bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrodeposited Hydroxyapatite-Based Biocoatings: Recent Progress and Future Challenges
Authors: Safavi Mir Saman , Walsh Frank C. , Surmeneva Maria A. , Surmenev Roman A. , Khalil-Allafi Jafar
Published: January 2021

Hydroxyapatite has become an important coating material for bioimplants, following the introduction of synthetic HAp in the 1950s. The HAp coatings require controlled surface roughness/porosity, adequate corrosion resistance and need to show favorable tribological behavior. The deposition rate must be sufficiently fast and the coating technique needs to be applied at different scales on substrates having a diverse structure, composition, size, and shape. A detailed overview of dry and wet coating methods is given. The benefits of electrodeposition include controlled thickness and morphology, ability to coat a wide range of component size/shape and ease of industrial processing. Pulsed current and potential techniques have provided denser and more uniform coatings on different metallic materials/implants. The mechanism of HAp electrodeposition is considered and the effect of operational variables on deposit properties is highlighted. The most recent progress in the field is critically reviewed. Developments in mineral substituted and included particle, composite HAp coatings, including those reinforced by metallic, ceramic and polymeric particles; carbon nanotubes, modified graphenes, chitosan, and heparin, are considered in detail. Technical challenges which deserve further research are identified and a forward look in the field of the electrodeposited HAp coatings is taken.
Special Issue: Plasma Electrolytic Oxidation (PEO) Coatings
Authors: Mohedano Marta , Mingo Beatriz
Published: January 2021

The demand of modern technological society for light structural materials (Al, Ti, Mg) emphasizes a combination of good corrosion resistance with wear properties and functionalized surfaces [...]
Factors Associated with Meeting the Psychosocial Needs of Cancer Survivors in Nova Scotia, Canada
Authors: Chahine Soleil , Walsh Gordon , Urquhart Robin
Published: January 2021

Purpose: The purpose of this study is to describe the psychosocial needs of cancer survivors and examine whether sociodemographic factors and health care providers accessed are associated with needs being met. Methods: All Nova Scotia survivors meeting specific inclusion and exclusion criteria are identified from the Nova Scotia Cancer Registry and sent an 83-item survey to assess psychosocial concerns and whether and how their needs were met. Descriptive statistics (frequencies, percentages) and Chi-square analyses are used to examine associations between sociodemographic and provider factors and outcomes. Results: Anxiety and fear of recurrence, depression, and changes in sexual intimacy are major areas of concern for survivors. Various sociodemographic factors, such as immigration status, education, employment, and internet use, are associated with reported psychosocial health and having one’s needs met. Having both a specialist and primary care provider in charge of follow-up care is associated with a significantly (p < 0.05) higher degree of psychosocial and informational needs met compared to only one physician or no follow-up physician in charge. Accessing a patient navigator also is significantly associated with a higher degree of needs met. Conclusions: Our study identifies the most prevalent psychosocial needs of cancer survivors and the factors associated with having a higher degree of needs met, including certain sociodemographic factors, follow-up care by both a primary care practitioner and specialist, and accessing a patient navigator.
Identifying Best Implementation Practices for Smoking Cessation in Complex Cancer Settings
Authors: Giannopoulos Eleni , Papadakos Janet , Cameron Erin , Brual Janette , Truscott Rebecca , Evans William K. , Giuliani Meredith Elana
Published: January 2021

Background: In response to evidence about the health benefits of smoking cessation at time of cancer diagnosis, Ontario Health (Cancer Care Ontario) (OH-CCO) instructed Regional Cancer Centres (RCC) to implement smoking cessation interventions (SCI). RCCs were given flexibility to implement SCIs according to their context but were required to screen new patients for tobacco status, advise patients about the importance of quitting, and refer patients to cessation supports. The purpose of this evaluation was to identify practices that influenced successful implementation across RCCs. Methods: A realist evaluation approach was employed. Realist evaluations examine how underlying processes of an intervention (mechanisms) in specific settings (contexts) interact to produce results (outcomes). A realist evaluation may thus help to generate an understanding of what may or may not work across contexts. Results: The RCCs with the highest Tobacco Screening Rates used a centralized system. Regarding the process for advising and referring, three RCCs offered robust smoking cessation training, resulting in advice and referral rates between 80% and 100%. Five RCCs surpassed the target for Accepted Referral Rates; acceptance rates for internal referral were highest overall. Conclusion: Findings highlight factors that may influence successful SCI implementation.
Radiological Findings in Multidetector Computed Tomography (MDCT) of Hereditary and Sporadic Pulmonary Veno-Occlusive Disease: Certainties and Uncertainties
Authors: Pérez Núñez Marta , Alonso Charterina Sergio , Pérez-Olivares Carmen , Revilla Ostolaza Yolanda , Morales Ruiz Rafael , Enguita Valls Ana Belén , Tenorio Jair Antonio , Gallego Zazo Natalia , De Pablo Gafas Alicia , Lapunzina Pablo , Rodríguez Chaverri Adriana , Escribano Subías Pilar
Published: January 2021

Pulmonary veno-occlusive disease (PVOD) is a very infrequent form of pulmonary arterial hypertension with an aggressive clinical course, poor response to specific vasodilator treatment, and low survival. Confirming a definitive diagnosis is essential to guide treatment and assess lung transplantation. However, in the absence of histological or genetic confirmation, the diagnosis is complex, requiring a clinical suspicion. Multidetector computed tomography (MDCT) is an essential part of the non-invasive diagnostic tools of PVOD. We retrospectively reviewed the MDCT findings from a consecutive series of 25 patients diagnosed with PVOD, 9 with the sporadic form and 16 with the hereditary form of the disease. The presence and extent of typical findings of the diagnostic triad were assessed in all patients (ground glass parenchymal involvement, septal lines, and lymphadenopathy). In our series, 92% of patients showed at least two of the radiological findings described as typical of the disease. All patients presented at least one typical radiological characteristic. The incidence of radiological findings considered typical is very high, however was not associated with greater hemodynamic severity nor to the development of acute lung edema. No significant differences were found between the two groups. A poorly expressive MDCT does not exclude the disease.
The Prognostic Relevance of Poly (ADP-Ribose) Polymerase Expression in Ovarian Cancer Tissue of Wild Type and BRCA-Mutation Carrier Patients
Authors: Molnár Szabolcs , Vida Beáta , Beke Lívia , Méhes Gábor , Póka Róbert
Published: January 2021

(1) Background: The mechanism of platinum resistance in ovarian cancer is not fully clarified, but the properly functioning DNA repair mechanism can counteract the effect of conventional anticancer treatment. The objective of our study was to evaluate the expression of an important DNA repair enzyme, the Poly (ADP-Ribose) Polymerase (PARP) expression in epithelial ovarian cancer (EOC) tissues depending on BRCA status and to assess its relationship with platinum resistance. (2) Methods: Immunostaining to highlight PARP protein expression was performed using a rabbit polyclonal anti-PARP antibody. The intensity and distribution of immunostaining were assessed by light. Somatic BRCA1 or BRCA2 mutation carriers were identified with bidirectional sequencing of DNA from archived tumor tissue, if the test could not be performed due to technical reasons from tumor cells, the sequencing was done from peripheral blood cells to identify germline mutation carriers. The median progression-free survival (PFS) was generated for each semiquantitative group of PARP expression among chemotherapy-naive cases at the time of PARP immunohistochemistry. (3) Results: In the overall population, negative PARP immunohistochemistry predicted significant PFS (20.1 vs. 11.9 months, p = 0.001) and OS (49 vs. 114 months, p = 0.014) benefit. Genotype-stratified subgroup analysis in BRCA-negative cases confirmed the role of PARP positivity indicating an unfavorable prognosis in the entire population (relapsed 73.91% vs. 92%; OR: 4.06; p = 0.04). In the cases of the subgroup carrying the BRCA mutation, the presence of PARP expression was not associated with less favorable relapse rates, but with marginal significance for overall survival predicted a lower chance of survival (OS more than 32 months 72.73% vs. 35%; OR: 0.2; p = 0.05). (4) Conclusion: The BRCA wild type patients with strong expression of PARP enzymes before the first set of chemotherapy have a poor prognosis.
Information-Theoretic Generalization Bounds for Meta-Learning and Applications
Authors: Jose Sharu Theresa , Simeone Osvaldo
Published: January 2021

Meta-learning, or “learning to learn”, refers to techniques that infer an inductive bias from data corresponding to multiple related tasks with the goal of improving the sample efficiency for new, previously unobserved, tasks. A key performance measure for meta-learning is the meta-generalization gap, that is, the difference between the average loss measured on the meta-training data and on a new, randomly selected task. This paper presents novel information-theoretic upper bounds on the meta-generalization gap. Two broad classes of meta-learning algorithms are considered that use either separate within-task training and test sets, like model agnostic meta-learning (MAML), or joint within-task training and test sets, like reptile. Extending the existing work for conventional learning, an upper bound on the meta-generalization gap is derived for the former class that depends on the mutual information (MI) between the output of the meta-learning algorithm and its input meta-training data. For the latter, the derived bound includes an additional MI between the output of the per-task learning procedure and corresponding data set to capture within-task uncertainty. Tighter bounds are then developed for the two classes via novel individual task MI (ITMI) bounds. Applications of the derived bounds are finally discussed, including a broad class of noisy iterative algorithms for meta-learning.
Bi-Objective Workflow Scheduling on Heterogeneous Computing Systems Using a Memetic Algorithm
Authors: Zhang Yujian , Tong Fei , Li Chuanyou , Xu Yuwei
Published: January 2021

Due to the high power bills and the negative environmental impacts, workflow scheduling with energy consciousness has been an emerging need for modern heterogeneous computing systems. A number of approaches have been developed to find suboptimal schedules through heuristics by means of slack reclamation or trade-off functions. In this article, a memetic algorithm for energy-efficient workflow scheduling is proposed for a quality-guaranteed solution with high runtime efficiency. The basic idea is to retain the advantages of population-based, heuristic-based, and local search methods while avoiding their drawbacks. Specifically, the proposed algorithm incorporates an improved non-dominated sorting genetic algorithm (NSGA-II) to explore potential task priorities and allocates tasks to processors by an earliest finish time (EFT)-based heuristic to provide a time-efficient candidate. Then, a local search method integrated with a pruning technique is launched with a low possibility, to exploit the feasible region indicated by the candidate schedule. Experimental results on workflows from both randomly-generated and real-world applications suggest that the proposed algorithm achieves bi-objective optimization, improving makespan, and energy saving by 4.9% and 24.3%, respectively. Meanwhile, it has a low time complexity compared to the similar work HECS.
Measurements and Computations of Internal Temperatures of the IGBT and the Diode Situated in the Common Case
Authors: Górecki Paweł , Górecki Krzysztof
Published: January 2021

This article proposes effective methods of measurements and computations of internal temperature of the dies of the Insulted Gate Bipolar Transistor (IGBT) and the diode mounted in the common case. The nonlinear compact thermal model of the considered device is proposed. This model takes into account both self-heating phenomena in both dies and mutual thermal couplings between them. In the proposed model, the influence of the device internal temperature on self and transfer thermal resistances is taken into account. Methods of measurements of each self and transfer transient thermal impedances occurring in this model are described and factors influencing the measurement error of these methods are analysed. Some results illustrating thermal properties of the investigated devices including the IGBT and the antiparallel diode in the common case are shown and discussed. Computations illustrating the usefulness of the proposed compact thermal model are presented and compared to the results of measurements. It is proved that differences between internal temperature of both dies included in the TO-247 case can exceed even 15 K.
Improving the Prediction Quality in Memory-Based Collaborative Filtering Using Categorical Features
Authors: Chen Lei , Yuan Yuyu , Yang Jincui , Zahir Ahmed
Published: January 2021

Despite years of evolution of recommender systems, improving prediction accuracy remains one of the core problems among researchers and industry. It is common to use side information to bolster the accuracy of recommender systems. In this work, we focus on using item categories, specifically movie genres, to improve the prediction accuracy as well as coverage, precision, and recall. We derive the user’s taste for an item using the ratings expressed. Similarly, using the collective ratings given to an item, we identify how much each item belongs to a certain genre. These two vectors are then combined to get a user-item-weight matrix. In contrast to the similarity-based weight matrix in memory-based collaborative filtering, we use user-item-weight to make predictions. The user-item-weights can be used to explain to users why certain items have been recommended. We evaluate our proposed method using three real-world datasets. The proposed model performs significantly better than the baseline methods. In addition, we use the user-item-weight matrix to alleviate the sparsity problem associated with correlation-based similarity. In addition to that, the proposed model has a better computational complexity for making predictions than the k-nearest neighbor (kNN) method.
Analysis of Cavity PD Characteristics’ Sensitivity to Changes in the Supply Voltage Frequency
Authors: Venge Tapiwa , Nyamupangedengu Cuthbert
Published: January 2021

The supply voltage frequency effect on partial discharge (PD) phenomena has continued to draw research interest. Although most high voltage equipment operates at power frequency (50/60 Hz), testing is often done at different frequencies for various reasons. Despite some agreements and inconsistencies for the research findings of PD activity’s frequency dependence, there has been consensus on the recognition of the discharge mechanism parameters that influence how the supply voltage frequency affects PD activity. These parameters include statistical time lag, discharge area surface conductivity, and the residual charge decay. In this paper, a 3-capacitor model (ABC) is used to simulate how the changes in the discharge mechanism parameters influence PD characteristics as a function of the supply voltage frequency. The findings are that the phase-resolved partial discharge pattern (PRPDP) and PD repetition rate (PDRR) characteristics are more sensitive to variations in the probability of the seed electron availability at higher frequencies of the supply voltage. The opposite trend is observed for the cavity surface resistance. At lower resistance of cavity surface, the PRPDP and PDRR characteristics are more sensitive to changes in the supply voltage frequency than at higher resistances. The paper also confirms that incorporating equivalent resistances in the ABC model makes it more authentic than the model comprising of capacitors only.
Multi-Fidelity Surrogate Models for Predicting Averaged Heat Transfer Coefficients on Endwall of Turbine Blades
Authors: Choi Woosung , Radhakrishnan Kanmaniraja , Kim Nam-Ho , Park Jun Su
Published: January 2021

This paper proposes a multi-fidelity surrogate (MFS) model for predicting the heat transfer coefficient (HTC) on the turbine blades. First, the low-fidelity (LF) and high-fidelity (HF) surrogates were built using LF-data from numerical simulation and HF-data from an experiment. To evaluate the prediction by these two surrogates, the averaged HTC distribution on the endwall of the gas turbine blade predicted by these two surrogates was compared for input variables as Reynolds number (Re) and boundary layer (BL) thickness. This shows that the prediction by LF surrogate is saturated with an increase in Re, while has monotonic behavior with an increase in BL thickness, which is contrary in general. The prediction by HF surrogate is linear with Re and is increased with BL thickness up to 30 mm and then decreased after 30 mm. Following this, a one-dimensional projection of the two-dimensional HTC distribution was presented to show the prediction tendency of the surrogates by varying the Re and fixing the BL thickness, and vice versa. Second, the MFS was built by combining the LF and HF data. The HTC distribution by the MFS model for the same input variables was shown with the HF data points. It is observed that the prediction by MFS is agreed well with the high-fidelity data. The MFS’s one-dimensional projection of the two-dimensional HTC distribution was compared with the LF surrogate prediction by varying the Re and fixing the BL thickness, and vice versa. This shows that the MFS model has more variations due to the included LF data. It is worth to mention that the averaged HTC distribution with an increase in boundary layer thickness predicted by the MFS is agreed well with the LF and HF data in the available dataset and has a large confidence interval between 30 and 50 mm due to the unavailable data in the specified range. To check the MFS accuracy, the root-mean-square error (RMSE) and error rate were evaluated to compare with the experimental uncertainty for a wide range of high-fidelity data. The present study shows that MFS would be expected to be an effective model for saving computing time and experimental costs.
A New MPPT Algorithm for Photovoltaic Power Generation under Uniform and Partial Shading Conditions
Authors: Windarko Novie Ayub , Nizar Habibi Muhammad , Sumantri Bambang , Prasetyono Eka , Efendi Moh. Zaenal , Taufik
Published: January 2021

During its operation, a photovoltaic system may encounter many practical issues such as receiving uniform or non-uniform irradiance caused mainly by partial shading. Under uniform irradiance a photovoltaic panel has a single maximum power point. Conversely under non-uniform irradiance, a photovoltaic panel has several local maximum power points and a single global maximum power point. To maximize energy production, a maximum power point tracker algorithm is commonly implemented to achieve the maximum power operating point of the photovoltaic panel. However, the performance of the algorithm will depend on operating conditions such as variation in irradiance. Presently, most of existing maximum power point tracker algorithms work only in a single condition: either uniform or non-uniform irradiance. This paper proposes a new maximum power point tracker algorithm for photovoltaic power generation that is designed to work under uniform and partial shading irradiance conditions. Additionally, the proposed maximum power point tracker algorithm aims to provide: (1) a simple math algorithm to reduce computational load, (2) fast tracking by evaluating progress for every single executed duty cycle, (3) without random steps to prevent jumping duty cycle, and (4) smooth variable steps to increase accuracy. The performances of the proposed algorithm are evaluated by three conditions of uniform and partial shading irradiance where a targeted maximum power point is located: (1) far from, (2) near, and (3) laid between initial positions of particles. The simulation shows that the proposed algorithm successfully tracks the maximum power point by resulting in similar power values in those three conditions. The proposed algorithm could handle the partial shading condition by avoiding the local maxima power point and finding the global maxima power point. Comparisons of the proposed algorithm and other well-known algorithms such as differential evolution, firefly, particle swarm optimization, and grey wolf optimization are provided to show the superiority of the proposed algorithm. The results show the proposed algorithm has better performance by providing faster tracking, faster settling time, higher accuracy, minimum oscillation and jumping duty cycle, and higher energy harvesting.
Al-ZSM-5 Nanocrystal Catalysts Grown from Silicalite-1 Seeds for Methane Conversion
Authors: Kim Hyun Su , Kang Su Kyung , Zhang Haoxiang , Tikue Elsa Tsegay , Lee Jin Hyung , Lee Pyung Soo
Published: January 2021

This study evaluated Al-ZSM-5 nanocrystals grown from silicalite-1 seed crystals as catalysts for the methane dehydroaromatization (MDA) reaction. Silicalite-1 seed crystals sized between 30 and 40 nm were used to grow Al-ZSM-5 under various synthesis conditions. The size of Al-ZSM-5 was significantly affected by the Si/Al ratio (SAR), synthesis time, and silica nutrients/seed crystal ratio (NSR). Larger crystals were obtained with an increased SAR in the synthesis sols. Gradual growth of Al-ZSM-5 occurred with synthesis time, although the growth in crystal size ceased at 5 h of synthesis at 120 °C, indicating the rapid growth of Al-ZSM-5 aided by the silicalite-1 seeds. Precise tuning of Al-ZSM-5 size was possible by changing the nutrient/silicalite-1 seed ratio; a higher NSR led to larger crystals. Two representative Al-ZSM-5 crystals with SARs of 35 and 140 were prepared for catalyst testing, and the crystal sizes were tailored to <100 nm by controlling NSR. The MDA reaction was conducted in the presence of the prepared Al-ZSM-5. The catalyst size exhibited distinct differences in catalyst stability, while the SAR of catalysts did not produce noticeable changes in the catalyst stability of the Al-ZSM-5 crystals and commercial zeolites in this reaction system.
Oxidation Stability of Natural Ester Modified by Means of Fullerene Nanoparticles
Authors: Szcześniak Dominika , Przybylek Piotr
Published: January 2021

Increasing environmental demands influence the requirements for devices and materials used in the power industry. One example is a power transformer and an electro-insulating liquid used in it. In order to meet these requirements, electro-insulating liquids should be characterized by, inter alia, high biodegradability and good fire properties. One of such liquids is natural ester. However, its oxidation stability is low in comparison to mineral oil and demands improvement, which can be achieved by the addition of an antioxidant. The authors of this work used fullerene nanoparticles for that purpose. Pure natural ester samples were prepared, and samples with two concentrations of fullerene, 250 mg/L and 500 mg/L in natural ester. All these samples were aged in a thermal oxidation process. Thereafter, the aging properties of all the samples were compared to assess the oxidation stability of modified liquids. Moreover, the electrical properties of prepared insulating liquids were investigated to assess if fullerene deteriorates these properties after aging process. Based on the obtained results, it was proved that the aging process slowed down in the case of both fullerene concentrations in ester. The acid number of natural ester modified using fullerene was lower than in the case of pure liquid.
Phytosociological Analysis of Natural and Artificial Pine Forests of the Class Vaccinio-Piceetea Br.-Bl. in Br.-Bl. et al. 1939 in the Sudetes and Their Foreland (Bohemian Massif, Central Europe)
Authors: Reczyńska Kamila , Pech Paweł , Świerkosz Krzysztof
Published: January 2021

Research Highlights: Differentiation of Scots pine forests of the class Vaccinio-Piceetea in Poland has been the subject of numerous studies, including revisions. Despite that, the area of southwestern Poland was hitherto practically unexplored in this respect. Background and Objectives: The aim of this work was therefore (i) to present the diversity of the pine forests in the Sudetes and their foreland; (ii) to compare the ecology of studied communities. Materials and Methods: We analyzed 175 phytosociological relevés collected between 1991 and 2020 in natural and anthropogenic pine stands. To identify vegetation types, we used the modified TWINSPAN algorithm; principal coordinate analysis, distance-based redundancy analysis and permutational tests were applied to identify the variation explained and the main environmental gradients shaping the studied plant communities. Results: Five associations were distinguished: thermophilous Asplenio cuneifolii-Pinetum sylvestris Pišta ex Husová in Husová et al. 2002, which develops on shallow soils over ultrabasic substrates, Hieracio pallidi-Pinetum sylvestris Stöcker 1965, which prefers outcrops of acidic rocks; Betulo carpaticae-Pinetum sylvestris Mikyška 1970, which is relict in origin and occurs on the upper Cretaceous sandstones, the peatland pine–birch forests of the Vaccinio uliginosi-Betuletum pubescentis Libbert 1933 and the Vaccinio myrtilli-Pinetum sylvestris Juraszek 1928. Moreover, community Brachypodium sylvaticum-Pinus sylvestris with the occurrence of many thermophilous and basiphilous species was also found on limestone substratum. The analysis of the species composition of pine plantations established on deciduous and mixed forests habitats revealed that these anthropogenic communities were marked by a random combination of species in which a certain group of common forest generalists participated. The distinguished communities differed clearly among each other also in habitat characteristics. Particularly important for their differentiation were soil reaction and nutrients, supported by differences in moisture, temperature and light availability. Apart from the edaphic factors, altitude and the bedrock type proved to be equally important. Conclusions: Our study provides new remarks to the typology and synecology of pine forest communities in SW Poland.
Effect of Veneer Initial Moisture Content on the Performance of Polyethylene Film Reinforced Decorative Veneer
Authors: Fang Lu , Zeng Jian , Zhang Xinhao , Wang Dan
Published: January 2021

The flexible modification of decorative veneer by plastic film is an effective method to broaden its applications. In order to understand the effect of initial veneer moisture content on the performance of plastic film reinforced decorative veneer, Fraxinus mandshurica veneers with different initial moisture contents were composited with polyethylene film. The overlaying performance of the decorative veneer and its interface morphology with MDF substrate were evaluated. The results showed that when the initial moisture content increased from 10% to 50%, the cavity proportion of PE film reinforced decorative veneer increased from 7.23% to 18.48%, while all of the minimum steel rod diameters remained at 7.6 mm. When the initial moisture content fluctuated between 10% and 20%, the optimum surface bonding strength (1 MPa) and immersion peel strength (0 mm) of the decorative veneer could be obtained. The strength of the decorative veneer significantly decreased when the initial moisture content was more than 20%, which was caused by the cavities and the poor interfacial bonding. The veneer surface was easy to crack under low moisture content conditions. Based on the overall performance and the cost of decorative wood-based panels, initial veneer moisture content was suggested to be 15% to 20%.
Potential Differences and Methods of Determining Gypsy Moth Female Flight Capabilities: Implications for the Establishment and Spread in Novel Habitats
Authors: Srivastava Vivek , Keena Melody A. , Maennicke Galen E. , Hamelin Richard C. , Griess Verena C.
Published: January 2021

The introduction of the Asian gypsy moth into novel environments continues with frequent interceptions in North America. There is a concern that these subspecies will pose a greater threat to the forests and urban environments of North America than the established gypsy moths (Lymantria dispardispar L.), due to their greater capacity for female flight. Asian gypsy moth populations vary in many key traits, including female flight capabilities. The potential impacts of female flight, in combination with the other key traits, on the ecology and spread of this insect are first discussed in this communication. This also provides the first review of most of the current literature on the variations in flight capability and flight distance of gypsy moth populations, as well as variation in other traits of concern and the potential methods of identification, with special attention paid to the Asian subspecies Lymantria dispar japonica Motschulsky and Lymantria dispar asiatica Vinkovskij. There are currently good tools for identifying the general origin of introduced gypsy moth populations, but these do not provide enough information to effectively manage introductions. Gypsy moth key traits differ among populations, even within each subspecies of the gypsy moth, so introduction of gypsy moths from other world areas into locations where the gypsy moth is already present could result in unwanted changes in gypsy moth biology. It also appears that the introduction of flight-capable females could enhance a population’s dispersal capability and require modifications to management protocols used for flightless females. Therefore, rapid tools to assess key traits in introduced populations are needed to adequately plan for, or deal with, new introductions into novel habitats.
Soil Biodiversity as Affected by Different Thinning Intensities in a Pinus laricio Stand of Calabrian Apennine, South Italy
Authors: Muscolo Adele , Settineri Giovanna , Romeo Federico , Mallamaci Carmelo
Published: January 2021

Forest soil biodiversity, which drives natural ecosystem multifunctionality, can be altered by incorrect forestry management practices. Pinus laricio is the most representative and widespread conifer species in Calabria, South Italy, and appropriate management is needed to maintain Pinus laricio forest for its great economic and natural value. In Europe, thinning is considered the most effective silvicultural treatment to maintain/increase the ecological value of coniferous stands. In this study, moderate thinning (MT), intense thinning (HT), and clear cut (CC) treatments were used to manage Pinus laricio stands with the aim of identifying the thinning intensity that is less detrimental to soil biodiversity. The effects of the different thinning intensities were evaluated, in two contrasting seasons (summer and winter), on the abundance, and diversity of arthropods, fungi, and bacteria colonies as well as on selected soil properties (organic matter, humification index, bulk density, pH) related to soil habitability. Results evidenced that the abundance, species richness, and diversity of arthropods, as well as fungi, bacteria colonies, and soil properties, changed with the treatments and seasons. Under HT, the greatest biodiversity and the highest amounts of arthropods, fungi, and bacteria were found in both seasons. This study finds evidence for Connell’s intermediate disturbance hypothesis, highlighting that the greatest organic carbon content and humification index, as well as the lowest bulk density, found in HT reduced the likelihood of competitive exclusion between occurring species, thereby promoting high species richness and diversity. This study gives insights into ecological relationships between understory composition related to tree species abundance and soil community.
Analysis of Intact Glycosidic Aroma Precursors in Grapes by High-Performance Liquid Chromatography with a Diode Array Detector
Authors: Cebrián-Tarancón Cristina , Oliva José , Cámara Miguel Ángel , Alonso Gonzalo L. , Salinas M. Rosario
Published: January 2021

Nowadays, the techniques for the analysis of glycosidic precursors in grapes involve changes in the glycoside structure or it is necessary the use of very expensive analytical techniques. In this study, we describe for the first time an approach to analyse intact glycosidic aroma precursors in grapes by high-performance liquid chromatography with a diode array detector (HPLC-DAD), a simple and cheap analytical technique that could be used in wineries. Briefly, the skin of Muscat of Alexandria grapes was extracted using a microwave and purified using solid-phase extraction combining Oasis MCX and LiChrolut EN cartridges. In total, 20 compounds were selected by HPLC-DAD at 195 nm and taking as a reference the spectrum of phenyl β-D-glucopyranoside, whose DAD spectrum showed a first shoulder from 190 to 230 nm and a second around 200–360 nm. After that, these glycosidic compounds were identified by High-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (HPLC-qTOF-MS). Disaccharides hexose pentose were the most abundant group observed with respect to the sugars and monoterpendiols the main aglycones found.
Innovative Characterization Based on Stress Relaxation and Creep to Reveal the Tenderizing Effect of Ultrasound on Wooden Breast
Authors: Li Zhen , Yang Zongyun , Zhang Yulong , Lu Tong , Zhang Xiaoqian , Qi Yue , Wang Peng , Xu Xinglian
Published: January 2021

In order to explore a new strategy to characterize the texture of raw meat, based on the ultrasonic tenderized wooden breast (WB), this study proposed stress relaxation and creep to determine the rheological properties. Results showed that hardness was significantly decreased from 3625.61 g to 2643.64 g, and elasticity increased, after 600 W ultrasound treatment at 20 kHz for 20 min (on-time 2 s and off-time 3 s) at 4 °C. In addition, based on the transformation of creep data, a new indicator, slope ε′(t), was innovatively used to simulate a sensory feedback of hardness from the touch sensation, proving WB became tender at 600 W treatment due to the feedback speed to external force. These above results were confirmed by the reduced shear force, increased myofibril fragmentation index (MFI), decreased particle size, and increased myofibrillar protein degradation. Histology analysis and collagen suggested the tenderizing results was caused by muscle fiber rather than connective tissue. Overall, stress relaxation and creep had a potential to predict meat texture characteristics and 600 W ultrasound treatment was an effective strategy to reduce economic losses of WB.
Native Species Facing Climate Changes: Response of Calafate Berries to Low Temperature and UV Radiation
Authors: Romero-Román María Eugenia , Schoebitz Mauricio , Bastías Richard M. , Fernández Pablo S. , García-Viguera Cristina , López-Belchi María Dolores
Published: January 2021

Calafate (Berberis microphylla G. Forst) is a wild bush plant widely distributed in the south of Argentina and Chile. Their blue colored fruits present particular flavor and health benefits attributed to high polyphenol contents biosynthesized by the plant under stress. Studies about correlation of abiotic conditions with anthocyanin profiles and physicochemical features of calafate beneath wild origin environment are not described yet. Hence, this research aimed to evaluate the physicochemical changes, antioxidant activity and anthocyanin content of calafate fruit in relationship to UV solar radiation (W.m−2) and air temperature (°C) environment condition during three consecutive years (2017, 2018, 2019). Variations in fruit anthocyanins were determined by comparison between high performance liquid chromatography (HPLC-DAD-ESI)/MSn and CIEL*a*b* colors parameters. Correlations were analyzed by principal component analysis (PCA). Radiation was negatively correlated with fruit size and weight. Physicochemical aspects such as pH, soluble solids, color, total anthocyanins, flavanols and other phenolic compounds were positively correlated with temperature changes. The quantities of monomeric anthocyanins were dependent on both low temperature and global radiation (reaching 20.01 mg g−1 FW in calafate fruit). These results constitute a valuable resource to understand the structural and physiological plasticity of calafate in facing climate changes for future domestication research as well as for agri-food industrial application.
Defining the Rhizobium leguminosarum Species Complex
Authors: Young J. Peter W. , Moeskjær Sara , Afonin Alexey , Rahi Praveen , Maluk Marta , James Euan K. , Cavassim Maria Izabel A. , Rashid M. Harun-or , Aserse Aregu Amsalu , Perry Benjamin J. , Wang En Tao , Velázquez Encarna , Andronov Evgeny E. , Tampakaki Anastasia , Flores Félix José David , Rivas González Raúl , Youseif Sameh H. , Lepetit Marc , Boivin Stéphane , Jorrin Beatriz , Kenicer Gregory J. , Peix Álvaro , Hynes Michael F. , Ramírez-Bahena Martha Helena , Gulati Arvind , Tian Chang-Fu
Published: January 2021

Bacteria currently included in Rhizobium leguminosarum are too diverse to be considered a single species, so we can refer to this as a species complex (the Rlc). We have found 429 publicly available genome sequences that fall within the Rlc and these show that the Rlc is a distinct entity, well separated from other species in the genus. Its sister taxon is R. anhuiense. We constructed a phylogeny based on concatenated sequences of 120 universal (core) genes, and calculated pairwise average nucleotide identity (ANI) between all genomes. From these analyses, we concluded that the Rlc includes 18 distinct genospecies, plus 7 unique strains that are not placed in these genospecies. Each genospecies is separated by a distinct gap in ANI values, usually at approximately 96% ANI, implying that it is a ‘natural’ unit. Five of the genospecies include the type strains of named species: R. laguerreae, R. sophorae, R. ruizarguesonis, “R. indicum” and R. leguminosarum itself. The 16S ribosomal RNA sequence is remarkably diverse within the Rlc, but does not distinguish the genospecies. Partial sequences of housekeeping genes, which have frequently been used to characterize isolate collections, can mostly be assigned unambiguously to a genospecies, but alleles within a genospecies do not always form a clade, so single genes are not a reliable guide to the true phylogeny of the strains. We conclude that access to a large number of genome sequences is a powerful tool for characterizing the diversity of bacteria, and that taxonomic conclusions should be based on all available genome sequences, not just those of type strains.
Whole Genome Analysis of Environmental Pseudomonas mendocina Strains: Virulence Mechanisms and Phylogeny
Authors: Ruiz-Roldán Lidia , de Toro María , Sáenz Yolanda
Published: January 2021

Pseudomonas mendocina is an environmental bacterium, rarely isolated in clinical specimens, although it has been described as producing endocarditis and sepsis. Little is known about its genome. Whole genome sequencing can be used to learn about the phylogeny, evolution, or pathogenicity of these isolates. Thus, the aim of this study was to analyze the resistome, virulome, and phylogenetic relationship of two P. mendocina strains, Ps542 and Ps799, isolated from a healthy Anas platyrhynchos fecal sample and a lettuce, respectively. Among all of the small number of P.mendocina genomes available in the National Center for Biotechnology Information (NCBI) repository, both strains were placed within one of two well-defined phylogenetic clusters. Both P. mendocina strains lacked antimicrobial resistance genes, but the Ps799 genome showed a MOBP3 family relaxase. Nevertheless, this study revealed that P. mendocina possesses an important number of virulence factors, including a leukotoxin, flagella, pili, and the Type 2 and Type 6 Secretion Systems, that could be responsible for their pathogenesis. More phenotypical and in vivo studies are needed to deepen the association with human infections and the potential P. mendocina pathogenicity.
Embryo Genetics
Authors: Rubio Carmen , Simón Carlos
Published: January 2021

Advances in embryo and reproductive genetics have influenced clinical approaches to overcome infertility. Since the 1990s, many attempts have been made to decipher the genetic causes of infertility and to understand the role of chromosome aneuploidies in embryo potential. At the embryo stage, preimplantation genetic testing for chromosomal abnormalities and genetic disorders has offered many couples the opportunity to have healthy offspring. Recently, the application of new technologies has resulted in more comprehensive and accurate diagnoses of chromosomal abnormalities and genetic conditions to improve clinical outcome. In this Special Issue, we include a collection of reviews and original articles covering many aspects of embryo diagnosis, genome editing, and maternal–embryo cross-communication during the implantation process.
Satellite DNA in Neotropical Deer Species
Authors: Vozdova Miluse , Kubickova Svatava , Martínková Natália , Galindo David Javier , Bernegossi Agda Maria , Cernohorska Halina , Kadlcikova Dita , Musilová Petra , Duarte Jose Mauricio , Rubes Jiri
Published: January 2021

The taxonomy and phylogenetics of Neotropical deer have been mostly based on morphological criteria and needs a critical revision on the basis of new molecular and cytogenetic markers. In this study, we used the variation in the sequence, copy number, and chromosome localization of satellite I-IV DNA to evaluate evolutionary relationships among eight Neotropical deer species. Using FISH with satI-IV probes derived from Mazama gouazoubira, we proved the presence of satellite DNA blocks in peri/centromeric regions of all analyzed deer. Satellite DNA was also detected in the interstitial chromosome regions of species of the genus Mazama with highly reduced chromosome numbers. In contrast to Blastocerus dichotomus, Ozotoceros bezoarticus, and Odocoileus virginianus, Mazama species showed high abundance of satIV DNA by FISH. The phylogenetic analysis of the satellite DNA showed close relationships between O. bezoarticus and B. dichotomus. Furthermore, the Neotropical and Nearctic populations of O. virginianus formed a single clade. However, the satellite DNA phylogeny did not allow resolving the relationships within the genus Mazama. The high abundance of the satellite DNA in centromeres probably contributes to the formation of chromosomal rearrangements, thus leading to a fast and ongoing speciation in this genus, which has not yet been reflected in the satellite DNA sequence diversification.
The Impact of the COVID-19 Pandemic on Gastrointestinal Endoscopy Activity in a Tertiary Care Center from Northeastern Romania
Authors: Chiriac Stefan , Stanciu Carol , Cojocariu Camelia , Sfarti Catalin , Singeap Ana-Maria , Girleanu Irina , Cuciureanu Tudor , Huiban Laura , David Diana , Zenovia Sebastian , Nastasa Robert , Balan Gheorghe G. , Trifan Anca
Published: January 2021

Background: The outbreak of the coronavirus disease 2019 (COVID-19) has led to significant changes in endoscopy units worldwide, with potential impact on patients’ welfare as well as on endoscopy training. We aimed to assess the real-life impact of COVID-19 on the endoscopy unit in a tertiary care center from Romania. Methods: A 6.5-month period during the COVID-19 pandemic was compared to a similar period from 2019. Results: A 6.2-fold decrease of endoscopic procedures was noted. Colonoscopies were reduced from 916 to 42, p < 0.001; flexible sigmoidoscopies from 189 to 14, p = 0.009; upper gastrointestinal (GI) endoscopies from 2269 to 401, p = 0.006; and ERCP from 234 to 125, p < 0.001. The percentage of emergency procedures increased (38.8% vs. 26.2%, p < 0.001), as well as the rate of endoscopies performed for upper GI bleeding (42.5% vs. 24.4%, respectively, p < 0.001). The detection of cancers was considerably reduced (57 compared to 249, p = 0.001). There were fewer complications and higher success rates (7.6% vs. 19.2%, p < 0.001, and 94.2% vs. 90.7%, respectively). Fellows participation was also reduced from 90% to 40.9% (p < 0.001). Conclusions: The COVID-19 pandemic has significantly altered the workflow of the endoscopy unit, lowering the number of procedures performed and potentially compromising the early detection of cancers.
What Will Be the Impact of the COVID-19 Quarantine on Psychological Distress? Considerations Based on a Systematic Review of Pandemic Outbreaks
Authors: Cavicchioli Marco , Ferrucci Roberta , Guidetti Matteo , Canevini Maria Paola , Pravettoni Gabriella , Galli Federica
Published: January 2021

Background: The novel coronavirus (SARS-CoV-2) and related syndrome (COVID-19) has led to worldwide measures with severe consequences for millions of people. In the light of the psychopathological consequences of restrictive measures detected during previous outbreaks, a systematic review was carried out to provide an evidence-based assessment of possible effects of the current COVID-19 quarantine on mental health. Methods: This review included studies that assessed mental health indexes (e.g., overall psychological distress, depressive and PTSD symptoms) during and after quarantine periods adopted to management different outbreaks (e.g., COVID-19, SARS, MERS). Results: Twenty-one independent studies were included for a total of 82,312 subjects. At least 20% of people exposed to restrictive measures for the management of pandemic infections reported clinically significant levels of psychological distress, especially PTSD (21%) and depressive (22.69%) symptoms. Overall, original studies highlighted relevant methodological limitations. Conclusions: Nowadays, almost one out of every five people is at risk of development of clinically significant psychological distress. Further research on mental health after the current COVID-19 quarantine measures is warranted.