bluuebunny/crossref_metadata_2025 huggingface dataset:
Pain intensity and stress indicators after cemented total knee replacement with epidural application of morphine: prospective randomized study
Authors: Sokolov S. V. , Glushchenko V. A.
Published: January 2025

The objective to perform the objective assessment of the intensity of pain syndrome after cemented total knee replacement with epidural analgesia with 0.2% ropivacaine solution and epidural analgesia with a combination of 0.2% ropivacaine solution with morphine was performed.

Materials and methods. The study included 60 patients who underwent cemented total knee replacement for gonarthrosis under combined spinal-epidural anesthesia. In patients of the control group (n = 30), postoperative anesthesia was performed with 0.2% ropivacaine solution epidural through a catheter at the L2–3–L1–2 level in the form of a bolus followed by constant infusion. In patients of the main group (n = 30), analgesia was performed according to the same technique, but using a morphine solution of 1% – 0.3 ml (3mg) as part of a bolus of 0.2% ropivacaine solution epidural. The hemodynamics of the patients, the volume of intraoperative blood loss, and infusion therapy were homogeneous due to the carefully developed surgical procedure, had no significant differences and were not taken into account when publishing the data. The level of glycemia and cortisol in venous blood on the day of surgery (1 hour before surgery and 4 hours after surgery), age, and pain intensity on a numerical rating scale (NRS) were studied. Statistical processing was performed by MedCalc Software Ltd.

Results. In the postoperative period, the level of venous blood cortisol in patients of the main group was 486.2 [470.6; 494.5] nmol/l, and in patients of the control group – 876.8 [803.7; 918.7] nmol/l (p < 0.001 according to the Mann–Whitney U-criterion); pain intensity on the numerical rating scale in the main group was 1 [1; 1.5] score, in the control group was 4 [3; 5] scores (p < 0.001, U–Mann–Whitney criterion). There was also a correlation between the value of glycemia and the pain intensity by NRS in the postoperative period (Spearman’s coefficient r = 0.669, 95% CI = 0.499–0.789, p < 0.0001).

Conclusions. As a result of the study, it was found that epidural analgesia with morphine leads to a lower increase in glucose and cortisol levels in the postoperative period, lower pain intensity when subjectively assessed using the numerical rating scale, which indicates a high quality of anesthesia.
Arterial pressure during first day after intravenous thrombolysis and acute ischemic stroke outcomes: single-center randomized controlled study
Authors: Avidzba A. R. , Saskin V. A. , Nikonov A. M. , Ayyaz H. , Kirov M. Yu.
Published: January 2025

Introduction. Blood pressure optimization during and after reperfusion in patients with acute ischemic stroke can reduce the risk of hemorrhagic complications and improve functional recovery. Several randomized controlled trials of different target blood pressure values have been published with varying results.

The objective was to evaluate the effect of intensive hypotensive therapy in the first day after intravenous thrombolysis on outcomes of acute ischemic stroke and incidence of complications.

Materials and methods. A single-center open label randomized controlled trial was conducted. Patients older than 18 years with acute ischemic stroke who underwent reperfusion were included. Patients were randomized into 2 groups: with a target systolic blood pressure of 161–185 mm Hg on the first day (control group) versus < 160 mm Hg (intensive hypotensive therapy group). The primary end points were mortality and modified Rankin Scale score at day 90 from the onset of stroke.

Results. The final analysis included 69 patients. In the intensive hypotensive therapy group, mortality rate did not differ from the control group: OR 1.1 [95% CI 0.3 to 4.8] (p = 0.896). The median modified Rankin scale score at day 90 in the control group was 2 (1; 3.8) versus 2 (1; 3.5) in the intensive hypotensive therapy group (p = 0.812).

Conclusion. Intensive hypotensive therapy at the first day after intravenous thrombolysis in acute ischemic stroke patients with target values of systolic blood pressure < 160 mm Hg compared to conventional values of 161–185 mm Hg neither improved functional outcome nor decreased mortality and complications rate on day 90 from the onset of stroke.
Septic shock in the intensive care unit of a multidisciplinary hospital
Authors: Plotkin L. L. , Artamonov V. A.
Published: January 2025

The objective of the study was to assess the prevalence, etiology, and outcomes of intensive care in patients with septic shock in the ICU of a multidisciplinary hospital.

Materials and methods. A single-center, retrospective, cohort study was conducted involving 398 patients with septic shock who were hospitalized in the ICU over a one-year period. Diagnosis of septic shock, as well as a complex of intensive care, were carried out according to the criteria proposed by the recommendations of the Surviving sepsis campaign (2021). Antibacterial therapy was prescribed based on the Guidelines «Diagnosis and antimicrobial therapy of infections caused by multidrug-resistant strains of microorganisms» (2022)

Results. The proportion of patients with septic shock was 7.4% of the entire population hospitalized in the ICU. The hospital mortality rate was 25%. The dominant loci of infection in patients with septic shock were the abdominal cavity (33%) and lungs (32.4%). The microbial landscape was dominated by gram-negative flora (66%), among which in 35% of cases, Klebsiella pneumoniae was characterized by resistance to the antibacterial drugs used. Combined antibacterial therapy was received by 84% of patients.
Information technologies and key components for ensuring patient safety in anesthesiology and intensive care units with different levels of digitalization (message 3)
Authors: Gorban V. I.
Published: January 2025

The objective was to assess the relationship between the implementation of information technologies in the work of anesthesiology and intensive care services of multidisciplinary hospitals and the severity of the leading components of patient safety at different levels of digitalization.

Materials and methods. Expert assessment of the level of digitalization and the effectiveness of using new medical information technologies in the system of the anesthesiology and intensive care service (department) of 235 multidisciplinary hospitals, including an assessment of the leading components of patient safety at different levels of digitalization. Statistical analysis of data distribution, Student’s t-test for independent samples.

Result. It has been established that the majority (47.7%) of anesthesiology and intensive care units (AICU) of multidisciplinary hospitals in the Russian Federation are at the average (sufficient) level of digitalization. Relatively few (19.2%) AICU of multidisciplinary hospitals are at a high and sufficiently high level in terms of digitalization. At the same time, each of the levels of digitalization of the AICU of multidisciplinary hospitals has a close relationship with the severity of the main components of ensuring the safety of patients in the departments.

Conclusion. Comparison (correlation) of the level of digitalization and components of patient safety of the AICU of multidisciplinary hospitals showed their fairly close and direct relationship. The study confirms that the effectiveness of ensuring patient safety of the AICU of multidisciplinary hospitals is associated, first of all, with a high level of digitalization as an important integral process for ensuring the quality of specialized medical care.
The role of regional anesthesia techniques in the treatment of lumbosacral plexopathy after aorto-femoral bifurcation prosthetics (clinical case)
Authors: Paromov K. V. , Svirskii D. A. , Neledova L. A. , Kirov M. Yu.
Published: January 2025

The objective was to demonstrate the effectiveness of regional anesthesia techniques in the treatment of ischemic lumbosacral plexopathy after vascular surgery.

Introduction. The frequency of ischemic complications after infrarenal aortic surgery is less than 1%. The persistence and severity of neurological symptoms, as well as the low effectiveness of therapy, can negatively affect the result of surgical intervention.

Materials and methods. A 66-year-old patient on the first day after an elective simultaneous aorto-femoral bifurcation prosthetics and cholecystectomy due to an aneurysm of the infrarenal aorta and calculous cholecystitis under inhalation anesthesia had symptoms of left-sided lumbosacral plexopathy, manifesting with left-sided inferior monoplegia, mosaic sensitivity disorders and severe pain syndrome.

Results. During routine pain therapy (acetaminophen, nonsteroidal anti-inflammatory drugs, opioids), on the second day after surgery, a transverse abdominis plane block (TAP-block) on the right side and pericapsular nerve group block (PENG-block) on the left side were performed, that led to a rapid regression of acute postoperative pain and restoration of patient mobility. On the 7th, 11th and 16th days after surgery, neurotrophic therapy and comprehensive rehabilitation were supplemented with interventional pain therapy techniques, which provided not only effective pain relief, but also rapid improvement of neurological symptoms. On the 22nd day of postoperative period, the patient was discharged from the hospital with slight motor deficit in the left leg.

Conclusions. The case describes complicated course of the postoperative period after aorto-femoral bifurcation prosthetics and cholecystectomy and effectiveness of the interventional therapy for lumbosacral plexopathy of ischemic origin. This strategy led to a rapid regression of neurological deficit in parallel with relief of acute postoperative pain and expansion of rehabilitation opportunities.
Evaluation of the efficacy of enteral therapy in a patient with posttraumatic pancreatitis (clinical case)
Authors: Zhigalova M. S. , Kiselev V. V. , Petrikov S. S. , Yartsev P. A. , Ryk A. A. , Teterin Yu. S. , Shavrina N. V. , Selina I. E. , Rogal M. M. , Staleva K. V.
Published: January 2025

Introduction. In the development of post-traumatic pancreatitis, an important role is played by microcirculatory disorders, developing against the background of inflammation and decreased capillary blood flow not only in the tissues of the pancreas, but also in other organs, including the intestine, which leads to neuroendocrine dysregulation, dysfunction and/or death of intestinal epithelial cells, disruption of the motor and evacuation functions of the intestine, and, as a consequence, the development of intestinal insufficiency syndrome.

The objective was to demonstrate the effectiveness of enteral therapy in a patient with a closed abdominal injury, liver injury and posttraumatic pancreatitis.

Materials and methods. We analyzed a clinical case of hospitalization of patient K., 38 years old, with the following diagnosis: “Closed abdominal trauma. Liver trauma of the S6 segment. Hemoperitoneum 1000 ml. Complication: right-sided segmental pneumonia. Bilateral hydrothorax. Posttraumatic pancreatitis”. The patient was admitted with complaints of upper abdominal pain and nausea two weeks after blunt abdominal trauma. She was treated as an outpatient. During the last two days she started to notice nausea, increased pain syndrome in the right side and upper parts. She was brought by an ambulance. Hybrid surgical intervention was performed: endovascular celiacography, superselective embolization of the right hepatic artery branch and diagnostic videolaparoscopic sanation and drainage of the abdominal cavity. In the postoperative period, treatment was performed according to the National Clinical Guidelines, supplemented with enteral therapy.

Conclusion. This clinical observation demonstrated the effectiveness of including enteral therapy in a complex of therapeutic measures in the patient with closed abdominal trauma, liver trauma, posttraumatic pancreatitis, which was expressed in a decrease in the level of lactate, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, C-reactive protein, leukocytes on the 3rd day of dynamic observation. At the same time, an increase in the absolute number of lymphocytes, the concentration of total protein and albumin was noted. The proposed scheme contributed to the regression of signs of intestinal insufficiency syndrome, normalization of intra-abdominal pressure by the 7th postoperative day, prevention of the development of purulent-septic complications and multiple organ failure, a successful outcome of hospitalization.
Teachers' knowledge about type-1 diabetes mellitus in primary schools in Jeddah, Saudi Arabia: a cross-sectional study
Authors: Almutairi Abdulaziz , Althobaiti Ahmed , Dawood Muhannad , Almalki Majed , Maruf Yahya , Bukhari Musab , Khojah Imad
Published: January 2025

Objective:
This study aimed to assess the level of knowledge among primary school teachers in Jeddah, Saudi Arabia, regarding type-1 diabetes mellitus (T1DM).
Methods:
A descriptive cross-sectional study was conducted from June to December 2022 among primary school teachers in Jeddah, Saudi Arabia. Data were gathered from 242 teachers using a validated, self-administered online questionnaire. The questionnaire covered various aspects, including participant demographics, experience with T1DM students, prior T1DM training, and 20 knowledge-based questions.
Results:
Most of the participants were females (63.2%), aged ≥40 years (76.4%), with work experience of more than 20 years (55%). Approximately two-thirds of the participants had never taught a student with T1DM before. However, only 30.2% reported school T1DM training programs within their school, and only 49 (20.2%) attended training in this field. The mean knowledge score was 12.92 (64.6%). Around one-third had poor knowledge (31.4 %), one-third had basic knowledge (36.8 %), and one-third had good knowledge sufficient to support T1DM students (31.8 %). The multivariate analysis explored factors associated with teachers' knowledge and found statistically insignificant (p>0.05) positive links with good knowledge.
Conclusions:
Primary school teachers in Jeddah had suboptimal T1DM knowledge. Specialized T1DM training programs, particularly for older teachers without T1DM experience, are needed to address knowledge gaps and better support children with T1DM.
COVID-19 infection induced health complications among sickle cell disease patients in the eastern province of Saudi Arabia
Authors: Fita Abdulrahman , Alhussain Abdullah , Subaih Abdulrahman , Alshehri Abdullah , Chathoth Shahanas , Cyrus Cyril
Published: January 2025

Objective:
The current study sought to assess the impact of COVID-19 on the worsening of sickle cell disease (SCD) complications and find long-term impacts that influence prognosis.
Methods:
A retrospective, questionnaire-based study included Saudi SCD patients with a history of COVID-19 infection. SCD patients without COVID-19 served as a control group. Clinical data were collected from hospital records and direct communication with patients.
Results:
Of 46 patients, 32.6% were aged 26–35 years, and 45.7% were females. Among 35 patients (76%) with COVID-19, 29 had a single infection, and 4 had two infections. Vaccination data showed 16 infections occurred pre-vaccination, six post-first dose, and seven post-second dose. Post-infection, predominant symptoms included abdominal pain (23%), shortness of breath (20%), and chest pain (17%). Acute chest syndrome-related complications occurred in 63% of cases, with 9% reporting stroke. Hydroxyurea (29 patients) and blood transfusions (17 patients) were common treatments, while 17.4% used daily painkillers.
Conclusion:
COVID-19 infection significantly increases the risk of acute chest syndrome and other complications in SCD patients. Long-term effects must be studied to improve timely and effective treatment strategies for this vulnerable population.
DESIGN, DEVELOPMENT AND CHARACTERIZATION OF EXTENDED RELEASE MULTIUNIT PARTICULATE SYSTEM OF ANTI-INFLAMMATORY DRUG
Authors: Daslaniya Dhiren , Patel Manish , Shah Amarish
Published: January 2025

Multi unit particulate system has long been employed to improve the bioavailability of drugs. Mesalamine pellets were prepared by Coating drug solution on sugar sphere followed by various functional coating. The influence of rate controlling membrane made up of Eudragit RSPO and Eudragit RLPO in combination with delay release polymer coating with Eudragit L100 in different proportions on drug release kinetics was studied. Pellets were for the various parameter like Physical characteristics, assay and in-vitro dissolution profile. The study confirmed that mesalamine can be delivered by multi unit particulate system into lower part of intestine. Optimized formulations were evaluated for In-vitro release profile. The optimized formula was stable at accelerated storage condition 40°C / 75 % RH. Prepared Pellets can be used in the treatment of the ulcerative colitis.
DESIGNING AND CHARACTERIZATION OF DRUG FREE PATCHES FOR TRANSDERMAL APPLICATION
Authors: Bharkatiya M. , Nema R. K. , Bhatnagar M.
Published: January 2025

The present investigation was taken up to prepare and evaluate drug free polymeric patches using different polymers and to study the effect of different plasticizers on physicochemical properties of the patches to explore their feasibility for transdermal application. Polyethylene glycol (PEG 400), Dibutylphthalate (DBP) and Propylene glycol (PG) were used as plasticizers at a concentration of 40 % w/w of dry polymer weight. Drug free polymeric patches were prepared by the casting method on  mercury surface and evaluated for weight variation, thickness, flatness, tensile strength, folding endurance, surface pH, hardness, swellability, water vapour transmission rate and skin irritation studies. The mercury substrate method was found to give thin uniform patches. 
The weight and thickness of the patches was found to be uniform. Tensile strength and folding endurance of the patches prepared with DBP as plasticizer was high compared to patches plasticized with PG and PEG. All the formulations show 100 % flatness. HPMC K4M: PVP patches plasticized with PEG 400 showed higher swellability and water vapour transmission rates. The patches were found to be free of any skin irritation. Based on the above observations, it can be reasonably concluded that plasticizers have a significant influence on the mechanical properties of the transdermal patches.
POLAROGRAPHIC DETERMINATION AND ANTIFUNGAL ACTIVITY OF CU (II) COMPLEX WITH 3-HYDROXY-3-M-TOLYL-1-P-(SULPHONAMIDO) PHENYLTRIAZENE
Authors: Joshi P. , Pareek N. , Upadhyay D. , Khanam R. , Bhandari A. , Chauhan RS , Goswami AK
Published: January 2025

The electrochemical behavior of complex of Cu (II) with 3-hydroxy-3-m-tolyl-1-p-(sulphonamido) phenyltriazene was studied. It was observed that HTSP forms 1:3 complex with Cu (II) in between pH 6.5 to 7.1. It was found that the reduction process of Cu (II) - HTSP complex is two electrons reversible reduction process at D.M.E. The logarithm value of stability constant of 1:3 Cu(II)-3-hydroxy-3-m-tolyl-1-p-(Sulphonamido) phenyltriazene complex has been found to be 11.63. The present paper also reports antifungal activity of hydroxytriazene 3-hydroxy-3-m-tolyl-1-(4-sulphonamidophenyl) triazene, and its Cu (II) complex against Rhizoctonia solani, the fungi causing disease in fennel. The result has been expressed as percentage inhibition (PI) of mycelial growth as well as sclerotium formation at 500 ppm for both ligand as well as complex. The result shows that PI value in complex is 87.03 whereas for ligand 79.63. Thus it is clear that complex is better in activity against the fungi compared to its respective ligand. The result has been interpreted in terms of theory of drug action proposed by Paton.
Identification of Degradation Pathway of Bilastine and Montelukast by LC/MS
Authors: Markanday Deepanshu , Sharma Chandra Shekhar
Published: January 2025

It is necessary for a dosage form to maintain their chemical, physical, microbiological and toxicological stability during their entire shelf life. Knowing the intrinsic stability of the chemical and the breakdown pathway of the dosage form is crucial for this reason.  We have developed a straightforward analytical approach using HPLC-UV/MS for the simultaneous measurement of Bilastine and Montelukast, as well as the identification of their degradants in tablet formulation. This method is specific, linear, accurate, and robust. The narcotic compounds were broken down one by one. The Zorbax XDB C-18 column (150*4.6) mm, 5µ, with a mobile phase of Water:Acetonitrile:Formic acid (50:50:1), was used to achieve the separation. A flow rate of 1 ml/min was used with an injection volume of 20 µl. A UV-Vis Spectrophotometer was used for peak detection, while a mass spectrophotometer was employed for identification. Bilastine had a retention time of 3.22 minutes and Montelukast of 4.80 minutes. Proposed method was stability indicating, specific, linear, accurate and robust. The degradation pathway was established for Bilastine and Montelukast by studying their degradation in various stress conditions. The Bilastine was found stable in Photolytic and thermal degradation while there was degradation observed in Montelukast in all stress conditions.
Development, validation and greenness assessment of stability indicating RP-HPLC method for simultaneous quantification of dapagliflozin and metoprolol succinate in synthetic mixture
Authors: Rathod Sapna , Shah Ayushi , Patel Nisarg
Published: January 2025

A novel, rapid, accurate, precise and simple stability indicating RP-HPLC method has been proposed and validated for the concurrent quantitation of Dapagliflozin and Metoprolol succinate in laboratory prepared mixture. The greenness assessment of the proposed approach was accomplished utilizing GAPI and AGREE tools. The final chromatographic run was performed utilizing Methanol: 20mM Potassium Dihydrogen Phosphate: (70:30) as eluent, injected at 25°C at a rate of 1.5 mL/min. Cosmosil-MS-5 C18 column (250 mm × 4.6 mm, 5 μm) was utilised and estimation was done using 223 nm. The APIs were exposed to stress environments (oxidation, acidic and basic hydrolysis, photolysis and thermal stimuli) and stressed samples were analysed under same chromatographic condition. Dapagliflozin and metoprolol succinate had retention times of 11.6 and 2.6 minutes, correspondingly. It was demonstrated having linearity within 10-30 µg/mL for Dapagliflozin and 50–150 µg/mL for Metoprolol succinate. The % accuracy for Dapagliflozin were in the range of 99.01 - 100.56 %, while for Metoprolol succinate it was within 99.32 - 101.41 % at three levels. The outcomes for precision study were within the limits. For Dapagliflozin, the LOD and LOQ were 0.49 µg/mL and 0.59 µg/mL correspondingly and for Metoprolol succinate, values were 1.48 µg/mL and 1.79 µg/mL correspondingly. The approach is effectively suggested for stability-indicating research as well as for routine estimation of Dapagliflozin and Metoprolol succinate in bulk and laboratory prepared mixture. Furthermore, AGREE and GAPI outcomes confirmed the developed approach’s eco–friendliness and greenness.
Spectroscopic Elucidation and Anticancer Potential of 7-methoxy-2-(4-methoxyphenyl)-5-O-glycosyl chromenone Isolated from Haplanthodes tentaculatus (L.) R. B. Majumdar
Authors: Trivedi Parth B , Badole Madhavi R
Published: January 2025

In this research, we report on the isolation and structural elucidation of a new flavonoid glycoside called 7-methoxy-2-(4-methoxyphenyl)-5-O-glycosyl chromenone. This flavonoid glycoside was obtained from Haplanthodes tentaculatus, a plant that is native to the Western Ghats of India. The molecule was purified via the use of chromatographic procedures, and its structure was validated by the use of spectroscopic investigations, which included HRMS, 1H NMR, 13C NMR, FTIR, and UV-Visive technologies. With the use of HRMS analysis, the chemical formula C₂₃H₂₄O₁₀ was verified. The isolated molecule demonstrated anticancer activity in vitro against the MDA-MB-231 breast cancer cell line, with an IC₅₀ value of 58.99 ± 0.495 µg/ml. This indicates that the drug is very effective in curing cancer. The natural origin of 7-methoxy-2-(4-methoxyphenyl)-5-O-glycosyl chromenone and the possibility that it has a reduced toxicity level imply that it has the potential to be a lead molecule for the creation of anticancer drugs, despite the fact that it is less effective than the conventional medicine Cisplatin. The findings of this research add to the increasing body of evidence that bioactive chemicals derived from natural sources have the potential to be used in therapeutic medicine.
Protective action of leaves of Epipremnum aureum in gastric ulcer prevention induced by ethanol in rats
Authors: Manasa S. , Cyriac Kusu Susan , Jha Deepak Kumar , R Raveena Shree
Published: January 2025

The study investigated the gastroprotective effects of Epipremnum aureum leaf extracts against ethanol-induced gastric ulcers in rats. Dosages of 200 mg/kg and 400 mg/kg of the methanolic extract were administered, with omeprazole used as the positive control. The methanol extract significantly protected the gastric mucosa from ethanol-induced damage, suggesting cytoprotective potential. Ethanol exposure led to a decrease in plasma antioxidant enzyme levels and increased lipid peroxidation, indicating oxidative stress. However, rats treated with the higher extract dose (400 mg/kg) and omeprazole showed significant improvements, with increased SOD and CAT levels, reduced lipid peroxidation, and a lower ulcer index compared to the untreated ulcer group. Histopathological analysis revealed severe gastric mucosal damage in the ethanol-only group, while the high-dose extract and omeprazole groups maintained normal gastric architecture with minimal inflammation. These findings suggest that Epipremnum aureum methanolic extract exhibits potent gastroprotective effects, potentially due to its rich phytochemical profile, including flavonoids, tannins, and alkaloids, supporting its use as a natural remedy for gastric ulcers.
To examine the potential effectiveness of Psidium guajava in Letrozole induced polycystic ovarian syndrome in female albino rats
Authors: R Raveena Shree , Jha Deepak Kumar
Published: January 2025

Polycystic ovarian syndrome (PCOS) is a common endocrine disorder characterized by hormonal imbalances, metabolic disturbances, and ovarian dysfunction. This study aimed to investigate the therapeutic potential of methanol extract from Psidium guajava leaves as a natural remedy for alleviating PCOS symptoms in female albino rats. The experimental approach involved assessing critical physiological and biochemical markers both before and after treatment, including body weight, blood levels of testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), ovarian weight, and the FSH/LH ratio. Anti-inflammatory effects were evaluated by measuring inflammatory markers like TNF-α and lipid-derived mediators. Furthermore, a histological analysis of ovarian tissue was performed to observe changes in follicular development and ovarian structure. The results showed that Psidium guajava treatment significantly improved hormonal profiles, reduced inflammation markers, and promoted the regeneration and maturation of ovarian follicles, counteracting hyperandrogenism and inflammation associated with PCOS. These findings suggest that Psidium guajava leaf extract could be a promising adjunct therapy for managing PCOS, supporting endocrine balance, ovarian health, and inflammation reduction. However, further studies are needed to understand the underlying mechanisms and long-term effects of this botanical treatment.
Quercetin Ameliorated Diabetic Stress through Upregulation of Antioxidant Enzymes and the Nrf-2/HO-1 Axis in the Spleen of Mice
Authors: Sarma Subhrata , Kashyap Anubhuti , Chakrabarti Arjita , Singh Shiv Shankar
Published: January 2025

Diabetes is the enduring endocrine complications increasing with tremendous challenges all over the world. Quercetin, being the predominant flavonoids has pentahydroxy polyphenolic structure that bears antioxidant activity. Our study assessed the efficacy of quercetin in mice against diabetes caused oxidative stress by modifying the radical quencher, the body’s antioxidants and their regulator-Nrf-2 and HO-1. Hyperglycemia attributes significantly (p<0.01) raised levels of lipid peroxidation, lowered levels of catalase, GSH, SOD, higher intracellular ROS generation and suppressed Nrf-2/HO-1 reactivity in studied organ (spleen). Quercetin minimizes the sugar level significantly(p<0.01) in blood, causes restoration of altered spleen and body weight, also bring down the lipid peroxide level significantly (p<0.01) and further enhanced SOD, catalase, GSH antioxidant levels. Both splenic & peritoneal macrophages revealed lower intracellular ROS generation. Quercetin uplifted the reactivity of Nrf-2 & HO-1. Study’s outcome showed that quercetin supplementation attenuated diabetes-caused oxidative stress through upregulation of antioxidant enzymes activity and Nrf-2 and its regulated gene-HO-1 in immune organ-spleen. Thus, quercetin might be fruitful in reducing the ROS-mediated damage in diabetic individuals.
Salutary effect of Commelina benghalensis (Linn.) aerial parts on streptozotocin induced diabetes linked complications in rat: Effective extract selection study
Authors: Das Shibani , Pal Dibya , Singharoy Sajani , Lohar Pampa , Hazra Sukriti , Ghosh Debidas
Published: January 2025

The experiment was designed to identify the most effective extract of Commelina benghalensis aerial parts for the rectification of carbohydrate metabolic disorder, oxidative stress and hyperlipidemia in streptozotocin-induced diabetic rats, developed with a single intramuscular injection of streptozotocin (4 mg/0.1 M citrate buffer/0.1 kg body weight). Diabetic rats were treated with 0.02 g of hydro-methanol (60:40) or hydro-ethanol (60:40) or aqueous extract orally per 0.1 kg body weight in 0.5 ml distilled water for 28 days and sacrificed on 29th day. Glycemic sensors levels, carbohydrate metabolizing enzyme activities, lipid profile, oxidative stress, toxicity markers, and pancreatic histological changes were assessed using standard protocols. Treatment with mentioned extracts of Commelina benghalensis significantly (p<0.05) improved body weight, fasting blood glucose, serum insulin, lipid profile, and carbohydrate metabolic enzyme kinetics in the liver, kidney and skeletal muscle in treated groups compared to untreated diabetic control group. Additionally, treatment of said extracts of Commelina benghalensis significantly restored (p<0.05) the levels of oxidative stress sensors and pancreatic histology towards untreated control group. Among the extracts, hydro-ethanolic extract showed highest efficacy for rectifying glycemic status, lipid profile, and oxidative stress condition for developing a safe, affordable and multi-targeted anti-diabetic herbal drug.
Implementation of Asymmetrical Decentralization In Papua: Relevance to Demands and Problems
Authors: Angela Deni , Nursadi Harsanto
Published: January 2025

This research aims to analyze the implementation of asymmetric decentralization in Indonesia with the case study of Papua. Efforts to end the conflict in Papua are a priority for the Indonesian government. This research uses qualitative research methods packaged in a normative legal approach, including historical and legal approaches as primary sources for research. Based on the special autonomy law, there’s potential and equitable development through articles that provide opportunities for Indigenous Law 2/21. Because several changes lead to equality and the elimination of inequality. However, in practice, asymmetric decentralization can possibly become a problem, especially regarding the threat of conflict due to expansion and acts of corruption, collusion, and nepotism. In practice, several indicators have not been able to provide a solution to the separatism movement in Papua, especially regarding the peace agreement. The Implementation of special autonomy has not been a complete solution. Meanwhile, in the relevance of special autonomy, Law 2/21 sporadically has been by the requests represented DPRD included in the expense of discrimination and development allocation. However, in several demands, the Indonesian government has not been able to answer one of which is dialogue and openness of journalistic activities.
PENGUJIAN HIPOTESIS PASAR EFISIEN BENTUK LEMAH DAN PERAMALAN INDEKS HARGA SAHAM GABUNGAN MENGGUNAKAN BOX JENKINS MODEL
Authors: Setiawan Abdurrahman , Mahadewi Lufina
Published: January 2025

Penelitian ini bertujuan untuk menguji hipotesis pasar efisien bentuk lemah pada indeks harga saham gabungan periode September 2019 sampai Mei 2023 dan meramalkan indeks harga saham gabungan untuk 15 hari kedepan dengan model yang terbaik. Variabel dalam penelitian menggunakan Indeks Harga Saham Gabungan periode September 2019 sampai Mei 2023.  Metode pengujian  yang digunakan ada dua, yang pertama metode peramalan menggunakan box jenkins model. Proses peramalan dimulai dari identifikasi model (tingkat stasioneritas data, menentukan nilai AR dan MA), estimasi parameter dari model yang terpilih, diagnostic checking, lalu forecasting menggunakan model yang terbaik. Untuk menguji hipotesis pasar efisien menggunakan uji autokorelasi atau durbin watson test.  Terkait pengujian hipotesis pasar efisien bentuk lemah terbukti ada di indeks harga saham gabungan dan metode peramalan yang cocok adalah ARIMA (3,1,15) dengan tingkat akurasi 95.23% serta hasil peramalan tidak jauh berbeda dengan data aktual 15 hari kedepan.  Implikasi manajerial dari penelitian ini adalah harus berhati-hati dalam proses pengambilan keputusan investasi dan pendanaan di pasar efisien bentuk lemah, karena informasi publik sudah tercermin dalam harga saham. Selain itu, perlu menerapkan strategi manajemen risiko untuk melindungi nilai dari fluktuasi pasar.
PENGARUH CELEBRITY ENDORSEMENT TERHADAP PURCHASE BEHAVIOUR PRODUK SKINCARE
Authors: Apriliani Dian Rahayu , Pakpahan Arnolt Kristian
Published: January 2025

Penelitian ini bertujuan untuk menguji pengaruh trustworthiness, expertise, attractiveness, congruence terhadap credibility dan menguji pengaruh credibility terhadap purchase intention, premium price. Penelitian ini menguji riset secara simultan. Alat analisis yang digunakan untuk menguji hipotesis ini adalah Multivariat Structural Equation Model [SEM]. Hasil pengujian ini menunjukan terdapat pengaruh positif trustworthiness, expertise, attractiveness, congruence terhadap credibility dan pengaruh credibility terhadap purchase intention, premium price. Implikasi manajerial dari penelitian ini adalah lebih meningkatkan keyakinan konsumen dengan informasi/rekomendasi yang diberikan oleh selebriti mengenai skincare dengan cara mengidentifikasi dan mencari selebriti yang memiliki hubungan baik dengan para pengikutnya dan dapat menginformasikan nilai - nilai dari brand secara efektif dan efisien, membuat selebriti yang akan merekomendasikan produk skincare, memiliki kesan selebriti ahli di bidang tersebut dengan cara melakukan pembekalan materi untuk selebriti tersebut agar lebih memahami bidang dan spesifik produk yang akan dia tawarkan kepada para konsumen.
Persepsi Masyarakat terhadap Keberadaan Peternak Ayam Broiler di Kelurahan Rijang Pitu Kecamatan Maritengngae Kabupaten Sidenreng Rappang
Authors: Iksan M , Nugraha A , Armayani A
Published: January 2025

The aim of this research is to find out how the residents of Rijang Pitu sub-district, Maritengngae District, and Sidenreng Rappang Regency perceive the existence of broiler chickens. The research was conducted from July 29 to September 29 2023 at Rijang Pitu Village, Maritengngae District, Sidenreng Rappang Regency. sample used in this research were people who lived close to broiler chicken breeders with a total of 38 respondents. Samples were taken using purposive sampling. The type of study carried out in this research is descriptive and quantitative with a Likert scale. To measure the subvariables odor, fly population, and livestock waste, the measurement scale is very disturbed, disturbed, quite disturbed, not disturbed, and very not disturbed. The results of the research show that the odor generated from livestock is in the moderately disturbed category with a weight value of 374, the fly population generated from livestock is in the disturbed category with a weight value of 392, and the waste generated from livestock is in the moderately disturbed category with a weight value of 366. It can be concluded that the perception of society as a whole is in the moderately disturbed category with an overall weight of 1.132.
Kajian Kesejahteraan Hewan dan Hubungannya dengan Karakteristik Peternak pada Peternakan Kambing di Manokwari
Authors: Zurahmah N , Masriani M
Published: January 2025

This study aims to assess the level of welfare of goats kept intensively in Manokwari, and to analyze the relationship with characteristics of traditional farmers and the level of welfare of the goats they keep. The research was conducted in an area densely populated with goats (Prafi and Masni districts), in Manokwari. The research sample of 21 goat farms, selected by purposive sampling, was used in this study. Animal welfare assessment was carried out using the Animal Needs Index (ANI) method which was modified according to goats. The results showed that only 33% of goat farms in the study area were classified as prosperous. There is a significant relationship (P<0.05) between age and rearing scale with the welfare level of the goats they keep. Therefore, it is necessary to pay attention to the age of the breeder and the scale of rearing goats in Manokwari in an effort to improve the welfare of the goats kept.
Psikologi Pembisnis dan Kesejahteraan Subyektif; Pergolakan Manajemen Strategis UMKM Produk Peternakan dan Pertanian di Jawa Timur Dalam menghadapi Dinamika Bisnis
Authors: Suryawan A , Hartono B , Putritamara J , Syamsulhadi M
Published: January 2025

The role of psychology in subjective well-being among entrepreneurs has been well-established by previous researchers, but this study focuses on small businesses in the potential products of honey and coffee commodities. Therefore, this research delves deeper into the influence of entrepreneurs' psychology on subjective well-being in small and medium enterprises (SMEs) specializing in honey and coffee in East Java. This study utilized cross- sectional data, surveying 175 integrated entrepreneurs in honeybee and coffee farming in East Java using a questionnaire instrument. The data analysis employed a quantitative approach with Partial Least Square-Structural Equation Modeling (PLS-SEM).The empirical study proves that entrepreneurs' psychology, specifically optimism, positively affects subjective well-being. Farmers with higher levels of optimism can drive entrepreneurs towards achieving subjective well-being, including life satisfaction, good household conditions, and happiness. Therefore, to achieve subjective well-being, entrepreneurs should maintain positive expectations when facing business uncertainties, always believe in generating ample revenue, envision a promising future, hope for things to go as planned, believe in miracles, and maintain optimism towards better fortunes beyond expectations. This study has implications for businesses of all sizes, emphasizing the measurement of intangible resources in achieving subjective well-being
TAXONOMY OF THE KATYDIDS (ORTHOPTERA: TETTIGONIIDAE) FROM EAST ASIA AND ADJACENT ISLANDS. COMMUNICATION 17
Authors:   Zoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, St. Petersburg, 199034, Russia, Gorochov A. V.
Published: January 2025

New material on the genera Elbenia Stål and Phaulula Bol. (Phaneropterinae:
Holochlorini) is reviewed. The species composition of the latter genus
is discussed. Eight new taxa from Indonesia, Malaysia and the Philippines are
described: Sulapteron subgen. n. in the genus Elbenia s. l.; E. (S.) kendariensis sp.
n.; E. (Tamdaopteron) tembelingi sp. n.; Ph. javanica sp. n.; Ph. spinulenta sp. n.;
Ph. tapienomima sp. n.; Ph. tuberculata sp. n.; Ph. figurata sp. n.
NEW FOR THE FAUNA OF BELARUS FAMILY DIAPRIIDAE (HYMENOPTERA: DIAPRIOIDEA)
Authors:   Department of Public Health and Health with the course of the Faculty of Professional Development and Retraining, Gomel State Medical University, Lange Str. 5, 246000 Gomel, Belarus, Ostrovsky A. M.
Published: January 2025

The family Diapriidae and genus Belyta Jurine, 1807 are newly recorded from
Belarus. based on Belyta depressa Thomson, 1858. Brief diagnostic characters, illustrations
and information on geographical distribution of B. depressa Thomson, 1858 are provided.
E2F1-mediated ESPL1 transcriptional activation predicts poor prognosis and promotes the proliferation of leiomyosarcoma
Authors: Yang Xiaojuan , Miao Guihua , Wang Qin , Yu Qin , Hu Qinsheng , Tan Gang
Published: January 2025

Objective:

Soft tissue and bone cancers, collectively known as sarcomas, constitute a diverse array of uncommon tumors originating from connective tissues. Among sarcomas, leiomyosarcoma (LMS) is one of the most frequently encountered subtypes. This study aims to investigate the expression, clinical significance, biological regulation, and dysregulation mechanisms of extra spindle pole bodies like 1 (ESPL1), a gene critical for cell cycle regulation in LMS.

Material and Methods:

Bioinformatics analysis was performed using the data from The Cancer Genome Atlas-Sarcoma and Genotype-Tissue Expression datasets. Functional experiments to assess cell proliferation and the cell cycle were performed in LMS cells (SK-LMS-1) after ESPL1 knockdown. Bioinformatics analyses were conducted to identify the potential transcriptional regulators of ESPL1. The regulatory relationship between ESPL1 and the E2F transcription factor 1 (E2F1) was validated through the various molecular assays.

Results:

ESPL1 is significantly overexpressed in LMS compared with normal muscle tissue. High ESPL1 expression is associated with a shorter progression-free interval (PFI) in sarcoma patients, particularly in the LMS subset. ESPL1 expression might be an independent prognostic factor for poor overall survival and PFI in LMS patients. Functional studies in the LMS cell line SK-LMS-1 demonstrated that ESPL1 knockdown slowed cell proliferation and increased G2/M cell cycle arrest, suggesting its crucial role in maintaining LMS cell viability and genomic integrity. Further bioinformatics analysis identified the E2F1 transcription factor as a key regulator of ESPL1 expression in LMS. Mechanistic investigations demonstrated that E2F1 interacts with the ESPL1 promoter, leading to transcriptional activation.

Conclusion:

These findings highlight the ESPL1-E2F1 axis as a potential prognostic biomarker and therapeutic target in LMS.
Presentation specificity of gluttonic communication in Japanese culinary anime
Authors: Rufova E. S. , Matannanova A. A.
Published: January 2025

This article is devoted to the problem of the gluttonic picture of the world and its implementation in the form of Japanese gluttonic discourse as a phenomenon with high communicative significance. The relevance of the study is due to the fact that detailing the ethnocultural specificity of the sign reflection of the gluttonic aspect of the ligvosemiotic picture of the world helps to improve intercultural understanding and strengthen ties between different communities. The study is also of interest due to the small number of studies in this area and the growing interest in the study of gluttonic discourse, which is one of the most important types of communication. The purpose of the study is to identify the presentation specificity of Japanese gluttonic communication using culinary anime as an example. As a result of the study, we come to the conclusion that the presentation function of anime is realized through gluttony signs as a means of influencing society and, therefore, forms dominant cultural preferences and gastronomic stereotypes of behavior. In anime, there is a clear consistency of non-verbal means with verbal components that allow us to interpret the image. The presentation of anime is realized through adjectives and adverbs that convey a clearly expressed positive assessment when describing the taste of a dish, its texture and other qualities, which may include descriptions of dishes, the general atmosphere, relationships with other participants in the discourse. Color, which builds an associative series, additional graphic elements and sound accompaniment, acquires special significance in the presentation of gluttony signs in anime. Moreover, the setting of the frame, the use of a close-up, as well as quickly changing pictures become an additional means of artistic impact on the viewer, enhancing the effect of gluttony communication.
Linguistic means of expressing irony as an aesthetic category of the comic in the idiostyle of M.E. Saltykov-Shchedrin
Authors: Sultanova E. A.
Published: January 2025

Irony, acting as a heterogeneous phenomenon in language, has attracted the attention of many researchers and philosophers since Antiquity. In the course of this study we identified its main features and established that irony is an artistic form of evaluative depiction of reality, the distinctive feature of which is a hidden ridicule opposed to the literal meaning of what is said. This feature of irony is an important tool for revealing many social and philosophical problems in the works of writers and poets of all times, which explains its integral role in the development of fiction literature. M. E. Saltykov-Shchedrin is one of the most prominent representatives of the satirical-ironic direction in Russian literature of the XIX century. This article is devoted to the analysis and systematisation of lexical and syntactic means of creating irony in the works of M. E. Saltykov-Shchedrin through the study of the writer’s early and late works and the identification of his ideological position towards the social situation of Russia at that time. On the basis of the method of philological analysis of the text and the method of comparison there is a detailed description of each identified means of expression of ironic modality in the writer’s satirical tales, as well as in his novel “The History of One City”. The example of his works shows irony in the form of sarcastic and sharp mockery, which indicates the democratic nature of the writer’s ideas and his hatred of autocracy. This work has an important linguistic and general cultural nature. The results and materials of the research can be used in linguographic practice, in the process of teaching courses on linguistic and cultural studies, Russian as a foreign (non-native) language in Russian and foreign universities.