bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Lentinan on the Hematological and Immune Indices of Dairy Cows
Authors: Tan Lun , Fan Caiyun , Wang Dian , Li Xiao , Wang Meng , Zhuo Zhao , Li Shuaihong , Ding Yuhang , Yang Zixi , Cheng Jianbo
Published: April 2024

In this study, we investigated the effects of lentinan (LNT) on hematological parameters, immune indices, and metabolite levels in dairy cows. We randomly assigned forty Holstein cows to four treatment groups. The treatments consisted of 0, 5, 10, and 15 g/d of LNT. Compared with the control group, the addition of 10 g/d of LNT decreased the content of ALT and IL-8 but simultaneously increased the content of IL-4 in the cows’ serum. Supplementation with 10 g/d of LNT decreased the levels of lymphocyte, RDW, ALT, AST, TC, IL-2, and IL-8, but, concurrently, in-creased the levels of granulocytes and IL-4 in their serum. In addition, supplementation with 15 g/d of LNT decreased the levels of RDW, TC, IL-2, and IL-8, but, at the same time, increased the levels of IL-4 and IgM in their serum. For the metabolomic analysis, cows fed with 0 and 10 g/d of LNT were selected. The results showed that 10 metabolites, including reduced nicotinamide riboside and trehalose, were upregulated in the 10 g/d group. These differential metabolites were enriched in tyrosine metabolism and trehalose degradation and altered two metabolic pathways of ubiquinone and other terpene quinone biosynthesis, as well as starch and sucrose metabolism. These findings provide evidence that LNT could be used to reduce the risk of inflammation in dairy cows.
Age-Related Changes in Survival Behaviour in Parasite-Free Hatchery-Reared Rainbow Trout (Oncorhynchus mykiss)
Authors: Freire Rafael , Michie Mathea , Rogers Leia , Shamsi Shokoofeh
Published: April 2024

Millions of hatchery-reared Rainbow trout are currently released in Australian waters to support recreational fisheries objectives, yet many of these fish die soon after release. In addition, little is known whether these fish harbour parasites that can potentially threaten freshwater ecosystems and human health. Here, we tested the behaviour of hatchery-reared trout using six tank-based tests at six different ages to evaluate their chances of survival and then dissected fish to investigate parasite prevalence. At 7 weeks of age fish readily emerged from a hide and showed the greatest number of startle responses to predators. Behaviour around 25–29 weeks of age was relatively “shy”, staying in shelter and avoiding open water. At around 37–41 weeks of age though, behaviour changed, with fish emerging from a hide more readily and exploring the environment. Interestingly, at 58 weeks of age fish were slower to initiate exploration, possibly indicating a return to “shyer” behaviour. All fish underwent thorough parasite examination, revealing no infections. We conclude that knowledge of the behaviour of hatchery-reared fish at different ages is useful for decisions around the timing of release that balance the needs of recreational fishers whilst managing the impact on freshwater ecosystem.
Osteolytic Lesions in a Sub-Adult Loggerhead Sea Turtle (Caretta caretta): A Case Report
Authors: Peña Pascucci Ignacio , Pernas Mozas Susana , Garrido Sánchez Lucía
Published: April 2024

Osteolytic lesions in loggerhead sea turtles (Caretta caretta) during rehabilitation are attributed to multiple causes, including gas embolism, hypothermia, and osteomyelitis due to bacterial or fungal infection. This study reports the appearance of osteolytic lesions in a sub-adult loggerhead sea turtle with involvement of the right fore and hind flippers, visible swelling of the elbow and knee joints, and accompanied by lameness after 45 days of rehabilitation. Radiographs and computed tomography revealed multiple lytic bone lesions. This was the fourth rehabilitation admission of the turtle after being accidentally captured by trawler ships (bycatch) in 2019, 2020, 2022, and 2023. Potential causes were dysbaric osteonecrosis due to a past decompression sickness event and hypothermia with osteomyelitis from bacterial infection. Blood cultures and antibiotic susceptibility testing led to the isolation of Ewingella americana responsive to enrofloxacin. This study investigates extensive fore and hind flipper involvement in a sub-adult loggerhead turtle, aiming to determine causes and risk factors. The pathogenesis and significance of these lesions is discussed.
Effects of Two Different Methods of Teeth Grinding on Dental Injuries, Skin Lesions, Growth and Behaviour of Suckling Piglets Compared to a Non-Treated Control Group
Authors: Timphaus Carolin Bernarda , kleine Kruthaup Franziska Anna , Freise Fritjof , Herbrandt Swetlana , große Beilage Elisabeth , Fels Michaela
Published: April 2024

Teeth grinding in suckling piglets is performed on many farms to protect the piglets’ littermates and the sow’s udder from injuries caused by the piglets’ canines and third incisors. In this study, the effects of two teeth-grinding methods on the piglets’ dental health and welfare were investigated. The piglets of a litter were evenly assigned to a treatment: one-third of littermates were ground with a roller grinding head (RG), one-third with a teacup grinding head (TCG), and one-third were not ground at all (CG). A random sample of 100 animals each from the RG and TCG treatment was examined for tooth injuries, i.e., dental pulp openings. Additionally, behavioural analysis was performed (n = 650 piglets), and skin lesions, growth and mortality were determined (n = 1565 piglets). TCG piglets had a lower risk (p < 0.001) of pulp opening than RG piglets (0.08 ± 0.31 vs. 2.67 ± 1.67 opened pulps per piglet). Mortality, growth, skin lesions and behaviour of piglets were not influenced by treatment (p > 0.05). This study showed that both teeth-grinding methods led to pulp openings. If teeth grinding cannot be avoided on a farm, using the teacup grinding head is recommended concerning animal health and welfare.
Maternal Effects and Trophodynamics Drive Interannual Larval Growth Variability of Atlantic Bluefin Tuna (Thunnus thynnus) from the Gulf of Mexico
Authors: Quintanilla José M. , Borrego-Santos Ricardo , Malca Estrella , Swalethorp Rasmus , Landry Michael R. , Gerard Trika , Lamkin John , García Alberto , Laiz-Carrión Raúl
Published: April 2024

Two cohorts of Atlantic bluefin tuna (Thunnus thynnus) larvae were sampled in 2017 and 2018 during the peak of spawning in the Gulf of Mexico (GOM). We examined environmental variables, daily growth, otolith biometry and stable isotopes and found that the GOM18 cohort grew at faster rates, with larger and wider otoliths. Inter and intra-population analyses (deficient vs. optimal growth groups) were carried out for pre- and post-flexion developmental stages to determine maternal and trophodynamic influences on larval growth variability based on larval isotopic signatures, trophic niche sizes and their overlaps. For the pre-flexion stages in both years, the optimal growth groups had significantly lower δ15N, implying a direct relationship between growth potential and maternal inheritance. Optimal growth groups and stages for both years showed lower C:N ratios, reflecting a greater energy investment in growth. The results of this study illustrate the interannual transgenerational trophic plasticity of a spawning stock and its linkages to growth potential of their offsprings in the GOM.
Molecular Prevalence, Genetic Diversity, and Tissue Tropism of Bartonella Species in Small Mammals from Yunnan Province, China
Authors: Han Pei-Yu , Xu Fen-Hui , Tian Jia-Wei , Zhao Jun-Ying , Yang Ze , Kong Wei , Wang Bo , Guo Li-Jun , Zhang Yun-Zhi
Published: April 2024

Bartonella is an intracellular parasitic zoonotic pathogen that can infect animals and cause a variety of human diseases. This study investigates Bartonella prevalence in small mammals in Yunnan Province, China, focusing on tissue tropism. A total of 333 small mammals were sampled from thirteen species, three orders, four families, and four genera in Heqing and Gongshan Counties. Conventional PCR and real-time quantitative PCR (qPCR) were utilized for detection and quantification, followed by bioinformatic analysis of obtained DNA sequences. Results show a 31.5% detection rate, varying across species. Notably, Apodemus chevrieri, Eothenomys eleusis, Niviventer fulvescens, Rattus tanezumi, Episoriculus leucops, Anourosorex squamipes, and Ochotona Thibetana exhibited infection rates of 44.4%, 27.7%, 100.0%, 6.3%, 60.0%, 23.5%, and 22.2%, respectively. Genetic analysis identified thirty, ten, and five strains based on ssrA, rpoB, and gltA genes, with nucleotide identities ranging from 92.1% to 100.0%. Bartonella strains were assigned to B. grahamii, B. rochalimae, B. sendai, B. koshimizu, B. phoceensis, B. taylorii, and a new species identified in Episoriculus leucops (GS136). Analysis of the different tissues naturally infected by Bartonella species revealed varied copy numbers across different tissues, with the highest load in spleen tissue. These findings underscore Bartonella’s diverse species and host range in Yunnan Province, highlighting the presence of extensive tissue tropism in Bartonella species naturally infecting small mammalian tissues.
Isobutyramide and Slow-Release Urea as Substitutes for Soybean Meal in the Finishing Diet of Beef Cattle
Authors: Wei Chen , Tao Haiying , Liu Guifen , Tian Kechuan
Published: April 2024

Two experiments were conducted to investigate the effects of isobutyramide (IBA) and slow-release urea (SRU) as substitutes for soybean meal (SBM) in the finishing diet of beef cattle. The completely randomized design in vitro experiment with five treatments, i.e., control, 0.9% SRU group, 0.6% SRU + 0.3% IBA group (SRU-I), 0.3% SRU + 0.6% IBA group (IBA-S), 0.9% IBA group was conducted. The results showed that the IBA-S and IBA increased (p ≤ 0.05) substrate disappearance of dry matter (DM), neutral detergent fiber (NDF), acid detergent fiber (ADF), total gas, and total volatile fatty acids (TVFA). The SRU group had the highest (p < 0.01) crude protein disappearance and ammonia nitrogen concentration, but the IBA contrarily decreased (p < 0.01) them compared with the control. Inclusion of IBA increased isobutyrate concentrations (p = 0.01) with the highest value for the IBA group. Then, an 84-day replicate 4 × 4 Latin square design with 8 Angus steers and four treatments, i.e., control, SRU, SRU-I, IBA-S was performed. The results showed that the treatments did not affect DM intake (p > 0.05) but tended (p = 0.09) to increase average daily gain. The inclusion of IBA increased (p < 0.05) the apparent digestibility of DM, organic matter, NDF, ADF, TVFA, and microbial crude protein with the highest values for the IBA-S group. The IBA-contained groups also increased (p ≤ 0.01) isobutyrate concentration, activities of carboxymethyl cellulase and xylanase, and the relative abundance of Butyrivibrio fibrisolvens with the highest values for the IBA-S group. The SRU had no effect on animal growth and nutrient apparent digestibility. In conclusion, IBA was developed as a new substitute for SBM in the finishing diet of beef cattle, and the optimal strategy was the isonitrogenous substitution of SBM with 0.3% SRU and 0.6% IBA of the diet.
Effect of Nucleic Acid Analog Administration on Fluctuations in the Albumin-to-Globulin Ratio in Cats with Feline Infectious Peritonitis
Authors: Katayama Masato , Uemura Yukina , Katori Daichi
Published: April 2024

Background: feline infectious peritonitis (FIP) is a fatal disease in cats classified as either effusive (‘wet’), non-effusive (‘dry’), or a mixture of both forms (‘mixed’). The anti-FIP therapeutic effects of Mutian and molnupiravir, two drugs with a nucleic acid analog as an active ingredient, have been confirmed recently. Methods: Of the cats with FIP, we observed a total of 122 and 56 cases that achieved remission after the administration of Mutian and molnupiravir as routine treatments, respectively. Changes in clinical indicators suggested to be correlated with FIP remission (weight, hematocrit, and albumin-to-globulin ratio) before and after the administration of each drug and during follow-up observation were statistically compared for each FIP type. Results: In all three FIP types, the administration of either Mutian or molnupiravir resulted in statistically significant increases in these indicators. Furthermore, the effect of Mutian on improving the albumin-to-globulin ratio was not observed at all in wet FIP, as compared with that of molnupiravir, but statistically significant in mixed and dry (p < 0.02 and p < 0.003, respectively). The differences in albumin-to-globulin ratio were all due to those of circulating globulin levels. Conclusions: These results indicate that slight inflammatory responses might be elicited continuously by a residual virus that persisted through molnupiravir treatments.
POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes
Authors: Zhang Geyang , Wang Jiamei , Li Yulong , Zhang Zijing , Wang Xiangnan , Chen Fuying , Shi Qiaoting , Huang Yongzhen , Wang Eryao , Lyu Shijie
Published: April 2024

DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation. In this study, the muscle development-related gene POLB was screened by selection signature and RNA-seq analysis and then validated for the proliferation and apoptosis of bovine primary myocytes. It was also found that overexpression of the POLB gene had a pro-apoptosis effect, but interfering with the expression of the gene had no significant effect on cells. Then, the analysis of related apoptotic genes revealed that POLB overexpression affected CASP9 gene expression.
Analysis and Comparison of New-Born Calf Standing and Lying Time Based on Deep Learning
Authors: Zhang Wenju , Wang Yaowu , Guo Leifeng , Falzon Greg , Kwan Paul , Jin Zhongming , Li Yongfeng , Wang Wensheng
Published: April 2024

Standing and lying are the fundamental behaviours of quadrupedal animals, and the ratio of their durations is a significant indicator of calf health. In this study, we proposed a computer vision method for non-invasively monitoring of calves’ behaviours. Cameras were deployed at four viewpoints to monitor six calves on six consecutive days. YOLOv8n was trained to detect standing and lying calves. Daily behavioural budget was then summarised and analysed based on automatic inference on untrained data. The results show a mean average precision of 0.995 and an average inference speed of 333 frames per second. The maximum error in the estimated daily standing and lying time for a total of 8 calf-days is less than 14 min. Calves with diarrhoea had about 2 h more daily lying time (p < 0.002), 2.65 more daily lying bouts (p < 0.049), and 4.3 min less daily lying bout duration (p = 0.5) compared to healthy calves. The proposed method can help in understanding calves’ health status based on automatically measured standing and lying time, thereby improving their welfare and management on the farm.
Allergic Dermatitis in Pêga Breed Donkeys (Equus asinus) Caused by Culicoides Bites in the Amazon Biome, Pará, Brazil
Authors: Barbosa José Diomedes , Sodré Maria Hilma Soares , Barbosa Camila Cordeiro , da Costa Paulo Sérgio Chagas , Oliveira Carlos Magno Chaves , Ferreira Tatiane Teles Albernaz , da Silveira José Alcides Sarmento , Lamego Eryca Ceolin , Paz Milena Carolina , Caldeira Rossela Damasceno , Magalhães Matos Paulo César , Serruya Analiel , Salvarani Felipe Masiero , Silveira Natália da Silva e Silva
Published: April 2024

An allergy to bites from Culicoides (Diptera: Ceratopogonidae) occurs because of a hypersensitivity reaction caused by the inoculation of insect salivary antigens during the bite, resulting in immune-mediated dermatitis. To the best of our knowledge, no previous studies have focused on allergic dermatitis in donkeys in Brazil. Therefore, this study aimed to describe the epidemiological, clinicopathological, and therapeutic aspects of allergic dermatitis in donkeys and to identify the insects involved in its epidemiology. This study reported the occurrence of dermatitis in 17 animals. The clinical signs were restlessness and severe itching. Skin lesions were found on the head, depigmented areas of the muzzle and cheeks, flanks, pelvic and thoracic limbs, and the scrotal sac. The lesions were characterized by areas of alopecia with crusts accompanied by serosanguineous exudates. Histologically, the lesions were characterized as moderate superficial dermatitis with irregular epidermal acanthosis and pronounced diffuse orthokeratotic hyperkeratosis. In total, 378 Culicoides specimens were collected, with Culicoides ocumarensis Ortiz being the most abundant species. The combined application of copaiba oil and a multivitamin emulsion exhibited potential for topical treatment of allergic dermatitis caused by insect bites in donkeys. Our study revealed an association between allergic dermatitis in donkeys and Culicoides.
Analysis of the Immunogenicity of African Swine Fever F317L Protein and Screening of T Cell Epitopes
Authors: Huang Ying , Zhai Wenzhu , Wang Zhen , He Yuheng , Tao Chunhao , Chu Yuanyuan , Pang Zhongbao , Zhu Hongfei , Jia Hong
Published: April 2024

The African swine fever virus (ASFV) encodes numerous proteins characterized by complex immune escape mechanisms. At present, the structure and function of these proteins, including the F317L protein, have yet to be fully elucidated. In this study, we examined the immunogenicity of the F317L protein. Mice were subcutaneously immunized with the F317L protein using initial and subsequent booster doses, and, at the 28th day post-treatment, we assessed the humoral and cellular immune responses of mice. The F317L protein stimulated production of specific antibodies and activated humoral immune responses. In addition, F317L stimulated the production of large amounts of IFN-γ by splenic lymphocytes, thereby activating cellular immune responses. Using informatics technology, we predicted and synthesized 29 F317L protein T cell epitopes, which were screened using IFN-γ ELISpot. Among these, the F25 (246SRRSLVNPWT255) peptide was identified as having a stronger stimulatory effect than the full-length protein. Collectively, our findings revealed that the ASFV F317L protein can stimulate both strong humoral and cellular immunity in mice, and that the F25 (246SRRSLVNPWT255) peptide may be a potential active T cell epitope. These findings will provide a reference for further in-depth studies of the F317L protein and screening of antigenic epitopes.
Occurrence of Clostridium perfringens in Wild Mammals in the Amazon Biome
Authors: Oliveira Hanna Gabriela da Silva , Sousa Ananda Iara de Jesus , Zanon Isabela Paduá , Lopes Cinthia Távora de Albuquerque , Silva Rodrigo Otavio Silveira , Domingues Sheyla Farhayldes Souza , Salvarani Felipe Masiero
Published: April 2024

The objective of this study was to evaluate the occurrence of Clostridium perfringens in stool samples and swabs collected from wild mammals in the Amazon biome. Sixty-five faecal and swab samples were collected in situ and ex situ from 16 species and three genera of wild mammals, some of which were in good health and some of which had diarrhoea. After pre-enrichment, the samples were plated on selective agar for C. perfringens. Characteristic colonies were subjected to multiplex PCR for the detection of genes encoding the main C. perfringens toxins (alpha, beta, epsilon, and iota toxin and enterotoxin). Among the 65 samples, 40 (61.5%) were positive for the gene encoding the alpha toxin and were classified as type A, 36 of which were asymptomatic animals and four were diarrheal. No other toxinotypes were found. The findings of this study suggest that C. perfringens type A is commonly found in mammal species of the Amazon biome. This seems to be the first study to identify C. perfringens type A in species such as B. variegatus (common ground sloth), C. didactylus (two-toed sloth), P. flavus (Jupará), T. tetradactyla (anteater), S. collinsi (squirrel monkey), S. niger (black marmoset), and S. apella (Guyana capuchin) and in the genus Didelphis sp. (opossum).
Strand-Specific RNA Sequencing Reveals Gene Expression Patterns in F1 Chick Breast Muscle and Liver after Hatching
Authors: Zhao Jianfei , Chen Meiying , Luo Zhengwei , Cui Pengxin , Ren Peng , Wang Ye
Published: April 2024

Heterosis refers to the phenomenon where hybrids exhibit superior performance compared to the parental phenotypes and has been widely utilized in crossbreeding programs for animals and crops, yet the molecular mechanisms underlying this phenomenon remain enigmatic. A better understanding of the gene expression patterns in post-hatch chickens is very important for exploring the genetic basis underlying economically important traits in the crossbreeding of chickens. In this study, breast muscle and liver tissues (n = 36) from full-sib F1 birds and their parental pure lines were selected to identify gene expression patterns and differentially expressed genes (DEGs) at 28 days of age by strand-specific RNA sequencing (ssRNA-seq). This study indicates that additivity is the predominant gene expression pattern in the F1 chicken post-hatch breast muscle (80.6% genes with additivity) and liver (94.2% genes with additivity). In breast muscle, Gene Ontology (GO) enrichment analysis revealed that a total of 11 biological process (BP) terms closely associated with growth and development were annotated in the identified DEG sets and non-additive gene sets, including STAT5A, TGFB2, FGF1, IGF2, DMA, FGF16, FGF12, STAC3, GSK3A, and GRB2. Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation presented that a total of six growth- and development-related pathways were identified, involving key genes such as SLC27A4, GLUL, TGFB2, COX17, and GSK3A, including the PPAR signaling pathway, TGF-beta signaling pathway, and mTOR signaling pathway. Our results may provide a theoretical basis for crossbreeding in domestic animals.
Effect of Milk-Feeding Frequency and Calcium Gluconate Supplementation on Growth, Health, and Reproductive and Metabolic Features of Holstein Heifers at a Rearing Farm
Authors: Revilla-Ruiz Angel , Carulla Patricia , Fernandez-Novo Aitor , de Mercado Eduardo , Pérez-Navarro Alejandro , Patrón-Collantes Raquel , Sebastián Francisco , Pérez-Garnelo Sonia S. , González-Martín Juan V. , Estellés Fernando , Villagrá Arantxa , Astiz Susana
Published: April 2024

We compared the effects of milk-feeding in 288 Holstein calves (72 per group) which were fed twice (2F) or thrice (3F) daily, with or without the addition of hydrogenated fat-embedded calcium gluconate (G) supplemented in the starter food and in the daily diet up to the age of 9 months, on the calves’ metabolism, growth, health, and reproductive efficiency up to first pregnancy. The calves received 6 L of milk replacer (130 g/L) and had ad libitum access to water and textured calf starter with or without gluconate. Gluconate supplementation promoted a “catch-up” in growth in supplemented calves compared to their counterparts that did not receive gluconate. Gluconate appeared to reduce animal metabolic stress during key events, such as weaning and transfer into open-door pens, reducing fructosamine (352.61 vs. 303.06 in 3FG and 3F, respectively; p = 0.028) and urea (3F revealed the highest values compared with the other three groups: 19.06 for 3F vs. 13.9 (2F), 13.7 (2FG), and 14.3 (3FG), respectively, p = 0.002) from weaning onwards. The feeding of dairy calves with milk replacer three rather than two times per day tended to be associated with better health from weaning to 4 months old; parameters such as ultrasound lung score and calf health score improved over time (p < 0.001). Thrice-daily feeding with milk replacer tended to reduce the number of artificial inseminations per pregnancy in heifers by 0.2 points (p = 0.092). We confirmed significant correlations between early health and growth parameters and reproductive efficiency and a positive correlation between body weight and average daily weight gain and the thickness of the back fat layer in young heifers (r = 0.245; p < 0.0001; r = 0.214; p < 0.0001 respectively). Our study was conducted on a commercial farm with reasonably effective animal management, so baseline welfare was likely satisfactory.
Supplementation of Methionine Dipeptide Enhances the Milking Performance of Lactating Dairy Cows
Authors: Wei Xiaoshi , Han Ning , Liu Hongyun
Published: April 2024

Methionine dipeptide (Met-Met) could improve milk protein synthesis in bovine epithelia mammary cells and lactating mice, while the effects of Met-Met on lactation performance, rumen fermentation and microbiota profile in lactating dairy cows have not been explored. For this reason, 60 Chinese lactating Holstein cows were allocated into three treatment groups: control group (CON), 6 g/d methionine dipeptide group (MM), and 6.12 g/d rumen-protected methionine dipeptide group (RPMM). The experiment lasted for 10 weeks to monitor lactation performance, plasma amino acid profile and rumen fermentation parameters and microbiota profile. Results showed that MM increased the energy-corrected milk (ECM), and RPMM increased both milk yield and ECM (p < 0.05). The milk protein concentration and yield were increased by MM and RPMM (p < 0.05). The rumen fermentation showed that RPMM increased total volatile fatty acids, acetate and valerate concentrations (p < 0.05). The relative abundance of Firmicutes, including Succiniclasticum, Selenomonas and Clostridium_XlVa, were enriched and the Prevotella was decreased by RPMM (p < 0.05). In summary, daily supplementing with 6 g of MM or RPMM in lactating dairy cows could improve milk yield and both percentage and yield of milk protein, and RPMM benefited the rumen fermentation and altered the bacterial composition. These results provided the first evidence that Met-Met supplementation can improve lactation performance of dairy cows.
The Perspective of Metallic Access Networks Based on MELT Parameter Measurements on Homogeneous Symmetric Lines
Authors: Lichý Marek , Róka Rastislav
Published: April 2024

In this paper, attention is focused on the perspective of metallic access networks utilizing homogeneous symmetric lines as a transmission medium. This miscellaneous environment has a strong influence on transmitted Digital Subscriber Line (DSL) signals and hence it is necessary to know its specific parameters in the given case. Fast and precise measurements on homogeneous symmetric lines are still essential, meaningful and imperative for many reasons: solving interference problems, designing advanced signal processing techniques and realizing appropriate simulation models. There are various ways of testing measurements with meaningful differences. In this contribution, we are focusing on the importance of measurements on homogeneous symmetric lines by means of metallic ended line testing. Subsequently, possibilities for new evaluation techniques for Metallic Ended Line Testing (MELT) parameters and for analyzing of DSL service provision are presented. Additionally, the realization of a mobile application functionality for facilitating the jobs of technicians is introduced, together with the possible availability of homogeneous symmetric lines for DSL service provision.
Formation Control of Multiple Autonomous Mobile Robots Using Turkish Natural Language Processing
Authors: Aram Kadir , Erdemir Gokhan , Can Burhanettin
Published: April 2024

People use natural language to express their thoughts and wishes. As robots reside in various human environments, such as homes, offices, and hospitals, the need for human–robot communication is increasing. One of the best ways to achieve this communication is the use of natural languages. Natural language processing (NLP) is the most important approach enabling robots to understand natural languages and improve human–robot interaction. Also, due to this need, the amount of research on NLP has increased considerably in recent years. In this study, commands were given to a multiple-mobile-robot system using the Turkish natural language, and the robots were required to fulfill these orders. Turkish is classified as an agglutinative language. In agglutinative languages, words combine different morphemes, each carrying a specific meaning, to create complex words. Turkish exhibits this characteristic by adding various suffixes to a root or base form to convey grammatical relationships, tense, aspect, mood, and other semantic nuances. Since the Turkish language has an agglutinative structure, it is very difficult to decode its sentence structure in a way that robots can understand. Parsing of a given command, path planning, path tracking, and formation control were carried out. In the path-planning phase, the A* algorithm was used to find the optimal path, and a PID controller was used to follow the generated path with minimum error. A leader–follower approach was used to control multiple robots. A platoon formation was chosen as the multi-robot formation. The proposed method was validated on a known map containing obstacles, demonstrating the system’s ability to navigate the robots to the desired locations while maintaining the specified formation. This study used Turtlebot3 robots within the Gazebo simulation environment, providing a controlled and replicable setting for comprehensive experimentation. The results affirm the feasibility and effectiveness of employing NLP techniques for the formation control of multiple mobile robots, offering a robust and effective method for further research and development on human–robot interaction.
Design and Evaluation of Novel Submerged Floating Tunnel Models Based on Dynamic Similarity
Authors: Ren Hongyu , Guo Tong , Liu Zhongxiang , Zhi Guoliang , Xu Xiangyang
Published: April 2024

Submerged floating tunnels (SFTs), also known as the Archimedes Bridge, are new transportation structures designed for crossing deep waters. Compared with cross-sea bridges and subsea tunnels, SFTs offer superior environmental adaptability, reduced construction costs, and an enhanced spanning capacity, highlighting their significant development potential and research value. This paper introduces a new type of SFT scale model for hydrodynamic experiments, adhering to the criteria for geometric similarity, motion similarity, and dynamic similarity principles, including the Froude and Cauchy similarity principles. This model enables the accurate simulation of the elastic deformation of the tunnel body and complex hydrodynamic phenomena, such as fluid–structure interactions and vortex–induced vibrations. Moreover, this paper details the design methodology, fabrication process, and method for similarity evaluation, covering the mass, deflection under load, natural frequency in air, and the natural frequency of the various underwater motion freedoms of the model. The results of our experiments and numerical simulations demonstrate a close alignment, proving the reliability of the new SFT scale model. The frequency distribution observed in the white noise wave tests indicates that the SFT equipped with inclined mooring cables experiences a coupled interaction between horizontal motion, vertical motion, and rotation. Furthermore, the design methodology of this model can be applied to other types of SFTs, potentially advancing technical progress in scale modeling of SFTs and enhancing the depth of SFT research through hydrodynamic experiments.
Conditions When the Problems of Linear Programming Are Algorithmically Unsolvable
Authors: Chernov Viktor , Chernov Vladimir
Published: April 2024

We study the properties of the constructive linear programming problems. The parameters of linear functions in such problems are constructive real numbers. Solving such a problem involves finding the optimal plan with the constructive real number components. We show that it is impossible to have an algorithm that solves an arbitrary constructive real programming problem.
GHF-COPRAS Multiple Attribute Decision-Making Method Based on Cumulative Prospect Theory and Its Application to Enterprise Digital Asset Valuation
Authors: Liu Pingqing , Shen Junxin
Published: April 2024

With the rapid development of the economy, data have become a new production factor and strategic asset, enhancing efficiency and energy for technological innovation and industrial upgrading in enterprises. The evaluation of enterprise digital asset value (EDAV) is a typical multi-attribute decision-making (MADM) problem. Generalized hesitant fuzzy numbers (GHFNs) can better express the uncertainty and fuzziness of evaluation indexes, thus finding wide applications in MADM problems. In this paper, we first propose the Kullback–Leibler (K-L) divergence distance of GHFNs and prove its mathematical properties. Second, recognizing that decision-makers often have finite rationality in practical problems, we combine the cumulative prospect theory (CPT) with the Complex Proportional Assessment (COPRAS) method to propose the GHF-CPT-COPRAS model for solving MADM problems. Simultaneously, we extend the distance correlation-based Criteria Importance Through Intercriteria Correlation (D-CRITIC) method to the GHF environment to rationally calculate the weights of attributes in the EDAV evaluation problem. Finally, we apply the proposed GHF-CPT-COPRAS model to the EDAV evaluation problem and compare it with existing GHF decision-making methods to verify its effectiveness and feasibility. This study provides an important reference for addressing the EDAV assessment problem within an uncertain fuzzy environment and extends its application methods in the decision-making field.
Unveiling the Cultivation of Nostoc sp. under Controlled Laboratory Conditions
Authors: Mouga Teresa , Pereira Jéssica , Moreira Vitória , Afonso Clélia
Published: April 2024

Cyanobacteria, photoautotrophic Gram-negative bacteria, play a crucial role in aquatic and terrestrial environments, contributing significantly to fundamental ecological processes and displaying potential for various biotechnological applications. It is, therefore, critical to identify viable strains for aquaculture and establish accurate culture parameters to ensure an extensive biomass supply for biotechnology purposes. This study aims to establish optimal laboratory batch culture conditions for Nostoc 136, sourced from Alga2O, Coimbra, Portugal. Preliminary investigations were conducted to identify the optimal culture parameters and to perform biomass analysis, including protein and pigment content. The highest growth was achieved with an initial inoculum concentration of 1 g.L−1, using modified BG11 supplemented with nitrogen, resulting in a Specific Growth Rate (SGR) of 0.232 ± 0.017 μ.day−1. When exposed to white, red, and blue LED light, the most favourable growth occurred under a combination of white and red LED light exhibiting an SGR of 0.142 ± 0.020 μ.day−1. The protein content was determined to be 10.80 ± 2.09%. Regarding the pigments, phycocyanin reached a concentration of 200.29 ± 30.07 µg.mL−1, phycoerythrin 148.29 ± 26.74 µg.mL−1, and allophycocyanin 10.69 ± 6.07 µg.mL−1. This study underscores the influence of light and nutrient supplementation on the growth of the Nostoc biomass.
Seahorse-Tail-Inspired Soft Pneumatic Actuator: Development and Experimental Characterization
Authors: Antonelli Michele Gabrio , Beomonte Zobel Pierluigi , Sarwar Muhammad Aziz , Stampone Nicola
Published: April 2024

The study of bio-inspired structures and their reproduction has always fascinated humans. The advent of soft robotics, thanks to soft materials, has enabled considerable progress in this field. Over the years, polyps, worms, cockroaches, jellyfish, and multiple anthropomorphic structures such as hands or limbs have been reproduced. These structures have often been used for gripping and handling delicate objects or those with complex unknown a priori shapes. Several studies have also been conducted on grippers inspired by the seahorse tail. In this paper, a novel biomimetic soft pneumatic actuator inspired by the tail of the seahorse Hippocampus reidi is presented. The actuator has been developed to make a leg to sustain a multi-legged robot. The prototyping of the actuator was possible by combining a 3D-printed reinforcement in thermoplastic polyurethane, mimicking the skeletal apparatus, within a silicone rubber structure, replicating the functions of the external epithelial tissue. The latter has an internal channel for pneumatic actuation that acts as the inner muscle. The study on the anatomy and kinematic behaviour of the seahorse tail suggested the mechanical design of the actuator. Through a test campaign, the actuator prototype was characterized by isotonic tests with an external null load, isometric tests, and activation/deactivation times. Specifically, the full actuator distension of 154.5 mm occurs at 1.8 bar, exerting a maximum force of 11.9 N, with an activation and deactivation time of 74.9 and 94.5 ms, respectively.
Suppression of Contraction Raises Calcium Ion Levels in the Heart of Zebrafish Larvae
Authors: Martinez-Sielva Antonio , Vicente Manuel , Salgado-Almario Jussep , Garcia-Blazquez Aarón , Domingo Beatriz , Llopis Juan
Published: April 2024

Zebrafish larvae have emerged as a valuable model for studying heart physiology and pathophysiology, as well as for drug discovery, in part thanks to its transparency, which simplifies microscopy. However, in fluorescence-based optical mapping, the beating of the heart results in motion artifacts. Two approaches have been employed to eliminate heart motion during calcium or voltage mapping in zebrafish larvae: the knockdown of cardiac troponin T2A and the use of myosin inhibitors. However, these methods disrupt the mechano-electric and mechano-mechanic coupling mechanisms. We have used ratiometric genetically encoded biosensors to image calcium in the beating heart of intact zebrafish larvae because ratiometric quantification corrects for motion artifacts. In this study, we found that halting heart motion by genetic means (injection of tnnt2a morpholino) or chemical tools (incubation with para-aminoblebbistatin) leads to bradycardia, and increases calcium levels and the size of the calcium transients, likely by abolishing a feedback mechanism that connects contraction with calcium regulation. These outcomes were not influenced by the calcium-binding domain of the gene-encoded biosensors employed, as biosensors with a modified troponin C (Twitch-4), calmodulin (mCyRFP1-GCaMP6f), or the photoprotein aequorin (GFP-aequorin) all yielded similar results. Cardiac contraction appears to be an important regulator of systolic and diastolic Ca2+ levels, and of the heart rate.
Do Decision-Making Styles Predict Vagal Control? The Role of Resting Heart Rate Variability
Authors: Alacreu-Crespo Adrián , Costa Raquel , Molins Francisco , Abad-Tortosa Diana , SanMiguel Noemí , Courtet Philippe , Serrano Miguel Ángel
Published: April 2024

Decision-making styles are a habit-based propensity that drive behavior and affect daily life. Rational and intuitive decision-making styles have been associated with good mental health. However, the underlying mechanisms are not clear. In the last decade, high basal levels of heart rate variability (HRV) have been proposed as an index of health and emotional control, and this could be one of the variables involved in the effects of decision making on health. Therefore, the aim of this study is to analyze the capability of decision-making styles to predict resting HRV. A cross-sectional study was conducted in a sample of 199 (119 women) young university students, and a resting ECG was recorded to extract frequency domain HRV variables. Subsequently, participants completed sociodemographic data and the General Decision-Making Style questionnaire (GDMS). Results showed that the intuitive style predicted high-frequency HRV, while the avoidant style predicted less low-frequency HRV. This study presents new data on the relationship between decision-making style and HRV, suggesting that the intuitive style has a cardioprotective effect, while the avoidant style is related to lower HRV, which has been associated with health vulnerability. In conclusion, this study contributes to the understanding of HRV and its potential as a biomarker for cognitive styles that may improve health.
Exploring the Impact of a Supportive Work Environment on Chinese L2 Teachers’ Emotions: A Partial Least Squares-SEM Approach
Authors: Zeng Yonghong , Yu Jiaying , Wu Hanwei , Liu Wentao
Published: April 2024

Second language (L2) teachers’ emotions can influence their well-being and students’ performance. However, most of the existing studies have focused on the role of individual factors in affecting L2 teachers’ emotions, while leaving environmental factors underexplored. To fill this gap, this study aimed to examine how the four dimensions of a supportive work environment (SWE) (perceived climate, PC; supervisory relationship, SR; peer group interaction, PGI; and perceived organization support, POS) relate to L2 teachers’ emotions (enjoyment, anxiety, pride, and anger). A sample of 406 Chinese L2 teachers completed two valid scales to measure their SWE and emotions. The data were analyzed by Partial Least Squares-Structural Equation Modeling (SEM) using Smart PLS 3 software. The results showed that (1) PC, PGI, and POS had a positive and significant effect on enjoyment, while SR had no significant effect; (2) PGI and POS had a negative and significant effect on anxiety, while PC and SR had no significant effect; (3) PGI had a positive and significant effect on pride, while the other three dimensions had no significant effect; and (4) POS had a negative and significant effect on anger, while the other three dimensions had no significant effect. The study concludes with some implications for L2 teachers’ education.
Green Roofs on Shipping Containers: How Substrate Thickness Affects Thermal Performance
Authors: Moura Cléo de Araújo , Freitas Bianca Botelho de , Alves Filho Ailton Pinto , Albuquerque Cyro
Published: April 2024

Green roofs have become a popular sustainable solution in urban areas, and in recent years, shipping containers have gained popularity as a sustainable alternative for housing. A promising proposal is to combine these two solutions. This research aims to analyze the thermal behavior of experimental modules of scale constructions. Four modules were constructed with different substrate thicknesses (4, 6, 8, and 12 cm) to verify the impact on thermal behavior and provide guidance for this technology. Additionally, another module was built without a green roof for control purposes. The indoor and outdoor air temperatures and humidities, soil moistures, and temperatures between green roof layers were recorded in a tropical climate in summer. The behavior was similar between the different thicknesses for the whole period but with significant differences in the indoor temperature amplitudes (13.8 °C for the thinner substrate, 9.7 °C for the thicker one, and 38.7 °C for the bare roof). This study also revealed considerable heat conduction between the side walls and the slab, which resulted in an upward heat flow to the substrate during a day with a clear sky, which is the opposite of what is observed in conventional roofs. During the night and rainy periods, temperatures tend to become closer between the roof’s layers when the substrate dissipates the energy absorbed throughout the day.
Wind-Induced Aerodynamic Effects on Set-Back Tall Buildings Using LES
Authors: Yu Nan-Ting , Li Wei-Hao , Zheng Yu , Yuan Wei-Bin
Published: April 2024

Set-back modification represents a dependable method to reducing the wind effects on high-rise buildings, but the flow mechanism and aerodynamic responses of set-back tall buildings have not been explored systematically. Using the square building as the benchmark model, six set-back building models with varying steps and sizes were chosen for numerical simulation in this study. The results of mean wind pressure and local wind force distribution, power spectral densities of base moment, flow field, and structural response of the proposed building models are compared and analysed using the Large Eddy Simulation (LES) technique. The results demonstrate that the aerodynamic performance of set-back tall buildings dramatically improved, with the actual optimization in the cross-wind direction being more significant. Generally, a single set-back measure can enhance the aerodynamic performance of high-rise buildings more effectively than a double set-back measure. The outcome of this study provides an aerodynamic design guide for set-back tall buildings.
Optimising Plate Thickness in Interlocking Inter-Module Connections for Modular Steel Buildings: A Finite Element and Random Forest Approach
Authors: Elsayed Khaled , Mutalib Azrul A. , Elsayed Mohamed , Azmi Mohd Reza
Published: April 2024

Interlocking Inter-Module Connections (IMCs) in Modular Steel Buildings (MSBs) have garnered significant interest from researchers. Despite this, the optimisation of plate thicknesses in such structures has yet to be extensively explored in the existing literature. Therefore, this paper focuses on optimising the thickness of interlocking IMCs in MSBs by leveraging established experimental and numerical simulation methodologies. The study developed various numerical models for IMCs with plate thicknesses of 4 mm, 6 mm, 10 mm, and 12 mm, all subjected to compression loading conditions. The novelty of this study lies in its comprehensive parametric analysis, which evaluates the slip prediction model. A random forest regression model, trained using the ‘TreeBagger’ function, was also implemented to predict slip values based on applied force. Sensitivity analysis and comparisons with alternative methods underscored the reliability and applicability of the findings. The results indicate that a plate thickness of 11.03 mm is optimal for interlocking IMCs in MSBs, achieving up to 8.08% in material cost reductions while increasing deformation resistance by up to 50.75%. The ‘TreeBagger’ random forest regression significantly enhanced slip prediction accuracy by up to 7% at higher force levels.
Research on Statistical Characteristics and Prediction Methods of Ferronickel Slag Pervious Concrete Performance with Different Sizes of Aggregate and Mixtures
Authors: Tang Zhongping , Peng Hua , Yi Shixiang , Feng Fan
Published: April 2024

In the exploration of sustainable construction materials, the application of ferronickel slag (FNS) in creating pervious concrete has been investigated, considering its potential to meet the dual requirements of mechanical strength and fluid permeability. To elucidate the statistical properties and models for predicting the performance of FNS-composited pervious concrete with different sizes of aggregates and mixtures, a series of experiments, including 54 kinds of mixtures and three kinds of aggregate, were conducted. The focus was on measuring the compressive strength and the permeability coefficient. The results indicate that the compressive strength of pervious concrete decreases with the increase in aggregate size, while the permeability coefficient increases with the increase in aggregate size. Through normalization, the variability of these properties was quantitatively analyzed, revealing coefficients of variation for the concrete’s overall compressive strength and the permeability coefficient at 0.166, 0.132, and 0.150, respectively. Predictive models were developed using machine learning techniques, such as Linear Regression, Support Vector Machines, Regression Trees, and Gaussian Process Regression. These models demonstrated proficiency in forecasting the concrete’s compressive strength and permeability coefficient.