bluuebunny/crossref_metadata_2025 huggingface dataset:
Cross-notational semantic priming between symbolic and nonsymbolic numerosity
Authors: Herrera Amparo , Macizo Pedro
Published: May 2008

Symbolic and nonsymbolic numerosities produce similar behavioural effects and activate the same brain areas. These results have usually been interpreted in terms of a common, notation-independent magnitude representation. However, semantic priming between symbolic and nonsymbolic inputs has been somehow elusive (e.g., Koechlin, Naccache, Block, & Dehaene, 1999). In Experiment 1, we looked at whether cross-notational semantic priming depends on exact numerical meaning. Dice faces and digits were mixed as prime and target. Semantic priming occurred when prime and target were in the same notation as much as when they were in different notation. In Experiment 2, we found cross-notation semantic priming even when the nonsymbolic numerosity was presented as a set of random dots. Priming, however, occurred only from sets of dots to digit, not vice versa. These data support the computational model recently proposed by Verguts and Fias (2004; Verguts, Fias, & Stevens, 2005).
Goal-driven modulation as a function of time in saccadic target selection
Authors: Van Zoest Wieske , Donk Mieke
Published: May 2008

Four experiments were performed to investigate the contribution of goal-driven modulation in saccadic target selection as a function of time. Observers were required to make an eye movement to a prespecified target that was concurrently presented with multiple nontargets and possibly one distractor. Target and distractor were defined in different dimensions (orientation dimension and colour dimension in Experiment 1), or were both defined in the same dimension (i.e., both defined in the orientation dimension in Experiment 2, or both defined in the colour dimension in Experiments 3 and 4). The identities of target and distractor were switched over conditions. Speed–accuracy functions were computed to examine the full time course of selection in each condition. There were three major results. First, the ability to exert goal-driven control increased as a function of response latency. Second, this ability depended on the specific target–distractor combination, yet was not a function of whether target and distractor were defined within or across dimensions. Third, goal-driven control was available earlier when target and distractor were dissimilar than when they were similar. It was concluded that the influence of goal-driven control in visual selection is not all or none, but is of a continuous nature.
Processing Spatial Layout by Perception and Sensorimotor Interaction
Authors: Bridgeman Bruce , Hoover Merrit
Published: May 2008

Everyone has the feeling that perception is usually accurate—we apprehend the layout of the world without significant error, and therefore we can interact with it effectively. Several lines of experimentation, however, show that perceived layout is seldom accurate enough to account for the success of visually guided behaviour. A visual world that has more texture on one side, for example, induces a shift of the body's straight ahead to that side and a mislocalization of a small target to the opposite side. Motor interaction with the target remains accurate, however, as measured by a jab with the finger. Slopes of hills are overestimated, even while matching the slopes of the same hills with the forearm is more accurate. The discrepancy shrinks as the estimated range is reduced, until the two estimates are hardly discrepant for a segment of a slope within arm's reach. From an evolutionary standpoint, the function of perception is not to provide an accurate physical layout of the world, but to inform the planning of future behaviour. Illusions—inaccuracies in perception—are perceived as such only when they can be verified by objective means, such as measuring the slope of a hill, the range of a landmark, or the location of a target. Normally such illusions are not checked and are accepted as reality without contradiction.
Grasp Cueing shows Obligatory Attention to Action Goals
Authors: Fischer Martin H. , Prinz Julia , Lotz Katharina
Published: May 2008

To understand the grounding of cognitive mechanisms in perception and action, we used a simple detection task to determine how long it takes to predict an action goal from the perception of grasp postures and whether this prediction is under strategic control. Healthy observers detected visual probes over small or large objects after seeing either a precision grip or a power grip posture. Although the posture was uninformative it induced attention shifts to the grasp-congruent object within 350 ms. When the posture predicted target appearance over the grasp-incongruent object, observers’ initial strategic allocation of attention was overruled by the congruency between grasp and object. These results might help to characterize the human mirror neuron system and reveal how joint attention tunes early perceptual processes toward action prediction.
Motor Resonance and Linguistic Focus
Authors: Taylor Lawrence J. , Zwaan Rolf A.
Published: May 2008

Previous studies have demonstrated that verbal descriptions of actions activate compatible motor responses (Glenberg & Kaschak, 2002; Zwaan & Taylor, 2006). The present study replicates previous findings showing that, within a sentence, such activation is localized on the verb that denotes the action. Moreover, motor resonance is found to yield to linguistic focus. If a postverbal adverb maintains focus on a matching action (“slowly” or “quickly”), motor resonance occurs, but if the adverb shifts the focus to the agent (e.g., “obediently” or “eagerly”), a cessation of motor resonance ensues. These findings are discussed within the context of theories of motor resonance, action understanding, mental simulation, and linguistic focus.
Processing Abstract Language Modulates Motor System Activity
Authors: Glenberg Arthur M. , Sato Marc , Cattaneo Luigi , Riggio Lucia , Palumbo Daniele , Buccino Giovanni
Published: May 2008

Embodiment theory proposes that neural systems for perception and action are also engaged during language comprehension. Previous neuroimaging and neurophysiological studies have only been able to demonstrate modulation of action systems during comprehension of concrete language. We provide neurophysiological evidence for modulation of motor system activity during the comprehension of both concrete and abstract language. In Experiment 1, when the described direction of object transfer or information transfer (e.g., away from the reader to another) matched the literal direction of a hand movement used to make a response, speed of responding was faster than when the two directions mismatched (an action–sentence compatibility effect). In Experiment 2, we used single-pulse transcranial magnetic stimulation to study changes in the corticospinal motor pathways to hand muscles while reading the same sentences. Relative to sentences that do not describe transfer, there is greater modulation of activity in the hand muscles when reading sentences describing transfer of both concrete objects and abstract information. These findings are discussed in relation to the human mirror neuron system.
Effects of stimulus features and instruction on response coding, selection, and inhibition: Evidence from repetition effects under task switching
Authors: Druey Michel D. , Hübner Ronald
Published: May 2008

The coding of stimuli and responses is crucial for human behaviour. Here, we focused primarily on the response codes (or response categories). As a method, we applied a combined dual-task and task-switch paradigm with a fixed task-to-hand mapping. Usually, negative effects (i.e., costs) are observed for response category repetitions under task switching. However, in several previous studies it has been proposed that such repetition effects do not occur, if the stimulus categories (e.g., “odd” if digits have to be classified according to their parity feature) are unequivocally mapped to specific responses. Our aim was to test this hypothesis. In the present experiments, we were able to distinguish between three different types of possible response codes. The results show that the participants generally code their responses according to abstract response features (left/right, or index/middle finger). Moreover, the spatial codes were preferred over the finger-type codes even if the instructions stressed the latter. This preference, though, seemed to result from a stimulus–response feature overlap, so that the spatial response categories were primed by the respective stimulus features. If there was no such overlap, the instructions determined which type of response code was involved in response selection and inhibition.
Are phonological influences on lexical (mis)selection the result of a monitoring bias?
Authors: Severens Els , Ratinckx Elie , Ferreira Victor S. , Hartsuiker Robert J.
Published: May 2008

A monitoring bias account is often used to explain speech error patterns that seem to be the result of an interactive language production system, like phonological influences on lexical selection errors. A biased monitor is suggested to detect and covertly correct certain errors more often than others. For instance, this account predicts that errors that are phonologically similar to intended words are harder to detect than those that are phonologically dissimilar. To test this, we tried to elicit phonological errors under the same conditions as those that show other kinds of lexical selection errors. In five experiments, we presented participants with high cloze probability sentence fragments followed by a picture that was semantically related, a homophone of a semantically related word, or phonologically related to the (implicit) last word of the sentence. All experiments elicited semantic completions or homophones of semantic completions, but none elicited phonological completions. This finding is hard to reconcile with a monitoring bias account and is better explained with an interactive production system. Additionally, this finding constrains the amount of bottom-up information flow in interactive models.
Implicit sequence learning in a search task
Authors: Jiménez Luis , Vázquez Gustavo A.
Published: May 2008

This study investigated the effects of selection demands on implicit sequence learning. Participants in a search condition looked for a target among seven distractors and responded on the target identity. The responses followed a deterministic sequence, and sequence learning was compared to that found in two control conditions in which the targets were presented alone, either at a central location or over a series of unpredictable locations. Sequence learning was obtained in all conditions, and it was equivalent for the two variable location conditions, regardless of the perceptual demands. Larger effects of learning were observed in the central location, both on the indirect measures and on the measures taken from a cued-generation task. The expression of learning decreased selectively in this condition when the sequence validity was reduced over a test block. These results are consistent with the claims that implicit and explicit learning are mixed in this central condition and that implicit learning is not affected by selection difficulty.
A decrease in conjunction error rates across lags on a continuous recognition task: A robust pattern
Authors: Jones Todd C. , Atchley Paul
Published: May 2008

In four experiments, the lag retention interval from parent words (e.g., blackmail, jailbird) to a conjunction word ( blackbird) was manipulated in a continuous recognition task. Alterations to the basic procedure of Jones and Atchley (2002) were employed in Experiments 1 and 2 to bolster recollection to reject conjunction lures, yet conjunction error rates still decreased across lags of 1 to 20 words. Experiment 3 and a multiexperiment analysis examined the increments of forgetting in familiarity across lags of 1–20 words. Finally, in Experiment 4, participants attempted to identify conjunction probes as “old”, and the data were contrasted with those from a previous experiment (Jones & Atchley, 2002, Exp. 1), in which participants attempted not to identify conjunction probes as “old”. In support of earlier findings, the decrease in familiarity across lags of 1–20 words appears robust, with a constant level of weak recollection occurring for parent words.
Kinetic Potential Influences Visual and Remote Haptic Perception of Affordances for Standing on an Inclined Surface
Authors: Malek Eric A. , Wagman Jeffrey B.
Published: May 2008

The ability of a perceiver–actor to perform a particular behaviour depends on their ability to generate and control the muscular forces required to perform that behaviour. If an intended behaviour is to be successful, perception must be relative to this ability. We investigated whether perceiver–actors were sensitive to how changes in their mass distribution influenced their ability to stand on an inclined surface. Participants reported whether they would be able to stand on an inclined surface while wearing a weighted backpack on their back, while wearing a weighted backpack on their front, and while not wearing a weighted backpack. In addition, participants performed this task by either viewing the surface or exploring it with a hand-held rod (while blindfolded). The results showed that perception of affordances for standing on the inclined surface depended on how the backpack influenced the ability of the participant to stand on the surface. Specifically, perceptual boundaries occurred at steeper inclinations when participants wore the backpack on their front than when they wore it on their back. Moreover, this pattern occurred regardless of the perceptual modality by which the participants explored the inclined surface.
Time, Number and Length: Similarities and Differences in Discrimination in Adults and Children
Authors: Droit-Volet Sylvie , Clément Angélique , Fayol Michel
Published: May 2008

The aim of this study was to focus on similarities in the discrimination of three different quantities—time, number, and line length—using a bisection task involving children aged 5 and 8 years and adults, when number and length were presented nonsequentially (Experiment 1) and sequentially (Experiment 2). In the nonsequential condition, for all age groups, although to a greater extent in the younger children, the psychophysical functions were flatter, and the Weber ratio higher for time than for number and length. Number and length yielded similar psychophysical functions. Thus, sensitivity to time was lower than that to the other quantities, whether continuous or not. However, when number and length were presented sequentially (Experiment 2), the differences in discrimination performance between time, number, and length disappeared. Furthermore, the Weber ratio values as well as the bisection points for all quantities presented sequentially appeared to be close to that found for duration in the nonsequential condition. The results are discussed within the framework of recent theories suggesting a common mechanism for all analogical quantities.
Affective significance enhances covert attention: Roles of anxiety and word familiarity
Authors: Calvo Manuel G. , Eysenck Michael W.
Published: May 2008

To investigate the processing of emotional words by covert attention, threat-related, positive, and neutral word primes were presented parafoveally (2.2° away from fixation) for 150 ms, under gaze-contingent foveal masking, to prevent eye fixations. The primes were followed by a probe word in a lexical-decision task. In Experiment 1, results showed a parafoveal threat–anxiety superiority: Parafoveal prime threat words facilitated responses to probe threat words for high-anxiety individuals, in comparison with neutral and positive words, and relative to low-anxiety individuals. This reveals an advantage in threat processing by covert attention, without differences in overt attention. However, anxiety was also associated with greater familiarity with threat words, and the parafoveal priming effects were significantly reduced when familiarity was covaried out. To further examine the role of word knowledge, in Experiment 2, vocabulary and word familiarity were equated for low- and high-anxiety groups. In these conditions, the parafoveal threat–anxiety advantage disappeared. This suggests that the enhanced covert-attention effect depends on familiarity with words.
The Role of Meaning in Contextual Cueing: Evidence from Chess Expertise
Authors: Brockmole James R. , Hambrick David Z. , Windisch David J. , Henderson John M.
Published: May 2008

In contextual cueing, the position of a search target is learned over repeated exposures to a visual display. The strength of this effect varies across stimulus types. For example, real-world scene contexts give rise to larger search benefits than contexts composed of letters or shapes. We investigated whether such differences in learning can be at least partially explained by the degree of semantic meaning associated with a context independently of the nature of the visual information available (which also varies across stimulus types). Chess boards served as the learning context as their meaningfulness depends on the observer's knowledge of the game. In Experiment 1, boards depicted actual game play, and search benefits for repeated boards were 4 times greater for experts than for novices. In Experiment 2, search benefits among experts were halved when less meaningful randomly generated boards were used. Thus, stimulus meaningfulness independently contributes to learning context–target associations.
The detection of temporally defined objects does not require focused attention
Authors: Pinto Yaïr , Olivers Christian N. L. , Theeuwes Jan
Published: May 2008

Perceptual grouping is crucial to distinguish objects from their background. Recent studies have shown that observers can detect an object that does not have any unique qualities other than unique temporal properties. A crucial question is whether focused attention is needed for this type of grouping. In two visual search experiments, we show that searching for an object defined by temporal grouping can occur in parallel. These findings suggest that focused attention is not needed for temporal grouping to occur. It is proposed that temporal grouping may occur because the neurons representing the changing object elements adopt firing frequencies that cause the visual system to bind these elements together without the need for focused attention.
Who is dominating the Dutch neighbourhood? On the role of subsyllabic units in Dutch nonword reading
Authors: Loncke Maaike , Martensen Heike , van Heuven Walter J. B. , Sandra Dominiek
Published: May 2008

To assess the role of the subsyllabic units onset–nucleus (ON; spa rk) and rime ( sp ark) in Dutch visual word recognition, we compared lexical decisions to four groups of nonwords in which the existence of ONs and rimes was orthogonally manipulated. Nonwords with existent ONs and/or rimes were rejected more slowly and less accurately. ON and rime neighbours thus influence Dutch nonword reading to the same extent. Simulations with the interactive activation model (McClelland & Rumelhart, 1981) revealed that this model with left-to-right coded representations could not replicate the effects found in the lexical decision data whereas an adapted version with representations of onset, nucleus, and coda could. Effects of the larger units ON and rime emerged from activation patterns created by the smaller units onset, nucleus, and coda.
Phonotactic regularities in the segmentation of spoken Italian
Authors: Tagliapietra Lara , Fanari Rachele , De Candia Chiara , Tabossi Patrizia
Published: May 2008

Five word-spotting experiments explored the role of consonantal and vocalic phonotactic cues in the segmentation of spoken Italian. The first set of experiments tested listeners’ sensitivity to phonotactic constraints cueing syllable boundaries. Participants were slower in spotting words in nonsense strings when target onsets were misaligned (e.g., lago in ri. blago) than when they were aligned (e.g., lago in rin.lago) with phonotactically determined syllabic boundaries. This effect held also for sequences that occur only word-medially (e.g., /tl/ in ri. tlago), and competition effects could not account for the disadvantage in the misaligned condition. Similarly, target detections were slower when their offsets were misaligned (e.g., cittá in cittáu. ba) than when they were aligned (e.g., cittá in cittá.oba) with a phonotactic syllabic boundary. The second set of experiments tested listeners’ sensitivity to phonotactic cues, which specifically signal lexical (and not just syllable) boundaries. Results corroborate the role of syllabic information in speech segmentation and suggest that Italian listeners make little use of additional phonotactic information that specifically cues word boundaries.
Does face familiarity influence speechreadability?
Authors: Lander Karen , Davies Rebecca
Published: May 2008

The impact of face familiarity on the speechreadability of faces is examined. First we measured the baseline speechreading performance of participants, from unfamiliar faces. Next, participants were familiarized with the face and voice of either the same or a different speaker, or were asked to take part in a word puzzle instead. Speechreading performance was measured again, before participants completed a further period of familiarization (or puzzle completion) and a final speechreading performance task. Results showed that speechreading performance increased overall with practice but that performance increased significantly more as participants became increasingly familiar with the same speaker. Our findings demonstrate the importance of talker-specific variations or other instance-based characteristics and suggest that these are a useful source of information for speechreading.
Revisiting the metacontrast dissociation: Comparing sensitivity across different measures and tasks
Authors: Ansorge Ulrich , Becker Stefanie I. , Breitmeyer Bruno
Published: May 2008

In the metacontrast dissociation procedure, presenting a masked shape prime prior to a visible shape target leads to reaction-time effects of the prime in an indirect measure, although participants cannot consciously detect prime shapes in a direct measure (Klotz & Neumann, 1999). This has been taken as evidence for the processing of unconscious input. The results of the present metacontrast dissociation study indicate that although participants are unable to consciously report the shape of the prime, they can consciously perceive motion between masked primes and visible targets in a hybrid direct/indirect measure (Experiments 1 and 3). This indicates that former tests did not provide an exhaustive measure for residual conscious perception of the prime in the metacontrast dissociation procedure. Further tests, however, reveal that residual motion perception cannot account for performance in the indirect measure (Experiments 2 and 3). Although the results thus leave the conception of processing of unconscious input intact, they may prompt a revision of its criteria.
Actions modulate attentional capture
Authors: Welsh Timothy N. , Pratt Jay
Published: May 2008

The purpose of the present study was to determine whether the action system plays a role in determining which stimulus characteristics capture attention by assessing the interference effects caused by dynamic discontinuities that have similar attention-capturing properties, but different action affordances. Results revealed that offset distractors caused significant interference when onset targets were presented in a keypress task, but did not increase reaction times when the same onset targets were presented in a goal-directed aiming task. In contrast, onset distractors caused interference when offset targets were presented in both keypress and aiming tasks. This pattern of results suggests that the attentional set, and thus the properties of stimuli that capture attention, is modulated by the interaction between stimulus and response expectations.
Spatial associations in relational reasoning: Evidence for a SNARC-like effect
Authors: Prado Jérôme , Van Der Henst Jean-Baptiste , Noveck Ira A.
Published: May 2008

Relational reasoning (A > B, B > C, therefore A > C) shares a number of similarities with numerical cognition, including a common behavioural signature, the symbolic distance effect. Just as reaction times for evaluating relational conclusions decrease as the distance between two ordered objects increases, people need less time to compare two numbers when they are distant (e.g., 2 and 8) than when they are close (e.g., 3 and 4). Given that some remain doubtful about such analogical representations in relational reasoning, we determine whether numerical cognition and relational reasoning have other overlapping behavioural effects. Here, using relational reasoning problems that require the alignment of six items, we provide evidence showing that the subjects’ linear mental representation affects motor performance when evaluating conclusions. Items accessible from the left part of a linear representation are evaluated faster when the response is made by the left, rather than the right, hand and the reverse is observed for items accessible from the right part of the linear representation. This effect, observed with the prepositions to the left of and to the right of as well as with above and below, is analogous to the SNARC (Spatial Numerical Association of Response Codes) effect, which is characterized by an interaction between magnitude of numbers and side of response.
Task-defined associations are mode specific for selection of relevant dimension but mode independent for selection of location mapping
Authors: Proctor Robert W. , Vu Kim-Phuong L.
Published: May 2008

When compatible and incompatible mappings of a location-relevant task are mixed, or a location-relevant task is mixed with a task for which stimulus location is irrelevant, the benefit of the compatible mapping is eliminated for physical locations and enhanced for location words. Two experiments examined the influence of presenting the location information for the mixed conditions in different stimulus modes (physical location or word). Experiment 1 showed that the effects of mixing location-relevant and location-irrelevant tasks on the spatial compatibility and Simon effects are reduced when the location information is presented in different modes for the two tasks. Experiment 2 showed, in contrast, that the mode distinction had little influence on the effects of mixed compatible and incompatible mappings for location-relevant tasks: The compatibility effect was eliminated for physical locations and enhanced for words, as when there is no mode distinction. Thus, when location is relevant for one task and colour for the other, the task-defined associations of locations to responses are mode specific, but when location is relevant for both tasks, the associations are mode independent.
Adaptation to Sensory-Motor Temporal Misalignment: Instrumental or Perceptual Learning?
Authors: Kennedy Jon S. , Buehner Marc J. , Rushton Simon K.
Published: May 2008

Sensory-motor delays vary over the course of development and under different environmental conditions. Previous research has shown that humans can compensate for the resulting temporal misalignment while performing sensory-motor tasks (e.g., Cunningham, Billock, & Tsou, 2001a), but remains silent on the question of whether perceptual learning—similar to that involved in adaptation to spatial misalignment (e.g., Redding & Wallace, 1993) and in adaptation to purely intersensory misalignment (e.g., Fujisaki, Shimojo, Kashino, & Nishida, 2004)—is also involved in this adaptive response. Following an attempted replication of Cunningham et al.'s (2001a) study in a preliminary experiment, we present in this paper two experiments that demonstrate that after-effects of adaptation to temporal misalignment do not spontaneously decay. The literature on adaptation to spatial misalignment suggests that, while instrumental learning spontaneously decays in the absence of reinforcement, perceptual learning persists. Therefore our results are consistent with adaptation being effected through perceptual learning.
Numerical representation: Abstract or nonabstract?
Authors: Kadosh Roi Cohen
Published: May 2008

Until now it has been a commonly held view that numbers are represented abstractly in the human brain. However, a recent imaging study challenged the existence of an abstract representation at least of digits and number words, at the brain level, and argued that previous studies and paradigms were not sensitive enough to detect deviations from abstract representation at the behavioural level. The current study addressed this issue with an analysis of distance and sequential effects in magnitude classification. Previous studies that used this paradigm did not find deviation from abstract representation for digits and number words (e.g., Dehaene, 1996; Schwarz & Ischebeck, 2000). However, in the current study a short stimulus–response interval was used, which reduced subjective expectancy and increased automatic processing. The current results showed deviation from abstract representation in both reaction time and accuracy and therefore support the idea that nonabstract representations of numbers do exist.
Understanding causal conditionals: A study of individual differences
Authors: Evans Jonathan St B. T. , Handley Simon J. , Neilens Helen , Over David
Published: May 2008

Previous studies have suggested that a minority of university students, of lower cognitive ability, are inclined to interpret abstract conditional statements, if p then q, as if they were conjunctions: p and q. In the present study we administered the conditional truth table task to a large sample of students ( n = 160), but using realistic, everyday causal conditionals. We also measured their general intelligence. While individual differences were found, these were not consistent with some participants adopting a conjunctive interpretation of such statements. Rather, it appears that students of lower cognitive ability are rather likely to assume that a conditional implies its converse, so that it means also if q then p. The results are discussed with reference to the suppositional theory of conditionals and our more general account of hypothetical thinking.
Learning to Read Words
Authors: Nation Kate
Published: May 2008

The ease with which we process the written word belies its complexities and makes it easy to forget that it is a highly skilled behaviour and one that takes time to master. In this paper, I argue that our ability to read words has its roots in our capacity for language. Good progress has been made towards understanding how children discover the systematic relationship between speech sounds and the letters used to represents those sounds, very early in reading development. However, we understand much less about how beginning readers become skilled readers. To understand this, I argue that it is important to view the visual word recognition system within the context of a broader language system, one that incorporates a rich network of semantic and episodic knowledge.
Short article: The effects of precedence on Navon-induced processing bias in face recognition
Authors: Perfect Timothy J. , Weston Nicola J. , Dennis Ian , Snell Amelia
Published: May 2008

Macrae and Lewis (2002) showed that repeated reporting of the global dimension of Navon stimuli improved performance in a subsequent face identification task, whilst reporting the features of the Navon stimuli impaired performance. Using a face composite task, which is assumed to require featural processing, Weston and Perfect (2005) showed the complementary pattern: Featural responding to Navon letters speeded performance. However, both studies used Navon stimuli with global precedence, in which the overall configuration is easier to report than the features. Here we replicate the two studies above, whilst manipulating the precedence (global or featural) of the letter stimuli in the orientation task. Both studies replicated the previously reported findings with global precedence stimuli, but showed the reverse pattern with local precedence stimuli. These data raise important questions as to what is transferred between the Navon orientation task and the face-processing tasks that follow.
ATF4 is an oxidative stress–inducible, prodeath transcription factor in neurons in vitro and in vivo
Authors: Lange Philipp S. , Chavez Juan C. , Pinto John T. , Coppola Giovanni , Sun Chiao-Wang , Townes Tim M. , Geschwind Daniel H. , Ratan Rajiv R.
Published: May 2008

Oxidative stress is pathogenic in neurological diseases, including stroke. The identity of oxidative stress–inducible transcription factors and their role in propagating the death cascade are not well known. In an in vitro model of oxidative stress, the expression of the bZip transcription factor activating transcription factor 4 (ATF4) was induced by glutathione depletion and localized to the promoter of a putative death gene in neurons. Germline deletion of ATF4 resulted in a profound reduction in oxidative stress–induced gene expression and resistance to oxidative death. In neurons, ATF4 modulates an early, upstream event in the death pathway, as resistance to oxidative death by ATF4 deletion was associated with decreased consumption of the antioxidant glutathione. Forced expression of ATF4 was sufficient to promote cell death and loss of glutathione. In ATF4−/− neurons, restoration of ATF4 protein expression reinstated sensitivity to oxidative death. In addition, ATF4−/− mice experienced significantly smaller infarcts and improved behavioral recovery as compared with wild-type mice subjected to the same reductions in blood flow in a rodent model of ischemic stroke. Collectively, these findings establish ATF4 as a redox-regulated, prodeath transcriptional activator in the nervous system that propagates death responses to oxidative stress in vitro and to stroke in vivo.
Experimental infection of sheep with visna/maedi virus via the conjunctival space
Authors: Niesalla Heide , McNeilly Tom N. , Ross Margaret , Rhind Susan M. , Harkiss Gordon D.
Published: May 2008

Experiments were performed to determine whether visna/maedi virus (VMV), a small ruminant lentivirus (SRLV), could infect sheep via ocular tissues. The EV1 strain of VMV was administered into the conjunctival space of uninfected sheep, and the animals monitored for the presence of provirus DNA and anti-VMV antibodies in blood. The results showed that provirus DNA appeared in peripheral blood mononuclear cells of all animals within a few weeks of receiving either 106TCID50or 103TCID50of VMV. Of the animals receiving the higher dose of virus via the conjunctival space, two seroconverted by 7 and 10 weeks post-infection, one seroconverted 8 months post-infection, and one had not seroconverted by 15 months post-infection. With the lower virus dose, the animals infected via the trachea seroconverted by 4 and 14 weeks, respectively. After ocular infection with this dose, one animal showed a transitory seroconversion with low levels of antibody, peaking at 2 weeks post-administration. The remaining three of the animals infected via the eyes did not seroconvert over a period of 13 months. At post-mortem, evidence for the presence of proviral DNA was obtained from ocular tissue, lungs or mediastinal lymph node in both groups of animals. Histological analysis of lung tissue from animals receiving the lower dose of virus showed the presence of early inflammatory lesions. The results thus show for the first time that transmission of VMV can occur via ocular tissues, suggesting that the conjunctival space may be an additional route of natural transmission.
Genomic and biological characterization of aggressive and docile strains of lymphocytic choriomeningitis virus rescued from a plasmid-based reverse-genetics system
Authors: Chen Minjie , Lan Shuiyun , Ou Rong , Price Graeme E. , Jiang Hong , de la Torre Juan Carlos , Moskophidis Demetrius
Published: May 2008

Arenaviruses include several causative agents of haemorrhagic fever disease in humans. In addition, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a superb model for the study of virus–host interactions, including the basis of viral persistence and associated diseases. There is little understanding about the molecular mechanisms concerning the regulation and specific role of viral proteins in modulating arenavirus–host cell interactions either associated with an acute or persistent infection, and associated disease. Here, we report the genomic and biological characterization of LCMV strains ‘Docile’ (persistent) and ‘Aggressive’ (not persistent) recovered from cloned cDNA via reverse genetics. Our results confirmed that the cloned viruses accurately recreated thein vivophenotypes associated with the corresponding natural Docile and Aggressive viral isolates. In addition, we provide evidence that the ability of the Docile strain to persist is determined by the nature of both S and L RNA segments. Thus, our findings provide the foundation for studies aimed at gaining a detailed understanding of viral determinants of LCMV persistence in its natural host, which may aid in the development of vaccines to prevent or treat the diseases caused by arenaviruses in humans.
Complete sequence characterization of isolates of Getah virus (genus Alphavirus, family Togaviridae) from China
Authors: Zhai You-gang , Wang Huan-Yu , Sun Xiao-hong , Fu Shi-hong , Wang Huan-qin , Attoui Houssam , Tang Qing , Liang Guo-dong
Published: May 2008

Ten virus isolates belonging to species Getah virus (GETV) have been obtained during surveys for arboviruses in China since 1964. Seven of these isolates (YN0540, YN0542, SH05-6, SH05-15, SH05-16, SH05-17 and GS10-2) were obtained during the current study. The full-length sequences of three Chinese isolates (M1, isolated in 1964; HB0234, isolated in 2002; YN0540, isolated in 2005) were determined. The full-length sequences of these isolates were respectively 11 696, 11 686 and 11 690 nt, and showed more than 97 % intraspecies identity. Deletions were found in the capsid protein of strain M1 and non-structural protein nsP3 of strain HB0234. The E2 gene and 3′ UTR of all ten isolates were also characterized. The E2 gene of the Chinese GETV isolates showed nucleotide sequence identities of 98–100 % when compared with other GETV isolates. In the 3′ UTR of the Chinese isolates, an insertion of 10 consecutive adenine residues (nt 189–198) appeared in strain M1, and 9 or 3 consecutive adenines were found towards the 3′ end of the third RES in strains SH05-6 and SH05-15, respectively. The 3′ UTRs of the Chinese isolates showed a deletion between positions 45 and 54 and nucleotide transitions at positions 43, 64 and 148. Sequence and phylogenetic analyses showed that there was a relatively high degree of conservation among GETV isolates. The isolation of GETV from various provinces in China and also in Russia and Mongolia (including regions of the northern tundra) are an indication of changes in the world distribution of this re-emerging virus.
Induction of host defence responses by Drosophila C virus
Authors: Hedges Lauren M. , Johnson Karyn N.
Published: May 2008

Insect responses that are specific for virus infection have been investigated using the genetically tractableDrosophila melanogaster. Most studies focus on interactions withDrosophilaC virus (DCV), which is a member of the familyDicistroviridae. DCV is a non-enveloped,T=3 icosahedral virus with a positive-sense RNA genome. It was demonstrated recently that several genes controlled by the Jak-STAT pathway are specifically upregulated upon DCV infection. To investigate the virus factors that induce these responses, we used the Jak-STAT regulated genes as reporter genes. Challenge of flies with non-infectious DCV particles or double-stranded RNA did not stimulate significant upregulation of the antiviral response genes. In addition, there was no difference in reporter gene upregulation betweenDrosophilachallenged with three different strains of DCV. This suggests that upregulation of theseDrosophilagenes may require virus replication and may involve the non-structural proteins of DCV.
Evaluation of the newly established acne severity classification among Japanese and Korean dermatologists
Authors: HAYASHI Nobukazu , SUH Dae Hun , AKAMATSU Hirohiko , KAWASHIMA Makoto ,   Acne Study Group
Published: May 2008

Recently, we established an acne severity classification that is based on scientific evidence. Our classification allows three different methods for grading, which include general impression of consulted dermatologist, photograph‐based estimation by independent experts, and grading by lesion counting. In our classification, we proposed standard photographs for the estimation of general severity to adjust the basis of judgments. In this study, we evaluated the validity of our classification. We made questionnaires of acne severity using acne patients’ photographs, which were selected from the collection of representative photographs of our classification. Participants answered these questionnaires before and after our presentations about our classification of acne severity. We identified the conformity rate with our consensus decision. The results revealed that average conformity rates were raised from 67.0% to 88.9% among Japanese dermatologists and from 68.0% to 79.8% among Korean ones. These data show the adequacy of both our grading system itself and its presentation. We believe our classification will be one of the most effective and reasonable grading systems to classify acne severity.
Case of pyoderma gangrenosum showing oral and genital ulcers, misdiagnosed as Behcet's disease at first medical examination
Authors: TSUBOI Hiromi
Published: May 2008

It is occasionally difficult to distinguish between Behcet's disease (BD) and pyoderma gangrenous (PG). Our case showed ulcers of the oral, vaginal and perineal areas, and in the ileum, thus resulting in our initial diagnosis of BD. However, the patient showed a continued leukopenia, and she was subsequently diagnosed by bone marrow biopsy as having a myelodysplastic syndrome, which will sometimes accompany PG. In addition, following a hysterectomy, the ulcers of the stump in the vagina and the perineum showed the characteristic findings of a PG‐like destructive ulceration. Based on these findings, we finally diagnosed our case to have PG.
Endothelial hyperplasia and endothelial galectin‐3 expression are prognostic factors in primary central nervous system lymphomas
Authors: D’Haene Nicky , Catteau Xavier , Maris Calliope , Martin Benoit , Salmon Isabelle , Decaestecker Christine
Published: May 2008

Recently, considerable attention has been focused on the identification of clinically relevant prognostic markers for primary central nervous system lymphomas (PCNSL). The present study investigated whether three morphological features, i.e. necrosis, reactive perivascular T‐cell infiltrate and endothelial hyperplasia, and galectin‐1 and galectin‐3 immunohistochemical expression have prognostic roles in a series of 58 PCNSL samples from 44 immunocompetent and 14 immunocompromised patients. The presence of endothelial hyperplasia (identified in 21% of the assessable cases) was identified as a bad prognostic factor for immunocompetent PCNSL patients, whereas the other morphological features were not associated with any prognostic value. Lymphomatous cells of eight PCNSL cases expressed galectin‐3 without any prognostic value, and lymphomatous cells did not express galectin‐1. In contrast, endothelial expression of galectin‐3 was identified (by means of uni‐ and multi‐variate analyses) as a bad prognostic factor for immunocompetent PCNSL patients. In addition, a combination of endothelial hyperplasia and/or endothelial galectin‐3 expression was shown to be an independent prognostic factor for immunocompetent PCNSL patients treated with methotrexate‐based chemotherapy. In summary, this study suggests that endothelial‐related markers can identify risk groups of PCNSL patients and indicates that galectin‐3 could be involved in PCNSL angiogenesis.
Safety and efficacy of cyclophosphamide, adriamycin, vincristine, prednisone and rituximab in patients with human immunodeficiency virus‐associated diffuse large B‐cell lymphoma: results of a phase II trial
Authors: Ribera Josep‐Maria , Oriol Albert , Morgades Mireia , González‐Barca Eva , Miralles Pilar , López‐Guillermo Armando , Gardella Santiago , López Andres , Abella Eugenia , García Marta ,   on behalf of the PETHEMA, GELTAMO, GELCAB and GESIDA Groups
Published: May 2008

Immunochemotherapy with cyclophosphamide, adriamycin, vincristine, prednisone and rituximab (R‐CHOP) is the standard treatment in non‐immunosuppressed patients with diffuse large B‐cell lymphoma (DLBCL), but its adequacy has not been definitively established in patients with human immunodeficiency virus (HIV)‐related lymphoma. This phase II trial aimed to evaluate the safety and efficacy of six cycles of R‐CHOP in patients with HIV‐related DLBCL and to determine whether response to highly active antiretroviral therapy (HAART) had prognostic impact. Patients were eligible if they had performance status <3 and absence of active opportunistic infections. Eighty‐one patients were enrolled, 57 in stages III or IV, International Prognostic Index (IPI) 0 or 1 (n = 26), 2 (n = 19), 3 (n = 20) and 4 or 5 (n = 16), and median CD4 lymphocyte count of 0·158 × 109/l. The main adverse events were neutropenia (48% of cycles) and infections (10% of cycles), which were fatal in seven patients. Complete response was achieved in 55 (69%) patients, with an estimated 3‐year disease‐free survival of 77% and 3‐year overall survival of 56%. IPI score and virological response to HAART were the prognostic parameters for response and survival. In HIV‐related DLBCL R‐CHOP is feasible, safe and effective. The prognosis depends on lymphoma‐related parameters and on the response to HAART.
Intracranial haemorrhage in haemophilia A and B
Authors: Ljung Rolf C. R.
Published: May 2008

In countries with a good standard of health care, intracranial haemorrhage (ICH) during the neonatal period affects 3·5–4·0% of all haemophilia boys, which is considerably (40–80 times) higher than expected in the normal population. ICHs are also frequent after the neonatal period, affecting 3–10% of the haemophilia population who are mainly treated on demand. The risk is higher in inhibitor patients. Spontaneous haemorrhage is reported more frequently than trauma‐induced haemorrhage in most studies. The prevalence of ICH in patients treated with a prophylactic regimen is not known. Although more frequent in younger patients, a substantial proportion of ICH occur in adults, suggesting that general risk factors because of age, such as hypertension, are increasingly important as the haemophiliac gets older. Some studies have reported a substantial proportion of ICH affecting patients with milder forms of haemophilia. The risk of ICH has to be considered when discussing treatment strategies for haemophilia patients.
Protein S deficiency: a clinical perspective
Authors: TEN KATE M. K. , VAN DER MEER J.
Published: May 2008

Summary.  Protein S (PS) is an extensively studied protein with an important function in the down‐regulation of thrombin generation. Because of the presence of a pseudogene and two different forms of PS in plasma, a bound and a free form, it is one of the most difficult thrombophilias to study. A deficiency of PS predisposes subjects to (recurrent) venous thromboembolism (VTE) and foetal loss. However, the conundrum of diagnosing PS deficiency has led to conflicting reports of PS as a risk factor for VTE. In this review, we aim to present a clinical perspective of PS deficiency.