bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Oenological Fermentation: Which Strategy and Which Tools?
Authors: Mouret Jean-Roch , Aguera Evelyne , Perez Marc , Farines Vincent , Sablayrolles Jean-Marie
Published: August 2021

Wine fermentation is a specific and complex research subject and its control is essential to ensure full process completion while improving wine quality. It displays several specificities, in particular, (i) musts with a very high sugar content, low pH, and some limiting nutrients, as well as a great variability in must composition according to the year, grape variety, and so on; (ii) atypical fermentation conditions with non-isothermal temperature profiles, a quasi-anaerobiosis and legal constraints with a limited and predefined list of authorized operations. New challenges have emerged, related to the increasing diversity of commercially available yeast strains; the fluctuating composition of musts, particularly owing to climate change; and sustainability, which has become a key issue. This paper synthesizes approaches implemented to address all these issues. It details the example of our laboratory that, for many years, has been developing an integrated approach to study yeast diversity, understand their metabolism, and develop new fermentation control strategies. This approach requires the development of specific fermentation devices to study yeast metabolism in a controlled environment that mimics practical conditions and to develop original fermentation control strategies. All these tools are described here, together with their role in the overall scientific strategy and complementary approaches in the literature.
A Historical Perspective of Landscape and Human Population Dynamics in Guimarães (Northern Portugal): Possible Implications of Rural Fire Risk in a Changing Environment
Authors: Nunes Leonel , Raposo Mauro , Pinto Gomes Carlos
Published: August 2021

The occupation of a territory combines a set of variables which affect the development of the mode by which populations have been organized throughout history. How this occupation takes place demonstrates much of a territory’s past and shows how the populations managed to make the most out of the available resources. The region of Entre-Douro-e-Minho (Northern Portugal), similarly to what happens in other regions, such as Galicia (Northern Spain), Brittany (Northern France), or Ireland, presents a type of dispersed land use, with an alternation of urban, agriculture, and forest areas. On one hand, this proximity allows urban populations to come into contact with a rural environment. On the other hand, this proximity also causes a set of problems, namely those related to rural fires, which are now enhanced by climate change, and associated phenomena, such as heatwaves and the lack of precipitation. The present work analyzes the evolution of rural fires in 1975–2019, in the municipality of Guimarães (Northern Portugal), to understand how these events have been distributed over time and evolved in a climate change scenario. Based on the results and discussion presented, it can be concluded that there is an increasing trend in the occurrence of rural fires in the territory under study, and that this can also be associated to climate change, in the form of a gradual increment in temperature, particularly in the autumn months, and a decrease in rainfall. This situation is responsible for the increment of the risk caused by the proximity of the populations to forest and agricultural areas because rural fires can jeopardize the safety of people and goods.
Trends in Food Preferences and Sustainable Behavior during the COVID-19 Lockdown: Evidence from Spanish Consumers
Authors: Li Shanshan , Kallas Zein , Rahmani Djamel , Gil José Maria
Published: August 2021

The COVID-19 pandemic poses a threat to global food security, and it changes consumers’ food buying and consumption behavior. This research not only investigates trends in Spanish consumers’ general food shopping and consumption habits during the lockdown, but also investigates these trends from the perspective of sustainable purchasing. Specifically, total food consumption (C), food expenditure (E), and purchase of food with sustainable attributes (S) were measured. Data were collected from a semi-structured questionnaire which was distributed online among 1203 participants. The logit models showed that gender, age, employment status, and consumers’ experiences were associated with total food consumption and expenditure during the lockdown. In addition, consumers’ risk perceptions, shopping places, trust level in information sources, and risk preference were highly essential factors influencing consumers’ preferences and sustainable behavior. Consumers’ objective knowledge regarding COVID-19 was related to expenditure. Furthermore, family structure only affected expenditure, while income and place of residence influenced food consumption. Mood was associated with expenditure and the purchase of sustainable food. Household size affected purchasing behavior towards food with sustainable attributes. This research provides references for stakeholders that help them to adapt to the new COVID-19 situation.
Fatty Acid Composition of Cannabis sativa, Linum usitatissimum and Camelina sativa Seeds Harvested in Lithuania for Food Use
Authors: Razmaitė Violeta , Pileckas Vidmantas , Bliznikas Saulius , Šiukščius Artūras
Published: August 2021

The objective of this study was to determine species-associated differences in the seed proximate and fatty acid composition of three traditional oil crop species, hemp (Cannabis sativa), flax (Linum usitatissimum), and camelina (Camelina sativa), and the sowing season of camelina harvested under Lithuanian farming conditions for food use. Camelina seeds had the highest (p < 0.001) content of protein, oil, and sugar contents compared to both dehulled hemp and flax seeds. The amounts of protein and oil in camelina seeds were considerably increased by their summer cultivar, which showed higher (p < 0.001) contents of protein and oil than winter cultivars. However, the highest and lowest (p < 0.001) fiber content was found in flax seeds and camelina seeds, respectively. Camelina seeds showed considerably higher and lower (p < 0.001) proportions of total monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids, respectively, compared with hemp and flax. The summer cultivar of camelina had higher (p < 0.001) proportions of saturated (SFA) and MUFA and lower proportions of PUFA compared with winter cultivars. Hemp seeds had the highest and lowest (p < 0.001) proportions of PUFA and MUFA, respectively. The n-6/n-3 PUFA ratio in hemp seeds is optimal (3.79), whereas the use of flax and camelina seeds with their n-6/n-3 ratios of 0.28 and 0.48, respectively, can significantly improve this ratio in the overall diet. The properties of oil crop seeds showed that whole seeds of hemp, flax, and camelina are potentially highly beneficial to human health.
Evaluation of Microwave Applicator Design on Electromagnetic Field Distribution and Heating Pattern of Cooked Peeled Shrimp
Authors: Siguemoto Érica S. , Gut Jorge A. W. , Dimitrakis Georgios , Curet Sebastien , Boillereaux Lionel
Published: August 2021

Non-uniform temperature distribution within solid food is a major problem associated with microwave heating, which limits industrial applications. Therefore, an experimentally validated 3D model was proposed to study the effect of microwave applicator geometry on the electromagnetic field distribution and heating pattern of shrimp under different processing conditions. Simulation results were compared with physical experiments, in which a cooked peeled shrimp sample was heated using two different laboratory-scale microwave applicators (rectangular and cylindrical cavities). For the rectangular applicator, the temperature distribution within the shrimp, when examined in cross-section, was more homogeneous compared to that of the cylindrical applicator. The results showed the influence of the complex shape of the food on the temperature distribution during microwave heating, as well as of process parameters (input power and geometry cavity). Moreover, this modelling method could provide a better understanding of the microwave heating process and assist manufacturing companies to evaluate a suitable microwave applicator according to their specific purpose.
Application of Raw and Defatted by Supercritical CO2 Hemp Seed Press-Cake and Sweet Grass Antioxidant Extract in Pork Burger Patties
Authors: Kerner Kristi , Jõudu Ivi , Tänavots Alo , Venskutonis Petras Rimantas
Published: August 2021

There is an increasing tendency toward the application of plant origin ingredients in meat products. This study evaluates the physicochemical properties and oxidative stability of pork burger patties produced with the addition of dried raw and defatted by supercritical CO2 extraction hemp seed press-cake as protein-rich ingredients (1.5–2.0%) and sweet grass ethanolic extract (0.5%) as a strong natural antioxidant. The main aim of using such a combination was to assess the possibility of mitigating the negative effects of hemp seed press-cake, containing approx. 10% of highly unsaturated oil, on the oxidation of meat products. The patties were compared with the control sample (without additives) during storage on days 0, 4, 8, 15, and 21 at 4 °C in modified atmosphere conditions. Plant ingredients reduced the lightness of pork patties, while their effects on other physicochemical characteristics were insignificant. The patties with fully defatted hemp seed flour showed the lowest grilling losses. Based on the measurement of thiobarbituric acid reactive substances, raw hemp seed press-cake increased the oxidative rate of pork patties; however, remarkably, the addition of sweet grass extract completely inhibited oxidation during the whole period of storage. The sensory characteristics of the products were acceptable; however, the patties with sweet grass extract received lower evaluation scores.
Association of Exosomal miR-210 with Signaling Pathways Implicated in Lung Cancer
Authors: Chen Qiaoyi , Xie Xiaoge
Published: August 2021

MicroRNA is a class of non-coding RNA involved in post-transcriptional gene regulation. Aberrant expression of miRNAs is well-documented in molecular cancer biology. Extensive research has shown that miR-210 is implicated in the progression of multiple cancers including that of the lung, bladder, colon, and renal cell carcinoma. In recent years, exosomes have been evidenced to facilitate cell–cell communication and signaling through packaging and transporting active biomolecules such as miRNAs and thereby modify the cellular microenvironment favorable for lung cancers. MiRNAs encapsulated inside the lipid bilayer of exosomes are stabilized and transmitted to target cells to exert alterations in the epigenetic landscape. The currently available literature indicates that exosomal miR-210 is involved in the regulation of various lung cancer-related signaling molecules and pathways, including STAT3, TIMP-1, KRAS/BACH2/GATA-3/RIP3, and PI3K/AKT. Here, we highlight major findings and progress on the roles of exosomal miR-210 in lung cancer.
Prospects in Connecting Genetic Variation to Variation in Fertility in Male Bees
Authors: Slater Garett P. , Smith Nicholas M. A. , Harpur Brock A.
Published: August 2021

Bees are economically and ecologically important pollinating species. Managed and native bee species face increasing pressures from human-created stressors such as habitat loss, pesticide use, and introduced pathogens. There has been increasing attention towards how each of these factors impacts fertility, especially sperm production and maintenance in males. Here, we turn our attention towards another important factor impacting phenotypic variation: genetics. Using honey bees as a model, we explore the current understanding of how genetic variation within and between populations contributes to variation in sperm production, sperm maintenance, and insemination success among males. We conclude with perspectives and future directions in the study of male fertility in honey bees and non-Apis pollinators more broadly, which still remain largely understudied.
Factors Associated with Depressive Symptoms in Korean Adults with Diabetes Mellitus: A Cross-Sectional Study
Authors: Jeong Mihyun
Published: August 2021

Depressive symptoms in adults with diabetes are influenced by sociodemographic status, health-related behaviors, and comorbid diseases. This study aimed to examine the factors related to depressive symptoms in Korean adults with diabetes, using data from the Korea National Health and Nutrition Examination Surveys for 2014, 2016, and 2018. A total of 1529 Korean adults with diabetes were selected as subjects for the analysis. The age group of the participants was 19–80 years, with a mean age of 63.34 ± 0.68 years. The depressive symptoms and severity were assessed using the Korean version of the Patient Health Questionnaire-9. Descriptive statistics, chi-squared tests, and univariate and multivariable logistic regression analyses were used by applying a complex sample analysis method. The findings showed that 9.6% of Korean adults with diabetes exhibited moderate to severe depressive symptoms, which gradually decreased during 2014–2018. The most significant independent factors of depressive symptoms were living without a spouse, unemployment, low household income, fair or poor subjective health conditions, high perceived stress, a diabetes duration of over 20 years, and stroke. In females, living without a spouse, low household income, poor subjective health condition, high perceived stress, stroke, and coronary heart disease were significantly associated with depressive symptoms. In males, living without a spouse, unemployment, poor subjective health condition, high perceived stress, and hypertension were significantly associated with depressive symptoms. These findings highlight the importance of regular screening for depressive symptoms in patients with diabetes as the prevalence of depressive symptoms in people with diabetes may be higher than those in the general population. Future studies should also examine the development and effectiveness of psychosocial intervention programs to decrease depressive symptoms in patients with diabetes, considering cost-effective and time-saving approaches.
Incorrect Facemask-Wearing Detection Using Convolutional Neural Networks with Transfer Learning
Authors: Tomás Jesús , Rego Albert , Viciano-Tudela Sandra , Lloret Jaime
Published: August 2021

The COVID-19 pandemic has been a worldwide catastrophe. Its impact, not only economically, but also socially and in terms of human lives, was unexpected. Each of the many mechanisms to fight the contagiousness of the illness has been proven to be extremely important. One of the most important mechanisms is the use of facemasks. However, the wearing the facemasks incorrectly makes this prevention method useless. Artificial Intelligence (AI) and especially facial recognition techniques can be used to detect misuses and reduce virus transmission, especially indoors. In this paper, we present an intelligent method to automatically detect when facemasks are being worn incorrectly in real-time scenarios. Our proposal uses Convolutional Neural Networks (CNN) with transfer learning to detect not only if a mask is used or not, but also other errors that are usually not taken into account but that may contribute to the virus spreading. The main problem that we have detected is that there is currently no training set for this task. It is for this reason that we have requested the participation of citizens by taking different selfies through an app and placing the mask in different positions. Thus, we have been able to solve this problem. The results show that the accuracy achieved with transfer learning slightly improves the accuracy achieved with convolutional neural networks. Finally, we have also developed an Android-app demo that validates the proposal in real scenarios.
Overview of Multi-Modal Brain Tumor MR Image Segmentation
Authors: Zhang Wenyin , Wu Yong , Yang Bo , Hu Shunbo , Wu Liang , Dhelim Sahraoui
Published: August 2021

The precise segmentation of brain tumor images is a vital step towards accurate diagnosis and effective treatment of brain tumors. Magnetic Resonance Imaging (MRI) can generate brain images without tissue damage or skull artifacts, providing important discriminant information for clinicians in the study of brain tumors and other brain diseases. In this paper, we survey the field of brain tumor MRI images segmentation. Firstly, we present the commonly used databases. Then, we summarize multi-modal brain tumor MRI image segmentation methods, which are divided into three categories: conventional segmentation methods, segmentation methods based on classical machine learning methods, and segmentation methods based on deep learning methods. The principles, structures, advantages and disadvantages of typical algorithms in each method are summarized. Finally, we analyze the challenges, and suggest a prospect for future development trends.
A Fast and Effective System for Detection of Neonatal Jaundice with a Dynamic Threshold White Balance Algorithm
Authors: Hsu Wei-Yen , Cheng Han-Chang
Published: August 2021

Neonatal jaundice is caused by high levels of bilirubin in the body, which most commonly appears within three days of birth among newborns. Neonatal jaundice detection systems can take pictures in different places and upload them to the system for judgment. However, the white balance problem of the images is often encountered in these detection systems. The color shift images induced by different light haloes will result in the system causing errors in judging the images. The true color of images is very important information when the detection system judges the jaundice value. At present, most systems adopt specific assumption methods and rely on color charts to adjust images. In this study, we propose a novel white balance method with dynamic threshold to screen appropriate feature factors at different color temperatures iteratively and make the adjustment results of different images close to the same. The experimental results indicate that the proposed method achieves superior results in comparison with several traditional approaches.
The San Saturnino Basilica (Cagliari, Italy): An Up-Close Investigation about the Archaeological Stratigraphy of Mortars from the Roman to the Middle Ages
Authors: Sitzia Fabio
Published: August 2021

The manufacturing technology of historical mortars from the Roman to Medieval period apparently has not undergone evolutions. As reported in the literature, a quality decrease in the raw material occurred after the fall of the Roman Empire. During the Roman Age, the mortars presented the requirements of long durability due to hydraulic characteristics, and in later times, the production has only partially maintained the ancient requirements. To focus on the different production technologies between Roman and Medieval mortar, this research presents the case study of San Saturnino Basilica (Italy), where an archaeological mortar stratigraphy from Roman to Middle Ages is well preserved. An archaeometric characterization was performed to compare the mortars of the Roman period with the mortars of the Medieval period collected from the case-study monument. This comparison was carried out by measuring some physical-mechanical, mineralogical, petrographic and thermal features that give more information about the durability and resistance to mechanical solicitations and weathering. After the characterizations, contrary to what is reported in the bibliography, a better quality of Medieval materials than Roman ones is pointed out. This has been highlighted by higher hydraulicity, mechanical performance, and a more appropriated particle-size distribution of aggregates.
Mirrored Social Representations of Canadian Caseworkers with Migratory Paths Intervening with Refugees in the Host Country
Authors: Negura Lilian , Buhay Corinna , de Rosa Annamaria Silvana
Published: August 2021

In 2015, the resettlement of 25,000 Syrian refugees in Canada placed a strain on social services. Caseworkers employed in these agencies often come from similar migratory trajectories to those of the refugees. This experiential proximity requires an understanding of the subjective perspectives that caseworkers with migratory paths have of refugees in the context of their professional practice. We analyzed fifteen individual interviews with Canadian caseworkers and conducted field observations of resettlement activities in the Ottawa-Gatineau region using inductive reasoning inspired by grounded theory. Adopting a sociogenetic approach to social representation theory, this qualitative study illustrates how the social representation of refugees among foreign-born caseworkers is highly informed by their migratory past experience, as well as by the social identity and social context from which that representation was socio-generated. Our analysis reveals the mirror effect of the caseworkers as a fruitful concept for understanding the identity-otherness dynamics in the encounter between the distant other (refugee) and the self.
The Impact of Urban Sprawl on Environmental Pollution: Empirical Analysis from Large and Medium-Sized Cities of China
Authors: Zhang Huan
Published: August 2021

This research sought to uncover the environmental effects that urban sprawl brings about by using panel data of 35 large and medium-sized cities over the timespan 1998–2019. A nonlinear relationship between urban sprawl and environmental pollution might exist and be shown by constructing the conceptual mathematical model. The panel unit root test indicated that there exists a long-run equilibrium relationship between urban sprawl and environmental pollution. Regression results showed that there is a U-shaped curve relationship between the two core variables for the full sample. The impacts of urban sprawl on environmental conditions have regional heterogeneity. A significant U-shaped curve was discovered for eastern and western regions, while an insignificant inverted U-shaped curve was discovered for the central region. Furthermore, we found a significant U-shaped curve for cities with high and low degree of sprawl. By contrast, we found a significant inverted U-shaped curve for cities with a middle degree of sprawl, which indicated that moderate urban sprawl is beneficial for environmental conditions. We additionally discovered a significant U-shaped curve and inverted U-shaped curve for cities with low and middle pollution, respectively. Fixed-asset investments and foreign direct investments exacerbate urban environmental pollution. Market freedom and industrial structure adjustment are conducive to pollution reduction. The conclusions were consistent when we subdivided the sample by time periods and substituted with a systematic GMM (Generalized Method of Moments) estimation method in the robustness check. Policy recommendations were put forward according to the research findings.
Colorectal Cancer Risk in Patients with Hemorrhoids: A 10-Year Population-Based Retrospective Cohort Study
Authors: Wu En-Bo , Sung Fung-Chang , Lin Cheng-Li , Wu Kuen-Lin , Chen Kuen-Bao
Published: August 2021

Colorectal cancer (CRC) is a common disease and one of the leading causes of cancer deaths worldwide. This retrospective cohort study evaluated the risk of developing CRC in people with hemorrhoids. Using Taiwan’s National Health Insurance Research Database, we established three sets of retrospective study cohorts with and without hemorrhoids. The first set of cohorts were matched by sex and age, the second set of cohorts were matched by propensity score without including colonoscopies, and the third set of cohorts were matched by propensity score with colonoscopies, colorectal adenomas, and appendectomies included. In the second set of cohorts, 36,864 persons with hemorrhoids that were diagnosed from 2000 to 2010 and a comparison cohort, with the same size and matched by propensity score, were established and followed up to the end of 2011 to assess the incidence and Cox proportional regression-measured hazard ratio (HR) of CRC. The overall incidence rate of CRC was 2.39 times greater in the hemorrhoid cohort than it was in the comparison cohort (1.29 vs. 0.54 per 1000 person-years), with a multivariable model measured adjusted HR of 2.18 (95% CI = 1.78–2.67) after controlling for sex, age, and comorbidity. Further analysis on the CRC incidence rates among colorectal sites revealed higher incidence rates at the rectum and sigmoid than at other sites, with adjusted HRs 2.20 (95% CI = 1.48–3.28) and 1.79 (95% CI = 1.06–3.02), respectively. The overall incidence rates of both cohorts were similar in the first and second sets of cohorts, whereas the rate was lower in the third set of hemorrhoid cohorts than in the respective comparison cohorts, probably because of overmatching. Our findings suggest that patients with hemorrhoids were at an elevated risk of developing CRC. Colonoscopy may be strongly suggested for identifying CRC among those with hemorrhoids, especially if they have received a positive fecal occult blood test result.
Photocatalytic Treatments for Personal Protective Equipment: Experimental Microbiological Investigations and Perspectives for the Enhancement of Antimicrobial Activity by Micrometric TiO2
Authors: Margarucci Lory Marika , Gianfranceschi Gianluca , Romano Spica Vincenzo , D’Ermo Giuseppe , Refi Cristiano , Podico Maurizio , Vitali Matteo , Romano Ferdinando , Valeriani Federica
Published: August 2021

The COVID-19 pandemic has led to countries enforcing the use of facial masks to prevent contagion. However, acquisition, reuse, and disposal of personal protective equipment (PPE) has generated problems, in regard to the safety of individuals and environmental sustainability. Effective strategies to reprocess and disinfect PPE are needed to improve the efficacy and durability of this equipment and to reduce waste load. Thus, the addition of photocatalytic materials to these materials, combined with light exposure at specific wavelengths, may represent promising solutions. To this aim, we prepared a series of masks by depositing micrometer-sized TiO2 on the external surfaces; the masks were then contaminated with droplets of bacteria suspensions and the coatings were activated by light radiation at different wavelengths. A significant reduction in the microbial load (over 90%, p < 0.01) was observed using both Gram negative (E. coli) and Gram positive (S. aureus) bacteria within 15 min of irradiation, with UV or visible light, including sunlight or artificial sources. Our results support the need for further investigations on self-disinfecting masks and other disposable PPE, which could positively impact (i) the safety of operators/workers, and (ii) environmental sustainability in different occupational or recreational settings.
T. gondii Infection in Urban and Rural Areas in the Amazon: Where Is the Risk for Toxoplasmosis?
Authors: Morais Rafaela dos Anjos Pinheiro Bogoevich , Carmo Ediclei Lima do , Costa Wanda Silva , Marinho Rodrigo Rodrigues , Póvoa Marinete Marins
Published: August 2021

Toxoplasmosis, an infection caused by Toxoplasma gondii, which is found worldwide, can affect human and animal health in different ways. This study aimed to estimate the infection prevalence in humans and to determine risk factors related to urban and rural areas in a municipality in the Brazilian Amazon where an outbreak had been registered. Blood samples for serological analysis were obtained, and interviews were performed to fill out an epidemiological questionnaire. A total of 1140 individuals were included, of which 70.6% (804/1140; 95% CI: 67.9–73.2%) were positive for IgG anti-T. gondii antibodies. In rural areas, the prevalence was 62.6% (95% CI: 58.9–66.3%), while in urban areas, it was 81.9% (95% CI: 78.4–85.4%). The risk of becoming infected in urban areas was 2.7 times higher (95% CI = 2.0–3.6%) than that in rural areas. When comparing the prevalence in the age group from 1 to 10 years in both areas, the rate was 28.6% (42/147; 95% CI: 21.3–35.9%) for rural areas and 69.4% (61/88; CI 95%: 59.7–79.0%) for urban areas. Therefore, it is concluded that parasite exposure starts in the first years of life in urban areas and that disordered urban area expansion may cause an increase in exposure to the different strains of T. gondii present in the Amazon.
Role-Based Cyberbullying Situations: Cybervictims, Cyberaggressors and Cyberbystanders
Authors: González-Calatayud Víctor , Espinosa María Paz Prendes
Published: August 2021

The use of information and communication technology (ICT) has led to new risks, and among them is cyberbullying. It is important to be aware of the prevalence of cyberbullying in order to design intervention plans based on real contexts. Studies, however, vary widely in the data they report. These discrepancies may be due to differences in measurement. The main aim of our study, conducted in the Region of Murcia (Spain), was to ascertain the prevalence of cyberbullying in the three roles involved: victim, perpetrator and bystander. A descriptive, correlational and quantitative study was conducted using a “Cyberbullying: peer harassment screening”questionnaire to collect data. The representative sample comprised 950 students aged between 11 and 18 years (M = 13.93, SD = 1.35). The data showed that 72.1% of the participants had been involved in one or more cyberbullying situation (as victim, aggressor and/or bystander) in the previous year. Specifically, 49.3% had been cybervictims, 23.3% cyberaggressors and 62.3% cyberbystanders. The study provides detailed information about the prevalence of cyberbullying in the Region of Murcia and enables comparisons of the three roles involved. The data point to the need to promote active prevention and psychoeducational intervention strategies.
Erratum: Kadhim, I.; Abed, F.M. The Potential of LiDAR and UAV-Photogrammetric Data Analysis to Interpret Archaeological Sites: A Case Study of Chun Castle in South-West England. ISPRS Int. J. Geo-Inf. 2021, 10, 41
Authors: Kadhim Israa , Abed Fanar M.
Published: August 2021

The authors would like to make the following corrections to the published paper [...]
Evaluation of the Binding Kinetics of RHEB with mTORC1 by In-Cell and In Vitro Assays
Authors: Shams Raef , Ito Yoshihiro , Miyatake Hideyuki
Published: August 2021

The mammalian/mechanistic target of rapamycin complex 1 (mTORC1) is activated by the small G-protein, Ras homolog enriched in brain (RHEB–GTPase). On lysosome, RHEB activates mTORC1 by binding the domains of N-heat, M-heat, and the focal adhesion targeting (FAT) domain, which allosterically regulates ATP binding in the active site for further phosphorylation. The crucial role of RHEB in regulating growth and survival through mTORC1 makes it a targetable site for anti-cancer therapeutics. However, the binding kinetics of RHEB to mTORC1 is still unknown at the molecular level. Therefore, we studied the kinetics by in vitro and in-cell protein–protein interaction (PPI) assays. To this end, we used the split-luciferase system (NanoBiT®) for in-cell studies and prepared proteins for the in vitro measurements. Consequently, we demonstrated that RHEB binds to the whole mTOR both in the presence or absence of GTPγS, with five-fold weaker affinity in the presence of GTPγS. In addition, RHEB bound to the truncated mTOR fragments of N-heat domain (∆N, aa 60–167) or M-heat domain (∆M, aa 967–1023) with the same affinity in the absence of GTP. The reconstructed binding site of RHEB, ∆N-FAT-M, however, bound to RHEB with the same affinity as ∆N-M, indicating that the FAT domain (∆FAT, aa 1240–1360) is dispensable for RHEB binding. Furthermore, RHEB bound to the truncated kinase domain (∆ATP, aa 2148–2300) with higher affinity than to ∆N-FAT-M. In conclusion, RHEB engages two different binding sites of mTOR, ∆N-FAT-M and ∆ATP, with higher affinity for ∆ATP, which likely regulates the kinase activity of mTOR through multiple different biding modes.
Sperm Lipid Markers of Male Fertility in Mammals
Authors: Shan Shuwen , Xu Fangzheng , Hirschfeld Marc , Brenig Bertram
Published: August 2021

Sperm plasma membrane lipids are essential for the function and integrity of mammalian spermatozoa. Various lipid types are involved in each key step within the fertilization process in their own yet coordinated way. The balance between lipid metabolism is tightly regulated to ensure physiological cellular processes, especially referring to crucial steps such as sperm motility, capacitation, acrosome reaction or fusion. At the same time, it has been shown that male reproductive function depends on the homeostasis of sperm lipids. Here, we review the effects of phospholipid, neutral lipid and glycolipid homeostasis on sperm fertilization function and male fertility in mammals.
Sex Differences in Otolaryngology: Focus on the Emerging Role of Estrogens in Inflammatory and Pro-Resolving Responses
Authors: Luo Sheng-Dean , Chiu Tai-Jan , Chen Wei-Chih , Wang Ching-Shuen
Published: August 2021

Otolaryngology (also known as ear, nose, and throat (ENT)) diseases can be significantly affected by the level of sex hormones, which indicates that sex differences affect the manifestation, pathophysiology, and outcomes of these diseases. Recently, increasing evidence has suggested that proinflammatory responses in ENT diseases are linked to the level of sex hormones. The sex hormone receptors are present on a wide variety of immune cells; therefore, it is evident that they play crucial roles in regulating the immune system and hence affect the disease progression of ENT diseases. In this review, we focus on how sex hormones, particularly estrogens, regulate ENT diseases, such as chronic rhinosinusitis, vocal fold polyps, thyroid cancer, Sjögren’s syndrome, and head and neck cancers, from the perspectives of inflammatory responses and specialized proresolving mediator-driven resolution. This paper aims to clarify why considering sex differences in the field of basic and medical research on otolaryngology is a key component to successful therapy for both males and females in the future.
The Role of Mitochondria-Derived Peptides in Cardiovascular Diseases and Their Potential as Therapeutic Targets
Authors: Dabravolski Siarhei A. , Nikiforov Nikita G. , Starodubova Antonina V. , Popkova Tatyana V. , Orekhov Alexander N.
Published: August 2021

Mitochondria-derived peptides (MDPs) are small peptides hidden in the mitochondrial DNA, maintaining mitochondrial function and protecting cells under different stresses. Currently, three types of MDPs have been identified: Humanin, MOTS-c and SHLP1-6. MDPs have demonstrated anti-apoptotic and anti-inflammatory activities, reactive oxygen species and oxidative stress-protecting properties both in vitro and in vivo. Recent research suggests that MDPs have a significant cardioprotective role, affecting CVDs (cardiovascular diseases) development and progression. CVDs are the leading cause of death globally; this term combines disorders of the blood vessels and heart. In this review, we focus on the recent progress in understanding the relationships between MDPs and the main cardiovascular risk factors (atherosclerosis, insulin resistance, hyperlipidaemia and ageing). We also will discuss the therapeutic application of MDPs, modified and synthetic MDPs, and their potential as novel biomarkers and therapeutic targets.
The Interplay between Autophagy and NLRP3 Inflammasome in Ischemia/Reperfusion Injury
Authors: Lv Shuangyu , Liu Huiyang , Wang Honggang
Published: August 2021

Ischemia/reperfusion (I/R) injury is characterized by a limited blood supply to organs, followed by the restoration of blood flow and reoxygenation. In addition to ischemia, blood flow recovery can also lead to very harmful injury, especially inflammatory injury. Autophagy refers to the transport of cellular materials to the lysosomes for degradation, leading to the conversion of cellular components and offering energy and macromolecular precursors. It can maintain the balance of synthesis, decomposition and reuse of the intracellular components, and participate in many physiological processes and diseases. Inflammasomes are a kind of protein complex. Under physiological and pathological conditions, as the cellular innate immune signal receptors, inflammasomes sense pathogens to trigger an inflammatory response. TheNLRP3 inflammasome is the most deeply studied inflammasome and is composed of NLRP3, the adaptor apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and pro-caspase-1. Its activation triggers the cleavage of pro-interleukin (IL)-1β and pro-IL-18 mediated by caspase-1 and promotes a further inflammatory process. Studies have shown that autophagy and the NLRP3 inflammasome play an important role in the process of I/R injury, but the relevant mechanisms have not been fully explained, especially how the interaction between autophagy and the NLRP3 inflammasome participates in I/R injury, which remains to be further studied. Therefore, we reviewed the recent studies about the interplay between autophagy and the NLRP3 inflammasome in I/R injury and analyzed the mechanisms to provide the theoretical references for further research in the future.
First Experimental Study of the Influence of Extracellular Vesicles Derived from Multipotent Stromal Cells on Osseointegration of Dental Implants
Authors: Maiborodin Igor , Shevela Aleksandr , Matveeva Vera , Morozov Vitaly , Toder Michael , Krasil’nikov Sergey , Koryakina Alina , Shevela Andrew , Yanushevich Oleg
Published: August 2021

Herein, the aim was to study the state of the bone tissue adjacent to dental implants after the use of extracellular vesicles derived from multipotent stromal cells (MSC EVs) of bone marrow origin in the experiment. In compliance with the rules of asepsis and antiseptics under general intravenous anesthesia with propofol, the screw dental implants were installed in the proximal condyles of the tibia of outbred rabbits without and with preliminary introduction of 19.2 μg MSC EVs into each bone tissue defect. In 3, 7, and 10 days after the operation, the density of bone tissue adjacent to different parts of the implant using an X-ray unit with densitometer was measured. In addition, the histological examinations of the bone site with the hole from the removed device and the soft tissues from the surface of the proximal tibial condyle in the area of intra-bone implants were made. It was found out that 3 days after implantation with the use of MSC EVs, the bone density was statistically significantly higher by 47.2% than after the same implantation, but without the injection of MSC EVs. It is possible that as a result of the immunomodulatory action of MSC EVs, the activity of inflammation decreases, and, respectively, the degree of vasodilation in bones and leukocyte infiltration of the soft tissues are lower, in comparison with the surgery performed in the control group. The bone fragments formed during implantation are mainly consolidated with each other and with the regenerating bone. Day 10 demonstrated that all animals with the use of MSC EVs had almost complete fusion of the screw device with the bone tissue, whereas after the operation without the application of MSC EVs, the heterogeneous histologic pattern was observed: From almost complete osseointegration of the implant to the absolute absence of contact between the foreign body and the new formed bone. Therefore, the use of MSC EVs during the introduction of dental implants into the proximal condyle of the tibia of rabbits contributes to an increase of the bone tissue density near the device after 3 days and to the achievement of consistently successful osseointegration of implants 10 days after the surgery.
Yeast Models and Molecular Mechanisms of Neurodegenerative Diseases
Authors: Kaminska Joanna , Zoladek Teresa
Published: August 2021

Neurodegenerative diseases are a group of age-related diseases and a growing problem in an aging society [...]
Revealing the Influences of Sex Hormones and Sex Differences in Atrial Fibrillation and Vascular Cognitive Impairment
Authors: Chang Ya-Ting , Chen Yung-Lung , Kang Hong-Yo
Published: August 2021

The impacts of sex differences on the biology of various organ systems and the influences of sex hormones on modulating health and disease have become increasingly relevant in clinical and biomedical research. A growing body of evidence has recently suggested fundamental sex differences in cardiovascular and cognitive function, including anatomy, pathophysiology, incidence and age of disease onset, symptoms affecting disease diagnosis, disease severity, progression, and treatment responses and outcomes. Atrial fibrillation (AF) is currently recognized as the most prevalent sustained arrhythmia and might contribute to the pathogenesis and progression of vascular cognitive impairment (VCI), including a range of cognitive deficits, from mild cognitive impairment to dementia. In this review, we describe sex-based differences and sex hormone functions in the physiology of the brain and vasculature and the pathophysiology of disorders therein, with special emphasis on AF and VCI. Deciphering how sex hormones and their receptor signaling (estrogen and androgen receptors) potentially impact on sex differences could help to reveal disease links between AF and VCI and identify therapeutic targets that may lead to potentially novel therapeutic interventions early in the disease course of AF and VCI.
The CellBox-2 Mission to the International Space Station: Thyroid Cancer Cells in Space
Authors: Melnik Daniela , Krüger Marcus , Schulz Herbert , Kopp Sascha , Wehland Markus , Bauer Johann , Baselet Bjorn , Vermeesen Randy , Baatout Sarah , Corydon Thomas J. , Infanger Manfred , Grimm Daniela
Published: August 2021

A spaceflight to the International Space Station (ISS) is a dream of many researchers. We had the chance to investigate the effect of real microgravity (CellBox-2 Space mission) on the transcriptome and proteome of FTC-133 human follicular thyroid cancer cells (TCC). The cells had been sent to the ISS by a Falcon 9 rocket of SpaceX CRS-13 from Cape Canaveral (United States) and cultured in six automated hardware units on the ISS before they were fixed and returned to Earth. Multicellular spheroids (MCS) were detectable in all spaceflight hardware units. The VCL, PXN, ITGB1, RELA, ERK1 and ERK2 mRNA levels were significantly downregulated after 5 days in space in adherently growing cells (AD) and MCS compared with ground controls (1g), whereas the MIK67 and SRC mRNA levels were both suppressed in MCS. By contrast, the ICAM1, COL1A1 and IL6 mRNA levels were significantly upregulated in AD cells compared with 1g and MCS. The protein secretion measured by multianalyte profiling technology and enzyme-linked immunosorbent assay (AngiogenesisMAP®, extracellular matrix proteins) was not significantly altered, with the exception of elevated angiopoietin 2. TCC in space formed MCS, and the response to microgravity was mainly anti-proliferative. We identified ERK/RELA as a major microgravity regulatory pathway.
Naturalistic Stress Hormone Levels Drive Cumulative Epigenomic Changes along the Cellular Lifespan
Authors: Zannas Anthony
Published: August 2021

Environmental stress is ubiquitous in modern societies and can exert a profound and cumulative impact on cell function and health phenotypes. This impact is thought to be in large part mediated by the action of glucocorticoid stress hormones, primarily cortisol in humans. While the underlying molecular mechanisms are unclear, epigenetics—the chemical changes that regulate genomic function without altering the genetic code—has emerged as a key link between environmental exposures and phenotypic outcomes. The present study assessed genome-wide DNA (CpG) methylation, one of the key epigenetic mechanisms, at three timepoints during prolonged (51-day) exposure of cultured human fibroblasts to naturalistic cortisol levels, which can be reached in human tissues during in vivo stress. The findings support a spatiotemporal model of profound and widespread stress hormone-driven methylomic changes that emerge at selected CpG sites, are more likely to spread to nearby located CpGs, and quantitatively accrue at open sea, glucocorticoid receptor binding, and chromatin-accessible sites. Taken together, these findings provide novel insights into how prolonged stress may impact the epigenome, with potentially important implications for stress-related phenotypes.
Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency
Authors: Zhang Tianrui , Xiao Zhidan , Liu Chuanliang , Yang Chao , Li Jiayi , Li Hongbo , Gao Caiji , Shen Wenjin
Published: August 2021

Multivesicular body (MVB)-mediated endosomal sorting and macroautophagy are the main pathways mediating the transport of cellular components to the vacuole and are essential for maintaining cellular homeostasis. The interplay of these two pathways remains poorly understood in plants. In this study, we show that FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT), essential for MVB biogenesis and plant growth, can be transported to the vacuole for degradation in response to iron deficiency. The vacuolar transport of ubiquitinated FREE1 protein is mediated by the autophagy pathway. As a consequence, the autophagy deficient mutants, atg5-1 and atg7-2, accumulate more endogenous FREE1 protein and display hypersensitivity to iron deficiency. Furthermore, under iron-deficient growth condition autophagy related genes are upregulated to promote the autophagic degradation of FREE1, thereby possibly relieving the repressive effect of FREE1 on iron absorption. Collectively, our findings demonstrate a unique regulatory mode of protein turnover of the ESCRT machinery through the autophagy pathway to respond to iron deficiency in plants.
NLRP3 Ubiquitination—A New Approach to Target NLRP3 Inflammasome Activation
Authors: Akther Mahbuba , Haque Md Ezazul , Park Jooho , Kang Tae-Bong , Lee Kwang-Ho
Published: August 2021

In response to diverse pathogenic and danger signals, the cytosolic activation of the NLRP3 (NOD-, LRR-, and pyrin domain-containing (3)) inflammasome complex is a critical event in the maturation and release of some inflammatory cytokines in the state of an inflammatory response. After activation of the NLRP3 inflammasome, a series of cellular events occurs, including caspase 1-mediated proteolytic cleavage and maturation of the IL-1β and IL-18, followed by pyroptotic cell death. Therefore, the NLRP3 inflammasome has become a prime target for the resolution of many inflammatory disorders. Since NLRP3 inflammasome activation can be triggered by a wide range of stimuli and the activation process occurs in a complex, it is difficult to target the NLRP3 inflammasome. During the activation process, various post-translational modifications (PTM) of the NLRP3 protein are required to form a complex with other components. The regulation of ubiquitination and deubiquitination of NLRP3 has emerged as a potential therapeutic target for NLRP3 inflammasome-associated inflammatory disorders. In this review, we discuss the ubiquitination and deubiquitination system for NLRP3 inflammasome activation and the inhibitors that can be used as potential therapeutic agents to modulate the activation of the NLRP3 inflammasome.