bluuebunny/crossref_metadata_2025 huggingface dataset:
Dehydration-Responsive Element Binding Protein 1C, 1E, and 1G Promote Stress Tolerance to Chilling, Heat, Drought, and Salt in Rice
Authors: Wang Huanhuan , Lu Shan , Guan Xiangyu , Jiang Yuan , Wang Bin , Hua Jian , Zou Baohong
Published: May 2022

The dehydration-responsive element binding protein 1 (DREB1)/C-repeat-binding factor (CBF) genes are key regulators of cold acclimation and freezing tolerance in the chilling tolerant Arabidopsis thaliana. Here, we investigated the function of three members of the 10 rice DREB1 genes, OsDREB1C, E, and G, in the chilling sensitive rice plants. Their loss of function (LOF) mutants were each more chilling susceptible compared to the wild type, and the LOF mutants of all three genes, dreb1ceg, were more chilling susceptible than any of the single mutants. Strikingly, these mutants were capable of cold acclimation, indicating that these rice DREB1 genes are important for basal chilling tolerance but not cold acclimation. Transcriptome and physiology analyses suggest that the OsDREB1C/E/G genes are involved in reactive oxygen species (ROS) scavenging and cell death regulation under chilling. Furthermore, these three rice DREB1 genes are found to promote tolerance to other abiotic stresses: the OsDREB1C/E/G genes are positive regulators of heat tolerance, OsDREB1C and OsDREB1G are positive regulators of salt tolerance, and OsDREB1G is a positive regulator of drought tolerance. These findings expand our knowledge of the roles of DREB1 proteins in plants, enhance our mechanistic understanding of abiotic stress tolerance and will facilitate the generation of stress-tolerant crop plants.
Genetic Diversity Analysis of Sapindus in China and Extraction of a Core Germplasm Collection Using EST-SSR Markers
Authors: Liu Jiming , Gao Shilun , Xu Yuanyuan , Wang Mianzhi , Ngiam Jia Jun , Rui Wen Nicholas Cho , Yi Joan Jong Jing , Weng Xuehuang , Jia Liming , Salojärvi Jarkko
Published: May 2022

Sapindus is an important forest tree genus with utilization in biodiesel, biomedicine, and it harbors great potential for biochemical engineering applications. For advanced breeding of Sapindus, it is necessary to evaluate the genetic diversity and construct a rationally designed core germplasm collection. In this study, the genetic diversity and population structure of Sapindus were conducted with 18 expressed sequence tag-simple sequence repeat (EST-SSR) markers in order to establish a core germplasm collection from 161 Sapindus accessions. The population of Sapindus showed high genetic diversity and significant population structure. Interspecific genetic variation was significantly higher than intraspecific variation in the Sapindus mukorossi, Sapindus delavayi, and combined Sapindus rarak plus Sapindus rarak var. velutinus populations. S. mukorossi had abundant genetic variation and showed a specific pattern of geographical variation, whereas S. delavayi, S. rarak, and S. rarak var. velutinus showed less intraspecific variation. A core germplasm collection was created that contained 40% of genetic variation in the initial population, comprising 53 S. mukorossi and nine S. delavayi lineages, as well as single representatives of S. rarak and S. rarak var. velutinus. These results provide a germplasm basis and theoretical rationale for the efficient management, conservation, and utilization of Sapindus, as well as genetic resources for joint genomics research in the future.
An Algorithm for Precipitation Correction in Flood Season Based on Dendritic Neural Network
Authors: Li Tao , Qiao Chenwei , Wang Lina , Chen Jie , Ren Yongjun
Published: May 2022

In recent years, the National Climate Center has developed a dynamic downscaling prediction technology based on the Climate-Weather Research and Forecasting (CWRF) regional climate model and used it for summer precipitation prediction, but there are certain deviations, and it is difficult to predict more accurately. The CWRF model simulates the summer precipitation forecast data from 1996 to 2019 and uses a combination of dendrite net (DD) and artificial neural networks (ANNs) to conduct a comparative analysis of summer precipitation correction techniques. While summarizing the characteristics and current situation of summer precipitation in the whole country, the meteorological elements related to precipitation are analyzed. CWRF is used to simulate summer precipitation and actual observation precipitation data to establish a model to correct the precipitation. By comparing with the measured data of the ground station after quality control, the relevant evaluation index analysis is used to determine the best revised model. The results show that the correction effect based on the dendritic neural network algorithm is better than the CWRF historical return, in which, the anomaly correlation coefficient (ACC) and the temporal correlation coefficient (TCC) both increased by 0.1, the mean square error (MSE) dropped by about 26%, and the overall trend anomaly (Ps) test score was also improved, showing that the machine learning algorithms can correct the summer precipitation in the CWRF regional climate model to a certain extent and improve the accuracy of weather forecasts.
Not Only Systemin: Prosystemin Harbors Other Active Regions Able to Protect Tomato Plants
Authors: Molisso Donata , Coppola Mariangela , Buonanno Martina , Di Lelio Ilaria , Aprile Anna Maria , Langella Emma , Rigano Maria Manuela , Francesca Silvana , Chiaiese Pasquale , Palmieri Gianna , Tatè Rosarita , Sinno Martina , Barra Eleonora , Becchimanzi Andrea , Monti Simona Maria , Pennacchio Francesco , Rao Rosa
Published: May 2022

Prosystemin is a 200-amino acid precursor expressed in Solanaceae plants which releases at the C-terminal part a peptidic hormone called Systemin in response to wounding and herbivore attack. We recently showed that Prosystemin is not only a mere scaffold of Systemin but, even when deprived of Systemin, is biologically active. These results, combined with recent discoveries that Prosystemin is an intrinsically disordered protein containing disordered regions within its sequence, prompted us to investigate the N-terminal portions of the precursor, which contribute to the greatest disorder within the sequence. To this aim, PS1-70 and PS1-120 were designed, produced, and structurally and functionally characterized. Both the fragments, which maintained their intrinsic disorder, were able to induce defense-related genes and to protect tomato plants against Botrytis cinerea and Spodoptera littoralis larvae. Intriguingly, the biological activity of each of the two N-terminal fragments and of Systemin is similar but not quite the same and does not show any toxicity on experimental non-targets considered. These regions account for different anti-stress activities conferred to tomato plants by their overexpression. The two N-terminal fragments identified in this study may represent new promising tools for sustainable crop protection.
Spatiotemporal Change of Net Primary Productivity and Its Response to Climate Change in Temperate Grasslands of China
Authors: Ma Rong , Xia Chunlin , Liu Yiwen , Wang Yanji , Zhang Jiaqi , Shen Xiangjin , Lu Xianguo , Jiang Ming
Published: May 2022

The temperate grasslands in China play a vital part in regulating regional carbon cycle and climate change. Net primary productivity (NPP) is a crucial index that reflects ecological function of plants and the carbon sequestration capacity of grassland ecosystem. Climate change can affect NPP by changing vegetation growth, but the effects of climate change on the NPP of China’s temperate grasslands remain unclear. Based on MODIS data and monthly climate data during 2000–2020, this study explored the spatiotemporal changes in grassland NPP and its response to climate change in temperate grasslands of China. We found that the annual NPP over the entire China’s temperate grasslands increased significantly by 4.0 gC/m2/year from 2000 to 2020. The annual NPP showed increasing trends for all the different grassland vegetation types, with the smallest increase for temperate desert steppe (2.2 gC/m2/year) and the largest increase for temperate meadow (5.4 gC/m2/year). The correlation results showed that increased annual precipitation had a positive relationship with the NPP of temperate grasslands. Increased summer and autumn precipitation could increase grassland NPP, particularly for the temperate meadow. With regard to the effects of temperatures, increased temperature, particularly the summer maximum temperature, could decrease annual NPP. However, increased spring minimum temperature could increase the NPP of temperate desert steppe. In addition, this study found, for the first time, an asymmetric relationship between summer nighttime and daytime warming and the NPP of temperate meadow. Specifically, nighttime warming can increase NPP, while daytime warming can reduce NPP in temperate meadow. Our results highlight the importance of including seasonal climate conditions in assessing the vegetation productivity for different grassland types of temperate grasslands and predicting the influences of future climate change on temperate grassland ecosystems.
Emotional Experience and Type of Communication in Oncological Children and Their Mothers: Hearing Their Testimonies Through Interviews
Authors: Barrios Paula , Enesco Ileana , Varea Elena
Published: May 2022

The emotional experience and the type of communication about cancer within the family are important factors for successful coping with pediatric oncology. The main purpose is to study mother’s and children’s emotional experiences concerning cancer, whether they communicate openly about the disease, and relationships between the type of communication and the different emotions expressed by the children. Fifty-two cancer patients aged 6–14 years and their mothers were interviewed in separate sessions about the two central themes of the study: emotional experiences and type of communication. Analyses of response categories were performed to subsequently compare the age-groups and the mother–child responses. According to the results, mothers expressed emotions such as fear, sadness, or anxiety, while children report sadness, pain, but also happiness. Significant positive correlations were observed between mothers’ sadness and older children’s sadness, mothers’ anxiety and children’s fear, and mothers’ anxiety and children’s happiness. Regarding communication type, mothers tend to hide information about the disease from younger children and to provide direct information to the older children. Children usually prefer to communicate their concerns to parents; however, children whose mothers convey anxiety are more likely to prefer to communicate with others. These results support the idea that parents should talk honestly with their children, explaining their illness in an age-appropriate way, and encouraging them to share their emotional experiences. Further studies are needed from a developmental perspective to understand the disease management of children and families.
Psychometric Properties of the Mindfulness Inventory for Sport (German Version)
Authors: Wieczorek Alissa , Renner Karl-Heinz , Schrank Florian , Seiler Kirstin , Wagner Matthias
Published: May 2022

Mindfulness-based training programs are highly established in competitive and recreational sports. One of the best-known approaches is the Mindfulness-Acceptance-Commitment Approach (MAC) by Gardner and Moore), which integrates mindfulness aspects of awareness, non-judgmental attitude, and focus. Based on these aspects, Thienot and colleagues developed and validated an English language sport-specific questionnaire, the so-called Mindfulness Inventory for Sport (MIS), for the assessment of mindfulness skills in athletes. The aim of this study is to psychometrically test a German language version of the MIS (MIS-D). To assess the psychometric properties, the MIS-D was examined in an online survey with an integrated test–retest design (n = 228) for reliability (internal consistency; test–retest reliability), validity (factorial; convergent), and measurement invariance (gender; competition type). The present results support the psychometric quality of the German language version of the MIS. Necessary replications should among others focus on checking the measurement invariance for further relevant subgroups.
Higher Perceived Stress as an Independent Predictor for Lower Use of Emotion-Focused Coping Strategies in Hypertensive Individuals
Authors: Torres Laura Aló , Paradela Regina Silva , Martino Luiza Menoni , da Costa Danielle Irigoyen , Irigoyen Maria Claudia
Published: May 2022

Introduction

Individuals with high scores of perceived stress (PS) are more likely to develop arterial hypertension (AH) than those with low levels of stress. In addition to this, AH and stress are both independent risk factors for executive function (EF) impairment and worse quality of life (QoL). Therefore, strategies to control and cope with emotional stress are of paramount importance. However, less is known about the association of PS with EF, QoL, and coping in individuals with hypertension. This study aimed to evaluate the association of PS with EF performance, coping strategies use, and QoL in a sample of hypertensive patients.

Methods

We assessed a group of 45 hypertensive individuals (mean age = 58.42 ± 8.9 years, 71.11% female). The EF evaluation was: Frontal Assessment Battery; Controlled Oral Word Association Test—FAS; Letter-Number Sequencing subtest from the Wechsler Adult Intelligence Scale—Third Edition (WAIS-III); Digit Span subtest from the Wechsler Memory Scale-Revised (WMS-R) and Wisconsin Card Sorting Test. The type and frequency of coping strategies used were measured by the Brief Coping with Experienced Problems Scale (Brief-COPE). The World Health Organization Quality of Life Questionnaire Bref (WHOQOL-bref) was applied to measure QoL. The associations of the PS with EF performance, coping strategies, and QoL were investigated using univariate and multiple linear regression models adjusted for age, sex, education, systolic pressure, and depression symptoms.

Results

In the multivariate analyses, higher PS was an independent predictor for a lower frequency of emotion-focused strategy use (β = −0.23; p = 0.03). However, PS was not significantly related to EF and Qol in this sample. The lower the PS, the greater the use of emotion-focused coping.

Conclusion

Hypertensive individuals with high PS use less frequently positive emotion-focused coping strategies.
How Accurate Is Your Correlation? Different Methods Derive Different Results and Different Interpretations
Authors: Shao Kaiqi , Elahi Shirvan Majid , Alamer Abdullah
Published: May 2022

Assessing the association between conceptual constructs are at the heart of quantitative research in educational and psychological research. Researchers apply different methods to the data to obtain results about the correlation between a set of variables. However, the question remains, how accurate are the results of the correlation obtained from these methods? Although various considerations should be taken to ensure accurate results, we focus on the types of analysis researchers apply to the data and discuss three methods most researchers use to obtain results about correlation. Particularly, we show how correlation results in bivariate correlation, confirmatory factor analysis (CFA), and exploratory structural equation modeling (ESEM) differ substantially in size. We observe that methods that assume independence of the items often generate inflated factor correlations whereas methods that relax this assumption present uninflated, thus more accurate correlations. Because factor correlations are inflated in bivariate correlation and CFA, the discriminant validity of the constructs is often unattainable. In these methods, the size of the correlation can be very large and biased. We discuss the reasons for this variation and suggest the type of correlation that researchers should select and report.
Brain Derived Neurotrophic Factor and Cognitive Dysfunction in the Schizophrenia-Bipolar Spectrum: A Systematic Review and Meta-Analysis
Authors: Dombi Zsófia B. , Szendi István , Burnet Philip W. J.
Published: May 2022

Background

Cognitive impairment is a core feature of disorders on the schizophrenia-bipolar spectrum, i.e., schizophrenia, bipolar disorder, and schizoaffective disorder. Brain-derived neurotrophic factor (BDNF) has been proposed to be a biomarker of cognitive impairment in these disorders as it plays a critical role in neuroplasticity and proposed to mediate some of the psychotropic effects of medication. However, despite numerous studies investigating the association between circulating BDNF and these disorders, no solid conclusions have been drawn regarding its involvement in cognitive impairment.

Objectives

The current systematic review and meta-analysis aims to examine blood BDNF levels and cognitive dysfunction in patients on the schizophrenia-bipolar spectrum as well as to evaluate whether circulating BDNF measurements can act as a biomarker for cognitive dysfunction.

Methods

Studies were identified by searching Embase and Medline databases for English language articles published in peer-reviewed journals between 2000 January and 2021 June according to the PRISMA guidelines. A total of 815 articles were identified of which 32 met the inclusion criteria for the systematic review – reporting on comparisons between blood BDNF levels and cognitive functions of schizophrenia or bipolar disorder patients versus healthy controls (no studies involving schizoaffective patients were specifically obtained for the time being). Twenty-four of these studies (19 with schizophrenia and 5 with bipolar disorder patients) were eligible to be included in the meta-analysis.

Results

Our findings indicated that circulating BDNF levels were significantly reduced in patients experiencing an acute episode of schizophrenia or bipolar disorder compared to healthy controls. Cognitive function was also found to be significantly worse in patients, however, correlations between BDNF levels and cognitive impairment were not always detected. Interventions, especially pharmacotherapy seemed to improve certain aspects of cognition and increase circulating BDNF levels.

Conclusion

Circulating BDNF alone does not seem to be a valid biomarker of cognitive dysfunction in patients with disorders on the schizophrenia-bipolar spectrum, owing to several confounding factors. Changes of the circulating levels of BDNF should be evaluated in a wider context of other stress-, immune-, and inflammatory-related factors.
Coping Using Sex, Health-Related Behaviors, and Mental Health During COVID-19 Lockdown in the UK
Authors: Daly Natasha , Jones Andrew , Garofalo Carlo , Uzieblo Kasia , Robinson Eric , Gillespie Steven M.
Published: May 2022

Background

Peoples' sexual behaviors have changed during the period of enforced COVID-19 social distancing, in some cases, to cope with negative feelings during lockdown. Research on coping using sex is relatively restricted to samples of men with a history of sexual offending, and it is unknown whether coping using sex is associated with health-related behaviors and mental health in the general population.

Aim

We examined if coping using sex before and during lockdown was associated with adverse outcomes (i.e., self-perceived reduction in health-related behaviors and mental health) in a community sample.

Hypotheses

We hypothesized that participants who reported greater use of sex to cope in the weeks preceding lockdown would show a greater decline in health-related behaviors and mental health during lockdown. Furthermore, that changes in coping using sex resulting from lockdown would account for further variance in the worsening of health-related behaviors and mental health.

Methods

Participants were UK residents, aged 18–60 years, and fluent in English. 789 participants completed an online survey, providing demographic information, self-reported social distancing, loneliness, and coping using sex over a 14-day period during lockdown, and retrospectively preceding lockdown.

Outcomes

Participants reported perceived changes in health-related behaviors and mental health symptomatology during lockdown compared to before the pandemic. They also self-reported levels of stress, anxiety and depression during lockdown.

Results

Greater coping using sex prior to lockdown predicted positive change in health-related behaviors, for example, higher scores were associated with participants reporting having exercised and slept more. It was also associated with higher trait levels of anxiety, stress and depression during lockdown. Changes in coping using sex from before to during lockdown did not predict perceived changes in health related behaviors or mental health symptomatology.

Conclusions

Overall, greater coping using sex prior to lockdown was associated with worse mental health symptomatology during lockdown (anxiety, depression and stress), however, it was also associated with perceived positive change in health-related behaviors compared with before lockdown. This suggests that coping using sex may be associated with negative emotional reactions during lockdown, but may also be linked with positive change in health-promoting behaviors.
The Impact of COVID-19 Restrictions on the Healthy Eating and Movement Behaviors of 0–12-Year-Old Children in Western Sydney, Australia
Authors: McNicholas Janelle , Hammersley Megan L. , Hopkins Stacey , McDermott Sarah , Plaskett Jennifer
Published: May 2022

This study examined effects of COVID-19 restrictions in early 2020 on movement (physical activity, sedentary behavior and sleep) and healthy eating behaviors in families with 0–12-year-old children in western Sydney, Australia. A total of 1,371 parents completed an online survey about changes in children's and families' food intake and movement behaviors. There was an increase in sedentary screen use by children (4.18/5.00) and families (3.91/5.00) and a slight increase in reported physical activity (3.56/5.00), amount of food consumed (3.58/5.00) and meals and snacks eaten (3.69/5.00) during the height of the COVID-19 restrictions compared to before. There was little change in reported sleep (3.17/5.00). Lower socioeconomic families were disproportionately affected, with greater increases in unhealthy eating (t = 2.739, P = 0.06), lower levels of improvement in physical activity, such as walking and cycling (t = −7.521, P < 0.001) and outdoor activity (t = 5.415, p < 0.001), and higher increases in family sedentary behavior (t = 2.313, P = 0.021). Therefore, even short periods of restrictions can result in detrimental health behavior changes. Such changes could become entrenched leading to increased risk of lifestyle diseases. Programmatic and policy strategies should be geared toward promoting healthy movement behaviors, focusing on families of lower socioeconomic status to ensure the pandemic does not widen an existing gap.
Vaccine Hesitancy Drives Low Human Papillomavirus Vaccination Coverage in Girls Attending Public Schools in South Africa
Authors: Khosa Languta A. , Meyer Johanna C. , Motshwane Feni M. M. , Dochez Carine , Burnett Rosemary J.
Published: May 2022

Girls aged ≥9 years attending South African public sector schools are provided with free human papillomavirus (HPV) vaccination, through a schools-based programme. HPV vaccine misinformation spread via social media in 2014, was identified as a barrier to obtaining parental informed consent in some districts, including Sedibeng District, which subsequently had the lowest HPV vaccination coverage in Gauteng Province in 2018. This study investigated vaccine hesitancy in caregivers of girls in Grade 4 to 7 aged ≥9 years attending public schools in Sedibeng District. A cross-sectional survey using a self-administered questionnaire was conducted among caregivers of age-eligible girls attending all public schools in Sedibeng District with first dose HPV vaccination coverage of <70%. The questionnaire included demographics; HPV vaccination status of girls; reasons for not being vaccinated; and a 5-item tool measuring the determinants of vaccine hesitancy (5C scale), using a 7-point Likert scale. Data were coded and captured on Microsoft Excel®. Except for collective responsibility which was reverse scored, the other 5C items (confidence, complacency, constraints, and calculation) were captured as follows: 1 = strongly disagree, 2 = moderately disagree, 3 = slightly disagree, 4 = neutral, 5 = slightly agree, 6 = moderately agree and 7 = strongly agree. Descriptive and inferential statistical analyses were conducted using Epi InfoTM. Of the principals of all schools with <70% HPV vaccination coverage, 69.6% (32/46) gave permission. The response rate from caregivers of girls present on the day of data collection was 36.8% (1,782/4,838), with 67.1% (1,196/1,782) of respondents reporting that their daughters had received ≥1 dose of HPV vaccine. Only 63.1% (370/586) of respondents with unvaccinated daughters answered the question on reasons, with 49.2% (182/370) reporting reasons related to vaccine hesitancy. Statistically significant differences between caregivers of vaccinated and unvaccinated daughters were identified for four of the five determinants of vaccine hesitancy: confidence (vaccinated group higher), complacency (unvaccinated group higher), constraints (unvaccinated group higher) and collective responsibility (vaccinated group higher). This is the first South African study to (a) report results of the 5C scale, which was found to be very useful for predicting vaccination uptake; and (b) confirm that the relatively low HPV vaccination coverage in Sedibeng District is largely driven by reasons related to vaccine hesitancy.
Delayed Diagnosis and Treatment of Cancer Patients During the COVID-19 Pandemic in Henan, China: An Interrupted Time Series Analysis
Authors: Liu Changpeng , Piao Heng , Zhang Tao , Yang Dongjian , Li Xiaoyan , Tang Xiance
Published: May 2022

Objective

To investigate the possible impact of lockdown policies on the diagnosis and treatment of cancer patients in Henan, China.

Design, Setting, and Participants

We collected data from the Henan Cancer Hospital, affiliated with Zhengzhou University. The monthly numbers of inpatient admissions from January 2014 to December 2019 were used to forecast the number of inpatient admissions in 2020, which was then compared to the actual number of patients admitted during the pandemic to evaluate how the actual number diverges from this forecast. We conducted an interrupted time series analysis using the autoregressive integrated moving average (ARIMA) model.

Main Outcomes and Measures

For specific diagnoses, treatment modalities, and age groups, we compared the changes in monthly admissions after the pandemic with the forecasted changes from the model.

Results

The observed overall monthly number of inpatient admissions decreased by 20.2% [95% confidence interval (CI), 11.7–27.2%], 78.9% (95% CI, 77.3–80.4%), and 40.9% (95% CI, 35.6–45.5%) in January, February, and March 2020, respectively, as compared with those predicted using the ARIMA model. After the lockdown, visits for all treatment modalities decreased sharply. However, apparent compensation and recovery of the backlog appeared in later surgeries. As a result, the number of patients who underwent surgery in 2020 (30,478) was close to the number forecasted by the ARIMA model (30,185). In the same period, patients who received other treatments or underwent examinations were 106,074 and 36,968, respectively; the respective numbers that were forecasted by ARIMA were 127,775 and 60,025, respectively. These findings depict a decrease of 16.9 and 38.4% in patients who received other treatments or underwent examinations only, respectively. Regarding diagnosis, the reported incidence of various cancers decreased dramatically in February, with varying extent and speed of recovery.

Conclusion and Relevance

The COVID-19 pandemic has significantly delayed the diagnosis and treatment of cancer in Henan, China. Long-term research should be conducted to assess the future effects of lockdown policies.
Automatic Calibration of the Adaptive 3D Scanner-Based Robot Welding System
Authors: Arko Peter , Jezeršek Matija
Published: May 2022

An advanced automatic calibration procedure and its versatile usage in the context of the adaptive robot welding technology are presented. The 3D scanner-based robot welding system calibration is composed of the measurement of the reference plate and numerical optimization of the hand-eye and intrinsic parameters by minimizing the deviation between the measured and reference plate. The measurements of the reference plate are acquired from various robot poses (typically 15). The shape features of the reference plate are then detected, and finally, the calculation of hand-eye and intrinsic parameters is performed using Powell’s optimization algorithm, where the merit function presents an average deviation between the measured and reference geometry. Validation experiments show appropriate system accuracy which is better than 0.06 mm perpendicular to the scanning direction. This calibration procedure’s important features are complete automation and fast execution times (approximately 90 s). This enables its implementation into a regular daily robot self-maintenance and monitoring plan. The universal use of such a robot welding system is demonstrated in multi-layer heavy-duty welding of thick pipes on cast machined hollow parts and in precise laser welding of thin sheet metal parts.
Can a Guided Zoo Tour Improve Connection to Nature?
Authors: Kleespies Matthias W. , Dierkes Paul W.
Published: May 2022

Humans’ connections to nature have been declining over time. This is problematic because people with a weaker connections to nature pay less attention to the environment and tend to show less environmentally friendly behavior. Environmental education programs are one way to address our declining connection to nature. In our study, we examined whether a 1-h guided zoo tour could improve high school students’ connections to nature. We also examined the impact of five add-ons to the tour (feeding giraffes or meerkats, a behind-the-scenes look, a keeper talk, and a visit to the petting zoo). We found that even a short environmental education program can increase students’ connections to nature. Special experiences, such as feeding a giraffe, showed a particularly positive effect, while negative experiences, such as disgust, led to a slightly negative effect. Our study provides evidence for the effectiveness of short environmental education programs in reconnecting people to nature.
Prevalence of Prohibited Substance Use and Methods by Female Athletes: Evidence of Gender-Related Differences
Authors: Collomp Katia , Ericsson Magnus , Bernier Nathan , Buisson Corinne
Published: May 2022

To achieve optimal sports performances, women and men may show specific doping practices because of the physiological and psychological gender differences, but there are few data on this topic. Here, we report the apparent use of prohibited substances and methods by female athletes based on analyses of the doping tests collected by the French Anti-Doping Agency from 2013 to 2019. We compared the frequency of use and the ergogenic and side effects to those of their male counterparts. The results revealed lower use of prohibited substances in female vs. male athletes, with significantly fewer anabolic agents, hormone and metabolic modulators, and cannabinoids. Gender specificity in utilization of substance classes was also shown. Relatively lower use of hormone modulators and cannabinoids and higher use of beta-2 agonists, diuretics and glucocorticoids were found in the woman cohort compared with men cohort, combined with the different choice of substances, possibly because of the altered ergogenic and/or side effects. However, no impact due to gender regarding the sports disciplines was observed, with both women and men showing similar use of anabolic agents, mainly in the anaerobic sports, and EPO and corticoids, mainly in endurance or mixed sports. Further studies are needed to put these French data into a global perspective, comparing uses across countries and exploring possible new developments in the fight against doping in women.
Comparison of Shoulder Kinematics and Muscle Activation of Female Elite Handball Players With and Without Pain—An Explorative Cross-Sectional Study
Authors: Torabi Tina Piil , Juul-Kristensen Birgit , Dam Mogens , Zebis Mette K. , van den Tillaar Roland , Bencke Jesper
Published: May 2022

Non-traumatic shoulder injuries are common in team handball. However, many athletes continue to throw, despite pain in the shoulder. This study investigated upper body kinematics and muscle activation while throwing in female elite handball players with and without shoulder pain. Thirty female elite team handball players, 15 with pain (age 22.2 ± 2.9 yrs.) and 15 without pain (age 20.4 ± 2.6 yrs.) performed five standing throws in which joint kinematics and muscle activity were measured in the following muscles: pectoralis major, infraspinatus, serratus anterior, latissimus dorsi, and upper-, middle-, and lower trapezius. The main findings revealed that peak joint angles and angular velocities were not different between groups; however, group differences were observed in earlier timing of position and longer time spent in maximal shoulder extension and external shoulder rotation in the pain group compared with the no pain group. The pain group also revealed a significant lower muscle peak activity in the serratus anterior during the cocking phase compared to the no pain group. After the cocking phase and at ball release, the groups had similar activation. In conclusion, the present study showed group differences in appearance and time spent in maximal humerus extension and external rotation and a different serratus anterior muscle peak activity between elite handball players playing with and without shoulder pain, which are identified as possible mechanisms of adaptation to avoid pain.
Effect of Maternal HIV Infection on Infant Development and Outcomes
Authors: Ruck Candice E. , Smolen Kinga K.
Published: May 2022

Early life represents a period of profound immunological development and heightened susceptibility to infectious disease. The developmental trajectory over this period is influenced by a number of factors, including gestational age, mode of delivery, mode of feeding, microbiome development, and environmental exposures. There are also several maternal factors that have been shown to have a negative effect on both immune development and clinical outcomes, including maternal infection and inflammation. Studies have associated maternal HIV infections with an increase in infectious morbidity and mortality and decreased growth measures among their HIV-exposed uninfected (HEU) offspring. Among HEU infants, socioeconomic factors, maternal nutrition, maternal viral load, and maternal inflammation have also all been associated with impaired infant immune status and clinical outcomes. However, the mechanisms underlying these observations have not been elucidated and, apart from measures of disease severity, few studies thus far have undertaken in-depth assessments of maternal health status or immune function during gestation and how these influence developmental outcomes in their infants. The lack of a mechanistic understanding of how these gestational influences affect infant outcomes inhibits the ability to design and implement effective interventions. This review describes the current state of research into these mechanisms and highlights areas for future study include; how HIV infection causes the inflammatory trajectory to deviate from normal gestation, the mechanism(s) by which in utero exposure to maternal inflammation influences infant immune development and clinical outcomes, the role of socioeconomic factors as an inducer of maternal stress and inflammation, and maternal nutrition during gestation.
Immune Responses of a Novel Bi-Cistronic SARS-CoV-2 DNA Vaccine Following Intradermal Immunization With Suction Delivery
Authors: Jeong Moonsup , Kudchodkar Sagar B. , Gil Areum , Jeon Bohyun , Park Gee Ho , Cho Youngran , Lee Hyojin , Cheong Mi Sun , Kim Wonil , Hwang Yun-Ho , Lee Jung-Ah , Lim Heeji , Kim Mi Young , Lallow Emran O. , Brahmbhatt Tej , Kania Stephen A. , Jhumur Nandita C. , Shan Jerry W. , Zahn Jeffrey D. , Shreiber David I. , Singer Jonathan P. , Lin Hao , Spiegel Erin K. , Pessaint Laurent , Porto Maciel , Van Ry Alex , Nase Danielle , Kar Swagata , Andersen Hanne , Tietjen Ian , Cassel Joel , Salvino Joseph M. , Montaner Luis J. , Park Young K. , Muthumani Kar , Roberts Christine C. , Maslow Joel N.
Published: May 2022

SARS-CoV-2 is the third pathogenic coronavirus to emerge since 2000. Experience from prior outbreaks of SARS-CoV and MERS-CoV has demonstrated the importance of both humoral and cellular immunity to clinical outcome, precepts that have been recapitulated for SARS-CoV-2. Despite the unprecedented rapid development and deployment of vaccines against SARS-CoV-2, more vaccines are needed to meet global demand and to guard against immune evasion by newly emerging SARS-CoV-2 variants. Here we describe the development of pGO-1002, a novel bi-cistronic synthetic DNA vaccine that encodes consensus sequences of two SARS-CoV-2 antigens, Spike and ORF3a. Mice immunized with pGO-1002 developed humoral and cellular responses to both antigens, including antibodies and capable of neutralizing infection by a clinical SARS-CoV-2 isolate. Rats immunized with pGO-1002 by intradermal (ID) injection followed by application of suction with our GeneDerm device also developed humoral responses that included neutralizing antibodies and RBD-ACE2 blocking antibodies as well as robust cellular responses to both antigens. Significantly, in a Syrian hamster vaccination and challenge model, ID+GeneDerm-assisted vaccination prevented viral replication in the lungs and significantly reduced viral replication in the nares of hamsters challenged with either an ancestral SARS-CoV-2 strain or the B.1.351 (Beta) variant of concern. Furthermore, vaccinated immune sera inhibited virus-mediated cytopathic effects in vitro. These data establish the immunogenicity of the SARS-CoV-2 vaccine candidate pGO-1002 which induces potent humoral and cellular responses to the Spike and ORF3a antigens and may provide greater protection against emerging variants.
Construction of a Metabolism-Related Long Non-Coding RNAs-Based Risk Score Model of Hepatocellular Carcinoma for Prognosis and Personalized Treatment Prediction
Authors: Zhang Peichen , Chen Liping , Wu Shengjie , Ye Bailiang , Chen Chao , Shi Lingyan
Published: May 2022

Background: Long non-coding RNAs (lncRNAs) play a key regulatory role in tumor metabolism. Although hepatocellular carcinoma (HCC) is a metabolic disease, there have been few systematic reports on the association between lncRNA expression and metabolism in HCC.

Results: In this study, we screened 557 metabolism-related lncRNAs in HCC. A risk score model based on 13 metabolism-related lncRNA pairs was constructed to predict the outcome and drug response in HCC. The risk score model presented a better prediction of the outcomes than that with common clinicopathological characteristics, such as tumor stage, grade, and status and aneuploidy score in both training and testing cohorts. In addition, patients in the high-risk group exhibited higher responses to gemcitabine and epothilone, whereas those in the low-risk group were more sensitive to metformin and nilotinib.

Conclusion: The metabolism-related lncRNAs-based risk score model and the other findings of this study may be helpful for HCC prognosis and personalized treatment prediction.
Structural Model of Straw Briquetting Machine with Vertical Ring Die and Optimization of Briquetting Performance
Authors: Chen Shuren , Zhao Yunfei , Tang Zhong , Ding Hantao , Su Zhan , Ding Zhao
Published: May 2022

The solidification and molding of straw has been an effective method for comprehensive utilization of straw resources. However, the existing die-roll extrusion-type straw briquetting machine has challenges, such as the easy blockage of ring die holes and the unstable quality of the briquette. In this paper, the influence of four factors, including moisture content of straw, molding temperature, clearance between die and roller, and spindle speed on the quality of the briquette were studied. The regression model of the relaxed density and impact resistance of the briquette were established to obtain the optimal values of these factors to provide the best parameters for producing straw briquette. The results indicate that under the experimental conditions of moisture content 22.335%, temperature 85.127 °C, clearance between die and roller 3.099 mm, and spindle speed 172.712 r/min, the maximum relaxed density and impact resistance of the briquette were 1.144 g/cm3 and 74.76%, respectively. The performance of the briquette already meets the requirements for combustion, transportation, and storage.
The Impact of the Renovation of Grassland on the Development of Segetal Weeds in Organic Farming
Authors: Gaweł Eliza , Grzelak Mieczysław , Waliszewska Bogusława , Janyszek-Sołtysiak Magdalena
Published: May 2022

The intensive use or discontinuation of the use of swards can compromise biodiversity, yields, and feed quality; thus, leading to the degradation of permanent grasslands. Various methods of renovation are employed to restore the usability of degraded swards. In the years 2013–2016, a monofactorial field experiment was carried out on the Experimental Farm in Grabów (province of Mazowieckie, Poland). The experiment involved swards being reseeded after ploughing (P) and after disking with a compact harrow (H), with a non-renovated sward as the control treatment. The plots under renovation were reseeded with a lucerne–grass mixture. Both seedbed preparation methods for the renewed sward, ploughing (P) or shallow disking to a depth of 5 cm (H), were found to be effective for increasing sward yields, restricting weed growth, and reducing the number of weeds in the sward. The ploughing-based renovation method (P) had a strongly restrictive effect on biodiversity, as expressed by the H’ function value vis-à-vis the harrow method (H) and the non-renewed control (NR). Dicotyledonous species accounted for 92.3% of the weed population in the studied treatments. Of these, Taraxacum officinale, Achillea milefolium, Capsella bursa-pastoris, and Plantago maior occurred in the highest numbers.
Response of Maize, Cotton, and Soybean to Increased Crop Density in Heterogeneous Planting Arrangements
Authors: Ethridge Sandra R. , Locke Anna M. , Everman Wesley J. , Jordan David L. , Leon Ramon G.
Published: May 2022

The reduction of row spacing and increase of crop population density are important tools for maximizing crop yield. For this strategy to be effective, the crop population should not create intraspecific crop competition that penalizes yield. Thus, planting arrangements that increase light interception throughout the canopy without increasing row spacing might be needed to maintain yield. In this study, heterogeneous planting arrangements on evenly spaced rows were analyzed for maize (Zea mays L.), cotton (Gossypium hirsutum L.), and soybean (Glycine max (L.) Merr.). Each crop had four planting arrangements: (1) normal density in all rows, considered the control, (2) doubled density in all rows, (3) a sequential arrangement of normal and tripled densities (each in every other row; NTNT), and (4) normal-tripled-tripled-normal (NTTN). Maize and cotton did not exhibit changes in growth and architecture when comparing uniform and variable planting arrangements. Soybeans were more adaptable and increased biomass production by 44% to 45% in variable arrangements. None of the crops showed differences in yield due to planting arrangement, so the use of rows with different densities might not be needed when using high densities to maximize yield.
Effects of Soil Water Regulation on the Cotton Yield, Fiber Quality and Soil Salt Accumulation under Mulched Drip Irrigation in Southern Xinjiang, China
Authors: He Pingru , Yu Shuang’en , Zhang Fucang , Ma Tao , Ding Jihui , Chen Kaiwen , Chen Xin , Dai Yan
Published: May 2022

To optimize suitable water-saving and soil salt-controlling irrigation needed for the high yield and good quality of cotton in southern Xinjiang, a field experiment was carried out to study the effects of soil water lower limits on water consumption, water use efficiency (WUE), yield, cotton fiber quality and soil salt accumulation under mulched drip irrigation in Korla, Xinjiang. The field capacity (FC) was regarded as the upper limit of soil moisture, and five soil water lower limits (85% FC, 75% FC, 65% FC, 55% FC, 45% FC, referred as T1~T5, respectively) were designed during the cotton growth period. The results indicated that the irrigation frequency and irrigation quota of cotton were gradually increased with the increase in the soil water lower limit, while the water consumption modulus for T2 treatment during the critical period of water demand arrived at the maximum value. Moreover, with the decrease in the soil water lower limit, the WUE, fiber micronaire value and fiber maturity index of cotton increased, whereas the yield, nitrogen partial factor productivity (PFPN) and fiber breaking elongation of cotton decreased. However, when the soil water lower limit exceeded 75% FC, the increase had little effect on the cotton yield increase and PFPN improvement, and the yield and PFPN for T2 treatment were 7146.4 kg∙hm−2 and 23.82 kg∙kg−1, respectively, In addition, the decrease in the soil water lower limit was unfavorable for an increase in fiber length, but it was conducive to the enhancement of fiber strength. Furthermore, soil salt accumulated inside and outside the film for the designed soil water lower limits, and the amount of accumulated salt in 0~100 cm followed T3 > T5 > T1 > T2 > T4. Based on a comprehensive analysis with the entropy TOPSIS method, the findings of the present study suggested that the suitable soil water lower limit for cotton under mulched drip irrigation was 75% FC in southern Xinjiang, China.
Clinical Efficacy and Safety of Vancomycin Continuous Infusion in Patients Treated at Home in an Outpatient Parenteral Antimicrobial Therapy Program
Authors: Thijs Lore , Quintens Charlotte , Vander Elst Lotte , De Munter Paul , Depypere Melissa , Metsemakers Willem-Jan , Vles Georges , Liesenborghs Astrid , Neefs Jens , Peetermans Willy E. , Spriet Isabel
Published: May 2022

Vancomycin is commonly used in outpatient parenteral antimicrobial therapy (OPAT) of Gram-positive infections. Therapeutic drug monitoring and adverse event monitoring pose a challenge. Outcome data of vancomycin in OPAT (vOPAT) are limited. The study aim was to report the safety and efficacy of a structured vOPAT program implemented in the University Hospitals Leuven. The program provides continuous elastomeric infusion of vancomycin at home with biweekly follow-up at the outpatient clinic. Demographics, clinical, biochemical and treatment parameters, target attainment parameters and clinical outcomes were recorded. An e-survey was conducted to assess patient satisfaction. Thirty-five vOPAT episodes in 32 patients were included. During 206 follow-up consultations, 203 plasma concentration measurements were registered with a median vancomycin plasma concentration of 22.5 mg/L (range 6.6–32.0). The majority of concentrations (68.5%) were within the therapeutic range (20.0–25.0 mg/L). Adverse event rates, including drug- (5.7%) and catheter-related (5.7%) events, were low. For 32 vOPAT episodes, a clinical cure rate of 100% was observed. All patients who completed the e-survey were satisfied with their vOPAT course. These findings show that a structured vOPAT program with rigorous follow-up provides safe and effective ambulatory treatment of patients with vancomycin in continuous infusion.