bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Face Masks on Physiological and Perceptual Responses during 30 Minutes of Self-Paced Exercise in Older Community Dwelling Adults
Authors: Vogt Grace , Radtke Kimberley , Jagim Andrew , Peckumn Dominique , Lee Teresa , Mikat Richard , Foster Carl
Published: October 2022

This study examined the effects of different types of masks (no mask, surgical mask (SM), and N95-mask) on physiological and perceptual responses during 30-min of self-paced cycle ergometer exercise. This study was a prospective randomly assigned experimental design. Outcomes included workload (Watts), oxygen saturation (SpO2), end-tidal carbon dioxide (PetCO2), heart rate (HR), respiratory rate (RR), rating of perceived exertion (RPE), and rating of perceived dyspnea (RPD). Volunteers (54–83 years (n = 19)) completed two familiarization sessions and three testing sessions on an air braked cycle ergometer. No significant difference was found for condition x time for any of the dependent variables. RPE, RPD, and PetCO2 were significantly higher with an N95-mask vs. no mask (NM) ((p = 0.012), (p = 0.002), (p < 0.001)). HR was significantly higher with the SM compared to the NM condition (p = 0.027) (NM 107.18 ± 9.96) (SM 112.34 ± 10.28), but no significant difference was found when comparing the SM to the N95 condition or when comparing the N95condition to the NM condition. Watts increased across time in each condition (p = 0.003). Initially RR increased during the first 3 min of exercise (p < 0.001) with an overall gradual increase noted across time regardless of mask condition (p < 0.001). SpO2 significantly decreased across time but remained within normal limits (>95%). No significant difference was found in Watts, RR, or SpO2 regardless of mask condition. Overall, the N95mask was associated with increased RPE, RPD, and PetCO2 levels. This suggests trapping of CO2 inside the mask leading to increased RPE and RPD.
Families, Schools, and the Longitudinal Changes in Psychological Distress among College Students during the First Year of the COVID-19 Pandemic: Evidence from a National Panel Survey in China
Authors: Zhu Fengxia , Zhang Yueyun , Li Qi , Xu Yuanyao , Liu Baozhong
Published: October 2022

Background: The psychological well-being of students in higher education has become an increasingly important concern in the context of the COVID-19 pandemic. The majority of prior studies were cross-sectional and thereby failed to capture the individual changes in mental health over time. Moreover, few studies have explored whether and how socioeconomic and education backgrounds could be related to college students’ mental health changes. This study aimed to fill these gaps. Methods: Data were from a nationwide, two-wave panel survey of college students in China. Baseline information was collected in November 2019, i.e., before the outbreak of the COVID-19 epidemic in China. A follow-up survey was conducted in November and December 2020, when the epidemic was effectively contained in mainland China. In both waves, mental health was assessed with the 10-item Kessler Psychological Distress Scale (K10). The between-wave changes in psychological distress were measured and categorized. Results: Overall, 13.5% of students experienced at least a one-standard-deviation increase in psychological distress over the one-year follow-up. Results from both bivariate and multivariable analysis showed that this marked distress increase was more pronounced among vocational college students (vs. academic undergraduate and postgraduate students) and those with lower levels of academic performance. In contrast, family socioeconomic status (as measured by parental education, family economic condition, and current residence) was not associated with distress changes over time. Conclusions: These findings highlight the importance of the educational disparities within the higher education system in understanding the mental health dynamics of college students in the context of the COVID-19 pandemic.
Spatiotemporal Variations of Carbon Emissions and Their Driving Factors in the Yellow River Basin
Authors: Wang Shiqing , Sun Piling , Sun Huiying , Liu Qingguo , Liu Shuo , Lu Da
Published: October 2022

The Yellow River Basin (YRB) is a significant area of economic development and ecological protection in China. Scientifically clarifying the spatiotemporal patterns of carbon emissions and their driving factors is of great significance. Using the methods of spatial autocorrelation analysis, hot-spot analysis, and a geodetector, the analysis framework of spatiotemporal differentiation and the driving factors of carbon emissions in the YRB was constructed in this paper from three aspects: natural environment, social economy, and regional policy. Three main results were found: (1) The carbon emissions in the YRB increased gradually from 2000 to 2020, and the growth rates of carbon emissions in the different river reaches were upper reaches > middle reaches > lower reaches. (2) Carbon emissions have an obvious spatial clustering character from 2000–2020, when hot spots were concentrated in the transition area from the Inner Mongolia Plateau to the Loess Plateau. The cold spots of carbon emissions tended to be concentrated in the junction area of Qinghai, Gansu, and Shaanxi. (3) From 2000 to 2020, the driving factors of spatial differentiation of carbon emissions in the YRB and its different reaches tended to be diversified, so the impacts of socioeconomic factors increased, while the impacts of natural environmental factors decreased. The influence of the interactions of each driving factor showed double factor enhancement and nonlinear enhancement. This study will provide a scientific reference for green and low-carbon development, emphasizing the need to pay more attention to environmental protection, develop the green economy vigorously, and promote the economic cycle, so as to achieve green development and reduce carbon emissions.
Victimization of Applicants for International Protection Residing in Belgium: Sexual Violence and Help-Seeking Behavior
Authors: De Schrijver Lotte , Nobels Anne , Harb Jonathan , Nisen Laurent , Roelens Kristien , Vander Beken Tom , Vandeviver Christophe , Keygnaert Ines
Published: October 2022

Background: Sexual violence (SV) literature on applicants for international protection (AIPs) shows that they are at high risk of victimization. The study objectives are to provide an exploratory overview of the occurrence of SV in AIPs in Belgium and their help-seeking behavior (HSB). This overview is crucial to develop prevention strategies and care paths focusing on providing adequate care to AIP SV victims in Belgium. Methods: Quantitative data from structured interviews with AIPs (n = 62) triangulated with qualitative data from in-depth interviews with AIP SV victims (n = 11) served to explore the nature and impact of SV in AIPs in Belgium and their HSB. Results: A total of 83.9% of respondents have experienced SV. A total of 61.3% were victimized within the past year. Victimization seems more gender-balanced than in the general population. AIPs link SV to their legal status and their associated vulnerable situation. HSB upon SV was very limited in this sample. Help-seeking barriers interfering with the decision-making process to consult (in)formal resources were identified. Conclusions: AIPs in Belgium are at high risk of SV. Despite the impact of SV on AIPs’ lives, HSB upon SV is rare. The provision of age-appropriate sexual education and development of policies that will reduce help-seeking barriers is needed.
COVID-19 Vaccination Acceptance in the Context of the Health Belief Model: Comparative Cross-Sectional Study in Punjab, Pakistan
Authors: Zakar Rubeena , Momina Ain ul , Shahzad Ruhma , Shahzad Sara , Hayee Mahwish , Zakar Muhammad Zakria , Fischer Florian
Published: October 2022

One of the models that could be used to understand the adoption of vaccine uptake is the Health Belief Model (HBM). The aim of this study is to assess the role of HBM constructs and Perceived Health Status (PHS) on the vaccination status of individuals and to understand the role of socio-demographic variables on HBM scoring. A comparative cross-sectional telephone survey was conducted among 1325 vaccinated (60.0%) and non-vaccinated (40.0%) individuals aged 40 years and above in July 2021 in Punjab province, Pakistan. A higher level of education was the strongest predictor of positive HBM. All constructs of HBM, PHS and cues-to-action were significant predictors of COVID-19 vaccination uptake, with perceived benefits as the strongest predictor. In order to expand the vaccination coverage, double-pronged interventions utilizing both information and communication technology and human resources should be designed that address each barrier perceived by individuals and understandably communicate the benefits of COVID-19 vaccination to the broader population.
Alveolar Type II Cell Damage and Nrf2-SOD1 Pathway Downregulation Are Involved in PM2.5-Induced Lung Injury in Rats
Authors: Niu Rui , Cheng Jie , Sun Jian , Li Fan , Fang Huanle , Lei Ronghui , Shen Zhenxing , Hu Hao , Li Jianjun
Published: October 2022

The general toxicity of fine particulate matter (PM2.5) has been intensively studied, but its pulmonary toxicities are still not fully understood. To investigate the changes of lung tissue after PM2.5 exposure and identify the potential mechanisms of pulmonary toxicity, PM2.5 samples were firstly collected and analyzed. Next, different doses of PM2.5 samples (5 mg/kg, 10 mg/kg, 20 mg/kg) were intratracheally instilled into rats to simulate lung inhalation of polluted air. After instillation for eight weeks, morphological alterations of the lung were examined, and the levels of oxidative stress were detected. The data indicated that the major contributors to PM2.5 mass were organic carbon, elemental carbon, sulfate, nitrate, and ammonium. Different concentrations of PM2.5 could trigger oxidative stress through increasing reactive oxygen species (ROS) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels, and decreasing expression of antioxidant-related proteins (nuclear factor erythroid 2-related factor 2 (Nrf2), superoxide dismutase 1 (SOD1) and catalase). Histochemical staining and transmission electron microscopy displayed pulmonary inflammation, collagen deposition, mitochondrial swelling, and a decreasing number of multilamellar bodies in alveolar type II cells after PM2.5 exposure, which was related to PM2.5-induced oxidative stress. These results provide a basis for a better understanding of pulmonary impairment in response to PM2.5.
Influencing Factors on Airflow and Pollutant Dispersion around Buildings under the Combined Effect of Wind and Buoyancy—A Review
Authors: Wu Mei , Zhang Guangwei , Wang Liping , Liu Xiaoping , Wu Zhengwei
Published: October 2022

With the rapid growth of populations worldwide, air quality has become an increasingly important issue related to the health and safety of city inhabitants. There are quite a few factors that contribute to urban air pollution; the majority of studies examining the issue are concerned with environmental conditions, building geometries, source characteristics and other factors and have used a variety of approaches, from theoretical modelling to experimental measurements and numerical simulations. Among the environmental conditions, solar-radiation-induced buoyancy plays an important role in realistic conditions. The thermal conditions of the ground and building façades directly affect the wind field and pollutant dispersion patterns in the microclimate. The coupling effect of wind and buoyancy on the urban environment are currently hot and attractive research topics. Extensive studies have been devoted to this field, some focused on the street canyon scale, and have found that thermal effects do not significantly affect the main airflow structure in the interior of the street canyon but strongly affect the wind velocity and pollutant concentration at the pedestrian level. Others revealed that the pollutant dispersion routes can be obviously different under various Richardson numbers at the scale of the isolated building. The purpose of this review is therefore to systematically articulate the approaches and research outcomes under the combined effect of wind and buoyancy from the street canyon scale to an isolated building, which should provide some insights into future modelling directions in environmental studies.
Changes in Air Quality and Drivers for the Heavy PM2.5 Pollution on the North China Plain Pre- to Post-COVID-19
Authors: Liu Shuang , Yang Xingchuan , Duan Fuzhou , Zhao Wenji
Published: October 2022

Under the clean air action plans and the lockdown to constrain the coronavirus disease 2019 (COVID-19), the air quality improved significantly. However, fine particulate matter (PM2.5) pollution still occurred on the North China Plain (NCP). This study analyzed the variations of PM2.5, nitrogen dioxide (NO2), sulfur dioxide (SO2), carbon monoxide (CO), and ozone (O3) during 2017–2021 on the northern (Beijing) and southern (Henan) edges of the NCP. Furthermore, the drivers for the PM2.5 pollution episodes pre- to post-COVID-19 in Beijing and Henan were explored by combining air pollutant and meteorological datasets and the weighted potential source contribution function. Results showed air quality generally improved during 2017–2021, except for a slight rebound (3.6%) in NO2 concentration in 2021 in Beijing. Notably, the O3 concentration began to decrease significantly in 2020. The COVID-19 lockdown resulted in a sharp drop in the concentrations of PM2.5, NO2, SO2, and CO in February of 2020, but PM2.5 and CO in Beijing exhibited a delayed decrease in March. For Beijing, the PM2.5 pollution was driven by the initial regional transport and later secondary formation under adverse meteorology. For Henan, the PM2.5 pollution was driven by the primary emissions under the persistent high humidity and stable atmospheric conditions, superimposing small-scale regional transport. Low wind speed, shallow boundary layer, and high humidity are major drivers of heavy PM2.5 pollution. These results provide an important reference for setting mitigation measures not only for the NCP but for the entire world.
Former Incarceration, Time Served, and Perceived Oral Health among African American Women and Men
Authors: Talbert Ryan D. , Macy Emma D.
Published: October 2022

A large body of research has documented the far-reaching health consequences of mass incarceration in the United States. Yet, less scholarship has examined the relationship between former incarceration and oral health, a key reflection of health and disease occurring within the rest of the body. Using data extracted from the National Survey of American Life (n = 3343), this study examines associations among former incarceration status, duration of detention, and self-reported oral health among African American women and men. Results from gender-stratified ordered logistic models reveal that formerly incarcerated African American men and women experience significantly poorer oral health than their never incarcerated counterparts even after controlling for important social determinants of health. Furthermore, oral health is curvilinearly associated with the length of time that men are incarcerated such that odds of poor health decrease as detention duration increases up to approximately 15 years incarcerated. After 15 years of detainment, the odds of poor health tend to increase as duration increases. Findings extend research identifying gendered spillover health consequences of contact with the criminal legal system. Health professionals and policymakers should be conscious of incarceration as an important deleterious experience for the immediate and long-term condition of people’s teeth, mouth, and gums.
Exploring the Experiences of Runners with Visual Impairments and Sighted Guides
Authors: Ball Lindsay E. , Lieberman Lauren J. , Beach Pamela , Perreault Melanie , Rich Jason
Published: October 2022

Running is a popular sport, and, with simple modifications, it can be accessible for individuals with visual impairments, particularly with a sighted running guide. The purpose of this study is to examine the experiences of runners with visual impairments and sighted running guides. Adopting a descriptive qualitative approach to guide data collection analysis and interpretation, seven runners with visual impairments and four sighted running guides were recruited and interviewed. The analysis identified four major themes: (1) benefits, (2) barriers, (3) advocacy, and (4) communication. The identified themes illustrate the influence of participation in running on the health and relationships of the runners with visual impairments, the barriers that exist to participation, and the advocacy and communication needed to overcome those barriers.
Examining the Factors Affecting the Adoption of Blockchain Technology in the Banking Sector: An Extended UTAUT Model
Authors: Jena Rabindra Kumar
Published: October 2022

Technology innovation has dramatically transformed banks over time. Digital innovation in the banking sector began with the introduction of money to replace barter systems, and then gradually replaced wax seals with digital signatures. One such disruptive innovation that is transforming the banking sector around the world is blockchain technology (BCT). The banking sector in India has also started adopting blockchain technology in various financial transactions. However, they are encountering some difficulties in adapting to and implementing this new technology. The successful and speedy adoption of blockchain in banking largely depends on the users’ intention to use the services. Therefore, this study extended “the unified theory of acceptance and use of technology” (UTAUT) to understand the significant predictors of the bankers’ intention to use blockchain technology. The data was collected from leading banking institutions and FinTech firms in the country to empirically test and validate the extended model. The results found that facilitating conditions, performance expectancy, and initial trust, are the significant antecedents to predicting the bankers’ intention to use blockchain in banking transactions. The study also established the significant mediating role of initial trust in predicting usage intention to use blockchain. This study’s results would help government authorities, decision-makers, and technocrats to improve banking instructions for the speedy and smooth adoption of blockchain technology. The study suggested an extended UTAUT model that incorporates contextual factors based on the scope and usage of blockchain in Indian banking activities. The study helped to identify the key factors influencing blockchain adoption among Indian bankers. The proposed model and the findings make more sense in promoting the adoption of blockchain in the Indian banking sector.
The Role of Motivated Financial Institutions on Community Currencies Loans
Authors: de Oliveira Leite Rodrigo , dos Santos Mendes Layla , Tommasetti Roberto , Maia Vinicius Mothe , Larrain Rodrigo Soto
Published: October 2022

Community currencies have recently emerged as tools for assisting the disadvantaged. In order to make a contribution to the larger field of community currencies, the purpose of this research is to investigate how a financial institution evaluates community currency lending. We show, using an adverse selection model, the importance of the role of motivated financial institutions in the effectiveness of community currency loans for small entrepreneurs. If those institutions are unmotivated, the market collapses, and only traditional loans are offered. Additionally, if there is enough intrinsic motivation, the size of the loans also increases, which is beneficial to the borrowers. Finally, this paper emphasizes the importance of subsidies in this sector, which act as catalysts by increasing the likelihood that community currency loans will be offered, as well as increasing loan sizes.
Passenger Flow Prediction of Scenic Spots in Jilin Province Based on Convolutional Neural Network and Improved Quantile Regression Long Short-Term Memory Network
Authors: Qin Xiwen , Yin Dongmei , Dong Xiaogang , Chen Dongxue , Zhang Shuang
Published: October 2022

Passenger flow is an important benchmark for measuring tourism benefits, and accurate tourism passenger flow prediction is of great significance to the government and related tourism enterprises and can promote the sustainable development of China’s tourism industry. For daily passenger flow time series data, a passenger flow forecasting method based on convolutional neural network (CNN) and improved quantile regression long short-term memory network (QRLSTM), denoted as CNN-IQRLSTM, is proposed with reconstructed correlation features and in the form of sliding windows as inputs. First, four discrete variables such as whether the day is a weekend and holiday are created by time; then, a sliding window of width 42 is used to pass the passenger flow data into the network sequentially; finally, the loss function of the sparse Laplacian improved QRLSTM is introduced for passenger flow prediction, and the point prediction and interval prediction results under different quartiles are obtained. The application of quantile regression captures the overall picture of the data, enhances the robustness, fit, predictive power and nonlinear processing capability of neural networks, and fills the gap between quantile regression and neural network methods in the field of passenger flow prediction. CNN can effectively handle complex input data, and the improved nonlinear QR model can provide passenger flow quantile prediction information. The method is applied to the tourism traffic prediction of four 5A scenic spots in Jilin Province, and the effectiveness of the method is verified. The results show that the method proposed in this paper fits best in point prediction and has higher prediction accuracy. The MAPE of the Changbai Mountain dataset was 0.07, the MAPE of the puppet palace museum dataset was 0.05, the fit of the Sculpture Park dataset reached 93%, and the fit of the net moon lake dataset was as high as 99%. Meanwhile, the interval prediction results show that the method has a larger interval coverage as well as a smaller interval average width, which improves the prediction efficiency. In 95% of the interval predictions, the interval coverage of Changbai Mountain data is 99% and the interval average width is 0.49. It is a good reference value for the management of different scenic spots.
Analysis of the Evolution of the Relationship between the Urban Pattern and Economic Development in Guangdong Province Based on Coupled Multisource Data
Authors: Li Pengfei , Hao Shuang , Cui Yuhuan , Xu Yazhou , Liao Congcong , Sheng Liangliang
Published: October 2022

Regarding the rapid development of urban agglomeration (UA) in Guangdong Province in the past two decades, this study revealed the temporal and spatial evolution of the urban pattern of the province and the current urbanization process. This study determined the geographical spatial distribution and change in the UA lighting scale in Guangdong Province, analyzed the relationship between the lighting change and development and the dynamic evolution of the gross domestic product, and explored the expansion intensity and center of gravity migration direction of UA. The results showed that from 2000 to 2020, the lighting scale of the border areas of Guangdong Province was lower than that of the inland areas, whereas the lighting growth rate of the border areas was higher than that of the inland areas. The built-up area steadily expanded from the center to the outside within the time range of the study, and the center of gravity of the ellipse tended to shift northwest. The study provides visual and scientific data for the spatiotemporal evolution of the urban pattern in Guangdong Province and has important reference significance for analyzing urbanization development and planning urban construction.
Genome-Wide Identification of the A20/AN1 Zinc Finger Protein Family Genes in Ipomoea batatas and Its Two Relatives and Function Analysis of IbSAP16 in Salinity Tolerance
Authors: Xie Hao , Yang Qiangqiang , Wang Xiaoxiao , Schläppi Michael R. , Yan Hui , Kou Meng , Tang Wei , Wang Xin , Zhang Yungang , Li Qiang , Dai Shaojun , Liu Yaju
Published: October 2022

Stress-associated protein (SAP) genes—encoding A20/AN1 zinc-finger domain-containing proteins—play pivotal roles in regulating stress responses, growth, and development in plants. They are considered suitable candidates to improve abiotic stress tolerance in plants. However, the SAP gene family in sweetpotato (Ipomoea batatas) and its relatives is yet to be investigated. In this study, 20 SAPs in sweetpotato, and 23 and 26 SAPs in its wild diploid relatives Ipomoea triloba and Ipomoea trifida were identified. The chromosome locations, gene structures, protein physiological properties, conserved domains, and phylogenetic relationships of these SAPs were analyzed systematically. Binding motif analysis of IbSAPs indicated that hormone and stress responsive cis-acting elements were distributed in their promoters. RT-qPCR or RNA-seq data revealed that the expression patterns of IbSAP, ItbSAP, and ItfSAP genes varied in different organs and responded to salinity, drought, or ABA (abscisic acid) treatments differently. Moreover, we found that IbSAP16 driven by the 35 S promoter conferred salinity tolerance in transgenic Arabidopsis. These results provided a genome-wide characterization of SAP genes in sweetpotato and its two relatives and suggested that IbSAP16 is involved in salinity stress responses. Our research laid the groundwork for studying SAP-mediated stress response mechanisms in sweetpotato.
The Giardial Arginine Deiminase Participates in Giardia-Host Immunomodulation in a Structure-Dependent Fashion via Toll-like Receptors
Authors: Fernández-Lainez Cynthia , de la Mora-de la Mora Ignacio , Enríquez-Flores Sergio , García-Torres Itzhel , Flores-López Luis A. , Gutiérrez-Castrellón Pedro , de Vos Paul , López-Velázquez Gabriel
Published: October 2022

Beyond the problem in public health that protist-generated diseases represent, understanding the variety of mechanisms used by these parasites to interact with the human immune system is of biological and medical relevance. Giardia lamblia is an early divergent eukaryotic microorganism showing remarkable pathogenic strategies for evading the immune system of vertebrates. Among various multifunctional proteins in Giardia, arginine deiminase is considered an enzyme that plays multiple regulatory roles during the life cycle of this parasite. One of its most important roles is the crosstalk between the parasite and host. Such a molecular “chat” is mediated in human cells by membrane receptors called Toll-like receptors (TLRs). Here, we studied the importance of the 3D structure of giardial arginine deiminase (GlADI) to immunomodulate the human immune response through TLRs. We demonstrated the direct effect of GlADI on human TLR signaling. We predicted its mode of interaction with TLRs two and four by using the AlphaFold-predicted structure of GlADI and molecular docking. Furthermore, we showed that the immunomodulatory capacity of this virulent factor of Giardia depends on the maintenance of its 3D structure. Finally, we also showed the influence of this enzyme to exert specific responses on infant-like dendritic cells.
Naked and Decorated Nanoparticles Containing H2S-Releasing Doxorubicin: Preparation, Characterization and Assessment of Their Antitumoral Efficiency on Various Resistant Tumor Cells
Authors: Peira Elena , Chirio Daniela , Sapino Simona , Chegaev Konstantin , Chindamo Giulia , Salaroglio Iris Chiara , Riganti Chiara , Gallarate Marina
Published: October 2022

Several semisynthetic, low-cardiotoxicity doxorubicin (DOXO) conjugated have been extensively described, considering the risk of cytotoxicity loss against resistant tumor cells, which mainly present drug efflux capacity. Doxorubicin 14-[4-(4-phenyl-5-thioxo-5H-[1,2]dithiol-3-yl)]-benzoate (H2S-DOXO) was synthetized and tested for its ability to overcome drug resistance with good intracellular accumulation. In this paper, we present a formulation study aimed to develop naked and decorated H2S-DOXO-loaded lipid nanoparticles (NPs). NPs prepared by the “cold dilution of microemulsion” method were decorated with hyaluronic acid (HA) to obtain active targeting and characterized for their physicochemical properties, drug entrapment efficiency, long-term stability, and in vitro drug release. Best formulations were tested in vitro on human-sensitive (MCF7) and human/mouse DOXO-resistant (MDA-MDB -231 and JC) breast cancer cells, on human (U-2OS) osteosarcoma cells and DOXO-resistant human/mouse osteosarcoma cells (U-2OS/DX580/K7M2). HA-decoration by HA-cetyltrimethyl ammonium bromide electrostatic interaction on NPs surface was confirmed by Zeta potential and elemental analysis at TEM. NPs had mean diameters lower than 300 nm, 70% H2S-DOXO entrapment efficiency, and were stable for almost 28 days. HA-decorated NPs accumulated H2S-DOXO in Pgp-expressing cells reducing cell viability. HA-decorated NPs result in the best formulation to increase the inter-cellular H2S-DOXO delivery and kill resistant cells, and therefore, as a future perspective, they will be taken into account for further in vivo experiments on tumor animal model.
Embryogenic Stem Cell Identity after Protoplast Isolation from Daucus carota and Recovery of Regeneration Ability through Protoplast Culture
Authors: Han Jong-Eun , Lee Han-Sol , Lee Hyoshin , Cho Hyunwoo , Park So-Young
Published: October 2022

Protoplasts are single cells isolated from tissues or organs and are considered a suitable system for cell studies in plants. Embryogenic cells are totipotent stem cells, but their regeneration ability decreases or becomes lost altogether with extension of the culture period. In this study, we isolated and cultured EC-derived protoplasts (EC-pts) from carrots and compared them with non-EC-derived protoplasts (NEC-pts) with respect to their totipotency. The protoplast isolation conditions were optimized, and the EC-pts and NEC-pts were characterized by their cell size and types. Both types of protoplasts were then embedded using the alginate layer (TAL) method, and the resulting EC-pt-TALs and NEC-pt-TALs were cultured for further regeneration. The expression of the EC-specific genes SERK1, WUS, BBM, LEC1, and DRN was analyzed to confirm whether EC identity was maintained after protoplast isolation. The protoplast isolation efficiency for EC-pts was 2.4-fold higher than for NEC-pts (3.5 × 106 protoplasts·g−1 FW). In the EC-pt group, protoplasts < 20 µm accounted for 58% of the total protoplasts, whereas in the NEC-pt group, small protoplasts accounted for only 26%. In protoplast culture, the number of protoplasts that divided was 2.6-fold higher for EC-pts than for NEC-pts (7.7 × 104 protoplasts·g−1 FW), with a high number of plants regenerated for EC-pt-TALs, whereas no plants were induced by NEC-pt-TAL. Five times more plants were regenerated from EC-pts than from ECs. Regarding the expression of EC-specific genes, WUS and SERK1 expression increased 12-fold, and LEC1 and BBM expression increased 3.6–6.4-fold in isolated protoplasts compared with ECs prior to protoplast isolation (control). These results reveal that the protoplast isolation process did not affect the embryogenic cell identity; rather, it increased the plant regeneration rate, confirming that EC-derived protoplast culture may be an efficient system for increasing the regeneration ability of old EC cultures through the elimination of old and inactivate cells. EC-derived protoplasts may also represent an efficient single-cell system for application in new breeding technologies such as genome editing.
Gene Signatures Associated with Temporal Rhythm as Diagnostic Markers of Major Depressive Disorder and Their Role in Immune Infiltration
Authors: Wang Jing , Ai Pan , Sun Yi , Shi Hui , Wu Anshi , Wei Changwei
Published: October 2022

Temporal rhythm (TR) is involved in the pathophysiology and treatment response of major depressive disorder (MDD). However, there have been few systematic studies on the relationship between TR-related genes (TRRGs) and MDD. This study aimed to develop a novel prognostic gene signature based on the TRRGs in MDD. We extracted expression information from the Gene Expression Omnibus (GEO) database and retrieved TRRGs from GeneCards. Expressed genes (TRRDEGs) were identified differentially, and their potential biological functions were analyzed. Subsequently, association analysis and receiver operating characteristic (ROC) curves were adopted for the TRRDEGs. Further, upstream transcription factor (TF)/miRNA and potential drugs targeting MDD were predicted. Finally, the CIBERSORT algorithm was used to estimate the proportions of immune cell subsets. We identified six TRRDEGs that were primarily involved in malaria, cardiac muscle contraction, and the calcium-signaling pathway. Four genes (CHGA, CCDC47, ACKR1, and FKBP11) with an AUC of >0.70 were considered TRRDEGs hub genes for ROC curve analysis. Outcomes showed that there were a higher ratio of T cells, gamma-delta T cells, monocytes, and neutrophils, and lower degrees of CD8+ T cells, and memory resting CD4+ T cells in TRRDEGs. Four new TRRDEG signatures with excellent diagnostic performance and a relationship with the immune microenvironment were identified.
Tumor-Intrinsic Nuclear β-Catenin Associates with an Immune Ignorance Phenotype and a Poorer Prognosis in Head and Neck Squamous Cell Carcinomas
Authors: Sánchez-Canteli Mario , Juesas Luis , Garmendia Irati , Otero-Rosales María , Calvo Alfonso , Alvarez-Fernández Monica , Astudillo Aurora , Montuenga Luis M. , García-Pedrero Juana M. , Rodrigo Juan P.
Published: October 2022

Activation of WNT/β-catenin signaling has been associated with a non-T-cell-inflamed tumor microenvironment (TME) in several cancers. The aim of this work was to investigate the relationship between β-catenin signaling and TME inflammation in head and neck squamous cell carcinomas (HNSCCs). Membrane and nuclear β-catenin expression, PD-L1 expression, and CD8+ tumor-infiltrating lymphocyte (TIL) density were jointly evaluated by immunohistochemistry in a series of 372 HPV-negative HNSCCs. Membrane β-catenin levels decreased in carcinomas compared to the normal epithelium. Positive nuclear β-catenin was detected in 50 tumors (14.3%) and was significantly associated with a low CD8+ TIL density (168 cells/mm2 versus 293 cells/mm2 in nuclear-β-catenin-negative cases; p = 0.01) and a tendency for a lower expression of PD-L1, resulting in association with a noninflamed TME (i.e., type II, immunological ignorance). Multivariate Cox analysis further demonstrated that low infiltration by CD8+ TILs (HR = 1.6, 95% CI = 1.19–2.14, p = 0.002) and nuclear β-catenin expression (HR = 1.47, 95% CI = 1.01–2.16, p = 0.04) were both independently associated with a poorer disease-specific survival. In conclusion, tumor-intrinsic nuclear β-catenin activation is associated with a non-inflamed TME phenotype and a poorer prognosis, thereby suggesting a possible implication as an immune exclusion mechanism for a subset of HNSCC patients.
A Pathophysiological Intersection of Diabetes and Alzheimer’s Disease
Authors: Čater Maša , Hölter Sabine M.
Published: October 2022

Diabetes is among the most prevalent diseases of the modern world and is strongly linked to an increased risk of numerous neurodegenerative disorders, although the exact pathophysiological mechanisms are not clear yet. Insulin resistance is a serious pathological condition, connecting type 2 diabetes, metabolic syndrome, and obesity. Recently, insulin resistance has been proven to be connected also to cognitive decline and dementias, including the most prevalent form, Alzheimer’s disease. The relationship between diabetes and Alzheimer’s disease regarding pathophysiology is so significant that it has been proposed that some presentations of the condition could be termed type 3 diabetes.
Over-Expression of Phosphoserine Aminotransferase-Encoding Gene (AtPSAT1) Prompts Starch Accumulation in L. turionifera under Nitrogen Starvation
Authors: Wang Lei , Li Shuiling , Sun Ling , Tong Yana , Yang Lin , Zhu Yerong , Wang Yong
Published: October 2022

It has been demonstrated that the phosphorylation pathway of L-serine (Ser) biosynthesis (PPSB) is very important in plant growth and development, but whether and how PPSB affects nitrogen metabolism and starch accumulation has not been fully elucidated. In this study, we took the energy plant duckweed (strain Lemna turionifera 5511) as the research object and used a stable genetic transformation system to heterologously over-expressing Arabidopsis AtPSAT1 (the gene encoding phosphoserine aminotransferase, the second enzyme of PPSB). Our results showed that, under nitrogen starvation, the transgenic plants grew faster, with higher values of Fv/Fm, rETR, and Y(II), as well as fresh and dry weight, than the wild-type. More promisingly, the accumulation of starch was also found to be significantly improved when over-expressing AtPSAT1 in the transgenic plants. qRT-PCR analysis results showed that the expression of genes related to nitrogen assimilation, carbon metabolism, and starch biosynthesis was up-regulated, while the expression of starch degradation-related genes was down-regulated by AtPSAT1 over-expression. We propose that the increased starch accumulation caused by AtPSAT1 over-expression may result from both elevated photosynthetic capacity and nitrogen utilization efficiency. This research sheds new light on the mechanism underlying the ability of PPSB to coordinate nitrogen and carbon metabolism, and provides a feasible way to improve starch production, that is, through engineering PPSB in crops.
The Medium Composition Impacts Staphylococcus aureus Biofilm Formation and Susceptibility to Antibiotics Applied in the Treatment of Bone Infections
Authors: Paleczny Justyna , Brożyna Malwina , Dudek-Wicher Ruth , Dydak Karolina , Oleksy-Wawrzyniak Monika , Madziała Marcin , Bartoszewicz Marzenna , Junka Adam
Published: October 2022

The biofilm-associated infections of bones are life-threatening diseases, requiring application of dedicated antibiotics in order to counteract the tissue damage and spread of microorganisms. The in vitro analyses on biofilm formation and susceptibility to antibiotics are frequently carried out using methods that do not reflect conditions at the site of infection. To evaluate the influence of nutrient accessibility on Staphylococcus aureus biofilm development in vitro, a cohesive set of analyses in three different compositional media was performed. Next, the efficacy of four antibiotics used in bone infection treatment, including gentamycin, ciprofloxacin, levofloxacin, and vancomycin, against staphylococcal biofilm, was also assessed. The results show a significant reduction in the ability of biofilm to grow in a medium containing elements occurring in the serum, which also translated into the diversified changes in the efficacy of used antibiotics, compared to the setting in which conventional media were applied. The differences indicate the need for implementation of adequate in vitro models that closely mimic the infection site. The results of the present research may be considered an essential step toward the development of in vitro analyses aiming to accurately indicate the most suitable antibiotic to be applied against biofilm-related infections of bones.
Experimental and Computational Studies on the Interaction of a Dansyl-Based Fluorescent Schiff Base Ligand with Cu2+ Ions and CuO NPs
Authors: Sanmartín-Matalobos Jesús , Bermejo-Barrera Pilar , Pérez-Juste Ignacio , Fondo Matilde , García-Deibe Ana M. , Alves-Iglesias Yeneva
Published: October 2022

We studied the interaction of Cu2+ ions and CuO nanoparticles with the fluorescent Schiff base ligand H3L, which derives from the condensation of 4-formyl-3-hydroxybenzoic acid with N-(2-aminobenzyl)-5-(dimethylamino)naphthalene-1-sulfonamide (DsA). A detailed assignment of the most significant bands of the electronic and infrared spectra of H3L and DsA was performed using DFT methods, based on both crystal structures. The affinity of H3L to react with Cu2+ ions in solution (KB = 9.01 103 L mol−1) is similar to that found for the Cu2+ ions present on the surface of CuO NPs (KB = 9.84 103 L mol−1). Fluorescence spectroscopic measurements suggest five binding sites for H3L on the surface of the CuO NPs used. The µ-XRF analysis indicates that a polycrystalline sample of CuO-H3L NPs contains 15:1 Cu:S molar ratio (CuO:H3L). ATR-FTIR spectroscopy, supported by DFT calculations, showed that the HL2− (as a phenolate and sulfonamide anion) is coordinated to superficial Cu2+ ions of the CuO NPs through their azomethine, sulphonamide, and phenolic groups. A solution of H3L (126 ppb) shows sensitive responses to CuO NPs, with a limit of detection (LOD) of 330 ppb. The working range for detection of CuO NPs with [H3L] = 126 ppb was 1.1–9.5 ppm. Common metal ions in water, such as Na+, K+, Mg2+, Ca2+, Fe3+, and Al3+ species, do not interfere significantly with the detection of CuO NPs.
Effect of Neonicotinoid Pesticides on Japanese Water Systems: Review with Focus on Reproductive Toxicity
Authors: Terayama Hayato , Sakabe Kou , Kiyoshima Daisuke , Qu Ning , Sato Tsutomu , Suyama Kaori , Hayashi Shogo , Sakurai Kenichi , Todaka Emiko , Mori Chisato
Published: October 2022

Neonicotinoid pesticides (NPs) are neurotoxic substances. They are highly effective as insecticides owing to their water solubility, permeability, and long-lasting activity. These molecules are structurally similar to nicotine and act as nicotinic acetylcholine receptor agonists. The administration of NPs to experimental animals reportedly causes neuromuscular and reproductive disorders. Moreover, recently reported problems caused by NPs include damage to land-dwelling creatures (such as mammals and birds), hydrobiology, and ecosystems. This review summarizes the recent reports on NP concentrations detected in river systems in several Japanese regions. These values were lower than the environmental standard values; however, seasonal variations were observed. Furthermore, reports on NP-induced testicular and ovarian toxicity were examined, revealing that the mechanism of injury is mainly driven by oxidative stress. The use of NPs is declining worldwide, except in Japan; therefore, continuous monitoring remains necessary.
Biological Activity of fac-[Re(CO)3(phen)(aspirin)], fac-[Re(CO)3(phen)(indomethacin)] and Their Original Counterparts against Ishikawa and HEC-1A Endometrial Cancer Cells
Authors: Kuźmycz Olga , Kowalczyk Aleksandra , Stączek Paweł
Published: October 2022

Nonsteroidal anti-inflammatory drugs (NSAIDs) are inhibitors of cyclooxygenase enzyme (COX) and were found to have positive effects in reducing the risk of developing gynecological cancers. However, long-term administration of NSAIDs carries the risk of various side effects, including those in the digestive and circulatory systems. Therefore, there is a constant need to develop new NSAID derivatives. In this work, we investigated rhenium NSAIDs, comparing their effects on endometrial cancer cells with original NSAIDs, demonstrating the high activity of aspirin and indomethacin derivatives. The cytotoxic activity of rhenium derivatives against the Ishikawa and HEC-1A cancer cell lines was higher than that of the original NSAIDs. The IC50 after 24-h incubation of Ishikawa and HEC-1A were 188.06 µM and 394.06 µM for rhenium aspirin and 228.6 µM and 1459.3 µM for rhenium indomethacin, respectively. At the same time, IC50 of aspirin and indomethacin were 10,024.42 µM and 3295.3 µM for Ishikawa, and 27,255.8 µM and 5489.3 µM for HEC-1A, respectively. Moreover, these derivatives were found to inhibit the proliferation of both cell lines in a time- and state-dependent manner. The Ishikawa cell proliferation was strongly inhibited by rhenium aspirin and rhenium indomethacin after 72-h incubation (*** = p < 0.001), while the HEC-1A proliferation was inhibited by the same agents already after 24-h incubation (*** = p < 0.001). Furthermore, the ROS level in the mitochondria of the tested cells generated in the presence of rhenium derivatives was higher than the original NSAIDs. That was associated with rhenium indomethacin exclusively, which had a significant effect (*** = p < 0.001) on both Ishikawa and HEC-1A cancer cells. Rhenium aspirin had a significant effect (*** = p < 0.001) on the mitochondrial ROS level of Ishikawa cells only. Overall, the research revealed a high potential of the rhenium derivatives of aspirin and indomethacin against endometrial cancer cells compared with the original NSAIDs.
A Comprehensive Review on Beneficial Effects of Catechins on Secondary Mitochondrial Diseases
Authors: Chen Baoyi , Zhang Wenting , Lin Chuyuan , Zhang Lingyun
Published: October 2022

Mitochondria are the main sites for oxidative phosphorylation and synthesis of adenosine triphosphate in cells, and are known as cellular power factories. The phrase “secondary mitochondrial diseases” essentially refers to any abnormal mitochondrial function other than primary mitochondrial diseases, i.e., the process caused by the genes encoding the electron transport chain (ETC) proteins directly or impacting the production of the machinery needed for ETC. Mitochondrial diseases can cause adenosine triphosphate (ATP) synthesis disorder, an increase in oxygen free radicals, and intracellular redox imbalance. It can also induce apoptosis and, eventually, multi-system damage, which leads to neurodegenerative disease. The catechin compounds rich in tea have attracted much attention due to their effective antioxidant activity. Catechins, especially acetylated catechins such as epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), are able to protect mitochondria from reactive oxygen species. This review focuses on the role of catechins in regulating cell homeostasis, in which catechins act as a free radical scavenger and metal ion chelator, their protective mechanism on mitochondria, and the protective effect of catechins on mitochondrial deoxyribonucleic acid (DNA). This review highlights catechins and their effects on mitochondrial functional metabolic networks: regulating mitochondrial function and biogenesis, improving insulin resistance, regulating intracellular calcium homeostasis, and regulating epigenetic processes. Finally, the indirect beneficial effects of catechins on mitochondrial diseases are also illustrated by the warburg and the apoptosis effect. Some possible mechanisms are shown graphically. In addition, the bioavailability of catechins and peracetylated-catechins, free radical scavenging activity, mitochondrial activation ability of the high-molecular-weight polyphenol, and the mitochondrial activation factor were also discussed.