bluuebunny/crossref_metadata_2025 huggingface dataset:
To advise and scrutinize the government? Two types of political knowledge, political trust and unconventional participation
Authors: Michalski Piotr , Marchlewska Marta , Furman Aleksandra , Szczepańska Dagmara , Panayiotou Orestis , Molenda Zuzanna , Górska Paulina
Published: December 2021

Well-functioning democracies depend on citizens’ ability to make accurate political judgments and express them in the public sphere. Thus, in this research, we aim to better understand the role of political knowledge and political trust in shaping young Poles’ willingness to engage in unconventional participation such as signing a petition, boycotting specific products, taking part in a peaceful demonstration, or engaging in social media activities. We distinguish between two types of political knowledge: knowledge about the rules of the game and current political knowledge, which provide a more insightful look into the complex nature of relationships between political knowledge, political trust, and unconventional participation. In two studies (Study 1, N = 570 and Study 2; N = 1048) we found that unconventional participation was positively predicted by political trust, and political knowledge about the rules of the game. We also found a significant interaction effect between political trust and current political knowledge, suggesting that those high in current political knowledge may be more willing to participate only when being distrustful towards the current political system. Implications and limitations of these findings are discussed.
Prostate-specific membrane antigen and fibroblast activation protein distribution in prostate cancer: preliminary data on immunohistochemistry and PET imaging
Authors: Kessel Katharina , Seifert Robert , Weckesser Matthias , Boegemann Martin , Huss Sebastian , Kratochwil Clemens , Haberkorn Uwe , Giesel Frederik , Rahbar Kambiz
Published: December 2021

Introduction

Fibroblast activation protein (FAP) has been recently presented as new imaging target for malignant diseases and offers high contrast to surrounding normal tissue. FAP tracer uptake has been reported in various tumor entities. The aim of this study was to compare FAP and Prostate-specific membrane antigen (PSMA) expression in primary prostate cancer employing histological analyses and PET imaging in two small patient collectives.

Methods

Two independent small patient collectives were included in this study. For cohort A, data of 5 prostate cancer patients and 3 patients with benign prostate hyperplasia were included. Patients with prostate cancer were initially referred for PSMA PET staging. Radical prostatectomy was performed in all patients and prostate specimen of patients and biopsies of healthy controls were available for further evaluation. Histological workup included HE and immunohistochemistry using PSMA Ab, FAP Ab. Cohort B consists of 6 Patients with diagnosed mCRPC and available PSMA as well as FAP PET.

Results

Patients with proven prostate cancer infiltration exhibited strong positivity for PSMA in both primary tumors and lymph node metastases while stainings for FAP were found positive in some cases, but not all (2/5). Controls with BPH presented moderate PSMA staining and in one case also with a positive FAP staining (1/3). PET imaging with FAP seemed to result in more precise results in case of low PSMA expression than PSMA-PET.

Conclusions

While PSMA staining intensity is a valid indicator of prostate cancer in both primary tumor and lymph node metastases, the expression of FAP seems to be heterogeneous but not necessarily linked to cancer-associated fibroblasts. It is also present in inflammation-associated myofibroblasts. Therefore, its ultimate role in prostate cancer diagnosis remains a subject of discussion.
Mass spectrometry analysis of human tear fluid biomarkers specific for ocular and systemic diseases in the context of 3P medicine
Authors: Zhan Xianquan , Li Jiajia , Guo Yuna , Golubnitschaja Olga
Published: December 2021

Over the last two decades, a large number of non-communicable/chronic disorders reached an epidemic level on a global scale such as diabetes mellitus type 2, cardio-vascular disease, several types of malignancies, neurological and eye pathologies—all exerted system’s enormous socio-economic burden to primary, secondary, and tertiary healthcare. The paradigm change from reactive to predictive, preventive, and personalized medicine (3PM/PPPM) has been declared as an essential transformation of the overall healthcare approach to benefit the patient and society at large. To this end, specific biomarker panels are instrumental for a cost-effective predictive approach of individualized prevention and treatments tailored to the person. The source of biomarkers is crucial for specificity and reliability of diagnostic tests and treatment targets. Furthermore, any diagnostic approach preferentially should be noninvasive to increase availability of the biomaterial, and to decrease risks of potential complications as well as concomitant costs. These requirements are clearly fulfilled by tear fluid, which represents a precious source of biomarker panels. The well-justified principle of a “sick eye in a sick body” makes comprehensive tear fluid biomarker profiling highly relevant not only for diagnostics of eye pathologies but also for prediction, prognosis, and treatment monitoring of systemic diseases. One prominent example is the Sicca syndrome linked to a cascade of severe complications that include dry eye, neurologic, and oncologic diseases. In this review, protein profiles in tear fluid are highlighted and corresponding biomarkers are exemplified for several relevant pathologies, including dry eye disease, diabetic retinopathy, cancers, and neurological disorders. Corresponding analytical approaches such as sample pre-processing, differential proteomics, electrophoretic techniques, high-performance liquid chromatography (HPLC), enzyme-linked immuno-sorbent assay (ELISA), microarrays, and mass spectrometry (MS) methodology are detailed. Consequently, we proposed the overall strategies based on the tear fluid biomarkers application for 3P medicine practice. In the context of 3P medicine, tear fluid analytical pathways are considered to predict disease development, to target preventive measures, and to create treatment algorithms tailored to individual patient profiles.
Psychosocial Risk Factors for Overuse Injuries in Competitive Athletes: A Mixed-Studies Systematic Review
Authors: Tranaeus Ulrika , Martin Simon , Ivarsson Andreas
Published: December 2021

Background

While the psychosocial risk factors for traumatic injuries have been comprehensively investigated, less is known about psychosocial factors predisposing athletes to overuse injuries.

Objective

The aim of this review was to systematically identify studies and synthesise data that examined psychosocial risk factors for overuse injuries in athletes.

Design

Systematic review.

Data Sources

MEDLINE, Web of Science and PsycINFO databases, supplemented by hand searching of journals and reference lists.

Eligibility Criteria for Selecting Studies

Quantitative and qualitative studies involving competitive athletes, published prior to July 2021, and reporting the relationship between psychosocial variables and overuse injury as an outcome were reviewed. This was limited to academic peer-reviewed journals in Swedish, English, German, Spanish and French. An assessment of the risk of bias was performed using modified versions of the RoBANS and SBU Quality Assessment Scale for Qualitative Studies.

Results

Nine quantitative and five qualitative studies evaluating 1061 athletes and 27 psychosocial factors were included for review. Intra-personal factors, inter-personal factors and sociocultural factors were found to be related to the risk of overuse injury when synthesised and reported according to a narrative synthesis approach. Importantly, these psychosocial factors, and the potential mechanisms describing how they might contribute to overuse injury development, appeared to be different compared with those already known for traumatic injuries.

Conclusions

There is preliminary evidence that overuse injuries are likely to partially result from complex interactions between psychosocial factors. Coaches and supporting staff are encouraged to acknowledge the similarities and differences between traumatic and overuse injury aetiology.
A Preference for Peers over Faculty in the Pandemic Era: Development and Evaluation of a Medical Student-led Virtual Physiology Exam Review
Authors: Alexeeva Arina , Archibald Abigail R. , Breuer Joseph A. , Greenberg Milton L.
Published: December 2021

In the middle of the COVID-19 pandemic, students at the University of California, Irvine, reimagined their peer-led, small-group, tutorial sessions into an online format. The virtual sessions improved student-reported understanding of physiological principles and reduced exam anxiety. Peer-led review remains a valuable resource in the era of virtual medical education.
Agronomic Biofortification with Se, Zn, and Fe: An Effective Strategy to Enhance Crop Nutritional Quality and Stress Defense—A Review
Authors: Szerement Justyna , Szatanik-Kloc Alicja , Mokrzycki Jakub , Mierzwa-Hersztek Monika
Published: December 2021

Human micronutrient deficiencies are a widespread problem worldwide and mainly concern people whose diet (mainly of plant origin) consists of insufficient amounts of critical vitamins and minerals. Low levels of micronutrients in plants are linked to, i.e., their decreasing concentration in soils and/or low bioavailability and presence of abiotic stresses which disturb the proper growth and development of plants. Agronomic biofortification of crops is a very promising way to improve the concentration of micronutrients in edible parts of crops without compromising yield and is recognized as the cheapest strategy to alleviate hidden hunger worldwide. The review is focused on the factors influencing the effectiveness of biofortified crops (a type of application, form, and a dose of applied microelement, biofertilizers, and nanofertilizers). Also, the accumulation of zinc, selenium, and iron in edible parts of crops, their effects on metabolism, morphological and yield parameters, and an impact on plants’ defense mechanisms against abiotic stress like salt, high/low temperature, heavy metal, and drought was discussed. Finally, the directions of future agronomic biofortification studies are proposed.
Decolonizing Human Rights
Authors: An-Naim Abdullahi Ahmed
Published: December 2021

In his extensive body of work, Professor Abdullahi Ahmed An-Naim challenges both historical interpretations of Islamic Sharia and neo-colonial understanding of human rights. To advance the rationale of scholarship for social change, An-Naim proposes advancing the universality of human rights through internal discourse within Islamic and African societies and cross-cultural dialogue among human cultures. This book proposes a transformation from human rights organized around a state determined practice to one that is focused on a people-centric approach that empowers individuals to decide how human rights will be understood and integrated into their communities. Decolonizing Human Rights aims to illustrate the decisive role of human agency on the subject of change, without implying that Islamic or any other society are exceptionally disposed to politically motivated violence and consequent profound political instability.
INVARIANCE CRITERIA AS META-CONSTRAINTS
Authors: SAGI GIL
Published: December 2021

Invariance criteria are widely accepted as a means to demarcate the logical vocabulary of a language. In previous work, I proposed a framework of “semantic constraints” for model-theoretic consequence which does not rely on a strict distinction between logical and nonlogical terms, but rather on a range of constraints on models restricting the interpretations of terms in the language in different ways. In this paper I show how invariance criteria can be generalized so as to apply to semantic constraints on models. Some obviously unpalatable semantic constraints turn out to be invariant under isomorphisms. I shall connect the discussion to known counter-examples to invariance criteria for logical terms, and so the generalization will also shed light on the current existing debate on logicality. I analyse the failure of invariance to fulfil its role as a criterion for logicality, and argue that invariance conditions should best be thought of as merely methodological meta-constraints restricting the ways the model-theoretic apparatus should be used.
Noxious weed views and behaviors in Montana after 25 years of public education
Authors: Mangold Jane M. , Frame-Martin Shantell A. , Raile Eric D.
Published: December 2021

In 1994, a general population survey was conducted to evaluate Montanans’ knowledge about noxious weeds, and results from that survey launched an ongoing statewide education campaign. In 2019, we conducted another general population survey to assess the views and behaviors of Montanans as they relate to noxious weeds and to identify new approaches for disseminating noxious weed information. We also asked questions to evaluate changes over the 25-yr period, although our ability to make direct comparisons is subject to limitations. We implemented a mail-based survey in March through May 2019. The response rate was 18%, with 830 responses from 4,582 valid mailing addresses. Just under half (48%) of respondents report “little” or “no” knowledge about noxious weeds, which would constitute a 19-point improvement since 1994. A large majority (68%) of respondents indicate that noxious weeds are a “serious” or “very serious” problem, and appreciation for the range of negative impacts associated with noxious weeds is considerable. Most respondents (61%) identify humans as contributing “a lot” to noxious weed spread, and respondents report that their behaviors to prevent the spread of noxious weeds have increased over time. While the 1994 respondents rated television, newspaper, and radio as the best ways to disseminate information about noxious weeds, respondents now also recognize methods such as websites and social media pages as effective. Our survey identifies a need for increased educational messaging for women and people in the 18 to 39 age group. Overall, our results indicate that individuals who have seen different forms of advertisements and have participated in educational programs are more likely to consider noxious weeds a serious problem and to engage in behaviors to stop their spread.
DENDROCHRONOLOGY AND RADIOCARBON DATING
Authors: Pearson Charlotte L , Leavitt Steven W , Kromer Bernd , Solanki Sami K , Usoskin Ilya
Published: December 2021

Both dendrochronology and radiocarbon (14C) dating have their roots back in the early to mid-1900s. Although they were independently developed, they began to intertwine in the 1950s when the founder of dendrochronology, A. E. Douglass, provided dated wood samples for Willard Libby to test his emerging 14C methods. Since this early connection, absolutely dated tree-rings have been key to calibration of the Holocene portion of the 14C timescale. In turn, 14C dating of non-calendar-dated tree-rings has served to place those samples more precisely in time, advance development of long tree-ring chronologies, and bring higher resolution to earlier portions of the 14C calibration curve. Together these methods continue to shape and improve chronological frameworks across the globe, answering questions in archaeology, history, paleoclimatology, geochronology, and ocean, atmosphere, and solar sciences.
Decolonization Struggles at the United Nations: The Question of Algeria, 1955-1961
Authors: Saeed Sadia
Published: December 2021

Although national self-determination emerged as an international legal norm with the formation of the United Nations (UN) in 1945, its implementation continued to be resisted by European colonial powers for decades after. This raises the following question: how was European colonial rule challenged at the UN? This article contends that existing accounts of decolonization have not fully theorized the processes through which colonialism was contested at the UN. It fills this gap by demonstrating the critical role of argumentation, narrativization and discursive struggles through deploying the crucial “Question of Algeria” that was debated between 1955 and 1961. It demonstrates that the Algerian question yielded two opposing discourses—an anticolonial internationalist discourse and a metrocentric civilizational discourse—with both drawing on distinct ideas about human rights and development. The analysis explains the eventual triumph of the former as states increasingly rallied behind the Algerian cause.
Who Authored On Liberty? Stylometric Evidence on Harriet Taylor Mill's Contribution
Authors: Schmidt-Petri Christoph , Schefczyk Michael , Osburg Lilly
Published: December 2021

It is well known that John Stuart Mill (JSM) repeatedly acknowledges Harriet Taylor Mill's (HTM) substantial contribution to On Liberty. After her death, however, he decides to publish the book under his name only. Are we justified in continuing this practice, initiated by JSM, of refusing unequivocal co-authorship status to HTM? Drawing on stylometric analyses, we make a preliminary case that JSM did not write On Liberty all by himself and that HTM had a hand in formulating it. Drawing on plausible standards for authorship ascription, we further point out that authorship status requires, in addition to a substantial contribution, the approval by all contributors. We discuss potential reasons to assume that HTM did not approve the published version of On Liberty and would have objected to including her name on the title page.
Workforce demographics and unit structure in paediatric cardiac critical care in the United States
Authors: Horak Robin V. , Bai Shasha , Marino Bradley S. , Werho David K. , Rhodes Leslie A. , Costello John M. , Cabrera Antonio G. , Cooper David S. , Tan Yubo , Tabbutt Sarah , Krawczeski Catherine D.
Published: December 2021

Objective:

To assess current demographics and duties of physicians as well as the structure of paediatric cardiac critical care in the United States.

Design:

REDCap surveys were sent by email from May till August 2019 to medical directors (“directors”) of critical care units at the 120 United States centres submitting data to the Society of Thoracic Surgeons Congenital Heart Surgery Database and to associated faculty from centres that provided email lists. Faculty and directors were asked about personal attributes and clinical duties. Directors were additionally asked about unit structure.

Measurements and main results:

Responses were received from 66% (79/120) of directors and 62% (294/477) of contacted faculty. Seventy-six percent of directors and 54% of faculty were male, however, faculty <40 years old were predominantly women. The majority of both groups were white. Median bed count (n = 20) was similar in ICUs and multi-disciplinary paediatric ICUs. The median service expectation for one clinical full-time equivalent was 14 weeks of clinical service (interquartile range 12, 16), with the majority of programmes (86%) providing in-house attending night coverage. Work hours were high during service and non-service weeks with both directors (37%) and faculty (45%).

Conclusions:

Racial and ethnic diversity is markedly deficient in the paediatric cardiac critical care workforce. Although the majority of faculty are male, females make up the majority of the workforce younger than 40 years old. Work hours across all age groups and unit types are high both on- and off-service, with most units providing attending in-house night coverage.
Universal Credit and Automated Decision Making: A Case of the Digital Tail Wagging the Policy Dog?
Authors: Griffiths Rita
Published: December 2021

Intended to simplify the benefit system and ’make work pay’, Universal Credit (UC) is the UK’s first ‘digital by design’ benefit. Proponents of UC highlight the greater efficiency and effectiveness of digitalisation, while critics point to costly IT write-offs and the ‘digital divide’ between people with the skills and resources to access digital technologies, and those without. Less attention has been paid to automation in UC and its effects on the people subject to these rapidly developing technologies. Findings from research exploring couples’ experiences of claiming UC suggest that automated processes for assessing entitlement and calculating payment may be creating additional administrative burdens for some claimants. Rigid design parameters built into UC’s digital architecture may also restrict options for policy reform. The article calls for a broadening of thinking and research about digitalisation in welfare systems to include questions of administrative burden and the wider effects and impacts on claimants.
Using MERSCOPE to Generate a Cell Atlas of the Mouse Brain that Includes Lowly Expressed Genes
Authors: Emanuel George , He Jiang
Published: December 2021

The structure and organization of cells within organs is essential to their function, but nowhere in the body is this more spectacular than the brain. There, sprawling, snowflake-like neurons have grown into a precise arrangement, reaching out to neighboring cells to form neural circuits. Communication within neural circuits, made possible by spatial positioning, forms the basis of our physiology. Recently, a high-resolution cell atlas generated by MERFISH (multiplex error-robust fluorescence in situ hybridization) technology has mapped this spectacular organ with unmatched resolution, depth, and scale. The atlas catalogs cells as they exist in the intact biological system and will allow us to learn more about rare cell types and sparsely expressed cell signaling receptors fundamental to health and disease.
The Civilizing Mission Persists
Authors: Gill Timothy M.
Published: December 2021

U.S. government leaders have long considered Latin America their proverbial backyard and have recurrently intervened in the region. In earlier periods of U.S. imperialism, U.S. government leaders justified such intervention with reference to allegedly scientific racial hierarchies, which placed White, Anglo-Saxon Protestants (WASPs) at the top of this artificial hierarchy. In more recent episodes of U.S. imperialism leading into the twenty-first century, however, U.S. leaders have publicly used the language of democracy and human rights to justify intervention. In the instance of contemporary Venezuela, while U.S leaders indeed use the language of human rights and democracy, they also draw on racist tropes of Latin Americans to justify their intervention. Through interviews with U.S. foreign policymakers and analysis of U.S. government documents, I find that U.S. leaders depict former Venezuelan President Hugo Chávez as an irrational, uncivilized, and beastly leader, who manipulates ideas of racial inequality to maintain power. In addition, U.S. leaders understand him as manipulating an uncritical mass of Venezuelans who cannot think for themselves. U.S. leaders believe it thus their duty to intervene in order to promote democracy and show Venezuelans their true political-economic interests. I connect these dynamics with a history of U.S. intervention into the region and a history of racist and imperial thinking that continues to shape the logic of U.S. foreign policymaking into the present.
SZEMERÉDI’S THEOREM: AN EXPLORATION OF IMPURITY, EXPLANATION, AND CONTENT
Authors: RYAN PATRICK J.
Published: December 2021

In this paper I argue for an association between impurity and explanatory power in contemporary mathematics. This proposal is defended against the ancient and influential idea thatpurityand explanation go hand-in-hand (Aristotle, Bolzano) and recent suggestions that purity/impurity ascriptions and explanatory power are more or less distinct (Section 1). This is done by analyzing a central and deep result of additive number theory, Szemerédi’s theorem, and various of its proofs (Section 2). In particular, I focus upon the radically impure (ergodic) proof due to Furstenberg (Section 3). Furstenberg’s ergodic proof is striking because it utilizes intuitively foreign and infinitary resources to prove a finitary combinatorial result and does so in a perspicuous fashion. I claim that Furstenberg’s proof is explanatory in light of its clear expression of a crucial structural result, which provides the “reason why” Szemerédi’s theorem is true. This is, however, rather surprising: how can such intuitively different conceptual resources “get a grip on” the theorem to be proved? I account for this phenomenon by articulating a new construal of the content of a mathematical statement, which I callstructural content(Section 4). I argue that the availability of structural content saves intuitive epistemic distinctions made in mathematical practice and simultaneously explicates the intervention of surprising and explanatorily rich conceptual resources. Structural content also disarms general arguments for thinking that impurity and explanatory power might come apart. Finally, I sketch a proposal that, once structural content is in hand, impure resources lead to explanatory proofs via suitably understood varieties of simplification and unification (Section 5).
First study of the bat fossil record of the mid-Atlantic volcanic islands
Authors: GONZÁLEZ-DIONIS Javier , CASTILLO RUIZ Carolina , CRUZADO-CABALLERO Penélope , CADAVID-MELERO Elena , CRESPO Vicente D.
Published: December 2021

Bats are one of the most abundant and important mammals in ecosystems. However, their fossil record is scarce and fragile, making them difficult to find. Accordingly, there is no record of this group in the volcanic islands of the mid-Atlantic Ocean apart from the Canary Islands. This paper studies the first bat fossil record of the Canary Islands (Spain). The material studied is found within two Quaternary lava tubes, Cueva de los Verdes on Lanzarote and Cueva Roja on the island of El Hierro. The dental and humeral morphology and biometry are analysed and compared with current specimens. Among our results we highlight the first fossil data of two species endemic to the islands of the mid-Atlantic Ocean,Plecotus teneriffaeandPipistrellus maderensis, the former from the Canary Islands and the latter from the Azores, Madeira and the Canary Islands. We also confirm the presence ofPipistrellus kuhliiin the fossil record of the island of Lanzarote. No differences are observed between the dental morphology of the current and the fossil populations ofP. maderensisandPl. teneriffae. In the case ofP. kuhlii, the populations of the Canary Islands and the Iberian Peninsula show differences in the paraconule with respect to the populations from central Europe. Palaeoecological studies of these taxa suggest that these islands presented a similar habitat when the sites were formed to the present-day habitat.
The relationship between international humanitarian law and asset freeze obligations under United Nations sanctions
Authors: Onishi Kosuke
Published: December 2021

While challenges may persist with respect to the relationship between counterterrorism (CT) and humanitarian action, it is at least understood that CT measures must comply with international humanitarian law (IHL). Clarifying the relationship between this body of law and CT measures is one of the modest but important innovations of United Nations (UN) Security Council Resolution 2462. At a minimum, references to IHL in this resolution leave a pathway for States to take measures to preserve impartial humanitarian action from the effects of CT, and at most, they prescribe that States should take such measures. Progress in clarifying the relationship between UN sanctions obligations and IHL obligations appears to be lacking with respect to non-CT-related UN sanctions. As will be discussed in this paper, this leads to questions regarding the application of the so-called “supremacy clause” contained in Article 103 of the UN Charter vis-à-vis IHL obligations.
A systematic review of cardiovascular risk factors in patients with traumatic spinal cord injury
Authors: Wahl Uwe , Hirsch Tobias
Published: December 2021

Summary: Objective: The main risk factors for cardiac events, and particularly for the development of atherosclerosis, are diabetes mellitus, arterial hypertension, dyslipidemia and smoking. Patients with a traumatic spinal cord injury (SCI) may present with autonomic nervous system dysfunction depending on their level of spinal cord injury. Studies have found a rise in cardiovascular mortality. A systematic review was conducted that focused on this patient group’s predisposition to vascular risk. Methods: We performed a PubMed and Cochrane database search. After applying specific search criteria, 42 articles were included in our analysis out of a total of 10,784 matches. The articles were selected with the aim of establishing cardiovascular risk factors in patients with traumatic spinal cord injury. Results: Patients with SCI are at an increased risk for peripheral artery disease even in the absence of cardiovascular risk factors. Major vascular changes to the arteries of patients with SCI include: a reduction in lumen size, increased vessel wall tension, higher vascular stiffness, an impaired reactive hyperemic response, and a lack of reduced vascular resistance. The findings for carotid atherosclerosis were inconclusive. This group of patients also has a higher disposition for diabetes mellitus, lipid metabolism disorders and coronary artery disease. Paraplegics are more likely to suffer from dyslipidemia, obesity and PAD, while tetraplegics are more likely to have diabetes mellitus. Conclusions: Patients with SCI are more likely to have cardiovascular risk factors and have cardiovascular disease compared to the normal population. Peripheral circulatory disorders are particularly common. Patients with SCI are now considered to be a new risk group for cardiovascular disease; however, large epidemiological studies are needed to verify in more detail the cardiovascular risk profile of this patient group.
The Far-Reaching Consequences of Wildfire Smoke Plumes
Authors: Wheeling Kate
Published: December 2021

Smoke from wildfires burning in the western United States carries harmful pollutants across the country.
Three‐Dimensional Free Gas Flow Focuses Basin‐Wide Microbial Methane to Concentrated Methane Hydrate Reservoirs in Geological System
Authors: You Kehua , Summa Lori , Flemings Peter , Santra Manasij , Fang Yi
Published: December 2021

We present a systematic model that links the generation, migration, phase partitioning, and accumulation of methane into a closed loop as the sediment is deposited from the seafloor and buried through the base of hydrate stability zone (BHSZ). In our model, methane is generated by biodegradation of organic carbon in muds. Hydrate does not form and methane is not trapped until a coarse‐grained layer is deposited, because the small pores prevent hydrate formation in muds. Instead, methane diffuses into sands/silts where methane solidifies into hydrate. As hydrate‐bearing sands/silts pass through the BHSZ during sediment burial, methane hydrate dissociates, and releases free gas. The released and the newly generated free gas below the BHSZ concentrates into a vertical/dipping zone with low capillary entry pressure and high permeability and flows upward driven its buoyancy. When free gas reaches the hydrate stability zone (HSZ), capillary forces drive free gas to flow laterally, preferentially enter sands/silts, feed hydrate growth, and elevate hydrate saturation. With three‐dimensional focused free gas flow, microbial methane that is generated from a much larger fetch area of the entire basin, both above and below the BHSZ, is concentrated into coarse‐grained layers at structural closures for hydrate formation. Our model illustrates how geological evolution, microbial methane generation, and gas flow by buoyancy couple to generate concentrated hydrate deposits in geological system. These insights can be used to explore for high‐concentration methane hydrate and are important for understanding the methane budget and carbon cycle under the seafloor.
A Two‐Stage Bayesian Data‐Driven Method to Improve Model Prediction
Authors: Sun Xiaozhuo , Zeng Xiankui , Wu Jichun , Wang Dong
Published: December 2021

Because of the earth system complexity, groundwater/hydrology models are always built with structural errors, which may lead to systematic errors in model predictions. Bayesian data‐driven methods (DDMs) provide a feasible way to correct systematic model errors statistically. Generally, the physical and statistical model parameters, namely physical parameters and hyperparameters, are assumed to be independent and jointly calibrated. However, this assumption may be unreasonable and lead to over‐adjusted parameter estimation and biased model prediction. This study proposes a two‐stage DDM to calibrate physical parameters and hyperparameters separately, which does not make the independence assumption. Three case studies, including a groundwater solute transport analytical model, a three‐dimensional groundwater flow model, and a real‐world snowmelt runoff model, were used to evaluate the predictive performance of this two‐stage DDM. Based on the three case studies, we found that the independence assumption of physical parameters and hyperparameters could lead to the over‐fitting of parameter estimation and deviations in model predictions. Two‐stage DDM can constrain the systematic error model calibration; that is, physical parameters are first calibrated in the entire hyperparameter prior probability space, and then hyperparameters are calibrated in the posterior probability space of physical parameters obtained previously. As a result, compared with traditional joint calibration‐based DDM, two‐stage DDM can alleviate parameter over‐fitting and improve model predictive performance.
Amino acid deprivation induces AKT activation by inducing GCN2/ATF4/REDD1 axis
Authors: Jin Hyeon-Ok , Hong Sung-Eun , Kim Ji-Young , Jang Se-Kyeong , Park In-Chul
Published: December 2021

Amino acid availability is sensed by various signaling molecules, including general control nonderepressible 2 (GCN2) and mechanistic target of rapamycin complex 1 (mTORC1). However, it is unclear how these sensors are associated with cancer cell survival under low amino acid availability. In the present study, we investigated AKT activation in non-small cell lung cancer (NSCLC) cells deprived of each one of 20 amino acids. Among the 20 amino acids, deprivation of glutamine, arginine, methionine, and lysine induced AKT activation. AKT activation was induced by GCN2/ATF4/REDD1 axis-mediated mTORC2 activation under amino acid deprivation. In CRISPR-Cas9-mediated REDD1-knockout cells, AKT activation was not induced by amino acid deprivation, indicating that REDD1 plays a major role in AKT activation under amino acid deprivation. Knockout of REDD1 sensitized cells cultured under glutamine deprivation conditions to radiotherapy. Taken together, GCN2/ATF4/REDD1 axis induced by amino acid deprivation promotes cell survival signal, which might be a potential target for cancer therapy.
Reducing the nanoparticles generated at the wheel–rail contact by applying tap water lubricant at subway train operational velocities
Authors: Lee HyunWook
Published: December 2021

The formation characteristics and the reduction of nanoparticles emitted from wheel–rail contacts at subway-train velocities of 73, 90, and 113 km/h under dry and water-lubricated conditions (using tap water) were studied using a twin-disk rig. The resulting number concentration (NC) of ultrafine and fine particles increased with train velocity under both conditions. Particle generation varied with slip rate under both conditions in both the particle categories. Furthermore, the formation characteristics at 113 km/h under dry conditions showed a notable deviation from those under water-lubricated conditions in three aspects: (i) The maximum NC of ultrafine particles was higher than that of fine particles, (ii) the predominant peak diameter was in the ultrafine particles category, and (iii) the proportion of ultrafine particles was much higher than those of the fine particles. Applying water decreased the NC of ultrafine and fine particles significantly at all tested velocities (by 54–69% and 87–91%, respectively). Adding water increased the NC of particles ≤ 35 nm in diameter, possibly owing to the increase in water vapor and mineral crystals from tap water. Overall, this study provides a reference for researchers aiming to minimize nanoparticle formation at the wheel–rail contacts by applying a lubricant.
Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
Authors: Rivai Reza Ramdan , Miyamoto Takuji , Awano Tatsuya , Takada Rie , Tobimatsu Yuki , Umezawa Toshiaki , Kobayashi Masaru
Published: December 2021

Sorghum [Sorghum bicolor(L.) Moench] has been gaining attention as a feedstock for biomass energy production. While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components. Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls. The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods. We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings. Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-d-glucan, and arabinoxylan. At least some of these cell wall alterations could be associated with changes in gene expression. Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.
Influenza virus vaccination in pediatric nephrotic syndrome significantly reduces rate of relapse and influenza virus infection as assessed in a nationwide survey
Authors: Ishimori Shingo , Ando Takashi , Kikunaga Kaori , Terano Chikako , Sato Mai , Komaki Fumiyo , Hamada Riku , Hamasaki Yuko , Araki Yoshinori , Gotoh Yoshimitsu , Nakanishi Koichi , Nakazato Hitoshi , Matsuyama Takeshi , Iijima Kazumoto , Yoshikawa Norishige , Ito Shuichi , Honda Masataka , Ishikura Kenji
Published: December 2021

Although vaccination may precipitate relapses of nephrotic syndrome (NS) in children with idiopathic NS, no data are available regarding NS activity regarding influenza (flu) virus infections and NS relapses after receiving inactivated flu vaccines. We conducted a nationwide study of children aged 6 months to 15 years with idiopathic NS to assess the relationship between NS relapse, flu vaccination, and flu infections. We used a multivariate Poisson regression model (MPRM) to calculate the risk ratio (RR) for flu infection and for NS relapse in children with and without flu vaccination. Data of 306 children were assessed. The MPRM in all 306 children showed a significantly lower RR for flu infection (RR: 0.21, 95% confidence interval CI 0.11–0.38) and for NS relapse (RR: 0.22, 95% CI 0.14–0.35) in children receiving flu vaccination compared with unvaccinated children. In an additional MPRM only among 102 children receiving flu vaccination, they had a significantly lower risk for NS relapse during the post-vaccination period (RR: 0.31. 95% CI 017–0.56) compared with the pre-vaccination period. Although our study was observational, based on the favorable results of flu vaccinations regarding flu infections and NS relapse, the vaccine may be recommended for children with NS.
Ureteral stone with hydronephrosis and urolithiasis alone are risk factors for acute kidney injury in patients with urinary tract infection
Authors: Hsiao Chih-Yen , Chen Tsung-Hsien , Lee Yi-Chien , Wang Ming-Cheng
Published: December 2021

To identify whether urolithiasis with or without hydronephrosis has an impact on acute kidney injury (AKI) in patients with urinary tract infection (UTI). This study aimed to identify whether urolithiasis with or without hydronephrosis has an impact on AKI in patients with UTI. This retrospective study enrolled hospitalized UTI patients who underwent imaging in an acute care setting from January 2006 to April 2019. Of the 1113 participants enrolled, 191 (17.2%) had urolithiasis and 76 (6.8%) had ureteral stone complicated with hydronephrosis. Multivariate logistic regression analysis showed that in UTI patients with urolithiasis, the presence of ureteral stone with concomitant hydronephrosis was an independent risk factor for AKI (odds ratio [OR] 2.299, 95% confidence interval [CI] 1.112–4.755, P = 0.025). In addition, urolithiasis was associated with an increased risk for AKI (OR 2.451, 95% CI 1.369–4.389, P = 0.003) in UTI patients without hydronephrosis. The presence of ureteral stone with hydronephrosis increases the risk for AKI of UTI patients with urolithiasis, and urolithiasis remains a risk factor of AKI in UTI patients without hydronephrosis.
Diet-driven mercury contamination is associated with polar bear gut microbiota
Authors: Watson Sophie E. , McKinney Melissa A. , Pindo Massimo , Bull Matthew J. , Atwood Todd C. , Hauffe Heidi C. , Perkins Sarah E.
Published: December 2021

The gut microbiota may modulate the disposition and toxicity of environmental contaminants within a host but, conversely, contaminants may also impact gut bacteria. Such contaminant-gut microbial connections, which could lead to alteration of host health, remain poorly known and are rarely studied in free-ranging wildlife. The polar bear (Ursus maritimus) is a long-lived, wide-ranging apex predator that feeds on a variety of high trophic position seal and cetacean species and, as such, is exposed to among the highest levels of biomagnifying contaminants of all Arctic species. Here, we investigate associations between mercury (THg; a key Arctic contaminant), diet, and the diversity and composition of the gut microbiota of polar bears inhabiting the southern Beaufort Sea, while accounting for host sex, age class and body condition. Bacterial diversity was negatively associated with seal consumption and mercury, a pattern seen for both Shannon and Inverse Simpson alpha diversity indices (adjusted R2 = 0.35, F1,18 = 8.00, P = 0.013 and adjusted R2 = 0.26, F1,18 = 6.04, P = 0.027, respectively). No association was found with sex, age class or body condition of polar bears. Bacteria known to either be involved in THg methylation or considered to be highly contaminant resistant, including Lactobacillales, Bacillales and Aeromonadales, were significantly more abundant in individuals that had higher THg concentrations. Conversely, individuals with higher THg concentrations showed a significantly lower abundance of Bacteroidales, a bacterial order that typically plays an important role in supporting host immune function by stimulating intraepithelial lymphocytes within the epithelial barrier. These associations between diet-acquired mercury and microbiota illustrate a potentially overlooked outcome of mercury accumulation in polar bears.
Associations between parent–child relationship, and children’s externalizing and internalizing symptoms, and lifestyle behaviors in China during the COVID-19 epidemic
Authors: Du Fanxing , He Li , Francis Mark R. , Forshaw Mark , Woolfall Kerry , Lv Qian , Shi Lu , Hou Zhiyuan
Published: December 2021

To investigate associations between parent–child relationships, children’s externalizing and internalizing symptoms, and lifestyle responses to the COVID-19 epidemic, we conducted an online survey of a random, representative sample of residents with children aged 3–17 years during mid-March 2020 in Wuhan and Shanghai, China. A total of 1655 parents and children were surveyed with a response rate of 80.1% in the survey. During the epidemic, the frequency of children enquiring about the epidemic (AOR = 1.46, 95% CI: 1.04, 2.06), parents explaining the epidemic to them (AOR = 2.87, 95% CI: 1.80, 4.58), parents expressing negative emotions in front of them (AOR = 2.62; 95% CI = 2.08–3.30), and parents with more irritable attitudes (AOR = 1.93; 95% CI = 1.33–2.81) were significantly associated with children’s externalizing symptoms. For internalizing symptoms, significant associations were found with worse parent–child closeness (AOR = 2.93; 95% CI = 1.80–4.79), the frequency of parents expressing negative emotions in front of them (AOR = 2.64; 95% CI = 1.68, 4.12), and more irritable attitudes (AOR = 2.24; 95% CI = 1.42–3.55). We also found that each indicator of parent–child relationships had the significantly similar associations with children’s lifestyle behaviors. These findings suggest that improving parents’ attitudes towards their children and parent–child closeness during the epidemic, especially among parents with lower educational levels, are important to ensure the wellbeing of children.
Integrated RNAi screening identifies the NEDDylation pathway as a synergistic partner of azacytidine in acute myeloid leukemia
Authors: Klosner Justine , Agelopoulos Konstantin , Rohde Christian , Göllner Stefanie , Schliemann Christoph , Berdel Wolfgang E. , Müller-Tidow Carsten
Published: December 2021

Treatment of acute myeloid leukemia (AML) remains challenging and novel targets and synergistic therapies still need to be discovered. We performed a high-throughput RNAi screen in three different AML cell lines and primary human leukemic blasts to identify genes that synergize with common antileukemic therapies. We used a pooled shRNA library that covered 5043 different genes and combined transfection with exposure to either azacytidine or cytarabine analog to the concept of synthetic lethality. Suppression of the chemokine CXCL12 ranked highly among the candidates of the cytarabine group. Azacytidine in combination with suppression of genes within the neddylation pathway led to synergistic results. NEDD8 and RBX1 inhibition by the small molecule inhibitor pevonedistat inhibited leukemia cell growth. These findings establish an in vitro synergism between NEDD8 inhibition and azacytidine in AML. Taken together, neddylation constitutes a suitable target pathway for azacytidine combination strategies.