bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Brand Ambassador dan Kualitas Produk Terhadap Keputusan Pembelian Skincare Whitelab (Studi Kasus Di PP. Al- Mahfudz Seblak Jombang, Jawa Timur)
Authors: Nurazizah Eva , Aji Tony Seno
Published: December 2023

Penelitian ini merupakan penelitian kuantitatif dengan metode survei. Populasi pada penelitian ini adalah konsumen Whitelab di Pesantren Putri Al Mahfudz. Pada pengambilan sebuah sampel berupa Purposive sampling memiliki jumlah sampel sebanyak 68 santri. Sehingga uji asumsi klasik terdiri dari uji normalitas, uji heteroskedastisitas, dan uji multikolinieritas dan analisis regresi linier berganda untuk menguji dan membuktikan hipotesis penelitian. Maka pada penelitian uji t menjelaskan bahwa variabel brand ambassador (X1) berpengaruh keasliannya terhadap sebuah keputusan pembelian (Y) serta variabel kualitas produk(X2) sangat berpengaruh keasliannya pada keputusan pembelian (Y). Maka hasil penelitian uji F (simultan) menunjukkan hasil bahwa brand ambassador (X1) dan kualitas produk (X2) simultan sangat bergantung kepada keasliannya yang terletak pada keputusan pembelian. (Y).
Pengaruh Reward dan Punishment Terhadap Kinerja Karyawan Di PT BPRS Bhakti Sumekar Sumenep
Authors: Zein Syahna , Frianto Agus
Published: December 2023

Penelitian ini merupakan penelitian kuantitatif dengan metode survei. Populasi pada penelitian ini adalah karyawan di PT Bank Pembiayaan Rakyat Syariah Bhakti Sumekar Sumenep. Teknik pengambilan sampel menggunakan sampel jenuh dengan jumlah sampel sebanyak 30 karyawan. Teknik pengumpulan data menggunakan kuesioner yang telah diuji validitas dan reliabilitasnya. Uji asumsi klasik meliputi uji normalitas, uji heteroskedastisitas, dan uji multikolinieritas dan analisis regresi linier berganda untuk menguji dan membuktikan hipotesis penelitian. Hasil penelitian uji t (parsial) menunjukkan bahwa variabel Reward (X1) memiliki pengaruh positif dan signifikan terhadap Kinerja karyawan (Y) dan variabel punishmen (X2) memiliki pengaruh yang tidak positif dan tidak signifikan terhadap kinerja karyawan (Y). Hasil penelitian uji F (simultan) menunjukkan hasil bahwa Reward (X1) dan punishment (X2) secara keseluruhan berpengaruh Positif dan signifikan terhadap kinerja karyawan (Y).
Criticidad epistémica de la praxis gerencial en la solución de problemas complejos
Authors:   Universidad Central de Venezuela, Olay Romero Raúl Patricio
Published: December 2023

La presente investigación está enfocada como una aproximación argumentativa y crítica al pensamiento epistémico, cuyas formas de razonar acompañan a la persona ante la posibilidad de conocer y comprender el mundo, fijar una posición en dicho contexto para buscar su verdad, obrar de acuerdo con la recta razón y generar mejor gerencia. Operando el discurso desde una metodología metarracionalista a partir de un abordaje caológico de construcciones dialécticas, creadas por el desenvolvimiento de lo que es necesario comprender, su reflexión de cierre ubica a la realidad y a la verdad en el campo de la gerencia como expresiones absolutamente subjetivas y relativas, cargadas de episteme y doxa, así como de intelectualismo y realismo volitivo-afectivo que pudieran resultar antinómicas, pero que en realidad resultan sinectivamente útiles para la construcción de saberes.
Entrevista com Sandrine Aeby Daghé
Authors: Stutz Lídia , Rodrigues Tognato Maria Izabel , Guedes Magalhães Tânia
Published: December 2023

Entrevista com Sandrine Aeby Daghé
Pandrug-resistant Acinetobacter baumannii from different clones and regions in Mexico have a similar plasmid carrying the blaOXA-72 gene
Authors: Fernández-Vázquez José Luis , Hernández-González Ismael Luis , Castillo-Ramírez Santiago , Jarillo-Quijada Ma Dolores , Gayosso-Vázquez Catalina , Mateo-Estrada Valeria Eréndira , Morfín-Otero Rayo , Rodríguez-Noriega Eduardo , Santos-Preciado José Ignacio , Alcántar-Curiel María Dolores
Published: December 2023

Background

Multidrug-resistant Acinetobacter baumannii is a common hospital-acquired pathogen. The increase in antibiotic resistance is commonly due to the acquisition of mobile genetic elements carrying antibiotic resistance genes. To comprehend this, we analyzed the resistome and virulome of Mexican A. baumannii multidrug-resistant isolates.

Methods

Six clinical strains of A. baumannii from three Mexican hospitals were sequenced using the Illumina platform, the genomes were assembled with SPAdes and annotated with Prokka. Plasmid SPAdes and MobRecon were used to identify the potential plasmid sequences. Sequence Type (ST) assignation under the MLST Oxford scheme was performed using the PubMLST database. Homologous gene search for known virulent factors was performed using the virulence factor database VFDB and an in silico prediction of the resistome was conducted via the ResFinder databases.

Results

The six strains studied belong to different STs and clonal complexes (CC): two strains were ST208 and one was ST369; these two STs belong to the same lineage CC92, which is part of the international clone (IC) 2. Another two strains were ST758 and one was ST1054, both STs belonging to the same lineage CC636, which is within IC5. The resistome analysis of the six strains identified between 7 to 14 antibiotic resistance genes to different families of drugs, including beta-lactams, aminoglycosides, fluoroquinolones and carbapenems. We detected between 1 to 4 plasmids per strain with sizes from 1,800 bp to 111,044 bp. Two strains from hospitals in Mexico City and Guadalajara had a plasmid each of 10,012 bp pAba78r and pAba79f, respectively, which contained the blaOXA-72 gene. The structure of this plasmid showed the same 13 genes in both strains, but 4 of them were inverted in one of the strains. Finally, the six strains contain 49 identical virulence genes related to immune response evasion, quorum-sensing, and secretion systems, among others.

Conclusion

Resistance to carbapenems due to pAba78r and pAba79f plasmids in Aba pandrug-resistant strains from different geographic areas of Mexico and different clones was detected. Our results provide further evidence that plasmids are highly relevant for the horizontal transfer of antibiotic resistance genes between different clones of A. baumannii.
Echocardiographic parameters indicating left atrial reverse remodeling after catheter ablation for atrial fibrillation
Authors: Angelini Eleonora , Sieweke Jan-Thorben , Berliner Dominik , Biber Saskia , Hohmann Stephan , Oldhafer Maximiliane , Schallhorn Sven , Duncker David , Veltmann Christian , Bauersachs Johann , Bavendiek Udo
Published: December 2023

Background

The echocardiographic parameters total atrial conduction time (PA-TDI duration), left atrial (LA) volume index (LAVI), and LA strain reflect adverse atrial remodeling and predict atrial fibrillation (AF).

Objectives

The aim of this study was to investigate echocardiographic parameters indicating reverse LA remodeling and potential associations with AF recurrence after pulmonary vein isolation (PVI).

Methods

This prospective observational study consecutively enrolled patients scheduled for PVI for symptomatic AF. Electrocardiogram (ECG) test and transthoracic echocardiography were performed the day before and after PVI and again 3 months later. AF recurrence was determined by Holter ECG at 3 months, and telephone follow-up at 12 months, after PVI. The parameters of LA remodeling [PA-TDI, LAVI, and LA strain analysis: reservoir strain (LASr), conduit strain (LAScd), contraction strain (LASct)] were determined by transthoracic echocardiography.

Results

A total of 48 patients were included in the study (mean age: 61.4 ± 12.2 years). PA-TDI significantly decreased the day after PVI compared with the baseline (septal PA-TDI 103 ± 13 vs. 82 ± 14.9 ms, p ≤ 0.001; lateral PA-TDI 122.4 ± 14.8 vs. 106.9 ± 14.4 ms, p ≤ 0.001) and at the 3-month follow-up (septal PA-TDI: 77.8 ± 14.5, p ≤ 0.001; lateral PA-TDI 105.2 ± 16.1, p ≤ 0.001). LAVI showed a significant reduction at the 3-month follow-up compared with the baseline (47.7 ± 14.4 vs. 40.5 ± 9.7, p < 0.05). LASr, LAScd, and LASct did not change after PVI compared with the baseline. AF recurred in 10 patients after PVI (21%). Septal PA-TDI, septal a', and LAVI/a' determined the day after PVI were associated with AF recurrence.

Conclusion

Changes in echocardiographic parameters of LA remodeling and function indicate that functional electromechanical recovery preceded morphological reverse remodeling of the left atrium after PVI. Furthermore, these changes in echocardiographic parameters indicating LA reverse remodeling after PVI may identify patients at high risk of AF recurrence.
Literature review on teachers’ mindsets, growth-oriented practices and why they matter
Authors: Laine Sonja , Tirri Kirsi
Published: December 2023

This literature review focuses on earlier research on teachers’ mindsets, i.e., teachers’ implicit beliefs about the malleability of basic human qualities such as intelligence. More specifically, the review focuses on what teachers’ mindsets are, how teachers’ mindsets manifest in the teaching, studying, and learning process, how teachers’ mindsets and growth-oriented practices are related to students’ mindsets and learning, and how teachers’ mindsets can be developed. Three electronic databases and backtracking references were used to search articles published between the years 2012 and 2023. After evaluating the eligibility of the articles, 64 were finally included in the review. The findings indicated that teachers’ mindsets and growth-oriented practices matter. Most earlier research has found some kind of connection between teachers’ mindsets and growth-oriented practices, and/or students’ mindsets and learning. The implications for teacher education, policy, and future research are discussed.
The combined effects of acidification and acute warming on the embryos of Pacific herring (Clupea pallasii)
Authors: Singh Nicole R. , Love Brooke , Murray Christopher S. , Sobocinski Kathryn L. , Cooper W. James
Published: December 2023

Anthropogenic climate change is projected to affect marine ecosystems by challenging the environmental tolerance of individuals. Marine fishes may be particularly vulnerable to emergent climate stressors during early life stages. Here we focus on embryos of Pacific herring (Clupea pallasii), an important forage fish species widely distributed across the North Pacific. Embryos were reared under a range of temperatures (10-16°C) crossed with two pCO2 levels (600 and 2000 μatm) to investigate effects on metabolism and survival. We further tested how elevated pCO2 affects critical thermal tolerance (CTmax) by challenging embryos to short-term temperature fluctuations. Experiments were repeated on embryos collected from winter and spring spawning populations to determine if spawning phenology corresponds with different limits of environmental tolerance in offspring. We found that embryos could withstand acute exposure to 20°C regardless of spawning population or incubation treatment, but that survival was greatly reduced after 2-3 hours at 25°C. We found that pCO2 had limited effects on CTmax. The survival of embryos reared under chronically warm conditions (12°, 14°, or 16°C) was significantly lower relative to 10°C treatments in both populations. Oxygen consumption rates (MO2) were also higher at elevated temperatures and pCO2 levels. However, heart contraction measurements made 48 hours after CTmax exposure revealed a greater increase in heart rate in embryos reared at 10°C compared to 16°C, suggesting acclimation at higher incubation temperatures. Our results indicate that Pacific herring are generally tolerant of pCO2 but are vulnerable to acute temperature stress. Importantly, spring-spawning embryos did not clearly exhibit a higher tolerance to heat stress compared to winter offspring.
Research on accessibility of port collection and distribution system from the perspective of carbon emissions
Authors: Wang Guangsheng , Song Yuheng , Yu Tong , Lei Zhiyi , Yang Yanhao , Huang Guangyu
Published: December 2023

Port accessibility is an important factor in the efficiency of a port collection and distribution systems. And the carbon emission of the collection and transportation system is large, which is an important factor that cannot be ignored when constructing the collection and transportation system. In order to analyze the carbon emission characteristics of the port collection and distribution system, the paper incorporates the carbon emission factor into the accessibility measurement of the port collection and distribution system. To solve the problem of unbalanced demand of each logistics node, the distribution of logistics demand in the system is realized by the method based on the appropriate freight volume. The carbon emission cost factor is introduced, and the accessibility measurement model based on the generalized cost impedance function is constructed. Taking the collection and distribution system of Douala Port in West Africa as an example to verify, the results show that, after adding the carbon emission factor, the accessibility of each logistics node shows different degrees of decline which shows that the addition of the carbon emission factor can be more comprehensive and can reflect the accessibility of the system.
Comparative performance evaluation of basalt fiber–modified hot in-place (HIP) recycling asphalt mixtures: site mixture versus lab mixture
Authors: Li Bo , Zhang Yu , Xiao Peng , Wang Yu , Kang Aihong , Zhang Yao
Published: December 2023

The performance improvement of hot in-place recycling asphalt mixtures has been a hot topic recently due to the widespread application of HIP recycling technology. Based on the maintenance project of the provincial pavement G233 Baoying section, basalt fibers were introduced into HIP recycling mixtures. The effect of basalt fiber on the comprehensive performance of recycled mixtures was investigated using high temperature stability tests, cracking resistance tests, water stability tests, and dynamic modulus tests. Moreover, the performance of site mixtures was comparatively investigated with that of lab-made mixtures to further explore the site mixing effect on the mixture performance. The results showed that the recycled mixtures without basalt fiber presented unqualified cracking resistance even though proper mixture design was performed. The addition of basalt fibers could greatly enhance the rutting resistance, low-temperature cracking resistance, and stripping resistance of HIP recycled mixtures by 105.2%, 102.3%, and 46.9%, respectively. Moreover, the mixing method also had a significant impact on the properties of mixtures. The recycled mixtures produced by the site re-mixing method showed inferior performance compared to that of mixtures produced by the lab mixing method. Specifically, the dynamic stability, low temperature failure strain, and stripping point values reduced by 44.1%, 16.2%, and 11.7%, respectively, indicating that the site re-mixing process was not as effective as the lab mixing process due to the weaker blending and mixing procedures of the site equipment. The results could be beneficial for the utilization of basalt fiber in HIP recycling technology.
Genomic characterization of Salmonella isolated from retail chicken and humans with diarrhea in Qingdao, China
Authors: Wang Wei , Cui Jing , Liu Feng , Hu Yujie , Li Fengqin , Zhou Zhemin , Deng Xiangyu , Dong Yinping , Li Shaoting , Xiao Jing
Published: December 2023

Salmonella, especially antimicrobial resistant strains, remains one of the leading causes of foodborne bacterial disease. Retail chicken is a major source of human salmonellosis. Here, we investigated the prevalence, antimicrobial resistance (AMR), and genomic characteristics of Salmonella in 88 out of 360 (24.4%) chilled chicken carcasses, together with 86 Salmonella from humans with diarrhea in Qingdao, China in 2020. The most common serotypes were Enteritidis and Typhimurium (including the serotype I 4,[5],12:i:-) among Salmonella from both chicken and humans. The sequence types were consistent with serotypes, with ST11, ST34 and ST19 the most dominantly identified. Resistance to nalidixic acid, ampicillin, tetracycline and chloramphenicol were the top four detected in Salmonella from both chicken and human sources. High multi-drug resistance (MDR) and resistance to third-generation cephalosporins resistance were found in Salmonella from chicken (53.4%) and humans (75.6%). In total, 149 of 174 (85.6%) Salmonella isolates could be categorized into 60 known SNP clusters, with 8 SNP clusters detected in both sources. Furthermore, high prevalence of plasmid replicons and prophages were observed among the studied isolates. A total of 79 antimicrobial resistant genes (ARGs) were found, with aac(6′)-Iaa, blaTEM-1B, tet(A), aph(6)-Id, aph(3″)-Ib, sul2, floR and qnrS1 being the dominant ARGs. Moreover, nine CTX-M-type ESBL genes and the genes blaNMD-1, mcr-1.1, and mcr-9.1 were detected. The high incidence of MDR Salmonella, especially possessing lots of mobile genetic elements (MGEs) in this study posed a severe risk to food safety and public health, highlighting the importance of improving food hygiene measures to reduce the contamination and transmission of this bacterium. Overall, it is essential to continue monitoring the Salmonella serotypes, implement the necessary prevention and strategic control plans, and conduct an epidemiological surveillance system based on whole-genome sequencing.
Transcriptional changes during isoproterenol-induced cardiac fibrosis in mice
Authors: Nanda Disha , Pant Priyanka , Machha Pratheusa , Sowpati Divya Tej , Kumarswamy Regalla
Published: December 2023

Introduction: β-adrenergic stimulation using β-agonists such as isoproterenol has been routinely used to induce cardiac fibrosis in experimental animal models. Although transcriptome changes in surgical models of cardiac fibrosis such as transverse aortic constriction (TAC) and coronary artery ligation (CAL) are well-studied, transcriptional changes during isoproterenol-induced cardiac fibrosis are not well-explored.

Methods: Cardiac fibrosis was induced in male C57BL6 mice by administration of isoproterenol for 4, 8, or 11 days at 50 mg/kg/day dose. Temporal changes in gene expression were studied by RNA sequencing.

Results and discussion: We observed a significant alteration in the transcriptome profile across the different experimental groups compared to the saline group. Isoproterenol treatment caused upregulation of genes associated with ECM organization, cell–cell contact, three-dimensional structure, and cell growth, while genes associated with fatty acid oxidation, sarcoplasmic reticulum calcium ion transport, and cardiac muscle contraction are downregulated. A number of known long non-coding RNAs (lncRNAs) and putative novel lncRNAs exhibited differential regulation. In conclusion, our study shows that isoproterenol administration leads to the dysregulation of genes relevant to ECM deposition and cardiac contraction, and serves as an excellent alternate model to the surgical models of heart failure.
P2X7 receptor activation awakes a dormant stem cell niche in the adult spinal cord
Authors: Falco María Victoria , Fabbiani Gabriela , Maciel Cecilia , Valdivia Spring , Vitureira Nathalia , Russo Raúl E.
Published: December 2023

The ependyma of the spinal cord is a latent stem cell niche that is reactivated by injury, generating new cells that migrate to the lesion site to limit the damage. The mechanisms by which ependymal cells are reactivated after injury remain poorly understood. ATP has been proposed to act as a diffusible “danger signal” to alert about damage and start repair. Indeed, spinal cord injury (SCI) generates an increase in extracellular ATP around the lesion epicenter that lasts for several hours and affects the functional outcome after the damage. The P2X7 receptor (P2X7r) has functional properties (e.g., low sensitivity for ATP, high permeability for Ca2+) that makes it a suitable candidate to act as a detector of tissue damage. Because ependymal cells express functional P2X7r that generate an inward current and regenerative Ca2+ waves, we hypothesize that the P2X7r has a main role in the mechanisms by which progenitor-like cells in the ependyma react to tissue damage. To test this possibility, we simulated the P2X7r activation that occurs after SCI by in vivo intraspinal injection of the selective agonist BzATP nearby the central canal. We found that BzATP rescued ependymal cells from quiescence by triggering a proliferative response similar to that generated by injury. In addition, P2X7r activation by BzATP induced a shift of ependymal cells to a glial fibrillary acidic protein (GFAP) phenotype similar to that induced by injury. However, P2X7r activation did not trigger the migration of ependyma-derived cells as occurs after tissue damage. Injection of BzATP induced the expression of connexin 26 (Cx26) in ependymal cells, an event needed for the proliferative reaction after injury. BzATP did not induce these changes in ependymal cells of P2X7–/– mice supporting a specific action on P2X7r. In vivo blockade of P2X7r with the potent antagonist AZ10606120 reduced significantly the injury-induced proliferation of ependymal cells. Our data indicate that P2X7r has a key role in the “awakening” of the ependymal stem cell niche after injury and suggest purinergic signaling is an interesting target to improve the contribution of endogenous progenitors to repair.
The effect on blink frequency of a selective blue-filtering photochromic lens in patients with benign essential blepharospasm
Authors: Monschein Tobias , Zrzavy Tobias , Weber Corinna , Kuzmina Zoya , Gutstein Walter , Sycha Thomas
Published: December 2023

Objective

The aim of this clinical study was to assess the impact of a non-invasive selective blue-filtering photochromic lens coating Crizal Prevencia on the treatment and response of benign essential blepharospasm (BEB).

Patients and methods

Twenty-four patients were recruited in the outpatient clinic of the Medical University of Vienna in a randomized, double-blind, cross-over study design. Blink frequencies were assessed in patients with BEB before and 14 days after intervention with either a filtering ophthalmic lens or a placebo lens, respectively. Outcome parameters include sub-group analysis of a blink frequency under six different conditions: three photopic conditions, one resting condition, one reading condition, and one video game condition.

Results

From 24 recruited patients, 15 patients were available for final analysis. Comparing the optical blue filtering lens to placebo, showed a reduced blink frequency in specific subtests, but not compared to baseline.

Discussion

In conclusion, optical filtering glasses might have a beneficial effect on BEB and provide a non-invasive therapeutic add-on option, in addition to botulinum neurotoxin therapy, for patients with BEB and should necessarily be further investigated in a multicenter setting, resulting in larger sample sizes to gain valid information about the effect of photochromic blue filter glasses in BEB.

Clinical trial registration: https://drks.de/search/en/trial/DRKS00032135, DRKS00032135.
Evaluating the efficacy of purchased antisense oligonucleotides to reduce mouse and human tau in vivo
Authors: Vemula Pranav , Schoch Kathleen M. , Miller Timothy M.
Published: December 2023

Many preclinical and clinical studies support the use of antisense oligonucleotides (ASOs) as effective therapeutic strategies. However, acquiring ASOs for research purposes may be limited by partnerships with the pharmaceutical companies. Our lab previously developed an effective ASO strategy to lower human tau and reverse pathology in aged tauopathy model mice. Testing the efficacy of purchased tau lowering ASOs would provide support for these reagents as broad research tools. Purchased mouse and human tau lowering ASOs were infused or injected intracerebroventricularly into wildtype and tau transgenic mice. Following treatment, brain tissue evaluated for ASO distribution and levels of tau mRNA, protein, and phosphorylated tau. We show that purchased ASOs enter cell types of the brain and effectively decrease mouse or human tau mRNA and protein levels. Human tau lowering ASO treatment in PS19 mice decreased phosphorylated tau and gliosis relative to saline-treated PS19 mice, consistent with our previous study using a non-commercial tau lowering ASO. The results of this study demonstrate the efficacy of purchased tau targeting ASOs in vivo to support their broad use by researchers.
High-intensity exercise increases breast milk adiponectin concentrations: a randomised cross-over study
Authors: Holmen Mads , Giskeødegård Guro F. , Moholdt Trine
Published: December 2023

Introduction

Adiponectin plays a role in glucose and fat metabolism and is present in human breast milk. It has been postulated that higher breast milk adiponectin concentrations may prevent rapid weight gain in infancy. Prior research indicates that circulating adiponectin increases acutely after endurance exercise, but no prior research has investigated the effect of exercise on breast milk adiponectin concentrations. The purpose of this randomised, cross-over study was to determine the acute effects of endurance exercise on adiponectin concentrations in human breast milk.

Methods

Participants who were exclusively breastfeeding a 6–12 week-old term infant (N = 20) completed three conditions in the laboratory: (1) Moderate-intensity continuous training (MICT), (2) High-intensity interval training (HIIT), and (3) No activity (REST). At each condition, we collected breast milk at 07:00 h (before exercise/rest), 11:00 h (immediately after exercise/rest), 12:00 h (1 h after exercise/rest), and 15:00 h (4 h after exercise/rest) and determined adiponectin concentrations using enzyme-linked immunosorbent assay. We compared changes in adiponectin concentrations after MICT and HIIT, adjusted for the morning concentration on each test day, with those after REST, using paired t-tests.

Results

Adiponectin concentrations increased 1 h after HIIT, from 4.6 (± 2.2) μg/L in the 07:00 h sample to 5.6 (± 2.6) μg/L. This change was 0.9 μg/L (95% confidence interval 0.3 to 1.5) greater than the change between these two timepoints in the REST condition (p = 0.025). There were no other statistically significant changes in adiponectin concentrations.

Conclusion

HIIT may increase adiponectin concentrations in breast milk acutely after exercise. Further studies should determine the impact of exercise-induced elevations in breast milk adiponectin concentrations on growth and metabolism in infancy.
The roles of serum vitamin D and tobacco smoke exposure in insomnia: a cross-sectional study of adults in the United States
Authors: Gao Tianci , Hou Mengxing , Wang Qianfei , Liu Dong , Chen Fenqiao , Xing Yueyi , Mei Jianqiang
Published: December 2023

Aim

Tobacco smoke exposure and vitamin D (VD) status were both associated with insomnia. However, the combined effect of smoking and VD on insomnia has not been discussed. This study aimed to explore the role of VD in the association between tobacco smoke exposure and insomnia.

Methods

Data on adults were extracted from the National Health and Nutrition Examination Surveys (NHANES) database in 2005–2008 for this cross-sectional study. Weighted univariate and multivariate logistic regression analyses were used to explore the associations between serum cotinine, serum VD, and insomnia. A surface diagram was drawn to reflect the effect of VD on the association between serum cotinine and insomnia. In addition, the potential regulating effect of VD in subgroups of smoking status was also performed. The evaluation index was odds ratios (ORs) with 95% confidence intervals (CIs).

Results

Among the eligible participants, 1,766 had insomnia. After adjusting for covariates, we found that elevated serum cotinine levels were associated with higher odds of insomnia [OR = 1.55, 95% CI: (1.22, 1.97)]. However, the relationship between serum VD level and insomnia was not significant (P = 0.553). Higher serum cotinine levels were also associated with higher odds of insomnia [OR = 1.52, 95% CI: (1.17, 1.98)] when serum VD level was <75 nmol/L; however, this relationship became non-significant when serum VD concentration was elevated (P = 0.088). Additionally, the potential regulating effect of VD was also found in adults who were not smoking.

Conclusion

VD may play a potential regulative role in the association between tobacco smoke exposure and insomnia. Further studies are needed to clarify the causal relationships between VD, tobacco smoke exposure, and insomnia.
Influence of parotid lymph node metastasis on distant metastasis in parotid gland cancer
Authors: Meng Fan , Yuan Junhui , Zhang Xu , Liu Jun , Li Hailiang
Published: December 2023

Background

The aim of this study was to analyze the impact of the number and extranodal extension (ENE) of positive parotid lymph nodes (LNs) on distant metastasis in parotid cancer.

Methods

Patients with surgically treated parotid cancer were retrospectively enrolled. The hazard ratios (HRs) of the number and ENE of positive parotid LNs on distant metastasis-free survival (DMFS) were evaluated.

Results

In the Cox model, the groups with zero and one positive LN had comparable 10-year DMFS, but those with two positive LNs had an HR of 2.11 (95% CI: 1.36–5.29), and those with three or more positive LNs had an HR of 3.31 (95% CI: 2.05–8.43). The presence of ENE in parotid LNs did not impact the DMFS (p = 0.462; HR: 2.17; 95% CI: 0.84–6.17).

Conclusion

Parotid LN metastasis was associated with decreased DMFS; this effect was mainly driven by the number of positive LNs rather than ENE.
Current status and prospect of PET-related imaging radiomics in lung cancer
Authors: Tang Xin , Wu Fan , Chen Xiaofen , Ye Shengli , Ding Zhongxiang
Published: December 2023

Lung cancer is highly aggressive, which has a high mortality rate. Major types encompass lung adenocarcinoma, lung squamous cell carcinoma, lung adenosquamous carcinoma, small cell carcinoma, and large cell carcinoma. Lung adenocarcinoma and lung squamous cell carcinoma together account for more than 80% of cases. Diverse subtypes demand distinct treatment approaches. The application of precision medicine necessitates prompt and accurate evaluation of treatment effectiveness, contributing to the improvement of treatment strategies and outcomes. Medical imaging is crucial in the diagnosis and management of lung cancer, with techniques such as fluoroscopy, computed radiography (CR), digital radiography (DR), computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET)/CT, and PET/MRI being essential tools. The surge of radiomics in recent times offers fresh promise for cancer diagnosis and treatment. In particular, PET/CT and PET/MRI radiomics, extensively studied in lung cancer research, have made advancements in diagnosing the disease, evaluating metastasis, predicting molecular subtypes, and forecasting patient prognosis. While conventional imaging methods continue to play a primary role in diagnosis and assessment, PET/CT and PET/MRI radiomics simultaneously provide detailed morphological and functional information. This has significant clinical potential value, offering advantages for lung cancer diagnosis and treatment. Hence, this manuscript provides a review of the latest developments in PET-related radiomics for lung cancer.
Acute gastroenteritis—changes to the recommended original oral rehydrating salts: a review
Authors: Lifschitz Carlos , Kozhevnikov Oleksii , Oesterling Christine , Anbar Amira , Walker Steven
Published: December 2023

The World Health Organization recommended a formulation of oral rehydration salts as the intervention of choice for the treatment of acute gastroenteritis. While of value for the replacement of fluids and electrolytes, the formulation does not reduce stool volume, frequency, or symptom duration. This may prevent wide acceptance. To increase tolerability, shorten the duration of diarrhea and improve parental quality of life, several modifications to the original formula have been proposed. These include; low osmolarity, rice-based, glucose polymers as an alternative to glucose, the addition of probiotics, prebiotics and/or zinc, and various other ingredients. Here we summarize evidence regarding such changes and additions.
Aggressive approach for spontaneous pneumothorax treatment in children with Marfan syndrome?
Authors: Zarfati Angelo , Frediani Simone , Pardi Valerio , Aloi Ivan Pietro , Madafferi Silvia , Accinni Antonella , Bertocchini Arianna , Inserra Alessandro
Published: December 2023

Background and objectives

Marfan syndrome (MS) is a systemic disease of connective tissues consisting of a variable combination of anomalies. These patients have an increased risk of spontaneous pneumothorax (SP). However, there is a scarcity of pediatric literature on management, and no specific guidelines exist. Our aim was to analyze the management of spontaneous pneumothorax in children and adolescents with Marfan syndrome, comparing syndromic and non-syndromic patients.

Methods

Retrospective analysis of pediatric patients (18 years) with SP diagnosed at our tertiary pediatric hospital (January 10–June 22), with special emphasis on diagnosis, treatment, and follow-up (FU).

Results

Sixty-six patients with SP were identified, with nine (13%) having MS. In terms of baseline, there were no significant differences between the groups (age, sex, asthma, symptoms, and side, first-line treatment and hospitalization length). Overall, Marfan patients had significantly more first-line treatment failures requiring additional surgery, as well as more contralateral occurrences and the need for surgery/chest drain during the follow-up. Instead, conservative management resulted in significantly more ipsilateral recurrences and the need for surgery/chest drain in Marfan patients than controls during the follow-up.

Conclusions

Treatment failure, contralateral occurrence, ipsilateral recurrence, and the need for surgery/chest drain during follow-up make management of patients with Marfan syndrome and spontaneous pneumothorax more difficult. In patients with a diagnosed MS a more aggressive first-line management should be considered, bearing in mind the higher risks of this population.
The role of plant proteases in the response of plants to abiotic stress factors
Authors: Luciński Robert , Adamiec Małgorzata
Published: December 2023

The primary function of proteolytic enzymes is the hydrolysis of peptide bonds. Enzymes responsible for catalyzing this reaction are commonly referred to as proteases. The vast majority of these enzymes belong to the class of hydrolases and operate in aqueous environments. However, there is a distinct group of proteases known as intramembrane proteases, which are integral membrane proteins capable of cleaving peptide bonds in the hydrophobic environment of biological membranes. Proteolysis serves several essential functions in plant cells, ranging from the degradation of damaged and unnecessary proteins to the removal of non-functional protein aggregates. It plays a role in the quality control system for proteins and even releases transcription factors from membrane proteins. Proteolytic processes are indispensable at every stage of plant development and allow for the modification of the cell’s protein composition based on developmental needs and environmental requirements. Proteases are also enzymatic components of the cell that facilitate the efficient regeneration of many key metabolic pathways, such as photosynthesis and respiration. The response of plant cells and the entire plant organism to various biotic and abiotic stresses often requires the remodeling of metabolic pathways, the regeneration of key enzymatic complexes, or changes in the protein profile. By participating in all these processes, proteases constitute a crucial element of the cellular response to environmental stresses. The aim of this work is to review the role that individual proteases play in the response of plant cells to abiotic stress factors, such as drought, salinity, cold, temperature, and light.
Plasma phospholipids profiling changes were associated with the therapeutic response to Roxadustat in peritoneal dialysis patients
Authors: Yang Ya-Hui , Saimaiti Yishakejiang , Zhao Yang , Tang Wen
Published: December 2023

Background: Elevated Phospholipids (PLs) and sphingolipid (SM) metabolism relates to with poor clinical status and adverse outcome of end-stage kidney disease patients undergoing peritoneal dialysis (PD). Studies have suggested that the use of hypoxia-inducible factor prolyl hydroxylase inhibitor (HIF-PHI) (Roxadustat) is associated with altered lipid metabolism. Observing on how PLs and SMs changes after the HIF-PHI treatment in PD patients may help understand the possible effect of HIF-PHI on PD patients besides correcting of anemia.

Materials and methods: Stable peritoneal dialysis (PD) patients treated with Roxadustat for over 3 months were included. Phospholipid and sphingolipid metabolism were measured before and after treatment.

Results: 25 PD patients were included. Overall, phospholipid and sphingolipid metabolism showed a decreasing trend after HIF-PHI treatment. Levels of LysoPC (20:0), 1,2-dilinoleoyl-sn-glycero-3-phosphocholine [CisPC (DLPC) (18:2)], lysophosphatidylethanolamine (LysoPE) (14:0), and sphingomyelin (d18:1/17:0) (17:0) were significantly decreased (all p < 0.05). Further regression analyses confirmed the significant relationship between the increased of hemoglobin levels and the decrease in egg lyso PC: phosphatidylethanolamines (PE) (16:0–18:1), PE (16:0–18:2), PE (16:0–22:6), PE (18:0–20:4), PE (18:0–18:2), LysoPE (18:0), LysoPE (18:1), and phosphatidylcholine (PC) (18:1–18:0).

Conclusion: This study demonstrated that phospholipid and sphingolipid metabolism decreased after administration of HIF-PHI and was associated with improvement of anemia.
Prediction of vertical distribution of SPAD values within maize canopy based on unmanned aerial vehicles multispectral imagery
Authors: Chen Bo , Huang Guanmin , Lu Xianju , Gu Shenghao , Wen Weiliang , Wang Guangtao , Chang Wushuai , Guo Xinyu , Zhao Chunjiang
Published: December 2023

Real-time monitoring of canopy chlorophyll content is significant in understanding crop growth status and guiding precision agricultural management. Remote sensing methods have demonstrated great potential in this regard. However, the spatiotemporal heterogeneity of chlorophyll content within crop canopies poses challenges to the accuracy and stability of remote sensing estimation models. Hence, this study aimed to develop a novel method for estimating canopy chlorophyll content (represented by SPAD values) in maize (Zea mays L.) canopies. Firstly, we investigated the spatiotemporal distribution patterns of maize canopy SPAD values under varying nitrogen application rates and different growth stages. The results revealed a non-uniform, “bell-shaped” curve distribution of maize canopy SPAD values in the vertical direction. Nitrogen application significantly influenced the distribution structure of SPAD values within the canopy. Secondly, we achieved satisfactory results by fitting the Lorentz peak distribution function to the SPAD values of different leaf positions in maize. The fitting performance, evaluated using R2 and RMSE, ranged from 0.69 to 0.98 and 0.45 to 3.59, respectively, for the year 2021, and from 0.69 to 0.77 and 2.38 to 6.51, respectively, for the year 2022.Finally, based on the correlation between canopy SPAD values and vegetation indices (VIs) at different growth stages, we identified the sensitive leaf positions for the selected CCCI (Canopy Chlorophyll Index) in each growth stage. The 6th (r = 0.662), 4th (r = 0.816), 12th (r = 0.722), and 12th (r = 0.874) leaf positions exhibited the highest correlations. Compared to the estimation model using canopy wide SPAD values, the model based on sensitive leaf positions showed improved accuracy, with increases of 34%, 3%, 20%, and 3% for each growth stage, respectively. In conclusion, the findings of this study contribute to the enhancement of chlorophyll content estimation models in crop canopies and provide valuable insights for the integration of crop growth models with remote sensing methods.
DNA barcoding and comparative RNA-Seq analysis provide new insights into leaf formation using a novel resource of high-yielding Epimedium koreanum
Authors: Yang Jiaxin , Fan Siqing , Guo Min , Xie Zhaoqi , Cheng Qiqing , Gao Puxin , Cheng Chunsong
Published: December 2023

Epimedium koreanum Nakai, a well-known traditional Chinese medicinal herb, has been widely used to treat osteoporosis and sexual dysfunction for thousands of years. However, due to the decreasing population of East Asian natural resources, yearly output of Epimedium crude herb has been in low supply year by year. In this study, an unusual variety of E. koreanum was discovered in Dunhua, Jilin Province, the northernmost area where this variety was found containing 6 individuals, with three branches that had 27 leaflets, which is much more than the typical leaflet number of 9. Firstly, the novel E. koreanum varety was identified using DNA barcodes. Then, 1171 differentially expressed genes (DEGs) were discovered through parallel RNA-seq analysis between the newly discovered variety and wild type (WT) E. koreanum plant. Furthermore, the results of bioinformatics investigation revealed that 914 positively and 619 negatively correlated genes associated with the number of leaflets. Additionally, based on RNA-Seq and qRT-PCR analysis, two homologous hub TCP genes, which were commonly implicated in plant leaf development, and shown to be up regulated and down regulated in the discovered newly variety, respectively. Thus, our study discovered a novel wild resource for leaf yield rewarding medicinal Epimedium plant breeding, provided insights into the relationship between plant compound leaf formation and gene expression of TCPs transcription factors and other gene candidates, providing bases for creating high yield cultivated Epimedium variety by using further molecular selection and breeding techniques in the future.
Structural analysis and molecular substrate recognition properties of Arabidopsis thaliana ornithine transcarbamylase, the molecular target of phaseolotoxin produced by Pseudomonas syringae
Authors: Nielipinski Maciej , Pietrzyk-Brzezinska Agnieszka J. , Wlodawer Alexander , Sekula Bartosz
Published: December 2023

Halo blight is a plant disease that leads to a significant decrease in the yield of common bean crops and kiwi fruits. The infection is caused by Pseudomonas syringae pathovars that produce phaseolotoxin, an antimetabolite which targets arginine metabolism, particularly by inhibition of ornithine transcarbamylase (OTC). OTC is responsible for production of citrulline from ornithine and carbamoyl phosphate. Here we present the first crystal structures of the plant OTC from Arabidopsis thaliana (AtOTC). Structural analysis of AtOTC complexed with ornithine and carbamoyl phosphate reveals that OTC undergoes a significant structural transition when ornithine enters the active site, from the opened to the closed state. In this study we discuss the mode of OTC inhibition by phaseolotoxin, which seems to be able to act only on the fully opened active site. Once the toxin is proteolytically cleaved, it mimics the reaction transition state analogue to fit inside the fully closed active site of OTC. Additionally, we indicate the differences around the gate loop region which rationally explain the resistance of some bacterial OTCs to phaseolotoxin.
Lipid modulation contributes to heat stress adaptation in peanut
Authors: Spivey William W. , Rustgi Sachin , Welti Ruth , Roth Mary R. , Burow Mark D. , Bridges William C. , Narayanan Sruthi
Published: December 2023

At the cellular level, membrane damage is a fundamental cause of yield loss at high temperatures (HT). We report our investigations on a subset of a peanut (Arachis hypogaea) recombinant inbred line population, demonstrating that the membrane lipid remodeling occurring at HT is consistent with homeoviscous adaptation to maintain membrane fluidity. A major alteration in the leaf lipidome at HT was the reduction in the unsaturation levels, primarily through reductions of 18:3 fatty acid chains, of the plastidic and extra-plastidic diacyl membrane lipids. In contrast, levels of 18:3-containing triacylglycerols (TGs) increased at HT, consistent with a role for TGs in sequestering fatty acids when membrane lipids undergo remodeling during plant stress. Polyunsaturated acyl chains from membrane diacyl lipids were also sequestered as sterol esters (SEs). The removal of 18:3 chains from the membrane lipids decreased the availability of susceptible molecules for oxidation, thereby minimizing oxidative damage in membranes. Our results suggest that transferring 18:3 chains from membrane diacyl lipids to TGs and SEs is a key feature of lipid remodeling for HT adaptation in peanut. Finally, QTL-seq allowed the identification of a genomic region associated with heat-adaptive lipid remodeling, which would be useful for identifying molecular markers for heat tolerance.
Psychiatric trainees as second victims after exposure to patient suicide: a French qualitative study
Authors: Durif-Bruckert Christine , Cuvillier Bruno , Vieux Maxime , Chalancon Benoit , Villeval Philippe , Leaune Edouard
Published: December 2023

Background

The exposure to patient suicide (PS) has been identified as one of the most frequent and troubling professional experience for psychiatric trainees. Further studies are needed to better understand how residents cope with these experiences and the association between perceived support and the impacts of PS.

Method

In this qualitative study, we aimed to assess the impact of exposure to PS during psychiatric residency on trainees’ professional career and practical experience. A total of 19 French psychiatric residents participated in 4 focus-groups performed between November 2017 and May 2019.

Results

A total of 4 thematic clusters were identified through a five-step content analysis, namely: (a) reactions to the exposure; (b) coping strategies; (c) professional impact; and (d) prevention and postvention proposals. All participants described the critical impact of the support provided after PS, especially by their senior staff. Those who felt supported by their superior reported less negative impact, both in emotional and professional dimensions. Participants also shared proposals to improve the prevention and postvention issues related to the exposure to PS.

Conclusion

We performed the first qualitative study based on focus groups on the impact of PS on psychiatric residents, which allowed for an in-depth understanding of the participants’ lived experiences of the exposure to PS. The narratives inform the need and means to implement prevention and postvention strategies designed to buffer the negative impact of the exposure to PS in psychiatric trainees.