bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-messenger astrophysics of black holes and neutron stars as probed by ground-based gravitational wave detectors: from present to future
Authors: Corsi Alessandra , Barsotti Lisa , Berti Emanuele , Evans Matthew , Gupta Ish , Kritos Konstantinos , Kuns Kevin , Nitz Alexander H. , Owen Benjamin J. , Rajbhandari Binod , Read Jocelyn , Sathyaprakash Bangalore S. , Shoemaker David H. , Smith Joshua R. , Vitale Salvatore
Published: May 2024

The ground-based gravitational wave (GW) detectors LIGO and Virgo have enabled the birth of multi-messenger GW astronomy via the detection of GWs from merging stellar-mass black holes (BHs) and neutron stars (NSs). GW170817, the first binary NS merger detected in GWs and all bands of the electromagnetic spectrum, is an outstanding example of the impact that GW discoveries can have on multi-messenger astronomy. Yet, GW170817 is only one of the many and varied multi-messenger sources that can be unveiled using ground-based GW detectors. In this contribution, we summarize key open questions in the astrophysics of stellar-mass BHs and NSs that can be answered using current and future-generation ground-based GW detectors, and highlight the potential for new multi-messenger discoveries ahead.
L-LOP/LOPP for the treatment of canine gastrointestinal/hepatosplenic lymphoma
Authors: Lai Yu Ying , Horta Rodrigo dos Santos , Almendros Angel , Ha Patrick W. Y. , Giuliano Antonio
Published: May 2024

Canine gastrointestinal (GI) and hepatosplenic (HS) high-grade (large cell) lymphomas are uncommon forms of canine lymphomas, with a very poor response to chemotherapy and a very poor prognosis. Currently, there are no established effective chemotherapy protocols for canine GI/HS lymphomas. This case series aimed to retrospectively evaluate the efficacy of lomustine-based protocols L-LOP (L-asparaginase, lomustine, vincristine, and prednisolone) and L-LOPP (with the addition of procarbazine) for treatment of canine GI/HS lymphomas. Medical records of dogs with cytologically or histologically diagnosed lymphoma at CityU Veterinary Medical Centre from 2019 to 2022 were retrospectively reviewed. The L-LOP/LOPP treatment protocol was well tolerated with rare severe adverse events. Median progression-free survival for GI and HS lymphoma was 56 days (range, 10–274 days) and 57 days (range 8–135 days) respectively; while median survival time for GI and HS lymphoma was 93 days (range 10–325 days) and 210 days (range 8–240 days) respectively.
Delineation of chicken immune markers in the era of omics and multicolor flow cytometry
Authors: Härtle Sonja , Sutton Kate , Vervelde Lonneke , Dalgaard Tina S.
Published: May 2024

Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.
Determination of prevalence of subclinical mastitis, characterization of intra-mammary infection-causing bacteria, and antibiotic susceptibility in dairy camels in Jigjiga City, Somali region, Ethiopia
Authors: Jama Mohamoud Mohamed , Hussein Hassan Abdi , Darod Ziad Abdulahi , Ahad Abdullahi Adan
Published: May 2024

Background

Subclinical mastitis in camels, an inflammation of the udder without visible signs, can reduce milk quality and raise bacteria levels. Regular monitoring of camel milk is crucial for consumer safety.

Methods

A cross sectional study was conducted in Jigjiga city, Ethiopia to investigate the prevalence and characteristics of subclinical mastitis in she-camels. The study included 244 lactating she-camels from three privately-owned camel dairy farms, and a questionnaire survey was conducted with 60 camel owners.

Results

The overall prevalence of subclinical mastitis in she-camels was 10.6% (26/244), with no significant difference among the studied dairy farms. Risk factors that influenced the result of California Mastitis Test (CMT) included age and udder and leg hygiene. The study revealed that S. aureus was the most prevalent bacterium among the isolated bacteria, with a prevalence rate of 34.5%. This was followed by S. agalactiae, S. dysgalactiae, and Pasteurella multocida, with prevalence rates of 29.8, 19.4, and 16.2%, respectively. Among the isolated bacteria, 84.5% were sensitive to Erythromycin, 60% to Streptomycin, 44.7% to Oxytetracycline, and 36.7% to Tetracycline. Interviews with camel owners revealed that 66.7% used mixed herd grazing methods and reported feed shortage. Treatment practices for sick camels included modern veterinary drugs, traditional medicines, or a combination of both. The owners of camel dairy farms did not maintain proper hygiene practices during milking, such as not using soap when washing hands.

Conclusion

Addressing camel mastitis necessitates access to alternative drugs, comprehensive herder training, and enhanced management practices.
Peripartal changes of metabolic and hormonal parameters in Romanian spotted cows and their relation with retained fetal membranes
Authors: Rafa Horatiu , Oroian Ioan , Cozma Oana Maria , Morohoschi Andreea Georgiana , Dumitraș Daria Antonia , Ștefănuț Cristina Laura , Neagu Daniela , Borzan Alex , Andrei Sanda
Published: May 2024

This clinical study investigates various metabolic and physiological parameters in dairy cows during puerperium. Retained fetal membranes (RFM) is a significant postpartum complication that can affect the overall health, fertility and productivity of dairy cattle. The research focuses on changes in total proteins, albumin, glucose, triglycerides, total cholesterol, aspartate aminotransferase (AST) and alanine aminotransferase (ALT), cortisol, insulin, and insulin-like growth factor 1 (IGF-1) levels among cows experiencing normal post-partum period (NP) and those with RFM. A significant increase in protein levels was noted during the post-partum period in the RFM group, indicating physiological impacts of RFM at this stage. Albumin levels showed significant differences, highlighting a significant biological effect of RFM in the post-partum period. Glucose levels varied significantly in the weeks leading to parturition, suggesting altered metabolic states in cows that suffered RFM. Triglyceride and cholesterol levels were significantly higher during the antepartum period in the group that experienced reproductive failure, indicating substantial alterations in lipid metabolism which could herald the apparition of RFM. AST and ALT levels provided insights into cellular stress and liver function, with significant increases noted around parturition which could be attributed to the substantial physiological strain of parturition itself. Cortisol levels were higher in RFM cows 2 weeks before parturition, which could indicate an increasing stress response or a physiological preparation for the upcoming labor, and may be more pronounced in cows predisposed to RFM. Insulin levels decreased significantly before and at parturition in RFM cows, indicating a strong energy deficit. IGF-1 levels decreased significantly in RFM cows after parturition. Significant changes in metabolic parameters, such as glucose, triglycerides, and cholesterol levels, delineate the pronounced metabolic challenges faced by cows with RFM. The study elucidates that while some variations are noted as parturition approaches, the most substantial impacts attributable to RFM on metabolic and physiological parameters occur after parturition. These changes may have implications for the health, recovery, and productivity of cows postpartum, suggesting the need for targeted management strategies to mitigate the effects of RFM.
Assessment of Mitochondrial Respiration During Hypothermic Storage of Liver Biopsies Following Normothermic Machine Perfusion
Authors: Hofmann Julia , Kofler Alexander , Schartner Melanie , Buch Madita L. , Hermann Martin , Zelger Bettina , Öfner Dietmar , Oberhuber Rupert , Hautz Theresa , Schneeberger Stefan , Meszaros Andras T.
Published: May 2024

Organ quality can be assessed prior to transplantation, during normothermic machine perfusion (NMP) of the liver. Evaluation of mitochondrial function by high-resolution respirometry (HRR) may serve as a viability assessment concept in this setting. Freshly collected tissue is considered as optimal sample for HRR, but due to technical and personnel requirements, more flexible and schedulable measurements are needed. However, the impact of cold storage following NMP before processing biopsy samples for mitochondrial analysis remains unknown. We aimed at establishing an appropriate storage protocol of liver biopsies for HRR. Wedge biopsies of 5 human livers during NMP were obtained and assessed by HRR. Analysis was performed after 0, 4, 8, and 12 h of hypothermic storage (HTS) in HTK organ preservation solution at 4°C. With HTS up to 4 h, mitochondrial performance did not decrease in HTS samples compared with 0 h (OXPHOS, 44.62 [34.75–60.15] pmol·s−1·mg wet mass−1 vs. 43.73 [40.69–57.71], median [IQR], p > 0.999). However, at HTS beyond 4 h, mitochondrial respiration decreased. We conclude that HTS can be safely applied for extending the biopsy measurement window for up to 4 h to determine organ quality, but also that human liver respiration degrades beyond 4 h HTS following NMP.
Investigation of Spray Characteristics for Detonability: A Study on Liquid Fuel Injector and Nozzle Design
Authors: Choi Myeung Hwan , Oh Yoojin , Park Sungwoo
Published: May 2024

Detonation engines are gaining prominence as next-generation propulsion systems that can significantly enhance the efficiency of existing engines. This study focuses on developing an injector utilizing liquid fuel and a gas oxidizer for application in detonation engines. In order to better understand the spray characteristics suitable for the pulse detonation engine (PDE) system, an injector was fabricated by varying the Venturi nozzle exit diameter ratio and the geometric features of the fuel injection hole. Analysis of high-speed camera images revealed that the Venturi nozzle exit diameter ratio plays a crucial role in determining the characteristics of air-assist or air-blast atomization. Under the conditions of an exit diameter ratio of Re/Ri = 1.0, the formation of a liquid film at the exit was observed, and it was identified that the film’s length is influenced by the geometric characteristics of the fuel injection hole. The effect of the fuel injection hole and Venturi nozzle exit diameter ratio on SMD was analyzed by using droplet diameter measurement. The derived empirical correlation indicates that the atomization mechanism varies depending on the Venturi nozzle exit diameter ratio, and it also affects the distribution of SMD. The characteristics of the proposed injector, its influence on SMD, and its velocity, provide essential groundwork and data for the design of detonation engines employing liquid fuel.
Evaluation of Heavy Metal Contamination and Associated Human Health Risk in Soils around a Battery Industrial Zone in Henan Province, Central China
Authors: Zhang Jingjing , Jiao Qiujuan , Wu Yong , Liu Haitao , Yu Peiyi , Liu Deyuan , Hua Dangling , Song Jia
Published: May 2024

This research investigated the contamination characteristics, sources, and health risks of five metals in soils from two villages named DK and SXC, downstream from a battery industry hub in Xinxiang city, Henan Province, China. The average concentrations of Cd, Pb, Ni, Cu, and Zn in DK were 5.93, 41.31, 71.40, 62.20, and 115.83 mg/kg, respectively, and in SXC were 2.04, 30.41, 41.22, 36.18, and 96.04 mg/kg, respectively. The single factor pollution index (Pi) revealed a consistent descending order of Cd > Cu > Zn > Ni > Pb in DK and SXC. The geo-accumulation index (Igeo) indicated that the Cd pollution in DK was extreme, and in SXC was at a heavy to extreme level. The potential ecological risk index (PERI) indicated that Cd presented a significantly high ecological risk while it was low for other metals. Principal component analysis classified them into the anthropogenic origin of Cd and common mixed origin of others. The elevated levels and pollution load of heavy metals with closer proximity to the battery factory imply that the factory is a probable source of contamination. Overall, the health risks posed by heavy metals were more pronounced for local children compared to adults, with Cd being the primary contributor to both pollution and health risks. This investigation provides a crucial basis for the heavy metal pollution management and related risk prevention in areas affected by electronic waste irrigation.
Enhancing Broiler Weight Estimation through Gaussian Kernel Density Estimation Modeling
Authors: Oh Yumi , Lyu Peng , Ko Sunwoo , Min Jeongik , Song Juwhan
Published: May 2024

The management of individual weights in broiler farming is not only crucial for increasing farm income but also directly linked to the revenue growth of integrated broiler companies, necessitating prompt resolution. This paper proposes a model to estimate daily average broiler weights using time and weight data collected through scales. In the proposed model, a method of self-adjusting weights in the bandwidth calculation formula is employed, and the daily average weight representative value is estimated using KDE. The focus of this study is to contribute to the individual weight management of broilers by intensively researching daily fluctuations in average broiler weight. To address this, weight and time data are collected and preprocessed through scales. The Gaussian kernel density estimation model proposed in this paper aims to estimate the representative value of the daily average weight of a single broiler using statistical estimation methods, allowing for self-adjustment of bandwidth values. When applied to the dataset collected through scales, the proposed Gaussian kernel density estimation model with self-adjustable bandwidth values confirmed that the estimated daily weight did not deviate beyond the error range of ±50 g compared with the actual measured values. The next step of this study is to systematically understand the impact of the broiler environment on weight for sustainable management strategies for broiler demand, derive optimal rearing conditions for each farm by combining location and weight data, and develop a model for predicting daily average weight values. The ultimate goal is to develop an artificial intelligence model suitable for weight management systems by utilizing the estimated daily average weight of a single broiler even in the presence of error data collected from multiple weight measurements, enabling more efficient automatic measurement of broiler weight and supporting both farms and broiler demand.
Perceived Benefit, Policy Incentive and Farmers’ Organic Fertilizer Application in Protected Areas
Authors: Yang Jie , Su Kaiwen , Zhang Ziyi , Guo Sihan , Hou Yilei , Wen Yali
Published: May 2024

The green production behaviors of farmers in protected areas (PAs) can reduce environmental disturbances and contribute to the effectiveness of PAs. Based on a survey of 708 farmers in Wuyishan National Park (WNP) and Crested Ibis Nature Reserve (CINR), we studied the influence of perceived benefit and policy incentive on farmers’ organic fertilizer application. Results: (1) Perceived benefit, subsidies policy, and propaganda policy significantly promoted farmers’ organic fertilizer application, with perceived benefit having the strongest effect, followed by subsidies policy. The influence of restriction policy was not significant. (2) Perceived benefit, subsidies policy, and propaganda policy indirectly influenced organic fertilizer application through ecological awareness. (3) In WNP, perceived benefit had the strongest effect on organic fertilizer application, and subsidies policy significantly enhanced farmers’ ecological awareness and influenced their behaviors. In contrast, subsidies policy had the strongest effect on organic fertilizer application, but did not significantly enhance farmers’ ecological awareness in CINR. These results offer valuable insights for promoting green production behaviors of farmers in PAs. This study implies that there must be a pathway for the realization of ecological value in ecological protection. Ecological value realization is the endogenous motivation for farmers’ sustainable green production behaviors. However, government subsidies and guidance are also essential in the early stage.
Research on a Variable Universe Control Method and the Performance of Large Sprayer Active Suspension Based on an Artificial Fish Swarm Algorithm–Back Propagation Fuzzy Neural Network
Authors: Yang Fan , Liu Lei , Zhang Yanan , Du Yuefeng , Mao Enrong , Zhu Zhongxiang , Li Zhen
Published: May 2024

In view of the typical requirements of large high-clearance sprayers, such as those operating in poor road conditions for farmland plant protection and at high operation speeds, reducing the vibration of sprayer suspension systems has become a research hotspot. In this study, the hydro-pneumatic suspension (HPS) of large high-clearance sprayers was taken as the object, and a variable universe T-S fuzzy controller with real vehicle vibration data as input was proposed to control suspension motion in real time. Different from traditional semi-active suspension, based on the characteristics of variable universe extension factors, a training method combining the artificial fish swarm algorithm and the back propagation algorithm was used to establish a fuzzy neural network controller with precise input to optimize the variable universe. Then, the time-domain and frequency-domain response characteristics of HPS were analyzed by simulating the special road conditions typical of farmland. Finally, the field performance of the sprayer equipped with the new controller was tested. The results show that the error rate of the AFSA-BP algorithm in training the FNN could be reduced to 3.9%, and compared with a passive suspension system, the T-S fuzzy controller improved the effects of spring mass acceleration, pitch angle acceleration, and roll angle acceleration by 18.3%, 23.3%, and 27.7%, respectively, verifying the effectiveness and engineering practicality of the active controller in this study.
Bulked Segregant Analysis by Sequencing-Based Genetic Mapping of the Green Spotted Fruit Rind Regulating Locus in Wild Melon XNM020 Reveals Four Possible Candidate Genes
Authors: Zhou Yuqing , Yang Yuqing , Xiang Yachen , Cui Haibing , Zhou Yuan , Liu Hanqiang , Zhang Huijun , Pan Yupeng
Published: May 2024

Fruit rind patterns are vital commercial quality traits in melon, in which the spotted or striped fruit rinds contribute to the commercial value of melon and can directly affect the choice of consumers. Although the spotted or non-spotted fruit rind pattern was studied in several cultivated melon accessions, the inheritance and regulating locus of this trait in wild melons are still unknown. Therefore, in this study, the inheritance and regulating loci of the green spotted fruit rind in a wild melon accession XNM020 were explored with F2 segregating populations derived from crossing between XNM020 and a cultivated melon XNM125. Segregating ratios of phenotypic data indicated that the green spotted fruit rind in XNM020 has a monogenic dominant inheritance. BSA-Seq showed that two potential genomic regions on chromosomes 4 (from 0.00 to 2.97 Mb) and 5 (from 0.00 to 2.34 Mb) regulate the formation of the green spotted fruit rind in wild melon XNM020. According to the annotations of polymorphic SNPs (single-nucleotide polymorphisms) and small InDels (insertions and deletions) in target genomic regions and the predicted gene functions, four genes MELO3C003316, MELO3C003375, MELO3C003388, and MELO3C014660 regulating chloroplast development or chlorophyll biosynthesis may be the best candidate genes. The results of this study enriched the inheritances of spotted fruit rinds in melon and also provided target genomic regions for marker-assisted selection breeding of melon focusing on fruit rinds.
Optimizing Pathogen Control through Mixed Cocoa–Plantain Agroecosystems in the Ecuadorian Coastal Region
Authors: Vera-Velez Roy , Ramos-Veintimilla Raul , Grijalva-Olmedo Jorge
Published: May 2024

Mixed production systems play a vital role in the economic sustainability and ecological balance of agroecosystems. Cocoa and plantain are key crops in Ecuador but face phytosanitary challenges, like witches’ broom and black sigatoka diseases, especially when cultivated under monocropping systems. Combining habitat manipulation with adaptive pathogen management (APM) strategies can mitigate these challenges, but their efficacy in mixed cropping systems remains unclear. This study investigates disease and pest incidence in mixed cocoa–plantain systems during the establishment phase, focusing on the impact of spatial arrangements. Mixed agroecosystems showed a lower witches’ broom incidence in cocoa than monocultures. Whereas, in plantain, there was a consistent black sigatoka incidence across spatial arrangements but a lower infection rate per leaf within mixed systems. We found varied nematode populations with monocultures hosting the highest root damage due to phytoparasitic nematodes. Weevil populations were also influenced by spatial arrangements with monocultures among the highest. Overall, mixed agroecosystems influence disease and pest incidence, potentially hindering pathogen spread. Plantain–cocoa associations reduce disease incidence in cocoa but may not affect the overall incidence of black sigatoka in plantain, at least during the establishment phase. Continued monitoring is crucial for understanding the long-term impacts and microclimatic effects on pest populations that could offer sustainable pest management strategies, reducing the reliance on chemical pesticides.
Analysis of the Factors Affecting the Deposition Coverage of Air-Assisted Electrostatic Spray on Tomato Leaves
Authors: Guo Jili , Dong Xiaoya , Qiu Baijing
Published: May 2024

In order to investigate the effects of various factors (charging voltage, spray distance and spray pattern) on the deposition coverage of tomato leaves, the Box–Behnken surface response methodology was used to design an outdoor air-assisted electrostatic spraying experiment with three factors and three levels. The deposition coverage of tomato leaves in the upper, middle and lower layers was collected under different polarity charging voltages (0, +10 kV, −10 kV), spray distances (1, 3, 5 m) and spray patterns (ascending spray, descending spray, fixed height spray). Regression analysis and variance analysis were performed on the experimental data to determine the optimal working parameters. The results showed that (1) spray distance is the most important factor affecting the droplet coverage rate in the process of air-assisted electrostatic spraying; (2) the droplet coverage rate of air-assisted electrostatic spraying is optimal when the charging voltage polarity is negative voltage, the spray distance is 2.75 m, and the spray pattern is descending spray. The following conclusions were obtained. (1) In air-assisted electrostatic spraying, the distribution of air flow had the greatest effect on droplet deposition on tomato leaf surface. (2) Compared with air-assisted non-electrostatic spray, air-assisted electrostatic spray had a better deposition effect.
Phosphorus Supply to Plants of Vaccinium L. Genus: Proven Patterns and Unexplored Issues
Authors: Struchkova Irina V. , Mikheev Vyacheslav S. , Berezina Ekaterina V. , Brilkina Anna A.
Published: May 2024

Phosphorus availability is a serious problem for plants growing and grown in acidic soils of bogs, poor in macronutrients. The application of phosphorus fertilizers to such soils is unprofitable because of the physical and chemical properties of these soils, where phosphate is firmly bound to organic and inorganic compounds and becomes inaccessible to plants. Plants of the Vaccinium genus both from natural stands and commercial plantations may suffer from phosphorus deficiency, so they need to have a number of adaptations that allow them to efficiently extract phosphorus. This review addresses the following issues in relation to plants of the Vaccinium genus: sources of phosphorus for plants; the release of phosphate ions from soil components; the transport of phosphate ions into plants; and the importance of mycorrhiza in supplying phosphorus to plants. Thus, we sought to draw researchers’ attention to sources and routes of phosphorus supply of plants of the Vaccinium genus and its unexplored aspects.
Extraction of Corn Plant Phenotypic Parameters with Keypoint Detection and Stereo Images
Authors: Gao Yuliang , Li Zhen , Li Bin , Zhang Lifeng
Published: May 2024

Corn is a global crop that requires the breeding of superior varieties. A crucial aspect of the breeding process is the accurate extraction of phenotypic parameters from corn plants. The existing challenges in phenotypic parameter extraction include low precision, excessive manual involvement, prolonged processing time, and equipment complexity. This study addresses these challenges by opting for binocular cameras as the data acquisition equipment. The proposed stereo corn phenotype extraction algorithm (SCPE) leverages binocular images for phenotypic parameter extraction. The SCPE consists of two modules: the YOLOv7-SlimPose model and the phenotypic parameter extraction module. The YOLOv7-SlimPose model was developed by optimizing the neck component, refining the loss function, and pruning the model based on YOLOv7-Pose. This model can better detect bounding boxes and keypoints with fewer parameters. The phenotypic parameter extraction module can construct the skeleton of the corn plant and extract phenotypic parameters based on the coordinates of the keypoints detected. The results showed the effectiveness of the approach, with the YOLOv7-SlimPose model achieving a keypoint mean average precision (mAP) of 96.8% with 65.1 million parameters and a speed of 0.09 s/item. The phenotypic parameter extraction module processed one corn plant in approximately 0.2 s, resulting in a total time cost of 0.38 s for the entire SCPE algorithm to construct the skeleton and extract the phenotypic parameters. The SCPE algorithm is economical and effective for extracting phenotypic parameters from corn plants, and the skeleton of corn plants can be constructed to evaluate the growth of corn as a reference. This proposal can also serve as a valuable reference for similar functions in other crops such as sorghum, rice, and wheat.
Transcriptome Sequencing and Metabolome Analysis Reveals the Regulatory and Molecular Mechanisms of the Grain Filling Rate in Foxtail Millet (Setaria italica L.)
Authors: Han Yuetao , Zhao Peiyue , Zhao Yuan , Liu Min , Guo Erhu , Wang Guoliang , Zhang Aiying
Published: May 2024

The grain filling rate (GFR) plays a crucial role in determining grain yield. However, the regulatory and molecular mechanisms of the grain filling rate (GFR) in foxtail millet remains unclear. In this study, we found that the GFR of ′Changnong No.47′ (CN47) was significantly higher at 14 DAF (days after flowering) and 21 DAF in comparison to ‘Changsheng 13’ (CS13). Furthermore, CN47 also exhibited higher a thousand-grain weight and yield than CS13. Therefore, RNA-seq and UHPLC-MS/MS were used to conduct transcriptome and metabolome analyses during two stages of grain filling in both cultivars. Conjoint analysis of transcriptomics and metabolomics was adopted in order to analyze the biological processes and functional genes associated with GFR. The results identified a total of 765 differentially expressed genes (DEGs) and 246 differentially accumulated metabolites (DAMs) at the 14 DAF stage, while at the 21 DAF stage, a total of 908 DEGs and 268 DAMs were identified. The integrated analysis of co-mapped DAMs and DEGs revealed enriched pathways, including flavonoid biosynthesis, plant hormone signal transduction, tyrosine metabolism, ATP-binding cassette (ABC) transporters, and beta-Alanine metabolism, as well as stilbenoid, diarylheptanoid, and gingerol biosynthesis. In order to elucidate their potential functions in the context of GFR, we developed a gene–metabolite regulatory network for these metabolic pathways. Notably, we found that some genes associated with ABC transporters and the plant hormone signal transduction pathway were implicated in auxin transport and signal transduction, highlighting the crucial role of auxin during grain filling. These findings provide initial insights into the regulatory and molecular mechanisms underlying GFR in foxtail millet, as well as offering valuable genetic resources for further elucidation of GFR in future studies. The findings have also established a theoretical basis for improving the efficiency of yield breeding in foxtail millet.
Replacement of Corn with Different Levels of Wheat Impacted the Growth Performance, Intestinal Development, and Cecal Microbiota of Broilers
Authors: Liu Luxin , Wang Zilin , Wei Bin , Wang Leilei , Zhang Qianqian , Si Xuemeng , Huang Yanqun , Zhang Huaiyong , Chen Wen
Published: May 2024

Replacing corn with different levels of wheat in the iso-energy and -protein diet of broilers and the impacts on growth performance and intestinal homeostasis of broilers under the condition of supplying the multienzyme complex were evaluated in this study. A total of 480 10-day-old male broilers were assigned randomly to the low-level wheat group (15% wheat and 35.18% corn), the medium-level wheat group (30% and 22.27%), and the high-level wheat group (55.77% wheat without corn) until 21 d. The different levels of wheat supplementation did not affect hepatic function, serum glycolipid profile, or bone turnover. The replacement of corn with 55% wheat in the diet of broilers increased the body weight at 21 d and feed intake during 10 to 21 d (both p < 0.05), with a comparable feed conversion ratio. Compared with the low-wheat group, the dietary addition of medium or high wheat levels notably increased the ratio of villus height to crypt depth in the duodenum (p < 0.05) and the ileal villus height (p < 0.05). Meanwhile, the supplementation of medium and high wheat in the diet increased the proportion of Bacteroidetes, and a diet with high wheat proportion elevated the content of Firmicutes when compared to the low-level wheat group (both p < 0.05). In addition, the diet containing 30–55% wheat enhanced the anti-inflammatory capability in both the ileum and the serum. These findings suggest that the replacement of corn with 55% wheat in the diet improved the growth performance of 21-day-old broilers, which might be linked to the alteration in intestinal morphology and cecal microbiota.
Investigation of PRRS Virus Infection in Hungarian Wild Boar Populations during Its Eradication from Domestic Pig Herds
Authors: Bálint Ádám , Csányi Sándor , Nemes Imre , Bijl Hanna , Szabó István
Published: May 2024

Porcine Reproductive and Respiratory Syndrome (PRRS) significantly impacts the pig farming industry globally, leading to economic losses due to reduced productivity. This study focuses on assessing the presence and impact of PRRS within Hungarian wild boar populations amidst efforts to eradicate the virus from domestic pig herds. We used a combination of serological and virological tests on samples collected from wild boars across Hungary to evaluate the prevalence of PRRS virus and its potential transmission risks to domestic pigs. Our findings reveal a low seropositivity rate in wild boars, suggesting a minimal role of wild boars in the transmission of PRRS to domestic pig populations. Moreover, no relationship was found between domestic pig and wild boar densities, emphasizing the limited interaction and consequent risk of disease spread between these populations. We confirm the effectiveness of Hungary’s PRRS eradication measures among domestic herds and highlight the negligible risk posed by wild boars in re-introducing the PRRS virus.
The Application of Infrared Thermography in the Assessment of BEMER Physical Vascular Therapy on Body Surface Temperature in Racing Thoroughbreds: A Preliminary Study
Authors: Nawrot Karolina , Soroko-Dubrovina Maria , Zielińska Paulina , Dudek Krzysztof , Howell Kevin
Published: May 2024

The study aimed to evaluate the impact of BEMER (Physical Vascular Therapy) on body surface temperature using infrared thermography (IRT) in the distal parts of the forelimbs in Thoroughbreds. The study tested the hypothesis that BEMER therapy leads to an increase in body surface temperature and blood vessel diameter in the distal parts of the forelimbs. The study involved 16 horses, split into 2 groups: active BEMER (n = 8) and sham (n = 8). The active BEMER group had BEMER boots applied to the distal parts of the forelimbs, whereas the sham group had BEMER boots applied without activation of the device. Both groups underwent IRT examination to detect changes in body surface temperature, followed by ultrasonographic examination to assess changes in vein and artery diameter before (BT) and just after (JAT) therapy. The IRT examination was repeated 15 min after BEMER therapy (15AT). There were no significant body surface temperature differences between BT and JAT in any regions of interest (ROIs) in either group. In the active BEMER group, the ROIs did not change significantly at 15AT, compared to the temperatures measured at BT (except for the hooves). At 15AT the temperature of all the ROIs (except the fetlock bone) dropped significantly in the sham group. In the ultrasonographic examination, there was a significant increase in vein and artery diameter in the study group JAT, whereas the sham group had a significant increase only in artery diameter JAT. These results suggest an effect of BEMER on stimulating blood circulation in the distal parts of the forelimbs in clinically healthy horses. IRT did not identify changes in skin surface temperature after BEMER therapy at the distal parts of the forelimbs.
Calf Management: Individual or Paired Housing Affects Dairy Calf Health and Welfare
Authors: McFarland David S. , McFarland Lorna M. , Shaw Darren J. , Macrae Alastair I.
Published: May 2024

Previous research has indicated that preweaned dairy calves reared in pairs compared with individually have improved performance and indicators of animal welfare. One hundred and thirty Holstein female calves completed the trial, with eighty-five being allocated to paired housing and forty-five calves being allocated to individual housing. Daily live weight gain (DLWG), treatments and mortality were recorded throughout the preweaning period. Salivary cortisol, latency to feed and latency to approach a novel object were assessed at batching. There were no significant differences in DLWG, mortality and disease treatments between the average of the pair and the individually housed calves, although the pair-reared calves were quicker to approach the milk feed after batching and interacted more quickly with a novel object. The heaviest born calves within the pair had the highest DLWG from birth to weaning, with a higher percentage of calves approaching the novel object, compared with the lightest born calf within the pair. This study shows that calves within a pair may have significantly different performance and welfare during the preweaning period, with the heavier calf outperforming and displaying less fear and more exploratory behaviour than the lighter calf within a pair.
Viral Diagnosis in Psittacine Birds: A Scientometric and Systematic Review of 47 Years
Authors: Antonio Edma Santos , Fraga Ricardo Evangelista , Silva Janisete Gomes
Published: May 2024

The first reports of viruses in psittacine birds date back to the early 1970s. Here, we elucidate the differences among these previous studies and the advances achieved. The objective of this study was to carry out a comprehensive review using both scientometric and systematic methods to analyze the evolution of published studies on viruses in psittacine birds up to 2022. The search descriptors “virus”, “diagnosis”, and “Psittaciformes” were used to find the articles of interest for this study. A total of 118 articles were manually selected, and the scientometric data were organized using the software VOSviewer® version 1.6.18. The present review revealed that: (i) on average, 2.5 articles/year on the diagnosis of viral infection in psittacine birds were published since 1975; (ii) the most productive research groups are concentrated in three countries: Australia, the United States, and Germany; (iii) the most important virus in psittacine birds is the Circovirus, which causes psittacine beak and feather disease; (iv) the diagnostic method of choice is polymerase chain reaction (PCR); and (v) the most studied psittacine birds were those in the Psittacidae family that were kept in captivity.
Photobiomodulation Inhibits Ischemia-Induced Brain Endothelial Senescence via Endothelial Nitric Oxide Synthase
Authors: Feng Yu , Huang Zhihai , Ma Xiaohui , Zong Xuemei , Tesic Vesna , Ding Baojin , Wu Celeste Yin-Chieh , Lee Reggie Hui-Chao , Zhang Quanguang
Published: May 2024

Recent research suggests that photobiomodulation therapy (PBMT) positively impacts the vascular function associated with various cerebrovascular diseases. Nevertheless, the specific mechanisms by which PBMT improves vascular function remain ambiguous. Since endothelial nitric oxide synthase (eNOS) is crucial in regulating vascular function following cerebral ischemia, we investigated whether eNOS is a key element controlling cerebrovascular function and the senescence of vascular endothelial cells following PBMT treatment. Both rat photothrombotic (PT) stroke and in vitro oxygen–glucose deprivation (OGD)-induced vascular endothelial injury models were utilized. We demonstrated that treatment with PBMT (808 nm, 350 mW/cm2, 2 min/day) for 7 days significantly reduced PT-stroke-induced vascular permeability. Additionally, PBMT inhibited the levels of endothelial senescence markers (senescence green and p21) and antiangiogenic factor (endostatin), while increasing the phospho-eNOS (Ser1177) in the peri-infarct region following PT stroke. In vitro study further indicated that OGD increased p21, endostatin, and DNA damage (γH2AX) levels in the brain endothelial cell line, but they were reversed by PBMT. Intriguingly, the beneficial effects of PBMT were attenuated by a NOS inhibitor. In summary, these findings provide novel insights into the role of eNOS in PBMT-mediated protection against cerebrovascular senescence and endothelial dysfunction following ischemia. The use of PBMT as a therapeutic is a promising strategy to improve endothelial function in cerebrovascular disease.
Micronutrient Antioxidants for Men (Menevit®) Improve Sperm Function by Reducing Oxidative Stress, Resulting in Improved Assisted Reproductive Technology Outcomes
Authors: Ogawa Seiji , Nishizawa Kaori , Shinagawa Masumi , Katagiri Mikiko , Kikuchi Hiroyuki , Kobayashi Hideyuki , Yoshida Hiroaki
Published: May 2024

Oxidative stress (OS) affects men’s health and impairs spermatogenesis. Micronutrient antioxidants are available for male infertility as complemental support; however, their efficacy remains debatable. This study aimed to investigate whether antioxidants can help to reduce sperm OS and improve semen analysis and quality. We included 171 male partners of couples planning to undergo assisted reproductive technology (ART). Male partners, aged 29–41 years, of couples intending to conceive were self-selected to take daily antioxidants (n = 84) containing folic acid and zinc, or not to take antioxidants (n = 52) for 6 months. We analyzed the alterations in serum oxidant levels, sperm parameters, OS, and deoxyribonucleic acid fragmentation after 3 and 6 months. Additionally, implantation, clinical pregnancy, and miscarriage rates after vitrified–warmed embryo transfer were compared between those taking antioxidants and those not taking them after 6 months. In men with high static oxidation–reduction potential (sORP), we observed a significant improvement in sperm concentration and sORP. The high-quality blastocyst rate tended to increase, and implantation and clinical pregnancy rates also significantly increased after 6 months of intervention. The micronutrient antioxidants could improve sperm function by reducing OS and improving ART outcomes. Therefore, micronutrient antioxidants may be a viable treatment option for male infertility.
Investigation of the Degree of Monomer Conversion in Dental Composites through Various Methods: An In Vitro Study
Authors: Ucuncu Musa Kazim , Celiksoz Ozge , Sen Emine , Yucel Yasemin Yucel , Dinc Bircan
Published: May 2024

The degree of monomer conversion (DC) values of three different dental composites were examined using three different methods: surface microhardness (ratio of bottom/top), Fourier-transform infrared (FT-IR), and differential scanning calorimetry (DSC). Two of the dental composites included in the study were nanohybrid (Dentsply Neo Spectra ST HV and Omnichroma), and one was a microhybrid-labeled newly marketed composite containing nanoparticles (Dentac Myra). Composite discs were prepared according to the methodology for all methods and analyzed (2 mm thickness × 5 mm diameter). Surface microhardness values were measured in Vickers Hardness Number (VHN), while FT-IR and DSC values were obtained in percentage (%). Significant differences were observed in both bottom/top surface microhardness values and DC values obtained from FT-IR. However, there was no statistical difference in the ratio of bottom/top microhardness values. Neo Spectra ST HV exhibited superior performance in both microhardness and monomer conversion compared to the other two composites. Newly marketed Myra showed values close to Omnichroma. It was found that the values obtained by the DSC method were parallel to those obtained by FT-IR. In conclusion, the structure of dental composites leads to different mechanical properties. Additionally, DSC measurements and FTIR spectra were found to be complementary techniques for characterizing monomer conversion values.
Aging Impairs Unimanual and Bimanual Hand-Grip Force Control Capabilities
Authors: Ko Do-Kyung , Kang Nyeonju
Published: May 2024

This study examined age-related changes in unimanual and bimanual hand-grip force control capabilities by focusing on absolute and relative outcome measures. Thirty-two older adults and thirty-two younger adults performed isometric hand-grip force control tasks across three hand conditions (unimanual dominant, unimanual non-dominant, and bimanual) and two submaximal targeted levels (10% and 40% of maximal voluntary contraction). Force control performances were evaluated by calculating absolute and relative variables on force accuracy and variability. Furthermore, to determine which force control variables and experimental conditions effectively indicate age-related sensorimotor control deficits, we conducted receiver operating characteristic curve analyses. Older adults demonstrated impaired force control capabilities at 10% of maximal voluntary contraction collapse across the three hand conditions compared with younger adults, and these deficits were identified by both relative force accuracy and relative force variability. Moreover, relative force accuracy showed a good diagnostic quality at 10% of maximal voluntary contraction. These findings suggested that aging may induce unimanual and bimanual hand-grip force control deficits at a lower targeted level, and these motor impairments were sensitively estimated by quantifying relative force control outcome measures that may reflect age-related muscle weakness as compared with absolute measurements.
An Evaluation of the Biometric Parameters and Chemical Composition of the Florets, Leaves, and Stalks of Broccoli Plants Grown in Different Soil Types
Authors: Majkowska-Gadomska Joanna , Kaliniewicz Zdzisław , Francke Anna , Sałata Andrzej , Jadwisieńczak Krzysztof Konrad
Published: May 2024

Soil affects plant growth and development, and it is one of the factors that determine crop yields and quality. Broccoli (Brassica oleracea var. italica Plenck) plants cv. Cezar were grown in an experiment conducted in 2021–2022 on a horticultural farm. The biometric parameters of broccoli plants were determined in the first stage. The chemical composition of the edible parts of broccoli was determined in the second stage, which involved two experimental factors. The first factor was the edible parts of broccoli: florets, leaves, and stalks. The second factor was the effect of soil type on the chemical composition of the edible parts of broccoli. Albic Luvisol (II) had a significant positive effect on floret height and the number of florets. Leaf yield was significantly lower when broccoli plants were grown in Eutric Cambisol (I) compared with Albic Luvisol (II) and degraded chernozem (III). Soil type had no significant influence on the macronutrient content of broccoli florets, leaves, and stalks, but the accumulation of macronutrients varied across the edible plant parts. The content of iron, zinc and manganese in broccoli plants was not significantly affected by soil type, but soil type exerted a significant effect on copper content.
Assessment of Lightweight Concrete Properties with Zinc Oxide Nanoparticles: Structural and Morphological Analyses
Authors: Gonzalez Garcia Ana Silvia , Silva Klein Luis Tomas , Vega Martinez Victor , Alonso Martinez Mar , del Coz-Díaz Juan José
Published: May 2024

In recent decades, the use of nanotechnology has increased in many disciplines. Specifically, in the concrete industry, nanotechnology has been used to develop more eco-efficient solutions. There is a rapidly growing interest in using nanoparticles in concrete to tackle environmental impacts. Among the nanoparticles investigated, zinc oxide (ZnO) shows great potential because of its material properties, such as reactivity, non-toxicity, a hard and rigid structure, photocatalytic and photoluminescence properties, and chemical, electrical, and thermal stabilities. This paper focuses on the analysis of the effect of ZnO nanoparticles in lightweight concrete at different concentrations (0.5, 1, 1.5, and 2.0 wt%) using two different methods including (i) addition and (ii) partial substitution for cement. Mechanical properties are determined by compressive strength tests. Chemical and morphological characterization is performed using scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. This study reveals that an increase in the percentage of ZnO nanoparticles as a substitute for cement directly decreases the compressive strength of lightweight concrete. For ZnO nanoparticles in the addition method, compressive strength is 10% lower than in the control specimens. However, the conclusions indicate constant compressive strength for all ZnO nanoparticle concentrations in the addition method.