bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification, Characterization, and Expression Profiling of the Glutaredoxin Gene Family in Tea Plant (Camellia sinensis)
Authors: Jiang Dong , Yang Wenhai , Pi Jianhui , Yang Guoqun , Luo Yong , Du Shenxiu , Li Ning , Huang Li-Jun
Published: August 2023

Glutaredoxins (GRXs) are a widely distributed group of small oxidoreductases that play an important role in responding to oxidative stress and maintaining redox homeostasis in living organisms. However, there has been no report on the GRX gene family in tea plants (Camellia sinensis). In this study, we conducted a systematic analysis of the CsGRX gene family in tea plants and identified a total of 86 CsGRX genes. Based on phylogenetic and conserved active site analyses, these genes were classified into four categories: CC-type, CPYC-type, CGFS-type, and GRL-type. These subtypes showed distinct characteristics in terms of gene structure, conserved motif, chromosome distribution, subcellular localization, cis-regulatory elements, and expression pattern, indicating functional differences among CsGRX family members. Collinearity analysis showed that the CsGRX family may have undergone member expansion using tandem and segmental duplication along with overwhelmingly strict purifying selection. Protein tertiary structure analysis supported the conserved site-specific binding of CsGRX family members to glutathione. Protein interaction network analysis revealed that CsGRX may interact with glutathione reductase (GR), 2-Cys Peroxiredoxin BAS1, TGA3, and others to participate in the oxidative stress response in tea plants. GO and KEGG enrichment analyses also supported the important role of the CsGRX family in maintaining intracellular redox homeostasis. Expression analysis based on RNA-seq revealed differential expression patterns of CsGRX genes under drought, cold stress, and in different tissues, which were further confirmed by RT-qPCR analysis, indicating their broad-spectrum functionality. This study provides a new perspective for further exploring the evolution and molecular functions of specific CsGRX genes.
Quantum Machine Learning for Security Assessment in the Internet of Medical Things (IoMT)
Authors: Rajawat Anand Singh , Goyal S. B. , Bedi Pradeep , Jan Tony , Whaiduzzaman Md , Prasad Mukesh
Published: August 2023

Internet of Medical Things (IoMT) is an ecosystem composed of connected electronic items such as small sensors/actuators and other cyber-physical devices (CPDs) in medical services. When these devices are linked together, they can support patients through medical monitoring, analysis, and reporting in more autonomous and intelligent ways. The IoMT devices; however, often do not have sufficient computing resources onboard for service and security assurance while the medical services handle large quantities of sensitive and private health-related data. This leads to several research problems on how to improve security in IoMT systems. This paper focuses on quantum machine learning to assess security vulnerabilities in IoMT systems. This paper provides a comprehensive review of both traditional and quantum machine learning techniques in IoMT vulnerability assessment. This paper also proposes an innovative fused semi-supervised learning model, which is compared to the state-of-the-art traditional and quantum machine learning in an extensive experiment. The experiment shows the competitive performance of the proposed model against the state-of-the-art models and also highlights the usefulness of quantum machine learning in IoMT security assessments and its future applications.
Optimization of QuEChERS Extraction for Determination of Carotenoids, Polyphenols, and Sterols in Orange Juice Using Design of Experiments and Response Surface Methodology
Authors: Iwasaki Yusuke , Yamada Saki , Sakuma Shinya , Kanba Shunpei , Youda Chinatsu , Ono Mizuki , Ito Rie , Kamei Junzo , Akiyama Hiroshi
Published: August 2023

Several compounds with different physical properties are present in foods, biological components, and environmental samples, and there are cases in which these must be analyzed simultaneously. However, it is difficult to extract compounds with different physical properties from the same sample using a single method. In the present study, we examined the optimal conditions for the QuEChERS extraction of several kinds of compounds from orange juice using design of experiments (DoE) and response surface methodology (RSM) to determine the optimal ratio of organic solvent to sodium chloride. We determined the optimal extraction conditions, which were within the design space, using 100% tetrahydrofuran (THF) as the extraction organic solvent and NaCl:MgSO4 = 75:25 as the salt. The developed LC/MS/MS method using QuEChERS extraction achieved specific detection and precise quantification. Finally, we measured the polyphenols, sterols, and carotenoids in citrus juice using the optimized QuEChERS extraction method before LC/MS/MS analysis. Most of the analytes were quantifiable in orange juice. The optimized method achieved ease of operation, the extraction of analytes from food samples in a short time (within 30 min), minimization of analytical residues, and reliability. The DoE and RSM approach may contribute to better optimization of the extraction conditions for the lowest number of experiments.
Duopoly and Endogenous Single Product Quality Strategies
Authors: Gayer Amit
Published: August 2023

This research paper investigates a duopolistic market characterized by vertical product differentiation. The study considers both product qualities and consumer preferences represented as one-dimensional intervals. The focus is on analyzing the equilibrium in a duopoly game with convex production costs. In this setting, each firm has the option to present a multi-product strategy consisting of quality–price pairs, and their profits are determined by the decisions made by consumers. The findings of the study reveal that, under typical consumer preference conditions, both firms tend to offer a single quality–price pair. Additionally, the market is shown to be fully served, and firm profits decrease as the index of product quality increases. A comparative analysis is also conducted with the case of a monopoly.
Cognitive–Behavioral Profile in Pediatric Patients with Syndrome 5p-; Genotype–Phenotype Correlationships
Authors: Bel-Fenellós Cristina , Biencinto-López Chantal , Sáenz-Rico Belén , Hernández Adolfo , Sandoval-Talamantes Ana Karen , Tenorio-Castaño Jair , Lapunzina Pablo , Nevado Julián
Published: August 2023

(1) Background: 5p minus Syndrome (S5p-) is a neurodevelopmental disorder caused by a deletion in the short arm of chromosome 5. Among the phenotypic characteristics of S5p-, the most characteristic and representative element is a monochromatic cry with a high-pitched tone reminiscent of a cat’s meow. Individuals may also show great phenotypic heterogeneity and great genetic variability. Regarding cognitive–behavioral aspects of the syndrome, the studies are scarce and do not establish a general profile of the main cognitive–behavioral particularities that this syndrome presents. The main objective of this work was to describe the development profile of a cohort of 45 children with 5p minus Syndrome, concerning the biomedical, genetic, cognitive, and behavioral aspects. Establishing putative genotype–phenotype (cognitive–behavioral profiles) relationships in our cohort, from an interdisciplinary approach. (2) Methods: A selection of instruments of measures was selected for neuropsychological assessment (3) Results: In general, children with S5p- have a higher cognitive level than a communicative and motor level. Language difficulties, especially expressive ones, influence the frequency and severity of the most frequent behavioral problems in S5p. The most significant problem behavior of children with S5p-, especially girls, is self-harm. Compulsive behavior, limited preferences, and interest in monotony are significantly more frequent in subjects with better cognitive levels. We also find a significant correlation between the size of the loss of genetic material on 5p and the cognitive level of the subjects. (4) Conclusions: We described for the first time, the cognitive–behavioral profile of a cohort of minors with S5p-. Remarkably, it was found that language, especially of an expressive nature, modulates the most frequent behavioral aspects in subjects with lower cognitive levels, so it is essential to develop verbal or alternative communication strategies adjusted to these individuals.
Normal-Weight Obesity and Metabolic Syndrome in Korean Adults: A Population-Based Cross-Sectional Study
Authors: Kim Jeonghyeon , Kang Seamon , Kang Hyunsik
Published: August 2023

Background: The disadvantage of using body mass index (BMI) as an obesity diagnostic tool is that it cannot distinguish between fat mass and lean mass, which may understate the impact of obesity on metabolic complications. This population-based cross-sectional study aimed to investigate the relationship between normal-weight obesity (NWO) and metabolic syndrome in Korean adults aged 20 years (5962 males and 6558 females) who took part in the Korea National Health and Nutrition Examination Surveys from 2008 to 2011. Methods: NWO was defined as having a BMI of 18.5 to 24.9 kg/m2 and a body fat percentage of 26.0% in males or 36.0% in females. Metabolic syndrome (MetS) was defined using the revised National Cholesterol Education Program definition, with a Korean-specific waist circumference threshold of >90 cm for males and >85 cm for females. Results: NWO males and females were 2.7 times (p < 0.001) and 1.9 times (p < 0.001) more likely to develop metabolic syndrome than normal-weight non-obese males and females, respectively. Additionally, NWO females were 1.3 times (p = 0.027) more likely to develop MetS even after adjustments for all measured covariates. Conclusions: The current findings of the study show that NWO is a proxy biomarker of MetS to be considered for early intervention.
Institutional Resistance to Medical Assistance in Dying in Canada: Arguments and Realities Emerging in the Public Domain
Authors: Knox Michelle , Wagg Adrian
Published: August 2023

Since the legalization of medical assistance in dying (MAiD) in Canada in 2016, volitional non-participation in MAiD on the part of some healthcare institutions has revealed ethical uncertainties, potential access problems, and policy gaps. The problem has remained much neglected in the literature base, with no comprehensive studies on the subject so far. We analyzed print media articles and grey literature on institutional objections to and non-participation in MAiD. Thematic analyses were performed on all data to better understand the diverse stakeholder arguments and positions that characterize this important public health debate. Our search yielded 89 relevant media articles and 22 legislative, policy, and other relevant documents published since 2016 in the English language. We identified four main themes about institutional refusals to participate in MAiD, articulated as the following questions: (1) Who has the right to conscience? (2) Can MAiD be considered a palliative practice? (3) Are there imbalances across diverse stakeholder rights and burdens? and (4) Where are the gaps being felt in MAiD service implementation? Stakeholder views about institutional conscience with respect to MAiD are varied, complex, and evolving. In the absence of substantial systematic evidence, public domain materials constitute a key resource for understanding the implications for service access and determining the relevance of this contentious issue for future MAiD research and policy.
Test–Retest Reliability, Agreement and Criterion Validity of Three Questionnaires for the Assessment of Physical Activity and Sedentary Time in Patients with Myocardial Infarction
Authors: Bargholtz Marcus , Brosved Madeleine , Heimburg Katarina , Hellmark Marie , Leosdottir Margret , Hagströmer Maria , Bäck Maria
Published: August 2023

Regular physical activity (PA) and limited sedentary time (SED) are highly recommended in international guidelines for patients after a myocardial infarction (MI). Data on PA and SED are often self-reported in clinical practice and, hence, reliable and valid questionnaires are crucial. This study aimed to assess the test–retest reliability, criterion validity and agreement of two PA and one SED questionnaire commonly used in clinical practice, developed by the Swedish National Board of Health and Welfare (BHW) and the Swedish national quality register SWEDEHEART. Data from 57 patients (mean age 66 ± 9.2 years, 42 males) was included in this multi-centre study. The patients answered three questionnaires on PA and SED at seven-day intervals and wore an accelerometer for seven days. Test–retest reliability, criterion validity and agreement were assessed using Spearman’s rho and linearly weighted kappa. Test–retest reliability was moderate for three of the six-sub questions (k = 0.43–0.54) within the PA questionnaires. For criterion validity, the correlation was fair within three of the six sub-questions (r = 0.41–0.50) within the PA questionnaires. The SED questionnaire had low agreement (k = 0.12) and criterion validity (r = 0.30). The studied questionnaires for PA could be used in clinical practice as a screening tool and/or to evaluate the level of PA in patients with an MI. Future research is recommended to develop and/or evaluate SED questionnaires in patients with an MI.
Anatomic Features of the Nasal and Pharyngeal Region Do Not Influence PAP Therapy Response
Authors: Seifen Christopher , Schlaier Nadine Angelina , Pordzik Johannes , Staufenberg Anna-Rebekka , Matthias Christoph , Gouveris Haralampos , Bahr-Hamm Katharina
Published: August 2023

The objective of this study was to investigate to which extent anatomic features of the nasal and pharyngeal region contribute to the severity of obstructive sleep apnea (OSA) and positive airway pressure (PAP) therapy response. Therefore, 93 patients (mean age 57.5 ± 13.0 years, mean body mass index 32.2 ± 5.80 kg/m2, 75 males, 18 females) diagnosed with OSA who subsequently started PAP therapy were randomly selected from the databank of a sleep laboratory of a tertiary university medical center. Patients were subdivided based on nasal anatomy (septal deviation, turbinate hyperplasia, their combination, or none of the above), pharyngeal anatomy (webbing, tonsillar hyperplasia, their combination, or none of the above), and (as a separate group) tongue base anatomy (no tongue base hyperplasia or tongue base hyperplasia). Then, polysomnographic data (e.g., arousal index, ARI; respiratory disturbance index, RDI; apnea index, AI; hypopnea index, HI; and oxygen desaturation index, ODI) of diagnostic polysomnography (PSG) and PAP therapy control PSG were collected, grouped, and evaluated. Septal deviation, turbinate hyperplasia, or their combination did not significantly affect the assessed PSG parameters or the response to PAP therapy compared with patients without nasal obstruction (p > 0.05 for all parameters). Accordingly, most PSG parameters and the response to PAP therapy were not significantly affected by webbing, tonsil hyperplasia, or their combination compared with patients without pharyngeal obstruction (p > 0.05 for RDI, AI, HI, and ODI, respectively). However, in the pharyngeal anatomy group, ARI was significantly higher in patients with tonsil hyperplasia (p = 0.018). Further, patients with tongue base hyperplasia showed a significantly higher HI in the diagnostic PSG (p = 0.025) compared with patients with normal tongue base anatomy, but tongue base anatomy did not significantly affect the response to PAP therapy (p > 0.05 for all parameters). The influence of anatomic features of the nasal and pharyngeal region on PAP therapy response appears to be small, and generalizability of these results requires further studies.
Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses
Authors: Park Jihyun , Nguyen Thi Minh Nguyet , Park Hyun-ah , Nguyen My Tuyen Thi , Lee Nan-young , Ban So-young , Park Kyu-been , Lee Chang-kyu , Kim Jaehan , Park Jong-Tae
Published: August 2023

Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.
Genetic and Epigenetic Features of Uveal Melanoma—An Overview and Clinical Implications
Authors: Pašalić Daria , Nikuševa-Martić Tamara , Sekovanić Ankica , Kaštelan Snježana
Published: August 2023

Uveal melanoma (UM) is rare, but it is the most common primary intraocular malignancy among adults. This review represents the molecular, genetic, and immunobiological mechanisms involved in UM carcinogenesis and progression, as well as data about the association of chromosomal changes, genetic mutations, selective proteins, and biochemical biomarkers with the clinical implications of UM. Genetic analysis has the potential to identify patients with a high risk of UM metastasis, enabling management that is more effective and allowing for the follow-up of patients. Advancements in molecular characterization of UM offer opportunities to develop targeted therapeutic strategies by focusing on relevant signaling pathways. Changes in miRNA expression could be useful in the diagnosis and prognosis of UM, due to unique miRNA profiles in melanoma cells or tissue and its association with metastasis. Although liver function tests do not provide enough data on the prognosis of UM, due to the high frequency of liver metastasis, liver function tests (LFTs) might be useful indicators; however, the absence of rising LFT values cannot lead to the exclusion of liver metastases. Molecular analysis of tumor tissue will allow us to identify patients with the added benefit of new therapeutic agents and provide a better insight into melanoma pathogenesis and its biological behavior.
Adsorption of Ferritin at Nanofaceted Al2O3 Surfaces
Authors: Pothineni Bhanu K. , Kollmann Sabrina , Li Xinyang , Grundmeier Guido , Erb Denise J. , Keller Adrian
Published: August 2023

The influence of nanoscale surface topography on protein adsorption is highly important for numerous applications in medicine and technology. Herein, ferritin adsorption at flat and nanofaceted, single-crystalline Al2O3 surfaces is investigated using atomic force microscopy and X-ray photoelectron spectroscopy. The nanofaceted surfaces are generated by the thermal annealing of Al2O3 wafers at temperatures above 1000 °C, which leads to the formation of faceted saw-tooth-like surface topographies with periodicities of about 160 nm and amplitudes of about 15 nm. Ferritin adsorption at these nanofaceted surfaces is notably suppressed compared to the flat surface at a concentration of 10 mg/mL, which is attributed to lower adsorption affinities of the newly formed facets. Consequently, adsorption is restricted mostly to the pattern grooves, where the proteins can maximize their contact area with the surface. However, this effect depends on the protein concentration, with an inverse trend being observed at 30 mg/mL. Furthermore, different ferritin adsorption behavior is observed at topographically similar nanofacet patterns fabricated at different annealing temperatures and attributed to different step and kink densities. These results demonstrate that while protein adsorption at solid surfaces can be notably affected by nanofacet patterns, fine-tuning protein adsorption in this way requires the precise control of facet properties.
Anti-Melanogenic and Antioxidant Activity of Bifidobacterium longum Strain ZJ1 Extracts, Isolated from a Chinese Centenarian
Authors: Wu Jing , Zhang Funa , Yu Haixia , Qi Shimei , Wu Yu , Xiao Weihua
Published: August 2023

Melanin produced by melanocytes protects our skin against ultraviolet (UV) radiation-induced cell damage and oxidative stress. Melanin overproduction by hyperactivated melanocytes is the direct cause of skin hyperpigmentary disorders, such as freckles and melasma. Exploring natural whitening agents without the concern of toxicity has been highly desired. In this study, we focused on a Bifidobacterium longum strain, ZJ1, isolated from a Chinese centenarian, and we evaluated the anti-melanogenic activity of the distinctive extracts of ZJ1. Our results demonstrated that whole lysate (WL) and bacterial lysate (BL) of ZJ1 ferments efficiently reduce α-melanocyte-stimulating hormone (α-MSH)-induced melanin production in B16-F10 cells as well as the melanin content in zebrafish embryos. BL and WL downregulate melanogenesis-related gene expression and indirectly inhibit intracellular tyrosinase activity. Furthermore, they both showed antioxidant activity in a menadione-induced zebrafish embryo model. Our results suggest that ZJ1 fermentation lysates have application potential as therapeutic reagents for hyperpigmentary disorders and whitening agents for cosmetics.
Furoquinoline Alkaloids: Insights into Chemistry, Occurrence, and Biological Properties
Authors: Szewczyk Agnieszka , Pęczek Filip
Published: August 2023

Furoquinoline alkaloids exhibit a diverse range of effects, making them potential candidates for medicinal applications. Several compounds within this group have demonstrated antimicrobial and antiprotozoal properties. Of great interest is their potential as acetylcholinesterase inhibitors and anti-inflammatory agents in neurodegenerative diseases. The promising biological properties of furoquinoline alkaloids have motivated extensive research in this field. As a result, new compounds have been isolated from this group of secondary metabolites, and numerous pharmacological studies have been conducted to investigate their activity. It is crucial to understand the mechanisms of action of furoquinoline alkaloids due to their potential toxicity. Further research is required to elucidate their mechanisms of action and metabolism. Additionally, the exploration of derivative compounds holds significant potential in enhancing their pharmacological benefits. In vitro plant cultures offer an alternative approach to obtaining alkaloids from plant material, presenting a promising avenue for future investigations.
Inhibition of USP2 Enhances TRAIL-Mediated Cancer Cell Death through Downregulation of Survivin
Authors: Lee Tak Gyeom , Woo Seon Min , Seo Seung Un , Kim Shin , Park Jong-Wook , Chang Young-Chae , Kwon Taeg Kyu
Published: August 2023

Ubiquitin-specific protease 2 (USP2) is a deubiquitinase belonging to the USPs subfamily. USP2 has been known to display various biological effects including tumorigenesis and inflammation. Therefore, we aimed to examine the sensitization effect of USP2 in TRAIL-mediated apoptosis. The pharmacological inhibitor (ML364) and siRNA targeting USP2 enhanced TNF-related apoptosis-inducing ligand (TRAIL)-induced cancer cell death, but not normal cells. Mechanistically, USP2 interacted with survivin, and ML364 degraded survivin protein expression by increasing the ubiquitination of survivin. Overexpression of survivin or USP2 significantly prevented apoptosis through cotreatment with ML364 and TRAIL, whereas a knockdown of USP2 increased sensitivity to TRAIL. Taken together, our data suggested that ML364 ubiquitylates and degrades survivin, thereby increasing the reactivity to TRAIL-mediated apoptosis in cancer cells.
Recent Progress of Spectroscopic Probes for Peroxynitrite and Their Potential Medical Diagnostic Applications
Authors: Liu Zixin , Mo Shanyan , Hao Zhenming , Hu Liming
Published: August 2023

Peroxynitrite (ONOO−) is a crucial reactive oxygen species that plays a vital role in cellular signal transduction and homeostatic regulation. Determining and visualizing peroxynitrite accurately in biological systems is important for understanding its roles in physiological and pathological activity. Among the various detection methods, fluorescent probe-based spectroscopic detection offers real-time and minimally invasive detection, high sensitivity and selectivity, and easy structural and property modification. This review categorizes fluorescent probes by their fluorophore structures, highlighting their chemical structures, recognition mechanisms, and response behaviors in detail. We hope that this review could help trigger novel ideas for potential medical diagnostic applications of peroxynitrite-related molecular diseases.
Multigenerational Effects of Graphene Oxide Nanoparticles on Acheta domesticus DNA Stability
Authors: Flasz Barbara , Ajay Amrendra K. , Tarnawska Monika , Babczyńska Agnieszka , Majchrzycki Łukasz , Kędziorski Andrzej , Napora-Rutkowski Łukasz , Świerczek Ewa , Augustyniak Maria
Published: August 2023

The use of nanoparticles like graphene oxide (GO) in nanocomposite industries is growing very fast. There is a strong concern that GO can enter the environment and become nanopollutatnt. Environmental pollutants’ exposure usually relates to low concentrations but may last for a long time and impact following generations. Attention should be paid to the effects of nanoparticles, especially on the DNA stability passed on to the offspring. We investigated the multigenerational effects on two strains (wild and long-lived) of house cricket intoxicated with low GO concentrations over five generations, followed by one recovery generation. Our investigation focused on oxidative stress parameters, specifically AP sites (apurinic/apyrimidinic sites) and 8-OHdG (8-hydroxy-2′-deoxyguanosine), and examined the global DNA methylation pattern. Five intoxicated generations were able to overcome the oxidative stress, showing that relatively low doses of GO have a moderate effect on the house cricket (8-OHdG and AP sites). The last recovery generation that experienced a transition from contaminated to uncontaminated food presented greater DNA damage. The pattern of DNA methylation was comparable in every generation, suggesting that other epigenetic mechanisms might be involved.
AMPK/FOXO3a Pathway Increases Activity and/or Expression of ATM, DNA-PKcs, Src, EGFR, PDK1, and SOD2 and Induces Radioresistance under Nutrient Starvation
Authors: Urushihara Yusuke , Hashimoto Takuma , Fujishima Yohei , Hosoi Yoshio
Published: August 2023

Most solid tumors contain hypoxic and nutrient-deprived microenvironments. The cancer cells in these microenvironments have been reported to exhibit radioresistance. We have previously reported that nutrient starvation increases the expression and/or activity of ATM and DNA-PKcs, which are involved in the repair of DNA double-strand breaks induced by ionizing radiation. In the present study, to elucidate the molecular mechanisms underlying these phenomena, we investigated the roles of AMPK and FOXO3a, which play key roles in the cellular response to nutrient starvation. Nutrient starvation increased clonogenic cell survival after irradiation and increased the activity and/or expression of AMPKα, FOXO3a, ATM, DNA-PKcs, Src, EGFR, PDK1, and SOD2 in MDA-MB-231 cells. Knockdown of AMPKα using siRNA suppressed the activity and/or expression of FOXO3a, ATM, DNA-PKcs, Src, EGFR, PDK1, and SOD2 under nutrient starvation. Knockdown of FOXO3a using siRNA suppressed the activity and/or expression of AMPKα, ATM, DNA-PKcs, FOXO3a, Src, EGFR, PDK1, and SOD2 under nutrient starvation. Nutrient starvation decreased the incidence of apoptosis after 8 Gy irradiation. Knockdown of FOXO3a increased the incidence of apoptosis after irradiation under nutrient starvation. AMPK and FOXO3a appear to be key molecules that induce radioresistance under nutrient starvation and may serve as targets for radiosensitization.
Glucagon and Its Receptors in the Mammalian Heart
Authors: Neumann Joachim , Hofmann Britt , Dhein Stefan , Gergs Ulrich
Published: August 2023

Glucagon exerts effects on the mammalian heart. These effects include alterations in the force of contraction, beating rate, and changes in the cardiac conduction system axis. The cardiac effects of glucagon vary according to species, region, age, and concomitant disease. Depending on the species and region studied, the contractile effects of glucagon can be robust, modest, or even absent. Glucagon is detected in the mammalian heart and might act with an autocrine or paracrine effect on the cardiac glucagon receptors. The glucagon levels in the blood and glucagon receptor levels in the heart can change with disease or simultaneous drug application. Glucagon might signal via the glucagon receptors but, albeit less potently, glucagon might also signal via glucagon-like-peptide-1-receptors (GLP1-receptors). Glucagon receptors signal in a species- and region-dependent fashion. Small molecules or antibodies act as antagonists to glucagon receptors, which may become an additional treatment option for diabetes mellitus. Hence, a novel review of the role of glucagon and the glucagon receptors in the mammalian heart, with an eye on the mouse and human heart, appears relevant. Mouse hearts are addressed here because they can be easily genetically modified to generate mice that may serve as models for better studying the human glucagon receptor.
InterviewBot: Real-Time End-to-End Dialogue System for Interviewing Students for College Admission
Authors: Wang Zihao , Keyes Nathan , Crawford Terry , Choi Jinho D.
Published: August 2023

We present the InterviewBot, which dynamically integrates conversation history and customized topics into a coherent embedding space to conduct 10 min hybrid-domain (open and closed) conversations with foreign students applying to U.S. colleges to assess their academic and cultural readiness. To build a neural-based end-to-end dialogue model, 7361 audio recordings of human-to-human interviews are automatically transcribed, where 440 are manually corrected for finetuning and evaluation. To overcome the input/output size limit of a transformer-based encoder–decoder model, two new methods are proposed, context attention and topic storing, allowing the model to make relevant and consistent interactions. Our final model is tested both statistically by comparing its responses to the interview data and dynamically by inviting professional interviewers and various students to interact with it in real-time, finding it highly satisfactory in fluency and context awareness.
MicroRNA miR-274-5p Suppresses Found-in-Neurons Associated with Melanotic Mass Formation and Developmental Growth in Drosophila
Authors: Kim Hee Kyung , Kim Chae Jeong , Jang Daegyu , Lim Do-Hwan
Published: August 2023

The hematopoietic system plays a crucial role in immune defense response and normal development, and it is regulated by various factors from other tissues. The dysregulation of hematopoiesis is associated with melanotic mass formation; however, the molecular mechanisms underlying this process are poorly understood. Here, we observed that the overexpression of miR-274 in the fat body resulted in the formation of melanotic masses. Moreover, abnormal activation of the JNK and JAK/STAT signaling pathways was linked to these consequences. In addition to this defect, miR-274 overexpression in the larval fat body decreased the total tissue size, leading to a reduction in body weight. miR-274-5p was found to directly suppress the expression of found-in-neurons (fne), which encodes an RNA-binding protein. Similar to the effects of miR-274 overexpression, fne depletion led to melanotic mass formation and growth reduction. Collectively, miR-274 plays a regulatory role in the fne–JNK signaling axis in melanotic mass formation and growth control.
Lung Transplantation for Pulmonary Arterial Hypertension: Optimized Referral and Listing Based on an Evolving Disease Concept
Authors: Hu Xiaokun , Ding Ningying , Songchen Wanqiu , Wang Ruifeng , Chen Jing , Zhong Ailing , Nan Jinzhu , Zuo Yujie , Huang Heng , Tian Dong
Published: August 2023

Pulmonary hypertension (PH) was once a devastating and fatal disease entity, the outlook of which has been significantly improved by the continued progress of medical treatment algorithms. However, some patients still ultimately fail to achieve an adequate clinical response despite receiving maximal medical treatment. Historically, lung transplantation (LTx) has been the only effective therapeutic option that could lead to satisfactory outcomes and save these advanced patients’ lives. However, patients with PH tend to have the highest mortality rates on the transplant waiting list; especially after comprehensive medical treatment, they continue to deteriorate very rapidly, eventually missing optimal transplantation windows. Balancing optimized medical treatment with the appropriate timing of referral and listing has been highly controversial in LTx for patients with PH. The 2021 consensus document for the selection of lung transplant candidates from the International Society for Heart and Lung Transplantation (ISHLT) updated the specific recommendations for the LTx referral and listing time for patients with PH based on objective risk stratification. Herein, we review the evolving PH-related concepts and highlight the optimization of LTx referral and listing for patients with PH, as well as their management on the waiting list.
Occupational Medicine and Prevention of Chronic and Infectious Diseases
Authors: La Torre Giuseppe , Shaholli David , Colaprico Corrado , Manai Maria Vittoria , Ammirati Salvatore , Mantione Giorgia , Sernia Sabina
Published: August 2023

Occupational medicine is a clinical discipline that draws attention to the health of workers and their ability to work [...]
Biomechanical Implications of Mandibular Flexion on Implant-Supported Full-Arch Rehabilitations: A Systematic Literature Review
Authors: Caggiano Mario , D’Ambrosio Francesco , Acerra Alfonso , Giudice David , Giordano Francesco
Published: August 2023

Background: Mandibular flexion (MF) is a complex biomechanical phenomenon, which involves a deformation of the mandible, due mainly to the contraction of the masticatory muscles, and it can have numerous clinical effects. The deformation of the lower jaw caused by mandibular flexion is generally very small, and it is often overlooked and considered irrelevant from a clinical point of view by many authors; however, it should be important to remember that median mandibular flexure (MMF) has a multifactorial aetiology. The main aim of the current systematic review is to highlight the different factors that can increase MF in order to help clinicians identify patients to whom they should pay more attention. As a secondary outcome, we wanted to analyse the preventive measures and suitable techniques to be adopted to minimise the negative effects of this phenomenon on oral fixed rehabilitations. Methods: The review, which was carried out in accordance with the “Preferred Reporting Items for Systematic reviews and Meta-Analyses” (PRISMA) flowchart, was recorded in the “International Prospective Register of Systematic Reviews” (PROSPERO). As research questions, “Patient/Population, Intervention, Comparison and Outcomes” (PICO) questions were employed. Using the ROBINS-I technique, the risk of bias in non-randomised clinical studies was evaluated. Results: The initial electronic search identified over 1300 potential articles, of which 54 studies were included in this systematic review. Information regarding the relationship between MF and individual factors, mandibular movements, impression taking, and fixed rehabilitations were obtained. Conclusions: The studies included in this systematic review showed that MF is greater during protrusive movements, in the posterior areas of the lower jaw, and in patients with brachial facial type, greater jaw length; small gonial angle; and less density, length, and bone surface of the symphysis. The biomechanical effects of mandibular flexion on fixed restorations are debated. Prospective clinical and radiological observational studies should be conducted to evaluate the potential short-, medium-, and long-term consequences of MF.
Special Issue: “Cardiovascular Complications in Renal Diseases”
Authors: Burlacu Alexandru , Covic Adrian
Published: August 2023

The intricate interplay between cardiovascular (CV) pathology and chronic kidney disease (CKD) encompasses diagnostic protocols (both clinical and paraclinical), outcome assessments (such as mortality, morbidity, and costs), as well as advancements in new therapeutic approaches (including pharmacological, interventional, and surgical modalities) [...]
Rehabilitation for Musculoskeletal Disorders: The Emergence of Serious Games and the Promise of Personalized Versions Using Artificial Intelligence
Authors: Favre Julien , Cantaloube Alexis , Jolles Brigitte M.
Published: August 2023

According to the World Health Organization (WHO), musculoskeletal conditions are among the most common health problems, affecting approximately 1 [...]
Personalized Psychological Interventions in Children: Harnessing Advancements in Genetic and Epigenetic Research
Authors: Cerniglia Luca
Published: August 2023

Advancements in genetic and epigenetic research have opened new avenues for personalized psychological intervention in children [...]
Clinical Outcomes and Prognostic Factors in Complex, High-Risk Indicated Procedure (CHIP) and High-Bleeding-Risk (HBR) Patients Undergoing Percutaneous Coronary Intervention with Sirolimus-Eluting Stent Implantation: 4-Year Results
Authors: Tyczyński Maciej , Kern Adam , Buller Patryk , Wańha Wojciech , Gil Robert J. , Bil Jacek
Published: August 2023

We aimed to characterize the performance and safety of percutaneous coronary intervention (PCI) in complex, high-risk indicated procedure (CHIP) and high-bleeding-risk (HBR) patients at a 4-year follow up. We included all consecutive patients who underwent PCI with the sirolimus-eluting coronary stent Alex Plus (Balton, Poland) between July 2015 and March 2016. We analyzed various baseline demographic and clinical characteristics, laboratory data, and clinical outcomes. We enrolled 232 patients in whom 282 stents were implanted, including 81 patients meeting the CHIP criteria and 76 patients meeting the HBR criteria. In the whole population, the mean age was 68 ± 11 years, and 23.7% were females. Most procedures were performed from radial access (83.2%) using a 6F guiding catheter (95.7%). The lesions were mostly predilated (61.6%), and postdilatation was performed in 37.9%. The device success was 99.6% (in one case, a second stent was required due to heavy calcifications). Additional stents were deployed in 39% of cases due to edge dissection (6.9%), side branch stenting (5.2%), or diffuse disease (26.9%). Myocardial infarction (MI) type 4a was revealed in 2.2% of cases. At 4 years, the MACE rates for the whole population and for CHIP and HBR patients were 23.3%, 29.6%, and 27.6%, respectively. CHIP patients had a higher risk of MACEs (29.6% vs. 19.9%, HR 1.69, p = 0.032) and cardiac death (11.1% vs. 4.6%, HR 2.50, p = 0.048). There were no differences for MI (7.4% vs. 6.6%, p = 0.826) and TLR (18.5% vs. 12.6%, p = 0.150). HBR patients were also characterized by a higher risk of MACEs (27.6% vs. 21.2%, HR 1.84, p = 0.049) and cardiac death (17.1% vs. 1.9%, HR 9.61, p < 0.001). There were no differences for MI (7.9% vs. 6.4%, p = 0.669) and TLR (11.8% vs. 16.0%, p = 0.991). PCI in CHIP and HBR patients is feasible with a low rate of periprocedural complications. Nevertheless, CHIP and HBR patients are at a high risk of future adverse events and require strict surveillance to improve outcomes.
Research on an Underwater Target-Tracking Method Based on Zernike Moment Feature Matching
Authors: Gao Wenhan , Zhou Shanmin , Liu Shuo , Wang Tao , Zhang Bingbing , Xia Tian , Cai Yong , Leng Jianxing
Published: August 2023

Sonar images have the characteristics of lower resolution and blurrier edges compared to optical images, which make the feature-matching method in underwater target tracking less robust. To solve this problem, we propose a particle filter (PF)-based underwater target-tracking method utilizing Zernike moment feature matching. Zernike moments are used to construct the feature-description vector for feature matching and contribute to the update of particle weights. In addition, the particle state transition method is optimized by using a first-order autoregressive model. In this paper, we compare Hu moments and Zernike moments, and we also compare whether to optimize the particle state transition on the tracking results or not based on the effects of each option. The experimental results based on the AUV (autonomous underwater vehicle) prove that the robustness and accuracy of this innovative method is better than the other combined methods mentioned in this paper.
The Microbiome and Antibiotic Resistome in Soil under Biodegradable Composite Carbon Source Amendment
Authors: Yang Zhongchen , Lou Yanhong , Yan Xianghui , Pan Hong , Wang Hui , Yang Quangang , Sun Yajie , Zhuge Yuping
Published: August 2023

The decomposition of biodegradable composite carbon sources generates a large amount of biodegradable microplastics, which may not only furnish microbial denitrification, but might also pose potential environmental risks. In the present study, the effects of different dosages of a biodegradable composite carbon source on the microbial communities, the nitrogen metabolic pathways and the antibiotic resistome were explored through Illumina MiSeq sequencing analysis and metagenomic analysis. The results of partial least-square discriminant analysis (PLS-DA) and analysis of similarity (ANOSIM) demonstrated that the response of the bacterial community to a biodegradable composite carbon source was more obvious than the fungal community. The application of biodegradable microplastics diminished the complexity of the microbial communities to some extent and obviously stimulated denitrification. Antibiotics resistance gene (ARG) dispersal was not evidently accelerated after the addition of biodegradable composite carbon source. Lysobacter, Methylobacillus, Phyllobacterium, Sinorhizobium, Sphingomonas from Proteobacteria and Actinomadura, Agromyces, Gaiella and Micromonospora from Actinobacteria were the major ARG hosts. Overall, the addition of a biodegradable composite carbon source shaped microbial communities and their antibiotic resistance profiles in this study.
Charting the Path: Navigating Embryonic Development to Potentially Safeguard against Congenital Heart Defects
Authors: Bragança José , Pinto Rute , Silva Bárbara , Marques Nuno , Leitão Helena S. , Fernandes Mónica T.
Published: August 2023

Congenital heart diseases (CHDs) are structural or functional defects present at birth due to improper heart development. Current therapeutic approaches to treating severe CHDs are primarily palliative surgical interventions during the peri- or prenatal stages, when the heart has fully developed from faulty embryogenesis. However, earlier interventions during embryonic development have the potential for better outcomes, as demonstrated by fetal cardiac interventions performed in utero, which have shown improved neonatal and prenatal survival rates, as well as reduced lifelong morbidity. Extensive research on heart development has identified key steps, cellular players, and the intricate network of signaling pathways and transcription factors governing cardiogenesis. Additionally, some reports have indicated that certain adverse genetic and environmental conditions leading to heart malformations and embryonic death may be amendable through the activation of alternative mechanisms. This review first highlights key molecular and cellular processes involved in heart development. Subsequently, it explores the potential for future therapeutic strategies, targeting early embryonic stages, to prevent CHDs, through the delivery of biomolecules or exosomes to compensate for faulty cardiogenic mechanisms. Implementing such non-surgical interventions during early gestation may offer a prophylactic approach toward reducing the occurrence and severity of CHDs.
Energy and the Macrodynamics of Agrarian Societies
Authors: Karakatsanis Georgios , Mamassis Nikos
Published: August 2023

For the present work, we utilized Leslie White’s anthropological theory of cultural evolutionism as a theoretical benchmark for econometrically assessing the macrodynamics of energy use in agrarian societies that constituted the human civilization’s second energy paradigm between 12,000 BC and 1800 AC. As White’s theory views a society’s ability to harness and control energy from its environment as the primary function of culture, we may classify the evolution of human civilizations in three phases according to their energy paradigm, defined as the dominant pattern of energy harvesting from nature. In this context, we may distinguish three energy paradigms so far: hunting–gathering, agriculture, and fossil fuels. Agriculture, as humanity’s energy paradigm for ~14,000 years, essentially comprises a secondary form of solar energy that is biochemically transformed by photosynthetic life (plants and land). Based on this property, we model agrarian societies with similar principles to natural ecosystems. Just like natural ecosystems, agrarian societies receive abundant solar energy input but also have limited land ability to transform and store them biochemically. As in natural ecosystems, this constraint is depicted by the carrying capacity emerging biophysically from the limiting factor. Hence, the historical dynamics of agrarian societies are essentially reduced to their struggle to maximize energy use by maximizing the area and productivity of fertile land –in the role of a solar energy transformation hub– mitigating their limiting factor. Such an evolutionary forcing introduced technical upgrades, like the leverage of domesticated livestock power as a multiplier of the caloric value harvested by arable and grazing land combined. According to the above, we tested the econometric performance of four selected dynamic maps used extensively in ecology to reproduce humanity’s energy harvesting macrodynamics between 10,000 BC and 1800 AC: (a) the logistic map, (b) the logistic growth map, (c) a lower limiting case of the Hassel map that yields the Ricker map, and (d) a higher limiting case of the Hassel map that yields the Beverton–Holt map. Following our results, we discuss thoroughly our framework’s major elaborations on social hierarchy and competition as mechanisms for allocating available energy in society, as well as the related future research and econometric modeling challenges.