bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of arch support doses on the center of pressure and pressure distribution of running using statistical parametric mapping
Authors: Cheng Jiale , Zeng Qing , Lai Jiaqi , Zhang Xianyi
Published: November 2022

Insoles with an arch support have been used to address biomechanical risk factors of running. However, the relationship between the dose of support and running biomechanics remains unclear. The purpose of this study was to determine the effects of changing arch support doses on the center of pressure (COP) and pressure mapping using statistical parametric mapping (SPM). Nine arch support variations (3 heights * 3 widths) and a flat insole control were tested on fifteen healthy recreational runners using a 1-m Footscan pressure plate. The medial-lateral COP (COPML) coordinates and the total COP velocity (COPVtotal) were calculated throughout the entirety of stance. One-dimensional and two-dimensional SPM were performed to assess differences between the arch support and control conditions for time series of COP variables and pressure mapping at a pixel level, respectively. Two-way ANOVAs were performed to test the main effect of the arch support height and width, and their interaction on the peak values of the COPVtotal. The results showed that the COPVtotal during the forefoot contact and forefoot push off phases was increased by arch supports, while the COP medial-lateral coordinates remained unchanged. There was a dose-response effect of the arch support height on peak values of the COPVtotal, with a higher support increasing the first and third valleys but decreasing the third peak of the COPVtotal. Meanwhile, a higher arch support height shifted the peak pressure from the medial forefoot and rearfoot to the medial arch. It is concluded that changing arch support doses, primarily the height, systematically altered the COP velocities and peak plantar pressure at a pixel level during running. When assessing subtle modifications in the arch support, the COP velocity was a more sensitive variable than COP coordinates. SPM provides a high-resolution view of pressure comparisons, and is recommended for future insole/footwear investigations to better understand the underlying mechanisms and improve insole design.
The RNA-binding protein RBM24 regulates lipid metabolism and SLC7A11 mRNA stability to modulate ferroptosis and inflammatory response
Authors: Zhang Jin , Kong Xiangmudong , Sun Wenqiang , Wang Leyi , Shen Tong , Chen Mingyi , Chen Xinbin
Published: November 2022

Lipids play a critical role in many cellular processes by serving as structural components of cell membranes or functioning as energy fuel and signaling molecules. The RNA-binding proteins RBM24 and RBM38 share an identical RNA-binding domain and thereby, regulate a group of same targets, such as p21. However, it is not certain whether RBM24 and RBM38 participates in lipid homeostasis. Here, lipidomic analysis showed that a deficiency in RBM24 or RBM38 leads to altered lipid metabolism, with more profound alteration by loss of RBM24 in MCF7 cells. We also showed that mice deficient in RBM24 were prone to chronic inflammation and liver steatosis, but not spontaneous tumors. These data let us speculate whether RBM24 regulates ferroptosis, a programmed cell death that links inflammation and liver steatosis via lipid peroxidation. Indeed, we found that over-expression of RBM24 protected, whereas knockout of RBM24 sensitized, cells to Erastin-induced ferroptosis by modulating the mRNA stability of SLC7A11, a ferroptosis inhibitor. Moreover, we showed that knockdown of SLC7A11 reversed the effect of RBM24 on ferroptosis. Together, our study revealed that RBM24 regulates lipid metabolism and SLC7A11 mRNA stability to modulate ferroptosis and inflammatory response.
Editorial: Advances in genomic and genetic tools, and their applications for understanding embryonic development and human diseases
Authors: Abu-Elmagd Muhammad , Assidi Mourad , Alrefaei Abdulmajeed F. , Rebai Ahmed
Published: November 2022

Significant advances have been recently made in the development of the genetic and genomic platforms. This has greatly contributed to a better understanding of gene expression and regulation machinery. Consequently, this led to considerable progress in unraveling evidence of the genotype-phenotype correlation between normal/abnormal embryonic development and human disease complexity. For example, advanced genomic tools such as next-generation sequencing, and microarray-based CGH have substantially helped in the identification of gene and copy number variants associated with diseases as well as in the discovery of causal gene mutations. In addition, bioinformatic analysis tools of genome annotation and comparison have greatly aided in data analysis for the interpretation of the genetic variants at the individual level. This has unlocked potential possibilities for real advances toward new therapies in personalized medicine for the targeted treatment of human diseases. However, each of these genomic and bioinformatics tools has its limitations and hence further efforts are required to implement novel approaches to overcome these limitations. It could be possible that the use of more than one platform for genotype-phenotype deep analysis is an effective approach to disentangling the cause and treatment of the disease complexities. Our research topic aimed at deciphering these complexities by shedding some light on the recent applications of the basic and advanced genetic/genomic and bioinformatics approaches. These include studying gene-gene, protein-protein, and gene-environment interactions. We, in addition, aimed at a better understanding of the link between normal/abnormal embryonic development and the cause of human disease induction.
Unfolding the role of placental-derived Extracellular Vesicles in Pregnancy: From homeostasis to pathophysiology
Authors: Ortega Miguel A. , Fraile-Martínez Oscar , García-Montero Cielo , Paradela Alberto , Asunción Sánchez-Gil María , Rodriguez-Martin Sonia , De León-Luis Juan A. , Pereda-Cerquella Claude , Bujan Julia , Guijarro Luis G. , Alvarez-Mon Melchor , García-Honduvilla Natalio
Published: November 2022

The human placenta is a critical structure with multiple roles in pregnancy, including fetal nutrition and support, immunological, mechanical and chemical barrier as well as an endocrine activity. Besides, a growing body of evidence highlight the relevance of this organ on the maternofetal wellbeing not only during gestation, but also from birth onwards. Extracellular vesicles (EVs) are complex macromolecular structures of different size and content, acting as carriers of a diverse set of molecules and information from donor to recipient cells. Since its early development, the production and function of placental-derived EVs are essential to ensure an adequate progress of pregnancy. In turn, the fetus receives and produce their own EVs, highlighting the importance of these components in the maternofetal communication. Moreover, several studies have shown the clinical relevance of EVs in different obstetric pathologies such as preeclampsia, infectious diseases or gestational diabetes, among others, suggesting that they could be used as pathophysiological biomarkers of these diseases. Overall, the aim of this article is to present an updated review of the published basic and translational knowledge focusing on the role of placental-derived EVs in normal and pathological pregnancies. We suggest as well future lines of research to take in this novel and promising field.
Tsukushi proteoglycan maintains RNA splicing and developmental signaling network in GFAP-expressing subventricular zone neural stem/progenitor cells
Authors: Istiaq Arif , Umemoto Terumasa , Ito Naofumi , Suda Toshio , Shimamura Kenji , Ohta Kunimasa
Published: November 2022

Tsukushi (TSK) proteoglycan dysfunction leads to hydrocephalus, a condition defined by excessive fluid collection in the ventricles and lateral ventricular enlargement. TSK injections into the LV at birth are effective at rescuing the lateral ventricle (LV). TSK regulates the activation of the Wnt signaling to facilitate the proper expansion of the LV and maintain the fate of the neural stem cell lineage. However, the molecular mechanism by which TSK acts on neural stem/progenitor cells (NSCs) during LV development is unknown. We demonstrated that TSK is crucial for the splicing and development-associated gene regulation of GFAP-expressing subventricular zone (SVZ) NSCs. We isolated GFAP-expressing NSCs from the SVZ of wild-type (GFAPGFP/+/TSK+/+) and TSK knock-out (GFAPGFP/+/TSK−/−) mice on postnatal day 3 and compared their transcriptome and splicing profiles. TSK deficiency in NSCs resulted in genome-wide missplicing (alteration in exon usage) and transcriptional dysregulation affecting the post-transcriptional regulatory processes (including splicing, cell cycle, and circadian rhythm) and developmental signaling networks specific to the cell (including Wnt, Sonic Hedgehog, and mTOR signaling). Furthermore, TSK deficiency prominently affected the splicing of genes encoding RNA and DNA binding proteins in the nervous SVZ and non-nervous muscle tissues. These results suggested that TSK is involved in the maintenance of correct splicing and gene regulation in GFAP-expressing NSCs, thereby protecting cell fate and LV development. Hence, our study provides a critical insight on hydrocephalus development.
Caulerpa racemosa extract inhibits HeLa cancer cells migration by altering expression of epithelial-mesenchymal transition proteins
Authors: Permatasari Happy Kurnia , Barbara Ulfa Ektina Naura , Adyana Daud Vanessa Pradna , Sulistomo Hikmawan Wahyu , Nurkolis Fahrul
Published: November 2022

Introduction: Cervical cancer is caused by persistent infections of human papillomavirus types 16 and 18. Also, it is classified as a malignancy since it is able to spread itself to other sites and form a metastasis. Lymph nodes metastasis is an important factor related to cervical cancer survival. The previous study reported that Caulerpa racemosa has an anti-cancer effect by inducing apoptosis by inhibiting p53 protein degradation in HeLa cancer cells. In this study, we conducted a follow-up test to determine the anticancer effect of Caulerpa racemosa as an antimetastatic agent on HeLa cancer cells.

Methods: A true experimental study with a post-test-controlled group design was carried out on four groups of HeLa cell cultures by presenting different concentrations of Caulerpa racemosa extract. Moreover, to identify the antimetastatic effect, HeLa cells treated with Caulerpa racemosa extract were subjected to the woud healing scratch test and immunofluorescence staining assays. Data analysis was gained with qualitative and quantitative approaches. Quantitative methods such as One-way analysis of variance, Tukey’s multiple comparison test, and Pearson’s correlation were conducted.

Result: We found that Caulerpa racemosa significantly inhibit HeLa cells wound healing migration. We also demonstrated the effect of Caulerpa racemosa in downregulating Snail and Vimentin protein expression and upregulating E-Cadherin protein expression.

Conclusion:Caulerpa racemosa extract inhibits HeLa cancer cells migration by altering important regulator proteins expressions of epithelial-mesenchymal transition pathways.
Progress of photoacoustic imaging combined with targeted photoacoustic contrast agents in tumor molecular imaging
Authors: Zheng Yiwen , Liu Mengyao , Jiang Lixin
Published: November 2022

Molecular imaging visualizes, characterizes, and measures biological processes at the molecular and cellular level. In oncology, molecular imaging is an important technology to guide integrated and precise diagnosis and treatment. Photoacoustic imaging is mainly divided into three categories: photoacoustic microscopy, photoacoustic tomography and photoacoustic endoscopy. Different from traditional imaging technology, which uses the physical properties of tissues to detect and identify diseases, photoacoustic imaging uses the photoacoustic effect to obtain the internal information of tissues. During imaging, lasers excite either endogenous or exogenous photoacoustic contrast agents, which then send out ultrasonic waves. Currently, photoacoustic imaging in conjunction with targeted photoacoustic contrast agents is frequently employed in the research of tumor molecular imaging. In this study, we will examine the latest advancements in photoacoustic imaging technology and targeted photoacoustic contrast agents, as well as the developments in tumor molecular imaging research.
Enhanced protein–protein interaction network construction promoted by in vivo cross-linking with acid-cleavable click-chemistry enrichment
Authors: Zhao Lili , Zhong Bowen , An Yuxin , Zhang Weijie , Gao Hang , Zhang Xiaodan , Liang Zhen , Zhang Yukui , Zhao Qun , Zhang Lihua
Published: November 2022

Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enables global profiling of protein interactome with direct interaction interfaces in complex biological systems. The alkyne-tagged enrichable cross-linkers are preferred to improve the coverage of low-abundance cross-linked peptides, combined with click chemistry for biotin conjugation to allow the cross-linked peptide enrichment. However, a systematic evaluation on the efficiency of click approaches (protein-based or peptide-based) and diverse cleavable click-chemistry ligands (acid, reduction, and photo) for cross-linked peptide enrichment and release is lacking. Herein, together with in vivo chemical cross-linking by alkyne-tagged cross-linkers, we explored the click-chemistry-based enrichment approaches on protein and peptide levels with three cleavable click-chemistry ligands, respectively. By comparison, the approach of protein-based click-chemistry conjugation with acid-cleavable tags was demonstrated to permit the most cross-linked peptide identification. The advancement of this strategy enhanced the proteome-wide cross-linking analysis, constructing a 5,518-protein–protein-interaction network among 1,871 proteins with widely abundant distribution in cells. Therefore, all these results demonstrated the guideline value of our work for efficient cross-linked peptide enrichment, thus facilitating the in-depth profiling of protein interactome for functional analysis.
Alterations of the gut microbiota and short chain fatty acids in necrotizing enterocolitis and food protein-induced allergic protocolitis infants: A prospective cohort study
Authors: Xiong Jing , Liao Xing-Sheng , Yin Tong , Liu Xiao-Chen , Bao Lei , Li Lu-Quan
Published: November 2022

Background

Even though presenting with similar clinical manifestations, necrotizing enterocolitis (NEC) and food protein-induced allergic protocolitis (FPIAP) have completely different treatments and prognosis. Our study aimed to quantify and evaluate differences in gut microbiota and short chain fatty acids (SCFAs) between infants with NEC and FPIAP to better identify these two diseases in clinical settings.

Methods

A total of 43 infants with NEC or FPIAP in Children’s Hospital of Chongqing Medical University, China between December 2020 and December 2021 were enrolled. Stool samples were prospectively collected and froze. Infants defined as NEC were those who presented with clinical courses consistent with NEC and whose radiographs fulfilled criteria for Bell’s stage 2 or 3 NEC, while those who were healthy in appearance and had blood in the stool (visible or may be microscopic), had normal bowel sounds in physical examination, were resolved after eliminating the causative food, and/or had recurrence of symptoms after oral food challenge (OFC) were defined as FPIAP. Primers specific for bacterial 16S rRNA genes were used to amplify and pyrosequence fecal DNA from stool samples. Gas chromatography-mass spectrometry (GC-MS) technology was used to determine the concentrations of SCFAs.

Results

Among the 43 infants, 22 were diagnosed with NEC and 21 were diagnosed with FPIAP. The microbial community structure in NEC infant stools differed significantly from those in FPIAP infant stools. NEC infants had significantly higher proportion of Actinobacteria and reduced proportion of Bacteroidetes compared with FPIAP infants, and the proportions of Halomonas, Acinetobacter, Bifidobacterium, and Stenotrophomonas in NEC infants were significantly higher than that of FPIAP infants. In addition, infants with NEC had significantly lower levels of acetic acid, propionic acid, butyric acid, isovaleric acid, and total SCFAs, and higher level of hexanoic acid as compared to the infants of the FPIAP group.

Conclusions

The differences of gut microbiota composition and concentrations of SCFAs might represent suitable biomarker targets for early identification of NEC and FPIAP.
Fatty acid uptake in Trypanosoma brucei: Host resources and possible mechanisms
Authors: Poudyal Nava Raj , Paul Kimberly S.
Published: November 2022

Trypanosoma brucei spp. causes African Sleeping Sickness in humans and nagana, a wasting disease, in cattle. As T. brucei goes through its life cycle in its mammalian and insect vector hosts, it is exposed to distinct environments that differ in their nutrient resources. One such nutrient resource is fatty acids, which T. brucei uses to build complex lipids or as a potential carbon source for oxidative metabolism. Of note, fatty acids are the membrane anchoring moiety of the glycosylphosphatidylinositol (GPI)-anchors of the major surface proteins, Variant Surface Glycoprotein (VSG) and the Procyclins, which are implicated in parasite survival in the host. While T. brucei can synthesize fatty acids de novo, it also readily acquires fatty acids from its surroundings. The relative contribution of parasite-derived vs. host-derived fatty acids to T. brucei growth and survival is not known, nor have the molecular mechanisms of fatty acid uptake been defined. To facilitate experimental inquiry into these important aspects of T. brucei biology, we addressed two questions in this review: (1) What is known about the availability of fatty acids in different host tissues where T. brucei can live? (2) What is known about the molecular mechanisms mediating fatty acid uptake in T. brucei? Finally, based on existing biochemical and genomic data, we suggest a model for T. brucei fatty acid uptake that proposes two major routes of fatty acid uptake: diffusion across membranes followed by intracellular trapping, and endocytosis of host lipoproteins.
Prevalence and possible factors of cognitive frailty in the elderly with hypertension and diabetes
Authors: Lu Shourong , Xu Qiao , Yu Jie , Yang Ying , Wang Zhuo , Zhang Bingshan , Wang Shuqiang , Chen Xiaorong , Zhang Yunyun , Zhu Xiaowei , Hong Kan
Published: November 2022

Background

Cognitive frailty is the coexistence of physical frailty and mild cognitive impairment. Research shows that cognitive frailty is related to an increased risk of hospitalization, mortality, disability, and dementia. Diabetes and hypertension are common risk factors for physical frailty and cognitive impairment. However, the factors influencing cognitive frailty in the elderly with hypertension and diabetes are still unclear. This study aimed to investigate the possible factors influencing cognitive frailty in the elderly with hypertension and diabetes.

Methods

A cross-sectional study was conducted. We evaluated people over 60 years with hypertension and diabetes who underwent physical examination in Wuxi Xin'an Community Health Service Center. Frail scale, Montreal Cognitive Assessment-Basic and clinical dementia rating were used to assess cognitive frailty. We collected demographic characteristics, hypertension and diabetes-related laboratory indicators of the participants. We also used various scales to assess the overall health status of the elderly.

Results

Approximately 20.8% of the participants were determined to have cognitive frailty in elderly adults with hypertension and diabetes. These participants were older, had a lower monthly income, and included a higher proportion of peasants. They also had a higher level of depression (p = 0.037), higher risk of falls (p = 0.000), higher risk of malnutrition (p = 0.002), poorer ability to perform activities of daily living (ADL) (p = 0.000), and less social support (p = 0.030). Multivariate regression analysis was used to further assess the factors for cognitive frailty. After adjusting for possible confounders, age and ADL score emerged as risk factors, whereas high monthly income decreased the risk of cognitive frailty.

Conclusion

Cognitive frailty is correlated with age, income, and ability to perform daily living activities in the elderly with diabetes and hypertension. Closer attention to the elderly who have low income and poor self-care ability may play an important role in the early prevention of cognitive frailty and even dementia.
Prognostic value of a left atrioventricular coupling index in pre- and post-menopausal women from the Multi-Ethnic Study of Atherosclerosis
Authors: Pezel Théo , Michos Erin D. , Varadarajan Vinithra , Shabani Mahsima , Venkatesh Bharath Ambale , Vaidya Dhananjay , Kato Yoko , De Vasconcellos Henrique Doria , Heckbert Susan R. , Wu Colin O. , Post Wendy S. , Bluemke David A. , Allison Matthew A. , Henry Patrick , Lima Joao A. C.
Published: November 2022

Background

Sex hormones associated with both the left atrial (LA) and left ventricular (LV) structures in women, but the association of menopause status with left atrioventricular coupling is not established.

Aim

To assess the prognostic value of a left atrioventricular coupling index (LACI) in peri-menopausal women without a history of cardiovascular disease (CVD).

Materials and methods

In all women participating in MESA study with baseline cardiovascular MRI, the LACI was measured as the ratio of the LA end-diastolic volume to the LV end-diastolic volume. Cox models were used to assess the association between the LACI and the outcomes of atrial fibrillation (AF), heart failure (HF), coronary heart disease (CHD) death, and hard CVD.

Results

Among the 2,087 women participants (61 ± 10 years), 485 cardiovascular events occurred (mean follow-up: 13.2 ± 3.3 years). A higher LACI was independently associated with AF (HR 1.70; 95%CI [1.51–1.90]), HF (HR 1.62; [1.33–1.97]), CHD death (HR 1.36; [1.10–1.68]), and hard CVD (HR 1.30; [1.13–1.51], all p < 0.001). Adjusted models with the LACI showed significant improvement in model discrimination and reclassification when compared to traditional models to predict: incident AF (C-statistic: 0.82 vs. 0.79; NRI = 0.325; IDI = 0.036), HF (C-statistic: 0.84 vs. 0.81; NRI = 0.571; IDI = 0.023), CHD death (C-statistic: 0.87 vs. 0.85; NRI = 0.506; IDI = 0.012), hard CVD (C-statistic: 0.78 vs. 0.76; NRI = 0.229; IDI = 0.012). The prognostic value of the LACI had a better discrimination and reclassification than individual LA or LV parameters.

Conclusion

In a multi-ethnic population of pre- and post-menopausal women, the LACI is an independent predictor of HF, AF, CHD death, and hard CVD.

Clinical trial registration

[https://clinicaltrials.gov/], identifier [NCT00005487].
Variable element enrichment sources and contributions to volcanic rocks along the Lesser Antilles Island Arc
Authors: Atlas Zachary D. , Germa Aurélie , Boss Brenna , Meireles Osvaldo , Ward Ami , Ryan Jeffrey G.
Published: November 2022

Understanding relationships between different components involved in subduction-related recycling of sediment at arc volcanic systems has long been a focus of study. It is generally understood that volcanic arc lavas incorporate materials from the down-going slab including fluids, fluid mobile elements (FME), and fluid modified large ion lithophile elements (LILE), largely derived from subducted sediments and serpentinized ultramafic rocks. Past studies have presumed bulk sediment contributions rather than addressing localized variations in sediment composition. We examine trace element variability along the Lesser Antilles Island Arc (LAIA) and show that sediment inputs via fluid transfer to the mantle wedge are necessary to explain high B (up to 50 ppm), high B/La ratios, and higher As, Cs, Rb in the central LAIA. In contrast, the northern portion of LAIA is enriched in Ba, Sr and U/Th, with low FME. Grenada has low FME abundances, low Ba/La, but higher Sm/La. Our results suggest that modification of LAIA mantle source by fluid addition is important for the central islands but find that sediment enrichment is more likely in the northern section of the LAIA. Li/Yb ratios suggest the Grenada signature is chemically more comparable to sediment from DSDP Site 144 (Demerara Rise), while Martinique magmas in the central LAIA are affiliated to sediments from Site 543 (offshore Dominica). Unexpectedly, the northern portion of LAIA is not directly relatable to Site 543 sediments but instead trends back toward a sediment source similar in composition to Site 144 but with lower B, lower La/Sm and higher Ba. Binary mixing models suggest the northern islands are likely influenced by small amounts of sediment melt similar to average Site 144 sediments, but with Ba concentrations ranging from 1,250 to 1,500 ppm. Trace element ratios then likely result from different levels of contribution from sediment packages both spatially and temporally along the arc, with some variation evident among samples from the same island. We suggest that the process of recycling subducted sediment along the arc is variable along the length of the LAIA, wherein the trace element relationships are likely strongly influenced by the types of sediment being subducted along strike.
Education for what? Human capital, human rights, and protection discourses in the COVID-19 response
Authors: Boisvert Kayla
Published: November 2022

The rallying cry of global actors working on education during COVID-19 has been that the pandemic poses a grave threat to ensuring equitable and inclusive access to high quality education for all children and adolescents, grounding the response firmly in the domain of global commitments embodied in Sustainable Development Goal 4, as well as prior commitments to education for all. However, while the idea of a global goal signifies cohesion around a shared set of ideals and actions, the meaning of these global commitments is contested terrain. It is just this terrain—alongside other cultural, political, and economic forces—that has the potential to affect national education responses to COVID-19, as well as the meaning of education writ large. This article explores three discourses—Education for Human Capital, Education as a Human Right, and Education for Protection—during COVID-19, demonstrating the way the idea of a collective response to the pandemic masks deep ideological difference between global actors. The article traces the evolution of these discourses in education in development and education in emergencies, situating them within broader cultural, political, and economic phenomena. This history is used to ground an analysis of the discourses within the COVID-19 education response and raises questions about the impacts these discourses may have on national education systems and education writ large during and beyond the pandemic.
The association between metabolic parameters and evening chronotype and social jetlag in non-shift workers: A meta-analysis
Authors: Zhang Rui , Cai Xiaoling , Lin Chu , Yang Wenjia , Lv Fang , Wu Jing , Ji Linong
Published: November 2022

Aims

The aim of the study was to evaluate the association between evening chronotype and social jetlag (SJL) with obesity, blood glucose and lipid levels in non-shift working adults.

Methods

The databases of MEDLINE, EMBASE and Cochrane Reviews were searched for studies analyzing the metabolic parameters among groups of different chronotypes or SJL until Feb 2022. Weighted mean difference (WMD) and 95% confidence intervals (CI) were used to analyze the association between these parameters and chronotypes or SJL.

Results

A total of 27 studies were included in this meta-analysis. Compared with morning chronotype, the participants with evening chronotype had higher body mass index (BMI) (WMD= 0.44 kg/m2, 95%CI, 0.30 to 0.57 kg/m2, p<0.001), higher fasting blood glucose level (WMD= 5.83mg/dl, 95%CI, 3.27to 8.38 mg/dl, p<0.001), higher total cholesterol level (WMD= 6.63mg/dl, 95%CI, 0.69 to 12.56 mg/dl, p=0.03), and lower high density lipoprotein cholesterol (HDL-C) level (WMD= -1.80mg/dl, 95%CI, -2.30 to -1.31 mg/dl, p<0.001). Compared with the participants with small SJL, the participants with large SJL had larger waist circumference (WMD= 0.80cm, 95%CI, 0.77 to 0.83cm, p<0.001).

Conclusions

Evening chronotype and SJL were associated with obesity and unfavorable metabolic parameters of glucose and lipid metabolism.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022303401.
The effects of urbanization on air pollution based on a spatial perspective: Evidence from China
Authors: Zhang Li , You Shibing , Zhang Miao , Zhang Shiwen , Yi Shuaixiang , Zhou Baokang
Published: November 2022

The severity of haze pollution has increased along with the growth of the economy and urbanization. Studying the relationship between urbanization and environmental pollution is extremely important from a practical standpoint in the context of encouraging new urbanization development in China. In this paper, we explore the impact mechanism of urbanization on air pollution, using a spatial Durbin model (SDM) build on panel data of 277 cities from 2010 to 2019. The findings demonstrate that urbanization significantly inhibits haze pollution across the country, with energy consumption structure and industrial structure upgrading acting as mediating factors. Moreover, economic growth, population aggregation, and openness to foreign investment aggravate pollution, whereas transportation facilities, urban vegetation areas, and wind speed reduce air pollution. Furthermore, foreign direct investment increases pollution nationwide while alleviating haze pollution in the eastern region. According to analysis of regional heterogeneity, there is a nonlinear relationship between urbanization and pollution in the central and western cities, while development and pollution in the eastern cities show a tendency to decouple. On this basis, this paper puts forward some policy recommendations to attenuate the vicious linkage between urbanization and pollution in order to promote high-quality urbanization development.
Implications of sanitation for rural resident health: Evidence and mechanisms
Authors: Huang Li , Chen Shupeng , Wu Heng , He Yuhong , Zhou Mi
Published: November 2022

This paper uses field survey data from rural areas of Liaoning Province, China to investigate the relationship between sanitation and the health of rural residents. The results show that the use of traditional latrine pits is significantly negatively correlated with the health of rural residents. Robustness tests using Propensity Score Matching (PSM) further demonstrate the consistency of the results. Further research suggests that traditional latrine pits may affect the health of rural residents indirectly through the poor eating habits of rural residents and by polluting drinking water sources. Using the Life satisfaction approach method, this study concludes that the health value of flush toilets in the sample area accounts for 98% of annual per capita household income.
Spatial and temporal overlap of domestic cats (Felis catus) and native urban wildlife
Authors: Herrera Daniel J. , Cove Michael V. , McShea William J. , Decker Sam , Flockhart D. T. Tyler , Moore Sophie M. , Gallo Travis
Published: November 2022

Free-roaming domestic cats (Felis catus) are known to pose threats to ecosystem health via transmission of zoonotic diseases and predation of native wildlife. Likewise, free-roaming cats are also susceptible to predation or disease transmission from native wildlife. Physical interactions are required for many of these risks to be manifested, necessitating spatial and temporal overlap between cats and wildlife species. Therefore, knowledge of the location and extent of shared habitat and activity periods would benefit management programs. We used data from a 3-year camera trap survey to model species-specific occupancy and identify landscape variables that contribute to the distribution of free-roaming domestic cats and eight native mammal species in Washington, DC. (USA). Our analysis includes five species that are common prey items of domestic cats, and three species that are potential disease vectors or are otherwise known to be a risk to cats. We then predicted the probability of occupancy and estimated the probability of spatial overlap between cats and each native wildlife species at multiple scales. We also used kernel density estimations to calculate temporal overlap between cats and each native wildlife species. Across spatial scales, occupancy for potential disease vector species was generally positively correlated with canopy cover and open water. Prey species were also generally positively correlated with canopy cover, but displayed negative associations with human population density and inconsistent associations with average per capita income. Domestic cat occupancy was negatively correlated with natural habitat characteristics and positively correlated with human population density. Predicted spatial overlap between domestic cats and native wildlife was greatest for potential disease vector species. Temporal overlap was high (>0.50) between cats and all but two native wildlife species, indicating that temporal overlap is probable wherever species overlap spatially. Our findings indicate that the risk to and from domestic cats varies across urban landscapes, but primarily arises from human activities. As such, humans are implicated in the negative outcomes that result from cats interacting with wildlife. Data-driven management to reduce such interactions can aid in cat population management, biodiversity conservation, and public health campaigns.
Labor induction information leaflets—Do women receive evidence-based information about the benefits and harms of labor induction?
Authors: von Dadelszen Peter , Tohill Susan , Wade Julie , Hutcheon Jennifer A. , Scott Janet , Green Marcus , Thornton James G. , Magee Laura A. ,   the WILL Pilot Trial Study Group
Published: November 2022

Objectives

To determine the extent to which a sample of NHS labor induction leaflets reflects evidence on labor induction.

Setting

Audit of labor induction patient information leaflets—local from WILL trial (When to Induce Labor to Limit risk in pregnancy hypertension) internal pilot sites or national-level available online.

Methods

Descriptive analysis [n = 21 leaflets, 19 (one shared) in 20 WILL internal pilot sites and 2 NHS online] according to NHS “Protocol on the Production of Patient Information” criteria: general information (including indications), why and how induction is offered (including success and alternatives), and potential benefits and harms.

Results

All leaflets described an induction indication. Most leaflets (n = 18) mentioned induction location and 16 the potential for delays due to delivery suite workloads and competing clinical priorities. While 19 leaflets discussed membrane sweeping (17 as an induction alternative), only 4 leaflets mentioned balloon catheter as another mechanical method. Induction success (onset of active labor) was presented by a minority of leaflets (n = 7, 33%), as “frequent” or in the “majority”, with “rare” or “occasional” failures. Benefits, harms and outcomes following induction were not compared with expectant care, but rather with spontaneous labor, such as for pain (n = 14, with nine stating more pain with induction). Potential benefits of induction were seldom described [n = 7; including avoiding stillbirth (n = 4)], but deemed to be likely. No leaflet stated vaginal birth was more likely following induction, but most stated Cesarean was not increased (n = 12); one leaflet stated that Cesarean risks were increased following induction. Women's satisfaction was rarely presented (n = 2).

Conclusion

Information provided to pregnant women regarding labor induction could be improved to better reflect women's choice between induction and expectant care, and the evidence upon which treatment recommendations are based. A multiple stakeholder-involved and evidence-informed process to update guidance is required.
Integrative analysis indicates the prognostic value of circadian rhythm disruption in liver cancer: Potential for therapeutic targeting
Authors: Wang Rui-Qi , Cui Wei , Cai Jiayi , Sun Yihao
Published: November 2022

Circadian rhythms regulate various biological processes, such as cell division and metabolism. Circadian rhythm disruption (CRD) is often associated with malignant tumor progression and poor prognosis. However, the effect of CRD on liver cancer prognosis has not been systematically analyzed or fully elucidated. Here, we developed a method to quantify and assess intratumoral CRD in a single-cell transcriptomic analysis of liver cancer and systematically analyzed the role of CRD in tumor progression and prognosis. Furthermore, a LASSO-Cox regression model based on 14 CRD genes was used to predict overall patient survival across multiple datasets. We found that malignant cells with high CRD scores were enriched in specific metabolic pathways, such as fatty acid metabolism and the trichloroacetic acid cycle. Intercellular communication analysis suggested that CRD regulates chemokine-mediated interactions. With the bulk transcriptomic datasets, we determined that LiverCRD scores were significantly correlated with macrophage infiltration levels and could guide targeted immunotherapy and chemotherapy strategies. In addition, LiverCRD is also associated with the mutational landscape—for example, TP53 mutation frequency was higher in high-CRD samples. Finally, the 14-gene-based LASSO-Cox regression model could accurately predict overall patient survival across datasets. In conclusion, Our proposed analysis reflects the relationship between CRD and the immune environment in liver cancer, suggesting that CRD may serve as a potential prognostic indicator. Our results may help guide targeted anti-tumor strategies.
Identification of a claudin-low subtype in clear cell renal cell carcinoma with implications for the evaluation of clinical outcomes and treatment efficacy
Authors: Zhang Cuijian , Li Yifan , Qian Jinqin , Zhu Zhenpeng , Huang Cong , He Zhisong , Zhou Liqun , Gong Yanqing
Published: November 2022

Background

In bladder and breast cancer, the claudin-low subtype is widely identified, revealing a distinct tumor microenvironment (TME) and immunological feature. Although we have previously identified individual claudin members as prognostic biomarkers in clear cell renal cell carcinoma (ccRCC), the existence of an intrinsic claudin-low subtype and its interplay with TME and clinical outcomes remains unclear.

Methods

Transcriptomic and clinical data from The Cancer Genome Atlas (TCGA)- kidney clear cell carcinoma (KIRC) cohort and E-MTAB-1980 were derived as the training and validation cohorts, respectively. In addition, GSE40435, GSE53757, International Cancer Genome Consortium (ICGC) datasets, and RNA-sequencing data from local ccRCC patients were utilized as validation cohorts for claudin clustering based on silhouette scores. Using weighted correlation network analysis (WGCNA) and multiple machine learning algorithms, including least absolute shrinkage and selection operator (LASSO), CoxBoost, and random forest, we constructed a claudin-TME related (CTR) risk signature. Furthermore, the CTR associated genomic characteristics, immunity, and treatment sensitivity were evaluated.

Results

A claudin-low phenotype was identified and associated with an inferior survival and distinct TME and cancer immunity characteristics. Based on its interaction with TME, a risk signature was developed with robust prognostic prediction accuracy. Moreover, we found its association with a claudin-low, stem-like phenotype and advanced clinicopathological features. Intriguingly, it was also effective in kidney chromophobe and renal papillary cell carcinoma. The high CTR group exhibited genomic characteristics similar to those of claudin-low phenotype, including increased chromosomal instability (such as deletions at 9p) and risk genomic alterations (especially BAP1 and SETD2). In addition, a higher abundance of CD8 T cells and overexpression of immune checkpoints, such as LAG3, CTLA4 and PDCD1, were identified in the high CTR group. Notably, ccRCC patients with high CTR were potentially more sensitive to immune checkpoint inhibitors; their counterparts could have more clinical benefits when treated with antiangiogenic drugs, mTOR, or HIF inhibitors.

Conclusion

We comprehensively evaluated the expression features of claudin genes and identified a claudin-low phenotype in ccRCC. In addition, its related signature could robustly predict the prognosis and provide guide for personalizing management strategies.
Integrated analysis of multiple microarray studies to establish differential diagnostic models of Crohn’s disease and ulcerative colitis based on a metalloproteinase-associated module
Authors: Deng Jiang , Zhao Ning , Lv Li-ping , Ma Ping , Zhang Yang-yang , Xu Jin-bo , Zhou Xi-peng , Chen Zi-an , Zhang Yan-yu
Published: November 2022

Background

The ulcerative colitis (UC) and Crohn’s disease (CD) subtypes of inflammatory bowel disease (IBD) are autoimmune diseases influenced by multiple complex factors. The clinical treatment strategies for UC and CD often differ, indicating the importance of improving their discrimination.

Methods

Two methods, robust rank aggregation (RRA) analysis and merging and intersection, were applied to integrate data from multiple IBD cohorts, and the identified differentially expressed genes (DEGs) were used to establish a protein−protein interaction (PPI) network. Molecular complex detection (MCODE) was used to identify important gene sets. Two differential diagnostic models to distinguish CD and UC were establishedviaa least absolute shrinkage and selection operator (LASSO) logistic regression, and model evaluation was performed in both the training and testing groups, including receiver operating characteristic (ROC) curves, calibration plots and decision curve analysis (DCA). The potential value of MMP-associated genes was further verified using different IBD cohorts and clinical samples.

Results

Four datasets (GSE75214, GSE10616, GSE36807, and GSE9686) were included in the analysis. Both data integration methods indicated that the activation of the MMP-associated module was significantly elevated in UC. Two LASSO models based on continuous variable (Model_1) and binary variable (Model_2) MMP-associated genes were established to discriminate CD and UC. The results showed that Model_1 exhibited good discrimination in the training and testing groups. The calibration analysis and DCA showed that Model_1 exhibited good performance in the training group but failed in the testing group. Model_2 exhibited good discrimination, calibration and DCA results in the training and testing groups and exhibited greater diagnostic value. The effects of Model_1 and Model_2 were further verified in a new IBD cohort of GSE179285. The MMP genes exhibited high value as biomarkers for the discrimination of IBD patients using published cohort and immunohistochemistry (IHC) staining data. The MMP-associated gene levels were statistically significantly positively correlated with the levels of the differentially expressed cell types, indicating their potential value in differential diagnosis. The single-cell analysis confirmed that the expression of ANXA1 in UC was higher than that in CD.

Conclusion

MMP-associated modules are the main differential gene sets between CD and UC. The established Model_2 overcomes batch differences and has good clinical applicability. Subsequent in-depth research investigating how MMPs are involved in the development of different IBD subtypes is necessary.
Human gut microbiota-reactive DP8α regulatory T cells, signature and related emerging functions
Authors: Jotereau Francine , Alameddine Joudy , Teusan Raluca , Pédron Annabelle , Jouand Nicolas , Altare Frédéric , Godefroy Emmanuelle
Published: November 2022

In mice, microbiota-induced Tregs both maintain intestinal homeostasis and provide resistance to immuno-pathologies in the adult. Identifying their human functional counterpart therefore represents an important goal. We discovered, in the human colonic lamina propria and blood, a FoxP3-negative IL-10-secreting Treg subset, which co-expresses CD4 and CD8α (hence named DP8α) and displays a TCR-reactivity against Faecalibacterium prausnitzii, indicating a role for this symbiotic bacterium in their induction. Moreover, supporting their role in intestinal homeostasis, we previously reported both their drastic decrease in IBD patients and their protective role in vivo against intestinal inflammation, in mice. Here, we aimed at identifying the genomic, phenotypic and functional signatures of these microbiota-induced Tregs, towards delineating their physiological role(s) and clinical potential. Human F. prausnitzii-reactive DP8α Treg clones were derived from both the colonic lamina propria and blood. RNA-sequencing, flow cytometry and functional assays were performed to characterize their response upon activation and compare them to donor- and tissue-matched FoxP3+ Treg clones. DP8α Tregs exhibited a unique mixed Tr1-like/cytotoxic CD4+ T cell-profile and shared the RORγt and MAF master genes with mouse gut microbiota-induced FoxP3+ Tregs. We revealed their potent cytotoxic, chemotactic and IgA-promoting abilities, which were confirmed using in vitro assays. Therefore, besides their induction by a Clostridium bacterium, DP8α Tregs also partake master genes with mouse microbiota-induced Tregs. The present identification of their complete signature and novel functional properties, should be key in delineating the in vivo roles and therapeutic applications of these unique human microbiota-induced Tregs through their study in pathological contexts, particularly in inflammatory bowel diseases.