bluuebunny/crossref_metadata_2025 huggingface dataset:
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
Authors: Webber Beau R. , Lonetree Cara-lin , Kluesner Mitchell G. , Johnson Matthew J. , Pomeroy Emily J. , Diers Miechaleen D. , Lahr Walker S. , Draper Garrett M. , Slipek Nicholas J. , Smeester Branden A. , Lovendahl Klaus N. , McElroy Amber N. , Gordon Wendy R. , Osborn Mark J. , Moriarity Branden S.
Published: November 2019

The fusion of genome engineering and adoptive cellular therapy holds immense promise for the treatment of genetic disease and cancer. Multiplex genome engineering using targeted nucleases can be used to increase the efficacy and broaden the application of such therapies but carries safety risks associated with unintended genomic alterations and genotoxicity. Here, we apply base editor technology for multiplex gene modification in primary human T cells in support of an allogeneic CAR-T platform and demonstrate that base editor can mediate highly efficient multiplex gene disruption with minimal double-strand break induction. Importantly, multiplex base edited T cells exhibit improved expansion and lack double strand break-induced translocations observed in T cells edited with Cas9 nuclease. Our findings highlight base editor as a powerful platform for genetic modification of therapeutically relevant primary cell types.
A LONELY GUY protein of Bordetella pertussis with unique features is related to oxidative stress
Authors: Moramarco Filippo , Pezzicoli Alfredo , Salvini Laura , Leuzzi Rosanna , Pansegrau Werner , Balducci Enrico
Published: November 2019

The Gram-negative bacterium B. pertussis is the causative agent of whooping cough. This infection is re-emerging and new features related to Bordetella pathogenesis and microbiology could be relevant to defeat it. Therefore, we focused our attention on BP1253, a predicted exported protein from B. pertussis erroneously classified as lysine decarboxylase. We showed that BP1253 shares the highly conserved motif PGGxGTxxE and the key catalytic amino-acid residues with newly structurally characterized “LONELY GUY” (LOG) proteins. Biochemical studies have confirmed that this protein functions as a cytokinin-activating enzyme since it cleaves the N-glycosidic linkage between the base and the ribose, leading to the formation of free bases, which are the active form of plant hormones called cytokinins. Remarkably, BP1253 selectively binds monophosphate nucleotides such as AMP, GMP and CMP, showing a wider variety in binding capacity compared to other LOGs. Cytokinin production studies performed with B. pertussis have revealed 6-O-methylguanine to be the physiological product of BP1253 in agreement with the higher activity of the enzyme towards GMP. 6-O-methylguanine is likely to be responsible for the increased sensitivity of B. pertussis to oxidative stress. Although BP1253 has a primary sequence resembling the hexameric type-II LOGs, the dimeric state and the presence of specific amino-acids suggests that BP1253 can be classified as a novel type-II LOG. The discovery of a LOG along with its product 6-O-methylguanine in the human pathogen B. pertussis may lead to the discovery of unexplored functions of LOGs, broadening their role beyond plants.
DDR1 autophosphorylation is a result of aggregation into dense clusters
Authors: Corcoran David S. , Juskaite Victoria , Xu Yuewei , Görlitz Frederik , Alexandrov Yuriy , Dunsby Christopher , French Paul M. W. , Leitinger Birgit
Published: November 2019

The collagen receptor DDR1 is a receptor tyrosine kinase that promotes progression of a wide range of human disorders. Little is known about how ligand binding triggers DDR1 kinase activity. We previously reported that collagen induces DDR1 activation through lateral dimer association and phosphorylation between dimers, a process that requires specific transmembrane association. Here we demonstrate ligand-induced DDR1 clustering by widefield and super-resolution imaging and provide evidence for a mechanism whereby DDR1 kinase activity is determined by its molecular density. Ligand binding resulted in initial DDR1 reorganisation into morphologically distinct clusters with unphosphorylated DDR1. Further compaction over time led to clusters with highly aggregated and phosphorylated DDR1. Ligand-induced DDR1 clustering was abolished by transmembrane mutations but did not require kinase activity. Our results significantly advance our understanding of the molecular events underpinning ligand-induced DDR1 kinase activity and provide an explanation for the unusually slow DDR1 activation kinetics.
Xiphosurid from the Tournaisian (Carboniferous) of Scotland confirms deep origin of Limuloidea
Authors: Bicknell Russell D. C. , Pates Stephen
Published: November 2019

Horseshoe crabs are archetypal marine chelicerates with a fossil record extending from the Lower Ordovician to today. The major horseshoe crab groups are thought to have arisen in the middle to late Palaeozoic. Here we present the oldest known limuloid from the lower Carboniferous (Tournaisian stage, c. 350 million years ago) of Scotland: Albalimulus bottoni gen. et sp. nov. A comprehensive phylogenetic analysis supports the placement of A. bottoni as a representative of the extant family Limulidae and 100 million years older than any other limulid taxon. The use of geometric morphometric analyses corroborate the erection of the new taxon and illustrates the exploitation of morphospace by xiphosurids. This new taxon highlights the complex evolutionary history of xiphosurids and the importance of documenting these unique Palaeozoic individuals.
Water hydrogen uptake in biomolecules detected via nuclear magnetic phosphorescence
Authors: Sadet Aude , Stavarache Cristina , Teleanu Florin , Vasos Paul R.
Published: November 2019

We introduce a new symmetry-based method for structural investigations of areas surrounding water-exchanging hydrogens in biomolecules by liquid-state nuclear magnetic resonance spectroscopy. Native structures of peptides and proteins can be solved by NMR with fair resolution, with the notable exception of labile hydrogen sites. The reason why biomolecular structures often remain elusive around exchangeable protons is that the dynamics of their exchange with the solvent hampers the observation of their signals. The new spectroscopic method we report allows to locate water-originating hydrogens in peptides and proteins via their effect on nuclear magnetic transitions similar to electronic phosphorescence, long-lived coherences. The sign of long-lived coherences excited in coupled protons can be switched by the experimenter. The different effect of water-exchanging hydrogens on long-lived coherences with opposed signs allows to pinpoint the position of these labile hydrogen atoms in the molecular framework of peptides and proteins.
High-fat diet impairs cognitive function of zebrafish
Authors: Meguro Shinichi , Hosoi Sayaka , Hasumura Takahiro
Published: November 2019

An unhealthy diet with excessive fat intake has often been claimed to induce not only obesity but also cognitive dysfunction in mammals; however, it is not known whether this is the case in zebrafish. Here, we investigated the effect of excessive fat in the diet on cognitive function and on gene expression in the telencephalon of zebrafish. Cognitive function, as measured by active avoidance test, was impaired by feeding of a high-fat diet compared with a control diet. In RNA sequencing analysis of the telencephalon, 97 genes were identified with a fold change in expression greater than 2 and a p-value less than 0.05 between the two diets. In quantitative real-time PCR analysis of the telencephalon, genes related to neuronal activity, anti-oxidative stress, blood–brain barrier function and amyloid-β degradation were found to be downregulated, whereas genes related to apoptosis and amyloid-β production were found to be upregulated, in the high-fat diet group, which are changes known to occur in mammals fed a high-fat diet. Collectively, these results are similar to those found in mammals, suggesting that zebrafish can serve as a suitable animal model in research into cognitive impairment induced by excessive fat in the diet.
Publisher Correction: Development of an efficient cytosolic isobutanol production pathway in Saccharomyces cerevisiae by optimizing copy numbers and expression of the pathway genes based on the toxic effect of α-acetolactate
Authors: Park Seong-Hee , Hahn Ji-Sook
Published: November 2019

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Crystallization of zinc oxide quantum dots on graphene sheets as an anode material for lithium ion batteries
Authors: Tan Qingke , Kong Xiangli , Guan Xianggang , Wang Chao , Xu Binghui
Published: November 2019

A zinc oxide quantum dot/reduced graphene oxide (ZnO/RGO) composite is prepared for the first time by a stepped graphene oxide (GO) reduction strategy.
Exploring the semiconductor properties of a charge transfer cocrystal of 1-aminopyrene and TCNQ
Authors: Mandal Arkalekha , Choudhury Anwesha , Kumar Rahul , Iyer Parameswar Krishnan , Mal Prasenjit
Published: November 2019

The n-type semiconductor nature of a 1 : 1 mixed stack charge transfer cocrystal of 1-aminopyrene and TCNQ is explored.
Synthesis of MIL-53 thin films by vapour-assisted conversion
Authors: Warfsmann Jan , Tokay Begum , Champness Neil R.
Published: November 2019

Using a vapour-assisted conversion approach it is possible to prepare homogeneous MIL-53 thin films on a variety of substrates.
Emerging strategies for the activity assay and inhibitor screening of alpha-glucosidase
Authors: Zhang Xianlong , Li Guoliang , Wu Di , Yu Yanxin , Hu Na , Wang Honglun , Li Xiuting , Wu Yongning
Published: November 2019

The high incidence of diabetes mellitus has caused widespread concern around the world, and has quickly become one of the most prevalent and costly chronic diseases.
Xanthohumol inhibits tau protein aggregation and protects cells against tau aggregates
Authors: Zhang Mohan , Wu Qiuping , Yao Xuanbao , Zhao Junyi , Zhong Weicong , Liu Qiong , Xiao Shifeng
Published: November 2019

Xanthohumol is shown to interact with tau protein and inhibit its aggregation.
Evaluation of anti-platelet activity of grape pomace extracts
Authors: Choleva Maria , Boulougouri Vassiliki , Panara Anthi , Panagopoulou Eirini , Chiou Antonia , Thomaidis Nikolaos S. , Antonopoulou Smaragdi , Fragopoulou Elizabeth
Published: November 2019

Ethanol–water extracts from red grape pomace could be used for production of functional foods with anti-platelet properties.
Correction: Electrochemical paper-based devices: sensing approaches and progress toward practical applications
Authors: Noviana Eka , McCord Cynthia P. , Clark Kaylee M. , Jang Ilhoon , Henry Charles S.
Published: November 2019

Correction for ‘Electrochemical paper-based devices: sensing approaches and progress toward practical applications’ by Eka Noviana et al., Lab Chip, 2019, DOI: 10.1039/c9lc00903e.
Improved current efficiency of quasi-2D multi-cation perovskite light-emitting diodes: the effect of Cs and K
Authors: Yang Guang , Liu Xiyuan , Sun Yizhe , Teng Chao , Wang Yong , Zhang Shengdong , Zhou Hang
Published: November 2019

Multiple cation-substitution is an effective method to enhance the performance of organic–inorganic hybrid quasi-2D perovskite light-emitting diodes.
Modulated transition metal–oxygen covalency in the octahedral sites of CoFe layered double hydroxides with vanadium doping leading to highly efficient electrocatalysts
Authors: Yang Yibin , Ou Yingqing , Yang Yang , Wei Xijun , Gao Di , Yang Lin , Xiong Yuli , Dong Hongmei , Xiao Peng , Zhang Yunhuai
Published: November 2019

The development of efficient and stable non-noble-metal electrocatalytic materials for the oxygen evolution reaction (OER) is a huge and important challenge at present.
Regeneration scanning method for M‐WFRFT communication signals
Authors: Liu Fang , Feng Yongxin
Published: November 2019

Considering its simplicity and the uniform distribution of signal energy and interference after transformation, the weighted fractional Fourier transform (WFRFT) has been gradually applied to the field of communication. With in‐depth study of WFRFT, the number of weighted terms can be extended from the original four terms to any number. This is called a multi‐WFRFT (M‐WFRFT). As the number of weighted terms plays an important role in the receiving system, research into an M‐WFRFT reception method compatible with different weighted terms is critical. In view of the high complexity of communication systems based on M‐WFRFT, in order to solve the general receiving problems of the receiver when the parameters of the transmitter are not fixed or when multiple transmitters share the same receiving system, a regenerative transformation scan method for M‐WFRFT signals is established. This is accomplished by adapting to dynamic processing conditions and avoiding the problem of high complexity. In this method, the 4‐WFRFT mechanism is introduced, regeneration weighting coefficients are constructed, and the regeneration order is given by combining the inherent relationship between the weighting coefficients and the order. The new method can achieve the purpose of receiving M‐WFRFT signals with different number of items and different orders.
Performance improvement for machine learning‐based cooperative spectrum sensing by feature vector selection
Authors: Wu Wen , Li Zan , Ma Shuai , Shi Jia
Published: November 2019

To explore the potential of machine learning‐based cooperative spectrum sensing (CSS) in training time, classification speed and classification performance, this study mainly focuses on studying the problem of the feature vectors selecting for machine learning‐based CSS. First, a new machine learning‐based CSS framework is presented, in which, energy vector forming module, feature vector conversion module, training module, classification module and training sample database are included. Second, a new two‐dimensional distance vector is developed, and it is converted by an m‐dimensional energy vector according to the distance measurement between vectors. Furthermore, six combination modes are obtained by combining three feature vectors (energy, probability and distance vectors) with two supervised machine learning methods, which are support vector machine (SVM) and weighted K‐nearest‐neighbour, respectively. From the proposed experimental simulations, the authors can find that the distance vector is obviously superior to the probability vector in computation time. Moreover, the probability vector and distance vector are superior to the energy vector in training time except for the case of poor signal and fewer users, and obviously superior to the energy vector in classification speed. At last, the probability vector and distance vector with SVM classifier show the best classification performance in six combination modes.
Absorptive/transmissive integrated frequency selective structure based on lumped resistance elements
Authors: Wang Aiai , Yan Mingbao , Zhang Jieqiu , Zhen Lin , Wang Jiafu , Qu Shaobo
Published: November 2019

In this study, an absorptive/transmissive integrated frequency selective surface based on lumped resistance elements is presented. This technique is based on loading the lumped resistance element on the square ring, cascading a special filter. A flat band response can be obtained from the two transmission poles in each transmission window. Simulated results show that the −0.5 dB bandwidths are about 1.61 GHz (3.63–5.24 GHz) and 3.35 GHz (14.22–17.56 GHz), respectively. There is a wide absorptive band between the two transmission windows. The −10 dB absorptive bandwidth is about 5.16 GHz (8.04–13.20 GHz). In addition, the design procedure and operating principle were given, and the result was further discussed. Furthermore, the proposed structure was fabricated and measured. The measured results demonstrated the high selectivity, good angular and polarisation stability of the proposed design.
Exogenous damage causes cell DNA damage through mediated reactive oxygen levels
Authors: Guo Peiyan , Ma Ning , Shan Jingbo , Chen Techang , Zhang Yujie , Zhou Sa , Ma Wenjian
Published: November 2019

Many anti-tumor drugs can induce tumor apoptosis by increasing intracellular ROS. In the present study, we build a model which did not directly cause DNA damage, but simulated damage products. The model of this injury was transferred into the cell so that the cell’s damage recognition mechanism mistakenly recognized that its own DNA was damaged, which in turn induced a response. Based on this model, the damaged plasmids (exogenous DNA damage) were transferred into the cells and the amount of reactive oxygen in the cells was improved, and DNA damage of the cells was increased. Therefore, exogenous DNA damage can affect the accumulation of damage in cells by affecting the level of reactive oxygen species, which provides a reference for DNA damage repair research.
Identification of Two Parasitic Nematodes in The Rhizosphere of Centranthera grandiflora in Pingbian, Yunnan
Authors: Fu yan , Chen yuewen , Li wei , Yang Wei , Zhou Yinli
Published: November 2019

Centranthera grandiflora, a kind of traditional Chinese medicinal materials in Yunnan Province, has very high medicinal value. However, constant expansion of the plantation has resulted in worsening root diseases, an obstacle of continuous cropping. From 2018 to 2019, the author investigated the parasitic nematodes in the rhizosphere of Centranthera grandiflora in Pingbian County, Yunnan province, and identified them according to their morphological characteristics. The results showed that there were two kinds of parasitic nematodes harmful to Centranthera grandiflora root in Pingbian County, Yunnan: Tylenchorhynchus capitatus Allen, 1955 and Helicotylenchus dihystera (Cobb, 1893) sher, 1961.
Optimization of the Process of Compound Enzymatic Hydrolysis of Soluble Protease Preparation
Authors: Liu Hao , Lv Chunhui , Liu Peng , Wang Lihui , Wang Peng
Published: November 2019

soluble protease preparations, including trypsin, papain and other preparations, are widely used in various fields, such as medicine, agriculture, industry and so on. Based on this, this paper takes the soluble protease as the main research object, and uses the experimental method of compound enzymolysis to observe and analyze the utilization of egg membrane protein, so as to improve the utilization rate of egg membrane protein.
Stability Analysis of Shallow and Bias Loess Tunnel Based on Finite Difference Method
Authors: Yongbing Li , Li Binglei , Hua Guanyu , Jia Xinran , Chen Yanqiao , Zhang Tanzeng
Published: November 2019

Based on the Mohr-Coulomb elastic-plastic model and the practical engineering background of Mopanshan tunnel, this paper applies the finite-difference software FLAC3D to simulate and analyse the whole process of loess tunnel construction. Then, it analyses the stability of the surrounding rock and sup-port structure after partial excavation of the loess tunnel under the shallow burying and unsymmetrical load-ing condition. The study showed that in the absence of support, the shear failure occurred to the top/upper pilot tunnel of the tunnel face, the failure zone under tensile stress happened to the shallow soil of the earth surface, and the soil of tunnel face appeared to be damaged. Finally, according to the analysis results, a rea-sonable construction method suitable for the shallow and bias loess tunnel is determined.
A Case Study of Low-level Wind Shear in Xining Airport
Authors: Xu Ting , Niu Wei
Published: November 2019

Low-level wind shear is a hazardous phenomenon for aircraft, a low-level wind shear case of Xining airport selected from pilot reports is analysed in this paper. Using ERA-Interim data, the weather pattern and characteristics of wind distribution are discussed. The result indicates cold high pressure accompanied by strong wind and terrain is the main reason of this low-level wind shear case.
The Weakest Point Evaluation Method for the Control Range of the Sound Velocity Profile Station
Authors: Liu Guoqing , Jin Shaohua , You Baoping
Published: November 2019

Sound velocity measurement is an important part of multi-beam sounding, and its accuracy directly affects the coordinate reduction of sounding points. Because of the variability of seawater sound velocity in time and space, the layout density of acoustic section stations directly affects the accuracy of multi-beam sounding. Over-dense layout wastes resources and causes inefficiency; over-sparse layout is not accurate enough to control the entire survey area. Based on the constant gradient acoustic ray tracing algorithm, this paper constructs the accuracy evaluation model of the weakest water depth point of acoustic profile station control, and analyses whether the existing multi-beam acoustic profile station layout scheme can satisfy the accuracy requirements of multi-beam sounding through an example calculation, which provides a theoretical basis for adjusting the layout scheme of acoustic profile station for field surveyors.
Speciation and spatial distribution of nitrogen in surface sediments of in Ming Lake
Authors: Li Yu , Kuang Qi-yu , Din Chao
Published: November 2019

To reveal the diverse occurrence forms and spatial distribution characteristics of nitrogen in the surface sediments of Ming Lake, a sequential leaching method was employed to determine the nitrogen in the surface sediments of Ming Lake. The results show that the total Nitrogen (TN) of the surface sediments of Ming Lake are between 383.23 and 3270.38 ug/g, and the spatial distribution is generally shown as southwest>middle>southeast>west of the lake. The results indicate the nutrient level of the whole lake is not very high. However, for some local areas a risk of high eutrophication pollution may exist due to excessive content; the average TTN is 638.77 ug/g which occupying 36 % of the lake; the spatial distribution is the same with TN; the average (NTN) is 928.61 ug/g that occupied 61 % of TN. With the exception of SAEF-N, every occurrence form of nitrogen was positive with TOM, indicating that the content and spatial distribution of organic matter in the surface sediments of Minghu Lake are the main controlling factors for the content and spatial distribution characteristics of various forms of nitrogen in the sediments.
The investigation of producing bacterial cellulose fibres through hand-spun
Authors: Wang Yu
Published: November 2019

Nanocellulose fibres can be hand-spun from different intermediate states, such as nanocellulose paper and filter cake, which are made from the BC suspension as well as wet pellicle (WP) and dry pellicle (DP) from BC pellicles. In this study, it can be concluded that increasing the hanging weight can increase the Young’s modulus and the tensile strength of fibres. Nanofibres produced from BC pellicles as raw material have better performance than those made from BC suspension. The best properties obtained from the fibres produced from wet pellicles and suspended to a 100g hanging weight upon drying are Young’s modulus (33.8 GPa), tensile strength (610 MPa) and elongation at break (3.6%).
Temporal and Spatial Distribution Pattern of Population at the South End of Hu Line in Recent 25 Years
Authors: Liang Yuting , Yu Huan
Published: November 2019

The southern end of Hu Line mainly involves Sichuan and Yunnan provinces. In this paper, the spatial-temporal distribution pattern of population is analyzed by Standard Deviation Ellipse (SDE) and Exploratory Spatial Data Analysis (ESDA). The influence of human geography, natural geography and soil environmental factors on the spatial distribution of population and interactive interpretation are quantitatively calculated based on Geo-detector. The results show that: (1) the spatial distribution pattern of the population at the southern end of Hu Line is northeast-southwest. The spatial differences between the two sides are significant, the standard deviation ellipse turns weaker gradually, and the population distribution in southwestern China is balanced year by year. (2) The population concentration of the southern end of the Hu Line has been decreasing with the “U” type. Spatial aggregation occurs in high-density counties and surrounding high-density counties. The inaccessible regions appear in contiguous on the west side of the Hu Line. In less counties, there is a clear difference between the surrounding counties and the self, and the spatial distribution of the population tends to be balanced. (3) The dominant factors affecting the spatial distribution of population over the years are GDP density, nighttime lighting, road nuclear density, landforms, farmland production potential and elevation. (4) GDP density, road nuclear density, NDVI and NPP have a good interaction enhancement effect on population distribution. The first geographical intrinsic and the second geographic intrinsic evaluation index have the highest priority. The two-factor action intensity is greater than the single-factor action intensity, and the interaction type tends to be dominated by nonlinear enhancement. The spatial distribution of the population in the southern end of the Hu Line is mainly balanced by economic development and road construction. Night lighting, landform, farmland production potential, elevation, NDVI and NPP are potential factors for optimizing the population distribution at the southern end of the Hu Line.
Prediction of Coalbed Methane Production Based on BP Neural Network
Authors: Zhang Tianxiang , Tang Yifang , Wu Jianjun , Guo Zixi , Li Bing
Published: November 2019

The low average daily gas production per well and the poor economic benefit of exploration and development have become the main problems restricting the exploration and development of coalbed methane in China. Combining multiple coal seam geological parameters to predict the high-yield area of the block can not only provide guidance for the exploitation of coal-bed methane, but also bring enormous economic benefits. Aiming at the difficulty of coalbed methane dessert discrimination and production prediction, a method of coal-bed methane production prediction based on BP neural network is proposed in this paper. Starting from the average daily production of coalbed methane single well, we use the method of grey correlation degree to get the main controlling factors of coalbed methane production. For the main control factors, we use BP neural network with high fitting accuracy and get a good prediction result.
Research on GF-6 Data Selection Technology for Fine Classification of Forest Land
Authors: Deng Guang , Zhang Peng , Li Zhiyong , Tian xin
Published: November 2019

GF-6 satellite is a kind of high-resolution satellites launched by China in recent years. Its sensors have the characteristics of multispectrals, wide field of view, high spatial resolution and high frequency imaging. In order to carry out fine identification of forest types, this paper proposes a method to improve data screening efficiency and data availability rate in GF-6 satellite data selection stage. This paper describes the selection process and key technical methods of GF-6 satellite data, and gives a verification program. It has been proved that the program meets the design objectives and can quickly scree out the required fast screening technologies in the face of massive data and large-area business applications, thus increasing the degree of automation and reducing the workload of manual visual selection.
Application of surveying and mapping technology in airport construction
Authors: Cheng Lan , Chen Fengchen , Tang Ke , Du Wenjia
Published: November 2019

Due to the accelerating urbanization process, land resources are tight, the construction environment becomes more and more complex and severe. In particular, the future airport will be built on the mountain or be constructed reclamation airport, the terrain and geological conditions of the airport construction are complex and the climate is harsh. These all raise higher requirements for the accuracy and safety of airport planning, design and construction. This paper combines the mapping technology with the key links of airport construction, and elaborates on the key technologies, which provides a good reference for related projects and is of great significance for improving the efficiency and quality of airport construction.
Detection of groundwater storage variability based on GRACE and CABLE model in the Murray-Darling Basin
Authors: Li Tongqing , Zhang Qibin , Zhao Yueyao , Gao Yuanbin
Published: November 2019

Monitoring groundwater storage is in great importance for economic and social development. In this paper, the monthly GRACE data from 2003 to 2015 is combined with the Community Atmosphere Biosphere Land Exchange (CABLE) model to estimate the variations of groundwater storage (GWS) in the Murray-Darling Basin (MDB). The results show that (1) the simulations of TWS from CABLE are more accurate than GLDAS over the MDB, and there is a higher correlation coefficient of 0.94 and a lower RMSE of 15.74 between CABLE and GRACE. (3) The spatial pattern of GWS trends shows decline trends in the southwest, east and south, and increasing trends in the north and south central (3) For the whole MDB, the average GWS has strong seasonality and shows an increasing trend with a rate of 1.19 0.41 mmyyear between 2003 and 2015.
Response characteristics of water level dynamic of the Baiyangdian Wetland to climate change and human activities
Authors: Qi Xiaofan , Yin Dechao , An Yonghui , Wang Yushan , Gong Lei
Published: November 2019

Water level dynamics of Wetlands are influenced by both climate change and human activities. Understanding the influence characteristics is important for the management of wetland water resources and ecology. Based on the water level dynamic and precipitation of the Baiyangdian Wetland, and the NPI index, the paper analyzes the response characteristics of the water level dynamic to the precipitation, and the teleconnections between the water level dynamic and the NPI by adopting method of wavelet analysis. Results show that climate change plays an important role on the response of the water level dynamic to the precipitation and also to the NPI, and human activities can significantly change the response characteristics. The response time lags of the water level dynamics to the NPI is longer than that of the water level dynamics to the precipitations, which indicates that the wetland precipitation might be partially influenced by the NPI. The knowledge of the response characteristics obtained in the paper is beneficial for water conservancy and control of the wetland, and is favorable for the sustainable development of its eco-environment.
Effect of hydrogen sulfide(H2S) fumigation on quality of passionfruit (Passiflora edulis sims)
Authors: Liu Yun Fen , Guo Zhen Hong , Song Mu Bo , Liang Yuan Li , Pan Zhong Tian , Duan Zhen Hua , Pu Hong Mei , Shuai Liang , Liao Ling Yan
Published: November 2019

In order to investgate the effects of H2S on the quality of cold-storage passion fruit, fresh harvested fruits were treated with different concentrations of H2S(0 mmol/L, 1 mmol/L, 2 mmol/L, 3 mmol/L), packaged with BOPP plastic wrap and placed into 5±1°C for 35 days. Samples were randomly selected and at 7-days interval during cold storage, compared the preservation effect of different concentrations of H2S. The results indicated that the fruits with 2 mmol/LH2S treatment have better chroma and lower weight loss rate, and soluble solid content, titratable acidity, vitamin C, sugar were preserved at higher level than that of control fruits during storage time. These results showed that 2 mmol/LH2S treatment could maintain the quality of passion fruit during cold storage.
Research on Intelligent Supervision Method and System Developing of Soil Remediation Project Based on 3D Mobile GIS
Authors: Xie Bin , Zhao Xiangwei , Yang Jun , Wang Qingzhong , Pan Shun
Published: November 2019

At present, some problems such as inconvenient manual supervision, lack of real-time online supervision and poor online interaction during the implementation of soil remediation project. In order to solve the problems, the supervision method and system development of soil remediation project are studied based on sensing online and 3D mobile GIS technologies. The remediation environment are monitored with fixed sensors online and the heavy metal content are sampled with the mobile sensors. The status of soil remediation site is supervised in real time with video online. All the real-time sensing data are integrated and stored in the comprehensive database. The information of soil pollution and remediation environment is visualized with maps and charts in 3D geographic scenes in the system. In addition, the pollution degree evaluation and remediation effect analysis functions are implemented in system. It is proved with the project practice that the method and system are convenient for managers to monitor the environment on-site and supervise the status of soil remediation site in real time. The informationization and intelligence level of soil remediation project can be effectively improved.
SWOT Analysis on Promoting GlobalGAP in China’s Agricultural Production in the context of the Belt and Road Initiative
Authors: Li Li-jun , Shi Yi-fei
Published: November 2019

The progress of the Belt and Road Initiative (BRI) has not only created an unprecedented opportunity for the export of China’s agricultural products but also resulted in the opportunity to promote the Global Good Agricultural Practices (GlobalGAP) in China’s agricultural production. In this paper, the SWOT method was adopted to analyze the strengths, weaknesses, opportunities, and threats of promoting GlobalGAP in China’s agricultural production in the context of the BRI.
Higgs physics at CMS
Authors: Dordevic Milos
Published: November 2019

The most recent results of a Higgs boson production and properties from the CMS Collaboration using the LHC Run 1 and Run 2 data are reported. These include analyses of a Higgs boson decaying to a pair of photons, four leptons via Z boson pair decays and the associated production of a Higgs boson with top quark pair, predicted by the Standard Model (SM). The studies of a Higgs boson decays to a pair of bottom quarks, a pair of tau leptons and a charm quark pair are also presented. The Higgs boson production via vector boson fusion (VBF) and decaying to invisible particles is reported here as well. The analysis of a Higgs boson decay to a pair of muons is also presented. The study of a Higgs boson pair production at 13 TeV is performed as well and projections of a Higgs boson self couplings together with the couplings to other particles at the HL-LHC are made.
Overview of hard hadron production results in ALICE
Authors: Peresunko Dmitri
Published: November 2019

The ALICE experiment is designed to study the properties the hot and dense medium, the Quark-Gluon Plasma (QGP), produced in ultrarelativistic heavy-ion collisions at the LHC. Measuring production of hadrons with large Q2 transfer in these collisions provides the possibility to explore one of the most spectacular effects — the in-medium parton energy loss. By varying the observables among light and heavy flavored hadrons and fully reconstructed jets and by changing the colliding systems from pp to p–Pb and Pb–Pb, one can explore the transport properties of hot matter in great details. Here an overview of recent ALICE results on high-pT hadron and jet production in pp, p-A and A-A collisions at LHC energies is presented.
Theoretical Studies of Heavy Ion Collisions via Holography
Authors: Aref’eva Irina
Published: November 2019

Applications of holographic bottom-up QCD models to the studies of Heavy Ion Collisions (HIC) are discussed.
Measurement of the neutral and charged K* (892) mesons in the MPD experiment at NICA
Authors: Ivanishchev Dmitry , Kotov Dmitry , Kryshen Evgeny , Malaev Mikhail , Riabov Victor , Ryabov Yuriy
Published: November 2019

Measurements of K*(892) meson production provide the means to study properties of relativistic heavy-ion collisions. Because of their short lifetimes, the charged and neutral K*(892) mesons provide information about the properties of the late hadronic phase due to the presence of rescattering and regeneration effects that can modify resonance yields. The K*(892) mesons can also be used to study the various mechanisms that shape particle transverse momentum (pT) spectra, including the collective flow, in-medium parton energy loss and recombination. Measurement of neutral K*(892) mesons requires reconstruction of the charged pions and kaons originating from the primary vertex. At the same time, the measurement of charged K*(892) mesons involves reconstruction of the weakly decaying Ks mesons. Simultaneous measurement of the two particles is a way to minimize systematic uncertainties of the corresponding physical studies. We present results of feasibility studies for measurement of the charged and neutral K*(892) mesons in the MPD experimental setup in heavy-ion collisions at NICA energies. Results are obtained using full-scale Monte Carlo simulations of the experimental setup.
Optimization of the input space for deep learning data analysis in HEP.
Authors: Chernoded Andrei , Dudko Lev , Vorotnikov Georgi , Volkov Petr , Ovchinnikov Dmitri , Perfilov Maxim , Shporin Artem
Published: November 2019

Deep learning neural network technique is one of the most efficient and general approach of multivariate data analysis of the collider experiments. The important step of such analysis is the optimization of the input space for multivariate technique. In the article we propose the general recipe how to find the general set of low-level observables sensitive for the differences in the collider hard processes.
Quantum field-theoretical descriprion of neutrino oscillations in T2K experiment
Authors: Egorov Vadim , Rusalev Timofei
Published: November 2019

We consider neutrino oscillations in the T2K experiment using a new quantum field-theoretical approach to the description of processes passing at finite space-time intervals. It is based on the Feynman diagram technique in the coordinate representation, supplemented by modified rules of passing to the momentum representation. Effectively this leads to the Feynman propagators in the momentum representation being modified, while the rest of the Feynman rules remain unchanged. The approach does not make use ofwave packets, the initial and final particle states are described by plane waves, which essentially simplifies the calculations. The oscillation fading out due to momentum distribution of the initial particles is taken into account. The obtained results reproduce the predictions of the standard description and confirm that the far detector position corresponds to the first minimum for muon production probability and the first maximum for electron production probability.
Electromagnetic and gravitomagnetic structure and radii of nucleons
Authors: Selyugin Oleg
Published: November 2019

Taking into account the PDFs, obtained by different Collaborations, the momentum transfer dependenceof GPDs of the nucleons is obtained. The calculated electromagnetic and gravitomagnetic form factors ofnucleons are used for the description of different form factors and the nucleons elastic scattering in a wide energy and momentum transfer region with a minimum number of fitting parameters. The electromagnetic and gravitomagnetic radii of the nucleon are calculated using the obtained momentum transfer dependence of GPDs with different forms of PDFs obtained by different Collaborations. The comparison of the calculations, taking into account the PDFs obtained by different Collaborations, of ean square electromagnetic and gravitomagnetic radii of nucleons is made.
Latest Results in Charmed Baryons Spectroscopy
Authors: Solovieva Elena
Published: November 2019

An overview of recent developments in the spectroscopy of charmed baryons is given. The classification of charmed baryons is presented, a quark model for ground states is briefly described, and the energy levels of excited states are analysed.
New Physics in ultraperipheral collisions at the LHC
Authors: Godunov S.I. , Novikov V. A. , Rozanov A.N. , Vysotsky M.I. , Zhemchugov E.V.
Published: November 2019

We present a model-independent approach for New Physics searches in ultraperipheral collisions at the LHC. The case of long-lived charged particles is considered and it is shown that it is possible to find them even with the already available LHC data.
Molecular Mechanisms of Thyroid Hormone Transport by l-Type Amino
Acid Transporter
Authors: Krause Gerd , Hinz Katrin Manuela
Published: November 2019

Thyroid hormones (TH) pass through the plasma membrane into the target cells via
transporter proteins. Thyroid hormone transporters that have been identified
until now belong to two different solute carrier (SLC) subfamilies i) the major
facilitator superfamily (MFS) and ii) the amino acid polyamine-organocation
(APC) superfamily. Both are comprised by 12 transmembrane helices, however with
different structural topology. The TH transporter MCT8, MCT10 and OATP1C1 are
members of the MSF. The l-type amino acid transporters (LATs) are transporting
neutral amino acids across the membrane. Two LAT subtypes, LAT1 and LAT2 are
members of the APC superfamily, need the escort protein 4F2hc and facilitate
uptake but no efflux of TH-subtypes. Homology models of LAT2 that are based on
crystal structures of APC transporters guided mutagenesis, revealed molecular
structure-function determinants for recognition and transition for import and
export of TH-subtypes. The recently solved cryo-EM structure of LAT1 confirmed
the structural input. Two other LAT subtypes, LAT3 and LAT4 are members of the
MFS. From previous observed negative effect of LAT3 and LAT4 on 3,3’-T2
uptake by LAT1 and LAT2 it was indirectly concluded that LAT3 might export
3,3’-T2. There are still open questions that need to be addressed in
order to fully understand the molecular recognition pattern and traversing
mechanism of import and export of particular TH-subtypes by LAT1 and LAT2.
Moreover, clarification is needed whether LAT3 and LAT4 are exporting TH. Recent
new data could not verify the initial hypothesis of TH export by LAT3.
Therefore, further investigations are necessary to explain the negative effect
of LAT3 on the TH import by LAT2.
Thermal evaporation of pyrene clusters
Authors: Zamith Sébastien , Ji Ming-Chao , L’Hermite Jean-Marc , Joblin Christine , Dontot Léo , Rapacioli Mathias , Spiegelman Fernand
Published: November 2019

This work presents a study of the thermal evaporation and stability of pyrene (C16H10)n clusters. Thermal evaporation rates of positively charged mass-selected clusters are measured for sizes in the range n = 3–40 pyrene units. The experimental setup consists of a gas aggregation source, a thermalization chamber, and a time of flight mass spectrometer. A microcanonical Phase Space Theory (PST) simulation is used to determine the dissociation energies of pyrene clusters by fitting the experimental breakdown curves. Calculations using the Density Functional based Tight Binding combined with a Configuration Interaction (CI-DFTB) model and a hierarchical optimization scheme are also performed in the range n = 2–7 to determine the harmonic frequencies and a theoretical estimation of the dissociation energies. The frequencies are used in the calculations of the density of states needed in the PST simulations, assuming an extrapolation scheme for clusters larger than 7 units. Using the PST model with a minimal set of adjustable parameters, we obtain good fits of the experimental breakdown curves over the full studied size range. The approximations inherent to the PST simulation and the influence of the used parameters are carefully estimated. The derived dissociation energies show significant variations over the studied size range. Compared with neutral clusters, significantly higher values of the dissociation energies are obtained for the smaller sizes and attributed to charge resonance in line with CI-DFTB calculations.
Maintaining low-mode symmetry control with extended pulse shapes for lower-adiabat Bigfoot implosions on the National Ignition Facility
Authors: Hohenberger M. , Casey D. T. , Thomas C. A. , Landen O. L. , Baker K. L. , Benedetti L. R. , Callahan D. A. , Hurricane O. A. , Izumi N. , Khan S. F. , Ma T. , Mariscal D. A. , Nagel S. R. , Pak A. , Spears B. K.
Published: November 2019

The Bigfoot approach to indirect-drive inertial confinement fusion has been developed as a compromise trading high convergence and areal densities for high implosion velocities, large adiabats, and hydrodynamic stability. Shape control and predictability are maintained by using relatively short laser pulses and merging the shocks within the deuterium-tritium-ice layer. These design choices ultimately limit the theoretically achievable performance, and one strategy to increase the 1D performance is to reduce the shell adiabat by extending the pulse shape. However, this can result in the loss of low-mode symmetry control, as the hohlraum “bubble,” the high-Z material launched by the outer-cone beams during the early part of the laser pulse, has more time to expand and will eventually intercept inner-cone beams preventing them from reaching the hohlraum waist, thus losing an equatorial capsule drive. Experiments were performed to study the shape control and predictability with extended pulse shapes in Bigfoot implosions, reducing the adiabat from nominally α∼4 to α∼3 and otherwise very similar experimental parameters. The implosion shape was measured both in-flight and at stagnation, with near-round implosions and low levels of P2 asymmetry throughout, indicating a maintained symmetry control with extended pulse shapes.
Dispersion-driven conformational preference in the gas phase: Microwave spectroscopic and theoretical study of allyl isocyanate
Authors: Sun Wenhao , Sogeke Olamide P. , Silva Weslley G. D. P. , van Wijngaarden Jennifer
Published: November 2019

The conformations of allyl isocyanate (CH2=CHCH2N=C=O) were explored in the gas phase by combining theoretical calculations and Fourier transform microwave spectroscopy, including the chirped pulse and Balle-Flygare types. Three conformers (I, II, and III) were predicted using D3(BJ) dispersion-corrected B3LYP and MP2 methods; however, the lowest energy conformer (conf. I) was absent at the standard B3LYP level. The observed microwave spectra are consistent with the presence of both conf. I and III in the supersonic jet, and surprisingly, this is the first report of the global minimum conf. I both experimentally and theoretically. Rotational transitions from the parent species of both conformers as well as their minor isotopologues (13C, 15N, and 18O) in natural abundance were assigned allowing experimental geometries to be derived. For conf. I, in addition to the typical splitting pattern due to the 14N quadrupole nucleus, the transitions show a tunneling splitting which arises from the interconversion motion between its two mirror images. The experimental observation of conf. I and the absence of conf. II in the jet are rationalized using quantum-chemical calculations to explore the importance of electron correlation and in particular, demonstrate the necessity of including dispersion effects in density functional theory calculations even for seemingly small molecules.