bluuebunny/crossref_metadata_2025 huggingface dataset:
Intertwined density waves in a metallic nickelate
Authors: Zhang Junjie , Phelan D. , Botana A. S. , Chen Yu-Sheng , Zheng Hong , Krogstad M. , Wang Suyin Grass , Qiu Yiming , Rodriguez-Rivera J. A. , Osborn R. , Rosenkranz S. , Norman M. R. , Mitchell J. F.
Published: November 2020

Nickelates are a rich class of materials, ranging from insulating magnets to superconductors. But for stoichiometric materials, insulating behavior is the norm, as for most late transition metal oxides. Notable exceptions are the 3D perovskite LaNiO3, an unconventional paramagnetic metal, and the layered Ruddlesden-Popper phases R4Ni3O10, (R = La, Pr, Nd). The latter are particularly intriguing because they exhibit an unusual metal-to-metal transition. Here, we demonstrate that this transition results from an incommensurate density wave with both charge and magnetic character that lies closer in its behavior to the metallic density wave seen in chromium metal than the insulating stripes typically found in single-layer nickelates like La2-xSrxNiO4. We identify these intertwined density waves as being Fermi surface-driven, revealing a novel ordering mechanism in this nickelate that reflects a coupling among charge, spin, and lattice degrees of freedom that differs not only from the single-layer materials, but from the 3D perovskites as well.
Unraveling the influence of non-fullerene acceptor molecular packing on photovoltaic performance of organic solar cells
Authors: Ye Linglong , Weng Kangkang , Xu Jinqiu , Du Xiaoyan , Chandrabose Sreelakshmi , Chen Kai , Zhou Jiadong , Han Guangchao , Tan Songting , Xie Zengqi , Yi Yuanping , Li Ning , Liu Feng , Hodgkiss Justin M. , Brabec Christoph J. , Sun Yanming
Published: November 2020

In non-fullerene organic solar cells, the long-range structure ordering induced by end-group π–π stacking of fused-ring non-fullerene acceptors is considered as the critical factor in realizing efficient charge transport and high power conversion efficiency. Here, we demonstrate that side-chain engineering of non-fullerene acceptors could drive the fused-ring backbone assembly from a π–π stacking mode to an intermixed packing mode, and to a non-stacking mode to refine its solid-state properties. Different from the above-mentioned understanding, we find that close atom contacts in a non-stacking mode can form efficient charge transport pathway through close side atom interactions. The intermixed solid-state packing motif in active layers could enable organic solar cells with superior efficiency and reduced non-radiative recombination loss compared with devices based on molecules with the classic end-group π–π stacking mode. Our observations open a new avenue in material design that endows better photovoltaic performance.
Empowering alcohols as carbonyl surrogates for Grignard-type reactions
Authors: Li Chen-Chen , Wang Haining , Sim Malcolm M. , Qiu Zihang , Chen Zhang-Pei , Khaliullin Rustam Z. , Li Chao-Jun
Published: November 2020

The Grignard reaction is a fundamental tool for constructing C-C bonds. Although it is widely used in synthetic chemistry, it is normally applied in early stage functionalizations owing to poor functional group tolerance and less availability of carbonyls at late stages of molecular modifications. Herein, we report a Grignard-type reaction with alcohols as carbonyl surrogates by using a ruthenium(II) PNP-pincer complex as catalyst. This transformation proceeds via a carbonyl intermediate generated in situ from the dehydrogenation of alcohols, which is followed by a Grignard-type reaction with a hydrazone carbanion to form a C-C bond. The reaction conditions are mild and can tolerate a broad range of substrates. Moreover, no oxidant is involved during the entire transformation, with only H2and N2being generated as byproducts. This reaction opens up a new avenue for Grignard-type reactions by enabling the use of naturally abundant alcohols as starting materials without the need for pre-synthesizing carbonyls.
Author Correction: Chemical trends of deep levels in van der Waals semiconductors
Authors: Ci Penghong , Tian Xuezeng , Kang Jun , Salazar Anthony , Eriguchi Kazutaka , Warkander Sorren , Tang Kechao , Liu Jiaman , Chen Yabin , Tongay Sefaattin , Walukiewicz Wladek , Miao Jianwei , Dubon Oscar , Wu Junqiao
Published: November 2020

A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-20151-x
Publisher Correction: HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains
Authors: Francis Ashwanth C. , Marin Mariana , Singh Parmit K. , Achuthan Vasudevan , Prellberg Mathew J. , Palermino-Rowland Kristina , Lan Shuiyun , Tedbury Philip R. , Sarafianos Stefan G. , Engelman Alan N. , Melikyan Gregory B.
Published: November 2020

A Correction to this paper has been published: https://doi.org/10.1038/s41467-020-20152-w
Multi-directionally wrinkle-able textile OLEDs for clothing-type displays
Authors: Choi Seungyeop , Jo Woosung , Jeon Yongmin , Kwon Seonil , Kwon Jeong Hyun , Son Young Hyun , Kim Junmo , Park Jun Hong , Kim Hyuncheol , Lee Ho Seung , Nam Minwoo , Jeong Eun Gyo , Bin Shin Jeong , Kim Taek-Soo , Choi Kyung Cheol
Published: November 2020

A clothing-type wearable display can be utilized in fashion, bio-healthcare, and safety industries as well as smart textiles for the internet of things (IoTs) and wearable devices. In response to this trend, we demonstrate a textile display that can endure the active movements of a human body. It can be applied to any kind of textile, and is durable against conditions such as rain, sweat, and washing. As a key technology for realizing the multi-directional wrinkle-able textile display, we fabricated a stress-lowering textile platform with an ultrathin planarization layer replicated from the flat surface of glass. An elastomeric strain buffer for reducing mechanical stress is also inserted into the textile platform. Here, organic light-emitting diodes (OLEDs) with red, green and blue color, thin film transistors (TFTs) fabricated at a low temperature below 150 °C, and a washable encapsulation layer blocking both gas and liquid were demonstrated on the textile platform.
Australian long-finned pilot whales (Globicephala melas) emit stereotypical, variable, biphonic, multi-component, and sequenced vocalisations, similar to those recorded in the northern hemisphere
Authors: Courts Rachael , Erbe Christine , Wellard Rebecca , Boisseau Oliver , Jenner K. Curt , Jenner Micheline-N.
Published: November 2020

While in the northern hemisphere, many studies have been conducted on the vocal repertoire of long-finned pilot whales (Globicephala melas), no such study has been conducted in the southern hemisphere. Presented here, is the first study on the vocalisations of long-finned pilot whales along the southern coast of mainland Australia. Multiple measures were taken of 2028 vocalisations recorded over five years in several locations. These vocalisations included tonal sounds with and without overtones, sounds of burst-pulse character, graded sounds, biphonations, and calls of multiple components. Vocalisations were further categorised based on spectrographic features into 18 contour classes. Altogether, vocalisations ranged from approximately 200 Hz to 25 kHz in fundamental frequency and from 0.03 s to 2.07 s in duration. These measures compared well with those from northern hemisphere pilot whales. Some call types were almost identical to northern hemisphere vocalisations, even though the geographic ranges of the two populations are far apart. Other call types were unique to Australia. Striking similarities with calls of short-finned pilot whales (Globicephala macrorhynchus) and sometimes sympatric killer whales (Orcinus orca) were also found. Theories for call convergence and divergence are discussed.
The implications of thumb movements for Neanderthal and modern human manipulation
Authors: Bardo Ameline , Moncel Marie-Hélène , Dunmore Christopher J. , Kivell Tracy L. , Pouydebat Emmanuelle , Cornette Raphaël
Published: November 2020

Much research has debated the technological abilities of Neanderthals relative to those of early modern humans, with a particular focus on subtle differences in thumb morphology and how this may reflect differences in manipulative behaviors in these two species. Here, we provide a novel perspective on this debate through a 3D geometric morphometric analysis of shape covariation between the trapezial and proximal first metacarpal articular surfaces of Neanderthals (Homo neanderthalensis) in comparison to early and recent humans (Homo sapiens). Results show a distinct pattern of shape covariation in Neanderthals, consistent with more extended and adducted thumb postures that may reflect habitual use of grips commonly used for hafted tools. Both Neanderthals and recent humans demonstrate high intraspecific variation in shape covariation. This intraspecific variation is likely the result of genetic and/or developmental differences, but may also reflect, in part, differing functional requirements imposed by the use of varied tool-kits. These results underscore the importance of holistic joint shape analysis for understanding the functional capabilities and evolution of the modern human thumb.
Optical imaging of muons
Authors: Yamamoto Seiichi , Ninomiya Kazuhiko , Kawamura Naritoshi , Hirano Yoshiyuki
Published: November 2020

Optical imaging of particle beams is a promising method for range and width estimations. However it was not clear that optical imaging was possible for muons. To clarify this, we conducted optical imaging of muons, since high-intensity muons are now available at J-PARC. We irradiated positive muons with different momenta to water or plastic scintillator block, and imaged using a charge-coupled device (CCD) camera during irradiation. The water and plastic scintillator block produced quite different images. The images of water during irradiation of muons produced elliptical shape light distribution at the end of the ranges due to Cherenkov-light from the positrons produced by positive muon decay, while, for the plastic scintillator block, we measured images similar to the dose distributions. We were able to estimate the ranges of muons as well as the measurement of the asymmetry of the direction of the positron emission by the muon decays from the optical images of the water, although the measured ranges were 4 mm to 5 mm larger than the calculated values. The ranges and widths of the beams could also be estimated from the optical images of the plastic scintillator block. We confirmed that optical imaging of muons was possible and is a promising method for the quality assessment, research of muons, and the future muon radiotherapy.
Facial expressions contribute more than body movements to conversational outcomes in avatar-mediated virtual environments
Authors: Oh Kruzic Catherine , Kruzic David , Herrera Fernanda , Bailenson Jeremy
Published: November 2020

This study focuses on the individual and joint contributions of two nonverbal channels (i.e., face and upper body) in avatar mediated-virtual environments. 140 dyads were randomly assigned to communicate with each other via platforms that differentially activated or deactivated facial and bodily nonverbal cues. The availability of facial expressions had a positive effect on interpersonal outcomes. More specifically, dyads that were able to see their partner’s facial movements mapped onto their avatars liked each other more, formed more accurate impressions about their partners, and described their interaction experiences more positively compared to those unable to see facial movements. However, the latter was only true when their partner’s bodily gestures were also available and not when only facial movements were available. Dyads showed greater nonverbal synchrony when they could see their partner’s bodily and facial movements. This study also employed machine learning to explore whether nonverbal cues could predict interpersonal attraction. These classifiers predicted high and low interpersonal attraction at an accuracy rate of 65%. These findings highlight the relative significance of facial cues compared to bodily cues on interpersonal outcomes in virtual environments and lend insight into the potential of automatically tracked nonverbal cues to predict interpersonal attitudes.
No relationship between the acromiohumeral distance and pain in adults with subacromial pain syndrome: a systematic review and meta-analysis
Authors: Park Soo Whan , Chen Yuan Tai , Thompson Lindsay , Kjoenoe Andreas , Juul-Kristensen Birgit , Cavalheri Vinicius , McKenna Leanda
Published: November 2020

To determine whether subacromial space (i.e. acromiohumeral distance; AHD, and/or occupation ratio percentage) differs between people with subacromial pain syndrome (SAPS) and those without. To investigate whether there is a correlation between subacromial space and pain or disability in adults with SAPS and whether temporal changes in pain or disability are accompanied by changes in subacromial space. Systematic review and meta-analysis. Fifteen studies with a total of 775 participants were included. Twelve studies were of high quality and three studies were of moderate quality using the modified Black and Downs checklist. There was no between group difference in AHD in neutral shoulder position (mean difference [95% CI] 0.28 [−0.13 to 0.69] mm), shoulder abduction at 45° (−0.02 [−0.99 to 0.96] mm) or 60° (−0.20 [−0.61 to 0.20] mm). Compared to the control group, a greater occupation ratio in neutral shoulder position was demonstrated in participants with SAPS (5.14 [1.87 to 8.4] %). There was no consistent pattern regarding the correlation between AHD and pain or disability in participants with SAPS, and no consistent increase in subacromial space with improvement in pain or disability over time. The results suggest that surgical (e.g. sub-acromial decompression) and non-surgical (e.g. manual therapy, taping, stretching and strengthening) management of subacromial pain syndrome should not focus solely on addressing a potential decrease in subacromial space, but also on the importance of other biopsychosocial factors.
Development, implementation and outcomes of a quality assurance system for the provision of continuous renal replacement therapy in the intensive care unit
Authors: Ruiz Eloy F. , Ortiz-Soriano Victor M. , Talbott Monica , Klein Bryan A. , Thompson Bastin Melissa L. , Mayer Kirby P. , Price Emily B. , Dorfman Robert , Adams Brandi N. , Fryman Lisa , Neyra Javier A. ,   The University of Kentucky CRRT Quality Assurance Group, Elias Madona , Taylor Mark , McHatton Josh , Aycinena Juan Carlos , Diaz Angel M. , Mason Stacy A. , Perkins Megan A. , Sawaya B. Peter , Fedder Kelly R. , Hornsby Amanda , Noell Natalie , Tribble Thomas A. , Kouns Jillian M.
Published: November 2020

Critically ill patients with requirement of continuous renal replacement therapy (CRRT) represent a growing intensive care unit (ICU) population. Optimal CRRT delivery demands continuous communication between stakeholders, iterative adjustment of therapy, and quality assurance systems. This Quality Improvement (QI) study reports the development, implementation and outcomes of a quality assurance system to support the provision of CRRT in the ICU. This study was carried out at the University of Kentucky Medical Center between September 2016 and June 2019. We implemented a quality assurance system using a step-wise approach based on the (a) assembly of a multidisciplinary team, (b) standardization of the CRRT protocol, (c) creation of electronic CRRT flowsheets, (d) selection, monitoring and reporting of quality metrics of CRRT deliverables, and (e) enhancement of education. We examined 34-month data comprising 1185 adult patients on CRRT (~ 7420 patient-days of CRRT) and tracked selected QI outcomes/metrics of CRRT delivery. As a result of the QI interventions, we increased the number of multidisciplinary experts in the CRRT team and ensured a continuum of education to health care professionals. We maximized to 100% the use of continuous veno-venous hemodiafiltration and doubled the percentage of patients using regional citrate anticoagulation. The delivered CRRT effluent dose (~ 30 ml/kg/h) and the delivered/prescribed effluent dose ratio (~ 0.89) remained stable within the study period. The average filter life increased from 26 to 31 h (p = 0.020), reducing the mean utilization of filters per patient from 3.56 to 2.67 (p = 0.054) despite similar CRRT duration and mortality rates. The number of CRRT access alarms per treatment day was reduced by 43%. The improvement in filter utilization translated into ~ 20,000 USD gross savings in filter cost per 100-patient receiving CRRT. We satisfactorily developed and implemented a quality assurance system for the provision of CRRT in the ICU that enabled sustainable tracking of CRRT deliverables and reduced filter resource utilization at our institution.
Impairment of motor coordination and interneuron migration in perinatal exposure to glufosinate-ammonium
Authors: Kim Kyung-Tai , Kwak Ye-Jung , Han Su-Cheol , Hwang Jeong Ho
Published: November 2020

Glufosinate-ammonium (GLA) is a broad-spectrum herbicide for agricultural weed control and crop desiccation. Due to many GLA-resistant crops being developed to effectively control weeds and increase harvest yields, herbicide usage and the residual GLA in food has increased significantly. Though perinatal exposure by the residual GLA in food might affect brain development, the developmental neurotoxicity of GLA is still unclear. Therefore, this study aimed to investigate the effects of perinatal exposure to GLA on cortical development. The analysis revealed that perinatal GLA exposure altered behavioral changes in offspring, especially motor functional behavior. Moreover, perinatal GLA exposure affected cortical development, particularly by disrupting interneuron migration. These results provide new evidence that early life exposure to GLA alters cortical development.
Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer
Authors: Spielmann Julia , Mattheis Laura , Jung Juliane-Susanne , Rauße Henrik , Glaß Markus , Bähr Ina , Quandt Dagmar , Oswald Jana , Kielstein Heike
Published: November 2020

Obesity is a widely spread disease and a crucial risk factor for malign disorders, including breast cancer of women in the postmenopause. Studies demonstrated that in case of obesity crucial natural killer (NK) cell functions like combating tumor cells are affected. This study aims to analyze NK cells and NK cell receptor expression of obese mice in a model for postmenopausal breast cancer. Therefore, female BALB/c mice were fed either a high fat or a standard diet. Thereafter, ovaries were ectomized and a syngeneic and orthotopical injection of 4T1-luc2 mouse mammary tumor cells into the mammary adipose tissue pad was performed. Obese mice showed increased body weights and visceral fat mass as well as increased levels of leptin and IL-6 in plasma. Moreover, compared to the lean littermates, tumor growth was increased and the NKp46-expression on circulating NK cells was decreased. Furthermore, the activating NK cell receptor NKG2D ligand (MULT1) expression was enhanced in adipose tissue of obese tumor bearing mice. The present study gives novel insights into gene expression of NK cell receptors in obesity and aims to promote possible links of the obesity-impaired NK cell physiology and the elevated breast cancer risk in obese women.
Phenotypic expression and clinical outcomes in a South Asian PRKAG2 cardiomyopathy cohort
Authors: Ahamed Hisham , Balegadde Aniketh Vijay , Menon Shilpa , Menon Ramesh , Ramachandran Aishwarya , Mathew Navin , Natarajan K. U. , Nair Indu Ramachandran , Kannan Rajesh , Shankar Meghna , Mathew Oommen K. , Nguyen Thong T. , Gupta Ravi , Stawiski Eric W. , Ramprasad V. L. , Seshagiri Somasekar , Phalke Sameer
Published: November 2020

The PRKAG2 syndrome is a rare autosomal dominant phenocopy of sarcomeric hypertrophic cardiomyopathy (HCM), characterized by ventricular pre-excitation, progressive conduction system disease and left ventricular hypertrophy. This study describes the phenotype, genotype and clinical outcomes of a South-Asian PRKAG2 cardiomyopathy cohort over a 7-year period. Clinical, electrocardiographic, echocardiographic, and cardiac MRI data from 22 individuals with PRKAG2 variants (68% men; mean age 39.5 ± 18.1 years), identified at our HCM centre were studied prospectively. At initial evaluation, all of the patients were in NYHA functional class I or II. The maximum left ventricular wall thickness was 22.9 ± 8.7 mm and left ventricular ejection fraction was 53.4 ± 6.6%. Left ventricular hypertrophy was present in 19 individuals (86%) at baseline. 17 patients had an WPW pattern (77%). After a mean follow-up period of 7 years, 2 patients had undergone accessory pathway ablation, 8 patients (36%) underwent permanent pacemaker implantation (atrio-ventricular blocks—5; sinus node disease—2), 3 patients developed atrial fibrillation, 11 patients (50%) developed progressive worsening in NYHA functional class, and 6 patients (27%) experienced sudden cardiac death or equivalent. PRKAG2 cardiomyopathy must be considered in patients with HCM and progressive conduction system disease.
Identification and motif analyses of candidate nonreceptor olfactory genes of Dendroctonus adjunctus Blandford (Coleoptera: Curculionidae) from the head transcriptome
Authors: Torres-Huerta Brenda , Segura-León Obdulia L. , Aragón-Magadan Marco A. , González-Hernández Héctor
Published: November 2020

The round-headed pine beetle Dendroctonus adjunctus, whose dispersion and colonization behaviors are linked to a communication system mediated by semiochemicals, is one of the five most critical primary pests in forest ecosystems in Mexico. This study provides the first head transcriptome analysis of D. adjunctus and the identification of the nonreceptor olfactory genes involved in the perception of odors. De novo assembly yielded 44,420 unigenes, and GO annotations were similar to those of antennal transcriptomes of other beetle species, which reflect metabolic processes related to smell and signal transduction. A total of 36 new transcripts of nonreceptor olfactory genes were identified, of which 27 encode OBPs, 7 encode CSPs, and 2 encode SNMP candidates, which were subsequently compared to homologous proteins from other bark beetles and Coleoptera species by searching for sequence motifs and performing phylogenetic analyses. Our study provides information on genes encoding nonreceptor proteins in D. adjunctus and broadens the knowledge of olfactory genes in Coleoptera and bark beetle species, and will help to understand colonization and aggregation behaviors for the development of tools that complement management strategies.
3D bioprinting of bicellular liver lobule-mimetic structures via microextrusion of cellulose nanocrystal-incorporated shear-thinning bioink
Authors: Wu Yun , Wenger Andrew , Golzar Hossein , Tang Xiaowu
Published: November 2020

3D bioprinting of living cellular constructs with heterogeneity in cell types and extra cellular matrices (ECMs) matching those of biological tissues remains challenging. Here, we demonstrate that, through bioink material design, microextrusion-based (ME) bioprinting techniques have the potential to address this challenge. A new bioink employing alginate (1%), cellulose nanocrystal (CNC) (3%), and gelatin methacryloyl (GelMA) (5%) (namely 135ACG hybrid ink) was formulated for the direct printing of cell-laden and acellular architectures. The 135ACG ink displayed excellent shear-thinning behavior and solid-like properties, leading to high printability without cell damage. After crosslinking, the ACG gel can also provide a stiff ECM ideal for stromal cell growth. By controlling the degree of substitution and polymer concentration, a GelMA (4%) bioink was designed to encapsulate hepatoma cells (hepG2), as GelMA gel possesses the desired low mechanical stiffness matching that of human liver tissue. Four different versions of to-scale liver lobule-mimetic constructs were fabricated via ME bioprinting, with precise positioning of two different cell types (NIH/3T3 and hepG2) embedded in matching ECMs (135ACG and GelMA, respectively). The four versions allowed us to exam effects of mechanical cues and intercellular interactions on cell behaviors. Fibroblasts thrived in stiff 135ACG matrix and aligned at the 135ACG/GelMA boundary due to durotaxis, while hepG2 formed spheroids exclusively in the soft GelMA matrix. Elevated albumin production was observed in the bicellular 3D co-culture of hepG2 and NIH/3T3, both with and without direct intercellular contact, indicating that improved hepatic cell function can be attributed to soluble chemical factors. Overall, our results showed that complex constructs with multiple cell types and varying ECMs can be bioprinted and potentially useful for both fundamental biomedical research and translational tissue engineering.
Genetic origins and diversity of bushpigs from Madagascar (Potamochoerus larvatus, family Suidae)
Authors: Lee Carol , Day Jenna , Goodman Steven M. , Pedrono Miguel , Besnard Guillaume , Frantz Laurent , Taylor Peter J. , Herrera Michael J. , Gongora Jaime
Published: November 2020

The island of Madagascar, situated off the southeast coast of Africa, shows the first evidence of human presence ~ 10,000 years ago; however, other archaeological data indicates a settlement of the modern peoples of the island distinctly more recent, perhaps > 1500 years ago. Bushpigs of the genusPotamochoerus(family Suidae), are today widely distributed in Madagascar and presumed to have been introduced from Africa at some stage by human immigrants to the island. However, disparities about their origins in Madagascar have been presented in the literature, including the possibility of endemic subspecies, and few empirical data are available. Furthermore, the separation of bushpigs in Madagascar from their mainland relatives may have favoured the evolution of a different repertoire of immune genes first due to a founder effect and then as a response to distinct pathogens compared to their ancestors. Molecular analysis confirmed the species status of the bushpig in Madagascar asP. larvatus, likely introduced from the central region of southern Africa, with no genetic evidence for the recognition of eastern and western subspecies as suggested from previous cranial morphology examination. Investigation of the immunologically importantSLA-DQB1peptide-binding region showed a different immune repertoire of bushpigs in Madagascar compared to those on the African mainland, with seventeen exon-2 haplotypes unique to bushpigs in Madagascar (2/28 haplotypes shared). This suggests that the MHC diversity of the Madagascar populations may have enabled Malagasy bushpigs to adapt to new environments.
Evaluation of the upper airway microbiome and immune response with nasal epithelial lining fluid absorption and nasal washes
Authors: Shilts Meghan H. , Rosas-Salazar Christian , Lynch Christian E. , Tovchigrechko Andrey , Boone Helen H. , Russell Patty B. , Connolly Alexandra S. , Costello Kaitlin M. , McCollum Megan D. , Mai Annie , Wiggins Derek A. , Rajagopala Seesandra V. , Yooseph Shibu , Peebles R. Stokes , Hartert Tina V. , Das Suman R.
Published: November 2020

Despite being commonly used to collect upper airway epithelial lining fluid, nasal washes are poorly reproducible, not suitable for serial sampling, and limited by a dilution effect. In contrast, nasal filters lack these limitations and are an attractive alternative. To examine whether nasal filters are superior to nasal washes as a sampling method for the characterization of the upper airway microbiome and immune response, we collected paired nasal filters and washes from a group of 40 healthy children and adults. To characterize the upper airway microbiome, we used 16S ribosomal RNA and shotgun metagenomic sequencing. To characterize the immune response, we measured total protein using a BCA assay and 53 immune mediators using multiplex magnetic bead-based assays. We conducted statistical analyses to compare common microbial ecology indices and immune-mediator median fluorescence intensities (MFIs) between sample types. In general, nasal filters were more likely to pass quality control in both children and adults. There were no significant differences in microbiome community richness, α-diversity, or structure between pediatric samples types; however, these were all highly dissimilar between adult sample types. In addition, there were significant differences in the abundance of amplicon sequence variants between sample types in children and adults. In adults, total proteins were significantly higher in nasal filters than nasal washes; consequently, the immune-mediator MFIs were not well detected in nasal washes. Based on better quality control sequencing metrics and higher immunoassay sensitivity, our results suggest that nasal filters are a superior sampling method to characterize the upper airway microbiome and immune response in both children and adults.
Sustained spatial attention accounts for the direction bias of human microsaccades
Authors: Xue Cheng , Calapai Antonino , Krumbiegel Julius , Treue Stefan
Published: November 2020

Small ballistic eye movements, so called microsaccades, occur even while foveating an object. Previous studies using covert attention tasks have shown that shortly after a symbolic spatial cue, specifying a behaviorally relevant location, microsaccades tend to be directed toward the cued location. This suggests that microsaccades can serve as an index for the covert orientation of spatial attention. However, this hypothesis faces two major challenges: First, effects associated with visual spatial attention are hard to distinguish from those that associated with the contemplation of foveating a peripheral stimulus. Second, it is less clear whether endogenously sustained attention alone can bias microsaccade directions without a spatial cue on each trial. To address the first issue, we investigated the direction of microsaccades in human subjects while they attended to a behaviorally relevant location and prepared a response eye movement either toward or away from this location. We find that directions of microsaccades are biased toward the attended location rather than towards the saccade target. To tackle the second issue, we verbally indicated the location to attend before the start of each block of trials, to exclude potential visual cue-specific effects on microsaccades. Our results indicate that sustained spatial attention alone reliably produces the microsaccade direction effect. Overall, our findings demonstrate that sustained spatial attention alone, even in the absence of saccade planning or a spatial cue, is sufficient to explain the direction bias observed in microsaccades.
Trends in global dependency on the Indonesian palm oil and resultant environmental impacts
Authors: Shigetomi Yosuke , Ishimura Yuichi , Yamamoto Yuki
Published: November 2020

Rapid growth in the international demand for palm oil has triggered considerable global concern because oil palm plantations deteriorate the environment where they are developed, resulting in complex environmental impacts in the producer nations. Here, we illustrate the historical trends in the structure of Indonesian palm oil supply chains and how these have been affected by the final demand of other nations since 2000 by using the most recent dataset of global material flows of palm oil and a global input–output database. In addition, the combination of spatial land-use change with palm oil consumption along the supply chains illustrates the linkages between ultimate consumption and land-use changes due to the palm oil plantations. As a result, the major contributors to palm oil production in Indonesia were mostly stable, being India, China, Western Europe, the United States, and Japan. However, the contribution of Indonesia declined by 6% during 2000–2013, illustrating a possible shift towards palm oil being used for non-food demands, such as apparel and medicines. Building on consumption-based accounting schemes as demonstrated by this study are considered necessary to protect local ecosystems and society.
Combining HR-TEM and XPS to elucidate the core–shell structure of ultrabright CdSe/CdS semiconductor quantum dots
Authors: Weigert Florian , Müller Anja , Häusler Ines , Geißler Daniel , Skroblin Dieter , Krumrey Michael , Unger Wolfgang , Radnik Jörg , Resch-Genger Ute
Published: November 2020

Controlling thickness and tightness of surface passivation shells is crucial for many applications of core–shell nanoparticles (NP). Usually, to determine shell thickness, core and core/shell particle are measured individually requiring the availability of both nanoobjects. This is often not fulfilled for functional nanomaterials such as many photoluminescent semiconductor quantum dots (QD) used for bioimaging, solid state lighting, and display technologies as the core does not show the application-relevant functionality like a high photoluminescence (PL) quantum yield, calling for a whole nanoobject approach. By combining high-resolution transmission electron microscopy (HR-TEM) and X-ray photoelectron spectroscopy (XPS), a novel whole nanoobject approach is developed representatively for an ultrabright oleic acid-stabilized, thick shell CdSe/CdS QD with a PL quantum yield close to unity. The size of this spectroscopically assessed QD, is in the range of the information depth of usual laboratory XPS. Information on particle size and monodispersity were validated with dynamic light scattering (DLS) and small angle X-ray scattering (SAXS) and compared to data derived from optical measurements. In addition to demonstrating the potential of this novel whole nanoobject approach for determining architectures of small nanoparticles, the presented results also highlight challenges faced by different sizing and structural analysis methods and method-inherent uncertainties.
A systematic review and meta-analysis of effects of menopausal hormone therapy on cardiovascular diseases
Authors: Kim Ji-Eun , Chang Jae-Hyuck , Jeong Min-Ji , Choi Jaesung , Park JooYong , Baek Chaewon , Shin Aesun , Park Sang Min , Kang Daehee , Choi Ji-Yeob
Published: November 2020

A systematic review and meta-analysis of randomized controlled trials (RCTs) and observational studies was conducted to assess the association between menopausal hormone therapy and cardiovascular disease. The PubMed and EMBASE databases were searched for articles published from 2000 to 2019, using review methods based on a previous Cochrane review. Quality assessment of RCTs and observational studies was conducted using the Jadad scale and the Newcastle–Ottawa Scale, respectively. A total of 26 RCTs and 47 observational studies were identified. The study populations in the RCTs were older and had more underlying diseases than those in the observational studies. Increased risks of venous thromboembolism [summary estimate (SE), 95% confidence interval (CI): RCTs, 1.70, 1.33–2.16; observational studies, 1.32, 1.13–1.54] were consistently identified in both study types, whereas an increased risk of stroke in RCTs (SE: 1.14, 95% CI: 1.04–1.25) and a decreased risk of myocardial infarction in observational studies (SE: 0.79, 95% CI: 0.75–0.84) were observed. Differential clinical effects depending on timing of initiation, underlying disease, regimen type, and route of administration were identified through subgroup analyses. These findings suggest that underlying disease and timing of initiation should be carefully considered before starting therapy in postmenopausal women.
Redesigned reporter gene for improved proton exchange-based molecular MRI contrast
Authors: Perlman Or , Ito Hirotaka , Gilad Assaf A. , McMahon Michael T. , Chiocca E. Antonio , Nakashima Hiroshi , Farrar Christian T.
Published: November 2020

Reporter gene imaging allows for non-invasive monitoring of molecular processes in living cells, providing insights on the mechanisms underlying pathology and therapy. A lysine-rich protein (LRP) chemical exchange saturation transfer (CEST) MRI reporter gene has previously been developed and used to image tumor cells, cardiac viral gene transfer, and oncolytic virotherapy. However, the highly repetitive nature of the LRP reporter gene sequence leads to DNA recombination events and the expression of a range of truncated LRP protein fragments, thereby greatly limiting the CEST sensitivity. Here we report the use of a redesigned LRP reporter (rdLRP), aimed to provide excellent stability and CEST sensitivity. The rdLRP contains no DNA repeats or GC rich regions and 30% less positively charged amino-acids. RT-PCR of cell lysates transfected with rdLRP demonstrated a stable reporter gene with a single distinct band corresponding to full-length DNA. A distinct increase in CEST-MRI contrast was obtained in cell lysates of rdLRP transfected cells and in in vivo LRP expressing mouse brain tumors ($$p=0.0275$$

p
=
0.0275

, n = 10).
Empty mesoporous silica particles significantly delay disease progression and extend survival in a mouse model of ALS
Authors: Leyton-Jaimes Marcel F. , Ivert Patrik , Hoeber Jan , Han Yilin , Feiler Adam , Zhou Chunfang , Pankratova Stanislava , Shoshan-Barmatz Varda , Israelson Adrian , Kozlova Elena N.
Published: November 2020

Amyotrophic lateral sclerosis (ALS) is a devastating incurable neurological disorder characterized by motor neuron (MN) death and muscle dysfunction leading to mean survival time after diagnosis of only 2–5 years. A potential ALS treatment is to delay the loss of MNs and disease progression by the delivery of trophic factors. Previously, we demonstrated that implanted mesoporous silica nanoparticles (MSPs) loaded with trophic factor peptide mimetics support survival and induce differentiation of co-implanted embryonic stem cell (ESC)-derived MNs. Here, we investigate whether MSP loaded with peptide mimetics of ciliary neurotrophic factor (Cintrofin), glial-derived neurotrophic factor (Gliafin), and vascular endothelial growth factor (Vefin1) injected into the cervical spinal cord of mutant SOD1 mice affect disease progression and extend survival. We also transplanted boundary cap neural crest stem cells (bNCSCs) which have been shown previously to have a positive effect on MN survival in vitro and in vivo. We show that mimetic-loaded MSPs and bNCSCs significantly delay disease progression and increase survival of mutant SOD1 mice, and also that empty particles significantly improve the condition of ALS mice. Our results suggest that intraspinal delivery of MSPs is a potential therapeutic approach for the treatment of ALS.
Integration of a soft dielectric composite into a cantilever beam for mechanical energy harvesting, comparison between capacitive and triboelectric transducers
Authors: Pruvost Mickaël , Smit Wilbert J. , Monteux Cécile , Del Corro Pablo , Dufour Isabelle , Ayela Cédric , Poulin Philippe , Colin Annie
Published: November 2020

Flexible dielectrics that harvest mechanical energy via electrostatic effects are excellent candidates as power sources for wearable electronics or autonomous sensors. The integration of a soft dielectric composite (polydimethylsiloxane PDMS-carbon black CB) into two mechanical energy harvesters is here presented. Both are based on a similar cantilever beam but work on different harvesting principles: variable capacitor and triboelectricity. We show that without an external bias the triboelectric beam harvests a net density power of 0.3 $$\upmu \mathrm{W}/{\mathrm{cm}}^{2}$$

μ
W
/

cm

2

under a sinusoidal acceleration of 3.9g at 40 Hz. In a variable capacitor configuration, a bias of 0.15 $$\mathrm{V}/\upmu \mathrm{m}$$

V
/
μ
m

is required to get the same energy harvesting performance under the same working conditions. As variable capacitors’ harvesting performance are quadratically dependent on the applied bias, increasing the bias allows the system to harvest energy much more efficiently than the triboelectric one. The present results make CB/PDMS composites promising for autonomous portable multifunctional systems and intelligent sensors.
Fibrinolysis-resistant carbonylated fibrin detected in thrombi attached to the vascular wall of abdominal aortic aneurysms
Authors: Suzuki Yuko , Tanaka Hiroki , Horinouchi Takahiro , Sano Hideto , Honkura Naoki , Unno Naoki , Miwa Soichi , Urano Tetsumei
Published: November 2020

In this study, we investigated how carbonylation of fibrinogen by acrolein modified its indispensable function to enhance fibrinolysis after being converted to fibrin and contributed to generating a fibrinolysis-resistant fibrin clot. Acrolein-treated fibrinogen was subjected to tissue plasminogen activator-induced fibrinolysis assay and the effect of lysine residue carbonylation in fibrinogen on fibrinolysis was analyzed. The acrolein-treated fibrinogen-derived fibrin clot appeared more resistant to fibrinolysis and the N-acetyl 3-formyl-3,4-dehydropiperidino (FDP)-Lysine levels in the lysed solution were positively correlated with the duration of clot lysis. The lysine analog 6-amino hexanoic acid (6AHA), which mimics the C-terminal lysine of fibrin, was carbonylated and its enhancing effect on Glu1-plasminogen activation was evaluated. After incubation with acrolein, 6AHA was converted to N-acetyl FDP-6AHA, losing its ability to enhance Glu1-plasminogen activation. These results suggest that fibrinogen carbonylation by acrolein to generate N-acetyl FDP-Lysine resulted in the generation of fibrinolysis-resistant fibrin by attenuating the C-terminal lysine-dependent activation of the Glu1-plasminogen. In abdominal aortic aneurysms, fibrin(ogen) containing the acrolein adduct N-acetyl FDP-Lysine was detected in the vascular wall-attached thrombi. These results suggest that this mechanism is likely involved in the modification of fibrinolysis-resistant thrombi and to their persistence for a long period.
Activation of p38 MAPK participates in the sulbactam-induced cerebral ischemic tolerance mediated by glial glutamate transporter-1 upregulation in rats
Authors: Xian Xiao-Hui , Gao Jun-Xia , Qi Jie , Fan Shu-Juan , Zhang Min , Li Wen-Bin
Published: November 2020

Our previous studies have shown that sulbactam can play a neuroprotection role in hippocampal neurons by upregulating the expression and function of glial glutamate transporter-1 (GLT-1) during ischemic insult. Here, using rat global cerebral ischemia model, we studied in vivo the role of p38 mitogen-activated protein kinases (MAPK) in the sulbactam-induced GLT-1 upregulation and neuroprotection against ischemia. The hippocampal CA1 field was selected as observing target. The expressions of phosphorylated-p38 MAPK and GLT-1 were assayed with western blot analysis and immunohistochemistry. The condition of delayed neuronal death (DND) was assayed with neuropathological evaluation under thionin staining. It was shown that administration of sulbactam protected CA1 hippocampal neurons against ischemic insult accompanied with significantly upregulation in the expressions of phosphorylated-p38 MAPK and GLT-1. The time course analysis showed that sulbactam activated p38 MAPK before the GLT-1 upregulation in either normal or global cerebral ischemic rats. Furthermore, inhibiting p38 MAPK activation by SB203580 blocked the GLT-1 upregulation and neuroprotection induced by sulbactam. The above results suggested that p38 MAPK, at least partly, participated in the sulbactam-induced brain tolerance to ischemia mediated by GLT-1 upregulation in rats.
Searching for the roots of the first free African American community
Authors: Martínez Beatriz , Simão Filipa , Gomes Verónica , Nguidi Masinda , Amorim Antonio , Carvalho Elizeu F. , Marrugo Javier , Gusmão Leonor
Published: November 2020

San Basilio de Palenque is an Afro-descendant community near Cartagena, Colombia, founded in the sixteenth century. The recognition of the historical and cultural importance of Palenque has promoted several studies, namely concerning the African roots of its first inhabitants. To deepen the knowledge of the origin and diversity of the Palenque parental lineages, we analysed a sample of 81 individuals for the entire mtDNA Control Region as well as 92 individuals for 27 Y-STRs and 95 for 51 Y-SNPs. The results confirmed the strong isolation of the Palenque, with some degree of influx of Native American maternal lineages, and a European admixture exclusively mediated by men. Due to the high genetic drift observed, a pairwise FST analysis with available data on African populations proved to be inadequate for determining population affinities. In contrast, when a phylogenetic approach was used, it was possible to infer the phylogeographic origin of some lineages in Palenque. Contradicting previous studies indicating a single African origin, our results evidence parental genetic contributions from widely different African regions.
Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp
Authors: Nantavisai Sirirat , Pisitkun Trairak , Osathanon Thanaphum , Pavasant Prasit , Kalpravidh Chanin , Dhitavat Sirakarnt , Makjaroen Jiradej , Sawangmake Chenphop
Published: November 2020

Utilization of canine mesenchymal stem cells (cMSCs) for regenerating incorrigible bone diseases has been introduced. However, cMSCs harvested from different sources showed distinct osteogenicity. To clarify this, comparative proteomics-based systems biology analysis was used to analyze osteogenic differentiation behavior by cMSCs harvested from bone marrow and dental pulp. The results illustrated that canine dental pulp stem cells (cDPSCs) contained superior osteogenicity comparing with canine bone marrow-derived MSCs (cBM-MSCs) regarding alkaline phosphatase activity, matrix mineralization, and osteogenic marker expression. Global analyses by proteomics platform showed distinct protein clustering and expression pattern upon an in vitro osteogenic induction between them. Database annotation using Reactome and DAVID revealed contrast and unique expression profile of osteogenesis-related proteins, particularly on signaling pathways, cellular components and processes, and cellular metabolisms. Functional assay and hierarchical clustering for tracking protein dynamic change confirmed that cBM-MSCs required the presences of Wnt, transforming growth factor (TGF)-beta, and bone-morphogenetic protein (BMP) signaling, while cDPSCs mainly relied on BMP signaling presentation during osteogenic differentiation in vitro. Therefore, these findings illustrated the comprehensive data regarding an in vitro osteogenic differentiation behavior by cBM-MSCs and cDPSCs which is crucial for further mechanism study and the establishment of cMSC-based bone tissue engineering (BTE) for veterinary practice.