bluuebunny/crossref_metadata_2025 huggingface dataset:
Allelopathic effects of rapeseed (Brassica juncea) on invasive weed Phalaris minor Retz
Authors: Xu Gaofeng , Shan Shicai , Yun Zhang , Clements David Roy , Yun-hai Yang , Shao-song Yang , Feng-ping Zhen , Gui-mei Jin , Zhang Fu-dou , Feng Zhi-ke , Dong Li-yao
Published: February 2024

Background
Growing allelopathic crops to control invasive weeds is considered as an environmental friendly, and economically sustainable approach, understanding the reasons are essential for current and future green control of invasive weeds. Phalaris minor Retz. is one of the most troublesome invasive weeds worldwide. Our previous studies found that rapeseed(Brassica juncea)exhibited strong inhibitory effects of this weed in agroecosystems, but it is unknown whether allelopathic effects are involved in this process. In this study, we assessed the allelopathic effects of B. juncea to P. minor, then, allelochemicals were separated and identified from its leaves, further, biological activity and physiological responses by some individual compounds were investigated.
Results
Our results showed that water extracts from roots, stems, and leaves of B. juncea have strong allelopathic potential which can significantly inhibit the seedling growth of P. minor, and leaves have the strongest allelopathic inhibitory effects. Different solvents extracts from leaves water extracts of B. juncea significantly inhibited seed germination and seedling growth of P. minor at higher concentration (20 mg/mL), but no significantly inhibited at lower concentration (1.25 mg/mL), allelopathic effects of different solvents extracts were ranked in order from petroleum ether (strongest), ethyl acetate (moderate), N-butyl alcohol, and residual water (least). The petroleum ether extracts of B. juncea affected the protective oxidase activity and chlorophyll content, resulting in a significant increase of malondialdehyde (MDA) content and a significant reduced superoxide dismutase (SOD) activity and chlorophyll content of P. minor. Twenty-one components were identified by GS-MS, with proportion of 96.47% of petroleum ether extracts of B. juncea. The major components were erucic acid (EA) (19.08%), palmitic acid(PA) (14.16%), dibutyl phthalate(DP) (13.19%), ethyl palmitate(EP) (12.31%), linoleic acid(LA) (9.43%), and oleic acid(OA) (6.24%), among which components PA, EP, and LA exhibited strong inhibitory activity against P. minor.
Conclusions
Overall, these relults suggested that B. juncea has strong allelopathic inhibitory effect on P. minor, and PA, EP and LA were major compounds of petroleum ether extract of B. juncea and exhibited strong inhibitory activity against P. minor, suggested that B. juncea can be used for ecological control and developing environmentally friendly herbicides to control P. minor.
Identification of Lysine lactylation (kla) -related lncRNA signatures using XGBoost to predict prognosis and immune microenvironment in breast cancer patients
Authors: Chen Yu , Lin Feng , Li Hang , Liu Huan , Shen Jianlin , Zheng Lemin , Huang Shunyi
Published: February 2024

Breast cancer (BC) stands as a predominant global malignancy, significantly contributing to female mortality. Recently uncovered, histone lysine lactylation (kla) has assumed a crucial role in cancer progression. However, the correlation with lncRNAs remains ambiguous. Scrutinizing lncRNAs associated with Kla not only improves clinical breast cancer management but also establishes a groundwork for antitumor drug development. We procured breast tissue samples, encompassing both normal and cancerous specimens, from The Cancer Genome Atlas (TCGA) database. Utilizing Cox regression and XGBoost methods, we developed a prognostic model using identified kla-associated lncRNAs. The model's predictive efficacy underwent validation across training, testing, and the overall cohort. Functional analysis concerning kla-related lncRNAs ensued. We identified and screened 8 kla- associated lncRNAs to formulate the risk model. Pathway analysis disclosed the connection between immune-related pathways and the risk model of kla-associated lncRNAs. Significantly, the risk scores exhibited a correlation with both immune cell infiltration and immune function, indicating a clear association. Noteworthy is the observation that patients with elevated risk scores demonstrated an increased tumor mutation burden (TMB) and decreased tumor immune dysfunction and exclusion (TIDE) scores, suggesting heightened responses to immune checkpoint blockade. Our study uncovers a potential link between Kla-related lncRNAs and BC, providing innovative therapeutic guidelines for BC management.
Synthesis and gamma-ray shielding efficiency of borosilicate glasses doped with Zinc oxide: Comparative study
Authors: Saleh Abdelmoneim , Sayyed M. I. , Kumar Anjan , Mansour Fatma Elzahraa
Published: February 2024

This study examined the suitability of several glass compositions as a gamma-ray shielding substance. The compositions tested were of varying ZnO concentrations, specifically (60-x) B2O3 − 10Na2O − 15SiO2 − 5Al2O3 - (x + 10 )ZnO (where X = 5, 10, 15 and 20 mol%). Measurements were performed at energy levels of 0.6642, 1.1776, and 1.3343 MeV radiated from Cs137 and Co60 point sources along with a scintillation detector [NaI(TL)]. We investigated the critical properties related to gamma radiation shielding, determining the effective atomic number (Zeff), electron density (Nel), half-value layer (HVL), linear attenuation (µ) and mass attenuation (µm) coefficients, and mean free path (λ). Our results show that all glass compositions provide adequate protection against gamma radiation at the specified energy levels. The values of µ went up from 0.157 to 0.214 cm− 1 (0.6642 MeV), from 0.119 to 0.160 cm− 1 (1.1776 MeV), and from 0.114 to 0.151 (1.3343 MeV). For samples B1 and B4, the observed HVL values dropped from 4.41, 5.84, and 6.12 cm to 3.21, 4.31, and 4.61 cm at 0.6642, 1.1736, and 1.3343 MeV, respectively. Among the materials tested, prepared glasses show higher shielding capacity compared to regularly used glass and concrete samples. The study highlights these glass compositions' potential as practical materials that can shield gamma radiation.
Effects of on TLRs/MyD88 related pathways with infected by spring viremia of carp virus (SVCV) in Common Carp
Authors: Jiao Xue , Guo Zheng-yao , Dai Jing , Qian Ai-Dong , Li Yue-hong
Published: February 2024

Spring Viremia of Carp (SVCV) is a highly lethal viral infection that poses a significant threat to the survival and economic value of carp populations. The innate immune system serves as a robust defense mechanism against microbial intrusions. In this study, we delve into the intricate interaction between the Toll-like receptor (TLR) signaling pathway and SVCV.Our research findings unveil a noteworthy response to SVCV infection in EPC cells, characterized by an upregulation in the mRNA expression of key TLRs, namely TLR2, TLR3, TLR4, TLR7, TLR8, and TLR9. These findings strongly suggest their pivotal role in triggering the immune response against SVCV. Furthermore, our investigation delves into the therapeutic potential of ST2825, a MyD88 inhibitor, in mitigating the impact of SVCV infection. We observe that ST2825 effectively suppresses the production of the MyD88 protein, resulting in a notable reduction in mRNA expression levels of SVCV-G. This inhibition extends to the modulation of immune-related genes such as IRAK1 and IRF7, showcasing its potential as a therapeutic intervention. Notably, the ST2825-treated group demonstrates a significant decrease in the rate of cell apoptosis, underlining its potential in mitigating the detrimental effects of SVCV infection. Additionally, our study explores the mRNA expression of TRAF6, IRAK1, IRF3, and IRF7 in renal tissue. We observe a substantial increase in their expression levels in both the ST2825 and SVCV groups when compared to the control group, shedding further light on the intricate interplay within the TLR/MyD88-associated pathways during SVCV infection. In conclusion, our research enhances our understanding of how SVCV infection influences the TLR/MyD88-associated pathways. These findings provide critical insights into SVCV development and lay the groundwork for potential disease control strategies and the development of innovative therapeutic approaches.
Examining the Factors that Impact the Formation of Barite Scale in Water Injection Operations: Experimental Study and Quantification of Scale Formation
Authors: Hosny R. , Selim Hanaa , Mubarak Mahmoud. F
Published: February 2024

The accumulation of barium sulfate scale presents significant challenges in oil and gas production processes that involve water injection, leading to operational hurdles and reduced efficiency. This in-depth study investigates the complex factors influencing the formation of barium sulfate scale, aiming to quantify the extent of scale accumulation. The experimental methodology adopted in this research involves meticulous sample preparation, precise measurements of turbidity and electrical conductivity, rigorous jar testing, thorough analysis of the scale, and systematic evaluation across various experimental parameters. Through this comprehensive approach, the study aims to provide valuable insights into the compatibility of brine and the intricate composition of barium sulfate scale crystals. By examining the results and conducting a thorough analysis, the research seeks to contribute essential knowledge to the understanding of barium sulfate scale deposition, ultimately offering solutions to mitigate its impact on operational efficiency in oil and gas production processes involving water injection.
Potentiation of anti-obesity activity of berberine through its encapsulation in guar-acacia gum nanocomplexes in Wistar albino rats
Authors: Bakshi Jyoti , Lathar Prity , Verma Assim , Lather Deepika , Grewal Sapna , Dhingra Dinesh , Kumari Santosh
Published: February 2024

Berberine is a promising bioactive compound that has gained great attention against numerous diseases but its low solubility and poor systemic bioavailability hinders its clinical applicability. Therefore, this study attempted to enhance the therapeutic potential of berberine by its nanoencapsulation. Berberine loaded guar-acacia gum nanocomplexes (Ber/Gu-AGNCs) were prepared by ionic complexation method; characterized and evaluated for anti-obesity activity in high fat diet (HFD) induced obese rats. HFD was given to animals for 6 consecutive weeks. Orlistat (20 mg/kg, p.o.), berberine (10 mg/kg), Ber/Gu-AGNCs (10 and 20 mg/kg, p.o) and Gu-AGNCs (blank) were administered once a day after giving HFD for 6 weeks; and continued up to 12 weeks along with HFD. Obesity was evaluated by the measurement of morphological parameters, blood glucose, serum lipid profiles, liver enzymes and levels of oxidative stress markers. Moreover, histopathological studies of liver and adipose tissue were also carried out. The results showed that Ber/Gu-AGNCs exhibited spherical morphology and narrow size distribution. In addition, Ber/Gu-AGNCs were significantly more effective in controlling the body weight, BMI, adiposity index, liver index, blood glucose levels, serum lipids and oxidative stress levels in comparison to berberine in HFD-induced obese rats. Furthermore, histopathological examination of liver and adipose tissue revealed the anti-obesity effect of Ber/Gu-AGNCs (10 and 20 mg/kg), as indicated by decrease in hepatosteatosis and inflammation in liver tissue; and decrease in the size of adipocytes in fat depots. Thus, nanoencapsulation of berberine into gum nanocomplexes displayed better anti-obesity activity when compared to free berberine.
Extending X-reality technologies to digital twin in cultural heritage risk management：A comparative evaluation from the perspective of situation awareness
Authors: Guo Yuqing , Lu Shizhu , Shen Min , Liu Lin , Huang Wei , Yi Xiaolie , Zhang Jifa
Published: February 2024

Digital twins possess characteristics such as real-time capability, high precision, and high integration. The digital twin system for cultural heritage risk management includes comprehensive information about heritage, contextual data, and expert knowledge. Considering the role of Situational Awareness (SA), it facilitates more efficient risk assessment, analysis, and strategic decision-making for management personnel, thereby reducing damage to cultural heritage.This study extends X-reality technologies into the digital twin system for cultural heritage risk management. It simulates three sets of procedures for remote risk management of cultural heritage (2D desktop, VR, and AR) and explores the effectiveness of VR and AR in enhancing situational awareness from three dimensions of SART (SART-DAR, SART-SAR, SART-UOS). Additionally, we further analyze how the technological attributes of VR and AR (immersion and interactivity) impact the functional mechanism of situational awareness.The results indicate that, compared to the traditional 2D desktop, VR and AR have advantages in enhancing situational awareness of heritage risk. However, in AR mode, SART-DAR and SART-SAR dimensions did not show significant improvement. Furthermore, there is a significant difference in immersion's impact on the SART-DAR dimension. Regarding interactivity, although the results show differences in the impact on the SART-DAR dimension between VR and AR, no significant differences were observed.This study provides design references for building a digital twin system for cultural heritage risk management by deeply understanding the impact of X-reality technologies on the functional mechanism of cultural heritage risk situational awareness. Simultaneously, it offers insights for heritage site managers, heritage experts, and relevant stakeholders to enhance efficiency in risk perception.
COVID-19 and its impact on biodiversity in coffee farms in the Andean landscape
Authors: Medina David Coral , Fernández Germán Rivera , Rosero-García Doris , Martinez Ricardo Pereira , Galindo Leonardo Fabio
Published: February 2024

In Buesaco (BUE) and The Tablón of Gómez (TTG), farms in the Nariño Department fauna likely benefited from reduced human activities by COVID-19. However, this phenomenon has not been documented, and perhaps this article is the first on this topic. A survey was conducted on 431 farmers to identify fauna sightings and plant species. The non-parametric Kruskal Wallis test and multiple regression explored variables influencing species composition. Additionally, BUE and TTG’s fauna composition reports were utilized in a multi-temporal analysis before and after the COVID-19 lockdown. Analyzing data resulted in a compilation of lists containing the names of existing plant and fauna species. Sixty-four species of fauna were mentioned in BUE and TTG; birds accounted for 50%, and mammals accounted for 25%. Sciurus pucheranii had the highest mention frequency (MF), with 165 times by the producers in BUE and 188 times in TTG. This is the first record for the Nariño Department. According to the dominance index, four shade species within coffee plantations are considered the most dominant. Besides, five forest species around coffee are considered dominants. The Kruskal Wallis test (p = 0,013) and the regression analyses (R2 = 0,14) indicate that the null hypothesis should be rejected (p > 0.05). Species interaction between forest and fauna probably did not influence the increased biodiversity. The COVID-19 lockdown impacted how other fauna species appeared in BUE and TTG. Moreover, from 2021 to 2023, it can be determined that more species are mentioned than those recorded in years before COVID-19.
Postoperative urinary retention induced hyponatremic seizure following total knee arthroplasty with an enhanced recovery after surgery protocol: a case report and literature review
Authors: Lee Hsin-Hsin , Tsai Yuan-Hsin
Published: February 2024

Background: Although the enhanced recovery after surgery (ERAS) protocol in total knee arthroplasty (TKA) aimed at facilitating overall outcomes and reducing complication rates, the incidence of postoperative urinary retention (POUR) is still of concern.
Case presentation: A 70-year-old woman underwent TKA with an ERAS protocol for severe osteoarthritis of right knee. She developed generalized tonic-clonic seizure on post-operative day 1. The occurrence of altered consciousness initiated further investigation, revealing hyponatremia of 117 mEq/L and significant urinary retention. Following urinary catheterization, during which 1350 mL of urine was immediately drained and an additional 2550 mL over the next 8 hours, her sodium level improved to 130 mEq/L under regular saline solution supply, accompanied by a marked improvement in consciousness. Subsequently, she was discharged with a normal sodium level and complete recovery of the conscious level. The prompt resolution of neurological symptoms highlights the correlation between hyponatremia-induced seizures and POUR.
Conclusions: Hyponatremic seizure related to POUR after TKA with an ERAS protocol represents an uncommon yet potentially lethal complication. This case emphasized the importance of recognizing and addressing hyponatremic seizure as well as considering POUR as a potential cause despite the implementation of ERAS protocol. Urinary catheterization and early removal are suggested in elderly patients scheduled for TKA under spinal anesthesia.
High biological N fixation potential dominated by heterotrophic diazotrophs in alpine permafrost rivers on the Qinghai-Tibet Plateau
Authors: Xin Yuan , Gao Qun , Chen Xin , Sun Siyue , Liu Jiao , Gao Hui , Zhou Jizhong , Xia Xinghui
Published: February 2024

Background
Biological nitrogen (N) fixation is a pivotal N source in N-deficient ecosystems. The alpine permafrost rivers on the Qinghai‒Tibet Plateau (QTP), which are recognized as N-limited and sub-anoxic environments, provide a suitable habitat for diazotrophs. However, the diversity, structure, and ecological functions of diazotrophic communities in these alpine permafrost QTP rivers remain poorly understood. Here, we examined diazotrophic communities in QTP rivers via the nitrogenase (nifH) gene sequencing and assessed their N-fixing activities via a 15N isotope incubation assay. We also investigated the regulating mechanism of altitude and other geochemical factors on the diazotrophic communities and their associated N-fixing activities in QTP rivers.
Results
Strikingly, anaerobic heterotrophic diazotrophs, such as sulfate- and iron-reducing bacteria, had emerged as dominant N fixers, overshadowing the contributions of well-known phototrophic cyanobacterial diazotrophs. Remarkably, the nifH gene abundance and N fixation rates increased with altitude, and the average nifH gene abundance (2.57 ± 2.60 × 108 copies g− 1) and N fixation rate (2.29 ± 3.36 nmol N g− 1 d− 1) surpassed that documented in most aquatic environments. Such distinctive heterotrophic diazotrophic communities and high N fixation potential in QTP rivers were associated with low-nitrogen, abundant organic carbon and special C:N:P stoichiometries. Additionally, the significant presence of psychrophilic bacteria within the diazotrophic communities, along with the enhanced stability and complexity of the diazotrophic networks at higher altitudes, clearly demonstrate the adaptability of diazotrophic communities to extreme cold and high-altitude conditions in QTP rivers. We further determined that altitude, coupled with organic carbon and C:N:P stoichiometries, was the predominant driver shaping diazotrophic communities and their N-fixing activities. Based on the N fixation rates obtained in this study, the biological N fixation was estimated to be 2.79 ± 4.10 Gg N yr− 1 in QTP rivers.
Conclusions
The alpine permafrost rivers on the Qinghai-Tibet Plateau (QTP) possess distinctive heterotrophic diazotrophic communities and high biological N fixation potential, which are associated with altitude, abundant organic carbon, and special C:N:P stoichiometries. The estimated nitrogen input into the QTP rivers by biological N fixation would largely compensate for the nitrogen loss by denitrification. Overall, our study provides novel insights into nitrogen dynamics in alpine permafrost rivers.
Understanding distress in patients before breast cancer diagnosis in China：A Mixed Methods Study
Authors: Gao Yan , Wang Linying , Guo Jun , Gao Jinnan
Published: February 2024

Purpose: Although there is extensive research on the link between breast cancer diagnosis and psychological distress after diagnosis, there is limited data on the prevalence of psychological distress prior to breast cancer diagnosis. The purpose of this study is to measure the distress levels of patients before breast cancer diagnosis, to identify the categories of distress they have, and to describe the experience of distress of patients before diagnosis.
Methods: This study uses a mixed method of survey and qualitative interviews. In the quantitative part, a cross-sectional survey of 118 patients with completed breast biopsies awaiting pathological diagnosis was conducted using the National Comprehensive Cancer Network's (NCCN's) Distress Thermometer(DT) and Problem List (PL), In the qualitative part, 15 patients were invited using convenient and purposive sampling. Data was collected using a semi-structured face-to-face interview and recorded. The transcribed text was analyzed using Colaizzi's phenomenological method.
Results: The results reveal that 63.6% of patients reported a higher level of psychological distress (n=75, scoring≥4).Most patients had emotional (98%) or physical (64%) problems. Among these, worry or anxiety (47.4%), fear (33%), finances (21.1%), sadness and depression(18.6%), and grief or loss(15.2%) were the most frequently reported problems. Multivariate logistic regression analysis showed that the factors associated with psychological distress before the diagnosis of breast cancer patients were age, education level and employment. The experience of patients before diagnosis emerged four themes: Very high psychological distress index; Sources of psychological distress; The demand for information; Positive coping.
Conclusions: Psychological distress is prevalent prior to breast cancer diagnosis and may merit early intervention. Gaining insight into patients' emotional distress can assist in shaping future evaluations and interventions focused on their psychosocial needs, to enhance their overall well-being.
Impact of mechanical power on mortality in ventilated critically ill patients. Retrospective study with continuous real-life data
Authors: Manrique Sara , Ruiz-Botella Manuel , Murillo Natalia , Canelles Sandra , Victoria Ivan David , Samper Manuel Andres , Plans Oriol , Claverias Laura , Magret Monica , Gordo Federico , Roca Oriol , Bodi Maria
Published: February 2024

Background
Over the past decade, numerous studies on potential factors contributing to ventilation-induced lung injury have been carried out. Mechanical power has been pointed out as the parameter that encloses all ventilation-induced lung injury-contributing factors. However, studies conducted to date provide data regarding mechanical power during the early hours of mechanical ventilation that may not correspond to the real scenario.
Methods
Retrospective observational study conducted at a single center in Spain. Patients admitted to the intensive care unit, > o = 18 years of age, and ventilated for over 24 hours were included.
We extracted the mechanical power values throughtout the entire mechanical ventilation period from the clinical information system every two minutes. First, we calculate the cutoff-point for mechanical power beyond which there was a greater change in the probability of death. After, the sum of time values above the safe cut-off point was calculated to obtain the value in hours. We analyzed if the number of hours the patient was under ventilation with a mechanical power above the safe threshold was associated with mortality, invasive mechanical ventilation days, and intensive care unit length of stay. We repeated the analysis in different subgroups based on the degree of hypoxemia and in patients with SARS CoV-2 pneumonia.
Results
The cut-off point of mechanical power at with there is a higher increase in mortality was 18J/min. The greater the number or hours patients were under mechanical power > 18 J/min the higher the mortality in all the study population, in patients with SARS CoV-2 pneumonia and in mild to moderate hyopoxemic respiratory failure. The risk of death inceases 0.1% for each our with mechanical power exceeding 18 J/min. The number of hours with mechanical power > 18 J/min also affected the days of invasive mechanical ventilation and intensive care unit length of stay.
Conclusions
Continuous monitoring of mechanical power using an automated clinical information system shows that the number of hours with mechanical power > 18 J/min increases mortality in critically ill patients.
Evaluation of multidisciplinary high-risk pregnancy clinic for myelomeningocele
Authors: Anderson Luke , Hopson Betsy , Caudill Caroline , Rocque Brandon G. , Blount Jeffrey , Arynchyna-Smith Anastasia , Thrower Jessica , Johnston James , Rozzelle Curtis
Published: February 2024

A cross-sectional study retrospectively evaluating the perceived usefulness of attending a multi-disciplinary, roundtable, educational prenatal clinic for mothers expecting children with myelomeningocele is presented. Mothers who currently have children with SB completed a survey which evaluated their overall preparedness, spina bifida education, delivery plans, surgical expectations, and expectations in terms of quality of life and development. Open comments were also collected. Approximately half of these mothers received some form of prenatal SB counseling. Statistical analysis was performed to identify differences between those who attended prenatal counseling and those who did not. Mothers who attended prenatal counseling reported that they felt more informed and prepared throughout their pregnancy, during the delivery of their child, and during their initial hospital stay than mothers who did not. They reported that the roundtable discussions were beneficial, and the education they received was useful in helping them form accurate expectations and feel more at ease. This suggests that prenatal counseling and the High-Risk Pregnancy Clinic (HRPC) provides perceived utility to families and mothers and that the HRPC is an effective method of providing prenatal counseling to mothers whose unborn children have been diagnosed with myelomeningocele.
Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression
Authors: Li Lixia , Liu Dewei , Chen Tingting , Wei Chunhui , Qiao Youping , Liu Weiliang , Liang Yanmei , Liang Zhu , Chen Chunyuan , Li Dongming , Wu Bin , Zhao Xuanna , Huang Dan , Wu Dong
Published: February 2024

Background
The progression of non-small cell lung cancer (NSCLC) is significantly influenced by circular RNAs (circRNAs), especially in tumor hypoxia microenvironment. However, the precise functions and underlying mechanisms of dysregulated circRNAs in NSCLC remain largely unexplored.
Methods
Differentially expressed circRNAs in NSCLC tissues were identified through high-throughput RNA sequencing. The characteristics of circ_0007386 were rigorously confirmed via Sanger sequencing, RNase R treatment and actinomycin D treatment. The effects of circ_0007386 on proliferation and apoptosis were investigated using CCK8, cloning formation assays, TUNEL staining, and flow cytometry assays in vitro. In vivo, xenograft tumor models were used to evaluate its impact on proliferation. Mechanistically, the regulatory relationships of circ_0007386, miR-383-5p and CIRBP were examined through dual luciferase reporter assays and rescue experiments. Additionally, we detected the binding of EIF4A3 to CRIM1 pre-mRNA by RNA immunoprecipitation and the interaction between YAP1 and EIF4A3 under hypoxic conditions by co-immunoprecipitation.
Results
Our investigation revealed a novel circRNA, designated as circ_0007386, that was upregulated in NSCLC tissues and cell lines. Circ_0007386 modulated proliferation and apoptosis in NSCLC both in vitro and in vivo. Functionally, circ_0007386 acted as a sponge for miR-383-5p, targeting CIRBP, which influenced NSCLC cell proliferation and apoptosis via the PI3K/AKT signaling pathway. Furthermore, under hypoxic conditions, the interaction between YAP1 and EIF4A3 was enhanced, leading to the displacement of EIF4A4 from binding to CRIM1 pre-mRNA. This facilitated the back-splicing of CRIM1 pre-mRNA, increasing the formation of circ_0007386. The circ_0007386/miR-383-5p/CIRBP axis was significantly associated with the clinical features and prognosis of NSCLC patients.
Conclusions
Circ_0007386, regulated by YAP1-EIF4A3 interaction under hypoxia conditions, plays an oncogenic role in NSCLC progression via the miR-383-5p/CIRBP axis.
Local Delivery of Accutox® Synergises With Immune-checkpoint Inhibitors at Disrupting Tumor Growth
Authors: Bikoriman Jean-Pierre , Hachme Nehme , Abusara Jamilah , Gonçalves Marina Pereira , Farah Roudy , Talbot Sebastien , Beaudoin Simon , Stanga Daniela , Plouffe Sebastien , Rafei Moutih
Published: February 2024

Background: The Accum® platform was initially designed to accumulate biomedicines in target cells by inducing endosomal-to-cytosol escape. Interestingly however, the use of unconjugated Accum® was observed to trigger cell death in a variety of cancer cell lines; a property that was further exploited in the development of Accum®-based anti-cancer therapies. Despite the impressive pro-killing abilities of the parental molecule, some cancer cell lines exhibited resistance. This prompt us to test additional Accum® variants, which led to the identification of the AccuTOX® molecule.
Methods: A series of flow-cytometry and cell-based assays were used to assess the pro-killing properties of AccuTOX® along with its ability to trigger the production of reactive oxygen species (ROS), endosomal breaks and antigen presentation. RNA-seq was also conducted to pinpoint the most prominent processes modulated by AccuTOX® treatment in EL4 T-cell lymphoma. Finally, the therapeutic potency of intratumorally-injected AccuTOX® was evaluated in three different solid tumors models (EL4, E0771 and B16) both as a monotherapy or in combination with three immune-checkpoint inhibitors (ICI).
Results: In total, 7 Accum® variants were screened for their ability to induce complete cell death in 3 murine (EL4, B16 and E0771) and 3 human (MBA-MD-468, A549, and H460) cancer cell lines of different origins. The selected compound (hereafter refereed to as AccuTOX®) not only displayed an improved killing efficiency (~5.5 fold compared to the parental Accum®), but it did so while retaining its ability to trigger immunogenic cell death, ROS production, and endosomal breaks. Moreover, transcriptomic analysis revealed that low dose AccuTOX® enhances H2-Kb cell surface expression as well as antigen presentation in cancer cells. The net outcome culminates in impaired T-cell lymphoma, breast cancer and melanoma growth in vivo especially when combined with anti-CD47, anti-CTLA-4 or anti-PD-1 depending on the animal model.
Conclusions: AccuTOX® exhibits enhanced cancer killing properties, retains all the innate characteristics displayed by the parental Accum® molecule, and synergizes with various ICI in controlling tumor growth. These observations will certainly pave the path to continue the clinical development of this lead compound against multiple solid tumor indications.
Factors influencing the physio psychosocial state and health concerns of Canadian and Iranian undergraduate nursing students during the pandemic
Authors: Okuyan Canan Birimoglu , Begen Mehmet , Mohtashami Jamileh , Güngörmüş Zeynep
Published: February 2024

The aim of our study is to investigate and analyze the factors that influence the physical, psychological, and social well-being of nursing students in Canada and Iran amidst the COVID-19 pandemic.The current study is both descriptive and comparative. Nursing students from both Canadian and Iranian institutions. Data was collected using a questionnaire based on the Short Health Anxiety Inventory (SHAI) scale and the Physio-Psychosocial Response Scale (PPSRS).
There were statistically significant positive and extremely strong correlations (respectively; r=.911, r=.964, r=.952). between the total score of the physio-psycho-social response scale and the social, emotional, and somatic subscales of the students' subgroups. According to our findings, the COVID-19 pandemic has had a negative impact on the physio-psycho-social well-being of Canadian and Iranian nursing students. The total scores of the physio psychosocial response scale and the health anxiety scale were found to have a statistically significant positive relationship.The research findings support that during the pandemic, psychological support initiatives for nursing students should be prioritized. Moreover, the present study could be valuable in assessing the immediate psychological needs of the general population who are encountering physical symptoms during the epidemic.
Efficient Server Room Monitoring with Flutterand Firebase - Real-Time Insights and InstantNotifications
Authors: Özkurt Cem
Published: February 2024

A comprehensive server room monitoring system is presented in this research,leveraging Raspberry Pi devices, DHT11 temperature/humidity sensors, PIRmotion sensors, and magnetic reed switches. Integration of Flutter and Firebasetechnologies enabled real-time environmental monitoring, security event detection, and remote access control. The effectiveness of Raspberry Pi devices infacilitating cost-effective and scalable monitoring solutions was demonstrated,while Flutter and Firebase enhanced accessibility, usability, and responsiveness.The aim of this research is to improve server room management, security, andoperational efficiency, contributing to a more secure and resilient infrastructurein the digital age
Global research trends on sepsis and septic shock during 2003–2022: A data-driven bibliometric analysis
Authors: Han Weiying , Guo Haoxin , Wang Zhongqing , Ding Renyu
Published: February 2024

Background
Sepsis is a major medical condition that contributes to substantial morbidity and mortality rates worldwide. Research to better understand and manage these complex conditions has intensified over the past two decades. This bibliometric analysis aims to map global research trends in sepsis and septic shock from 2003 to 2022, providing insights into the evolution of the field.
Methods
This study conducted a comprehensive search for publications on sepsis and septic shock using the Web of Science Core Collection. Data were analysed using VOSviewer for bibliometric mapping, focusing on publication trends, country, institutional contributions, journal sources, citation analysis, and keyword co-occurrence.
Results
In total, 78,108 publications were retrieved, with a notable increase in annual output over the study period. The United States ranked first in publication volume, followed by China, Germany, and the United Kingdom. Harvard University was the most prolific institution. Critical Care Medicine emerged as the leading journal in terms of publication count. The most cited articles and references were predominantly related to definition, epidemiology, and management guidelines for sepsis. Recent research hotspots included COVID-19, machine learning applications, NLRP3 inflammasome, autophagy, gut microbiota, and microRNA (miRNA).
Conclusions
Our bibliometric analysis identifies global research trends in sepsis over the past 20 years, with evolving hotspots and increasing global collaboration. Key findings highlight continuous efforts to understand the complex pathophysiology of sepsis and septic shock. COVID-19, machine learning applications, NLRP3 inflammasome, autophagy, gut microbiota, and microRNA (miRNA) are hotspots.
Diffusely adherent and cyclomodulin- positive Escherichia coli are associated with colon diseases
Authors: Hosseininasab Seyed_Abolfazl , Saffari Fereshteh , Tadjrobehkar Omid , Zandi Hengameh , Ahmadi Bijan , Khoshnood Asghar , Niksefat Naser , Mehboodi Mahtab
Published: February 2024

Any alteration in gut microbiome may result in colonization of some pathobionts and consequently some colon diseases. The present study was performed to determine association between E.coli pathobionts (cyclomodulin positive and afa-C + diffusely adherent E.coli) and also some characteristics of them in patients with colon diseases. Stool specimens were obtained from patients referred to colonoscopy centers of university hospitals of Yazd and Kerman, Iran. Totally 67 patients voluntarily joined the study as target group (21 case of colorectal cancer and 46 case of inflammatory bowel disease) as well as 67 healthy individuals. Stool samples were screened for Escherichia coli isolates by culture technique. Cyclomodulin encoding genes (clbN, cnf, cdt and cif) as well as afa-C, were tracked by PCR assay. Phylogrouping, virulence gene screening, antibiotic susceptibility evaluation and biofilm formation assessment were also performed. Results showed clbN, cnf and afa-C + DAEC were significantly associated with CRC. The latter was also associated with IBD (p < 0.05). Most of the isolates from patients group, belonged to B2 phylogroup. Iron uptake related genes were significantly associated with patients. Antibiotic resistance and biofilm formation were not significantly associated with any studied groups. In conclusion, this study provides primarily data about the status of some important E.coli pathobionts involved in colon diseases. It appears that afa-C + DAEC was more associated with colon diseases so it could be proposed as a putative marker for screening procedures. However, as few of specimens were positive for afa-C and cyclomodulins (clb and cnf), definitive conclusion require more comprehensive investigation.
Provider and community perspectives of integrated COVID-19 and routine childhood immunisation programmes in Nigeria: a qualitative exploratory study
Authors: Bakare Ayobami A. , Akinsola Kofoworola O. , King Carina , Sogbesan Abiodun A. , Bakare Oluwabunmi R. , Fadahunsi Opeyemi Y. , Salako Julius , Falade Adegoke G. , Wees Sibylle Herzig
Published: February 2024

Background
In Nigeria, COVID-19 vaccines were delivered through outreach activities and integrated with routine immunisation. However, evaluations of integrated approaches for novel vaccines are scarce. We aimed to understand the perceived benefits and challenges of integrating the COVID-19 vaccine within routine- immunisation in Nigeria and identify ways to strengthen this approach.
Methods
We conducted 30 semi-structured interviews with community members and healthcare workers in primary healthcare (PHC) facilities in Jigawa (n = 16) and Oyo (n = 14) states, Nigeria in August 2022. Participants were selected purposively from PHC facilities. Healthcare worker and community interviews were analysed separately using thematic analysis.
Results
We identified four themes that describe the community response, perceived impact, and health system adaptions to the challenges associated with the integrated vaccine delivery approach. Community members expressed concern that children might be given COVID-19 vaccines instead of routine immunisations, while others appreciated the integrated approach due to their trust in the efficacy of COVID-19 vaccines, government, and healthcare providers. Healthcare providers perceived the integrated approach as improving vaccination coverage and awareness but noted additional problems of increased workload, vaccine scarcity, and prolonged clinic visits. Insufficient resources were subsisting barriers to effective integration in both states, but the provider’s gender was also a challenge in Jigawa state. Additionally, the use of incentives to generate demand had ambiguous effects in Jigawa state.
Conclusion
Taking an integrated approach to deliver COVID-19 vaccines was acceptable by health workers but resisted from the community. Addressing persistent challenges in existing vaccination programmes is pertinent to enhance effectiveness and acceptability of the integrated approach.
Molecular profiling of Rosa canina L. Greek native germplasm collection for enhanced fruit extract production: A comprehensive approach utilizing neutral, gene, and exon-based markers
Authors: Boutsika Anastasia , Mellidou Ifigeneia , Grigoriadou Katerina , Papapanastasi Katerina , Krigas Nikos , Maloupa Eleni , Ganopoulos Ioannis , Xanthopoulou Aliki
Published: February 2024

The genus Rosa L. is globally distributed and encompasses the economically and ecologically important species Rosa canina L. Apart from the traditional uses of R. canina in folk medicine, food, cosmetics, and ornamental applications, it is renowned for the functional bioactive components found in rose hips. Identifying the genetic diversity within this species is crucial for any plant breeding project. This study employed three molecular markers (ISSR, SCoT, and EBAP) to conduct the first comprehensive genetic analysis of 12 R. canina genotypes. DNA extraction, marker selection, and PCR amplification were performed following established protocols. The resulting genetic data were analyzed for polymorphism, diversity indices, and population structure using various statistical methods, including PCA, UPGMA clustering, and STRUCTURE analysis. The ISSR analysis revealed a high level of polymorphism (81.82%) and identified two major clusters in the UPGMA dendrogram. SCoT and EBAP markers also exhibited substantial polymorphism (74.56% and 82.11%, respectively) and formed three distinct clusters. PCA indicated a consistent pattern across markers, suggesting reliable genetic grouping. STRUCTURE analysis supported the presence of three genetically uniform subpopulations (K = 3) within the studied R. canina germplasm collection. This study provides a comprehensive genetic characterization of the Greek native R. canina gene bank collection. The observed genetic diversity and population structure provided valuable insights for future breeding programs targeting specific genetic clusters within R. canina populations.
Behaviors of nucleosomes with mutant histone H4s in euchromatic domains of living human cells
Authors: Semeigazin Adilgazy , Iida Shiori , Minami Katsuhiko , Tamura Sachiko , Ide Satoru , Higashi Koichi , Toyoda Atsushi , Kurokawa Ken , Maeshima Kazuhiro
Published: February 2024

Since Robert Feulgen first stained DNA in the cell, visualizing genome chromatin has been a central issue in cell biology to uncover how chromatin is organized and behaves in the cell. To approach this issue, we have developed single-molecule imaging of nucleosomes, a basic unit of chromatin, to unveil local nucleosome behavior in living cells. In this study, we investigated behaviors of nucleosomes with various histone H4 mutants in living HeLa cells to address the role of H4 tail acetylation, including H4K16Ac and others, which are generally associated with more transcriptionally active chromatin regions. We ectopically expressed wild-type (wt) or mutated H4s (H4K16 point, H4K5,8,12,16 quadruple, and H4 tail deletion) fused with HaloTag in HeLa cells. Expressed wtH4-Halo, H4K16-Halo mutants, and multiple H4-Halo mutants had the euchromatin-concentrated distribution. Consistently, the genomic regions of the wtH4-Halo nucleosomes corresponded to Hi-C contact domains with active chromatin marks (A-compartment). Utilizing single-nucleosome imaging, we found that none of the H4 deacetylation or acetylation mimicked H4 mutants altered the overall local nucleosome motion. This finding suggests that H4 mutant nucleosomes embedded in the condensed euchromatic domains with excess endogenous H4 nucleosomes cannot cause an observable change in the local motion. Interestingly, H4 with four lysine-to-arginine mutations displayed a substantial freely diffusing fraction in the nucleoplasm, whereas H4 with a truncated N-terminal tail was incorporated in heterochromatic regions as well as euchromatin. Our study indicates the power of single-nucleosome imaging to understand individual histone/nucleosome behavior reflecting chromatin environments in living cells.
Exploring the Potential Link between Vitamin D Receptor Cdx2 Gene Polymorphisms and Rheumatoid Arthritis in Female Patients: A Study in Erbil, Iraq
Authors: Qadir Raghda R. , Shnawa Bushra H. , Ahmed Mukhtar H.
Published: February 2024

Background:In the last decades, vitamin D has been a subject of research in several autoimmune diseases, including Rheumatoid Arthritis (RA), a chronic inflammatory disease characterized by a distinctive pattern of joint involvement and joint destruction.Aim: The main goal of the study is to examine the relationship between (RA) and the polymorphism in the vitamin D receptor's Cdx2 gene in a sample of women.Methods:Eighty females (60 with RA and 20 controls) participated in this case-control investigation. The Cdx2 single nucleotide polymorphisms (SNP) of the VDR gene were genotyped using tetra-primer amplification refractory mutation system-polymerase chain reaction technique (ARMS-PCR).Results:The current study discovered that 51 RA patients have the heterozygous GA genotype of the Cdx2 polymorphism despite non-significant values. Although the present findings showed no significant association between Cdx2 gene polymorphisms and the risk of RA, this genotype still represents the majority among the study's RA patients. Moreover, the results showed that the serum VD3 was significantly lower in RA with the GA genotype compared to healthy controls with a similar genotype (p=0.0002). Also RA patients with heterozygote GA genotype for Cdx2 had a relatively lower mean for serum vitamin D3 level than those with homozygote GG genotype.Conclusion:The novelty of this study is to discover the probable association of Vitamin D receptor, caudal-related homeobox 2 (VDR-Cdx2) polymorphism with RA in the given study group for the first time in our society using a tetra primer ARMS-PCR. Additionally, the tetra-primer ARMS-PCR technique was simple, reliable, low-cost, and might be a valuable substitute for Restriction fragment of length polymorphism- polymerase chain reaction– (RFLP-PCR) in routine clinical settings for single nucleotide polymorphism (SNP) genotyping.
Synthesis of Ag-Doped CuPc-ZnO/Hydrophobic Silica Aerogel Photocatalyst for Enhanced Photocatalytic Degradation of Floating Benzene: A Comparative Study of Different Photoreactor Geometries
Authors: Hakki Hamid Kazemi , Seyyedbagheri Hadi , Ahmed Shahla Zubair , Avalzali Hossein Alinezhad , Ghaderi Aref
Published: February 2024

Water pollution caused by water-immiscible pollutants such as BTXs has become a major concern for researchers in recent years. This study presents the synthesis of a hydrophobic photocatalyst, Ag-CuPc-ZnO/Silica Aerogel, prepared using sol-gel and impregnation methods for the degradation of floating benzene. The effect of different percentages of Ag on the photocatalyst performance was studied, and the optimal sample was tested in rectangular and cylindrical photoreactors. The physicochemical properties of the hydrophobic photocatalyst were analyzed using XRD, FESEM, FTIR, BET/BJH, PL, contact angle, and UV-Vis DRS. Our analysis showed that decreasing nanoparticle size led to an increased specific surface area and decreased pore diameter. DRS analysis demonstrated that increasing Ag content led to a decreased bandgap and increased light absorption in the visible light region with CuPc addition. PL analysis indicated a significant decrease in electron-hole recombination with 5% Ag and confirms the efficient charge separation. The 5% wt. Ag sample achieved highest benzene photodegradation efficiency, while the rectangular photoreactor outperformed with superior degradation rates, STY, PSTY, and QY due to uniform light distribution and improved illumination. This study offers valuable insights on hydrophobic photocatalysts for degrading water-immiscible pollutants, emphasizing the need to optimize catalyst performance for efficient wastewater treatment.