bluuebunny/crossref_metadata_2025 huggingface dataset:
Regulation of Gene Expression of Methionine Sulfoxide Reductases and Their New Putative Roles in Plants
Authors: Kalemba Ewa M. , Stolarska Ewelina
Published: March 2019

Oxidation of methionine to methionine sulfoxide is a type of posttranslational modification reversed by methionine sulfoxide reductases (Msrs), which present an exceptionally high number of gene copies in plants. The side-form general antioxidant function-specific role of each Msr isoform has not been fully studied. Thirty homologous genes of Msr type A (MsrA) and type B (MsrB) that originate from the genomes of Arabidopsis thaliana, Populus trichocarpa, and Oryza sativa were analyzed in silico. From 109 to 201 transcription factors and responsive elements were predicted for each gene. Among the species, 220 and 190 common transcription factors and responsive elements were detected for the MsrA and MsrB isoforms, respectively. In a comparison of 14 MsrA and 16 MsrB genes, 424 transcription factors and responsive elements were reported in both types of genes, with almost ten times fewer unique elements. The transcription factors mainly comprised plant growth and development regulators, transcription factors important in stress responses with significant overrepresentation of the myeloblastosis viral oncogene homolog (MYB) and no apical meristem, Arabidopsis transcription activation factor and cup-shaped cotyledon (NAC) families and responsive elements sensitive to ethylene, jasmonate, sugar, and prolamine. Gene Ontology term-based functional classification revealed that cellular, metabolic, and developmental process terms and the response to stimulus term dominated in the biological process category. Available experimental transcriptomic and proteomic data, in combination with a set of predictions, gave coherent results validating this research. Thus, new manners Msr gene expression regulation, as well as new putative roles of Msrs, are proposed.
Disease Affects Bdnf Expression in Synaptic and Extrasynaptic Regions of Skeletal Muscle of Three SBMA Mouse Models
Authors: Halievski Katherine , Nath Samir R. , Katsuno Masahisa , Adachi Hiroaki , Sobue Gen , Breedlove S. Marc , Lieberman Andrew P. , Jordan Cynthia L.
Published: March 2019

Spinal bulbar muscular atrophy (SBMA) is a slowly progressive, androgen-dependent neuromuscular disease in men that is characterized by both muscle and synaptic dysfunction. Because gene expression in muscle is heterogeneous, with synaptic myonuclei expressing genes that regulate synaptic function and extrasynaptic myonuclei expressing genes to regulate contractile function, we used quantitative PCR to compare gene expression in these two domains of muscle from three different mouse models of SBMA: the “97Q” model that ubiquitously expresses mutant human androgen receptor (AR), the 113Q knock-in (KI) model that expresses humanized mouse AR with an expanded glutamine tract, and the “myogenic” model that overexpresses wild-type rat AR only in skeletal muscle. We were particularly interested in neurotrophic factors because of their role in maintaining neuromuscular function via effects on both muscle and synaptic function, and their implicated role in SBMA. We confirmed previous reports of the enriched expression of select genes (e.g., the acetylcholine receptor) in the synaptic region of muscle, and are the first to report the synaptic enrichment of others (e.g., glial cell line-derived neurotrophic factor). Interestingly, all three models displayed comparably dysregulated expression of most genes examined in both the synaptic and extrasynaptic domains of muscle, with only modest differences between regions and models. These findings of comprehensive gene dysregulation in muscle support the emerging view that skeletal muscle may be a prime therapeutic target for restoring function of both muscles and motoneurons in SBMA.
Confinement Effect of Micro- and Mesoporous Materials on the Spectroscopy and Dynamics of a Stilbene Derivative Dye
Authors: di Nunzio Maria Rosaria , Perenlei Ganchimeg , Douhal Abderrazzak
Published: March 2019

Micro- and mesoporous silica-based materials are a class of porous supports that can encapsulate different guest molecules. The formation of these hybrid complexes can be associated with significant alteration of the physico-chemical properties of the guests. Here, we report on a photodynamical study of a push–pull molecule, trans-4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM), entrapped within faujasite-type zeolites (HY, NaX, and NaY) and MCM-41 in dichloromethane suspensions. The complex formation gives rise to caged monomers and H- and J-aggregates. Steady-state experiments show that the nanoconfinement provokes net blue shifts of both the absorption and emission spectra, which arise from preferential formation of H-aggregates concomitant with a distortion and/or protonation of the DCM structure. The photodynamics of the hybrid complexes are investigated by nano- to picosecond time-resolved emission experiments. The obtained fluorescence lifetimes are 65–99 ps and 350–400 ps for H- and J-aggregates, respectively, while those of monomers are 2.46–3.87 ns. Evidences for the presence of a charge-transfer (CT) process in trapped DCM molecules (monomers and/or aggregates) are observed. The obtained results are of interest in the interpretation of electron-transfer processes, twisting motions of analogues push–pull systems in confined media and understanding photocatalytic mechanisms using this type of host materials.
Molecular Mechanisms of Hypothalamic Insulin Resistance
Authors: Ono Hiraku
Published: March 2019

Insulin exists in the central nervous system, where it executes two important functions in the hypothalamus: the suppression of food intake and the improvement of glucose metabolism. Recent studies have shown that both are exerted robustly in rodents and humans. If intact, these functions exert beneficial effects on obesity and diabetes, respectively. Disruption of both occurs due to a condition known as hypothalamic insulin resistance, which is caused by obesity and the overconsumption of saturated fat. An enormous volume of literature addresses the molecular mechanisms of hypothalamic insulin resistance. IKKβ and JNK are major players in the inflammation pathway, which is activated by saturated fatty acids that induce hypothalamic insulin resistance. Two major tyrosine phosphatases, PTP-1B and TCPTP, are upregulated in chronic overeating. They dephosphorylate the insulin receptor and insulin receptor substrate proteins, resulting in hypothalamic insulin resistance. Prolonged hyperinsulinemia with excessive nutrition activates the mTOR/S6 kinase pathway, thereby enhancing IRS-1 serine phosphorylation to induce hypothalamic insulin resistance. Other mechanisms associated with this condition include hypothalamic gliosis and disturbed insulin transport into the central nervous system. Unveiling the precise molecular mechanisms involved in hypothalamic insulin resistance is important for developing new ways of treating obesity and type 2 diabetes.
Intranasal Borna Disease Virus (BoDV-1) Infection: Insights into Initial Steps and Potential Contagiosity
Authors: Kupke Alexandra , Becker Sabrina , Wewetzer Konstantin , Ahlemeyer Barbara , Eickmann Markus , Herden Christiane
Published: March 2019

Mammalian Bornavirus (BoDV-1) typically causes a fatal neurologic disorder in horses and sheep, and was recently shown to cause fatal encephalitis in humans with and without transplant reception. It has been suggested that BoDV-1 enters the central nervous system (CNS) via the olfactory pathway. However, (I) susceptible cell types that replicate the virus for successful spread, and (II) the role of olfactory ensheathing cells (OECs), remained unclear. To address this, we studied the intranasal infection of adult rats with BoDV-1 in vivo and in vitro, using olfactory mucosal (OM) cell cultures and the cultures of purified OECs. Strikingly, in vitro and in vivo, viral antigen and mRNA were present from four days post infection (dpi) onwards in the olfactory receptor neurons (ORNs), but also in all other cell types of the OM, and constantly in the OECs. In contrast, in vivo, BoDV-1 genomic RNA was only detectable in adult and juvenile ORNs, nerve fibers, and in OECs from 7 dpi on. In vitro, the rate of infection of OECs was significantly higher than that of the OM cells, pointing to a crucial role of OECs for infection via the olfactory pathway. Thus, this study provides important insights into the transmission of neurotropic viral infections with a zoonotic potential.
Arctigenin Reduces Myofibroblast Activities in Oral Submucous Fibrosis by LINC00974 Inhibition
Authors: Lin Ching-Yeh , Hsieh Pei-Ling , Liao Yi-Wen , Peng Chih-Yu , Yu Cheng-Chia , Lu Ming-Yi
Published: March 2019

Oral submucous fibrosis (OSF) is an oral precancerous condition associated with the habit of areca nut chewing and the TGF-β pathway. Currently, there is no curative treatment to completely heal OSF, and it is imperative to alleviate patients’ symptoms and prevent it from undergoing malignant transformation. Arctigenin, a lignan extracted from Arctium lappa, has been reported to have a variety of pharmacological activities, including anti-fibrosis. In the present study, we examined the effect of arctigenin on the cell proliferation of buccal mucosal fibroblasts (BMFs) and fibrotic BMFs (fBMFs), followed by assessment of myofibroblast activities. We found that arctigenin was able to abolish the arecoline-induced collagen gel contractility, migration, invasion, and wound healing capacities of BMFs and downregulate the myofibroblast characteristics of fBMFs in a dose-dependent manner. Most importantly, the production of TGF-β in fBMFs was reduced after exposure to arctigenin, along with the suppression of p-Smad2, α-smooth muscle actin, and type I collagen A1. In addition, arctigenin was shown to diminish the expression of LINC00974, which has been proven to activate TGF-β/Smad signaling for oral fibrogenesis. Taken together, we demonstrated that arctigenin may act as a suitable adjunct therapy for OSF.
Transcriptomic Analysis of Porcine Endometrium during Implantation after In Vitro Stimulation by Adiponectin
Authors: Smolinska Nina , Szeszko Karol , Dobrzyn Kamil , Kiezun Marta , Rytelewska Edyta , Kisielewska Katarzyna , Gudelska Marlena , Bors Kinga , Wyrebek Joanna , Kopij Grzegorz , Kaminska Barbara , Kaminski Tadeusz
Published: March 2019

Comprehensive understanding of the regulatory mechanism of the implantation process in pigs is crucial for reproductive success. The endometrium plays an important role in regulating the establishment and maintenance of gestation. The goal of the current study was to determine the effect of adiponectin on the global expression pattern of genes and relationships among differentially expressed genes (DE-genes) in the porcine endometrium during implantation using microarrays. Diverse transcriptome analyses including gene ontology (GO), biological pathway, networks, and DE-gene analyses were performed. Adiponectin altered the expression of 1286 genes with fold-change (FC) values greater than 1.2 (p < 0.05). The expression of 560 genes were upregulated and 726 downregulated in the endometrium treated with adiponectin. Thirteen genes were selected for real-time PCR validation of differential expression based on a known role in metabolism, steroid and prostaglandin synthesis, interleukin and growth factor action, and embryo implantation. Functional analysis of the relationship between DE-genes indicated that adiponectin interacts with genes that are involved in the processes of cell proliferation, programmed cell death, steroid and prostaglandin synthesis/metabolism, cytokine production, and cell adhesion that are critical for reproductive success. The presented results suggest that adiponectin signalling may play a key role in the implantation of pig.
Primary Cilium in Cancer Hallmarks
Authors: Fabbri Lucilla , Bost Frédéric , Mazure Nathalie M.
Published: March 2019

The primary cilium is a solitary, nonmotile and transitory appendage that is present in virtually all mammalian cells. Our knowledge of its ultrastructure and function is the result of more than fifty years of research that has dramatically changed our perspectives on the primary cilium. The mutual regulation between ciliogenesis and the cell cycle is now well-recognized, as well as the function of the primary cilium as a cellular “antenna” for perceiving external stimuli, such as light, odorants, and fluids. By displaying receptors and signaling molecules, the primary cilium is also a key coordinator of signaling pathways that converts extracellular cues into cellular responses. Given its critical tasks, any defects in primary cilium formation or function lead to a wide spectrum of diseases collectively called “ciliopathies”. An emerging role of primary cilium is in the regulation of cancer development. In this review, we seek to describe the current knowledge about the influence of the primary cilium in cancer progression, with a focus on some of the events that cancers need to face to sustain survival and growth in hypoxic microenvironment: the cancer hallmarks.
Plant Serine Protease Inhibitors: Biotechnology Application in Agriculture and Molecular Farming
Authors: Clemente Marina , Corigliano Mariana G. , Pariani Sebastián A. , Sánchez-López Edwin F. , Sander Valeria A. , Ramos-Duarte Víctor A.
Published: March 2019

The serine protease inhibitors (SPIs) are widely distributed in living organisms like bacteria, fungi, plants, and humans. The main function of SPIs as protease enzymes is to regulate the proteolytic activity. In plants, most of the studies of SPIs have been focused on their physiological role. The initial studies carried out in plants showed that SPIs participate in the regulation of endogenous proteolytic processes, as the regulation of proteases in seeds. Besides, it was observed that SPIs also participate in the regulation of cell death during plant development and senescence. On the other hand, plant SPIs have an important role in plant defense against pests and phytopathogenic microorganisms. In the last 20 years, several transgenic plants over-expressing SPIs have been produced and tested in order to achieve the increase of the resistance against pathogenic insects. Finally, in molecular farming, SPIs have been employed to minimize the proteolysis of recombinant proteins expressed in plants. The present review discusses the potential biotechnological applications of plant SPIs in the agriculture field.
The Role of Tocotrienol in Preventing Male Osteoporosis—A Review of Current Evidence
Authors: Chin Kok-Yong , Ima-Nirwana Soelaiman
Published: March 2019

Male osteoporosis is a significant but undetermined healthcare problem. Men suffer from a higher mortality rate post-fracture than women and they are marginalized in osteoporosis treatment. The current prophylactic agents for osteoporosis are limited. Functional food components such as tocotrienol may be an alternative option for osteoporosis prevention in men. This paper aims to review the current evidence regarding the skeletal effects of tocotrienol in animal models of male osteoporosis and its potential antiosteoporotic mechanism. The efficacy of tocotrienol of various sources (single isoform, palm and annatto vitamin E mixture) had been tested in animal models of bone loss induced by testosterone deficiency (orchidectomy and buserelin), metabolic syndrome, nicotine, alcoholism, and glucocorticoid. The treated animals showed improvements ranging from bone microstructural indices, histomorphometric indices, calcium content, and mechanical strength. The bone-sparing effects of tocotrienol may be exerted through its antioxidant, anti-inflammatory, and mevalonate-suppressive pathways. However, information pertaining to its mechanism of actions is superficial and warrants further studies. As a conclusion, tocotrienol could serve as a functional food component to prevent male osteoporosis, but its application requires validation from a clinical trial in men.
Functional Interaction among KCa and TRP Channels for Cardiovascular Physiology: Modern Perspectives on Aging and Chronic Disease
Authors: Behringer Erik J. , Hakim Md A.
Published: March 2019

Effective delivery of oxygen and essential nutrients to vital organs and tissues throughout the body requires adequate blood flow supplied through resistance vessels. The intimate relationship between intracellular calcium ([Ca2+]i) and regulation of membrane potential (Vm) is indispensable for maintaining blood flow regulation. In particular, Ca2+-activated K+ (KCa) channels were ascertained as transducers of elevated [Ca2+]i signals into hyperpolarization of Vm as a pathway for decreasing vascular resistance, thereby enhancing blood flow. Recent evidence also supports the reverse role for KCa channels, in which they facilitate Ca2+ influx into the cell interior through open non-selective cation (e.g., transient receptor potential; TRP) channels in accord with robust electrical (hyperpolarization) and concentration (~20,000-fold) transmembrane gradients for Ca2+. Such an arrangement supports a feed-forward activation of Vm hyperpolarization while potentially boosting production of nitric oxide. Furthermore, in vascular types expressing TRP channels but deficient in functional KCa channels (e.g., collecting lymphatic endothelium), there are profound alterations such as downstream depolarizing ionic fluxes and the absence of dynamic hyperpolarizing events. Altogether, this review is a refined set of evidence-based perspectives focused on the role of the endothelial KCa and TRP channels throughout multiple experimental animal models and vascular types. We discuss the diverse interactions among KCa and TRP channels to integrate Ca2+, oxidative, and electrical signaling in the context of cardiovascular physiology and pathology. Building from a foundation of cellular biophysical data throughout a wide and diverse compilation of significant discoveries, a translational narrative is provided for readers toward the treatment and prevention of chronic, age-related cardiovascular disease.
Tracking and Linking of Microparticle Trajectories During Mode-Coupling Induced Melting in a Two-Dimensional Complex Plasma Crystal
Authors: Couëdel Lénaïc , Nosenko Vladimir
Published: March 2019

In this article, a strategy to track microparticles and link their trajectories adapted to the study of the melting of a quasi two-dimensional complex plasma crystal induced by the mode-coupling instability is presented. Because of the three-dimensional nature of the microparticle motions and the inhomogeneities of the illuminating laser light sheet, the scattered light intensity can change significantly between two frames, making the detection of the microparticles and the linking of their trajectories quite challenging. Thanks to a two-pass noise removal process based on Gaussian blurring of the original frames using two different kernel widths, the signal-to-noise ratio was increased to a level that allowed a better intensity thresholding of different regions of the images and, therefore, the tracking of the poorly illuminated microparticles. Then, by predicting the positions of the microparticles based on their previous positions, long particle trajectories could be reconstructed, allowing accurate measurement of the evolution of the microparticle energies and the evolution of the monolayer properties.
SCR Performance Evaluations in Relation to Experimental Parameters in a Marine Generator Engine
Authors: Kim Dongkyu , Lee Changhee
Published: March 2019

Many researchers have conducted experiments on the position and nozzle conditions in the exhaust pipe of a stage/swirl mixer, as well as the injection position required to satisfy the International Maritime Organisation (IMO) regulations. In this study, an SCR (Selective catalytic reduction) system was designed through basic and detailed designs. The performance of the components was verified through a single-component performance test for the detailed design components, and the total nitrogen oxide (NOx) reduction efficiency was predicted using the proven system. Subsequently, the system was applied to an actual engine, and the performance was verified according to the engine operating conditions using the experimental variables. The NOx reduction rate and NH3 slip characteristics in relation to MCR (Maximum Continuous Rating) conditions using the optimised SCR system indicate that the NOx reduction rate exceeds 80% in all MCR conditions. Finally, the SCR system developed in this study proves that the performance of the SCR system satisfies the IMO regulatory requirements.
Generalized (ψ,α,β)—Weak Contractions for Initial Value Problems
Authors: Borisut Piyachat , Kumam Poom , Gupta Vishal , Mani Naveen
Published: March 2019

A class of generalized ( ψ , α , β ) —weak contraction is introduced and some fixed-point theorems in a framework of partially ordered metric spaces are proved. The main result of this paper is applied to a first-order ordinary differential equation to find its solution.
On Irregularity Measures of Some Dendrimers Structures
Authors: Gao Wei , Aamir Muhammad , Iqbal Zahid , Ishaq Muhammad , Aslam Adnan
Published: March 2019

A graph is said to be a regular graph if all its vertices have the same degree, otherwise, it is irregular. Irregularity indices are usually used for quantitative characterization of the topological structure of non-regular graphs. In numerous applications and problems in material engineering and chemistry, it is useful to be aware that how irregular a molecular structure is? Furthermore, evaluations of the irregularity of underline molecular graphs could be valuable for QSAR/QSPR studies, and for the expressive determines of chemical and physical properties, such as enthalpy of vaporization, toxicity, resistance, Entropy, melting and boiling points. In this paper, we think over the following four irregularity measures: the irregularity index by Albertson, σ irregularity index, the total irregularity index and the variance of vertex degrees. By way of graph structural estimation and derivations, we determine these irregularity measures of the molecular graphs of different classes of dendrimers.
Adaptive Makeup Transfer via Bat Algorithm
Authors: Ren Yeqing , Sun Youqiang , Jing Xuechun , Cui Zhihua , Shi Zhentao
Published: March 2019

With the advent of the artificial intelligence (AI) era, the beauty camera is widely used, and makeup transfer has attracted increasing attention. In this paper, we propose an adaptive makeup transfer based on the bat algorithm to solve the problem that only a single makeup effect can be transferred. According to the characteristics of makeup style, the algorithm optimizes the weight value to get the appropriate makeup lightness by using the adaptive method. The improved algorithm can not only help to get the optimal weight values in the process of transferring the same makeup style to different targets, but also to transfer different makeup styles to the same target. Moreover, this algorithm can choose the most suitable makeup style and also the most appropriate lightness for a certain person. Experimental results show that the algorithm proposed in the paper has a better effect than the existing algorithm of makeup transfer, and the algorithm can provide users with a suitable makeup style and appropriate lightness.
Preparation and Evaluation of Peptides with Potential Antioxidant Activity by Microwave Assisted Enzymatic Hydrolysis of Collagen from Sea Cucumber Acaudina Molpadioides Obtained from Zhejiang Province in China
Authors: Jin Huo-Xi , Xu Hong-Ping , Li Yan , Zhang Qian-Wei , Xie Hui
Published: March 2019

The present study was focused on the preparation and characterization of the antioxidant peptides by microwave-assisted enzymatic hydrolysis of collagen from sea cucumber Acaudina molpadioides (ASC-Am) obtained from Zhejiang Province in China. The results exhibited the effects of microwave irradiation on hydrolysis of ASC-Am with different protease. Neutrase was selected from the four common proteases (papain, pepsin, trypsin, and neutrase) based on the highest content and DPPH scavenging activity of hydrolysate Fa (Molecular weight < 1 kDa). The content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of Fa obtained by hydrolysis of neutrase increased by 100% and 109% respectively at a microwave power of 300 W compared with no microwave irradiation. Five subfractions were obtained after performing the gel filtration chromatography, and the Fa.2 exhibited the highest DPPH scavenging activity. The amino acid analysis showed that the contents of Glutamic acid, Alanine, Tyrosine, and Phenylalanine in fraction Fa.2 increased significantly, but an obvious decrease in the content of Glycine was observed compared to Fa. Four peptides (Fa.2-A, Fa.2-B, Fa.2-C, and Fa.2-D) were purified from Fa.2 by high performance liquid chromatography, and Fa.2-C showed the highest DPPH scavenging activity. The sequence of Fa.2-C was identified as Phenylalanine-Leucine- Alanine-Proline with a half elimination ratio (EC50) of 0.385 mg/mL. The antioxidant activity of Fa.2-C was probably attributed to the small molecular sizes and the presence of hydrophobic amino acid residues in its sequence. This report provided a promising method for the preparation of antioxidant peptides from collagen for food and medicinal purposes.
In Silico Analysis of the Subtype Selective Blockage of KCNA Ion Channels through the µ-Conotoxins PIIIA, SIIIA, and GIIIA
Authors: Kaufmann Desirée , Tietze Alesia A. , Tietze Daniel
Published: March 2019

Understanding subtype specific ion channel pore blockage by natural peptide-based toxins is crucial for developing such compounds into promising drug candidates. Herein, docking and molecular dynamics simulations were employed in order to understand the dynamics and binding states of the µ-conotoxins, PIIIA, SIIIA, and GIIIA, at the voltage-gated potassium channels of the KV1 family, and they were correlated with their experimental activities recently reported by Leipold et al. Their different activities can only adequately be understood when dynamic information about the toxin-channel systems is available. For all of the channel-bound toxins investigated herein, a certain conformational flexibility was observed during the molecular dynamic simulations, which corresponds to their bioactivity. Our data suggest a similar binding mode of µ-PIIIA at KV1.6 and KV1.1, in which a plethora of hydrogen bonds are formed by the Arg and Lys residues within the α-helical core region of µ-PIIIA, with the central pore residues of the channel. Furthermore, the contribution of the K+ channel’s outer and inner pore loops with respect to the toxin binding. and how the subtype specificity is induced, were proposed.
Neurorehabilitation of Traumatic Brain Injury (TBI): A Clinical Review
Authors: Oberholzer Michael , Müri René M.
Published: March 2019

Traumatic brain injury (TBI) and its potential long-term consequences are of major concern for public health. Neurorehabilitation of affected individuals has some specific characteristics in contrast to neurorehabilitation of patients with acquired brain lesions of other aetiology. This review will deal with the clinical consequences of the distinct lesions of TBI. In severe TBI, clinical course often follows a typical initial sequence of coma; followed by disturbed consciousness; later, post-traumatic agitation and amnesia; and finally, recovery of function occurs. In the different phases of neurorehabilitation, physicians should be aware of typical medical complications such as paroxysmal sympathetic hyperactivity, posttraumatic hydrocephalus, and posttraumatic neuroendocrine dysfunctions. Furthermore, we address questions on timing and on existing evidence for different rehabilitation programmes and for holistic neuropsychological rehabilitation approaches.
Generational Health Impact of PCOS on Women and Their Children
Authors: Hart Roger
Published: March 2019

Polycystic ovary syndrome (PCOS) is a metabolic disorder with reproductive consequences. Hence, the synergy of the dual maternal challenges of difficulties with conception, set on a background of metabolic disorder and inflammation, understandably leads to increased obstetric risk for the woman. Furthermore, she is more likely than her peers to require assistance with conception, either through induction of ovulation with the attendant risk of a multiple gestation, or in vitro fertilization (IVF) with its recognized increased obstetric risk for woman and her child. The increased obstetric risk for a woman with PCOS is manifested with an increased rate of miscarriage, gestational diabetes, hypertensive disorder and premature delivery. These obstetric complications are due to impairment of placental function, systemic inflammation and metabolic disorder and are markers for the woman herself of her predisposition to cardiometabolic disorder in later life. Consequently, it is inevitable that this environment may induce changes in the fetus during pregnancy, leading to an intergenerational risk from maternal PCOS.
Correction: Kutlushina, A., et al. Ligand-Based Pharmacophore Modeling Using Novel 3D Pharmacophore Signatures. Molecules, 2018, 23, 3094
Authors: Kutlushina Alina , Khakimova Aigul , Madzhidov Timur , Polishchuk Pavel
Published: March 2019

The authors would like to add the funding number to the published article [...]
GC-MS Analysis of the Composition of the Extracts and Essential Oil from Myristica fragrans Seeds Using Magnesium Aluminometasilicate as Excipient
Authors: Matulyte Inga , Marksa Mindaugas , Ivanauskas Liudas , Kalvėnienė Zenona , Lazauskas Robertas , Bernatoniene Jurga
Published: March 2019

Myristica fragrans (f. Myristicaceae) seeds are better known as a spice, but their chemical compounds may have a pharmacological effect. The yield of their composition of extracts and essential oils differs due to different methodologies. The aim of this study was to evaluate an excipient material—magnesium aluminometasilicate—and to determine its influence on the qualitative composition of nutmeg extracts and essential oils. Furthermore, we wanted to compare the yield of essential oil. The extracts were prepared by maceration (M) and ultrasound bath-assisted extraction (UAE), and the essential oil—by hydrodistillation (HD). Conventional methods (UAE, HD) were modified with magnesium aluminometasilicate. The samples were analyzed by gas chromatography-mass spectrometry (GC-MS) method. From 16 to 19 chemical compounds were obtained using UAE with magnesium aluminometasilicate, while only 8 to 13 compounds were obtained using UAE without an excipient. Using our conditions and plant material, for the first time eight new chemical compounds in nutmeg essential oil were identified. Two of these compounds (γ-amorphene and cis-α-bergamotene) were obtained with the use of excipient, the other six (β-copaene, bergamotene, citronellyl decanoate, cubebol, cubenene, orthodene) by conventional hydrodistillation. Magnesium aluminometasilicate significantly increased the quantity of sabinene (from 6.53% to 61.42%) and limonene (from 0% to 5.62%) in essential oil. The yield of the essential oil from nutmeg seeds was significantly higher using magnesium aluminometasilicate; it increased from 5.25 ± 0.04% to 10.43 ± 0.09%.
Light-Induced Control of the Spin Distribution on Cu–Dithiolene Complexes: A Correlated Ab Initio Study
Authors: Zapata-Rivera Jhon , Calzado Carmen J.
Published: March 2019

Metal dithiolene complexes—M(dmit)2—are key building blocks for magnetic, conducting, and optical molecular materials, with singular electronic structures resulting from the mixing of the metal and dmit ligand orbitals. Their use in the design of magnetic and conducting materials is linked to the control of the unpaired electrons and their localized/delocalized nature. It has been recently found that UV–Vis light can control the spin distribution of some [Cu(dmit)2]−2 salts in a direct and reversible way. In this work, we study the optical response of these salts and the origin of the differences observed in the EPR spectra under UV–Vis irradiation by means of wave function-based quantum chemistry methods. The low-lying states of the complex have been characterized and the electronic transitions with a non-negligible oscillator strength have been identified. The population of the corresponding excited states promoted by the UV–Vis absorption produces significant changes in the spin distribution, and could explain the changes observed in the system upon illumination. The interaction between neighbor [Cu(dmit)2]−2 complexes is weakly ferromagnetic, consistent with the relative orientation of the magnetic orbitals and the crystal packing, but in disagreement with previous assignments. Our results put in evidence the complex electronic structure of the [Cu(dmit)2]−2 radical and the relevance of a multideterminantal approach for an adequate analysis of their properties.
DEM Investigation of the Influence of Minerals on Crack Patterns and Mechanical Properties of Red Mudstone
Authors: Zhang Shuai , Zhang Dongsheng , Zhao Qiang , Chi Mingbo , Zhang Wei , Yu Wei
Published: March 2019

Rocks are natural heterogeneous materials. It is common for a rock to have several kinds of minerals, which will have a significant effect on its mechanical behavior. The purpose of the numerical simulation study in this paper is to explore the effects of minerals on the crack patterns and mechanical properties of rocks. First, the corresponding calculation model is established by using the discrete element method (DEM), whereby the mechanical parameters of the blocks and joints in the Tyson polygon procedure are fitted with the rock properties obtained in the laboratory. Then, various combination models of different mineral sizes and ratios are established to study the effects of mineral size, position, and ratio on the fracture distribution and mechanical properties of rock samples. The results indicate that with increased circle size of the center mineral and the mineral ratio, the elastic modulus and uniaxial compression strength (UCS) of the model gradually increase. The drop degree of post-peak stress decreases, and the integrity and bearing capacity increase. It is found that there is a quartic polynomial relationship between elastic modulus and mineral circle radius, with R2 ≥ 0.94. The minerals located in the crack propagation path will effectively block the crack and change the propagation direction. When the mineral position is close to the model boundaries, especially the lateral boundaries, it has a significant influence on the crack initiation position, causing crack initiation to occur at the upper boundary of the mineral circle and propagate to the middle of the model. With increased mineral percentage and small-size mineral circle proportion, the depth of extension of the crack from boundary to center is reduced, the crack has wide development in the boundary area, the number of central cracks in the rock specimen decreases, and the degree of fragmentation decreases.
A Novel PZT Pump with Built-in Compliant Structures
Authors: Bao Qibo , Zhang Jianhui , Tang Ming , Huang Zhi , Lai Liyi , Huang Jun , Wu Chuanyu
Published: March 2019

Different to the traditionally defined valved piezoelectric (PZT) pump and valveless PZT pump, two groups of PZT pumps with built-in compliant structures—with distances between the free ends of 0.2 mm (Group A) and 0 mm (Group B)—were designed, fabricated, and experimentally tested. This type of pump mainly contains a chamber 12 mm in diameter and 1.1 mm in height, a PZT vibrator, and two pairs of compliant structures arranged on the flowing channel. The flow-resistance differences between these two groups of PZT pumps were theoretically and experimentally verified. The relationships between the amplitude, applied voltage and frequency of the PZT vibrators were obtained experimentally, with results illustrating that the amplitude linearly and positively correlates with the voltage, while nonlinearly and negatively correlating to the frequency. The flow rate performance of these two groups was experimentally tested from 110–160 Vpp and 10–130 Hz. Results showed that the flow rate positively correlates to the voltage, and the optimum flow rate frequency centers around 90 Hz for Group A and 80 Hz for Group B, respectively. The flow rate performances of Group B were further measured from 60–100 Hz and 170–210 Vpp, and obtained optimal flow rates of 3.6 mL/min at 210 Vpp and 80 Hz when ignoring the siphon-caused backward flow rate. As the compliant structures are not prominently limited by the channel’s size, and the pump can be minimized by Micro-electromechanical Systems (MEMS) processing methods, it is a suitable candidate for microfluidic applications like closed-loop cooling systems and drug delivery systems.
An AutomationML Based Ontology for Sensor Fusion in Industrial Plants
Authors: Gonçalves Eder Mateus Nunes , Freitas Alvaro , Botelho Silvia
Published: March 2019

AutomationML (AML) can be seen as a partial knowledge-based solution for manufacturing and automation domains since it permits integrating different engineering data format, and also contains information about physical and logical structures of production systems, using basic concepts as resources, process, and products, in semantic structures. However, it is not a complete knowledge-based solution because it does not have mechanisms for querying and reasoning procedures, which are basic functions for semantic inferences. Additionally, AutomationML does not deal with aspects of sensor fusion naturally. In this sense, we propose an ontology to describe those sensors’ fusion elements, including procedures for runtime processing, and also elements that can turn AutomationML into a complete knowledge-based solution. The approach was applied in a case study with two different industrial processes with some sensors under fusion. The results obtained demonstrate that the ontology allows describing sensors that are under fusion and deal with the occurrence of data divergence. In a broader view, the results show how to apply AutomationML description for runtime processing of data generated from different sensors of a manufacturing system using an ontology to complement the AML description, where AutomationML concentrates knowledge about a specific production system and the ontology describes a general and reusable knowledge about sensor fusion.
A Convenient Calibration Method for LRF-Camera Combination Systems Based on a Checkerboard
Authors: Zhang Zhuang , Zhao Rujin , Liu Enhai , Yan Kun , Ma Yuebo
Published: March 2019

In this paper, a simple and easy high-precision calibration method is proposed for the LRF-camera combined measurement system which is widely used at present. This method can be applied not only to mainstream 2D and 3D LRF-cameras, but also to calibrate newly developed 1D LRF-camera combined systems. It only needs a calibration board to record at least three sets of data. First, the camera parameters and distortion coefficients are decoupled by the distortion center. Then, the spatial coordinates of laser spots are solved using line and plane constraints, and the estimation of LRF-camera extrinsic parameters is realized. In addition, we establish a cost function for optimizing the system. Finally, the calibration accuracy and characteristics of the method are analyzed through simulation experiments, and the validity of the method is verified through the calibration of a real system.
Stretchable Pressure Sensor with Leakage-Free Liquid-Metal Electrodes
Authors: Zhang Lunjia , Gao Meng , Wang Ronghang , Deng Zhongshan , Gui Lin
Published: March 2019

Nowadays, with the development of wearable devices, stretchable pressure sensors have been widely adopted in all kinds of areas. Most of the sensors aim to detect small pressure, such as fingertip tactile sensing, but only a few are focused on high-pressure sensing, such as foot pressure sensing during men’s walking. In this work, a liquid metal-based stretchable sensor for large-pressure measurement is investigated. This sensor is fully stretchable because it is made of soft materials. However, when the soft sensor is subjected to high pressure, the liquid metal easily leaks from microchannels because it maintains the liquid state at room temperature. We therefore propose to fabricate liquid metal-based leakage-free electrodes to handle the liquid-metal leak. Parametric studies are conducted to compare this sensor with liquid-metal-only electrodes and leakage-free electrodes. The leakage-free electrodes increase the measurement ranges from 0.18, 0.18, and 0.15 MPa to 0.44 MPa, with higher linearity and precision. The improvement in the liquid-metal electrode enables the sensors to work stably within 0.44 MPa pressure and 20% strain. In addition, we integrate two capacitors, namely, a working capacitor and a reference capacitor, into one sensor to reduce the influence of parasitic capacitance brought about by external interference. This stretchable capacitive sensor capable of working under a wide range of pressure with good repeatability, sensitivity, and linearity, exhibits great potential use for wearable electronics. Finally, the method for fabricating leakage-free electrodes shows great value for hyperelastic electronics manufacturing and micromachine technology.
A Multi-Feature and Multi-Level Matching Algorithm Using Aerial Image and AIS for Vessel Identification
Authors: Xiu Supu , Wen Yuanqiao , Yuan Haiwen , Xiao Changshi , Zhan Wenqiang , Zou Xiong , Zhou Chunhui , Shah Sayed Chhattan
Published: March 2019

In order to monitor and manage vessels in channels effectively, identification and tracking are very necessary. This work developed a maritime unmanned aerial vehicle (Mar-UAV) system equipped with a high-resolution camera and an Automatic Identification System (AIS). A multi-feature and multi-level matching algorithm using the spatiotemporal characteristics of aerial images and AIS information was proposed to detect and identify field vessels. Specifically, multi-feature information, including position, scale, heading, speed, etc., are used to match between real-time image and AIS message. Additionally, the matching algorithm is divided into two levels, point matching and trajectory matching, for the accurate identification of surface vessels. Through such a matching algorithm, the Mar-UAV system is able to automatically identify the vessel’s vision, which improves the autonomy of the UAV in maritime tasks. The multi-feature and multi-level matching algorithm has been employed for the developed Mar-UAV system, and some field experiments have been implemented in the Yangzi River. The results indicated that the proposed matching algorithm and the Mar-UAV system are very significant for achieving autonomous maritime supervision.
RF Energy Harvesting System Based on an Archimedean Spiral Antenna for Low-Power Sensor Applications
Authors: Alex-Amor Antonio , Palomares-Caballero Ángel , Fernández-González José M. , Padilla Pablo , Marcos David , Sierra-Castañer Manuel , Esteban Jaime
Published: March 2019

This paper presents a radiofrequency (RF) energy harvesting system based on an ultrawideband Archimedean spiral antenna and a half-wave Cockcroft-Walton multiplier circuit. The antenna was proved to operate from 350 MHz to 16 GHz with an outstanding performance. With its use, radio spectrum measurements were carried out at the Telecommunication Engineering School (Universidad Politécnica de Madrid) to determine the power level of the ambient signals in two different scenarios: indoors and outdoors. Based on these measurements, a Cockcroft-Walton multiplier and a lumped element matching network are designed to operate at 800 MHz and 900 MHz frequency bands. To correct the frequency displacement in the circuit, a circuit model is presented that takes into account the different parasitic elements of the components and the PCB. With an input power of 0 dBm, the manufactured circuit shows a rectifying efficiency of 30%. Finally, a test is carried out with the full RF energy harvesting system to check its correct operation. Thus, the RF system is placed in front of a transmitting Vivaldi antenna at a distance of 50 cm. The storage capacitor has a charge of over 1.25 V, which is enough to run a temperature sensor placed as the load to be supplied. This demonstrates the validity of the RF energy harvesting system for low-power practical applications.
Noise Suppression for Direction of Arrival Estimation in Co-located MIMO Sonar
Authors: Cheng Xue , Wang Yingmin
Published: March 2019

Noise suppression capacity in multiple-input multiple-output (MIMO) sonar signal processing is derived under the assumption of white Gaussian noise. However, underwater noise mainly includes white Gaussian noise and colored noise. There exists a certain correlation between the noise signals received by each MIMO sonar array element. The performance of traditional direction-of-arrival (DOA) estimation methods decreases obviously in complex marine noise. In this paper, we propose a marine environment noise suppression method for MIMO applied to multiple targets’ DOA estimation. The noise field can be decomposed into a symmetric noise component and an asymmetric noise component. We use the covariance matrix imaginary component to pre-estimate the signal sources, then use the dimension reduction transformation to reconstruct the real component of the covariance matrix. The Toeplitz technique is utilized to reduce the correlation of the reconstructed covariance matrix. Thus, the subspace decomposition-based techniques such as multiple signal classification (MUSIC) can be used for multiple targets’ DOA estimation. To reduce the computational complexity of the methods, search-free direction-finding techniques such as the estimation of signal parameters via rotational invariance techniques (ESPRIT) can be utilized. As a result, the proposed methods can achieve better direction-finding performance in the condition of limited snapshots with lower computational cost. The corresponding Cramer-Rao bound (CRB) is deduced and the signal-to-noise ratio (SNR) gain obtained by dimension reduction processing is discussed. Simulation results also show the superiority of the proposed method over the existing methods.
Heel Height as an Etiology of Hallux Abductus Valgus Development: An electromagnetic Static and Dynamic First Metatarsophalangeal Joint Study
Authors: Sánchez-Gómez Rubén , Becerro de Bengoa-Vallejo Ricardo , Losa-Iglesias Marta Elena , Calvo-Lobo César , Romero-Morales Carlos , Martínez-Jiménez Eva María , Palomo-López Patricia , López-López Daniel
Published: March 2019

Background: Hallux abductus valgus (HAV) is a forefoot condition produced by extrinsic and intrinsic factors. Shoes with a high heel height and a typical narrow tip toe box can induce deviations in both the proximal phalanx of the hallux (PPH) and the first metatarsal (IMTT) bones. Nevertheless, the isolated role of heel height remains unclear in the development of HAV pathology. Objectives: The goal was to determine if the heel height increase of shoes without a narrow box toe could augment the PPH and IMTT deviation in frontal, sagittal, and transverse planes toward the first metatarsophalangeal joint (MPJ) and the first metatarsocuneiform joint (MCJ), respectively, during static and dynamic conditions in relation to precursor movements of HAV. Methods: Women with an average age of 25.10 ± 4.67 years were recruited in this cross-sectional study to assess the three planes of motion of PPH and IMTT while wearing high heels with heights at 3, 6, 9 cm and unshod conditions via sandals. The measurements used an electromagnetic goniometer device with sensors placed on medial aspects of the PPH and IMTT bones under static and dynamic conditions. Results: Wearing shoes with a 6 cm heel in dynamic condition may increase the PPH valgus and abduction deviation from 3.15 ± 0.10° to 3.46 ± 0.05° (p < 0.05) and from 1.35 ± 0.28° to 1.69 ± 0.30° (p < 0.001), respectively. In addition, a PPH abduction increase from 1.01 ± 0.36° to 1.31 ± 0.46° (p < 0.05) after wearing shoes with a 6 cm heel height was observed under static conditions. Conclusions: Wearing shoes with a heel height of 6 cm without a narrow box toe interference may produce PPH abduction and valgus deviations related to HAV formation.