bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic Sleep Deprivation Altered the Expression of Memory-Related Genes and Caused Cognitive Memory Dysfunction in Mice
Authors: Wang Xiang , Chen Hanqing , Tang Tian , Zhan Xiang , Qin Shu , Hang Taijun , Song Min
Published: October 2024

Lack of sleep, whether acute or chronic, is quite common and negatively affects an individual’s memory and cognitive function. The question of whether chronic sleep deprivation (CSD) causes cognitive impairment to arise and progress is not well studied. To investigate the effects of CSD on memory and cognition, this study began by establishing a CSD mouse model. Behavioral experiments on animals revealed that CSD induced cognitive behavioral abnormalities reminiscent of Alzheimer’s disease. Western blot experiments further demonstrated a considerable increase in amyloid-β (Aβ) expression in the mouse brain following CSD. Meanwhile, the hub gene Prkcg was searched for in the cerebellum using RNA-seq and bioinformatics analysis. PKCγ (Prkcg) expression was significantly reduced, as demonstrated by RT-qPCR and Western blot validations. Additionally, CSD was associated with downregulated CREB expression, decreased expression of the endothelin-converting enzyme (ECE1), and increased phosphorylation of ERK1/2 downstream of PKCγ. These findings suggested that CSD down-regulated PKCγ expression, decreased ECE1 expression, impaired Aβ degradation, and affected the PKCγ/ERK/CREB pathway and the synthesis of memory-related proteins. Overall, this study highlighted how CSD modulated PKCγ-related metabolism, impacting Aβ clearance and the production of memory-related proteins. Such insights are crucial for understanding and preventing sporadic Alzheimer’s disease (sAD) associated with CSD.
Metabolomics-Based Machine Learning for Predicting Mortality: Unveiling Multisystem Impacts on Health
Authors: Oravilahti Anniina , Vangipurapu Jagadish , Laakso Markku , Fernandes Silva Lilian
Published: October 2024

Reliable predictors of long-term all-cause mortality are needed for middle-aged and older populations. Previous metabolomics mortality studies have limitations: a low number of participants and metabolites measured, measurements mainly using nuclear magnetic spectroscopy, and the use only of conventional statistical methods. To overcome these challenges, we applied liquid chromatography–tandem mass spectrometry and measured >1000 metabolites in the METSIM study including 10,197 men. We applied the machine learning approach together with conventional statistical methods to identify metabolites associated with all-cause mortality. The three independent machine learning methods (logistic regression, XGBoost, and Welch’s t-test) identified 32 metabolites having the most impactful associations with all-cause mortality (25 increasing and 7 decreasing the risk). From these metabolites, 20 were novel and encompassed various metabolic pathways, impacting the cardiovascular, renal, respiratory, endocrine, and central nervous systems. In the Cox regression analyses (hazard ratios and their 95% confidence intervals), clinical and laboratory risk factors increased the risk of all-cause mortality by 1.76 (1.60–1.94), the 25 metabolites by 1.89 (1.68–2.12), and clinical and laboratory risk factors combined with the 25 metabolites by 2.00 (1.81–2.22). In our study, the main causes of death were cancers (28%) and cardiovascular diseases (25%). We did not identify any metabolites associated with cancer but found 13 metabolites associated with an increased risk of cardiovascular diseases. Our study reports several novel metabolites associated with an increased risk of mortality and shows that these 25 metabolites improved the prediction of all-cause mortality beyond and above clinical and laboratory measurements.
The Importance of Phosphoinositide 3-Kinase in Neuroinflammation
Authors: Wright Brock , King Samuel , Suphioglu Cenk
Published: October 2024

Neuroinflammation, characterised by the activation of immune cells in the central nervous system (CNS), plays a dual role in both protecting against and contributing to the progression of neurodegenerative diseases, such as Alzheimer’s disease (AD) and multiple sclerosis (MS). This review explores the role of phosphoinositide 3-kinase (PI3K), a key enzyme involved in cellular survival, proliferation, and inflammatory responses, within the context of neuroinflammation. Two PI3K isoforms of interest, PI3Kγ and PI3Kδ, are specific to the regulation of CNS cells, such as microglia, astrocytes, neurons, and oligodendrocytes, influencing pathways, such as Akt, mTOR, and NF-κB, that control cytokine production, immune cell activation, and neuroprotection. The dysregulation of PI3K signalling is implicated in chronic neuroinflammation, contributing to the exacerbation of neurodegenerative diseases. Preclinical studies show promise in targeting neuronal disorders using PI3K inhibitors, such as AS605240 (PI3Kγ) and idelalisib (PI3Kδ), which have reduced inflammation, microglial activation, and neuronal death in in vivo models of AD. However, the clinical translation of these inhibitors faces challenges, including blood–brain barrier (BBB) permeability, isoform specificity, and long-term safety concerns. This review highlights the therapeutic potential of PI3K modulation in neuroinflammatory diseases, identifying key gaps in the current research, particularly in the need for brain-penetrating and isoform-specific inhibitors. These findings underscore the importance of future research to develop targeted therapies that can effectively modulate PI3K activity and provide neuroprotection in chronic neurodegenerative disorders.
Integrating Microarray Data and Single-Cell RNA-Seq Reveals Key Gene Involved in Spermatogonia Stem Cell Aging
Authors: Hashemi Karoii Danial , Azizi Hossein , Skutella Thomas
Published: October 2024

The in vitro generation of spermatogonial stem cells (SSCs) from embryonic stem cells (ESCs) offers a viable approach for addressing male infertility. A multitude of molecules participate in this intricate process, which requires additional elucidation. Despite the decline in SSCs in aged testes, SSCs are deemed immortal since they can multiply for three years with repeated transplantation. Nonetheless, the examination of aging is challenging due to the limited quantity and absence of precise indicators. Using a microarray, we assessed genome-wide transcripts (about 55,000 transcripts) of fibroblasts and SSCs. The WGCNA approach was then used to look for SSC-specific transcription factors (TFs) and hub SSC-specific genes based on ATAC-seq, DNase-seq, RNA-seq, and microarray data from the GEO databases as well as gene expression data (RNA-seq and microarray data). The microarray analysis of three human cases with different SSCs revealed that 6 genes were upregulated, and the expression of 23 genes was downregulated compared to the normal case in relation to aging genes. To reach these results, online assessments of Enrich Shiny GO, STRING, and Cytoscape were used to forecast the molecular and functional connections of proteins before identifying the master routes. The biological process and molecular function keywords of cell–matrix adhesion, telomerase activity, and telomere cap complex were shown to be significantly altered in upregulated differentially expressed genes (DEGs) by the functional enrichment analysis. According to our preliminary research, cell-specific TFs and TF-mediated GRNs are involved in the creation of SSCs. In order to maximize the induction efficiency of ESC differentiation into SSCs in vitro, hub SSC-specific genes and important SSC-specific TFs were identified, and sophisticated network regulation was proposed. According to our research, these genes and the hub proteins that they interact with may be able to shine a light on the pathophysiologies of infertility and aberrant germ cells.
Single-Cell RNA Sequencing Reveals Monocyte-Derived Interstitial Macrophages with a Pro-Fibrotic Phenotype in Bleomycin-Induced Pulmonary Fibrosis
Authors: Wang Shunli , Li Jie , Wu Caixia , Lei Zhengyao , Wang Tong , Huang Xinxin , Zhang Suxia , Liu Yuting , Bi Xiaohan , Zheng Fanshuo , Zhu Xuyou , Huang Ziling , Yi Xianghua
Published: October 2024

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive lung disease with limited effective therapies. Interstitial macrophages (IMs), especially those derived from monocytes, play an unknown role in IPF pathogenesis. By using single-cell RNA sequencing (scRNA-seq), bleomycin (BLM)-induced pulmonary fibrosis mouse lungs were analyzed to characterize the cellular landscape and heterogeneity of macrophages in this model. scRNA-seq was used to identify distinct interstitial macrophage subpopulations in fibrotic lungs, with monocyte-derived macrophages exhibiting a pro-fibrotic gene expression profile enriched in wound healing, extracellular matrix (ECM) remodeling, and pro-fibrotic cytokine production functions. A pseudotime analysis revealed that IMs originated from monocytes and differentiated along a specific trajectory. A cell–cell communication analysis demonstrated strong interactions between monocyte-derived interstitial macrophages (Mo-IMs) and fibroblasts through the transforming growth factor beta (TGFβ), secreted phosphoprotein 1 (SPP1), and platelet-derived growth factor (PDGF) signaling pathways. Flow cytometry validated the presence and expansion of Mo-IMs subpopulations in BLM-treated mice. This study reveals the cellular heterogeneity and developmental trajectory of lung macrophages in early BLM-induced pulmonary fibrosis, highlighting the crucial role of Mo-IMs with a pro-fibrotic phenotype in IPF pathogenesis via interactions with fibroblasts. Targeting these specific macrophage subpopulations and associated signaling pathways may provide novel therapeutic strategies for IPF.
Mechanisms Underlying Sensory Nerve-Predominant Damage by Methylmercury in the Peripheral Nervous System
Authors: Nakano Tsuyoshi , Yoshida Eiko , Sasaki Yu , Kazama Shigekatsu , Katami Fumika , Aoki Kazuhiro , Fujie Tomoya , Du Ke , Hara Takato , Yamamoto Chika , Takahashi Tsutomu , Fujiwara Yasuyuki , Eto Komyo , Iwakura Yoichiro , Shinoda Yo , Kaji Toshiyuki
Published: October 2024

Sensory disturbances and central nervous system symptoms are important in patients with Minamata disease. In the peripheral nervous system of these patients, motor nerves are not strongly injured, whereas sensory nerves are predominantly affected. In this study, we investigated the mechanisms underlying the sensory-predominant impairment of the peripheral nervous system caused by methylmercury. We found that the types of cell death in rat dorsal root ganglion (DRG) neurons caused by methylmercury included apoptosis, necrosis, and necroptosis. Methylmercury induced apoptosis in cultured rat DRG neurons but not in anterior horn neurons or Schwann cells. Additionally, methylmercury activated both caspase 8 and caspase 3 in DRG neurons. It increased the expression of tumor necrosis factor (TNF) receptor-1 and the phosphorylation of receptor-interacting protein kinase 3 (RIP3) and mixed-lineage kinase domain-like pseudokinase (MLKL). The expression of TNF-α was increased in macrophage-like RAW264.7 cells by methylmercury. The increase was suggested to be mediated by the NF-κB pathway. Moreover, methylmercury induced neurological symptoms, evaluated by a hindlimb extension response, were significantly less severe in TNF-α knockout mice. Based on these results and our previous studies, we propose the following hypothesis regarding the pathogenesis of sensory nerve-predominant damage by methylmercury: First, methylmercury accumulates within sensory nerve neurons and initiates cell death mechanisms, such as apoptosis, on a small scale. Second, cell death triggers the infiltration of macrophages into the sensory fibers. Third, the macrophages are stimulated by methylmercury and secrete TNF-α through the NF-κB pathway. Fourth, TNF-α induces cell death mechanisms, including necrosis, apoptosis through the caspase 8/3 pathway, and necroptosis through the TNFR1-RIP1-RIP3-MLKL pathway, activated by methylmercury in sensory neurons. Consequently, methylmercury exhibits potent cytotoxicity specific to the DRG/sensory nerve cells in the peripheral nervous system. This chain of events caused by methylmercury may contribute to sensory disturbances in patients with Minamata disease.
Structural, Antioxidant, and Protein/DNA-Binding Properties of Sulfate-Coordinated Ni(II) Complex with Pyridoxal-Semicarbazone (PLSC) Ligand
Authors: Jevtovic Violeta , Golubović Luka , Alshammari Odeh A. O. , Alhar Munirah Sulaiman , Alanazi Tahani Y. A. , Radulović Aleksandra , Nakarada Đura , Dimitrić Marković Jasmina , Rakić Aleksandra , Dimić Dušan
Published: October 2024

The pyridoxal-semicarbazone (PLSC) ligand and its transition metal complexes have shown significant biological activity. In this contribution, a novel nickel(II)-PLSC complex, [Ni(PLSC)(SO4)(H2O)2], was obtained, and its structure was determined by X-ray crystallographic analysis, FTIR, and UV-VIS spectroscopy. The sulfate ion is directly coordinated to the central metal ion. The intermolecular stabilization interactions were examined using Hirshfeld surface analysis. The crystal structure was optimized by a B3LYP functional using two pseudopotentials for nickel(II) (LanL2DZ and def2-TZVP) together with a 6-311++G(d,p) basis set for non-metallic atoms. The experimental and theoretical bond lengths and angles were compared, and the appropriate level of theory was determined. The stabilization interactions within the coordination sphere were investigated by the Quantum Theory of Atoms in Molecules (QTAIM). The antioxidant activity towards hydroxyl and ascorbyl radicals was measured by EPR spectroscopy. The interactions between Human Serum Albumin (HSA) and the complex were examined by spectrofluorimetric titration and a molecular docking study. The mechanism of binding to DNA was analyzed by complex fluorescence quenching, potassium iodide quenching, and ethidium bromide displacement studies in conjunction with molecular docking simulations.
Core/Shell ZnO/TiO2, SiO2/TiO2, Al2O3/TiO2, and Al1.9Co0.1O3/TiO2 Nanoparticles for the Photodecomposition of Brilliant Blue E-4BA
Authors: Dolatyari Mahboubeh , Tahmasebi Mehdi , Dolatyari Sudabeh , Rostami Ali , Zarghami Armin , Yadav Ashish , Klein Axel
Published: October 2024

The synthesis and characterization of ZnO/TiO2, SiO2/TiO2, Al2O3/TiO2, and Al1.9Co0.1O3/TiO2 core/shell nanoparticles (NPs) is reported. The NPs were used for photocatalytic degradation of brilliant blue E-4BA under UV and visible light irradiation, monitored by high-performance liquid chromatography and UV-vis absorption spectroscopy. The size of the NPs ranged from 10 to 30 nm for the core and an additional 3 nm for the TiO2 shell. Al2O3/TiO2 and Al1.9Co0.1O3/TiO2 showed superior degradation under UV and visible light compared to ZnO/TiO2 and SiO2/TiO2 with complete photodecomposition of 20 ppm dye in 20 min using a 10 mg/100 mL photocatalyst. The “Co-doped” Al1.9Co0.1O3/TiO2 NPs show the best performance under visible light irradiation, which is due to increased absorption in the visible range. DFT-calculated band structure calculations confirm the generation of additional electronic levels in the band gap of γ-Al2O3 through Co3+ ions. This indicates that Co-doping enhances the generation of electron–hole pairs after visible light irradiation.
Comparative Transcriptome Analysis of the Pest Galeruca daurica (Coleoptera: Chrysomelidae) Larvae in Response to Six Main Metabolites from Allium mongolicum (Liliaceae)
Authors: Li Ling , Li Jinwei , Wang Haichao , Li Yanyan , Dong Ruiwen , Pang Baoping
Published: October 2024

Plants are important ecological factors and food resources, which can significantly affect the occurrence and distribution of insects. The metabolites in host plants can affect the feeding, spawning, and avoidance behaviors of herbivorous insects. Galeruca daurica (Joannis) is a phytophagous pest that has seriously occurred in the desert steppe of Inner Mongolia in recent years, only infesting the leaves of Allium plants. In order to clarify the effects of plant metabolites on the gene expression in G. daurica larvae at the transcriptome level, we fed the larvae of G. daurica with Allium tuberosum leaves soaked in 10% DMSO solutions containing d-galactose, β-d-glucopyranose, l-rhamnose, isoquercitrin, isoflavone, and rutin, respectively, used the larvae fed on A. tuberosum leaves soaked in a 10% DMSO solution as the control, and screened out the differentially expressed genes (DEGs) by performing high-throughput transcriptome sequencing. The results showed that a total of 291 DEGs were identified compared to the solvent control (DMSO), including 130, 34, 29, 21, 72, and 97 in the isoquercitrin, isoflavone, rutin, d-galactose, β-d-glucopyranose, and l-rhamnose treatment groups, respectively. GO and KEGG enrichment analysis showed that most DEGs were enriched in various metabolic pathways, implying that these six main primary and secondary metabolites in Allium plants may affect various metabolic processes in the larvae of G. daurica.
Stridulatory Organs and Sound Recognition of Three Species of Longhorn Beetles (Coleoptera: Cerambycidae)
Authors: Wei Jia-Quan , Wang Xiao-Yun , Zheng Xia-Lin , Tong Xin
Published: October 2024

Sound is an important medium of communication among insects. Some longhorn beetles produce sounds during their daily activities, and these sounds play a role in courtship, predation, and defense. However, whether there are differences in the sounds emitted by longhorn beetles and how to distinguish and recognize these sounds have not been investigated in detail. Here, the sounds of Glenea cantor (Fabricius), Moechotypa diphysis (Pascoe), and Psacothea hilaris (Pascoe) were collected, and the differences in their stridulatory organs were observed and compared using scanning electron microscopy (SEM). The characteristics of their sounds were analyzed using MATLAB. Linear prediction cepstral coefficients (LPCC) and Mel frequency cepstral coefficients (MFCC) were used to extract the sound features, and the support vector machine (SVM) model was used to identify the sounds of three species. The results showed that the stridulatory organs of three species of longhorn beetles differed in morphology and time domain, and the combination of MFCC and SVM had a better recognition ability. The difference in the stridulatory organs of longhorn beetles may be an important reason for the differences in the sounds they produce, and we discussed the application of insect sounds in insect classification.
Results on the Use of an Original Burner for Reducing the Three-Way Catalyst Light-Off Time
Authors: Clenci Adrian , Cioc Bogdan , Berquez Julien , Iorga-Simăn Victor , Stoica Robert , Niculescu Rodica
Published: October 2024

Individual road mobility comes with two major challenges: greenhouse gas emissions related to global warming and chemical pollution. For the pollution reduction in the spark ignition engine vehicle, the standard and reliable aftertreatment technology is the three-way catalytic converter (TWC). However, the TWC starts to convert once an optimal temperature, usually known as the light-off temperature, is reached. There are many methods to reduce the warm-up period of the TWC, among which is using a burner. The initial question underlying this study was to see if the use of a relatively straightforward extra-combustion device mounted upstream the TWC, without complex elements, was able to serve the purpose of reducing the light-off time. Consequently, an original burner was designed and investigated numerically via the CFD method and experimentally via measurements of the temperature evolution within a TWC, along with the emissions specific to the burner’s operation. The main findings of this study are: (1) the CFD-based examination is a good way to decide on how to achieve the so-called fit-for-purpose internal aerodynamics of the burner (i.e., to obtain a homogeneous mixture) and (2) to reach the light-off temperature, conventionally taken as 500 K, the burner was operated for 5.2 s, i.e., 3.6 g of gasoline injected, 2.7 g of CO2 and 1.351 g of CO, respectively, emitted. Moreover, this study identified measures for improving the burner’s design as well as an enhanced procedure for the burner’s operating control both aiming to produce a cleaner combustion during the TWC pre-heating.
A Proof-of-Concept Open-Source Platform for Neural Signal Modulation and Its Applications in IoT and Cyber-Physical Systems
Authors: Ghani Arfan
Published: October 2024

This paper presents the design, implementation, and characterization of a digital IoT platform capable of generating brain rhythm frequencies using synchronous digital logic. Designed with the Google SkyWater 130 nm open-source process design kit (PDK), this platform emulates Alpha, Beta, and Gamma rhythms. As a proof of concept and the first of its kind, this device showcases its potential applications in both industrial and academic settings. The platform was integrated with an IoT device to optimize and accelerate research and development efforts in embedded systems. Its cost-effective and efficient performance opens opportunities for real-time neural signal processing and integrated healthcare. The presented digital platform serves as a valuable educational tool, enabling researchers to engage in hands-on learning and experimentation with IoT technologies and system-level hardware–software integration at the device level. By utilizing open-source tools, this research demonstrates a cost-effective approach, fostering innovation and bridging the gap between theoretical knowledge and practical application. Furthermore, the proposed system-level design can be interfaced with various serial devices, Wi-Fi modules, ARM processors, and mobile applications, illustrating its versatility and potential for future integration into broader IoT ecosystems. This approach underscores the value of open-source solutions in driving technological advancements and addressing skills shortages.
The Effectiveness of Emotional Freedom Techniques for Depressive Symptoms: A Meta-Analysis
Authors: Seok Ji-Woo , Kim Jaeuk U.
Published: October 2024

Background: Emotional Freedom Techniques (EFT) have gained attention as a potential therapy for reducing depressive symptoms. However, the evidence remains inconsistent. This meta-analysis aims to assess the overall efficacy of EFT in treating depressive symptoms and explore moderators influencing its effectiveness. Methods: A meta-analysis of 18 randomized controlled trials (RCTs) was conducted, with depressive symptom reduction as the primary outcome. Meta-regression explored moderators such as the EFT format, duration, age, and depression severity. Results: The analysis showed a significant overall effect size of 1.268 for EFT in reducing depressive symptoms. A moderator analysis revealed that group-based EFT interventions were more effective than individual ones, and participants with moderate depression experienced the greatest benefits. Additionally, shorter interventions were found to be highly effective. Conclusions: EFT effectively reduces depressive symptoms, particularly in group settings and for those with moderate depression. Shorter, well-structured interventions may enhance treatment efficiency. Further studies should explore long-term effects and broader applications.
Impact of COVID-19 on Diaphragmatic Function: Understanding Multiorgan Involvement and Long-Term Consequences
Authors: Pietranis Katarzyna Anna , Kostro Amanda Maria , Dzięcioł-Anikiej Zofia , Moskal-Jasińska Diana , Kuryliszyn-Moskal Anna
Published: October 2024

The COVID-19 pandemic has brought significant attention to the respiratory system, with much focus on lung-related disorders. However, the diaphragm, a crucial component of respiratory physiology, has not been adequately studied, especially in the context of long COVID. This review explores the multipotential role of the diaphragm in both respiratory health and disease, emphasizing its involvement in long-term complications following SARS-CoV-2 infection. The diaphragm’s fundamental role in respiratory physiology and its impact on balance and posture control, breathing patterns, and autonomic nervous system regulation are discussed. This review examines complications arising from COVID-19, highlighting the diaphragm’s involvement in neurological, musculoskeletal, and inflammatory responses. Particular attention is given to the neuroinvasive impact of SARS-CoV-2, the inflammatory response, and the direct viral effects on the diaphragm. The diaphragm’s role in long COVID is explored, with a focus on specific symptoms such as voice disorders, pelvic floor dysfunction, and sleep disturbances. Diagnostic challenges, current methods for assessing diaphragmatic dysfunction, and the complexities of differentiating it from other conditions are also explored. This article is the first to comprehensively address diaphragmatic dysfunction resulting from COVID-19 and long COVID across various physiological and pathological aspects, offering a new perspective on its diagnosis and treatment within a multisystem context.
Differences in Physical Demands and Player’s Individual Performance Between Winning and Losing Quarters on U-18 Basketball Players During Competition
Authors: Miró Adrià , Vicens-Bordas Jordi , Beato Marco , Salazar Hugo , Coma Jordi , Pintado Carles , García Franc
Published: October 2024

Background: This study examines how physical demands and individual performance influence quarter results in under-18 basketball players during a six-day basketball tournament. Methods: Twelve male players from an elite Spanish team were tracked using inertial microsensors to monitor external load variables (player load, steps, and dynamic stress load). Individual performance was assessed using the performance index rating (PIR). Results: The results showed significant differences in physical demands between quarters. Also, player load (F = 3.75, p = 0.012) and steps (F = 5.29, p = 0.001) were higher in the first quarter and decreased over time. Winning quarters had significantly higher physical demands compared to losing quarters (PL: F = 27.13, p < 0.001; steps: F = 16.70, p < 0.001; DSL: F = 9.50, p < 0.001). On the contrary, PIR did not show significant differences between winning and losing quarters (F = 2.15, p = 0.143), but tended to be higher in winning quarters. Conclusions: These results suggest that physical demands are stronger predictors of quarter results than individual performance scores, indicating that such parameters should be closely monitored by sport scientists and coaches since they can play a crucial role in team success.
Rosmarinic Acid Exhibits Antifungal and Antibiofilm Activities Against Candida albicans: Insights into Gene Expression and Morphological Changes
Authors: Aydin Merve , Unusan Nurhan , Sumlu Esra , Korucu Emine Nedime
Published: October 2024

Candida species, opportunistic pathogens that cause various infections, pose a significant threat due to their ability to form biofilms that resist antifungal treatments and immune responses. The increasing resistance of Candida spp. and the limited availability of effective treatments have prompted the research of natural compounds as alternative therapies. This study assessed the antifungal properties of RA against Candida species, focusing on its impact on C. albicans biofilms and the underlying mechanisms. The antifungal efficacy of RA was evaluated using the CLSI M27-A3 microdilution method on both fluconazole-susceptible and -resistant strains. Biofilm formation by C. albicans was assessed through a crystal violet assay, while its antibiofilm activity was analyzed using an MTT assay and field emission scanning electron microscopy (FESEM). Gene expression related to biofilm formation was studied using quantitative real-time PCR (qRT-PCR), and statistical analysis was performed with an ANOVA. Among the 28 Candida strains tested, RA exhibited minimum inhibitory concentration (MIC) values ranging from 160 to 1280 μg/mL. At a 640 μg/mL concentration, it significantly reduced the expression of genes associated with adhesion (ALS3, HWP1, and ECE1), hyphal development (UME6 and HGC1), and hyphal cAMP-dependent protein kinase regulators (CYR1, RAS1, and EFG1) in RAS1-cAMP-EFG1 pathway (p < 0.05). FESEM analysis revealed a reduction in hyphal networks and disruptions on the cell surface. Our study is the first to demonstrate the effects of RA on C. albicans adhesion, hyphae development, and biofilm formation through gene expression analysis with findings supported by FESEM. This approach distinguishes our study from previous studies on the effect of RA on Candida. However, the high MIC values of RA limit its antifungal potential. Therefore, more extensive research using innovative methods is required to increase the antifungal effect of RA.
Media Events in the Digital Age: Analysis of the Treatment of Elizabeth II and Juan Carlos I During the State Funeral
Authors: Martín-Llaguno Marta , Navarro-Beltrá Marián , Berganza Rosa , García-Almarcha Lucía
Published: October 2024

Digitalisation has redefined both media events and monarchical communication by enabling the diverse and critical participation of journalists and citizens. Media events that were once dominated by official narratives are now subject to multiple real-time transformations, with competing storylines emerging. This study examines the treatment of two monarchical figures (Queen Elizabeth II and King Juan Carlos I) during “the first major state funeral in the digital age” when the official invitation to Juan Carlos I generated a debate about his status and sparked curiosity about a potential photo. From an initial collection of 100,000 tweets and 1520 news articles, 187 pieces simultaneously mentioning both monarchs were selected and analysed to compare their treatment. In contrast to the British portrayal linked to professionalism and tradition, the Spanish media—and especially the social networks—immerse Juan Carlos I in controversy. A planned event in which strategic institutional messages were launched serves as an excuse for criticism and polarisation around the monarchy. This confirms that digitalisation has not only altered the way people access and participate but has also redefined the narratives of even the most traditional events. These transformations pose significant challenges to the image management of institutions such as the monarchy.
Non-Surgical Approaches to the Management of the Intrathoracic Goiter—A Systematic Review
Authors: Miani Cesare , Locatello Luca Giovanni , Caiazza Nicole , Bergamin-Bracale Anna Maria , Rigo Stefania , Rugiu Maria Gabriella , Zuin Andrea , Simo Ricard
Published: October 2024

Background: Intrathoracic goiters (ITGs) are usually managed by surgical excision, However, over recent years, non-surgical strategies are emerging as an alternatives for treating this condition. Methods: A systematic review of research published since 2017 in the PubMed database was conducted and a total of 39 articles were retrieved, along with methodological issues and future directions in the research on ITGs. Results: Several non-surgical treatments exist, including radio-iodine ablation (RIA) and mini-invasive approaches, such as transcervical microwave ablation (TcMA), transcervical radiofrequency ablation (TcRfA), or selective embolization of the thyroid arteries (SETA). Despite encouraging reports, their current use remains limited. Conclusions: Treatment of ITGs requires a multidisciplinary thyroid team, and when non-surgical options are chosen, patients need to be carefully selected, and their outcomes must be prudently considered and discussed with the patient.
Aquariums as Research Platforms: Characterizing Fish Sounds in Controlled Settings with Preliminary Insights from the Blackbar Soldierfish Myripristis jacobus
Authors: Almunia Javier , Fernández-Maquieira María , Flores Melvin
Published: October 2024

This study highlights the potential of aquariums as research platforms for bioacoustic research. Aquariums provide access to a wide variety of fish species, offering unique opportunities to characterize their acoustic features in controlled settings. In particular, we present a preliminary description of the acoustic characteristics of Myripristis jacobus, a soniferous species in the Holocentridae family, within a controlled environment at a zoological facility in the Canary Islands, Spain. Using two HydroMoth 1.0 hydrophones, we recorded vocalizations of the blackbar soldierfish in a glass tank, revealing a pulsed sound type with a peak frequency around 355 Hz (DS 64), offering a more precise characterization than previously available. The vocalizations exhibit two distinct patterns: short sequences with long pulse intervals and fast pulse trains with short inter-pulse intervals. Despite some limitations, this experimental setup highlights the efficacy of cost-effective methodologies in public aquariums for initial bioacoustic research. These findings contribute to the early stages of acoustic characterization of coastal fishes in the western central Atlantic, emphasizing the value of passive acoustic monitoring for ecological assessments and conservation efforts. Moreover, this study opens new avenues for considering the acoustic environment as a crucial factor in the welfare of captive fish, an aspect that has largely been overlooked in aquarium management.
Evaluating Policy Shifts on Perceived Greenspace Quality: Applying Regression Discontinuity During the COVID-19 Reopening Period
Authors: Lin Chensong , Jia Chenjie , Wang Baisen , Kang Shuhao , Chen Hongyu , Li Di , Wu Longfeng
Published: October 2024

Abstract: Urban greenspaces have been essential in supporting residents’ well-being during the COVID-19 pandemic, particularly under strict lockdown measures. However, the impact of changing containment policies on residents’ perceived greenspace quality remains insufficiently explored. This study utilized online survey data collected between 11 October and 29 December 2022, in Shanghai, coinciding with the major policy shift on 5 December 2022. A probability proportionate to size sampling was adopted to survey residents aged 18 and above who had lived in the city for at least six months, yielding a total of 577 valid responses. We assessed residents’ perceived greenspace quality using 20 park- and community-level variables, focusing on both overall quality and specific features of greenspaces. A regression discontinuity design (RDD) was applied to evaluate how the lifting of the COVID-19 policies influenced residents’ perceptions of parks and community greenspaces. Our RDD estimation indicates no statistically significant change in residents’ overall perceived quality of parks after the policy shift, except for increased satisfaction with specific features such as plant diversity, maintenance, seating areas, trails, and large open spaces. In contrast, residents who responded after the policy shift reported a significantly higher perceived quality of community greenspaces compared to those who completed the survey before the shift (0.609 score difference, p < 0.01), with notable increases in satisfaction regarding plant diversity, maintenance, and seating areas. Perception of plant quantity remained unchanged in both types of greenspaces. Residents expressed greater satisfaction with sports facilities in parks, while community greenspaces were preferred for their water features and esthetic qualities. By adopting an RDD with a unique dataset, this study contributes empirical evidence to the current ongoing debate on the role of urban greenspace during the later stages of COVID-19. Specifically, it examines how changes in public health policy and the resulting increase in mobility might affect residents’ perceived greenspace quality. The findings can assist decision-makers and urban planners in developing more adaptive strategies to address the diverse needs of residents for greenspaces during the transitional period of a public health crisis.
Resilience of Interurban Public Transport and Impact of COVID-19 on Rural Connectivity in Sparsely Populated Regions
Authors: Vega Naranjo Juan Miguel , González-Escobar Rafael , Jiménez-Espada Montaña , Vivas Jonathan Galeano
Published: October 2024

The aim of this research consisted of assessing the effects of the COVID-19 pandemic on the interurban public transport system in a rural region with a sparse population density, considering the number of tickets sold and passengers in each locality, as well as the different connecting lines. From a methodological point of view and with the intention of identifying patterns to explain the behaviour of both the routes and passengers, a series of variables were selected, becoming determining factors that sought to offer a solution to the search for a common trend. Additionally, data processing by the means of statistical analysis and the application of Geographic Information Systems (GIS) tools complemented the procedure. The results obtained in the investigation were provided both by municipality and by interurban routes. An interesting finding of this research was the uneven recovery of the municipalities. The localities closest to the attractor nucleus have recovered more quickly to pre-pandemic mobility levels due to their geographical proximity, larger populations, higher incomes per household, and need to access certain public services. In terms of routes, all the lines showed significant decreases in ticket sales, although with variations. Although passenger numbers have shown a gradual recovery, the initial loss was considerable, and pre-pandemic normality has not been completely achieved. This research provides a comprehensive overview of the changes in interurban mobility over a four-year period. The incorporation of critical variables and the segmentation by municipality and route provide a way to identify discernible patterns of mobility. However, the lack of previous research focusing on the impact of the pandemic in rural areas of low population density restricts the possibility of establishing a comparison and to generalise the findings. The authors consider that future research should include other alternative means of transport in these interurban areas and incorporate variables to characterise passengers, such as age, gender, etc.
Reliability and Accuracy of Portable Devices for Measuring Countermovement Jump Height in Physically Active Adults: A Comparison of Force Platforms, Contact Mats, and Video-Based Software
Authors: Bagchi Amritashish , Raizada Shiny , Thapa Rohit K. , Stefanica Valentina , Ceylan Halil İbrahim
Published: October 2024

Measuring countermovement jump (CMJ) height accurately is essential for evaluating lower-body explosive power in athletes and other active populations. With technological advancements, various portable tools have been developed for this purpose, including force platforms, contact mats, and video-based software. This study aimed to (a) investigate the test–retest reliability of the KINVENT K-Deltas force platform for CMJ height measurement and (b) compare its accuracy with a contact mat (Chronojump, Spain) and a video-based software (My Jump app, version 3). Twenty-two physically active collegiate athletes (mean age of 19.7 ± 1.2 years) from various sports backgrounds completed five CMJ trials with simultaneous height measurements using all three tools. Intra-class correlation coefficients (ICC), Cronbach’s alpha, and coefficient of variation (CV) were calculated to assess reliability. In contrast, Pearson correlations and Bland–Altman plots were used to compare device results. The K-Deltas force platform exhibited high test–retest reliability (ICC = 0.981), closely matching the contact mat (ICC = 0.987) and the My Jump app (ICC = 0.986). Correlations between the instruments were strong (force platform vs. contact mat: r = 0.987; force platform vs. My Jump: r = 0.987; contact mat vs. My Jump: r = 0.996), with no between-instrument differences (t-test p = 0.203–0.935, effect size ≤ 0.01–0.16), demonstrating the interchangeability of these tools for practical purposes. However, Bland–Altman analysis revealed limits of agreement between the devices, indicating small but consistent measurement differences. While all instruments were reliable, discrepancies in the absolute values suggest practitioners should consider device-specific variations when comparing CMJ data. These findings highlight the reliability of the K-Deltas force platform as a viable alternative for measuring CMJ height, though differences between devices should be accounted for in applied settings. Therefore, the portable force plates can monitor training, predict injury risk, assess neuromuscular fatigue, and lead to informed decision-making.
Assessing the Impact of Environmental Conditions on Reflectance Values in Inland Waters Using Multispectral UAS Imagery
Authors: Salim Daniel Henrique Carneiro , Andrade Gabriela Rabelo , Assunção Alexandre Flávio , Cosme Pedro Henrique de Menezes , Pereira Gabriel , Amorim Camila C.
Published: October 2024

This study investigates the impact of environmental conditions on reflectance values obtained from multispectral Unmanned Aerial System (UAS) imagery in inland waters, focusing on sun glint, cloud glint, wind-generated waves, and cloud shading projections. Conducted in two reservoirs with differing water qualities, UAS platforms equipped with MicaSense Altum and DJI Phantom 4 Multispectral sensors were used to collect multispectral images. The results show that sun glint significantly increases reflectance variability as solar elevation rises, particularly beyond 54°, compromising data quality. Optimal flight operations should occur within a solar elevation angle range of 25° to 47° to minimize these effects. Cloud shading introduces complex variability, reducing median reflectance. Wind-generated waves enhance sun glint, increasing variability across all spectral bands, while cloud glints amplify reflectance non-uniformly, leading to inconsistent data variability. These findings underscore the need for precise correction techniques and strategic UAS deployment to mitigate environmental interferences. This study offers valuable insights for improving UAS-based monitoring and guiding future research in diverse aquatic environments.
Tribological Properties of Multilayer DLC/MoS2 Nanocomposite Coatings on Microtextured Titanium Alloy Surfaces
Authors: Liu Ke , Ding Qingqing , Peng Hao , Guan Kang , Xi Xiaowan , Kong Ning , Liao Maolin
Published: October 2024

Single surface texture or coating technology is gradually unable to produce lasting lubrication of a TC4 titanium alloy in a harsh environment. In order to address this problem, a rectangular microstructure is prepared on the surface of a TC4 titanium alloy by laser processing, and then MoS2/DLC composite interlayer nanocoatings are prepared on the surface by non-equilibrium magnetron sputtering. Friction and wear tests are then carried out on single fabricated, coated and fabricated coatings. The results show that the MoS2/DLC composite interlayered nanocoating can effectively combine with the texture to achieve better friction reduction compared with the single texture and coating. The textured composite coating has the lowest friction coefficient (reduced from 0.4122 to 0.0978) and wear. Through controlled experiments, the textured coating showed good tribological properties at different temperatures and in different friction cycle tests. This study can effectively improve the tribological properties of metal materials through composite coatings, providing research ideas for enhancing the service life of alloys under long-term friction in high-temperature environments.
Investigating the Mechanical Characteristics and Fracture Morphologies of Basalt Fiber Concrete: Insights from Uniaxial Compression Tests and Meshless Numerical Simulations
Authors: Zhao Chuan , Jiang Guoxin , Guo Junli , Yu Shuyang , Ma Zelong , Zhuang Chunyi , Lei Youbin , Liang Zilin
Published: October 2024

To explore the mechanical properties and fracture modes of basalt fiber-reinforced concrete, single-doped and hybrid-doped basalt fiber-reinforced concrete was prepared, and uniaxial failure tests under different basalt fiber-reinforced concrete contents were carried out. At the same time, the smooth kernel function in the traditional SPH method was improved, and the basalt fiber random generation algorithm was embedded in the SPH program to realize the simulation of the progressive failure of basalt fiber-reinforced concrete. The results show that under the circumstance with no basalt fiber, the specimen final failure mode is damage on the upper and lower surface, as well as the side edge, while the interior of the specimen center is basically intact, indicating that there is an obvious stress concentration phenomenon on the upper and lower surface when the specimen is compressed. Under the circumstance with basalt fiber, longitudinal cracks begin to appear inside the specimen. With the increase in the content, the crack location gradually develops from the edge to the middle, and the crack number gradually increases. This indicates that appropriately increasing the fiber content in concrete may improve the stress state of concrete, change the eccentric compression to axial compression, and indirectly increase the compressive strength of concrete. The numerical simulation results are consistent with the test results, verifying the rationality of the numerical simulation algorithm. For the concrete model without the basalt fiber, shear cracks are generated around the model. For the concrete model with basalt fiber, in addition to shear cracks, the tensile cracks generated at the basalt fiber inside the model eventually lead to the splitting failure of the model. The strength of concrete samples with basalt content of 0.1%, 0.2%, and 0.3% is increased by 1.69%, 5.10%, and 4.31%, respectively, compared to the concrete sample without basalt fiber. It can be seen that with the increase in the content of single-doped basalt fiber, the concrete strength is improved to a certain extent, but the improvement degree is not high; For hybrid-doped basalt fiber-reinforced concrete, the strength of concrete samples with basalt content of 0.1%, 0.2%, and 0.3% is increased by 14.51%, 15.02%, and 30.31%, respectively, compared to the concrete sample without basalt fiber. Therefore, compared with the single-doped basalt fiber process, hybrid doping is easier to improve the strength of concrete.