bluuebunny/crossref_metadata_2025 huggingface dataset:
Retinoprotection by BGP-15, a Hydroximic Acid Derivative, in a Type II Diabetic Rat Model Compared to Glibenclamide, Metformin, and Pioglitazone
Authors: Wachal Zita , Bombicz Mariann , Priksz Dániel , Hegedűs Csaba , Kovács Diána , Szabó Adrienn Mónika , Kiss Rita , Németh József , Juhász Béla , Szilvássy Zoltán , Varga Balázs
Published: March 2020

High blood glucose and the consequential ischemia-reperfusion (I/R) injury damage vessels of the retina, deteriorating its function, which can be clearly visualized by electroretinography (ERG). The aim of the present study was to evaluate the possible retinoprotective effects of systemic BGP-15, an emerging drug candidate, in an insulin resistant animal model, the Goto-Kakizaki rat, and compare these results with well-known anti-diabetics such as glibenclamide, metformin, and pioglitazone, which even led to some novel conclusions about these well-known agents. Experiments were carried out on diseased animal model (Goto-Kakizaki rats). The used methods include weight measurement, glucose-related measurements—like fasting blood sugar analysis, oral glucose tolerance test, hyperinsulinemic euglycemic glucose clamp (HEGC), and calculations of different indices from HEGC results—electroretinography and Western Blot. Beside its apparent insulin sensitization, BGP-15 was also able to counteract the retina-damaging effect of Type II diabetes comparable to the aforementioned anti-diabetics. The mechanism of retinoprotective action may include sirtuin 1 (SIRT1) and matrix metalloproteinase 9 (MMP9) enzymes, as BGP-15 was able to elevate SIRT1 and decrease MMP9 expression in the eye. Based on our results, this emerging hydroximic acid derivative might be a future target of pharmacological developments as a potential drug against the harmful consequences of diabetes, such as diabetic retinopathy.
H3K4me3 Is a Potential Mediator for Antiproliferative Effects of Calcitriol (1α,25(OH)2D3) in Ovarian Cancer Biology
Authors: Han Nan , Jeschke Udo , Kuhn Christina , Hester Anna , Czogalla Bastian , Mahner Sven , Rottmann Miriam , Mayr Doris , Schmoeckel Elisa , Trillsch Fabian
Published: March 2020

Posttranslational histone modification plays an important role in tumorigenesis. Histone modification is a dynamic response of chromatin to various signals, such as the exposure to calcitriol (1α,25(OH)2D3). Recent studies suggested that histone modification levels could be used to predict patient outcomes in various cancers. Our study evaluated the expression level of histone 3 lysine 4 trimethylation (H3K4me3) in a cohort of 156 epithelial ovarian cancer (EOC) cases by immunohistochemical staining and analyzed its correlation to patient prognosis. The influence of 1α,25(OH)2D3 on the proliferation of ovarian cancer cells was measured by BrdU proliferation assay in vitro. We could show that higher levels of H3K4me3 were correlated with improved overall survival (median overall survival (OS) not reached vs. 37.0 months, p = 0.047) and identified H3K4me3 as a potential prognostic factor for the present cohort. Ovarian cancer cell 1α,25(OH)2D3 treatment induced H3K4me3 protein expression and exhibited antiproliferative effects. By this, the study suggests a possible impact of H3K4me3 expression on EOC progression as well as its relation to calcitriol (1α,25(OH)2D3) treatment. These results may serve as an explanation on how 1α,25(OH)2D3 mediates its known antiproliferative effects. In addition, they further underline the potential benefit of 1α,25(OH)2D3 supplementation in context of ovarian cancer care.
Smoking Upregulates Angiotensin-Converting Enzyme-2 Receptor: A Potential Adhesion Site for Novel Coronavirus SARS-CoV-2 (Covid-19)
Authors: Brake Samuel James , Barnsley Kathryn , Lu Wenying , McAlinden Kielan Darcy , Eapen Mathew Suji , Sohal Sukhwinder Singh
Published: March 2020

The epicenter of the original outbreak in China has high male smoking rates of around 50%, and early reported death rates have an emphasis on older males, therefore the likelihood of smokers being overrepresented in fatalities is high. In Iran, China, Italy, and South Korea, female smoking rates are much lower than males. Fewer females have contracted the virus. If this analysis is correct, then Indonesia would be expected to begin experiencing high rates of Covid-19 because its male smoking rate is over 60% (Tobacco Atlas). Smokers are vulnerable to respiratory viruses. Smoking can upregulate angiotensin-converting enzyme-2 (ACE2) receptor, the known receptor for both the severe acute respiratory syndrome (SARS)-coronavirus (SARS-CoV) and the human respiratory coronavirus NL638. This could also be true for new electronic smoking devices such as electronic cigarettes and “heat-not-burn” IQOS devices. ACE2 could be a novel adhesion molecule for SARS-CoV-2 causing Covid-19 and a potential therapeutic target for the prevention of fatal microbial infections, and therefore it should be fast tracked and prioritized for research and investigation. Data on smoking status should be collected on all identified cases of Covid-19.
A Novel Predictive Model for In-Hospital Mortality Based on a Combination of Multiple Blood Variables in Patients with ST-Segment-Elevation Myocardial Infarction
Authors: Goriki Yuhei , Tanaka Atsushi , Nishihira Kensaku , Kawaguchi Atsushi , Natsuaki Masahiro , Watanabe Nozomi , Ashikaga Keiichi , Kuriyama Nehiro , Shibata Yoshisato , Node Koichi
Published: March 2020

In emergency clinical settings, it may be beneficial to use rapidly measured objective variables for the risk assessment for patient outcome. This study sought to develop an easy-to-measure and objective risk-score prediction model for in-hospital mortality in patients with ST-segment elevation myocardial infarction (STEMI). A total of 1027 consecutive STEMI patients were recruited and divided into derivation (n = 669) and validation (n = 358) cohorts. A risk-score model was created based on the combination of blood test parameters obtained immediately after admission. In the derivation cohort, multivariate analysis showed that the following 5 variables were significantly associated with in-hospital death: estimated glomerular filtration rate <45 mL/min/1.73 m2, platelet count <15 × 104/μL, albumin ≤3.5 g/dL, high-sensitivity troponin I >1.6 ng/mL, and blood sugar ≥200 mg/dL. The risk score was weighted for those variables according to their odds ratios. An incremental change in the scores was significantly associated with elevated in-hospital mortality (p < 0.001). Receiver operating characteristic curve analysis showed adequate discrimination between patients with and without in-hospital death (derivation cohort: area under the curve (AUC) 0.853; validation cohort: AUC 0.879), and there was no significant difference in the AUC values between the laboratory-based and Global Registry of Acute Coronary Events (GRACE) score (p = 0.721). Thus, our laboratory-based model might be helpful in objectively and accurately predicting in-hospital mortality in STEMI patients.
Effects of Contemporary Irrigant Activation Schemes and Subsequent Placement of an Interim Dressing on Bacterial Presence and Activity in Root Canals Associated with Asymptomatic Apical Periodontitis
Authors: Carvalho Alexandre P. L. , Nardello Laura C. L. , Fernandes Fernanda S. , Bruno Fernanda P. , Paz Luiza R. , Iglecias Elaine F. , Honório Heitor M. , Mayer Márcia P. A. , Gavini Giulio , Pinheiro Ericka T.
Published: March 2020

New tools for activating endodontic irrigants have evolved, yet their impact on root canal disinfection, in comparison to the passive placing of an inter-visit medication, have not yet been fully elucidated. The use of DNA- and rRNA-based methods may cast some new light on this issue, as they allow a comparison to be made between microbial presence and activity. Therefore, the aim of this single-arm intervention trial is to evaluate the antibacterial effect of endodontic procedures using both molecular methods. Root canal samples were obtained from 20 patients with asymptomatic apical periodontitis after each treatment step: access cavity, chemo-mechanical preparation, adjunctive procedures (XP-endo Finisher file and passive ultrasonic irrigation), calcium hydroxide medication, and 2nd-visit root canal preparation. DNA and cDNA from the samples were subjected to quantitative polymerase chain reaction with universal primers for the bacterial 16S rRNA gene. Chemo-mechanical preparation promoted a drastic reduction in bacterial levels and activity, whereas the adjunctive procedures did not make a significant contribution to further disinfection. At the 2nd visit, bacteria were active after the use of calcium hydroxide medication; however, they were significantly reduced after a 2nd-visit preparation. Consequently, the lowest bacterial levels were found at the end of the treatment. This clinical trial, which used an rRNA and rDNA combined approach, confirmed previous studies showing that root canal preparation represents the main strategy for root canal disinfection.
Clinical Characteristics and Outcome of Patients with Infective Endocarditis Diagnosed in a Department of Internal Medicine
Authors: Kreitmann Louis , Montaigne David , Launay David , Morell-Dubois Sandrine , Maillard Hélène , Lambert Marc , Hachulla Eric , Sobanski Vincent
Published: March 2020

Clinical manifestations of infective endocarditis (IE) can be highly non-specific. Our objective was to describe the clinical characteristics of patients initially referred to a department of internal medicine for a diagnostic work-up, and eventually diagnosed with IE. We retrospectively retrieved adult patients admitted to the department of internal medicine at Lille University Hospital between 2004 and 2015 who fulfilled Duke Classification criteria for definite IE. Thirty-five patients were included. The most frequently involved bacteria were non-hemolytic streptococci. Most patients presented with various systemic, cardiac, embolic, rheumatic, and immunological findings, with no sign or symptom displaying high sensitivity. The first transthoracic echocardiogram was negative in 42% of patients. Furthermore, definite diagnosis required performing at least 2 transesophageal examinations in 24% of patients. We observed a trend towards decreased survival in the subgroup of patients in whom the delay between onset of symptoms and diagnosis was >30 days. In conclusion, patients who are initially referred to internal medicine for a diagnosis work-up and who are ultimately diagnosed with IE have non-specific symptoms and a high percentage of initial normal echocardiography. Those patients require prolonged echocardiographic monitoring as a prolonged delay in diagnosis is associated with poorer outcomes such as death.
Endoscopic Ultrasound-Guided Treatments for Non-Variceal Upper GI Bleeding: A Review of the Literature
Authors: De Angelis Claudio Giovanni , Cortegoso Valdivia Pablo , Rizza Stefano , Venezia Ludovica , Rizzi Felice , Gesualdo Marcantonio , Saracco Giorgio Maria , Pellicano Rinaldo
Published: March 2020

Endoscopic injection of glues, clotting factors, or sclerosing agents is a well-known therapy for the treatment of non-variceal upper gastrointestinal bleeding (NVUGIB), but less is known about endoscopic ultrasound (EUS)-guided treatments. In this setting, literature data are scarce, and no randomized controlled trials are available. We performed a review of the existing literature in order to evaluate the role of EUS-guided therapies in the management of NVUGIB. The most common treated lesions were Dieulafoy’s lesions, pancreatic pseudoaneurysms, and gastrointestinal stromal tumors (GISTs). Mostly, the treatments were performed as a salvage option after failure of conventional endoscopic hemostatic attempts, showing good efficacy and a good safety profile, also documented by Doppler monitoring of treated lesions. EUS-guided therapies may be an effective option in the treatment of refractory NVUGIB, thus avoiding radiological or surgical management. Nevertheless, available literature still lacks robust data.
Effects of Self-Care for Older PErsons (SCOPE) on Functional and Physiological Measures: A Cluster Randomized Controlled Trial
Authors: Ng Ted Kheng Siang , Matchar David Bruce , Sultana Rehena , Chan Angelique
Published: March 2020

Background: Population aging poses unprecedented demands on the healthcare system. There is also a scarcity of evidence on self-care intervention to improve objective measures of morbidity and aging-associated functional and physiological measures in a low-income multi-ethnic population setting. Methods: We conducted a cluster randomized controlled trial (ClinicalTrials.gov Identifier: NCT01672177) to examine the effects of the Self-Care for Older PErsons (SCOPE) program. We randomized 14 Senior Activity Centers and randomly selected older adults within these centers. Functional and physiological measurements were performed at baseline, 10-month, and 18-month periods. The primary outcome was a composite of three morbidity-specific measures, which include hemoglobin A1c (HbA1C), peak expiratory flow, and systolic blood pressure. Aging-associated functional and physiological measures were examined as secondary outcomes. Repeated-measure mixed models were employed to examine the effects of SCOPE on these measures. Results: 378 community-dwelling older adults participated in either the treatment (n= 164) or the control arm (n = 214). The primary outcome was not significantly improved. For the secondary outcomes, SCOPE participants demonstrated slower oxygen desaturation at an 18-month period (p = 0.001), improved time to complete the chair-stand test (p < 0.001) at a 10-month period with the effect persisting at the 18-month period (p < 0.001). SCOPE participants also had significantly improved vitamin B12 levels at the 18-month period (p < 0.001), increased hemoglobin concentration (p < 0.001), decreased mean corpuscular volume (p = 0.001), and decreased creatinine (p = 0.002) at the 10-month period. Conclusions: SCOPE did not improve morbidity-specific measures. However, it improved several aging-associated measures implicated in geriatric syndromes. This study highlights the potential of a self-care program in the prevention of geriatric syndromes in community-dwelling older adults, while emphasizing self-management to manage existing morbidities.
Aryl Hydrocarbon Receptor Activation Downregulates IL-33 Expression in Keratinocytes via Ovo-Like 1
Authors: Tsuji Gaku , Hashimoto-Hachiya Akiko , Yen Vu Hai , Miake Sho , Takemura Masaki , Mitamura Yasutaka , Ito Takamichi , Murata Maho , Furue Masutaka , Nakahara Takeshi
Published: March 2020

Background: IL-33, one of the IL-1 superfamily cytokines, has been shown to be associated with pruritus and inflammation in atopic dermatitis (AD). Furthermore, IL-33 production derived from keratinocytes reportedly has a crucial role in the development of AD; however, the mechanism of IL-33 expression has not been fully understood. Methods: We analyzed IL-33 expression in normal human epidermal keratinocytes (NHEKs) treated with IL-4. Results: IL-4 induced the upregulation of IL-33 expression in NHEKs. Based on the findings 1) that ovo-like 1 (OVOL1), a susceptible gene of AD, upregulates filaggrin (FLG) and loricrin (LOR) expression in NHEKs and 2) that reduced expression of FLG and LOR leads to production of IL-1 superfamily cytokines, we examined the involvement of OVOL1 in IL-33 expression in NHEKs. Knockdown of OVOL1 induced upregulation of IL-33 expression. Moreover, because Glyteer, an activator of aryl hydrocarbon receptor (AHR), reportedly upregulates OVOL1 expression, we examined whether treatment with Glyteer inhibited IL-33 expression in NHEKs. Treatment with Glyteer inhibited IL-4-induced upregulation of IL-33 expression, which was canceled by knockdown of either AHR or OVOL1. Conclusions: Activation of the AHR-OVOL1 axis inhibits IL-4-induced IL-33 expression, which could be beneficial for the treatment of AD.
The Emergent Behaviour of Thermal Networks and Its Impact on the Thermal Conductivity of Heterogeneous Materials and Systems
Authors: Bowen Chris R. , Robinson Kevin , Tian Jianhui , Zhang Meijie , Coveney Vincent A. , Xia Qiulin , Lock Gary
Published: March 2020

The properties of thermal networks are examined to understand the effective thermal conductivity of heterogeneous two-phase composite materials and systems. At conditions of high contrast in thermal conductivity of the individual phases (k1 and k2), where k1 << k2 or k1 >> k2, the effective thermal conductivity of individual networks of the same composition was seen to be highly sensitive to the distribution of the phases and the presence of percolation paths across the network. However, when the contrast in thermal conductivities of the two phases was modest (k1/k2 ~ 10−2 to 102), the thermal networks were observed to exhibit an emergent response with a low variability in the effective thermal conductivity of mixtures of the same composition. A logarithmic mixing rule is presented to predict the network response in the low variability region. Excellent agreement between the model, mixing rule and experimental data is observed for a range two-phase porous and granular media. The modelling approach provides new insights into the design of multi-phase composites for thermal management applications and the interpretation or prediction of their heat transfer properties.
Survey of Community Livelihoods and Landscape Change along the Nzhelele and Levuvhu River Catchments in Limpopo Province, South Africa
Authors: Musakwa Walter , Wang Shuai , Wei Fangli , Malapane Olgah Lerato , Thomas Masala Makumule , Mavengahama Sydney , Zeng Hongwei , Wu Bingfang , Zhao Wenwu , Nyathi Nesisa Analisa , Mashimbye Zama Eric , Poona Nitesh , Chakwizira James , Gumbo Trynos , Mokoena Baleseng , Kaitano Francis , Fundisi Emmanuel , Yeni-Letsoko Vuyiswa
Published: March 2020

Landscape-change studies have attracted increasing interest because of their importance to land management and the sustainable livelihoods of rural communities. However, empirical studies on landscape change and its drivers are often poorly understood, particularly, in small rural communities in developing countries such as South Africa. The present study surveyed local community livelihoods and perceptions of landscape change in the Nzhelele and Levuvhu river catchments in Limpopo Province, South Africa. These areas have experienced land reform and are also characterized by environmental degradation, poverty, inequality and environmental justice concerns among other issues. Land-cover maps derived from Landsat satellite imagery were used for purposes of correlating and validating the survey data findings and results. The survey results showed that education levels, working status and marital status have statistically significant effects on community livelihoods (indicated by levels of income, p < 0.05). Maize, fruits and vegetables are the main cultivated crop varieties in the study area, and these crops are mainly used for subsistence to meet household self-consumption requirements. Moreover, local community members and stakeholders argue that the landscape has changed over the past 20 years mainly as a result of urban expansion, deforestation, agricultural diversification and forestry intensification. These landscape changes were largely confirmed by the land-cover change maps derived from satellite imagery. Soil erosion as a result of landscape changes was identified as a major threat and hazard in the study area. Political, natural, economic and cultural factors have been identified as the major underlying drivers for the observed landscape changes. These results have implications for understanding landscape change, coupled with human–nature relationships as well as informing government policy with respect to advancing land management and further promotion of the sustainable livelihoods of rural communities. Overall, the study proposes a multiple stakeholders’ approach and ecosystem-based approach to promote the sustainable management of landscapes in rural areas.
Applying the RUSLE and ISUM in the Tierra de Barros Vineyards (Extremadura, Spain) to Estimate Soil Mobilisation Rates
Authors: Barrena-González Jesús , Rodrigo-Comino Jesús , Gyasi-Agyei Yeboah , Pulido Fernández Manuel , Cerdà Artemi
Published: March 2020

Spain is one of the largest wine producers in the world, with Extremadura (south-west Spain) being its second-largest producing region after Castilla La Mancha. Within Extremadura, the most traditional and productive viticulture region is the Tierra de Barros, which boasts an annual production of 3×106 litres. However, no soil erosion assessment has been undertaken in any vineyard in the region to ascertain environmental sustainability. Therefore, the Improved Stock Unearthing Method (ISUM) and the Revised Universal Soil Loss Equation (RUSLE) were applied to assess the long-term soil erosion rates. Both methods were applied using an experimental plot (2.8 m × 148.5 m) encompassing 99 paired vines in a 20-year-old vineyard under a tillage management system and on bare soils throughout the year. The ISUM and RUSLE found total soil mobilization values of 45.7 Mg ha−1 yr−1 and 17.4 Mg ha−1 yr−1, respectively, a difference of about 5 times. Mapping techniques showed that soil surface declined to an average of −6.2 cm, with maximum values of −28 cm. The highest values of soil depletion were mainly observed in the upper part and the form of linear features following the hillslope direction. On the other hand, under the vines, the soil surface level showed accumulations of up to +2.37 cm due to tillage practices. Our study demonstrated the potential of high soil erosion rates occurring in conventional vineyards managed with tillage in the inter-row areas and herbicides under the vines within the Tierra de Barros. Also, we demonstrated the elevated differences in soil mobilisation rates using the ISUM and RUSLE. Therefore, further research must be conducted in other vineyards to determine the suitability of the models for assessing soil erosion rates. Undoubtedly, soil conservation measures must be designed and applied immediately due to high erosion rates.
The Factors Affecting Farmland Rental Prices in Slovakia
Authors: Takáč Ivan , Lazíková Jarmila , Rumanovská Ľubica , Bandlerová Anna , Lazíková Zuzana
Published: March 2020

Agricultural land is a limited natural resource with increasing economic value. This study analyses land rental relationships in Slovakia, including legal rental regulations, and identifies the impact of certain factors, such as the European Union Common Agricultural Policy (CAP) payments, and geographical and economic factors on land rental prices. From the results of econometric models, it was found that certain CAP payments have an effect on rental prices, mainly the single area payment scheme (SAPS), payments for agri-environmental-climate schemes (AECS), and animal welfare, which were found to have positive effects. Other important factors found to influence rental prices are economic indicators (such as total revenue share of total costs, share of revenue from agricultural production in terms of total revenue, share of production costs as a percentage of total costs, wages, and number of employees) and geographical factors (such as region or partial production areas). However, the distance of the farm from the district city (LAU 1) and the share of farmland affected by natural constraints do not considerably affect rental prices in Slovakia. Land consolidation is a statistically significant factor according to the models; however, its impact is almost zero. Knowledge of these factors constitutes important know-how, not only for policy makers but also for the actors operating in the land rental market (e.g., landlords, tenants, experts on land valuation, and real estate agents).
Linear Elastic FE-Analysis of Porous, Laser Welded, Heat Treatable, Aluminium High Pressure Die Castings Based on X-Ray Computed Tomography Data
Authors: Teichmann Fabian , Ziemer Arne , Leitner Martin , Hensel Jonas , Dilger Klaus
Published: March 2020

The welding of aluminium high pressure die castings is a well known and broadly investigated challenge in various fields of industry and research. Prior research in this specific field mainly focused on the optimisation of the welding and the casting process and on the cause of the frequently occurring porosity and incomplete fusion phenomena, whereas the impacts of these defects have hardly been addressed. Therefore, the underlying study presents the investigation of weldments in EN AC-AlSi10MnMg high pressure aluminium die castings by linear elastic finite element analysis based on X-ray computed tomography as a novel approach. Hereby, four laser weldments with differing surfaces and pore contents were investigated by X-ray computed tomography and tensile testing. Based on the voxel datasets of the porous weldments, triangular finite element meshes were generated and a numerical finite element analysis was conducted. Good agreement of the stress–strain curves between the simulations and the experiments was achieved.
Impact of Cement Dust Pollution on the Surface of Sound-Absorbing Panels on Their Acoustic Properties
Authors: Nowoświat Artur , Dulak Leszek
Published: March 2020

The publication presents a comparison of the sound absorption test results of a perforated wall cassette filled with mineral wool for various degree of cement dust pollution. Cement dust should be understood here as dust created during the production of cement and during the milling and dispatch of finished products. If the partitions in production plants are made of sound-absorbing cassettes or additional sound-absorbing elements made of perforated cassettes are applied, we must know how dust can change sound-absorbing properties of the cassettes. Thus, one has to consider whether the use of sound-absorbing perforated cassettes is appropriate if sound-absorbing parameters change over time due to dust. To determine the impact of dust-covered perforation on sound-absorbing parameters, tests were performed for four variants having different level of pollution. The tests involved ‘clean’ and then dust-covered cassettes, each time increasing the amount of cement dust on the perforations. Sound absorption parameters of the cassettes were tested in the reverberation chamber for individual variants. Test results indicate the loss of sound absorption of the cassettes only when they are heavily polluted. Then the reduction of the single-number sound absorption index αw is 50%. Using computer simulation, we analyzed how the change of sound-absorbing parameters of the cassettes would influence the change of noise reduction in the production hall. The results of the analysis demonstrate a very effective reduction of noise level of 14 dB by the application of clean cassettes. The reduction value for the dirtiest cassettes was 6 dB.
Fabrication, Characterization and Implementation of Thermo Resistive TiCu(N,O) Thin Films in a Polymer Injection Mold
Authors: Oliveira Eva , Silva João Paulo , Laranjeira Jorge , Macedo Francisco , Lanceros-Mendez Senentxu , Vaz Filipe , Ferreira Armando
Published: March 2020

This paper presents the development of metallic thermoresistive thin film, providing an innovative solution to dynamically control the temperature during the injection molding process of polymeric parts. The general idea was to tailor the signal response of the nitrogen- and oxygen-doped titanium-copper thin film (TiCu(N,O))-based transducers, in order to optimize their use in temperature sensor devices. The results reveal that the nitrogen or oxygen doping level has an evident effect on the thermoresistive response of TiCu(N,O) films. The temperature coefficient of resistance values reached 2.29 × 10−2 °C−1, which was almost six times higher than the traditional platinum-based sensors. In order to demonstrate the sensing capabilities of thin films, a proof-of-concept experiment was carried out, integrating the developed TiCu(N,O) films with the best response in an injection steel mold, connected to a data acquisition system. These novel sensor inserts proved to be sensitive to the temperature evolution during the injection process, directly in contact with the polymer melt in the mold, demonstrating their possible use in real operation devices where temperature profiles are a major parameter, such as the injection molding process of polymeric parts.
Recent Advances on the Application of Layered Double Hydroxides in Concrete—A Review
Authors: Mir Zahid M. , Bastos Alexandre , Höche Daniel , Zheludkevich Mikhail L.
Published: March 2020

The issue of chloride induced corrosion of reinforced concrete is a serious problem affecting infrastructure globally and causing huge economic losses. As such this issue has gained a considerable attention in the scientific community in the recent past. Layered Double Hydroxides (LDHs) have recently emerged as a new class of concrete-additives with a potential to increase the chloride resistance of concrete and mitigate corrosion. LDHs are clay like structures consisting of positively charged layers of cations with associated hydroxides and exchangeable anions in between the layers. Due to this charge balanced structure, LDHs possess the property of encapsulating an anion from the environment and replacing it with an exchangeable anion present in its layers. Potential applications include chloride entrapment in concrete and delivery of corrosion inhibiting anions. However, many versatile compositions of LDHs can be easily synthesized and their application as cement additives reach far beyond corrosion mitigation in concrete. This review presents a summary of recent advances on the applications of LDH in concrete. An extensive set of recently published literature has been critically reviewed and trends have been identified.
Reactivity of Gypsum-Based Materials Subjected to Thermal Load: Investigation of Reaction Mechanisms
Authors: Krause Felix , Renner Bernhard , Coppens Frederik , Dewanckele Jan , Schwotzer Matthias
Published: March 2020

The thermal stability of gypsum-based materials, and in this context, especially their long-term behavior, is the background of our current research activities. A comprehensive investigation program was compiled in which detailed examinations of various model materials exposed to thermal loads were carried out. The understanding of the partly not entirely consistent state of knowledge shall be sharpened especially by in situ observations of the thermally induced conversion reaction of gypsum into hemihydrate. The temporal course of the reaction was investigated non-destructively by in situ investigations in a high-resolution X-ray computed tomography setup, and the experiment was accompanied by detailed characterizations of the microstructure and composition. In this contribution, selected results of experiments with a high-purity natural gypsum rock as the model substance are presented. Studying the influence of temperature on the reaction showed that, even under supposedly dry conditions, the reaction could take place at much lower temperatures than usually reported in the literature. It was demonstrated that the transformation of gypsum into hemihydrate could take place at a temperature of already 50 °C. The results indicated that even under “classical” heating conditions in a conventional oven, the dissolution and crystallization processes in water films on the mineral surfaces could be suggested to be a driving force for the reaction. A corresponding reaction model, which took these aspects into account, was proposed in this work.
C0.3N0.7Ti-SiC Toughed Silicon Nitride Hybrids with Non-Oxide Additives Ti3SiC2
Authors: Luo Heng , Li Chen , Deng Lianwen , Li Yang , Xiao Peng , Zhang Haibin
Published: March 2020

In situ grown C0.3N0.7Ti and SiC, which derived from non-oxide additives Ti3SiC2, are proposed to densify silicon nitride (Si3N4) ceramics with enhanced mechanical performance via hot-press sintering. Remarkable increase of density from 79.20% to 95.48% could be achieved for Si3N4 ceramics with 5 vol.% Ti3SiC2 when sintered at 1600 °C. As expected, higher sintering temperature 1700 °C could further promote densification of Si3N4 ceramics filled with Ti3SiC2. The capillarity of decomposed Si from Ti3SiC2, and in situ reaction between nonstoichiometric TiCx and Si3N4 were believed to be responsible for densification of Si3N4 ceramics. An obvious enhancement of flexural strength and fracture toughness for Si3N4 with x vol.% Ti3SiC2 (x = 1~20) ceramics was observed. The maximum flexural strength of 795 MPa for Si3N4 composites with 5 vol.% Ti3SiC2 and maximum fracture toughness of 6.97 MPa·m1/2 for Si3N4 composites with 20 vol.% Ti3SiC2 are achieved via hot-press sintering at 1700 °C. Pull out of elongated Si3N4 grains, crack bridging, crack branching and crack deflection were demonstrated to dominate enhance fracture toughness of Si3N4 composites.
Amelioration of Scopolamine-Induced Amnesic, Anxiolytic and Antidepressant Effects of Ficus benghalensis in Behavioral Experimental Models
Authors: Malik Humna , Javaid Sana , Fawad Rasool Muhammad , Samad Noreen , Rizwan Ahamad Syed , Alqahtani Faleh , Imran Imran
Published: March 2020

Background and Objectives: Ficus benghalensis (FB) is a commonly found tree in Pakistan and its various parts have folkloric importance in managing neurological ailments. In the present study, methanolic extract of its bark has been tested on an experimental animal model to evaluate memory-enhancing, anxiolytic and antidepressant activities to validate the claimed therapeutic potential. Materials and Methods: Methanolic extract of freshly isolated bark was prepared and subjected to preliminary phytochemical studies and gas chromatography–mass spectrometry (GC–MS) analysis for the presence of phytocomponents. To evaluate its effect on spatial learning, passive-avoidance test–step through (PAT-ST), Y-maze and Morris water maze (MWM) tests were carried out. Open-field (OFT) and elevated plus maze (EPM) tests were employed to explore the anti-anxiety potential of FB while a forced swimming test (FST) was utilized to assess its anti-depressant prospective. FB doses of 100, 200 and 300 mg/kg with positive and negative controls given to Sprague Dawley (SD) rats. Results: phytochemical studies showed the presence of various phytoconstituents including alkaloids, flavonoids, terpenes, phenolics and anthraquinones. The presence of synephrine, aspargine, glucose, fructose and fatty acids was revealed by GC–MS analysis. FB administration led to significant improved memory retention when evaluated through passive avoidance (p < 0.05), Y-maze (p < 0.05) and Morris water maze (p < 0.05) tests in a scopolamine model of amnesic rats. When tested by open field and elevated plus maze tests, FB demonstrated anxiety-resolving characteristics (p < 0.05) as animals dared to stay in open areas more than a control group. Mobility time was increased and immobility time was reduced (p < 0.05–0.01) in rats treated with FB, unveiling the anti-depressant importance of F. benghalensis. Conclusion: methanolic extract of F. benghalensis bark furnished scientific proof behind folkloric claims of the memory improving, anxiety-reducing and depression-resolving characteristics of the plant. These activities might be possible due to interaction of its phytoconstituents with serotonergic, glutamatergic, cholinergic and GABAergic systems in the brain.
Introduction to “Chronic Rhinosinusitis and Concomitant Medical Disorders”
Authors: Gudis David A.
Published: March 2020

It is with great pleasure and enthusiasm that we present to you this Special Issue of Medical Sciences [...]
Taxonomic Characterization and Secondary Metabolite Analysis of NEAU-wh3-1: An Embleya Strain with Antitumor and Antibacterial Activity
Authors: Wang Han , Sun Tianyu , Song Wenshuai , Guo Xiaowei , Cao Peng , Xu Xi , Shen Yue , Zhao Junwei
Published: March 2020

Cancer is a serious threat to human health. With the increasing resistance to known drugs, it is still urgent to find new drugs or pro-drugs with anti-tumor effects. Natural products produced by microorganisms have played an important role in the history of drug discovery, particularly in the anticancer and anti-infective areas. The plant rhizosphere ecosystem is a rich resource for the discovery of actinomycetes with potential applications in pharmaceutical science, especially Streptomyces. We screened Streptomyces-like strains from the rhizosphere soil of wheat (Triticum aestivum L.) in Hebei province, China, and thirty-nine strains were obtained. Among them, the extracts of 14 isolates inhibited the growth of colon tumor cell line HCT-116. Strain NEAU-wh-3-1 exhibited better inhibitory activity, and its active ingredients were further studied. Then, 16S rRNA gene sequence similarity studies showed that strain NEAU-wh3-1 with high sequence similarities to Embleya scabrispora DSM 41855T (99.65%), Embleya hyalina MB891-A1T (99.45%), and Streptomyces lasii 5H-CA11T (98.62%). Moreover, multilocus sequence analysis based on the five other house-keeping genes (atpD, gyrB, rpoB, recA, and trpB) and polyphasic taxonomic approach comprising chemotaxonomic, phylogenetic, morphological, and physiological characterization indicated that the isolate should be assigned to the genus Embleya and was different from its closely related strains, therefore, it is proposed that strain NEAU-wh3-1 may be classified as representatives of a novel species of the genus Embleya. Furthermore, active substances in the fermentation broth of strain NEAU-wh-3-1 were isolated by bioassay-guided analysis and identified by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analyses. Consequently, one new Zincophorin analogue together with seven known compounds was detected. The new compound showed highest antitumor activity against three human cell lines with the 50% inhibition (IC50) values of 8.8–11.6 μg/mL and good antibacterial activity against four pathogenic bacteria, the other known compounds also exhibit certain biological activity.
Optimisation of Radium Removal from Saline Produced Waters during Oil and Gas Extraction
Authors: Garner Joel , Read David
Published: March 2020

Unconventional shale gas exploitation presents complex problems in terms of radioactive waste disposal. Large volumes of saline produced water resulting from hydraulic fracturing are typically enriched in radium isotopes, up to several hundred Bq/dm3, orders of magnitude above national discharge limits. There is a need, therefore, to decontaminate the fluid prior to discharge, preferably by creating a less problematic radium-containing, solid waste form. Barite (barium sulphate) co-precipitation is a cost-effective method for achieving these objectives, provided the process can be controlled. In this work, radium recovery of ~90% has been achieved for simulant produced waters containing 100 Bq/dm3, using a single, optimised co-precipitation step. However, salinity has a significant effect on the efficiency of the process; higher salinity solutions requiring substantially more reagent to achieve the same recovery. If >90% radium removal is sought, multiple co-precipitation steps provide a much faster alternative than post-precipitation recrystallization of the barite solid phase, albeit at higher cost. The resulting solid waste has a relatively high specific radium activity but a much smaller volume, which presents a less intractable disposal problem for site operators than large volumes of radium-contaminated fluid.
Typomorphic Features of Placer Gold from the Billyakh Tectonic Melange Zone of the Anabar Shield and Its Potential Ore Sources (Northeastern Siberian Platform)
Authors: Gerasimov Boris , Beryozkin Vasily , Kravchenko Alexander
Published: March 2020

Precambrian shields and outcropped Precambrian rock complexes in the Arctic may serve as the most important sources of various types of mineral raw materials, including gold. The gold potential of the Anabar shield in the territory of Siberia has, thus far, been poorly studied. A number of primary and placer gold occurrences have been discovered there, but criteria for the prediction of and search for gold mineralization remain unclear. The main purpose of this paper was to study the typomorphic features of placer gold in the central part of the Billyakh tectonic mélange zone in the Anabar shield and to compare them to mineralization from primary sources. To achieve this, we utilized common methods for mineralogical, petrographic, and mineragraphic analyses. Additionally, geochemical data were used. As a result of this investigation, important prospecting guides were identified, and essential criteria for the prediction of and search for gold deposits were elucidated. The characteristics of the studied placer gold were specific for gold derived from a proximal provenance. These characteristics included the poor roundness of the native gold grains, a cloddy–angular and dendritic form, an uneven surface, and a high content of coarse-fraction native gold (0.5–2 mm), which was as high as 24% of the volume of analyzed native gold. In addition, we conducted a study on the mineralogical features of the gold-sulfide mineralization that was disseminated throughout a small exposure area of paleo-Proterozoic para- and orthogneisses in the Anabar shield basement. A comparison of mineral inclusions in the coarse-fraction native gold and mineral assemblages in the ore deposits showed that one of the possible primary sources for placer gold might be small bodies of metasomatically altered orthogneisses associated with large granitoid plutons.
New Combined Depressant/Collectors System for the Separation of Powellite from Dolomite and the Interaction Mechanism
Authors: Qian Yunlou , Ding Wei , Wang Zhen , Peng Yang
Published: March 2020

The flotation beneficiation of powellite from dolomite was achieved with a new reagent system that consists of a mixed collector of sodium oleate (NaOl) and benzohydroxamic acid (BHA) and a depressant sodium hexametaphosphate (SHMP). The interaction mechanism of the reagent regime with minerals was studied using zeta potential and X-ray photoelectron spectroscopy (XPS) detection together with crystal chemistry and interaction energy analysis. The matching features of O–O distance in BHA with that in saline minerals and active site density/activity were used as methods to explain the reagent/mineral interaction. The results of microflotation finally established the new reagent regime at pH 8–12: 2.5 × 10−4 M SHMP, 2 × 10−4 M mixed collector containing 1.5 × 10−4 M NaOl and 0.5 × 10−4 M BHA. SHMP selectively depresses the adsorption of NaOl and BHA onto dolomite but minimally affects the adsorption of NaOl and BHA on the powellite surface.
Prediction Model of Aryl Hydrocarbon Receptor Activation by a Novel QSAR Approach, DeepSnap–Deep Learning
Authors: Matsuzaka Yasunari , Hosaka Takuomi , Ogaito Anna , Yoshinari Kouichi , Uesawa Yoshihiro
Published: March 2020

The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor that senses environmental exogenous and endogenous ligands or xenobiotic chemicals. In particular, exposure of the liver to environmental metabolism-disrupting chemicals contributes to the development and propagation of steatosis and hepatotoxicity. However, the mechanisms for AhR-induced hepatotoxicity and tumor propagation in the liver remain to be revealed, due to the wide variety of AhR ligands. Recently, quantitative structure–activity relationship (QSAR) analysis using deep neural network (DNN) has shown superior performance for the prediction of chemical compounds. Therefore, this study proposes a novel QSAR analysis using deep learning (DL), called the DeepSnap–DL method, to construct prediction models of chemical activation of AhR. Compared with conventional machine learning (ML) techniques, such as the random forest, XGBoost, LightGBM, and CatBoost, the proposed method achieves high-performance prediction of AhR activation. Thus, the DeepSnap–DL method may be considered a useful tool for achieving high-throughput in silico evaluation of AhR-induced hepatotoxicity.
Cytotoxic Evaluation and Anti-Angiogenic Effects of Two Furano-Sesquiterpenoids from Commiphora myrrh Resin
Authors: S. Alqahtani Ali , Nasr Fahd A. , Noman Omar M. , Farooq Muhammad , Alhawassi Tariq , Qamar Wajhul , El-Gamal Ali
Published: March 2020

Commiphora myrrh resin (Myrrh) has been used in traditional Arabic medicine to treat various inflammatory diseases. Two furano-sesquiterpenoids, 2-methoxyfuranodiene (CM1) and 2-acetoxyfuranodiene (CM2), were isolated from the chloroform fraction of the ethanolic extract of Arabic Commiphora myrrh resin. The cytotoxicity of the compounds was evaluated using human liver carcinoma, breast cancer cells (HepG2 and MCF-7, respectively) and normal human umbilical vein endothelial cells (HUVECs) cell lines. The development toxicity and anti-angiogenic activity of both compounds were also evaluated using zebrafish embryos. Cell survival assays demonstrated that both compounds were highly cytotoxic in HepG2 and MCF7 cells, with IC50 values of 3.6 and 4.4 µM, respectively. Both compounds induced apoptosis and caused cell cycle arrest in treated HepG2 cells, which was observed using flow cytometric analysis. The development toxicity in zebrafish embryos showed the chronic toxicity of both compounds. The toxicity was only seen when the embryos remained exposed to the compounds for more than three days. The compound CM2 showed a significant level of anti-angiogenic activity in transgenic zebrafish embryos at sublethal doses. Thus, we demonstrated the cytotoxic properties of both compounds, suggesting that the molecular mechanism of these compounds should be further assessed.
Evodiamine Mitigates Cellular Growth and Promotes Apoptosis by Targeting the c-Met Pathway in Prostate Cancer Cells
Authors: Hwang Sun Tae , Um Jae-Young , Chinnathambi Arunachalam , Alharbi Sulaiman Ali , Narula Acharan S. , Namjoshi Ojas A. , Blough Bruce E. , Ahn Kwang Seok
Published: March 2020

Evodiamine (EVO) is an indoloquinazoline alkaloid that exerts its various anti-oncogenic actions by blocking phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt), mitogen-activated protein kinase (MAPK), c-Met, and nuclear factor kappa B (NF-κB) signaling pathways, thus leading to apoptosis of tumor cells. We investigated the ability of EVO to affect hepatocyte growth factor (HGF)-induced c-Met/Src/STAT3 activation cascades in castration-resistant prostate cancer (CRPC). First, we noted that EVO showed cytotoxicity and anti-proliferation activities in PC-3 and DU145 cells. Next, we found that EVO markedly inhibited HGF-induced c-Met/Src/STAT3 phosphorylation and impaired the nuclear translocation of STAT3 protein. Then, we noted that EVO arrested the cell cycle, caused apoptosis, and downregulated the expression of various carcinogenic markers such as B-cell lymphoma 2 (Bcl-2), B-cell lymphoma-extra large (Bcl-xL), cyclin D1, cyclooxygenase 2 (COX-2), survivin, vascular endothelial growth factor (VEGF), and matrix metallopeptidases 9 (MMP-9). Moreover, it was observed that in cPC-3 and DU145 cells transfected with c-Met small interfering RNA (siRNA), Src/STAT3 activation was also mitigated and led to a decrease in EVO-induced apoptotic cell death. According to our results, EVO can abrogate the activation of the c-Met/Src/STAT3 signaling axis and thus plays a role as a robust suppressor of tumor cell survival, proliferation, and angiogenesis.