bluuebunny/crossref_metadata_2025 huggingface dataset:
A Thermic Effect on Degradation Kinetics of Sugar Cane Bagasse Polypropylene Composites
Authors: Motaung Tshwafo E. , Motloung Setumo V. , Koao Lehlohonolo F. , Malevu Thembinkosi D. , Linganiso Ella. C.
Published: April 2022

In this study, thermal degradation mechanisms and the kinetics of PP (Polypropylene) composites containing alkali and saline treated SC (Sugar cane bagasse) have been evaluated using a non-isothermal thermogravimetric analysis under consistent nitrogen atmosphere. The study indicates dynamics of kinetics that need to be considered should the composites be applied in high temperature applications. NaOH treated composites revealed a reduced fiber size compared to the other composites. The presence of SC generally reduced the functional group intensities of FTIR peaks, however some peaks re-emerged after the treatments. The composites indicated higher thermal stability and char content than the pristine polymer. In fact, NaOH treated composite is more thermally stable, while the saline is the least stable of the rest. Well known reliable degradation kinetics methods were employed in order to unpack thermal degradation behavior and possible metaphors. Flynn–Wall–Ozawa (FWO) and Kissinger–Akahira–Sunose (KAS) thermal degradation kinetic models are in agreement that the presence of both SC and those in the PP matrix that have been treated lead to increased activation energy values with the competing reactions in the degradation process. Nonetheless, the linear relation is not absolutely perfect and the competing reactions seem complex at lower temperatures as there are overlying inconsistencies in activation energies. Interestingly, bagasse indicated some effect on the mechanism that included the hindering of free radicals that emanated from the first cleavage of PP.
Microwave Imaging for Early Breast Cancer Detection: Current State, Challenges, and Future Directions
Authors: AlSawaftah Nour , El-Abed Salma , Dhou Salam , Zakaria Amer
Published: April 2022

Breast cancer is the most commonly diagnosed cancer type and is the leading cause of cancer-related death among females worldwide. Breast screening and early detection are currently the most successful approaches for the management and treatment of this disease. Several imaging modalities are currently utilized for detecting breast cancer, of which microwave imaging (MWI) is gaining quite a lot of attention as a promising diagnostic tool for early breast cancer detection. MWI is a noninvasive, relatively inexpensive, fast, convenient, and safe screening tool. The purpose of this paper is to provide an up-to-date survey of the principles, developments, and current research status of MWI for breast cancer detection. This paper is structured into two sections; the first is an overview of current MWI techniques used for detecting breast cancer, followed by an explanation of the working principle behind MWI and its various types, namely, microwave tomography and radar-based imaging. In the second section, a review of the initial experiments along with more recent studies on the use of MWI for breast cancer detection is presented. Furthermore, the paper summarizes the challenges facing MWI as a breast cancer detection tool and provides future research directions. On the whole, MWI has proven its potential as a screening tool for breast cancer detection, both as a standalone or complementary technique. However, there are a few challenges that need to be addressed to unlock the full potential of this imaging modality and translate it to clinical settings.
Characterization of the Interaction of Metalworking Fluids with Grinding Wheels
Authors: Schumski Lukas , Guba Nikolai , Espenhahn Björn , Stöbener Dirk , Fischer Andreas , Meyer Daniel
Published: April 2022

The thermal load that occurs during grinding can be reduced with the aid of an optimized metalworking fluid (MWF) supply. In previous work, mainly the free jet was considered for the determination of the conditions required for an optimized MWF supply. An investigation of the interaction area between the MWF and the grinding wheel has not yet been carried out due to the lack of suitable measurement techniques. In the presented work, both the free jet and the interaction area are analyzed with the aid of new metrological analysis and evaluation methods based on high-speed records (shadowgraphy and shadogram imaging velocimetry) in order to assess the free jet geometry and velocities, as well as the velocity distribution and the MWF amount in the interaction area. Using this approach, the following main results were derived: (1) The free jet velocity remains approximately constant in a defined free jet cross-section even at high distances from the nozzle outlet. (2) The velocity distribution in the interaction area is mainly influenced by the flow rate. (3) A new image parameter (black pixel fraction) was derived for the evaluation of the MWF supply to the contact zone.
Effects of Heat Treatment on Microstructure and Mechanical Properties of AlSi10Mg Fabricated by Selective Laser Melting Process
Authors: Clement Catherine Dolly , Masson Julie , Kabir Abu Syed
Published: April 2022

AlSi10Mg is the most widely additively manufactured and commercialized aluminum alloy and has been used in this study to analyze the effect of heat treatment on its microstructure and mechanical properties. Although research indicates AlSi10Mg parts produced by selective laser melting have characteristically very fine microstructures, there is a need for more intensive study to comprehend the effect of heat treatment on the mechanical properties of this alloy by analyzing its microstructure. In this study, AlSi10Mg specimens heat-treated at varying temperatures were analyzed by optical and electron microscopes. Micro-indentation hardness and tensile tests were performed to evaluate mechanical properties while considering the specimen build orientation. Observation shows that it is nearly impossible to completely dissolve the evolved second phase silicon-rich particles, which may have significant effects on the mechanical characteristics. Electron microscopy images show the evolution of iron-rich particles in the Al matrix, which may have a significant influence on the mechanical properties of the alloy.
Uncertainties in Liner Shipping and Ship Schedule Recovery: A State-of-the-Art Review
Authors: Elmi Zeinab , Singh Prashant , Meriga Vamshi Krishna , Goniewicz Krzysztof , Borowska-Stefańska Marta , Wiśniewski Szymon , Dulebenets Maxim A.
Published: April 2022

Each shipping line is expected to establish a reliable operating model, and the design of ship schedules is a key operational consideration. Long-term profits for shipping lines can be expected from a well-designed ship schedule. In today’s liner service design, managing the time factor is critical. Shipping schedules are prone to different unexpected disruptions. Such disruptions would necessitate a near-real-time analysis of port capacity and re-design of the original ship schedule to offset the negative externalities. Ship schedule recovery strategies should be implemented to mitigate the effects caused by disruptions at ports or at sea, which may include, but are not limited to, ship sailing speed adjustment, handling rate adjustment at ports, port skipping, and port skipping with container diversion. A proper selection of ship schedule recovery strategies is expected to minimize deviations from the original ship schedule and reduce delays in the delivery of cargoes to the destination ports. This article offers a thorough review of the current liner shipping research primarily focusing on two major themes: (1) uncertainties in liner shipping operations; and (2) ship schedule recovery in response to disruptive events. On the basis of a detailed review of the available literature, the obtained results are carefully investigated, and limitations in the current state-of-the-art are determined for every group of studies. Furthermore, representative mathematical models are provided that could be further used in future research efforts dealing with uncertainties in liner shipping and ship schedule recovery. Last but not least, a few prospective research avenues are suggested for further investigation.
Experimental and Numerical Study on the Characteristics of Motion and Load for a Floating Solar Power Farm under Regular Waves
Authors: Lee Jun-Hee , Paik Kwang-Jun , Lee Soon-Hyun , Hwangbo Jun , Ha Tae-Hyu
Published: April 2022

Recently, the demand for floating solar power farms in lakes and coasts (rather than on land) has been increasing rapidly. It is important to develop a numerical analysis technique that considers environmental conditions to predict structural stability and accurate motion response while designing a floating solar power farm. In this study, we performed a comparison under conditions similar to those of the Inha University towing tank (IUTT) model test to verify the numerical analysis method. The results revealed that heave and pitch movements were dominant under head sea conditions. Relative behavior occurred because of the hinge connection of each unit, and complex motion characteristics appeared depending on the wave conditions. The numerical method was verified based on the motion response and load of the floating solar farm. The validity of the results was also confirmed.
An Energy-Saving Position Control Strategy for Deep-Sea Valve-Controlled Hydraulic Cylinder Systems
Authors: Wu Jia-Bin , Li Li , Yan Yong-Kang , Wang Pin-Jian , Wei Wei
Published: April 2022

The valve-controlled hydraulic cylinder system (VCHCS) is commonly utilized in the underwater manipulator, which is the most important tool for subsea tasks. Hydraulic oil viscosity is very sensitive to pressure. Therefore, when working at different depths under different ambient pressures in the sea, the hydraulic oil viscosity and the pipeline pressure loss in the deep-sea VCHCS vary greatly, which seriously affects the energy efficiency of the system. In addition, the control accuracy of the deep-sea VCHCS is also influenced by changes in the hydraulic oil viscosity and the pipeline pressure loss. In order to realize energy-saving control, this research introduces a proportional relief valve and develops a variable pump pressure control strategy. At the same time, a variable gain proportional-integral-derivative (PID) algorithm is designed to achieve precise control. A co-simulation model of the deep-sea VCHCS is then established, and many simulation analyses are carried out. Compared with traditional PID control with a constant pump pressure, the proposed method presents advantages such as lower energy consumption, better control accuracy, better resistance to load impact, and accuracy consistency under different working depths. Among them, when working at 11 km depth in the sea, the proposed method is capable of saving energy by 36.5% for the multi-step movement, by 30% for the harmonic movement, and by 47% for the complex movement. The present work in this research provides a solution that can realize energy saving and precise control of the deep-sea VCHCS at the same time in the wide span of depth in the sea.
Research on Ship Trajectory Classification Based on a Deep Convolutional Neural Network
Authors: Guo Tao , Xie Lei
Published: April 2022

With the aim of solving the problems of ship trajectory classification and channel identification, a ship trajectory classification method based on deep a convolutional neural network is proposed. First, the ship trajectory data are preprocessed using the improved QuickBundle clustering algorithm. Then, data are converted into ship trajectory image data, a dataset is established, a deep convolutional neural network-based ship trajectory classification model is constructed, and the manually annotated dataset is used for training. The fully connected neural network model and SVM model with latitude and longitude data as input are selected for comparative analysis. The results show that the ship trajectory classification model based on a deep convolutional neural network can effectively distinguish ship trajectories in different waterways, and the proposed method is an effective ship trajectory classification method.
Evidence of Former Sea Levels from a Passive Seismic Survey at a Sandy Beach; Perranporth, SW England, UK
Authors: Payo Andres , Jenkins Gareth O. , Morgan Dave , Valiente Nieves G. , Scott Timothy
Published: April 2022

Since the end of the last glaciation, the United Kingdom’s land surface has been altered by isostatic rebound, rising in the north and sinking in the south. Numerous studies have been published documenting the impact of isostatic rebound on relative sea levels. However, due to the difficulties in acquiring evidence to prove former sea levels, locally, these data can be sparse or absent. In this work, we explored the suitability of the passive seismic survey (PSS) method to estimate the contemporaneous beach thickness in coastal environments where there is a high impedance contrast between the beach deposits and the underlying wave-cut platform. We conducted a three-day survey at Perran Beach, Cornwall, collected 149 measurements using PSS, and interpreted the observations supported by auxiliary topographical, geological, and independent geophysical observation in the study area. The study site is a contemporaneous beach mostly composed of sand underlain by a wave-cut platform composed of igneous and sedimentary rock, therefore high impedance contrast with the sandy beach is anticipated. The elevation of the bedrock relative to the topographical elevation suggests that the bedrock elevation is −15 m ± 5 m below the present day mean sea level, which is coherent with the observation of relative sea level rise along the region of the south-west. The present study contributes to our current limited understanding of land and sea level movements by providing further subsurface information to the coastal geological archive of south-west England, a region currently in need of more data to reconstruct land- and sea-level movements.
Emerging Downdrift Erosion by Twin Long-Range Jetties on an Open Mesotidal Muddy Coast, China
Authors: Wang Yu-Hai , Wang Yan-Hong , Deng An-Jun , Feng Hao-Chuan , Wang Dang-Wei , Guo Chuan-Sheng
Published: April 2022

Downdrift shoreline recession associated with the construction of a shore-crossing hard structure represents one of coastal erosional hotspots that must be addressed for an integrated, sustainable coastal zone management. To prevent siltation within the navigation channel, two rubber-mounted jetties were installed at the Sheyang River mouth on the open mesotidal muddy coast in Jiangsu province, China, in October 2013. The north jetty is 7.9 km long, while the south jetty is 7.8 km long. The net longshore sediment transport is from the north to the south due to flood-tide dominance. As disclosed by high-resolution satellite images, a 36-km-long downdrift shoreline stretch had experienced remarkable retreats at alongshore varying rates by March 2019. The eroding shoreline planform does not resemble a classic “S” shape, a crescentic shape, or a parabolic shape but an irregularly indented curved shape. Transect topographic survey also reveals an almost immediate response of the downdrift coast from the original accretionary scenery to an erosional regime, with the erosion front translocating downcoast at a much faster speed than a normal speed of 1–1.5 km/yr. Using FVCOM and SWAN, 2DH process-based numerical simulations are performed to simulate the flow, the sediment transport, and the yearly-magnitude accretion/erosion distribution in the jetty-affected area by a representative tidal force and an annual-magnitude wave force. The results demonstrate that the reciprocal tidal flow is predominantly responsible for the muddy sediment accretions at downdrift intertidal and surf zones shallower than a 4.0-m isobath, whereas big wind waves play a decisive role in triggering and developing the downdrift erosional process. The predicted spatial extent of the downdrift erosional segment matches closely the actual eroding front. The loss of the net annual longshore sediment transport volume, i.e., 3.08 million m3 due to the blockage by the twin jetties is recovered from a much larger spatial extent than the 36-km-long retreating shoreline stretch. With regard to the Bruun model, the one-line model, the headland-bay model, and the 2DH numerical model, the potential maximum recession length and the planform shape of the downdrift erosional shoreline arc are further elaborated to gain new insights into the spatial and temporal impact of a hard structure on the adjacent shoreline and flat (beach).
Surge Response Analysis of the Serbuoys-TLP Tension Leg Platform under the Action of Wave–Current Coupling
Authors: Ma Zhe , Lou Zhehan , Zhai Gangjun , Li Jiakang
Published: April 2022

A new type of tension leg platform (TLP) connected to a series of buoys (Serbuoys-TLP) has been proven to effectively suppress the surge response of the platform during wave conditions. However, in the complex marine environment, it is more relevant to study its motion response to the action of waves and currents. Considering the tension tendon as a lumped mass model, a DUTMST 2.0 time-domain simulation program was written, based on MATLAB, which can accurately calculate the surge response of the Serbuoys-TLP under wave–current coupling conditions. The suppression efficiency of the Serbuoys-TLP on the surge response was analyzed under different current velocities and wave parameters, and the results showed that the suppression efficiency by the Serbuoys-TLP of surges was higher under the action of waves and currents compared with the action of waves. In addition, the surge response of the platform under the two conditions of wave–current combination and wave–current coupling was also investigated, where wave–current coupling considers the effect of the current’s velocity on the wave period, while the wave–current combination does not consider it, which means that the wave and current are linearly superimposed. The results show that the surge response of the platform will be overestimated without considering the coupling effect of waves and currents. The effect of wave–current coupling has a greater impact on the surge response of the Serbuoys-TLP than that of conventional TLP. Therefore, in the design of new floating structures, the motion performance in response to the effect of wave–current coupling should be paid full attention.
A Novel Sound Speed Profile Prediction Method Based on the Convolutional Long-Short Term Memory Network
Authors: Li Bingyang , Zhai Jingsheng
Published: April 2022

As an important marine environmental parameter, sound velocity greatly affects the sound propagation characteristics in the ocean. In marine surveying work, prompt and low-cost acquisition of accurate sound speed profiles (SSP) is of immense significance for improving the measurement and positioning accuracy of marine acoustic equipment and ensuring underwater wireless communication. To address the problem of not being able to glean the accurate SSP in real time, we propose a convolution long short-term memory neural network (Conv-LSTM) which combines the long short-term memory (LSTM) neural network and convolution operation to predict the complete sound speed profile based on historical data. Considering SSP is a typical time series and has strong spatial correlation, Conv-LSTM can grasp not only the temporal relevance of time series, but also the spatial characteristics. The Argo temperature and salinity grid data of the North Pacific from 2004 to 2019 is imported to establish the model’s SSP dataset, and the convolution of input data is performed before going through the neurons in this recurrent neural network to extract the spatial relevance of the data itself. In the meantime, in order to prove the advanced nature of this model, we compare it with the LSTM network under the same parameter settings. The experimental results show that predicting the SSP time series at a single coordinate position under the same parameter conditions, it is best to predict the future SSP next month through the historical data of 24 months, and the prediction effect of Conv-LSTM is much better than that of the LSTM network, and the relative error (RE) is 0.872 m/s, which is 1.817 m/s less than that of LSTM. Predictions in the selected area are also exceedingly accurate relative to the actual data; the prediction error of deep water is less than 0.3 m/s, while RE on the surface layer is larger, exceeding 1.6 m/s.
Oceanic Internal Waves and Internal Tides in the East Asian Marginal Seas
Authors: Nam Sunghyun , Chen Xueen
Published: April 2022

Inertia-gravity waves or internal waves (IWs) are ubiquitous in the stratified, rotating ocean [...]
Shipping Decarbonization: An Overview of the Different Stern Hydrodynamic Energy Saving Devices
Authors: Spinelli Flaminia , Mancini Simone , Vitiello Luigi , Bilandi Rasul Niazmand , De Carlini Maria
Published: April 2022

In recent years, research into ships has focused on reducing emissions, consuming less energy, and being more efficient. As a result, the maritime industry has been continuing in a green and sustainable direction. Improving the fuel efficiency of ships and the decarbonization of shipping are important issues to reduce fuel consumption and emitted Greenhouse Gas (GHG) amounts. Decarbonization in the shipping industry could be achieved through technical and operational strategies such as Energy Saving Devices (ESDs) to reduce the fuel consumption of new and existing ships. According to the makers, ESDs can optimize fuel efficiency by up to 15%. This paper reviews the current literature on stern hydrodynamic ESDs, which are mainly used on typical merchant vessels, i.e., bulkers, tankers, and carriers. A comprehensive review is carried out analysing the different available solutions for stern hydrodynamic ESDs, the working principles, the methods used for the design, optimization, and evaluation of the performance improvements, and the relevant issues of these specific ESDs.
Promising Antifungal Activity of Encephalartos laurentianus de Wild against Candida albicans Clinical Isolates: In Vitro and In Vivo Effects on Renal Cortex of Adult Albino Rats
Authors: Negm Walaa A. , El-Aasr Mona , Attia Ghada , Alqahtani Moneerah J. , Yassien Rania Ibrahim , Abo Kamer Amal , Elekhnawy Engy
Published: April 2022

Candida albicans can cause various infections, especially in immunocompromised patients. Its ability to develop resistance to the current antifungal drugs as well as its multiple virulence factors have rendered the problem even more complicated. Thus, in the present investigation, we elucidated an in vitro and in vivo antifungal activity of Encephalartos laurentianus methanol extract (ELME) against C. albicans clinical isolates for the first time. A phytochemical identification of 64 compounds was conducted in ELME using LC-MS/MS. Interestingly, ELME exhibited antifungal activity with MIC values that ranged from 32–256 µg/mL. Furthermore, we investigated the antibiofilm activity of ELME against the biofilms formed by C. albicans isolates. ELME displayed antibiofilm activity using a crystal violet assay as it decreased the percentages of cells, moderately and strongly forming biofilms from 62.5% to 25%. Moreover, the antibiofilm impact of ELME was elucidated using SEM and fluorescent microscope. A significant reduction in the biofilm formation by C. albicans isolates was observed. In addition, we observed that ELME resulted in the downregulation of the biofilm-related tested genes (ALS1, BCR1, PLB2, and SAP5) in 37.5% of the isolates using qRT-PCR. Besides, the in vivo antifungal activity of ELME on the kidney tissues of rats infected with C. albicans was investigated using histological and immunohistochemical studies. ELME was found to protect against C. albicans induced renal damage, decrease desmin and inducible nitric oxide synthase, increase alkaline phosphatase, and increase infected rats’ survival rate. Additionally, the cytotoxicity of ELME was elucidated on Human Skin Fibroblast normal cells using MTT assay. ELME had an IC50 of 31.26 µg/mL. Thus, we can conclude that ELME might be a promising future source for antifungal compounds.
Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast Saccharomyces cerevisiae
Authors: Jiang Wei , Li Chao , Li Yanjun , Peng Huadong
Published: April 2022

Microbial lipids have been a hot topic in the field of metabolic engineering and synthetic biology due to their increased market and important applications in biofuels, oleochemicals, cosmetics, etc. This review first compares the popular hosts for lipid production and explains the four modules for lipid synthesis in yeast, including the fatty acid biosynthesis module, lipid accumulation module, lipid sequestration module, and fatty acid modification module. This is followed by a summary of metabolic engineering strategies that could be used for enhancing each module for lipid production. In addition, the efforts being invested in improving the production of value-added fatty acids in engineered yeast, such as cyclopropane fatty acid, ricinoleic acid, gamma linoleic acid, EPA, and DHA, are included. A discussion is further made on the potential relationships between lipid pathway engineering and consequential changes in cellular physiological properties, such as cell membrane integrity, intracellular reactive oxygen species level, and mitochondrial membrane potential. Finally, with the rapid development of synthetic biology tools, such as CRISPR genome editing tools and machine learning models, this review proposes some future trends that could be employed to engineer yeast with enhanced intracellular lipid production while not compromising much of its cellular health.
A Phylogenetic and Taxonomic Study on Phellodon (Bankeraceae, Thelephorales) from China
Authors: Song Chang-Ge , Chen Yuan-Yuan , Liu Shun , Xu Tai-Min , He Xiao-Lan , Wang Di , Cui Bao-Kai
Published: April 2022

In this study, phylogenetic analyses of Phellodon from China were carried out based on sequences from the internal transcribed spacer (ITS) regions, the large subunit of nuclear ribosomal RNA gene (nLSU), the small subunit of nuclear ribosomal RNA gene (nSSU), the largest subunit of RNA polymerase II (RPB1), and the second largest subunit of RNA polymerase II (RPB2), combined with morphological characters of the collected specimens in China. The fruiting bodies of the specimens were used to observe their characteristics, and three new species of Phellodon are discovered. Phellodon crassipileatus is characterized by its pale brown to dark brown pileal surface, tomentose pileal margin, white spines, and the presence of clamp connections in generative hyphae of pileal surface, context, and stipe. Phellodon griseofuscus is characterized by its dark brown to black pileal surface, white to pale brown pileal margin, the presence of both simple septa and clamp connections in generative hyphae of spines, and moderately long basidia. Phellodon perchocolatus is characterized by its woody and broad pileus, brown to greyish brown pileal surface when fresh, tomentose pileal margin when young, which becomes glabrous with age, and the presence of both simple septa and clamp connections in the generative hyphae of the spines. This is the first time both single and multi-genes analysis is used in such a phylogenetic and taxonomic study on Phellodon, which can provide the basis for the phylogenetic study of the genus.
Environmental Factors Affecting Diversity, Structure, and Temporal Variation of Airborne Fungal Communities in a Research and Teaching Building of Tianjin University, China
Authors: Lu Yixuan , Wang Xiao , Almeida Lucineidy C. S. de S. , Pecoraro Lorenzo
Published: April 2022

Airborne fungi are widely distributed in the environment and may have adverse effects on human health. A 12-month survey on the diversity and concentration of culturable airborne fungi was carried out in a research and teaching building of Tianjin University. Indoor and outdoor environments were analyzed using an HAS-100B air sampler. A total of 667 fungal strains, belonging to 160 species and 73 genera were isolated and identified based on morphological and molecular analysis. The most abundant fungal genera were Alternaria (38.57%), Cladosporium (21.49%), and Aspergillus (5.34%), while the most frequently appearing species was A. alternata (21%), followed by A. tenuissima (12.4%), and C. cladosporioides (9.3%). The concentration of fungi in different environments ranged from 0 to 150 CFU/m3 and was significantly higher outdoor than indoor. Temperature and sampling month were significant factors influencing the whole building fungal community, while relative humidity and wind speed were highly correlated with fungal composition outdoor. Variations in the relative abundance of major airborne fungal taxa at different heights above-ground could lead to different community structures at different floors. Our results may provide valuable information for air quality monitoring and microbial pollution control in university building environments.
Yeast Trk1 Potassium Transporter Gradually Changes Its Affinity in Response to Both External and Internal Signals
Authors: Masaryk Jakub , Sychrová Hana
Published: April 2022

Yeasts need a high intracellular concentration of potassium to grow. The main K+ uptake system in Saccharomyces cerevisiae is the Trk1 transporter, a complex protein with four MPM helical membrane motifs. Trk1 has been shown to exist in low- or high-affinity modes, which reflect the availability of potassium in the environment. However, when and how the affinity changes, and whether the potassium availability is the only signal for the affinity switch, remains unknown. Here, we characterize the Trk1 kinetic parameters under various conditions and find that Trk1’s KT and Vmax change gradually. This gliding adjustment is rapid and precisely reflects the changes in the intracellular potassium content and membrane potential. A detailed characterization of the specific mutations in the P-helices of the MPM segments reveals that the presence of proline in the P-helix of the second and third MPM domain (F820P and L949P) does not affect the function of Trk1 in general, but rather specifically prevents the transporter’s transition to a high-affinity state. The analogous mutations in the two remaining MPM domains (L81P and L1115P) result in a mislocalized and inactive protein, highlighting the importance of the first and fourth P-helices in proper Trk1 folding and activity at the plasma membrane.
Growth and Yield of Purple Kculli Corn Plants under Different Fertilization Schemes
Authors: Romero-Cortes Teresa , Tamayo-Rivera Lis , Morales-Ovando Mario A. , Aparicio Burgos José E. , Pérez España Victor H. , Peralta-Gil Martin , Cuervo-Parra Jaime A.
Published: April 2022

Globally, corn is the most economically important crop, surpassing other cereals of economic importance. However, the tillage methods, monoculture and the abuse of synthetic agrochemicals used in Mexico have led to the loss of fertility and soil yield. In this sense, the application of alternative fertilization methods based on chemical fertilizer, organic matter and biofertilizer, applied alone or in combination, can stimulate the defense systems of corn plants and increase their yield. Therefore, in this research, some fertilization schemes were tested on purple corn plants of the Kculli race through the evaluation of some growth and yield variables, as well as the subsequent evaluation of the chemical characteristics of the corn grain produced in each fertilization scheme. The results indicate highly significant differences (p ≤ 0.05) between treatments, for the different growth and yield variables studied. Of all the fertilization schemes evaluated, treatment T7 obtained the best grain yield of 6.19 ± 0.07 t ha−1, with respect to treatment T1 of 1.02 ± 0.01 t ha−1, as well as the highest protein content and starch quality. Being clear the positive effect of the adequate contribution of the macro and micronutrients used exerts on the corn crop in each of the fertilization schemes studied. On the other hand, the analysis carried out on the grains was found within the values reported by other authors.
Cell Wall Integrity and Its Industrial Applications in Filamentous Fungi
Authors: Yoshimi Akira , Miyazawa Ken , Kawauchi Moriyuki , Abe Keietsu
Published: April 2022

Signal transduction pathways regulating cell wall integrity (CWI) in filamentous fungi have been studied taking into account findings in budding yeast, and much knowledge has been accumulated in recent years. Given that the cell wall is essential for viability in fungi, its architecture has been analyzed in relation to virulence, especially in filamentous fungal pathogens of plants and humans. Although research on CWI signaling in individual fungal species has progressed, an integrated understanding of CWI signaling in diverse fungi has not yet been achieved. For example, the variety of sensor proteins and their functional differences among different fungal species have been described, but the understanding of their general and species-specific biological functions is limited. Our long-term research interest is CWI signaling in filamentous fungi. Here, we outline CWI signaling in these fungi, from sensor proteins required for the recognition of environmental changes to the regulation of cell wall polysaccharide synthesis genes. We discuss the similarities and differences between the functions of CWI signaling factors in filamentous fungi and in budding yeast. We also describe the latest findings on industrial applications, including those derived from studies on CWI signaling: the development of antifungal agents and the development of highly productive strains of filamentous fungi with modified cell surface characteristics by controlling cell wall biogenesis.
Keratinocyte Response to Infection with Sporothrix schenckii
Authors: Paredes-Rojas Araceli , Palma-Ramos Alejandro , Castrillón-Rivera Laura Estela , Mendoza-Pérez Felipe , Navarro-González María del Carmen , Arenas-Guzmán Roberto , Castañeda-Sánchez Jorge Ismael , Luna-Herrera Julieta
Published: April 2022

Sporotrichosis is a subacute, or chronic mycosis caused by traumatic inoculation of material contaminated with the fungus Sporothrix schenckii which is part of the Sporothrix spp. complex. The infection is limited to the skin, although its progression to more severe systemic or disseminated forms remains possible. Skin is the tissue that comes into contact with Sporothrix first, and the role of various cell lines has been described with regard to infection control. However, there is little information on the response of keratinocytes. In this study, we used the human keratinocyte cell line (HaCaT) and evaluated different aspects of infection from modifications in the cytoskeleton to the expression of molecules of the innate response during infection with conidia and yeast cells of Sporothrix schenckii. We found that during infection with both phases of the fungus, alterations of the actin cytoskeleton, formation of membrane protuberances, and loss of stress fibers were induced. We also observed an overexpression of the surface receptors MR, TLR6, CR3 and TLR2. Cytokine analysis showed that both phases of the fungus induced the production of elevated levels of the chemokines MCP-1 and IL-8, and proinflammatory cytokines IFN-α, IFN-γ and IL-6. In contrast, TNF-α production was significant only with conidial infection. In late post-infection, cytokine production was observed with immunoregulatory activity, IL-10, and growth factors, G-CSF and GM-CSF. In conclusion, infection of keratinocytes with conidia and yeast cells of Sporothrix schenckii induces an inflammatory response and rearrangements of the cytoskeleton.
Frequency and Duration of, and Risk Factors for, Diagnostic Delays Associated with Histoplasmosis
Authors: Miller Aaron C. , Arakkal Alan T. , Koeneman Scott H. , Cavanaugh Joseph E. , Thompson George R. , Baddley John W. , Polgreen Philip M.
Published: April 2022

Histoplasmosis is often confused with other diseases leading to diagnostic delays. We estimated the incidence, length of, and risk factors for, diagnostic delays associated with histoplasmosis. Using data from IBM Marketscan, 2001–2017, we found all patients with a histoplasmosis diagnosis. We calculated the number of visits that occurred prior to the histoplasmosis diagnosis and the number of visits with symptomatically similar diagnoses (SSDs). Next, we estimated the number of visits that represented a delay using a simulation-based approach. We also computed the number of potential opportunities for diagnosis that were missed for each patient and the length of time between the first opportunity and the diagnosis. Finally, we identified risk factors for diagnostic delays using a logistic regression model. The number of SSD-related visits increased significantly in the 97 days prior to the histoplasmosis diagnosis. During this period, 97.4% of patients had a visit, and 90.1% had at least one SSD visit. We estimate that 82.9% of patients with histoplasmosis experienced at least one missed diagnostic opportunity. The average delay was 39.5 days with an average of 4.0 missed opportunities. Risk factors for diagnostic delays included prior antibiotic use, history of other pulmonary diseases, and emergency department and outpatient visits, especially during weekends. New diagnostic approaches for histoplasmosis are needed.
The Feasibility of Ibrexafungerp for the Treatment of Fungal Infections in Patients with Hematological Malignancies
Authors: Daraskevicius Justinas , Petraitis Vidmantas , Davainis Linas , Zucenka Andrius
Published: April 2022

Invasive fungal diseases (IFD) remain a major cause of morbidity and mortality in hematological patients, especially those undergoing hematopoietic stem cell transplantation (HSCT). Despite relatively high incidence, diagnosis and treatment remain a challenge due to non-specific manifestation and limited antifungal armamentarium. A first-in-class triterpenoid antifungal ibrexafungerp that acts by inhibiting the glucan synthase enzyme in the fungal cell wall was recently approved by the USA Food and Drug Administration for the treatment of vaginal yeast infections. Preclinical data show activity against numerous fungi species, including azole- and echinocandin-resistant strains. Preliminary data from ongoing phase 3 studies in IFD have been encouraging, but the role of ibrexafungerp in hematological patients who develop fungal infections has not yet been described. Herein, we discuss the feasibility of oral ibrexafungerp-based antifungal therapy for adult patients with hematological malignancies who have either undergone HSCT or received treatment with a novel targeted therapy agent. We present four clinical cases where ibrexafungerp alone or in combination with other antifungal agents was successfully employed for the management of refractory fungal infection. We describe real-life experiences showing the potential clinical implementation of ibrexafungerp for patients with hematological malignancies for the first time, and provoke future discussion.
Technical Performance of a 430-Gene Preventative Genomics Assay to Identify Multiple Variant Types Associated with Adult-Onset Monogenic Conditions, Susceptibility Loci, and Pharmacogenetic Insights
Authors: Silver Ari , Lazarin Gabriel A. , Silver Maxwell , Miller Meghan , Jansen Michael , Wechsberg Christine , Dekanek Erin , Grossfeld Stav , Herpel Tim , Gunatilake Dinura , Bisignano Alexander , Jaremko Malgorzata
Published: April 2022

DNA-based screening in individuals without known risk factors potentially identifies those who may benefit from genetic counseling, early medical interventions, and/or avoidance of late or missed diagnoses. While not currently in widespread usage, technological advances in genetic analysis overcome barriers to access by enabling less labor-intensive and more cost-efficient means to discover variants of clinical importance. This study describes the technical validation of a 430-gene next-generation sequencing based assay, GeneCompassTM, indicated for the screening of healthy individuals in the areas of actionable health risks, pharmaceutical drug response, and wellness traits. The test includes genes associated with Mendelian disorders and genetic susceptibility loci, encompassing 14 clinical areas and pharmacogenetic variants. The custom-designed target enrichment capture and bioinformatics pipelines interrogate multiple variant types, including single nucleotide variants, insertions/deletions (indels), copy number variants, and functional haplotypes (star alleles), including tandem alleles and structural variants. Validation was performed against reference DNA from three sources: 1000 Genomes Project (n = 3), Coriell biobank (n = 105), and previously molecularly characterized biological specimens: blood (n = 15) and saliva (n = 11). Analytical sensitivity and specificity for single nucleotide variants (SNVs) were 97.57% and 99.99%, respectively, and for indels were 74.57% and 97.34%, respectively. This study demonstrates the validity of an NGS assay for genetic screening and the broadening of access to preventative genomics.
Adaptive Radiotherapy in Head and Neck Cancer Using Volumetric Modulated Arc Therapy
Authors: Buciuman Nikolett , Marcu Loredana G.
Published: April 2022

A dosimetric study was performed to show the importance of adaptive radiotherapy (ART) for head and neck cancer (HNC) patients using volumetric modulated arc therapy (VMAT). A total of 13 patients with HNC who required replanning during radiotherapy were included in this study. All plans succeeded to achieve the set objectives regarding target volume coverage and organ sparing. All target volumes presented a significant decrease with an average of 76.44 cm3 (p = 0.007) for PTVlow risk, 102.81 cm3 (p = 0.021) for PTVintermediate risk, and 47.10 cm3 (p = 0.003) for PTVhigh risk. Additionally, a positive correlation was found between PTV shrinkage and the number of fractions completed before replanning. Significant volume decrease was also observed for the parotid glands. The ipsilateral parotid decreased in volume by a mean of 3.75 cm3 (14.43%) (p = 0.067), while the contralateral decreased by 4.23 cm3 (13.23%) (p = 0.033). For all analyzed organs, a reduction in the final dose received after replanning was found. Our study showed that ART via rescanning, recontouring, and replanning using VMAT is essential whenever anatomical and positional variations occur. Furthermore, comparison with the literature has confirmed that ART using VMAT offers similar results to ART with intensity modulated radiotherapy.
A State-of-the-Art Roadmap for Biomarker-Driven Drug Development in the Era of Personalized Therapies
Authors: Serelli-Lee Victoria , Ito Kazumi , Koibuchi Akira , Tanigawa Takahiko , Ueno Takayo , Matsushima Nobuko , Imai Yasuhiko
Published: April 2022

Advances in biotechnology have enabled us to assay human tissue and cells to a depth and resolution that was never possible before, redefining what we know as the “biomarker”, and how we define a “disease”. This comes along with the shift of focus from a “one-drug-fits-all” to a “personalized approach”, placing the drug development industry in a highly dynamic landscape, having to navigate such disruptive trends. In response to this, innovative clinical trial designs have been key in realizing biomarker-driven drug development. Regulatory approvals of cancer genome sequencing panels and associated targeted therapies has brought personalized medicines to the clinic. Increasing availability of sophisticated biotechnologies such as next-generation sequencing (NGS) has also led to a massive outflux of real-world genomic data. This review summarizes the current state of biomarker-driven drug development and highlights examples showing the utility and importance of the application of real-world data in the process. We also propose that all stakeholders in drug development should (1) be conscious of and efficiently utilize real-world evidence and (2) re-vamp the way the industry approaches drug development in this era of personalized medicines.
Renoprotective Effect of Vardenafil and Avanafil in Contrast-Induced Nephropathy: Emerging Evidence from an Animal Model
Authors: Zisis Ioannis-Erineos , Georgiadis Georgios , Docea Anca Oana , Calina Daniela , Cercelaru Liliana , Tsiaoussis John , Lazopoulos Georgios , Sofikitis Nikolaos , Tsatsakis Aristidis , Mamoulakis Charalampos
Published: April 2022

The potential renoprotective effects of vardenafil (VAR) have been evaluated in a very limited number of studies using acute kidney injury animal models other than contrast-induced nephropathy (CIN) with promising results, while avanafil (AVA) has not been evaluated in this respect before. The purpose of this study was to evaluate for the first time the potential renoprotective effect of VAR and AVA in a rat model of CIN. Twenty-five male Wistar rats were equally assigned into five groups: control, CIN, CIN+N-acetyl cysteine (NAC) (100 mg/kg/day) as a positive control, CIN+VAR (10 mg/kg/day) and CIN+AVA (50 mg/kg/day). CIN was induced by dehydration, inhibition of prostaglandin and nitric oxide synthesis as well as exposure to the contrast medium (CM). Serum Cr (sCr) levels were measured at 24 and 48 h after CIN induction. At 48 h of CM exposure, animals were sacrificed. Matrix metalloproteinase (MMP) 2 (MMP-2) and MMP-9, kidney injury molecule 1 (KIM-1) and cystatin-C (Cys-C) were measured on renal tissue. Histopathological findings were evaluated on kidney tissue. All treatment groups had close to normal kidney appearance. sCr levels subsided in all treatment groups compared to CIN group at 48 h following CIN induction. A significant decline in the levels of MMP-2, MMP-9, KIM-1 and Cys-C compared to CIN group was observed. These results provide emerging evidence that VAR and AVA may have the potential to prevent CIN.
Early Intervention in Orbital Floor Fractures: Postoperative Ocular Motility and Diplopia Outcomes
Authors: Hsu Cherng-Ru , Lee Lung-Chi , Chen Yi-Hao , Chien Ke-Hung
Published: April 2022

Purpose: Orbital floor fractures commonly occur during orbital trauma. Currently, the indications for orbital fracture repair and the appropriate duration between trauma and surgical intervention remain controversial. Methods: Eyes diagnosed with orbital floor fractures that underwent reconstruction surgery were retrospectively reviewed. Demographic data were analyzed. Patients were classified based on the timing of the surgical intervention after injury. Ocular limitation and diplopia were evaluated preoperatively and postoperatively at one week, one month, and three months. Results: Two hundred seventy eyes of 270 patients (174 males and 96 females, mean age: 40.9 ± 16.3 years) were identified. The mean duration from injury to surgical intervention was 18.0 ± 21.2 days (range: 0–117 days). In the subgroup analysis, compared to delayed treatment, the early repair of floor fractures, i.e., within 7 days, was associated with significant motility and diplopia resolution at one week (p = 0.001, p < 0.001), one month (p < 0.001, p < 0.001), and three months (p < 0.001, p < 0.001). Sex and the duration from injury to repair were significantly associated with postoperative ocular motility (p = 0.001; p = 0.024) and diplopia (p < 0.001; p = 0.008) at three months. Multivariate analysis revealed that preoperative limitation and diplopia were correlated with postoperative limitation (p = 0.007) and diplopia (p = 0.001), respectively. Conclusions: The duration between orbital floor fracture and surgical treatment was associated with postoperative limitation and diplopia. Our results suggest that earlier intervention in symptomatic patients with orbital trauma may improve postoperative visual function.