bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Frost Cracks and Large Poplar Borer Damage on Stem Rot in Hybrid Aspen (Populus tremula L. × Populus tremuloides Michx.) Clones
Authors: Čakšs Roberts , Zeltiņš Pauls , Čakša Linda , Zeps Mārtiņš , Jansons Āris
Published: April 2022

Hybrid aspen (Populus tremula L. × Populus tremuloides Michx.) plantations may produce valuable sawlogs for the growing timber market and contribute to carbon sequestration. However, environmental risks such as stem rot, the spread of which is facilitated by insect or frost damage, may reduce the proportion of valuable timber. It is important to understand the various factors affecting the spread of aspen rot to mitigate negative impacts with tree breeding. This study aimed to assess the impact of frost cracks and large poplar borer on stem rot in hybrid aspen clones in two clonal trials in Latvia. Genetic parameters for the traits were also estimated. The presence of insect passages substantially increased the probability of stem rot without distinct clonal differences. A negative and mainly insignificant correlation was observed between rot and stem cracking. The highest broad-sense heritability (H2 = 0.21) and strong site-site genotypic correlation (0.86) showed that the probability of stem rot is genetically determined in the study material. Significant differences in diameter at breast height, the presence of stem rot, and its severity were found among the clones, albeit without undesirable positive correlation between growth and presence of decay. This indicated its potential to improve both productivity and rot resistance.
Challenges in Expression and Purification of Functional Fab Fragments in E. coli: Current Strategies and Perspectives
Authors: Patil Rucha S. , Anupa Anupa , Gupta Jaya A. , Rathore Anurag S.
Published: April 2022

Microbial host systems remain the most efficient and cost-effective chassis for biotherapeutics production. Escherichia coli is often the preferred host due to ease of cloning, scale-up, high product yields, and most importantly, cost-effective cultivation. E. coli often experience difficulties in producing biologically active therapeutics such as Fab fragments, which require protein folding and subsequent three-dimensional structure development. This paper outlines the recent improvements in upstream and downstream unit operations for producing Fab fragments in E. coli. Monoclonal antibody fragments (Fab) are a rising class of biotherapeutics and their production has been optimised using coexpression of molecular chaperones such as DsbC or DnaK–DnaJ–GrpE, as well as strain engineering for post-translational modifications such as disulphide bridging. Different media systems such as EnBase and combining nitrogen source supplementation with low-temperature cultivation have resulted in improvement in cell integrity, protein expression, and protein refolding. The recovery of native proteins from insoluble inclusion bodies can be improved by adjusting refolding conditions, as well as by incorporating multimodal and affinity chromatography for achieving high product yields in purification. Recent developments summarised in this review may tune the E. coli expression system to produce more complex and glycosylated proteins for therapeutic use in the near future.
Wild Saccharomyces Produced Differential Aromas of Fermented Sauvignon Blanc Must
Authors: Mendes Sandra D. C. , Arcari Stefany Grützmann , Werner Simone Silmara , Valente Patricia , Ramirez-Castrillon Mauricio
Published: April 2022

Nine Saccharomyces strains, previously isolated from vineyards in Southern Brazil, were used as starter cultures in fermentations of Sauvignon Blanc (SB) must at laboratory scale, to study inter-strain differences in aroma profiles. The molecular profiles differentiated the following isolates from the reference strain (SC2048), which is typically used in wine production: 06CE, 11CE, 33CE, 01PP, 12M, 13PP, 26PP, 28AD, and 41PP. Under the same conditions, each of these strains produced different concentrations and combinations of metabolites, which significantly influenced the aroma of the fermented SB must. Volatile compounds such as octanoic acid, diethyl succinate, and ethyl lactate were associated with the strains 26PP, 41PP, 01PP, and 12M, while strains 33CE, 28AD, 13PP, and 06CE were associated with the production of ethyl acetate and 1-hexanol. Strain 06CE produced 592.87 ± 12.35 µg/L 1-hexanol. In addition, the olfactory activity values (OAVs; we considered only values >1) allowed us to evaluate the participation of each compound in the aroma of the final fermented SB. In conclusion, the selected wild strains are promising candidates for improving the regional characteristics of wine.
Detecting Fire-Caused Forest Loss in a Moroccan Protected Area
Authors: Castro Iliana , Stan Amanda B. , Taiqui Lahcen , Schiefer Erik , Ghallab Abdelilah , Derak Mchich , Fulé Peter Z.
Published: April 2022

Fire is a concern for the sustainability of dry forests such as those of the Mediterranean region, especially under warming climate and high human use. We used data derived from Landsat and MODIS sensors to assess forest changes in the Talassemtane National Park (TNP) in North Africa from 2003–2018. The Talassemtane National Park is a protected area in northern Morocco, a biodiverse, mountainous region with endemic species of concern such as the Moroccan fir (Abies marocana) and Barbary macaque (Macaca sylvanus). To help the managers of the TNP better understand how the forest has been impacted by fire vs. other disturbances, we combined information from remotely derived datasets. The Hansen Global Forest Change (GFC) data are a global resource providing annual forest change, but without specifying the causes of change. We compared the GFC data to MODIS wildfire data from Andela’s Global Fire Atlas (GFA), a new global tool to identify fire locations and progression. We also analyzed surface reflectance-corrected Landsat imagery to calculate fire severity and vegetation death using Relative Differenced Normalized Burn Ratio analysis (RdNBR). In the park, GFC data showed a net loss of 1695 ha over 16 years, corresponding to an approximately 0.3% annual loss of forest. The GFA identified nine large fires that covered 4440 ha in the study period, coinciding with 833 ha of forest loss in the same period. Within these fires, detailed image analysis showed that GFA fire boundaries were approximately correct, providing the first quantitative test of GFA accuracy outside North America. High-severity fire, as determined by RdNBR analysis, made up about 32% of burned area. Overall, the GFA was validated as a useful management tool with only one non-detected wildfire in the study period; wildfires were linked to approximately 49% of the forest loss. This information helps managers develop conservation strategies based on reliable data about forest threats.
Interrelating Grain Hardness Index of Wheat with Physicochemical and Structural Properties of Starch Extracted Therefrom
Authors: Ni Derang , Yang Fan , Lin Lin , Sun Chongde , Ye Xingqian , Wang Li , Kong Xiangli
Published: April 2022

To investigate the physicochemical, structural, and rheological characteristics of starch from wheat cultivars varying in grain hardness index employed in making jiuqu and to interrelate grain hardness index with physicochemical and structural properties of starch. Starch extracted therefrom was investigated for structural and physicochemical properties. Starch granules showed relatively wide granule size distribution; large size granules showed lenticular shapes while medium and small size granules exhibited spherical or irregular shapes. Starch from wheat with a lower grain hardness index exhibited a relatively higher degree of crystallinity. Chain-length profiles of amylopectin showed distinct differences; among the fractions of fa, fb1, fb2, and fb3 representing the weight-based chain-length proportions in amylopectin, the fa fractions ranged from 19.7% to 21.6%, the fb1 fractions ranged from 44.4% to 45.6%, the fb2 fractions ranged from 16.2% to 17.0%, and the fb3 fractions ranged from 16.1% to 18.8%, respectively. To, Tp, Tc, and ∆H of starch ranged from 57.8 to 59.7 °C, 61.9 to 64.2 °C, 67.4 to 69.8 °C, and 11.9 to 12.7 J/g, respectively. Peak viscosity, hot pasting viscosity, cool pasting viscosity, breakdown, and setback of starch ranged from 127 to 221 RVU, 77 to 106 RVU, 217 to 324 RVU, 44 to 116 RVU, and 137 to 218 RVU, respectively. Both G’ and G” increased in the frequency range of 0.628 to 125.6 rad/s; the wheat starch gels were more solid-like during the whole range of frequency sweep.
Effect of Sour Cherry or Plum Juice Marinades on Quality Characteristics and Oxidative Stability of Pork Loin
Authors: Nour Violeta
Published: April 2022

In this study, the potential of sour cherry and plum juices was evaluated to be used in the traditional marination of meat. Slices of pork loin were marinated for 24 h in brine of 3% NaCl or sour cherry and plum juice marinades containing 60% fruit juice while the control group consisted of non-marinated meat slices. Proximate composition, marinating loss, cooking loss, drip loss, and water-holding capacity of samples was evaluated. Changes in surface meat color (L*, a*, and b* values), pH, ammonia content, and thiobarbituric acid reactive substances (TBARS) values were monitored in pork loins during 12 days of refrigerated storage. Sensory evaluation was also conducted. There was a significant decrease in pH, moisture content, and water-holding capacity of raw meat due to fruit juice marination, resulting in marination losses and higher cooking losses compared with the control and brine-marinated samples. During cold storage, marination with sour cherry and plum juices was found to slow down the increase in TBARS values and ammonia content and the decrease in all meat sensory scores. Thus, sour cherry and plum juices may be used as marinating ingredients as they promote interesting sensory properties and improve the storage stability of pork loin.
Rapid Determination of Mixed Pesticide Residues on Apple Surfaces by Surface-Enhanced Raman Spectroscopy
Authors: Wang Luyao , Ma Pei , Chen Hui , Chang Min , Lu Ping , Chen Ning , Yuan Yanbing , Chen Nan , Zhang Xuedian
Published: April 2022

Chlorpyrifos (CPF) and 2,4-dichlorophenoxyacetic acid (2,4-D) are insecticides and herbicides which has been widely used on farms. However, CPF and 2,4-D residues on corps can bring high risks to human health. Accurate detection of pesticide residues is important for controlling health risks caused by CPF and 2,4-D. Therefore, we developed a fast, sensitive, economical, and lossless surface-enhanced Raman spectroscopy (SERS)-based method for pesticide detection. It can rapidly and simultaneously determine the CPF and 2,4-D mixed pesticide residues on an apple surface at a minimum of 0.001 mg L−1 concentration, which is far below the pesticide residue standard in China and the EU. The limits of detection reach down to 1.28 × 10−9 mol L−1 for CPF and 2.47 × 10−10 mol L−1 for 2,4-D. The limits of quantification are 4.27 × 10−9 mol L−1 and 8.23 × 10−10 mol L−1 for CPF and 2,4-D. This method has a great potential for the accurate detection of pesticide residues, and may be applied to other fields of agricultural products and food industry.
Genetic Polymorphisms of IGF1 and IGF1R Genes and Their Effects on Growth Traits in Hulun Buir Sheep
Authors: Ding Ning , Tian Dehong , Li Xue , Zhang Zhichao , Tian Fei , Liu Sijia , Han Buying , Liu Dehui , Zhao Kai
Published: April 2022

The identification of candidate genes and genetic variations associated with growth traits is important for sheep breeding. Insulin like growth factor 1 (IGF1) and insulin like growth factor 1 receptor (IGF1R) are well-accepted candidate genes that affect animal growth and development. The current study attempted to assess the association between IGF1 and IGF1R genetic polymorphisms and growth traits in Hulun Buir sheep. To achieve this goal, we first identified three and ten single nucleotide polymorphisms (SNPs) in exons of IGF1 and IGF1R in Hulun Buir sheep and then constructed six haplotypes of IGF1R based on linkage disequilibrium, respectively. Association studies were performed between SNPs and haplotypes of IGF1 and IGF1R with twelve growth traits in a population encompassing 229 Hulun Buir sheep using a general linear model. Our result indicated three SNPs in IGF1 were significantly associated with four growth traits (p < 0.05). In IGF1R, three SNPs and two haplotype blocks were significantly associated with twelve growth traits (p < 0.05). The combined haplotype H5H5 and H5H6 in IGF1R showed the strong association with 12 superior growth traits in Hulun Buir sheep (p < 0.05). In conclusion, we identified SNPs and haplotype combinations associated with the growth traits, which provided genetic resources for marker-assisted selection (MAS) in Hulun Buir sheep breeding.
Impact of Groundwater Table Fluctuation on Stability of Jointed Rock Slopes and Landslides
Authors: Beyabanaki S. Amir Reza
Published: April 2022

Groundwater level plays an important role in triggering landslides. In this paper, Distinct Element Method is used to investigate the impact of groundwater table fluctuation on the stability of jointed rock slopes. For this purpose, 110 cases including different number of joint sets, joint friction angles, joint spacings, and joint angles are considered and the influence of changing groundwater level on the stability of a jointed rock slope is investigated through a series of parametric studies. This study shows that the factor of safety for slopes can decrease significantly with increasing the groundwater level, and the impact is more significant on slopes with steeper joints. Furthermore, as the spacing of the joints decreases, the impact decreases. However, as the joint spacing increases, the groundwater table should rise to a higher elevation to be able to have an impact. Moreover, the impact on the factor of safety is similar for different joint friction angles when the groundwater level elevation is high. This study provides a better understanding of the impact of groundwater table fluctuation on the stability of jointed rock slopes.
Application of the IDEAS Framework in Adapting a Web-Based Physical Activity Intervention for Young Adult College Students
Authors: Hartson Kimberly R. , Della Lindsay J. , King Kristi M. , Liu Sam , Newquist Paige N. , Rhodes Ryan E.
Published: April 2022

User-centered developmental processes are critical to ensuring acceptability of e-health behavioral interventions, and yet physical activity research continues to be inundated with top-down developmental approaches. The IDEAS (Integrate, Design, Assess, and Share) framework outlines a user-centered process for development of e-health interventions. The purpose of this manuscript is to describe the application of the IDEAS framework in adapting a web-based physical activity intervention for young adult college students. Steps 1–3 emphasized integrating insights from users and theory and Steps 4–7 focused on iterative and rapid design with user feedback. Data were collected via repeat qualitative interviews with young adult college students (N = 7). Resulting qualitative metathemes were engagement, accountability, and cultural fit. Therefore, intervention modifications focused on strategies to foster ongoing engagement with the program (e.g., increase interactivity), support personal and social accountability (e.g., private social media group), and provide a cultural fit within the college lifestyle (e.g., images relevant to student life). The resulting web-based intervention included eight weekly lessons, an expanded resource library, “how-to” videos, step and goal trackers, and a private social media group to be led by a wellness coach. In conclusion, the IDEAS framework guided an efficient, user-centered adaptation process that integrated empirical evidence and behavior change theory with user preferences and feedback. Furthermore, the process allowed us to address barriers to acceptability during the design and build stages rather than at later stages of pilot and efficacy testing.
Chronic Lumbar Pain and Insomnia in College-Aged Students
Authors: Hamaoka Katsumi , Ashizawa Ryouta , Hida Mitsumasa , Suganuma Ippei , Yoshimoto Yoshinobu
Published: April 2022

Insomnia in college students has a significant impact on academic performance and mental health (e.g., depression). Although the mechanisms underlying insomnia and chronic pain are becoming clearer, only a few studies on college students have examined these factors by their location in the body. The purpose of the present study was to identify the location of chronic pain in the body most associated with insomnia in college students. A web-based survey was used to collect information pertaining to nine questions from 494 university students: sex, age, presence of chronic pain, intensity of chronic pain, location of chronic pain, and duration of chronic pain, as well as scores from the Athens Insomnia Scale (AIS), Pain Catastrophizing Scale, and Hospital Anxiety and Depression Scale. To examine the association between insomnia and the site of chronic pain, stepwise logistic regression analysis was conducted with AIS as the target variable. The results showed a significant positive correlation between chronic pain in the lumbar region and AIS scores. Future longitudinal studies including multiple factors are necessary to clarify the causal relationship between insomnia and chronic lower back pain.
The Association between Physical Activity and Smartphone Addiction in Korean Adolescents: The 16th Korea Youth Risk Behavior Web-Based Survey, 2020
Authors: Kim Jooyoung , Lee Kihyuk
Published: April 2022

Many side effects of smartphone addiction have been reported, such as a lack of sleep, obesity, and poor concentration. However, the relationship between physical activity (PA) and smartphone addiction has not been fully elucidated. This study aimed to analyze the relationship between physical activity and smartphone addiction among 53,534 Korean adolescents using raw data from the 16th (2020) Korea Youth Risk Behavior Web-based Survey (KYRBS). The dependent variables were the general user group, potential risk user group, and high-risk user group for smartphone addiction. The independent variables were moderate PA (over 5 days per week), vigorous PA (over 3 days per week), and strength exercise (over 3 days per week). Sex, body mass index (BMI), school grade, academic achievement, sleep satisfaction, depression, loneliness, and stress were selected as confounding variables. A complex sample logistic regression analysis was performed. Potential smartphone addiction risk users showed statistically significant odds ratios of 1.423 (p < 0.001), 1.379 (p < 0.001), and 1.383 (p < 0.001) based on general users participating in moderate PA, vigorous PA, and strength exercise, respectively. High-risk users showed statistically significant odds ratios of 1.475 (p < 0.001), 1.484 (p < 0.001), and 1.619 (p < 0.001), respectively. In conclusion, to prevent smartphone addiction among Korean adolescents, participation in moderate PA for more than five days a week, vigorous PA for more than three days a week, or strength exercise for more than three days a week is considered effective.
Embryonic Development in Relation to Maternal Obesity Does Not Affect Pregnancy Outcomes in FET Cycles
Authors: Fawarseh Adham , Atzmon Yuval , Aslih Nardin , Bilgory Asaf , Shalom-Paz Einat
Published: April 2022

This retrospective cohort study examined the effect of maternal BMI on embryo morphokinetics using a time-lapse incubator (TLI) and evaluated the effect on outcomes of frozen embryo transfer (FET) cycles. The study included 641 women who underwent FET of a total of 2553 embryos from January 2017 to August 2019. The women were divided into four groups according to BMI: underweight (<18.5 kg/m2), normal weight (18.5–24.99 kg/m2), overweight (25.0–29.99 kg/m2), and obese (≥30 kg/m2). Embryos were transferred on day 3 or 5, and their development was monitored using a TLI. We found that oocytes from obese patients were slower in the extraction of the second polar body (tPB2) after fertilization and the two pronucleus stage appeared later compared to normal-weight women. The time to fading of the pronucleus (tPNf), t2, and t4 were comparable between the four groups. Oocytes from underweight and overweight women had significantly faster cleavage at t3 and t5–t8 compared to normal weight. We did not find any significant difference in pregnancy rate, clinical pregnancy rate, or miscarriage rate among groups. In conclusion, embryos from normal-weight patients had slower cleavage rates compared to obese patients, while embryo quality was similar between BMI groups. The cycle outcomes demonstrated comparable pregnancy rates among the BMI groups.
Efficacy of Four-Channel Functional Electrical Stimulation on Moderate Arm Paresis in Subacute Stroke Patients—Results from a Randomized Controlled Trial
Authors: Schick Thomas , Kolm Daniela , Leitner Andreas , Schober Sandra , Steinmetz Maria , Fheodoroff Klemens
Published: April 2022

This preliminary randomized clinical trial explores the efficacy of task-oriented electromyography (EMG)-triggered multichannel functional electrical stimulation (EMG-MES) compared to single-channel cyclic neuromuscular electrical stimulation (cNMES) on regaining control of voluntary movements (CVM) and the ability to execute arm-hand-activities in subacute stroke patients with moderate arm paresis. Twelve ischemic stroke patients (Fugl-Meyer Assessment Arm Section (FMA-AS) score: 19–47) with comparable demographics were block-randomized to receive 15 sessions of cNMES or EMG-MES over three weeks additionally to a conventional neurorehabilitation program including task-oriented arm training. FMA-AS, Box-and-Block Test (BBT), and Stroke-Impact-Scale (SIS) were recorded at baseline and follow-up. All participants demonstrated significant improvement in FMA-AS and BBT. Participants treated with EMG-MES had a higher mean gain in FMA-AS than those treated with cNMES. In the SIS daily activities domain, both groups improved non-significantly; participants in the EMG-MES group had higher improvement in arm-hand use and stroke recovery. EMG-MES treatment demonstrated a higher gain of CVM and self-reported daily activities, arm-hand use, and stroke recovery compared to cNMES treatment of the wrist only. The protocol of this proof-of-concept study seems robust enough to be used in a larger trial to confirm these preliminary findings.
Influence of Oral Dipping Tombak Smokeless Tobacco on Coagulation Profile and Platelet Counts
Authors: Elkhalifa Ahmed M. E. , Ali Nada Y. , Tamomh Abdelhakam G. , Tabash Mohammed I. , Mustafa Esraa T. A. , Mohammed Zenieb A. K. , Ahamed Nedal A. S.
Published: April 2022

The goal of this paper is to investigate the influence of oral dipping of Tombak Smokeless Tobacco (SLT) on prothrombin time (PT), activated partial thromboplastin time (APTT), international normalized ratio(INR) values, and platelet counts (PLTs), in Sudanese Tombak users. An analytical cross-sectional study was conducted at Kosti health insurance hospital, Sudan, in 2019. According to the inclusion and exclusion criteria, 100 adult users of oral Tombak for three or more years were chosen randomly as a study group. Another 100 matched healthy individuals who never used Tombak were randomly selected as a comparative group. Venous blood specimens were collected in ethylene diamine tetra-acetic acid (EDTA) containers for the PLT counts using the automated haematology analyser (Sysmex, Tokyo, Japan XK-21SYSMEX) and in trisodium citrate anti-coagulant containers for coagulation tests using a co-agulometer machine analyser. Our findings show a significant decrease in PLT count mean values in the Tombak users group (212.1 × 103/mm3 ± 74.3 × 103/mm3) compared with the non-taking Tombak group mean values (243.2 × 103/mm3 ± 83.0 × 103/mm3), (p < 0.006). Both PT and APTT were significantly prolonged in Tombak users (16.03 ± 1.22 s vs. 14.44 ± 0.557 s), p < 0.001 for PT, and (41.62 ± 7.28 s vs. 34.99 ± 4.02 s), (p < 0.001) for APTT. INR mean values were significantly longer in Tombak users (1.11 ± 0.096) vs. (1.07 ± 0.66; p < 0.001). Multiple linear regression analysis findings show a significant impact of the four investigated variables, including duration of taking Tombak, age, and frequency of taking Tombak per day (p < 0.001). In conclusion, using Tombak a Smokeless Tobacco (SLT) for a long period significantly affect Platelet counts and coagulation profile.
Growth, Fruit Yield, and Bioactive Compounds of Cherry Tomato in Response to Specific White-Based Full-Spectrum Supplemental LED Lighting
Authors: Nguyen Thi Kim Loan , Cho Kye Man , Lee Hee-Yul , Sim Han-Sol , Kim Jin-Ha , Son Ki-Ho
Published: April 2022

Supplemental artificial light in greenhouses is fundamental to achieving sustainable crop production with high yield and quality. This study’s purpose was to investigate the efficacy of supplemental light (SL) sources on the vegetative and reproductive growth of cherry tomatoes. Four types of light sources were applied, including high-pressure sodium lamps (HPS), a narrow-spectrum LED light (NSL), and two specific full-spectrum LED lights (SFL1 and SFL2) with a shorter blue peak wavelength (436 nm) and/or green peak wavelength (526 nm). The control was the natural light condition. Shoot fresh and dry weight and leaf area in the SFL1 and SFL2 treatments were greater than those in the control. The HPS and NSL treatments also enhanced tomato growth, but they were less efficient compared to the SFL treatments. The SFL1 and SFL2 treatments showed higher fruit yields by 73.1% and 70.7%, respectively, than the control. The SL sources did not affect the effective photochemical quantum yield of photosystem II (Y (II)). However, they did trigger the increased electron transport rate (ETR) and non-photochemical quenching (NPQ). The SFL treatments enhanced tomato growth, fruit yield, and efficient use of light and energy, suggesting that the specific full spectrum based on the short-wavelength blue and/or green peak can be successfully applied for the cultivation of cherry tomato and other crops in greenhouses.
First Flush Stormwater Runoff in Urban Catchments: A Bibliometric and Comprehensive Review
Authors: Maniquiz-Redillas Marla , Robles Miguel Enrico , Cruz Gil , Reyes Nash Jett , Kim Lee-Hyung
Published: April 2022

First flush is a phenomenon in stormwater runoff that has been considered a topic of great interest in the field of nonpoint source pollution. Despite several attempts to define the first flush quantitively, the specified characteristics of the phenomenon vary among sources. To address these uncertainties, a bibliometric and comprehensive review on published articles related to first flush was conducted. A corpus of 403 research articles was obtained from the Scopus database, which was then parsed using the CorText Manager for the bibliometric analysis. The study examined quantitative definitions of first flush from various sources; climate and topographic characteristics of monitoring and experimental sites where the studies on first flush were performed; the sample collection methods applied; the first flush values obtained on the studies and how it influenced the nonpoint source pollution in urban watersheds. A network map, two contingency matrices, and a Sankey diagram were created to visualize the relationship of significant keywords related to first flush, as well as their co-occurrences with journals, countries, and years. It was found that the strength of the first flush effect could vary depending on the geographical location of the site, climatic conditions, and the pollutants being analyzed. Therefore, initial rainfall monitoring, runoff sampling, and water quality testing were seen as critical steps in characterizing the first flush in urban catchments. Furthermore, the characterization of first flush was found to be significant to the selection of best management practices and design of low-impact development (LID) technologies for stormwater runoff management and nonpoint source pollution control.
Patterns of Living Lost? Measuring Community Participation and Other Influences on the Health of Older Migrants in China
Authors: Zhang Xiangjing , Zhou Wusi , Jiang Biya
Published: April 2022

Community participation is a key element of active aging that promotes a new paradigm to enhance health and well-being as people age. However, social isolation is often a concern for older migrants. In this study, we aimed to investigate the current status of older migrants’ community participation and assess the main influences on three forms of welfare, development, and organizational participation. We adopted a quantitative research design for this study. A questionnaire survey was completed by 1216 older migrants in 4 cities; 1105 valid responses were received, representing a response rate of around 91%. The research findings showed that the current participation of older migrants in community activities was limited. By comparison, full self-care capability and non-chronic illness positively affected general and welfare participation. Educated at primary school had a negative influence on general, development, and organizational participation, whereas knowledge of the local language was a significant predictor of general and development participation. Urban inclusion and resident friendship had positive effects on general, welfare, and development participation. The study also revealed direct influences of socioeconomic characteristics on different types of participation. Moving forward, actions are needed to maximize older migrants’ participation in public events and community life.
On the Road to Camarón: The Sleep of an Ultra-Endurance Athlete Cycling 10,000 km in 24 Days
Authors: Nédélec Mathieu , Chauvineau Maxime , Guilhem Gaël
Published: April 2022

The impact of sleep on performance is fundamental for ultra-endurance athletes, but studies on this issue are rare. The current investigation examined the sleep and performance of a cyclist engaged in a simulated 10,000 km tour. The sleep behavior of the athlete (age, 57; height, 1.85 m; mass, 81 kg) before, during (i.e., 23 nights), and after the tour was monitored using a reduced-montage dry-electroencephalographic (EEG) device. The daily performance (i.e., number of kms) was recorded throughout the race. The cyclist set a new world record, completing 10,358 km in 24 days with a mean daily distance of ≈432 km in approximately 16 h, i.e., an average speed of ≈27 km/h. Sleep duration throughout the tour (5:13 ± 0:30) was reduced compared to the baseline sleep duration (7:00 ± 1:00), with a very large difference (ES = 2.3). The proportion of N3 during the tour (46 ± 7%) was compared to the measured N3 proportion during the baseline (27 ± 5%) and was found to be systematically outside the intra-individual variability (mean ± 1 SD), with a very large difference (ES = 3.1). This ultra-endurance event had a major influence on sleep-duration reduction and a notable modification in sleep architecture. The increase in slow-wave sleep during the race may be linked to the role of slow-wave sleep in physiological recovery.
Assessing Burden, Anxiety, Depression, and Quality of Life among Caregivers of Hemodialysis Patients in Indonesia: A Cross-Sectional Study
Authors: Pio Theresia Maria Toji , Prihanto Junaidi Budi , Jahan Yasmin , Hirose Naoki , Kazawa Kana , Moriyama Michiko
Published: April 2022

Family caregivers endure the burden of caring for patients receiving hemodialysis, which can affect their psychological status and may disrupt the care process. This study aims to assess the level of burden, anxiety, depression, and quality of life (QOL) among family caregivers, to investigate the influence of caregivers’ sociodemographic factors and patients’ clinical conditions on the level of burden, and investigate how burden affects anxiety/depression and QOL. A descriptive, cross-sectional study was conducted from September to October 2020. A total of 104 caregivers with a mean age of 44.4 ± 12.7 years (63.5% women) in the hemodialysis department of a hospital in Indonesia were examined. Zarit Burden Interview (ZBI), Hospital Anxiety and Depression Scale, and WHOQOL-BREF were used. Descriptive analysis was conducted to assess the level of psychological status, and multiple regression analysis and path analysis were performed to evaluate the association among all factors. As result, regarding burden, 10.2% had a moderate-to-severe burden, and in terms of anxiety and depression, 25% and 9.6% showed abnormal cases; the mean scores of 4 domains of QOL were about 60 points. Burden and anxiety/depression were significantly negatively associated with all domains of QOL (p < 0.001); however, sociodemographic and clinical factors were not associated with any of the QOL domains. The path analysis results showed that burden positively correlated with anxiety/depression and negatively correlated with QOL via anxiety/depression. In conclusion, family caregivers’ QOL was found to be indirectly influenced by burden through anxiety/depression. Further evaluation of clinical significance and implications for caregiver’s lifestyle regarding anxiety and depression, which result in caregiver burden, is warranted.
Why Don’t You [Government] Help Us Make Healthier Foods More Affordable Instead of Bombarding Us with Labels? Maternal Knowledge, Perceptions, and Practices after Full Implementation of the Chilean Food Labelling Law
Authors: Correa Teresa , Fierro Camila , Reyes Marcela , Taillie Lindsey Smith , Carpentier Francesca Renee Dillman , Corvalán Camila
Published: April 2022

Experimental and real-life evaluations show that the use of front-of-package warning labels (FoP) in unhealthy foods is well understood and can modify people’s behaviors. However, it is unclear whether these effects remain in the long term because of the risk of message fatigue. The purpose of this study is to explore after four years of implementation of the Chilean Food Labelling law people’s dietary behavior and FoP labels attention. Nine focus groups of mothers (7–10 people each) of children (2–14 yo) were conducted in Santiago, Chile, and macrocodes were developed, combining an iterative process of deductive and inductive thematic analyses. We found that mothers experienced labels’ fatigue but also had greater knowledge about nutrition and appreciation for more natural foods. This greater knowledge about better nutrition interferes with the perception that healthier and less processed foods are financial and physically inaccessible. The key role of schools as an environment for promoting healthier diets in children was strengthened by the mothers. These results suggest that policies based on providing consumer information need reinforcement campaigns to maintain their effectiveness and that we also need to advance policies to improve access and affordability of healthy foods to ensure better diets.
Training Computers to See the Built Environment Related to Physical Activity: Detection of Microscale Walkability Features Using Computer Vision
Authors: Adams Marc A. , Phillips Christine B. , Patel Akshar , Middel Ariane
Published: April 2022

The study purpose was to train and validate a deep learning approach to detect microscale streetscape features related to pedestrian physical activity. This work innovates by combining computer vision techniques with Google Street View (GSV) images to overcome impediments to conducting audits (e.g., time, safety, and expert labor cost). The EfficientNETB5 architecture was used to build deep learning models for eight microscale features guided by the Microscale Audit of Pedestrian Streetscapes Mini tool: sidewalks, sidewalk buffers, curb cuts, zebra and line crosswalks, walk signals, bike symbols, and streetlights. We used a train–correct loop, whereby images were trained on a training dataset, evaluated using a separate validation dataset, and trained further until acceptable performance metrics were achieved. Further, we used trained models to audit participant (N = 512) neighborhoods in the WalkIT Arizona trial. Correlations were explored between microscale features and GIS-measured and participant-reported neighborhood macroscale walkability. Classifier precision, recall, and overall accuracy were all over >84%. Total microscale was associated with overall macroscale walkability (r = 0.30, p < 0.001). Positive associations were found between model-detected and self-reported sidewalks (r = 0.41, p < 0.001) and sidewalk buffers (r = 0.26, p < 0.001). The computer vision model results suggest an alternative to trained human raters, allowing for audits of hundreds or thousands of neighborhoods for population surveillance or hypothesis testing.
Tips for Managing Ethical Challenges in Advance Care Planning: A Qualitative Analysis of Japanese Practical Textbooks for Clinicians
Authors: Hirakawa Yoshihisa , Aita Kaoruko , Nishikawa Mitsunori , Arai Hidenori , Miura Hisayuki
Published: April 2022

(1) Background: While advance care planning (ACP) provides healthcare professionals with valuable tools to meet patients’ needs in a person-centered manner, several potential ethical challenges are inherent to the process. However, recent studies have largely focused on ACP practicalities such as implementation, execution, and completion rather than on the ethical challenges that clinicians routinely encounter in ACP practices. (2) Research question/aim/objectives: This study aimed to identify tips for clinicians managing ethical challenges in ACP practices. (3) Methods: It performed a brief search for all Japanese published books pertaining to ACP practice available as of January 2021 using the keywords “advance care planning (ACP)” and “autonomy” and analyze the content of nine practical ACP textbooks for clinicians. (4) Results: Two major themes capturing the essential recommendations for managing ethical challenges in ACP were ultimately identified, namely interprofessional ethics and informed consent. (5) Conclusion: The findings suggested tips for managing ethical challenges in ACP: refer to ethical frameworks for interprofessional collaboration and ethical decision making, assess decision-making capacity of family substitute decision makers and one’s eligibility for the role, understand the standard process of informed consent and how to handle situations when the patient are not well informed about the diagnosis and prognosis of non-cancer illness.
Relationship between Health-Related Physical Fitness Parameters and Functional Movement Screening Scores Acquired from a Three-Dimensional Markerless Motion Capture System
Authors: Cabarkapa Dimitrije , Whetstone Joseph M. , Patterson Aaron M. , Mosier Eric M. , Cabarkapa Damjana V. , Fry Andrew C.
Published: April 2022

The purpose of the present study was to examine the relationship between five algorithm-derived functional movement screening scores (i.e., readiness, explosiveness, functionality, dysfunction, and vulnerability) obtained from an innovative three-dimensional markerless motion capture system (3D-MCS) and some of the key health-related physical fitness parameters such as maximal aerobic capacity (VO2max), body mass index (BMI), body fat percentage (BF%), waist and hip circumferences (WC and HC), and high-density lipoprotein cholesterol (HDL-C). BF% showed a weak positive correlation with vulnerability and moderate-to-strong negative correlations with readiness, explosiveness, and functionality scores. Similarly, but opposite to BF%, VO2max showed a weak negative correlation with vulnerability and moderate-to-strong positive correlations with readiness, explosiveness, and functionality scores. BMI, WC, and HC showed moderate negative correlations with vulnerability, readiness, and functionality scores, while HDL-C showed a weak positive correlation with vulnerability and a weak negative correlation with explosiveness scores. Therefore, it appears that 3D-MCS may be used a as a non-invasive testing alternative or in conjunction with currently implemented traditional testing modalities to provide health practitioners with additional information regarding some of the key health-related physical fitness parameters, especially within non-academic environments such as wellness and clinical settings.
The Gambling Habits of University Students in Aragon, Spain: A Cross-Sectional Study
Authors: López-del-Hoyo Yolanda , Monreal-Bartolomé Alicia , Aisa Pablo , Pérez-Aranda Adrián , Plana Carlos , Poblador José Antonio , Casterad Jaime , García-Campayo Javier , Montero-Marin Jesus
Published: April 2022

Gambling has become a routine form of entertainment for many young people. The aim of this study was to describe the gambling behavior that university students are developing in Aragon, Spain, and to analyze whether these habits are more common among students of sports science, on the assumption that they are more likely to have a higher exposure to betting company marketing. A cross-sectional design was applied, with data collected on advertising exposure, gambling habits and experiences, and opinions on the impact of gambling and its regulation from 516 undergraduate students from the University of Zaragoza. The online survey included ad hoc questions and the “Pathological Gambling Short Questionnaire” to screen for potential gambling disorders. Almost half of the sample had bet money at least once in their life (48.1%), and 2.4% screened positive for consideration of a possible diagnosis of pathological gambling. Betting shops (44.2%) were the most common gambling option, and students of sports science showed a higher prevalence of pathological gambling and had greater tendencies to make bets. Gambling is perceived as a normal leisure activity by a significant part of university students. The development of transversal strategies is required to raise awareness towards the potential dangers of gambling.
Occurrence and Removal of Triazine Herbicides during Wastewater Treatment Processes and Their Environmental Impact on Aquatic Life
Authors: Wang Meng , Lv Jiapei , Deng Haowei , Liu Qiong , Liang Shuxuan
Published: April 2022

Wastewater treatment plants (WWTPs) represent a major point source for pesticide residue entry to aquatic environment and may threaten ecosystems and biodiversity in urban area. Triazine herbicides should be paid attention to for their ubiquitous occurrence in the environment and long-term residue. The present study aimed to quantify eleven compounds of triazine herbicides during wastewater treatment processes. The solid phase extraction and gas-chromatography mass spectrometry (GC-MS) determination method were developed to identify the target herbicides with approving sensitivity. The pollution levels, removal rates of eleven triazine herbicides along five different treatment stages in WWTP were investigated. The results showed that three herbicides including atrazine, simetryn and prometryn were detected. Their concentrations in influent were among 28.79 to 104.60 ng/L. Their total removal rates from influent to effluent were 14.92%, 10.79% and 4.41%, respectively indicating that they were difficult to be effectively remove during wastewater treatment. Regarding the negative impact of triazine herbicides discharged from WWTPs on downstream water quality and aquatic life, the environmental risks were assessed by calculating the Environmental Relevance of Pesticides from Wastewater Treatment Plants Index (ERPWI) and water cycle spreading index (WCSI). The risk assessment results denoted the possible high risks for atrazine and simetryn to alage, and simetryn concurrently posed a high risk for the daphnia, while prometryn was at medium risk to alage. Atrazine and simetryn in effluent posed high risk for algae, meanwhile, simetryn had high risk for Daphnia. These results suggested a possible threat to the aquatic environment, rendering in this way the ERPWI method as a useful assessment tool. Further extensive study is needed for atrazine and simetryn in order to better understand their migration mechanism in aquatic environment.
Acute Exercise with Moderate Hypoxia Reduces Arterial Oxygen Saturation and Cerebral Oxygenation without Affecting Hemodynamics in Physically Active Males
Authors: Mulliri Gabriele , Magnani Sara , Roberto Silvana , Ghiani Giovanna , Sechi Fabio , Fanni Massimo , Marini Elisabetta , Stagi Silvia , Lai Ylenia , Rinaldi Andrea , Isola Raffaella , Vargiu Romina , Spranger Marty D. , Crisafulli Antonio
Published: April 2022

Hemodynamic changes during exercise in acute hypoxia (AH) have not been completely elucidated. The present study aimed to investigate hemodynamics during an acute bout of mild, dynamic exercise during moderate normobaric AH. Twenty-two physically active, healthy males (average age; range 23–40 years) completed a cardiopulmonary test on a cycle ergometer to determine their maximum workload (Wmax). On separate days, participants performed two randomly assigned exercise tests (three minutes pedaling at 30% of Wmax): (1) during normoxia (NORMO), and (2) during normobaric AH at 13.5% inspired oxygen (HYPO). Hemodynamics were assessed with impedance cardiography, and peripheral arterial oxygen saturation (SatO2) and cerebral oxygenation (Cox) were measured by near-infrared spectroscopy. Hemodynamic responses (heart rate, stroke volume, cardiac output, mean arterial blood pressure, ventricular emptying rate, and ventricular filling rate) were not any different between NORMO and HYPO. However, the HYPO test significantly reduced both SatO2 (96.6 ± 3.3 vs. 83.0 ± 4.5%) and Cox (71.0 ± 6.6 vs. 62.8 ± 7.4 A.U.) when compared to the NORMO test. We conclude that an acute bout of mild exercise during acute moderate normobaric hypoxia does not induce significant changes in hemodynamics, although it can cause significant reductions in SatO2 and Cox.
Evaluation of Knowledge, Attitudes and Practices Related to Self-Testing Procedure against COVID-19 among Greek Students: A Pilot Study
Authors: Marinos Georgios , Lamprinos Dimitrios , Georgakopoulos Panagiotis , Oikonomou Evangelos , Zoumpoulis Georgios , Garmpis Nikolaos , Garmpi Anna , Tzalavara Eirini , Siasos Gerasimos , Rachiotis Georgios , Papaioannou Anastasia , Schizas Dimitrios , Damaskos Christos
Published: April 2022

The COVID-19 pandemic has had a major impact on health, economy, society and education. In the effort to return to normalcy, according to the instructions of the Greek Government for the resumption of the operation of schools, a screening Rapid Antigen Detection Test with the method of self-testing is required for students twice per week, for the early identification and isolation of positive cases. We aimed to pivotally investigate the knowledge, attitudes and practices related to self-testing procedures against COVID-19 among Greek students. A questionnaire was distributed to a convenient sample of students in the region of Athens. Information about the vaccination coverage against SARS-CoV-2 was also obtained. Our study included 1000 students, with 70% of them having an average grade at school. Most of the participants were aware of coronavirus (98.6%) and the self-test (95.5%). The vast majority of students (97%) performed self-testing twice per week, with the 70% them being assisted by someone else. Nearly one sixth of the participants had been infected by COVID-19 (14%) while 36% of them have already been vaccinated against SARS-CoV-2. In conclusion, we report high compliance with the COVID-19 self-testing procedure among students in Attica, Greece. Older age adolescents are more likely to not comply with the regulations of self-testing. Consequently, tailored interventions targeted at older age adolescents are warranted in order to increase the acceptability of self-testing.