bluuebunny/crossref_metadata_2025 huggingface dataset:
TP53 Co-Mutation Status Association with Clinical Outcomes in Patients with EGFR-Mutant Non-Small Cell Lung Cancer
Authors: Le Xiuning , Molife Cliff , Leusch Mark S. , Rizzo Maria Teresa , Peterson Patrick M. , Caria Nicola , Chen Yongmei , Gugel Elena Gonzalez , Visseren-Grul Carla
Published: December 2022

TP53 co-mutations have shown association with poor prognosis in various solid tumors. For EGFR-mutated advanced non-small cell lung cancer (aNSCLC), conflicting results exist regarding its impact on survival. Clinical outcomes and genomic data were obtained retrospectively from the real-world (rw) de-identified clinicogenomic database. Patients who initiated therapy for EGFR-mutated aNSCLC between January 2014 and December 2020 were identified. Clinical outcomes were evaluated by TP53-mutational status. In 356 eligible EGFR-mutated aNSCLC patients (median age 68 years), 210 (59.0%) had TP53 co-mutation and 146 (41.0%) had TP53 wild-type tumor. Unadjusted analysis showed significantly shorter survival in patients with TP53 co-mutation versus TP53 wild-type (rw progression-free survival [rwPFS]: HR = 1.4, 95% CI 1.1–1.9, p = 0.0196; overall survival [OS]: HR = 1.6, 95% CI 1.1–2.2, p = 0.0088). Multivariable analysis confirmed independent association between TP53 co-mutation and worse rwPFS (HR = 1.4, 95% CI 1.0–0.9, p = 0.0280) and OS (HR = 1.4, 95% CI 1.0–2.0, p = 0.0345). Directionally consistent findings were observed for response rates, and subgroups by EGFR-activating mutation and first-line (1 L) therapy, with more pronounced negative effect in 1 L EGFR-TKI subgroup. TP53 co-mutations negatively affected survival in patients with EGFR-mutated aNSCLC receiving standard 1 L therapy in real-world practice.
Incidence and Clinical Description of Lymphomas in Children and Adolescents with Vertical Transmission of HIV in Rio de Janeiro, Brazil, in Pre- and Post-Combined Antiretroviral Therapy Eras: A Multicentric Hospital-Based Survival Analysis Study
Authors: Duarte Nathalia Lopez , Bueno Ana Paula Silva , Sanches Bárbara Sarni , Ramos Gabriella Alves , Santos Julia Maria Bispo dos , Silva Henrique Floriano Hess e , de Oliveira Pondé Janaina , de Sá José Gilberto , Rossi Priscila Mazucanti , Horn Patricia Regina Cavalcanti Barbosa , das Neves Sztajnbok Denise Cardoso , de Paula Motta Rubini Norma , Milito Cristiane Bedran , de Abreu Thalita Fernandes , Land Marcelo Gerardin Poirot
Published: December 2022

The incidence of cancer in children living with HIV (CLWH) is high and lymphomas are the most common type of cancer in this population. The combined antiretroviral therapy (cART) changed the natural history of HIV infection. To determine the incidence and profile of these CLWH malignancies in Rio de Janeiro (RJ), Brazil, we conducted a retrospective and observational study of vertically infected CLWH, ranging from 0–20 incomplete years, from 1995 to 2018, at five reference centers. The study period was divided into three eras in accordance with the widespread use of cART in Brazil. 1306 patients were included. Of the 25 lymphomas found, 19 were AIDS-defining malignancies (ADM); 6 were non-AIDS-defining malignancies (NADM). The incidence rate (IR) of lymphoma developing was 1.70 per 1000 children-year (95% CI 1.09–2.50). ADM development IR decreased from 2.09–1.75–0.19 per 1000 children-year (p < 0.001) through cART eras. Cumulative Nelson–Aalen hazards of developing ADM over a 20-year period were 3.73% in the Early-cART era, 3.07% in the Mid-cART era, and 0.32% in the Late-cART era (p = 0.013). This study demonstrates the IR of lymphoma in CLWH in RJ, Brazil, as well as the benefit of cART in reducing ADM and death occurrence in the Post-cART era.
Needle-Tract Seeding of Pancreatic Cancer after EUS-FNA: A Systematic Review of Case Reports and Discussion of Management
Authors: Archibugi Livia , Ponz de Leon Pisani Ruggero , Petrone Maria Chiara , Balzano Gianpaolo , Falconi Massimo , Doglioni Claudio , Capurso Gabriele , Arcidiacono Paolo Giorgio
Published: December 2022

Needle-tract seeding (NTS) has been sporadically reported as complication of Endoscopic Ultrasound (EUS)-guided aspiration (FNA) in pancreatic adenocarcinoma (PDAC). However, the evidence of its treatment and outcome is sparse. Adhering to PRISMA guidelines, we conducted a systematic review of EUS-FNA NTS cases of PDAC and analyzed their management and outcome. Up to September 2022, the search query retrieved forty-five cases plus an unpublished case from our center, for a total of forty-six; 43.6% were male, with a mean age of 68.6 years. Thirty-four patients (87.1%) underwent an initial surgical resection, with only 44.1% and 5.9% undergoing adjuvant and neoadjuvant chemotherapy, respectively, and 5.9% undergoing both. The NTS nodule was mostly located in the posterior gastric wall, developing at a median of 19 months after primary resection; 82.4% underwent surgical resection of the seeding, while for 17.6%, palliative chemotherapy treatment. Follow-up after NTS diagnosis and treatment was reported for only twenty-three patients: when NTS was treated with surgery, the median overall survival was 26.5 months compared to 15.5 if treated with radio/chemotherapy. NTS after EUS-FNA of PDAC occurs late and might be treated aggressively with good results. Interestingly, only a low number of patients developing NTS underwent chemotherapy for the primary cancer, suggesting its possible protective role.
A Novel Therapeutic Target for Small-Cell Lung Cancer: Tumor-Associated Repair-like Schwann Cells
Authors: Cao Shuhui , Wang Yue , Zhou Yan , Zhang Yao , Ling Xuxinyi , Zhang Lincheng , Li Jingwen , Yang Yu , Wang Weimin , Shurin Michael R. , Zhong Hua
Published: December 2022

Small-cell lung cancer (SCLC), representing 15–20% of all lung cancers, is an aggressive malignancy with a distinct natural history, poor prognosis, and limited treatment options. We have previously identified Schwann cells (SCs), the main glial cells of the peripheral nervous system, in tumor tissues and demonstrated that they may support tumor spreading and metastasis formation in the in vitro and in vivo models. However, the role of SCs in the progression of SCLC has not been investigated. To clarify this issue, the cell proliferation assay, the annexin V apoptosis assay, and the transwell migration and invasion assay were conducted to elucidate the roles in SCLC of tumor-associated SCs (TA-SCs) in the proliferation, apoptosis, migration, and invasion of SCLC cells in vitro, compared to control group. In addition, the animal models to assess SC action’s effects on SCLC in vivo were also developed. The result confirmed that TA-SCs have a well-established and significant role in facilitating SCLC cell cancer migration and invasion of SCLC in vitro, and we also observed that SC promotes tumor growth of SCLC in vivo and that TA-SCs exhibited an advantage and show a repair-like phenotype, which allowed defining them as tumor-associated repair SCs (TAR-SCs). Potential molecular mechanisms of pro-tumorigenic activity of TAR-SCs were investigated by the screening of differentially expressed genes and constructing networks of messenger-, micro-, and long- non-coding RNA (mRNA-miRNA-lncRNA) using DMS114 cells, a human SCLC, stimulated with media from DMS114-activated SCs, non-stimulated SCs, and appropriate controls. This study improves our understanding of how SCs, especially tumor-activated SCs, may promote SCLC progression. Our results highlight a new functional phenotype of SCs in cancer and bring new insights into the characterization of the nervous system-tumor crosstalk.
Sialyltransferase Inhibitor Ac53FaxNeu5Ac Reverts the Malignant Phenotype of Pancreatic Cancer Cells, and Reduces Tumor Volume and Favors T-Cell Infiltrates in Mice
Authors: Miró Laura , López Júlia , Guerrero Pedro E. , Martínez-Bosch Neus , Manero-Rupérez Noemí , Moreno Mireia , Ortiz M. Rosa , Llop Esther , Navarro Pilar , Peracaula Rosa
Published: December 2022

Hypersialylation is a feature of pancreatic ductal adenocarcinoma (PDA) and it has been related to tumor malignancy and immune suppression. In this work, we have evaluated the potential of the sialyltransferase inhibitor, Ac53FaxNeu5Ac, to decrease tumor sialoglycans in PDA and to revert its malignant phenotype. Sialoglycans on PDA cells were evaluated by flow cytometry, and the functional impact of Ac53FaxNeu5Ac was assessed using E-selectin adhesion, migration, and invasion assays. PDA tumors were generated in syngeneic mice from KC cells and treated with Ac53FaxNeu5Ac to evaluate tumor growth, mice survival, and its impact on blocking sialic acid (SA) and on the tumor immune component. Ac53FaxNeu5Ac treatment on human PDA cells decreased α2,3-SA and sialyl-Lewisx, which resulted in a reduction in their E-selectin adhesion, and in their migratory and invasive capabilities. Subcutaneous murine tumors treated with Ac53FaxNeu5Ac reduced their volume, their SA expression, and modified their immune component, with an increase in CD8+ T-lymphocytes and NK cells. In conclusion, Ac53FaxNeu5Ac treatment weakened PDA cells’ malignant phenotype, thereby reducing tumor growth while favoring anti-tumor immune surveillance. Altogether, these results show the positive impact of reducing SA expression by inhibiting cell sialyltransferases and open the way to use sialyltransferase inhibitors to target this dismal disease.
A Multicenter Retrospective Cohort Study on Superior Vena Cava Resection in Non-Small-Cell Lung Cancer Surgery
Authors: Dell’Amore Andrea , Campisi Alessio , Bertolaccini Luca , Chen Chunji , Gabryel Piotr , Ji Chunyu , Piwkowski Cezary , Spaggiari Lorenzo , Fang Wentao , Rea Federico
Published: December 2022

Background: Surgery for non-small-cell lung cancers (NSCLCs) invading the superior vena cava (SVC) is rarely performed due to surgical complexities and reported poor prognoses. Different methods have been described to reconstruct the SVC, such as direct suture, patch use or prosthesis, according to its circumferential involvement. The aim of our study was to analyze the short- and long-term results of different types of SVC resection and reconstruction for T4 NSCLCs. Methods: Between January 2000 and December 2019, 80 patients received an anatomical lung resection with SVC surgery in this multicenter retrospective study. The partial resection and direct suture or patch reconstruction group included 64 patients, while the complete resection and prosthesis reconstruction group included 16 patients. The primary endpoints were as follows: long-term survival and disease-free survival. The secondary endpoints were as follows: perioperative complications and 30- and 90-day mortality. Unpaired t-tests or Mann–Whitney U tests for non-parametric variables were applied to discrete or continuous data, and the chi-square test was applied to dichotomous or categorical data. Survival rates were calculated using the Kaplan–Meier method and compared using the log-rank test. Results: No differences were found between the two groups in terms of general characteristics and surgical, oncological and survival outcomes. In particular, there were no differences in terms of early (50.0% vs. 68.8%, p = 0.178) and late complication frequency (12.5% vs. 12.5%, p = 1.000), 30- and 90-day mortality, R status, recurrence, overall survival (33.89 ± 40.35 vs. 35.70 ± 51.43 months, p = 0.432) and disease-free survival (27.56 ± 40.36 vs. 31.28 ± 53.08 months, p = 0.668). The multivariate analysis demonstrated that age was the only independent predictive factor for overall survival. Conclusions: According to our results, SVC resection has good oncological and survival outcomes, regardless of the proportion of circumferential involvement and the type of reconstruction.
Therapeutic Targeting of MERTK and BCL-2 in T-Cell and Early T-Precursor Acute Lymphoblastic Leukemia
Authors: Summers Ryan J. , Jain Juhi , Vasileiadi Eleana , Smith Brittany , Chimenti Madison L. , Yeung Tsz Y. , Kelvin James , Wang Xiaodong , Frye Stephen V. , Earp H. Shelton , Tyner Jeffrey W. , Dreaden Erik C. , DeRyckere Deborah , Graham Douglas K.
Published: December 2022

T-cell acute lymphoblastic leukemia (T-ALL) accounts for 15% of childhood ALL. The early T-precursor (ETP-ALL) subset is characterized by an immature T-cell phenotype, chemoresistance, and high rates of induction failure. MERTK receptor tyrosine kinase is ectopically expressed in half of T-ALLs, particularly those with an immature T-cell phenotype, suggesting a role in ETP-ALL. The anti-apoptotic protein B-cell lymphoma-2 (BCL-2) is essential for ETP-ALL cell survival. Here, we show that MERTK and BCL-2 mRNA and protein are preferentially expressed in ETP-ALL patient samples. The dual MERTK/FLT3 inhibitor MRX-2843 decreased MERTK activation and downstream signaling, inhibited cell expansion, and induced cell death in ETP-ALL cell lines. Further, 54% (21/39) of primary T-ALL patient samples were sensitive to MERTK inhibition. Treatment with MRX-2843 significantly reduced leukemia burden and prolonged survival in cell-line-derived T-ALL and ETP-ALL xenograft models. In a patient-derived ETP-ALL xenograft model, treatment with MRX-2843 markedly reduced peripheral blood leukemia and spleen weight compared to vehicle-treated mice and prolonged survival. MRX-2843 also synergized with venetoclax to provide enhanced anti-leukemia activity in ETP-ALL cell cultures, with a dose ratio of 1:20 MRX-2843:venetoclax providing optimal synergy. These data demonstrate the therapeutic potential of MRX-2843 in patients with T-ALL and provide rationale for clinical development. MRX-2843 monotherapy is currently being tested in patients with relapsed leukemia (NCT04872478). Further, our data indicate that combined MERTK and BCL-2 inhibition may be particularly effective for treatment of ETP-ALL.
Assembled Porphyrin Nanofiber on the Surface of g-C3N4 Nanomaterials for Enhanced Photocatalytic Degradation of Organic Dyes
Authors: Lai Hoan Thi , Nguyen Giang Thi , Tran Nga Thuy , Nguyen Thanh Tung , Van Tran Chinh , Nguyen Duy Khiem , Chang S. W. , Chung W. Jin , Nguyen Dinh Duc , Thi Hoai Phuong Nguyen , La Duong Duc
Published: December 2022

In this work, a g-C3N4/porphyrin nanocomposite was fabricated through the self-assembling of monomeric Tetrakis (4-carboxyphenyl) porphyrin (TCPP) molecules with g-C3N4 nanomaterials. The characterizing results showed a good distribution of TCPP nanofibers with a diameter of < 100 nm and several micrometers in length on the g-C3N4 nanoflakes’ surfaces. The prepared g-C3N4/porphyrin nanocomposite had two bandgap energies of 2.38 and 2.7 eV, which could harvest a wide range of photon energy in the light spectrum, particularly in visible light. The obtained C3N4/TCPP nanocomposite revealed a remarkable photodegradation efficiency toward rhodamine B dyes, with a RhB removing rate of 3.3 × 10−2 min−1. The plausible mechanism for the photocatalytic performance of the g-C3N4/porphyrin photocatalyst for the RhB dye’s degradation was also studied and discussed.
Synergistic Effect of Amorphous Ti(IV)-Hole and Ni(II)-Electron Cocatalysts for Enhanced Photocatalytic Performance of Bi2WO6
Authors: Sun Chenjing , Zhang Kaiqing , Wang Bingquan , Wang Rui
Published: December 2022

Bi2WO6 has become a common photocatalyst due to its advantages of simple synthesis and high activity. However, the defects of pure Bi2WO6 such as low light reception hinder its application in photocatalysis. In this study, based on the modification of Bi2WO6 with Ti(IV) as a cavity co-catalyst, new Ni- and Ti-doped nanosheets of Bi2WO6 (Ni/Ti-Bi2WO6) were prepared by a one-step wet thermal impregnation method and used for the photocatalytic degradation of tetracycline. The experimental results showed that the photocatalytic activity of Ni/Ti-Bi2WO6 modified by the two-component catalyst was significantly better than those of pure Bi2WO6 and Ti-Bi2WO6 modified with Ti(IV) only. The photocatalytic effect of Ni/Ti-Bi2WO6 with different Ni/Ti molar ratios was investigated by the degradation of TC. The results showed that 0.4Ni/Ti-Bi2WO6 possessed the best photocatalytic performance, with a degradation rate of 92.9% at 140 min TC. The results of cycling experiments showed that the catalyst exhibited high stability after five cycles. The scavenger experiment demonstrated that the h+ and O2− were the main reactive species. The enhanced photocatalytic activity of Bi2WO6 could be attributed to the synergistic effect between the Ti(IV) as a hole cocatalyst and Ni(II) as an electron cocatalyst, which effectively promoted the separation of photogenerated carriers.
Kinetic Mathematical Modeling of Oxidative Phosphorylation in Cardiomyocyte Mitochondria
Authors: Tseng Wen-Wei , Wei An-Chi
Published: December 2022

Oxidative phosphorylation (OXPHOS) is an oxygen-dependent process that consumes catabolized nutrients to produce adenosine triphosphate (ATP) to drive energy-dependent biological processes such as excitation-contraction coupling in cardiomyocytes. In addition to in vivo and in vitro experiments, in silico models are valuable for investigating the underlying mechanisms of OXPHOS and predicting its consequences in both physiological and pathological conditions. Here, we compare several prominent kinetic models of OXPHOS in cardiomyocytes. We examine how their mathematical expressions were derived, how their parameters were obtained, the conditions of their experimental counterparts, and the predictions they generated. We aim to explore the general landscape of energy production mechanisms in cardiomyocytes for future in silico models.
Boron Vehiculating Nanosystems for Neutron Capture Therapy in Cancer Treatment
Authors: Ailuno Giorgia , Balboni Alice , Caviglioli Gabriele , Lai Francesco , Barbieri Federica , Dellacasagrande Irene , Florio Tullio , Baldassari Sara
Published: December 2022

Boron neutron capture therapy is a low-invasive cancer therapy based on the neutron fission process that occurs upon thermal neutron irradiation of 10B-containing compounds; this process causes the release of alpha particles that selectively damage cancer cells. Although several clinical studies involving mercaptoundecahydro-closo-dodecaborate and the boronophenylalanine–fructose complex are currently ongoing, the success of this promising anticancer therapy is hampered by the lack of appropriate drug delivery systems to selectively carry therapeutic concentrations of boron atoms to cancer tissues, allowing prolonged boron retention therein and avoiding the damage of healthy tissues. To achieve these goals, numerous research groups have explored the possibility to formulate nanoparticulate systems for boron delivery. In this review. we report the newest developments on boron vehiculating drug delivery systems based on nanoparticles, distinguished on the basis of the type of carrier used, with a specific focus on the formulation aspects.
Mass Spectrometry as an Analytical Tool for Detection of Microplastics in the Environment
Authors: Chun Sechul , Muthu Manikandan , Gopal Judy
Published: December 2022

Plastic particles smaller than 5 mm accumulate in aqueous, terrestrial, and atmospheric environments and their discovery has been a serious concern when it comes to eco-toxicology and human health risk assessment. In the following review, the potential of mass spectrometry (MS) for the detection of microplastic (MP) pollutants has been elaborately reviewed. The use of various mass spectrometric techniques ranging from gas chromatography–mass spectrometry (GC-MS), liquid chromatographic mass spectrometric (LC-MS) to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), including their variants, have been reviewed. The lapses in the detection system have been addressed and future recommendations proposed. The challenges facing microplastics and their detection have been discussed and future directions, including mitigation methods, have been presented.
Time in Range in Children with Type 1 Diabetes before and during a Diabetes Camp—A Ceiling Effect?
Authors: Nagl Katrin , Bozic Ina , Berger Gabriele , Tauschmann Martin , Blauensteiner Nicole , Weimann Katharina , Mader Julia K. , Rami-Merhar Birgit
Published: December 2022

Background: The aim of this study was to assess and compare the time in range (TIR) of children with type 1 diabetes (T1D) before and during a diabetes summer camp using different therapy modalities. Methods: A retrospective analysis of continuous glucose monitoring (CGM) data collected from 26 children with T1D (mean age: 11.0 ± 1.4 years; 62% female; 62% on insulin pump; Hb1Ac 7.3 ± 0.8% (56.3 ± 8.7 mmol/mol) before and during a 14-day summer camp. CGM methods: 50% intermittently scanned CGM (isCGM) and 50% real-time CGM (rtCGM). No child was using a hybrid closed loop system. Results: Mean TIR during camp was significantly higher than before camp ((67.0 ± 10.7%) vs. 58.2% ± 17.4%, p = 0.004). There was a significant reduction in time above range (TAR) (p = 0.001) and increase in time below range (TBR) (p < 0.001), Children using isCGM showed a more pronounced improvement in TIR during camp compared to rtCGM-users (p = 0.025). The increase in TIR strongly correlated with numbers of scans per day in isCGM-users (r = 0.751, p = 0.003). Compared to isCGM-users, rtCGM-users showed significantly less TBR. The TIR target was met by 30.8% of participants during camp. Conclusion: Glycemic control improved significantly during the camp. However, on average, the therapy goal (TIR > 70%) could not be achieved despite great professional effort.
Lessons Learned from the Impact of HIV Status Disclosure to Children after First-Line Antiretroviral Treatment Failure in Kinshasa, DR Congo
Authors: Kitetele Faustin Nd. , Lelo Gilbert M. , Akele Cathy E. , Lelo Patricia V. M. , Aketi Loukia , Mafuta Eric M. , Tylleskär Thorkild , Kashala-Abotnes Espérance
Published: December 2022

HIV status disclosure to children remains a challenge in sub-Saharan Africa. For sociocultural reasons, parents often delay disclosure with subsequent risks to treatment compliance and the child’s psychological well-being. This article assesses the effects of HIV disclosure on second-line ART compliance after first-line failure. We conducted a retrospective study of 52 HIV-positive children at Kalembelembe Pediatric Hospital in Kinshasa who were unaware of their HIV status and had failed to respond to the first-line ART. Before starting second-line ART, some parents agreed to disclosure. All children were followed before and during the second-line ART. Conventional usual descriptive statistics were used. For analysis, the children were divided into two groups: disclosed to (n = 39) and not disclosed to (n = 13). Before starting the second-line ART, there was no difference in CD4 count between the two groups (p = 0.28). At the end of the first year of second-line ART, the difference was statistically significant between the two groups with regard to CD4% (p < 0.001) and deaths (p = 0.001). The children disclosed to also reported fewer depressive symptoms post-disclosure and had three times fewer clinic visits. HIV status disclosure to children is an important determinant of ART compliance and a child’s psychological well-being.
Enhancing the Raveling Resistance of Polyurethane Mixture: From the Perspective of Polyurethane Adhesive
Authors: Gao Junfeng , Wang Hainian , Chen Jiakang , Tang Boming
Published: December 2022

Polyurethane mixture, made of waste rubber particle, aggregate, and polyurethane adhesive, has low raveling resistance which affects the durability of the mixture. The objective of this study is to enhance the raveling resistance of polyurethane mixture. The content of polyol in the hydroxyl component was determined by Fourier transform infrared spectroscopy. The suitable curing conditions for polyurethane adhesive to enhance the raveling resistance were selected by the orthogonal experiment and mechanical tests. The relationship of the raveling resistance with crosslink density and elastic modulus was tested and calculated through the wear test. The results showed that when the ratios of the isocyanate component to the hydroxyl component were 1:3.2, 1:6.3, and 1:9.5, respectively, the isocyanate component was excessive. The ranking of the significance of the influence factors, from high to low, was first the curing temperature, then curing time, and finally the blending ratio; within the ranges of blending ratio, curing temperature, and curing time selected in this study, the appropriate blending ratio was 10:2, and the curing time was 6 h. For the polyurethane mixture involved in this study to obtain high raveling resistance, if a crosslinking agent or a new polyurethane adhesive is added, the tensile strength and tensile elastic modulus should be in the range of 3.02 to 3.27 MPa and 5.50 to 6.02 MPa, respectively; when using the FS2 polyurethane adhesive directly, the suitable curing conditions for the mixture are 90 °C and 6 h or 80 °C and 6 h. The results from this study could be beneficial for obtaining a high raveling resistance for the polyurethane mixture.
Possibilities of Using the Duplex System Plasma Nitriding + CrN Coating for Special Components
Authors: Dobrocky David , Pokorny Zdenek , Vitek Roman , Prochazka Jiri , Studeny Zbynek , Joska Zdenek , Sedlak Josef , Slany Martin , Kolomy Stepan
Published: December 2022

The article deals with the replacement of hard chrome plating by applying the duplex system plasma nitriding + CrN coating (hereinafter referred to as PN + CrN). The goal of the research was to find a suitable alternative for steel surface treatment that would replace hard chrome plating and ensure similar mechanical and tribological properties. An exposed part of a small-bore weapon was selected for evaluation, namely the gas piston of the 42CrMo4 steel breech mechanism drive. The PN + CrN duplex system was compared with a hard chrome coating as well as a self-deposited CrN coating. The mentioned surface treatments were evaluated in terms of metallography, mechanical and tribological properties and surface texture. From the mechanical properties, the hardness of the surface was analyzed, an indentation test was performed (Mercedes test) and adhesive-cohesive behavior was evaluated (Scratch test). Furthermore, an instrumented penetration test was performed (an evaluation of plastic and elastic deformation work and indentation hardness). As part of the assessment of tribological properties, the Ball-on-Flat test, the measurement of the coefficient of friction and the measurement of traces of wear were performed. The surface texture was evaluated in terms of morphology and surface roughness measurement by selected 2D and 3D parameters. The PN + CrN duplex system showed higher hardness than hard chrome, better tribological properties (lower friction coefficient), but worse surface texture. The PN + CrN duplex system has proven to be a suitable alternative to the hard chrome coating for exposed parts of small-caliber weapons, which can be applied in arms production.
Review on the Antioxidant Activity of Phenolics in o/w Emulsions along with the Impact of a Few Important Factors on Their Interfacial Behaviour
Authors: Kiokias Sotirios , Oreopoulou Vassiliki
Published: December 2022

This review paper focuses on the antioxidant properties of phenolic compounds in oil in water (o/w) emulsion systems. The authors first provide an overview of the most recent studies on the activity of common, naturally occurring phenolic compounds against the oxidative deterioration of o/w emulsions. A screening of the latest literature was subsequently performed with the aim to elucidate how specific parameters (polarity, pH, emulsifiers, and synergistic action) affect the phenolic interfacial distribution, which in turn determines their antioxidant potential in food emulsion systems. An understanding of the interfacial activity of phenolic antioxidants could be of interest to food scientists working on the development of novel food products enriched with functional ingredients. It would also provide further insight to health scientists exploring the potentially beneficial properties of phenolic antioxidants against the oxidative damage of amphiphilic biological membranes (which link to serious pathologic conditions).
Green Synthesis of ZnO Nanostructures Using Pyrus pyrifolia: Antimicrobial, Photocatalytic and Dielectric Properties
Authors: Ali Zainal Abidin , Shudirman Iqabiha , Yahya Rosiyah , Venkatraman Gopinath , Hirad Abdurahman Hajinur , Ansari Siddique Akber
Published: December 2022

In this study, zinc oxide nanostructures (ZnO NS) were synthesized using Pyrus pyrifolia fruit extract. Biophysical characterization results confirmed that the synthesized materials are crystalline wurtzite ZnO structures. Field emission scanning electron microscopy (FESEM) revealed that the ZnO NS are cubical, and the sizes range 20–80 nm. Transmission electron microscopy (TEM) and XRD results revealed a crystal lattice spacing of 0.23 nm and (101) the crystalline plane on ZnO NS. UV-Visible spectrophotometer results showed an absorbance peak at 373 nm. The ZnO NS demonstrated significant antibacterial activity analyzed by metabolic activity analysis and disc diffusion assay against Escherichia coli and Staphylococcus aureus. FESEM analysis confirmed the bacterial membrane disruption and the release of cytoplasmic contents was studied by electron microscopy analysis. Further, ZnO NS achieved good photocatalytic activity of decolorizing 88% of methylene blue (MB) in 60 min. The dielectric constant and loss of ZnO were found to be 3.19 and 2.80 at 1 kHz, respectively. The research findings from this study could offer new insights for developing potential antibacterial and photocatalytic materials.
A Survey-Based Study on Physical Activity Promotion for Individuals with a Current or Past Diagnosis of Cancer in Canada
Authors: Smith-Turchyn Jenna , Sabiston Catherine M. , Ball Elizabeth , Mukherjee Som D.
Published: December 2022

Purpose: To determine the prevalence and content of discussions regarding physical activity (PA) promotion between individuals with a current or past diagnosis of cancer and their oncology care team. Methods: Design and Procedure: A cross-sectional survey on PA discussion between individuals with a current or past diagnosis of cancer and their oncology care team was conducted at a single timepoint. Participants: Eligible participants were adults with a current or past diagnosis of cancer at any time point in their cancer treatment who had a pre-scheduled appointment with their oncology care team. Results: A total of 100 participants completed the survey. PA-related discussions happened in 41% of the patient-provider interactions and 66% of respondents reported PA discussions at some point during care. No significant association occurred between cancer type, stage, or treatment status and PA discussions at any timepoint (all p’s > 0.05). Most respondents were satisfied with the education provided on PA (54%); however, only 37% were sufficiently active. Those receiving education from their medical oncologist were more likely to be ‘sufficiently active’ (p = 0.020) according to the Godin Leisure Time Exercise Questionnaire. Conclusions: Most respondents discuss PA with an oncology care provider at some point during their cancer treatment; however, few are sufficiently active. Future research is needed to determine strategies to facilitate PA promotion and close the gap between discussions and actual physical activity behavior.
Molecular Landscape of Pediatric Thyroid Cancer: A Review
Authors: Guleria Prerna , Srinivasan Radhika , Rana Chanchal , Agarwal Shipra
Published: December 2022

Thyroid carcinomas (TC) are rare in the pediatric population; however, they constitute the most common endocrine malignancy. Despite some similarities with adult carcinomas, they have distinct clinical behavior and responses to therapy due to their unique pathology and molecular characteristics. The age cut-off used for defining the pediatric age group has been variable across different studies, and the universally accepted recommendations influence accurate interpretation of the available data. Moreover, factors such as radiation exposure and germline mutations have greater impact in children than in adults. Papillary TC is the most common and the most evaluated pediatric TC. Others, including follicular, poorly differentiated and medullary carcinomas, are rarer and have limited available literature. Most studies are from the West. Asian studies are primarily from Japan, with few from China, India, Saudi Arabia and Republic of Korea. This review provides a comprehensive account of the well-established and novel biomarkers in the field, including point mutations, fusions, miRNA, and thyroid differentiation genes. Familial and syndromic associations are also discussed. Current management guidelines for pediatric patients are largely derived from those for adults. An awareness of the molecular landscape is essential to acknowledge the uniqueness of these tumors and establish specific diagnostic and therapeutic guidelines.
Do Import Tariff Adjustments Bolster Domestic Production? Analysis of the South African-Brazilian Poultry Market Case
Authors: Nkgadima Kgothatso , Muchopa Chiedza L.
Published: December 2022

The South African poultry industry plays an important food security contributory role, hence, consumer and producer interests ought to be accounted for in order to maintain the long term value of the industry in the presence of import competition. This study used the Error Correction Model (ECM) to investigate the relationship between the variables as well as the Impulse Response Model to assess the level of responsiveness of import volume and domestic production due to changes in the import tariff for the period 2010m04 to 2020m06. The ECM results with a negative ECT value of −0.53, indicate that a long run relationship exists between domestic broiler production volume, imported broiler quantity and the Ad Valorem import tariff. The Impulse Response Model has shown that over ten periods, the adjustments in the Ad Valorem tariff initially produce desirable results showing that a one standard deviation shock to the Ad Valorem tariff culminates in a sharp decline in import volume and a sharp increase in domestic volume produced. However, this response cannot be maintained over the long term, thus signaling the need for a more effective and viable solution other than an increase in the Ad Valorem import tariff to alleviate the stiff competition between domestic production and an increased supply of broiler imports. The study findings have policy implications for resolving internal issues in the South African poultry industry, such as improving production capacity and sector competitiveness at the same time maintaining the gains from trade especially for consumers.
The Role of Social Capital Facing Pandemic COVID-19 in Tourism Village to Support Sustainable Agriculture (Empirical Evidence from Two Tourism Villages in Indonesia)
Authors: Auliah Aidha , Prayitno Gunawan , Ari Ismu Rini Dwi , Rahmawati  , Wardani Lusyana Eka , Meidiana Christia
Published: December 2022

Pujon Kidul and Bangelan villages (Indonesia) are two tourist villages that have succeeded in developing agricultural potential associated with tourism activities. The characteristics of Pujon Kidul Village and Bangelan Village refer to the tourism activities in the villages. This study aims to find out how community social capital can influence collective action in dealing with the COVID-19 pandemic in Pujon Kidul Tourism Village and Bangelan Tourism Village. This study uses the structural equation model (SEM) method, which is applied to the SEM, PLS (Partial Least Square) and AMOS (Analysis of Moment Structures) software to get complete results. The results of the analysis, Bangelan Village and Pujon Kidul Village are models that can survive in a pandemic crisis situation. The results of the model show that social capital in dealing with a pandemic in Bangelan Village is most influenced by trust in the government, while in Pujon Kidul Village it is most influenced by trust among fellow communities. The existence of social capital plays a role in decision-making on collective actions to deal with pandemics in tourist villages. Thus, by knowing how the community is recovering during the COVID-19 pandemic, businesses can run smoothly and according to government policy.
State-of-the-Art Review of the Key Factors Affecting Electric Vehicle Adoption by Consumers
Authors: Anastasiadou Konstantina , Gavanas Nikolaos
Published: December 2022

The dependence of road transport on fossil fuels and its contribution to greenhouse gas (GHG) and pollutant emissions are main concerns leading to the need for shifting toward alternative energy sources and, namely, electromobility. The current paper aims to identify the key determinants affecting the consumer adoption of electric vehicles (EVs), focusing on private passenger cars. Toward this purpose, a systematic review of recent international literature is conducted in order to identify motivators and barriers, which are then categorized following the PESTLE (Political–Economic–Social–Technological–Legal–Environmental) approach. Based on the review results, main policy implications and recommendations are discussed. A main conclusion is that the recent literature highlights a wide array of determinants, without converging as to which ones are the most influential regarding EV adoption by consumers. Another conclusion is that the environmental aspects are less important for consumers than anticipated, despite the concerns about climate change and renewable energy transition.
Applying Digital Image Correlation (DIC) Techniques to Characterise Plywood According to UNE-EN 314 Standards: Bonding Quality Tests
Authors: Fernández Zúñiga Elena , García Esteban Luis , García Fernández Francisco , de Palacios Paloma , García-Iruela Alberto , García Ismael , Lozano Miguel , Fernández Canteli Alfonso
Published: December 2022

The determination of plywood bonding quality by shear testing is one of the most commonly used and informative tests, alongside bending strength, to characterise this compound material. Two types of birch plywood were studied: indoor use (class 1), thickness 18 mm, urea-formaldehyde glue; and exposed outdoor use (class 3), thickness 15 mm, phenolic formaldehyde glue. The corresponding pre-treatments indicated in standards UNE-EN 314-1 and -2 were applied to each type. To test the suitability of the method, the technique of digital image correlation (DIC) was used to obtain information about the complex modes of fracture present in compound materials such as plywood. Assessing the results based on the statistical extreme value theory provided more reliable and robust information about the failure strength values for low failure probabilities. The results confirm that DIC can be used to determine the true distribution of the deformations during the fracture process and, therefore, it provides greater knowledge of the details of the failure process. Statistical evaluation of the experimental data obtained with DIC indicates that test results exceeding the time limit of 30 ± 10 s should be evaluated, contrary to the operating procedure in standard UNE-EN 314-1, which states that they are invalid.
Joint Random Forest and Particle Swarm Optimization for Predictive Pathloss Modeling of Wireless Signals from Cellular Networks
Authors: Omasheye Okiemute Roberts , Azi Samuel , Isabona Joseph , Imoize Agbotiname Lucky , Li Chun-Ta , Lee Cheng-Chi
Published: December 2022

The accurate and reliable predictive estimation of signal attenuation loss is of prime importance in radio resource management. During wireless network design and planning, a reliable path loss model is required for optimal predictive estimation of the received signal strength, coverage, quality, and signal interference-to-noise ratio. A set of trees (100) on the target measured data was employed to determine the most informative and important subset of features, which were in turn employed as input data to the Particle Swarm (PS) model for predictive path loss analysis. The proposed Random Forest (RF-PS) based model exhibited optimal precision performance in the real-time prognostic analysis of measured path loss over operational 4G LTE networks in Nigeria. The relative performance of the proposed RF-PS model was compared to the standard PS and hybrid radial basis function-particle swarm optimization (RBF-PS) algorithm for benchmarking. Generally, results indicate that the proposed RF-PS model gave better prediction accuracy than the standard PS and RBF-PS models across the investigated environments. The projected hybrid model would find useful applications in path loss modeling in related wireless propagation environments.
Simulated Digestion and Fermentation In Vitro by Obese Human Gut Microbiota of Sulforaphane from Broccoli Seeds
Authors: Sun Yifei , Tang Zhaocheng , Hao Tingting , Qiu Zeyu , Zhang Baolong
Published: December 2022

Background: sulforaphane is a kind of isothiocyanate, which is obtained by hydrolysis of glucosinolate by the unique myrosinase in plants. It has been proved to prevent the occurrence of many chronic diseases, such as obesity, diabetes and cancer. Objective: The impact of SFN on obese human gut flora, however, has not been established. Methods: In this research, SFN was isolated from broccoli seeds and then refined to achieve 95% purity. Next, an investigation was conducted into the digestion and fermentation processes of SFN. Results: The stability of the SFN in simulated saliva, gastric fluid, and intestinal juice provides evidence that it can reach the gut and be available for utilization by gut microflora. In vitro fermentation of SFN by gut microbes in obese patients results in alteration in constitution of microbiota and production of short chain fatty acids. As the result of SFN ingestion by human gut bacteria, the content of butyric and valeric acids increased 1.21- and 1.46-fold, respectively. In obese human guts, the relative abundances of the beneficial genera including Lactobacillus, Weissella, Leuconosto, Algiphilus and Faecalibacterium significantly increased, whilst the detrimental genera, such as Escherichia-Shigella, Klebsiella, Clostridium_sensu_stricto_1, Sutterella, Megamonas and Proteus drastically declined. Conclusion: Taken together, these findings demonstrate that SFN can be used as a nutraceutical ingredient for obese patients and for improving human health.
Semi-Synthetic Click-Gelatin Hydrogels as Tunable Platforms for 3D Cancer Cell Culture
Authors: Hipwood Luke , Clegg Julien , Weekes Angus , Davern Jordan W. , Dargaville Tim R. , Meinert Christoph , Bock Nathalie
Published: December 2022

Basement membrane extracts (BME) derived from Engelbreth–Holm–Swarm (EHS) mouse sarcomas such as Matrigel® remain the gold standard extracellular matrix (ECM) for three-dimensional (3D) cell culture in cancer research. Yet, BMEs suffer from substantial batch-to-batch variation, ill-defined composition, and lack the ability for physichochemical manipulation. Here, we developed a novel 3D cell culture system based on thiolated gelatin (Gel-SH), an inexpensive and highly controlled raw material capable of forming hydrogels with a high level of biophysical control and cell-instructive bioactivity. We demonstrate the successful thiolation of gelatin raw materials to enable rapid covalent crosslinking upon mixing with a synthetic poly(ethylene glycol) (PEG)-based crosslinker. The mechanical properties of the resulting gelatin-based hydrogels were readily tuned by varying precursor material concentrations, with Young’s moduli ranging from ~2.5 to 5.8 kPa. All hydrogels of varying stiffnesses supported the viability and proliferation of MDA-MB-231 and MCF-7 breast cancer cell lines for 14 and 21 days of cell culture, respectively. Additionally, the gelatin-based hydrogels supported the growth, viability, and osteogenic differentiation of patient-derived preosteoblasts over 28 days of culture. Collectively, our data demonstrate that gelatin-based biomaterials provide an inexpensive and tunable 3D cell culture platform that may overcome the limitations of traditional BMEs.
Pre-Existing Diabetes Mellitus, Hypertension and KidneyDisease as Risk Factors of Pre-Eclampsia: A Disease of Theories and Its Association with Genetic Polymorphism
Authors: Alanazi Abdullah Salah , Victor Francis , Rehman Kanwal , Khan Yusra Habib , Yunusa Ismaeel , Alzarea Abdulaziz Ibrahim , Akash Muhammad Sajid Hamid , Mallhi Tauqeer Hussain
Published: December 2022

Pre-existing diabetes, hypertension and kidney disorders are prominent risk factors of pre-eclampsia (PE). It is a multifactorial pregnancy disorder associated with high blood pressure, proteinuria, and multiorgan failure, which develops after the 20th week of pregnancy. It is one of the most feared pregnancy disorders, as it consumes thousands of fetomaternal lives per annum. According to clinical and pathological studies, the placenta appears to be a key player in the pathogenesis of PE; however, the exact origin of this disorder is still under debate. Defective placentation and angiogenesis are the hallmarks of PE progression. This angiogenic imbalance, together with maternal susceptibility, might determine the severity and clinical presentation of PE. This article comprehensively examines the mechanisms of pathogenesis of PE and current evidence of the factors involved in its progression. Finally, this article will explore the genetic association of PE, various candidate genes, their proposed mechanisms and variants involved in its pathogenesis.
Short- and Long-Term Effects of Underemployment on Workers’ Health: Empirical Analysis from the China Labor Force Dynamics Survey
Authors: Li Nan , Liang Huanhuan , Gao Yi , Wu Dan
Published: December 2022

Underemployment is a global problem. This study aimed to assess the short- and long-term effects of underemployment (hidden unemployment) on workers’ health, using data from the China Labor-force Dynamic Survey (CLDS) 2016 and 2014. Indicators reflecting workers’ self-rated health, mental health, prevalence of illness over time, and employment status were analyzed using logit regression models, propensity score matching methods, and instrumental variable methods. Empirical analyses showed that: (1) In the short-term, the impact on health is multidimensional, with underemployment significantly associated with a decline in workers’ self-rated health, an increase in the propensity for depression, and an increase in the prevalence of illness over a certain period of time. (2) In the long-term, the experience of underemployment two years in the past is associated with a current decline in workers’ mental health. That is, the negative effects of underemployment on workers’ mental health persist and do not disappear rapidly over time. The results demonstrated that underemployment is detrimental to workers’ health in the short- and long-term. In the context of epidemic prevention and control, the government and society should focus on this expanding group, establish labor protection mechanisms, and reduce the multiple effects of underemployment on workers’ health.