bluuebunny/crossref_metadata_2025 huggingface dataset:
Hipoüriseminin Muhtemel Nedeni; Ksantinüri
Authors: HÜZMELİ Can , ÖZTÜRK Kazım , GÜLLÜ Murat , CANIM Yılmaz , UÇAR Edip , CANDAN Ferhan
Published: March 2019

Kalıtımsal hipoürisemiye genellikle ksantinüri ve herediter renal hipoürisemi yol açmaktadır. Hipoürisemi serum ürik asit seviyesinin 2mg/dl’den düşük olması olarak tanımlanmaktadır. Ksantinüri her yaşta, nadir görülen ve otozomal resesif geçen bir hatalıktır. Ksantin ve hipoksantin, enzim veya kofaktör eksikliği sonucu ürik asite dönüşümü olmaz ve kanda birikir, ayrıca idrar ile atılımı artar. Serum ve idrarda ürik asit seviyesi çok düşük saptanmaktadır. Biz bu makalede,hipoürisemi saptanan ve ksantinüri tanısı konulan yetmiş yediyaşında kadınhasta litaratüreşliğinde tartışıldı.
SARC‐F Questionnaire: Rapid and Easy Tool for Identifying Physical Limitations in Hemodialysis Patients
Authors: Yamamoto Shohei , Matsuzawa Ryota , Harada Manae , Watanabe Takaaki , Shimoda Takahiro , Suzuki Yuta , Kamiya Kentaro , Osada Shiwori , Yoshida Atsushi , Matsunaga Atsuhiko
Published: March 2019

Background

SARC‐F is a simple questionnaire for rapidly assessing physical function and screening for sarcopenia. This study aimed to determine whether the SARC‐F questionnaire is useful for identifying impaired physical function and the risk of physical limitations in hemodialysis patients.

Methods and results

This cross‐sectional study included 281 patients undergoing hemodialysis at two dialysis units. SARC‐F, handgrip strength, leg strength, one‐leg standing time, usual gait speed, and short physical performance battery (SPPB) score were measured. Patients were classified according to the SARC‐F score (SARC‐F <4 and SARC‐F ≥4), indicating the risk of sarcopenia. Furthermore, we defined physical limitations as handgrip strength of <26 kg for men and <18 kg for women, leg strength of <40%, usual gait speed of ≤0.8 m/s, and SPPB score of ≤8 points. To calculate the areas under the curves (AUCs) of the SARC‐F score for physical limitations, receiver‐operating characteristic curve analysis was performed. SARC‐F ≥4 was observed in 75 (26.7 %) hemodialysis patients. The SARC‐F ≥4 group had a significantly lower handgrip and leg strength, shorter one‐leg standing time, slower usual gait speed, and lower SPPB score than the SARC‐F <4 group, even after adjusting for potential confounders (all P <0.001). Further, SARC‐F demonstrated good accuracy in identifying the risk of physical limitations (all AUCs > 0.75).

Conclusions

The SARC‐F questionnaire is useful for screening impaired physical function and for identifying the risk of physical limitations in hemodialysis patients and can be easily and rapidly administered to this patient group in clinical practice.
Evaluación de variables agronómicas, calidad del forraje y contenido de taninos condensados de la leguminosa Lotus corniculatus en respuesta a biofertilizante y fertilización química en condiciones agroecológicas de trópico alto andino colombiano
Authors: Santacoloma-Varón Luz Elena , Granados-Moreno Jairo Enrique , Aguirre-Forero Sonia Esperanza
Published: March 2019

En la sabana de Bogotá, 2600 m.s.n.m., se analizaron variables agronómicas; altura de planta, número y longitud de ramas, producción de materia seca total ha-1, y variables de calidad del forraje, digestibilidad de materia seca, proteína cruda, extracto etéreo, fibra detergente ácido, fibra detergente neutra, calcio, así como el contenido de taninos condensados de la leguminosa Lotus corniculatus, mediante un diseño completo al azar, de cuatro tratamientos aplicados al suelo T1:sin fertilizante; T2: biofertilizante; T3: fertilización química, y T4: biofertilizante más fertilización química, con tres repeticiones por tratamiento. Se utilizó análisis de varianza (ANOVA) y prueba de comparación de medias de Tukey al 0,1%. Los resultados señalan que el cultivo de L. corniculatus, responde muy bien a la biofertilización, ya que el T2 exhibió el mejor comportamiento para la mayoría de variables agronómicas y de calidad del forraje, mientras que T4 mostró los mejores resultados para número de ramas y menos contenido de taninos condensados. En digestibilidad de la materia seca T2 y T4 ostentaron un buen comportamiento con diferencias altamente significativas con respecto a T1 y T3; en contenidos de grasa y calcio, el mejor comportamiento se presentó en el T3 y T1 con diferencias altamente significativas. Se concluye que la biofertilización incide de manera favorable en las características edáficas, producción y calidad del forraje Lotus corniculatus, con mejores comportamientos en las principales variables evaluadas en el estudio.
Herramientas para evaluar la gestión de residuos hospitalarios
Authors: Betancur Diego Fernando , La Rotta Nelly Rocío , Toro Santiago , Valderrama Charry Luz Dary
Published: March 2019

Los residuos hospitalarios tienen un impacto directo sobre la salud pública y el medio ambiente puesto que son portadores de enfermedades e infecciones. El pensamiento ecológico y los nuevos parámetros de la ciencia verde obligan a implementar medidas que reduzcan el riesgo de infección y el impacto negativo en el medio ambiente. Expertos aseguran que los productos y las tecnologías utilizados en el sistema de salud han contribuido al deterioro del medio ambiente por la producción de grandes cantidades de sustancias peligrosas.

 

A pesar de que existen varios modelos y normativas para la correcta gestión de residuos hospitalarios, se hace necesario generar herramientas para la evaluación de las prácticas en torno al tratamiento de los residuos clínicos desde el momento de su generación hasta los residuos de acuerdo a los lineamientos establecidos.
Nivel de conocimientos de las enfermeras y el médico de la familia sobre la visita al hogar y los cuidados paliativos domiciliarios
Authors: Garrido Angarica Rosa Amparo , García Guanche Luís , Ordaz Hernández Niurka
Published: March 2019

En la visita al hogar se presta una atención integral, continuada y personalizada a los pacientes y su familia, es el lugar donde se alimentan, descansan, ocupan el tiempo de ocio, y se relacionan con su núcleo primario y es donde deben existir las mejores condiciones para satisfacer las necesidades biológicas, psicológicas, espirituales, sociales y culturales. Siendo necesario que las enfermeras/os y médicos de la atención primaria de salud, cuenten con las herramientas necesarias para atender tanto personas sanas como a pacientes tributarios de cuidados paliativos. Objetivo: El objetivo de este trabajo es determinar el nivel de conocimientos de las enfermeras y médicos de familia sobre la visita al hogar y los cuidados paliativos domiciliarios. Metodología: Se realizó un estudio descriptivo de corte transversal en el periodo comprendido de febrero a septiembre del año 2016, en el Policlínico Vedado. El universo estuvo formado por todos los médicos y enfermeras/os de los 40 consultorios que conforman nuestro policlínico, y te tomó una muestra de 40 enfermeras y 18 médicos a los que se les aplicó una encuesta con preguntas abiertas y cerradas. Resultados: Se constata que las enfermeras y los médicos deben trabajar aún más sobre los aspectos relacionados con al paciente y los cuidados al final de la vida. Conclusiones: Se concluye que se debe trabajar más sobre los aspectos relacionados con el paciente y los cuidados paliativos domiciliarios
La logística de reversa y su relación con la gestión integral y sostenible de residuos sólidos en sectores productivos
Authors: Peña Montoya Claudia Cecilia , Torres Lozada Patricia , Vidal Holguín Carlos Julio , Marmolejo Robellón Luis Fernando
Published: March 2019

Los residuos sólidos – RS que se generan en los sectores productivos representan fuente de oportunidades si se gestionan apropiadamente, ya que se reducen costos operativos e impactos negativos al ambiente. Este artículo presenta una reflexión acerca de la relación entre la logística de reversa – LR y la gestión integral y sostenible de residuos sólidos- GISRS en sectores productivos; relación que permite ganar eficiencia al agregar valor a los materiales con posibilidad de incorporarlos al ciclo productivo. Se recopiló y analizó información internacional y local de los desarrollos de la LR y GISRS, a través de un análisis exploratorio. El análisis realizado muestra que la LR tiene una aplicación incipiente en el sector productivo colombiano y que, aunque existe conocimiento de la temática en sectores estratégicos como el industrial, gubernamental e investigativo, es necesario el fortalecimiento de estrategias o intervenciones que permitan a los tomadores de decisiones una mejor ponderación de los beneficios económicos, sociales y ambientales asociados con su aplicación en la GISRS.
Determinando los factores del capital relacional en la interacción de cliente proveedor en MiPyMES
Authors: Álvarez-Hernández José Gerardo , Lavín-Verástegui Jesús , Pedraza-Melo Norma Angélica
Published: March 2019

El objetivo de esta investigación ha sido determinar y analizar los factores que caracterizan el capital relacional en la interacción cliente-proveedor en pequeñas y medianas empresas en una región específica de México. Con fundamento en la revisión de literatura se identifican las perspectivas de análisis de la variable de capital relacional en el trato con los proveedores. Se desarrolló y aplicó un cuestionario para recabar datos en 258 micro, pequeñas y medianas empresas (MiPyMES) del noreste de México. Se utilizó la técnica de análisis factorial exploratorio para la determinación de los factores que mejor reflejan la estructura subyacente de las variables de estudio. Se obtuvieron tres dimensiones importantes mediante lo cual se puede afirmar que la comunicación y el compromiso, la integración y la socialización son los factores que caracterizan la relación cliente - proveedor, en las micro, pequeñas y medianas empresas estudiadas.
Conformación de un panel de jueces en entrenamiento para el análisis sensorial de licores de cacao obtenidos de diferentes modelos de siembra
Authors: Quintana-Fuentes Lucas Fernando , Gómez-Castelblanco Salomón , García-Jerez Alberto , Martínez-Guerrero Nubia Consuelo
Published: March 2019

La evaluación sensorial es una disciplina de la ciencia de los alimentos que permite establecer el grado de aceptación o rechazo de un alimento por parte de los consumidores, soportado en la conformación y entrenamiento de grupos de jueces para el análisis sensorial. El objetivo principal es entrenar jueces en la evaluación sensorial de licores de cacao, contando con personal técnico de la Federación Nacional de Cacaoteros, productores y pequeños empresarios del municipio de San Vicente de Chucurí (Santander). Se realizó la selección de 18 candidatos, mediante una encuesta que permite conocer aspectos generales de salud, disponibilidad, interés, motivación y gusto por el producto. El entrenamiento se llevó a cabo en sesiones presenciales, inicialmente hubo pruebas de identificación de sabores básicos, olores y colores. Se evaluó la capacidad de los candidatos con la aplicación de pruebas de pares, dúo-trío y triangular con muestras problema. Una vez seleccionados se procedió al entrenamiento específico en aromas y sabores que se presentán en el cacao, en lo que respecta a su intensidad, calidad y calificación global, y del mismo modo, las posibles intensidades de defectos presentes, Finalmente, se comprobó el entrenamiento con la aplicación del análisis de varianza y diferencias mínimas significativas con un nivel de confianza del 0,05% para tener un grupo de jueces en entrenamiento con habilidades y competencias en el análisis de licores de cacao.
Riesgos en salud laboral asociados al uso de un bioinsecticida con esporas de Beauveria bassiana y Trichoderma lignorum
Authors: Cuervo Mulet Raúl Alberto , López Villalobos Iván Darío , Trujillo Perdomo James Frank , Fernández Daza Fabián Felipe , Vélez-Correa Sandra Lorena
Published: March 2019

El uso de bioinsecticidas para el control de plagas que atacan cultivos con efectos negativos al medio ambiente, es un tema que viene trabajándose a nivel mundial como una alternativa para evitar la contaminación los efectos sobre la salud de los trabajadores del sector agrícola. En este sentido y para controlar la hormiga arriera, se ha creado un bioinsecticida a base de esporas filamentosas de dos hongos entomopatógenos como el Beauveria bassiana y el Trichoderma lignorum. En la presente revisión sistemática de literatura se pretende identificar los efectos en la salud que pueden presentar los componentes de la formulación del bioinsecticida, entre ellos, las esporas filamentosas componente principal, los posibles efectos al medio ambiente y los beneficios socioeconómicos por su uso, hallándose datos de enfermedades como abscesos, queratitis y alergias respiratorias de las personas inmunocomprometidas. En investigaciones realizadas se identificó que las esporas filamentosas pueden ingresar al cuerpo humano por vía aérea o mucosa dado su tamaño aproximado de 0,28 µm pueden llegar hasta los alveolos pulmonares.  Ambientalmente los efectos ecológicos son positivos además por las ventajas económicas por eso de este tipo de productos agroecológicos.
La gestión crítica del conocimiento y la inteligencia colectiva y su relación con el desarrollo social
Authors: Zuluaga-Duque John Fredy
Published: March 2019

Este artículo pretende mostrar la relación existente entre la gestión crítica del conocimiento, la inteligencia colectiva y el desarrollo social. Se evidencia que un aumento en la inteligencia colectiva viene seguido de un impacto positivo en la dignificación de las personas, en el mejoramiento de las instituciones y en la sostenibilidad ambiental. En el caso de la gestión crítica del conocimiento se estudiaron las variables de producción, distribución, intercambio y consumo de los distintos saberes y conocimientos. En lo que respecta a la inteligencia colectiva se considerandoestudiaron las variables de sentido común, sensibilidad social y saberes tradicionales. Se concluye que existe una relación estrecha entre gestión critica del conocimiento e inteligencia colectiva. Se evidencia que a una mayor gestión crítica del conocimiento le sucede un mejoramiento en la capacidad de los colectivos para resolver problemas.
Marco analítico para la gobernanza territorial: la política pública de infancia y adolescencia en Colombia
Authors: Orduz Omaira , Pineda Javier A.
Published: March 2019

This article, based on a review of the concept of governance, examines the public policy of childhood and adolescence at local level in Colombia. The empirical examination of governance is carried out based on the analytical categories of management and leadership, the interaction of actors, decision spaces and multilevel structure, the design and institutional structure, the technical capacity of the levels of government and accountability. These categories show the multiple aspects that affect the configuration of local governance of this policy and understand why the guarantee of the prevailing rights of children in the territories is achieved or limited, based on two case studies.
Condiciones de empleo de un grupo de trabajadores con discapacidad en Cali, Colombia
Authors: Satizabal-Reyes Melania
Published: March 2019

People with disabilities have experienced situations of social exclusion and discrimination. However, some have managed to join the labor market. This paper aims to explore the employment conditions of a group of workers with disabilities. A quantitative, descriptive, cross-sectional study was carried out with a sample of 160 workers with disabilities in Cali. An index of employment conditions was designed. The study found that one in two workers has good employment conditions. The type of disability impacts employability. The stark diversity of the sample reveals that physically disabled workers have better employment conditions, while mentally disabled workers have worse conditions. Labor dependence influences employment conditions. Salaried workers have better employment conditions than freelance workers.
Preservice Teachers’ Discursive Constructions of Cultural Practices in a Multicultural Telecollaboration
Authors: Uzum Baburhan , Yazan Bedrettin , Avineri Netta , Akayoglu Sedat
Published: March 2019

The study reports on a telecollaboration exchange between two teacher education classes in the United States and Turkey. In synchronous and asynchronous conversations, preservice teachers (PTs) engaged in social justice issues and made discourse choices that captured culture(s) and communities as diverse or essentialized. These choices were affected by PTs’ positionings and impacted how PTs connected to individuals only and/or to broader society.  PTs asked questions that created space for critical discussions and facilitated awareness of diversity, yet sometimes led to overgeneralizations. The study has implications for designing telecollaborations that promote language and practices to unpack the issues of social justice.
The use of Napier Bones props to enhance learning achievement on Grade 5 Math multiplication
Authors: Yuriana Tri , Suwardi Suwardi
Published: March 2019

The research aims to find out the improvement of students achievement in the subject of Mathematics on the aspect of multiplication by using the teaching aid of Napier Bones. This research is conducted on the frame of class action research upon two cycles. The context of the research is at Islamic Elementary School (MI) Ma’arif Dukuh, Salatiga.  The population covers 21 of fourth grade students. The method of data population includes documentation, observation, and test. Meanwhile, the data analysis uses percentage formula. This research find out that the teaching aid of Napier Bones improve students achievement on the subject of Mathematics upon the research subjects. The significant of the result can be overviewed from the 85.71% of students that have achieved the standard of minimum completeness. It means that the teaching aid of Napier Bones is significantly proven to improve learning achievement on the aspect of multiplication.
Role of heat-shock proteins and cobalamine in maintaining methionine synthase activity.
Authors: Grabowski Michał , Banasiuk Rafał , Węgrzyn Alicja , Kędzierska Barbara , Lica Jan , Banecka-Majkutewicz Zyta , Banecki Bogdan
Published: March 2019

Atheromatous plaque is one of the most common cardiovascular-related diseases. Reports show a connection between its development and the levels of homocysteine. In pathological states high levels of homocysteine in the organism can be caused by the malfunction of the methionine synthase pathway. Bacterial methionine synthase (MetH) is a homologue of the human methionine syntase (MS). In this study we aimed to investigate the functional relations between MetH and its cofactor--cobalamine--under stress conditions. We have demonstrated that heat shock proteins (Hsp 70/100 system or HtpG) can protect MetH activity under stress conditions. Moreover, in the presence of cobalamine they can restore the activity of partially denatured methionine synthase.
Molecular factors involved in the development of diabetic foot syndrome.
Authors: Bruhn-Olszewska Bożena , Korzon-Burakowska Anna , Gabig-Cimińska Magdalena , Olszewski Paweł , Węgrzyn Alicja , Jakóbkiewicz-Banecka Joanna
Published: March 2019

Diabetes is one of the major challenges of modern medicine, as it is considered a global epidemic of the XXI century. The disease often leads to the development of serious, health threatening complications. Diabetic foot syndrome is a characteristic set of anatomical and molecular changes. At the macroscopic level, major symptoms are neuropathy, ischemia and chronic ulceration of the lower limb. In every third patient, the neuropathy develops into Charcot neuroarthropathy characterized by bone and joints deformation. Interestingly, all these complications are a result of impaired healing processes and are characteristic for diabetes. The specificity of these symptoms comes from impaired molecular mechanisms observed in type 1 and type 2 diabetes. Decreased wound and fracture healing reflect gene expression, cellular response, cell functioning and general metabolism. Here we present a comprehensive literature update on the molecular factors contributing to diabetic foot syndrome.
Effect of haloxyfop and cerulenin on de novo biosynthesis of lipids in roots of wheat and maize.
Authors: Banaś Walentyna , Furmanek Tomasz , Banaś Antoni
Published: March 2019

The study examines the effects of haloxyfop (herbicide) and cerulenin (antibiotic) on de novo biosynthesis of fatty acids and complex lipids in roots of two sensitive species: wheat and maize. Seedlings were grown in hydroponic cultures with addition of [1-(14)C]acetate (control) and [1-(14)C]acetate together with one of the tested substances. Neither haloxyfop nor cerulenin prevented the uptake of [1-(14)C]acetate by the roots of tested species. In contrast, a strong inhibition of de novo biosynthesis of fatty acids was observed after a 4-h treatment. This phenomenon, however, tended to disappear with treatment time. After a 24-h incubation, the amount of radioactivity in de novo biosynthesized fatty acids in 1-cm-long root tips was up to three times higher than in the untreated control. In the "rest of roots", restoration of fatty acid biosynthesis capacity was less pronounced. Besides the effect on fatty acid biosynthesis, both tested inhibitors strongly suppressed the de novo biosynthesis of non-fatty acid-containing lipids. Analyses of radioactivity in individual lipid classes showed that after a 4-h treatment with haloxyfop or cerulenin the biosynthesis of most of the lipid classes was inhibited, although not to the same extent. After a 24-h treatment, an inhibition of de novo biosynthesis of some of the lipids was still observable, whereas the biosynthesis of others, especially phosphatidylethanolamine and phosphatidic acid, was strongly up-regulated. Contrary to the mainstream view that inhibition of fatty acid biosynthesis is the cause of haloxyfop and cerulenin phytotoxicity, the obtained results suggest multidirectional effects of both inhibitors.
Some heterocyclic thione derivatives exhibit anticoccidial activity by inhibiting glycosidases.
Authors: Balbaa Mahmoud , Abd El-Hady Neama , Taha Nabil , El Ashry El Sayed H
Published: March 2019

Coccidiosis is one of the most common parasitic diseases affecting many species of domestic animals. This disease has a major economic significance and the search for new compounds having anticoccidial activity is of great importance. In this article, different levels of protection from coccidian infection by Eimeria stiedae were developed in rabbits by treatment with compounds incorporating the skeleton of thiourea. These compounds include 4,5-diphenylimidazole-2-thione (1), 4,5-Diphenyl-1,2,4-triazole-3-thiol (2) and 5-(2-Hydroxyphenyl)-4-phenyl-1,2,4-triazole-3-thiol (3) compared to the anticoccidial drug toltrazuril as a reference compound. Compounds 1-3 inhibit coccidiosis-induced activity of α-glucosidase. The protection from coccidial infection by compound 1 was higher than that shown for compounds 2 and 3. These data suggest that diazole and triazole thione derivatives have a mimetic effect for anticoccidial drugs through their inhibition of glycosidases.
Trace elements and rat pouchitis.
Authors: Drzymała-Czyż Sławomira , Banasiewicz Tomasz , Walas Stanisław , Kościński Tomasz , Wenska-Chyży Ewa , Drews Michał , Walkowiak Jarosław
Published: March 2019

The procedure of restorative proctocolectomy is associated with a complete removal of the colon and slight reduction of ileum length, which together can lead to systemic shortages of trace elements. Inflammatory changes in the pouch mucosa may also have some impact. However, there is no data on trace elements in pouchitis. Therefore, in the present study we aimed to assess the effect of acute pouchitis on the status of selected trace elements in rats. Restorative proctocolectomy with the construction of intestinal J-pouch was performed in twenty-four Wistar rats. Three weeks after the surgery, pouchitis was induced. Eight untreated rats created the control group. Liver concentrations of selected micronutrients (Zn, Cu, Co, Mn, Se) were measured in both groups six weeks later, using inductively coupled plasma mass spectrometry. Liver concentrations of trace elements did not differ between the study and the control groups. However, copper, cobalt and selenium concentrations [μg/g] were statistically lower (p
Differential distribution of cathepsin B in human umbilical cord tissues.
Authors: Gogiel Tomasz , Galewska Zofia , Romanowicz Lech
Published: March 2019

The extracellular matrix components are differentially distributed among various structures of the umbilical cord. Wharton's jelly is especially rich in collagens and growth factors. Cathepsin B is a major cysteine protease involved in collagen degradation, as well as in the activation of precursor forms of other collagenolytic enzymes and growth factors. We assessed the activity and expression of cathepsin B in the umbilical cord arteries, veins and Wharton's jelly. Extracts of separated umbilical cord components were subjected to an activity assay with the use of specific fluorogenic substrate. The expression of cathepsin B protein was qualitatively evaluated by Western immunoblotting and quantitatively determined with an immunoenzymatic method. The total cathepsin B activity and content calculated per gram of DNA were higher in Wharton's jelly than in the umbilical cord vessels, and the latter parameter was the lowest in the umbilical cord arteries. Moreover, the expression and the activity of latent cathepsin B (following activation by pepsin digestion) calculated per gram of DNA were the highest in Wharton's jelly and the lowest in the umbilical cord arteries. High expression and activity of latent, pepsin-activatable cathepsin B related to DNA content in Wharton's jelly seem to reflect the stimulation of its cells by high amounts of collagen I and growth factors.
Serum concentration of visfatin is decreased in patients with chronic heart failure.
Authors: Straburzyńska-Migaj Ewa , Pilaczyńska-Szcześniak Lucja , Nowak Alicja , Straburzyńska-Lupa Anna , Sliwicka Ewa , Grajek Stefan
Published: March 2019

There is an increasing interest in the role of adipocytokines in cardiovascular pathophysiology.
The aim of the study was to compare visfatin levels, a novel adipokine, in patients with heart failure (HF) due to the left ventricular systolic dysfunction with those in age- and body mass index (BMI) - matched healthy controls in relation to the parameters of glucose metabolism and high sensitivity C-reactive protein (hsCRP) levels.
The study population consisted of 28 males with systolic HF referred for cardiopulmonary exercise testing, divided into two subgroups based on their NYHA class (HF patients NYHA(I+II), n=17, and HF patients NYHA(III+IV,) n=11), and 23 controls. The following indices were measured in a serum samples: visfatin, hsCRP, glucose and lipid metabolism parameters, and the insulin resistance index HOMA(IR) (homeostasis model assessment insulin resistance) was calculated.
Concentrations of visfatin and high-density lipoprotein cholesterol (HDL-cholesterol) in the HF subjects were significantly lower (p≤0.01) than in controls. The Kruskal-Wallis test showed significant differences between three groups (controls and both subgroups of heart failure patients) in mean levels of visfatin, hsCRP, glucose, HOMA(IR) and HDL-cholesterol.
Serum visfatin concentrations in patients with systolic HF, particularly with more advanced NYHA classes, are significantly lower in comparison to healthy controls and are independent of age or anthropometric and metabolic parameters.
Properties of B16-F10 murine melanoma cells subjected to metabolic stress conditions.
Authors: Mitrus Iwona , Bryndza Ewa , Kazura Malgorzata , Smagur Andrzej , Sochanik Aleksander , Cichon Tomasz , Szala Stanislaw
Published: March 2019

Neoplastic cells which co-form tumors are usually subjected to various stress factors, mainly hypoxia and shortage of nutrient factors. Such cells employ different strategies that permit their survival under such conditions. Experiments in vitro are usually carried out in the presence of 21% oxygen and medium supplemented with 10% FBS. Altering these parameters can approximate the in vivo conditions found within tumor mass. The present paper reports certain properties (especially ability to metastasize) of B16-F10 cells able to grow upon exposure to altered growth conditions (medium supplemented with 0.06% FBS or presence of 1% oxygen for 24 or 72 hours). These properties were compared with those of control cells cultured in the presence of 21% oxygen and in medium supplemented with 10% FBS. Some properties of the cells exposed to medium supplemented with 0.06% FBS differ from those of cells cultured under low oxygenation conditions (ability to form metastases, to migrate, or to express various proteins). Only the partial deprivation of oxygen did increase both the number of migrating cells and the number of metastases formed. Serum deficiency enhanced only the cell ability to metastasize, but not to migrate. It appears that cultured B16-F10 cells employ different adaptation strategies under conditions of oxygen shortage and those of serum deficiency. Under oxygen deprivation, such cells most likely undergo an epithelial-mesenchymal transition, whereas serum deficiency ("starvation"), while increasing the tumorigenicity of B16-F10 cells, does not induce the epithelial-mesenchymal transition.
Vasostatin increases oxygenation of B16-F10 melanoma tumors and raises therapeutic efficacy of cyclophosphamide.
Authors: Cichoń Tomasz , Jarosz Magdalena , Smolarczyk Ryszard , Ogórek Barbara , Matuszczak Sybilla , Wagner Marek , Mitrus Iwona , Sochanik Aleksander , Jazowiecka-Rakus Joanna , Szala Stanisław
Published: March 2019

One of the preconditions of effective anticancer therapy is efficient transfer of the therapeutic agent (chemotherapeutic) to tumor cells. Fundamental barriers making drug delivery and action difficult include underoxygenation, elevated interstitial pressure, poor and abnormal tumor blood vascular network and acidic tumor milieu. In this study we aimed at developing an optimized scheme of administering a combination of an angiogenesis-inhibiting drug (vasostatin) and a chemotherapeutic (cyclophosphamide) in the therapeutic treatment of mice bearing experimental B16-F10 melanoma tumors. We report that the strongest tumor growth inhibition was observed in mice that received two, three or four vasostatin doses in combination with one injection of cyclophosphamide (i.e., V2 + CTX, V3 + CTX or V4 + CTX schemes). Double administration of vasostatin increases oxygenation of B16-F10 tumors. On the other hand, its five-fold administration lowers tumor oxygenation, breaks down tumor vascular network (increasing hypoxia) and leads in consequence to death of cancer cells and appearance of necrotic areas in the tumor. A decreased cyclophosphamide dose in combination with two doses of vasostatin (V2 + CTX scheme) inhibits tumor growth similarly to a larger dose of cyclophosphamide alone.
A novel, stable, helical scaffold as an alternative binder - construction of phage display libraries.
Authors: Cyranka-Czaja Anna , Otlewski Jacek
Published: March 2019

Specific, high affinity binding macromolecules are of great importance for biomedical and biotechnological applications. The most popular classical antibody-based molecules have recently been challenged by alternative scaffolds with desirable biophysical properties. Phage display technology applied to such scaffolds allows generation of potent affinity reagents by in vitro selection. Here, we report identification and characterization of a novel helical polypeptide with advantageous biophysical properties as a template for construction of phage display libraries. A three-helix bundle structure, based on Measles virus phosphoprotein P shows a very favourable stability and solubility profile. We designed, constructed and characterized six different types of phage display libraries based on the proposed template. Their functional size of over 10(9) independent clones, balanced codon bias and decent display level are key parameters attesting to the quality and utility of the libraries. The new libraries are a promising tool for isolation of high affinity binders based on a small helical scaffold which could become a convenient alternative to antibodies.
Purification and characterization of a novel laccase from the mushroom Pleurotus nebrodensis.
Authors: Tian Guo-Ting , Zhang Guo-Qing , Wang He-Xiang , Ng Tzi Bun
Published: March 2019

A novel laccase with a molecular mass of 64 kDa and the N-terminal sequence AIGPDDTINF was isolated from fresh fruiting bodies of the mushroom Pleurotus nebrodensis. The purification protocol comprised ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-Sepharose, and gel filtration on Superdex 75. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose, but not on CM-cellulose. It demonstrated an optimal temperature of 70°C. The enzyme activity increased steadily over the temperature range 20°C-70°C. There was only a slight reduction in activity at 80°C. However, all activity disappeared following exposure to 100°C for 10 minutes. The enzyme activity changed only slightly over the pH range 3-5, with the optimum at pH 5, but underwent a precipitous decline when the pH was elevated to 6, and was undetectable at pH 8 and pH 9.
In vitro production of M. × piperita not containing pulegone and menthofuran.
Authors: Bertoli Alessandra , Leonardi Michele , Krzyzanowska Justyna , Oleszek Wieslaw , Pistelli Luisa
Published: March 2019

The essential oils (EOs) and static headspaces (HSs) of in vitro plantlets and callus of Mentha x piperita were characterized by GC-MS analysis. Leaves were used as explants to induce in vitro plant material. The EO yields of the in vitro biomass were much lower (0.1% v/w) than those of the parent plants (2% v/w). Many typical mint volatiles were emitted by the in vitro production, but the callus and in vitro plantelet EOs were characterized by the lack of both pulegone and menthofuran. This was an important difference between in vitro and in vivo plant material as huge amounts of pulegone and menthofuran may jeopardise the safety of mint essential oil. Regarding the other characteristic volatiles, menthone was present in reduced amounts (2%) in the in vitro plantlets and was not detected in the callus, even if it represented the main constituent of the stem and leaf EOs obtained from the cultivated mint (26% leaves; 33% stems). The M. piperita callus was characterized by menthol (9%) and menthone (2%), while the in vitro plantlet EO showed lower amounts of both these compounds in favour of piperitenone oxide (45%). Therefore, the established callus and in vitro plantlets showed peculiar aromatic profiles characterized by the lack of pulegone and menthofuran which have to be monitored in the mint oil for their toxicity.
Molecular Docking Study of Four Chromene Derivatives as Novel HIV-1 Integrase Inhibitors
Authors: ARSLAN Nevin
Published: March 2019

Four ligands based on chromene derivatives have been docked into integrase of prototype foamy virus, which has a quite similar structural similarity with that of HIV-1 integrase using Autodock Vina (Vina). The docking scores for the derivatives are -7.3 kcal/mol, -7.5 kcal/mol, -6.9 kcal/mol, and -7.2 kcal/mol, respectively, which are comparable with that for Raltegravir (-10.7 kcal/mol). The docking results provide a detailed evidence for the interactions of four chromene derivatives. The results may lead to the design and development of new drug candidates against AIDS
Online programming language—Learning management system
Authors: Salama Ramiz , Qazi Ahmed , Elsayed Mohamed
Published: March 2019

Today, college and university students find themselves with the need more than getting a degree. Work and social life commitments make equal demands on their time. The option of taking online classes and studying on their own is a must. However, many state institutions are unable to accommodate all those who want to take classes on campus, escalating the demand for online learning. The aim of this project is development of learning management system (LMS). You can be as a teacher and upload your course materials to all students in our LMS. With our LMS, you are online every time, you can get messages from all students and teacher, without e-mail, just by your username in our system. Also, you can compile your code and share the result with others by social media links using REPL IT online compiler. So, our LMS provide all facilities for taking online course. Keywords: LMS, compiler, cloud EC2, route 53, easy engine, learn press.
Mobile accident report and road assistance application
Authors: Solak Nadide Duygu , Topaloglu Murat
Published: March 2019

The number of mobile applications has been increasing rapidly in every field of life with the increasing use of smart devices. Smartphones and tablets make our lives easier with their properties and application they include. Minor or major accidents in traffic are always present in the daily life resulting in financial damage and loss of lives. There have been a number of studies done to speed up the processes to be done from the moment an accident happens. This study aims to enable people to perform all of the post-accident processes quickly and accurately with the use of mobile devices. In this way, papers and documents like photographs will be sent to the competent authorities without wasting time and effort. In addition, access to the road assistance needed will be quite easy. Keywords: Traffic accident, loss assessment and proceedings, mobile application.
Revisión documental de uso de los aceites esenciales obtenidos de Lippia alba (Verbenaceae), como alternativa antibacteriana y antifúngica
Authors: Acero-Godoy Jovanna , Guzmán-Hernández Tomas , Muñoz-Ruíz Carlos
Published: March 2019

Los antibióticos, son compuestos químicos que se utilizan para erradicar algunos microorganismos patógenos, sin embargo según informes de la OMS, se ha desarrollado resistencia a los antibióticos por parte de algunos microorganismos de importancia clínica; lo cual ha limitado las opciones de tratamiento por parte de los profesionales de la salud a los pacientes. Por lo anterior, se ha visto la necesidad de buscar terapias alternativas como el uso de AE (Aceites Esenciales) de plantas aromáticas como una biosolución a los tratamientos convencionales con muy buenos resultados. Los AE, obtenidos por hidrodestilación de la planta Lippia alba, la cual está distribuida ampliamente en Latinoamérica han presentado resultados promisorios por la gran variedad de compuestos volátiles como citral, carvona, mirceno entre los más abundantes y otros 30 compuestos aproximadamente; la variación en la concentración de los compuestos depende de diversos factores fenotípicos y genotípicos, así como ambientales; diferentes investigaciones han demostrado como la extracción del aceite de las hojas y su uso tanto in vivo como in vitro han presentado actividad bactericida, bacteriostática, antifúngica, antiprotozoal, antitumoral, como sedante para el transporte de animales, como promotor de crecimiento animal, entre otros; esta revisión documental muestra algunas investigaciones realizadas con el aceite esencial de L. alba y los resultados obtenidos en las mismas.
Potencia Reactiva: Despacho Óptimo con una programación lineal-PARTE A
Authors: Gómez-Ramírez Gustavo Adolfo
Published: March 2019

La toma de decisiones se basa en aspectos técnicos, legales, pero sobretodo económico, no obstante, se requiere de gran experiencia del negocio para de esta manera ser acertado y tratar de no impactar negativamente. En el siguiente trabajo de investigación parte A, plantea un despacho óptimo de potencia reactiva a partir de la utilización de una optimización por programación lineal; dicha potencia es de gran importancia en los Sistemas Eléctricos de Potencia para el control y regulación de los perfiles de tensión. De igual forma contribuye en el flujo de potencia real de las líneas de transmisión. Por otro lado, es importante señalar, que los sistemas eléctricos a nivel mundial, no cuantifican su uso ni establecen valores para su cobro, por lo que el aporte de esta investigación va más allá de utilizar una herramienta de fácil uso, sino de proponer estructuras tarifarias donde se incluyan costos por el uso de la potencia reactiva y de esta manera disminuir los precios de la electricidad. Por último, en el presente trabajo se desarrolla en un sistema de potencia tipo IEEE-14.
Establecimiento de un protocolo de transformación transitoria y estable de Nicotiana tabacum mediada por Agrobacterium tumefaciens
Authors: Méndez-Muñoz Laura , Campos-Delgado So a , Obando-Coronado Mariel , Garro-Monge Giovanni
Published: March 2019

Actualmente las herramientas biotecnológicas como la ingeniería genética y la biología molecular se encuentran muy avanzadas, permitiendo el desarrollo de sistemas que se basan en el uso de células vegetales para la producción de proteínas recombinantes, lo cual permite el desarrollo de plataformas para la elaboración de biofármacos, enzimas y metabolitos secundarios. El uso de metodologías para transformación transitoria y estable, permiten la producción de proteínas recombinantes de interés. En este trabajo se realizó una transformación estable de callo de N. tabacum de la línea comercial BY-2 y una silvestre (T1). Además se realizaron ensayos de agroinfiltración por la técnica al vacío y con la técnica por jeringa. En ambos casos, la transformación fue mediada por Agrobacterium tumefaciens LBA4404 la cual poseía el vector pCAMBIA 1303, conteniendo los genes marcadores GFP y GUS, y un gen de resistencia a Higromicina. Se evaluó la expresión de la proteína verde fluorescente por medio de microscopía de fluorescencia. La expresión de los genes de resistencia a Higromicina y β-glucoronidasa se evaluaron por medio de PCR convencional. Se obtuvieron porcentajes de transformación aceptables, en conjunto con una alta expresión de la proteína verde fluorescente.
Extracción de nutrientes, crecimiento y producción del cultivo de Pepino bajo sistema de cultivo protegido hidropónico
Authors: Ramírez-Vargas Carlos
Published: March 2019

Se sembraron tres genotipos comerciales de pepino (Cucumis sativus L.), Modan RZ, Tropicuke y Monalisa, en ambiente protegido con sistema hidropónico abierto en San Carlos, Costa Rica. Las plantas se trasplantaron en contenedores plásticos con sustrato de fibra de coco provisto de un sistema de fertirriego y tutorado, se realizaron mediciones de crecimiento, producción, y absorción de nutrientes. Los niveles de absorción de nutrientes, crecimiento y producción fueron diferentes entre genotipos. El orden de absorción de macronutrientes en todos los cultivares fue K>N>Ca>Mg>P, y de micronutrientes fue Fe>Zn>Mn>Cu.

Los rangos de crecimiento de los genotipos fueron de 270.03 a 287.92 y 221.72 a 278.28 cm de altura máxima, 61.86 a 82.83 y 18.27 a 20.75 hojas por planta, 432.98 a 562.83 y 148.88 a 202.47 cm2 en área foliar, 6.41 a 8.34 y 2.21 a 4.16 de índice de área foliar, 294.86 a 349.70 y 174.94 a 206.50 g de peso fresco vegetativo (parte aérea + raíz), 342.60 a 361.97 y 203.67 a 274.40 g de peso fresco de fruto, 21.38 a 27.76 y 13.55 a 15.38 g de peso seco vegetativo, 5.53 a 11.06 y 4.43 a 7.92 g de peso seco de fruto respectivamente. El rendimiento en el cv Modán RZ fue superior con un total de 2895.31 g/planta, y el menor fue de 2001 g/planta.
Tibial Diaphyseal Fracture Rates Following Tibial Tuberosity Advancement Rapid (TTAR) and Traditional Tibial Tuberosity Advancement (tTTA)
Authors: Chong Wye Li
Published: March 2019

PICO question

In dogs with cruciate disease, is the use of TTA Rapid (TTAR) compared to traditional TTA (tTTA) associated with a higher risk of tibial diaphyseal fractures?
Clinical bottom line

Based on studies published between January 2013 and January 2018, the rate of tibial diaphyseal fractures as a complication of Tibial Tuberosity Advancement Rapid (TTAR) surgery is within the published limits of traditional Tibial Tuberosity Advancement (tTTA). In this period, seven studies were related to tTTA, comprising of four; one case series, two retrospective case-control studies, and one retrospective cohort study. Five case series were related to TTAR.  All evidence within this period has been observational (Level 3 and 4 evidence). No direct head-to-head comparison between the techniques has been studied. 

  
PENYALAHGUNAAN KEADAAN (MISBRUIK VAN OMSTANDIGHEDEN) SEBAGAI LARANGAN DALAM PERJANJIAN SYARIAH
Authors: Fidhayanti Dwi
Published: March 2019

The principle of freedom of contract may cause problems in the form of an indication of misuse of circumstances (misbruik van omstandigheden) in the agreement. Misuse of circumstances (misbruik van omstandigheden) occurs when a person in a covenant is influenced by something that prevents him from making a judgment free from the other so that he can not take an independent decision. This research uses normative research with a legislative approach. invitations and conceptual approaches. Legal material is obtained through literature study and analyzed by legal interpretation method. The results of the study and discussion show that the concept of misuse of state (misbruik van omstandigheden) in the agreement is included in a covenant with a defective will. One party who has a strong position to suppress and even threaten against parties who have a weaker position so Bargaining position is not balanced. Persons who have a weak position are not given the freedom to give their opinion on the contents of the agreement. Sharia treaties that contain elements of abuse of the circumstances (misbruik van omstandigheiden or undue influence) are included in the forbidden agreement, called ikrah. To do slander is to do wrongdoing. As a result of the law, the agreement becomes null and void.
Asas kebebasan berkontrak dapat menimbulkan permasalahan berupa adanya indikasi penyalahgunaan keadaan (misbruik van omstandigheden) dalam perjanjian. Penyalahgunaan keadaan (misbruik van omstandigheden) terjadi manakala seseorang di dalam suatu perjanjian dipengaruhi oleh suatu hal yang menghalanginya untuk melakukan penilaian (judgement) yang bebas dari pihak lainnya, sehingga ia tidak dapat mengambil putusan yang independen.Penelitian ini menggunakan penelitian normatif  dengan pendekatan perundang-undangan dan pendekatan konseptual. Bahan hukum didapatkan melalui studi pustaka dan dianalisis dengan metode intepretasi hukum. Hasil penelitian dan pembahasan menunjukkan bahwa konsep penyalahgunaan keadaan (misbruik van omstandigheden) dalam perjanjian termasuk dalam perjanjian yang cacat kehendak. Salah satu pihak yang mempunyai posisi kuat untuk menekan bahkan mengancam terhadap pihak yang mempunyai posisi lebih lemah sehingga Bargaining position tidak seimbang. Pihak yang punya posisi lemah tidak diberikan kebebasan untuk memberikan pendapatnya atas isi perjanjian. Perjanjian syariah yang mengandung unsur penyalahgunaan keadaan (misbruik van omstandigheiden atau undue influence) termasuk pada perjanjian yang dilarang, yang disebut dengan ikrah. Berbuat Ikrah berarti berbuat zalim. Akibat hukumnya, yaitu perjanjian tersebut menjadi batal demi hukum.
How the Incorrect Belief That Eating Disorders Are Predominantly Genetic Is Maintained
Authors: Ross Colin A.
Published: March 2019

The incorrect belief that anorexia nervosa is predominantly genetic is maintained in the psychiatric literature by a series of misquotations and misrepresentations of research data. An example of this type of scholarship is as an editorial in The American Journal of Psychiatry. Data from family and twin studies referenced in the editorial provide compelling evidence that the genetic contribution to the etiology of anorexia nervosa is small. The incorrect belief that anorexia nervosa is predominantly genetic is maintained, in addition, by statistical procedures such as heritability estimates. The incorrect belief that anorexia nervosa is predominantly genetic should not be endorsed by the American Psychiatric Association, in either its journals, in its published books, or in DSM–V.