bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards Structural and Aeroelastic Similarity in Scaled Wing Models: Development of an Aeroelastic Optimization Framework
Authors: Filippou Evangelos , Kilimtzidis Spyridon , Kotzakolios Athanasios , Kostopoulos Vassilis
Published: February 2024

The pursuit of more efficient transport has led engineers to develop a wide variety of aircraft configurations with the aim of reducing fuel consumption and emissions. However, these innovative designs introduce significant aeroelastic couplings that can potentially lead to structural failure. Consequently, aeroelastic analysis and optimization have become an integral part of modern aircraft design. In addition, aeroelastic testing of scaled models is a critical phase in aircraft development, requiring the accurate prediction of aeroelastic behavior during scaled model construction to reduce costs and mitigate the risks associated with full-scale flight testing. Achieving a high degree of similarity between the stiffness, mass distribution and flow field characteristics of scaled models and their full-scale counterparts is of paramount importance. However, achieving similarity is not always straightforward due to the variety of configurations of modern lightweight aircraft, as identical geometry cannot always be directly scaled down. This configuration diversity has a direct impact on the aeroelastic response, necessitating the use of computational aeroelasticity tools and optimization algorithms. This paper presents the development of an aeroelastic scaling framework using multidisciplinary optimization. Specifically, a parametric Finite Element Model (FEM) of the wing is created, incorporating the parameterization of both thickness and geometry, primarily using shell elements. Aerodynamic loads are calculated using the Doublet Lattice Method (DLM) employing twist and camber correction factors, and aeroelastic coupling is established using infinite plate splines. The aeroelastic model is then integrated within an Ant Colony Optimization (ACO) algorithm to achieve static and dynamic similarity between the scaled model and the reference wing. A notable contribution of this work is the incorporation of internal geometry parameterization into the framework, increasing its versatility and effectiveness.
Combustion of Date Stone and Jojoba Solid Waste in a Hybrid Rocket-like Combustion Chamber
Authors: Alsaidi Saleh B. , Huh Jeongmoo , Selim Mohamed Y. E.
Published: February 2024

The performance of two solid biomass wastes, date stone and jojoba solid waste, was experimentally examined for their potential application in combustion and propulsion systems. The fuels were tested in a hybrid rocket-like combustion environment, and the test result was analyzed with combustion and propulsion parameters. The performance of both fuels was comparatively evaluated and compared with a conventional hydrocarbon fuel in a hybrid rocket, with paraffin wax serving as a baseline. A compression device was introduced to compress the solid biomass wastes into a circular-shaped fuel grain compatible with a hybrid rocket combustion chamber with a hot surface ignitor. Thermogravimetric analysis (TGA) and chemical equilibrium analysis (CEA) results revealed that the performance of the biomass fuel can be comparable to conventionally used hydrocarbon paraffin-wax-based propellant within a certain range of oxidizer-to-fuel ratio, in terms of theoretical specific impulse performance. Through experimental performance tests, it was found that the compressed biomass fuel grains were successfully ignited and produced thrust. Both biomass fuels tested in a hybrid rocket combustion chamber are expected to pave the way for further developments in biomass fuels in the waste-to-energy field for their application in combustion and propulsion systems, potentially replacing fossil fuels with renewable resources.
Characterizing Satellite Geometry via Accelerated 3D Gaussian Splatting
Authors: Nguyen Van Minh , Sandidge Emma , Mahendrakar Trupti , White Ryan T.
Published: February 2024

The accelerating deployment of spacecraft in orbit has generated interest in on-orbit servicing (OOS), inspection of spacecraft, and active debris removal (ADR). Such missions require precise rendezvous and proximity operations in the vicinity of non-cooperative, possibly unknown, resident space objects. Safety concerns with manned missions and lag times with ground-based control necessitate complete autonomy. This requires robust characterization of the target’s geometry. In this article, we present an approach for mapping geometries of satellites on orbit based on 3D Gaussian splatting that can run on computing resources available on current spaceflight hardware. We demonstrate model training and 3D rendering performance on a hardware-in-the-loop satellite mock-up under several realistic lighting and motion conditions. Our model is shown to be capable of training on-board and rendering higher quality novel views of an unknown satellite nearly 2 orders of magnitude faster than previous NeRF-based algorithms. Such on-board capabilities are critical to enable downstream machine intelligence tasks necessary for autonomous guidance, navigation, and control tasks.
The Effect of Foliar Application of Oligogalacturonides on the Functional Value of Turfgrass
Authors: Radkowski Adam , Radkowska Iwona , Kozdęba Michał , Khachatryan Karen , Wolski Karol , Bujak Henryk
Published: February 2024

Turf grasses play a crucial role in enhancing the beauty and usability of landscapes, gardens, parks, and sports facilities due to their functional and aesthetic properties. However, various unfavourable conditions, such as plant disorders and environmental pressures, can compromise their amenity value. Ongoing research aims to identify natural remedies that improve the quality and resilience of these grasses. A study was conducted at the Experimental Station of the Agricultural University of Krakow (50°07′ N, 20°05′ E) to evaluate the practical value of the turf produced by seeding of the ‘Super Lawn’ grass mixture. The experiment involved applying a spray containing oligogalacturonides at two doses: 1.0 and 2.0 dm3∙ha−1, along with a commercial fungicide. The traits were analysed using a 9-point scale. Plants in variant III (treated with the higher dose of oligogalacturonides) and variant IV (treated with the commercial fungicide) exhibited the highest aesthetic and functional values. The application of oligogalacturonides and a commercial fungicide resulted in a decrease in plant diseases. The treatment area showed a reduction in pink snow mould (Microdochium nivale) and leaf spot incidence compared to the control area. Variant II showed enhanced outcomes with the application of 1.0 dm3∙ha−1 of the preparation. In this area, the plant canopy had greater coverage, and the plants demonstrated increased resistance to pink snow mould and leaf spot compared to the plants in the control area. The use of commercial fungicide was found to be more effective than applying oligogalacturonides. Additionally, the plants that were protected with the fungicide displayed the highest values for the analysed parameters.
Design and Test of Seed–Fertilizer Replenishment Device for Wheat Seeder
Authors: Wei Liguo , Wang Qi , Niu Kang , Bai Shenghe , Wei Liang , Qiu Conghui , Han Nana
Published: February 2024

In view of existing problems, such as the seed and fertilizer supply link for wheat seeders still relying on manual installation and the lack of practical application equipment, a seed–fertilizer replenishment device based on the three-degree-of-freedom mechanical arm and screw conveying principle is designed using the seed box installation and supply as the operation scenario to replace the manual installation process. Combined with the requirements of the seed box replenishment operation, the key parameters of the replenishment robot arm and the screw conveyor auger are determined. Then, the kinematic model of the replenishment robot arm is established based on the modified D-H method, forward and inverse kinematics calculations are performed, and the workspace is analyzed using the Monte Carlo method. Based on this, the robotic arm task path is designed, the fifth-degree polynomial interpolation method is used to complete the trajectory planning, and MATLAB R2016a software is used to simulate the motion trajectories of each joint, verifying the feasibility of the trajectory planning solution. Finally, a prototype is trial-produced and quadratic regression orthogonal testing and response surface analyses are conducted to obtain the optimal working parameters of the replenishment device. The verification test shows that when the angular velocity of the lumbar joint of the replenishment device is 4°/s, the speed of screw conveyor is 90 r/min, and the angle of the big arm is 12°, the conveying loss rate is 3.98%, and the conveying efficiency is 0.833 kg/s. The relative errors with the theoretical optimal values are 4.2% and 2.4%, respectively, both less than 5%. The supply trajectory is reasonable, and the robot arm runs smoothly. This study can provide reference for the design of seed–fertilizer replenishment device for wheat seeders.
Role of an Aqueous Extract of Duckweed (Lemna minor L.) in Increasing Salt Tolerance in Olea europaea L.
Authors: Regni Luca , Tolisano Ciro , Del Buono Daniele , Priolo Dario , Proietti Primo
Published: February 2024

Salt stress is one of the preeminent abiotic stressors capable of strongly impacting crop productivity and quality. Within the array of strategies garnering interest in safeguarding crops against abiotic stresses, the use of plant biostimulants is emerging as a noteworthy avenue. For the above, there is an increasing interest in finding new plant extracts showing biostimulating effects in crops. In the present study, the efficacy of an aqueous extract from an aquatic species, the duckweed (Lemna minor L.), was assessed in olive plants (cv. Arbequina) grown in hydroponics and exposed to severe saline stress (150 mM NaCl). Salt stress caused considerable diminutions in biomass production, leaf net photosynthesis (Pn), leaf transpiration rate (E), and stomatal conductance (gs). The application of the duckweed extract resulted in a notable plant functionality recovery and counteracted the detrimental effects of the NaCl stress. Indeed, the plants stressed with NaCl and treated with the extract showed enhanced physiological and biometric traits compared to samples treated with NaCl alone. In particular, the duckweed extract improved photosynthetic activity and stomatal conductance, reduced the intercellular CO2 concentration, and ameliorated other physiological and morphological parameters. All these benefits influenced the whole plant growth, allowing samples treated with the extract to maintain a similar performance to that exhibited by the Control plants.
Eastern Gamagrass Model Simulation Parameters for Diverse Ecotypes: Leaf Area Index, Light Extinction Coefficient, and Radiation Use Efficiency
Authors: Kiniry James R. , Williams Amber S. , Jacot Jacqueline
Published: February 2024

Eastern gamagrass (Tripsacum dactyloides) is highly palatable, ideal for grazing and hay production in the United States. It is deep rooted and resilient, tolerant of flooding and drought. Objectives of this study were to develop plant parameters for different ecotypes of this grass. Data collected in field plots of diverse ecotypes included biomass, leaf area index (LAI), light extinction coefficient (k), and radiation use efficiency (RUE). Average LAI was 1.06 and average k was −1.05. The power response of k to LAI offers a new approach to simulating light interception at very low LAI values and throughout the range of LAI values of these ecotypes and similar grass species. The RUE values, which ranged from 1.16 to 4.31 g/MJ, highlight the immense diversity of eastern gamagrass. The high RUE values for the most productive ecotypes emphasizes the importance of this grass species for hay and grazing. While not as large as switchgrass (Panicum virgatum) values, it is still a prominent forage species that even is comparable to maize (Zea mays L.) in productivity when expressed as radiation use efficiency. These results are an important step in developing relationships and parameters to simulate the different ecotypes of this grass with process-based models.
Integrative Analysis of the DUF668 Gene Family in Nicotiana tabacum to Excavate Their Potential Roles in Abiotic Stress Responses
Authors: Zhang Zhenbiao , Zhang Zhongqi , Akher Sayed Abdul , Xue Jin , Wang Jie , Guo Cun , Li Zhiyuan , Guo Yongfeng
Published: February 2024

The domain of the unknown function (DUF) gene families assumes pivotal roles in plant metabolic and stress responses. However, our comprehension of the tobacco DUF668 (NtDUF668) gene family and its specific reactions to heavy metal, drought, and salt stresses remain circumscribed. In the current investigation, a comprehensive genome-wide analysis of the NtDUF668 gene family was undertaken utilizing bioinformatics tools. The results unveiled a total of 20 members in the NtDUF668 gene family, denominated NtDUF668-01 to NtDUF668-20. Phylogenetic analyses indicated a closer genetic relationship of DUF668 genes between Nicotiana tabacum and Ipomoea batatas. The examination of gene structure and conservative motifs revealed a bifurcation into two major Clades, aligning with previous studies on DUF668 gene families from various plant species, emphasizing its highly conserved evolutionary mechanism across plants. The exploration of promoter regions of NtDUF668 genes revealed a plethora of cis-acting elements associated with abiotic and biotic stresses, light signaling, and phytohormones. Gene duplication events and selection pressure analysis disclosed the segmental duplication and strong purifying selection pressure during the evolution of NtDUF668 genes. Syntenic analysis indicated a relatively conserved evolutionary mechanism of DUF668 gene families within dicotyledons. Tissue-specific expression analysis suggested that NtDUF668 family members are potentially involved in root development, floral organ formation, and abscission. The expression patterns and qRT–PCR analysis of NtDUF668 genes implied the potentially functional involvements of NtDUF668s in response to multiple abiotic stresses. Furthermore, the stress-triggered member NtDUF668-08 exhibited specific nuclear localization. In conclusion, this genome-wide analysis illuminates the composition, phylogenetic relationships, and potential roles of the NtDUF668 gene family in abiotic stress responses. The identified candidate genes, particularly NtDUF668-08, warrant further research for functional investigation.
Status of Sustainable Balance Regulation of Heavy Metals in Agricultural Soils in China: A Comprehensive Review and Meta-Analysis
Authors: Wei Anni , Jia Jin , Chang Pengyan , Wang Songliang
Published: February 2024

To control heavy metal pollution effectively, prevention of heavy metal accumulations in agricultural soils should be the priority rather than remediation of heavy metal contamination. In this research, papers which contained input and output fluxes of Cd, As, Cr, Hg, and Pb in topsoil (the plough layer) of agricultural lands in Hunan, Zhejiang, the Yangtze River Delta, Hainan, and China as a whole were explored. Fluxes of heavy metal species were recalculated, and future trends of pollution were predicted. Also, cases regarding the application of technologies to control the input and output of heavy metals were analyzed. Results indicated that atmospheric decomposition was the dominant input source of heavy metals in all study sites except Hainan. The relative contributions of fertilizers, irrigation water, and straw returning fluctuated greatly among different sites. Cd pollution in all sites was the most serious, followed by Cr and Pb. In Hunan, Cd has already exceeded the maximum limit value and needs to be controlled urgently. The input of heavy metals from irrigation water, fertilizers, and straw returning could be controlled by proposing more policies to manage their quality and application amounts. The amount of heavy metals absorbed by plants could be increased by cultivating crops with hyperaccumulators and high-biomass plants.
Achieving the Rewards of Smart Agriculture
Authors: Zhang Jian , Trautman Dawn , Liu Yingnan , Bi Chunguang , Chen Wei , Ou Lijun , Goebel Randy
Published: February 2024

From connected sensors in soils, on animals or crops, and on drones, to various software and services that are available, “smart” technologies are changing the way farming is carried out. These technologies allow producers to look beyond what the eye can see by collecting non-traditional data and then using analytics tools to improve both food sustainability and profitability. “Smart Agriculture/farming” (SA) or “Digital Agriculture” (DA), often used interchangeably, refer to precision agriculture that is thus connected in a network of sensing and acting. It is a concept that employs modern information technologies, precision climate information, and crop/livestock developmental information to connect production variables to increase the quantity and quality of agricultural and food products. This is achieved by measuring and analyzing variables accurately, feeding the information into the cloud from edge devices, extracting trends from the various data, and subsequently providing information back to the producer in a timely manner. Smart agriculture covers many disciplines, including biology, mechanical engineering, automation, machine learning, artificial intelligence, and information technology-digital platforms. Minimum standards have been proposed for stakeholders with the aim to move toward this highly anticipated and ever-changing revolution. These foundational standards encompass the following general categories, including precise articulation of objectives, and baseline standards for the Internet of Things (IoT), including network infrastructure (e.g., stable 4G or 5G networks or a wireless local area network (WLAN) are available to end users). To sum up, SA aims to improve production efficiency, enhance the quality and quantity of agricultural products, reduce costs, and improve the environmental footprint of the industry. SA’s ecosystem should be industry self-governed and collaboratively financed. SA stakeholders and end-users’ facilities should meet standard equipment requirements, such as sensor accuracy, end data collectors, relevant industry compliant software, and trusted data analytics. The SA user is willing to be part of the SA ecosystem. This short perspective aims to summarize digital/smart agriculture concept in plain language.
Assessing Panonychus ulmi (Acari: Tetranychidae) Infestations and Their Key Predators on Malus domestica Borkh in Varied Ecological Settings
Authors: Assouguem Amine , Lahlali Rachid , Joutei Abdelmalek Boutaleb , Kara Mohammed , Bari Ahmed , Aberkani Kamal , Kaur Sawinder , Mokrini Fouad , Lazraq Abderrahim
Published: February 2024

This study investigates the infestation dynamics of Panonychus ulmi McGregor 1916 and its associated predators in Malus domestica borkh orchards across three towns in Morocco: Midelt, Ait Sebaa, and Imouzzer-Kender, situated in the Fez-Meknes and Draa-Tafilalt regions. Ait Sebaa (Fez-Meknes region), characterized by high temperatures and a lower altitude, exhibited a substantial increase in P. ulmi and its predators. In contrast, Midelt (Draa-Tafilalt region), with the highest altitude, demonstrated lower mite counts. Imouzzer-Kender (Fez-Meknes region), at an intermediate altitude, showed moderate mite levels. Detailed findings reveal a positive correlation between P. ulmi and temperature, as well as with phytoseiid mites. Conversely, a negative correlation exists between P. ulmi and altitude. Additionally, a positive correlation between phytoseiid mites and temperature is identified. Overall, this study provides valuable information on the ecological interactions between mites, temperature, altitude and humidity in distinct Moroccan regions, highlighting the need to take regional specificities into account in pest management strategies.
Pollen Viability, and the Photosynthetic and Enzymatic Responses of Cowpea (Vigna unguiculata (L.) Walp., Fabaceae) in the Face of Rising Air Temperature: A Problem for Food Safety
Authors: Barros Juliane Rafaele Alves , dos Santos Tatiane Cezario , Silva Elioenai Gomes Freire , da Silva Weslley Oliveira , Guimarães Miguel Julio Machado , Angelotti Francislene
Published: February 2024

Rising temperature affects agricultural production, causing food insecurity. Thus, the objective of this study was to evaluate how increased temperature influences pollen viability, photosynthetic and enzymatic responses, and their consequences on the final yield of cowpea cultivars. The cultivars BRS Acauã, BRS Guariba, BRS Gurguéia, and BRS Pajeú were used, kept in growth chambers under two temperature regimes: 24.8–30.8–37.8 °C and 20–26–33 °C. The cultivars BRS Acauã, BRS Guariba, and BRS Pajeú showed prolonged flowering and greater flower abortion, at 23.58%, 34.71%, and 25.55%, respectively, under an increase of 4.8 °C in temperature. This increase also reduced the viability of BRS Acauã and BRS Pajeú pollen by 34 and 7%, respectively. Heating increased stomatal opening and transpiration but reduced chlorophyll content. The enzymatic response varied according to cultivars and temperature. Changes in photosynthetic and enzymatic activities contribute to reducing pollen viability and productivity. BRS Acauã was the most affected, with an 82% reduction in the number of seeds and a 70% reduction in production. BRS Gurguéia maintained its production, even with an increase of 4.8 °C, and can be selected as a cultivar with the potential to tolerate high temperatures as it maintained pollen viability, with less flower abortion, with the synchrony of physiological and biochemical responses and, consequently, greater production.
Evaluation of Anatolian Water Buffalo Carcass Weights Based on a Slaughterhouse Data Collection
Authors: Ozturk Nursen , Arap Sevinc , Kocak Omur , Serva Lorenzo , Avanus Kozet , Kilic Halil Ibrahim , Magrin Luisa , Gunes Halil
Published: February 2024

This study analyzed data collected on a slaughterhouse from 2017 to 2021, belonging to five hundred and twenty one Anatolian water buffalos from different farms located in Edirne, Istanbul, and Kirklareli. Specifically, it aimed to determine the factors affecting the carcass weights and slaughter ages of the Anatolian water buffalos. The results of the study showed that the slaughter age of the buffalos was a significant determinant of their carcass weights. Meanwhile, the sex, slaughter year, and slaughter season affected the carcass weight. Differences were observed for the slaughter age regarding the sex and farm origin. Since the pricing system in local markets is based on the buffalo carcass weight, the findings of this study could be essential for farmers when determining their fattening strategies.
Behaviour Indicators of Animal Welfare in Purebred and Crossbred Yearling Beef Reared in Optimal Environmental Conditions
Authors: Marzano Alessandra , Correddu Fabio , Lunesu Mondina Francesca , Zgheib Elias , Nudda Anna , Pulina Giuseppe
Published: February 2024

The aim of this study was to monitor the behaviour of purebred and crossbred beef cattle reared in the same optimal environmental conditions according to Classyfarm®. Thirty-yearling beef 11.5 months old, including 10 Limousines (LMS), 10 Sardo-Bruna (SRB), and 10 crossbred Limousine × Sardo-Bruna (LMS × SRB), balanced for sex and body weight, were used. Animals were evaluated for five months by two trained operators by SCAN (“sternal resting”, “lateral resting”, “ central or peripheral position in the pen”, standing”, “walking”, “feeding”, “drinking”, and “ruminating) and FOCUS (“displacement for space”, “displacement for feed or water”, “play-fighting”, “self-grooming”, “allo-grooming”, “stereotyping”, and “mounting”) protocols. Feeding behaviour was monitored by a CCTV system. The application of the SCAN sampling evidenced that SRB animals preferred the “standing” activity over the LMS animals, while the LMS × SRB did not differ from them. The “standing” and “ ruminating “activities were observed mostly in females than males (p < 0.05). For behaviour parameters assessed by the FOCUS methodology, the n-events of “allo-grooming” were higher (p < 0.05) in SRB than in LMS and LMS × SRB genetic types. Males showed higher (p < 0.05) n-events than females for “play-fighting”. For feeding behaviour, the “eating concentrate” activity (expressed as n-events) was higher (p < 0.05) in SRB than LMS × SRB and LMS being intermediate (p < 0.05). The duration of “eating concentrate” (expressed in minutes) was higher (p < 0.05) in females than males. In conclusion, behaviour indicators of animal welfare did not evidence substantial differences among genetic types and between sexes reared in the same “optimal” environmental conditions. Female beef and the autochthon’s cattle breed of Sardinia, although typically hardy, showed a wide behavioural repertoire.
Immunogenicity and Loss of Effectiveness of Biologic Therapy for Inflammatory Bowel Disease Patients Due to Anti-Drug Antibody Development
Authors: Velikova Tsvetelina , Sekulovski Metodija , Peshevska-Sekulovska Monika
Published: February 2024

Many patients with inflammatory bowel disease (IBD) experience a loss of effectiveness to biologic therapy (i.e., anti-TNF therapy, etc.). Therefore, in addition to the adverse effects of the treatment, these patients also face failure to achieve and maintain remission. Immunogenicity, the process of production of antibodies to biological agents, is fundamental to the evolution of loss of response to treatment in IBD patients. The presence of these antibodies in patients is linked to decreased serum drug levels and inhibited biological activity. However, immunogenicity rates exhibit significant variability across inflammatory disease states, immunoassay formats, and time periods. In this review, we aimed to elucidate the immunogenicity and immune mechanisms of antibody formation to biologics, the loss of therapy response, clinical results of biological treatment for IBD from systematic reviews and meta-analyses, as well as to summarize the most recent strategies for overcoming immunogenicity and approaches for managing treatment failure in IBD.
Decline in ESBL Production and Carbapenem Resistance in Urinary Tract Infections among Key Bacterial Species during the COVID-19 Pandemic
Authors: Altamimi Ibraheem , Binkhamis Khalifa , Alhumimidi Abdullah , Alabdulkarim Ibrahim M. , Almugren Abdulrahman , Alhemsi Hadi , Altamimi Abdulaziz , Almazyed Abeer , Elbih Seham , Alghunaim Razan , Altamimi Abdullah
Published: February 2024

The COVID-19 pandemic has led to significant changes in healthcare practices, including increased antibiotic usage. This study aimed to investigate the impact of the pandemic on the prevalence of extended-spectrum β-lactamase (ESBL) production and carbapenem resistance among key bacterial species causing urinary tract infections (UTIs). Conducted at King Fahad Medical City in Riyadh from January 2018 to December 2022, the study analyzed urine samples from 9697 UTI patients. Patients were categorized into ‘pre-COVID-19’ and ‘during COVID-19’ groups. Bacterial isolates were identified, and antimicrobial susceptibility testing was performed following guidelines. ESBL production was detected using the Double-Disc Synergy Test. Escherichia coli and Klebsiella pneumoniae were the main pathogens. During the pandemic, ESBL production decreased in E. coli by 1.9% and in K. pneumoniae by 6.0%. Carbapenem resistance also declined, with E. coli displaying a 1.2% reduction and K. pneumoniae and Pseudomonas aeruginosa displaying 10.7% and 7.9% reductions, respectively. Notably, logistic regression analysis revealed that the odds of ESBL presence were 10% lower during the COVID-19 pandemic (OR 0.91; 95% CI 0.83–0.99; p = 0.040), and there was a significant reduction in the odds of carbapenem resistance (OR 0.43; 95% CI 0.37–0.51; p < 0.001). This study reveals a significant decrease in ESBL production and carbapenem resistance among UTI pathogens during the COVID-19 pandemic, hinting at the impact of modified antibiotic and healthcare approaches. It emphasizes the need for persistent antimicrobial resistance surveillance and policy adaptation to address resistance challenges, offering key directions for future public health actions.
Hyperbranched Polylysine Exhibits a Collaborative Enhancement of the Antibiotic Capacity to Kill Gram-Negative Pathogens
Authors: Gong Yuxin , Peng Qing , Qiao Yu , Tian Dandan , Zhang Yuwei , Xiong Xiaoyan , He Mengxin , Xu Xiaoqing , Shi Bo
Published: February 2024

In recent years, traditional antibiotic efficacy outcomes have rapidly diminished due to the advent of drug resistance, and the dose limitation value has increased due to the severe side effect of globalized healthcare. Therefore, novel strategies are required to resensitize resistant pathogens to antibiotics existing in the field and prevent the emergence of drug resistance. In this study, cationic hyperbranched polylysine (HBPL-6) was synthesized using the one-pot polymerization method. HBPL-6 exhibited excellent non-cytotoxicity and bio-solubility properties. The present study also showed that HBPL-6 altered the outer membrane (OM) integrity of Escherichia coli O157:H7, Salmonella typhimurium, and Pseudomonas aeruginosa PAO1 by improving their permeability levels. When administered at a safe dosage, HBPL-6 enhanced the accumulation of rifampicin (RIF) and erythromycin (ERY) in bacteria to restore the efficacy of the antibiotics used. Moreover, the combination of HBPL-6 with colistin (COL) reduced the antibiotic dosage, which was helpful in preventing further drug-resistance outcomes. Therefore, this research provides a new strategy for reducing the dosage of drugs used to combat Gram-negative (G−) bacteria through their synergistic effects.
Effects of Dietary Selenium Yeast Supplementation on Lactation Performance, Antioxidant Status, and Immune Responses in Lactating Donkeys
Authors: Tong Manman , Li Shuyi , Hui Fang , Meng Fanzhu , Li Li , Shi Binlin , Zhao Yanli , Guo Xiaoyu , Guo Yongmei , Yan Sumei
Published: February 2024

Donkey milk is a traditional medicinal food with various biological activities. However, its production is very low, and lactating donkeys often experience oxidative stress, leading to a further decline in milk yield. In this study, we supplemented the diets of lactating donkeys with yeast selenium (SY) to investigate its effects on lactation performance, antioxidant status, and immune responses, and we expected to determine the optimum additive level of SY in the diet. For this study, 28 healthy lactating Dezhou donkeys with days in milk (DIM, 39.93 ± 7.02 d), estimated milk yield (EMY, 3.60 ± 0.84 kg/d), and parity (2.82 ± 0.48) were selected and randomly divided into 4 groups of 7 donkeys in each: Group SY-0 (control), Group SY-0.15, Group SY-0.3, and Group SY-0.5, with selenium supplementation of 0, 0.15, 0.3, and 0.5 mg of Se/kg DM (in form of SY) to the basal diet, respectively. The results showed a dose-dependent increase in milk yield, milk component yield, milk protein production efficiency, milk production efficiency, the activities of glutathione peroxidases (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC), as well as the content of serum interleukin-10 (IL-10), white blood cells (WBC), lymphocytes (LYM), red blood cells (RBC), hematocrit, plasma selenium, and milk selenium. Conversely, it presented a dose-dependent decrease in the activity of nitric oxide synthase (iNOS) and the contents of malondialdehyde (MDA), reactive oxygen species (ROS), nitric oxide (NO), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interferon-γ (IFN-γ). In conclusion, the results confirmed that dietary supplementation with SY can improve lactation performance, antioxidant status, and immune responses in lactating donkeys, and the recommended dose of SY was 0.3 mg/kg.
Genetic and Transcriptomic Background of Oxidative Stress and Antioxidative Therapies in Late Complications of Type 2 Diabetes Mellitus: A Systematic Review
Authors: Tonin Gašper , Dolžan Vita , Klen Jasna
Published: February 2024

This systematic review extensively investigated the role of the genetic and transcriptomic factors in late complications of type 2 diabetes mellitus (T2DM) and the current approaches targeting oxidative-stress-related pathways with antioxidant therapies. To cover our broad research area, we have conducted two systematic searches, the first focusing on genetic and transcriptomic factors affecting oxidative stress and the second one focusing on the antioxidant therapies in late complications of T2DM. The final review included 33 genetic and transcriptomic studies and 23 interventional randomized clinical trials. The conducted systematic review highlights the important role of oxidative stress in the development of late complications in T2DM patients. However, the current level of evidence does not support the use of genetic and transcriptomic factors as predictive and prognostic biomarkers for the development of T2DM late complications. Further studies are needed to elucidate the potential of targeting oxidative-stress-related pathways for novel preventative and therapeutic approaches. Additionally, antioxidants both in dietary and supplement form have been shown to improve different metabolic and biochemical parameters in T2DM patients with developed late complications. In recent years, studies have improved in methodological quality despite still mainly focusing on microvascular late complications of T2DM. Furthermore, the observed interventional studies suggest non-homogeneity in the duration of observation. As many studies do not provide post-intervention follow-up testing, it is difficult to assess the long-term health benefits of antioxidant supplementation.
Ischemia-Modified Albumin (IMA) Is Associated with Poor Survival in Patients with Newly Diagnosed Idiopathic Pulmonary Fibrosis (IPF): A Pilot Study
Authors: Zinellu Angelo , Zoroddu Stefano , Fois Simona , Mellino Sabrina , Scala Chiara , Virdis Erika , Zinellu Elisabetta , Sotgia Salvatore , Paliogiannis Panagiotis , Mangoni Arduino A. , Carru Ciriaco , Pirina Pietro , Fois Alessandro G.
Published: February 2024

There are increasing efforts to better predict adverse outcomes for idiopathic pulmonary fibrosis (IPF). Our aim was to assess the prognostic potential of ischemia-modified albumin (IMA), an established circulating marker of ischemia and, more recently, oxidative stress, in a cohort of 56 IPF patients recruited between 2015 and 2023 at the University of Sassari, Italy. Demographic and functional parameters and serum IMA concentrations were measured at baseline. Non-survivors had significantly higher IMA concentrations vs. survivors (508 ± 64 vs. 474 ± 42 mABSU, respectively; p = 0.035). The Kaplan–Meier analysis showed a significant association between higher IMA values and poor survival (HR: 3.32, 95% CI from 1.06 to 10.4, p = 0.039). In the Cox regression analysis, this association remained significant after adjusting for the force expiratory volume at 1 s, the total lung capacity, lymphocyte count, and pharmacological treatment (HR: 1.0154, 95% CI from 1.0035 to 1.0275, p = 0.01). IMA, an oxidative stress biomarker measurable using relatively simple and available methods, is independently associated with mortality in IPF. Therefore, its determination may enhance risk stratification and treatment decisions. Prospective studies involving larger cohorts are needed to confirm this association and to endorse the use of IMA in routine practice.
Intratracheal Administration of Stem Cell Membrane-Cloaked Naringin-Loaded Biomimetic Nanoparticles Promotes Resolution of Acute Lung Injury
Authors: Jin Hua , Zhao Yue , Yao Yinlian , Fan Shilong , Luo Renxing , Shen Xin , Wang Yanyan , Pi Jiang , Huang Gonghua
Published: February 2024

Cytokine storm and ROS overproduction in the lung always lead to acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) in a very short time. Effectively controlling cytokine storm release syndrome (CRS) and scavenging ROS are key to the prevention and treatment of ALI/ARDS. In this work, the naringin nanoparticles (Nar-NPs) were prepared by the emulsification and evaporation method; then, the mesenchymal stem cell membranes (CMs) were extracted and coated onto the surface of the Nar-NPs through the hand extrusion method to obtain the biomimetic CM@Nar-NPs. In vitro, the CM@Nar-NPs showed good dispersity, excellent biocompatibility, and biosafety. At the cellular level, the CM@Nar-NPs had excellent abilities to target inflamed macrophages and the capacity to scavenge ROS. In vivo imaging demonstrated that the CM@Nar-NPs could target and accumulate in the inflammatory lungs. In an ALI mouse model, intratracheal (i.t.) instillation of the CM@Nar-NPs significantly decreased the ROS level, inhibited the proinflammatory cytokines, and remarkably promoted the survival rate. Additionally, the CM@Nar-NPs increased the expression of M2 marker (CD206), and decreased the expression of M1 marker (F4/80) in septic mice, suggesting that the Nar-modulated macrophages polarized towards the M2 subtype. Collectively, this work proves that a mesenchymal stem cell membrane-based biomimetic nanoparticle delivery system could efficiently target lung inflammation via i.t. administration; the released payload inhibited the production of inflammatory cytokines and ROS, and the Nar-modulated macrophages polarized towards the M2 phenotype which might contribute to their anti-inflammation effects. This nano-system provides an excellent pneumonia-treated platform with satisfactory biosafety and has great potential to effectively deliver herbal medicine.
Alterations in Mitochondrial Oxidative Phosphorylation System: Relationship of Complex V and Cardiac Dysfunction in Human Heart Failure
Authors: Giménez-Escamilla Isaac , Benedicto Carlota , Pérez-Carrillo Lorena , Delgado-Arija Marta , González-Torrent Irene , Vilchez Roger , Martínez-Dolz Luis , Portolés Manuel , Tarazón Estefanía , Roselló-Lletí Esther
Published: February 2024

Heart failure (HF) is a disease related to bioenergetic mitochondrial abnormalities. However, the whole status of molecules involved in the oxidative phosphorylation system (OXPHOS) is unknown. Therefore, we analyzed the OXPHOS transcriptome of human cardiac tissue by RNA-seq analyses (mRNA n = 36; ncRNA n = 30) in HF patients (ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM)) and control subjects. We detected 28 altered genes in these patients, highlighting greater deregulation in ICM. Specifically, we found a general overexpression of complex V (ATP synthase) elements, among them, ATP5I (ICM, FC = 2.04; p < 0.01), ATP5MJ (ICM, FC = 1.33, p < 0.05), and ATP5IF1 (ICM, FC = 1.81; p < 0.001), which presented a significant correlation with established echocardiographic parameters of cardiac remodeling and ventricular function as follows: left ventricular end-systolic (p < 0.01) and end-diastolic (p < 0.01) diameters, and ejection fraction (p < 0.05). We also detected an increase in ATP5IF1 protein levels (ICM, FC = 1.75; p < 0.01) and alterations in the microRNA expression levels of miR-208b-3p (ICM, FC = −1.44, p < 0.001), miR-483-3p (ICM, FC = 1.37, p < 0.01), regulators of ATP5I. Therefore, we observed the deregulation of the OXPHOS transcriptome in ICM patients, highlighting the overexpression of complex V and its relationship with cardiac remodeling and function.
Antioxidant Activity of Essential Oils from Pinaceae Species
Authors: Ancuceanu Robert , Anghel Adriana Iuliana , Hovaneț Marilena Viorica , Ciobanu Anne-Marie , Lascu Beatrice Elena , Dinu Mihaela
Published: February 2024

With a widespread distribution throughout the Northern Hemisphere and 11 genera, Pinaceae is the largest family of Gymnosperms in the world. Essential oils are an important chemotaxonomic marker for the species of this family, although the degree of chemical and biological investigation has not been the same for all genera. Essential oils from Abies and Cedrus (from the abietoid clade) or Pinus and Picea (from the pinoid clade) have been more extensively investigated with respect to their chemical composition and biological or pharmacological properties, including their antioxidant effects. Instead, essential oils from the other genera of the family have been less explored in this respect or even have not been investigated at all. This is a narrative review looking into the knowledge acquired up to date, the variability and limitations of the current methods used to estimate antioxidant effects, and multiple comparisons between EOs obtained from different genera, species, and plant parts, as well as potential applications and future directions of research and utilization of essential oils derived from Pinaceae species.
Redox System and Oxidative Stress-Targeted Therapeutic Approaches in Bladder Cancer
Authors: Dugbartey George J. , Relouw Sydney , McFarlane Liam , Sener Alp
Published: February 2024

Bladder cancer (BCa) is the most common genitourinary malignancy, with a high global incidence and recurrence rate that is paired with an increasing caregiver burden and higher financial cost, in addition to increasing morbidity and mortality worldwide. Histologically, BCa is categorized into non-muscle invasive, muscle invasive, and metastatic BCa, on the basis of which the therapeutic strategy is determined. Despite all innovations and recent advances in BCa research, conventional therapies such as chemotherapy, immunotherapy, radiotherapy, and surgery fall short in the complete management of this important malignancy. Besides this worrying trend, the molecular basis of BCa development also remains poorly understood. Burgeoning evidence from experimental and clinical studies suggests that oxidative stress resulting from an imbalance between reactive oxygen species (ROS) generation and the body’s antioxidant production plays an integral role in BCa development and progression. Hence, ROS-induced oxidative stress-related pathways are currently under investigation as potential therapeutic targets of BCa. This review focuses on our current understanding regarding ROS-associated pathways in BCa pathogenesis and progression, as well as on antioxidants as potential adjuvants to conventional BCa therapy.
Genomic Alterations in Melanocytic Tumors: A Review of Spitz Tumors, Blue Nevi, Deep Penetrating Melanocytomas and Pigmented Epithelioid Melanocytomas
Authors: Saade Rayan , Al-Rohil Rami N.
Published: February 2024

The arena of melanocytic histopathology has experienced tremendous growth in the last decade. The advancement is attributed to incorporating various molecular tests in benign, intermediate, and malignant melanocytic tumors. Most molecular testing has been mainly applied in clinically advanced-stage melanoma to determine the molecular alteration to help guide therapy (e.g., BRAF inhibitors in BRAF mutated melanomas). However, with more availability and, to a certain degree, affordability of certain molecular tests, multiple studies have been conducted on benign/intermediate lesions in an attempt to understand further the driving molecular alterations allowing for the proliferation of certain melanocytic lineages. This review article discusses and illustrates examples of recently recognized entities with their corresponding genomic alterations in the Spitz lineage, blue nevi, deep penetrating melanocytomas, and pigmented epithelioid melanocytomas.
Assessment of the Nutritional Potential and Resistance to Oxidation of Sea Buckthorn and Rosehip Oils
Authors: Wirkowska-Wojdyła Magdalena , Ostrowska-Ligęza Ewa , Górska Agata , Brzezińska Rita , Piasecka Iga
Published: February 2024

Cold-pressed oils from non-typical materials, such as wild rose and sea buckthorn, should meet certain requirements to be approved for consumption. The aim of this study was to evaluate the nutritional potential and selected quality parameters with particular emphasis on oxidative stability of two commercially available cold-pressed oils: rose hip oil and sea buckthorn oil. The fatty acid composition, including nutritional indexes (polyunsaturated fatty acid/saturated fatty acid ratio PUFA/SFA; atherogenicity index IA is as follows: hypocholesterolemic/hypercholesterolemic index HH; health-promoting index HPI), positional distribution of fatty acids, melting profile and oxidative stability were analyzed. The tested oils were characterized by a high content of unsaturated fatty acids, which were mainly represented by oleic and linoleic acids. In the case of one of the analyzed rosehip oil oils (R1), α-linolenic acid was also found at the level of 26%, which contributed to obtaining the recommended ratio of omega-6 to omega-3 fatty acids. The lowest value of IA and the highest values of HH and HPI were also recorded for R1 rosehip oil. This oil was also characterized by the highest resistance to oxidation, as indicated by induction times measured at various temperatures. The distribution of fatty acids in triacylglycerols molecules in the analyzed oils was typical for vegetable oils. In the case of tested oils, similar melting profiles with endothermic peaks recorded at negative temperatures, which resulted from the presence of triacylglycerol fractions with a high proportion of mono- and polyunsaturated fatty acids, were observed.
Deep Learning (Fast R-CNN)-Based Evaluation of Rail Surface Defects
Authors: Choi Jung-Youl , Han Jae-Min
Published: February 2024

In current railway rails, trains are propelled by the rolling contact between iron wheels and iron rails, and the high frequency of train repetition on rails results in a significant load exertion on a very small area where the wheel and rail come into contact. Furthermore, a contact stress beyond the allowable stress of the rail may lead to cracks due to plastic deformation. The railway rail, which is the primary contact surface between the wheel and the rail, is prone to rolling contact fatigue cracks. Therefore, a thorough inspection and diagnosis of the condition of the cracks is necessary to prevent fracture. The Detailed Guideline on the Performance Evaluation of Track Facilities in South Korea specifies the detailed requirements for the methods and procedures for conducting track performance evaluations. However, diagnosing rail surface damage and determining the severity solely rely on visual inspection, which depends on the qualitative evaluation and subjective judgment of the inspector. Against this backdrop, rail surface defect detection was investigated using Fast R-CNN in this study. To test the feasibility of the model, we constructed a dataset of rail surface defect images. Through field investigation, 1300 images of rail surface defects were obtained. Aged rails collected from the field were processed, and 1300 images of internal defects were generated through SEM testing; therefore, a total of 1300 pieces of learning data were constructed. The detection results indicated that the mean average precision was 94.9%. The Fast R-CNN exhibited high efficiency in detecting rail surface defects, and it demonstrated a superior recognition performance compared with other algorithms.