bluuebunny/crossref_metadata_2025 huggingface dataset:
The Spatial Effects of Regional Poverty: Spatial Dependence, Spatial Heterogeneity and Scale Effects
Authors: Liu Mengxiao , Ge Yong , Hu Shan , Hao Haiguang
Published: December 2023

Recognizing the spatial effects of regional poverty is essential for achieving sustainable poverty alleviation. This study investigates these spatial effects and their determinants across three distinct administrative levels within Hubei Province, China. To analyze the spatial patterns and heterogeneity of multi-scale regional poverty, we employed various spatial analysis techniques, including the global and local Moran’s I statistics, the Lineman, Merenda, and Gold (LMG) method, as well as Multiscale Geographically Weighted Regression (MGWR). We found that: (1) Regional poverty exhibits significant spatial dependence across various scales, with a higher level of spatial dependence observed at higher administrative levels. (2) The spatial distribution of poverty is primarily influenced by geographical factors, encompassing first-, second-, and third-nature geographical elements. Notably, first-nature geographical factors make substantial contributions, accounting for 36.99%, 42.23%, and 23.79% at the county, township, and village levels, respectively. (3) The influence of geographical factors varies with scale. Global effects of various factors may transcend scales or remain confined to specific scales, while the local impacts of different factors also exhibit variations across scales. These results underscore the necessity for collaborative efforts among government entities at different levels with the anti-poverty measures tailored to local contexts.
LcSAO1, an Unconventional DOXB Clade 2OGD Enzyme from Ligusticum chuanxiong Catalyzes the Biosynthesis of Plant-Derived Natural Medicine Butylphthalide
Authors: Chen Xueqing , Zhang Xiaopeng , Sun Wenkai , Hou Zhuangwei , Nie Bao , Wang Fengjiao , Yang Song , Feng Shourui , Li Wei , Wang Li
Published: December 2023

Butylphthalide, a prescription medicine recognized for its efficacy in treating ischemic strokes approved by the State Food and Drug Administration of China in 2005, is sourced from the traditional botanical remedy Ligusticum chuanxiong. While chemical synthesis offers a viable route, limitations in the production of isomeric variants with compromised bioactivity necessitate alternative strategies. Addressing this issue, biosynthesis offers a promising solution. However, the intricate in vivo pathway for butylphthalide biosynthesis remains elusive. In this study, we examined the distribution of butylphthalide across various tissues of L. chuanxiong and found a significant accumulation in the rhizome. By searching transcriptome data from different tissues of L. chuanxiong, we identified four rhizome-specific genes annotated as 2-oxoglutarate-dependent dioxygenase (2-OGDs) that emerged as promising candidates involved in butylphthalide biosynthesis. Among them, LcSAO1 demonstrates the ability to catalyze the desaturation of senkyunolide A at the C-4 and C-5 positions, yielding the production of butylphthalide. Experimental validation through transient expression assays in Nicotiana benthamiana corroborates this transformative enzymatic activity. Notably, phylogenetic analysis of LcSAO1 revealed that it belongs to the DOXB clade, which typically encompasses genes with hydroxylation activity, rather than desaturation. Further structure modelling and site-directed mutagenesis highlighted the critical roles of three amino acid residues, T98, S176, and T178, in substrate binding and enzyme activity. By unraveling the intricacies of the senkyunolide A desaturase, the penultimate step in the butylphthalide biosynthesis cascade, our findings illuminate novel avenues for advancing synthetic biology research in the realm of medicinal natural products.
The Role of Tumor Metabolic Reprogramming in Tumor Immunity
Authors: Zhang Xianhong , Song Weiguo , Gao Yue , Zhang Yu , Zhao Yuqi , Hao Shuailin , Ni Ting
Published: December 2023

The occurrence and development of tumors require the metabolic reprogramming of cancer cells, namely the alteration of flux in an autonomous manner via various metabolic pathways to meet increased bioenergetic and biosynthetic demands. Tumor cells consume large quantities of nutrients and produce related metabolites via their metabolism; this leads to the remodeling of the tumor microenvironment (TME) to better support tumor growth. During TME remodeling, the immune cell metabolism and antitumor immune activity are affected. This further leads to the escape of tumor cells from immune surveillance and therefore to abnormal proliferation. This review summarizes the regulatory functions associated with the abnormal biosynthesis and activity of metabolic signaling molecules during the process of tumor metabolic reprogramming. In addition, we provide a comprehensive description of the competition between immune cells and tumor cells for nutrients in the TME, as well as the metabolites required for tumor metabolism, the metabolic signaling pathways involved, and the functionality of the immune cells. Finally, we summarize current research targeted at the development of tumor immunotherapy. We aim to provide new concepts for future investigations of the mechanisms underlying the metabolic reprogramming of tumors and explore the association of these mechanisms with tumor immunity.
High-Throughput Transcriptomics Differentiates Toxic versus Non-Toxic Chemical Exposures Using a Rat Liver Model
Authors: Pannala Venkat , Balik-Meisner Michele , Mav Deepak , Phadke Dhiral , Scholl Elizabeth , Shah Ruchir , Auerbach Scott , Wallqvist Anders
Published: December 2023

To address the challenge of limited throughput with traditional toxicity testing, a newly developed high-throughput transcriptomics (HTT) platform, together with a 5-day in vivo rat model, offers an alternative approach to estimate chemical exposures and provide reasonable estimates of toxicological endpoints. This study contains an HTT analysis of 18 environmental chemicals with known liver toxicity. They were evaluated using male Sprague Dawley rats exposed to various concentrations daily for five consecutive days via oral gavage, with data collected on the sixth day. Here, we further explored the 5-day rat model to identify potential gene signatures that can differentiate between toxic and non-toxic liver responses and provide us with a potential histopathological endpoint of chemical exposure. We identified a distinct gene expression pattern that differentiated non-hepatotoxic compounds from hepatotoxic compounds in a dose-dependent manner, and an analysis of the significantly altered common genes indicated that toxic chemicals predominantly upregulated most of the genes and several pathways in amino acid and lipid metabolism. Finally, our liver injury module analysis revealed that several liver-toxic compounds showed similarities in the key injury phenotypes of cellular inflammation and proliferation, indicating potential molecular initiating processes that may lead to a specific end-stage liver disease.
Mid-Regional Pro-Adrenomedullin Can Predict Organ Failure and Prognosis in Sepsis?
Authors: Spoto Silvia , Basili Stefania , Cangemi Roberto , D’Avanzo Giorgio , Lupoi Domenica Marika , Romiti Giulio Francesco , Argemi Josepmaria , Yuste José Ramón , Lucena Felipe , Locorriere Luciana , Masini Francesco , Testorio Giulia , Calarco Rodolfo , Fogolari Marta , Francesconi Maria , Battifoglia Giulia , Costantino Sebastiano , Angeletti Silvia
Published: December 2023

Sepsis causes immune dysregulation and endotheliitis, with an increase in mid-regional pro-adrenomedullin (MR-proADM). The aim of the study is to determine an MR-proADM value that, in addition to clinical diagnosis, can identify patients with localized infection or those with sepsis/septic shock, with specific organ damage or with the need for intensive care unit (ICU) transfer and prognosis. The secondary aim is to correlate the MR-proADM value with the length of stay (LOS). In total, 301 subjects with sepsis (124/301 with septic shock) and 126 with localized infection were retrospectively included. In sepsis, MR-proADM ≥ 3.39 ng/mL identified acute kidney injury (AKI); ≥2.99 ng/mL acute respiratory distress syndrome (ARDS); ≥2.28 ng/mL acute heart failure (AHF); ≥2.55 ng/mL Glascow Coma Scale (GCS) < 15; ≥3.38 multi-organ involvement; ≥3.33 need for ICU transfer; ≥2.0 Sequential Organ Failure Assessment (SOFA) score ≥ 2; and ≥3.15 ng/mL non-survivors. The multivariate analysis showed that MR-proADM ≥ 2 ng/mL correlates with AKI, anemia and SOFA score ≥ 2, and MR-proADM ≥ 3 ng/mL correlates with AKI, GCS < 15 and SOFA score ≥ 2. A correlation between mortality and AKI, GCS < 15, ICU transfer and cathecolamine administration was found. In localized infection, MR-proADM at admission ≥ 1.44 ng/mL identified patients with AKI; ≥1.0 ng/mL with AHF; and ≥1.44 ng/mL with anemia and SOFA score ≥ 2. In the multivariate analysis, MR-proADM ≥ 1.44 ng/mL correlated with AKI, anemia, SOFA score ≥ 2 and AHF. MR-proADM is a marker of oxidative stress due to an infection, reflecting severity proportionally to organ damage.
Protective Effect and Mechanism of Aspirin Eugenol Ester on Lipopolysaccharide-Induced Intestinal Barrier Injury
Authors: Tao Qi , Liu Xi-Wang , Zhang Zhen-Dong , Ma Ning , Lu Xiao-Rong , Ge Wen-Bo , Li Jian-Yong , Yang Ya-Jun
Published: December 2023

Intestinal inflammation is a complex and recurrent inflammatory disease. Pharmacological and pharmacodynamic experiments showed that aspirin eugenol ester (AEE) has good anti-inflammatory, antipyretic, and analgesic effects. However, the role of AEE in regulating intestinal inflammation has not been explored. This study aimed to investigate whether AEE could have a protective effect on LPS-induced intestinal inflammation and thus help to alleviate the damage to the intestinal barrier. This was assessed with an inflammation model in Caco-2 cells and in rats induced with LPS. The expression of inflammatory mediators, intestinal epithelial barrier-related proteins, and redox-related signals was analyzed using an enzyme-linked immunosorbent assay (ELISA), Western blotting, immunofluorescence staining, and RT-qPCR. Intestinal damage was assessed by histopathological examination. Changes in rat gut microbiota and their functions were detected by the gut microbial metagenome. AEE significantly reduced LPS-induced pro-inflammatory cytokine levels (p < 0.05) and oxidative stress levels in Caco-2 cells and rats. Compared with the LPS group, AEE could increase the relative expression of Occludin, Claudin-1, and zonula occludens-1 (ZO-1) and decrease the relative expression of kappa-B (NF-κB) and matrix metalloproteinase-9. AEE could significantly improve weight loss, diarrhea, reduced intestinal muscle thickness, and intestinal villi damage in rats. Metagenome results showed that AEE could regulate the homeostasis of the gut flora and alter the relative abundance of Firmicutes and Bacteroidetes. Flora enrichment analysis indicated that the regulation of gut flora with AEE may be related to the regulation of glucose metabolism and energy metabolism. AEE could have positive effects on intestinal inflammation-related diseases.
Optimization of Sirius Red-Based Microplate Assay to Investigate Collagen Production In Vitro
Authors: Szász Csenge , Pap Domonkos , Szebeni Beáta , Bokrossy Péter , Őrfi László , Szabó Attila J. , Vannay Ádám , Veres-Székely Apor
Published: December 2023

Tissue fibrosis is characterized by chronic fibroblast activation and consequently excessive accumulation of collagen-rich extracellular matrix. In vitro microplate-based assays are essential to investigate the underlying mechanism and the effect of antifibrotic drugs. In this study, in the absence of a gold-standard method, we optimized a simple, cost-effective, Sirius Red-based colorimetric measurement to determine the collagen production of fibroblasts grown on 96-well tissue culture plates. Based on our findings, the use of a serum-free medium is recommended to avoid aspecific signals, while ascorbate supplementation increases the collagen production of fibroblasts. The cell-associated collagens can be quantified by Sirius Red staining in acidic conditions followed by alkaline elution. Immature collagens can be precipitated from the culture medium by acidic Sirius Red solution, and after subsequent centrifugation and washing steps, their amount can be also measured. Increased attention has been paid to optimizing the assay procedure, including incubation time, temperature, and solution concentrations. The resulting assay shows high linearity and sensitivity and could serve as a useful tool in fibrosis-related basic research as well as in preclinical drug screening.
Granulocyte-Macrophage-Colony-Stimulating-Factor Combined with Prostaglandin E1 Create Dendritic Cells of Leukemic Origin from AML Patients’ Whole Blood and Whole Bone Marrow That Mediate Antileukemic Processes after Mixed Lymphocyte Culture
Authors: Unterfrauner Marianne , Rejeski Hazal Aslan , Hartz Anne , Bohlscheid Sophia , Baudrexler Tobias , Feng Xiaojia , Rackl Elias , Li Lin , Rank Andreas , Filippini Velázquez Giuliano , Schmid Christoph , Schmohl Jörg , Bojko Peter , Schmetzer Helga
Published: December 2023

Although several (chemotherapeutic) protocols to treat acute myeloid leukemia (AML) are available, high rates of relapses in successfully treated patients occur. Strategies to stabilize remissions are greatly needed. The combination of the (clinically approved) immune-modulatory compounds Granulocyte-Macrophage-Colony-Stimulating-Factor (GM-CSF) and Prostaglandine E1 (PGE-1) (Kit-M) converts myeloid blasts into dendritic cells of leukemic origin (DCleu). After stimulation with DCleu ex vivo, leukemia-specific antileukemic immune cells are activated. Therefore, Kit-M treatment may be an attractive immunotherapeutic tool to treat patients with myeloid leukemia. Kit-M-mediated antileukemic effects on whole bone marrow (WBM) were evaluated and compared to whole blood (WB) to evaluate the potential effects of Kit-M on both compartments. WB and WBM samples from 17 AML patients at first diagnosis, in persisting disease and at relapse after allogeneic stem cell transplantation (SCT) were treated in parallel with Kit-M to generate DC/DCleu. Untreated samples served as controls. After a mixed lymphocyte culture enriched with patients’ T cells (MLC), the leukemia-specific antileukemic effects were assessed through the degranulation- (CD107a+ T cells), the intracellular IFNγ production- and the cytotoxicity fluorolysis assay. Quantification of cell subtypes was performed via flow cytometry. In both WB and WBM significantly higher frequencies of (mature) DCleu were generated without induction of blast proliferation in Kit-M-treated samples compared to control. After MLC with Kit-M-treated vs. not pretreated WB or WBM, frequencies of (leukemia-specific) immunoreactive cells (e.g., non-naive, effector-, memory-, CD3+β7+ T cells, NK- cells) were (significantly) increased, whereas leukemia-specific regulatory T cells (Treg, CD152+ T cells) were (significantly) decreased. The cytotoxicity fluorolysis assay showed a significantly improved blast lysis in Kit-M-treated WB and WBM compared to control. A parallel comparison of WB and WBM samples revealed no significant differences in frequencies of DCleu, (leukemia-specific) immunoreactive cells and achieved antileukemic processes. Kit-M was shown to have comparable effects on WB and WBM samples regarding the generation of DCleu and activation of (antileukemic) immune cells after MLC. This was true for samples before or after SCT. In summary, a potential Kit-M in vivo treatment could lead to antileukemic effects in WB as well as WBM in vivo and to stabilization of the disease or remission in patients before or after SCT. A clinical trial is currently being planned.
Serum Metabolomic Profiling of Patients with Lipedema
Authors: Kempa Sally , Buechler Christa , Föh Bandik , Felthaus Oliver , Prantl Lukas , Günther Ulrich L. , Müller Martina , Derer-Petersen Stefanie , Sina Christian , Schmelter Franziska , Tews Hauke C.
Published: December 2023

Lipedema is a chronic condition characterized by disproportionate and symmetrical enlargement of adipose tissue, predominantly affecting the lower limbs of women. This study investigated the use of metabolomics in lipedema research, with the objective of identifying complex metabolic disturbances and potential biomarkers for early detection, prognosis, and treatment strategies. The study group (n = 25) comprised women diagnosed with lipedema. The controls were 25 lean women and 25 obese females, both matched for age. In the patients with lipedema, there were notable changes in the metabolite parameters. Specifically, lower levels of histidine and phenylalanine were observed, whereas pyruvic acid was elevated compared with the weight controls. The receiver operating characteristic (ROC) curves for the diagnostic accuracy of histidine, phenylalanine, and pyruvic acid concentrations in distinguishing between patients with lipedema and those with obesity but without lipedema revealed good diagnostic ability for all parameters, with pyruvic acid being the most promising (area under the curve (AUC): 0.9992). Subgroup analysis within matched body mass index (BMI) ranges (30.0 to 39.9 kg/m2) further revealed that differences in pyruvic acid, phenylalanine, and histidine levels are likely linked to lipedema pathology rather than BMI variations. Changes in low-density lipoprotein (LDL)-6 TG levels and significant reductions in various LDL-2-carried lipids of patients with lipedema, compared with the lean controls, were observed. However, these lipids were similar between the lipedema patients and the obese controls, suggesting that these alterations are related to adiposity. Metabolomics is a valuable tool for investigating lipedema, offering a comprehensive view of metabolic changes and insights into lipedema’s underlying mechanisms.
Sex, Endothelial Cell Functions, and Peripheral Artery Disease
Authors: Cartland Siân P. , Stanley Christopher P. , Bursill Christina , Passam Freda , Figtree Gemma A. , Patel Sanjay , Loa Jacky , Golledge Jonathan , Robinson David A. , Aitken Sarah J. , Kavurma Mary M.
Published: December 2023

Peripheral artery disease (PAD) is caused by blocked arteries due to atherosclerosis and/or thrombosis which reduce blood flow to the lower limbs. It results in major morbidity, including ischemic limb, claudication, and amputation, with patients also suffering a heightened risk of heart attack, stroke, and death. Recent studies suggest women have a higher prevalence of PAD than men, and with worse outcomes after intervention. In addition to a potential unconscious bias faced by women with PAD in the health system, with underdiagnosis, and lower rates of guideline-based therapy, fundamental biological differences between men and women may be important. In this review, we highlight sexual dimorphisms in endothelial cell functions and how they may impact PAD pathophysiology in women. Understanding sex-specific mechanisms in PAD is essential for the development of new therapies and personalized care for patients with PAD.
Maackiain Mimics Caloric Restriction through aak-2-Mediated Lipid Reduction in Caenorhabditis elegans
Authors: Mladenova Saveta G. , Todorova Monika N. , Savova Martina S. , Georgiev Milen I. , Mihaylova Liliya V.
Published: December 2023

Obesity prevalence is becoming a serious global health and economic issue and is a major risk factor for concomitant diseases that worsen the quality and duration of life. Therefore, the urgency of the development of novel therapies is of a particular importance. A previous study of ours revealed that the natural pterocarpan, maackiain (MACK), significantly inhibits adipogenic differentiation in human adipocytes through a peroxisome proliferator-activated receptor gamma (PPARγ)-dependent mechanism. Considering the observed anti-adipogenic potential of MACK, we aimed to further elucidate the molecular mechanisms that drive its biological activity in a Caenorhabditis elegans obesity model. Therefore, in the current study, the anti-obesogenic effect of MACK (25, 50, and 100 μM) was compared to orlistat (ORST, 12 μM) as a reference drug. Additionally, the hybrid combination between the ORST (12 μM) and MACK (100 μM) was assessed for suspected synergistic interaction. Mechanistically, the observed anti-obesogenic effect of MACK was mediated through the upregulation of the key metabolic regulators, namely, the nuclear hormone receptor 49 (nhr-49) that is a functional homologue of the mammalian PPARs and the AMP-activated protein kinase (aak-2/AMPK) in C. elegans. Collectively, our investigation indicates that MACK has the potential to limit lipid accumulation and control obesity that deserves future developments.
MiR-221-3p/222-3p Cluster Expression in Human Adipose Tissue Is Related to Obesity and Type 2 Diabetes
Authors: Gentile Adriana-Mariel , Lhamyani Said , Mengual-Mesa María , García-Fuentes Eduardo , Bermúdez-Silva Francisco-Javier , Rojo-Martínez Gemma , Clemente-Postigo Mercedes , Rodriguez-Cañete Alberto , Olveira Gabriel , El Bekay Rajaa
Published: December 2023

The progression of obesity and type 2 diabetes (T2D) is intricately linked with adipose tissue (AT) angiogenesis. Despite an established network of microRNAs (miRNAs) regulating AT function, the specific role of angiogenic miRNAs remains less understood. The miR-221/222 cluster has recently emerged as being associated with antiangiogenic activity. However, no studies have explored its role in human AT amidst the concurrent development of obesity and T2D. Therefore, this study aims to investigate the association between the miR-221-3p/222-3p cluster in human AT and its regulatory network with obesity and T2D. MiR-221-3p/222-3p and their target gene (TG) expression levels were quantified through qPCR in visceral (VAT) and subcutaneous (SAT) AT from patients (n = 33) categorized based on BMI as normoweight (NW) and obese (OB) and by glycemic status as normoglycemic (NG) and type 2 diabetic (T2D) subjects. In silico analyses of miR-221-3p/222-3p and their TGs were conducted to identify pertinent signaling pathways. The results of a multivariate analysis, considering the simultaneous expression of miR-221-3p and miR-222-3p as dependent variables, revealed statistically significant distinctions when accounting for variables such as tissue depot, obesity, sex, and T2D as independent factors. Furthermore, both miRNAs and their TGs exhibited differential expression patterns based on obesity severity, glycemic status, sex, and type of AT depot. Our in silico analysis indicated that miR-221-3p/222-3p cluster TGs predominantly participate in angiogenesis, WNT signaling, and apoptosis pathways. In conclusion, these findings underscore a promising avenue for future research, emphasizing the miR-221-3p/222-3p cluster and its associated regulatory networks as potential targets for addressing obesity and related metabolic disorders.
Sentiment Analysis in the Age of COVID-19: A Bibliometric Perspective
Authors: Sandu Andra , Cotfas Liviu-Adrian , Delcea Camelia , Crăciun Liliana , Molănescu Anca Gabriela
Published: December 2023

The global impact of the COVID-19 pandemic has been profound, placing significant challenges upon healthcare systems and the world economy. The pervasive presence of illness, uncertainty, and fear has markedly diminished overall life satisfaction. Consequently, sentiment analysis has gained substantial traction among scholars seeking to unravel the emotional and attitudinal dimensions of this crisis. This research endeavors to provide a bibliometric perspective, shedding light on the principal contributors to this emerging field. It seeks to spotlight the academic institutions associated with this research domain, along with identifying the most influential publications in terms of both paper volume and h-index metrics. To this end, we have meticulously curated a dataset comprising 646 papers sourced from the ISI Web of Science database, all centering on the theme of sentiment analysis during the COVID-19 pandemic. Our findings underscore a burgeoning interest exhibited by the academic community in this particular domain, evident in an astonishing annual growth rate of 153.49%. Furthermore, our analysis elucidates key keywords and collaborative networks within the authorship, offering valuable insights into the global proliferation of this thematic pursuit. In addition to this, our analysis encompasses an n-gram investigation across keywords, abstracts, titles, and keyword plus, complemented by an examination of the most frequently cited works. The results gleaned from these endeavors offer crucial perspectives, contribute to the identification of pertinent issues, and provide guidance for informed decision-making.
Phylogeography of Two Enigmatic Sulphur Butterflies, Colias mongola Alphéraky, 1897 and Colias tamerlana Staudinger, 1897 (Lepidoptera, Pieridae), with Relations to Wolbachia Infection
Authors: Shapoval Nazar A. , Kir’yanov Alexander V. , Krupitsky Anatoly V. , Yakovlev Roman V. , Romanovich Anna E. , Zhang Jing , Cong Qian , Grishin Nick V. , Kovalenko Margarita G. , Shapoval Galina N.
Published: December 2023

The genus Colias Fabricius, 1807 includes numerous taxa and forms with uncertain status and taxonomic position. Among such taxa are Colias mongola Alphéraky, 1897 and Colias tamerlana Staudinger, 1897, interpreted in the literature either as conspecific forms, as subspecies of different but morphologically somewhat similar Colias species or as distinct species-level taxa. Based on mitochondrial and nuclear DNA markers, we reconstructed a phylogeographic pattern of the taxa in question. We recover and include in our analysis DNA barcodes of the century-old type specimens, the lectotype of C. tamerlana deposited in the Natural History Museum (Museum für Naturkunde), Berlin, Germany (ZMHU) and the paralectotype of C. tamerlana and the lectotype of C. mongola deposited in the Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia (ZISP). Our analysis grouped all specimens within four (HP_I–HP_IV) deeply divergent but geographically poorly structured clades which did not support nonconspecifity of C. mongola–C. tamerlana. We also show that all studied females of the widely distributed haplogroup HP_II were infected with a single Wolbachia strain belonging to the supergroup B, while the males of this haplogroup, as well as all other investigated specimens of both sexes, were not infected. Our data highlight the relevance of large-scale sampling dataset analysis and the need for testing for Wolbachia infection to avoid erroneous phylogenetic reconstructions and species misidentification.
Conometric Connection for Implant-Supported Crowns: A Prospective Clinical Cohort Study
Authors: Lupi Saturnino Marco , De Martis Dario , Todaro Claudia , Isola Gaetano , Beretta Mario , Rodriguez y Baena Ruggero
Published: December 2023

Background: Traditional screw or cemented connections in dental implants present limitations, prompting the exploration of alternative methods. This study assesses the clinical outcomes of single crowns and fixed partial prostheses supported by conometric connections after one year of follow-up. Methods: Twenty-two patients received 70 implants, supporting 33 rehabilitations. Biological responses and prosthodontic complications were evaluated at baseline, 6 months, and 12 months. Results: All implants exhibited successful osseointegration, with no losses or peri-implant inflammation. Marginal bone levels showed minimal changes, well below pathological thresholds. The difference in marginal bone loss (MBL) was −0.27 ± 0.79 mm between T0 and T1, and −0.51 ± 0.93 mm between T0 and T2. No abutment screw loosening or crown chipping occurred. However, coupling stability loss was observed in nine cases. Conclusions: The conometric connection demonstrated successful integration and minimal complications after one year. This alternative shows promise, particularly in simplifying handling and improving marginal adaptation. Further research with larger sample sizes and longer follow-up is warranted for comprehensive validation.
ViTSTR-Transducer: Cross-Attention-Free Vision Transformer Transducer for Scene Text Recognition
Authors: Buoy Rina , Iwamura Masakazu , Srun Sovila , Kise Koichi
Published: December 2023

Attention-based encoder–decoder scene text recognition (STR) architectures have been proven effective in recognizing text in the real world, thanks to their ability to learn an internal language model. Nevertheless, the cross-attention operation that is used to align visual and linguistic features during decoding is computationally expensive, especially in low-resource environments. To address this bottleneck, we propose a cross-attention-free STR framework that still learns a language model. The framework we propose is ViTSTR-Transducer, which draws inspiration from ViTSTR, a vision transformer (ViT)-based method designed for STR and the recurrent neural network transducer (RNN-T) initially introduced for speech recognition. The experimental results show that our ViTSTR-Transducer models outperform the baseline attention-based models in terms of the required decoding floating point operations (FLOPs) and latency while achieving a comparable level of recognition accuracy. Compared with the baseline context-free ViTSTR models, our proposed models achieve superior recognition accuracy. Furthermore, compared with the recent state-of-the-art (SOTA) methods, our proposed models deliver competitive results.
A Method for Predicting the Load Interaction between Reinforced Thermoplastic Pipe and Sandy Soil Based on Model Testing
Authors: Wang Chuan , Liu Lianghai , Zhang Ya , Lou Min
Published: December 2023

This study aims to investigate the interaction between reinforced thermoplastic pipes (RTPs) and sandy soil. The mechanical properties of sandy soil in the South China Sea region were determined through shear tests to obtain fundamental data. Subsequently, a specialized experimental setup was designed and assembled to study the pipe–soil interaction, specifically measuring the lateral soil resistance of flexible pipes at varying burial depths. Data analysis revealed the relationship between soil resistance, lateral displacement, and initial burial depth. To simulate the mechanical behavior of the pipe–soil interaction, the coupled Eulerian–Lagrangian (CEL) method was employed for numerical simulations. The research findings indicate that the lateral soil resistance is influenced by the uplift height and accumulation width of the soil ahead of the pipe. Within a lateral displacement range of 0.5 times the pipe diameter (0.5D), the lateral soil resistance rapidly increases, resulting in a soil uplift along the circumferential direction of the pipe. This process not only enhances the load-bearing capacity of the pipe but also increases the accumulated soil resistance, consequently expanding the soil failure zone. Furthermore, the ultimate soil resistance exhibits an increasing trend with an increasing burial depth. Once the pipe reaches a certain burial depth, the uplift height of the soil reaches a critical state. To address the grid distortion caused by soil deformation, numerical simulations based on the CEL method effectively modeled the pipe–soil interaction forces under significant lateral displacements, exhibiting good agreement with the experimental results. This study provides a solution for investigating soil resistance in submarine pipelines, thereby contributing significantly to the design and performance prediction of underwater pipelines.
The Lateral Behavior of Large-Diameter Monopiles for Offshore Wind Turbines Based on the p-y Curve and Solid FEM Methods
Authors: Li Tao , Yu Xinran , He Ben , Dai Song
Published: December 2023

With the rapid increase in offshore wind turbines in China, monopiles with diameters exceeding 2 m are widely used. As these piles are subjected to lateral loads caused by wind, waves, and currents, the designs of the pile foundations supporting the offshore wind turbines are significantly influenced by their lateral behaviors. For this reason, field tests of the largest monopile on the sea and additional analysis based on the solid finite element method (FEM) and p-y curves are carried out to reveal the response of monopiles subjected to lateral loads and to figure out key technical issues related to the design process. The results revealed that the p-y curves proposed by the API code for clay showed a much “softer” response, which resulted in the conservative design of the piles. The solid FEM relied heavily on the choosing of the parameters used. At relatively small deflections, the solid FEM presented reasonable results as compared with the tests which were, however, supposed to overestimate the ultimate capacity of the piles. The results also indicated the importance of the influence of the pile–soil gap and the application of parameter analysis to achieve relatively conservative results, if the solid FEM is adopted in the design.
Correction: Silva-Filho et al. Eoscyphella luciurceolata gen. and sp. nov. (Agaricomycetes) Shed Light on Cyphellopsidaceae with a New Lineage of Bioluminescent Fungi. J. Fungi 2023, 9, 1004
Authors: Silva-Filho Alexandre G. S. , Mombert Andgelo , Nascimento Cristiano C. , Nóbrega Bianca B. , Soares Douglas M. M. , Martins Ana G. S. , Domingos Adão H. R. , Santos Isaias , Della-Torre Olavo H. P. , Perry Brian A. , Desjardin Dennis E. , Stevani Cassius V. , Menolli Nelson
Published: December 2023

In the original publication [...]
Diversity and Advantages of Culturable Endophytic Fungi from Tea (Camellia sinensis)
Authors: Onlamun Thanyarat , Boonthavee Autchima , Brooks Siraprapa
Published: December 2023

Sordariomycetes, Dothideomycetes, and Eurotioycetes are three classes of endophytes that colocalize with tea (Camellia sinensis). Overall, the diversity indexes in this study indicated a greater abundance of fungal endophytes in roots and stems. Taking the production system into account, conventional tea plantations exhibit lower diversity compared to organic tea plantations. Notably, the influence of agrochemicals had the largest impact on the fungal endophyte communities within roots and young leaves. Despite the limited fungal diversity in conventional plantations, three fungal endophytes were isolated from tea in this culture system: Diaporthe sp., YI-005; Diaporthe sp., SI-007; and Eurotium sp., RI-008. These isolated endophytes exhibited high antagonistic activity (93.00–97.00% inhibition of hypha growth) against Stagonosporopsis cucurbitacearum, the causal agent of gummy stem blight disease. On the other hand, endophytic fungi isolated from tea in an organic system—Pleosporales sp., SO-006 and Pleosporales sp., RO-013—established the ability to produce indole-3-acetic acid (IAA; 0.65 ± 0.06 µg/mL) and assist the solubilizing phosphorus (5.17 ± 1.03 µg/mL) from the soil, respectively. This suggested that the level of diversity, whether at the tissue level or within the farming system, did not directly correlate with the discovery of beneficial fungi. More importantly, these beneficial fungi showed the potential to develop into biological agents to control the devastating diseases in the cucurbit family and the potential for use as biofertilizers with a wide range of applications in plants. Therefore, it can be concluded that there are no restrictions limiting the use of fungal endophytes solely to the plant host from which they were originally isolated.
Clinical Implementation of β-Tubulin Gene-Based Aspergillus Polymerase Chain Reaction for Enhanced Aspergillus Diagnosis in Patients with Hematologic Diseases: A Prospective Observational Study
Authors: Lee Raeseok , Kim Won-Bok , Cho Sung-Yeon , Nho Dukhee , Park Chulmin , Yoo In Young , Park Yeon-Joon , Lee Dong-Gun
Published: December 2023

The β-tubulin (benA) gene is a promising target for the identification of Aspergillus species. Assessment of the clinical implementation and performance of benA gene-based Aspergillus polymerase chain reaction (PCR) remains warranted. In this study, we assessed the analytical performance of the BenA probe PCR in comparison with the Aspergenius kit. We prospectively collected bronchoalveolar lavage (BAL) fluid via diagnostic bronchoscopy from adult patients with hematologic diseases. BenA gene-based multiplex real-time PCR and sequential melting temperature analysis were performed to detect the azole resistance of Aspergillus fumigatus. In total, 76 BAL fluids in 75 patients suspicious of invasive pulmonary aspergillosis (IPA) were collected. Before the application of PCR, the prevalence of proven and probable IPA was 32.9%. However, after implementing the benA gene-based PCR, 15.8% (12 out of 76) of potential IPA cases were reclassified as probable IPA. The analytical performance of the BenA probe PCR in BAL samples was comparable to that of the Aspergenius kit. The diagnostic performance was as follows: sensitivity, 52.0%; specificity, 64.7%; positive predictive value, 41.9%; negative predictive value, 73.3%; positive likelihood ratio, 1.473; and negative likelihood ratio, 0.741. Moreover, benA gene-based Aspergillus PCR discriminated all major sections of Aspergillus, including cryptic species such as Aspergillus tubingensis. Sequential melting temperature analysis successfully detected 2 isolates (15.4%) of A. fumigatus carrying resistant mutations. BenA gene-based Aspergillus PCR with melting temperature analysis enhances diagnostic accuracy and detects not only cryptic species but also resistant mutations of A. fumigatus. It shows promise for clinical applications in the diagnosis of IPA.
Altruism in eWOM: Propensity to Write Reviews on Hotel Experience
Authors: Llorens-Marin Miguel , Hernandez Adolfo , Puelles-Gallo Maria
Published: December 2023

This research tests the relationship between aspects of customer influenceability at the time of booking a hotel with the propensity to write a review in electronic word-of-mouth communication. A valid sample of 739 online questionnaires was obtained. An Exploratory Factor Analysis was conducted in order to reduce the dimensions of the two critical variables, and a measurement model was built. Then a Path analysis was carried out. The novelty of this research lies in measuring the evolution from being a passive eWOM reader to a proactive eWOM writer. Results indicate a relationship between being influenced by reading reviews and the propensity to write reviews. The most important underlying motivation to write a review is altruistic. Managers should try to identify the most responsive customers and encourage them to write reviews on altruistic grounds. This study effectively validated the impact of being responsive to reading reviews on the inclination to, in turn, write them. Findings contribute to the evolving research landscape in eWOM within the hospitality and tourism sector, offering practical insights for industry practitioners to formulate more effective strategies in soliciting and managing customer reviews.
Sodium–Glucose Transporter 2 (SGLT2) Inhibitors and Iron Deficiency in Heart Failure and Chronic Kidney Disease: A Literature Review
Authors: Tziastoudi Maria , Pissas Georgios , Golfinopoulos Spyridon , Filippidis Georgios , Dousdampanis Periklis , Eleftheriadis Theodoros , Stefanidis Ioannis
Published: December 2023

Heart failure (HF) and chronic kidney disease (CKD) are associated with high mortality. In both disorders, impaired iron homeostasis, mostly in the form of a functional iron deficiency, is a frequent co-morbidity. In HF, functional iron deficiency and management by i.v. iron supplementation have been proven to affect both prognosis and functional capacity. In the same context, iron supplementation is routine for the adequate management of renal anemia in CKD. In numerous recent studies in HF and in CKD, sodium–glucose transporter 2 (SGLT2) inhibitor treatment has been proven to significantly reduce mortality. Furthermore, the same trials showed that these drugs alleviate iron deficiency and anemia. These effects of SGLT2 inhibitors may be due to an amelioration of inflammation with reduced interleukin-6 (IL-6) and to an enhancement of autophagy with increased sirtuin 1 (SIRT1), both associated with modified production of hepcidin and enhanced ferritinophagy. However, the exact pathogenic basis of the beneficial SGLT2 inhibitor action is not fully elucidated. Nevertheless, effects on iron homeostasis might be a potential explanatory mechanism for the powerful SGLT2 inhibitors’ cardiovascular and renal outcome benefits. In addition, the interaction between iron supplementation and SGLT2 inhibitors and its potential impact on prognosis remains to be clarified by future studies. This review represents a significant effort to explore the complex relationships involved, seeking to elucidate the intricate mechanisms by which SGLT2 inhibitors influence iron homeostasis.
3-Benzoyl-2-hydroxy-3a-[(3-methylquinoxalin-2-yl)methyl]-1H-pyrrolo[2,1-c][1,4]benzothiazine-1,4(3aH)-dione
Authors: Lystsova Ekaterina A. , Khramtsova Ekaterina E.
Published: December 2023

The reaction of 3-benzoylpyrrolo[2,1-c][1,4]benzothiazine-1,2,4-trione with 2,3-dimethylquinoxaline afforded 3-benzoyl-2-hydroxy-3a-[(3-methylquinoxalin-2-yl)methyl]-1H-pyrrolo[2,1-c][1,4]benzothiazine-1,4(3aH)-dione in a moderate yield. The compound was fully characterized.
A Design Method for Rectangular Waveguide-Typed Microwave Devices Based on a Novel Origami Process
Authors: Sun Yipeng , Gao Chuyuan , Chen Lijun , Han Lei
Published: December 2023

A novel design method based on a novel origami process that can create a solid structure swiftly and at a low cost is presented for rectangular waveguide-type microwave devices in this paper. A planar structure was fabricated by the lamination and laser cutting of polystyrene membranes and aluminum foils and was converted into a solid structure via origami in accordance with the selective absorption of infrared light. A rectangular waveguide, a rectangular waveguide-type coupler, and a power divider based on an origami structure with a multi-layer structure and a single-layer structure were fabricated and tested, demonstrating easy assembly and good microwave performance. The measured results of the rectangular waveguide indicated that the insertion loss was superior to −0.9 dB. Meanwhile, the results of the coupler showed that the coupling degree increased from −12.8 dB to −8.9 dB in the range of 11.0 GHz to 12.0 GHz. Correspondingly, the prepared power divider demonstrated that the return loss dwindled from −8.9 dB to −11.3 dB and that the insertion loss of one output port was approximate to that of the remaining one, varying between −3.5 dB and −5.2 dB in the range of 10.5 GHz to 11.5 GHz—verifying the effectiveness of the origami-based design method.
Metal Organic Frameworks: Current State and Analysis of Their Use as Modifiers of the Vulcanization Process and Properties of Rubber
Authors: Klajn Katarzyna , Gozdek Tomasz , Bieliński Dariusz M.
Published: December 2023

The interest in and application of metal organic frameworks (MOF) is increasing every year. These substances are widely used in many places, including the separation and storage of gases and energy, catalysis, electrochemistry, optoelectronics, and medicine. Their use in polymer technology is also increasing, focusing mainly on the synthesis of MOF-polymer hybrid compounds. Due to the presence of metal ions in their structure, they can also serve as a component of the crosslinking system used for curing elastomers. This article presents the possibility of using zeolitic imidazolate framework ZIF-8 or MOF-5 as activators for sulfur vulcanization of styrene-butadiene rubber (SBR), replacing zinc oxide in conventional (CV) or effective (EF) curing systems to different extents. Their participation in the curing process and influence on the crosslinking density and structure, as well as the mechanical and thermal properties of the rubber vulcanizates, were examined.
Effect of Nb Content and Second Heat Cycle Peak Temperatures on Toughness of X80 Pipeline Steel
Authors: Chen Yuefeng , Yang Yaobin , He Xiaodong , Chi Qiang , Qi Lihua , Li Weiwei , Li Xin
Published: December 2023

The microstructure evolution and variation of impact toughness in the heat-affected zone (HAZ) of X80 pipeline steel with different Nb content under different peak temperatures in the secondary thermal cycle were studied through welding thermal simulation, the Charpy impact test, EBSD analysis, SEM observation, and TEM observation in this study. The results indicate that when the peak temperatures of the second pass were lower than Ac1, both X80 pipeline steels had high impact toughness. For secondary peak temperatures in the range of Ac1 to Ac3, both X80 pipeline steels had the worst impact toughness, mainly due to the formation of massive blocky M-A constituents in chain form on grain boundaries. When the secondary peak temperatures were higher than Ac3, both X80 pipeline steels had excellent impact toughness. Smaller grain size and higher proportions of HAGBs can effectively improve the impact toughness. Meanwhile, high Nb X80 pipeline steel had higher impact absorption energy and smaller dispersion. Adding an appropriate amount of Nb to X80 pipeline steel can ensure the impact toughness of SCCGHAZ and SCGHAZ in welded joints.
Effect of Vibrotactile Feedback on the Control of the Interaction Force of a Supernumerary Robotic Arm
Authors: Buratti Silvia , Deiana Davide , Noccaro Alessia , Pinardi Mattia , Di Pino Giovanni , Formica Domenico , Jarrassé Nathanaël
Published: December 2023

Supernumerary robotic limbs are mainly designed to augment the physical capabilities of able-bodied individuals, in a wide range of contexts from body support to surgery. When they are worn as wearable devices, they naturally provide inherent feedback due to the mechanical coupling with the human body. The user can, thus, perceive the interaction with the environment by relying on a combination of visual and inherent feedback. However, these can be inefficient in accomplishing complex tasks, particularly in the case of visual occlusion or variation in the environment stiffness. Here, we investigated whether, in a force-regulation task using a wearable supernumerary robotic arm (SRA), additional vibrotactile feedback can increase the control performance of participants compared to the inherent feedback. Additionally, to make the scenario more realistic, we introduced variations in the SRA’s kinematic posture and in the environment stiffness. Notably, our findings revealed a statistically significant improvement in user performance over all the evaluated metrics while receiving additional vibrotactile feedback. Compared to inherent feedback alone, the additional vibrotactile feedback allowed participants to exert the required force faster (p < 0.01), to maintain it for longer (p < 0.001), and with lower errors (p < 0.001). No discernible effects related to the SRA’s posture or environment stiffness were observed. These results proved the benefits of providing the user with additional vibrotactile feedback to convey the SRA’s force during interaction tasks.