bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetics of Vascular Inflammation in Lower Limb Ischemia: The TNF-α Hypothesis in Peripheral Arterial Disease
Authors: Caicedo Diego , Álvarez Clara V. , Pérez Sihara , Devesa Jesús
Published: December 2021

Background: Vascular inflammation plays a crucial role in peripheral arterial disease (PAD), although the role of the mediators involved has not yet been properly defined. The aim of this work is to investigate gene expression and plasma biomarkers in chronic limb-threating ischemia (CLTI). Methods: Using patients from the GHAS trial, both blood and ischemic muscle samples were obtained to analyze plasma markers and mRNA expression, respectively. Statistical analy-sis was performed by using univariate (Spearman, t-Student, X2) and multivariate (multiple lo-gistic regression) tests. Results: 35 patients were available at baseline (29 for mRNA expression). Baseline characteristics (mean): Age:71.4±12.4 (79.4% male); TNF-α:10.7±4.9; hs-CRP:1.6±2.2; Neutrophil-to-lymphocyte ratio (NLR):3.5±2.8. Plasma TNF-α was found elevated (≥8.1) in 68.6% of patients, while high hs-CRP (≥0.5) in 60.5%. Diabetic patients with high level of inflammation showed significantly higher levels of NOX4 expression at baseline (p=0.0346). Plasma TNF-α had a negative correlation with eNOS expression (-0.5, p=0.015) and hs-CRP with VEGF-A (-0.63, p=0.005). The expression of NOX4 was parallel to that of plasma TNF-α (0.305, p=0.037), especial-ly in DM. Cumulative mortality at 12-month was related to NLR ≥3 (p=0.019) and TNF-α ≥8.1 (p=0.048). The best cut-off point for NLR to predict mortality was 3.4. Conclusions: NOX4 and TNF-α are crucial for the development and complications of lower limb ischemia, especially in DM. hs-CRP could have a negative influence on angiogenesis too. NLR and TNF-α represent suita-ble markers of mortality in CLTI. These results are novel because they connect muscle gene expres-sion and plasma information in patients with advanced PAD, deepening the search of new and ac-curate targets for this condition.
Hydro-Damage Properties of Red-Bed Mudstone Failures Induced by Nonlinear Seepage and Diffusion Effect
Authors: Ye Zhenni , Liu Xiaoli , Wang Enzhi , Sun Huan , Dong Qinxi
Published: December 2021

Nonlinear catastrophes caused by geological fluids are a fundamental issue in rock mechanics and the geoengineering hazard field. For the consideration of hydrodynamic force on red-bed mudstone softening damage, X-ray visualization test on the fissure flow in mudstone block failure under hydrodynamic force was performed in this study based on block scale and the physical phenomena of fissure seepage and nonlinear diffusion were further explored. A new method for evaluating the hydro-damage degrees of rocks using an X-ray image analysis was proposed, and the quantitative relation of diffusion coefficients of hydro-damage and seepage was established. The research results revealed that the hydrodynamic force promoted the fluid-filled fissure behavior in mudstone specimen failure. Also, the seepage and diffusion phenomena of fluid in rocks during failures were indicated using X-ray imaging. A dual mechanical behavior was presented in the nonlinear seepage and abnormal diffusion of a red mudstone geological body under hydrodynamic conditions. The damaged degree of mudstone was aggravated by the effect of hydrodynamic force, and the initial seepage–diffusion coefficient with respect to lower hydro-damage was larger than the final seepage–diffusion coefficient with respect to higher hydro-damage of rocks with a decreasing nonlinear trend.
Linear Quadratic Regulator and Fuzzy Control for Grid-Connected Photovoltaic Systems
Authors: Mukhatov Azamat , Thao Nguyen Gia Minh , Do Ton Duc
Published: December 2021

This work presents a control scheme to control the grid-connected single-phase photovoltaic (PV) system. The considered system has four 250W solar panels, a non-inverting buck-boost DC-DC converter, and DC-AC inverter with LCL filter. The control system aims to track and operate at the maximum power point (MPP) of PV panels, regulate the voltage of DC link, and supply the grid with a unity power factor. To well achieve these goals, the proposed control system consists of three parts, that are MPP tracking controller module with a fuzzy-based modified incremental conductance (INC) algorithm, a DC-link voltage regulator with a hybrid fuzzy proportional-integral (PI) controller, and a Current Controller module using the linear quadratic regulator (LQR) for grid-connected power. Based on fuzzy control and LQR, this work introduces a full control solution for grid-connected single-phase PV systems. The key novelty of this research is to analyze and prove that the newly proposed method is more successful in numerous aspects by comparing and evaluating the previous and present control methods. The designed control system settles quickly, which is critical for output stability. In addition, as compared to backstepping approach used in our past study, the LQR technique is more resistant to sudden changes and disturbances. Furthermore, backstepping method produces the larger overshoot, which has a detrimental impact on efficiency. Simulation findings under various weather conditions were compared to theoretical ones to indicate that the system can deal with variations in weather parameters.
Analisis Pemasaran Karet Sistem Lelang di Kecamatan Kuantan Mudik Kabupaten Kuantan Singingi
Authors: Kurniati Dina , Maharani Evy , Edwina Susy
Published: December 2021

Penelitian ini memiliki tujuan untuk mengetahui sistem pemasaran pada pasar lelang dan menganalisis biaya pemasaran, margin pemasaran, keuntungan pemasaran, bagian yang diterima petani dan efisiensi pemasaran pada pasar lelang karet di Kecamatan Kuantan Mudik. Metode pengambilan sampel petani secara purpusive sampling sebanyak 23 orang dan pengambilan sampel pedagang dengan menggunakan metode sensus sebanyak 8 orang. Hasil penelitian menunjukan sistem pemasaran lelang terjadi karena permasalahan yang dihadapi petani yaitu saluran pemasaran yang panjang dan pedagang bisa mengendalikan harga bokar dikarenakan mutu bokar rendah. Petani yang mengikuti pasar lelang harus memenuhi standar mutu bokar yang ditetapkan Asosiasi Petani Karet Kuantan Singingi (APKARKUSI) dan tergabung kedalam kelompok tani atau gapoktan. Petani memiliki keterbatasan dalam memasarkan bokar langsung ke pabrik, oleh sebab itu petani melibatkan lembaga pemasaran. Saluran pemasaran karet pada pasar lelang di mulai dari petani-pedagang besar-pabrik. Petani yang mengikuti pasar lelang dilakukan pemotongan volume bokar sebesar 5% yang terdiri 3% penyusutan, 1% biaya transportasi dan 1% biaya operasional kelompok. Harga jual bokar pada Bulan Juli 2020 yang diterima petani sebesar Rp7.367,50/kg, total biaya pemasaran Rp1.316,56/kg, margin pemasaran Rp1.596,25/kg, keuntungan pemasaran Rp229,69/kg, farmer’s share 82,19 % dan efisiensi pemasaran 14,69%. Sistem pemasaran lelang ini efisien digunakan petani dalam proses pemasaran bokar yang ada di Kecamatan Kuantan Mudik, dikarenakan efisiesi dibawah 50% bearti semakin rendah persentase efisiensi pemasaran maka pemasaran semakin efisien.
PEMBUATAN VIDEO MOTION GRAPHIC DATA PENERIMAAN MAHASISWA BARU UNIVERSITAS NEGERI JAKARTA TAHUN 2018
Authors: Arasy Zamanullail  , Hamidillah Ajie  , M. Ficky Duskarnaen
Published: December 2021

Penelitian ini dilakukan dengan tujuan untuk membuat suatu media informasi yang berisikan tentang informasi data penerimaan mahasiswa baru Universitas Negeri Jakarta pada tahun 2018. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan Research and Development atau penelitian dan pengembangan. Sedangkan dalam mengembangkan produk digunakan model pengembangan Luther. Penelitian ini dilakukan sejak April 2019 sampai July 2019 bertempat di Universitas Negeri Jakarta. Data mahasiswa yang digunakan dalam penelitian ini didapat dari UPT TIK Universitas Negeri Jakarta, UKT Dikti, BPA tahun 2012 dan objek penelitian ini adalah Calon mahasiswa Universitas Negeri Jakarta.
PERANCANGAN SISTEM E-LEARNING BERBASIS LMS MOODLE PADA MATA KULIAH ALGORITMA DAN PEMROGRAMAN DI PROGRAM STUDI PENDIDIKAN TEKNIK INFORMATIKA DAN KOMPUTER UNIVERSITAS NEGERI JAKARTA
Authors: Septiani Dian Lestari  , Hamidillah Ajie  , M. Ficky Duskarnaen
Published: December 2021

Perkuliahan Algoritma dan Pemrograman di Program Studi Pendidikan Teknik Informatika dan Komputer Universitas Negeri Jakarta selama ini menggunakan pembelajaran tatap muka di kelas belum memanfaatkan e-learning. Hasil yang didapat dari pembelajaran ini belum sesuai dengan harapan dosen pengampu karena banyak mahasiswa yang mengulang dan mahasiswa tidak aktif saat diskusi di kelas. Penelitian ini bertujuan untuk mengetahui bagaimana rancangan sistem e-learning berbasis LMS MOODLE pada mata kuliah Algoritma dan Pemrograman di Program Studi Pendidikan Teknik Informatika dan Komputer Universitas Negeri Jakarta. Penelitian ini menggunakan metode penelitian rekayasa teknik. Untuk pembuatan sistem e-learning berbasis LMS MOODLE menggunakan model ADDIE. Pengujian sistem e-learning yang telah dibangun dilakukan dengan Uji Ahli Media dan User Acceptance Test kepada dosen pengampu mata kuliah Algoritma dan Pemrograman dan mahasiswa Pendidikan Teknik Informatika dan Komputer 2018 yang mengambil mata kuliah tersebut. Berdasarkan hasil pengujian oleh ahli media mendapat hasil CVR sebesar 0,9 yang berada pada kriteria layak. Selanjutnya hasil pengujian User Acceptance Test dosen pengampu dari indikator menyatakan kemudahan e-learning mendapatkan nilai prosentase sebesar 91% (valid atau sesuai). Dan dari indikator yang menyatakan tampilan e-learning mendapatkan nilai prosentase sebesar 80% (valid atau sesuai). Serta hasil pengujian User Acceptance Test mahasiswa dari indikator yang menyatakan kemudahan e-learning mendapatkan nilai prosentase sebesar 82% (valid atau sesuai). Dan rata-rata indikator yang menyatakan tampilan e-learning mendapatkan nilai prosentase sebesar 73% (cukup valid atau cukup sesuai). Maka dapat disimpulkan bahwa e-learning berbasis LMS MOODLE dikategorikan “sesuai” dan memiliki kemudahan dalam penggunan serta memiliki tampilan yang user friendly.
ANALISIS SCIENTOMETRIC DAN BIBLIOMETRIC UNTUK PEMETAAN BIDANG KEILMUAN DI PROGRAM STUDI PENDIDIKAN TEKNIK INFORMATIKA DAN KOMPUTER UNJ
Authors: Fathiah  , Widodo  , Bambang Prasetya Adhi
Published: December 2021

Dalam bidang keilmuan diperlukan sebuah pemetaan yang tepat dan jelas untuk setiap bidang. Pemetaan tersebut dapat dijadikan sebagai bahan acuan dalam mepertimbangkan pengambilan keputusan yang relevan dengan bidang keilmuan yang dimiliki. Tujuan dari penelitian ini untuk melihat kecenderungan bidang keilmuan dengan cara pemetaan analisis scientometric dan bibliometric di program studi Pendidikan Teknik Informatika dan Komputer UNJ. Proses pemetaan ini dimulai dengan pengumpulan data mata kuliah, bimbingan skripsi dan artikel/makalah dosen. Kemudian, data tersebut dianalisis dan divisualisasikan dengan software VOSViewer. Hasil analisis menunjukkan kecenderungan bidang Computer Science berdasarkan mata kuliah, Information Technology berdasarkan bimbingan skripsi, dan Pendidikan berdasarkan artikel/makalah dosen.
UJI KECEPATAN RASPBERRY PI SEBAGAI PRIVATE CLOUD STORAGE UNTUK SMALL OFFICE HOME OFFICE: DENGAN STUDI KASUS DI UPT TIK
Authors: Ferdinand Louis  , M. Ficky Duskarnaen  , Hamidillah Ajie
Published: December 2021

Small Office Home office atau SOHO adalah sebutan bagi sebuah entitas bisnis berukuran kecil dengan jumlah karyawan tidak lebih dari sepuluh orang. Sebuah soho umumnya merupakan berupa bisnis rintisan yang memiliki keterbatasan dalam pendanaan untuk pengadaan media penyimpanan terpusat. Untuk mengatasi permasalahan tersebut SOHO biasanya menggunakan layanan komputasi awan yaitu public cloud storage yang dimana akan digunakan sebuah server virtual dari penyedia layanan sebagai tempat pencadangan data kantor. Namun pada layanan public cloud storage pemilik bisnis tidak mendapat jaminan secara konkret jika data yang tersimpan pada server penyedia layanan tidak dapat dilihat oleh pihak dari luar kantor. Oleh karena itu peneliti berencana membuat sebuah private cloud storage menggunakan perangkat Raspberry PI 3B+ yang berukuran ringkas sehingga mudah disimpan, dan bersifat eksklusif hanya untuk individu di dalam entitas bisnis. Purwarupa ini diharapkan dapat memenuhi kebutuhan sebuah Small Office Home Office akan penyimpanan terpusat yang handal, aman, dan praktis dalam penerapannya. Pengembangan perangkat menggunakan metode purwarupa, spesifikasi Raspberry Pi yang digunakan masuk dalam golongan low-budget server. Penelitian diawali dengan menganalisa kebutuhan SOHO, lalu dilanjutkan dengan mendesain purwarupa seperti penggunaan kustomisasi yang diperlukan perangkat dan penerapan pada kondisi riil untuk diujikan kembali. Pengujian dilakukan untuk mengukur kecepatan dan membandingkan kecepatan transfer hasil pengujian dengan estimasi kecepatan yang dibutuhkan untuk layanan cloud computing pada SOHO. Private cloud storage pada penelitian ini menunjukan hasil bahwa transfer data belum optimal memenuhi kebutuhan penyimpanan terpusat dengan 10 user dan 2 akses ISP.
Design of Visible Broadband Metamaterial Absorber Based on Metal Nickel
Authors: Wang Yunji , Liu Fei , Chen Lin
Published: December 2021

In this paper,we have proposed a visible broadband metamaterial absorber based on metal Nickel. The metamaterial absorber (MA) is composed of three layers,which are Nickel-based pattern array on the top, silicon dioxide in the middle layer and Nickel film at the bottom. In the whole visible spectrum(380-780nm) of our study, the average absorption of the absorber is up to 95.60%. The absorption rates at 500nm and 637nm are 96.63% and 99.93%, respectively. Also, the proposed MA is insensitive of polarization in the whole visible regime. Later, the mechanism of the absorption and the influence of the structural parameters on the absorption spectrum are investigated. Strong electrical and magnetic resonance are related to the absorption peak at the resonant wavelength. The results show the resonant wavelength of 500nm is fixed and the resonant wavelength of 637nm can be tuned by the parameters.The proposed structure will have important application prospects in the field of solar cells.
Super High Sensitivity Plasmonic Temperature Sensor Based on Square Ring Shape Resonator with Nanorods Defects
Authors: Pan Ji , Qianhan Shi , Ling Zheng , guanghui Wang , fang chen
Published: December 2021

A super high sensitivity plasmonic temperature sensor via a metal-insulator-metal (MIM) waveguide system is presented in this paper, the waveguide structure is composed of a square ring shape resonator with nanorods defects and a nanodisk resonator. Finite difference-time domain method (FDTD) is used to study the structure’s transmission characteristics and electromagnetic field distributions. Results show that sensitivity will be increased due to the gap plasmonic in the nanorod defect, the nanodisk resonator provides more plasmonic resonant modes for sensing. The positions and intensities of plasmonic resonant modes can be tuned by the radius of nanorod defects and coupling distance. The calculated maximum refractive index and FOM are and 3500, respectively. Compared to the structure without nanorods, the sensitivity is enhanced 33% for mode 1. For temperature sensing, the proposed structure possesses a relatively high sensitivity of about . The proposed plasmonic structure provides a basis for designing high sensitivity nano-biosensing, refractive index sensing.
From Inshore To Offshore: Microplastic Flows in Three Areas of The MSFD Italian Subregions
Authors: Sbrana Alice , Valente Tommaso , Bianchi Jessica , Franceschini Simone , Piermarini Raffaella , Saccomandi Flavia , de Lucia Andrea Giuseppe , Camedda Andrea , Matiddi Marco , Silvestri Cecilia
Published: December 2021

An increasing number of ecologists and researchers have addressed microplastics pollution in marine waters to explain the source, the transport, and the fate of these pollutants. Further data on the concentration of microplastics in the water column are crucial for understanding the impacts on ecosystems and to implement monitoring programs.This study provides information on the concentration and composition of sea water surface microplastics in three Italian subregions of the MSFD. We examined the flow of MPs from coastal to offshore areas, comparing their densities. We tested the efficiency of two sampling methodologies to evaluate the abundance and typology of MPs between marine layers. The results of this study confirm the high values of this pollutant in the Mediterranean Sea (0.029 ± 0.033 items · m-2), a MPs gradient from coastal to offshore areas, and a difference between the surface and subsurface marine layers.
Thinking magically or thinking scientifically: Cognitive and belief predictors of complementary and alternative medicine use in women with and without cancer diagnosis
Authors: Čavojová Vladimíra , Kaššaiová Zuzana , Šrol Jakub , Mikušková Eva Ballová
Published: December 2021

Background
Complementary and alternative medicine (CAM) is popular among the general population and patients with various diseases, but our understanding of the predictors of CAM use for the population of women with or without cancer diagnosis is still quite limited. This paper examines predictors of attitudes toward and use of CAM, including cognitive factors (scientific reasoning, health literacy, locus of control), beliefs (holistic and magical health beliefs pseudoscientific beliefs, and trust in doctors), sociodemographic factors, and cancer diagnosis.
Methods
The sample consisted of 177 women (103, 58.2% without cancer, 74, 41.8% with cancer diagnosis; Mage = 38.81, SD = 11.43).
Results
Pseudoscientific/magical beliefs and external locus of control were the strongest predictors of positive attitudes toward CAM and its higher use, as well as preference for CAM instead of conventional treatment. Cancer diagnosis predicted only higher CAM use, but not more positive attitudes to CAM, nor preference for CAM instead of conventional medicine. There was no difference between women with and without cancer diagnosis in using CAM after we controlled for age and education. Women in our sample had a similar level of magical beliefs, holistic health beliefs, and attitudes toward CAM regardless of their cancer diagnosis. However, women with cancer had significantly more pseudoscientific beliefs than women without cancer and a higher external locus of control over their health.
Conclusion
Women who have an inclination toward holistic and magical beliefs about health tend to favor CAM treatments independently of the cancer diagnosis, although the diagnosis of cancer also contributes to their higher use of CAM. In other words, it seems improbable that women would turn toward CAM treatment only after being diagnosed with cancer.
Optimization of Different Fitness Functions Using Reproduction, Crossover and Mutation of Genetic Algorithm With Binary Number Scheduling
Authors: CHRISTOPHER J PRINCE JEROME , LINGADURAI K , SHANKAR G
Published: December 2021

Genetic algorithms are search algorithms based on the mechanics of natural selection and natural genetics. In this paper, we investigate a novel approach to the binary coded testing process based on a genetic algorithm. This paper consists of two parts. Thefirst part addresses the problem in the traditional way of using the decimal number system to define the fitness function to study the variations of counts and the variations of probability against the fitness functions. Second, the initialpopulationsare defined using binary coded digits (genes). For the evaluation of the high fitness function values,three genetic operators, namely, reproduction, crossover and mutation, are randomly used. The results show the importance of the genetic operator, mutation, which yields the peak values for the fitness function based on binary coded numbers performed in a new way.
Fluoride Induced Leaky Gut and Bloom of Erysipelatoclostridium Ramosum Mediate the Exacerbation of Obesity in High-Fat-Diet Fed Mice
Authors: Chen Guijie , Peng Yujia , Huang Yujie , Xie Minhao , Dai Zhuqing , Cai Huimei , Dong Wei , Xu Weiqi , Xie Zhiyong , Chen Dan , Fan Xia , Zhou Wangting , Kan Xuhui , Yang Tingting , Chen Chunxu , Sun Yi , Zeng Xiaoxiong , Liu Zhonghua
Published: December 2021

Background: Fluoride, a necessary mineral element for our health, is widely presented in drinking water and foods. The intake of excessive fluoride showed potential risk to human health. A strong relation between fluoride exposure and obesity has been reported. However, the knowledge on the potential mechanisms on fluoride-induced obesity is still limited.Results: In this work, we showed here that fluoride alone did not induce obesity in normal diet fed mice, whereas, it could trigger exacerbation of obesity in high-fat diet (HFD) fed mice. Fluoride impaired intestinal barrier and activated Toll-like receptor 4 (TLR4) signaling to induce obesity, which was further verified in TLR4-/- mice. Furthermore, fluoride could deteriorate the gut microbiota in HFD mice. The fecal microbiota transplantation from fluoride-induced mice was sufficient to induce obesity, while the exacerbation of obesity by fluoride was blocked upon gut microbiota depletion. The fluoride-induced bloom of Erysipelatoclostridium ramosum belonged to Erysipelotrichaceae was responsible for exacerbation of obesity. In addition, a potential strategy for prevention of fluoride-induced obesity was proposed by intervention with polysaccharides from Fuzhuan brick tea.Conclusions: Overall, these results provide the first evidence of a comprehensive cross-talk mechanism between fluoride and obesity in HFD fed mice, which is mediated by gut microbiota and intestinal barrier. E. ramosum was identified as a crucial mediator of fluoride induced obesity, which could be explored as potential target for prevention and treatment of obesity with exciting translational value.
Anlotinib Plus Toripalimab and Nab-Paclitaxel in Patients with Locally Advanced or Metastatic Pancreatic Cancer: Study Protocol for An Open-Label, Non-Randomized, Phase II Study (ATNPA)
Authors: Chen Jingwen , Liu Yiqian , Zhu Yizhi , Cui Shiyun , Sun Chongqi , Bai Jianling , Shu Yongqian , Liu Lingxiang
Published: December 2021

Background

There have not been standard second-line or maintenance regimens with definite survival benefits so far for patients with pancreatic carcinoma who have lost the opportunity of curable resections or failed first-line chemotherapy. Anlotinib, a potent small-molecule tyrosine kinase inhibitor, exhibits anti-angiogenic and anti-tumour effects by specifically binding to multiple targets such as VEGFR, FGFR, PDGFR, c-Kit and Ret. Toripalimab, a novel anti-PD-1 mAb, has been proved to significantly prolong progression-free survival (PFS) and overall survival (OS) in various solid tumours with manageable toxicities when combining with cytotoxic chemotherapy. We design this study to assess the combination of anlotinib, toripalimab and nab-paclitaxel as a second-line or maintenance therapy for locally advanced pancreatic cancer (LAPC) or metastatic pancreatic cancer (MPC).
Patients and Methods:

This is an open-label, non-randomized, single-arm phase Ⅱ study, aimed at evaluating the efficacy and safety profile of the above-mentioned combination strategy in first-line therapy-failed LAPC or MPC. Totally 53 patients are to be enrolled and receive anlotinib (12 mg, po. qd.) plus toripalimab (240 mg, ivgtt. q3w.) and nab-paclitaxel (125 mg/m2, ivgtt, d1, d8) every 3 weeks as a cycle until disease progression or intolerable adverse events. The primary endpoint is PFS. Secondary end points include OS, disease control rate (DCR), object response rate (ORR), quality of life (QoL) and safety. Enrollment started in April 2021, and follow-up will be finished in April 2023.
Discussion and Significance:

Combination of anlotinib, toripalimab and nab-paclitaxel may promote vessel normalization and drug delivery, and activate the immune response, thus exerting synergistic anti-tumour effects and counteracting the immunosuppressive microenvironment of pancreatic cancer. As the first intending to assess this combination in pancreatic cancer, this study will provide comprehensive evidence for second-line or maintenance therapy of LAPC and MPC.
Trial registration:

ClinicalTrials.gov: ATNPA, NCT04718701. Registered January 22, 2021. (https://clinicaltrials.gov/ct2/show/NCT04718701?term=NCT04718701&draw=2&rank=1)
Genomic Analysis of a Novel Active Prophage of Hafnia Paralvei
Authors: Pan Lingting , Li Dengfeng , Lin Wei , Liu Wencai , Qin Weinan , Xu Lihua , Tong Yigang
Published: December 2021

Relatively little is known about the prophages in Enterobacterales bacteria except Escherichia coli. A novel phage, Hafnia phage yong2, was induced from Hafnia paralvei by mitomycin C. The phage has an elliptical head of approximately 45 × 38 nm, a long noncontractile tail of approximately 157 × 4 nm. The complete genome of Hafnia phage yong2 is a 39,546 bp double-stranded DNA with a G+C content of 49.9%, containing 59 opening reading frames (ORFs), and having at least one fixed terminus (GGGGCAGCGACA) with R=107>100. In phylogenetic analysis, Hafnia phage yong2 clustered with 4 predicted Hafnia prophages and 1 predicted Enterobacteriaceae prophage. The prophages cluster and Drexlerviridae family together formed two distinct subclades nested within a clade, suggesting the existence of a novel class of prophages with conserved sequences and unique evolutionary status not yet studied before in Hafniaceae and Enterobacteriaceae bacteria.
Left-Turn Driving Behavior Based On Situational Anger: Day Or Night
Authors: Xiong Jingjing , Mao Yan , Wang Xuan , He Wu
Published: December 2021

Anger is a key factor affecting drivers' subjective judgment and driving skills. The influence of anger on driving behavior has been widely studied, but there is a lack of comparative research under different lighting conditions. Through driving simulation experiment, this paper studies the influence of anger on left-turn driving behavior under two light conditions of day and night. In the experiment, 32 licensed participants were divided into two groups, one in emotional neutrality and the other in anger. Among them, the emotional state of anger is induced by a traffic related video. The results showed that compared with daytime participants, participants at night had higher anger intensity, shorter gap acceptance and post encroachment time (PET) when left-turn driving. In addition, compared with emotion neutral participants, angry participants tended to accept shorter gap acceptance and post encroachment time (PET) when turning left. This indicates that participants failed to respond correctly to left-turn driving behavior in a state of anger. However, the response of gender differences to situational driving anger was not affected by light conditions. The anger intensity of male participants during the day and night was higher than that of female participants, and the gap acceptance and post encroachment time (PET) during left-turn were shorter than that of female participants. This shows that male participants are more likely to produce high-intensity anger and are more likely to make dangerous driving decisions in a state of anger. This paper puts forward some suggestions on the identification of anger and the prevention of angry driving.
An Analysis of the Association Between Work Stress and Health Behaviours in Korean and Japanese Ageing Studies: a Cross-sectional Study
Authors: Cheng Taozhu , Guo Jing , Hynek Pikhart
Published: December 2021

Background and Objectives: Work stress is a risk factor that may be partially responsible for increased cardiovascular morbidity and mortality. One of the proposed pathways is through unhealthy behaviours. As limited research has focused on the association between work stress and health behaviours in Asian countries, this research aims to explore the effect of work stress on the two health behaviours among employees aged 45 or above in Korea and Japan.Methods: This cross-sectional study based on baseline data from the Korean Longitudinal Study of Aging (KLoSA,2006) and Japanese Study of Aging and Retirement (JSTAR,2007&2009) used logistic regression and multinomial logistic regression to investigate the association between work stress represented by the short version of the effort-reward imbalance (ERI) model and smoking (binary current smoking) and drinking (categorical volume of alcohol). In addition, the statistical power was over 80% for odds ratios (ORs) greater than 1.3 in Korea and 1.6 in Japan. Moreover, this research additionally examines the potential interaction between ERI and gender.Results: Smoking and drinking were significantly associated with the ER ratio in the Korean analysis (N=3,478). After the model was fully adjusted, the OR (95% CI) and RRR (95% CI) were 1.45 (1.17-1.80) and 1.43 (1.09-1.90), respectively. In Japan (N=1,504), smoking was associated with the ER ratio (OR 1.37 (1.00-1.87)); however, drinking was not. No statistically significant interaction was found between ERI and gender (p value of 0.82 in Korea and 0.19 in Japan).Conclusions: The results of this study showed that work stress was statistically significantly associated with both health behaviours in the Korean sample and with smoking in Japan. These results potentially suggest the integration of long working time reduction health promotion programmes in these two Asian countries.
Combined Negative Effects of Microplastics and Plasticizer DEHP: the Increased Release of Nets Delays Wound Healing
Authors: Shi Xu , Cui Wei , Xu Tong , Qi Xue , Miao Zhiruo , Xu Shiwen
Published: December 2021

Introduction: Environmental pollutants microplastics (MPs) and di (2-ethyl) hexyl phthalate (DEHP) can cause damage to multiple organs by causing oxidative stress. Oxidative stress participates in the healing of skin wounds through the release of neutrophil extranets (Nets). Here, we studied the effects of DEHP and MPs on skin wound healing in mice after single and combined exposure for 1 month. Results: The results showed that MPs delayed the healing of skin wounds, and the combination of the two delayed wound healing more significantly. The results of in vivo and in vitro experiments showed that the release of oxidative stress and Nets in the single exposure group increased, and the combined exposure group increased more. Further mechanism studies showed that the skin chemokines of the single exposure group increased, the NF-κB pathway was activated, the Wnt pathway was inhibited, and the epidermal growth factor and fibrosis-related indicators decreased. The combined exposure group showed a more obvious trend.Conclusion: In summary, the above results indicate that DEHP combined with MPs induces an increase in the release of Nets by causing excessive skin ROS production and increases the expression of chemokines and interferes with the expression of healing factors by regulating the NF-κB and Wnt pathways.
Effects of polygenic risk score of type 2 diabetes on the hippocampal topological property and episodic memory
Authors: Zhang Yang , Du Xin , Fu Yumeng , Zhao Qiuyue , Wang Zirui , Qin Wen , Zhang Quan
Published: December 2021

Purpose

Type 2 diabetes is associated with a higher risk of dementia. The pathogenesis is complex, partly influenced by genetic factors. The hippocampus is the most vulnerable brain region in individuals with type 2 diabetes. However, whether the genetic risk of type 2 diabetes is associated with the hippocampus and episodic memory remains unclear. This study explored the influence of polygenic risk score (PRS) of type 2 diabetes on the white matter topological properties of the hippocampus among individuals with and without type 2 diabetes and its associations with episodic memory.
Methods

This study included 103 individuals with type 2 diabetes and 114 well-matched individuals without type 2 diabetes. All the participants were genotyped, and a diffusion tensor imaging-based structural network was constructed. PRS was calculated based on a genome-wide association study of type 2 diabetes. The PRS-by-disease interactions on the bilateral hippocampal topological network properties were evaluated by analysis of variance (ANOVA).
Results

There were significant PRS-by-disease interaction effects on the nodal topological properties of the right hippocampus node. In the individuals with type 2 diabetes, the PRS was correlated with the right hippocampal nodal properties, and the nodal properties were correlated with the episodic memory. In addition, the right hippocampal nodal properties mediated the effect of PRS on the episodic memory in individuals with type 2 diabetes.
Conclusion

Our results suggested a gene-brain-cognition biological pathway, which might help understand the neural mechanism of the genetic risk of type 2 diabetes affects episodic memory in type 2 diabetes.
The Effect of Mindfulness Training On Resting-State Networks In Pre-Adolescent Children With Sub-Clinical Anxiety Related Attention Impairments.
Authors: Kennedy Michelle F. , Mohamed Abdalla Z , Schwenn Paul , Beaudequin Denise , Shan Zack , Hermens Daniel F. , Lagopoulos Jim
Published: December 2021

Background: Mindfulness training has been associated with improved attention and affect regulation in preadolescent children with anxiety related attention impairments, however little is known about the underlying neurobiology. This study sought to investigate the impact of mindfulness training on functional connectivity of attention and limbic brain networks in pre-adolescents.Methods: A total of 47 children (aged 9-11 years) participated in a 10-week mindfulness intervention. Anxiety and attention measures and resting-state fMRI were completed at pre- and post-intervention. Sustained attention was measured using the Conners Continuous Performance Test, while the anxiety levels were measured using the Spence Children’s Anxiety Scale. Functional networks were estimated using independent-component analysis, and voxel-based analysis was used to determine the difference between the time-points to identify the effect of the intervention on the functional connectivity. Results: There was a significant decrease in anxiety symptoms and improvement in attention scores following the intervention. From a network perspective, the results showed increased functional connectivity post intervention in the salience and fronto-parietal networks as well as the medial-inferior temporal component of the default mode network. Positive correlations were identified in the fronto-parietal network with Hit Response Time and the Spence Children’s Anxiety Scale total and between the default mode network and Hit Response Time.Conclusions: A 10-week mindfulness intervention in children was associated with a reduction in anxiety related attention impairments, which were underpinned by concomitant changes in functional connectivity.
How Much Is the Lack of Retention Evidence Costing Trial Teams in Ireland and the United Kingdom?
Authors: Murphy Ellen , Shiely Frances , Treweek Shaun
Published: December 2021

BackgroundEvidence to support the use of many retention strategies in clinical trials is lacking. Despite this, trial teams still need to have some form of retention strategy in their trials to try and avoid high attrition rates. This study aimed to estimate how much this lack of retention evidence might be costing trials in Ireland and the United Kingdom.Methods We selected the top ten most routinely used retention strategies by Clinical Trial Units in the United Kingdom and made assumptions as to how each of these strategies was most likely to be conducted and the costs involved. We applied our costing model to a hypothetical trial scenario in both Ireland and the United Kingdom as well as to three published trial protocols. We developed the costing model and calculated the costs in Microsoft Excel.Results Retention strategies can be extremely expensive, some of the costliest interventions included “a timeline of participant visits for sites” (with integrated participant reminders) (€1,418.44 - €108,471.99), “routine site visits by CTU staff” (€777.67 - €14,753.48), and “data collection scheduled with routine care” (€900 - €32,503.25). Others such as “telephone reminders for questionnaire response” (€34.58 - €568.62), “inclusion of pre-paid envelopes” (€93.44 - €942.50), and “targeted recruitment of sites/GPs” (€30 - €1,620) were less costly compared to the other interventions. DiscussionThe resources invested in the use of some retention interventions may outweigh known or imagined benefits on retention. Where benefits are imagined, evaluation should be a priority. Conclusion More evaluation of the effectiveness and cost of trial retention strategies is needed to avoid widespread use of strategies that are both expensive and ineffective.
Microdosimetry Performance of The First Multi-Arrays of 3D-Cylindrical Microdetectors
Authors: Bachiller-Perea Diana , Zhang Mingming , Fleta Celeste , Quirion David , Bassignana Daniela , Gómez Faustino , Guardiola Consuelo
Published: December 2021

Purpose: The present work reports on the microdosimetry measurements performed with the two first multi-arrays of microdosimeters with the highest radiation sensitive surface covered so far. The sensors are based on new silicon-based radiation detectors with a novel 3D cylindrical architecture. Methodology: Each system consists of arrays of independent microdetectors covering 2 mm×2 mm and 0.4 mm×12 cm radiation sensitive areas, the sensor distributions are arranged in layouts of 11×11 microdetectors and 3×3 multi-arrays, respectively. We have performed proton irradiations at several energies to compare the microdosimetry performance of the two systems, which have different spatial resolution and detection surface. The unit-cell of both arrays is a new type of 3D cylindrical diode with a 25 µm diameter and a 20 µm depth that results in a well-defined and isolated radiation sensitive micro-volume etched inside a silicon wafer. Measurements were carried out at the Accélérateur Linéaire et Tandem à Orsay (ALTO) facility by irradiating the two detection systems with monoenergetic proton beams from 6 to 18 MeV at clinical-equivalent fluence rates. Results: The microdosimetry quantities were obtained with a spatial resolution of 200 µm and 600 µm for the 11×11 system and for the 3×3 multi-array system, respectively. Experimental results were compared with Monte Carlo simulations and an overall good agreement was found. Conclusion: We have studied the microdosimetry response under clinical equivalent fluence rate of the first multi-arrays of 3D cylindrical microdetectors covering several centimeters of sensitive area. The good performance of both microdetector arrays demonstrates that this architecture and both configurations can be used clinically as microdosimeters for measuring the lineal energy distributions and, thus, for RBE optimization of hadron therapy treatments. Likewise, the results have shown that the devices can be also employed as a multipurpose device for beam monitoring in particle accelerators.
Effect of Pressure on Alkaline Pretreated Sugar Cane Bagasse for Enhanced Bioethanol Production
Authors: Uddin Azhar , Liaquat Rabia , Abdullah Ali , Khoja Asif Hussain , Muddasar Muhammad , Ullah Sami
Published: December 2021

Global warming has become a major concern as a result of the excessive release of greenhouse gas emissions. An important strategy for achieving carbon neutrality targets is to focus on renewable energy resources. Second generation bioethanol synthesis via sugarcane bagasse (SCB) is another promising approach for the reduction of greenhouse gas emissions. Here in, this study presents the second generation of bioethanol production from sugarcane bagasse with the pretreatment condition adjoined with basic hydrogen peroxide and pressure effect by fermentation using microorganisms Saccharomyces Cerevisiae and Bacillus Subtilis. The results revealed better production through pretreatment at different operational stages through batch fermentation. Different characterization techniques including Scanning Electron Microscopy (SEM), Fourier Transform Infra-Red (FTIR), High Performance Liquid Chromatography (HPLC), and Thermogravimetric Analysis (TGA) results confirmed the better effects of structural changes of hemicellulose, lignin, and cellulose during treatment, weight loss, thermal stability, and higher concentration of the produced bioethanol in the distillate After pretreatment, the conversion of biomass to bioethanol by using Saccharomyces Cerevisiae gives a high production yield (70%), which presents a production of 70g/L from 100g of SCB at the end of 72 h and a yield of bioethanol (0.7g/g) of SCB confirmed through gas chromatography/mass spectrometry qualitative analysis (GC/MS). The pretreatment conditions of alkaline hydrogen peroxide (H2O2) were optimized to the values 3h, 50°C, 60 psi, pH 8.6, and 150 rpm. This study sheds light on the effects of pretreatment conditions for bioethanol production from sugarcane bagasse.
Quantum Surprises from the Watson-Crick and Hoogsteen G·C Nucleobase Pairs: A Comprehensive QM/QTAIM Investigation
Authors: Brovarets Ol’ha O. , Muradova Alona , Hovorun Dmytro M.
Published: December 2021

In this study at the MP2/6-311++G(d,p)//B3LYP/6-311++G(d,p) level of theory in the isolated state it was revealed 14 novel physico-chemical mechanisms of the tautomerization of the G·C nucleotide base pairs in the Watson-Crick G·C(WC) / G*·C*(WC), reverse Watson-Crick G*·C*(rWC) / G·C*O2(rWC), Hoogsteen G*t·C*(H) / G*N7·C(H) or reverse Hoogsteen G*t·C*(rH) / G*tN7·C(rH) configurations into the wobble (wWC, wH) and reverse wobble (rwWC, rwН) base pairs: 1. G·C(WC)↔G·C*(rwWC), 2./3. G*·C*(WC)↔G·C*(rwWC)/G*N2·C*(rwWC), 4. G*·C*(rWC)↔G*·C(wWC), 5. G·C*O2(rWC)↔G·C*(wWC); 6./7./8./9. G*t·C*(H)↔G*t·C(rwН)/G*t·C*O2(wH)/G*t·C*O2(rwН)/G*tN7·C*(rwН)↔G*t·C*O2(rwН), 10. G*N7·C(H)↔G*t·C(wH) amino, 11./12. G*t·C*(rH)↔G*N7·C*(wН)/G*t·C(wН), 13. G*tN7·C(rH)↔G*tN7·C*(wН)↔G*t·C(wН) and 14. G*N7·C*(rwH)↔G*N7·C*(rwH) perp↔G-·C+(wH)↔G*t·C(rwН) reaction pathways. It was established that the presence in the base pair of the two anti-parallel neighboring H-bonds is a necessary and sufficient condition for the implementation of such transformations, since it enables intermolecular proton transfer between the bases inside the base pair. It was found out that these tautomeric transitions are controlled by the TSs with quasi-orthogonal structure, which are tight G+·C-/G-·C+ ion pairs, joined by at least two parallel intermolecular H-bonds, connected on a common negatively charged endocyclic N-/C- atoms – proton acceptor. All reaction pathways have been reliably confirmed. These transitions are accompanied by the changing of the mutual cis-orientation of the N9H and N1H glycosidic bonds of the bases on the trans-orientation and vice versa. These data complement the reported earlier mechanisms of the tautomerisations of the classical A·T and G·C DNA base pairs. Experimental verification of the novel G·C nucleobase pairs is looking as an attractive task for the future research.
Analysis of the Influence of Lifestyle on Liver Dysfunction Based on NAHANES Database
Authors: XU Yan , LIU Rong
Published: December 2021

ObjectiveTo find out the influence of various factors in life style on liver dysfunction, put forward reasonable suggestions on prevention of liver dysfunction.MethodsDatasets from 2017 to March 2020 NHANES (National Health and Nutrition Examination Surveys) required for the analysis were downloaded from the NHANES web site and R 4.1.1. Software was used for data analysis. Survey Design Logisitic regression was used to analyze the influence of various factors on liver dysfunction and screen the risk factors.Resultshypertension, depression, and sedentary activity are risk factors for liver dysfunction, while reducing salt in diet and vigorous recreational activities were protective factors for liver dysfunction. The inflection points of blood pressure, BMI and sedentary activity were 98.33mmHg, 30.6kg/m2, 420min, respectively.ConclusionsBlood pressure, BMI, mood and sedentary behavior are risk factors for liver dysfunction. We suggest that keeping MAP level at 70-98.33mmHg, controlling BMI < 30kg/m2, maintaining a positive attitude, and sedentary time less than 420 min per day are more conducive to reducing the risk of liver dysfunction.
A SINS/DVL/USBL Integrated Navigation and Positioning IoT System with Multiple Sources Fusion and Federated Kalman Filter
Authors: Luo Qinghua , Yan Xiaozhen , Wang Chenxu , Shao Yang , Zhou Zhiquan , Li Jianfeng , Hu Cong , Wang Chuntao , Ding Jinfeng
Published: December 2021

The navigation and positioning subsystem offers important position information for an autonomous underwater vehicle (AUV) system. It plays a crucial role during the underwater exploration and operations of AUV. Many scholars research underwater navigation and positioning. And many promising methods and systems were presented. However, as the diversity of ocean environment, the random drift of the gyroscope, error accumulation, the diversity of tasks, and other negative factors, the navigation and positioning result is uncertain and incredible. The accuracy, stability and robustness are not guaranteed, which can not meet the increasing application requirement. Therefore, we put forward a SINS/DVL/USBL integrated navigation and positioning IoT system with multiple resource fusion and a federated Kalman filter. In this method, we first present an improved SINS/DVL combined subsystem with filtering gain compensation strategy. The accuracy and stability of the navigation and position system can be enhanced. Secondly, We proposed a USBL positioning subsystem with the Kalman filtering acoustic signals to improve USBL positioning performance. Lastly, we present a federated Kalman Filter to fuse the positioning information from the SINS/DVL combined positioning subsystem and the USBL positioning subsystem. Through the above three methods, we can improve the positioning accuracy and robustness. Comprehensive simulation results indicated the feasibility and effectiveness of the proposed SINS/DVL/USBL integrated navigation and positioning system.
Profiling Gut Microbiota and Bile Acid Metabolism in Critically ill Children
Authors: Kean Iain Robert Louis , Wagner Joseph , Wijeyesekera Anisha , De Goffau Marcus , Thurston Sarah , Clark John A. , White Deborah K. , Ridout Jenna , Agrawal Shruti , Kayani Riaz , O’Donnell Roddy , Ramnarayan Padmanabhan , Peters Mark J. , Klein Nigel , Holmes Elaine , Parkhill Julian , Baker Stephen , Pathan Nazima
Published: December 2021

Broad-spectrum antimicrobial use during the treatment of critical illness influences gastrointestinal fermentation endpoints, host immune response and metabolic activity including the conversion of primary to secondary bile acids. We previously observed reduced fermentation capacity in the faecal microbiota of critically ill children upon hospital admission. Here, we further explore the timecourse of the relationship between the microbiome and bile acid profile in faecal samples collected from critically ill children. The microbiome was assayed by sequencing of the 16s rRNA gene, and faecal water bile acids were measured by liquid chromatography mass spectrometry. In comparison to admission faecal samples, members of the Lachnospiraceae recovered during the late-acute phase (days 8-10) of hospitalisation. Patients with infections had a lower proportion of Lachnospiraceae in their gut microbiota than controls and patients with primary admitting diagnoses. Keystone species linked to ecological recovery were observed to decline with the length of PICU admission. These species were further suppressed in patients with systemic infection, respiratory failure, and undergoing surgery. Bile acid composition recovers quickly after intervention for critical illness which may be aided by the compositional shift in Lachnospiraceae. Our findings suggest gut microbiota recovery can be readily assessed via measurement of faecal bile acids.