bluuebunny/crossref_metadata_2025 huggingface dataset:
Smooth Information Criterion for Regularized Estimation of Item Response Models
Authors: Robitzsch Alexander
Published: April 2024

Item response theory (IRT) models are frequently used to analyze multivariate categorical data from questionnaires or cognitive test data. In order to reduce the model complexity in item response models, regularized estimation is now widely applied, adding a nondifferentiable penalty function like the LASSO or the SCAD penalty to the log-likelihood function in the optimization function. In most applications, regularized estimation repeatedly estimates the IRT model on a grid of regularization parameters λ. The final model is selected for the parameter that minimizes the Akaike or Bayesian information criterion (AIC or BIC). In recent work, it has been proposed to directly minimize a smooth approximation of the AIC or the BIC for regularized estimation. This approach circumvents the repeated estimation of the IRT model. To this end, the computation time is substantially reduced. The adequacy of the new approach is demonstrated by three simulation studies focusing on regularized estimation for IRT models with differential item functioning, multidimensional IRT models with cross-loadings, and the mixed Rasch/two-parameter logistic IRT model. It was found from the simulation studies that the computationally less demanding direct optimization based on the smooth variants of AIC and BIC had comparable or improved performance compared to the ordinarily employed repeated regularized estimation based on AIC or BIC.
Occupational Health and Safety Reporting in the Top 100 Australian Companies: Does Organisational Risk Profile Matter?
Authors: Oakman Jodi , Young Suzanne , Weale Victoria P. , Pattinson Alison
Published: April 2024

Increasingly, good quality and safe working conditions that promote employee health are expected by stakeholders. The aim of this study is to examine the extent and quality of occupational health and safety (OHS) reporting in the Top 100 companies listed on the Australian Stock Exchange (ASX). Method: Publicly available annual reports from the Top 100 ASX companies were reviewed using a policy scorecard against five dimensions drawn from the Australian Work Health and Safety Strategy 2012–2022. The dimensions were: OHS information, legislation, leadership, work health disorders, prevention and best practice. Results: Mean rank scores of high and low-risk industry sectors were compared. High-risk sectors provided more explicit coverage of OHS information across all five domains in comparison to low-risk sectors (p > 0.05). The Information Technology sector scored the lowest across all five dimensions. Conclusion: Higher quality reporting from those in high-risk sectors may be influenced by stakeholder expectations, as well as industry norms. The current analysis suggests that relying on stakeholders to drive improved reporting may be problematic, as those industries that are perceived to have a low OHS risk profile may not consider the need to provide transparent reporting on their strategies to ensure they are providing good quality working conditions.
Gender Disparity Index in European Former Communist States: Assessing the Influence of EU Membership
Authors: Pirju Ionel Sergiu , Panaitescu Manuela Carmen , Sorcaru Sergiu Lucian , Marinescu Liviu Mihail , Popa Daniela Aurelia Tanase
Published: April 2024

This research is a reflection on the new challenges in former European communist countries in three areas of gender equality. The analysis employs the gender disparity index (GDI), which encompasses life expectancy, education, and income indices, each equally weighted by gender. The former communist states are categorized into two clusters: the first comprising European Union (EU) member states, and the second consisting of non-EU member states or those in the integration process. The findings indicate pervasive gender-based inequalities across the former European communist bloc. This study offers critical insights for countries seeking EU accession, highlighting the need for targeted policies to address gender disparities in multiple domains. Contrary to the hypothesis positing a connection between feminine cultural values and gender egalitarian practices, the data do not support this assertion. The implications of this research are particularly pertinent for aspiring EU member states, who must navigate issues of monopolism while striving to establish effective principles of business efficiency.
Preliminary Design and Optimization of Primary Structures for a Tilt-Duct UAV
Authors: Xu Shangru , Liu Yaolong , Zhang Jifa , Zheng Yao
Published: April 2024

Tilt-duct Unmanned Aerial Vehicles (UAV) combine the high-speed efficiency of fixed-wing aircrafts with vertical takeoff and landing (VTOL) and the hover capabilities of rotary-wing aircrafts while maximizing the advantages of ducted fans in terms of noise reduction, efficiency, and safety, making it a pivotal direction for the future of aviation such as urban air mobility. This paper concentrates on the design and optimization of the primary structures of a laboratory-designed reference tilt-duct UAV. Firstly, the general data of the reference tilt-duct UAV are presented. According to the load conditions, the overall structural layout design for the wing, fuselage, and empennage is carried out, where special attention has been paid to account for the requirements of VTOL/hover and cruise flight modes. Based on the structural layout, finite element models (FEM) are established and static analyses are performed. The results indicate that the design can fulfill the structural requirements during a flight mission. Furthermore, based on the Method of Feasible Directions (MFD) algorithm, we have carried out the optimization of the composite wing box that incorporates manufacturing constraints. Via optimization, the total mass of the wing box is reduced by 38.6%, i.e., from 3.73 kg to 2.29 kg. The results indicate that the combination of composite materials with a tilt-duct configuration holds significant potential for future high-efficiency and environmentally friendly aviation.
Wastewater Nutrient Recovery via Fungal and Nitrifying Bacteria Treatment
Authors: Lopez Karla , Leme Vitoria F. C. , Warzecha Marcin , Davidson Paul C.
Published: April 2024

In efforts to reduce the consumption of fossil fuels and promote recycling biowaste, there is an interest in the production of post-hydrothermal liquefaction wastewater (HTL-AP) from the hydrothermal liquefaction (HTL) process that converts wet biomass into biocrude oil. This study explores ways of transforming potentially toxic HTL-AP into a fertilizer source for hydroponic cropping systems. This study specifically investigates the integration of the white-rot fungus Trametes versicolor with nitrifying bacteria (Nitrosomonas and Nitrobacter) to convert the organic nitrogen compounds into inorganic nitrogen while also producing the enzyme laccase, which has been shown to remove toxic compounds. This study aims to increase the concentration of nitrate-N to valorize wastewater as a suitable fertilizer by measuring several parameters, including laccase activity, pH, nitrate-N, and ammonia/ammonium-N concentrations, and analyzes interactions to optimize the conversion process. The data support the claim that the simultaneous inoculation of T. versicolor and nitrifying bacteria significantly increases nitrate-N concentrations in HTL-AP, as it increased by 17 times, or an increase of 32.69 mg/L. In addition, HTL-AP treated with T. versicolor and nitrifying bacteria reduced the treatment time by 120 h, highlighting a reduction in personnel time and energy consumption. Therefore, this research accentuates sustainability through fungal and bacterial treatments to develop eco-friendly hydroponic fertilizers. Future research should explore the potential of utilizing the combination of T. versicolor and nitrifying bacteria for the treatment of other industrial wastewaters.
Hot Water Treatment as a Quarantine Measure for Controlling Pratylenchus penetrans Cobb in Syngonium podophyllum Schott and Perilla frutescens Britton
Authors: Lim Ga-Eul , Heo Moon-Sun , Park Min-Goo
Published: April 2024

Syngonium podophyllum Schott plants are increasingly being imported in Korea due to their ability to purify indoor air. Root lesion nematodes, Pratylenchus spp., are the most frequently occurring nematodes associated with S. podophyllum, leading to the disposal of plants or their return to the country of origin, due to inadequate phytosanitary treatments. We evaluated the effectiveness of hot water treatment in controlling Pratylenchus penetrans Cobb, present in S. podophyllum and Perilla frutescens Britton. The mortality rate (LT99%: the time at which 99% lethality is achieved) of P. penetrans at 44 to 52 °C water and treatment-caused plant damage were evaluated in vitro and in vivo. The in vitro test showed the LT99 at 44, 46, 48, 50, and 52 °C was 119.1, 27.0, 14.70, 1.40, and 0.48 min, respectively. The LT99 of hot water treatment on the nematodes infecting both plants was 18.4 to 1.7 min at 46 to 50 °C. S. podophyllum was undamaged at 30 days after treatment with 49 and 51 °C water for 30 min. This study demonstrates that hot water treatment is a potential phytosanitary disinfection method for Pratylenchus spp. infecting foliage plants, which can accelerate their trade by reducing incidents of disposal or return to the country of origin.
Nosema apis and Nosema ceranae in Beehives of the Apulian Region of Italy: How Citizen Science Can Support Scientific Research
Authors: Pandiscia Annamaria , Lorusso Patrizio , Manfredi Alessio , Bonerba Elisabetta , Bozzo Giancarlo , Tantillo Giuseppina M. , Terio Valentina
Published: April 2024

Nosema apis and Nosema ceranae are two well-known pathogens affecting the health of honeybees. To help understand how honeybee colonies are affected by these pathogens, the aim of this study was to analyze the impact of Nosema ceranae and Nosema apis in hives in the Apulian Region through a Citizen Science approach. First, a form about the health status of the beehives was filled out by beekeepers. After an inspection visit to confirm the signs observed by beekeepers, adult honeybee samples collected from beehives in four Apulian provinces (Taranto, Bari, Foggia and Brindisi) were subjected to light microscopy investigation for the detection of Nosema spp. spores and to molecular analysis using species-specific primers for the discrimination of Nosema apis spores from those of Nosema ceranae. Among the forty-eight samples, thirty-six tested positive for Nosema ceranae, and one sample tested positive for Nosema apis. The forms filled out by beekeepers revealed that only 5/36 beehives that tested positive for Nosema ceranae showed signs of depopulation and reduced honey production, while 19/36 had only low honey yield. This study provides data on Nosema apis and Nosema ceranae prevalence in Italy and correlates the presence of these intestinal pathogens with the most important problems encountered by local beekeepers.
Design and Experiment of Plate Taking Control System of Edible Sunflower (Edulis helianthus catino L.) Harvester
Authors: Pan Feng , Chen Jincheng , Zhang Hui , Han Lin , Dong Yuncheng , Li Bin , Ji Chao
Published: April 2024

This study aims at the problems of high labor intensity, high cost and high loss rate of mechanical picking of seeds, low mechanization level and difficulty to guarantee the quality of picking plates in the process of picking edible sunflower. Based on the principle of manual plate taking, a plate taking control system for a sunflower harvesting table was designed. The principle of taking the plate of the edible sunflower harvesting table was analyzed. According to the actual operation requirements, the overall scheme of the sunflower plate control system is determined, and the control strategy of imitating artificial low-loss harvesting is designed. To reduce the grain loss in the process of taking the plate and improve the control accuracy of the system on the movement speed and displacement of the key components in the process of taking the plate, a trapezoidal acceleration and deceleration control algorithm is designed as the control algorithm of taking the plate. The working performance of the plate control system was verified with the absolute error, relative error and total loss rate of the harvest as objectives. Bench and field verification experiments were both carried out. The bench experiment showed that the speed error of the plate parts was not more than 0.028 m/s. In the bench experiments of the device, the maximum positioning error was 1.25 mm, the average relative error was only 0.94% and the grain loss rate was not more than 2.26%. Its result showed that the system algorithm was reliable, the positioning accuracy was high and the plate taking operation can be completed well. The field verification experiment showed that the forward speed of the unit was in the range of 0.4~0.8 m/s, and the total loss rate of harvest was less than 5%. When the forward speed is 0.6 m/s, the minimum harvest loss rate is 2.32%, which indicated the control system meets the requirements of sunflower harvesting operation.
Effects of Conventional Non-Biodegradable Film-Derived Microplastics and New Biodegradable Film-Derived Microplastics on Soil Properties and Microorganisms after Entering Sub-Surface Soil
Authors: Liu Xiaowei , Wei Wenliang , Liu Guocheng , Zhu Bo , Cui Jie , Yin Tao
Published: April 2024

Plastic film mulching, widely used in agriculture, leads to microplastic (MP) pollution in soils. While biodegradable polybutylene adipate terephthalate (PBAT) films may offer a solution, their impacts on subsurface soils and microorganisms remain unclear. To investigate the effects of conventional non-biodegradable polyethylene (PE) and biodegradable PBAT MPs on the properties of sub-surface soils and microbial communities, MPs were added at varying doses in a field experiment and incubated for 160 days. Physicochemical characteristics, nutrient dynamics, and microbial composition, diversity, and networks of soils were analyzed using standard techniques and 16S rRNA/ITS gene sequencing. Correlations between soil properties and microbes were assessed. Both MP types significantly altered soil characteristics, with PBAT-MP elevating pH and the levels of available phosphorus and potassium more than PE-MP. Microbial composition shifts occurred, with low-addition PBAT-MP promoting plastic-degrading genera. The assessment of α/β-diversity indicated that PBAT-MP predominantly influenced fungi while PE-MP impacted bacteria. An examination of microbial co-occurrence networks highlighted that PE-MP primarily disrupted fungal interactions, whereas PBAT-MP streamlined network complexity. Correlation analyses revealed that PBAT-MP promoted fungal diversity/network resilience correlating to nutrients. PE-MP and PBAT-MP significantly altered native soil/microbe relationships. PBAT-MP may exert greater, yet unknown, impacts over time through its biodegradation into newer and smaller fragments. Future research needs to integrate multi-omics and stable isotope science to elucidate the deep mechanistic impacts of degraded film-derived MPs on microbial ecological functions and biogeochemical cycles. Attention should also be paid to the long-term accumulation/transport of MPs in agricultural soils. Overall, this work deepens the impact and understanding of MPs from plastic film on sub-surface soil ecology. Furthermore, it provides a theoretical foundation for managing ‘white pollution’ in the film-covered farmlands of arid and semi-arid regions in China.
Sustainable Agriculture Practices: Utilizing Composted Sludge Fertilizer for Improved Crop Yield and Soil Health
Authors: Li Lijun , Li He , Tong Lihong , Lv Yizhong
Published: April 2024

It is desirable to recycle sewage sludge as fertilizer for agricultural fields. The application of sludge to agricultural soils is a measure that replaces chemical fertilizers and plays an important role in improving soil’s physicochemical and biological properties. However, there are concerns that the pollutants in sewage sludge will cause negative impacts on soil health. To closely monitor the soil–sludge interactions, a field study was conducted over a 20-year period in the North China Plain. In this study, the long-term effects of sewage sludge on the soil properties and soil microbial diversity were investigated. We examined the effects of various fertilization methods (control, chemical fertilizer, uncomposted sludge fertilizer, composted sludge fertilizer) on wheat production and several soil health indicators, such as the soil’s enzymatic activities, microbial biomass, microbial diversity, and crop yield. This long-term experiment supports that the composted sludge fertilizer increased crop production by 124.2% compared to the control treatment. The soil’s biological quality (e.g., the concentration of soil microbial biomass carbon) was also improved under the composted sludge fertilizer treatment. The concentrations of soil microbial biomass carbon under the uncomposted sludge fertilizer and composted sludge fertilizer treatments were 560.07 mg/kg and 551.07 mg/kg, respectively. The effect of the composted sludge fertilizer was greater than that of the uncomposted sludge fertilizer. The content of heavy metals did not exceed the national standard. The highest soil health index was 0.79 with the composted sludge fertilizer. Therefore, these results suggest that the application of composted sludge fertilizer has the potential to enhance long-term soil health and promote sustainable agricultural practices.
Drought Monitoring of Winter Wheat in Henan Province, China Based on Multi-Source Remote Sensing Data
Authors: Tian Guizhi , Zhu Liming
Published: April 2024

Characterized by soil moisture content and plant growth, agricultural drought occurs when the soil moisture content is lower than the water requirement of plants. Microwave remote sensing observation has the advantages of all-weather application and sensitivity to soil moisture change. However, microwave remote sensing can only invert 0~5 cm of soil surface moisture, so it cannot effectively reflect the drought situation of farmland. Therefore, this study took Henan Province as the study area, used soil moisture active and passive (SMAP) satellite soil moisture data, employed NDVI, LST, and ET as the independent variables, and took the drought grade on the sample as the dependent variable. Using the 2017–2019 data as the training set and the 2020 data as the testing set, a random forest drought monitoring model with comprehensive influence of multiple factors was constructed based on the training set data. In the process of model training, the cross-validation method was employed to establish and verify the model. This involved allocating 80% of the sample data for model construction and reserving 20% for model verification. The results demonstrated an 85% accuracy on the training set and an 87% accuracy on the testing set. Additionally, two drought events occurring during the winter wheat growing period in Henan Province were monitored, and the validity of these droughts was confirmed using on-site soil moisture and the vegetation supply water index (VSWI). The findings indicated a high incidence of agricultural drought in the southwestern part of Henan Province, while the central and northern regions experienced a lower incidence during the jointing to heading and filling stages. Subsequently, leveraging the results from the random forest drought monitoring, this study conducted a time series analysis using the Mann–Kendall test and a spatial analysis employing Moran’s I index to examine the temporal and spatial distribution of agricultural drought in Henan Province. This analysis aimed to unveil trends in soil moisture changes affecting agricultural drought, as observed via the SMAP satellite (NASA). The results suggested a possible significant spatial auto-correlation in the occurrence of agricultural drought.
Effects of Anaerobic Soil Disinfestation for Soilborne Disease and Weed Management on Baby Leaf Lettuce Performance in a High Tunnel Organic Production System
Authors: Vincent Isaac R. , Rosskopf Erin N. , Brecht Jeffrey K. , Dufault Nicholas S. , Sandoya-Miranda Germán , Zhao Xin
Published: April 2024

The use of high tunnels, which allows growers to extend their season and improve yields, is increasing in the Southeastern U.S., yet growers face challenges related to weed and disease management, particularly in organic systems. On-station experiments were conducted during fall 2021 and spring 2022 in a split-plot design to assess the efficacy of anaerobic soil disinfestation (ASD) in high tunnels for the organic production of direct-seeded baby leaf lettuce. Soil treatments (ASD, Compost, and Control) and lettuce types (romaine and oakleaf cultivars) were included in whole plots and subplots, respectively. The ASD-treated soils received molasses and granular organic fertilizer as carbon and nitrogen sources. The Compost treatment involved the application of yard waste-based compost and the same organic fertilizer, while the Control soils received organic fertilizer only. The ASD treatment period lasted 8 days rather than the typical 21-day period evaluated in Florida. Crop yield, biometrics (leafy dry matter content, specific leaf area, specific leaf weight), and lettuce quality attributes (leaf color, soluble solids content, total titratable acidity, ferric reducing antioxidant power, ascorbic acid content, total phenolics) were assessed following harvest. In both trials, numerical differences in lettuce fresh weight yield between soil treatments were evident, though not statistically significant. Differences in leaf quality attributes were driven by lettuce cultivar rather than soil treatments. Bottom rot incidence caused by Rhizoctonia solani was reduced by 93% and 87% in the ASD-treated plots compared with the Compost- and Control-treated soils during the spring 2022 trial. The ASD-treated soils had a reduced population density of broadleaf weeds in both baby leaf lettuce production trials. The current study presents novel evidence of the potential of integrating ASD into HT organic production systems without reducing the yield and quality attributes of direct-seeded baby leafy greens compared with common grower practices.
Organic Fertilizer with High Nutrient Levels Affected Peanut-Growing Soil Bacteria More Than Fungi at Low Doses
Authors: Zhang Xianying , Li Pengcheng , Zhao Mingyi , Wang Shuqiang , Sun Baoyi , Zhang Yulan , Wang Yonghuan , Chen Zhenhua , Xie Hongtu , Jiang Nan , Li Tingting
Published: April 2024

The breeding of laying hens and broilers in China has increased tremendously. Wet organic fertilizer prepared from hen manure using high-temperature container fermentation preserves high levels of nutrients and a diverse microbial community. We applied low doses of organic fertilizer to peanuts in the black soil area of China’s northeastern region. Based on the calculation of nitrogen content, treatments were set as follows: chemical fertilizer (PCF), organic fertilizer (POF, 4500 kg·ha−1), and replacement of 50% chemical fertilizer with organic fertilizer (PR, 2250 kg·ha−1). Compared to the plots with chemical fertilizers, the use of organic fertilizer and replacing 50% of the chemical fertilizers with organic fertilizer significantly increased peanut yields. Both the organic fertilizer and replacing 50% of the chemical fertilizers with organic fertilizer did not significantly affect the activities of the most tested soil enzymes related to carbon transformation and the absolute abundance of microorganisms. However, they did significantly enhance soil dehydrogenase and α-glucosidase. The community abundance ratio of fungi/bacteria trended downward, leading to soil with a high-fertility bacterial composition. The replacement of 50% chemical fertilizer with organic fertilizer significantly enhanced the species richness and diversity of the bacterial and fungal communities. Organic fertilizer treatment significantly increased the relative abundance of Gemmatimonas and Sphingomonas. The relative abundance of Mycobacterium in the treatment where 50% of the chemical fertilizers were replaced with organic fertilizer was significantly lower than that in the organic fertilizer treatment. PCoA results showed that the low-dose organic fertilizer treatment, replacing 50% of the chemical fertilizers with organic fertilizer, had a significant impact on the composition of soil bacterial communities.
The Impact of Magnesium on the Growth, Physiology and Quality of Tea (Camellia sinensis L.) Plants under Acid Stress
Authors: Lv Xinhang , Kong Haimin , Luo Yubo , Dong Dubin , Liu Wenbin , Wu Dongtao , Ye Zhengqian , Ma Jiawei , Liu Dan
Published: April 2024

Magnesium plays a crucial role in plant physiological processes. However, the specific mechanisms underlying the response of tea plants to altered magnesium nutrition under acid stress remain unclear. This study investigates how root environment acidification impacts tea seedlings and the role of magnesium (Mg) in mitigating these effects. We examine varying pH and Mg levels’ influence on tea seedlings’ resistance to abiotic stress, focusing on antioxidant capacity and nutritional content. In a hydroponic experiment, we varied root pH (3.5, 5.0, 6.5) and Mg concentrations (0.01, 0.4, 0.8 mM), assessing parameters like antioxidant capacity, peroxidative damage, and nutritional content at 1, 7, 15, and 30 days post treatment. Root environment acidification and Mg deficiency worsened peroxidative damage in tea plant leaves and roots. Increased Mg supplementation enhanced antioxidant enzyme activity, reducing malondialdehyde and mitigating oxidative damage from root environment acid stress. Under acid stress, 0.8 mM Mg significantly increased tea leaf polyphenols, amino acids, and water-soluble extracts. Mg notably boosted chlorophyll content, surpassing lower Mg levels at pH 5. Additionally, Mg reversed root vitality inhibition induced by acid stress, leading to increased nitrogen, potassium, and Mg concentrations in leaves, promoting balanced nutrient absorption. Mg supplementation is crucial for enhancing tea plant antioxidant capacity, alleviating growth inhibition from root-environment acid stress, and improving chlorophyll content and root vitality, highlighting Mg’s significance in tea cultivation and broader agricultural practices.
Physiological and Antioxidative Effects of Strontium Oxide Nanoparticles on Wheat
Authors: Kaysım Mustafa Güven , Kumlay Ahmet Metin , Haliloglu Kamil , Türkoğlu Aras , Piekutowska Magdalena , Nadaroğlu Hayrunnisa , Alayli Azize , Niedbała Gniewko
Published: April 2024

We explored the impact of strontium oxide nanoparticles (SrO-NPs), synthesized through a green method, on seedling growth of bread wheat in hydroponic systems. The wheat plants were exposed to SrO-NPs concentrations ranging from 0.5 mM to 8.0 mM. Various parameters, including shoot length (cm), shoot fresh weight (g), root number, root length (cm), root fresh weight (g), chlorophyll value (SPAD), cell membrane damage (%), hydrogen peroxide (H2O2) value (µmol/g), malondialdehyde (MDA) value (ng/µL), and enzymatic activities like ascorbate peroxidase (APX) activity (EU/g FW), peroxidase (POD) activity (EU/g FW), and superoxide dismutase (SOD) activity (U/g FW), were measured to assess the effects of SrO-NPs on the wheat plants in hydroponic conditions. The results showed that the SrO-NPs in different concentrations were significantly affected considering all traits. The highest values were obtained from the shoot length (20.77 cm; 0.5 mM), shoot fresh weight (0.184 g; 1 mM), root number (5.39; 8 mM), root length (19.69 cm; 0 mM), root fresh weight (0.142 g; 1 mM), SPAD (33.20; 4 mM), cell membrane damage (58.86%; 4 mM), H2O2 (829.95 µmol/g; 6 mM), MDA (0.66 ng/µl; 8 mM), APX (3.83 U/g FW; 6 mM), POD (70.27 U/g FW; 1.50 mM), and SOD (60.77 U/g FW; 8 mM). The data unequivocally supports the effectiveness of SrO-NPs application in promoting shoot and root development, chlorophyll levels, cellular tolerance, and the activation of enzymes in wheat plants.
A Combined Approach for the Characterization of Small Ruminant Lentivirus Strains Circulating in the Islands and Mainland of Greece
Authors: Bouzalas Ilias , Apostolidi Evangelia D. , Scalas Daniela , Davidopoulou Evangelia , Chassalevris Taxiarchis , Rosati Sergio , Colitti Barbara
Published: April 2024

Small ruminant lentiviruses are a group of viruses infecting goat and sheep worldwide. These viruses exhibit an extraordinary degree of genetic and antigenic variability that severely influence in vivo and in vitro features, as well as diagnostic test results. Small ruminant farming is the most important animal farming business in Greece, with a high impact on the Greek primary economy. Although SRLV infection and its impact on animal production are well established in the country, little is known about the circulating SRLV strains and their prevalence. The aim of this study was to characterize SRLVs circulating in Greece with a combined serological and molecular approach, using the bulk milk matrix collected from 60 farms in different municipalities. This study allowed us to estimate a seroprevalence of around 52% at the herd level. The B1, B2 and A3 subtypes and a novel A viral cluster were identified. Moreover, the amplicon sequencing method allowed us to identify more than one viral subtype in a sample. These results again confirm the high variability of these viruses and highlight the importance of the constant monitoring of viral evolution, in particular in antigens of diagnostic interest.
The Effects of Poria cocos Polysaccharides on Growth Performance, Immunity, and Cecal Microflora Composition of Weaned Piglets
Authors: Zhang Jinzhou , Wang Heming , Meng Shuaitao , Zhang Chuankuan , Guo Liping , Miao Zhiguo
Published: April 2024

This paper aims to identify Poria cocos polysaccharides (PCPs) as a potential feed additive used for swine production; thus, we explored the effects of different dietary inclusion levels of PCP on growth performance, immunity, and cecal microflora composition in weaned piglets. For this, a total of 120 28-day-old Duroc × Landrace × Yorkshire weaned piglets (8.51 ± 0.19 kg; 28 ± 1 days of age) were randomly allocated to five groups that were fed a basal diet supplemented with 0, 0.025%, 0.05%, 0.1%, and 0.2% PCP, respectively, for 42 days. The results indicated that the average daily gain (ADG) and gain/feed ratio were higher in the PCP treatment groups than in the control group, with a linear effect. The serum concentrations of IgG, IgA, IL-2, IFN-γ, the number of CD4+ T cells, and the CD4+-to-CD8+ T-cell ratio (CD4+/CD8+) were increased, while the levels of IL-6 and TNF-α were decreased in the PCP supplementation groups compared with those in the control group. Furthermore, the cytokine mRNA expression levels exhibited a similar trend in the spleen. PCP supplementation also reduced the abundance of Escherichia coli and Salmonella and enhanced that of Lactobacilli and Bifidobacteria in the cecum. In summary, dietary PCP inclusion exerted positive effects on the growth performance, immunity, and cecal microbiota of piglets and showed potential for use as a feed additive for improving the health of weaned piglets, with 0.1% being the optimal dosage.
Range-Wide Phylogeography and Ecological Niche Modeling Provide Insights into the Evolutionary History of the Mongolian Racerunner (Eremias argus) in Northeast Asia
Authors: Tian Lili , Xu Rui , Chen Dali , Ananjeva Natalia B. , Brown Rafe M. , Min Mi-Sook , Cai Bo , Mijidsuren Byambasuren , Zhang Bin , Guo Xianguang
Published: April 2024

The Mongolian racerunner, Eremias argus, is a small lizard endemic to Northeast Asia that can serve as an excellent model for investigating how geography and past climate change have jointly influenced the evolution of biodiversity in this region. To elucidate the processes underlying its diversification and demography, we reconstructed the range-wide phylogeographic pattern and evolutionary trajectory, using phylogenetic, population genetic, landscape genetic, Bayesian phylogeographic reconstruction and ecological niche modeling approaches. Phylogenetic analyses of the mtDNA cyt b gene revealed eight lineages that were unbounded by geographic region. The genetic structure of E. argus was mainly determined by geographic distance. Divergence dating indicated that E. argus and E. brenchleyi diverged during the Mid-Pliocene Warm Period. E. argus was estimated to have coalesced at~0.4351 Ma (Marine Isotope Stage 19). Bayesian phylogeographic diffusion analysis revealed out-of-Inner Mongolia and rapid colonization events from the end of the Last Interglacial to the Last Glacial Maximum, which is consistent with the expanded suitable range of the Last Glacial Maximum. Pre-Last Glacial Maximum growth of population is presented for most lineages of E. argus. The Glacial Maximum contraction model and the previous multiple glacial refugia hypotheses are rejected. This may be due to an increase in the amount of climatically favorable habitats in Northeast Asia. Furthermore, E. argus barbouri most likely represents an invalid taxon. The present study is the first to report a range-wide phylogeography of reptiles over such a large region in Northeast Asia. Our results make a significant contribution towards understanding the biogeography of the entire Northeast Asia.
Effects of Mixed Pasture Legume Phytoestrogens on Superovulatory Response and Embryo Quality in Angus Cows
Authors: Wyse Jessica M. , Nevard Rory P. , Loy Jaymie , Weston Paul A. , Gurusinghe Saliya , McCormick Jeffrey , Weston Leslie A. , Stephen Cyril P.
Published: April 2024

Ovulation and artificial insemination rates have been observed to decrease in sheep and cows when exposed to dietary phytoestrogens at concentrations greater than 25 mg/kg DM. A grazing trial was undertaken to investigate the effects of coumestrol and other key phytoestrogens on the superovulatory response, embryo numbers and quality in beef cows grazing legume pastures. A 7-week controlled grazing trial was conducted with legume and ryegrass pasture treatments, with cows exposed to legumes at two timed treatments, 4 and 7 weeks. Twenty Angus cows were subjected to a conventional estrus synchronization and superovulation protocol. Embryos were recovered via conventional uterine body flushing 7 days post artificial insemination (AI). Numerous phytoestrogens were identified in both pasture and plasma samples, including coumestrol and formononetin. Concentrations of phytoestrogens in the pasture ranged from 0.001 to 47.5 mg/kg DM and 0 to 2.6 ng/mL in plasma. Approximately 50% of cows produced viable embryos 7 days post AI. A significant interaction between the effect of treatment groups on the embryo stage was observed (p < 0.05). The results suggest that concentrations of >25 mg/kg DM of phytoestrogens less than 20 days preceding AI may negatively affect oocyte developmental competence, reduce progesterone production and thus contribute to early embryonic loss.
The Nutritional Value of Biowaste Bovine Slaughterhouse Meals for Monogastric Species Feeding: The Guinea Pig as an Animal Model
Authors: Chirinos-Peinado Doris , Castro-Bedriñana Jorge , Álvaro-Ordoñez Patricia , Quispe-Ramos Rolando , García-Olarte Edgar , Ríos-Ríos Elva
Published: April 2024

Biowaste from slaughterhouses can be recovered to benefit food security and reduce contamination potential. More than 3 billion heads of livestock are consumed worldwide, which will increase by 17% by 2028, generating more biowaste, increasing infectious agents, and causing economic losses due to circular economy principles not being applied. This work evaluated the nutritional quality of four types of biowaste from bovine slaughter which were transformed into a meal for guinea pigs (rumen content (RCM), ears (EaM), blood (BM), and cheeks (CM)) according to their chemical composition, digestible components, energy contribution, and voluntary consumption. For the animal model, adult male guinea pigs were arranged in metabolic cages for feces collection without urinary contamination. Nine guinea pigs were used in each digestibility test. First, a direct digestibility test was conducted using a meal of barley as a reference diet (RD), the indigestibility coefficient of which allowed for the estimation of the digestibility of biowaste meals through indirect calculations; for this, diets composed of 80% of the RD and 20% of the corresponding biowaste meals were evaluated. The difference method was suitable for determining the digestibility of beef biowaste using the indigestibility coefficients of the reference diet to calculate the digestibility of ingredients which could not be offered as 100% of the meal but were incorporated as 20%. The digestible protein and metabolizable energy contents of RCM, EaM, BM, and CM were 10.2% and 2853 kcal/kg, 44.5% and 3325 kcal/kg, 70.7% and 2583 kcal/kg, and 80.8% and 3386 kcal/kg, respectively. The CM and BM feeds had the highest contributions of digestible protein due to their higher nitrogen content, and the CM and EaM feeds had the highest ME contents due to their higher fat contents. The biowaste meal consumption in descending order was CM > RCM > EaM > BM, which were consumed without problems. These results are indicative that these components can be part of guinea pigs’ diets, and it is recommended to continue studies into guinea pig growth and fattening diets with different levels of these biowaste meals.
A Comprehensive Review of Bovine Colostrum Components and Selected Aspects Regarding Their Impact on Neonatal Calf Physiology
Authors: Silva Flávio G. , Silva Severiano R. , Pereira Alfredo M. F. , Cerqueira Joaquim Lima , Conceição Cristina
Published: April 2024

Colostrum contains macro- and micronutrients necessary to meet the nutritional and energy requirements of the neonatal calf, bioactive components that intervene in several physiological aspects, and cells and microorganisms that modulate the calf’s immune system and gut microbiome. Colostrum is sometimes mistaken as transition milk, which, although more nutritive than whole milk, has a distinct biochemical composition. Furthermore, most research about colostrum quality and colostrum management focuses on the transfer of maternal IgG to the newborn calf. The remaining components of colostrum and transition milk have not received the same attention, despite their importance to the newborn animal. In this narrative review, a large body of literature on the components of bovine colostrum was reviewed. The variability of these components was summarized, emphasizing specific components that warrant deeper exploration. In addition, the effects of each component present in colostrum and transition milk on several key physiological aspects of the newborn calf are discussed.
Correlation between Cross-Sectional Anatomy and Computed Tomography of the Normal Six-Banded Armadillo (Euphractus sexcintus) Nasal Cavity and Paranasal Sinuses
Authors: Jaber José , Morales Bordon Daniel , Arencibia Alberto , Corbera Juan , Conde-Felipe Magnolia , Ayala Maria , Encinoso Mario
Published: April 2024

This research aimed to study the rostral part of the head of the six-banded armadillo, applying advanced imaging techniques such as CT. Furthermore, by combining the images obtained through this technique with anatomical cross-sections, an adequate description of the structures that constitute the rostral part of the head of this species is presented. This anatomical information could provide a valuable diagnostic tool for the clinical evaluation of different disorders in the six-banded armadillo’s nasal cavity and paranasal sinuses.
What Can Laboratory Animal Facility Managers Do to Improve the Welfare of Laboratory Animals and Laboratory Animal Facility Staff? A German Perspective
Authors: von der Beck Birte , Wissmann Andreas , Tolba Rene H. , Dammann Philip , Hilken Gero
Published: April 2024

Laboratory animal facility managers must ensure that animal experiments can be carried out under optimal scientific conditions, that all legal requirements are met, and that animal welfare is maximized. Animal experimentation is stressful not only for the animals involved but also for the people who maintain these animals or carry out the experiments. Many of those involved find themselves in a constant conflict between scientific necessity, care, and harm. Under the term Culture of Care, procedures have been developed to reduce the burden of animal experimentation on the animals and the staff involved. The focus here is on what laboratory animal facility managers can do to improve the welfare of laboratory animals and the people working with them. Exemplary measures are the improvement of the housing conditions of laboratory animals, the introduction of uniform handling measures, clear and transparent structures via a quality management system, implementation of a no-blame culture of error (e.g., via Critical Incident Reporting System in Laboratory Animal Science [CIRS-LAS]), and open and respectful communication with all parties involved in animal experimentation, including the public and representatives of the authorities (public webpage, open house policy). The 6 Rs must be considered at all times: replacement, reduction, refinement, respect, responsibility, and reproducibility. We are writing this article from the perspective of laboratory animal facility managers in Germany.
Using Subcritical Water to Obtain Polyphenol-Rich Extracts with Antimicrobial Properties
Authors: Žagar Tjaša , Frlan Rok , Kočevar Glavač Nina
Published: April 2024

The use of green extraction methods that meet the criteria of sustainable and environmentally friendly technologies has been increasing in recent decades due to their many benefits. In this respect, extracts obtained using subcritical water are also gaining increased attention because of their potential antioxidant and antimicrobial properties. Their antimicrobial activity is mainly due to the presence of various polyphenolic compounds. Although the exact mechanism of the antibacterial action of polyphenolic compounds has not yet been fully investigated and described, polyphenols are known to affect the bacterial cell at several cellular levels; among other things, they cause changes and ruptures in the cell membranes of the bacterial cell, affect the inactivation of bacterial enzymes and damage bacterial DNA. The difference in the strength of the antimicrobial activity of the extracts is most likely a result of differences in their lipophilicity and in the number and position of hydroxyl groups and double bonds in the chemical structure of polyphenols. By changing the extraction conditions, especially the temperature, during subcritical water extraction, we affect the solubility of the compounds we want to extract. In general, as the temperature increases, the solubility of polyphenolic compounds also increases, and the reduction of the surface tension of subcritical water at higher temperatures also enables faster dissolution of polyphenolic compounds. Different bacterial strains have different sensitivity to different extracts. However, extracts obtained with subcritical water extraction demonstrate strong antimicrobial activity compared to extracts obtained with conventional methods.
Development of a High-Throughput Analytical Method for Antimicrobials in Wastewater Using an Automated Pipetting and Solid-Phase Extraction System
Authors: Azuma Takashi , Matsunaga Nobuaki , Ohmagari Norio , Kuroda Makoto
Published: April 2024

Antimicrobial resistance (AMR) has emerged and spread globally. Recent studies have also reported the presence of antimicrobials in a wide variety of aquatic environments. Conducting a nationwide monitoring survey of AMR in the environment to elucidate its status and to assess its impact on ecosystems and human health is of social importance. In this study, we developed a novel high-throughput analysis (HTA) system based on a 96-well plate solid-phase extraction (SPE), using automated pipetting and an SPE pre-treatment system. The effectiveness of the system as an HTA for antimicrobials in environmental water was verified by comparing it with a conventional manual analytical system in a domestic hospital over a period of two years and four months. The results of the manual analysis and HTA using a combination of automated pipetting and SPE systems were generally consistent, and no statistically significant difference was observed (p > 0.05) between the two systems. The agreement ratios between the measured concentrations based on the conventional and HTA methods were positively correlated with a correlation coefficient of r = 0.99. These results indicate that HTA, which combines automated pipetting and an SPE pre-treatment system for rapid, high-volume analysis, can be used as an effective approach for understanding the environmental contamination of antimicrobials at multiple sites. To the best of our knowledge, this is the first report to present the accuracy and agreement between concentrations based on a manual analysis and those measured using HTA in hospital wastewater. These findings contribute to a comprehensive understanding of antimicrobials in aquatic environments and assess the ecological and human health risks associated with antimicrobials and antimicrobial-resistant bacteria to maintain the safety of aquatic environments.
Revisiting the Checkerboard to Inform Development of β-Lactam/β-Lactamase Inhibitor Combinations
Authors: Bentley Darren J.
Published: April 2024

A two-dimensional “checkerboard” array employing systematic titration (e.g., serial two-fold dilutions) is a well-established in vitro method for exploring the antibacterial effects of novel drug combinations. Minimum inhibitory concentrations (MICs) on the checkerboard are isoeffective points at which the antibiotic potency is the same. Representations of checkerboard MIC curves for a β-lactam and β-lactamase inhibitor combination are used in hypothetical “thought experiments” and reveal the ways in which current practices can be improved. Because different types of response (i.e., independence vs. additivity vs. one effective agent; interaction vs. noninteraction) produce different MIC curves, data from different strains/isolates should not be pooled indiscriminately, as the composition of a pooled dataset will influence any derived pharmacokinetic/pharmacodynamic (PK/PD) index. Because the β-lactamase inhibitor threshold concentration (CT) parameter is a function of the β-lactam partner dosing regimen, it is not possible to derive a universal PK/PD index target based on CT. Alternative susceptibility testing methods represent different planes through the checkerboard; a fixed ratio method is less prone to bias for all β-lactam and β-lactamase inhibitor combinations. Susceptibility test MICs will often not reflect the sensitivity of the strain/isolate to the β-lactamase inhibitor, so the use of these MICs to normalize PK/PD indices is inappropriate.