bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental Study on the Inhibition Effects of Nitrogen and Carbon Dioxide on Coal Spontaneous Combustion
Authors: Zhang Yi , Xu Jun , Wang Deming
Published: October 2020

Inert gases can effectively inhibit coal spontaneous combustion. In this paper, the inhibition effect of inert gases (N2 and CO2) on coal spontaneous combustion was studied. In the low-temperature oxidation stage, the constant-temperature heat release and apparent activation energy of coal sample were measured and calculated by the C80 micro-calorimeter. In the high-temperature combustion stage, the critical temperature, maximum peak temperature, ignition temperature, and burn-out temperature of coal samples were analyzed by the synchronous thermal analyzer. The results demonstrate that with the decrease of O2 concentration, the oxidation heat release of coal samples drops gradually while the apparent activation energy increases gradually. In the N2 and CO2 atmospheres, as the O2 concentration is reduced to 1.5% and 3%, respectively, the value of apparent activation energy changes from negative to positive, and the spontaneous reaction transits to a nonspontaneous reaction. The TG-DTG (thermogravimetric-derivative thermogravimetric) curve of coal sample in the high-temperature combustion stage indicates that the critical temperature exhibits a W-shaped trend with the decrease of O2 concentration, which also leads to gradual increases of maximum peak temperature, ignition temperature, and burn-out temperature. The above results signify that increasing the inert gas concentration can gradually reduce the oxidation and combustion rate and improve the inhibition effect on coal spontaneous combustion. In addition, when the O2 concentration is the same, the inhibition effect of CO2 on coal spontaneous combustion is superior to that of N2.
Next-Day Prediction of Hourly Solar Irradiance Using Local Weather Forecasts and LSTM Trained with Non-Local Data
Authors: Jeon Byung-ki , Kim Eui-Jong
Published: October 2020

Solar irradiance prediction is significant for maximizing energy-saving effects in the predictive control of buildings. Several models for solar irradiance prediction have been developed; however, they require the collection of weather data over a long period in the predicted target region or evaluation of various weather data in real time. In this study, a long short-term memory algorithm–based model is proposed using limited input data and data from other regions. The proposed model can predict solar irradiance using next-day weather forecasts by the Korea Meteorological Administration and daily solar irradiance, and it is possible to build a model with one-time learning using national and international data. The model developed in this study showed excellent predictive performance with a coefficient of variation of the root mean square error of 12% per year even if the learning and forecast regions were different, assuming that the weather forecast was correct.
Modified Biochar—A Tool for Wastewater Treatment
Authors: Kopecký Marek , Kolář Ladislav , Konvalina Petr , Strunecký Otakar , Teodorescu Florina , Mráz Petr , Peterka Jiří , Váchalová Radka , Bernas Jaroslav , Bartoš Petr , Filipov Feodor , Bucur Daniel
Published: October 2020

Global deposits of concentrated phosphates, which are a necessary source for the production of phosphate fertilizers, are limited. These reserves keep getting thinner, and every day, large amounts of phosphorus end up in watercourses. In this study, we verified that modified biochar (saturated with FeCl3 solution and then neutralized with NaOH solution) can adsorb significant amounts of phosphorus from wastewater. Moreover, the agrochemical qualities of sludge water from a municipal wastewater treatment plant, struvite, phosphorus-saturated biochar, and iron(III) phosphate from a reused biochar filter were tested in this study. We determined the amount of mobile phosphorus as well as the amount of extractable phosphorus and its five fractions. It was found that modified biochar can hold one-third of the phosphorus amount contained in the commonly used agricultural fertilizer simple superphosphate (1 × 105 g of modified biochar captures up to 2.79 × 103 g of P). Moreover, plants can more easily access phosphorus biochar fractions than struvite, which is formed spontaneously during sludge management. The results of this research prove that the proposed method of recycling phosphorus from wastewater can be applied in technological practice.
Serial Switch Only Rectifier as a Power Conditioning Circuit for Electric Field Energy Harvesting
Authors: Menéndez Oswaldo , Romero Loreto , Cheein Fernando Auat
Published: October 2020

Because traditional electronics cannot directly use the alternating output voltage and current provided by electric field energy harvesters, harvesting systems require additional regulating and conditioning circuits. In this field, this work presents a conditioning circuit, called serial switch-only rectifier (SSOR) for low-voltage electric field energy harvesting (EFEH) applications. The proposed approach consists of a tubular topology harvester mounted on the outer jacket of a 230 V three-wires electrical cable (neutral, ground, and phase), in which terminals are connected to SSOR. We compare SSOR performance with classic electronic approaches, such as a full-bridge rectifier and voltage doubler. Experimental findings showed that the gathered energy by a 1 m cylindrical harvester increased in approximately 73.3% using the SSOR as a power management circuit. Experimental findings showed that the gathered energy by a 1 m cylindrical harvester increase in approximately 73.3% using the SSOR as a power management circuit. This increase is principally due to the fact that a serial bidirectional switch disconnects the harvester from the rest of the management circuit, enhancing the charge collection process. Although simulated results disclosed that SSOR increased collected energy for smaller-scale harvesters (experimental tests obtained using a 10 cm cylindrical harvester), additional losses in bidirectional switch reduced its performance. In addition, we introduce a comprehensive analysis of EFEH systems based on SSOR according to the mains frequency for future power systems.
Thermal and Flow Characteristics in a Concentric Annular Heat Pipe Heat Sink
Authors: Song Eui-Hyeok , Lee Kye-Bock , Rhi Seok-Ho , Kim Kibum
Published: October 2020

A concentric annular heat pipe heat sink (AHPHS) was proposed and fabricated to investigate its thermal behavior. The present AHPHS consists of two concentric pipes of different diameters, which create vacuumed annular vapor space. The main advantage of the AHPHS as a heat sink is that it can largely increase the heat transfer area for cooling compared to conventional heat pipes. In the current AHPHS, condensation takes place along the whole annular space from the certain heating area as the evaporator section. Therefore, the whole inner space of the AHPHS except the heating area can be considered the condenser. In the present study, AHPHSs of different diameters were fabricated and studied experimentally. Basic studies were carried out with a 50 mm-long stainless steel AHPHS with diameter ratios of 1.1 and 1.3 and the same inner tube diameter of 76 mm. Several experimental parameters such as volume fractions of 10–70%, different air flow velocity, flow configurations, and 10–50 W heat inputs were investigated to find their effects on the thermal performance of an AHPHS. Experimental results show that a 10% filling ratio was found to be the optimum charged amount in terms of temperature profile with a low heater surface temperature and water as the working fluid. For the methanol, a 40% filling ratio shows better temperature behavior. Internal working behavior shows not only circular motion but also 3-D flow characteristics moving in axial and circular directions simultaneously.
Diagnoses for Potential Enaction of Water–Energy Nexus in Green Building Rating Systems: Case Study of the Pearl Rating System of United Arab Emirates
Authors: Raveendran Reshna , Hassan Ahmed , Tabet Aoul Kheira Anissa
Published: October 2020

The green building rating system within the sustainability framework of the United Arab Emirates (UAE), the Pearl Rating System (PRS), similar to most international rating systems such as LEED, considers several strategies, regulations, and policies to improve the energy and water performance in buildings. However, the applicability of considering water as part of energy or the fact that the utilization of energy mandates the usage of water seems unexplored and is not yet included in any of the existing building rating systems. A unified approach of water and energy resources is thus vital for future considerations in energy policy, planning, and the inclusion of the same in the sustainability rating systems. This paper investigated, as a case study, the prospects of water–energy nexus in the prevailing UAE green building rating system—PRS—to uncover whether any water conservation strategy has an adverse effect on energy and vice versa. The review revealed that the major shortcomings of the PRS in terms of water–energy nexus strategy are the usage of reference codes that are not suitable for the UAE’s climate and geographical conditions, inexistent synergy between some credit categories, the oversight of rebound effects, and a need for credit reassessment. The paper also recommends that any proposed strategy to realign credit categories in terms of the water–energy nexus with the potential risk to also have a hidden negative rebound effect that researchers and practitioners should identify lest the water–energy tradeoff brings unprecedented repercussions. The theoretical analysis establishes that the bifurcating management of water and energy in the sustainability rating system and energy policy needs to be revisited in order to reap more sustainable and optimum results that are environmentally, ecologically, and financially consistent.
Assessment of Soil Erosion at Multiple Spatial Scales Following Land Use Changes in 1980–2017 in the Black Soil Region, (NE) China
Authors: Fang Haiyan , Fan Zemeng
Published: October 2020

Impact of land use and land cover change on soil erosion is still imperfectly understood, especially in northeastern China where severe soil erosion has occurred since the 1950s. It is important to identify temporal changes of soil erosion for the black soil region at different spatial scales. In the present study, potential soil erosion in northeastern China was estimated based on the Revised Universal Loss Equation by integrating satellite images, and the variability of soil erosion at different spatial scales following land use changes in 1980, 1990, 2000, 2010, and 2017 was analyzed. The regionally spatial patterns of soil loss coincided with the topography, rainfall erosivity, soil erodibility, and use patterns, and around 45% of soil loss came from arable land. Regionally, soil erosion rates increased from 1980 to 2010 and decreased from 2010 to 2017, ranging from 3.91 to 4.45 Mg ha−1 yr−1 with an average of 4.22 Mg ha−1 yr−1 in 1980–2017. Areas with a rate of soil erosion less than 1.41 Mg ha−1 yr−1 decreased from 1980 to 2010 and increased from 2010 to 2017, and the opposite changing patterns occurred in higher erosion classes. Arable land continuously increased at the expense of forest in the high-elevation and steep-slope areas from 1980 to 2010, and decreased from 2010 to 2017, resulting in increased areas with erosion rates higher than 7.05 Mg ha−1 yr−1. At a provincial scale, Liaoning Province experienced the highest soil erosion rate of 9.43 Mg ha−1 yr−1, followed by Jilin Province, the eastern Inner Mongolia Autonomous Region, and Heilongjiang Province. At a county scale, around 75% of the counties had a soil erosion rate higher than the tolerance level. The county numbers with higher erosion rate increased in 1980–2010 and decreased in 2010–2017, resulting from the sprawl and withdrawal of arable land.
Responders to Exercise Therapy in Patients with Osteoarthritis of the Hip: A Systematic Review and Meta-Analysis
Authors: Teirlinck Carolien H. , Verhagen Arianne P. , Reijneveld Elja A.E. , Runhaar Jos , van Middelkoop Marienke , van Ravesteyn Leontien M. , Hermsen Lotte , de Groot Ingrid B. , Bierma-Zeinstra Sita M.A.
Published: October 2020

The Outcome Measures in Rheumatology workgroup (OMERACT), together with the Osteoarthritis Research Society International (OARSI) developed the OMERACT-OARSI responder criteria. These criteria are used to determine if a patient with osteoarthritis (OA) ‘responds’ to therapy, meaning experiences a clinically relevant effect of therapy. Recently, more clinical OA trials report on this outcome and most OA trials have data to calculate the number of responders according to these criteria. A systematic review and meta-analysis were performed on the response to exercise therapy, compared to no or minimal intervention in patients with hip OA using the OMERACT-OARSI responder criteria. The literature was searched for relevant randomized trials. If a trial fit the inclusion criteria, but number of responders was not reported, the first author was contacted. This way the numbers of responders of 14 trials were collected and a meta-analysis on short term (directly after treatment, 12 trials n = 1178) and long term (6–8 months after treatment, six trials n = 519) outcomes was performed. At short term, the risk difference (RD) was 0.14 (95% confidence interval (CI) 0.06–0.22) and number needed to treat (NNT) 7.1 (95% CI 4.5–17); at long term RD was 0.14 (95% CI 0.07–0.20) and NNT 7.1 (95% CI 5.0–14.3). Quality of evidence was moderate for the short term and high for the long term. In conclusion, 14% more hip OA patients responded to exercise therapy than to no therapy.
Cadmium and Lead Levels in Blood and Arsenic Levels in Urine among Schoolchildren Living in Contaminated Glassworks Areas, Sweden
Authors: Mattisson Kristoffer , Tekavec Eva , Lundh Thomas , Stroh Emilie
Published: October 2020

The Kingdom of Crystal, an area in southern Sweden famous for its many glassworks, is historically heavily burdened by pollution from this industry. Glass crust containing cadmium (Cd), lead (Pb), and arsenic (As) has been deposited around the area and used as filling. The purpose of this study was to monitor whether the high levels of metals in the contaminated soil were reflected in blood and urine among school children in this area. Blood and urine samples were collected from 87 children in 2017. The levels of cadmium (Cd-B) and lead (Pb-B) found in blood were determined by inductively coupled plasma mass spectrometry (ICP-MS). The speciation of As in urine (As-U) was performed by ion chromatography. The geometric mean of Cd-B and Pb-B among the children were 0.09 μg/L and 9.9 μg/L respectively. The geometric mean of inorganic As (AsIII and AsV) with metabolites in urine was 6.1 μg/L and 6.94 μg/g creatinine. Children in the study area had blood levels of Pb and Cd that correspond to levels generally found in Swedish children. The levels of inorganic As and its metabolites in urine were low and in the same magnitude as other children in Europe and the U.S.
Public Spaces as Knowledgescapes: Understanding the Relationship between the Built Environment and Creative Encounters at Dutch University Campuses and Science Parks
Authors: Soares Isabelle , Weitkamp Gerd , Yamu Claudia
Published: October 2020

The success of university campuses depends on the interrelations between creative encounters and the built environment, conceptualised here as spatial affordances for creativity. Such an interface plays a fundamental role in interactions for knowledge sharing and the exchange of ideas on campus. Due to campus public spaces generally being considered as the leftovers between buildings and classrooms, undermanaged, and overlooked, little is known about the extent to which this built environment enables or inhibits creative encounters in such spaces. The inner-city campuses and science parks (SPs) of Amsterdam and Utrecht, the case-studies of this research, differ in terms of their location relative to the city, their masterplan typologies and the arrangement of buildings. However, they are similar in terms of the aforementioned issues of public spaces. The novelty of this research is the attempt to overcome such issues using an innovative mixed-methods approach that tests the ‘spatial affordances for creativity’ with empirical data collection and analysis. This raises the importance of mapping, quantifying and analysing the spatial distribution of momentary perceptions, experiences, and feelings of people with methods such as volunteered geographic information (VGI). The results show that proximity between multiple urban functions and physical features, such as parks, cafés and urban seating are important when it comes to explaining the high frequency of creative encounters between people. Urban designers of campuses can use the applied method as a tool to plan and design attractive public spaces that provide creativity through the transfer of tacit knowledge, social well-being, positive momentary perceptions, sense of community, and a sense of place.
Assessment of Relative Asthma Risk in Populations Living near Incineration Facilities in Seoul, Korea
Authors: Bae Hyun-Joo , Kang Jung Eun , Lim Yu-Ra
Published: October 2020

While incineration is among the most commonly used technologies for waste disposal, there is ongoing public concern regarding the adverse health impact. The aim of this study is thus to use health statistics to assess the relative risk of asthma-related hospitalization for those living in close proximity to incineration facilities. We also examine differences in asthma risk related to age demographics. The spatial relationship between incineration facilities and asthma-related hospital admissions in Seoul is analyzed for the period of 2009–2011 using the Rapid Inquiry Facility (RIF) and SaTScan software. The relative risk of asthma-related hospitalization decreased with increasing distance from incinerators, but increased among those living within a 2-km radius. The relative risks of asthma-related hospitalization were 1.13 (95% confidence interval (CI): 1.10–1.17), 1.12 (95% CI: 1.08–1.17), and 1.18 (95% CI: 1.10–1.27) for all ages, those aged below 15 years, and those aged 65 years and older, respectively. This study is the first to observe an increased risk of asthma-related hospitalization in relation to a person’s distance from an incinerator in Seoul, Korea. It is clear that asthma should be considered an adverse health outcome during health impact assessments of incineration plants.
Hypertension Prevalence and Determinants among Black South African Adults in Semi-Urban and Rural Areas
Authors: Mphekgwana Peter M. , Malema Nancy , Monyeki Kotsedi D. , Mothiba Tebogo M. , Makgahlela Mpsanyana , Kgatla Nancy , Makgato Irene , Sodi Tholene
Published: October 2020

The burden of hypertension is reported to be on the rise in developing countries, such as South Africa, despite increased efforts to address it. Using a cross-sectional study design, we assessed and compared the prevalence of and risk factors associated with hypertension amongst adults aged ≥18 years in semi-urban and rural communities (1187 semi-urban and 1106 rural). Trained community health workers administered the INTERHEART Risk Score tool and performed blood pressure assessments using the MEDIC Pharmacists Choice Blood Pressure Monitor. Hypertension was defined to be a systolic blood pressure (BP) ≥ 140 mmHg and diastolic BP ≥ 90 mmHg. A multivariate logistic regression model was used to identify factors and determine their relationship with hypertension. The prevalence of hypertension amongst semi-urban and rural communities was 21% with no gender difference. In the semi-urban area, physical activity, family history, fruit intake, salty food, and eating meat were significantly associated with the odds of hypertension among women, whereas only the waist-to-hip ratio (WHR), diabetic status, and salty food were the predictors for rural women. Factors such as fried food and low fruit intake were significantly associated with the odds of hypertension among men in the semi-urban area, whereas only the WHR was significant among men in the rural area. Hypertension was found to be prevalent among semi-urban and rural adults in Limpopo Province, South Africa.
Assessment of Social Distancing for Controlling COVID-19 in Korea: An Age-Structured Modeling Approach
Authors: Choi Yongin , Kim James Slghee , Choi Heejin , Lee Hyojung , Lee Chang Hyeong
Published: October 2020

The outbreak of the novel coronavirus disease 2019 (COVID-19) occurred all over the world between 2019 and 2020. The first case of COVID-19 was reported in December 2019 in Wuhan, China. Since then, there have been more than 21 million incidences and 761 thousand casualties worldwide as of 16 August 2020. One of the epidemiological characteristics of COVID-19 is that its symptoms and fatality rates vary with the ages of the infected individuals. This study aims at assessing the impact of social distancing on the reduction of COVID-19 infected cases by constructing a mathematical model and using epidemiological data of incidences in Korea. We developed an age-structured mathematical model for describing the age-dependent dynamics of the spread of COVID-19 in Korea. We estimated the model parameters and computed the reproduction number using the actual epidemiological data reported from 1 February to 15 June 2020. We then divided the data into seven distinct periods depending on the intensity of social distancing implemented by the Korean government. By using a contact matrix to describe the contact patterns between ages, we investigated the potential effect of social distancing under various scenarios. We discovered that when the intensity of social distancing is reduced, the number of COVID-19 cases increases; the number of incidences among the age groups of people 60 and above increases significantly more than that of the age groups below the age of 60. This significant increase among the elderly groups poses a severe threat to public health because the incidence of severe cases and fatality rates of the elderly group are much higher than those of the younger groups. Therefore, it is necessary to maintain strict social distancing rules to reduce infected cases.
Oncolytic Viruses as a Platform for the Treatment of Malignant Brain Tumors
Authors: Sostoa Jana de , Dutoit Valérie , Migliorini Denis
Published: October 2020

Malignant brain tumors remain incurable diseases. Although much effort has been devoted to improving patient outcome, multiple factors such as the high tumor heterogeneity, the strong tumor-induced immunosuppressive microenvironment, and the low mutational burden make the treatment of these tumors especially challenging. Thus, novel therapeutic strategies are urgent. Oncolytic viruses (OVs) are biotherapeutics that have been selected or engineered to infect and selectively kill cancer cells. Increasingly, preclinical and clinical studies demonstrate the ability of OVs to recruit T cells and induce durable immune responses against both virus and tumor, transforming a “cold” tumor microenvironment into a “hot” environment. Besides promising clinical results as a monotherapy, OVs can be powerfully combined with other cancer therapies, helping to overcome critical barriers through the creation of synergistic effects in the fight against brain cancer. Although many questions remain to be answered to fully exploit the therapeutic potential of OVs, oncolytic virotherapy will clearly be part of future treatments for patients with malignant brain tumors.
Proteome Analysis of Walnut Bacterial Blight Disease
Authors: H. D. Sagawa Cíntia , de A. B. Assis Renata , Zaini Paulo A. , Wilmarth Phillip A. , Phinney Brett S. , Moreira Leandro M. , Dandekar Abhaya M.
Published: October 2020

The interaction between the plant host, walnut (Juglans regia; Jr), and a deadly pathogen (Xanthomonas arboricola pv. juglandis 417; Xaj) can lead to walnut bacterial blight (WB), which depletes walnut productivity by degrading the nut quality. Here, we dissect this pathosystem using tandem mass tag quantitative proteomics. Walnut hull tissues inoculated with Xaj were compared to mock-inoculated tissues, and 3972 proteins were identified, of which 3296 are from Jr and 676 from Xaj. Proteins with differential abundance include oxidoreductases, proteases, and enzymes involved in energy metabolism and amino acid interconversion pathways. Defense responses and plant hormone biosynthesis were also increased. Xaj proteins detected in infected tissues demonstrate its ability to adapt to the host microenvironment, limiting iron availability, coping with copper toxicity, and maintaining energy and intermediary metabolism. Secreted proteases and extracellular secretion apparatus such as type IV pilus for twitching motility and type III secretion effectors indicate putative factors recognized by the host. Taken together, these results suggest intense degradation processes, oxidative stress, and general arrest of the biosynthetic metabolism in infected nuts. Our results provide insights into molecular mechanisms and highlight potential molecular tools for early detection and disease control strategies.
Dissecting Cellular Mechanisms of Long-Chain Acylcarnitines-Driven Cardiotoxicity: Disturbance of Calcium Homeostasis, Activation of Ca2+-Dependent Phospholipases, and Mitochondrial Energetics Collapse
Authors: Berezhnov Alexey V. , Fedotova Evgeniya I. , Nenov Miroslav N. , Kasymov Vitaly A. , Pimenov Oleg Yu. , Dynnik Vladimir V.
Published: October 2020

Long-chain acylcarnitines (LCAC) are implicated in ischemia-reperfusion (I/R)-induced myocardial injury and mitochondrial dysfunction. Yet, molecular mechanisms underlying involvement of LCAC in cardiac injury are not sufficiently studied. It is known that in cardiomyocytes, palmitoylcarnitine (PC) can induce cytosolic Ca2+ accumulation, implicating L-type calcium channels, Na+/Ca2+ exchanger, and Ca2+-release from sarcoplasmic reticulum (SR). Alternatively, PC can evoke dissipation of mitochondrial potential (ΔΨm) and mitochondrial permeability transition pore (mPTP). Here, to dissect the complex nature of PC action on Ca2+ homeostasis and oxidative phosphorylation (OXPHOS) in cardiomyocytes and mitochondria, the methods of fluorescent microscopy, perforated path-clamp, and mitochondrial assays were used. We found that LCAC in dose-dependent manner can evoke Ca2+-sparks and oscillations, long-living Ca2+ enriched microdomains, and, finally, Ca2+ overload leading to hypercontracture and cardiomyocyte death. Collectively, PC-driven cardiotoxicity involves: (I) redistribution of Ca2+ from SR to mitochondria with minimal contribution of external calcium influx; (II) irreversible inhibition of Krebs cycle and OXPHOS underlying limited mitochondrial Ca2+ buffering; (III) induction of mPTP reinforced by PC-calcium interplay; (IV) activation of Ca2+-dependent phospholipases cPLA2 and PLC. Based on the inhibitory analysis we may suggest that simultaneous inhibition of both phospholipases could be an effective strategy for protection against PC-mediated toxicity in cardiomyocytes.
The Diverse Roles of TAO Kinases in Health and Diseases
Authors: Fang Chih-Yeu , Lai Tsung-Ching , Hsiao Michael , Chang Yu-Chan
Published: October 2020

Thousand and one kinases (TAOKs) are members of the MAP kinase kinase kinase (MAP3K) family. Three members of this subfamily, TAOK1, 2, and 3, have been identified in mammals. It has been shown that TAOK1, 2 and 3 regulate the p38 MAPK and Hippo signaling pathways, while TAOK 1 and 2 modulate the SAPK/JNK cascade. Furthermore, TAOKs are involved in additional interactions with other cellular proteins and all of these pathways modulate vital physiological and pathophysiological responses in cells and tissues. Dysregulation of TAOK-related pathways is implicated in the development of diseases including inflammatory and immune disorders, cancer and drug resistance, and autism and Alzheimer’s diseases. This review collates current knowledge concerning the roles of TAOKs in protein–protein interaction, signal transduction, physiological regulation, and pathogenesis and summarizes the recent development of TAOK-specific inhibitors that have the potential to ameliorate TAOKs’ effects in pathological situations.
Exploring the Applications of the Photoprotective Properties of Anthocyanins in Biological Systems
Authors: Oliveira Hélder , Correia Patrícia , Pereira Ana Rita , Araújo Paula , Mateus Nuno , de Freitas Victor , Oliveira Joana , Fernandes Iva
Published: October 2020

Due to their physical and chemical characteristics, anthocyanins are amongst the most versatile groups of natural compounds. Such unique signature makes these compounds a focus in several different areas of research. Anthocyanins have well been reported as bioactive compounds in a myriad of health disorders such as cardiovascular diseases, cancer, and obesity, among others, due to their anti-inflammatory, antioxidant, anti-diabetic, anti-bacterial, and anti-proliferative capacities. Such a vast number of action mechanisms may be also due to the number of structurally different anthocyanins plus their related derivatives. In this review, we highlight the recent advances on the potential use of anthocyanins in biological systems with particular focus on their photoprotective properties. Topics such as skin aging and eye degenerative diseases, highly influenced by light, and the action of anthocyanins against such damages will be discussed. Photodynamic Therapy and the potential role of anthocyanins as novel photosensitizers will be also a central theme of this review.
The BDNF rs6265 Polymorphism is a Modifier of Cardiomyocyte Contractility and Dilated Cardiomyopathy
Authors: Raucci Frank J. , Singh Anand Prakash , Soslow Jonathan , Markham Larry W. , Zhong Lin , Aljafar Wejdan , Lessiohadi Natasja , Awgulewitsch Cassandra P. , Umbarkar Prachi , Zhang Qinkun , Cannon Presley L. , Buchowski Maciej , Roland Joseph T. , Carrier Erica J. , Burnette William B. , Hatzopoulos Antonis K. , Lal Hind , Galindo Cristi L.
Published: October 2020

Brain-derived neurotrophic factor (BDNF) is a neuronal growth and survival factor that harbors cardioprotective qualities that may attenuate dilated cardiomyopathy. In ~30% of the population, BDNF has a common, nonsynonymous single nucleotide polymorphism rs6265 (Val66Met), which might be correlated with increased risk of cardiovascular events. We previously showed that BDNF correlates with better cardiac function in Duchenne muscular dystrophy (DMD) patients. However, the effect of the Val66Met polymorphism on cardiac function has not been determined. The goal of the current study was to determine the effects of rs6265 on BDNF biomarker suitability and DMD cardiac functions more generally. We assessed cardiovascular and skeletal muscle function in human DMD patients segregated by polymorphic allele. We also compared echocardiographic, electrophysiologic, and cardiomyocyte contractility in C57/BL-6 wild-type mice with rs6265 polymorphism and in mdx/mTR (mDMD) mouse model of DMD. In human DMD patients, plasma BDNF levels had a positive correlation with left ventricular function, opposite to that seen in rs6265 carriers. There was also a substantial decrease in skeletal muscle function in carriers compared to the Val homozygotes. Surprisingly, the opposite was true when cardiac function of DMD carriers and non-carriers were compared. On the other hand, Val66Met wild-type mice had only subtle functional differences at baseline but significantly decreased cardiomyocyte contractility. Our results indicate that the Val66Met polymorphism alters myocyte contractility, conferring worse skeletal muscle function but better cardiac function in DMD patients. Moreover, these results suggest a mechanism for the relative preservation of cardiac tissues compared to skeletal muscle in DMD patients and underscores the complexity of BDNF signaling in response to mechanical workload.
Towards an Integral Therapeutic Protocol for Breast Cancer Based upon the New H+-Centered Anticancer Paradigm of the Late Post-Warburg Era
Authors: Harguindey Salvador , Alfarouk Khalid , Polo Orozco Julián , Fais Stefano , Devesa Jesús
Published: October 2020

A brand new approach to the understanding of breast cancer (BC) is urgently needed. In this contribution, the etiology, pathogenesis, and treatment of this disease is approached from the new pH-centric anticancer paradigm. Only this unitarian perspective, based upon the hydrogen ion (H+) dynamics of cancer, allows for the understanding and integration of the many dualisms, confusions, and paradoxes of the disease. The new H+-related, wide-ranging model can embrace, from a unique perspective, the many aspects of the disease and, at the same time, therapeutically interfere with most, if not all, of the hallmarks of cancer known to date. The pH-related armamentarium available for the treatment of BC reviewed here may be beneficial for all types and stages of the disease. In this vein, we have attempted a megasynthesis of traditional and new knowledge in the different areas of breast cancer research and treatment based upon the wide-ranging approach afforded by the hydrogen ion dynamics of cancer. The concerted utilization of the pH-related drugs that are available nowadays for the treatment of breast cancer is advanced.
Salivary Biomarkers for Diagnosis of Inflammatory Bowel Diseases: A Systematic Review
Authors: Nijakowski Kacper , Surdacka Anna
Published: October 2020

Saliva as a biological fluid has a remarkable potential in the non-invasive diagnostics of several systemic disorders. Inflammatory bowel diseases are chronic inflammatory disorders of the gastrointestinal tract. This systematic review was designed to answer the question “Are salivary biomarkers reliable for the diagnosis of inflammatory bowel diseases?”. Following the inclusion and exclusion criteria, eleven studies were included (according to PRISMA statement guidelines). Due to their heterogeneity, the potential salivary markers for IBD were divided into four groups: oxidative status markers, inflammatory cytokines, microRNAs and other biomarkers. Active CD patients manifest decreased activity of antioxidants (e.g., glutathione, catalase) and increased lipid peroxidation. Therefore, malondialdehyde seems to be a good diagnostic marker of CD. Moreover, elevated concentrations of proinflammatory cytokines (such as interleukin 1β, interleukin 6 or tumour necrosis factor α) are associated with the activity of IBD. Additionaly, selected miRNAs are altered in saliva (overexpressed miR-101 in CD; overexpressed miR-21, miR-31, miR-142-3p and underexpressed miR-142-5p in UC). Among other salivary biomarkers, exosomal PSMA7, α-amylase and calprotectin are detected. In conclusion, saliva contains several biomarkers which can be used credibly for the early diagnosis and regular monitoring of IBD. However, further investigations are necessary to validate these findings, as well as to identify new reliable salivary biomarkers.
Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells
Authors: Feliksiak Karolina , Witko Tomasz , Solarz Daria , Guzik Maciej , Rajfur Zenon
Published: October 2020

Vimentin, an intermediate filament protein present in leukocytes, blood vessel endothelial cells, and multiple mesenchymal cells, such as mouse embryonic fibroblasts (MEF 3T3), is crucial for various cellular processes, as well as for maintaining the integrity and durability (stability) of the cell cytoskeleton. Vimentin intermediate filaments (VIFs) adhere tightly to the nucleus and spread to the lamellipodium and tail of the cell, serving as a connector between the nucleus, and the cell’s edges, especially in terms of transferring mechanical signals throughout the cell. How these signals are transmitted exactly remains under investigation. In the presented work, we propose that vimentin is involved in that transition by influencing the shape of the nucleus through the formation of nuclear blebs and grooves, as demonstrated by microscopic observations of healthy MEF (3T3) cells. Grooved, or “coffee beans” nuclei, have, to date, been noticed in several healthy cells; however, these structures are especially frequent in cancer cells—they serve as a significant marker for recognition of multiple cancers. We observed 288 MEF3T3 cells cultured on polyhydroxyoctanoate (PHO), polylactide (PLA), and glass, and we identified grooves, coaligned with vimentin fibers in the nuclei of 47% of cells cultured on PHO, 50% of cells on glass, and 59% of cells growing on PLA. We also observed nuclear blebs and associated their occurrence with the type of substrate used for cell culture. We propose that the higher rate of blebs in the nuclei of cells, cultured on PLA, is related to the microenvironmental features of the substrate, pH in particular.
Long Non-Coding RNAs as Regulators of Interactions between Cancer-Associated Fibroblasts and Cancer Cells in the Tumor Microenvironment
Authors: Ahn Young-Ho , Kim Jeong Seon
Published: October 2020

Long non-coding RNAs (lncRNAs) regulate diverse physiological and pathological processes via post-transcriptional, post-translational, and epigenetic mechanisms. They are also involved in tumor initiation, progression, and metastasis by functioning as key players in the tumor microenvironment. Cancer-associated fibroblasts (CAFs) promote tumor initiation, progression, metastasis, drug resistance, and immunosuppression, which can be modulated by lncRNAs. LncRNAs regulate the intrinsic properties of CAFs or cancer cells intracellularly or function extracellularly through exosomal secretion. In-depth studies on the mechanisms of lncRNA functions will enable their clinical use as diagnosis/prognosis markers and therapeutic targets in cancer treatment.
Transcriptome and Gene Editing Analyses Reveal MOF1a Defect Alters the Expression of Genes Associated with Tapetum Development and Chromosome Behavior at Meiosis Stage Resulting in Low Pollen Fertility of Tetraploid Rice
Authors: Lu Zijun , Guo Xiaotong , Huang Zhiyu , Xia Juan , Li Xiang , Wu Jinwen , Yu Hang , Shahid Muhammad Qasim , Liu Xiangdong
Published: October 2020

Autotetraploid rice is a useful rice germplasm for polyploid rice breeding. However, low fertility limits its commercial production. A neo-tetraploid rice with high fertility was developed from the progenies of crossing between autotetraploid lines by our research group. Our previous study showed that a myeloblastosis (MYB) transcription factor, MOF1, might be associated with the pollen development in tetraploid rice. However, little information is available about its role in pollen development in tetraploid rice. Here, we identified a new haplotype of MOF1 from neo-tetraploid rice and marked it as MOF1a. Transcriptome and qRT-PCR analysis demonstrated that MOF1a highly expressed in anthers, and displayed differential expression in neo-tetraploid rice compared to tetraploid rice line with low pollen fertility. The mutant (mof1a) of MOF1a, which was generated by CRISPR/Cas9 knockout in neo-tetraploid rice, showed low pollen fertility, and also exhibited abnormal tapetum and middle layer development, and defective chromosome behaviors during meiosis. A total of 13 tapetal related genes were found to be up-regulated in meiotic anthers of MOF1a compared with wild type plants by RNA-seq analysis, including CYP703A3, PTC1, and OsABCG26, which had been demonstrated to affect tapetal development. Moreover, 335 meiosis-related genes displayed differential expression patterns at same stage, including nine important meiosis-related genes, such as metallothionein OsMT1a. These results demonstrated that MOF1a plays an important role in pollen development and provides a foundation for understanding the molecular mechanism underlying MOF1a in reproduction of tetraploid rice.
Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants
Authors: Zhang Lin , Zhang Hanwen , Yang Shushen
Published: October 2020

Drought is a major natural disaster that seriously affects agricultural production, especially for winter wheat in boreal China. As functional proteins, the functions and mechanisms of glyceraldehyde-3-phosphate dehydrogenase in cytoplasm (GAPCs) have remained little investigated in wheat subjected to adverse environmental conditions. In this study, we cloned and characterized a GAPC isoform TaGAPC2 in wheat. Over-expression of TaGApC2-6D in Arabidopsis led to enhanced root length, reduced reactive oxygen species (ROS) production, and elevated drought tolerance. In addition, the dual-luciferase assays showed that TaWRKY28/33/40/47 could positively regulate the expression of TaGApC2-6A and TaGApC2-6D. Further results of the yeast two-hybrid system and bimolecular fluorescence complementation assay (BiFC) demonstrate that TaPLDδ, an enzyme producing phosphatidic acid (PA), could interact with TaGAPC2-6D in plants. These results demonstrate that TaGAPC2 regulated by TaWRKY28/33/40/47 plays a crucial role in drought tolerance, which may influence the drought stress conditions via interaction with TaPLDδ. In conclusion, our results establish a new positive regulation mechanism of TaGAPC2 that helps wheat fine-tune its drought response.
Long-Chain Saturated Fatty Acids, Palmitic and Stearic Acids, Enhance the Repair of Photosystem II
Authors: Jimbo Haruhiko , Takagi Kensuke , Hirashima Takashi , Nishiyama Yoshitaka , Wada Hajime
Published: October 2020

Free fatty acids (FFA) generated in cyanobacterial cells can be utilized for the biodiesel that is required for our sustainable future. The combination of FFA and strong light induces severe photoinhibition of photosystem II (PSII), which suppresses the production of FFA in cyanobacterial cells. In the present study, we examined the effects of exogenously added FFA on the photoinhibition of PSII in Synechocystis sp. PCC 6803. The addition of lauric acid (12:0) to cells accelerated the photoinhibition of PSII by inhibiting the repair of PSII and the de novo synthesis of D1. α-Linolenic acid (18:3) affected both the repair of and photodamage to PSII. Surprisingly, palmitic (16:0) and stearic acids (18:0) enhanced the repair of PSII by accelerating the de novo synthesis of D1 with the mitigation of the photoinhibition of PSII. Our results show chemical potential of FFA in the regulation of PSII without genetic manipulation.
RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (Schistocerca gregaria) Inhibits Adult Morphogenesis and Results in Supernumerary Juvenile Stages
Authors: Gijbels Marijke , Marchal Elisabeth , Verdonckt Thomas Wolf , Bruyninckx Evert , Vanden Broeck Jozef
Published: October 2020

Postembryonic development of insects is coordinated by juvenile hormone (JH) together with ecdysteroids. Whereas the JH early response gene krüppel-homolog 1 (Kr-h1) plays a crucial role in the maintenance of juvenile characteristics during consecutive larval stages, the ecdysteroid-inducible early gene E93 appears to be a key factor promoting metamorphosis and adult morphogenesis. Here, we report on the developmental and molecular consequences of an RNAi-mediated knockdown of SgE93 in the desert locust, Schistocerca gregaria, a hemimetabolan species. Our experimental data show that injection of gregarious locust nymphs with a double-stranded RNA construct targeting the SgE93 transcript inhibited the process of metamorphosis and instead led to supernumerary nymphal stages. These supernumerary nymphal instars still displayed juvenile morphological features, such as a nymphal color scheme and body shape, while they reached the physical body size of the adult locusts, or even surpassed it after the next supernumerary molt. Interestingly, when compared to control locusts, the total duration of the fifth and normally final nymphal (N5) stage was shorter than normal. This appeared to correspond with temporal and quantitative changes in hemolymph ecdysteroid levels, as well as with altered expression of the rate-limiting Halloween gene, Spook (SgSpo). In addition, the levels of the ecdysone receptor (SgEcR) and retinoïd X receptor (SgRXR) transcripts were altered, indicating that silencing SgE93 affects both ecdysteroid synthesis and signaling. Upon knockdown of SgE93, a very potent upregulation of the SgKr-h1 transcript levels was observed in both head and fat body, while no significant changes were detected in the transcript levels of SgJHAMT and SgCYP15A1, the enzymes that catalyze the two final steps in JH biosynthesis. Moreover, the process of molting was disturbed in these supernumerary nymphs. While attempting ecdysis to the next stage, 50% of the N6 and all N7 nymphal instars eventually died. S. gregaria is a very harmful, swarm-forming pest species that destroys crops and threatens food security in many of the world’s poorest countries. We believe that a better knowledge of the mechanisms of postembryonic development may contribute to the discovery of novel, more selective and sustainable strategies for controlling gregarious locust populations. In this context, identification of molecular target candidates that are capable of significantly reducing the fitness of this devastating swarming pest will be of crucial importance.
ALS-Related Mutant SOD1 Aggregates Interfere with Mitophagy by Sequestering the Autophagy Receptor Optineurin
Authors: Tak Yeong Jin , Park Ju-Hwang , Rhim Hyangshuk , Kang Seongman
Published: October 2020

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the progressive demise of motor neurons. One of the causes of familial ALS is the mutation of the gene encoding superoxide dismutase 1 (SOD1), which leads to abnormal protein aggregates. How SOD1 aggregation drives ALS is still poorly understood. Recently, ALS pathogenesis has been functionally implicated in mitophagy, specifically the clearance of damaged mitochondria. Here, to understand this mechanism, we investigated the relationship between the mitophagy receptor optineurin and SOD1 aggregates. We found that mutant SOD1 (mSOD1) proteins associate with and then sequester optineurin, which is required to form the mitophagosomes, to aggregates in N2a cells. Optineurin recruitment into mSOD1 aggregates resulted in a reduced mitophagy flux. Furthermore, we observed that an exogenous augmentation of optineurin alleviated the cellular cytotoxicity induced by mSOD1. Taken together, these studies demonstrate that ALS-linked mutations in SOD1 interfere with the mitophagy process through optineurin sequestration, suggesting that the accumulation of damaged mitochondria may play a crucial role in the pathophysiological mechanisms contributing to ALS.
PAK1 Regulates MEC-17 Acetyltransferase Activity and Microtubule Acetylation during Proplatelet Extension
Authors: van Dijk Juliette , Bompard Guillaume , Rabeharivelo Gabriel , Cau Julien , Delsert Claude , Morin Nathalie
Published: October 2020

Mature megakaryocytes extend long processes called proplatelets from which platelets are released in the blood stream. The Rho GTPases Cdc42 and Rac as well as their downstream target, p21-activated kinase 2 (PAK2), have been demonstrated to be important for platelet formation. Here we address the role, during platelet formation, of PAK1, another target of the Rho GTPases. PAK1 decorates the bundled microtubules (MTs) of megakaryocyte proplatelets. Using a validated cell model which recapitulates proplatelet formation, elongation and platelet release, we show that lack of PAK1 activity increases the number of proplatelets but restrains their elongation. Moreover, in the absence of PAK1 activity, cells have hyperacetylated MTs and lose their MT network integrity. Using inhibitors of the tubulin deacetylase HDAC6, we demonstrate that abnormally high levels of MT acetylation are not sufficient to increase the number of proplatelets but cause loss of MT integrity. Taken together with our previous demonstration that MT acetylation is required for proplatelet formation, our data reveal that MT acetylation levels need to be tightly regulated during proplatelet formation. We identify PAK1 as a direct regulator of the MT acetylation levels during this process as we found that PAK1 phosphorylates the MT acetyltransferase MEC-17 and inhibits its activity.