bluuebunny/crossref_metadata_2025 huggingface dataset:
The key to temperate savanna restoration is to increase plant species richness reasonably
Authors: Dou Pengpeng , Miao Zhengzhou , Wang Jie , Huang Jing , Gao Qian , Wang Kaili , Wang Kun
Published: January 2023

Changes in species composition and diversity-productivity relationships during vegetation succession are of great significance for the restoration of degraded grasslands. However, the human intervention of grassland restoration mostly considers the single ecological (diversity) or production (biomass) function of grasslands, and does not fully consider the relationships between the two to improve the restoration effects. In this study, three vegetation types (desert grassland, sandy grassland and temperate savanna) were considered to simulate the three stages of restoration of degraded temperate savanna (early, middle and late restoration). Changes in species composition, diversity, and diversity-productivity relationships during the temperate savanna restoration were explored through spatiotemporal substitution. The results showed that with the progress of succession, xerophytes decreased significantly, mesophytes and hygrophytes increased, C4 plants decreased gradually, and C3 plants increased. The change of species richness was the main reason for community differences in the process of community succession, and species replacement mainly occurred between communities in the early and middle stages of succession. Species richness increased significantly from early to late stage of restoration. Latitude was significantly negatively correlated with species diversity. In the process of vegetation restoration, the impact of species richness, Pielou’s evenness, and β-diversity on biomass gradually developed positively and continued to increase, while the Simpson index showed the opposite effect. This study provides references for the restoration of temperate savanna and emphasizes the ecological and productive functions of the grassland should be taken into account in the restoration strategy.
Spatial characteristics of industrial economic location and its formation in Chongqing, China
Authors: Tang Zhonglin , Fu Min , Wang Yuting , Zhao Yihui
Published: January 2023

As the core carrier and organizational bodies of the regional industrial space, the study of the location of industrial enterprises and the formation of their economic location is related to the rational development of regional industries, the coordination of humans and the environment, and the effective allocation of resources. Taking Chongqing, one of the six old industrial bases in China, as an example, this study analyzed the spatial distribution characteristics, economic location characteristics, and formation laws of industrial enterprises based on the Points of interest data (POI), and investigation data. The results showed that industrial enterprises in Chongqing show obvious spatial clustering characteristics. About 93.50%, 60.34%, 96.67%, 97.57%, 73.57%, 64.83% of industrial enterprises were distributed within the spatial range of 10 Km from the motorways, national highways, provincial highways, county highways, main streams of rivers and central towns, and 93.48% of industrial enterprises were distributed at an altitude of 800 m or less. In order to further reveale the economic location characteristics of industrial enterprises in Chongqing, this study further quantified the spatial differentiation law of industrial economic location based on Geographically weighted regression (GWR). The results showed that factors such as the Distance to National Highways (DNH), Distance to County Highways (DCH), Distance to Central Towns (DCC), Distance to River systems (DR), and Population Density (POP) had significant positive impacts on the formation of economic locations of industrial enterprises, while the Distance to Motorway (DMW) exerted a certain negative influence, but the effectiveness sees strong spatial heterogeneity according to the type of industry and the actual regional industrial development, with factors such as transportation accessibility, environment, and labor force playing a moderating role.
Prenatal diagnosis of recurrent moderate skeletal dysplasias in lamin B receptors
Authors: Shen Xueping , Li Zhi , Pan Xuekui , Yao Juan , Shen Guosong , Zhang Su , Dong Minyue , Fan Lihong
Published: January 2023

The lamin B receptor (LBR) gene is located in chromosome 1q42.12 and encodes the lamin B receptor, an intracellular protein that binds to lamin B. LBR mutations are associated with a broad phenotypic spectrum ranging from non-lethal to lethal skeletal dysplasias. The typical phenotypes include the Pelger−Huet anomaly (PHA) and embryonic lethal Greenberg dysplasia (GRBGD). With the further study of this gene, other phenotypes have been found in different individuals. This retrospective study analyzed recurrent prenatal moderate skeletal dysplasias in Chinese fetuses. Nothing malformed was detected in the fetal karyotype and microarray, while the whole-exome sequencing identified a homozygous variant (NM_002296.4:c.1757G>A, NP_002287.2:p.Arg586His) in exon 14 of the LBR gene in both fetuses. Mutation analysis in the parents confirmed that the c.1757G>A variation is heterozygous by Sanger sequencing. Intensive analysis on bioinformatics and familial co-segregation suggest that the homozygous variation in the LBR gene is responsible for this recurrent prenatal moderate skeletal dysplasia. Moreover, moderate skeletal dysplasias differ from typical GRBGD phenotypes. Our findings are based on the DNA base test and the prenatal diagnosis of skeletal dysplasia, which can be helpful in proper phenotyping and contribute to a better understanding of the correlation between the phenotype and genotype.
Subjective sleep quality and fatigue assessment in Polish adult patients with primary immunodeficiencies: A pilot study
Authors: Grochowalska Kinga , Ziętkiewicz Marcin , Więsik-Szewczyk Ewa , Matyja-Bednarczyk Aleksandra , Napiórkowska-Baran Katarzyna , Nowicka-Sauer Katarzyna , Hajduk Adam , Sołdacki Dariusz , Zdrojewski Zbigniew
Published: January 2023

Introduction

Primary immunodeficiencies (PIDs) are clinically heterogeneous disorders caused by abnormalities in the immune system. However, PIDs are genetically determined and may occur at any age from early childhood to elderly age. Due to chronic patterns, the risk of malignancy and organ damage in patients with PIDs may affect any aspect of life, including sleep patterns. To our knowledge, the prevalence of insomnia and subjective sleep quality have not been investigated in patients with PIDs. Therefore, this pilot study was conducted to investigate sleep quality, the prevalence of sleep disturbances, and fatigue in adult patients with PIDs in Poland.

Methods

All participants were surveyed using the Athens Insomnia Scale, Pittsburgh Sleep Quality Index, Fatigue Severity Scale, and a questionnaire concerning general health and demographic data. We included 92 participants: 48 women (52.2%) and 44 men (47.8%).

Results

Participants’ mean age was 41.9 ± 13.9 years. The mean sleep duration was 7.0 ± 1.5 hours, and the mean sleep latency was 41.2 ± 53.1 minutes. Additionally, 44.6% of patients (n=41) had symptoms of insomnia and 44.6% (n=42) had poor sleep quality. Less than one-fourth (n=22; 23.9%) of the patients reported the use of sleeping pills; moreover, clinically significant fatigue was reported in 52.2% (n=48).

Discussion

Our investigation provides insight into the problem of sleep disturbances in patients with PIDs. Data have demonstrated that sleeping disorders with concomitant fatigue are common in patients with PID. Further studies are needed to determine the determinants of poor sleep quality in this specific group of patients.
CRISPR interference for sequence-specific regulation of fibroblast growth factor receptor A in Schistosoma mansoni
Authors: Du Xiaofeng , McManus Donald P. , French Juliet D. , Collinson Natasha , Sivakumaran Haran , MacGregor Skye R. , Fogarty Conor E. , Jones Malcolm K. , You Hong
Published: January 2023

Employing the flatworm parasite Schistosoma mansoni as a model, we report the first application of CRISPR interference (CRISPRi) in parasitic helminths for loss-of-function studies targeting the SmfgfrA gene which encodes the stem cell marker, fibroblast growth factor receptor A (FGFRA). SmFGFRA is essential for maintaining schistosome stem cells and critical in the schistosome-host interplay. The SmfgfrA gene was targeted in S. mansoni adult worms, eggs and schistosomula using a catalytically dead Cas9 (dCas9) fused to a transcriptional repressor KRAB. We showed that SmfgfrA repression resulted in considerable phenotypic differences in the modulated parasites compared with controls, including reduced levels of SmfgfrA transcription and decreased protein expression of SmFGFRA, a decline in EdU (thymidine analog 5-ethynyl-2’-deoxyuridine, which specifically stains schistosome stem cells) signal, and an increase in cell apoptosis. Notably, reduced SmfgfrA transcription was evident in miracidia hatched from SmfgfrA-repressed eggs, and resulted in a significant change in miracidial behavior, indicative of a durable repression effect caused by CRISPRi. Intravenous injection of mice with SmfgfrA-repressed eggs resulted in granulomas that were markedly reduced in size and a decline in the level of serum IgE, emphasizing the importance of SmFGFRA in regulating the host immune response induced during schistosome infection. Our findings show the feasibility of applying CRISPRi for effective, targeted transcriptional repression in schistosomes, and provide the basis for employing CRISPRi to selectively perturb gene expression in parasitic helminths on a genome-wide scale.
Intensification of “river in the sea” along the western Bay of Bengal Coast during two consecutive La Niña events of 2020 and 2021 based on SMAP satellite observations
Authors: Li Zhiyuan , Long Yu , Zhu Xiao-Hua , Papa Fabrice
Published: January 2023

A narrow strip of low salinity water that hugs the western Bay of Bengal (BoB) is known as a “river in the sea” (RIS). During the autumns of 2020 and 2021, Soil Moisture Active Passive (SMAP) identified an intensification of the RIS that was characterized by a continuous, uniform strip of low salinity water (<31 psu) that was 100 km wide extending along the western BoB coast to south of Sri Lanka. The analysis shows that the La Niña events in 2020–2021 were responsible for this intensification. During the La Niña events of 2020 and 2021, cyclonic circulation anomalies were generated along the BoB coast, causing a stronger Eastern Indian Coastal Current (EICC). Meanwhile, the northern BoB received increased river discharge during the summer monsoons of the two years. The stronger EICC and increased river discharge together led to anomalous freshwater transport that contributed to the intensification of the RIS. Notably, the RIS extended far southward during La Niña events of 2016 and 2017. However, the spatial structure of the RIS differed sharply between 2016–2017 and 2020–2021. The results indicate that pairs of eddies in the western BoB steered the freshwater offshore by several hundred kilometers. As a result, offshore extending tongue-shaped freshwater plumes were formed that inhibited the formation of an organized RIS structure along the western BoB coast during 2016–2017. This study highlights that the year-to-year variability of the RIS was significantly influenced by the La Niña events and modulated by mesoscale processes.
The promotion effect of uniaxial strain on hydrogen aggregation in tungsten
Authors: Ma Fang-Fei , Li Yu-Hao , Ren Qing-Yuan , Zhou Hong-Bo , Lu Guang-Hong
Published: January 2023

The aggregation behavior of hydrogen (H) isotopes after low-energy plasma irradiation is particularly important for tungsten (W) in future fusion devices. Here, using the first-principles calculation, we demonstrated an interesting strain effect that may drive the planar aggregation of interstitial H atoms in W. Although there are attractive interactions between H atoms, the spontaneous nucleation of these platelet-like H clusters in strain-free W appears impossible even at high H concentrations (up to 0.1 at.%) because of the extremely low configurational entropy. However, applied uniaxial strain significantly increased the binding energy of H platelets and enabled planar H clusters to form. These results suggest that uniaxial strain enhances the nucleation and growth of H platelets in W, regardless of whether it is a compressive or tensile strain. Moreover, the binding energy of one-layer H clusters was lower than that of parallel H platelets, implying that the formation of multi-layer H clusters in W and their stability is also promoted by uniaxial strain. Meanwhile, the presence of planar H clusters dramatically reduced the vacancy formation energy in W, which in turn provided an extra trapping site to accommodate excessive H atoms. These results provide an important reference for understanding the H evolution in W-PFMs.
Inhibition of IL-12 heterodimers impairs TLR9-mediated prevention of early mouse plasmacytoma cell growth
Authors: Mandour Mohamed F. , Soe Pyone Pyone , Castonguay Anne-Sophie , Van Snick Jacques , Coutelier Jean-Paul
Published: January 2023

Introduction

Natural prevention of cancer development depends on an efficient immunosurveillance that may be modulated by environmental factors, including infections. Innate lymphoid cytotoxic cells have been shown to play a major role in this immunosurveillance. Interleukin-12 (IL-12) has been suggested to be a key factor in the activation of innate cytotoxic cells after infection, leading to the enhancement of cancer immunosurveillance.

Methods

The aim of this work was to analyze in mouse experimental models by which mechanisms the interaction between infectious agent molecules and the early innate responses could enhance early inhibition of cancer growth and especially to assess the role of IL-12 by using novel antibodies specific for IL-12 heterodimers.

Results

Ligation of toll-like receptor (TLR)9 by CpG-protected mice against plasmacytoma TEPC.1033.C2 cell early growth. This protection mediated by innate cytolytic cells was strictly dependent on IL-12 and partly on gamma-interferon. Moreover, the protective effect of CpG stimulation, and to a lesser extent of TLR3 and TLR7/8, and the role of IL-12 in this protection were confirmed in a model of early mesothelioma AB1 cell growth.

Discussion

These results suggest that modulation of the mouse immune microenvironment by ligation of innate receptors deeply modifies the efficiency of cancer immunosurveillance through the secretion of IL-12, which may at least partly explain the inhibitory effect of previous infections on the prevalence of some cancers.
Isolation, identification, biological characteristics, and antifungal efficacy of sodium bicarbonate combined with natamycin on Aspergillus niger from Shengzhou nane (Prunus salicina var. taoxingli) fruit
Authors: Guo Tian-Rong , Zeng Qing , Yang Guo , Ye Si-Si , Chen Zi-Yi , Xie Shi-Ying , Wang Hai , Mo Yi-Wei
Published: January 2023

The fungi causing fruit rot were isolated from symptomatic Shengzhou nane (Prunus salicina var. taoxingli) fruit and were identified as Aspergillus niger by biological characteristics and molecular analysis of the internal transcribed spacer region (rDNA-ITS) and translation elongation factor-1α (TEF-1α) sequences. Optimal growth conditions for A. niger were 30°C, pH 5.0–6.0, and fructose and peptone as carbon and nitrogen sources. The effects of sodium bicarbonate (SBC), natamycin (NT), and combined treatments on A. niger inhibition were investigated. Treatment with 4.0 g/L sodium bicarbonate (SBC) + 5.0 mg/L natamycin (NT) inhibited mycelial growth and spore germination as completely as 12.0 mg/L SBC or 25.0 mg/L NT. SBC and NT treatments disrupted the structural integrity of cell and mitochondria membranes and decreased enzyme activities involved in the tricarboxylic acid (TCA) cycle, mitochondrial membrane potential (MMP), ATP production in mitochondria, and ergosterol content in the plasma membrane, thus leading to the inhibition of A. niger growth. Moreover, experimental results in vivo showed that the rot lesion diameter and decay rate of Shengzhou nane fruit treated with SBC and NT were significantly reduced compared with the control. The results suggest that the combination treatment of SBC and NT could be an alternative to synthetic fungicides for controlling postharvest Shengzhou nane decay caused by A. niger.
Comparative analysis of Penicillium genomes reveals the absence of a specific genetic basis for biocontrol in Penicillium rubens strain 212
Authors: Requena Elena , Alonso-Guirado Lola , Veloso Javier , Villarino María , Melgarejo Paloma , Espeso Eduardo Antonio , Larena Inmaculada
Published: January 2023

Penicillium rubens strain 212 (PO212) is a filamentous fungus belonging to the division Ascomycete. PO212 acts as an effective biocontrol agent against several pathogens in a variety of horticultural crops including Fusarium oxysporum f.sp. lycopersici, causing vascular wilt disease in tomato plants. We assembled draft genomes of two P. rubens strains, the biocontrol agent PO212 and the soil isolate S27, which lacks biocontrol activity. We also performed comparative analyses of the genomic sequence of PO212 with that of the other P. rubens and P. chrysogenum strains. This is the first Penicillium strain with biocontrol activity whose genome has been sequenced and compared. PO212 genome size is 2,982 Mb, which is currently organized into 65 scaffolds and a total of 10,164 predicted Open Reading Frames (ORFs). Sequencing confirmed that PO212 belongs to P. rubens clade. The comparative analysis of the PO212 genome with the genomes of other P. rubens and Penicillium chrysogenum strains available in databases showed strong conservation among genomes, but a correlation was not found between these genomic data and the biocontrol phenotype displayed by PO212. Finally, the comparative analysis between PO212 and S27 genomes showed high sequence conservation and a low number of variations mainly located in ORF regions. These differences found in coding regions between PO212 and S27 genomes can explain neither the biocontrol activity of PO212 nor the absence of such activity in S27, opening a possible avenue toward transcriptomic and epigenetic studies that may shed light on this mechanism for fighting plant diseases caused by fungal pathogens. The genome sequences described in this study provide a useful novel resource for future research into the biology, ecology, and evolution of biological control agents.
Methylmercury formation in biofilms of Geobacter sulfurreducens
Authors: Yunda Elena , Gutensohn Mareike , Ramstedt Madeleine , Björn Erik
Published: January 2023

Introduction

Mercury (Hg) is a major environmental pollutant that accumulates in biota predominantly in the form of methylmercury (MeHg). Surface-associated microbial communities (biofilms) represent an important source of MeHg in natural aquatic systems. In this work, we report MeHg formation in biofilms of the iron-reducing bacterium Geobacter sulfurreducens.

Methods

Biofilms were prepared in media with varied nutrient load for 3, 5, or 7 days, and their structural properties were characterized using confocal laser scanning microscopy, cryo-scanning electron microscopy and Fourier-transform infrared spectroscopy.

Results

Biofilms cultivated for 3 days with vitamins in the medium had the highest surface coverage, and they also contained abundant extracellular matrix. Using 3 and 7-days-old biofilms, we demonstrate that G. sulfurreducens biofilms prepared in media with various nutrient load produce MeHg, of which a significant portion is released to the surrounding medium. The Hg methylation rate constant determined in 6-h assays in a low-nutrient assay medium with 3-days-old biofilms was 3.9 ± 2.0 ∙ 10−14  L ∙ cell−1 ∙ h−1, which is three to five times lower than the rates found in assays with planktonic cultures of G. sulfurreducens in this and previous studies. The fraction of MeHg of total Hg within the biofilms was, however, remarkably high (close to 50%), and medium/biofilm partitioning of inorganic Hg (Hg(II)) indicated low accumulation of Hg(II) in biofilms.

Discussion

These findings suggest a high Hg(II) methylation capacity of G. sulfurreducens biofilms and that Hg(II) transfer to the biofilm is the rate-limiting step for MeHg formation in this systems.
Increasing resilience of material supply by decentral urban factories and secondary raw materials
Authors: Meyer K. , Görgens S. , Juraschek M. , Herrmann C.
Published: January 2023

Current production processes are frequently dependent on global supply chains for raw materials and prefabricated inputs. With rising political and global risks, these supply networks are threatened, which leads to a reduction of supply chain resilience. At the same time, urban areas are currently one of the main consumers of products and waste material generators. The raw material sourcing for this consumption commonly takes place in globally connected supply chains due to economy of scale effects. Therefore, cities are especially vulnerable to supply chain disruptions. A recent development which could reduce this vulnerability is the installation of urban factories among other urban production concepts, which can be symbiotically embedded into the urban metabolism to utilize the locally available (waste) materials. This, however, is hampered by the smaller production scale of decentralized urban production facilities, limited knowledge and challenges about the urban material flows and their characteristics. Against this background, we introduce a new factory type which is placed between the primary and secondary industrial sector: An urban secondary raw material factory which utilizes local waste material and other urban material flows for the extraction and refinement of secondary raw materials to supply production sites in its surrounding environment. To enable this small-to medium-scale factory type, the application of new production technologies plays a crucial role. Therefore, this paper proposes an approach for matching relevant potential waste streams to different technologies for waste-to-resource refinement. The applicability of the method for identification and evaluation of suitable technologies regarding their potential to be located in urban environments is demonstrated for plastic and metallic materials. Subsequently, key challenges and characteristics of the new factory type are summarized. With the introduction of this new factory type, the lack of scale effects in urban symbiotic networks is expected to be reduced. In conclusion, challenges such as the data-based management of symbiotic relationships among manufacturing companies are highlighted as still relevant in decentral value chains.
Analysis of hot trends in research on the association between vitamin D and cardiovascular disease
Authors: Luo Xuemei , Wu Feifeng , Wang Cheng , Wen Chuan
Published: January 2023

Objective

Vitamin D deficiency is the most common nutrient deficiency. Numerous studies suggest that vitamin D is an independent risk factor for cardiovascular disease. The objective is to visualize the research hotspots and evolution trends of the correlation between vitamin D and cardiovascular disease by using multivariate statistics and social network analysis techniques and to compare adult research with that of children in this field.

Methods

(Vitamin D [MeSH Major Topic]) AND (cardiovascular disease [MeSH Major Topic]) were retrieved from the PubMed database by time period. The bibliographic items co-occurrence matrix builder (BICOMB) was adopted to extract high-frequency subject terms and establish the core subject term co-occurrence matrix. With the Netdraw function of Ucinet 6.0 software, the social network of core subject terms was completed.

Results

Before 2010, there was a slow increase in the number of research papers covering all age groups in this field (157, 54, 84, and 211 papers were published in stages 1–4, respectively). From 2010 to 2020, there were 1,423 papers retrieved, showing a significantly increased research heat. The overall development trend of the research on the association between vitamin D and cardiovascular disease in children is similar to that in all age groups. From 2010 to 2020, 122 related papers were published (while before 2009, there were only 43 papers in all), presenting a good overall development trend. The social network analysis of core subject terms showed gradually increased correlations between research hotspots, from the early studies limited on the physiological function of vitamin D in cardiovascular diseases, to the role of vitamin D in the comorbidities of various cardiovascular diseases and its value as an intervention measure. Researches on the association between vitamin D and cardiovascular disease has a good overall development trend. Study of the mechanisms and the role of vitamin D in the common co-morbidities of cardiovascular disease and its therapeutic value will be the focus of future research.
Assessment of serum Vit A, D and zinc nutritional status and related dietary and exercise behaviors of children and adolescents in rural and urban area
Authors: Zou Yan , Huang Li-chun , Zhao Dong , He Meng-jie , Su Danting , Zhang Rong-hua
Published: January 2023

Vitamin A and vitamin D deficiency in children and adolescents has a negative impact on their growth and development. This study aimed to learn the nutritional status of vitamin A and D among rural children and adolescents and to explore related dietary and exercise behaviors. A total of 10 counties (cities, districts) from 90 counties (cities, districts) in Zhejiang Province were selected by the method of random cluster sampling. Children and adolescents were investigated and their food and nutrient intake were calculated. The concentration of serum vitamin A in urban area was 0.38 ng/ml, which was higher than that in rural area (p < 0.05); while the concentration of serum vitamin D in urban area was 21.25 mg/L, which was lower than that in rural area (p < 0.05). The concentration of serum zinc was 101 μg/dl in urban area and 107 μg/dl in rural area (p < 0.05). The intake of dietary fiber, calcium, vitamin A, vitamin B1, vitamin B2, and vitamin C was lower than the recommended value. In rural area, the intakes of cereals, tubers and beans, livestock, poultry and meat of children and adolescents were higher than the recommended values; while the intake of vegetables, fruits, eggs, milk, fish and shrimp, soybean and nuts was lower than the recommended value. The intake of edible oil and salt is higher than the recommended value. The time of medium and high intensity exercise time in rural area is more than that in urban area in the age group of 12–17 years, while the sitting time is less than that in urban area. Children and adolescents living in rural area should be guided to eat reasonably, and to choose foods with high nutrient density and with low oil, salt and sugar.
Survival, growth, and biogenic amine production of Enterococcus faecium FC12 in response to extracts and essential oils of Rubus fruticosus and Juniperus oxycedrus
Authors: Montanari Chiara , Barbieri Federica , Lorenzini Silvia , Gottardi Davide , Šimat Vida , Özogul Fatih , Gardini Fausto , Tabanelli Giulia
Published: January 2023

Introduction

Enterococci are lactic acid bacteria (LAB) usually found as food contaminants in fermented products such as cheeses and fermented sausages. Due to their antibiotic resistance, the presence of virulence factors, and the ability to produce biogenic amines (BAs), the determination of these bacteria is crucial to assure food quality and safety. BAs production and consequent accumulation in foods can cause toxicological effects on human health. Plant phenolic compounds are promising alternatives to chemical preservatives and reflect consumers' demand for “green” solutions. In this study, the antimicrobial effect of blackberry (Rubus fruticosus) leaves and prickly juniper (Juniperus oxycedrus) needles, both as phenolic extracts (PE) and essential oils (EO), were evaluated against Enterococcus faecium FC12, a known tyramine-producing strain.

Methods

The growth kinetics in the presence of sub-lethal concentrations of such plant derivatives were modeled (Gompertz equation) and BA production was monitored over time by HPLC. Moreover, flow cytometry (FCM) was used to study the effects of EOs and PEs on cell viability.

Results

The EOs showed a higher antimicrobial effect (especially R. fruticosus added at 0.75 mg/ml), determining an initial decrease of culturable cells followed by a recovery, even if with lower growth rates and final cell loads. Different rates of BA formation were observed, with tyramine concentrations ranging from 120 to 160 mg/l after 96 h of incubation, and 2-phenylethylamine was produced in lower amounts, usually after reaching the peak of tyramine. FCM confirmed the higher efficacy of R. fruticosus EO that induced cell membrane injury in 93% of the total population. However, complete recovery occurred in the following incubation, demonstrating transient damage.

Discussion

Although further research is required to better investigate this recovery and to assess the suitability of this approach in a real food system, the present study showed the potential antimicrobial activity of plant derivatives, especially R. fruticosus EO, against the tyramine-producing E. faecium FC12.
A novel hydrogel orthotopic injection model in moderately hypofractionated radiation therapy for prostate cancer: Adaptive degradation and durable imaging
Authors: Yu Hao , Wang Cheng , Wu Lingyun , Zhou Ziyang , Wang Yiqi , Li Wenxiang , Yuan Huili , Lu Zeyi , Yan Danfang , Chen Si , Wang Xu , Yan Senxiang
Published: January 2023

Purpose

Moderately hypofractionated radiotherapy (MHRT) holds an important position in prostate cancer management. Existing hydrogel spacers can protect the rectum from radiation damage, but need improvement. We explored the application of a novel hydrogel in MHRT with adaptive degradation and durable imaging functions.

Methods and materials

The hydrogels were irradiated with 6MV x-ray to detect the radio-resistance property. Male SD rats (n=45) underwent hydrogel injection between the prostate and rectum. CT was used for investigating the novel spacer’s degradation and imaging functions over three months. The hydrogel’s radiation-attenuation properties and biocompatibility were further assessed.

Results

Hydrogel weight and volume remained stable for six weeks post-injection. After MHRT ended, the hydrogel showed accelerated degradation characteristics and remained in the body for at most three months. CT values of hydrogels exceeded 300 Hounsfield units (HU) throughout treatment, significantly higher than in surrounding normal tissues. A significant dose drop behind the hydrogel was observed post-implantation. Biocompatibility tests of hydrogel found it safe enough for living organisms.

Conclusions

The novel hydrogel application was fully adaptable to prostate cancer MHRT modalities, largely stable during treatment, rapidly degraded after radiotherapy ended, and consistently maintained superior imaging performance and biocompatibility. This novel spacer will be an effective tool in the era of hypofractionated radiotherapy.
Research trend of circulating tumor DNA associated with breast cancer from 2012 to 2021: A bibliometric analysis
Authors: Ji Zeqi , Chen Lingzhi , Yang Qiuping , Tian Huiting , Wu Jinyao , Zheng Daitian , Cai Jiehui , Chen Yexi , Li Zhiyang
Published: January 2023

Background

Recently, ctDNA has become the focus for scientists with respect to personalized treatment, early screening, precise diagnosis, and prognosis of BC. This paper aims to use bibliometric analysis to investigate the research status and future trends in this field.

Methods

All the related literature in the field of ctDNA and breast cancer was gathered from the Web of Science Core Collection. Data analyses were performed with R package Bibliometrics, VOS viewer 1.6.18, and online analysis in WoS. IBM SPSS (version 26.0) was used for statistical analysis.

Results

A total of 739 publications, including 472 articles and 267 reviews, were retrieved. The overall number of articles published showed an upward trend. The United States has the largest number of published articles (266 papers) and citations (20,225 times). The most productive journal was Clinical Cancer Research. Cristofanilli M was the most prolific author, while Carlos C was the most cited one. The most frequent keywords excluding the search subject were “liquid biopsy”, “plasma”, “mutations”, “metastatic breast cancer”, “acquired resistance”.

Conclusion

This article explored the application value of ctDNA in breast cancer with bibliometric analysis, offering an overall and intuitive understanding of this topic and revealing the study trends in the past ten years.
Crosstalk between microwave ablation and ferroptosis: The next hot topic?
Authors: Yu Lu , Cheng Min , Liu Jie , Ye Xin , Wei Zhigang , Xu Jiamei , Xie Qi , Liang Jing
Published: January 2023

Microwave ablation has been one form of thermal ablation in treatments for many tumors, which can locally control unresectable tumors. Ferroptosis is iron-dependent cell death caused by the cumulative reactive oxygen species and lipid peroxidation products. Recently, increasing evidence has shown that ferroptosis might play a vital role in MWA-induced tumor suppression. In this article, we briefly illustrate the concept of ferroptosis, the related signal pathways and inducers, the basic principle of microwave ablation in killing tumors, and the key molecules released after microwave ablation. Then, we describe the cross-talking molecules between microwave ablation and ferroptosis, and discussed the potential mechanism of microwave ablation-induced ferroptosis. This review explores the therapeutic target of ferroptosis in enhancing the systemic antitumor effect after microwave ablation, providing theoretical support in combinational microwave ablation with pro-ferroptosis therapy.
Activity-related pain predicts pain and functional outcomes in people with knee osteoarthritis: A longitudinal study
Authors: Overton Mark , Swain Nicola , Falling Carrie , Gwynne-Jones David , Fillingim Roger , Mani Ramakrishnan
Published: January 2023

Knee Osteoarthritis (OA) is a prevalent musculoskeletal condition, commonly resulting in pain and disability. However, pain and disability in this population are poorly related with the degree of structural joint damage. Underlying pain mechanisms, including activity-related pain and sensitization assessed via Quantitative Sensory Testing (QST), may better predict pain and functional outcomes of those with knee OA. Therefore, the aim of this study was to explore whether activity-related pain and sensitization assessed via QST predict future pain, function, fatigue, physical performance and quality of life outcomes in those living in the community with knee OA. Eighty-six participants with knee OA were recruited in Dunedin, New Zealand. Those eligible to participate underwent baseline testing including QST as well as measures of activity-related pain including Movement-evoked Pain (MEP) and Sensitivity to Physical Activity (SPA). Outcome measures exploring pain, function, fatigue and quality of life outcomes were collected at baseline, and two follow-up periods (two and nine weeks). Univariable linear regression models were developed followed by multivariable linear regression models for each prognostic marker adjusting for age, gender, BMI, OA duration, baseline pain intensity and socioeconomic status. Activity-related measures of pain, including MEP and SPA, demonstrated predictive associations with pain and functional outcomes prospectively in those with knee OA. Therefore, those demonstrating activity-related pain are at future risk of greater pain, disability and reduced quality of life. Larger, externally validated longitudinal studies are required which include individuals with more severe knee OA.
Application of high-frequency ultrasound in the diagnosis of gastrointestinal magnet ingestion in children
Authors: Xin Yue , Jia Li Qun , Dong Ya Wei , Wang Yu , Hu Yan Xiu , Wang Xiao Man
Published: January 2023

Background

The incidence of magnet ingestion by children has recently increased in China. Magnet ingestion is associated with an extremely high risk of gastrointestinal damage because loops of bowel can become trapped and squeezed between multiple magnets in different locations. However, the lack of imaging sensitivity makes clinical decision-making difficult.

Objective

This study was conducted to investigate the performance of ultrasound in diagnosing gastrointestinal magnet ingestion in children.

Methods

From April 2017 to February 2021, all children with a history of magnet ingestion or a diagnosis of gastrointestinal magnet as shown by x-ray or ultrasound in our hospital were included as study candidates. Patients who were lost to follow-up or had known malformations of the gastrointestinal tract were excluded. Eligible patients were those with surgical or endoscopic confirmation of gastrointestinal magnet, those who passed the magnet out of the alimentary tract without assistance, and those with confirmed absence of the magnet on abdominal x-ray examination after 1 month of conservative treatment. All eligible patients' ultrasound and x-ray examination data were evaluated. The sensitivity, specificity, and area under the curve (AUC) of ultrasound was calculated for diagnosing magnet ingestion, locating the magnet (stomach, small intestine, or colon), and confirming the phenomenon of wall entrapment.

Results

Of 112 patients, 107 had a magnetic foreign body and 5 did not. Magnets were correctly detected by ultrasound in 97 patients, with an observed sensitivity of 90.65% and specificity of 100%. Satisfactory sensitivity was obtained for ultrasound localization of gastric magnets (96.30%) and small intestinal magnets (100.00%), but sensitivity for ultrasound localization of colonic magnets was relatively poor (73.33%). The discrimination of wall entrapment by ultrasound was good (AUC = 0.93), with an observed sensitivity and specificity of 92.00% and 93.62%, respectively.

Conclusions

Ultrasound can be used to locate gastrointestinal magnets (in the stomach, small intestine, or colon) with good clinical efficacy in identifying wall entrapment.
Parabrachial nucleus astrocytes regulate wakefulness and isoflurane anesthesia in mice
Authors: Liu Pei-Chang , Yao Wei , Chen Xing-Yu , Su Wei-Kun , Zheng Ze-Hong , Yan Xiong-Bin , Deng Ya-Ling , Shi Kai-Ge , Liu Xin , Gao Yu-Wei , Lin Tian-Tian , Zhu Yun-Xi , Lin Ying-Xuan , Zhu Zhong-Hua , Cai Ping , Zhang Liang-Cheng , Chen Li
Published: January 2023

Background: The parabrachial nucleus (PBN) is an important structure regulating the sleep–wake behavior and general anesthesia. Astrocytes in the central nervous system modulate neuronal activity and consequential behavior. However, the specific role of the parabrachial nucleus astrocytes in regulating the sleep-wake behavior and general anesthesia remains unclear.

Methods: We used chemogenetic approach to activate or inhibit the activity of PBN astrocytes by injecting AAV-GFAabc1d-hM3Dq-eGFP or AAV-GFAabc1d-hM4Di-eGFP into the PBN. We investigated the effects of intraperitoneal injection of CNO or vehicle on the amount of wakefulness, NREM sleep and REM sleep in sleep–wake behavior, and on the time of loss of righting reflex, time of recovery of righting reflex, sensitivity to isoflurane, electroencephalogram (EEG) power spectrum and burst suppression ratio (BSR) in isoflurane anesthesia.

Results: The activation of PBN astrocytes increased wakefulness amount for 4 h, while the inhibition of PBN astrocytes decreased total amount of wakefulness during the 3-hour post-injection period. Chemogenetic activation of PBN astrocytes decreased isoflurane sensitivity and shortened the emergence time from isoflurane-induced general anesthesia. Cortical EEG recordings revealed that PBN astrocyte activation decreased the EEG delta power and BSR during isoflurane anesthesia. Chemogenetic Inhibition of PBN astrocytes increased the EEG delta power and BSR during isoflurane anesthesia.

Conclusion: PBN astrocytes are a key neural substrate regulating wakefulness and emergence from isoflurane anesthesia.
Primary mapping of quantitative trait loci regulating multivariate horticultural phenotypes of watermelon (Citrullus lanatus L.)
Authors: Amanullah Sikandar , Li Shenglong , Osae Benjamin Agyei , Yang Tiantian , Abbas Farhat , Gao Meiling , Wang Xuezheng , Liu Hongyu , Gao Peng , Luan Feishi
Published: January 2023

Watermelon fruits exhibit a remarkable diversity of important horticultural phenotypes. In this study, we initiated a primary quantitative trait loci (QTL) mapping to identify the candidate regions controlling the ovary, fruit, and seed phenotypes. Whole genome sequencing (WGS) was carried out for two differentiated watermelon lines, and 350 Mb (96%) and 354 Mb (97%) of re-sequenced reads covered the reference de novo genome assembly, individually. A total of 45.53% non-synonymous single nucleotide polymorphism (nsSNPs) and 54.47% synonymous SNPs (sSNPs) were spotted, which produced 210 sets of novel SNP-based cleaved amplified polymorphism sequence (CAPS) markers by depicting 46.25% co-dominant polymorphism among parent lines and offspring. A biparental F2:3 mapping population comprised of 100 families was used for trait phenotyping and CAPS genotyping, respectively. The constructed genetic map spanned a total of 2,398.40 centimorgans (cM) in length and averaged 11.42 cM, with 95.99% genome collinearity. A total of 33 QTLs were identified at different genetic positions across the eight chromosomes of watermelon (Chr-01, Chr-02, Chr-04, Chr-05, Chr-06, Chr-07, Chr-10, and Chr-11); among them, eight QTLs of the ovary, sixteen QTLs of the fruit, and nine QTLs of the seed related phenotypes were classified with 5.32–25.99% phenotypic variance explained (PVE). However, twenty-four QTLs were identified as major-effect and nine QTLs were mapped as minor-effect QTLs across the flanking regions of CAPS markers. Some QTLs were exhibited as tightly localized across the nearby genetic regions and explained the pleiotropic effects of multigenic nature. The flanking QTL markers also depicted significant allele specific contributions and accountable genes were predicted for respective traits. Gene Ontology (GO) functional enrichment was categorized in molecular function (MF), cellular components (CC), and biological process (BP); however, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were classified into three main classes of metabolism, genetic information processing, and brite hierarchies. The principal component analysis (PCA) of multivariate phenotypes widely demonstrated the major variability, consistent with the identified QTL regions. In short, we assumed that our identified QTL regions provide valuable genetic insights regarding the watermelon phenotypes and fine genetic mapping could be used to confirm them.
Hypocrisy is the tribute that vice pays to virtue: Dynamics of perceived organization hypocrisy and job embeddedness in the hospitality industry
Authors: Zeb Faheem , Wang Qingjin , Shahjehan Asad
Published: January 2023

In the wake of the COVID-19 pandemic, hospitality institutions are striving for legitimacy, which leads them to organizational hypocrisy, generating perceptions of inducement breach, future anxiety, and ultimately reduced Job Embeddedness. This study has identified industry and environmental situation-specific constructs in a mutual relationship to fill a theoretical gap. An electronic survey of 2100 frontline employees was administered among which 842 completed surveys were retained for analysis. The validity of the measures and the absence of common method bias were established. SPSS PROCESS was used to compute the serial mediation effects. Contrary to existing knowledge, the results of this study indicate that organizational hypocrisy increases employee job embeddedness. Three reasons identified for this result are Asian culture sample, prevalence of COVID-19 pandemic, and the necessity of hypocrisy emphasized by scholars. The study also presents an underlying mechanism that makes this relationship negative through perceived inducement breach and future anxiety. This study focuses on HOW and IF organizational hypocrisy has detrimental effects, thus adding empirical evidence to otherwise exploratory literature. For hospitality industry, employees are an irreplaceable resource that provides competitive advantages; they need to align their values with that of their employees by word and actions or risk losing them.
A corpus-driven study of lexicalization models of English intransitive verbs
Authors: Wu Xiaofang , Xiao Kunxue , Wang Min , Yang Lifen
Published: January 2023

This bottom-up study applied a corpus-driven approach to extract the major lexicalization models of English intransitive verbs (EIVs) through an analysis of their meanings under the Ideal Motion Event Category inspired by Talmy. A 710-photo specialized multimodal corpus of EIVs was constructed for this purpose. Data analysis showed that the major lexicalization models of EIVs include [Motion + Patient], [Motion + Manner], [Motion + Path], [Motion + Result], [Motion + Location], and [Motion + Purpose]. In-depth analysis of these models identified three major possible reasons why EIVs cannot originally take direct objects: the incorporation of [Patient], the inheritance of the intransitive feature, and the internalization of the actions expressed by EIVs. By comparing Chinese learners’ most misused EIVs with their corresponding Chinese verbs, the current study provides empirical data to illustrate why transitive misusages of EIVs might occur among Chinese learners. The findings of this study will help English learners and users better apply EIVs.
Cyberdelics in context: On the prospects and challenges of mind-manifesting technologies
Authors: Hartogsohn Ido
Published: January 2023

The concept of cyberdelics emerged in the 1980s and 1990s as an umbrella term denoting the nexus connecting cybernetic (digital) technologies and psychedelic (mind manifesting) drugs. Cyberdelic technologies, in particular the then newly emerging field of virtual reality, were touted by psychedelic cultural icons including Timothy Leary and Terence McKenna as auguring a new era of digital mind-expansion where psychedelic experiences will be recreated online inside virtual worlds. Cyberdelic culture waned in the 2000s. However, recent years have seen the return of the cyberdelic imaginary, following on the heels of a psychedelic resurgence and a renewed interest in virtual reality technologies and their use in therapy. Cyberdelic advocates speak of the necessity of creating transformative technologies that steer humanity away from mindless consumerism and distractedness, and towards expanded states of awe, presence, and transcendence. Nevertheless, much like psychedelics, cyberdelic technologies are seen as running against the grain of current sociocultural arrangements and economic models which threaten to quell their transformative potential. Research on psychedelics within the humanities over the past decade has emphasized the role of cultural set and setting: the significance of the cultural embeddedness of these psychoactive agents and the dependence of their effects on surrounding sociocultural conditions. Building on the notion of information technologies as mind-manifesting technologies, this paper sets out to consider what psychedelics can teach us about cyberdelics: how the principles of set and setting and current discussions within the psychedelic humanities can inform our understanding of the resurgence of interest in cyberdelic media, its prospects, and challenges.