bluuebunny/crossref_metadata_2025 huggingface dataset:
Aberrant hydroxymethylation in promoter CpG regions of genes related to the cell cycle and apoptosis characterizes advanced chronic myeloid leukemia disease, poor imatinib respondents and poor survival
Authors: Guru Sameer Ahmad , Sumi Mamta Pervin , Mir Rashid , Beg Mirza Masroor Ali , koner Bidhan Chandra , Saxena Alpana
Published: April 2022

Background

There is strong evidence that disease progression, drug response and overall clinical outcomes of CML disease are not only decided by BCR/ABL1 oncoprotein but depend on accumulation of additional genetic and epigenetic aberrations. DNA hydroxymethylation is implicated in the development of variety of diseases. DNA hydroxymethylation in gene promoters plays important roles in disease progression, drug response and clinical outcome of various diseases. Therefore in this study, we aimed to explore the role of aberrant hydroxymethylation in promoter regions of different tumor suppressor genes in relation to CML disease progression, response to imatinib therapy and clinical outcome.

Methods

We recruited 150 CML patients at different clinical stages of the disease. Patients were followed up for 48 months and haematological/molecular responses were analysed. Haematological response was analysed by peripheral blood smear. BCR/ABL1 specific TaqMan probe based qRT-PCR was used for assessing the molecular response of CML patients on imatinib therapy. Promoter hydroxymethylation of the genes was characterized using MS-PCR.

Results

We observed that promoter hydroxymethylation of DAPK1, RIZ1, P16INK4A, RASSF1A and p14ARFARF genes characterize advanced CML disease and poor imatinib respondents. Although, cytokine signalling (SOCS1) gene was hypermethylated in advanced stages of CML and accumulated in patients with poor imatinib response, but the differences were not statistically significant. Moreover, we found hypermethylation of p14ARF, RASSF1 and p16INK4A genes and cytokine signalling gene (SOCS1) significantly associated with poor overall survival of CML patients on imatinib therapy. The results of this study are in agreement of the role of aberrant DNA methylation of different tumor suppressor genes as potential biomarkers of CML disease progression, poor imatinib response and overall clinical outcome.

Conclusion

In this study, we report that promoter hydroxymethylation of DAPK1, RIZ1, P16INK4A, RASSF1A and p14ARFARF genes is a characteristic feature of CML disease progressions, defines poor imatinib respondents and poor overall survival of CML patients to imatinib therapy.
Studying latent change process in height growth of children in Ethiopia, India, Peru and Vietnam
Authors: Wake Senahara Korsa , Zewotir Temesgen , Muluneh Essey Kebede
Published: April 2022

Background

Anthropometric measurements of healthy children differ in different parts of the world due to the diverse ethnicity and cultural backgrounds of families. In longitudinal studies, appropriate modeling of repeated anthropometric measures can improve the understanding of patterns of change, determinants of patterns, and variations in patterns of change over time. The objective of this study was to examine the latent change in physical height of children in Ethiopia, India, Peru, and Vietnam.

Method

Longitudinal data of 6601 children aged 1 to 15 years were obtained from the Young Lives cohort study. The data were analyzed using a latent basis growth curve model.

Results

The findings of the study revealed that the rates of growth did not remain constant across the time intervals, which indicates the nonlinearity of the growth trajectory over time. For instance, children had the highest rate of growth between age 1 and 5 years, then between age 8 and 12 years, and a low rate of growth was observed between age 12 and 15 years. At the first measurement occasion (age 1 year) females were 0.826 cm (p < 0.0001) times shorter than males. The mean height at one year of age ranged from 72.13 cm in Ethiopia to 72.62 cm in India. Children in India and Vietnam had higher mean height at age one year. However, no significant difference in mean height at age one year was found between Ethiopian and Peruvian children, ($$p=0.914$$p=0.914). Peruvian and Vietnamese children grew at a faster rate, while Indian children grew at a slower rate than Ethiopian children.

Conclusion

We found substantial latent growth variations among children in four low- and middle-income countries. The latent trajectories differed by gender and country. The outcomes of the study could aid in detecting inequalities in children's height growth.
Knowledge, attitude and practice of Lebanese parents towards childhood overweight/obesity: the role of parent-physician communication
Authors: Zoghby Helene Barbe , Sfeir Elsa , Akel Marwan , Malaeb Diana , Obeid Sahar , Hallit Souheil
Published: April 2022

Background

Childhood overweight and obesity is one of the most challenging public health problems facing both developed and developing countries. Several studies carried worldwide assessed predisposing risk factors for childhood obesity, however, few addressed the Middle East region and particularly Lebanon. We aimed in our study to assess factors associated with knowledge, attitude and practice of Lebanese parents regarding childhood overweight, particularly the parent-physician communication.

Methods

This cross-sectional study conducted between September and December 2019. The questionnaire used was the standardized questionnaire of “Parent Questionnaire Curriculum” from the “We Can program” (Ways to Enhance Children’s Activity and Nutrition).

Results

A higher parent-physician communication score (Beta = 0.05) was significantly associated with higher knowledge, whereas having a higher intermediate vs low income (Beta = -0.65) was significantly associated with lower knowledge. A higher knowledge global score (Beta = 1.25), a higher parent-physician communication score (Beta = 0.18), and having a university education level compared to illiterate/primary/complementary (Beta = 2.59) were significantly associated with better attitude. A better attitude (Beta = 0.92) and a higher global knowledge score (Beta = 0.6) were significantly associated with a better practice.

Conclusion

This study found that a better parent-physician communication and higher knowledge were associated with better attitude and practice towards obesity. Given the rise in childhood obesity worldwide, identifying factors that help reduce childhood obesity, is becoming mandatory. Our results can open up to future studies addressing strategies to prevent childhood obesity in Lebanon.
Barriers and facilitators of health among older adult immigrants in the United States: an integrative review of 20 years of literature
Authors: Hawkins Maren M. , Holliday Daniel D. , Weinhardt Lance S. , Florsheim Paul , Ngui Emmanuel , AbuZahra Tala
Published: April 2022

Background

There are over seven million older adult immigrants in the United States, and that number is expected to increase. Older adult immigrants in the United States have unique factors that influence their health.

Methods

In this integrative review, we systematically review 20 years of peer-reviewed literature on the barriers (i.e. isolation, lack of English Language Proficiency, low health literacy, lack of SES resources, discrimination) and facilitators (i.e. English Language Proficiency and maintaining ones native language, social support, culturally sensitive providers, healthcare access) of health among older adult immigrants in the United States.

Results

We found differing uses of the term ‘older adult’, emphasis on the lack of homogeneity among older adult immigrants, social support and isolation as significant barriers and facilitators of older adult immigrant health, and inconsistencies in uses and definitions of acculturation. We also examined relevant theories in the literature. Based on the literature review, focusing on Acculturation Theory, Social Cognitive Theory, and Successful Aging Theory, combining these three theories with findings from the literature to create the Older Adult Immigrant Adapted Model for Health Promotion.

Conclusions

Public health strives to promote health and prevent adverse health outcomes. Our integrative review not only systematically and thoroughly explicates 20 years of literature, but the Older Adult Immigrant Adapted Model for Health Promotion, provides guidance for future research and interventions.
Modelling the potential acute and post-acute burden of COVID-19 under the Australian border re-opening plan
Authors: Angeles Mary Rose , Wanni Arachchige Dona Sithara , Nguyen Huong Dieu , Le Long Khanh-Dao , Hensher Martin
Published: April 2022

Background

Concerns have grown that post-acute sequelae of COVID-19 may affect significant numbers of survivors. However, the analyses used to guide policy-making for Australia’s national and state re-opening plans have not incorporated non-acute illness in their modelling. We, therefore, develop a model by which to estimate the potential acute and post-acute COVID-19 burden using disability-adjusted life years (DALYs) associated with the re-opening of Australian borders and the easing of other public health measures, with particular attention to longer-term, post-acute consequences and the potential impact of permanent functional impairment following COVID-19.

Methods

A model was developed based on the European Burden of Disease Network protocol guideline and consensus model to estimate the burden of COVID-19 using DALYs. Data inputs were based on publicly available sources. COVID-19 infection and different scenarios were drawn from the Doherty Institute’s modelling report to estimate the likely DALY losses under the Australian national re-opening plan. Long COVID prevalence, post-intensive care syndrome (PICS) and potential permanent functional impairment incidences were drawn from the literature. DALYs were calculated for the following health states: the symptomatic phase, Long COVID, PICS and potential permanent functional impairment (e.g., diabetes, Parkinson’s disease, dementia, anxiety disorders, ischemic stroke). Uncertainty and sensitivity analysis were performed to examine the robustness of the results.

Results

Mortality was responsible for 72-74% of the total base case COVID-19 burden. Long COVID and post-intensive care syndrome accounted for at least 19 and 3% of the total base case DALYs respectively. When included in the analysis, potential permanent impairment could contribute to 51-55% of total DALYs lost.

Conclusions

The impact of Long COVID and potential long-term post-COVID disabilities could contribute substantially to the COVID-19 burden in Australia’s post-vaccination setting. As vaccination coverage increases, the share of COVID-19 burden driven by longer-term morbidity rises relative to mortality. As Australia re-opens, better estimates of the COVID-19 burden can assist with decision-making on pandemic control measures and planning for the healthcare needs of COVID-19 survivors. Our estimates highlight the importance of valuing the morbidity of post-COVID-19 sequelae, above and beyond simple mortality and case statistics.
Relationship between Mother’s emotional intelligence, negative parenting behaviour, Preschooler’s attachment instability, and smart device overdependence
Authors: Lee Gumhee , Kim Sungjae
Published: April 2022

Background

As smart device overdependence among preschoolers could adversely affect their overall development, it is essential to understand the related factors of such overdependence. Mothers have a large influence on preschoolers; however,, the relationship between mothers’ emotional intelligence, negative parenting behaviour, preschoolers’ attachment instability, and smart device overdependence remain unclear. This study aims to develop and test a structural model to explain smart device overdependence among preschoolers.

Methods

The study collects and analyses data from January to May 2021 from 283 mothers raising children aged 3–6 years in South Korea. Questionnaires regarding mothers’ emotional intelligence and negative parenting behaviour, as well as preschoolers’ attachment instability and smart device overdependence, were used to collect data. The data were analysed by SPSS23.0 and AMOS 23.0 software and a structural equation model was constructed; p ≤ 0.05 was taken as significant.

Results

Mothers’ emotional intelligence had a direct negative relationship with mothers’ negative parenting behaviour (β = − 0.44) and an indirect negative relationship with preschoolers’ attachment instability (β = − 0.25) and preschoolers’ smart device overdependence (β = − 0.24). Mothers’ negative parenting behaviour had a direct positive relationship with preschoolers’ attachment instability (β = 0.56) and both direct and indirect positive relationships with preschoolers’ smart device overdependence (β = 0.55). Preschoolers’ attachment instability had a direct positive relationship with preschoolers’s smart device overdependence (β = 0.46).

Conclusions

Mothers’ emotional intelligence, negative parenting behaviour, and preschoolers’ attachment instability are associated with preschoolers’ vulnerability to smart device overdependence. These results are shown that more attention is needed to these variables in order to reduce preschoolers’ overdependence on smart devices. Additionally, we propose to develop and provide interventions based on these results.
Multimorbidity and its associated risk factors among older adults in India
Authors: Khan Mohd. Rashid , Malik Manzoor Ahmad , Akhtar Saddaf Naaz , Yadav Suryakant , Patel Ratna
Published: April 2022

Background

Health at older ages is a key public health challenge especially among the developing countries. Older adults are at greater risk of vulnerability due to their physical and functional health risks. With rapidly rising ageing population and increasing burden of non-communicable diseases older adults in India are at a greater risk for multimorbidities. Therefore, to understand this multimorbidity transition and its determinants we used a sample of older Indian adults to examine multimorbidity and its associated risk factors among the Indian older-adults aged 45 and above.

Methods

Using the sample of 72,250 older adults, this study employed the multiple regression analysis to study the risk factors of multimorbidity. Multimorbidity was computed based on the assumption of older-adults having one or more than one disease risks.

Results

Our results confirm the emerging diseases burden among the older adults in India. One of the significant findings of the study was the contrasting prevalence of multimorbidity among the wealthiest groups (AOR = 1.932; 95% CI = 1.824- 2.032). Similarly women were more likely to have a multimorbidity (AOR = 1.34; 95% CI = 1.282—1.401) as compared to men among the older adults in India.

Conclusion

Our results confirm an immediate need for proper policy measures and health system strengthening to ensure the better health of older adults in India.
Comparison of two teaching methods for stopping the bleed: a randomized controlled trial
Authors: Chen Shuangyi , Li Jinfei , DiNenna Michael A. , Gao Chen , Chen Shijie , Wu Song , Tang Xiaohong , He Jinshen
Published: April 2022

Background

The “Stop the Bleed” (STB) campaign has achieved remarkable results since it was launched in 2016, but there is no report on the teaching of an STB course combined with a trauma patient simulator. This study proposes the “problem-, team-, and evidence-based learning” (PTEBL) teaching method combined with Caesar (a trauma patient simulator) based on the STB course and compares its effect to that of the traditional teaching method among outstanding doctoral candidates training in haemostasis skills.

Method

Seventy-eight outstanding doctoral candidate program students in five and eight-year programs were selected as the research subjects and were randomly divided into a control group (traditional teaching method, n = 34) and an experimental group (PTEBL teaching method combined with Caesar, n = 44). Their confidence in their haemostasis skills and willingness to rescue injured victims were investigated before and after the course in both groups.

Result

Students’ self-confidence in their STB skills and the willingness to rescue improved after the class in both groups. Compared with the control group, students in the experimental group were more confident in compressing with bandages and compressing with a tourniquet after a class (compressing with bandages: control group 3.9 ± 0.8 vs. experimental group 4.3 ± 0.7, P = 0.014; compressing with a tourniquet: control group 3.9 ± 0.4 vs. experimental group 4.5 ± 0.8, P = 0.001) More students in the experimental group than the control group thought that the use of Caesar for scenario simulation could improve learning (control group 55.9% vs. experimental group 81.8%, P = 0.024), and using this mannequin led to higher teacher-student interaction (control group 85.3% vs. experimental group 97.7%, P = 0.042). The overall effectiveness of the teaching was better in the experimental group than in the control group (control group 85.3% vs. experimental group 97.7%, P = 0.042). There was a significant positive correlation between teacher-student interactions and the overall effectiveness of teaching (R = 1.000; 95% CI, 1.000–1.000; P < 0.001).

Conclusion

The PTEBL teaching method combined with Caesar can effectively improve student mastery of STB skills and overcome the shortcomings of traditional teaching methods, which has some promotional value in the training of outstanding doctoral candidates in STB skills.
Collaboration between general dental practitioners and dental hygienists: a qualitative study
Authors: Boer Joost C. L. den , van Dam Brigitte A. F. M. , van der Sanden Wil J. M. , Bruers Josef J. M.
Published: April 2022

Background

Influenced by governmental measures, collaboration in oral health care practices in the Netherlands has increased in recent decades. Previous studies on this subject have mainly concerned the composition of the staff or have been normative rather than descriptive. Based on the existing literature, four aspects were expected to be of significant influence on the collaboration on oral health care practices: goals, leadership, the allocation of tasks and responsibilities and formalization.

Methods

The aim of this study was to describe a classification of collaboration between general dental practitioners and dental hygienists within oral health care practices in the Netherlands. Eighteen semi-structured face-to-face interviews were conducted in nine oral health care practices, which differed with regard to both practice characteristics and characteristics of the principal general dental practitioner. In all practices, the principal general dental practitioner and one dental hygienist were consulted. The interviews were conducted in the practices of the respondents and were held between October 2019 and July 2020. The interviews were analyzed through theoretical thematic analysis using Microsoft Word 2010 and Atlas.ti 8. Three researchers coded and analyzed three practices, and discussed their results. Based on their mutual conclusions, one researcher analysed all practices. The final results were reviewed and approved by the other two researchers.

Results

Different factors influencing the collaboration between general dental practitioners and dental hygienist in the Netherlands were found. The most important factors seemed to be leadership style and goals for collaboration. Leadership style varied from very directive to very supportive and seemed to be connected to the allocation of responsibilities. Goals for collaboration varied from predominantly patient-related to mostly practice-related. Formalization appeared to be more present in larger practices and practices that are affiliated to a dental chain.

Conclusions

Based on leadership style and goals for collaboration, a classification was identified. This classification is recommended as a starting point for further research on directive and supportive leadership in oral health care practices.
Prediction of overall survival based upon a new ferroptosis-related gene signature in patients with clear cell renal cell carcinoma
Authors: Sun Zhuolun , Li Tengcheng , Xiao Chutian , Zou Shaozhong , Zhang Mingxiao , Zhang Qiwei , Wang Zhenqing , Zhan Hailun , Wang Hua
Published: April 2022

Background

Clear cell renal cell carcinoma (ccRCC) is the most common and lethal renal cell carcinoma (RCC) histological subtype. Ferroptosis is a newly discovered programmed cell death and serves an essential role in tumor occurrence and development. The purpose of this study is to analyze ferroptosis-related gene (FRG) expression profiles and to construct a multi-gene signature for predicting the prognosis of ccRCC patients.

Methods

RNA-sequencing data and clinicopathological data of ccRCC patients were downloaded from The Cancer Genome Atlas (TCGA). Differentially expressed FRGs between ccRCC and normal tissues were identified using ‘limma’ package in R. GO and KEGG enrichment analyses were conducted to elucidate the biological functions and pathways of differentially expressed FRGs. Consensus clustering was used to investigate the relationship between the expression of FRGs and clinical phenotypes. Univariate and the least absolute shrinkage and selection operator (LASSO) Cox regression analysis were used to screen genes related to prognosis and construct the optimal signature. Then, a nomogram was established to predict individual survival probability by combining clinical features and prognostic signature.

Results

A total of 19 differentially expressed FRGs were identified. Consensus clustering identified two clusters of ccRCC patients with distinguished prognostic. Functional analysis revealed that metabolism-related pathways were enriched, especially lipid metabolism. A 7-gene ferroptosis-related prognostic signature was constructed to stratify the TCGA training cohort into high- and low-risk groups where the prognosis was significantly worse in the high-risk group. The signature was identified as an independent prognostic indicator for ccRCC. These findings were validated in the testing cohort, the entire cohort, and the International Cancer Genome Consortium (ICGC) cohort. We further demonstrated that the signature-based risk score was highly associated with the ccRCC progression. Further stratified survival analysis showed that the high-risk group had a significantly lower overall survival (OS) rate than those in the low-risk group. Moreover, we constructed a nomogram that had a strong ability to forecast the OS of the ccRCC patients.

Conclusions

We constructed a ferroptosis-related prognostic signature, which might provide a reliable prognosis assessment tool for the clinician to guide clinical decision-making and outcomes research.
A mixed-methods systematic review of suicide prevention interventions involving multisectoral collaborations
Authors: Pearce Tania , Maple Myfanwy , Wayland Sarah , McKay Kathy , Woodward Alan , Brooks Anna , Shakeshaft Anthony
Published: April 2022

Background

Governments and third-sector organizations (TSOs) require support to reduce suicide mortality through funding of suicide prevention services and innovative research. One way is for researchers to engage individuals and services in multisectoral collaborations, to collaboratively design, develop and test suicide prevention services and programmes. However, despite widespread support, to date, it remains unclear as to the extent to which stakeholders are being included in the research process, or if they are, how these partnerships occur in practice. To address this gap, the authors conducted a systematic review with the aim of identifying evidence of multisectoral collaborations within the field of suicide prevention, the types of stakeholders involved and their level of involvement.

Methods

The authors conducted a strategic PRISMA-compliant search of five electronic databases to retrieve literature published between January 2008 and July 2021. Hand-searching of reference lists of key systematic reviews was also completed. Of the 7937 papers retrieved, 16 papers finally met the inclusion criteria. Because of data heterogeneity, no meta-analysis was performed; however, the methodological quality of the included studies was assessed.

Results

Only one paper included engagement of stakeholders across the research cycle (co-ideation, co-design, co-implementation and co-evaluation). Most stakeholders were represented by citizens or communities, with only a small number of TSOs involved in multisectoral collaborations. Stakeholder level of involvement focused on the co-design or co-evaluation stage.

Conclusion

This review revealed a lack of evidence of multisectoral collaborations being established between researchers and stakeholders in the field of suicide prevention research, even while such practice is being espoused in government policies and funding guidelines. Of the evidence that is available, there is a lack of quality studies documenting the collaborative research process. Also, results showed that the inclusion of co-researchers from communities or organizations is defined as co-creation, but further analysis revealed that collaboration was not consistent across the duration of projects. Researchers and practitioners should consider issues of power and equity in multisectoral collaborations and encourage increased engagement with TSOs, to rigorously research and evaluate suicide prevention services.
OIDA: An optimal interval detection algorithm for automatized determination of stimulation patterns for FES-Cycling in individuals with SCI
Authors: Schmoll Martin , Le Guillou Ronan , Fattal Charles , Coste Christine Azevedo
Published: April 2022

Background

FES-Cycling is an exciting recreational activity, which allows certain individuals after spinal cord injury or stroke to exercise their paralyzed muscles. The key for a successful application is to activate the right muscles at the right time.

Methods

While a stimulation pattern is usually determined empirically, we propose an approach using the torque feedback provided by a commercially available crank power-meter installed on a standard trike modified for FES-Cycling. By analysing the difference between active (with stimulation) and passive (without stimulation) torques along a full pedalling cycle, it is possible to differentiate between contributing and resisting phases for a particular muscle group. In this article we present an algorithm for the detection of optimal stimulation intervals and demonstrate its functionality, bilaterally for the quadriceps and hamstring muscles, in one subject with complete SCI on a home trainer. Stimulation patterns were automatically determined for two sensor input modalities: the crank-angle and a normalized thigh-angle (i.e. cycling phase, measured via inertial measurement units). In contrast to previous studies detecting automatic stimulation intervals on motorised ergo-cycles, our approach does not rely on a constant angular velocity provided by a motor, thus being applicable to the domain of mobile FES-Cycling.

Results

The algorithm was successfully able to identify stimulation intervals, individually for the subject’s left and right quadriceps and hamstring muscles. Smooth cycling was achieved without further adaptation, for both input signals (i.e. crank-angle and normalized thigh-angle).

Conclusion

The automatic determination of stimulation patterns, on basis of the positive net-torque generated during electrical stimulation, can help to reduce the duration of the initial fitting phase and to improve the quality of pedalling during a FES-Cycling session. In contrast to previous works, the presented algorithm does not rely on a constant angular velocity and thus can be effectively implemented into mobile FES-Cycling systems. As each muscle or muscle group is assessed individually, our algorithm can be used to evaluate the efficiency of novel electrode configurations and thus could promote increased performances during FES-Cycling.
Endocrine cell micronests in an ovarian mucinous borderline tumor: a potential diagnostic pitfall for microinvasion
Authors: Collins Katrina , Segura Sheila , Hwang Michael
Published: April 2022

The occurrence of endocrine cell micronests in ovarian tumors is rarely reported. To our knowledge, there are only three prior cases reported to date: one occurring in an ovarian mucinous cystadenoma, one in an ovarian mucinous cystadenofibroma, and another in an ovarian mucinous carcinoma with a predominant borderline component. This is a 27-year-old woman that presented with a one-month history of abdominal pain and fullness. Imaging studies revealed a large multiloculated cystic and solid mass measuring 23 cm occupying the majority of the pelvis and abdomen concerning for a primary ovarian malignancy. The patient underwent a right salpingo-oophorectomy with appendectomy. Histologic sections from the ovary showed a multiloculated, cystic and focally solid mass lined by gastrointestinal-type mucinous epithelium with variable degrees of proliferation accounting for greater than 10% of the tumor. In addition to the mucinous epithelial component, there were several foci of bland, monotonous epithelioid cells arranged in solid nests with focal tubular/acinar formation within the fibrous septa and mucinous epithelium. Immunohistochemical studies showed that these cells were positive for cytokeratin, EMA, and synaptophysin, while negative for inhibin. The Ki-67 proliferation index was low (<1%). The presence of endocrine cell nests associated with an ovarian mucinous neoplasm is a rare phenomenon. Whether this represents preservation of endocrine cells in the context of epithelial degeneration or an independent neoplastic component is unclear. Progression related to this endocrine cell proliferation is unlikely and the recognition of this phenomenon holds more diagnostic value than prognostic significance, as it could be confused with microinvasion or sex cord stromal elements.
Serum IL-17 levels are higher in critically ill patients with AKI and associated with worse outcomes
Authors: Collett Jason A. , Ortiz-Soriano Victor , Li Xilong , Flannery Alexander H. , Toto Robert D. , Moe Orson W. , Basile David P. , Neyra Javier A.
Published: April 2022

Background

Interleukin-17 (IL-17) antagonism in rats reduces the severity and progression of AKI. IL-17-producing circulating T helper-17 (TH17) cells is increased in critically ill patients with AKI indicating that this pathway is also activated in humans. We aim to compare serum IL-17A levels in critically ill patients with versus without AKI and to examine their relationship with mortality and major adverse kidney events (MAKE).

Methods

Multicenter, prospective study of ICU patients with AKI stage 2 or 3 and without AKI. Samples were collected at 24–48 h after AKI diagnosis or ICU admission (in those without AKI) [timepoint 1, T1] and 5–7 days later [timepoint 2, T2]. MAKE was defined as the composite of death, dependence on kidney replacement therapy or a reduction in eGFR of ≥ 30% from baseline up to 90 days following hospital discharge.

Results

A total of 299 patients were evaluated. Patients in the highest IL-17A tertile (versus lower tertiles) at T1 had higher acuity of illness and comorbidity scores. Patients with AKI had higher levels of IL-17A than those without AKI: T1 1918.6 fg/ml (692.0–5860.9) versus 623.1 fg/ml (331.7–1503.4), p < 0.001; T2 2167.7 fg/ml (839.9–4618.9) versus 1193.5 fg/ml (523.8–2198.7), p = 0.006. Every onefold higher serum IL-17A at T1 was independently associated with increased risk of hospital mortality (aOR 1.35, 95% CI: 1.06–1.73) and MAKE (aOR 1.26, 95% CI: 1.02–1.55). The highest tertile of IL-17A (vs. the lowest tertile) was also independently associated with higher risk of MAKE (aOR 3.03, 95% CI: 1.34–6.87). There was no effect modification of these associations by AKI status. IL-17A levels remained significantly elevated at T2 in patients that died or developed MAKE.

Conclusions

Serum IL-17A levels measured by the time of AKI diagnosis or ICU admission were differentially elevated in critically ill patients with AKI when compared to those without AKI and were independently associated with hospital mortality and MAKE.
Increasing participation in habitual intellectual activities on modulating functional connectivity of default mode network among older adults at risk of dementia: study protocol of a randomized controlled trial
Authors: Yan Rachel W. K. , Kwok Charlotte P. C. , Kwok Jessie O. T. , Lee Kaspar K. W. , Lu Hanna , Chu Winnie C. W. , Kwok Timothy C. Y. , Lam Linda C. W. , Lee Allen T. C.
Published: April 2022

Background

Active participation in intellectual leisure activities such as calligraphy helps prevent cognitive decline and dementia, but the underlying mechanisms are not fully understood. With disrupted functional connectivity (FC) of default mode network (DMN) associated with cognitive decline, we speculate that intellectual activities might optimize cognitive function through modulating FC of DMN. This two-arm single-blind randomized controlled trial aims to identify the effects of increasing practice of calligraphy on cognitive function and FC of DMN in people with subjective cognitive decline (SCD).

Methods

One hundred twelve community-living Chinese aged 55 to 75 years old with SCD but without mild cognitive impairment or dementia and with prior practice of calligraphy as defined by 1 h of calligraphy per week will be recruited through elderly social centres in Hong Kong and randomized into either control or intervention group. The control group will continue with their usual practice of calligraphy, whereas the intervention group will double their practice of calligraphy. Measurement of cognitive outcomes and neuroimaging on resting-state FC will be performed at baseline and in 6 months. Repeated measures analysis of variance will be used to assess cognitive and FC changes, with time being the within-group factor, control/intervention as the between-group measure, and important covariates (age, sex, educational and occupational attainment, health, and other lifestyle factors) controlled for.

Discussion

This study will shed light on the underlying neurocognitive mechanisms of how intellectual activities promotes cognitive maintenance. Our anticipated findings will provide evidence that reversing or slowing FC disruption by actively participating in intellectual activities is still possible for the at-risk individuals.

Trial registration

Chinese Clinical Trial Registry ChiCTR1900024433. Registered on 11 July 2019.
Spruce (Picea abies L.) planting leads post-clearcut bird assemblages to a novel successional pathway—a comparative study in hemiboreal mixed forests
Authors: Pass Eliisa , Kont Raido , Lõhmus Asko
Published: April 2022

Key message

Bird assemblages in planted spruce (Picea abiesL.) forests were compositionally distinct from those in naturally regenerated mixed stands. Despite rapid stand development on productive soils, even-aged silviculture cannot host most old-forest species. However, their habitat quality could be supported in landscape mosaics with retention forestry and set-asides.

Context

The most productive forest lands have naturally the richest bird assemblages but tend to be also most intensively managed. Sustainable solutions to this conflict are unclear.

Aim

To assess bird assemblages and their successional dynamics in planted Norway spruce (Picea abiesL.) stands compared to naturally developing stands.

Methods

We mapped breeding bird assemblages in forty 5-ha plots on highly productive soils in Estonia. The plots included sets of naturally regenerated and planted stands, and (as successional endpoints) clear-cuts and old stands.

Results

Planted stands had fewer bird species and pairs than naturally regenerated stands; the latter having a species composition resembling late-successional deciduous-dominated stands. Importantly, the species composition in mature spruce plantations converged toward the composition observed in late-successional conifer-dominated stands. Downed dead wood, stand age, deciduous trees, and stock density were the most significant stand characteristics shaping bird assemblages.

Conclusion

The habitat value of established spruce plantations can be primarily improved by allowing for some deciduous trees and gaps with deciduous undergrowth. At the landscape scale, the bird diversity of even-aged systems would be enhanced by multi-scale applications of retention forestry—from retention trees to old-growth set-asides.
Long-term Weight Change and its Temporal Relation to Later-life Dementia in the Health and Retirement Study
Authors: Shen Jie , Chen Hui , Zhou Tianjing , Zhang Simei , Huang Liyan , Lv Xiaozhen , Ma Yuan , Zheng Yan , Yuan Changzheng
Published: April 2022

Context

Weight loss among middle-aged and older adults has been associated with a higher risk of subsequent dementia. However, most studies have limited follow-up durations or suboptimal control for the potential influence of physical frailty (PF).

Objective

Our study aimed to investigate the long-term and temporal relations of weight change to risk of dementia among middle-aged and older adults in the United States.

Methods

A total of 5985 participants aged 65 years and older were included from the Health and Retirement Study. History of long-term weight change was calculated using 9 repeated body mass index measurements during 1992-2008. We then followed participants’ dementia status from 2008 to 2018. Multivariable Cox proportional hazard models were used.

Results

During the study follow-up period (mean = 7.54 years), a total of 682 (11.40%) dementia cases were documented. After adjustment for basic demographic and lifestyle factors, participants with weight loss (median: −0.23 kg/m2 per year) were at a significantly higher risk of dementia (HR = 1.60; 95% CI, 1.33, 1.92), compared with the stable weight group (median: 0.11 kg/m2 per year). This association was attenuated but remained strong and significant after further adjustment for PF (HR = 1.57; 95% CI, 1.30, 1.89). Significant association was observed for weight loss assessed approximately 14 to 18 years preceding dementia diagnosis (HR = 1.30; 95% CI, 1.07, 1.58), and was consistent closer to diagnosis.

Conclusion

Both recent and remote weight loss were associated with a higher risk of later-life dementia among middle-aged and older adults independent of PF status.
Avian auditory hair cell regeneration is accompanied by JAK/STAT-dependent expression of immune-related genes in supporting cells
Authors: Janesick Amanda S. , Scheibinger Mirko , Benkafadar Nesrine , Kirti Sakin , Heller Stefan
Published: April 2022

The avian hearing organ is the basilar papilla that, in sharp contrast to the mammalian cochlea, can regenerate sensory hair cells and thereby recover from deafness within weeks. The mechanisms that trigger, sustain and terminate the regenerative response in vivo are largely unknown. Here, we profile the changes in gene expression in the chicken basilar papilla after aminoglycoside antibiotic-induced hair cell loss using RNA-sequencing. We identified changes in gene expression of a group of immune-related genes and confirmed with single-cell RNA-sequencing that these changes occur in supporting cells. In situ hybridization was used to further validate these findings. We determined that the JAK/STAT signaling pathway is essential for upregulation of the damage-response genes in supporting cells during the second day after induction of hair cell loss. Four days after ototoxic damage, we identified newly regenerated, nascent auditory hair cells that express genes linked to termination of the JAK/STAT signaling response. The robust, transient expression of immune-related genes in supporting cells suggests a potential functional involvement of JAK/STAT signaling in sensory hair cell regeneration.
The immune environment of the mammary gland fluctuates during post-lactational regression and correlates with tumour growth rate
Authors: Hitchcock Jessica , Hughes Katherine , Pensa Sara , Lloyd-Lewis Bethan , Watson Christine J.
Published: April 2022

Post-lactational mammary gland regression encompasses extensive programmed cell death and removal of milk-producing epithelial cells, breakdown of extracellular matrix components and redifferentiation of stromal adipocytes. This highly regulated involution process is associated with a transient increased risk of breast cancer in women. Using a syngeneic tumour model, we show that tumour growth is significantly altered depending on the stage of involution at which tumour cells are implanted. Tumour cells injected at day 3 involution grew faster than those in nulliparous mice, whereas tumours initiated at day 6 involution grew significantly slower. These differences in tumour progression correlate with distinct changes in innate immune cells, in particular among F4/80-expressing macrophages and among TCRδ+ unconventional T cells. Breast cancer post-pregnancy risk is exacerbated in older first-time mothers and, in our model, initial tumour growth is moderately faster in aged mice compared with young mice. Our results have implications for breast cancer risk and the use of anti-inflammatory therapeutics for postpartum breast cancers.
Natural killer cells act as an extrinsic barrier for in vivo reprogramming
Authors: Melendez Elena , Chondronasiou Dafni , Mosteiro Lluc , Martínez de Villarreal Jaime , Fernández-Alfara Marcos , Lynch Cian J. , Grimm Dirk , Real Francisco X. , Alcamí José , Climent Núria , Pietrocola Federico , Serrano Manuel
Published: April 2022

The ectopic expression of the transcription factors OCT4, SOX2, KLF4 and MYC (OSKM) enables reprogramming of differentiated cells into pluripotent embryonic stem cells. Methods based on partial and reversible in vivo reprogramming are a promising strategy for tissue regeneration and rejuvenation. However, little is known about the barriers that impair reprogramming in an in vivo context. We report that natural killer (NK) cells significantly limit reprogramming, both in vitro and in vivo. Cells and tissues in the intermediate states of reprogramming upregulate the expression of NK-activating ligands, such as MULT1 and ICAM1. NK cells recognize and kill partially reprogrammed cells in a degranulation-dependent manner. Importantly, in vivo partial reprogramming is strongly reduced by adoptive transfer of NK cells, whereas it is significantly increased by their depletion. Notably, in the absence of NK cells, the pancreatic organoids derived from OSKM-expressing mice are remarkably large, suggesting that ablating NK surveillance favours the acquisition of progenitor-like properties. We conclude that NK cells pose an important barrier for in vivo reprogramming, and speculate that this concept may apply to other contexts of transient cellular plasticity.
Effect of prenatal glucocorticoids and thyroid hormones on developmental plasticity of mitochondrial aerobic metabolism, growth and survival: an experimental test in wild great tits
Authors: Cossin-Sevrin Nina , Hsu Bin-Yan , Marciau Coline , Viblanc Vincent A. , Ruuskanen Suvi , Stier Antoine
Published: April 2022

Developmental plasticity is partly mediated by transgenerational effects, including those mediated by the maternal endocrine system. Glucocorticoid and thyroid hormones may play central roles in developmental programming through their action on metabolism and growth. However, the mechanisms by which they affect growth and development remain understudied. One hypothesis is that maternal hormones directly affect the production and availability of energy-carrying molecules (e.g. ATP) by their action on mitochondrial function. To test this hypothesis, we experimentally increased glucocorticoid and thyroid hormones in wild great tit eggs (Parus major) to investigate their impact on offspring mitochondrial aerobic metabolism (measured in blood cells), and subsequent growth and survival. We show that prenatal glucocorticoid supplementation affected offspring cellular aerobic metabolism by decreasing mitochondrial density, maximal mitochondrial respiration and oxidative phosphorylation, while increasing the proportion of the maximum capacity being used under endogenous conditions. Prenatal glucocorticoid supplementation only had mild effects on offspring body mass, size and condition during the rearing period, but led to a sex-specific (females only) decrease in body mass a few months after fledging. Contrary to our expectations, thyroid hormone supplementation did not affect offspring growth or mitochondrial metabolism. Recapture probability as juveniles or adults was not significantly affected by prenatal hormonal treatment. Our results demonstrate that prenatal glucocorticoids can affect post-natal mitochondrial density and aerobic metabolism. The weak effects on growth and apparent survival suggest that nestlings were mostly able to compensate for the transient decrease in mitochondrial aerobic metabolism induced by prenatal glucocorticoids.
Additions to the knowledge of the diversity of darkling beetles (Coleoptera: Tenebrionidae) from Turkey with new records and taxonomic notes
Authors: NABOZHENKO MAXIM , DOĞAN DILEK , YILDIRIM EROL
Published: April 2022

This study is based on the material of darkling beetles (Tenebrionidae) collected from different localities of Turkey between 1966 and 2020 and deposited in Biodiversity Science Museum, Atatürk University (Erzurum, Turkey). In total, 80 species and subspecies of the family Tenebrionidae were listed. The following five species are recorded for Turkey for the first time: Cyphostethe (Cyphostethoides) semenovi Bogatchev, 1947, Zophosis (Septentriophosis) rugosa Faldermann, 1837, Penthicus (Discotus) dilectans (Faldermann, 1836), Penthicus (s. str.) rufescens rufescens (Mulsant et Rey, 1859) and Scleropatroides hirtulus (Baudi di Selve, 1876). In addition, new localities have been indicated for many species and subspecies, which have already been reported from Turkey. The new synonymy is proposed: Blaps tibialis Reiche & Saulcy, 1857 = Blaps ecaudata Seidlitz, 1893, syn. nov. The invasive population of the Mediterranean species Euboeus mimonti Boieldieu, 1865 was found in Erzurum but the species has not naturalised in Eastern Anatolia.
Cross-fringe piston sensor for segmented optics
Authors: Sato Seichi , Mizutani Tadahito
Published: April 2022

This paper presents a simple slit-mask-based sensing scheme that can be applied to a robust piston error sensor for segmented optical systems, even in space. Utilizing crossed fringes generated from multiple double-slit pairs, the sensor simultaneously detects multiple points in a segmented optical system in one shot. The experiments tested six pistons of a single-ring segmented mirror. The results showed measurement stability better than 15 nm in the range of 0–5 µm and absolute accuracy of

λ

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20

. The theoretical perspective of applicability for larger piston measurement and N-ring segmented systems is also discussed, with simulation results for a two-ring system presented.
Spreading evolution of a linear phase-locked hollow beam array in atmospheric turbulence
Authors: Zhao Jie , Dong Rui , Li Yaping , Zou Jiayi , Qiao Chunhong , Lu Lu
Published: April 2022

Based on the generalized Huygens–Fresnel principle, propagation characteristics of a linear phase-locked hollow beam (PLHB) array propagating through atmospheric turbulence are investigated. Analytical expressions of the average intensity and mean-squared beam width of the PLHB array are derived. It is shown that propagation properties of a PLHB array are related to the initial beam parameters (i.e., number of beamlets, size of beam waist, range of wavelength) of the PLHB array and the strength of turbulence. These findings may provide potential applications in free-space optical communications, lidar, remote sensing, and related fields.