bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of Ki-67 Change Pattern After Core Needle Biopsy and Prognosis in HR+/HER2− Early Breast Cancer Patients
Authors: Li Shuai , Chen Xiaosong , Shen Kunwei
Published: June 2022

Background

To investigate the association of Ki-67 change pattern after core needle biopsy (CNB) and prognosis in HR+/HER2− early breast cancer patients.

Method

Eligible patients were categorized into three groups: Low group, Elevation group, and High group. Chi-square test and logistic regression analysis were used to compare the clinic-pathological characteristics. Kaplan–Meier method was used to estimate the rates of recurrence-free interval (RFI) and breast cancer-specific survival (BCSS), which were compared via the Log-rank test. Cox proportional hazard analysis was performed to investigate independent prognostic factors.

Results

A total of 2,858 patients were included: 1,179 (41.3%), 482 (16.9%), and 1,197 (41.8%) patients were classified into the low, elevation, and high groups, respectively. Age, tumor size, histological grade, lymph-vascular invasion (LVI), and ER level status were associated with Ki-67 change pattern after CNB. With a median follow-up of 53.6 months, the estimated 5-year RFI rates for the low group, elevation, and high groups were 96.4%, 95.3% and 90.9%, respectively (P < 0.001). And 5-year BCSS rates were 99.3%, 98.3% and 96.8%, respectively (P = 0.001). Compared with patients in the low group, patients in the high group had significantly worse RFI (hazard ratio [HR] 1.71, 95% confidence interval [CI] 1.16–2.54) in multivariate analysis.

Conclusions

Ki-67 change after CNB was associated with prognosis in HR+/HER2− early breast cancer. Patients with Ki-67 high or elevation after CNB had an inferior disease outcome, indicating the necessity of re-evaluating Ki-67 on surgical specimens after CNB.
Women in Neurosurgery: Historical Path to Self-Segregation and Proposal for an Integrated Future
Authors: Garozzo D. , Rispoli R. , Graziano F. , Gerardi R. M. , Grotenhuis A. , Jenkins A. , Sammons V. , Visocchi M. , Pinazzo S. , Lima R. , Martinez F. , Emamhadi M. , Pedro M. T. , Shirwari H. S. , Guedes F. , Bhagavatula I. D. , Shukla D. P. , Bhat I. D. , Ojo O. A. , Tirsit A. , Gonzales-Gonzales M. E. , Luna F. , Kretschmer T. , Benzel E. , Cappelletto B.
Published: June 2022

Despite the rising percentage of women accessing the medical profession over the last few decades, surgical specialties are still largely male-dominated; in particular, a remarkable gender disparity is evident in neurosurgery, where only 19% of practitioners are females. Although women may be reluctant to choose a challenging specialty like neurosurgery due to concerns around how to balance family and career, it must be admitted that prejudices against female neurosurgeons have been deeply rooted for long, prompting many to give up and switch track to less demanding subspecialties. Among those who have persisted, many, if not most, have experienced difficulties in career progression and received unequal treatment in comparison with their male counterparts. In 1989, a group of 8 female neurosurgeons founded Women in Neurosurgery (WINS), an organization that aimed to guarantee inclusivity in neurosurgery, encouraging a better and more egalitarian working environment. Thereafter, WINS sessions were regularly promoted at international conferences, offering female neurosurgeons a platform to report issues related to gender discrimination. Over recent years, the mission of WINS sessions in national and international conferences has taken an unexpected deviation; they have progressively become supplementary scientific sessions with only women neurosurgeons as speakers, thus paving the road to a form of self-segregation. This tendency has also resulted in the establishment of sections of only female neurosurgeons within some national societies. Although there remains a faction that fiercely supports the WINS mindset of reserved spaces for women, such segregation is an upsetting prospect for those who believe that science and professionalism have no gender; a growing part of the global neurosurgical community believes that the conception of a “female neurosurgery” and a “male neurosurgery” is misguided and counterproductive and consider the existence of the WINS as anachronistic and no longer necessary.
Efficacy and Safety of Camrelizumab in Combination with Docetaxel + S-1 Sequenced by Camrelizumab + S-1 for Stage III (PD-1+/MSI-H/EBV+/dMMR) Gastric Cancer: Study Protocol for a Single-Center, Prospective, Open-Label, Single-Arm Trial
Authors: Dong Zhongyi , Ni Bo , Yang Linxi , Guan Yujing , Zhu Chunchao , Zhao Enhao , Zhao Gang , Xia Xiang , Zhang Zizhen
Published: June 2022

Background

Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. A higher incidence of gastric cancer had been found in East Asia compared to the other regions. Gastrectomy with radical lymph node dissection is the cornerstone of curative treatment for Stage III gastric cancer, and postoperative systemic chemotherapy with docetaxel, S-1 improved patients’ disease-free survival rates. However, advances in immunotherapy bring innovations in the management of patients with gastric cancer. The objective of this study was to explore the efficacy and safety of camrelizumab in combination with docetaxel + S-1, sequenced by camrelizumab + S-1 in stage III gastric cancer patients who are EBV positive, with defective mismatch repair and CPS ≥5.

Methods and analysis

This prospective, open-label, single-arm trial was performed at Renji Hospital. In this study, a total of 70 adult patients aged 18–80 years with Stage III (PD-1+/MSI-H/EBV+/dMMR) gastric cancer confirmed by post-operative pathology will be enrolled after screening. Participants will receive the specific chemotherapy regimen until 1 year after the operation or until tumor recurrence or metastasis. The primary outcome is the 3-year disease-free survival rate measured by the Clopper-Pearson method and 95% confidence intervals. The secondary outcomes include overall survival, incidence and severity of adverse effects, and laboratory abnormalities. The data will be analyzed by the Kaplan-Meier method and log-rank test. The patients will be followed up every 3 months with imaging investigation until clinical remission.

Ethics and dissemination

All participants will provide informed consent. The protocol has been approved by the Shanghai Jiaotong University School of Medicine, Renji Hospital Ethics Committee (KY2019-191). The results will be disseminated through peer-reviewed manuscripts, reports and presentations.

Clinical Trial Registration

ClinicalTrials.gov, identifier: ChiCTR1900027123. Registration date November 2019; first enrolment December 2019; expected end date December 2021; trial status: Ongoing.

Brief Abstract

A clinical trial for Stage III (PD-1+/MSI-H/EBV+/dMMR) gastric cancer patients who accepted anti-PD-1 therapy combined with docetaxel + S-1 as the first-line treatment and explored improvements in three-year disease-free survival rate.
Osteoid Osteoma of the Proximal Femur: Pitfalls in Diagnosis and Performance of Open Surgical Resection
Authors: Zeng Hao , He Hongbo , Tong Xiaopeng , Wang Zhiwei , Luo Rongsheng , Liu Qing
Published: June 2022

Aims

Proximal femoral osteoid osteoma (OO) is extremely easy to be misdiagnosed or missed. The purpose of this study was to retrospectively analyze the clinical data of patients with proximal femoral OO in order to determine the clinical manifestation and imaging characteristics of the disease, so as to provide help for the preoperative diagnosis and clinical treatment of proximal femoral OO.

Methods

This was a retrospective study involving 35 patients with proximal femoral OO admitted into our hospital from January 2015 to January 2021. The baseline characteristics of the participants included; 24 males and 11 females, aged between 13 and 25 (mean 16.2) years old, and the course of the disease was 1 to 14 (mean 6.3) months. We used previous medical experience records of the patients to analyze for the causes of misdiagnosis. Moreover, we compared the difference between preoperative and postoperative treatment practices in alleviating pain in OO patients and restoring hip function. Follow-ups were carried out regularly, and patients advised to avoid strenuous exercises for 3 months.

Results

We followed up 35 patients (25 intercortical, 4 sub-periosteal, and 6 medullary) for an average of 41.4 months. We found that 15 patients (42.9%) had been misdiagnosed of synovitis, perthes disease, osteomyelitis, intra-articular infection, joint tuberculosis and hip impingement syndrome, whose average time from symptoms to diagnosis were 6.3 months. Postoperative pain score and joint function score improved significantly compared with preoperative, and complications were rare.

Conclusion

Open surgical resection constitutes an effective treatment for proximal femoral OO by accurately and completely removing the nidus. Wrong choice of examination, and the complexity and diversity of clinical manifestations constitutes the main reasons for the misdiagnosis of proximal femoral OO.
Study on the Correlation Between CT Features and Vascular Tumor Thrombus Together With Nerve Invasion in Surgically Resected Lung Adenocarcinoma
Authors: Song Yu , Chen Daiwen , Lian Duohuang , Xu Shangwen , Xiao Hui
Published: June 2022

Background

We aimed to analyze the relationship between pulmonary adenocarcinoma patients with vascular tumor thrombus and nerve invasion and different CT features.

Methods

The preoperative CT scanning data of 86 patients with lung adenocarcinoma who underwent surgical resection in our hospital from January 2020 to January 2022 were analyzed in the form of retrospective analysis. The CT images of all patients were observed, and the relationship between them and vascular tumor thrombus and nerve invasion of lung adenocarcinoma was analyzed. At the same time, the sensitivity, specificity, and accuracy of enhanced CT and plain CT were compared to evaluate the diagnostic efficacy of both.

Results

The results showed that the vascular tumor thrombus of lung adenocarcinoma was mainly related to the solid components and lobulated and calcified tumors in CT images, and the nerve invasion of lung adenocarcinoma was mainly related to the tumors with bronchial inflation sign in CT images (P < 0.05). The sensitivity, specificity, and accuracy of enhanced CT in the diagnosis of vascular tumor thrombus were 78.26%, 96.83%, and 91.86%, respectively, and the sensitivity, specificity, and accuracy in the diagnosis of nerve invasion were 75.00%, 98.72%, and 96.51%, respectively. The sensitivity, specificity, and accuracy of plain CT in the diagnosis of vascular tumor thrombus were 43.48%, 92.06%, and 79.07%, respectively, and the sensitivity, specificity, and accuracy in the diagnosis of nerve invasion were 25.00%, 94.87%, and 88.37%, respectively. The contrast showed that the sensitivity and accuracy of enhanced CT were higher than those of plain CT (P < 0.05), but the difference of specificity was not obvious (P > 0.05).

Conclusions

Solid components and lobulated and calcified tumors in CT signs are closely related to vascular tumor thrombus of lung adenocarcinoma, while patients with bronchial inflation sign are related to nerve invasion.
Genome-Wide Identification of RNA Editing Sites Affecting Muscle Development in Yak
Authors: Wu Xiaoyun , Chu Min , Ma Xiaoming , Pei Jie , Xiong Lin , Guo Xian , Liang Chunnian , Yan Ping
Published: June 2022

Skeletal muscle growth and development is a complicated process that is regulated at multiple steps and by numerous myogenesis genes. RNA editing represents one of the events at the post-transcriptional level, which contributes to the diversity of transcriptome and proteome by altering the nucleotides of RNAs. However, RNA editing events in the skeletal muscle of yaks are still not well defined. This study conducted whole-genome RNA-editing identification in skeletal muscle of yaks at embryonic stage (ES) and adult stage (AS). We found a total of 11,168 unique RNA editing sites, most of which were detected in the intergenic region. After annotation, we totally identified 2,718 editing sites within coding regions, among which 858 were missense changes. Moreover, totally 322 editing sites in the 3′ untranslated regions (UTR) were also predicted to alter the set of miRNA target sites, indicating that RNA editing may be involved in translational repression or mRNA degradation. We found 838 RNA editing sites (involving 244 common genes) that are edited differentially in ES as compared to AS. According to the KEGG enrichment analysis, these differentially edited genes were mainly involved in pathways highly related to skeletal muscle development and myogenesis, including MAPK, AMPK, Wnt, and PI3K-Akt signaling pathways. Altogether, our work presents the first characterization of RNA editing sites within yak skeletal muscles on a genome-wide scale and enhances our understanding of the mechanism of skeletal muscle development and myogenesis.
Pathophysiological and Pharmaceutical Considerations for Enhancing the Control of Sarcoptes scabiei in Wombats Through Improved Transdermal Drug Delivery
Authors: Bains Jaskaran , Carver Scott , Hua Susan
Published: June 2022

Sarcoptic scabiei is an invasive parasitic mite that negatively impacts wombats, causing sarcoptic mange disease, characterized by alopecia, intense pruritus, hyperkeratosis, and eventual mortality. Evidence suggests that wombats may be unable to recovery from infection without the assistance of treatments. Transdermal drug delivery is considered the most ideal route of administration for in situ treatment in free-ranging wombats, as it is non-invasive and avoids the need to capture affected individuals. Although there are effective antiparasitic drugs available, an essential challenge is adequate administration of drugs and sufficient drug retention and absorption when delivered. This review will describe the implications of sarcoptic mange on the physiology of wombats as well as discuss the most widely used antiparasitic drugs to treat S. scabiei (ivermectin, moxidectin, and fluralaner). The prospects for improved absorption of these drugs will be addressed in the context of pathophysiological and pharmaceutical considerations influencing transdermal drug delivery in wombats with sarcoptic mange.
Incremental Construction of Motorcycle Graphs
Authors: Aurenhammer Franz , Ladurner Christoph , Steinkogler Michael
Published: June 2022

We show that the so-called motorcycle graph of a planar polygon can be constructed by a randomized incremental algorithm that is simple and experimentally fast. Various test data are given, and a clustering method for speeding up the construction is proposed.
Effects of Vertical Smashing Rotary Tillage on Root Growth Characteristics and Yield of Broccoli
Authors: Shan Fake , Li Dongfang , Zhu Jianxi , Kang Shuo , Wang Jun
Published: June 2022

Most of the soils of the cultivated land in southern China are Ferralsols, which are easily deposited and hardened. To date, rotary tillage (RT) has been the major tillage system used in China. This tillage system results in a shallow soil pan, which reduces broccoli growth and yield. A two-year field experiment was conducted in the Central Zhejiang Basin, China, to compare the effects of vertical smashing rotary tillage (VSRT), RT, and vertical rotary tillage (VRT) on the soil properties, growth characteristics, and yield of broccoli. VSRT reduced the bulk density and penetration resistance of the 0–40 cm soil layer, and increased the soil water content of the 10–40 cm layer. Compared with RT and VRT, VSRT significantly promoted broccoli root length and increased broccoli root dry matter accumulation (DMA). VSRT significantly increased the DMA rate during the growth period, and the size of the broccoli florets was more uniform. In 2020, compared with RT and VRT, VSRT increased yields by 7.8% and 19.5%, respectively; while in 2021, the corresponding increases in yield due to VSRT were 24.8% and 40.5%. Therefore, VSRT, as a deep tillage method, can improve soil characteristics before planting broccoli and ultimately increase broccoli yield.
Plant Disease Detection and Classification Method Based on the Optimized Lightweight YOLOv5 Model
Authors: Wang Haiqing , Shang Shuqi , Wang Dongwei , He Xiaoning , Feng Kai , Zhu Hao
Published: June 2022

Traditional plant disease diagnosis methods are mostly based on expert diagnosis, which easily leads to the backwardness of crop disease control and field management. In this paper, to improve the speed and accuracy of disease classification, a plant disease detection and classification method based on the optimized lightweight YOLOv5 model is proposed. We propose an IASM mechanism to improve the accuracy and efficiency of the model, to achieve model weight reduction through Ghostnet and WBF structure, and to combine BiFPN and fast normalization fusion for weighted feature fusion to speed up the learning efficiency of each feature layer. To verify the effect of the optimized model, we conducted a performance comparison test and ablation test between the optimized model and other mainstream models. The results show that the operation time and accuracy of the optimized model are 11.8% and 3.98% higher than the original model, respectively, while F1 score reaches 92.65%, which highlight statistical metrics better than the current mainstream models. Moreover, the classification accuracy rate on the self-made dataset reaches 92.57%, indicating the effectiveness of the plant disease classification model proposed in this paper, and the transfer learning ability of the model can be used to expand the application scope in the future.
Control System of a Motor-Driven Precision No-Tillage Maize Planter Based on the CANopen Protocol
Authors: Chen Jincheng , Zhang Hui , Pan Feng , Du Mujun , Ji Chao
Published: June 2022

To reduce the cost of machinery and manual operation, greatly improve the efficiency of maize sowing, and solve the problems of slow sowing speed, unstable operation quality, and the difficult monitoring of the sowing process of traditional seeders, a control system for an electrically driven precision maize seeder based on the CANopen protocol was designed. In this system, an STM32 is used as the main controller, and the vehicle terminal is used to set the operating parameters, such as the spacing of sowing plants and the number of holes in the metering plate. The GPS receiver is used to collect the forward speed of the tractor. An infrared photoelectric sensor is used to monitor the working state of the seeder. In this study, tests were conducted on different evaluation indices. The results showed that the detection accuracy of the photoelectric sensor reached 99.8% and the fault alarm rate reached 100%. The qualified rate of sowing was more than 91.0%. Based on indoor test results, the qualified rate was higher when the grain spacing was larger. The field test showed, in terms of the seeding performance, that the control system had good stability. When the grain spacing was set to 20 cm and the operating speed was 6~12 km/h, the qualified index was more than 89% and the reseeding index was less than 1.93%. The variation in sowing performance between different monomers was small, and the seeding performance was good. The control system helps to improve the performance of the seeder.
Allelopathic Activity of a Novel Compound, 5,6-Dihydrogen-11α-O-acetyl-12β-O-tigloyl-17β-marsdenin, and a Known Steroidal Glycoside from the Leaves of Marsdenia tenacissima (Roxb.) Moon
Authors: Moh Seinn Moh , Iwasaki Arihiro , Suenaga Kiyotake , Kato-Noguchi Hisashi
Published: June 2022

Medicinal plants are rich sources of bioactive substances that can be used to develop environmentally friendly weed control alternatives. Marsdenia tenacissima (Roxb.) Moon is a traditional medicinal plant well known for its pharmacological activities and several bioactive compounds. However, its allelopathy and related substances have not been reported. Hence, the present study was conducted to explore the allelopathic potential and substances from M. tenacissima leaves. Aqueous methanol extracts of M. tenacissima showed significant inhibitory activities against the growth of cress (Lepidium sativum L.) and Italian ryegrass (Lolium multiflorum Lam.). The extracts were purified through various chromatography steps, and two allelopathic substances were isolated and determined by spectral data to be steroidal glycoside 1 (5,6-dihydrogen-11α-O-acetyl-12β-O-tigloyl-17β-marsdenin), a novel compound, and steroidal glycoside 2 (5,6-dihydrogen-11α,12β-di-O-tigloyl-17β-marsdenin). Both compounds significantly inhibited the growth of cress seedlings. Steroidal glycoside 1 showed 1.6- and 4-times greater growth inhibitory potential against the cress shoots and roots than steroidal glycoside 2. The concentrations needed for 50% growth inhibition of the cress seedling shoots and roots were 0.46 and 0.03 mM for steroidal glycoside 1, respectively, and 0.74 and 0.12 mM for steroidal glycoside 2, respectively. Therefore, these results suggest that steroidal glycosides 1 and 2 may be responsible for the allelopathy of the M. tenacissima leaves.
Investigating Contribution Factors of Grain Input to Output Transformation for the Inner Mongolia Autonomous Region in China
Authors: Zhu Yongchang , Liu Buchun , Liu Yuan , Shirazi Sana Zeeshan , Cui Cheng , He Jinna , Liu Shanshan , Yang Fan , Zhang Xiaonan
Published: June 2022

Inner Mongolia Autonomous Region (IMAR) has become the sixth-largest grain-producing province of China, contributing 5.6% of the total national grain output in 2021. Grain production increased by around 821% from 1980 to 2019. The contributions of structure adjustment, sown area, and yield to the increase in grain production was analyzed for IMAR. For yield, the Cobb–Douglas production function was estimated, to analyze the contribution of agricultural inputs (fertilizer, irrigation, seed, pesticide, agriculture film, and machinery operation) and the impact of natural hazards on yield. Results from our study showed that the grain production and yield in IMAR increased by 81.40 × 104 t and 0.11 t·ha−1 annually from 1980 to 2019. The grain-crop sown area increased by 73.65 × 103 ha annually, and the sown area of maize constitutes 16.82% to 55.30% of the total sown area of the IMAR in 1980–2019. In IMAR, sown area, yield, and structure adjustment can explain 54.06%, 33.31%, and 12.63% of the increase in the total grain production, respectively, from 1980 to 2019. The input per unit area of fertilizer, irrigation, and other inputs (seed, pesticide, agricultural film, and mechanical operation inputs) increased by 362.16%, 259.66%, and 405.55%, respectively, from 1981 to 2019, which contributed 160.44%, 15.06%, and 53.13% for the main cereal grain (maize and wheat) yield, respectively, while the decrease in the comprehensive loss rate from agrometeorological hazards contributed 7.76% to the main cereal grain yield in IMAR. Technologies such as water-saving irrigation, high-efficiency fertilizer application, and agrometeorological-hazards mitigation measures should be adopted in the future to increase production, considering the stable sown area and environmental and resource constraints.
Wheat Varietal Response to Relative SPAD Index (RSI) and Relative Normalized Difference Vegetation Index (RNDVI) under Variable Nitrogen Application and Terminal Heat Stress along with Yield Repercussion
Authors: Andleeb Tayyaba , Ali Zeshan , Mahmood Zahid , Latif Sadia , Quraishi Umar Masood
Published: June 2022

Nitrogen (N) deficiency and heat stress (HS) are major abiotic stresses that affect the quantity and quality of wheat grains. This study was conducted to examine wheat varietal response to RSI and RNDVI at the anthesis stage and their relationship to yield and yield-related traits under variable N supply and terminal heat stress. Twelve wheat varieties were evaluated in 2016–2017 and 2017–2018 at the National Agricultural Research Centre (NARC), Islamabad, Pakistan. The experiment was divided into three sets, i.e., N120 (120 kg N/ha), N60 (60 kg N/ha) and N0 (0 kg N/ha), based on the nitrogen fertilizer application. The physiological and yield-related parameters were recorded. Mean grain yield for all twelve varieties, averaged from two years of data, ranged between 1655.0 and 3890.1 kg/ha. Maximum RSI (0.99), RNDVI (1.03) and GY (3890.9 kg/ha) were recorded for FSD-08, while AARI-11 showed minimum RSI (0.50), RNDVI (0.56) and GY (1396.40 kg/ha). In the present study, mean CTD was lower, at N0 (3.57 °C), followed by N60 (5.07 °C) and N120 (5.47 °C) on average for the two years of data. The strong positive correlation of RSI and RNDVI with grain yield at R2 = 0.73 and R2 = 0.49 suggest that these parameters can be used as efficient and precise selection criteria for identifying nitrogen-use-efficient wheat varieties under terminal heat-stress conditions. This work will help the researchers to identify and develop nitrogen-use-efficient and thermos-tolerant wheat cultivars by minimizing the negative impacts of heat stress at the anthesis stage.
A Balanced Sowing Density Improves Quality of Rapeseed Blanket Seedling
Authors: Zuo Qingsong , You Jingjing , Wang Long , Zheng Jingdong , Li Jing , Qian Chen , Lin Guobing , Yang Guang , Leng Suohu
Published: June 2022

Mechanized transplanting of rapeseed (Brassica napus L.) blanket seedling is an effective strategy to cope with the seasonal conflict and large labor cost in rapeseed production. The sowing density is a key factor to cultivate high-quality seedlings suitable for mechanized transplanting. An experiment was conducted to investigate the effects of different sowing density levels of 638, 696, 754, 812, 870 and 928 seeds per tray (referred as D1, D2, D3, D4, D5 and D6, respectively) on agronomic traits and survival rate after mechanized transplanting of two rapeseed cultivars (Zheyouza108 and Heza17) in 2020 and 2021. The results showed that high sowing density increased plant height but decreased leaf area, collar diameter, biomass accumulation, the ratio of root to shoot and seedling fullness. These negative effects jointly decreased the seedling rate and survival rate after mechanized transplanting. However, the seedlings under D1 and D2 posed a great plant survival rate of more than 95% after mechanized transplanting, suggesting that the seedlings under the two densities were perfect for mechanized transplanting. In addition, hierarchical analysis grouped D1 and D2 into the same class, indicating that their seedling qualities were not significantly different, though the blanket seedlings under D1 outperformed those under D2 in some traits. A sowing density of 696 seeds per tray (D2) is then recommended in this study, altogether considering its high-quality seedlings suitable for mechanized transplanting, and economically, fewer seedling trays required.
Use of Sentinel-2 Derived Vegetation Indices for Estimating fPAR in Olive Groves
Authors: Leolini Luisa , Moriondo Marco , Rossi Riccardo , Bellini Edoardo , Brilli Lorenzo , López-Bernal Álvaro , Santos Joao A. , Fraga Helder , Bindi Marco , Dibari Camilla , Costafreda-Aumedes Sergi
Published: June 2022

Olive tree cultivation is currently a dominant agriculture activity in the Mediterranean basin, where the increasing impact of climate change coupled with the inefficient management of olive groves is negatively affecting olive oil production and quality in some marginal areas. In this context, satellite imagery may help to monitor crop growth under different environmental conditions, thus providing useful information for optimizing olive grove management and final production. However, the spatial resolution of freely-available satellite products is not yet adequate to estimate plant biophysical parameters in complex agroecosystems such as olive groves, where both olive trees and grass cover contribute to the vegetation indices (VIs) signal at pixel scale. The aim of this study is therefore to test a disentangling procedure to partition the VIs signal among the different components of the agroecosystem to use this information for the monitoring of olive growth processes during the season. Specifically, five VIs (GEMI, MCARI2, NDVI, OSAVI, MCARI2/OSAVI) as recorded by Sentinel-2 at a spatial resolution of 10 m over five olive groves in the Montalbano area (Tuscany, Central Italy), were tested as a proxy for olive tree intercepted radiation. The olive tree volume per pixel was initially used to linearly rescale the VIs signal into the relevant value for the grass cover and olive trees. The models, describing the relationship between rescaled VIs and observed fraction of Photosynthetically Active Radiation (fPAR), were fitted and then validated against independent datasets. While in the calibration phase, a greater robustness at predicting fPAR was obtained using NDVI (r = 0.96 and RRMSE = 9.86), the validation results demonstrating that GEMI and MCARI2/OSAVI provided the highest performances (GEMI: r = 0.89 and RRMSE = 21.71; MCARI2/OSAVI: r = 0.87 and RRMSE = 25.50), in contrast to MCARI2 that provided the lowest (r = 0.67 and RRMSE = 36.78). These results may be related to the VIs’ intrinsic features (e.g., lower sensitivity to atmosphere and background effects), which make some of these indices, compared to others, less sensitive to saturation effects by improving fPAR estimation (e.g., GEMI vs. NDVI). On this basis, this study evidenced the need to improve the current methodologies to reduce inter-row effects and select appropriate VIs for fPAR estimation, especially in complex agroecosystems where inter-row grass growth may affect remote sensed-derived VIs signal at an inadequate pixel resolution.
Fatty Acid Content of Four Salmonid Fish Consumed by Indigenous Peoples from the Yamal-Nenets Autonomous Okrug (Northwestern Siberia, Russia)
Authors: Dvoretsky Alexander G. , Bichkaeva Fatima A. , Vlasova Olga S. , Andronov Sergei V. , Dvoretsky Vladimir G.
Published: June 2022

We assayed fatty acids in the flesh of Arctic cisco Coregonus autumnalis (adult and juvenile), least cisco Coregonus sardinella, muksun Coregonus muksun, and Arctic charr Salvelinus alpinus inhabiting water bodies of the Gydan Peninsula, Siberia, Russia. The highest concentrations of total and polyunsaturated fatty acids (PUFAs) were found in Arctic charr (27.8 and 9.5 mg g−1) and adult Arctic cisco (20.2 and 7.6 mg g−1), while the lowest concentrations occurred in juvenile Arctic cisco (7.5 and 3.6 mg g−1). Multivariate analyses divided all studied fish into five distinct groups with the highest similarity between least cisco and muksun and the highest dissimilarity between juvenile Arctic cisco and Arctic charr. Coregonid fish from the study area had a higher content of docosahexaenoic and eicosapentaenoic acids than their conspecifics from subarctic and temperate habitats. The flesh of the studied fish is a source of a healthy diet for humans. Taking into account that all the studied fish are components of the traditional diet of indigenous peoples in northwestern Siberia, our data may be useful not only for local consumers and anglers but also for stakeholders focused on food policy and food security in the area.
Retrospective Evaluation of Method of Treatment, Laboratory Findings, and Concurrent Diseases in Dairy Cattle Diagnosed with Left Displacement of the Abomasum during Time of Hospitalization
Authors: Tschoner Theresa , Zablotski Yury , Feist Melanie
Published: June 2022

Left displacement of the abomasum (LDA) is a disease often diagnosed in high-producing dairy cattle, resulting in direct and in indirect costs for the farmer, and discomfort and death for the cows. For the present retrospective study, the aims were to assess the effect of treatment on recovery during the time of hospitalization of the cows, to investigate the influence of concurrent diseases on the recovery, and to identify prognostic indicators in laboratory findings. Metritis/endometritis (38.4% of cows) was the concurrent disease diagnosed most often. Conservative treatment (abomasal rolling) was performed successfully in 92.8% of cows; the recurrence rate was 56.7%. Neither treatment with an oral drench nor treatment with analgesics had any influence on the recurrence of LDA following abomasal rolling during hospitalization. Endoscopic abomasopexy as described by Janowitz was performed more often than right flank laparotomy (40.8% and 40.2%, respectively). A significantly (p < 0.01) higher number of cows had the outcome “recovery” compared with “death”. The results of this study show that the outcome after surgery for LDA under clinical conditions does not depend on the method of surgery. Moreover, cows with LDA often suffer from concurrent diseases. If conservative treatment is decided on, farmers should be informed that there is a high recurrence rate, and other treatment options should be discussed.
Feeding Fiber-Bound Polyphenol Ingredients at Different Levels Modulates Colonic Postbiotics to Improve Gut Health in Cats
Authors: Jewell Dennis E. , Jackson Matthew I. , Cochrane Chun-Yen , Badri Dayakar V.
Published: June 2022

Consumption of fiber in its different forms can result in positive health effects. Prior studies in dogs found that addition of a fiber bundle (composed of pecan shells, flax seed, and powders of cranberry, citrus, and beet) to food resulted in a shift in fecal bacterial metabolism from proteolysis to saccharolysis. The present study evaluated the changes in fecal metabolites and microbiota in healthy cats following the consumption of this fiber bundle. Following a 28-day pre-feed period, 56 healthy adult cats received food with none or one of three concentrations (0%, 1%, 2%, and 4%) of the fiber bundle for a 31-day period. In cats that consumed the 4% fiber bundle, levels of ammonium and fecal branched-chain fatty acids (BCFAs) decreased from baseline and compared with the other groups. Addition of any level of the fiber bundle resulted in increases in beneficial metabolites: polyphenols hesperidin, hesperetin, ponciretin, secoisolariciresinol diglucoside, secoisolariciresinol, and enterodiol. Little change in fecal microbiota was observed. Since higher levels of ammonia and BCFAs indicate putrefactive metabolism, the decreases in these with the 4% fiber bundle indicate a shift toward saccharolytic metabolism despite little change in the microbiota composition.
Optimization of Milk Substitutes for the Artificial Rearing of Chinese Tree Shrews (Tupaia belangeri chinensis)
Authors: Chen Jia-Qi , Zhang Qingyu , Yu Dandan , Bi Rui , Ma Yuhua , Li Yijiang , Lv Long-Bao , Yao Yong-Gang
Published: June 2022

The Chinese tree shrew (Tupaia belangeri chinensis) has the potential to replace the use of non-human primates in biomedical research. To increase the availability of this species, we have undertaken the ambitious task of establishing inbred lines of the Chinese tree shrew; however, we have been hindered by a low survival rate of inbred pups. Here, we report our artificial rearing (AR) of Chinese tree shrew pups using four different milk substitutes: the formula described by Tsang and Collins (milk TC) and three commercially available milk substitutes intended for possums (milk A and milk C) and for guinea pigs (milk B). We compared the effects of these milk substitutes and maternal milk on the daily milk consumption, growth performance, and survival of the pups. We also assessed the life span and reproductive performance of the F1 individuals given the best milk substitute as compared to the maternally reared (MR) pups. Milk B was found to be appropriate for AR. Pups fed with milk B had a high survival rate at the weaning age compared to those fed with the other milk substitutes. The AR pups fed with milk B had a life span similar to that of MR pups. AR females fed with milk B had an earlier age of the first reproduction, a larger number of litters, and a higher rate of survival of the offspring at the weaning age compared with the MR females. The successful optimization of a milk substitute for AR of Chinese tree shrew pups will undoubtedly facilitate the wide usage of this experimental animal.
Deciphering the Contribution of BP230 Autoantibodies in Bullous Pemphigoid
Authors: Cole Connor , Borradori Luca , Amber Kyle T.
Published: June 2022

Bullous pemphigoid (BP) is a subepidermal autoimmune blistering disease predominantly affecting elderly patients and carries significant morbidity and mortality. Patients typically suffer from severe itch with eczematous lesions, urticarial plaques, and/or tense blisters. BP is characterized by the presence of circulating autoantibodies against two components of the hemidesmosome, BP180 and BP230. The transmembrane BP180, also known as type XVII collagen or BPAG2, represents the primary pathogenic autoantigen in BP, whereas the intracellular BP230 autoantigen is thought to play a minor role in disease pathogenesis. Although experimental data exist suggesting that anti-BP230 antibodies are secondarily formed following initial tissue damage mediated by antibodies targeting extracellular antigenic regions of BP180, there is emerging evidence that anti-BP230 IgG autoantibodies alone directly contribute to tissue damage. It has been further claimed that a subset of patients has a milder variant of BP driven solely by anti-BP230 autoantibodies. Furthermore, the presence of anti-BP230 autoantibodies might correlate with distinct clinical features. This review summarizes the current understanding of the role of BP230 and anti-BP230 antibodies in BP pathogenesis.
Comparative Evaluation of Vitek 2 and Etest versus Broth Microdilution for Ceftazidime/Avibactam and Ceftolozane/Tazobactam Susceptibility Testing of Enterobacterales and Pseudomonas aeruginosa
Authors: Papadomanolaki Arhodoula , Siopi Maria , Karakosta Polyxeni , Vourli Sophia , Pournaras Spyros
Published: June 2022

Ceftazidime/avibactam (CZA) and ceftolozane/tazobactam (C/T) are novel antibiotics with activity against multidrug-resistant Gram-negative pathogens. Nevertheless, resistance to both agents has been reported emphasizing the need for accurate and widely accessible susceptibility testing. In the present study, Vitek 2 and Etest CAZ and C/T MIC results for 100 non-repetitive clinical isolates (83 Enterobacterales and 17 P. aeruginosa, whereof 69 challenge isolates) were compared to the standard broth microdilution (BMD) method. EUCAST breakpoints were used for assessing the categorical (CA) and essential (EA) agreement between the methods along with the corresponding error rates. The Vitek 2 performance was comparable to that of BMD for testing both antimicrobial agents exceeding the ISO requirements (CA 98–99%, EA 96–100%, major errors (MEs) 0–1%, very major error (VMEs) 1%). Likewise, the Etest provided accurate results for CZA and C/T testing against Enterobacterales and P. aeruginosa, respectively (CA 100%, EA 97–100%, MEs 0%, VMEs 0%). On the contrary, EA of 85% and 6% VME rate were found for CZA Etest and P. aeruginosa. Overall, Vitek 2 measurements of CZA and C/T susceptibility correlated closely with the reference BMD, indicating that it can represent a suitable alternative to BMD for susceptibility testing of Enterobacterales and P. aeruginosa. The Etest did not fulfill the ISO performance criteria of EA and VME for CZA and P. aeruginosa. Further studies are needed to assess whether the Etest allows a reliable assessment of CZA and C/T EUCAST MICs.
Infection Sources and Klebsiella pneumoniae Antibiotic Susceptibilities in Endogenous Klebsiella Endophthalmitis
Authors: Chen Kuan-Jen , Chen Yen-Po , Chen Yi-Hsing , Liu Laura , Wang Nan-Kai , Chao An-Ning , Wu Wei-Chi , Hwang Yih-Shiou , Chou Hung-Da , Kang Eugene Yu-Chuan , Chen Yen-Ting , Sun Ming-Hui , Lai Chi-Chun
Published: June 2022

Endogenous endophthalmitis is an uncommon intraocular infection with potentially devastating consequences on vision. Klebsiella pneumoniae is highly prevalent in East Asian countries, with an increasing incidence recently worldwide. This retrospective study investigates infection sources and antibiotic susceptibilities of K. pneumoniae in patients with endogenous K. pneumoniae endophthalmitis (EKE) in Northern Taiwan. One hundred and fifty-seven patients with EKE were reviewed between January 1996 and April 2019. Pyogenic liver abscess (120/157, 76.4%) was the most common infection source, followed by pneumonia (13, 8.3%), urinary tract infection (7, 4.5%), and intravenous drug use (4, 2.5%). Bilateral involvement was identified in 12.1% (19/157) of patients, especially in patients with pyogenic liver abscess (16/120, 13.3%), pneumonia (2/13, 15.4%), and urinary tract infection (1/7, 14.3%). The antibiotic susceptibility rates were 98.1%, 92.5%, 97.5%, 96.8%, 100%, 99.3%, and 100% for amikacin, cefuroxime, ceftazidime, ceftriaxone, carbapenems, ciprofloxacin, and levofloxacin, respectively. Four extended-spectrum β-lactamase-producing multidrug-resistant (MDR) K. pneumoniae isolates were identified. In conclusion, pyogenic liver abscess was the major infection source in EKE. In addition, K. pneumoniae was still highly susceptible to ceftazidime and amikacin, and the MDR K. pneumoniae isolates were not common in EKE.
Comparative Physiological and Transcriptomic Analyses Reveal Mechanisms of Exogenous Spermidine-Induced Tolerance to Low-Iron Stress in Solanum lycopersicum L
Authors: Shi Yu , Zhao Yihong , Yao Qi , Liu Feng , Li Xiumin , Jin Xiu , Zhang Yi , Ahammed Golam Jalal
Published: June 2022

Iron (Fe) deficiency in plants is a major problem in agriculture. Therefore, we investigated both the physiological features and molecular mechanisms of plants’ response to low-Fe (LF) stress along with the mitigation of LF with exogenous spermidine (Spd) in tomato plants. The results showed that exogenous Spd foliar application relieved the suppressing effect of LF stress on tomato plants by regulating the photosynthetic efficiency, chlorophyll metabolism, antioxidant levels, organic acid secretion, polyamine metabolism and osmoregulatory systems. Analysis of transcriptomic sequencing results revealed that the differentially expressed genes of iron-deficiency stress were mainly enriched in the pathways of phytohormone signaling, starch and sucrose metabolism and phenyl propane biosynthesis in both leaves and roots. Moreover, Spd-induced promotion of growth under LF stress was associated with upregulation in the expression of some transcription factors that are related to growth hormone response in leaves (GH3, SAUR, ARF) and ethylene-related signaling factors in roots (ERF1, ERF2). We propose that traits associated with changes in low-iron-tolerance genes can potentially be used to improve tomato production. The study provides a theoretical basis for dealing with the iron deficiency issue to develop efficient nutrient management strategies in protected tomato cultivation.
Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells
Authors: Xia Huan , Wang Yongmei , Dai Jie , Zhang Xin , Zhou Jun , Zeng Zhu , Jia Yi
Published: June 2022

Selenoprotein K (SELENOK) is an endoplasmic reticulum stress (ERS)-regulated protein required for the calcium (Ca2+) flux-mediated migration of T cells and neutrophils, and the migration and phagocytosis of macrophages and microglia. However, the effect of SELENOK on the regulation of the immune function of dendritic cells (DCs), including immature DCs (imDCs) and mature DCs (mDCs), is still unclear. In this study, imDCs prepared from SELENOK knockout mice were used to evaluate the effect of SELENOK on the migration and phagocytosis of imDCs. The results showed that ERS-induced downregulation of imDCs phenotypic markers led to a reduction in Ras homolog gene family member A (RhoA)-dependent migration and enhanced Ca2+/CD205-mediated phagocytosis. SELENOK deficiency-induced upregulation of selenoprotein S (SELENOS) attenuated ERS levels in imDCs. An increase in Ca2+ levels resulted in increased migration and decreased phagocytosis with or without ERS conditions. The migration was RhoA-dependent, and Ca2+ or CD205 was associated with regulating phagocytosis in imDCs. Our study found that SELENOK is required for imDC migration and phagocytosis.
A Greater Improvement of Intrahepatic Fat Contents after 6 Months of Lifestyle Intervention Is Related to a Better Oxidative Stress and Inflammatory Status in Non-Alcoholic Fatty Liver Disease
Authors: Monserrat-Mesquida Margalida , Quetglas-Llabrés Magdalena , Bouzas Cristina , Montemayor Sofía , Mascaró Catalina M. , Casares Miguel , Llompart Isabel , Gámez José M. , Tejada Silvia , Martínez J. Alfredo , Tur Josep A. , Sureda Antoni
Published: June 2022

Non-alcoholic fatty liver disease (NAFLD) is a disorder characterized by the excessive accumulation of lipids in the liver parenchyma. To date, there is no effective pharmacological treatment against NAFLD. Objective: To assess the relationship between the improvement of the intrahepatic fat content (IFC) in patients with NAFLD and metabolic syndrome and biomarkers of oxidative stress and inflammation after 6 months of lifestyle intervention. Patients diagnosed with NAFLD (n = 60 adults; 40–60 years old) residing in the Balearic Islands, Spain, were distributed in tertiles attending the improvement of IFC calculated by magnetic resonance imaging (MRI). Anthropometrics, blood pressure, maximal oxygen uptake, and pro/antioxidant and inflammatory biomarkers were determined in plasma before and after the lifestyle intervention. The improvement in IFC levels was higher in tertile 3 with respect to tertiles 2 and 1. The greatest improvement in IFC is related to cardiorespiratory fitness and adherence to the Mediterranean diet (ADM). Higher reductions in weight, body mass index (BMI), and alanine aminotransferase (ALT) were observed in tertile 3 with respect to tertile 1 after 6 months of intervention. The improvement in catalase, irisin, and cytokeratin 18 plasma levels were higher in tertile 3, whereas no differences were observed in superoxide dismutase activity. Malondialdehyde and protein carbonyl levels, as biomarkers of oxidative damage, remained unchanged in all groups. The present data show that the reduction of IFC is associated with an improvement in pro/antioxidant and pro-inflammatory status and a better cardiorespiratory fitness in NAFLD patients.
Opioids and Vitamin C: Known Interactions and Potential for Redox-Signaling Crosstalk
Authors: Newman Mackenzie , Connery Heather , Boyd Jonathan
Published: June 2022

Opioids are among the most widely used classes of pharmacologically active compounds both clinically and recreationally. Beyond their analgesic efficacy via μ opioid receptor (MOR) agonism, a prominent side effect is central respiratory depression, leading to systemic hypoxia and free radical generation. Vitamin C (ascorbic acid; AA) is an essential antioxidant vitamin and is involved in the recycling of redox cofactors associated with inflammation. While AA has been shown to reduce some of the negative side effects of opioids, the underlying mechanisms have not been explored. The present review seeks to provide a signaling framework under which MOR activation and AA may interact. AA can directly quench reactive oxygen and nitrogen species induced by opioids, yet this activity alone does not sufficiently describe observations. Downstream of MOR activation, confounding effects from AA with STAT3, HIF1α, and NF-κB have the potential to block production of antioxidant proteins such as nitric oxide synthase and superoxide dismutase. Further mechanistic research is necessary to understand the underlying signaling crosstalk of MOR activation and AA in the amelioration of the negative, potentially fatal side effects of opioids.