bluuebunny/crossref_metadata_2025 huggingface dataset:
The Overactivation of NADPH Oxidase during Clonorchis sinensis Infection and the Exposure to N-Nitroso Compounds Promote Periductal Fibrosis
Authors: Jeong Ji Hoon , Yi Junyeong , Hwang Myung Ki , Hong Sung-Jong , Sohn Woon-Mok , Kim Tong-Soo , Pak Jhang Ho
Published: May 2021

Clonorchis sinensis, a high-risk pathogenic human liver fluke, provokes various hepatobiliary complications, including epithelial hyperplasia, inflammation, periductal fibrosis, and even cholangiocarcinogenesis via direct contact with worms and their excretory–secretory products (ESPs). These pathological changes are strongly associated with persistent increases in free radical accumulation, leading to oxidative stress-mediated lesions. The present study investigated C. sinensis infection- and/or carcinogen N-nitrosodimethylamine (NDMA)-associated fibrosis in cell culture and animal models. The treatment of human cholangiocytes (H69 cells) with ESPs or/and NDMA increased reactive oxidative species (ROS) generation via the activation of NADPH oxidase (NOX), resulting in augmented expression of fibrosis-related proteins. These increased expressions were markedly attenuated by preincubation with a NOX inhibitor (diphenyleneiodonium chloride) or an antioxidant (N-acetylcysteine), indicating the involvement of excessive NOX-dependent ROS formation in periductal fibrosis. The immunoreactive NOX subunits, p47phox and p67phox, were observed in the livers of mice infected with C. sinensis and both infection plus NDMA, concomitant with collagen deposition and immunoreactive fibronectin elevation. Staining intensities are proportional to lesion severity and infection duration or/and NDMA administration. Thus, excessive ROS formation via NOX overactivation is a detrimental factor for fibrogenesis during liver fluke infection and exposure to N-nitroso compounds.
Applications of Smart Glasses in Applied Sciences: A Systematic Review
Authors: Kim Dawon , Choi Yosoon
Published: May 2021

The aim of this study is to review academic papers on the applications of smart glasses. Among 82 surveyed papers, 57 were selected through filtering. The papers were published from January 2014 to October 2020. Four research questions were set up using the systematic review method, and conclusions were drawn focusing on the research trends by year and application fields; product and operating system; sensors depending on the application purpose; and data visualization, processing, and transfer methods. It was found that the most popular commercial smart glass products are Android-based Google products. In addition, smart glasses are most often used in the healthcare field, particularly for clinical and surgical assistance or for assisting mentally or physically disabled persons. For visual data transfer, 90% of the studies conducted used a camera sensor. Smart glasses have mainly been used to visualize data based on augmented reality, in contrast with the use of mixed reality. The results of this review indicate that research related to smart glasses is steadily increasing, and technological research into the development of smart glasses is being actively conducted.
Anterior Cruciate Ligament Injury Prevention Exercises: Could a Neuromuscular Warm-Up Improve Muscle Pre-Activation before a Soccer Game? A Proof-of-Principle Study on Professional Football Players
Authors: de Sire Alessandro , Demeco Andrea , Marotta Nicola , Moggio Lucrezia , Palumbo Arrigo , Iona Teresa , Ammendolia Antonio
Published: May 2021

Neuromuscular warm-up has been shown to decrease the risk of anterior cruciate ligament (ACL) injury improving muscular firing patterns. All preventive training programs described in the literature have a duration of several weeks. To date, no studies have explored the immediate effect of a neuromuscular warm-up exercise on pre-activation time of the knee stabilizer muscles. Thus, this proof-of-principle study aimed at evaluating the acute effects of a neuromuscular warm-up exercises on the electromyographic activation of knee stabilizer muscles’ activation pattern. We included 11 professional football players, mean aged 23.2 ± 4.5 years, from a Southern Italy football team. All of them underwent a standard warm-up exercise protocol at the first day of the evaluation. At 1 week, they underwent a structured neuromuscular warm-up exercise protocol. We assessed as outcome measure the pre-activation time (ms) of rectus femoris (RF), vastus medialis (VM), biceps femoris (BF), and medial hamstrings (MH) upon landing. Outcomes were assessed before and after the standard warm-up and neuromuscular warm-up. Pre-activation time of RF, VM, BF and MH significantly improved only after neuromuscular warm-up (p < 0.05); moreover, there was a significant (p < 0.05) between-group difference in pre-activation time of all muscles after the neuromuscular warm-up compared with the standard warm-up. These findings suggested that physical exercise consisting of a structured injury prevention neuromuscular warm-up might have an immediate effect in improving the activation time of the knee stabilizer muscles, thus potentially reducing the risk of ACL injury.
Sustainability Levels in Comparison with Mechanical Properties and Durability of Pumice High-Performance Concrete
Authors: Hrabová Kristýna , Lehner Petr , Ghosh Pratanu , Konečný Petr , Teplý Břetislav
Published: May 2021

In the production of cement and concrete, mechanical and durable properties are essential, along with reasonable cost and sustainability. This study aimed to apply an evaluation procedure of the level of sustainability of mixtures of high-performance concretes (HPC) with various eco-friendly supplementary cementitious materials (SCM). The major supplementary cementitious materials (SCMs), namely, volcanic pumice pozzolan (VPP), Class C and F fly ash, ground granulated blast furnace slag of grade 120, silica fume, and metakaolin, were included. Twenty-seven concrete mixtures were analyzed using a previously presented comprehensive material sustainability indicator in a cost-effective variant. The results indicated that the rank of the concretes differed at 28, 56, and 91 days after concreting. In addition, the study showed no correlation of strength and diffusion parameters with sustainability indicators. Finally, this study will contribute to the optimal selection of mixtures of HPC with VPP in terms of sustainability, cost, and durability for future implementation in reinforced concrete bridge deck slabs and pavements. The values of sustainability indicators for pumice-based mixtures were compared with those for other SCMs, highlighting the sustainable performance of volcanic ash-based SCM.
Comparing Methods of DC Motor Control for UUVs
Authors: Shah Rohan , Sands Timothy
Published: May 2021

Adaptive and learning methods are proposed and compared to control DC motors actuating control surfaces of unmanned underwater vehicles. One type of adaption method referred to as model-following is based on algebraic design, and it is analyzed in conjunction with parameter estimation methods such as recursive least squares, extended least squares, and batch least squares. Another approach referred to as deterministic artificial intelligence uses the process dynamics defined by physics to control output to track a necessarily specified autonomous trajectory (sinusoidal versions implemented here). In addition, one instantiation of deterministic artificial intelligence uses 2-norm optimal feedback learning of parameters to modify the control signal, while another instantiation is presented with proportional plus derivative adaption. Model-following and deterministic artificial intelligence are simulated, and respective performance metrics for transient response and input tracking are evaluated and compared. Deterministic artificial intelligence outperformed the model-following approach in minimal peak transient value by a percent range of approximately 2–70%, but model-following achieved at least 29% less error in input tracking than deterministic artificial intelligence. This result is surprising and not in accordance with the recently published literature, and the explanation of the difference is theorized to be efficacy with discretized implementations.
Therapeutic Advances in Tendinopathy Quantified Microscopically Using Bonar Score, with a Special Reference to PRP Therapy—A Systematic Review of Experimental Studies
Authors: Zabrzyński Jan , Gagat Maciej , Huri Gazi , Łapaj Łukasz , Paczesny Łukasz , Zielińska Wioletta , Zabrzyńska Maria , Szwedowski Dawid , Kruczyński Jacek
Published: May 2021

(1) Background: The Bonar scoring system serves in the microscopic evaluation of tendon pathology. However, it can be easily adapted to investigate decreasing degeneration after treatment and quantify the healing progress. We believe that there is an actual need for a connection between clinical observations and tissue alterations arising during the treatment process, to gain superior functional outcomes. Herein, we perform a systematic review of the Bonar score’s application in the histopathological assessment of therapeutic advances in tendinopathy, with special reference to PRP therapy. (2) Methods: A systematic review was performed based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The searching strategy was conducted across major databases: PubMed, Cochrane Central, ScienceDirect, SciELO, and Web of Science. The risk-of-bias assessment was made using the Cochrane Collaboration’s Risk of Bias Tool and SYRCLE. (3) Results: The searching strategy produced 807 articles, and after selection, 22 studies were included. We collected 21 animal studies (n = 472) and 1 human study (n = 45). Three types of tendons were taken into account: 14 Achilles tendons, 7 supraspinatus tendons (SST), and in 1 case, Achilles and patellar tendons simultaneously. A variety of therapeutic methods were used—from intra-tendinous substance injections to surgical procedures or mechanical stimuli—but platelet-rich plasma (PRP) therapy dominated among them and was present in six studies. Most of the collected studies included an assessment of the tendons’ histopathology based on the classical Bonar score (with four variables and one observer). The staining protocol was based on the hematoxylin and eosin technique. An evaluation of therapeutic effects showed 15 positive results, 6 negative results, and 1 neutral result of treatments. (4) Conclusions: To understand the tendinopathy phenomenon, a link between histopathology and clinical observations in chronic tendon disorders is required due to the possibility of functional outcome improvements. The Bonar scoring system is well established in tendon pathology assessment and could also be adopted to assess therapeutic results in tendon disorders. Studies that included the PRP application showed Bonar-scoring-system-based evidence of superior tendinous tissue healing related to improved clinical results.
Using CO2 as a Cooling Fluid for Power Plants: A Novel Approach for CO2 Storage and Utilization
Authors: Phuoc Tran X. , Massoudi Mehrdad
Published: May 2021

To our knowledge, the potential use of CO2 as a heat-transmitting fluid for cooling applications in power plants has not been explored very extensively. In this paper, we conduct a theoretical analysis to explore the use of CO2 as the heat transmission fluid. We evaluate and compare the thermophysical properties of both dry air and CO2 and perform a simple analysis on a steam-condensing device where steam flows through one of the flow paths and the cooling fluid (CO2 or air) is expanded from a high-pressure container and flows through the other. Sample calculations are carried out for a saturated-vapor steam at 0.008 MPa and 41.5 °C with the mass flow rate of 0.01 kg/s. The pressure of the storage container ranges from 1 to 5 MPa, and its temperature is kept at 35 °C. The pressure of the cooling fluid (CO2 or dry air) is set at 0.1 MPa. With air as the heat-removing fluid, the steam exits the condensing device as a vapor-liquid steam of 53% to 10% vapor for the container pressure of 1 to 5 MPa. With CO2 as the heat-removing fluid, the steam exits the device still containing 44% and 7% vapor for the container pressure of 1 MPa and 2 MPa, respectively. For the container pressure of 3 MPa and higher, the steam exits the device as a single-phase saturated liquid. Thus, due to its excellent Joule–Thomson cooling effect and heat capacity, CO2 is a better fluid for power plant cooling applications. The condensing surface area is also estimated, and the results show that when CO2 is used, the condensing surface is 50% to 60% less than that when dry air is used. This leads to significant reductions in the condenser size and the capital costs. A rough estimate of the amount of CO2 that can be stored and utilized is also carried out for a steam power plant which operates with steam with a temperature of 540 °C (813 K) and a pressure of 10 MPa at the turbine inlet and saturated-vapor steam at 0.008 MPa at the turbine outlet. The results indicate that if CO2 is used as a cooling fluid, CO2 emitted from a 1000 MW power plant during a period of 250 days could be stored and utilized.
Coordinated Operation of Electricity and Natural Gas Networks with Consideration of Congestion and Demand Response
Authors: Lai Chun Sing , Yan Mengxuan , Li Xuecong , Lai Loi Lei , Xu Yang
Published: May 2021

This work presents a new coordinated operation (CO) framework for electricity and natural gas networks, considering network congestions and demand response. Credit rank (CR) indicator of coupling units is introduced, and gas consumption constraints information of natural gas fired units (NGFUs) is given. Natural gas network operator (GNO) will deliver this information to an electricity network operator (ENO). A major advantage of this operation framework is that no frequent information interaction between GNO and ENO is needed. The entire framework contains two participants and three optimization problems, namely, GNO optimization sub-problem-A, GNO optimization sub-problem-B, and ENO optimization sub-problem. Decision sequence changed from traditional ENO-GNO-ENO to GNO-ENO-GNO in this novel framework. Second-order cone (SOC) relaxation is applied to ENO optimization sub-problem. The original problem is reformulated as a mixed-integer second-order cone programming (MISOCP) problem. For GNO optimization sub-problem, an improved sequential cone programming (SCP) method is applied based on SOC relaxation and the original sub-problem is converted to MISOCP problem. A benchmark 6-node natural gas system and 6-bus electricity system is used to illustrate the effectiveness of the proposed framework. Considering pipeline congestion, CO, with demand response, can reduce the total cost of an electricity network by 1.19%, as compared to −0.48% using traditional decentralized operation with demand response.
Numerical Simulation of Intrachamber Processes in the Power Plant
Authors: Benderskiy Boris , Frankovský Peter , Chernova Alena
Published: May 2021

This paper considers the issues of numerical modeling of nonstationary spatial gas dynamics in the pre-nozzle volume of the combustion chamber of a power plant with a cylindrical slot channel at the power plant of the mass supply surface. The numerical simulation for spatial objects is based on the solution conjugate problem of heat exchange by the control volume method in the open integrated platform for numerical simulation of continuum mechanics problems (openFoam). The calculation results for gas-dynamic and thermal processes in the power plant with a four-nozzle cover are presented. The analysis of gas-dynamic parameters and thermal flows near the nozzle cover, depending on the canal geometry, is given. The topological features of the flow structure and thermophysical parameters near the nozzle cap were studied. For the first time, the transformation of topological features of the flow structure in the pre-nozzle volume at changes in the mass channel’s geometry is revealed, described, and analyzed. The dependence of the Nusselt number in the central point of stagnation on the time of the power plants operation is revealed.
Study on Rock Damage Mechanism for Lateral Blasting under High In Situ Stresses
Authors: Huo Xiaofeng , Shi Xiuzhi , Qiu Xianyang , Chen Hui , Zhou Jian , Zhang Shian , Rao Dijun
Published: May 2021

A 3D numerical model was presented to investigate the blast-induced damage characteristics of highly stressed rock mass. The RHT (Riedel, Hiermaier, and Thoma) model in LS-DYNA was used to simulate the blast-induced damage and its parameters were calibrated by a physical model test. Based on the calibrated numerical model, the influences of confining pressure and free surface span on the blast-induced damage characteristics were investigated. The results show that under uniaxial loading, the crater volume increases with confining pressure increases. The uniaxial static load can change the optimal burden and the critical embedding depth of charge. In stressed rock, the variation law of the crater shape affected by radial tensile fractures is opposite to that affected by reflected tensile fractures. Under the biaxial static load, the crater volume of the borehole placed on the side of the max static load is greater than the other side. The explosion crater can be improved by increasing the free surface span on the same side. Finally, it is suggested that the blasting efficiency can be improved by preferentially detonating the charge on the side of the max static load, and then the charge on the other side can be detonated with a wider free surface span.
Trace CH4 Gas Detection Based on an Integrated Spherical Photoacoustic Cell
Authors: Jiao Yexiang , Fan Hongji , Gong Zhenfeng , Yang Kai , Shen Feiyang , Chen Ke , Mei Liang , Peng Wei , Yu Qingxu
Published: May 2021

This paper presents an integrated spherical photoacoustic cell (SPAC) for trace methane (CH4) gas detection. Theoretical analysis and analogue simulations are carried out to analyze the acoustic field distribution of the SPAC at resonant and non-resonant modes. The finite element simulation results based on COMSOL show that the first-order radial resonant frequency and second-order angular resonant frequency are 24,540 Hz and 18,250 Hz, respectively, which show good agreements with the formula analysis results. The integrated SPAC, together with a high-speed spectrometer and a distributed feedback (DFB) laser source, makes up a photoacoustic (PA) spectroscopy (PAS) system, which is employed for CH4 detection. The minimum detection limit (MDL) is measured to be 126.9 parts per billion (ppb) at an average time of 1000 s. The proposed SPAC has an integrated, miniaturized and all-optical structure, which can be used for remote and long-distance trace gas detection.
Seismic Design and Performance Assessment of Frame Buildings Reinforced by Dual-Phase Steel
Authors: Žižmond Jure , Dolšek Matjaž
Published: May 2021

To improve the durability and serviceability of reinforced concrete structures, different variants of dual-phase reinforcing steel were developed within the research project NEWREBAR. The investigated variant of the new material, termed DPD2 steel, has a specific microstructure that increases the corrosion resistance, but its yielding strength is less than that of Tempcore steel B500B. DPD2 steel has no yielding plateau, which is characteristic of conventional reinforcing steel. Thus, it was investigated whether the current building codes can be used to design earthquake-resistant concrete structures reinforced by DPD2 steel bars. For this reason, three multi-story reinforced concrete frame buildings were designed according to Eurocode by considering DPD2 steel and, for comparison reasons, Tempcore steel B500B. Based on the nonlinear model, which was validated by cyclic test of columns, the seismic performance of DPD2 buildings was found to be improved compared to those designed with conventional B500B reinforcing steel. This can mainly be attributed to the substantial strain hardening of the DPD2 steel, which increases the overstrength factor of the structure by about 10%. However, for the improved seismic performance, the amount of steel in DPD2 buildings had to be increased in the design by approximately 20–25% due to the smaller yield strength of DPD2 steel. Nevertheless, it was demonstrated that Eurocode 8 could be used to design earthquake-resistant frame building reinforced with dual-phase reinforcing steel DPD2.
A Reaction–Diffusion–Reaction System for Forming Periodic Precipitation Bands of Cu-Fe-Based Prussian Blue Analogues
Authors: Hayashi Hisashi , Suzuki Tomoko
Published: May 2021

We propose a simple and novel system to form precipitation patterns of Cu-Fe-based Prussian blue analogues (Cu-Fe PBA) in agarose gel through coupled electrochemical reactions, reactant ion diffusion influenced by electric field, and precipitation reactions. The spatiotemporal evolution, spatial distribution, and crystallite morphologies of the precipitates were investigated by visual inspection, Fe Kα intensity distribution measurements, and optical and scanning electron microscope observations. The observed precipitation patterns and their evolution depended on the applied voltage. Multicolored periodic precipitation bands were stochastically formed under cyclic alternating voltage (4 V for 1 h and then 1 V for 4 h per cycle). The distances between adjacent bands were randomly distributed (0.30 ± 0.25 mm). The sizes and shapes of the crystallites generated in the gel were position-dependent. Cubic but fairly irregular crystallites (0.1–0.8 μm) were formed in the periodic bands, whereas definitely cube-shaped crystallites (1–3 μm) appeared close to the anode. These cube-like reddish–brown crystallites were assigned to Cu-FeII PBA. In some periodic bands, plate-like blue crystallites (assigned to Cu(OH)2) were also present. Future issues for potential applications of the observed periodic banding for selective preparation of Cu-Fe PBA crystallites were discussed.
Development and Preliminary Evaluation of a Lower Body Exosuit to Support Ankle Dorsiflexion
Authors: Schubert Tim , Wollesen Bettina , Weidner Robert
Published: May 2021

For patients suffering from drop foot due to weakness of ankle dorsiflexion muscles, an ankle foot orthosis provides increased foot clearance during the swing phase of gait, but often restricts other gait functions, such as plantarflexion. Due to steady progress in the development of lighter and smaller actuator technologies, active wearable devices such as exosuits become relevant for rehabilitation, since they can offer an extended functionality including a more comfortable wear than passive plastic orthosis. The aim of the paper is to present a lightweight exosuit supporting dorsiflexion during gait with autonomous recognition of gait phases and conditions. One main requirement during the iterative development of the exosuit is a non-restrictive function, thus no differences between the assisted and non-assisted gait of a healthy subject should occur. We therefore conducted a pilot biomechanics study using statistical parametric mapping to analyze kinematics of the ankle joint and muscle activity of m. tibialis anterior of nine subjects without any gait anomalies walking with and without the exosuit. The results show no significant difference between with and without support. In contrast to passive orthosis, the developed system could be an enhanced solution to assist patients suffering from drop foot, which should be analyzed in the next step for evaluating the development.
Environmental Odour Nuisance Assessment in Urbanized Area: Analysis and Comparison of Different and Integrated Approaches
Authors: Zarra Tiziano , Belgiorno Vincenzo , Naddeo Vincenzo
Published: May 2021

Prolonged exposure to odour emissions causes annoyance which leads to nuisance and consequently to complaints. Different methodologies exist in the literature to evaluate odour impacts, but not all are suitable to assess environmental odour nuisance. Information about their applicability criteria and comparison, is scarce and referred to short time analysis. The research presents and discusses the application of different methods to characterize and assess odour nuisance around an industrial plant localized in a sensitive area. Experimental activities are carried out through a long-time analysis programme. Field inspections and predictive methods are investigated and compared. A modification of the traditional dispersion modelling approach is proposed in order to adapt its application for the prediction of the odour nuisance. The offensiveness and location factors are identified as key parameters in the quantification of the perceived nuisance. The integrated dispersion modelling multi-level approach is highlighted as the most suitable for defining the plant strategies. The paper provides useful information to characterize environmental odour problems and identify appropriate solutions for an effective management of odorous sources, with the aim of reducing complaints, restoring the proper relationship between odorous plants and the surrounding communities and increasing the overall quality of the environment.
Adjusting CCIR Maps to Improve Local Behaviour of Ionospheric Models
Authors: Haralambous Haris , Leontiou Theodoros , Petrou Vasilis , Kumar Singh Arun , Charalambides Marios , Lithoxopoulos Nikos , Agisilaou Agis
Published: May 2021

The objective of this article is to present a concept for single-frequency Global Navigation Satellite System (GNSS) positioning local ionospheric mitigation over a certain area. This concept is based on input parameters driving the NeQuick-G algorithm (the ionospheric single-frequency GNSS correction algorithm adopted by Galileo GNSS system), estimated on a local as opposed to a global scale, from ionospheric characteristics measured by a digital ionosonde and a collocated dual-frequency Total Electron Content (TEC) monitor. This approach facilitates the local adjustment of Committee Consultative for Ionospheric Radiowave propagation (CCIR) files and the Az ionization level, which control the ionospheric electron density profile in NeQuick-G, therefore enabling better estimation of positioning errors under quiet geomagnetic conditions. This novel concept for local ionospheric positioning error mitigation may be adopted at any location where ionospheric characteristics foF2 and M(3000)F2 can be measured, as a means to enhance the accuracy of single-frequency positioning applications based on the NeQuick-G algorithm.
Major Floods and Tropical Cyclones over Bangladesh: Clustering from ENSO Timescales
Authors: Wahiduzzaman Md
Published: May 2021

The present study analyzed major floods and tropical cyclones (TCs) over Bangladesh on El Niño-Southern Oscillation (ENSO) timescales. The geographical location, low and almost flat topography have introduced Bangladesh as one of the most vulnerable countries of the world. Bangladesh is highly vulnerable to the extreme hazard events like floods and cyclones which are impacted by ENSO. ENSO is mainly a tropical event, but its impact is global. El Niño (La Niña) represents the warm (cold) phase of the ENSO cycle. Rainfall and cyclonic disturbances data have been used for the period of 70 years (1948–2017) and compared with the corresponding observations of the Southern Oscillation Index. Result shows that major flood events occurred during the monsoon period, and most of them are during the La Niña condition, consistent with the historical archives of flood events in Bangladesh. Synoptic conditions of these events are well matched during La Niña condition. On the other hand, the major TC cases are in the period of either pre-monsoon or post-monsoon season. The pre-monsoon cases are under neutral (developing La Niña) or El Niño and the post-monsoon cases are under La Niña, consistent with climatology studies that La Niña is favorable to have more intense TCs over the Bay of Bengal.
Development and Optimization of Supercritical Fluid Extraction Setup Leading to Quantification of 11 Cannabinoids Derived from Medicinal Cannabis
Authors: Qamar Sadia , Manrique Yady J. , Parekh Harendra S. , Falconer James R.
Published: May 2021

In this study, the optimal setup of supercritical fluid extraction (SFE) was designed and developed, leading to the quantitation of 11 distinct cannabinoids (cannabidivann (CBDV), tetrahydrocannabivann (THCV), cannabidiol (CBD), cannabigerol (CBG) cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabinol (CBN), delta 9-tetrahydrocannabinol (Δ9-THC), delta 8-tetrahydrocannabinol (Δ8-THC), cannabichomere (CBC) and delta 9-tetrahydrocannabinol acid (THCA-A)) extracted from the flowers of medicinal cannabis (sp. Sativa). Supercritical carbon dioxide (scCO2) extraction was performed at 37 °C, a pressure of 250 bar with the maximum theoretical density of CO2 (893.7 kg/m3), which generated the highest yield of cannabinoids from the flower-derived extract. Additionally, a cold separator (separating chamber) was used and positioned immediately after the sample containing chamber to maximize the yield. It was also found that successive washing of the extract with fresh scCO2 further increased yields. Ultra-high performance liquid chromatography coupled with DAD (uHPLC-DAD) was used to develop a method for the quantification of 11 cannabinoids. The C18 stationary phase was used in conjunction with a two solvent system gradient program resulting in the acquisition of the well-resolved chromatogram over a timespan of 32 min. The accuracy and precision of isolated cannabinoids across inter-and intra-day periods were within acceptable limits (<±15%). The assay was also fully validated and deemed sensitive from linearity, LOQ, and LOD perspective. The findings of this body of work are expected to facilitate improved conditions for the optimal extraction of select cannabinoids using scCO2, which holds promise in the development of well-characterized medicinal cannabis formulations. As to our best knowledge, this is the first study to report the uHPLC quantification method for the analysis of 11 cannabinoids from scCO2 extract in a single run with more than 1 min peak separation.
Evaluating the Performance of a Magnetic Nanoparticle-Based Detection Method Using Circle-to-Circle Amplification
Authors: Sánchez Martín Darío , Oropesa-Nuñez Reinier , Zardán Gómez de la Torre Teresa
Published: May 2021

This work explores several issues of importance for the development of a diagnostic method based on circle-to-circle amplification (C2CA) and oligonucleotide-functionalized magnetic nanoparticles. Firstly, the performance of the detection method was evaluated in terms of sensitivity and speed. Synthetic target sequences for Newcastle disease virus and Salmonella were used as model sequences. The sensitivity of the C2CA assay resulted in detection of 1 amol of starting DNA target with a total amplification time of 40 min for both target sequences. Secondly, the functionalization of the nanoparticles was evaluated in terms of robustness and stability. The functionalization was shown to be very robust, and the stability test showed that 92% of the oligos were still attached on the particle surface after three months of storage at 4 °C. Altogether, the results obtained in this study provide a strong foundation for the development of a quick and sensitive diagnostic assay.
EEG Correlates of Old/New Discrimination Performance Involving Abstract Figures and Non-Words
Authors: Toth Monika , Sambeth Anke , Blokland Arjan
Published: May 2021

The processing of pre-experimentally unfamiliar stimuli such as abstract figures and non-words is poorly understood. Here, we considered the role of memory strength in the discrimination process of such stimuli using a three-phase old/new recognition memory paradigm. Memory strength was manipulated as a function of the levels of processing (deep vs. shallow) and repetition. Behavioral results were matched to brain responses using EEG. We found that correct identification of the new abstract figures and non-words was superior to old item recognition when they were merely studied without repetition, but not when they were semantically processed or drawn. EEG results indicated that successful new item identification was marked by a combination of the absence of familiarity (N400) and recollection (P600) for the studied figures. For both the abstract figures and the non-words, the parietal P600 was found to differentiate between the old and new items (late old/new effects). The present study extends current knowledge on the processing of pre-experimentally unfamiliar figurative and verbal stimuli by showing that their discrimination depends on experimentally induced memory strength and that the underlying brain processes differ. Nevertheless, the P600, similar to pre-experimentally familiar figures and words, likely reflects improved recognition memory of meaningless pictorial and verbal items.
Decoding LncRNAs
Authors: Borkiewicz Lidia , Kalafut Joanna , Dudziak Karolina , Przybyszewska-Podstawka Alicja , Telejko Ilona
Published: May 2021

Non-coding RNAs (ncRNAs) have been considered as unimportant additions to the transcriptome. Yet, in light of numerous studies, it has become clear that ncRNAs play important roles in development, health and disease. Long-ignored, long non-coding RNAs (lncRNAs), ncRNAs made of more than 200 nucleotides have gained attention due to their involvement as drivers or suppressors of a myriad of tumours. The detailed understanding of some of their functions, structures and interactomes has been the result of interdisciplinary efforts, as in many cases, new methods need to be created or adapted to characterise these molecules. Unlike most reviews on lncRNAs, we summarize the achievements on lncRNA studies by taking into consideration the approaches for identification of lncRNA functions, interactomes, and structural arrangements. We also provide information about the recent data on the involvement of lncRNAs in diseases and present applications of these molecules, especially in medicine.
Suppression of Breast Cancer by Small Molecules That Block the Prolactin Receptor
Authors: Borcherding Dana C. , Hugo Eric R. , Fox Sejal R. , Jacobson Eric M. , Hunt Brian G. , Merino Edward J. , Ben-Jonathan Nira
Published: May 2021

Prolactin (PRL) is a protein hormone which in humans is secreted by pituitary lactotrophs as well as by many normal and malignant non-pituitary sites. Many lines of evidence demonstrate that both circulating and locally produced PRL increase breast cancer (BC) growth and metastases and confer chemoresistance. Our objective was to identify and then characterize small molecules that block the tumorigenic actions of PRL in BC. We employed three cell-based assays in high throughput screening (HTS) of 51,000 small molecules and identified two small molecule inhibitors (SMIs), named SMI-1 and SMI-6. Both compounds bound to the extracellular domain (ECD) of the PRL receptor (PRLR) at 1–3 micromolar affinity and abrogated PRL-induced breast cancer cell (BCC) invasion and malignant lymphocyte proliferation. SMI-6 effectively reduced the viability of multiple BCC types, had much lower activity against various non-malignant cells, displayed high selectivity, and showed no apparent in vitro or in vivo toxicity. In athymic nude mice, SMI-6 rapidly and dramatically suppressed the growth of PRL-expressing BC xenografts. This report represents a pre-clinical phase of developing novel anti-cancer agents with the potential to become effective therapeutics in breast cancer patients.
Outcomes and Prediction Models for Exclusive Prostate Bed Salvage Radiotherapy among Patients with Biochemical Recurrence after Radical Prostatectomy
Authors: Tseng Chi-Shin , Wang Yu-Jen , Chen Chung-Hsin , Wang Shuo-Meng , Huang Kuo-How , Chow Po-Ming , Pu Yeong-Shiau , Huang Chao-Yuan , Cheng Jason Chia-Hsien
Published: May 2021

Background: The addition of androgen-deprivation therapy (ADT) or pelvic radiation to prostate bed salvage radiotherapy (SRT) has been debated for prostate cancer patients with biochemical recurrence (BCR) after radical prostatectomy. This study aimed to assess the outcomes and propose prediction models for exclusive prostate bed SRT. Methods: This is a prospective observational cohort study with patients who underwent SRT with a pre-SRT PSA < 1.5 ng/mL after radical prostatectomy. Patients were treated with 70-Gy SRT to the prostate bed exclusively. Kaplan–Meier survival analyses and Cox regression analyses were applied for depicting and predicting BCR-free survival, ADT-free survival, and metastasis-free survival (MFS). Regression-based coefficients were used to develop nomograms. Results: A total of 105 patients were included and 91 patients were eligible. The median follow-up period was 39 months. The 5-year BCR-free survival, ADT-free survival, and MFS were 37%, 50%, and 66%, respectively. Multivariable analysis showed that a pre-SRT PSA < 0.45 ng/mL was the only independent factor associated with longer BCR-free survival (p = 0.034), while a PSA-DT > 8 months had better ADT-free survival (p = 0.008). Patients with a PSA-DT > 8 months showed a 100% MFS and a 43% 5-year absolute benefit in MFS than a PSA-DT ≤ 8 months. All patients with a pre-SRT PSA < 0.45 ng/mL and PSA-DT > 8 months were free from subsequent ADT and any metastasis. Conclusions: In patients with a PSA < 0.45 ng/mL and PSA-DT > 8 months for post-prostatectomy BCR, prostate bed SRT provided excellent outcomes without the need for concomitant ADT or pelvic radiotherapy.
Nanoscale Assembly of BiVO4/CdS/CoOx Core–Shell Heterojunction for Enhanced Photoelectrochemical Water Splitting
Authors: Kmentova Hana , Henrotte Olivier , Yalavarthi Rambabu , Haensch Mareike , Heinemann Christian , Zbořil Radek , Schmuki Patrik , Kment Štěpán , Naldoni Alberto
Published: May 2021

Porous BiVO4 electrodes were conformally decorated with CdS via a chemical bath deposition process. The highest photocurrent at 1.1 V vs. RHE was achieved for a BiVO4/CdS composite (4.54 mA cm−2), compared with CdS (1.19 mA cm−2) and bare BiVO4 (2.1 mA cm−2), under AM 1.5G illumination. This improvement in the photoefficiency can be ascribed to both the enhanced optical absorption properties and the charge separation due to the heterojunction formation between BiVO4 and CdS. Furthermore, the BiVO4/CdS photoanode was protected with a CoOx layer to substantially increase the photostability of the material. The new BiVO4/CdS/CoOx nanostructure exhibited a highly stable photocurrent density of ~5 mA cm−2. The capability to produce O2 was locally investigated by scanning photoelectrochemical microscope, which showed a good agreement between photocurrent and O2 reduction current maps. This work develops an efficient route to improve the photo-electrochemical performance of BiVO4 and its long-term stability.
Synthesis and Application of Zeolite Catalysts
Authors: Na Kyungsu
Published: May 2021

Zeolites play a central role in many industrial and chemical engineering processes involving solid catalysts, which have attracted a great deal of attention from chemists, chemical engineers, and materials scientists due to their fascinating features [...]
Ischemia–Reperfusion Injury in Lung Transplantation
Authors: Chen-Yoshikawa Toyofumi Fengshi
Published: May 2021

Lung transplantation has been established worldwide as the last treatment for end-stage respiratory failure. However, ischemia–reperfusion injury (IRI) inevitably occurs after lung transplantation. The most severe form of IRI leads to primary graft failure, which is an important cause of morbidity and mortality after lung transplantation. IRI may also induce rejection, which is the main cause of mortality in recipients. Despite advances in donor management and graft preservation, most donor grafts are still unsuitable for transplantation. Although the pulmonary endothelium is the primary target site of IRI, the pathophysiology of lung IRI remains incompletely understood. It is essential to understand the mechanism of pulmonary IRI to improve the outcomes of lung transplantation. Therefore, we reviewed the state-of-the-art in the management of pulmonary IRI after lung transplantation. Recently, the ex vivo lung perfusion (EVLP) system has been clinically introduced worldwide. Various promising therapeutic strategies for the protection of the endothelium against IRI, including EVLP, inhalation therapy with therapeutic gases and substances, fibrinolytic treatment, and mesenchymal stromal cell therapy, are awaiting clinical application. We herein review the latest advances in the field of pulmonary IRI in lung transplantation.
Challenges with Methods for Detecting and Studying the Transcription Factor Nuclear Factor Kappa B (NF-κB) in the Central Nervous System
Authors: Mostafizar Marina , Cortes-Pérez Claudia , Snow Wanda , Djordjevic Jelena , Adlimoghaddam Aida , Albensi Benedict C.
Published: May 2021

The transcription factor nuclear factor kappa B (NF-κB) is highly expressed in almost all types of cells. NF-κB is involved in many complex biological processes, in particular in immunity. The activation of the NF-κB signaling pathways is also associated with cancer, diabetes, neurological disorders and even memory. Hence, NF-κB is a central factor for understanding not only fundamental biological presence but also pathogenesis, and has been the subject of intense study in these contexts. Under healthy physiological conditions, the NF-κB pathway promotes synapse growth and synaptic plasticity in neurons, while in glia, NF-κB signaling can promote pro-inflammatory responses to injury. In addition, NF-κB promotes the maintenance and maturation of B cells regulating gene expression in a majority of diverse signaling pathways. Given this, the protein plays a predominant role in activating the mammalian immune system, where NF-κB-regulated gene expression targets processes of inflammation and host defense. Thus, an understanding of the methodological issues around its detection for localization, quantification, and mechanistic insights should have a broad interest across the molecular neuroscience community. In this review, we summarize the available methods for the proper detection and analysis of NF-κB among various brain tissues, cell types, and subcellular compartments, using both qualitative and quantitative methods. We also summarize the flexibility and performance of these experimental methods for the detection of the protein, accurate quantification in different samples, and the experimental challenges in this regard, as well as suggestions to overcome common challenges.
Harnessing Astrocytes and Müller Glial Cells in the Retina for Survival and Regeneration of Retinal Ganglion Cells
Authors: Yoo Hyung-Suk , Shanmugalingam Ushananthini , Smith Patrice D.
Published: May 2021

Astrocytes have been associated with the failure of axon regeneration in the central nervous system (CNS), as it undergoes reactive gliosis in response to damages to the CNS and functions as a chemical and physical barrier to axon regeneration. However, beneficial roles of astrocytes have been extensively studied in the spinal cord over the years, and a growing body of evidence now suggests that inducing astrocytes to become more growth-supportive can promote axon regeneration after spinal cord injury (SCI). In retina, astrocytes and Müller cells are known to undergo reactive gliosis after damage to retina and/or optic nerve and are hypothesized to be either detrimental or beneficial to survival and axon regeneration of retinal ganglion cells (RGCs). Whether they can be induced to become more growth-supportive after retinal and optic nerve injury has yet to be determined. In this review, we pinpoint the potential molecular pathways involved in the induction of growth-supportive astrocytes in the spinal cord and suggest that stimulating the activation of these pathways in the retina could represent a new therapeutic approach to promoting survival and axon regeneration of RGCs in retinal degenerative diseases.
The Emerging Role of HDACs: Pathology and Therapeutic Targets in Diabetes Mellitus
Authors: Dewanjee Saikat , Vallamkondu Jayalakshmi , Kalra Rajkumar Singh , Chakraborty Pratik , Gangopadhyay Moumita , Sahu Ranabir , Medala Vijaykrishna , John Albin , Reddy P. Hemachandra , De Feo Vincenzo , Kandimalla Ramesh
Published: May 2021

Diabetes mellitus (DM) is one of the principal manifestations of metabolic syndrome and its prevalence with modern lifestyle is increasing incessantly. Chronic hyperglycemia can induce several vascular complications that were referred to be the major cause of morbidity and mortality in DM. Although several therapeutic targets have been identified and accessed clinically, the imminent risk of DM and its prevalence are still ascending. Substantial pieces of evidence revealed that histone deacetylase (HDAC) isoforms can regulate various molecular activities in DM via epigenetic and post-translational regulation of several transcription factors. To date, 18 HDAC isoforms have been identified in mammals that were categorized into four different classes. Classes I, II, and IV are regarded as classical HDACs, which operate through a Zn-based mechanism. In contrast, class III HDACs or Sirtuins depend on nicotinamide adenine dinucleotide (NAD+) for their molecular activity. Functionally, most of the HDAC isoforms can regulate β cell fate, insulin release, insulin expression and signaling, and glucose metabolism. Moreover, the roles of HDAC members have been implicated in the regulation of oxidative stress, inflammation, apoptosis, fibrosis, and other pathological events, which substantially contribute to diabetes-related vascular dysfunctions. Therefore, HDACs could serve as the potential therapeutic target in DM towards developing novel intervention strategies. This review sheds light on the emerging role of HDACs/isoforms in diabetic pathophysiology and emphasized the scope of their targeting in DM for constituting novel interventional strategies for metabolic disorders/complications.
Innovative Surface Modification Procedures to Achieve Micro/Nano-Graded Ti-Based Biomedical Alloys and Implants
Authors: Li Jie , Zhou Peng , Attarilar Shokouh , Shi Hongyuan
Published: May 2021

Due to the growing aging population of the world, and as a result of the increasing need for dental implants and prostheses, the use of titanium and its alloys as implant materials has spread rapidly. Although titanium and its alloys are considered the best metallic materials for biomedical applications, the need for innovative technologies is necessary due to the sensitivity of medical applications and to eliminate any potentially harmful reactions, enhancing the implant-to-bone integration and preventing infection. In this regard, the implant’s surface as the substrate for any reaction is of crucial importance, and it is accurately addressed in this review paper. For constructing this review paper, an internet search was performed on the web of science with these keywords: surface modification techniques, titanium implant, biomedical applications, surface functionalization, etc. Numerous recent papers about titanium and its alloys were selected and reviewed, except for the section on forthcoming modern implants, in which extended research was performed. This review paper aimed to briefly introduce the necessary surface characteristics for biomedical applications and the numerous surface treatment techniques. Specific emphasis was given to micro/nano-structured topographies, biocompatibility, osteogenesis, and bactericidal effects. Additionally, gradient, multi-scale, and hierarchical surfaces with multifunctional properties were discussed. Finally, special attention was paid to modern implants and forthcoming surface modification strategies such as four-dimensional printing, metamaterials, and metasurfaces. This review paper, including traditional and novel surface modification strategies, will pave the way toward designing the next generation of more efficient implants.
In Memoriam: Yuriy Malyukin
Authors: Zorenko Yuriy
Published: May 2021

On 7 June 2020, Professor Yuriy Malyukin, who was the Guest Editor of a Special Issue of Crystals, titled “Crystals, Films and Nanocomposite Scintillators”, passed away at the age of 63 [...]