bluuebunny/crossref_metadata_2025 huggingface dataset:
PSXV-21 Assessment of the presence of anthelmintic resistance in Maryland beef cattle herds
Authors: Potts Sarah B , Grev Amanda M , Semler Jeffery W , Schoenian Susan , O’Brien Dahlia
Published: October 2021

Reduced anthelmintic efficacy in beef cattle has recently been documented at the national level. The objective of this study was to determine if anthelmintic resistance is present in Maryland beef cattle herds. For this study, the fecal egg count reduction (FECR) test was used to assess anthelmintic efficacy of composite samples. Between July and October 2020, anthelmintic efficacy was assessed for 17 groups of cattle across 14 individual farms in Maryland. Each test group consisted of 24 ± 6 calves or yearlings (< 16 months of age). Previous anthelmintic treatment history was used to assign each group to one of four commercially available anthelmintics: moxidectin (injectable), moxidectin (pour-on), doramectin, or fenbendazole. Prior to treatment, cattle were weighed to ensure appropriate dose according to the product label. Fecal samples were collected from all cattle within each group before and after (17 ± 5 d) treatment. Pre- and post-treatment samples were composited for each group on an equal-weight basis (10g per animal) and the number of eggs within the sample was determined using the 3-chambered McMaster technique. The mean FECR across all four anthelmintics was 60 ± 19% (mean ± 95% confidence interval), indicating that the anthelmintics tested in this study were largely ineffective. The FECR for the moxidectin injectable, moxidectin pour-on, doramectin, and fenbendazole treatments were 71 ± 38%, 47 ± 46%, 35 ± 88%, and 96 ± 18% (mean ± 95% confidence interval), respectively. Although these data indicate no statistically significant differences for efficacy among the four products tested, they do suggest that efficacy is highly variable across different anthelmintics and farms. Results also indicate that anthelmintic resistance exists on many cattle farms in Maryland. This study provides evidence that supports the need for more judicious anthelmintic use and alternative gastrointestinal parasite control measures in Maryland beef cattle herds.
PSXVI-11 Reference intervals of concentrations of essential and toxic elements in the hair of Kalmyk cows bred in the Republic of Sakha (Yakutia) of Russia, calculated by the AES-ICP and MS-ICP methods
Authors: Okhlopkov Sargylaan , Martynov Andrey , Vasiliev Yakov , Mironova Irina , Khabibullin Ruzel’ , Rakhmatullin Rafael’
Published: October 2021

Reference intervals of concentrations of chemical elements in biological substrates of animals and humans are the main tool for interpreting the results of laboratory studies. However, establishing the reference intervals, it is necessary to take into account the factor of regional characteristics that consist of a number of elements: climate, terrain, the degree of anthropogenic loads, etc. In this regard, the main purpose of this study was to establish reference intervals for the concentration of chemical elements in the hair of cows of the Kalmyk breed bred in a separate biogeochemical province. The studies were carried out on cows of the Kalmyk breed (n = 126; age 3–5 years) bred in the Republic of Sakha (Yakutia) of Russia. Hair samples were taken with the upper withers of an animal. The elemental composition of the samples was estimated according to 25 chemical elements by ICP-AES and ICP-MS. Reference intervals for the content of chemical elements in animal hair were calculated according to the IUPAC recommendations (Poulsen OM et al., 1997) (2.5–97.5 percentile). As a result of the research, the following values of the boundaries of intervals have been established: Al (139–704); As (0.051–0.287); B (0.921–4.95); Ca (1272–3391); Cd (0.002–0.014); Co (0.111–0.517); Cr (0.674–3.09); Cu (4,840–8.99); Fe (250–1120); Hg (0.004–0.052); I (0.266–1.61); K (1433–6687); Li (0.248–0.857); Mg (393–2252); Mn (7.22–65.89); Na (1013–5258); Ni (0.448–2.88); P (160.0–374); Pb (0.170–0.615); Se (0.147–0.419); Si (6.36–604); Sn (0.011–0.630); Sr (4.5–17.80); V (0.401–1.59); Zn (72.93–126.0). The reference intervals obtained in our study can be used to assess the elemental status of the Kalmyk cows bred in the Republic of Sakha (Yakutia) of Russia. The research was carried out in accordance with the state contract No. 19–28 “Implementation of adaptive technologies for keeping specialized beef cattle in the conditions of the Republic of Sakha (Yakutia).”
PSXVI-15 Study of the content of maternal lymphocytes with Barr’s bodies in the bloodstream and red bone marrow of newborn male mice
Authors: Ignateva Anastasia A , Vasileva Svetlana V , Vasilev Roman M , Dushenina Olga , Panova Nataliya
Published: October 2021

In animals with hemochorical placenta, the transfer of maternal antibodies and white blood cells to the fetus can take place along the transplacentary pathway. In the first hours of life, the intestines of newborn animals are able to absorb antibodies from colossus by pinocytosis. Similarly, maternal lymphocytes can get into the blood of a newborn. The aim of this work was to identify maternal lymphoid cells with sexual x-chromatin in the blood and red bone marrow of newborn male mice. Studies were conducted on nonlinean lactating mice and newborn male mice. Two groups of five animals were formed. The mice had breast glands. A longitudinal incision was made and a print smear was prepared. The bone marrow was taken from the proximal part of the femur. Blood was obtained by amputating the tip of the tail. All the drugs were stained by Pappenheim and examined with immersion optics. In the study of breast tissue smears, the number of lymphocytes in the microscope fields was counted. Visualization of drugs showed their high frequency of occurrence (15,1±1.7 in 100 fields of vision). In three-day male mice, the percentage of lymphocytes in the blood was 55.4±3.5%, including cells with x-chromatin (2.4±0.2±%). of which 5.3±3.2% were maternal cells with Barra Taurus. The appearance of maternal lymphocytes in the systemic circulation of newborn male mice is explained by the possibility of their transfer through the hemochorial placenta or absorption into colos dairy through the intestines. Active migration of maternal lymphocytes from the systemic circulation of young animals to the red bone marrow is necessary for the formation of cellular immunity in the body during the period of growth and development.
PSXVI-14 Study of the antagonistic properties of C. utilis in relation to the resident microflora of the animal organism
Authors: Kolodina Evgenia , Nikanova Daria A
Published: October 2021

C. utilis yeast is widely used in industry for the production of fodder protein, amino acids, vitamins, and enzymes. We have studied the antimicrobial activity of C. utilis CY 302 against such collection strains as E. coli 3912/41, S. flexneri 1a 8516, S. enterica serovar Typhimurium 79, S. epidermidis ATCC 14990, S. aureus ATCC 25923, Proteus vulgaris HX 19 222, L. casei subsp. Rhamnosus ATCC 7469, B. breve ATCC 15701, C. albicans ATCC 10231 by using the agar blocks method and by measuring the inhibition growth zone (IGZ, mm) accompanied by the target cells death zone. The yeast strain was grown on Sabouraud’s medium at 28±1 °C for 3 days, and the test cultures were grown on differential diagnostic media for each bacterial species (HiMedia Laboratories Pvt. Ltd, India) at 35±2 °C overnight. The analyses were done separately for each culture. A weak antimicrobial effect was observed with respect to L. casei and B. breve, the IGZ was 5.0 and 4.0 mm, respectively. The halos of inhibition against pathogenic bacteria were 7 mm for E. coli, 5 mm for S. typhimurium, 5 mm for P. vulgaris, 6 mm for S. fiexneri, 6 mm for S. aureus, and 5 mm for S. epidermidis. After incubation there was noted the absence of DZZR in the wild C. utilis CY 302 strain in relation to the C. albicans ATCC 10231 strain from the collection. As a result of the studies the antagonistic potential of the yeast isolate C. utilis CY 302 was revealed. The work was supported by the Ministry of Science and Higher Education of Russia.
PSV-B-27 The effects of carbohydrate hydrocolloids on firmness, toughness, and expressible moisture of wet pet food
Authors: Dainton Amanda N , Dogan Hulya , Aldrich Charles G
Published: October 2021

In a previous experiment, inclusion of guar gum, kappa carrageenan, locust bean gum, and xanthan gum were observed to influence the texture of wet pet food. However, limited published research has addressed their texture and water holding. Therefore, the objective was to quantify the differences in texture and expressible moisture (EM) due to common hydrocolloids in wet pet foods. Treatments included 1% dextrose (D) and 0.5% guar gum with either 0.5% dextrose (DG), 0.5% kappa carrageenan (KCG), 0.5% locust bean gum (LBG), or 0.5% xanthan gum (XGG) in a chicken-based product processed in three replicate batches. Processing occurred in a still retort at 121 °C and 144.79 kPa for an average 81.25 minute cook cycle and 79.58 minute cooling cycle. Texture analysis was conducted with a modified back extrusion while products remained in the can (n = 5) and firmness (peak force measurement) and toughness (positive area under the curve) were recorded during a 2 cm penetration. Expressible moisture was analyzed by centrifugal methods. Data were analyzed as a 1-way ANOVA with the fixed effect of treatment and the random effect of batch. Fisher’s LSD post hoc test was used to separate means. The KCG treatment exhibited the highest (P < 0.05) firmness (27.00 N) and D and DG the lowest (average 8.75 N) with LBG and XGG intermediate (average 15.59 N). More differentiation was noted in toughness, with D (67 N x mm) less tough (P < 0.05) than DG (117 N x mm). The D treatment had the highest (P < 0.05) EM (49.91%) and was the only treatment to separate into 2 phases. The LBG and XGG treatments had the lowest (P < 0.05) EM (average 16.54%) with DG and KCG intermediate (average 25.26%). Carbohydrate hydrocolloids utilize different mechanisms to provide structure, which likely caused the differences in texture and EM observed.
PSV-B-22 Evaluation of whole soybeans as an ingredient in dog diets: Canine palatability of extruded diets with graded levels of dehulled whole soybeans
Authors: Kim Hee S , Aldrich Charles G
Published: October 2021

Increasing the amount of liquid fat in the ration during extrusion can negatively affect product density and product expansion. High-fat ingredients, such as whole soybeans (WSB), may increase energy density while avoiding production issues. In a preliminary extrusion project, the addition of graded levels of WSB on food processing was evaluated. The objective of this study was to determine the effect of incremental addition of whole soybeans in dry dog food on palatability. Experimental diets were extruded with 0%, 10%, 20%, and 30% WSB (WSB0, WSB10, WSB20, and WSB30, respectively). Palatability was determined with a 2-bowl test by beagle dogs (n = 20) for 2 days with each WSB diet compared to the WSB0. First choice preference and total food consumption were recorded for each dog. Individual intake ratios (IR) were calculated (intake of each diet/total intake). First choice (FC) was analyzed by a Chi2 probability, and the consumption of each diet was compared by a Wilcoxon signed rank test and a 2-way analysis of variance (2-way ANOVA). Dogs had greater (P < 0.05) first choice for WSB diets relative to the WSB0, but there was no significant difference among treatments for the food consumption and intake ratio. When comparing WSB0 to WSB10, FC occurred in 13/40 occasions (P < 0.05). The FC for WSB0 occurred in 12/40 occasions compared to WSB20 and in 11/40 occasions compared to WSB30 (P < 0.05). Overall, dogs favored WSB diets over the control diet showing a higher score in the indication of aroma, but this did not result in higher consumption. In conclusion, palatability was not affected by the inclusion of WSB in the dry dog food.
PSV-B-21 Evaluation of whole soybeans as an ingredient in dog diets: Apparent total tract digestibility and stool quality of extruded diets with graded levels of dehulled whole soybeans by dogs
Authors: Kim Hee S , Aldrich Charles G
Published: October 2021

Increasing the amount of liquid fat to the ration during extrusion can negatively affect product density and product expansion. Ingredients, like whole soybeans (WSB), which are high in fat may aid increased energy density while avoiding production issues. In a preliminary extrusion project, the effects on the process of foods were evaluated, followed by animal evaluation. The objective of this study was to determine the effect of dehulled WSB on nutrient digestibility and stool quality by dogs. Experimental diets were extruded with 0%, 10%, 20%, and 30% WSB (WSB0, WSB10, WSB20, and WSB30, respectively). Seven castrated male and three spayed female Beagles of similar age (6.25 ± 0.452 years) were individually housed and fed the experimental diets. The study was designed as replicated 4 × 4 Latin square where dogs were allowed 9-d adaptation followed by 5-d total fecal collection for each period. Titanium dioxide was added to all diets (0.4%) to serve as an indigestible dietary marker to determine apparent total tract digestibility (ATTD). Least-square means of fecal parameters and ATTD were analyzed with a single degree of freedom contrasts and significance at α = 0.05. There was no difference among treatments for food intake or fecal scores. However, there were linear increases (P < 0.05) in DM fecal output, as is fecal output, fecal moisture, and defecation frequency stools/day as WSB increased in the diets. Apparent total tract digestibility of dry matter, crude protein, acid hydrolyzed ether extract, and gross energy decreased linearly as dogs were fed increasing levels of WSB in the diets (P < 0.05). In conclusion, additional thermal processing before extrusion may improve nutrient digestibility, although stool quality was not affected by the inclusion of WSB.
PSV-B-18 Characterization of the fecal microbiome of healthy adult beagle dogs supplemented with graded levels of bacillus coagulans GBI-30, 6086
Authors: Acuff Heather L , Gaire Tara N , Doerksen Tyler , Lu Andrea , Hays Michael P , Palinski Rachel M , Hardwidge Philip R , Aldrich Charles G
Published: October 2021

This study aimed to evaluate the effect of Bacillus coagulans GBI-30, 6086 on the fecal microbiome of healthy adult dogs. Extruded diets containing graded levels of probiotic applied either to the base ration before extrusion or as a topical coating post-extrusion were randomly assigned to ten individually-housed Beagle dogs (7 castrated males, 3 spayed females) of similar age (5.75 ± 0.23 yr) and body weight (12.3 ± 1.5 kg) in a 5 x 5 replicated Latin square with 16-d adaptation and 5-d total fecal collection for each period. Five dietary treatments were formulated to deliver a dose of 0-, 6-, 7-, 8-, or 9-log10 CFU·dog-1·d-1. Fresh fecal samples (n=50) were analyzed by 16S rRNA gene pyrosequencing. Community diversity was evaluated in R (v4.0.3, R Core Team, 2019). Relative abundance data were analyzed using a mixed model (v9.4, SAS Institute, Inc., Cary, NC) with treatment and period as fixed effects and dog as a random effect. Results were considered significant at P < 0.05. Predominant phyla were Firmicutes (mean 81.2% ± 5), Actinobacteria (mean 9.9% ± 4.4), Bacteroidetes (mean 4.5% ± 1.7), Proteobacteria (mean 1.3% ± 0.7), and Fusobacteria (mean 1.1% ± 0.6). No evidence of shifts in predominant phyla, class, family, or genus taxonomic levels were observed except for the Bacillus genus, which had a greater relative abundance (P = 0.0189) in the low probiotic coating and high probiotic coating treatment groups compared to the extruded probiotic group. Alpha-diversity indices (Richness, Chao1, ACE, Shannon, Simpson, Inverse Simpson, and Fisher) and beta-diversity metrics (principal coordinate analysis and multi-dimensional scaling) were similar for all treatments. This data indicates that supplementation with Bacillus coagulans GBI-30, 6086 at a dose of up to 9 log10 CFU·d-1 did not alter the overall diversity of the fecal microbiome of healthy adult dogs over a 21-d period.
PSIII-18 Identification of microbial interactions and markers associated with Shiga toxin-producing bacteria colonization in the rectum of beef steers
Authors: Pan Zhe , Chen Yanhong , McAllister Tim A
Published: October 2021

This study aimed to identify whether microbial interactions in the rectum contribute to Shiga toxin producing bacteria colonization. In total, 12 rectal digesta samples based on the previously identified Shiga toxin 2 gene (stx2) abundance (DNA) and expression (RNA) in Shiga toxin-producing bacteria (Stx2- group: detectable DNA, n=6; Stx2+ group: detectable DNA and RNA, n = 6) were subjected to microbial profiling using amplicon sequencing. Firmicutes (72.7 ± 2.0 %) and Bacteroidetes (24.6 ± 1.9 %) are the most predominant phyla of rectal microbiota, and no compositional differences were identified between two groups at the phylum level. The Shannon and Chao1 indices weren’t different in rectal digesta microbial communities between two groups. Twenty-four and thirteen taxa were identified to be group-specific genera in microbial communities from Stx2- and Stx2+ group, respectively (2 out of 6, average relative abundance >0.1%). The network analysis indicated 12 and 14 keystone taxa (Generalists, densely connected with other taxa) in microbial communities between Stx2- and Stx2+ groups, respectively. Eight out of 12 and six out of 14 generalists in the Stx2- and Stx2+ group are belonging to group-specific genera, respectively. Generalists belonging to group-specific genera were broadly distributed in Stx2- network while centralized distributed in the Stx2+ network, suggesting the higher stability of the Stx2- network structure in comparison of Stx2+ network computed by the natural connectivity measurement. However, 66 core genera shared by microbial communities between two groups were not classified into network generalists. Overall, our results indicate microbial crosstalks and keystone taxa in microbial communities between two groups differed, suggesting that the microbial interactions rather than the shifts in taxa abundance may be more important affecting host. Moreover, group-specific genera play a vital ecological role in the microbial interactions, indicating the potential for being microbial markers to differentiate Shiga toxin-producing bacteria colonization in beef cattle.
PSIII-20 Antimicrobial resistance phenotype and genotype of generic Escherichia coli from encapsulated cinnamaldehyde and citral fed-broiler chicken
Authors: Yang Chongwu , Rehman Muhammad Attiq , Yin Xianhua , Carrillo Catherine , Wang Qi , Yang Chengbo , Gong Joshua , Diarra Moussa
Published: October 2021

This study investigated effects of in-feed encapsulated cinnamaldehyde (CIN) and citral (CIT) alone or in combination (CIN+CIT) on antimicrobial resistance (AMR) phenotypes and genotypes of E. coli isolated from feces of 6-, 16-, 23- and 27-days old broiler chickens. Five dietary treatments included the basal diet (NC), the basal diet supplemented with 55 ppm bacitracin (BAC), 100 ppm encapsulated CIN, CIT, or CIN+CIT. Antimicrobial susceptibility using a Sensititre method of 240 E. coli isolates showed that the most frequent resistances were against b-lactam, aminoglycoside, sulfonamide and tetracycline, however, the prevalence of AMR decreased (P < 0.05) when birds aged. The prevalence of resistance to amoxicillin-clavulanic acid, ceftiofur, ceftriaxone, cefoxitin, gentamicin, and sulfonamide was lower (P < 0.05) in isolates from CIN or CIN+CIT compared to those from NC or BAC. The whole-genome sequencing analysis of 227 of the 240 isolates detected 26 AMR genes (ARGs) and 19 plasmids but the prevalence of some ARGs and plasmid numbers were lower (P < 0.05) in E. coli isolated from CIN or CIN+CIT than NC or BAC. The most prevalent resistance genes included tet(A) (n = 108), aac3_Vla (n = 91), aadA1 (n = 86), blaCMY-2 (n = 78), sul1 (n = 77), aph3_lb (n = 58), aph6_ld (n = 58), and sul2 (n = 24). Interestingly, the number of most virulence genes (VGs) increased (P < 0.05) over time from 6 to 27 days of age. The prevalence of isolates of serotype O21:H16 was lower (P < 0.05) in CIN and CIN+CIT while colibacillosis-associated multi-locus sequence typing (ST117) was the most prevalent in isolates from day 23. A whole genome-based phylogenetic tree revealed a close relationship of 25 of 227 isolates to human or broiler extraintestinal pathogenic E. coli. This study indicates that AMR and virulence genotypes of E. coli could be modulated by encapsulated CIN or CIN+CIT feed supplementations and prompt further investigations on the involved mechanisms.
PSIII-21 Efficacy of deoxynivalenol detoxification by sodium metabisulfite (SMBS) containing nanofibrous mats using an in vitro intestinal epithelial cell IPEC-J2
Authors: Yu Changning , Litke Quintin , Lu Peng , Liu Shangxi , Gong Joshua , Liu Song , Yang Chengbo
Published: October 2021

Deoxynivalenol (DON) occurs in many commonly used cereal grains. Pigs feed with DON concentrations as low as 0.6–2.0 mg/kg can result in reduced feed intake and growth rate, damage to intestinal epithelial cells, and increase susceptibility to enteric pathogens. Sodium metabisulfite (SMBS) can efficiently detoxify DON by converting it 8-DONS or 10-DONS in vitro. However, if SMBS is added directly to the feed, SMBS rapidly degrades under acidic aqueous conditions (e.g. pig stomach) and little SMBS is delivered to the intestinal absorption site where it can effectively detoxify DON. Thus, the objective of this study was to encapsulate SMBS into Eudragit L100-55 nanofibrous mats to deliver intact SMBS to the small intestine and evaluate the efficacy of DON detoxification in the simulated intestine fluid (SIF) using an in vitro intestinal epithelial cell (IPEC-J2) model. Nanofibrous mats were produced by coaxial electrospinning, with peak loading capacity and loading efficiency of SMBS reaching 32.00% and 80.01%. DON-induced cytotoxicity was not observed during in vitro analysis consisting of incubation of DON in the presence of SMBS-containing nanofibers (0.5% w/w) in simulated gastric fluid (SGF) for 2 h followed by incubation in a mixture of SGF and SIF (1:1) for 20 min. Meanwhile, compared to the DON treatment, incubation of DON in the presence of SMBS-containing nanofiber (0.5% w/w) in SGF for 2 h and SIF for 20 min decreased the gene expression of inflammatory cytokines in the IPEC-J2 cells and maintained the cell integrity. To conclude, SMBS released from Eudragit L100-55 nanofibrous mats in the SIF effectively decreased the adverse effects induced by DON in the IPEC-J2 cells. Nanofibrous mats can release a large amount of SMBS in a short time in SIF to achieve the effect of detoxifying DON.
PSXIII-27 Oklahoma State University constructing diversified learning for extension educators
Authors: Markey Jazmin A , Riggs Angel , Moehlenpah Alexi , Lalman David , Stein Dan
Published: October 2021

The role of extension educators is to convey cutting-edge information to the varying sectors of the agricultural industry. In recent years, Oklahoma State University has initiated the process of creating online continuing education courses for Oklahoma-based extension educators. The objective is to develop online modules to enhance educator professional development through expansion of animal science knowledge. In the spring of 2021, Oklahoma extension educators will receive the opportunity to enroll in the introductory level course of this series, “Livestock Production Practices 101” (LPP 101). LPP 101 has been designed for extension educators that have little to no animal science background. The course will be comprised of eight lessons. The eight lessons include livestock species terminology, vision, hearing and handling, nutrient classification, digestive anatomy, reproductive anatomy, life cycles - from conception to consumption, breeds and genetic evaluation parameters, and comparative anatomy. Each lesson will convey species-specific information relative to cattle, horses, pigs, sheep, goats, and chickens. Lessons will include activities such as readings and interactive illustrations. At the conclusion of each lesson, knowledge checks will be given as review to prepare educators for a quiz. Knowledge checks and quiz questions will be a combination of fill-in the blank, multiple choice, matching, and species-specific sorting. Educators must obtain an eighty percent or higher on all lesson quizzes to receive a course certificate. Educators will complete approximately five hours of course work over a ten-week period. A survey will be offered at the conclusion of the course to assess whether the educator found the course to be both engaging and beneficial. The survey will also serve as a guide as to which topics provided in this course would be sought after if developed into courses of their own.
PSXI-22 Study of biosorption mechanisms of movable forms of lead by soil isolates of the genus Bacillus in experiments in vitro
Authors: Terekhova Nadezhda , Galaktionova Lyudmila , Sizentsov Alexey , Davydova Olga , Nikiyan Hike
Published: October 2021

The research was supported by the Ministry of Science and Higher Education in accordance with the state assignment for Ural State Mining University No. 0833-2020-0008 ‘Development and environmental and economic substantiation of the technology for reclamation of land disturbed by the mining and metallurgical complex based on reclamation materials and fertilizers of a new type’. We obtain the scientific results by using funds of the Center for the collective use of scientific equipment of the Federal Scientific Center of biological systems and agricultural technologies of RAS as well (No Ross RU.0001.21 PF59, the Unified Russian Register of Centers for Collective Use - http://www.ckp-rf.ru/ckp/77384).The search for natural bioremediators to restore crop and grazing lands in conditions of the increased anthropogenic load is a relevant and promisingly significant area of research. Thus, we were tasked to assess the prospect of using the physiological and adaptive characteristics of microorganisms of the genus Bacillus as an active biosorbent of movable forms of lead from the substrate on the in vitro model.Soil isolates of microorganisms of the genus Bacillus extracted from samples from areas with increased lead levels were used to achieve our aim. Pb(NO3)2 solutions in the concentration range from 1 to 0.063M were used as the factor regulating the growth. Methods used in the work were: the method of agar basins, the nephelometric method, atomic-absorption spectroscopy, and atomic force microscopy. B. subtilis and B. cereus were isolated from soil samples. The maximum resistance to different concentrations of Pb(NO3)2 was revealed for B. subtilis. The presence of lead cations in the nutrient substrate significantly increased the population density to 26.0%. At the same time, the percentage of lead sorption in the initial stage of the stationary growth phase was 65.3% with the maximum localization on the surface structures of the cell membrane.
PSXI-18 Nitrogen fertilization management of winter pastures for stocker cattle production
Authors: Scaglia Guillermo
Published: October 2021

Bermudagrass is often over-seeded with a cool-season annual grass for grazing purposes. In the Gulf Coast region, summer perennials will not become dormant until there are low soil and ambient temperatures. Nitrogen fertilizer is an expensive input and its split application for winter pastures is recommended. The objective of this experiment was to evaluate the time of application of N fertilizer or its substitution with red clover as a source of N. Three treatments were evaluated in 3 consecutive years (2 replicates per year) on annual ryegrass (cv. Marshall) no-tilled planted (45 kg/ha) on Tifton-85 bermudagrass: 1) Urea in late November and mid-late January; 2) Red clover planted with annual ryegrass; 3) Urea in January only. Thirty crossbred steers (203±24 kg) were blocked by BW and randomly assigned to 1 of 6 groups. Each group were continuously stocked on 1.34 ha pastures for 90 d. Forage mass, botanical composition, and nutritive value of the pastures were determined from the time of the first N application and every 15 d thereafter. There was treatment x year interaction (P = 0.03) in botanical composition. Greater proportions (P = 0.03) of bermudagrass were present on the 2-application treatment on every sampling date until d 15 of the grazing period in Year 1 and 3. This was a result of N absorbed by bermudagrass which impacted its nutritive value (P = 0.01) although not forage production (P = 0.07). In Year 2, annual ryegrass dominated the swards due to a cold artic front in early November. Steers grazing fertilized treatments had greater gains (P = 0.04), while production per ha was greater (P = 0.03) when N was applied in January than when no N was applied. Hay production (kg DM/ha) was similar (P = 0.06) among treatments. Opportunities for improved economics of production and enhanced sustainability appear to be realistic objectives with changes in fertilization strategy.
PSXI-11 Assessing in situ rumen degradability of late season kudzu (Pueraria montana var. lobata)
Authors: Adkins J B , Gulizia J P , Downs Kevin M , Cui S
Published: October 2021

Kudzu (Pueraria montana var. lobata) is an invasive weed species native to eastern Asia affecting much of the southeastern United States. Its broad leaves and viny growth allow it to outcompete native plant species for sunlight and nutrients. Kudzu, however, is a leguminous plant, making it a potential feedstock for ruminant species. Browsing ruminants in areas affected by kudzu could benefit animal productivity while serving to ameliorate rapid plant growth. This study specifically sought to assess the overall rumen degradability, rate of digestion, digestible fraction, and indigestible fraction using an in situ methodology. In situ rumen degradability was analyzed using four ruminally fistulated steers as individual experimental units over two repeated trials. Samples were incubated at 1, 12, 24, 36, 48, 60, and 72 h. Kudzu used in these trials was collected during September, making analysis in this study a reflection of degradability toward the end of the growing season. Data were analyzed as a randomized complete block design with repeated measures showing no significant differences between steers or trials (P > 0.05). Overall degradability across all steers and times was 69.79%. Significant changes in dry matter disappearance across all steers were observed at 1, 12, and 24 h (P < 0.05) with values of 33.86, 64.78, and 74.26%, respectively, and highest observed degradability at 72 h (79.55%). Incubation times between 24 and 72 h were not used in determining rate of digestion as dry matter disappearances throughout these times were not significantly different (P > 0.05). Rate of digestion (kd) was determined, using linear regression, to be 1.68% ∙ h-1 along with a digestible fraction (Do) of 28.29% and indigestible fraction (U) of 22.03%. The results of this study reflect that kudzu maintains a relatively high level of rumen degradability toward seasonal senescence, making it a functional feedstock even into the cooler months.
PSII-B-29 Effects of piglet cross-fostering on pre-weaning growth
Authors: Young Jennifer M , Byrd Christopher J
Published: October 2021

The objective of this study was to evaluate the effect of cross-fostering on piglet growth during lactation. Entire litters (n=40) were allocated to 1 of 2 treatments: control (CON) and foster. Three piglets (FOS) from each CON litter were randomly selected and moved to a foster litter 12 to 24 h post-farrowing, where they were nursed along resident (RES) piglets until weaning (approximately 18 d of age). Piglets were weighed at birth and every 3 d until 15 d of age. Average daily gain (ADG) was calculated through 15 d of age. Data were analyzed using the MIXED procedure in SAS. There was a treatment by day interaction (P = 0.003). At birth, RES piglets were heavier than CON piglets (P = 0.02), while FOS piglets were not significantly different from CON or RES piglets (P = 0.07 and 0.52, respectively). However, CON piglets were numerically heavier than RES piglets on day 15 (P = 0.07), with FOS piglets being intermediate to but not significantly different from CON and RES piglets (P = 0.38 and 0.61, respectively). While weights between CON and RES piglets were significantly different at birth and tended to be different on day 15, there was no difference in ADG from birth to day 15 (P = 0.69). Overall, the results from this study showed no significant detrimental effects of cross-fostering on pre-weaning growth.
PSVIII-24 The effect of growth rate on cytokine presence in uterine environment in beef heifers
Authors: Chaney Elizabeth M , Schneider Liesel G , Payton Rebecca R , McLean Kyle J
Published: October 2021

Heifer development is the foundation of performance in breeding herds. Growth rate during development may alter uterine immune responses impacting reproductive efficiency and, thus, productivity of that animal. Angus-cross heifers (n = 48) were blocked by BW [4 blocks; weight class (WC)], placed in pens (4 heifers per pen), and pens (n = 12) were randomly assigned treatments (4 pens per treatment): 1) control; targeted 0.68 kg/day, 2) high; targeted 1.25 kg/day, and 3) low; targeted 0.11 kg/day. A randomized complete block design with pen as the experimental unit utilized mixed model ANOVAs to determine if treatment influenced BW, BCS, and uterine cytokines. Beginning at the start of treatment (d 0) BW and BCS were taken every 2 weeks. Uterine flushes were performed at d 0 and d 57. Cytokine concentrations above the detectable limit were categorized into a quartiles (1=0–25%; 2=25–50%; 3=50–75%; 4=75–100%). Samples that were below the detection limit were assigned a category value of 0. At the end of the study high treatment pens had greater BW compared to control and low pens (P = 0.01). Concentrations of MCP-1, TNFα, IL-17A, IL-8, and VEGF-A decreased from d 0 to d 57 (P < 0.05). Pens on the high treatment tended to have IL-17A concentrations in the upper quartiles compared to control and low (P = 0.06). Pens in WC 1 and 2 tended to be in the lower quartile score for VEGF-A compared with WC 4 (P = 0.07) and WC 3 was intermediate. In conclusion, these data show that growth rate and size during heifer development may impact cytokine profiles in the uterine environment of nulliparous heifers. This may impact the ability to conceive early in the first breeding season, thus potentially altering long-term reproductive efficiency.
PSXIII-5 Effects of B vitamins and hydroxy trace minerals supplementation on hepatic metabolism of beef cattle at finishing phase in pasture
Authors: Lopes Mariana , Brito Thaís , Lage Josiane F , Costa Thaís , Fontes Marta , Serão Nick V , Mendes Tiago , Reis Ricardo , Duarte Marcio
Published: October 2021

Vitamin B and trace minerals are crucial molecular signals involved in many biological pathways of energy metabolism and their bioavailability is compromised in high-producing ruminant animals. Despite the current knowledge of the effects of vitamin B complex and trace minerals on animal performance, their use as a rumen-protected form and its impact on liver metabolism in finishing beef cattle is poorly known. The present study aimed to assess the effects of ruminally protected B-vitamin blend (Trouw Nutrition, Italy) and hydroxy trace minerals (Cu and Zn; Micronutrients Inc., Indianapolis, IN) on hepatic proteome. A total of 20 non-castrated Nellore males with 353 ± 43 kg of initial BW were submitted in a high supplementation (1.7% of BW) in pasture and randomly assigned to one of the following treatments: CTRL – inorganic trace minerals without supplementation of vitamin B blend; SUP – supplementation of hydroxy trace minerals (Cu and Zn) and vitamin B blend (B5, B6, B7, B9, and B12). All animals were fed the same level of the experimental diet for 106 days and liver biopsy was performed at the end of the experimental period. We use shotgun proteomics combined with biological and network analyses of the protein differentially abundant between treatments, showing 37 proteins differentially abundant (P < 0.10) between treatment groups, where all proteins were up-regulated in the SUP treatment. These proteins were related to protein folding (P = 0.0456), oxidoreductase complex (P = 0.0155), mitochondrial respiratory chain complex IV (P = 0.0132), chaperonin-containing T-complex 2 (P = 0.0132), glutathione metabolism (P = 0.00026) and other linked to oxidative stress regulation. These results indicate that ruminal-protected vitamin B and hydroxy trace minerals supplementation during the finishing phase cause positive changes in the abundance of proteins related to oxidative metabolism and response to oxidative damage.
PSXIII-11 Evaluating different doses of rumen-protected or nonprotected tributyrin on performance of feedlot lambs fed a moderate or low-forage diet
Authors: Watanabe Daniel H , Górka Pawel , Santin Elizabeth , Leclerc Helene , Penner Gregory B
Published: October 2021

The objective of this study was to evaluate performance of lambs when supplemented with different doses and forms of tributyrin in moderate (MF) and low forage (LF) diets. Eighty-four ram lambs were blocked by initial BW (27.9 ± 2.3 kg) assigned into 1 of 2 groups that were fed a MF (40% forage) or LF (10% forage) diet (%DM). Lambs in each block were also assigned to 1 of 6 treatments (n = 7) including a control (no supplementation) or diets that contained 0.1, 0.2, or 0.3% (DM basis) rumen protected (RPT) or 0.1 or 0.3% of tributyrin that was not protected (NPT). Lambs were housed indoor in individual pens and fed once daily for 97 d. At harvest, hot carcass weight (HCW) and back-fat thickness was measured for each lamb. Within diet type (MF and LF), data were analyzed to determine the linear and quadratic response for increasing RPT, the effect of using tributyrin (con vs. RPT and NPT), and the effect of rumen protection (RPT vs. NPT). Treatments fed MF diets did not differ with each other (P ≥ 0.06) for final BW (53.5 kg), ADG (255 g/d), DMI (1.42 kg/d), gain:feed ratio (0.187 kg/kg), backfat thickness (14.1mm), hot carcass weight (24.0 kg), and dressing percentage (45.0%). Also, no differences (P ≥ 0.08) were detected for final BW (56.0 kg) and ADG (0.30 kg/d), DMI (1.56 kg/d), gain:feed ratio (0.190 kg/kg), back-fat thickness (16.8 mm), and HCW (25.5 kg) between treatments fed LF diets. However, lambs fed LF diet with RPT had greater (P = 0.04) dressing percentage than lambs fed NPT (45.9 vs. 44.5%, respectively). We conclude there is no benefit from inclusion of tributyrin in MF diets for lambs whether provided in protected or unprotected form. However, feeding RPT in LF diets for lambs may enhance dressing percentage.
PSXIV-10 Ruminal microbiome of cattle changes with the feeding of fat-containing fish supplements
Authors: Shajahmetova Elvina , Ryazanov Vitalii S , Shejjda Elena , Lebedev Svyatoslav , Miroshnikov Sergej , Duskaev Galimzhan
Published: October 2021

There is insufficient information on the effect of different sources of fat in the diet on the activity and composition of the ruminal microbiome of cattle. The aim of the study was to assess the effect of secondary raw materials - fish oil (1 ml per 100 ml of ruminal fluid; FO) on the microbiome rum of cattle against the background of control (c). The experiment was conducted using the ruminal liquid of the Bulls of the dairy breed (age 12 months, The average live weight of 240–245 kg, diet: hay-70%; grain feed-30%). As a model in vitro used incubator ANKOM Daisy II (USA). Microflora was analyzed with the help of high-performance 16S sequencing of RRNA genes (Illumina). (R≤0.05), which included classes of clostridium (28.6%), bacilli (15.1%), Negativicutes (10.5%), two large families Ruminococcaceae (7.6%) and Lachnospiraceae (16.5%) Bacteroids (39.2%), which included Bacteroidia (38.0%), Preellaceae (25.0%), Prevottell (22.4%). (R≤0.05), Verrucomicrobia (4.5%) (R≤0.05). Clostridia bacteria decreased by 13.8% and Negativicutes by 7.2% compared to C. Fo use showed an increase in bacterial content for bacteroids (57.7%) (R≤0.05) and selected families: Prevotellaceae (32.6%), unclassified Bacteroidales (19.02%), Porrophymonadacee (4.7%) and childbirth: Prevotella (28.8%), Paraprevotella (2.4%). and The Class 5 unit (4.5%) for the FO group showed significant growth. This study was carried out with the financial support of the 0761-2019-0005 project.
PSXIV-12 Sources of variation in the nutritive value of hemp seed meal
Authors: Jacobson Kristen J , Kinman Lea A , Smith William B , Muir James P
Published: October 2021

The 2018 Farm Bill, with its groundbreaking hemp legislation, offered an alternative protein source to livestock producers. Hemp is the agricultural product of Cannabis sativa L. which must contain less than 0.3% tetrahydrocannabinol, the psychoactive component common to marijuana. Hemp oil, cold-pressed from C. sativa seed, has been gaining popularity over recent years for its use in cosmetics, pharmaceuticals, and human food products. Like most oilseeds, this leaves an oilseed meal with a potential for inclusion in livestock feeds. Thus, our objective was to quantify the sources of variation in the nutritive value of hempseed meal. Hempseed meal samples were obtained from commercial oil processing facilities (n = 4). Across these facilities, samples represented 15 independent hempseed meal batches. Our experimental design was a completely randomized design including the random effects of source, batch within source, and replicate within batch by source (laboratory replication). Samples were assayed for NDF, ADF, ADL, CP, and IVTD. Source had no contribution to variance for NDF, ADF, ADL, or CP (P ≥ 0.20). However, batch within source had a significant contribution to variation for NDF (μ = 50.1%; P = 0.01), ADF (μ = 36.8%; P = 0.01), ADL (μ = 12.9%; P < 0.01), and CP (μ = 30.9%; P < 0.01). Irrespective of the differences in nutritive value, there was no contribution to variation (P ≥ 0.23) of any measured effect on IVTD (μ = 53.0%). Results are interpreted to mean that, while variation exists among hempseed meal samples with respect to nutritive value, the viability of hempseed meal as a livestock feed source is strong.
PSXVI-26 Using synchrotron/globar techniques to reveal synergistic and interactive association between molecular structures and nutrient supply in enzymatic and thermal treated oat tissue and whole grain
Authors: Lang C , Yu P
Published: October 2021

As an advanced technique, synchrotron radiation-based microspectroscopy SR-FTIRM has been a rapid, direct, non-destructive and non-invasive bioanalytical method. Globar molecular spectroscopic technique -attenuated total reflectance-ATR-FTIR spectroscopy will be used. For this research, feed type CDC Nasser, forage type CDC haymaker, and milling types CDC Arborg and Summit with three consecutive years are studied. There are three treatments for oat samples: Treatment 1, steam pressure processing alone; Treatment 2, adding innovative fibrolytic enzyme; Treatment 3, steam pressure processing plus fibrolytic enzyme. Each treatment combination has three replications. The objectives of this research are to detect the molecular structure spectral features of processed oat endosperm tissues at a molecular and cellular level in relation to chemical profiles, protein and carbohydrate fractions, energy profiles, degradation kinetics, intestinal digestibility, microbial protein production and true nutrient supply of whole oat grains. This research reveals the interactive association between induced molecular structure changes and nutrient properties and true nutrient supply. This research is also to increase economic returns to oat producers and related dairy industries through efficient utilization of feed-type or milling type of oat grains.
PSXI-26 Blend of essential oils supplemented alone or combined with exogenous amylase compared with virginiamycin on performance of finishing lambs
Authors: Arteaga-Wences Yesica , Estrada-Angulo Alfredo , Franco Manuel , Vasconcellos Guilherme S , Acedo Tiago S , de Carvalho Victor Valério , Perdigão Alexandre , Castro-Pérez Beatriz , Ríos-Rincón Francisco , Urías-Estrada Jesús , Barreras Alberto , Plascencia Alejandro
Published: October 2021

Forty-eight Pelibuey × Katahdin (27.87±4.71 kg) intact lambs were used in a growth-performance trial lasted 87 days to assess the effects of treatments. Lambs were assigned to one of six weight groupings in 24 pens (6 replicas per treatment). Basal diet was comprised by cracked corn (14% CP and 2.08 Mcal ENm/kg) and was supplemented as follows: 1) No additives (Control); 2) supplemented with a blend of essential oils at dose of 150 mg EO/kg diet DM (EO, CRINA® Ruminants, DSM Nutritional Products); 3) supplemented with 150 mg EO plus 560 mg alpha-amylase/kg diet DM (EO+ENZ, RONOZYME® Rumistar, DSM Nutritional Products), and 4) supplemented with antibiotic virginiamycin (VM, Stafac 500, Phibro Animal Health) at dose of 28 mg virginiamycin/kg diet DM. Growth performance and dietary energetics were very similar (P > 0.97) between EO and VM. In such way that, compared to Control, lambs supplemented with EO and VM tended (P = 0.09) to shown greater final weight (3.1%) and average daily gain (ADG; 5.9%) without difference on DMI (P = 0.99); thus, gain-to-feed ratio (GF) and observed dietary net energy (NE) were average improved in 5.7% (P < 0.01). Compared to Controls, combining EO plus ENZ tended to increased DMI (6.8%, P = 0.09), but increased in 10.4% ADG (P < 0.01) and in 4.4% (P = 0.02) feed efficiency. Lambs supplemented with EO shown lower (P ≤ 0.10) kidney-pelvic-heart fat than Controls and VM lambs. Compared to Controls, additive supplementation decreased (P ≤ 0.03) the relative weight of intestines, the effect being more pronounced with EO supplementation. Compared to VM, supplemental EO (alone or combined with enzyme) shown lower (10.1%, P ≤ 0.04) visceral fat. Supplemental essential oils is a viable alternative to virginiamycin in finishing high-energy diets for lambs. Combining essential oils plus amylase can be a strategy to promote better daily gain weights allowing target final weight at shorter time.
PSXI-29 Effects of feed restriction on blood constituent concentrations in hair sheep
Authors: Tadesse Dereje L , Puchala Ryszard , Gipson Terry A , Goetsch Arthur L
Published: October 2021

Forty-six Dorper, 47 Katahdin, and 41 St. Croix female sheep (initial body weight of 62, 62, and 51 kg, respectively, SEM=1.43; 3.8±0.18 yr of age) from 45 commercial farms in Midwest, Northwest, Southeast, and central Texas regions of the United States were used to evaluate effects of feed restriction on concentrations of blood constituents. A 50% concentrate pelleted diet was fed, with the amount varied in the first 4 wk to achieve stable BW. The amount of feed offered in wk 5–10 was set at 55% of that consumed in wk 3–4. Blood was sampled at the end of wk 3, 4, 6, 8, and 10, with constituent levels in wk 4 and 10 assumed relevant to conditions with different maintenance energy requirements (i.e., fed at maintenance and approximately 43% lower with restricted intake). There were some differences among breeds such as ones based on samples collected at all times in urea nitrogen (14.0, 13.7, and 15.4 mg/dl; SEM=0.31) and creatinine (0.945, 0.836, and 0.809 mg/dl for Dorper, Katahdin, and St. Croix, respectively; SEM=0.0253) but relatively few among regions and only one interaction between week and breed or region. There was a trend for a difference (P = 0.051) between wk 4 and 10 in the concentration of glucose (51.9 and 54.2 mg/dl; SEM=0.90), and there were differences (P < 0.05) in levels of lactate (23.9 and 20.3 mg/dl; SEM=0.89), urea N (16.4 and 13.0 mg/dl; SEM= 0.25), creatinine (0.808 and 0.919 mg/dl; SEM=0.0165), triglycerides (31.8 and 25.5 mg/dl; SEM=0.63), cholesterol (67.5 and 74.7 mg/dl; SEM=1.66), and cortisol (10.55 and 8.31 ng/ml for wk 4 and 10, respectively; SEM=0.0542). In conclusion, similar responses of different hair sheep breeds in blood constituent levels to feed restriction is in accordance with comparable effects on body weight and the maintenance energy requirement previously reported.