bluuebunny/crossref_metadata_2025 huggingface dataset:
Caring Climate and Support, Mental Health, and Academic Adjustment: Effects from a Cluster Randomized Controlled Trial in Upper Secondary Schools in Norway
Authors: Larsen Torill Bogsnes , Urke Helga Bjørnøy , Kristensen Sara Madeleine , Mathisen Frida Kathrine Sofie
Published: November 2023

This cluster randomized controlled trial (RCT) examined the effect of a three year follow up of a multi-tier intervention aiming at improving the psychosocial environment in upper secondary schools in Norway. Two intervention conditions were tested: a universal single-tier intervention focused on improving the psychosocial school climate, the dream school program (DSP), and a multi-tier intervention combining the DSP with a targeted measure, the mental health support team (MHST). A total of 2203 students responded to the baseline survey. Of the 2203 pupils, 1884 responded to the first follow-up survey (year 1), 1287 pupils to the second (year 2), and 756 pupils to the third (year 3). The direct and indirect effects on school completion were analyzed using a multi-level linear mixed model. The results showed no significant effects of either the DSP or the DSP and MHST in combination on support, the school climate, mental health, or academic adjustment. We found no significant effect of the interventions on the proportion who had completed school or were in training (the three groups varied between 76.6% and 77.8%). Future similar studies should be attentive to the potential challenges of implementing RCTs in the school setting. Furthermore, the long-term effects of school interventions on the constructs included in this study could be difficult to capture due to the complexity of the phenomena. The implications of these findings are discussed.
CRISPR-Cas9-Based Gene Knockout of Immune Checkpoints in Expanded NK Cells
Authors: Mohammadian Gol Tahereh , Kim Miso , Sinn Ralph , Ureña-Bailén Guillermo , Stegmeyer Sarah , Gratz Paul Gerhard , Zahedipour Fatemeh , Roig-Merino Alicia , Antony Justin S. , Mezger Markus
Published: November 2023

Natural killer (NK) cell immunotherapy has emerged as a novel treatment modality for various cancer types, including leukemia. The modulation of inhibitory signaling pathways in T cells and NK cells has been the subject of extensive investigation in both preclinical and clinical settings in recent years. Nonetheless, further research is imperative to optimize antileukemic activities, especially regarding NK-cell-based immunotherapies. The central scientific question of this study pertains to the potential for boosting cytotoxicity in expanded and activated NK cells through the inhibition of inhibitory receptors. To address this question, we employed the CRISPR-Cas9 system to target three distinct inhibitory signaling pathways in NK cells. Specifically, we examined the roles of A2AR within the metabolic purinergic signaling pathway, CBLB as an intracellular regulator in NK cells, and the surface receptors NKG2A and CD96 in enhancing the antileukemic efficacy of NK cells. Following the successful expansion of NK cells, they were transfected with Cas9+sgRNA RNP to knockout A2AR, CBLB, NKG2A, and CD96. The analysis of indel frequencies for all four targets revealed good knockout efficiencies in expanded NK cells, resulting in diminished protein expression as confirmed by flow cytometry and Western blot analysis. Our in vitro killing assays demonstrated that NKG2A and CBLB knockout led to only a marginal improvement in the cytotoxicity of NK cells against AML and B-ALL cells. Furthermore, the antileukemic activity of CD96 knockout NK cells did not yield significant enhancements, and the blockade of A2AR did not result in significant improvement in killing efficiency. In conclusion, our findings suggest that CRISPR-Cas9-based knockout strategies for immune checkpoints might not be sufficient to efficiently boost the antileukemic functions of expanded (and activated) NK cells and, at the same time, point to the need for strong cellular activating signals, as this can be achieved, for example, via transgenic chimeric antigen receptor expression.
Changing the Electron Acceptor Specificity of Rhodobacter capsulatus Formate Dehydrogenase from NAD+ to NADP+
Authors: Kumar Hemant , Leimkühler Silke
Published: November 2023

Formate dehydrogenases catalyze the reversible oxidation of formate to carbon dioxide. These enzymes play an important role in CO2 reduction and serve as nicotinamide cofactor recycling enzymes. More recently, the CO2-reducing activity of formate dehydrogenases, especially metal-containing formate dehydrogenases, has been further explored for efficient atmospheric CO2 capture. Here, we investigate the nicotinamide binding site of formate dehydrogenase from Rhodobacter capsulatus for its specificity toward NAD+ vs. NADP+ reduction. Starting from the NAD+-specific wild-type RcFDH, key residues were exchanged to enable NADP+ binding on the basis of the NAD+-bound cryo-EM structure (PDB-ID: 6TG9). It has been observed that the lysine at position 157 (Lys157) in the β-subunit of the enzyme is essential for the binding of NAD+. RcFDH variants that had Glu259 exchanged for either a positively charged or uncharged amino acid had additional activity with NADP+. The FdsBL279R and FdsBK276A variants also showed activity with NADP+. Kinetic parameters for all the variants were determined and tested for activity in CO2 reduction. The variants were able to reduce CO2 using NADPH as an electron donor in a coupled assay with phosphite dehydrogenase (PTDH), which regenerates NADPH. This makes the enzyme suitable for applications where it can be coupled with other enzymes that use NADPH.
Ischemia-Reperfusion Increases TRPM7 Expression in Mouse Retinas
Authors: Martínez-Gil Natalia , Kutsyr Oksana , Fernández-Sánchez Laura , Sánchez-Sáez Xavier , Albertos-Arranz Henar , Sánchez-Castillo Carla , Vidal-Gil Lorena , Cuenca Nicolás , Lax Pedro , Maneu Victoria
Published: November 2023

Ischemia is the main cause of cell death in retinal diseases such as vascular occlusions, diabetic retinopathy, glaucoma, or retinopathy of prematurity. Although excitotoxicity is considered the primary mechanism of cell death during an ischemic event, antagonists of glutamatergic receptors have been unsuccessful in clinical trials with patients suffering ischemia or stroke. Our main purpose was to analyze if the transient receptor potential channel 7 (TRPM7) could contribute to retinal dysfunction in retinal pathologies associated with ischemia. By using an experimental model of acute retinal ischemia, we analyzed the changes in retinal function by electroretinography and the changes in retinal morphology by optical coherence tomography (OCT) and OCT-angiography (OCTA). Immunohistochemistry was performed to assess the pattern of TRPM7 and its expression level in the retina. Our results show that ischemia elicited a decrease in retinal responsiveness to light stimuli along with reactive gliosis and a significant increase in the expression of TRPM7 in Müller cells. TRPM7 could emerge as a new drug target to be explored in retinal pathologies associated with ischemia.
Multi-Omics Analysis of Circulating Exosomes in Adherent Long-Term Treated OSA Patients
Authors: Khalyfa Abdelnaby , Marin Jose M. , Sanz-Rubio David , Lyu Zhen , Joshi Trupti , Gozal David
Published: November 2023

Obstructive sleep apnea (OSA) is a highly prevalent chronic disease affecting nearly a billion people globally and increasing the risk of multi-organ morbidity and overall mortality. However, the mechanisms underlying such adverse outcomes remain incompletely delineated. Extracellular vesicles (exosomes) are secreted by most cells, are involved in both proximal and long-distance intercellular communication, and contribute toward homeostasis under physiological conditions. A multi-omics integrative assessment of plasma-derived exosomes from adult OSA patients prior to and after 1-year adherent CPAP treatment is lacking. We conducted multi-omic integrative assessments of plasma-derived exosomes from adult OSA patients prior to and following 1-year adherent CPAP treatment to identify potential specific disease candidates. Fasting morning plasma exosomes isolated from 12 adult patients with polysomnographically-diagnosed OSA were analyzed before and after 12 months of adherent CPAP therapy (mean ≥ 6 h/night) (OSAT). Exosomes were characterized by flow cytometry, transmission electron microscopy, and nanoparticle tracking analysis. Endothelial cell barrier integrity, wound healing, and tube formation were also performed. Multi-omics analysis for exosome cargos was integrated. Exosomes derived from OSAT improved endothelial permeability and dysfunction as well as significant improvement in tube formation compared with OSA. Multi-omic approaches for OSA circulating exosomes included lipidomic, proteomic, and small RNA (miRNAs) assessments. We found 30 differentially expressed proteins (DEPs), 72 lipids (DELs), and 13 miRNAs (DEMs). We found that the cholesterol metabolism (has04979) pathway is associated with lipid classes in OSA patients. Among the 12 subjects of OSA and OSAT, seven subjects had complete comprehensive exosome cargo information including lipids, proteins, and miRNAs. Multi-omic approaches identify potential signature biomarkers in plasma exosomes that are responsive to adherent OSA treatment. These differentially expressed molecules may also play a mechanistic role in OSA-induced morbidities and their reversibility. Our data suggest that a multi-omic integrative approach might be useful in understanding how exosomes function, their origin, and their potential clinical relevance, all of which merit future exploration in the context of relevant phenotypic variance. Developing an integrated molecular classification should lead to improved diagnostic classification, risk stratification, and patient management of OSA by assigning molecular disease-specific therapies.
Peripapillary Vascular Density in Childhood Glaucoma: A Pilot Comparative Study with Age and Sex Matched Healthy Subjects
Authors: Xydaki Flora , Arribas-Pardo Paula , Burgos-Blasco Barbara , Garcia-Feijoo Julian , Mendez-Hernandez Carmen
Published: November 2023

Purpose: The aim of this study concerns the evaluation of peripapillary vessel indices in childhood glaucoma (CG) and healthy subjects. Material and Methods: In this prospective, unicenter, observational cross-sectional study, patients with CG and age and sex-matched healthy subjects were included. We compared retinal nerve fiber layer (RNFL) measurements in optical coherence tomography (OCT), peripapillary vessel density (PVD), and the flux index (FI) of the superficial vascular plexus from OCT angiography (OCT-A) between CG patients and control groups. Results: We included 39 patients (68 eyes) with CG and 50 (95 eyes) healthy subjects. The peripapillary RNFL thickness, vessel density, and flux index were significantly lower in the CG group than in the control group. The mean PVD of CG patients was 0.52 ± 0.043%, compared with 0.55 ± 0.014%, p < 0.0001 in healthy subjects. The mean FI was 0.32 ± 0.054 versus 0.37 ± 0.028, p < 0.0001, in CG patients and healthy subjects, respectively. PVD and FI in the superior, inferior, and temporal sectors were significantly lower in CG. The peripapillary RNFL thickness showed a higher area under the ROC curve (AUROC) for discriminating healthy and CG eyes and was significantly different than the PVD (0.797, 95%CI 0.726–0.869; p < 0.0001 vs. 0.664, 95%CI 0.574–0.752; p 0.00037), p 0.012.Conclusions: PVD and FI show lower values in CG and correlate with RNFL thickness measurement but have lower diagnostic ability than RNFL thickness measurement. Our results reveal possible differences in the pathogenesis of microvascular compromise in childhood glaucoma patients.
Advancements in Understanding and Treating NAFLD: A Comprehensive Review of Metabolic-Associated Fatty Liver Disease and Emerging Therapies
Authors: Beiriger Jacob , Chauhan Kashyap , Khan Adnan , Shahzad Taha , Parra Natalia Salinas , Zhang Peter , Chen Sarah , Nguyen Anh , Yan Brian , Bruckbauer John , Halegoua-DeMarzio Dina
Published: November 2023

This paper provides a comprehensive review of the current understanding of non-alcoholic fatty liver disease (NAFLD) and its progression to non-alcoholic steatohepatitis (NASH), focusing on key factors influencing its pathogenesis and emerging therapeutic strategies. This review highlights the growing prevalence of NAFLD and NASH, emphasizing their multifactorial nature. The manuscript identifies various contributors to NAFLD development, including genetic, dietary, and environmental factors, while examining the intricate interplay between these factors and their impact on hepatic lipid metabolism, inflammation, and insulin resistance. Genetic predisposition, dietary fat intake, and excessive fructose consumption are discussed as significant contributors to NAFLD progression. The article emphasizes the lack of a single therapeutic approach and underscores the need for combination strategies. Lifestyle interventions, particularly weight loss through diet and exercise, remain crucial, while pharmacological options like GLP-1 receptor agonists, obeticholic acid, lanifibranor, and resmetirom show promise but require further validation. Bariatric surgery and emerging endoscopic procedures offer potential in eligible patients. In sum, this article underscores the complexity of NAFLD and NASH, addresses key factors influencing pathogenesis, and discusses emerging therapies advocating for a multifaceted approach to this increasingly prevalent and clinically relevant condition.
Time Series Prediction for Energy Consumption of Computer Numerical Control Axes Using Hybrid Machine Learning Models
Authors: Ströbel Robin , Probst Yannik , Deucker Samuel , Fleischer Jürgen
Published: November 2023

The prediction of energy-related time series for computer numerical control (CNC) machine tool axes is an essential enabler for the shift towards autonomous and intelligent production. In particular, a precise prediction of energy consumption is needed to determine the environmental impact of a product and the optimization of its production. For this purpose, a novel approach for predicting high-frequency time series of numerically controlled axes based on the program code to be executed is presented. The method involves simulative preprocessing of the input NC code to determine each axis’s acceleration, velocity, and process force. Combined with the material removal rate, these variables are input for a machine learning (ML) model that delivers axis-specific high-frequency time series predictions. Compared to common approaches, it is thus possible to make predictions for the variable energy consumption of machine tools for any tool path or target resolution in the time domain. Experiments show that this approach achieves a high precision when a robust learning data basis is available. For the X-, Y-, and Z-axis, errors of 0.2%, −1.09%, and 0.09% for aircut and of 0.15%, −3.55%, and 0.08% for material removal can be achieved. The potentials for further improvement are identified systematically.
In-Hospital Outcomes Following Surgical Revascularization of Chronic Total Coronary Occlusions
Authors: Fagu Albi , Berger Tim , Pingpoh Clarence , Kondov Stoyan , Kreibich Maximilian , Minners Jan , Czerny Martin , Siepe Matthias
Published: November 2023

Background and Objectives: Patients with chronic total occlusions of the coronary arteries are either treated with PCI or referred for surgical revascularization. We analyzed the patients with chronic occluded coronary arteries that were surgically treated and aimed to describe the anatomical characteristics, revascularization rates, and in-hospital outcomes achieved with coronary artery bypass grafting. Methods: Angiographic data of 2005 patients with coronary artery disease treated in our institution between January 2005 and December 2014 were retrospectively analyzed. A total of 1111 patients with at least one coronary total occlusion were identified. We reviewed the preoperative coronary angiograms and surgical protocols to determine the presence, localization, and revascularization of coronary occlusions. We also evaluated the perioperative data and in-hospital outcomes. Results: The median age of the study population was 68 years (25th–75th percentiles, 61.0–74.0). Three-vessel disease was present in 94.8% of patients and the rest (5.8%) had a two-vessel disease. The localizations of the occlusions were as follows: 68.4% in the RCA system, 26.4% in the LAD, and 28.5% in the LCX system. Multiple occlusions were present in 22.6% of the patients. Complete coronary total occlusion revascularization was achieved in 86.1% of the patients. The overall in-hospital mortality was 2.3%. The median in-hospital stay was 14.0 days. After logistic regression analysis, age (odds ratio 3.44 [95% confidence interval, 1.81–6.53], p < 0.001, for a 10-year increase) and the presence of peripheral artery disease (odds ratio 3.32 [1.39–7.93], p = 0.007) were the only statistically significant independent predictors of in-hospital mortality. Conclusions: A high revascularization rate and favorable in-hospital outcomes are achieved with coronary artery bypass surgery in patients with multi-vessel diseases and coronary total occlusions. Older age and the presence of peripheral artery disease are independent predictors of in-hospital mortality. A long-term follow-up and the type of graft (arterial vs. venous) used would bring more useful data for this type of revascularization.
Comparing the Effectiveness of Glucocorticoids in Preventing Hypertrophic Scar Diagnosis in Burn Patients
Authors: Joglar Alejandro , Song Juquan , Golovko George , Jay Jayson , Wolf Steven , El Ayadi Amina
Published: November 2023

Background and Objectives: The prevalence of hypertrophic scarring after a burn is approximately 70%. Despite advances in burn management, there is currently no gold standard treatment to reduce or prevent its occurrence. Glucocorticoids are frequently given to patients early after burns for other therapeutic purposes and have been shown to induce scar regression. Therefore, the purpose of the present work is to determine the incidence of hypertrophic scar diagnosis in burn patients who were administered glucocorticoid treatment using TriNetX, a large patient database. Materials and Methods: Patients diagnosed with hypertrophic scarring, hypertrophic disorders of the skin, or scar conditions and fibrosis of the skin after burn injury were identified in the TriNetX database. The glucocorticoids investigated include hydrocortisone, methylprednisolone, dexamethasone, triamcinolone, and prednisone. Patients were stratified into three groups based on total body surface area (TBSA) burned: 0–19%, 20–39%, and 40–100%. The risk ratio was evaluated for burn patients who received varying glucocorticoids after injury based on TBSA burned. Additionally, treatment pathways, time of treatment, and treatment purity pathways were evaluated. Results: In patients with a 0–19% TBSA burn, methylprednisolone showed a decreased risk of developing hypertrophic scar diagnosis. In those with a 20–39% TBSA burn or 40–100% TBSA burn, dexamethasone showed an increased risk of developing hypertrophic scar diagnosis. Additionally, dexamethasone was the most commonly administered glucocorticoid for burn patients and was most likely to be administered earlier after burn injury, comparatively. Conclusions: Methylprednisolone was associated with reduced hypertrophic scar diagnosis in burn patients independent of TBSA burn. While glucocorticoids are one of the mainstay treatments for hypertrophic scarring, further studies are needed to determine early therapeutic interventions that will reduce the potential for hypertrophic scar development in burn patients.
Multi-Phase Hydrothermal Fluid Events in the Giant Lannigou Gold Deposit, SW China: Insights from Calcite Sm–Nd Age, Trace Elements, and C-O-Sr Isotopes
Authors: Li Piyou , Hu Yuzhao , Tian Zhendong , Guan Shenjin , Fan Huijun
Published: November 2023

The Nanpanjiang basin hosts the world’s second-largest concentration of Carlin-type gold deposits. To decipher the origin and evolution of hydrothermal fluid, this study conducted Sm–Nd dating, in-situ trace element, and C-O-Sr isotopic analyses on three types of calcite samples from the giant Lannigou gold deposit in the Nanpanjiang basin, SW China. The type-I calcite, intergrown with Au-bearing arsenian pyrite, has an Sm–Nd isochron age of 213 ± 7 Ma (MSWD = 0.81), indicating that gold mineralization occurred in Late Triassic. The type-II calcite, which coexists with high-maturity bitumens and cut through the main-stage gold orebodies, yields an Sm–Nd age of 188 ± 14 Ma (MSWD = 0.34), representing a post-ore hydrocarbon accumulation event. The type-I and type-II calcite samples have low REE contents (5.28–51.6 ppm) and exhibit MREE-enriched and LREE-/HREE-depleted patterns. Combined with their identical C-O-Sr isotopes, we suggest that hydrothermal fluids responsible for the precipitation of type-I and type-II calcite samples were derived from a mixed metamorphic fluid and meteoric water source. In contrast, the type-III calcite samples, associated with realgar and orpiment, have distinct Mn, Sr, and As contents, REE patterns, and C-O-Sr isotopic composition from the type-I and II calcites, suggestive of different fluid sources. Based on our and previously published data, we propose that the fluid evolution, gold mineralization, and hydrocarbon accumulation in the Nanpanjiang basin are closely related to the Indosinian and Yanshanian orogenies in South China.
Archaeometric Analyses of dolia of the Final Bronze Age/Early Iron Age Found at Gagliato (CZ)–Calabria–Southern Italy
Authors: De Francesco Anna Maria , Tucci Antonina Maria , Guido Adriano , Rao Anna , Barca Donatella
Published: November 2023

In the hinterland of Soverato (in the Catanzaro province CZ) in Calabria, Italy, in the territory of Gagliato, on a series of terraced plains, the remains of an extensive settlement have been identified with archaeological evidence that includes the various phases of the protohistoric period and the Greek age. In the settlement, numerous protohistoric ceramic finds consisting in fragments of vascular shapes of various sizes and large dolia were recovered. This paper presents the preliminary results of the mineralogical, petrographic and paleontological analyses performed on dolia samples selected on the basis of the typology and the characteristics of the ceramic impasto. The dolia analyses were obtained through various analytical techniques. Petrographic and micropaleontological studies were performed using polarized optical microscopy (POM). The mineralogical analyses were carried out using X-ray diffractometry (XRD), both on selected ceramics and on the clayey and sandy sediments sampled for comparison around the settlement area. Furthermore, micromorphological observations have been performed, using scanning electron microscopy (SEM), on selected foraminiferal tests picked up from the clay sediments collected in the study area. Data highlight the remarkable compatibility between the mineralogical composition of the dolia and the sampled sediments, and they confirm that the raw materials for ceramic production may have occurred in an area not far from the settlement, as assumed by archaeologists.
A Conceptual Approach for the Design of New Catalysts for Ammonia Synthesis: A Metal—Support Interactions Review
Authors: Arroyo-Caire Javier , Diaz-Perez Manuel Antonio , Lara-Angulo Mayra Anabel , Serrano-Ruiz Juan Carlos
Published: November 2023

The growing interest in green ammonia production has spurred the development of new catalysts with the potential to carry out the Haber–Bosch process under mild pressure and temperature conditions. While there is a wide experimental background on new catalysts involving transition metals, supports and additives, the fundamentals behind ammonia synthesis performance on these catalysts remained partially unsolved. Here, we review the most important works developed to date and analyze the traditional catalysts for ammonia synthesis, as well as the influence of the electron transfer properties of the so-called 3rd-generation catalysts. Finally, the importance of metal–support interactions is highlighted as an effective pathway for the design of new materials with potential to carry out ammonia synthesis at low temperatures and pressures.
The Impact of Maternal Plant-Based Diet on Obstetric and Neonatal Outcomes—A Cross-Sectional Study
Authors: Przybysz Paulina , Kruszewski Adrian , Kacperczyk-Bartnik Joanna , Romejko-Wolniewicz Ewa
Published: November 2023

Background: In the last decades, plant-based diets have gained popularity. Pregnancy is not a contraindication to follow a meat-free diet. This study aimed to compare maternal and neonatal outcomes between women who followed a plant-based diet with those on an omnivore diet. Our second purpose was to investigate the association between physical activity level in combination with diet type and the occurrence of GDM and gestational hypertension. Methods: A questionnaire was distributed electronically via social media. The survey was conducted on a population of Polish women. Results: The final research group included 1015 women. The results showed that a maternal plant-based diet 6 months before pregnancy and during pregnancy does not change the incidence of GDM, anemia, and gestational hypertension. Moreover, no association was found between a diet type before conception and a delivery method or newborn birth weight. Among women who followed an omnivore diet, the risk of GDM was lower in a group with adequate physical activity during 6 months before conception (p = 0.0166). However, the combination of a plant-based diet with adequate activity during the preconception period did not influence GDM incidence. Conclusions: Our study indicates that a plant-based diet during the preconception period is not worse than an omnivore diet.
Relationship between Hydration Status and Muscle Catabolism in the Aged Population: A Cross-Sectional Study
Authors: Serra-Prat Mateu , Lorenzo Isabel , Martínez Jessica , Palomera Elisabet , Pleguezuelos Eulogio , Ferrer Pau
Published: November 2023

Background: The physiopathology of sarcopenia is still not completely understood. Aim: To assess the relationship between dehydration and skeletal muscle catabolism, muscle mass, and sarcopenia in an aged population. Methods: Observational cross-sectional study of community-dwelling subjects aged 70 years and older. Dehydration was assessed by plasma osmolarity; bioimpedance analysis (BIA) was used to assess body composition and water content; sarcopenia was established according to the EWGSOP-2 criteria; and 3-methyl-histidine (3MH) was used as an indicator of muscle catabolism. Results: 190 participants were recruited (77.4 years; 51.6% women). In total, 22.6% and 20.5% presented plasma osmolarity of 295–300 mOsm/L and >300 mOsm/L, respectively. Age was correlated with plasma osmolarity (rs = 0.439; p < 0.001). Plasma osmolarity was correlated with 3MH (rs = 0.360; p < 0.001) and showed an effect on 3MH levels, with an adjusted (by age, sex, and number of medications) beta of 0.283 (p < 0.001). BIA water content indicators showed no correlation with 3MH. Lower in sarcopenic compared to non-sarcopenic subjects were the intracellular water percentage (60.3 vs. 61.2%; p = 0.004) and intracellular water/free-fat mass ratio (44.3 vs. 45.0; p = 0.004). Conclusions: Dehydration is a highly prevalent clinical condition in aged populations, increases with age, and is associated with muscle catabolism but not sarcopenia.
Association between Children’s Difficulties, Parent-Child Sleep, Parental Control, and Children’s Screen Time: A Cross-Sectional Study in Japan
Authors: Arai Yusuke , Sasayama Daimei , Suzuki Kazuhiro , Nakamura Toshinori , Kuraishi Yuta , Washizuka Shinsuke
Published: November 2023

Children’s screen time may affect their growth and development. However, differences in the impact of various psychiatric and psychological factors on children’s screen time is a research gap. This study aimed to explore the differences in the influence of related factors affecting children’s screen time based on their sleep, difficulties, and parental control among Japanese elementary and junior high school students. A cross-sectional survey was conducted among parents in Japan. Data on screen time duration, parent–child background, strengths and difficulties, sleep variables, and parental control types were collected from 225 households. A regression analysis revealed that high Strengths and Difficulties Questionnaire (SDQ) scores (β = 0.166, p = 0.008), sleep duration (β = −0.281, p < 0.001), and parental control (β = −0.204, p = 0.001) were significantly related to children’s screen time. Additionally, it was found that parents’ late bedtimes affect children’s screen time by mediating children’s sleep duration. This study, together with previous research, provides comprehensive insights into design interventions to decrease the screen time of children in the Japanese context.
Co-Delivery of a High Dose of Amphotericin B and Itraconazole by Means of a Dry Powder Inhaler Formulation for the Treatment of Severe Fungal Pulmonary Infections
Authors: Celi Salomé S. , Fernández-García Raquel , Afonso-Urich Andreina I. , Ballesteros M. Paloma , Healy Anne Marie , Serrano Dolores R.
Published: November 2023

Over the past few decades, there has been a considerable rise in the incidence and prevalence of pulmonary fungal infections, creating a global health problem due to a lack of antifungal therapies specifically designed for pulmonary administration. Amphotericin B (AmB) and itraconazole (ITR) are two antifungal drugs with different mechanisms of action that have been widely employed in antimycotic therapy. In this work, microparticles containing a high dose of AmB and ITR (20, 30, and 40% total antifungal drug loading) were engineered for use in dry powder inhalers (DPIs) with an aim to improve the pharmacological effect, thereby enhancing the existing off-label choices for pulmonary administration. A Design of Experiment (DoE) approach was employed to prepare DPI formulations consisting of AmB-ITR encapsulated within γ-cyclodextrin (γ-CD) alongside functional excipients, such as mannitol and leucine. In vitro deposition indicated a favourable lung deposition pattern characterised by an upper ITR distribution (mass median aerodynamic diameter (MMAD) ~ 6 µm) along with a lower AmB deposition (MMAD ~ 3 µm). This offers significant advantages for treating fungal infections, not only in the lung parenchyma but also in the upper respiratory tract, considering that Aspergillus spp. can cause upper and lower airway disorders. The in vitro deposition profile of ITR and larger MMAD was related to the higher unencapsulated crystalline fraction of the drug, which may be altered using a higher concentration of γ-CD.
Synthesis and Thermoreversible Gelation of Coil–Rod Copolymers with a Dendritic Polyethylene Core and Multiple Helical Poly(γ-benzyl-L-glutamate) Arms
Authors: Lu Yuliang , Liu Dongtao , Wei Xinjie , Song Jiming , Xiao Qiaogang , Du Kezheng , Shi Xinbo , Gao Haiyang
Published: November 2023

Coil–rod copolymers with a dendritic polyethylene (DPE) core and multiple helical poly(γ-benzyl-L-glutamate) (PBLG) arms (DPE-(PBLG)n) were prepared by palladium-catalyzed copolymerization in tandem with ring-opening polymerization (ROP). Macroinitiator (DPE–(NH2)11) was firstly prepared by the group transformation of DPE–(OH)11 generated from palladium-catalyzed copolymerization of ethylene and acrylate comonomer. Coil–helical DPE-(PBLG)11 copolymers were prepared by ROP of γ-benzyl-L-glutamate-N-carboxyanhydride (BLG-NCA). These DPE-(PBLG)11 copolymers could form thermoreversible gels in toluene solvent, and the dendritic topology of the DPE core increased the critical gelation concentrations. The self-assembled nanostructure of gels was fully characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), small-angle X-ray scattering (SAXS), and wide-angle X-ray diffraction (WAXD), and the morphology of the fibrous structure was a twisted flat ribbon through a self-assembled nanoribbon mechanism. The self-assembled fibers formed by DPE-(PBLG45)11 are more heterogeneous and ramified than previously observed fibers formed by PBLG homopolymer and block copolymers.
The Impact of Liquid Components on Alteration of the Adhesion of Polyacrylate and Silicone Patches
Authors: Maciejewski Bartosz , Dłabiszewska Joanna , Mikolaszek Barbara , Sznitowska Małgorzata
Published: November 2023

Polyacrylates and polysiloxanes are polymers used in pressure-sensitive adhesive (PSA) patches. Liquid additives are co-solvents of the active substances or permeation enhancers, and their compatibility with the polymeric matrix and the effect on adhesive properties should be considered. The patches were prepared from commercial polyacrylates (three types of Duro-Tak®) and siloxanes (Bio-PSA® and Soft Skin Adhesive®). Propylene glycol, polyoxyethylene glycol, isopropyl myristate, triacetin, triethyl citrate and silicone oil were added (10% w/w). Formulations were evaluated microscopically and with a texture analyzer in terms of in vitro adhesiveness and hardness. Only silicone oil was compatible with the silicone matrices. The best compatibility of acrylic PSA was observed with triethyl citrate; one out of three Duro-Tak matrices was incompatible with every additive. In all compositions, the adhesiveness was impaired by the liquid additives. A significant drop in adhesiveness was noted after immersion of the patches in buffer and drying. The probe tack test was considered as the most useful for evaluation of the effect of the liquid additive on adhesiveness, but the results obtained with a spherical and cylindrical probe were contradictory. The structural changes caused by the additives were also demonstrated by a 90° peel test, considered as complementary to the tack test.
Injection Flow Rate Threshold Preventing Atypical In-Cavity Pressure during Low-Pressure Powder Injection Molding
Authors: Arès Francis , Delbergue Dorian , Demers Vincent
Published: November 2023

Controlling injection parameters is paramount when it comes to producing high-quality green parts using powder injection molding. This work combines experimental and numerical approaches to study the impact of injection parameters on mold in-cavity pressure and on the overall quality of green parts produced by low-pressure powder injection molding. The properties of two low-viscosity feedstocks (formulated from a water-atomized stainless-steel powder and wax-based binder system) were measured and implemented in an Autodesk Moldflow numerical model to quantify the molding pressures, which were finally validated using experimental real-scale injections. The results confirmed that an increase in mold temperature, an increase in feedstock temperature, and a decrease in solid loading decrease the mold in-cavity pressure, which was correlated with the feedstock viscosity. As a key result, real-scale injections confirmed that a minimum flow rate was required to avoid atypical high in-cavity pressure leading to several visual defects such as weld lines, flow marks, cracks, sinks, and incomplete filling. Due to differences in its thermal transfer properties, this flow rate threshold value decreases as the feedstock solid loading increases. For injection speeds higher than this value, the injection pressure measured experimentally was linearly correlated with the injection flow rate.
Seepage Simulation of Conglomerate Reservoir Based on Digital Core: A Case Study of the Baikouquan Formation in Mahu Sag, Junggar Basin
Authors: Zhang Daiyan , Hu Haisheng , Dong Yan , Wang Yingwei , Liu Dunqing , Liu Hongxian , Du Meng
Published: November 2023

Pore structure and flow characteristics are key factors affecting oil recovery rates in heterogeneous tight conglomerate reservoirs. Using micron computed tomography (CT) and modular automated processing system (MAPS) techniques, the pore structure of downhole core samples taken from Mahu’s tight conglomerate reservoirs was analyzed in detail, and a two-scale digital core pore network model with both a wide field of view and high resolution was constructed based on these pore structure data; the digital pore model was corrected according to the mercury intrusion pore size distribution date. Finally, we simulated flow characteristics within the digital model and compared the calculated permeability with the indoor permeability test date to verify the dependability of the pore network. The results indicated that the pore–throat of the conglomerate reservoir in Mahu was widely distributed and exhibited significant bimodal characteristics, with main throat channels ranging from 0.5 to 4 μm. The pore structure showed pronounced microscopic heterogeneity and intricate modalities, mainly consisting of dissolved pores, intergranular pores, and microfractures. These pores were primarily strip-like, isolated, and played a more crucial role in enhancing pore connectivity rather than contributing to the overall porosity. The matrix pores depicted by the MAPS were relatively smaller in size and more abundant in number, with no individual pore type forming a functional seepage channel. The permeability parameters obtained from the two-scale coarse-fine coupled pore network aligned with the laboratory experimental results, displaying an average coordination number of two. Flow simulation results indicated that the core’s microscopic pore structure affected the shape of the displacement leading edge, resulting in a tongue-in phenomenon during oil–water flow. The dominant flow channel was mainly dominated by water, while tongue-in and by-pass flow were the primary microscopic seepage mechanisms hindering oil recovery. These findings lay a foundation for characterizing and analyzing pore structure as well as investigating flow mechanisms in conglomerate reservoirs.
A Gridless DOA Estimation Method for Sparse Sensor Array
Authors: Gao Sizhe , Ma Hui , Liu Hongwei , Yang Junxiang , Yang Yang
Published: November 2023

Direction-of-arrival (DOA) estimation is still a pivotal research direction in array signal processing. Traditional algorithms based on the signal subspace and compressed sensing theory usually suffer from off-grid and computational complexity. Deep-learning-based methods usually face difficulty in obtaining labeled datasets. With the development of array technology, sparse sensor arrays can effectively reduce the number of sensors, which in turn reduces the complexity of the hardware. Therefore, effective DOA estimation algorithms for sparse sensor arrays need to be further investigated. An unsupervised deep learning method is proposed here to address the above issues. A training model was built based on the residual network structure. The DOA estimation was implemented using Vandermonde decomposition. Finally, the experimental findings confirmed the efficacy of the proposed algorithms presented in this article.
Low-Pass Image Filtering to Achieve Adversarial Robustness
Authors: Ziyadinov Vadim , Tereshonok Maxim
Published: November 2023

In this paper, we continue the research cycle on the properties of convolutional neural network-based image recognition systems and ways to improve noise immunity and robustness. Currently, a popular research area related to artificial neural networks is adversarial attacks. The adversarial attacks on the image are not highly perceptible to the human eye, and they also drastically reduce the neural network’s accuracy. Image perception by a machine is highly dependent on the propagation of high frequency distortions throughout the network. At the same time, a human efficiently ignores high-frequency distortions, perceiving the shape of objects as a whole. We propose a technique to reduce the influence of high-frequency noise on the CNNs. We show that low-pass image filtering can improve the image recognition accuracy in the presence of high-frequency distortions in particular, caused by adversarial attacks. This technique is resource efficient and easy to implement. The proposed technique makes it possible to measure up the logic of an artificial neural network to that of a human, for whom high-frequency distortions are not decisive in object recognition.
A Novel Tactile Sensing System Utilizing Magnetorheological Structures for Dynamic Contraction and Relaxation Motions
Authors: Park Yu-Jin , Kim Bo-Gyu , Lee Eun-Sang , Choi Seung-Bok
Published: November 2023

It is well known that the rheological properties of magnetorheological (MR) material change under a magnetic field. So far, most works on MR materials have been oriented toward actuating characteristics instead of sensing functions. In this work, to realize dynamic tactile motion, a spherical MR structure was designed as a sensor, incorporating a magnetic circuit core to provide maximum dynamic motion. After manufacturing a prototype (sample), a sinusoidal magnetic field of varying exciting frequency and magnitude was applied to the sample, and the dynamic contraction and relaxation motion depending on the exciting magnetic field was observed. Among the test results, when 10% deformation occurred, the instantaneous force generated was from 2.8 N to 8.8 N, and the force when relaxed was from 1.2 N to 3.5 N. It is also shown that the repulsive force within this range can be implemented using an acceptable input current. The special tactile sensing structure proposed in this work can be used as a sensor to measure the field-dependent viscoelastic properties of human tissues such as stomach, liver, and overall body. In addition, it could be usefully applied to robot surgery, because it can mimic the dynamic motions of various human organs under various surgical conditions.
Assessment of Forest Ecosystem Variations in the Lancang–Mekong Region by Remote Sensing from 2010 to 2020
Authors: Zhao Jing , Li Jing , Liu Qinhuo , Dong Yadong , Li Li , Zhang Hu
Published: November 2023

Five countries in the Lancang–Mekong region, including Myanmar, Laos, Thailand, Cambodia, and Vietnam, are facing the threat of deforestation, despite having a high level of forest coverage. Quantitatively assessing the forest ecosystem status and its variations based on remote sensing products for vegetation parameters is a crucial prerequisite for the ongoing phase of our future project. In this study, we analyzed forest health in the year 2020 using four vegetation indicators: forest coverage index (FCI), leaf area index (LAI), fraction of green vegetation cover (FVC), and gross primary productivity (GPP). Additionally, we introduced an ecosystem quality index (EQI) to assess the quality of forest health. To understand the long-term trends in the vegetation indicators and EQI, we also performed a linear regression analysis from 2010 to 2020. The results revealed that Laos ranked as the top-performing country for forest ecosystem status in the Lancang–Mekong region in 2020. However, the long-term trend analysis results showed that Cambodia experienced the most significant decline across all indicators, while Vietnam and Thailand demonstrated varying degrees of improvement. This study provides a quality assessment of forest health and its variations in the Lancang–Mekong region, which is crucial for implementing effective conservation strategies.
How Transformational Leaders Promote Employees’ Feedback-Seeking Behaviors: The Role of Intrinsic Motivation and Its Boundary Conditions
Authors: Cheng Jin , Li Kunlin , Cao Ting
Published: November 2023

Feedback-seeking behavior is critical to employee self-change and self-improvement, which is conducive to the sustainable development of employees’ own knowledge and abilities. Drawing on self-determination theory, this study aims to examine the underlying psychological mechanism and the boundary conditions of the relationship between transformational leadership and employees’ feedback-seeking behavior. Data were collected from a large manufacturing firm in China, encompassing 415 employees, and subsequently analyzed employing SPSS 22.0 and SPSS PROCESS Macro. The results showed that employee intrinsic motivation mediated the relationship between transformational leadership and employees’ feedback-seeking behavior. In addition, the positive association between transformational leadership and intrinsic motivation was more pronounced when organizational virtuousness was high than when it was low. On the contrary, the positive link between transformational leadership and intrinsic motivation was stronger when job complexity was low than when it was high. Organizational virtuousness and job complexity further moderated the indirect effect of transformational leadership on feedback-seeking behavior through intrinsic motivation. Our findings offer a better understanding of employee feedback-seeking behavior by considering factors that may trigger and influence employees’ psychological motivation. In this way, this study contributes to the literature on self-determination theory and feedback-seeking behavior.
Modeling Water Flow in Variably Saturated Porous Soils and Alluvial Sediments
Authors: Giudici Mauro
Published: November 2023

The sustainable exploitation of groundwater resources is a multifaceted and complex problem, which is controlled, among many other factors and processes, by water flow in porous soils and sediments. Modeling water flow in unsaturated, non-deformable porous media is commonly based on a partial differential equation, which translates the mass conservation principle into mathematical terms. Such an equation assumes that the variation of the volumetric water content (θ) in the medium is balanced by the net flux of water flow, i.e., the divergence of specific discharge, if source/sink terms are negligible. Specific discharge is in turn related to the matric potential (h), through the non-linear Darcy–Buckingham law. The resulting equation can be rewritten in different ways, in order to express it as a partial differential equation where a single physical quantity is considered to be a dependent variable. Namely, the most common instances are the Fokker–Planck Equation (for θ), and the Richards Equation (for h). The other two forms can be given for generalized matric flux potential (Φ) and for hydraulic conductivity (K). The latter two cases are shown to limit the non-linearity to multiplicative terms for an exponential K-to-h relationship. Different types of boundary conditions are examined for the four different formalisms. Moreover, remarks given on the physico-mathematical properties of the relationships between K, h, and θ could be useful for further theoretical and practical studies.
Utilization of Waste Straw Biomass in Suspension Magnetization Roasting of Refractory Iron Ore: Iron Recovery, Gas Analysis and Roasted Product Characterization
Authors: Cao Yue , Sun Yongsheng , Gao Peng , Li Wenbo
Published: November 2023

The straw-type biomass, as a green and alternative reductant for the suspension magnetization roasting (SMR) of iron ores, is proposed. The roasted products are investigated at a roasting temperature of 750 °C, the roasting time of 7.5 min and the biomass dose of 25%. The iron phase results indicate that hematite ores were reduced to magnetite by the biomass, and the magnetization transformation increased from 0.64 A·m2·g−1 to 36.93 A·m2·g−1. The iron ore microstructure evolutions of holes and fissures are detected by SEM-EDS. The biomass pyrolyzed to form CO2, CO, CH4, H2O, H2, C=O, benzene skeleton, C-Hand C-O compounds at 200–450 °C, while the mass loss of the magnetization roasting process occurred at 450–750 °C by using TG-FTIR. The GC/MS results showed that the organic gases preferred to produce the O-heterocycles at 329 °C while the hydrocarbons were dominant at the high temperature of 820 °C for the hematite ore and biomass mixture. The gas composition analysis explained that the reducing gaseous products (CO, CH4 and H2) were used as a reductant and consumed obviously by hematite ore in the SMR process. The innovative utilization of biomass waste was effective for iron recovery of hematite ore and contributes to the reduction of greenhouse gases and the protection of the environment.