bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of Methodological Approaches for Examination of Particularly Dangerous Infectious Diseases Agents by Means of Atomic Power Microscopy
Authors: Utkin D. V. , Kuznetsov O. S. , Erokhin P. S. , Spitsyn A. N. , Volokh O. A. , Osina N. A.
Published: December 2018

The atomic power microscopy (APM) is used to study the cell surface structure of particularly dangerous infectious diseases agents and to carry out the morphometric analysis. APM shows similar results with scanning electron microscopy. However, its application makes it possible to avoid time-consuming and labour-intensive procedures of samples preparing for testing by fixation, dehydration and sputtering of conducting layer. Methodological approach has been elaborated for preparing and analysis of samples of agents of particularly dangerous infectious diseases by means of APM. This approach includes a selection of optimal substrate, mode of disinfection and scanning of samples.
Development of DNA-Biochip for Identification of Influenza A Virus Subtypes
Authors: Shikov A. N. , Sergeeva E. I. , Demina O. K. , Ternovoy V. A. , Ryabinin V. V. , Kostina E. V. , Malkova E. M. , Sinyakov A. N. , Agaphonov A. P.
Published: December 2018

Developed was the DNA-biochip to identify subtypes of influenza A virus, pathogenic for humans. Microchip was capable of detecting H1, H3, H5-subtypes of hemagglutinin (including H1-subtype of pandemic A/H1N1(2009) influenza virus ) and neuraminidase subtypes N1,N2 of influenza virus. This microchip was successfully tested on the strains of A/H5N1 highly pathogenic avian influenza virus, A/H1N1(2009) pandemic influenza virus, A/H1N1 and A/H3N2 seasonal influenza viruses.
Complications of Sanitary-Epidemiological Situation and Risk of Emergency Situations Appearance in the Sphere of Sanitary and Epidemiological Well-Being in the Natural and Anthropogenic Catastrophes
Authors: Karnaukhov I. G. , Starshinov V. A. , Toporkov V. P. , Toporkov A. V. , Korotkov V. B.
Published: December 2018

Presented is the survey of emergency in the sphere of sanitary and epidemiological well-being of the population as per reported cases and data of the monitoring which has been carried out in 2010. Natural and anthropogenic disasters where such emergency is appeared or can appear are considered too. Natural catastrophes - earthquakes, volcanic explosions, overflow water, tsunami, dry spell and anthropogenic catastrophes - humanitarian, technogenic catastrophes, bioterroristic acts, accidents, related to operation with pathogen biological agents are examined in this review as the conditions preceding the emergency. Nosological entities for the infection diseases, which are most common in natural and anthropogenic catastrophes as well as conditions and factors predisposing to their activation, are identified. Shown is the data of adverse epidemiological situation caused by natural and anthropogenic catastrophes and the examples of use of the biological agents for terroristic aims.
Verification of Mathematical Models of Plague
Authors: Lopatin A. A. , Kuklev E. V. , Safronov V. A. , Razdorsky A. S. , Samoilova L. V. , Toporkov V. P.
Published: December 2018

Mathematic modeling and prognostication of infectious diseases epidemic process is a promising trend of epidemiologic investigations. The complex of mathematic models (SEIRF type) of plague epidemic process was developed for this purpose by the Russian Research Anti-Plague Institute “Microbe” and laboratory of epidemiologic cybernetics of N.F.Gamaleya Institute for Epidemiology and Microbiology. The data on the plague outbreak in 1945 in the rural settlement Avan’ (Aral region of Kzyl-Orda district of Kazakh SSR) were used to test working efficiency of this complex. The data analysis permitted to obtain the starting and boundary conditions for epidemic process modeling. In the process of modeling the mathematical models of epidemic process of plague with various ways of infection transmission for each epidemic focus in regard with historical data were used. The data were processed by the analytical platform Deductor 5.1. Identified was strong positive correlation between estimated and historical data – r = +0,71. The results received testify that mathematic models are effective and give high degree of confidence. They can be used to receive quantitative characteristics of prognosis for plague epidemic process development with different transmission routes considering that anti-epidemic measures have been taken.
Validation of Technological Process of Production of Liquid Brucellosis Diagnosticum for Agglutination Reaction, Suspension for Diagnostic Purposes
Authors: Kogotkova O. I. , Zuenko A. A. , Zharnikova I. V. , Gridina T. M. , Novakov P. B. , Lyapustina L. V. , Lyamkin G. I. , Vilinskaya S. V. , Kulichenko A. N.
Published: December 2018

Presented are the results of validation of technological process of production of brucellosis diagnosticum
Epizootiological and Epidemiological Situation in Tularemia Natural Foci in the Territory of the Central Federal District of the Russian Federation from 1992 to 2011
Authors: Popov V. P. , Orlov D. S. , Bezsmertny V. E.
Published: December 2018

Outlined is a brief characteristics on epizootic and epidemic activity of tularemia natural foci in the territory of the Central Federal district of the Russian Federation over a period of 1992–2012. Implementation of GIS-technologies has made it possible to design retrospective map of the sites of the district population infectioning in 2005, and to distinguish the regions that are of a great epidemic significance.
Analysis of Genetic Determination of
Vibrio cholerae Tweenase Activity
Authors: Mazrukho A. B. , Monakhova E. V. , Duvanova O. V. , Mishan’Kin B. N. , Kruglikov V. D.
Published: December 2018

Studied are Vibrio cholerae of different serogroups on the presence of cef (CHO cell elongating factor) gene and activity against tweens and tributyrin using HDS-agar, prepared on the basis of bakery yeast pancreatic digest. Determined is the fact that all cef-positive strains hydrolyze tweens 20, 40, 60, 80, 85 and all but toxigenic V.cholerae O139, hydrolyze tributyrin. In contrast, non-toxigenic cef-negative strains of O139 serogroup, are active only against the latter. Apparently, the tweenase activity of Vibrio cholerae is provided, partly, by Cef, and the ability to hydrolyze tributyrin is the result of combined activity of Cef and other ferments. Shown is the efficiency of HDS-agar application to determine these characteristics.
Two Contact Problems for Annular Rigid Stamp on an Elastic Half Space
Authors: Bosakov S. V.
Published: December 2018

The paper presents solutions of two contact problems for the annular plate die on an elastic half-space under the action of axisymmetrically applied force and moment. Such problems usually arise in the calculation of rigid foundations with the sole of the annular shape in chimneys, cooling towers, water towers and other high-rise buildings on the wind load and the load from its own weight. Both problems are formulated in the form of triple integral equations, which are reduced to one integral equation by the method of substitution. In the case of the axisymmetric problem, the kernel of the integral equation depends on the product of three Bessel functions. Using the formula to represent two Bessel functions in the form of a double row on the works of hypergeometric functions Bessel function, the problem reduces to a functional equation that connects the movement of the stamp with the unknown coefficients of the distribution of contact stresses. The resulting functional equation is reduced to an infinite system of linear algebraic equations, which is solved by truncation. Under the action of a moment on the annular plate  die, the distribution of contact stresses is searched as a series by the products of the Legendre attached functions with a weight corresponding to the features in the contact stresses at the die edges. Using the spectral G. Ya. Popov ratio for the ring plate, the problem is again reduced to an infinite system of linear algebraic equations, which is also solved by the truncation method. Two examples of calculations for an annular plate die on an elastic half-space on the action of axisymmetrically applied force and moment are given. A comparison of the results of calculations on the proposed approach with the results for the round stamp and for the annular  stamp with the solutions of other authors is made.
Hydraulic Calculation of Copper Pipelines for Heating and Internal Gas Supply Systems
Authors: Rutkowski M. A. , Shybeka A. S. , Halynia K. I.
Published: December 2018

Copper pipelines are widely used while designing modern heating systems, water and gas supply of residential buildings and these pipelines have a number of advantages in comparison with steel and plastic pipelines. The main reason for limited use of copper pipes is their cost which is practically twice as much as cost of steel pipes, and four times higher than the coat of polypropylene pipes. Modern design standards in Belarus do not contain specific requirements for calculation of copper pipelines. Therefore hydraulic calculation of heating systems has been considered and analyzed on the basis of norms of Russian Federation. The drawback of all theoretical calculations presented in Russian standards is their abstraction from general laws of fluid and gas mechanics. For this reason theoretical foundations of hydraulic calculations for all pipelines have been considered and expressions have been given for determination of pressure loss due to friction and in local loss. Main flow regimes have been considered on the basis of I. Nikuradze’s experiments in order to determine linear losses and dependences for determination of friction coefficient in laminar, transient and turbulent modes have been presented in the paper. Values of local loss coefficient for main pipeline elements are given for calculation of pressure loss and the paper also shows determination of value z with due account of equipment capacity. An analysis of nomograms presented in Russian norms and researches has been carried out; shortcomings have been revealed and the ways directed on their perfection have been found. Nomograms for calculation of specific pressure losses in heating systems and internal gas supply have been constructed on the basis of natural gas and propane with due account of dependences presented in the paper. Nomograms for finding equivalent length of a single local loss have been constructed for calculation of internal gas supply systems.
HPV-FASTER: Combined strategies of HPV vaccination and HPV screening towards a one visit for cervical cancer preventive campaigns
Authors: Bosch F Xavier , Robles Claudia
Published: December 2018

[Not available]
Test
Authors: Test Test
Published: December 2018

This is a test paper.
Redistribution of the Corneal Epithelium After Overnight Wear of Orthokeratology Contact Lenses for Myopia Reduction
Authors: Zhang Ju , Li Jing , Li Xiaofeng , Li Fengjie , Wang Ting
Published: December 2018

Background: To investigate changes in the corneal epithelium topographic map in juvenile myopia after overnight wear of orthokeratology lenses (OK).
Methods: A total of 53 juveniles (53 right eyes) successfully wore OK and were reviewed in a timely manner from January 2016 to July 2017. Epithelial and corneal data were obtained by optical coherence tomography (OCT). Changes in uncorrected visual acuity, diopter, corneal refractive power, and epithelial and corneal thickness were analyzed before and after 1 day, 1 week, and 1 mo of overnight wear of OK.
Results: The corneal epithelium was reshaped after 1 day of OK; the myopia degree was reduced, and uncorrected visual acuity reached 0 logMAR at 1 week. The central (2-mm) average epithelial thickness was 52.04±2.35 μm, 49.25±2.67 μm, 45.91±2.80 μm, and 47.53±3.44 μm before and after 1 day, 1 week, and 1 mo of OK, respectively (t=10.122, 10.782, and 10.673, respectively, P≤0.001). The central epithelium appeared to have a thinning trend, which was obvious at 1 week, when the average thinning of 6.13±1.67 μm accounted for approximately 11.78%±3.21% of the total epithelium thickness. The epithelial thickness of the reverse curve zone was 51.83±2.49 μm, 57.62±3.01 μm, 59.43±3.19 μm, and 60.22±2.75 μm before and after 1 day, 1 week, and 1 mo of OK, respectively, showing a significant increase over time (t=5.325, 6.177, and 6.312, respectively, P≤0.001).
Conclusion: In the early stage of OK, the corneal epithelium was redistributed very quickly. The central epithelium became thin, while the epithelium of the reverse curve zone correspondingly thickened and achieved a plateau at 1 mo. Epithelial redistribution guaranteed uncorrected visual acuity after removal of OK.
Recurrent post-partum rhombencephalitis associated with anti-centromere antibody: A case report
Authors: Jin Andy , Mamelona Jean , Harper Byrne , Marrero Alier
Published: December 2018

Background
Rhombencephalitis (RE) is a serious condition of the brain with multiple etiologies. We report a unique case of recurrent, postpartum RE that is associated with positive anti-centromere antibody (ACA). A discussion of the case, current literature on autoimmune RE and related autoantibodies are reviewed.
Case Presentation
A healthy 33-year-old Caucasian patient (gravida 2, para 2) had two episodes of progressive focal neurological deficits during postpartum periods. Signs and symptoms included right-sided dysmetria, adiadochokinesia, weakness, ataxia, and photophobia. MRI revealed rhombencephalitis involving the mesencephalon. Extensive and comprehensive investigations using blood and cerebrospinal fluid (CSF) were consistently positive only for ACA. The first episode was successfully treated with empiric antimicrobial agents and steroid. Given the negative infectious work up with the prior episode and the nearly identical clinical presentations, the second episode was treated with corticosteroid only. This led to complete resolution of her symptoms and reversal of the brain magnetic resonance imaging (MRI) lesions.
Conclusion
To the authors’ knowledge, this is the first report of a primary autoimmune RE during postpartum period that is associated with ACA. Immunologic causes should be considered early with any encephalitis. Given the risk of recurrence, relapse, and neurologic deterioration, regular monitoring is recommended, especially for female patients of child-bearing age. Consistent with the current literature on autoimmune RE, steroid seems to be an effective treatment for ACA-associated RE.
Keywords: Autoimmune disease, rhombencephalitis, anti-centromere antibody, connective tissue disease, case report
One-year Versus Five-year Hospital Readmission after Ischemic Stroke and TIA
Authors: Bjerkreim Anna Therese , Naess Halvor , Khanevski Andrej Netland , Thomassen Lars , Waje-Andreassen Ulrike , Logallo Nicola
Published: December 2018

Background: The burden of readmission after stroke is substantial, but little knowledge exists on factors associated with long-term readmission after stroke. In a cohort composing patients with ischemic stroke and transient ischemic attack (TIA), we examined and compared factors associated with readmission within 1 year and first readmission during year 2-5.
Methods: Patients with ischemic stroke or TIA who were discharged alive between July 2007 and October 2012, were followed for five years by review of medical charts. Timing and cause of the first unplanned readmission were registered. Cox regression was used to identify independent risk factors for readmission within 1 year and first readmission during year 2-5 after discharge.
Results: The cohort included 1453 patients, of whom 568 (39.1%) were readmitted within 1 year. Of the 830 patients that were alive and without readmission 1 year after discharge, 439 (52.9%) were readmitted within 5 years. Patients readmitted within 1 year were older, had more severe strokes, poorer functional outcome, and a higher occurrence of complications during index admission than patients readmitted during years 2-5. Cardiovascular comorbidity did not differ between the two groups of readmitted patients. Higher age, poorer functional outcome, coronary artery disease and hypertension were independently associated with first readmission within both 1 year and during year 2-5. Peripheral artery disease was independently associated with readmission within 1 year, and atrial fibrillation was associated with first readmission during year 2-5.
Conclusions: More than half of all patients who survived the first year after stroke without any readmissions were readmitted within 5 years. Patients readmitted within 1 year and between years 2-5 shared many risk factors for readmission, but they differed in age, functional outcome and occurrence of complications during the index admission.
Molecular and Morpho‐Agronomic Characterization among Introduced Bristle Oat Collection Using ISSR Fingerprinting
Authors: Ghariani Salma , Amari Marwen , Charfeddine Amina , Chakroun Mohamed , Trifi‐Farah Neila
Published: December 2018

Core Ideas

The genetic diversity of Avena strigosa was examined in order to clarify its phylogenic relationships and analyze the domestication of these well‐adapted ecotypes in the Tunisian environment.

The analysis of molecular variance revealed that the 91% of the variation was within groups of accessions according to their geographic origins and only 9% appeared among groups.

Three analyses revealed a scattered distribution, independent of the geographic origin.

Knowledge about the genetic variation of bristle oat (Avena strigosa Schreb.) cultivars is helpful for the development of conservation strategies to molecularly assist selection to improve the introduced germplasm in the Tunisian environment. The present study examines the genetic diversity among 22 accessions of A. strigosa and clarifies its phylogenic relationships to analyze the domestication of these well‐adapted ecotypes. Twenty‐two accessions of diploid A. strigosa sampled from 22 various geographical locations were analyzed with 20 universal inter‐simple sequence repeat (ISSR) primers. Twelve morpho‐agronomic traits were scored and all data were compiled to establish the repartition of the total ISSR variation and to analyze the genetic structure of the accessions under study. Out of 275 bands, 252 were recorded as polymorphic. The analysis of molecular variance revealed that 91% of the variation was within groups of accessions according to their geographic origins. In addition, three analyses (Bayesian, principal component analysis, and unweighted pair group method with arithmetic mean) revealed a confused distribution, independent of the geographic origin. Therefore, it may be concluded that the bristle oat accessions are interrelated in spite of their agronomic divergence. Avena strigosa may be a potential forage crop that is suited to marginal Tunisian environments.
Effect of Biochar Application on Hydraulic Properties of Sandy Soil under Dry and Wet Conditions
Authors: Chen Chong , Wang Ruiqi , Shang Jianying , Liu Kesi , Irshad Muhammad Kashif , Hu Kelin , Arthur Emmanuel
Published: December 2018

Core Ideas

High doses of biochar increased water retention of sandy soil in both dry and wet conditions.

Larger sized biochar decreased water retention at field capacity (FC).

High‐pyrolysis biochar could increase water retention at FC and in dry conditions.

We investigated the impact of biochar application on the evaporation rate of sandy soil.

Knowledge of soil hydraulic properties under dry (below permanent wilting point) and wet (from saturation to permanent wilting point) conditions is helpful for evaluating soil physical quality and modeling the movement of the substances (water and nutrients) in biochar‐amended soils. To investigate the effect of biochar application on hydraulic properties of sandy soil under dry and wet conditions, water retention in wet conditions and soil drying curves from wet to dry conditions were measured under different application rates (1, 3, and 5%, w/w), particle sizes (<0.25, 0.25–0.5, 0.5–1, and 1–2 mm), and pyrolysis temperatures (300, 450, and 600°C) of wheat (Triticum aestivum L.) straw‐derived biochar. Results showed that when higher rate biochar (3 and 5%) was applied into the sandy soil, water retention became higher under dry and wet conditions. Biochar application with a larger particle size (0.5–1 and 1–2 mm) increased water retention under saturation and dry conditions but decreased water retention at field capacity. Sandy soil amended with biochar at the higher pyrolysis temperatures (450 and 600°C) had higher water retention under field capacity or dry conditions. Increasing biochar application rate, particle size, and pyrolysis temperature decreased the evaporation rate of sandy soil under dry conditions. Our findings suggested that hydraulic properties of the sand–biochar mixture were mainly determined by biochar properties under dry conditions and were highly related to the interpores between particles under wet condition.
Effect of Soil Texture on Estimates of Soil‐Column Carbon Dioxide Flux Comparing Chamber and Gradient Methods
Authors: Yang Xiaoli , Fan Jun , Jones Scott B.
Published: December 2018

Core Ideas

Carbon dioxide efflux from soil of different textures was compared.

The dominant mechanism of CO2 efflux changed from coarse to fine soil.

The gradient method should be used very carefully in coarse soils.

A key component of the global C balance includes soil CO2 emissions. Both chamber‐ and gradient‐based methods have been effectively used for determination of soil CO2 efflux. The gradient‐based method, however, is installed belowground and requires assessment of the soil gas diffusion coefficient, which varies with soil properties such as soil texture. We hypothesized that the gradient and chamber methods would yield similar results when soil texture was appropriately accounted for. Our objective was to evaluate soil gas efflux by applying both methods in three different soil textures (loam, silty loam, and silty clay loam) packed in laboratory soil columns. Water was incrementally applied to soil surfaces while volumetric soil water contents (VWC) and CO2 concentrations were continually monitored at target locations in parallel with surface chamber measurements. The Soil Air Phase Individual Resistances (SAPHIR) model was used to determine the relative gas diffusion coefficients (DP/D0). The gradient‐based CO2 effluxes were compared with estimates derived from the CO2–efflux chamber. Soil CO2 effluxes estimated using the gradient method approximated those using the chamber for all three soil textures. The particle shape factor (p) in the SAPHIR model varied with silt content of the three soils. The SAPHIR model's water blockage factor (w) also varied with soil texture to a lesser extent. Under the repacked soil condition, these results confirm that the gradient method can provide consistent CO2 measurements in comparison with chamber‐based results from soils of varied texture when the diffusion coefficient takes into account the effects of soil texture. The gradient method should be used cautiously in coarse‐textured soils.
Organotypic Brain Slices of ADULT Transgenic Mice: A Tool to Study Alzheimer’s Disease
Authors: Humpel Christian
Published: December 2018

Transgenic mice have been extensively used to study the Alzheimer pathology. In order to reduce, refine and replace (3Rs) the number of animals, ex vivo cultures are used and optimized. Organotypic brain slices are the most potent ex vivo slice culture models, keeping the 3-dimensional structure of the brain and being closest to the in vivo situation. Organotypic brain slice cultures have been used for many decades but were mainly prepared from postnatal (day 8-10) old rats or mice. More recent work (including our lab) now aims to culture organotypic brain slices from adult mice including transgenic mice. Especially in Alzheimer´s disease research, brain slices from adult transgenic mice will be useful to study beta-amyloid plaques, tau pathology and glial activation. This review will summarize the studies using organotypic brain slice cultures from adult mice to mimic Alzheimer's disease and will highlight advantages and also pitfalls using this technique.
Latest Evidence on the Role of Multiparametric Magnetic Resonance Imaging in Active Surveillance for Insignificant Prostate Cancer: A Systematic Review
Authors: Cantiello Francesco , Manno Stefano , Russo Giorgio I. , Cimino Sebastiano , Privitera Salvatore , Morgia Giuseppe , Cicione Antonio , Damiano Rocco
Published: December 2018

Objective:

Multiparametric Magnetic Resonance Imaging (mpMRI) has become a very useful tool in
the management of PCa. Particularly, there is a great interest in using mpMRI for men on Active Surveillance
(AS) for low risk PCa. The aim of this systematic review was to critically review the latest literature concerning
the role of mpMRI in this clinical setting, underlying current strengths and weakness.

Evidence Acquisition:

A comprehensive literature research for English-language original and review articles
was carried out using the National Center for Biotechnology Information PubMed database with the aim to
identify studies pertaining to mpMRI for AS in low risk PCa patients. The following search terms were used:
active surveillance, prostate cancer and multiparametric magnetic resonance imaging.

Evidence Synthesis:

Data from 28 recent original studies and reviews were reviewed. We only considered studies
on the use of mpMRI in selecting AS patients and during AS follow-up, in order to solve two important
questions: -Can mpMRI have a role in improving the detection of clinically significant disease, better selecting
AS patients? -Can mpMRI identify the progression of disease and, consequently, be used during AS follow-up?

Conclusions:

mpMRI is useful to better select the ideal candidates to AS and to monitor them during follow-up.
However, despite many advantages, there are yet important limitations to detect all clinically significant PCa
and to better define mpMRI-radiological progression during AS. Further larger prospective studies are needed to
definitively solve these important problems.
Synthesis, Cytotoxicity and Antioxidant Activity of New Analogs of RC-121 Synthetic Derivatives of Somatostatin
Authors: Naydenova Emilia , Wesselinova Diana , Staykova Svetlana , Goshev Ivan , Vezenkov Ljubomir
Published: December 2018

Background:

Based on the structure of RC-121 (D-Phe-c (Cys-Tyr-D-Trp-Lys-Val-Cys)-Thr-NH2, -
synthetic derivatives of somatostatin), some analogs were synthesized and tested for in vitro cytotoxic and antioxidant
activity.

Objectives:

The new analogs were modifyed at position 5 with Dap (diaminopropanoic acid), Dab (diaminobutanoic
acid) and Orn and at position 6 with the unnatural amino acids Tle (t-leucine).

Methods:

The in vitro cytotoxic effects of the substances were investigated against a panel of human tumor cell
lines HT-29 (Human Colorectal Cancer Cell Line), MDA-MB-23 (Human Breast Cancer Cell Line), Hep G-2
(Human Hepatocellular Carcinoma Cell Line) and HeLa (cervical cancer cell line). The antioxidant capacities
were tested by ORAC (Oxygen Radical Antioxidant Capacity) and HORAC (Hydroxyl Radical Averting Capacity)
methods.

Results:

All substances expressed significantly higher antioxidant capacity by comparison with galic acid and
Trolox. All substances showed considerable antioxidant capacity as well. Compound 2T (D-Phe-c(Cys-Tyr-DTrp-
Dap-Tle-Cys)-Thr-NH2)had the highest antioxidant effect. The compound 4T (D-Phe-c(Cys-Tyr-D-Trp-
Orn-Tle-Cys)-Thr-NH2) displayed antiproliferative effect on HeLa cells with IC50 30 µM. The peptide analog 3T
(D-Phe-c(Cys-Tyr-D-Trp-Lys-Tle-Cys)-Thr-NH2) exerted the most pronounced inhibition on the cell vitality up
to 53%, 56% and 65% resp. against MDA-MB-23, Hep G-2, HeLa in the higher tested concentration.

Conclusion:

The somatostatin analogs showed moderate influence on the vitality of different tumor cells and
could be used in changing their pathology.
Characterization of Cells Interactions with Patterned Azopolymer-Based Materials using SEM, AFM and Video Microscopy
Authors: Barillé Régis , Codron Philippe , Mabilleau Guillaume , Manero Florence , Mallet Romain , Zielinska Sonia , Ortyl Ewelina , Eyer Joël , Letournel Franck
Published: December 2018

Introduction:

Artificial Extracellular Matrices (ECMs) are promising tools for the study of cell behaviors.

Methods:

Here, we report a protocol for the use of a reconfigurable biocompatible azopolymer thin film through a photoinduced reconfigurable structuration of its surface to study nerve growth, differentiation and cell guidance.

Results & Discussion:

We show that this protocol combined with a spontaneous self-photoinduced polymer is suitable for time-lapse fluorescence video microscopy and can be easily adapted to electron microscopy techniques (SEM) and near-field imaging techniques (AFM).
Molecular Study of Enterotoxins Genes of Staphylococcus aureus Isolated from Patients in Intensive Care Units
Authors: Zaki Maysaa El Sayed , Rizk Mohamed A. , Bakr Asmaa O.
Published: December 2018

Introduction:

Staphylococcus aureusis responsible for healthcare-associated sepsis. Various virulence genes may be responsible for the severity of the outcome of this infection.

Aims & objectives:

Therefore, the aim of the present study was to investigate the prevalence of enterotoxin genes inS .aureusassociated with HCA sepsis affecting adult's patients in Intensive Care Units (ICU).

Materials & Methods:

The study is a cross-sectional observational study included 432 patients admitted to intensive care units with Healthcare-Associated Infections (HCAIs). The patients were subjected to blood culture and identification of the isolated bacteria according to the standard microbiological method. Isolates identified asS. aureuswere subjected to molecular studies for the detection of meca gene and enterotoxins genes by multiplex Polymerase Chain Reaction (PCR).

Results & Discussion:

Blood culture was positive in 170 of the patients (39.3%). 47 of the blood culture yieldS. aureu.Regarding studies of virulence genes in isolatedS. aureus, the most frequent genes were seg (61.7%), mecA (46.8%) and sea (42.6%). The less frequent genes were tst and eta 6.4% for each. Analysis of demographic, clinical and virulence genes of isolatedS. aureusto HAIs outcome reveals that the virulence genes namely mec, sei and tst ofS. aureuswere the only significant factors associated with death The distribution of enterotoxigenic genes among MRSA and MSSA shows a significant association between seg and sei genes in MRSA isolates.

Conclusion:

The present study highlights the prevalence of enterotoxin genes in isolated S.aureus from patients with hospital-acquired infections in intensive care units in a tertiary hospital. There was high prevalence as about two-thirds of the isolates harbor seg and sea genes in addition to methicillin resistance gene mecA. The fatal outcome of sepsis in those patients was associated with the presence of enterotoxin genes sei and tst. There is a need for screening patients admitted to intensive care units forS. aureusharboring these genes for proper management and application of isolation and contact guidelines of infection control
Myeloid-derived Suppressor Cells in Cancer: A Review on the Pathogenesis and Therapeutic Potentials
Authors: Richard Seidu A.
Published: December 2018

Myeloid-Derived Suppressor Cells (MDSCs) are multifarious group of immature cells that arise from the myeloid and amass in individuals with cancer, sepsis, burns, or chronic inflammation. It has been evidenced that these group of cells are efficient in modifying adaptive and innate immune responses, coherent with their assumed key biological roles. It is evidenced that MDSCs inter-communicate with Tumor-Associated Macrophages (TAM), Tumor-Associated Neutrophils (TAN), Dendritic Cells (DCs), Receptor for Advanced Glycation End-products (RAGE), Toll-Like Receptors (TLRs), Matrix Metalloproteinase (MMPs) as well as High Mobility Group Box 1 (HMGB1) during carcinogenesis. This interaction although elaborated in various studies and reviews still does not explain in details as to how their interplay results in cancer pathogenesis. We noted that MDSC contributed to cancer immune suppressionviaTLR-4 receptor and lipopolysaccharideas (LPS). Furthermore, MDSC contributed to cancer developmentviaMMPs (MMP-9 and MMP1-12) as well as RAGE. In the cancer microenvironment, HMGB1-driven MDSC amassment expedites cancer development and metastasisviaPMN-MDSCs, macrophages, DCs and Immature Myeloid Cells (IMC). Also, HMGB1 intermediation with MDSCsviaRAGE and/or TLR-4 leading to cancer development. Nevertheless, MDSCs have already proven potent in some cancers and are currently been used as treatment options although further studies are needed in some other cancers. Our review, therefore, explores the pivotal pathogenic and therapeutic roles of MDSCs in cancer.
Apresentação
Authors: Aldé Alessandra , Cervi Emerson Urizzi , Chagas Viktor
Published: December 2018

Apresentação
Identifying Sleep-Deprived Authors of Tweets: Prospective Study (Preprint)
Authors: Melvin Sara , Jamal Amanda , Hill Kaitlyn , Wang Wei , Young Sean D
Published: December 2018

BACKGROUND

Social media data can be explored as a tool to detect sleep deprivation. First-year undergraduate students in their first quarter were invited to wear sleep-tracking devices (Basis; Intel), allow us to follow them on Twitter, and complete weekly surveys regarding their sleep.

OBJECTIVE

This study aimed to determine whether social media data can be used to monitor sleep deprivation.

METHODS

The sleep data obtained from the device were utilized to create a tiredness model that aided in labeling the tweets as sleep deprived or not at the time of posting. Labeled data were used to train and test a gated recurrent unit (GRU) neural network as to whether or not study participants were sleep deprived at the time of posting.

RESULTS

Results from the GRU neural network suggest that it is possible to classify the sleep-deprivation status of a tweet’s author with an average area under the curve of 0.68.

CONCLUSIONS

It is feasible to use social media to identify students’ sleep deprivation. The results add to the body of research suggesting that social media data should be further explored as a potential source for monitoring health.
PELATIHAN KELOMPOK PEDULI HIPERTENSI SEBAGAI UPAYA PENINGKATAN KUALITAS HIDUP PASIEN HIPERTENSI DI RAJAMANDALA KULON BANDUNG BARAT
Authors: Sari Citra Windani Mambang
Published: December 2018

Permasalahan kesehatan di Kabupaten Bandung Barat sangat kompleks. Salah satunya adalah Hipertensi merupakan salah satu penyakit kronis yang angka kejadian dan komplikasi yang diakibatkannya terus meningkat dari waktu ke waktu. Kondisi tenaga kesehatan yang terbatas membutuhkan keterlibatan kader kesehatan sebagai pemberdayaan masyarakat agar masyarakat faham tentang Hipertensi. Selain itu, masyarakat tidak rutin melakukan pemeriksaan tekanan darah. Kegiatan pengabdian ini dilakukan bertujuan untuk mengimplementasikan program berbasis masyarakat yang memfasilitasi masyarakat dalam mebentuk kelompok masyarakat peduli Hipertensi dan menambah ilmu dan perilaku yang baik tentang penatalaksanaan Hipertensi di Desa Rajamandala Kulon Kabupaten Bandung Barat.Metodologi kegiatan pengabdian ini adalah one group pre post design dengan pelatihan kelompok peduli hipertensi pada kader dan tokoh masyarakat yang diukur pengetahuan, sikap dan self-efficacy sebelum dan seseudah pelatihan. Instrumen yang digunakan adalah pengetahuan, sikap dan self-efficacy berdasarkan penelitian sebelumnya. Analisis data menggunakan distribusi frekuensi dan Wilcoxon. Hasil kegiatan pengabdian ini adalah ada perbedaaan yang siginifikan dari pengetahuan, sikap dan self-efficacy kelompok tentang Hipertensi (p value < 0, 05). Melalui pembentukan kelompok masyarakat peduli Hipertensi berbasis masyarakat yang dapat diaplikasikan secara mandiri oleh masyarakat melalui kader kesehatan dengan pengawasan dari petugas kesehatan yang dapat berdampak pada meningkatkan derajat kesehatan masyarakat Kabupaten Bandung Barat.
TEKNOLOGI TEPAT GUNA PEMANFAATAN LISTRIK DAYA KECIL UNTUK PENGELOLAAN KANDANG PADA BUDIDAYA AYAM PEDAGING DI DESA PUCANGSONGO KABUPATEN MALANG
Authors: Parastiwi Andriani , Fauziyah Mila , Puspitasari Dwi
Published: December 2018

One of the problems faced by small-scale broiler cultivators in Pucangsongo-Malang was the use of PLN electricity that exceeds the installed capacity. Partners feel it was too expensive to raise the power capacity. To overcome the problem, timing settings to power the equipment is needed. The cage-smell’ problem needs to be handled because the cage-locations are in settlement. The purpose of this activity was helping partners to increase productivity by helping solve problems from production aspects, as well as the safety. The implementation method was providing appropriate science and technology to partners in the form of electricity utilization management equipped with a cage monitoring system. In addition, it also developed a backup power source safety system that works when the electricity goes off to keep the cage remain conducive. Prior this activity, the cage-capacity was 3300 with an average death of 200 chickens. Currently, the cage-capacity increased to 3500 chickens with deaths down to 110 chickens. With the developed and installed electric power management equipped with monitoring system of cage condition, broiler cultivators in Pucangsongo-Malang can monitor the cage’s state at any time and power shortage problem can be resolved.
PENGARUH PHYSICAL EVIDENCE TERHADAP KEPUASAN MAHASISWA PROGRAM REGULER MANDIRI FAKULTAS EKONOMI DAN BISNIS UNIVERSITAS JAMBI
Authors: Yuniarti Yenny
Published: December 2018

Fenomena yang terjadi saat ini yaitu perguruan tinggi menjadi suatu komoditas yang dibutuhkan oleh masyarakat dalam upaya peningkatan taraf hidup masyarakat. Dimana perguruan tinggi yang berkualitas dilihat secara harafiah oleh masyarakat dari sarana dan prasarana yang dimiliki serta kepuasan mahasiswa yang berada di dalam lingkungan perguruan tinggi itu sendiri. Untuk itu tujuan penelitian ini adalah untuk memperoleh gambaran mengenai physical evidence pada Program Reguler Mandiri Fakultas Ekonomi dan Bisnis Universitas Jambi dan melihat pengaruhnya terhadap kepuasan mahasiswa. Sampel yang digunakan sebanyak 100 orang dengan metode penarikan sampel melalui purposive sampling. Metode analisis yang digunakan adalah path analysis. Dan hasil penelitian menunjukan bahwa terdapat hubungan langsung dan tidak langsung antara variable dari physical evidence berupa servicecape dan otherforms of tangible communication terhadap kepuasan sebesar 64,45%.
EVALUASI ANALISA JABATAN DAN IIDENTIFIKASI KEBUTUHAN PELATIHAN UNTUK PENINGKATAN KOMPETENSI PEGAWAI
Authors: Kurniawan Dwi , Sumarni Sumarni
Published: December 2018

Penelitian ini berjudul Evaluasi Analisa Jabatan Dan Identifikasi Kebutuhan Pelatihan Untuk Peningkatan Kompetensi Pegawai Di Fakultas Ekonomi Dan Bisnis Universitas Jambi. Penelitian ini menggunakan objek penelitian yaitu para pegawai yang ada di Fakultas Ekonomi dan Bisnis Universitas Jambi baik yang berstatus Pegawai Negeri Sipil (PNS) maupun Tenaga Honorer (Non PNS). Penelitian ini menggunakan pendekatan kualitatif. Hasil penelitian ini adalah 1. Hasil yang diperoleh dalam evaluasi ini dilakukan dengan cara mengedarkan form mengenai informasi analisa jabatan dari masing-masing pegawai. Dari 26 form analisa jabatan yang disebar, hanya sebanyak 11 form yang dikembalikan. Para karyawan yang mengisi form analisa jabatan sangat terlihat belum sepenuhnya memahami mengenai tugas pokok dan fungsi yang dilimpahkan kepada mereka. Pembagian tugas yang tidak jelas akan berengaruh terhadap hasil kerja yang akan ditunjukkan oleh para pegawai tersebut., dan 2. Perlu diadakan pelatihan yang bersifat periodik baik yang bersifat on the job training maupun off the job training dengan tujuan meningkatkan keterampilan dan pengetahuan seluruh anggota
EKSISTENSI SISTEM JAMINAN RESI GUDANG DALAM PERKEMBANGAN INDUSTRI DI PROVINSI JAMBI BERDASARKAN UNDANG-UNDANG NOMOR 9 TAHUN 2011 TENTANG RESI GUDANG
Authors: Suryahartati Dwi , Arsyad Arsyad , Azwan Dicky
Published: December 2018

Hak Jaminan atas Resi Gudang adalah hak jaminan yang dibebankan pada Resi Gudang untuk pelunasan hutang, yang memberikan kedudukan untuk diutamakan bagi penerima Hak jaminan terhadap kreditur yang lain. Barang yang dimaksudkan dalam sistem resi gudang adalah benda bergerak yang apat disimpan dalam jangka waktu tertentu dan diperdagangngkan secara umum. Berdasarkan Peraturan Menteri Perdagangan No. 26/M-DAG/PER/6/2007 telah menetapkan delapan komoditas pertanian sebagai barang yang disimpan di gudang dalam penyelenggaraan sistem resi gudang. Provinsi Jambi adalah salah satu Provinsi di Indonesia yang kaya akan hasil produksi komoditi ketahanan pangan, baik dari sektor pertanian maupun perkebunan. Setidaknya Komoditi Kopi, Karet, Beras.  Tujuan penelitian ini adalah untuk menganalisis bagaimana mekanisme penjaminan dengan sistem Resi Gudang, dan kendala apa yang dihadapi dalam melaksanakan Sistem resi gudang.  Metode Penelitian ini adalah Yuridis Normatif dengan pendekatan Konseptual dan Perundang-undagan, tentunya juga mempertimbangan fakta hukum yang menyebabkan adanya issu hukum. Hasil penelitian ini adalah bahwa mekanisme sistem Resi Gudang melibatkan beberapa pihak yang terintegrasi. Kealpaan salah satu pihak menyebabkan tidak berjalannya sistem. Di Propinsi Jambi penjaminan sistem resi Gudang ini belum dapat eksis sebagaimana yang dikendaki oleh Undang-undang. Kendala yang dihadapi dalam pelaksanaan sistem Resi gudang ini di Propinsi Jambi adalah persoalan komoditi dan harganya kurang stabil karena tidak ada pengaturan secara Nasional dalam jangka yang baik,an  belum ada Perdaturan Daerah yang mengatur mengenai Resi Gudang secara di limitatif untuk bekerjanya regulasi.
ANALISIS YURIDIS PENERAPAN TATA KELOLA PEMERINTAHAN YANG BAIK SEBAGAI UPAYA PENCEGAHAN TINDAK PIDANA KORUPSI DI PEMERINTAHAN DAERAH KABUPATEN SAROLANGUN
Authors: Raharja Ivan Fauzani , Hafrida Hafrida
Published: December 2018

Good governance pada dasarnya adalah suatu konsep yang mengacu kepada proses pencapaian keputusan dan pelaksanaannya yang dapat dipertanggungjawabkan secara bersama. Sebagai suatu konsensus yang dicapai oleh pemerintah, warga negara, dan sektor swasta bagi penyelenggaraan pemerintahaan dalam suatu negara. Negara berperan memberikan pelayanan demi kesejahteraan rakyat dengan sistem peradilan yang baik dan sistem pemerintahan yang dapat dipertanggungjawaban kepada publik.Indonesia merupakan salah satu negara di dunia yang sedang berjuang dan mendambakan terciptanya good governance. Namun, keadaan saat ini menunjukkan bahwa hal tersebut masih sangat jauh dari harapan. Kepentingan politik, Korupsi, Kolusi dan Nepotisme, peradilan yang tidak adil, bekerja di luar kewenangan, dan kurangnya integritas dan transparansi adalah beberapa masalah yang membuat pemerintahan yang baik masih belum bisa tercapai. Untuk mencapai good governance dalam tata pemerintahan di Indonesia, maka prinsip-prinsip good governance hendaknya ditegakkan dalam berbagai institusi penting pemerintahan. Upaya preventif merupakan upaya pencegahan sebelum tindak pidana korupsi tersebut terjadi. Salah satu upaya pencegahan dalam tindak pidana korupsi adalah melalui upaya penerapan Good Governance. Demikian juga halnya di Pemerintah daerah Kabupaten Sarolangun tidak dapat melepaskan diri dari jeratan tindak pidana korupsi. Tindak Pidana Korupsi terjadi disemua aspek baik legislatif, eksekutif maupun sektor swasta. Untuk itu penellitian ini merupakan penelitian normatif yang akan fokus pada permasalahan penerapan asas Good Governance sebagai upaya pencegahan tindak pidana korupsi di Kabupaten Sarolangun.
PENGARUH DESENTRALISASI FISKAL, GOOD GOVERNANCE DAN STANDAR AKUNTANSI PEMERINTAHAN TERHADAP AKUNTABILITAS KEUANGAN PEMERINTAH DAERAH KOTA JAMBI
Authors: Friyani Rita
Published: December 2018

Tujuan penelitian ini adalah untuk memberikan bukti empiris bahwa desentralisasi fiskal, good governance dan penerapan standar akuntansi pemerintahan berpengaruh terhadap akuntabilitas keuangan pemerintah daerah di Kota Jambi. Penelitian ini merupakan penelitian kuantitatif dan menggunakan data kuesioner. Populasi dalam penelitian ini adalah para pegawai negeri sipil di Dinas Pengelolaan Keuangan dan Aset Daerah Kota Jambi. Sampel penelitian diambil mengguankan teknik judgement sampling. Sampel pada penelitian ini adalah staf minimal golongan IIIa, kepala seksi, kepala sub bagian, kepala bidang yang bekerja dalam pelaksanaan pengelolaan keuangan dan aset daerah dan sekretaris serta kepala dinas di Dinas Pengelolaan keuangan dan aset daerah kota Jambi. Teknik analisis yang digunakan dalam penelitian ini adalah analisis regresi linier berganda dengan uji statistik F, uji statistik t dan koefisien determinasi.
PENGGUNAAN METODE MENGAJAR OLEH GURU PAI PADA SDN NO. 142/VII PULAU ARO II KEC. PELAWAN KAB. SAROLANGUN
Authors: Muspawi Mohamad
Published: December 2018

Penelitian ini bertujuan untuk: 1). Mengetahui penggunaan metode mengajar oleh guru PAI pada SDN No. 142/VII Pulau Aro II Kec. Pelawan Kab. Sarolangun. 2). Mengetahui kendala yang dihadapi oleh guru PAI dalam penggunaan metode mengajar pada SDN No. 142/VII Pulau Aro II Kec. Pelawan Kab. Sarolangun. Penelitian ini menggunakan pendekatan kualitatif yang bertujuan untuk mengkaji permasalahan dan memperoleh makna yang lebih mendalam sesuai dengan latar penelitian.Teknik pengumpulan data dengan obervasi, dokumentasi, dan wawancara. Untuk mengecek validitas data digunakan ketekunan pengamatan, triangulasi, member check, audit trail. Dan untuk menganalisis data digunakan penelaahan dan reduksi data, unitisasi data, kategorisasi data, dan interpretasi data. Hasil penelitian ini menunjukkan bahwa: 1) Metode yang digunakan oleh guru PAI SDN No. 142/VII Pulau Aro II dalam mengajar antara lain: a. Metode Ceramah. b. Metode Tanya Jawab. c. Metode Penugasan.d. Demonstrasi. 2) Kendala bagi guru PAI SD No. 142/VII Pulau Aro II dalam penggunaan metode mengajar antara lain: a. Keterbatasan wawasan. b. Kurangnya media pendukung. c. Kurangnya mendapat pelatihan. Berdasarkan hasil penelitian tersebut, dapat disimpulkan bahwa penggunaan metode mengajar oleh guru PAI pada SDN No. 142/VII Pulau Aro II masih didominasi oleh metode ceramah, tanya jawab, penugasan, dan demonstrasi. Sedangkan kendala yang dihadapi oleh guru PAI adalah keterbatasan wawasan, media, dan pelatihan. Dengan demikian, penulis memberikan saran kepada pihak guru PAI SD No. 142/VII Pulau Aro II agar lebih kreatif dalam menggunakan metode mengajar. Kemudian kepada pihak kepala sekolah agar memberikan pelatihan mengenai metode mengajar, sehingga guru PAI memiliki wawasan yang lebih luas tentang metode mengajar. Serta melengkapi media yang dibutuhkan oleh guru PAI.