bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Linseed (Linum usitatissimum) Groats-Based Mixed Feed Supplements on Diet Nutrient Digestibility and Blood Parameters of Horses
Authors: Saastamoinen Markku , Särkijärvi Susanna
Published: February 2020

Linseed (Linum usitatissimum) and its by-products are common supplements used in equine diets and are claimed to have beneficial health effects. In this study, the effect of linseed groat-based fibrous feed supplements on diet digestibility was studied. Also, possible detrimental health effects due to continuous feeding of supplemental feeds containing linseed were examined by evaluating blood parameters. The experimental design was arranged as two balanced 3 × 3 Latin Squares. The horses were individually fed at the maintenance energy level, the forage-to-concentrate ratio being 70:30, with three diets: (A) Control diet consisting of dried hay and whole oats; (B) Control diet + Feed 1; and (C) Control diet + Feed 2. Feed 1 contained 70% of linseed groats, 15% dried carrot, 10% dried garlic and 5% molasses. Feed 2 contained 65% linseed groats, 15% molassed sugar-beet pulp, 10% dried garlic, 5% dried carrot and 5% molasses. Digestibility data were obtained by using chromium mordanted straw as an indigestible external marker for the estimation of apparent digestibility. Blood samples were collected from the jugular vein at the end of each feeding period to evaluate the possible effects of the supplemented diets B and C on the health of the horses. Diets B and C had a higher digestibility of crude protein compared to the control diet A (p < 0.05). In addition, the digestibility of ether extract was higher in the supplemented diets than in the basal feeding (p < 0.01). There were no statistically significant differences or trends (p > 0.05) in the blood parameters between the treatments. It is concluded that linseed groat-based supplements (offering approximately 6.3%–6.7% linseed groats in the diet’s dry matter (DM), or 0.8 g/kg BW/d), and feed containing soluble fibre sources (sugar-beet pulp, dehydrated carrot), improved the crude protein and fat digestibility of hay-oats diets of horses, and can be used, for example, in feeding strategies replacing grains in the horse rations in order to reduce the intake of starch without any adverse effects on the blood parameters and health of the horses.
Ashwin Gene Expression Profiles in Oocytes, Preimplantation Embryos, and Fetal and Adult Bovine Tissues
Authors: Moreno-Brito Verónica , Morales-Adame Daniel , Soto-Orduño Elier , González-Chávez Susana Aideé , Pacheco-Tena César , Espino-Solis Gerardo Pavel , Leal-Berumen Irene , González-Rodríguez Everardo
Published: February 2020

The ashwin gene, originally identified in Xenopus laevis, was found to be expressed first in the neural plate and later in the embryonic brain, eyes, and spinal cord. Functional studies of ashwin suggest that it participates in cell survival and anteroposterior patterning. Furthermore, ashwin is expressed zygotically in this species, which suggests that it participates in embryonic development. Nevertheless, the expression of this gene has not been studied in mammals. Thus, the aim of this study was to analyze the ashwin expression pattern in bovine fetal and adult tissues, as well as in three independent samples of immature and mature oocytes, and in two- to four-, and eight-cell embryos, morula, and blastocysts. Spatiotemporal expression was analyzed using real-time polymerase chain reaction (PCR); ashwin mRNA was detected in all tissues analyzed, immature and mature oocytes, and two- to eight-cell embryos. It was down-regulated in morula and blastocysts, suggesting that this expression profile is similar to that of maternal genes. Immunohistochemical localization of the ashwin protein in fetal and adult ovaries and testes reveals that this protein is consistently present during all stages of follicular development and during bovine spermatogenesis. These observations lead us to propose ashwin as an important gene involved in mammalian reproduction.
Blob Detection and Deep Learning for Leukemic Blood Image Analysis
Authors: Di Ruberto Cecilia , Loddo Andrea , Puglisi Giovanni
Published: February 2020

In microscopy, laboratory tests make use of cell counters or flow cytometers to perform tests on blood cells, like the complete blood count, rapidly. However, a manual blood smear examination is still needed to verify the counter results and to monitor patients under therapy. Moreover, the manual inspection permits the description of the cells’ appearance, as well as any abnormalities. Unfortunately, manual analysis is long and tedious, and its result can be subjective and error-prone. Nevertheless, using image processing techniques, it is possible to automate the entire workflow, both reducing the operators’ workload and improving the diagnosis results. In this paper, we propose a novel method for recognizing white blood cells from microscopic blood images and classify them as healthy or affected by leukemia. The presented system is tested on public datasets for leukemia detection, the SMC-IDB, the IUMS-IDB, and the ALL-IDB. The results are promising, achieving 100% accuracy for the first two datasets and 99.7% for the ALL-IDB in white cells detection and 94.1% in leukemia classification, outperforming the state-of-the-art.
Experimental Study on Dynamic Compression Mechanical Properties of Aluminum Honeycomb Structures
Authors: Zhang Sheng , Chen Wei , Gao Deping , Xiao Liping , Han Longbao
Published: February 2020

In this paper, dynamic compression tests are developed to investigate the dynamic compression mechanical properties of the aluminum honeycomb structures at different strain rates, especially at the high strain rates. The difficulties at the high strain rates exist due to the large deformation, the low wave resistance and the size effect of the honeycomb structures. The Split Hopkinson Pressure Bar (SPHB) test method is carried out and special measures such as the adoption of waveform shaper, the size optimization of the impact bar and the specimen, and employment of the semiconductor strain gauge, etc. are taken to overcome the difficulties. It is discovered that the dynamic compression mechanical properties possess a stress hardening effect at a high strain rate from 1.3 × 103 s−1 to 2.0 × 103 s−1, but then a stress softening effect at a high strain rate of 4.6 × 103 s−1. It is also discovered that the yield strength and the average plateau stress at the strain rate of 2.0 × 103 s−1 is higher than that at the strain rate of 1.3 × 103 s−1. However, the yield strength and the average plateau stress at the strain rate of 4.6 × 103 s−1 is lower than that at the strain rate of 2.0 × 103 s−1 and 1.3 × 103 s−1, but higher than that at a quasi-static state. This indicates that the aluminum honeycomb structure is sensitive to the strain rate. Additionally, the damage mode of the aluminum honeycomb structure is plastic buckling, collapse and folding of the cell wall, which is carried out using dynamic compression tests. The folding length of the cell wall at a higher strain rate is found to be longer than that at a lower strain rate. The test results can also be used as the stress–strain curves of the honeycomb constitutive model at the high strain rates to carry out the numerical simulation of high-speed impact.
Regressed Terrain Traversability Cost for Autonomous Navigation Based on Image Textures
Authors: Bekhti Mohammed Abdessamad , Kobayashi Yuichi
Published: February 2020

The exploration of remote, unknown, rough environments by autonomous robots strongly depends on the ability of the on-board system to build an accurate predictor of terrain traversability. Terrain traversability prediction can be made more cost efficient by using texture information of 2D images obtained by a monocular camera. In cases where the robot is required to operate on a variety of terrains, it is important to consider that terrains sometimes contain spiky objects that appear as non-uniform in the texture of terrain images. This paper presents an approach to estimate the terrain traversability cost based on terrain non-uniformity detection (TNUD). Terrain images undergo a multiscale analysis to determine whether a terrain is uniform or non-uniform. Terrains are represented using a texture and a motion feature computed from terrain images and acceleration signal, respectively. Both features are then combined to learn independent Gaussian Process (GP) predictors, and consequently, predict vibrations using only image texture features. The proposed approach outperforms conventional methods relying only on image features without utilizing TNUD.
Research on Optimized Product Image Design Integrated Decision System Based on Kansei Engineering
Authors: Xue Lei , Yi Xiao , Zhang Ye
Published: February 2020

In order to facilitate the development of product image design, the research proposes the optimized product image design integrated decision system based on Kansei Engineering experiment. The system consists of two sub-models, namely product image design qualitative decision model and quantitative decision model. Firstly, using the product image design qualitative decision model, the influential design elements for the product image are identified based on Quantification Theory Type I. Secondly, the quantitative decision model is utilized to predict the product total image. Grey Relation Analysis (GRA)–Fuzzy logic sub-models of influential design elements are built up separately. After that, utility optimization model is applied to obtain the multi-objective product image. Finally, the product image design integrated decision system is completed to optimize the product image design in the process of product design. A case study of train seat design is given to demonstrate the analysis results. The train seat image design integrated decision system is constructed to determine the product image. This shows the proposed system is effective and for predicting and evaluating the product image. The results provide meaningful improvement for product image design decision.
TiO2 Nanostructured Films for Electrochromic Paper Based-Devices
Authors: Nunes Daniela , Freire Tomas , Barranger Andrea , Vieira João , Matias Mariana , Pereira Sonia , Pimentel Ana , Cordeiro Neusmar J. A. , Fortunato Elvira , Martins Rodrigo
Published: February 2020

Electrochromic titanium dioxide (TiO2) nanostructured films were grown on gold coated papers using a microwave-assisted hydrothermal method at low temperature (80 °C). Uniform nanostructured films fully covered the paper substrate, while maintaining its flexibility. Three acids, i.e., acetic, hydrochloric and nitric acids, were tested during syntheses, which determined the final structure of the produced films, and consequently their electrochromic behavior. The structural characteristics of nanostructured films were correlated with electrochemical response and reflectance modulation when immersed in 1 M LiClO4-PC (lithium perchlorate with propylene carbonate) electrolyte, nevertheless the material synthesized with nitric acid resulted in highly porous anatase films with enhanced electrochromic performance. The TiO2 films revealed a notable contrast behavior, reaching for the nitric-based film optical modulations of 57%, 9% and 22% between colored and bleached states, at 250, 550 and 850 nm, respectively in reflectance mode. High cycling stability was also obtained performing up to 1500 cycles without significant loss of the electrochromic behavior for the nitric acid material. The approach developed in this work proves the high stability and durability of such devices, together with the use of paper as substrate that aggregates the environmentally friendly, lightweight, flexibility and recyclability characters of the substrate to the microwave synthesis features, i.e., simplicity, celerity and enhanced efficiency/cost balance.
Broadband Generalized Sidelobe Canceler Beamforming Applied to Ultrasonic Imaging
Authors: Li Jiake , Ma Zhe , Mao Lei , Wang Zhengjun , Wang Yi , Cai Huaiyu , Chen Xiaodong
Published: February 2020

A broadband generalized sidelobe canceler (Broadband-GSC) application for near-field beamforming is proposed. This approach is implemented in the wavelet domain. Broadband-GSC provides a set of complex, adapted apodization weights for each wavelet subband. The proposed method constrains interference and noise signal to improve the lateral resolution with only one single emission. Performance of the proposed beamforming is tested on simulated data obtained with Field II. Experiments have proved that the new beamforming can significantly increase the image quality compared with delay-and-sum (DAS) and synthetic aperture (SA). Imaging of scattering points show that Broadband-GSC improves the lateral resolution by 43.2% and 58.0% compared with SA and DAS, respectively. Meanwhile，Broadband-GSC reduces the peak sidelobe level by 11.6 dB and 26.4 dB compared with SA and DAS, respectively. Plane wave emission experiment indicates that Broadband-GSC can improve the lateral resolution by 44.2% compared with DAS. Furthermore, the new beamforming introduces the possibility for higher frame-rate and higher investigation depth with increased lateral resolution.
Angiographic Imaging of an In Vivo Mouse Brain as a Guiding Star for Automatic Digital Refocusing in OCT
Authors: Park Kwan Seob , Kim Ju Wan , Lee Byeong Ha , Eom Tae Joong
Published: February 2020

A method allows the extraction of the recovery factor that maximizes the image contrast of OCT (optical coherence tomography) and/or OCTA (OCT angiography) of a living subject is proposed in this study. Due to the finite depth of focus in imaging optics, the volume OCT imaging suffers from blurriness in the lateral resolution. By utilizing the digital hologram method or angular spectrum method, the blurred image can be refocused. However, for in vivo OCT imaging, evaluation of the image focus is not easy, owing to the cloudy structure of the brain. In the proposed method, the blood flow signals were used as a guiding star to find the recovery factor. The propagation distance to a focal plane was automatically determined by evaluating the contrast of a cross-sectional OCTA image. The performance was examined though in vivo mouse brain OCT/OCTA imaging. The image singularity of the blood flow in OCTA was very effective at evaluating the contrast of the image.
Rapid Efficacy of Gemtuzumab Ozogamicin in Refractory AML Patients with Pulmonary and Kidney Failure
Authors: Zaytsev Daniil , Girshova Larisa , Ivanov Vladimir , Budaeva Irina , Motorin Dmitri , Badaev Renat , Mirolubova Julia , Grobovenko Evgeni , Chitanava Tamara , Zaykova Ekaterina , Alexeeva Julia , Zaritskey Andrey
Published: February 2020

Objectives: To the best of our knowledge, data from Gemtuzumab ozogamicin in Acute Myeloid Leukemia (AML) patients with failure of organ functions and poor performance status are extremely lacking. Moreover, the fast recovery from organ failure, after Gemtuzumab ozogamicin administration, has never been reported. This study aimed to demonstrate the efficacy and rapid response of Gemtuzumab ozogamicin in refractory acute myeloid leukemia (AML) patients with pulmonary and kidney failure and poor performance status. Three refractory AML patients, with organ dysfunction, are described. One patient was pre-treated with intensive chemotherapy, and two other patients progressed during Azacitidine treatment. Two patients had respiratory failure grade 2 and one patient suffered from acute kidney insufficiency. Two patients were highly febrile with an elevated С-Reactive Protein (CRP) level. The WHO performance status of three was measured in all patients. Gemtuzumab ozogamicin administration was performed in three patients, followed by a further switch to Gemtuzumab ozogamicin + Azacitidine or “7+3” treatment. Results: Gemtuzumab ozogamicin administration resulted in abrupt fever cessation in two febrile patients simultaneously with a rapid decrease in CRP level and fast resolution of respiratory failure. Recovery of kidney function was noticed rapidly in patients with renal insufficiency. The WHO performance status was elevated in all three patients. No adverse grade II–III effects were noticed. Further treatment made two patients eligible for intensive chemotherapy, one patient underwent allogeneic stem cell transplantation, and the patient with kidney failure obtained complete remission. Conclusions: Gemtuzumab ozogamicin therapy appeared to be safe and highly efficacious in relapsed/refractory AML patients with organ dysfunction, like pulmonary or renal failure and poor performance status, and may contribute to rapid recovery from organ failures.
Role of an Aromatic–Aromatic Interaction in the Assembly and Trafficking of the Zebrafish Panx1a Membrane Channel
Authors: Timonina Ksenia , Kotova Anna , Zoidl Georg
Published: February 2020

Pannexin 1 (Panx1) is a ubiquitously expressed hexameric integral membrane protein known to function as an adenosine triphosphate (ATP) release channel. Panx1 proteins exist in unglycosylated core form (Gly0). They undergo critical post-translational modifications forming the high mannose glycosylation state (Gly1) in the endoplasmic reticulum (ER) and the complex glycosylation state (Gly2) in the Golgi apparatus. The regulation of transition from the ER to the cell membrane is not fully understood. Using site-specific mutagenesis, dye uptake assays, and interaction testing, we identified two conserved aromatic residues, Trp123 and Tyr205, in the transmembrane domains 2 and 3 of the zebrafish panx1a protein. Results suggest that both residues primarily govern the assembly of panx1a subunits into channels, with mutant proteins failing to interact. The results provide insight into a mechanism enabling regulation of Panx1 oligomerization, glycosylation, and trafficking.
Use of Titanium Complexes Bearing Diphenolate or Calix[n]arene Ligands in α-Olefin Polymerization and the ROP of Cyclic Esters
Authors: Santoro Orlando , Redshaw Carl
Published: February 2020

In this review, we discuss the use of titanium complexes bearing either bridged diphenolate or calix[n]arene (n = 4, 6, 8) ligation, in the formation of plastics from α-olefins or via the ring opening polymerization (ROP) of cyclic esters. The syntheses, molecular structures and catalytic behaviour of these systems are discussed, as well as where possible, the properties of the resultant polymers.
Preparation of Carbon-Based Solid Acid Catalysts Using Rice Straw Biomass and Their Application in Hydration of α-Pinene
Authors: Wei Zhaozhou , Xiong Deyuan , Duan Pengzhi , Ding Shilei , Li Yuanlin , Li Lisi , Niu Peirong , Chen Xusong
Published: February 2020

Carbon-based solid acid catalysts were prepared using rice straw (RS) waste, and the effects of carbonization temperature and sulfonation temperature on the catalytic activity were investigated. The properties of the catalysts were characterized using thermo gravimetric (TG), scanning electron microscope (SEM), Brunauer–Emmet–Teller (BET), Fourier transform infrared spectroscopy (FT-IR), temperature-programmed desorption (TPD), and X-ray photoelectron spectroscopy (XPS), and their activities were investigated through the hydration of α-pinene. The conversion of α-pinene and the selectivity of α-terpineol reached 67.60% and 57.07% at 80 °C and atmospheric pressure in 24 h, respectively. The high catalytic capacity of the catalyst is attributed to the high acid site density and high porosity of the catalyst. TPD analysis and FT-IR spectroscopy showed that the catalyst produced by low-temperature carbonization at 300 °C followed by low-temperature sulfonation at 80 °C had abundant strong acid sites (0.82 mmol/g), which can effectively inhibit the side reactions of hydrated α-pinene. The total acidity reached 2.87 mmol/g. N2-physisorption analysis clearly indicated that the obtained catalysts were mesopore-predominant materials, and the SBET and VTotal of catalysts reached 420.9 m2/g and 4.048 cm3/g, respectively. Preparation of the catalyst involves low energy consumption, and its cheap raw materials make the whole process simple, economical, and environmentally friendly.
A Novel Route for Agarooligosaccharide Production with the Neoagarooligosaccharide-Producing β-Agarase as Catalyst
Authors: Jiang Chengcheng , Liu Zhen , Sun Jianan , Xue Changhu , Mao Xiangzhao
Published: February 2020

Enzymes are catalysts with high specificity. Different compounds could be produced by different enzymes. In case of agaro-oligosaccharides, agarooligosaccharide (AOS) can be produced by α-agarase through cleaving the α-1,3-glycosidic linkages of agarose, while neoagarooligosaccharide (NAOS) can be produced by β-agarase through cleaving the β-1,4-glycosidic linkages of agarose. However, in this study, we showed that β-agarase could also be used to produce AOSs with high purity and yield. The feasibility of our route was confirmed by agarotriose (A3) and agaropentaose (A5) formation from agaroheptaose (A7) and agarononoses (A9) catalyzed by β-agarase. Agarose was firstly liquesced by citric acid into a mixture of AOSs. The AOSs mixture was further catalyzed by β-agarase. When using the neoagarotetraose-forming β-agarase AgWH50B, agarotriose could be produced with the yield of 48%. When using neoagarotetraose, neoagarohexaose-forming β-agarase DagA, both agarotriose and agaropentaose could be produced with the yield of 14% and 13%, respectively. Our method can be used to produce other value-added agaro-oligosaccharides from agarose by different agarolytic enzymes.
Future Therapeutic Directions for Smac-Mimetics
Authors: Morrish Emma , Brumatti Gabriela , Silke John
Published: February 2020

It is well accepted that the ability of cancer cells to circumvent the cell death program that untransformed cells are subject to helps promote tumor growth. Strategies designed to reinstate the cell death program in cancer cells have therefore been investigated for decades. Overexpression of members of the Inhibitor of APoptosis (IAP) protein family is one possible mechanism hindering the death of cancer cells. To promote cell death, drugs that mimic natural IAP antagonists, such as second mitochondria-derived activator of caspases (Smac/DIABLO) were developed. Smac-Mimetics (SMs) have entered clinical trials for hematological and solid cancers, unfortunately with variable and limited results so far. This review explores the use of SMs for the treatment of cancer, their potential to synergize with up-coming treatments and, finally, discusses the challenges and optimism facing this strategy.
Complex Mitochondrial Dysfunction Induced by TPP+-Gentisic Acid and Mitochondrial Translation Inhibition by Doxycycline Evokes Synergistic Lethality in Breast Cancer Cells
Authors: Fuentes-Retamal Sebastián , Sandoval-Acuña Cristian , Peredo-Silva Liliana , Guzmán-Rivera Daniela , Pavani Mario , Torrealba Natalia , Truksa Jaroslav , Castro-Castillo Vicente , Catalán Mabel , Kemmerling Ulrike , Urra Félix A. , Ferreira Jorge
Published: February 2020

The mitochondrion has emerged as a promising therapeutic target for novel cancer treatments because of its essential role in tumorigenesis and resistance to chemotherapy. Previously, we described a natural compound, 10-((2,5-dihydroxybenzoyl)oxy)decyl) triphenylphosphonium bromide (GA-TPP+C10), with a hydroquinone scaffold that selectively targets the mitochondria of breast cancer (BC) cells by binding to the triphenylphosphonium group as a chemical chaperone; however, the mechanism of action remains unclear. In this work, we showed that GA-TPP+C10 causes time-dependent complex inhibition of the mitochondrial bioenergetics of BC cells, characterized by (1) an initial phase of mitochondrial uptake with an uncoupling effect of oxidative phosphorylation, as previously reported, (2) inhibition of Complex I-dependent respiration, and (3) a late phase of mitochondrial accumulation with inhibition of α-ketoglutarate dehydrogenase complex (αKGDHC) activity. These events led to cell cycle arrest in the G1 phase and cell death at 24 and 48 h of exposure, and the cells were rescued by the addition of the cell-penetrating metabolic intermediates l-aspartic acid β-methyl ester (mAsp) and dimethyl α-ketoglutarate (dm-KG). In addition, this unexpected blocking of mitochondrial function triggered metabolic remodeling toward glycolysis, AMPK activation, increased expression of proliferator-activated receptor gamma coactivator 1-alpha (pgc1α) and electron transport chain (ETC) component-related genes encoded by mitochondrial DNA and downregulation of the uncoupling proteins ucp3 and ucp4, suggesting an AMPK-dependent prosurvival adaptive response in cancer cells. Consistent with this finding, we showed that inhibition of mitochondrial translation with doxycycline, a broad-spectrum antibiotic that inhibits the 28 S subunit of the mitochondrial ribosome, in the presence of GA-TPP+C10 significantly reduces the mt-CO1 and VDAC protein levels and the FCCP-stimulated maximal electron flux and promotes selective and synergistic cytotoxic effects on BC cells at 24 h of treatment. Based on our results, we propose that this combined strategy based on blockage of the adaptive response induced by mitochondrial bioenergetic inhibition may have therapeutic relevance in BC.
Shaping Pancreatic β-Cell Differentiation and Functioning: The Influence of Mechanotransduction
Authors: Alessandra Galli , Algerta Marku , Paola Marciani , Carsten Schulte , Cristina Lenardi , Paolo Milani , Elisa Maffioli , Gabriella Tedeschi , Carla Perego
Published: February 2020

Embryonic and pluripotent stem cells hold great promise in generating β-cells for both replacing medicine and novel therapeutic discoveries in diabetes mellitus. However, their differentiation in vitro is still inefficient, and functional studies reveal that most of these β-like cells still fail to fully mirror the adult β-cell physiology. For their proper growth and functioning, β-cells require a very specific environment, the islet niche, which provides a myriad of chemical and physical signals. While the nature and effects of chemical stimuli have been widely characterized, less is known about the mechanical signals. We here review the current status of knowledge of biophysical cues provided by the niche where β-cells normally live and differentiate, and we underline the possible machinery designated for mechanotransduction in β-cells. Although the regulatory mechanisms remain poorly understood, the analysis reveals that β-cells are equipped with all mechanosensors and signaling proteins actively involved in mechanotransduction in other cell types, and they respond to mechanical cues by changing their behavior. By engineering microenvironments mirroring the biophysical niche properties it is possible to elucidate the β-cell mechanotransductive-regulatory mechanisms and to harness them for the promotion of β-cell differentiation capacity in vitro.
Activation of Astroglial Connexin Is Involved in Concentration-Dependent Double-Edged Sword Clinical Action of Clozapine
Authors: Fukuyama Kouji , Okubo Ruri , Murata Masahiko , Shiroyama Takashi , Okada Motohiro
Published: February 2020

Clozapine (CLZ) is a gold-standard antipsychotic against treatment-refractory schizophrenia, but is one of the most toxic antipsychotic agents. Pharmacological mechanisms of the double-edged sword clinical action of CLZ remain to be clarified. To explore the mechanisms of CLZ, the present study determined the astroglial transmission associated with connexin43 (Cx43), which is the most principal expression in astrocytes and myocardial cells, and expression of Cx43 in primary cultured astrocytes. Both acute and subchronic administrations of CLZ concentration-dependently increased Cx43-associated astroglial release of l-glutamate and d-serine, whereas therapeutic-relevant concentration of CLZ acutely did not affect but subchronically increased astroglial release. In contrast, after the subchronic administration of therapeutic-relevant concentration of valproate (VPA), acute administration of therapeutic-relevant concentration of CLZ drastically increased Cx43-associated astroglial releases. VPA increased Cx43 expression in cytosol fraction without affecting plasma membrane fraction, whereas CLZ increased Cx43 expression in both fractions. Acute administration of therapeutic-relevant concentration of CLZ drastically increased Cx43 expression in the plasma membrane fraction of astrocytes subchronically treated with VPA. The present findings suggest that CLZ-induced the activation of Cx43-associated channel activity and transported Cx43 to plasma membrane, probably contribute to the double-edged sword clinical action of CLZ, such as improvement of cognitive dysfunction and CLZ-induced myocarditis.
Leveraging Blockchain Technology to Break the Cloud Computing Market Monopoly
Authors: Bakogiannis Tasos , Mytilinis Ioannis , Doka Katerina , Goumas Georgios
Published: February 2020

Cloud computing offerings traditionally originate from a handful of large and well established providers, which monopolize the market, preventing small players and individuals from having a share. As a result, the few, blindly and perforce trusted entities define the prices and manage to gain a significant competitive advantage by exploiting the knowledge derived by users’ data and computations. To tackle this monopoly and empower the democratization and full decentralization of the cloud computing market, we present CloudAgora, a platform that enables any potential resource provider, ranging from individuals to large companies, to monetize idle resources competing on equal terms, and allows any cloud consumer to enjoy access to low-cost storage and computation without having to trust any central authority. The key enabler of the platform is Blockchain technology, which is used to record commitment policies through the use of smart contracts, publicly verify off-chain services, both storage and computation related, and trigger automatic micropayments. On one hand, cloud consumers have the chance to request storage or compute resources, upload data, and outsource task processing over remote, fully distributed infrastructures. Although such infrastructures cannot be a priori trusted, CloudAgora offers mechanisms to ensure the verifiable validity of the outsourced storage and computation, discourage potential providers from behaving maliciously, and incentivize participants to play fair. On the other hand, providers are able to participate in auctions, placing bids for storage or computation tasks, serve requests, and offer validity proofs upon request. Our prototype is built as a Dapp on top of Ethereum and is available as an open source project.
In Situ Neutron Diffraction Study of Phase Transformation of High Mn Steel with Different Carbon Content
Authors: Kim Youngsu , Choi Wookjin , Choo Hahn , An Ke , Choi Ho-Suk , Lee Soo Yeol
Published: February 2020

In situ neutron diffraction was employed to examine the phase transformation behavior of high-Mn steels with different carbon contents (0.1, 0.3, and 0.5 wt.%C). With increasing carbon contents from 0.1 C to 0.5 C, the austenite phase fraction among the constituent phases increased from ~66% to ~98%, and stacking fault energy (SFE) increased from ~0.65 to ~16.5 mJ/m2. The 0.1 C and 0.3 C steels underwent phase transformation from γ-austenite to ε-martensite or α’-martensite during tensile deformation. On the other hand, the 0.5 C steel underwent phase transformation only from γ-austenite to ε-martensite. The 0.3 C steel exhibited a low yield strength, a high strain hardening rate, and the smallest elongation. The high strain hardening of the 0.3 C alloy was due to a rapid phase transformation rate from γ-austenite to ε-martensite. The austenite of 0.5 C steel was strengthened by mechanical twinning during loading process, and the twinning-induced plasticity (TWIP) effect resulted in a large ductility. The 0.5 wt.% carbon addition stabilized the austenite phase by delaying the onset of the ε-martensite phase transformation.
A Linear Regression Model for Determining the Pre-Exponential Factor and Interfacial Energy Based on the Metastable Zone Width Data
Authors: Shiau Lie-Ding
Published: February 2020

A linear regression model is presented in this study to determine the pre-exponential factor and interfacial energy of the crystallized substance based on classical nucleation theory using the metastable zone width data. The nucleation event is assumed corresponding to a point at which the total number density of the nuclei has reached a fixed (but unknown) value. One equation is derived for any temperature-dependent functional form of the solubility. Another equation is derived for the van’t Hoff solubility expression. The pre-exponential factor and interfacial energy obtained from these two equations are found consistent for the studied systems, including glutamic acid, glycine, and 3-nito-1,2,4-triazol-5-one. The results obtained from these two equations are also compared with those obtained from the integral method and classical 3D nucleation theory approach.
Diagnostic Dilemma of Recurrent Pulmonary Embolism
Authors: Dadarlat-Pop Alexandra , Burian Irina , Cadis Laura , Tomoaia Raluca , Oprea Alexandru
Published: February 2020

Popliteal venous aneurysms are rare vascular disorders associated with a high risk of pulmonary embolism. We present the case of a 56-year-old woman hospitalized for a third episode of unprovoked pulmonary embolism. Venous ultrasonography identified a popliteal aneurysm, repeatedly missed by two-point compression venous ultrasonography, which was eventually confirmed by a magnetic resonance examination. Because of its highly symptomatic nature despite optimal anticoagulant treatment, the decision was made to undergo surgery, consisting of aneurysmectomy followed by patch angioplasty. The goal of this paper is to report a rare case of popliteal venous aneurysm and its treatment strategies and postoperative evolution.
A Rare Case of Sigmoid Colon Carcinoma in Incarcerated Inguinal Hernia
Authors: Baldi Dario , Alfano Vincenzo , Punzo Bruna , Tramontano Liberatore , Baselice Simona , Spidalieri Gianluca , Micera Osvaldo , Cavaliere Carlo
Published: February 2020

Incarcerated inguinal hernia is a common diagnosis in patients presenting a painful and nonreducible groin mass. Although the diagnosis is usually made by physical examination, the content of the hernia sac and the extent of the surgical operation may vary and can require multimodal imaging integration (e.g., ultrasonography, computed tomography); the usual finding is a segment of small bowel and, less commonly, large bowel. We present an extremely rare case of a sigmoid cancer incarcerated in a left inguinal hernia and infiltrating the spermatic cord. The patient underwent whole-body computed tomography (CT) with contrast agent injection for staging, followed by a left hemicolectomy paralleled by a unilateral orchiectomy.
Assessing the Word Recognition Skills of German Elementary Students in Silent Reading—Psychometric Properties of an Item Pool to Generate Curriculum-Based Measurements
Authors: Voß Stefan , Blumenthal Yvonne
Published: February 2020

Given the high proportion of struggling readers in school and the long-term negative consequences of underachievement for those affected, the question of prevention options arises. The early identification of central indicators for reading literacy is a noteworthy starting point. In this context, curriculum-based measurements have established themselves as reliable and valid instruments for monitoring the progress of learning processes. This article is dedicated to the assessment of word recognition in silent reading as an indicator of adequate reading fluency. The process of developing an item pool is described, from which instruments for learning process diagnostics can be derived. A sample of 4268 students from grades 1–4 processed a subset of items. Each student template included anchor items, which all students processed. Using Item Response Theory, item statistics were estimated for the entire sample and all items. After eliminating unsuitable items (N = 206), a one-dimensional, homogeneous pool of items remained. In addition, there are high correlations with another established reading test. This provides the first evidence that the recording of word recognition skills for silent reading can be seen as an economic indicator for reading skills. Although the item pool forms an important basis for the extraction of curriculum-based measurements, further investigations to assess the diagnostic suitability (e.g., the measurement invariance over different test times) are still pending.
A Survey on the Micro-Phasor Measurement Unit in Distribution Networks
Authors: Dusabimana Emile , Yoon Sung-Guk
Published: February 2020

The Micro-Phasor Measurement Unit ( μ PMU) or distribution-level PMU (D-PMU) is a measurement device that measures the synchronized voltage and current values of electric power distribution networks. The synchronized data obtained by μ PMUs can be used for monitoring, diagnostic, and control distribution network applications, so that operators can understand the dynamic states of the distribution network in real-time. In this paper, we review the state-of-the-art μ PMU research which includes a list of μ PMU applications, monitoring and diagnostic functions, control applications, and optimal placement of the μ PMU. In addition, we analyze the benefits of μ PMUs in distribution networks; in particular, their reliability and resiliency, cost savings, and environmental and policy benefits.
Effects of Chickpea Protein on Carbohydrate Reactivity in Acrylamide Formation in Low Humidity Model Systems
Authors: Miśkiewicz Karolina , Rosicka-Kaczmarek Justyna , Nebesny Ewa
Published: February 2020

Asparagine and reducing sugars are the principal precursors of acrylamide in foods. Their main sources in pastries are flour and hen egg yolks. One method of reducing acrylamide content in food may be to add a chickpea protein preparation. The aim of the study was to determine the effects of the chickpea protein preparation on the thermodynamic properties of carbohydrates and the amount of acrylamide formed in low humidity model systems. In the studied systems, the type and amount of acrylamide precursors and humidity were designed to reflect the parameters typical of shortcrust cookies. In the study, the highest amounts of acrylamide were formed in the reaction between asparagine and fructose and the lowest in the reaction between asparagine and sucrose. Furthermore, the addition of chickpea protein to the analyzed carbohydrate–asparagine model systems reduced the content of acrylamide formed during baking at 180 °C regardless of the type of carbohydrate. The greatest acrylamide reduction (41%) was found in the model system containing fructose.
Erratum: Introduction to “Re-Mapping Cosmopolitanism”. Humanities 2019, 8, 127
Authors: Barker Jennifer , Zorn Christa
Published: February 2020

The authors would like to make the following changes to the published paper (Barker and Zorn 2019): [...]
Surgical Management of Spinal Disorders in People with Mucopolysaccharidoses
Authors: Terai Hidetomi , Nakamura Hiroaki
Published: February 2020

Mucopolysaccharidoses (MPS) are a group of inherited, multisystem, lysosomal storage disorders involving specific lysosomal enzyme deficiencies that result in the accumulation of glycosaminoglycans (GAG) secondary to insufficient degradation within cell lysosomes. GAG accumulation affects both primary bone formation and secondary bone growth, resulting in growth impairment. Typical spinal manifestations in MPS are atlantoaxial instability, thoracolumbar kyphosis/scoliosis, and cervical/lumbar spinal canal stenosis. Spinal disorders and their severity depend on the MPS type and may be related to disease activity. Enzyme replacement therapy or hematopoietic stem cell transplantation has advantages regarding soft tissues; however, these therapeutic modalities are not effective for bone or cartilage and MPS-related bone deformity including the spine. Because spinal disorders show the most serious deterioration among patients with MPS, spinal surgeries are required although they are challenging and associated with high anesthesia-related risks. The aim of this review article is to provide the current comprehensive knowledge of representative spinal disease in MPS and its surgical management, including the related pathology, symptoms, and examinations.
The Impact of the Circadian Genes CLOCK and ARNTL on Myocardial Infarction
Authors: Škrlec Ivana , Milić Jakov , Steiner Robert
Published: February 2020

The circadian rhythm regulates various physiological mechanisms, and its disruption can promote many disorders. Disturbance of endogenous circadian rhythms enhances the chance of myocardial infarction (MI), showing that circadian clock genes could have a crucial function in the onset of the disease. This case-control study was performed on 1057 participants. It was hypothesized that the polymorphisms of one nucleotide (SNP) in three circadian clock genes (CLOCK, ARNTL, and PER2) could be associated with MI. Statistically significant differences, estimated by the Chi-square test, were found in the distribution of alleles and genotypes between MI and no-MI groups of the CLOCK (rs6811520 and rs13124436) and ARNTL (rs3789327 and rs12363415) genes. According to the results of the present study, the polymorphisms in the CLOCK and ARNTL genes could be related to MI.
The Role of Periodontitis and Periodontal Bacteria in the Onset and Progression of Alzheimer’s Disease: A Systematic Review
Authors: Dioguardi Mario , Crincoli Vito , Laino Luigi , Alovisi Mario , Sovereto Diego , Mastrangelo Filiberto , Lo Russo Lucio , Lo Muzio Lorenzo
Published: February 2020

The evidence of a connection between the peripheral inflammatory processes and neurodegenerative diseases of the central nervous system is becoming more apparent. This review of the related literature highlights the most recent clinical, epidemiological, and in vitro studies trying to investigate possible connections between periodontal bacteria and the onset and progression of Alzheimer’s disease. This review was conducted by searching databases such as PubMed and Scopus using keywords or combinations such as Alzheimer’s Disease AND periodontal or dementia AND periodontitis OR periodontal. After eliminating overlaps and screening the articles not related to these issues, we identified 1088 records and proceeded to the selection of articles for an evaluation of the associative assumptions. The hypothesis suggested by the authors and confirmed by the literature is that the bacterial load and the inflammatory process linked to periodontal disease can intensify inflammation at the level of the central nervous system, favoring the occurrence of the disease. The analysis of the literature highlights how periodontal disease can directly contribute to the peripheral inflammatory environment by the introduction of periodontal or indirect pathogenic bacteria and proinflammatory cytokines locally produced at the periodontal level following bacterial colonization of periodontal defects.
Recurrence Patterns after Surgery in Patients with Different Endometriosis Subtypes: A Long-Term Hospital-Based Cohort Study
Authors: Nirgianakis Konstantinos , Ma Lijuan , McKinnon Brett , Mueller Michael D.
Published: February 2020

Recurrence of endometriosis after surgery constitutes a serious challenge. Whether there is an evolution of lesion subtypes with each recurrence and whether certain lesions subtypes tend to recur faster than others is not adequately addressed. Medical records of all patients who underwent surgery for endometriosis between 1997 and 2018 in the Department of Gynecology and Obstetrics, University of Bern, were reviewed. Inclusion criteria was surgically confirmed endometriosis recurrence, defined as a subsequent surgery for endometriosis after a previous complete surgical excision of endometriosis lesions. Three subtypes of endometriosis were defined: superficial peritoneal endometriosis (SUP), ovarian endometrioma (OMA), and deep infiltrating endometriosis (DIE). Time to recurrence and variation in endometriosis subtype between the first and recurrent surgeries were the primary outcome measures. Out of the 322 patients with recurrent surgery that were identified, for 234 of them, the endometriosis subtype at first surgery was confirmed and classified (SUP = 56, OMA = 124, DIE = 54). No statistically significant difference was found for time to recurrence between lesion subtypes. SUP compared to the other groups had a higher possibility of presenting with SUP at recurrence (Odds Ratio (OR): 3.65, 95% confidence interval (CI): 1.74–7.51) and OMA compared to the other groups had a higher possibility of presenting with OMA at recurrence (OR: 3.72, 95% CI: 2.04–6.74). Nevertheless, a large number of SUP patients subsequently presented with OMA (10/56: 17.9%) or DIE (27/56: 48.2%) lesions at recurrence. Similarly, a large number of OMA patients subsequently presented with DIE (49/124: 39.5%) lesions at recurrence. In conclusion, although SUP and OMA patients compared to the others are more likely to present with the same subtype at recurrence, increasing lesion subtype severity occurs in a substantial proportion of patients. Time to recurrence is independent from the lesion subtype at first surgery.
Describing Mining Tailing Flocculation in Seawater by Population Balance Models: Effect of Mixing Intensity
Authors: Quezada Gonzalo R. , Ayala Luís , Leiva Williams H. , Toro Norman , Toledo Pedro G. , Robles Pedro , I. Jeldres Ricardo
Published: February 2020

A population balance model (PBM) is used to describe flocculation of particle tailings in seawater at pH 8 for a range of mixing intensities. The size of the aggregates is represented by the mean chord length, determined by the focused beam reflectance measurement (FBRM) technique. The PBM follows the dynamics of aggregation and breakage processes underlying flocculation and provides a good approximation to the temporal evolution of aggregate size. The structure of the aggregates during flocculation is described by a constant or time-dependent fractal dimension. The results revealed that the compensations between the aggregation and breakage rates lead to a correct representation of the flocculation kinetics of the tailings of particles in seawater and, in addition, that the representation of the flocculation kinetics in optimal conditions is equally good with a constant or variable fractal dimension. The aggregation and breakage functions and their corresponding parameters are sensitive to the choice of the fractal dimension of the aggregates, whether constant or time dependent, however, under optimal conditions, a constant fractal dimension is sufficient. The model is robust and predictive with a few parameters and can be used to find the optimal flocculation conditions at different mixing intensities, and the optimal flocculation time can be used for a cost-effective evaluation of the quality of the flocculant used.