bluuebunny/crossref_metadata_2025 huggingface dataset:
Brassinolide Maximized the Fruit and Oil Yield, Induced the Secondary Metabolites, and Stimulated Linoleic Acid Synthesis of Opuntia ficus-indica Oil
Authors: Atteya Amira K. G. , El-Serafy Rasha S. , El-Zabalawy Khaled M. , Elhakem Abeer , Genaidy Esmail A. E.
Published: May 2022

Prickly pear plant is widely cultivated in arid and semi-arid climates. Its fruits are rich in polyphenols, proteins, vitamin C, minerals, fatty acids, and amino acids. The oil extracted from the seeds also has a significant proportion of linoleic acid (ω6) and might be employed as a therapeutic raw material. The potential of enhancing fruit yield, increasing bioactive compounds of the fruit pulp, and improving the unsaturated fatty acid content of prickly pear oilseed by using the foliar application of brassinolide as a plant growth regulator was the main goal of this study. Prickly pear plants were foliar sprayed with a brassinolide solution at concentrations of 0, 1, 3, and 5 mg L−1. The plant performance was significantly improved following brassinolide applications, as compared with untreated plants. The plants subjected to 5 mg L−1 application exhibited 183 and 188% stimulation in the fruit yield, and 167 and 172% in the seed yield for the first and second seasons, respectively. The highest concentration of phenolic, flavonoid, protein, vitamin C, and maximum antioxidant activity in the fruit pulp was observed following 5 mg L−1 brassinolide treatment. The oil yield has been increased by 366 and 353% following brassinolide at a 5 mg L−1 level over control plants. Linoleic, oleic, and palmitic acids are the major components in prickly pear seed oil. Brassinolide foliar spraying induced an alternation in the fatty acid profile, as linoleic and oleic acids exhibited 5 and 4% higher following 5 mg L−1 application as compared with untreated plants. In conclusion, the treatment of 5 mg L−1 brassinolide improved the growth and quality of prickly pear plants by boosting fruit and seed yields, increasing active component content in the fruit pulp, improving mineral content, and increasing oil production and linoleic acid proportion.
Characterisation of the Complete Chloroplast Genomes of Seven Hyacinthus orientalis L. Cultivars: Insights into Cultivar Phylogeny
Authors: Wong Kwan-Ho , Wu Hoi-Yan , Kong Bobby Lim-Ho , But Grace Wing-Chiu , Siu Tin-Yan , Hui Jerome Ho-Lam , Shaw Pang-Chui , Lau David Tai-Wai
Published: May 2022

To improve agricultural performance and obtain potential economic benefits, an understanding of phylogenetic relationships of Hyacinthus cultivars is needed. This study aims to revisit the phylogenetic relationships of Hyacinthus cultivars using complete chloroplast genomes. Nine chloroplast genomes were de novo sequenced, assembled and annotated from seven cultivars of Hyacinthus orientalis and two Scilloideae species including Bellevalia paradoxa and Scilla siberica. The chloroplast genomes of Hyacinthus cultivars ranged from 154,458 bp to 154,641 bp, while those of Bellevalia paradoxa and Scilla siberica were 154,020 bp and 154,943 bp, respectively. Each chloroplast genome was annotated with 133 genes, including 87 protein-coding genes, 38 transfer RNA genes and 8 ribosomal RNA genes. Simple sequence repeats AAGC/CTTG and ACTAT/AGTAT were identified only in ‘Eros’, while AAATC/ATTTG were identified in all cultivars except ‘Eros’. Five haplotypes were identified based on 460 variable sites. Combined with six other previously published chloroplast genomes of Scilloideae, a sliding window analysis and a phylogenetic analysis were performed. Divergence hotspots ndhA and trnG-UGC were identified with a nucleotide diversity threshold at 0.04. The phylogenetic positions of Hyacinthus cultivars were different from the previous study using ISSR. Complete chloroplast genomes serve as new evidence in Hyacinthus cultivar phylogeny, contributing to cultivar identification, preservation and breeding.
Planting Date and Different N-Fertilization Rates Differently Modulate Agronomic and Economic Traits of a Sicilian Onion Landrace and of a Commercial Variety
Authors: Di Miceli Giuseppe , Farruggia Davide , Iacuzzi Nicolò , Bacarella Simona , La Bella Salvatore , Consentino Beppe Benedetto
Published: May 2022

The onion is one of the most widespread bulb vegetables worldwide. Onion cultivation is common in Sicily, as is the use of local genotypes. Cultivation practices are of particular interest in optimizing yield, quality, and profits. The aim of this study was to assess the agronomic response of a Sicilian landrace (Bianca Calda di Comiso, coded L1) and a commercial variety (Bianca di Maggio, coded V1) to different planting dates and N fertilization rates. An economic appraisal was also performed. The two genotypes were assessed using an experimental split-split-plot design with four levels of nitrogen rates (0, 80, 160, and 220 kg N ha−1) and two different planting dates (8 October 2005 to 9 October 2006 for the early planting date, and 27 December 2005 to 30 December 2006 for the traditional planting date). The marketable yield and production parameters were significantly influenced by the nitrogen dose: higher doses led to a higher total yield, with yield peaks above 60 t ha−1 and the marketable yield ranging from 23% to 54%. Simultaneously, decreases in the firmness (from 7% to 19%) and scale content (from 1% to 3%) were also reported. The L1 landrace showed a higher production than the V1 variety. The crop year did not significantly affect the results, and the traditional planting date appeared to be the most suitable choice in obtaining the best agronomic response. Economic analysis showed that the L1 landrace, with high-N application treatments, produced greater net benefits and marginal rates of return. Thus, the L1 landrace exposed to the highest dosages of nitrogen (160 or 220 kg ha−1) and transplanted during the traditional planting period is the best choice from agronomic and economic points of view.
Factors Driving Coordinated Development of Urban Green Economy: An Empirical Evidence from the Chengdu-Chongqing Economic Circle
Authors: Wu Sentao , Deng Xin , Qi Yanbin
Published: May 2022

Globally, all countries regard the development of economic zones around basins as the focus and main axis of national economic construction. The economic development of basin areas must consider the constraints of environmental protection, which requires local governments to adopt a coordinated development approach to the green economy. The Chengdu-Chongqing Economic Circle is located in the upper reaches of the Yangtze River Basin and will be built into a growth pole for China’s economic growth. This paper uses the panel data of 16 cities in the Chengdu-Chongqing Economic Circle from 2005 to 2019 and measures the level of coordinated development of the green economy among cities. Using fixed effect regression models, this paper identified the factors driving the coordinated development level of the urban green economy. The results show that (1) the overall trend of coordinated development of green economy in the Chengdu-Chongqing economic circle is enhanced; (2) the pulling force includes the similarity of economic agglomeration and regional openness, the resonance force includes the similarity of traffic and industrial structure, the pushing force comes from the central city; (3) in the urban agglomeration with double centers, the cooperation and competition between two “pole” cities may coexist.
Direct and Indirect Determinants of Body Mass Index in Both Major Ethnic Groups Experiencing the Nutritional Transition in Cameroon
Authors: Cohen Emmanuel , Amougou Norbert , Ponty Amandine , Guerrien Margaux , Wakenge Wakilongo , Chidumwa Glory , Said-Mohamed Rihlat , Fezeu Léopold K. , Pasquet Patrick
Published: May 2022

In the context of rapid nutritional transitions in Africa, few studies have analyzed the etiology of obesity by considering the driver pathways that predict body mass index (BMI). The aim of this study is to innovatively identify these driver pathways, including the main sociodemographic and socioecological drivers of BMI. We conducted a rural–urban quantitative study in Cameroon (n = 1106; balanced sex ratio) to explore this issue. We recruited participants and reported several sociodemographic characteristics (e.g., marital status, socioeconomic status (SES), and ethnicity). We then assessed three main socioecological drivers of BMI (body weight perception, dietary intake, and physical activity) and conducted bioanthropometric measurements. We identified several driver pathways predicting BMI. In Cameroon, Bamiléké ethnicity, higher SES, being married, and older age had positive effects on BMI through overweight valorization and/or dietary intake. Accordingly, we found that being Bamiléké, married, and middle-aged, as well as having a higher SES, were factors that constituted at-risk subgroups overexposed to drivers of obesity. As such, this study highlights the necessity of investigating the complex driver pathways that lead to obesity. Therefore, better identification of the subgroups at risk for obesity will help in developing more targeted population health policies in countries where this burden is a major public health issue.
Frequency of Metabolic Syndrome and Study of Anthropometric, Clinical and Biological Characteristics in Peri- and Postmenopausal Women in the City of Ksar El Kebir (Northern Morocco)
Authors: Harraqui Khouloud , Oudghiri Dia Eddine , Hannoun Zineb , Naceiri Mrabti Hanae , Aboulghras Sara , M. Assaggaf Hamza , S. Rajab Bodour , A. Attar Ammar , Bouyahya Abdelhakim , Bour Abdellatif
Published: May 2022

This study aimed to determine the frequency of metabolic syndrome and to identify its predictive factors in peri- and post-menopausal women in the city of Ksar El Kebir, in northern Morocco. A total of 373 peri- and post-menopausal women between 45 and 64 years old participated in the study. Metabolic syndrome was diagnosed according to the National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) definition. Body mass index (BMI) was calculated to assess the degree of obesity in women; anthropometric, clinical and biological parameters were collected during interviews. The mean ages of peri- and postmenopausal women were 48.84 ± 2.4 years and 56.65 ± 4.29 years, respectively. Postmenopausal women had higher means of anthropometric and biological parameters than peri-menopausal women. We also noted a predominance of metabolic syndrome in postmenopausal women (n = 158) compared to peri-menopausal women (n = 81). Waist circumference was the predominant marker in the subjects studied, whereas triglycerides were the lower marker. In the overall population, the incidence of metabolic syndrome and its associated factors were higher in postmenopausal women than in peri-menopausal women, from which it can be concluded that post menopause may be a predictor of metabolic syndrome.
Lessons We Have Learned Regarding Seroprevalence in High and Low SARS-CoV-2 Contexts in Greece before the Omicron Pandemic Wave
Authors: Kotsiou Ourania S. , Vavougios George D. , Papagiannis Dimitrios , Matsiatsiou Elena , Avgeri Dimitra , Fradelos Evangelos C. , Siachpazidou Dimitra I. , Perlepe Garifallia , Miziou Angeliki , Kyritsis Athanasios , Gogou Eudoxia , Kalampokas Serafim , Kalantzis Georgios , Kotsios Vaios S. , Gourgoulianis Konstantinos I.
Published: May 2022

Background: Antibody seroprevalence in rural communities remains poorly investigated. We compared the SARS-CoV-2 seroprevalence in two Greek communities in June and July 2021 after the end of the Delta-driven pandemic wave that started in November 2020. One community was affected worse than the other. Methods: The SARS-CoV-2 IgG II Quant method (Architect, Abbott, IL, USA) was used for antibody testing. Results: We found a high rate of SARS-CoV-2 seropositivity in both communities, approaching 77.5%. In the area with a higher burden of COVID-19, Malesina, seropositivity was achieved with vaccine-acquired and naturally acquired immunity, whereas in the low-burden context of Domokos, the high rates of seropositivity were achieved mainly with vaccination. Previously infected individuals were less likely to be vaccinated than previously uninfected adults. The antibody titers were significantly higher in previously infected, vaccinated participants than in unvaccinated ones. In total, 4% and 10% of the unvaccinated population were diagnosed seropositive for the first time while not knowing about the previous infection. Age and gender did not impact antibody titers in high- or low-burden contexts. Conclusions: Before the Omicron pandemic wave, herd immunity was reached in different contexts in Greece. Higher antibody titers were measured in infected vaccinated individuals than in infected unvaccinated ones.
Importance of Perception of Errors and Challenges for Improving Psychological Conditioning: Mediating Effect of Expectancy-Value Using the Phantom Model for Taekwondo Athletes
Authors: Sim Young Kyun , Kim Hak Hwan , Shin Joon Ha , Seo Eun Chul , Ha Min-Seong
Published: May 2022

Maintenance of positive psychological conditioning can be a key factor in eliciting high human performance. In particular, perception of error forms a causal relationship with challenges regarding task performance. Therefore, this study aimed to investigate the mediation effect of expectancy value in the relationship between the perception of error and challenge using the Phantom Model through quantitative research. This study analyzed the causal relationship between perception of error, expectancy value, and challenge in 423 young Taekwondo athletes. Frequency analysis, confirmatory factor analysis, correlation analysis, and structural equation modeling were performed on the collected data using Jamovi 1.0.1 and AMOS 23.0 statistical programs to verify the hypothesis. The challenge demonstrated a significant difference in relation to the perception of error. These results demonstrated that the perception of error not only directly affected one’s challenge but also explained the increased challenge by mediating expectations for success and subjective values. Hence, the positive perception of error increased the athletes’ expectancy value and challenge.
Effects of the Heart to Heart Card Game for Patients with Advanced Cancer Receiving Home-Based Palliative Care: A Clinical Randomized Controlled Trial
Authors: Du Jiayi , Fu Ling , Cui Jiaxin , An Zifen , Fang Pei , Tan Lanhui , Meng Xianmei , Yu Liping
Published: May 2022

The Heart to Heart Card Game improves psychological health outcomes in hospitalized patients with advanced cancer, but effectiveness studies for patients at home are rare. This randomized controlled study was conducted to determine the effectiveness of the Heart to Heart Card Game on patients with advanced cancer receiving home-based palliative care. Sixty-six participants were randomly assigned to the intervention group (n = 34) and control group (n = 32). The quality of life, dignity, and psychological distress were considered as outcomes, which were assessed pre-intervention and six weeks after the intervention. There was a statistical difference in the quality of life (global health statues) between the intervention group and the control group after intervention (z = 2.017, p < 0.05). A significant difference was found in the quality of life (emotional, social function), dignity (symptom distress dimension), and psychological distress in the intervention group through intragroup comparison before and after the intervention. This randomized trial showed that the Heart to Heart Card Game likely alleviates barriers to end-of-life conversations and helps patients with advanced cancer maintain a more stable mental state. This trial has been registered at the Chinese Clinical Trial Registry (registration number: ChiCTR2100049933).
Psychological Flexibility Is Associated with Parental Stress in Relatives of People with Intellectual Disabilities
Authors: Lobato David , Montesinos Francisco , Flujas-Contreras Juan M.
Published: May 2022

The objective of the study was to examine the relationship between psychological flexibility, perceived stress, and psychological heath in relatives of people with a disability diagnosis. 151 relatives completed an online questionnaire that included 6-PAQ (parental psychological flexibility), PSS (perceived stress), GHQ-12 (psychological health) and WBSI (suppression of unwanted thoughts). The results showed significant relationships between the four measured variables. A bimodal distribution was observed in the variables related to psychological flexibility. The multiple regression showed that difficulties in self as context, committed actions and defusion explain a high percentage of the variance of parental stress and general psychological health. The study provides new evidence to consider psychological flexibility as a mediating variable in psychological well-being. The presented data served as the basis for the development of an ACT-based intervention protocol and the implementation of a clinical trial for relatives of children with disabilities.
Heatwave Mortality in Summer 2020 in England: An Observational Study
Authors: Thompson Ross , Landeg Owen , Kar-Purkayastha Ishani , Hajat Shakoor , Kovats Sari , O’Connell Emer
Published: May 2022

High ambient temperatures pose a significant risk to health. This study investigates the heatwave mortality in the summer of 2020 during the SARS-CoV-2 coronavirus (COVID-19) pandemic and related countermeasures. The heatwaves in 2020 caused more deaths than have been reported since the Heatwave Plan for England was introduced in 2004. The total and cause-specific mortality in 2020 was compared to previous heatwave events in England. The findings will help inform summer preparedness and planning in future years as society learns to live with COVID-19. Heatwave excess mortality in 2020 was similar to deaths occurring at home, in hospitals, and in care homes in the 65+ years group, and was comparable to the increases in previous years (2016–2018). The third heatwave in 2020 caused significant mortality in the younger age group (0–64) which has not been observed in previous years. Significant excess mortality was observed for cardiovascular disease, respiratory disease, and Alzheimer’s and Dementia across all three heatwaves in persons aged 65+ years. There was no evidence that the heatwaves affected the proportional increase of people dying at home and not seeking heat-related health care. The most significant spike in daily mortality in August 2020 was associated with a period of high night-time temperatures. The results provide additional evidence that contextual factors are important for managing heatwave risks, particularly the importance of overheating in dwellings. The findings also suggest more action is also needed to address the vulnerability in the community and in health care settings during the acute response phase of a heatwave.
Adoption of Major Housing Adaptation Policy Innovation for Older Adults by Provincial Governments in China: The Case of Existing Multifamily Dwelling Elevator Retrofit Projects
Authors: Chu Yongqiang , Shen Shuguang
Published: May 2022

(1) Background: The housing environment is crucial to the health of older Chinese people and is becoming an urgent policy initiative. This study explores factors that facilitate or impede the adoption of policy innovation on major housing adaptation (HA) by Chinese provincial governments using the framework of policy innovation and diffusion theory. (2) Methods: This study constructs an event history dataset on HA policy related to elevator retrofitting in existing multifamily dwellings in China; the lack of elevators constitutes an insurmountable barrier in older adults’ daily lives in China. The hypotheses were tested by using a traditional event history analysis (EHA) model and a piecewise constant exponential (PCE) model, which is a modified EHA model. The dataset was summarized as “province-year” event history data on 30 Chinese provinces from 2008 to 2019. (3) Results: In addition to internal determinants (e.g., population aging level and financial dependency), diffusion mechanisms can significantly facilitate or impede the adoption of major HA policy innovation by provincial governments. Policy adoption by neighboring governments helps facilitate policy adoption by nonadopters, but policy adoption by subordinate city governments impedes provincial governments’ adoption of major HA policy innovation. (4) Conclusions: This study concludes that provincial governments’ adoption of major HA policy innovation should be given a higher policy priority. The central government can promote provincial governments’ adoption of major HA that primarily benefits older adults by using fiscal transfer payments and enhancing the legitimacy of such policy.
The Elemental Characteristics and Human Health Risk of PM2.5 during Haze Episode and Non-Haze Episode in Chiang Rai Province, Thailand
Authors: Niampradit Sarima , Kliengchuay Wissanupong , Mingkhwan Rachaneekorn , Worakhunpiset Suwalee , Kiangkoo Nuttapohn , Sudsandee Suntorn , Hongthong Anuttara , Siriratruengsuk Weerayuth , Muangsuwan Thunyaluk , Tantrakarnapa Kraichat
Published: May 2022

Fine particle matter (PM2.5) was directly related to seasonal weather, and has become the influencing factor of air quality that is harmful for human health in Chiang Rai province. The aims were determining the elemental composition in PM2.5 and human health risk in haze (March 2021) and non-haze episodes (July–August 2021). Nine elements in PM2.5 were measured by using an Atomic Absorption Spectrophotometer, and an enrichment factor was used to identify the emission source. The results showed that the average concentration of PM2.5 was 63.07 μg/m3 in haze episodes, and 25.00 μg/m3 in a non-haze episode. The maximum concentration was 116.7 μg/m3 in March. The majority of elements originated from anthropogenic sources. In haze episodes, PM2.5 mean concentration was approximately 4.2 times that of the WHO guidelines (15 μg/m3 24 h), and 1.3 times that of the Thai Ambient Air Quality Standard (50 μg/m3). The analysis of backward air mass trajectory showed that transboundary and local sources significantly influenced PM2.5 at the monitoring site in the sampling period. In the health risk assessment, the non-carcinogenic risk of Cd was the highest, with a Hazard Quotient (HQ) of 0.048, and the cancer risk of Cr was classified as the highest cancer risk, with the values of 1.29 × 10−5, higher than the minimum acceptable level.
Health Occupation and Job Satisfaction: The Impact of Psychological Capital in the Management of Clinical Psychological Stressors of Healthcare Workers in the COVID-19 Era
Authors: Caponnetto Pasquale , Platania Silvia , Maglia Marilena , Morando Martina , Gruttadauria Stefania Valeria , Auditore Roberta , Ledda Caterina , Rapisarda Venerando , Santisi Giuseppe
Published: May 2022

The COVID-19 pandemic greatly impacted global health. Frontline healthcare workers involved in the response to COVID-19 faced physical and psychological challenges that threatened their wellbeing and job satisfaction. The pandemic crisis, alongside pre-existing critical issues, exposed healthcare workers to constant emotional fatigue, creating an increased workload and vulnerability to stress. Maintaining such stress levels increased their levels of anxiety, irritability and loneliness. Evidence shows that the Psychological Capital (PsyCap) was a strong protective factor against these stressors. The aim of this study was to analyze the level of job satisfaction among health workers facing the COVID-19 pandemic. The possible antecedent factors to satisfaction and the role that PsyCap plays in preserving and fostering higher levels of job satisfaction were investigated. A total of 527 healthcare workers from different areas of Italy were recruited for the study. The results revealed that psychological stress factors have a considerable impact on job satisfaction. All four predictors (Stress Vulnerability, Anxiety Symptoms, Loneliness and Irritability) had the potential to decrease job satisfaction. Loneliness had a more significant effect than other factors assessed in this study. Moreover, the results showed how PsyCap could decrease the effects of psychological stressors on job satisfaction. Consistent with previous studies, our findings show that PsyCap could alleviate negative impacts in work-related circumstances.
Factors Influencing the Size of a Non-Traumatic Full-Thickness Rotator Cuff Tear: Focusing on Socioeconomic Factors
Authors: Kang Suk-Woong , Park Chan-Kue , Woo Seung-Hun , Kim Tae-Woo , Moon Min-Hui , Yang Ji-Hee , Choi Min-Hyeok
Published: May 2022

This study aimed to identify the risk factors for non-traumatic rotator cuff tears in Korean adult patients who underwent surgical treatment, focusing on socioeconomic factors. A retrospective study was conducted with 659 patients who were diagnosed with a full rotator cuff tear and underwent surgical treatment. The outcome variable was the rotator cuff tear size (mm), as indicated by preoperative magnetic resonance imaging. Socioeconomic variables included occupation, education level, insurance type, and residential area. Univariate analyses were used to evaluate the relation between tear size and independent variables, and multivariate regression was used to estimate the effects of socioeconomic factors on tear size after adjusting for other variables. Significant differences were found in mean tear size according to age, occupation, residence area, and symptom duration (p < 0.05) in multivariate regression analysis. Rural residents had a 2.12 mm larger tear size than urban residents. Compared to National Health Insurance patients, the tear size of Medicaid beneficiaries was significantly larger (6.79 mm) in urban areas. The larger the rotator cuff tear, the greater the risk of retear and poor shoulder function. Therefore, policy efforts are required to expand access to medical care for the vulnerable.
Effects of Climatic Factors on Diarrheal Diseases among Children below 5 Years of Age at National and Subnational Levels in Nepal: An Ecological Study
Authors: Dhimal Meghnath , Bhandari Dinesh , Karki Khem B. , Shrestha Srijan Lal , Khanal Mukti , Shrestha Raja Ram Pote , Dahal Sushma , Bista Bihungum , Ebi Kristie L. , Cissé Guéladio , Sapkota Amir , Groneberg David A.
Published: May 2022

Introduction: The incidence of diarrhea, a leading cause of morbidity and mortality in low-income countries such as Nepal, is temperature-sensitive, suggesting it could be associated with climate change. With climate change fueled increases in the mean and variability of temperature and precipitation, the incidence of water and food-borne diseases are increasing, particularly in sub-Saharan Africa and South Asia. This national-level ecological study was undertaken to provide evidence linking weather and climate with diarrhea incidence in Nepal. Method: We analyzed monthly diarrheal disease count and meteorological data from all districts, spanning 15 eco-development regions of Nepal. Meteorological data and monthly data on diarrheal disease were sourced, respectively, from the Department of Hydrology and Meteorology and Health Management Information System (HMIS) of the Government of Nepal for the period from 2002 to 2014. Time-series log-linear regression models assessed the relationship between maximum temperature, minimum temperature, rainfall, relative humidity, and diarrhea burden. Predictors with p-values < 0.25 were retained in the fitted models. Results: Overall, diarrheal disease incidence in Nepal significantly increased with 1 °C increase in mean temperature (4.4%; 95% CI: 3.95, 4.85) and 1 cm increase in rainfall (0.28%; 95% CI: 0.15, 0.41). Seasonal variation of diarrheal incidence was prominent at the national level (11.63% rise in diarrheal cases in summer (95% CI: 4.17, 19.61) and 14.5% decrease in spring (95% CI: −18.81, −10.02) compared to winter season). Moreover, the effects of temperature and rainfall were highest in the mountain region compared to other ecological regions of Nepal. Conclusion: Our study provides empirical evidence linking weather factors and diarrheal disease burden in Nepal. This evidence suggests that additional climate change could increase diarrheal disease incidence across the nation. Mountainous regions are more sensitive to climate variability and consequently the burden of diarrheal diseases. These findings can be utilized to allocate necessary resources and envision a weather-based early warning system for the prevention and control of diarrheal diseases in Nepal.
Attitudes and Beliefs of the Italian Population towards COVID-19 Vaccinations
Authors: Rania Nadia , Coppola Ilaria , Brucci Marta , Lagomarsino Francesca
Published: May 2022

Background: Despite the numerous campaigns to encourage vaccination against COVID-19, the public debate and often conflicting information have left many individuals uncertain about the decision to make on whether or not to vaccinate. Methods: This research aims to analyze the attitudes and beliefs of the Italian population towards COVID-19 and other vaccinations through a quantitative methodology. In all, 500 adults (Age M = 39.52) participated in this exploratory study with an online questionnaire conducted in April 2021. Results: most participants believe vaccination is necessary to defeat COVID-19; there is an age-related difference in getting vaccinations, and women were more afraid of unexpected future effects than men; older participants have expressed a greater willingness to pay to be vaccinated against COVID-19 (4). Conclusion: In light of these results, it is necessary to pay greater attention to the perplexity and fears expressed by the population, especially women and youth, in relation to vaccinations; in fact, it would help to achieve a wider adherence to the tools designed to contain the spread of viruses at the base of severe health crises.
Parents’ Adherence to Childhood Screening Tests and Referrals: A Retrospective Cohort Study with Randomized Sampling
Authors: Amit Aharon Anat
Published: May 2022

Routine timely examinations of well-child health are important for achieving children’s good health outcomes. Nevertheless, there is evidence of low compliance with well-child visit recommendations. The aim of the study was to examine potential factors associated with parents’ nonadherence to routine childhood screening tests and their acting on further referrals following unusual findings. A retrospective cohort study was conducted among 14,348 children born in 2016–2017 and registered at mother–child health clinics in a large city in Israel. A sample of 844 children was randomly selected. Screening tests at the age of two months and nine months were examined. A multiple logistic regression examined potential factors associated with nonadherence to screening tests and to further referral for evaluation. Lower adherence to screening tests was found among parents of nine-month-old children, but adherence was higher for nurses’ screening tests than for those of physicians. Children born in a complex delivery process, older mothers with a higher number of children, and Israeli citizens were at risk of not undergoing screening tests. Fewer children in the family and initial physician’s findings were the only explanation for acting to referrals. In order to promote children’s health outcomes and public health, health policymakers should conduct campaigns to convince parents of the importance of screening tests and of adherence to referrals with the aim of ensuring their children’s wellbeing throughout the life cycle.
Using Small Area Prevalence Survey Methods to Conduct Blood Lead Assessments among Children
Authors: Egan Kathryn B. , Dignam Timothy , Brown Mary Jean , Bayleyegn Tesfaye , Blanton Curtis
Published: May 2022

Introduction: Prevalence surveys conducted in geographically small areas such as towns, zip codes, neighborhoods or census tracts are a valuable tool for estimating the extent to which environmental risks contribute to children’s blood lead levels (BLLs). Population-based, cross-sectional small area prevalence surveys assessing BLLs can be used to establish a baseline lead exposure prevalence for a specific geographic region. Materials and Methods: The required statistical methods, biological and environmental sampling, supportive data, and fieldwork considerations necessary for public health organizations to rapidly conduct child blood lead prevalence surveys at low cost using small area, cluster sampling methodology are described. Results: Comprehensive small area prevalence surveys include partner identification, background data collection, review of the assessment area, resource availability determinations, sample size calculations, obtaining the consent of survey participants, survey administration, blood lead analysis, environmental sampling, educational outreach, follow-up and referral, data entry/analysis, and report production. Discussion: Survey results can be used to estimate the geographic distribution of elevated BLLs and to investigate inequitable lead exposures and risk factors of interest. Conclusions: Public health officials who wish to assess child and household-level blood lead data can quickly apply the data collection methodologies using this standardized protocol here to target resources and obtain assistance with these complex procedures. The standardized methods allow for comparisons across geographic areas and over time.
A Bibliometric Analysis of the Literature on Irisin from 2012–2021
Authors: Liu Jiangshan , Qi Bote , Gan Lin , Shen Yanli , Zou Yu
Published: May 2022

Irisin is a hormone-like molecule mainly released by skeletal muscles in response to exercise, which is proposed to induce the ‘browning’ of white adipose tissue. Since its identification, irisin was reported to be closely associated with many metabolic diseases, including type 2 diabetes mellitus (T2DM), obesity, cardiovascular disease (CVD), and metabolic bone diseases. In recent years, irisin has attracted increasing research interest, and numerous studies have been published in this field. Thus, it is essential to identify the current research status of irisin and measure research hotspots and possible future trends. In this study, by utilizing two visualization software named CiteSpace and VOSviewer, we analyzed 1510 Web of Science publications on irisin published from 2012 to 2021. Our results show that the number of irisin-related articles published annually has increased significantly. China participates in the most studies, followed by the United States and Turkey. Firat University, Harvard University, and Shandong University are three major institutions with larger numbers of publications. The analysis of keywords co-occurrence indicates that insulin resistance, inflammation, and circulating irisin levels in serum are the research hotspots. Apoptosis, BDNF, and osteoporosis will likely become the focus of future research related to irisin. Overall, this study may provide helpful insights for researchers to understand the current research situation and identify the potential frontiers of irisin.
PM2.5 Exposure and Health Risk Assessment Using Remote Sensing Data and GIS
Authors: Xu Dan , Lin Wenpeng , Gao Jun , Jiang Yue , Li Lubing , Gao Fei
Published: May 2022

Assessing personal exposure risk from PM2.5 air pollution poses challenges due to the limited availability of high spatial resolution data for PM2.5 and population density. This study introduced a seasonal spatial-temporal method of modeling PM2.5 distribution characteristics at a 1-km grid level based on remote sensing data and Geographic Information Systems (GIS). The high-accuracy population density data and the relative exposure risk model were used to assess the relationship between exposure to PM2.5 air pollution and public health. The results indicated that the spatial-temporal PM2.5 concentration could be simulated by MODIS images and GIS method and could provide high spatial resolution data sources for exposure risk assessment. PM2.5 air pollution risks were most serious in spring and winter, and high risks of environmental health hazards were mostly concentrated in densely populated areas in Shanghai-Hangzhou Bay, China. Policies to control the total population and pollution discharge need follow the principle of adaptation to local conditions in high-risk areas. Air quality maintenance and ecological maintenance should be carried out in low-risk areas to reduce exposure risk and improve environmental health.
Patterns of Alcohol Consumption and Use of Health Services in Spanish University Students: UniHcos Project
Authors: Romero-Rodríguez Esperanza , Amezcua-Prieto Carmen , Morales Suárez-Varela María , Ayán-Pérez Carlos , Mateos-Campos Ramona , Martín-Sánchez Vicente , Ortíz-Moncada Rocío , Redondo-Martín Susana , Alguacil Ojeda Juan , Delgado-Rodríguez Miguel , Blázquez Abellán Gemma , Alonso-Molero Jéssica , Cancela-Carral José María , Valero Juan Luis Félix , Fernández-Villa Tania
Published: May 2022

The aim of the study was to examine the association of alcohol consumption patterns (hazardous alcohol use and binge drinking) and the use of emergency services and primary care consultations in university students. An observational, descriptive, cross-sectional study was conducted at eleven Spanish universities collaborating within the uniHcos Project. University students completed an online questionnaire that assessed hazardous alcohol use and binge drinking using the AUDIT questionnaire and evaluated the use of emergency services and primary care. A descriptive analysis of the data was performed, as well as the chi-squared test and Student’s t-test and nonconditional logistic regression models to examine this association. Results: There were 10,167 participants who completed the questionnaire. The prevalence of hazardous alcohol use was 16.9% (95% CI: 16.2–17.6), while the prevalence of binge drinking was 48.8% (95% CI: 47.9–49.8). There were significant differences in the use of emergency services in those surveyed with hazardous alcohol use (p < 0.001) or binge drinking pattern (p < 0.001). However, no significant differences were observed in terms of attendance during primary care visits in individuals with hazardous alcohol use (p = 0.367) or binge drinking pattern (p = 0.755). The current study shows the association between university students with a pattern of hazardous alcohol use or binge drinking and greater use of emergency services. However, no significant association was observed between the said consumption patterns and the use of primary care services.
miR-22-3p and miR-30e-5p Are Associated with Prognosis in Cervical Squamous Cell Carcinoma
Authors: Kwon Ah-Young , Jeong Ju-Yeon , Park Hyun , Hwang Sohyun , Kim Gwangil , Kang Haeyoun , Heo Jin-Hyung , Lee Hye Jin , Kim Tae-Heon , An Hee Jung
Published: May 2022

Alteration in expression of miRNAs can cause various malignant changes and the metastatic process. Our aim was to identify the miRNAs involved in cervical squamous cell carcinoma (SqCC) and metastasis, and to test their utility as indicators of metastasis and survival. Using microarray technology, we performed miRNA expression profiling on primary cervical SqCC tissue (n = 6) compared with normal control (NC) tissue and compared SqCC that had (SqC-M; n = 3) and had not (SqC-NM; n = 3) metastasized. Four miRNAs were selected for validation by qRT-PCR on 29 SqC-NM and 27 SqC-M samples, and nine metastatic lesions (ML-SqC), from a total of 56 patients. Correlation of miRNA expression and clinicopathological parameters was analyzed to evaluate the clinical impact of candidate miRNAs. We found 40 miRNAs differentially altered in cervical SqCC tissue: 21 miRNAs were upregulated and 19 were downregulated (≥2-fold, p < 0.05). Eight were differentially altered in SqC-M compared with SqC-NM samples: four were upregulated (miR-494, miR-92a-3p, miR-205-5p, and miR-221-3p), and four were downregulated (miR-574-3p, miR-4769-3p, miR-1281, and miR-1825) (≥1.5-fold, p < 0.05). MiR-22-3p might be a metastamiR, which was gradually further downregulated in SqC-NM > SqC-M > ML-SqC. Downregulation of miR-30e-5p significantly correlated with high stage, lymph node metastasis, and low survival rate, suggesting an independent poor prognostic factor.
Correction: Pande et al. Nitric Oxide Signaling and Its Association with Ubiquitin-Mediated Proteasomal Degradation in Plants. Int. J. Mol. Sci. 2022, 23, 1657
Authors: Pande Anjali , Mun Bong-Gyu , Khan Murtaza , Rahim Waqas , Lee Da-Sol , Lee Geun-Mo , Al Azawi Tiba Nazar Ibrahim , Hussain Adil , Yun Byung-Wook
Published: May 2022

The authors wish to make the following corrections to the original publication [...]
ESR1 Methylation Measured in Cell-Free DNA to Evaluate Endocrine Resistance in Metastatic Breast Cancer Patients
Authors: Bos Manouk K. , Deger Teoman , Sleijfer Stefan , Martens John W. M. , Wilting Saskia M.
Published: May 2022

ESR1 methylation was proposed as mechanism for endocrine resistance in metastatic breast cancer patients. To evaluate its potential as a minimally invasive biomarker, we investigated the feasibility of measuring ESR1 methylation in cell-free DNA (cfDNA) and its association with endocrine resistance. First, we provided evidence that demethylation in vitro restores ER expression. Subsequently, we found that ESR1 methylation in cfDNA was not enriched in endocrine-resistant versus endocrine-sensitive patients. Interestingly, we found a correlation between ESR1 methylation and age. Publicly available data confirm an age-related increase in ESR1 methylation in leukocytes, confounding the determination of the ESR1 methylation status of tumors using cfDNA.
Association Mechanism and Conformational Changes in Trypsin on Its Interaction with Atrazine: A Multi- Spectroscopic and Biochemical Study with Computational Approach
Authors: Khayyat Arwa Ishaq A. , Zargar Seema , Wani Tanveer A. , Rehman Muneeb U. , Khan Azmat Ali
Published: May 2022

Atrazine (ATR) is a herbicide globally used to eliminate undesired weeds. Herbicide usage leads to various adverse effects on human health and the environment. The primary source of herbicides in humans is the food laced with the herbicides. The ATR binding to trypsin (TYP) was investigated in this study to explore its binding potential and toxicity. In vitro interaction of ATR with TYP was studied using multi-spectroscopic methods, molecular docking, and enzyme kinetics to explore the mechanism of binding for the TYP-ATR system. The TYP-ATR complex revealed binding constants (103 M−1), suggesting a moderate binding. The free energy for the TYP-ATR complexes was negative, suggesting a spontaneous interaction. Thermodynamic parameters enthalpy (ΔH) and entropy (ΔS) obtained positive values for the TYP-ATR system suggesting hydrophobic interactions in the binding process. Micro-environmental and conformational changes in TYP molecules were induced on interaction with ATR. Reduced catalytic activity of TYP was observed after interaction with ATR owing to the changes in the secondary structure of the TYP.
Transcriptome and Physiological Analyses of a Navel Orange Mutant with Improved Drought Tolerance and Water Use Efficiency Caused by Increases of Cuticular Wax Accumulation and ROS Scavenging Capacity
Authors: Liang Beibei , Wan Shiguo , Ma Qingling , Yang Li , Hu Wei , Kuang Liuqing , Xie Jingheng , Liu Dechun , Liu Yong
Published: May 2022

Drought is one of the main abiotic stresses limiting the quality and yield of citrus. Cuticular waxes play an important role in regulating plant drought tolerance and water use efficiency (WUE). However, the contribution of cuticular waxes to drought tolerance, WUE and the underlying molecular mechanism is still largely unknown in citrus. ‘Longhuihong’ (MT) is a bud mutant of ‘Newhall’ navel orange with curly and bright leaves. In this study, significant increases in the amounts of total waxes and aliphatic wax compounds, including n-alkanes, n-primary alcohols and n-aldehydes, were overserved in MT leaves, which led to the decrease in cuticular permeability and finally resulted in the improvements in drought tolerance and WUE. Compared to WT leaves, MT leaves possessed much lower contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), significantly higher levels of proline and soluble sugar, and enhanced superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under drought stress, which might reduce reactive oxygen species (ROS) damage, improve osmotic regulation and cell membrane stability, and finally, enhance MT tolerance to drought stress. Transcriptome sequencing results showed that seven structural genes were involved in wax biosynthesis and export, MAPK cascade, and ROS scavenging, and seven genes encoding transcription factors might play an important role in promoting cuticular wax accumulation, improving drought tolerance and WUE in MT plants. Our results not only confirmed the important role of cuticular waxes in regulating citrus drought resistance and WUE but also provided various candidate genes for improving citrus drought tolerance and WUE.
Penicillin-Binding Proteins, β-Lactamases, and β-Lactamase Inhibitors in β-Lactam-Producing Actinobacteria: Self-Resistance Mechanisms
Authors: Martin Juan F. , Alvarez-Alvarez Ruben , Liras Paloma
Published: May 2022

The human society faces a serious problem due to the widespread resistance to antibiotics in clinical practice. Most antibiotic biosynthesis gene clusters in actinobacteria contain genes for intrinsic self-resistance to the produced antibiotics, and it has been proposed that the antibiotic resistance genes in pathogenic bacteria originated in antibiotic-producing microorganisms. The model actinobacteria Streptomyces clavuligerus produces the β-lactam antibiotic cephamycin C, a class A β-lactamase, and the β lactamases inhibitor clavulanic acid, all of which are encoded in a gene supercluster; in addition, it synthesizes the β-lactamase inhibitory protein BLIP. The secreted clavulanic acid has a synergistic effect with the cephamycin produced by the same strain in the fight against competing microorganisms in its natural habitat. High levels of resistance to cephamycin/cephalosporin in actinobacteria are due to the presence (in their β-lactam clusters) of genes encoding PBPs which bind penicillins but not cephalosporins. We have revised the previously reported cephamycin C and clavulanic acid gene clusters and, in addition, we have searched for novel β-lactam gene clusters in protein databases. Notably, in S. clavuligerus and Nocardia lactamdurans, the β-lactamases are retained in the cell wall and do not affect the intracellular formation of isopenicillin N/penicillin N. The activity of the β-lactamase in S. clavuligerus may be modulated by the β-lactamase inhibitory protein BLIP at the cell-wall level. Analysis of the β-lactam cluster in actinobacteria suggests that these clusters have been moved by horizontal gene transfer between different actinobacteria and have culminated in S. clavuligerus with the organization of an elaborated set of genes designed for fine tuning of antibiotic resistance and cell wall remodeling for the survival of this Streptomyces species. This article is focused specifically on the enigmatic connection between β-lactam biosynthesis and β-lactam resistance mechanisms in the producer actinobacteria.