bluuebunny/crossref_metadata_2025 huggingface dataset:
Current Insight into Traditional and Modern Methods in Fungal Diversity Estimates
Authors: Gautam Ajay Kumar , Verma Rajnish Kumar , Avasthi Shubhi , Sushma  , Bohra Yogita , Devadatha Bandarupalli , Niranjan Mekala , Suwannarach Nakarin
Published: February 2022

Fungi are an important and diverse component in various ecosystems. The methods to identify different fungi are an important step in any mycological study. Classical methods of fungal identification, which rely mainly on morphological characteristics and modern use of DNA based molecular techniques, have proven to be very helpful to explore their taxonomic identity. In the present compilation, we provide detailed information on estimates of fungi provided by different mycologistsover time. Along with this, a comprehensive analysis of the importance of classical and molecular methods is also presented. In orderto understand the utility of genus and species specific markers in fungal identification, a polyphasic approach to investigate various fungi is also presented in this paper. An account of the study of various fungi based on culture-based and cultureindependent methods is also provided here to understand the development and significance of both approaches. The available information on classical and modern methods compiled in this study revealed that the DNA based molecular studies are still scant, and more studies are required to achieve the accurate estimation of fungi present on earth.
The Nature of Work in the Media Industries: A Literature Review and Future Directions
Authors: Omidi Afshin , Dal Zotto Cinzia , Picard Robert G.
Published: February 2022

Media work is a culture-making activity affecting the ways people understand the world and, therefore, workers in the media industries have a critical role in shaping collective memories, traditions, and belief systems. While studies regarding the characteristics impacting the nature of work in the media industries have significantly been increasing over the last years, the literature in this area remains highly fragmented. This paper begins to address that shortcoming by conducting an in-depth review of 36 scholarly papers in influential journals published from 2006 to 2020 to provide a comprehensive view of the literature and its approaches. This study elaborates on the concept of media work by organizing previous efforts into five subthemes, including commonalities, contested terrain, gendered profession, emerging practices, and influencing factors. Previous research has emphasized that media workers’ subjective experiences need to be explored further and more in-depth; however, if we wish to depict a more holistic but realistic picture, those experiences should be contextualized and thus linked with the specific organizational configurations and macro structures in which media work is embedded. The present review depicts how work in the media may take different meanings when addressing it through various theoretical frameworks. Our study can enrich future studies regarding the nature of media work by providing a fine-grained foundation in which researchers could understand how their given research problem(s) would be connected with the other issues that potentially impact their studies.
Personalized Preoperative Prediction of the Length of Hospital Stay after TAVI Using a Dedicated Decision Tree Algorithm
Authors: Zisiopoulou Maria , Berkowitsch Alexander , Neuber Ralf , Gouveris Haralampos , Fichtlscherer Stephan , Walther Thomas , Vasa-Nicotera Mariuca , Seppelt Philipp
Published: February 2022

Background: The aim of this study was to identify pre-operative parameters able to predict length of stay (LoS) based on clinical data and patient-reported outcome measures (PROMs) from a scorecard database in patients with significant aortic stenosis who underwent TAVI (transfemoral aortic valve implantation). Methods: 302 participants (51.7% males, age range 78.2–84.2 years.) were prospectively recruited. After computing the median LoS value (=6 days, range = 5–8 days), we implemented a decision tree algorithm by setting dichotomized values at median LoS as the dependent variable and assessed baseline clinical variables and PROMs (Clinical Frailty Scale (CFS), EuroQol-5 Dimension-5 Levels (EQ-5D) and Kansas City Cardiomyopathy Questionnaire (KCCQ)) as potential predictors. Results: Among clinical parameters, only peripheral arterial disease (p = 0.029, HR = 1.826) and glomerular filtration rate (GFR, cut-off < 33 mL/min/1.73 m2, p = 0.003, HR = 2.252) were predictive of LoS. Additionally, two PROMs (CFS; cut-off = 3, p < 0.001, HR = 1.324 and KCCQ; cut-off = 30, p = 0.003, HR = 2.274) were strong predictors. Further, a risk score for LoS (RS_LoS) was calculated based on these predictors. Patients with RS_LoS = 0 had a median LoS of 5 days; patients RS_LoS ≥ 3 had a median LoS of 8 days. Conclusions: based on the pre-operative values of the above four predictors, a personalized prediction of LoS after TAVI can be achieved.
Combined Role of Fe Nanoparticles (Fe NPs) and Staphylococcus aureus L. in the Alleviation of Chromium Stress in Rice Plants
Authors: Alharby Hesham F. , Ali Shafaqat
Published: February 2022

Chromium (Cr) is a toxic heavy metal whose high concentration in soil badly affects plant growth, photosynthesis, and overall yield. Metal-derived nano-particles and metal-resistant bacteria can strengthen the plant defense system against different abiotic stresses; however, little is known about the use of nanoparticles in conjunction with bacteria. This study examined the combined effect of Fe nanoparticles (Fe NPs) and a chromium-resistant bacterium Staphylococcus aureus, on rice plants grown on chromium saturated medium. Chromium stress reduced rice growth, biomass, and chlorophyll contents by causing oxidative damage leading to overproduction of electrolyte leakage, hydrogen peroxide, and malondialdehyde. Fe NPs significantly improved plant growth, biomass, yield, and photosynthetic activity by enhancing the chlorophyll contents and alleviating oxidative damage. Application of Fe NPs also reduced the uptake and accumulation of Cr in the plants by increasing the bioavailability of micronutrients to the plant. The Fe NPs decreased oxidative damage and enhanced the enzymatic and non-enzymatic activity in the plant to withstand Cr stress compared to the plants without Fe NPs treatments. The inoculation of rice plants with the chromium-resistant bacteria S. aureus further enhanced the positive impact of Fe NPs by transforming the toxic form of chromium (Cr6+) into a less toxic form of chromium (Cr3+). The bacterial inoculation reduced Cr uptake by plants through adsorption of Cr ions, resulting in decreased chromium ion bioavailability. At chromium level 100 mg/kg, the foliar application of Fe NPs from 0 to 20 mg/L increased the total chlorophyll contents from 2.8 to 3.9. The application of S. aureus further enhanced the chlorophyll contents from 4.4 to 5.4, respectively. The current study suggested that combining Fe NPs and S. aureus could be a viable strategy for reducing Cr toxicity and accumulation in rice plants and most likely other plants.
Screening of Leafy Vegetable Varieties with Low Lead and Cadmium Accumulation Based on Foliar Uptake
Authors: Xu Zhangqian , Peng Jianwei , Zhu Zhen , Yu Pengyue , Wang Maodi , Huang Zhi , Huang Ying , Li Zhaojun
Published: February 2022

Leafy vegetables cultivated in kitchen gardens and suburban areas often accumulate excessive amounts of heavy metals and pose a threat to human health. For this reason, plenty of studies have focused on low accumulation variety screening. However, identifying specific leafy vegetable varieties according to the foliar uptake of air pollution remains to be explored (despite foliar uptake being an important pathway for heavy-metal accumulation). Therefore, in this study, the lead (Pb) and cadmium (Cd) contents, leaf morphology, and particle matter contents were analyzed in a micro-area experiment using 20 common vegetables. The results show that the Pb content in leaves ranged from 0.70 to 3.86 mg kg−1, and the Cd content ranged from 0.21 to 0.99 mg kg−1. Atmospheric particles were clearly scattered on the leaf surface, and the particles were smaller than the stomata. Considering the Pb and Cd contents in the leaves and roots, stomata width-to-length ratio, leaf area size, enrichment factor, and translocation factor, Yidianhongxiancai, Qingxiancai, Baiyuanyexiancai, Nanjingjiangengbai and Sijixiaobaicai were recommended for planting in kitchen gardens and suburban areas as they have low accumulation characteristics. Identifying the influencing factors in the accumulation of heavy metals in vegetables through foliar uptake is important to help plant physiologists/environmentalists/policy makers to select suitable varieties for planting in air-polluted areas and thus reduce their threat to human health.
The Anti-Endometriotic Effect of Cyperi Rhizoma Extract, Inhibiting Cell Adhesion and the Expression of Pain-Related Factors through Akt and NF-kB Pathways
Authors: Ahn Ji-Hye , Choi Jun-Myeong , Kang Eun-Sol , Yoo Jae-Hyeon , Cho Yoon-Jin , Jang Dae Sik , Choi Jung-Hye
Published: February 2022

Rhizomes of Cyperus rotundus have been widely used as a traditional medicine in Asia for the treatment of gynecological diseases. However, there is no scientific evidence demonstrating the effect of C. rotundus rhizomes on endometriosis, which is characterized by the adhesion of endometrial tissues outside the uterus, resulting in chronic and severe pelvic pain. The aim of this study was to investigate the effects of Cyperi rhizoma extract (CRE) on cell adhesion and the expression of pain-related factors (neurotrophins) in endometriotic cells, and to elucidate the underlying molecular mechanisms. CRE inhibited the adhesion of human endometriotic 12Z cells to peritoneal mesothelial Met5A cells using by adhesion assays. The mRNA expression of adhesion molecules [P-cadherin and matrix metalloproteinase (MMP)-2] was downregulated by CRE treatment. In addition, CRE significantly inhibited the mRNA expression of neurotrophins (BDNF, NGF, NT-3 and NT-4/5) in 12Z cells. Moreover, Akt overexpression markedly neutralized the inhibition of cell adhesion by CRE and expression of neurotrophins in 12Z cells. Furthermore, it was found that CRE suppressed NF-kB activation through the Akt pathway. These data suggest that CRE exerts anti-endometriotic activities by the inhibition of cell adhesion and neurotrophin expression, through the negative regulation of the Akt and NF-kB pathways in endometriotic cells.
Improved Fixation Stability of a Dedicated Rib Fixation System in Flail Chest: A Retrospective Study
Authors: Tsai Shang-Ting , Lin Hung-Yu , Li Chia-Ying , Lin Chih-Chien
Published: February 2022

Background and Objectives: Flail chest typically results from major trauma to the thoracic cage and is accompanied by multiple rib fractures. It has been well documented that surgical fixation of rib fractures can decrease both morbidity and mortality rates. This study aimed to evaluate the effectiveness of a dedicated APS Rib Fixation System, which features a pre-contoured design based on anatomical rib data of the Asian population. Materials and Methods: We reviewed 43 consecutive patients, who underwent surgical stabilization for flail chest with the traditional Mini bone plate (n = 20), APS plate (n = 13), or Mini + APS (n = 10). Demographic and injury variables were documented. We used X-ray radiography to determine plate fractures and screw dislocations after surgical fixation. Results: No statistical differences were noted in the demographic or injury variables. APS plates demonstrated fewer cases of plate fractures and screw dislocations than Mini plates (OR = 0.091, p = 0.008). Conclusions: The pre-contoured design of the APS plate demonstrated a superior rib implant failure rate as compared to the traditional Mini bone plate. Our study indicates that the APS plate may serve as an effective surgical tool for the treatment of flail chest.
Surface Hardening Behavior of Advanced Gear Steel C61 by a Novel Solid-Solution Carburizing Process
Authors: Dai Yanzhang , Kang Lixia , Han Shun , Li Yong , Liu Yu , Lei Simin , Wang Chunxu
Published: February 2022

During vacuum carburizing, coarse reticulated carbides tend to precipitate along grain boundaries due to high-carbon-potential conditions. This phenomenon is often one of the main factors in the failure of conventional gear steels. In this paper, a novel solid-solution carburizing process was proposed to achieve nano-carbide formation in the surface of the carburizing layer, and the conventional carburizing process and material thermodynamic calculations were combined to study the carburized layer by changing the parameters of the carburizing process, and to optimize the microstructure and properties of the carburized layer. The results showed that the high carbon potential or the long-time boost carburizing process could easily cause the enrichment of many carbon atoms in the traditional carburization, thus forming a carbide network and decreasing the carburization efficiency. The minor increase in large-sized M7C3 carbides did not significantly improve the surface hardness and wear resistance. However, the presence of small and dispersed M2C carbides was the main factor in improving the microhardness and mechanical properties. The novel solid-solution carburizing process could improve the carburizing efficiency and transform reticulated carbides into nano-dispersed M2C carbides. The surface carbon content and microhardness of 1.07% and 875 HV, respectively, increased 17.7 and 2.4% compared to conventional carburizing processes at 1100 °C. On the other hand, the surface’s ultimate tensile strength was found to be 1900 MPa by mini-tensile testing, and the core had a good match of strength and toughness. It was concluded that the novel solid-solution carburizing process could dissolve the carbon network and thus effectively increase the surface carbon content, achieving fully nanosized carbide on the surface. Modifying the size, morphology, and distribution of the nano-M2C carbides dispersed within the lath-martensite after tempering the test steel was found to be the main factor in improving the mechanical properties.
Corrosion Behavior of Heat-Treated Nickel-Aluminum Bronze and Manganese-Aluminum Bronze in Natural Waters
Authors: Cobo Ocejo Ignacio , Biezma Moraleda Maria Victoria , Linhardt Paul
Published: February 2022

Nickel Aluminum Bronze (NAB) and Manganese Aluminum Bronze (MAB) are high-alloyed bronzes that are increasingly employed in several industrial sectors, mainly related to hostile environments due to their excellent properties against corrosion, cavitation, erosion and improved mechanical properties in relation to other copper-based alloys. These materials are very sensitive against thermal treatments due to a multiphase microstructure in as-cast condition. To contribute to the knowledge of the behavior of both alloys, the effect of thermal treatments on the corrosion behavior of NAB (CuAl10Fe5Ni5) and MAB (CuMn12Al8Fe4Ni2) was studied. As-cast material was subjected to various combinations of quenching and quenching and tempering at 850 °C and 600 °C. Corrosion testing was carried out using simulated sea and fresh water. The microstructures of the as-cast and heat-treated samples were characterized by metallography using two chemical agents with FeCl3 and NH4OH solutions and examination by optical and scanning electron microscopy. The major effect of thermal treatments on corrosion was found in influencing the amount and distribution of β-phase, which is prone to selective corrosion in both electrolytes.
Study on SPF/DB Technology for Two-Sheet Hollow Structure of 1420 Al-Li Alloy
Authors: Li Yaoming , Wang Honglin , Niu Tao , Zhang Huang , Yuan Meini
Published: February 2022

The two-sheet hollow structure of the 1420 Al-Li alloy was prepared by the method of superplastic forming and diffusion bonding. The interface combination status of the diffusion bonding region and the microstructure of the superplastic forming region were observed by an optical microscope. The thickness distribution of the superplastic forming region was measured by an ultrasonic thickness meter machine, the defect detection was tested by X-ray nondestructive inspection, and the failure modes of the samples were analyzed. The results showed that the two-sheet hollow structure of the 1420 Al-Li alloy was prepared successfully, the structure was integrated, and there were no shape defects such as pit, wrinkle, and collapse. The structure shape was almost attached to the die completely, and the thickness was almost uniform distribution. The no deforming area of the two-sheet hollow structure of the 1420 Al-Li alloy was a long strip, rolled microstructure, while the grains near the round corner area were equiaxed states resulting from dynamic recrystallization. The improper control of the superplastic gas pressure in the forming process would lead to the tearing or the die-attaching failure for the two-sheet hollow structure of the 1420 Al-Li alloy.
Effect of Shortened Post Weld Heat Treatment on the Laser Welded AA7075 Alloy
Authors: Suckow Timon , Völkers Stephan , Bütev Öcal Ezgi , Grass Markus , Böhm Stefan , Groche Peter
Published: February 2022

The present study provides an overview of previous studies on the welding of the AA7075-T6 aluminum alloy, followed by an investigation of the influence of short-time solution annealing on the mechanical properties of the weld. Conventional laser welding of Al-Zn-alloys leads to a low weld strength, which makes a post weld heat treatment (PWHT) favorable. The PWHT includes solution annealing, quenching and subsequent aging. For solution annealing, different holding times and cooling rates are investigated in this study. The focus of the investigation is on a short solution annealing time, which on the one hand is ecological and economically favorable and on the other hand offers great potential for inline heat treatments. The shortest solution annealing time of 10 s shows a significant increase in weld strength (joint efficiency of 72%), compared to the non-heat treated weld (joint efficiency of 52%). The microstructural analysis reveals that the cooling rate after solution heat treatment affects the formation of precipitates in the microstructure of the welded AA7075 alloy. Moreover, the enhancement of mechanical properties is related to the formation of Mg-Al-Cu and Mg-Zn rich precipitates.
Mechanical Aspects of Nonhomogeneous Deformation of Aluminum Single Crystals under Compression along [100] and [110] Directions
Authors: Romanova Varvara , Balokhonov Ruslan , Zinovieva Olga , Lychagin Dmitry , Emelianova Evgeniya , Dymnich Ekaterina
Published: February 2022

The deformation behavior of aluminum single crystals subjected to compression along the [100] and [110] directions is numerically examined in terms of crystal plasticity. A constitutive model taking into account slip geometry in face-centered cubic crystals is developed using experimental data for the single-crystal samples with lateral sides coplanar to certain crystal planes. Two sets of calculations are performed using ABAQUS/Explicit to examine the features of plastic strain evolution in perfectly plastic and strain-hardened crystals. Special attention is given to the discussion of mechanical aspects of crystal fragmentation. Several distinct deformation stages are revealed in the calculations. In the first stage, narrow solitary fronts of plastic deformation are alternately formed near the top or bottom surfaces and then propagate towards opposite ends to save the symmetry of the crystal shape. The strain rate within the fronts is an order of magnitude higher than the average strain rate. The first stage lasts longer in the strain-hardened crystals, eventually giving way to an intermediate stage of multiple slips in different crystal parts. Finally, the crystal shape becomes asymmetrical, but no pronounced macroscopic strain localization has been revealed at any deformation stage. The second stage in perfectly plastic crystals relates to abrupt strain localization within a through-thickness band-shaped region, accompanied by macroscale crystal fragmentation. Stress analysis has shown that pure compression took place only in the first deformation stage. Once the crystal shape has lost its symmetry, the compressive stress in some regions progressively decreases to zero and eventually turns tensile.
Comparative Transcriptome and Phytochemical Analysis Provides Insight into Triterpene Saponin Biosynthesis in Seeds and Flowers of the Tea Plant (Camellia sinensis)
Authors: Chen Cong , Zhu Huanqing , Kang Jiaxin , Warusawitharana Hasitha Kalhari , Chen Shuna , Wang Kaixi , Yu Fei , Wu Yuanyuan , He Puming , Tu Youying , Li Bo
Published: February 2022

Triterpene saponins exhibit various biological and pharmacological activities. However, the knowledge on saponin biosynthesis in tea plants (Camellia sinensis L.) is still limited. In this work, tea flower and seed samples at different developmental stages and leaves were collected and analyzed with UPLC-PDA-MS and RNA sequencing for saponin determination and transcriptome comparison. The saponin content reached around 19% in the freshly mature seeds and 7% in the green flower buds, and decreased with the fruit ripeness and flower blooming. Almost no saponins were detected in leaf samples. PCA and KEGG analysis suggested that the gene expression pattern and secondary metabolism in TF1 and TS2 vs. leaf samples were significantly different. Weighted gene coexpression network analysis (WGCNA) uncovered two modules related to saponin content. The mevalonate (MVA) instead of 2-C-methyl-d-erythritol-4-phospate (MEP) pathway was responsible for saponin accumulation in tea plants, and 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), diphosphomevalonate decarboxylase (MVD) and isopentenyl diphosphate isomerase (IDI) may be the key enzymes involved in saponin biosynthesis in tea seeds and flowers. Moreover, ten transcription factors (TFs) were predicted to regulate saponin biosynthesis in the tea plant. Taken together, our study provides a global insight into the saponin biosynthesis and accumulation in the tea plant.
Age, Sex, Body Mass Index, Diet and Menopause Related Metabolites in a Large Homogeneous Alpine Cohort
Authors: Verri Hernandes Vinicius , Dordevic Nikola , Hantikainen Essi Marjatta , Sigurdsson Baldur Bragi , Smárason Sigurður Vidir , Garcia-Larsen Vanessa , Gögele Martin , Caprioli Giulia , Bozzolan Ilaria , Pramstaller Peter P. , Rainer Johannes
Published: February 2022

Metabolomics in human serum samples provide a snapshot of the current metabolic state of an individuum. Metabolite concentrations are influenced by both genetic and environmental factors. Concentrations of certain metabolites can further depend on age, sex, menopause, and diet of study participants. A better understanding of these relationships is pivotal for the planning of metabolomics studies involving human subjects and interpretation of their results. We generated one of the largest single-site targeted metabolomics data sets consisting of 175 quantified metabolites in 6872 study participants. We identified metabolites significantly associated with age, sex, body mass index, diet, and menopausal status. While most of our results agree with previous large-scale studies, we also found novel associations including serotonin as a sex and BMI-related metabolite and sarcosine and C2 carnitine showing significantly higher concentrations in post-menopausal women. Finally, we observed strong associations between higher consumption of food items and certain metabolites, mostly phosphatidylcholines and lysophosphatidylcholines. Most, and the strongest, relationships were found for habitual meat intake while no significant relationships were found for most fruits, vegetables, and grain products. Summarizing, our results reconfirm findings from previous population-based studies on an independent cohort. Together, these findings will ultimately enable the consolidation of sets of metabolites which are related to age, sex, BMI, and menopause as well as to participants’ diet.
Microelectromechanical Systems Based on Magnetic Polymer Films
Authors: Ficai Denisa , Gheorghe Marin , Dolete Georgiana , Mihailescu Bogdan , Svasta Paul , Ficai Anton , Constantinescu Gabriel , Andronescu Ecaterina
Published: February 2022

Microelectromechanical systems (MEMS) have been increasingly used worldwide in a wide range of applications, including high tech, energy, medicine or environmental applications. Magnetic polymer composite films have been used extensively in the development of the micropumps and valves, which are critical components of the microelectromechanical systems. Based on the literature survey, several polymers and magnetic micro and nanopowders can be identified and, depending on their nature, ratio, processing route and the design of the device, their performances can be tuned from simple valves and pumps to biomimetic devices, such as, for instance, hearth ventricles. In many such devices, polymer magnetic films are used, the disposal of the magnetic component being either embedded into the polymer or coated on the polymer. One or more actuation zones can be used and the flow rate can be mono-directional or bi-directional depending on the design. In this paper, we review the main advances in the development of these magnetic polymer films and derived MEMS: microvalve, micropump, micromixer, microsensor, drug delivery micro-systems, magnetic labeling and separation microsystems, etc. It is important to mention that these MEMS are continuously improving from the point of view of performances, energy consumption and actuation mechanism and a clear tendency in developing personalized treatment. Due to the improved energy efficiency of special materials, wearable devices are developed and be suitable for medical applications.
Hybrid Dissection for Neutron Tube Shell via Continuous-Wave Laser and Ultra-Short Pulse Laser
Authors: Kang Minqiang , Qiang Yongfa , Zhu Canlin , Xiang Xiangjun , Zhou Dandan , Peng Zhitao , Xie Xudong , Zhu Qihua
Published: February 2022

The sealed neutron tube shell dissection process utilizing the traditional lathe turning method suffers from low efficiency and high cost due to the frequency of replacement of the diamond knife. In this study, a hybrid dissection method is introduced by combining the continuous-wave (CW) laser for efficient tangential groove production with an ultra-short pulse laser for delamination scanning removal. In this method, a high-power CW laser is firstly employed to make a tapered groove on the shell’s surface, and then a femtosecond pulse laser is used to micromachine the groove in order to obtain a cutting kerf. The thermal field was theoretically investigated in a finite element model. The simulation results show that the width of the area of temperature exceeding 100 °C is 1.9 mm and 0.4 mm with rotating speeds of 20 rad/s and 60 rad/s, respectively. In addition, a 2 mm deep slot in the 25 mm diameter tube was successfully produced in 1 min by a kilowatt fiber laser, and a 500-femtosecond pulse laser was employed to cut a plate with a material removal rate of 0.2 mm3/min. By using the hybrid method, the cutting efficiency was improved about 49 times compared to the femtosecond laser cutting. According to the simulation and experimental results, this method provides a high-efficiency and non-thermal cutting technique for reclaimed metallic neutron tube shells with millimeter-level thick walls, which has the advantages of non-contact, minimal thermal diffusion, and no effect of molten slag. It is indicated that the hybrid dissection method not only offers a new solution for thick neutron tube shell cutting but also extends the application of laser cutting techniques.
Reconfigurable Angular Resolution Design Method in a Separate-Axis Lissajous Scanning MEMS LiDAR System
Authors: Xu Fahu , Qiao Dayong , Xia Changfeng , Song Xiumin , Zheng Wenhui , He Yaojun , Fan Qiaodan
Published: February 2022

MEMS-based LiDAR with a low cost and small volume is a promising solution for 3D measurement. In this paper, a reconfigurable angular resolution design method is proposed in a separate-axis Lissajous scanning MEMS LiDAR system. This design method reveals the influence factors on the angular resolution, including the characteristics of the MEMS mirrors, the laser duty cycle and pulse width, the processing time of the echo signal, the control precision of the MEMS mirror, and the laser divergence angle. A simulation was carried out to show which conditions are required to obtain different angular resolutions. The experimental results of the 0.2° × 0.62° and 0.2° × 0.15° (horizontal × vertical) angular resolutions demonstrate the feasibility of the design method to realize a reconfigurable angular resolution in a separate-axis Lissajous scanning MEMS LiDAR system by employing MEMS mirrors with different characteristics. This study provides a reasonable potential to obtain a high and flexible angular resolution for MEMS LiDAR.
A MEMS Electrochemical Seismometer Based on the Integrated Structure of Centrosymmetric Four Electrodes
Authors: Duan Yumo , Zhong Anxiang , Lu Yulan , Chen Jian , Chen Deyong , Wang Junbo
Published: February 2022

This paper presented an electrochemical seismic micro sensor based on an integrated structure of four centrosymmetric electrodes. In this integrative structure, cathodes were not only distributed on wafer surfaces but also on the inner walls of the flow holes of the wafer, which increased the effective cathode areas and improved the sensitivity of the sensor. Numerical simulations were conducted to validate the feasibility of the integrated structure of four centrosymmetric electrodes in monitoring seismic vibrations where variations in the arrangements of the flow holes and anode width were investigated. The integrated structure of the four centrosymmetric micro electrodes was fabricated based on Micro-Electro-Mechanical Systems (MEMS) without the requirement of manual alignments. Experimental characterizations revealed that: (1) the maximum sensitivity of the electrochemical seismic sensor based on the integrated structure of four centrosymmetric electrodes was two orders of magnitude higher than that of the commercial counterpart of CME6011 and three times higher than the electrochemical seismic sensor based on the integrated structure of four planar micro electrodes; (2) the electrochemical seismic sensor based on the integrated structure of four centrosymmetric micro electrodes demonstrated comparable and even lower noise levels in comparison to CME6011. Thus, the electrochemical seismic micro sensor developed in this study may function as an enabling tool in future applications of seismic monitoring and geophysical explorations.
Normalization of Blood Viscosity According to the Hematocrit and the Shear Rate
Authors: Trejo-Soto Claudia , Hernández-Machado Aurora
Published: February 2022

The rheological properties of blood depend highly on the properties of its red blood cells: concentration, membrane elasticity, and aggregation. These properties affect the viscosity of blood as well as its shear thinning behavior. Using an experimental analysis of the interface advancement of blood in a microchannel, we determine the viscosity of different samples of blood. In this work, we present two methods that successfully normalize the viscosity of blood for a single and for different donors, first according to the concentration of erythrocytes and second according to the shear rate. The proposed methodology is able to predict the health conditions of the blood samples by introducing a non-dimensional coefficient that accounts for the response to shear rate of the different donors blood samples. By means of these normalization methods, we were able to determine the differences between the red blood cells of the samples and define a range where healthy blood samples can be described by a single behavior.
Molecular Mechanism of Overcoming Host Resistance by the Target of Rapamycin Gene in Leptographium qinlingensis
Authors: An Huanli , Gan Tian , Tang Ming , Chen Hui
Published: February 2022

Leptographium qinlingensis is a fungal symbiont of the Chinese white pine beetle (Dendroctonus armandi) and a pathogen of the Chinese white pine (Pinus armandii) that must overcome the terpenoid oleoresin defenses of host trees to invade and colonize. L. qinlingensis responds to monoterpene flow with abundant mechanisms that include the decomposing and use of these compounds as a nitrogen source. Target of Rapamycin (TOR) is an evolutionarily conserved protein kinase that plays a central role in both plants and animals through integration of nutrients, energies, hormones, growth factors and environmental inputs to control proliferation, growth and metabolism in diverse multicellular organisms. In this study, in order to explore the relationship between TOR gene and carbon sources, nitrogen sources, host nutrients and host volatiles (monoterpenoids) in L. qinlingensis, we set up eight carbon source treatments, ten nitrogen source treatments, two host nutrients and six monoterpenoids (5%, 10% and 20%) treatments, and prepared different media conditions. By measuring the biomass and growth rate of mycelium, the results revealed that, on the whole, the response of L. qinlingensis to nitrogen sources was better than carbon sources, and the fungus grew well in maltose (carbon source), (NH4)2C2O4 (inorganic nitrogen source), asparagine (organic nitrogen source) and P. armandii (host nutrient) versus other treatments. Then, by analyzing the relationship between TOR expression and different nutrients, the data showed that: (i) TOR expression exhibited negative regulation in response to carbon sources and host nutrition. (ii) The treatments of nitrogen sources and terpenoids had positively regulatory effects on TOR gene; moreover, the fungus was most sensitive to β-pinene and 3-carene. In conclusion, our findings reveal that TOR in L. qinlingensis plays a key role in the utilization of host volatiles as nutrient intake, overcoming the physical and chemical host resistances and successful colonization.
Neutron Activation Analysis in Urban Geochemistry: Impact of Traffic Intensification after Opening the Blanka Tunnel Complex in Prague
Authors: Mizera Jiří , Havelcová Martina , Machovič Vladimír , Borecká Lenka , Vöröš Dominik
Published: February 2022

Opening of the Blanka Tunnel Complex (BTC) in Prague, Czech Republic, the longest city tunnel in Europe, significantly increased traffic on a connected main road, the V Holešovičkách street (VHS). To study environmental changes in VHS connected with BTC opening, sampling of road dust and airborne particulate matter in the VHS vicinity started 3 years before BTC opening and continued until BTC pilot operation. The enrichment factors calculated for the collected samples from elemental compositions determined by instrumental neutron activation analysis (INAA) have shown significant enrichment for Se, Sb, Hg, Br, Mo, Zn, As, W, Cr, Ba, and Fe, but not their increase after starting BTC operation. The Principal Component Analysis allowed distinguishing between detrital and anthropogenic, probably traffic related, origin of the elements determined in samples. The study demonstrated a potential of INAA for multielement trace analysis of minute samples (28 elements determined in samples down to 20 μg, with detection limits down to sub-ng level) and its application in urban geochemistry studies.
Archaeometric Study of the White Marbles from “Madonna Della Febbre” Altar in San Domenico Church (Cosenza, Southern Italy)
Authors: Rovella Natalia , Bosco Stefania , Barca Donatella
Published: February 2022

San Domenico Church was built between 1441 and 1468 and represents one of the most important historical buildings of the Cosenza area (Calabria, Southern Italy) thanks to its architectonic style and the works inside, such as the “Madonna della Febbre”, a notable marble altar dated back to the XVI century. The church, as well as the sculptural group, underwent various interventions over time, unfortunately scarcely documented; thus, in this paper, the characterization of six white marble samples coming from the altar, was carried out to determine their provenance. The samples were analyzed by means of complementary methodologies well known in the archaeometric field: polarized optical microscopy (POM); an electron probe micro analyzer coupled with an energy dispersive spectrometer (EPMA-EDS); inductively coupled plasma–mass spectrometry (ICP-MS); and isotope ratio mass spectrometry (IRMS). The results provided important information about the “Madonna della Febbre” altar, suggesting the presence of different typologies of marbles and hypothesizing their possible provenance, including Carrara and Docimium. It was not clear if these marbles were re-used materials but, regardless, the reported information adds precious elements to the history of the entire architectonic complex, providing new issues to be deepened.
Characterization of Wall Paintings of the Harem Court in the Alhambra Monumental Ensemble: Advantages and Limitations of In Situ Analysis
Authors: Arjonilla Paz , Domínguez-Vidal Ana , Rubio Domene Ramón , Correa Gómez Elena , de la Torre-López María José , Ayora-Cañada María José
Published: February 2022

Non-invasive techniques (X-ray fluorescence, XRF, and Raman spectroscopy) were used for the study of the Hispano Muslim wall paintings. Principal component analysis (PCA) was performed on the semi-quantitative XRF results directly provided by the in-built factory calibrations with minimum user manipulation. The results obtained were satisfactory and highlighted differences and similarities among the measurement points. In this way, it was possible to differentiate the decorations carried out on gypsum plasterwork and the wall paintings over lime plaster. The color palette, revealed by combining the results from XRF and Raman spectroscopies, comprised the pigments hematite, lapis lazuli, cinnabar (in poor conservation state), and possibly, carbon. Evidence of past interventions was also provided by PCA on XRF data, which detected the presence of Pb, Ba, and Zn in some areas. Furthermore, the preparation layers have been studied in detail on cross-sections of two microsamples. Several layers of lime plaster with a compact microstructure have been observed. The characteristic of the pictorial layer and the identification of calcium oxalate point to the use of a secco-technique. The main alteration identified was a gypsum surface layer covering the painting and signs of plaster deterioration due to gypsum migration to more internal areas. Finally, the comparison with the observations made by restorers in previous interventions on these paintings revealed the importance of the representativeness gained with the in situ study, which enabled the analysis of a high number of areas.
Investigating the Neurotoxic Impacts of Arsenic and the Neuroprotective Effects of Dictyophora Polysaccharide Using SWATH-MS-Based Proteomics
Authors: Zhang Jun , Hu Ting , Wang Yi , Zhang Xinglai , Zhang Huajie , Lin Jing , Tang Xiaoxiao , Liu Xukun , Chen Margy , Khan Naseer Ullah , Shen Liming , Luo Peng
Published: February 2022

Arsenic (As) is one of the most important toxic elements in the natural environment. Currently, although the assessment of the potential health risks of chronic arsenic poisoning has received great attention, the research on the effects of arsenic on the brain is still limited. It has been reported that dictyophora polysaccharide (DIP), a common bioactive natural compound found in dietary plants, could reduce arsenic toxicity. Following behavioral research, comparative proteomics was performed to explore the molecular mechanism of arsenic toxicity to the hippocampi of SD (Sprague Dawley) rats and the protective effect of DIP. The results showed that exposure to arsenic impaired the spatial learning and memory ability of SD rats, while DIP treatment improved both the arsenic-exposed rats. Proteomic analysis showed that arsenic exposure dysregulated the expression of energy metabolism, apoptosis, synapse, neuron, and mitochondria related proteins in the hippocampi of arsenic-exposed rats. However, DIP treatment reversed or restored the expression levels of these proteins, thereby improving the spatial learning and memory ability of arsenic-exposed rats. This study is the first to use high-throughput proteomics to reveal the mechanism of arsenic neurotoxicity in rats as well as the protective mechanism of DIP against arsenic neurotoxicity.
Utilization of Hematite Particles for Economical Removal of o-xylene in a High-Temperature Gas-Solid Reactor
Authors: Ma Xiaolong , Zhao Dandan , Qian Jinjin , Ma Zichuan , Cui Jiansheng
Published: February 2022

To establish a novel approach for VOCs resource utilization, coupled o-xylene oxidation and hematite reduction was investigated in this study in a high-temperature gas-solid reactor in the temperature range 300–700 °C. As the o-xylene-containing inert gas (N2) stream traveled through the hematite particle bed, its reaction behavior was determined in programmed heating and constant temperature modes. Consequently, the effect of bed temperature, flow rate and o-xylene inlet concentration on both o-xylene removal performance and degree of hematite reduction was studied. The raw hematite and solid products were analyzed by TGA, XRF, XRD and SEM-EDS. The results showed that a temperature above 300 °C was required to completely eliminate o-xylene by hematite, and both o-xylene removal capacity and degree of hematite reduction at 5% breakthrough points enhanced on increasing the temperature and decreasing the flow rate. The increment in temperature from 300 °C to 700 °C led to a gradual reduction of Fe2O3 to Fe3O4, FeO and metallic iron. Thus, this study provides a novel, economic and promising technology for treating the VOC pollutants.
In Silico Molecular Docking and Simulation Studies of Protein HBx Involved in the Pathogenesis of Hepatitis B Virus-HBV
Authors: Shaikh Ibrahim Ahmed , Muddapur Uday M. , C Krithika , Badiger Shrikanth , Kulkarni Madhura , Mahnashi Mater H. , Alshamrani Saleh A. , Huneif Mohammed A. , More Sunil S. , Khan Aejaz Abdullatif , Iqubal S. M. Shakeel
Published: February 2022

Current drug discovery involves finding leading drug candidates for further development. New scientific approaches include molecular docking, ADMET studies, and molecular dynamic simulation to determine targets and lead compounds. Hepatitis B is a disease of concern that is a life-threatening liver infection. The protein considered for the study was HBx. The hepatitis B X-interacting protein crystal structure was obtained from the PDB database (PDB ID-3MSH). Twenty ligands were chosen from the PubChem database for further in silico studies. The present study focused on in silico molecular docking studies using iGEMDOCK. The triethylene glycol monoethyl ether derivative showed an optimum binding affinity with the molecular target HBx, with a high negative affinity binding energy of −59.02 kcal/mol. Lipinski’s rule of five, Veber, and Ghose were followed in subsequent ADMET studies. Molecular dynamic simulation was performed to confirm the docking studies and to analyze the stability of the structure. In these respects, the triethylene glycol monoethyl ether derivative may be a promising molecule to prepare future hepatitis B drug candidates. Substantial research effort to find a promising drug for hepatitis B is warranted in the future.
Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
Authors: Zhou Jie , Liang Meng , Lin Yu , Pang Hao , Wei Yutuo , Huang Ribo , Du Liqin
Published: February 2022

Resveratrol, an ingredient of traditional Chinese medicine, has beneficial effects on human health and huge potential for application in modern medicine. Polydatin is extracted from plants and then deglycosylated into resveratrol; enzymatic methods are preferred for this reaction. In this study, a β-D-glucosidase from Sphingomonas showed high efficiency in transforming polydatin into resveratrol and was tolerant toward organic solvents. Applying this enzyme in a biphasic transformation system resulted in 95.3% conversion of 20% concentration crude polydatin to resveratrol in 4 h. We thus report a new method for high-efficiency, clean production of resveratrol.
The Effect of Crataegus Fruit Pre-Treatment and Preservation Methods on the Extractability of Aroma Compounds during Liqueur Production
Authors: Tabaszewska Małgorzata , Najgebauer-Lejko Dorota , Zbylut-Górska Maria
Published: February 2022

The leaves, inflorescences, and fruits of hawthorn have long been known for their therapeutic properties. A wide range of hawthorn products, including liqueurs, are manufactured, due to the technological potential of the raw material as well as the richness of its volatile compounds. This study aimed to determine the effect of the liqueur production method and various methods of fruit preservation on the quantitative and qualitative composition of volatile compounds in the liqueurs produced. Hawthorn fruits saturated with sucrose and non-saturated with sucrose, fresh or preserved through one of three methods: freezing, air-drying, and freeze-drying, were used for liqueur preparation. The samples were analyzed using a gas chromatograph–mass spectrometer. They were found to contain 54 volatile compounds classified into 12 groups of chemicals. All 54 identified volatile compounds were detected in the liqueur made from hawthorn fruits non-saturated with sucrose and preserved by freeze-drying. In this liqueur type, 12 of the identified volatile compounds occurred in the highest concentration when compared to the other treatments. Among all volatiles, the following compounds were present in the analyzed liqueurs in the highest concentrations: dodecanoic acid ethyl ester (11.782 g/100 g), lactones (6.954 g/100 g), five monoterpenes (3.18 g/100 g), two aromatic hydrocarbons (1.293 g/100 g), isobensofuran (0.67 g/100 g), alcohol—2-methyl-2-propanol (0.059 g/100 g), and malonic ester (0.055 g/100 g). Among all analyzed liqueurs, the one made from the fruits non-saturated with sucrose and frozen was characterized by the smallest diversity of volatiles, which were present in the lowest concentrations in that liqueur.