bluuebunny/crossref_metadata_2025 huggingface dataset:
A Hybrid Whale Optimization Algorithm for Global Optimization
Authors: Lee Chun-Yao , Zhuo Guang-Lin
Published: June 2021

This paper proposes a hybrid whale optimization algorithm (WOA) that is derived from the genetic and thermal exchange optimization-based whale optimization algorithm (GWOA-TEO) to enhance global optimization capability. First, the high-quality initial population is generated to improve the performance of GWOA-TEO. Then, thermal exchange optimization (TEO) is applied to improve exploitation performance. Next, a memory is considered that can store historical best-so-far solutions, achieving higher performance without adding additional computational costs. Finally, a crossover operator based on the memory and a position update mechanism of the leading solution based on the memory are proposed to improve the exploration performance. The GWOA-TEO algorithm is then compared with five state-of-the-art optimization algorithms on CEC 2017 benchmark test functions and 8 UCI repository datasets. The statistical results of the CEC 2017 benchmark test functions show that the GWOA-TEO algorithm has good accuracy for global optimization. The classification results of 8 UCI repository datasets also show that the GWOA-TEO algorithm has competitive results with regard to comparison algorithms in recognition rate. Thus, the proposed algorithm is proven to execute excellent performance in solving optimization problems.
Integrated Structure-Control Design of a Bipedal Robot Based on Passive Dynamic Walking
Authors: Martínez-Castelán Josué Nathán , Villarreal-Cervantes Miguel Gabriel
Published: June 2021

The design of bipedal robots is generally fulfilled through considering a sequential design approach, where a synergistic relationship between its structure and control features is not promoted. Hence, a novel integrated structure-control design approach is proposed to simultaneously obtain the optimal structural description, the torque magnitudes, and the on/off time intervals for the control signal input of a semi-passive bipedal robot. The proposed approach takes advantage of the natural dynamics of the system and the control signal activation/deactivation for generating stable gait cycles with minimum energy consumption. Consequently, the passive features of the semi-passive bipedal robot are included in the integrated structure-control design process through evaluating the system behavior along consecutive passive and semi-passive walking stages. Then, the proposed design approach is formulated as a nonlinear discontinuous dynamic optimization problem, where the solution search is carried out using the differential evolution algorithm due to the discontinuities of the semi-passive bipedal robot dynamics. The results of the proposal are compared with those obtained by a sequential design process. The integrated structure-control design achieves a reduction of 63.55% in the value of the performance function related to the synergy between the walking capability and energetic efficiency, with a reduction in the activation of the control and its magnitude of 95.41%.
Special Functions as Solutions to the Euler–Poisson–Darboux Equation with a Fractional Power of the Bessel Operator
Authors: Dzarakhohov Azamat , Luchko Yuri , Shishkina Elina
Published: June 2021

In this paper, we consider fractional ordinary differential equations and the fractional Euler–Poisson–Darboux equation with fractional derivatives in the form of a power of the Bessel differential operator. Using the technique of the Meijer integral transform and its modification, fundamental solutions to these equations are derived in terms of the Fox–Wright function, the Fox H-function, and their particular cases. We also provide some explicit formulas for the solutions to the corresponding initial-value problems in terms of the generalized convolutions introduced in this paper.
A Note on Some Reduction Formulas for the Incomplete Beta Function and the Lerch Transcendent
Authors: González-Santander Juan Luis
Published: June 2021

We derive new reduction formulas for the incomplete beta function Bν,0,z and the Lerch transcendent Φz,1,ν in terms of elementary functions when ν is rational and z is complex. As an application, we calculate some new integrals. Additionally, we use these reduction formulas to test the performance of the algorithms devoted to the numerical evaluation of the incomplete beta function.
A Novel Multi-Criteria Group Decision-Making Approach Based on Bonferroni and Heronian Mean Operators under Hesitant 2-Tuple Linguistic Environment
Authors: Faizi Shahzad , Sałabun Wojciech , Shaheen Nisbha , Rehman Atiq ur , Wątróbski Jarosław
Published: June 2021

Ambiguous and uncertain facts can be handled using a hesitant 2-tuple linguistic set (H2TLS), an important expansion of the 2-tuple linguistic set. The vagueness and uncertainty of data can be grabbed by using aggregation operators. Therefore, aggregation operators play an important role in computational processes to merge the information provided by decision makers (DMs). Furthermore, the aggregation operator is a potential mechanism for merging multisource data which is synonymous with cooperative preference. The aggregation operators need to be studied and analyzed from various perspectives to represent complex choice situations more readily and capture the diverse experiences of DMs. In this manuscript, we propose some valuable operational laws for H2TLS. These new operational laws work through the individual aggregation of linguistic words and the collection of translation parameters. We introduced a hesitant 2-tuple linguistic weighted average (H2TLWA) operator to solve multi-criteria group decision-making (MCGDM) problems. We also define hesitant 2-tuple linguistic Bonferroni mean (H2TLBM) operator, hesitant 2-tuple linguistic geometric Bonferroni mean (H2TLGBM) operator, hesitant 2-tuple linguistic Heronian mean (H2TLHM) operator, and a hesitant 2-tuple linguistic geometric Heronian mean (H2TLGHM) operator based on the novel operational laws proposed in this paper. We define the aggregation operators for addition, subtraction, multiplication, division, scalar multiplication, power and complement with their respective properties. An application example and comparison analysis were examined to show the usefulness and practicality of the work.
Study of Two Families of Generalized Yager’s Implications for Describing the Structure of Generalized (h,e)-Implications
Authors: Fernandez-Peralta Raquel , Massanet Sebastia , Mir Arnau
Published: June 2021

In this study, we analyze the family of generalized (h,e)-implications. We determine when this family fulfills some of the main additional properties of fuzzy implication functions and we obtain a representation theorem that describes the structure of a generalized (h,e)-implication in terms of two families of fuzzy implication functions. These two families can be interpreted as particular cases of the (f,g) and (g,f)-implications, which are two families of fuzzy implication functions that generalize the well-known f and g-generated implications proposed by Yager through a generalization of the internal factors x and 1x, respectively. The behavior and additional properties of these two families are also studied in detail.
Agelasine Diterpenoids and Cbl-b Inhibitory Ageliferins from the Coralline Demosponge Astrosclera willeyana
Authors: Jiang Wei , Wang Dongdong , Wilson Brice , Kang Unwoo , Bokesch Heidi , Smith Emily , Wamiru Antony , Goncharova Ekaterina , Voeller Donna , Lipkowitz Stanley , O’Keefe Barry , Gustafson Kirk
Published: June 2021

An extract of the coralline demosponge Astrosclera willeyana inhibited the ubiquitin ligase activity of the immunomodulatory protein Cbl-b. The bioassay-guided separation of the extract provided ten active compounds, including three new N-methyladenine-containing diterpenoids, agelasines W–Y (1–3), a new bromopyrrole alkaloid, N(1)-methylisoageliferin (4), and six known ageliferin derivatives (5–10). The structures of the new compounds were elucidated from their spectroscopic and spectrometric data, including IR, HRESIMS, and NMR, and by comparison with spectroscopic data in the literature. While all of the isolated compounds showed Cbl-b inhibitory activities, ageliferins (4–10) were the most potent metabolites, with IC50 values that ranged from 18 to 35 μM.
On the Strength of the CF/Al-Wire Depending on the Fabrication Process Parameters: Melt Temperature, Time, Ultrasonic Power, and Thickness of Carbon Fiber Coating
Authors: Galyshev Sergei
Published: June 2021

The process of the production of a CF/Al-wire by pulling carbon fibers through an aluminum melt has at least 15 parameters. The main parameters include the power of ultrasonic treatment, the time of contact of the fiber with the matrix melt, and the melt temperature. In addition, the presence of a barrier coating on the fiber surface and its thickness significantly affects the properties of the resulting material. The importance of these parameters is due to their direct effect on the chemical interaction between the aluminum matrix and the carbon fiber. This interaction leads to the formation of aluminum carbide, a hygroscopic, brittle phase that ultimately significantly reduces the strength of such composites. In this regard, limiting a chemical reaction at the matrix/fiber interface in the production of CF/Al composites is one of the main technological problems. The main goal of this work is to pragmatically elucidate the effect of the above parameters on the strength of CF/Al composites. It is shown that the strength of a CF/Al-wire can reach 2000 MPa.
One-Step Enameling and Sintering of Low-Carbon Steels
Authors: Martinez Miguel Angel , Abenojar Juana , Bahrami Mohsen , Velasco Francisco
Published: June 2021

Powder technology allows manufacturing complex components with small tolerances, saving material without subsequent machining. There is a growing trend in using sintered steel components in the automotive industry. Within 2020, about 2544 million US dollars was invested for manufacturing sintered components. Not only does this industry uses steel components, but the gas cooker industry also uses steel in its burners since they are robust and usually demanded by Americans, with forecasts of 1097 million gas cookers in 2020. Steel gas burners have a ceramic coating on their surface, which means that the burner is manufactured in two stages (casting and enameling). This work aims to manufacture the gas burners by powder metallurgy, enameling and sintering processes in a single step. To achieve this aim, the ASC100.29 iron powder has been characterized (flow rate, relative density and morphology); subsequently, the most suitable parameters for its compaction and an adequate sintering temperature were studied. Single-step sintering and enameling was achieved by compacting iron powder at 500 MPa and sintering at 850 °C for 5 min. The necessary porosity for mechanical anchoring of the coating to the substrate is achieved at this sintering temperature. Bending resistance tests, scratching, degradation under high temperature and basic solution and scanning electron microscopy were used to characterize and validate the obtained samples.
Kinetics of Smelting Chromia–Bearing Vanadiferous Titanomagnetite Ore via High–Temperature CO2–Containing Gas Injection
Authors: Song Hanlin , Cheng Gongjin , Liu Jianxing , Zhang Jinpeng , Xue Xiangxin
Published: June 2021

Through thermodynamic smelting experiments, nonisothermal and isothermal kinetics experiments, the effects of CO2–containing gas injection on the smelting of chromia–bearing vanadiferous titanomagnetite ore were investigated. The experiments at 900 °C, 1000 °C, 1100 °C, 1200 °C, and 1300 °C, and CO2 concentration of 0, 10 vol.%, 20 vol.%, and 30 vol.% were studied. The samples after the kinetics experiments were analyzed through thermodynamic calculations and characterized by XRD, XRF, XPS, and SEM. The results of thermodynamic experiments show that the injection of CO2–containing gas significantly improves the softening–melting–dripping behavior during the smelting process. As the concentration of injected CO2 increased from 0 vol.% to 30 vol.%, the range of softening temperature [T40–T4] decreased from 109 °C to 97 °C, and the range of droplet temperature [Td–Ts] decreased rapidly from 196 °C to 162 °C. Moreover, when CO2 concentration was 20 vol.%, the minimum apparent activation energy of nonisothermal kinetics reached 75.58 kJ·mol−1. Combining the lowest permeability index and the fastest nonisothermal reduction rate, the optimal CO2 concentration in the fuel gas was considered to be 20 vol.%. The isothermal parameters were fitted according to 1 − (1 − α)1/3 − t (CG3 model), and the apparent activation energy was 121.93 kJ·mol−1 (less than 150 kJ·mol−1), which means that the restrictive step of the polymetallic reaction is mainly determined by diffusion. Finally, thermodynamic calculations and characterizations show that CO2–containing gas injection helps titanium stabilize in a higher valence state, which is conducive to improve the high–temperature characteristics of titanium–containing slag.
Structure and Strength of Isothermally Heat-Treated Medium Carbon Ti-V Microalloyed Steel
Authors: Dikić Stefan , Glišić Dragomir , Fadel Abdunnaser , Jovanović Gvozden , Radović Nenad
Published: June 2021

Isothermal transformation characteristics of a medium carbon Ti-V microalloyed steel were investigated using light microscopy, scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS), and by uniaxial compressive testing. Samples austenitized on 1100 °C were isothermally treated in the range from 350 to 600 °C and subsequently water quenched. The final microstructure of the samples held at 350 °C consisted of bainitic sheaves and had compressive yield strength, approximately from 1000 MPa, which is attributed to high dislocation density of low bainite. At 400 and 450 °C, acicular ferrite became prevalent in the microstructure. It was also formed by a displacive mechanism, but the dislocation density was lower, leading to a decrease of compressive yield strength to approximately 700 MPa. The microstructure after the heat treatment at 500 °C consisted of coarse non-polygonal ferrite grains separated by pearlite colonies, principally dislocation free grains, so that the compressive YS reached a minimum value of about 700 MPa. The microstructure of the samples heat-treated at 550 and 600 °C consisted of pearlite and both grain boundary and intragranular ferrite, alongside with some martensite. After 600 s, austenite became stable and transformed to martensite after water quenching. Therefore, the presence of martensite increased the compressive YS to approx. 800 MPa.
Correlating Prior Austenite Grain Microstructure, Microscale Deformation and Fracture of Ultra-High Strength Martensitic Steels
Authors: Zheng Xinzhu , Ghassemi-Armaki Hassan , Hartwig Karl T. , Srivastava Ankit
Published: June 2021

Herein, we correlate the prior austenite grain (PAG) microstructure to deformation and fracture mechanisms of an ultra-high strength martensitic steel. To this end, a low-carbon martensitic steel is subjected to five heat-treatments and the PAG microstructure in the material is reconstructed from the EBSD inverse pole figure maps of the martensitic microstructure. The deformation and fracture response of all heat-treated materials are characterized by in situ tension tests of dog-bone and single-edge notch specimens that allow us to capture both the macroscopic mechanical response and the evolution of microscopic strains via microscale digital image correlation. The experimental results, together with microstructure-based finite element analysis, are then used to elucidate the effect of the PAG microstructure on the mechanical response of the material. Our results show that the interaction between the heterogeneous deformation fields induced by the notch and the bimodal PAG size distribution leads to an increase in the propensity of shear deformation and degradation in the fracture response of the material with increasing heat-treatment temperature and time. Our results also suggest that achieving a unform distribution of fine grains is an effective way to enhance both the strength and fracture properties of this class of materials.
Determination of Critical Transformation Temperatures for the Optimisation of Spring Steel Heat Treatment Processes
Authors: Matjeke Velaphi Jeffrey , van der Merwe Josias Willem , Vithi Nontuthuzelo Lindokuhle
Published: June 2021

Bogie spring performance can be improved by using the exact heat treatment process parameters. The purpose of the study is to determine the critical transformation temperatures and investigate the effect of the cooling rates on microstructural and mechanical properties. The precise determination of the required cooling rates for the particular grade of steel is important in order to optimise the heat treatment process of heavy-duty compression helical spring manufacturing. A traditional heat treatment system for the manufacture of hot coiled springs requires heating the steel to homogenize austenite; then, it is decomposed to martensite by rapid cooling. By analyzing the transition properties by heating and differing cooling rates, this analysis examines the thermal behaviour of high strength spring steel. Using the dilatometer and differential scanning calorimeter, scanning electron microscope, optical microscope, and hardness checking, critical transition temperatures and cooling rates of three springs steels were measured. Although the thermal transformation of materials has been researched for decades using dilatometers, not all materials have been characterized. The research offers insights into the critical transformation temperatures for the defined grades of spring steel and the role of cooling rates in the material’s properties. Mechanical properties are influenced by the transition data obtained from the dilatometric analysis.
A Combined Strategy to Improve the Performance of Dental Alloys Using a New CoCrNbMoZr Alloy with Mn and Si Coated via an Anodic Oxidation Procedure
Authors: Ionascu Florentina Gina , Prodana Mariana , Golgovici Florentina , Demetrescu Ioana
Published: June 2021

The aim of the paper is based on a combined approach to improve dental alloy performance using a new Ni-free Co–Cr composition with Mo, Nb and Zr and coated with an anodic oxidation film. The coated and uncoated samples were surface characterized by performing SEM (scanning electronic microscopy), XRD (X-rays diffraction) contact angle measurements and corrosion studies with open circuit potential, potentiodynamic polarization and EIS (impedance electrochemical spectroscopy) procedures. The SEM equipment with an EDX (Energy-dispersive X-ray spectroscopy) module indicated the sample morphology and the XRD investigations established the formation of the oxides. The electrochemical procedures were performed in Ericsson artificial saliva for coated samples in various conditions. Based on all the experiments, including the decrease in the hydrophobic character of the uncoated samples and the decrease in the hydrophilic values of the anodized alloys, the improved performance of the coated samples was established as a conclusion.
Enhanced Access to the Health-Related Skin Metabolome by Fast, Reproducible and Non-Invasive WET PREP Sampling
Authors: Afghani Jamie , Huelpuesch Claudia , Schmitt-Kopplin Philippe , Traidl-Hoffmann Claudia , Reiger Matthias , Mueller Constanze
Published: June 2021

Our skin influences our physical and mental health, and its chemical composition can reflect environmental and disease conditions. Therefore, through sampling the skin metabolome, we can provide a promising window into the mechanisms of the body. However, the broad application of skin metabolomics has recently been hampered by a lack of easy and widely applicable sampling methods. Here, we present a novel rapid, simple, and, most importantly, painless and non-invasive sampling technique suitable for clinical studies of fragile or weakened skin. The method is called WET PREP and is simply a lavage of the skin which focuses on capturing the metabolome. We systematically evaluate WET PREPs in comparison with the non-invasive method of choice in skin metabolomics, swab collection, using ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-MS2) on two complementary chromatographic columns (C18 reversed phase and hydrophilic interaction chromatography). We also integrate targeted analyses of key metabolites of skin relevance. Overall, WET PREP provides a strikingly more stable shared metabolome across sampled individuals, while also being able to capture unique individual metabolites with a high consistency in intra-individual reproducibility. With the exception of (phospho-)lipidomic studies, we recommend WET PREPs as the preferred skin metabolome sampling technique due to the quick preparation time, low cost, and gentleness for the patient.
Feedborne Salmonella enterica Serovar Jerusalem Outbreak in Different Organic Poultry Flocks in Switzerland and Italy Linked to Soya Expeller
Authors: Horlbog Jule Anna , Stephan Roger , Stevens Marc J. A. , Overesch Gudrun , Kittl Sonja , Napoleoni Maira , Silenzi Valentina , Nüesch-Inderbinen Magdalena , Albini Sarah
Published: June 2021

Poultry feed is a leading source of Salmonella infection in poultry. In Switzerland, heat-treated feed is used to reduce Salmonella incursions into flocks in conventional poultry production. By contrast, organic feed is only treated with organic acids. In 2019, the Swiss National Reference Center for Enteropathogenic Bacteria identified the rare serovar S. Jerusalem from samples of organic soya feed. Further, in July 2020, the European Union’s Rapid Alert System for Food and Feed published a notification of the detection of S. Jerusalem in soya expeller from Italy. During 2020, seven S. Jerusalem isolates from seven different poultry productions distributed over six cantons in Switzerland were reported, providing further evidence of a possible outbreak. Using whole-genome sequencing (WGS), S. Jerusalem isolates from feed and from animals in Switzerland were further characterized and compared to S. Jerusalem from organic poultry farm environments in Italy. WGS results showed that feed isolates and isolates from Swiss and Italian poultry flocks belonged to the sequence type (ST)1028, grouped in a very tight cluster, and were closely related. This outbreak highlights the risk of spreading Salmonella by feed and emphasizes the need for a heat-treatment process for feed, also in organic poultry production.
Insights into Autotrophic Activities and Carbon Flow in Iron-Rich Pelagic Aggregates (Iron Snow)
Authors: Li Qianqian , Cooper Rebecca E. , Wegner Carl-Eric , Taubert Martin , Jehmlich Nico , von Bergen Martin , Küsel Kirsten
Published: June 2021

Pelagic aggregates function as biological carbon pumps for transporting fixed organic carbon to sediments. In iron-rich (ferruginous) lakes, photoferrotrophic and chemolithoautotrophic bacteria contribute to CO2 fixation by oxidizing reduced iron, leading to the formation of iron-rich pelagic aggregates (iron snow). The significance of iron oxidizers in carbon fixation, their general role in iron snow functioning and the flow of carbon within iron snow is still unclear. Here, we combined a two-year metatranscriptome analysis of iron snow collected from an acidic lake with protein-based stable isotope probing to determine general metabolic activities and to trace 13CO2 incorporation in iron snow over time under oxic and anoxic conditions. mRNA-derived metatranscriptome of iron snow identified four key players (Leptospirillum, Ferrovum, Acidithrix, Acidiphilium) with relative abundances (59.6–85.7%) encoding ecologically relevant pathways, including carbon fixation and polysaccharide biosynthesis. No transcriptional activity for carbon fixation from archaea or eukaryotes was detected. 13CO2 incorporation studies identified active chemolithoautotroph Ferrovum under both conditions. Only 1.0–5.3% relative 13C abundances were found in heterotrophic Acidiphilium and Acidocella under oxic conditions. These data show that iron oxidizers play an important role in CO2 fixation, but the majority of fixed C will be directly transported to the sediment without feeding heterotrophs in the water column in acidic ferruginous lakes.
An Observational Prospective Cohort Study of Incidence and Outcome of Streptococcus pneumoniae and Hemophilus influenzae Infections in Adult Solid Organ Transplant Recipients
Authors: Rezahosseini Omid , Møller Dina Leth , Sørensen Søren Schwartz , Perch Michael , Gustafsson Finn , Gelpi Marco , Knudsen Jenny , Helleberg Marie , Rasmussen Allan , Nielsen Susanne Dam , Harboe Zitta Barrella
Published: June 2021

Background: Streptococcus pneumoniae (S. pneumoniae) and Hemophilus influenzae (H. influenzae) are among the main vaccine-preventable bacterial infections in immunocompromised individuals including solid organ transplant (SOT) recipients. There is a lack of information about incidence and outcomes of these infections in SOT recipients. Methods: We determined the incidence of S. pneumoniae and H. influenzae, the related hospitalization, and 30- and 180-days mortality in a large cohort of 1182 adult SOT recipients. We calculated 95% confidence intervals (CI) of incidence rate (IR) using Byar’s approximation to the Poisson distribution. Results: The overall IR of S. pneumoniae and H. influenzae were 1086 (95% CI, 796–1448) and 1293 (95% CI, 974–1687) per 100,000 person-years of follow-up (PYFU), respectively. The IR of invasive infections were 76 (95% CI, 21–202) and 25 (95% CI, 2.3–118) per 100,000 PYFU, respectively. Hospital admission was required in >50%, 30-days mortality was 0, and 180-days mortality was 8.8% and 4.5% after S. pneumoniae and H. influenzae infections, respectively. Conclusions: The IR of invasive S. pneumoniae and H. influenzae infections in SOT recipients were much higher than reports from the general population in Denmark. Furthermore, a large proportion of infected SOT recipients were hospitalized. These findings highlight the need for further studies to assess uptake and immunogenicity of vaccines against S. pneumoniae and H. influenzae in SOT recipients.
Development of a Monocyte Activation Test as an Alternative to the Rabbit Pyrogen Test for Mono- and Multi-Component Shigella GMMA-Based Vaccines
Authors: Carson Danielle , Myhill Sophie , Palmieri Elena , Necchi Francesca , Rijpkema Sjoerd , Micoli Francesca , Nordgren Ida Karin , Rossi Omar , Vipond Caroline
Published: June 2021

Generalised modules for membrane antigens (GMMA)-based vaccines comprise the outer membrane from genetically modified Gram-negative bacteria containing membrane proteins, phospholipids and lipopolysaccharides. Some lipoproteins and lipopolysaccharides are pyrogens; thus, GMMA-based vaccines are intrinsically pyrogenic. It is important to control the pyrogenic content of biological medicines, including vaccines, to prevent adverse reactions such as febrile responses. The rabbit pyrogen test (RPT) and bacterial endotoxin test (BET) are the most commonly employed safety assays used to detect pyrogens. However, both tests are tailored for detecting pyrogenic contaminants and have considerable limitations when measuring the pyrogen content of inherently pyrogenic products. We report the adaptation of the monocyte activation test (MAT) as an alternative to the RPT for monitoring the pyrogenicity of Shigella GMMA-based vaccines. The European Pharmacopoeia endorses three MAT methods (A–C). Of these, method C, the reference lot comparison test, was identified as the most suitable. This method was evaluated with different reference materials to ensure parallelism and consistency for a mono- and multi-component Shigella GMMA vaccine. We demonstrate the drug substance as a promising reference material for safety testing of the matched drug product. Our results support the implementation of MAT as an alternative to the RPT and use of the defined parameters can be extended to GMMA-based vaccines currently in development, aiding vaccine batch release.
DMI-Fungicide Resistance in Venturia nashicola, the Causal Agent of Asian Pear Scab—How Reliable Are Mycelial Growth Tests in Culture?
Authors: Ishii Hideo , Cools Hans Jorgen , Nishimura Kumiko , Borghi Lorenzo , Kikuhara Kenji , Yamaoka Yuichi
Published: June 2021

Scab, caused by Venturia nashicola, is among the most serious diseases of Asian pears and control of this disease largely relies on sterol demethylation inhibitor (DMI) fungicides. However, pear growers have complained about field performance of DMIs since the mid-2000s. In this study, to evaluate pathogen sensitivity, mycelial growth tests and inoculation tests were conducted using DMI-amended culture medium and fungicide-sprayed potted pear trees, respectively. Results confirmed distribution of isolates resistant to fenarimol, hexaconazole, and difenoconazole in the field populations. Importantly, results from tests in culture did not fully correlate with those from tests in planta. Due to phenotypic instability of resistance and poor sporulation of this pathogen in culture, resistance is generally assessed by laborious and time-consuming inoculation with conidia collected from a field. To improve the result interpretation from in vitro tests, the isolates were genotyped: the CYP51 gene which encodes the target sterol 14α-demethylase was sequenced and various mutations have been detected in the coding sequence of DMI-resistant isolates. In addition to the detected single nucleotide polymorphisms, alternative mechanisms, not based on changes in the structure of the target protein, may also increase DMI resistance. Development of molecular methods for the diagnosis of DMI resistance seems to be challenging in V. nashicola.
Effects of Wine and Its Microbial-Derived Metabolites on Intestinal Permeability Using Simulated Gastrointestinal Digestion/Colonic Fermentation and Caco-2 Intestinal Cell Models
Authors: Zorraquín-Peña Irene , Taladrid Diego , Tamargo Alba , Silva Mariana , Molinero Natalia , de Llano Dolores González , Bartolomé Begoña , Moreno-Arribas M. Victoria
Published: June 2021

This paper explores the effects of wine polyphenols on intestinal permeability in in vitro conditions. A red wine (2500 mg/L of gallic acid equivalents) was sequentially subjected to gastrointestinal and colonic digestion in the Dynamic Gastrointestinal Simulator (simgi®) to obtain two simulated fluids: intestinal-digested wine (IDW) and colonic-digested wine (CDW). The two fluids were incubated with Caco-2 cell monolayers grown in Transwell® inserts, and paracellular permeability was measured as transport of FITC-dextran. Non-significant decreases (p > 0.05) in paracellular permeability were found, which was attributed to the relatively low phenolic concentration in the solutions tested (15.6 and 7.8 mg of gallic acid equivalents/L for IDW and CDW, respectively) as quercetin (200 µM) and one of its microbial-derived phenolic metabolites, 3,4-dihydroxyphenylacetic acid (200 µM), led to significant decreases (p < 0.05). The expression of tight junction (TJ) proteins (i.e., ZO-1 and occludin) in Caco-2 cells after incubation with IDW and CDW was also determined. A slight increase in mRNA levels for occludin for both IDW and CDW fluids, albeit without statistical significance (p > 0.05), was observed. Analysis of the microbiome and microbial activity during wine colonic fermentation revealed relevant changes in the relative abundance of some families/genera (i.e., reduction in Bacteroides and an increase in Veillonella, Escherichia/Shigella and Akkermansia) as well as in the microbial production of SCFA (i.e., a significant increase in propionic acid in the presence of IDW), all of which might affect paracellular permeability. Both direct and indirect (microbiota-mediated) mechanisms might be involved in the protective effects of (wine) polyphenols on intestinal barrier integrity. Overall, this paper reinforces (wine) polyphenols as a promising dietary strategy to improve gut functionality, although further studies are needed to evaluate the effect on the intestinal barrier under different conditions.
Antimicrobial Peptide Dendrimers and Quorum-Sensing Inhibitors in Formulating Next-Generation Anti-Infection Cell Therapy Dressings for Burns
Authors: Jafari Paris , Luscher Alexandre , Siriwardena Thissa , Michetti Murielle , Que Yok-Ai , Rahme Laurence G. , Reymond Jean-Louis , Raffoul Wassim , Van Delden Christian , Applegate Lee Ann , Köhler Thilo
Published: June 2021

Multidrug resistance infections are the main cause of failure in the pro-regenerative cell-mediated therapy of burn wounds. The collagen-based matrices for delivery of cells could be potential substrates to support bacterial growth and subsequent lysis of the collagen leading to a cell therapy loss. In this article, we report the development of a new generation of cell therapy formulations with the capacity to resist infections through the bactericidal effect of antimicrobial peptide dendrimers and the anti-virulence effect of anti-quorum sensing MvfR (PqsR) system compounds, which are incorporated into their formulation. Anti-quorum sensing compounds limit the pathogenicity and antibiotic tolerance of pathogenic bacteria involved in the burn wound infections, by inhibiting their virulence pathways. For the first time, we report a biological cell therapy dressing incorporating live progenitor cells, antimicrobial peptide dendrimers, and anti-MvfR compounds, which exhibit bactericidal and anti-virulence properties without compromising the viability of the progenitor cells.
Characterization of Basil Volatile Fraction and Study of Its Agronomic Variation by ASCA
Authors: D’Alessandro Alessandro , Ballestrieri Daniele , Strani Lorenzo , Cocchi Marina , Durante Caterina
Published: June 2021

Basil is a plant known worldwide for its culinary and health attributes. It counts more than a hundred and fifty species and many more chemo-types due to its easy cross-breeds. Each species and each chemo-type have a typical aroma pattern and selecting the proper one is crucial for the food industry. Twelve basil varieties have been studied over three years (2018–2020), as have four different cuts. To characterize the aroma profile, nine typical basil flavour molecules have been selected using a gas chromatography–mass spectrometry coupled with an olfactometer (GC–MS/O). The concentrations of the nine selected molecules were measured by an ultra-fast CG e-nose and Principal Component Analysis (PCA) was applied to detect possible differences among the samples. The PCA results highlighted differences between harvesting years, mainly for 2018, whereas no observable clusters were found concerning varieties and cuts, probably due to the combined effects of the investigated factors. For this reason, the ANOVA Simultaneous Component Analysis (ASCA) methodology was applied on a balanced a posteriori designed dataset. All the considered factors and interactions were statistically significant (p < 0.05) in explaining differences between the basil aroma profiles, with more relevant effects of variety and year.
Characterization of the Aroma Profile and Main Key Odorants of Espresso Coffee
Authors: Angeloni Simone , Mustafa Ahmed M. , Abouelenein Doaa , Alessandroni Laura , Acquaticci Laura , Nzekoue Franks Kamgang , Petrelli Riccardo , Sagratini Gianni , Vittori Sauro , Torregiani Elisabetta , Caprioli Giovanni
Published: June 2021

Espresso coffee (EC) is a common coffee preparation technique that nowadays is broadly widespread all over the globe. Its popularity is in part attributed to the intense aroma and pleasant flavor. Many researchers have studied and reviewed the aroma of the coffee, but there is a lack of specific review focused on EC aroma profile even if it is intensively investigated. Thus, the objective of the current review was to summarize the aroma profile of EC and how different preparation variables can affect EC flavor. Moreover, a collection of diverse analytical procedures for volatile analysis was also reported. The findings of this survey showed that the volatile fraction of EC is extremely complex, but just some compounds are responsible for the characteristic aroma of the coffee, such as some aldehyde, ketones, furanones, furans, sulfur compounds, pyrazines, etc. In addition, during preparation, some variables, e.g., temperature and pressure of water, granulometry of the coffee particle, and brew ratio, can also modify the aroma profile of this beverage, and therefore its quality. A better understanding of the aroma fraction of EC and how the preparation variables should be adjusted according to desired EC would assist coffee workers in obtaining a higher quality product.
The Mechanism of Adsorption of Rh(III) Bromide Complex Ions on Activated Carbon
Authors: Wojnicki Marek , Krawontka Andrzej , Wojtaszek Konrad , Skibińska Katarzyna , Csapó Edit , Pędzich Zbigniew , Podborska Agnieszka , Kwolek Przemysław
Published: June 2021

In the paper, the mechanism of the process of the Rh(III) ions adsorption on activated carbon ORGANOSORB 10—AA was investigated. It was shown, that the process is reversible, i.e., stripping of Rh(III) ions from activated carbon to the solution is also possible. This opens the possibility of industrial recovery of Rh (III) ions from highly dilute aqueous solutions. The activation energies for the forward and backward reaction were determined These are equal to c.a. 7 and 0 kJ/mol. respectively. Unfortunately, the efficiency of this process was low. Obtained maximum load of Rh(III) was equal to 1.13 mg per 1 g of activated carbon.
Diverse Banana Pseudostems and Rachis Are Distinctive for Edible Carbohydrates and Lignocellulose Saccharification towards High Bioethanol Production under Chemical and Liquid Hot Water Pretreatments
Authors: Li Jingyang , Liu Fei , Yu Hua , Li Yuqi , Zhou Shiguang , Ai Yuanhang , Zhou Xinyu , Wang Youmei , Wang Lingqiang , Peng Liangcai , Wang Yanting
Published: June 2021

Banana is a major fruit crop throughout the world with abundant lignocellulose in the pseudostem and rachis residues for biofuel production. In this study, we collected a total of 11 pseudostems and rachis samples that were originally derived from different genetic types and ecological locations of banana crops and then examined largely varied edible carbohydrates (soluble sugars, starch) and lignocellulose compositions. By performing chemical (H2SO4, NaOH) and liquid hot water (LHW) pretreatments, we also found a remarkable variation in biomass enzymatic saccharification and bioethanol production among all banana samples examined. Consequently, this study identified a desirable banana (Refen1, subgroup Pisang Awak) crop containing large amounts of edible carbohydrates and completely digestible lignocellulose, which could be combined to achieve the highest bioethanol yields of 31–38% (% dry matter), compared with previously reported ones in other bioenergy crops. Chemical analysis further indicated that the cellulose CrI and lignin G-monomer should be two major recalcitrant factors affecting biomass enzymatic saccharification in banana pseudostems and rachis. Therefore, this study not only examined rich edible carbohydrates for food in the banana pseudostems but also detected digestible lignocellulose for bioethanol production in rachis tissue, providing a strategy applicable for genetic breeding and biomass processing in banana crops.
A Deep Learning Approach for Molecular Classification Based on AFM Images
Authors: Carracedo-Cosme Jaime , Romero-Muñiz Carlos , Pérez Rubén
Published: June 2021

In spite of the unprecedented resolution provided by non-contact atomic force microscopy (AFM) with CO-functionalized and advances in the interpretation of the observed contrast, the unambiguous identification of molecular systems solely based on AFM images, without any prior information, remains an open problem. This work presents a first step towards the automatic classification of AFM experimental images by a deep learning model trained essentially with a theoretically generated dataset. We analyze the limitations of two standard models for pattern recognition when applied to AFM image classification and develop a model with the optimal depth to provide accurate results and to retain the ability to generalize. We show that a variational autoencoder (VAE) provides a very efficient way to incorporate, from very few experimental images, characteristic features into the training set that assure a high accuracy in the classification of both theoretical and experimental images.
Complete Degradation and Detoxification of Ciprofloxacin by a Micro-/Nanostructured Biogenic Mn Oxide Composite from a Highly Active Mn2+-Oxidizing Pseudomonas Strain
Authors: Li Li , Liu Jin , Zeng Jie , Li Jiaoqing , Liu Yongxuan , Sun Xiaowen , Xu Liangzheng , Li Lin
Published: June 2021

Ciprofloxacin (CIP), as a representative broad-spectrum antibiotic, poses a major threat to human health and the ecological environment as a result of its abuse and emissions. In this study, a highly active Mn2+-oxidizing bacterium, Pseudomonas sp. CCTCC M2014168, was induced to form micro-/nanostructured biogenic Mn oxide (BMO) aggregates through continuous culturing with 1 mmoL−1 Mn2+. Following the characterization of Mn4+ oxides and the micro-/nanostructures by scanning electron microscopy, high-resolution transmission electron microscopy and X-ray diffraction assays, the BMO composites were subjected to CIP degradation and detoxification in laboratory trials. High-performance liquid chromatograph (HPLC) analysis identified that the BMO composites were capable of completely degrading CIP, and HPLC with a mass spectrometer (LC/MS) assays identified three intermediates in the degradation pathway. The reaction temperature, pH and initial ciprofloxacin concentration substantially affected the degradation efficiency of CIP to a certain extent, and the metal ions Mg2+, Cu2+, Ni2+ and Co2+ exerted significant inhibitory effects on CIP degradation. A toxicity test of the degradation products showed that CIP was completely detoxified by degradation. Moreover, the prepared BMO composite exhibited a high capacity for repeated degradation and good performance in continuous degradation cycles, as well as a high capacity to degrade CIP in real natural water.
Combining Augmented Radiotherapy and Immunotherapy through a Nano-Gold and Bacterial Outer-Membrane Vesicle Complex for the Treatment of Glioblastoma
Authors: Chen Mei-Hsiu , Liu Tse-Ying , Chen Yu-Chiao , Chen Ming-Hong
Published: June 2021

Glioblastoma, formerly known as glioblastoma multiforme (GBM), is refractory to existing adjuvant chemotherapy and radiotherapy. We successfully synthesized a complex, Au–OMV, with two specific nanoparticles: gold nanoparticles (AuNPs) and outer-membrane vesicles (OMVs) from E. coli. Au–OMV, when combined with radiotherapy, produced radiosensitizing and immuno-modulatory effects that successfully suppressed tumor growth in both subcutaneous G261 tumor-bearing and in situ (brain) tumor-bearing C57BL/6 mice. Longer survival was also noted with in situ tumor-bearing mice treated with Au–OMV and radiotherapy. The mechanisms for the successful treatment were evaluated. Intracellular reactive oxygen species (ROS) greatly increased in response to Au–OMV in combination with radiotherapy in G261 glioma cells. Furthermore, with a co-culture of G261 glioma cells and RAW 264.7 macrophages, we found that GL261 cell viability was related to chemotaxis of macrophages and TNF-α production.
Standing and Lying Ni(OH)2 Nanosheets on Multilayer Graphene for High-Performance Supercapacitors
Authors: Xu Junming , Tang Mengxia , Hu Zhengming , Hu Xiaoping , Zhou Tao , Song Kaixin , Wu Jun , Cheng Jipeng
Published: June 2021

For conventional synthesis of Ni(OH)2/graphene hybrids, oxygen-containing functional groups should be firstly introduced on graphene to serve as active sites for the anchoring of Ni(OH)2. In this work, a method for growing Ni(OH)2 nanosheets on multilayer graphene (MLG) with molecular connection is developed which does not need any pre-activation treatments. Moreover, Ni(OH)2 nanosheets can be controlled to stand or lie on the surface of MLG. The prepared hybrids were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS). The growth processes are suggested according to their morphologies at different growth stages. The enhanced electrochemical performances as supercapacitor electrode materials were confirmed by cyclic voltammetry (CV) and galvanostatic charge-discharge (GCD) techniques. Ni(OH)2 nanosheets standing and lying on MLG show specific capacities of 204.4 mAh g−1 and 131.7 mAh g−1, respectively, at 1 A g−1 based on the total mass of the hybrids and 81.5% and 92.8% capacity retention at a high current density of 10 A g−1, respectively. Hybrid supercapacitors with as-prepared hybrids as cathodes and activated carbon as anode were fabricated and tested.
Silicone Nanofilament Support Layers in an Open-Channel System for the Fast Reduction of Para-Nitrophenol
Authors: Naef Noah U. , Seeger Stefan
Published: June 2021

Chemical vapor phase deposition was used to create hydrophobic nanostructured surfaces on glass slides. Subsequently, hydrophilic channels were created by sputtering a metal catalyst on the channels while masking the outside. The surface tension gradient between the hydrophilic surface in the channels and the outside hydrophobicity formed the open-channel system. The reduction of para-nitrophenol (PNP) was studied on these devices. When compared to nanostructure-free reference systems, the created nanostructures, namely, silicone nanofilaments (SNFs) and nano-bagels, had superior catalytic performance (73% and 66% conversion to 55% at 0.5 µL/s flow rate using 20 nm platinum) and wall integrity; therefore, they could be readily used multiple times. The created nanostructures were stable under the reaction conditions, as observed with scanning electron microscopy. Transition electron microscopy studies of platinum-modified SNFs revealed that the catalyst is present as nanoparticles ranging up to 13 nm in size. By changing the target in the sputter coating unit, molybdenum, gold, nickel and copper were evaluated for their catalytic efficiency. The relative order was platinum < gold = molybdenum < nickel < copper. The decomposition of sodium borohydride (NaBH4) by platinum as a concurrent reaction to the para-nitrophenol reduction terminates the reaction before completion, despite a large excess of reducing agent. Gold had the same catalytic rate as molybdenum, while nickel was two times and copper about four times faster than gold. In all cases, there was a clear improvement in catalysis of silicone nanofilaments compared to a flat reference system.