bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Evaluation of a Clinical Manual on Errorless Learning in People with Dementia
Authors: de Werd Maartje M.E. , Boelen Daniëlle , Olde Rikkert Marcel G.M. , Kessels Roy P.C.
Published: May 2015

Various studies have shown the efficacy of errorless learning (EL) in teaching patients with dementia a wide variety of skills and everyday tasks, with some studies showing beneficial effects and others reporting limited or no advantage. However, EL procedures vary greatly and, to date, no clinical guidelines or manuals are available. Here, we present a nationwide survey exploring the interest in, and feasibility of, EL in dementia care in The Netherlands. Based on the survey results and available evidence in the literature, we subsequently drafted an EL manual and had this concept manual evaluated in a Delphi round using the AGREE instrument. Forty-five health professionals associated with 22 dementia care facilities in The Netherlands, including those survey respondents who had piloted an EL intervention in accordance with the concept manual and an eight-strong expert panel representing various disciplines, deemed EL to be meaningful and feasible for use in dementia care and their residential facilities. Although our manual was favourably received, future studies are required to examine how EL can best be implemented in clinical practice and to determine the optimal outcome measures and quality indicators to reliably evaluate intervention outcomes and to consider the cost-effectiveness of the approach.
Alemtuzumab Induced Thyroid Disease in Multiple Sclerosis: A Review and Approach to Management
Authors: Mahzari Moeber , Arnaout Amel , Freedman Mark S.
Published: May 2015

Alemtuzumab, an anti-CD52 monoclonal antibody, was recently approved for treatment of MS in Canada, having shown to significantly reduce relapses and disability in patients, particularly those who relapsed despite first line treatment. Offsetting its benefit however, is the development of novel secondary autoimmune disease, particularly affecting the thyroid gland in up to 36% of patients. The incidence of Alemtuzumab induced thyroid dysfunction (AITD) will likely rise as alemtuzumab becomes more widely used for treating MS. We review the clinical and investigational cues that help delineate the aetiology and management of thyrotoxicosis and hypothyroidism in ATID. AITD can be easily managed and we present a simple approach for its evaluation and management by neurologists that should be implemented prior to considering a referral to an internist or endocrinologist for further opinion or treatment.
Learning “To Be” Kinyarwanda in Postgenocide Rwanda: Immersion, Iteration, and Reflexivity in Times of Transition
Authors: Ingelaere Bert
Published: May 2015

The research activity generating data in times of transition is subject to politicization and needs to deal with widespread distrust due to the legacy of violence or atrocity. This article discusses the main principles of a research design that took into account these hindrances by making prudence its basic tenet. The objective was to generate understanding of the functioning of Rwanda’sgacacacourt process through a heightened awareness in data collection. In doing so, this article calls attention to the importance of a reflective and adaptive research process in times of transitional justice. Two research principles are discussed in detail: immersion and iteration. The latter were adopted to facilitate the generation of context-specific knowledge on both breadth and depth of the transitional justice process. This article demonstrates how a pragmatic stance that draws on a variety of epistemologies and methodological approaches facilitates data collection as well as navigation of the field of study. It will be argued that data collection and the activity of navigating the field while collecting data reciprocally produce knowledge.
Evaluation Indexes of Military Hospitals From the Experts’ Perspective: A Qualitative Study
Authors: Ameryoun Ahmad , Haghdoost Ali Akbar , Zaboli Rouhollah , Mirzaei Tayebeh , Tofighi Shahram , Shamsi Mohammad Amin
Published: May 2015

Given the importance of evaluation in an organization and considering the objectives and missions of military hospitals, we aimed to extract some indexes (in addition to common evaluation indexes) for use in evaluating military hospitals. This was an applied-type qualitative study. The participants were 15 health experts who were first chosen by a purposeful sampling, which was then continued by theoretical sampling. The data obtained were analyzed by using MAXQDA11 software and the content analysis method. After 290 obtained codes were analyzed, 17 indexes in 6 domains were extracted, including capacity development for crisis periods, equipment and facilities, training and research, passive defense, treatment, and services, from which 8 indexes were related to capacity development for crisis periods and equipment and facilities (4 indexes each), 3 indexes were related to services, and 6 indexes were related to training and research, passive defense, and treatment (2 indexes each). The results of the present research, as a supplement to current evaluation methods such as accreditation, can be used for the comprehensive evaluation of military hospitals. (Disaster Med Public Health Preparedness. 2015;9:409–414)
Assessment of Residual Moisture and Maintenance of Sterility in Surgical Instrument Sets after Sterilization
Authors: Fayard Camille , Lambert Christophe , Guimier-Pingault Catherine , Levast Marion , Germi Raphaelle
Published: May 2015

Good sterilization practices include discarding items containing residual moisture after steam sterilization. In this small laboratory study, however, the presence of residual water did not appear to compromise the sterility of surgical instruments in 2 commonly used types of packaging during routine storage after steam sterilization.

Infect Control Hosp Epidemiol 2015;36(8):990–992
Practices to Reduce Surgical Site Infections Among Women Undergoing Cesarean Section: A Review
Authors: McKibben Rebeccah A. , Pitts Samantha I. , Suarez-Cuervo Catalina , Perl Trish M. , Bass Eric B.
Published: May 2015

OBJECTIVE

Surgical site infections (SSIs) are a leading cause of morbidity and mortality among women undergoing cesarean section (C-section), a common procedure in North America. While risk factors for SSI are often modifiable, wide variation in clinical practice exists. With this review, we provide a comprehensive overview of the results and quality of systematic reviews and meta-analyses on interventions to reduce surgical site infections among women undergoing C-section.

METHODS

We searched PubMed and the Cochrane Database of Systematic Reviews for systematic reviews and meta-analyses published between January 2000 and May 2014 on interventions to reduce the occurrence of SSIs (incisional infections and endometritis), among women undergoing C-section. We extracted data on the interventions, outcomes, and strength of evidence as determined by the original article authors, and assessed the quality of each article based on a modified Assessment of Multiple Systematic Reviews tool.

RESULTS

A total of 30 review articles met inclusion criteria and were reviewed. Among these articles, 77 distinct interventions were evaluated: 29% were supported with strong evidence as assessed by the original article authors, and 83% of the reviews articles were classified as good quality based on our assessment. Ten interventions were classified as being effective in reducing SSI with strong evidence in a good-quality article, including preoperative vaginal cleansing, the use of perioperative antibiotic prophylaxis, and several surgical techniques.

CONCLUSION

Efforts to reduce SSI rates among women undergoing C-section should include interventions such as preoperative vaginal cleansing and the use of perioperative antibiotics because compelling evidence exists to support their effectiveness.

Infect Control Hosp Epidemiol 2015;36(8):915–921
Turbulence attenuation in particle-laden flow in smooth and rough channels
Authors: Vreman A. W.
Published: May 2015

Results of point-particle direct numerical simulations of downward gas–solid flow in smooth and rough vertical channels are presented. Two-way coupling and inter-particle collisions are included. The rough walls are shaped as fixed layers of tiny spherical particles with diameter much smaller than the viscous wall unit. The turbulence attenuation induced by the free solid particles in the gas flow is shown to be enhanced with increasing wall roughness. The so-called feedback force, the force exerted by the free particles on the gas, is decomposed into three contributions: the domain average of the mean feedback force, the non-uniform part of the mean feedback force and the fluctuating part of the feedback force. Since the non-uniformity of the mean feedback force increases with wall roughness, the effect of the non-uniform part of the mean feedback force is investigated in detail. For both smooth and rough walls, the non-uniform part of the mean feedback force is shown to contribute significantly to the particle-induced turbulence attenuation.
Alliance network size, partner diversity, and knowledge creation in small biotech firms
Authors: Yoon Woojin , Lee Diane Y. , Song Jaeyong
Published: May 2015

This paper investigates the effects on knowledge creation of network size and partner diversity formed through alliance relationships. These effects are tested empirically in the biotech industry setting, which is representative of industries that emphasize external collaboration. Using patent count as a proxy of knowledge creation, Poisson regression was employed to test our predictions empirically. The statistical results show an invertedU-shaped relationship between network size and knowledge creation. In addition, a negative relationship was observed between partner diversity and knowledge creation. This research suggests that small biotech firms should strive to achieve a balanced network size. Knowledge creation is better promoted in these firms through alliances with firms of similar organizational type. The value of this research lies in the fact that it provides new insight into properties of alliance networks by highlighting potentially negative consequences of having an oversized alliance network and partner diversity.
Ten Years of The Journal of Smoking Cessation
Authors: Bittoun Renee
Published: May 2015

The advent and success of a new Journal seems to be dependent on its originality and a readership that needs and appreciates the content. It still is surprising to me personally that the Journal of Smoking Cessation (JoSC) has succeeded to such a degree since its inception 10 years ago. When I thought about a Journal for our group of the Australian Association of Smoking Cessation Professionals (AASCP), the publishers at Australian Academic Press asked whether there surely was such a journal already and that another might be redundant. When I assured them that there was no such Journal, we embarked upon an enterprise which now has great international interest and credibility. With a small startup grant from the Australian Government I was able to encourage and recruit old friends and colleagues from around the world who were all kind enough to give their names in good faith to an Editorial Board that remains the same today. Their good names and reputations greatly enhanced the credibility of the Journal and I cannot thank them enough for their faith in the Journal and its future. In particular thanks go to Michelle DiGiacomo for volunteering and continuing to volunteer an inordinate amount of time and energy supporting this endeavour.
Holocene landscape reconstruction of the Wadden Sea area between Marsdiep and Weser
Authors: Vos P.C. , Knol E.
Published: May 2015

This paper describes the background of five palaeogeographical maps between the Marsdiep and the Weser River, and discusses the natural and anthropogenic processes driving the coastal changes during the last part of the Holocene. Before 2500 BC, during the first half of the Holocene, tidal basins were formed in the lower lying Pleistocene valley system as a result of the Holocene sea-level rise. The tidal basins were filled during the second half of the Holocene and on the deposits from the Pleistocene in the hinterland large coastal peat bogs developed. These peat bogs were vulnerable and sensitive to marine ingressions when the peat surface subsided due to drainage, compaction and erosion. During the Subatlantic (450 BC to present), the different ingression systems in the coastal area between Marsdiep and Weser had their own histories in timing and evolution. The ingressions were naturally caused by lateral migration of coastal barrier and tidal-inlet systems or by changes in the natural drainage system in the hinterland. From the Late Iron Age onwards, humans started to be the major cause of ingressions. By reclaiming and cultivating the seaward margins of coastal peat bogs, these areas subsided significantly and were flooded by high storm surges. When coastal areas were embanked during the historical period, the situation for the lower lying peat lands became more dramatic. When the sea dikes breached, the peat land was flooded, leading to casualties and huge material damages and loss of land. Drowning of the peat lands of the Jade and Dollard in the 14th and 15th centuries are examples of such catastrophes.
Regular Voters, Marginal Voters and the Electoral Effects of Turnout
Authors: Fowler Anthony
Published: May 2015

How do marginal voters differ from regular voters? This article develops a method for comparing the partisan preferences of regular voters to those marginal voters whose turnout decisions are influenced by exogenous factors and applies it to two sources of variation in turnout in the United States—weather and election timing. In both cases, marginal voters are over 20 percentage points more supportive of the Democratic Party than regular voters—a significant divide. The findings suggest that the expansion or contraction of the electorate can have important consequences. Moreover, the findings suggest that election results do not always reflect the preferences of the citizenry, because the marginal citizens who may stay home have systematically different preferences than those who participate. Finally, the methods developed in the article may enable future researchers to compare regular and marginal voters on many different dimensions and in many different electoral settings.
Contents of total fat, fatty acids, starch, sugars and dietary fibre in Swedish market basket diets
Authors: Becker W. , Eriksson A. , Haglund M. , Wretling S.
Published: May 2015

The typical dietary supply of total fat, fatty acids, starch, sugars, polyols and dietary fibre in Sweden was assessed from analyses of market baskets (MB) purchased in 2005 and 2010. MB were based on food balance sheets, with each basket comprising about 130 foods, which represented more than 90 % of annual dietary supply. Foods were divided into ten to twelve categories. In 2010, total fat contributed 34 % of energy (E%), SFA 14·3 E%, MUFA 12·8 E%, PUFA 4·6 E%,n-6 fatty acids 3·6 E%,n-3 fatty acids 1·0 E% andtrans-fatty acids (TFA) 0·5 E%. Glycaemic carbohydrates contributed 47 E%, monosaccharides 9 E%, sucrose 11 E%, disaccharides 15 E% and total sugars 24 E%. Added sugars contributed about 15 E%. Dietary fibre content was about 1·7 g/MJ in the 2010 MB. Compared with the 2005 MB, the dietary supply of TFA and dietary fibre was lower, otherwise differences were small. The present MB survey shows that the content of SFA and added sugars was higher than the current Nordic Nutrition Recommendations, while the content of PUFA and especially dietary fibre was lower. TFA levels decreased and dietary supply was well below the recommendations of the WHO. These results emphasise a focus on quality and food sources of fat and carbohydrates, limiting foods rich in SFA and added sugars and replacing them with foods rich in dietary fibre andcis-unsaturated fatty acids.
Adipose tissue hypoxia and low-grade inflammation: a possible mechanism for ethanol-related glucose intolerance?
Authors: He Zhen , Li Min , Zheng Dongmei , Chen Qing , Liu Wenwen , Feng Li
Published: May 2015

The exact mechanism of ethanol's effects on glucose tolerance has not been well determined. The present study focuses for the first time on hypoxia and low-grade inflammation in adipose tissue (AT). In thein vivoexperiments, twenty-four male Wistar rats were randomly allocated into control and ethanol feeding groups. Ethanol-treated rats received edible ethanol once a day at a total dosage of 5 g/kg per d, and the controls received distilled water. Ethanol volumes were adjusted every week. At the end of 8 weeks, we carried out an oral glucose tolerance test. Blood and AT were collected for measuring hypoxia-inducible factor-1α (HIF-1α), GLUT1, TNF-α, IL-6, leptin and vascular endothelial growth factor (VEGF). In thein vitroexperiments, differentiated OP9 adipocytes were incubated with 100 mmof ethanol for 48 h; the media and cells were then collected for measuring HIF-1α, GLUT1, TNF-α and IL-6. The results showed that long-term ethanol consumption impaired glucose tolerance in rats. Ethanol consumption had little influence on body weight, but both epididymal and perirenal AT were markedly enlarged in the ethanol-treated rats as compared to the controls. Visceral adipose tissue (VAT) had accumulated, and the protein levels of HIF-1α and GLUT1, the indicators of hypoxia in rat epididymal AT and OP9 adipocytes, were elevated. Secondary to the AT hypoxia, the levels of inflammation-related adipokines, such as TNF-α, IL-6, leptin and VEGF, were increased. Based on these findings, we conclude that VAT hypoxia and low-grade inflammation might be a new mechanism in the treatment of ethanol-related diabetes.
Response ofAphidius colemanito aphid sex pheromone varies depending on plant synergy and prior experience
Authors: Fernández-Grandon G.M. , Poppy G.M.
Published: May 2015

A critical stage in the success of a parasitoid is the ability to locate a host within its habitat. It is hypothesized that a series of olfactory cues may be involved in altering the parasitoid's movement patterns at this stage of foraging. This paper focuses specifically on host habitat location and host location and the olfactory stimuli necessary to mediate the transition between these stages. Firstly, we confirm the ability of the parasitoidAphidius colemanito detect the aphid sex pheromone at an electrophysiological level. Following this we investigate the effect of the sex pheromone component (4aS,7S,7aR)-nepetalactone on the movement patterns ofA. colemaniand its retention within an area. The key findings of this work are thatA. colemaniis able to detect the sex pheromone components, that parasitoid retention is increased by a synergy of nepetalactone and other host-associated cues and that foraging patterns are augmented by the presence of nepetalactone or experience associated with nepetalactone.
DNA-based identifications reveal multiple introductions of the vegetable leafminer Liriomyza sativae (Diptera: Agromyzidae) into the Torres Strait Islands and Papua New Guinea
Authors: Blacket M.J. , Rice A.D. , Semeraro L. , Malipatil M.B.
Published: May 2015

Leafmining flies (Diptera: Agromyzidae) can be serious economic pests of horticultural crops. Some genera such as Liriomyza are particularly problematic with numerous species, some of which are highly polyphagous (wide host range), which can only be confidently identified morphologically from adult males. In our study, DNA barcoding was employed to establish new locality records of the vegetable leafminer fly, Liriomyza sativae, from the islands of Torres Strait (Queensland, Australia) and the central highlands of Papua New Guinea (PNG). These records represent significant range extensions of this highly invasive plant pest. Specimens of immature leafminers (from leaf mines) were collected over a 5-year period during routine plant health surveys in ethanol or on FTA® filter paper cards, both methods proved effective at preserving and transporting insect DNA under tropical conditions, with FTA cards possessing some additional logistical benefits. Specimens were identified through sequencing two sections of the cytochrome oxidase I gene and the utility of each was assessed for the identification of species and intra-specific genetic lineages. Our study indicates that multiple haplotypes of L. sativae occur in PNG, while a different haplotype is present in the Torres Strait, with genetic regionalization between these areas apart from a single possible instance – one haplotype ‘S.7’ appears to be common between these two regions – interestingly this has also been the most common haplotype detected in previous studies of invasive L. sativae populations. The DNA barcoding methods employed here not only identified multiple introductions of L. sativae, but also appear generally applicable to the identification of other agromyzid leafminers (Phytomyzinae and Agromyzinae) and should decrease the likelihood of potentially co-amplifying internal hymenopteran parasitoids. Currently, L. sativae is still not recorded from the Australian mainland; however, further sampling of leafminer flies from Northern Australia and surrounding areas is required, as surveillance for possible Liriomyza incursions, as well as to characterize endemic species with which Liriomyza species might be confused.
DNA barcoding of common soft scales (Hemiptera: Coccoidea: Coccidae) in China
Authors: Wang X.-B. , Deng J. , Zhang J.-T. , Zhou Q.-S. , Zhang Y.-Z. , Wu S.-A.
Published: May 2015

The soft scales (Hemiptera: Coccoidea: Coccidae) are a group of sap-sucking plant parasites, many of which are notorious agricultural pests. The quarantine and economic importance of soft scales necessitates rapid and reliable identification of these taxa. Nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene (barcoding region) and 28S rDNA were generated from 340 individuals of 36 common soft scales in China. Distance-based [(best match, Automated Barcode Gap Discovery (ABGD)], tree-based (neighbor-joining, Bayesian inference), Klee diagrams, and general mixed Yule coalescent (GMYC) models were used to evaluate barcoding success rates in the data set. Best match showed that COI and 28S sequences could provide 100 and 95.52% correct identification, respectively. The average interspecific divergences were 19.81% for COI data and 20.38% for 28S data, and mean intraspecific divergences were 0.56 and 0.07%, respectively. For COI data, multiple methods (ABGD, Klee, and tree-based methods) resulted in general congruence with morphological identifications. However, GMYC analysis tended to provide more molecular operational taxonomic units (MOTUs). Twelve MOTUs derived from five morphospecies (Rhodococcus sariuoni, Pulvinaria vitis, Pulvinaria aurantii, Parasaissetia nigra, and Ceroplastes rubens) were observed using the GMYC approach. In addition, tree-based methods showed that 28S sequences could be used for species-level identification (except for Ceroplastes ceriferus – Ceroplastes pseudoceriferus), even with low genetic variation (<1%). This report demonstrates the robustness of DNA barcoding for species discrimination of soft scales with two molecular markers (COI and 28S) and provides a reliable barcode library and rapid diagnostic tool for common soft scales in China.
IMPACT OF NUTRITIONAL PERCEPTIONS OF TRADITIONAL AFRICAN VEGETABLES ON FARM HOUSEHOLD PRODUCTION DECISIONS: A CASE STUDY OF SMALLHOLDERS IN TANZANIA
Authors: AFARI-SEFA V. , RAJENDRAN S. , KESSY R. F. , KARANJA D. K. , MUSEBE R. , SAMALI S. , MAKARANGA M.
Published: May 2015

Recent years have seen increasing political interest and growing public health awareness and advocacy for diversifying diets into highly nutritious traditional vegetables, fruits and other nutrition-sensitive crops as a more viable approach to mitigate the growing scourge of malnutrition due to unhealthy and imbalanced diets. These foods contribute essential micronutrients, vitamins, antioxidants, and other health-related phytochemicals to staple-based diets and their consumption is crucial for the attainment of several Millennium Development Goals. Despite their nutritional benefits and the high farm gate values per unit of land, the production and marketing of traditional vegetables from Tanzania and other countries in sub-Saharan Africa are constrained by factors such as poor quality seeds, lack of appropriate market information and support systems, and lack of consumer awareness of their nutritional importance. This paper investigates the determinants and pathways for smallholder participation in traditional African vegetable production and identifies entry points for farmers to increase traditional vegetable production by linking nutritional awareness and promotion with potential high value markets. A primary survey of 181 traditional vegetable growers from five regions of Tanzania indicates that perceptions about the nutritional value of traditional African vegetables are a main driver of household production decisions in the sector. The results of this study provide evidence that farmers tend to grow more African traditional vegetables as compared to other crops based on their increased level of perception towards nutritional value of traditional vegetables along with other factors such as their market value, timely availability of quality certified seeds, willingness to invest in labour, required training for women and better access to credits. Farm size negatively affects growing traditional vegetables, implying that on a comparative basis, smallholders tend to grow more traditional vegetables than larger-farm operators. Thus, more attention should be given to reducing production and its associated transaction costs by ensuring timely access to quality certified seeds, ensuring optimal use of inputs and increasing labour productivity, particularly for smallholders.
Effects of soil zinc availability, nitrogen fertilizer rate and zinc fertilizer application method on zinc biofortification of rice
Authors: GUO J. X. , FENG X. M. , HU X. Y. , TIAN G. L. , LING N. , WANG J. H. , SHEN Q. R. , GUO S. W.
Published: May 2015

Rice (Oryza sativa L.) is one of the most important cereal crops in the world and a potentially important source of zinc (Zn) in the diet. The improvement of Zn content of rice is a global challenge with implications for both rice production and human health. The objective of the present study was to identify the effects of nitrogen (N) fertilizer rates and Zn application methods on Zn content of rice by evaluating rice production on native soils with different Zn availabilities in 2010/11. The results indicated that Zn application increased rice grain yield and Zn content in grains compared with the control; however, this effect was also affected by the native soil Zn availability, N fertilizer rate and Zn fertilizer application method. The native soil Zn status was the dominant factor influencing grain yield and grain Zn content in response to Zn fertilizer application. Grain Zn content ranged from 19·74 to 26·93 mg/kg under the different Zn statuses. The results also indicated that Zn application method has a significant influence on grain yield. Application of Zn fertilizer to the soil was more effective than the foliar spray on rice grain yield; however, the foliar spray resulted in a greater increase in grain Zn content when compared with soil application. Grain Zn content was affected by application method and displayed the following general trend: soil application + foliar spray > foliar spray > soil application. The experiments investigating the effect of N fertilizer rate combined with Zn application method showed a clear increase in both grain yield and Zn content as the N fertilizer level increased from 200 to 300 kg/ha. In addition, the results also indicated that N content and accumulation increased in all plant tissues, which suggests that Zn application might influence the uptake and translocation of N in rice plants. These results suggest that soil application in addition to a foliar spray of Zn should be considered as an important strategy to increase grain yield and grain Zn content of rice grown in soils with low background levels of Zn-associated diethylene triamine pentaacetate acid. Moreover, this process could be further strengthened by a high N application rate. In conclusion, these results demonstrate the potential of optimizing nutrient management using Zn fertilizer to obtain higher grain yields and higher grain Zn content in fields with low native Zn status.
Apparent digestibility, rumen fermentation and nitrogen balance in Tibetan and fine-wool sheep offered forage-concentrate diets differing in nitrogen concentration
Authors: ZHOU J. W. , MI J. D. , DEGEN A. A. , GUO X. S. , WANG H. C. , DING L. M. , QIU Q. , LONG R. J.
Published: May 2015

A comparative study of the effect of dietary nitrogen (N) content [Low: 11·0; Medium-Low (MLow): 16·7; Medium-High (Mhigh): 23·1; High: 29·2 N g/kg dry matter (DM)] on apparent digestibilities, rumen fermentation and N balance was conducted in coarse wool Tibetan sheep and Gansu Alpine fine-wool sheep at Wushaoling in the northeast of the Qinghai-Tibetan Plateau. It was hypothesized that responses would differ between breeds and that responses would favour Tibetan over fine-wool sheep at low N intakes. Eight wethers [four Tibetan sheep and four fine-wool sheep, 20–24 months old; body weight ± standard deviation was 52 ± 3·2 kg] were used in two concurrent 4 × 4 Latin square designs. Dry matter, organic matter, neutral detergent fibre and acid detergent fibre digestibilities were higher in Tibetan than fine-wool sheep when fed the Low, MLow and High N diets while N retention was higher when the animals were fed the Low and MLow N diets. Tibetan sheep had a higher rumen pH than fine-wool sheep; however, total volatile fatty acids were similar between breeds. Molar proportions of acetate were higher but propionate and butyrate lower in Tibetan than fine-wool sheep. In addition, Tibetan sheep had higher concentrations of ruminal free amino acid-N and soluble protein-N than fine-wool sheep. Plasma and saliva urea-N concentrations were higher in Tibetan than fine-wool sheep when supplied with the Low N diet. It was concluded that Tibetan sheep were better able to cope with low N feed than fine-wool sheep because of the higher N retention and higher DM and fibre digestibilities with Low and MLow diets.
Identification and characterization of immunodominant linear epitopes on the antigenic region of a serine protease in newbornTrichinellalarvae
Authors: Yang Y. , Vallée I. , Lacour S.A. , Boireau P. , Cheng S.P. , Liu M.Y.
Published: May 2015

An immunodominant serine protease ofTrichinella spiralisnamed NBL1 showed encouraging potential in early diagnosis of trichinellosis in pigs and elicited protective immune responses during infection of animals. To further define serological reagents for diagnostic use, the specific epitopes on NBL protein recognized by the antibody responses of different susceptible hosts need to be defined. The present study described comprehensive mapping of immunodominant linear epitopes in the antigenic region (NBL-C, the C-terminal part of the protein) using various serum samples obtained from three kinds of hosts – pig, wild boar and mice. We identified six peptides which were commonly recognized by sera from pigs experimentally infected withTrichinellaand pigs immunized with rNBL1-C; five and four peptides were recognized by sera from wild boars and mice infected withTrichinella,respectively. Three peptides (NBL1-6, -7 and -9) were commonly recognized by antisera in all three hosts, which share the sequence PSSGSRPTYP. We also found that one peptide (NBL1-12) was only recognized by antibodies from pigs immunized with rNBL1-C. The identification of specific epitopes targeted by the host antibody response is important both for understanding the natural response to infection and for the development of subunit vaccines and diagnostic tools for trichinellosis.
Immune responses in children infected with the pinwormEnterobius vermicularisin central Greece
Authors: Patsantara G.G. , Piperaki E.-T. , Tzoumaka-Bakoula C. , Kanariou M.G.
Published: May 2015

Previous studies have suggested an immunomodulatory and even protective role forEnterobius vermicularis, the least pathogenic human intestinal helminth. Here, in a study using haematological and serological parameters, we tested a total of 215 children from central Greece, with a mean age of 8.39, of whom 105 (48.84%) were infected withE. vermicularisand 110 (51.16%) were matched healthy controls. In particular, we analysed eosinophil counts (EO), serum eosinophil cationic protein (ECP), total and specific serum immunoglobulin E (IgE) and the ECP/EO ratio. The atopic status and the potential occurrence of clinically expressed allergic diseases were both taken into account. Eosinophils, ECP and IgE were found to be higher in infected than in uninfected children, indicating a type-2 immune response activation during infection. Atopic infected children exhibited higher IgE levels compared to non-atopic ones. EO and ECP were found to be lower in atopic children who had a history of allergic disease than in those with no such history. The type-2 oriented immune response elicited againstE. vermiculariscould contribute to a balanced activation of the immune system in the examined children. Interestingly, although the atopic children showed a stronger activation, they did not exhibit any symptoms and, moreover, there seemed to be some indication of immunosuppression in those children with a positive history of allergic disease.
Necrotising (malignant) otitis externa in the UK: a growing problem. Review of five cases and analysis of national Hospital Episode Statistics trends
Authors: Chawdhary G , Liow N , Democratis J , Whiteside O
Published: May 2015

Background:

Necrotising (malignant) otitis externa is a severe infection causing temporal bone osteomyelitis. Although rare, our experience (reported herein) shows local doubling of cases in 2013. Hospital Episodes Statistics data for England over 14 years also indicate increased incidence nationally. Specific learning points in management are also discussed.

Methods:

A retrospective review was conducted of patients admitted in 2013 to Wexham Park Hospital, Slough, UK (catchment population, 450 000). In addition, the UK Government Hospital Episodes Statistics data were interrogated.

Results:

There were five cases of necrotising (malignant) otitis externa in 2013, representing a local doubling on previous years. The mean age of patients was 82 years. All cultures grew Pseudomonas aeruginosa; no isolates were antibiotic resistant. All patients responded to systemic anti-pseudomonals on clinical, biochemical and radiological parameters. Hospital Episodes Statistics data showed a six-fold increase in the number of cases from 1999 (n = 67) to 2013 (n = 421).

Conclusion:

Our experience suggests increasing necrotising (malignant) otitis externa incidence, and retrospective analysis of Hospital Episodes Statistics data supports this observation. Necrotising (malignant) otitis externa poses challenges in management, as exemplified in our cases, requiring a high index of suspicion and early aggressive treatment to achieve cure.
Débrouillardise: certifying ‘conflict-free’ minerals in a context of regulatory pluralism in South Kivu, DR Congo
Authors: Müller-Koné Marie
Published: May 2015

This article explores the relationship between transnational governance initiatives for ‘conflict-free’ certification in the eastern provinces of the Democratic Republic of the Congo (DRC) and the regulatory pluralism one finds on the ground. Efforts in certifying artisanal gold mining are scrutinised by analysing how three different gold mining sites in the DRC's South Kivu province are governed. Most artisanal mining in the DRC is usually referred to as ‘informal’ – a term associated with non-state actors. Instead, the article introduces the idea of a mode of governing that follows the principle of ‘débrouillardise’, which combines different rule systems and state and non-state regulators. It argues that ‘conflict-free’ governance will need to improvise viaad hocagreements on the legal status of mining sites among state authorities, economic actors and international monitors. The act of declaring mining sites legal will provide for the semblance of a ‘conflict-free’ status and a unitary state system of rule, while in practice, the plurality of regulatory authority will not be reversed.
Meiofauna distribution on hard substrata in a submarine cave
Authors: Russo Riccardo , Valente Sara , Colangelo Giuseppe , Belmonte Genuario
Published: May 2015

For the first time the meiofauna of the rocky walls of a submarine cave was studied. The cave, known as il Ciolo (Strait of Otranto, south-east Italy) is a closed tunnel about 125 m long, with a maximum depth of 6 m below sea level. The meiofauna was collected from artificial panels and natural rocky walls. This double approach enabled: (1) the description of the community's initial organization (on artificial substrata), and (2), especially for Harpacticoida, its mature composition (on rocky walls), which also helped to establish spatial differences. The collected samples yielded 70 taxa in total. Harpacticoida represented the most important group of organisms in terms of both abundance and identified taxa. The meiofauna assemblage appeared not to be affected by community age, with the exception of the very early stage. The meiofauna of the cave showed assemblage differences from the entrance to the innermost positions, but not as evident as in the case of the macrobenthos. The similarity of community composition at different ages (6, 12 and 24 months) and at different positions along the cave could be the consequence of the specimens’ vagility.
Colonization of depopulated crinoids by symbionts: who comes from the bottom and who from the water column?
Authors: Mekhova E.S. , Dgebuadze P.Y. , Mikheev V.N. , Britayev T.A.
Published: May 2015

Previous experiments with the comatulid Himerometra robustipinna (Carpenter, 1881) demonstrated intensive host-to-host migration processes for almost all symbiotic species both within host aggregations and among hosts separated by several metres. The aim of this study was to check the ability of symbionts to complete long-distance migrations, by means of two in situ experiments which depopulated the crinoid host. Two different sets of field experiments were set up: exposure of depopulated crinoids (set 1) on stony ‘islands’ isolated from native crinoid assemblages by sandy substrate, and (set 2) in cages suspended in the water column. Hosts from set 1 were exposed for 1, 2, 3 and 4 weeks to assess whether substrate has an influence on the symbionts' long-distance migrations. In set 2 cages were exposed for 10–11 days, aiming to check whether symbionts were able to disperse through the water column with currents. These experiments allow the conclusion that post-settled symbionts can actively migrate among their hosts. Symbionts are able to reach their hosts by employing two different ‘transport corridors’, by drifting or swimming in water column, and by moving on the bottom. Comparison of experimental results allows the division of symbionts into two conventional groups according to the dispersal ability of their post-settled stages: (1) species able to complete long-distance migrations, (2) species unable to migrate or having limited dispersal ability. The finding of the free-living shrimp Periclimenes diversipes Kemp, 1922 in set 2 raises the question about the factors that affect such a high degree of specialization of crinoid assemblages.
Feeding behaviour of the common dolphinfishCoryphaena hippurus: older fish use more complex foraging strategies
Authors: Nunes José De Anchieta C.C. , Freitas Renato H. A. , Reis-Filho José A. , Loiola Miguel , Sampaio Cláudio L.S.
Published: May 2015

The common dolphinfish (Coryphaena hippurus) is widely distributed and represents an important part of the currently exploited fishery resources. This species’ feeding behaviour, however, is poorly understood. This study aimed to investigate the feeding behaviour of dolphinfish and the effect of body size (and the consequently aggregated learning experience) on the strategies used for capturing their prey. Observations were made from an oil platform in north-eastern Brazil. Dolphinfish length was visually estimated and classified into three size classes. The following foraging strategies were observed in our study: ‘active chasing’, ‘leaping out’, ‘swimming in circles’, ‘surf’, ‘using floating objects’ and ‘cleaning turtle carapaces’. The most frequently used foraging strategy was ‘active chasing’, suggesting a strong preference of dolphinfish in using visual cues during predation. Smaller size-classes of dolphinfish did not perform the ‘swimming in circles’ and ‘surf’ strategy, probably due to a lack of learning experience, since further social interactions could be needed in order to show this strategy. Smaller dolphinfish regularly showed the ‘using floating objects’ as a foraging strategy, probably reflecting an opportunistic behaviour. The smallest individuals were using more simple strategies, to chase smaller prey that could have different and less complex escape strategies. It indicates learning experience could play an important role into the dolphinfish ontogeny, moving towards more complex foraging strategies throughout their lives. Independently of the age class, the dolphinfish displayed a varied repertory of foraging strategies, maximizing hunting success in the open ocean, a hostile pelagic environment with a low prey availability.
Relevance of liver fat to the impact of dietary extrinsic sugars on lipid metabolism
Authors: Griffin B. A.
Published: May 2015

In contrast to the decline in mortality from many non-infectious, chronic diseases in the UK, death from liver disease has increased exponentially in men and women over the past 40 years. This is primarily because of the over consumption of alcohol, but also the increased prevalence of obesity, which is linked to early pathology through the accumulation of liver fat. Supra-physiological intakes of fructose-containing sugar can produce acute, adverse effects on lipid metabolism, and deliver excess energy that increases bodyweight and the deposition of fat in sites other than adipose tissue, including the liver. This review addresses the variable metabolic origins of liver fat, and the key importance of postprandial lipid metabolism in this respect. The effects of supra-physiological intakes of sugar are also considered in context of the real world and established threshold for the adverse effects of sugar on cardio-metabolic risk factors. The review concludes that while the average intake of sugar in the UK falls well below this critical threshold, intakes in subgroups of adults, and especially adolescents, may be cause for concern. There is also evidence to suggest that raised liver fat, acquired, in part, through an impaired removal of postprandial lipaemia, can increase sensitivity to the adverse effects of sugar at all ages.
Translocation of problem predators: is it an effective way to mitigate conflict between farmers and cheetahsAcinonyx jubatusin Botswana?
Authors: Boast Lorraine K. , Good Kyle , Klein Rebecca
Published: May 2015

The translocation of predators believed to be preying on livestock is often perceived as a more humane and desirable method of removal than lethal control. However, the survival of translocated predators and the effectiveness of translocation in reducing conflict at the removal site are often not documented. We assessed farmers’ perceptions of the efficacy of translocation at reducing livestock and game-stock losses in Botswana, and determined the post-release survival of translocated cheetahsAcinonyx jubatus, the most threatened large felid in Africa. Eighteen percent of translocated cheetahs survived 1 year (n = 11). The low survival rate was thought to be related to homing behaviour and wide-ranging movements post release. The majority of farmers who had translocated a problem predator from their farm within the 12 months prior to the study perceived that the translocation was ineffective at reducing stock losses, both in the short (59.1%) and long term (63.6%, n = 22). At least one of the monitored cheetahs continued to predate livestock after release. In light of the low survival, significant financial costs and failure to reduce stock losses, we conclude that the translocation of problem cheetahs in Botswana should no longer be conducted, and that conflict mitigation methods should focus on techniques that promote coexistence of predators and humans.
Continuing mortality of vultures in India associated with illegal veterinary use of diclofenac and a potential threat from nimesulide
Authors: Cuthbert Richard J. , Taggart Mark A. , Saini Mohini , Sharma Anil , Das Asit , Kulkarni Mandar D. , Deori Parag , Ranade Sachin , Shringarpure Rohan N. , Galligan Toby H. , Green Rhys E.
Published: May 2015

The collapse of South Asia's Gyps vulture populations is attributable to the veterinary use of the non-steroidal anti-inflammatory drug (NSAID) diclofenac. Vultures died after feeding on carcasses of recently-medicated animals. The governments of India, Nepal and Pakistan banned the veterinary use of diclofenac in 2006. We analysed results of 62 necropsies and 48 NSAID assays of liver and/or kidney for vultures of five species found dead in India between 2000 and 2012. Visceral gout and diclofenac were detected in vultures from nine states and three species: Gyps bengalensis, Gyps indicus and Gyps himalayensis. Visceral gout was found in every vulture carcass in which a measurable level of diclofenac was detected. Meloxicam, an NSAID of low toxicity to vultures, was found in two vultures and nimesulide in five vultures. Nimesulide at elevated tissue concentrations was associated with visceral gout in four of these cases, always without diclofenac, suggesting that nimesulide may have similar toxic effects to those of diclofenac. Residues of meloxicam on its own were never associated with visceral gout. The proportion of Gyps vultures found dead in the wild in India with measurable levels of diclofenac in their tissues showed a modest and non-significant decline since the ban on the veterinary use of diclofenac. The prevalence of visceral gout declined less, probably because some cases of visceral gout from 2008 onwards were associated with nimesulide rather than diclofenac. Veterinary use of nimesulide is a potential threat to the recovery of vulture populations.
Robert Mossman, Endurance and the Weddell Sea ice
Authors: Burton Robert , King John C.
Published: May 2015

Before Shackleton arrived at South Georgia aboard Endurance on 5 November 1914 he was aware that the vessel might meet bad pack-ice in the Weddell Sea. This had been forecast on the basis of climate analysis by Robert Mossman, the meteorologist on the Scottish National Antarctic Expedition (1902–1904), who was currently working at the Argentine Meteorological Office. Mossman was interested in teleconnections linking meteorological and oceanic conditions in widely separated places and had studied the links between the Weddell Sea and South America. Mossman's Antarctic data were mainly records from the Orcadas station in the South Orkneys which had operated continuously from 1903. He found a correlation between extensive pack-ice in the Weddell Sea and plentiful rain in a belt across South America that included Buenos Aires. The experiences of Endurance supported this. Modern studies of the El Niño-Southern Oscillation (ENSO) broadly confirm Mossman's conclusions.