bluuebunny/crossref_metadata_2025 huggingface dataset:
Brief Exercise at Work (BE@Work): A Mixed-Methods Pilot Trial of a Workplace High-Intensity Interval Training Intervention
Authors: Burn Naomi L. , Weston Matthew , Atkinson Greg , Graham Michael , Weston Kathryn L.
Published: July 2021

Introduction:The efficacy of high-intensity interval training (HIIT) for improving markers of physical fitness and cardiometabolic health is promising. The workplace is one non-laboratory setting where the effectiveness of HIIT could be explored. The aim of this study was to undertake a mixed-methods exploratory pilot trial of a workplace HIIT intervention named Brief Exercise at Work (BE@Work).

Methods:Fifty-four healthy employees (mean ± standard deviation [SD] age 46 ± 10 years) from two workplaces in Northeast England were allocated to 8 weeks of thrice-weekly workplace HIIT based on boxing, stair climbing and stepping, comprising 4–7 60 s high-intensity intervals interspersed with 75 s rest (n= 30), or a no-intervention control (n= 24). The primary outcome was the change SD of predicted maximal oxygen consumption (VO2max). Markers of physical fitness, cardiometabolic health and mental well-being were also measured at baseline and follow-up. Participant perceptions of the intervention were explored in post-intervention focus groups (n= 9).

Results:Mean (±SD) session attendance was 82% (±15%). Mean peak heart rate across the intervention was 87% of age-predicted maximal heart rate with a within- and between-subject SD of 5.5% and 3.7%, respectively. The SD of changes in predicted VO2maxwas 6.6 mL·kg−1·min−1across both groups, which can be used to inform sample size estimations for a future full trial. The control-adjusted mean increase (95% confidence interval) in predicted VO2maxwas 3.9 (−0.2 to 8.1) mL·kg−1·min−1, corresponding to a Cohen's D of 0.47. We also observed preliminary evidence of small to moderate effects in favour of the intervention group for non-dominant leg extensor muscle power, markers of health-related quality of life, well-being and perceived stress and small to moderate effects in favour of the controls in perceived pain, physical activity and high-density lipoprotein cholesterol. During HIIT, focus group participants reported physiological responses they perceived as unpleasant or tiring (e.g., breathlessness, local muscular fatigue), but also that they felt alert and energised afterwards.

Conclusion:The findings of this exploratory pilot trial support the implementation of a definitive randomised controlled trial to quantify the effectiveness of a workplace HIIT intervention.
Age at Diagnosis and Baseline Myelomalacia Sign Predict Functional Outcome After Spinal Meningioma Surgery
Authors: Wach Johannes , Banat Mohammed , Schuss Patrick , Güresir Erdem , Vatter Hartmut , Scorzin Jasmin
Published: July 2021

Objective: Spinal meningioma (SM) accounts for 12% of all meningiomas. Clinical and immunohistochemical factors were analyzed with regard to functional outcome, surgical adverse events, and tumor recurrence.

Methods: One-hundred and twenty-three consecutive SM patients underwent surgery and were retrospectively reviewed with regard to demographic parameters, imaging features, neurological function, and immunohistochemical items. Neurological function was graded according to the Modified McCormick Scale (MMS) and dichotomized as “good (grade I + II)” and “poor (grade III–V)” function.

Results: One-hundred and fourteen (92.7%) WHO grade I and 9 (7.3%) WHO grade II SM were included in this study. Univariate analysis identified a baseline T2 hyperintensity of the spinal cord, baseline symptom duration ≥4 weeks, age ≥66 years, and dural tail sign as predictors of poor MMS. Baseline T2 hyperintensity of the spinal cord [Odds ratio (OR) = 13.3, 95% CI = 3.4–52.1, p < 0.001] and age ≥66 years (OR = 10.3, 95% CI = 2.6–41.1, p = 0.001) were independent predictors of a poor MMS grade at discharge after SM surgery in the multivariate binary logistic regression analysis. The 12- and 24-month recurrence-free survival rates were 98.7 % (1/80) and 94.7% (2/38), respectively. In those patients with tumor recurrence of the SM, highly increased MIB-1 (≥5%) labeling indices were observed.

Conclusion: Baseline T2 hyperintensity, especially in the elderly patients, is a strong predictor of poorer recovery after spinal meningioma surgery. SMs with high proliferative activity should be followed-up closely despite maximal safe resection.
Possible Therapeutic Strategy Involving the Purine Synthesis Pathway Regulated by ITK in Tongue Squamous Cell Carcinoma
Authors: Onidani Kaoru , Miura Nami , Sugiura Yuki , Abe Yuichi , Watabe Yukio , Kakuya Takanori , Mori Taisuke , Yoshimoto Seiichi , Adachi Jun , Kiyoi Takao , Kabe Yasuaki , Suematsu Makoto , Tomonaga Takeshi , Shibahara Takahiko , Honda Kazufumi
Published: July 2021

The epidermal growth factor receptor is the only available tyrosine kinase molecular target for treating oral cancer. To improve the prognosis of tongue squamous cell carcinoma (TSCC) patients, a novel molecular target for tyrosine kinases is thus needed. We examined the expression of interleukin-2–inducible T-cell kinase (ITK) using immunohistochemistry, and the biological function of ITK was investigated using biochemical, phosphoproteomic, and metabolomic analyses. We found that ITK is overexpressed in TSCC patients with poor outcomes. The proliferation of oral cancer cell lines expressing ITK via transfection exhibited significant increases in three-dimensional culture assays and murine inoculation models with athymic male nude mice as compared with mock control cells. Suppressing the kinase activity using chemical inhibitors significantly reduced the increase in cell growth induced by ITK expression. Phosphoproteomic analyses revealed that ITK expression triggered phosphorylation of a novel tyrosine residue in trifunctional purine biosynthetic protein adenosine-3, an enzyme in the purine biosynthesis pathway. A significant increase in de novo biosynthesis of purines was observed in cells expressing ITK, which was abolished by the ITK inhibitor. ITK thus represents a potentially useful target for treating TSCC through modulation of purine biosynthesis.
Decompose Boolean Matrices with Correlation Clustering
Authors: Aszalós László
Published: July 2021

One of the tasks of data science is the decomposition of large matrices in order to understand their structures. A special case of this is when we decompose relations, i.e., logical matrices. In this paper, we present a method based on the similarity of rows and columns, which uses correlation clustering to cluster the rows and columns of the matrix, facilitating the visualization of the relation by rearranging the rows and columns. In this article, we compare our method with Gunther Schmidt’s problems and solutions. Our method produces the original solutions by selecting its parameters from a small set. However, with other parameters, it provides solutions with even lower entropy.
FEHCA: A Fault-Tolerant Energy-Efficient Hierarchical Clustering Algorithm for Wireless Sensor Networks
Authors: Choudhary Ankur , Kumar Santosh , Gupta Sharad , Gong Mingwei , Mahanti Aniket
Published: July 2021

Technological advancements have led to increased confidence in the design of large-scale wireless networks that comprise small energy constraint devices. Despite the boost in technological advancements, energy dissipation and fault tolerance are amongst the key deciding factors while designing and deploying wireless sensor networks. This paper proposes a Fault-tolerant Energy-efficient Hierarchical Clustering Algorithm (FEHCA) for wireless sensor networks (WSNs), which demonstrates energy-efficient clustering and fault-tolerant operation of cluster heads (CHs). It treats CHs as no special node but equally prone to faults as normal sensing nodes of the cluster. The proposed scheme addresses some of the limitations of prominent hierarchical clustering algorithms, such as the randomized election of the cluster heads after each round, which results in significant energy dissipation; non-consideration of the residual energy of the sensing nodes while selecting cluster heads, etc. It utilizes the capability of vector quantization to partition the deployed sensors into an optimal number of clusters and ensures that almost the entire area to be monitored is alive for most of the network’s lifetime. This supports better decision-making compared to decisions made on the basis of limited area sensing data after a few rounds of communication. The scheme is implemented for both friendly as well as hostile deployments. The simulation results are encouraging and validate the proposed algorithm.
Modelling and Experimental Validation of a Hybrid Electric Propulsion System for Light Aircraft and Unmanned Aerial Vehicles
Authors: Cardone Massimo , Gargiulo Bonaventura , Fornaro Enrico
Published: July 2021

This article presents a numerical model of an aeronautical hybrid electric propulsion system (HEPS) based on an energy method. This model is designed for HEPS with a total power of 100 kW in a parallel configuration intended for ultralight aircraft and unmanned aerial vehicles (UAV). The model involves the interaction between the internal combustion engine (ICE), the electric motor (EM), the lithium battery and the aircraft propeller. This paper also describes an experimental setup that can reproduce some flight phases, or entire missions, for the reference aircraft class. The experimental data, obtained by reproducing two different take-offs, were used for model validation. The model can also simulate anomalous operating conditions. Therefore, the tests chosen for the model validation are characterized by the EM flux weakening (“de-fluxing”). This model is particularly suitable for preliminary stages of design when it is necessary to characterize the hybrid system architecture. Moreover, this model helps with the choice of the main components (e.g., ICE, EM, and transmission gear ratio). The results of the investigation conducted for different battery voltages and EM transmission ratios are shown for the same mission. Despite the highly simplified model, the average margin of error between the experimental and simulated results was generally under 5%.
Intelligent Transition Control between Grid-Connected and Standalone Modes of Three-Phase Grid-Integrated Distributed Generation Systems
Authors: Khan Mohammed Ali , Haque Ahteshamul , Blaabjerg Frede , Kurukuru Varaha Satya Bharath , Wang Huai
Published: July 2021

This paper proposes an intelligent seamless transition controller for smooth transition between grid-connected (GC) and standalone modes of distributed generation (DG) units in the grid. The development of this seamless controller contributes to two main processes in the transition modes: the synchronization process and an islanding process. For the synchronization process, the stationary reference frame phase-locked loop (SRF-PLL) associated with the voltage source inverter (VSI) is modified using the frequency, voltage deviation, and phase angle information. Furthermore, the islanding process is classified as intentional and unintentional islanding scenarios for achieving efficient transition control. Here, the intentional islanding process is achieved with the information that is available in the system due to the planned disconnection. For the unintentional islanding process, a fuzzy inference system (FIS) is used to modify the conventional droop control using the information of change in active power, voltage, and frequency. To identify the action of the proposed approach during the transition process, numerical simulations are conducted with the hardware-in-loop (HIL) simulator by developing a 10kWp three-phase grid-connected DG system. The results identified the efficient control of the VSI for both islanding and grid connection processes. In the islanding conditions, the proposed controller provides advantage with less detection and disconnection time, and during synchronization, it instantly minimizes the phase-angle deviation to achieve efficient control.
Experimental Investigations on Steam Generation in Nanofluids under Concentrated Solar Radiation
Authors: Jin Xin , Lin Guiping , Jin Haichuan
Published: July 2021

Developing renewable energy, especially solar energy related, is of great importance for securing our future energy society. Steam generation in nanofluids based on solar radiation has been increasingly studied. It has been determined that the efficiency of steam generation is significantly enhanced when nanoparticles are seeded into the fluid owing to their unique radiative heat transfer performance. The nanoparticles trap solar energy inside the fluid and convert it into thermal form, which dramatically accelerates the steam generation process. In this study, we experimentally investigated different nanofluids that directly absorb solar energy to generate steam. Ag nanofluid, Au nanofluid and MWCNT nanofluid with different concentration have been carefully investigated. We analyzed the temperature increase and steam generation combined with the calculation of the efficiency factor from radiative heat transfer. The heating power and steam generation power of different nanofluids and the same nanofluid with different concentrations were compared. For Au nanofluid with concentration of 0.5 wt‰, the absorbed solar energy for heating the volume and generating steam is 6 and 40 times higher than those of pure water, respectively. We concluded that localized boiling generates steam rapidly in nanofluids based on the observation of three types of nanofluids. Furthermore, the heating power and steam generation power of different nanofluids increase with concentration. Moreover, the difference between the efficiency factors results in varied volume heating and steam generation efficiencies for different nanofluids despite identical concentrations.
Study on the Enhanced Shelf Lifetime of CYTOP-Encapsulated Organic Solar Cells
Authors: Kim Jaehoon , Song Hyung-Jun , Lee Changhee
Published: July 2021

Organic solar cells (OSCs) are an attractive technique for next-generation renewable energy. However, the intrinsically unstable nature of the organic compounds involved is delaying their commercialization. Therefore, it is essential to improve the lifetime of OSCs significantly. Here, we investigated the effect of the hydrophobic cyclized transparent optical polymer (CYTOP) as a solution-processable encapsulation layer based on shelf lifetime measurement, current–voltage characteristics, and impedance spectroscopy. We found that CYTOP utilization greatly enhanced OSCs’ shelf lifetime, maintaining 96% of initial performance when unencapsulated devices decreased to 82%. Furthermore, based on the dark current characteristics, ideality factor (n), and Cole–Cole plots, the CYTOP encapsulation is revealed to effectively inhibit unfavorable changes of parasitic resistive components and trap-assisted recombination. These findings provide an inclusive perspective on the shelf lifetime issue and commercialization of the OSCs.
A Test of the Relationship between Sap Flow and Evapotranspiration, Normalized via Leaf Area, under Non-Limiting Soil Moisture
Authors: Forster Michael A.
Published: July 2021

Sap flow is the movement of fluid within plants, whereas reference evapotranspiration (ETo) occurs external to plants as the transfer of water vapor from a hypothetical grass crop. Yet, on daily time scales, and when soil moisture is non-limiting, sap flow has a positive linear relationship with ETo. Furthermore, the E2.88 model hypothesises that sap flow (Q) is equal to ETo when parameters are normalized by leaf area (AL) via the following relationship: Q/AL = ETo/2.88. The value of 2.88 is the supposed leaf area index of the hypothetical grass in the ETo model. Therefore, the E2.88 model potentially provides a null or expected value of sap flow based on independent ETo parameters and leaf area. A test of the E2.88 model was conducted via three sap flow methods (dual method approach [DMA], heat ratio [HRM], and Tmax method) on the measurement of three woody species: Pyrus communis L. (var. Beurre Bosc Pear), Syzygium floribundum F. Muell. (Weeping Lilly Pilly), and Syzygium paniculatum Gaertn. (Lilly Pilly). A data compilation of the literature expanded the sample size to include additional species. The measured trees and data compilation found a strong, positive correlation between sap flow and ETo normalized by leaf area. However, the interpretation of the results was dependent on the sap flow method. The DMA had an average accuracy of 1.6%, whereas the HRM and Tmax significantly underestimated and overestimated sap flow, respectively. This study suggested that sap flow can be reliably estimated from accurate leaf area and ETo measurements and when other variables, such as soil moisture, are non-limiting.
Networked Unmanned Aerial Vehicles for Surveillance and Monitoring: A Survey
Authors: Li Xiaohui , Savkin Andrey V.
Published: July 2021

As a typical cyber-physical system, networked unmanned aerial vehicles (UAVs) have received much attention in recent years. Emerging communication technologies and high-performance control methods enable networked UAVs to operate as aerial sensor networks to collect more complete and consistent information with significantly improved mobility and flexibility than traditional sensing platforms. One of the main applications of networked UAVs is surveillance and monitoring, which constitute essential components of a well-functioning public safety system and many industrial applications. Although the existing literature on surveillance and monitoring UAVs is extensive, a comprehensive survey on this topic is lacking. This article classifies publications on networked UAVs for surveillance and monitoring using the targets of interest and analyzes several typical problems on this topic, including the control, navigation, and deployment optimization of UAVs. The related research gaps and future directions are also presented.
Remote Work, Work Stress, and Work–Life during Pandemic Times: A Latin America Situation
Authors: Sandoval-Reyes Juan , Idrovo-Carlier Sandra , Duque-Oliva Edison Jair
Published: July 2021

The COVID-19 pandemic affected the relationship between work and life almost everywhere on the planet. Suddenly, remote work became the mainstream way of working for millions of workers. In this context, we explore how the relationship between remote work, work stress, and work–life developed during pandemic times in a Latin America context. In a sample of 1285 responses collected between April and May 2020, through a PLS-SEM model, we found that remote work in pandemic times increased perceived stress (β = 0.269; p < 0.01), reduced work–life balance (β = −0.225; p < 0.01) and work satisfaction (β = −0.190; p < 0.01), and increased productivity (β = 0.120; p < 0.01) and engagement (β = 0.120; p < 0.01). We also found a partial moderating effect, competitive and complementary, of perceived stress, and one significant gender difference: when working remotely, perceived stress affects men’s productivity more acutely than women’s productivity.
“If It Goes Horribly Wrong the Whole World Descends on You”: The Influence of Fear, Vulnerability, and Powerlessness on Police Officers’ Response to Victims of Head Injury in Domestic Violence
Authors: Richards Jenny , Smithson Janet , Moberly Nicholas J. , Smith Alicia
Published: July 2021

Domestic violence (DV) victims face significant barriers to accessing healthcare. This is particularly concerning in cases of brain injury (BI), which is difficult to diagnose and risks severe long-term consequences for DV victims. Police may be able to identify head injury (HI) and signpost victims to healthcare. This research investigated potential barriers to police supporting victim health needs by exploring police attitudes towards DV and considering how police interpret and respond to stories of HI in DV victims. Individual interviews were conducted with 12 police officers from forces in South and Central England. This included the use of a clinical vignette. Thematic analysis highlighted three global themes: ‘seesaw of emotions’, ‘police vulnerability’, and ‘head injury is fearful’. Police officers’ vulnerability to external blame was the predominant influence in their responses to HI.
Clustering of Lifestyle Risk Factors among Algerian Adolescents: Comparison between Urban and Rural Areas: GSHS Data
Authors: Kerkadi Abdelhamid , Al Mannai Hissa , Saad Dana , Yakti Fatima al Zahra , Attieh Grace , Bawadi Hiba
Published: July 2021

Objective: Compare the clustering of LBRs between urban and rural Algerian adolescents. Design: Data of this cross-sectional study was derived from the Global School-based Health Survey (GSHS). A self-administered, anonymous questionnaire was filled out by 4532 adolescents (11–16 years), which addressed LBRs of NCDs. Life style behavioral risk factors (LBRs) clustering was measured by the ratios of observed (O) and expected (E) prevalence of one or more simultaneously occurring LBRs for urban and rural areas separately. Multivariate logistic regression was performed to examine the association of LBRs as dependent variable with demographic variables (location, age, gender). Results: The most common LBR was physical inactivity (84.6%: 50.9% for urban and 49.1% for rural). Adolescents in urban areas had a higher prevalence of two (56.8% vs. 43.2%) and three and more (61.3% vs. 38.7%) LBRs than in rural areas. In urban areas, a significant positive association was found between (low fruit and vegetable consumption + physical inactivity) [2.06 (1.61–2.64)] and (high SB + smoking) [2.10 (1.54–2.76)], while (physical inactivity + high SB) [0.70 (0.54–0.91)] showed a significant negative association. In rural areas, (high SB + overweight/obesity) [1.49 (1.09–2.04)] had a significant positive association. While, (low fruit and vegetable consumption + high SB) [0.75 (0.60–0.94)], (physical inactivity + high SB) [0.65 (0.49–0.86)], and (physical inactivity + smoking) [0.70 (0.49–0.99)] had a negative association. Conclusions: Several socio-demographic factors have been identified to play a role in LBRs clustering among Algerian adolescents. Results of the study suggest the development of intervention aiming to tackle different LBRs rather than focusing on a single LBR.
A Childhood Farm Environment Protects from Allergic Sensitization until Middle Age but Not from New-Onset Sensitization in Adulthood: A 15 Year Longitudinal Study
Authors: Haarala Anna Karoliina , Sinikumpu Suvi-Päivikki , Vaaramo Eeva , Jokelainen Jari , Timonen Markku , Auvinen Juha , Pekkanen Juha , Huilaja Laura
Published: July 2021

Data are insufficient on the protective effect of a farm environment in childhood regarding sensitization in middle age and new-onset sensitization in adulthood. A skin prick test (SPT) and questionnaire data from the Northern Finland Birth Cohort 1966 study (NFBC66) were used to investigate sensitization at age 46 years related to childhood living environment. A subpopulation of 3409 participants was analyzed to study factors related to new-onset sensitization between ages of 31 and 46 years. Data on complete SPTs were available for 5373 cohort members at age 46. Professional farming by parents (odds ratio (OR) 0.54; 95% confidence interval (CI) 0.43–0.68) and keeping of farm animals (OR 0.53; 95% CI 0.43–0.66) in infancy were associated with a lower risk of sensitization at age 46. Sensitization (OR 0.58; 95% CI 0.47–0.72) and polysensitization (OR 0.43; 95% CI 0.32–0.57) were less common in those who lived in a rural area in infancy compared to a city area. The childhood living environment had no effect on new-onset sensitization between ages 31 and 46. We conclude that living on a farm or in a rural environment in childhood had a protective effect on sensitization even in middle age, but these factors did not protect from new-onset sensitization in adults.
Total Sleep Time in the Taiwan Obstructive Lung Disease Cohort
Authors: Chuang Li-Pang , Hsieh Meng-Jer , Chen Ning-Hung , Hu Han-Chung , Yang Cheng-Ta , Tsai Ying-Huang , Lin Shih-Wei
Published: July 2021

Patients with chronic obstructive pulmonary disease (COPD) have been reported to have poor sleep quality. However, total sleep time has not been evaluated in detail among patients with COPD. This retrospective, observational, multicenter research study was performed across six participating hospitals in Taiwan, with a total of 421 adult patients enrolled. Pulmonary function, the Modified British Medical Research Council Dyspnea Scale, the COPD Assessment Test and basic clinical data were assessed. The Pittsburgh Sleep Quality Index was also administered to patients, and the total sleep time was extracted for further analysis. The patients whose total sleep time was between 6 and 7 h had better pulmonary function, and the patients who slept less than 5 h had worse comorbidities. There was a significant higher total sleep time in Global Initiatives for Chronic Obstructive Lung Disease (GOLD) group B compared to GOLD group A. COPD patients who sleep between 5 and 6 h used fewer oral steroids and were less likely to use triple therapy (long-acting beta-agonist, long-acting muscarinic antagonist, inhaled cortical steroid). COPD patients sleeping from 5 to 7 h had better clinical features than those sleeping less than 5 h in terms of pulmonary function, comorbidities and medication usage.
Knowledge, Attitudes and Practices toward Hepatitis B Virus Infection among Students of Medicine in Vietnam
Authors: Nguyen Thi Thuy Linh , Pham Thi Thanh Hang , So Samuel , Hoang Thi Hai Van , Nguyen Thi To Uyen , Ngo Thanh Binh , Nguyen Minh Phuong , Thai Quang Hung , Nguyen Ngoc Khoi , Le Ho Thi Quynh Anh , Tran Quang Phuc , Pham Minh Khue
Published: July 2021

Background: Building capacity in hepatitis B virus prevention and management for medical students and health professionals is one of the pillars of the national viral hepatitis control strategy. Methods: A cross-sectional study was conducted at eight medical universities from the northern, central and southern regions of the country between May and November 2020 using a systematic random sampling technique. Results: Among 2000 participants, 84.2% reported they had been tested for hepatitis B and 83.9% had received the hepatitis B vaccine. The mean knowledge, attitude, practice score was 40.2 out of 54 (74.4%) with only 19.9% of the study participants obtaining a good score. In multivariate analysis, fifth year students, students from central universities, students who had tested positive for hepatitis B and students who had received hepatitis B vaccine or had encountered patients with chronic hepatitis B had significantly higher knowledge score (p < 0.05). The study showed lack of trust in the hepatitis B vaccine safety and lack of confidence in providing counselling, testing and management of patients with chronic hepatitis B. Conclusion: Findings from our research emphasized an immediate need to improve the medical schools’ training curriculum in Vietnam to enable students’ readiness in hepatitis B prevention and management.
Association between Early Life Child Development and Family Dog Ownership: A Prospective Birth Cohort Study of the Japan Environment and Children’s Study
Authors: Minatoya Machiko , Ikeda-Araki Atsuko , Miyashita Chihiro , Itoh Sachiko , Kobayashi Sumitaka , Yamazaki Keiko , Ait Bamai Yu , Saijo Yasuaki , Sato Yukihiro , Ito Yoshiya , Kishi Reiko ,   The Japan Environment and Children’s Study Group
Published: July 2021

Pets may play a role in the social-emotional development of children. In particular, some studies have suggested that family dog ownership is associated with better health outcomes. To date, no study has assessed child development in association with dog ownership of different time points. The purpose of the current study was primary to investigate whether “ever” family dog ownership was associated with early child development, and secondary to further examine whether associations between family dog ownership and early child development differ among family dog ownership of status, including “past only”, “current only”, and “always” groups, using the data of family dog ownership obtained at multiple time points. Associations between family dog ownership and infant development at 3 years of age were examined using data from a nationwide prospective birth cohort study, the Japan Environment and Children’s Study (n = 78,941). “Ever” family dog ownership was categorized to “past only”, “current only”, and “always”. We observed that children with “ever” family dog ownership showed a significantly decreased risk of developmental delay in the communication (odds ratio [OR] = 0.87; 95% confidence interval [CI]: 0.78, 0.96), gross motor (OR = 0.83; 95% CI: 0.76, 0.92), problem-solving (OR = 0.89; 95% CI: 0.83, 0.96) and personal-social (OR = 0.86; 95% CI: 0.72, 0.92) domains compared to children with “never” family dog ownership. Furthermore, a significantly decreased risk of developmental delay in gross motor function was observed in association with living with dogs in the “past only” (OR = 0.83; 95% CI: 0.73, 0.95) and “always” (OR = 0.86; 95% CI: 0.75, 0.98). In addition, a decreased risk of developmental delay in the problem-solving domain was associated with “past” family dog ownership (OR = 0.87; 95% CI: 0.79, 0.97) and in the personal-social domain was associated with “always” family dog ownership (OR = 0.81; 95% CI = 0.68, 0.95). Given the possible positive association between early life child development and family dog ownership, living with dogs may be an important factor to be considered when assessing child development.
Study Crafting and Self-Undermining in Higher Education Students: A Weekly Diary Study on the Antecedents
Authors: Körner Lorena Sarah , Rigotti Thomas , Rieder Kerstin
Published: July 2021

The aim of the current study is to validate the adaptation of the job demands–resources theory to the study context. In addition, we introduce the concepts study crafting and self-undermining to the study demands–resources framework by examining the mediating role of engagement and exhaustion in the relationship between study characteristics and study crafting and self-undermining. Over four consecutive weeks, 205 higher education students answered a questionnaire about their weekly study demands and resources, their well-being (i.e., engagement, exhaustion), and their study crafting and self-undermining behaviors. Multilevel structural equation modeling (controlling for autoregressors of mediators and dependent variables from the previous week) demonstrated a positive relationship between study resources and study crafting mediated by engagement, as well as a positive relationship between study demands and self-undermining mediated by exhaustion. Our findings show that even short-term fluctuations in study characteristics affect students’ well-being and, in turn, their proactive and dysfunctional behaviors. Accordingly, universities should provide a resource-rich study environment and limit study demands as much as possible. Furthermore, our results demonstrate that students can also actively influence their study environment themselves.
Differences in Trajectories and Predictive Factors of Cognition over Time in a Sample of Cognitively Healthy Adults, in Zaragoza, Spain
Authors: Lobo Elena , Gracia-García Patricia , Lobo Antonio , Saz Pedro , De-la-Cámara Concepción
Published: July 2021

Great inter-individual variability has been reported in the maintenance of cognitive function in aging. We examined this heterogeneity by modeling cognitive trajectories in a population-based longitudinal study of adults aged 55+ years. We hypothesized that (1) distinct classes of cognitive trajectories would be found, and (2) between-class differences in associated factors would be observed. The sample comprised 2403 cognitively healthy individuals from the Zaragoza Dementia and Depression (ZARADEMP) project, who had at least three measurements of the Mini-Mental State Examination (MMSE) in a 12-year follow-up. Longitudinal changes in cognitive functioning were modeled using growth mixture models (GMM) in the data. The best-fitting age-adjusted model showed 3 distinct trajectories, with 1-high-to-moderate (21.2% of participants), 2-moderate-stable (67.5%) and, 3-low-and-declining (9.9%) cognitive function over time, respectively. Compared with the reference 2-trajectory, the association of education and depression was significantly different in trajectories 1 and 3. Instrumental activities of daily living (iADLs) were only associated with the declining trajectory. This suggests that intervention strategies should be tailored specifically to individuals with different trajectories of cognitive aging, and intervention strategies designed to maintain cognitive function might be different from those to prevent decline. A stable cognitive performance (‘successful cognitive aging’) rather than a mild decline, might be more ‘normal’ than generally expected.
Systemic Lupus Erythematosus Research: A Bibliometric Analysis over a 50-Year Period
Authors: Koo Malcolm
Published: July 2021

Bibliometric analysis is a well-established approach to quantitatively assess scholarly productivity. However, there have been few assessments of research productivity on systemic lupus erythematosus (SLE) to date. The aim of this study was to analyze global research productivity through original articles published in journals indexed by the Web of Science from 1971 to 2020. Bibliometric data was obtained from the Science Citation Index Expanded in the Web of Science Core Collection database. Only original articles published between 1971 and 2020 on SLE were included in the analysis. Over the 50-year period, publication production in SLE research has steadily increased with a mean annual growth rate of 8.0%. A total of 44,967 articles published in 3435 different journals were identified. The journal Lupus published the largest number of articles (n = 3371; 8.0%). A total of 148 countries and regions contributed to the articles. The global productivity ranking was led by the United States (n = 11,244, 25.0%), followed by China (n = 4893, 10.9%). A three-field plot showed that the Oklahoma Medical Research Foundation and the Johns Hopkins University together contributed 18.5% of all articles from the United States. A co-occurrence network analysis revealed five highly connected clusters of SLE research. In conclusion, this bibliometric analysis provided a comprehensive overview of the status of SLE research, which could enable a better understanding of the development in this field in the past 50 years.
Assessment and Impact of the Risk of Exposure of Portuguese Biomedical Scientists in the Context of COVID-19
Authors: Tavares Ana Sofia R. , Bellém Fernando , Abreu Renato , Leitão Céu , Medeiros Nuno , Alves Patrícia , Calmeiro Luís
Published: July 2021

Currently, the main public health concern worldwide is the COVID-19 pandemic, caused by SARS-CoV-2, which was recently discovered and described. Due to its high pathogenicity and infectiousness, it is necessary to determine the risk healthcare professionals face every day while dealing with infected patients and contaminated biological samples. The purpose of this study was to assess Portuguese Biomedical Scientists’ risk of COVID-19 exposure and related stress appraisals. One hundred and forty participants completed online versions of the WHO’s Risk Assessment and Management of Exposure Survey and the Stress Appraisal Measure. Participants worked mainly in outpatient settings (45%), and in emergency services (28%). Twenty three percent of participants were exposed to COVID-19 through community exposure, and 39% through occupational exposure. Although 95% reported using personal protective equipment (PPE), 83.6% were at high risk of infection. However, the use of some types of PPE was related to the risk of exposure. Participants reported moderate perceptions of stress and threat, but also moderate perceptions of control over the situation. These results may contribute to a more efficient risk management of these professionals and prevent disease transmission in hospitals and communities.
Gender Difference in the Relationships between Inflammatory Markers, Serum Uric Acid and Framingham Risk Score
Authors: Huang Jui-Hua , Li Ren-Hau , Huang Shu-Ling , Sia Hon-Ke , Yu Chao-Hung , Tang Feng-Cheng
Published: July 2021

The purpose of the present study was to explore the role of gender in the relation of high-sensitivity C-reactive protein (hsCRP), white blood cell (WBC) count, and serum uric acid (UA) to the risk of future cardiovascular disease (CVD) events. In total, 404 workers were recruited to obtain the measurements of serum markers for CVD risk. Demographic data, nutrition, exercise, smoking, and alcohol consumption were assessed through a questionnaire. The Framingham Risk Score (FRS) was adopted to estimate the risk of future CVD events. Multiple linear regression models were used to determine CVD risk markers in relation to the FRS by gender. The hsCRP was not significantly correlated with the FRS for all workers after adjusting for covariates, including demographic data and health-related lifestyle. WBC count was positively correlated with FRS for all workers, but WBC count did not show an interaction with gender with respect to the FRS. Serum UA showed an interaction with gender on the FRS, and UA positively correlated with the FRS in males though not in females. With respect to CVD prevention, the WBC count can be used to monitor the risk for all workers. Due to a gender difference shown in the relationship between serum UA and the FRS, serum UA can be a monitor of the risk of future CVD events in male workers only.
University Staff and Students’ Attitudes towards a Completely Smoke-Free Campus: Shifting Social Norms and Organisational Culture for Health Promotion
Authors: Sendall Marguerite C. , Fox Lauren , Wraith Darren
Published: July 2021

A large university in Queensland, Australia with a diverse staff and student community introduced a campus wide smoke-free policy in 2016. The purpose of this enquiry was to understand attitudes about a new smoke-free policy, its potential impact and the shift in social norms and organizational culture to inform the next phase of implementation. An electronic survey was distributed to all staff and students approximately 12 weeks after the smoke-free policy was implemented. The survey consisted of multiple-choice questions about demographics, smoking behaviour, attitudes towards smoking and tobacco control, awareness of the smoke-free policy, and attitudes towards the effect of a completely smoke-free campus on quality of life, learning and enrolment. The survey was completed by 641 university staff and students. Respondents reported seeking out (80.4%) and socialising in smoke-free environments (86.6%) and supported smoke-free buildings (96.1%), indoor areas (91.6%), and outdoor areas (79%). The results revealed overwhelming support for a completely smoke-free campus (83%) and minority support for designated smoking areas (31%). Overall, respondents reflected positively towards a campus wide smoke-free policy. These findings suggest Queensland’s early adoption of tobacco control laws influenced the social environment, de-normalised smoking, changed behaviour, preference for smoke-free environments and shifted social norms. These findings provide convincing evidence for organisational change and suggest health promotion policy makers should progress the implementation of smoke-free policies nationally across the higher education sector.
Extreme Enlargement of the Inverted Repeat Region in the Plastid Genomes of Diatoms from the Genus Climaconeis
Authors: Gastineau Romain , Davidovich Nikolaï A. , Davidovich Olga I. , Lemieux Claude , Turmel Monique , Wróbel Rafał J. , Witkowski Andrzej
Published: July 2021

We sequenced the plastid genomes of three diatoms from the genus Climaconeis, including two strains formerly designated as Climaconeis scalaris. At 208,097 and 216,580 bp, the plastid genomes of the latter strains are the largest ever sequenced among diatoms and their increased size is explained by the massive expansion of the inverted repeat region. Important rearrangements of gene order were identified among the two populations of Climaconeis cf. scalaris. The other sequenced Climaconeis chloroplast genome is 1.5 times smaller compared with those of the Climaconeis cf. scalaris strains and it features an usual quadripartite structure. The extensive structural changes reported here for the genus Climaconeis are compared with those previously observed for other algae and plants displaying large plastid genomes.
Muricholic Acids Promote Resistance to Hypercholesterolemia in Cholesterol-Fed Mice
Authors: Gaillard Dany , Masson David , Garo Erwan , Souidi Maamar , Pais de Barros Jean-Paul , Schoonjans Kristina , Grober Jacques , Besnard Philippe , Thomas Charles
Published: July 2021

Background and aims: Hypercholesterolemia is a major risk factor for atherosclerosis and cardiovascular diseases. Although resistant to hypercholesterolemia, the mouse is a prominent model in cardiovascular research. To assess the contribution of bile acids to this protective phenotype, we explored the impact of a 2-week-long dietary cholesterol overload on cholesterol and bile acid metabolism in mice. Methods: Bile acid, oxysterol, and cholesterol metabolism and transport were assessed by quantitative real-time PCR, western blotting, GC-MS/MS, or enzymatic assays in the liver, the gut, the kidney, as well as in the feces, the blood, and the urine. Results: Plasma triglycerides and cholesterol levels were unchanged in mice fed a cholesterol-rich diet that contained 100-fold more cholesterol than the standard diet. In the liver, oxysterol-mediated LXR activation stimulated the synthesis of bile acids and in particular increased the levels of hydrophilic muricholic acids, which in turn reduced FXR signaling, as assessed in vivo with Fxr reporter mice. Consequently, biliary and basolateral excretions of bile acids and cholesterol were increased, whereas portal uptake was reduced. Furthermore, we observed a reduction in intestinal and renal bile acid absorption. Conclusions: These coordinated events are mediated by increased muricholic acid levels which inhibit FXR signaling in favor of LXR and SREBP2 signaling to promote efficient fecal and urinary elimination of cholesterol and neo-synthesized bile acids. Therefore, our data suggest that enhancement of the hydrophilic bile acid pool following a cholesterol overload may contribute to the resistance to hypercholesterolemia in mice. This work paves the way for new therapeutic opportunities using hydrophilic bile acid supplementation to mitigate hypercholesterolemia.
Iron Chelator Induces Apoptosis in Osteosarcoma Cells by Disrupting Intracellular Iron Homeostasis and Activating the MAPK Pathway
Authors: Xue Yanru , Zhang Gejing , Zhou Shoujie , Wang Shenghang , Lv Huanhuan , Zhou Liangfu , Shang Peng
Published: July 2021

Osteosarcoma is a common malignant bone tumor in clinical orthopedics. Iron chelators have inhibitory effects on many cancers, but their effects and mechanisms in osteosarcoma are still uncertain. Our in vitro results show that deferoxamine (DFO) and deferasirox (DFX), two iron chelators, significantly inhibited the proliferation of osteosarcoma cells (MG-63, MNNG/HOS and K7M2). The viability of osteosarcoma cells was decreased by DFO and DFX in a concentration-dependent manner. DFO and DFX generated reactive oxygen species (ROS), altered iron metabolism and triggered apoptosis in osteosarcoma cells. Iron chelator-induced apoptosis was due to the activation of the MAPK signaling pathway, with increased phosphorylation levels of JNK, p38 and ERK, and ROS generation; in this process, the expression of C-caspase-3 and C-PARP increased. In an orthotopic osteosarcoma transplantation model, iron chelators (20 mg/kg every day, Ip, for 14 days) significantly inhibited the growth of the tumor. Immunohistochemical analysis showed that iron metabolism was altered, apoptosis was promoted, and malignant proliferation was reduced with iron chelators in the tumor tissues. In conclusion, we observed that iron chelators induced apoptosis in osteosarcoma by activating the ROS-related MAPK signaling pathway. Because iron is crucial for cell proliferation, iron chelators may provide a novel therapeutic strategy for osteosarcoma.
Therapeutic Perspectives of Thermogenic Adipocytes in Obesity and Related Complications
Authors: Wang Chih-Hao , Wei Yau-Huei
Published: July 2021

There is a rapidly increasing prevalence of obesity and related metabolic disorders such as type 2 diabetes worldwide. White adipose tissue (WAT) stores excess energy, whereas brown and beige adipose tissues consume energy to generate heat in the process of thermogenesis. Adaptive thermogenesis occurs in response to environmental cues as a means of generating heat by dissipating stored chemical energy. Due to its cumulative nature, very small differences in energy expenditure from adaptive thermogenesis can have a significant impact on systemic metabolism over time. Targeting brown adipose tissue (BAT) activation and converting WAT to beige fat as a method to increase energy expenditure is one of the promising strategies to combat obesity. In this review, we discuss the activation of the thermogenic process in response to physiological conditions. We highlight recent advances in harnessing the therapeutic potential of thermogenic adipocytes by genetic, pharmacological and cell-based approaches in the treatment of obesity and metabolic disorders in mice and the human.
Consequences of LED Lights on Root Morphological Traits and Compounds Accumulation in Sarcandra glabra Seedlings
Authors: Xie Dejin , Tarin Muhammad Waqqas Khan , Chen Lingyan , Ren Ke , Yang Deming , Zhou Chengcheng , Wan Jiayi , He Tianyou , Rong Jundong , Zheng Yushan
Published: July 2021

This study evaluated the effects of different light spectra (white light; WL, blue light; BL and red light; RL) on the root morphological traits and metabolites accumulation and biosynthesis in Sarcandra glabra. We performed transcriptomic and metabolomic profiling by RNA-seq and ultra-performance liquid chromatography−electrospray ionization−tandem mass spectrometry (UPLC-ESI-MS/MS), respectively. When morphological features were compared to WL, BL substantially increased under-ground fresh weight, root length, root surface area, and root volume, while RL inhibited these indices. A total of 433 metabolites were identified, of which 40, 18, and 68 compounds differentially accumulated in roots under WL (WG) vs. roots under BL (BG), WG vs. roots under RL (RG), and RG vs. BG, respectively. In addition, the contents of sinapyl alcohol, sinapic acid, fraxetin, and 6-methylcoumarin decreased significantly in BG and RG. In contrast, chlorogenic acid, rosmarinyl glucoside, quercitrin and quercetin were increased considerably in BG. Furthermore, the contents of eight terpenoids compounds significantly reduced in BG. Following transcriptomic profiling, several key genes related to biosynthesis of phenylpropanoid-derived and terpenoids metabolites were differentially expressed, such as caffeic acid 3-O-methyltransferase) (COMT), hydroxycinnamoyl-CoA shikimate hydroxycinnamoyl transferase (HCT), O-methyltransferase (OMT), and 1-deoxy-D-xylulose-5-phosphate synthetase (DXS). In summary, our findings showed that BL was suitable for growth and accumulation of bioactive metabolites in root tissue of S. glabra. Exposure to a higher ratio of BL might have the potential to improve the production and quality of S. glabra seedlings, but this needs to be confirmed further.