bluuebunny/crossref_metadata_2025 huggingface dataset:
The association between ambient temperature and sperm quality in Wuhan, China
Authors: Wang Xiaochen , Tian Xiaojia , Ye Bo , Zhang Yi , Zhang Xiaotong , Huang Shichun , Li Cunlu , Wu Simin , Li Rui , Zou Yuliang , Liao Jingling , Yang Jing , Ma Lu
Published: April 2020

Background

Few epidemiological investigations have focused on the influence of environmental temperature on human sperm quality. Here, we evaluated the potential association between ambient temperature and human sperm quality in Wuhan, China, and examined the interactive effect of particulate matter (PM2.5) and temperature.

Methods

1780 males who had been living in Wuhan for no less than three months and received semen analysis at the Department of Reproductive Medicine in Renmin Hospital of Wuhan University between April 8, 2013 and June 30, 2015 were recruited. Daily mean meteorological data and air pollution data (PM2.5, O3 and NO2) in Wuhan between 2013 and 2015 were collected. A generalized linear model was used to explore the associations between ambient temperature and sperm quality (including sperm concentration, percentage of normal sperm morphology, and progressive motility) at 0–9, 10–14, 15–69, 70–90, and 0–90 days before semen examination, and the interaction between temperature and PM2.5.

Results

The associations between ambient temperature and sperm quality were an inverted U-shape at five exposure windows, except for a lag of 0–9 days for sperm concentration. A 1 °C increase in ambient temperature above the thresholds was associated with a 2.038 (1.292 ~ 2.783), 1.814 (1.217 ~ 2.411), 1.458 (1.138 ~ 1.777), 0.934(0.617 ~ 1.251) and 1.604 (1.258 ~ 1.951) decrease in the percentage of normal sperm morphology at lag 0–9, lag 10–14, lag 15–69, lag 70–90, and lag 0–90 days, respectively. The interaction p-values of PM2.5 and temperature were mostly less than 0.05 at five exposure windows. When ambient temperature exposure levels were above the thresholds, a 0.979 (0.659–1.299) and 3.559 (0.251 ~ 6.867) decrease in percentage of normal sperm morphology per 1 °C increase in temperature at lag 0–90 days was observed in the PM2.5 ≤ P50 group and PM2.5 > P50 group, respectively.

Conclusions

Our results indicate that exposure to ambient temperature has a threshold effect on sperm quality, and PM2.5 enhances the effect of temperature on sperm quality when temperatures are above the threshold.
RETRACTED ARTICLE: M2 Macrophagy-derived exosomal miRNA-5106 induces bone mesenchymal stem cells towards osteoblastic fate by targeting salt-inducible kinase 2 and 3
Authors: Xiong Yuan , Chen Lang , Yan Chenchen , Zhou Wu , Yu Tao , Sun Yun , Cao Faqi , Xue Hang , Hu Yiqiang , Chen Dong , Mi Bobin , Liu Guohui
Published: April 2020

Background

Osteoblast differentiation is a vital process for fracture healing, and exosomes are nanosized membrane vesicles that can deliver therapeutic drugs easily and safely. Macrophages participate in the regulation of various biological processes in vivo, and macrophage-derived exosomes (MD-Exos) have recently been a topic of increasing research interest. However, few study has explored the link between MD-Exos and osteoblast differentiation. Herein, we sought to identify miRNAs differentially expressed between M1 and M2 macrophage-derived exosomes, and to evaluate their roles in the context of osteoblast differentiation.

Results

We found that microRNA-5106 (miR-5106) was significantly overexpressed in M2 macrophage-derived exosomes (M2D-Exos), while its expression was decreased in M1 macrophage-derived exosomes (M1D-Exos), and we found that this exosomal miRNA can induce bone mesenchymal stem cell (BMSC) osteogenic differentiation via directly targeting the Salt-inducible kinase 2 and 3 (SIK2 and SIK3) genes. In addition, the local injection of both a miR-5106 agonist or M2D-Exos to fracture sites was sufficient to accelerate healing in vivo.

Conclusions

Our study demonstrates that miR-5106 is highly enriched in M2D-Exos, and that it can be transferred to BMSCs wherein it targets SIK2 and SIK3 genes to promote osteoblast differentiation.
Influence of schooling on the health-related quality of life of children with rare diseases
Authors: Paz-Lourido Berta , Negre Francisca , de la Iglesia Begoña , Verger Sebastià
Published: April 2020

Background

Although participation of children with rare diseases in school is considered beneficial, it poses new challenges for the educational system, but also for the affected children and their families. The aim of this study is to identify which aspects of the schooling experience may have an impact on the health-related quality of life of children with rare diseases.

Method

A qualitative study was conducted using the social-critical paradigm as theoretical perspective. Participants (n = 28) included children with rare diseases (n = 8), parents (n = 12) and school staff (n = 8). Data was obtained through in-depth interviews and focus groups and analysed through discourse analysis as methodological orientation.

Results

Participants’ discourses placed value on the social benefits of inclusion of children with rare diseases in schooling. Discourses also highlighted how the low numbers of children with rare diseases and the delay, or lack, of a clear diagnosis are among the difficulties experienced in the pursuit of the adaptations that children and teachers need to promote a healthy and normalized school experience. The issues identified in their health-related quality of life were summarized in seven categories: Attendance, Knowledge, Participation, Acceptance, Discrimination, Safety, Health-Related Support.

Conclusion

Children with rare diseases remain, in many cases, invisible at the educational level due to the low numbers of children affected, limiting the kind of resources available to the child and teaching staff. This situation requires inter-disciplinary and inter-sector measures between health services and educational environments to articulate a comprehensive approach focused on children’s clinical needs.
How personalised medicine will transform healthcare by 2030: the ICPerMed vision
Authors: Vicente Astrid M. , Ballensiefen Wolfgang , Jönsson Jan-Ingvar
Published: April 2020

This commentary presents the vision of the International Consortium for Personalised Medicine (ICPerMed) on how personalised medicine (PM) will lead to the next generation of healthcare by 2030. This vision focuses on five perspectives: individual and public engagement, involvement of health professionals, implementation within healthcare systems, health-related data, and the development of sustainable economic models that allow improved therapy, diagnostic and preventive approaches as new healthcare concepts for the benefit of the public. We further identify four pillars representing transversal issues that are crucial for the successful implementation of PM in all perspectives. The implementation of PM will result in more efficient and equitable healthcare, access to modern healthcare methods, and improved control by individuals of their own health data, as well as economic development in the health sector.
The SENIEUR protocol and the efficacy of hepatitis B vaccination in healthy elderly persons by age, gender, and vaccine route
Authors: Edelman Robert , Deming Meagan E. , Toapanta Franklin R. , Heuser Mark D. , Chrisley Lisa , Barnes Robin S. , Wasserman Steven S. , Blackwelder William C. , Handwerger Barry S. , Pasetti Marcela , Siddiqui Khan M. , Sztein Marcelo B.
Published: April 2020

Background

Reduced response to hepatitis B vaccines is associated with aging, confounding and comorbid conditions, as well as inadvertent subcutaneous (SC) inoculation. We hypothesized that the antibody and T cell-mediated immune responses (T-CMI) of elderly adults to a vaccine intended for intramuscular (IM) administration would be attenuated when deposited into SC fat, independent of confounding conditions.

Results

Fifty-two healthy, community dwelling elderly adults (65–82 years), seronegative for HBV, were enrolled in the SENIEUR protocol as a strictly healthy population. These seniors were randomized to receive a licensed alum-adjuvanted recombinant HBV vaccine either SC or IM, with the inoculum site verified by imaging. The response rates, defined as hepatitis B surface antibodies (HBsAb) ≥10 IU/L, were significantly lower in the elderly than in young adults, a group of 12, healthy, 21–34-year-old volunteers. Moreover, elderly participants who received the vaccine IM were significantly more likely to be responders than those immunized SC (54% versus 16%, p = 0.008). The low seroconversion rate in the IM group progressively declined with increasing age, and responders had significantly lower HBsAb titers and limited isotype responses. Moreover, T-CMI (proliferation and cytokine production) were significantly reduced in both percentage of responders and intensity of the response for both Th1 and Th2 subsets in the elderly.

Conclusions

Our data demonstrate the blunted immunogenicity of SC inoculation as measured by peak titers and response rates. Further, the qualitative and quantitative deficits in B- and T-CMI responses to primary alum adjuvanted protein antigens persisted even in strictly healthy elderly populations with verified IM placement compared to younger populations.

Clinical trial registration

ClinicalTrials.gov, NCT04162223. Registered 14 November 2019. Retrospectively registered.
2-methylacetoacetyl-coenzyme A thiolase (beta-ketothiolase) deficiency: one disease - two pathways
Authors: Grünert Sarah C. , Sass Jörn Oliver
Published: April 2020

Background

2-methylacetoacetyl-coenzyme A thiolase deficiency (MATD; deficiency of mitochondrial acetoacetyl-coenzyme A thiolase T2/ “beta-ketothiolase”) is an autosomal recessive disorder of ketone body utilization and isoleucine degradation due to mutations inACAT1.

Methods

We performed a systematic literature search for all available clinical descriptions of patients with MATD. Two hundred forty-four patients were identified and included in this analysis. Clinical course and biochemical data are presented and discussed.

Results

For 89.6% of patients at least one acute metabolic decompensation was reported. Age at first symptoms ranged from 2 days to 8 years (median 12 months). More than 82% of patients presented in the first 2 years of life, while manifestation in the neonatal period was the exception (3.4%). 77.0% (157 of 204 patients) of patients showed normal psychomotor development without neurologic abnormalities.

Conclusion

This comprehensive data analysis provides a systematic overview on all cases with MATD identified in the literature. It demonstrates that MATD is a rather benign disorder with often favourable outcome, when compared with many other organic acidurias.
Development and validation of next generation sequencing based 35-gene hereditary cancer panel
Authors: Chan Wing , Lee Mianne , Yeo Zhen Xuan , Ying Dingge , Grimaldi Keith A. , Pickering Craig , Yang Michael M. S. , Sundaram Senthil K. , Tzang Lawrence C. H.
Published: April 2020

Background

Understanding the genetic basis of cancer risk is a major international endeavor. The emergence of next-generation sequencing (NGS) in late 2000’s has further accelerated the discovery of many cancer susceptibility genes. The use of targeted NGS-based multigene testing panels to provide comprehensive analysis of cancer susceptible genes has proven to be a viable option, with the accurate and robust detection of a wide range of clinically relevant variants in the targeted genes being crucial.

Methods

We have developed and validated a targeted NGS-based test for hereditary cancer risk assessment using Illumina’s NGS platform by analyzing the protein-coding regions of 35 hereditary cancer genes with a bioinformatics pipeline that utilizes standard practices in the field. This 35-gene hereditary cancer panel is designed to identify germline cancer-causing mutations for 8 different cancers: breast, ovarian, prostate, uterine, colorectal, pancreatic, stomach cancers and melanoma. The panel was validated using well-characterized DNA specimens [NIGMS Human Genetic Cell Repository], where DNA had been extracted using blood of individuals whose genetic variants had been previously characterized by the 1000 Genome Project and the Coriell Catalog.

Results

The 35-gene hereditary cancer panel shows high sensitivity (99.9%) and specificity (100%) across 4820 variants including single nucleotide variants (SNVs) and small insertions and deletions (indel; up to 25 bp). The reproducibility and repeatability are 99.8 and 100%, respectively.

Conclusions

The use of targeted NGS-based multigene testing panels to provide comprehensive analysis of cancer susceptible genes has been considered a viable option. In the present study, we developed and validated a 35-gene panel for testing 8 common cancers using next-generation sequencing (NGS). The performance of our hereditary cancer panel is assessed across a board range of variants in the 35 genes to support clinical use.
Influenza infection elicits an expansion of gut population of endogenous Bifidobacterium animalis which protects mice against infection
Authors: Zhang Qiang , Hu Jin , Feng Jia-Wu , Hu Xiao-Tong , Wang Ting , Gong Wen-Xiao , Huang Kun , Guo Yi-Xiong , Zou Zhong , Lin Xian , Zhou Run , Yuan Yu-Qi , Zhang An-Ding , Wei Hong , Cao Gang , Liu Chen , Chen Ling-Ling , Jin Mei-Lin
Published: April 2020

Background

Influenza is a severe respiratory illness that continually threatens global health. It has been widely known that gut microbiota modulates the host response to protect against influenza infection, but mechanistic details remain largely unknown. Here, we took advantage of the phenomenon of lethal dose 50 (LD50) and metagenomic sequencing analysis to identify specific anti-influenza gut microbes and analyze the underlying mechanism.

Results

Transferring fecal microbes from mice that survive virulent influenza H7N9 infection into antibiotic-treated mice confers resistance to infection. Some gut microbes exhibit differential features to lethal influenza infection depending on the infection outcome. Bifidobacterium pseudolongum and Bifidobacterium animalis levels are significantly elevated in surviving mice when compared to dead or mock-infected mice. Oral administration of B. animalis alone or the combination of both significantly reduces the severity of H7N9 infection in both antibiotic-treated and germ-free mice. Functional metagenomic analysis suggests that B. animalis mediates the anti-influenza effect via several specific metabolic molecules. In vivo tests confirm valine and coenzyme A produce an anti-influenza effect.

Conclusions

These findings show that the severity of influenza infection is closely related to the heterogeneous responses of the gut microbiota. We demonstrate the anti-influenza effect of B. animalis, and also find that the gut population of endogenous B. animalis can expand to enhance host influenza resistance when lethal influenza infection occurs, representing a novel interaction between host and gut microbiota. Further, our data suggest the potential utility of Bifidobacterium in the prevention and as a prognostic predictor of influenza.
The MiLES intervention targeting employers to promote successful return to work of employees with cancer: design of a pilot randomised controlled trial
Authors: Greidanus Michiel A. , de Boer Angela G. E. M. , de Rijk Angelique E. , Frings-Dresen Monique H. W. , Tamminga Sietske J.
Published: April 2020

Background

Employers express a need for support to facilitate the return to work (RTW) process of employees with cancer. We have developed the MiLES intervention, an online toolbox targeting employers during the RTW of employees with cancer. To evaluate the MiLES intervention, we propose the design of a pilot randomised controlled trial (RCT). The aim of this pilot is to determine whether a future RCT to study the effectiveness of this intervention on successful RTW of employees with cancer is feasible. Secondary aims are to obtain preliminary results on the effectiveness of the intervention and to determine the sample size needed in a future definitive RCT.

Methods

A pilot RCT with a 6-month follow-up will be conducted. Using medical specialists at Dutch hospitals, we aim to enrol 90 participants diagnosed with cancer (<2 years earlier) aged 18–63 years who are in paid employment with an employer and who are currently sick-listed or partly sick-listed for <1 year. Participants randomised to the intervention group will be asked to inform their employer about the online toolbox supporting employers during the RTW process of employees with cancer. Participants in the control group will receive ‘care as usual’ from their employer. All measures will be assessed at the level of the employee using questionnaires at baseline and after 3 and 6 months of follow-up. The feasibility of a future RCT will be determined using criteria concerning method-related uncertainties and acceptability of the study protocol. The primary effect measure will be successful RTW (that is, RTW perceived as being successful by the cancer survivor themselves). This effect measure will be used to perform the sample size calculation for a future definitive RCT.

Discussion

The design is proposed to determine the feasibility to study the effectiveness of the MiLES intervention targeting employers on the successful RTW of employees diagnosed with cancer. This pilot RCT can increase the probability of a successful future definitive RCT on the effectiveness of the intervention and potentially obviate the need to carry out an unfeasible and resource-intensive study.

Trial registration

Dutch Trial Register (NTR): NL6758, NTR7627. Registered on 30 October 2018.
Relapse after conservative surgery combined with triptorelin acetate versus conservative surgery only in women with focal adenomyosis: study protocol for a multicenter, prospective, randomized controlled trial
Authors: Wang Wenwen , Ma Xiangyi , Zhang Wei , Li Zhiying , Wang Yan , Yu Zhiying , Zhang Chunlian , Hong Li , Luo Ruoyu , Xing Hui , Wang Wuliang , Yue Qingfen , Wei Jia , Zhang Minli , Wang Shixuan
Published: April 2020

Background

The preservation of fertility and integrity of the reproductive organs has increasingly been of concern to most women with adenomyosis. Adenomyomectomy is conservative surgery that is now widely applied; however, relapse is a serious problem after the operation. Postoperative treatment, such as gonadotropin-releasing hormone agonist (GnRHa) has been suggested to result in reducing the rate of disease recurrence. However, there is still a lack of evidence from randomized clinical trials examining the efficacy of GnRHa in decreasing the postoperative recurrence rate.

Method/design

Relapse after conservative surgery combined with triptorelin acetate versus conservative surgery only in women with focal adenomyosis is a multicenter, prospective, randomized controlled trial. The primary outcome is relapse assessed using a visual analogue scale (VRS) and numeric rating scale (NRS), pictorial blood loss assessment chart (PBAC) score, and the size of the uterus and the lesion as measured by two/three-dimensional color doppler ultrasonography (2D/3D-CDUS) or magnetic resonance imaging (MRI). The secondary outcomes include quality of life, clinical pregnancy, ovarian reserve, adverse events, assessment by the Short Form (36) Health Survey and Female Sexual Function index, serum follicle-stimulating hormone, estradiol levels, and anti-Muellerian hormone and so on. All these indexes are measured at 3, 6, 12, 18, 24, 30, and 36 months after conservative surgery.

Discussion

The result of this large, multicenter randomized trial will provide evidence for one of the strategies of long-term management in focal adenomyosis after conservative operation.

Trial registration

Chinese Clinical Trial Registry: ChiCTR1800014340. Registered on 6 January 2018.
LSD1 inhibition yields functional insulin-producing cells from human embryonic stem cells
Authors: He Fei , Li Ning , Huang Hai-Bo , Wang Jing-Bo , Yang Xiao-Fei , Wang Hua-Dong , Huang Wei , Li Fu-Rong
Published: April 2020

Background

Human embryonic stem cells represent a potentially unlimited source of insulin-producing cells for diabetes therapy. While tremendous progress has been made in directed differentiation of human embryonic stem cells into IPCs in vitro, the mechanisms controlling its differentiation and function are not fully understood. Previous studies revealed that lysine-specific demethylase 1(LSD1) balanced the self-renewal and differentiation in human induced pluripotent stem cells and human embryonic stem cells. This study aims to explore the role of LSD1 in directed differentiation of human embryonic stem cells into insulin-producing cells.

Methods

Human embryonic stem cell line H9 was induced into insulin-producing cells by a four-step differentiation protocol. Lentivirus transfection was applied to knockdown LSD1 expression. Immunofluorescence assay and flow cytometry were utilized to check differentiation efficiency. Western blot was used to examine signaling pathway proteins and differentiation-associated proteins. Insulin/C-peptide release was assayed by ELISA. Statistical analysis between groups was carried out with one-way ANOVA tests or a student’s t test when appropriate.

Results

Inhibition or silencing LSD1 promotes the specification of pancreatic progenitors and finally the commitment of functional insulin-producing β cells; Moreover, inhibition or silencing LSD1 activated ERK signaling and upregulated pancreatic progenitor associated genes, accelerating pre-maturation of pancreatic progenitors, and conferred the NKX6.1+ population with better proliferation ability. IPCs with LSD1 inhibitor tranylcypromine treatment displayed enhanced insulin secretion in response to glucose stimulation.

Conclusions

We identify a novel role of LSD1 inhibition in promoting IPCs differentiation from hESCs, which would be emerged as potential intervention for generation of functional pancreatic β cells to cure diabetes.
Double-strand breaks in lymphocyte DNA of humans exposed to [18F]fluorodeoxyglucose and the static magnetic field in PET/MRI
Authors: Brix Gunnar , Günther Elisabeth , Rössler Ute , Endesfelder David , Kamp Alexandra , Beer Ambros , Eiber Matthias
Published: April 2020

Background

Given the increasing clinical use of PET/MRI, potential risks to patients from simultaneous exposure to ionising radiation and (electro)magnetic fields should be thoroughly investigated as a precaution. With this aim, the genotoxic potential of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) and a strong static magnetic field (SMF) were evaluated both in isolation and in combination using the γH2AX assay detecting double-strand breaks in lymphocyte DNA.

Methods

Thirty-two healthy young volunteers allocated to three study arms were exposed to [18F]FDG alone, to a 3-T SMF alone or to both combined over 60 min at a PET/CT or a PET/MRI system. Blood samples taken after in vivo exposure were incubated up to 60 min to extend the irradiation of blood by residual [18F]FDG within the samples and the time to monitor the γH2AX response. Absorbed doses to lymphocytes delivered in vivo and in vitro were estimated individually for each volunteer exposed to [18F]FDG. γH2AX foci were scored automatically by immunofluorescence microscopy.

Results

Absorbed doses to lymphocytes exposed over 60 to 120 min to [18F]FDG varied between 1.5 and 3.3 mGy. In this time interval, the radiotracer caused a significant median relative increase of 28% in the rate of lymphocytes with at least one γH2AX focus relative to the background rate (p = 0.01), but not the SMF alone (p = 0.47). Simultaneous application of both agents did not result in a significant synergistic or antagonistic outcome (p = 0.91).

Conclusion

There is no evidence of a synergism between [18F]FDG and the SMF that may be of relevance for risk assessment of PET/MRI.
A vehicle tracking algorithm combining detector and tracker
Authors: Yang Bo , Tang Mingyue , Chen Shaohui , Wang Gang , Tan Yan , Li Bijun
Published: April 2020

Real-time multichannel video analysis is significant for intelligent transportation. Considering that deep learning and correlation filter (CF) tracking are time-consuming, a vehicle tracking method for traffic scenes is presented based on a detection-based tracking (DBT) framework. To design the model of vehicle detection, the You Only Look Once (YOLO) model is used, and then, two constraints including object attribute information and intersection over union (IOU), are combined to modify the vehicle detection box. This approach improves vehicle detection precision. In the design of tracking model, a lightweight feature extraction network model for vehicle tracking is constructed. An inception module is used in this model to reduce the computational load and increase the adaptivity of the network scale. And a squeeze-and-excitation channel attention mechanism is adopted to enhance feature learning. Regarding the object tracking strategy, the method of combining a spatial constraint and filter template matching is adopted. The observation value and prediction value are matched and corrected to achieve stable tracking of the target. Based on the interference of occlusion in target tracking, the spatial position, moving direction, and historical feature correlation of the target are comprehensively employed to achieve continuous tracking of the target.
3D interpretation of helicopter-borne frequency-domain electromagnetic (HEM) data from Ramså Basin and adjacent areas at Andøya, Norway
Authors: Baranwal Vikas Chand , Brönner Marco , Rønning Jan-Steinar , Elvebakk Harald , Dalsegg Einar
Published: April 2020

The Ramså Basin is situated on the strandflat on the northeastern side of Andøya between Ramså and Skarstein. It is the only known Mesozoic sedimentary basin onshore Norway and therefore represents an easily accessible analogue to study the setting and development of the offshore Mesozoic basins along the Norwegian continental shelf. Basement rocks (100–10,000 Ωm) and sedimentary rocks (5–1000 Ωm) have distinct ranges of the electrical resistivity. Resistivity of the subsurface can be investigated by electric and electromagnetic (EM) methods to delineate the extent of the sedimentary basin. This paper presents the interpretation of helicopter-borne frequency-domain electromagnetic (HEM) data and their correlation with data obtained from borehole logging and two-dimensional (2D) electrical resistivity tomography (ERT). Resistivity data obtained from these methods show a good agreement. Spatially constrained inversion (SCI) of HEM presents a three-dimensional (3D) subsurface resistivity image of a larger area in comparison to what could have been drawn from few ERT lines and borehole logging. HEM interpretation together with other ERT and borehole resistivity data indicates the extent of Ramså Basin beyond the presently mapped boundary. This extension is also supported by interpretation of earlier collected gravity, magnetic and seismic data. The Ramså sedimentary basin shows a wide range of electrical resistivity between ca. 10 and 600 Ωm at different depths. Two highly conductive areas are newly identified by HEM data interpretation. In one of these areas, outcropping graphite and sulfide minerals were found by field observations.
Development and definition of a simplified scoring system in patients with multiple myeloma undergoing stem cells transplantation on standard computed tomography: myeloma spine and bone damage score (MSBDS)
Authors: Tagliafico Alberto Stefano , Belgioia Liliana , Bonsignore Alessandro , Signori Alessio , Formica Matteo , Rossi Federica , Piana Michele , Schenone Daniela , Dominietto Alida
Published: April 2020

Background

In clinical practice, there is the need to optimize imaging usage in MM patients. Accordingly, the aim of this paper was to develop a simple computed tomography (CT) scoring method for MM, able to shorten and simplify the interpretation time with good intra- and inter-reader reliability. This method, named MSBDS (Myeloma Spine and Bone Damage Score) was developed with the final aim to use standard total-body CT in the routine practice of MM centres as a complement of standard evaluations in patients undergoing stem cells transplantation.

Methods

We used a widely accepted consensus formation method and literature research during three structured face-to-face meetings specifically designed to combine opinions from a group of experts with proven experience in multiple myeloma care and/or musculoskeletal CT to facilitate the consensus on the field of study topics and the contents of the MSBDS score. Seven practical requisites for the MSBDS score were agreed. A total of 70 MM patients (mean age, 60 years ±9.2 [standard deviation]; range, 35–70 years) undergoing total-body CT was included to develop MSBDS scores. Patients data were already stored in the Radiological database for other Research studies IRB approved (054/2019). Readers to test the MSDMS were radiologists and clinicians involved in MM care or expert in bone damage scores with different level of experience in musculoskeletal and total body CT. Readers were blinded to the clinical data of the patients.

Results

The MSBDS scores based on the consensus work described above and literature analysis was finalized. MSBDS is based on an additive scale with assessment of a total body CT with the bone window one time and includes indicators of structural bone damage and instability or fracture risk. The total score is given by the sum of item scores for abnormalities detected. Its values range from 0 (minimum) to values > 10 where 10 is represented by high-risk patients. In high-risk patients immediate surgical or radiation oncologist consultation is suggested.

Conclusions

The MSBDS descriptive criteria are easy, highly reproducible and can be considered as a strong base for harmonizing total body CT interpretation in multiple myeloma patients undergoing stem cell transplantation.
Effect of Non-Steroidal Anti-Inflammatory Drugs on Sport Performance Indices in Healthy People: a Meta-Analysis of Randomized Controlled Trials
Authors: Cornu Catherine , Grange Clémence , Regalin Amanda , Munier Justine , Ounissi Sonia , Reynaud Natane , Kassai-Koupai Behrouz , Sallet Pierre , Nony Patrice
Published: April 2020

Background

Non-steroidal anti-inflammatory drugs (NSAIDs) are medications that are frequently used by athletes. There may also be some abuse of these substances, although it is unclear whether NSAIDs in fact enhance performance.

We performed a systematic review and meta-analysis to evaluate the effect of NSAIDs on sport performance indices.

Methods

We selected randomized trials from the PubMed and Cochrane Library databases investigating the effects of NSAIDs on sport performance. Volunteers could be healthy adult men and women. Any NSAID, administered by any route, taken prior to any type of exercise, and for any duration could be used. The control intervention could be a placebo, an active substance, or no intervention. We included double-blind, single-blind, and open-label studies. The primary outcome was the maximum performance in exercises as defined in each study. The secondary outcomes were the time until self-reported exhaustion and the self-reported pain.

Results

Among 1631 records, we retained thirteen parallel-group and ten crossover studies, totaling 366 and 148 subjects, respectively. They were disparate regarding treatments, dose and duration, and the type of exercise. There was neither significant difference in the maximum performance between NSAIDs and control groups nor in the time until exhaustion nor in self-perceived pain.

Conclusions

The existence of an ergogenic effect of NSAIDs on sport performance indices was unable to be concluded, since the level of evidence of the studies is low, the doses tested, and the exercises performed are very heterogeneous and far from those observed in real-life practices. More studies are required.
Nematicidal activity of aqueous and organic extracts of local plants against Meloidogyne incognita (Kofoid and White) Chitwood in Algeria under laboratory and greenhouse conditions
Authors: Zaidat Sabri Ala Eddine , Mouhouche Fazia , Babaali Djaafar , Abdessemed Nesma , De Cara Miguel , Hammache Miloude
Published: April 2020

The nematicidal efficiency of two types of extracts (aqueous and methanolic) of 5 plants Peganum harmala L., Raphanus raphanistrum L., Taxus baccata L., Sinapis arvensis L., and Ricinus communis L. on second stage juveniles (J2s) of Meloidogyne incognita was evaluated at 4 doses in vitro (40, 50, 60 and 80%) at 4 exposure times (12, 16, 24, and 32 h). In a greenhouse, methanol extracts obtained from 0.75, 1.5, or 3 g of the dry matter of T. baccata, S. arvensis, and P. harmala were added in pots of tomato plants (Moneymaker cultivar) to test the effect of the extracts on the nematode, its reproduction, and the plant’s growth. After 32 h of exposure, the in vitro results on J2s showed that all the aqueous and methanolic extracts of the 5 plants had positive effects on J2 mortality compared to controls; water and DMSO (2%) (dimethyl sulfoxyde). In general, methanolic extracts were significantly more effective than the aqueous ones. Methanolic extracts of T. baccata, P. harmala, and S. arvensis had the highest mortality rates (100, 89.2, 86.6%), respectively, followed by the aqueous extract of T. baccata (73.8%). After 12 days, the hatching inhibition varies between (61.4%) for the aqueous extract of R. communis and (84.2%) for the methanolic extract of T. baccata. The average was significantly different from controls (DMSO 23.8 and water 21.8%) for all extracts. Methanolic extracts were not always significantly different than the aqueous ones. In vivo methanolic extracts of S. arvensis, P. harmala, and T. baccata reduced infestation than the controls. The gall index varied between 3.5 and 5.5 for the extracts than the positive control 6.5. The present study revealed the effectiveness of all tested plant extracts to root-knot nematodes without any chemical inputs.
Feasibility, Acceptability, and Efficacy of a Community Health Worker–Driven Approach to Screen Hard-to-Reach Periurban Women Using Self-Sampled HPV Detection Test in India
Authors: Poli Usha Rani , Muwonge Richard , Bhoopal Triveni , Lucas Eric , Basu Partha
Published: April 2020

PURPOSE

Detection of high-risk human papillomavirus (HPV) in self-collected vaginal samples can significantly improve participation of hard-to-reach women; however, the implementation of such an approach in a real-life setting, especially in countries with limited resources, has certain challenges. Our study aimed to evaluate the feasibility, acceptability, and efficacy of implementing an HPV self-sampling–based approach to screen a socioeconomically disadvantaged, unscreened population with support from community health workers (CHWs) for community mobilization, counseling, and navigation. Different triaging options for HPV-positive women were also assessed.

METHODS

Women age 30 to 65 years from low socioeconomic periurban areas who had never been screened before were motivated by CHWs to attend local community centers and provide self-collected vaginal samples for careHPV testing. Screen-positive women were informed and navigated by CHWs to attend colposcopy clinics where cervical biopsy and same-day ablative treatment were offered.

RESULTS

Women readily accepted to provide self-collected samples after counseling by CHWs. Screen positivity was 6.4%, and CHWs successfully navigated 65% of HPV-positive women to colposcopy. Cervical intraepithelial neoplasia (CIN) 2+ detection rate was 9.7 per 1,000 women screened. The HPV test had a positive predictive value of 15.3% to detect CIN 2+ lesions. Triage using visual inspection with acetic acid significantly improved the positive predictive value (49.5% to detect CIN 2+), but missed a significant number of CIN 2+ lesions. Colposcopy sensitivity was also suboptimal. Of 51 women with lesions, 80% underwent ablative treatment and the majority accepted same-day treatment.

CONCLUSION

CHW-driven self-sampling for HPV testing is feasible, well-accepted, and effective for screening unscreened hard-to-reach women. The screen-and-treat approach can ensure strong linkage between screening and treatment.
Harmonious Insurrections
Authors: Nichols Shaun S.
Published: April 2020

Exceptionalism has long defined our understanding of the rise of progressive politics in the early twentieth-century United States. While industrialized European nations blazed the path of social democracy, in the United States, it is argued, “progressivism” merely legitimized middle-class cultural hegemony, social engineering, and the subversion of working-class power. In this era, however, social reform was a distinctly state-led phenomenon, only rarely taken up by the federal Congress. As such, by analyzing labor protest at the level of the state—in this case, Washington—a different interpretation emerges. American “progressivism” was neither exceptionally repressive nor of little interest to labor. In fact, espousing a language of progress, the common good, and the duty of the state to promote “social harmony,” Washington workers actively drew on “progressive” ideas in their struggles to tame the excesses of industrial capitalism. This ideology of “labor progressivism” became the foundation for unprecedented working-class power.
PRP8A and PRP8B spliceosome subunits act coordinately to control pollen tube attraction in Arabidopsis thaliana
Authors: Kulichová Katarína , Kumar Vinod , Steinbachová Lenka , Klodová Božena , Timofejeva Ljudmilla , Juříček Miloslav , Honys David , Hafidh Said S.
Published: April 2020

Precise guided pollen tube growth by the female gametophyte is a prerequisite for successful sexual reproduction in flowering plants. Cysteine-rich proteins (CRPs) secreted from the embryo sac are known pollen tube attractants perceived by pollen tube receptor-like kinases. How pre-mRNA splicing facilitates this cell-to-cell communication is not understood. Here, we report a novel function of Pre-mRNA PROCESSING factor 8 paralogs, PRP8A and PRP8B, as regulators of pollen tube attraction. Double mutant prp8a prp8b ovules cannot attract pollen tubes, and prp8a prp8b pollen tubes fail to sense the ovule's attraction signals. Only 3% of ovule-expressed genes were misregulated in prp8a prp8b. Combination of RNA sequencing and the MYB98/LURE1.2-YFP reporter revealed that the expression of MYB98, LUREs and 49 other CRPs were downregulated, suggesting loss of synergid cell fate. Differential exon usage and intron retention analysis revealed autoregulation of PPR8A/PRP8B splicing. In vivo, PRP8A co-immunoprecipitates with splicing enhancer AtSF3A1, suggesting involvement of PRP8A in 3′-splice site selection. Our data hint that the PRP8A/PRP8B module exhibits spliceosome autoregulation to facilitate pollen tube attraction via transcriptional regulation of MYB98, CRPs and LURE pollen tube attractants.
Interest Rates under Falling Stars
Authors: Bauer Michael D. , Rudebusch Glenn D.
Published: April 2020

Macro-finance theory implies that trend inflation and the equilibrium real interest rate are fundamental determinants of the yield curve. However, empirical models of the term structure of interest rates generally assume that these fundamentals are constant. We show that accounting for time variation in these underlying long-run trends is crucial for understanding the dynamics of Treasury yields and predicting excess bond returns. We introduce a new arbitrage-free model that captures the key role that long-run trends play in determining interest rates. The model also provides new, more plausible estimates of the term premium and accurate out-of-sample yield forecasts. (JEL E31, E43, E47)
Decontamination methods of personal protective equipment for repeated utilization in medical/surgical settings
Authors: Kharbat Abdurrahman , Mizer Adin , Zumwalt Mimi
Published: April 2020

The COVID-19 pandemic has affected citizens and healthcare workers worldwide due to a number of important factors. The transmission of the SARS CoV-2 microorganism, the pathogen that causes COVID-19 infection, occurs through droplet and aerosol spread due to coughs and sneezes from infected patients. A panicked public began hoarding medical supplies and personal protective equipment (PPE), leaving healthcare workers to care for patients without adequate protection. A literature review was conducted to better understand the options available to hospital and healthcare system administrators as they develop necessary protocols for the conservation and possible reuse of PPE. This review is based upon the peer-reviewed studies of various scientific investigators, biotechnology researchers, governmental agency health officials, including meta-analyses, preliminary/pilot studies, and policy statements. Current findings indicate that extended usage of N95 respirators is practical since there are methods available for the decontamination/repeated use of N95 respirators. In evaluating the efficacy of such methods, the safety of healthcare workers is important in deciding which method to recommend. Available evidence supports the use of the Bioquell Hydrogen Peroxide Vapor (HPV) system for decontaminating N95 respirators. Information on other PPE will also be discussed about more specific items. Informed decisions regarding the policies of hospitals and healthcare systems must be considered, and with the safety of healthcare workers in mind, both factors influenced the recommendations made in this comprehensive review.
The Ambiguity of Earnings Announcements
Authors: Budanova Sofya , Cianciaruso Davide , Marinovic Iván
Published: April 2020

We study the consequences of misreporting in settings where ambiguity-averse investors face uncertainty about two aspects of the firm: its productivity and the weakness of its reporting system. We show that the joint presence of these two sources of uncertainty distorts the firm’s investment choices in opposing ways, leading to overinvestment by large firms (to signal productivity) and underinvestment by small firms (to signal that the reporting system is not weak). Our analysis suggests that uncertainty regarding reporting weakness affects both the level of the market-to-book ratio and its association with firm size. In addition, we show that, under plausible circumstances, reductions in uncertainty can be detrimental to social welfare: lower information asymmetry about reporting weakness always encourages more aggressive misreporting and boosts investment, thereby exacerbating the possible overinvestment problem faced by some firms.

This paper was accepted by Suraj Srinivasan, accounting.
An Approximation Algorithm for Network Revenue Management Under Nonstationary Arrivals
Authors: Ma Yuhang , Rusmevichientong Paat , Sumida Mika , Topaloglu Huseyin
Published: April 2020

Many revenue management problems require making capacity control and pricing decisions for multiple products. The decisions for the different products interact because either the products use a common pool of resources or the customers choose and substitute among the products. When pricing airline tickets, for example, different itinerary products use the capacities on common flight legs and the customers choose and substitute among different itinerary products that serve the same origin-destination pair. Finding the optimal capacity control and pricing decisions in such problems can be challenging because one needs to simultaneously consider the capacities available to serve a large pool of products. In “An Approximation Algorithm for Network Revenue Management under Nonstationary Arrivals,” Ma, Rusmevichientong, Sumida, and Topaloglu develop efficient methods to make decisions with performance guarantees in high-dimensional capacity control and pricing problems.
Feeling/being ‘out of place’: psychic defence against the hostile environment
Authors: Fang Nini
Published: April 2020

What is it like to be an immigrant worker in a ‘hostile environment’ in the UK? How does the form of discursive environment, which sees immigration as a social epidemic, impact on an immigrant worker’s experience of their cultural (dis)localities and subjectivity? In this article, I draw on my personal, psychoanalytically informed voice to explore these questions, by foregrounding the materiality of the hosting environment as the place in which the present relational matrix takes place, in which the internal dynamics of object relations are lived in the present sense, and the idiosyncratic expression of selfhood assumes forms.

The materialised reality of the place matters not least because it is drenched in power relations but also as it is where an immigrant worker seeks to live. The hostile host, in this sense, sees immigrants not simply as its guests (Derrida and Dufourmantelle, 2000), but as unwelcome yet persistent guests to be yoked to their place of otherness and inferiority. By presenting vignettes of my encounters with the Home Office, I call into question the existential conditions of the immigrant worker and the potentiality for object-relatedness on relational grounds problematically punctured by hostile rhetoric. Could an immigrant’s sense of locality ever be anything but – evoking Said ([1999] 2013) – ‘out of place’? To address this, I will explore ‘out of place’ not simply as an emotional, lived experience, but also as a state of being that is embodied, psychically worked on and strategically evoked in resisting the power of the hostile host.
Multiple shots averaging in laser flash measurement
Authors: Ferrarini Giovanni , Bortolin Alessandro , Cadelano Gianluca , Finesso Lorenzo , Bison Paolo
Published: April 2020

The laser flash method is a well-known procedure to determine the thermal diffusivity of a wide range of materials. However, in some cases there is the need of limiting the input power, measuring materials with high thermal capacity, or investigating thick samples. These conditions lead to a reduction of the signal-to-noise ratio. Therefore, we propose a new laser flash control and data acquisition system, that is able to repeat multiple times the emission of the laser impulse and the measurement of the thermal response of the specimen. With the average of several measurements, it is possible to obtain a decrease of the noise when working with low power inputs.
Defect mode tunability based on the electro-optical characteristics of the one-dimensional graphene photonic crystals
Authors: Aly Arafa H. , Sayed Fatma A. , Elsayed Hussein A.
Published: April 2020

New (to the best of our knowledge) photonic crystal optical filters with unique optical characteristics are theoretically introduced in this research. Here, our design is composed of a defect layer inside one-dimensional photonic crystals. The main idea of our study is dependent on the tunability of the permittivity of graphene by means of the electro-optical effect. The transfer matrix method and the electro-optical effect represent the cornerstone of our methodology to investigate the numerical results of this design. The numerical results are investigated for four different configurations of the defective one-dimensional photonic crystals for the electric polarization mode. The graphene as a defect layer is deposited on two different electro-optical materials (lithium niobate and polystyrene) to obtain the four different configurations. The electro-optical properties of graphene represent the main role of our numerical results. In the infrared wavelength range from 0.7 µm to 1.6 µm, the reflectance properties of the composite structures are numerically simulated by varying several parameters such as defect layer thickness, applied electrical field, and incident angle. The numerical results show that graphene could enhance the reflectance characteristics of the defect mode in comparison with the two electro-optical materials without graphene. In the presence of graphene with lithium niobate, the intensity of the defect mode increased by 5% beside the shift in its position with 41 nm. For the case of polystyrene, the intensity of the defect mode increased from 6.5% to 68.8%, and its position is shifted with 72 nm. Such a design could be of significant interest in the sensing and measuring of electric fields, as well as for filtering purposes.