bluuebunny/crossref_metadata_2025 huggingface dataset:
Phytochemical Screening, Isolation, and Structure Elucidation of the Radish (Raphanus sativus Linn.) Bulb Ethanolic Extract Using Gas Chromatography- Mass Spectrometry (GC-MS)
Authors: San Juan Ma. Eva C. , Jumala Ramchand S. , Niasca Karen Hope G.
Published: February 2016

Nowadays, plant is being used as a biofuel, a probable source for a pesticide and it is valued for its pharmacological effects. Hence, the researchers engaged in the scientific approach to provide explanation to these claims. The study focused on the phytochemical screening of the ethanolic extract of radish bulbs (Raphanus sativus). It further dealt on the isolation and determination of the molecular masses to provide probable structures and associated molecular properties for its constituents.
Alkaloids, flavonoids, saponins and tannins were analyzed. Further, this effort involved analysis through Thin Layer Chromatography (TLC) using the mobile phase of Toluene: Chloroform (9:11) and Gas Chromatography- Mass Spectrometry (GC-MS) to analyze the first spot (Retention Factor= 0.667).
Phytochemical analysis showed no tannins. Using TLC, it revealed two spots having Rf values of 0.667 and 0.507, respectively. Using GC-MS method, 24 different molecules were isolated and analyzed of which only 23 were given probable structures. The 23 molecules constituted 11 long-chain alkanes, 4 long-chain alkyl esters, 2 aromatic ketones, a benzyl halide, an aryl ether, a quinone, an aromatic aldehyde, a long chain aromatic alkane, and a long-chain alkyl nitrile. With this finding, the bulb extract may be a good source of potential biofuel (mono-alkyl ester), UV A, B and C blockers in the form of diphenyl-ketone, oviposition attractants (long-chain alkanes) and also a source of bioactive molecules such as estragole which shows antispasmodic effect, methyl palmitate with immunosuppressive effect in semi-allografts and hepatoprotective activity in rats, and an analogue of the antidepressant alfetamine.
Pre Clinical Evaluation of Lagnub (Ficus septica, Moraceae) Leaf Crude Extract
Authors: Nogodula Judee N. , Gran Preziel F. , Salamanca Lyn Venice I. , Yeo Ellaine Ann S.
Published: February 2016

Lagnub is popularly used by Ata Matigsalog in Arakan, North Cotabato in treating skin infections. Scientific investigation was done to prove its folkloric claim by determining the acute dermal toxicity level, mutagenic activity, antifungal property using Candida albicans ATCC and two clinical isolates and its Minimum Inhibitory Concentration (MIC). Acute dermal toxicity was performed using the fixed dose of dried leaves at 1000 mg/kg (Sighting study) and 1000, 2000 and 5000 mg/kg for the main study. The protocol was based on OECD guideline. Susceptibility test for antifungal activity was done using Kirby-Bauer Method. Two-fold agar dilution was followed for Minimum Inhibitory Concentration and Direct Method of Ames test was performed for mutagenicity. Results showed that the dried leaf crude extract was categorized as non-toxic because there were no deaths of rabbits or evident toxicity at the upper limit dose of 5000 mg/kg. However, Ames test revealed that the dried leaf extract was considered as mutagenic against Salmonella typhimurium TA98. Antifungal activity revealed that the leaf crude extract was interpreted as active having a zone of 16.67 mm ±5.3852 (C. albicans ATCC), clinical isolate 1 is 12.55 ±3.2974 mm, and clinical isolate 2 is 12.51 ±3.4278 mm. Statistically, there was a significant difference in the mean zones of inhibition exhibited by the plant material against the test organisms. Meanwhile, the minimum inhibitory concentration of lagnub was 52, 631.58 μg/mL
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Authors: Limjuco Renan P.
Published: February 2016

UIC RESEARCH JOURNAL

International Edition

Volume 18, Number 2, October 2012

 

Editor in Chief

Renan P. Limjuco

 

Associate Editor

Emma V. Sagarino

 

Managing Editor

Francis Kenneth D. Canono

 

Research Committee

Dr. Adorico M. Aya-ay

Engr. Noel V. Laud

Prof. Exander T. Barrios

Dr. Renan P. Limjuco

Dr. Alvin O. Cayogyog

Dr. Lucila T. Lupo

S. Ma. Luz O. de la Cruz, RVM

Dr. Cyril Cliffredson C. Mamocod

Prof. Eric John G. Emberda

Prof. Emma V. Sagarino

Dr. Elsa B. Faceronda

Dr. Ma. Eva C. San Juan

Dr. Ma. Teresa M. Gravino

S. Ma. Marissa R. Viri, RVM

 

Filipino Scholar Reviewers

Engr. Ruth P. Barluado

Prof. Alma R. Lamparas

Dr. Marleonie M. Bauyot

Prof. Ivan Anthony C. Lapuz

Prof. Kari Ann V. Bitgue

Dr. Victoria T. Lupase

Dr. Pamela R. Castrillo

Dr. Lucila T. Lupo

Dr. Felix C. Chavez, Jr.

Dr. Norlan B. Martinez

Prof. Apolinar Henry Fernandez

Prof. Maricar Gay V. Panda

Dr. Bonifacio G. Gabales, Jr.

Prof. Genevieve A. Pilongo

Prof. Luzviminda L. Gonzales

Dr. Elsie A. Tee

Prof. Melodie Claire W. Juico

Dr. Macario D. Tiu

 

International Reviewers

Dr. Celine Arat-Cabading

University of Guam

Guam, USA

 

Dr. Stamatis Kalogerakos

Cranfield University

United Kingdom

 

Dr. Djuwari Djuwari

STIE Perbanas

Indonisia

 

Dr. Ana Joy P. Mendez

University of Guam

Guam, USA

 

Dr. Kathryn Holmes

University of Newcastle

Australia

 

Dr. Alejandro F. Tongco

Oklahoma State University

USA
About the Cover
Authors: Limjuco Renan P.
Published: February 2016

Thinking is the source of it all. Great ideas start as surges of creativity and intellect. As portrayed in the cover, the various fields of study are revealed like flashes of thumbnails that hover around the “brain.” Just like in one’s pursuit of research, the simple idea must be allowed to evolve amidst all the challenges that confront it. Research has a life of its own. It is destined to be exposed.
About the Monogram
Authors: Limjuco Renan P.
Published: February 2016

The UIC (University of the Immaculate Conception) Research Journal International Edition monogram depicts two feathers used in ancient quill pens. Intentionally juxtaposed to resemble the iconic Yin Yang symbol, this abstract form signifies the physicality and spirituality of the highest form of intellectual activity—research. Aside from capturing the dramatic glimpse of ancient writing, which is the essential aspect of any form of publication, this powerful image also depicts the interconnectivity and balance between the multidisciplinary and international characters of this research publication. In response to the UIC quest for quality research outputs that can pass international peer review process, this symbol was conceptualized to remind all researchers of UIC to commit to the truthfulness, credibility, and validity of information derived from the rigors of research writing. Created by Jo Caliph G. Rivera, this monogram is meant to become a unique emblem in every cover of UIC Research Journal International Edition.

 

Dr. Renan P. Limjuco

Editor in Chief

UIC Research Journal

International Edition
Consolidating Google APIs for Improved Project Management
Authors: Barrios Exander T. , Gido Helenna Jo R. , Palarca Jenny Mae S. , Villegas Geraldine E.
Published: February 2016

The primary purpose of the study was to consolidate Google APIs to improve project management. The applications used for the development were Google Docs, Google Calendar, and Google Talk. The GDocs4Ruby was used to interact with the Google documents API and to store everything in the Google server. The researchers used classes that were included in the GDocs4Ruby library and the GCal4Ruby was used to interact with the Google Calendar API. GData4Ruby was applied to interact with any Google APIs based on the GDATA protocol. Through GData4Ruby, the researchers had the ability to authenticate with Google and had an access rule class for managing user through Google Access Control List (ACL) feeds. Constructive research method was applied. Interviews and survey were utilized to facilitate the data gathering and evaluation of the study. It was found that six out of seven or 85.71% of the testers were able to achieve the functionalities mentioned during the User Acceptance Testing (UAT) conducted to experts in project management.
Quality and Safety Evaluation of Commercial Mangosteen (Garcinia mangostana Linn.) Capsule
Authors: San Juan Ma. Eva C. , Alagos Razle R. , Alocelja Ruiza R. , Manluyang Jr. Ruel S.
Published: February 2016

Many herbal food supplements today are being sold in market and most popular among them is the commercial mangosteen (Garcinia mangostana Linn.) capsule. With the present scenario, this study focused on ensuring the quality of commercial mangosteen (Garcinia mangostana Linn.) capsule being sold in the market for consumer’s safety. Based on results of experimentations, the sample of commercial mangosteen capsule passed all the tests conducted. The disintegration time using water and simulated gastric fluid as a solvent was within the 30 minutes specification set by 22nd edition of USP XXII/ NF XVII, which was at 21.88 minutes and 10.83 minutes, respectively. Result also revealed that commercial mangosteen capsule was more soluble in simulated gastric fluid when compared to water since, in the first test, all the capsule disintegrated completely within 30 minutes at a lower mean disintegration time and the residue left after the experimentation was lesser. For microbial limit tests, the Aerobic Plate Count (CFU/g) was 540 (normal limit - 104), Yeast (CFU/g) at 135 (normal limit -103), and Molds (CFU/g) at 10 (normal limit- 103). Lastly, for heavy metal load, Cadmium (ug/g) has <0.05 (normal limit - 0.3) and Lead (ug/g) has 2.73 (normal limit – 10). All results conformed to FDA Guidelines. Therefore, commercial mangosteen capsule has met the minimum quality control standard and is safe for human consumption based on the 22nd edition of USP XXII/NF XVII specifications and FDA Guidelines.
Toxicological and Antimicrobial Evaluations of Formulated Ointment from Eskwater (Mikania cordata Asteraceae) Leaf Extract against Trichophyton mentagrophytes and Methicillin-Resistant Staphylococcus aureus
Authors: Nogodula Judee N. , Ducut Lady Ron M. , Edorot Jelah Mae F. , Egagamao Aizovelle T.
Published: February 2016

Eskwater (Mikania cordata) is a medicinal plant in treating wounds by B’laan tribe. It is a useful treatment in the emerging cases of difficult-totreat infections caused by Methicillin-Resistant Staphylococcus aureus and Trichophyton mentagrophytes. To validate such claims, this study was conducted to assess the toxicological and antimicrobial properties of eskwater leaf extract through the acute dermal toxicity, antibacterial activity, Minimum Inhibitory Concentration (MIC), potency test with the standard drug and mutagenicity assay. Toxicity level was attained using female albino rats with the dose of 1000 mg/kg (Sighting study) while 1000 and 2000 mg/kg for Main study. Kirby-Bauer Method was employed for antibacterial activity using Vancomycin and Terbinafine as positive controls. MIC was determined in two-fold serial dilution and Ames test was employed for mutagenic assay using Salmonella typhimurium TA98. Hydrophilic base with low, medium and high doses were incorporated, respectively for ointment formulation. Results showed that eskwater extract was not toxic based from OECD 404 guideline and had partially active action against MRSA ATCC 43300 (13.66 ± 0.5778) and showed no inhibition in fungal growth. MIC was effective at 0.05 g/ml against MRSA only. It exhibited comparable potency with Vancomycin at the concentration of 0.32 mg and lastly, no noticeable mutagenic property was established. One-Way ANOVA and Post Hoc Tukey’s multiple comparison tests were employed in the efficacy of ointment. A significant difference resulted among the formulated ointment and positive control indicating that the three doses of formulated ointment and positive control have different potential to induce inhibition on the growth of MRSA. Ointment evaluated on its physical characteristics appeared greenish brown, has pH 6, good spreadability and washes out easily.
Bacteriological, Toxicological and Mutagenic Evaluations of the Formulated Ointment of Knobweed (Hyptis capitata Jacq.) Leaf Extract against Methicillin-Resistant Staphylococcus aureus
Authors: Nogodula Judee N. , Mancenido Rose Ann S. , Miranda Ivana Lee M. , Quiñon Darlene S.
Published: February 2016

The emergence of multiple drug resistant Methicillin-Resistant Staphylococcus aureus (MRSA) has become a global problem. Fortunately, many plants found from Mindanaon lumads have potential curative property for different ailments. Hence, the ethnopharmacologic practice of the knobweed (Hyptis capitata) was scientifically assessed for antibacterial activity, toxicity, mutagenicity and pharmacological screenings of crude leaf extract in formulation of ointment as treatment of MRSA infections. Acute Dermal Toxicity employed female albino rabbits for 14 days. Antibacterial screenings used Kirby Bauer, Minimum Inhibitory Concentration (MIC) and potency tests. Ames test used Salmonella typhimurium TA98. Pharmacological assay was limited to physical characteristics of ointment and sensitivity (patch) test using albino rabbits (24 to 72 hours of observation). Results revealed that leaf extract was not toxic and had promising antibacterial action with 19.22 mm (±0.82) zone of inhibition on MRSA ATCC 43300 and 17.41 mm ±0.77 on clinical isolate. It had similar potency with Vancomycin at 6.54ug. MIC established the reduction of cell viability at 0.0263g/mL without revertant colonies grown; hence, it was not mutagenic. Efficacy of ointment with a hydrophilic base exhibited 16.11 mm (±1.73) zone of inhibition on MRSA ATCC and 16.12 mm (± 2.08) for clinical isolate. Neither erythema nor edema was observed.
Preliminary Acute Toxicological Analysis and Profiling of a Commercially Available Mangosteen (Garcinia mangostana) Xanthone Capsule
Authors: Merin Kenny James
Published: February 2016

Mangosteen is known for its wide variety of healing properties, but studies on its toxicity remained insufficient. Toxicological profile of commercially available Garcinia mangostana xanthone capsule was evaluated using a true experimental research design. Acute oral toxicity and toxicant category were employed. After the test animal was administered with xanthone capsule, biomarker such as Alanine Transaminase (ALT), Serum Glutamic Pyruvate Transaminase (SGPT), Blood Urea Nitrogen (BUN) and Serum Creatinine levels were evaluated using the Swiss albino mice. Histopathological (liver and kidney) analyses were conducted and determination of mutagenicity and heavy metals (Cd, Pb and Hg) were also pursued. Results reveal that acute oral toxicity dose had zero deaths from 300, 2000 up to 5000 mg/ kg. This classified in category 5 as nontoxic (OECD 423). ALT/SGPT value of 125.00±21.9317 was higher than the normal (0-41 u/L). BUN (7.75 ± 0.1858) and serum creatinine (93.00±20.0749) exhibited higher than the normal at 1.78-6.07 umol/L and 44 umol/L, respectively. This means the sample may cause hepatotoxicity and nephrotoxicity. Analysis on positive likelihood ratio of 1.333 was higher than the negative ratio of 0.6667 while kidney had 1.333 higher than the negative ratio of 0.6667. No revertant colony was observed using Salmonella typhimurium TA98 compared to the benzene (368 colonies). It was within the allowable limits for lead (0.003 µg/g) and mercury (0.002 µg/g). However, cadmium exceeded the limits of 0.01 having a mean of 0.23 µg/g.

 

Keywords: Pharmacology, toxicology, Garcinia mangostana, xanthone, experimental, Davao City, Philippines.
***
Authors: Limjuco Renan P.
Published: February 2016

UIC RESEARCH JOURNAL

International Edition

Volume 20, Number 2, October 2014

 
Motivational Drives and School Effectiveness Efforts of Secondary School Academicians and their Attitudinal Level towards K to 12 Implementation
Authors: Sagayno Fortunato B. , Limjuco Renan P.
Published: February 2016

Republic Act 10533 known as the Enhanced Basic Education Curriculum of the Philippines is the newest paradigm of education of the country, the implementation of which needs to be evaluated specifically on the guidelines and the attitudinal level of academicians towards it. The primary purpose of this study was to determine the motivational drives, school effectiveness efforts, and the attitudinal level of secondary school academicians of the Department of Education Davao Region XI. Descriptive correlation was utilized in this study. Sets of survey questionnaires were used to gather data from 563 academicians. The findings revealed that motivational drive and school effectiveness efforts were both rated high descriptive equivalent while the attitudinal level had obtained high positive descriptive equivalent which means that academicians experienced great extent of positive attitude towards K to 12 implementation. Based on the data gathered, there is a significant relationship between motivational drives, school effectiveness efforts and attitudinal level towards K to 12 implementation. While motivational drive and school effectiveness efforts significantly influenced the attitudinal level of the academicians towards the implementation of K to 12, the school effectiveness efforts among secondary school academicians had a greatest significant influence among other variables which has positive correlation, showing that school academicians’ primary concern was on the effectiveness in their field of work on the aspect of implementation.
 

 

Keywords: 
Motivational drives, school effectiveness, K to 12, Philippine education, descriptive correlation, Davao City, Philippines
Davao Doctors College Tracer Study of Bachelor Of Science In Nursing
Authors: Pia Mary Ann C. , Matunding Leonardo C. , Salvador Gina C.
Published: February 2016

Tracer study is an endeavor to survey the performance of graduates in the job market upon completion of study in the institution. The study aimed to obtain data on the status of the graduates in terms of employability, job status, type of employment, monthly income and employer’s information. It aimed to gather information on the graduates’ perspective on the academic program, facilities and the impact of teaching and learning process experienced in the institution. It utilized a total of 370 of the 4,198 BSN graduates of Batch 2003 to Batch 2009 as respondents. Descriptive method was used in the study. In terms of the educational profile, results showed that most of them are registered nurses, most of whom had not taken any other examinations. Further, most of them had attended only local trainings and seminars. For the employment profile, the results revealed that most of the respondents were employed with regular status and occupying staff nurse position. After graduation, most of the respondents worked in a nursing–related job. As to the graduates’ perspective on the academic program, facilities and the impact of teaching and learning process experienced in the institution, most of them cited skills in communication, human relation, critical thinking and problemsolving as the ones they developed which also helped them procure the job.
 
Keywords:Nursing, tracer study, descriptive, Davao Doctors College, Philippines
Uji Sifat Fisik Ransum Ayam Broiler Bentuk Pellet yang Ditambahkan Perekat Onggok Melalui Proses Penyemprotan Air
Authors: Retnani Yuli , Hasanah Nining , Rahmayeni Rahmayeni , Herawati Lidy
Published: February 2016

The physical characteristic test of broiler ration pelleted that added of onggok as binder with water spraying processABSTRACT. Pellet is a kind of solidified and mechanically compressed feed. Problem frequently rise is that pellet shape is easily damaged, brittle, and broken during production, transportation and storage processing. The purpose this study was to know the influence of onggok addition as binding agent with 5% water spraying on the physical characteristic of pellet form ration.The data were analyzed by analyzed of variance and the significant experiment results would be examined by orthogonal contrast test. The treatments consist from the first experiment were: A1 = basal ration + 0% spraying water, A2 = basal ration + 5% spraying water, A3 = basal ration + 10% spraying water and A4 = basal ration + 15% spraying water. The best result from the first experiment would be used for the second experiment, the treatments second experiment were: R1 = basal ration + 5% spraying water + 0% onggok, R2 = basal ration + 5% spraying water + 2% onggok, R3 = basal ration + 5% spraying water + 4% onggok and R4 = basal ration + 5% spraying water + 6% onggok.The parameters were water content, water activity, specific gravity, loose bulk density, compressed bulk density, angle of repose, modulus of fineness, average particle sizes and the durability of pellet form. The conclusion of the experiment that added of onggok as binder significant influenced of the characteristic physical pellet, i.e.: increased loose bulk density, compressed bulk density, modulus of fineness, average particle sizes, and durability of pellet, but decreased specific gravity, water content, angle of repose and water activity.
Aktivitas Antimikroba Khamir Asal Dadih (susu kerbau fermentasi) Terhadap Beberapa Bakteri Patogen
Authors: Yurliasni Yurliasni
Published: February 2016

Antimicroba activity of yeast dadih (buffalo milk fermentation) origin on pathogenic bacteriaABSTRACT. Research was carried out to study the effect of antimicrobial activity of yeast found in dadih. Yeasts chosen were isolated from dadih (traditionally buffalo milk fermentation). The objective is to know the ability of yeast to inhibit the growth of pathogenic bacteria in milk. They were Candida curiosa, Brettanomyces custersii, and Kluyveromices lactis. Kluy. lactis has strong antimicrobial on Bacillus subtilis with zone of inhibition ± 5mm, C. curiosa on E.coli (5mm) and C. curiosa and Brett. custersii also have strong antimicrobial on S. aureus consecutively 5,75 and 7 mm. As a conclusion the result showed that all of three yeasts selected have capability to stop the growth of pathogenic bacteria of buffalo milk origin in five days.
Pola Usaha Peternakan Kambing dan Kinerja Produktivitasnya di Wilayah Eks-Karesidenen Banyumas Jawa-Tengah
Authors: Sodiq Akhmad
Published: February 2016

Goat farming pattern and their productivity in the area of eks-karesidenan banyumas, central javaABSTRACT. Goats play an important role in the livelihood of rural people in upland and lowland farming systems in the areas of Eks-Karesidenan Banyumas Central Java. The main focus of this study presented in this paper, consist of (i) documenting the regional goat farming pattern, and (ii) find out the level of goat productivity in their farming. Importance of this study related to the development strategic fof their goat farming. Study was conducted in the areas of Eks-Karesidenan Banyumas Central Java (consist of Banyumas, Purbalingga, Banjarnegara, and Cilacap regencies). Upland and lowland areas were selected by purposive sampling method. Qualitative and quantitative descriptive statistic was applied in this study. Most of goats are raised within traditional system, characterized by small-scale production. The production in upland and lowland areas focused on single purposes for producing kid goats from Peranakan Etawah (PE) and Jawa Randu (JR) breeds. PE goats focus on dual purposes for producing milk and meat are mostly found on upland. Flock size of PE and JR goats in upland and lowland ranges from 2 to 9 head (mean: 3.8 head) and from 1 to 6 head (mean: 2.7 head), respectively. Flock size of PE goat focus on dual purposes ranges from 8 to 75 head. PE and JR goats in upland and lowland were dominated by double litter, followed by single and triplets. The highest litter size (1.89 kids) was found in lowland, followed by single purpose of PE and JR goats in upland (1.78 kids), and dual purposes PE goats in upland areas (1.66 kids). Pre-weaning mortality was highest (9.5%) in lowland areas for single purpose of PE and JR goats. Does reproduction and productivity ranges from 1.76-5.24 kids/does/year and 12.92-87.42 kg/does/year, respectively. Doe productivity was lowest (12.92 kg/does/year) in single purpose of PE and JR at lowland due to low of their survival rate and weaning weight.
Pertambahan Bobot Badan dan Waktu Pembusukan Daging Ayam Broiler yang Diberi Ekstrak Jaloh Dikombinasi dengan Kromium
Authors: Sugito Sugito , Rahmi Erdiansyah , Azhari Azhari , Isa M.
Published: February 2016

The gain body weight and Spoilage of meat broiler which giving jaloh extract to combine with chromiumABSTRACT. A study was conducted on broiler chickens under heat stress to evaluate the effect of combined jaloh (Salix tetrasperma Roxb) extract with chromium mineral in the body weight, value of feed ratio conversion, and time to meat to become decomposed. Twenty four of 20-day old Cobb female broiler chickens were randomly assigned and divided to 4 treatment groups. Completely randomized design was used in this study. The treatments were as follows: 1) chickens given no heat stress and jaloh extract nor chromium (tCp); 2) chickens under heat stress without given jaloh extract nor chromium (Cp); 3) chickens under heat stress and given jaloh extract in 1.000 mg/lt drinking water (Cp+Ej); and 4) chickens under heat stress, given jaloh extract in 1.000 mg/lt drinking water, and chromium in 1.000 ug/lt drinking water (Cp+Ej+Cr). Heat stress given was 33 ± 1oC of cage temperature during 4 hours per day within 15 days. Jaloh extract and chromium treatments in drinking water were given at 2 hour before cage temperature reaching 33 ± 1oC and were stopped being given after 1 hour, when cage temperature back to room temperature. On the 16th day of the study chickens were measured before slaughtered. Samples obtained were chicken meat that taken from breast part (musculus pectoralis). The result suggested that either giving jaloh extract per se at the dose of 1.000 mg/lt in drinking water or combine it with chromium that given two hours before cage temperature reaching 33 ± 1oC, can prevent chickens from decreasing their body weight, decreasing value of feed ratio convertion, and extending time to meat to become decomposed.
Kualitas Karkas Domba Lokal yang Diberi Pakan Jerami Padi Fermentasi dengan Suplementasi Minyak Kedelai
Authors: Patriani Peni , Sumarmono Juni , Suryapratama Wardhana
Published: February 2016

Carcass quality of local sheep fed fermented rice straws supplemented with soybean oilABSTRACT. The aim of this study was to determine if supplementation of soybean oil in the ration in order to increase conjugated linoleic acid of meat has concurrent effects on carcass characteristics and qualities of thin-tailed sheep. Sheep were fed fermented ricestraws supplemented with 0, 3 and 6% soybean oil. The experimental design used was a Randomized Completely Block Design. Eighteen sheep with initial age of 6-8 months and initial bodyweight of 17.46 ± 1.61 kg were used. The treatments consisted of soybean oil supplementation 0, 3 and 6%. Variables measured were carcass conformation, GR thickness, backfat thickness, subcutaneous fat score, and kidney and pelvic fat score. Result showed that sheep fed ration with 0 % soybean oil produced carcass with carcass conformation score of 8.50 ± 1.05, GR thickness 11.50 ± 1.05 mm, back fat thickness 2.67 ± 0.82 mm, subcutaneus fat score 3.50 ± 0.55 mm, kidney and pelvic fat score 2.00 ± 0.00. Sheep feed ration with 3% soybean oil; carcass conformation score 8.67 ± 1.21, GR thickness 11.67 ± 1.21 mm, back fat thickness 2.83 ± 0.98 mm, subcutaneus fat score 3.50 ± 0.55, kidney and pelvic fat score 1.83 ± 0.4. Sheep feed ration with 6% soybean oil; carcass conformation score 9.00 ± 1.26, GR thickness 12.50 ± 1.52 mm, back fat thickness 3.33 ± 0.82 mm, subcutaneus fat score 3.50 ± 0.55, kidney and pelvic fat score 1.83 ± 0.41. Score of carcass conformation, thickness of GR, thickness of back fat, score of subcutaneus fat, and score of kidney-pelvic fat were not influenced by supplementation of soybean oil. In conclusion, suplementation soybean oil in the ration to increase conjugetaed linoleic acid has no significant concurrent effect on carcass quality characteristics of local sheep.
Pengaruh Taraf Penyemprotan Air dan Lama Penyimpanan Terhadap Daya Tahan Ransum Broiler Finisher Berbentuk Pellet
Authors: Retnani Yuli , Putra Edo Duanda , Herawati Lidy
Published: February 2016

The effect of different water spraying level and storage period on endurance of pellet broiler finisherABSTRACT. The storage is very important in a farm management, because this activity can take care of feed stability which enough and preferable to be consumed by animal and also strive for animal food availability continuously. The purpose of this research is to know the water spraying level and variation of storage period against the endurance of pellet broiler finisher ration. This research was designed by a Completely Randomized Design Factorial with two factors (water spraying different level and storage period) with four replicates, the significant result were analyzed by orthogonal contras test. On this experiment, the different level of water spraying (0, 3, 6%) were conducted during mixing at conditioning process, and then the ration were stored until four weeks to study the endurance of pellet e.g.: water content (%), water activity, specific gravity (ton/m3), pellet durability index (%), attack of insect. The water content, specific gravity, and pellet durability index was very significantly (P0.01) reduce by water spraying different level, and not significantly on water activity. The storage period showed very significantly (P0.01) increase the specific gravity, pellet durability index, and the insect attacks, and significantly (P0.01) decreased the water activity, but not effect on water content. Interaction among spraying level and storage period is very significantly (P0.01) on the values of water content, water activity, specific gravity and pellet durability.
Analisis Keragaman Gen Laktoferin Pada Sapi Friesian-Holstein Dengan Metode Pcr-Rflp
Authors: Mariana Elmy
Published: February 2016

Polymorphism analysis of lactoferrin gene on holstein-friesian cow with PCR-RFLP methodABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene in Holstein-Friesian (HF) cows. The study was conducted on 281 heads of HF lactating cows coming from dairy farmers in Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65.5%) and AB (34.5%) genotypes.
Analisis Kawasan Usaha Pengembangbiakan dan Penggemukan Sapi Potong Berbasis Sumberdaya Lokal Pedesaan untuk Program Nasional Percepatan Pencapaian Swasembada Daging Sapi
Authors: Sodiq Akhmad
Published: February 2016

Analysis of livestock estate of village breeding centre (VBC) and village fattening centre (VFC) for beef cattle based on local resources to support national program of cattle meat self-sufficiencyABSTRACT. The main focus of this study were (1) documenting the profile of livestock estate of Village Breeding Centre (VBC) and Village Fattening Centre (VFC) for beef cattle under local resources base, (2) documenting the profile of local resources in terms of the potential of individual farmer and group dynamic, (3) documenting the degree of livestock estate consist of: elementary, middle and advance. Livestock On-Farm Trials was conducted by survey on Village Breeding Centre and Village Fattening Centre. 81 VBC and VFC located at 5 regencies in Central Java (Cilacap, Banyumas, Purbalingga, Banjarnegara, and Kebumen) were involved in this study. Qualitative and quantitative descriptive statistics were applied in this study. Conclusions remark of this study: (1) The profile of livestock estate for breeding were dominated by secondary grade (92%), followed by elementary (4%) and advance (4%) grades. The profile of livestock estate for fattening better than breeding estate, the grade of livestock estate for breeding was advance (18%) and secondary (82%) and no elementary grade.; (2) The potencial of individual farmer under midle grade (77%) and high grade (23%). These good conditions are really support for developing breeding and fattening beef calte estate; (3) The poteny of group farmer institution comprise of the potency of social resources and group dynamic. The potency of social resources was dominated (85%) by high grade, followed by midle grade (15%). The good condition of social resources potency are really support livestock esate development, unfortunalty this condition unsupported by group dynamic where they are tend to be statis and decline (76%); (4) Based on the development process, beef cattle estate consists of three categories: elementary, secondary, and advance degree. Only 4% of elementary degree was found at beef cattle breeding. Mostly, they are under condition of secondary grade (92% at breeding centre, and 82% at fattening centre). The advance grade was found in small number (at breeding and fattening centre were 4 and 18%, respectively).
Analisa Kualitas Susu Kambing Peranakan Etawah yang Disterilkan pada Suhu dan Waktu yang Berbeda
Authors: Zakaria Yusdar , Yahya Helmi M. , Safara Yuda
Published: February 2016

Analyze of Etawa’s milk goat quality sterilized at the Different Temperature and TimeABSTRACT. The objective of this experiment is to study the quality of sterilization of etawa’s goat milk at different temperature (110oC and 120oC) and different time (3 second and 6 second). The quality of sterilization of etawa’s goat milk was evaluated by measuring crude protein, crude fat, pH value and amount of microorganism. The study used a factorial completely randomizes design. The result showed that the different temperature and time of sterilized goat milk have not significant (P0,05) on crude protein, crude fat, pH value and amount of microorganism. The result also showed that no interaction between temperature and time. The conclusion of the research indicated that all the experiment have the same quality.
Kontribusi Ekonomi Perempuan Penjual Ayam Goreng Gerobak di Beberapa Pasar Satelit di Kota Padang
Authors: Yuzaria Dwi , Fitrini Fitrini
Published: February 2016

Economical contribution of women in merchant-chart-fried chicken business in satellite market in padang cityABSTRACT. As social-economic demands increased, the complexity of family needs grew higher. To fulfill them, woman has to take part in making a living. This research aims to see economical performance of women in running merchant-cart-fried-chicken, identifying the obstacles faced and giving alternatives of solution. This research was conducted by survey method, with respondents of merchant-cart-fried-chicken seller in several markets in Padang city. Sample was taken with proportional allocation of 15 people. Data was analyzed by descriptive analysis method. The research showed that economical performance of women merchant-cart-fried-chicken seller is low with working hour o5 5.07 hours/day. Percentage of women that take part in all activities start from planning to marketing is 32.5%. This is a potential business to develop, with average income 92.259,58 IDR/day and average R/C ratio 1.29. Internal constraints were family relation pattern that scoped women’s part in fulfilling family need, meanwhile the external constraints were the difficulties in acquiring more capital to develop the business and the price fluctuation of chicken as a main raw material. Alternative solution that can be offered was opening the business at home so that women can spare more time to work the business and accessing soft loan with light requirements. The conclusion of the research was that women took part in making a living for the family, however the contribution is low due to patriarchy value as an internal constraint and capital problem as external constraint. The solution offered was taking soft loan and building relation with raw material supplier.
Populasi Ascaridia galli Dalam Usus Halus Ayam Yang Diberikan Kombinasi Ekskretori/Sekretori L3 dan Imunoglobulin Yolk
Authors: Darmawi Darmawi , Balqis Ummu , Tiuria Risa
Published: February 2016

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A – F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.
Motilitas Spermatozoa Ayam Kampung dalam Pengencer Air Kelapa, NaCl Fisiologis dan Air Kelapa-NaCl Fisiologis pada 25-29°C
Authors: Lubis Triva Murtina
Published: February 2016

Spermatozoa motility of local chicken in coconut water, physiological nacl and physiological nacl-coconut water at 25-29°c)ABSTRACT. This study aimed to determine the effect of coconut water diluent, physiological NaCl and combination of coconut water-physiological NaCl to the survival of chicken spermatozoa at room temperature (25-29°C). Semen was collected from a local chicken of 1.5 years old with weight of 2 kg in massage twice a week. Once evaluated, a good quality semen was divided into 3 treatment groups: coconut water (P1), physiological saline (P2), and its combination (P3) with the addition of 20% egg yolk [(P1) 80:0:20 (P2) 0:80:20 and (P3) 40:40:20] and stored at room temperature. Evaluation of spermatozoa motility conducted every 1 hour until reaching 40% of spermatozoa motility. Data obtained were analyzed using analysis of variance (ANOVA) with design patterns Split-plot and if there are differences between treatments tested by Duncan's multiple test. The results showed that motility of spermatozoa is significantly different (p 0.05) between storage time and diluent and there are interactions between storage time and diluent. P2 is able to maintain the minimum percentage of inseminated sperm motility (40%) for 4 hours, followed by P3 for 1 hour, and the lowest percentage motility of spermatozoa present in P1 for 0 hours. It can be concluded that the percentage of spermatozoa motility in group P2 is higher than P1 and P3.
Hubungan Polimorfisme Gen Laktoferin dengan Kualitas Susu pada Sapi Perah Friesian-Holstein
Authors: Mariana Elmy
Published: February 2016

Association of Lactoferrin Polymhorphism on Milk Yield and Milk Quality in Holstein-Friesian Dairy CowsABSTRACT. The purposes of this study were to identify the polymorphism of the lactoferrin gene and the association of genetic variants of the lactoferrin gene on milk quality in Holstein-Friesian (HF) cows. The study was conducted on 194 heads of HF lactating cows coming from semi intensive of small dairy farmers in two villages of the KPSBU Lembang, Lembang district. Investigation on variant genotypes of the lactoferrin gene used PCR-RFLP method. Genotyping of the lactoferrin gene with EcoRI restriction enzyme produced two genotypes, i.e. AA (65%) and AB (35%) genotypes. Both the AA and AB genotypes had inconsistency in controlling component of the milk quality. Further, the AB lactoferrin cows had a higher frequency in the classification of low somatic cells count (0.75), while the AA cows were at a higher frequency in the classification of high somatic cells count (0.60). It was concluded that there is no strong relationship between intron 6 lactoferrin gene with milk quality parameters. Further, the AB genotype of the lactoferrin gene had a consistency in producing lower somatic cells count compared to the AA genotype.
Pengaruh Level Subtitusi Protein Sel Tunggal (Cj Prosin) Pada Pakan Komersial Terhadap Performan Ayam Broiler
Authors: Samadi Samadi , Delima Mira , Hanum Zuraida , Akmal Muhammad
Published: February 2016

The effect of level substitution of single cell protein (CJ Prosin) by commercial feed on broiler performanceABSTRACT. A research to find out the effect of the substitution level of single cell protein in commercial feed on Broiler’s performance was conducted from June to December 2011. The purpose of this experiment is to find out alternative protein sources for animal feedstuff that can be used as protein substitution in animal feed on body weight, feed consumption and feed conversion efficiency. The experiment is considered as preliminary experiment to observe the response of single cell protein on broiler performance. This research was carried out at experimental farm, Animal Husbandry Department, Syiah Kuala University. Totally, 250 DOC of Cobb breed with the average body weight 36,78±2,32 gr. used in this experiment. Completely Randomized Design was applied in this experiment with 5 treatments; P0 (commercial feed), P1(commercial feed substituted with 6% SCP), P2 (commercial feed substituted with 12% SCP) dan P3 (commercial feed substituted with 18%CSP). Each treatment consist of 5 replications with total of 20 treatment’s units. Feed was formulated with isoprotein and isoenergy based on NRC requirement ( 23% protein ; 3200 kcal ME/kg at the age of 1-2 week and 20 % protein ; 3200 kcal ME/kg at the age of 4-7 week). Data was analyzed by using SPSS program SPSS (Window 2008). Differences between treatment were stated by Duncan’s multiple range test. The results of the experiment showed that commercial feed substituted with the level of SCP was not significantly difference (P0.05) on body weight gain, feed consumption, and feed conversion ratio. On the other hand, administration of SCP as substituted feed commercial on the level of 12 % and 16 % influenced significantly (P0.05) on body weight gain and feed conversion ratio, but not on feed consumption at the level of 12% SCP. As a conclusion, the use of SCP as animal feed sources in commercial feed only could be tolerated at the level of 6%.
Deteksi Antibodi Serum Terhadap Virus Avian influenza pada Ayam Buras
Authors: Darmawi Darmawi , Manaf Zakiyah Heryawati , Darniati Darniati , Fakhrurrazi Fakhrurrazi , Abrar Mahdi , Erina Erina
Published: February 2016

Detection on Serum Antibodies of Native Chickens to Avian influenza VirusABSTRACT.  An important approach of controlling against Avian Influenza should be determined to detect the antibody titres of bird flu caused by Influenza virus H5N1 in Indonesia. The aim of the present study was to detect the antibodies to Avian Influenza in serum of native chickens. This study utilized 123 serum samples collected from the axilaris vein (left or right) of native chickens. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that indication of natural infection by Avian Influenza (H5N1) in native chickens, as shown that out of 123 serum samples, 16 (13,01%) were tested positive by HI, while only 10 (8,13%) were tested protective to Avian influenza infection. Based on the results we obtained, a conclusion that natural infection by Avian influenza virus stimulated variety level of formation antibody titres in native chickens.
Produktivitas Sapi Potong pada Kelompok Tani Ternak di Pedesaan
Authors: Sodiq Akhmad , Budiono Machfudin
Published: February 2016

Beef Cattle Productivity under Group of Farmer at the VillageABSTRACT. Development of beef cattle in Indonesia should be carried out through sustainable production system approach especially under smallholders in the village areas. The information of beef cattle productivity level under current production system is very important for the basis data in relation to the development strategic program. The purpose of current study was to find out the productivity of beef cattle production under group of farmer at the village areas. Survey method at 34 farmer groups of beef cattle located at Cilacap, Banyumas, Purbalingga, Banjarnegara and Kebumen regencies of Central Java was implemented. The beef catle productivity in terms of cow-calf operation and fattening were recorded. Data analysis by qualitative and quantitative descriptive statistics. This study found that Ongole Cross, Sumba Ongole Cross, Simental Cross and Charolois Cross were raised for fattening and Brahman Cross for cow-calf operation. The performance of beef cattle of fattening purposes was moderate condition. Body Condition Score (BCS) ranged from 4 to 6 (scale 1-9) with the modus 4 (for Ongole Cross, Sumba Ongole Cross) and 5 (for Simental Cross and Charolois Cross). The productivity of cow-calf operation was very low with the reproductive rate and preweaning calf mortality were 6 and 25 percent, respectively. Good Farming Practice with attention on selection of breeds and strengthens feeding aspects could be taking into account in order to improve beef cattle productivity under group of farmer at the village areas.
Biotransformasi Limbah Ikan Menjadi Bahan Pakan untuk Ruminansia
Authors: Rimbawanto Efka Aris , Suwandyastuti S.N.O. , Rahayu Sri
Published: February 2016

Biotransformation of fish waste as feed matter for ruminantABSTRACT. Fish waste (head, viscera and tails) which ensilage with saccharomyces sp and lactobacillus sp, had a high quality and protein content of about 53,18 ± 0,83% and a good aroma. An experiment have been conducted by experimental method with in sacco trial and in vivo trial. The treatment tested were 4 kind of protein source : (1) fish waste silages mixed with tapioca ; (2) fish waste silages mixed with rice bran ; (3) soy bean kernels and (4) fish meal. The in sacco trial was carried out on 3 fistulated cows, to study the rate of protein degradability in the rumen. The in vivo trial was carried out by Completely Randomized Design on 16 heads of fat tail rams. The variable measured were: rumen fermentation product (VFA and N-NH3); energy and protein digestibility; daily body weight gain of the rams. The experiment showed, that the source of protein use have no significantly effect upon VFA both on the VFA total and individual VFA (acetate, propionate and butyrate). The highest propionate reach by soya bean kernels (P 0.01). Based on the all variables measured, it was indicated the silage of fish waste can be used to substituted of soya bean kernels or fish meal in the ration of sheep.