bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Analysis and Strength Estimation of Fresh Concrete Based on Ultrasonic Wave Propagation and Maturity Using Smart Temperature and PZT Sensors
Authors: Tareen Najeebullah , Kim Junkyeong , Kim Won-Kyu , Park Seunghee
Published: August 2019

Recently, the early-age strength prediction for RC (reinforced concrete) structures has been an important topic in the construction industry, relating to project-time reduction and structural safety. To address this, numerous destructive and NDTs (non-destructive tests) are applied to monitor the early-age strength development of concrete. This study elaborates on the NDT techniques of ultrasonic wave propagation and concrete maturity for the estimation of compressive strength development. The results of these comparative estimation approaches comprise the concrete maturity method, penetration resistance test, and an ultrasonic wave analysis. There is variation of the phase transition in the concrete paste with the changing of boundary limitations of the material in accordance with curing time, so with the formation of phase-transition changes, changes in the velocities of ultrasonic waves occur. As the process of hydration takes place, the maturity method produces a maturity index using the time-feature reflection on the strength-development process of the concrete. Embedded smart temperature sensors (SmartRock) and PZT (piezoelectric) sensors were used for the data acquisition of hydration temperature history and wave propagation. This study suggests a novel relationship between wave propagation, penetration tests, and hydration temperature, and creates a method that relies on the responses of resonant frequency changes with the change of boundary conditions caused by the strength-gain of the concrete specimen. Calculating the changes of these features provides a pattern for estimating concrete strength. The results for the specimens were validated by comparing the strength results with the penetration resistance test by a universal testing machine (UTM). An algorithm used to relate the concrete maturity and ultrasonic wave propagation to the concrete compressive strength. This study leads to a method of acquiring data for forecasting in-situ early-age strength of concrete, used for secure construction of concrete structures, that is fast, cost effective, and comprehensive for SHM (structural health monitoring).
A Large Measurable Range Capacitance-to-Digital Converter for Smart Humidity Sensors
Authors: Wei Rongshan , Lin Weiwen , Xiao Xiaoxia , Chen Qunchao , Li Fanyang
Published: August 2019

This study aims to propose a capacitance-to-digital converter (CDC) based on a third-order cascade of integrators with a feed-forward (CIFF) incremental sigma-delta modulator for smart humidity sensor application. Disguised zoom-in technology was proposed to enlarge the measurable range of the CDC. The input range of the CDC was 0–388 pF. The proposed CDC was realized using 0.18 μm complementary metal-oxide-semiconductor technology. Results show that the CDC performs a 13-bit capacitance-to-digital conversion in 0.8 ms. The analog system consumes 169.7 μA from a 1.8 V supply, which corresponds to a figure of merit (FOM) of 3.0 nJ/step. The proposed CDC was combined with a HS1101 humidity sensor to demonstrate its incorporation in an overall system design. The resolution was 0.7% relative humidity (RH) over a range of 30%–90% RH.
Direct Numerical Simulation of Seawater Desalination Based on Ion Concentration Polarization
Authors: Li Jie , Chen Dilin , Ye Jian , Zhang Lai , Zhou Teng , Zhou Yi
Published: August 2019

The problem of water shortage needs to be solved urgently. The membrane-embedded microchannel structure based on the ion concentration polarization (ICP) desalination effect is a potential portable desalination device with low energy consumption and high efficiency. The electroosmotic flow in the microchannel of the cation exchange membrane and the desalination effect of the system are numerically analyzed. The results show that when the horizontal electric field intensity is 2 kV/m and the transmembrane voltage is 400 mV, the desalting efficiency reaches 97.3%. When the electric field strength increases to 20 kV/m, the desalination efficiency is reduced by 2%. In terms of fluid motion, under the action of the transmembrane voltage, two reverse eddy currents are formed on the surface of the membrane due to the opposite electric field and pressure difference on both sides of the membrane, forming a pumping effect. The electromotive force in the channel exhibits significant pressure-flow characteristics with a slip boundary at a speed approximately six times that of a non-membrane microchannel.
Fabrication of Simultaneously Implementing “Wired Face-Up and Face-Down Ultrathin Piezoresistive Si Chips” on a Film Substrate by Screen-Offset Printing
Authors: Takei Yusuke , Nomura Ken-ichi , Horii Yoshinori , Zymelka Daniel , Ushijima Hirobumi , Kobayashi Takeshi
Published: August 2019

We realized the implementation of an ultrathin piezoresistive Si chip and stretchable printed wires on a flexible film substrate using simple screen-offset printing technology. This process does not require a special MEMS fabrication equipment and is applicable to face-up chips where electrodes are formed on the top surface of the chip, as well as to face-down chips where electrodes are formed on the bottom surface of the chip. This fabrication process is quite useful in the field of flexible hybrid electronics (FHE) as a method for mounting and wiring electronic components on a flexible substrate. In this study, we confirmed that face-up and face-down chips could be mounted on polyimide film tape. Furthermore, it was confirmed that the two types of chips could be simultaneously mounted even if they exist on the same substrate. Five-μm-thick piezoresistive Si chips were transferred and wired on a polyimide film tape using screen-offset printing, and a band-plaster type blood pulse sensor was fabricated. Moreover, we successfully demonstrated that the blood pulse could be measured with neck, inner elbow, wrist, and ankle.
Multimodal Nonlinear Microscopy for Therapy Monitoring of Cold Atmospheric Plasma Treatment
Authors: Meyer Tobias , Bae Hyeonsoo , Hasse Sybille , Winter Jörn , Woedtke Thomas von , Schmitt Michael , Weltmann Klaus-Dieter , Popp Juergen
Published: August 2019

Here we report on a non-linear spectroscopic method for visualization of cold atmospheric plasma (CAP)-induced changes in tissue for reaching a new quality level of CAP application in medicine via online monitoring of wound or cancer treatment. A combination of coherent anti-Stokes Raman scattering (CARS), two-photon fluorescence lifetime imaging (2P-FLIM) and second harmonic generation (SHG) microscopy has been used for non-invasive and label-free detection of CAP-induced changes on human skin and mucosa samples. By correlation with histochemical staining, the observed local increase in fluorescence could be assigned to melanin. CARS and SHG prove the integrity of the tissue structure, visualize tissue morphology and composition. The influence of plasma effects by variation of plasma parameters e.g., duration of treatment, gas composition and plasma source has been evaluated. Overall quantitative spectroscopic markers could be identified for a direct monitoring of CAP-treated tissue areas, which is very important for translating CAPs into clinical routine.
Estimates of scCO2 Storage and Sealing Capacity of the Janggi Basin in Korea Based on Laboratory Scale Experiments
Authors: Park Jinyoung , Yang Minjune , Kim Seyoon , Lee Minhee , Wang Sookyun
Published: August 2019

Laboratory experiments were performed to measure the supercritical CO2 (scCO2) storage ratio (%) of conglomerate and sandstone in the Janggi Basin, which are classified as rock in Korea that are available for CO2 storage. The scCO2 storage capacity was evaluated by direct measurement of the amount of scCO2 replacing the pore water in each reservoir rock core. The scCO2 sealing capacity of the cap rock (i.e., tuff and mudstone) was also compared by measuring the scCO2 capillary entry pressure (Δp) into the rock core. The measured average scCO2 storage ratio of the conglomerate and the sandstone were 30.7% and 13.1%, respectively, suggesting that the scCO2 storage capacity was greater than 360,000 metric tons. The scCO2 capillary entry pressure for the tuff ranged from 15 to 20 bar and for the mudstone it was higher than 150 bar, suggesting that the mudstone layers had enough sealing capacity from the aspect of hydromechanics. From XRF analyses, before and after 90 d of the scCO2-water-cap rock reaction, the mudstone and the tuff were investigated to assure their geochemical stability as the cap rock. From the study, the Janggi Basin was considered an optimal CO2 storage site based on both its high scCO2 storage ratio and high capillary entry pressure.
Crepidatumines C and D, Two New Indolizidine Alkaloids from Dendrobium crepidatum Lindl. ex Paxt.
Authors: Xu Xiaolin , Li Zesheng , Yang Runmei , Zhou Houguang , Bai Yanbin , Yu Meng , Ding Gang , Li Biao
Published: August 2019

Two new indolizidine alkaloids, crepidatumines C (1) and D (2), together with crepidine (3), isocrepidamine (4), and crepidamine (5) were isolated from the Dendrobium crepidatum Lindl. ex Paxt. X-ray diffraction experiments established the absolute configurations of known compounds 3 and 4. The planar structures and relative configurations of new compounds 1 and 2 were elucidated by extensive spectra analysis including HR-ESI-MS, NMR (1H, 13C, 1H-1H COSY, HSQC, HMBC, and NOESY spectra), and the absolute configurations of 1 and 2 were suggested on the basis of possible biosynthetic pathways. The biological results confirmed that isocrepidamine (4) displayed a potent hypoglycemic effect in vitro without cytotoxicity.
Rapid Screening of Forskolin-Type Diterpenoids of Blumea aromatica DC Using Ultra-High-Performance Liquid Chromatography Tandem Quadrupole Time-Of-Flight Mass Spectrometry Based on the Mass Defect Filtering Approach
Authors: He Lili , Zhang Zhifeng , Yao Caiyun , Miao Jianhua , Yan Bingxiong , Wu Lingling , Pan Limei , Song Zhijun , Wei Shugen
Published: August 2019

The discovery of new active compounds of natural products tends to be increasingly more challenging due to chemical complexity and unpredictable matrices. Forskolin is an active natural labdane-type diterpenoid ingredient widely used worldwide for the treatment of glaucoma, heart failure, hypertension, diabetes, and asthma, and is expected to be a promising anticancer, anti-inflammation, and anti-HIV agent. In recent years, demand for forskolin in the medicine market has increased dramatically. However, natural forskolin originates exclusively from traditional Indian herb medicine Coleus forskohlii (Willd.) Briq. In a previous study, we isolated a series of diterpenoids including an 8,13-epoxy-14ene labdane carbon skeleton from Blumea aromatica DC. In order to identify alternative plant resources, a novel and effective strategy was proposed for the screening of potential forskolin-type diterpenoids (FSKD) compounds obtained from B. aromatica, using the mass defect filtering (MDF) strategy via ultra-high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) approach. Within a narrow, well-defined mass defect range, the strategy developed could significantly improve the detection efficiency of selected FSKD compounds by filtering out certain major or moderate interference compounds. Additionally, the MS/MS cleavage behavior and the characteristic diagnostic ions of the FSKD compounds were proposed to be used in aiding structural identification of the filtration compounds. As a result, a total of 38 FSKD of B. aromatica were filtered out and tentatively identified. To the best of our knowledge, it was the first time that these forskolin-type diterpenoids were identified in B. aromatica, which significantly expands our understanding of the chemical constituents of Blumea species, and allows B. aromatica to be used as a potential alternative plant resource that contains these forskolin-type active compounds. The strategy proposed was proven efficient and reliable for the discovery of novel compounds of herbal extracts.
Rice Husk Hydrolytic Lignin Transformation in Carbonization Process
Authors: Yefremova Svetlana , Zharmenov Abdurassul , Sukharnikov Yurij , Bunchuk Lara , Kablanbekov Askhat , Anarbekov Kuanish , Kulik Tetiana , Nikolaichuk Alina , Palianytsia Borys
Published: August 2019

Lignin processing products have an extensive using range. Because products properties depend on lignin precursor quality it was interesting to study lignin isolated from rice husk being a large tonnage waste of rice production and its structural transformations during carbonization. Lignin isolated by the thermal hydrolysis method with H2SO4 1 wt % solution and its carbonized products prepared under different carbonization conditions were characterized using elemental analysis, IR, TPD-MS, XRD, TEM, and EPR. It was shown lignin degradation takes place over the wide (220–520 °C) temperature range. Silica presenting in lignin affects the thermal destruction of this polymer. Due to the strong chemical bond with phenolic hydroxylic group it decreases an evaporation of volatile compounds and as a result increases the temperature range of the lignin degradation. Rice husk hydrolytic lignin transformations during carbonization occur with generation of free radicals. Their concentration is decreased after condensation of aromatic rings with carbon polycycles formation, i.e., the graphite-like structure. Quantity and X-ray diffraction characteristics of the graphite-like phase depend on carbonization conditions. Morphology of the lignin-based carbonized products is represented by carbon fibers, carbon and silica nanoparticles, and together with another structure characteristics provides prospective performance properties of lignin-based end products.
Development and Validation of a Simple LC-MS Method for the Quantification of Oxytocin in Dog Saliva
Authors: Wang Lei , Marti Dakota W. , Anderson Rachel E.
Published: August 2019

Oxytocin (OT) is a mammalian neuropeptide with various functions in regulating birth, lactation, parenting, and social recognition. The study of OT became of increasing interest for the petcare industry due to its role in animal behavior and socialization. In the present study, a simple, sensitive, and accurate liquid chromatography-mass spectrometry (LC-MS) method for quantifying OT in dog saliva was developed and validated. OT and its deuterated internal standard (OT-d5) were detected with multiple reaction monitoring (MRM) in a positive ion mode using an AB Sciex 6500+ QTRAP mass spectrometer. Chromatographic separation was achieved by using an ACE Excel C18 column and a gradient elution at a flow rate of 0.5 mL/min over a 5 min run. The mobile phases consisted of 0.1% (v/v) acetic acid in water and 0.1% (v/v) acetic acid in acetonitrile. After development and optimization, the performance of the method was validated to prove its reliability. Calibration curves were linear over the range of 50–20,000 pg/mL and recovery of OT was above 87.8%. The validated method was successfully applied to evaluate OT concentrations in multiple batches of dog saliva samples.
The Entrapment of Somatostatin in a Lipid Formulation: Retarded Release and Free Radical Reactivity
Authors: Larocca Anna , Toniolo Gianluca , Tortorella Silvia , Krokidis Marios , Menounou Georgia , Di Bella Giuseppe , Chatgilialoglu Chryssostomos , Ferreri Carla
Published: August 2019

The natural peptide somatostatin has hormonal and cytostatic effects exerted by the binding to specific receptors in various tissues. Therapeutic uses are strongly prevented by its very short biological half-life of 1–2 min due to enzymatic hydrolysis, therefore encapsulation methodologies are explored to overcome the need for continuous infusion regimes. Multilamellar liposomes made of natural phosphatidylcholine were used for the incorporation of a mixture of somatostatin and sorbitol dissolved in citrate buffer at pH = 5. Lyophilization and reconstitution of the suspension were carried out, showing the flexibility of this preparation. Full characterization of this suspension was obtained as particle size, encapsulation efficiency and retarded release properties in aqueous medium and human plasma. Liposomal somatostatin incubated at 37 °C in the presence of Fe(II) and (III) salts were used as a biomimetic model of drug-cell membrane interaction, evidencing the free radical processes of peroxidation and isomerization that transform the unsaturated fatty acid moieties of the lipid vesicles. This study offers new insights into a liposomal delivery system and highlights molecular reactivity of sulfur-containing drugs with its carrier or biological membranes for pharmacological applications.
Evaluation of Tryptic Podocin Peptide in Urine Sediment Using LC-MS-MRM Method as a Potential Biomarker of Glomerular Injury in Dogs with Clinical Signs of Renal and Cardiac Disorders
Authors: Szczepankiewicz Barbara , Bąchor Remigiusz , Pasławski Robert , Siwińska Natalia , Pasławska Urszula , Konieczny Andrzej , Szewczuk Zbigniew
Published: August 2019

The early asymptomatic stage of glomerular injury is a diagnostic challenge in the course of renal and extra-renal disease, e.g., heart insufficiency. It was found that podocin, a podocyte-specific protein present in the urine, may serve as a biomarker in the diagnosis of glomerular disease in humans and animals including glomerulonephritis, glomerulosclerosis, amyloidosis, or nephropathy. Therefore, there is a need of development of the sensitive and straightforward method of urinary podocin identification. In this work, we report our extended research under the glomerular injury investigation in dogs by application of clinical examination and LC-MS-MRM method in the identification of canine podocin in urine samples. The LC-MS-MRM method is based on the identification of podocin tryptic peptide with the 218H-AAEILAATPAAVQLR-OH232 sequence. The model peptide was characterized by the highest ionization efficiency of all the proposed model podocin tryptic peptides in a canine urine sediment according to the LC-MS/MS analysis. The obtained results revealed the presence of the model peptide in 40.9% of dogs with MMVD (active glomerular injury secondary to heart disease = cardiorenal syndrome-CRS) and 33.3% dogs with chronic kidney disease. The potential applicability of the developed methodology in the analysis of podocin in canine urine sediments was confirmed.
Synthesis, Structure, and Reactivity of Binaphthyl Supported Dihydro[1,6]diazecines
Authors: Lemmerer Miran , Abraham Michael , Brutiu Bogdan R. , Roller Alexander , Widhalm Michael
Published: August 2019

A short approach to chiral diaza-olefines from protected 2,2′-diamino-1,1′-binaphthyl is presented. Cis- and trans-olefines can be selectively obtained by twofold N-allylation followed by RCM or by bridging a 2,2′-diamino-1,1′-binaphthyl precursor with trans-1,4-dibromo-2-butene. Deprotection afforded cis- and trans-dihydro[1,6]diazecines 1 in 58 and 64% overall yield. The reactivity of the but-2-ene-1,4-diyl fragment was investigated yielding corresponding epoxides, diols, and mono- and dibromo products. In several cases rearrangements and participation of the proximate N-Boc group was observed. In no case could allylic substitution be accomplished. From 13 compounds X-ray structure analyses could be obtained.
Nanoencapsulation Enhances Anticoagulant Activity of Adenosine and Dipeptide IleTrp
Authors: Nguyen Trung Dinh , Nguyen The Ngoc , Nguyen Trang Thuy Thi , Ivanov Igor A. , Nguyen Khoa Cuu , Tran Quyen Ngoc , Hoang Anh Ngoc , Utkin Yuri N.
Published: August 2019

It is well-known that drugs administered into an organism intravenously or through the gastrointestinal tract are degraded by enzymes of the body, reducing their therapeutic effect. One of the ways to decrease this undesirable process is through the inclusion of drugs in nanomaterials. Earlier strong anticoagulant activity was demonstrated for dipeptide IleTrp (IW) and adenosine (Ado). In this work, the effect of inclusion in nanomaterials on the biological activity of IW and Ado was studied. For this purpose, Ado and IW were incorporated into thermosensitive nanogel composed of pluronic P123-grafted heparin. The prepared nanocarrier was characterized by transmission electron microscopy, dynamic light scattering, and ζ-potential. Biological activity was determined by measuring the bleeding time from mouse tail in vivo and the time of clot formation in vitro. It was found that encapsulation of Ado and IW into nanomaterial significantly increased their effects, resulting in an increase in the bleeding time from mouse tail and clot formation time. Thus, inclusion of low molecular weight anticoagulants Ado and IW into nanomaterials may be considered a way to increase their biological activity.
Elaboration and Characterization of Active Films Containing Iron–Montmorillonite Nanocomposites for O2 Scavenging
Authors: Kombaya-Touckia-Linin Erland-Modeste , Gaucel Sébastien , Sougrati Moulay T. , Stievano Lorenzo , Gontard Nathalie , Guillard Valérie
Published: August 2019

Iron particles of sizes between 6 and 20 nm forming aggregates of 57 ± 17 nm were synthesized by chemical reduction of iron precursors on the surface of montmorillonite (MMT). This active MMT-Fe powder was then uniformly distributed in a linear low-density polyethylene (LLDPE) matrix by extrusion at atmospheric conditions, as confirmed by wide-angle X-ray scattering (WAXS), which also detected a partial exfoliation of the nanoclays. Thermogravimetric analysis (TGA) did not detect any significant modification of the degradation temperature between nanocomposites and active nanocomposites. 57Fe Mössbauer spectroscopy evidenced the formation of a majority of iron boride in MMT-Fe as well as in the active film containing it. The LLDPE.Fu15.MMT-Fe3.75 and LLDPE.Fu15.MMT-Fe6.25 films had oxygen-scavenging capacities of 0.031 ± 0.002 and 0.055 ± 0.009 g(O2)/g(Fe), respectively, while the neat powder had an adsorption capacity of 0.122 g(O2)/g(Fe). This result confirms that the fresh film samples were partially oxidized shortly after thermomechanical processing (60% of oxidized species according to Mössbauer spectroscopy). No significant difference in oxygen permeability was observed when MMT-Fe was added. This was related to the relatively small film surface used for measuring the permeability. The reaction–diffusion model proposed here was able to reproduce the observed data of O2 adsorption in an active nanocomposite, which validated the O2 adsorption model previously developed for dried MMT-Fe powder.
Organic–Inorganic Hybrid Nanomaterials
Authors: Ananikov Valentine P.
Published: August 2019

The paramount progress in the field of organic–inorganic hybrid nanomaterials was stimulated by numerous applications in chemistry, physics, life sciences, medicine, and technology. Currently, in the field of hybrid materials, researchers may choose either to mimic complex natural materials or to compete with nature by constructing new artificial materials. The deep mechanistic understanding and structural insight achieved in recent years will guide a new wave in the design of hybrid materials at the atomic and molecular levels.
Association of Low Serum l-Carnitine Levels with Peripheral Arterial Stiffness in Patients Who Undergo Kidney Transplantation
Authors: Lai Yu-Hsien , Lee Ming-Che , Ho Guan-Jin , Liu Chin-Hung , Hsu Bang-Gee
Published: August 2019

l-carnitine is an important co-factor in fatty-acid metabolism, and its deficiency is associated with insulin resistance, which is independently associated with arterial stiffness. This study evaluated the relationship between serum l-carnitine level and peripheral arterial stiffness (PAS) in kidney transplantation (KT). Fasting blood samples were collected from 65 patients who underwent KT. We measured the brachial–ankle pulse wave velocity, and 36 patients (55.4%) had PAS. Patients with PAS had a significantly higher percentage of diabetes (p = 0.001), hypertension (p = 0.033), and metabolic syndrome (p = 0.044); higher waist circumference (p = 0.010), systolic blood pressure (p = 0.002), serum triglyceride level (p = 0.040), insulin level (p = 0.002), and homeostasis model assessment of insulin resistance (p = 0.002); lower high-density lipoprotein cholesterol (p = 0.036) and serum l-carnitine levels (p < 0.001); older age (p = 0.041); and a longer KT duration (p = 0.025) than those without PAS. Statistical analysis revealed an independent association between PAS in KT and KT duration (95% confidence interval (CI): 1.003–1.054, p = 0.029) and serum l-carnitine levels (95% CI: 0.842–0.998, p = 0.044). The area under the receiver operating characteristic curve indicated that the diagnostic power of l-carnitine to predict PAS was 0.789 (95% CI: 0.670–0.881, p < 0.001). Serum-free l-carnitine level is negatively associated with PAS in patients who undergo KT.
Tolerability and Safety of a Nutritional Supplement with Potential as Adjuvant in Colorectal Cancer Therapy: A Randomized Trial in Healthy Volunteers
Authors: Gómez de Cedrón Marta , Laparra José Moises , Loria-Kohen Viviana , Molina Susana , Moreno-Rubio Juan , Montoya Juan Jose , Torres Carlos , Casado Enrique , Reglero Guillermo , Ramírez de Molina Ana
Published: August 2019

Bioactive supplements display relevant therapeutic properties when properly applied according to validated molecular effects. Our previous research efforts established the basis to develop a dietary supplement based on a Rosmarinus officinalis supercritical extract. This was enriched in phenolic diterpenes (RE) with proven properties against signaling pathways involved in colon tumorigenesis, and shark liver oil rich in alkylglycerols (AKG) as a bioactive lipid vehicle to improve RE bioavailability and synergize with the potential therapeutic action of the extract. Herein, we have investigated the tolerability and safety of the supplement and the biological and molecular effects from an immuno-nutritional perspective. Sixty healthy volunteers participated in a six week, double-blind, randomized parallel pilot study with two study arms: RE-AKG capsules (CR) and control capsules (CC). Mean age (±SD) of volunteers was 28.32 (±11.39) and 27.5 (±9.04) for the control and the study groups, respectively. Safety of the CR product consumption was confirmed by analyzing liver profile, vital constants, and oxidation markers (LDLox in blood and isoprostanes and thromboxanes in urine). The following were monitored: (1) the phenotyping of plasmatic leukocytes and the ex vivo response of lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs); (2) expression of genes associated with immune-modulation, inflammation, oxidative stress, lipid metabolism, and tumorigenesis; and (3) the correlation of selected genetic variants (SNPs) with the differential responses among individuals. The lack of adverse effects on liver profile and oxidation markers, together with adequate tolerability and safe immunological adaptations, provide high-quality information for the potential use of CR as co-adjuvant of therapeutic strategies against colorectal cancer.
Probiotic Supplementation is Associated with Increased Antioxidant Capacity and Copper Chelation in C. difficile-Infected Fecal Water
Authors: Gaisawat Mohd Baasir , Iskandar Michèle M. , MacPherson Chad W. , Tompkins Thomas A. , Kubow Stan
Published: August 2019

Probiotic supplementation plays a key role in maintaining intestinal homeostasis due to its ability to modulate gut microbiota. Although their potential as potent antioxidants have previously been explored, their ability to affect the redox status in the gut lumen of healthy subjects or those with gastrointestinal (GI) disorders remains unclear. In our study, we assessed the ability of single strain and multispecies probiotic supplementation to cause a change in the redox status of normal fecal water and in Clostridium (C.) difficile-infected fecal water using a simulated gastrointestinal model. Changes in redox status were assessed by ferric-reducing antioxidant power (FRAP), 2’,2’-diphenyl-1-picrylhydrazyl (DPPH), and iron and copper chelation assays. The findings from our study showed that in normal fecal water, probiotic supplements, apart from Lactobacillus (L.) rhamnosus R0011, showed a significant increase in iron chelation (p < 0.05), which was associated with lower FRAP and copper chelation. In C. difficile-infected fecal water, all probiotic supplements showed a significant increase in FRAP (p < 0.05) and were associated with increased copper chelation. The DPPH assay showed no treatment effect in either fecal water. These findings suggest that C. difficile mediates dysregulation of redox status, which is counteracted by probiotics through ferric-reducing ability and copper chelation.
Unraveling Hepcidin Plasma Protein Binding: Evidence from Peritoneal Equilibration Testing
Authors: Diepeveen Laura E. , Laarakkers Coby M. , Peters Hilde P.E. , van Herwaarden Antonius E. , Groenewoud Hans , IntHout Joanna , Wetzels Jack F. , van Swelm Rachel P.L. , Swinkels Dorine W.
Published: August 2019

Peptide hormone hepcidin regulates systemic iron metabolism and has been described to be partially bound to α2-macroglobulin and albumin in blood. However, the reported degree of hepcidin protein binding varies between <3% and ≈89%. Since protein-binding may influence hormone function and quantification, better insight into the degree of hepcidin protein binding is essential to fully understand the biological behavior of hepcidin and interpretation of its measurement in patients. Here, we used peritoneal dialysis to assess human hepcidin protein binding in a functional human setting for the first time. We measured freely circulating solutes in blood and peritoneal fluid of 14 patients with end-stage renal disease undergoing a peritoneal equilibration test to establish a curve describing the relation between molecular weight and peritoneal clearance. Calculated binding percentages of total cortisol and testosterone confirmed our model. The protein-bound fraction of hepcidin was calculated to be 40% (±23%). We, therefore, conclude that a substantial proportion of hepcidin is freely circulating. Although a large inter-individual variation in hepcidin clearance, besides patient-specific peritoneal transport characteristics, may have affected the accuracy of the determined binding percentage, we describe an important step towards unraveling human hepcidin plasma protein binding in vivo including the caveats that need further research.
Towards the Oral Treatment of Ileo-Colonic Inflammatory Bowel Disease with Infliximab Tablets: Development and Validation of the Production Process
Authors: Gareb Bahez , Posthumus Silke , Beugeling Max , Koopmans Pauline , Touw Daan J. , Dijkstra Gerard , Kosterink Jos G.W. , Frijlink Henderik W.
Published: August 2019

Infliximab (IFX) is an intravenously administered monoclonal antibody antagonizing the effects of tumor necrosis factor-alpha (TNF) systemically and is efficacious in the treatment of inflammatory bowel disease (IBD). However, studies suggest that the anti-inflammatory effects result from local immunomodulation in the inflamed regions. Furthermore, topical inhibition of TNF in IBD ameliorates inflammation. We therefore hypothesized that orally administered IFX targeted to the ileo-colonic region in IBD may be an efficacious new treatment option. This study describes the development and validation of the production process of ileo-colonic-targeted 5 mg IFX tablets (ColoPulse-IFX) intended for the oral treatment of IBD by means of producing three consecutive validation batches (VAL1, VAL2, and VAL3, respectively). UV-VIS spectroscopy, HPLC-SEC analysis (content, fragments, aggregates), fluorescence spectroscopy (tertiary protein structure), and ELISA (potency) showed no noticeable deviations of IFX compounded to ColoPulse-IFX compared to fresh IFX stock. The average ± SD (n = 10) IFX content of VAL1, VAL2, and VAL3 was 96 ± 2%, 97 ± 3%, and 96 ± 2%, respectively, and complied with the European Pharmacopeia (Ph. Eur.) requirements for Content Uniformity. The average ± SD (n = 3) ColoPulse-IFX potency was 105 ± 4%, 96 ± 4%, and 97 ± 5%, respectively, compared to fresh IFX stock. The IFX release profile from the tablet core was complete (≥85%) after 10 min in simulated ileum medium. The in vitro coating performance of ColoPulse-IFX showed that the formulation was targeted to the simulated ileo-colonic region. Stability data showed that ColoPulse-IFX was stable for up to 6 months stored at 25 °C/60% RH. Based on these results, the production process can be considered validated and its application is discussed in light of the rationale and available evidence for the topical treatment of IBD with IFX.
Plant Development and Organogenesis: From Basic Principles to Applied Research
Authors: Frugis Giovanna
Published: August 2019

The way plants grow and develop organs significantly impacts the overall performance and yield of crop plants. The basic knowledge now available in plant development has the potential to help breeders in generating plants with defined architectural features to improve productivity. Plant translational research effort has steadily increased over the last decade, due to the huge increase in the availability of crop genomic resources and Arabidopsis-based sequence annotation systems. However, a consistent gap between fundamental and applied science has yet to be filled. One critical point is often the unreadiness of developmental biologists on one side, to foresee agricultural applications for their discoveries, and of the breeders on the other, to exploit gene function studies to apply candidate gene approaches when advantageous. In this Special Issue, developmental biologists and breeders make a special effort to reconcile research on basic principles of plant development and organogenesis with its applications to crop production and genetic improvement. Fundamental and applied science contributions interwine and chase each other, giving the reader different but complementary perpectives from only apparently distant corners of the same world.
Circular and Micro RNAs from Arabidopsis thaliana Flowers Are Simultaneously Isolated from AGO-IP Libraries
Authors: Frydrych Capelari Érika , da Fonseca Guilherme Cordenonsi , Guzman Frank , Margis Rogerio
Published: August 2019

Competing endogenous RNAs (ceRNAs) are natural transcripts that can act as endogenous sponges of microRNAs (miRNAs), modulating miRNA action upon target mRNAs. Circular RNAs (circRNAs) are one among the various classes of ceRNAs. They are produced from a process called back-splicing and have been identified in many eukaryotes. In plants, their effective action as a miRNA sponge was not yet demonstrated. To address this question, public mRNAseq data from Argonaute-immunoprecipitation libraries (AGO-IP) of Arabidopsis thaliana flowers were used in association with a bioinformatics comparative multi-method to identify putative circular RNAs. A total of 27,812 circRNAs, with at least two reads at the back-splicing junction, were identified. Further analyses were used to select those circRNAs with potential miRNAs binding sites. As AGO forms a ternary complex with miRNA and target mRNA, targets count in AGO-IP and input libraries were compared, demonstrating that mRNA targets of these miRNAs are enriched in AGO-IP libraries. Through this work, five circRNAs that may function as miRNA sponges were identified and one of them were validated by PCR and sequencing. Our findings indicate that this post-transcriptional regulation can also occur in plants.
Open-Cell Rigid Polyurethane Foams from Peanut Shell-Derived Polyols Prepared under Different Post-Processing Conditions
Authors: Zhang Guangyu , Wu Yumin , Chen Weisheng , Han Dezhi , Lin Xiaoqi , Xu Gongchen , Zhang Qinqin
Published: August 2019

Bio-based polyurethane materials with abundant open-cells have wide applications because of their biodegradability for addressing the issue of environmental conservation. In this work, open-cell rigid polyurethane foams (RPUFs) were prepared with bio-based polyols (BBPs) derived from the liquefaction of peanut shells under different post-processing conditions. The influences of the neutralization procedure and filtering operation for BBPs on the foaming behaviors, density, dimensional stability, water absorption, swelling ratio, compressive strength, and microstructure of RPUFs were investigated intensively. The results revealed that a small amount of sulfuric acid in the polyols exhibited a great impact on physical and chemical properties of RPUFs while the filtering operation for those polyols had a slight effect on the above properties. The RPUFs prepared from neutralized BBPs possessed higher water absorption, preferable dimensional stability and compression strength than that fabricated from the non-neutralized BBPs. Moreover, the prepared RPUFs exhibited preferable water absorption of 636–777%, dimensional stability of <0.5%, compressive strength of >200 KPa, lower swelling rate of ca. 1%, as well as uniform cell structure with superior open-cell rate, implying potential applications in floral foam.
Preparation and Characterization of PVA/PDDA/Nano-Zirconia Composite Anion Exchange Membranes for Fuel Cells
Authors: Samsudin Asep Muhamad , Hacker Viktor
Published: August 2019

Anion exchange membranes (AEMs) contribute significantly to enhance the performance and efficiency of alkaline polymer electrolyte fuel cells (APEFCs). A sequence of composite anion exchange membranes (AEMs) consisting of poly(vinyl alcohol) (PVA), poly(diallyldimethylammonium chloride) (PDDA), and nano-zirconia (NZ) has been prepared by a solution casting technique. The effect of zirconia mass ratio on attribute and performance of composite AEMs was investigated. The chemical structures, morphology, thermal, and mechanical properties of AEMs were characterized by FTIR, SEM, thermogravimetric analysis, and universal testing machine, respectively. The performance of composite AEMs was verified using water uptake, swelling degree, ion-exchange capacity, and OH− conductivity measurement. The nano-zirconia was homogeneously dispersed in the PVA/PDDA AEMs matrix. The mechanical properties of the composite AEMs were considerably enhanced with the addition of NZ. Through the introduction of 1.5 wt.% NZ, PVA/PDDA/NZ composite AEMs acquired the highest hydroxide conductivity of 31.57 mS·cm−1 at ambient condition. This study demonstrates that the PVA/PDDA/NZ AEMs are a potential candidate for APEFCs application.
Stimuli-Responsive Lyotropic Liquid Crystalline Nanosystems with Incorporated Poly(2-Dimethylamino Ethyl Methacrylate)-b-Poly(Lauryl Methacrylate) Amphiphilic Block Copolymer
Authors: Chountoulesi Maria , Pippa Natassa , Chrysostomou Varvara , Pispas Stergios , Chrysina Evangelia D. , Forys Aleksander , Otulakowski Lukasz , Trzebicka Barbara , Demetzos Costas
Published: August 2019

There is an emerging need to evolve the conventional lyotropic liquid crystalline nanoparticles to advanced stimuli-responsive, therapeutic nanosystems with upgraded functionality. Towards this effort, typically used stabilizers, such as Pluronics®, can be combined or replaced by smart, stimuli-responsive block copolymers. The aim of this study is to incorporate the stimuli-responsive amphiphilic block copolymer poly(2-(dimethylamino)ethyl methacrylate)-b-poly(lauryl methacrylate) (PDMAEMA-b-PLMA) as a stabilizer in lipidic liquid crystalline nanoparticles, in order to provide steric stabilization and simultaneous stimuli-responsiveness. The physicochemical and morphological characteristics of the prepared nanosystems were investigated by light scattering techniques, cryogenic-transmission electron microscopy (cryo-TEM), X-ray diffraction (XRD) and fluorescence spectroscopy. The PDMAEMA-b-PLMA, either individually or combined with Poloxamer 407, exhibited different modes of stabilization depending on the lipid used. Due to the protonation ability of PDMAEMA blocks in acidic pH, the nanoparticles exhibited high positive charge, as well as pH-responsive charge conversion, which can be exploited towards pharmaceutical applications. The ionic strength, temperature and serum proteins influenced the physicochemical behavior of the nanoparticles, while the polymer concentration differentiated their morphology; their micropolarity and microfluidity were also evaluated. The proposed liquid crystalline nanosystems can be considered as novel and attractive pH-responsive drug and gene delivery nanocarriers due to their polycationic content.
Understanding Neighbourhoods as Multifaith Spaces
Authors: Prideaux Melanie
Published: August 2019

This paper asked whether it is possible to understand neighbourhoods as ‘multifaith spaces’ and whether doing so helps us think differently about neighbourhoods or about multifaith spaces. By referring to the English context, this paper explored the significance of the local and the demotic in the experience of religious diversity, the construction of space as ‘multifaith’, and the practice and experience of interfaith dialogue. It concluded that thinking of neighbourhoods as multifaith spaces both challenges how multifaith spaces are identified and articulated and deepens the discussion of neighbourhoods as key to the experience of living with religious diversity.
River Goddesses, Personhood and Rights of Nature: Implications for Spiritual Ecology
Authors: Alley Kelly D.
Published: August 2019

Designating rights for nature is a potentially powerful way to open up the dialogue on nature conservation around the world and provide enforcement power for an ecocentric approach. Experiments using a rights-based framework have combined in-country perspectives, worldviews, and practices with legal justifications giving rights to nature. This paper looks at a fusion of legal traditions, religious worldviews, and practices of environmental protection and advocacy in the context of India. It takes two specific legal cases in India and examines the recent high-profile rulings designating the rivers Ganga, Yamuna, and their tributaries and glaciers as juristic persons. Although the rulings were stayed a few months after their issuance, they are an interesting bending of the boundaries of nature, person, and deity that produce Ganga and Yamuna as vulnerable prototypes. This paper uses interview data focusing on these cases and document and archival data to ask whether legal interventions giving rights to nature can become effective avenues for environmental activism and spiritual ecology. The paper also assesses whether these legal cases have promoted Hindu nationalism or ‘Hindutva lite’.
The Impacts of Growth and Environmental Parameters on Solar-Induced Chlorophyll Fluorescence at Seasonal and Diurnal Scales
Authors: Liu Leizhen , Zhao Wenhui , Wu Jianjun , Liu Shasha , Teng Yanguo , Yang Jianhua , Han Xinyi
Published: August 2019

Solar-induced chlorophyll fluorescence (SIF) is considered to be a potential indicator of photosynthesis. However, the impact of growth and environmental parameters on SIF at different time-scales remains unclear, which has greatly restricted the application of SIF in detecting photosynthesis variations. Thus, in this study, the impact of growth and environmental parameters on SIF was thoroughly clarified. Here, continuous time series of canopy SIF (760 nm, F760) over wheat and maize was measured based on an automated spectroscopy system. Meanwhile, field measurements of growth and environmental parameters were also collected using commercial-grade devices. Relationships of these parameters with F760, apparent SIF (F760/solar radiance, AF760), and SIF yield (F760/canopy radiance of 685 nm, Fy760) were analyzed using principal component analysis (PCA) and Pearson correlation to reveal their impacts on SIF. Results showed that F760 at seasonal and diurnal scales were mainly driven by solar radiation (SWR), leaf area index (LAI), chlorophyll content (Chl), mean leaf inclination angle (MTA), and relative water content (RWC). Other environmental parameters, including air temperature (Ta), relative humidity (Rh), vapor pressure deficit (VPD), and soil moisture (SM), contribute less to the variation of seasonal or diurnal F760. AF760 and Fy760 are likely to be less dependent on Ta, Rh, and VPD due to the removal of the impact from SWR, but an enhanced relationship of AF760 (and Fy760) with SM was observed, particularly under water stress. Compared with F760, wheat AF760 was better correlated to LAI and RWC as expected, while maize AF760 did not show an enhanced relationship with all growth parameters, probably due to its complicated canopy structure. The relationship of wheat Fy760 with canopy structure parameters was further reduced, except for maize measurements. Furthermore, SM-induced water stress and phenological stages should be taken into consideration when we interpret the seasonal and diurnal patterns of SIF since they were closely related to photosynthesis and plant growth (e.g., LAI in our study). To our knowledge, this is the first exploration of the impacts of growth and environmental parameters on SIF based on continuous ground measurements, not only at a seasonal scale but also at a diurnal scale. Our results could provide deep insight into the variation of SIF signals and also promote the further application of SIF in the health assessments of terrestrial ecosystems.
Dancing Salsa with Machines—Filling the Gap of Dancing Learning Solutions
Authors: Romano Gianluca , Schneider Jan , Drachsler Hendrik
Published: August 2019

Dancing is an activity that positively enhances the mood of people that consists of feeling the music and expressing it in rhythmic movements with the body. Learning how to dance can be challenging because it requires proper coordination and understanding of rhythm and beat. In this paper, we present the first implementation of the Dancing Coach (DC), a generic system designed to support the practice of dancing steps, which in its current state supports the practice of basic salsa dancing steps. However, the DC has been designed to allow the addition of more dance styles. We also present the first user evaluation of the DC, which consists of user tests with 25 participants. Results from the user test show that participants stated they had learned the basic salsa dancing steps, to move to the beat and body coordination in a fun way. Results also point out some direction on how to improve the future versions of the DC.
Can We Use Satellite-Based FAPAR to Detect Drought?
Authors: Peng Jian , Muller Jan-Peter , Blessing Simon , Giering Ralf , Danne Olaf , Gobron Nadine , Kharbouche Said , Ludwig Ralf , Müller Ben , Leng Guoyong , You Qinglong , Duan Zheng , Dadson Simon
Published: August 2019

Drought in Australia has widespread impacts on agriculture and ecosystems. Satellite-based Fraction of Absorbed Photosynthetically Active Radiation (FAPAR) has great potential to monitor and assess drought impacts on vegetation greenness and health. Various FAPAR products based on satellite observations have been generated and made available to the public. However, differences remain among these datasets due to different retrieval methodologies and assumptions. The Quality Assurance for Essential Climate Variables (QA4ECV) project recently developed a quality assurance framework to provide understandable and traceable quality information for Essential Climate Variables (ECVs). The QA4ECV FAPAR is one of these ECVs. The aim of this study is to investigate the capability of QA4ECV FAPAR for drought monitoring in Australia. Through spatial and temporal comparison and correlation analysis with widely used Moderate Resolution Imaging Spectroradiometer (MODIS), Satellite Pour l’Observation de la Terre (SPOT)/PROBA-V FAPAR generated by Copernicus Global Land Service (CGLS), and the Standardized Precipitation Evapotranspiration Index (SPEI) drought index, as well as the European Space Agency’s Climate Change Initiative (ESA CCI) soil moisture, the study shows that the QA4ECV FAPAR can support agricultural drought monitoring and assessment in Australia. The traceable and reliable uncertainties associated with the QA4ECV FAPAR provide valuable information for applications that use the QA4ECV FAPAR dataset in the future.