bluuebunny/crossref_metadata_2025 huggingface dataset:
Adrenomedullin, transcriptionally regulated by vitamin D receptors, alleviates atherosclerosis in mice through suppressing AMPK‐mediated endothelial ferroptosis
Authors: Hu Yanchao , Gu Xu , Zhang Yan , Ma Weidong , Sun Lijun , Wang Congxia , Ren Bincheng
Published: September 2023

Purpose

Vitamin D receptors (VDR) play important roles in cardiovascular, immune, metabolic and other functions. Activation of VDR may help improve endothelial dysfunction, atherosclerosis, vascular calcification, and cardiac hypertrophy. However, the specific target genes and mechanisms of VDR in improving Human Umbilical Vein Endothelial Cell (HUVEC) functions remain unclear. This study aims to investigate the function and mechanism of VDR in HUVECs.

Methods

Endothelial dysfunction cell model was constructed by oxidized low‐density lipoprotein (ox‐LDL). An animal model of atherosclerosis was established in male homozygous Apoe−/− mice (6 weeks) on a high fat diet for 6 weeks. The relationship between VDR and adrenomedullin (ADM) was studied by bioinformatics analysis, ChIP, and luciferase reporter gene analysis. Endothelial cell function was evaluated by Transwell migration and Tube Formation tests. Ferroptosis was detected by measuring intracellular iron content, levels of oxidative stress markers, and ferroptosis related proteins.

Results

Overexpression of VDR in HUVECs inhibits ox‐LDL‐induced endothelial dysfunction and ferroptosis. VDR binds to the ADM promoter sequence and regulates the transcription of ADM. Inhibition of ADM promotes ox‐LDL‐induced endothelial dysfunction and ferroptosis. ADM regulates ox‐LDL‐induced endothelial dysfunction and ferroptosis through the AMPK signaling pathway. Overexpression of VDR in Apoe−/− mice inhibited lipid deposition and plaque area in atherosclerotic mice.

Conclusion

VDR inhibits ox‐LDL‐induced endothelial dysfunction and ferroptosis by regulating ADM transcription and acting on AMPK signaling pathway. Overexpression of VDR in Apoe−/− mice reduced lipid deposition and plaque area in the thoracic aorta of atherosclerotic mice.
Inulin supplementation increases the differential metabolites and metabolic pathway in Baird's tapirs (Tapirus bairdii)
Authors: Liu He , Liu Yan , Hao Feier , Li Bo , Cong Yipeng
Published: September 2023

Objective

The prevalence of prebiotic has increased substantially over the past decades. Little is known on its metabolic effects in zoo herbivores. We investigated the difference in faecal metabolites to characterize the composition and pathways involved after feeding inulin in zoo Baird's tapirs (Tapirus bairdii).

Methods

Faecal samples were collected from before inulin treatment group and after treatment groups in six adult tapirs and analysed using untargeted liquid chromatography–mass spectrometry methods. The differential metabolites identified and metabolic pathways involved were analysed using KEGG annotation.

Results

The results demonstrated significant alterations in faecal metabolites and metabolic pathways in comparison to the control group. The amounts of differential metabolites and metabolic pathways tended to enrich with time after the treatment. We found that tryptophan and purine metabolism were relevant to the important metabolic pathways of the metabolite differences.

Conclusions

The findings suggest that inulin may have potential applications in captive wildlife, which may provide insights into the effects of prebiotic supplementation on gut metabolites and highlights further research in this field.
Red Fluorescent Eu(TTA)3BIP Complex and Its Application in Blue Light‐Reducing Film
Authors: Wang Zhongzhi , Shi Rui , Hao Pengcheng , Qiao Xin , Song Senchuan
Published: September 2023

In this work, a Eu(TTA)3BIP complex with a novel structure was synthesized and characterized. The Eu(TTA)3BIP complex has a wide excitation band and can be excited by light in the ultraviolet and blue regions. The characteristic red light of Eu(III) is emitted when the complex is excited. The use of the 2‐(Pyrimidin‐2‐yl)‐1H‐benzo[d]imidazole (BIP) ligand improved the UV resistance of the complex. Moreover, the blue light reducing film prepared by the Eu(TTA)3BIP complex can effectively weaken blue light irradiation. In addition, the Eu(TTA)3BIP complex exhibits fluorescence thermal quenching properties. This provides the possibility for temperature sensing.
Linear seesaw mechanism from dark sector
Authors: Hernández A. E. Cárcamo , Vishnudath K. N. , Valle José W. F.
Published: September 2023

Abstract

We propose a minimal model where a dark sector seeds neutrino mass generation radiatively within the linear seesaw mechanism. Neutrino masses are calculable, since tree-level contributions are forbidden by symmetry. They arise from spontaneous lepton number violation by a small Higgs triplet vacuum expectation value. Lepton flavour violating processes e.g. μ → eγ can be sizeable, despite the tiny neutrino masses. We comment also on dark-matter and collider implications.
Wilson-loop one-point functions in ABJM theory
Authors: Jiang Yunfeng , Wu Jun-Bao , Yang Peihe
Published: September 2023

Abstract

In this paper we initiate the study of correlation functions of a single trace operator and a circular supersymmetric Wilson loop in ABJM theory. The single trace operator is in the scalar sector and is an eigenstate of the planar two-loop dilatation operator. The Wilson loop is in the fundamental representation of the gauge group or a suitable (super-)group. Such correlation functions at tree level can be written as an overlap of the Bethe state corresponding to the single trace operator and a boundary state which corresponds to the Wilson loop. There are various type of supersymmetric Wilson loops in ABJM theory. We show that some of them correspond to tree-level integrable boundary states while some are not. For the tree-level integrable ones, we prove their integrability and obtain analytic formula for the overlaps. For the non-integrable ones, we give examples of non-vanishing overlaps for Bethe states which violate selection rules.
A lattice model for condensation in Levin-Wen systems
Authors: Christian Jessica , Green David , Huston Peter , Penneys David
Published: September 2023

Abstract

Levin-Wen string-net models provide a construction of (2+1)D topologically ordered phases of matter with anyonic localized excitations described by the Drinfeld center of a unitary fusion category. Anyon condensation is a mechanism for phase transitions between (2+1)D topologically ordered phases. We construct an extension of Levin-Wen models in which tuning a parameter implements anyon condensation. We also describe the classification of anyons in Levin-Wen models via representation theory of the tube algebra, and use a variant of the tube algebra to classify low-energy localized excitations in the condensed phase.
Minimizing the DILI potential of carboxylic acid-containing drugs: a perspective
Authors: Weidolf Lars , Wilson Ian
Published: September 2023

Drugs containing carboxylic acid moieties are associated with a range of toxicities, some related directly to their pharmacology and others to the metabolites that they produce on undergoing biotransformations that reduce biological activity and enhance excretion. The reactions involved in metabolism include a wide range of conjugations to the carboxylic acid moiety plus modifications to the spacers used to attach them to the main body of the drug. Here we provide a metabolic perspective on the biotransformations that have been found to occur with drugs that contain a carboxylic acid and discuss the potential of these to cause toxicity. Based on our current understanding of the metabolism of carboxylic acid-containing drugs we then consider approaches that may mitigate toxicity.
Subnetwork constraints for tighter upper bounds and exact solution of the clique partitioning problem
Authors: Belyi Alexander , Sobolevsky Stanislav , Kurbatski Alexander , Ratti Carlo
Published: September 2023

We consider a variant of the clustering problem for a complete weighted graph. The aim is to partition the nodes into clusters maximizing the sum of the edge weights within the clusters. This problem is known as the clique partitioning problem, being NP-hard in the general case of having edge weights of different signs. We propose a new method of estimating an upper bound of the objective function that we combine with the classical branch-and-bound technique to find the exact solution. We evaluate our approach on a broad range of random graphs and real-world networks. The proposed approach provided tighter upper bounds and achieved significant convergence speed improvements compared to known alternative methods.
Clinical and radiological evaluation of radial neck factures in children and adolescents treated by percutaneous leverage reduction with Kirschner wire stabilization
Authors: Guzikiewicz Norbert , Małecki Krzysztof , Sibiński Marcin , Niedzielski Kryspin
Published: September 2023

Purpose

The aim of the study was to evaluate the clinical and radiological results of surgical treatment of radial neck fractures in children and adolescents by percutaneous leverage with Kirschner wire stabilization.

Methods

A retrospective clinical and radiographical evaluation was performed on a cohort of 61 patients (mean age 9.7 years; range 3 to 15) with isolated, unilateral radial neck fractures treated between 2009 and 2019. The mean duration of follow-up was 4.2 years (range 2 to 9 years). All fractures were types III and IV according to Judet’s classification.

Results

After mean follow-up, the radiographic results according to Metaizeau were rated as excellent in 70.5% of respondents, good in 27.9%, satisfactory in 1.6%. According to Mayo Elbow Performance Score, 95.1% of respondents obtained a very good result, 3.3% good, and 1.6% satisfactory. The mean radial neck–shaft angle changed from a mean 51.5° before operation to 3.8° postoperatively (p<0.001). The mean translation was 3.1mm before surgery and 0.5mm postoperatively (p<0.001). No limb axis deviation, elbow joint instability, and infection of the implant insertion site were observed. No statistically significant differences were noted between girls and boys (p>0.05).

Conclusions

Our findings indicate that percutaneous leverage with Kirschner wire stabilization is an effective and safe method for treating isolated radial neck fractures, characterized by a low risk of iatrogenic complications.
Taking Stock of Knowledge Transfer Studies: Finding Ways Forward
Authors: Lundmark Carina , Nilsson Jens , Krook-Riekkola Anna
Published: September 2023

Knowledge transfer (KT) from academia to practice is important in many fields, but comprehensive studies on identifying the most effective forms of KT are scarce. This paper aims to provide an overview of KT theory and presents a cross-disciplinary scoping review of empirically oriented peer-reviewed articles. The review offers guidance for researchers seeking to communicate effectively with practitioners. It explores the effects of research communications, delves into the understanding and measurement of these effects, attempts to identify the most effective forms of communication, and highlights important considerations when designing KT strategies. Few studies in our sample (eight of 27) systematically measured effects of KT, and merely four studies compared multiple forms of KT. Nevertheless, most studies estimated effects from KT, regardless of the chosen form (e.g., workshops or lectures). Most studies estimated knowledge change as the primary outcome. Additionally, several studies explored altered beliefs such as increased self-efficacy. A third of the studies addressed how the knowledge was applied, ranging from sharing information to developing new habits. The identified effects were, however, both small and volatile. Our findings underscore the significance of continuity and repeated interactions to enhance the impact of KT initiatives. Furthermore, researchers need to develop a comprehensive set of tools to facilitate successful KT, considering factors such as expertise, communication skills, trust-building, and participant-centered approaches. By employing these strategies, researchers can effectively bridge the gap between academia and practice, facilitating successful KT in various fields.
Quantifying endolithic bioerosion rates on remote coral reefs in the Central Indian Ocean
Authors: Lloyd Newman Jake E. , Perry Chris T. , Lange Ines D.
Published: September 2023

Bioerosion of calcium carbonate is a fundamental process that impacts net coral reef accretion. Besides large grazers, endolithic organisms play a major role in carbonate removal. Here we provide the first rate data for both macro- and microendolithic bioerosion in the remote Chagos Archipelago, Indian Ocean. Based on analysis of experimental blocks using computer tomography, we show similar macrobioerosion rates at 5 m (0.086 ± 0.026 kg m−2 yr−1) compared to at 10 m depth (0.066 ± 0.016 kg m−2 yr−1) after three years of exposure, with a succession from worm to sponge bioeroders over time. Microbioerosion rates analysed with scanning electron microscopy were 2–5 × higher than macrobioerosion rates at 5 m (0.187 ± 0.028 kg m−2 yr−1) and 10 m depth (0.313 ± 0.049 kg m−2 yr−1), but the microborer community was dominated by cyanobacteria in all samples. Total endolithic erosion was small compared to external erosion by parrotfishes, which increased over time, but did not show significant differences between 5 m (0.74 ± 0.11) and 10 m depth (1.12 ± 0.16 kg m−2 yr−1) after three years of exposure. These erosion rates are indicative of the oligotrophic and remote reef setting in the Chagos Archipelago (clear water, low nutrients, high fish biomass). The data will help to improve local carbonate budget estimates and provide a context for wider regional and environmental comparisons.
Spina bifida and cardiorespiratory profile: the impact of leisure sport activities on physical fitness
Authors: Monti Riccardo , Mariani Francesco , Mastricci Rosanna , Nifosì Francesco Maria , Palmieri Vincenzo , Manes Gravina Ester , Capriati Margherita , Rendeli Claudia
Published: September 2023

Purpose

The aim of the present study is to evaluate a population of young patients affected by Spina Bifida (SB) to describe their cardiorespiratory function and bone mineral density profile, analyzing any differences between people performing and those who do not perform sports activity. The study also aimed to rule out possible congenital heart disease associated with spina bifida, considering the common origin of certain cardiac structures with those found to be altered in SB patients.

Methods

Thirty-four young patients, aged between 12 and 22 years, diagnosed with spinal dysraphism (SD), have been clinically described and, in order to evaluate their physical fitness, functional capacity and bone mass, almost all of them underwent a complete cardiorespiratory assessment, including electrocardiogram (ECG), echocardiogram, Cardiopulmonary Exercise Test (CPET), body composition analysis using bioimpedance analysis (BIA) and Dual Energy X-ray Absorptiometry (DEXA), as well as the estimation of bone mineral density (BMD) with Computerized Bone Mineralometry (CBM).

Results

Collected data demonstrated that only 35% of the subjects practiced physical activity during the week. BMI and percentage FM values were pathological in at least 50% of the population. On cardiological investigations (ECG and echocardiogram), no significant alterations were found. In all patients who performed CPET (79.4%), pathological values of the main functional capacity parameters were revealed, especially peak oxygen consumption (VO2 peak), even when corrected for BCM or FFM estimated at BIA and DEXA, respectively. In the CBM analysis, out of 27 patients in whom the femoral T-score was evaluated, a condition of osteopenia was revealed in 40.7% of the patients (11/27) and osteoporosis in 18.5% (5/27); out of 27 patients in whom the lumbar T-score was evaluated, 37% of the patients showed osteopenia (10/27) and 29.6% osteoporosis (8/27). When the comparison between exercising and non-exercising patients was performed, the only statistically significant difference that emerged was the median lumbar T-score value, which appeared lower in the group not performing physical activity (p = 0,009).

Conclusions

The extensive cardiorespiratory evaluation, including CPET, of our cohort of spina bifida patients showed altered values of the main parameters related to cardiorespiratory fitness and is the only study in the literature that analysed bone mineralization values in physically active and sedentary spina bifida patients and demonstrated a statistically significant difference. Furthermore, it is the only study to date that investigated the possible association of congenital heart diseases with SD, without demonstrating the existence of pathological conditions.
SLA2 is a prognostic marker in HNSCC and correlates with immune cell infiltration in the tumor microenvironment
Authors: Wu Zhongbiao , You Chengkun , Zhu Zhongyan , Wu Weikun , Cao Jian , Xie Qiang , Deng Chengcheng , Huang Xinmei , Hu Shiping
Published: September 2023

Purpose

To investigate Src-like adaptor 2 gene (SLA2) expression in head and neck squamous cell carcinoma (HNSCC), its potential prognostic value, and its effect on immune cell infiltration.

Methods

Through a variety of bioinformatics analyses, we extracted and analyzed data sets from the Cancer Genome Atlas (TCGA), Tumor Immune Estimation Resource (TIMER), and Gene Expression Profile Interaction Analysis (GEPIA) to analyze the correlation between SLA2 and the prognosis, immune checkpoint, tumor microenvironment (TME) and immune cell infiltration of HNSCC, and to explore its potential oncogenic mechanism. To further explore the potential role of SLA2 in HNSCC by Gene ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.

Results

SLA2 messenger ribonucleic acid (mRNA) levels were increased in HNSCC tumor tissues compared with normal tissues. In addition, we found that SLA2 may be an independent prognostic factor for HNSCC, and high SLA2 expression is associated with favorable prognosis in HNSCC. SLA2 expression was positively correlated with B cells, cluster of differentiation 8-positive T cells (CD8 + T cells), cluster of differentiation 4-positive T cells (CD4 + T cells), macrophages, neutrophil and dendritic cells infiltration. SLA2 has also been shown to co-express immune-related genes and immune checkpoints. Significant GO term analysis by Gene Set Enrichment Analysis (GSEA) indicated that genes correlated with SLA2 were located mainly in the side of membrane, receptor complex, secretory granule membrane, endocytic vesicle, membrane region, and endosome membrane, where they were involved in leukocyte cell–cell adhesion, response to interferon-gamma, and regulation of immune effector process. These related genes also served as antigen binding, cytokine receptor activity, phosphatidylinositol 3-kinase activity, peptide receptor activity, Src homology domain 3 (SH3) domain binding, and cytokine receptor binding. KEGG pathway analysis demonstrated that these genes related to SLA2 were mainly enriched in signal pathways, such as hematopoietic cell lineage, cell adhesion molecules (CAMs), natural killer cell mediated cytotoxicity, measles, and chemokine signaling pathway.

Conclusions

SLA2 is increased in HNSCC, and high SLA2 expression is associated with favorable prognosis. SLA2 may affect tumor development by regulating tumor infiltrating cells in TME. SLA2 may be a potential target for immunotherapy.
Biphenyls and dibenzofurans of the rosaceous subtribe Malinae and their role as phytoalexins
Authors: Busnena Belnaser A. , Beerhues Ludger , Liu Benye
Published: September 2023

Main conclusion

Biphenyl and dibenzofuran phytoalexins are differentially distributed among species of the rosaceous subtribe Malinae, which includes apple and pear, and exhibit varying inhibitory activity against phytopathogenic microorganisms.

Biphenyls and dibenzofurans are specialized metabolites, which are formed in species of the rosaceous subtribe Malinae upon elicitation by biotic and abiotic inducers. The subtribe Malinae (previously Pyrinae) comprises approximately 1000 species, which include economically important fruit trees such as apple and pear. The present review summarizes the current status of knowledge of biphenyls and dibenzofurans in the Malinae, mainly focusing on their role as phytoalexins. To date, 46 biphenyls and 41 dibenzofurans have been detected in 44 Malinae species. Structurally, 54 simple molecules, 23 glycosidic compounds and 10 miscellaneous structures were identified. Functionally, 21 biphenyls and 21 dibenzofurans were demonstrated to be phytoalexins. Furthermore, their distribution in species of the Malinae, inhibitory activities against phytopathogens, and structure–activity relationships were studied. The most widely distributed phytoalexins of the Malinae are the three biphenyls aucuparin (3), 2ʹ-methoxyaucuparin (7), and 4ʹ-methoxyaucuparin (9) and the three dibenzofurans α-cotonefuran (47), γ-cotonefuran (49), and eriobofuran (53). The formation of biphenyl and dibenzofuran phytoalexins appears to be an essential defense weapon of the Malinae against various stresses. Manipulating phytoalexin formation may enhance the disease resistance in economically important fruit trees. However, this approach requires an extensive understanding of how the compounds are formed. Although the biosynthesis of biphenyls was partially elucidated, formation of dibenzofurans remains largely unclear. Thus, further efforts have to be made to gain deeper insight into the distribution, function, and metabolism of biphenyls and dibenzofurans in the Malinae.
Ecosystem engineering and leaf quality together affect arthropod community structure and diversity on white oak (Quercus alba L.)
Authors: Reinhardt Jason R. , Marquis Robert J.
Published: September 2023

Shelter building caterpillars act as ecosystem engineers by creating and maintaining leaf shelters, which are then colonized by other arthropods. Foliage quality has been shown to influence initial colonization by shelter-building caterpillars. However, the effects of plant quality on the interactions between ecosystem engineers and their communities have yet to be studied at the whole plant level. We examined how leaf tying caterpillars, as ecosystem engineers, impact arthropod communities on Quercus alba (white oak), and the modifying effect of foliage quality on these interactions. We removed all leaf tying caterpillars and leaf ties on 35 Q. alba saplings during the season when leaf tying caterpillars were active (June–September), and compared these leaf tie removal trees to 35 control trees whose leaf ties were left intact. Removal of these ecosystem engineers had no impact on overall arthropod species richness, but reduced species diversity, and overall arthropod abundance and that of most guilds, and changed the structure of the arthropod community as the season progressed. There was an increase in plant-level species richness with increasing number of leaf ties, consistent with Habitat Diversity Hypothesis. In turn, total arthropod density, and that of both leaf tying caterpillars and free-feeding caterpillars were affected by foliar tannin and nitrogen concentrations, and leaf water content. The engineering effect was greatest on low quality plants, consistent with the Stress-Gradient Hypothesis. Our results demonstrate that interactions between ecosystem engineering and plant quality together determine community structure of arthropods on Q. alba in Missouri.
Implementation of the Enhanced Recovery After Surgery (ERAS®) program in neurosurgery
Authors: Belouaer Amani , Cossu Giulia , Papadakis Georgios E. , Gaudet John G. , Perez Maria-Helena , Chanez Vivianne , Boegli Yann , Mury Caroline , Peters David , Addor Valérie , Levivier Marc , Daniel Roy Thomas , Demartines Nicolas , Messerer Mahmoud
Published: September 2023

Background

Over the past decade, Enhanced Recovery After Surgery (ERAS®) guidelines have been proven to simplify postoperative care and improve recovery in several surgical disciplines. The authors set out to create and launch an ERAS® program for cranial neurosurgery that meets official ERAS® Society standards. The authors summarize the successive steps taken to achieve this goal in two specific neurosurgical conditions and describe the challenges they faced.

Methods

Pituitary neuroendocrine tumors (Pit-NET) resected by a transsphenoidal approach and craniosynostosis (Cs) repair were selected as appropriate targets for the implementation of ERAS® program in the Department of Neurosurgery. A multidisciplinary team with experience in managing these pathologies was created. A specialized ERAS® nurse coordinator was hired. An ERAS® certification process was performed involving 4 seminars separated by 3 active phases under the supervision of an ERAS® coach.

Results

The ERAS® Pit-NET team included 8 active members. The ERAS® Cs team included 12 active members. Through the ERAS® certification process, areas for improvement were identified, local protocols were written, and the ERAS® program was implemented. Patient-centered strategies were developed to increase compliance with the ERAS® protocols. A prospective database was designed for ongoing program evaluation. Certification was achieved in 18 months. Direct costs and time requirements are reported.

Conclusion

Successful ERAS® certification requires a committed multidisciplinary team, an ERAS® coach, and a dedicated nurse coordinator.
Solvothermal synthesis of cobalt PCP pincer complexes from [Co2(CO)8]
Authors: Schratzberger Heiko , Himmelbauer Daniel , Eder Wolfgang , Weiser Michael , Stöger Berthold , Kirchner Karl
Published: September 2023

Treatment of [Co2(CO)8] with the ipso-substituted P(C-X)PY ligands (X = Br, Cl; R = iPr, tBu) bearing Y = NH and CH2 linkers under solvothermal conditions affords the five-coordinate Co(I) and Co(III) complexes [CoI(PCPY-R)(CO)2] and [CoIII(PCPY-R)X2]. The later are paramagnetic exhibiting a solution magnetic moment in the range of 3.0–3.3 μB which is consistent with a d6 intermediate spin system corresponding to two unpaired electrons. In the case of P(C-X)PY ligands (X = Br, Cl; R = tBu; Y = NH) the formation of the square planar Co(II) complex [Co(PCPNH-tBu)X] was favored. This complex gives rise to a magnetic moment of 1.8 μB being consistent with a d7 low spin system corresponding to one unpaired electron. All complexes are characterized by means of spectroscopic techniques (NMR, IR), HR-MS. Representative complexes were also characterized by X-ray crystallography.

Graphical abstract
River benthic macroinvertebrates and environmental DNA metabarcoding: a scoping review of eDNA sampling, extraction, amplification and sequencing methods
Authors: Vourka Aikaterini , Karaouzas Ioannis , Parmakelis Aristeidis
Published: September 2023

There is a growing body of literature on the use of molecular methods for the ecological assessment of rivers based on benthic macroinvertebrates. Previous research has established the benefits of the use of environmental DNA (eDNA) to assess benthic macroinvertebrate communities as being more efficient, less subjective, and non-invasive compared to traditional methods. The aim of this review is to synthesize the existing knowledge on eDNA sampling, extraction, amplification and sequencing methods regarding river benthic macroinvertebrate metabarcoding studies. Literature searches were performed using two online databases, and following a screening process, 46 papers published between 2012 and 2022 met the eligibility criteria to be included in the review. Since the use of river macrobial eDNA in ecology is a fast-evolving field, the results showed that the methodologies used vary considerably among studies. A variety of filters are used for capturing eDNA from water or preservative ethanol and different sources of eDNA (i.e., sediment, biofilm) are also explored. This review identified 12 different extraction methods and 15 different primer pairs that were used more than once in benthic macroinvertebrate eDNA metabarcoding studies. Therefore, there is a need for standardization of some key steps of the eDNA metabarcoding process to increase the comparability of the results and the robustness of the methods for further implementation into large-scale monitoring programs.
Resource recovery and reduction of tidal waste to produce cellulose through sulphur-free pulping
Authors: Moral Ana , Greyer Valeria , Aguado Roberto J. , Tijero Antonio
Published: September 2023

The transition towards a sustainable cellulose industry needs more environmentally friendly technologies, including the use of lignocellulosic waste as alternative raw materials. This work shows the potential of dead biomass from the Andalusian Mediterranean coastline to obtain cellulosic pulp. With this purpose, predominant species in onshore residues (mainly Posidonia oceanica) were prepared, characterized, and subjected to a soda-anthraquinone pulping process. A central composite design was defined in order to relate the process conditions to the composition and characteristics of the cellulose pulp. It was concluded that polynomial models encompassing linear, quadratic, and binary effect parameters reproduce the experimental results satisfactorily. Results allowed to choose high anthraquinone dosage, alkali charge and temperature, low liquid/solid ratio and short time as a compromise solution, by which the brightness, ethanol–benzene extractives content, kappa number, lignin content and viscosity were near their optimal. Low values of liquid/solid ratio reduce water consumption, which is of utmost importance in cellulose industries likewise short time treatments to save energy.
“Missing the Community for the Dots”: Newspaper Crime Maps, Territorial Stigma and Visual Criminology
Authors: Schwarze Tilman
Published: September 2023

By drawing on research on territorial stigmatization, critical cartography, and visual criminology, this article develops a critical literacy of crime maps to scrutinize their ability to function as adequate representations of crime. Focusing on online newspaper crime maps of homicides published by one of Chicago’s major newspapers, the Chicago Sun-Times, this article argues that crime maps assign a criminogenic character to urban spaces by spatially inscribing homicides onto community maps. In particular, this article devotes attention to the power of crime visualizations through cartography, developing strategies to educate readers about how to critically think about and read crime maps’ visualizations of the spatiality of crime. This article is intended as both a critique of crime maps’ spatio-visual practices as well as an attempt to develop a critical literacy of crime maps.
Who Identifies with the Aggregates? Philosophical Implications of the Selected Khandha Passages in the Nikāyas
Authors: Polak Grzegorz
Published: September 2023

In this paper, I discuss some philosophical problems connected with the notion of regarding the aggregates (khandha) as self in the Nikāyas. In particular, I focus on the attitude represented by the formula “I am this” (esohamasmi) which may be labeled as that of identifying with the aggregates. In the first part of the paper, I point out and analyze certain similes contained in the Nikāyas which may be read as implying the existence of a distinction between the aggregates and the individual who regards them as self. Then, I consider a hypothesis that the aggregates are not objective constituents of a human being but subjectively experienced representational phenomena. I argue that several Nikāya texts imply the existence of important aspects of a human being, in particular cognitive ones, which are not conceptualized in the terms of the five aggregates. I also discuss the possibility of interpreting khandhas as not active in character but as resultant of other cognitive processes. In the last part of the paper I offer a hypothesis that one of the key aspects of regarding the aggregates as self lies in the identification of a human individual with one’s own phenomenal self-representation which results in a mistaken projection of the notions of agency, identity and subjectivity onto something that is inherently devoid of it. I also consider certain parallels between the ideas present in the Nikāyas and the concepts of the modern philosophy of mind.
More insect species are supported by green roofs near public gardens
Authors: Hussain Raja Imran , Frank Thomas , Kratschmer Sophie
Published: September 2023

Rapid urbanization is among the factors that decrease insect diversity. However, by offering suitable habitats, green roofs could lessen this adverse effect. Certain factors, like a nearby public garden, could be useful predictor variables to analyze to what extent green roofs can support insect communities. The study aimed to measure the insect diversity on intensive green roofs located near public gardens and on more isolated green roofs, within an urban setting. Insect species richness, abundance, and assemblages on the green roofs near public gardens differed from those on isolated green roofs. Results indicate that green roofs near public gardens will host more species, especially pollinators, consequently lessening urbanization’s negative effect. To properly understand how landscape factors impact insect communities on green roofs, future studies on green roofs’ biodiversity should consider public gardens and their influence on urban biodiversity.

Implications for insect conservation

The planning and establishment of a green roof near public gardens would probably enhance biodiversity conservation in an urban setting.
Improving information retrieval through correspondence analysis instead of latent semantic analysis
Authors: Qi Qianqian , Hessen David J. , van der Heijden Peter G. M.
Published: September 2023

The initial dimensions extracted by latent semantic analysis (LSA) of a document-term matrix have been shown to mainly display marginal effects, which are irrelevant for information retrieval. To improve the performance of LSA, usually the elements of the raw document-term matrix are weighted and the weighting exponent of singular values can be adjusted. An alternative information retrieval technique that ignores the marginal effects is correspondence analysis (CA). In this paper, the information retrieval performance of LSA and CA is empirically compared. Moreover, it is explored whether the two weightings also improve the performance of CA. The results for four empirical datasets show that CA always performs better than LSA. Weighting the elements of the raw data matrix can improve CA; however, it is data dependent and the improvement is small. Adjusting the singular value weighting exponent often improves the performance of CA; however, the extent of the improvement depends on the dataset and the number of dimensions.
Evolutionary algorithms for a simheuristic optimization of the product-service system design
Authors: Meeß Henri , Herzog Michael , Alp Enes , Kuhlenkötter Bernd
Published: September 2023

Offering Product-Service Systems (PSS) becomes an established strategy for companies to increase the provided customer value and ensure their competitiveness. Designing PSS business models, however, remains a major challenge. One reason for this is the fact that PSS business models are characterized by a long-term nature. Decisions made in the development phase must take into account possible scenarios in the operational phase. Risks must already be anticipated in this phase and mitigated with appropriate measures. Another reason for the design phase being a major challenge is the size of the solution space for a possible business model. Developers are faced with a multitude of possible business models and have the challenge of selecting the best one. In this article, a simheuristic optimization approach is developed to test and evaluate PSS business models in the design phase in order to select the best business model configuration beforehand. For optimization, a proprietary evolutionary algorithm is developed and tested. The results validate the suitability of the approach for the design phase and the quality of the algorithm for achieving good results. This could even be transferred to already established PSS.
Getting machines to do your dirty work
Authors: Francis Tomi , Karhu Todd
Published: September 2023

Autonomous systems are machines that can alter their behavior without direct human oversight or control. How ought we to program them to behave? A plausible starting point is given by the Reduction to Acts Thesis, according to which we ought to program autonomous systems to do whatever a human agent ought to do in the same circumstances. Although the Reduction to Acts Thesis is initially appealing, we argue that it is false: it is sometimes permissible to program a machine to do something that it would be wrong for a human to do. We advance two main arguments for this claim. First, the way an autonomous system will behave can be known in advance. This knowledge can indirectly affect the behavior of other agents, while the same may not be true at the time the system actually executes its programming. Second, a lack of knowledge of the identities of the victims and beneficiaries can provide a justification during the programming phase that would be unavailable to an agent at the time the autonomous system executes its programming.
Towards a better understanding of soil nutrient dynamics and P and K uptake
Authors: Schut Antonius G. T. , Reymann W.
Published: September 2023

Aim

Balanced crop nutrition is key to improve nutrient use efficiency and reduce environmental impact of farming systems. We developed and tested a dynamic model to predict the uptake of P and K in long-term experiments to better understand how changes in soil nutrient pools affect nutrient availability in crop rotations.

Methods

Our RC-KP model includes labile and stable pools for P and K, with separate labile pools for placed P and organic fertilizers including farm yard manure (FYM). Pool sizes and crop-specific relative uptake rates determined potential uptake. Actual crop uptake from labile pools was based on concepts developed by Janssen et al. (Geoderma 46:299-318, 1990). The model was calibrated on three long-term experiments from Kenia (Siaya), Germany (Hanninghof) and the United Kingdom (Broadbalk) to estimate parameter values for crop-specific relative uptake rates and site-specific relative transfer rates.

Results

The model described N, P and K uptake accurately with a Nash-Sutcliff modelling efficiency of 0.6–0.9 and root mean squared errors of 2.6–3.4 kg P ha−1 and 14–20 kg K ha−1. Excluding organic labile pools did not affect model accuracy in Broadbalk in contrast to Hanninghof where Mg deficiencies affected crop uptakes in treatments without Mg or FYM.

Conclusions

This relatively simple model provides a novel approach to accurately estimate N, P and K uptake and explore short- and long-term effects of fertilizers in crop rotations. Interactions between limiting nutrients affecting actual nutrient uptake were captured well, providing new options to include N, P and K limitations in crop growth models.
Where Merleau-Ponty Meets Dewey: Habit, Embodiment, and Education
Authors: Thorburn Malcolm , Stolz Steven A.
Published: September 2023

This paper utilises selective writings by John Dewey and Maurice Merleau-Ponty as the conceptual basis for considering how an enhanced synergistic focus on habit and embodiment could support practice gains in schools. The paper focuses on Dewey’s belief that established habits can help students to incorporate experiences into evaluations of educational progress and Merleau-Ponty’s spotlight on the body-subject, and how it provides a holistic way of conceiving relations that avoid over privileging abstraction and cognition and under-representing the centrality of the body in human experience. We then evaluate the role of language and reflection in relation to habit, embodiment, and education before discussing some of the general educational practice considerations which might improve the quality of students learning experiences. In conclusion, we consider the possibilities for Dewey’s pragmatism to function as a theoretical bridge to Merleau-Ponty’s phenomenology of perception and the nature of experience. We argue that while it is important to recognise points of difference, in relation to philosophical treatments of habit and embodiment, it is also important to consider the overlap and new connections between Merleau-Ponty and Dewey which are valuable to take forward in contemporary education.
Perspectives on prostate cancer: advances and pending challenges for a multidisciplinary oncological approach in South America
Authors: Diaz Kevin A. , Amaya Sandra Liliana , García-Perdomo Herney Andrés
Published: September 2023

Prostate cancer is one of the tumors with the highest incidence and mortality among men worldwide, and this situation is no different in South America. However, epidemiological data are highly variable for each country and even more so than in North America. These data may be influenced by the very low rate of early detection of disease, availability of diagnostic methods, proper data collection, and limited access to specialized multidisciplinary treatment. For many South American countries, academic referral centers can only offer state-of-the-art diagnostics and multidisciplinary cancer treatment for patients who live in or can travel to large cities, so most patients are cared for by non-expert urologists with limited resources, which can have a negative impact on their prognosis and worsen oncologic outcomes. We aimed to show the clinical management of prostate cancer patients, the current advances in management, limitations present in South America, and how a multidisciplinary approach in referral cancer centers conformed of specialized urologists, medical oncologists, and mental health professionals can maximize patient outcomes.
Structural similarity of human papillomavirus E4 and polyomaviral VP4 exhibited by genomic analysis of the common kestrel (Falco tinnunculus) polyomavirus
Authors: Fehér Enikő , Kaszab Eszter , Mótyán János András , Máté Dóra , Bali Krisztina , Hoitsy Márton , Sós Endre , Jakab Ferenc , Bányai Krisztián
Published: September 2023

Polyomaviruses are widely distributed viruses of birds that may induce developmental deformities and internal organ disorders primarily in nestlings. In this study, polyomavirus sequence was detected in kidney and liver samples of a common kestrel (Falco tinnunculus) that succumbed at a rescue station in Hungary. The amplified 5025 nucleotide (nt) long genome contained the early (large and small T antigen, LTA and STA) and late (viral proteins, VP1, VP2, VP3) open reading frames (ORFs) typical for polyomaviruses. One of the additional putative ORFs (named VP4) showed identical localization with the VP4 and ORF-X of gammapolyomaviruses, but putative splicing sites could not be found in its sequence. Interestingly, the predicted 123 amino acid (aa) long protein sequence showed the highest similarity with human papillomavirus E4 early proteins in respect of the aa distribution and motif arrangement implying similar functions. The LTA of the kestrel polyomavirus shared <59.2% nt and aa pairwise identity with the LTA sequence of other polyomaviruses and formed a separated branch in the phylogenetic tree among gammapolyomaviruses. Accordingly, the kestrel polyomavirus may be the first member of a novel species within the Gammapolyomavirus genus, tentatively named Gammapolyomavirus faltin.
Would you let this guy into a bar? Identifying cues that signal a perceived increase in the propensity for violence of potential bar patrons
Authors: Summerell Elizabeth , Fanous Geneviv , Denson Thomas F.
Published: September 2023

Objectives

Bars, pubs, and clubs are hotspots for alcohol-related aggression. Consequently, admittance decisions made by security personnel have important implications for the safety inside these venues. However, the cues used by security personnel to determine the potential for intoxicated violence and inform admittance decisions vary substantially.

Methods

Here, we manipulate theoretically and practically relevant cues to determine the effects of their utilization on perceptions of violence and admittance decisions. Participants viewed images of real inmates convicted of violent or non-violent crimes, accompanied by cues derived from interviews with security staff.

Results

We found that body tenseness, drug and alcohol intoxication, searching behavior, avoidant behavior, and neck and face tattoos were positively associated with a greater perceived likelihood of violence. Admittance decisions mirrored these findings. Students and security personnel differed in their utilization of some of the cues.

Conclusions

To our knowledge, the current research was the first to take a quantitative approach to understanding the cues that security personnel and young adults might use to identify potentially violent patrons. These finding may help inform training for security personnel.
Range shift and introgression of three Japanese Abies species: insights from population genetic data and species distribution modeling
Authors: Uchiyama Kentaro , Fujii Sayaka , Tsuyama Ikutaro , Tanaka Nobuyuki , Setsuko Suzuki , Kimura Megumi K. , Suyama Yoshihisa , Tsumura Yoshihiko
Published: September 2023

Range shifts during the Pleistocene shaped the unique phylogeographical structures of many species. Pleistocene range shifts gave currently allopatric species opportunities to occur in sympatry, likely resulting in ancient introgressions between related taxa. In our study, we investigate the range shifts and introgression patterns of three Japanese Abies species (A. firma, A. homolepis, and A. veitchii) by employing an extensive survey of 43 populations. This survey includes comprehensive analysis of both mitochondrial (mtDNA) and nuclear (18 microsatellites) genomes, in combination with species distribution modeling (SDM). It is important to note that these two types of markers provide distinct and complementary information, as they have different modes of inheritance and mutation rates. Bayesian clustering analysis indicates that the three species were clearly separated, with the exception of the A. homolepis var. umbellata population, which is considered a natural hybrid between A. homolepis and A. firma. However, mtDNA haplotypes of the four northern populations of A. firma were entirely replaced by two major haplotypes of A. homolepis and A. veitchii. The results of neighbor-net, NewHybrids, STRUCTURE analyses, and SDM suggest that historical introgression between species occurred in each geographic region, with mtDNA capture being the likely mechanism. However, contrary to these findings, the ABC coalescent analysis did not support an ancient introgression. Therefore, further validation with genome-wide level data is needed to clarify this issue. Our conclusion is that climate-induced range shifts during the Pleistocene/Holocene likely played a crucial role in the observed patterns of introgression in these species.