bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into Multi-Model Federated Learning: An Advanced Approach for Air Quality Index Forecasting
Authors: Le Duy-Dong , Tran Anh-Khoa , Dao Minh-Son , Nguyen-Ly Kieu-Chinh , Le Hoang-Son , Nguyen-Thi Xuan-Dao , Pham Thanh-Qui , Nguyen Van-Luong , Nguyen-Thi Bach-Yen
Published: November 2022

The air quality index (AQI) forecast in big cities is an exciting study area in smart cities and healthcare on the Internet of Things. In recent years, a large number of empirical, academic, and review papers using machine learning (ML) for air quality analysis have been published. However, most of those studies focused on traditional centralized processing on a single machine, and there had been few surveys of federated learning (FL) in this field. This overview aims to fill this gap and provide newcomers with a broader perspective to inform future research on this topic, especially for the multi-model approach. In this survey, we went over the works that previous scholars have conducted in AQI forecast both in traditional ML approaches and FL mechanisms. Our objective is to comprehend previous research on AQI prediction including methods, models, data sources, achievements, challenges, and solutions applied in the past. We also convey a new path of using multi-model FL, which has piqued the computer science community’s interest recently.
Drivers of the Sharing Economy That Affect Consumers’ Usage Behavior: Moderation of Perceived Risk
Authors: Anaya Óscar , De La Vega Iván
Published: November 2022

In the access to peer-to-peer sharing of goods and services through a technology platform, which is known as the sharing economy, there is no consensus on the factors that motivate consumers. This study aimed to investigate the moderating effect of perceived risk on consumers’ participation in the sharing economy in a developing country. Following a quantitative approach, a survey was conducted among 400 consumers in the Metropolitan Zone of Puebla City, Mexico. Partial least squares structural equation modeling (PLS-SEM) was used to analyze the data. Economic benefits, enjoyment, and trust drove the usage behavior of consumers in the sharing economy. In addition, perceived risk significantly moderated the relationships that usage behavior has with the economic benefits and the feeling of the community. As predicted by social exchange theory, the consumers made choices based on a subjective cost–benefit analysis, showing flexibility in the type and amount of rewards. This study contributes to knowledge about customer behavior in the context of the sharing economy.
AI-Based Exhaust Gas Temperature Prediction for Trustworthy Safety-Critical Applications
Authors: Apostolidis Asteris , Bouriquet Nicolas , Stamoulis Konstantinos P.
Published: November 2022

Data-driven condition-based maintenance (CBM) and predictive maintenance (PdM) strategies have emerged over recent years and aim at minimizing the aviation maintenance costs and environmental impact by the diagnosis and prognosis of aircraft systems. As the use of data and relevant algorithms is essential to AI-based gas turbine diagnostics, there are different technical, operational, and regulatory challenges that need to be tackled in order for the aeronautical industry to be able to exploit their full potential. In this work, the machine learning (ML) method of the generalised additive model (GAM) is used in order to predict the evolution of an aero engine’s exhaust gas temperature (EGT). Three different continuous synthetic data sets developed by NASA are employed, known as New Commercial Modular Aero-Propulsion System Simulation (N-CMAPSS), with increasing complexity in engine deterioration. The results show that the GAM can be predict the evolution of the EGT with high accuracy when using several input features that resemble the types of physical sensors installed in aero gas turbines currently in operation. As the GAM offers good interpretability, this case study is used to discuss the different data attributes a data set needs to have in order to build trust and move towards certifiable models in the future.
Spatial Distribution of Salmonella in Soil near Municipal Waste Landfill Site
Authors: Frączek Krzysztof , Ropek Dariusz Roman , Kozdrój Jacek
Published: November 2022

Due to the heterogeneous origin of municipal waste, different substrates support the growth of many microorganisms, including those hazardous to humans. In consequence, landfills collecting these refuses are regarded as serious sources of infectious material contaminating the environment. In this study, we aimed to assess how waste may be related to the presence of Salmonella spp. in soil within a landfill and its surroundings. The numbers of these pathogens were estimated in soil samples collected at 17 different stands established in the municipal waste landfill of Barycz (near Kraków, Poland) and the surrounding area. The analysis showed that in all soil samples, Salmonella spp. did not exceed 270 cfu g−1 in dry soil (i.e., the active landfill sector). Salmonella spp. was found in 57% of the tested soil samples in spring, 88% in summer, 45% in autumn, and was not detected in winter. A spatial distribution visualized by graphical maps allowed determination of the influence of the active sector on the surrounding areas. The graphical maps showed the impact of seasons on the spread of Salmonella spp. in the soil near the landfill. Detection and estimation of Salmonella spp. distribution in soil within the landfill area distinctly confirms the hazardous impact of collected wastes on hygienic characteristics of the soil.
Study on the Technologies of Loss Reduction in Wheat Mechanization Harvesting: A Review
Authors: Chen Xu , He Xun , Wang Wanzhang , Qu Zhe , Liu Yuan
Published: November 2022

Wheat harvesting is one of the most important links in the whole wheat production process. In China, the wheat planting areas are wide, and the patterns are diversified. In addition, the problem of harvest losses caused by the numerous brands and low performance of domestic combine harvesters has always existed. Any losses during harvesting will result in less income for the farmers. Therefore, according to the actual situation of mechanized wheat harvesting and the losses occurring within different parts of the harvester, it is of great significance to select the appropriate loss reduction methods to effectively reduce wheat harvest losses. In accordance with the problems of loss during mechanized harvesting, this research first points out the main losses in the operation of a wheat combine harvester, then introduces sensor monitoring technology for grain harvesting loss and intelligent control technology for the combine harvester and analyzes their application to loss reduction in mechanized wheat harvesting. Finally, we put forward conclusions and suggestions on this loss reduction technology for wheat mechanization harvesting in order to provide a reference for reducing the losses and promoting the sustainable development of modern agriculture.
Development and Validation of Pesticide Residues Determination Method in Fruits and Vegetables through Liquid and Gas Chromatography Tandem Mass Spectrometry (LC-MS/MS and GC-MS/MS) Employing Modified QuEChERS Method and a Centrifugal Vacuum Concentrator
Authors: Romniou Styliani E. , Nana Konstantina , Dasenaki Marilena , Komaitis Efstratios , Proestos Charalampos
Published: November 2022

Pesticides are used for controlling organisms, weeds and animals, causing damage to plants. Although the use of pesticides is a prerequisite for producing safe food, their accumulation makes their rapid determination necessary to avoid negative impacts on human health. The aim of this study was to develop reliable and robust analytical methods for the determination of pesticide residues in fruits and vegetables, validated according to SANTE/12682/2019 guidance. Five different categories of fruits and vegetables were selected (apple, orange, onion, lettuce, tomato). The sample preparation was based on QuEChERS methodology, slightly modified in the clean-up step, and appropriate d-SPE reagents were selected for each commodity. A Multi-Tube Vortexer was used for better agitation. In the final step, the extract was split in two: one part was acidified and injected in LC-MS/MS and the other part was evaporated in a centrifugal vacuum concentrator, and reconstituted and injected in GC-MS/MS. With the centrifugal vacuum concentrator used instead of nitrogen stream, more pesticides were determined, while sensitivity and repeatability increased. Validation results satisfied the SANTE/12682/2019 guidelines for approximately 220 analytes for each commodity. LOQ was set at 0.010 mg/kg for all analytes. Successful external quality assessment (proficiency testing) proved that the methods are fit for purpose.
R&D Performance Evaluation in the Chinese Food Manufacturing Industry Based on Dynamic DEA in the COVID-19 Era
Authors: Mao Shiping , Kremantzis Marios Dominikos , Kyrgiakos Leonidas Sotirios , Vlontzos George
Published: November 2022

Nowadays, China’s food consumption structure is shifting from being survival-oriented to health-oriented. However, the food industry is still facing a research and development (R&D) dilemma. Scientific evaluation of an enterprise’s R&D performance can help to reduce the investment risk of R&D and promote economic benefits. This study implements the dynamic data envelopment analysis (DDEA) technique to measure and evaluate the level of R&D performance in the Chinese food manufacturing industry. Twenty-eight listed companies were selected for the study, considering the time period from 2019 to 2021. After constructing a system of inputs, outputs and carry-over indicators, overall and period efficiency scores were obtained. The results reveal that the overall level of R&D in the industry is relatively low (0.332). Average efficiency scores across years were estimated as 0.447, 0.460, 0.430 for 2019, 2020, and 2021, respectively. Lastly, this study considers the actual business situation of the industry and makes suggestions for improvement from the perspective of enterprises and the government; these anticipate aiding the food manufacturing industry to improve the performance management of R&D activities.
Brachiaria humidicola Cultivation Enhances Soil Nitrous Oxide Emissions from Tropical Grassland by Promoting the Denitrification Potential: A 15N Tracing Study
Authors: Xie Lu , Liu Deyan , Müller Christoph , Jansen-Willems Anne , Chen Zengming , Niu Yuhui , Zaman Mohammad , Meng Lei , Ding Weixin
Published: November 2022

Biological nitrification inhibition (BNI) in the tropical grass Brachiaria humidicola could reduce net nitrification rates and nitrous oxide (N2O) emissions in soil. To determine the effect on gross nitrogen (N) transformation processes and N2O emissions, an incubation experiment was carried out using 15N tracing of soil samples collected following 2 years of cultivation with high-BNI Brachiaria and native non-BNI grass Eremochloa ophiuroide. Brachiaria enhanced the soil ammonium (NH4+) supply by increasing gross mineralization of recalcitrant organic N and the net release of soil-adsorbed NH4+, while reducing the NH4+ immobilization rate. Compared with Eremochloa, Brachiaria decreased soil gross nitrification by 37.5% and N2O production via autotrophic nitrification by 14.7%. In contrast, Brachiaria cultivation significantly increased soil N2O emissions from 90.42 μg N2O-N kg−1 under Eremochloa cultivation to 144.31 μg N2O-N kg−1 during the 16-day incubation (p < 0.05). This was primarily due to a 59.6% increase in N2O production during denitrification via enhanced soil organic C, notably labile organic C, which exceeded the mitigated N2O production rate during nitrification. The contribution of denitrification to emitted N2O also increased from 9.7% under Eremochloa cultivation to 47.1% in the Brachiaria soil. These findings confirmed that Brachiaria reduces soil gross nitrification and N2O production via autotrophic nitrification while efficiently stimulating denitrification, thereby increasing soil N2O emissions.
Effects of Copper Sulfate and Encapsulated Copper Addition on In Vitro Rumen Fermentation and Methane Production
Authors: Wilk Martyna , Pecka-Kiełb Ewa , Pastuszak Jerzy , Asghar Muhammad Umair , Mól Laura
Published: November 2022

Copper is a microelement crucial for the proper functioning of animals’ metabolic processes. The function of copper in rumen fermentation processes and methanogenesis is not well analyzed. The aim of the study was to evaluate the different types of copper supplement, their rumen decomposition and effect on in vitro ruminal fermentation as well as methanogenesis. Two different copper additives were used in the experiment: CS—copper sulfate (CuSO4 × 5 H2O)—and EC—encapsulated copper (tribasic copper chloride and copper sulfate enclosed within a polysaccharide polymer coating). A total mixed ration without copper additive was used as a control (C). In vitro rumen fermentation was conducted, and fermentation profile, gas production and methanogenesis were evaluated. After 24 h of fermentation, the amount of copper in the rumen fluid was significantly higher in the CS group. EC was protected against rumen degradation to a greater extent. The type of used copper supplement affects rumen fermentation. However, the effect on methanogenesis is ambiguous. CS supplement increases rumen gas production but does not affect methanogenesis. The obtained results suggest that the EC supplement may reduce the risk of low-fat milk and may improve the economic indicators of milk production. An in vivo experiment is necessary to compare the obtained in vitro results with animal productivity.
Allelopathic Activity of Annona reticulata L. Leaf Extracts and Identification of Three Allelopathic Compounds for the Development of Natural Herbicides
Authors: Khatun Mst. Rokeya , Tojo Shunya , Teruya Toshiaki , Kato-Noguchi Hisashi
Published: November 2022

Using plant-based allelopathic compounds might be a potent substitute to help mitigate the effects of synthetic herbicides. Annona reticulata L. is often planted for its fruit in residential gardens. This plant is well-documented for its diverse ethnomedicinal uses. However, there is no information in the literature on the allelopathic potential of A. reticulata leaves. Therefore, the allelopathic potential and relevant allelopathic compounds of A. reticulata leaves were investigated in this study. The bioassays were carried out using a completely randomized experimental layout (CRD), and the resulting data were analyzed using one-way ANOVA at p ≤ 0.05. Aqueous methanol extracts of A. reticulata leaves significantly inhibited the growth of three dicots and three monocots (Lepidium sativum L., Medicago sativa L., Lactuca sativa L., Echinochloa crus-galli (L.) P. Beauv., Lolium multiflorum Lam., and Phleum pratense L., respectively). The level of growth inhibition was proportional to the A. reticulata extract concentration. Three compounds were purified through different chromatographic steps, and their structures were determined using spectroscopy and identified as loliolide, 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one, and 3,4-dihydroxyphenylethanol. The 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one had the greatest effect on suppressing cress root growth, while loliolide had the greatest effect on suppressing timothy shoot growth. The values for 50% seedling growth suppression showed that the compound with the maximum inhibitory activity was loliolide, followed by 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one and 3,4-dihydroxyphenylethanol. Therefore, this result suggests that the three compounds might be responsible for the allelopathic effects of A. reticulata leaf extracts, and these compounds have the potential to be used to develop effective bioherbicides.
Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen–Thawed Rooster Sperm?
Authors: Sun Lingwei , He Mengqian , Xu Jiehuan , Wu Caifeng , Zhang Shushan , Zhang Defu , Dai Jianjun , Gao Jun
Published: November 2022

In this study, we aimed to determine the benefit of mitoquinone (MitoQ) in rooster semen extenders on sperm quality, motility parameters, antioxidant capacities, and apoptotic changes in post-thawed rooster semen. A total of 85 ejaculates from 18 roosters were collected and then divided into five equal aliquots and cryopreserved in extenders with 1.0% soy lecithin nanoparticles that contained various concentrations of MitoQ (0 nM (M0), 50 nM (M50), 100 nM (M100), 150 nM (M150), and 200 nM (M200)). By using a computer-assisted semen analyzer, sperm motility parameters were assessed after freeze thawing. The M150 group had significantly higher percentages of total motility, progressive motility, viability, acrosome membrane integrity, and mitochondrial activity than the other groups (p < 0.05). Compared to other groups, M100 and M150 groups produced a higher percentage of plasma membrane integrity and ATP contents (p < 0.05). Additionally, the lowest levels of ROS and MDA in spermatozoa were observed in M150 group (p < 0.05), whereas the highest levels of ROS and MDA were observed in sperm in the controls or the M200 group (p < 0.05). Significantly higher values of SOD, GPx, and Cas-3 were found in the M150 group compared to other groups (p < 0.05). Overall, these results demonstrate that MitoQ at 150 nM not only ameliorates post-thawed sperm quality and motility parameters by restoring ATP levels and preventing membrane damage, but also improves redox balance and antiapoptotic activities.
Genetic and Phenotypic Parameters for Pelt Quality and Body Length and Weight Traits in American Mink
Authors: Valipour Shafagh , Karimi Karim , Barrett David , Do Duy Ngoc , Hu Guoyu , Sargolzaei Mehdi , Wang Zhiquan , Miar Younes
Published: November 2022

Understanding the genetics of fur characteristics and skin size is important for developing effective breeding programs in the mink industry. Therefore, the objectives of this study were to estimate the genetic and phenotypic parameters for pelt quality traits including live grading overall quality (LQU), live grading nap size (LNAP), dried pelt size (DPS), dried pelt nap size (DNAP) and overall quality of dried pelt (DQU), and body length and weight traits, including November body weight (Nov_BW), November body length (Nov_BL), harvest weight (HW) and harvest length (HL) in American mink. Dried pelt quality traits on 1195 mink and pelt quality traits on live animals on 1680 were collected from mink raised at two farms, in Nova Scotia and Ontario. A series of univariate analyses were implemented in ASReml 4.1 software to identify the significance (p < 0.05) of random effects (maternal genetic effects, and common litter effects) and fixed effects (farm, sex, color type, year, and age) for each trait. Subsequently, bivariate models were used to estimate the genetic and phenotypic parameters using ASReml 4.1. Heritability (±SE) estimates were 0.41 ± 0.06 for DPS, 0.23 ± 0.10 for DNAP, 0.12 ± 0.04 for DQU, 0.28 ± 0.06 for LQU, 0.44 ± 0.07 for LNAP, 0.29 ± 0.10 for Nov_BW, 0.28 ± 0.09 for Nov_BL, 0.41 ± 0.07 for HW and 0.31 ± 0.06 for HL. DPS had high positive genetic correlations (±SE) with Nov_BW (0.89 ± 0.10), Nov_BL (0.81 ± 0.07), HW (0.85 ± 0.05) and HL (0.85 ± 0.06). These results suggested that body weight and length measured on live animals in November of the first year were reliable indicators of dried pelt size. DQU had favorable genetic correlations with Nov_BL (0.55 ± 0.24) and HL (0.46 ± 0.20), and nonsignificant genetic correlations with DNAP (0.13 ± 0.25), Nov_BW (0.25 ± 0.25) and HW (0.06 ± 0.20), which made body length traits an appealing trait for selection for increased pelt size. High positive genetic correlation (±SE) was observed between LNAP and DNAP (0.82 ± 0.22), which revealed that nap size measurement on live animals is a reliable indicator trait for dried pelt nap size. However, nonsignificant (p > 0.05) low genetic correlation (±SE) was obtained between LQU and DQU (0.08 ± 0.45), showing that indirect selection based on live grading might not lead to the satisfactory improvement of dried pelt overall quality. The estimated genetic parameters for live grading, dried pelt quality, and body weight and body length traits may be incorporated into breeding programs to improve fur characteristics in Canadian mink populations.
Comparative Histology of the Cornea and Palisades of Vogt in the Different Wild Ruminants (Bovidae, Camelidae, Cervidae, Giraffidae, Tragulidae)
Authors: Klećkowska-Nawrot Joanna , Goździewska-Harłajczuk Karolina , Barszcz Karolina
Published: November 2022

In the study, we data concerning the histological and morphometrical examination of the cornea and palisades of Vogt in the different species of ruminants from the families Bovidae, Camelidae, Cervidae, Giraffidae and Tragulidae, coming from the Warsaw Zoological Garden, the Wroclaw Zoological Garden and the Division of Animal Anatomy. The following ruminant species were investigated: common wildebeest, Kirk’s dik-dik, Natal red duiker, scimitar oryx, sitatunga, Philippine spotted deer, Père David’s deer, moose, reindeer, reticulated giraffe, okapi, Balabac mouse-deer and alpaca. The cornea of ruminant species such as the common wildebeest, Kirk’s dik-dik, Natal red duiker, scimitar oryx, reindeer and Balabac mouse-deer consisted of four layers (not found in the Bowman’s layer): the anterior corneal epithelium, the proper substance of the cornea, the posterior limiting membrane (Descemet’s membrane) and the posterior corneal epithelium (endothelium). The anterior corneal epithelium was composed of a multilayer keratinizing squamous epithelium, which was characterized in the studied ruminants with a variable number of cell layers but also with a different thickness both in the central epithelium part and in the peripheral part. Moreover, the proper substance of cornea was thinnest in Balabac mouse-deer, Kirk’s dik-dik, Natal red duiker, scimitar oryx, Philippine spotted deer, alpaca, reindeer and sitatunga and was thickest in the reticulated giraffe. The thickest Descemet’s membrane was observed in the Père David’s deer. The corneal limbus is characterized by a large number of pigment cell clusters in Kirk’s dik-dik, scimitar oryx, moose, Balabac mouse-deer and alpaca. In the common wildebeest, Père David’s deer, moose, reticulated giraffe, okapi and alpaca, the palisades of Vogt were marked in the form of a crypt-like structure. The corneal limbus epithelium in the examined ruminants was characterized by a variable number of cell layers but also a variable number of melanocytes located in different layers of this epithelium. The detailed knowledge of the corneal structure of domestic and wild animals can contribute to the even better development of methods for treating eye diseases in veterinary medicine.
Prevalence of Extended-Spectrum β-Lactamase-Resistant Genes in Escherichia coli Isolates from Central China during 2016–2019
Authors: Wang Zui , Lu Qin , Mao Xiaohui , Li Li , Dou Junfeng , He Qigai , Shao Huabin , Luo Qingping
Published: November 2022

The emergence and dissemination of Escherichia coli (E. coli) strains that produce extended-spectrum beta-lactamases (ESBLs) represents a major public health threat. The present study was designed to evaluate the prevalence and characteristics of ESBL-producing Escherichia coli isolates from chickens in central China during 2016–2019. A total of 407 E. coli strains isolated from 581 chicken swabs were identified conventionally and analyzed for various cephalosporin susceptibility by disk-diffusion assay. ESBL-producing strains were screened using the double=disk synergy test and ESBL-encoding genes were carried out by PCR/sequencing. A total of 402 E. coli isolates exhibited strong resistance to first- to fourth-generation cephalosporins and monobactam antibiotics, especially cefazolin (60.69%), cefuroxime (54.05%), cefepime (35.14%), ceftriaxone (54.30%), and aztreonam (40.29%). Piperacillin/tazobactam (1.72%) was the most effective drug against the strains, but the resistance rates increased each year. Among the isolates, 262 were identified as ESBL producers and the isolation rates for the ESBL producers increased from 63.37% to 67.35% over the four years. CTX-M (97.33%) was the most prevalent type, followed by TEM (76.72%) and SHV (3.05%). The most common ESBL genotype combination was blaTEM + blaCTX-M (74.46%), in which the frequency of carriers increased steadily, followed by blaCTX-M + blaSHV (3.05%). In addition, the most predominant specific CTX-M subtypes were CTX-M-55 (48.47%) and CTX-M-1 (17.94%), followed by CTX-M-14 (11.01%), CTX-M-15 (8.02%), CTX-M-9 (6.11%), CTX-M-65 (4.58%), and CTX-M-3 (1.15%). Moreover, a novel multiplex qPCR assay was developed to detect blaCTX-M, blaTEM, and blaSHV, with limits of detection of 2.06 × 101 copies/μL, 1.10 × 101 copies/μL, and 1.86 × 101 copies/μL, respectively, and no cross-reactivity with other ESBL genes and avian pathogens. The assays exhibited 100% sensitivity and specificities of 85%, 100%, and 100% for blaCTX-M, blaTEM, and blaSHV, respectively. In conclusion, our findings indicated that ESBL-producing E.coli strains isolated from chickens in central China were highly resistant to cephalosporins and frequently harbored diversity in ESBL-encoding genes. These isolates can pose a significant public health risk. The novel multiplex qPCR method developed in this study may be a useful tool for molecular epidemiology and surveillance studies of ESBL genes.
Dietary Se-Enriched Cardamine enshiensis Supplementation Alleviates Transport-Stress-Induced Body Weight Loss, Anti-Oxidative Capacity and Meat Quality Impairments of Broilers
Authors: Xu Xiao , Zhu Yunfen , Wei Yu , Chen Xiaofei , Li Rong , Xie Junhui , Wang Guogui , Ming Jiajia , Yin Hongqing , Xiang Jiqian , Huang Feiruo , Yang Yongkang
Published: November 2022

The aim of this experiment was to explore the effects of a new selenium (Se) source from Se-enriched Cardamine enshiensis (SeCe) on body weight loss, anti-oxidative capacity and meat quality of broilers under transport stress. A total of 240 one-day-old ROSS 308 broilers were allotted into four treatments with six replicate cages and 10 birds per cage using a 2 × 2 factorial design. The four groups were as follows: (1) Na2SeO3-NTS group, dietary 0.3 mg/kg Se from Na2SeO3 without transport stress, (2) SeCe-NTS group, dietary 0.3 mg/kg Se from SeCe without transport stress, (3) Na2SeO3-TS group, dietary 0.3 mg/kg Se from Na2SeO3 with transport stress, and (4) SeCe-TS group, dietary 0.3 mg/kg Se from SeCe with transport stress. After a 42 d feeding period, the broilers were transported by a lorry or kept in the original cages for 3 h, respectively. The results showed that dietary SeCe supplementation alleviated transport-stress-induced body weight loss and hepatomegaly of the broilers compared with the broilers fed Na2SeO3 diets (p < 0.05). Furthermore, dietary SeCe supplementation increased the concentrations of plasma total protein and glucose, and decreased the activities of aspartate aminotransferase and alanine aminotransferase of the broilers under transport stress (p < 0.05). Dietary SeCe supplementation also enhanced the anti-oxidative capacity and meat quality in the breast and thigh muscles of the broilers under transport stress (p < 0.05). In summary, compared with Na2SeO3, dietary SeCe supplementation alleviates transport-stress-induced body weight loss, anti-oxidative capacity and meat quality impairments of broilers.
High Intake of Sodium Chloride for 28 Days Causes No Effect on Serum FGF23 Concentrations in Cats
Authors: Steffen Carla , Kienzle Ellen , Dobenecker Britta
Published: November 2022

Background: FGF23 is an acknowledged parameter to assess kidney health. As chronic kidney failure is one of the most common diseases in aging cats, dietary influences on renal health warrant investigation. The purpose of this study was therefore to investigate potential correlations between dietary sodium chloride and FGF23. Methods: In a total of two trials, 11 cats were included. In the first trial, the cats were fed a complete and balanced control diet; in the second trial, sodium chloride was added (8 g/kg/DM)). Blood, urinary, feed, and faecal samples were analysed for major minerals. FGF23 and creatinine were measured in blood and urine samples. Results: Serum phosphate and FGF23 were unaffected by high sodium chloride intake, thus showing no correlation between serum FGF23 and sodium concentrations. Apparent phosphorus digestibility was significantly increased, however, by high sodium chloride intake, whereas apparent digestibility of calcium was unaffected. The present study confirms differences in FGF23 and sodium chloride interaction in cats compared with other species. Further research regarding the correlation between sodium chloride and phosphate homeostasis is warranted.
Evaluation of In Vitro Activity of Double-Carbapenem Combinations against KPC-2-, OXA-48- and NDM-Producing Escherichia coli and Klebsiella pneumoniae
Authors: Allander Lisa , Vickberg Karin , Lagerbäck Pernilla , Sandegren Linus , Tängdén Thomas
Published: November 2022

Double-carbapenem combinations have shown synergistic potential against carbapenemase-producing Enterobacterales, but data remain inconclusive. This study evaluated the activity of double-carbapenem combinations against 51 clinical KPC-2-, OXA-48-, NDM-1, and NDM-5-producing Escherichia coli and Klebsiella pneumoniae and against constructed E. coli strains harboring genes encoding KPC-2, OXA-48, or NDM-1 in an otherwise isogenic background. Two-drug combinations of ertapenem, meropenem, and doripenem were evaluated in 24 h time-lapse microscopy experiments with a subsequent spot assay and in static time-kill experiments. An enhanced effect in time-lapse microscopy experiments at 24 h and synergy in the spot assay was detected with one or more combinations against 4/14 KPC-2-, 17/17 OXA-48-, 2/17 NDM-, and 1/3 NDM-1+OXA-48-producing clinical isolates. Synergy rates were higher against meropenem- and doripenem-susceptible isolates and against OXA-48 producers. NDM production was associated with significantly lower synergy rates in E. coli. In time-kill experiments with constructed KPC-2-, OXA-48- and NDM-1-producing E. coli, 24 h synergy was not observed; however, synergy at earlier time points was found against the KPC-2- and OXA-48-producing constructs. Our findings indicate that the benefit of double-carbapenem combinations against carbapenemase-producing E. coli and K. pneumoniae is limited, especially against isolates that are resistant to the constituent antibiotics and produce NDM.
Formulation, Characterization and Evaluation of Innovative O/W Emulsions Containing Curcumin Derivatives with Enhanced Antioxidant Properties
Authors: Dalla Evdokia , Koumentakou Ioanna , Bikiaris Nikolaos , Balla Evangelia , Lykidou Smaro , Nikolaidis Nikolaos
Published: November 2022

In the present study, a series of semisolid Oil in Water (O/W) emulsions containing different Curcumin (Cur) derivatives (Cur powder, Cur extract and Cur complexed with β-cyclodextrin) in varying concentrations, were prepared. Initially, Dynamic Light Scattering (DLS), microscopy, pH and viscosity measurements were performed to evaluate their stability over time. Moreover, the effect of the active cosmetic substances on the Sun Protection Factor (SPF), antimicrobial and antioxidant properties of the prepared emulsions was investigated. It was observed that emulsions containing Cur extract and Cur β-cyclodextrin complex presented great viscosity and pH stability for up to 90 days of storage contrary to the emulsions containing Cur powder which showed unstable behavior due to the formation of agglomerates. All samples presented SPF values between 2.6 and 3.2. The emulsions with Cur in all forms exhibited high antioxidant activity, whereas the emulsion containing Cur β-cyclodextrin complex presented the highest value. Despite their improved stability and antioxidant activity, the emulsions containing Cur extract and Cur-β-cyclodextrin exhibited a low percentage of antimicrobial activity against E. coli and Staphylococcus bacteria. Instead, the emulsions containing Cur powder presented a reduction rate over 90 % against E. coli and Staphylococcus colonies.
Maternal Calorie Restriction Induces a Transcriptional Cytoprotective Response in Embryonic Liver Partially Dependent on Nrf2
Authors: Habeos George I. , Filippopoulou Fotini , Habeos Evagelia E. , Kalaitzopoulou Electra , Skipitari Marianna , Papadea Polyxeni , Lagoumintzis George , Niarchos Athanasios , Georgiou Christos D. , Chartoumpekis Dionysios V.
Published: November 2022

Background: Calorie restriction is known to enhance Nrf2 signaling and longevity in adult mice, partially by reducing reactive oxygen species, but calorie restriction during pregnancy leads to intrauterine growth retardation. The latter is associated with fetal reprogramming leading to increased incidence of obesity, metabolic syndrome and diabetes in adult life. Transcription factor Nrf2 is a central regulator of the antioxidant response and its crosstalk with metabolic pathways is emerging. We hypothesized that the Nrf2 pathway is induced in embryos during calorie restriction in pregnant mothers. Methods: From gestational day 10 up to day 16, 50% of the necessary mouse diet was provided to Nrf2 heterozygous pregnant females with fathers being of the same genotype. Embryos were harvested at the end of gestational day 16 and fetal liver was used for qRT-PCR and assessment of oxidative stress (OS). Results: Intrauterine calorie restriction led to upregulation of mRNA expression of antioxidant genes (Nqo1, Gsta1, Gsta4) and of genes related to integrated stress response (Chac1, Ddit3) in WT embryos. The expression of a key gluconeogenic (G6pase) and two lipogenic genes (Acacb, Fasn) was repressed in calorie-restricted embryos. In Nrf2 knockout embryos, the induction of Nqo1 and Gsta1 genes was abrogated while that of Gsta4 was preserved, indicating an at least partially Nrf2-dependent induction of antioxidant genes after in utero calorie restriction. Measures of OS showed no difference (superoxide radical and malondialdehyde) or a small decrease (thiobarbituric reactive substances) in calorie-restricted WT embryos. Conclusions: Calorie restriction during pregnancy elicits the transcriptional induction of cytoprotective/antioxidant genes in the fetal liver, which is at least partially Nrf2-dependent, with a physiological significance that warrants further investigation.
Effects of Different Dietary β-Glucan Levels on Antioxidant Capacity and Immunity, Gut Microbiota and Transcriptome Responses of White Shrimp (Litopenaeus vannamei) under Low Salinity
Authors: Qiao Yanbing , Zhou Li , Qu Yayu , Lu Kunyu , Han Fenglu , Li Erchao
Published: November 2022

β-Glucan could significantly improve the antioxidant capacity of aquatic animals. The effects of different dietary levels (0 (control), 0.05, 0.1, 0.2 or 0.4%) of β-glucan on the growth, survival, antioxidant capacity, immunity, intestinal microbiota and transcriptional responses of Litopenaeus vannamei under low salinity (≤3) were investigated. The dietary growth trial lasted 35 days (initial shrimp 0.26 ± 0.01 g). The results indicated that the growth performance of the 0.1% and 0.2% groups was significantly better than that of the control group. A second-order polynomial regression analysis of growth performance against dietary β-glucan indicated that the optimal dietary β-glucan level was 0.2% of dry matter. The digestive enzyme activity of the hepatopancreas was enhanced with increasing β-glucan levels. The antioxidant and nonspecific immunity capacities of the hepatopancreas were also enhanced in the 0.1% group. The α-diversity index analysis of the intestinal microbiota showed that the intestinal microbial richness of L. vannamei increased in the 0.1% group. The relative abundance of Proteobacteria decreased in the 0.1% group compared with the control group. The transcriptome results indicate that the prebiotic mechanisms of β-glucan include upregulating the expression of nonspecific immune genes and osmoregulation genes and activating KEGG pathways associated with carbohydrate metabolism under low-salinity stress. These results suggested that dietary supplementation with β-glucan markedly increased growth performance and alleviated the negative effects of low-salinity stress by contributing to the activity of biochemical enzymes and enriching carbohydrate metabolism in L. vannamei.
Radiation Type- and Dose-Specific Transcriptional Responses across Healthy and Diseased Mammalian Tissues
Authors: Sagkrioti Eftychia , Biz Gökay Mehmet , Takan Işıl , Asfa Seyedehsadaf , Nikitaki Zacharenia , Zanni Vassiliki , Kars Rumeysa Hanife , Hellweg Christine E. , Azzam Edouard I. , Logotheti Stella , Pavlopoulou Athanasia , Georgakilas Alexandros G.
Published: November 2022

Ionizing radiation (IR) is a genuine genotoxic agent and a major modality in cancer treatment. IR disrupts DNA sequences and exerts mutagenic and/or cytotoxic properties that not only alter critical cellular functions but also impact tissues proximal and distal to the irradiated site. Unveiling the molecular events governing the diverse effects of IR at the cellular and organismal levels is relevant for both radiotherapy and radiation protection. Herein, we address changes in the expression of mammalian genes induced after the exposure of a wide range of tissues to various radiation types with distinct biophysical characteristics. First, we constructed a publicly available database, termed RadBioBase, which will be updated at regular intervals. RadBioBase includes comprehensive transcriptomes of mammalian cells across healthy and diseased tissues that respond to a range of radiation types and doses. Pertinent information was derived from a hybrid analysis based on stringent literature mining and transcriptomic studies. An integrative bioinformatics methodology, including functional enrichment analysis and machine learning techniques, was employed to unveil the characteristic biological pathways related to specific radiation types and their association with various diseases. We found that the effects of high linear energy transfer (LET) radiation on cell transcriptomes significantly differ from those caused by low LET and are consistent with immunomodulation, inflammation, oxidative stress responses and cell death. The transcriptome changes also depend on the dose since low doses up to 0.5 Gy are related with cytokine cascades, while higher doses with ROS metabolism. We additionally identified distinct gene signatures for different types of radiation. Overall, our data suggest that different radiation types and doses can trigger distinct trajectories of cell-intrinsic and cell-extrinsic pathways that hold promise to be manipulated toward improving radiotherapy efficiency and reducing systemic radiotoxicities.
Investigation of Acoustic Emission of Cracks in Rails under Loading Close to Operational
Authors: Barat Vera , Marchenkov Artem , Ushanov Sergey , Bardakov Vladimir , Elizarov Sergey
Published: November 2022

The paper is devoted to the study of the possibility of detecting cracks in railway rails by the acoustic emission (AE) method. An experimental study of AE signals under cyclic compression loading of rail fragments, which simulates the rail operating load, has been carried out. Fragments of rails without defects, as well as fragments containing pre-grown fatigue cracks, were studied. It was found that AE signals generated by a rail with a crack have higher activity compared to signals from defect-free specimens. It is shown that the AE signals during the loading of defect-free specimens have a short duration and low amplitude and may be caused by the deformation of non-metallic inclusions. The crack presence leads to an increase in the AE hits rate and changes the nature of the distribution of the AE hits amplitudes. It is shown that the crack location has no effect on the reliability of its detection by the AE method. Criteria of crack detection by AE testing are offered as a result of this study.
Archaeometric Surveys of the Artifacts from the Archaeological Site of Baro Zavelea, Comacchio (Ferrara, Italy)
Authors: Marrocchino Elena , Telloli Chiara , Tessari Umberto , Cesarano Mario , Bruni Marco , Vaccaro Carmela
Published: November 2022

This work is part of a project of the Superintendence of Archaeology, Fine Arts, and Landscape for the enhancement of the widespread archaeological heritage of the Po delta area. Excavation activities, carried out in 2015, allowed the sampling of the stratigraphic elements and artifacts of the archaeological site of the lighthouse tower of Baro Zavelea, municipality of Comacchio (Ferrara, northeast Italy). In this work, the geochemical characterization of sediments and building materials was conducted using granulometric analyses, X-ray fluorescence analysis, and calcimetry on different types of samples, including sands, clays, mortars, and bricks, with the scope to better characterize all of the different types of sediments collected. This multidisciplinary approach allowed the diagnostic and evaluation of the state of conservation of Baro Zavalea. Granulometric analyses highlighted the fact that depositional environments were of very different natures: fluvial environments and paleo–alveo environments. In addition, XRF analysis allowed the discrimination of different clay samples, some from basins poor in carbonates, while, for the construction of the bricks of the second wall structure, clays rich in carbonate were chosen to add lightness to the structure.
Improved MUSIC Method against Range Dimension Deceptive Jamming Based on FDA-MIMO
Authors: Chen Yang , Tian Bo , Wang Chunyang , Gong Jian , Zhao Yingjian
Published: November 2022

Frequency diverse array-multiple-input multiple-output (FDA-MIMO) radar makes it transmit beam range–angle two-dimensional freedom by attaching a small frequency offset increment between the array elements. With the widespread use of digital radio frequency memory (DRFM) technology, it is able to delay the false target to any range bin and doppler unit by delaying and forwarding the radar transmits signals, resulting in the deceptive jamming effect of range dimension. To this end, based on the research of the existing main-lobe deceptive interference methods, this paper proposes a new improved MUSIC method based on the FDA-MIMO radar against deceptive jamming in the range dimension. Firstly, the range information of target and jamming is determined through spatial spectrum search by using the mismatch of jamming in the airspace position and range bin position, and then the process of range dimension deceptive jamming suppression is given. The simulation analysis results show that the proposed anti-jamming method can effectively mitigate against range dimension deceptive jamming.
Research on Frost Heaving Distribution of Seepage Stratum in Tunnel Construction Using Horizontal Freezing Technique
Authors: Li Mengkai , Cai Haibing , Liu Zheng , Pang Changqiang , Hong Rongbao
Published: November 2022

During the horizontal freezing construction of a subway tunnel, the delay of the closure of the frozen wall occurs frequently due to the existence of groundwater seepage, which can be directly reflected by a freezing temperature field. Accordingly, the distribution of ground surface frost heaving displacement under seepage conditions will be different from that under hydrostatic conditions. In view of this, this paper uses COMSOL to realize the hydro–thermal coupling in frozen stratum under seepage conditions, then, the frost heaving distribution of seepage stratum in tunnel construction using horizontal freezing technique is researched considering the ice–water phase transition and orthotropic deformation characteristics of frozen–thawed soil by ABAQUS. The results show that the expansion speed of upstream frozen wall is obviously slower than that of the downstream frozen wall, and the freezing temperature field is symmetrical along the seepage direction. In addition, the ground frost heaving displacement field is asymmetrically distributed along the tunnel center line, which is manifested in that the vertical frost heaving displacement of the upstream stratum is less than that of the downstream stratum. The vertical frost heaving displacement of the ground surface decreases with the increase in tunnel buried depth, but the position of the maximum value remains unchanged as the tunnel buried depth increases. The numerical simulation method established in this paper can provide a theoretical basis and design reference for the construction of a subway tunnel in a water-rich stratum under different seepage using the artificial freezing technique.
Identification and Classification of Defects in PE Gas Pipelines Based on VGG16
Authors: Wang Yang , Fu Qiankun , Lin Nan , Lan Huiqing , Zhang Hao , Ergesh Toktonur
Published: November 2022

For the problem of classification and identification of defects in polyethylene (PE) gas pipelines, this paper firstly performs preliminary screening of the acquired images and acquisition efficiency of defective image acquisition was improved. Images of defective PE gas pipelines were pre-processed. Then, edge detection of the defective images was performed using the improved Sobel algorithm and an adaptive threshold segmentation method was applied to segment the defects in the pipeline images. Finally, the defect images were morphologically processed to obtain binary images. The obtained binary images were applied with VGG16 to complete the training of the defect classifier. The experimental findings show that in the TensorFlow API environment, the test set’s highest accuracy reached 97%, which can achieve the identification of defect types of underground PE gas transmission pipelines.
Factors Influencing the Stability of a Slope Containing a Coal Seam in a Goaf
Authors: Han Chunpeng , Du Chao , Zu Fajin , Gao Jianhui
Published: November 2022

Our aim in this study was to analyze the major and minor factors affecting the stability of a slope containing a coal seam in a goaf. Based on engineering experience, we first identified nine factors that may affect slope stability, of which we determined eight that may substantially affect slope stability through a single-factor numerical simulation analysis. Then, we arranged 27 groups of numerical simulation tests with eight factors and three levels with the orthogonal test method, and we determined the ranking of the major and minor influencing factors through a range of variance analyses. The results showed that the influence of each factor was ranked as the roadway width > coal seam position > slope gradient > coal seam thickness > coal seam internal friction angle > coal seam cohesion > coal seam dip angle. Among these, the roadway width, coal seam position, and slope gradient were the major factors affecting slope stability; coal seam thickness, coal seam internal friction angle, coal seam cohesion, and coal seam dip angle were the minor factors. In this study, we combined the goaf and slope containing the coal seam, and we couple analyzed the factors influencing the stability of the slope containing the coal seam in the goaf. Our findings provide a scientific basis for the treatment and protection of slopes containing coal seams in goafs in the future and have a practical engineering importance for the analysis of the excavation stability of road-cutting slopes in goafs.