bluuebunny/crossref_metadata_2025 huggingface dataset:
Critical incidents associated with pediatric anesthesia: changes over 6 years at a tertiary children’s hospital
Authors: Cho Sung-Ae , Lee Ji-Hyun , Ji Sang-Hwan , Jang Young-Eun , Kim Eun-Hee , Kim Hee-Soo , Kim Jin-Tae
Published: September 2022

Background: Sustained interest is needed in the characteristics of critical incidents in pediatric anesthesia and related changes, for determining the causes and degree of potential harm; this will also improve the quality of medical care. This study aimed to analyze the incidence of critical incidents recorded in 2014–2019, and to compare them with those in 2008–2013.Methods: Critical incidents associated with pediatric anesthesia, including cardiac arrest, recorded in a voluntary departmental reporting system between January 2014 and December 2019 were compared with those reported between January 2008 and August 2013 using chi-square test.Results: We identified 295 (0.55%) critical incidents from 53,541 cases of pediatric anesthesia (3,471 cardiothoracic surgeries); this is consistent with the previously reported incidence of 0.46%. Among the critical incidents, the incidences of adverse events, sentinel event, near miss case and no-harm events were 93.9%, 1.7%, 0%, and 6.1% in 2014–2019, whereas those were 98.3%, 2.6%, 1.7%, and 0% in 2008–2013 (P = 0.023, 0.686, 0.080, and < 0.001, respectively). Cardiac arrest accounted for 25 (8.5%) cases of the 295 critical events, which significantly lower than that previously reported (18.3%; P = 0.020). Human factor-related events accounted for 61.0% of all critical incidences; this was similar to the previous data (58.5%).Conclusions: Over six years, there has been no significant difference in the total incidence of critical events. Despite the decrease in the incidence of serious critical events, perioperative care in pediatric anesthesia can be further improved.
Design, Synthesis, In Silico ADMET Studies and Anticancer Activity of Some New Pyrazoline and Benzodioxole Derivatives
Authors: Tok Fatih , Erdoğan Ömer , Çevik Özge , Koçyiğit-Kaymakçıoğlu Bedia
Published: September 2022

A new series of 2-pyrazoline derivatives starting from substituted benzodioxole chalcones were designed and synthesized. IR and 1H NMR spectral data and elemental analysis were used to characterize the structures of the synthesized compounds. The cytotoxic activities on HeLa, MCF-7 cancer cell lines and NIH-3T3 for these compounds were tested by using MTT assay. Among the synthesized compounds 2d, 2j, 3j and 3n against MCF-7 cells, and 3c against HeLa exhibited significant cytotoxic activity with IC50 between 10.08 and 27.63 μM. Compound 3f showed the most potent anticancer activity against both cancer cells with good selectivity (IC50 = 11.53 μM on HeLa with SI = 81.75 and IC50 = 11.37 μM on MCF-7 with SI = 82.90). Furthermore, in silico ADMET analyses were performed and the drug-likeness properties of the compounds were investigated.
Synthesis, Biological Evaluation, and Molecular Docking Studies of Hydrazones as Novel Xanthine Oxidase Inhibitors
Authors: Xue Ling-Wei , Li Shi-Tong , Han Yong-Jun , Luo Xiao-Qiang
Published: September 2022

A series of hydrazones, 2-cyano-N’-(4-diethylamino-2-hydroxybenzylidene)acetohydrazide (1), N’-(5-bromo-2-hydroxy-3-methoxybenzylidene)-3-chlorobenzohydrazide monohydrate (2·H2O), N’-(2-hydroxy-3-methylbenzylidene)-4-nitrobenzohydrazide (3), and N’-(2-hydroxy-3-trifluoromethoxybenzylidene)-4-nitrobenzohydrazide (4), were prepared and structurally characterized by elemental analysis, IR and 1H NMR spectra, and single crystal X-ray determination. Xanthine oxidase inhibitory activities of the compounds were studied. Among the compounds, 2-cyano-N’-(4-diethylamino-2-hydroxybenzylidene)acetohydrazide shows the most effective activity. Docking simulation was performed to insert the compounds into the crystal structure of xanthine oxidase at the active site to investigate the probable binding modes.
Nested VP1 PCR and Nanopore Sequencing from Stool and ES Samples v1.2 v3
Authors: Shaw Alex , Majumdar Manasi , Troman Catherine , OToole Aine , not provided c.ansley , not provided rachel.colquhoun , not provided khurshida , not provided alammu , Rambaut Andrew , Martin Javier , Grassly Nick , ARSHAD YASIR , Bujaki Erika , Akello Joyce
Published: September 2022

This protocol is updated from the protocol described in the paper "Rapid and sensitive direct detection and identification of poliovirus from stool and environmental surveillance samples using nanopore sequencing" by Shaw et al in the Journal of Clinical Microbiology (2020), DOI: 10.1128/JCM.00920-20 and is commonly known as Direct Detection of Poliovirus by Nanopore Sequencing (DDNS). The protocol aims to amplify the VP1 region of poliovirus through a nested PCR using panEV primers followed by amplification of the VP1 sequence using the Q8/Y7 primer set. We advise the use of barcoded primers where possible as this greatly simplifies the subsequent library preparation process. Additional steps have however been included in the case that the second PCR step is performed either with standard Q8/Y7 primers or using Q8/Y7 with the barcode adaptors (BCA) attached. Primer sequences for the panEV primers, Q8/Y7 primers and modified Q8/Y7 primers are found in Dataset S1 of the publication. Sequencing of the panEV product is also possible through the removal of the VP1 nested PCR steps and the preparation of the panEV product for sequencing. Version updates: v1.1 – Updated to LSK-109 v1.2 – Minor formatting edits v1.3 – Streamlining and minor alterations v1.4 – Written inclusion of negative control in PCR v1.5 – Updated to dreamtaq and Y7R primers v1.6 – Updated MinKNOW instructions & minor edits v1.7 – Changed back to Y7 primers v1.10 – Updated to LSK-110, removed cleaning and quantification prior to pooling, updated taq volume in rtPCR, altered nested VP1 to use neat panEV product. v1.11 – Updated RNA extraction to include Proteinase K, removed DNA CS addition, added pooling of Y7/Q8 primers. v1.2 - Updated authorship, addition of nOPV2 primers
Laboratory Protocols v1
Authors: Saptawati Leli
Published: September 2022

Nontuberculous mycobacterial (NTM) were identified using matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS), and tests were performed to determine antibiotic susceptibility, the ability to form biofilms, sliding motility characteristics, and the ability to adhere to and invade pneumocyte cells.
Phylogenetic Analysis of Complete Bovine Coronavirus Genome Sequences v3
Authors: M Workman Aspen
Published: September 2022

Bovine coronavirus (BCoV) has spilled over to many species, including humans, where the host range variant coronavirus OC43 is endemic. Balance of the opposing activities of the surface spike (S) and hemagglutinin esterase (HE) glycoproteins control virion avidity which is critical for interspecies transmission and host adaptation. Here, 78 genomes were sequenced directly from clinical samples collected between 2013 and 2022 from cattle in 12 states, primarily in the Midwestern U.S. Relatively little genetic diversity was observed, with genomes having >98% nucleotide identity. Eleven isolates collected between 2020 and 2022 from four states (Nebraska, Colorado, California, and Wisconsin) contained a 12-nucleotide insertion in the receptor-binding domain (RBD) of the HE gene identical to one recently reported in China, and a single genome from Nebraska collected in 2020 contained a novel 12-nucleotide deletion in the HE gene RBD. Isogenic HE proteins containing either the insertion or deletion in the HE RBD maintained esterase activity and the ability to bind bovine submaxillary mucin, a substrate enriched in the receptor 9-O-acetylated-sialic acid, despite modeling that predicted structural changes in the HE R3 loop critical for receptor binding. The emergence of BCoV with structural variants in the RBD raises the possibility of further interspecies transmission.
ADE 2022 Day 2: DNA Extraction v2
Authors: Little Tom
Published: September 2022

DAY 2: ADE field practical instructions for DNA extraction
Use of a metaplasticity-based protocol of therapeutic transcranial magnetic stimulation in patients with progressive multiple sclerosis and spasticity: first experience
Authors: Bakulin I. S. , Poydasheva A. G. , Zabirova A. H. , Lagoda D. Yu. , Rimkevichus A. A. , Zakharova M. N. , Suponeva N. A. , Piradov M. A.
Published: September 2022

Background. Spasticity is a disabling syndrome frequently observed in progressive multiple sclerosis. One of the promising approaches to the treatment of spasticity is the use of therapeutic intermittent theta‑burst transcranial magnetic stimulation. In the last time new metaplasticity‑based protocols are being developed in order to increase the effectiveness of this technique. These protocols consist of several stimulation sessions in a day with an interval between sessions. However, there is no experience of use of such protocols in spasticity so far.Aim. To assess the safety and tolerability as well as provide first evidence of anti‑spastic effects of an original meta-plasticity‑based intermittent theta‑burst stimulation protocol in patients with progressive multiple sclerosis and spasticity.Materials and methods. In total, 5 patients with progressive multiple sclerosis and spasticity (2 females and 3 males, 28–53 y. o., disease duration – 11–18 years, EDSS – 6.5–8.5 points) were included into the study. 3 sessions of stimulation separated by an interval of 1 hour were applied daily, where a single session consisted of 3 protocols of theta‑burst stimulation with standard duration. Stimulation target was the area of cortical representation of the leg muscles, stimulation was applied consequently to both sides during 5 days (15 sessions in total). Before and after the treatment course anti‑spastic effect (modified Ashworth scale) as well as spasticity‑related pain, fatigue and clinical global impression were assessed.Results. No serious adverse events were observed during the study. Mild adverse events (sleepiness, pain at the stimulation site) developed in some cases, which did not affect patients’ willing to continue participation in the study. After the stimulation course decrease in spasticity in the legs was registered in 4 of 5 patients (to 12–39 % from the basic level). Decrease of fatigue (4 / 5) and pain severity (3 / 5) was also observed.Conclusion. According to the first experience, the proposed original metaplasticity‑based transcranial magnetic stimulation protocol is safe, well‑tolerable and potentially effective in patients with progressive multiple sclerosis. Therefore the further investigation of the protocol in a randomized controlled study seems justified.
The first family case of spinocerebellar ataxia type 14 in Russia
Authors: Nuzhnyy E. P. , Abramycheva N. Yu. , Klyushnikov S. A. , Illarioshkin S. N.
Published: September 2022

Spinocerebellar ataxia type 14 (SCA14) is a rare neurodegenerative disease with a predominant cerebellar affection and autosomal dominant inheritance. A characteristic clinical presentation is slowly progressive cerebellar ataxia, hyperreflexia, cognitive impairment and movement disorders (dystonia and myoclonus). Clinical and genetic characteristics of the first familial case of SCA14 in Russia (a 77‑year‑old female patient) caused by heterozygous pathogenic mutation c.155G>C (p.Cys52Ser) in exon 1 in PRKCG gene (NM_002739.1) are presented. The total duration of the disease was 47 years, and the follow‑up period was 32 years. The disease phenotype corresponded to isolated ataxia with a slow rate of progression; brain MRI revealed atrophy of the cerebellar vermis and hemispheres, symmetrical hyperintensity of the dentate nucleus on T2‑weighted images. The features of the SCA14 clinical presentation and the effect of mutations in the regulatory and kinase domains of protein kinase C gamma on the formation of pure and complex phenotypes are discussed.
Abdominal pocketing for the child’s fingers for hand replantation: A clinical case
Authors: Bogdanov Sergey B. , Aristov Andrey M. , Kurinnyy Sergey N. , Nikishev Vladislav A. , Mukhanov Mikhail L. , Vedeneeva Anastasia L.
Published: September 2022

BACKGROUND: The traumatic avulsion of fingers is a severe injury that requires emergency replantation. In the case of crush mechanism and degloving, direct vessel repair, and reconstruction may be impossible. It appears fair to use indirect revascularization by enveloping the phalanges after osteosynthesis with blood-supplied tissues. The most accessible anatomically and functionally are the lower abdominal flap and abdominal pocketing of the hand; the flap pedicle was cut and the hand was released. Staged surgeries could be required for functional and aesthetic reconstruction of acceptable soft tissue envelop of the hand.
CLINICAL CASE: This is a clinical case of successful treatment of traumatic avulsion of II, III, IV fingers on the right hand by phalanx osteosynthesis and abdominal pocketing. The restoration of the functionally and aesthetically acceptable finger was achieved.
DISCUSSION: There is a high risk of necrosis and stump formation after the replantation of severely injured fingers when there was any possibility for direct vessel repair. Another method is the indirect revascularization by blood vessel ingrowth from the flap through the fracture line. This saves the injured limb segment.
CONCLUSIONS: The presented technique of abdominal pocketing allows the replantation of the avulsed and degloved fingers without microsurgical vascular operation and restores them. It appears fair to recommend this technique as a salvage surgery in the case of severely injured soft tissues of the avulsed hand fingers.
GTUNE: An Assembled Global Seismic Dataset of Underground Nuclear Test Blasts
Authors: Barama Louisa , Peng Zhigang , Newman Andrew V. , Williams Jesse
Published: September 2022

From catalogs of available declassified underground nuclear explosions, we compiled a comprehensive seismic waveform and event catalog termed GTUNE (Georgia Tech Underground Nuclear Explosions). Nuclear blast seismic records are sourced from previously prepared published datasets and openly available waveforms from online sources. All seismic traces were assembled into a user-friendly format compatible with most python-based machine learning (ML) packages. The GTUNE dataset includes the raw seismogram time series, event coordinates and origin time, sampling rate, station metadata, channel, epicentral distance, and P-wave arrival time from the origin dataset when available and otherwise identified using a tuned automated picker. This is the first openly available comprehensive global underground nuclear blast seismic dataset and consists of 28,123 vertical-component waveforms from 774 nuclear test blasts between 1961 and 2017 recorded between 0 and 90 epicentral degrees. For stations where data are not directly included due to data-sharing restrictions, the mechanisms to acquire and process these data are included. In this article, we describe various steps involved in data collection and quality control to ensure accurate labels, and present summary properties of the catalog and data set. The catalog was initially developed for applications with ML methods but can be used for a wide range of studies such as source physics, earth structure, and event detection methodological development.
Evaluation of critical value and urgent sample notification at clinical biochemistry laboratory in a tertiary care hospital
Authors: Menezes Geraldine , Peter Stella S. , Shanthaveeranna Girish K.
Published: September 2022

Background: Critical value and urgent sample result notification is widely accepted in the diagnostic fraternity as an important factor, as it may affect patient care and safety. Timely release and notification of these test result as per the individual laboratory protocol becomes an internal part of quality reporting system. The present study was aimed at evaluating the effective implementation of the existing protocol of urgent clinical notification (UCN) in the clinical biochemistry laboratory of tertiary care hospital and evaluating the turnaround time for urgent samples and critical results listed under UCN protocol in the clinical biochemistry laboratory.Methods: A prospective, observational study was conducted in clinical biochemistry laboratory of a tertiary care hospital. Descriptive statistics was calculated for all the data by Mann-Whitney and Wilcoxon test.Results: Majority of the critical results were informed to the clinical personnel by the clinical laboratory.  Out of 4687 critical results, 25.41% critical results were informed directly to the ward through telephonic communication. Documentation of critical values in the dedicated register and lab information system (LIS) was 25.41% and 40.28% respectively. 421 (9%) out of 4687 critical results were not notified. The median turnaround time for all urgent tests and critical results was found to be 72.33 minutes and 76.00 minutes respectively.Conclusions: This study highlighted various approaches to improve the critical value notification and its turnaround time and status of UCN in laboratory
An Overview of Self-Adaptive Differential Evolution Algorithms with Mutation Strategy
Authors: AlKhulaifi Dania , AlQahtani Maryam , AlSadeq Zenab , Rahman Atta-ur , Musleh Dhiaa
Published: September 2022

Differential Evolution (DE) is a widely used global searching algorithm that solves real-world optimization problems. It is categorized as a stochastic parameter optimization method that has a broad spectrum of applications, notably neural networks, logistics, scheduling, and modeling. In practice, different optimization issues need different parameter settings. Due to DE simplicity, ease of implementation, and dependability, many scientists were interested in examining this algorithm. Nonetheless, the quality of DE and its variations are directly influenced by different mutation techniques and control parameter settings. In this paper, an overview and analogy of some algorithms that employ different mutation techniques will be illustrated. Additionally, a novel strategy that uses different mutation methods is proposed and compared with some existing strategies.
Design of Integral Sliding Mode Control for Seismic Effect Regulation on Buildings with Unmatched Disturbance
Authors: Husain Suha S. , MohammadRidha Taghreed
Published: September 2022

In this work the robustness properties of Integral Sliding Mode Controller (ISMC) are studied for the problem structural vibration. Selecting the sliding manifold which takes into account the attenuation of the unmatched perturbation is studied as well. This study has two scenarios the first is comparing two types of sliding mode controllers which designed to control 3-story scaled structure supported by Magneto Rheological Damper (MRD). ISMC is compared to classical Sliding Mode Control (SMC) performance, the two controllers checked under effect of Mexico city earthquake. The second scenario compares the proposed controller with other controllers from literature under effect of time scaled El Centro 1940 earthquake. The results show that ISMC performance is better than SMC in scenario one and better than other controllers in scenario two. All the simulation results are obtained by MATLAB.
Ambiwalentne i problemowe relacje w sieciach krewnych: rekonesans
Authors: Przybył Iwona
Published: September 2022

Artykuł prezentuje wyniki badań empirycznych na temat relacji ambiwalentnych i problemowych w sieciach krewnych kobiet i mężczyzn w trzech fazach życia: wczesnego dorosłego, dorosłego w średnim wieku i młodszego seniora, a także na temat tych ról rodzinnych, które są najbardziej narażone na doświadczanie ambiwalencji uczuć. Badania były prowadzone w 2021 roku na próbie 109 osób w wieku 20-69 lat, które umieszczały swoich żyjących krewnych w diagramach relacji bliskich emocjonalnie oraz problemowych. Relacje zostały wtórnie kategoryzowane jako wyłącznie bliskie, wyłącznie problemowe i ambiwalentne na podstawie pozycjonowania krewnych w diagramach. Uzyskane dane wskazują, że emocje w sieci krewnych różnią się w zależności od: stopnia pokrewieństwa, stopnia bliskości danej jednostki z krewnym/-ną oraz od fazy życia. Najbardziej narażone na ambiwalencję są diady: siostra–siostra, matka–córka, syn–ojciec, brat–brat, oraz relacja: syn–matka i siostra–brat. Ponadto, za problemowe uważane są relacje z ciociami i kuzynami. W ostatniej części opracowania podjęto próbę wskazania przyczyn „produkowania” ambiwalencji w tych węzłach sieci krewniaczej wykorzystując m.in. teorię władzy-statusu Theodore D. Kempera.
Analiza argumentacji. Praktyczne implikacje zastosowania modelu argumentacji Stephena Toulmina do analiz danych tekstowych
Authors: Tomanek Krzysztof
Published: September 2022

The text aims to present the analytical solutions rather rarely used in social sciences. These are: the method useful for the discourse analysis (Stephen Toulmin’s model of argument) and text analysis tool used to support the usage of Toulmin’s concept. Few modifications are made to Toulmin’s model and its application. In particular, both are placed within specified fields, also spevified research problems are suggested for them. Examples of how to use both models are described with benefits and problems associated with analytical practice.
School Readiness of Early Childhood Age 5-6 Years Based on The Nijmeegse Schoolbekwaamheid Test (NST)
Authors: Fajariyah Puja Nor
Published: September 2022

School readiness is the achievement of children’s maturity in aspect of development which include physical and motor development, social emotional development, cognitive development, language and communication development, and motivation and work attitude so that they can continue their education to the elementary school level. This study aimed to know the school readiness of early childhood age 5-6 years based on the Nijmeegse Schoolbekwaamheid Test (NST). This study uses quantitative research using a survey method with a target of number 43 early childhood age 5-6 years from 3 different kindergartens of Malang. The instrument using in this study is the Nijmeegse Schoolbekwaamheid Test (NST) which the result test is divided into 3 stages of school readiness called mature, hesitant, and immature. The findings from this study are that early childhood age 5-6 years in Malang kindergartens have school readiness to continue to elementary school level based in the results of the the Nijmeegse Schoolbekwaamheid Test (NST).
Ternary organic solar cells featuring polythiophene
Authors: Qi Qingchun , Ke Huizhen , Ye Long
Published: September 2022

Benefiting from the creation of new photovoltaic materials and innovations in device architectures, organic photovoltaic (OPV) cells are booming. Nonetheless, their prosperity is also accompanied by challenges, such as tedious synthetic routes, increasing costs and insufficient operational stability under practical stresses. Polythiophene, with a simple chemical structure, high scalability and excellent charge transport ability, is expected to be the most promising candidate among all kinds of polymer donors. Ternary mixing, as a simple and effective method for improving the efficiency and stability of OPVs, has attracted significant attention in recent decades. This review provides an overview of the recent advances in ternary OPVs based on polythiophene and discusses the role of various third components in three types of OPV active layers, where polythiophene serves as either the host material or additive, and also clarifies how the third component plays a role in determining morphology and device performance, and finally proposes future research directions for ternary OPVs featuring polythiophene. In short, this review provides insights into polythiophene-based multicomponent systems and helps readers better understand the relationships between morphology, efficiency and stability.
Apakah pendapatan utama dari perkebunan mempengaruhi deforestasi?: Studi dari tingkat desa di Kalimantan, Indonesia
Authors:   Postgraduate of Economic Science, Faculty of Economy and Bussines, Universitas Indonesia and International University of Japan, Yazid Muhammad
Published: September 2022

The complexity of the drivers of deforestation in Indonesia requires various research on deforestation, especially to support policies related to reducing deforestation. The economy is one aspect that contributes to providing an overview of deforestation. This study investigates the relationship between deforestation and village with dominant income from plantation as main commodity. A panel data analysis using data from 3260 villages in Kalimantan in 2011, 2014, and 2018 was analyzed to determine whether plantation as main income affected deforestation. The dependent variable is deforestation in each village. There are 14 independent variables used, with the main variable is villages with main income from plantation as a dummy variable. The results of random effect model show that villages with plantation as main commodity have a positive impact on deforestation. Ten independent variables show a positive relationship with deforestation. Four independent variables show a negative relationship with deforestation, which include the use of firewood, practice of burning land, non-wood small industries, and logging companies (PBPH Hutan Alam). The recommendation of this research is the need to replanting the degraded and non-productive land for the wood plantation areas, thus the conversion of forest into non-forest area can be avoided.
Manfaat, Pandangan, Dan Alternatif Program Perhutanan Sosial Di Dua Kelompok Rujukan, Kabupaten Pemalang
Authors:   Pusat Riset Masyarakat dan Budaya - Badan Riset dan Inovasi Nasional, Jakarta Selatan, Jakarta, 12710, Indonesia, Rachmawan Dicky , Seda Francisia Saveria Sika Ery ,   Departemen Sosiologi FISIP UI, Universitas Indonesia, Kampus Baru UI Depok, Jawa Barat, 10430, Indonesia, Siburian Robert ,   Pusat Riset Masyarakat dan Budaya - Badan Riset dan Inovasi Nasional, Jakarta Selatan, Jakarta, 12710, Indonesia, Sakti Danang Kuncara ,   Bina Usaha Perhutanan Sosial dan Hutan Adat, Kementerian Lingkungan Hidup dan Kehutanan, Jakarta
Published: September 2022

Social forestry program expected to give space for people in forest management, improve livelihood, and conserve the environment at once to decrease poverty rate on people who live around the forest. This article aims to describe the benefit of community member which is complemented by deepening of view and alternative of social forestry program in Kelompok Tani Hutan Gunung Gajah Lestari (KTH GGL) and Lembaga Masyarakat Desa Hutan Rawa Sakti (LMDH RS). Qualitative approach in case study form is used which interview 49 informants include managements and members that accompanied by focus group discussion as data triangulation which processed by Nvivo application and Microsoft Excel. The result shows social forestry program has viewed giving benefit although it felt not optimal by the members in both groups. However, rough calculation on utilization of forest areas tends possibly to contribute around Rp.1,5-3 million rupiah/planting period. The utilization of forest areas is revival means or additional income where social-economic context is needed to be understood on identify commodity alternative that can be developed in the further time. The rest is the need for natural science approach which can answer the confusion of community to find alternatives commodity which more give revenue.
CFIA-Turbidimetric and Photometric Determination of Vitamin B9 (Folic acid) Using LEDs as a Source of Irradiation and Two Solar Cells as an Energy Transducer
Authors: Al-Awadi Nagam S. Turkey , Al-Awadi Nagam S. Turkey
Published: September 2022

A specific, sensitive and simple method was used for the determination of: vitamin B9 (Folic acid) in pure and pharmaceutical formulations using continuous flow injection analysis. The method is based on formation of ion pair compound between folic acid and ammonium molybdate in an aqueous medium to obtain a gray precipitate complex, using homemade; Ayah-6SX1-ST-2D solar cell CFI Analyzer. Optimum parameters was studied to increase the sensitivity for developed method. The linear range for the calibration graph was 0.01-0.6 mMol.L-1 of vitamin B9 and LOD was 131.994 ng/sample with correlation coefficient ( r ) of 0.9810, RSD% was lower than 0.1%, (n=9) for the determination of vitamin B9 at concentration (0.07and 0.5) mMol.L-1 respectively. The developed method was applied successfully for the determination of vitamin B9 in pharmaceutical tablets. A comparison was made between two methods: developed method and the classical UV spectrophotometric method at ?max=255 nm, by using the standard addition method via the use of paired t-test. It showed that there was no significant difference between the developed method and the classical method for determination vitamin B9 at 95% confidence level.
Multi-objective workflow scheduling based on genetic algorithm in cloud environment
Authors: Qiu Huixian , XIA Xuewen
Published: September 2022

In recent years, cloud computing plays a crucial role in many real applications. Thus, how to solve workflow scheduling problems, i.e., allocating and scheduling different resources, under the cloud computing environment becomes more important. Although some evolutionary algorithms (EAs) can solve workflow scheduling problems with smaller large scale, they show some disadvantages on larger scale workflow applications. In this paper, a multi-objective genetic algorithm (MOGA) is applied to optimize workflow scheduling problems. To enhance the search efficiency, this study proposes an initialization scheduling sequence scheme based on tasks’ data sizes. Relying on the initial scheduling sequence, a proper trade-off between the makespan and the energy consumption, which are two optimization objectives in this study, can be achieved. In addition, the longest common subsequence (LCS) of multiple elite individuals is saved. Based on the LCS, the probability of some favorable gene blocks being destroyed will be reduced when performing the crossover operator and the mutate operator. Hence, the integration of the LCS and the selection process in GA can promote the capability of finding the optimal scheduling sequence. The experimental results show that the GA combined with LCS, named as GALCS in this paper, can find a better Pareto front than the ordinary GA as well as other 3 state-of-the-art algorithms. Furthermore, the effectiveness of the new proposed strategies are also verified by a set of experiments.
Effect of Succession Planning Strategies on the Sustainability of Family Businesses in Nigeria
Authors: Awogbemi Johnson Ayoola , Abubakar Hauwa Lamino , Oluwade Omanyo Dorcas
Published: September 2022

The purpose of this research was to investigate the effect of succession planning strategies on the sustainability of family businesses in Nigeria, using mentoring, training, job rotation, coaching, talent management and internship as proxies of succession planning. The calculated sample size of 390 businesses was taken from a target population of 16,363 small businesses in the database of the Nigeria’s National Bureau of Statistics. To provide for anticipated “no response”, 443 questionnaires were administered, out of which 435 (98.2%) returned valid. The data collected was analyzed using descriptive and inferential statistics. Results of analyzed data from the study indicate that mentoring, training, job rotation, coaching, talent management and internship as succession planning strategies are positively correlated to sustainability of family businesses with statistical significance. This is corroborated by the F-test result with F statistic (202.5) larger than (2.01) critical F value and p-value (0.000) which is smaller than the (0.05) alpha level, implying that a significant joint impact of all the independent variables exists on the dependent variable (sustainability). Although the study is located in Nigeria, the results can be generalized to other countries as evidence from literature suggests that family owned businesses worldwide share similar characteristics irrespective of location.
Nonlinear Dynamics Analysis of Rod Fastened Rotor-Combined Support System
Authors: Li Jiaqi , Li Guoqing , Ren Qingzhao , Feng Yinli , Sun Tao
Published: September 2022

Rod fastened rotor is widely used in gas turbine and aero engine. Normally, rod fastened rotor is supported by sliding bearings, but in engineering, improper bearing clearance would lead to severe vibration of the system. Combined support, i.e., squirrel cage-squeeze film damper, could decrease the stiffness and increase the damping of the system. It is usually used to reduce the vibration of integral rotor. In this paper, the dynamic behaviour of rod fastened rotor-combined support system is explored. Based on the Lagrange equation, the equation of motion of rod fastened rotor-squirrel cage-squeeze film damper system is derived. The bistable behaviour and non-synchronous response are disclosed. Besides, a rod fastened rotor-squirrel cage-squeeze film damper bench is built, the bistable behaviour and non-synchronous response are proven experimentally, and the effect of the pressure of the oil film on the nonlinear dynamic behaviour is experimentally explored.
Aβ25-35-induced autophagy and apoptosis are inhibited by the CRMP2-derived peptide ST2-104 (R9-CBD3) via a CaMKK β /AMPK/mTOR signaling hub
Authors: Ji Yingshi , Ren Jinghong , Qian Yuan , Liu Huanyu , Li Jiaxin , Yao Yuan , Khanna Rajesh , Sun Li
Published: September 2022

We previously reported that the peptide ST2-104 (CBD3, for Ca2+ channel-binding domain 3), derived from the collapsin response mediator protein 2 (CRMP2) – a cytosolic phosphoprotein, protects neuroblastoma cells against β-amyloid (Aβ) peptide-mediated toxicity through engagement of a phosphorylated CRMP2/NMDAR pathway. Abnormal aggregation of Aβ peptides (e.g., Aβ 25-35 ) leads to programmed cell death (apoptosis) as well autophagy – both of which contribute to Alzheimer’s disease (AD) progression. Here, we asked if ST2-104 affects apoptosis and autophagy in SH-SY5Y neuroblastoma challenged with the toxic Aβ 25-35 peptide and subsequently mapped the downstream signaling pathways involved. ST2-104 protected SH-SY5Y cells from death following Aβ 25-35 peptide challenge by reducing apoptosis and autophagy as well as limiting excessive calcium entry. Cytotoxicity of SHY-SY5Y cells challenged with Aβ 25-35 peptide was blunted by ST2-104. The autophagy activator Rapamycin blunted the anti-apoptotic activity of ST2-104. ST2-104 reversed Aβ 25-35 -induced apoptosis via inhibiting Ca 2+ /CaM-dependent protein kinase kinase β (CaMKKβ)-mediated autophagy, which was partly enhanced by STO-609 (an inhibitor of CaMKKβ). ST2-104 attenuated neuronal apoptosis by inhibiting autophagy through a CaMKKβ/AMPK/mTOR signaling hub. These findings identify a mechanism whereby, in the face of Aβ25-35 , the concerted actions of ST2-104 leads to a reduction in intracellular calcium overload and inhibition of the CaMKKβ/AMPK/mTOR pathway resulting in attenuation of autophagy and cellular apoptosis. These findings define a mechanistic framework for how ST2-104 transduces “outside” (calcium channels) to “inside” signaling (CaMKKβ/AMPK/mTOR) to confer neuroprotection in AD.
A bi-level model and evolution algorithm for virtual network functions deployment in inter-datacenter elastic optical networks
Authors: Song Erping , Li Hecheng
Published: September 2022

The technology of network function virtualization can effectively reduce the cost of network investment, its key problem is the deployment of virtual network functions, these are transmitted by some data centers. An new model of bi-level optimization is established in this paper, and a hybrid algorithm is proposed by combining a distributed estimation algorithm and differential evolution. Firstly, according to the characteristics of the upper-level variables, the upper-level objectives are optimized by using a binaryencoded distribution estimation algorithm; Then, the differential evolution is used to solve the lower-level problem, and a correlation coefficient is used to select individuals in the design of mutation operator, and in the operation some high-quality individuals are embedded to produce mutation offspring, the search efficiency of the algorithm can be improved in this process. The experimental results on two realworld examples show that the proposed algorithm provides a better deployment scheme.
Iodine maps derived from contrast-enhanced dual-energy computed tomography for operable breast cancer: Correlation of tumoral iodine concentration and visual pattern with pathological features
Authors: Goda Noriko , Funakoshi Mahito , Ono Chiaki , Mukaida Hidenori , Kaneko Mayumi
Published: September 2022

Background: Contrast-enhanced dual-energy computed tomography produces iodine maps (i-maps) based on tissue iodine concentration (IC). We analyzed the features of i-maps in operable breast cancer.
Methods: I-maps made from patients with operable breast cancer were retrospectively reviewed. The mean IC of the whole tumor and visual patterns (sharp/obscure) were analyzed with respect to pathological features. The tumor extent was retrospectively verified with dynamic contrast-enhanced magnetic resonance of mammary gland
(MRM) and pathological specimens.
Results: The median IC of 858 cases was 4.3 (interquartile range [IQR]: 4.0–5.1) mg/mL. The IC of the luminal A-like subtype of invasive breast cancer was significantly higher than that of the human epidermal growth factor 2 (HER2) and triple-negative (TN) subtypes (luminal A-like: 4.5 [IQR: 4.3–5.5] mg/mL vs. HER2: 3.9 [IQR: 3.5–4.4] mg/mL and TN: 3.8 [IQR: 3.6–4.2] mg/mL; both p < 0.05). The IC was significantly correlated with the histological grade and Ki-67 labeling index. Sharp visual patterns correlated with the estrogen receptor and Ki-67 labeling index, while obscure patterns correlated with the HER2 subtype. I-maps underestimated tumor extent in 84 (9.8%) of the 532 partial resection cases, especially in lobular carcinoma and mucinous carcinoma.
Conclusions: The IC and visual patterns correlated with the pathological features of operable breast cancer. Most breast cancers are identifiable on i-maps; however, the adjunction of MRM is preferred for tumors with low IC on i-maps to evaluate tumor extent.
Aesthetic Reconstruction of Onco-surgical Mandibular Defects Using Free Fibular Flap with and without CAD/CAM Customized Osteotomy Guide: A Randomized Controlled Clinical Trial
Authors: Al-Sabahi Mohammed Esmail , Jamali Omer Mohammed , Shindy Mostafa Ibrahim , Moussa Basma Gamal , Amin Ayman Abdel-Wahab , Zedan Mohamed Hamdallah
Published: September 2022

Background
Reconstruction of mandibular defects following ablative surgery remains a challenge even for experienced surgeons. Virtual planning and guided surgery, including computer-aided design/computer-aided manufacturing (CAD/CAM), afford optimized ways by which to plan complex surgery. This study aimed to evaluate and compare aesthetic outcome and surgical efficiency of free fibular flap (FFF) with and without CAD/CAM customized osteotomy guide (COG) for reconstruction of onco-surgical mandibular defects.
Methods
Twenty-two patients indicated for segmental mandibulectomy were randomly assigned to either CAD/CAM with COG group or that without COG- Model based reconstruction (MB group) at a 1:1 ratio. Aesthetic outcomes were evaluated by means of morphometric assessment and comparison for each differential area (DAr) and angle (DAn) in the affected side to the contralateral side of the mandible using computerized digital imaging analysis (CDIA) based on the post-operative 3D CT-scan. Subjective evaluation was performed using the Visual Analogue Scale (VAS) and Patient’s Satisfaction Score (PSS). Surgical efficiency was a secondary outcome and evaluated as total operative time and ischemia time.
Results
The mean sagittal DAr was significantly lower in the COG group (277.28 ± 127.05 vs 398.67 ± 139.10 mm2, P = 0.045). Although there was an improvement in the axial DAr (147.61 ± 55.42 vs 183.68 ± 72.85 mm2), the difference was not statistically significant (P = 0.206). The mean differences (Δ) in both sagittal and coronal DAn were significantly lower in the COG group than in the MB group (6.11 ± 3.46 and 1.77 ± 1.12° vs 9.53 ± 4.17 and 3.44 ± 2.34°), respectively. There were no statistically significant differences in the axial DAn between the two groups (P = 0.386). The PSS was significantly higher in the COG group, reflecting better aesthetic satisfaction than in the MB group (P = 0.041). The total operation and ischemia time were significantly shorter in favor of the COG group with a mean of (562.91 ± 51.22, 97.55 ± 16.80 min vs 663.55 ± 53.43, 172.45 ± 21.87 min), respectively.
Conclusion
The CAD/CAM with COG is more reliable and highly valuable in enhancing aesthetic outcomes and surgical efficiency of mandibular reconstruction by FFF compared to that without COG (MB reconstruction).
Trial registration:
This trial was registered at ClinicalTrials.gov. Registration number: NCT03757273. Registration date: 28/11/2018.
Prioritizing evidence-based practices for acute respiratory distress syndrome using digital data: An iterative multi-stakeholder process
Authors: Ervin Jennifer N. , Dibble Millie R. , Rentes Victor C. , Sjoding Michael W. , Gong Michelle N. , Hough Catherine L. , Iwashyna Theodore J. , Sales Anne E.
Published: September 2022

Background
Evidence-based practices (EBPs) for patients receiving invasive mechanical ventilation vary in the quality of their underlying evidence and ease of implementation.
Research Question:
How do researchers and clinicians prioritize EBPs to help guide clinical decision-making and focus implementation efforts to improve patient care using existing, validated measures?
Study Design and Methods:
We developed a 4-step rapid method using existing criteria to prioritize EBPs associated with lower mortality and/or shorter duration of invasive mechanical ventilation for patients suffering from acute respiratory failure or acute respiratory distress syndrome. Using different types of data including surveys, we (1) identified relevant EBPs, (2) rated EBPs using the Guideline Implementability Appraisal (GLIA) tool, (3) surveyed practicing ICU clinicians from different hospital systems using a subset of GLIA criteria, and (4) developed metrics to assess EBP performance. In this paper, we describe Steps 2 and 3.
Results
In Step 2, we prioritized 11 EBPs from an initial list of 30, using surveys and ratings among a small group of clinician researchers. In Step 3, 42 clinicians from 8 different hospital systems provided assessments of these 11 EBPs which inform the final step of metric development.
Interpretation:
Our prioritization process allowed us to identify 11 EBPs out of a larger group that clinicians perceive are most likely to help optimize invasive mechanical ventilation and improve the outcomes of this vulnerable patient population. While this method was developed in critical care related to adults receiving invasive mechanical ventilation, it is adaptable to other health contexts.
Nomogram for Predicting Spontaneous Pregnancy after Microscopic Varicocelectomy in Infertile Men with Normal Hormone
Authors: Liu Gang , Pan Chunyu , Li Jia , Zhan Yunhong , Bai Song
Published: September 2022

Introduction: The current challenge for the treatment of varicocele is identifying patients who could benefit the most from surgery. We aimed to develop and validate a nomogram for predicting spontaneous pregnancy following microscopic varicocelectomy in infertile men, based on a large cohort.
Methods: Two hundred eighty-two consecutive patients who underwent microscopic varicocelectomy from January 2018 to December 2020 were enrolled as participants in the study. Xiang Hua center (206 patients) as a development cohort. Hu Nan center (76 patients) as a validation cohort. Patient clinicopathologic data were recorded. Multivariate logistic regression was used to build a predictive model with regression coefficients. Then, backward stepwise selection was applied, and the likelihood ratio test with Akaike’s information criterion was used as the stopping rule. The performance of this predictive model was assessed for discrimination, calibration, and clinical usefulness.
Results: Predictors of this model included the age of female partners, diameter of veins, initial and increased total progressively motile sperm count. The model demonstrated good discrimination with an AUROC of 0.925 (p <0.001) and calibration (Unreliability test, p = 0.522) in the validation cohort. Furthermore, the model was clinically useful, according to decision curve analysis.
Conclusions: Our findings indicated that younger female partners, larger diameter of veins, higher initial and increased total progressively motile sperm count were significant predictors of spontaneous pregnancy in infertile men, post microscopic varicocelectomy. This nomogram assists in individual decision-making on the treatment strategy of varicocele preoperatively and may assist in improving the treatment outcome.
A Research of Image Encryption Algorithm Based on Double Time-delay Lorenz System
Authors: Zhou Yuzhen , Zhu Erxi , Li Shan
Published: September 2022

Traditional image encryption technology is usually disadvantaged by low encryption efficiency and inadequate security. In this paper, the time-delay chaos theory was combined with traditional encryption technology to propose a double time-delay chaos-based image encryption algorithm according to the characteristics of image information. First of all, the nonlinear dynamics theory was applied to study the stability of the double time-delay Lorenz system at the equilibrium point and the preconditions for Hopf bifurcation. Then, an analysis was conducted as to the distribution of corresponding characteristic Eq.roots at the equilibrium point, thus obtaining the time-delay critical points for the local asymptotic stability and Hopf bifurcation of the system at the equilibrium point. Secondly, the time-delay critical point was referenced to design the relevant system parameters and time delay of the double time-delay Lorenz system entering the chaotic state, through which the pseudo-random sequence of the chaotic system was determined. Thirdly, the pseudo-random sequence was used for scrambling and diffusion, so as to complete image encryption. Finally, simulation experiment and analysis were conducted for the algorithm from various perspectives such as key space size, key sensitivity, plaintext image sensitivity, plaintext histogram, and so on. According to the research results, the proposed algorithm was capable of producing satisfactory scrambling effect and effective in encrypting and decrypting images without distortion. Meanwhile, the scheme demonstrated not only a certain level of robustness to statistical attack, selective plaintext attack and noise, but also higher stability.