bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of salinity and shaking condition on the growth and virulence of Vibrio parahaemolyticus
Authors: Ngoc My Hanh Tran , Van Nhi Tran , Thi Thu Hoai Nguyen
Published: November 2020

Vibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environment. This organism is the major causative agent of Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Disease (AHPND) which resulted in serious damages to cultured shrimp industry. Understanding the effect of environmental factors on the growth and virulence of this potential pathogen would be beneficial for preventing its outbreak. In this study, the growth and virulence of V. parahaemolyticus was examined under different salinity and shaking condition. V. parahaemolyticus XN9 was cultured in Brain Heart Infusion (BHI) medium with different sodium chloride concentrations (2.0, 2.5 and 3.0%) and different shaking conditions (0, 120 and 240 rpm). The growth of the bacterium was recorded over 8h and six extracellular enzymes of V. parahaemolyticus XN9 including caseinase, hemolysin, lecithinase, lipase, gelatinase, chitinase were investigated using agar-based method. The growth of V. parahaemolyticus was varied among different salinity and shaking conditions. It showed the best growth at 2.0% NaCl and 240 rpm. No change in the enzymatic activity (EA) of the tested extracellular enzymes was observed while changing salinity except the significant decline of gelatinase from 3.49±0.19 to 2.77±0.17 mm following salinity increase (p < 0.05). On the other hand, regarding shaking condition, lipase was the one to increase its activity significantly following the increase of shaking speed (p < 0.05). While caseinase, lecithinase, gelatinase and lipase were well expressed in V. parahaemolyticus, no hemolytic and chitinase activity was observed in any tested conditions. In summary, our study showed that 2.0 % NaCl and 240 rpm shaking promoted the best growth of V. parahaemolyticus and resulted in highest activity of gelatinase and lipase in this bacterium.
Cloning and expression of maltooligosyltrehalose trehalohydrolase from Sulfolobus solfataricus DSM 1616 in Bacillus subtilis WB800
Authors: Tien Cuong Nguyen , Thi Hien Trang Nguyen , Thi Thao Nguyen , Thanh Hoang Le , Sy Le Thanh Nguyen , Thi Anh Tuyet Nguyen , Manh Dat Nguyen , Duc Manh Le , Thi Thanh Huyen Do , Thi Thu Nguyen , Thi Trung Nguyen , Thi Yen Hoang , Thi Tuyen Do
Published: November 2020

Maltooligosyltrehalose trehalohydrolase (MTHase) is an industrial enzyme for the production of trehalose. A DNA fragment of 1680 bp encoding for MTHase was cloned from Sulfobolus solfataricus DSM 1616 then fused with promoter acoA-amyE already amplified from pMSE3 vector by PCR to generate an expression cassette acoMTH. Afterward the cassette was inserted into pAC7 vector for expression of the gene in Bacillus subtilis WB800 – a conventional expression system. Gene MTH was inserted into the genome of B. subtilis WB800 by cross-exchange event of pAC7 vector with the host genome for expression of high quality and high quantity of extracellular recombinant protein. By crossing-exchange event at 3’amyE-5’amyE, the expressional cassette was integrated into B. subtilis WB800 genome. The expressional cassette was integrated into B. subtilis WB800 genome replacing 3’amyE-5’amyE, hindering the native amylase activity of the host. Expression of expected protein was confirmed by electrophoresis SDS-PAGE. From our results, it indicates that gene MTH was expressed successfully in B. subtilis WB800. After 0.5% acetoin induction for 48 h, the data showed that the protein with a molecular mass of ~64 kDa on SDS-PAGE was expressed. The level of recombinant protein in WBpAacoMTH was increased and reached 2.5%, 15.2% and 21.95%, respectively comparing with native B. subtilis WB800.
Study on genetic variations of the D-loop region in three Vietnamese ethnic groups Kinh, Lolo and Lahu
Authors: Duong Nguyen Thuy , Van Phong Nguyen , Thy Ngoc Nguyen , Van Hai Nong
Published: November 2020

Mitochondrial DNA (mtDNA) sequence analysis has been widely used to investigate population genetic and evolutional history of different ethnic groups worldwide. In this study, the D-loop region of 119 Vietnamese individuals belonging to three different ethnic groups was sequenced and compared with reference mtDNA on rCRS for genetic variations. Total 218 genetic polymorphisms were found in this population, among which 48 variations appeared with frequencies of more than 0.1. Further statistical analysis showed that there were 23, 13 and 24 single nucleotide polymorphisms (SNP) distributed differently between Kinh vs. Lolo, Kinh  vs. Lahu , and Lolo  vs. Lahu groups, respectively. The mean pairwise genetic distances between each pair of ethnic groups were 0.0101, 0.0098 and 0.0092 for Kinh - Lolo, Kinh - Lahu, Lolo - Lahu, respectively. This indicated that although the Lolo and Lahu ethnic groups both belong to the Tibeto-Burman language group, the genetic distance between them was no closer than the genetic distances between them and the Kinh group of the Viet-Muong (Austroasiatic) language group. This study was the first research on the D-loop region of the Lolo and Lahu populations in Vietnam. These results provided more data for exploring the genetic background and the history of those ethnic groups as well as other minority ethnic groups in Vietnam.
Genetic testing identifies the potential risk of multiple endocrine neoplasia in a Vietnamese family
Authors: Hai Ha Nguyen , Thi Thanh Hoa Nguyen , Thi Dung Pham , Huy Binh Nguyen , Dang Ton Nguyen
Published: November 2020

Multiple endocrine neoplasia type 2A (MEN2A) is a rare syndrome characterized by the presence of medullary thyroid carcinoma, pheochromocytoma, hyperparathyroidism and sometimes cutaneous lichen amyloidosis. This syndrome is caused by a germline activation mutation in the rearranged during transfection (RET) proto-oncogene transmitted by an autosomal dominant inheritance. In this study, we reported a rare case of a 44-year-old man from Vietnam with medullary thyroid carcinoma and pheochromocytoma as the symptom of MEN2A. Genetic testing indicated a nucleotide substitution located in exon 11 of the RET proto-oncogene (c.1900T>C, p.C634R), which was reported as a known pathogenic mutation of MEN2A. Further genetic tests on the other family members found the same mutation in his daughter (currently 14 years-old) and his son (currently 8 years-old). Although these 2 children do not yet have any manifestations of MEN2A, this data emphasizes their high risks of this disease. Therefore, this case draws attention to the importance of genetic counselling in C634R carriers, as well as rigorous follow-up appointments to reduce incidence and mortality since the mutation is classified as a high-risk group within the medullary thyroid carcinoma guidelines.
Intracerebral hemorrhage: update and future directions
Authors: ROCHA Eva , ROUANET Carolina , REGES Danyelle , GAGLIARDI Vivian , SINGHAL Aneesh Bhim , SILVA Gisele Sampaio
Published: November 2020

ABSTRACT Intracerebral hemorrhage (ICH), defined as bleeding into the brain parenchyma, is a significant public health issue. Although it accounts for only 10 to 15% of strokes, it is associated with the highest morbidity and mortality rates. Despite advances in the field of stroke and neurocritical care, the principles of acute management have fundamentally remained the same over many years. The main treatment strategies include aggressive blood pressure control, early hemostasis, reversal of coagulopathies, clot evacuation through open surgical or minimally invasive surgical techniques, and the management of raised intracranial pressure.
Transitional care from hospital to home for older people: implementation of best practices
Authors: Costa Maria Fernanda Baeta Neves Alonso da , Sichieri Karina , Poveda Vanessa de Brito , Baptista Cleide Maria Caetano , Aguado Priscila Cantoni
Published: November 2020

ABSTRACT Objective: to assess the conformity of nursing care concerning best evidence in transitional care from hospital to home for older people. Methods: a project to implement best evidence based on the model proposed by the Joanna Briggs Institute in surgical clinic of a university hospital with older people, caregivers or family members, and nurses, between July and August 2019. Eight evidence-based criteria have been audited through interviews, medical records and computerized system, presented in percentages. Results: the highest non-compliance rate found in a baseline audit was absence of continued training on transitional care and hospital discharge plan. Identifying barriers to best practices included educational programs; afterwards, there was an improvement in compliance rates in all the criteria assessed. Final considerations: the criteria based on audited evidence showed an increase in compliance rates with the strategies implemented, contributing to improving transitional care for older people.
A erradicação da poliomielite em quatro tempos
Authors: Verani José Fernando de Souza , Laender Fernando
Published: November 2020

O objetivo deste artigo é rever o “estado da arte” dos avanços, obstáculos e estratégias para atingir a erradicação global da pólio. As ações de controle da poliomielite iniciaram na década de 1960 com o advento das duas vacinas antipoliomielíticas, a vacina oral da pólio (VOP) e a vacina inativada da pólio (VIP). No período de 1985 a 2020, são implementadas estratégias para atingir a meta de erradicação do poliovírus selvagem (WPV). Após o sucesso da interrupção da transmissão autóctone do WPV na região da Américas, foi lançada a meta da erradicação global. Descrevemos o processo de erradicação em quatro tempos: (1) O advento das vacinas VIP e VOP iniciou a era do controle da poliomielite; (2) A utilização massiva e simultânea da VOP teve impacto significativo sobre a transmissão do poliovírus selvagem no final da década de 1970 no Brasil; (3) Políticas públicas (nacionais e internacionais) decidem pela erradicação da transmissão autóctone do poliovírus selvagem nas Américas e definem as estratégias epidemiológicas para interromper a transmissão; e (4) A implantação das estratégias de erradicação interrompeu a transmissão autóctone do WPV em quase todas as regiões do mundo, exceto no Paquistão e Afeganistão, onde, em 2020, cadeias de transmissão do WPV1 desafiam as estratégias de contenção do vírus. Por outro lado, a persistência e a disseminação da circulação do poliovírus derivado da VOP, em países com baixa cobertura vacinal, somadas às dificuldades para substituir a VOP pela VIP constituem, atualmente, os obstáculos para a erradicação a curto prazo. Finalmente, discutimos as estratégias para superar os obstáculos e os desafios na era pós-erradicação.
Jaffe-Lichtenstein Syndrome Associated with a Simple Bone Cyst: Unprecedented Rare Case Report
Authors: Miranda Poliana Gonçalves , Henriques João César Guimaraes , Paulo Luiz Fernando Barbosa de , Guedes Cizelene do Carmo Faleiros Veloso , Mitri Fabio Franceschini , Silva Cláudia Jordão
Published: November 2020

Abstract Lesions denominated fibro-osseous lesions of the jaw constitute a diversified group of disorders, in which the normal bone architecture is replaced by fibroblasts, collagen fibers and immature bone. At present, the World Health Organization recognizes four variants of these lesions, namely: bone-cement dysplasia, fibrous dysplasia, ossifying fibroma and Familial gigantiform cementoma. Fibrous dysplasia may present in the monostotic form, affecting one single bone or an isolated craniofacial region; and in the Polyostotic form, involving two or more bones of the skeleton, and eventual association with syndromic conditions. The patient, C.P.G., 43 years old, sought attendance due to symptomatic increase in the region of the mandibular body on the right side. Imaging exams revealed craniofacial areas with ground-glass aspect, beyond the extensive mandibular radiolucent lesion. During the physical exam, spots of the Café au lait type disposed on the right side of the body were identified, in addition to uncoordinated gait with distinct shortening of the right leg. Additional radiographic exams showed evidence of skeletal dissemination of the disease. The patient denied any sexual precocity, and the final diagnosis was fibrous dysplasia, expressed by means of the Jaffe-Lichtenstein syndrome, in association with a simple bone cyst.
Physicochemical Properties of a Bioceramic Repair Material - BioMTA
Authors: Coaguila-Llerena Hernán , Ochoa-Rodriguez Victor Manuel , Castro-Núñez Gabriela Mariana , Faria Gisele , Guerreiro-Tanomaru Juliane Maria , Tanomaru-Filho Mario
Published: November 2020

Abstract This study aimed to assess the physicochemical properties of a repair material in the Brazilian market, BioMTA, in comparison to other two materials currently in use (Biodentine and MTA Angelus). The initial setting time was evaluated using Gillmore needle. The pH was measured with a pH-meter after 24 h, 3, 7, 14 and 21 days. The radiopacity was determined using the equivalence in millimeters of aluminum (mm Al) from digitized occlusal radiographs. Solubility was determined after immersion in water for 7 days. The data were analyzed by one-way ANOVA and Tukey tests (a=0.05). The BioMTA initial setting time (5.2 min) was lower than the other materials (p<0.05). All materials showed an alkaline pH at 21 days. At 24 h, BioMTA was the most alkaline material (p<0.05); and at 3, 7, 14 and 21 days there was no difference between BioMTA and Biodentine (p>0.05), both being more alkaline than MTA Angelus (p<0.05). The radiopacity of BioMTA (4.2 mm Al) was significantly higher compared to Biodentine (p<0.05) and lower than MTA Angelus (p<0.05). The solubility of the materials was -4.2%, -1.6% and 4.1% for BioMTA, MTA Angelus and Biodentine, respectively, with a significant difference between them (p<0.05). Therefore, it can be concluded that BioMTA displayed a shorter setting time, an alkaline pH, a higher radiopacity, and a gain in mass.
Filling Ability and Flow of Root Canal Sealers: A Micro-Computed Tomographic Study
Authors: Pinto Jáder Camilo , Torres Fernanda Ferrari Esteves , Pivoto-João Mariana Mena Barreto , Cirelli Joni Augusto , Guerreiro-Tanomaru Juliane Maria , Tanomaru-Filho Mário
Published: November 2020

Abstract This study evaluated by micro-computed tomography (μCT) the filling ability in curved root canals, besides the flow of AH Plus (AHP) and Neo MTA Plus (NMTAP) sealers using different methodologies. Mandibular molars mesial roots with two root canals and degree of curvature between 20° and 40° were selected. The specimens were prepared with the ProDesign R system up to size 35.05 and were filled with the sealers by a continuous wave of condensation technique, Thermo Pack II (n=12). The teeth were scanned using μCT after root canal preparation and obturation. The volumetric percentage of filling material and voids were calculated. Flow was evaluated based on ISO 6876/2012 (n=10). Flow and filling were also evaluated in μCT using a glass plate with a central cavity and four grooves from the central cavity (n=6). Flow was linearly calculated into the grooves. The central cavity filling (CCF) and lateral cavity filling (LCF) were calculated in mm³. Data were submitted to non-paired t test with a significance threshold at 5%. The percentage of filling and voids between the root canals filled with AHP or NMTAP was similar (p>0.05). NMTAP presented the lowest flow in conventional test (p<0.05). Using μCT, sealers had similar CCF, LCF and linear flow (p>0.05). In conclusion, NMTAP and AHP had similar filling ability in curved mesial root canals of mandibular molars without presence of isthmus. Although AHP presented better flow than NMTAP using ISO methodology, there was no difference between these materials regarding volumetric filling when evaluated by μCT.
Interfacial Stress and Bond Strength of Bulk-Fill or Conventional Composite Resins to Dentin in Class II Restorations
Authors: Duarte Júlio César Lemos , Costa Ana Rosa , Veríssimo Crisnicaw , Duarte Renata Webster , Calabrez Filho Saturnino , Spohr Ana Maria , Borges Gilberto Antonio , Correr-Sobrinho Lourenço
Published: November 2020

Abstract The aim was to evaluate the microtensile bond strength (mTBS) to dentin and interfacial stress in a class II cavity restored with bulk-fill or conventional composite resins and the margin interfaces. Vertical slot class II cavities in the mesial face, with the gingival end in dentin, were prepared in 72 third molars, being divided into groups (n=24): G1-Tetric N-Ceram; G2-Tetric N-Ceram Bulk-Fill; G3-SonicFill. Clearfil SE Bond adhesive system was used in all groups. Half of the teeth in each group (n=12) were submitted to thermo-mechanical cycling (TMC). Restored teeth (n=9) were cut perpendicular to obtain beams, which were submitted to a mTBS test in an EMIC machine. The cervical margins in dentin of the restored teeth (n=3) were assessed using SEM through epoxy resin replicas as well as the section of the restoration. Interfacial stresses after load application were calculated by 2D finite element analysis. The mTBS means-MPa followed by different letters represent statistical difference by ANOVA and Games-Howell’s test (p<0.05): Without TMC: G1-15.68±6.10a; G2-10.08±5.21ab; G3-7.98±3.76b. With TMC: G1-9.70±5.52a; G2-5.79±1.42a; G3-4.37±1.87a. Interfacial stress (MPa) was 4.4 for SonicFill, 3.9 for Tetric N-Ceram, and 3.5 for Tetric N-Ceram Bulk-Fill. SEM images showed continuous margins for all composite resin restorations. It was possible to conclude that SonicFill obtained a slightly higher interfacial stress and lower bond strength to dentin in comparison with Tetric N-Ceram and Tetric N-Ceram Bulk-Fill. Continuous margin interfaces were obtained for Tetric N-Ceram, Tetric N-Ceram Bulk-Fill, and SonicFill. However, voids were observed in the SonicFill restorations.
Strategies for Potential Toughening of Acrylic Denture Bases Polymerized With Microwave Energy
Authors: Consani Rafael Leonardo Xediek , Paula Andreia Bolzan de , Fugolin Ana Paula Piovezan , Pfeifer Carmem S.
Published: November 2020

Abstract Thiourethane additives have been shown to improve properties in several dental polymer applications. The aim of this study was to verify the effect of the addition of thiourethane oligomers and acrylamide or isobornyl-based plasticizers on the physical properties of the denture base acrylic resin polymerized with microwaves. Thiourethane oligomer (TU) was synthetized and added to microwaved acrylic resin in proportions varying between 3 and 14 wt%. Separate experimental groups included the addition of dimethyl acrylamide (DMAM) and isobornyl methacrylate as plasticizers, at concentrations varying from 5 to 20 wt%. Samples were polymerized using microwave energy at 500 Watts for 3 min, deflasked at room temperature, stored in water at 37 °C for 24 h, and evaluated for: linear dimensional change, gloss, Knoop hardness, surface roughness, impact strength, yield strength, elastic modulus, toughness, yield strength, viscosity, glass transition temperature and network heterogeneity, and water sorption/solubility. Data were analyzed with ANOVA/Tukey’s post-hoc test (a=5%). The addition of TU led to properties that were similar or worse than the materials to which it was not added, except for dimensional stability. The impact on properties was statistically significant for all materials above 20% addition of TU. The addition of DMAM at 5 wt% or isobornyl methacrylate at 10 wt% improved yield strength and modulus, but increased water sorption and solubility. Except for dimensional stability, the addition of thiourethane oligomers to acrylic denture base materials compromised most tested properties. The use of DMAM and isobornyl methacrylate improved properties for selected compositions.
Residues of glyphosate and aminomethylphosphonic acid (AMPA) in genetically modified glyphosate tolerant soybean, corn and cotton crops
Authors: Rodrigues Nadia Regina , Souza Ana Paula Ferreira de , Morais Pedro Patric Pinho , Braga Daniella Pascon Vianna , Crivellari Augusto Cesar , Favoretto Luís Roberto Graça , Berger Geraldo Ubirajara
Published: November 2020

ABSTRACT: Glyphosate is a broad-spectrum herbicide capable of controlling a wide range of weed. It uses could cause the occurrence of residues in conventional or genetically modified crops. For this purpose, grains (soybean and corn) and cotton seeds were sampled from commercial areas in 2012/2013 to 2017/2018 seasons in different Brazilian agricultural regions to monitor glyphosate residues levels and its metabolite (aminomethylphosphonic acid - AMPA) after different managements. The glyphosate residues levels in genetically modified corn (GM) ranged from no detected (ND) to 0.15 mg kg-1, in GM soybean values ranged from ND to 2.81 mg kg-1 and in GM cotton ranged from ND to 1.78 mg kg-1. AMPA residues levels indicated a correlation with the glyphosate residues. Glyphosate residues levels in soybean and corn grains and cotton seeds were within the Maximum Residue Limits (MRLs) established by ANVISA and Codex Alimentarius.
Sustainability of carrot-cowpea intercropping systems through optimization of green manuring and spatial arrangements
Authors: Silva Josimar Nogueora da , Bezerra Neto Francisco , Lima Jailma Suerda Silva de , Chaves Aridênia Peixoto , Nunes Renato Leandro Costa , Rodrigues Gardênia Silvana de Oliveira , Lino Vitor Abel da Silva , Sá Jolinda Mércia de , Santos Elizangela Cabral dos
Published: November 2020

ABSTRACT: One of the biggest challenges in intercropping of two or more crops is to maintain their productivity as that of their sole counterparts. In order to obtain sustainable intercropping systems in a semi-arid environment, this study evaluated the agro-economic advantage and sustainability of intercropped systems of carrot and cowpea, optimizing the green manuring and spatial arrangements. Experiments were conducted in the period from July to October 2017 and 2018, using a randomized complete block design with the treatments arranged in a 4 x 3 factorial scheme, with four replications. Treatments included four amounts of M. aegyptia incorporated into the soil (20, 35, 50, and 65 t ha-1 on dry basis) with three spatial arrangements (2:2, 3:3, and 4:4) between the components cultures formed from cowpea rows alternated with carrot rows. The agro-economic advantage indices evaluated in the intercropping systems were: land equivalent ratio (LER), area-time equivalent ratio (ATER), score of the canonical variable (Z), actual yield loss (AYL), intercropping advantage (IA), gross income (GI), net income (NI), rate of return (RR), and profit margin (PM). The highest agro-economic advantages of the carrot and cowpea intercropping were obtained for LER, ATER, NI, and RR of 2.60, 4.16, US$ 8,720.99 ha-1, and US$ 2.21, respectively, for M. aegyptia biomass amounts of 46.42, 47.82, 32.60 and 31.51t ha-1 added to the soil. The 2: 2 spatial arrangement was the one with the best productive performance, with higher net income and rate of return in the intercropping of carrot-cowpea.
Evaluating the effectiveness of university agricultural extension test stations using Wuli-Shili-Renli methodology
Authors: Liu Tingting , Tao Peijun
Published: November 2020

ABSTRACT: Wuli-Shili-Renli (WSR) methodology was used to assess the effects of systematic management on university agricultural extension test stations, in order to understand areas for further optimization. We developed a framework model and evaluation index system, of which, the weights of the evaluation index system were determined using AHP. Using an agricultural extension test station of Hebei Agricultural University as an example, we interviewed and surveyed in total of 225 university extension experts from nine test stations. Assessment showed that the test station system organization produces desirable societal benefits and improvements. In addition, the organizational structure and job allocation were found to act as high-impact factors in improving the optimal system function, with the university extension experts functional better in the incentive and guarantee mechanism than other factors. Our investigation validated that the university extension experts were critical in designing the incentives and evaluation programs.
Factors effective on reaching goals and target groups of agricultural supports: a case study in Diyarbakir Province, Turkey
Authors: Akin Songül , Kara Abdurrahman
Published: November 2020

ABSTRACT: In this study, factors affecting the willingness to continue organic almond production in Eğil and Dicle districts of Diyarbakır were investigated. A semi-structured interview method was used in data collection. Descriptive statistics and logistic regression were used in data analysis. According to the results, it was reported that the association membership status and agricultural supports are very important (P<0.01) and the size of the almond establishment (acres) (p<0.01). Association membership status significantly increases the desire to continue organic production by approximately 11%. Conversely, 24.2 percent of the participants who claim that support is needed for almond production is willing to produce organic. However, one hectare increase in almond plantation reduced the probability of continuing organic almond production in the ceteris paribus by 1%, suggesting that organic farming awareness is not sufficiently developed. It turns out that support payments are the main driving force for farmers to continue organic production, and the underlying reason for association membership is access to support payments.
Principal component analysis of steers performance and structural and nutritional characteristics of Mombasa grass
Authors: Gurgel Antonio Leandro Chaves , Difante Gelson dos Santos , Montagner Denise Baptaglin , Euclides Valeria Pacheco Batista , Araujo Alexandre Romeiro de , Santana Juliana Caroline Santos
Published: November 2020

ABSTRACT: There is little information about whether the increased secondary productivity observed in pastures fertilized with high nitrogen rates is attributable to fluctuations in the nutritional value or pasture structural characteristics, or both. This study aimed to identify a set of factors (structural and nutritional characteristics) that best explain the performance of beef steers grazing Mombasa grass pastures under residual effects of nitrogen fertilizer. The data were collected in Mombasa grass pastures fertilized with increasing rates of nitrogen (N) (100, 200 and 300 kg ha-1) from 2015 to 2017. In 2018, nitrogen fertilization was not used in order to characterize a residual effect of the nutrient. Variables related to pasture structural characteristics such as forage accumulation rate (FAR), canopy height, forage mass (FM) and morphological components were evaluated. The study also evaluated the nutritional value of leaf blades and the performance of beef steers based on average daily gain (ADG) and stocking rate. Principal component analysis was performed using the dataset available. Most of the variance (99.6%) was explained by only two principal components (PCs), of which 90.0% corresponded to PC1. The most influential parameters for PC1, in order of priority, were: FAR, FM, leaf blade and stem masses. These variables were positively associated with stocking rate. Conversely, ADG was not associated with any variable. ADG was the most relevant variable for the second PC; however, this PC explained less variance (9.6%). The structural characteristics of the pasture (FAR, FM and morphological components mass) better explain the fluctuations in the performance of cattle on pastures of Mombasa grass under residual effects of nitrogen fertilizer. The stocking rate is an efficient parameter to support decision-making in managed pastures with variable stocking.
Seed moisture content can be used to accelerate dormancy release during after-ripening of Urochloa humidicola cv. Llanero spikelets
Authors: Abrantes Fabiana Lima , Machado-Neto Nelson Barbosa , Custódio Ceci Castilho
Published: November 2020

ABSTRACT: Increased demand for livestock products is leading to research on more productive pastures adapted to different environments. Urochloa humidicola is a species adapted to low-fertility conditions and to environments occasionally subjected to temporary flooding and with highly dormant seeds at harvest. Mechanical and sulphuric acid (H2SO4) scarification are used to release these seeds from dormancy; although, there are several side effects. Hydrogen peroxide (H2O2) is linked to dormancy release in many crop species. The objective of this research was to characterize the dormancy release and H2O2 accumulation during one year of storage in dormant spikelets of U. humidicola cv. Llanero equilibrated to 4.5% and 50% relative humidity (RH) and stored at 20 °C, during which time the seeds had moisture contents (MCs) of 0.03 and 0.1 g H2O g-1 dry weight (DW), respectively. The seeds were evaluated initially and at 3, 6 and 12 months; the seeds from spikelets or just caryopses were evaluated. Germination and dormancy percentage, H2O2 content, superoxide dismutase (SOD) and peroxidase (PRX) activity were determined. The germination and H2O2 content increased mainly in the caryopses, and SOD activity increased. Moreover, dormancy and PRX activity decreased during storage. For the first time, it was shown, in U. humidicola cv. Llanero that dormancy release during storage is caused mainly by the accumulation of reactive oxygen species (ROS) in the spikelets even under ultra-dry storage conditions (4.5% RH), and this process could be accelerated by seed storage at a MC of 0.1 g H2O g-1 DW at 20 °C.
Mycoplasma spp. in captive snakes (Boa constrictor and Bothrops atrox) from Brazil
Authors: Magalhães Barbara Souza Neil , Machado Leandro dos Santos , Figueira Arthur de Almeida , Dias Thomas Salles , Feijó Thiago de Almeida , Barreto Maria Lucia , Tuffanelli Giulia de Almeida , Cunha Nathalie Costa da , Nascimento Elmiro Rosendo do , Pereira Virginia Léo de Almeida , Almosny Nádia Regina Pereira
Published: November 2020

ABSTRACT: Although rare, mycoplasmas are included among the causes of respiratory diseases in reptiles and, in the order Squamata, three reports of these microorganisms causing diseases in pythons have already been reported. This study aimed to evaluate the occurrence of Mycoplasma species in captive snakes. A total of 26 snakes of the families Pythonidae (13), Boidae (7), Viperidae (5) and Colubridae (1) from RioZoo, Brazil, were evaluated. Animals were examined to determine clinical signs consistent with any infectious disease. Tracheal swab samples from snakes were collected in Frey medium and analyzed for the presence of Mycoplasma spp.by isolation and a genus-specific PCR. DNA sequencing analyses of six positive samples by PCR were carried out to identify the species. Using isolation 19.23% (5/26) was positive, while 65.38% (17/26) of the animals were positive by PCR. Based on the analyses of the six sequences obtained, there was similarity with a Mycoplasma spp. previously described in a phyton and, M. agassizii and M. testudineum reported in chelonians. This is the first report of Mycoplasma spp. in animals of the families Boidae and Viperidae. Mycoplasma spp. were detected in snakes with and without clinical signs. The mycoplasmas reported resented identity (range, 95% to 100%) to others already described in reptiles. There was no relationship between the presence of Mycoplasma spp. and clinical signs.
Translanguaging as a Tool for Decolonizing Interactions in a Space for Confronting Inequalities
Authors: Liberali Fernanda , Swanwick Ruth
Published: November 2020

ABSTRACT Taking into account the 2030 Agenda for Sustainable Development, from the UN, this article addresses the construction of artistic contexts where students, teachers, principals, coordinators, sign language interpreters, artists, and researchers work together with the Sustainable Development Goal 10 - Reduce inequality within and among countries, in a Project entitled Digitmed Program. This project gathers deaf and hearing, migrants and Brazilian participants from very poor and wealthy communities in discussions about the development of interdisciplinary curriculums, which aim at de-encapsulation of ideas, contents, roles, perspectives, grades, languages spoken, economic background, among others. In this article, the involvement of deaf and hearing participants in the interdisciplinary work with poems as a form of art for resistance will be described. The potential of translanguaging is analysed as a revolutionary possibility for tackling inequality and marginalisation.
Marx, Vygotsky and Freire: methodological discussions on the role of language in social transformation
Authors: Jones Peter E , Magalhães Maria Cecília C
Published: November 2020

ABSTRACT This paper offers a Marxist grounding for a liberatory, critical-collaboratory dialogic praxis in educational contexts and examines the implications of such praxis for an understanding of the potential role of the school as a site of critical thinking. Aligning with Stetsenko’s ‘Transformative-Activist Stance’, the discussion centres on clarifying the methodological rationale for approaching language as a means of cultural action and social transformation based on Marx’s materialist conception of history and the educationally based dialogical approaches of Vygotsky and Freire.
Yield and nitrogen balance in corn grown under no-tillage system
Authors: Silva Helton de S. , Souza Adailson P. de , Carvalho Mary A. B. de , Silva Valéria B. da , Barbosa Ewerton da S. , Santos Michelly F. dos
Published: November 2020

ABSTRACT The corn crop is highly responsive to nitrogen fertilization; however, the application of high doses generally causes a reduction in the use efficiency of fertilizer. In this sense, this study aimed to determine the yield and nitrogen balance in corn grown under the no-tillage system. The experiment consisted of the application of four nitrogen doses (0, 30; 70 and 95 kg ha-1). In all plots with the AG 1051 hybrid corn, 80 and 56 kg ha-1 of P2O5 and K2O were applied, respectively. The experiment was arranged in a randomized block design with five replications. The grain yield had a linear increasing behavior according to the nitrogen doses, which reached 5,684.79 kg ha-1. The fertilization efficiency and the N extraction increased linearly with the increase of the nitrogen doses, obtaining with the highest dose the extraction of 114.93 kg ha-1 of N and a fertilization efficiency of 65%. The soil N stock of 4,489 kg ha-1 provides corn plants with 52.81 kg ha-1 of this nutrient. Corn adjusts the biomass production according to the availability of nitrogen, with no effect of N doses on the nutrient content in plant tissues.
Maize yield influenced by the residual effects of sedimentary phosphates in high-calcium soil
Authors: Souza Richard M. de , Sobral Lafayette F. , Oliveira Junior Adilson de
Published: November 2020

ABSTRACT It was evaluated the residual effects of sedimentary phosphates associated with the annual application of phosphate on maize grown in Inceptisol soil with a high exchangeable calcium concentration and pH value of 6.0. The experiment was conducted based on a completely randomized block design with strip-split plots. The main plots were treated with Bayóvar rock phosphate, Itafós rock phosphate, or triple superphosphate, while the control received no additional phosphate. The phosphate sources were applied by broadcasting and incorporated in the soil two years prior to the current study at 200 kg of P2O5 ha-1, with no tillage in subsequent years. In the sub-plots, phosphate doses of 0, 60, and 120 kg of P2O5 ha-1 year-1, as triple superphosphate, were applied at the base of the sowing furrows. Leaf phosphorus (P), grain yield, and soil P by ion exchange resin were evaluated. Differences were observed between the leaf P among the plots treated with phosphate sources and the control plot, which declined from 2013 to 2015. In 2013 and 2014, rock phosphate residuals influenced the grain yield when there was no annual application of phosphate. In 2015, grain yields in rock phosphate treatments without annual phosphate application were not superior to those in the control treatment and did not differ significantly from the plots receiving triple superphosphate. Furthermore, it was found that the soil P content extracted by ion exchange resin was higher in the Itafós treatment; however, for this source, the correlation between soil P and grain yield was relatively weak.
Growth and yield of gherkin cultivars in coconut fiber fertigated with nutrient solutions
Authors: Oliveira Francisco de A. de , Oliveira Mychelle K. T. de , Santos Sandy T. dos , Costa Jessilanne P. B. M. , Medeiros José F. de , Régis Lúcia R. de L.
Published: November 2020

ABSTRACT Substrate cultivation has been gaining interest from vegetable producers, however technical information for many crops is still recent, such as the gherkin, especially regarding the nutrient solution. This study was developed with the objective of evaluating the performance of three gherkin cultivars (Do Norte, Liso de Calcutá and Liso Gibão) grown in coconut fiber and fertigated with nutrient solutions of different concentrations (50, 75, 100 and 125% of the recommendation for melon), using a randomized block design, in a 3 x 4 factorial scheme, with four repetitions and plots composed of three plants. Growth and production variables were evaluated. In general, cv. Liso de Calcutá produced heavier fruits (35.69 g fruit-1), however cv. Liso Gibão produced an average of 21.6 fruits plant-1, being more productive (542.19 g planta-1). The cultivars Do Norte and Liso de Calcutá were less demanding on nutrients. In order to obtain the highest production of gherkin fruits cultivated in coconut fiber, fertigations must be carried out using nutrient solutions diluted in 50% for the cultivars Do Norte and Liso de Calcutá, while for cv. Liso Gibão it is recommended to use nutrient solution diluted in 75% of the concentration of nutrients recommended for the cultivation of melon.
Papel do Instrutor de Pediatric Advanced Life Support na Aplicação das Melhores Práticas em Ressuscitação Pediátrica
Authors: Gaspary Lisiane Valdez , Paiva Edison Ferreira de
Published: November 2020

Resumo: Introdução: A parada cardiorrespiratória é um evento emergencial que requer tanto a identificação precoce de sinais de deterioração clínica quanto ações rápidas e eficazes para a sua reversão. Muitos médicos e enfermeiros que atendem crianças procuram se capacitar no atendimento desses eventos, realizando o curso Pediatric Advanced Life Support (PALS), tornando-se posteriormente instrutores. O objetivo desta pesquisa foi conhecer a percepção do instrutor de PALS sobre seu papel na articulação entre o cenário simulado e o real da ressuscitação pediátrica. Método: Trata-se de uma abordagem qualitativa em que se utilizou a entrevista semiestruturada como técnica de pesquisa. Foram realizadas 12 entrevistas com instrutores do PALS vinculados a variados centros de treinamento em São Paulo. Os dados qualitativos das entrevistas seguiram o método de análise proposto por Minayo, e o marco teórico adotou os conceitos de campo e habitus de Pierre Bourdieu. Resultados: A análise das entrevistas mostrou que os médicos e enfermeiros se sentem reconhecidos e seguros, conduzem melhor a equipe, realizam intervenção construtiva, sugerem mais treinamentos em ressuscitação pediátrica e implantam melhorias nos serviços após se tornarem instrutores do PALS. O habitus incorporado no campo simulado foi reproduzido pelos instrutores no campo da assistência, exceto na aplicação do debriefing. Conclusão: O papel dos instrutores foi de multiplicação das boas práticas, envolvendo as equipes e favorecendo a realização de atendimentos sistematizados e baseados em evidências, com resultados positivos no dia a dia das instituições hospitalares.
Tendência temporal e distribuição espacial da mortalidade por acidentes de trânsito no Piauí, 2000-2017
Authors: Sousa Roniele Araújo de , Sousa Cyntia Meneses de Sá , Silva Flávia Raymme Soares e , Rodrigues Malvina Thaís Pacheco , Cardoso Osmar de Oliveira , Mascarenhas Márcio Dênis Medeiros
Published: November 2020

Resumo Objetivo: Analisar a tendência temporal e a distribuição espacial da mortalidade por acidentes de trânsito (AT) no Piauí, de 2000 a 2017. Métodos: Estudo ecológico de séries temporais, com dados do Sistema de Informações sobre Mortalidade (SIM), referentes à mortalidade por AT em residentes do estado do Piauí, segundo sexo, faixa etária e município. Empregou-se regressão de Prais-Winsten. Resultados: Registraram-se 14.396 óbitos, com taxa de mortalidade por 100 mil habitantes de 13,9 em 2000 e 30,6 em 2017. Houve aumento significativo na taxa de mortalidade por AT (variação percentual anual [VPA] de 6,4% – IC95% 4,3;8,7), maior entre motociclistas (VPA=14,7% – IC95% 9,7;20,0) e entre ocupantes de veículos (VPA=15,2 – IC95% 10,5;20,2). Conclusão: Houve aumento significativo da mortalidade por AT no Piauí, principalmente de motociclistas e ocupantes de veículos. São necessárias ações para promover a segurança viária e prevenir mortes no trânsito.
Laboratory investigation into the monotonic and cyclic behaviour of a clean sand stabilised with colloidal silica
Authors: Vranna Antigoni , Tika Theodora , Papadimitriou Achilleas
Published: November 2020

This paper presents a laboratory investigation into passive stabilisation of liquefiable sands by means of colloidal silica (CS). In order to examine the improvement of the mechanical behaviour of liquefiable sands stabilised with CS, an extensive laboratory testing programme comprising unconfined compressive strength and Brazilian tensile strength tests, as well as undrained monotonic and cyclic triaxial tests, was performed on a clean quartz sand treated with CS. The test results are compared to the corresponding results for the untreated sand. The effects of CS concentration, curing time, density and confining stress on the monotonic and cyclic response of the treated sand are examined, among others. It is shown that stabilisation of the tested sand with CS significantly improves both the undrained monotonic and cyclic strength. The influence of CS concentration on the monotonic response is reduced with increasing mean effective stress. Cyclic resistance, however, is shown to be practically unaffected by CS concentration. Furthermore, cyclic straining at 5 to 10% of double-amplitude axial strain does not influence the undrained shear strength of the stabilised sand.
Performance of polymer-reinforced bituminous mixes using recycled coarse aggregate
Authors: Suresh Machavarapu , Pal Manish , Sarkar Dipankar
Published: November 2020

Bituminous mixes prepared using recycled coarse aggregate exhibit inferior characteristics compared to a control mix using natural stone aggregate. This is mostly due to the presence of loose cement mortar in the recycled aggregate. The study reported in this paper evaluated the performances of recycled aggregate mixes using different polymers, including centrifuged latex polymer, crumb rubber powder and polypropylene fibre. The performance of these polymer-reinforced mixes was then evaluated using a retained Marshall stability test, tensile strength ratio test, four-point bending test and wheel-tracking test. The results showed that all polymers improved the performance of the recycled aggregate mixes, making them better than the control mix. Moreover, cost analysis revealed that unmodified, centrifuged latex polymer and crumb rubber powder are more cost-effective than the control mix, whereas the polypropylene fibre mix was found to be expensive in terms of initial cost. Life-cycle cost analysis, however, showed that the fibre mix was also economical over the long run compared to the control mix. Overall, all polymer-modified mixes using recycled coarse aggregate showed better performance and were more cost-effective that natural stone aggregate mixes, meaning they are a viable alternative for road construction.
Alzheimer Hastalığıyla İlişkili Yaşam Kalitesi Ölçeği (AHİYK)’nin Türkçe Versiyonunun Geçerlilik Güvenilirlik Çalışması
Authors: BOZDEMİR Meral , KARŞIDAĞ Sibel , SAHİN Şevki , ÇINAR Nilgün
Published: November 2020

Amaç: Çalışmada Alzheimer Hastalığı ile İlişkili Yaşam Kalitesi (AHİYK) Ölçeği – (Alzheimer’s Disease Related Quality of Life (ADRQL) Scale)’nin psikometrik özellikleri ve AHİYK ölçeği puanı ile bakım verenlerin duygu durumları arasındaki ilişki incelenmiştir. Gereç ve Yöntem: Çalışmanın örneklemini, evde (N=30) ve bakım evinde (N=30) bakım gören toplam 60 muhtemel AH hastasının bakım vereni oluşturmaktadır. Klinik değerlendirme ölçeğine göre evre 1 ve 2 AH hastaları çalışma kapsamına alınmıştır. Hastaların bilişsel durumları Standardize Mini-Mental Test (SMMT) ile değerlendirilmiştir. Fonksiyonel durumu AH işbirliği çalışması - günlük yaşam aktiviteleri (ADCS-ADL) ölçeği ile değerlendirilmiştir. Bakım verenlerin duygu durumlarını değerlendirmek için Pozitif ve Negatif Duygu Ölçeği (PANAS) uygulanmıştır. Bu parametreler, bakım veren tarafından doldurulan AHİYK ölçeği ve 5 alt boyutu ( A.Sosyal etkileşim, B. Benlik bilinci C: Duygulanım ve duygudurum, D. Etkinliklerden keyif alma, E. çevre ile etkileşim) ile karşılaştırılmıştır. Güvenirlilik açısından 3 hafta sonra AHİYK ölçeği bakım verenlere tekrar uygulanmıştır. Bulgular: AHİYK total puanı ve B alt boyutu ile ADCS-ADL ölçeği arasında anlamlı düzeyde bir korelasyon saptanmıştır (sırasıyla r=0.27, r=0.38 p≤0.05). SMMT ile AHİYK ölçeğinin toplam puanı, A, B, C alt boyutları arasında anlamlı düzeyde korelasyon saptanmıştır (sırasıyla AHİYK total puan r=0.72, A boyutu r=0.83, B boyutu r=0.47, C boyutu r= 0.43, p≤0.05). AHİYK ölçeğininnin iç tutarlılık güvenirliği için Cronbach alfa katsayısı (α) ( ( α = 0.75) hesaplanmıştır. Ölçeğin tekrarlanan test güvenirlilik sonuçlarına göre AHİYK birinci ölçüm ile ikinci ölçümün gerek toplam puanları gerek alt boyutlarının puanları, anlamlı düzeyde korelasyona sahiptir. Sonuç: Bulgular AHİYK’nın Türkçe versiyonunun geçerli ve güvenilir bir ölçme aracı olduğunu göstermektedir.
Immunomodulatory Activity in vitro and in vivo of Polysaccharides from Kabuli Chickpea (Cicer arietinum L.) Hull
Authors: Akhtar Hafiz Muhammad Saleem , Ye Zipeng , Abdin Mohamed , Hamed Yahya Saud , Chen Guijie , Zeng Xiaoxiong
Published: November 2020

Research background. Polysaccharides isolated from plants, fungi and bacteria are associated with immunomodulatory effects. Chickpea hull, which is regarded as food industrial waste, contains considerable amounts of antioxidants and bioactive compounds.

Experimental approach. In the present study, we investigated the immunomodulatory activity of polysaccharides from kabuli chickpea (Cicer arietinum L.) hull (CHPS). In vitro study was conducted with RAW264.7 cell line while in vivo study was carried out using specific pathogen-free BALB/c mouse animal model.

Results and conclusions. In in vitro test with RAW264.7 murine macrophage cells, the three purified fractions of chickpea hull polysaccharides showed potent immunomodulatory activity. Sample CHPS-3 showed stronger effect on cell viability, promoted the phagocytosis index to a greater extent and had the best effect on acid phosphatase activity. Moreover, it was found that CHPS-3 significantly (p<0.05) enhanced the secretion of nitrogen monoxide and cytokine (interleukins IL-6, IL-1β and tumour necrosis factor-alpha (TNF-α)) levels. In in vivo study, CHPS-3 improved thymus and spleen indices in cyclophosphamide-induced immunodeficient mice. Increased activities of lysozyme, catalase, superoxide dismutase and glutathione peroxidase, serum haemolysin content and total antioxidant capacity were observed, while the amount of malondialdehyde in the liver decreased.

Novelty and scientific contribution. The results suggest that chickpea hull polysaccharides enhanced the immune activity and could be developed as the ingredient of functional foods.