bluuebunny/crossref_metadata_2025 huggingface dataset:
Life-Course Individual and Neighborhood Socioeconomic Status and Risk of Dementia in the Atherosclerosis Risk in Communities Neurocognitive Study
Authors: George Kristen M , Lutsey Pamela L , Kucharska-Newton Anna , Palta Priya , Heiss Gerardo , Osypuk Theresa , Folsom Aaron R
Published: April 2020

We examined associations of individual- and neighborhood-level life-course (LC) socioeconomic status (SES) with incident dementia in the Atherosclerosis Risk in Communities cohort. Individual- and neighborhood-level SES were assessed at 3 life epochs (childhood, young adulthood, midlife) via questionnaire (2001–2002) and summarized into LC-SES scores. Dementia was ascertained through 2013 using cognitive exams, telephone interviews, and hospital and death certificate codes. Cox regression was used to estimate hazard ratios of dementia by LC-SES scores in race-specific models. The analyses included data from 12,599 participants (25% Black) in the United States, with a mean age of 54 years and median follow-up of 24 years. Each standard-deviation greater individual LC-SES score was associated with a 14% (hazard ratio (HR) = 0.86, 95% confidence interval (CI): 0.81, 0.92) lower risk of dementia in White and 21% (HR = 0.79, 95% CI: 0.71, 0.87) lower risk in Black participants. Education was removed from the individual LC-SES score and adjusted for separately to assess economic factors of LC-SES. A standard-deviation greater individual LC-SES score, without education, was associated with a 10% (HR = 0.90, 95% CI: 0.84, 0.97) lower dementia risk in White and 15% (HR = 0.85, 95% CI: 0.76, 0.96) lower risk in Black participants. Neighborhood LC-SES was not associated with dementia. We found that individual LC-SES is a risk factor for dementia, whereas neighborhood LC-SES was not associated.
A unified approach to the calculation of information operators in semiparametric models
Authors: Mao Lu
Published: April 2020

The infinite-dimensional information operator for the nuisance parameter plays a key role in semiparametric inference, as it is closely related to the regular estimability of the target parameter. Calculation of information operators has traditionally proceeded in a case-by-case manner and has often entailed lengthy derivations with complicated arguments. We develop a unified framework for this task by exploiting commonality in the form of semiparametric likelihoods. The general formula developed allows one to derive information operators with simple calculus and, if necessary at all, a minimal amount of probabilistic evaluation. This streamlined approach shows its simplicity and versatility in application to a number of existing models as well as a new model of practical interest.
The apparently milder course of multiple sclerosis: changes in the diagnostic criteria, therapy and natural history
Authors: Sorensen Per Soelberg , Sellebjerg Finn , Hartung Hans-Peter , Montalban Xavier , Comi Giancarlo , Tintoré Mar
Published: April 2020

In the past decade, changes have occurred in the spectrum of multiple sclerosis courses. The natural history of multiple sclerosis appears milder from the first sign of demyelinating disease to the progressive course, probably as a result of an interplay between several factors including changes in the diagnostic criteria, changes in the epidemiology of multiple sclerosis, impact of early and appropriate disease-modifying treatment and improvement of the general state of health in the population. It has been suggested to regard incidental findings of demyelinating lesions in MRI in individuals without any history of clinical symptoms consistent with neurological dysfunction, so-called radiological isolated syndrome, as the initial course of multiple sclerosis. New diagnostic criteria have enabled the multiple sclerosis diagnosis in many patients at the first clinical demyelinating event, clinically isolated syndrome. The remaining patients with clinically isolated syndrome have a more benign prognosis, and for relapsing-remitting multiple sclerosis, the prognosis has become more favourable. Reduced disease activity in patients with relapsing-remitting multiple sclerosis can partly be ascribed to more efficacious new disease-modifying therapies but decrease in disease activity has also be seen in placebo-treated patients in clinical trials. This may be explained by several factors: change in the diagnostic criteria, more explicit inclusion criteria, exclusion of high-risk patients e.g. patients with co-morbidities, and more rigorous definitions of relapses and disease worsening. However, these factors also make the disease course in patients treated with disease-modifying therapies seem more favourable. In addition, change in the therapeutic target to stable disease (no evidence of disease activity = no relapses, no disease worsening and no MRI activity) could by itself change the course in relapsing-remitting multiple sclerosis. The effectiveness of disease-modifying drugs has reduced the transition from relapsing-remitting to secondary progressive multiple sclerosis. The concept of progressive multiple sclerosis has also evolved from two very distinct categories (primary progressive and secondary progressive multiple sclerosis) to a unified category of progressive multiple sclerosis, which can then be split into the categories of active or inactive. Also, an increasing tendency to treat progressive multiple sclerosis with disease-modifying therapies may have contributed to change the course in progressive multiple sclerosis. In conclusion, during the past decade the entire course of multiple sclerosis from the first sign of a demyelinating disorder through the progressive course appears to be milder due to a complex interplay of several factors.
Thoracoscopic surgery for recurrent tracheoesophageal fistula after esophageal atresia repair
Authors: Hua Kaiyun , Yang Shen , Zhang Yanan , Zhao Yong , Gu Yichao , Li Shuangshuang , Liao Junmin , Huang Jinshi
Published: April 2020

We aimed to investigate the safety, feasibility, and outcomes of thoracoscopic surgery for recurrent tracheoesophageal fistula (rTEF) after esophageal atresia repair. The medical records and follow-up data of 31 patients who underwent thoracoscopic surgery for rTEF at a single institution were collected and reviewed. In total, 31 patients were enrolled with a median age of 7 months (range: 3–30 months) and a median weight of 6,000 g (range: 4,000–12,000 g) before reoperation. The median operation time for the entire series was 2.9 hours (range: 1.5–7.5 hours), and the median total hospitalization duration after surgery was 19 days (range: 11–104 days). One patient died of anastomotic leakage, a second rTEF, severe malnutrition, and thoracic infection; the mortality rate was 3.23% (1/31). Nine patients (9/31, 29.03%) had an uneventful recovery, and the incidences of postoperative anastomotic leakage, anastomotic stricture, and second rTEF were 25.81%, 61.29%, and 9.68%, respectively. After a median follow-up of 12 months (range: 3–24 months), 26 survivors resumed full oral feeding, 2 were tube fed, 2 required a combination of methods, and 4 patients experienced severe respiratory complications. In total, 9 patients had pathological gastroesophageal reflux, and 2 patients eventually underwent Nissen fundoplication. Of the 30 survivors with growth chart data, the median weight for age Z-score, height for age Z-score, and weight for height Z-score were − 0.46 (range: −5.1 to 2.8), 0.75 (range: −2.7 to 4.7), and − 1.14 (range: −6.8 to 3.0), respectively. Thoracoscopic surgical repair for rTEF is safe, feasible, and effective with acceptable mortality and morbidity.
Mincle/Syk Signalling Promotes Intestinal Mucosal Inflammation Through Induction of Macrophage Pyroptosis in Crohn’s Disease
Authors: Gong Wenbin , Zheng Tao , Guo Kun , Fang Miao , Xie Haohao , Li Weijie , Tang Qinqing , Hong Zhiwu , Ren Huajian , Gu Guosheng , Wang Gefei , Wu Xiuwen , Zhao Yun , Ren Jianan
Published: April 2020

Background

Macrophage-inducible C-type lectin [Mincle] signalling plays a proinflammatory role in different organs such as the brain and liver, but its role in intestinal inflammation, including Crohn’s disease [CD], remains unknown.

Methods

The characteristics of Mincle signalling expression in CD patients and experimental colitis were examined. The functional role of Mincle signalling in the intestine was addressed in experimental colitis models in vivo by using Mincle knock-out [Mincle-/-] mice. In addition, neutralising anti-Mincle antibody, downstream spleen tyrosine kinase [Syk] inhibitor, and Mincle pharmacological agonist were used to study the Mincle signalling in intestine. Bone marrow-derived macrophages were collected from mice and used to further verify the effect of Mincle signalling in macrophages.

Results

This study has shown that Mincle signalling was significantly elevated in active human CD and experimental colitis, and macrophages were the principal leukocyte subset that upregulate Mincle signalling. Mincle deficiency and Syk pharmacological inhibition ameliorated the colitis by reducing induced macrophage pyroptosis, and activation of Mincle with the agonist aggravated the intestinal inflammation. The ex vivo studies demonstrated that activation of Mincle signalling promoted the release of proinflammatory cytokines, whereas its absence restricted release of proinflammatory cytokines from pyroptosis of macrophages. In addition, Mincle/Syk signalling in macrophages could promote the production of chemokines to recruit neutrophils by activating mitogen-activated protein kinase [MAPK] during intestinal inflammation.

Conclusions

Mincle signalling promotes intestinal mucosal inflammation by inducing macrophage pyroptosis. Modulation of the Mincle/Syk axis emerges as a potential therapeutic strategy to target inflammation and treat CD.
Long-term β-blocker therapy and clinical outcomes after acute myocardial infarction in patients without heart failure: nationwide cohort study
Authors: Kim Jihoon , Kang Danbee , Park Hyejeong , Kang Minwoong , Park Taek Kyu , Lee Joo Myung , Yang Jeong Hoon , Song Young Bin , Choi Jin-Ho , Choi Seung-Hyuk , Gwon Hyeon-Cheol , Guallar Eliseo , Cho Juhee , Hahn Joo-Yong
Published: April 2020

Aims

To investigate the association between long-term β-blocker therapy and clinical outcomes in patients without heart failure (HF) after acute myocardial infarction (AMI).

Method and results

Between 2010 and 2015, a total of 28 970 patients who underwent coronary revascularization for AMI with β-blocker prescription at hospital discharge and were event-free from death, recurrent myocardial infarction (MI), or HF for 1 year were enrolled from Korean nationwide medical insurance data. The primary outcome was all-cause death. The secondary outcomes were recurrent MI, hospitalization for new HF, and a composite of all-cause death, recurrent MI, or hospitalization for new HF. Outcomes were compared between β-blocker therapy for ≥1 year (N = 22 707) and β-blocker therapy for <1 year (N = 6263) using landmark analysis at 1 year after index MI. Compared with patients receiving β-blocker therapy for <1 year, those receiving β-blocker therapy for ≥1 year had significantly lower risks of all-cause death [adjusted hazard ratio (HR) 0.81; 95% confidence interval (CI) 0.72–0.91] and composite of all-cause death, recurrent MI, or hospitalization for new HF (adjusted HR 0.82; 95% CI 0.75–0.89), but not the risks of recurrent MI or hospitalization for new HF. The lower risk of all-cause death associated with persistent β-blocker therapy was observed beyond 2 years (adjusted HR 0.86; 95% CI 0.75–0.99) but not beyond 3 years (adjusted HR 0.87; 95% CI 0.73–1.03) after MI.

Conclusion

In this nationwide cohort, β-blocker therapy for ≥1 year after MI was associated with reduced all-cause death among patients with AMI without HF.
Orthogonal P-wave morphology, conventional P-wave indices, and the risk of atrial fibrillation in the general population using data from the Finnish Hospital Discharge Register
Authors: Eranti Antti , Carlson Jonas , Kenttä Tuomas , Holmqvist Fredrik , Holkeri Arttu , Haukilahti M Anette , Kerola Tuomas , Aro Aapo L , Rissanen Harri , Noponen Kai , Seppänen Tapio , Knekt Paul , Heliövaara Markku , Huikuri Heikki V , Junttila M Juhani , Platonov Pyotr G
Published: April 2020

Aims

Identifying subjects at high and low risk of atrial fibrillation (AF) is of interest. This study aims to assess the risk of AF associated with electrocardiographic (ECG) markers linked to atrial fibrosis: P-wave prolongation, 3rd-degree interatrial block, P-terminal force in lead V1, and orthogonal P-wave morphology.

Methods and results

P-wave parameters were assessed in a representative Finnish population sample aged ≥30 years (n = 7217, 46.0% male, mean age 51.4 years). Subjects (n = 5489) with a readable ECG including the orthogonal leads, sinus rhythm, and a predefined orthogonal P-wave morphology type [positive in leads X and Y and either negative (Type 1) or ± biphasic (Type 2) in lead Z; Type 3 defined as positive in lead X and ± biphasic in lead Y], were followed 10 years from the baseline examinations (performed 1978–80). Subjects discharged with AF diagnosis after any-cause hospitalization (n = 124) were defined as having developed AF. Third-degree interatrial block was defined as P-wave ≥120 ms and the presence of ≥2 ± biphasic P waves in the inferior leads. Hazard ratios (HRs) and confidence intervals (CIs) were assessed with Cox models. Third-degree interatrial block (n = 103, HR 3.18, 95% CI 1.66–6.13; P = 0.001) and Type 3 morphology (n = 216, HR 3.01, 95% CI 1.66–5.45; P < 0.001) were independently associated with the risk of hospitalization with AF. Subjects with P-wave <110 ms and Type 1 morphology (n = 2074) were at low risk (HR 0.46, 95% CI 0.26–0.83; P = 0.006), compared to the rest of the subjects.

Conclusion

P-wave parameters associate with the risk of hospitalization with AF.
The Impact of Late-Life Disability Spectrum on Depressive Symptoms: A Fixed-Effects Analysis of Panel Data
Authors: Xiang Xiaoling , Yang Yong , Cheng Jianjia , An Ruopeng
Published: April 2020

Objectives

This study examines the impact of a previously validated disability spectrum that accounts for compensatory strategies on depressive symptoms in older adults.

Methods

This study was a secondary data analysis of 2011 through 2018 surveys from the National Health and Aging Trends Study (N = 7,609). The disability spectrum was categorized using a 5-level hierarchical scheme: fully able, successful accommodation, reduced activity, difficulty, and assistance for 12 mobility, self-care, and household activities. The individual fixed-effects panel model was used to examine the impact of this disability spectrum on depressive symptoms.

Results

Depressive symptoms rose progressively with each successive category on the disability spectrum in descriptive analyses. In fixed-effects models, moving from “fully able” to “successful accommodation” was not associated with significant changes in depressive symptoms; this result held for all self-care and mobility activities. Moving from “fully able” to “reduced activity” was associated with a significant increase in depressive symptoms for 3 household activities (doing laundry, making hot meals, and shopping for groceries) but not for paying bills/banking or keeping track of medications. Going up 2 or more stages above “fully able” on the disability spectrum was associated with a significant increase in depressive symptoms across all 12 activities.

Discussion

While limitations in a range of daily activities have harmful effects on mental health, using compensatory strategies that do not erode one’s perception of autonomy can help older adults cope with the psychological detriments of late-life disability.
Reformulation of Parker–Oldenburg's method for Earth's spherical approximation
Authors: Chen Wenjin , Tenzer Robert
Published: April 2020

Parker–Oldenburg's method is perhaps the most commonly used technique to estimate the depth of density interface from gravity data. To account for large density variations reported, for instance, at the Moho interface, between the ocean seawater density and marine sediments, or between sediments and the underlying bedrock, some authors extended this method for variable density models. Parker–Oldenburg's method is suitable for local studies, given that a functional relationship between gravity data and interface geometry is derived for Earth's planar approximation. The application of this method in (large-scale) regional, continental or global studies is, however, practically restricted by errors due to disregarding Earth's sphericity. Parker–Oldenburg's method was, therefore, reformulated also for Earth's spherical approximation, but assuming only a uniform density. The importance of taking into consideration density heterogeneities at the interface becomes even more relevant in the context of (large-scale) regional or global studies. To address this issue, we generalize Parker–Oldenburg's method (defined for a spherical coordinate system) for the depth of heterogeneous density interface. Furthermore, we extend our definitions for gravity gradient data of which use in geoscience applications increased considerably, especially after launching the Gravity field and steady-state Ocean Circulation Explorer (GOCE) gravity-gradiometry satellite mission. For completeness, we also provide expressions for potential. The study provides the most complete review of Parker–Oldenburg's method in planar and spherical cases defined for potential, gravity and gravity gradient, while incorporating either uniform or heterogeneous density model at the interface. To improve a numerical efficiency of gravimetric forward modelling and inversion, described in terms of spherical harmonics of Earth's gravity field and interface geometry, we use the fast Fourier transform technique for spherical harmonic analysis and synthesis. The (newly derived) functional models are tested numerically. Our results over a (large-scale) regional study area confirm that the consideration of a global integration and Earth's sphericty improves results of a gravimetric forward modelling and inversion.
Propagation of EBV-driven Lymphomatous Transformation of Peripheral Blood B Cells by Immunomodulators and Biologics Used in the Treatment of Inflammatory Bowel Disease
Authors: Levhar Nina , Ungar Bella , Kopylov Uri , Fudim Ella , Yavzori Miri , Picard Orit , Amariglio Ninette , Chowers Yehuda , Shemer-Avni Yonat , Mao Ren , Chen Min-hu , Ye Ziyin , Eliakim Rami , Ben-Horin Shomron
Published: April 2020

Background

Immunomodulators and anti tumor-necrosis-α antibodies (anti-TNFs) have been implicated in increased risk of Epstein–Barr virus (EBV)–driven B-cell lymphoproliferative disorders in inflammatory bowel disease (IBD) patients. However, the underlying mechanisms are poorly understood.

Methods

An in-vitro model of lymphoblastoid cell line (LCL) was established by co-incubation of EBV-infected human peripheral blood mononuclear cells (PBMC) with Cyclosporin-A (CSA). After 4 weeks, the resultant LCLs were analyzed by flow cytometry, telomerase activity assay, and next generation sequencing. Subsequently, LCLs were explored in the presence of therapeutic agents for IBD (anti-TNFs, vedolizumab, 6-Mercaptopurine [6MP], methotrexate). Epstein–Barr virus titers were quantitated by real-time polymerase chain reaction.

Results

In cultures of PBMC with EBV and CSA, LCLs were characterized as an expanded, long lived population of CD58+CD23hi B-cells with high telomerase activity and clonal expansion. Upon addition to the cell cultures, LCL percentages were higher with infliximab (median 19.21%, P = 0.011), adalimumab (median 19.85%, P = 0.003), and early washed-out 6MP (median 30.57%, P = 0.043) compared with PBMC with EBV alone (median 9.61%). However, vedolizumab had no such effect (median 8.97%; P = 0.435). Additionally, LCL expansion was accompanied by increase in intracellular, rather than extracellular, EBV viral copies. Compared with PBMC with EBV alone, high levels of LCL were subsequently observed after triple depletion of NK cells, CD4+ T cells, and CD8+ T cells (median 52.8% vs 16.4%; P = 0.046) but also in cultures depleted solely of CD4+ T cells (median 30.7%, P = 0.046).

Conclusions

These results suggest that both anti-TNFs and 6MP, but not vedolizumab, propagate EBV-driven lymphoblastoid transformation in an in vitro model of lymphoma. This model may prove useful for studying mechanisms underlying proneoplastic viral immune interactions of novel drugs in IBD therapy.
Portfolio risk and the quantum majorization of correlation matrices
Authors: Fontanari Andrea , Eliazar Iddo , Cirillo Pasquale , Oosterlee Cornelis W
Published: April 2020

We propose quantum majorization as a way of comparing and ranking correlation matrices, with the aim of assessing portfolio risk in a unified framework. Quantum majorization is a partial order in the space of correlation matrices, which are evaluated through their spectra. We discuss the connections between quantum majorization and an important class of risk functionals, and we define two new risk measures able to capture interesting characteristics of portfolio risk.
Oral cephalosporin and β-lactamase inhibitor combinations for ESBL-producing Enterobacteriaceae urinary tract infections
Authors: Stewart Adam G , Harris Patrick N A , Henderson Andrew , Schembri Mark A , Paterson David L
Published: April 2020

ESBL-producing Enterobacteriaceae as uropathogens have given rise to a sizeable amount of global morbidity. Community and hospital surveillance studies continue to report increasing proportions of these organisms as causes of urinary tract infection (UTI). Due to limited treatment options and the presence of cross-resistance amongst oral antibiotics of different classes, patients often require IV therapy, thereby increasing healthcare costs and reducing the effectiveness of delivering healthcare. Oral cephalosporin antibiotics are well known for their ability to achieve high urinary concentrations, in addition to achieving clinical success for treatment of uncomplicated UTI with a drug-susceptible pathogen. Novel cephalosporin/β-lactamase inhibitor combinations have been developed and demonstrate good in vitro activity against ESBL-producing isolates. A pooled analysis of in vitro activity of existing oral cephalosporin/clavulanate combinations in ESBL-producing Enterobacteriaceae has shown MIC50s of 0.5–1, 0.125–1 and 0.25 mg/L for cefpodoxime, ceftibuten and cefixime, respectively. A novel cyclic boronic acid β-lactamase inhibitor, QPX7728, was able to produce MIC50 values of 0.5 and ≤0.06 mg/L when paired with cefpodoxime and ceftibuten, respectively. Other novel combinations, cefpodoxime/ETX0282 and ceftibuten/VNRX7145, have also demonstrated excellent activity against ESBL producers. Clinical trials are now awaited.
Evaluation of a Genetic Sexing Strain of the Oriental Fruit Fly as a Candidate for Simultaneous Application of Male Annihilation and Sterile Insect Techniques (Diptera: Tephritidae)
Authors: Shelly Todd E
Published: April 2020

The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important pest of fruits and vegetables, and many countries operate surveillance programs to detect infestations and, if needed, implement costly control efforts. The Male Annihilation Technique (MAT), which involves deployment of the male lure methyl eugenol (ME) to reduce or eliminate the male population, has been used as a stand-alone strategy or as a precursor to the Sterile Insect Technique (SIT), which involves the release of sterile males to generate sterile male by wild female crosses and the production of inviable progeny. Modeling suggests that simultaneous implementation of MAT and SIT, rather than sequential deployment, increases the probability of successful eradication. Previous research has shown that B. dorsalis males that have fed on ME show reduced tendency to re-visit ME sources. Also, males fed ME gain a mating advantage over nonfed males. The goal of the present study was to evaluate the suitability of a genetic sexing strain of B. dorsalis as a candidate for concurrent implementation of MAT and SIT. Evaluation focused on the timing of prerelease exposure to methyl eugenol to identify the male age at which feeding upon the lure both i) reduces postrelease attraction to ME-baited traps (thus allowing the operation of MAT) and ii) enhances postrelease mating competitiveness (thus increasing the effectiveness of SIT). Results indicate that prerelease ME feeding by 6-d-old males, with release the following day, would allow effective, concurrent implementation of MAT and SIT.
Color Preference of Three Parasitoids Imported to the Americas for the Biological Control of the Coffee Berry Borer (Curculionidae: Scolytinae)
Authors: Castillo Alfredo , Rojas Julio C
Published: April 2020

The African parasitoids Cephalonomia stephanoderis Waterston (Bethylidae: Hymenoptera), Prorops nasuta Betrem (Bethylidae: Hymenoptera), and Phymastichus coffea LaSalle (Eulophidae: Hymenoptera) are biological control agents of the coffee berry borer (Coleoptera: Curculionidae). In this study, we investigated in laboratory the female behavioral responses of these parasitoids to 14 different wavelengths (340–670 nm) against a control (570 nm, yellow). When nonchooser females were included in the analysis, none parasitoids species showed a preference between 340, 350, 370, 460, 490, 520, 540, 590, 640, and 650 nm with respect to the control wavelength. In contrast, the three species of parasitoids were more attracted to wavelengths of 380, 400, and 420 nm than the control wavelength. Phymastichus coffea and P. nasuta were more attracted to the wavelength of 400 and 420 nm compared to C. stephanoderis. At 380 nm, P. coffea and C. stephanoderis wasps showed the higher responses in comparison to P. nasuta females. When nonchooser wasps were excluded from the analysis, we observed other differences among the parasitoid species. For instance, P. coffea were more attracted to 490–540 nm than to 570 nm, whereas the bethylids did not discriminate between 490–540 nm or 570 nm. Our results are discussed in relation to possible implications associated with the vision of these parasitoid species.
Effect of Winning Experience on Aggression Involving Dangerous Fighting Behavior in Anastatus disparis (Hymenoptera: Eupelmidae)
Authors: Liu Peng-Cheng , Hao De-Jun , Hu Hao-Yuan , Wei Jian-Rong , Wu Fan , Shen Jie , Xu Shen-jia , Xie Qi-Yue
Published: April 2020

Aggressive behavior is widely observed in animal species for acquiring important resources and usually includes both dangerous and nondangerous fighting patterns. Only a few species show dangerous fighting patterns that are defined by fights ending with contestants being severely injured or killed. Prior experience, an important factor in many species, has been demonstrated to affect a contestant’s subsequent fighting behavior. Few studies have focused on the effect of experience on aggression involving dangerous fighting patterns. Here, an egg parasitoid wasp, Anastatus disparis, which shows extreme and dangerous fighting behavior to acquire mating opportunities, was used as an experimental model. Our results showed that the fighting intensity of the winning males significantly decreased subsequent fighting behavior, which was inconsistent with general predictions. Transcriptomic analyses showed that many genes related to energy metabolism were downregulated in winners, and winners increased their fighting intensity after dietary supplementation. Our study suggested that fighting in A. disparis is a tremendous drain on energy. Thus, although males won at combat, significant reductions in available energy constrained the intensity of subsequent fights and influenced strategic decisions. In addition, winners might improve their fighting skills and abilities from previous contests, and their fighting intensity after dietary supplementation was significantly higher than that of males without any fighting experience. Generally, in A. disparis, although winners increased their fighting ability with previous experience, the available energy in winners was likely to be a crucial factor affecting the intensity and strategic decisions in subsequent fights.
Oral and Topical Insecticide Response Bioassays and Associated Statistical Analyses Used Commonly in Veterinary and Medical Entomology
Authors: Burgess Edwin R , King Bethia H , Geden Christopher J
Published: April 2020

Veterinary and medical entomologists who are involved in research on pest control often need to perform dose–response bioassays and analyze the results. This article is meant as a beginner’s guide for doing this and includes instructions for using the free program R for the analyses. The bioassays and analyses are described using previously unpublished data from bioassays on house flies, Musca domestica Linnaeus (Diptera: Muscidae), but can be used on a wide range of pest species. Flies were exposed topically to beta-cyfluthrin, a pyrethroid, or exposed to spinosad or spinetoram in sugar to encourage consumption. LD50 values for beta-cyfluthrin in a susceptible strain were similar regardless of whether mortality was assessed at 24 or 48 h, consistent with it being a relatively quick-acting insecticide. Based on LC50 values, spinetoram was about twice as toxic as spinosad in a susceptible strain, suggesting a benefit to formulating spinetoram for house fly control, although spinetoram was no more toxic than spinosad for a pyrethroid-resistant strain. Results were consistent with previous reports of spinosad exhibiting little cross-resistance. For both spinosad and spinetoram, LC50 values were not greatly different between the pyrethroid-resistant strain and the susceptible strain.
Rickettsia spp. in Five Tick Species Collected in Central California
Authors: Osborne Cameron J , Wakeman-Hill Alya J , Loa Steven E , Crosbie Paul R , Van Laar Tricia A
Published: April 2020

Tick-borne disease surveillance in North America has long focused on Lyme disease, though there is currently a significant shift towards comprehensive pathogen surveillance in ticks. Central California has often been overlooked in regular tick-borne pathogen surveillance despite the presence of numerous medically important tick species. The bacterial genus Rickettsia contains tick-borne species that are known pathogens, such as those in the spotted fever group; nonpathogenic endosymbionts; and many species with unknown pathogenic potential. Five common tick species (Ixodes pacificus Cooley and Kohls [Acari: Ixodidae], Dermacentor occidentalis Marx [Acari: Ixodidae], D. variabilis Say, Rhipicephalus sanguineus Latreille [Acari: Ixodidae], and Ornithodoros parkeri Cooley [Acari: Argasidae]) of California were collected by both traditional and modern techniques, and subsequently screened for Rickettsia spp. Many individuals from all five tick species were PCR positive for Rickettsia spp., and a combination of species-specific primers, a restriction fragment length polymorphism assay, and DNA sequencing was used to further characterize the species composition in these ticks. Probable Rickettsia philipii (Rickettsia 364D) was detected in one (1.56%) D. occidentalis collected in Fresno County; R. rhipicephali was detected in 23.4% of D. occidentalis from Fresno Co.; R. bellii was detected in 88.2% of D. variabilis, 7.8% of D. occidentalis, and in one R. rhipicephalus (1.1%) from Fresno Co.; R. monacensis str. Humboldt was detected in three (100%) of I. pacificus collected in both Fresno and Madera Co.; and an uncharacterized Rickettsia was detected in (26.4%) of O. parkeri collected in both Fresno and Madera Co. The findings in this study highlight the need for ongoing surveillance in this region of California.
Postcolonial Reflexivity in the News Industry: The Case of Foreign Correspondents in Kenya and South Africa
Authors: Nothias Toussaint
Published: April 2020

Over the past 30 years, scholars have criticized the propensity of the international media to reproduce damaging and racist stereotypes about Africa. How do foreign correspondents, who are key actors in the production of Africa's media image, position themselves in relation to this criticism? Based on 35 interviews conducted with correspondents in Kenya and South Africa between 2013 and 2017, I find that many correspondents recognize the negative contributions of the news industry to representational Othering, thereby agreeing with the general tenets of the criticism. This paper is an in-depth exploration of this phenomenon, which I call postcolonial reflexivity. I outline the features of this postcolonial reflexivity, discuss its impact on journalistic practices, and explore the reasons for its prevalence among correspondents. Overall, the research contributes to bridging the gap between the textual orientation of postcolonial studies and the inclination for analyzing production practices in journalism studies.
Vacuolar sucrose homeostasis is critical for plant development, seed properties, and night-time survival in Arabidopsis
Authors: Vu Duc Phuong , Martins Rodrigues Cristina , Jung Benjamin , Meissner Garvin , Klemens Patrick A W , Holtgräwe Daniela , Fürtauer Lisa , Nägele Thomas , Nieberl Petra , Pommerrenig Benjamin , Neuhaus H Ekkehard
Published: April 2020

Most cellular sucrose is present in the cytosol and vacuoles of plant cells; however, little is known about the effect of this sucrose compartmentation on plant properties. Here, we examined the effects of altered intracellular sucrose compartmentation in Arabidopsis thaliana leaves by heterologously expressing the sugar beet (Beta vulgaris) vacuolar sucrose loader BvTST2.1 and by generating lines with reduced vacuolar invertase activity (amiR vi1-2). Heterologous expression of BvTST2.1 led to increased monosaccharide levels in leaves, whereas sucrose levels remained constant, indicating that vacuolar invertase activity in mesophyll vacuoles exceeds sucrose uptake. This notion was supported by analysis of tobacco (Nicotiana benthamiana) leaves transiently expressing BvTST2.1 and the invertase inhibitor NbVIF. However, sucrose levels were strongly elevated in leaf extracts from amiR vi1-2 lines, and experiments confirmed that sucrose accumulated in the corresponding vacuoles. The amiR vi1-2 lines exhibited impaired early development and reduced seed weight. When germinated in the dark, amiR vi1-2 seedlings were less able to convert sucrose into monosaccharides than the wild type. Cold temperatures strongly down-regulated both VI genes, but the amiR vi1-2 lines showed normal frost tolerance. These observations indicate that increased vacuolar sucrose levels fully compensate for the effects of low monosaccharide concentrations on frost tolerance.
A transition from parabolic to conical shape as a common effect in nearby AGN jets
Authors: Kovalev Y Y , Pushkarev A B , Nokhrina E E , Plavin A V , Beskin V S , Chernoglazov A V , Lister M L , Savolainen T
Published: April 2020

Observational studies of collimation in jets in active galactic nuclei (AGN) are a key to understanding their formation and acceleration processes. We have performed an automated search for jet shape transitions in a sample of 367 AGN using VLBA data at 15 and 1.4 GHz. This search has found 10 out of 29 nearby jets at redshifts z < 0.07 with a transition from a parabolic to conical shape, while the full analysed sample is dominated by distant AGN with a typical z ≈ 1. The ten AGN are UGC 00773, NGC 1052, 3C 111, 3C 120, TXS 0815−094, Mrk 180, PKS 1514+00, NGC 6251, 3C 371, and BL Lac. We conclude that the geometry transition may be a common effect in AGN jets. It can be observed only when sufficient linear resolution is obtained. Supplementing these results with previously reported shape breaks in the nearby AGN 1H 0323+342 and M87, we estimate that the break occurs at 105–106 gravitational radii from the nucleus. We suggest that the jet shape transition happens when the bulk plasma kinetic energy flux becomes equal to the Poynting energy flux, while the ambient medium pressure is assumed to be governed by Bondi accretion. In general, the break point may not coincide with the Bondi radius. The observational data support our model predictions on the jet acceleration and properties of the break point.
Bounds on WIMP dark matter from galaxy clusters at low redshift
Authors: Tan Xiuhui , Colavincenzo Manuel , Ammazzalorso Simone
Published: April 2020

In this work we study the cross-correlation between Fermi-LAT diffuse γ-ray maps and galaxy cluster catalogues in order to search for a diffuse dark matter signal. We employ an accurate estimation of the error covariance matrix and we select 4 galaxy cluster catalogues at low-redshift 0 < z < 0.2 with large-halo mass M500 > 1013M⊙ including infrared, optical and X-rays wavebands. We consider weakly interacting massive particle (WIMP) dark matter with 100% branching ratios into τ+τ−, $b\bar{b}$, W+W− and μ+μ−. No evidence for a WIMP dark matter signal is identified, and we derive competitive bounds. The WIMP thermally averaged cross section is excluded at 95% C.L. for dark matter masses below 20 GeV and annihilation in the τ+τ− channel.
A census of main-sequence interactions in the Multiple Star Catalogue
Authors: Hamers Adrian S
Published: April 2020

Statistics of hierarchical systems containing three or more stars are continuously improving. The Multiple Star Catalogue (MSC) is currently the most comprehensive catalogue of multiple-star systems and contains component masses, orbital periods, and additional information. The systems in the MSC are interesting for several reasons, including the long-term dynamical evolution of few-body systems. Although the secular evolution of triples and quadruples has been explored before, a systematic study of the systems in the MSC including also quintuples and sextuples has not been carried out. Here, we explore the main-sequence (MS) evolution of stars from the MSC based on approximately 2 × 105 secular dynamical integrations. We estimate statistical probabilities for strong interactions during the MS such as tidal evolution and mass transfer, and the onset of dynamical instability. Depending on the assumed model for the unknown orbital elements, we find that the fraction of non-interacting systems is largest for triples (∼0.9), and decreases to ∼0.6–0.8 for sextuples. The fraction of strong interactions increases from ∼0.1 to ∼0.2 from triples to sextuples, and the fraction of dynamically unstable systems increases from ∼0.001 to ∼0.1–0.2. The larger fractions of strong interactions and dynamical instability in systems with increasing multiplicity can be attributed to increasingly complex secular evolution in these systems. Our results indicate that a significant fraction of high-multiplicity systems interact or become dynamically unstable already during the MS, with an increasing importance as the number of stars increases.
Statistics and properties of emission-line regions in the local volume dwarf galaxies
Authors: Karachentsev I D , Kaisin S S
Published: April 2020

We used the H α images  from a large sample of nearby late-type dwarf galaxies to investigate properties of their emission structure. The sample consists of 300 galaxies of the irregular (Irr), Magellanic irregular (Im), blue compact dwarf (BCD), and transition (Tr) types situated within a distance of 11 Mpc. In each galaxy, we indicated the number of compact H ii regions, the presence of bubble-like or filament-like structures, the presence of a faint diffuse emission, and a sign of the global burst. The larger the luminosity of a galaxy, the greater number of compact H ii sources in it. The integral and specific star formation rates (SFRs) of the dwarf increase steeply with the increase of the number of H ii regions showing the evidence of the epidemic character of the star formation process. The dwarf galaxies with emission-line bubbles, or filaments, or signs of the global star formation burst have approximately the same hydrogen-mass-to-luminosity ratio as that of the whole sample objects. However, their mean SFR is significantly higher than that of other galaxies in the sample. Emission bubble-like structures are found in the nearby dwarfs with a frequency of one case per four to five galaxies. Their linear diameters are close to those expected for supernova remnants. The mean specific SFR for the nearby late-type dwarfs is close to the Hubble parameter, H0 = −10.14 dex (yr)−1, consistent with the sluggish cosmic star formation history of galaxies of this kind.
Refracting into ultra-diffuse galaxy NGC 1052-DF2 by passing near the centre of NGC 1052
Authors: Huo Ran
Published: April 2020

The recent observation of the ultra-diffuse galaxy NGC 1052-DF2 shows a galaxy may lack dark matter, which becomes a challenge to the standard structure formation theory. Here, we show that such phenomena can be a natural consequence if the NGC 1052-DF2 had experienced a single passage within a few kpc to the centre of the galaxy NGC 1052. The tidal effect of NGC 1052 in the encounter will heat the NGC 1052-DF2, and stretch the previous dwarf galaxy significantly into its current size. The relative lack of dark matter in the observed region is a natural consequence of the dark matter limited total amount in the corresponding small central region before the encounter, together with a systematic underestimation of the trace mass estimator method during revirialization after the encounter. The observed flat distribution of the ultra-diffuse galaxy can be reproduced with a cored initial star profile, which is a major improvement compared with the previous work. Our results show no need for introducing any new physical mechanism, as well as an alternative origin of an ultra-diffuse galaxy without repeated pericentre passage.
Artefacts of circumpolar cartography in radio pulsar polarization
Authors: Dyks J
Published: April 2020

Single-pulse data on radio pulsar polarization are traditionally presented in the form of two-dimensional grey-scale patterns with the pulse longitude and polarization angle (PA) on the horizontal and vertical axis, respectively. Such diagrams reveal several enigmatic polarization effects: (1) bifurcations and loops of PA curve under central pulse components, (2) vertical spread of flux at all PA values, (3) exchange of power content between PA tracks of two orthogonal polarization modes (OPMs), and (4) peripherically flat PA swings that span more than 180 deg. It is shown that all these phenomena result from passage of observed polarization state near the pure-V pole of Poincaré sphere. Much of their complexity results from cartographic transformation from Poincaré sphere to the longitude–PA plane. An odd number of near-pole passages produce apparent replacement of OPM power in the profile wings, although the same amount of flux keeps staying in each modal patch on the Poincaré sphere. The fitting of pulsar PA curves should therefore allow for transitions between the primary (strong) and secondary (weak) PA tracks. The Stokes space (or Poincaré sphere) representation of pulsar polarization data contains crucial polarization information and needs to accompany the traditional viewing if the published figures are to be fully useful for interpretation.
Discovering the most elusive radio relic in the sky: diffuse shock acceleration caught in the act?
Authors: Locatelli Nicola T , Rajpurohit Kamlesh , Vazza Franco , Gastaldello Fabio , Dallacasa Daniele , Bonafede Annalisa , Rossetti Mariachiara , Stuardi Chiara , Bonassieux Etienne , Brunetti Gianfranco , Brüggen Marcus , Shimwell Timothy
Published: April 2020

The origin of radio relics is usually explained via diffusive shock acceleration (DSA) or re-acceleration of electrons at/from merger shocks in galaxy clusters. The case of acceleration is challenged by the low predicted efficiency of low Mach number merger shocks, unable to explain the power observed in most radio relics. In this letter, we present the discovery of a new giant radio relic around the galaxy cluster Abell 2249 ($z$ = 0.0838) using Low-Frequency Array (LOFAR). It is special since it has the lowest surface brightness of all known radio relics. We study its radio and X-ray properties combining LOFAR data with uGMRT, JVLA, and XMM. This object has a total power of $L_{1.4\rm\, GHz}=4.1\pm 0.8 \times 10^{23}$ W Hz−1 and integrated spectral index α = 1.15 ± 0.23. We infer for this radio relic a lower bound on the magnetization of $B\ge 0.4\, \mu$G, a shock Mach number of $\mathcal {M}\approx 3.79$, and a low acceleration efficiency consistent with DSA. This result suggests that a missing population of relics may become visible, thanks to the unprecedented sensitivity of the new generation of radio telescopes.
Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos
Authors: Shafee Thomas , Bacic Antony , Johnson Kim
Published: April 2020

Approaches for studying the evolution of globular proteins are now well established yet are unsuitable for disordered sequences. Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence–function relationships. Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these. We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.

Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains. Clusters can be similarly identified in the seemingly random Pro-rich AG and Pro-depleted disordered regions. Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs. We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features. Mapping these features onto the functionally characterized FLAs therefore enables their sequence–function relationships to be interrogated. These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
Pervasive Phenotypic Impact of a Large Nonrecombining Introgressed Region in Yeast
Authors: Brion Christian , Caradec Claudia , Pflieger David , Friedrich Anne , Schacherer Joseph
Published: April 2020

To explore the origin of the diversity observed in natural populations, many studies have investigated the relationship between genotype and phenotype. In yeast species, especially in Saccharomyces cerevisiae, these studies are mainly conducted using recombinant offspring derived from two genetically diverse isolates, allowing to define the phenotypic effect of genetic variants. However, large genomic variants such as interspecies introgressions are usually overlooked even if they are known to modify the genotype–phenotype relationship. To have a better insight into the overall phenotypic impact of introgressions, we took advantage of the presence of a 1-Mb introgressed region, which lacks recombination and contains the mating-type determinant in the Lachancea kluyveri budding yeast. By performing linkage mapping analyses in this species, we identified a total of 89 loci affecting growth fitness in a large number of conditions and 2,187 loci affecting gene expression mostly grouped into two major hotspots, one being the introgressed region carrying the mating-type locus. Because of the absence of recombination, our results highlight the presence of a sexual dimorphism in a budding yeast for the first time. Overall, by describing the phenotype–genotype relationship in the Lachancea kluyveri species, we expanded our knowledge on how genetic characteristics of large introgression events can affect the phenotypic landscape.