bluuebunny/crossref_metadata_2025 huggingface dataset:
What can intraspecific trait variability tell us about fungal communities and adaptations?
Authors: Krah Franz-Sebastian , Bässler Claus
Published: July 2021

Analyses of species functional traits are suitable to better understand the coexistence of species in a given environment. Trait information can be applied to investigate diversity patterns along environmental gradients and subsequently to predict and mitigate threats associated with climate change and land use. Species traits are used to calculate community trait means, which can be related to environmental gradients. However, while species traits can provide insights into the mechanisms underlying community assembly, they can lead to erroneous inferences if mean trait values are used. An alternative is to incorporate intraspecific trait variability (ITV) into calculating the community trait means. This approach gains increasing acceptance in plant studies. For macrofungi, functional traits have recently been applied to examine their community ecology but, to our knowledge, ITV has yet to be incorporated within the framework of community trait means. Here, we present a conceptual summary of the use of ITV to investigate the community ecology of macrofungi, including the underlying ecological theory. Inferences regarding community trait means with or without the inclusion of ITV along environmental gradients are compared. Finally, an existing study is reconsidered to highlight the variety of possible outcomes when ITV is considered. We hope this Opinion will increase awareness of the potential for within-species trait variability and its importance for statistical inferences, interpretations, and predictions of the mechanisms structuring communities of macro- and other fungi.
Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study
Authors: Coperchini Francesca , Ricci Gianluca , Croce Laura , Denegri Marco , Ruggiero Rubina , Villani Laura , Magri Flavia , Chiovato Luca , Rotondi Mario
Published: July 2021

Introduction

Angiotensin-converting-enzyme-2 (ACE-2) was demonstrated to be the receptor for cellular entry of SARS-CoV-2. ACE-2 mRNA was identified in several human tissues and recently also in thyroid cells in vitro.

Purpose

Aim of the present study was to investigate the effect of pro-inflammatory cytokines on the ACE-2 mRNA levels in human thyroid cells in primary cultures.

Methods

Primary thyroid cell cultures were treated with IFN-γ and TNF-α alone or in combination for 24 h. ACE-2 mRNA levels were measured by RT-PCR. As a control, the levels of IFN-γ inducible chemokine (CXCL10) were measured in the respective cell culture supernatants.

Results

The mean levels of ACE-2 mRNA increased after treatment with IFN-γ and TNF-α in all the thyroid cell preparations, while the combination treatment did not consistently synergically increase ACE-2-mRNA. At difference, CXCL10 was consistently increased by IFN-γ and synergically further increased by the combination treatment with IFN-γ + TNF-α, with respect to IFN-γ alone.

Conclusions

The results of the present study show that IFN-γ and, to a lesser extent TNF-α consistently increase ACE-2 mRNA levels in NHT primary cultures. More interestingly, the combined stimulation (proven to be effective according to the synergic effect registered for CXCL10) produces different responses in terms of ACE-2 mRNA modulation. These results would suggest that elevated levels of pro-inflammatory cytokines could facilitate the entering of the virus in cells by further increasing ACE-2 expression and/or account for the different degree of severity of SARS-COV-2 infection. This hypothesis deserves to be confirmed by further specific studies.
W246G Mutant ELOVL4 Impairs Synaptic Plasticity in Parallel and Climbing Fibers and Causes Motor Defects in a Rat Model of SCA34
Authors: Nagaraja Raghavendra Y. , Sherry David M. , Fessler Jennifer L. , Stiles Megan A. , Li Feng , Multani Karanpreet , Orock Albert , Ahmad Mohiuddin , Brush Richard S. , Anderson Robert E. , Agbaga Martin-Paul , Deák Ferenc
Published: July 2021

Spinocerebellar ataxia (SCA) is a neurodegenerative disorder characterized by ataxia and cerebellar atrophy. A number of different mutations gives rise to different types of SCA with characteristic ages of onset, symptomatology, and rates of progression. SCA type 34 (SCA34) is caused by mutations in ELOVL4 (ELOngation of Very Long-chain fatty acids 4), a fatty acid elongase essential for biosynthesis of Very Long Chain Saturated and Polyunsaturated Fatty Acids (VLC-SFA and VLC-PUFA, resp., ≥28 carbons), which have important functions in the brain, skin, retina, Meibomian glands, testes, and sperm. We generated a rat model of SCA34 by knock-in of the SCA34-causing 736T>G (p.W246G) ELOVL4 mutation. Rats carrying the mutation developed impaired motor deficits by 2 months of age. To understand the mechanism of these motor deficits, we performed electrophysiological studies using cerebellar slices from rats homozygous for W246G mutant ELOVL4 and found marked reduction of long-term potentiation at parallel fiber synapses and long-term depression at climbing fiber synapses onto Purkinje cells. Neuroanatomical analysis of the cerebellum showed normal cytoarchitectural organization with no evidence of degeneration out to 6 months of age. These results point to ELOVL4 as essential for motor function and cerebellar synaptic plasticity. The results further suggest that ataxia in SCA34 patients may arise from a primary impairment of synaptic plasticity and cerebellar network desynchronization before onset of neurodegeneration and progression of the disease at a later age.
APPARILLO: a fully operational and autonomous staring system for LEO debris detection
Authors: Wagner Paul , Clausen Tim
Published: July 2021

For safe operation of active space crafts, the space debris population needs to be continuously scanned, to avoid collisions of active satellites with space debris. Especially the low Earth orbit (LEO) shows higher risks of collisions due to the highest density of orbital debris. Laser ranging stations can deliver highly accurate distance measurements of debris objects allowing precise orbit determination and more effective collision avoidance. However, a laser ranging station needs accurate a priori orbit information to track an orbital object. To detect and track unknown orbital objects in LEO, here, a passive optical staring system is developed for autonomous 24/7 operation. The system is weather-sealed and does not require any service to perform observations. To detect objects, a wide-angle imaging system with 10° field of view equipped with an astronomical CCD camera was designed and set up to continuously observe the sky for LEO objects. The system can monitor and process several passing objects simultaneously without limitations. It automatically starts an observation, processes the images and saves the 2D angular measurements of each object as equatorial coordinates in the TDM standard. This allows subsequent initial orbit determination and handover to a laser tracking system. During campaigns at twilight the system detected up to 36 objects per hour, with high detection efficiencies of LEO objects larger than 1 m3. It is shown that objects as small as 0.1 m3 can be detected and that the estimated precision of the measurements is about 0.05° or 7 × the pixel scale.
Prophylactic cholecystectomy is not mandatory in patients candidate to the resection for small intestine neuroendocrine neoplasms: a propensity score-matched and cost-minimization analysis
Authors: Ingaldi Carlo , Alberici Laura , Ricci Claudio , Campana Davide , Mosconi Cristina , Ambrosini Valentina , Lamberti Giuseppe , Manuzzi Lisa , Minni Francesco , Casadei Riccardo
Published: July 2021

To evaluate two competitive strategies in patients undergoing resection of Small-intestine neuroendocrine neoplasms (Si-NEN): prophylactic cholecystectomy (PC) versus On-demand delayed (OC) cholecystectomy. None comparative studies are available. This is a retrospective study based on 247 Si-NENs candidates for the primary tumor resection. Patients were divided into two arms: PC and OC. Propensity score matching was performed, reporting the d value. The primary outcome was the rehospitalization rate for any cause. The secondary endpoints were: the rehospitalization rate for biliary stone disease (BSD), the mean number of rehospitalization (any cause and BSD), the complication rate (all and severe). A P value < 0.05 was considered significant, and the number needed to treat (NNT) < 10 was considered clinically relevant. Before matching, 52 (21.1%) were in the PC arm and 195 (78.9%) in the OC group. The two arms have a sub-optimal balance for age (d = 0.575), symptoms (d = 0.661), ENETS TNM stage (d = 0.661). After matching, we included 52 patients in PC and 104 in OC one. The two groups are well balanced (all d values < 0.5). The rehospitalization rate was similar in the two groups (36% vs 31; P = 0.594; NNT = 21). The rehospitalization rate for BSD was lower in the PC arm than OC one (0% vs 7%) without statistical significance (P = 0.096) and relevance (NNT = 15). The mean number of readmission (any cause and BSD) and the complication rate (all and severe) were similar. PC was not mandatory in patients having Si-NEN and candidates to the resection of primary tumors.
Collateral Damage: Reuse in the Arts and the New Role of Quotation Provisions in Countries with Free Use Provisions After the ECJ’s Pelham, Funke Medien and Spiegel Online Judgments
Authors: Hui Alan , Döhl Frédéric
Published: July 2021

This article examines the impact of the European Court of Justice’s Pelham decision (C-476/17) on reuse, including appropriation art, borrowing and plagiarism in the arts, especially in music. Insofar, the focus lies on countries that have operated before with broad free use provisions. Specifically, we consider the extent to which EU law permits quotation provisions to fill the gap left by free use limitations, which have been curtailed by the Pelham decision. As we explain, Pelham creates a more restrictive approach to certain unlicensed use of copyright materials in new works of artistic expression, including music appropriation. We present our research in four sections. First, we compare existing national free use and quotation provisions in four states subject to EU law regarding their respective wiggle room for unlicensed yet lawful reuse in the arts. Second, we explore how the ECJ’s interpretation of the InfoSoc quotation exception, particularly in the Pelham, Funke Medien and Spiegel Online judgments, minimises the leeway for reuse in the arts provided by these national quotation provisions, in comparison to free use provisions. (Information Society Directive, 2001/29/EC.) Third, we address possible objections to our position and explain why we believe the consequences of the ECJ decisions cannot be bypassed. (Copyright in the Digital Single Market Directive, (EU) 2019/790.) Finally, in the conclusion, we explore the consequences, including the need for legislative reform.
Stability and Settlement Analysis of a Lightweight Embankment Filled with Waste Tyre Bales over Soft Ground
Authors: Duda Aleksander , Siwowski Tomasz W.
Published: July 2021

The waste tyre-derived products, including whole tyres, tyre bales, shreds, chips, and crumb rubber, have begun to be used in various geotechnical applications. In particular, the use of tyre bales in the construction of a lightweight embankment on the soft ground has the potential to satisfy the demand for low-cost materials exhibiting such beneficial properties. This paper presents the comparison between the common medium sand-filled embankment and two tyre-baled structures with various granular interlayers: medium sand and rubber aggregate. To assess the efficiency of tyre bale application in soft ground conditions, two subsoils were considered in the study: sandy clay and silty clay. The stability and settlement analysis of embankments, as well as subsoil bearing capacity checking, were performed for all structural cases. Bishop’s limit equilibrium slicing method and the finite element method were used in the embankment and subsoil analysis. The comprehensive testing of tyre bales and filling materials was also carried out to obtain the set of parameters used in both analyses. The comparison allowed qualitatively assessing the effectiveness of using waste tyre bales as a filling of road embankment when founded on soft ground. The analysis revealed that the application of tyre bales generally enhanced the embankment stability, effectively reduced the embankment settlement, and reduced the normal stress in the subsoil. In the tyre-baled embankments, the slip surface is located mostly within the embankment slope, showing good rotational stability, independent of subsoil conditions.
Management and outcome of metastatic pheochromocytomas/paragangliomas: a monocentric experience
Authors: De Filpo G. , Cantini G. , Rastrelli G. , Vannini G. , Ercolino T. , Luconi M. , Mannelli M. , Maggi M. , Canu L.
Published: July 2021

Background

Pheochromocytoma (PHEO) and paraganglioma (PGL) are rare neuroendocrine tumors releasing catecholamines. Metastatic pheochromocytomas/paragangliomas (PPGLs) occur in about 5–26% of cases. To date, the management of patients affected by metastatic disease is a challenge in the absence of guidelines.

Aim

The aim of this study was to evaluate the overall survival (OS) and the progression-free survival (PFS) in metastatic PPGLs.

Methods

Clinical data of 20 patients referred to the Careggi University Hospital (Florence, Italy) were retrospectively collected. Follow-up ranged from 1989 to 2019. Site and size of primary tumor, biochemical activity, genetic analysis and employed therapies were considered. Data were analyzed with SPSS version 27.

Results

Nine PHEOs (45%) and 11 PGLs (55%) were enrolled. Median age at diagnosis was 43.5 years [30–55]. Mean follow-up was 104.6 ± 89.3 months. Catecholamines were released in 70% of cases. An inherited disease was reported in 50% of patients. OS from the initial diagnosis (OSpt) and from the metastatic appearance (OSmtx) were lower in older patients (OSpt p = 0.028; OSmtx p < 0.001), abdominal PGLs (OSpt p = 0.007; OSmtx p = 0.041), larger tumors (OSpt p = 0.008; OSmtx p = 0.025) and sporadic disease (OSpt p = 0.013; OSmtx p = 0.008).

Conclusion

Our data showed that older age at the initial diagnosis, sympathetic extra-adrenal localization, larger tumors and wild-type neoplasms are related to worse prognosis. Notably, the employed therapies do not seem to influence the survival of our patients. At present, effective treatments for metastatic PPGLs are missing and a multidisciplinary approach is indispensably required.
Cosponsoring and Cashing In: US House Members’ Support for Punitive Immigration Policy and Financial Payoffs from the Private Prison Industry
Authors: Morín Jason L. , Torres Rachel , Collingwood Loren
Published: July 2021

The private prison industry is a multi-million-dollar industry that has increasingly profited from the detention of undocumented immigrants. As a government contractor, therefore, the industry has a natural interest in government decision making, including legislation that can affect its expansion into immigrant detention. In this article, we examine the relationship between campaign donations made on behalf of the private prison industry and an untested form of position taking—bill cosponsorship—in the US House of Representatives. We hypothesize the private prison industry will reward House members for taking positions that benefit the industry. We also hypothesize the private prison industry will also reward House members who incur greater political risk by taking positions out of sync with the party. To test our hypotheses, we focus on punitive immigration legislation that has the potential to increase the supply of immigrant detainees over the course of eight years. We find support for our second hypothesis, that private prison companies are more likely to reward House Democrats who cosponsor punitive immigration policies even after accounting for possible endogeneity. The findings have important implications regarding the relationship between House members and private interests.
The Veil of the COVID-19 Vaccination Certificates: Ignorance of Poverty, Injustice towards the Poor
Authors: GANTY Sarah
Published: July 2021

Socioeconomic disadvantages are amplified by the COVID-19 pandemic all over the world. Public actions and omissions severely affect the poor, alongside their precarious living, health and working conditions. As we slowly prepare for the aftermath of the pandemic, thanks to the progression of vaccination, especially in developed countries, certain measures taken in this context, more specifically the “vaccination certificates”, are likely particularly to affect the poor, who usually also belong to other vulnerable groups such as ethnic minorities or single parents, and could eventually constitute for them a state of permanent quarantine. In this contribution, I argue that COVID-19 vaccination certificates are a slippery slope towards exclusion and stigmatisation of the poor through a bureaucratic system based on privileges that raises important questions in light of the fundamental rights of the people who reside at society’s margins, struggling to conform to the “phantom of normalcy”.
Dielectric anisotropy as indicator of crystal orientation fabric in Dome Fuji ice core: method and initial results
Authors: Saruya Tomotaka , Fujita Shuji , Inoue Ryo
Published: July 2021

Polycrystalline ice is known to exhibit macroscopic anisotropy in relative permittivity (ɛ) depending on the crystal orientation fabric (COF). Using a new system designed to measure the tensorial components of ɛ, we investigated the dielectric anisotropy (Δɛ) of a deep ice core sample obtained from Dome Fuji, East Antarctica. This technique permits the continuous nondestructive assessment of the COF in thick ice sections. Measurements of vertical prism sections along the core showed that the Δɛ values in the vertical direction increased with increasing depth, supporting previous findings of c-axis clustering around the vertical direction. Analyses of horizontal disk sections demonstrated that the magnitude of Δɛ in the horizontal plane was 10–15% of that in the vertical plane. In addition, the directions of the principal axes of tensorial ɛ in the horizontal plane corresponded to the long or short axis of the elliptically elongated single-pole maximum COF. The data confirmed that Δɛ in the vertical and horizontal planes adequately indicated the preferred orientations of the c-axes, and that Δɛ can be considered to represent a direct substitute for the normalized COF eigenvalues. This new method could be extremely useful as a means of investigating continuous and depth-dependent variations in COF.
A Plague on Politics? The COVID Crisis, Expertise, and the Future of Legitimation
Authors: NEBLO MICHAEL A. , WALLACE JEREMY L.
Published: July 2021

Governments rely more and more on experts to manage the increasingly complex problems posed by a growing, diversifying, globalizing world. Surplus technocracy, however, usually comes with deficits of democracy. While especially true in liberal regimes, authoritarian states often face parallel dynamics. Recent trends illustrate how technocratic encroachment on civil society’s prerogatives can provoke populist backlash. Such cycles can build toward crises by eroding the legitimacy citizens invest in regimes. Surprisingly, by throwing both the need for and limits of expertise into sharp relief, the politics of COVID-19 create a novel opportunity to disrupt these trends. We assess how this opportunity may be unfolding in two crucial cases, the United States and China, and, more briefly, South Korea. We conclude by sketching some theoretical considerations to guide a geographically expanded and temporally extended research agenda on this important opportunity to slow or reverse a trend plaguing modern governance.
Impact of malnutrition on health-related quality of life in persons receiving dialysis: a prospective study
Authors: Viramontes-Hörner Daniela , Pittman Zoe , Selby Nicholas M. , Taal Maarten W.
Published: July 2021

Health-related quality of life (HRQoL) is severely impaired in persons receiving dialysis. Malnutrition has been associated with some measures of poor HRQoL in cross-sectional analyses in dialysis populations, but no studies have assessed the impact of malnutrition and dietary intake on change in multiple measures of HRQoL over time. We investigated the most important determinants of poor HRQoL and the predictors of change in HRQoL over time using several measures of HRQoL. We enrolled 119 haemodialysis and thirty-one peritoneal dialysis patients in this prospective study. Nutritional assessments (Subjective Global Assessment (SGA), anthropometry and 24-h dietary recalls) and HRQoL questionnaires (Short Form-36 (SF-36) mental (MCS) and physical component scores (PCS) and European QoL-5 Dimensions (EQ5D) health state (HSS) and visual analogue scores (VAS)) were performed at baseline, 6 and 12 months. Mean age was 64 (14) years. Malnutrition was present in 37 % of the population. At baseline, malnutrition assessed by SGA was the only factor independently (and negatively) associated with all four measures of HRQoL. No single factor was independently associated with decrease in all measures of HRQoL over 1 year. However, prevalence/development of malnutrition over 1 year was an independent predictor of 1-year decrease in EQ5D HSS, and 1-year decrease in fat intake independently predicted the 1-year decline in SF-36 MCS and PCS, and EQ5D VAS. These findings strengthen the importance of monitoring for malnutrition and providing nutritional advice to all persons on dialysis. Future studies are needed to evaluate the impact of nutritional interventions on HRQoL and other long-term outcomes.
Adhering to a Mediterranean diet in a Mediterranean country: an excess cost for families?
Authors: Alves Ricardo Mendes , Lopes Carla Maria Moura , Rodrigues Sara Simões Pereira , Perelman Julian
Published: July 2021

Adherence to the Mediterranean diet (MD) has been decreasing in southern Europe, which could be linked to several cultural or educational factors. Our aim is to evaluate the extent to which economic aspects may also play a role, exploring the relationship between food prices in Portugal and adherence to the MD. We evaluated data from the Portuguese National Food, Nutrition, and Physical Activity Survey (IAN-AF 2015–2016) (n3591). Diet expenditures were estimated by attributing a retail price to each food group, and the diet was transposed into the Mediterranean Diet Score used in the literature. Prices were gathered from five supermarket chains (65 % of the Portuguese market share). Linear regression models were used to assess the association between different adherence levels to the MD levels and dietary costs. Greater adherence to the MD was associated with a 21·2 % (P< 0·05) rise in total dietary cost, which accounts for more 0·59€ in mean daily costs when compared with low adherence. High adherence individuals (v. low adherence) had higher absolute mean daily costs with fish (0·62€/+285·8 %;P< 0·05), fruits (0·26€/+115·8 %;P< 0·05) and vegetables (0·10€/+100·9 %;P< 0·05). The analysis stratified by education and income level showed significantly higher mean daily diet cost only amongst higher income groups. Our findings suggest that greater adherence to the MD was positively and significantly associated with higher total dietary cost. Policies to improve population’s diet should take into consideration the cost of healthy foods, especially for large low- and middle-income families.
Glycaemic potency reduction by coarse grain structure in breads is largely eliminated during normal ingestion
Authors: SRV Akila , Mishra Suman , Hardacre Allan , Matia-Merino Lara , Goh Kelvin , Warren Frederick J. , Monro John Alexander
Published: July 2021

The hypothesis that coarse grain particles in breads reduce glycaemic response only if the particles remain intact during ingestion was tested. Three breads were formulated: (1) White bread (WB – reference), (2) 75 % of kibbled purple wheat in 25 % white bread matrix (PB) and (3) a 1:1 mixture of 37·5 % kibbled soya beans and 37·5 % of kibble purple wheat in 25 % white bread matrix (SPB). Each bread was ingested in three forms: unchewed (U), as customarily consumed (C) and homogenised (H). Twelve participants ingested 40 g available carbohydrate portions of each bread in each form, with post-prandial blood glucose measured over 120 min. Glycaemic responses to WB were the same regardless of its form when ingested. Unchewed PB had significantly less glycaemic effect than WB, whereas the C and H forms were similar to WB. Based on a glycaemic index (GI) of 70 for WB, the GI values for the C, U and H breads, respectively, were WB: 70·0, 70 and 70, PB: 75, 42 and 61, SPB: 57, 48 and 55 (%) (Least significant difference = 17·43, P < 0·05, bold numbers significantly different from WB). The similar glycaemic response to the H and C forms of the breads, and their difference from the U form, showed that the glycaemia-moderating effect of grain structure on starch digestion was lost during customary ingestion of bread. We conclude that the kibbled-grain structure may not effectively retard starch digestion in breads as normally consumed because it is largely eliminated by ingestive processes including chewing.
Who Supplies PPP Loans (and Does It Matter)? Banks, Relationships, and the COVID Crisis
Authors: Li Lei , Strahan Philip E.
Published: July 2021

We analyze the bank supply of credit under the Paycheck Protection Program (PPP). The literature emphasizes relationships as a means to improve lender information, which helps banks manage credit risk. Despite imposing no risk, however, the PPP supply reflects traditional measures of relationship lending: decreasing in bank size and increasing in prior experience, commitment lending, and core deposits. Our results suggest a new benefit of bank relationships: They help firms access government-subsidized lending. Consistent with this benefit, we show that the bank PPP supply, based on the structure of the local banking sector, alleviates increases in unemployment.
A ‘chemical help’ in middle-ear surgery? A systematic review on sodium 2-mercaptoethanesulfonate
Authors: Moffa A , Fiore V , Sabatino L , Cassano M , Tarabichi M , Kapadia M , Rinaldi V , Casale M
Published: July 2021

Objective

Sodium 2-mercaptoethanesulfonate (Mesna) has been proposed as a chemical aid in any surgical procedure, including cholesteatoma surgery. This review investigated the benefits and safety of Mesna during surgical management of cholesteatoma and adhesive otitis media.

Method

A systematic literature review was performed to identify clinical studies evaluating topical Mesna application during ear surgery (cholesteatoma or atelectasis). A qualitative analysis based on data extracted was conducted.

Results

From 27 articles, 5 retrospective studies were selected for a full analysis for a total of 607 patients (aged 5 to 72 years). Three studies evaluated cholesteatoma recidivism after Mesna application during cholesteatoma surgery, one study evaluated the surgical success rate of Mesna application for the treatment of atelectatic ears and adhesive otitis media, and one study evaluated potential ototoxicity of Mesna during cholesteatoma surgery. All the studies showed overall improvement in recurrence and residual cholesteatoma disease after Mesna application during surgery. Sensorineural hearing loss was not encountered after Mesna application.

Conclusion

Mesna application in cholesteatoma surgery could represent a valid and safe support tool during surgical treatment carried out both with microscopy and endoscopy. More studies are required to confirm these promising results.
Twenty-year experience with salvage total laryngectomy: lessons learned
Authors: Tsetsos N , Poutoglidis A , Vlachtsis K , Stavrakas M , Nikolaou A , Fyrmpas G
Published: July 2021

Objective

The purpose of this study was to evaluate the outcome of salvage total laryngectomy and identify areas for further improvement.

Method

A retrospective analysis of all patients who underwent salvage total laryngectomy between January 1999 and December 2018 was performed.

Results

Thirty-one patients were identified. The most common primary tumour site was the glottis (83.8 per cent). Early stage (T1–T2) disease was identified in 83.9 per cent of cases. Overall survival at 2 and 5 years post-salvage total laryngectomy was 71 per cent and 45 per cent, respectively. Disease-free survival at 2 and 5 years post-salvage total laryngectomy was 65 per cent and 42 per cent, respectively. The rate of post-salvage total laryngectomy pharyngocutaneous fistula was 29 per cent.

Conclusion

More than half of patients will not survive beyond five years after salvage total laryngectomy. Regional recurrence was the most common form of failure and death. From this study, elective lateral and central neck dissection is advocated in patients with early laryngeal cancer who present with an advanced recurrence.
Du Bois’s Century
Authors: Katznelson Ira
Published: July 2021

In March 1900, William Edward Burghart Du Bois addressed the third annual meeting of the American Negro Academy on “the present outlook for the dark races of mankind.” He cautioned, though

It is natural for us to consider that our race question is a purely national and local affair, confined to nine million Americans and settled when their rights and opportunities are assured. … a glance over the world at the dawn of a new century will convince us that this is but the be-ginning of the problem—that the color line belts the world and that the social problem of the twentieth century is to be the relation of the civilized world to the dark races of mankind. If we start eastward tonight and land on the continent of Africa we land in the center of the greater Negro problem—of the world problem of the black man. (1996 [1900]: 47–48)
Taxing sugar-sweetened beverages as a policy to reduce overweight and obesity in countries of different income classifications: a systematic review
Authors: Itria Alexander , Borges Stéfani S. , Rinaldi Ana Elisa M , Nucci Luciana Bertoldi , Enes Carla Cristina
Published: July 2021

Objective:

To evaluate the potential impact of sugar-sweetened beverage (SSB) taxes on overweight and obesity prevalence in countries of different income classifications.

Design:

Systematic review following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines (PROSPERO number CRD42020161612). Five databases (Cochrane Library, Embase, LILACS (via Virtual Health Library) and MEDLINE (via PubMed), and Web of Science were searched, from January 2009 to December 2019. Articles that reported changes in purchases, sales, intake, body weight, BMI, overweight and/or obesity prevalence due to a tax on or price change in SSB were included.

Setting:

Studies conducted in countries of different income classifications.

Participants:

The search yielded 8349 articles of which 21 met inclusion criteria.

Results:

Among the sixteen studies selected, only two did not show that consumption, sales and purchase decreased as the price of SSB increased. In eight of the thirteen studies selected, a positive effect of an SSB tax on decreasing overweight and obesity prevalence was expected. It is estimated that a 20 % taxation on SSB would result in a greater decrease in the prevalence of overweight and obesity compared to a 10 % rate. Studies with no significant effect of taxing on sales, purchases, consumption and prevalence of obesity were from high-income countries, while significant effects of taxing on reducing purchase, consumption and/or obesity prevalence were found in studies from upper-middle- and middle-income countries.

Conclusion:

A high SSB tax might be an effective fiscal policy to decrease purchase and consumption of SSB and reduce overweight/obesity prevalence, especially if the tax were specific for beverage volume.
On legal bubbles: some thoughts on legal shockwaves at the core of the digital economy
Authors: Giraudo Marco
Published: July 2021

For two decades, economic agents have been investing in the personal data-driven industry on the assumption that courts, the keepers of the legal systems, would have backed their claims, turning their technological control of personal data into legally protected property rights. Courts' backing has proved only temporary as they are now revising early solutions by constraining collection, use, and trade of personal data to protect the hierarchically superior rights. The theory of ‘legal bubbles’ rationalizes the economics underlying the legal shockwaves affecting the personal data-driven economy. ‘Legal bubbles’ tend to arise when economic agents invest in the economic exploitation of a new resource in a context of uncertainty and ignorance about the legal implications of innovative activities. These legal foundations may eventually turn out to be unstable as a result of courts'ex-postattempt to re-adapt them to the previously ignored implications of unconstrained commodification, with disruptive economic consequences.
The Crustal Anisotropy of West Ordos Block and Its Geodynamic Implications
Authors: Lv Jinyu , Shen Xuzhang , Huang Liuting , He Xiaohui , Huang He , Zheng Wenjun , Zhang Dongli , Zhang Jian
Published: July 2021

Based on the teleseismic records of the dense broadband seismic array, the crustal anisotropy parameters of the west Ordos block and its adjacent regions were determined with P wave receiver functions. The results indicate that the dominant direction of the fast wave is NEE‐SWW, N‐S in Yingshan block and north Ordos block, N‐S in the south Ordos block, and E‐W in the Qinling–Dabie orogen belt. The fast wave direction of crustal anisotropy in the north Ordos block is distinctly different from that in the south Ordos block, both well related to the major geological events, such as the Paleoproterozoic continent‐to‐continent collision between the Ordos Block and the Yinshan Block, the collision between the Western Block and Eastern Block of the North China Craton and the material migration from the Tibetan plateau in Cenozoic. Our results indicate that the crustal anisotropy in the Ordos block preserves the traces of ancient tectonic movements. Combining the results of previous geophysical studies, we presented a crust‐mantle interaction model to explain the geophysical observations. The most prominent features of the model are the horizontal eastward expansion of the mantle material in the southern Ordos and the vertical upwelling of the mantle material in the northern Ordos. The different modes of movement of the mantle material led to the deep contrasting structures of north and south Ordos, including the crustal anisotropy. The mantle upwelling also implies that the north Ordos block might be currently experiencing craton destruction.
Cassini‐Plasma Interaction Simulations Revealing the Cassini Ion Wake Characteristics: Implications for In‐Situ Data Analyses and Ion Temperature Estimates
Authors: Holmberg M. K. G. , Cipriani F. , Nilsson T. , Hess S. , Huybrighs H. L. F. , Hadid L. Z. , Déprez G. , Wilson R. J. , Morooka M. W. , Felici M.
Published: July 2021

We have used Spacecraft Plasma Interaction Software (SPIS) simulations to study the characteristics (i.e., dimensions, ion depletion, and evolution with the changing spacecraft attitude) of the Cassini ion wake. We focus on two regions, the plasma disk at 4.5–4.7 RS, where the most prominent wake structure will be formed, and at 7.6 RS, close to the maximum distance at which a wake structure can be detected in the Cassini Langmuir probe (LP) data. This study also reveals how the ion wake and the spacecraft plasma interaction have impacted the Cassini LP measurements in the studied environments, for example, with a strong decrease in the measured ion density but with minor interference from the photoelectrons and secondary electrons originating from the spacecraft. The simulated ion densities and spacecraft potentials are in very good agreement with the LP measurements. This shows that SPIS is an excellent tool to use for analyses of LP data, when spacecraft material properties and environmental parameters are known and used correctly. The simulation results are also used to put constraints on the ion temperature estimates in the inner magnetosphere of Saturn. The best agreement between the simulated and measured ion density is obtained using an ion temperature of 8 eV at ∼4.6 RS. This study also shows that SPIS simulations can be used in order to better constrain plasma parameters in regions where accurate measurements are not available.
Evaluating the Forecast Impact of Assimilating ATOVS Radiance With the Regional System of Multigrid NLS‐4DVar Data Assimilation for Numerical Weather Prediction (SNAP)
Authors: Zhang Hongqin , Tian Xiangjun
Published: July 2021

The regional System of Multigrid Nonlinear Least Squares Four‐dimensional Variational Data Assimilation for Numerical Weather Prediction (SNAP) was recently established based on the multigrid NLS‐4DVar assimilation scheme, Weather Research and Forecasting numerical model, and Gridpoint Statistical Interpolation (GSI)‐based observation quality control and observation operator modules. The analysis variables are model state variables, rather than the control variables adopted in the conventional 4DVar system. The regional SNAP adopts the multigrid NLS‐4DVar, which can correct errors from large to small scales and accelerate iteration solutions, to minimize the cost function and obtain the optimal analysis. Therefore, the regional SNAP has a higher assimilation efficiency and accuracy. In addition, the assimilation performance of the regional SNAP for conventional and radar observations had been evaluated. The main goal of this study is to achieve the direct assimilation of satellite radiation data using the regional SNAP. In this study, 1‐week cycle assimilation experiments assimilating Advanced TIROS Operational Vertical Sounder (ATOVS) data were designed to fully evaluate the performance of regional SNAP compared with the GSI Ensemble Four‐dimensional Variational (4DEnVar) scheme. First, a rainstorm was selected to illustrate the performance of regional SNAP. The cumulative precipitation distribution of SNAP was closer to reality and the higher equitable threat score and lower far score indicate that the regional SNAP improves precipitation forecast. In the 1‐week numerical experiments, for the u/v wind and temperature variables, the regional SNAP outperforms GSI and there was an improvement in GSI for the humidity variable.
Does Photomineralization of Dissolved Organics Matter in Temperate Rivers?
Authors: Maavara Taylor , Logozzo Laura , Stubbins Aron , Aho Kelly , Brinkerhoff Craig , Hosen Jacob , Raymond Peter
Published: July 2021

Sunlight can oxidize dissolved organic carbon (DOC) to dissolved inorganic carbon (DIC) in freshwaters. The importance of complete photooxidation, or photomineralization, as a sink for DOC remains unclear in temperate rivers, as most estimates are restricted to lakes, high latitude rivers, and coastal river plumes. In this study, we construct a model representing over 75,000 river reaches in the Connecticut River Watershed (CRW), USA, to calculate spectrally resolved photomineralization. We test the hypothesis that photomineralization is a negligible DOC sink across all reaches and flow conditions relative to DOC fluxes. Our model quantifies reaction rates and transport drivers within the river reaches for the ranges of flow conditions, incoming solar irradiance, and canopy cover shading observed throughout the year. Our model predicts average daily areal photomineralization rates ranging from 1.16 mg‐C m−2 day−1 in low flow river reaches in the winter, to 18.33 mg‐C m−2 day−1 in high flow river reaches during the summer. Even for high photomineralization fluxes, corresponding photomineralization uptake velocities are typically at least an order of magnitude smaller than those reported for other instream processes. We calculate DOC elimination by photomineralization relative to DOC fluxes through individual stream reaches as well as the entire riverine portion of the CRW. We find that relative photomineralization fluxes are highest in summer drought conditions in low order streams. In median flows and mean light intensities, for an average watershed travel distance, 3%–5% of the DOC fluxes are eliminated, indicating that photomineralization is a minor DOC sink in temperate rivers.
High-Q microresonators on 4H-silicon-carbide-on-insulator platform for nonlinear photonics
Authors: Wang Chengli , Fang Zhiwei , Yi Ailun , Yang Bingcheng , Wang Zhe , Zhou Liping , Shen Chen , Zhu Yifan , Zhou Yuan , Bao Rui , Li Zhongxu , Chen Yang , Huang Kai , Zhang Jiaxiang , Cheng Ya , Ou Xin
Published: July 2021

The realization of high-quality (Q) resonators regardless of the underpinning material platforms has been a ceaseless pursuit, because the high-Q resonators provide an extreme environment for confining light to enable observations of many nonlinear optical phenomenon with high efficiencies. Here, photonic microresonators with a mean Q factor of 6.75 × 106 were demonstrated on a 4H-silicon-carbide-on-insulator (4H-SiCOI) platform, as determined by a statistical analysis of tens of resonances. Using these devices, broadband frequency conversions, including second-, third-, and fourth-harmonic generations have been observed. Cascaded Raman lasing has also been demonstrated in our SiC microresonator for the first time, to the best of our knowledge. Meanwhile, by engineering the dispersion properties of the SiC microresonator, we have achieved broadband Kerr frequency combs covering from 1300 to 1700 nm. Our demonstration represents a significant milestone in the development of SiC photonic integrated devices.
ErbB4 promotes inhibitory synapse formation by cell adhesion, independent of its kinase activity
Authors: Luo Bin , Liu Ziyang , Lin Dong , Chen Wenbing , Ren Dongyan , Yu Zheng , Xiong Mingtao , Zhao Changqin , Fei Erkang , Li Baoming
Published: July 2021

The precise control of the nervous system function under the vitality of synapses is extremely critical. Efforts have been taken to explore the underlying cellular and molecular mechanisms for synapse formation. Cell adhesion molecules have been found important for synapse assembly in the brain. Many trans-adhesion complexes have been identified to modulate excitatory synapse formation. However, little is known about the synaptogenic mechanisms for inhibitory synapses. ErbB4 is a receptor tyrosine kinase enriched in interneurons. Here, we showed that overexpressing ErbB4 in HEK293T cells induced gephyrin or GABAAR α1 puncta in co-cultured primary hippocampal neurons. This induction of ErbB4 was independent of its kinase activity. K751M, a kinase-dead mutant of ErbB4, can also induce gephyrin or GABAAR α1 puncta in the co-culture system. We further constructed K751M knock-in mice and found that the homozygous were viable at birth and fertile without changes in gross brain structure. The number of interneurons and inhibitory synapses onto pyramidal neurons (PyNs) were comparable between K751M and wild-type mice but decreased in ErbB4-Null mice. Moreover, ErbB4 can interact in trans with Slitrk3, a transmembrane postsynaptic protein at inhibitory synapses, through the extracellular RLD domain of ErbB4. The deletion of RLD diminished the induction of gephyrin or GABAAR α1 puncta by ErbB4. Finally, disruption of ErbB4–Slitrk3 interaction through neutralization of Slitrk3 by secretable RLD decreased inhibitory synapses onto PyNs and impaired GABAergic transmission. These results identify that ErbB4, as a cell adhesion molecule, promotes inhibitory synapse formation onto PyNs by interacting with Slitrk3 and in a kinase-independent manner, providing an unexpected mechanism of ErbB4 in inhibitory synapse formation.
Polygenic risk scoring to assess genetic overlap and protective factors influencing posttraumatic stress, depression, and chronic pain after motor vehicle collision trauma
Authors: Lobo Jarred J. , McLean Samuel A. , Tungate Andrew S. , Peak David A. , Swor Robert A. , Rathlev Niels K. , Hendry Phyllis L. , Linnstaedt Sarah D.
Published: July 2021

Posttraumatic stress (PTS), depressive symptoms (DS), and musculoskeletal pain (MSP) are common sequelae of trauma exposure. Although these adverse posttraumatic neuropsychiatric sequelae (APNS) are often studied separately, clinical comorbidity is high. In a cohort of European American motor vehicle collision (MVC) trauma survivors (n = 781), substantial PTS (≥33, IES-R), DS (≥26, CES-D), and MSP (≥4, 0–10 NRS) were identified via a 6-month survey. Genetic risk was estimated using polygenic risk scores (PRSs) calculated from the largest available GWAS datasets of PTSD, MDD, and back pain. We then assessed comorbidity and genetic risk influence for developing chronic PTS, DS, and MSP after MVC. Secondary analyses explored whether common social determinants of health ameliorate genetic vulnerability. We found that 6 months after MVC, nearly half 357/781 (46%) of the participants had substantial PTS, DS, and/or MSP, and overlap was common (PTS + MSP (23%), DS + MSP (18%), PTS + DS (12%)). Genetic risk predicted post-MVC outcomes. PTSD-PRSs predicted PTS and DS (R2 = 2.21% and 2.77%, padj < 0.01), MDD-PRSs predicted DS and MSP (R2 = 1.89%, padj < 0.01) and 0.79%, padj < 0.05), and back pain-PRS predicted MSP (R2 = 1.49%, padj < 0.01). Individuals in the highest quintile of PTSD-PRSs had 2.8 and 3.5 times the odds of developing PTS and DS vs. the lowest quintile (95% CI = 1.39–5.75 and 1.58–7.76). Among these high-risk individuals, those living in non-disadvantaged neighborhoods and with college education had 47% (p = 0.048) and 52% (p = 0.04) less risk of developing PTS, and those with high social support had 60% (p = 0.008) less risk of developing DS. Overall, genetic factors influence the risk of APNS after MVC, genetic risk of distinct APNS are overlapping, and specific social determinants greatly augment genetic risk of APNS development after MVC.
Circular RNA hsa_circ_0000277 sequesters miR-4766-5p to upregulate LAMA1 and promote esophageal carcinoma progression
Authors: Zhou Peng Li , Wu Zhengyang , Zhang Wenguang , Xu Miao , Ren Jianzhuang , Zhang Qinhui , Sun Zhanguo , Han Xinwei
Published: July 2021

Growing evidence has indicated that circular RNAs (circRNAs) play a pivotal role as functional RNAs in diverse cancers. However, most circRNAs involved in esophageal squamous cell carcinoma (ESCC) remain undefined, and the underlying molecular mechanisms mediated by circRNAs are largely unclear. Here, we screened human circRNA expression profiles in ESCC tissues and found significantly increased expression of hsa_circ_0000277 (termed circPDE3B) in ESCC tissues and cell lines compared to the normal controls. Moreover, higher circPDE3B expression in patients with ESCC was correlated with advanced tumor-node-metastasis (TNM) stage and dismal prognosis. Functional experiments demonstrated that circPDE3B promoted the tumorigenesis and metastasis of ESCC cells in vitro and in vivo. Mechanistically, bioinformatics analysis, a dual-luciferase reporter assay, and anti-AGO2 RNA immunoprecipitation showed that circPDE3B could act as a competing endogenous RNA (ceRNA) by harboring miR-4766-5p to eliminate the inhibitory effect on the target gene laminin α1 (LAMA1). In addition, LAMA1 was significantly upregulated in ESCC tissues and was positively associated with the aggressive oncogenic phenotype. More importantly, rescue experiments revealed that the oncogenic role of circPDE3B in ESCC is partly dependent on the miR-4766-5p/LAMA1 axis. Furthermore, bioinformatics analysis combined with validation experiments showed that epithelial-mesenchymal transition (EMT) activation was involved in the oncogenic functions of the circPDE3B–miR-4766-5p/LAMA1 axis in ESCC. Taken together, we demonstrate for the first time that the circPDE3B/miR-4766-5p/LAMA1 axis functions as an oncogenic factor in promoting ESCC cell proliferation, migration, and invasion by inducing EMT, implying its potential prognostic and therapeutic significance in ESCC.