive nature and rising need for a hygienic environment, thus requiring extra attention for prevention strategies. Advancement in robotic technology has allowed autonomous cleaning robots to be a viable solution to ease the burden of janitors. To evaluate the application of commercial-grade cleaning robots, a video-based survey was developed and distributed to participants. Results from 117 participants revealed that: 1) participants were less tolerant when their personal space was invaded by humans compared with the cleaning robot, 2) it is better to inform the surrounding humans that the cleaning robot has been sanitized to make them feel safe and comfortable during the pandemic, and 3) to make the interaction more socially acceptable, the cleaning robot should respect human personal space, especially when there is ample space to maneuver. The findings of the present study provide insight into the usage and Proxemic behaviors design of future cleaning robots.
High-TSH Subclinical Hypothyroidism Is Associated With Postoperative Mortality in Acute Type A Aortic Dissection
Authors: Wang Shi-Pan , Xue Yuan , Li Hai-Yang , Jiang Wen-Jian , Zhang Hong-Jia
Published: April 2022

Background

Subclinical hypothyroidism can negatively affect the cardiovascular system and increase the risk of mortality, especially for individuals with thyroid-stimulating hormone (TSH) levels above 10 mU/L. We investigated the relationship between high-TSH subclinical hypothyroidism and postoperative mortality in acute type A aortic dissection (ATAAD) patients.

Method

We enrolled 146 patients with ATAAD who underwent aortic surgery in Beijing Anzhen Hospital from July 2016 to November 2018. Thyroid hormone levels were obtained before surgery, and participants were divided into a ≥10mU/L TSH level group and a <10mU/L level group. Cox proportional hazard regression and subgroup analysis were conducted to examine the association of preoperative high-TSH subclinical hypothyroidism with postoperative mortality.

Result

Participants with preoperative high-TSH (≥10mU/L) subclinical hypothyroidism tended to have longer hospitalization stays after surgery [16.0 (IQR 11.0-21.0) days vs 12.5 (IQR 8.0-16.0) days, P=0.001]. During the first 30 days after operation, 15 of 146 patients died (10.3%); during a median of 3.16 (IQR 1.76-4.56) years of follow-up, 24 patients died (16.4%). Cox proportional hazard regression showed that preoperative high-TSH subclinical hypothyroidism was independently associated with 30-day mortality (HR=6.2, 95% CI, 1.7-22.0, P=0.005) and postoperative mortality after adjusting for age, sex, BMI, hypertension, ejection fraction, diabetes and history of PCI (HR=3.4, 95% CI, 1.4-8.0, P=0.005).

Conclusion

This study showed that preoperative high-TSH subclinical hypothyroidism was an independent predictor of postoperative mortality in ATAAD patients who underwent aortic surgery.
Serum Linkage-Specific Sialylation Changes Are Potential Biomarkers for Monitoring and Predicting the Recurrence of Papillary Thyroid Cancer Following Thyroidectomy
Authors: Cao Zhen , Zhang Zejian , Liu Rui , Wu Mengwei , Li Zepeng , Xu Xiequn , Liu Ziwen
Published: April 2022

Background

Although papillary thyroid cancer (PTC) could remain indolent, the recurrence rates after thyroidectomy are approximately 20%. There are currently no accurate serum biomarkers that can monitor and predict recurrence of PTC after thyroidectomy. This study aimed to explore novel serum biomarkers that are relevant to the monitoring and prediction of recurrence in PTC using N-glycomics.

Methods

A high-throughput quantitative strategy based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to obtain serum protein N-glycomes of well-differentiated PTC, postoperative surveillance (PS), postoperative recurrence (PR), and matched healthy controls (HC) including linkage-specific sialylation information.

Results

Serum N-glycan traits were found to differ among PTC, PS, PR, and HC. The differentially expressed N-glycan traits consisting of sixteen directly detected glycan traits and seven derived glycan traits indicated the response to surgical resection therapy and the potential for monitoring the PTC. Two glycan traits representing the levels of linkage-specific sialylation (H4N3F1L1 and H4N6F1E1) which were down-regulated in PS and up-regulated in PR showed high potential as biomarkers for predicting the recurrence after thyroidectomy.

Conclusions

To the best of our knowledge, this study provides comprehensive evaluations of the serum N-glycomic changes in patients with PS or PR for the first time. Several candidate serum N-glycan biomarkers including the linkage-specific sialylation have been determined, some of which have potential in the prediction of recurrence in PTC, and others of which can help to explore and monitor the response to initial surgical resection therapy. The findings enhanced the comprehension of PTC.
Could Green Technology Innovation Help Economy Achieve Carbon Neutrality Development–Evidence From Chinese Cities
Authors: Pu Zhengning , Liu Jingyu , Yang Mingyan
Published: April 2022

China’s 12th Five-Year Plan emphasizes green technological advances in energy conservation, which provides a feasible quasi-natural experimental node to study the role of green technological innovation in influencing the achievement of carbon neutrality. The difference-in-difference model examines whether China’s electricity consumption efficiency has improved since the 12th Five-Year Plan and reveals the role of green technology innovation in this process. Specifically, this paper takes 216 cities in China from 2003 to 2016 as the study sample, the midpoint between China’s 11th and 12th Five-Year Plans as the quasi-natural starting point, and uses the top 50 cities in terms of the number of listed companies as the quasi-natural experimental group. The results show that China’s electricity consumption efficiency has improved significantly since the 12th Five-Year Plan, supported by different robustness tests. The mechanism analysis finds that green technology innovation positively affects energy efficiency but is not the best option for cities with many listed companies. Cities with many listed companies can achieve energy savings by adjusting their industrial structure. Energy conservation and emission reduction policies should be formulated according to the city’s situation and give full play to green technology progress and industrial transformation and upgrading, which is of great significance to achieving carbon neutrality.
Assessing the Impacts of Climate Change on Meteorology and Air Stagnation in China Using a Dynamical Downscaling Method
Authors: Hu Anqi , Xie Xiaodong , Gong Kangjia , Hou Yuhui , Zhao Zhan , Hu Jianlin
Published: April 2022

This study utilizes the Weather Research and Forecasting model with a higher resolution (36 km × 36 km) to dynamically downscale the Community Earth System Model results forced by the three representative concentration pathways (RCP) scenarios (RCP4.5, RCP6.0, and RCP8.5) over China. The goal was to compare meteorological fields during the present (2006–2015) and future (2046–2055) climatological periods. An appropriate air stagnation judgment index was selected to explore the effect of climate change on air quality-related meteorological conditions. The results show that the occurrence of wintertime air stagnation over China in the middle of this century (2046–2055) will reduce slightly, with the largest reduction projected under the RCP8.5 scenario (−4 times). However, long-lasting air stagnation events (ASE) are projected to increase in the future, and this increasing trend is more obvious under the RCP8.5 scenario. The projected increase in the long-lasting ASE in different regions of China ranges from 3 to 11 times. Among these, Central China has the largest increase, followed by East and Northeast China, while South China has the lowest increase in ASE. Our results indicate that more attention should be dedicated to extreme pollution events that may potentially be caused by long-lasting air stagnation events in the future.
Planning, Designing, and Managing Green Roofs and Green Walls for Public Health – An Ecosystem Services Approach
Authors: Ode Sang Åsa , Thorpert Petra , Fransson Ann-Mari
Published: April 2022

Installing green roofs and green walls in urban areas is suggested to supply multiple ecosystem services of benefit to human health and well-being. In a three-step literature review, we examined current knowledge on the link between public health and green roofs and green walls. A systematic search identified 69 scientific articles on green roofs/walls with a public health discourse. These articles were categorized according to type of health path covered (reduction of temperature, air pollution, noise or environmental appraisal) and coverage of issues of relevance for strategies on planning, design/construction, and maintenance of green roofs and green walls. Articles identified through the structured search were complemented with reviews (with no explicit public health rationale) covering reduction of noise, temperature, or air pollution and environmental appraisal. Other relevant studies were identified through snowballing. Several of the articles provided guidelines for optimizing the effect of green roofs/walls in supporting ecosystem services and maximizing well-being benefits to support health pathways identified. These included specifications about planning issues, with recommended spatial allocation (locations where people live, sun-exposed for maximum ambient temperature reduction) and with physical access needed for environmental appraisal. Recommendations regarding design parameters covered substrate depth (deeper generally being better), plant choices (more diverse roofs providing more services), and maintenance issues (moist substrate positively correlated with heat reduction).
Brains as Computers: Metaphor, Analogy, Theory or Fact?
Authors: Brette Romain
Published: April 2022

Whether electronic, analog or quantum, a computer is a programmable machine. Wilder Penfield held that the brain is literally a computer, because he was a dualist: the mind programs the brain. If this type of dualism is rejected, then identifying the brain to a computer requires defining what a brain “program” might mean and who gets to “program” the brain. If the brain “programs” itself when it learns, then this is a metaphor. If evolution “programs” the brain, then this is a metaphor. Indeed, in the neuroscience literature, the brain-computer is typically not used as an analogy, i.e., as an explicit comparison, but metaphorically, by importing terms from the field of computers into neuroscientific discourse: we assert that brains compute the location of sounds, we wonder how perceptual algorithms are implemented in the brain. Considerable difficulties arise when attempting to give a precise biological description of these terms, which is the sign that we are indeed dealing with a metaphor. Metaphors can be both useful and misleading. The appeal of the brain-computer metaphor is that it promises to bridge physiological and mental domains. But it is misleading because the basis of this promise is that computer terms are themselves imported from the mental domain (calculation, memory, information). In other words, the brain-computer metaphor offers a reductionist view of cognition (all cognition is calculation) rather than a naturalistic theory of cognition, hidden behind a metaphoric blanket.
Circadian Rhythm Sleep Disorders: Genetics, Mechanisms, and Adverse Effects on Health
Authors: Liu Chaoran , Tang Xiangrong , Gong Zishan , Zeng Wang , Hou Qiao , Lu Renbin
Published: April 2022

Nearly all living organisms, from cyanobacteria to humans, have an internal circadian oscillation with a periodicity of approximately 24 h. In mammals, circadian rhythms regulate diverse physiological processes including the body temperature, energy metabolism, immunity, hormone secretion, and daily sleep-wake cycle. Sleep is tightly regulated by circadian rhythms, whereas a misalignment between the circadian rhythms and external environment may lead to circadian rhythm sleep disorders (CRSD). CRSD includes four main kinds of disorders: the advanced sleep-wake phase disorder (ASPD), the delayed sleep-wake phase disorder (DSPD), the irregular sleep-wake rhythm disorder and the non-24-h sleep-wake rhythm disorder. Recent studies have begun to shed light on the genetic basis of CRSD. Deciphering the genetic codes for ASPD and DSPD has so far been more successful than the other CRSDs, which allow for the development of animal models and understanding of the pathological mechanisms for these disorders. And studies from humans or animal models implicate CRSDs are associated with adverse health consequences, such as cancer and mental disorders. In this review, we will summarize the recent advances in the genetics, underlying mechanisms and the adverse effects on health of ASPD and DSPD.
The “Self-Sacrifice” of ImmuneCells in Sepsis
Authors: Wen Xiaoyue , Xie Bing , Yuan Shiying , Zhang Jiancheng
Published: April 2022

Sepsis is a life-threatening organ dysfunction caused by the host’s malfunctioning response to infection. Due to its high mortality rate and medical cost, sepsis remains one of the world’s most intractable diseases. In the early stage of sepsis, the over-activated immune system and a cascade of inflammation are usually accompanied by immunosuppression. The core pathogenesis of sepsis is the maladjustment of the host’s innate and adaptive immune response. Many immune cells are involved in this process, including neutrophils, mononuclear/macrophages and lymphocytes. The immune cells recognize pathogens, devour pathogens and release cytokines to recruit or activate other cells in direct or indirect manner. Pyroptosis, immune cell-extracellular traps formation and autophagy are several novel forms of cell death that are different from apoptosis, which play essential roles in the progress of sepsis. Immune cells can initiate “self-sacrifice” through the above three forms of cell death to protect or kill pathogens. However, the exact roles and mechanisms of the self-sacrifice in the immune cells in sepsis are not fully elucidated. This paper mainly analyzes the self-sacrifice of several representative immune cells in the forms of pyroptosis, immune cell-extracellular traps formation and autophagy to reveal the specific roles they play in the occurrence and progression of sepsis, also to provide inspiration and references for further investigation of the roles and mechanisms of self-sacrifice of immune cells in the sepsis in the future, meanwhile, through this work, we hope to bring inspiration to clinical work.
The Effect of Hypothermic Machine Perfusion to Ameliorate Ischemia-Reperfusion Injury in Donor Organs
Authors: Knijff Laura W. D. , van Kooten Cees , Ploeg Rutger J.
Published: April 2022

Hypothermic machine perfusion (HMP) has become the new gold standard in clinical donor kidney preservation and a promising novel strategy in higher risk donor livers in several countries. As shown by meta-analysis for the kidney, HMP decreases the risk of delayed graft function (DGF) and improves graft survival. For the liver, HMP immediately prior to transplantation may reduce the chance of early allograft dysfunction (EAD) and reduce ischemic sequelae in the biliary tract. Ischemia-reperfusion injury (IRI), unavoidable during transplantation, can lead to massive cell death and is one of the main causes for DGF, EAD or longer term impact. Molecular mechanisms that are affected in IRI include levels of hypoxia inducible factor (HIF), induction of cell death, endothelial dysfunction and immune responses. In this review we have summarized and discussed mechanisms on how HMP can ameliorate IRI. Better insight into how HMP influences IRI in kidney and liver transplantation may lead to new therapies and improved transplant outcomes.
Low Intestinal IL22 Associates With Increased Transplant-Related Mortality After Allogeneic Stem Cell Transplantation
Authors: Ghimire Sakhila , Ederer Katharina U. , Meedt Elisabeth , Weber Daniela , Matos Carina , Hiergeist Andreas , Zeman Florian , Wolff Daniel , Edinger Matthias , Poeck Hendrik , Herr Wolfgang , Gessner André , Holler Ernst , Bülow Sigrid
Published: April 2022

The role of IL-22 in adult patients undergoing allogeneic stem cell transplantation (SCT) is of major interest since animal studies showed a protective and regenerative effect of IL-22 in graft versus host disease (GvHD). However, no clinical data exist on the tissue expression. Here we demonstrate that patients not suffering from transplant-related mortality (TRM) show significantly upregulated IL22 expression during histological and clinical GI-GvHD (p = 0.048 and p = 0.022, respectively). In contrast, in GvHD patients suffering from TRM, IL22 was significantly lower (p = 0.007). Accordingly, lower IL22 was associated with a higher probability of TRM in survival analysis (p = 0.005). In a multivariable competing risk Cox regression analysis, low IL22 was identified as an independent risk factor for TRM (p = 0.007, hazard ratio 2.72, 95% CI 1.32 to 5.61). The expression of IL22 seemed to be microbiota dependent as broad-spectrum antibiotics significantly diminished IL22 expression (p = 0.019). Furthermore, IL22 expression significantly correlated with G-protein coupled receptor (GPR)43 (r = 0.263, p = 0.015) and GPR41 expression (r = 0.284, p = 0.009). In conclusion, our findings reveal an essential role of IL22 for the prognosis of patients undergoing allogeneic SCT.
Moving to the Outskirts: Interplay Between Regulatory T Cells and Peripheral Tissues
Authors: Estrada Brull Anna , Panetti Camilla , Joller Nicole
Published: April 2022

Regulatory T cells (Tregs) restrain excessive immune responses and dampen inflammation. In addition to this classical immune suppressive role, Tregs in non-lymphoid tissues also promote tissue homeostasis, regeneration and repair. In this review, we outline our current understanding of how Tregs migrate to peripheral tissues and the factors required for their maintenance at these sites. We discuss the tissue-specific adaptations of Tregs at barrier and immuno-privileged sites and the mechanisms that regulate their function within these organs. Furthermore, we outline what is known about the interactions of Tregs with non-immune cells in the different peripheral tissues at steady state and upon challenge or tissue damage. A thorough understanding of the tissue-specific adaptations and functions of Tregs will potentially pave the way for therapeutic approaches targeting their regenerative role.
Tissue Resident and Migratory Group 2 Innate Lymphoid Cells
Authors: Mathä Laura , Takei Fumio , Martinez-Gonzalez Itziar
Published: April 2022

Group 2 innate lymphoid cells (ILC2s) are present in both mouse and human mucosal and non-mucosal tissues and implicated in initiating type 2 inflammation. ILC2s are considered to be tissue resident cells that develop in the perinatal period and persist throughout life with minimal turning over in adulthood. However, recent studies in animal models have shown their ability to circulate between different organs during inflammation and their potential functions in the destined organs, suggesting their roles in mediating multiple type 2 diseases. Here, we review recent findings on ILC2 migration, including migration within, into and out of tissues during inflammation.
SARS-CoV-2 Omicron Variant: Epidemiological Features, Biological Characteristics, and Clinical Significance
Authors: Guo Yifei , Han Jiajia , Zhang Yao , He Jingjing , Yu Weien , Zhang Xueyun , Wu Jingwen , Zhang Shenyan , Kong Yide , Guo Yue , Lin Yanxue , Zhang Jiming
Published: April 2022

The SARS-CoV-2 Omicron (B.1.1529) variant was designated as a variant of concern (VOC) by the World Health Organization (WHO) on November 26, 2021. Within two months, it had replaced the Delta variant and had become the dominant circulating variant around the world. The Omicron variant possesses an unprecedented number of mutations, especially in the spike protein, which may be influencing its biological and clinical aspects. Preliminary studies have suggested that increased transmissibility and the reduced protective effects of neutralizing antibodies have contributed to the rapid spread of this variant, posing a significant challenge to control the coronavirus disease 2019 (COVID-19) pandemic. There is, however, a silver lining for this wave of the Omicron variant. A lower risk of hospitalization and mortality has been observed in prevailing countries. Booster vaccination also has ameliorated a significant reduction in neutralization. Antiviral drugs are minimally influenced. Moreover, the functions of Fc-mediated and T-cell immunity have been retained to a great extent, both of which play a key role in preventing severe disease.
NLRX1 Deficiency Alters the Gut Microbiome and Is Further Exacerbated by Adherence to a Gluten-Free Diet
Authors: Morrison Holly A. , Liu Yang , Eden Kristin , Nagai-Singer Margaret A. , Wade Paul A. , Allen Irving C.
Published: April 2022

Patients with gluten sensitivities present with dysbiosis of the gut microbiome that is further exacerbated by a strict adherence to a gluten-free diet (GFD). A subtype of patients genetically susceptible to gluten sensitivities are Celiac Disease (CeD) patients, who are carriers of the HLA DR3/DQ2 or HLA DR4/DQ8 haplotypes. Although 85-95% of all CeD patients carry HLA DQ2, up to 25-50% of the world population carry this haplotype with only a minority developing CeD. This suggests that CeD and other gluten sensitivities are mediated by factors beyond genetics. The contribution of innate immune system signaling has been generally understudied in the context of gluten sensitivities. Thus, here we examined the role of NOD-like receptors (NLRs), a subtype of pattern recognition receptors, in maintaining the composition of the gut microbiome in animals maintained on a GFD. Human transcriptomics data revealed significant increases in the gene expression of multiple NLR family members, across functional groups, in patients with active CeD compared to control specimens. However, NLRX1 was uniquely down-regulated during active disease. NLRX1 is a negative regulatory NLR that functions to suppress inflammatory signaling and has been postulate to prevent inflammation-induced dysbiosis. Using Nlrx1-/- mice maintained on either a normal or gluten-free diet, we show that loss of NLRX1 alters the microbiome composition, and a distinctive shift further ensues following adherence to a GFD, including a reciprocal loss of beneficial microbes and increase in opportunistic bacterial populations. Finally, we evaluated the functional impact of an altered gut microbiome by assessing short- and medium-chain fatty acid production. These studies revealed significant differences in a selection of metabolic markers that when paired with 16S rRNA sequencing data could reflect an overall imbalance and loss of immune system homeostasis in the gastrointestinal system.
Thioredoxin-1: A Promising Target for the Treatment of Allergic Diseases
Authors: Wang Jinquan , Zhou Jiedong , Wang Cuixue , Fukunaga Atsushi , Li Shujing , Yodoi Junji , Tian Hai
Published: April 2022

Thioredoxin-1 (Trx1) is an important regulator of cellular redox homeostasis that comprises a redox-active dithiol. Trx1 is induced in response to various stress conditions, such as oxidative damage, infection or inflammation, metabolic dysfunction, irradiation, and chemical exposure. It has shown excellent anti-inflammatory and immunomodulatory effects in the treatment of various human inflammatory disorders in animal models. This review focused on the protective roles and mechanisms of Trx1 in allergic diseases, such as allergic asthma, contact dermatitis, food allergies, allergic rhinitis, and drug allergies. Trx1 plays an important role in allergic diseases through processes, such as antioxidation, inhibiting macrophage migration inhibitory factor (MIF), regulating Th1/Th2 immune balance, modulating allergic inflammatory cells, and suppressing complement activation. The regulatory mechanism of Trx1 differs from that of glucocorticoids that regulates the inflammatory reactions associated with immune response suppression. Furthermore, Trx1 exerts a beneficial effect on glucocorticoid resistance of allergic inflammation by inhibiting the production and internalization of MIF. Our results suggest that Trx1 has the potential for future success in translational research.
The Yin and Yang of Targeting KLRG1+ Tregs and Effector Cells
Authors: Borys Samantha M. , Bag Arup K. , Brossay Laurent , Adeegbe Dennis O.
Published: April 2022

The literature surrounding KLRG1 has primarily focused on NK and CD8+T cells. However, there is evidence that the most suppressive Tregs express KLRG1. Until now, the role of KLRG1 on Tregs has been mostly overlooked and remains to be elucidated. Here we review the current literature on KLRG1 with an emphasis on the KLRG1+Treg subset role during cancer development and autoimmunity. KLRG1 has been recently proposed as a new checkpoint inhibitor target, but these studies focused on the effects of KLRG1 blockade on effector cells. We propose that when designing anti-tumor therapies targeting KLRG1, the effects on both effector cells and Tregs will have to be considered.
It’s the Little Things: The Role of Microscopic Life Stages in Maintaining Kelp Populations
Authors: Edwards Matthew S.
Published: April 2022

Kelp forests are experiencing broad scale declines in abundance and shifts in latitudinal ranges in many areas of the world due to numerous environmental stressors, especially those associated with climate change. While the majority of studies on kelp ecology have focused exclusively on the macroscopic sporophyte life stage, a growing number of studies is showing quite convincingly that the microscopic zoospore and gametophyte life stages can be important to establishing spatial patterns in these marine forests, and in facilitating their recovery following deforestation. Unfortunately, their microscopic sizes have made them difficult to study in the field, leading to a ‘black box’ surrounding what we know about their ecologies. However, advances in experimental methodologies and a growing number of laboratories studying kelp microscopic life stages are revealing how they are affected by variability in environmental conditions, and are providing a clearer picture of how kelp forests might respond to a changing ocean climate. These studies have largely revealed that kelps can disperse over a wide range of distances, enhanced by the synchronous release and vertical transport of zoospores into shallower water, and by floating rafts of reproductive thalli. Settlement on the benthos is facilitated by both positive and negative chemotaxis, and by active selection of microhabitats that favor their growth and survival. Following settlement and subsequent germination, the haploid gametophytes can delay their development and form a bank of microscopic forms that persist during periods that are unfavorable for the large sporophyte populations, or they can develop and undergo sexual reproduction during which they respond to variability in environmental conditions. In particular, they are strongly affected by increases in irradiance (PAR, UVA + UVB) and temperature, decreases in nutrients and salinity, and by sedimentation and grazing. However, the manner in which they respond to these stressors varies among species and with their geographic distributions, which is integral to establishing biogeographic patterns in the large sporophyte populations. Given these factors are expected to change as the ocean climate changes, these species-specific responses have significant implications for future patterns of distribution and abundance of these iconic marine forests.
Shear Mechanism of High-Strength-Friction-Grip Bolts in Steel and Steel-Fiber-Reinforced-Concrete Composite Beams
Authors: Zhang Yujie , Zhang Junping , Chen Bingcong , Zhang Yumin
Published: April 2022

Steel and steel-fiber-reinforced concrete (SFRC) composite beams with high-strength friction-grip bolt (HSFGB) connectors have been found to improve the shear behavior of HSFGBs and enhance the potential application of composite beams. In order to evaluate the shear force transmission mechanism of HSFGBs in steel-SFRC beams, finite element models (FEMs) developed by ABAQUS software had been carefully developed to evaluate force transmission and failure mechanisms of HSFGBs. Shear behavior differences between conventional studs and HSFGBs were also studied. Then according to the orthogonal statistics method, FEMs were further modified to explore significant influencing factors affecting HSFGB shear performance. The results showed that pretension degree was the main factor affecting the shear performance of HSFGBs at serviceability limit states, while HSFGB diameter was the most important factor affecting stiffness and ultimate shear strength at ultimate limit state.
Low-Osmolar vs. Iso-Osmolar Contrast Media on the Risk of Contrast-Induced Acute Kidney Injury: A Propensity Score Matched Study
Authors: Lee Taeho , Kim Won Ki , Kim Ae Jin , Ro Han , Chang Jae Hyun , Lee Hyun Hee , Chung Wookyung , Jung Ji Yong
Published: April 2022

Objective:

Among the various risk factors associated with contrast-induced acute kidney injury (CI-AKI), the importance of osmolality and viscosity is emerging among the characteristics of contrast media (CM) itself. High osmolality CM (HOCM) is deprecated and low osmotic pressure (LOCM) and iso-osmotic pressure (IOCM) are mainly used in clinical situations where the results of studies on their effect on the development of CI-AKI are contradictory. We evaluated the association between the type of CM and the risk of CI-AKI.

Materials and Methods

A retrospective observational cohort study to analyze the effect of the type of CM on the development of CI-AKI. Using propensity score (PS) matching, 2,263 LOCM and IOCM groups were paired for analysis from 5,267 patients and fulfilled the inclusion criteria among 12,742 patients who underwent CAG between 1 January 2007, and 31 December 2016. LOCM included iopromide and iopamidol, IOCM was iodixanol. CI-AKI, which was the primary endpoint, was defined based on the Kidney Disease Improving Global Outcomes criteria within 48 h after exposure to the CM. A multivariable logistic regression analysis was used in the unmatched and matched cohorts, respectively. In addition, a stratified model on clinically important variables, including a high Mehran score (≥ 6), was also used in the matched cohort.

Results

LOCM users showed an increased incidence of CI-AKI (11.7% vs. 9.3%; p = 0.006), but it lost statistical significance after PS matching (9.9% vs. 9.5%, p = 0.725). In multivariable analyses, the adjusted odds ratio for CI-AKI in the LOCM group were 1.059 [95% confidence interval (CI) = 0.875–1.282; p = 0.555] in unmatched cohort and 0.987 (95% CI = 0.803–1.214; p = 0.901) in matched cohort. These results were also consistent with the high-risk (high Mehran score) group.

Conclusions

Although the role of CM types in the development of CI-AKI has been debated, our observation shows that the selection between LOCM and IOCM during CAG has no influence on the incidence of CI-AKI.
Effects of Sodium Thiosulfate During Resuscitation From Trauma-and-Hemorrhage in Cystathionine-γ-Lyase Knockout Mice With Diabetes Type 1
Authors: Gröger Michael , Hogg Melanie , Abdelsalam Essam , Kress Sandra , Hoffmann Andrea , Stahl Bettina , Calzia Enrico , Wachter Ulrich , Vogt Josef A. , Wang Rui , Merz Tamara , Radermacher Peter , McCook Oscar
Published: April 2022

Background

Sodium thiosulfate (STS) is a recognized drug with antioxidant and H2S releasing properties. We recently showed that STS attenuated organ dysfunction and injury during resuscitation from trauma-and-hemorrhage in CSE-ko mice, confirming its previously described organ-protective and anti-inflammatory properties. The role of H2S in diabetes mellitus type 1 (DMT1) is controversial: genetic DMT1 impairs H2S biosynthesis, which has been referred to contribute to endothelial dysfunction and cardiomyopathy. In contrast, development and severity of hyperglycemia in streptozotocin(STZ)-induced DMT1 was attenuated in CSE-ko mice. Therefore, we tested the hypothesis whether STS would also exert organ-protective effects in CSE-ko mice with STZ-induced DMT1, similar to our findings in animals without underlying co-morbidity.

Methods

Under short-term anesthesia with sevoflurane and analgesia with buprenorphine CSE-ko mice underwent DMT1-induction by single STZ injection (100 μg⋅g–1). Seven days later, animals underwent blast wave-induced blunt chest trauma and surgical instrumentation followed by 1 h of hemorrhagic shock (MAP 35 ± 5 mmHg). Resuscitation comprised re-transfusion of shed blood, lung-protective mechanical ventilation, fluid resuscitation and continuous i.v. norepinephrine together with either i.v. STS (0.45 mg⋅g–1) or vehicle (n = 9 in each group). Lung mechanics, hemodynamics, gas exchange, acid–base status, stable isotope-based metabolism, and visceral organ function were assessed. Blood and organs were collected for analysis of cytokines, chemokines, and immunoblotting.

Results

Diabetes mellitus type 1 was associated with more severe circulatory shock when compared to our previous study using the same experimental design in CSE-ko mice without co-morbidity. STS did not exert any beneficial therapeutic effect. Most of the parameters measured of the inflammatory response nor the tissue expression of marker proteins of the stress response were affected either.

Conclusion

In contrast to our previous findings in CSE-ko mice without underlying co-morbidity, STS did not exert any beneficial therapeutic effect in mice with STZ-induced DMT1, possibly due to DMT1-related more severe circulatory shock. This result highlights the translational importance of both integrating standard ICU procedures and investigating underlying co-morbidity in animal models of shock research.
The Probiotic Strain Bifidobacterium animalis ssp. lactis HY8002 Potentially Improves the Mucosal Integrity of an Altered Intestinal Microbial Environment
Authors: Kim Joo Yun , Bang So-Jung , Kim Ju-Yeon , Choi Eun Jung , Heo Keon , Shim Jae-Jung , Lee Jung-Lyoul
Published: April 2022

Intestinal microbiota mediate the development and regulation of the intestinal immune system either directly or indirectly. Particularly, Bifidobacterium spp. play an important role in regulating the intestinal immunity and intestinal barrier. We demonstrated that Bifidobacterium animalis ssp. lactis HY8002, selected from eight Bifidobacterium strains by in vitro experimentation, had exceptional resistance to digestive tract conditions and high adhesion to intestinal epithelial cells and a positive effect on immunoglobulin A (IgA) secretion by Peyer’s patch cells. Moreover, HY8002 restored the expression of tight junction-related genes, initially reduced by lipopolysaccharide treatment, to normal levels in human intestinal epithelial cells. Notably, HY8002 restored kanamycin-induced reduction in Peyer’s patch cell numbers, serum and fecal IgA levels, and zonula occludens 1 and Toll-like receptor 2 levels in the mouse small intestine. In addition, HY8002 restores microbiome composition disturbed by kanamycin, and these microbiome changes have been found to correlate with TLR2 levels in the small intestine. Moreover, the ability of HY8002 to enhance IgA in Peyer’s patch cells and ZO-1 levels in intestinal epithelial cells was significantly inhibited by a TLR2 blocking antibody, which suggests that the HY8002 improve intestinal barrier function via TLR2. Finally, whole-genome sequencing of HY8002 revealed that it did not possess any known virulence factors. Therefore, HY8002 is a promising, functional probiotic supplement to improve intestinal barrier function by improving intestinal immunity and microbiota balance.
Phillygenin Inhibits Helicobacter pylori by Preventing Biofilm Formation and Inducing ATP Leakage
Authors: Li Ru-Jia , Qin Chun , Huang Gan-Rong , Liao Li-Juan , Mo Xiao-Qiang , Huang Yan-Qiang
Published: April 2022

With the widespread use and abuse of antibiotics, Helicobacter pylori (H. pylori) has become seriously drug resistant. The development of new antibiotics is an important way to solve H. pylori's drug resistance. Screening antibacterial ingredients from natural products is a convenient way to develop new antibiotics. Phillygenin, an effective antibacterial component, was selected from the natural product, forsythia, in this study. Its minimal inhibitory concentration (MIC) for 18 H. pylori strains was 16–32 μg/ml. The minimum bactericidal concentration (MBC) of H. pylori G27 was 128 μg/ml; the higher the drug concentration and the longer the time, the better the sterilization effect. It was non-toxic to gastric epithelial cell (GES)-1 and BGC823 cells at the concentration of 100 μg/ml. It presented a better antibacterial effect on H. pylori in an acidic environment, and after 24 days of induction on H. pylori with 1/4 MIC of phillygenin, no change was found in the MIC of H. pylori. In the mechanism of action, phillygenin could cause ATP leakage and inhibit the biofilm formation; the latter was associated with the regulation of spoT and Hp1174 genes. In addition, phillygenin could regulate the genes of Nhac, caggamma, MATE, MdoB, flagellinA, and lptB, leading to the weakening of H. pylori's acid resistance and virulence, the diminishing of H. pylori's capacity for drug efflux, H. pylori's DNA methylation, the initiation of human immune response, and the ATP leakage of H. pylori, thus accelerating the death of H. pylori. In conclusion, phillygenin was a main ingredient inhibiting H. pylori in Forsythia suspensa, with a good antibacterial activity, high safety, strong specificity, better antibacterial effect under acidic conditions, and low risk of resistance development by H. pylori. Its mechanism of action was mainly associated with inhibiting the biofilm formation and resulting in ATP leakage. In addition, phillygenin was shown to be able to reduce the acid resistance and virulence of H. pylori.
Metagenomic-Metabolomic Mining of Kinema, a Naturally Fermented Soybean Food of the Eastern Himalayas
Authors: Kharnaior Pynhunlang , Tamang Jyoti Prakash
Published: April 2022

Kinema is a popular sticky fermented soybean food of the Eastern Himalayan regions of North East India, east Nepal, and south Bhutan. We hypothesized that some dominant bacteria in kinema may contribute to the formation of targeted and non-targeted metabolites for health benefits; hence, we studied the microbiome–metabolite mining of kinema. A total of 1,394,094,912 bp with an average of 464,698,304 ± 120,720,392 bp was generated from kinema metagenome, which resulted in the identification of 47 phyla, 331 families, 709 genera, and 1,560 species. Bacteria (97.78%) were the most abundant domain with the remaining domains of viruses, eukaryote, and archaea. Firmicutes (93.36%) was the most abundant phylum with 280 species of Bacillus, among which Bacillus subtilis was the most dominant species in kinema followed by B. glycinifermentans, B. cereus, B. licheniformis, B. thermoamylovorans, B. coagulans, B. circulans, B. paralicheniformis, and Brevibacillus borstelensis. Predictive metabolic pathways revealed the abundance of genes associated with metabolism (60.66%), resulting in 216 sub-pathways. A total of 361 metabolites were identified by metabolomic analysis (liquid chromatography-mass spectrophotometry, LC-MS). The presence of metabolites, such as chrysin, swainsonine, and 3-hydroxy-L-kynurenine (anticancer activity) and benzimidazole (antimicrobial, anticancer, and anti-HIV activities), and compounds with immunomodulatory effects in kinema supports its therapeutic potential. The correlation between the abundant species of Bacillus and primary and secondary metabolites was constructed with a bivariate result. This study proves that Bacillus spp. contribute to the formation of many targeted and untargeted metabolites in kinema for health-promoting benefits.
Adaptive Pathways of Microorganisms to Cope With the Shift From P- to N-Limitation in Subtropical Plantations
Authors: Wang Chaoqun , Jiao Ruzhen
Published: April 2022

Ecological stoichiometry is increasingly acknowledged as one of the main control factors for microbial activity and diversity. Soil carbon/nitrogen (C/N) and carbon/phosphorus (C/P) ratios are usually much higher than microbial nutrient requirements and vary with planting density and stand age in forestlands. However, how microorganisms cope with such stoichiometric imbalances and how they regulate nutrient cycling remain unclear. Here, 5- and 35-year-old experimentalCunninghamia lanceolata[Lamb.] Hook plantations with five planting densities (1,667, 3,333, 5,000, 6,667, and 10,000 stems ha−1) were used to explore the underlying mechanism of the response of microorganisms to stoichiometric imbalances. We found that (i) enzyme activity and microbial biomass and diversity increased with planting density at age 5 but decreased at age 35; (ii) soil microorganisms were P-limited at age 5, but gradually shifted from P- to N-limitation during the development of plantations from 5 to 35 years; and (iii) significantly negative relationships between microbial biomass stoichiometry and enzymatic stoichiometry were observed. The adaptive pathways of soil microorganisms to cope with stoichiometric imbalances include (i) adjusting the stoichiometry of microorganisms and enzymes; (ii) changing the relative abundance of the dominant microbial phyla; and (iii) increasing the ratio of fungal to bacterial diversity. These results highlight how to use the ecological stoichiometry method to identify soil microbial nutrient limitations with planting density during the development of plantations. By underlining the important role of stoichiometry on microbial growth and activity, these findings furthermore emphasize the dependency of organic matter transformation and nutrient cycling on the microbial community.