bluuebunny/crossref_metadata_2025 huggingface dataset:
Benefits of Insect Pollination in Brassicaceae: A Meta-Analysis of Self-Compatible and Self-Incompatible Crop Species
Authors: Badenes-Pérez Francisco Rubén
Published: March 2022

This paper reviewed the effects of insect pollination on the yield parameters of plants from the family Brassicaceae presenting different breeding systems. Meta-analysis indicates that in both self-compatible and self-incompatible crop species, meta-analysis indicates that seed yield (Y), silique set (SQS), number of siliquae/plant (NSQ), and the number of seeds/silique (NSSQ) increase when plants are insect-pollinated compared to when there is no insect pollination. The weight of seeds (WS), however, increased in self-incompatible species but not in self-compatible ones as a result of insect pollination. Overall, the percentage of studies showing a positive effect of insect pollination on yield parameters was higher in self-incompatible than in self-compatible species. It was shown that the ability of self-compatible species to reproduce does not fully compensate for the loss of yield benefits in the absence of insect pollination. Cultivated Brassicaceae attract a wide variety of pollinators, with honeybees (Apis spp.) such as A. mellifera L., A. cerana F., A. dorsata F., and A. florea F. (Hymenoptera: Apidae); other Apidae, such as bumblebees (Bombus spp.) (Hymenoptera: Apidae); mining bees (Hymenoptera: Andrenidae); sweat bees (Hymenoptera: Halictidae); and hoverflies (Diptera: Syrphidae) constituting the most common ones. The benefits of insect pollination imply that pollinator conservation programs play a key role in maximizing yield in cruciferous crops.
Catch Crops in Lower Saxony—More Than 30 Years of Action against Water Pollution with Nitrates: All in Vain?
Authors: Klages Susanne , Aue Christina , Reiter Karin , Heidecke Claudia , Osterburg Bernhard
Published: March 2022

Intensive animal production, vast amounts of biogas plants, and the spreading of manure and digestates, exerts strong pressure on water quality in the German federal state of Lower Saxony. Catch and cover crop (c&c) cultivation is seen as one measure to inhibit nitrate leaching into soils, and to prevent water pollution with nitrates. A document analysis was carried out, covering the time span of 1992 to 2020, and the findings were combined with available quantitative data of the same period, and with GIS analysis. From 1994 to the year 2020, the acreage of subsidized c&cs increased from ca. 10,000 ha to ca. 380,000 ha. In addition, there was an acreage of unsubsidized c&cs of about 100,000 ha declining to 50,000 ha. In comparison, the acreage of arable land remained at approximately 1,880,000 ha. We found that c&cs did not contribute substantially to water protection for the following reasons: the design of the measure, control of farmer’s actions, and the antagonistic trend due to the increase in animal numbers and biogas plants. The development of c&cs over time and space reveals that frame conditions and management requirements of cultivating c&cs need to be well designed to be effective and efficient (with regard to N reduction and reduction of costs). It is vital to coordinate all programs and schemes in one region. From our evaluation, we conclude that a measure such as c&c cultivation, which is simple to introduce and easy to control, should be implemented over winter as a mandatory measure in order to achieve a greater uptake. Additionally, result-based measures could complement this scheme, as there is a strong link between subsidy level and the success of the measure.
Decision Pattern for Changing Polluted Areas into Recreational Places
Authors: Parra Alfonso , Conesa Encarnación , Zornoza Raúl , Faz Ángel , Gómez-López María Dolores
Published: March 2022

There are many enclaves in the Mediterranean basin with soils contaminated with heavy metal(loid)s, most of these in natural areas of great beauty that have suffered the consequences of industrial and mining activity for years. These soils pose a risk to human and animal health due to the transfer of metal(loid)s condemning these areas to isolation. The rehabilitation by means of phytoremediation is one of the most used techniques, but phytoremediation must be part of a comprehensive strategy of steps that guide owners and administrations in the recovery of ecosystem services. An easily replicable decision-making methodology is defined, considering the initial conditions, the preferences of the decision makers or typologies from among six possibilities and the different models of use, typified in 13 categories. As a result, a landscape is obtained that integrates phytostabilization and areas with recreational and/or educational uses. Two case studies from the southeast of Spain are presented as validation, a deposit of mining sludge residues and the channel of a river contaminated by industrial discharges. Both enclaves are included in the tourist and cultural offer of their area, thus achieving an environmental and socioeconomic benefit and have been visited by more than 1000 people in a two-year period.
Chronic Intestinal Disorders in Humans and Pets: Current Management and the Potential of Nutraceutical Antioxidants as Alternatives
Authors: Meineri Giorgia , Martello Elisa , Radice Elisabetta , Bruni Natascia , Saettone Vittorio , Atuahene David , Armandi Angelo , Testa Giulia , Ribaldone Davide Giuseppe
Published: March 2022

Chronic intestinal disorders (CID) are characterized by persistent, or recurrent gastrointestinal (GI) signs present for at least three weeks. In human medicine, inflammatory bowel disease (IBD) is a group of chronic GI diseases and includes Crohn’s disease (CD) and ulcerative colitis (UC). On the other hand, the general term chronic enteropathies (CE) is preferred in veterinary medicine. Different therapeutic approaches to these diseases are used in both humans and pets. This review is focused on the use of traditional therapies and nutraceuticals with specific antioxidant properties, for the treatment of CID in humans and animal patients. There is strong evidence of the antioxidant properties of the nutraceuticals included in this review, but few studies report their use for treating CID in humans and none in animals. Despite this fact, the majority of the nutraceuticals described in the present article could be considered as promising alternatives for the regular treatment of CID in human and veterinary medicine.
Infrared Predictions Are a Valuable Alternative to Actual Measures of Dry-Cured Ham Weight Loss in the Training of Genome-Enabled Prediction Models
Authors: Bonfatti Valentina , Faggion Sara , Boschi Elena , Carnier Paolo
Published: March 2022

Selection to reduce ham weight losses during dry-curing (WL) requires individual traceability of hams throughout dry-curing, with high phenotyping costs and long generation intervals. Infrared spectroscopy enables cost-effective, high-throughput phenotyping for WL 24 h after slaughter. Direct genomic values (DGV) of crossbred pigs and their purebred sires were estimated, for observed (OB) and infrared-predicted WL (IR), through models developed from 640 and 956 crossbred pigs, respectively. Five Bayesian models and two pseudo-phenotypes (estimated breeding value, EBV, and adjusted phenotype) were tested in random cross-validation and leave-one-family-out validation. The use of EBV as pseudo-phenotypes resulted in the highest accuracies. Accuracies in leave-one-family-out validation were much lower than those obtained in random cross-validation but still satisfactory and very similar for both traits. For sires in the leave-one-family-out validation scenario, the correlation between the DGV for IR and EBV for OB was slightly lower (0.32) than the correlation between the DGV for OB and EBV for OB (0.38). While genomic prediction of OB and IR can be equally suggested to be incorporated in future selection programs aiming at reducing WL, the use of IR enables an early, cost-effective phenotyping, favoring the construction of larger reference populations, with accuracies comparable to those achievable using OB phenotype.
Effects of Corn and Broken Rice Extrusion on the Feed Intake, Nutrient Digestibility, and Gut Microbiota of Weaned Piglets
Authors: Zhuo Yong , Huang Yingyan , He Jiaqi , Hua Lun , Xu Shengyu , Li Jian , Che Lianqiang , Lin Yan , Feng Bin , Wu De
Published: March 2022

In this study, we investigated the effects of corn and rice extrusion diets on feed intake, nutrient digestibility, and gut microbiota in weaned piglets. Animals were divided into four dietary groups and fed a controlled diet containing (1) 62.17% corn (CORN), 15% soybean, 10% extruded full-fat soybean, and 6% fishmeal (2) half the corn replaced by extruded corn (ECORN), (3) broken rice (RICE), and (4) extruded broken rice (ERICE) for 28 days. Rice supplementation increased dry matter total tract digestibility and gross energy. Extruded cereals generated a lower average daily feed intake (ADFI) at 15–28 and 1–28 days, decreased average daily growth (ADG) at 15–28 and 1–28 days, and a lowered body weight (BW) on day 28, regardless of cereal type. Dietary extruded cereals increased the appetite-regulating hormones glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). Piglets fed extruded cereals displayed low short-chain fatty acid (SCFA) levels in plasma and low Lactobaillaceae and Bifidobateriaceae levels in feces, whereas a higher abundance of the potential pathogens Sarcina, Clostridium_sensu_strictio_1 and Terrisporobacter was observed. Piglets fed extruded cereals displayed significantly lower gas and SCFA levels during in vitro fermentation. Combined, 50% corn substituted with extruded corn or broken rice decreased piglet growth performance, possibly by altering their microbiota.
Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods
Authors: Gajic Ina , Kabic Jovana , Kekic Dusan , Jovicevic Milos , Milenkovic Marina , Mitic Culafic Dragana , Trudic Anika , Ranin Lazar , Opavski Natasa
Published: March 2022

Antimicrobial resistance (AMR) has emerged as a major threat to public health globally. Accurate and rapid detection of resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment, combined with antimicrobial stewardship, are essential for controlling the emergence and spread of AMR. This article reviews common antimicrobial susceptibility testing (AST) methods and relevant issues concerning the advantages and disadvantages of each method. Although accurate, classic technologies used in clinical microbiology to profile antimicrobial susceptibility are time-consuming and relatively expensive. As a result, physicians often prescribe empirical antimicrobial therapies and broad-spectrum antibiotics. Although recently developed AST systems have shown advantages over traditional methods in terms of testing speed and the potential for providing a deeper insight into resistance mechanisms, extensive validation is required to translate these methodologies to clinical practice. With a continuous increase in antimicrobial resistance, additional efforts are needed to develop innovative, rapid, accurate, and portable diagnostic tools for AST. The wide implementation of novel devices would enable the identification of the optimal treatment approaches and the surveillance of antibiotic resistance in health, agriculture, and the environment, allowing monitoring and better tackling the emergence of AMR.
Exosomes from Human Placenta Choriodecidual Membrane-Derived Mesenchymal Stem Cells Mitigate Endoplasmic Reticulum Stress, Inflammation, and Lung Injury in Lipopolysaccharide-Treated Obese Mice
Authors: Chiang Milton D. , Chang Chao-Yuan , Shih Hung-Jen , Le Van Long , Huang Yen-Hua , Huang Chun-Jen
Published: March 2022

Endoplasmic reticulum (ER) stress mediates the effects of obesity on aggravating sepsis-induced lung injury. We investigated whether exosomes from human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) can mitigate pulmonary ER stress, lung injury, and the mechanisms of inflammation, oxidation, and apoptosis in lipopolysaccharide-treated obese mice. Diet-induced obese (DIO) mice (adult male C57BL/6J mice fed with a 12-week high-fat diet) received lipopolysaccharide (10 mg/kg, i.p.; DIOLPS group) or lipopolysaccharide plus exosomes (1 × 108 particles/mouse, i.p.; DIOLPSExo group). Our data demonstrated lower levels of ER stress (upregulation of glucose-regulated protein 78, phosphorylated eukaryotic initiation factor 2α, and C/EBP homologous protein; p = 0.038, <0.001, and <0.001, respectively), inflammation (activation of nuclear factor-kB, hypoxia-inducible factor-1α, macrophages, and NLR family pyrin domain containing 3; upregulation of tumor necrosis factor-α, interleukin-1β, and interleukin-6; p = 0.03, <0.001, <0.001, <0.001, <0.001, <0.001, and <0.001, respectively), lipid peroxidation (p < 0.001), and apoptosis (DNA fragmentation, p = 0.003) in lung tissues, as well as lower lung injury level (decreases in tidal volume, peak inspiratory flow, and end expiratory volume; increases in resistance, injury score, and tissue water content; p < 0.001, <0.001, <0.001, <0.001, <0.001, and =0.002, respectively) in the DIOLPSExo group than in the DIOLPS group. In conclusion, exosomes from human pcMSCs mitigate pulmonary ER stress, inflammation, oxidation, apoptosis, and lung injury in lipopolysaccharide-treated obese mice.
Blueberry Polyphenols Increase Nitric Oxide and Attenuate Angiotensin II-Induced Oxidative Stress and Inflammatory Signaling in Human Aortic Endothelial Cells
Authors: Najjar Rami S. , Mu Shengyu , Feresin Rafaela G.
Published: March 2022

Accumulating evidence indicate that blueberries have anti-hypertensive properties, which may be mainly due to its rich polyphenol content and their high antioxidant capacity. Thus, we aimed to investigate the mechanisms by which blueberry polyphenols exert these effects. Human aortic endothelial cells (HAECs) were incubated with 200 µg/mL blueberry polyphenol extract (BPE) for 1 h prior to a 12 h treatment with angiotensin (Ang) II, a potent vasoconstrictor. Our results indicate that Ang II increased levels of superoxide anions and decreased NO levels in HAECs. These effects were attenuated by pre-treatment with BPE. Ang II increased the expression of the pro-oxidant enzyme NOX1, which was not attenuated by BPE. Pre-treatment with BPE attenuated the Ang II-induced increase in the phosphorylation of the redox-sensitive MAPK kinases, SAPK/JNK and p38. BPE increased the expression of the redox-transcription factor NRF2 as well as detoxifying and antioxidant enzymes it transcribes including HO-1, NQO1, and SOD1. We also show that BPE attenuates the Ang II-induced phosphorylation of the NF-κB p65 subunit. Further, we show that inhibition of NRF2 leads to a decrease in the expression of HO-1 and increased phosphorylation of the NF-κB p65 subunit in HAECs treated with BPE and Ang II. These findings indicate that BPE acts through a NRF2-dependent mechanism to reduce oxidative stress and increase NO levels in Ang II-treated HAECs.
A New Approach to Modeling Focused Infrared Heating Based on Quantum Mechanical Formulations
Authors: Oh Sung-Hyun , Lee Eun-Ho
Published: March 2022

The focused infrared (IR) heating method is an energy-efficient heating technology for engineering applications. Numerical models of focused IR heating technology have been introduced based on the theory of ray optics. The ray optics-based IR models have provided good simulation results; however, they are mathematically complex because the ray optics models need to account for the complex paths of the IR rays and the geometrical information of the heating devices. This paper presents a new approach for modeling the focused IR heating method using quantum mechanical formulations. Even though the IR heating condition is not a pure quantum phenomenon, it is efficient to employ the concept of the superposition principle of wave functions in IR distribution modeling. The proposed model makes an abstraction by replacing the distributed IR rays with an energy particle with independent wave functions at different eigenstates, based on the Schrödinger equation. The new approach results in a simpler equation for modeling the focused IR heating method. An electrical-thermal simulation of the focused IR heating with the new model provides results in good agreement with the experimental data.
Design of an Emulgel for Psoriasis Focused on Patient Preferences
Authors: Oliveira Rita Sanches , da Silva Diva Ferraz , Mota Sandra , Garrido Jorge , Garrido Ermelinda Manuela , Lobo José Manuel Sousa , Almeida Isabel Filipa
Published: March 2022

Adherence to topical treatments is low and is known to be influenced by the vehicle properties. Betamethasone dipropionate (BD) is an anti-inflammatory steroid, used in psoriasis treatment in the form of an ointment, cream, or solution. The aim of this work was to develop a new vehicle for BD, focusing on the preferences of patients with psoriasis as a strategy to improve treatment adherence. Two vehicles with an aqueous external phase were explored: an emulgel and a hydrogel based on a cyclodextrin inclusion complex used to improve the aqueous solubility of BD. Since BD solubilization was not fully achieved in the hydrogel, only the emulgel was selected for further characterization. This new vehicle (emulgel) is characterized by its white, shiny appearance and good spreading properties. In comparison with petrolatum, a lower residue, higher evaporation rate, lower stickiness, and reduced ability to stain polyester fabric were observed. This vehicle also showed shear thinning behavior. The impact of this new vehicle on adherence to topical treatments should be further confirmed in clinical settings.
Morphological, Histological and Ultrastructural Changes in Hordeum vulgare (L.) Roots That Have Been Exposed to Negatively Charged Gold Nanoparticles
Authors: Milewska-Hendel Anna , Gepfert Weronika , Zubko Maciej , Kurczyńska Ewa
Published: March 2022

In recent years, there has been an impressive development of nanotechnology. This has resulted in the increasing release of nanomaterials (NM) into the environment, thereby causing the risk of an uncontrolled impact on living organisms, including plants. More studies indicated the biotoxic effect of NM on plants, including crops. The interaction of nanoparticles (NP) with food crops is extremely important as they are a link to the food chain. The objective of this study was to investigate the effect of negatively charged gold nanoparticles (-) AuNP (at two concentrations; 25 µg/mL or 50 µg/mL) on barley (Hordeum vulgare L.) root development. Morphological, histological and ultrastructural analyses (with the use of stereomicroscope, bright filed microscope and transmission electron microscope) revealed that regardless of the concentration, (-) AuNP did not enter into the plant body. However, the dose of (-) AuNP proved to be important for the plant’s response because different morphological, histological and ultrastructural changes were observed in the treated roots. The NP treatment caused: red root colouration, a local increase in the root diameter and a decreased formation of the root hair cells (on morphological level), damage to the rhizodermal cells, vacuolisation of the cortical cells, a detachment of the cell files between the cortical cells, atypical divisions of the cells, disorder of the meristem organisation (on the histological level), the appearance of periplasmic space, numerous vesicles and multivesicular bodies, electron-dense spots in cytoplasm, alterations in the structure of the mitochondria, breakdown of the tonoplast and the plasmalemma (on the ultrastructural level).
An Optimizing Heat Consumption System Based on BMS
Authors: Gaitan Nicoleta Cristina , Ungurean Ioan , Roman Costica , Francu Catalin
Published: March 2022

The recent trend is to transform existing buildings into smart, green, or passive buildings by adopting sustainable technologies to achieve increased life comfort and production efficiency through reducing carbon emissions, energy consumption, and operating costs. Thus, existing buildings can be rehabilitated in order to have the lowest possible energy consumption. One of the highest demands on the energy consumption of buildings is the heating system used during the winter months. In this paper, we propose a management and control system for electrical and energy heating consumption, based on a BMS (building management system) that is used for the rehabilitation of the students’ dormitories on a university campus. This includes a cogeneration plant that can be controlled in order to produce electrical and heating energy, depending on the requirements needed to heat the building in the cold season. The system reduced the consumption of energy for heating by 13% and of electricity by 32%.
Structuring a BIM Service Scoping, Tendering, Executing, and Wrapping-Up (STEW) Guide for Public Owners
Authors: Park Kyoung-Jun , Ock Jong-Ho
Published: March 2022

Building Information Modeling (BIM) has been one of the most dominant trends in the construction industry worldwide. In Korea, since 2012, public construction projects worth more than USD 40 million have been subject to the application of BIM. However, many public owners have hesitated to adopt BIM for their projects; they generally view BIM as being very complex to manage and difficult to understand. In 2021, the Korean governmental entity in charge of developing the public policies to furnish quality infrastructure requested a variety of individual public owners to prepare BIM guidelines fit to the infrastructures they manage. While there are diverse BIM guides available for them to refer to in preparing the BIM guidelines, these involve certain deficiencies in meeting the public owners’ preferences, such as being tailored for the public procurement process. The main objectives of this study were to investigate the desirable nature of an owner-oriented BIM guide, suggest the structure of the guide, and identify the major advisory contents to be included in the guide. To achieve these research objectives, a number of domestic and international BIM guides were analyzed, and workshops were operated twice with 12 BIM experts from the Korean construction industry. As a result of the study, a four-stage guide structure was developed consisting of the BIM service Scoping, Tendering, Executing, and Wrapping-up (STEW) guides.
A Survey of IoT-Based Fall Detection for Aiding Elderly Care: Sensors, Methods, Challenges and Future Trends
Authors: Karar Mohamed Esmail , Shehata Hazem Ibrahim , Reyad Omar
Published: March 2022

Remote monitoring of a fall condition or activities and daily life (ADL) of elderly patients has become one of the essential purposes for modern telemedicine. Internet of Things (IoT) and artificial intelligence (AI) techniques, including machine and deep learning models, have been recently applied in the medical field to automate the diagnosis procedures of abnormal and diseased cases. They also have many other applications, including the real-time identification of fall accidents in elderly patients. The goal of this article is to review recent research whose focus is to develop AI algorithms and methods of fall detection systems (FDS) in the IoT environment. In addition, the usability of different sensor types, such as gyroscopes and accelerometers in smartwatches, is described and discussed with the current limitations and challenges for realizing successful FDSs. The availability problem of public fall datasets for evaluating the proposed detection algorithms are also addressed in this study. Finally, this article is concluded by proposing advanced techniques such as lightweight deep models as one of the solutions and prospects of futuristic smart IoT-enabled systems for accurate fall detection in the elderly.
Safety and Efficacy of Modern Stents in Patients with Metabolic Syndrome
Authors: Pașc Priscilla , Dobjanschi Luciana , Zdrîncă Mihaela , Zdrîncă Marcel , Ștefan Liana , Huniadi Carmen Anca , Lestyan Marieta , Miere (Groza) Florina , Popescu Mircea Ioachim
Published: March 2022

The impact of metabolic syndrome (MS) on stent performance and outcomes of patients underwent percutaneous coronary intervention (PCI), including stent implantation, had not yet been fully established. The aim of the present study was to investigate long-term safety and efficacy of 2nd generation DES vs. other stent types in MS patients underwent successful PCI for an acute coronary event. Descriptive epidemiology, angiographic characteristics and one-year clinical outcomes, including major adverse cardiovascular events (MACE) and target lesion revascularization (TLR) procedures were evaluated according to the stent type inserted for 204 patients. The occurrence of TLR (6.62% vs. 9.43%, p = 0.50) and the composite outcomes (20.52% vs. 24.52%, p = 0.54) did not differ significantly between patients having 2nd generation drug eluting stents (DES) or other stent types implanted at the index procedure. It seems that the PCI in acute coronary syndrome (ACS) is similarly efficient regardless of the stent type, suggesting that for the treatment of ACS in MS patients, all stent types show similar results, in terms of TLR or MACE.
Anti-Allergic Effects of Fermented Red Ginseng Marc on 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-like Mice Model
Authors: Jung Yeun Soo , Choi Jae Young , Kwon Young-Sam , Park Gyu-Ryeul , Dachuri VinayKumar , Kim Young Woo , Ku Sae-Kwang , Song Chang-Hyun
Published: March 2022

Atopic dermatitis (AD) is a chronic and allergic skin disease; however, there is no cure for the disease. Red ginseng is well known to have anti-AD potential, while red ginseng marc (RGM) remaining after ginseng extraction is regarded as useless and discarded. However, it has recently been reported that RGM, particularly fermented RGM (fRGM), still contains bioactive properties. Thus, the anti-allergic effects of fRGM were examined in a 2,4-dinitrochlorobenzene-induced AD-like mice model. The model was topically treated with distilled water (control), dexamethasone, or fRGM for six weeks. Treatments of fRGM alleviated skin lesions and reduced serum IgE levels, compared with the control. The fRGM also reduced skin levels of lipid peroxidation and superoxide anion; however, it increased glutathione contents, with downregulated gene expression for inflammatory mediators. Histopathological analyses demonstrated that fRGM suppressed epidermal thickening, collagen deposition, and inflammatory cell and mast cell infiltration, which involved anti-inflammatory, antioxidant, and anti-apoptotic effects. Further, fRGM suppressed hypertrophic changes and inflammatory responses in the spleen and lymph nodes. The beneficial effects were observed in the dexamethasone and fRGM groups; however, the antioxidant effects were evident only in the fRGM treatments. These results provide useful information for developing fRGM as a therapeutic source for AD.
Variable Rate Independently Recurrent Neural Network (IndRNN) for Action Recognition
Authors: Gao Yanbo , Li Chuankun , Li Shuai , Cai Xun , Ye Mao , Yuan Hui
Published: March 2022

Recurrent neural networks (RNNs) have been widely used to solve sequence problems due to their capability of modeling temporal dependency. Despite the rich varieties of RNN models proposed in the literature, the problem of different sampling rates or performing speeds in sequence tasks has not been explicitly considered in the network and the corresponding training and testing processes. This paper addresses the problem of different sampling rates or performing speeds in the skeleton-based action recognition with RNNs. Specifically, the recently proposed independently recurrent neural network (IndRNN) is used as the RNN network due to its well-behaved and easily regulated gradient backpropagation through time. Samples are extracted with variable sampling rates and thus of different lengths, then processed by IndRNN with different time steps. In order to accommodate the differences in terms of gradients introduced by the backpropagation through time under variable time steps, a learning rate adjustment method is further proposed in the paper. Different learning rate adjustment factors are obtained for different layers by analyzing the gradient behavior under IndRNN. Experiments on skeleton-based action recognition are conducted to verify its effectiveness, and the results show that the proposed variable rate IndRNN network can significantly improve the performance over the RNN models under the conventional training strategies.
Effects of Indoor Air Pollution on the Development of Children under Five Years of Age in Sri Lanka
Authors: Ranathunga Nayomi , Perera Priyantha , Nandasena Sumal , Sathiakumar Nalini , Kasturiratne Anuradhani , Wickremasinghe Rajitha
Published: March 2022

Air pollution is a multifaceted environmental toxin affecting the Central Nervous System (CNS) through diverse pathways. The CNS of young children is particularly susceptible to the detrimental effects of toxins, as brain development continues postnatally with the formation of interneuronal connections, glial cell proliferation and myelination of axons. Indoor air pollution (IAP) from solid fuel combustion is more harmful than outdoor air pollution. Numerous air pollutants hazardous to health are released during the burning of unprocessed biomass. The primary source of fuel in Sri Lanka for cooking is biomass, mainly wood. In this study, we evaluated the influence of IAP resulting from biomass combustion on the neurodevelopment of children. In a cohort of children under five years living in a semi-urban area of Sri Lanka, neurodevelopment was assessed using Denver II developmental screening test. Air quality levels were measured (Carbon Monoxide (CO) and Particulate Matter 2.5 (PM2.5)) in a subsample. There were significantly high levels of CO and PM2.5 in the ambient air of households using biomass as the primary fuel for cooking. Children living in these households had a significantly higher number of children with ‘suspect’ developmental assessment scores in the language, social behavior and play and gross motor development domains.
Contrary Responses of the Gulf Stream and the Kuroshio to Arctic Sea Ice Loss
Authors: Wang Kun , Wu Linyue , Liu Haiwen , Dan Bo , Dai Haijin , Deser Clara
Published: March 2022

The impact on the Gulf Stream and Kuroshio from Arctic sea ice loss is investigated using the Community Climate System Model version 4 (CCSM4) model for their important roles during climate change. Results show that the Gulf Stream (Kuroshio) weakens (strengthens) in response to Arctic sea ice loss via ocean (atmosphere) adjustments. More precisely, the Kuroshio acceleration is mainly due to the anomalous wind stress over the North Pacific, while the ocean gyre adjustments in the Atlantic are responsible for the weakened Gulf Stream. As positive buoyancy fluxes induced by Arctic sea ice loss trigger a slowdown of the Atlantic Meridional Overturning Circulation (AMOC), the Gulf Stream decelerates evidently and the current speed decreases by about 5–8 cm/s in the upper ocean. Resulting from less advection and horizontal diffusion in the temperature budget, less poleward warm water leads to narrow sea surface cooling sandwiched between strong warming in the subpolar and subtropical Atlantic. Furthermore, colder surface decreases the upward heat flux (mainly latent heat flux) along the Gulf Stream Extension (GE) path, which leads to a warming hole in the atmosphere.
Carbonic Anhydrase III Has Potential as a Biomarker for Experimental Colitis and Functions as an Immune Regulator by Inhibiting Inflammatory Cytokine Secretion
Authors: Okada Kohki , Ikemoto Masaki
Published: March 2022

Ulcerative colitis (UC) is characterized by chronic inflammation of the large intestine, repeated remissions, and symptom relapses. Although unknown components in colonic regions are deeply involved in the pathogenesis of UC, the causes of UC development and aggravation have not yet been elucidated in detail. To identify key factors, we investigated the changes in protein components in the large intestine of rats with dextran sulfate sodium-induced experimental colitis (UCR). The components that differed in their concentration between normal rats (WT) and UCR were carefully investigated by electrophoretic separation and mass spectrometry. Based on these results, seven proteins with different expression levels between the WT and UCR were observed. Among them, we focused on carbonic anhydrase III (CA-III) in the pathogenesis of UC. CA-III concentrations in the colon tissue and serum were quantitatively measured using an enzyme-linked immunosorbent assay (ELISA) and real-time PCR, and the levels significantly decreased in both the colon tissue and serum of UCR with the aggravation of experimental UC. In an in vitro assay, CA-III function in peritoneal macrophages (MΦ) from rats was investigated. Upon stimulation of MΦ with lipopolysaccharide (LPS), the CA-III concentration significantly decreased in the cytoplasm of these cells. MΦ treated with an anti-CAIII antibody followed by stimulation with LPS actively secreted inflammatory cytokines, particularly interleukin-6 and tumor necrosis factor-α. Therefore, CA-III in MΦ appears to be an immune regulator that suppresses the secretion of inflammatory cytokines.
Cholinergic Regulation of Hippocampal Theta Rhythm
Authors: Gu Zhenglin , Yakel Jerrel L.
Published: March 2022

Cholinergic regulation of hippocampal theta rhythm has been proposed as one of the central mechanisms underlying hippocampal functions including spatial memory encoding. However, cholinergic transmission has been traditionally associated with atropine-sensitive type II hippocampal theta oscillations that occur during alert immobility or in urethane-anesthetized animals. The role of cholinergic regulation of type I theta oscillations in behaving animals is much less clear. Recent studies strongly suggest that both cholinergic muscarinic and nicotinic receptors do actively regulate type I hippocampal theta oscillations and thus provide the cholinergic mechanism for theta-associated hippocampal learning. Septal cholinergic activation can regulate hippocampal circuit and theta expression either through direct septohippocampal cholinergic projections, or through septal glutamatergic and GABAergic neurons, that can precisely entrain hippocampal theta rhythmicity.
Epigenetic Factors as Etiological Agents, Diagnostic Markers, and Therapeutic Targets for Luminal Breast Cancer
Authors: Thang Nguyen Xuan , Yoo Seonho , La Hyeonwoo , Lee Hyeonji , Park Chanhyeok , Park Kyoung Sik , Hong Kwonho
Published: March 2022

Luminal breast cancer, an etiologically heterogeneous disease, is characterized by high steroid hormone receptor activity and aberrant gene expression profiles. Endocrine therapy and chemotherapy are promising therapeutic approaches to mitigate breast cancer proliferation and recurrence. However, the treatment of therapy-resistant breast cancer is a major challenge. Recent studies on breast cancer etiology have revealed the critical roles of epigenetic factors in luminal breast cancer tumorigenesis and drug resistance. Tumorigenic epigenetic factor-induced aberrant chromatin dynamics dysregulate the onset of gene expression and consequently promote tumorigenesis and metastasis. Epigenetic dysregulation, a type of somatic mutation, is a high-risk factor for breast cancer progression and therapy resistance. Therefore, epigenetic modulators alone or in combination with other therapies are potential therapeutic agents for breast cancer. Several clinical trials have analyzed the therapeutic efficacy of potential epi-drugs for breast cancer and reported beneficial clinical outcomes, including inhibition of tumor cell adhesion and invasiveness and mitigation of endocrine therapy resistance. This review focuses on recent findings on the mechanisms of epigenetic factors in the progression of luminal breast cancer. Additionally, recent findings on the potential of epigenetic factors as diagnostic biomarkers and therapeutic targets for breast cancer are discussed.
Effect of Melatonin on Psoriatic Phenotype in Human Reconstructed Skin Model
Authors: Scuderi Sarah Adriana , Cucinotta Laura , Filippone Alessia , Lanza Marika , Campolo Michela , Paterniti Irene , Esposito Emanuela
Published: March 2022

Psoriasis is an inflammatory and auto-immune skin-disease characterized by uncontrolled keratinocyte proliferation. Its pathogenesis is not still fully understood; however, an aberrant and excessive inflammatory and immune response can contribute to its progression. Recently, more attention has been given to the anti-inflammatory and immunomodulators effects of melatonin in inflammatory diseases. The aim of this paper was to investigate the effect of melatonin on psoriatic phenotype and also in S. aureus infection-associated psoriasis, with an in vitro model using Skinethic Reconstructed Human Epidermis (RHE). An in vitro model was constructed using the RHE, a three-dimensional-model obtained from human primary-keratinocytes. RHE-cells were exposed to a mix of pro-inflammatory cytokines, to induce a psoriatic phenotype; cells were also infected with S. aureus to aggravate psoriasis disease, and then were treated with melatonin at the concentrations of 1 nM, 10 nM, and 50 nM. Our results demonstrated that melatonin at higher concentrations significantly reduced histological damage, compared to the cytokine and S. aureus groups. Additionally, the treatment with melatonin restored tight-junction expression and reduced pro-inflammatory cytokine levels, such as interleukin-1β and interleukin-12. Our results suggest that melatonin could be considered a promising strategy for psoriasis-like skin inflammation, as well as complications of psoriasis, such as S. aureus infection.
First Recombinant High-Density Lipoprotein Particles Administration in a Severe ICU COVID-19 Patient, a Multi-Omics Exploratory Investigation
Authors: Tanaka Sébastien , Begue Floran , Veeren Bryan , Tran-Dinh Alexy , Robert Tiphaine , Tashk Parvine , Lortat-Jacob Brice , Faille Dorothée , de Chaisemartin Luc , Zappella Nathalie , Atchade Enora , Kramer Laura , Montravers Philippe , Meilhac Olivier
Published: March 2022

High-density lipoproteins (HDLs) have multiple endothelioprotective properties. During SARS-CoV-2 infection, HDL-cholesterol (HDL-C) concentration is markedly reduced, and studies have described severe impairment of the functionality of HDL particles. Here, we report a multi-omic investigation of the first administration of recombinant HDL (rHDL) particles in a severe COVID-19 patient in an intensive care unit. Plasma ApoA1 increased and HDL-C decreased after each recombinant HDL injection, suggesting that these particles were functional in terms of reverse cholesterol transport. The proportion of large HDL particles also increased after injection of recombinant HDL. Shotgun proteomics performed on HDLs isolated by ultracentrifugation indicated that ApoA1 was more abundant after injections whereas most of the pro-inflammatory proteins identified were less abundant. Assessment of Serum amyloid A-1, inflammatory markers, and cytokines showed a significant decrease for most of them during recombinant HDL infusion. Our results suggest that recombinant HDL infusion is feasible and a potential therapeutic strategy to be explored in COVID-19 patients.
Solar Performance Metrics in Urban Planning: A Review and Taxonomy
Authors: Czachura Agnieszka , Kanters Jouri , Gentile Niko , Wall Maria
Published: March 2022

Metrics are instrumental in design assessments. Solar performance metrics help designers to evaluate solar access in cities. Metrics should be used early in the urban planning stages in order to enable sustainable urban development with greater access to solar energy. Currently, solar assessments at this design stage are limited in practice; established methods or routines are lacking, and so are suitable metrics. This paper reviews the relevant literature to provide a critical overview of solar metrics commonly used in building performance assessments. The review defines key metric formulation principles—valuation, time constraint, and normalisation—which should be considered when designing a performance indicator. A new taxonomy of solar performance metrics is provided. Metric definitions, suitability, and limitations are discussed. The findings highlight the need for reliable, low-complexity metrics and adequate methods for early solar assessments for urban planning.
Role of Adjuvant Radiotherapy in Non-Small Cell Lung Cancer—A Review
Authors: Süveg Krisztian , Plasswilm Ludwig , Iseli Thomas , Leskow Pawel , Fischer Galina Farina , Putora Paul Martin
Published: March 2022

Background: For patients with completely resected non-small cell lung cancer (NSCLC) with ipsilateral mediastinal lymph node involvement (pN2), the administration of adjuvant chemotherapy is the standard of care. The role of postoperative radiation therapy (PORT) is controversial. Methods: We describe the current literature focusing on the role of PORT in completely resected NSCLC patients with pN2 involvement and reflect on its role in current guidelines. Results: Based on the results of the recent Lung ART and PORT-C trials, the authors conclude that PORT cannot be generally recommended for all resected pN2 NSCLC patients. A substantial decrease in the locoregional relapse rate without translating into a survival benefit suggests that some patients with risk factors might benefit from PORT. This must be balanced against the risk of cardiopulmonary toxicity with potentially associated mortality. Lung ART has already changed the decision making for the use of PORT in daily practice for many European lung cancer experts, with lower rates of recommendations for PORT overall. Conclusions: PORT is still used, albeit decreasingly, for completely resected NSCLC with pN2 involvement. High-level evidence for its routine use is lacking. Further analyses are required to identify patients who would potentially benefit from PORT.
RETRACTED: Lysosomes in Stem Cell Quiescence: A Potential Therapeutic Target in Acute Myeloid Leukemia
Authors: Jain Vaibhav , Bose Swaroop , Arya Awadhesh , Arif Tasleem
Published: March 2022

Lysosomes are cellular organelles that regulate essential biological processes such as cellular homeostasis, development, and aging. They are primarily connected to the degradation/recycling of cellular macromolecules and participate in cellular trafficking, nutritional signaling, energy metabolism, and immune regulation. Therefore, lysosomes connect cellular metabolism and signaling pathways. Lysosome’s involvement in the critical biological processes has rekindled clinical interest towards this organelle for treating various diseases, including cancer. Recent research advancements have demonstrated that lysosomes also regulate the maintenance and hemostasis of hematopoietic stem cells (HSCs), which play a critical role in the progression of acute myeloid leukemia (AML) and other types of cancer. Lysosomes regulate both HSCs’ metabolic networks and identity transition. AML is a lethal type of blood cancer with a poor prognosis that is particularly associated with aging. Although the genetic landscape of AML has been extensively described, only a few targeted therapies have been produced, warranting the need for further research. This review summarizes the functions and importance of targeting lysosomes in AML, while highlighting the significance of lysosomes in HSCs maintenance.
High Keratin-7 Expression in Benign Peri-Tumoral Prostatic Glands Is Predictive of Bone Metastasis Onset and Prostate Cancer-Specific Mortality
Authors: Dariane Charles , Clairefond Sylvie , Péant Benjamin , Communal Laudine , Thian Zhe , Ouellet Véronique , Trudel Dominique , Benzerdjeb Nazim , Azzi Feryel , Méjean Arnaud , Timsit Marc-Olivier , Baurès Manon , Guidotti Jacques-Emmanuel , Goffin Vincent , Karakiewicz Pierre I. , Mes-Masson Anne-Marie , Saad Fred
Published: March 2022

Background: New predictive biomarkers are needed to accurately predict metastasis-free survival (MFS) and cancer-specific survival (CSS) in localized prostate cancer (PC). Keratin-7 (KRT7) overexpression has been associated with poor prognosis in several cancers and is described as a novel prostate progenitor marker in the mouse prostate. Methods: KRT7 expression was evaluated in prostatic cell lines and in human tissue by immunohistochemistry (IHC, on advanced PC, n = 91) and immunofluorescence (IF, on localized PC, n = 285). The KRT7 mean fluorescence intensity (MFI) was quantified in different compartments by digital analysis and correlated to clinical endpoints in the localized PC cohort. Results: KRT7 is expressed in prostatic cell lines and found in the basal and supra-basal compartment from healthy prostatic glands and benign peri-tumoral glands from localized PC. The KRT7 staining is lost in luminal cells from localized tumors and found as an aberrant sporadic staining (2.2%) in advanced PC. In the localized PC cohort, high KRT7 MFI above the 80th percentile in the basal compartment was significantly and independently correlated with MFS and CSS, and with hypertrophic basal cell phenotype. Conclusion: High KRT7 expression in benign glands is an independent biomarker of MFS and CSS, and its expression is lost in tumoral cells. These results require further validation on larger cohorts.
Symptom Prevalence in Spanish and Colombian Oncology Patients Measured with the MSAS
Authors: Llamas-Ramos Inés , Llamas-Ramos Rocío , Carrillo-González Gloria Mabel , Sepúlveda-Ramírez Jennifer , Vargas-Rosero Elizabeth
Published: March 2022

Cancer patients experience several symptoms throughout their illness and the treatments they receive. While this pathology represents a worldwide health problem, knowing the symptom prevalence in oncology patients remains a challenge; validated tools to obtain this information are essential. Furthermore, this prevalence information would help health professionals to establish appropriate treatments. The objective of this study was to ascertain the symptom prevalence in cancer patients from Spain and Colombia to improve future approaches. The frequency, severity, and distress of 32 symptoms from a validated scale experienced by cancer patients from Spain and Colombia were measured. Two hundred and forty-six patients (49.7%) who attended the Day University Hospital of Salamanca (Spain) and two hundred and forty-nine outpatients (50.3%) of the San Diego Cancer Center (Colombia) between 2016 and 2019 participated in the study. All patients filled in the Assessment Scale only once. Four hundred and ninety-five patients (333 women (67.3%) and 162 men (32.7%)) completed the Memorial Symptom Assessment Scale (Spanish version). The most frequent symptom for both samples (Spanish and Colombian) was “lack of energy” (38.4% and 13.7%, respectively). The most severe symptoms for the Spanish and Colombian samples were “problems with sexual interest or activity” (38.4%) and “dry mouth” (13.7%), respectively, and both samples agreed on the most distressing symptom: “hair loss” (Spanish, 38%; Colombian, 10.1%). The Spanish version of the MSAS has proven to be a valid and reliable tool in Spanish-speaking countries to obtain the most prevalent, severe, and distressing symptoms in Spanish and Colombian oncology patients. The prevalence of symptoms was demonstrated to be similar across both countries, and the results will help to design and adapt treatments for cancer patients, targeting these symptoms to reduce or avoid them and thus improving their quality of life.