bluuebunny/crossref_metadata_2025 huggingface dataset:
Fingolimod Inhibits Exopolysaccharide Production and Regulates Relevant Genes to Eliminate the Biofilm of K. pneumoniae
Authors: Geng Xiang , Yang Ya-Jun , Li Zhun , Ge Wen-Bo , Xu Xiao , Liu Xi-Wang , Li Jian-Yong
Published: January 2024

Klebsiella pneumoniae (K. pneumoniae) exhibits the ability to form biofilms as a means of adapting to its adverse surroundings. K. pneumoniae in this biofilm state demonstrates remarkable resistance, evades immune system attacks, and poses challenges for complete eradication, thereby complicating clinical anti-infection efforts. Moreover, the precise mechanisms governing biofilm formation and disruption remain elusive. Recent studies have discovered that fingolimod (FLD) exhibits biofilm properties against Gram-positive bacteria. Therefore, the antibiofilm properties of FLD were evaluated against multidrug-resistant (MDR) K. pneumoniae in this study. The antibiofilm activity of FLD against K. pneumoniae was assessed utilizing the Alamar Blue assay along with confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), and crystal violet (CV) staining. The results showed that FLD effectively reduced biofilm formation, exopolysaccharide (EPS), motility, and bacterial abundance within K. pneumoniae biofilms without impeding its growth and metabolic activity. Furthermore, the inhibitory impact of FLD on the production of autoinducer-2 (AI-2) signaling molecules was identified, thereby demonstrating its notable anti-quorum sensing (QS) properties. The results of qRT-PCR analysis demonstrated that FLD significantly decreased the expression of genes associated with the efflux pump gene (AcrB, kexD, ketM, kdeA, and kpnE), outer membrane (OM) porin proteins (OmpK35, OmpK36), the quorum-sensing (QS) system (luxS), lipopolysaccharide (LPS) production (wzm), and EPS production (pgaA). Simultaneously, FLD exhibited evident antibacterial synergism, leading to an increased survival rate of G. mellonella infected with MDR K. pneumoniae. These findings suggested that FLD has substantial antibiofilm properties and synergistic antibacterial potential for colistin in treating K. pneumoniae infections.
Unique Interactions of the Small Translocases of the Mitochondrial Inner Membrane (Tims) in Trypanosoma brucei
Authors: Quiñones Linda S. , Gonzalez Fidel Soto , Darden Chauncey , Khan Muhammad , Tripathi Anuj , Smith Joseph T. , Davis Jamaine , Misra Smita , Chaudhuri Minu
Published: January 2024

The infectious agent for African trypanosomiasis, Trypanosoma brucei, possesses a unique and essential translocase of the mitochondrial inner membrane, known as the TbTIM17 complex. TbTim17 associates with six small TbTims (TbTim9, TbTim10, TbTim11, TbTim12, TbTim13, and TbTim8/13). However, the interaction patterns of these smaller TbTims with each other and TbTim17 are not clear. Through yeast two-hybrid (Y2H) and co-immunoprecipitation analyses, we demonstrate that all six small TbTims interact with each other. Stronger interactions were found among TbTim8/13, TbTim9, and TbTim10. However, TbTim10 shows weaker associations with TbTim13, which has a stronger connection with TbTim17. Each of the small TbTims also interacts strongly with the C-terminal region of TbTim17. RNAi studies indicated that among all small TbTims, TbTim13 is most crucial for maintaining the steady-state levels of the TbTIM17 complex. Further analysis of the small TbTim complexes by size exclusion chromatography revealed that each small TbTim, except for TbTim13, is present in ~70 kDa complexes, possibly existing in heterohexameric forms. In contrast, TbTim13 is primarily present in the larger complex (>800 kDa) and co-fractionates with TbTim17. Altogether, our results demonstrate that, relative to other eukaryotes, the architecture and function of the small TbTim complexes are specific to T. brucei.
Canonical and Non-Canonical Localization of Tight Junction Proteins during Early Murine Cranial Development
Authors: Mak Shermin , Hammes Annette
Published: January 2024

This study investigates the intricate composition and spatial distribution of tight junction complex proteins during early mouse neurulation. The analyses focused on the cranial neural tube, which gives rise to all head structures. Neurulation brings about significant changes in the neuronal and non-neuronal ectoderm at a cellular and tissue level. During this process, precise coordination of both epithelial integrity and epithelial dynamics is essential for accurate tissue morphogenesis. Tight junctions are pivotal for epithelial integrity, yet their complex composition in this context remains poorly understood. Our examination of various tight junction proteins in the forebrain region of mouse embryos revealed distinct patterns in the neuronal and non-neuronal ectoderm, as well as mesoderm-derived mesenchymal cells. While claudin-4 exhibited exclusive expression in the non-neuronal ectoderm, we demonstrated a neuronal ectoderm specific localization for claudin-12 in the developing cranial neural tube. Claudin-5 was uniquely present in mesenchymal cells. Regarding the subcellular localization, canonical tight junction localization in the apical junctions was predominant for most tight junction complex proteins. ZO-1 (zona occludens protein-1), claudin-1, claudin-4, claudin-12, and occludin were detected at the apical junction. However, claudin-1 and occludin also appeared in basolateral domains. Intriguingly, claudin-3 displayed a non-canonical localization, overlapping with a nuclear lamina marker. These findings highlight the diverse tissue and subcellular distribution of tight junction proteins and emphasize the need for their precise regulation during the dynamic processes of forebrain development. The study can thereby contribute to a better understanding of the role of tight junction complex proteins in forebrain development.
Phototoxic Reactions Inducted by Hydrochlorothiazide and Furosemide in Normal Skin Cells—In Vitro Studies on Melanocytes and Fibroblasts
Authors: Karkoszka Marta , Rok Jakub , Rzepka Zuzanna , Banach Klaudia , Kowalska Justyna , Wrześniok Dorota
Published: January 2024

Hypertension is known to be a multifactorial disease associated with abnormalities in neuroendocrine, metabolic, and hemodynamic systems. Poorly controlled hypertension causes more than one in eight premature deaths worldwide. Hydrochlorothiazide (HCT) and furosemide (FUR), being first-line drugs in the treatment of hypertension, are among others the most frequently prescribed drugs in the world. Currently, many pharmacoepidemiological data associate the use of these diuretics with an increased risk of adverse phototoxic reactions that may induce the development of melanoma and non-melanoma skin cancers. In this study, the cytotoxic and phototoxic potential of HCT and FUR against skin cells varied by melanin pigment content was assessed for the first time. The results showed that both drugs reduced the number of metabolically active normal skin cells in a dose-dependent manner. UVA irradiation significantly increased the cytotoxicity of HCT towards fibroblasts by approximately 40% and melanocytes by almost 20% compared to unirradiated cells. In the case of skin cells exposed to FUR and UVA radiation, an increase in cytotoxicity by approximately 30% for fibroblasts and 10% for melanocytes was observed. Simultaneous exposure of melanocytes and fibroblasts to HCT or FUR and UVAR caused a decrease in cell viability, and number, which was confirmed by microscopic assessment of morphology. The phototoxic effect of HCT and FUR was associated with the disturbance of redox homeostasis confirming the oxidative stress as a mechanism of phototoxic reaction. UVA-irradiated drugs increased the generation of ROS by 10–150%, and oxidized intracellular thiols. A reduction in mitochondrial potential of almost 80% in melanocytes exposed to HCT and UVAR and 60% in fibroblasts was found due to oxidative stress occurrence. In addition, HCT and FUR have been shown to disrupt the cell cycle of normal skin cells. Finally, it can be concluded that HCT is the drug with a stronger phototoxic effect, and fibroblasts turn out to be more sensitive cells to the phototoxic effect of tested drugs.
The Analysis of Embryoid Body Formation and Its Role in Retinal Organoid Development
Authors: Heredero Berzal Andrea , Wagstaff Ellie L. , ten Asbroek Anneloor L. M. A. , ten Brink Jacoline B. , Bergen Arthur A. , Boon Camiel J. F.
Published: January 2024

Within the last decade, a wide variety of protocols have emerged for the generation of retinal organoids. A subset of studies have compared protocols based on stem cell source, the physical features of the microenvironment, and both internal and external signals, all features that influence embryoid body and retinal organoid formation. Most of these comparisons have focused on the effect of signaling pathways on retinal organoid development. In this study, our aim is to understand whether starting cell conditions, specifically those involved in embryoid body formation, affect the development of retinal organoids in terms of differentiation capacity and reproducibility. To investigate this, we used the popular 3D floating culture method to generate retinal organoids from stem cells. This method starts with either small clumps of stem cells generated from larger clones (clumps protocol, CP) or with an aggregation of single cells (single cells protocol, SCP). Using histological analysis and gene-expression comparison, we found a retention of the pluripotency capacity on embryoid bodies generated through the SCP compared to the CP. Nonetheless, these early developmental differences seem not to impact the final retinal organoid formation, suggesting a potential compensatory mechanism during the neurosphere stage. This study not only facilitates an in-depth exploration of embryoid body development but also provides valuable insights for the selection of the most suitable protocol in order to study retinal development and to model inherited retinal disorders in vitro.
How to Use the Cuprizone Model to Study De- and Remyelination
Authors: Kipp Markus
Published: January 2024

Multiple sclerosis (MS) is an autoimmune and inflammatory disorder affecting the central nervous system whose cause is still largely unknown. Oligodendrocyte degeneration results in demyelination of axons, which can eventually be repaired by a mechanism called remyelination. Prevention of demyelination and the pharmacological support of remyelination are two promising strategies to ameliorate disease progression in MS patients. The cuprizone model is commonly employed to investigate oligodendrocyte degeneration mechanisms or to explore remyelination pathways. During the last decades, several different protocols have been applied, and all have their pros and cons. This article intends to offer guidance for conducting pre-clinical trials using the cuprizone model in mice, focusing on discovering new treatment approaches to prevent oligodendrocyte degeneration or enhance remyelination.
Initial Oxidation Behavior of AlCoCrFeNi High-Entropy Coating Produced by Atmospheric Plasma Spraying in the Range of 650 °C to 1000 °C
Authors: Chen Rong , You Xin , Ren Ke , Liang Yuwei , Huang Taihong , Zheng Biju , Song Peng
Published: January 2024

As protective coatings for the thermal parts of aero-engines, AlCoCrFeNi coatings have good application prospects. In this study, atmospheric plasma spraying (APS) was used to prepare AlCoCrFeNi high-entropy coatings (HECs), which were oxidized from 650 °C to 1000 °C. The mechanism of the oxide layer formation and the internal phase transition were systematically investigated. The results show that a mixed oxide scale with a laminated structure was formed at the initial stage of oxidation. The redistribution of elements and phase transition occurred in the HECs’ matrix; the BCC/B2 structure transformed to Al-Ni ordered B2 phase and Fe-Cr disordered A2 phase.
In-Plane Anisotropy of Electrical Transport in Y0.85Tb0.15Ba2Cu3O7−x Films
Authors: Lyatti Matvey , Kraiem Ines , Röper Torsten , Gundareva Irina , Mussler Gregor , Jalil Abdur Rehman , Grützmacher Detlev , Schäpers Thomas
Published: January 2024

We fabricated high-quality c-axis-oriented epitaxial YBa2Cu3O7−x films with 15% of the yttrium atoms replaced by terbium (YTBCO) and studied their electrical properties. The Tb substitution reduced the charge carrier density, resulting in increased resistivity and decreased critical current density compared to pure YBa2Cu3O7−x films. The electrical properties of the YTBCO films showed an in-plane anisotropy in both the superconducting and normal states that, together with the XRD data, provided evidence for, at least, a partially twin-free film. Unexpectedly, the resistive transition of the bridges also demonstrated the in-plane anisotropy that could be explained within the framework of Tinkham’s model of resistive transition and the Berezinskii–Kosterlitz–Thouless (BKT) model, depending on the sample parameters. Measurements of the differential resistance in the temperature range of the resistive transition confirmed the occurrence of the BKT transition in the YTBCO bridges. Therefore, we consider the YTBCO films to be a promising platform for both the fabrication of devices with high kinetic inductance and fundamental research on the BKT transition in cuprate superconductors.
Comparative Evaluation of the Antibacterial and Antitumor Activities of 9-Phenylfascaplysin and Its Analogs
Authors: Zhidkov Maxim E. , Sidorova Maria A. , Smirnova Polina A. , Tryapkin Oleg A. , Kachanov Andrey V. , Kantemirov Alexey V. , Dezhenkova Lyubov G. , Grammatikova Natalia E. , Isakova Elena B. , Shchekotikhin Andrey E. , Pak Marina A. , Styshova Olga N. , Klimovich Anna A. , Popov Aleksandr M.
Published: January 2024

Based on the results of our own preliminary studies, the derivative of the marine alkaloid fascaplysin containing a phenyl substituent at C-9 was selected to evaluate the therapeutic potential in vivo and in vitro. It was shown that this compound has outstandingly high antimicrobial activity against Gram-positive bacteria, including antibiotic-resistant strains in vitro. The presence of a substituent at C-9 of the framework is of fundamental importance, since its replacement to neighboring positions leads to a sharp decrease in the selectivity of the antibacterial action, which indicates the presence of a specific therapeutic target in bacterial cells. On a model of the acute bacterial sepsis in mice, it was shown that the lead compound was more effective than the reference antibiotic vancomycin seven out of nine times. However, ED50 value for 9-phenylfascaplysin (7) was similar for the unsubstituted fascaplysin (1) in vivo, despite the former being significantly more active than the latter in vitro. Similarly, assessments of the anticancer activity of compound 7 against various variants of Ehrlich carcinoma in mice demonstrated its substantial efficacy. To conduct a structure–activity relationship (SAR) analysis and searches of new candidate compounds, we synthesized a series of analogs of 9-phenylfascaplysin with varying aryl substituents. However, these modifications led to the reduced aqueous solubility of fascaplysin derivatives or caused a loss of their antibacterial activity. As a result, further research is required to explore new avenues for enhancing its pharmacokinetic characteristics, the modification of the heterocyclic framework, and optimizing of treatment regimens to harness the remarkable antimicrobial potential of fascaplysin for practical usage.
Dual Field-of-View Off-Axis Spatially Multiplexed Digital Holography Using Fresnel’s Bi-Mirror
Authors: Pensia Lavlesh , Kumar Manoj , Kumar Raj
Published: January 2024

Digital holography (DH) is an important method for three-dimensional (3D) imaging since it allows for the recording and reconstruction of an object’s amplitude and phase information. However, the field of view (FOV) of a DH system is typically restricted by the finite size of the pixel pitch of the digital image sensor. We proposed a new configuration of the DH system based on Fresnel’s bi-mirror to achieve doubling the camera FOV of the existing off-axis DH system which leveraged single-shot acquisition and a common-path optical framework. The dual FOV was obtained by spatial frequency multiplexing corresponding to two different information-carrying beams from an object. Experimental evidence of the proposed dual FOV-DH system’s viability was provided by imaging two different areas of the test object and an application to surface profilometry by measuring the step height of the resolution chart which showed excellent agreement with an optical profiler. Due to the simple configuration, the proposed system could find a wide range of applications, including in microscopy and optical metrology.
Wide-Angle Beam Steering Closed-Form Pillbox Antenna Fed by Substrate-Integrated Waveguide Horn for On-the-Move Satellite Communications
Authors: Ikram Muhammad , Sultan Kamel , Mobashsher Ahmed Toaha , Moosazadeh Mahdi , Abbosh Amin
Published: January 2024

Wide-angle mechanical beam steering for on-the-move satellite communications is presented in this paper based on a closed-form pillbox antenna system. It includes three main parts: a fixed-feed part, which is a substrate-integrated waveguide (SIW) horn with an extended aperture attached to a parabolic reflector; a novel quasi-optical system, which is a single coupling slot alongside and without spacing from the parabolic reflector; and a radiating disc, which is a leaky-wave metallic pattern. To make the antenna compact, pillbox-based feeding is implemented underneath the metallic patterns. The antenna is designed based on a substrate-guided grounded concept using leaky-wave metallic patterns operating at 20 GHz. Beam scanning is achieved using mechanical rotation of the leaky-wave metallic patterns. The proposed antenna has an overall size of 340 × 335 × 2 mm3, a gain of 23.2 dBi, wide beam scanning range of 120°, from −60° to +60° in the azimuthal plane, and a low side lobe level of −17.8 dB at a maximum scan angle of 60°. The proposed antenna terminal is suitable for next-generation ubiquitous connectivity for households and small businesses in remote areas, ships, unmanned aerial vehicles, and disaster management.
A Wearable Inertial Sensor Approach for Locomotion and Localization Recognition on Physical Activity
Authors: Khan Danyal , Al Mudawi Naif , Abdelhaq Maha , Alazeb Abdulwahab , Alotaibi Saud S. , Algarni Asaad , Jalal Ahmad
Published: January 2024

Advancements in sensing technology have expanded the capabilities of both wearable devices and smartphones, which are now commonly equipped with inertial sensors such as accelerometers and gyroscopes. Initially, these sensors were used for device feature advancement, but now, they can be used for a variety of applications. Human activity recognition (HAR) is an interesting research area that can be used for many applications like health monitoring, sports, fitness, medical purposes, etc. In this research, we designed an advanced system that recognizes different human locomotion and localization activities. The data were collected from raw sensors that contain noise. In the first step, we detail our noise removal process, which employs a Chebyshev type 1 filter to clean the raw sensor data, and then the signal is segmented by utilizing Hamming windows. After that, features were extracted for different sensors. To select the best feature for the system, the recursive feature elimination method was used. We then used SMOTE data augmentation techniques to solve the imbalanced nature of the Extrasensory dataset. Finally, the augmented and balanced data were sent to a long short-term memory (LSTM) deep learning classifier for classification. The datasets used in this research were Real-World Har, Real-Life Har, and Extrasensory. The presented system achieved 89% for Real-Life Har, 85% for Real-World Har, and 95% for the Extrasensory dataset. The proposed system outperforms the available state-of-the-art methods.
Compact and Fully Integrated LED Quantum Sensor Based on NV Centers in Diamond
Authors: Pogorzelski Jens , Horsthemke Ludwig , Homrighausen Jonas , Stiegekötter Dennis , Gregor Markus , Glösekötter Peter
Published: January 2024

Quantum magnetometry based on optically detected magnetic resonance (ODMR) of nitrogen vacancy centers in diamond nano or microcrystals is a promising technology for sensitive, integrated magnetic-field sensors. Currently, this technology is still cost-intensive and mainly found in research. Here we propose one of the smallest fully integrated quantum sensors to date based on nitrogen vacancy (NV) centers in diamond microcrystals. It is an extremely cost-effective device that integrates a pump light source, photodiode, microwave antenna, filtering and fluorescence detection. Thus, the sensor offers an all-electric interface without the need to adjust or connect optical components. A sensitivity of 28.32nT/Hz and a theoretical shot noise limited sensitivity of 2.87 nT/Hz is reached. Since only generally available parts were used, the sensor can be easily produced in a small series. The form factor of (6.9 × 3.9 × 15.9) mm3 combined with the integration level is the smallest fully integrated NV-based sensor proposed so far. With a power consumption of around 0.1W, this sensor becomes interesting for a wide range of stationary and handheld systems. This development paves the way for the wide usage of quantum magnetometers in non-laboratory environments and technical applications.
Electrochemical Sensors Based on Transition Metal Materials for Phenolic Compound Detection
Authors: Amorim Isilda , Bento Fátima
Published: January 2024

Electrochemical sensors have been recognized as crucial tools for monitoring comprehensive chemical information, especially in the detection of a significant class of molecules known as phenolic compounds. These compounds can be present in water as hazardous analytes and trace contaminants, as well as in living organisms where they regulate their metabolism. The sensitive detection of phenolic compounds requires highly efficient and cost-effective electrocatalysts to enable the development of high-performance sensors. Therefore, this review focuses on the development of advanced materials with excellent catalytic activity as alternative electrocatalysts to conventional ones, with a specific emphasis on transition metal-based electrocatalysts for the detection of phenolic compounds. This research is particularly relevant in diverse sectors such as water quality, food safety, and healthcare.
Torsobarography: Intra-Observer Reliability Study of a Novel Posture Analysis Based on Pressure Distribution
Authors: Stecher Nico , Heinke Andreas , Żurawski Arkadiusz Łukasz , Harder Maximilian Robert , Schumann Paula , Jochim Thurid , Malberg Hagen
Published: January 2024

Postural deformities often manifest themselves in a sagittal imbalance and an asymmetric morphology of the torso. As a novel topographic method, torsobarography assesses the morphology of the back by analysing pressure distribution along the torso in a lying position. At torsobarography’s core is a capacitive pressure sensor array. To evaluate its feasibility as a diagnostic tool, the reproducibility of the system and extracted anatomical associated parameters were evaluated on 40 subjects. Landmarks and reference distances were identified within the pressure images. The examined parameters describe the shape of the spine, various structures of the trunk symmetry, such as the scapulae, and the pelvic posture. The results showed that the localisation of the different structures performs with a good (ICC > 0.75) to excellent (ICC > 0.90) reliability. In particular, parameters for approximating the sagittal spine shape were reliably reproduced (ICC > 0.83). Lower reliability was observed for asymmetry parameters, which can be related to the low variability within the subject group. Nonetheless, the reliability levels of selected parameters are comparable to commercial systems. This study demonstrates the substantial potential of torsobarography at its current stage for reliable posture analysis and may pave the way as an early detection system for postural deformities.
Effect of Zoapatle (Montanoa tomentosa) on Inflammatory Markers in a Murine Model of Ventricular Hypertrophy
Authors: López-Luna Carlos Enrique , Vargas-De-León Cruz , Gutiérrez-Rojas Rocio Alejandra , Aguayo-Cerón Karla Aidee , Calzada-Mendoza Claudia Camelia , Huang Fengyang , Romero-Nava Rodrigo , Ocharan-Hernandez Maria Esther
Published: January 2024

Zoapatle, a native plant utilized for centuries in traditional Mexican medicine, is abundantly found in Mesoamerica and northern South America. Pleiotropic effects of this genus have been recognized, primarily inducing alterations in smooth muscle contractility in animal models. The aim of this study was to evaluate the effect of Zoapatle on the hypertrophy index and the gene expression of TNF-α, IL-1β, NF-κB, STAT5, and the PRLR in the brain, left ventricle, and renal cortex of rats with isoproterenol-induced cardiac hypertrophy. Three groups were studied, the control group (n = 4), hypertrophy group (n = 4) and hypertrophy group treated with Zoapatle (n = 4). A ventricular hypertrophy model was developed with 150 mg/kg/day of isoproterenol intraperitoneally administered over two days with a 24 h interval between applications. Zoapatle was administered for 28 consecutive days (25 mg/kg). Gene expression was determined with RT-qPCR. Subsequently, a principal component analysis (PCA) was performed using the RNA expression variables. A notably reduced left ventricle mass index was observed in the Zoapatle group. Additionally, Zoapatle administration in cardiac hypertrophy demonstrated a significant decrease in the gene expression of TNF-α, IL-1B, STAT 5, and the PRLR. TNF-α and the transcription factor STAT5 exhibited a similar trend in both the left ventricle and renal cortex, suggesting a correlation with the inflammatory state in these tissues due to ventricular hypertrophy. The findings suggest that Zoapatle reverses the hypertrophy index in a hypertrophy model, concurrently reducing several proinflammatory mediators associated with the hypertrophy index.
Makna Panggilan Surgawi Menurut Filipi 3:10-14 dan Aplikasinya bagi Mahasiswa Teologi
Authors: Simanjuntak Elisa Septian Suandana , Sumiwi Asih Rachmani Endang , Prabowo Yusak Sigit
Published: January 2024

In responding to heavenly calling, a person must realize that God created him as a unique person with different abilities and skills. God also calls each person to do His will uniquely and specially. Theology students face challenges that are not easy. However, they must understand and understand the heavenly calling to overcome the challenge. This research uses qualitative methods with a hermeneutic approach. This approach is done to find the meaning of heavenly vocation according to Philippians 3:10-14 and how it applies to students of theology. Theology students are people who have answered God's call to learn about God. They come from a variety of economic, cultural, and tribal backgrounds. The meaning of heavenly calling according to Philippians 3:10-14 is to forget the past, turn to the heavenly call, and run to the destination for the reward. The application of the meaning of the heavenly vocation for theological students is as follows: Theology students do not live in the past but focus on heavenly vocations; Theology students have purposeful, goal-focused lives; Theology students are persistent in discovering heavenly callings. Dalam menanggapi panggilan surgawi, seseorang harus menyadari bahwa Allah menciptakannya sebagai pribadi yang unik dengan kemampuan dan keahlian yang berbeda-beda. Allah juga memanggil setiap orang untuk menjalankan kehendak-Nya secara unik dan spesial. Mahasiswa teologi menghadapi tantangan yang tidak mudah. Namun, mereka harus mengerti dan memahami panggilan surgawi agar dapat mengatasi tantangan tersebut. Penelitian ini menggunakan metode kualitatif dengan pendekatan hermeneutik. Pendekatan ini dilakukan untuk mencari makna panggilan surgawi menurut Filipi 3:10-14 dan bagaimana aplikasinya bagi mahasiswa teologi. Mahasiswa teologi adalah orang-orang yang telah menjawab panggilan Tuhan untuk mempelajari tentang Allah. Mereka berasal dari berbagai latar belakang ekonomi, budaya, dan suku. Makna panggilan surgawi menurut Filipi 3:10-14 adalah untuk melupakan masa lalu, mengarahkan diri pada panggilan surgawi, dan berlari-lari kepada tujuan untuk memperoleh hadiah. Aplikasi makna panggilan surgawi bagi mahasiswa teologi adalah sebagai berikut: Mahasiswa teologi tidak hidup di masa lalu, tetapi fokus pada panggilan surgawi; mahasiswa teologi memiliki hidup yang terarah dan berfokus pada tujuan; mahasiswa teologi gigih dalam menemukan panggilan surgawi.
Developing Kruger Shalati: The Train on the Bridge
Authors: Noordzy Gert , Whitfield Richard , Lin Li-Chun , Makhudu Tshepo
Published: January 2024

This is a case study on the development of the Kruger Shalati: The Train on the Bridge, a themed, luxury boutique hotel in Kruger National Park (KNP), South Africa. Two key drivers of this project were authenticity and a strong ethos for Environmental, Social and Governance (ESG). Considering the target market the hotel serves and the local situation, this case study explains why modular construction was better to build parts of the property, instead of using traditional in situ construction methods. It further explains how the hotel was built, and the challenges that had to be addressed to complete the project. Finally, it examines how the use of modular construction methods and the uniqueness of the project impacted the normal hotel pre-opening activities and ongoing hotel operations. This case study has been prepared after reviewing published materials about the project and extensively interviewing five senior members of the project team.
Social and demographic factors in shaping the perception of work as a source of health and safety risks: a study on adult Europeans
Authors: Roszko-Wójtowicz Elżbieta , Boczkowska Katarzyna , Niziołek Konrad
Published: January 2024

The aim of this article is to assess the impact of selected social and demographic factors on the perception of European adults regarding their workplace as a health and safety risk. This aligns with the sustainable development concept, which emphasizes labor rights protection and a safe working environment. Sustainable work is defined as work that doesn't compromise employees' physical or mental health over time. Utilizing data from the 2021 European Working Conditions Survey, which covered over 70,000 individuals across 36 countries and was conducted via CATI due to the pandemic, the study employs logistic regression. It analyzes three models: one encompassing all European countries, and two focusing on Eastern and Central European countries. The findings demonstrate that factors such as company size, age, occupational group, sector, employment nature, gender, service length, and education significantly influence workplace risk perception. International comparisons highlight differences in these factors across country groups, contributing to the scientific discussion in social sciences.
PENGARUH PEMBERIAN JAHE HANGAT TERHADAP FREKUENSI MUAL MUNTAH PADA IBU HAMIL TRIMESTER I DI DESA TAMBUSAI BATANG DUI KECAMATAN BATHIN SOLAPAN.
Authors: Agustina Santi , Diana Nana , Oktora Sri Rahayu Ary
Published: January 2024

Mual muntah merupakan keluhan normal yang sering dialami ibu hamil trimester pertama, dan dapat menjadi hiperemesis gravidarum sehingga meningkatkan resiko terjadinya gangguan kehamilan. Aromaterapi merupakan terapi modalitas atau pengobatan alternative dengan sari tumbuhan. Sedangkan jahe merupakan tanaman herbal yang sudah sejak lama dikenal untuk gangguan kehamilan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian jahe hangat terhadap frekuensi mual muntah. Desain penelitian ini adalah quasi experiment dengan rancangan pre test dan post test. Hasil penelitian Rata-rata frekuensi mual muntah sebelum diberikan jahe hangat rata-rata frekuensi mual muntah sebelum diberikan adalah 6,47 dengan p-value 0,271(>0,05) dan sesudah diberikan jahe hangat 3,67 dengan p-value 0,230 (>0,05) diasumsikan frekuensi mual muntah pada ibu hamil terdistribusi normal. Penelitian ini dilakukan diwilayah Desa Tambusai Batang Dui Kecamatan Bathin Solapan Kabupeten Bengkalis. Untuk itu penting kiranya tenaga kesehatan memperkenalkan terapi non-farmokologis seperti jahe hangat sebagai antimetik yang paling aman menangani mual muntah
MOBILE TECHNOLOGY ADOPTION AND ITS DIRECTION TOWARD A FUTURE CAREER IN BROADCASTING AND JOURNALISM AS A SOLO BROADCAST JOURNALIST
Authors: Abu Talib Zuraidah , Saberi Syamimi
Published: January 2024

This paper aims to delve into the adoption of mobile technology among the university's young generation majoring in broadcasting and journalism. Further discussion will translate the mobile adoption and its direction toward the future of solo broadcasting journalism in Malaysia, respectively among them. During the pandemic covid 19, people have adapted to tremendous changes in their everyday lives. Since then, the Internet of Things and digitalisation have become new norms since they must access everything from home. In other words, technology has become essential to everyone despite their age, gender, or location. Almost everyone can broadcast and become a journalist, as long as they know and have skills in using various technologies ranging from email to web blogs to digital videos to mobile technologies to social media. In line with this modernisation, the solo broadcast journalist has also been identified as an active mobile device user to fulfil their job preference. Solo broadcasting journalism has become essential in the world of broadcast and journalism. A person who wants to work in this convergence field must have the ability and knowledge to handle all mobile technology devices alone when they have to do fieldwork. However, ability and knowledge are not the main points; other factors can motivate a person to learn and become a solo journalist. Therefore, this research explores the factors influencing the desire to use mobile devices among 200 broadcasting and journalism students at Universiti Utara Malaysia, Sintok Kedah. The present study indicates that perceived ease of use, perceived usefulness, and experience positively correlated with using mobile technologies. The study mainly contributes to mobile technologies and social media adoption among the university's young generation majoring in broadcasting and journalism to prepare for their future broadcasting and journalism careers.
COMMUNICATION STRATEGIES, COMMUNICATION BARRIERS, ORGANIZATIONAL COMMITMENT AND CUSTOMER SATISFACTION AMONG THE INTERNAL CUSTOMER
Authors: Ramli Romlah , Mehat Nor Aziah
Published: January 2024

This paper analyzes the effect between communication strategy, communication barriers, organizational commitment, and customer satisfaction in service provision. Data collection was conducted to 308 internal clients at a local University. The data of this study was analyzed using descriptive statistics, correlation analysis, and regression analysis through the Statistical Package for the Social Sciences. The results of the analysis show that communication strategies and organizational commitment have a significant effect on the level of customer satisfaction, while communication barriers do not contribute to customer satisfaction. In conclusion, the organization needs to improve its strategy and organizational commitment that is more focused in increasing customer satisfaction.
A SURVEY OF BLUEBLUE INFORMATION DESIGN'S PROJECT EXAMPLES, DESIGN METHODOLOGIES, AND TOOLS IN THE FIELD OF INFORMATION VISUALIZATION
Authors: Zhang Huajin , Mohammed Ali Noor Azly , Che Cob Saiful Akram , Chu Fan , Li Yifei
Published: January 2024

The aim of this thesis is to provide an in-depth study of case studies of Blue Blue Information Design's projects in the field of data visualisation, as well as the infographics design methodologies and tools employed when dealing with large amounts of data. By analysing actual projects, the researcher explores how information design firms can improve data communication through innovative data visualisation tools and the best practices adopted when dealing with large-scale data.Using case studies, non-participant observation, and interviews, this study focuses on the experiences and innovative approaches of commercial design firms.BlueBlue Design demonstrates excellence in information design through a systematic and structured design process, user-centred approach, innovative visual solutions, data-driven decision-making, and multimedia integration. Their emphasis on collaboration and communication with clients and teams ensures that design solutions meet expectations during the course of a project, reflecting efficient and close internal and external collaboration.
MYUMRAH: A DIGITAL UMRAH MOBILE GUIDE APPLICATION
Authors: Saidin Aeni Zuhana , Mazlan Nur Dania
Published: January 2024

Umrah, a sacred ritual in Islam, holds significant religious importance as believers seek to fulfill their faith. This article presents the development of the MyUmrah application, designed to assist pilgrims in their Umrah journey both before and during the performance of the ritual. This application encompasses various functions tailored to meet the needs of Umrah pilgrims. The development of MyUmrah follows an iterative application development process, beginning with the collection of data to establish requirements followed by design and development of the prototype that aligns with the identified requirements. To gain insights into the newly developed app, a prototype evaluation is conducted using dichotomous techniques, providing a comprehensive understanding of its functionality and effectiveness. The feedback received during the evaluation phase have been overwhelmingly positive, spanning various aspects of usefulness, usage, and design. This indicates a high level of acceptance and satisfaction among Umrah pilgrims. Users have reported positive experiences, affirming the application's effectiveness in facilitating a smooth Umrah experience. MyUmrah application proves to be beneficial, offering valuable assistance to pilgrims throughout their sacred journey.
MEWUJUDKAN KOTA LAYAK ANAK BERBASIS KEBUTUHAN DI KOTA KENDARI
Authors: Dewi Anggraini  , La Ode Muhammad Elwan  , M. Najib Husain
Published: January 2024

This research aims to evaluate the city components that support Kendari City's condition as a Child-Friendly City and analyze the factors that hinder and support this status. A qualitative descriptive method with an ethnographic study approach was used to identify city components such as the residential environment, community environment, school environment, play areas, transportation and health services that support the condition of Kendari City as a Child-Friendly City. The research results show that there are several obstacle factors such as infrastructure problems that affect children's access to education and health services. This evaluation provides a holistic picture of Kendari City's efforts to create a conducive environment for children's development and offers a perspective regarding the challenges that need to be overcome to achieve optimal Child-Friendly City status.
A Review Article of the Pragmatics Competence in EFL Learning Context
Authors: Amaliah Amaliah
Published: January 2024

Cross-cultural communication requires pragmatics. Pragmatics is "the ability to use language appropriately in social settings." Pragmatic competence in another language involves understanding and carrying out communicative activities or speaking and using the language effectively in varied situations. Several relevant literature and library sources were used for this research. According to Kasper et al. "Library research, like its twin, the academic experiment, involves a kind of organized investigation with prescribed instruments, rules, and tactics." Three core library research systems are examined: checking reference sources and databases, finding and utilizing information sources, and assessing information sources according to research objectives. This article analyzes data on pragmatic competence in students of Indonesian and other foreign languages. The author used a public digital search engine to examine scientific literature on pragmatic competency studies in Indonesia to correlate disparate data and find appropriate results. Qualitative research may be more comprehensive. This study found that most studies used a descriptive qualitative approach. The exam is detailed. Most studies used a qualitative approach to characterize L2 practical skills by level and test, while one study assessed learners' cross-cultural awareness during encounters. Most studies on educational participation and the impact of cross-cultural awareness on pragmatic knowledge in EFL learning use qualitative methods, although there are three studies that use quantitative methods. Reviews and debates provide suggestions. Recent research on pragmatic competence in EFL learning settings features a descriptive qualitative approach complemented by Creswell's qualitative approach that provides greater detail about the phenomenon, and all three studies use quantitative methodology. Pragmatic competence improves EFL students' social skills. Al Khadhmi found that the pragmatic meaning of situational utterances was more difficult to interpret compared to lexical stimuli. Therefore, it is better to continuously improve certain areas, such as training EFL students' awareness before they understand a foreign language and activating pragmatic competence and pragmatic components in them, as this affects their communication inside and outside the classroom
CA-cecum Presenting as Large Bowel Obstruction with Appendicitis: A Rare Case Report
Authors: Choudhary Vijendra Kumar , Hota Parthasarathi
Published: January 2024

Introduction: Caecal tumors, though rare, pose diagnostic challenges due to their insidious onset and anatomical features. This case study emphasises the importance of a high index of suspicion for early diagnosis and treatment of caecal carcinoma. Case Report: A 63-year-old female presented with symptoms suggestive of large bowel obstruction and acute appendicitis. Imaging revealed a tubular structure in the subcecal location, prompting emergency laparotomy. The patient underwent right hemicolectomy, revealing a 6 cm adenocarcinoma involving the terminal ileum, ileocecal junction, and cecum. Surgical biopsy confirmed moderately differentiated infiltrating adenocarcinoma. Discussion: The discussion delves into the increasing incidence of colon cancer in India, particularly in patients presenting with acute appendicitis. Diagnostic challenges, including interpreting diffusely dilated colon images, are explored. Surgical management is detailed, emphasising the need for right hemicolectomy for caecal lesions and various approaches for left-sided lesions. The multidisciplinary approach to patient care, involving medical and radiation oncologists, is highlighted for complex colorectal diseases. Conclusion: This case underscores the significance of considering caecal carcinoma in patients with intestinal obstruction, leading to appropriate diagnostic measures and timely surgical intervention. The importance of a multidisciplinary approach to patient care is crucial for effective
Effect of Weight Training on Increasing 100 Meter Running Speed in Sprinter Athletes Based on Gender
Authors: Permatasari Dhita , Tomoliyus Tomoliyus , Hariono Awan , Prabowo Trisnar Adi
Published: January 2024

The aim of this study was to investigate the effect of weight training on the performance of 100meter spinner athletes based on gender. This research is one group pretest – posttest. Samples on athletes were carried out voluntarily provided that they were not injured and were not taking part in a competition period. The sample consisted of 10 male sprinters (mean: height 170.2±0.6 cm, weight 64.2±2.7, body mass index (BMI) 21.27, training experience 5.6±7.8 years) and 10 female sprinters (mean: height 168.7±7.2, weight 53.7±0.6, body mass index (BMI) 18.14, training experience 5.2±14 years) with age 17 – 22 years. The test for pretest–posttest is 100 meters run, while determining the load volume uses the 1RM Brzycki formula. This training program is carried out for 6 weeks (3 times a week), training volume 40% - 60% with 3 sets x 8 repetitions, types of weight training are bench press, deadlift, leg press, half squat, double leg bounds. The results of the t test, this research shows that weight training has an effect on increasing the 100 meters sprint of male athletes by 0.000 < 0.05 with an increase in time of 0.33 seconds. Then weight training has an effect on increasing the 100 meters sprint of male athletes by 0.000 < 0.05 with an increase in time of 0.20 seconds. The recommendation of this research is to train at the general preparation stage, because this weight training using 2 supports on the right and left sides of the hands and feet.
Comparative Antimicrobial Activity of Securidaca longipedunculata (Fresen) Extracts on Selected Human Pathogens
Authors: G. Hassan L. , J. Umar K. , J. Yusuf A. , B. Rabah A. , U. Umar A.
Published: January 2024

The escalating trend of microbial resistance has become a pressing global concern, challenging the effectiveness of antimicrobial agents in treating infections. This study was aimed at evaluating the antimicrobial activity of the n-hexane, ethyl acetate, and methanol extracts of Securidaca longipedunculata against selected human pathogens. The antimicrobial screening against bacterial pathogens- (e.g., Methicillin-resistant staphylococcus aureus, Vancomycin-resistant Enterococci, Staphylococcus aureus, Staphylococcus feacalis, Escherichia coli, Salmonella typhimurium, Pseudomans fluorescens and Klebsiella pneumonieae) and fungal pathogens (which includes Candida albicans, Candida krusei, Aspergillus niger, Aspergillus fumigates, and Microsporum canis) was performed using agar well diffusion and broth dilution methods. The results demonstrated significant antimicrobial activity, with higher efficacy against S. aureus. Susceptibility test results showed that the extract inhibited the growth of all the test organisms (bacteria and fungi) with a mean zone of inhibition range of 18-29 mm, with the exception of VRE, S. typhimurium, P. fluorescens, C. krusei and M. canis in all the three extracts, while Hexane extract showed no inhibition against E. coli and A. niger. The standard drugs sparfloxacin and ciprofloxacin (500 µg/cm3) had zone of inhibition ranging from 28 - 40 mm. While the antifungal drugs fluconazole and fulcin exhibited zone of inhibition ranging from 27 mm to 34 mm. The Minimum inhibitory concentration (MIC) for the extracts ranges from 62.5 - 250µg/cm3, while the Minimum Bactericidal/Fungicidal Concentrations (MBC/MFC) range from 250 - 500 µg/cm3, respectively. The ethyl acetate extracts possessed higher antimicrobial activity among the three extracts tested against S. Aureus. The findings suggest that S. longipedunculata leaves hold promise as a natural source of antimicrobial agents, warranting further exploration for potential therapeutic applications.