bluuebunny/crossref_metadata_2025 huggingface dataset:
N6-methyladenosine and its epitranscriptomic effects on hematopoietic stem cell regulation and leukemogenesis
Authors: Chang Kao-Jung , Shiau Li-Yang , Lin Shiuan-Chen , Cheong Han-Ping , Wang Ching-Yun , Ma Chun , Liang Yan-Wen , Yang Yi-Ping , Ko Po-Shen , Hsu Chih-Hung , Chiou Shih-Hwa
Published: November 2024

N6-methyladenosine (m6A) RNA modification orchestrates cellular epitranscriptome through tuning the homeostasis of transcript stability, translation efficiency, and the transcript affinity toward RNA-binding proteins (RBPs). An aberrant m6A deposition on RNA can lead toward oncogenic expression profile (mRNA), impaired mitochondrial metabolism (mtRNA), and translational suppression (rRNA) of tumor suppressor genes. In addition, non-coding RNAs (ncRNAs), such as X-inactive specific transcript (XIST), miRNAs, and α-ketoglutarate-centric metabolic transcripts are also regulated by the m6A epitranscriptome. Notably, recent studies had uncovered a myriad of m6A-modified transcripts the center of hematopoietic stem cell (HSC) regulation, in which m6A modification act as a context dependent switch to the on and off of hematopoietic stem cell (HSC) maintenance, lineage commitment and terminal differentiation. In this review, we sequentially unfold the m6A mediated epithelial-to-hematopoietic transition in progenitor blood cell production, lymphocytic lineage expansion (T cells, B cells, NK cells, and non-NK ILCs), and the m6A crosstalk with the onco-metabolic prospects of leukemogenesis. Together, an encompassing body of evidence highlighted the emerging m6A significance in the regulation of HSC biology and leukemogenesis.
Space exposure test of hardwood specimens in the International Space Station
Authors: Murata Koji , Nakamura Masashi , Kariya Kenji , Tsuchiya Morio , Yamashiki Yosuke Alexandre , Doi Takao
Published: November 2024

To design a wooden satellite and select the appropriate wood species, we conducted a space exposure test of wood on the International Space Station (ISS). The space exposure test used the Japanese Experiment Module “Kibo” on the Exposed Experiment Bracket Attached on i-SEEP (ExBAS), which is connected to the exposed facility of the ISS. Three wood species considered candidates for the structure of a wooden satellite were used in the exposure test: honoki (Magnolia obovata), yamazakura (Cerasus jamasakura), and dakekanba (Betula ermanii). In the outgassing test conducted in preparation for the space exposure test, the collected volatile condensable materials (CVCM) of the three wood species were below the critical value; however, the total mass loss (TML) exceeded the critical value. However, this was not a problem, because most of the mass loss was due to water evaporation. A space exposure test was conducted for 10 months in 2022. Consequently, no change was observed in the weight of the exposed specimen, and no erosion due to atomic oxygen (AO) was observed. The exposure test was scheduled to be carried out in the forward direction of the ISS travel; however, owing to equipment trouble, it was installed in the opposite direction. This is because an insufficient AO flux collided with the specimen. In terms of wood color, the brightness of the three species decreased owing to space exposure, and saturation increased in two wood species, except for yamazakura. A thin-film layer was observed on the surface of the aluminum cover to which the exposed specimen was fixed, which may have also formed on the surface of the wood specimen, reducing its brightness. However, because the hue angle did not change significantly, only a slight deterioration of the surface layer owing to vacuum ultraviolet was observed. The results confirmed that the wood had hardly deteriorated in space for approximately 10 months.
Public perceptions of the Hippocratic Oath in the U.K. 2023
Authors: Green Ben
Published: November 2024

This paper explores public perceptions of the Hippocratic Oath (Physician’s Oath) in the U.K. Results of a questionnaire administered online to 106 adults indicated that the majority were of the opinion that their primary and secondary health care doctors had taken the Oath (88% and 86% respectively). A majority thought that nurses, paramedics, psychotherapists and graduate scientists and researchers should also take some form of professional oath. Elements of the Oath which were deemed most important included that it is a sworn oath, that doctors should not harm patients, act in the best interests of patients, abide by the principles of autonomy and informed consent and maintain patient confidentiality. A significant proportion - about 20% - of the UK public felt that doctors had forgotten their Hippocratic Oath during COVID-19 lockdowns and associated vaccination programme, suggesting that recent history may have damaged the public faith in the medical profession.
Diagnostic accuracy of the point-of-care standard G6PD test™ (SD Biosensor) for glucose-6-phosphate dehydrogenase deficiency: a systematic review and meta-analysis
Authors: Martínez Juan Camilo , Vélez-Marín Viviana , Lopez-Perez Mary , Patiño-Lugo Daniel F. , Florez Ivan D.
Published: November 2024

Background

Glucose-6-Phosphate Dehydrogenase deficiency (G6PDd) is a common genetic enzymopathy that can induce haemolysis triggered by various factors, including some anti-malarial drugs. Although many Point-of-Care (PoC) tests, such as Standard G6PD™ are available to detect G6PDd, its pooled diagnostic test accuracy (DTA) remains unknown.

Methods

To estimate the DTA of StandG6PD-BS at various thresholds of G6PDd, a systematic review with a DTA meta-analysis were conducted, searching EMBASE, MEDLINE, and SciELO databases up to April 4, 2024.The included studies were those that measured G6PD activity using StandG6PD-BS (reference test) and spectrophotometry (gold standard) in patients suspected of having G6PDd. The risk of bias (RoB) of the studies was assessed using the QUADAS-2 tool and the certainty of evidence (CoE) with the GRADE approach. For the estimation of within-study DTA, a random-effect bivariate meta-analysis was performed to determine the pooled sensitivity and specificity for 30%, 70%, and 80% enzyme levels’ thresholds, and a graphical analysis of the heterogeneity using crosshair and Confidence Regions on receiver operating characteristic (ROC) space plots.

Results

After screening 2496 reports, four studies were included with 7864 participants covering all thresholds. Two studies had high RoB in QUADAS-2 domains 2 and 3, and the others had low RoB, with low, moderate, and high heterogeneity at the 30%, 70%, and 80% thresholds, respectively. The pooled sensitivity was 99.1%, 95.7%, and 90% for 30%, 70%, and 80% thresholds, respectively. The pooled specificity was 97.4%; 92.9%; and 89.0% for 30%, 70%, and 80% thresholds, respectively.

Conclusion

StandG6PD-BS is a PoC test with high sensitivity and specificity to detect G6PDd at different thresholds.
Microfluidic and impedance analysis of rosemary essential oil: implications for dental health
Authors: Joseph Karunan , Petrović Bojan , Ibrahim Syarifah Aisyah Syed , Thiha Aung , Milić Lazar , Ahmad Mohd Yazed , Pavlović Nebojša , Kojić Sanja , Ibrahim Fatimah , Stojanović Goran M.
Published: November 2024

Background

Oral health is closely linked to systemic conditions, particularly non-communicable diseases (NCDs), which can exacerbate oral issues. Essential oils (EOs) have emerged as potential alternatives for oral health due to their antibacterial, anti-inflammatory, and antioxidant properties. Among these, rosemary essential oil (REO) shows promise due to its various biological activities. This study investigates the potential of REO in dental applications using microfluidic devices and electrochemical impedance spectroscopy (EIS) to analyze the electrical properties of REO in artificial saliva (AS) mixtures.

Results

The study demonstrated significant variations in impedance across different REO concentrations and their mixtures with AS. Higher impedance was observed in REO mixtures, particularly at lower frequencies, indicating distinct electrical properties compared to pure AS. The impedance of REO was influenced by its concentration, with a 1% REO solution showing higher impedance than a 4% solution, possibly due to micelle formation and changes in dielectric properties. Additionally, microfluidic devices enabled precise control over fluid interactions and real-time monitoring, offering valuable insights into REO's behavior in a simulated oral environment. The impedance data demonstrated significant differences in REO–AS mixtures, highlighting potential interactions critical for oral care applications.

Conclusions

Rosemary essential oil exhibits unique electrical properties, making it a promising candidate for dental applications, particularly in preventing and treating oral diseases. Microfluidic devices enhance the accuracy and reliability of studying REO's interactions with AS, providing a robust platform for future dental research. The findings suggest that REO could be effectively incorporated into oral care products, offering a natural alternative for combating oral pathogens, reducing inflammation, and protecting against oxidative stress. Future research should focus on clinical trials to validate these findings and explore the synergistic effects of REO with other essential oils.
Epigenetic signatures of asthma: a comprehensive study of DNA methylation and clinical markers
Authors: Van Asselt Austin J. , Beck Jeffrey J. , Finnicum Casey T. , Johnson Brandon N. , Kallsen Noah , Viet Sarah , Huizenga Patricia , Ligthart Lannie , Hottenga Jouke-Jan , Pool René , der Zee Anke H. Maitland-van , Vijverberg S. J. , de Geus Eco , Boomsma Dorret I. , Ehli Erik A. , van Dongen Jenny
Published: November 2024

Background

Asthma, a complex respiratory disease, presents with inflammatory symptoms in the lungs, blood, and other tissues. We investigated the relationship between DNA methylation and 35 clinical markers of asthma.

Methods

The Illumina Infinium EPIC v1 methylation array was used to evaluate 742,442 CpGs in whole blood from 319 participants from 94 families. They were part of the Netherlands Twin Register from families with at least one member suffering from severe asthma. Repeat blood samples were taken after 10 years from 182 individuals. Principal component analysis on the clinical asthma markers yielded ten principal components (PCs) that explained 92.8% of the total variance. We performed epigenome-wide association studies (EWAS) for each of the ten PCs correcting for familial structure and other covariates.

Results

221 unique CpGs reached genome-wide significance at timepoint 1 after Bonferroni correction. PC7, which correlated with loadings of eosinophil counts and immunoglobulin levels, accounted for the majority of associations (204). Enrichment analysis via the EWAS Atlas identified 190 of these CpGs to be previously identified in EWASs of asthma and asthma-related traits. Proximity assessment to previously identified SNPs associated with asthma identified 17 unique SNPs within 1 MB of two of the 221 CpGs. EWAS in 182 individuals with epigenetic data at a second timepoint identified 49 significant CpGs. EWAS Atlas enrichment analysis indicated that 4 of the 49 were previously associated with asthma or asthma-related traits. Comparing the estimates of all the significant associations identified across the two time points yielded a correlation of 0.81.

Conclusion

We identified 270 unique CpGs that were associated with PC scores generated from 35 clinical markers of asthma, either cross-sectionally or 10 years later. A strong correlation was present between effect sizes at the 2 timepoints. Most associations were identified for PC7, which captured blood eosinophil counts and immunoglobulin levels and many of these CpGs have previous associations in earlier studies of asthma and asthma-related traits. The results point to a robust DNA methylation profile as a new, stable biomarker for asthma.
Accurate prediction of protein–ligand interactions by combining physical energy functions and graph-neural networks
Authors: Hong Yiyu , Ha Junsu , Sim Jaemin , Lim Chae Jo , Oh Kwang-Seok , Chandrasekaran Ramakrishnan , Kim Bomin , Choi Jieun , Ko Junsu , Shin Woong-Hee , Lee Juyong
Published: November 2024

We introduce an advanced model for predicting protein–ligand interactions. Our approach combines the strengths of graph neural networks with physics-based scoring methods. Existing structure-based machine-learning models for protein–ligand binding prediction often fall short in practical virtual screening scenarios, hindered by the intricacies of binding poses, the chemical diversity of drug-like molecules, and the scarcity of crystallographic data for protein–ligand complexes. To overcome the limitations of existing machine learning-based prediction models, we propose a novel approach that fuses three independent neural network models. One classification model is designed to perform binary prediction of a given protein–ligand complex pose. The other two regression models are trained to predict the binding affinity and root-mean-square deviation of a ligand conformation from an input complex structure. We trained the model to account for both deviations in experimental and predicted binding affinities and pose prediction uncertainties. By effectively integrating the outputs of the triplet neural networks with a physics-based scoring function, our model showed a significantly improved performance in hit identification. The benchmark results with three independent decoy sets demonstrate that our model outperformed existing models in forward screening. Our model achieved top 1% enrichment factors of 32.7 and 23.1 with the CASF2016 and DUD-E benchmark sets, respectively. The benchmark results using the LIT-PCBA set further confirmed its higher average enrichment factors, emphasizing the model’s efficiency and generalizability. The model’s efficiency was further validated by identifying 23 active compounds from 63 candidates in experimental screening for autotaxin inhibitors, demonstrating its practical applicability in hit discovery.

Scientific contribution

Our work introduces a novel training strategy for a protein–ligand binding affinity prediction model by integrating the outputs of three independent sub-models and utilizing expertly crafted decoy sets. The model showcases exceptional performance across multiple benchmarks. The high enrichment factors in the LIT-PCBA benchmark demonstrate its potential to accelerate hit discovery.
MAGqual: a stand-alone pipeline to assess the quality of metagenome-assembled genomes
Authors: Cansdale Annabel , Chong James P. J.
Published: November 2024

Background

Metagenomics, the whole genome sequencing of microbial communities, has provided insight into complex ecosystems. It has facilitated the discovery of novel microorganisms, explained community interactions and found applications in various fields. Advances in high-throughput and third-generation sequencing technologies have further fuelled its popularity. Nevertheless, managing the vast data produced and addressing variable dataset quality remain ongoing challenges. Another challenge arises from the number of assembly and binning strategies used across studies. Comparing datasets and analysis tools is complex as it requires the quantitative assessment of metagenome quality. The inherent limitations of metagenomic sequencing, which often involves sequencing complex communities, mean community members are challenging to interrogate with traditional culturing methods leading to many lacking reference sequences. MIMAG standards aim to provide a method to assess metagenome quality for comparison but have not been widely adopted.

Results

To address the need for simple and quick metagenome quality assignation, here we introduce the pipeline MAGqual (Metagenome-Assembled Genome qualifier) and demonstrate its effectiveness at determining metagenomic dataset quality in the context of the MIMAG standards.

Conclusions

The MAGqual pipeline offers an accessible way to evaluate metagenome quality and generate metadata on a large scale. MAGqual is built in Snakemake to ensure readability and scalability, and its open-source nature promotes accessibility, community development, and ease of updates. MAGqual is built in Snakemake, R, and Python and is available under the MIT license on GitHub at https://github.com/ac1513/MAGqual.
Detecting latent subpopulations in international large-scale assessments by fitting MixIRT models using NUTS
Authors: AlHakmani Rehab , Sheng Yanyan
Published: November 2024

The focus of this study is to use the mixture item response theory (MixIRT) model while implementing the no-U-turn sampler as a technique for investigating the presence of latent classes (i.e., subpopulations) among eighth-grade students who were administered TIMSS 2019 mathematics subtest in paper format from the gulf cooperation council (GCC) countries. One-, two-, and constrained three-parameter logistic MixIRT models with one to four classes were used to fit to the data, where the model data fit was assessed using Bayesian fit indices. The results indicate that multiple latent classes or subpopulations can better reflect the mathematical proficiency of eighth graders from the four GCC countries, and specifically the two-class constrained three-parameter MixIRT model provides a relatively better fit to the data. The results also indicate that when a mixture of several latent classes present, the conventional unidimensional IRT model is limited in providing information for multiple latent classes and shall be avoided. In addition to adding to the existing literature on MixIRT models for international large-scale assessments such as TIMSS on its heterogenous subpopulations from a fully Bayesian approach, this study sheds light on the limitation of conventional unidimensional IRT models and subsequently directs attention to the use of the more complex MixIRT model for such assessments.
Simulation of light environments inside lunar holes and their associated subsurface caverns
Authors: Ikeya Kodai , Haruyama Junichi , Miyake Wataru , Tanaka Makoto , Toyokawa Kosei , Nozawa Hitoshi
Published: November 2024

Vertical holes of several tens of meters in diameter and depth have been discovered on the Moon, potentially serving as skylights into subsurface volcanic caverns resembling lava tubes. Because of their scientific importance and potential for use as future lunar bases, these lunar holes and caverns are expected to be targets of intensive exploration in the near future. Using numerical simulations, this study investigates the light environment within a directly and/or indirectly sunlit subsurface cavern accessed through a skylight hole. We specifically analyze the Mare Tranquillitatis Hole (MTH), one of the largest lunar vertical holes, situated near the Moon's nearside meridian. The floor and walls of the hole, and the walls and ceiling of its associated subsurface cavern are lit by reflected sunlight from other parts of the hole and cavern, such as the floors, depending on the solar elevation angle. Furthermore, this paper presents estimation results for camera imaging for a case in which solar elevation angle is 45° in the morning, which is appropriate timing for exploration. Assuming reflectance of 0.1 for the lunar hole and cavern surfaces, we estimate the incident energy onto each pixel of a camera as it descends to the hole's floor. We specifically simulate conditions for a camera with a 12-bit dynamic range, similar to the wide-angle optical navigation camera (ONC-W) onboard the Hayabusa 2 spacecraft. The results suggest that a camera with a fixed gain would struggle to capture both directly and indirectly sunlit areas simultaneously, without saturating bright areas and ensuring minimum incident energy resolution (say, a 10 digital number) for darker areas. To overcome this challenge, adjusting the camera gain based on the hole's and cavern's illumination conditions is necessary.

Graphical abstract
Influence of political stability on the stock market returns and volatility: GARCH and EGARCH approach
Authors: Alim Wajid , Khan Naqib Ullah , Zhang Vince Wanhao , Cai Helen Huifen , Mikhaylov Alexey , Yuan Qiong
Published: November 2024

Political instability has increased drastically in Pakistan during the last few decades. This may intensify the fear of investors and eventually affect investment decisions. Therefore, the stock market’s reaction to political stability must be explored and appropriate policy measures should be prescribed. This paper examines the effect of political stability on stock market returns and volatility using time series data from Pakistan for the period from January 02, 2004, to May 31, 2018. The study uses the Generalized Autoregressive Conditional Heteroskedastic (GARCH) and Exponential Generalized Autoregressive Conditional Heteroskedastic (EGARCH) models to achieve the main objectives. The findings of the GARCH and EGARCH models confirm that political stability has a positive and significant influence on both stock market returns and volatility in Pakistan. In addition, the EGARCH findings indicate that negative shock (political instability) creates more volatility in the stock market than positive shock (political stability). The results of this study reveal that financial market investors are affected more by political instability. In conclusion, this study suggests that political stability is an essential factor affecting financial market volatility, and it leads to stable financial markets. It also emphasizes that political instability needs to be resolved to achieve stable financial markets.
Thinking alike, acting alike? An assessment of organizational homophily and paradigmatic pragmatism in networks for local integration policies
Authors: Elien Diels , Stijn Oosterlynck
Published: November 2024

As migrant integration policies touch upon almost all spheres of citizens’ life, collaboration across policy levels as well as across policy domains is needed. Over time, a series of both policy and analytical paradigms on the integration of migrants emerged, going from the traditional ideas of differentialism and assimilation, over multiculturalism, interculturalism, integrationism, liberal egalitarianism and mainstreaming, up to superdiversity and intersectionality. While networks for integration policies are likely to be at least partly driven by these paradigms, recent studies seem to nuance this assumption based on so called paradigmatic pragmatism, meaning that cities draw on a variety of ideas and pragmatically combine them under the header of diversity policies. The need for multi-level and multi-actor governance on migrant integration, the theoretical knowledge about homophily in organizational networks, and the recent findings on paradigmatic pragmatism, raise the question to what extent collaboration between public and non-profit organizations active around migrant integration is based on a shared policy paradigm on the integration of migrants. This question is answered based on a multiple case study of four central cities in Flanders (Belgium), covering 1.403 organizations. In the quantitative and qualitative (social network) analyses, comparisons along both the axes of cities and policy domains are made. Findings contribute to literature on organizational homophily, showing that clustering of organizations is likely to be at least partly driven by policy paradigm on integration. This is however nuanced by the observation that policy paradigms appear to be ambiguous, which in turn reveals opportunities for more flexible collaborations between actors and for avoiding deadlocks in policy making.
Occurrence and exposure to polycyclic aromatic hydrocarbons (PAHs) in traditional dry-cured or smoked meat products from Brazil
Authors: da Silva Simone Alves , de Rossi Gustavo Zanetti , de Almeida Adriana Palma , Guizellini Glória Maria , Torres Elizabeth Aparecida Ferraz da Silva , Rogero Marcelo Macedo , Sampaio Geni Rodrigues
Published: November 2024

Polycyclic aromatic hydrocarbons (PAHs) are classified as environmental and food contaminants, with several adverse health effects, especially genotoxic and carcinogenic actions. In processed meats, they are the major contaminants, especially those subjected to smoking processes. Considering that the population is exposed to PAHs through several routes, and that the ingestion of contaminated food is considered the main one, evaluating the concentrations of these contaminants in food becomes essential, as well as the exposure and risk to the population at different ages through ingestion of the evaluated products. In the current study, the levels of nine PAHs (PAH9) were measured in 205 processed meat products commercially available and with high consumption in Brazil. The methodology involved saponification, extraction with n-hexane, purification with solid-phase extraction (SPE) silica cartridges, and quantification by liquid chromatography with fluorescence detection. In general, 83% of all samples were contaminated with at least one of the studied PAHs, and the measured PAH9 levels ranged between < LOQ-108.24 µg/kg. The highest mean of total PAHs was found in smoked sausage (108.24 μg/kg), while the lowest content was found in ham (1.83 μg/kg). Benzo[a]pyrene (BaP) and PAH4 (benz[a]anthracene, chrysene, benzo[b]fluoranthene, and BaP) exceeded the maximum permissible limits of the European Union (EU) in three (1.5%) and 18 samples (8.7%), respectively. The results of margin of exposure results (MOE ≥ 10,000) and incremental lifetime cancer risk (ILCR) values (10 − 6 < ILCR < 10 − 4) in all ten types of meat indicated there were low significant potential health problems related to meat products consumption for the Brazilian population.

Graphical Abstract
Unlocking the sustainable role of melatonin in fruit production and stress tolerance: a review
Authors: Himanshu  , Sharma Sunny , Rana Vishal Singh , Ankit  , Thakur Vikanksha , Kumar Arun , Prachi  , Thakur Shivender , Sharma Neha
Published: November 2024

While melatonin, a vital player in plant physiology, initially attracted recognition due to its involvement in animal circadian rhythms, the molecule appears to be a multifunctional molecule requiring substantial attention for prospective applications in sustainable horticulture. It has been identified and recorded in numerous fruit crops, and its significance in physiological functions is critical for crop productivity. It is critical in safeguarding plants in response to reactive oxygen species in oxidative stress, one of the most damaging stressors to plant life in adverse conditions. Melatonin also cooperates with plants in boosting stress resistance, which concerns abiotic stress factors, e.g. low and high temperature, drought stress, toxicity of heavy metals, and biotic stress factors, including pests and pathogens. The anti-senescence properties of melatonin in aging leaves may be explained by its widespread antioxidant activity and its function in maintaining chlorophyll. The function of melatonin in controlling the production of genes linked to ethylene to modify postharvest fruit ripening has been the subject of an astounding amount of research. Additionally, recent research has shown that melatonin works with other phytohormones and well-known chemicals like nitric oxide and reactive oxygen species to assist plants in responding to biotic stress.The present review emphasizes a perspective that examining the role of melatonin in fruit crop physiology and stress responses may be a promising research direction in prospective fruit crop yield. In particular, this perspective is well supported by the following: melatonin is involved in the antioxidant response of fruit crops and can thus be used to mitigate the stressful impact of various environmental conditions; melatonin influences the development of plants and, consequently, affects fruit yield and quality; and applying melatonin is feasible for mitigating the impact of abiotic factors, such as cold, drought, heavy metals, and biotic factors, pests, and pathogens.
Navigating Gatekeeping Challenges in Pediatric and Young Adult Palliative Oncology and End-of-Life Research
Authors: Ananth Prasanna , Snaman Jennifer M.
Published: November 2024

Participation in research offers families a sense of control and meaning in pediatric cancer care. Gatekeeping limits progress—collaboration is key. #PediatricOncology #PalliativeCare #Research #PallOnc #pedonc #hpm #hapc
Severe Hypertriglyceridemia in a Patient Treated With Sirolimus for Graft-vs-Host Disease Prophylaxis
Authors: Criner Kristin , Student Jeffrey , Arkin Jacob , Carp Julia , Sokoloff Samantha
Published: November 2024

Hypertriglyceridemia is an important and well documented adverse effect caused by the immunosuppressive agent sirolimus. Patients treated with sirolimus require frequent monitoring of blood lipid panels and prompt treatment with appropriate triglyceride-lowering therapies. We report the case of an asymptomatic 65-year-old female stem cell transplant recipient who developed extreme hypertriglyceridemia with levels > 19,000 mg/dL (214 mmol/L) (reference range, < 150 mg/dL [< 1.7 mmol/L]), secondary to sirolimus for prophylaxis of graft-vs-host disease. Acute treatment included admission to the intensive care unit for initiation of an intravenous insulin infusion, low-fat diet, and discontinuation of sirolimus. These measures, in addition to initiation of oral triglyceride-lowering agents and improved glycemic control, led to substantial improvement in triglyceride levels.
Pembrolizumab-induced Thyroiditis, Hypophysitis and Adrenalitis: A Case of Triple Endocrine Dysfunction
Authors: Rossi Silvia , Silvetti Francesca , Bordoni Monia , Ciarloni Alessandro , Salvio Gianmaria , Balercia Giancarlo
Published: November 2024

Immune checkpoint inhibitor drugs can trigger autoimmune endocrine reactions as a known side effect. Several cases of immunotherapy-induced autoimmune endocrinopathies have been described, but multiple sequential endocrine toxicities are a rare occurrence. A 39-year-old patient with metastatic melanoma started adjuvant therapy with pembrolizumab. One month later he presented with asymptomatic thyrotoxicosis and, within several weeks, overt hypothyroidism, for which he started levothyroxine therapy. Subsequently the patient developed central adrenal insufficiency due to probable hypophysitis, and steroid replacement therapy was started. Pembrolizumab therapy was then discontinued. After a few months, a full recovery of pituitary function was observed, but primary adrenal insufficiency occurred, requiring additional fludrocortisone therapy. The described clinical case is a very uncommon case of triple endocrinological toxicity from immunotherapy. The clinical and biochemical manifestations of immunotherapy-induced endocrinopathies can be variable and atypical; therefore, it is necessary to pay special attention to any clue of hormonal dysfunction.
Detection of viral infectious agents causing calf diarrhea on dairy farms in Serbia
Authors: Milicevic V , Kureljušić B , Glišić D , Veljović L , Maletić J , Jezdimirović N , Maletić M
Published: November 2024

Neonatal calf diarrhea (NCD) is a multifactorial disease commonly occurring in unweaned calves, and one of the major causes of calflosses worldwide. In this study, we investigated the role of viral agents in neonatal calf diarrhoea from dairy farms with reported outbreaks in the winter of 2021-2022.For this study, we tested rectal swab samples from 20 commercial dairy farms with 100-1000 cows with reported acute diarrhoea in neonatal calves.From each farm, five to ten calves, up to 2 months of age, in total 154, with acute diarrhea were sampled by rectal swabbing. Out of 20 tested dairy farms, at 4 farms none of the testedviruses was detected whereas at least one of the tested viruses was detected at 16 farms (80%). As the most prevalent, mono BRV infection was detected at 10 farms (62.5%), followed by a single BCoV infection at 3 farms (18.75%). Coinfection of BVDV and BRV was demonstrated at 2 farms (12.5%), and simultaneous infection with BVDV, BRV and BCoV at one farm (6.25%). G typing of BRV revealed that 60% of the BRVs were G10, 33.3% were G6, and 16.7% were G8. P typing indicated that 66.7% of the BRVs were P[11] and that 33.3% were P[5]. This study revealed viral causative agents being the most often found in diarrheic neonatal calves in Serbia, thuscontributing to the development of more effective strategies for disease prevention and control.
Oxidative stress markers in canine parvovirus infection
Authors: Khoshvaghti A , Baziyari Ν , Dehghani M , Vaziri F
Published: November 2024

Parvovirus infections are among the deadly viral diseases in dogs and sick puppies. Young dogs are more prone to the virus. Antioxidants are important components of the defense system which prevent cell damage by neutralizing reactive oxygen species. Malondialdehyde is a reliable and commonly used marker of oxidative stress. The present study was performed to determine the role of oxidative stress markers in canine parvovirus disease.
Thirty Cane Corso male dogs with an average age of 3-6 months were enrolled in this study out of which 10 animals were spontaneously infected with the parvovirus and the other 10 were healthy. The activity of superoxide dismutase (SOD), total antioxidant capacity (TAC), and malondialdehyde were measured by colorimetric methods whereas the enzymatic activity of glutathione peroxidase (GPX) was measured. Catalase was measured by means of rapid spectrophotometry and chromography was used for obtaining the concentrations of vitamin D and vitamin E.
The increase in malondialdehyde in infected dogs demonstrates that oxidative stress plays a role in the pathogenesis of the parvovirus. The significant elevation in TAC, and lack of important changes in superoxide dismutase and catalase are indicative of a good response by the antioxidant system against oxidative stress induced by the parvovirus. In conclusion, oxidative stress is involved in the pathogenesis of parvovirus. Thus, strengthening the antioxidant system can be effective in the prevention and treatment of canine parvovirus infection.
Tomasza z Akwinu obrona doktryny czyśćca
Authors: Zatwardnicki Sławomir
Published: November 2024

W artykule wskazano najpierw, że Tomasz z Akwinu prowadził dyskusję w oparciu o wspólnie dzielone z adwersarzem przekonania. Stąd w polemice z chrześcijanami odwoływał się do autorytetu Pisma Świętego, czego przykładem może być argumentacja w De rationibus fidei na rzecz czyśćca adresowana do Greków i Ormian wyznających błędną naukę co do stanu dusz po śmierci. Celem artykułu było po pierwsze ukazanie toku rozumowania na rzecz czyśćca, w tym zwłaszcza sposób, w jaki magister in sacra pagina skorzystał z tekstów natchnionych w swojej argumentacji w dziełku oraz jak teksty biblijne, które odegrały rolę w jego polemice, były przez niego wyjaśniane w komentarzach biblijnych. Po drugie, chodziło również o ukazanie wzajemnego związku między dziewiątym rozdziałem Jak uzasadniać wiarę a komentarzami biblijnymi, oraz określenie, czy argumentacja Doktora anielskiego pozostaje przekonująca bez sięgnięcia po te komentarze. W tym sensie artykuł wpisuje się w nurt tomizmu biblijnego. I po trzecie, zamiarem autora artykułu było również wyłonienie cech charakterystycznych dla Tomaszowej egzegezy, jakie dały o sobie znać w De rationibus fidei.
Editorial
Authors: Martinez Enrique , Prieto Lucas , Manresa Ignacio M.
Published: November 2024

To commemorate the jubilee of the death and canonization of St. Thomas Aquinas, the Santo Tomás Institute of Balmesiana organized the 10th Symposium of Thomistic Studies, St. Thomas Aquinas, Master of the Sacred Page. The event took place at the Abat Oliba CEU University in Barcelona from May 10th to 12th, 2024. Other institutions, in particular, the Association pour le Centenaire Thomas d’Aquin (ACTA) from Toulouse, contributed greatly to the organization of the symposium.
This event aimed to explore St. Thomas’ use of Sacred Scripture in his role as a biblical scholar. This is one of the aspects of the doctrine of Aquinas that has recently gained prominence, as there has been a renewed appreciation for the central role that Sacred Scripture played in the theology of the Angelic Doctor, frequently referred to as “biblical Thomism”. The purpose of the symposium was not only historical but also doctrinal, aiming to shed light on contemporary biblical hermeneutics and exegesis.
The symposium was structured around the three core activities of the medieval scholar: legere, disputare, and praedicare, with the intention to understand the place of Sacred Scripture in each of these. It began with several introductory issues that addressed Aquinas' biblical hermeneutics and its foundations, with enriching contributions from professors Martínez, Amado, Loiseau, and Manresa.
Next, the symposium delved into Aquinas' biblical exegesis, focusing on his commentaries on St. John, St. Paul, and a book of the Old Testament, with insightful presentations by Professors Giambrone, Roszak, and Ossandón.
The following sessions were dedicated to the examination of how St. Thomas utilized disputare in his construction of a theological system. Professors Margelidon, Aroztegi, and Prieto offered valuable reflections on grace, the sacraments, and divine knowledge.
Finally, the symposium turned to Aquinas' praedicare and his use of Scripture in preaching, with illuminating presentations by professors Smith, Vijgen, and Perrin. Thomas Olivier Venard concluded the symposium by discussing the contemporary relevance of Aquinas' biblical hermeneutics.
We are pleased to offer the reader a substantial number of these enriching contributions in both this and the next issue of Bíblica et Patristica. We are grateful to the Editorial Board for their willingness to publish the texts and, in this way, familiarise the readers with the fruitful discussions we experienced in Barcelona in May.
Drugs and other substances aggravating acne vulgaris
Authors: Zalewa Karolina , Olszak Joanna , Kapłan Wojciech , Orłowska Dominika , Bartoszek Lidia
Published: November 2024

Introduction and Purpose
This review explores several factors exacerbating acne, including the supplementation of vitamins B6 and B12, the impact of red ginseng oil, dairy and whey product consumption, iodine association, anabolic-androgenic steroids, alcohol abuse, and the influence of progestin contraceptives.
Material and methods
This review is based on articles from the PubMed database, covering the years 2018-2023, using keywords: acne vulgaris, acne vulgaris aggravation, substances aggravating acne vulgaris.
Results High doses of B6 and B12 have been reported to worsen acne, potentially linked to their prolonged use. Red ginseng oil, believed to have numerous health benefits, may exacerbate acne symptoms by increasing inflammatory biomarkers. Dairy consumption, specifically casein and whey proteins, is associated with increased insulin-like growth factor 1, contributing to acne aggravation. Iodine has been extensively linked to acneiform eruptions, and its correlation with dairy consumption is hypothesized. Anabolic-androgenic steroids, found in muscle-building supplements, elevate sebum production and may cause acne fulminans. Alcohol abuse further intensifies acne symptoms by impacting testosterone levels, promoting proinflammatory cytokine production, and altering the skin microbiome. Progestin contraceptives, particularly levonorgestrel and etonorgestrel, exhibit androgenic properties that may increase sebum production and potentially aggravate acne.
Conclusions Understanding and identifying these exacerbating factors are crucial for healthcare providers to enhance anti-acne therapy outcomes, emphasizing the importance of not only treating but also preventing the escalation of acne manifestations in patients.
Nowa perspektywa. Leksykograficzne badania nad afektem w operze przełomu XVIII i XIX w.
Authors: Falentin Kamil
Published: November 2024

Recenzja: Małgorzata Lisecka, Głos – ciało – scena. Afektywność teatru operowego we francusko-włoskim dyskursie słownikowym 1768–1826, Seria Monografie Fundacji na rzecz Nauki Polskiej, Wydawnictwo Naukowe UMK, Toruń 2023.
To catch the uncatchable/to capture the intangible. Reflections on selected problems of safeguarding Intangible Cultural Heritage. The Example of the Koniaków lace making skills
Authors: Czerwińska Kinga
Published: November 2024

The 2003 UNESCO Convection for the Safeguarding of the Intangible Cultural Heritage has given impetus to intensive work on the disappearance of cultural specificity in many countries. As a result of taking safeguarding measures taken for expressions of intangible cultural heritage, the necessity to redefine existing practices in this matter has also shown up. The text refers to reflection on selected problems of documentation and popularisation of intangible cultural heritage (ICH), with particular regard to the example of the Koniaków lace making skills. This handicraft, despite the changing reality, has been an uninterrupted manifestation of the region's tradition for more than 100 years. Local lace makers adapt their forms to new emerging needs. The educational or commercial activity concentrated around lace makes these skills an important bonding element for the local community. The lively activity of dissemination and promotion of the Koniaków lace brought an entry to UNESCO's National List of Intangible Cultural Heritage in 2017.
Erratum on “Purchase Decision Reversals” Model by Shulman et al. (2015)
Authors: He Xin , Li Chuntao
Published: November 2024

This is an erratum on the paper “Consumer uncertainty and purchase decision reversals: Theory and evidence”, Marketing Sci. 34(4):590–605.
Political communication strategies of local politicians in the political constellation of legislative elections at the Regency/City level
Authors: Nurhidayah Sitti , Fadillah Dani
Published: November 2024

This research aims to determine how local politicians carry out the political communication strategy to win the political constellation of district/city-level legislative elections. This research uses a qualitative approach; in collecting data, researchers chose politicians from the Gerindra party named Muhasbih, who fought in the Polewali Mandar district legislative elections in the 2024 elections, as research subjects. Researchers conducted in-depth interviews with the politician and his success team, who actively participated in winning the 2024 election. The results of this study indicate that effective communication in winning political constellations in legislative elections at the district/city level requires understanding the audience and local political dynamics. Through in-depth interviews with politicians and their success teams, it was found that the success of political communication strategies lies in delivering relevant, measurable, and understandable messages to voters. In addition, it was found that the presence of politicians in the field and direct interaction with voters is very influential in building personal relationships and trust. This suggests that the human side and direct involvement in local politics play a crucial role in creating emotional bonds between politicians and voters, which can influence voters' decisions inside the voting booth. Furthermore, the results of this study also highlight the need for adopting strategies that are dynamic and responsive to political and technological developments. Although local politicians such as Muhasbih do not use social media in their campaigns, it is important to continuously monitor trends and adapt political communication strategies as needed as the political environment changes.
The Effect of Helping Skill Preceptor Education Program on the Therapeutic Helping Skill of Nurse
Authors: Ahn Hwang Ran , Sim Young Suk
Published: November 2024

The purpose of this study was to examine type of helping skill of nurses and the effect of the helping skill preceptor education program on helping skill. To acomplish the purpose of this study, the hypothesis of this were establish as follows：The nurse which were given nursing counselling training program will higher in score of therapeutic helping skill, but nontherapeutic helping skill (evaluation, interpretation, sympathy, examine, solving type) were lower than before. The subject of this study were 39 nurse of G university hospital who volunteered to participate in this program. The subjects received training program for 12hours. The instrument used in this study was Sul(1990)’s helping skill measured scale. The statistical methods used means and Paired t-test. Paired t-test was run with SPSS/PC+ WIND0WS( version 9.0) program.The conclusions based on the results are as follows:1. The score about type of helping skill of preceptor nurses were therapeutic (6.92).nontherapeutic (2.97). The scores of type of nontherapeutic helping skill were examine ( .95) sympathy ( .69). solving( .61),interpretation (.35), evaluation( .35) ordinary.2. The helping skill preceptor education program for preceptor nurses is effective for increasing therapeutic helping skill(p<.001), and decreasing nontherapeutic skill(p<.001).
Factors Influencing on School Violence in Middle School Students
Authors: Kim Myung Jd
Published: November 2024

The purpose of this study is to identify factors which influence on school violence and to provide a basis for school violence prevention within the context of social ecology.This model was constructed based on the Riner's Social Ecology Model of Adolescent Interpersonal Violence Prevention and the literature review of school violence.The study is designed to evaluate all the variables at the same time. Empirical data was collected by self - reported questionnaires from 646 middle school students in Jeonju, from Dec.9. 2000 through Dec. 19, 2000. The reliability of the 21 instruments was tested with Cronbach's alpha which ranged from .634 to .876.The data was analyzed using a SAS Program for descriptive statistics, Pearson Correlation Coefficients and stepwise multiple regression.The results are as follows:1. The mean of the bullied score on the school violence scale was 14.80 and the victimized score was 14.53. A descriptive analysis shows that the bullying experiences were 84.4% and victimized experiences were 81.4%. 2. There were signigicant correlations among all the predictive variables and the bullying experience(r= .40.23, p<.05).The experience of victimization showed a significant correlation between family climate, community climate, anger, self匕efficacy, peer influence and bullying experiences (r= .54--.18, p<.05).3. Stepwise multiple regression analysis showed that:1) The most influential factor on bullying is the experience of victimization and accounts for 29.7% of the variance in bullying experience, peer influence, anger, community climate and family climate accounted for 7.8% 3.4%, 1.7%, 0.8% respectively and these variables combined accounted for 43.3% of the variance in the bullying experience.2) The bullying experience was the main influential factor of the experience of victimization and accounted for 29.7% of the variance in the experience of victimization.Self-efficacy, community climate and anger accounted for 1.0%, 0.6%, 0.5% respectively and these variables combined accounted for 31.7% of the variance in the experience of victimization.In conclusion, the experience of victimization, bullying experience, anger, self-efficacy, peer influence, community climate, and family climate were identified as an important variables of school violence. A suggestion will be made to establish comprehensive integrated strategies for prevention and intervention to minimize the school violence.