bluuebunny/crossref_metadata_2025 huggingface dataset:
Validation of Polymorphisms Associated with the Risk of Radiation-Induced Oesophagitis in an Independent Cohort of Non-Small-Cell Lung Cancer Patients
Authors: Aguado-Barrera Miguel E. , Martínez-Calvo Laura , Fernández-Tajes Juan , Calvo-Crespo Patricia , Taboada-Valladares Begoña , Lobato-Busto Ramón , Gómez-Caamaño Antonio , Vega Ana
Published: March 2021

Several studies have identified single-nucleotide polymorphisms (SNPs) associated with adverse effects in non-small-cell lung cancer (NSCLC) patients treated with radiation therapy. Here, using an independent cohort, we aimed to validate the reported associations. We selected 23 SNPs in 17 genes previously associated with radiation-induced oesophagitis for validation in a cohort of 178 Spanish NSCLC patients. Of them, 18 SNPs were finally analysed, following the methods described in the original published studies. Two SNPs replicated their association with radiation-induced oesophagitis (rs7165790 located in the BLM gene: odds ratio (OR) = 0.16, 95% CI = 0.04–0.65, p-value = 0.010; rs4772468 at FGF14: OR = 4.36, 95% CI = 1.15–16.46, p-value = 0.029). The SNP rs2868371 at HSPB1 was also validated but displayed an opposite effect to the formerly described (OR = 3.72; 95% CI = 1.49–9.25; p-value = 0.004). Additionally, we tested a meta-analytic approach including our results and the previous datasets reported in the referenced publications. Twelve SNPs (including the two previously validated) retained their statistically significant association with radiation-induced oesophagitis. This study strengthens the role of inflammation and DNA double-strand break repair pathways in the risk prediction of developing radiation-induced oesophagitis in NSCLC patients. The validated variants are good candidates to be evaluated in risk prediction models for patient stratification based on their radiation susceptibility.
Circulating Cell-Free DNA in Liquid Biopsies as Potential Biomarker for Bladder Cancer: A Systematic Review
Authors: Herranz Raquel , Oto Julia , Plana Emma , Fernández-Pardo Álvaro , Cana Fernando , Martínez-Sarmiento Manuel , Vera-Donoso César D. , España Francisco , Medina Pilar
Published: March 2021

Bladder cancer (BC) is among the most frequent cancer types in the world and is the most lethal urological malignancy. Presently, diagnostic and follow-up methods for BC are expensive and invasive. Thus, the identification of novel predictive biomarkers for diagnosis, progression, and prognosis of BC is of paramount importance. To date, several studies have evidenced that cell-free DNA (cfDNA) found in liquid biopsies such as blood and urine may play a role in the particular scenario of urologic tumors, and its analysis may improve BC diagnosis report about cancer progression or even evaluate the effectiveness of a specific treatment or anticipate whether a treatment would be useful for a specific patient depending on the tumor characteristics. In the present review, we have summarized the up-to-date studies evaluating the value of cfDNA as potential diagnostic, prognostic, or monitoring biomarker for BC in several biofluids.
Comparative Effectiveness of Techniques in Targeted Prostate Biopsy
Authors: Sugano Dordaneh , Kaneko Masatomo , Yip Wesley , Lebastchi Amir H. , Cacciamani Giovanni E. , Abreu Andre Luis
Published: March 2021

In this review, we evaluated literature regarding different modalities for multiparametric magnetic resonance imaging (mpMRI) and mpMRI-targeted biopsy (TB) for the detection of prostate cancer (PCa). We identified studies evaluating systematic biopsy (SB) and TB in the same patient, thereby allowing each patient to serve as their own control. Although the evidence supports the accuracy of TB, there is still a proportion of clinically significant PCa (csPCa) that is detected only in SB, indicating the importance of maintaining SB in the diagnostic pathway, albeit with additional cost and morbidity. There is a growing subset of data which supports the role of TB alone, which may allow for increased efficiency and decreased complications. We also compared the literature on transrectal (TR) vs. transperineal (TP) TB. Although further high-level evidence is necessary, current evidence supports similar csPCa detection rate for both approaches. We also evaluated various TB techniques such as cognitive fusion biopsy (COG-TB) and in-bore biopsy (IB-TB). COG-TB has comparable detection rates to software fusion, but is operator-dependent and may have reduced accuracy for smaller lesions. IB-TB may allow for greater precision as lesions are directly targeted; however, this is costly and time-consuming, and does not account for MRI-invisible lesions.
Monocyte Subsets and Serum Inflammatory and Bone-Associated Markers in Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma
Authors: Damasceno Daniela , Almeida Julia , Teodosio Cristina , Sanoja-Flores Luzalba , Mayado Andrea , Pérez-Pons Alba , Puig Noemi , Arana Paula , Paiva Bruno , Solano Fernando , Romero Alfonso , Matarraz Sergio , van den Bossche Wouter B. L. , Flores-Montero Juan , Durie Brian , van Dongen Jacques J. M. , Orfao Alberto
Published: March 2021

Background. Monocyte/macrophages have been shown to be altered in monoclonal gammopathy of undetermined significance (MGUS), smoldering (SMM) and active multiple myeloma (MM), with an impact on the disruption of the homeostasis of the normal bone marrow (BM) microenvironment. Methods: We investigated the distribution of different subsets of monocytes (Mo) in blood and BM of newly-diagnosed untreated MGUS (n = 23), SMM (n = 14) and MM (n = 99) patients vs. healthy donors (HD; n = 107), in parallel to a large panel of cytokines and bone-associated serum biomarkers. Results: Our results showed normal production of monocyte precursors and classical Mo (cMo) in MGUS, while decreased in SMM and MM (p ≤ 0.02), in association with lower blood counts of recently-produced CD62L+ cMo in SMM (p = 0.004) and of all subsets of (CD62L+, CD62L− and FcεRI+) cMo in MM (p ≤ 0.02). In contrast, intermediate and end-stage non-classical Mo were increased in BM of MGUS (p ≤ 0.03), SMM (p ≤ 0.03) and MM (p ≤ 0.002), while normal (MGUS and SMM) or decreased (MM; p = 0.01) in blood. In parallel, increased serum levels of interleukin (IL)1β were observed in MGUS (p = 0.007) and SMM (p = 0.01), higher concentrations of serum IL8 were found in SMM (p = 0.01) and MM (p = 0.002), and higher serum IL6 (p = 0.002), RANKL (p = 0.01) and bone alkaline phosphatase (BALP) levels (p = 0.01) with decreased counts of FcεRI+ cMo, were restricted to MM presenting with osteolytic lesions. This translated into three distinct immune/bone profiles: (1) normal (typical of HD and most MGUS cases); (2) senescent-like (increased IL1β and/or IL8, found in a minority of MGUS, most SMM and few MM cases with no bone lesions); and (3) pro-inflammatory-high serum IL6, RANKL and BALP with significantly (p = 0.01) decreased blood counts of immunomodulatory FcεRI+ cMo-, typical of MM presenting with bone lesions. Conclusions: These results provide new insight into the pathogenesis of plasma cell neoplasms and the potential role of FcεRI+ cMo in normal bone homeostasis.
Optical Management of CQD/AgNP@SiNW Arrays with Highly Efficient Capability of Dye Degradation
Authors: Hsiao Po-Hsuan , Timjan Sasimontra , Kuo Kuan-Yi , Juan Joon-Ching , Chen Chia-Yun
Published: March 2021

The facile synthetic method for the preparation of incorporated carbon quantum dots (CQDs)/Ag nanoparticles (AgNPs) with well-aligned silicon nanowire (SiNW) arrays is demonstrated, offering the superior photodegradation capabilities covering UV to visible wavelength regions. By examining the morphology, microstructure, crystallinity, chemical feature, surface groups, light-emitting, and reflection characteristics, these hybrid heterostructures are systematically identified. Moreover, the involving degradation kinetics, band diagram, cycling capability, and underlying mechanism of photodegradation are investigated, validating their remarkable and reliable photocatalytic performances contributed from the strongly reduced light reflectivity, superior capability of charge separation, and sound wettability with dye solutions.
Microglial Pruning: Relevance for Synaptic Dysfunction in Multiple Sclerosis and Related Experimental Models
Authors: Geloso Maria Concetta , D’Ambrosi Nadia
Published: March 2021

Microglia, besides being able to react rapidly to a wide range of environmental changes, are also involved in shaping neuronal wiring. Indeed, they actively participate in the modulation of neuronal function by regulating the elimination (or “pruning”) of weaker synapses in both physiologic and pathologic processes. Mounting evidence supports their crucial role in early synaptic loss, which is emerging as a hallmark of several neurodegenerative diseases, including multiple sclerosis (MS) and its preclinical models. MS is an inflammatory, immune-mediated pathology of the white matter in which demyelinating lesions may cause secondary neuronal death. Nevertheless, primitive grey matter (GM) damage is emerging as an important contributor to patients’ long-term disability, since it has been associated with early and progressive cognitive decline (CD), which seriously worsens the quality of life of MS patients. Widespread synapse loss even in the absence of demyelination, axon degeneration and neuronal death has been demonstrated in different GM structures, thus raising the possibility that synaptic dysfunction could be an early and possibly independent event in the neurodegenerative process associated with MS. This review provides an overview of microglial-dependent synapse elimination in the neuroinflammatory process that underlies MS and its experimental models.
Endometriosis Is Associated with Functional Polymorphism in the Promoter of Heme Oxygenase 1 (HMOX1) Gene
Authors: Milewski Łukasz , Ścieżyńska Aneta , Ponińska Joanna , Soszyńska Marta , Barcz Ewa , Roszkowski Piotr I. , Kamiński Paweł , Włodarski Paweł , Płoski Rafał , Malejczyk Jacek
Published: March 2021

Endometriosis is a common gynecological disorder characterized by the ectopic growth of endometrial-like tissue outside the uterine cavity. Etiopathogenesis of endometriosis is poorly understood; it is plausible, however, that the disease may be associated with oxidative stress related to local heme and iron metabolism. Therefore, the aim of the study was to reveal a possible association of endometriosis with a stress-inducible heme oxygenase 1 (HMOX1). For this purpose, 228 patients with clinically confirmed endometriosis and 415 control parous women from general Polish population were examined for functional –413A>T (rs2071746) single-nucleotide polymorphism (SNP) and (GT)n dinucleotide repeat length polymorphism in the promoter of HMOX1 gene. In addition, –413A>T SNP was assessed by the specific TaqMan® SNP Genotyping Assay, and (GT)n polymorphism was determined by PCR product size analysis. We found that endometriosis is associated with an increased frequency of −413A(GT)31,32 haplotype (OR (95%CI) = 1.27 (1.01–1.60), p = 0.0381) and −413A(GT)31,32 homozygous genotype [OR (95%CI) = 1.51 (1.06–2.17), p = 0.0238]. These data suggest that endometriosis is associated with functional polymorphism of HMOX1 gene, and this gene may play a part in the pathogenesis of this disorder.
Fenamates as Potential Therapeutics for Neurodegenerative Disorders
Authors: Hill Jaunetta , Zawia Nasser H.
Published: March 2021

Neurodegenerative disorders are desperately lacking treatment options. It is imperative that drug repurposing be considered in the fight against neurodegenerative diseases. Fenamates have been studied for efficacy in treating several neurodegenerative diseases. The purpose of this review is to comprehensively present the past and current research on fenamates in the context of neurodegenerative diseases with a special emphasis on tolfenamic acid and Alzheimer’s disease. Furthermore, this review discusses the major molecular pathways modulated by fenamates.
Oxidative Stress Conditions Result in Trapping of PHF-Core Tau (297–391) Intermediates
Authors: Maina Mahmoud B. , Al-Hilaly Youssra K. , Burra Gunasekhar , Rickard Janet E. , Harrington Charles R. , Wischik Claude M. , Serpell Louise C.
Published: March 2021

The self-assembly of tau into paired helical filaments (PHFs) in neurofibrillary tangles (NFTs) is a significant event in Alzheimer’s disease (AD) pathogenesis. Numerous post-translational modifications enhance or inhibit tau assembly into NFTs. Oxidative stress, which accompanies AD, induces multiple post-translational modifications in proteins, including the formation of dityrosine (DiY) cross-links. Previous studies have revealed that metal-catalysed oxidation (MCO) using Cu2+ and H2O2 leads to the formation of DiY cross-links in two misfolding proteins, Aβ and α-synuclein, associated with AD and Parkinson’s disease respectively. The effect of MCO on tau remains unknown. Here, we examined the effect of MCO and ultra-violet oxidation to study the influence of DiY cross-linking on the self-assembly of the PHF-core tau fragment. We report that DiY cross-linking facilitates tau assembly into tau oligomers that fail to bind thioflavin S, lack β-sheet structure and prevents their elongation into filaments. At a higher concentration, Cu2+ (without H2O2) also facilitates the formation of these tau oligomers. The DiY cross-linked tau oligomers do not cause cell death. Our findings suggest that DiY cross-linking of pre-assembled tau promotes the formation of soluble tau oligomers that show no acute impact on cell viability.
Investigation into Microstructure, Wear Resistance in Air and NaCl Solution of AlCrCoNiFeCTax High-Entropy Alloy Coatings Fabricated by Laser Cladding
Authors: Zhao Peng , Li Jun , Lei Ruyan , Yuan Baige , Xia Manman , Li Xiao , Zhang Ying
Published: March 2021

AlCrCoNiFeCTax (x = 0, 0.5 and 1.0) high-entropy alloys coatings were synthesized on 45# steel by laser cladding. The microstructural evolution of the coatings with the change in x was analyzed in detail. The effect of Ta content on the wear behaviors of the coatings at different circumstances (in air and 3.5 wt.% NaCl solution) was especially highlighted. The microstructure presented the following change: equiaxed BCC (Body Centered Cubic) grains + fine MC (carbide, M = Al, Cr, Co and Ni) particles (x = 0) → equiaxed BCC grains + coarse TaC blocks + fine TaC particles (x = 0.5) → flower-like BCC grains + coarse TaC blocks + eutecticum (BCC + TaC) (x = 1.0). The average microhardness of the coatings demonstrated an upward tendency with increasing x due to the combination of the stronger solid solution and dispersion strengthening from the significant difference in atomic radius between Ta and Fe and the formation of TaC with an extremely high hardness. The wear rates of the coatings were gradually reduced both in air and in NaCl solution along with the increase in Ta content, which were lower than those of the substrate. The wear rates of the coatings with x = 0.5 and 1.0 in NaCl solution were slightly reduced by about 17% and 12% when compared with those in air. However, the values of the substrate and the coating without Ta in NaCl solution were sharply enhanced by 191% and 123% when compared with those in air. This indicated that the introduction of Ta contributed to the improvement in wear resistance both in air and in NaCl solution.
Effect of Additional Order in Two-Stage Supply Chain Contract under the Demand Uncertainty
Authors: Chueanun Suphannee , Suwandechochai Rawee
Published: March 2021

In this work, mathematical models are formulated in order to investigate the effect of the additional order on the expected total profit of a two-stage supply chain. A multi-period buyback contract between a supplier and a retailer under the demand uncertainty is considered. Under the contract, an advance order is submitted to the supplier in advance when the demand is unknown, and an additional order can be made at the beginning of each period after the previous period demand is realized. The impact of the coordination on the supply chain’s expected total profit is also considered. The results show that the additional order does not always increases the supply chain profit. The additional order increases the supply chain profit only when both the retailer and supplier are coordinated. Under the decentralized system with the buyback contract, the retailer tends to order less in an advance order to reduce the risk. This leads to the higher cost due the additional order after the demand is realized. As a result, it is lowers the supply chain profit. Moreover, the sensitivity analysis is performed using numerical studies in order to observe the behavior of the expected total profit of the supply chain.
Emotion Transfer for 3D Hand and Full Body Motion Using StarGAN
Authors: Chan Jacky C. P. , Ho Edmond S. L.
Published: March 2021

In this paper, we propose a new data-driven framework for 3D hand and full-body motion emotion transfer. Specifically, we formulate the motion synthesis task as an image-to-image translation problem. By presenting a motion sequence as an image representation, the emotion can be transferred by our framework using StarGAN. To evaluate our proposed method’s effectiveness, we first conducted a user study to validate the perceived emotion from the captured and synthesized hand motions. We further evaluate the synthesized hand and full body motions qualitatively and quantitatively. Experimental results show that our synthesized motions are comparable to the captured motions and those created by an existing method in terms of naturalness and visual quality.
Numerical Simulation of Microstructure Evolution in Solidification Process of Ferritic Stainless Steel with Cellular Automaton
Authors: Wang Wenli , Shi Qin , Zhu Xu , Liu Yinhua
Published: March 2021

In order to study the basic principles of vibration-excited liquid metal nucleation technology, a coupled model to connect the temperature field calculated by ANSYS Fluent and the dendritic growth simulated by cellular automaton (CA) algorithm was proposed. A two-dimensional CA model for dendrite growth controlled by solute diffusion and local curvature effects with random zigzag capture rule was developed. The proposed model was applied to simulate the temporal evolution of solidification microstructures under different degrees of surface undercooling and vibration frequency of the crystal nucleus generator conditions. The simulation results showed that the predicted columnar dendrites regions were more developed, the ratio of interior equiaxed dendrite reduced and the size of dendrites increased with the increase of the surface undercooling degrees on the crystal nucleus generator. It was caused by a large temperature gradient formed in the melt. The columnar-to-equiaxed transition (CET) was promoted, and the refined grains and homogenized microstructure were also achieved at the high vibration frequency of the crystal nucleus generator. The influences of the different process parameters on the temperature gradient and cooling rates in the mushy zone were investigated in detail. A lower cooling intensity and a uniform temperature gradient distribution could promote nucleation and refine grains. The present research has guiding significance for the process parameter selection in the actual experimental.
Simultaneous Multi-Bragg Peak Coherent X-ray Diffraction Imaging
Authors: Lauraux Florian , Labat Stéphane , Yehya Sarah , Richard Marie-Ingrid , Leake Steven J. , Zhou Tao , Micha Jean-Sébastien , Robach Odile , Kovalenko Oleg , Rabkin Eugen , Schülli Tobias U. , Thomas Olivier , Cornelius Thomas W.
Published: March 2021

The simultaneous measurement of two Bragg reflections by Bragg coherent X-ray diffraction is demonstrated on a twinned Au crystal, which was prepared by the solid-state dewetting of a 30 nm thin gold film on a sapphire substrate. The crystal was oriented on a goniometer so that two lattice planes fulfill the Bragg condition at the same time. The Au 111 and Au 200 Bragg peaks were measured simultaneously by scanning the energy of the incident X-ray beam and recording the diffraction patterns with two two-dimensional detectors. While the former Bragg reflection is not sensitive to the twin boundary, which is oriented parallel to the crystal–substrate interface, the latter reflection is only sensitive to one part of the crystal. The volume ratio between the two parts of the twinned crystal is about 1:9, which is also confirmed by Laue microdiffraction of the same crystal. The parallel measurement of multiple Bragg reflections is essential for future in situ and operando studies, which are so far limited to either a single Bragg reflection or several in series, to facilitate the precise monitoring of both the strain field and defects during the application of external stimuli.
Clinicopathological Significances of Tumor–Stroma Ratio (TSR) in Colorectal Cancers: Prognostic Implication of TSR Compared to Hypoxia-Inducible Factor-1α Expression and Microvessel Density
Authors: Kang Guhyun , Pyo Jung-Soo , Kim Nae-Yu , Kang Dong-Wook
Published: March 2021

The present study aimed to elucidate the clinicopathological significance and prognostic implications of tumor–stroma ratio (TSR) in colorectal cancers (CRCs). TSRs were investigated in 266 human CRC specimens. The correlations between TSR and clinicopathological characteristics and survival were evaluated. The hypoxia-inducible factor-1α (HIF-1α) immunohistochemical expression of tumor cells and microvessel density (MVD) of stroma were compared between stroma-low and stroma-high subgroups. Results: Stroma-low was found in 185 of 266 CRCs (69.5%). Stroma-low was significantly correlated with less frequent vascular and perineural invasion and distant metastasis than stroma-high. HIF-1α of tumor cells was more highly expressed in the stroma-high subgroup than in the stroma-low subgroup. In addition, MVD was significantly higher in the stroma-high subgroup compared to the stroma-low subgroup. The stroma-low rate was increased considerably in CRCs with a mucinous component and decreased in CRCs with a micropapillary component. There were significant correlations between stroma-low and better overall and recurrence-free survivals. Similar to the literature, we observed that stroma-low was significantly correlated with favorable tumor behaviors and better survival. The microscopic examination of TSR can be useful for predicting the prognosis of CRC patients.
Characteristic Features of Infrared Thermographic Imaging in Primary Raynaud’s Phenomenon
Authors: Lindberg Lotte , Kristensen Bent , Thomsen Jane F. , Eldrup Ebbe , Jensen Lars T.
Published: March 2021

Raynaud’s phenomenon (RP) is characterized by the episodic whitening of the fingers upon exposure to cold. Verification of the condition is crucial in vibration-exposed patients. The current verification method is outdated, but thermographic imaging seems promising as a diagnostic replacement. By investigating patients diagnosed with RP, the study aimed at developing a simple thermographic procedure that could be applied to future patients where verification of the diagnosis is needed. Twenty-two patients with primary RP and 58 healthy controls were examined using thermographic imaging after local cooling of the hands for 1 min in water of 10°C. A logistic regression model was fitted with the temperature curve characteristics to convey a predicted probability of having RP. The characteristics time to end temperature and baseline temperature were the most appropriate predictors of RP among those examined (p = 0.004 and p = 0.04, respectively). The area under the curve was 0.91. The cut-off level 0.46 yielded a sensitivity and specificity of 82% and 86%, respectively. The positive and negative predictive values were 69% and 93%, respectively. This newly developed thermographic method was able to distinguish between patients with RP and healthy controls and was easy to operate. Thus, the method showed great promise as a method for verification of RP in future patients. Trial registration: ClinicalTrials.gov NCT03094910.
Comparing Metabolomics Profiles in Various Types of Liquid Biopsies among Screening Participants with and without Advanced Colorectal Neoplasms
Authors: Erben Vanessa , Poschet Gernot , Schrotz-King Petra , Brenner Hermann
Published: March 2021

Analysis of metabolomics has been suggested as a promising approach for early detection of colorectal cancer and advanced adenomas. We investigated and compared the metabolomics profile in blood, stool, and urine samples of screening colonoscopy participants and aimed to evaluate differences in metabolite concentrations between people with advanced colorectal neoplasms and those without neoplasms. Various types of bio-samples (plasma, feces, and urine) from 400 participants of screening colonoscopy were investigated using the MxP® Quant 500 kit (Biocrates, Innsbruck, Austria). We detected a broad range of metabolites in blood, stool, and urine samples (504, 331, and 131, respectively). Significant correlations were found between concentrations in blood and stool, blood and urine, and stool and urine for 93, 154, and 102 metabolites, of which 68 (73%), 126 (82%), and 39 (38%) were positive correlations. We found significant differences between participants with and without advanced colorectal neoplasms for concentrations of 123, 49, and 28 metabolites in blood, stool and urine samples, respectively. We detected mostly positive correlations between metabolite concentrations in blood samples and urine or stool samples, and mostly negative correlations between urine and stool samples. Differences between subjects with and without advanced colorectal neoplasms were found for metabolite concentrations in each of the three bio-fluids.
Identification of Brain Damage after Seizures Using an MR-Based Electrical Conductivity Imaging Method
Authors: Kim Sanga , Choi Bup Kyung , Park Ji Ae , Kim Hyung Joong , Oh Tong In , Kang Won Sub , Kim Jong Woo , Park Hae Jeong
Published: March 2021

Previous imaging studies have shown the morphological malformation and the alterations of ionic mobility, water contents, electrical properties, or metabolites in seizure brains. Magnetic resonance electrical properties tomography (MREPT) is a recently developed technique for the measurement of electrical tissue properties with a high frequency that provides cellular information regardless of the cell membrane. In this study, we examined the possibility of MREPT as an applicable technique to detect seizure-induced functional changes in the brain of rats. Ultra-high field (9.4 T) magnetic resonance imaging (MRI) was performed, 2 h, 2 days, and 1 week after the injection of N-methyl-D-aspartate (NMDA; 75 mg/kg). The conductivity images were reconstructed from B1 phase images using a magnetic resonance conductivity imaging (MRCI) toolbox. The high-frequency conductivity was significantly decreased in the hippocampus among various brain regions of NMDA-treated rats. Nissl staining showed shrunken cell bodies and condensed cytoplasm potently at 2 h after NMDA treatment, and neuronal cell loss at all time points in the hippocampus. These results suggest that the reduced electrical conductivity may be associated with seizure-induced neuronal loss in the hippocampus. Magnetic resonance (MR)-based electrical conductivity imaging may be an applicable technique to non-invasively identify brain damage after a seizure.
The Role of the Family Network When Raising a Child with a Disability in Low- and Middle-Income Countries
Authors: Bizzego Andrea , Lim Mengyu , Dimitriou Dagmara , Esposito Gianluca
Published: March 2021

Family plays a role in supporting child development, by facilitating caregiving and other parental practices. Low- and middle-income families typically have a complex structure with many relatives living together in the same household. The role of family and family complexity in the caregiving of children with disabilities is still unknown. In this study, we use data from N = 22,405 children with severe (N = 876) and mild or no disability (N = 21,529) from a large dataset collected in the 2005–2007 Multiple Indicator Cluster Survey. In particular, we adopt PageRank, a well-known algorithm used by search engines, to quantify the importance of each child in the family network. We then analyze the level of caregiving the child received in light of the child’s importance and developmental status, using a generalized linear model. Results show a main effect of child’s importance and of the interaction of child’s importance and developmental status. Post hoc analysis reveals that higher child importance is associated with a better caregiving outcome only for children with mild or no disability.
The Quantum Regularization of Singular Black-Hole Solutions in Covariant Quantum Gravity
Authors: Tessarotto Massimo , Cremaschini Claudio
Published: March 2021

An excruciating issue that arises in mathematical, theoretical and astro-physics concerns the possibility of regularizing classical singular black hole solutions of general relativity by means of quantum theory. The problem is posed here in the context of a manifestly covariant approach to quantum gravity. Provided a non-vanishing quantum cosmological constant is present, here it is proved how a regular background space-time metric tensor can be obtained starting from a singular one. This is obtained by constructing suitable scale-transformed and conformal solutions for the metric tensor in which the conformal scale form factor is determined uniquely by the quantum Hamilton equations underlying the quantum gravitational field dynamics.
Impact of the Reduction of Diesel Fuel Subsidy in the Design of an Off-Grid Hybrid Power System: A Case Study of the Bellavista Community in Ecuador
Authors: Hidalgo-Leon Ruben , Amoroso Fernando , Litardo Jaqueline , Urquizo Javier , Torres Miguel , Singh Pritpal , Soriano Guillermo
Published: March 2021

This paper presents a technical, economic, and environmental analysis and optimization of the impact of the reduction of diesel fuel subsidy in the design of an off-grid hybrid power system (OHPS). The OHPS includes a diesel generator, battery energy storage system (BESS), and a solar power system (SPS). This impact will focus on the electricity production levels of each of the OHPS components according to the increase of the fuel price and the SPS size. The Bellavista community in Ecuador was selected as the case study for this work. In this South American country, the government has begun a gradual increase in the diesel fuel price until it reaches international prices. Fifteen scenarios of OHPSs were simulated, in Homer Pro software, considering three SPS sizes and varying the diesel fuel price in five values. The annual load profile for the simulations was built based on the information of a previous study in this community. The results showed that for lower fuel prices (USD$0.26/L and USD$0.35/L), the OHPSs worked mostly with their diesel generators with reduced use of their BESSs. However, there was a higher penetration of the power delivered from the SPSs and BESSs, with higher fuel prices (USD$0.44/L, USD$0.53/L, and USD$0.62/L). These OHPSs considerably reduced their CO2 emissions compared with the standalone diesel generator scenario.
Role of Local Investments in Creating Rural Development in Poland
Authors: Kozera Agnieszka , Dworakowska-Raj Małgorzata , Standar Aldona
Published: March 2021

Poland’s rural areas are characterised by a significant degree of development diversity, especially in the case of communes—the smallest territorial units. For development to take place, the entity responsible for its creation must incur the necessary investment expenditures. By implementing the public tasks assigned to them, communes are responsible for ensuring sustainable local development, which indirectly affects regional development and shapes the development of the entire country. Therefore, this article aims to assess the level and diversity of rural commune investment activities and their impact on the development of Poland’s rural areas. The empirical research conducted was aimed at verifying the following hypothesis: “The highest investment activity can be observed primarily in rural communes located in Eastern Poland”. The Regions of Eastern Poland are characterised by their peripheral location, both in the country and all of the EU. The research covered the 2007–2018 period due to a particular increase in local investment projects thanks to such factors as obtaining EU funding, as well as others. The research included one- and multidimensional analyses of the phenomenon examined with the use of the TOPSIS method to obtain the value of the synthetic measure of the rural commune investment activity level. The designated investment activity classes were the basis for the analysis of their links with their location within the macroregion, as well as their socio-economic status. The research has shown that the most pro-investment municipalities are located primarily in the Southern and Eastern Macroregions while those spending the least on investment projects are situated in the North-Western and Northern Macroregions. Thus, the research hypothesis put forth in the article has been confirmed. This delimitation is due to additional state resources being provided to the less developed eastern areas of Poland. Citizens living in these regions can not only take advantage of the funding provided as part of the Regional Operational Programmes, but also the Eastern Poland Programme dedicated to helping them.
Application of Regression and ANN Models for Heat Pumps with Field Measurements
Authors: Puttige Anjan Rao , Andersson Staffan , Östin Ronny , Olofsson Thomas
Published: March 2021

Developing accurate models is necessary to optimize the operation of heating systems. A large number of field measurements from monitored heat pumps have made it possible to evaluate different heat pump models and improve their accuracy. This study used measured data from a heating system consisting of three heat pumps to compare five regression and two artificial neural network (ANN) models. The models’ performance was compared to determine which model was suitable during the design and operation stage by calibrating them using data provided by the manufacturer and the measured data. A method to refine the ANN model was also presented. The results indicate that simple regression models are more suitable when only manufacturers’ data are available, while ANN models are more suited to utilize a large amount of measured data. The method to refine the ANN model is effective at increasing the accuracy of the model. The refined models have a relative root mean square error (RMSE) of less than 5%.
A Procedure to Calculate First-Order Wave-Structure Interaction Loads in Wave Farms and Other Multi-Body Structures Subjected to Inhomogeneous Waves
Authors: Rodrigues José Miguel
Published: March 2021

A typical assumption when performing analytical, numerical, and experimental studies in wave–structure interaction in multi-body problems such as for wave farms and very large floating structures is the homogeneity of the wave field. Important interactions between the floating elements are dependent on the direction, amplitude, and phase of the waves acting on each. Then, wave homogeneity is probably unrealistic in near-shore areas where these installations are to be deployed. In the present work, an existing interaction method, which allows the use of standard boundary element diffraction codes for solving the first order wave structure linear potential for each unique geometry in the problem, is shown to be able to account for inhomogeneous sea states across the domain of a multi-body problem requiring only minimal modification to its implementation. A procedure to use the method to include arbitrary incoming undisturbed wave conditions at each body is presented. A verification study was done by using an artificial numerical configuration to mimic an inhomogeneous wave field in a standard diffraction code, which was used as a reference. The results obtained using the interaction-method based procedure are shown to be in excellent agreement with the reference ones. Furthermore, an example of frequency inhomogeneity of the wave field in a wave farm is shown and the effects on the motion amplitudes and absorbed power are presented illustrating the applicability of the procedure.
Is the Seasonal Variation in Frost Resistance and Plant Performance in Four Oak Species Affected by Changing Temperatures?
Authors: Preißer Maggie , Bucher Solveig Franziska
Published: March 2021

Research Highlights: We found seasonal variation in frost resistance (FR) and plant performance which were affected by growth temperature. This helps to better understand ecophysiological processes in the light of climate change. Background and Objectives: FR and photosynthesis are important plant characteristics that vary with the season. The aim of this study was to find out whether there is a seasonal variation in FR, photosynthetic CO2 assimilation rates and leaf functional traits associated with performance such as specific leaf area (SLA), leaf dry matter content (LDMC), chlorophyll content, stomatal characteristics and leaf thickness in two evergreen and two deciduous species, and whether this is influenced by different temperature treatments. Additionally, the trade-off between FR and photosynthetic performance, and the influence of leaf functional traits was analyzed. By understanding these processes better, predicting species behavior concerning plant performance and its changes under varying climate regimes can be improved. Materials and Methods: 40 individuals of four oak species were measured weekly over the course of ten months with one half of the trees exposed to frost in winter and the other half protected in the green house. Two of these species were evergreen (Quercus ilex L., Quercus rhysophylla Weath.), and two were deciduous (Quercus palustris L., Quercus rubra L.). We measured FR, the maximum assimilation rate at light saturation under ambient CO2 concentrations (Amax), chlorophyll fluorescence and the leaf functional traits SLA, LDMC, stomatal pore area index (SPI), chlorophyll content (Chl) and leaf thickness. Results: All parameters showed a significant species-specific seasonal variation. There was a difference in all traits investigated between evergreen and deciduous species and between the two temperature treatments. Individuals that were protected from frost in winter showed higher photosynthesis values as well as SLA and Chl, whereas individuals exposed to frost had overall higher FR, LDMC, SPI and leaf thickness. A trade-off between FR and SLA, rather than FR and photosynthetic performance was found.
A Web Interface for Analyzing Hate Speech
Authors: Vrysis Lazaros , Vryzas Nikolaos , Kotsakis Rigas , Saridou Theodora , Matsiola Maria , Veglis Andreas , Arcila-Calderón Carlos , Dimoulas Charalampos
Published: March 2021

Social media services make it possible for an increasing number of people to express their opinion publicly. In this context, large amounts of hateful comments are published daily. The PHARM project aims at monitoring and modeling hate speech against refugees and migrants in Greece, Italy, and Spain. In this direction, a web interface for the creation and the query of a multi-source database containing hate speech-related content is implemented and evaluated. The selected sources include Twitter, YouTube, and Facebook comments and posts, as well as comments and articles from a selected list of websites. The interface allows users to search in the existing database, scrape social media using keywords, annotate records through a dedicated platform and contribute new content to the database. Furthermore, the functionality for hate speech detection and sentiment analysis of texts is provided, making use of novel methods and machine learning models. The interface can be accessed online with a graphical user interface compatible with modern internet browsers. For the evaluation of the interface, a multifactor questionnaire was formulated, targeting to record the users’ opinions about the web interface and the corresponding functionality.
Vehicular Communication Management Framework: A Flexible Hybrid Connectivity Platform for CCAM Services
Authors: Naudts Dries , Maglogiannis Vasilis , Hadiwardoyo Seilendria , van den Akker Daniel , Vanneste Simon , Mercelis Siegfried , Hellinckx Peter , Lannoo Bart , Marquez-Barja Johann , Moerman Ingrid
Published: March 2021

In the upcoming decade and beyond, the Cooperative, Connected and Automated Mobility (CCAM) initiative will play a huge role in increasing road safety, traffic efficiency and comfort of driving in Europe. While several individual vehicular wireless communication technologies exist, there is still a lack of real flexible and modular platforms that can support the need for hybrid communication. In this paper, we propose a novel vehicular communication management framework (CAMINO), which incorporates flexible support for both short-range direct and long-range cellular technologies and offers built-in Cooperative Intelligent Transport Systems’ (C-ITS) services for experimental validation in real-life settings. Moreover, integration with vehicle and infrastructure sensors/actuators and external services is enabled using a Distributed Uniform Streaming (DUST) framework. The framework is implemented and evaluated in the Smart Highway test site for two targeted use cases, proofing the functional operation in realistic environments. The flexibility and the modular architecture of the hybrid CAMINO framework offers valuable research potential in the field of vehicular communications and CCAM services and can enable cross-technology vehicular connectivity.
Anguilliform Locomotion across a Natural Range of Swimming Speeds
Authors: Tack Nils B. , Du Clos Kevin T. , Gemmell Brad J.
Published: March 2021

Eel-like fish can exhibit efficient swimming with comparatively low metabolic cost by utilizing sub-ambient pressure areas in the trough of body waves to generate thrust, effectively pulling themselves through the surrounding water. While this is understood at the fish’s preferred swimming speed, little is known about the mechanism over a full range of natural swimming speeds. We compared the swimming kinematics, hydrodynamics, and metabolic activity of juvenile coral catfish (Plotosus lineatus) across relative swimming speeds spanning two orders of magnitude from 0.2 to 2.0 body lengths (BL) per second. We used experimentally derived velocity fields to compute pressure fields and components of thrust along the body. At low speeds, thrust was primarily generated through positive pressure pushing forces. In contrast, increasing swimming speeds caused a shift in the recruitment of push and pull propulsive forces whereby sub-ambient pressure gradients contributed up to 87% of the total thrust produced during one tail-beat cycle past 0.5 BL s−1. This shift in thrust production corresponded to a sharp decline in the overall cost of transport and suggests that pull-dominated thrust in anguilliform swimmers is subject to a minimum threshold below which drag-based mechanisms are less effective.
Integration of Fluidic Nozzles in the New Low Emission Dual Fuel Combustion System for MGT Gas Turbines
Authors: Ćosić Bernhard , Waßmer Dominik , Genin Franklin
Published: March 2021

Fluidic oscillators have proven their capabilities and advantages in terms of the generation of oscillating jets without moving parts for many years, mainly in experimental studies. In this paper, the design, development, and integration of fluidic atomizers into the liquid-fuel system of the dual-fuel low NOX Advanced Can Combustion (ACC) system of the MAN Gas Turbines (MGT) are presented. The two-stage system comprises a pressure-swirl nozzle as a pilot stage and an assembly of four main premixed nozzles, based on fluidic technology. The design and the features of the pilot nozzle are briefly presented, whereas the focus lies on the functionality and layout of the fluidic nozzles. The complete integration, validation, and verification of this innovative liquid-fuel injection unit are presented. The final system features fast fuel-switchovers, low complexity, high reliability, and dry low emissions in liquid-fuel operation.
Optimization of High Hydrostatic Pressure Treatments on Soybean Protein Isolate to Improve Its Functionality and Evaluation of Its Application in Yogurt
Authors: Wang Chenxiao , Yin Hao , Zhao Yanyun , Zheng Yan , Xu Xuebing , Yue Jin
Published: March 2021

This work aimed to improve the functional properties of soybean protein isolate (SPI) by high hydrostatic pressure (HHP) and develop SPI incorporated yogurt. Response surface methodology (RSM) was used to optimize the HHP treatment parameters, including pressure, holding time, and the ratio of SPI/water. Water holding capacity, emulsifying activity index, solubility, and hardness of SPI gels were evaluated as response variables. The optimized HPP treatment conditions were 281 MPa of pressure, 18.92 min of holding time, and 1:8.33 of SPI/water ratio. Water and oil holding capacity, emulsifying activity, and stability of SPI at different pH were improved. Additionally, relative lipoxygenase (LOX) activity of HHP treated SPI (HHP-SPI) was decreased 67.55 ± 5.73%, but sulphydryl group content of HHP-SPI was increased 12.77%, respectively. When incorporating 8% of SPI and HHP-SPI into yogurt, the water holding capacity and rheological properties of yogurt were improved in comparison with yogurt made of milk powders. Moreover, HHP-SPI incorporated yogurt appeared better color and flavor.
Genome Analysis of Endotrypanum and Porcisia spp., Closest Phylogenetic Relatives of Leishmania, Highlights the Role of Amastins in Shaping Pathogenicity
Authors: Albanaz Amanda T. S. , Gerasimov Evgeny S. , Shaw Jeffrey J. , Sádlová Jovana , Lukeš Julius , Volf Petr , Opperdoes Fred R. , Kostygov Alexei Y. , Butenko Anzhelika , Yurchenko Vyacheslav
Published: March 2021

While numerous genomes of Leishmania spp. have been sequenced and analyzed, an understanding of the evolutionary history of these organisms remains limited due to the unavailability of the sequence data for their closest known relatives, Endotrypanum and Porcisia spp., infecting sloths and porcupines. We have sequenced and analyzed genomes of three members of this clade in order to fill this gap. Their comparative analyses revealed only minute differences from Leishmaniamajor genome in terms of metabolic capacities. We also documented that the number of genes under positive selection on the Endotrypanum/Porcisia branch is rather small, with the flagellum-related group of genes being over-represented. Most significantly, the analysis of gene family evolution revealed a substantially reduced repertoire of surface proteins, such as amastins and biopterin transporters BT1 in the Endotrypanum/Porcisia species when compared to amastigote-dwelling Leishmania. This reduction was especially pronounced for δ-amastins, a subfamily of cell surface proteins crucial in the propagation of Leishmania amastigotes inside vertebrate macrophages and, apparently, dispensable for Endotrypanum/Porcisia, which do not infect such cells.