bluuebunny/crossref_metadata_2025 huggingface dataset:
Characteristics of Terrestrial Heat Flow and Lithospheric Thermal Structure in Typical Intermountain Basin at the Eastern Foot of Yanshan Mountain, North China
Authors: Liu Feng , Wang Andong , Wang Guiling , Zhang Wei , Liao Yuzhong , Tong Jue
Published: November 2021

The Yanshan area is rich in geothermal resources. However, limited research has been done on the geothermal resources of intermountain basins in the area, which restrict the exploration and development of geothermal resources. In this study, the Yanheying Basin, a typical intermountain basin located in the eastern foothills of Yanshan Mountain, is selected to perform a comprehensive analysis regarding heat flow and lithospheric thermal structure, to have a better understanding of the geothermal background and resource utilization potential of the area. The methods of deep borehole temperature logging, and rock sampling and testing were applied. With geological and geophysical data collected in the surrounding basins, the thermophysical parameters of rock formations, terrestrial heat flow and temperature distribution on a crustal-scale around the basin were analyzed. Results show that the ratio of crust heat flow to that of the mantle in the Yanheying Basin is 0.68, which falls in the range of that of the North China Plain (0.6–0.8), showing an obvious result of lithospheric thinning. The results also show that both crust and mantle heat flows in the Yanheying Basin are far lower than those of the North China Plain. it indicates that the crust of the Yanheying Basin and the entire Yanshan area has experienced a low degree of damage. That provides a piece of good thermodynamic evidence for the spatial variation of North China Craton destruction. The depth of the Curie surface in the study area is estimated to be about 24 km, which is consistent with the results of aeromagnetic data analysis. It confirms the results of lithospheric thermal structure and deep temperature distribution in the Yanheying Basin. The Yanheying Basin has certain potential for geothermal resources utilization in the depth of 2–6 km, where probably exist several carbonate thermal reservoirs with medium-temperature geothermal water. The above results can provide new insights into the geothermal research and exploration of intermountain basins in the Yanshan area.
Trends in Western North Pacific Tropical Cyclone Intensity Change Before Landfall
Authors: Liu Qingyuan , Song Jinjie , Klotzbach Philip J.
Published: November 2021

This study investigates the long-term trend in the average 24-h intensity change (ΔV24) of western North Pacific (WNP) tropical cyclones (TCs) before landfall during June-November for the period from 1970–2019. We find a significant increasing trend in basin-averaged ΔV24 during 1970–2019. The increase in ΔV24 is significant over the northern South China Sea (17.5°-25°N, 107.5°-120°E) and to the east of the Philippines (7.5°-15°N, 122.5°-132.5°E), implying a slower weakening rate before landfall for the South China Sea and an increased intensification rate before landfall for the region east of the Philippines. We find a significant linkage between changes in ΔV24 and several large-scale environmental conditions. The increased ΔV24 before landfall in the above two regions is induced by a warmer ocean (e.g., higher sea surface temperatures, maximum potential intensity and TC heat potential) and greater upper-level divergence, with a moister mid-level atmosphere also aiding the ΔV24 increase east of the Philippines. Our study highlights an increasing tendency of ΔV24 before landfall, consistent with trends in ΔV24 over water and over land as found in previous publications.
Biogas Upgradation Through CO2 Conversion Into Acetic Acid via Microbial Electrosynthesis
Authors: Roy Moumita , Yadav Sukrampal , Patil Sunil A.
Published: November 2021

Biogas is one of the promising futuristic renewable energy sources with enormous market potential. However, the presence of CO2 lowers down the calorific value of biogas. Hence, various biogas upgradation technologies are under intense investigation to increase the methane content to the desired level. This study reports on enhancing methane content in biogas through CO2 sequestration into acetic acid via microbial electrosynthesis (MES) process. The previously enriched mixed chemolithoautotrophic microbial culture dominated by Acetobacterium spp. used CO2 present in the biogas as the sole carbon source. After establishing a stable performing biocathode at a fixed cathodic potential of −1 V (vs. Ag/AgCl) through batch mode operation, biogas was fed continuously at different feed rates, viz., 0.5, 0.3, and 0.2 ml/min to the cathode chamber. The highest feed rate of 0.5 ml/min was least effective both for methane content increment (from 61 ± 3% to 86 ± 2%) and acetic acid titer (1.5 ± 0.5 g/L; 0.107 ± 0.02 g/L/d.). In comparison, the lowest flow rate of 0.2 ml/min was the most effective for the intended process (methane upgradation from 62 ± 7% to 93 ± 3% and acetic acid titer 3.4 ± 0.6 g/L produced at 0.24 ± 0.04 g/L/d rate). Both acetic acid bioproduction and biogas upgradation occurred best at an Ecell of 3.3 ± 0.35 V at the low feed rate. A maximum of 84 ± 7%, 57 ± 10% and 29 ± 2% coulombic, carbon and energetic efficiencies, respectively, were achieved in acetic acid. Cyclic voltammograms of biocathodes revealed the decrease in hydrogen evolution potential and increased bioelectrocatalysis, thereby suggesting the contribution of microbes in the process. Acetobacterium, which is known for CO2 fixation, was found to be the dominant microbial genus in biogas fed reactors. The demonstrated approach not only offers the advantage of obtaining two products, one in the bulk phase and the other in the off-gas, it also validates the applicability of the bioelectrochemical biogas upgradation technology.
Assessment of the Ecological Status of Rongjiang Estuary (China) Under Human Pressure, Using Biotic Indices Based on Benthic Macroinvertebrates
Authors: Li Yafang , Wang Lianggen , Ning Jiajia , Xu Lei , Huang Delian , Liu Shuangshuang , Du Feiyan
Published: November 2021

Rongjiang River, the second largest river system in Guangdong Province, flows through the main urban areas of Jieyang and Shantou cities before reaching the South China Sea. Human activities in the surrounding area pose significant threats to this aquatic ecosystem. The ecological status (ES) of the benthic ecosystem of the Rongjiang River estuary has not yet been conducted using indices based on the macrobenthic fauna, which is important for evaluating environmental health. Here, we used four biotic indices (the AZTI’s Marine Biotic Index (AMBI) and Multivariate AZTI’s Marine Biotic Index (M-AMBI), and taxonomic distinctness indices (average taxonomic distinctness Δ+ and variations in taxonomic distinctness Λ+) to appraise the current ES of benthic communities in Rongjiang estuary. Samples were taken from 11 sampling sites located in six general regions: western aquaculture zones, aquaculture zones, Hanjiang River water channel, Shantou City, Shantou Port, and near the ocean. The benthic ecosystem of this estuary is greatly disturbed: the ES of the aquaculture zones and the sites near the Hanjiang River water channel, Shantou City, and close to the ocean was poorer compared with that of other areas; ES was also poorer in winter than in summer. Generalized linear models revealed that Shannon-Wiener index was negatively correlated with dissolved inorganic nitrogen (p < 0.01), M-AMBI was negatively correlated with temperature and dissolved inorganic nitrogen (p < 0.05 and p < 0.01, respectively), and Λ+ was negatively correlated with pH (p < 0.05). The AMBI, M-AMBI, Δ+, and Λ+ indices were suitable for assessing the ES of the benthic ecosystem in an anthropogenically disturbed estuary.
Heritability Enrichment of Immunoglobulin G N-Glycosylation in Specific Tissues
Authors: Li Xingang , Wang Hao , Zhu Yahong , Cao Weijie , Song Manshu , Wang Youxin , Hou Haifeng , Lang Minglin , Guo Xiuhua , Tan Xuerui , Han Jingdong J. , Wang Wei
Published: November 2021

Genome-wide association studies (GWAS) have identified over 60 genetic loci associated with immunoglobulin G (IgG) N-glycosylation; however, the causal genes and their abundance in relevant tissues are uncertain. Leveraging data from GWAS summary statistics for 8,090 Europeans, and large-scale expression quantitative trait loci (eQTL) data from the genotype-tissue expression of 53 types of tissues (GTEx v7), we derived a linkage disequilibrium score for the specific expression of genes (LDSC-SEG) and conducted a transcriptome-wide association study (TWAS). We identified 55 gene associations whose predicted levels of expression were significantly associated with IgG N-glycosylation in 14 tissues. Three working scenarios, i.e., tissue-specific, pleiotropic, and coassociated, were observed for candidate genetic predisposition affecting IgG N-glycosylation traits. Furthermore, pathway enrichment showed several IgG N-glycosylation-related pathways, such as asparagine N-linked glycosylation, N-glycan biosynthesis and transport to the Golgi and subsequent modification. Through phenome-wide association studies (PheWAS), most genetic variants underlying TWAS hits were found to be correlated with health measures (height, waist-hip ratio, systolic blood pressure) and diseases, such as systemic lupus erythematosus, inflammatory bowel disease, and Parkinson’s disease, which are related to IgG N-glycosylation. Our study provides an atlas of genetic regulatory loci and their target genes within functionally relevant tissues, for further studies on the mechanisms of IgG N-glycosylation and its related diseases.
Strength Prediction of Coal-Based Solid Waste Filler Based on BP Neural Network
Authors: Feng Feisheng , Li Lirong , Zhang Jiqiang , Yang Zhen , Chi Xiaolou
Published: November 2021

The compressive strength of filling body is an important index to characterize the filling and mining effect of coal mine. In order to accurately predict the strength of coal-based solid waste filler (CBSWF) to guide the safe, efficient, and green mining of coal mine, coal gangue is used as coarse material; fly ash, desulfurization gypsum, gasification slag, and furnace bottom slag are used as fine materials; and cement is used as gelling agent. The compressive strength and bleeding rate of CBSWF are tested through orthogonal test, and the strength of CBSWF at different curing ages is predicted by using a 4-11-3 three-layer BP neural network structure. The results show that the correlation coefficient r of strength prediction of CBSWF is 0.99987, which can accurately predict the strength of CBSWF. Orthogonal test combined with the BP neural network can reduce the number of tests without losing generality, make full use of the advantages of adaptive nonlinear optimization of the BP neural network, and improve the operation efficiency of the model, fast prediction speed, and high accuracy.
Interspecific Recombination Between Zucchini Tigre Mosaic Virus and Papaya Ringspot Virus Infecting Cucurbits in China
Authors: Peng Bin , Liu Liming , Wu Huijie , Kang Baoshan , Fei Zhangjun , Gu Qinsheng
Published: November 2021

Recombination drives evolution of single-stranded RNA viruses and contributes to virus adaptation to new hosts and environmental conditions. Intraspecific recombinants are common in potyviruses, the largest family of single-stranded RNA viruses, whereas interspecific recombinants are rare. Here, we report an interspecific recombination event between papaya ringspot potyvirus (PRSV) and zucchini tigre mosaic potyvirus (ZTMV), two potyviruses infecting cucurbit crops and sharing similar biological characteristics and close phylogenetic relationship. The PRSV-ZTMV recombinants were detected through small RNA sequencing of viruses infecting cucurbit samples from Guangxi and Henan provinces of China. The complete nucleotide (nt) sequences of the interspecific recombinant viruses were determined using overlapping RT-PCR. Multiple sequence alignment, recombination detection analysis and phylogenetic analysis confirmed the interspecific recombination event, and revealed an additional intraspecific recombination event among ZTMV populations in China. The symptoms and host ranges of two interspecific recombinant isolates, KF8 and CX1, were determined through experimental characterization using cDNA infectious clones. Surveys in 2017 and 2018 indicated that the incidences of the interspecific recombinant virus were 16 and 19.4%, respectively, in cucurbits in Kaifeng of Henan province. The identified interspecific recombinant virus between PRSV and ZTMV and the novel recombination pattern with the recombination site in HC-pro in potyvirid provide insights into the prevalence and evolution of ZTMV and PRSV in cucurbits.
Quantification of Epileptogenic Network From Stereo EEG Recordings Using Epileptogenicity Ranking Method
Authors: Parasuram Harilal , Gopinath Siby , Pillai Ashok , Diwakar Shyam , Kumar Anand
Published: November 2021

Introduction: Precise localization of the epileptogenic zone is very essential for the success of epilepsy surgery. Epileptogenicity index (EI) computationally estimates epileptogenicity of brain structures based on the temporal domain parameters and magnitude of ictal discharges. This method works well in cases of mesial temporal lobe epilepsy but it showed reduced accuracy in neocortical epilepsy. To overcome this scenario, in this study, we propose Epileptogenicity Rank (ER), a modified method of EI for quantifying epileptogenicity, that is based on spatio-temporal properties of Stereo EEG (SEEG).

Methods: Energy ratio during ictal discharges, the time of involvement and Euclidean distance between brain structures were used to compute the ER. Retrospectively, we localized the EZ for 33 patients (9 for mesial-temporal lobe epilepsy and 24 for neocortical epilepsy) using post op MRI and Engel 1 surgical outcome at a mean of 40.9 months and then optimized the ER in this group.

Results: Epileptic network estimation based on ER successfully differentiated brain regions involved in the seizure onset from the propagation network. ER was calculated at multiple thresholds leading to an optimum value that differentiated the seizure onset from the propagation network. We observed that ER < 7.1 could localize the EZ in neocortical epilepsy with a sensitivity of 94.6% and specificity of 98.3% and ER < 7.3 in mesial temporal lobe epilepsy with a sensitivity of 95% and specificity of 98%. In non-seizure-free patients, the EZ localization based on ER pointed to brain area beyond the cortical resections.

Significance: Methods like ER can improve the accuracy of EZ localization for brain resection and increase the precision of minimally invasive surgery techniques (radio-frequency or laser ablation) by identifying the epileptic hubs where the lesion is extensive or in nonlesional cases. For inclusivity with other clinical applications, this ER method has to be studied in more patients.
High-Sensitivity RF Choke-Enhanced Dipole Antenna-Coupled Nb5N6 THz Detector
Authors: Jiang Chengtao , Tu Xuecou , Wan Chao , Kang Lin , Jia Xiaoqing , Chen Jian , Wu Peiheng
Published: November 2021

In this study, we demonstrate an Nb5N6 terahertz (THz) detector with radio frequency (RF) choke-enhanced dipole antenna structure for 0.3 THz detection. The maximum electric field intensity of 218 V/m is obtained by optimizing the parameters of the dipole antenna with RF choke. Compared to a dipole antenna without RF choke, the electric field intensity of that with RF choke is improved by 2.6 times. The RF choke-enhanced dipole antenna-coupled Nb5N6 THz detector is fabricated and characterized. The measured maximum responsivity of the detector is 1100 V/W at 0.308 THz, and the corresponding noise equivalent power (NEP) is 6.4 × 10–12 W/Hz1/2. The measured response time of the Nb5N6 THz detector is as low as 8.46 μs. Furthermore, the Nb5N6 THz detector is applied to a homemade THz transmission imaging system for demonstrating its performance. The THz imaging results of a blade and access card show that the contrast of the blade image is sharp and the components hidden within the access card are clearly visible. This indicates that the Nb5N6 THz detector can be used in THz imaging, particularly in THz active imaging, which will have greater application prospects.
The Performance Impact of New Ventures in Working Environment and Innovation Behavior From the Perspective of Personality Psychology
Authors: Yang Shufang , Wu Hainan
Published: November 2021

A new venture barely makes a profit in its initial stage, and its success depends on innovation. Innovation is related to the work environment, and the innovation behavior of employees is of great significance to the performance improvement of new venture. Based on the previous research, in this study, hypotheses on the correlation between work environment, employee innovation behavior, and corporate performance are put forward first. Then, with team cooperation, organizational incentive, leadership support, sufficient resources, and work pressure as the factors of the work environment, the bosses, middle and senior managers involved in entrepreneurship, and the main members of the entrepreneurial team of 202 newly established enterprises in developed regions are surveyed online or in scene. Multivariate hierarchical regression analysis is performed to analyze the data collected from the questionnaire. The results show that the effective recovery rate of the questionnaire is 86.4%; the number of traditional enterprises is 108 (53.47%), and that of R&D enterprises is 68 (33.66%); teamwork, leadership support, and work pressure are all correlated with employees’ innovative behavior (P < 0.05), while organizational motivation and sufficient resources are not correlated with employees’ innovative behavior (P > 0.05); employee innovation behavior is positively correlated with enterprise performance (β = 0.375, P ≤ 0.01); the working environment and employee innovation behavior promote enterprise performance (β = 0.433, P ≤ 0.01); and the working environment affects the relationship between employee innovation behavior and enterprise performance (β = 0.399, P ≤ 0.05). The study theoretically enriches the research on the relationship between innovation behavior, work environment, and enterprise performance of new ventures. In practice, it is suggested that start-up enterprises provide good working environment for employees and attach importance to innovation activities at the individual level of employees, which provides useful guidance and reference for the development of Chinese start-up enterprises.
Cumulative Childhood Adversity and Its Associations With Mental Health in Childhood, Adolescence, and Adulthood in Rural China
Authors: Shen Wensong
Published: November 2021

Capitalizing on a 15-year longitudinal dataset of 9–12 years old children in rural China, this study adopts a life course perspective and analyzes cumulative childhood adversity and its associations with mental health problems from childhood to adulthood. Four domains of childhood life are selected to construct cumulative childhood adversity: socioeconomic hardship, family disruption, physical issue, and academic setback. Overall, cumulative childhood adversity significantly associates with children’s internalizing and externalizing problems as well as adults’ depression and self-esteem. However, cumulative childhood adversity has no significant relationship with internalizing and externalizing problems in adolescence. Furthermore, different domains of childhood adversity matter differently for mental health problems in different life stages. Physical issue and academic setback have the strongest association with internalizing and externalizing problems in childhood, while only socioeconomic hardship has a significant relationship with depression and self-esteem in adulthood. The relationship between cumulative childhood adversity and adult mental health problems is fully mediated by educational attainment. Finally, there is no gender difference in either the occurrence of cumulative childhood adversity or the association between cumulative childhood adversity and mental health problems.
Communication About Chronic Pain in Older Persons' Social Networks: Study Protocol of a Qualitative Approach
Authors: Merminod Gilles , Weber Orest , Vaucher Carla , Semlali Imane , Terrier Anamaria , Decosterd Isabelle , Rubli Truchard Eve , Singy Pascal
Published: November 2021

A lack of social relations appears to impact on health and life expectancy among the older persons. The quality and diversity of social relations are correlated with good health and well-being in later life. Chronic pain is a crucial issue in aging population. Effective communication between the older persons with chronic pain, their relatives and the actors of the healthcare system facilitates the management of this condition. Studies on communication in later life generally do not consider the older persons' social network as a whole, focusing only a specific segment (e.g., family or medical staff). This lack of scientific data prevents the actors of the healthcare system from offering solutions to bridge clinically relevant communication gaps. As a consequence, our study has three objectives: (1) to identify how the older persons perceive communication about chronic pain with their social network; (2) to identify their unmet communication needs; (3) to develop recommendations that improve communication about chronic pain in later life. The study will be divided into two phases. The first phase will meet objectives 1 and 2. It will involve individual interviews with about 50 people over 75 years old suffering from chronic pain and without major cognitive or auditory troubles. In this phase, we will apply a multi-layered analysis. We will map the older persons' personal network and identify their communication practices and needs, by combining content and discourse analysis with social network theories. The second phase of the study will aim at recommendations based on the results of the first phase (objective 3). It will require focus groups with different sets of stakeholders (older persons, relative caregivers, health professionals, decision-makers). In the second phase, we will use content analysis to pinpoint the concerns and suggestions for action. The results will be disseminated on three levels: (1) to the scientific world (specialists in the field of health and aging and health communication); (2) to health practitioners working with older persons; (3) to society at large, with a focus on institutions and groups directly concerned by the issue.
Quantum Prisoner’s Dilemma and High Frequency Trading on the Quantum Cloud
Authors: Khan Faisal Shah , Bao Ning
Published: November 2021

High-frequency trading (HFT) offers an excellent use case and a potential killer application of the commercially available, first generation quasi-quantum computers. To this end, we offer here a simple game-theoretic model of HFT as the famous two player game, Prisoner’s Dilemma. We explore the implementation of HFT as an instance of Prisoner’s Dilemma on the (quasi) quantum cloud using the Eisert, Wilkens, and Lewenstein quantum mediated communication protocol, and how this implementation can not only increase transaction speed but also improve the lot of the players in HFT. Using cooperative game-theoretic reasoning, we also note that in the near future when the internet is properly quantum, players will be able to achieve Pareto-optimality in HFT as an instance of reinforced machine learning.
Strengths and Limitations of Conventional Approaches to the Risk Assessment and Management of EMF Exposure From 5G and B5G Networks
Authors: Mazar Haim , Ball David J.
Published: November 2021

This paper describes the current science-based approach to ensuring public safety from RF-EMF base stations. It acknowledges that a degree of public concern persists despite the fact that dose limits are seldom if ever approached in practice. One plausible contributor to the discord is a tendency within parts of academia and by some planning authorities to pursue a technocratic policy approach to siting wherein public concerns are attributed to scientific uncertainty over health risks, whereas the reality is that societal concerns over base stations is broader. Thus, potential mis-framing of the debate as a purely scientific issue leads to inappropriate risk communication exercises which in fact polarise the situation by disenfranchising other potentially legitimate siting concerns. It is recommended that more attention should be paid to the non-health related siting issues, which could be handled by a thoughtful democratic / deliberative process, and that this should not be conflated with the scientific debate over the uncertain and possibly non-existent risks posed by RF EMF complying with the existing science-based dose limits.
A Semilinear Parameter-Varying Observer Method for Fabric-Reinforced Soft Robots
Authors: Bui Phuc D.H. , Schultz Joshua A.
Published: November 2021

This paper presents an observer architecture that can estimate a set of configuration space variables, their rates of change and contact forces of a fabric-reinforced inflatable soft robot. We discretized the continuum robot into a sequence of discs connected by inextensible threads; this allows great flexibility when describing the robot’s behavior. At first, the system dynamics is described by a linear parameter-varying (LPV) model that includes a set of subsystems, each of which corresponds to a particular range of chamber pressure. A real-world challenge we confront is that the physical robot prototype exhibits a hysteresis loop whose directions depend on whether the chamber is inflating or deflating. In this paper we transform the hysteresis model to a semilinear model to avoid backward-in-time definitions, making it suitable for observer and controller design. The final model describing the soft robot, including the discretized continuum and hysteresis behavior, is called the semilinear parameter-varying (SPV) model. The semilinear parameter-varying observer architecture includes a set of sub-observers corresponding to the subsystems for each chamber pressure range in the SPV model. The proposed observer is evaluated through simulations and experiments. Simulation results show that the observer can estimate the configuration space variables and their rate of change with no steady-state error. In addition, experimental results display fast convergence of generalized contact force estimates and good tracking of the robot’s configuration relative to ground-truth motion capture data.
Recent Development of Ruminant Vaccine Against Viral Diseases
Authors: Choudhury Sk Mohiuddin , Ma XuSheng , Dang Wen , Li YuanYuan , Zheng HaiXue
Published: November 2021

Pathogens of viral origin produce a large variety of infectious diseases in livestock. It is essential to establish the best practices in animal care and an efficient way to stop and prevent infectious diseases that impact animal husbandry. So far, the greatest way to combat the disease is to adopt a vaccine policy. In the fight against infectious diseases, vaccines are very popular. Vaccination's fundamental concept is to utilize particular antigens, either endogenous or exogenous to induce immunity against the antigens or cells. In light of how past emerging and reemerging infectious diseases and pandemics were handled, examining the vaccination methods and technological platforms utilized for the animals may provide some useful insights. New vaccine manufacturing methods have evolved because of developments in technology and medicine and our broad knowledge of immunology, molecular biology, microbiology, and biochemistry, among other basic science disciplines. Genetic engineering, proteomics, and other advanced technologies have aided in implementing novel vaccine theories, resulting in the discovery of new ruminant vaccines and the improvement of existing ones. Subunit vaccines, recombinant vaccines, DNA vaccines, and vectored vaccines are increasingly gaining scientific and public attention as the next generation of vaccines and are being seen as viable replacements to conventional vaccines. The current review looks at the effects and implications of recent ruminant vaccine advances in terms of evolving microbiology, immunology, and molecular biology.
Insight Into an Outbreak of Canine Distemper Virus Infection in Masked Palm Civets in China
Authors: Shi Ning , Zhang Le , Yu Xiuhua , Zhu Xiangyu , Zhang Shu , Zhang Daining , Duan Ming
Published: November 2021

In August 2019, a suspected outbreak of canine distemper was observed in a masked palm civet farm that also received stray civets and rescued wild civets in Henan Province of China. A virulent canine distemper virus (CDV) strain, named HN19, from vaccinated masked palm civets was the etiologic agent identified in this outbreak using RT-PCR and sequencing of the complete genome. Serological analysis indicated a lower positive rate of CDV-neutralizing antibody in wild civets than in captive civets. Phylogenetic analysis of viral hemagglutinin (H) and the complete genome showed high identities with Rockborn-like strains at the nucleotide (98.7~99.72%) and the closest nucleotide similarity with a strain that killed lesser pandas in China in 1997, but low identities with America-1 strains (vaccine strains). Most importantly, one distinct amino acid exchange in the H protein at position 540 Asp → Gly (D540G), which confers CDV with an improved ability to adapt and utilize the human receptor, was observed in HN19. This study represents the first reported outbreak of a Rockborn-like CDV strain infection in masked palm civets in China. Based on this report, the existence of Rockborn-like strains in Chinese wild animals may not only cause immune failure in captive animals, but may also confer increased zoonotic potential.
Cognición, constructivismo y narrativa: en busca de un sentido para las silabas.
Authors: Gonçalves Oscar F.
Published: October 2021

El constructivismo en terapia supone nuevos desafíos para la psicología y para el objeto de su estudio. En este artículo el autor presenta cuatro presupuestos de una versión narrativa del constructivismo: existencia como conocimiento; conocimiento como hermenéutica; hermenéutica como discurso narrativo; y narrativa como cultura. Son discutidos cada uno de ellos, analizadas sus implicaciones para una nueva concepción de la psicología y de sus prácticas terapéuticas.
Vittorio Guidano y el enfoque cognitivo post-racionalista. Notas sobre una entrevista.
Authors: Balbi Juan
Published: October 2021

Vittorio Guidano, terapeuta italiano radicado en Roma, desarrolla desde hace algo más de una década un modelo psicoterapéutico que constituye un verdadero punto de inflexión en la psicología y en la psicoterapia de nuestros días, el Modelo Cognitivo Procesal Sistémico. Este modelo se sustenta en un enfoque epistemológico post-racionalista que incorpora las nuevas nociones de realidad y de conocimiento, que la ciencia física comenzó a construir desde principios de siglo, y que, en los últimos años, han sido sintetizadas por Humberto Maturana a partir del estudio biológico del conocimiento. Esta síntesis a su vez, ha dado origen a nuevos criterios en las nociones del organismo y adaptación.
Development of Optimized Ultrasound-Assisted Extraction Methods for the Recovery of Total Phenolic Compounds and Anthocyanins from Onion Bulbs
Authors: González-de-Peredo Ana V. , Vázquez-Espinosa Mercedes , Espada-Bellido Estrella , Ferreiro-González Marta , Carrera Ceferino , Barbero Gerardo F. , Palma Miguel
Published: November 2021

Allium cepa L. is one of the most abundant vegetable crops worldwide. In addition to its versatile culinary uses, onion also exhibits quite interesting medicinal uses. Bulbs have a high content of bioactive compounds that are beneficial for human health. This study intends to develop and optimize two appropriate ultrasound-assisted methods for the extraction of the phenolic compounds and anthocyanins present in red onion. A response surface methodology was employed and, specifically, a Box–Behnken design, for the optimization of the methods. The optimal conditions for the extraction of the phenolic compounds were the follows: 53% MeOH as solvent, pH 2.6, 60 °C temperature, 30.1% amplitude, 0.43 s cycle, and 0.2:11 g sample/mL solvent ratio. On the other hand, the optimal conditions for the anthocyanins were as follows: 57% MeOH as solvent, pH 2, 60 °C temperature, 90% amplitude, 0.64 s cycle, and 0.2:15 g sample/mL solvent ratio. Both methods presented high repeatability and intermediate precision, as well as short extraction times with good recovery yields. These results illustrate that the use of ultrasound-assisted extraction, when properly optimized, is suitable for the extraction and quantification of the compounds of interest to determine and improve the quality of the raw material and its subproducts for consumers.
Characterization, Cytotoxicity and Anti-Inflammatory Effect Evaluation of Nanocapsules Containing Nicotine
Authors: Landau Albrecht Carolina , Sperling Laura Elena , Iglesias Braghirolli Daikelly , Pranke Patricia
Published: November 2021

(1) Background: Nanotechnology is an emerging field that can be applied in the biomedical area. In this study, Eudragit nanocapsules (NCs) containing nicotine were produced. Nicotine is the main alkaloid found in tobacco and has anti-inflammatory properties. NCs containing nicotine may be used as an adjuvant therapy in the treatment of inflammation in the central nervous system. (2) Methods: Nanocapsules were prepared by the interfacial deposition of the pre-formed polymer method and characterized in terms of zeta potential, diameter, polydispersity index, pH, encapsulation efficiency (EE), stability and sustained release profile. In vitro tests with the PC12 cell line were performed, such as MTT, LIVE/DEAD and ELISA assays, to verify their cytotoxic and anti-inflammatory effects. (3) Results: The nanocapsules presented satisfactory values of the characterization parameters; however, poor encapsulation was obtained for nicotine (8.17% ± 0.47). The in vitro tests showed that the treatment with nanocapsules reduced cell viability, which suggests that the Eudragit or the amount of polymer on top of the cells may be detrimental to them, as the cells were able to survive when treated with bulk nicotine. ELISA showed an increment in the expression of IL-6 and IL-1β, corroborating the hypothesis that NCs were toxic to the cells because of the increase in the levels of these pro-inflammatory cytokines. (4) Conclusions: This study demonstrates that NCs of Eudragit present toxicity. It is therefore necessary to improve NC formulation to obtain better values for the encapsulation efficiency and reduce toxicity of these nanodevices.
A Biomechanical Evaluation of a Novel Airbag Bicycle Helmet Concept for Traumatic Brain Injury Mitigation
Authors: Tse Kwong Ming , Holder Daniel
Published: November 2021

In this study, a novel expandable bicycle helmet, which integrates an airbag system into the conventional helmet design, was proposed to explore the potential synergetic effect of an expandable airbag and a standard commuter-type EPS helmet. The traumatic brain injury mitigation performance of the proposed expandable helmet was evaluated against that of a typical traditional bicycle helmet. A series of dynamic impact simulations on both a helmeted headform and a representative human head with different configurations were carried out in accordance with the widely recognised international bicycle helmet test standards. The impact simulations were initially performed on a ballast headform for validation and benchmarking purposes, while the subsequent ones on a biofidelic human head model were used for assessing any potential intracranial injury. It was found that the proposed expandable helmet performed admirably better when compared to a conventional helmet design—showing improvements in impact energy attenuation, as well as kinematic and biometric injury risk reduction. More importantly, this expandable helmet concept, integrating the airbag system in the conventional design, offers adequate protection to the cyclist in the unlikely case of airbag deployment failure.
Potential Prognostic Biomarkers of OSBPL Family Genes in Patients with Pancreatic Ductal Adenocarcinoma
Authors: Chou Cheng-Wei , Hsieh Yu-Hsiu , Ku Su-Chi , Shen Wan-Jou , Anuraga Gangga , Khoa Ta Hoang Dang Khoa , Lee Kuen-Haur , Lee Yu-Cheng , Lin Cheng-Hsien , Wang Chih-Yang , Wang Wei-Jan
Published: November 2021

Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal malignancy with poor survival outcomes. In addition, oxysterol-binding protein-like (OSBPL) family members are reported to be involved in lipid binding and transport and play critical roles in tumorigenesis. However, relationships between PDAC and OSBPL family members have not comprehensively been elucidated. In this study, we used the Oncomine and GEPIA 2 databases to analyze OSBPL transcription expressions in PDAC. The Kaplan–Meier plotter and TIMER 2.0 were used to assess the relationships between overall survival (OS) and immune-infiltration with OSBPL family members. Co-expression data from cBioPortal were downloaded to assess the correlated pathways with OSBPL gene family members using DAVID. The expressions of OSBPL3, OSBPL8, OSBPL10, and OSBPL11 were found to be highly upregulated in PDAC. Low expressions of OSBPL3, OSBPL8, and OSBPL10 indicated longer OS. The functions of OSBPL family members were mainly associated with several potential signaling pathways in cancer cells, including ATP binding, integrin binding, receptor binding, and the renin-angiotensin system (RAS) signaling pathway. The transcription levels of OSBPL gene family members were connected with several immune infiltrates. Collectively, OSBPL family members are influential biomarkers for the early diagnosis of PDAC and have prognostic value, with the promise of precise treatment of PDAC in the future.
Regulation of Butyrate-Induced Resistance through AMPK Signaling Pathway in Human Colon Cancer Cells
Authors: Yoo Hee Young , Park So Yeon , Chang Sun-Young , Kim So Hee
Published: November 2021

Butyrates inhibit cell growth in colon cancer cells by inhibiting histone deacetylases. However, chronic exposure to butyrates induces butyrate resistance in colon cancer cells. The mechanism underlying the acquisition of resistance is not yet fully understood. Here, butyrate-resistant (BR) colon cancer cells were developed in HCT116, HT29, and SW480 human colon cancer cells and were confirmed by the increase in the inhibitory concentrations of cell growth by 50% (IC50) compared to their respective parental (PT) cells. Chronic exposure to butyrate induced autophagy via higher expression of Beclin-1 and LC3B-II. The AMP-activated protein kinase (AMPK) was downregulated along with the activation of Akt and mammalian target of rapamycin (mTOR) and decrease in acetyl-CoA carboxylase (ACC) in BR colon cancer cells compared to those in their respective PT cells. Activation of AMPK by AICAR treatment in BR colon cancer cells suppressed cell proliferation by inhibiting Akt and mTOR and activating ACC. Taken together, chronic exposure to butyrate increased butyrate resistance in human colon cancer by inducing protective autophagy through the downregulation of AMPK/ACC and activation of Akt/mTOR signaling. Activation of AMPK restored sensitivity to butyrate by the inhibition of Akt/mTOR, suggesting that AMPK could be a therapeutic target for BR colon cancers.
Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
Authors: Kotova Anastasia V. , Lobov Arseniy A. , Dombrovskaya Julia A. , Sannikova Valentina Y. , Ryumina Nadezhda A. , Klausen Polina , Shavarda Alexey L. , Malashicheva Anna B. , Enukashvily Natella I.
Published: November 2021

Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodontal ligament stem cells (PDLSC) in their pluripotency and neuroepithelial markers transcription, morphological and functional features, osteoblast/odontoblast differentiation and proteomic profile during osteogenic differentiation. The data were collected in paired observations: two cell cultures, DPSC and PDLSC, were obtained from each donor. Both populations had the mesenchymal stem cells surface marker set exposed on their membranes but differed in Nestin (a marker of neuroectodermal origin) expression, morphology, and proliferation rate. OCT4 mRNA was revealed in DPSC and PDLSC, while OCT4 protein was present in the nuclei of DPSC only. However, transcription of OCT4 mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. RUNX2 mRNA was detected by qPCR in both types of dental stem cells but RUNX2 protein was detected by LC-MS/MS shotgun proteomics only in PDLSC suggesting the posttranscriptional regulation. DSPP and DMP1, marker genes of odontoblastic type of osteogenic differentiation, were transcribed in DPSC but not in PDLSC samples. Our results prove that DPSC and PDLSC are different in their biology and therapeutic potential: DPSC are a good candidate for osteogenic or odontogenic bone-replacement cell-seeded medicines, while fast proliferating PDLSC are a prospective candidate for other cell products.
Hysteresis Envelope Model of Double Extended End-Plate Bolted Beam-to-Column Joint
Authors: Krolo Paulina , Grandić Davor
Published: November 2021

In this study, a hysteresis envelope mathematical model for the double extended end-plate bolted beam-to-column joint is proposed. The aim of a proposed joint model is to provide a more realistic behaviour of steel frames under seismic loading by using nonlinear static pushover analysis. The hysteresis envelope model defines the ratios between the monotonic properties of the joints and the properties of the joints during cyclic deformation. The proposed models are based on the hysteresis curves of the joints obtained by numerical simulations. The numerical model takes into account the geometric nonlinearity of the connecting elements, preloading of bolts, contacts between plates and bolts, and nonlinear properties of steel. Nonlinear static pushover analyses of steel frames are performed where the behaviour of the joints is described using the proposed hysteresis envelope models. The results are compared with the nonlinear static pushover analyses of steel frames with a trilinear monotonic joint model. Based on the results, the values of maximum peak ground acceleration for moment-resisting frames with the monotonic model of joints and hysteresis envelope model are estimated.
The Epidemiology of Osteomyelitis in Children
Authors: Walter Nike , Bärtl Susanne , Alt Volker , Rupp Markus
Published: November 2021

Pediatric osteomyelitis remains challenging to treat. Detailed epidemiological data are required to estimate future developments. Therefore, we aimed to analyze how the incidence has changed over the last decade depending on age, gender, osteomyelitis subtype, and anatomical localization. Cases were quantified for patients aged 20 years or younger, using yearly reported ICD-10 diagnosis codes from German medical institutions for the time period 2009 to 2019. Incidence rates of osteomyelitis increased by 11.7% from 8.2 cases per 100,000 children in 2009 to 9.2 cases per 100,000 children in 2019. The age-specific incidence rate revealed the highest occurrence of osteomyelitis in patients aged 10–15 years (15.3/100,000 children), which increased by 23% over the observation period, followed by the age group 5–10 years (9.7/100,000 children). In 2019, out of all diagnoses, 39.2% were classified as acute, 38.4% as chronic, and 22.4% were unspecified, whereby chronic cases increased by 38.7%. The lower extremity was mainly affected, with 58.9% of osteomyelitis diagnoses in 2019. In conclusion, pediatric osteomyelitis is a serious issue, even in a developed and industrialized country such as Germany. Considering the recent incidence increase, the permanent need for appropriate treatment should let pediatricians and orthopedic surgeons deal with diagnosis and treatment protocols.
The Implementation of Application Software to Improve Verbal Communication in Children with Autism Spectrum Disorder: A Review
Authors: Maseri Marlyn , Mamat Mazlina , Yew Hoe Tung , Chekima Ali
Published: November 2021

Autism-assistive apps offer therapists and caregivers new approaches for educating and assisting individuals with autism spectrum disorder (ASD), mainly in social interaction. Even though these apps are deemed effective, they are not. These autism-assistive apps are not highly customizable, which limits their usefulness. This article examined the application software that was applied to encourage verbal communication in the intervention for children with ASD. The aim was to determine the minimum requirements for a verbal communication intervention app that adequately satisfies children with ASD, caregivers, and therapists. Databases were searched, including Scopus, Springer, PubMed, Education Resources Information Centre, and Google Scholar, with the following free-text terms combining Boolean operators: autism, children, intervention, verbal communication, software, app, and technology. A total of fifteen studies were found relevant, and the following information was collected: participant characteristics, information on the devices and apps, target behaviors, intervention procedures, and intervention outcomes. The findings suggest that the autism-assistive apps effectively improve verbal communication of children with ASD. For that, the apps should be attractive and engaging to the children with ASD, able to identify the child’s capability and suggest appropriate lesson activities, as well as encompass specific learning outcomes with multilevel lesson strategy. The apps should also use systematic evidence-based intervention procedures in the activities, be able to evaluate the child’s learning progress, and allow caregivers or therapists to keep track of application usage and performance. The use of apps in intervention does provide many benefits. However, they should never replace qualified therapists. App-based interventions make home-based treatment more focused, systematic, and economical.