bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
Authors: Horvatić Anita , Gelemanović Andrea , Pirkić Boris , Smolec Ozren , Beer Ljubić Blanka , Rubić Ivana , Eckersall Peter David , Mrljak Vladimir , McLaughlin Mark , Samardžija Marko , Lipar Marija
Published: October 2021

Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approaches. To identify molecular networks and pathways which may play important roles in intervertebral disc herniation, as well as to reveal the potential features which could be useful for monitoring disease progression and prognosis, multi-omics profiling, including high-resolution liquid chromatography-mass spectrometry (LC-MS)-based metabolomics and tandem mass tag (TMT)-based proteomics was performed. Cerebrospinal fluid of nine dogs with IVDH and six healthy controls were used for the analyses, and an additional five IVDH samples were used for proteomic data validation. Furthermore, multi-omics data were integrated to decipher a complex interaction between individual omics layers, leading to an improved prediction model. Together with metabolic pathways related to amino acids and lipid metabolism and coagulation cascades, our integromics prediction model identified the key features in IVDH, namely the proteins follistatin Like 1 (FSTL1), secretogranin V (SCG5), nucleobindin 1 (NUCB1), calcitonin re-ceptor-stimulating peptide 2 precursor (CRSP2) and the metabolites N-acetyl-D-glucosamine and adenine, involved in neuropathic pain, myelination, and neurotransmission and inflammatory response, respectively. Their clinical application is to be further investigated. The utilization of a novel integrative interdisciplinary approach may provide new opportunities to apply innovative diagnostic and monitoring methods as well as improve treatment strategies and personalized care for patients with degenerative spinal disorders.
Targeted Phytohormone Profiling Identifies Potential Regulators of Spikelet Sterility in Rice under Combined Drought and Heat Stress
Authors: Da Costa Maria Vera Jesus , Ramegowda Venkategowda , Sreeman Sheshshayee , Nataraja Karaba N.
Published: October 2021

Rice cultivated under rainfed or semi-irrigated ecosystems is frequently exposed to a combination of drought and heat stress. As a sensitive crop at the reproductive stage, exposure to combined drought and heat stress will have a deleterious effect on yield. In this study, two rice cultivars with contrasting spikelet sterility, AVT2-5315 (low sterility) and AC35027 (high sterility), under combined stress were selected for physiological characterization and phytohormonal profiling at anthesis. Under combined stress, both cultivars did not differ in the physiological parameters such as relative water content, photosynthetic rate, light-adapted chlorophyll fluorescence and biomass, suggesting a similar source activity under stress. However, AVT2-5315 showed better yield due to better pollen and spikelet fertility than AC35027, suggesting its intrinsic tolerance ability under combined stress. Targeted profiling of 15 phytohormones from drought, heat and combined stress-treated flag leaf and spikelet tissues using LC–MS/MS showed increased accumulation of auxins (indole 3-acetic acid and indole 3-butyric acid) in flag leaves and jasmonic acid in spikelets of AVT2-5315, while there was increased accumulation of ethylene in flag leaves and methyl-jasmonate in spikelets of AC35027. Increased accumulation of these hormones correlated with key biosynthetic pathway genes. In the flag leaves, increased accumulation of auxins was correlated with increased transcript levels of YUCCA-like gene 1 (OsYUCCA1) and fish bone (OsFIB), in AVT2-5315 under combined stress. In AC35027, increased ethylene content was correlated with expression of 1-aminocyclopropane-1-carboxylate synthase 1 (OsASC1) and aminocyclopropane-1-carboxylic acid oxidase 2 (OsACO2). Similarly, in spikelets, increased accumulation of jasmonic acid in AVT2-5315 was correlated with expression of allene oxide cyclase (OsAOC) and 12-oxophytodienoic acid reductase 1 (OsOPR1). The mechanism of regulating spikelet sterility by these hormones needs further investigation towards improving rice tolerance to combined stress.
Involvement of Ghrelin Dynamics in Stress-Induced Eating Disorder: Effects of Sex and Aging
Authors: Yamada Chihiro
Published: October 2021

Stress, a factor that affects appetite in our daily lives, enhances or suppresses appetite and changes palatability. However, so far, the mechanisms underlying the link between stress and eating have not been fully elucidated. Among the peripherally produced appetite-related peptides, ghrelin is the only orexigenic peptide, and abnormalities in the dynamics and reactivity of this peptide are involved in appetite abnormalities in various diseases and psychological states. This review presents an overview of the research results of studies evaluating the effects of various stresses on appetite. The first half of this review describes the relationship between appetite and stress, and the second half describes the relationship between the appetite-promoting peptide ghrelin and stress. The effects of sex differences and aging under stress on appetite are also described.
Pathogenesis of Endometriosis: New Insights into Prospective Therapies
Authors: Kapoor Radhika , Stratopoulou Christina Anna , Dolmans Marie-Madeleine
Published: October 2021

Endometriosis is a female reproductive disorder characterized by growth of uterine cells and tissue in distant sites. Around 2–10% of women experience this condition during reproductive age, 35–50% of whom encounter fertility issues or pain. To date, there are no established methods for its early diagnosis and treatment, other than surgical procedures and scans. It is difficult to identify the disease at its onset, unless symptoms such as infertility and/or pain are present. Determining the mechanisms involved in its pathogenesis is vital, not only to pave the way for early identification, but also for disease management and development of less invasive but successful treatment strategies. Endometriosis is characterized by cell proliferation, propagation, evasion of immunosurveillance, and invasive metastasis. This review reports the underlying mechanisms that are individually or collectively responsible for disease establishment and evolution. Treatment of endometriosis mainly involves hormone therapies, which may be undesirable or have their own repercussions. It is therefore important to devise alternative strategies that are both effective and cause fewer side effects. Use of phytochemicals may be one of them. This review focuses on pharmacological inhibitors that can be therapeutically investigated in terms of their effects on signaling pathways and/or mechanisms involved in the pathogenesis of endometriosis.
Mechanistic Insight from Preclinical Models of Parkinson’s Disease Could Help Redirect Clinical Trial Efforts in GDNF Therapy
Authors: Delgado-Minjares Karen M. , Martinez-Fong Daniel , Martínez-Dávila Irma A. , Bañuelos Cecilia , Gutierrez-Castillo M. E. , Blanco-Alvarez Víctor Manuel , Cardenas-Aguayo Maria-del-Carmen , Luna-Muñoz José , Pacheco-Herrero Mar , Soto-Rojas Luis O.
Published: October 2021

Parkinson’s disease (PD) is characterized by four pathognomonic hallmarks: (1) motor and non-motor deficits; (2) neuroinflammation and oxidative stress; (3) pathological aggregates of the α-synuclein (α-syn) protein; (4) neurodegeneration of the nigrostriatal system. Recent evidence sustains that the aggregation of pathological α-syn occurs in the early stages of the disease, becoming the first trigger of neuroinflammation and subsequent neurodegeneration. Thus, a therapeutic line aims at striking back α-synucleinopathy and neuroinflammation to impede neurodegeneration. Another therapeutic line is restoring the compromised dopaminergic system using neurotrophic factors, particularly the glial cell-derived neurotrophic factor (GDNF). Preclinical studies with GDNF have provided encouraging results but often lack evaluation of anti-α-syn and anti-inflammatory effects. In contrast, clinical trials have yielded imprecise results and have reported the emergence of severe side effects. Here, we analyze the discrepancy between preclinical and clinical outcomes, review the mechanisms of the aggregation of pathological α-syn, including neuroinflammation, and evaluate the neurorestorative properties of GDNF, emphasizing its anti-α-syn and anti-inflammatory effects in preclinical and clinical trials.
Trace Element Selenium Effectively Alleviates Intestinal Diseases
Authors: Ye Ruihua , Huang Jiaqiang , Wang Zixu , Chen Yaoxing , Dong Yulan
Published: October 2021

Selenium (Se) is an essential trace element in the body. It is mainly used in the body in the form of selenoproteins and has a variety of biological functions. Intestinal diseases caused by chronic inflammation are among the most important threats to human health, and there is no complete cure at present. Due to its excellent antioxidant function, Se has been proven to be effective in alleviating intestinal diseases such as inflammatory bowel diseases (IBDs). Therefore, this paper introduces the role of Se and selenoproteins in the intestinal tract and the mechanism of their involvement in the mediation of intestinal diseases. In addition, it introduces the advantages and disadvantages of nano-Se as a new Se preparation and traditional Se supplement in the prevention and treatment of intestinal diseases, so as to provide a reference for the further exploration of the interaction between selenium and intestinal health.
Epithelial-Cell-Derived Extracellular Vesicles in Pathophysiology of Epithelial Injury and Repair in Chronic Rhinosinusitis: Connecting Immunology in Research Lab to Biomarkers in Clinics
Authors: Takahashi Toru , Schleimer Robert P
Published: October 2021

Epithelial barrier disruption and failure of epithelial repair by aberrant epithelial-mesenchymal transition (EMT)-induced basal cells observed in nasal mucosa of chronic rhinosinusitis (CRS) are speculated to play important roles in disease pathophysiology. Microparticles (MPs) are a type of extracellular vesicle (EV) released by budding or shedding from the plasma membrane of activated or apoptotic cells. MPs are detected in nasal lavage fluids (NLFs) and are now receiving attention as potential biomarkers to evaluate the degree of activation of immune cells and injury of structural cells in nasal mucosa of subjects with sinus disease. There are three types of epithelial-cell-derived MPs, which are defined by the expression of different epithelial specific markers on their surface: EpCAM, E-cadherin, and integrin β6 (ITGB6). When these markers are on MPs that are also carrying canonical EMT/mesenchymal markers (Snail (SNAI1); Slug (SNAI2); alpha-smooth muscle actin (αSMA, ACTA2)) or pro- and anti-coagulant molecules (tissue factor (TF); tissue plasminogen activator (tPA); plasminogen activator inhibitor-1 (PAI-1)), they provide insight as to the roles of epithelial activation for EMT or regulation of coagulation in the underlying disease. In this review, we discuss the potential of epithelial MPs as research tools to evaluate status of nasal mucosae of CRS patients in the lab, as well as biomarkers for management and treatment of CRS in the clinic.
Decline in Constitutive Proliferative Activity in the Zebrafish Retina with Ageing
Authors: Hernández-Núñez Ismael , Quelle-Regaldie Ana , Sánchez Laura , Adrio Fátima , Candal Eva , Barreiro-Iglesias Antón
Published: October 2021

It is largely assumed that the teleost retina shows continuous and active proliferative and neurogenic activity throughout life. However, when delving into the teleost literature, one finds that assumptions about a highly active and continuous proliferation in the adult retina are based on studies in which proliferation was not quantified in a comparative way at the different life stages or was mainly studied in juveniles/young adults. Here, we performed a systematic and comparative study of the constitutive proliferative activity of the retina from early developing (2 days post-fertilisation) to aged (up to 3–4 years post-fertilisation) zebrafish. The mitotic activity and cell cycle progression were analysed by using immunofluorescence against pH3 and PCNA, respectively. We observed a decline in the cell proliferation in the retina with ageing despite the occurrence of a wave of secondary proliferation during sexual maturation. During this wave of secondary proliferation, the distribution of proliferating and mitotic cells changes from the inner to the outer nuclear layer in the central retina. Importantly, in aged zebrafish, there is a virtual disappearance of mitotic activity. Our results showing a decline in the proliferative activity of the zebrafish retina with ageing are of crucial importance since it is generally assumed that the fish retina has continuous proliferative activity throughout life.
Towards a Newborn Screening Common Data Model: The Utah Newborn Screening Data Model
Authors: Jones David , Shao Jianyin , Wallis Heidi , Johansen Cody , Hart Kim , Pasquali Marzia , Gouripeddi Ramkiran , Rohrwasser Andreas
Published: October 2021

As newborn screening programs transition from paper-based data exchange toward automated, electronic methods, significant data exchange challenges must be overcome. This article outlines a data model that maps newborn screening data elements associated with patient demographic information, birthing facilities, laboratories, result reporting, and follow-up care to the LOINC, SNOMED CT, ICD-10-CM, and HL7 healthcare standards. The described framework lays the foundation for the implementation of standardized electronic data exchange across newborn screening programs, leading to greater data interoperability. The use of this model can accelerate the implementation of electronic data exchange between healthcare providers and newborn screening programs, which would ultimately improve health outcomes for all newborns and standardize data exchange across programs.
COVID-19 Contact Tracing and Detection-Based on Blockchain Technology
Authors: Torky Mohamed , Goda Essam , Snasel Vaclav , Hassanien Aboul Ella
Published: October 2021

The fight against the COVID-19 pandemic still involves many struggles and challenges. The greatest challenge that most governments are currently facing is the lack of a precise, accurate, and automated mechanism for detecting and tracking new COVID-19 cases. In response to this challenge, this study proposes the first blockchain-based system, called the COVID-19 contact tracing system (CCTS), to verify, track, and detect new cases of COVID-19. The proposed system consists of four integrated components: an infection verifier subsystem, a mass surveillance subsystem, a P2P mobile application, and a blockchain platform for managing all transactions between the three subsystem models. To investigate the performance of the proposed system, CCTS has been simulated and tested against a created dataset consisting of 300 confirmed cases and 2539 contacts. Based on the metrics of the confusion matrix (i.e., recall, precision, accuracy, and F1 Score), the detection evaluation results proved that the proposed blockchain-based system achieved an average of accuracy of 75.79% and a false discovery rate (FDR) of 0.004 in recognizing persons in contact with COVID-19 patients within two different areas of infection covered by GPS. Moreover, the simulation results also demonstrated the success of the proposed system in performing self-estimation of infection probabilities and sending and receiving infection alerts in P2P communications in crowds of people by users. The infection probability results have been calculated using the binomial distribution function technique. This result can be considered unique compared with other similar systems in the literature. The new system could support governments, health authorities, and citizens in making critical decisions regarding infection detection, prediction, tracking, and avoiding the COVID-19 outbreak. Moreover, the functionality of the proposed CCTS can be adapted to work against any other similar pandemics in the future.
The Impact of the Entomopathogenic Fungus Conidiobolus coronatus on the Free Fatty Acid Profile of the Flesh Fly Sarcophaga argyrostoma
Authors: Kaczmarek Agata , Boguś Mieczysława Irena
Published: October 2021

The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus Conidiobolus coronatus. In this study, Sarcophaga argyrostoma flies were exposed to sporulating C. coronatus colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with C. coronatus exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi.
The Primary Complete Mitochondrial Genome of the Lappet Moth Brahmophthalma hearseyi (Lepidoptera: Brahmaeidae) and Related Phylogenetic Analysis
Authors: Yang Shan , Gao Shangren , Cai Shiyu , Zou Zhiwen , Xin Tianrong , Xia Bin
Published: October 2021

Background: Brahmophthalma hearseyi (Lepidoptera: Brahmaeidae) is widely distributed across China. Its larvae damage the leaves of many plants such as those belonging to the Oleaceae family, causing significant economic losses and seriously affecting the survival and reproduction of Cervus nippon; however, genetic data for this species are scarce. Methods: The complete mitochondrial genome (mitogenome) of B. hearseyi was sequenced using long-PCR and primer-walking methods. Phylogenetic analysis that was based on 13 PCGs and two rRNAs was carried out using the neighbor-joining and Bayesian interference methods. Results: The mitogenome is a typical circular molecule that is made up of 15,442 bp, which includes 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and an A + T-rich region (456 bp). All of the PCGs, except for COX1 and COX2, start with ATN codons. COX2 and ND5 use the incomplete termination codon T, and 11 other PCGs use the typical stop codon TAA. All tRNA genes, except for trnS1 and trnS2, display a typical cloverleaf structure; trnS1 lacks the “DHU” arm, whereas trnS2 exhibits two mismatched base pairs in the anticodon stem. Phylogenetic analysis showed that B. hearseyi is clustered into Brahmaeidae, and the phylogenetic relationships are (Brahmaeidae + Lasiocampidae) + (Bombycidae + (Sphingidae + Saturniidae)). Conclusions: This study provides the first mitogenomic resources for the Brahmaeidae.
Landmark Data to Distinguish and Identify Morphologically Close Tabanus spp. (Diptera: Tabanidae)
Authors: Changbunjong Tanasak , Prakaikowit Nutnicha , Maneephan Photchanun , Kaewwiset Tipparat , Weluwanarak Thekhawet , Chaiphongpachara Tanawat , Dujardin Jean-Pierre
Published: October 2021

Tabanus spp., also known as horse flies (Diptera: Tabanidae), are important vectors of several animal pathogens. Adult females of Tabanus megalops and Tabanus striatus, which are members of the T. striatus complex, are morphologically similar and hence difficult to distinguish using morphological characteristics. In addition, molecular identification by DNA barcoding is also unable to distinguish these species. These two species can occur sympatrically with Tabanus rubidus, which is morphologically similar to T. megalops and T. striatus. Wing geometric morphometrics has been widely used in various insects to distinguish morphologically similar species. This study explored the effectiveness of landmark-based geometrics at distinguishing and identifying T. megalops, T. rubidus, and T. striatus in Thailand. Specimens were collected from different geographical regions of Thailand, and only unambiguously identified specimens were used for geometric morphometric analyses. Left wings of females of T. megalops (n = 160), T. rubidus (n = 165), and T. striatus (n = 85) were photographed, and 22 wing landmarks were used for the analysis. Wing shape was able to distinguish among species with high accuracy scores, ranging from 94.38% to 99.39%. We showed that morphologically very close species of Tabanus can be reliably distinguished by the geometry of their wing venation, and we showed how our experimental material could be used as a reference to tentatively identify new field collected specimens.
Impact of Electroosmosis and Wall Properties in Modelling Peristaltic Mechanism of a Jeffrey Liquid through a Microchannel with Variable Fluid Properties
Authors: Rajashekhar Choudhari , Mebarek-Oudina Fateh , Sarris Ioannis E. , Vaidya Hanumesh , Prasad Kerehalli V. , Manjunatha Gudekote , Balachandra Hadimane
Published: October 2021

The current work emphasizes the modelling of the electroosmosis-modulated peristaltic flow of Jeffery liquid. Such flows emerge in understanding the movement of biological fluids in a microchannel, such as in targeted drug delivery and blood flow through micro arteries. The non-Newtonian fluid flows inside a non-uniform cross-section and an inclined microchannel. The effects of wall properties and variable fluid properties are considered. The long wavelength and small Re number approximations are assumed to simplify the governing equations. Debye-Hückel linearization is also utilized. The nonlinear governing equations are solved by utilizing the perturbation technique. MATLAB is used for the solution, velocity, temperature, skin friction, coefficient heat transport, concentration, shear wood number, and streamlines expressions. The obtained result in optimal electroosmotic velocity (or Helmholtz-Smoluchowski velocity) increases from −1 to 6; the axial circulation has substantial momentum. For larger optimal electroosmotic velocity, a subsequent boost in an axial electric field causes a significant deceleration. Further, the study helps biomedical engineers to create biomicrofluidics devices that may aid in carrying biological fluids.
Elevated Glucagon-like Peptide-1 and a Th2 Shift May Support Reduced Prevalence of Thoracic Aortic Aneurysm in Patients with Diabetes
Authors: Ntika Stelia , Jois Harshitha , Lång Karin , Olsson Christian , Franco-Cereceda Anders , Björck Hanna M. , Krizhanovskii Camilla
Published: October 2021

Glucagon-like peptide-1 (GLP-1) regulates processes involved in the pathophysiology of thoracic aortic aneurysms (TAAs), including inflammation, while protecting against aortic aneurysms in animal models. Type 2 diabetes (T2D) involves altered GLP-1 signaling due to pathology and/or therapy and is associated with reduced prevalence of TAAs. We aimed to assess whether T2D alters the inflammatory profile/proteolytic activity, possible correlations to elevated fasting GLP-1 (F-GLP-1), and its relevance for TAA. F-GLP-1, pro-inflammatory T helper 1 (Th1) cytokines, Th2 cytokines, C-reactive protein, and matrix metalloproteinase-2 activity (MMP-2) were analyzed in surgical patients with aortic valve pathology with/without T2D and without T2D but with TAA. Patients with T2D displayed an increase in the relative systemic expression of interleukin 6 and tumor necrosis factor α and a clear trend towards reduced levels of interferon γ (IFNγ). In addition, a positive association between GLP-1 and the plasma interleukin 4 (IL-4)/IFNγ ratio was detected. TAA was associated with significantly lower plasma levels of the Th2 cytokines IL-4 and interleukin 5. Plasma MMP-2 activity did not differ between groups. We conclude that T2D involved a Th2 shift, which associates with elevated F-GLP-1 and may—considering Th1 bias in TAA—contribute to reduced prevalence of TAA in T2D.
Micro-Elimination: Updated Pathway to Global Elimination of Hepatitis C in Small Communities and Industrial Settings during the COVID 19 Pandemic
Authors: Butaru Anca Elena , Gheonea Dan Ionuț , Rogoveanu Ion , Diculescu Mircea , Boicea Ancuța-Ramona , Bunescu Marius , Streba Costin-Teodor , Oancea Carmen Nicoleta
Published: October 2021

Background: In response to the goal of the World Health Organisation to eliminate hepatitis C virus infections by 2030, Romania is striving for national elimination. An already successful micro-elimination project was expanded to test-and-treat specific populations and at-risk groups. The aim of this project was to identify the individuals with HCV infection in disadvantaged regions who do not have proper medical care access. Materials and Methods: Our two-arm interventional cross-sectional study used rapid anti-HCV antibody testing on two population groups from the Romanian southwestern region of Oltenia, approached between September 2020 and May 2021. The first group consisted of predominantly over 40 years old individuals, recruited through five family doctors from two medium-sized towns (community lot—CL). We approached a second group, aged 18–65, through 11 medical offices of five large factories in the same region (industry lot, IL). A 12-items questionnaire was given to each participant, to determine risk factors and record demographic data. Eligible patients initiated antiviral therapy using direct-acting antivirals (DAAs). Results: We enrolled 15,383 individuals between all 16 locations. The overall prevalence by antibody testing was 0.77% (119 cases). Of these, 57 subsequently received treatment with DAAs. We identified blood transfusions as a risk factor within the CL. Participants in the IL reported a relatively high risk for the following situations: sharing of personal hygiene belongings with another person, performing previous blood transfusions, dental interventions and previous surgery. Conclusions: In this global context, the use of micro-elimination allows interventions to be faster and more efficient. This is possible by targeting smaller and specific HCV risk groups.
Carotid Artery Temperature Reduction with Statin Therapy in Patients with Familial Hyperlipidemia Syndromes
Authors: Benetos Georgios , Galanakos Spyros , Koutagiar Iosif , Skoumas Ioannis , Oikonomou Georgios , Drakopoulou Maria , Karmpalioti Maria , Katsi Vasiliki , Tsioufis Costas , Toutouzas Konstantinos
Published: October 2021

Background: Microwave radiometry (MWR) assesses non-invasive carotid artery temperatures reflecting inflammation. In the present study, we aimed to investigate the impact of hypolipidemic therapy either with simvastatin or with combination simvastatin plus ezetimibe on carotid artery temperatures of patients with familial hyperlipidemia syndromes (FHS). Methods: Consecutive patients with diagnosis of either familial heterozygous hypercholesterolemia (heFH) or familial combined hyperlipidemia (FCH) were included in the study. Patients were assigned to either simvastatin 40 mg or simvastatin 40 mg plus ezetimibe 10 mg, according to the discretion of the physician. FHS patients who refused statin therapy were used as a control group. Common carotid intima-media thickness (ccIMT) was measured and ΔΤ (maximum-minimum) temperature measurements were performed across each carotid during MWR evaluation. RESULTS: In total, 115 patients were included in the study. Of them, 40 patients received simvastatin (19 heFH and 21 FCH), 41 simvastatin + ezetimibe (31 heFH and 10 FCH), and 34 (21 heFH and 13 FCH) no statin. Carotid artery temperatures were significantly reduced at 6 months in FH patients who received hypolipidemic treatment (0.83 ± 0.34 versus 0.63 ± 0.24 °C, p = 0.004 for simvastatin, 1.00 ± 0.38 versus 0.69 ± 0.23 °C, p < 0.001 for simvastatin + ezetimibe), but no change was recorded in controls (0.72 ± 0.26 versus 0.70 ± 0.26 °C, p = 0.86). Conclusions: Hypolipidemic therapy reduced carotid temperatures in FHS patients.
Low-Frequency Magnetoelectric Effects in Magnetostrictive–Piezoelectric Bilayers: Longitudinal and Bending Deformations
Authors: Filippov Dmitry , Liu Ying , Zhou Peng , Ge Bingfeng , Liu Jiahui , Zhang Jitao , Zhang Tianjin , Srinivasan Gopalan
Published: October 2021

A model for the low-frequency magnetoelectric (ME) effect that takes into consideration the bending deformation in a ferromagnetic and ferroelectric bilayer is presented. Past models, in general, ignored the influence of bending deformation. Based on the solution of the equations of the elastic theory and electrostatics, expressions for the ME voltage coefficients (MEVCs) and ME sensitivity coefficients (MESCs) in terms of the physical parameters of the materials and the geometric characteristic of the structure were obtained. Contributions from both bending and planar deformations were considered. The theory was applied to composites of PZT and Ni with negative magnetostriction, and Permendur, or Metglas, both with positive magnetostriction. Estimates of MEVCs and MESCs indicate that the contribution from bending deformation is significant but smaller than the contribution from planar deformations, leading to a reduction in the net ME coefficients in all the three bilayer systems.
Functionalized Antimicrobial Nanofibers: Design Criteria and Recent Advances
Authors: Hamdan Nazirah , Yamin Alisa , Hamid Shafida Abd , Khodir Wan Khartini Wan Abdul , Guarino Vincenzo
Published: October 2021

The rise of antibiotic resistance has become a major threat to human health and it is spreading globally. It can cause common infectious diseases to be difficult to treat and leads to higher medical costs and increased mortality. Hence, multifunctional polymeric nanofibers with distinctive structures and unique physiochemical properties have emerged as a neo-tool to target biofilm and overcome deadly bacterial infections. This review emphasizes electrospun nanofibers’ design criteria and properties that can be utilized to enhance their therapeutic activity for antimicrobial therapy. Also, we present recent progress in designing the surface functionalization of antimicrobial nanofibers with non-antibiotic agents for effective antibacterial therapy. Lastly, we discuss the future trends and remaining challenges for polymeric nanofibers.
Greenhouse Gas Emission Model for Tidal Flats in the Republic of Korea
Authors: Dang Nhi Yen Thi , Park Heung-Sik , Mir Kaleem Anwar , Kim Choong-Gon , Kim Seungdo
Published: October 2021

Since coastal wetlands have been severely degraded and polluted by human activities, they have increasingly become a significant source of greenhouse gases (GHGs), so understanding the characteristics of their emissions is critical for devising future climate change mitigation strategies. This study modified a model based on carbon balance to forecast carbon stored and CO2, CH4 emissions in four types of typical tidal flats—Phragmites australis (PA), Spartina alterniflora (SA), Suaeda japonica (SJ), and Bare Tidal Flat (BTF) in Korea’s Ganghwa province from 2017 to 2047. The model was built using biomass data from salt plant species collected in different locations. The results indicate that the total annual simulated flow of CH4 increased over time in all four areas, most notably in SA, while CO2 remained relatively stable. The mean CO2 and CH4 fluxes in the four types of representative tidal flats were in the range of 0.03 to 19.1 mg m−2 d−1 and 0.007 to 5.23 mg m−2 d−1, respectively, across all seasons. Besides, the results indicate that the amount of carbon accumulated in the top soil increases linearly over time in nearly all areas studied, ranging from 0.01 to 0.13 (kgC m−2 yr−1). In general, the study provides a model for Korean tidal flats that incorporates carbon storage and GHG emissions in the intertidal zone in order to develop potential GHG reduction scenarios.
Assessment of Inoculation Methods of Thielaviopsis paradoxa (De Seynes) Höhn into Oil Palm Seedlings under Greenhouse Conditions
Authors: Gaitán-Chaparro Sandra , Navia-Rodríguez Edwin , Romero Hernán Mauricio
Published: October 2021

Oil palm (Elaeis guineensis Jacq. and Elaeis Oleifera Cortes) is one of the most important oil crops in the world. Colombia is the fourth-largest oil palm producer worldwide. However, oil palm diseases are a significant factor affecting yield. Thielaviopsis paradoxa (De Seynes) Höhn is a pathogen that affects young palm trees, causing spear rot. Four disease establishment methods were studied to replicate, in a controlled environment, the symptoms of the disease found in the field. Young palm trees were inoculated with a suspension of endoconidia using either local infiltration, drip, scissor cut, or direct contact with agar blocks bearing mycelia and conidia. The effects of the inoculation methods were studied in dose-method-disease severity experiments conducted in a greenhouse under controlled conditions. All four methods resulted in T. paradoxa infections and the development of symptoms of the disease. The disease severity was correlated with the method and dose of inoculation. In trials to test Koch’s postulates, T. paradoxa was isolated from areas of disease progression in the inoculated trees, but the teleomorph Ceratocystis paradoxa (Dade) Moreau was not observed. A photographic record of the infection process at different times post-infection was compiled. Given that establishing the disease through artificial inoculation is essential for assessing plant pathogenesis, this study determined that the local infiltration method (1 × 106 endoconidia mL−1) and a 3–7 day incubation period were critical for the development of symptoms as severe as those observed in natural infections in the field.
Pre-Hispanic Foods Oyster Mushroom (Pleurotus ostreatus), Nopal (Opuntia ficus-indica) and Amaranth (Amaranthus sp.) as New Alternative Ingredients for Developing Functional Cookies
Authors: Uriarte-Frías Georgina , Hernández-Ortega Martha M. , Gutiérrez-Salmeán Gabriela , Santiago-Ortiz Miriam Magale , Morris-Quevedo Humberto J. , Meneses-Mayo Marcos
Published: October 2021

Oyster mushroom (Pleurotusostreatus), nopal (Opuntia ficus-indica) and amaranth (Amaranthus spp.) are pre-Hispanic foods widely consumed in Mexico. However, there are no standard products developed with these ingredientsas functional cookies. This study evaluated the impact of partial replacement (50%) of whole-wheat flour (WWF) with three formulations of P. ostreatus, nopal and amaranth flours (POF, NF and AF, respectively) on the nutritional/antioxidant properties of fortified cookies. The proportion of the flours’ ingredients (WWF:AF:NF:POF) were 100% WWF (traditional cookies), 50:35:10:5 (F1), 50:30:15:5 (F2) and 50:40:5:5 (F3). Proximal composition, phenolic/flavonoid contents, and ABTS•+ scavenging activity were determined in flours and cookies.POF, NF and AF possess a high nutritional value comprising polyphenols/flavonoids and a significant antioxidant potential. Total protein, ash and flavonoids were higher in fortified cookies than in controls. Cookies prepared with F2—the highest nopal level—contained 5.29% of dietary fiber and five times higher polyphenol content than control cookies. The ABTS•+ scavenging ability was similar in the three enriched cookies (87.73–89.58%), but higher than that in traditional cookies (75.60%). The applicability of POF/NF/AF for replacing up to 50% of WWF in the production of functional cookies was demonstrated without compromising products’ acceptability.This research promotes renewable local bioresouces for a sustainable agri-food chain, especially edible mushrooms.
Novel Antioxidants and α-Glycosidase and Protein Tyrosine Phosphatase 1B Inhibitors from an Endophytic Fungus Penicillium brefeldianum F4a
Authors: Bai Yan , Yi Ping , Zhang Songya , Hu Jiangchun , Pan Huaqi
Published: October 2021

Oxidative stress plays a very important role in the progression of diabetes and its complications. A therapeutic agent that is both antidiabetic and antioxidant would be the preferred choice for the treatment of diabetes. The crude extract of the endophytic fungus Penicillium brefeldianum F4a has significant antioxidant and α-glycosidase and protein tyrosine phosphatase 1B (PTP1B) inhibition activities. Chemical investigation of P. brefeldianum F4a using an activity-guided isolation led to the discovery of three new compounds called peniorcinols A–C (1–3) along with six known compounds: penialidins A (4), penialidin F (5), myxotrichin C (6), riboflavin (7), indole-3-acetic acid (8), and 2-(4-hydroxy-2-methoxy-6-methylphenyl) acetic acid (9). Their chemical structures were established by their NMR and HRESIMS. The absolute configurations of 1 and 3 were determined by experimental and calculated electronic circular dichroism (ECD). Their antioxidant activities were evaluated by DPPH• and ABTS•+ scavenging assays. Compounds 1–6 and 8–9 showed moderate to strong free radical scavenging activities. Significantly, 4–6 exhibited more potent ABTS•+ scavenging activity than that of the positive control. Their α-glycosidase and PTP1B inhibition activities were tested. Among them, compound 3 showed α-glucosidase inhibition activity, and compounds 7 and 8 showed PTP1B inhibitory activity for the first time. It is worth noting that 3 and 8 displayed both antioxidant and α-glycosidase or PTP1B inhibition activities. These finding suggest that compounds 3 and 8 could be used as lead compounds to generate new potent drugs for the treatment of oxidative stress-related diabetes.
Improved Mineral Acquisition, Sugars Metabolism and Redox Status after Mycorrhizal Inoculation Are the Basis for Tolerance to Vanadium Stress in C3 and C4 Grasses
Authors: Selim Samy , Abuelsoud Walid , Alsharari Salam S. , Alowaiesh Bassam F , Al-Sanea Mohammad M. , Al Jaouni Soad , Madany Mahmoud M. Y. , AbdElgawad Hamada
Published: October 2021

Vanadium (V) can be beneficial or toxic to plant growth and the interaction between arbuscular mycorrhizal fungi (AMF) and V stress was rarely investigated at physiological and biochemical levels of plant groups (C3 and C4) and organs (roots and shoots). We tested the potential of AMF to alleviate the negative effects of V (350 mg V/Kg soil) on shoots and roots of rye and sorghum. Relative to sorghum (C4), rye (C3) showed higher levels of V and lower levels of key elements under V stress conditions. V inhibited growth, photosynthesis, and induced photorespiration (increased HDR & GO activities) and oxidative damage in both plants. AMF colonization reduced V stress by differently mitigating the oxidative stress in rye and sorghum. This mitigation was accompanied with increases in acid and alkaline phosphatase activities in plant roots and increased organic acids and polyphenols exudation into the soil, thus reduced V accumulation (29% and 58% in rye and sorghum shoot, respectively) and improved absorption of mineral nutrients including Ca, Mg and P. AMF colonization improved photosynthesis and increased the sugar accumulation and metabolism. Sugars also acted as a supplier of C skeletons for producing of antioxidants metabolite such as ascorbate. At the antioxidant level, rye was more responsive to the mitigating impact of AMF. Higher antioxidants and detoxification defence system (MTC, GST, phenolics, tocopherols and activities of CAT, SOD and POX) was recorded for rye, while sorghum (C4) improved its GR activity. The C3/C4-specificity was supported by principal component analysis. Together, this study provided both fundamental and applied insights into practical strategies to mitigate the phytotoxicity hazards of V in C3 and C4 grasses. Moreover, our results emphasize the importance of AMF as an environment-friendly factor to alleviate stress effects on plants and to improve growth and yield of unstressed plants.
10th Trends in Medical Mycology Held on 8 to 11 October 2021, Aberdeen, Scotland, Organized by the European Confederation of Medical Mycology (ECMM)
Authors: Cornely Oliver A. , Gow Neil , Hoenigl Martin , Warris Adilia
Published: October 2021

Plenary Sessions: [...]
Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive Mycoparasite Calcarisporium cordycipiticola with Host Specificity
Authors: Liu Qing , Xu Yanyan , Zhang Xiaoling , Li Kuan , Li Xiao , Wang Fen , Xu Fangxu , Dong Caihong
Published: October 2021

Calcarisporium cordycipiticola is the pathogen in the white mildew disease of Cordyceps militaris, one of the popular mushrooms. This disease frequently occurs and there is no effective method for disease prevention and control. In the present study, C. militaris is found to be the only host of C. cordycipiticola, indicating strict host specificity. The infection process was monitored by fluorescent labeling and scanning and transmission electron microscopes. C. cordycipiticola can invade into the gaps among hyphae of the fruiting bodies of the host and fill them gradually. It can degrade the hyphae of the host by both direct contact and noncontact. The parasitism is initially biotrophic, and then necrotrophic as mycoparasitic interaction progresses. The approximate chromosome-level genome assembly of C. cordycipiticola yielded an N50 length of 5.45 Mbp and a total size of 34.51 Mbp, encoding 10,443 proteins. Phylogenomic analysis revealed that C. cordycipiticola is phylogenetically close to its specific host, C. militaris. A comparative genomic analysis showed that the number of CAZymes of C. cordycipiticola was much less than in other mycoparasites, which might be attributed to its host specificity. Secondary metabolite cluster analysis disclosed the great biosynthetic capabilities and potential mycotoxin production capability. This study provides insights into the potential pathogenesis and interaction between mycoparasite and its host.
Social Welfare Analysis under Different Levels of Consumers’ Privacy Regulation
Authors: Lin Xudong , Huang Xiaoli , Liu Shuilin , Li Yulin , Luo Hanyang , Yu Sumin
Published: October 2021

With the rapid development of information technology, digital platforms can collect, utilize, and share large amounts of specific information of consumers. However, these behaviors may endanger information security, thus causing privacy concerns among consumers. Considering the information sharing among firms, this paper constructs a two-period duopoly price competition Hotelling model, and gives insight into the impact of three different levels of privacy regulations on industry profit, consumer surplus, and social welfare. The results show that strong privacy protection does not necessarily make consumers better off, and weak privacy protection does not necessarily hurt consumers. Information sharing among firms will lead to strong competitive effects, which will prompt firms to lower the price for new customers, thus damaging the profits of firms, and making consumers’ surplus higher. The level of social welfare under different privacy regulations depends on consumers’ product-privacy preference, and the cost of information coordination among firms. With the cost of information coordination among firms increasing, it is only in areas where consumers have greater privacy preferences that social welfare may be optimal under the weak regulation.
Including Condition into Ecological Maps Changes Everything—A Study of Ecological Condition in the Conterminous United States
Authors: Knight Kevin B. , Comer Patrick J. , Pickard Brian R. , Gordon Doria R. , Toombs Theodore
Published: October 2021

In 2021, the Biden administration signed an executive order to protect 30% of American lands by 2030. Accomplishing this ambitious goal in the U.S. requires understanding the relative contribution of public and private lands toward supporting biodiversity. New approaches are needed because existing approaches focus on quantity of habitat without incorporating quality. To fill this need, we developed a 30 m resolution national habitat condition index (HCI) that integrates quality and quantity measures of habitat. We hypothesized that including an evaluation of the quality of habitat at landscape scales, both in conservation-focused preserves and working lands would provide a better assessment of the value of geographies for conservation. We divided the conterminous U.S. by major land cover type and into natural and cultivated lands and then spatially mapped multiple anthropogenic stressors, proximity to aquatic habitat, and vegetation departure from expected natural disturbance regimes. Each map layer was then scored for site impact and distance decay and combined into a final national index. Field observations providing scored relative ecological conditions were used for HCI calibration and validation at both CONUS and regional scales. Finally, we evaluate lands by management (conservation versus working lands) and ownership (public versus private) testing the value of these lands for conservation. While we found regional differences across CONUS, functional habitat was largely independent of protection status: working lands provide clear habitat and other values. These results are relevant for guiding strategies to achieve the U.S. 30 by 30 goals. Where similar data exist in other countries, analogous modeling could be used to meet their national conservation commitments.
Highly Conducting Li(Fe1−xMnx)0.88V0.08PO4 Cathode Materials Nanocrystallized from the Glassy State (x = 0.25, 0.5, 0.75)
Authors: Frąckiewicz Justyna E. , Pietrzak Tomasz K.
Published: October 2021

This study showed that thermal nanocrystallization of glassy analogs of LiFe1−xMnxPO4 (with the addition of vanadium for improvement of glass forming properties) resulted in highly conducting materials that may be used as cathodes for Li-ion batteries. The glasses and nanomaterials were studied with differential thermal analysis, X-ray diffractometry, and impedance spectroscopy. The electrical conductivity of the nanocrystalline samples varied, depending on the composition. For x=0.5, it exceeded 10−3 S/cm at room temperature with an activation energy as low as 0.15 eV. The giant and irreversible increase in the conductivity was explained on the basis of Mott’s theory of electron hopping and a core-shell concept. Electrochemical performance of the active material with x=0.5 was also reported.