bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimating Water Content in Water-oil Mixtures and Porous Media they Saturate: Joint Interpretation of NMR Relaxometry and Dielectric Spectroscopy
Authors: Shumskayte Mariya , Mezin Andrey , Chernova Elena , Burukhina Aleksandra , Golikov Nikita , Melkozerova Svetlana
Published: December 2021

The article is devoted to the topical problem of estimating water content in water-oil mixtures and porous media they saturate, according to low-field NMR relaxometry and dielectric spectroscopy. The aim of the research is to theoretically substantiate and experimentally validate the capability of joint interpretation of data from these methods to acquire information on the filtration-volumetric properties of drill cuttings, relaxation characteristics of oil-containing fluids, water/oil ratio in water-oil mixtures and saturated with them drill cuttings in order to control the composition of liquids produced from boreholes. The studies were carried out on samples of cuttings and oils taken from fields in the northern and Arctic regions of the West Siberian oil-and-gas province. Based on the experimental data obtained, we evaluated the water content in the water-oil mixtures, determined the main NMR parameters of the mixtures in terms of properties of the constituent oils, and specified the parameters and shapes of NMR and complex dielectric permittivity spectra. The NMR method was found to be effective in examining high-viscosity and medium-viscosity oils, while the dielectric spectroscopy method – in the study of light oils; their integration allows obtaining reliable data for all the samples under study. We also showed how the shapes of NMR and complex dielectric permittivity spectra depend on the rheological properties of oil belonging to the mixture.
Circadian Gene cry Controls Glioblastoma Tumorigenesis through Modulation of myc Expression
Authors: Jarabo Patricia , de Pablo Carmen , González-Blanco Amanda , Casas-Tintó Sergio
Published: December 2021

Glioblastoma (GB) is the most frequent malignant brain tumor among adults and currently there is no effective treatment. It is a very aggressive tumor that grows fast and spreads through the brain causing the death of patients in 15 months. GB cells mutate frequently and generate a heterogeneous population of tumoral cells genetically distinct. Thus, the contribution of genes and signaling pathways relevant for GB progression is of great relevance. We use a Drosophila model of GB that reproduces the features of human GB, and describe the upregulation of the circadian gene cry in GB patients and in a Drosophila GB model. We study the contribution of cry to the expansion of GB cells, to the neurodegeneration caused by GB, and to premature death and determine that cry is required for GB progression. Moreover, we analyze the mechanisms that regulate cry expression by the PI3K pathway. Finally, we conclude that cry is necessary and sufficient to regulate myc expression in GB. These results contribute to the understanding of the signals that impulse GB malignancy and lethality and open novel opportunities for the treatment of GB patients.
Terapie genowe i genetyczne w chorobach nerwowo- mięśniowych wieku dziecięcego
Authors:   Oddział Kliniczny Neurologii Dzieci i Młodzieży, Szpital Kliniczny im. H. Święcickiego UM w Poznaniu, Katedra i Klinika Neurologii Wieku Rozwojowego Uniwersytet Medyczny im. K. Marcinkowskiego w Poznaniu, Jączak-Goździak Małgorzatak , Steinborn Barbara ,   Katedra i Klinika Neurologii Wieku Rozwojowego UM im. K. Marcinkowskiego w Poznaniu, Oddział Kliniczny Neurologii Dzieci i Młodzieży, Szpital Kliniczny im. H. Święcickiego UM w Poznaniu
Published: December 2021

Osiągnięcia genetyki, biologii molekularnej i inżynierii genetycznej zrewolucjonizowały współczesną medycynę: umożliwiły poznanie i zrozumienie genetycznego podłoża wielu chorób i utorowały drogę do poszukiwania leczenia poprzez naprawę wadliwych genów. W ostatnich latach największe sukcesy w zakresie terapii genowej i genetycznej zostały osiągnięte w chorobach nerwowo-mięśniowych wieku dziecięcego: w rdzeniowym zaniku mięśni i dystrofii mięśniowej Duchenne'a. To nowoczesne leczenie wykorzystuje różnorodne techniki takie jak: transport genów za pomocą wektorów wirusowych czy modyfikację ekspresji genów z użyciem antysensownych oligonukleotydów i "małych cząsteczek" oraz jeszcze badaną, ale bardzo obiecującą, edycję genomu z zastosowaniem systemu CRISPR-Cas9. W pracy przedstawiono przegląd: przełomowych terapii w leczeniu SMA i DMD oraz leczenia genetycznego, badanego w innych chorobach nerwowo- mięśniowych wieku dziecięcego
Polymorphisms variants of the MTHFR C677T and PAI-1 5G/4G genes and their combinations in the group of children with arterial ischemic stroke
Authors:   Neurological Department, City Clinical Children's Hospital Nº1 in Kyiv, Ukraine, Smulska Nataliya , Rossokha Zoia ,   Institution Reference-centre for Molecular Diagnostic of Public Health Ministry of Ukraine, Kyiv, Ukraine, Fishchuk Liliya ,   Institution Reference-centre for Molecular Diagnostic of Public Health Ministry of Ukraine, Kyiv, Ukraine, Gorovenko Nataliya ,   The National Medical Academy of Postgraduate Education State named after P.L. Shupyk, Kyiv, Ukraine, Nechai Alla ,   Neurological Department, City Clinical Children's Hospital Nº1 in Kyiv, Ukraine, Nicolaenko Iryna ,   Neurological Department, City Clinical Children's Hospital Nº1 in Kyiv, Ukraine, Rysliaieva Viktotiia ,   Neurological Department, City Clinical Children's Hospital Nº1 in Kyiv, Ukraine, Krasilenko Iryna ,   Neurological Department, City Clinical Children's Hospital Nº1 in Kyiv, Ukraine
Published: December 2021

Introduction. Arterial ischemic stroke (AIS) in childhood is a disorder associated with different predisposing factors, thrombophilia is one of them. We present the study of the MTHFR C677T and PAI-1 5G/4G gene polymorphisms as a possible risk factor for the development of AIS in the group of Ukrainian children. Materials and methods. 77 children from 29 days to 15 years of age were involved in this study: study group - 44 children with AIS, and 33 in a control group. Children enrolled to both groups were at similar age. Results. In the study group there was an increase in the frequency of genotypes 677CT (OR = 2.69) and 677TT, CT + TT genotypes (OR = 3.67) of the MTHFR gene, increased frequency of the 677T allele (OR = 2.57). In the study group detection of 5G/5G + 5G/4G genotypes was higher (OR = 2.82) with statistically predominance of the 5G allele (OR = 2.26) of the gene PAI -1. Higher frequency of the combination of genotypes genes 677CT + 5G/5G was found in the main group. The model of interpreting interactions was built, it allows to predict indirectly the potential intergenic interactions. Conclusions. We conclude that risk of AIS is higher in children with polymorphic variants 677CT and 677TT for the MTHFR gene; the association of polymorphic variants 5G/4G and 5G/5G for the PAI-1 gene with a decrease in the risk of developing AIS; direct interaction with the MTHFR was found for PAI-1, but of weak strength
Asymetria mózgu jako objaw radiologiczny pomocny w dalszej diagnostyce neurologicznej -przegląd kliniczno-radiologiczny wybranych jednostek neurologicznych związanych z asymetrią budowy mózgu
Authors:   Oddział Kliniczny Neurologii Dzieci i Młodzieży, Szpital Kliniczny im. H. Święcickiego UM w Poznani, Jączak-Goździak Małgorzata , Stajgis Marek ,   atedra Radiologii Ogólnej i Neuroradiologii UM im. K. Marcinkowskiego w Poznaniu,  Szpital Kliniczny im. H. Święcickiego    UM w Poznaniu, Steinborn Barbara ,   atedra i Klinika Neurologii Wieku Rozwojowego UM im. K. Marcinkowskiego w Poznaniu, Oddział Kliniczny Neurologii   Dzieci i Młodzieży, Szpital Kliniczny im. H. Święcickiego UM w Poznaniu
Published: December 2021

Asymetria półkul mózgu jest zjawiskiem fizjologicznym, a dominacja jednej ze stron, zwłaszcza czynnościowa, jest szczególnie wyraźna w sferze funkcji poznawczych. W odróżnieniu do fizjologicznej, w patologicznej nierówności półkul mózgowych występują znaczne różnice w budowie anatomicznej i histologicznej oraz pojawiają się objawy takie jak niedowłady, napady padaczkowe, zaburzenia funkcji poznawczych czy zaburzenia ruchowe. W artykule przedstawiono wybrane zespoły powiązane z asymetrią półkul mózgu, dokonano klasyfikacji ze względu na etiologię oraz przebieg (na postępujące i niepostępujące), przedstawiono ich kliniczną oraz radiologiczną charakterystykę.
Hypertonia Assessment Tool – użyteczne narzędzie do różnicowania wygórowanego napięcia mięśniowego u pacjentów pediatrycznych
Authors:   Oddział Fizjoterapii, Wydział Medyczny, Warszawski Uniwersytet Medyczny, Ferenstein Maria , Piotrowski Tomasz A. ,   Oddział Fizjoterapii, Wydział Medyczny, Warszawski Uniwersytet Medyczny, Stańczyk Małgorzata J. ,   Oddział Fizjoterapii, Wydział Medyczny, Warszawski Uniwersytet Medyczny, Tekień Edyta ,   Oddział Fizjoterapii, Wydział Medyczny, Warszawski Uniwersytet Medyczny, Gąsior Jakub S. ,   Klinika Kardiologii Wieku Dziecięcego i Pediatrii Ogólnej, Warszawski Uniwersytet Medyczny
Published: December 2021

Hypertonia Assessment Tool (HAT) stanowi użyteczne narzędzie do oceny i klasyfikacji wygórowanego napięcia mięśniowego, i może być stosowane podczas badania pacjentów pediatrycznych z zaburzeniami neurologicznymi. Prawidłowa ocena rodzaju hipertonii (dystonia, spastyczność, sztywność) jest kluczowa w doborze odpowiedniego leczenia i ustalania programu rehabilitacji. Celem pracy było omówienie praktycznego wykorzystania narzędzia HAT u pacjentów pediatrycznych oraz dokonanie przeglądu piśmiennictwa badań wykorzystujących to narzędzie do oceny napięcia mięśniowego. Przegląd piśmiennictwa przeprowadzono zgodnie z rekomendacjami PRISMA przeszukując medyczną bazę danych Pubmed. Uwzględniono jedynie publikacje anglojęzyczne. Zidentyfikowano 5 publikacji spełniających powyższe kryteria, oraz jeden komentarz. Autorzy włączonych badań sugerują, że HAT jest przydatne do wykrywania sztywności i spastyczności, natomiast przy dystonii zaleca się dokładniejszą analizę i modyfikację użycia tego narzędzia. HAT charakteryzuje się dobrą wiarygodnością i trafnością w identyfikacji spastyczności i braku sztywności, a także umiarkowaną dokładności rozpoznawczą w przypadku dystonii. Przedstawiony artykuł przedstawia tłumaczenie własne kwestionariusza wykorzystywanego podczas badania hipertonii oraz opis konstrukcji i zalet narzędzia. Zaleca się wykorzystywanie narzędzia HAT do oceny rodzaju podwyższonego napięcia mięśniowego u pacjentów pediatrycznych z zaburzeniami neurologicznymi.
Guillain-Barre syndrome as a paraneoplastic syndrome in a 17-year- old boy
Authors:   Department of Neurology and Pediatrics, Medical University of Warsaw, Poland, Dryżałowski Paweł , Sołowiej Elżbieta ,   Department of Neurology and Pediatrics, Medical University of Warsaw, Poland, Okruciński Damian ,   Medical University of Warsaw, Poland, Jóźwiak Sergiusz ,   Department of Neurology and Pediatrics, Medical University of Warsaw, Poland
Published: December 2021

Guillain-Barre syndrome (GBS) is an acute inflammatory polyneuropathy, characterised by progressive, symmetrical muscle weakness and sensory disorder due to autoimmunologic myelin nerve sheats and/or peripheral nerves axonal damage. The course of the disease in children is usually milder than in adults. The most common variant of GBS is acute inflammatory demyelinating polyneuropathy (AIDP). GBS is a rare disorder with morbidity rate of 0,5-1,5/100 000/ year, more often seen in males. The course of disease in children is usually milder than in adults. Early diagnosis and proper treatment enables complete recovery in 80% of cases, while approximately 10% of patients suffer from symptoms recurrence, mainly after infection. Almost 2/3 of GBS cases are preceded by upper respiratory or gastrointestinal infection. The emergence of antibodies in various mechanisms, which cross react with nerve sheats or axon antigens (through a phenomenon known as molecular mimicry), leads to development of the syndrome. Known triggers inducing GBS include viral and bacterial infections, injuries, surgery, bone marrow transplantation and rarely childhood vaccinations. In still rarer cases, GBS may develop in the course of paraneoplastic syndrome (PNS), and be the first symptom of an underlying neoplastic process.
Korelasi Antar Karakter Pertumbuhan Dan Hasil Sepuluh Genotipe Talas Jepang pada Tiga Agroekologi Berbeda
Authors: Maretta Delvi , Sobir Sobir , Helianti Is , Purwono Purwono , Santosa Edi
Published: December 2021

Talas eddoe (Colocasia esculenta var antiquorum) atau talas Jepang merupakan tanaman yang prospektif dikembangkan di Indonesia. Informasi karakter tanaman talas pada berbagai agroekologi sangat penting sebagai dasar seleksi dan pemilihan lokasi dalam program pemuliaan tanaman. Penelitian bertujuan untuk mengetahui korelasi antar karakter pertumbuhan dan hasil tanaman talas Jepang yang ditanam pada tiga agroekologi yang berbeda. Percobaan dilaksanakan pada tahun 2018 sampai 2019 di Tangerang Selatan, Bogor dan Subang menggunakan sepuluh genotipe talas eddoe. Hasil penelitan menunjukkan bahwa panjang daun, panjang petiol dan panjang pelepah antargenotipe memiliki perbedaan yang nyata. Lokasi penanaman juga berpengaruh nyata terhadap semua karakter pertumbuhan dan hasil. Genotipe dan lokasi berinteraksi nyata terhadap panjang daun, petiol total, rentang tanaman, tinggi tanaman, panjang dan berat cormus. Karakter pertumbuhan dan hasil selalu berkorelasi erat di tiga lokasi, sedangkan korelasi karakter pertumbuhan dengan karakter hasil talas berbeda di tiap agroekologi (r=0,05). Perbedaan lingkungan tumbuh sangat berpengaruh terhadap nilai dan keeratan korelasi karakter pertumbuhan dengan hasil, sehingga upaya peningkatan hasil perlu memperhatikan agroekologi yang sesuai bagi pertumbuhan tanaman talas.
Novel Ultrasonographic Thickness and Strength Assessments of the Flexor Digitorum: A Reliability Analysis
Authors: Ioannou Christos I , Hodde-Chriske Franziska L , Avraamides Marios N , Altenmüller Eckart
Published: December 2021

OBJECTIVES: Clinical conditions such as focal dystonia often require the assessment of atrophy and weakness of the finger muscles. However, due to a lack of well-established protocols, the current investigation focused on assessing the reliability of thickness and strength assessments
of the flexor digitorum (FD) muscle, including both the superficialis and profundus components. As a secondary assessment, the reliability of the strength measurement of the extensor digitorum muscle was examined as well. METHODS: Different thickness measurements of the FD were taken via ultrasonography
and averaged to estimate the mean thickness of the FD. Likewise, individual finger strength measurements taken by a custom-made finger pressure device were averaged to compute the mean strength of the flexor and extensor digitorum muscles. Test-retest reliability of the above measurements
performed at two different time points (about 6 months apart) were examined on the right and left hands of 10 participants. RESULTS: Findings indicated excellent test-retest reliability (ICC > 0.92) for the mean thickness assessment of the FD and mean strength of the flexor and extensor
digitorum for both dominant and non-dominant hands. The standard error of measurement was ≤4.3% for all three mean assessments, indicating high sensitivity. Likewise, the smallest detectable change was also sufficiently small for the mean thickness and mean strength of the flexor digitorum
(≤5.1%) and moderately small (≤12%) for the strength of the extensor digitorum. CONCLUSIONS: Results indicated an excellent relative and absolute reliability, for both hands, for the mean thickness and strength assessments of the flexor digitorum muscle and for the mean strength of the
extensor digitorum (measured for both hands). These measurements can be used for future investigations and can contribute to the establishment of more precise methods for assessing the muscles in the forearms which serve the hand.
Relationship Between Plasma Cell-Free DNA Changes and Lysyl Oxidase During the Treatment and Prognosis of Canine Transmissible Venereal Tumor
Authors: MohammadZahri Mona , Cheraghi Hadi , Shirani Dariush , Hatamkhani Ali
Published: December 2021

BackgroundTransmissible Venereal Tumor (TVT) is a wide tumor of canine, there are no effective markers to monitor the therapeutic response in real-time. Circulating biomarkers may be valuable for the early diagnosis and prognosis of cancers, so in this study, we aimed to investigate the significance of the cell-free DNA (cfDNA) and cfDNA integrity index to monitor the response of TVT to vincristine and compare them with lysyl oxidase activity. Plasma and sera were collected before drug administration within four weeks from fifteen male dogs. The analytical method was mainly based on quantitative polymerase chain reaction for short and long cfDNA, and lysyl oxidase activity was measured in serum.ResultsThe results of cfDNA integrity index showed significant (p<0.05) difference in a baseline to 2nd and 3rd week (with cut-off value 1.118 and 93.33% specificity). We found that the cfDNA integrity index was increased during weeks due to the reduction of shorts cfDNA in the 1st week after treatment. lysyl Oxidase activity was increased during the 4th week (p<0.001) but there were no significant differences in the other weeks compared to baseline. ROC analysis of lysyl Oxidase revealed high sensitivity (100%) and specificity (93%) 2nd, 3rd weeks on comparison between Baseline. Multivariate analysis between cfDNA integrity index and lysyl Oxidase showed significant correlation (p<0.05) only baseline results. ConclusionsTaken together, we propose short cfDNA, cfDNA integrity index, and lysyl Oxidase activity as a diagnostic biomarker and a putative prognostic candidate in TVT patients. These biomarkers could be used simultaneously for quickly diagnose TVT in combination with cytology.
Factors Associated With Feeding And Caregiving Practices For Children Under Five In Lowland Nepal: A Community-Based Cross-Sectional Survey
Authors: Dulal Sophiya , Prost Audrey , Karki Surendra , Merom Dafna , Shrestha Bhim Prasad , Bhandari Bishnu , Manandhar Dharma Sharan , Osrin David , Costello Anthony , Saville Naomi M.
Published: December 2021

Background: While nurturing care, including adequate nutrition and responsive caregiving and early learning, is critical to early childhood development, national surveys in Nepal highlight inequalities in feeding and parental caregiving practices. This study aimed to document and analyse infant and young child feeding (IYCF) and cognitive and socio-emotional caregiving practices among caregivers of children under age five in Dhanusha district, Nepal. Methods: We used a subset of data from the MIRA Dhanusha cluster randomised controlled trial, including mother-child dyads (N=1360) when children were aged 0-12 months and a follow-up survey of the same mother-child dyads (N=1352) when children were 7-59 months. We used World Health Organization IYCF indicators and questions from the Multiple Indicator Cluster Survey-4 tool to obtain information on IYCF and cognitive and socio-emotional caregiving practices, respectively. We collected data on breastfeeding practices in a postpartum 6-week questionnaire and on complementary feeding and caregiving practices in the follow-up survey. Using multivariable logistic regression models, potential explanatory household, parental and child-level variables were tested to determine their independent associations with IYCF and caregiving indicators. Results: The prevalence of feeding indicators varied. Ever breastfed (99%), exclusive breastfeeding in past 24-hours at 0-12 months (89%), and vegetable or fruit consumption (84%) were common, and minimum dietary diversity reached 63%. Problem areas were early initiation of breastfeeding (16%), feeding of colostrum (67%), no pre-lacteal feeding (53%), timely introduction of complementary feeding (56%), and animal-source food consumption (29%). Amongst caregiving indicators, access to books (7%), early stimulation and responsive caregiving (11%), and access to early childhood education (27%) were of particular concern, while 64% had access to toys and 71% received adequate care. Only 38% of children were developmentally on track. Younger children from poor households with young mothers who had not received antenatal visits and delivered at home were at risk of poor IYCF and caregiving practices. Conclusions: Poor IYCF and cognitive and socio-emotional caregiving practices are widely prevalent in lowland Nepal, which highlights the importance of improved integrated nutrition and caregiving interventions in resource-constrained settings.
tRNA anticodon cleavage by target-activated CRISPR-Cas13a effector
Authors: Semenova Ekaterina , Jain Ishita , Kolesnik Matvey , Minakhin Leonid , Morozova Natalia , Shiriaeva Anna , Kirillov Alexandr , Medvedeva Sofya , Kuznedelov Konstantin , Borukhov Sergei , Makarova Kira , Koonin Eugene V. , Severinov Konstantin
Published: December 2021

Type VI CRISPR-Cas systems are the only CRISPR variety that cleaves exclusively RNA1,2. In addition to the CRISPR RNA (crRNA)-guided, sequence-specific binding and cleavage of target RNAs, such as phage transcripts, the type VI effector, Cas13, causes collateral RNA cleavage, which induces bacterial cell dormancy, thus protecting the host population from phage spread3,4. We show here that the principal form of collateral RNA degradation elicited by Cas13a protein from Leptotrichia shahii upon target RNA recognition is the cleavage of anticodons of multiple tRNA species, primarily those with anticodons containing uridines. This tRNA cleavage is necessary and sufficient for bacterial dormancy induction by Cas13a. In addition, Cas13a activates the RNases of bacterial toxin-antitoxin modules, thus indirectly causing mRNA and rRNA cleavage, which could provide a back-up defense mechanism. The identified mode of action of Cas13a resembles that of bacterial anticodon nucleases involved in antiphage defense5, which is compatible with the hypothesis that type VI effectors evolved from an abortive infection module6,7 encompassing an anticodon nuclease.
Flooding affects food web structure and basal sources supporting fish guilds in a subtropical wetland and shallow lake
Authors: Bastos Rodrigo Ferreira , Garcia Alexandre Miranda , Winemiller Kirk O. , Fontoura Nelson Ferreira
Published: December 2021

Aquatic ecosystems exchange nutrients and organic matter with surrounding terrestrial ecosystems, and floods import allochthonous material from riparian areas into fluvial systems. We surveyed food web components of a wetland and shallow lake in a subtropical coastal region of Brazil to examine how community trophic structure and the entrance of allochthonous material into the food web were affected by floods. Stable isotope analysis was performed for samples of terrestrial and aquatic basal production sources and aquatic animals to trace the origin of organic matter assimilated by aquatic animals and estimate vertical trophic positions and food chain length. Lake and wetland trophic structures were compared for cool/wet and warm/dry seasons. Food web structure was hypothesized to differ based on hydrology, with the more stable lake having greater food web complexity, and seasonal flooding resulting in greater allochthonous inputs to the aquatic food web. We compared spatial and temporal variation in assemblage trophic structure using an adapted isotopic ellipse approach that plots assemblage elements according to δ13C on the x-axis and estimated TP on the y-axis. Lake trophic structure was more complex with longer food chains compared to that of the wetland. A greater contribution from terrestrial resources to animal biomass was observed in the wetland during the cool/wet period, and food chains in both habitats tended to be longer during the cool/wet period. Findings supported the hypothesis of greater assimilation of allochthonous sources during floods and greater trophic complexity in the more hydrologically stable system.
Chk1 Inhibitor Induced DNA Damage Increases BFL1 and Decreases BIM but Does Not Protect Human Cancer Cell Lines From Chk1 Inhibitor-induced Apoptosis
Authors: Massey Andrew J.
Published: December 2021

V158411 is a potent, selective Chk1 inhibitor currently in pre-clinical development. We utilised RNAseq to evaluate the gene responses to V158411 treatment. BCL2A1 was highly upregulated in U2OS cells in response to V158411 treatment with BCL2A1 mRNA increased >400-fold in U2OS but not HT29 cells. Inhibitors of Chk1, Wee1 and topoisomerases but not other DNA damaging agents or inhibitors of ATR, ATM or DNA-PKcs increased BFL1 and decreased BIM protein. Increased BFL1 appeared limited to a subset of approximately 35% of U2OS cells. Out of 24 cell lines studied, U2OS cells were unique in being the only cell line with low basal BFL1 levels to be increased in response to DNA damage. Induction of BFL1 in U2OS cells appeared dependent on PI3K/AKT/mTOR/MEK pathway signalling but independent of NF-κB transcription factors. Inhibitors of MEK, mTOR and PI3K effectively blocked the increase in BFL1 following V15841 treatment. Increased BFL1 expression did not block apoptosis in U2OS cells in response to V158411 treatment and cells with high basal expression of BFL1 readily underwent caspase-dependent apoptosis following Chk1 inhibitor therapy. BFL1 induction in response to Chk1 inhibition appeared to be a rare event that was dependent on MEK/PI3K/AKT/mTOR signalling.
A Lightweight Keypoint Matching Framework for Morphometric Landmark Detection
Authors: Nguyen Hoang Ha , Ho Bich Hai , Lai Hien Phuong , Tran Hoang Tung , Le Huu Ton , Banuls Anne - Laure , Prudhomme Jorian
Published: December 2021

Geometric morphometrics has become an important approach in insect morphology studies because it capitalizes on advanced quantitative methods to analyze shape. Shape could be digitized as a set of landmarks from specimen images. However, the existing tools mostly require manual landmark digitization, and previous works on automatic landmark detection methods do not focus on implementation for end-users. Motivated by that, we propose a novel approach for automatic landmark detection, based on visual features of landmarks and keypoint matching techniques. While still archiving comparable accuracy to that of the state-of-the-art method, our framework requires less initial annotated data to build prediction model and runs faster. It is lightweight also in terms of implementation, in which a four-step workflow is provided with user-friendly graphical interfaces to produce correct landmark coordinates both by model prediction and manual correction. The utility iMorph is freely available at https://github.com/ha-usth/WingLanmarkPredictor, currently supporting Windows, MacOS, and Linux.
Ecological Efficiency Evaluation and Spatiotemporal Characteristics Analysis of the Coupling Coordination of the Logistics Industry and Manufacturing Industry
Authors: Su Juan , Shen Tong , jin shuxin
Published: December 2021

The coupling coordination of the logistics industry and manufacturing industry conducive to the sustainable development of logistics and manufacturing and the stability of sustainable supply chain. The logistics and manufacturing industries are not only the basic industries that support social development, but also the industries with high carbon emissions. Firstly, this paper classifies the carbon emissions from the logistics industry and manufacturing industry as undesirable outputs, evaluates the ecological efficiency of the logistics industry (LEE) and manufacturing industry (MEE) in the Yangtze River Delta from 2006 to 2019 by using the unexpected slacks-based measure (SBM) model. Secondly, the coupling coordination method is used to analyze the coupling coordination scheduling of industrial ecological efficiency. Thirdly, the paper analyzes the spatial differences of the coupling coordination ecological efficiency between logistics industry and manufacturing industry (MLCC) by using the exploratory spatial data analysis method. Finally, the spatial econometric model is used to analyze the driving factors of the MLCC. The results show: The ecological efficiency of the manufacturing industry has steadily improved. The ecological efficiency of the logistics industry presents the rising trend in fluctuation. The level of the coupling coordination development between the logistics and manufacturing industries is high. The results of the spatial heterogeneity analysis show that the spatial differentiation of high-high agglomeration and low-low agglomeration is obvious. The spatial agglomeration characteristics are relatively stable, and the spatial diffusion effect is strong; In space, the MLCC shows a trend of developing from multiple agglomeration areas to one agglomeration area. The results of driving factor analysis show that foreign direct investment(FDI), government intervention(GI) and human capital(HP) have positive effects on the MLCC, while industrial structure(IS), environmental regulation(ER) and energy intensity(EI) have negative effects on the MLCC.
The Relationship Between Delirium and Statin Use According To Disease Severity in Patients in the Intensive Care Unit
Authors: An Jun Yong , Park Jin Young , Cho Jaehwa , Kim Hesun Erin , Park Jaesub , Oh Jooyoung
Published: December 2021

Background

There have been few earlier studies on the efficacy of statins in the prevention of delirium. However, the results were controversial. The aim of this study was to investigate the association between the use of statins and the occurrence of delirium in a large cohort of patients in the intensive care unit (ICU), considering disease severity and statin properties, which were not sufficiently considered in the previous works.
Methods

We obtained clinical and demographical information from 3604 patients admitted to the ICU of Gangnam Severance Hospital from January 2013 to April 2020. This included information on daily statin use and delirium state, as assessed by the Confusion Assessment Method for ICU. We used inverse probability of treatment weighting and categorized the ICU patients into four groups based on the Acute Physiology and Chronic Health Evaluation II score (group 1: 0-10 - mild; group 2: 11-20 – mild to moderate; group 3: 21-30 – moderate to severe; group 4: > 30 - severe). We analyzed the association between the use of statin and the occurrence of delirium in each group, while taking into account the properties of statins.
Results

Comparisons between statin and non-statin patient groups revealed that only in group 2, patients who were administered statin showed significantly higher occurrence of delirium (p=0.004, odds ratio [OR]=1.58) compared to the patients who did not receive statin. Regardless of whether statins were lipophilic (p=0.036, OR=1.47) or hydrophilic (p=0.032, OR=1.84), the occurrence of delirium was higher only in patients from group 2. Although both lipophilic and hydrophilic statins in group 2 were associated with delirium, neither showed a greater association than the other.
Conclusions

The use of statins may be associated with the increases in the risk of delirium occurrence in patients with mild to moderate disease severity, irrespective of statin properties, as revealed by results from a large cohort study.
Magnetospheric Convection: From Mesoscale Flows To Microscale Instabilities
Authors: Ukhorskiy Aleksandr Y. , Sorathia Kareem A. , Merkin Viacheslav G. , Crabtree Chris , Fletcher Alex C. , Malaspina David M. , Schwartz Steven J.
Published: December 2021

Plasma convection in the Earth’s magnetosphere from the distant magnetotail to the inner magnetosphere occurs largely in the form of mesoscale flows, i.e., discrete enhancements in the plasma flow with sharp dipolarizations of magnetic field. Recent spacecraft observations suggest that the dipolarization flows are associated with a wide range of kinetic processes such as kinetic Alfvén waves, whistler chorus waves, and nonlinear time-domain structures. In this paper we explore how mesoscale dipolarization flows produce suprathermal electron instabilities, thus providing free energy for the generation of the observed kinetic waves and structures. We employ three-dimensional test-particle simulations of electron dynamics one-way-coupled to a global magnetospheric model. The simulations show a rapid growth of interchanging regions of parallel and perpendicular electron temperature anisotropies distributed along the magnetic terrain formed around the dipolarization flows. Unencumbered in test-particle simulations, a rapid growth of velocity-space anisotropies in the collisionless magnetotail plasma is expected to be curbed by the generation of plasma waves. The results are compared with in situ observations of an isolated dipolarization flow at one of the spacecraft of the Magnetospheric Multiscale Mission, that show strong VLF wave activity alternating between broad-band wave activity and whistler waves. With estimated spatial extent being similar to the characteristic size of temperature anisotropy patches in our test-particle simulations, the observed bursts of VLF wave activity are likely to be produced by the parallel and perpendicular electron energy anisotropies driven by the dipolarization flow, as suggested by our results.
Development and Validation of a Novel M6A-related LncRNA Prognostic Signature Associated with Immune Microenvironment for Ovarian Serous Cystadenocarcinoma
Authors: Geng Rui , Chen Tian , Zhong Zihang , Ni Senmiao , Bai Jianling , Liu Jinhui
Published: December 2021

Background: OV is the most lethal gynecological malignancy. M6A and lncRNAs have great influence on OV development and patients' immunotherapy response. Here, we decided to establish a reliable signature in the light of mRLs. Method: The lncRNAs associated with m6A in OV were analyzed and obtained by co-expression analysis in the light of TCGA-OV database. Univariate, LASSO and multivariate Cox regression analyses were employed to establish the model in the light of the mRLs. K-M analysis, PCA, GSEA, and nomogram based on the TCGA-OV and GEO database were conducted to prove the predictive value and independence of the model. The underlying relationship between the model and TME and cancer stemness properties were further investigated through immune features comparison, consensus clustering analysis, and Pan-cancer analysis.Results: A prognostic signature comprising four mRLs: WAC-AS1, LINC00997, DNM3OS, and FOXN3-AS1, was constructed and verified for OV according to TCGA and GEO database. The expressions of the four mRLs were confirmed by qRT-PCR in clinical samples. Applying this signature, people can identify patients more effectively. All the sample were assigned into two clusters, and the clusters had different overall survival, clinical features, and tumor microenvironment. Finally, Pan-cancer analysis further demonstrated the four mRLs significantly related to immune infiltration, TME and cancer stemness properties in various cancer types. Conclusion: This study provided an accurate prognostic signature for patients with OV and elucidated the potential mechanism of the mRLs in immune modulation and treatment response, giving new insights into identifying new therapeutic targets.
METHANE LIQUEFACTION: Selective Conversion of Methane to Cyclohexane via Efficient Surface-Hydrogen-Transfer Catalyzed by GaN-Supported Platinum Clusters
Authors: Tan Li-Da , Su Hui , Han Jingtan , Li Mingxin , Li Chao-Jun
Published: December 2021

Non-oxidative liquefaction of methane at room temperature and ambient pressure has long been a scientific “holy grail” of chemical research. In this report, we exploit an unprecedented catalytic transformation of methane exclusively to cyclohexane through effective surface-hydrogen-transfer (SHT) at the heterojunctions boundary consisting of electron-rich platinum cluster (Pt) loaded on methane-activating gallium nitride (GaN) host. The experimental analysis demonstrates that interface-induced overall reaction starts with methane aromatization to benzene initiated by the Ga-N pairs, followed by hydrogenation of benzene to cyclohexane via hydrogen transfer. The in-situ activated hydrogen at electron-rich metal Pt cluster plays a key role for the hydrogenation and enables an outstanding selectivity (as high as 89 %) towards cyclohexane, which is well-delivered even after 5 recycling runs.
Young Stroke Secondary To Fibromuscular Dysplasia Causing Carotid Artery Dissection
Authors: Ranasinghe Rumesh , Azher Shifa , Ralapanawa Udaya , Karunathilake Parackrama
Published: December 2021

Background: Fibromuscular dysplasia (FMD) is a rare, medium-sized arteriopathy affecting less than 7% of the population. It affects predominantly young women of childbearing age. Stroke caused by fibromuscular dysplasia of the carotid artery is uncommon and reported to range between 0.6% by angiography and 1.1% by autopsy. Case Presentation: A 35-year-old mother of two children, previously well, was admitted to our hospital with right-sided spastic hemiparesis with upper motor neuron type facial nerve palsy. Non-contrast CT (NCCT) brain on admission revealed left-sided middle cerebral arterial territory infarction with possible hemorrhagic transformation with midline shift. She had progressive neurological features where NCCT brain in 24 hours showed an increase in the size of the hemorrhage. Magnetic resonance imaging (MRI) of the brain, including angiogram of neck vessels, revealed acute dissection of the left internal carotid artery with acute thrombosis, left frontoparietal hemorrhagic infarction, and the constellation of MRI showed features suggestive of left cervico-cranial fibromuscular dysplasia complicated by acute internal carotid artery dissection. She was started on low-dose aspirin, and 40 mg of atorvastatin and anticoagulants were not started because of the bleeding risk as she had a hemorrhagic transformation infarction. She was discharged and referred for inward physiotherapy and planned to repeat the MRI brain with the neck vessel angiogram in three weeks and arranged digital subtraction angiography in three months. Unfortunately, she was lost for follow-up.Conclusion: Detailed evaluation of the young patients with ischaemic stroke is helpful to diagnose rare diseases such as FMD involving carotid arteries leading to a better treatment choice between anticoagulation vs. antiplatelet therapy.
A Convenient Approximation of the Projectile Motion with Quadratic Air Resistance
Authors: Landon Marc
Published: December 2021

A convenient approximated analytic solution is proposed for the problem of the motion of a body under a resistive force, acting in the magnitude of the squared velocity of the body. This solution is an explicit function of time, that keeps a good behavior both near the initial state and far from the initial state.

To obtain a general analytic solution, we firstly used a reduction principle to be able to manipulate scalar objects, and we analyzed limit behaviors, both near the initial state and far from the initial state. Secondly, we proposed an approximated analytic solution with heuristics based on the built knowledge.

Finally, a robust and stable integration scheme is proposed, based on the obtained analytic solution. We compared the scheme with other standard integration schemes.
A COMPARATIVE CLINICAL EVALUATION FOR PRECISE ISOLATION USING ADDITION SILICONE DAM COVERAGE WITH CONVENTIONAL ISOLATION TECHNIQUES- AN IN-VIVO STUDY
Authors:   Postgraduate, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India., Suman Shradha , Lokhande Prakash ,   Proffessor, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India., N.M Dhanyakumar ,   Proffessor, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India., V. Siddheswaran ,   Proffessor, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India., Maria James Aneena ,   Postgraduate, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India., V. Vaipana Nayana ,   Postgraduate, Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India.
Published: December 2021

Context: A proper isolation technique plays a key role in the success of restoration of carious tooth. Aim Of The Study: The aim of this in-vivo study was to evaluate efficacy of addition silicone dam coverage technique with conventional isolation methods in restorative procedures and endodontic treatment. Settings And Design: Forty-Five patients who fulfill the inclusion criteria were selected for the study. Materials And Methods: All the participants underwent clinical procedures using 3 different isolation techniques. Group-1: Clinical procedures were carried out using traditional cotton roll isolation. Group-2: Clinical procedures were carried out using conventional rubber dam isolation procedure. Group-3: Clinical procedures were carried out using addition silicone dam coverage technique. The efficacy of isolation in terms of clinician usage and patient comfort is evaluated by a single evaluator. The results were tabulated and statistically analyzed. Statistical Analysis: IBM SPSS (version 21.0) software was used. Chi-square test was performed, considering P < 0.05 for statistical significance. Results: All the groups demonstrated satisfactory clinical performance. Upon inter and intra-group comparison of the isolation methods, there was statistically significant difference (P > 0.05). Conclusion: It is important to achieve an aseptic environment in clinical restorative and endodontic procedures. Addition silicone dam coverage technique is a chair side modified design which is user friendly with improved treatment efficacy. However, long-term clinical studies must be needed for further evaluation.
A STUDY ON INVESTORS PERCEPTION TOWARDS LARGE-CAP EQUITY ORIENTED MUTUAL FUNDS IN INDIA
Authors:   Assistant Professor, Dept. of P. G. Studies in Commerce, Vijayanagara Sri Krishnadevaraya University, Post Graduate Centre, Koppal Karnataka (India) - 583 231., Yalavatti Prakash
Published: December 2021

The large-cap equity fund is one of the mutual fund schemes and fund mobilized under this scheme is invested in equity securities of large-capitalized companies. This scheme is highly preferred scheme for investment by those who want to receive reasonably high return with less risk. The present study analyzes the profile, perception and satisfaction level of retail investors in large-cap equity funds. This study is based on both primary and secondary data. The study concludes that the majority of the investors are mid-aged people and men investors are more than women. Most of the investors have graduation and belong to the small saving group. The investors have moderately satisfied with large-cap fund investment on an average basis.
PHASE TRANSITION STUDIES OF CHOLESTERYL PALMITATE, CHOLESTERYL DECANOATE AND THEIR MIXTURES USING DIELECTRIC, EPR AND DTA TECHNIQUES
Authors:   Department of Physics, B.S.N.V.P.G.College, Lucknow, 226001, U.P., India., Misra Sanjai
Published: December 2021

The phase transition of two liquid crystalline materials, namely cholesteryl palmitate and cholesteryl decanoate and their mixtures (by weight)are studied using dielectric, Electron paramagnetic resonance (EPR) and differential thermal analysis (DTA) techniques. From the dielectric measurements, the phase transition temperatures were determined from the discontinuities in the curve between dielectric constant and temperatures for all the samples investigated. Further, since mixing of two liquid crystals modifies the transition of phases, the measurements were carried out on the mixtures of the two liquid crystals. Order parameter for each sample was calculated at varying temperatures by measuring the hyperfine splitting of three line EPR spectrums. The discontinuities in the curve between order parameter and the temperature again indicated the phase transition of the liquid crystals and their mixtures. A DTA study on these samples was also carried out in order to probe further their transitional behaviour. The DTA records of both the liquid crystals and their mixtures support the transition behaviour observed from the dielectric and EPR measurements.
A STUDY TO ASSESS THE EFFECTIVENESS OF PRAYANAMA TO RELIEVE STRESS AMONG THE PEOPLE OF SELECTED AREAS OF AHMEDABAD
Authors:   Principal, Apollo Institute of Nursing, Gandhinagar, Gujarat, India., Mohan Singh Hari , Patel Vijesh ,   Assistant Professor, Apollo Institute of Nursing, Gandhinagar, Gujarat, India., C Group ,   Students, Apollo Institute of Nursing, Gandhinagar, Gujarat, India.
Published: December 2021

Aim: To assess the effectiveness of pranayama to relieve stress at selected areas. Methodology: study design: one group pre-test postâ€“test research design1. Place and duration of study: The study was conducted in selected areas of Ahmedabad. The rationale for selecting this group people are the familiarity with the setting, locality, availability of sample, feasibility of conducting the study, easy accessibility of the samples. Data was collected from /08/2021 to /08/2021. Sample: Purposive sampling technique was used to select 30 samples at selected areas of Ahmedabad. Perceived stress scale was used to assess this stress level among the people. Results: The result shows that there is no significant effect on stress after pranayama .This study therefore offers an encouraging. The result might be achieved if the duration to the practice pranayama can be increased. Conclusion: The study reveals that the effect of pranayama on the suffering with stress has minimal effect. A person with mild stress encompasses improvement in condition.
INVESTIGATION OF HEAT TRANSFER ON TWO ADJACENT NARROW PLATES WITH NATURAL CONVECTION
Authors:   PG Scholar, Department of Mechanical Engineering, Navodaya Institute of Technology, Raichur, Karnataka-584103, India., Aamir Sohail Md. , Kulkarni H. Ravi ,   Department of Mechanical Engineering, Navodaya Institute of Technology, Raichur, Karnataka-584103, India., Rathnakumar P. ,   Department of Mechanical Engineering, Navodaya Institute of Technology, Raichur, Karnataka-584103, India., Akthar Faheem ,   Department of Mechanical Engineering, Navodaya Institute of Technology, Raichur, Karnataka-584103, India., Ali Zeeshan ,   Department of Mechanical Engineering, Navodaya Institute of Technology, Raichur, Karnataka-584103, India., Nagaral Mahadev ,   Aircraft Research and Design Centre, HAL, Bengaluru, India.
Published: December 2021

Natural convection flow in a vertical channel containing inside objects exists in a number of advance technological applications, including heat transfer from electronic circuits, refrigerators, heat exchangers, power station, fueling components, chillers, and residential ventilation, etc. In this thesis the model is created in CREO, and then imported into ANSYS to simulate air flow through vertical thin plates. The focus of the thesis will be on thermal and CFD modeling of vertical thin plates with variable Reynolds numbers (2Ã—106& 4Ã—106) and at angles (0Â°,30Â°&60Â°) respectively. Thermal analysis was performed on vertical thin narrow plates made up of steel, aluminium, and copper at various heat transfer coefficient rates.Modeling and analysis of the narrow vertical plate is demonstrated utilising the data books details and design formulary.CFD analysis results tabulated at different Reynolds number, pressure, velocity, heat transfer coefficient, mass flow rate and heat transfer and also which is drawn into graphical representation.3D modelling was done in the parametric application Pro-Engineer, and analysis is performed in ANSYS.