bluuebunny/crossref_metadata_2025 huggingface dataset:
Forecasting emotions: exploring the relationship between self-control, affective forecasting, and self-regulatory behavior
Authors: Strömbäck Camilla
Published: June 2024

Empirical evidence has shown that people with better self-control to a greater extent have the self-regulatory ability to act in line with their long-term goals. In this pre-registered study, the relationship between self-control and self-regulatory behavior was investigated both directly and indirectly, i.e., through affective forecasting ability. This is of great interest as it is necessary to be able to forecast one's emotional response to future events in order to make choices that maximize one's happiness. However, in a laboratory experiment with undergraduate students, I found no evidence of self-control being associated with affective forecasting ability, or that people with better self-control more often acted in a way that maximized their expected happiness.
Zufallsbefunde: submuköser Tumor bei der Gastroskopie
Authors: Tribl Barbara
Published: June 2024

Submuköse Tumoren sind meist asymptomatisch und sind in der Regel ein Zufallsbefund bei der Gastroskopie. Submuköse Tumoren können aber auch symptomatisch werden im Rahmen einer gastrointestinalen Blutung bzw. durch eine Eisenmangelanämie oder aber durch Schmerzen infolge einer Obstruktion – bevorzugt an anatomischen Engstellen wie an der Cardia ventriculi oder dem Pylorus. Nur ein Teil der submukösen Tumoren benötigt eine Resektion bzw. eine Surveillance. Die Endosonographie mit der Option der Gewebegewinnung ist ein wesentlicher Bestandteil der Diagnostik und soll häufiger zur Diagnosesicherung eingesetzt werden. Es besteht die Empfehlung, nur Läsionen zu resezieren, die ein malignes Risiko haben oder Symptome verursachen.
Research advances on standards and processing methods of texture-modified foods for dysphagia: a review
Authors: Liu Tong , Liu Caiyun , Wang Xin
Published: June 2024

As a remarkably common clinical symptom of many diseases, dysphagia has become a major public health issue. Texture-modified foods is a widespread therapeutic strategy for dysphagia, but the design of texture-modified foods is a challenging task. Considering that the implementation of standardized terms and definitions are helpful for the texture modification of food, literature reviews has been conducted in this paper and relevant classification standards in different countries were summarized. And the techniques and means for food texture modification, such as traditional dietary softening approaches, use of biopolymers, as well as emerging structural technologies were also discussed. In addition, potential research directions have been suggested for the design of texture-modified foods.
Thermal performance of a novel Trombe wall enhanced by a solar energy focusing approach
Authors: Dong Xianzhang , Xiao Huigang , Ma Minglei
Published: June 2024

The Trombe wall is a passive solar building exterior wall system proposed by Professor Felix Trombe in France, which can collect solar energy to heat buildings without additional energy consumption, making it a focal point of research in building energy conservation. However, its effectiveness is constrained by the low density of solar radiation in winter and the potential for overheating in summer. This study introduces a novel Trombe wall designed to address these issues through a focused strategy, enabling automatic transition between heating during winter and shading during summer. The thermal performance parameters of the novel Trombe walls in both winter and summer seasons are examined, and their energy consumption is assessed using experimental research methodologies. Findings indicate that the novel Trombe wall facilitates greater energy savings in both winter and summer. When compared with traditional Trombe walls, the novel Trombe wall achieves a significant reduction in energy consumption, with up to 55 W/m2 in heating load during winter and 47 W/m2 in cooling load during summer. The introduction of this new system holds substantial potential for the realization of zero-energy buildings.
Deep reinforcement learning for inverting earthquake focal mechanism and its potential application to marine earthquakes
Authors: Kuang Wenhuan , Zou Zhihui , Xing Junhui , Wei Wei
Published: June 2024

Earthquake data are one of the key means by which to explore our planet. At a large scale, the layered structure of the Earth is revealed by the seismic waves of natural earthquakes that go deep into its inner core. At a local scale, seismology for exploration has successfully been employed to discover massive fossil energies. As the volume of recorded seismic data becomes greater, intelligent methods for processing such a volume of data are eagerly anticipated. In particular, earthquake focal mechanisms are important for assessing the severity of tsunamis, characterizing seismogenic faults, and investigating the stress perturbations that follow a major earthquake. Here, we report a novel deep reinforcement learning method for inverting the earthquake focal mechanism. Unlike more typical deep learning applications, which require a large training dataset, a deep reinforcement learning system learns by itself. We demonstrate the validity and efficacy of the proposed deep reinforcement learning method by applying it to the Mw 7.1 mainshock of the Ridgecrest earthquakes in southern California. In the foreseeable future, deep learning technologies may greatly contribute to our understanding of the oceanographic process. The proposed method may help us understand the mechanism of marine earthquakes.
Mitigating Toxic Metal Exposure Through Leafy Greens: A Comprehensive Review Contrasting Cadmium and Lead in Spinach
Authors: Seyfferth Angelia L. , Limmer Matt A. , Runkle Benjamin R. K. , Chaney Rufus L.
Published: June 2024

Metals and metalloids (hereafter, metal(loid)s) in plant‐based foods are a source of exposure to humans, but not all metal(loid)‐food interactions are the same. Differences exist between metal(loid)s in terms of their behavior in soils and in how they are taken up by plants and stored in the edible plant tissue/food. Thus, there cannot be one consistent solution to reducing toxic metal(loid)s exposure to humans from foods. In addition, how metal(loid)s are absorbed, distributed, metabolized, and excreted by the human body differs based on both the metal(loid), other elements and nutrients in the food, and the nutritional status of the human. Initiatives like the United States Food and Drug Administration's Closer to Zero initiative to reduce the exposure of young children to the toxic elements cadmium, lead, arsenic, and mercury from foods warrant careful consideration of each metal(loid) and plant interaction. This review explores such plant‐metal(loid) interactions using the example of spinach and the metals cadmium and lead. This review highlights differences in the magnitude of exposure, bioavailability, and the practicality of mitigation strategies while outlining research gaps and future needs. A focus on feasibility and producer needs, informed via stakeholder interviews, emphasizes the need for better analytical testing facilities and grower and consumer education. More research should focus on minimization of chloride inputs for leafy greens to lessen plant‐availability of Cd and the role of oxalate in reducing Cd bioavailability from spinach. These findings are applicable to other leafy greens (e.g., kale, lettuce), but not for other plants or metal(loid)s.
Whistler Waves in the Quasi‐Parallel and Quasi‐Perpendicular Magnetosheath
Authors: Svenningsson I. , Yordanova E. , Khotyaintsev Yu. V. , André M. , Cozzani G. , Steinvall K.
Published: June 2024

In the Earth's magnetosheath (MSH), several processes contribute to energy dissipation and plasma heating, one of which is wave‐particle interactions between whistler waves and electrons. However, the overall impact of whistlers on electron dynamics in the MSH remains to be quantified. We analyze 18 hr of burst‐mode measurements from the Magnetospheric Multiscale (MMS) mission, including data from the unbiased magnetosheath campaign during February‐March 2023. We present a statistical study of 34,409 whistler waves found using automatic detection. We compare wave occurrence in the different MSH geometries and find three times higher occurrence in the quasi‐perpendicular MSH compared to the quasi‐parallel case. We also study the wave properties and find that the waves propagate quasi‐parallel to the background magnetic field, have a median frequency of 0.2 times the electron cyclotron frequency, median amplitude of 0.03–0.06 nT (30–60 pT), and median duration of a few tens of wave periods. The whistler waves are preferentially observed in local magnetic dips and density peaks and are not associated with an increased temperature anisotropy. Also, almost no whistlers are observed in regions with parallel electron plasma beta lower than 0.1. Importantly, when estimating pitch‐angle diffusion times we find that the whistler waves cause significant pitch‐angle scattering of electrons in the MSH.
C-terminally phosphorylated p27 activates self-renewal driver genes to program cancer stem cell expansion, mammary hyperplasia and cancer
Authors: Razavipour Seyedeh Fatemeh , Yoon Hyunho , Jang Kibeom , Kim Minsoon , Nawara Hend M. , Bagheri Amir , Huang Wei-Chi , Shin Miyoung , Zhao Dekuang , Zhou Zhiqun , Van Boven Derek , Briegel Karoline , Morey Lluis , Ince Tan A. , Johnson Michael , Slingerland Joyce M.
Published: June 2024

In many cancers, a stem-like cell subpopulation mediates tumor initiation, dissemination and drug resistance. Here, we report that cancer stem cell (CSC) abundance is transcriptionally regulated by C-terminally phosphorylated p27 (p27pT157pT198). Mechanistically, this arises through p27 co-recruitment with STAT3/CBP to gene regulators of CSC self-renewal including MYC, the Notch ligand JAG1, and ANGPTL4. p27pTpT/STAT3 also recruits a SIN3A/HDAC1 complex to co-repress the Pyk2 inhibitor, PTPN12. Pyk2, in turn, activates STAT3, creating a feed-forward loop increasing stem-like properties in vitro and tumor-initiating stem cells in vivo. The p27-activated gene profile is over-represented in STAT3 activated human breast cancers. Furthermore, mammary transgenic expression of phosphomimetic, cyclin-CDK-binding defective p27 (p27CK-DD) increases mammary duct branching morphogenesis, yielding hyperplasia and microinvasive cancers that can metastasize to liver, further supporting a role for p27pTpT in CSC expansion. Thus, p27pTpT interacts with STAT3, driving transcriptional programs governing stem cell expansion or maintenance in normal and cancer tissues.
An electrochemically responsive B–O dynamic bond to switch photoluminescence of boron-nitrogen-doped polyaromatics
Authors: Yang Baige , Zhang Yu-Mo , Wang Chunyu , Gu Chang , Li Chenglong , Yin Hang , Yan Yan , Yang Guojian , Zhang Sean Xiao-An
Published: June 2024

Boron-doped polycyclic aromatic hydrocarbons exhibit excellent optical properties, and regulating their photophysical processes is a powerful strategy to understand the luminescence mechanism and develop new materials and applications. Herein, an electrochemically responsive B–O dynamic coordination bond is proposed, and used to regulate the photophysical processes of boron-nitrogen-doped polyaromatic hydrocarbons. The formation of the B–O coordination bond under a suitable voltage is confirmed by experiments and theoretical calculations, and B–O coordination bond can be broken back to the initial state under opposite voltage. The whole process is accompanied by reversible changes in photophysical properties. Further, electrofluorochromic devices are successfully prepared based on the above electrochemically responsive coordination bond. The success and harvest of this exploration are beneficial to understand the luminescence mechanism of boron-nitrogen-doped polyaromatic hydrocarbons, and provide ideas for design of dynamic covalent bonds and broaden material types and applications.
Hyperbaric oxygen enhances tumor penetration and accumulation of engineered bacteria for synergistic photothermal immunotherapy
Authors: Xu Ke-Fei , Wu Shun-Yu , Wang Zihao , Guo Yuxin , Zhu Ya-Xuan , Li Chengcheng , Shan Bai-Hui , Zhang Xinping , Liu Xiaoyang , Wu Fu-Gen
Published: June 2024

Bacteria-mediated cancer therapeutic strategies have attracted increasing interest due to their intrinsic tumor tropism. However, bacteria-based drugs face several challenges including the large size of bacteria and dense extracellular matrix, limiting their intratumoral delivery efficiency. In this study, we find that hyperbaric oxygen (HBO), a noninvasive therapeutic method, can effectively deplete the dense extracellular matrix and thus enhance the bacterial accumulation within tumors. Inspired by this finding, we modify Escherichia coli Nissle 1917 (EcN) with cypate molecules to yield EcN-cypate for photothermal therapy, which can subsequently induce immunogenic cell death (ICD). Importantly, HBO treatment significantly increases the intratumoral accumulation of EcN-cypate and facilitates the intratumoral infiltration of immune cells to realize desirable tumor eradication through photothermal therapy and ICD-induced immunotherapy. Our work provides a facile and noninvasive strategy to enhance the intratumoral delivery efficiency of natural/engineered bacteria, and may promote the clinical translation of bacteria-mediated synergistic cancer therapy.
Accelerometer-derived physical activity and mortality in individuals with type 2 diabetes
Authors: Cao Zhi , Min Jiahao , Chen Han , Hou Yabing , Yang Hongxi , Si Keyi , Xu Chenjie
Published: June 2024

Physical activity (PA) has been shown to reduce diabetes mortality, but largely based on imprecise self-reported data, which may hinder the development of related recommendations. Here, we perform a prospective cohort study of 4003 individuals with type 2 diabetes (T2D) from the UK Biobank with a median follow-up of 6.9 years. Duration and intensity of PA are measured by wrist-worn accelerometers over a 7-day period. We observe L-shaped associations of longer duration of PA, regardless of PA intensity, with risks of all-cause and cancer mortality, as well as a negatively linear association with cardiovascular disease mortality. 18.8%, 28.0%, and 31.1% of deaths are attributable to the lowest level of light-intensity PA, moderate-intensity PA, and vigorous-intensity PA, respectively. Collectively, our findings provide insights for clinical guidelines that should highlight the potential value of adherence to greater intensity and duration of PA for patients with T2D.
Low potassium activation of proximal mTOR/AKT signaling is mediated by Kir4.2
Authors: Zhang Yahua , Bock Fabian , Ferdaus Mohammed , Arroyo Juan Pablo , L Rose Kristie , Patel Purvi , Denton Jerod S. , Delpire Eric , Weinstein Alan M. , Zhang Ming-Zhi , Harris Raymond C. , Terker Andrew S.
Published: June 2024

The renal epithelium is sensitive to changes in blood potassium (K+). We identify the basolateral K+ channel, Kir4.2, as a mediator of the proximal tubule response to K+ deficiency. Mice lacking Kir4.2 have a compensated baseline phenotype whereby they increase their distal transport burden to maintain homeostasis. Upon dietary K+ depletion, knockout animals decompensate as evidenced by increased urinary K+ excretion and development of a proximal renal tubular acidosis. Potassium wasting is not proximal in origin but is caused by higher ENaC activity and depends upon increased distal sodium delivery. Three-dimensional imaging reveals Kir4.2 knockouts fail to undergo proximal tubule expansion, while the distal convoluted tubule response is exaggerated. AKT signaling mediates the dietary K+ response, which is blunted in Kir4.2 knockouts. Lastly, we demonstrate in isolated tubules that AKT phosphorylation in response to low K+ depends upon mTORC2 activation by secondary changes in Cl- transport. Data support a proximal role for cell Cl- which, as it does along the distal nephron, responds to K+ changes to activate kinase signaling.
Neurovascular and immune factors of vulnerability of substantia nigra dopaminergic neurons in non-human primates
Authors: Balzano Tiziano , del Rey Natalia López-González , Esteban-García Noelia , Reinares-Sebastián Alejandro , Pineda-Pardo José A. , Trigo-Damas Inés , Obeso José A. , Blesa Javier
Published: June 2024

Dopaminergic neurons in the ventral tier of the substantia nigra pars compacta (SNc) degenerate prominently in Parkinson’s disease (PD), while those in the dorsal tier and ventral tegmental area are relatively spared. The factors determining why these neurons are more vulnerable than others are still unrevealed. Neuroinflammation and immune cell infiltration have been demonstrated to be a key feature of neurodegeneration in PD. However, the link between selective dopaminergic neuron vulnerability, glial and immune cell response, and vascularization and their interactions has not been deciphered. We aimed to investigate the contribution of glial cell activation and immune cell infiltration in the selective vulnerability of ventral dopaminergic neurons within the midbrain in a non-human primate model of PD. Structural characteristics of the vasculature within specific regions of the midbrain were also evaluated. Parkinsonian monkeys exhibited significant microglial and astroglial activation in the whole midbrain, but no major sub-regional differences were observed. Remarkably, the ventral substantia nigra was found to be typically more vascularized compared to other regions. This feature might play some role in making this region more susceptible to immune cell infiltration under pathological conditions, as greater infiltration of both T- and B- lymphocytes was observed in parkinsonian monkeys. Higher vascular density within the ventral region of the SNc may be a relevant factor for differential vulnerability of dopaminergic neurons in the midbrain. The increased infiltration of T- and B- cells in this region, alongside other molecules or toxins, may also contribute to the susceptibility of dopaminergic neurons in PD.
Chromosomal-scale genome assembly of the Mediterranean mussel Mytilus galloprovincialis
Authors: Han Guo-dong , Ma Dan-dan , Du Li-na , Zhao Zhen-jun
Published: June 2024

The Mediterranean mussel, Mytilus galloprovincialis, is a significant marine bivalve species that has ecological and economic importance. This species is robustly resilient and highly invasive. Despite the scientific and commercial interest in studying its biology and aquaculture, there remains a need for a high-quality, chromosome-scale reference genome. In this study, we have assembled a high-quality chromosome-scale reference genome for M. galloprovincialis. The total length of our reference genome is 1.41 Gb, with a scaffold N50 sequence length of 96.9 Mb. BUSCO analysis revealed a 97.5% completeness based on complete BUSCOs. Compared to the four other available M. galloprovincialis assemblies, the assembly described here is dramatically improved in both contiguity and completeness. This new reference genome will greatly contribute to a deeper understanding of the resilience and invasiveness of M. galloprovincialis.
Plant interactions can lead to emergent relationships between plant community diversity, productivity and vulnerability to invasion
Authors: Trevenen Elizabeth J. , Veneklaas Erik J. , Teste François P. , Dobrowolski Mark P. , Mucina Ladislav , Renton Michael
Published: June 2024

Understanding what makes a community vulnerable to invasion is integral to the successful management of invasive species. Our understanding of how characteristics of resident plant interactions, such as the network architecture of interactions, can affect the invasibility of plant communities is limited. Using a simulation model, we tested how successfully a new plant invader established in communities with different network architectures of species interactions. We also investigated whether species interaction networks lead to relationships between invasibility and other community properties also affected by species interaction networks, such as diversity, species dominance, compositional stability and the productivity of the resident community. We found that higher invasibility strongly related with a lower productivity of the resident community. Plant interaction networks influenced diversity and invasibility in ways that led to complex but clear relationships between the two. Heterospecific interactions that increased diversity tended to decrease invasibility. Negative conspecific interactions always increased diversity and invasibility, but increased invasibility more when they increased diversity less. This study provides new theoretical insights into the effects of plant interaction networks on community invasibility and relationships between diversity and invasibility. Combined with increasing empirical evidence, these insights could have useful implications for the management of invasive plant species.
Geographic origin, ancestry, and death circumstances at the Cornaux/Les Sauges Iron Age bridge, Switzerland
Authors: Laffranchi Zita , Zingale Stefania , Indra Lara , Coia Valentina , Salazar García Domingo C. , Paladin Alice , Kaeser Marc-Antoine , Delley Géraldine , Szidat Sönke , Lösch Sandra , Zink Albert , Milella Marco
Published: June 2024

Cornaux/Les Sauges (Switzerland, Late Iron Age) revealed remnants of a wooden bridge, artifacts, and human and animal skeletal remains. The relationship between the collapsed structure and the skeletal material, whether it indicates a potential accident or cultural practices, remains elusive. We evaluate the most plausible scenario for Cornaux based on osteological, taphonomic, isotopic, and paleogenomic analysis of the recovered individuals. The latter amount to at least 20 individuals, mostly adult males. Perimortem lesions include only blunt force traumas. Radiocarbon data fall between the 3rd and 1st c. BCE, although in some cases predating available dendrochronological estimates from the bridge. Isotopic data highlight five to eight nonlocals. No close genetic relatedness links the analyzed skeletons. Paleogenomic results, the first for Iron Age Switzerland, point to a genetic affinity with other Central and Western European Iron Age groups. The type of skeletal lesions supports an accidental event as the more plausible explanation. Radiocarbon data and the demographic structure of the sample may suggest a sequence of different events possibly including executions and/or sacrifices. Isotopic and paleogenomic data, while not favoring one scenario over the other, do support earlier interpretations of the last centuries BCE in Europe as a dynamic period from a biocultural perspective.
Exploring affinity between organic probes and Prussian Blue Analogues via inverse gas chromatography
Authors: Paulusma Stijn , Singh Kaustub , Smeding Tom , Gamaethiralalage Jayaruwan G. , Claassen Frank W. , Beijleveld Hans , Janssen Hans-Gerd , de Smet Louis C. P. M.
Published: June 2024

Prussian Blue Analogues (PBAs), which are characterized by their open structure, high stability, and non-toxic properties, have recently been the subject of research for various applications, including their use as electrode precursors for capacitive deionization, gas storage, and environmental purification. These materials can be readily tailored to enhance their affinity towards gases for integration with sensing devices. An improved understanding of PBA-gas interactions is expected to enhance material development and existing sensor deposition schemes greatly. The use of inverse gas chromatography (IGC) is a robust approach for examining the relationship between porous materials and gases. In this study, the adsorption properties of (functionalized) hydrocarbons, i.e., probe molecules, on the copper hexacyanoferrate (CuHCF) lattice were studied via IGC, demonstrating that alkylbenzenes have a higher affinity for this material than n-alkanes. This difference was rationalized by steric hindrance, π–π interactions, and vapour pressure effects. Along the same line, the five isomers of hexane showed decreasing selectivity upon increased steric hindrance. Enthalpy values for n-pentane, n-hexane and n-heptane were lower than that of toluene. The introduction of increased probe masses resulted in a surface coverage of 46% for toluene. For all n-alkane probe molecules this percentage was lower. However, the isotherms of these probes did not show saturation points and the observed linear regime proves beneficial for gas sensing. Our work demonstrates the versatility of CuHCF for gas sensing purposes and the potential of IGC to characterize the adsorption characteristics of such a porous nanomaterial.
Implementation of theoretical non-photochemical quenching (NPQ(T)) to investigate NPQ of chickpea under drought stress with High-throughput Phenotyping
Authors: Lauterberg Madita , Tschiersch Henning , Zhao Yusheng , Kuhlmann Markus , Mücke Ingo , Papa Roberto , Bitocchi Elena , Neumann Kerstin
Published: June 2024

Non-photochemical quenching (NPQ) is a protective mechanism for dissipating excess energy generated during photosynthesis in the form of heat. The accelerated relaxation of the NPQ in fluctuating light can lead to an increase in the yield and dry matter productivity of crops. Since the measurement of NPQ is time-consuming and requires specific light conditions, theoretical NPQ (NPQ(T)) was introduced for rapid estimation, which could be suitable for High-throughput Phenotyping. We investigated the potential of NPQ(T) to be used for testing plant genetic resources of chickpea under drought stress with non-invasive High-throughput Phenotyping complemented with yield traits. Besides a high correlation between the hundred-seed-weight and the Estimated Biovolume, significant differences were observed between the two types of chickpea desi and kabuli for Estimated Biovolume and NPQ(T). Desi was able to maintain the Estimated Biovolume significantly better under drought stress. One reason could be the effective dissipation of excess excitation energy in photosystem II, which can be efficiently measured as NPQ(T). Screening of plant genetic resources for photosynthetic performance could take pre-breeding to a higher level and can be implemented in a variety of studies, such as here with drought stress or under fluctuating light in a High-throughput Phenotyping manner using NPQ(T).
The matricellular protein CCN5 prevents anti-VEGF drug-induced epithelial-mesenchymal transition of retinal pigment epithelium
Authors: Im Sora , Song Min Ho , Elangovan Muthukumar , Woo Kee Min , Park Woo Jin
Published: June 2024

Age-related macular degeneration (AMD) is one of the major causes of blindness in the elderly worldwide. Anti-vascular endothelial growth factor (VEGF) drugs have been widely used to treat the neovascular type of AMD (nAMD). However, VEGF acts not only as a pro-angiogenic factor but also as an anti-apoptotic factor in the eyes. In this study, we found that anti-VEGF drugs, including bevacizumab (Bev), ranibizumab (Ran), and aflibercept (Afl), induced epithelial-mesenchymal transition (EMT) in ARPE-19 cells in vitro, accompanied by the induction of CCN2, a potent pro-fibrotic factor. Similarly, intravitreal injection of Afl into mouse eyes resulted in EMT in the retinal pigmented epithelium (RPE). Co-treatment with CCN5, an anti-fibrotic factor that down-regulates CCN2 expression, significantly attenuated the adverse effects of the anti-VEGF drugs both in vitro and in vivo. Inhibition of the VEGF signaling pathway with antagonists of VEGF receptors, SU5416 and ZM323881, induced EMT and up-regulated CCN2 in ARPE-19 cells. Additionally, knock-down of CCN2 with siRNA abolished the adverse effects of the anti-VEGF drugs in ARPE-19 cells. Collectively, these results suggest that anti-VEGF drugs induce EMT in RPE through the induction of CCN2 and that co-treatment with CCN5 attenuates the adverse effects of anti-VEGF drugs in mouse eyes.
A dilute sodium hydroxide technique for radiolarian extraction from cherts
Authors: Onoue Tetsuji , Hori Sakiko , Tomimatsu Yuki , Rigo Manuel
Published: June 2024

Radiolarians have been used to determine geological ages and have contributed markedly to our understanding of Earth’s history. Hydrofluoric acid (HF) has traditionally been used to extract radiolarian fossils from siliceous deposits (i.e., radiolarian cherts), but this acid is strictly regulated because of environmental and human health concerns. Here we report on the successful extraction of radiolarians from cherts using a low-concentration NaOH solution (1 mol/L NaOH) as an alternative to HF. The degree of chert dissolution in NaOH is strongly temperature-dependent and is limited at < 80 °C. However, even a 1 mol/L NaOH solution is sufficient to dissolve chert at 100 °C. Our new NaOH method yields better-preserved radiolarian fossils compared with the conventional HF method. The 1 mol/L NaOH solution is less hazardous, easier to handle, and has fewer effects on the environment and human health than HF. Therefore, this method can be widely used for research and teaching purposes in studies of radiolarian fossils, even in institutions where HF cannot be used owing to chemical restrictions.
In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells
Authors: Jado Juan Carlos , Dow Michelle , Carolino Krypton , Klie Adam , Fonseca Gregory J. , Ideker Trey , Carter Hannah , Winzeler Elizabeth A.
Published: June 2024

In vitro evolution and whole genome analysis has proven to be a powerful method for studying the mechanism of action of small molecules in many haploid microbes but has generally not been applied to human cell lines in part because their diploid state complicates the identification of variants that confer drug resistance. To determine if haploid human cells could be used in MOA studies, we evolved resistance to five different anticancer drugs (doxorubicin, gemcitabine, etoposide, topotecan, and paclitaxel) using a near-haploid cell line (HAP1) and then analyzed the genomes of the drug resistant clones, developing a bioinformatic pipeline that involved filtering for high frequency alleles predicted to change protein sequence, or alleles which appeared in the same gene for multiple independent selections with the same compound. Applying the filter to sequences from 28 drug resistant clones identified a set of 21 genes which was strongly enriched for known resistance genes or known drug targets (TOP1, TOP2A, DCK, WDR33, SLCO3A1). In addition, some lines carried structural variants that encompassed additional known resistance genes (ABCB1, WWOX and RRM1). Gene expression knockdown and knockout experiments of 10 validation targets showed a high degree of specificity and accuracy in our calls and demonstrates that the same drug resistance mechanisms found in diverse clinical samples can be evolved, discovered and studied in an isogenic background.
CACSNet for automatic robust classification and segmentation of carotid artery calcification on panoramic radiographs using a cascaded deep learning network
Authors: Yoo Suh-Woo , Yang Su , Kim Jo-Eun , Huh Kyung-Hoe , Lee Sam-Sun , Heo Min-Suk , Yi Won-Jin
Published: June 2024

Stroke is one of the major causes of death worldwide, and is closely associated with atherosclerosis of the carotid artery. Panoramic radiographs (PRs) are routinely used in dental practice, and can be used to visualize carotid artery calcification (CAC). The purpose of this study was to automatically and robustly classify and segment CACs with large variations in size, shape, and location, and those overlapping with anatomical structures based on deep learning analysis of PRs. We developed a cascaded deep learning network (CACSNet) consisting of classification and segmentation networks for CACs on PRs. This network was trained on ground truth data accurately determined with reference to CT images using the Tversky loss function with optimized weights by balancing between precision and recall. CACSNet with EfficientNet-B4 achieved an AUC of 0.996, accuracy of 0.985, sensitivity of 0.980, and specificity of 0.988 in classification for normal or abnormal PRs. Segmentation performances for CAC lesions were 0.595 for the Jaccard index, 0.722 for the Dice similarity coefficient, 0.749 for precision, and 0.756 for recall. Our network demonstrated superior classification performance to previous methods based on PRs, and had comparable segmentation performance to studies based on other imaging modalities. Therefore, CACSNet can be used for robust classification and segmentation of CAC lesions that are morphologically variable and overlap with surrounding structures over the entire posterior inferior region of the mandibular angle on PRs.
Centaurea behen leaf extract mediated green synthesized silver nanoparticles as antibacterial and removing agent of environmental pollutants with blood compatible and hemostatic effects
Authors: Abdoli Mohadese , Khaledian Salar , Mavaei Maryamosadat , Hajmomeni Pouria , Ghowsi Mahnaz , Qalekhani Farshad , Nemati Houshang , Fattahi Ali , Sadrjavadi Komail
Published: June 2024

The present study focused on evaluating the antibacterial properties, radical scavenging, and photocatalytic activities of Centaurea behen-mediated silver nanoparticles (Cb-AgNPs). The formation of Cb-AgNPs was approved by UV–Vis spectrometry, Fourier-transform infrared spectroscopy, transmission electron microscopy, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. The results showed that the obtained AgNPs have a maximum absorbance peak at 450 nm with spherical morphology and an average size of 13.03 ± 5.8 nm. The catalytic activity of the Cb-AgNPs was investigated using Safranin O (SO) solution as a cationic dye model. The Cb-AgNPs performed well in the removal of SO. The coupled physical adsorption/photocatalysis reaction calculated about 68% and 98% degradation of SO dye under solar irradiation. The Cb-AgNPs inhibited the growth of gram-negative or positive bacteria strains and had excellent DPPH radicals scavenging ability (100% in a concentration of 200 µg/ml) as well as a good effect on reducing coagulation time (at concentrations of 200 and 500 µg/mL reduced clotting time up to 3 min). Considering the fact that green synthesized Cb-AgNPs have antioxidant and antibacterial properties and have a good ability to reduce coagulation time, they can be used in wound dressings. As well as these NPs with good photocatalytic activity can be a suitable option for degrading organic pollutants.
Elucidating the function of STING in systemic lupus erythematosus through the STING Goldenticket mouse mutant
Authors: Vejvisithsakul Pichpisith Pierre , Thumarat Chisanu , Leelahavanichkul Asada , Hirankan Nattiya , Pisitkun Trairak , Paludan Soren Riis , Pisitkun Prapaporn
Published: June 2024

The complexity of systemic lupus erythematosus (SLE) arises from intricate genetic and environmental interactions, with STING playing a pivotal role. This study aims to comprehend the function of STING using the pristane-induced lupus (PIL) model in Sting missense mutant mice (Goldenticket or StingGt), which contrasts with previous research using Sting knockout mice. Investigating two-month-old StingGt mice over six months post-PIL induction, we observed a profound reduction in autoimmune markers, including antinuclear and anti-dsDNA antibodies, germinal center B cells, and plasma cells, compared to their wild-type counterparts. A pivotal finding was the marked decrease in IL-17-producing T cells. Notably, the severity of lupus nephritis and pulmonary hemorrhages was significantly diminished in StingGt mice. These findings demonstrate that different genetic approaches to interfere with STING signaling can lead to contrasting outcomes in SLE pathogenesis, which highlights the need for a nuanced understanding of the role of STING in drug development for SLE. In summary, the loss of Sting function in Goldenticket mutant mice rescued autoimmune phenotypes in PIL. This study reveals the critical nature of STING in SLE, suggesting that the method of STING modulation significantly influences disease phenotypes and should be a key consideration in developing targeted therapies.
N-butyl-β-carboline-3-carboxylate (β-CCB) systemic administration promotes remyelination in the cuprizone demyelinating model in mice
Authors: Vélez-Uriza Fidel , Ordaz Rainald Pablo , Garay Edith , Cisneros-Mejorado Abraham J. , Arellano Rogelio O.
Published: June 2024

Demyelination is generated in several nervous system illnesses. Developing strategies for effective clinical treatments requires the discovery of promyelinating drugs. Increased GABAergic signaling through γ-aminobutyric acid type A receptor (GABAAR) activation in oligodendrocytes has been proposed as a promyelinating condition. GABAAR expressed in oligodendroglia is strongly potentiated by n-butyl-β-carboline-3-carboxylate (β-CCB) compared to that in neurons. Here, mice were subjected to 0.3% cuprizone (CPZ) added in the food to induce central nervous system demyelination, a well-known model for multiple sclerosis. Then β-CCB (1 mg/Kg) was systemically administered to analyze the remyelination status in white and gray matter areas. Myelin content was evaluated using Black-Gold II (BGII) staining, immunofluorescence (IF), and magnetic resonance imaging (MRI). Evidence indicates that β-CCB treatment of CPZ-demyelinated animals promoted remyelination in several white matter structures, such as the fimbria, corpus callosum, internal capsule, and cerebellar peduncles. Moreover, using IF, it was observed that CPZ intake induced an increase in NG2+ and a decrease in CC1+ cell populations, alterations that were importantly retrieved by β-CCB treatment. Thus, the promyelinating character of β-CCB was confirmed in a generalized demyelination model, strengthening the idea that it has clinical potential as a therapeutic drug.
Calibration of contact parameters of sandy soil for planting tiger nut based on non-linear tools
Authors: Qi Jiangtao , Chen Sirui , Yang Luoyi , An Shiguan , Meng Hewei , Kan Za
Published: June 2024

A methodology combining physical experiments with simulation was employed to acquire contact parameters of sandy soil precisely for planting tiger nuts in the desert area of Xinjiang. The stacking angle under different parameter combinations was applied as a response value. Through the Plackett–Burman test, several factors that have a significant influence were determined. The steepest ascent test was conducted to establish the finest scope of values for these parameters. The stacking angle was considered the response variable, and non-linear tools were used to optimize these parameters for simulation. The findings showed that applying response surface methodology (RSM) resulted in a relative error of 1.24%. In the case of BP-GA, the relative error compared to the physical test value was 0.34%, while for BP, it was 2.18%. After optimization using Wavelet Neural Network (WNN), the relative error was reduced to only 0.15%. Results suggest that WNN outperforms the RSM model, and the sandy soil model and parameters generated using WNN can be effectively utilized for discrete element simulation research.
RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31
Authors: Tian Wei , Zhao Jingyuan , Zhang Xinyu , Li Pengfei , Li Xuening , Hong Yuan , Li Shuai
Published: June 2024

Colon adenocarcinoma (COAD) is the second leading cause of cancer death, and there is still a lack of diagnostic biomarkers and therapeutic targets. In this study, bioinformatics analysis of the TCGA database was used to obtain RUNX1, a gene with prognostic value in COAD. RUNX1 plays an important role in many malignancies, and its molecular regulatory mechanisms in COAD remain to be fully understood. To explore the physiological role of RUNX1, we performed functional analyses, such as CCK-8, colony formation and migration assays. In addition, we investigated the underlying mechanisms using transcriptome sequencing and chromatin immunoprecipitation assays. RUNX1 is highly expressed in COAD patients and significantly correlates with survival. Silencing of RUNX1 significantly slowed down the proliferation and migratory capacity of COAD cells. Furthermore, we demonstrate that CDC20 and MCM2 may be target genes of RUNX1, and that RUNX1 may be physically linked to the deubiquitinating enzyme USP31, which mediates the upregulation of RUNX1 protein to promote transcriptional function. Our results may provide new insights into the mechanism of action of RUNX1 in COAD and reveal potential therapeutic targets for this disease.
Regulatory network analysis based on integrated miRNA-TF reveals key genes in heart failure
Authors: Zhang Ziyue , Zou Ziying , Zhang Hui , Zhang Dai-Min
Published: June 2024

The etiology and pathophysiology of heart failure are still unknown. Increasing evidence suggests that abnormal microRNAs (miRNAs) and transcription factors (TFs) expression may be associated with the development of heart failure. Therefore, this study aims to explore key miRNAs, TFs, and related genes in heart failure to gain a greater understanding of the pathogenesis of heart failure. To search and download the dataset of mRNA chips related to heart failure from the GEO database (GSE59867, GSE9128, and GSE134766), we analyzed differential genes and screened the common differentially expressed genes on two chips using R language software. The binary interactions and circuits among miRNAs, TFs, and corresponding genes were determined by Pearson correlation coefficient. A regulatory network of miRNAs, TFs, and target genes was constructed based on bioinformatics. By comparing the sequences of patients with and without heart failure, five downregulated genes with hypermethylated mRNA and three upregulated genes with hypomethylated mRNA were identified. The miRNA-TF gene regulatory network consisted of 26 miRNAs, 22 TFs and six genes. GO and KEGG analysis results revealed that BP terms like cellular response to organic substance, cellular response to cytokine stimulus, and KEGG pathways like osteoclast differentiation, MAPK signaling pathway, and legionellosis were enriched of the DEGs. TMEM87A, PPP2R2A, DUSP1, and miR-92a have great potential as biomarkers for heart failure. The integrated analysis of the mRNA expression spectrum and microRNA-transcription factor-gene revealed the regulatory network of heart failure, which may provide clues to its alternative treatment.
Modulation of allograft immune responses by Porphyromonas gingivalis lipopolysaccharide administration in a rat model of kidney transplantation
Authors: Sato Yu , Noguchi Hiroshi , Kubo Shinsuke , Kaku Keizo , Okabe Yasuhiro , Onishi Hideya , Nakamura Masafumi
Published: June 2024

Periodontitis is a chronic inflammatory disease that affects the periodontal tissues. Although it is associated with various systemic diseases, the impact of periodontitis on kidney transplantation (KT) outcomes, particularly allograft rejection, remains unclear. This study investigated the effect of periodontitis on transplant immunity, specifically examining Porphyromonas gingivalis-derived lipopolysaccharide (LPS-PG). In vitro experiments revealed that LPS-PG increased regulatory T cells (Tregs) in Lewis rat spleen cells. In a mixed lymphocyte reaction assay, concentrations of interferon-γ, indicative of alloreactivity, were lower than in controls when LPS-PG was added to the culture and when LPS-PG-administered Lewis rat spleen cells were used as responders. In a rat KT model, LPS-PG administration to recipients promoted mild tubulitis and low serum creatinine and blood urea nitrogen levels 5 days post-KT compared with PBS-administered controls. Furthermore, LPS-PG-administered recipients had an elevated Treg proportion in their peripheral blood and spleen cells, and increased infiltrating Tregs in kidney allografts, compared with controls. The elevated Treg proportion in peripheral blood and spleen cells had a significant negative correlation with serum creatinine, suggesting elevated Tregs modulated allograft rejection. These findings suggest that periodontitis might modulate alloimmune reactivity through LPS-PG and Tregs, offering insights to refine immunosuppressive strategies for KT recipients.
3D-printed ultra-small Brownian viscometers
Authors: Vizsnyiczai Gaszton , Kubacková Jana , Iványi Gergely T. , Slabý Cyril , Horváth Denis , Hovan Andrej , Strejčková Alena , Tomori Zoltán , Kelemen Lóránd , Bánó Gregor
Published: June 2024

Measuring viscosity in volumes smaller than a microliter is a challenging endeavor. A new type of microscopic viscometers is presented to assess the viscosity of Newtonian liquids. Micron-sized flexible polymer cantilevers are created by two-photon polymerization direct laser writing. Because of the low stiffness and high elasticity of the polymer material the microcantilevers exhibit pronounced Brownian motion when submerged in a liquid medium. By imaging the cantilever’s spherically shaped end, these fluctuations can be tracked with high accuracy. The hydrodynamic resistance of the microviscometer is determined by fitting the power spectral density of the measured fluctuations with a theoretical frequency dependence. Validation measurements in water-glycerol mixtures with known viscosities reveal excellent linearity of the hydrodynamic resistance to viscosity, allowing for a simple linear calibration. The stand-alone viscometer structures have a characteristic size of a few tens of microns and only require a very basic external instrumentation in the form of microscopic imaging at moderate framerates (~ 100 fps). Thus, our results point to a practical and simple to use ultra-low volume viscometer that can be integrated into lab-on-a-chip devices.
Specialized diving traits in the generalist morphology of Fulica (Aves, Rallidae)
Authors: De Mendoza Ricardo Santiago , Carril Julieta , Degrange Federico Javier , Tambussi Claudia Patricia
Published: June 2024

Foot-propelled diving comprises the primary locomotion-based feeding strategy for many birds, including families such as Phalacrocoracidae, Anhingidae, Podicipedidae, Gaviidae, and the diving ducks within Anatidae. While the morphology of specialized divers is well known, the corresponding morphology is less known for birds not as specialized but capable of diving, such as the coots (Rallidae, Fulica spp.). To compare the osteology of Fulica with other (non-diving) Rallidae, and with foot-propelled diving birds that are distantly related, we considered osteological characters, as well as the proportion of the hind limb bones and the femoral splay angle to construct a phylomorphospace, and to perform a comparative disparity analysis considering ecomorphologically relevant characters related to swimming and diving. Coots resulted to be significantly disparate from other Rallidae showing many traits of specialized foot-propelled divers, but only noticeable when compared with other rallids, as the degree of development of these traits is markedly less than in loons, grebes, or cormorants. This may correspond to a stabilizing selection of characteristics associated with a generalist morphology in Fulica. Studying adaptation in generalist taxa broadens our understanding of ecomorphologically significant features, thereby enabling us to generalize their evolutionary patterns.