bluuebunny/crossref_metadata_2025 huggingface dataset:
Developmental validation of the AGCU YNFS Y Kit: A new 6-dye multiplex system with 44 Y-STRs and 5 Y-InDels for forensic application
Authors: Sun Chaoran , Wang Xindi , Wang Shuangshuang , Zhou Yuxiang , Jiang Lanrui , Wang Zefei , Yao Hewen , Zhang Zhirui , Zha Lagabaiyila , Luo Haibo , Song Feng
Published: August 2024

With the widespread use of the Y chromosome in genetics, a lot of commercially available Y chromosome kits were developed, validated, and applied to forensic science practice. The AGCU YNFS Y Kit is a new Y chromosome system containing forty-four preferred Y short tandem repeats (Y-STRs) and five common Y-InDels. In this study, the AGCU YNFS Y system was validated to verify its performance by following the guidelines of the Scientific Working Group on DNA Analysis Methods (SWGDAM). A series of validation experiments included the following parameters: PCR-based studies, sensitivity studies, species specificity studies, stability studies, mixture studies, precision studies, stutter calculation, mutation and statistical analysis, population study, and case samples and degradation studies. The results suggested that appropriately changing PCR amplification conditions did not affect genotyping; the kit had good sensitivity for trace amounts of DNA (0.0625 ng), mixtures of multiple male individuals (minor: major = 1: 9), and three PCR inhibitors (more than 250 μM hematin, 250 ng/μL humic acid and 50 ng/μL tannic acid). The maximum standard deviation of allele size did not exceed 0.1552 reflecting the high accuracy of the system. By this, 87 DNA-confirmed pairs of father-son pairs were also analyzed for mutations. A total of 18 loci were mutated, with mutation rates ranging from 11.5×10−3 to 34.5×10−3 (95% CI 7.2×10−3–97.5×10−3, DYS627 and DYF404S1). In the population study, the haplotype diversity of 87 unrelated individuals was 0.9997, and discrimination capacity was 0.9885. Degradation studies have demonstrated that UV-C light exposure for up to 120 hours has no effect on male blood and semen-vaginal secretion mixtures. However, complete typing could no longer be obtained after 48 hours of UV exposure in single male saliva and in male saliva and female blood mixed samples. Collectively, the AGCU YNFS Y Kit is sensitive and accurate and can play its application value in forensic science practice.
In vitro fish mucosal surfaces producing mucin as a model for studying host-pathogen interactions
Authors: Quintana-Hayashi Macarena P. , Thomsson Hulthe Kristina A. , Lindén Sara K.
Published: August 2024

Current prophylactic and disease control measures in aquaculture highlight the need of alternative strategies to prevent disease and reduce antibiotic use. Mucus covered mucosal surfaces are the first barriers pathogens encounter. Mucus, which is mainly composed of highly glycosylated mucins, has the potential to contribute to disease prevention if we can strengthen this barrier. Therefore, aim of this study was to develop and characterize fish in vitro mucosal surface models based on commercially available cell lines that are functionally relevant for studies on mucin regulation and host-pathogen interactions. The rainbow trout (Oncorhynchus mykiss) gill epithelial cell line RTgill-W1 and the embryonic cell line from Chinook salmon (Oncorhynchus tshawytscha) CHSE-214 were grown on polycarbonate membrane inserts and chemically treated to differentiate the cells into mucus producing cells. RTGill-W1 and CHSE-214 formed an adherent layer at two weeks post-confluence, which further responded to treatment with the γ-secretase inhibitor DAPT and prolonged culture by increasing the mucin production. Mucins were metabolically labelled with N-azidoacetylgalactosamine 6 h post addition to the in vitro membranes. The level of incorporated label was relatively similar between membranes based on RTgill-W1, while larger interindividual variation was observed among the CHSE in vitro membranes. Furthermore, O-glycomics of RTgill-W1 cell lysates identified three sialylated O-glycans, namely Galβ1-3(NeuAcα2–6)GalNAcol, NeuAcα-Galβ1-3GalNAcol and NeuAcα-Galβ1-3(NeuAcα2–6)GalNAcol, resembling the glycosylation present in rainbow trout gill mucin. These glycans were also present in CHSE-214. Additionally, we demonstrated binding of the fish pathogen A. salmonicida to RTgill-W1 and CHSE-214 cell lysates. Thus, these models have similarities to in vivo mucosal surfaces and can be used to investigate the effect of pathogens and modulatory components on mucin production.
Experiences of smoking and tobacco use during pregnancy: A qualitative study protocol
Authors: Agràs-Guàrdia Maria , Martínez-Torres Sara , Satué Eva , Granado-Font Ester , Pallejà-Millán Meritxell , Patricio Demetria , Leiva Miriam , Rey-Reñones Cristina , Martín-Luján Francisco
Published: August 2024

Tobacco use during pregnancy is the main avoidable cause of morbidity and mortality both for pregnant women and their offspring. Between 12 and 22% of pregnant women in industrialized countries smoke during pregnancy, and 13% are unable to stop smoking. Pregnancy is considered an ideal opportunity to intervene and control tobacco use among smokers and their families. However, pregnant women experience barriers to quitting smoking, including social stigma and fear of being judged. Thus, it is necessary to develop interventions for smoking cessation adapted for pregnant women. This paper presents a qualitative study protocol to assess the barriers and facilitators of smoking cessation during pregnancy that female smokers encounter or perceive. It consists of a series of focus groups and individual interviews with female smokers who have been pregnant within the last five years. Participants will be recruited from the Sexual and Reproductive Health Care Services of the Camp de Tarragona. A group of 5–10 women who have been pregnant and tried to quit smoking over the last 5 years will be selected. The data will be collected by means of semistructured interviews. All interviews will be transcribed verbatim, coded and synthesized into categories and main themes. Thematic analysis will be conducted employing an iterative and reflexive approach. The results of this study will offer new perspectives on smoking interventions for pregnant women and enhance our comprehension of the main barriers to and facilitators of smoking cessation during pregnancy. This will contribute to the adaptation of the Tobbstop app, originally designed for the general public, to suit the needs of pregnant women. Consequently, the creation of targeted interventions will positively influence the health outcomes of both pregnant women and newborns.

Trial registration:Clinicaltrials.gov ID:NCT05222958. The trial was registered 3 February 2022, athttps://clinicaltrials.gov/ct2/show/NCT05222958.
EsperançArte – uma proposta da Psicologia Escolar para o Ensino Superior
Authors: Silva Silvia Maria Cintra da , Pessoa Camila Turati , Leite Gustavo Antônio Rodrigues , Cunha Marlon de Oliveira , Mascarin Sofia D Agostino
Published: August 2024

Em estreita e profícua interlocução com a Educação, a Psicologia Escolar e Educacional tem adentrado o campo do Ensino Superior, notadamente neste século. Neste artigo apresentamos uma proposta de Estágio em Psicologia Escolar, com a participação de estagiárias/os dos cursos de graduação em Pedagogia e Psicologia de uma universidade pública. O projeto, intitulado de EsperançArte, é voltado a ingressantes e propõe encontros permeados pela Arte, em que se discute a entrada na Universidade, a vida universitária, o compromisso social da profissão e outras temáticas relacionadas a esse momento. Fundamentado na Psicologia Histórico-Cultural e na Psicologia Escolar em uma vertente crítica, essa proposta mostra-se como uma alternativa para o enfrentamento à evasão, a atenção e o cuidado com a permanência e a conclusão do curso, considerando-se as condições de alienação e as contradições postas pela sociedade capitalista e vivenciadas pelas/os universitárias/os em seu cotidiano.
O curso de graduação em Medicina como ambiente, fonte de desenvolvimento: quais são seus significados?
Authors: Machado Clarisse Daminelli Borges , Schroeder Edson
Published: August 2024

Médicos-docentes são, entre outros atores institucionais, responsáveis pela formação técnica, ética e cognitiva dos estudantes de Medicina, transformando, assim, a docência médica em objeto de discussões ao longo das últimas décadas. As reflexões teóricas aqui apresentadas são desdobramentos de uma pesquisa de doutorado que se propôs a compreender a unidade “aprendizagem conduzindo ao desenvolvimento” dos estudantes de Medicina, por meio da atividade de ensino exercida pelos médicos-docentes, sob os aportes da Teoria Histórico-Cultural. Considerando o importante papel que estes desempenham na organização da relação mantida entre os estudantes e o conhecimento, nos diferentes ambientes de aprendizagem, se assevera que o ambiente tem papel decisivo no desenvolvimento de médicos-docentes assim como de estudantes, desde que, para tanto, seja reflexo de uma atmosfera científico-pedagógica compartilhada entre o curso de Medicina, os sujeitos nele inseridos e a Instituição de Ensino Superior. Se considera que tal atmosfera seja materializada no compartilhamento de ideais, na presença de uma intencionalidade pedagógica pautada em um campo teórico de base, pensada e desenvolvida de forma consciente e competentemente orientada para o desenvolvimento dos estudantes e, sobretudo, na atuação do conhecimento como mediador destas relações, transformando os diferentes espaços de aprendizagem em ambientes de desenvolvimento. Neste contexto é que se sustenta a importância do ambiente, enquanto fonte de desenvolvimento, nas relações dadas entre quem ensina e quem estuda.
Desafios à formação da consciência comunista no ensino superior: pensamento teórico e vontade de realização prática
Authors: Abrantes Angelo Antonio , Tuleski Silvana Calvo
Published: August 2024

O artigo objetiva apresentar as contribuições da Pedagogia Histórico-Crítica e da Psicologia Histórico Cultural ao trabalho educativo no ensino superior a partir das relações entre ensino, aprendizagem e desenvolvimento humano. Compreende-se que se a Pedagogia norteia a seleção de conteúdos e procedimentos didáticos, a Psicologia revela quem é concretamente o destinatário que se apropriará dos conteúdos, por meio dos procedimentos de ensino. A discussão baseia-se em dois movimentos de análise interligados; o primeiro focaliza o professor e sua atividade de ensino em sua dimensão político-pedagógica; o segundo focaliza o estudante pondo em destaque a formação da consciência, que se dá pelo movimento de apreensão das múltiplas determinações da realidade social, por meio da apropriação dos sistemas teórico-conceituais, destacando a atividade coletiva de estudo, pesquisa e intervenção nos âmbitos do ensino superior. Em ambos, docentes e discentes, a formação da “consciência revolucionária” em sua dimensão psicológica somente pode ser conhecida concretamente pela unidade entre consciência social e consciência pessoal. Por isso defende-se que a educação superior necessita dar condições para que o estudante não apenas se aproprie de teorias explicativas sobre os objetos estudados, mas experimente a sua utilização a partir da incorporação dos sistemas conceituais, mobilizando teoricamente os processos psíquicos na decifração e intervenção na realidade.
Seasonal variation in chemical compositions and biological activities of essential oils extracted from Ageratum conyzoides L. - grown in Western Ghats Region-South India
Authors: Karthigai Priya M , Poonkodi K , Ragupathi C , Rathinam R , Jayanthi E
Published: August 2024

The study aimed to characterize the influence of seasonal variation on the chemical composition and biological activities of essential oils extracted from the leaves of Ageratum conyzoides L. (A. conyzoides). Essential oils were extracted in summer (May), winter (January), and autumn (September) by hydrodistillation. GC/MS identified the volatiles compounds. The major compounds present in the essential oils across the three different seasons were Precocene II (29.44, 39.8 and 43.90%), Precocene I (32.17, 22.8 and 20.14%), and Caryophyllene oxide (9.09, 11.50 and 10.2%), followed by Carvone Hydrate (4.39, 4.65 and 3.65%) and Germacrene D (2.42, 2.25 and 2.76%). Biological activities such as in-vitro antioxidant, anti-cancer, and anti-bacterial activities were evaluated. The essential oil from A. conyzoides leaves exhibited strong antioxidant activity with IC50 values of 34.17 µg/mL for DPPH and 33.47 µg/mL for ABTS assays in summer; 99.92 µg/mL for DPPH and 26.40 µg/ mL for ABTS in winter; and 96.47 µg/mL for DPPH and 78.59 µg/mL for ABTS in autumn. The essential oil showed significant anticancer activity against the MDA-MB-231 breast cancer cell line with IC50 value of 122±0.05 µg/mL in summer, 86.50±0.05 µg/mL in winter, and 148.38 ± 1.15 µg/mL in autumn, compared to and Etoposide at 30 µg/mL. The antibacterial activity against two gram-positive and four Gram-negative bacteria is moderate to good.
Numerical solution of general order Emden-Fowler-type Pantograph delay differential equations
Authors: Albalawi Kholoud Saad , Kumar Ashish , Alqahtani Badr Saad , Goswami Pranay
Published: August 2024

The present study introduces the Haar wavelet method, which utilizes collocation points to approximate solutions to the Emden-Fowler Pantograph delay differential equations (PDDEs) of general order. This semi-analytic method requires the transformation of the original differential equation into a system of nonlinear differential equations, which is then solved to determine the Haar coefficients. The method’s application to fourth-, fifth-, and sixth-order PDDEs is discussed, along with an examination of convergence that involves the determination of an upper bound and the formulation of the rate of convergence for the method. Numerical simulations and error tables are presented to demonstrate the effectiveness and precision of this approach. The error tables clearly illustrate that the method’s accuracy improves progressively with increasing resolution.
Cloning operations and graph diameter
Authors: Iordanskiy Mikhail A.
Published: August 2024

The influence of the subgraph cloning operation on the graph diameter is studied. The corresponding potential increase in the diameter is estimated. Conditions under which the subgraph cloning operation causes no change in the graph diameter are formulated. An example of using the cloning operation to construct a family of fat trees is presented. The diameter of such graphs and the complexity of their design are estimated.
Relations between energy complexity measures of Boolean networks and positive sensitivity of Boolean functions
Authors: Mestetskiy Mikhail A. , Shupletsov Mikhail S.
Published: August 2024

We study relationships between lower estimates for the energy complexity E(Σ), the switching complexity S(Σ) of a normalized Boolean network Σ, and the positive sensitivity ps(f) of the Boolean function f implemented by this circuit. The lower estimate

E
(

Σ

)
⩾
⌊

ps
(
f
)
−
1

m

⌋

$\begin{array}{}
\displaystyle
E({\it\Sigma})\geqslant \lfloor\frac{\text{ps}(f)-1}{m}\rfloor
\end{array}$

is proved for a sufficiently wide class of bases consisting of monotone Boolean functions of at most m variables, the negation gate, and the Boolean constants 0 and 1. For the switching complexity of circuits, we construct a counterexample which shows that, for the standard basis of elements of the disjunction, conjunction, and negation, there do not exist a linear (with respect to ps(f)) lower estimate for the switching complexity.
On the maximal size of tree in a random forest
Authors: Pavlov Yuriy L.
Published: August 2024

Galton-Watson forests consisting of N rooted trees and n non-root vertices are considered. The distribution of the forest is determined by that of critical branching process with infinite variance and regularly varying tail of the progeny distribution. We prove limit theorem for the maximal size of a tree in a forest as N, n → ∞ in such a way that n/N → ∞. Our conditions are significantly wider than was previously known.
Probability that given vertices belong to the same connected component of random equiprobable mapping
Authors: Zubkov Andrey M. , Khalipov Petr V.
Published: August 2024

The random equiprobable mappings of finite set S into itself are considered. The probability that k fixed elements of S belong to the same connected component is studied. The limit of this probability as |S| → ∞ is found.
On analysing fragments: the case of No?
Authors: Wallage Phillip , van der Wurff Wim
Published: August 2024

This paper addresses the analysis of sentence fragments, specifically the English negative polar response item no. Two main types of synchronic analysis have been proposed for present-day English – one in which yes and no are syntactically inert particles which substitute for a clause, the other in which they are the initial element of an elided clause. Using diachronic data from 15th- to 17th-century English, we argue that the emergence of a novel other-speaker question pattern involving no demonstrates that speakers of early English analysed interrogative polar no as the initial element of a clause with TP-ellipsis. This novel pattern has received little attention in the literature, yet this grammatical innovation is interesting because its emergence demonstrates how diachronic change can be used as a diagnostic for underlying grammatical structure.
Exploring the anti-lung cancer mechanism of Ganoderma lucidum and its relationship with the level of immune cell infiltration based on network pharmacology and molecular docking
Authors: Luo Yuanyuan , Luo Xuehua , Xue Zhijian , Wu Miao , Chen Qiufang , Jin Ling
Published: August 2024

Objectives

Ganoderma lucidum has anti-tumor effects, but its mechanism of action against lung cancer is not clear. This study aims to use molecular docking and network pharmacology to investigate how G. lucidum inhibits tumor growth in lung cancer and how this relationship relates to the degree of immune cell infiltration.

Methods

By utilizing the TCMSP database, the therapeutic targets and active ingredients of G. lucidum were discovered. Based on the targets found in subsequent rounds of screening, a protein interaction (PPI) network was constructed. Additionally, enrichment analysis was conducted using GO and the KEGG. The molecular docking was conducted with the help of AutoDock Tools and PyMOL. Finally, we investigated the relationships between immune cells and the commonalities between lung cancer and.

Results

The primary targets of G. lucidum’s anti-lung cancer activities, according to network pharmacology studies, are ADRB2, OPRM1, SLC6A4, and JUN. The primary components of the lung cancer fighter Ergosterol are ganoderal B, ganolucidic acid E, and beta-sitosterol. The molecular docking analysis revealed that beta-sitosterol had the highest degree of stability in its docking interaction with JUN. The immune infiltration analysis indicated a potential correlation between the biomarkers associated with G. lucidum treatment for lung cancer and the immune infiltrating cells.

Conclusions

G. lucidum combats lung cancer by targeting multiple components and signaling pathways, facilitating cancer cell apoptosis and interacting with immune responses. This elucidates potential avenues for future research into G. lucidum’s mechanisms in lung cancer therapy.
A ver (si): diacronía de un marcador del discurso en español
Authors: Sánchez Jiménez Santiago U.
Published: August 2024

In this work, the diachronic perspective is used to explain the coexistence in current Spanish of three constructions: a) syntactic structures like a ver (si) + complement, integrated into the sentence framework, b) non-sentence fragments like a ver and c) independent structures, like a ver si + sentence complement. Out-side the sentence framework, both non-sentence fragments and independent sen-tences fulfil pragmatic values (interactive) or discursive values (favoring the construction of discourse). To carry out this diachronic investigation, we start from the syntactic insubordination hypothesis (cf. Evans 2007; Evans/Watanabe 2016), assuming that independence structures are the result of their insubordination from the governing nucleus of the sentence framework. In the first place, according to the documentation provided by the Corpus del Diccionario histórico de la lengua española (CDH), it is verified when the integrated syntactic structures like a ver (si) + complement, which we call experimentation structures, begin to be documented and when they stabilize in the language. The body of documents is subsequently reviewed to verify the first cases of syntactic insubordination and to verify how these syntactic independence processes end up being conventionalized and start to function as procedural marks with pragmatic and discursive value.
Sensory and Perceptual Decisional Processes Underlying the Perception of Reverberant Auditory Environments
Authors: García-Lázaro Haydée G. , Teng Santani
Published: August 2024

Reverberation, a ubiquitous feature of real-world acoustic environments, exhibits statistical regularities that human listeners leverage to self-orient, facilitate auditory perception, and understand their environment. Despite the extensive research on sound source representation in the auditory system, it remains unclear how the brain represents real-world reverberant environments. Here, we characterized the neural response to reverberation of varying realism by applying multivariate pattern analysis to electroencephalographic (EEG) brain signals. Human listeners (12 males and 8 females) heard speech samples convolved with real-world and synthetic reverberant impulse responses and judged whether the speech samples were in a “real” or “fake” environment, focusing on the reverberant background rather than the properties of speech itself. Participants distinguished real from synthetic reverberation with ∼75% accuracy; EEG decoding reveals a multistage decoding time course, with dissociable components early in the stimulus presentation and later in the perioffset stage. The early component predominantly occurred in temporal electrode clusters, while the later component was prominent in centroparietal clusters. These findings suggest distinct neural stages in perceiving natural acoustic environments, likely reflecting sensory encoding and higher-level perceptual decision-making processes. Overall, our findings provide evidence that reverberation, rather than being largely suppressed as a noise-like signal, carries relevant environmental information and gains representation along the auditory system. This understanding also offers various applications; it provides insights for including reverberation as a cue to aid navigation for blind and visually impaired people. It also helps to enhance realism perception in immersive virtual reality settings, gaming, music, and film production.
The Legality of Land Ownership Right that Changes in Position and Size (Case Study of Natural Liquefaction Disaster in Palu City)
Authors: Maisa Maisa , Muliadi Muliadi , Windiahsari Windiahsari
Published: August 2024

This study endeavors to accomplish two primary objectives: firstly, to evaluate the legal status of land ownership rights impacted by the liquefaction natural disaster in Palu, particularly focusing on instances where there are alterations in position and dimensions. Secondly, it aims to scrutinize the actions undertaken by land rights holders to secure legal ownership of land affected by such changes, particularly through engagements with the Palu City National Land Agency. Employing a juridical-empirical approach, the research illuminates two pivotal findings: firstly, that natural disasters, including liquefaction, can induce substantial shifts in land position, potentially complicating the legality of ownership and precipitating disputes among neighboring landowners. Secondly, it reveals that land rights holders commonly interface with the National Land Agency to rectify alterations in land position and size, underscoring the imperative of prompt reporting to ensure accurate measurement and documentation. The study posits two recommendations: firstly, advocating for timely reporting to the National Land Agency by affected communities to facilitate precise measurement and documentation of land shifts. Secondly, advocating for governmental enactment of specialized regulations addressing land transfers, thereby augmenting legal certainty for affected communities.
Multiple promoter and enhancer differences likely contribute to augmented G6PC2 expression in human versus mouse pancreatic islet alpha cells
Authors: Martin Cyrus C , Oeser James K , Wangmo Tenzin , Flemming Brian P , Attie Alan D , Keller Mark P , O’Brien Richard M
Published: August 2024

G6PC2 encodes a glucose-6-phosphatase catalytic subunit that opposes the action of glucokinase in pancreatic islets, thereby modulating the sensitivity of insulin and glucagon secretion to glucose. In mice, G6pc2 is expressed at ~20-fold higher levels in β-cells than in α-cells, whereas in humans G6PC2 is expressed at only ~5-fold higher levels in β-cells. We therefore hypothesize that G6PC2 likely influences glucagon secretion to a greater degree in humans. With a view to generating a humanized mouse that recapitulates augmented G6PC2 expression levels in α-cells, we sought to identify the genomic regions that confer differential mouse G6pc2 expression in α-cells versus β-cells as well as the evolutionary changes that have altered this ratio in humans. Studies in islet-derived cell lines suggest that the elevated G6pc2 expression in mouse β-cells versus α-cells is mainly due to a difference in the relative activity of the proximal G6pc2 promoter in these cell types. Similarly, the smaller difference in G6PC2 expression between α-cells and β-cells in humans is potentially explained by a change in relative proximal G6PC2 promoter activity. However, we show that both glucocorticoid levels and multiple differences in the relative activity of eight transcriptional enhancers between mice and humans likely contribute to differential G6PC2 expression. Finally, we show that a mouse-specific non-coding RNA, Gm13613, whose expression is controlled by G6pc2 enhancer I, does not regulate G6pc2 expression, indicating that altered expression of Gm13613 in a humanized mouse that contains both the human promoter and enhancers should not affect G6PC2 function.
Aldosterone, mitochondria and regulation of cardiovascular metabolic disease
Authors: Tsai Cheng-Hsuan , Chen Zheng-Wei , Lee Bo-Ching , Liao Che-Wei , Chang Yi-Yao , Tsai Yan-Rou , Chou Chia-Hung , Wu Vin-Cent , Hung Chi-Sheng , Lin Yen-Hung
Published: August 2024

Aldosterone is a mineralocorticoid hormone involved in controlling electrolyte balance, blood pressure, and cellular signaling. It plays a pivotal role in cardiovascular and metabolic physiology. Excess aldosterone activates mineralocorticoid receptors, leading to subsequent inflammatory responses, increased oxidative stress, and tissue remodeling. Various mechanisms have been reported to link aldosterone with cardiovascular and metabolic diseases. However, mitochondria, responsible for energy generation through oxidative phosphorylation, have received less attention regarding their potential role in aldosterone-related pathogenesis. Excess aldosterone leads to mitochondrial dysfunction, and this may play a role in the development of cardiovascular and metabolic diseases. Aldosterone has the potential to affect mitochondrial structure, function, and dynamic processes, such as mitochondrial fusion and fission. In addition, aldosterone has been associated with the suppression of mitochondrial DNA, mitochondria-specific proteins, and ATP production in the myocardium through mineralocorticoid receptor, nicotinamide adenine dinucleotide phosphate oxidase, and reactive oxygen species pathways. In this review, we explore the mechanisms underlying aldosterone-induced cardiovascular and metabolic mitochondrial dysfunction, including mineralocorticoid receptor activation and subsequent inflammatory responses, as well as increased oxidative stress. Furthermore, we review potential therapeutic targets aimed at restoring mitochondrial function in the context of aldosterone-associated pathologies. Understanding these mechanisms is vital, as it offers insights into novel therapeutic strategies to mitigate the impact of aldosterone-induced mitochondrial dysfunction, thereby potentially improving the outcomes of individuals affected by cardiovascular and metabolic disorders.
Yüksek Dolduruculu Akışkan Kompozit Rezinlerin Çarklı Cila Sistemleri ile Parlatıldıktan Sonraki Yüzey Özellikleri ve Renk Değişimleri
Authors: Çalışkan Mehmet Ali , Değirmenci Alperen , Boyacıoğlu Hayal , Turkun Sebnem
Published: August 2024

Amaç: Yüksek dolduruculu akışkan kompozitlere uygulanan çarklı cila sistemlerinin yüzey pürüzlülüğüne, parlaklığına ve renk stabilitesine etkilerini değerlendirmektedir.

Gereç ve Yöntemler: Clearfil Majesty ES Flow (CMF), Omnichroma Flow (OMNF), G-aenial Universal Injectable (GUI) ile nanohibrit NeoSpectra (NS) kullanıldı. Toplam 96 disk hazırlandı ve Twist Dia (TWD) veya Nova Twist (NOV) ile cilalandı. Profilometre ile yüzey pürüzlülükleri, Glossmetre ile parlaklık ölçümleri yapıldı ve kahvede bekletildi. Renk ölçümleri her işlemden sonra spektrofotometre ile yapıldı. Her gruptan ikişer örneğin yüzeyleri, tüm aşamalar için taramalı elektron mikroskopta incelendi. Yüzey pürüzlülük ve parlaklık değerleri ANOVA, Tukey, Pearson testleri ile, renk değişimi Paired-T-test, Independent T-test, ANOVA ve Dunnett-t ile değerlendirildi (p
Comparement of Two Dıfferent Graft Materıals in Lateral Sinus Augmentetion: 2 Case Reports
Authors: Arı Ilgın , Adiloğlu Selen
Published: August 2024

The rehabilitation of edentulous posterior maxilla can be complicated, and additional surgical procedures can be needed. The sinus augmentation procedures are predictable methods for edentolous maxillary posterior region. There are many various graft options for procedure such as autogen, xenogen, allogen and alloplastic grafts. All materials are using at the sinus augmentation as bloc and particule form from past to present. Xenografts and autografts are the most preferable materials in sinus augmentation. It was still investigated that which one of the graft materials has the most successful results. In this presented case report two patient were presented.who has similar vertical bone deficiencies in posterior maxillary region due to sinus pneumatization with the applications and success rates of autogen and xenogen graft materials in the area.
Державна ґендерна політика як фактор формування та розвитку ґендерно збалансованого бізнесу
Authors: Тюріна Діна
Published: August 2024

Актуальність. Важливе значення для забезпечення ґендерної рівності у суспільстві має ґендерна збалансованість бізнесу, оскільки підприємництво є сферою зайнятості великої частки економічно активного населення країни.
Метою дослідження є обґрунтування основних напрямів державної ґендерної політики у сфері бізнесу на основі аналізу сутності та показників ґендерного балансу бізнес-структур.
Результати. У статті обґрунтовано багатовекторність впливу держави на суспільне відтворення на основі гендерного підходу, що забезпечує досягнення ґендерної збалансованості суспільства. Доведено, що формування та розвиток ґендерно збалансованого бізнесу є комплексним напрямом та формою реалізації державної ґендерної політики.
Охарактеризовано основні види бізнесу як об’єктів державного регулювання за критерієм ґендерної збалансованості: ґендерно-домінуючий, ґендерно-лояльний, ґендерно-інклюзивний бізнес.
Розкрито основні характеристики ґендерного балансу бізнесу: рівні можливості чоловіків та жінок щодо реалізації їх права на здійснення підприємницької діяльності та отримання підприємницького доходу, права на труд та гідну його оплату, інших прав, що закріплені у нормативних актах держави та забезпечують гендерну рівність у суспільстві. Визначено показники ґендерного балансу бізнесу: частки жінок і чоловіків у загальній кількості власників бізнесу, керівників підприємств, фахівців, службовців, робітників як найманих працівників бізнесу; показники трудової зайнятості жінок, гендерного розриву в оплаті праці.
Обґрунтовано основні завдання державного гендерного регулювання бізнесу – створення правових та соціально-економічних умов трансформації великого та середнього бізнесів у ґендерно лояльні та інклюзивні; підтримка гендерного балансу мікро- та малого підприємництва.
Висновки. Доведено, що заходи державного регулювання бізнесу повинні базуватися на ґендерно чутливому підході, ураховувати специфічні соціально-економічні, політичні, культурні та інші характеристики умов життя та потреб жінок і чоловіків як його власників та найманих працівників.
Perspectives of BrinquEinstein healthcare professionals on the implementation of therapeutic play in pediatrics
Authors: Miranda Carolline Billett , Maia Edmara Bazoni Soares , Almeida Fabiane de Amorim
Published: August 2024

Abstract The benefits of therapeutic play (TP) in pediatrics are widely reported in the literature, however its use by health professionals is still limited. The objective was to understand how professionals belonging to the BrinquEinstein group evaluate the process of systematic implementation of TP in hospital pediatric units. Exploratory study, with a qualitative approach, developed in the pediatric and intensive care units of extra-large general hospital in São Paulo. The sample consisted of 13 professionals from different categories belonging to BrinquEinstein. Data was collected through individual semi-structured and audio-recorded interviews, being analyzed based on the Inductive Thematic Analysis proposed by Braun and Clark. From the analysis of the interviews, five themes emerged: experiencing a transforming process; the benefits that strengthen the path; the facilities that encourage the walk; the barriers that challenge the process; the future prospects. For the interviewed professionals, it is essential that the use of TP becomes a routine practice in different contexts of the child´s healthcare, in which managers and institutions play a fundamental role in its implementation.
Experiencias de mujeres en la atención del parto institucional en tiempos de la primera y segunda ola de COVID en México
Authors: González Kassandra Daniela Ríos , Rangel-Flores Yesica Yolanda
Published: August 2024

Resumen Estudio de tipo cualitativo que explora las perspectivas y experiencias de un grupo de mujeres mexicanas que vivieron la atención institucionalizada del parto en la primera y segunda ola de la pandemia por COVID-19. A través de un guión semiestructurado se entrevistó a nueve mujeres que vivieron la experiencia de la atención del parto entre marzo y octubre de 2020, en hospitales públicos y privados de la ciudad de San Luis Potosí, en México. Bajo la propuesta de análisis de la teoría fundamentada, se identificó que las estrategias sanitarias implementadas en el marco de la pandemia, trajeron consigo un retroceso en la garantía del parto humanizado, las mujeres se narraron desconfiadas en los protocolos que siguió el personal para la atención de sus partos en los hospitales del sector público y muy confiadas en los que se implementaron en el sector privado. La realización de cesáreas sin una justificación clara emergió como una constante, igual que la separación temprana de los binomios. Continúa frágil la disposición y el convencimiento del personal sanitario y las instituciones para garantizar, proteger y defender el derecho de las mujeres a vivir el parto libre de violencia. Persisten resistencias para repensar la atención del parto desde un paradigma no biomédicalizante.
Perceptions of informal caregivers about motivations, needs, and benefits of care for dependent older adults
Authors: Capelo Maria Regina Teixeira Ferreira , Brasil Christina César Praça , Silva Rita Maria Lemos Baptista , Capelo João André Ferreira , Quintal António José de Olim Marote , Ribeiro Leonardo José Maciel , Silva Raimunda Magalhães , Oliveira Ellen Synthia Fernandes de
Published: August 2024

Abstract The informal caregiver provides non-remunerated permanent or regular care to dependent older adults. This qualitative study aimed to identify the perceptions of informal caregivers about motivations, needs, and benefits of caring for dependent older adults. It was conducted with ten Portuguese informal caregivers, based on an instrument with questions about the care provided to older adults and their perceptions about performing this role. The results revealed the following motivations for care: proximity and trust relationship, duty of care, more available family members, home proximity, lack of vacancies, high cost of shelter institutions, and older adults’ desire to remain in their homes. The primary care activities for older adults are hydration, hygiene, food, therapeutic administration, companionship, emotional support, comfort, entertainment, and promoting autonomy and dignity. The needs identified by the caregivers were home, social security, and the caregiver’s employer support, financial help, psychological support, and training to care for the older adults. The benefits of informal care for dependent older adults were prompt family support, physical and emotional security, affection, and companionship. This study gives voice to crucial citizens.
Portuguese version of the Short-Form Condom Attitude Scale and psychometric properties among undergraduate students
Authors: Zucoloto Miriane Lucindo , Silva Wanderson Roberto da , Silva Rafaella Alves , Prudencio Jonathan Leonardo Gonçalves , Frazatto Vitoria de Souza Pinto , Martinez Edson Zangiacomi
Published: August 2024

Abstract The condom use can be influenced by psychological and sociocultural aspects, which can be modulated by individual’s attitudes. The aim was to propose a Brazilian Portuguese version of the Short-Form Condom Attitude Scale (Short-Form CAS), describing all procedures of translation, transcultural adaptation, and psychometric properties evaluation when applied to undergraduate students. A cross sectional study was conducted among students enrolled at University of São Paulo (USP), Campus Ribeirão Preto (n = 491; 61.2% female; average age: 22 years; standard deviation: four years). Confirmatory factor analysis was performed. A one-factor model with seven items exhibited good factorial validity and reliability, suggesting to be a better factorial solution of the Short-Form CAS in the sample. Few studies have been carried out on the behavioral factors associated with condom use in key populations, which can be exacerbated by a lack of appropriate scales. Therefore, the main contribution of the present study was to propose a simplified and transculturally adapted version of the Short-form CAS with some psychometric properties verified. Since the use of condoms is a component of the study of sexual risk behavior, this scale might be an option to be applied in various Brazilian population segments for this purpose.
Perspectivas de estudantes e egressos sobre a aprendizagem baseada em problemas na formação de enfermeiros
Authors: Rodrigues Paula Sales , Marin Maria José Sanches , Souza Aline Pereira , Vernasque Juliana Ribeiro da Silva , Grandin Gabriela Martins , Almeida Karina Roberta Vieira de , Oliveira Carolina Saab Rocha de
Published: August 2024

Resumo Objetivou-se analisar a percepção de estudantes e egressos sobre a utilização da Aprendizagem Baseada em Problemas (ABP) na formação do enfermeiro. Trata-se de um estudo qualitativo que utiliza a modalidade compreensiva e interpretativa proposta pela Hermenêutica-Dialética. Realizaram-se quatro grupos focais com a participação de 17 estudantes e 16 egressos de uma instituição de ensino superior que aplica a ABP na formação de enfermeiros. A análise dos resultados permitiu a definição de cinco categorias temáticas: dificuldade de adaptação em relação ao método; conquista de autonomia sobre o próprio aprendizado; incentivo ao desenvolvimento do raciocínio clínico; aprimoramento da comunicação e das relações interpessoais e integração entre teoria e prática. Evidencia-se que a utilização da ABP favorece a aproximação com as proposições das diretrizes curriculares para a formação do enfermeiro por meio do desenvolvimento de habilidades e competências como autonomia, comunicação, relações interpessoais e raciocínio clínico mediante práticas integrais e contextualizadas. Entretanto, os estudantes enfrentam dificuldades com as mudanças observadas ao serem inseridos nela ABP, as quais são superadas no decorrer do processo de implementação.
Students’ and graduates’ perceptions on problem-based learning in nursing undergraduate education
Authors: Rodrigues Paula Sales , Marin Maria José Sanches , Souza Aline Pereira , Vernasque Juliana Ribeiro da Silva , Grandin Gabriela Martins , Almeida Karina Roberta Vieira de , Oliveira Carolina Saab Rocha de
Published: August 2024

Abstract This study aimed to analyze students’ and graduates’ perceptions regarding the use of Problem-Based Learning (PBL) in nurse education. This is a qualitative study that employs the comprehensive and interpretative approach proposed by Dialectical Hermeneutics. Four focus groups were conducted with the participation of 17 students and 16 graduates from a higher education institution that implements PBL in nurse education. The analysis of results allowed for the identification of five thematic categories: difficulty in adapting to the method; attainment of autonomy in one’s own learning; encouragement of clinical reasoning development; enhancement of communication and interpersonal relationships; and integration between theory and practice. It is evident that the use of PBL promotes alignment with the propositions of curriculum guidelines for nurse education by fostering the development of skills and competencies such as autonomy, communication, interpersonal relationships, and clinical reasoning through comprehensive and contextualized practices. However, students encounter challenges with the changes observed when introduced to PBL, which are overcome during the implementation process.
Perceções dos tutores sobre o papel do cão na dinâmica familiar durante a pandemia
Authors: Firmino Cristiana Isabel da Cruz Furtado , Pinto Filipa Alexandra Coelho , Varela Nídia Sofia Tavares , Westermann Débora Bianca Sancho , Longo João José Rolo , Fernandes Maria João Sousa
Published: August 2024

Resumo Com o confinamento imposto pelo SARS-CoV-2, houve mudanças na dinâmica familiar. Para os estudantes de enfermagem este foi um assunto que gerou interesse em investigar. Partindo da questão: Qual o papel do cão na dinâmica familiar em tempo de pandemia? e do objetivo: identificar as perceções dos tutores sobre o papel do cão na dinâmica familiar num período de confinamento, enveredámos por um estudo qualitativo, descritivo, com recurso a focus group e amostra de seis membros de famílias diferentes. O tratamento e análise de dados seguiu o protocolo de Bardin. Identificámos que a existência de cão numa família influencia a sua dinâmica através dos benefícios obtidos quer a nível da saúde mental e bem-estar psicológico, quer a nível da saúde física. Igualmente, destacaram-se vantagens, apesar de algumas desvantagens. O vínculo afetivo entre cão e família, advém da reflexão sobre legislação portuguesa, proteção dos direitos dos animais, responsabilidades dos tutores e configuração familiar. Concluímos que na enfermagem, é necessário relevar a presença do cão na família uma vez que traz benefícios para a sua dinâmica. Esta torna-se uma visão inovadora quando pretendemos contribuir para a manutenção da saúde familiar focada nas necessidades de cada indivíduo, família e comunidade.
Mental health care measures and innovations to cope with COVID-19: an integrative review
Authors: Silva Amanda Seraphico Carvalho Pereira da , Furtado Lumena Almeida Castro , Tofani Luís Fernando Nogueira , Bigal André Luiz , Bragagnolo Larissa Maria , Vieira Amanda da Cruz Santos , Lima Carolina Loyelo , Oliveira Letícia Bucioli , Chioro Arthur
Published: August 2024

Abstract This integrative review aims to identify the mental health care measures that were produced during the COVID-19 pandemic. This research was conducted on three databases (SciELO, PubMed, and LILACS) with the following descriptors in Portuguese, English, and Spanish: “SAÚDE MENTAL” or “SALUD MENTAL” or “MENTAL HEALTH” AND “COVID-19” from 2020 to 2021. In total, 3,451 articles were found, 43 of which were analyzed. Most measures were digital, stemmed from public institutions, focused on the local perspective, and were integrated with the public health care system. This study discusses the models of care in mental health based on measures to cope with the COVID-19 pandemic. It also discusses the Brazilian health care system, reiterating its resilience. In conclusion, digital measures occurred most often. This study suggest the evaluation of the accessibility of this mental health care model for most vulnerable groups. Finally, this research reinforces the importance of the Brazilian health care system for public health and access to information to cope with the COVID-19 pandemic.
Atenção infantil na rede de cuidados à pessoa com deficiência no Brasil: um estudo multicêntrico
Authors: Mendonça Ayrles Silva Gonçalves Barbosa , Oliveira Bianca Larissa Lesca de , Fernandes Tiótrefis Gomes , Barroso Ralf Braga , Ribeiro Kátia Suely Queiroz Silva , Schmitt Ana Carolina Basso
Published: August 2024

Resumo Objetivou-se identificar fatores associados à atenção à saúde infantil na atenção primária à saúde (APS), na perspectiva das ações preconizadas pela Rede de Cuidados à Pessoa Deficiência (RCPCD), quanto à identificação precoce de deficiências e acompanhamento do desenvolvimento infantil. Trata-se de estudo transversal, multicêntrico, realizado em oito estados brasileiros. A coleta de dados envolveu a aplicação de questionário estruturado com profissionais de saúde de nível superior atuantes na APS, com amostra aleatória estratificada por estado e município. Foram realizadas regressões de Poisson com variância robusta para duas variáveis resposta. Entre os 1.488 trabalhadores que compuseram a amostra final, 63,6% realizam ações de identificação precoce de deficiências e 49% efetuam acompanhamento do desenvolvimento infantil. As equipes de Saúde da Família (eSF) identificam e acompanham mais do que as equipes do modelo tradicional, e os Núcleos Ampliados de Saúde da Família e Atenção Básica realizam mais ações de acompanhamento do que essas equipes. Conclui-se que os fatores mais associados com a identificação e o acompanhamento de crianças com deficiências na APS foram aqueles relativos à formação profissional, à eSF e ao conhecimento de normativas relativas à RCPCD.
Adherence to antihypertensive drug treatment in Brazil: a systematic review and meta-analysis
Authors: Coelho Juliana Chaves , Guimarães Mayra Cristina da Luz Pádua , Vaz Ana Katly Martins Gualberto , Meira Karina Cardoso , Santos Juliano dos , Lee Renata Jae Won , Drager Luciano Ferreira , Pierin Angela Maria Geraldo
Published: August 2024

Abstract This article aims to evaluate the adherence to antihypertensive treatment prevalence in the Brazilian population based on peer-reviewed studies which used instruments exclusively designed and/or adapted for this purpose. A systematic review with meta-analysis based on the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The search was carried out in the BDENF, SciELO, Cuiden, PsycINFOe, CINAHL, Embase, LILACS, and MEDLINE databases, as well as the AgeLine, Google Scholar and ScienceDirect academic search engines. The protocol was registered with PROSPERO (CRD42021292689). Random effects models were used for a meta-analysis of the prevalence obtained from individual studies. A total of 104 studies were included in the meta-analysis on antihypertensive treatment in the Brazilian population, totaling 38,299 patients. The most used instrument was the four-item Morisky-Green Test (49.5%). The adherence prevalence estimated by the meta-analysis was 44.4% (95%CI: 39.12%-49.94%, I2 = 91.17, p < 0.001), showing high heterogeneity. The adherence to antihypertensive treatment prevalence found in national studies was unsatisfactory, demonstrating that this problem continues to be a major challenge.