bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Dataset S5 from Therapeutic Targeting of the Premetastatic Stage in Human Lung-to-Brain Metastasis
Authors: Singh Mohini , Venugopal Chitra , Tokar Tomas , McFarlane Nicole , Subapanditha Minomi K. , Qazi Maleeha , Bakhshinyan David , Vora Parvez , Murty Naresh K. , Jurisica Igor , Singh Sheila K.
Published: April 2023

Heatmap of dysregulated BMIT pathways
Figure S5 from Cancer-Associated Point Mutations in the DLC1 Tumor Suppressor and Other Rho-GAPs Occur Frequently and Are Associated with Decreased Function
Authors: Wang Dunrui , Qian Xiaolan , Sanchez-Solana Beatriz , Tripathi Brajendra K. , Durkin Marian E. , Lowy Douglas R.
Published: April 2023

The Rho-GAP activity of DLC1 mutants and focal adhesions
Figure S4 from Cancer-Associated Point Mutations in the DLC1 Tumor Suppressor and Other Rho-GAPs Occur Frequently and Are Associated with Decreased Function
Authors: Wang Dunrui , Qian Xiaolan , Sanchez-Solana Beatriz , Tripathi Brajendra K. , Durkin Marian E. , Lowy Douglas R.
Published: April 2023

DLC1 mRNA in colon cancer cell lines
Supplementary Figure 1 from Structural Optimization and Enhanced Prodrug-Mediated Delivery Overcomes Camptothecin Resistance in High-Risk Solid Tumors
Authors: Nguyen Ferro , Guan Peng , Guerrero David T. , Kolla Venkatadri , Naraparaju Koumudi , Perry Lauren M. , Soberman Danielle , Pressly Benjamin B. , Alferiev Ivan S. , Chorny Michael , Brodeur Garrett M.
Published: April 2023

Blood counts
Table S6 from A Circle RNA Regulatory Axis Promotes Lung Squamous Metastasis via CDR1-Mediated Regulation of Golgi Trafficking
Authors: Harrison Emily B. , Porrello Alessandro , Bowman Brittany M. , Belanger Adam R. , Yacovone Gabriella , Azam Salma H. , Windham Ian A. , Ghosh Subrata K. , Wang Menglin , Mckenzie Nicholas , Waugh Trent A. , Van Swearingen Amanda E.D. , Cohen Stephanie M. , Allen Devon G. , Goodwin Tyler J. , Mascenik Teresa , Bear James E. , Cohen Sarah , Randell Scott H. , Massion Pierre P. , Major Michael B. , Huang Leaf , Pecot Chad V.
Published: April 2023

Gene ontology terms associated with genes elevated in High CDR1-Low miR-671-5p tumors compared to Low CDR1-High miR-671 tumors in LUSC TCGA.
Supplementary Tables from Dynamics of Genomic, Epigenomic, and Transcriptomic Aberrations during Stepwise Hepatocarcinogenesis
Authors: Jee Byul A , Choi Ji-Hye , Rhee Hyungjin , Yoon Sarah , Kwon So Mee , Nahm Ji Hae , Yoo Jeong Eun , Jeon Youngsic , Choi Gi Hong , Woo Hyun Goo , Park Young Nyun
Published: April 2023

Supplementary Table S1: Tissue specimens used for RNA-seq analysis. Supplementary Table S2: Tissues specimens used for immunohistochemical analysis. Supplementary Table S3: Gene signatures used in the study. Supplementary Table S4: Differentially methylated probes during hepatocarcinogenesis. Supplementary Table S5: Univariate and multivariate analysis for SPINK1 expression and HCC clinico-pathological features in TCGA-LIHC data.
Supplementary Figures from Dynamics of Genomic, Epigenomic, and Transcriptomic Aberrations during Stepwise Hepatocarcinogenesis
Authors: Jee Byul A , Choi Ji-Hye , Rhee Hyungjin , Yoon Sarah , Kwon So Mee , Nahm Ji Hae , Yoo Jeong Eun , Jeon Youngsic , Choi Gi Hong , Woo Hyun Goo , Park Young Nyun
Published: April 2023

Supplementary Figure S1: Overall study design for multi-omic data analysis. Supplementary Figure S2: The distribution of beta-values in DNA methylation data. Supplementary Figure S3: The distribution of differentially methylated probes (DMPs). Supplementary Figure S4: Differentially methylated probes in the integrated data of GSE44970 and YSHCC. Supplementary Figure S5: Distribution of CNAs during stepwise hepatocarcinogenesis. Supplementary Figure S6: DNA methylation of oncogenes and TSGs during multi-step hepatocarcinogenesis. Supplementary Figure S7: Frequencies of nonsynonymous or missense mutations in each patient. Supplementary Figure S8: Functionally enriched expression in each step of hepatocarcinogenesis. Supplementary Figure S9: Validation of the three genes including SPINK1, CAP2, and RRAGD. Supplementary Figure S10: SPINK1 overexpression increases proliferation and invasion of Huh7 cells. Supplementary Figure S11: Correlation between methylation and expression. Supplementary Figure S12: Western blot of DNMT1 expression. Supplementary Figure S13: GRP78 expression under ER stress. Supplementary Figure S14: Effects of knockdown of p65 expression in the expression of GRP78 and SPINK1.
Table S4 from A Deep Learning Framework Identifies Pathogenic Noncoding Somatic Mutations from Personal Prostate Cancer Genomes
Authors: Wang Cheng , Li Jingjing
Published: April 2023

Identification of significant regulatory somatic mutations in localized prostate cancer.
Table S1 from Flightless-I Blocks p62-Mediated Recognition of LC3 to Impede Selective Autophagy and Promote Breast Cancer Progression
Authors: He Jian-Ping , Hou Pei-Pei , Chen Qi-Tao , Wang Wei-Jia , Sun Xiao-Yu , Yang Peng-Bo , Li Ying-Ping , Yao Lu-Ming , Li Xiaotong , Jiang Xin-Dong , Chien Kun-Yi , Zhang Zhi-Ming , Wu Qiu-Wan , Cowin Allison J. , Wu Qiao , Chen Hang-Zi
Published: April 2023

Clinical information of patients with breast cancer.
Table S1 from A Deep Learning Framework Identifies Pathogenic Noncoding Somatic Mutations from Personal Prostate Cancer Genomes
Authors: Wang Cheng , Li Jingjing
Published: April 2023

Information about clinical samples and the list of simple somatic mutations from the ICGC PRAD-CA cohort.
Supplementary Data from Local Targeting of NAD+ Salvage Pathway Alters the Immune Tumor Microenvironment and Enhances Checkpoint Immunotherapy in Glioblastoma
Authors: Li Ming , Kirtane Ameya R. , Kiyokawa Juri , Nagashima Hiroaki , Lopes Aaron , Tirmizi Zain A. , Lee Christine K. , Traverso Giovanni , Cahill Daniel P. , Wakimoto Hiroaki
Published: April 2023

Supplemental figures 1-10. Supplementary Figure S1. Cell viability assay with NAMPT inhibitors. Supplementary Figure S2. NAMPT inhibitor induces PD-L1 upregulation on alive cells. Supplementary Figure S3. NAMPT inhibitor induces PD-L1 upregulation. Supplementary Figure S4. Tumor PD-L1 upregulation after injection of coumarin6GMX1778 co-loaded microparticles. Supplementary Figure S5. NAMPT inhibitor-induced autophagy underlies an increase in PD-L1 mRNA and protein levels. Supplementary Figure S6. Impact of local treatment with GMX1778 microparticles on cells labeled with Arg1 and CD68 in murine glioblastoma. Supplementary Figure S7. NAMPT inhibitor decreases glioblastoma-associated macrophages. Supplementary Figure S8. PD-L1 immunohistochemistry (brown) of GL261 glioblastoma after treatment with blank micro-particles (MP), blank MP and anti-PD-1, GMX1778 MP, and combination of GMX1778 MP and anti-PD-1. Supplementary Figure S9. Immunoflurescence of GL261 glioblastoma after treatment with blank micro-particles, anti-PD-1, GMX1778 micro-particles, and combination. Supplementary Figure S10. GranzymeB immunohistochemistry (brown) of GL261 glioblastoma after treatment with blank micro-particles (MP), blank MP and anti-PD-1, GMX1778 MP, and combination of GMX1778 MP and anti-PD-1.
Supplementary Data 5 from Integrating Mathematical Modeling with High-Throughput Imaging Explains How Polyploid Populations Behave in Nutrient-Sparse Environments
Authors: Kimmel Gregory J. , Dane Mark , Heiser Laura M. , Altrock Philipp M. , Andor Noemi
Published: April 2023

Simulation shown in Fig. 4E
Figure S2 from Fibroblast-Derived IL33 Facilitates Breast Cancer Metastasis by Modifying the Immune Microenvironment and Driving Type 2 Immunity
Authors: Shani Ophir , Vorobyov Tatiana , Monteran Lea , Lavie Dor , Cohen Noam , Raz Yael , Tsarfaty Galia , Avivi Camila , Barshack Iris , Erez Neta
Published: April 2023

Figure S2 complements Figure 2 in showing that lung metastases formation is characterized by extensive modifications in the immune milieu.
Supplementary Table 2 from Brain Metastasis Cell Lines Panel: A Public Resource of Organotropic Cell Lines
Authors: Valiente Manuel , Van Swearingen Amanda E.D. , Anders Carey K. , Bairoch Amos , Boire Adrienne , Bos Paula D. , Cittelly Diana M. , Erez Neta , Ferraro Gino B. , Fukumura Dai , Gril Brunilde , Herlyn Meenhard , Holmen Sheri L. , Jain Rakesh K. , Joyce Johanna A. , Lorger Mihaela , Massague Joan , Neman Josh , Sibson Nicola R. , Steeg Patricia S. , Thorsen Frits , Young Leonie S. , Varešlija Damir , Vultur Adina , Weis-Garcia Frances , Winkler Frank
Published: April 2023

Contains experimental therapies tested on brain metastatic cell lines and associated clinical trials
Supplementary Data from Targeting Pan-ETS Factors Inhibits Melanoma Progression
Authors: Huang Lee , Zhai Yougang , La Jennifer , Lui Jason W. , Moore Stephen P.G. , Little Elizabeth C. , Xiao Sixia , Haresi Adil J. , Brem Candice , Bhawan Jag , Lang Deborah
Published: April 2023

Supplementary data and methods
Supplemental Table ST2 from Linking Transcriptomic and Imaging Data Defines Features of a Favorable Tumor Immune Microenvironment and Identifies a Combination Biomarker for Primary Melanoma
Authors: Gartrell-Corrado Robyn D. , Chen Andrew X. , Rizk Emanuelle M. , Marks Douglas K. , Bogardus Margaret H. , Hart Thomas D. , Silverman Andrew M. , Bayan Claire-Audrey Y. , Finkel Grace G. , Barker Luke W. , Komatsubara Kimberly M. , Carvajal Richard D. , Horst Basil A. , Chang Rui , Monod Anthea , Rabadan Raul , Saenger Yvonne M.
Published: April 2023

Patient characteristics of the metastatic melanoma cohort.
Supplementary Figure S4 from Blocking Angiopoietin-2 Promotes Vascular Damage and Growth Inhibition in Mouse Tumors Treated with Small Doses of Radiation
Authors: Kallio Pauliina , Jokinen Elina , Högström Jenny , Das Suvendu , Heino Sarika , Lähde Marianne , Brodkin Jefim , Korhonen Emilia A. , Alitalo Kari
Published: April 2023

Supplementary Figure S4
Supplementary Figure S1 from Blocking Angiopoietin-2 Promotes Vascular Damage and Growth Inhibition in Mouse Tumors Treated with Small Doses of Radiation
Authors: Kallio Pauliina , Jokinen Elina , Högström Jenny , Das Suvendu , Heino Sarika , Lähde Marianne , Brodkin Jefim , Korhonen Emilia A. , Alitalo Kari
Published: April 2023

Supplementary Figure S1
Supplementary Material from CRISPR/Cas9-Mediated Point Mutation in Nkx3.1 Prolongs Protein Half-Life and Reverses Effects Nkx3.1 Allelic Loss
Authors: Bowen Cai , Shibata Maho , Zhang Hailan , Bergren Sarah K. , Shen Michael M. , Gelmann Edward P.
Published: April 2023

Supplementary Tables 1-4: Table 1. Primers for PCR amplification for CRISPR/Cas9 mouse models. Table 2. ssODN oligos. Table 3. Primers for genotyping of mouse mutation. Table 4. Antibodies used for immunofluorescence staining. Supplemental Figures 1-8: Supplementary Figure 1 A. Amino acid sequences of human (top) and murine (bottom) NKX3.1 showing consensus (central) sequence and location of serine 185 and 186 indicated by the box in the figure. B. Effect of S186A mutation on half-life of murine Nkx3.1. Supplementary Figure 2 A. Schematic of targeted sites for mutation at locus. Supplementary Fig 3 A. H&E staining of dorsolateral prostatic lobes at different time points. Results similar to those seen with anterior prostates are shown in the figure. Supplementary Fig 4 A. Immunohistochemistry for cytokeratins 5 and 8 in 2-month old anterior prostate lobes. The arrow in the section from Nkx3.1 show an area of early hyperplasia. The arrows in the section from Nkx3.1 show areas of epithelial detachment. Supplementary Figure 5 Nkx3.1 expression. Supplementary Fig 6 Cell proliferation. Supplementary Figure 7 TUNEL staining was done on paraffin sections from dorsolateral prostate lobes of Nkx3.1 , Nkx3.1 , and Nkx3.1 mice at 2, 6, and 10 months of age. Supplementary Figure 8 A. Cleaved caspase 3 staining was done on paraffin sections from both anterior and dorsolateral prostate lobes of Nkx3.1 , Nkx3.1 , and Nkx3.1 mice at 2, 6, and 10 months of age.
Suppl. Table 1 from Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors
Authors: Lopez Sambrooks Cecilia , Baro Marta , Quijano Amanda , Narayan Azeet , Cui Wei , Greninger Patricia , Egan Regina , Patel Abhijit , Benes Cyril H. , Saltzman W. Mark , Contessa Joseph N.
Published: April 2023

Results of the Lung Cancer Cell Line Screen for OST Inhibitor Sensitivity
Suppl Fig. 2 from Oligosaccharyltransferase Inhibition Overcomes Therapeutic Resistance to EGFR Tyrosine Kinase Inhibitors
Authors: Lopez Sambrooks Cecilia , Baro Marta , Quijano Amanda , Narayan Azeet , Cui Wei , Greninger Patricia , Egan Regina , Patel Abhijit , Benes Cyril H. , Saltzman W. Mark , Contessa Joseph N.
Published: April 2023

Characterization of the Osimertinib resistant H1975 cells.
Supplementary Figures from Slow-Cycling Cancer Stem Cells Regulate Progression and Chemoresistance in Colon Cancer
Authors: Shiokawa Daisuke , Sakai Hiroaki , Ohata Hirokazu , Miyazaki Toshiaki , Kanda Yusuke , Sekine Shigeki , Narushima Daichi , Hosokawa Masahito , Kato Mamoru , Suzuki Yutaka , Takeyama Haruko , Kambara Hideki , Nakagama Hitoshi , Okamoto Koji
Published: April 2023

Supplementary Figure 1-7
Supplementary Figure 2 from Cellular and Genetic Determinants of the Sensitivity of Cancer to α-Particle Irradiation
Authors: Yard Brian D. , Gopal Priyanka , Bannik Kristina , Siemeister Gerhard , Hagemann Urs B. , Abazeed Mohamed E.
Published: April 2023

Longer doubling times in cells most resistant to alpha-particle irradiation.
Supplementary Data from Epigenetic Inactivation of α-Internexin Accelerates Microtubule Polymerization in Colorectal Cancer
Authors: Li Yingjie , Bai Liangliang , Yu Huichuan , Cai Du , Wang Xiaolin , Huang Baoyuan , Peng Shaoyong , Huang Meijin , Cao Guangwen , Kaz Andrew M. , Grady William M. , Wang Jianping , Luo Yanxin
Published: April 2023

Supplemental Figures 1-10 and Reagent table
Fig. S1 from Hes1 Is Essential in Proliferating Ductal Cell–Mediated Development of Intrahepatic Cholangiocarcinoma
Authors: Matsumori Tomoaki , Kodama Yuzo , Takai Atsushi , Shiokawa Masahiro , Nishikawa Yoshihiro , Matsumoto Tomonori , Takeda Haruhiko , Marui Saiko , Okada Hirokazu , Hirano Tomonori , Kuwada Takeshi , Sogabe Yuko , Kakiuchi Nobuyuki , Tomono Teruko , Mima Atsushi , Morita Toshihiro , Ueda Tatsuki , Tsuda Motoyuki , Yamauchi Yuki , Kuriyama Katsutoshi , Sakuma Yojiro , Ota Yuji , Maruno Takahisa , Uza Norimitsu , Marusawa Hiroyuki , Kageyama Ryoichiro , Chiba Tsutomu , Seno Hiroshi
Published: April 2023

Comparison of Albcre; Hes1flox/flox and control mice in liver development.
Supplementary Data from Kras/ADAM17-Dependent Jag1-ICD Reverse Signaling Sustains Colorectal Cancer Progression and Chemoresistance
Authors: Pelullo Maria , Nardozza Francesca , Zema Sabrina , Quaranta Roberta , Nicoletti Carmine , Besharat Zein Mersini , Felli Maria Pia , Cerbelli Bruna , d'Amati Giulia , Palermo Rocco , Capalbo Carlo , Talora Claudio , Di Marcotullio Lucia , Giannini Giuseppe , Checquolo Saula , Screpanti Isabella , Bellavia Diana
Published: April 2023

The supplementary material file contains additional data that sustain the oncogenic role of Jag1-ICD and information about reagents utilized, as follows: - Supplementary Figure S1 recapitulates the main gene mutations in CRC cell lines . - Supplementary Figures S2 and S3 show additional data supporting the role of Jag1-ICD in sustaining EMT and chemoresistance of CRC, respectively. - Supplementary Tables add information concerning reagents utilized. Table S1 reports the oligonucleotide primers; Table S2 reports the names and the source of the antibodies.
Table S1 from Insights into the Links between MYC and 3D Chromatin Structure and Epigenetics Regulation: Implications for Cancer Therapy
Authors: Amjadi-Moheb Fatemeh , Paniri Alireza , Akhavan-Niaki Haleh
Published: April 2023

Non-coding RNAs involved in tumorigenesis by dysregulating the expression of MYC.
Supplementary Data from Mesothelin-Specific CAR T Cells Target Ovarian Cancer
Authors: Schoutrop Esther , El-Serafi Ibrahim , Poiret Thomas , Zhao Ying , Gultekin Okan , He Rui , Moyano-Galceran Lidia , Carlson Joseph W. , Lehti Kaisa , Hassan Moustapha , Magalhaes Isabelle , Mattsson Jonas
Published: April 2023

Supplementary material
Figure S8 from Alterations in the Global Proteome and Phosphoproteome in Third Generation EGFR TKI Resistance Reveal Drug Targets to Circumvent Resistance
Authors: Zhang Xu , Maity Tapan K. , Ross Karen E. , Qi Yue , Cultraro Constance M. , Bahta Meriam , Pitts Stephanie , Keswani Meghana , Gao Shaojian , Nguyen Khoa Dang P. , Cowart Julie , Kirkali Fatos , Wu Cathy , Guha Udayan
Published: April 2023

Supplementary Fig.S8
Figure S4 from Alterations in the Global Proteome and Phosphoproteome in Third Generation EGFR TKI Resistance Reveal Drug Targets to Circumvent Resistance
Authors: Zhang Xu , Maity Tapan K. , Ross Karen E. , Qi Yue , Cultraro Constance M. , Bahta Meriam , Pitts Stephanie , Keswani Meghana , Gao Shaojian , Nguyen Khoa Dang P. , Cowart Julie , Kirkali Fatos , Wu Cathy , Guha Udayan
Published: April 2023

Supplementary Fig.S4
Figure S1 from Alterations in the Global Proteome and Phosphoproteome in Third Generation EGFR TKI Resistance Reveal Drug Targets to Circumvent Resistance
Authors: Zhang Xu , Maity Tapan K. , Ross Karen E. , Qi Yue , Cultraro Constance M. , Bahta Meriam , Pitts Stephanie , Keswani Meghana , Gao Shaojian , Nguyen Khoa Dang P. , Cowart Julie , Kirkali Fatos , Wu Cathy , Guha Udayan
Published: April 2023

Supplementary Fig.S1
Supplementary Data from Epigenetic Therapies in Ovarian Cancer Alter Repetitive Element Expression in a TP53-Dependent Manner
Authors: McDonald James I. , Diab Noor , Arthofer Elisa , Hadley Melissa , Kanholm Tomas , Rentia Uzma , Gomez Stephanie , Yu Angela , Grundy Erin E. , Cox Olivia , Topper Michael J. , Xing Xiaoyun , Strissel Pamela L. , Strick Reiner , Wang Ting , Baylin Stephen B. , Chiappinelli Katherine B.
Published: April 2023

Supplementary Data from Epigenetic Therapies in Ovarian Cancer Alter Repetitive Element Expression in a TP53-Dependent Manner
Figure S1-S8 from LncRNA SAMMSON Mediates Adaptive Resistance to RAF Inhibition in BRAF-Mutant Melanoma Cells
Authors: Han Shujun , Yan Yuwei , Ren Yibo , Hu Yiming , Wang Yan , Chen Lei , Zhi Zhe , Zheng Yan , Shao Yongping , Liu Jiankang
Published: April 2023

Supplementary figure and figure legends S1-S8
Figure S8 from MYC- and MIZ1-Dependent Vesicular Transport of Double-Strand RNA Controls Immune Evasion in Pancreatic Ductal Adenocarcinoma
Authors: Krenz Bastian , Gebhardt-Wolf Anneli , Ade Carsten P. , Gaballa Abdallah , Roehrig Florian , Vendelova Emilia , Baluapuri Apoorva , Eilers Ursula , Gallant Peter , D'Artista Luana , Wiegering Armin , Gasteiger Georg , Rosenfeldt Mathias T. , Bauer Stefan , Zender Lars , Wolf Elmar , Eilers Martin
Published: April 2023

TBK1-dependent regulation of NF-kB-dependent gene expression.
Supplementary Data and Methods from ALDH1A1 Activity in Tumor-Initiating Cells Remodels Myeloid-Derived Suppressor Cells to Promote Breast Cancer Progression
Authors: Liu Cuicui , Qiang Jiankun , Deng Qiaodan , Xia Jie , Deng Lu , Zhou Lei , Wang Dong , He Xueyan , Liu Ying , Zhao Botao , Lv Jinhui , Yu Zuoren , Lei Qun-Ying , Shao Zhi-Ming , Zhang Xiao-Yong , Zhang Lixing , Liu Suling
Published: April 2023

Supplementary Methods, Figures and Figure legends
Figure S6 from MYC- and MIZ1-Dependent Vesicular Transport of Double-Strand RNA Controls Immune Evasion in Pancreatic Ductal Adenocarcinoma
Authors: Krenz Bastian , Gebhardt-Wolf Anneli , Ade Carsten P. , Gaballa Abdallah , Roehrig Florian , Vendelova Emilia , Baluapuri Apoorva , Eilers Ursula , Gallant Peter , D'Artista Luana , Wiegering Armin , Gasteiger Georg , Rosenfeldt Mathias T. , Bauer Stefan , Zender Lars , Wolf Elmar , Eilers Martin
Published: April 2023

Further characterization of dsRNA species.
Supplementary Data from A Spatial and Functional Interaction of a Heterotetramer Survivin–DNA-PKcs Complex in DNA Damage Response
Authors: Güllülü Ömer , Hehlgans Stephanie , Mayer Benjamin E. , Gößner Ines , Petraki Chrysi , Hoffmann Melanie , Dombrowsky Maximilian J. , Kunzmann Patrick , Hamacher Kay , Strebhardt Klaus , Fokas Emmanouil , Rödel Claus , Münch Christian , Rödel Franz
Published: April 2023

Supplementary file S1: Protein and phosphosite abundances and descriptions of findings displayed in Figure 5E and Figure 6; and the output of pathway analysis findings displayed in Figure 5F.
Supplementary Figure from Loss of Integrin-Linked Kinase Sensitizes Breast Cancer to SRC Inhibitors
Authors: Beetham Henry , Griffith Billie G.C. , Murina Olga , Loftus Alexander E.P. , Parry David A. , Temps Carolin , Culley Jayne , Muir Morwenna , Unciti-Broceta Asier , Sims Andrew H. , Byron Adam , Brunton Valerie G.
Published: April 2023

Supplementary Figure from Loss of Integrin-Linked Kinase Sensitizes Breast Cancer to SRC Inhibitors
Supplementary Data from Ubiquitin-Specific Protease 6 Functions as a Tumor Suppressor in Ewing Sarcoma through Immune Activation
Authors: Henrich Ian C. , Jain Kanika , Young Robert , Quick Laura , Lindsay Jarrett M. , Park Daniel H. , Oliveira Andre M. , Blobel Gerd A. , Chou Margaret M.
Published: April 2023

Supp Figure 1-18
Supplementary Table S6 from Mutant Idh2 Cooperates with a NUP98-HOXD13 Fusion to Induce Early Immature Thymocyte Precursor ALL
Authors: Goldberg Liat , Negi Vijay , Chung Yang Jo , Onozawa Masahiro , Zhu Yuelin J. , Walker Robert L. , Pierce Rachel , Patel Daxesh P. , Krausz Kristopher W. , Gonzalez Frank J. , Goodell Margaret A. , Rodriguez Benjamin A.T. , Meltzer Paul S. , Aplan Peter D.
Published: April 2023

Whole exome sequencing data with SNVs.
Supplementary Movie A from Platelet TLR4-ERK5 Axis Facilitates NET-Mediated Capturing of Circulating Tumor Cells and Distant Metastasis after Surgical Stress
Authors: Ren Jinghua , He Jiayi , Zhang Hongji , Xia Yujia , Hu Zhiwei , Loughran Patricia , Billiar Timothy , Huang Hai , Tsung Allan
Published: April 2023

Supplementary Movie A
Supplementary Figure 1 from Platelet TLR4-ERK5 Axis Facilitates NET-Mediated Capturing of Circulating Tumor Cells and Distant Metastasis after Surgical Stress
Authors: Ren Jinghua , He Jiayi , Zhang Hongji , Xia Yujia , Hu Zhiwei , Loughran Patricia , Billiar Timothy , Huang Hai , Tsung Allan
Published: April 2023

Hepatic I/R promotes aggregation of platelets with CT26 tumor cells and facilitates NET-mediated tumor cell entrapment.
Supplementary Figure legends from Hepatitis B–Induced IL8 Promotes Hepatocellular Carcinoma Venous Metastasis and Intrahepatic Treg Accumulation
Authors: Zhang Changlu , Gao Yanan , Du Chengzhi , Markowitz Geoffrey J. , Fu Jing , Zhang Zhenxing , Liu Chunliang , Qin Wenhao , Wang Hongyang , Wang Fan , Yang Pengyuan
Published: April 2023

Supplementary Figure legends shows Figure S1-S6 legends
Table S4 from ZIP9 Is a Druggable Determinant of Sex Differences in Melanoma
Authors: Aguirre-Portolés Cristina , Payne Riley , Trautz Aspen , Foskett J. Kevin , Natale Christopher A. , Seykora John T. , Ridky Todd W.
Published: April 2023

Primers used to study YAP-1 target genes
Supplementary Figure S3 from Hepatitis B–Induced IL8 Promotes Hepatocellular Carcinoma Venous Metastasis and Intrahepatic Treg Accumulation
Authors: Zhang Changlu , Gao Yanan , Du Chengzhi , Markowitz Geoffrey J. , Fu Jing , Zhang Zhenxing , Liu Chunliang , Qin Wenhao , Wang Hongyang , Wang Fan , Yang Pengyuan
Published: April 2023

Supplementary Figure S3 shows Construction of IL-8 and CXCR1 overexpressed stable cell lines
Supplementary Figure S1 from Hepatitis B–Induced IL8 Promotes Hepatocellular Carcinoma Venous Metastasis and Intrahepatic Treg Accumulation
Authors: Zhang Changlu , Gao Yanan , Du Chengzhi , Markowitz Geoffrey J. , Fu Jing , Zhang Zhenxing , Liu Chunliang , Qin Wenhao , Wang Hongyang , Wang Fan , Yang Pengyuan
Published: April 2023

Supplementary Figure S1 shows Correlation of IL-8 expression with overall survival in multiple cancers.
Supplementary Table 3 from Human Endogenous Retrovirus Type K Promotes Proliferation and Confers Sensitivity to Antiretroviral Drugs in Merlin-Negative Schwannoma and Meningioma
Authors: Maze Emmanuel A. , Agit Bora , Reeves Shona , Hilton David A. , Parkinson David B. , Laraba Liyam , Ercolano Emanuela , Kurian Kathreena M. , Hanemann C. Oliver , Belshaw Robert D. , Ammoun Sylwia
Published: April 2023

Supplementary Table 3
Table 3 and legend
Supplementary Figure 4 from ZIP9 Is a Druggable Determinant of Sex Differences in Melanoma
Authors: Aguirre-Portolés Cristina , Payne Riley , Trautz Aspen , Foskett J. Kevin , Natale Christopher A. , Seykora John T. , Ridky Todd W.
Published: April 2023

ZIP9 mediates testosterone effects in melanoma
Supplementary Figure 2 from ZIP9 Is a Druggable Determinant of Sex Differences in Melanoma
Authors: Aguirre-Portolés Cristina , Payne Riley , Trautz Aspen , Foskett J. Kevin , Natale Christopher A. , Seykora John T. , Ridky Todd W.
Published: April 2023

ZIP9 is active in human melanoma cells, is broadly expressed in human tumors.
Supplementary Figure 1 from ZIP9 Is a Druggable Determinant of Sex Differences in Melanoma
Authors: Aguirre-Portolés Cristina , Payne Riley , Trautz Aspen , Foskett J. Kevin , Natale Christopher A. , Seykora John T. , Ridky Todd W.
Published: April 2023

Biologic sex and testosterone promote proliferation of melanoma models lacking detectable AR.
Figure S4 from Human Endogenous Retrovirus Type K Promotes Proliferation and Confers Sensitivity to Antiretroviral Drugs in Merlin-Negative Schwannoma and Meningioma
Authors: Maze Emmanuel A. , Agit Bora , Reeves Shona , Hilton David A. , Parkinson David B. , Laraba Liyam , Ercolano Emanuela , Kurian Kathreena M. , Hanemann C. Oliver , Belshaw Robert D. , Ammoun Sylwia
Published: April 2023

Figure S4
Figure S4 and figure legend
Figure S1 from Human Endogenous Retrovirus Type K Promotes Proliferation and Confers Sensitivity to Antiretroviral Drugs in Merlin-Negative Schwannoma and Meningioma
Authors: Maze Emmanuel A. , Agit Bora , Reeves Shona , Hilton David A. , Parkinson David B. , Laraba Liyam , Ercolano Emanuela , Kurian Kathreena M. , Hanemann C. Oliver , Belshaw Robert D. , Ammoun Sylwia
Published: April 2023

Figure S1
Figure S1 and figure legend
Supplementary Table 4 from Diverse Oncogenic Fusions and Distinct Gene Expression Patterns Define the Genomic Landscape of Pediatric Papillary Thyroid Carcinoma
Authors: Stosic Ana , Fuligni Fabio , Anderson Nathaniel D. , Davidson Scott , de Borja Richard , Acker Meryl , Forte Vito , Campisi Paolo , Propst Evan J. , Wolter Nikolaus E. , Chami Rose , Mete Ozgur , Malkin David , Shlien Adam , Wasserman Jonathan D.
Published: April 2023

BRAF-RAS Score (BRS) Designation by Oncogenic Variant
Supplementary Figure 3 from Diverse Oncogenic Fusions and Distinct Gene Expression Patterns Define the Genomic Landscape of Pediatric Papillary Thyroid Carcinoma
Authors: Stosic Ana , Fuligni Fabio , Anderson Nathaniel D. , Davidson Scott , de Borja Richard , Acker Meryl , Forte Vito , Campisi Paolo , Propst Evan J. , Wolter Nikolaus E. , Chami Rose , Mete Ozgur , Malkin David , Shlien Adam , Wasserman Jonathan D.
Published: April 2023

Gene Set Variation Analysis
Supplementary Figure 2 from Diverse Oncogenic Fusions and Distinct Gene Expression Patterns Define the Genomic Landscape of Pediatric Papillary Thyroid Carcinoma
Authors: Stosic Ana , Fuligni Fabio , Anderson Nathaniel D. , Davidson Scott , de Borja Richard , Acker Meryl , Forte Vito , Campisi Paolo , Propst Evan J. , Wolter Nikolaus E. , Chami Rose , Mete Ozgur , Malkin David , Shlien Adam , Wasserman Jonathan D.
Published: April 2023

Expression individual genes comprising the TDS
Supplementary Data from Tumor Expression Quantitative Trait Methylation Screening Reveals Distinct CpG Panels for Deconvolving Cancer Immune Signatures
Authors: Yu Xiaoqing , Cen Ling , Chen Y. Ann , Markowitz Joseph , Shaw Timothy I. , Tsai Kenneth Y. , Conejo-Garcia Jose R. , Wang Xuefeng
Published: April 2023

Supplementary Data from Tumor Expression Quantitative Trait Methylation Screening Reveals Distinct CpG Panels for Deconvolving Cancer Immune Signatures
Table S7 from The Protein Kinase Activity of NME7 Activates Wnt/β-Catenin Signaling to Promote One-Carbon Metabolism in Hepatocellular Carcinoma
Authors: Ren Xinxin , Rong Zhuoxian , Liu Xiaoyu , Gao Jie , Xu Xu , Zi Yuyuan , Mu Yun , Guan Yidi , Cao Zhen , Zhang Yuefang , Zeng Zimei , Fan Qi , Wang Xitao , Pei Qian , Wang Xiang , Xin Haiguang , Li Zhi , Nie Yingjie , Qiu Zilong , Li Nan , Sun Lunquan , Deng Yuezhen
Published: April 2023

Supplemental Table 7 Correlation between the mRNA level of MTHFD2 and the clinical features of HCC.
Table S3 from The Protein Kinase Activity of NME7 Activates Wnt/β-Catenin Signaling to Promote One-Carbon Metabolism in Hepatocellular Carcinoma
Authors: Ren Xinxin , Rong Zhuoxian , Liu Xiaoyu , Gao Jie , Xu Xu , Zi Yuyuan , Mu Yun , Guan Yidi , Cao Zhen , Zhang Yuefang , Zeng Zimei , Fan Qi , Wang Xitao , Pei Qian , Wang Xiang , Xin Haiguang , Li Zhi , Nie Yingjie , Qiu Zilong , Li Nan , Sun Lunquan , Deng Yuezhen
Published: April 2023

Supplemental Table 3 The BIOCYC analysis for the kinase candidates.
Supplementary Data from The Protein Kinase Activity of NME7 Activates Wnt/β-Catenin Signaling to Promote One-Carbon Metabolism in Hepatocellular Carcinoma
Authors: Ren Xinxin , Rong Zhuoxian , Liu Xiaoyu , Gao Jie , Xu Xu , Zi Yuyuan , Mu Yun , Guan Yidi , Cao Zhen , Zhang Yuefang , Zeng Zimei , Fan Qi , Wang Xitao , Pei Qian , Wang Xiang , Xin Haiguang , Li Zhi , Nie Yingjie , Qiu Zilong , Li Nan , Sun Lunquan , Deng Yuezhen
Published: April 2023

Supplemental Materials and Methods and Supplemental Figures and Legends
Supplementary Data from Orthovoltage X-Rays Exhibit Increased Efficacy Compared with γ-Rays in Preclinical Irradiation
Authors: Bell Brett I. , Vercellino Justin , Brodin N. Patrik , Velten Christian , Nanduri Lalitha S.Y. , Nagesh Prashanth K.B. , Tanaka Kathryn E. , Fang Yanan , Wang Yanhua , Macedo Rodney , English Jeb , Schumacher Michelle M. , Duddempudi Phaneendra K. , Asp Patrik , Koba Wade , Shajahan Shahin , Liu Laibin , Tomé Wolfgang A. , Yang Weng-Lang , Kolesnick Richard , Guha Chandan
Published: April 2023

Supplementary Data from Orthovoltage X-Rays Exhibit Increased Efficacy Compared with γ-Rays in Preclinical Irradiation
Supplementary Data from Efficient Correction of Oncogenic KRAS and TP53 Mutations through CRISPR Base Editing
Authors: Sayed Shady , Sidorova Olga A. , Hennig Alexander , Augsburg Martina , Cortés Vesga Catherine P. , Abohawya Moustafa , Schmitt Lukas T. , Sürün Duran , Stange Daniel E. , Mircetic Jovan , Buchholz Frank
Published: April 2023

Supplementary Data from Efficient Correction of Oncogenic KRAS and TP53 Mutations through CRISPR Base Editing
Table ST1 from Regulatory Network of PD1 Signaling Is Associated with Prognosis in Glioblastoma Multiforme
Authors: Lopes-Ramos Camila M. , Belova Tatiana , Brunner Tess H. , Ben Guebila Marouen , Osorio Daniel , Quackenbush John , Kuijjer Marieke L.
Published: April 2023

We collected studies in glioblastoma that included survival thresholds through a PubMed search (search term: glioblastoma short-term prognostic signature). As can be seen from this table, a wide range of survival thresholds is used, with thresholds for short-term survival ranging between 7-24 months, and thresholds for long-term survival between 13-36 months after initial diagnosis. The threshold of 1.7yrs, which we chose for our comparison in the discovery datasets, corresponds to 20 months, which lies right in between these two ranges.
Supplementary Data 3 from N6-methyladenosine–Mediated Upregulation of WTAPP1 Promotes WTAP Translation and Wnt Signaling to Facilitate Pancreatic Cancer Progression
Authors: Deng Junge , Zhang Jialiang , Ye Ying , Liu Kaijing , Zeng Lingxing , Huang Jingyi , Pan Ling , Li Mei , Bai Ruihong , Zhuang Lisha , Huang Xudong , Wu Guandi , Wei Lusheng , Zheng Yanfen , Su Jiachun , Zhang Shaoping , Chen Rufu , Lin Dongxin , Zheng Jian
Published: April 2023

Clinical information for patients with IHC
Supplementary Data 2 from N6-methyladenosine–Mediated Upregulation of WTAPP1 Promotes WTAP Translation and Wnt Signaling to Facilitate Pancreatic Cancer Progression
Authors: Deng Junge , Zhang Jialiang , Ye Ying , Liu Kaijing , Zeng Lingxing , Huang Jingyi , Pan Ling , Li Mei , Bai Ruihong , Zhuang Lisha , Huang Xudong , Wu Guandi , Wei Lusheng , Zheng Yanfen , Su Jiachun , Zhang Shaoping , Chen Rufu , Lin Dongxin , Zheng Jian
Published: April 2023

WTAPP1 full length RNA pull down with mass spectrum
Supplementary Data 1 from N6-methyladenosine–Mediated Upregulation of WTAPP1 Promotes WTAP Translation and Wnt Signaling to Facilitate Pancreatic Cancer Progression
Authors: Deng Junge , Zhang Jialiang , Ye Ying , Liu Kaijing , Zeng Lingxing , Huang Jingyi , Pan Ling , Li Mei , Bai Ruihong , Zhuang Lisha , Huang Xudong , Wu Guandi , Wei Lusheng , Zheng Yanfen , Su Jiachun , Zhang Shaoping , Chen Rufu , Lin Dongxin , Zheng Jian
Published: April 2023

WTAPP1-m6A/WTAPP1 50bp RNA probe based Pull-down mass spectrum data
Table S1 from Targeting HER2 Exon 20 Insertion–Mutant Lung Adenocarcinoma with a Novel Tyrosine Kinase Inhibitor Mobocertinib
Authors: Han Han , Li Shuai , Chen Ting , Fitzgerald Michael , Liu Shengwu , Peng Chengwei , Tang Kwan Ho , Cao Shougen , Chouitar Johara , Wu Jiansheng , Peng David , Deng Jiehui , Gao Zhendong , Baker Theresa E. , Li Fei , Zhang Hua , Pan Yuanwang , Ding Hailin , Hu Hai , Pyon Val , Thakurdin Cassandra , Papadopoulos Eleni , Tang Sittinon , Gonzalvez Francois , Chen Haiquan , Rivera Victor M. , Brake Rachael , Vincent Sylvie , Wong Kwok-Kin
Published: April 2023

GSEA analysis of mobocertinib acquired resistant tumors versus response tumors
Genes involved in "G2M CHECKPOINT", "MITOTIC SPINDLE" and "MTORC1 SIGNALING" pathways of HALLMARK gene sets.
Figure S1 from Targeting HER2 Exon 20 Insertion–Mutant Lung Adenocarcinoma with a Novel Tyrosine Kinase Inhibitor Mobocertinib
Authors: Han Han , Li Shuai , Chen Ting , Fitzgerald Michael , Liu Shengwu , Peng Chengwei , Tang Kwan Ho , Cao Shougen , Chouitar Johara , Wu Jiansheng , Peng David , Deng Jiehui , Gao Zhendong , Baker Theresa E. , Li Fei , Zhang Hua , Pan Yuanwang , Ding Hailin , Hu Hai , Pyon Val , Thakurdin Cassandra , Papadopoulos Eleni , Tang Sittinon , Gonzalvez Francois , Chen Haiquan , Rivera Victor M. , Brake Rachael , Vincent Sylvie , Wong Kwok-Kin
Published: April 2023

Figure S1
Data from FOXP3–miR-146–NF-κB Axis and Therapy for Precancerous Lesions in Prostate
Authors: Liu Runhua , Yi Bin , Wei Shi , Yang Wei-Hsiung , Hart Karen M. , Chauhan Priyanka , Zhang Wei , Mao Xicheng , Liu Xiuping , Liu Chang-Gong , Wang Lizhong
Published: April 2023

Abstract
The tumor-suppressive activity of FOXP3 has been observed in tumor initiation, but the underlying mechanism still remains largely unknown. Here, we identified a FOXP3–microRNA-146 (miR-146)–NF-κB axis in vitro and in vivo in prostate cancer cells. We observed that FOXP3 dramatically induced the expression of miR-146a/b, which contributed to transcriptional inhibition of IRAK1 and TRAF6, in prostate cancer cell lines. Tissue-specific deletion of Foxp3 in mouse prostate caused a significant reduction of miR-146a and upregulation of NF-κB activation. In addition, prostatic intraepithelial neoplasia lesions were observed in miR-146a–mutant mice as well as in Foxp3-mutant mice. Notably, the NF-κB inhibitor bortezomib inhibited cell proliferation and induced apoptosis in prostate epithelial cells, attenuating prostatic intraepithelial neoplasia formation in Foxp3-mutant mice. Our data suggest that the FOXP3–miR-146–NF-κB axis has a functional role during tumor initiation in prostate cancer. Targeting the miR-146–NF-κB axis may provide a new therapeutic approach for prostate cancers with FOXP3 defects. Cancer Res; 75(8); 1714–24. ©2015 AACR.
Data from Constitutive Activation of Myosin-Dependent Contractility Sensitizes Glioma Tumor-Initiating Cells to Mechanical Inputs and Reduces Tissue Invasion
Authors: Wong Sophie Y. , Ulrich Theresa A. , Deleyrolle Loic P. , MacKay Joanna L. , Lin Jung-Ming G. , Martuscello Regina T. , Jundi Musa A. , Reynolds Brent A. , Kumar Sanjay
Published: April 2023

Abstract
Tumor-initiating cells (TIC) perpetuate tumor growth, enable therapeutic resistance, and drive initiation of successive tumors. Virtually nothing is known about the role of mechanotransductive signaling in controlling TIC tumorigenesis, despite the recognized importance of altered mechanics in tissue dysplasia and the common observation that extracellular matrix (ECM) stiffness strongly regulates cell behavior. To address this open question, we cultured primary human glioblastoma (GBM) TICs on laminin-functionalized ECMs spanning a range of stiffnesses. Surprisingly, we found that these cells were largely insensitive to ECM stiffness cues, evading the inhibition of spreading, migration, and proliferation typically imposed by compliant ECMs. We hypothesized that this insensitivity may result from insufficient generation of myosin-dependent contractile force. Indeed, we found that both pharmacologic and genetic activation of cell contractility through RhoA GTPase, Rho-associated kinase, or myosin light chain kinase restored stiffness-dependent spreading and motility, with TICs adopting the expected rounded and nonmotile phenotype on soft ECMs. Moreover, constitutive activation of RhoA restricted three-dimensional invasion in both spheroid implantation and Transwell paradigms. Orthotopic xenotransplantation studies revealed that control TICs formed tumors with classical GBM histopathology including diffuse infiltration and secondary foci, whereas TICs expressing a constitutively active mutant of RhoA produced circumscribed masses and yielded a 30% enhancement in mean survival time. This is the first direct evidence that manipulation of mechanotransductive signaling can alter the tumor-initiating capacity of GBM TICs, supporting further exploration of these signals as potential therapeutic targets and predictors of tumor-initiating capacity within heterogeneous tumor cell populations. Cancer Res; 75(6); 1113–22. ©2015 AACR.
Data from CLK2 Is an Oncogenic Kinase and Splicing Regulator in Breast Cancer
Authors: Yoshida Taku , Kim Jee Hyun , Carver Kristopher , Su Ying , Weremowicz Stanislawa , Mulvey Laura , Yamamoto Shoji , Brennan Cameron , Mei Shenglin , Long Henry , Yao Jun , Polyak Kornelia
Published: April 2023

Abstract
Genetically activated kinases have been attractive therapeutic targets in cancer due to the relative ease of developing tumor-specific treatment strategies for them. To discover novel putative oncogenic kinases, we identified 26 genes commonly amplified and overexpressed in breast cancer and subjected them to a lentiviral shRNA cell viability screen in a panel of breast cancer cell lines. Here, we report that CLK2, a kinase that phosphorylates SR proteins involved in splicing, acts as an oncogene in breast cancer. Deregulated alternative splicing patterns are commonly observed in human cancers but the underlying mechanisms and functional relevance are still largely unknown. CLK2 is amplified and overexpressed in a significant fraction of breast tumors. Downregulation of CLK2 inhibits breast cancer growth in cell culture and in xenograft models and it enhances cell migration and invasion. Loss of CLK2 in luminal breast cancer cells leads to the upregulation of epithelial-to-mesenchymal transition (EMT)-related genes and a switch to mesenchymal splice variants of several genes, including ENAH (MENA). These results imply that therapeutic targeting of CLK2 may be used to modulate EMT splicing patterns and to inhibit breast tumor growth. Cancer Res; 75(7); 1516–26. ©2015 AACR.
Data from Candidate Antimetastasis Drugs Suppress the Metastatic Capacity of Breast Cancer Cells by Reducing Membrane Fluidity
Authors: Zhao Weina , Prijic Sara , Urban Bettina C. , Tisza Michael J. , Zuo Yan , Li Lin , Tan Zhi , Chen Xiaoling , Mani Sendurai A. , Chang Jeffrey T.
Published: April 2023

Abstract
Despite the high mortality from metastatic cancer, therapeutic targets to prevent metastasis are limited. Efforts to identify genetic aberrations that predispose tumors to metastasis have been mostly unsuccessful. To understand the nature of candidate targets for metastatic disease, we performed an in silico screen to identify drugs that can inhibit a gene expression signature associated with epithelial–mesenchymal transition (EMT). Compounds discovered through this method, including those previously identified, appeared to restrict metastatic capacity through a common mechanism, the ability to modulate the fluidity of cell membranes. Treatment of breast cancer cell lines with the putative antimetastasis agents reduced membrane fluidity, resulting in decreased cell motility, stem cell–like properties, and EMT in vitro, and the drugs also inhibited spontaneous metastasis in vivo. When fluidity was unchanged, the antimetastasis compounds could no longer restrict metastasis, indicating a causal association between fluidity and metastasis. We further demonstrate that fluidity can be regulated by cellular cholesterol flux, as the cholesterol efflux channel ABCA1 potentiated metastatic behaviors in vitro and in vivo. The requirement for fluidity was further supported by the finding in breast cancer patients that ABCA1 was overexpressed in 41% of metastatic tumors, reducing time to metastasis by 9 years. Collectively, our findings reveal increased membrane fluidity as a necessary cellular feature of metastatic potential that can be controlled by many currently available drugs, offering a viable therapeutic opportunity to prevent cancer metastasis. Cancer Res; 76(7); 2037–49. ©2016 AACR.
Data from Hyperthermia Synergizes with Chemotherapy by Inhibiting PARP1-Dependent DNA Replication Arrest
Authors: Schaaf Lea , Schwab Matthias , Ulmer Christoph , Heine Simon , Mürdter Thomas E. , Schmid Jens O. , Sauer Georg , Aulitzky Walter E. , van der Kuip Heiko
Published: April 2023

Abstract
Although hyperthermia offers clinical appeal to sensitize cells to chemotherapy, this approach has been limited in terms of long-term outcome as well as economic and technical burden. Thus, a more detailed knowledge about how hyperthermia exerts its effects on chemotherapy may illuminate ways to improve the approach. Here, we asked whether hyperthermia alters the response to chemotherapy-induced DNA damage and whether this mechanism is involved in its sensitizing effect in BRCA-competent models of ovarian and colon cancer. Notably, we found that hyperthermia delayed the repair of DNA damage caused by cisplatin or doxorubicin, acting upstream of different repair pathways to block histone polyADP-ribosylation (PARylation), a known effect of chemotherapy. Furthermore, hyperthermia blocked this histone modification as efficiently as pharmacologic inhibitors of PARP (PARPi), producing comparable delay in DNA repair, induction of double-strand breaks (DSB), and cell cytotoxicity after chemotherapy. Mechanistic investigations indicated that inhibiting PARylation by either hyperthermia or PARPi induced lethal DSB upon chemotherapy treatment not only by reducing DNA repair but also by preventing replication fork slowing. Overall, our work reveals how PARP blockade, either by hyperthermia or small-molecule inhibition, can increase chemotherapy-induced damage in BRCA-competent cells. Cancer Res; 76(10); 2868–75. ©2016 AACR.
Data from Bereavement Is Associated with an Increased Risk of HPV Infection and Cervical Cancer: An Epidemiological Study in Sweden
Authors: Lu Donghao , Sundström Karin , Sparén Pär , Fall Katja , Sjölander Arvid , Dillner Joakim , Helm Nathalie Ylitalo , Adami Hans-Olov , Valdimarsdóttir Unnur , Fang Fang
Published: April 2023

Abstract
Grief over the loss of a family member may cause physical and mental illness, but an association between bereavement and cancer risk has not been established. Based on the Swedish National Cervical Screening Register (1969–2011) including 14,011,269 smears from 2,466,107 women, we conducted two nested case–control studies to examine the associations of bereavement (i.e., loss of a family member due to death) with abnormal cytology (390,310 first abnormal and 1,951,319 normal smears) and in situ/invasive cervical cancer (75,128 case and 375,640 control women), both individually matched on year of birth and screening adherence. Among 1,696 of the control women, we further investigated bereavement in association with human papillomavirus (HPV) infection, both HPV16 and other HPV types. Bereavement was consistently associated with a 4% to 9% increased risk for first abnormal cytology, in situ and invasive cervical cancer (all P < 0.02). The associations became stronger when multiple losses, loss of child, sibling or spouse, and loss due to unnatural cause were analyzed separately (P for trend or difference < 0.0001), and for women with high screening adherence (P for difference < 0.05). Among 1,696 women who had not developed cervical cancer, we further investigated the link between bereavement and HPV infection. Bereavement was associated with a 62% increased risk of HPV16 infection, high viral load, and recurrent infection, and was also more strongly associated with HPV infections designated as high-risk compared with low-risk determinants of cervical carcinogenesis. Collectively, our findings demonstrate that bereavement is associated with an increased risk of developing cervical cancer. Further, they suggest that this association may be attributed to stress-induced oncogenic HPV infections. Cancer Res; 76(3); 643–51. ©2015 AACR.
Data from Downregulation of the TGFβ Pseudoreceptor BAMBI in Non–Small Cell Lung Cancer Enhances TGFβ Signaling and Invasion
Authors: Marwitz Sebastian , Depner Sofia , Dvornikov Dmytro , Merkle Ruth , Szczygieł Magdalena , Müller-Decker Karin , Lucarelli Philippe , Wäsch Marvin , Mairbäurl Heimo , Rabe Klaus F. , Kugler Christian , Vollmer Ekkehard , Reck Martin , Scheufele Swetlana , Kröger Maren , Ammerpohl Ole , Siebert Reiner , Goldmann Torsten , Klingmüller Ursula
Published: April 2023

Abstract
Non–small cell lung cancer (NSCLC) is characterized by early metastasis and has the highest mortality rate among all solid tumors, with the majority of patients diagnosed at an advanced stage where curative therapeutic options are lacking. In this study, we identify a targetable mechanism involving TGFβ elevation that orchestrates tumor progression in this disease. Substantial activation of this pathway was detected in human lung cancer tissues with concomitant downregulation of BAMBI, a negative regulator of the TGFβ signaling pathway. Alterations of epithelial-to-mesenchymal transition (EMT) marker expression were observed in lung cancer samples compared with tumor-free tissues. Distinct alterations in the DNA methylation of the gene regions encoding TGFβ pathway components were detected in NSCLC samples compared with tumor-free lung tissues. In particular, epigenetic silencing of BAMBI was identified as a hallmark of NSCLC. Reconstitution of BAMBI expression in NSCLC cells resulted in a marked reduction of TGFβ-induced EMT, migration, and invasion in vitro, along with reduced tumor burden and tumor growth in vivo. In conclusion, our results demonstrate how BAMBI downregulation drives the invasiveness of NSCLC, highlighting TGFβ signaling as a candidate therapeutic target in this setting. Cancer Res; 76(13); 3785–801. ©2016 AACR.