bluuebunny/crossref_metadata_2025 huggingface dataset:
The Use of Modified Bipolar Cutting & Coagulation System versus Harmonic FOCUS in Total Thyroidectomy
Authors: Wei Gang , Li Rui , Wu Gaosong
Published: January 2024

Aims and Objectives: We aimed to determine whether the modified bipolar
cutting & coagulation system resulted in shorter operative time and
less intraoperative blood loss and less postoperative complications
compared to harmonic FOCUS for total thyroidectomy. Method: From
September 2016 to December 2019, a total of 305 inpatients underwent
total thyroidectomy in our center were randomly divided into trial
group(n=155) and control group(n=150) and the surgical results were
analysed prospectively. Results: The operative time, intraoperative
blood loss, specimen weight, the classification of cervical lymph node
dissection and the mean number of dissected lymph nodes indicated no
statistical difference between two groups(P＞0.05). The rate of
intraoperative parathyroid autotransplantation was 32.7%(49/150) in the
control group, significantly higher than 16.1%(25/155) in the trial
group (P=.001). The levels of serum PTH in the trial group at day 1,3
and 5 were significantly lower than those in the trail group(P＜0.04).
The rate of transient RLN injury was 8.0%(12/150) in the control group,
significantly higher than 2.6%(4/155) in the trail group(P=.041). The
rate of postoperative transient hypocalcemia were 33.3% (50/150) in the
control group, more than 14.8%(23/155) in the trail group
statistically((P=.000). Conclusions: The modified bipolar cutting &
coagulation system means safe, less damage and less complications and
deserves popularizing for thyroid surgery.
Parasitic plants indirectly regulate decomposition of soil organic matter
Authors: Yuan Yongge , Lin Xinru , Chen Gelv , Kleunen Mark van , Li Junmin
Published: January 2024

Parasitic plants have been shown to affect soil-organic-matter (SOM)
decomposition, but the mechanism is unknown. As arbuscular mycorrhizal
fungi (AMF) can affect decomposition and compete with parasitic plants
for carbon, we hypothesized that parasitic plants can indirectly
regulate decomposition by suppressing AMF. We conducted two experiments
in which Bidens pilosa was inoculated with AMF or not, and parasitized
with Cuscuta australis or not. In hyphae-in-growth bags, we provided SOM
in one experiment as 13C-/15N-labelled maize leaves and in the other as
phytate P. In the presence of AMF, Parasitization increased the 13C but
decreased the organic P remaining in the bags. In the absence of the
parasite, AMF decreased the 13C and increased the organic P remaining in
the bags. Our results demonstrate that parasitic plants can indirectly
regulate the decomposition of organic materials by suppressing the
effect of the extraradical AMF hyphae on decomposition.
A sweet cherry glutathione S-transferase gene, PavGST1, plays a central role in fruit skin coloration
Authors: Qi Xiliang , Dong Yuanxin , Liu Congli , Song Lulu , Chen Lei , Li Ming
Published: January 2024

Sweet cherry, an economically important horticultural crop, has strong
antioxidant activity. The fruits contain compounds potentially
beneficial to human health─particularly anthocyanins, which are
synthesized in cytosol and predominantly accumulated in vacuoles.
Although anthocyanin levels differ among dark-red, blush, and yellow
sweet cherry cultivars, the regulatory mechanism of anthocyanin
transport and accumulation is not well understood in this species. In
this study, we identified 53 glutathione S-transferase genes (PavGSTs)
from sweet cherry and found that PavGST1 expression was well correlated
with anthocyanin accumulation in cultivars with different fruit skin
colors. TRV-mediated virus-induced silencing of PavGST1 decreased
anthocyanin accumulation in sweet cherry fruits and downregulated the
expressions of anthocyanin biosynthetic and regulatory genes. In
addition, transient overexpression of PavGST1 promoted anthocyanin
accumulation. Furthermore, yeast one-hybrid and dual-luciferase assays
revealed that PavMYB10.1 and PavMYB75 directly bind to different MYB
binding sites of the PavGST1 promoter (MBS-1 and MBS-3) to activate
PavGST1 transcription. According to our results, PavGST1 plays a central
role in sweet cherry fruit anthocyanin accumulation. Our findings
provide novel insights into the coordinative regulatory mechanisms of
PavGST1 and PavMYBs in anthocyanin accumulation in sweet cherry.
Evergreen broadleaf greenness and its relationship with leaf flushing, aging, and water fluxes
Authors: Luo Yunpeng , Pacheco-Labrador Javier , Richardson Andrew , Seyednasrollah Bijan , Perez-Priego Oscar , Cascon Rosario Gonzalez , Martí M. Pilar , Moreno Gerardo , Nair Richard , Wutzler Thomas , Bucher Solveig Franziska , Carrara Arnaud , Cremonese Edoardo , El-Madany Tarek , Filippa Gianluca , Galvagno Marta , Hammer Tiana , Ma Xuanlong , Martini David , Zhang Qian , Reichstein Markus , Menzel Annette , Römermann Christine , Migliavacca Mirco
Published: January 2024

Remote sensing capabilities to monitor evergreen broadleaved vegetation
are limited by the low temporal variability in the greenness signal.
With canopy greenness computed from digital repeat photography
(PhenoCam), we investigated how canopy greenness related to seasonal
changes in leaf age and traits as well as variation of trees’ water
fluxes (characterized by sap flow and canopy conductance). The results
showed sprouting leaves, with significantly different leaf traits
compared to mature and old leaves, are mainly responsible for the rapid
increase in canopy green chromatic coordinate (GCC) in spring. Thus, the
temporal dynamics of canopy GCC can be explained by leaf spectral
properties and leaf age. Air temperature and vapor pressure deficit
(VPD) explained most of sap flow and canopy conductance variance,
respectively. Besides, GCC is an important explanatory variable for
variation of canopy conductance may because GCC can represent the leaf
ontogeny information. We conclude that PhenoCam GCC can be used to
identify the new leaf flushing for evergreen broadleaved trees, which
carries important information about leaf ontogeny and traits thus can
better estimate of water fluxes such as canopy conductance.
Factors and Mechanism Affecting the Secretion of Recombinant Protein in CHO Cells
Authors: Yang Yong Xiao , Li Qin , Li Wei-Dong , Wang Tian-yun , Feng Hui-Gen
Published: January 2024

The market demand for biopharmaceuticals, the leading drugs in the
pharmaceutical industry, has promoted the development of various protein
expression hosts and bioprocessing technologies. While advances in the
field of recombinant protein production have changed the previous
situation, resulting in increased production and reduced costs,
industrialized production helped in the treatment of a variety of
diseases. Since mammalian cells have the unique advantages of being able
to direct the correct folding of proteins and provide post-translational
processing such as complex glycosylation the recombinant protein drugs
produced by them currently account for approximately 80% of the
approved marketed protein drugs, while Chinese hamster ovary (CHO) cells
are currently the preferred host cells for the production of recombinant
protein drugs. Obtaining recombinant protein drugs involves the
synthesis, processing, transport, and secretion of proteins, and the
secretion of proteins greatly limits the manufacturing of recombinant
protein drugs. Here, we review the current research status of CHO cells
as an important engineering tool for recombinant protein drug expression
systems, focus on the recombinant protein secretion process of CHO cells
and its influencing factors, and discuss the optimization strategy for
recombinant protein secretion and expression in CHO cells.
Flufenamic acid improves survival and neurologic outcome after successful cardiopulmonary resuscitation in mice
Authors: Chen Jiancong , Chang Yuan , Zhu Juan , Peng Yuqin , Li Zheqi , Zhang Kunxue , Zhang Yuzhen , Lin Chuman , Lin Zhenzhou , Pan Suyue , Huang Kaibin
Published: January 2024

Background Brain injury is the main cause of high mortality and
disability after successful cardiopulmonary resuscitation (CPR) from
sudden cardiac arrest (CA). Transient receptor potential M4 (TRPM4)
channel is a novel target for ameliorating blood-brain barrier (BBB)
disruption and neuroinflammation. Herein, we tested whether flufenamic
acid (FFA), which is reported to block TRPM4 with high potency, confers
neuroprotection against brain injury secondary to CA/CPR and whether the
action is exerted by blocking the TRPM4 channel. Methods Wild-type (WT)
and Trpm4 knockout (Trpm4−/−) mice subjected to 10-min CA/CPR were
randomized to receive FFA or vehicle once daily. Post-CA/CPR brain
injuries including neurological deficits, survival rate, histological
damages, edema formation, BBB destabilization and neuroinflammation were
assessed. Results In WT mice subjected to CA/CPR, FFA was effective in
improving survival and neurologic outcome, reducing neuropathological
injuries, attenuating brain edema, lessening leakage of IgG, restoring
tight junction protein expression as well as promoting
microglia/macrophages from the pro-inflammatory subtype towards the
anti-inflammatory one. In comparison to WT mice, Trpm4−/− mice exhibited
less neurologic deficiency, lighter histological impairment, more
integrity of BBB and more anti-inflammatory microglia/macrophages
polarization. As expected, FFA did not provide a benefit of
superposition compared with vehicle in the Trpm4−/− mice after CA/CPR.
Conclusions FFA mitigates BBB breach and modifies the functional status
of microglia/macrophages, thereby improving survival and neurological
deficits following CA/CPR. The neuroprotective effects are at least
partially through interfering with the TRPM4 channel. These results
provide significant clinical potentials to improve the prognosis for CA
victims with successful resuscitation.
Two-component EvgS protein subdues both antimicrobial resistance and virulence in Shigella flexneri 2a str. 301
Authors: Naha Aniket , Ramaiah Sudha
Published: January 2024

Shigella flexneri 2a is one of the leading bacterial agents of
diarrhoeal mortality in humans. Recently, multi-drug resistance strains
have posed severe challenges. The present study was conducted to
identify potential drug-target using gene interaction network (GIN)
approach in order to understand the molecular mechanisms of both
antimicrobial resistance (AMR) and virulence posed by the bacteria.
Differential gene expression (DGE) and structural analysis were
incorporated to validate our findings. Functional enrichments and
topological matrices revealed evgS, ybjZ, tolC, gyrA, parC and
their direct interactors to be associated with diverse AMR mechanisms.
Since the druggability of TolC, GyrA, and ParC has already been
exploited, the current study explored EvgS as a potential alternate
drug-target candidate due to its highest prevalence in both GINs
interconnecting several genes of two component system (TCS). DGE
patterns in ΔPhoPQ (deleted regulatory PhoP and sensor PhoQ) led to the
upregulation of TCS comprising EvgSA thereby validating EvgS as a
promising therapeutic biomarker. Druggability and structural stability
of EvgS was assessed through thermal shifts, backbone stability and
coarse dynamics refinement. Structure-function relationship was
established revealing the C-terminal extracellular domain as the drug
binding site which was further validated through molecular dynamics
simulation. Our findings would be pivotal for experimental biologists in
designing effective lead molecules against EvgS for establishing
successful therapeutic remedies against shigellosis.
Metabolic signatures in follicular fluid from human ovarian follicles during growth progression reveals that LPC as a predictor of follicular development and ovarian sensitivity
Authors: Yang Ji hong , Li Yangbai , Li Suying , Zhang Yan , Feng Ruizhi , Huang Rui , Chen Min jian , Qian Yun
Published: January 2024

Objective To investigate the metabolic characteristics of follicular
fluid (FF) from human ovarian follicles of different developmental
stages, and whether there are metabolic predictors of follicular
development (FD) with clinical implications. Design A population-based
study. Setting A two-stage study from a reproductive medicine center in
Nanjing, China between 2019 and 2020. Population A total of 226
infertile women, with tubal complications or unexplained sterility,
experiencing their first in vitro fertilization cycle. Methods and main
outcome measures The FF from both large follicles (LFs) and
matched-small follicles (SFs) was analyzed with the wide range of
targeted metabolome technique. The metabolic signatures were described
by multi-omics integration technology including our metabolomic data and
published transcriptomic data. The potential biomarkers of FD were next
verified using enzyme-linked immunoassay with FF and blood serum from an
independent 200 participants. Results Overall, 116 differential
metabolites were found in 26 LFs and 26 SFs. The combination of pathway
analysis and network analysis revealed the importance of
lysophosphatidylcholine (LPC) metabolism in FF during FD. Moreover,
differential metabolites and mRNAs were connected into a network. Upon
linking metabolites with clinical information, followed by an
independent population verification, we found that LPC was able to
predict FD and ovarian sensitivity parameters both in FF and blood
serum. Conclusions We described the FF metabolic signatures from ovarian
follicles of different developmental stages, and LPC can be used as a
biomarker of FD and ovarian sensitivity, offering potential detection
and therapeutic targets for enhancing follicle and oocyte health in
humans.
eDNA metabarcoding as a promising conservation tool to monitor fish diversity in Beijing water systems compared with ground cages
Authors: Shen Mei , Xiao Nengwen , Zhao Ziyi , Guo Ningning , Luo Zunlan , Sun Guang , Li Junsheng
Published: January 2024

Fish diversity, an important indicator of the health of aquatic
ecosystems, is declining sharply due to water pollution, overfishing,
climate change, and species invasion. Effective surveying and monitoring
are prerequisites for protecting fish diversity. In this study, eDNA
(environmental DNA）metabarcoding and ground cages were used to survey
the fish diversity of the Chaobai and Beiyun Rivers in Beijing. Based on
the two methods, the results showed that 40 species, belonging to 35
genera, 18 families, and six orders, were identified. The richness of
fish identified by eDNA metabarcoding was higher than that captured by
ground cages in both rivers. All fish captured by the ground cage method
were recognized by eDNA metabarcoding, except Squalidus wolterstorffi
and Saurogobio dabryi, which were captured only in ground cages. The
correlation of relative abundance between the two methods was affected
by the properties of the rivers, such as the flow rate. The relative
abundance detected by eDNA metabarcoding was positively correlated with
that of the ground cages in the Beiyun River, while no correlation was
observed for the Chaobai River. Our results also suggest that the
Chaobai River has higher fish diversity and a different population
structure than the Beiyun River. The primary fish species of the Beiyun
River are mainly pollution resistant. This results show that eDNA
metabarcoding as a new survey tool has great application prospects. It
provides certain theoretical data and methodological references for the
protection and management of river fish diversity.
Perceptions and Attitudes Towards Medical Marijuana in Adolescents and Young Adult Cancer Patients and their Caregivers
Authors: Asare Julie , Puthenpura Vidya , Ross Wilhelmenia , Marks Asher
Published: January 2024

Background: Oncology patients frequently have poor symptom control,
particularly for pain and nausea. The use of medical marijuana (MMJ) has
become more accepted, despite overall scant evidence of efficacy. As the
majority of states have legalized MMJ, it is important for oncology
providers to be able to counsel families regarding the role of MMJ. We
examined the perceptions of adolescent and young adult (AYA) oncology
patients and caregivers regarding MMJ to help providers frame their
discussions. Methods: Questionnaires assessing history of recreational
drug use, opinions regarding the role of MMJ in symptom management,
perceived benefits, and side effects of MMJ were administered to AYA
oncology patients and caregivers at three time points during treatment.
Results: Twenty-four patients and 26 caregivers were enrolled. The
majority of the patients and caregivers felt that MMJ should be
discussed. Prior to treatment, caregivers perceived MMJ to have greater
benefits than patients did. Knowing someone with substance abuse made
patients and caregivers more likely to believe that MMJ would increase
the risk of substance abuse and to perceive worse side effects from MMJ.
Although not statistically significant, caregivers who had previously
used marijuana tended to be more willing for their children to try MMJ
for symptom relief than caregivers with no history of use. Conclusions:
Understanding families’ prior experiences with substance abuse is
important when counseling them regarding MMJ during the patient’s
treatment. In general, families reported that MMJ should be discussed,
but this can be influenced by the families’ prior experience.
Nurse Job Change Due to Work Stress
Authors: Baffoe Seth
Published: January 2024

Abstract Rationale, Aims and Objectives: To examine the relationship
between nurse employment setting and nurse job change as related to work
stress. Method: A retrospective quantitative analysis. The secondary
data was from the 2008 National Sample Survey of Registered Nurses
(NSSRN). The outcome measure was job change related to nursing work
stress (yes vs. no). The independent variable was nurse work settings,
including covariates. Results: Nurses working in medical/physician
practice, an insurance company or other private
claims/benefits/utilization, and other settings, including nurses who
were neither satisfied nor dissatisfied, moderately dissatisfied, and
extremely dissatisfied, had higher odds for job change due to stress
when compared to inpatient unit community hospital /medical center,
non-federal and moderately satisfied the reference category,
respectively. Nurses who were unsure and had no plans to remain in the
profession had higher odds for job change due to stress than a nurse who
said yes to staying in the profession, the reference category. However,
nurses in the 65 to 69 age cohort had substantially lower ([OR] =
.482, p = .003) odds for not changing jobs due to stress compared to the
age group between 50 to 54, the reference category. Conclusion: Among
nurses, workplace job dissatisfaction translates to job change related
to nursing work stress, while nurses aged 65 to 69 stayed at the job
despite work stress. Given the current coronavirus (COVID-19)
high-stress pandemic environment, healthcare organizations must do more
to mitigate work-related job stress to prevent attrition and job change.
KEYWORDS nurses, job change, work stress, and workplace
Distribution characteristics and influencing factors of soil organic carbon in alpine desert ecosystem of Qinghai Province
Authors: Du Yangong , Ke Xun , Guo Xiaowei , Chen Kelong , cao guangmin
Published: January 2024

Alkaline deserts soils acted as important soil pools in arid and
semiarid regions. Contents of soil organic carbon (SOC) and its driven
factors remained still not clear in alpine deserts on the Tibetan
Plateau. In this study, we analyzed 223 soil organic carbon contents and
total nitrogen and pH values, and its space distribution pattern under
0-30 cm. It was indicated that average and median SOC were approximate
4.86 and 3.80 g/kg with variation coefficient of 81.14%. SOC contents
ranged from 0.54 to 24.34 g/kg. Soils organic carbon contents were
divided into four groups. The largest group was around 3.32 g/kg (145
sites) when air temperature and altitude were higher than 1.49 oC and
2793 m. Alpine desert SOC were mainly controlled by total nitrogen (TN)
and pH and precipitation with R2 of 0.87 (P<0.001).
Furthermore, soil pH was positively affected by air temperature not
precipitation (P<0.05). Models may predict SOC through
precipitation, air temperature and altitude (R2= 0.40,
P<0.001). In addition, increasing regional precipitation
perhaps decreased desert soils organic carbon storage in future climate
scenarios.
Optimal Order Iterative Methods
Authors: li xiang , Wang xue , Wei jia
Published: January 2024

Based on Newton’s method and interpolation method, we establish two
iterative methods for solving the nonlinear equations by using n+1
evaluations of the function(or the first derivative) per iteration.
Analysis of convergence shows that our methods arrive at the optimal
order of convergence 2^n, where the n is any a nature number. This
work also proves the conjecture of Kung-Traub (J.ACM643-651,1974) for
constructing multipoint optimal iterations.
Hypersensitivity reactions to Liposomal Amphotericin in a bone marrow transplant patient
Authors: Alsajri Alaa , Al-Hishma Shadan , Shubber Mazin
Published: January 2024

Introduction: Hodgkin’s lymphoma is one of the treatable types of blood
cancer. Autologous bone marrow transplantation is used as one way to
treat this disease. Neutropenic fevers are treated with antibiotics and
antifungals; including amphotericin B. Adverse effects and
hypersensitivity to Liposomal Amphotericin, Ambisome, are very few
compared to conventional amphotericin. Case report: In this case, we
noticed hypersensitivity reactions to the Ambisome, represented by an
increase in body temperature, a drop in blood pressure, hypo-oxygenemia,
in addition to an increase in the heart rate. Case management: The
patient was treated with injections of hydrocortisone, Chlorpheniramine,
and normal saline, in addition to giving oxygen, he is improved.
Conclusion: In spite of the few cases that have recorded
hypersensitivity reactions to the Ambisome, it is necessary to conduct
an allergy test and to monitor the patient well when giving the
Ambisome, especially in the first dose.
Interleukin-6 and Granulocyte colony-stimulating factor as predictors of the prognosis of influenza-associated pneumonia
Authors: Zhang Jiaying , Wang Jingxia , Gong Yiwen , Gu Yudan , Xiang Qiangqiang , Tang Ling-ling
Published: January 2024

Background: Pneumonia is a common complication of influenza and closely
related to mortality in influenza patients. Early prediction of poor
prognosis in patients with influenza-associated pneumonia is important
for reducing mortality. The present study examines cytokines as
predictors of the prognosis of influenza-associated pneumonia. Methods:
This study included 101 inpatients with influenza (64 pneumonia and 37
non-pneumonia patients). 48 cytokines were detected in the serum samples
of the patients and the clinical characteristics were analyzed. The
correlation between them was analyzed to identify predictive biomarkers
for the prognosis of influenza-associated pneumonia. Results: Seventeen
patients had poor prognosis and developed pneumonia. Among patients with
influenza-associated pneumonia, the levels of 8 cytokines were
significantly higher in those who had a poor prognosis: interleukin-6
(IL-6), interferon-γ (IFN-γ), granulocyte colony-stimulating factor
(G-CSF), monocyte colony-stimulating factor, monocyte chemoattractant
protein-1 (MCP-1), monocyte chemoattractant protein-3, Interleukin-2
receptor subunit alpha and Hepatocyte growth factor. Correlation
analysis showed that the IL-6, G-CSF, IFN-γ, and MCP-1 levels had
positive correlations with the severity of pneumonia. IL-6 and G-CSF
showed a strong and positive correlation with poor prognosis in
influenza-associated pneumonia patients. The combined effect of the two
cytokines resulted in the largest area (0.927) under the
receiver-operating characteristic curve. Conclusion: The results
indicate that the probability of poor prognosis in influenza patients
with pneumonia is significantly increased. IL-6, G-CSF, IFN-γ, and MCP-1
levels had a positive correlation with the severity of pneumonia.
Importantly, IL-6 and G-CSF were identified as significant predictors of
the severity of influenza-associated pneumonia.
The Decision Making Role of Audiological Tests in ENT Practise
Authors: Akşit Ateş Mehmet , Kösemihal Ebru
Published: January 2024

Objective This study aims to determine to what extent audiological test
data effected on decision-making in the diagnosis process of patients
who have hearing loss. Design Survey study Setting and Participants The
audiological test results of six pathologies (auditory neuropathy
spectrum disorder-ANSD, Menieré’s Disease-MD, superior semicircular
canal dehiscence-SSCD, large vestibular aquaduct syndrome-LVA, chronic
otitis media-COM, and secretory otitis media-SOM) belonging to five
different patients with a definite diagnosis were presented to 87
otolaryngology (ENT) physicians. The physicians were not informed about
patient histories and otoscopic findings. The breakdown of the
physicians according to their specialization was as follows: 19 were
residents, 53 were ENT specialists, 6 were associate professors, and 9
were professors. The participant physicians were asked to indicate
possible pathologies (one or more) for each audiological outcome. The
answers given were classified as “correct,” “undefined” and
“incorrect.” Main outcome measures To determine how effective only
audiological reports are for the diagnostic decision-making role was
evaluated by analysing the percent of “correct” answers according to
their work place and title. Results The mean “correct” response rate
for all pathologies was 19.2%, and the ” incorrect” response rate was
38%. The ratio of “undefined” answers was 41.8. The “correct”
prediction rates for each pathology, were 26% for ANSD, 32% for SOM,
19% for MD, 16% for COM and 14% for SSCD. There was no “correct”
prediction for LVA. Conclusion Audiological tests are likely to support
the decision-making process for the ENT physician when they are
accompanied by other (case history, otoscopic examination, radiological
findings etc.) The audiological report content may increase the
diagnostic value of audiological tests.
Phylogenetic analysis of porcine circovirus 3 (PCV3) circulating in Canadian pigs
Authors: Zhang Maodong , Liu Chao Chun , Huang Yanyun , Hill Janet , Araya Maria Bravo , Ojkic Davor , Gagnon C.A.
Published: January 2024

Porcine circovirus 3 (PCV3) has been detected in pigs worldwide and
associated with several clinical signs. To investigate the genetic
diversity of PCV3 strains circulating in Canada, 44 PCV3 positive
samples from Saskatchewan (2/44), Manitoba (2/44), Quebec (4/44),
Alberta (11/44) and Ontario (25/44) submitted to diagnostic laboratories
in Canada between 2019 and 2021 were sequenced and analyzed.
Phylogenetic analysis of capsid genes showed that all of the 44 Canadian
strains classified into PCV3a and segregated into seven lineages with
common amino acid changes observed at A24V, R27K, N56D, T77S, Q98R,
L150I (F) and R168K positions. Future studies are required to determine
whether the polymorphisms in capsid proteins, as revealed in this study,
could be associated with differences in the pathogenicity or
antigenicity of PCV3 strains. This is the first phylogenetic analysis of
PCV3 strains among different provinces in Canada.
Primary Debulking Surgery (PDS) is Preferable to Interval Debulking Surgery (IDS) in Advanced Ovarian Cancer when High Complete Cytoreduction Rates (R0) are Achieved: a retrospective cohort study
Authors: Barakat Anas , Ismail Aemn , Davies Quentin , Chattopadhyay Supratik
Published: January 2024

Objective: To compare the survival rates in advanced ovarian cancer
patients undergoing Primary Debulking Surgery (PDS) and Interval
Debulking Surgery (IDS). Moreover, to compare the different radical
procedures performed and perioperative morbidities in advanced ovarian
cancer patients in both PDS and IDS groups. Design: Retrospective cohort
study. Setting: University Hospitals of Leicester (UHL). Population: 102
advanced ovarian cancer patients. Methods: From the UHL register, we
identified 102 advanced ovarian cancer patients that underwent
cytoreductive surgery from January 1, 2015-January 1-2020. The
demographic criteria, radical procedures, perioperative morbidities and
survival rates were analysed and compared. Main outcome measures:
1-year, 5-year overall survival, 1-year and 5-year progression free.
Results: The 1-year and 5-year overall survival rates in FIGO stage 3c
advanced ovarian cancer patients were significantly higher in the PDS
group (92.3% and 38.6%) in contrast to IDS patients (91.2% and
67.5%). The 1-year and 5-year progression free survival rates of stage
3c and 4 were significantly higher in patients who underwent PDS (77.8%
and 26.9%) in comparison to patients who underwent IDS (61.4%, and
13.2%). Surgical complexity was higher in PDS patients; however, the
perioperative morbidities were acceptable. Conclusions: PDS was
associated with higher survival rates than IDS in advanced ovarian
cancer patients when high complete cytoreductive rates are achieved
despite being associated with higher surgical complexity. Advanced
ovarian cancer patients should be offered PDS instead of NACT if fit for
surgery. Funding: None Tweetable Abstract: PDS should be offered to
advanced ovarian cancer patients if fit for surgery.
Case Report of a Pediatric Undifferentiated Pleomorphic Sarcoma of the Cecum
Authors: Condon Kathleen , McCombs Desiree , Roybal Jessica , Warrier Raj , Falcon Corey
Published: January 2024

Undifferentiated pleomorphic sarcoma (UPS) is a high grade neoplasm
typically diagnosed in older adults and localized to the extremities or
retroperitoneum. Poor response to therapy with high rates of recurrence
confer this neoplasm a poor prognosis. This report of cecal UPS in a
12-year-old is rare due to the patient’s age and tumor location. Only
two other cases of pediatric UPS located in the intestinal tract have
been reported.
A unique case of Takotsubo cardiomyopathy following a simple partial seizure
Authors: Allam Chadi , Saouma Marc , Helou Michel Chedid El , Kadri Zeina , Badaoui Georges
Published: January 2024

A 67-year-old woman presented to the emergency department for a simple
partial seizure of her left upper and lower limbs that lasted for 1 hour
and ultimately resolved before her presentation. She has no history of
coronary artery disease, and her neurological exam was normal. Five
hours later, she complained of chest pain, and an electrocardiogram
showed ST-segment elevation in the lateral leads, and her troponin level
was increased. We diagnosed her with Takotsubo cardiomyopathy. This case
reflects the brain-heart connection and is the first reported case of
Takotsubo cardiomyopathy following a simple partial seizure.
Efficacy of cytomegalovirus specific immunoglobulins (CMV-Ig) to reduce CMV reactivation in paediatric haematopoietic stem cell transplant recipients
Authors: Geurten Claire , Ghinai Rosanna , Munford Haydn , Lawson Sarah
Published: January 2024

Cytomegalovirus (CMV) infection is a serious complication of paediatric
hematopoietic stem cell transplant (HSCT). To date, antiviral therapy
has been the mainstay of prophylaxis, with conflicting results regarding
benefits of CMV-specific immunoglobulins (CMV-Ig). After introducing
prophylactic CMV-Ig for HSCT recipients at risk (seropositive recipient
and/or donor), we conducted a single center retrospective study
comparing incidence and severity of CMV infection with and without
CMV-Ig. We identified 49 ‘at risk’ recipients from 76 consecutive HSCTs
over 3.5 years: in addition to standard antiviral prophylaxis, 10
patients received CMV-Ig and 39 did not. There was no significant
difference in donor type, cell source, conditioning or CMV status
between the groups. We observed a trend towards lower incidence of CMV
reactivation with CMV-Ig (30%) vs without (38.4%). No patient who
received CMV-Ig developed symptomatic or lethal infection. Duration of
infection appeared shorter (21 (+/-7) vs 51.4 (+/- 55) days) and peak
titers lower (4 578 (+/- 4 788) vs 24 131 (+/- 49 257)) with CMV-Ig. No
adverse events were noted. The statistical significance of results is
limited by the small sample size. These data raise interest in
prophylactic CMV-Ig as a safe way of potentially reducing severity and
duration of CMV reactivation in HSCT.
Initial Experience of Left Bundle Branch Pacing using the Abbott Agilis HisPro Catheter with stylet-driven leads
Authors: Tay Julian Cheong Kiat , Lim Eric Tien Siang , Sim Evan Yih Shan , Chua Kelvin Chi Ming , Teo Hooi Khee , Lim Paul Chun Yih , Chong Daniel Thuan Tee , Ho Kah Leng , Ching Chi Keong , Tan Boon Yew
Published: January 2024

Aims Conduction system pacing has gained steady interest over recent
years. While the majority of tools and delivery techniques were
developed for His bundle pacing (HBP), the feasibility and
reproducibility of using these similar tools for left bundle branch
pacing (LBBP) has yet to be determined. We describe our technique for
performing LBBP using the Abbott Agilis HisProTM Steerable Catheter.
Methods and results A series of 22 patients with a mean age of 71.7
years (16 males, 72.7%), underwent LBBP procedure with this catheter
between May and October 2021. Nineteen patients (86%) had successful
LBBP lead implantation. There were no major complications or mortality.
Conclusion The Agilis HisProTM catheter along with the stylet driven
Tendril STS Model 2088TC lead is a safe and feasible delivery system for
LBBP.
THE EFFECT OF PREOPERATIVE ANXIETY ON DEPTH OF ANESTHESIA AND IN VITRO FERTILIZATION SUCCESS
Authors: Sahin Sevtap Hekimoglu , Copuroglu Elif , Elter Koray , Yaman Ozge , Sut Necdet , Altinpulluk Ece Yamak
Published: January 2024

Objective The aim of this study was to evaluate the effect of
preoperative anxiety on depth of anesthesia and IVF success. Design
Population-based cohort study. Setting Trakya University Population or
Sample 131 patients between 25 and 43 years of age who were scheduled
for oocyte retrieval under sedation. Methods The patients were divided
according to Beck Anxiety Inventory (BAI): patients without anxiety were
enrolled in the low-anxious group (Group L) and patients with anxiety
were included in the high-anxious patient group (Group H). Hemodynamic
stability, fertilization rate and good quality embryo (GQE) rate were
recorded. Total propofol and fentanyl consumption was assessed
intraoperatively. Main Outcome Measures Depth of anesthesia and IVF
success Results The fertilization rate and GQE rate were not significant
between the group L and group H. Total propofol consumption was
significantly higher in group H than in groups L. The Heart rate (HR) at
preoperative and postoperatively and systolic arterial pressure (SAP) at
preoperatve and diastolic arterial pressure (DAP) at postoperative were
significantly increased in groups H than in group L. The time for
Modified Aldrete score (MAS) to reach 9 was significantly increased in
group H than in group L. The effect of variables that were found
significantly in the univariate analysis (Propofol, HRpreop, HRpostop,
SAPpreop, DAPpostop, and MAS reach of time 9) on BAI score. Conclusions
Total propofol consumption was higher in the high level of anxiety
patients, but it was not have negative effect on IVF success. Funding
Project by the Trakya University
Risk factors for catheter-related infection in children with cancer: a systematic review and meta-analysis
Authors: Rabelo Bruna , Alvarenga Kevin de , Cancela Camila , Romanelli Roberta , Rorigues Karla Emília
Published: January 2024

Rationale: To understand the risk factors for catheter-related infection
during treatment of pediatric cancer is essential to implement measures
to prevent its occurrence. Methods: We performed a systematic review of
the literature with meta-analysis to identify and synthesize the main
risk factors for catheter-related infection in children with cancer.
Systematic searches were conducted in Medline, Embase, Lilacs, and BVS.
Results: Thirteen studies were included. Risk factors were divided into
host-related, assistance-related, and catheter types. Diagnosis of
hematological neoplasm, the intensity of treatment, blood transfusion in
the 4-7 days before the infection, type of long-term catheters,
inpatient treatment, and a longer period of hospitalization were the
most consistent risk factors. Conclusions: Education for preventive
measures, reduction of hospitalization, wisely choosing the most
adequate type of catheter, and the best moment for catheter insertion
may reduce the occurrence of catheter-related infection.
Improvement in Non-Pulmonary CFTR-Related Symptoms in a Patient With p.Phe508del/p.Arg117His (7T) Cystic Fibrosis Treated with Ivacaftor
Authors: Kuek Stephanie , Massie John
Published: January 2024

Introduction: Diagnosis and management of CRMS/CFSPID and cystic
fibrosis (CF) with mild phenotypes remains challenging, and this extends
to expanding practice with the use of CFTR modulators. Case: We describe
a case of an 18-year-old man with p.F508del/p.Arg117His(7T) initially
presenting with CRMS/CFSPID. He went on to be diagnosed with pancreatic
sufficient CF with minimal lung disease and no bronchiectasis. However,
he has had significant CFTR-related symptoms with recurrent pancreatitis
and chronic sinusitis. These non-pulmonary symptoms resolved following
introduction of the CFTR modulator ivacaftor. Discussion/ Conclusion:
Diagnosis and follow up of CRMS/CFSPID infants remains challenging, with
most guidelines based on consensus opinion. Care for those with mild CF
phenotypes, CRMS/CFSPID and those with CFTR-RD must be individualised,
and open dialogue, education and patient centred care is necessary to
ascertaining which patients might benefit from management in a
multidisciplinary CF clinic and treatment. There may be a role for
expanding the use of CFTR modulators to include non-pulmonary
manifestations of CFTR dysfunction in some cases.
Deep Learning Analysis of Facial Thermal Videos for Deception Detection
Authors: Safari Iman , Derakhshan Amin , Khalilzadeh Mohammad Ali
Published: January 2024

Deception detectors are used as aids in crime detection and military
security systems. Artificial intelligence has been used to classify
deceptive and truthful messages using physiological parameters recorded
from individuals. This study aims to utilize deep learning networks to
detect deception using the extracted temperature signal on the face.
Facial thermal data sets of 32 subjects were recorded. Data sets in the
time of a mock crime scenario in two groups of deceptive and truthful
using the control question test were divided. The five temperature
signals were extracted from thermal videos by averaging the 30% of the
maximum and minimum of the region of interests (periorbital, forehead,
cheeks, perinasal, chin). This signal was used as the input of the deep
learning network. The long short-term memory network was used to
classify the extracted signals. We evaluated the network performance
using sensitivity, specificity, and accuracy criterias. The obtained
accuracy value was 93.75%, specificity 96.87%, sensitivity 90.63%
which shows notable results compared to previous research.
Comparison of the safety between conization and hysterectomy for patients with cervical adenocarcinoma in situ
Authors: Liu Jingjing , Wang Yu , wan xiaoyun , Zou Jian , Zhu Yedan , Zou Jiashan , Lu Weiguo , Xie Xing , Shen yuanming
Published: January 2024

Objective: To compare the safety between conization alone and
hysterectomy for patients with adenocarcinoma in situ (AIS) of the
cervix. Design: A cohort study of AIS patients during 2007-2021.
Setting: Women’s Hospital of Zhejiang University School of Medicine.
Population: A total of 453 AIS patients diagnosed by conization who
underwent cervical conizations only (n=153) or conization followed by
hysterectomy(n=300). Methods: The clinicopathological and follow-up data
were reviewed. Univariate analysis was examined by chi-square test and
multivariate analysis was performed by logistic regression analysis.
Main outcome measure: The rates of residual disease in specimens of
hysterectomy and repeated conization were compared between positive and
negative margin of conization. And the rates of recurrence were compared
between patients treated by conization(s) alone and hysterectomy. The
factors influencing residual disease and recurrence were assessed,
respectively. Results: Among 310 specimens of hysterectomy or repeated
conization, the rates of residual disease were 50.56% (45/89) for
positive margin and 2.26% (5/221) for negative margin, with a
significant difference (p=0.000). Totally 4 patients recurred as vaginal
intraepithelial neoplasia（VAIN）in those treated by hysterectomy and 1
recurred as cervical squamous intraepithelial neoplasia (CIN) in those
treated by conization(s) alone. The rates of recurrence were 0.65%
(1/153) for conization(s) alone and 1.33 % (4/300) for hysterectomy,
with no significant difference (p=0.431). Hysterectomy was a factor
influencing neither residual disease nor recurrence. Conclusions:
Conization is an effective and safe option for patients with AIS of the
cervix, provided the margin is negative. Keywords: adenocarcinoma in
situ, conization, hysterectomy, margins.
A rare intrapericardial cyst resected by video-assisted thoracoscopic surgery：a case report.
Authors: Li Xiong , Huang Weijiang , Liao Zhimin , Zheng Wei , Peng Baoming
Published: January 2024

Background: Pericardial cyst is a common lesion of the
mediastinum. Most of them are located outside the pericardial space,
such as in the diaphragmatic angle. However, Intrapericardial cyst is
exceedingly rare, especially in symptomatic cases, which usually require
surgical resection. To the best of our knowledge, this is the first case
report on minimally surgery for Intrapericardial cyst. Aim: To
Provide a new idea for minimally invasive surgical treatment of
intrapericardial cysts. Materials and Methods and Results: A
55-year-old women who has progressive chest pain and palpitations
symptomatic and diagnosed with intrapericardial cyst, and her clinical
symptoms were significantly alleviated after resected by video-assisted
thoracoscopic surgery（VATS）. Conclusion: Video-assisted
thoracoscopic surgery which is a feasible surgical method can greatly
reduce surgical trauma and accelerate postoperative recovery and can
achieve an excellent therapeutic effect.
Response of underground bud bank to degradation in a alpine meadows on the Qinghai-Tibet Plateau
Authors: Yang Jun , Zhang min , Wang tao , Liu feng , Zhao chuan
Published: January 2024

Exploring the diversity and formation mechanism of under-ground bud
banks is essential for understanding population regeneration and
community succession. However, there are few studies on the response of
bud bank size and composition to different degradation gradients in
alpine meadows. In view of this, we investigated the size and
composition of bud bank under four degradation gradients
(non-degraded:ND, lightly degraded:LD, moderately degraded:MD, and
heavily degraded:HD) on a typical alpine meadow in Tibet, China, and
analyzed the influence of soil physical and chemical properties on the
correlation of bud bank types. Our results show that in ND meadows,
rhizome buds dominate, in LD meadows, tiller buds account for a larger
proportion, and in MD meadows, root-sprouting buds dominate. The total
bud bank density decreases as the degradation gradient increases. The
density of cyperaceae buds decreased with the degree of degradation. The
density of leguminosae was insignificant in each degradation gradient.
The density of gramineae and weeds were dominant in LD and MD meadows,
respectively. Rhizome bud density was significantly positively
correlated with soil organic carbon (SOC), total nitrogen (TN), NH+ 4-
N, and NO- 3 - N (P < 0.001 for all), soil water content
(SWC), total phosphorus (TP) and available phosphorus (AP) (P
< 0.01), and negatively correlated with pH (P <
0.001). Tiller bud was significantly positively correlated with SWC and
TP(P < 0.05). Root-sprouting buds are only significantly
negatively correlated with TP(P < 0.05). Therefore, our
research shows that rhizome buds are more important in ND meadow
habitats, tiller buds are more important in LD meadow habitats, and
root-sprouting buds are more important in MD meadows. In addition,
rhizome buds have been proved to be suitable for survival in a weak acid
environment.
Phylotranscriptomics Reveal Multiple Whole-Genome Duplication Events and the Diversification History of Araceae
Authors: Zhao Lei , Yang Ying-Ying , Qu Xiao-Jian , Ma Hong , Hu Yi , Li Hong-Tao , Yi Ting Shuang , Li De-Zhu
Published: January 2024

Polyploidy, or whole genome duplication (WGD) is widely regarded as key
innovation promoting species diversification in plants. However, the
hypothesis still remains controversial. Here, we tested the hypothesis
by analyzing the association between polyploidy and the diversification
of Araceae. Using 1081 single/low copy orthologous clusters from 90
transcriptome datasets, we generated a new time-calibrated phylogeny of
Araceae. Based on Ks and gene tree methods, the special WGD event
(abbreviation: ψ) was shared by the True Araceae clade, and not shared
by Proto-Araceae and the lemnoids clade. By Analyses of time-dependent
and trait-dependent diversification models, our results verified that
the ψ WGD event was strongly associated with increases of net
diversification rates of the True Araceae clade, but did not promote the
diversification rates of Proto-Araceae and the lemnoids clade. Finally,
functional enrichments analyses revealed that some genes in related to
various binding, receptor and channel activity, DNA repair and cellular
response to stress, such as heat shock protein, ABC transporter,
Glycosyl hydrolase, RING finger, Pectin acetylesterase, Cytochrome P450
and Oxidative-stress-responsive kinase have experienced the expansions
in the True Araceae clade. The expansions of these genes may contribute
to the adaptation to the harsh tropical environment in the True Araceae
clade. In summary, our results reconstructed the phylotranscriptomic
framework of Araceae, revealed the diversification history of Araceae,
and suggested that WGD may not act as the determining factor but the
“Storage Cisterns” for species diversification.
Ground state solutions of the Kirchhoff equation with linearly bounded nonlinearities
Authors: Liu Yanyan , Zhao Leiga
Published: January 2024

In this paper, we consider the following
Schr\”{o}dinger-Kirchhoff equation $$
-\left(a+b \di_{\R^N}
|\nabla u|^2 \ d x
\right)\Delta u+V(x)u=f(x,u),
\ \hbox{in}\
\mathbb{R}^N, $$ where $N\geq 3$,
$a$ and $b$ are positive parameters. Under suitable assumptions on
$V$ and $f$ which is linearly bounded at infinity, the existence of
ground state solutions and their asymptotic behavior as
$b\to 0$ are established via variational methods. The
nonexistence of nontrivial solutions is also obtained for large $b$.