bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation of the Rational Range of Ecological Compensation to Address Land Degradation in the Poverty Belt around Beijing and Tianjin, China
Authors: Yan Haiming , Li Wei , Yang Huicai , Guo Xiaonan , Liu Xingran , Jia Wenru
Published: December 2021

Ecological compensation provides innovative ecological solutions for addressing land degradation and guaranteeing the sustainable provision of essential ecosystem services. This study estimated the ecosystem service value and the opportunity cost of land use in the Poverty Belt of China—around Beijing and Tianjin—from 1980 to 2015 on the small watershed scale, and thereafter estimated the rational range of ecological compensation in this ecologically fragile zone. Results showed that the total ecosystem service value in the study area gradually decreased from CNY 54.198 billion in 1980 to CNY 53.912 billion in 2015. Moreover, the annual total ecological compensation of the whole study area ranged between CNY 2.67 billion and 2.83 billion. More specifically, areas with higher ecological compensation standards are mainly concentrated in the northwestern and northern parts of the study area, with a lower economic development level, while areas with lower ecological compensation standards are mainly located in areas with a relatively high level of economic development, e.g., the southern and southeastern parts of the study area. These results can provide valuable decision-support information for the design and optimization of ecological compensation to address land degradation along with rapid urbanization in the Beijing–Tianjin–Hebei region.
Wear Behaviors of Carbon–Chromium Carbide–Chromium Multilayer Coatings Prepared by Reactive High-Power Impulse Magnetron Sputtering
Authors: Kuo Chin-Chiuan
Published: December 2021

Carbon–chromium carbide–chromium multilayer coatings were deposited by utilizing reactive high-power impulse magnetron sputtering with alternating various ratios of ethyne and argon mixtures under a constant total deposition pressure, target pulse frequency, pulse duty cycle, average chromium target power, and total deposition time. Two different alternating gas mixture periods were applied to obtain films with different numbers of layers and lamination thicknesses. The results show that the reduction in the modulation period effectively affects the elastic modulus and the subsequent ratio of hardness to elastic modulus (H/E) of the whole coating, which helps adapt the elastic strain in the coating. This improves the adhesion strength and wear resistance of coatings at room temperature. However, with the increase in wear test temperature, the difference between the wear behaviors of two types of coatings becomes inconspicuous. Both types of coatings lose the wear resistance due to the decomposition of hydrocarbon and the oxidation of the chromium content in the films.
Design of a Non-Contact Radial Torque Sensor with Variable Torque Range by Electromagnetic Coil Coupling with Piezoelectric Sensor
Authors: Wang Sheng-He
Published: December 2021

Recently, due to the development of automation technology, torque measuring and monitoring technologies have been brought to the focus. However, the commercially available sensors have the disadvantage of large volume, which results in the difficulty of installation on existing automated machines. Responding to the above-mentioned problem, a contactless torque sensor that uses an electromagnetic coil combined with a permanent magnet was proposed. By adjusting the input electric current in the coil, the strength of the magnetic field can be controlled to generate a non-contact magnetic force to resist external torque loading. For the measurement of such a magnetic force, a cantilever-beam mechanism comprising a piezoelectric-loading (PZT-L) sensor is employed to estimate the external static force by measuring the variation of the electric impedance. According to the measured results, the proposed PZT-L sensor demonstrates the accuracy of the proposed design, for which the maximum estimated error was around 6%. Finally, the proposed contactless torque sensor with 11 cm in diameter and 2 cm in thickness was employed to verify the effectiveness of theoretical analysis. From the sensor characteristic measurement, the detection range for external torque can be from 7.8 to 125.6 N-mm when the driven current input ranged from 2 to 10 A. Therefore, the experimental results presented that the moment of inertia via the resisted torque can be adjusted by the proposed non-contact torque-sensing system according to the measuring condition.
Development of Mechanical, Corrosion Resistance, and Antibacterial Properties of Steels
Authors: Conradi Marjetka
Published: December 2021

The total cost and environmental consequences of corrosion problems have become a major challenge to engineers [...]
Sustainable Development of Magnetic Chitosan Core–Shell Network for the Removal of Organic Dyes from Aqueous Solutions
Authors: Rathinam Karthik , Kou Xinwei , Hobby Ralph , Panglisch Stefan
Published: December 2021

The wide use of alizarin red S (ARS), a typical anthraquinone dye, has led to its continued accumulation in the aquatic environment, which causes mutagenic and carcinogenic effects on organisms. Therefore, this study focused on the removal of ARS dye by adsorption onto a magnetic chitosan core–shell network (MCN). The successful synthesis of the MCN was confirmed by ATR-FTIR, SEM, and EDX analysis. The influence of several parameters on the removal of ARS dye by the MCN revealed that the adsorption process reached equilibrium after 60 min, pH played a major role, and electrostatic interactions dominated for the ARS dye removal under acidic conditions. The adsorption data were described well by the Langmuir isotherm and a pseudo-second order kinetic model. In addition to the preferable adsorption of hydrophobic dissolved organic matter (DOM) fractions onto the MCN, the electrostatic repulsive forces between the previously adsorbed DOM onto MCN and ARS dye resulted in lower ARS dye removal. Furthermore, the MCN could easily be regenerated and reused for up to at least five cycles with more than 70% of its original efficiency. Most importantly, the spent MCN was pyrolytically converted into N-doped magnetic carbon and used as an adsorbent for various dyes, thus establishing a waste-free adsorption process.
Thermal Behavior of the Nimesulide-Salicylic Acid Eutectic Mixtures Prepared by Mechanosynthesis and Recrystallization
Authors: Georgescu Mona , Meltzer Viorica , Stănculescu Ioana , Pincu Elena
Published: December 2021

Nimesulide, salicylic acid and their binary mixtures were studied by differential scanning calorimetry (DSC) and Fourier Transform Infrared spectroscopy (FTIR). The study of such systems is a promising and viable approach for solving the problem of poor solubility of materials in general and drug systems in particular. All areas of human activity are inextricably linked to materials, and thus, the study presented in the paper and not reported in the literature is very important and provides useful data for those working in various fields. The eutectic mixtures were obtained by mechanosynthesis and by recrystallization from ethanol over the entire 0–1 range of molar fractions. For both situations at the molar fraction of nimesulide 0.5, the mixture has a eutectic that suggests an increase in solubility at this composition. The interactions that take place between the components were determined with the help of the excess thermodynamic functions (GE, SE, µE), which highlight the deviation from the ideality of the considered binary systems.
Effect of Diamond Polishing and Thermal Treatment on Carbon Paramagnetic Centers’ Nature and Structure
Authors: Litvak Ira , Cahana Avner , Anker Yaakov , Ruthstein Sharon , Cohen Haim
Published: December 2021

Diamonds contain carbon paramagnetic centers (stable carbon radicals) in small concentrations (at the level of ~1 × 1012 spins/mg) that can help in elucidating the structure of the nitrogen atoms’ contaminants in the diamond crystal. All diamonds that undergo polishing are exposed to high temperatures, owing to the friction force during the polishing process, which may affect the carbon-centered radicals’ concentration and structure. The temperature is increased appreciably; consequently, the black body radiation in the visible range turns orange. During polishing, diamonds emit an orange light (at a wavelength of about 600 nm) that is typical of a black body temperature of 900 °C or higher. Other processes in which color-enhanced diamonds are exposed to high temperatures are thermal treatments or the high-pressure, high-temperature (HPHT) process in which the brown color (resulting from plastic deformation) is bleached. The aim of the study was to examine how thermal treatment and polishing influence the paramagnetic centers in the diamond. For this purpose, four rough diamonds were studied: two underwent a polishing process, and the other two were thermally treated at 650 °C and 1000 °C. The diamonds were analyzed pre- and post-treatment by EPR (Electron Paramagnetic resonance), FTIR (Fourier transform infrared, fluorescence, and their visual appearance. The results indicate that the polishing process results in much more than just thermal heating the paramagnetic centers.
Effect of μPlasma Modification on the Wettability and the Ageing Behaviour of Glass Fibre Reinforced Polyamide 6 (GFPA6)
Authors: Che Chang , Dashtbozorg Behnam , Li Xiaoying , Dong Hanshan , Jenkins Mike
Published: December 2021

Glass fibre reinforced polyamide 6 (GFPA6) thermoplastic composites (TPCs) are promising materials with excellent properties, but due to their low surface free energy they are usually difficult to wet, and therefore, possesses poor adhesion properties. μPlasma modification offers potential solutions to this problem through functionalisation of the GFPA6 surface. In this study, the effect of μPlasma on the wetting behaviour of GFPA6 surfaces was investigated. Following single μPlasma treatment scans of GFPA6 samples, a substantial enhancement in wettability was observed. However, the effect of the μPlasma modification was subject to an ageing (hydrophobic recovery) phenomenon, although the enhancement was still partially maintained after 4 weeks. The ageing process was slower when the GFPA6 material was pre-dried and stored in low humidity conditions, thereby demonstrating the importance of the storage environment to the rate of ageing. Orientation of the fibres to the observed contact angle was found to be crucial for obtaining reproducible measurements with lower deviation. The influence of testing liquid, droplet volume and surface texture on the repeatability of the measured contact angle were also investigated.
A Novel Auction Blockchain System with Price Recommendation and Trusted Execution Environment
Authors: Shih Dong-Her , Wu Ting-Wei , Shih Ming-Hung , Tsai Wei-Cheng , Yen David C.
Published: December 2021

Online auctions are now widely used, with all the convenience and efficiency brought by internet technology. Despite the advantages over traditional auction methods, some challenges still remain in online auctions. According to the World Business Environment Survey (WBES) conducted by the World Bank, about 60% of companies have admitted to bribery and manipulation of the auction results. In addition, buyers are prone to the winner’s curse in an online auction environment. Since the increase in information availability can reduce uncertainty, easy access to relevant auction information is essential for buyers to avoid the winner’s curse. In this study, we propose an Online Auction Price Suggestion System (OAPSS) to protect the data from being interfered with by third-party programs based on Intel’s Software Guard Extensions (SGX) technology and the characteristics of the blockchain. Our proposed system provides a smart contract by using α-Sutte indicator in the final transaction price prediction as a bidding price recommendation, which helps buyers to reduce the information uncertainty on the value of the product. The amount spent on the smart contract in this study, excluding deployed contracts, plus the rest of the fees is less than US$1. Experimental results of the simulation show that there is a significant difference (p < 0.05) between the recommended price group and the actual price group in the highest bid. Therefore, we may conclude that our proposed bidder’s price recommendation function in the smart contract may mitigate the loss of buyers caused by the winner’s curse.
Applications of Discrete Wavelet Transform for Feature Extraction to Increase the Accuracy of Monitoring Systems of Liquid Petroleum Products
Authors: Balubaid Mohammed , Sattari Mohammad Amir , Taylan Osman , Bakhsh Ahmed A. , Nazemi Ehsan
Published: December 2021

This paper presents a methodology to monitor the liquid petroleum products which pass through transmission pipes. A simulation setup consisting of an X-ray tube, a detector, and a pipe was established using a Monte Carlo n-particle X-version transport code to investigate a two-by-two mixture of four different petroleum products, namely, ethylene glycol, crude oil, gasoline, and gasoil, in deferent volumetric ratios. After collecting the signals of each simulation, discrete wavelet transform (DWT) was applied as the feature extraction system. Then, the statistical feature, named the standard deviation, was calculated from the approximation of the fifth level, and the details of the second to fifth level provide appropriate inputs for neural network training. Three multilayer perceptron neural networks were utilized to predict the volume ratio of three types of petroleum products, and the volume ratio of the fourth product could easily be obtained from the results of the three presented networks. Finally, a root mean square error of less than 1.77 was obtained in predicting the volume ratio, which was much more accurate than in previous research. This high accuracy was due to the use of DWT for feature extraction.
Numerical Simulation of Failure Analysis of Storage Tank with Partition Plate and Structure Optimization
Authors: Su Wenxian , Feng Xiao
Published: December 2021

Storage tanks with partition plates are widely used in the petrochemical industry. However, relevant standards do not propose corresponding design criteria and methods for this type of structure, and theoretical design formulas cannot be applied to ensure the reliability of its structure. Therefore, it is necessary to analyze and design the storage tank with a partition plate by using finite elements. This paper studies the problem of buckling depression and cracks in the welded parts of the S-shaped tank with a partition plate during its operation. We used the finite element software ANSYS to analyze the overall strength and stability of the structure and obtain the larger stress area. Based on this, a safe and economical optimization plan is proposed: under the condition of strictly controlling the liquid level difference on both sides of the partition, the tank structure is optimized by adding stiffeners and tie rods. The study revealed that the measure effectively improves the overall rigidity of the tank body and reduces the maximum stress of the structure and enhances the safety performance of storage tank. Additionally, it provides a reference for the structural strength design of storage tanks with partition plates.
Improvement of Trajectory Tracking by Robot Manipulator Based on a New Co-Operative Optimization Algorithm
Authors: Elsisi Mahmoud , Zaini Hatim G. , Mahmoud Karar , Bergies Shimaa , Ghoneim Sherif S. M.
Published: December 2021

The tracking of a predefined trajectory with less error, system-settling time, system, and overshoot is the main challenge with the robot-manipulator controller. In this regard, this paper introduces a new design for the robot-manipulator controller based on a recently developed algorithm named the butterfly optimization algorithm (BOA). The proposed BOA utilizes the neighboring butterflies’ co-operation by sharing their knowledge in order to tackle the issue of trapping at the local optima and enhance the global search. Furthermore, the BOA requires few adjustable parameters via other optimization algorithms for the optimal design of the robot-manipulator controller. The BOA is combined with a developed figure of demerit fitness function in order to improve the trajectory tracking, which is specified by the simultaneous minimization of the response steady-state error, settling time, and overshoot by the robot manipulator. Various test scenarios are created to confirm the performance of the BOA-based robot manipulator to track different trajectories, including linear and nonlinear manners. Besides, the proposed algorithm can provide a maximum overshoot and settling time of less than 1.8101% and 0.1138 s, respectively, for the robot’s response compared to other optimization algorithms in the literature. The results emphasize the capability of the BOA-based robot manipulator to provide the best performance compared to the other techniques.
Combination Therapy with Rituximab, Tofacitinib and Pirfenidone in a Patient with Rapid Progressive Interstitial Lung Disease (RP-ILD) Due to MDA5 Antibody-Associated Dermatomyositis: A Case Report
Authors: Yen Tsai-Hung , Tseng Chih-Wei , Wang Kao-Lun , Fu Pin-Kuei
Published: December 2021

Anti-melanoma differentiation-associated protein 5 (MDA5)-positive rapidly progressive interstitial lung disease (RP-ILD) is associated with poor prognosis, and the most effective therapeutic intervention has not been established. Herein we report a case of a 45-year-old female patient who presented with myalgia, Gottron’s papules with ulceration, and dyspnea on exertion which became aggravated within weeks. Laboratory examination and electromyography confirmed myopathy changes, and a survey of myositis-specific antibodies was strongly positive for anti-MDA5 antibody. High-resolution chest tomography suggested organizing pneumonia with rapidly progressive changes within the first month after diagnosis of the disease. Anti-MDA5-associated dermatomyositis with RP-ILD was diagnosed. Following combination therapy with rituximab, tofacitinib and pirfenidone, clinical symptoms, including cutaneous manifestation, respiratory conditions and radiographic changes, showed significant and sustainable improvement. To our knowledge, this is the first reported case of anti-MDA5-associated dermatomyositis with RP-ILD successfully treated with the combination of rituximab, tofacitinib, and pirfenidone.
Carbide Precipitation in a Low Alloyed Steel during Aging Studied by Atom Probe Tomography and Thermodynamic Modeling
Authors: Thuvander Mattias , Magnusson Hans , Borggren Ulrika
Published: December 2021

Carbide precipitation in martensitic low alloyed steels contributes to the mechanical properties through precipitation hardening. A high number density of carbides is desired to maximize the hardening effect, which is achieved through the precipitation of carbides on the dislocations in the martensitic structure. In this study, the nucleation, growth, and coarsening of vanadium and molybdenum carbides during aging at 600 °C for periods up to four weeks were investigated. The work covers characterization with atom probe tomography, which showed that the nucleation of V and Mo rich MC/M2C carbides takes place on dislocations. The growth of these carbides proceeds by the diffusion of elements to the dislocations, which has been modeled using Dictra software, confirming the rate of the reaction as well as the depletion of carbide formers in the matrix. For longer aging times, particle coarsening will decrease the number density of particles with a transition from dislocation-based carbides to separate rounded carbides.
Numerical Study of Epitaxial Growth after Partial Remelting during Selective Electron Beam Melting in the Context of Ni–Al
Authors: Schaar Helge , Steinbach Ingo , Tegeler Marvin
Published: December 2021

In the selective electron beam melting approach an electron beam is used to partially melt the material powder. Based on the local high energy input, the solidification conditions and likewise the microstructures strongly deviate from conventional investment casting processes. The repeated energy input into the material during processing leads to the partial remelting of the already existing microstructure. To closer investigative this effect of partial remelting, in the present work the phase-field model is applied. In the first part the solidification of the referenced Ni–Al system is simulated in respect to selective electron beam melting. The model is calibrated such to reproduce the solidification kinetics of the superalloy CMSX-4. By comparison to experimental observations reported in the literature, the model is validated and is subsequently applied to study the effect of partial remelting. In the numerical approach the microstructures obtained from the solidification simulations are taken as starting condition. By systematically varying the temperature of the liquid built layer, the effect of remelting on the existing microstructure can be investigated. Based on these results, the experimental processing can be optimized further to produce parts with significantly more homogenous element distributions.
The Fabrication and Mechanical Properties of Laminated ZrB2-Mo5SiB2 Ceramics with an Mo-Mo5SiB2 Interlayer
Authors: Wang Yanfang , Li Mingliang , Wang Hailong , Shao Gang , Zhu Jinpeng , Liu Wen , Wang Hailiang , Fan Bingbing , Xu Hongliang , Lu Hongxia , Zhang Rui
Published: December 2021

The excellent physical and chemical properties of ultra-high temperature ceramics make them suitable for many high-temperature structural components, while their poor toughness and high sintering temperature become key limitations to their application. Laminated toughening has long been considered an effective toughening method to improve the mechanical properties of ceramics. In this study, laminated ZrB2-Mo5SiB2 ceramics with an Mo-Mo5SiB2 interlayer were fabricated by tape casting and hot press sintering at 1900 °C for 2 h. Different layer thickness ratios between the matrix layer and the interlayer were designed to illustrate the toughening mechanism. Both the fracture toughness and flexural strength of the laminated ceramics showed a trend of first increasing and then decreasing with the increase of the layer thickness ratio. High fracture toughness (9.89 ± 0.26 MPa·m1/2) and flexural strength (431.6 ± 15.1 MPa) were obtained when the layer thickness ratio was 13. The improvement in fracture toughness of the laminated ceramics could be attributed to the generation of the residual stress, the deflection and the bifurcation of the cracks. Residual stress that developed in the laminated ceramics was also evaluated.
SiGe/Si Multi-Quantum-Well Micro-Bolometer Array Design and Fabrication with Heterogeneous Integration
Authors: Fang Zhong , He Yong , Chen Zhequan , Shi Yunlei , Jiao Junjie , Pan Xuchao
Published: December 2021

The micro-bolometer is important in the field of infrared imaging, although improvements in its performance have been limited by traditional materials. SiGe/Si multi-quantum-well materials (SiGe/Si MQWs) are novelty thermal-sensitive materials with a significantly high TCR and a comparably low 1/f noise. The application of such high-performance monocrystalline films in a micro-bolometer has been limited by film integration technology. This paper reports a SiGe/Si MQWs micro-bolometer fabrication with heterogeneous integration. The integration with the SiGe/Si MQWs handle wafer and dummy read-out circuit wafer was achieved based on adhesive wafer bonding. The SiGe/Si MQWs infrared-absorption structure and thermal bridge were calculated and designed. The SiGe/Si MQWs wafer and a 320 × 240 micro-bolometer array of 40 µm pitch L-type pixels were fabricated. The test results for the average absorption efficiency were more than 90% at the wavelength of 8–14 µm. The test pixel was measured to have a thermal capacity of 1.043 × 10−9 J/K, a thermal conductivity of 1.645 × 10−7 W/K, and a thermal time constant of 7.25 ms. Furthermore, the total TCR value of the text pixel was measured as 2.91%/K with a bias voltage of 0.3 V. The SiGe/Si MQWs micro-bolometer can be widely applied in commercial fields, especially in early medical diagnosis and biological detection.
Preparation, Structure, and Electrical Properties of Cobalt-Modified Bi(Sc3/4In1/4)O3–PbTiO3–Pb(Mg1/3Nb2/3)O3 High-Temperature Piezoelectric Ceramics
Authors: Chen Zhijiang , Lin Na , Yang Zhao , Zhang Juan , Shi Kefei , Sun Xinhao , Gao Bo , Zhao Tianlong
Published: December 2021

Cobalt-modified 0.40Bi(Sc3/4In1/4)O3–0.58PbTiO3–0.02Pb(Mg1/3Nb2/3)O3 ceramics (abbreviated as BSI–PT–PMN–xCo) were produced by conventional two-step solid-state processing. The phase structure, micro structure morphology, and electrical properties of BSI–PT–PMN–xCo were systematically studied. The introduction of Co ions exerted a significant influence on the structure and electrical properties. The experiment results demonstrated that Co ions entered the B-sites of the lattice, resulting in slight lattice distortion and a smaller lattice constant. The average grain size increased from ~1.94 μm to ~2.68 μm with the increasing Co content. The optimized comprehensive electrical properties were obtained with proper Co-modified content 0.2 wt.%. The Curie temperature (Tc) was 412 °C, the piezoelectric constant (d33) was 370 pC/N, the remnant polarization (Pr) was 29.2 μC/cm2, the relatively dielectric constant (εr) was 1450, the planar electromechanical coupling coefficient (kp) was 46.5, and the dielectric loss (tanδ) was 0.051. Together with the enhanced DC resistivity of 109 Ω cm under 300 °C and good thermal stability, BSI–PT–PMN–0.2Co ceramic is a promising candidate material for high-temperature piezoelectric applications.
Lactobacillus iners Cell-Free Supernatant Enhances Biofilm Formation and Hyphal/Pseudohyphal Growth by Candida albicans Vaginal Isolates
Authors: Sabbatini Samuele , Visconti Sofia , Gentili Marco , Lusenti Eleonora , Nunzi Emilia , Ronchetti Simona , Perito Stefano , Gaziano Roberta , Monari Claudia
Published: December 2021

Candida albicans is a commensal fungus of the vaginal mucosa and the principal etiological agent of vaginal candidiasis. Vaginal dysbiosis has been reported during vulvovaginal candidiasis (VVC), with a progressive decrease in Lactobacillus crispatus population and an increase in L. iners population. To date, the role of L. iners in VVC pathogenesis remains scarcely explored. Herein we investigated the in vitro effect of L. iners cell-free supernatant (CFS) on the ability of C. albicans to form biofilms. Biomass and metabolic activity were measured by crystal violet and XTT assays. Further, light microscopy was performed to determine the effect of L. iners CFS on biofilm cellular morphology. We found that L. iners CFS induced a significant increase in biofilm formation by C. albicans clinical isolates which were categorized as moderate or weak biofilm producers. This effect was associated with an enhancement of hyphal/pseudohyphal growth, and the expression levels of HWP1 and ECE1, which are typical hyphae-associated genes, were upregulated. Overall, these results suggest that L. iners contributes to the pathogenesis of VVC and highlight the complexity of the interaction between C. albicans and vaginal lactobacilli. Understanding these interactions could prove essential for the development of new strategies for treating VVC.
Microbial Community Successional Changes in a Full-Scale Mesophilic Anaerobic Digester from the Start-Up to the Steady-State Conditions
Authors: Tonanzi Barbara , Crognale Simona , Gianico Andrea , Della Sala Stefano , Miana Paola , Zaccone Maria Chiara , Rossetti Simona
Published: December 2021

Anaerobic digestion is a widely used technology for sewage sludge stabilization and biogas production. Although the structure and composition of the microbial communities responsible for the process in full-scale anaerobic digesters have been investigated, little is known about the microbial successional dynamics during the start-up phase and the response to variations occurring in such systems under real operating conditions. In this study, bacterial and archaeal population dynamics of a full-scale mesophilic digester treating activated sludge were investigated for the first time from the start-up, performed without adding external inoculum, to steady-state operation. High-throughput 16S rRNA gene sequencing was used to describe the microbiome evolution. The large majority of the reads were affiliated to fermentative bacteria. Bacteroidetes increased over time, reaching 22% of the total sequences. Furthermore, Methanosaeta represented the most abundant methanogenic component. The specific quantitative data generated by real-time PCR indicated an enrichment of bacteria and methanogens once the steady state was reached. The analysis allowed evaluation of the microbial components more susceptible to the shift from aerobic to anaerobic conditions and estimation of the microbial components growing or declining in the system. Additionally, activated sludge was investigated to evaluate the microbial core selected by the WWTP operative conditions.
Isolation and Identification of Wild Yeast from Malaysian Grapevine and Evaluation of Their Potential Antimicrobial Activity against Grapevine Fungal Pathogens
Authors: Sabaghian Simin , Braschi Giacomo , Vannini Lucia , Patrignani Francesca , Samsulrizal Nurul Hidayah , Lanciotti Rosalba
Published: December 2021

Pathogenic fungi belonging to the genera Botrytis, Phaeomoniella, Fusarium, Alternaria and Aspergillus are responsible for vines diseases that affect the growth, grapevine yield and organoleptic quality. Among innovative strategies for in-field plant disease control, one of the most promising is represented by biocontrol agents, including wild epiphytic yeast strains of grapevine berries. Twenty wild yeast, isolated and molecularly identified from three different Malaysian regions (Perlis, Perak and Pahang), were evaluated in a preliminary screening test on agar to select isolates with inhibition against Botrytis cinerea. On the basis of the results, nine yeasts belonging to genera Hanseniaspora, Starmerella, Metschnikowia, Candida were selected and then tested against five grape berry pathogens: Aspergillus carbonarius, Aspergillus ochraceus, Fusarium oxysporum, Alternaria alternata and Phaeomoniella chlamydospora.Starmerella bacillaris FE08.05 and Metschnikowia pulcherrima GP8 and Hanseniaspora uvarum GM19 showed the highest effect on inhibiting mycelial growth, which ranged between 15.1 and 4.3 mm for the inhibition ring. The quantitative analysis of the volatile organic compound profiles highlighted the presence of isoamyl and phenylethyl alcohols and an overall higher presence of low-chain fatty acids and volatile ethyl esters. The results of this study suggest that antagonist yeasts, potentially effective for the biological control of pathogenic moulds, can be found among the epiphytic microbiota associated with grape berries.
Evaluating the Potential for Smoke from Stubble Burning to Taint Grapes and Wine
Authors: Wilkinson Kerry , Ristic Renata , McNamara Imogen , Loveys Beth , Jiang WenWen , Krstic Mark
Published: December 2021

It has been well established that bushfire/wildfire smoke can taint grapes (and therefore wine), depending on the timing and duration of exposure, but the risk of smoke contamination from stubble burning (a practice employed by some grain growers to prepare farmland for sowing) has not yet been established. This study exposed excised bunches of grapes to smoke from combustion of barley straw and pea stubble windrows to investigate the potential for stubble burning to elicit smoke taint. Increased levels of volatile phenols (i.e., chemical markers of smoke taint) were detected in grapes exposed to barley straw smoke (relative to control grapes), with smoke density and the duration of smoke exposure influencing grape volatile phenols. However, the sensory panel did not perceive wine made from grapes exposed to low-density smoke to be tainted, despite the presence of low levels of syringol providing compositional evidence of smoke exposure. During the pea stubble burn, grapes positioned amongst the burning windrows or on the edge of the pea paddock were exposed to smoke for ~15–20 and 30–45 min, respectively, but this only resulted in 1 µg/kg differences in the cresol and/or syringol concentrations of smoke-affected grapes (and 1 µg/L differences for wine), relative to controls. A small, but significant increase in the intensity of smoke aroma and burnt rubber flavor of wine made from the grapes positioned amongst the burning pea stubble windrows provided the only sensory evidence of any smoke taint. As such, had vineyards been located immediately downwind from the pea stubble burn, it is unlikely that there would have been any smoke contamination of unharvested grapes.
Solubility of Hybrid Halide Perovskites in DMF and DMSO
Authors: Petrov Andrey A. , Ordinartsev Artem A. , Fateev Sergey A. , Goodilin Eugene A. , Tarasov Alexey B.
Published: December 2021

Solution methods remain the most popular means for the fabrication of hybrid halide perovskites. However, the solubility of hybrid perovskites has not yet been quantitively investigated. In this study, we present accurate solubility data for MAPbI3, FAPbI3, MAPbBr3 and FAPbBr3 in the two most widely used solvents, DMF and DMSO, and demonstrate huge differences in the solubility behavior depending on the solution compositions. By analyzing the donor numbers of the solvents and halide anions, we rationalize the differences in the solubility behavior of hybrid perovskites with various compositions, in order to take a step forward in the search for better processing conditions of hybrid perovskites for solar cells and optoelectronics.
Enhancement of the SPR Effect in an Optical Fiber Device Utilizing a Thin Ag Layer and a 3092A Liquid Crystal Mixture
Authors: Korec Joanna , Stasiewicz Karol A. , Garbat Katarzyna , Jaroszewicz Leszek R.
Published: December 2021

This paper is a continuation of previous work and shows the enhancement of the surface plasmon resonance effect in a tapered optical fiber device. The study investigated liquid crystal cells containing a tapered optical fiber covered with a silver nanolayer, surrounded by a low refractive index liquid crystal in terms of the properties of light propagation in the taper structure. Silver films with a thickness of d = 10 nm were deposited on the tapered waist area. Measurements were performed at room temperature; liquid crystal steering voltage U from 0 to 200 V, with and without any amplitude modulation with a frequency of f = 5 Hz, and the wavelength λ ranged from 550 to 1200 nm. A significant influence of the initial arrangement of liquid crystals molecules on light propagation was observed. Three types of liquid crystal cells—orthogonal, parallel, and twist—were considered. During the measurements, resonant peaks were obtained—the position of which can also be controlled by the type of liquid crystal cells and the steering voltage. Based on the obtained results, the best parameters, such as highest peak’s width reduction, and the highest SNR value were received for twisted cells. In addition, the present work was compared with the previous work and showed the possibility of improving properties of the manufactured probes, and consequently, the surface plasmon resonance effect. In the presented paper, the novelty is mainly focused on the used materials as well as suitable changes in applied technological parameters. In contrast to gold, silver is characterized by different optic and dielectric properties, e.g., refractive index, extension coefficient, and permittivity, which results in changes in the light propagation and the SPR wavelengths.
Secondary Metabolites with Cytotoxic Activities from Streptomyces sp. BM-8 Isolated from the Feces of Equusquagga
Authors: Lu Shengsheng , Hu Jianan , Xie Xi , Zhou Runhong , Li Fangfang , Huang Ruifeng , He Jian
Published: December 2021

A new aliphatic acid, compound 1, together with six known metabolites, including nonactic acid (2), homononactic acid (3), ethyl homononactate (4), homononactylhomononactate (5), valinomycin (6), and cyclo-(Pro-Leu) (7), was isolated from the culture broth of Streptomyces sp. BM-8, an actinobacterial strain isolated from the feces of Equus quagga. The structures of these compounds were established by analyses of spectroscopic data, including 1D and 2D nuclear magnetic resonance spectra (NMR), as well as by HR-ESI-MS spectrometry and chemical derivative analyses. Additionally, a serial analogue of nonactic acid and homononacticacid (8–21) was synthesized. The cytotoxicity of 1–21 wastested against a panel of cancer cell lines, such as HT-29, MCF-7, A375 and K562, with MTT assay. In addition, the cytotoxicity tests revealed that 1 was less cytotoxic toward a panel of cancerous cells, as compared with valinomycin (6).
Microbial Diversity and Characteristics of Kombucha as Revealed by Metagenomic and Physicochemical Analysis
Authors: Kaashyap Mayank , Cohen Marc , Mantri Nitin
Published: December 2021

Kombucha is a fermented tea made from a Symbiotic Culture of Bacteria and Yeast (SCOBY) with a long history of use as a health tonic. It is likely that most health benefits come from the tea and fermentation metabolites from specific microbial communities. Despite its growing importance as a functional health drink, the microbial ecosystem present in kombucha has not been fully documented. To characterize the microbial composition and biochemical properties of ‘The Good Brew’ original base kombucha, we used metagenomics amplicon (16S rRNA and ITS) sequencing to identify the microbial communities at the taxonomic level. We identified 34 genera with 200 microbial species yet described in kombucha. The dominance of organic acid producing microorganisms Acetobacter, Komagataeibacter and Starmerella are healthy for the human gut and their glucose metabolising activities have a putative role in preventing conditions such as diabetes and obesity. Kombucha contains high protein (3.31 µg/mL), high phenolic content (290.4 mg/100 mL) and low sugars (glucose: 1.87 g/L; sucrose 1.11 g/L; fructose: 0.05 g/L) as compared to green tea. The broad microbial diversity with proven health benefits for the human gut suggests kombucha is a powerful probiotic. These findings are important to improve the commercial value of kombucha and uncover the immense prospects for health benefits.
A Pilot, Phase II, Observational, Case-Control, 1-Month Study on Asthenopia in Video Terminal Operators without Dry Eye: Contrast Sensitivity and Quality of Life before and after the Oral Consumption of a Fixed Combination of Zinc, L-Carnitine, Extract of Elderberry, Currant and Extract of Eleutherococcus.
Authors: Rossi Gemma Caterina Maria , Scudeller Luigia , Bettio Federica , Milano Giovanni
Published: December 2021

The aims of the study were to investigate the ability and effectiveness of an oral intake of a fixed combination of zinc, L-carnitine, elderberry extract, black currant and Eleutherococcus extract in controlling the symptoms of eyestrain in videoterminal (VDT) users and to record its effects on contrast sensitivity. A single-center, phase II, observational, case-control, 1-month study in VDT workers without dry eye disease was carried out. Demographics and number of actual hours at VDT/day were taken into account. All subjects underwent a complete ophthalmic examination, including assessment of contrast sensitivity, and completed the computer vision symptom scale questionnaire at baseline and one month later. A total of 30 Caucasian subjects adhered to the required inclusion criteria and completed the study; 15 subjects were treated (T) and 15 were controls (C). All clinical data at baseline were similar in both groups (p > 0.05): after one month, all subjects had stable visual acuity, refractive defect and intraocular pressure (IOP); screen exposure time was unchanged. Regarding symptoms, at randomization, the groups had a similar score: 33.1 ± 3.3 in T and 32.8 ± 5.6 in C. One month later, the computer vision symptom scale (CVSS) questionnaire score decreased by −14.1 ± 3, 1 (p = 0.000) and −2.3 ± 1.8 (p = 0.568), respectively. Regarding contrast sensitivity, in group C the values of spatial frequencies remained unchanged, while they improved in almost all the cycles per degree stimuli in the treated group. Oral intake of a fixed combination of zinc, L-carnitine, elderberry extract, black currant and eleutherococcus extract can significantly improve contrast sensitivity and symptoms in VDT workers with no signs of dry eye disease.
Effect of Nutrition Education in NAFLD Patients Undergoing Simultaneous Hyperlipidemia Pharmacotherapy: A Randomized Controlled Trial
Authors: Lee Won Myung , Bae Jea Hurn , Chang Young , Lee Sae Hwan , Moon Ji Eun , Jeong Soung Won , Jang Jae Young , Kim Sang Gyune , Kim Hong Soo , Yoo Jeong-Ju , Kim Young Seok
Published: December 2021

Background: Patients with non-alcoholic fatty liver disease (NAFLD) have a high prevalence of combined hyperlipidemia. The importance of nutritional education is well-known in NAFLD, but the impact of medical nutrition therapy (MNT) is unclear in patients with NAFLD with hyperlipidemia. The purpose of this study is to investigate the effect of MNT on the improvement of steatohepatitis in patients with NAFLD taking antihyperlipidemic medications. Methods: Nondiabetic patients with dyslipidemia were prospectively randomized (1:1) either to the MNT group or the control group with standard advice for 48 weeks with simultaneous statin/ezetimibe combination pharmacotherapy at three tertiary centers in Korea. Results: Sixty-six patients were enrolled. Among them, 18 patients dropped out and, overall, 48 patients (MNT group 27, control group 21) were prospectively analyzed in the study. The serum ALT level at 48 weeks between the two groups was not significantly different (66.6 ± 37.7 IU/L vs. 57.4 ± 36.7 IU/L, p = 0.40). Serum liver enzymes, controlled attenuation parameter and fibrosis-4 index were significantly improved within the MNT group after 48 weeks compared to baseline. There was no significant difference between the two groups other than the NAFLD fibrosis score (p = 0.017). Conclusions: Although there were no significant differences between the two groups in terms of steatosis, metabolic and fibrosis surrogate indicators after 48 weeks, MNT groups showed significant improvement within patient analysis over time. Future studies with a larger number of subjects and a longer study period regarding the effect of MNT are warranted.
DANCR Mediates the Rescuing Effects of Sesamin on Postmenopausal Osteoporosis Treatment via Orchestrating Osteogenesis and Osteoclastogenesis
Authors: Yang Zhengmeng , Feng Lu , Wang Haixing , Li Yucong , Lo Jessica Hiu Tung , Zhang Xiaoting , Lu Xuan , Wang Yaofeng , Lin Sien , Tortorella Micky D. , Li Gang
Published: December 2021

As one of the leading causes of bone fracture in postmenopausal women and in older men, osteoporosis worldwide is attracting more attention in recent decades. Osteoporosis is a common disease mainly resulting from an imbalance of bone formation and bone resorption. Pharmaceutically active compounds that both activate osteogenesis, while repressing osteoclastogenesis hold the potential of being therapeutic medications for osteoporosis treatment. In the present study, sesamin, a bioactive ingredient derived from the seed of Sesamum Indicum, was screened out from a bioactive compound library and shown to exhibit dual-regulating functions on these two processes. Sesamin was demonstrated to promote osteogenesis by upregulating Wnt/β-catenin, while repressing osteoclastogenesis via downregulating NF-κB signaling . Furthermore, DANCR was found to be the key regulator in sesamin-mediated bone formation and resorption . In an ovariectomy (OVX)-induced osteoporotic mouse model, sesamin could rescue OVX-induced bone loss and impairment. The increased serum level of DANCR caused by OVX was also downregulated upon sesamin treatment. In conclusion, our results demonstrate that sesamin plays a dual-functional role in both osteogenesis activation and osteoclastogenesis de-activation in a DANCR-dependent manner, suggesting that it may be a possible medication candidate for osteoporotic patients with elevated DNACR expression levels.
Aberrance of Zinc Metalloenzymes-Induced Human Diseases and Its Potential Mechanisms
Authors: Cheng Yunqi , Chen Hongping
Published: December 2021

Zinc, an essential micronutrient in the human body, is a component in over 300 enzymes and participates in regulating enzymatic activity. Zinc metalloenzymes play a crucial role in physiological processes including antioxidant, anti-inflammatory, and immune responses, as well as apoptosis. Aberrant enzyme activity can lead to various human diseases. In this review, we summarize zinc homeostasis, the roles of zinc in zinc metalloenzymes, the physiological processes of zinc metalloenzymes, and aberrant zinc metalloenzymes in human diseases. In addition, potential mechanisms of action are also discussed. This comprehensive understanding of the mechanisms of action of the regulatory functions of zinc in enzyme activity could inform novel zinc-micronutrient-supply strategies for the treatment of diseases.
Monitoring Sexually Transmitted Infections in Cervicovaginal Exfoliative Samples in Mexican Women
Authors: Hernández-Rosas Fabiola , Rey-Barrera Manuel , Conejo-Saucedo Ulises , Orozco-Hernández Erika , Maza-Sánchez Liliana , Navarro-Vidal Enrique , López-Vera Yasmín , Ascencio-Gordillo María del Carmen , de León-Bautista Mercedes Piedad
Published: December 2021

Background. Globally, Sexually Transmitted Infections (STIs) are a major cause of morbidity in sexually active individuals, having complications in reproduction health and quality of life. In concordance with the Sustainable Development Goals (SDG), the study aimed to investigate the prevalence of Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, Chlamydia trachomatis, HSV, and Mycoplasma spp. from cervicovaginal samples and to correlate them with the gynecological history of the patients. Methods. Our analytical, prospective, and cross-sectional study included 377 women who participated in a reproductive health campaign during 2015–2016. Anthropometric and gynecological variables were obtained. Cervicovaginal specimens were collected and analyzed with a multiplex in-house PCR to detect Candida spp., Ureaplasma spp., Trichomonas vaginalis, Neisseria gonorrhoeae, HSV, Mycoplasma spp., and Chlamydia trachomatis. Results. The positive cases were 175/377 (46.4%) to at least one of the microorganisms. The most frequent pathogen detected in this population was Ureaplasma spp. (n = 111, 29.4%), followed by Mycoplasma spp. (n = 56, 14.9%) and Candida spp. (n = 47, 12.5%); 33.7% of the positive cases were single infections, whereas 12.7% had coinfection. The multiplex PCR assay was designed targeting nucleotide sequences. Conclusions. Our data demonstrated that monitoring STIs among asymptomatic patients will encourage target programs to be more precisely and effectively implemented, as well as make these programs more affordable, to benefit society by decreasing the prevalence of STIs.