bluuebunny/crossref_metadata_2025 huggingface dataset:
Mobilizing to Martyrdom: A Narrative Theory of High-Risk Mobilization
Authors: Pape Robert , Rovang Dana , Ruby Keven , Decety Jean
Published: September 2023

Why do some individuals engage in high-risk mobilization in support of foreign militant groups, willingly sacrificing their possessions and even their lives as “martyrs”, when there is no imminent threat to the community within which they live or strong ties to the communities for which they sacrifice? We develop a new theory of high-risk mobilization rooted in the use of narratives that explains high-risk mobilization and identify the revolutionary use of multiple martyrdom narratives among modern Islamic militant groups, especially a heroic narrative specifically tailored to Western audiences, that can explain the group’s broad appeal beyond traditional pools of recruits. We demonstrate the existence of the heroic narrative in the video propaganda of ISIS and Jabhat al-Nusra and apply our framework of narratives of martyrdom to the universe of ISIS recruitment videos. We conclude by discussing implications for scholarship on mobilization and counter-radicalization/recruitment policy.
Solar System Wave Packet and its Achievements
Authors: Soltani Abolfazl
Published: September 2023

The emptiness of the interplanetary space in the solar system from large objects is very thought-provoking. For example, Consider the distance between Earth and Venus. This distance is about 25 thousand times the total diameter of Earth and Venus. It's like placing two small gravels on either side of a football pitch. Now, it would be self-deception to imagine that the protoplanetary disk didn't have enough material to form dozens of other planets (at least the size of the Mars) in the wide space between Earth and Venus, and only these two tiny gravels formed in this huge pitch. Something must have cleaned the objects between Earth and Venus from the protoplanetary disk. Here we show that this thing is the oscillation of a huge standing wave packet, with the wavelength λ = 0.6 AU, in the young solar system.
Så kan lärare öka elevers trygghet på rasten
Authors: Forsberg Camilla , Horton Paul
Published: September 2023

Elever i årskurs tre till sex känner sig tryggare i skolan om lärare finns närvarande, är vaksamma och ingriper mot framförallt mobbning och ensamhet på rasten. För att öka elevers trygghet i skolan efterfrågar eleverna därför insatser i relation till rasten. En aspekt som elever efterfrågar för att öka deras trygghet på rasten är att lärare finns närvarande. Utöver att finnas närvarande på raster behöver lärare också vara vaksamma på vad som händer mellan elever. I synnerhet behöver lärare vara vaksamma på att ingen är utsatt för mobbning eller ensam. Dessutom menar eleverna att det inte räcker med att lärare är närvarande och ser vad som händer utan de behöver även behöver ingripa mot mobbning och ensamhet. Ibland behöver de ingripa mer än vad de gör idag för att öka elevers trygghet och få stopp på mobbning och ensamhet.
Recent advances in the repair of degenerative intervertebral disc for preclinical applications
Authors: Ying Yijian , Cai Kaiwen , Cai Xiongxiong , Zhang Kai , Qiu Rongzhang , Jiang Guoqiang , Luo Kefeng
Published: September 2023

The intervertebral disc (IVD) is a load-bearing, avascular tissue that cushions pressure and increases flexibility in the spine. Under the influence of obesity, injury, and reduced nutrient supply, it develops pathological changes such as fibular annulus (AF) injury, disc herniation, and inflammation, eventually leading to intervertebral disc degeneration (IDD). Lower back pain (LBP) caused by IDD is a severe chronic disorder that severely affects patients’ quality of life and has a substantial socioeconomic impact. Patients may consider surgical treatment after conservative treatment has failed. However, the broken AF cannot be repaired after surgery, and the incidence of re-protrusion and reoccurring pain is high, possibly leading to a degeneration of the adjacent vertebrae. Therefore, effective treatment strategies must be explored to repair and prevent IDD. This paper systematically reviews recent advances in repairing IVD, describes its advantages and shortcomings, and explores the future direction of repair technology.
Electrochemical detection of kynurenic acid in the presence of tryptophan with the carbon paste electrode modified with the flower-like nanostructures of zinc oxide doped with terbium
Authors: Amir Poursaeed Ali , Jahani Shohreh , Moradalizadeh Mehran , Shahidi Zandi Mehdi , Foroughi Mohammad Mehdi
Published: September 2023

With the help of a hydrothermal approach in this study, we could provide flower-like nanostructures (NSs) of zinc oxide (ZnO) doped with Tb (FL-NS Tb3+/ZnO). Then, FL-NS Tb3+/ZnO morphology was investigated by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), X-ray powder diffraction (XRD), and map analysis. The results revealed higher activity centers and porosity of this nanocomposite, which were followed by acceptable electrochemical function. Hence, it can be utilized for fabricating an electrochemical sensor with an appropriate response for the simultaneous determination of kynurenic acid (KYN) and tryptophan (TRP). However, as compared with the modified carbon paste electrode (FL-NS Tb3+/ZnO/CPE), the bare carbon paste electrode (BCPE) exhibited a weak response toward KYN and TRP but the modified electrode was followed by a high current response for KYN and TRP at a potential 0.35 and 0.809 V. Therefore, cyclic voltammetry (CV) was applied in optimal experimental conditions to study the electrochemical behaviors of KYN and TRP over the surface of the proposed modified electrode. Moreover, we used differential pulse voltammetry (DPV) for quantitative measurements. It was found that this new modified electrode linearly ranged from 0.001 to 700.0 μM, with detection limits of 0.34 nM and 0.22 nM for KYN and TRP, respectively. In addition, KYN and TRP in real samples can be analyzed by this sensor, with a recovery of 97.75%−103.6% for the spiked KYN and TRP in real samples.
Risk factors of gastrointestinal bleeding after cardiopulmonary bypass in children: a retrospective study
Authors: Li Zheng-Qing , Zhang Wei , Guo Zheng , Du Xin-Wei , Wang Wei
Published: September 2023

Background

During cardiac surgery that involved cardiopulmonary bypass (CPB) procedure, gastrointestinal (GI) system was known to be vulnerable to complications such as GI bleeding. Our study aimed to determine the incidence and risk factors associated with GI bleeding in children who received CPB as part of cardiac surgery.

Methods

This retrospective study enrolled patients aged <18 years who underwent cardiac surgery with CPB from 2013 to 2019 at Shanghai Children's Medical Center. The primary outcome was the incidence of postoperative GI bleeding in children, and the associated risk factors with postoperative GI bleeding episodes were evaluated.

Results

A total of 21,893 children who underwent cardiac surgery with CPB from 2013 to 2019 were included in this study. For age distribution, 636 (2.9%) were neonates, 10,984 (50.2%) were infants, and 10,273 (46.9%) were children. Among the 410 (1.9%) patients with GI bleeding, 345 (84.2%) survived to hospital discharge. Incidence of GI bleeding in neonates, infants and children were 22.6% (144/636), 2.0% (217/10,984) and 0.5% (49/10,273), respectively. The neonates (22.6%) group was associated with highest risk of GI bleeding. Patients with GI bleeding showed longer length of hospital stays (25.8 ± 15.9 vs. 12.5 ± 8.9, P < 0.001) and higher mortality (15.9% vs. 1.8%, P < 0.001). Multivariate logistic regression analysis showed that age, weight, complicated surgery, operation time, use of extracorporeal membrane oxygenation (ECMO), low cardiac output syndrome (LCOS), hepatic injury, artery lactate level, and postoperative platelet counts were significantly associated with increased risk of GI bleeding in children with congenital heart disease (CHD) pediatric patients that underwent CPB procedure during cardiac surgery.

Conclusion

The study results suggest that young age, low weight, long operation time, complicated surgery, use of ECMO, LCOS, hepatic injury, high arterial lactate level, and low postoperative platelet counts are independently associated with GI bleeding after CPB in children.
Composition of ex vivo perfusion solutions and kinetics define differential cytokine/chemokine secretion in a porcine cardiac arrest model of lung preservation
Authors: Radomsky Lena , Koch Achim , Olbertz Carolin , Liu Yongjie , Beushausen Kerstin , Keil Jana , Rauen Ursula , Falk Christine S. , Kühne Jenny F. , Kamler Markus
Published: September 2023

Background

Ex vivo lung perfusion (EVLP) uses continuous normothermic perfusion to reduce ischemic damage and to improve post-transplant outcomes, specifically for marginal donor lungs after the donation after circulatory death. Despite major efforts, the optimal perfusion protocol and the composition of the perfusate in clinical lung transplantation have not been identified. Our study aims to compare the concentration levels of cytokine/chemokine in different perfusion solutions during EVLP, after 1 and 9 h of cold static preservation (CSP) in a porcine cardiac arrest model, and to correlate inflammatory parameters to oxygenation capacities.

Methods

Following cardiac arrest, the lungs were harvested and were categorized into two groups: immediate (I-EVLP) and delayed EVLP (D-EVLP), after 1 and 9 h of CSP, respectively. The D-EVLP lungs were perfused with either Steen or modified Custodiol-N solution containing only dextran (CD) or dextran and albumin (CDA). The cytokine/chemokine levels were analyzed at baseline (0 h) and after 1 and 4 h of EVLP using Luminex-based multiplex assays.

Results

Within 4 h of EVLP, the concentration levels of TNF-α, IL-6, CXCL8, IFN-γ, IL-1α, and IL-1β increased significantly (P < 0.05) in all experimental groups. The CD solution contained lower concentration levels of TNF-α, IL-6, CXCL8, IFN-γ, IL-2, IL-12, IL-10, IL-4, IL-1RA, and IL-18 (P < 0.05) compared with those of the Steen solution. The concentration levels of all experimental groups have correlated negatively with the oxygenation capacity values (P < 0.05). Protein concentration levels did not reach statistical significance for I-EVLP vs. D-EVLP and CD vs. CDA solutions.

Conclusion

In a porcine cardiac arrest model, a longer period of CSP prior to EVLP did not result in an enhanced protein secretion into perfusates. The CD solution reduced the cytokine/chemokine secretion most probably by iron chelators and/or by the protecting effects of dextran. Supplementing with albumin did not further reduce the cytokine/chemokine secretion into perfusates. These findings may help in optimizing the preservation procedure of the lungs, thereby increasing the donor pool of organs.
Case report: Coronary atherosclerosis in a patient with long-standing very low LDL-C without lipid-lowering therapy
Authors: Mottola Giorgio , Welty Francine K. , Mojibian Hamid R. , Faridi Kamil F.
Published: September 2023

Background

ApoB-containing lipoproteins including low-density lipoprotein cholesterol (LDL-C) are necessary for the development of atherosclerosis, and lifelong exposure to low serum levels of LDL-C have been associated with a substantial reduction of cardiovascular risk. Although plaque regression has been observed in patients with serum LDL-C less than 70–80 mg/dl on lipid-lowering therapy, an LDL-C level under which atherosclerosis cannot develop has not been established.

Case presentation

In this case we describe a 60-year-old man with well-controlled diabetes mellitus and hypertension who presented to the hospital after an acute stroke likely due to an atrial myxoma discovered on imaging. A coronary computed tomography angiography scan performed in preparation for the planned surgical myxoma resection revealed an anomalous origin of the right coronary artery as well as evidence of nonobstructive coronary atherosclerosis in the right coronary and non-anomalous left coronary system. Despite not having ever been on any lipid-lowering therapy, this patient was found to have low LDL-C levels (<40 mg/dl) during this admission and on routine laboratory data collected over the prior 16 years. His family history strongly suggested heterozygous familial hypobetalipoproteinemia as a possible diagnosis.

Conclusions

This case illustrates that even long-standing, very low levels of LDL-C may be insufficient to completely prevent atherosclerosis and emphasizes the importance of primordial prevention of all cardiovascular risk factors.
Perspectives on social justice when becoming a teacher-researcher in the practicum: insights from physical education teacher education
Authors: Sanches Neto Luiz , Venâncio Luciana , Corsino Luciano Nascimento , Conceição Willian Lazaretti da , Vieira Ewerton Leonardo da Silva , Barreto Samara Moura , Freire Elisabete dos Santos , Filgueiras Isabel Porto , Garbett Dawn , Ovens Alan
Published: September 2023

Understanding the role teachers must play in fostering social justice is one of many tasks student teachers learn on practicum. This self-study is a collaborative endeavour between eight Brazilian teacher-researchers who work in multiple teaching positions and settings in different regions of the country. Our assumption within the broader research scope is that being a teacher-researcher has commonalities related to social justice whether teaching in K-12 schools or higher education. We have reconfigured a collaborative, four-stage methodological approach to practitioner research. In this paper, we investigate how one teacher-researcher and university professor – Luiz, the first author – has been transforming his practice towards social justice through the self-study of teacher education practices. His initial dilemma concerned his students’ practicum experiences. Through the prompting of his co-authors and critical friends, Luiz reflected on his decisions and assumptions. He presented his analysis to the group which generated the discussion which forms the basis of this paper. The contribution this paper makes is two-fold. One is to exemplify the rigorous method we used to generate alternative perspectives through self-study. The other is to explore the impact of deeply seated social injustices on our teacher education practices.
A systematic bibliometric analysis on the clinical practice of CGM in diabetes mellitus from 2012 to 2022
Authors: Kong Laixi , Deng Bei , Guo Maoting , Chen Mengjie , Wang Xiaoxia , Zhang Mingjiao , Tang Hongxia , Wang Qin , Yang Liwei , Xiong Zhenzhen
Published: September 2023

Background

Continuous glucose monitoring (CGM) has revolutionized diabetes management, but a comprehensive analysis of its clinical implementation is lacking. This study aims to explore CGM in diabetes practice over the past decade using bibliometric analysis. It will identify trends, research focal points, and provide a framework for future investigations.

Materials and methods

The Web of Science Core Collection (WOSCC) was utilized to acquire literature pertaining to the employment of continuous glucose monitoring (CGM) in diabetes that was published between the years 2012 and 2022, and to conduct a comprehensive analysis of the associated citation data. To achieve bibliometric visualization and analysis of the collated data, the bibliography package in the Rstudio(v.4.2.2), Citespace 6.2.R4, and VOS viewer were employed.

Results

A total of 3024 eligible publications were extracted from 91 countries, with the United States being the leading country in terms of the number of issued articles. Furthermore, the annual publication rate has shown a gradual increase during the past decade. Among the various journals in this field, DIABETES TECHNOLOGY & THERAPEUTICS was identified as the most highly cited one. Keyword clustering analysis of the extracted publications indicates that the research hotspots in the past decade have primarily focused on “continuous glucose monitoring”, “glycemic variability”, “type 1 diabetes”, “hypoglycemia”, and “glycemic control”. Moreover, the analysis of keyword emergence reveals that “Time In Range” and “Young Adult” represent the current research frontiers for the years 2012-2022.

Conclusion

The concept of Time in Range (TIR) has garnered considerable attention as a significant area of inquiry and an emerging research trend in the clinical practice of Continuous Glucose Monitoring (CGM) for Diabetes Mellitus. Moreover, recent investigations have demonstrated a growing focus on young adults with type 1 diabetes as the research population of interest. In the foreseeable future, research endeavors will persist in the pursuit of improving glycemic management among young adults through the utilization of continuous glucose monitoring (CGM) technology, while also delving into the examination of the Time in Range metric via supplementary clinical investigations.
Should we suspect primary aldosteronism in patients with hypokalaemic rhabdomyolysis? A systematic review
Authors: Díaz-López Everardo Josué , Villar-Taibo Rocio , Rodriguez-Carnero Gemma , Fernandez-Pombo Antia , Garcia-Peino Roberto , Blanco-Freire Manuel Narciso , Pena-Dubra Alberto , Prado-Moraña Teresa , Fernández-Xove Irea- , Pérez-Béliz Edurne , Cameselle-Teijeiro Jose Manuel , Hermida-Ameijeiras Alvaro , Martinez-Olmos Miguel Angel
Published: September 2023

Severe hypokalaemia causing rhabdomyolysis (RML) in primary aldosteronism (PA) is a rare entity, and only a few cases have been reported over the last four decades. This systematic review and case report aims to gather all published data regarding a hypokalaemic RML as presentation of PA in order to contribute to the early diagnosis of this extremely rare presentation. With the use of PubMed Central, EMBASE, and Google Scholar, a thorough internet-based search of the literature was conducted to identify articles and cases with RML secondary to hypokalaemia due to PA between June 1976 and July 2023. The case study concerns a 68-year-old male patient with hypokalaemic RML at presentation of PA. In the systematic review of the literature, 37 cases of RML secondary to hypokalaemia due to PA have been reported to date. In summary, the median age was 47.5 years, the male/female ratio was 17/21, all patients presented symptoms (weakness and/or myalgia), all the patients were hypertensive, and only four patients had complications with acute kidney injury (AKI). Although PA rarely presents with RML, it should be suspected when marked hypokalaemia and hypertension are also present. Early detection and management are essential to reduce the frequency of manifestations such as AKI.
DapBCH: a disease association prediction model Based on Cross-species and Heterogeneous graph embedding
Authors: Shi Wanqi , Feng Hailin , Li Jian , Liu Tongcun , Liu Zhe
Published: September 2023

The study of comorbidity can provide new insights into the pathogenesis of the disease and has important economic significance in the clinical evaluation of treatment difficulty, medical expenses, length of stay, and prognosis of the disease. In this paper, we propose a disease association prediction model DapBCH, which constructs a cross-species biological network and applies heterogeneous graph embedding to predict disease association. First, we combine the human disease–gene network, mouse gene–phenotype network, human–mouse homologous gene network, and human protein–protein interaction network to reconstruct a heterogeneous biological network. Second, we apply heterogeneous graph embedding based on meta-path aggregation to generate the feature vector of disease nodes. Finally, we employ link prediction to obtain the similarity of disease pairs. The experimental results indicate that our model is highly competitive in predicting the disease association and is promising for finding potential disease associations.
Antigen-driven T cell-macrophage interactions mediate the interface between innate and adaptive immunity in histidyl-tRNA synthetase-induced myositis
Authors: Reay Daniel P. , Tabib Tracy , Wang Ying , Oriss Timothy B. , Young Nicholas A. , Lafyatis Robert A. , Jarjour Wael N. , Clemens Paula R. , Ascherman Dana P.
Published: September 2023

Introduction

Previous work in humans has demonstrated that both innate and adaptive immune signaling pathways contribute to the pathogenesis of idiopathic inflammatory myopathy (IIM), a systemic autoimmune disease targeting muscle as well as extra-muscular organs. To better define interactive signaling networks in IIM, we characterized the cellular phenotype and transcriptomic profiles of muscle-infiltrating cells in our established murine model of histidyl-tRNA synthetase (HRS)-induced myositis.

Methods

Myositis was induced in wild type (WT) and various congenic/mutant strains of C57BL/6 mice through intramuscular immunization with recombinant HRS. Histopathological, immunohistochemical, flow cytometric, and transcriptomic assessments were used to characterize the functional relationship between muscle-infiltrating cell populations in these strains lacking different components of innate and/or adaptive immune signaling.

Results

RAG1 KO mice developed markedly reduced muscle inflammation relative to WT mice, demonstrating a key requirement for T cells in driving HRS-induced myositis. While the reduction of mononuclear cell infiltrates in CD4-Cre.MyD88fl/fl conditional knockout mice and OT-II TCR transgenic mice highlighted roles for both innate and TCR-mediated/adaptive immune signaling in T cells, diminished inflammation in Lyz2-Cre.MyD88fl/fl conditional knockout mice underscored the importance of macrophage/myeloid cell populations in supporting T cell infiltration. Single cell RNA sequencing-based clustering of muscle-infiltrating subpopulations and associated pathway analyses showed that perturbations of T cell signaling/function alter the distribution and phenotype of macrophages, fibroblasts, and other non-lymphoid cell populations contributing to HRS-induced myositis.

Discussion

Overall, HRS-induced myositis reflects the complex interplay between multiple cell types that collectively drive a TH1-predominant, pro-inflammatory tissue phenotype requiring antigen-mediated activation of both MyD88- and TCR-dependent T cell signaling pathways.
Case Report: High-dose steroid and IVIG successful treatment in a case of COVID-19-associated autoimmune encephalitis: a literature review
Authors: Liu Chi-Hung , Chiu Li-Chung , Lee Chih-Chun , Chan Tien-Ming
Published: September 2023

Autoimmune encephalitis is a rare but critical complication of COVID-19. The management of COVID-19-associated autoimmune encephalitis includes the use of steroids, intravenous immunoglobulin (IVIG), plasmapheresis, and monoclonal antibody therapy. This study presented a patient with critical COVID-19 autoimmune encephalitis who rapidly recovered after the initiation of corticosteroids and IVIG therapy. This study reviewed the current literature on the pathophysiological mechanisms, diagnosis, and management of COVID-19-associated autoimmune encephalitis.
Type III interferon exerts thymic stromal lymphopoietin in mediating adaptive antiviral immune response
Authors: Cao Luhong , Qian Weiwei , Li Wanlin , Ma Zhiyue , Xie Shenglong
Published: September 2023

Previously, it was believed that type III interferon (IFN-III) has functions similar to those of type I interferon (IFN-I). However, recently, emerging findings have increasingly indicated the non-redundant role of IFN-III in innate antiviral immune responses. Still, the regulatory activity of IFN-III in adaptive immune response has not been clearly reported yet due to the low expression of IFN-III receptors on most immune cells. In the present study, we reviewed the adjuvant, antiviral, antitumor, and disease-moderating activities of IFN-III in adaptive immunity; moreover, we further elucidated the mechanisms of IFN-III in mediating the adaptive antiviral immune response in a thymic stromal lymphopoietin (TSLP)-dependent manner, a pleiotropic cytokine involved in mucosal adaptive immunity. Research has shown that IFN-III can enhance the antiviral immunogenic response in mouse species by activating germinal center B (GC B) cell responses after stimulating TSLP production by microfold (M) cells, while in human species, TSLP exerts OX40L for regulating GC B cell immune responses, which may also depend on IFN-III. In conclusion, our review highlights the unique role of the IFN-III/TSLP axis in mediating host adaptive immunity, which is mechanically different from IFN-I. Therefore, the IFN-III/TSLP axis may provide novel insights for clinical immunotherapy.
New insight into the role of fibroblasts in the epithelial immune microenvironment in the single-cell era
Authors: Wang Liangzhe , Wang Bo , Kou Erwen , Du Lin , Zhu Yuanjie
Published: September 2023

The skin is exposed to environmental challenges and contains heterogeneous cell populations such as epithelial cells, stromal cells, and skin-resident immune cells. As the most abundant type of stromal cells, fibroblasts have been historically considered silent observers in the immune responses of the cutaneous epithelial immune microenvironment (EIME), with little research conducted on their heterogeneity and immune-related functions. Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) have overcome the limitations of bulk RNA sequencing and help recognize the functional and spatial heterogeneity of fibroblasts, as well as their crosstalk with other types of cells in the cutaneous EIME. Recently, emerging single-cell sequencing data have demonstrated that fibroblasts notably participate in the immune responses of the EIME and impact the initiation and progression of inflammatory skin diseases. Here, we summarize the latest advances in the role of fibroblasts in the cutaneous EIME of inflammatory skin diseases and discuss the distinct functions and molecular mechanisms of activated fibroblasts in fibrotic skin diseases and non-fibrotic inflammatory skin diseases. This review help unveil the multiple roles of fibroblasts in the cutaneous EIME and offer new promising therapeutic strategies for the management of inflammatory skin diseases by targeting fibroblasts or the fibroblast-centered EIME.
In vivo imaging of patients with chronic pruritus of unknown origin reveals partial sweat duct obstruction with partial itch resolution upon retinoid treatment
Authors: Lim Shi Yu Derek , Tan Yingrou , Zhang Yuning , Zhao Xiahong , Ng Lai Guan , Tey Hong Liang
Published: September 2023

Background

Chronic pruritus of unknown origin (CPUO) is poorly understood and lacks effective treatment options.

Objectives

We aimed to elucidate abnormalities in the sweat apparatus of patients with CPUO, and to assess efficacy and safety of treatment with systemic retinoids.

Methods

An initial case–control study included 20 affected patients and five healthy controls, for whom heat and sweating were induced, either through a standardized exercise protocol or ingestion of hot water. In vivo high-definition optical coherence tomography, whole-body starch-iodine testing, and skin biopsy for immunofluorescence staining were done to evaluate for sweat duct obstruction. A subsequent retrospective cohort analysis included 56 patients with CPUO, seen at an Itch subspecialty clinic of a single tertiary referral centre, who failed conventional treatments and were treated with isotretinoin and/or acitretin from May 2014 to November 2020. Treatment response to retinoids was defined as a sustained reduction in itch score of ≥2/10. Safety was assessed by proportion stopping treatment due to side effects.

Results

In vivo imaging in 19 (95%) patients revealed features of partial keratinaceous sweat duct obstruction with statistically significant luminal dilatation compared to controls. Immunofluorescence studies of three patients’ paired lesional/non-lesional biopsies revealed dermcidin accumulation within sweat glands coupled with dermcidin leakage in itchy skin. Fifty-six patients (mean [SD] age 55.2 [17.5] years, 69.6% male) were treated with systemic retinoids. Mean (SD) duration of itch was 116.3 (140.4) months and mean (SD) itch score was 8.2 (1.8). Forty-one (73.2%) initially received isotretinoin, and 15 (26.8%) acitretin. At three months, mean itch score reduced by 2.38 (95% CI -3.2 to −1.6, p < 0.0001). Thirty-eight (67.9%) had a sustained response. Eight (14.81%) achieved an itch score of 0 or 1, with four stopping treatment for a mean (SD) of 318.5 (291.2) days without relapse. Eight (14.3%) stopped or switched retinoid due to adverse effects, with similar incidences between both retinoids, the commonest being dryness.

Conclusion

Based on novel findings from physiological imaging studies identifying partial keratinaceous sweat duct obstruction in CPUO, we instituted systemic retinoid treatment to address the underlying pathology. In patients who failed conventional therapies, the treatment appears effective and safe.
Machine learning approaches in microbiome research: challenges and best practices
Authors: Papoutsoglou Georgios , Tarazona Sonia , Lopes Marta B. , Klammsteiner Thomas , Ibrahimi Eliana , Eckenberger Julia , Novielli Pierfrancesco , Tonda Alberto , Simeon Andrea , Shigdel Rajesh , Béreux Stéphane , Vitali Giacomo , Tangaro Sabina , Lahti Leo , Temko Andriy , Claesson Marcus J. , Berland Magali
Published: September 2023

Microbiome data predictive analysis within a machine learning (ML) workflow presents numerous domain-specific challenges involving preprocessing, feature selection, predictive modeling, performance estimation, model interpretation, and the extraction of biological information from the results. To assist decision-making, we offer a set of recommendations on algorithm selection, pipeline creation and evaluation, stemming from the COST Action ML4Microbiome. We compared the suggested approaches on a multi-cohort shotgun metagenomics dataset of colorectal cancer patients, focusing on their performance in disease diagnosis and biomarker discovery. It is demonstrated that the use of compositional transformations and filtering methods as part of data preprocessing does not always improve the predictive performance of a model. In contrast, the multivariate feature selection, such as the Statistically Equivalent Signatures algorithm, was effective in reducing the classification error. When validated on a separate test dataset, this algorithm in combination with random forest modeling, provided the most accurate performance estimates. Lastly, we showed how linear modeling by logistic regression coupled with visualization techniques such as Individual Conditional Expectation (ICE) plots can yield interpretable results and offer biological insights. These findings are significant for clinicians and non-experts alike in translational applications.
Understanding the transcriptomic response of Lactiplantibacillus pentosus LPG1 during Spanish-style green table olive fermentations
Authors: López-García Elio , Benítez-Cabello Antonio , Tronchoni Jordi , Arroyo-López Francisco Noé
Published: September 2023

Lactiplantibacillus pentosus (Lbp. pentosus) is a species of lactic acid bacteria with a great relevance during the table olive fermentation process, with ability to form non-pathogenic biofilms on olive epidermis. The objective of this work is to deepen into the genetic mechanisms of adaptation of Lpb. pentosus LPG1 during Spanish-style green table olive fermentations, as well as to obtain a better understanding of the mechanisms of adherence of this species to the fruit surface. For this purpose, we have carried out a transcriptomic analysis of the differential gene expression of this bacterium during 60 days of fermentation in both brine and biofilms ecosystems. In brines, it was noticed that a total of 235 genes from Lpb. pentosus LPG1 were differentially expressed during course of fermentation and grouped into 9 clusters according to time-course analysis. Transport and metabolism of carbohydrates and amino acids, energy production, lactic acid and exopolysaccharide synthesis genes increased their expression in the planktonic cells during course of fermentation. On the other hand, expression of genes associated to stress response, bacteriocin synthesis and membrane protein decreased. A total of 127 genes showed significant differential expression between Lpb. pentosus LPG1 planktonic (brine) and sessile (biofilms) cells at the end of fermentation process (60 days). Among the 64 upregulated genes in biofilms, we found genes involved in adhesion (strA), exopolysaccharide production (ywqD, ywqE, and wbnH), cell shape and elongation (MreB), and well as prophage excision. Deeping into the genetic bases of beneficial biofilm formation by Lpb. pentosus strains with probiotic potential will help to turn this fermented vegetable into a carrier of beneficial microorganisms to the final consumers.
Genomic epidemiology and characterization of Staphylococcus aureus isolates from raw milk in Jiangsu, China: emerging broader host tropism strain clones ST59 and ST398
Authors: Liu Hui , Ji Xing , Wang Heye , Hou Xiang , Sun Haichang , Billington Craig , Zhang Lili , Wang Xiaoye , Wang Ran
Published: September 2023

Staphylococcus aureus is highly pathogenic and can cause disease in both humans and domestic animals. The aim of this study was to investigate the genomic epidemiology of S. aureus isolates from raw milk in Jiangsu Province, China, to identify predominant lineages and their associated genomic and phenotypic characteristics. In this study, we identified 117 S. aureus isolates collected from 1,062 samples in Jiangsu Province between 2021 and 2022. Based on whole-genome sequencing (WGS) data from 117 S. aureus isolates, molecular analyses indicated CC1-ST1 (26.50%, 31/117), CC97-ST97 (18.80%, 22/117), CC398-ST398 (10.26%, 12/117), CC8-ST630 (7.69%, 9/117) and CC59-ST59 (2.56%, 3/117) were the major lineages. The prevalence of mecA-positive strains was 11.11%. Four methicillin-resistant S. aureus (MRSA) lineages were found, including MRSA-ST59-t172 (n = 3), OS-MRSA-ST398-t011 (n = 1), MRSA-ST630-t2196 (n = 2) and OS-MRSA-ST630-t2196 (n = 7). Phenotypic resistance to penicillin (30.77%, 36/117), ciprofloxacin (17.09%, 20/117) and erythromycin (15.38%, 18/117) was observed which corresponded with resistance genotypes. All of the isolates could produce biofilms, and 38.46% (45/117) of isolates had invasion rates in mammary epithelial cells (MAC-T) of greater than 1%. Interestingly, most biofilm-producing and invading isolates harbored ebp-icaA-icaB-icaC-icaR-clfA-clfB-fnbA-fnbB-sdrC-sdrD-sdrE-map-can (27.35%, 32/117) and ebp-icaA-icaB-icaC-icaD-icaR-clfA-clfB-fnbA-fnbB-sdrC-sdrD-sdrE-map (33.33%, 39/117) adherence-associated gene patterns and belonged to lineages CC1 and CC97, respectively. Virulence factor assays showed that 47.01% of the isolates contained at least enterotoxin genes. Isolates harboring the immune evasion cluster (IEC) genes (sea, sak, chp, and scn) were predominantly categorized as STs 464, 398, and 59. IEC-positive ST398 and ST59 isolates contained a very high proportion of virulence genes located on prophages, whereas most IEC-negative ST398 clade isolates carried broad-spectrum drug resistance genes. Meanwhile, the IEC-positive ST398 clade showed a close genetic relationship with isolates from the pork supply chain and hospital surgical site infections. MRSA-ST59 strains showed the closest genetic relationship with an isolate from quick-frozen products. High-risk livestock-associated strains ST398 and MRSA-ST59 were detected in raw milk, indicating a potential public health risk of S. aureus transmission between livestock and humans. Our study highlights the necessity for S. aureus surveillance in the dairy industry.
The intricate dance: host autophagy and Coxiella burnetii infection
Authors: Wang Tingting , Wang Chao , Li Chang , Song Lei
Published: September 2023

Q fever is a zoonotic disease caused by Coxiella burnetii, an obligatory intracellular bacterial pathogen. Like other intracellular pathogens, C. burnetii is able to survive and reproduce within host cells by manipulating host cellular processes. In particular, the relationship between C. burnetii infection and host autophagy, a cellular process involved in degradation and recycling, is of great interest due to its intricate nature. Studies have shown that autophagy can recognize and target intracellular pathogens such as Legionella and Salmonella for degradation, limiting their replication and promoting bacterial clearance. However, C. burnetii can actively manipulate the autophagic pathway to create an intracellular niche, known as the Coxiella-containing vacuole (CCV), where it can multiply and evade host immune responses. C. burnetii promotes the fusion of CCVs with lysosomes through mechanisms involving virulence factors such as Cig57 and CvpF. This review summarizes the latest findings on the dynamic interaction between host autophagy and C. burnetii infection, highlighting the complex strategies employed by both the bacterium and the host. A better understanding of these mechanisms could provide important insights into the development of novel therapeutic interventions and vaccine strategies against C. burnetii infections.
Uncovering the immune microenvironment and molecular subtypes of hepatitis B-related liver cirrhosis and developing stable a diagnostic differential model by machine learning and artificial neural networks
Authors: Zhang Shengke , Jiang Chenglu , Jiang Lai , Chen Haiqing , Huang Jinbang , Zhang Jieying , Wang Rui , Chi Hao , Yang Guanhu , Tian Gang
Published: September 2023

Background: Hepatitis B-related liver cirrhosis (HBV-LC) is a common clinical disease that evolves from chronic hepatitis B (CHB). The development of cirrhosis can be suppressed by pharmacological treatment. When CHB progresses to HBV-LC, the patient’s quality of life decreases dramatically and drug therapy is ineffective. Liver transplantation is the most effective treatment, but the lack of donor required for transplantation, the high cost of the procedure and post-transplant rejection make this method unsuitable for most patients.

Methods: The aim of this study was to find potential diagnostic biomarkers associated with HBV-LC by bioinformatics analysis and to classify HBV-LC into specific subtypes by consensus clustering. This will provide a new perspective for early diagnosis, clinical treatment and prevention of HCC in HBV-LC patients. Two study-relevant datasets, GSE114783 and GSE84044, were retrieved from the GEO database. We screened HBV-LC for feature genes using differential analysis, weighted gene co-expression network analysis (WGCNA), and three machine learning algorithms including least absolute shrinkage and selection operator (LASSO), support vector machine recursive feature elimination (SVM-RFE), and random forest (RF) for a total of five methods. After that, we constructed an artificial neural network (ANN) model. A cohort consisting of GSE123932, GSE121248 and GSE119322 was used for external validation. To better predict the risk of HBV-LC development, we also built a nomogram model. And multiple enrichment analyses of genes and samples were performed to understand the biological processes in which they were significantly enriched. And the different subtypes of HBV-LC were analyzed using the Immune infiltration approach.

Results: Using the data downloaded from GEO, we developed an ANN model and nomogram based on six feature genes. And consensus clustering of HBV-LC classified them into two subtypes, C1 and C2, and it was hypothesized that patients with subtype C2 might have milder clinical symptoms by immune infiltration analysis.

Conclusion: The ANN model and column line graphs constructed with six feature genes showed excellent predictive power, providing a new perspective for early diagnosis and possible treatment of HBV-LC. The delineation of HBV-LC subtypes will facilitate the development of future clinical treatment of HBV-LC.
Risk of hospitalization in synucleinopathies and impact of psychosis
Authors: Piat Capucine , Mullan Aidan F. , Stang Cole D. , Hajeb Mania , Camerucci Emanuele , Turcano Pierpaolo , Martin Peter R. , Bower James H. , Savica Rodolfo
Published: September 2023

Background

Few studies have investigated the risk of hospitalization among patients with synucleinopathies (Parkinson disease, Dementia with Lewy Bodies, Parkinson disease dementia, Multiple System Atrophy) with associated psychosis and the impact of antipsychotic treatments on hospital admissions and duration of the stay.

Objective

To determine the risk of hospitalization among patients with synucleinopathies and in patients with associated psychosis. To evaluate the impact of antipsychotic treatments on hospital admission of patients with synucleinopathies and psychosis in an incident cohort study in Olmsted County, Minnesota (MN).

Methods

We used the Rochester Epidemiology Project (REP) to define an incident cohort of patients with clinically diagnosed synucleinopathies (1991–2010) in Olmsted County, MN. A movement disorder specialist reviewed all medical records to confirm the clinical diagnosis of synucleinopathies using the NINDS/NIMH unified diagnostic criteria.

Results

We included 416 incident cases of clinically diagnosed synucleinopathies from 2,669 hospitalizations. 409 patients (98.3%) were admitted to the hospital at least once for any cause after the onset of parkinsonism. The median number of hospitalizations for a single patient was 5. In total, 195 (46.9%) patients met the criteria for psychosis: patients with psychosis had a 49% (HR = 1.49, p < 0.01) increased risk of hospitalization compared to patients without psychosis. Among patients with psychosis, 76 (39%) received antipsychotic medication. Treatment with antipsychotic medications did not affect the risk of hospitalization (HR = 0.93, p = 0.65). The median length of hospitalization among the entire cohort was 1 (IQR 0–4) day. There was no difference between hospitalization length for patients with no psychosis and patients with active psychosis (RR = 1.08, p = 0.43) or patients with resolved psychosis (RR = 0.79, p = 0.24).

Conclusion

Psychosis increases the risk of hospitalization in patients with clinically defined synucleinopathies; however, it does not affect the length of hospital stays in our cohort. Antipsychotic treatment does not affect the risk of hospitalization in our study.
Longitudinal changes in attention bias to infant crying in primiparous mothers
Authors: Hiraoka Daiki , Makita Kai , Sakakibara Nobuko , Morioka Shigemi , Orisaka Makoto , Yoshida Yoshio , Tomoda Akemi
Published: September 2023

Introduction

Infant stimuli attract caregiver attention and motivate parenting behavior. Studies have confirmed the existence of attentional bias toward infant face stimuli; however, relatively little is known about whether attentional bias exists for infant cry stimuli, which are as important as faces in child-rearing situations. Furthermore, scarce longitudinal evidence exists on how attentional bias toward infant crying changes through the postpartum period.

Methods

In the present study, we conducted an experiment to assess bias toward infant crying at two postpartum time points: at Time 1 (Mean = 75.24 days), 45 first-time mothers participated and at Time 2 (Mean = 274.33 days), 30 mothers participated. At both time points, the mothers participated in a Stroop task with infant crying and white noise as the stimuli. They were instructed to answer the color out loud as quickly and accurately as possible, while ignoring the sound. Four types of audio stimuli were used in this task (the cry of the mother’s own infant, the cry of an unfamiliar infant, white noise matched to the cry of the mother’s own infant, and white noise matched to the cry of an unfamiliar infant), one of which was presented randomly before each trial. Response time and the correct response rate for each condition were the dependent variables.

Results

For response time, the main effect of familiarity was significant, with longer response times when the participant’s infant’s cry was presented. In addition, response times were lower at Time 2 than at Time 1 in some conditions in which crying was presented.

Discussion

The results suggest that mothers may be less disturbed by infant crying as they gain more experience. Elucidating the characteristics of postpartum mothers’ changes in cognitive performance related to infants’ cries would be useful in fundamental and applied research to understand the process of parents’ adaptation to parenting.
Research on obstacle avoidance optimization and path planning of autonomous vehicles based on attention mechanism combined with multimodal information decision-making thoughts of robots
Authors: Wu Xuejin , Wang Guangming , Shen Nachuan
Published: September 2023

With the development of machine perception and multimodal information decision-making techniques, autonomous driving technology has become a crucial area of advancement in the transportation industry. The optimization of vehicle navigation, path planning, and obstacle avoidance tasks is of paramount importance. In this study, we explore the use of attention mechanisms in a end-to-end architecture for optimizing obstacle avoidance and path planning in autonomous driving vehicles. We position our research within the broader context of robotics, emphasizing the fusion of information and decision-making capabilities. The introduction of attention mechanisms enables vehicles to perceive the environment more accurately by focusing on important information and making informed decisions in complex scenarios. By inputting multimodal information, such as images and LiDAR data, into the attention mechanism module, the system can automatically learn and weigh crucial environmental features, thereby placing greater emphasis on key information during obstacle avoidance decisions. Additionally, we leverage the end-to-end architecture and draw from classical theories and algorithms in the field of robotics to enhance the perception and decision-making abilities of autonomous driving vehicles. Furthermore, we address the optimization of path planning using attention mechanisms. We transform the vehicle's navigation task into a sequential decision-making problem and employ LSTM (Long Short-Term Memory) models to handle dynamic navigation in varying environments. By applying attention mechanisms to weigh key points along the navigation path, the vehicle can flexibly select the optimal route and dynamically adjust it based on real-time conditions. Finally, we conducted extensive experimental evaluations and software experiments on the proposed end-to-end architecture on real road datasets. The method effectively avoids obstacles, adheres to traffic rules, and achieves stable, safe, and efficient autonomous driving in diverse road scenarios. This research provides an effective solution for optimizing obstacle avoidance and path planning in the field of autonomous driving. Moreover, it contributes to the advancement and practical applications of multimodal information fusion in navigation, localization, and human-robot interaction.