bluuebunny/crossref_metadata_2025 huggingface dataset:
How Prediction Accuracy Can Affect the Decision-Making Process in Pavement Management System
Authors: Hosseini Seyed Amirhossein , Smadi Omar
Published: February 2021

One of the most important components of pavement management systems is predicting the deterioration of the network through performance models. The accuracy of the prediction model is important for prioritizing maintenance action. This paper describes how the accuracy of prediction models can have an effect on the decision-making process in terms of the cost of maintenance and rehabilitation activities. The process is simulating the propagation of the error between the actual and predicted values of pavement performance indicators. Different rate of error (10%, 30%, 50%, 70%, and 90%) was added into the result of prediction models. The results showed a strong correlation between the prediction models’ accuracy and the cost of maintenance and rehabilitation activities. The cost of treatment (in millions of dollars) over 20 years for five different scenarios increased from ($54.07–$92.95), ($53.89–$155.48), and ($74.41–$107.77) for asphalt, composite, and concrete pavement types, respectively. Increasing the rate of error also contributed to the prediction model, resulting in a higher benefit reduction rate.
Melissococcus plutonius Can Be Effectively and Economically Detected Using Hive Debris and Conventional PCR
Authors: Biová Jana , Charrière Jean-Daniel , Dostálková Silvie , Škrabišová Mária , Petřivalský Marek , Bzdil Jaroslav , Danihlík Jiří
Published: February 2021

European foulbrood (EFB) is an infectious disease of honey bees caused by the bacterium Melissococcus plutonius. A method for DNA isolation and conventional PCR diagnosis was developed using hive debris, which was non-invasively collected on paper sheets placed on the bottom boards of hives. Field trials utilized 23 honey bee colonies with clinically positive symptoms and 21 colonies without symptoms. Bayes statistics were applied to calculate the comparable parameters for EFB diagnostics when using honey, hive debris, or samples of adult bees. The reliability of the conventional PCR was 100% at 6.7 × 103 Colony Forming Unit of M. plutonius in 1 g of debris. The sensitivity of the method for the sampled honey, hive debris, and adult bees was 0.867, 0.714, and 1.000, respectively. The specificity for the tested matrices was 0.842, 0.800, and 0.833. The predictive values for the positive tests from selected populations with 52% prevalence were 0.813, 0.833, and 0.842, and the real accuracies were 0.853, 0.750, and 0.912, for the honey, hive debris, and adult bees, respectively. It was concluded that hive debris can effectively be utilized to non-invasively monitor EFB in honey bee colonies.
Shifts in Ecological Dominance between Two Lepidopteran Species in Refuge Areas of Bt Cotton
Authors: Malaquias José Bruno , Santana Danilo Renato Santiago , Degrande Paulo Eduardo , Ferreira Claudia Pio , de Melo Elmo Pontes , Godoy Wesley Augusto Conde , Pachú Jéssica Karina da Silva , de Sousa Ramalho Francisco , Omoto Celso , de Azevedo Pereira Alexandre Igor , Guazina Renato Anastacio
Published: February 2021

Competition behavior involving agricultural pest species has long been viewed as a powerful selective force that drives ecological and phenotypic diversity. In this context, a Game Theory-based approach may be useful to describe the decision-making dilemma of a competitor with impacts to guarantee its superiority in terms of ecological dominance or sharing of the food resource with its competitor. In an attempt to elucidate the consequences of competitive dynamics for the ecological dominance of these species in refuge areas of Bt cotton, we conducted a study that was divided into two parts. The first study consisted of an evaluation of interactions involving Spodoptera frugiperda (JE Smith, 1797) and Helicoverpa armigera (Hübner, 1808) on non-Bt cotton plants in a field trial. In the second study, we explored the data matrix collected in the field to parameterize a model of Cellular Automata (CA) with update rules inspired by Game Theory. Computer simulations were analyzed in hypothetical scenarios involving the application (or not) of insecticides in the refuge areas in combination with the resistance factor of one or both pest species to the insecticides used in the refuge areas. H. armigera had superior competitive performance in relation to S. frugiperda only at high densities. According to the density-mediated shift in dominance of the species, the resistance of S. frugiperda to insecticides is seen as a risk factor for the production of susceptible individuals of H. armigera on a large scale in the refuge areas. Additionally, S. frugiperda insecticide resistance may potentially impact the resistance evolution of the H. armigera population to Bt cotton. Thus, ecological dominance could diverge by the presence of a resistance allele to insecticides with interspecific competition perhaps subordinate to evolutionary processes.
Effects of Ashwagandha (Withania somnifera) on Physical Performance: Systematic Review and Bayesian Meta-Analysis
Authors: Bonilla Diego A. , Moreno Yurany , Gho Camila , Petro Jorge L. , Odriozola-Martínez Adrián , Kreider Richard B.
Published: February 2021

Ashwagandha (Withania somnifera) is considered a potent adaptogen and anti-stress agent that could have some potential to improve physical performance. This preferred reporting items for systematic reviews and meta-analyses (PRISMA)-based comprehensive systematic review and Bayesian meta-analysis aimed to evaluate clinical trials up to 2020 from PubMed, ScienceDirect, and Google Scholar databases regarding the effect of Ashwagandha supplementation on physical performance in healthy individuals. Besides implementing estimation statistics analysis, we developed Bayesian hierarchical models for a pre-specified subgroup meta-analysis on strength/power, cardiorespiratory fitness and fatigue/recovery variables. A total of 13 studies met the requirements of this systematic review, although only 12 were included in the quantitative analysis. A low-to-moderate overall risk of bias of the trials included in this study was detected. All Bayesian hierarchical models converged to a target distribution (Ȓ = 1) for both meta-analytic effect size (μ) and between-study standard deviation (τ). The meta-analytic approaches of the included studies revealed that Ashwagandha supplementation was more efficacious than placebo for improving variables related to physical performance in healthy men and female. In fact, the Bayesian models showed that future interventions might be at least in some way beneficial on the analyzed outcomes considering the 95% credible intervals for the meta-analytic effect size. Several practical applications and future directions are discussed, although more comparable studies are needed in exercise training, and athletic populations are needed to derive a more stable estimate of the true underlying effect.
Domain Adaptation for Medical Image Segmentation: A Meta-Learning Method
Authors: Zhang Penghao , Li Jiayue , Wang Yining , Pan Judong
Published: February 2021

Convolutional neural networks (CNNs) have demonstrated great achievement in increasing the accuracy and stability of medical image segmentation. However, existing CNNs are limited by the problem of dependency on the availability of training data owing to high manual annotation costs and privacy issues. To counter this limitation, domain adaptation (DA) and few-shot learning have been extensively studied. Inspired by these two categories of approaches, we propose an optimization-based meta-learning method for segmentation tasks. Even though existing meta-learning methods use prior knowledge to choose parameters that generalize well from few examples, these methods limit the diversity of the task distribution that they can learn from in medical image segmentation. In this paper, we propose a meta-learning algorithm to augment the existing algorithms with the capability to learn from diverse segmentation tasks across the entire task distribution. Specifically, our algorithm aims to learn from the diversity of image features which characterize a specific tissue type while showing diverse signal intensities. To demonstrate the effectiveness of the proposed algorithm, we conducted experiments using a diverse set of segmentation tasks from the Medical Segmentation Decathlon and two meta-learning benchmarks: model-agnostic meta-learning (MAML) and Reptile. U-Net and Dice similarity coefficient (DSC) were selected as the baseline model and the main performance metric, respectively. The experimental results show that our algorithm maximally surpasses MAML and Reptile by 2% and 2.4% respectively, in terms of the DSC. By showing a consistent improvement in subjective measures, we can also infer that our algorithm can produce a better generalization of a target task that has few examples.
Clinical Utility of Artificial Intelligence Algorithms to Enhance Wide-Field Optical Coherence Tomography Angiography Images
Authors: Mc Grath Orlaith , Sarfraz Mohammad W. , Gupta Abha , Yang Yan , Aslam Tariq
Published: February 2021

The aim of this paper is to investigate the clinical utility of the application of deep learning denoise algorithms on standard wide-field Optical Coherence Tomography Angiography (OCT-A) images. This was a retrospective case-series assessing forty-nine 10 × 10 mm OCT-A1 macula scans of 49 consecutive patients attending a medical retina clinic over a 6-month period. Thirty-seven patients had pathology; 13 had none. Retinal vascular layers were categorised into superficial or deep capillary plexus. For each category, the retinal experts compared the original standard image with the same image that had intelligent denoise applied. When analysing the Superficial Capillary Plexus (SCP), the denoised image was selected as “best for clinical assessment” in 98% of comparisons. No difference was established in the remaining 2%. On evaluating the Deep Capillary Plexus (DCP), the denoised image was preferred in 35% of comparisons. No difference was found in 65%. There was no evidence of new artefactual features nor loss of anatomical detail in denoised compared to the standard images. The wide-field denoise feature of the Canon Xephilio OCT-A1 produced scans that were clinically preferable over their original OCT-A images, especially for SCP assessment, without evidence for causing a new artefactual error.
Fundamental Investigation of Diamond Cutting of Micro V-Shaped Grooves on a Polycrystalline Soft-Brittle Material
Authors: Huang Weihai , Yan Jiwang
Published: February 2021

Fabricating micro-structures on optical materials has received great interest in recent years. In this work, micro-grooving experiments were performed on polycrystalline zinc selenide (ZnSe) to investigate the feasibility of surface micro-structuring on polycrystalline soft-brittle material by diamond turning. A photosensitive resin was coated on the workpiece before cutting, and it was found that the coating was effective in suppressing brittle fractures at the edges of the grooves. The effect of tool feed rate in groove depth direction was examined. Results showed that the defect morphology on the groove surface was affected by the tool feed rate. The crystallographic orientation of grains around the groove was characterized by electron backscatter diffraction (EBSD), and it was found that the formation of defects was strongly dependent on the angle of groove surface with respect to the cleavage plane of grain. The stress distribution of the micro-grooving process was investigated by the finite element method. Results showed that the location of tensile stresses in the coated workpiece was farther from the edge of the groove compared with that in the uncoated workpiece, verifying the experimental result that brittle fractures were suppressed by the resin coating.
Numerical and Experimental Investigation of the Impact of the Electromagnetic Properties of the Die Materials in Electromagnetic Forming of Thin Sheet Metal
Authors: Beckschwarte Björn , Langstädtler Lasse , Schenck Christian , Herrmann Marius , Kuhfuss Bernd
Published: February 2021

In electromagnetic forming of thin sheet metal, the die is located within the effective range of the electromagnetic wave. Correspondingly, a current is induced not only in the sheet metal, but also in the die. Like the current in the workpiece, also the current in the die interacts with the electromagnetic wave, resulting in Lorentz forces and changes of the electromagnetic field. With the aim to study the influence of different electromagnetic die properties in terms of specific electric resistance and relative magnetic permeability, electromagnetic simulations were carried out. A change in the resulting forming forces in the sheet metals was determined. To confirm the simulation results, electromagnetic forming and embossing tests were carried out with the corresponding die materials. The results from simulation and experiment were in good agreement.
Migrants in the Attic: The Case of Migrants with Disabilities and Resettlement Services in Serbia
Authors: Badali Joel John
Published: February 2021

The global migrant crisis triggered an unprecedented number of asylum seekers in the Balkan region. In this case study, the state of migrants with disabilities—a community notoriously overlooked during global conflict—is explored through field interviews of settlement service providers in Serbia. A human rights framework is espoused in first examining contemporary refugee law discourse and the corresponding gaps in current resettlement practice of migrants with disabilities. The study’s findings illuminate the need for a drastic shift in settlement services for those migrants most vulnerable to persecution in de facto destination countries. The discussion takes aim at “humanitarian silo” funding models and argues for international cooperation and transparency in accommodating migrants with disabilities internationally.
A Novel Application on the Drive Elements of Using Electrical Contact Resistance and Friction Coefficient for Evaluating Induction Heat Treatment
Authors: Chang Yuh-Ping , Wang Hsiang-Yu , Chou Huann-Ming
Published: February 2021

The load on drive elements under extreme pressure conditions is significantly larger than that used in machine tools. When operating under a heavy load for a long period, large deformation and severe wear between the ball and the track are more likely to occur. To reduce wear, the most fundamental solution is to improve the surface properties of the material. Moreover, heat treatment is the most effective method to improve the surface properties of materials, thereby achieving wear resistance and low friction. It is necessary to develop a new heat treatment technology for wear resistance in extreme pressure conditions. Therefore, this study conducted experiments using a reciprocating friction tester. The responses of electrical contact resistance and the friction coefficient were measured synchronously to investigate wear resistance and low friction of the alloy steels after the induction heat treatment. Then, the results were compared and verified with low-carbon alloy steel after the traditional carburizing heat treatment. The experimental results show that the application of new induction heat treatment technology can not only improve the performance of drive components, but also save time and energy, and streamline the production process of the drive components. Therefore, the results of these wear analyses confirm that the induction heat treatment mode can replace the traditional carburizing heat treatment mode for drive elements.
Properties and Structure of Concretes Doped with Production Waste of Thermoplastic Elastomers from the Production of Car Floor Mats
Authors: Ulewicz Malgorzata , Pietrzak Alina
Published: February 2021

This article presents physical and mechanical properties of concrete composites that include waste thermoplastic elastomer (TPE) from the production process of car floor mats. Waste elastomer (2–8 mm fraction) was used as a substitute for fine aggregate in quantities of 2.5, 5.0, 7.5, and 10% of the cement weight. For all series of concrete, the following tests were carried out: compression strength, bending tensile strength, splitting tensile strength, absorbability, density, resistance to water penetration under pressure, frost resistance, and abrasion resistance, according to applicable standards. Moreover, SEM/EDS analysis was carried out on the surface microstructure of synthesized concrete composites. It was proven that the use of production waste from the production process of car floor mats in the quantity of 2.5% does not influence the change of the concrete microstructure and it does not result in the decrease of the mechanical parameters of concrete modified with waste. All concrete modified with the addition of waste meet standards requirements after carrying out 15 cycles of freezing and thawing, and the average decrease in compression strength did not exceed 20%. Adding waste in the quantity of 2.5% allows for limiting the use of aggregate by about 5%, which is beneficial for the natural environment.
Haloferax mediterranei Cells as C50 Carotenoid Factories
Authors: Giani Micaela , Montero-Lobato Zaida , Garbayo Inés , Vílchez Carlos , Vega José M. , Martínez-Espinosa Rosa María
Published: February 2021

Haloarchaea produce C50 carotenoids such as bacterioruberin, which are of biotechnological in-terest. This study aimed to analyze the effect of different environmental and nutritional conditions on the cellular growth and dynamics of carotenoids accumulation in Haloferax mediterranei. The maximum production of carotenoids (40 µg·mL−1) was obtained during the stationary phase of growth, probably due to nutrient-limiting conditions (one-step culture). By seven days of culture, 1 mL culture produced 22.4 mg of dry weight biomass containing 0.18 % (w/w) of carotenoids. On the other hand, carbon-deficient cultures (low C/N ratio) were observed to be optimum for C50 bacterioruberin production by Hfx. mediterranei, but negatively affected the growth of cells. Thus, a two-steps process was evaluated for optimum carotenoids yield. In the first step, a nutri-ent-repleted culture medium enabled the haloarchaea to produce biomass, while in the second step, the biomass was incubated under osmotic stress and in a carbon-deficient medium. Under the conditions used, the obtained biomass contained 0.27% (w/w) of carotenoids after seven days, which accounts for 58.49 µg·mL−1 of carotenoids for a culture with turbidity 14.0.
A New, Quick, and Simple Protocol to Evaluate Microalgae Polysaccharide Composition
Authors: Decamp Antoine , Michelo Orane , Rabbat Christelle , Laroche Céline , Grizeau Dominique , Pruvost Jérémy , Gonçalves Olivier
Published: February 2021

In this work, a new methodological approach, relying on the high specificity of enzymes in a complex mixture, was developed to estimate the composition of bioactive polysaccharides produced by microalgae, directly in algal cultures. The objective was to set up a protocol to target oligomers commonly known to be associated with exopolysaccharides’ (EPS) nutraceutical and pharmaceutical activities (i.e., rhamnose, fucose, acidic sugars, etc.) without the constraints classically associated with chromatographic methods, while maintaining a resolution sufficiently high to enable their monitoring in the culture system. Determination of the monosaccharide content required the application of acid hydrolysis (2 M trifluoroacetic acid) followed by NaOH (2 M) neutralization. Quantification was then carried out directly on the fresh hydrolysate using enzyme kits corresponding to the main monosaccharides in a pre-determined composition of the polysaccharides under analysis. Initial results showed that the enzymes were not sensitive to the presence of TFA and NaOH, so the methodology could be carried out on fresh hydrolysate. The limits of quantification of the method were estimated as being in the order of the log of nanograms of monosaccharides per well, thus positioning it among the chromatographic methods in terms of analytical performance. A comparative analysis of the results obtained by the enzymatic method with a reference method (high-performance anion-exchange chromatography) confirmed good recovery rates, thus validating the closeness of the protocol. Finally, analyses of raw culture media were carried out and compared to the results obtained in miliQ water; no differences were observed. The new approach is a quick, functional analysis method allowing routine monitoring of the quality of bioactive polysaccharides in algal cultures grown in photobioreactors.
A Comparative Study on the Flocculation of Silica and China Clay with Chitosan and Synthetic Polyelectrolytes
Authors: Borchert Konstantin B. L. , Steinbach Christine , Schwarz Simona , Schwarz Dana
Published: February 2021

Flocculation is still one of the most important and efficient processes for water treatment. However, most industrial processes, such as in water treatment plants, still use huge amounts of synthetic polyelectrolytes for the flocculation process. Here we compare the flocculation of two different suspended particles, i.e., silica particles and china clay, with the biopolymer chitosan and two common strong synthetic polyelectrolytes. As a flocculant, chitosan featured a minimum uptake rate of 0.05 mg/g for silica and 1.8 mg/g for china clay. Polydiallyldimethylammonium chloride (PDADMAC) for comparison possessed a minimum uptake rate of 0.05 mg/g for silica and 2.2 mg/g for china clay. Chitosan as an environmentally friendly biopolymer competes with the synthetic polyelectrolytes and thus represents a beneficial economic alternative to synthetic flocculants.
Subcutaneous ω-Conotoxins Alleviate Mechanical Pain in Rodent Models of Acute Peripheral Neuropathy
Authors: Hasan Md. Mahadhi , Starobova Hana , Mueller Alexander , Vetter Irina , Lewis Richard J.
Published: February 2021

The peripheral effects of ω-conotoxins, selective blockers of N-type voltage-gated calcium channels (CaV2.2), have not been characterised across different clinically relevant pain models. This study examines the effects of locally administered ω-conotoxin MVIIA, GVIA, and CVIF on mechanical and thermal paw withdrawal threshold (PWT) in postsurgical pain (PSP), cisplatin-induced neuropathy (CisIPN), and oxaliplatin-induced neuropathy (OIPN) rodent models. Intraplantar injection of 300, 100 and 30 nM MVIIA significantly (p < 0.0001, p < 0.0001, and p < 0.05, respectively) alleviated mechanical allodynia of mice in PSP model compared to vehicle control group. Similarly, intraplantar injection of 300, 100, and 30 nM MVIIA (p < 0.0001, p < 0.01, and p < 0.05, respectively), and 300 nM and 100 nM GVIA (p < 0.0001 and p < 0.05, respectively) significantly increased mechanical thresholds of mice in OIPN model. The ED50 of GVIA and MVIIA in OIPN was found to be 1.8 pmol/paw and 0.8 pmol/paw, respectively. However, none of the ω-conotoxins were effective in a mouse model of CisIPN. The intraplantar administration of 300 nM GVIA, MVIIA, and CVIF did not cause any locomotor side effects. The intraplantar administration of MVIIA can alleviate incision-induced mechanical allodynia, and GVIA and MVIIA effectively reduce OIPN associated mechanical pain, without locomotor side effects, in rodent models. In contrast, CVIF was inactive in these pain models, suggesting it is unable to block a subset of N-type voltage-gated calcium channels associated with nociceptors in the skin.
Influence of Welding Parameters and Filler Material on the Mechanical Properties of HSLA Steel S960MC Welded Joints
Authors: Mičian Miloš , Frátrik Martin , Kajánek Daniel
Published: February 2021

This article provides an overview of the influence of welding parameters and filler material on changes in the heat-affected zone (HAZ) of thermo-mechanically controlled processed (TMCP) steel welded joints. The research focused on evaluating the effect of heat input and cooling rate on the width of the soft zone, which significantly affects the mechanical properties of welded joints. The negative effect of the soft zone is more pronounced as the thickness of the material decreases. Therefore, the object of this research was a 3-mm-thick sheet of S960MC steel welded by gas metal arc welding (GMAW) and metal-cored arc welding (MCAW) technology. Variable welding parameters were reflected in different heat input and cooling rate values, which led to a change in the properties of the HAZ and thus the mechanical properties of the welded joints. The changes in the HAZ were analyzed by microscopic analysis and mechanical testing. The measured results showed a significant effect of heat input on the cooling rate, which considerably affected the width of the soft zone in the HAZ and thus the overall mechanical properties of the welded joints.
Characterization of Austenitic Stainless Steels with Regard to Environmentally Assisted Fatigue in Simulated Light Water Reactor Conditions
Authors: Bruchhausen Matthias , Dundulis Gintautas , McLennan Alec , Arrieta Sergio , Austin Tim , Cicero Román , Chitty Walter-John , Doremus Luc , Ernestova Miroslava , Grybenas Albertas , Huotilainen Caitlin , Mann Jonathan , Mottershead Kevin , Novotny Radek , Perosanz Francisco Javier , Platts Norman , le Roux Jean-Christophe , Spätig Philippe , Torre Celeizábal Claudia , Twite Marius , Vankeerberghen Marc
Published: February 2021

A substantial amount of research effort has been applied to the field of environmentally assisted fatigue (EAF) due to the requirement to account for the EAF behaviour of metals for existing and new build nuclear power plants. We present the results of the European project INcreasing Safety in NPPs by Covering Gaps in Environmental Fatigue Assessment (INCEFA-PLUS), during which the sensitivities of strain range, environment, surface roughness, mean strain and hold times, as well as their interactions on the fatigue life of austenitic steels has been characterized. The project included a test campaign, during which more than 250 fatigue tests were performed. The tests did not reveal a significant effect of mean strain or hold time on fatigue life. An empirical model describing the fatigue life as a function of strain rate, environment and surface roughness is developed. There is evidence for statistically significant interaction effects between surface roughness and the environment, as well as between surface roughness and strain range. However, their impact on fatigue life is so small that they are not practically relevant and can in most cases be neglected. Reducing the environmental impact on fatigue life by modifying the temperature or strain rate leads to an increase of the fatigue life in agreement with predictions based on NUREG/CR-6909. A limited sub-programme on the sensitivity of hold times at elevated temperature at zero force conditions and at elevated temperature did not show the beneficial effect on fatigue life found in another study.
External-Field-Induced Phase Transformation and Associated Properties in a Ni50Mn34Fe3In13 Metamagnetic Shape Memory Wire
Authors: Chen Zhen , Cong Daoyong , Li Shilei , Zhang Yin , Li Shaohui , Cao Yuxian , Li Shengwei , Song Chao , Ren Yang , Wang Yandong
Published: February 2021

Metamagnetic shape memory alloys exhibit a series of intriguing multifunctional properties and have great potential for applications in magnetic actuation, sensing and magnetic refrigeration. However, the poor mechanical properties of these alloys with hardly any tensile deformability seriously limit their practical application. In the present work, we developed a Ni-Fe-Mn-In microwire that exhibits both a giant, tensile superelasticity and a magnetic-field-induced first-order phase transformation. The recoverable strain of superelasticity is more than 20% in the temperature range of 233–283 K, which is the highest recoverable strain reported heretofore in Ni-Mn-based shape memory alloys (SMAs). Moreover, the present microwire exhibits a large shape memory effect with a recoverable strain of up to 13.9% under the constant tensile stress of 225 MPa. As a result of the magnetic-field-induced first-order phase transformation, a large reversible magnetocaloric effect with an isothermal entropy change ΔSm of 15.1 J kg−1 K−1 for a field change from 0.2 T to 5 T was achieved in this microwire. The realization of both magnetic-field and tensile-stress-induced transformations confers on this microwire great potential for application in miniature multi-functional devices and provides an opportunity for multi-functional property optimization under coupled multiple fields.
Influence of Successive Chemical and Thermochemical Treatments on Surface Features of Ti6Al4V Samples Manufactured by SLM
Authors: González Jesús E. , Armas Gabriela de , Negrin Jeidy , Beltrán Ana M. , Trueba Paloma , Gotor Francisco J. , Peón Eduardo , Torres Yadir
Published: February 2021

Ti6Al4V samples, obtained by selective laser melting (SLM), were subjected to successive treatments: acid etching, chemical oxidation in hydrogen peroxide solution and thermochemical processing. The effect of temperature and time of acid etching on the surface roughness, morphology, topography and chemical and phase composition after the thermochemical treatment was studied. The surfaces were characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and contact profilometry. The temperature used in the acid etching had a greater influence on the surface features of the samples than the time. Acid etching provided the original SLM surface with a new topography prior to oxidation and thermochemical treatments. A nanostructure was observed on the surfaces after the full process, both on their protrusions and pores previously formed during the acid etching. After the thermochemical treatment, the samples etched at 40 °C showed macrostructures with additional submicro and nanoscale topographies. When a temperature of 80 °C was used, the presence of micropores and a thicker anatase layer, detectable by X-ray diffraction, were also observed. These surfaces are expected to generate greater levels of bioactivity and high biomechanics fixation of implants as well as better resistance to fatigue.
Metabolic Alterations Associated with γ-Hydroxybutyric Acid and the Potential of Metabolites as Biomarkers of Its Exposure
Authors: Jung Suryun , Kim Suji , Seo Yujin , Lee Sooyeun
Published: February 2021

γ-Hydroxybutyric acid (GHB) is an endogenous short chain fatty acid that acts as a neurotransmitter and neuromodulator in the mammalian brain. It has often been illegally abused or misused due to its strong anesthetic effect, particularly in drug-facilitated crimes worldwide. However, proving its ingestion is not straightforward because of the difficulty in distinguishing between endogenous and exogenous GHB, as well as its rapid metabolism. Metabolomics and metabolism studies have recently been used to identify potential biomarkers of GHB exposure. This mini-review provides an overview of GHB-associated metabolic alterations and explores the potential of metabolites for application as biomarkers of GHB exposure. For this, we discuss the biosynthesis and metabolism of GHB, analytical issues of GHB in biological samples, alterations in metabolic pathways, and changes in the levels of GHB conjugates in biological samples from animal and human studies. Metabolic alterations in organic acids, amino acids, and polyamines in urine enable discrimination between GHB-ingested animals or humans and controls. The potential of GHB conjugates has been investigated in a variety of clinical settings. Despite the recent growth in the application of metabolomics and metabolism studies associated with GHB exposure, it remains challenging to distinguish between endogenous and exogenous GHB. This review highlights the significance of further metabolomics and metabolism studies for the discovery of practical peripheral biomarkers of GHB exposure.
Ranking Metabolite Sets by Their Activity Levels
Authors: McLuskey Karen , Wandy Joe , Vincent Isabel , van der Hooft Justin J. J. , Rogers Simon , Burgess Karl , Daly Rónán
Published: February 2021

Related metabolites can be grouped into sets in many ways, e.g., by their participation in series of chemical reactions (forming metabolic pathways), or based on fragmentation spectral similarities or shared chemical substructures. Understanding how such metabolite sets change in relation to experimental factors can be incredibly useful in the interpretation and understanding of complex metabolomics data sets. However, many of the available tools that are used to perform this analysis are not entirely suitable for the analysis of untargeted metabolomics measurements. Here, we present PALS (Pathway Activity Level Scoring), a Python library, command line tool, and Web application that performs the ranking of significantly changing metabolite sets over different experimental conditions. The main algorithm in PALS is based on the pathway level analysis of gene expression (PLAGE) factorisation method and is denoted as mPLAGE (PLAGE for metabolomics). As an example of an application, PALS is used to analyse metabolites grouped as metabolic pathways and by shared tandem mass spectrometry fragmentation patterns. A comparison of mPLAGE with two other commonly used methods (overrepresentation analysis (ORA) and gene set enrichment analysis (GSEA)) is also given and reveals that mPLAGE is more robust to missing features and noisy data than the alternatives. As further examples, PALS is also applied to human African trypanosomiasis, Rhamnaceae, and American Gut Project data. In addition, normalisation can have a significant impact on pathway analysis results, and PALS offers a framework to further investigate this. PALS is freely available from our project Web site.
Simultaneous Quantitation of Lipid Biomarkers for Inflammatory Bowel Disease Using LC–MS/MS
Authors: Chhonker Yashpal S. , Kanvinde Shrey , Ahmad Rizwan , Singh Amar B. , Oupický David , Murry Daryl J.
Published: February 2021

Eicosanoids are key mediators and regulators of inflammation and oxidative stress that are often used as biomarkers for severity and therapeutic responses in various diseases. We here report a highly sensitive LC-MS/MS method for the simultaneous quantification of at least 66 key eicosanoids in a widely used murine model of colitis. Chromatographic separation was achieved with Shim-Pack XR-ODSIII, 150 × 2.00 mm, 2.2 µm. The mobile phase was operated in gradient conditions and consisted of acetonitrile and 0.1% acetic acid in water with a total flow of 0.37 mL/min. This method is sensitive, with a limit of quantification ranging from 0.01 to 1 ng/mL for the various analytes, has a large dynamic range (200 ng/mL), and a total run time of 25 min. The inter- and intraday accuracy (85–115%), precision (≥85%), and recovery (40–90%) met the acceptance criteria per the US Food and Drug Administration guidelines. This method was successfully applied to evaluate eicosanoid metabolites in mice subjected to colitis versus untreated, healthy control mice. In summary, we developed a highly sensitive and fast LC−MS/MS method that can be used to identify biomarkers for inflammation and potentially help in prognosis of the disease in inflammatory bowel disease (IBD) patients, including the response to therapy.
Experimental Study on the Coating Removing Characteristics of High-Pressure Water Jet by Micro Jet Flow
Authors: Ning Dayong , Wang Qibo , Tian Jinxin , Gong Yongjun , Du Hongwei , Chen Shengtao , Hou Jiaoyi
Published: February 2021

In this paper, coating removal characteristics of water jet by micro jet flow affected by cleaning parameters is analyzed. Numerical simulation of fluid field calculates the velocity and pressure distribution of a water jet impinging on a rigid wall, which is used for design experiments of coating removal affected by jet pressure, traversal speed, and repeated impacting times. The removal width is used as a measure of water jet coating removal capability. Experiment results show that the coating removal width is constant, independent with traversal speed or repeated times when total exposure time of waterjet impingement is fixed. According to results of coating removal by a linear moving water jet, this study also analyzes characteristics of coating removal by rotating jet disc, especially residual coating affected by rotational and moving speed of the cleaning disc. The research is helpful to improve the coating removal efficiency of cleaning disc devices.
A Novel Frequency Stabilization Approach for Mass Detection in Nonlinear Mechanically Coupled Resonant Sensors
Authors: Li Lei , Liu Hanbiao , Shao Mingyu , Ma Chicheng
Published: February 2021

Frequency stabilization can overcome the dependence of resonance frequency on amplitude in nonlinear microelectromechanical systems, which is potentially useful in nonlinear mass sensor. In this paper, the physical conditions for frequency stabilization are presented theoretically, and the influence of system parameters on frequency stabilization is analyzed. Firstly, a nonlinear mechanically coupled resonant structure is designed with a nonlinear force composed of a pair of bias voltages and an alternating current (AC) harmonic load. We study coupled-mode vibration and derive the expression of resonance frequency in the nonlinear regime by utilizing perturbation and bifurcation analysis. It is found that improving the quality factor of the system is crucial to realize the frequency stabilization. Typically, stochastic dynamic equation is introduced to prove that the coupled resonant structure can overcome the influence of voltage fluctuation on resonance frequency and improve the robustness of the sensor. In addition, a novel parameter identification method is proposed by using frequency stabilization and bifurcation jumping, which effectively avoids resonance frequency shifts caused by driving voltage. Finally, numerical studies are introduced to verify the mass detection method. The results in this paper can be used to guide the design of a nonlinear sensor.
Effects of Thermal Annealing on Femtosecond Laser Micromachined Glass Surfaces
Authors: Sala Federico , Paié Petra , Martínez Vázquez Rebeca , Osellame Roberto , Bragheri Francesca
Published: February 2021

Femtosecond laser micromachining (FLM) of fused silica allows for the realization of three-dimensional embedded optical elements and microchannels with micrometric feature size. The performances of these components are strongly affected by the machined surface quality and residual roughness. The polishing of 3D buried structures in glass was demonstrated using different thermal annealing processes, but precise control of the residual roughness obtained with this technique is still missing. In this work, we investigate how the FLM irradiation parameters affect surface roughness and we characterize the improvement of surface quality after thermal annealing. As a result, we achieved a strong roughness reduction, from an average value of 49 nm down to 19 nm. As a proof of concept, we studied the imaging performances of embedded mirrors before and after thermal polishing, showing the capacity to preserve a minimum feature size of the reflected image lower than μ5μm. These results allow for us to push forward the capabilities of this enabling fabrication technology, and they can be used as a starting point to improve the performances of more complex optical elements, such as hollow waveguides or micro-lenses.
Size-Exclusion Particle Separation Driven by Micro-Flows in a Quasi-Spherical Droplet: Modelling and Experimental Results
Authors: Marinaro Giovanni , Riekel Christian , Gentile Francesco
Published: February 2021

Aqueous solution droplets are supported quasi contact-free by superhydrophobic surfaces. The convective flow in evaporating droplets allows the manipulation and control of biological molecules in solution. In previous works, super-hydrophobic drops on nano-patterned substrates have been used to analyze otherwise undetectable species in extremely low concentration ranges. Here, we used particle image velocimetry (PIV) for studying the flow field in water droplets containing polystyrene particles on a pillared silicon super-hydrophobic chip. The particles describe vortex-like motions around the droplet center as long as the evaporating droplet maintains a spherical shape. Simulations by a Finite Element Method (FEM) suggest that the recirculating flow is due to the temperature gradient along the droplet rim, generating a shear stress. Notably, the characteristics of the internal flow can be modulated by varying the intensity of the temperature gradient along the drop. We then used the flow-field determined by experiments and an approximate form of the Langevin equation to examine how particles are transported in the drop as a function of particle size. We found that larger particles with an average size of μ36 μm are preferentially transported toward the center of the substrate, differently from smaller particles with a 10-fold lower size that are distributed more uniformly in the drop. Results suggest that solutions of spherical particles on a super-hydrophobic chip can be used to separate soft matter and biological molecules based on their size, similarly to the working principle of a time-of-flight (ToF) mass analyzer, except that the separation takes place in a micro-sphere, with less space, less time, and less solution required for the separation compared to conventional ToF systems.
Editorial for the Special Issue “Development of W-Sn and Rare-Metal Metallogenic Systems in an Orogenic Belt”
Authors: Cuney Michel
Published: February 2021

n/a
Grinding Media Motion and Collisions in Different Zones of Stirred Media Mills
Authors: Fragnière Greta , Naumann Aleksandra , Schrader Marcel , Kwade Arno , Schilde Carsten
Published: February 2021

Product fineness during grinding in stirred media mills is mainly influenced by the specific energy input, the stress energy transferred by the colliding grinding media and the stress frequency. The stress energy from grinding media collisions is heterogeneously distributed in stirred media mills. Herein, in order to characterize the stress energy distribution and the local grinding media collision frequencies, the grinding media motion was calculated using discrete element method (DEM) simulations coupled with computational fluid dynamics (CFD). The local grinding media concentration, velocity profiles, grinding media collisions and stress energies were compared for varied total grinding media fillings and stirrer speeds. It was confirmed that the normalized grinding media velocity profile can be used to divide the grinding chamber into four types of zones that allow the modeling of the stress energy distribution. However, the collision frequency showed very different distributions for varied stirrer velocities and grinding media fillings.
Gold in the Oxidized Ores of the Olympiada Deposit (Eastern Siberia, Russia)
Authors: Silyanov Sergey A. , Sazonov Anatoly M. , Zvyagina Yelena A. , Savichev Andrey A. , Lobastov Boris M.
Published: February 2021

Native gold and its satellite minerals were studied throughout the 300 m section of oxidized ores of the Olympiada deposit (Eastern Siberia, Russia). Three zones are identified in the studied section: Upper Zone ~60 g/t Au; Middle Zone ~3 g/t Au; Lower Zone ~20 g/t Au. Supergene and hypogene native gold have been found in these zones. Supergene gold crystals (~1 μm), their aggregates and their globules (100 nm to 1 μm) predominate in the Upper and less in Middle Zone. Relic hypogene gold particles (flattened, fracture and irregular morphology) are sporadically distributed throughout the section. Spongiform gold occurs in the Lower Zone at the boundary with the bedrock, as well as in the bedrock. This gold formed in the process of oxidation of aurostibite, leaching of impurities and its further dissolution. Hypogene gold is commonly isolated but for supergene gold typically associated with ferric (hydr)oxides. New formation of gold occurred due to oxidation of sulfide ores and release of “invisible” gold, as well as dissolution, mobilization and re-deposition of metallic hypogene gold. A model for the formation of oxidized ores with the participation of meteoric and low-temperature hydrothermal waters has been proposed.
Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior
Authors: Homma-Takeda Shino , Fujishiro Hitomi , Tanaka Izumi , Yakumaru Haruko , Ayama Kyoko , Uehara Akihiro , Oikawa Masakazu , Himeno Seiichiro , Ishihara Hiroshi
Published: February 2021

Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeam-based elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior.
Studying the Stability of the K/Ar Isotopic System of Phlogopites in Conditions of High T, P: 40Ar/39Ar Dating, Laboratory Experiment, Numerical Simulation
Authors: Yudin Denis , Murzintsev Nikolay , Travin Alexey , Alifirova Taisiya , Zhimulev Egor , Novikova Sofya
Published: February 2021

Typically, 40Ar/39Ar dating of phlogopites from deep-seated xenoliths of kimberlite pipes produces estimates that suggest much older ages than those when these pipes were intruded. High-pressure (3 GPa) laboratory experiments enabled the authors to explore the behaviour of argon in the phlogopite structure under the conditions that correspond to the mantle, at the temperatures (from 700 to 1000 °С), far exceeding closure temperature of the K/Ar isotopic system. “Volume diffusion” remains foremost for describing the mobility of argon in phlogopite at high pressures. The mantle material age can be estimated through the dating of the phlogopites from deep-seated xenoliths of kimberlites, employing the 40Ar/39Ar method, subject to correction for a partial loss of radiogenic 40Ar when xenolith moves upwards to the Earth’s surface. The obtained data served as the basis for proposing the behaviour model of the K/Ar isotopic system of minerals in conditions of great depths (lower crust, mantle), and when transporting xenoliths in the kimberlite melt.