bluuebunny/crossref_metadata_2025 huggingface dataset:
Magnetic-Resonance-Imaging-Based Left Atrial Strain and Left Atrial Strain Rate as Diagnostic Parameters in Cardiac Amyloidosis
Authors: Sciacca Vanessa , Eckstein Jan , Körperich Hermann , Fink Thomas , Bergau Leonard , El Hamriti Mustapha , Imnadze Guram , Guckel Denise , Fox Henrik , Gerçek Muhammed , Farr Martin , Burchert Wolfgang , Sommer Philipp , Sohns Christian , Piran Misagh
Published: June 2022

Aims: The present study aims to evaluate magnetic-resonance-imaging (MRI)-assessed left atrial strain (LAS) and left atrial strain rate (LASR) as potential parameters for the diagnosis of cardiac amyloidosis (CA), the distinction of clinical subtypes and differentiation from other cardiomyopathies. Methods and results: LAS and LASR were assessed by MRI feature tracking in patients with biopsy-proven CA. LAS and LASR of patients with CA were compared to healthy subjects and patients with hypertrophic cardiomyopathy. LAS and LASR were also analyzed concerning differences between patients with transthyretin (ATTR) and light chain amyloidosis (AL). A total of 44 patients with biopsy-proven CA, 19 patients with hypertrophic cardiomyopathy and 24 healthy subjects were included. In 22 CA patients (50%), histological examination identified ATTR as CA subtype and AL in the remaining patients. No significant difference was observed for reservoir, conduit or booster LAS in patients with AL or ATTR. Reservoir LAS, conduit LAS and booster LAS were significantly reduced in patients with CA and HCM as compared to healthy subjects (p < 0.001). Reservoir LAS and booster LAS were significantly reduced in CA as compared to HCM patients (p < 0.001). A linear correlation was observed between LA global reservoir strain and LA-EF (p < 0.001, r = 0.5), conduit strain and global longitudinal LV strain (p < 0.001, r = 0.5), global booster strain rate and LA-EF (p < 0.001, r = 0.6) and between global booster strain rate and LA area at LVED (p < 0.0001, 0.5). Conclusions: LAS and LASR are severely impaired in patients with CA. The MRI-based assessment of LAS and LASR might allow non-invasive diagnosis and categorization of CA and its distinct differentiation from other hypertrophic phenotypes.
Development of Histologically Verified Thyroid Diseases in Women Operated for Breast Cancer: A Review of the Literature and a Case Series
Authors: Fama’ Fausto , Sindoni Alessandro , Sun Hui , Kim Hoon Yub , Geraci Girolamo , Colonna Michele Rosario , Mazzeo Carmelo , Brenta Gabriela , Galeano Mariarosaria , Benvenga Salvatore , Dionigi Gianlorenzo
Published: June 2022

Background: The possible relationships between breast and thyroid diseases have been reported in the literature. The purpose of our study was to evaluate the occurrence of histologically verified thyroid pathologies in women who were diagnosed with breast cancer and, after mastectomy/quadrantectomy complemented by oncological treatment, were thyroidectomized based on their periodic thyroid evaluation. Patients and Methods: Our series consist of 31 women with a mean age of 62.9 ± 10.9 years (range, 45–81) treated for breast cancer (18 right-sided, 11 left-sided, and 2 bilateral), of whom 29 were thyroidectomized, since two women who developed Graves’ disease refused thyroidectomy. These 31 women belong to a cohort of 889 women who referred to the Breast Surgery Unit of our university hospital during the period January 2010 through December 2020. Results: The mean time interval between breast cancer and thyroid pathologies was 48.1 ± 23.4 months (range, 12–95). The final diagnosis at histopathology was infiltrating ductal breast carcinoma in 26 women (with 2/26 patients having bilateral carcinoma) and infiltrating lobular breast carcinoma in the other 5 women. Ten of the twenty-nine thyroidectomized women (34.5%) had a thyroid malignancy on histology: five papillary carcinomas, three papillary micro-carcinomas and two follicular carcinomas. Two of the five women with papillary carcinoma also had histological evidence of chronic lymphocytic thyroiditis/Hashimoto’s thyroiditis, which was also detected in another five women with benign thyroid diseases. Conclusions: We suggest that breast cancer survivors should be made aware of the possible increased risk of thyroid pathologies (including thyroid malignancy) so that they can undergo screening and follow-up.
Paravalvular Leak Echo Imaging before and during the Percutaneous Procedure
Authors: Pysz Piotr , Wojakowski Wojtek , Smolka Grzegorz
Published: June 2022

Percutaneous device closure has become a valuable alternative to surgery in the management of paravalvular leaks. Consequently, imaging in these patients is currently not only meant to verify the hemodynamic significance of the lesion but also to assess the feasibility of transcatheter treatment. We present a methodology of comprehensive echocardiography assessment that allows for the selection of patients and plans the intervention. Next, procedure-oriented steps of echocardiography imaging, which are essential for eventual success, are reviewed.
Crossing Phylums: Butterfly Wing as a Natural Perfusable Three-Dimensional (3D) Bioconstruct for Bone Tissue Engineering
Authors: Mostofi Fatemeh , Mostofi Marzieh , Niroomand Behnaz , Hosseini Saadi , Alipour Atefeh , Homaeigohar Shahin , Mohammadi Javad , Shokrgozar Mohammad Ali , Shahsavarani Hosein
Published: June 2022

Despite the advent of promising technologies in tissue engineering, finding a biomimetic 3D bio-construct capable of enhancing cell attachment, maintenance, and function is still a challenge in producing tailorable scaffolds for bone regeneration. Here, osteostimulatory effects of the butterfly wings as a naturally porous and non-toxic chitinous scaffold on mesenchymal stromal cells are assessed. The topographical characterization of the butterfly wings implied their ability to mimic bone tissue microenvironment, whereas their regenerative potential was validated after a 14-day cell culture. In vivo analysis showed that the scaffold induced no major inflammatory response in Wistar rats. Topographical features of the bioconstruct upregulated the osteogenic genes, including COL1A1, ALP, BGLAP, SPP1, SP7, and AML3 in differentiated cells compared to the cells cultured in the culture plate. However, butterfly wings were shown to provide a biomimetic microstructure and proper bone regenerative capacity through a unique combination of various structural and material properties. Therefore, this novel platform can be confidently recommended for bone tissue engineering applications.
Microcosmic Characteristics of Hydrate Formation and Decomposition in the Different Particle Size Sediments Captured by Cryo-SEM
Authors: Lu Jingsheng , Lin Decai , Li Dongliang , Liang Deqing , Wen Long , Wu Siting , Zhang Yiqun , He Yong , Shi Lingli , Xiong Youming
Published: June 2022

Sand production and sand control studies in non-diagenetic reservoirs are the weak point in the conventional petroleum industry. However, natural gas hydrate (NGH) mainly exists in non-diagenetic strata, and sand production occurs during exploitation, which restricts the safe and sustainable production of NGH. To study the microcosmic characteristics of sand production, the hydrate decomposition behaviours in the sediments were captured by the Cryo-SEM method. The micromorphology of different particle sizes of sand samples containing NGH (the sand median diameter d50 is 150, 87, 55, 38, 24, and 13 µm) and the microcosmic processes of NGH decomposition were observed. Then, the microcosmic characteristics of sand production, during the decomposition process, were analysed. (1) The gas hydrate decomposition increases pore space and reduces reservoir strength; the expansion action of the decomposition of water and gas, the softening action of the decomposition of water, and the compression action by overlying stress (crustal stress) promoted sand production, deformation, and subsidence of the NGH reservoirs in the mining process. (2) The decomposition of NGH has a more significant impact on sediments with smaller particle sizes. (3) The particle size of NGH may be larger than the particle size of the mud in the reservoirs, and acting as “gravel” plays a particular role in sand control. Therefore, the particle size of NGH cannot be ignored in the design of sand control. (4) It has been revealed, and verified, that sputtering (splashing) is a unique process of sand production caused by NGH decomposition. In other words, the rapid expansion of the volume of the decomposed gas and water from NGH leads to the eruption and sputtering of hydrate particles, providing the driving force for sand migration, which is a different process of sand production than in conventional oil and gas.
Special Issue “Precision Medicine in Neurodevelopmental Disorders: Personalized Characterization of Autism from Molecules to Behavior”
Authors: Torres Elizabeth B.
Published: June 2022

The Precision Medicine (PM) platform [...]
Multivariate Analysis of Metabolomic and Nutritional Profiles among Children with Autism Spectrum Disorder
Authors: Qureshi Fatir , Adams James B. , Audhya Tapan , Hahn Juergen
Published: June 2022

There have been promising results regarding the capability of statistical and machine-learning techniques to offer insight into unique metabolomic patterns observed in ASD. This work re-examines a comparative study contrasting metabolomic and nutrient measurements of children with ASD (n = 55) against their typically developing (TD) peers (n = 44) through a multivariate statistical lens. Hypothesis testing, receiver characteristic curve assessment, and correlation analysis were consistent with prior work and served to underscore prominent areas where metabolomic and nutritional profiles between the groups diverged. Improved univariate analysis revealed 46 nutritional/metabolic differences that were significantly different between ASD and TD groups, with individual areas under the receiver operator curve (AUROC) scores of 0.6–0.9. Many of the significant measurements had correlations with many others, forming two integrated networks of interrelated metabolic differences in ASD. The TD group had 189 significant correlation pairs between metabolites, vs. only 106 for the ASD group, calling attention to underlying differences in metabolic processes. Furthermore, multivariate techniques identified potential biomarker panels with up to six metabolites that were able to attain a predictive accuracy of up to 98% for discriminating between ASD and TD, following cross-validation. Assessing all optimized multivariate models demonstrated concordance with prior physiological pathways identified in the literature, with some of the most important metabolites for discriminating ASD and TD being sulfate, the transsulfuration pathway, uridine (methylation biomarker), and beta-amino isobutyrate (regulator of carbohydrate and lipid metabolism).
An NLP Approach for Extracting Practical Knowledge from a CMS-Based Community of Practice in E-Learning
Authors: Leung Javier
Published: June 2022

This study aimed to identify the tacit or practical knowledge of an online community of practice (CoP) based on a content management system (CMS) technology. The E-Learning Industry site is one of the most prominent news outlets that provides instructional design and technology (IDT) practitioners with insights into the field. Natural language processing (NLP) techniques were implemented to extract practical knowledge of publicly available and not password-protected text sources in seven news categories. First, the findings suggest emphasizing the production of online articles related to the production of e-learning materials in technology-enabled environments. Second, the results indicate the alternative uses of learning management systems to manage different aspects of the production of e-learning materials. Third, the findings show that the CoP’s main priority was to reference existing materials in the community and external resources. The results of this study have implications and provide recommendations for researchers, community leaders, and practitioners toward improving knowledge discovery mechanisms, increasing transparency and integrity in communities, and increasing practitioners’ ability to self-assess existing practical knowledge against competencies in the field. The present study takes an inventory of the organizational knowledge capital and functions embedded in a CoP using a CMS platform as a delivery mechanism for creating and sharing knowledge.
Centennial-Scale Land-Cover Change on Babeldaob Island, Palau
Authors: Dendy Julian , Collins Paul , Mesubed Dino , Cordell Susan , Giardina Christian P. , Uowolo Amanda , Iida Akiko
Published: June 2022

We used publicly available land-cover datasets (1921, 1947, 1976, 1992, 2001, 2006, 2014) to assess land-cover change on Babeldaob Island, Republic of Palau. Land-cover data were organized, classified, and summarized by area and percentage of island cover for forest, mangrove, non-forest vegetation, and non-vegetation. Japanese colonial administration of the island between 1921 and 1947 included industrial mining and agriculture, which reduced total non-mangrove forest cover by 16%. Temporal vegetation cover dynamics from 1947 through 2014 indicate unassisted forest regeneration of 12% with a concomitant decrease in non-forest vegetation including abandoned agriculture, the majority of which happened by 1976. Mangrove cover appears to have remained stable throughout the 93 year study period. Ground-truth observations showed the highest rates of vegetation cover change since 1976 for non-vegetated areas, followed by marsh and swamp forest. The proportion of non-vegetated cover increased at least twofold after WWII, with at least half comprising roads. Contrary to other tropical geographies, we saw forest expansion following completion of a major perimeter road. However, the larger landscape is permeated by dynamic forest edges subject to wildfire and other land-use disturbances.
The Incidence and Associated Risk Factors of Contrast-Induced Nephropathy after Contrast-Enhanced Computed Tomography in the Emergency Setting: A Systematic Review
Authors: Ong Mei-Yi , Koh Justin Jie-Hui , Kothan Suchart , Lai Christopher
Published: June 2022

Iodinated contrast media (ICM) during contrast-enhanced computed tomography (CECT) in the emergency department (ED) is essential to diagnose acute conditions, despite risks of contrast-induced nephropathy (CIN) development and its associated complications. This systematic review aims to evaluate the incidence of CIN and CIN-induced complications, and to explore the relevance of classical risk factors for CIN among ED patients receiving ICM. PubMed, Cochrane, and Web of Science were used on 30 August 2021 to search for peer-reviewed English articles reporting on CIN incidence among ED patients aged ≥18 years who underwent an intravenous CECT. The inclusion criteria included studies that were in English, peer-reviewed, and involved ED patients aged ≥18 years who underwent single intravenous CECT. Studies on intra-arterial procedures and preventive strategies, meta-analyses, clinical guidelines, review articles, and case reports were excluded. The JBI critical appraisal checklist was applied to assess the risk of bias. In total, 18 studies were included wherein 15 were retrospective studies while three were prospective studies. We found a relatively higher CIN incidence in the ED, with variations owing to the CIN definitions. Several classical risk factors including acute hypotension remain linked to CIN onset in ED settings unlike factors such as age and diabetes. While risk of adverse renal events due to CIN is low, there is higher risk of CIN-induced mortality in the ED. Therefore, with the higher incidence of CIN and CIN-induced mortality rates in the ED, ICM administration during CECT in the ED should still be clinically justified after assessing both benefits and risks.
Intrinsic Properties of Multi-Layer TiO2/V2O5/TiO2 Coatings Prepared via E-Beam Evaporation
Authors: Rehman Irfa , Hanif Muhammad Bilal , Alghamdi Abdulaziz Salem , Khaliq Abdul , Halim K. S. Abdel , Subhani Tayyab , Motola Martin , Khan Abdul Faheem
Published: June 2022

Nanocomposite multi-layer TiO2/V2O5/TiO2 thin films were prepared via electron-beam evaporation using high-purity targets (TiO2 and V2O5 purity > 99.9%) at substrate temperatures of 270 °C (TiO2) and 25 °C (V2O5) under a partial pressure of oxygen of 2 × 10−4 mbar to maintain the stoichiometry. Rutherford backscattering spectrometry was used to confirm the layer structure and the optimal stoichiometry of the thin films, with a particle size of 20 to 40 nm. The thin films showed an optical transmittance of ~78% in the visible region and a reflectance of ~90% in the infrared. A decrease in transmittance was observed due to the greater cumulative thickness of the three layers and multiple reflections at the interface of the layers. The optical bandgap of the TiO2 mono-layer was ~3.49 eV, whereas that of the multi-layer TiO2/V2O5/TiO2 reached ~3.51 eV. The increase in the optical bandgap was due to the inter-diffusion of the layers at an elevated substrate temperature during the deposition. The intrinsic, structural, and morphological features of the TiO2/V2O5/TiO2 thin films suggest their efficient use as a solar water heater system.
Compressive Strength Prediction of Rubber Concrete Based on Artificial Neural Network Model with Hybrid Particle Swarm Optimization Algorithm
Authors: Huang Xiao-Yu , Wu Ke-Yang , Wang Shuai , Lu Tong , Lu Ying-Fa , Deng Wei-Chao , Li Hou-Min
Published: June 2022

Conventional neural networks tend to fall into local extremum on large datasets, while the research on the strength of rubber concrete using intelligent algorithms to optimize artificial neural networks is limited. Therefore, to improve the prediction accuracy of rubber concrete strength, an artificial neural network model with hybrid algorithm optimization was developed in this study. The main strategy is to mix the simulated annealing (SA) algorithm with the particle swarm optimization (PSO) algorithm, using the SA algorithm to compensate for the weak global search capability of the PSO algorithm at a later stage while changing the inertia factor of the PSO algorithm to an adaptive state. For this purpose, data were first collected from the published literature to create a database. Next, ANN and PSO-ANN models are also built for comparison while four evaluation metrics, MSE, RMSE, MAE, and R2, were used to assess the model performance. Finally, compared with empirical formulations and other neural network models, the result shows that the proposed optimized artificial neural network model successfully improves the accuracy of predicting the strength of rubber concrete. This provides a new option for predicting the strength of rubber concrete.
Use of Xenogenic Collagen Matrices in Peri-Implant Soft Tissue Volume Augmentation: A Critical Review on the Current Evidence and New Technique Presentation
Authors: De Annuntiis Carlo , Testarelli Luca , Guarnieri Renzo
Published: June 2022

Plastic peri-implant surgical procedures aiming to increase soft tissue volume around dental implants have long been well-described. These are represented by: pedicle soft tissue grafts (rotational flap procedures and advanced flap procedures) and free soft tissue grafts (epithelialized, also called free gingival graft (FGG), and non-epithelialized, also called, connective tissue graft (CTG) or a combination of both. To bypass the drawback connected with autologous grafts harvesting, xenogenic collagen matrices (XCM)s and collagen-based matrices derived from porcine dermis (PDXCM)s have been introduced, as an alternative, in plastic peri-implant procedures. Aim: This review is aimed to evaluate and to critically analyze the available evidence on the effectiveness of XCMs and PDXCMs in soft tissue volume augmentation around dental implants. Moreover, a clinical case with a new soft tissue grafting procedure technique (Guided Soft Tissue Regeneration, GSTR) is presented. Material and Methods: An electronic search was performed on the MEDLINE database, SCOPUS, Cochrane Library and Web of Science. The electronic search provided a total of 133 articles. One hundred and twenty-eight not meeting the inclusion criteria were excluded. Seven articles of human randomized clinical trials were selected. A total number of 108 patients were treated with CTG, and 110 patients with XCM. Results: in peri-implant soft tissue augmentation procedures, XCMs seem an effective alternative to CTGs, associated with lower patient morbidity and lower operative times.
Crack Texture Feature Identification of Fiber Reinforced Concrete Based on Deep Learning
Authors: Zhou Shuangxi , Pan Yuan , Huang Xiaosheng , Yang Dan , Ding Yang , Duan Runtao
Published: June 2022

Structural cracks in concrete have a significant influence on structural safety, so it is necessary to detect and monitor concrete cracks. Deep learning is a powerful tool for detecting cracks in concrete structures. However, it requires a large quantity of training samples and is costly in terms of computational time. In order to solve these difficulties, a deep learning target detection framework combining texture features with concrete crack data is proposed. Texture features and pre-processed concrete data are merged to increase the number of feature channels in order to reduce the demand of training samples for the model and improve training speed. With this framework, concrete crack detection can be realized even with a limited number of samples. To accomplish this aim, self-made steel fiber reinforced concrete crack data is used for comparison between our framework and those without texture feature mergence or pre-processed concrete data. The experimental results show that the number of parameters that need to be fitted in the model training and training time can be correspondingly reduced and the detection accuracy can also be improved.
Experimental-Numerical Study on the Flexural Ultimate Capacity of Prestressed Concrete Box Girders Subjected to Collision
Authors: Li Yong , Yu Zijie , Wu Qifan , Liu Yongqian , Wang Shang
Published: June 2022

Precise evaluation for flexural ultimate capacity of bridges which are subjected to the collision of over-height trucks is essential for making decisions on corresponding maintenance, strengthening or replacement. When the span of a cross-line continuous bridge with a double-box girder was hit by an overly high vehicle, the concrete floor of one girder was severely damaged, and part of the prestressed strands and reinforcements in the girder were broken. After the double-box girder was removed and separated into two single box girders, the ultimate flexural capacity of both box girders was studied by destructive tests, and a comparison was made between the damaged and undamaged girders. Moreover, finite element analysis was conducted to simulate the failure process. The results show that the flexural bearing capacity of the damaged box girder decreased by 33%, but it was still 1.07 times greater than the design bearing capacity, which basically meets the design requirements. Also, the damaged box girder showed a desirable serviceable limit state for three-axle vehicles and five-axle vehicles, but showed an undesirable serviceable limit state for six-axle vehicles. This study shows that repairing or strengthening the damaged span may be better than demolishing and rebuilding the whole superstructure bridge.
Effect of Sulfate Ions on Galvanized Post-Tensioned Steel Corrosion in Alkaline Solutions and the Interaction with Other Ions
Authors: Bonilla Andrés , Argiz Cristina , Moragues Amparo , Gálvez Jaime C.
Published: June 2022

Zinc protection of galvanized steel is initially dissolved in alkaline solutions. However, a passive layer is formed over time which protects the steel from corrosion. The behavior of galvanized steel exposed to strong alkaline solutions (pH values of 12.7) with a fixed concentration of sulfate ions of 0.04 M is studied here. Electrochemical measurement techniques such as corrosion potential, linear polarization resistance and electrochemical impedance spectroscopy are used. Synergistic effects of sulfate ions are also studied together with other anions such as chloride Cl− or bicarbonate ion HCO3− and with other cations such as calcium Ca2+, ammonium NH4+ and magnesium Mg2+. The presence of sulfate ions can also depassivate the steel, leading to a corrosion current density of 0.3 µA/cm2 at the end of the test. The presence of other ions in the solution increases this effect. The increase in corrosion current density caused by cations and anions corresponds to the following orders (greater to lesser influence): NH4+ > Ca2+ > Mg2+ and HCO3− > Cl− > SO42−.
Morphological Component Analysis-Based Hidden Markov Model for Few-Shot Reliability Assessment of Bearing
Authors: Feng Yi , Li Weijun , Zhang Kai , Li Xianling , Cai Wenfang , Liu Ruonan
Published: June 2022

Reliability is of great significance in ensuring the safe operation of modern industry, which mainly relies on data analysis and life tests. However, as the life of mechanical systems becomes increasingly longer with the rapid development of the manufacturing industry, the collection of historical failure data becomes progressively more time-consuming. In this paper, a few-shot reliability assessment approach is proposed in order to overcome the dependence on historical data. Firstly, the vibration response of a bearing was illustrated. Then, based on a vibration response analysis, a morphological component analysis (MCA) method based on sparse representation theory was used to decompose vibration signals and extract impulse signals. After the impulse components’ reconstruction, their statistical indexes were utilized as the input observation vector of a Mixture of Gaussians Hidden Markov Model (MoG-HMM) for a reliability estimation. Finally, the experimental dataset of an aerospace bearing was analyzed via the proposed method. The comparison results illustrate the effectiveness of the proposed method of a few-shot reliability assessment.
A Bounded Archiver for Hausdorff Approximations of the Pareto Front for Multi-Objective Evolutionary Algorithms
Authors: Hernández Castellanos Carlos Ignacio , Schütze Oliver
Published: June 2022

Multi-objective evolutionary algorithms (MOEAs) have been successfully applied for the numerical treatment of multi-objective optimization problems (MOP) during the last three decades. One important task within MOEAs is the archiving (or selection) of the computed candidate solutions, since one can expect that an MOP has infinitely many solutions. We present and analyze in this work ArchiveUpdateHD, which is a bounded archiver that aims for Hausdorff approximations of the Pareto front. We show that the sequence of archives generated by ArchiveUpdateHD yields under certain (mild) assumptions with a probability of one after finitely many steps a Δ+-approximation of the Pareto front, where the value Δ+ is computed by the archiver within the run of the algorithm without any prior knowledge of the Pareto front. The knowledge of this value is of great importance for the decision maker, since it is a measure for the “completeness” of the Pareto front approximation. Numerical results on several well-known academic test problems as well as the usage of ArchiveUpdateHD as an external archiver within three state-of-the-art MOEAs indicate the benefit of the novel strategy.
Interdisciplinary Approach of Establishing PDAC Resectability: Biochemical, Radiological and NAT Regimen Prognostic Factors—Literature Review
Authors: Gulla Aiste , Hashimoto Daisuke , Wagner Doris , Damaseviciute Ryte , Strupas Kestutis , Satoi Sohei
Published: June 2022

Background and Objectives: Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal tumors, with a 5-year overall survival rate of less than 10%. To date, curative surgical resection remains the only favorable option for improving patients’ survival. However, there is no consensus on which prognostic biochemical, radiological markers or neoadjuvant therapy regimens would benefit patients the most. Materials and Methods: A literature review was performed focusing on overall survival, R0 resection, 30-day mortality, adverse events (AEs), and elevated biomarkers. The electronic databases were searched from 2015 to 2020. Results: We reviewed 22 independent studies. In total, 20 studies were retrospective single- or multi-center reviews, while 2 studies were prospective Phase II trials. Conclusions: Patients with borderline resectable or locally advanced PDAC, who received neoadjuvant therapy (NAT) and surgery, have significantly better survival rates. The CA 19-9 biomarker levels in the neoadjuvant setting should be evaluated and considered as a specific biomarker for tumor resectability and overall survival.
Quantitative Proteomics Reveals That ADAM15 Can Have Proteolytic-Independent Functions in the Steady State
Authors: Yang Chun-Yao , Bonelli Simone , Calligaris Matteo , Carreca Anna Paola , Müller Stephan A. , Lichtenthaler Stefan F. , Troeberg Linda , Scilabra Simone D.
Published: June 2022

A disintegrin and metalloproteinase 15 (ADAM15) is a member of the ADAM family of sheddases. Its genetic ablation in mice suggests that ADAM15 plays an important role in a wide variety of biological functions, including cartilage homeostasis. Nevertheless, while the substrate repertoire of other members of the ADAM family, including ADAM10 and ADAM17, is largely established, little is known about the substrates of ADAM15 and how it exerts its biological functions. Herein, we used unbiased proteomics to identify ADAM15 substrates and proteins regulated by the proteinase in chondrocyte-like HTB94 cells. ADAM15 silencing did not induce major changes in the secretome composition of HTB94 cells, as revealed by two different proteomic approaches. Conversely, overexpression of ADAM15 remodeled the secretome, with levels of several secreted proteins being altered compared to GFP-overexpressing controls. However, the analysis did not identify potential substrates of the sheddase, i.e., transmembrane proteins released by ADAM15 in the extracellular milieu. Intriguingly, secretome analysis and immunoblotting demonstrated that ADAM15 overexpression increased secreted levels of tissue inhibitor of metalloproteinases 3 (TIMP-3), a major regulator of extracellular matrix turnover. An inactive form of ADAM15 led to a similar increase in the inhibitor, indicating that ADAM15 regulates TIMP-3 secretion by an unknown mechanism independent of its catalytic activity. In conclusion, high-resolution quantitative proteomics of HTB94 cells manipulated to have increased or decreased ADAM15 expression did not identify canonical substrates of the proteinase in the steady state, but it revealed that ADAM15 can modulate the secretome in a catalytically-independent manner.
Mechanical Properties of L-Shaped Column Composed of RAC-Filled Steel Tubes under Eccentric Compression
Authors: Ma Tengfei , Chen Zhihua , Du Yansheng , Zhou Ting , Zhang Yutong
Published: June 2022

In this paper, the eccentric compression test of seven specimens was conducted to explore the application possibility of recycled aggregate concrete (RAC) in L-shaped columns composed of concrete-filled steel tubes (CFST). The main parameter is the replacement ratio of recycled coarse aggregates (RCA), and an L-shaped column of composed hollow steel tubes was set as a control. The test results indicated that the bearing capacity and stiffness of the L-shaped column composed of RAC-filled steel tubes (RACFSTs) are better than those of the L-shaped column composed of hollow steel tubes. The compressive strength of concrete is reduced by 73.1% as the replacement ratio of RCA increases from 0 to 100%, while that of the column is merely reduced by 23.9%. The strength disadvantage of RAC is compensated by the confinement of steel tubes. Besides, the result of the eccentric compression test (80 mm eccentricity) was compared with the axial compression test (0 mm eccentricity). The increase in eccentricity reduced the bearing capacity and ductility due to additional bending moments. The finite element model (FEM) was established by software ANSYS to compare with the experimental results. The bearing capacity deviation of FEM is 4.23~6.56%. The parametric analysis was carried out to summarize the influence of parameters such as eccentricity, material strength, and steel tube thickness. With the increase of eccentricity, the bearing capacity of the RACFST decreases gradually. In engineering design, the bearing capacity of the RACFST can be improved by increasing the strength and thickness of the steel.
Non-Invasive O-Toluidine Monitoring during Regional Anaesthesia with Prilocaine and Detection of Accidental Intravenous Injection in an Animal Model
Authors: Brock Beate , Fuchs Patricia , Kamysek Svend , Walther Udo , Traxler Selina , Pugliese Giovanni , Miekisch Wolfram , Schubert Jochen K. , Trefz Phillip
Published: June 2022

Regional anaesthesia is well established as a standard method in clinical practice. Currently, the local anaesthetics of amino-amide types such as prilocaine are frequently used. Despite routine use, complications due to overdose or accidental intravenous injection can arise. A non-invasive method that can indicate such complications early would be desirable. Breath gas analysis offers great potential for the non-invasive monitoring of drugs and their volatile metabolites. The physicochemical properties of o-toluidine, the main metabolite of prilocaine, allow its detection in breath gas. Within this study, we investigated whether o-toluidine can be monitored in exhaled breath during regional anaesthesia in an animal model, if correlations between o-toluidine and prilocaine blood levels exist and if accidental intravenous injections are detectable by o-toluidine breath monitoring. Continuous o-toluidine monitoring was possible during regional anaesthesia of the cervical plexus and during simulated accidental intravenous injection of prilocaine. The time course of exhaled o-toluidine concentrations considerably differed depending on the injection site. Intravenous injection led to an immediate increase in exhaled o-toluidine concentrations within 2 min, earlier peak and higher maximum concentrations, followed by a faster decay compared to regional anaesthesia. The strength of correlation of blood and breath parameters depended on the injection site. In conclusion, real time monitoring of o-toluidine in breath gas is possible by means of PTR-ToF-MS. Since simulated accidental intravenous injection led to an immediate increase in exhaled o-toluidine concentrations within 2 min and higher maximum concentrations, monitoring exhaled o-toluidine may potentially be applied for the non-invasive real-time detection of accidental intravenous injection of prilocaine.
Circulating PCSK9 Linked to Dyslipidemia in Lebanese Schoolchildren
Authors: Azar Yara , Gannagé-Yared Marie-Hélène , Naous Elie , Ayoub Carine , Abou Khalil Yara , Chahine Elise , Elbitar Sandy , Ghaleb Youmna , Boileau Catherine , Varret Mathilde , El Khoury Petra , Abifadel Marianne
Published: June 2022

In adults, elevated levels of circulating Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) have been associated with increased Low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and worse cardiovascular outcomes. However, few studies analyzed the relation between PCSK9 and lipid parameters in pediatric populations. The aim of our study is to evaluate the distribution and the correlation of serum PCSK9 levels with lipid parameters in a sample of Lebanese school children. Using an immunofluorescence assay, we measured serum PCSK9 levels in 681 school children recruited from ten public and private Lebanese schools. We analyzed the association between PCSK9 and age, sex, Body Mass Index (BMI), and lipid parameters (total cholesterol (TC), LDL-C, TG, High-density lipoprotein cholesterol (HDL-C), non-HDL-C, and lipoprotein (a) (Lp(a)). Serum PCSK9 levels were significantly correlated with TC, LDL-C, and non-HDL-C (p value < 0.0001) but not with TG, HDL-C, and Lp(a). PCSK9 levels were also significantly higher in children with high TC, LDL-C, and non-HDL-C (p values = 0.0012, 0.0002, 0.001, respectively). No significant gender differences in PCSK9 were found. In addition, no significant associations between PCSK9 and both age and BMI percentiles were observed. In girls, no difference in PCSK9 values was observed according to menarche while in boys, testosterone levels were not significantly associated with PCSK9. Serum PCSK9 levels were significantly correlated with TC, LDL-C, and non-HDL-C levels. Further studies are needed to find if PCSK9 measurements have an additional value to predict future cardiovascular outcomes in pediatric populations.
A Novel Modification of Copper (II) Phthalocyanine Particles towards Electrophoretic Displays
Authors: Wang Yao , Zhang Zhi , Chen Qun , Ye Caihong , Zhang Jiahao , Gao Qingguo , Liu Liming , Yang Jianjun , Pan Xinjian , Miao Yu , Chi Feng , Jin Mingliang
Published: June 2022

Electrophoretic display (EPD) is a popular display technology in recent years. The core of the EPD is electrophoretic particles, and its Zeta potential has an important impact on EPDs. In this work, a method using pyrrolidine mono ionic liquid was proposed to improve the Zeta potential of electrophoretic particles: Copper (II) phthalocyanine pigment was modified with mono ionic liquid 1-Butyl-1-methylpyrrolidinium bromide. The characterization results show that the mono ionic liquid had been successfully coated on pigment particles. At the same time, the dispersion and stability of particles were improved. The modified Copper (II) phthalocyanine pigment could be stably dispersed in tetrachloroethylene for more than 20 days. The Zeta potential increased from 32.42 mV to 49.91 mV, increasing by 53.95%. Finally, the prepared blue electrophoretic particles were compounded with white titanium dioxide to prepare blue and white dual-color electrophoretic dispersion, and then an EPD cell was designed to test its performance. The results show that the prepared electrophoretic dispersion can realize reversible reciprocating motion. Therefore, because of the unique structure and properties of pyrrolidine mono ionic liquids, the blue nanoparticles prepared with pyrrolidine ionic liquids as charge control agents in this study can be used as excellent candidate materials for EPD.
Characterization of Fluidic-Barrier-Based Particle Generation in Centrifugal Microfluidics
Authors: Madadelahi Masoud , Azimi-Boulali Javid , Madou Marc , Martinez-Chapa Sergio
Published: June 2022

The fluidic barrier in centrifugal microfluidic platforms is a newly introduced concept for making multiple emulsions and microparticles. In this study, we focused on particle generation application to better characterize this method. Because the phenomenon is too fast to be captured experimentally, we employ theoretical models to show how liquid polymeric droplets pass a fluidic barrier before crosslinking. We explain how secondary flows evolve and mix the fluids within the droplets. From an experimental point of view, the effect of different parameters, such as the barrier length, source channel width, and rotational speed, on the particles’ size and aspect ratio are investigated. It is demonstrated that the barrier length does not affect the particle’s ultimate velocity. Unlike conventional air gaps, the barrier length does not significantly affect the aspect ratio of the produced microparticles. Eventually, we broaden this concept to two source fluids and study the importance of source channel geometry, barrier length, and rotational speed in generating two-fluid droplets.
Developing Meso and Microholes by Spark-Erosion Based Drilling Processes: A Critical Review
Authors: Chaubey Sujeet Kumar , Gupta Kapil
Published: June 2022

The increased demand for miniature components has drawn the attention of researchers, engineers, and industry users to manufacture precision micro and mesoholes on foils, sheets, and plates made from a variety of engineering materials. These days, micro-drilling is extensively being adopted as a fundamental operation in all kinds of smart manufacturing industries to make different types of microholes, such as through holes, blind holes, and taper holes on micro-parts and components. Drilled holes with a diameter of less than 1 mm are referred to as microholes, while drilled holes whose diameter ranges between 1 and 10 mm are known as mesoholes. Meso and microholes are commonly referred to as fine-holes. Modern or advanced drilling processes are mostly used to drill microholes from a variety of materials. This paper presents an extensive review of the previous research conducted on the drilling of fine holes (meso and micro size) by spark- erosion-based processes along with highlighting work and tool electrode materials, specifications of drilled holes, types of microholes (through or blind holes), process parameters, performance measures, and key findings. The paper aims to facilitate researchers and scholars by highlighting the capabilities of spark erosion machining, drilling, and its variants to fabricate miniature holes. The paper ends with a conclusion and future research directions to encourage further work in this area.
A Hybrid Algorithm for Noise Suppression of MEMS Accelerometer Based on the Improved VMD and TFPF
Authors: Zhou Yongjun , Cao Huiliang , Guo Tao
Published: June 2022

High-G MEMS accelerometer (HGMA) is a new type of sensor; it has been widely used in high precision measurement and control fields. Inevitably, the accelerometer output signal contains random noise caused by the accelerometer itself, the hardware circuit and other aspects. In order to denoise the HGMA’s output signal to improve the measurement accuracy, the improved VMD and TFPF hybrid denoising algorithm is proposed, which combines variational modal decomposition (VMD) and time-frequency peak filtering (TFPF). Firstly, VMD was optimized by the multi-objective particle swarm optimization (MOPSO), then the best decomposition parameters [kbest, abest] could be obtained, in which the permutation entropy (PE) and fuzzy entropy (FE) were selected for MOPSO as fitness functions. Secondly, the accelerometer voltage output signals were decomposed by the improved VMD, then some intrinsic mode functions (IMFs) were achieved. Thirdly, sample entropy (SE) was introduced to classify those IMFs into information-dominated IMFs or noise-dominated IMFs. Then, the short-window TFPF was selected for denoising information-dominated IMFs, while the long-window TFPF was selected for denoising noise-dominated IMFs, which can make denoising more targeted. After reconstruction, we obtained the accelerometer denoising signal. The denoising results of different denoising algorithms in the time and frequency domains were compared, and SNR and RMSE were taken as denoising indicators. The improved VMD and TFPF denoising method has a smaller signal distortion and stronger denoising ability, so it can be adopted to denoise the output signal of the High-G MEMS accelerometer to improve its accuracy.
Electrodeposition of CoxNiVyOz Ternary Nanopetals on Bare and rGO-Coated Nickel Foam for High-Performance Supercapacitor Application
Authors: Seyed-Talebi Seyedeh , Cheraghizade Mohsen , Beheshtian Javad , Kuan Chun-Hsiao , Diau Eric
Published: June 2022

We report a simple strategy to grow a novel cobalt nickel vanadium oxide (CoxNiVyOz) nanocomposite on bare and reduced-graphene-oxide (rGO)-coated nickel foam (Ni foam) substrates. In this way, the synthesized graphene oxide is coated on Ni foam, and reduced electrochemically with a negative voltage to prepare a more conductive rGO-coated Ni foam substrate. The fabricated electrodes were characterized with a field-emission scanning electron microscope (FESEM), energy-dispersive X-ray spectra (EDX), X-ray photoelectron spectra (XPS), and Fourier-transform infrared (FTIR) spectra. The electrochemical performance of these CoxNiVyOz-based electrode materials deposited on rGO-coated Ni foam substrate exhibited superior specific capacitance 701.08 F/g, which is more than twice that of a sample coated on bare Ni foam (300.31 F/g) under the same experimental conditions at current density 2 A/g. Our work highlights the effect of covering the Ni foam surface with a rGO film to expedite the specific capacity of the supercapacitors. Despite the slightly decreased stability of a CoxNiVyOz-based electrode coated on a Ni foam@rGO substrate, the facile synthesis, large specific capacitance, and preservation of 92% of the initial capacitance, even after running 5500 cyclic voltammetric (CV) scans, indicate that the CoxNiVyOz-based electrode is a promising candidate for high-performance energy-storage devices.
Perioperative Administration of Cystine and Theanine Suppresses Inflammation and Facilitates Early Rehabilitation and Recovery after Esophagectomy: A Randomized, Double-Blind, Controlled Clinical Trial
Authors: Okamoto Hiroshi , Taniyama Yusuke , Sakurai Tadashi , Kodama Gaku , Sato Chiaki , Fukutomi Toshiaki , Ozawa Yohei , Ishida Hirotaka , Koseki Ken , Yamauchi Takuro , Nakano Toru , Unno Michiaki , Kamei Takashi
Published: June 2022

Oral administration of cystine and theanine (CT) increases glutathione levels to modulate the inflammatory response, which has yet to be sufficiently explored for patients’ recovery and early rehabilitation. We planned a randomized, double-blind, placebo-controlled trial to determine whether perioperative oral administration of CT promotes recovery after esophagectomy. Patients were randomized into either CT or placebo groups, who received preoperative and postoperative treatments for 4 and 13 days, respectively. The main outcome measures were triaxial accelerometer readings, inflammation indicators, a 6 min walk test (6MWT), and a quality of life questionnaire (QoR-40). The study involved 32 patients. Although the CT group (n = 16) showed better patient activity across the investigated period, there was no significant difference between the two groups. However, white blood cell count on postoperative days (POD) 2 and 10, neutrophil count (POD 2, 7, and 10), and C-reactive protein level (POD 13) in the CT group were significantly lower than in the placebo group. Furthermore, 6MWT on POD 7 and QoR-40 on POD 13 were significantly higher in the CT group than those in the placebo group. This study suggests that perioperative administration of CT may contribute to early recovery and rehabilitation after esophagectomy via suppression of inflammatory response.