bluuebunny/crossref_metadata_2025 huggingface dataset:
Surgical Treatment of Sacral Metastatic Tumors
Authors: Sun Mengxiong , Zuo Dongqing , Wang Hongsheng , Sheng Jiakang , Ma Xiaojun , Wang Chongren , Zan Pengfei , Hua Yingqi , Sun Wei , Cai Zhengdong
Published: June 2021

Objective

This study intends to retrospectively analyze the data of patients with sacral metastases in our center, and analyze the treatment methods and therapeutic effects of sacral metastases.

Methods

73 patients with sacral metastases treated in our hospital from June 2013 to June 2019 were retrospectively analyzed. There were 54 cases of neurological symptoms, 42 cases of sacroiliac joint instability, 24 cases of lower limb muscle weakness and 19 cases of abnormal urination and defecation. Four patients with tumors below S3 underwent complete tumor resection, 23 patients with tumors above S3 and without sacroiliac joint instability underwent tumor curettage and nerve root lysis, 34 patients with tumors above S3 and sacroiliac joint instability underwent tumor curettage, nerve root release and screw rod reconstruction. 12 patients with multiple metastases underwent percutaneous radiofrequency ablation and sacroplasty. VAS was used to evaluate the preoperative and postoperative pain scores, and the postoperative pain relief, neurological function, bowel function, wound healing and complications were evaluated.

Results

There were no perioperative death, 8 cases of poor wound healing, 5 cases of nerve injury, postoperative sensory and motor loss of lower limbs. Cerebrospinal fluid (CSF) leak in 7 cases. The patients were followed up for 6-25 months (mean 12 months). The VAS scores of patients with pain symptoms were 7 points before operation and 1.44 points after operation, In 19 patients with abnormal urination and defecation function, 12 patients recovered to normal 3-6 months after operation, 5 cases had no significant change compared with preoperative, and 2 cases had aggravated symptoms; 17 cases of patients with lower limb muscle strength were significantly recovered after operation, and the average muscle strength was increased by 2 grades; 30 cases of patients with unstable sacroiliac joint got internal fixation had significantly pain relief. Pain symptoms of 9 patients were significantly relieved after percutaneous radiofrequency ablation.

Conclusion

the operation of sacral metastases mainly adopts a relatively conservative surgical method, which can effectively improve the quality of life of patients with sacral metastases by retaining the nerve function and relieving the pain of patients, combining with radiofrequency ablation, sacroplasty and targeted drugs.
The Case Selection for Vaginal Cuff Brachytherapy in Cervical Cancer Patients After Radical Hysterectomy and External Beam Radiation Therapy
Authors: Lai Ying-Lu , Jin Ye-Ning , Wang Xi , Qi Wei-Xiang , Cai Rong , Xu Hao-Ping
Published: June 2021

Objective

To explore the suitable cases for vaginal cuff brachytherapy (VCB) combined with external beam radiation therapy (EBRT) in the postoperative treatment of cervical cancer.

Methods

We retrospectively analyzed the clinical data of 214 postoperative cervical cancer patients who received radiotherapy from January 2008 to December 2015. Among them, 146 patients received postoperative EBRT, 68 received EBRT plus VCB. There was no statistical difference in clinical and pathological characteristics between these two groups. Those who with negative vaginal cuff underwent supplemented 12–18 Gy/2–3 Fx VCB. Survival analyses were performed using Kaplan–Meier method, and Cox model was used to analyze prognostic factors.

Results

The median follow-up was 52 months (9–136 months), and 4-year RFS (recurrence-free survival) was 77%. Among them, 58 patients (27.10%) had local or distant recurrences, 29 (13.55%) in pelvis, six (2.80%) with metastases to para-aortic, 19 (8.88%) with distant metastases (including inguinal lymph nodes) and four (1.87%) with both local and distant recurrences. The postoperative brachytherapy boost did not improve RFS or OS (overall survival) among the investigated subjects, P = 0.77, P = 0.99, respectively. Neither it decreased the local relapse in the pelvis or vaginal cuff, P = 0.56, P = 0.59. Subgroup analyses showed that brachytherapy boost improved RFS in patients who had bulky mass (>4 cm) as well as 1) with deep stromal invasion (>50% stromal invasion), P = 0.012 or 2) received low EBRT dose (≤45 Gy), P = 0.033, and in patients with deep stromal invasion as well as received low EBRT dose (P = 0.018).

Conclusions

We first proposed the case selection model for postoperative EBRT plus VCB. Brachytherapy boost were considered in the setting of postoperative radiotherapy if the patients had at least two out of these following factors: bulky mass, deep stromal invasion and low EBRT dose.
Predictive Value of Skeletal Muscle Mass in Recurrent/Metastatic Head and Neck Squamous Cell Carcinoma Patients Treated With Immune Checkpoint Inhibitors
Authors: Arribas Lorena , Plana Maria , Taberna Miren , Sospedra Maria , Vilariño Noelia , Oliva Marc , Pallarés Natalia , González Tampán Ana Regina , Del Rio Luis Miguel , Mesia Ricard , Baracos Vickie
Published: June 2021

Background

Reduced muscle mass has been associated with increased treatment complications in several tumor types. We evaluated the impact of skeletal muscle index (SMI) on prognosis and immune-related adverse events (IrAEs) in a cohort of recurrent/metastatic (R/M) head and neck squamous cell carcinoma (HNSCC) treated with immune checkpoints inhibitors (ICI).

Methods

A single-institutional, retrospective study was performed including 61 consecutive patients of R/M HNSCC diagnosed between July 2015 and December 2018. SMI was quantified using a CT scan at L3 to evaluate body composition. Median baseline SMI was used to dichotomize patients in low and high SMI. Kaplan-Meier estimations were used to detect overall survival (OS) and progression-free survival (PFS). Toxicity was recorded using Common Terminology Criteria for Adverse Event v4.3.

Results

Patients were 52 men (85.2%) with mean of age 57.7 years (SD 9.62), mainly oral cavity (n = 21; 34.4%). Low SMI was an independent factor for OS in the univariate (HR, 2.06; 95% CI, 1.14–3.73, p = 0.017) and multivariate Cox analyses (HR, 2.99; 95% CI, 1.29–6.94; p = 0.011). PFS was also reduced in patients with low SMI (PFS HR, 1.84; 95% CI, 1.08–3.12; p = 0.025). IrAEs occurred in 29 (47.5%) patients. There was no association between low SMI and IrAEs at any grade (OR, 0.56; 95% CI, 0.20–1.54; p = 0.261). However, grades 3 to 4 IrAEs were developed in seven patients of whom three had low SMI.

Conclusions

Low SMI before ICI treatment in R/M HNSCC patients had a negative impact on OS and PFS. Further prospective research is needed to confirm the role of body composition as a predictive biomarker in ICI treatment.
Metabolic and Network Pharmacological Analyses of the Therapeutic Effect of Grona styracifolia on Calcium Oxalate-Induced Renal Injury
Authors: Chen Wei , Si Yachen , Cheng Jin , Ding Jiarong , Zhao Hongxia , Liu Wenrui , Lin Qishan , Hou Jiebin , Guo Zhiyong
Published: June 2021

Grona styracifolia (Osbeck) Merr. (GS), a popular folk medicine, is clinically applied to treat nephrolithiasis. In this study, a urinary metabolic analysis was performed in a mouse model of renal calcium oxalate (CaOx) crystal deposition to identify the differentially altered metabolites in mice with oxalate-induced renal injury and explore the therapeutic mechanisms of GS against nephrolithiasis. Twenty-four mice were randomly divided into the control, oxalate and GS-treated groups. A metabolomics approach based on ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used to analyze the metabolic profiles of the urine samples. In addition, network pharmacology analysis was performed with different databases. As a result, the protective effects of GS were verified by measuring biochemical parameters and detecting crystal deposition. Fifteen metabolites were identified as the differentially altered metabolites in mice with crystal-induced renal injury. Most were involved in amino acid and fatty acid metabolism. Thirteen of these metabolites showed a reversal trend following GS treatment. A component-target-metabolite network was further constructed and nine overlapping target proteins of GS and the differentially altered metabolites were discovered. Among these proteins, the expression of estrogen receptor 2 (ESR2) in renal tissues was significantly down-regulated while androgen receptor (AR) expression was obviously increased in the oxalate group compared with the control group. These changes were reversed by the GS treatment. In conclusion, GS exerts its therapeutic effect by regulating multiple metabolic pathways and the expression of ESR and AR in mice with oxalate-induced renal injury.
Systems Pharmacology–Based Dissection of Anti-Cancer Mechanism of Traditional Chinese Herb Saussurea involucrata
Authors: Zhang Qian , He Lanyu , Jiang Qingqing , Zhu Hongqing , Kong Dehua , Zhang Hua , Cheng Zhiqiang , Deng Hongtao , Zheng Yaxin , Ying Xue
Published: June 2021

Cancer has the highest mortality in humans worldwide, and the development of effective drugs remains a key issue. Traditional Chinese medicine Saussurea involucrata (SI) exhibits a series of effects, such as anti-cancer, but the action mechanisms are still unclear. Here, systems pharmacology was applied to reveal its anti-cancer mechanism. First, we screened the active compounds of SI. Then, the compound–target network, target–disease network, and target–pathway network were constructed. DAVID was applied for GOBP analysis and KEGG pathway enrichment analysis on cancer-related targets. Seven potential compounds and 187 targets were identified. The target–disease classification network showed that compounds mainly regulated proteins related to cancer, nervous system diseases, and cardiovascular system diseases. Also, SI anti-tumor effect mainly associated with the regulation of NO production, angiogenesis, MAPK, and PKB from GOBP enrichment. Additionally, KEGG pathway enrichment indicated that targets involved in anti-inflammatory action, inhibiting angiogenesis and anti-proliferation or inducing apoptosis. Experimental validation showed that four active compounds could inhibit cell proliferation and promote apoptosis in A549 (except for kaempferol), PC-3, and C6 cells. This study not only provides experimental evidence for further research on SI in cancer treatment but also promotes the development of potential drugs of SI in modern medicine.
Structural Characterization and Immunomodulatory Activity of a Novel Polysaccharide From Lycopi Herba
Authors: Zhang Wuxia , Hu Yihua , He Jiaqi , Guo Dongdong , Zhao Jinzhong , Li Peng
Published: June 2021

Lycopi Herba has been broadly used as a traditional medicinal herb in Asia due to its ability to strengthen immunity. However, it is still obscure for its material basis and underlying mechanisms. Polysaccharide, as one of the most important components of most natural herbs, usually contributes to the immunomodulatory ability of herbs. Here, we aimed to detect polysaccharides from Lycopi Herba and examine their potential immunomodulatory activity. A novel polysaccharide (LHPW) was extracted from Lycopi Herba and purified by DEAE-52 cellulose chromatography and G-100 sephadex. According to physicochemical methods and monosaccharide composition analysis, LHPW was mainly composed of galactose, glucose, fructose, and arabinose. NMR and methylation analyses indicated that LHPW was a neutral polysaccharide with a backbone containing →3,6)-β-D-Galp-(1→, →4)-β-D-Galp-(1→ and →4)-α-D-Glcp-(1→, with the branches of →1)-β-D-Fruf-(2→ and →6)-α-D-Galp-(1→. Immunological tests indicated that LHPW could activate macrophage RAW264.7 and promote splenocyte proliferation. This study discovered a novel polysaccharide from Lycopi Herba and showed it was a potential immunomodulator.
Modeling NO Biotransport in Brain Using a Space-Fractional Reaction-Diffusion Equation
Authors: Tamis Andrew , Drapaca Corina S.
Published: June 2021

Nitric oxide (NO) is a small gaseous molecule that is involved in some critical biochemical processes in the body such as the regulation of cerebral blood flow and pressure. Infection and inflammatory processes such as those caused by COVID-19 produce a disequilibrium in the NO bioavailability and/or a delay in the interactions of NO with other molecules contributing to the onset and evolution of cardiocerebrovascular diseases. A link between the SARS-CoV-2 virus and NO is introduced. Recent experimental observations of intracellular transport of metabolites in the brain and the NO trapping inside endothelial microparticles (EMPs) suggest the possibility of anomalous diffusion of NO, which may be enhanced by disease processes. A novel space-fractional reaction-diffusion equation to model NO biotransport in the brain is further proposed. The model incorporates the production of NO by synthesis in neurons and by mechanotransduction in the endothelial cells, and the loss of NO due to its reaction with superoxide and interaction with hemoglobin. The anomalous diffusion is modeled using a generalized Fick’s law that involves spatial fractional order derivatives. The predictive ability of the proposed model is investigated through numerical simulations. The implications of the methodology for COVID-19 outlined in the section “Discussion” are purely exploratory.
Does Attentional Focus Influence Psychophysiological Responses to an Acute Bout of Exercise? Evidence From an Experimental Study Using a Repeated-Measures Design
Authors: Meixner Friedrich , Herbert Cornelia
Published: June 2021

Attentional focus during aerobic exercise has been studied in the context of sports performance, injury prevention and affective experience. Previous research suggests that an additional mental task parallel to the physical activity might influence exercise experience and performance. It has been tested if attentional focus influences cardiovascular activity, positive/negative affect, and subjective exertion during a cycling exercise. Data from N = 30 female participants has been collected using a repeated measures design, with the following experimental manipulations: (A) an internal attention focus (i.e., paying attention to force production of the quadriceps muscles), (B) an external attention focus (i.e., paying attention to changes in brightness in the cycling track simulation), and as control conditions, (C) exercise without attention focus (i.e., no specific instruction was given) and (D) no exercise, no attention focus. Subjective affect and subjective exertion were assessed, and changes in cardiovascular activity were recorded via mobile impedance cardiography (ICG) at rest, during and after the exercise, including HR, HRV (RMSSD, HF), PEP, CO, SV, LVET, and RSA. Exercise was associated with adaptations in cardiovascular activity, positive/negative affect, and subjective exertion. However, this did not interact with attentional focus. The original hypothesis could not be supported: instructed attentional focus does not influence affect, exertion, or cardiovascular activity during a cycling exercise. Therefore, attentional focusing during exercise does not appear to put notable additional mental demands on the physically active participant. Nonetheless, impedance cardiography delivered reliable measurements even during the cycling exercise.
Dietary Supplementation of Baicalein Affects Gene Expression in Broiler Adipose Tissue During the First Week Post-hatch
Authors: Xiao Yang , Halter Bailey , Boyer Casey , Cline Mark A. , Liu Dongmin , Gilbert Elizabeth R.
Published: June 2021

Dietary supplementation of baicalein, a flavonoid, has anti-obesity effects in mammals and broiler chickens. The aim of this study was to determine the effect of dietary baicalein supplementation on broiler growth and adipose tissue and breast muscle deposition. Fifty Hubbard × Cobb-500 day-of-hatch broiler chicks were assigned to a control starter diet or control diet supplemented with 125, 250, or 500 mg/kg baicalein and diets were fed for the first 6 days post-hatch. Body weight, average daily body weight gain, and average daily food intake were all reduced by 500 mg/kg baicalein. Breast muscle and subcutaneous and abdominal fat weights were also reduced in chicks that consumed the baicalein-supplemented diets. mRNAs for genes encoding factors involved in adipogenesis and fat storage, 1-acylglycerol-3-phosphate-O-acyltransferase 2, CCAAT/enhancer-binding protein β, perilipin-1, and sterol regulatory element-binding transcription factor 1, were more highly expressed in the adipose tissue of broilers supplemented with baicalein than the controls, independent of depot. Diacylglycerol acyltransferase and peroxisome proliferator-activated receptor gamma mRNAs, involved in triacylglycerol synthesis and adipogenesis, respectively, were greater in subcutaneous than abdominal fat, which may contribute to differences in expansion rates of these depots. Results demonstrate effects of dietary supplementation of baicalein on growth performance in broilers during the early post-hatch stage and molecular effects in major adipose tissue depots. The mild reduction in food intake coupled to slowed rate of breast muscle and adipose tissue accumulation may serve as a strategy to modulate broiler growth and body composition to prevent metabolic and skeletal disorders later in life.
Hepatic Branch Vagotomy Modulates the Gut-Liver-Brain Axis in Murine Cirrhosis
Authors: Zhang Yuan , Kang Jason D. , Zhao Derrick , Ghosh Siddartha S. , Wang Yanyan , Tai Yunling , Gonzalez-Maeso Javier , Sikaroodi Masoumeh , Gillevet Patrick M. , Lippman H. Robert , Hylemon Phillip B. , Zhou Huiping , Bajaj Jasmohan S.
Published: June 2021

Background

Cirrhosis and hepatic encephalopathy (HE) are linked with an altered gut-liver-brain axis, however, the relative contribution of hepatic vagal innervation is unclear. We aimed to determine the impact of hepatic vagotomy on the gut microbiome, brain, and liver in murine cirrhosis.

Methods

10–15-week-old male C57BL/6 mice with and without hepatic vagotomy underwent carbon tetrachloride (CCl4) gavage for 8 weeks. Frontal cortex [inflammation, glial/microglial activation, BDNF (brain-derived neurotrophic factor)], liver [histology including inflammation and steatosis, fatty acid synthesis (sterol-responsive binding protein-1) SREBP-1, insulin-induced gene-2 (Insig2) and BDNF], and colonic mucosal microbiota (16srRNA microbial sequencing) were evaluated on sacrifice. Conventional mice with and without cirrhosis were compared to vagotomized counterparts.

Results

Conventional control vs. cirrhosis: Cirrhosis resulted in dysbiosis, hepatic/neuro-inflammation with glial/microglial activation, and low brain BDNF vs. controls. Conventional control vs. vagotomy controls: Vagotomized control mice had a lower colonic dysbiosis than conventional mice but the rest of the hepatic/brain parameters were similar. Conventional cirrhosis vs. vagotomized cirrhosis: After vagotomy + cirrhosis, we found lower dysbiosis but continuing neuroinflammation in the absence of glial/microglial activation vs. conventional cirrhosis. Vagotomy + Cirrhosis groups showed higher hepatic steatosis due to higher SREBP1 and low Insig2 protein and altered activation of key genes involved in hepatic lipid metabolism and inflammation. BDNF levels in the brain were higher but low in the liver in vagotomy + cirrhosis, likely a protective mechanism.

Conclusions

Hepatic vagal innervation affects the gut microbial composition, hepatic inflammation and steatosis, and cortical inflammation and BDNF expression and could be a critical modulator of the gut-liver-brain axis with consequences for HE development.
Phloretin, an Apple Phytoalexin, Affects the Virulence and Fitness of Pectobacterium brasiliense by Interfering With Quorum-Sensing
Authors: Pun Manoj , Khazanov Netaly , Galsurker Ortal , Weitman Michal , Kerem Zohar , Senderowitz Hanoch , Yedidia Iris
Published: June 2021

The effects of phloretin a phytoalexin from apple, was tested on Pectobacterium brasiliense (Pb1692), an emerging soft-rot pathogen of potato. Exposure of Pb1692 to 0.2 mM phloretin a concentration that does not affect growth, or to 0.4 mM a 50% growth inhibiting concentration (50% MIC), reduced motility, biofilm formation, secretion of plant cell wall-degrading enzymes, production of acyl–homoserine lactone (AHL) signaling molecules and infection, phenotypes that are associated with bacterial population density-dependent system known as quorum sensing (QS). To analyze the effect of growth inhibition on QS, the activity of ciprofloxacin, an antibiotic that impairs cell division, was compared to that of phloretin at 50% MIC. Unlike phloretin, the antibiotic hardly affected the tested phenotypes. The use of DH5α, a QS-negative Escherichia coli strain, transformed with an AHL synthase (ExpI) from Pb1692, allowed to validate direct inhibition of AHL production by phloretin, as demonstrated by two biosensor strains, Chromobacterium violaceaum (CV026) and E. coli (pSB401). Expression analysis of virulence-related genes revealed downregulation of QS-regulated genes (expI, expR, luxS, rsmB), plant cell wall degrading enzymes genes (pel, peh and prt) and motility genes (motA, fim, fliA, flhC and flhD) following exposure to both phloretin concentrations. The results support the inhibition of ExpI activity by phloretin. Docking simulations were used to predict the molecular associations between phloretin and the active site of ExpI, to suggest a likely mode of action for the compound’s inhibition of virulence.
When Does Rejection Trigger Aggression? A Test of the Multimotive Model
Authors: Stubbs-Richardson Megan , Sinclair H. Colleen , Porter Ben , Utley Jessica Weiss
Published: June 2021

Research has sought to identify the conditions under which rejection leads to retaliation. The Multimotive Model (MMM) proposes that there are three primary behavioral responses to rejection: prosocial (e.g., befriending others), asocial (e.g., withdrawal), and antisocial behavior (e.g., aggression toward others). In this study, we conducted the first full test of the MMM as well as expanded the model. Based on research linking aggression and “perceived groupness,” construal items were added assessing whether the rejection was perceived as extending beyond the individual to one's peers. We also included self-harm behavioral responses as this outcome was not sufficiently captured by existing antisocial or asocial operationalizations. This expanded model was then tested with two high school student samples (Ns of 231 and 374) who reported experiencing aggressive rejection (i.e., experienced physical, verbal, relational, or cyber aggression from peers). The MMM was compared to a saturated model separately in each of the two datasets using structural equation modeling. Results indicate that the saturated model provides a better fit for the data than the MMM across all models examined (all p < 0.001). In part, this is due to certain paths having different associations than hypothesized. For example, perceiving the rejection as carrying a higher cost was predicted to promote prosocial behavior, where instead it predicted asocial responses. Perceived groupness was the strongest predictor of antisocial responses. Self-harm outcomes were significantly and consistently associated with higher perceived costs across the models. These results and others will be discussed in the context of how we can better encourage prosocial and discourage antisocial and self-harm responses to social rejection, including bullying.
Just Checking It Out? Motivations for and Behavioral Associations With Visiting “Slutpages” in the United States and Australia
Authors: Clancy Elizabeth M. , Maas Megan K. , March Evita , Howard Dominika , Klettke Bianca
Published: June 2021

“Slutpages” are a pernicious form of online image-based evaluative voyeurism (OIBEV), whereby (sexualized) images of women are posted on webpages for (predominantly) male groups to rate and comment. Despite media and public concern, OIBEV sites have garnered limited empirical study. This paper presents the first analysis of OIBEV site visitation motivations across United States and Australian samples. Participants comprised a convenience sample of 1148 young adults aged 18 to 29 years (M = 22.54, SD = 2.50); 53.0% women, 47.0% men; 54% residing in the U.S. and 46% in Australia. Respondents completed an online questionnaire. Overall, 23% of United States and 16% of Australian respondents had visited OIBEV sites. OIBEV site visitation was uniquely associated with gender and country (with men and United States being more likely to visit OIBEV sites), requesting and disseminating sexts and having one’s own image shared. Cyberbullying perpetration was associated with reduced odds of OIBEV site visitation. Motivations differed by gender, with men (80%) being most likely to visit sites to “check them out” while women were equally likely to check it out (41%) or to see if they were depicted (36%). For men, unique predictors of OIBEV site visitation were having requested, disseminated and received disseminated sexts, lower levels of anxiety and reduced likelihood of cyberbullying perpetration. For women, OIBEV site visitation was uniquely associated with being a United States resident, sext dissemination victimization, receipt of disseminated sexts, higher levels of anxiety but reduced stress. Our findings confirm that OIBEV sites represent a highly gendered form of online image-based sexual abuse, and may have important mental health implications, given the associations with increased anxiety. Our results support the need for “slutpage” education for adolescents and young adults to address social and peer norms that encourage and support non-consensual use of intimate images.
Protect ya Grandma! The Effects of Students' Epistemic Beliefs and Prosocial Values on COVID-19 Vaccination Intentions
Authors: Rosman Tom , Adler Kathrin , Barbian Luisa , Blume Vanessa , Burczeck Benno , Cordes Vivien , Derman Dilara , Dertli Susanne , Glas Hannah , Heinen Virginia , Kenst Stefan , Khosroschahli Marie , Kittel Laura , Kraus Corinna , Linden Alica , Mironova Anastasia , Olinger Lena , Rastelica Fatbardh , Sauter Theresia , Schnurr Vera , Schwab Elisabeth , Vieyra Yves , Zidak Andreas , Zidarova Ivana
Published: June 2021

The present study investigates epistemic beliefs (beliefs about the nature of knowledge and knowing) and prosocial values as predictors of COVID-19 vaccination intentions. As a first hypothesis, we posit that beliefs in justification by authority will positively relate to vaccination intentions. Second, we expect a positive relationship between prosocial values and vaccination intentions. Third, we hypothesize that beliefs in justification by authority moderate the relationship between prosocial values and vaccination intentions, so that the positive correlation between prosocial values and vaccination intentions becomes stronger with increasing beliefs in justification by authority. Hypotheses were tested in a sample of N = 314 German university students, a group with rather high mobility, who, when vaccinated, will increase the chance of attaining herd immunity. Hypotheses were tested using correlational and multiple regression analyses. Results revealed a highly significant positive relationship between justification by authority and vaccination intentions, whereas both hypotheses that included prosocial values did not yield significant results. Additional exploratory analyses revealed that the relationship between justification by authority and vaccination intentions was mediated by beliefs in the safety and effectiveness of the vaccines. Furthermore, significant negative relationships were found between personal justification and vaccination intentions as well as between justification by multiple sources and vaccination intentions. These results highlight the crucial role of science and public health communication in fostering vaccination intentions regarding COVID-19.
Students' Perceptions of a Blended Learning Environment to Promote Critical Thinking
Authors: Lu Dan
Published: June 2021

Critical thinking is considered as one of the indispensable skills that must be possessed by the citizens of modern society, and its cultivation with blended learning has drawn much attention from researchers and practitioners. This study proposed the construction of a blended learning environment, where the pedagogical, social, and technical design was directed to fostering critical thinking. The purpose of the study was to find out students' perceptions of the learning environment concerning its design and its influence on their critical thinking. Adopting the mixed method, the study used questionnaire and interview as the instruments for data collection. The analysis of the data revealed that the students generally held positive perceptions of the environment, and they believed that the blended learning environment could help promote their critical thinking in different aspects.
Does Teacher Immediacy Affect Students? A Systematic Review of the Association Between Teacher Verbal and Non-verbal Immediacy and Student Motivation
Authors: Liu Wei
Published: June 2021

In instructional-learning contexts, the relationship between teacher verbal and non-verbal immediacy and student motivation has gained increasing attention. However, no systematic research has been done to review the empirical studies conducted on the impact of teacher immediacy on students' motivation. Hence, the aim of the present study was to systematically review the available literature on different types of teacher immediacy and student motivation. Some common databases were searched and 30 eligible manuscripts were identified. With regard to the key features of the included studies, the review's findings were categorized into different sections, namely “the measures of teacher immediacy employed,” “the measures of student motivation employed,” “designs,” and “educational contexts”. The main findings of the studies were also discussed. The reviewed studies pointed to positive associations between teacher immediacy and student motivation. Finally, limitations of the included studies are discussed and some practical directions for further research are offered, accordingly.
The Geographical Distribution of Human Cutaneous and Visceral Leishmania Species Identified by Molecular Methods in Iran: A Systematic Review With Meta-Analysis
Authors: Hajjaran Homa , Saberi Reza , Borjian Alireza , Fakhar Mahdi , Hosseini Seyed Abdollah , Ghodrati Sajjad , Mohebali Mehdi
Published: June 2021

Leishmaniasis is one of the most common vector-borne parasitic diseases in Iran.Leishmaniaspecies identification is necessary for epidemiological aspects, precise prognosis, control and treatment of the disease. We systematically searched all the studies, reports, and documentation related to species identification and geographical distribution of causative agents of cutaneous (CL), mucosal (ML), and visceral leishmaniasis (VL) using DNA-based molecular diagnostic techniques in Iran. International databases including PubMed, ScienceDirect, Embase, Google Scholar, Scopus, and Web of Science were systemically searched for English articles and Iran's databases including SID, IranMedex and Magiran were searched for Persian reports and articles. Searches were performed from 1999 to 2019 (20 years). The current review was conducted using the keywords: cutaneous leishmaniasis, visceral leishmaniasis,Leishmaniaspecies, Human, Molecular, PCR, and Iran. The study quality was evaluated using the NOS checklist. This meta-analysis procedure was accomplished using STATA, version 2.7.9. Of the 3,426 records identified in the initial search, 154 articles met inclusion criteria and qualified for the systematic review and meta-analysis. In subgroup analysis, the pooled frequency of causative agents of CL isolates was 67.3% (95% CI: 59.51–74.67%) forL. majorand 32.1% (95% CI: 24.72–39.87%) forL. tropica. In addition, the pooled frequency of causative agents of VL isolates was 97.1% (95% CI: 94.6–98.8%) forL. infantumand 2.9% (95% CI: 1.12–5.37%) forL. tropica. The findings of this study showed that the main causative agents of CL and VL in Iran areL. majorandL. infantum, respectively. Moreover, kinetoplast DNA (kDNA) and internal transcriber spacer (ITS) were the most used markers for identifyingLeishmaniaspecies. The current study provides valuable data to encourage and direct researchers as well as public health managers in the comprehensive leishmaniasis control and prevention planning in Iran.
Psychological and Demographic Determinants of Substance Use and Mental Health During the COVID-19 Pandemic
Authors: Mougharbel Fatima , Sampasa-Kanyinga Hugues , Heidinger Brandon , Corace Kim , Hamilton Hayley A. , Goldfield Gary S.
Published: June 2021

Background:Alcohol consumption and distress have increased among Canadians since the start of the COVID-19 pandemic.

Methods:We examined whether sociodemographic and COVID-19-related worries are associated with various combinations of alcohol consumption and comorbid psychological distress variables among a Canadian sample of adults. Data were derived from a sample of Canadian adults (N= 1,005, 49.6% female) who participated in an online survey in May 2020. Four multivariate ordinal logistic regression models were used to estimate the odds of binge drinking, increased alcohol consumption during the pandemic, and psychological distress. Predictor variables used in the analyses included self-reported sociodemographic characteristics, financial worries, COVID-19 impact on work, and worrying about getting ill.

Results:Women were found to have higher odds of increased drinking and anxiety. Also being divorced, separated, or widowed was associated with higher odds of binge drinking and anxiety, and binge drinking and depression. Furthermore, being 60 or older was associated with lower odds of binge drinking and depression and increased drinking and depression, as well as lower odds of increased drinking and depression and increased drinking and anxiety. High income groups were associated with higher odds of binge drinking, increased drinking, and mental distress. Compared to those less worried, being very worried about finances were associated with higher odds of binge drinking and anxiety, increased drinking and anxiety, and increased drinking and depression. Also, being very worried about getting ill with COVID was associated with higher odds of binge drinking and anxiety and increased drinking and anxiety.

Conclusion:Our findings identify several demographic and COVID-related worries for increased odds of alcohol intake and co-morbid psychological distress during the COVID-19 pandemic, including identifying as a woman, high income groups, being divorced, separated or widowed, and experiencing financial worries and COVID illness worries. These characteristics should be considered when developing prevention and treatment programs for adults with problematic alcohol use and comorbid anxiety and depression.
Knowledge, Attitudes, and Practices of Military Personnel Regarding Heat-Related Illness Risk Factors: Results of a Chinese Cross-Sectional Study
Authors: Wang Xuren , Xia Demeng , Long Xisha , Wang Yixin , Wu Kaiwen , Xu Shuogui , Gui Li
Published: June 2021

Background:Military personnel are widely exposed to risk factors for heat-related illnesses. Knowledge, attitudes, and practices (KAP) are three of the most important means by which to prevent such illnesses, but there has been a lack of investigations into and correlation analyses of KAP. This study aimed to explore the heat-related KAP of military personnel in China.

Methods:We conducted a cross-sectional study (June 1-25, 2019). A total of 646 military personnel were recruited from two Chinese Navy troops in the tropical zone and one troop in the temperate zone. We collected data on demographic characteristics and KAP scores using questionnaires. Univariate analysis and Scheffe's method were used for data analyses.

Results:The mean KAP scores were 10.37 (range = 3–13, standard deviation = 1.63) for knowledge (K-score), 7.76 (range = 0–16, SD = 2.65) for attitudes (A-score), and 3.80 (range = 1–6, SD = 1.12) for practices (P-score). There were noticeable differences in mean K-score according to age, military rank, and educational level (P< 0.05). Participants from the tropical zone had higher A-scores (P< 0.05) and higher P-scores (P< 0.001) than those from the temperate zone. Additionally, participants with relevant experience also had higher A-scores (P< 0.05) than those without such experience.

Conclusions:Military personnel's awareness of preventive and first-aid measures against heat-related illnesses need to be strengthened. It will be very important to develop educational programmes and enrich systematic educational resources to raise this awareness.
Using Literature Based Discovery to Gain Insights Into the Metabolomic Processes of Cardiac Arrest
Authors: Henry Sam , Wijesinghe D. Shanaka , Myers Aidan , McInnes Bridget T.
Published: June 2021

In this paper, we describe how we applied LBD techniques to discover lecithin cholesterol acyltransferase (LCAT) as a druggable target for cardiac arrest. We fully describe our process which includes the use of high-throughput metabolomic analysis to identify metabolites significantly related to cardiac arrest, and how we used LBD to gain insights into how these metabolites relate to cardiac arrest. These insights lead to our proposal (for the first time) of LCAT as a druggable target; the effects of which are supported byin vivostudies which were brought forth by this work. Metabolites are the end product of many biochemical pathways within the human body. Observed changes in metabolite levels are indicative of changes in these pathways, and provide valuable insights toward the cause, progression, and treatment of diseases. Following cardiac arrest, we observed changes in metabolite levels pre- and post-resuscitation. We used LBD to help discover diseases implicitly linked via these metabolites of interest. Results of LBD indicated a strong link between Fish Eye disease and cardiac arrest. Since fish eye disease is characterized by an LCAT deficiency, it began an investigation into the effects of LCAT and cardiac arrest survival. In the investigation, we found that decreased LCAT activity may increase cardiac arrest survival rates by increasingω-3 polyunsaturated fatty acid availability in circulation. We verified the effects ofω-3 polyunsaturated fatty acids on increasing survival rate following cardiac arrest viain vivowith rat models.
Brainstorming With a Social Robot Facilitator: Better Than Human Facilitation Due to Reduced Evaluation Apprehension?
Authors: Geerts Julia , de Wit Jan , de Rooij Alwin
Published: June 2021

Brainstorming is a creative technique used to support productivity and creativity during the idea generation phase of an innovation process. In professional practice, a facilitator structures, regulates, and motivates those behaviors of participants that help maintain productivity and creativity during a brainstorm. Emerging technologies, such as social robots, are being developed to support or even automate the facilitator’s role. However, little is known about whether and how brainstorming with a social robot influences productivity. To take a first look, we conducted a between-subjects experiment (N = 54) that explored 1) whether brainstorming with a Wizard-of-Oz operated robot facilitator, compared to with a human facilitator, influences productivity; and 2) whether any effects on productivity might be explained by the robot’s negative effects on social anxiety and evaluation apprehension. The results showed no evidence for an effect of brainstorming with a teleoperated robot facilitator, compared to brainstorming directly with a human facilitator, on productivity. Although the results did suggest that overall, social anxiety caused evaluation apprehension, and evaluation apprehension negatively affected productivity, there was no effect of brainstorming with a robot facilitator on this relationship. Herewith, the present study contributes to an emerging body of work on the efficacy and mechanisms of the facilitation of creative work by social robots.
Oncofertility in Islam: The Malaysian Perspective
Authors: Ahmad Mohd Faizal , Nik Abdul Ghani Nik Abdul Rahim , Abu Muhammad Azrai , Abdul Karim Abdul Kadir , Shafiee Mohamad Nasir
Published: June 2021

The implementation of oncofertility services is of great importance among cancer survivors of reproductive age to ensure a good quality of life. However, the uptake of this service among Muslim patients is very challenging because of inconclusive laws or evidence based on the Islamic perspective. Hence, we summarized the limited evidence available to consolidate current recommendations for oncofertility practices in the Muslim population in Malaysia. The available “fatwa” and “muzakarah” regarding reproductive procedures and gamete cryopreservation in Islam was searched from the recommendations of local and international bodies, including published and unpublished sources. The relevant information was then extracted according to the current understanding of dilemmas in oncofertility practices in Malaysia, tabulated, and consolidated. Most of the available “fatwa” has been revised by recent “muzakarah” to suit current oncofertility practices. Most Islamic organizations support the practice of oncofertility in Muslim hence indicates that oncofertility services are permissible. Therefore, proper recommendations and counseling are paramount to ensure understanding among Muslim patients.
Development of Recombinase Aided Amplification Combined With Disposable Nucleic Acid Test Strip for Rapid Detection of Porcine Circovirus Type 2
Authors: Chen Wenxian , Fan Jindai , Li Zhaoyao , Zhang Yuanyuan , Qin Yuwei , Wu Keke , Li Xiaowen , Li Yuwan , Fan Shuangqi , Zhao Mingqiu
Published: June 2021

Porcine circovirus type 2 (PCV2) is the dominant causative agent of PCV2 systemic disease (PCV2-SD) in pigs. It can also associate with other diseases such as respiratory and enteric diseases, reproductive failure, porcine dermatitis and nephropathy syndrome in pigs. Currently, PCV2 infection is a considerable threat in the swine industry. Therefore, it is of great significance to prevent, control, and accurately detect PCV2 in pig farms. Recombinase aided amplification (RAA) technology is an isothermal nucleic acid amplification technology that could rapidly amplify the target gene fragment at a constant temperature. The amplification products labeled with specific molecules could be visually detected using the test strip with the corresponding antibody. In the present study, the RAA technology combined with a nucleic acid test strip (RAA-strip) was established for simple and specific detection of PCV2. Primers and probes targeting the PCV2 ORF2 gene were designed according to the RAA technology principles. The PCV2 RAA-strip established in this study could detect as low as 103 copies/μL of recombinant plasmids containing the PCV2 ORF2 gene fragment. The lowest detection limit about viral DNA and virus titers was 6.7 × 10−6 ng/μL and 10 TCID50/mL, respectively. Furthermore, no cross-reaction with other porcine viruses occurred at 37°C and within 15 min. We used 42 clinical samples to assess the performance of our established method. The positive rate of clinical samples detected by PCV2 RAA-strip was 50.00%. This was similar to that detected by PCV2 PCR (45.24%). In conclusion, due to the advantages of strong specificity, high sensitivity, excellent reproducibility, and simple operation method, our PCV2 RAA-strip is suitable for the rapid clinical detection of PCV2 on-site.
Convolutional Neural Network with an Elastic Matching Mechanism for Time Series Classification
Authors: Ouyang Kewei , Hou Yi , Zhou Shilin , Zhang Ye
Published: June 2021

Recently, some researchers adopted the convolutional neural network (CNN) for time series classification (TSC) and have achieved better performance than most hand-crafted methods in the University of California, Riverside (UCR) archive. The secret to the success of the CNN is weight sharing, which is robust to the global translation of the time series. However, global translation invariance is not the only case considered for TSC. Temporal distortion is another common phenomenon besides global translation in time series. The scale and phase changes due to temporal distortion bring significant challenges to TSC, which is out of the scope of conventional CNNs. In this paper, a CNN architecture with an elastic matching mechanism, which is named Elastic Matching CNN (short for EM-CNN), is proposed to address this challenge. Compared with the conventional CNN, EM-CNN allows local time shifting between the time series and convolutional kernels, and a matching matrix is exploited to learn the nonlinear alignment between time series and convolutional kernels of the CNN. Several EM-CNN models are proposed in this paper based on diverse CNN models. The results for 85 UCR datasets demonstrate that the elastic matching mechanism effectively improves CNN performance.
A Comparison of Rice Husks and Peanut Shells as Bedding Materials on Dairy Cows’ Preferences, Behaviour, and Health
Authors: Li Pengtao , Cai Amin , Descovich Kris , Fu Tong , Lian Hongxia , Gao Tengyun , Phillips Clive J. C.
Published: June 2021

The provision and quality of bedding materials affect the behaviour, welfare, and health of dairy cows. The objective of this study was to evaluate the preference, behaviour, cleanliness, and physiological status of cows on three bedding materials, peanut shells, rice husks, and a combination of two-thirds peanut shells, one-third rice husk. In an initial experiment, 15 nonlactating, pregnant Holstein cows had free access to all 3 bedding treatments for 39 d. Cows spent more time lying down on rice husk (337 min/d) than on peanut–rice combination (212 min/d) and peanut shell (196 min/d) (p < 0.05), and lay down most often on rice husk (4.35 bouts/d) than on peanut shell (2.55 bouts/d) (p < 0.05) but did not differ between peanut shells and peanut–rice combinations in terms of lying time and lying bouts. In Experiment 2, 12 nonlactating cows were used to assess the effects of the 3 bedding materials on dairy cow behaviour, cleanliness, serum indicators, and productivity. The total duration of lying down (PS: 699.1 min/d, PRC: 645.6 min/d, RH: 852.5 min/d), the frequency of bouts of lying down (PS: 8.7 bouts/d, PRC: 7.6 bouts/d, RH: 11.1 bouts/d), and the mean duration of lying bouts (PS: 83.5 min/bouts, PRC: 91.8 min/bouts, RH: 81.4 min/bouts) did not differ between treatments. Similarly, no differences in eating or drinking behaviour of dairy cows were observed. In terms of hygiene, cleanliness scores did not differ between the three bedding materials, but udder and flank cleanliness decreased and improved, respectively. In addition, treatments did not affect serum metabolites or productivity of the cows. In summary, daily behaviour, serum metabolites, and productivity of dairy cows were all within the normal range, and no statistical differences occurred between the three bedding materials, although cows showed a preference for rice husk when given access to all three bedding materials at the same time. Finally, the results suggest that bedding comprised of peanut shells and peanut–rice combinations are all suitable for maintaining the health and comfort of dairy cows.