bluuebunny/crossref_metadata_2025 huggingface dataset:
Cuidados e acolhimento na atenção primária à saúde de mulheres vítimas de violência: uma visão da epidemiologia e da literatura
Authors: Silva Jean Rodrigo Neves Farias Portela da , Souza Pedro Henrique Bacelar Martins Pereira Pinto de , Magalhães Lucas Zanforlin , Guimarães Murilo Ian do Vale , Freitas Vanessa de Oliveira , Mocbel Yan Matheus Arnaud , Ramos Thiago José Maués , Moraes Naiana Palheta , Dias Petrus Calixtro Guilhermino Pereira de Sousa , Santos Gabriel Franco de Carvalho dos
Published: June 2022

A violência imposta à mulher acontece frequentemente no meio intrafamiliar e as principais formas praticadas são a violência física, violência psicológica, violência sexual, violência patrimonial e violência moral. Assim, frente a escassa abordagem desta temática em cursos de graduação, o profissional de saúde, com destaque para o médico, enfrenta dificuldades para atuar em situações de violência. Desse modo, o objetivo desse trabalho é discutir a importância da comunicação no setor saúde para o acolhimento de mulheres vítimas de violência no Brasil. Os resultados mostraram que o perfil epidemiológico da violência cometida contra mulheres no período que vai de 2009 a 2021, o Estado do Pará notificou ao SINAN um total de 55. 490 casos de violência doméstica, sexual e/ou outras violências contra mulheres. Em média, foram notificados aproximadamente 3.527 casos por ano, sendo 2020 e 2009 os anos que apresentaram, respectivamente, a maior e a menor contagem de casos: 8.640 e 212. A literatura acerca da violência contra a mulher no cenário brasileiro mostra que este é um problema multifacetado, onde existem falhas na notificação, aliado a falhas no sistema de segurança pública que são intensificadas por fatores sociais. Portanto, vale ressaltar a importância da ampliação das práticas de comunicação entre os profissionais de saúde para a realização do acolhimento eficiente das mulheres vítimas de violência doméstica, com o foco no atendimento integral e humanizado.
Método não invasivo versus método invasivo para medição de pressão arterial em roedores: uma revisão integrativa da literatura
Authors: Valério Mariana da Palma , Martini Silvia Cristina , Boschi Silvia Regina Matos da Silva , Scardovelli Terigi Augusto , Silva Alessandro Pereira da
Published: June 2022

A aferição da pressão arterial (PA) é o principal parâmetro de monitoramento relacionado ao sistema cardíaco, e o uso de animais é vital para a compreensão do mecanismo fisiológico e estudo de patologias relacionadas a esse sistema. Os procedimentos de aferição em roedores mais recomendados pela literatura são: O método não invasivo (punho de cauda) e os métodos invasivos (cateter com fluido e telemetria). O objetivo deste estudo foi verificar as principais técnicas de medição arterial em roedores, avaliar seus pontos positivos, negativos e classificar qual a técnica mais viável e eficaz para ensaios experimentais iniciais. Foi realizado uma revisão integrativa da literatura utilizando os descritores e seus respectivos sinônimos, “real time device”, “blood pressure measurements”, “rats” fazendo o uso do booleano “AND” nas bases de dados PubMed, Scielo e Medline. Como critérios de inclusão foram adotados artigos que abordassem a temática supracitada, em português, inglês ou espanhol, completos, de ensaios experimentais em roedores, publicados com no máximo nos últimos 21 anos. Os critérios de exclusão foram artigos não disponíveis para leitura na integra e estudos que não utilizassem roedores como amostra. Foram selecionados 13 artigos que atendiam aos critérios. Os resultados demonstram que os métodos invasivos podem ser vantajosos para medições contínuas, mas que preferencialmente seria recomendado utilizar o método não invasivo por ser mais seguro e de fácil execução. Conclui-se que para ensaios experimentais iniciais, o procedimento a ser escolhido seria o método não invasivo, punho de cauda.
FORMAÇÃO E ATUAÇÃO PROFISSIONAL DE INTÉRPRETES EDUCACIONAIS DE LIBRAS EM AULAS DE MATEMÁTICA
Authors: Maria da Fonseca Pinto Gisela , Coelho de Segadas-Vianna Claudia
Published: June 2022

"A presença de alunos surdos nas salas de aula da educação básica vem colocando em destaque um profissional que era percebido apenas em grandes eventos, denominado, neste caso, de tradutor-intérprete de Libras. A atuação em uma sala de aula, especificamente em aulas de matemática, implica em situações que diferem bastante daquelas até então vivenciadas por esses profissionais. Nesse texto, são apresentados resultados de uma pesquisa conduzida com duas intérpretes educacionais de Libras, com objetivo de analisar as interações em sala de aula entre alunos surdos,intérpretes e professores em aulas de matemática, com foco específico nas ações mediadoras dos intérpretes e em seus percursos formativos Inspirando-se na metodologia de auto confrontação, as aulas foram filmadas, além de terem sido conduzidas entrevistas semiestruturadas com os intérpretes, para posterior categorização dos dados. Os resultados indicam a necessidade de que se repense a formação do intérprete e a definição de suas funções, que são distintas das do professor."
UMA CONVERSA SOBRE INSUBORDINAÇÃO CRIATIVA COM CELI LOPES: COMO NÃO LEMBRAR DAQUELES COLEGAS RESPONSAVELMENTE SUBVERSIVOS E OUSADOS! COMO NÃO LEMBRAR
Authors: Gonçalves Pita Ana Paula , Coelho Lima Priscila , Roncato Célia Regina
Published: June 2022

"Objetiva-se, com esse artigo, entender como os conceitos de Insubordinação Criativa e Subversão Responsável foram elaborados a partir de práticas docentes e formação de professores. Especificamente, buscamos compreender de que maneira tais conceitos são utilizados na educação e como ações pedagógicas criativamente insubordinadas reverberam positivamente nos processos de ensino e de aprendizagem. Os dados apresentados foram decorrentes da realização de uma entrevista com a Professora Celi Lopes, momento em que buscamos refletir sobre algumas relações entre os conceitos discutidos e a formação de professores, além de produções científicas da referida Professora com Beatriz D’Ambrosio. Os resultados trazem indícios da necessidade de espaços para a escuta e o diálogo com professores e gestores, na intenção de práticas voltadas ao bem dos e das estudantes."
AYDA IGNEZ ARRUDA: ASPECTOS HISTÓRICOS DE SUA TRAJETÓRIA CIENTÍFICA NO PARANÁ, ANOS 1956-1968
Authors: Rita Andrade Machado Suélen , M. Trivizoli Lucieli
Published: June 2022

"No presente artigo, apresentamos a matemática catarinense Ayda Ignez Arruda e aspectos históricos de sua trajetória científica no Paraná, anos 1956 a 1968. Para tanto, situamos que os resultados aqui apresentados correspondem a fragmentos de uma pesquisa de doutorado que foi desenvolvida no âmbito do Programa de Pós-Graduação em Educação para a Ciência e a Matemática, da Universidade Estadual de Maringá. A pesquisa teve como objetivo a composição de um estudo histórico-biográfico de Ayda Ignez Arruda e suas contribuições para a institucionalização da Lógica Matemática no Brasil. Para a escrita deste artigo, utilizamos a abordagem qualitativa e os pressupostos da escrita biográfica, que permitem flexibilidade na construção de uma narrativa que conduz vestígios históricos documentais e bibliográficos sobre a personagem. Baseadas nessa interface, verificamos que Ayda Ignez Arruda, no final da década de 1950, bacharelou-se e se licenciou em Matemática pela Universidade Católica do Paraná. Na década de 1960, doutorou-se em Matemática na área de Lógica Matemática pela Universidade Federal do Paraná, sob supervisão de Newton Carneiro Affonso da Costa, Livre Docência da Cadeira de Análise Matemática da Universidade Federal do Paraná. Foi sócia da Sociedade Paranaense de Matemática e junto à Da Costa contribuiu com a Escola de Curitiba, cujas vertentes de estudo se alinhavam aos estudos da Lógica Matemática."
FORMAÇÃO INICIAL E CONTINUADA DO PROFESSOR QUE ENSINA MATEMÁTICA NA PERSPECTIVA INCLUSIVA: UM MAPEAMENTO SISTEMÁTICO DA LITERATURA ACADÊMICA
Authors: Brito da Silva Rosimeire , Mendes Sant’Ana Izabella
Published: June 2022

"Considerando o histórico da Formação Matemática docente em nosso país e o papel do professor que ensina matemática, o objetivo deste estudo foi identificar pesquisas que contemplem a formação do professor que ensina matemática na perspectiva da inclusão de alunos público-alvo da Educação Especial. Neste sentido, realizou-se um mapeamento sistemático de teses, dissertações e artigos inseridos no Catálogo da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, na Biblioteca Digital Brasileira de Teses e Dissertações e no Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, publicados entre os anos 2000 e 2019. A escolha das bases se deu pelo grau de abrangência e concentração de trabalhos; o recorte temporal justificou-se pelas políticas de inclusão implementadas nesse período. Em geral, os dados revelam que existe uma tendência na realização de pesquisas centradas na formação inicial, com ênfase na análise do currículo/projeto de formação em inclusão, demonstrando que nem todas as universidades apresentam na grade curricular, em caráter obrigatório, disciplinas que contemplem a Educação Matemática Inclusiva; as poucas ofertadas configuram-se em modo experimental ou optativo. Concernente à formação continuada, discute-se a necessidade da criação de mais cursos voltados à formação matemática inclusiva, em caráter teórico-prático e em nível de pós-graduação."
PENSAMENTO CRÍTICO E CRIATIVO EM MATEMÁTICA A PARTIR DA APRENDIZAGEM DIALÓGICA E INVESTIGATIVA
Authors: Gianni Fonseca Mateus , Marcondes de Oliveira Pires Leandro , Carneiro Dörr Raquel , Delaine Teixeira Zanetti Matheus , Hércules Gontijo Cleyton
Published: June 2022

"O conhecimento matemático da população tem sido questionado ano após ano, seja em decorrência dos resultados de avaliações nacionais, seja de avaliações internacionais. Além disso, atualmente seguem novas propostas de capacidades a serem desenvolvidas junto à matemática escolar, tão logo torna-se cada vez mais evidente a necessidade do desenvolvimento do pensamento crítico e criativo nessa área de saber. O objetivo desta experiência foi analisar se a prática dialógica e investigativa contribui para o aprimoramento das aprendizagens dos conteúdos matemáticos e do pensamento crítico e criativo nessa área junto a estudantes do sétimo ano do Ensino Fundamental. Para tanto, fez-se uso de metodologia qualitativa e exploratória, de modo que os estudantes recebiam feedbacks semanais, com o objetivo de estimulá-los a produzirem respostas escritas. Por resultados, as elaborações por parte dos estudantes quanto aos conteúdos matemáticos, forneceram pistas que o ato do diálogo contribuiu para que pudessem criar suas ideias, refletir, criticar, enfim, elaborar matemática de forma crítica e criativa."
MODELING AND PROJECTS: HEALTHY EATING, ENVIRONMENTAL AWARENESS AND PROPOSING A THEME
Authors: Galleguillos Jeannette , Patricio Valdivia José , Guajardo Pablo
Published: June 2022

"This work presents a proposal of modeling activities designed and implemented in a seventh-level course (12 years old). The activities include three phases: understanding modeling, modeling itself, and formulating modeling projects. These phases are intended to facilitate the modeling process. The activities are contextualized in themes of interest: healthy eating, awareness of the garbage produced, and the third phase, student proposals of a theme and problem. These proposals emphasize the development of contextualized problems where the mathematics emerge naturally from the themes. The aim of the study was to understand the difficulties and potentialities of implementing the modeling activities and was approached through a qualitative research, collecting data from the students' productions, and an interview with the course teacher. Grounded theory was used to analyze the data, from which categories emerged relating to students’ and teachers’ difficulties and potentialities. Results indicate that, on one hand, the proposal allowed students to visualize the mathematical difficulties and thus, support them; on the other hand, working with contextualized situations allowed the students to participate in the activities and develop communication and complementary skills. These activities allowed students to relate mathematics to real-life situations, visualizing its importance and identifying how it can be used in diverse contexts."
TRINCAS PITAGÓRICAS E NÚMEROS FIGURADOS: UM ENFOQUE HISTÓRICO PARA O ENSINO DO TEOREMA DE PITÁGORAS
Authors: Mendes Duarte Augusto , Seidi Osiro Yamamoto Fábio
Published: June 2022

"Objetivando articular História da Matemática, Resolução de problemas, uso de materiais manipuláveis e de recursos digitais, foi construída uma proposta didática em torno da origem aritmética do Teorema de Pitágoras. O projeto ocorreu em quatro turmas do nono ano do Ensino Fundamental de uma escola particular de São Paulo, durante quatro aulas ministradas ao longo de uma semana. Cada turma continha em torno de 20 alunos com aproximadamente 14 anos. Por meio de uma exposição dialogada sobre o contexto histórico em que se encontravam os pitagóricos, os discentes experienciaram um processo de investigação análogo ao da doutrina pitagórica. Dispostos em grupos e utilizando fichas coloridas, investigaram os números figurados e resolveram problemas direcionadores. As conclusões foram discutidas em plenária e os novos conceitos foram formalizados e contextualizados historicamente. Para facilitar a visualização de situações históricas e conceitos recém descobertos, animações previamente desenvolvidas pelos professores foram utilizadas. Considera-se que a articulação dos recursos utilizados favoreceu o aspecto motivacional da aprendizagem bem como a formação de conceitos e de procedimentos relativos ao Teorema de Pitágoras. Tais resultados positivos foram evidenciados tanto pelas resoluções e interações em plenária quanto através das soluções apresentadas por escrito."
UMA ENTREVISTA COM JOÃO PEDRO DA PONTE SOBRE A INVESTIGAÇÃO MATEMÁTICA NA EDUCAÇÃO MATEMÁTICA
Authors: Wichnoski Paulo
Published: June 2022

Entrevista
PRODUZINDO INFINITOS: UM ESTUDO SOB O OLHAR DO MODELO DOS CAMPOS SEMÂNTICOS
Authors: Aparecido Salatta Vinícius , Carrazedo Dantas Sérgio
Published: June 2022

"Apresentamos nesse trabalho um recorte de uma pesquisa de mestrado realizada pelos autores sobre um estudo das produções de significado para infinito feita por alunos de Cursos de Graduação em Matemática com o auxílio do software GeoGebra. A produção e análise dos dados fundamentam-se no Modelo dos Campos Semânticos de Lins (2012), de modo que, ao nos desprender de uma definição pronta e acabada para infinito, ficamos abertos a novas interpretações e tornamos plausíveis certas direções de interlocução manifestadas pelos alunos, porém, sem atributos de certo ou errado. Para a produção dos dados, realizamos um questionário online pela plataforma do GeoGebra utilizando dois recursos: o GeoGebra Materiais e o GeoGebra Classroom, os quais além de possuírem recursos semelhantes a outros questionários como o Google Forms, por exemplo, ainda permitem acrescentar construções interativas do GeoGebra. Outro motivo pela opção do questionário online decorre do cenário pandêmico causado pela doença COVID-19, ao passo que os sujeitos foram convidados por e-mail e grupos de redes sociais e sua aplicação se deu por meio do envio do link do questionário por e-mail aos interessados. Após a produção e análise dos dados, percebemos que os sujeitos de pesquisa manifestam crenças-afirmações cujas legitimidades são advindas tanto de suas experiências do dia a dia quanto de suas experiências na cultura acadêmica de um Curso de Graduação em Matemática. Também destacamos que suas enunciações apresentam justificações pelo zoom e o próprio enunciado das questões."
Experimental Study of Wave Attenuation Across an Artificial Salt Marsh
Authors: Baker Scott , Murphy Enda , Cornett Andrew , Knox Paul
Published: June 2022

Scaled laboratory experiments were conducted to investigate the effectiveness of marsh vegetation in dissipating wave energy and reducing wave overtopping discharges at the crest of a dyke located immediately landward of the marsh. Model dyke and marsh platform features, loosely based on archetypes found in Atlantic Canada, were constructed in a wave basin at 1:20 scale and exposed to a broad range of waves and water level conditions. The 2D experiments were conducted using idealized surrogate vegetation (both rigid and flexible), and the model setup featured four parallel flumes which enabled four alternative configurations to be investigated simultaneously. The experiments investigated the sensitivity of wave attenuation and overtopping to the length of the vegetation field, vegetation characteristics (stem density, height, and flexibility) and varying water levels and wave conditions. The study outputs have helped to address knowledge gaps and provide evidence to support and inform broader use of hybrid marsh-dyke systems and managed dyke realignment to help manage flood and erosion risk and improve coastal resilience in Canada and internationally. This research confirmed the benefit of tidal flats hosting coastal marshes for attenuating waves, reducing overtopping volumes and lessening damage to dyke structures. As expected, taller and denser marshes were more effective in attenuating wave energy for a given marsh width.
Inhibition of the Protein Arginine Methyltransferase PRMT5 in High-Risk Multiple Myeloma as a Novel Treatment Approach
Authors: Vlummens Philip , Verhulst Stefaan , De Veirman Kim , Maes Anke , Menu Eline , Moreaux Jérome , De Boussac Hugues , Robert Nicolas , De Bruyne Elke , Hose Dirk , Offner Fritz , Vanderkerken Karin , Maes Ken
Published: June 2022

Multiple myeloma (MM) is an incurable clonal plasma cell malignancy. Subsets of patients have high-risk features linked with dismal outcome. Therefore, the need for effective therapeutic options remains high. Here, we used bio-informatic tools to identify novel targets involved in DNA repair and epigenetics and which are associated with high-risk myeloma. The prognostic significance of the target genes was analyzed using publicly available gene expression data of MM patients (TT2/3 and HM cohorts). Hence, protein arginine methyltransferase 5 (PRMT5) was identified as a promising target. Druggability was assessed in OPM2, JJN3, AMO1 and XG7 human myeloma cell lines using the PRMT5-inhibitor EPZ015938. EPZ015938 strongly reduced the total symmetric-dimethyl arginine levels in all cell lines and lead to decreased cellular growth, supported by cell line dependent changes in cell cycle distribution. At later time points, apoptosis occurred, as evidenced by increased AnnexinV-positivity and cleavage of PARP and caspases. Transcriptome analysis revealed a role for PRMT5 in regulating alternative splicing, nonsense-mediated decay, DNA repair and PI3K/mTOR-signaling, irrespective of the cell line type. PRMT5 inhibition reduced the expression of upstream DNA repair kinases ATM and ATR, which may in part explain our observation that EPZ015938 and the DNA-alkylating agent, melphalan, have combinatory effects. Of interest, using a low-dose of mTOR-inhibitor, we observed that cell viability was partially rescued from the effects of EPZ015938, indicating a role for mTOR-related pathways in the anti-myeloma activity of EPZ015938. Moreover, PRMT5 was shown to be involved in splicing regulation of MMSET and SLAMF7, known genes of importance in MM disease. As such, we broaden the understanding of the exact role of PRMT5 in MM disease and further underline its use as a possible therapeutic target.
Kinetics and Reaction Mechanism of Biothiols Involved in SNAr Reactions: An Experimental Study
Authors: Campodónico Paola R. , Alarcón-Espósito Jazmín , Olivares Belén
Published: June 2022

Few kinetic parameters, or reaction rates, are known up to date in detail about 1-chloro and 1-fluoro-2,4-dinitrobenzene (ClDNB and FDNB, respectively) with a series of biothiols in aqueous media. These biological nucleophiles with thiol groups have been widely used as a reference in nucleophile reactivity assays due to their prevalence and cellular abundance. The main aim of this study was to elucidate the reaction mechanism based on Brönsted-type plots and reactivity patterns of the electrophile/nucleophile pairs. A complete kinetic study was performed in terms of the comparison of Brönsted-type slope parameters (βnuc) for the reactions and was used for assigning the mechanism and the rate-determining step associated with the reaction route. A mass spectrometry analysis demonstrated that the nucleophilic center of the biothiols is the -SH group and there is only one kinetic product. The kinetic study suggests that the reaction mechanism might be the borderline between concerted and stepwise pathways. An amine–enol equilibrium for the most reactive nucleophiles appears to be the main determining factor controlling the nucleophilic attack in the nucleophilic aromatic substitution reactions investigated, highlighting the anionic form for these nucleophiles. This amine–enol equilibrium involves a hydrogen bond which stabilizes the intermediate species in the reaction pathway. Thus, intramolecular bonds are formed and enhance the nucleophilic strength through the contribution of the solvent surrounding the electrophile/nucleophile pairs. Finally, we highlight the importance of the formation of electrophile/nucleophile adducts that could modify structures and/or functions of biological systems with potential toxic effects. Therefore, it is essential to know all these kinetic and reactivity patterns and their incidence on other studies.
Mammoth Fauna Remains From Late Pleistocene Deposits of the Dmitry Laptev Strait South Coast (Northern Yakutia, Russia)
Authors: Kuznetsova Tatiana V. , Wetterich Sebastian , Matthes Heidrun , Tumskoy Vladimir E. , Schirrmeister Lutz
Published: June 2022

The Yedoma Ice Complex in northern Yakutia provides perfect preservation conditions for frozen remains of vertebrate animals. Even complete mummified specimens of the late Pleistocene Beringian Mammoth fauna such as woolly mammoth, woolly rhinoceros, horse, and bison are occasionally found in permafrost deposits across eastern Siberia, i.e., in West Beringia, although bones are much more commonly found. The present study characterizes mammal bones from late Pleistocene and Holocene permafrost deposits exposed on the Oyogos Yar coast, part of the southern shore of the Dmitry Laptev Strait that connects the Laptev and East Siberian seas. The study applies a method to characterize fossil bone samples by the location of their discovery and by the accuracy of their relation to a depositional horizon. We analyzed a total of 38 finite radiocarbon ages of bone material from mammoth, horse, and musk ox, spanning from about 48.8 to 4.5 ka BP and including both our own data and data from the literature, in addition to previous publications that reported numerous bones with infinite ages from the Oyogos Yar coast. The distribution of bones and tooth along the coastal permafrost exposure is not uniform; it depends upon whether the material was found in situ, on thermo-terraces, or on the shore. The overall bone collection consists of 13 species of which Mammuthus primigenius (woolly mammoth, 41%), Bison priscus (bison, 19%), Equus ex gr., caballus (horse, 19%), and Rangifer tarandus (reindeer, 16%) predominate. The fossil bone species distribution is similar to those of other prominent Yedoma outcrops in the region, i.e., on Bykovsky Peninsula and on Bol’shoy Lyakhovsky Island. Correlation analysis shows that the Oyogos Yar bone sampling sites of different geomorphological settings are similar to each other but not to all sampling sites within the other two locations on Bykovsky Peninsula and on Bol’shoy Lykahovsky Island. High similarities in terms of correlation coefficients between specific sampling sites are often not represented in the cluster analysis.
The Alteration of m6A Modification at the Transcriptome-Wide Level in Human Villi During Spontaneous Abortion in the First Trimester
Authors: She Jiajie , Tan Kaifen , Liu Jie , Cao Shuo , Li Zengguang , Peng You , Xiao Zhuoyu , Diao Ruiying , Wang Liping
Published: June 2022

A growing number of studies have demonstrated that N6 methyladenine (m6A) acts as an important role in the pathogenesis of reproductive diseases. Therefore, it is essential to profile the genome-wide m6A modifications such as in spontaneous abortion. In this study, due to the trace of human villi during early pregnancy, we performed high-throughput sequencing in villous tissues from spontaneous abortion (SA group) and controls with induced abortion (normal group) in the first trimester. Based on meRIP-seq data, 18,568 m6A peaks were identified. These m6A peaks were mainly located in the coding region near the stop codon and were mainly characterized by AUGGAC and UGGACG motif. Compared with normal group, the SA group had 2,159 significantly upregulated m6A peaks and 281 downregulated m6A peaks. Biological function analyses revealed that differential m6A-modified genes were mainly involved in the Hippo and Wnt signaling pathways. Based on the conjoint analysis of meRIP-seq and RNA-seq data, we identified thirty-five genes with differentially methylated m6A peaks and synchronously differential expression. And these genes were mainly involved in the Wnt signaling pathway, phosphatase activity regulation, protein phosphatase inhibitor activity, and transcription inhibitor activity. This study is the first to profile the transcriptome-wide m6A methylome in spontaneous abortion during early pregnancy, which provide novel insights into the pathogenesis and treatment of spontaneous abortion in the first trimester.
Immunophenotypic Landscape and Prognosis-Related mRNA Signature in Diffuse Large B Cell Lymphoma
Authors: Jiang Yanan , Sun Huimeng , Xu Hong , Hu Xin , Wu Wenqi , Lv Yangyang , Wang Jinhuan , Liu Su , Zhai Yixin , Tian Linyan , Wang Yafei , Zhao Zhigang
Published: June 2022

Diffuse large B cell lymphoma (DLBCL) exhibits a tightly complexity immune landscape. In this study, we intended to identify different immune phenotype and to examine the immune related mRNA signature for clinical characteristic, therapeutic responsiveness as well as risk stratification and survival prediction in DLBCL. We identified two immune infiltration subtypes of DLBCL patients based on 28 immune cell types. GSEA analysis uncovered the concordant classification of two robust significant subtypes of DLBCL. Considering the convenient application of the immune infiltration subtypes for prognostic prediction, we developed a risk score based on the differentially expressed genes between the Immunity-H and Immunity-L groups. By a least absolute shrinkage and selection operator (LASSO)-Cox regression model, a sixteen-gene risk signature, comprising ANTXR1, CD3D, TIMP1, FPR3, NID2, CTLA4, LPAR6, GPR183, LYZ, PTGDS, ITK, FBN1, FRMD6, PLAU, MICAL2, C1S, was established. The comprehensive results showed that the high-risk group was correlated with lower immune infiltration, more aggressive phenotypes, lower overall survival and more sensitive to lenalidomide. In contrast, a low-risk group score was associated with higher immune infiltration, less aggressive phenotypes, better overall survival and more likely to benefit from PD-1/PD-L1 inhibitors. Finally, a nomogram comprised of the risk score and IPI score was verified to more accurately predict the overall survival of DLBCL than traditional clinical prediction models. Altogether, our data demonstrate the heterogeneity of immune patterns within DLBCL and deepen our molecular understanding of this tumor entity.
Construction of Immune-Related ceRNA Network in Dilated Cardiomyopathy: Based on Sex Differences
Authors: Liu Chang , Liu Jian , Wu Daihong , Luo Shaoling , Li Weijie , Chen Lushan , Liu Zhen , Yu Bingbo
Published: June 2022

Background: Immune targeted therapy has become an attractive therapeutic approach for patients with dilated cardiomyopathy (DCM) recently. Genetic predisposition and gender play a critical role in immune-related responses of DCM. This study aimed to perform a bioinformatics analysis of molecular differences between male and female samples and identify immune-related ceRNA network in DCM.

Methods: The gene expression microarray and clinical features dataset of GSE19303 was downloaded from the GEO. The raw data were preprocessed, followed by identification of differentially expressed genes (DEGs) between male and female DCM samples. Crucial functions and pathway enrichment analysis of DEGs were investigated through GO analysis and KEGG pathway analysis, respectively. A lncRNA–miRNA–mRNA network was constructed and a central module was extracted from the ceRNA network.

Results: Compared with the female group, the male group benefits more from IA/IgG immunotherapy. Male patients of DCM had a significant positive correlation with the abundance of inflammatory cells (B cells, memory B cells, CD8+ Tem cells, and NK cells). Sex difference DEGs had a widespread impact on the signaling transduction, transcriptional regulation, and metabolism in DCM. Subsequently, we constructed an immune-related ceRNA network based on sex differences in DCM, including five lncRNAs, six miRNAs, and 29 mRNAs. Furthermore, we extracted a central module from the ceRNA network, including two lncRNAs (XIST and LINC00632), three miRNAs (miR-1-3p, miR-17-5p, and miR-22-3p), and six mRNAs (CBL, CXCL12, ESR1, IGF1R, IL6ST, and STC1). Among these DEGs, CBL, CXCL12, and IL6ST expression was considered to be associated with inflammatory cell infiltration in DCM.

Conclusions: The identified ceRNA network and their enriched pathways may provide genetic insights into the phenotypic diversity of female and male patients with DCM and may provide a basis for development of sex-related individualization of immunotherapy.
TMED2/9/10 Serve as Biomarkers for Poor Prognosis in Head and Neck Squamous Carcinoma
Authors: Gao Wen , Zhang Zhe-Wen , Wang Hong-Yi , Li Xin-Di , Peng Wei-Ting , Guan Hao-Yu , Liao Yu-Xuan , Liu An
Published: June 2022

Background: Head and neck squamous carcinoma (HNSC) is one of the most common malignant tumors with high incidence and poor prognosis. Transmembrane emp24 structural domain (TMED) proteins are involved in protein transport and vesicle budding processes, which have implicated various malignancies’ progression. However, the roles of TMEDs in HNSC, especially in terms of development and prognosis, have not been fully elucidated.

Methods: We applied TIMER 2.0, UALCAN, GEPIA 2, Kaplan-Meier plotter, GEO, The Human Protein Atlas (HPA), cBioPortal, Linkedomics, Metascape, GRNdb, STRING, and Cytoscape to investigate the roles of TMED family members in HNSC.

Results: Compared with normal tissues, the mRNA expression levels of TMED1/2/4/5/7/8/9/10 were significantly increased in the TCGA HNSC dataset. And we combined GEPIA 2 and Kaplan-Meier Plotter to select TMED2/9/10 with prognostic value. Then we detected the levels of mRNA in the GEO HNSC database and the protein expression in HPA. It was found that the mRNA and protein expression levels of TMED2/9/10 were increased in HNSC. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that TMED2/9/10 and their co-expressed genes promoted the malignant behavior of tumors by participating in biological processes such as intracellular transferase complex, protein transport, focal adhesion, intracellular protein processing. Single-cell analysis and immune infiltration analysis suggested that immune responses of cancer-associated fibroblasts and endothelial cells might be associated with prognosis. Finally, the transcription factors-genes network and protein-protein functional interaction network pointed to genes such as X-box binding protein 1 (XBP1) and TMED7, which might cooperate with TMED2/9/10 to change the progression of HNSC.

Conclusions: Our study implied that TMED2/9/10 and related genes mightjointly affect the prognosis of HNSC, providing specific clues for further experimental research, personalized diagnosis strategies, and targeted clinical therapy for HNSC.
Two-Year Event-Free Survival Prediction in DLBCL Patients Based on In Vivo Radiomics and Clinical Parameters
Authors: Ritter Zsombor , Papp László , Zámbó Katalin , Tóth Zoltán , Dezső Dániel , Veres Dániel Sándor , Máthé Domokos , Budán Ferenc , Karádi Éva , Balikó Anett , Pajor László , Szomor Árpád , Schmidt Erzsébet , Alizadeh Hussain
Published: June 2022

Purpose

For the identification of high-risk patients in diffuse large B-cell lymphoma (DLBCL), we investigated the prognostic significance of in vivo radiomics derived from baseline [18F]FDG PET/CT and clinical parameters.

Methods

Pre-treatment [18F]FDG PET/CT scans of 85 patients diagnosed with DLBCL were assessed. The scans were carried out in two clinical centers. Two-year event-free survival (EFS) was defined. After delineation of lymphoma lesions, conventional PET parameters and in vivo radiomics were extracted. For 2-year EFS prognosis assessment, the Center 1 dataset was utilized as the training set and underwent automated machine learning analysis. The dataset of Center 2 was utilized as an independent test set to validate the established predictive model built by the dataset of Center 1.

Results

The automated machine learning analysis of the Center 1 dataset revealed that the most important features for building 2-year EFS are as follows: max diameter, neighbor gray tone difference matrix (NGTDM) busyness, total lesion glycolysis, total metabolic tumor volume, and NGTDM coarseness. The predictive model built on the Center 1 dataset yielded 79% sensitivity, 83% specificity, 69% positive predictive value, 89% negative predictive value, and 0.85 AUC by evaluating the Center 2 dataset.

Conclusion

Based on our dual-center retrospective analysis, predicting 2-year EFS built on imaging features is feasible by utilizing high-performance automated machine learning.
Serum Total Bile Acids in Relation to Gastrointestinal Cancer Risk: A Retrospective Study
Authors: Li Songbo , Qu Xiaodong , Zhang Luyao , Wang Na , Chen Min , Zhao Xingyu , Wang Jie , Lv Huanhuan , Qi Ying , Zhang Lifeng , Liu Junye , Shi Yongquan
Published: June 2022

Background

Bile acids (BAs) have been proposed to promote gastrointestinal cells carcinogenesis. However, studies on serum total bile acid (TBA) levels and gastrointestinal cancers (GICs) risk are rare.

Methods

We conducted a retrospective case–control study from 2015 to 2019 at the First Affiliated Hospital of Air Force Military Medical University, in which 4,256 GICs cases and 1,333 controls were recruited. Patients’ demographic, clinical and laboratory data were collected. The odds ratios (ORs) with 95% confidence intervals (CIs) were estimated using binary logistic regression models.

Results

Positive associations were observed between serum TBA levels and risks of esophageal cancer (EC), gastric cancer (GC) and colorectal cancer (CRC). Overall, ORs of EC, GC and CRC risk rose with the TBA levels increasing. After adjustment for potential confounders, the OR of TBA-positive for EC risk was 4.89 (95% CI: 3.20-7.49), followed by GC (OR: 3.92, 95% CI: 2.53-6.08), and CRC (OR: 3.32, 95% CI: 2.04-5.11). Patients aged 60 years or older have a higher risk of GICs, especially for EC patients. Males are associated with a higher risk of GC, while females are associated with a higher risk of CRC. Preoperative serum TBA positive and negative was significantly different in the presence or absence of hematogenous metastasis among EC patients (P=0.014), and lymph node metastasis among GC patients (P=0.018).

Conclusions

This retrospective study showed positive associations between serum TBA level and GICs risk, and a higher serum TBA level constitutes a risk factor for GICs.
PCF11, a Novel CD44-Downstream Transcriptional Target, Linking Its 3’-End Polyadenylation Function to Tumor Cell Metastasis
Authors: Al-Mansoob Maryam , Ahmad Salma M. S. , Ouhtit Allal
Published: June 2022

Breast Cancer (BC) is the most common and the major health issue in women worldwide. Metastasis, a multistep process, is the worst aspect of cancer and tumor cell invasion is the defining step. Tumor cell invasion requires cell adhesion molecules (CAMs), and alterations in CAMs is considered as an initiating event in metastasis. Among CAMs, CD44 is a large family of more than 100 isoform, and its precise function was initially controversial in BC. Therefore, we have previously established a (Tet)-off inducible expression system of CD44 in MCF-7 primary BC cell line, and showed that CD44 promoted BC invasion/metastasis both in vitro and in vivo. A microarray gene expression profiling revealed more than 200 CD44-downstream potential transcriptional target genes, mediating its role in BC cell invasion and metastasis. Among these CD44-target genes, the Pre-mRNA cleavage complex 2 protein (PCF11) was upregulated upon the activation of CD44 by its major ligand hyaluronan (HA); This prompted us to hypothesize PCF11 as a potential novel transcriptional target of CD44-promoted BC cell invasion and metastasis. A large body of evidence from the literature supports our hypothesis that CD44 might regulate PCF11 via MAPK/ERK pathway. This review aims to discuss these findings from the literature that support our hypothesis, and further provide possible mechanisms linking CD44-promoted cell invasion through regulation of its potential target PCF11.
Karyotype of the Blastocoel Fluid Derived by Laser-Assisted Hatching Demonstrates a Low Agreement With the Trophectoderm
Authors: Wang Liang , Pang Wenjuan , Zhang Yi , Hao Min , Liu Yan , Wang Xiang , Sun Ningxia
Published: June 2022

Objective

The aim of this study is to compare the amplification efficiency and the genomic profiles of blastocoel fluid (BF) derived by laser-assisted hatching and trophectoderm (TE) cells derived from the same blastocyst.

Methods

Fifty-four fresh blastocysts underwent shrinkage by laser-assisted hatching, and each BF sample was collected individually. BF and TE cells were retrieved from each blastocyst for chromosome analysis through multiple annealing and looping-based amplification cycles (MALBAC) and next-generation sequencing (NGS).

Results

Fifty-four BF samples and 32 TE samples were retrieved for this study. Out of the 54 BF samples, only 35 provided reliable NGS data for comprehensive chromosome analysis (64.8%), while all 32 TE samples did (100%). Finally, there were 23 pairs of BF and TE samples from the same blastocyst. Only 17.4% of the BF-DNA karyotypes were completely agreeable with the TE samples (4/23).

Conclusion

Blastocoel fluid derived by laser-assisted hatching is easy to operate, and BF-DNA can be successfully amplified and subjected to NGS. Due to the low amplification efficiency and increased discordance with TE, BF does not adequately represent the status of the rest of the blastocyst. The use of BF as a single source of DNA for preimplantation genetic screening (PGS) is not yet advised.
Small Molecule RPI-194 Stabilizes Activated Troponin to Increase the Calcium Sensitivity of Striated Muscle Contraction
Authors: Mahmud Zabed , Tikunova Svetlana , Belevych Natalya , Wagg Cory S. , Zhabyeyev Pavel , Liu Philip B. , Rasicci David V. , Yengo Christopher M. , Oudit Gavin Y. , Lopaschuk Gary D. , Reiser Peter J. , Davis Jonathan P. , Hwang Peter M.
Published: June 2022

Small molecule cardiac troponin activators could potentially enhance cardiac muscle contraction in the treatment of systolic heart failure. We designed a small molecule, RPI-194, to bind cardiac/slow skeletal muscle troponin (Cardiac muscle and slow skeletal muscle share a common isoform of the troponin C subunit.) Using solution NMR and stopped flow fluorescence spectroscopy, we determined that RPI-194 binds to cardiac troponin with a dissociation constant KD of 6–24 μM, stabilizing the activated complex between troponin C and the switch region of troponin I. The interaction between RPI-194 and troponin C is weak (KD 311 μM) in the absence of the switch region. RPI-194 acts as a calcium sensitizer, shifting the pCa50 of isometric contraction from 6.28 to 6.99 in mouse slow skeletal muscle fibers and from 5.68 to 5.96 in skinned cardiac trabeculae at 100 μM concentration. There is also some cross-reactivity with fast skeletal muscle fibers (pCa50 increases from 6.27 to 6.52). In the slack test performed on the same skinned skeletal muscle fibers, RPI-194 slowed the velocity of unloaded shortening at saturating calcium concentrations, suggesting that it slows the rate of actin-myosin cross-bridge cycling under these conditions. However, RPI-194 had no effect on the ATPase activity of purified actin-myosin. In isolated unloaded mouse cardiomyocytes, RPI-194 markedly decreased the velocity and amplitude of contractions. In contrast, cardiac function was preserved in mouse isolated perfused working hearts. In summary, the novel troponin activator RPI-194 acts as a calcium sensitizer in all striated muscle types. Surprisingly, it also slows the velocity of unloaded contraction, but the cause and significance of this is uncertain at this time. RPI-194 represents a new class of non-specific troponin activator that could potentially be used either to enhance cardiac muscle contractility in the setting of systolic heart failure or to enhance skeletal muscle contraction in neuromuscular disorders.
Effects of Parecoxib Sodium Application Combined with Enhanced Recovery After Surgery Nursing on Inflammatory Factors and Knee Joint Function in Elderly Patients After Total Knee Arthroplasty
Authors: Deng Liqiong , Tan Liping
Published: June 2022

Objectives

To study the effect of parecoxib sodium (PS) application, combined with enhanced recovery after surgery (ERAS) nursing, on inflammation and knee joint function in elderly patients after total knee arthroplasty (TKA).

Methods

In this prospective cross-sectional study, we recruited 120 elderly patients treated with TKA who were randomly divided into two groups, the combine group and the control group, with 60 patients in each group. Patients in the control group received ERAS nursing and normal saline, and the patients in the combine group received ERAS nursing and PS. At different times after surgery, we compared the hemoglobin (Hb), complete white blood cell count (WBC), erythrocyte sedimentation rate (ESR), and serum IL-1β, TNF-α, and IL-6, and recovery time for different ranges of joint motion and the knee joint function HSS (hospital for special surgery scale) score between the two groups.

Results

On the third and seventh postoperative days, the levels of Hb in the patients of the combine group were significantly lower than those in the control group (p < 0.05), while the levels of WBC, ESR, serum IL-1β, TNF-α, and IL-6 in the patients of the combine group were all significantly lower than those in the control group (p < 0.05). Compared with the patients in the control group, the recovery time for 30, 60, 90, and 120 angles of joint motion in patients of the combine group was significantly decreased (p < 0.05), and the HSS score of patients in the combine group was significantly higher than that in the control group on the first, third, and sixth postoperative months (p < 0.05).

Conclusion

Elderly TKA patients who received PS application, combined with ERAS nursing, had lower inflammation in peripheral blood 2 weeks after operation and faster postoperative recovery of knee joint function.
THE EFFECTIVENESS OF INTERVENTION MODEL ON GLAUCOMA HEALTH SERVICE ACCESS AMONG PEOPLE OVER 40 YEARS OLD IN HUE CITY
Authors: Tran Nguyen Tra My , Nguyen Minh Tam , Phan Van Nam
Published: June 2022

Introduction: Glaucoma is the leading cause of irreversible blindness and the second leading cause of blindness in general. Early detection and timely treatment help reduce the progression of the disease, indirectly limit the economic burden and ensure the quality of life for patients. Currently, access to glaucoma prevention medical services is difficult in developing countries due to limited access to eye care facilities. Objective: To assess the effectiveness of interventions to increase the access to glaucoma services of people over 40 years old in Hue city. Methods: Applied the study design for community intervention with the comparison between intervened and control groups. Results: Our intervention approach included 3 groups of activities: active communication to change behaviors, medical staff training to improve knowledge and practice, treatment and management for glaucoma patients, suspected glaucoma cases or those at risk factors for glaucoma. Results showed that, in the intervention group, the percentage of people with good knowledge increased from 2.5% to 49.1%, good attitude increased from 3.4% to 51.6%, good practice increased from 2.3% to 46.3%. Regarding the improvement of access to glaucoma services, the proportion of people having annual eye exams increased from 30.7% to 49.9%, the figure for having glaucoma screening increased from 26.9% to 59.0%, proportion of those who were glaucoma patients, suspected and at risk with glaucoma that regularly monitored is 90.8%, percentage of those completely followed medication used instruction is 60%. In terms of the effective of the intervention program, the change in glaucoma good practice is 43.2%,
the change in having annual eye examination is 46.7%, and the change in having glaucoma screening is 57.7%. Ability to access glaucoma screening services increased by 8 (95%CI: 5.31-12.04, p<0.05) times, good knowledge increased by 3.67 (95%CI: 1.47-9.1, p<0.05) times, good attitude increased by 2.13 (95%CI: 1.02-4.43, p<0.05) times and practice increased by 2.39 (95%CI: 1.42-1.56, p<0.05) times compared to the control groups. Conclusions: There is need to enhance the communication and education on glaucoma for the people, improve the knowledge and ability to detect glaucoma for local health care workers within the existing capacity of the primary health care facility, closely coordinate between medical facilities at different levels in providing glaucoma medical services for all people.
Key words: glaucoma, knowledge, attitude, practice, health service accessibility.
EVALUATION OF THE SURGERY OUTCOME OF PLEOMORPHIC ADENOMA OF THE PAROTID GLAND
Authors: Vo Khac Trang , Hoang Minh Phuong , Nguyen Van Minh , Tran Tan Tai
Published: June 2022

Background: Pleomorphic adenoma, accounting for 80% of all parotid benign tumors and associated with a high recurrence rate after surgery. Early diagnosis and effective treatment will contribute to reducing complications of surgery. Objective: Evaluation of surgical results of pleomorphic adenoma on facial paralysis and other complications. Materials and Methods: including 25 patients with pleomorphic adenoma who were operated at Hue University of Medicine and Pharmacy from January 2020 to December 2020 and evaluate complications after 1 week, 1 month, 3 months, and 6 months. Results: After surgery at 1 week, 1 month, 3 months, and 6 months, the rate of facial paralysis gradually decreased by 76%, 52%, 32%, 0%, respectively. After 6 months, 100% reaching grade I on the House-Brackmann scale. cervicofacial branches accounted for the highest rate (48%). Other common complications include numbness around the ear, hemorrhage, sialocele, salivary fistula. Nevertheless, no cases of tumor recurrence and Frey’s syndrome were recorded. Conclusion: Parotidectomy is an effective and safe treatment method of pleomorphic adenoma. Facial paralysis accounts for a high incidence, but it will fully recover after 6 months. Choosing the appropriate treatment method helps to minimize complications and recurrence after surgery for pleomorphic adenoma of the parotid gland.
Key words: Pleomorphic Adenoma, Parotidectomy, Facial paralysis