bluuebunny/crossref_metadata_2025 huggingface dataset:
Varietal Discrimination of Trebbiano d’Abruzzo, Pecorino and Passerina White Wines Produced in Abruzzo (Italy) by Sensory Analysis and Multi-Block Classification Based on Volatiles, Organic Acids, Polyphenols, and Major Elements
Authors: Biancolillo Alessandra , D’Archivio Angelo Antonio , Pietrangeli Fabio , Cesarone Gaia , Ruggieri Fabrizio , Foschi Martina , Reale Samantha , Rossi Leucio , Crucianelli Marcello
Published: September 2022

Reliable analytical methods able to establish wine authenticity and compliance with the origin/variety denomination are essential tools for the safeguarding of consumers from fraud. In this work, we attempted the discrimination of certified monovarietal white wines produced in the Abruzzo region (Central Italy) in 2015 with Trebbiano d’Abruzzo, Pecorino or Passerina grapes, all belonging to the Trebbiano variety. A preliminary sensory analysis revealed a high similarity among the three wines. The aroma profile and polyphenol and organic acid profiles were collected by gas chromatography and ultra-high-performance liquid chromatography, respectively, on 46 samples representing the three wine varieties. Eventually, the concentration of 14 elements in the same samples, determined by inductively coupled plasma optical emission spectrometry, was considered. Partial Least Squares Discriminant Analysis pursued on the individual analytical responses gave unsatisfactory results in terms of varietal discrimination. A data fusion approach, Sequential and Orthogonalized Partial Least Squares Linear Discriminant Analysis, on the other hand, provided better results as it misclassified only three (out of eighteen) external samples. Tartaric acid, malic acid, Cu, Na, Ni, Sr, Ca, Fe, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl hexanoate, and 2-phenylethyl acetate were found to be the variables relevant in the discrimination of the three monovarietal wines.
Piezoelectric-Based Sensor Concept and Design with Machine Learning-Enabled Using COMSOL Multiphysics
Authors: Mourched Bachar , Hoxha Mario , Abdelgalil Ahmed , Ferko Ndricim , Abdallah Mariam , Potams Albert , Lushi Ardit , Turan Halil Ibrahim , Vrtagic Sabahudin
Published: September 2022

This paper presents the concept and design of a system that embeds piezoelectric sensors to measure the voltage of a mechanical load applied to it. COMSOL Multiphysics, a finite element simulation tool, was used to design the system and analyze the data to find a possible fingerprint of voltage changes. The sensors’ voltage readings were affected by the load applied to the surface of the structure with different magnitudes and speeds. The analyzed data show the effect of position and mass on the voltage readings and indicates the possibility of speed prediction. The obtained dataset results validated the concept of the proposed system, where the collected data can serve as a digital data pipeline model for future research on different artificial intelligence (AI) or machine learning (ML) modeling applications. From the obtained data, a reasonable view shows that voltage reading matrices can be utilized for the detection of vehicle speed, location, and mass if used as training data for machine learning modeling, which can benefit the Internet of Things (IoT) technology.
Sustainable Design and Management of Industrial Systems—A Human Factors Perspective
Authors: Coelho Denis Alves
Published: September 2022

The aim of this concept article is to articulate multiple contributions from socio-technical fields into an approach for sustaining human-centred lifecycle management of industrial systems. Widespread digitalization and advanced robotics have fostered interest on innovative human-machine integration and sophisticated organizational transformation that is conducive to meeting the challenges of sustainability. Complementing technology-driven and data-driven approaches to industrial systems development, the human factors approach offers a systems perspective that is at once human-centred while striving for overall system performance, by considering technological and organizational perspectives alike. The paper presents a set of recent human factors developments, selected based on their potential to advance sustainability in industrial systems, including an activity-centred design perspective of industrial systems, and a unified and entangled view on organizational goals yielding a dynamic change approach to socio-technical systems management. Moreover, developments in organizational resilience are coupled with recent breakthrough empirical understanding of conditions conducive to attaining resilience in operations. The cross-pollination of the human factors developments is further pursued, resulting in a proposal of combined key organizational vectors that can mutually leverage and sustain human-centred design and management of industrial systems (production and logistics systems alike) for resilience. Systems thinking encompassing human, organizational and technological perspectives supports integration of insights across entangled domains; this can leverage both system enhancements that promote the satisfaction of dynamic situation-dependent goals, as well as the fulfilment of objectives derived from long-term values of an organization.
Uncovering the Fresh Snowfall Microbiome and Its Chemical Characteristics with Backward Trajectories in Daejeon, the Republic of Korea
Authors: Hassan Zohaib Ul , Nirmalkar Jayant , Park Dongju , Jung Jinsang , Kim Seil
Published: September 2022

Snow covers a large surface area of the Earth and provides a surface for the exchange of biological and chemical components. However, the microbial composition and chemical components of snow are poorly understood. We assessed the bacterial and fungal diversity and chemical characteristics in freshly deposited snowfall samples collected from a sub-urban site in Daejeon, the Republic of Korea. We analyzed the snow samples using DNA amplification followed by Illumina MiSeq Sequencing for the microbiome, ion chromatography for the cations (Na+, Ca2+, Mg2+, and NH4+) and anions (SO42−, NO3−, and Cl−), and a water-soluble organic carbon (WSOC) and water-soluble nitrogen (WSTN) analyzer for WSOC and WSTN. NO3−, Actinobacteria (bacteria), and Ascomycota (fungi) were the most abundant components in the fresh snowfall samples. The air mass backward trajectories arrived mostly at this site from the northwest direction during this study period, which included the regions belonging to Russia, China, Mongolia, the Gobi Desert, the Yellow Sea, and South Korea. Principal component analysis suggested that the snow components were associated with sources belonging to secondary chemical compounds, dust, and sea salt during the study period.
Scientific Appraisal and Therapeutic Properties of Plants Utilized for Veterinary Care in Poonch District of Jammu and Kashmir, India
Authors: Wani Zishan Ahmad , Farooq Adil , Sarwar Sobia , Negi Vikram S. , Shah Ali Asghar , Singh Bikarma , Siddiqui Sazada , Pant Shreekar , Alghamdi Huda , Mustafa Mahmoud
Published: September 2022

The importance of traditional and indigenous knowledge is acknowledged on a worldwide scale for its coexistence principles and sustainable use techniques. In view of this, the present study is an attempt to document the ethno-veterinary plants used by the tribal communities of Western Himalaya. This study also provides the scientific validation of herbal medicines used in ethno-veterinary practices through a reverse pharmacological approach. A total of 59 informants were selected through a non-probability sampling method. Detailed information on the medicinal plants used in ethno-veterinary practices along with their habits and habitats, part/s used, remedy preparation methods, additives/ingredients used during preparation and administration, dosages administered, and route of administration was collected. Data was analyzed for the Relative Frequency of Citations (RFC), Use Values (UV), Informant Consensus Factor (ICF), and Jaccard Index (JI). Further, a reverse pharmacological approach was used for scientific validations of the documented herbal knowledge of plant species. During the study, 56 plant species belonging to 54 genera and 39 families were documented. Asteraceae was the dominant family followed by Lamiaceae, Amaranthaceae and Fabaceae. Life forms were dominated by herbaceous species and leaves were the most common plant parts used. The highest Relative Frequency of Citations (RFC) and Use Values (UV) were recorded for Brassica rapa L. (Brassicaceae). The Pearson correlation coefficient between RFC and UV shows a strong positive correlation between the proportion of uses of a plant species within a sample of informants and the number of times that a particular use of a plant species was mentioned by the informant. Studies of the biological activity of ethno-veterinary plants can provide clues of promising leads for the isolation and identification of useful compounds that may be developed into pharmaceuticals for human welfare.
Characteristics of SARS-CoV-2 Infection in an Actively Monitored Cohort of Patients with Lupus Nephritis
Authors: Obrișcă Bogdan , Vornicu Alexandra , Jurubiță Roxana , Mocanu Valentin , Dimofte George , Andronesi Andreea , Sorohan Bogdan , Achim Camelia , Micu Georgia , Bobeică Raluca , Dina Constantin , Ismail Gener
Published: September 2022

(1) Background: We sought to investigate the impact of the COVID-19 pandemic in patients with lupus nephritis (LN); (2) Methods: A total of 95 patients with LN actively monitored in our department between 26 February 2020, when the first case of COVID-19 was diagnosed in Romania, and 1 May 2021, were included in the study. Multivariate logistic regression analysis was performed to identify the independent risk factors for SARS-CoV-2 infection; (3) Results: A total of 15 patients (15.8%) had a confirmed SARS-CoV-2 infection during a total follow-up time of 105.9 patient-years (unadjusted incidence rate: 14.28 SARS-CoV-2 infections per 100 patient-years). Median time to SARS-CoV-2 infection was 9.3 months (IQR: 7.2–11.3). The majority of patients had a mild form of SARS-CoV-2 infection (73.3%), while the remaining had moderate forms. None of the patients had a severe infection or a SARS-CoV-2-related death. The most frequent symptom was fatigue (73.3%), followed by loss of taste/smell (53.3%) and fever (46.7%). Forty percent of those with SARS-CoV-2 infection were hospitalized for a median 11.5 days (IQR:3.75–14). In the multivariate logistic regression analysis, a current oral corticosteroid dose ≥ 15 mg/day was associated with a 7.69-fold higher risk (OR, 7.69; 95%, 1.3–45.46), while the use of hydroxychloroquine was associated with a 91% lower risk for a SARS-CoV-2 infection (OR, 0.09; 95%CI, 0.01–0.59). (4) Conclusions: Our study confirms that the SARS-CoV-2 infection-associated morbidity might only be moderately increased in patients with LN. The current oral corticosteroid dose was the only independent predictor of infection occurrence, while use of hydroxychloroquine was associated with a protective effect.
Electrochemical Microneedles: Innovative Instruments in Health Care
Authors: Liao Zhijun , Zhou Qian , Gao Bingbing
Published: September 2022

As a significant part of drug therapy, the mode of drug transport has attracted worldwide attention. Efficient drug delivery methods not only markedly improve the drug absorption rate, but also reduce the risk of infection. Recently, microneedles have combined the advantages of subcutaneous injection administration and transdermal patch administration, which is not only painless, but also has high drug absorption efficiency. In addition, microneedle-based electrochemical sensors have unique capabilities for continuous health state monitoring, playing a crucial role in the real-time monitoring of various patient physiological indicators. Therefore, they are commonly applied in both laboratories and hospitals. There are a variety of reports regarding electrochemical microneedles; however, the comprehensive introduction of new electrochemical microneedles is still rare. Herein, significant work on electrochemical microneedles over the past two years is summarized, and the main challenges faced by electrochemical microneedles and future development directions are proposed.
Dual-Color Fluorescent Hydrogel Microspheres Combined with Smartphones for Visual Detection of Lactate
Authors: Yang Sisi , Tang Ziwen , Tian Yilong , Ji Xinghu , Wang Fubing , Xie Conghua , He Zhike
Published: September 2022

Since it is difficult for human eyes to distinguish between two identical colors with only <15% variation in brightness, mono-color fluorescent hydrogel microspheres have some limitations in the detection of lactate. Herein, we prepared novel dual-color fluorescent hydrogel microspheres, which can achieve hue transformation. Microspheres were prepared by introducing a fluorescent nanoparticle as the reference signal while CdTe QDs were used as the response signal. We used smartphones with image processing software to collect and analyze data. In this way, the signal of lactate was converted to RGB (red, green, and blue) values, which can be quantitatively read. Within 10 to 1500 μM, the R/G values of the microspheres had a linear relationship with the logarithm of the lactate concentration. Moreover, color cards for lactate detection were prepared, from which the color change and concentration of lactate could be easily read by the naked eye. It is worth mentioning that this method was successfully applied to screen patients with hyperlactatemia.
In Silico Investigation of SNR and Dermis Sensitivity for Optimum Dual-Channel Near-Infrared Glucose Sensor Designs for Different Skin Colors
Authors: Althobaiti Murad
Published: September 2022

Diabetes is a serious health condition that requires patients to regularly monitor their blood glucose level, making the development of practical, compact, and non-invasive techniques essential. Optical glucose sensors—and, specifically, NIR sensors—have the advantages of being non-invasive, compact, inexpensive, and user-friendly devices. However, these sensors have low accuracy and are yet to be adopted by healthcare providers. In our previous work, we introduced a non-invasive dual-channel technique for NIR sensors, in which a long channel is utilized to measure the glucose level in the inner skin (dermis) layer, while a short channel is used to measure the noise signal of the superficial skin (epidermis) layer. In this work, we investigated the use of dual-NIR channels for patients with different skin colors (i.e., having different melanin concentrations). We also adopted a Monte Carlo simulation model that takes into consideration the differences between different skin layers, in terms of blood content, water content, melanin concentration in the epidermis layer, and skin optical proprieties. On the basis of the signal-to-noise ratio, as well as the sensitivities of both the epidermis and dermis layers, we suggest the selection of wavelengths and source-to-detector separation for optimal NIR channels under different skin melanin concentrations. This work facilitates the improved design of a compact and non-invasive NIR glucose sensor that can be utilized by patients with different skin colors.
The Incidence, Localization and Clinical Relevance of Arterial Fenestrations and Their Association to Brain Aneurysms: A Case–Control Study Based on the STROBE Guidelines
Authors: Czyżewski Wojciech , Hoffman Zofia , Szymoniuk Michał , Korulczyk Patrycja , Torres Kamil , Staśkiewicz Grzegorz
Published: September 2022

Background: Fenestrations are rare, but well-known, vascular variations of the cerebral arteries. They are mostly incidental, asymptomatic angiographic findings and might precipitate vascular lesions such as AVM, aneurysmal dilatation, or even ischemic symptoms. However, association between arterial fenestration and brain aneurysms has not been clearly established. Objective: To evaluate whether incidence of arterial fenestrations are associated with brain aneurysm development and investigate the prevalence and most-common localizations of arterial fenestrations of the human brain. Design: Case–control study. Setting: All patients examined by CT angiography in University Hospital No. 4 in Lublin from 2009 to 2019. Patients: Each patient showing at least one cerebral aneurysm was included in the case group and each patient without cerebral aneurysm on CT angiography was included in the control group. Measurements: CT angiography examinations were conducted using the standard protocol used in the 1st Department of Radiology, Medical University of Lublin, Poland. The database and statistical research were conducted by use of the Statistica software (ver. 13.3, Tibco Software Inc., Palo Alto, CA, USA). Results: A total of 6545 CTA examinations were included in the study. Most of the aneurysms were located on the MCA: 629 (38.59%), ICA: 466 (28.59%) and AComA: 192 (11.78%). Cerebral arterial fenestration showed a non-statistically significant elevated risk for brain aneurysms in the entire study population (OR: 1.157; 95% CI: 0.826–1.621; p = 0.39). Among 6545 cranial CTA examinations, cerebral vessel fenestration was found in 49 of them, which constituted 0.75%. The most common vascular fenestrations were those located in the ACA (30.61%), BA (30.61%) and AComA (22.45%), while other fenestrations occurred infrequently. There were no significant differences in the age of patients in the individuals with vascular fenestration (p > 0.05). VA fenestration was slightly more common in men (16.67%) than in women (5.41%). However, these differences were not statistically significant (p = 0.216). Limitations: Our study has several limitations, including selection bias regarding examined population. Second, we assume that the total number of fenestrations detected in our study was underestimated due to the limitations of the CT method in comparison to other radiologic modalities. Conclusions: Cerebral arterial fenestrations are rare vascular malformations. The ACA is the most common localization of fenestrations, followed by BA and AComA. Fenestrations of cerebral arteries insignificantly increase the risk of cerebral aneurysm formation. Further prospective studies are necessary to make this association more precise.
Neuroticism and Conscientiousness Moderate the Effect of Oral Medication Beliefs on Adherence of People with Mental Illness during the Pandemic
Authors: Ferretti Fabio , Goracci Arianna , Laurenzi Pier Francesco , Centola Rossella , Crecchi Irene , De Luca Aldo , Monzillo Janette , Guidi Omar , Sinigaglia Giusy , Gualtieri Giacomo , Lorenzi Lore , Cuomo Alessandro , Bolognesi Simone , Travagli Valter , Coluccia Anna , Fagiolini Andrea , Pozza Andrea
Published: September 2022

Background. After the declaration of the pandemic status in several countries, the continuity of face-to-face visits in psychiatric facilities has been delayed or even interrupted to reduce viral spread. Little is known about the personality factors associated with medication beliefs and adherence amongst individuals with mental illness during the COVID-19 pandemic. This brief report describes a preliminary naturalistic longitudinal study that explored whether the Big Five personality traits prospectively moderate the effects of medication beliefs on changes in adherence during the pandemic for a group of outpatients with psychosis or bipolar disorder. Methods. Thirteen outpatients undergoing routine face-to-face follow-up assessments during the pandemic were included (41 observations overall) and completed the Revised Italian Version of the Ten-Item Personality Inventory, the Beliefs about Medicines Questionnaire, the Morisky Medication Adherence Scale—8-item and the Beck Depression Inventory—II. Results. Participants had stronger concerns about their psychiatric medications rather than beliefs about their necessity, and adherence to medications was generally low. Participants who had more necessity beliefs than concerns had better adherence to medications. People scoring higher in Conscientiousness and Neuroticism traits and more concerned about the medication side effects had poorer adherence. Conclusions. These preliminary data suggest the importance of a careful assessment of the adherence to medications amongst people with psychosis/bipolar disorder during the pandemic. Interventions aimed to improve adherence might focus on patients’ medication beliefs and their Conscientiousness and Neuroticism personality traits.
Study on Comprehensive Technology of Preventing Mud Cake of Large Diameter Slurry Shield in Composite Stratum
Authors: Mei Yuan , Zhou Dongbo , Gong Hang , Ke Xin , Xu Wangyang , Shi Wenyan
Published: September 2022

When large-diameter slurry shields are tunneling in a composite stratum with a high clay content, the cutter head may form a mud cake. After the mud cake is formed, it will cover the cutter and reduce the opening rate of the cutter head, thus reducing the penetration of the cutter and the driving speed. Based on a road upgrading project, this paper studies the prevention and treatment of a mud cake and forms a set of comprehensive treatment methods. For a large mud cake, through theoretical analysis, two calculation methods of the cutter head sealing coefficient are obtained, and the cutter head sealing coefficient relationship model is established. Comparing the calculated cutter head’s actual opening coefficient with the required cutter head opening coefficient can provide a judgment basis for the time of manual warehousing. For medium and small mud cakes, the numerical simulation is carried out based on the cutter head and the scouring system, the distribution characteristics of the flow field near the cutter head are analyzed, and the angle of the central scouring hole is optimized. For a small mud cake, hydrogen peroxide is selected as an additive through microscopic mechanism analysis. The effect of the hydrogen peroxide solution concentration on dissolving a mud cake is obtained through laboratory tests, and its effectiveness is verified through field tests. The research results can provide a reference for the mud cake prevention of slurry shields in similar strata.
The Impact of Race–Ethnicity and Diagnosis of Alzheimer’s Disease and Related Dementias on Mammography Use
Authors: Chen Aokun , Li Yongqiu , Woodard Jennifer N. , Islam Jessica Y. , Yang Shuang , George Thomas J. , Shenkman Elizabeth A. , Bian Jiang , Guo Yi
Published: September 2022

Breast cancer screening (BCS) with mammography is a crucial method for improving cancer survival. In this study, we examined the association of Alzheimer’s disease (AD) and AD-related dementias (ADRD) diagnosis and race–ethnicity with mammography use in BCS-eligible women. In the real-world data from the OneFlorida+ Clinical Research Network, we extracted a cohort of 21,715 BCS-eligible women with ADRD and a matching comparison cohort of 65,145 BCS-eligible women without ADRD. In multivariable regression analysis, BCS-eligible women with ADRD were more likely to undergo a mammography than the BCS-eligible women without ADRD (odds ratio [OR] = 1.19, 95% confidence interval [CI] = 1.13–1.26). Stratified by race–ethnicity, BCS-eligible Hispanic women with ADRD were more likely to undergo a mammography (OR = 1.56, 95% CI = 1.39–1.75), whereas BCS-eligible non-Hispanic black (OR = 0.72, 95% CI = 0.62–0.83) and non-Hispanic other (OR = 0.65, 95% CI = 0.45–0.93) women with ADRD were less likely to undergo a mammography. This study was the first to report the impact of ADRD diagnosis and race–ethnicity on mammography use in BCS-eligible women using real-world data. Our results suggest ADRD patients might be undergoing BCS without detailed guidelines to maximize benefits and avoid harms.
Arterial Spin Labeling Perfusion in Pediatric Brain Tumors: A Review of Techniques, Quality Control, and Quantification
Authors: Troudi Abir , Tensaouti Fatima , Baudou Eloise , Péran Patrice , Laprie Anne
Published: September 2022

Arterial spin labeling (ASL) is a magnetic resonance imaging (MRI) technique for measuring cerebral blood flow (CBF). This noninvasive technique has added a new dimension to the study of several pediatric tumors before, during, and after treatment, be it surgery, radiotherapy, or chemotherapy. However, ASL has three drawbacks, namely, a low signal-to-noise-ratio, a minimum acquisition time of 3 min, and limited spatial summarize current resolution. This technique requires quality control before ASL-CBF maps can be extracted and before any clinical investigations can be conducted. In this review, we describe ASL perfusion principles and techniques, summarize the most recent advances in CBF quantification, report technical advances in ASL (resting-state fMRI ASL, BOLD fMRI coupled with ASL), set out guidelines for ASL quality control, and describe studies related to ASL-CBF perfusion and qualitative and semi-quantitative ASL weighted-map quantification, in healthy children and those with pediatric brain tumors.
Transcriptome-Wide Gene Expression Profiles from FFPE Materials Based on a Nuclease Protection Assay Reveals Significantly Different Patterns between Synovial Sarcomas and Morphologic Mimickers
Authors: Borchert Sabrina , Herold Thomas , Kalbourtzis Stavros , Hamacher Rainer , Krause Yvonne , Berger Sophia , Guder Wiebke K. , Streitbuerger Arne , Hardes Jendrik , Goetz Moritz , Bauer Sebastian , Schildhaus Hans-Ulrich
Published: September 2022

Background: Transcriptome profiling provides large data on tumor biology, which is particularly valuable in translational research and is becoming more and more important for clinical decision-making as well. RNA sequencing is considered to be the gold standard for this. However, FFPE material, as the most available material in routine pathology, has been an undefeatable obstacle for RNAseq. Extraction-free nuclease protection assays have the potential to be a reliable alternative method for large-scale expression profiling. The aim of this study was to validate and test the basic feasibility, technical applicability robustness, and reliability of the HTG transcriptome profiling (HTP) assay on clinical tumor samples. Methods: FFPE samples from 44 synovial sarcomas (SyS) and 20 spindle cell sarcomas (SpcS) were used. The HTP assay was performed on 10 µm thin FFPE slides. After nuclease protection in the HTG Edge Seq System, libraries were generated for sequencing on an Illumina NextSeq 500 platform. Fastq data were parsed and then analyzed by using the HTG analysis platform EdgeSeq REVEAL. Immunohistochemistry was performed to validate the expression of TLE1. Results: The technical application of the HTP Panel revealed robust and reliable results with 62 samples, and only 2 samples failed due to an incomplete digestion of gDNA. The analysis, performed at the analysis platform REVEAL, showed 5964 genes being significantly differentially expressed between SpcS and SyS. In particular, overexpression of the known marker TLE1 in synovial sarcoma could be recovered, which underlines the reliability of this system. Discussion: Transcriptome profiling gets more and more important for tumor research and diagnostics. Among other established technologies, the HTP Panel has shown to be a feasible method to get robust and reliable results. Thereby, this method needs very few sample-input by getting a success-rate of 96.88%, which indicates the upper average range, compared to other technologies working with FFPE tissue. Conclusion: The nuclease protection assay-based HTP Panel is a feasible method for adequate transcriptome profiling with low sample input and therefore is suitable for further research of biomarkers.
Transfer Learning for Adenocarcinoma Classifications in the Transurethral Resection of Prostate Whole-Slide Images
Authors: Tsuneki Masayuki , Abe Makoto , Kanavati Fahdi
Published: September 2022

The transurethral resection of the prostate (TUR-P) is an option for benign prostatic diseases, especially nodular hyperplasia patients who have moderate to severe urinary problems that have not responded to medication. Importantly, incidental prostate cancer is diagnosed at the time of TUR-P for benign prostatic disease. TUR-P specimens contain a large number of fragmented prostate tissues; this makes them time consuming to examine for pathologists as they have to check each fragment one by one. In this study, we trained deep learning models to classify TUR-P WSIs into prostate adenocarcinoma and benign (non-neoplastic) lesions using transfer and weakly supervised learning. We evaluated the models on TUR-P, needle biopsy, and The Cancer Genome Atlas (TCGA) public dataset test sets, achieving an ROC-AUC up to 0.984 in TUR-P test sets for adenocarcinoma. The results demonstrate the promising potential of deployment in a practical TUR-P histopathological diagnostic workflow system to improve the efficiency of pathologists.
Magnetic Resonance Imaging-Based Predictive Models for Clinically Significant Prostate Cancer: A Systematic Review
Authors: Triquell Marina , Campistol Miriam , Celma Ana , Regis Lucas , Cuadras Mercè , Planas Jacques , Trilla Enrique , Morote Juan
Published: September 2022

MRI can identify suspicious lesions, providing the semi-quantitative risk of csPCa through the Prostate Imaging-Report and Data System (PI-RADS). Predictive models of clinical variables that individualise the risk of csPCa have been developed by adding PI-RADS score (MRI-PMs). Our objective is to analyse the current developed MRI-PMs and define their clinical usefulness. A systematic review was performed after a literature search performed by two independent investigators in PubMed, Cochrane, and Web of Science databases, with the Medical Subjects Headings (MESH): predictive model, nomogram, risk model, magnetic resonance imaging, PI-RADS, prostate cancer, and prostate biopsy. This review was made following the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) criteria and studied eligibility based on the Participants, Intervention, Comparator, and Outcomes (PICO) strategy. Among 723 initial identified registers, 18 studies were finally selected. Warp analysis of selected studies was performed with the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. Clinical predictors in addition to the PI-RADS score in developed MRI-PMs were age, PCa family history, digital rectal examination, biopsy status (initial vs. repeat), ethnicity, serum PSA, prostate volume measured by MRI, or calculated PSA density. All MRI-PMs improved the prediction of csPCa made by clinical predictors or imaging alone and achieved most areas under the curve between 0.78 and 0.92. Among 18 developed MRI-PMs, 7 had any external validation, and two RCs were available. The updated PI-RADS version 2 was exclusively used in 11 MRI-PMs. The performance of MRI-PMs according to PI-RADS was only analysed in a single study. We conclude that MRI-PMs improve the selection of candidates for prostate biopsy beyond the PI-RADS category. However, few developed MRI-PMs meet the appropriate requirements in routine clinical practice.
CuS-Based Nanostructures as Catalysts for Organic Pollutants Photodegradation
Authors: Isac Luminita , Cazan Cristina , Andronic Luminita , Enesca Alexandru
Published: September 2022

The direct or indirect discharge of toxic and non-biodegradable organic pollutants into water represents a huge threat that affects human health and the environment. Therefore, the treatment of wastewater, using sustainable technologies, is absolutely necessary for reusability. Photocatalysis is considered one of the most innovative advanced techniques used for pollutant removal from wastewater, due to its high efficiency, ease of process, low-cost, and the environmentally friendly secondary compounds that occur. The key of photocatalysis technology is the careful selection of catalysts, usually semiconductor materials with high absorption capacity for solar light, and conductivity for photogenerated charge carriers. Among copper sulfides, CuS (covellite), a semiconductor with different morphologies and bandgap values, is recognized as an important photocatalyst used for the removal of organic pollutants (dyes, pesticides, pharmaceutics etc.) from wastewater. This review deals with recent developments in organic pollutant photodegradation, using as catalysts various CuS nanostructures, consisting of CuS NPs, CuS QDs, and heterojunctions (CuS/ carbon-based materials, CuS/organic semiconductor, CuS/metal oxide). The effects of different synthesis parameters (Cu:S molar ratios, surfactant concentration etc.) and properties (particle size, morphology, bandgap energy, and surface properties) on the photocatalytic performance of CuS-based catalysts for the degradation of various organic pollutants are extensively discussed.
Cyanogel-Induced Synthesis of RuPd Alloy Networks for High-Efficiency Formic Acid Oxidation
Authors: Liu Qicheng , Yan Wei , Zhang Jiachen , Ren Yi , Liu Jiaqi , Zeng Xin , Sun Dongmei , Tang Yawen
Published: September 2022

For direct formic acid fuel cells (DFAFC), palladium (Pd)-based alloy catalysts with competitive morphology and elemental composition are essential to boost the performance of the formic acid oxidation reaction (FAOR) in the anode zone. Herein, we design and synthesize RuPdx alloy nano-network structures (ANs) via the facile wet-chemical reduction of Pd-Ru cyanogel (Pdx [Ru(CN)6]y·aH2O) as an effective electrocatalyst for the FAOR. The formation of Pd-Ru cyanogel depends on the facile coordination of K2PdCl4 and K3 [Ru(CN)6]. The unique structure of cyanogel ensures the presentation of a three-dimensional mesoporous morphology and the homogeneity of the elemental components. The as-prepared RuPd3 ANs exhibit good electrocatalytic activity and stability for the FAOR. Notably, the RuPd3 ANs achieve a mass-specific activity of 2068.4 mA mg−1 in FAOR, which shows an improvement of approximately 16.9 times compared to Pd black. Such a competitive FAOR performance of RuPd3 ANs can be attributed to the advantages of structure and composition, which facilitate the exposure of more active sites, accelerate mass/electron transfer rates, and promote gas escape from the catalyst layer, as well as enhance chemical stability.
Presynaptic 5-HT2A-mGlu2/3 Receptor–Receptor Crosstalk in the Prefrontal Cortex: Metamodulation of Glutamate Exocytosis
Authors: Taddeucci Alice , Olivero Guendalina , Roggeri Alessandra , Milanese Claudio , Giorgio Francesco Paolo Di , Grilli Massimo , Marchi Mario , Garrone Beatrice , Pittaluga Anna
Published: September 2022

The glutamatergic nerve endings of a rat prefrontal cortex (PFc) possess presynaptic 5-HT2A heteroreceptors and mGlu2/3 autoreceptors, whose activation inhibits glutamate exocytosis, and is measured as 15 mM KCl-evoked [3H]D-aspartate ([3H]D-asp) release (which mimics glutamate exocytosis). The concomitant activation of the two receptors nulls their inhibitory activities, whereas blockade of the 5-HT2A heteroreceptors with MDL11,939 (1 μM) strengthens the inhibitory effect elicited by the mGlu2/3 receptor agonist LY329268 (1 μM). 5-HT2A receptor antagonists (MDL11,939; ketanserin; trazodone) amplify the impact of low (3 nM) LY379268. Clozapine (0.1–10 μM) mimics the 5-HT2A agonist (±) DOI and inhibits the KCl-evoked [3H]D-asp overflow in a MDL11,939-dependent fashion, but does not modify the (±) DOI-induced effect. mGlu2 and 5-HT2A proteins do not co-immunoprecipitate from synaptosomal lysates, nor does the incubation of PFc synaptosomes with MDL11,939 (1 μM) or clozapine (10 µM) modify the insertion of mGlu2 subunits in synaptosomal plasma membranes. In conclusion, 5-HT2A and mGlu2/3 receptors colocalize, but do not physically associate, in PFc glutamatergic terminals, where they functionally interact in an antagonist-like fashion to control glutamate exocytosis. The mGlu2/3-5-HT2A metamodulation could be relevant to therapy for central neuropsychiatric disorders, including schizophrenia, but also unveil cellular events accounting for their development, which also influence the responsiveness to drugs regimens.
TIMP-1 Dependent Modulation of Metabolic Profiles Impacts Chemoresistance in NSCLC
Authors: Xiao Wei , Ahluwalia Pankaj , Wang Lan , Howard John , Kolhe Ravindra , Rojiani Amyn M. , Rojiani Mumtaz V.
Published: September 2022

The development of chemoresistance remains a significant barrier to treating NSCLC. Alteration of cancer cell metabolism is an important mechanism for chemoresistance. This study explored the role of aberrant metabolism in TIMP-1-mediated chemoresistance. Bioinformatics analysis identified an association of high TIMP-1 with altered energy metabolism. We have defined the role of depolarized mitochondria through a reduction in lactate secretion, higher ROS levels in TIMP-1 KD cells and reduced GSH levels. TIMP-1 modulates the metabolic profile via acetylation of mitochondrial STAT3 and its interaction with CD44. Intriguingly, monomers of acetylated STAT3 were critical for altered metabolism, whereas STAT3 dimers abrogated this function. Further, the mitochondrial metabolic profile was also altered in a cisplatin-resistant clone of A549 cells. We also correlated the immunoexpression of CD44, STAT3 and TIMP-1 in patient samples. This study provided evidence that TIMP-1 alters the metabolic profile by modulating mitochondrial metabolism via the CD44-STAT3 axis through its effects on STAT3 acetylation. It also lent further support to the critical role of TIMP-1 in chemoresistance. Interrogation of the TCGA-LUAD dataset revealed perturbations in the critical modulator that can alter metabolic states in cancer cells. Higher expression of a five-gene signature, including TIMP-1, correlated with immunosuppressive cells and was found to be associated with overall survival. This study identified several metabolic mechanisms that could influence therapeutic options and prognosis in NSCLC patients.
Glucose 6-P Dehydrogenase—An Antioxidant Enzyme with Regulatory Functions in Skeletal Muscle during Exercise
Authors: García-Domínguez Esther , Carretero Aitor , Viña-Almunia Aurora , Domenech-Fernandez Julio , Olaso-Gonzalez Gloria , Viña Jose , Gomez-Cabrera Mari Carmen
Published: September 2022

Hypomorphic Glucose 6-P dehydrogenase (G6PD) alleles, which cause G6PD deficiency, affect around one in twenty people worldwide. The high incidence of G6PD deficiency may reflect an evolutionary adaptation to the widespread prevalence of malaria, as G6PD-deficient red blood cells (RBCs) are hostile to the malaria parasites that infect humans. Although medical interest in this enzyme deficiency has been mainly focused on RBCs, more recent evidence suggests that there are broader implications for G6PD deficiency in health, including in skeletal muscle diseases. G6PD catalyzes the rate-limiting step in the pentose phosphate pathway (PPP), which provides the precursors of nucleotide synthesis for DNA replication as well as reduced nicotinamide adenine dinucleotide phosphate (NADPH). NADPH is involved in the detoxification of cellular reactive oxygen species (ROS) and de novo lipid synthesis. An association between increased PPP activity and the stimulation of cell growth has been reported in different tissues including the skeletal muscle, liver, and kidney. PPP activity is increased in skeletal muscle during embryogenesis, denervation, ischemia, mechanical overload, the injection of myonecrotic agents, and physical exercise. In fact, the highest relative increase in the activity of skeletal muscle enzymes after one bout of exhaustive exercise is that of G6PD, suggesting that the activation of the PPP occurs in skeletal muscle to provide substrates for muscle repair. The age-associated loss in muscle mass and strength leads to a decrease in G6PD activity and protein content in skeletal muscle. G6PD overexpression in Drosophila Melanogaster and mice protects against metabolic stress, oxidative damage, and age-associated functional decline, and results in an extended median lifespan. This review discusses whether the well-known positive effects of exercise training in skeletal muscle are mediated through an increase in G6PD.
Provisional Matrix Formation at Implant Surfaces—The Bridging Role of Calcium Ions
Authors: Anitua Eduardo , Tejero Ricardo
Published: September 2022

The success of dental implants lies in their strong and lasting integration into the patient’s receiving bone. The first biological interactions at the implant surface determine the subsequent evolution of the integration process. In this study we set our objective to analyze the mechanistic interaction of the early regenerative matrix at implant surfaces modified with calcium ions (Ca) as compared to standard implant surfaces (NoCa). We put the surfaces in a Quartz Crystal Microbalance with Dissipation (QCM-D) to monitor the frequency shift (f) and the viscoelastic properties of the adsorbed biofilms and used Scanning Electron Microscopy (SEM) to visualize the resulting interfaces. Upon the addition of human blood plasma, Ca surfaces formed an adsorbed three-dimensional film attached to the surface (∆f = −40 Hz), while with NoCa, the biofilm formed but was not attached to the surface (∆f = 0 Hz). After 20 min in blood, two representative commercial implants with Ca and NoCa surfaces showed also distinct interfaces: Ca implants formed a visible clot attached to the implant which was composed mainly of platelets (Surface Coverage: 40 ± 20%) and some red blood cells (SC: 9 ± 3%) entrapped within a fibrin network (SC: 93 ± 5%). The NoCa implants were largely populated by red blood cells (SC: 67 ± 12%) with scarce fibrin remnants (SC: 3 ± 2%), and the implants showed no clot on their surfaces macroscopically. The pre-clinical and clinical results discussed in this work encourage the modification of titanium implant surfaces with calcium ions to improve the bone regenerative process. Taken together, these results add more information about the roles of Ca ions in bridging the formation of the provisional matrix at implant surfaces and their effects on implant osseointegration.
Role of Volume Replacement during Neonatal Resuscitation in the Delivery Room
Authors: Sankaran Deepika , Lane Emily C. A. , Valdez Rebecca , Lesneski Amy L. , Lakshminrusimha Satyan
Published: September 2022

Volume expanders are indicated in the delivery room when an asphyxiated neonate is not responding to the steps of neonatal resuscitation and has signs of shock or a history of acute blood loss. Fetal blood loss (e.g., feto-maternal hemorrhage) may contribute to perinatal asphyxia. Cord compression or a tight nuchal cord can selectively occlude a thin-walled umbilical vein, resulting in feto-placental transfusion and neonatal hypovolemia. For severe bradycardia or cardiac arrest secondary to fetal blood loss, Neonatal Resuscitation Program (NRP) recommends intravenous volume expanders (crystalloids such as normal saline or packed red blood cells) infused over 5 to 10 min. Failure to recognize hypovolemia and subsequent delay in volume replacement may result in unsuccessful resuscitation due to lack of adequate cardiac preload. However, excess volume load in the presence of myocardial dysfunction from hypoxic–ischemic injury may precipitate pulmonary edema and intraventricular hemorrhage (especially in preterm infants). Emergent circumstances and ethical concerns preclude the performance of prospective clinical studies evaluating volume replacement during neonatal resuscitation. Translational studies, observational data from registries and clinical trials are needed to investigate and understand the role of volume replacement in the delivery room in term and preterm neonates. This article is a narrative review of the causes and consequences of acute fetal blood loss and available evidence on volume replacement during neonatal resuscitation of asphyxiated neonates.
A Pilot COVID-19 Surveillance Program at the Zendrini Center in Milan (Italy) for Unaccompanied Foreign Minors
Authors: Tambuzzi Stefano , Cummaudo Marco , Maggioni Lidia , Tritella Stefania , Lucchesi Barbara , Montedoro Paola , Agostinelli Immacolata , Trezzi Sofia , Costantino Antonella Maria , Mazzoni Rossana , Marognoli Michela , Poppa Pasquale , De Angelis Danilo , Cattaneo Cristina
Published: September 2022

During the COVID-19 pandemic, not only crowded refugee camps and immigration detention centers, but also receptions were places in which outbreaks occurred. To date there has been no report of the application of a COVID-19 surveillance system in reception centers for unaccompanied foreign minors only, who most of all deserve the utmost attention. Aware of this critical issue, we implemented a pilot COVID-19 surveillance program at the Zendrini center in Milan. It was started in September 2021 and was carried out for 4 months. Nasopharyngeal antigenic swabs were adopted. One day a week, two forensic physicians performed the first antigenic swab to minors who had just entered the center, or a monitoring swab after 15 days to those who were still hosted at the center. Operators were also swabbed for surveillance. A total of 80 subjects were enrolled and divided into 68 (72.5%) unaccompanied foreign minors and 22 (27.5%) operators. A total of 178 antigenic nasopharyngeal swabs were performed and tested negative. Regarding the monitoring activities, it was found that the minimum number of swabs per subject was 1 and the maximum number was 7, with an average value of 2.2 per individual. Having been able to confirm the absence of SARS-CoV-2 within the community represented a way to protect individual and collective health that could not have been pursued otherwise. Only inclusive approaches can allow communities and societies to respond more effectively to this crisis, and reduce the risk of future ones, intended as both upcoming COVID-19 waves and new infectious diseases.
Health-Related Quality of Life after Fractures of the Distal Forearm in Children and Adolescents—Results from a Center in Switzerland in 432 Patients
Authors: Liebs Thoralf Randolph , Lorance Alex , Berger Steffen Michael , Kaiser Nadine , Ziebarth Kai
Published: September 2022

(1) Background: We aimed to evaluate the health-related quality of life (HRQoL) in children with fractures of the distal forearm and to assess if HRQoL was associated with fracture classification; (2) Methods: We followed up on 432 patients (185 girls, 247 boys) who sustained a fracture of the distal radius or forearm from 1/2007 to 6/2007, 1/2014 to 6/2014, and 11/2016 to 10/2017. Patients filled in the Quick-DASH (primary outcome) and the Peds-QL; (3) Results: The radius was fractured in 429 and the ulna in 175 cases. The most frequent injury of the radius was a buckle fracture (51%, mean age 8.5 years), followed by a complete metaphyseal fracture (22%, 9.5 years), Salter-Harris-2 fracture (14%, 11.4 years), greenstick fracture (10%, 9.3 years), Salter-Harris-1 fracture (1%, 12.6 years), and other rare injuries. The most common treatment was closed reduction and an above-elbow cast in 138 cases (32%), followed by a cast without reduction (30%), splint (28%), and K-wire fixation and cast (9%). Definite treatment was performed initially in 95.8%, a new cast or cast wedging was performed in 1.6%, and revision surgery was performed in 2.5%. There were no open reductions and no plate fixations. After a mean follow-up of 4.2 years, patients with buckle fractures had a mean Quick-DASH of 3.3 (scale of 0–100) (complete fracture: 1.5; greenstick: 1.5; SH-1: 0.9; SH-2: 4.1; others: 0.9). The mean function score of the PedsQL ranged from 93.0 for SH-2 fractures to 97.9 for complete fractures; (4) Conclusions: In this cohort of 432 children with fractures of the distal forearm, there was equally good mean mid- and long-term HRQoL when assessed by the Quick-DASH and the PedsQL. There was a trend for children with complete metaphyseal fractures reporting better HRQoL than patients with buckle fractures or patients with Salter-Harris II fractures, however, these differences were not statistically significant nor clinically relevant.