bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Comparative Review of the Cement Experimental Testing Under CO2 Conditions
Authors: Abid Khizar , Baena Velásquez Andrés Felipe , Teodoriu Catalin
Published: November 2024

Global warming is presently one of the most pressing issues the planet faces, with the emission of greenhouse gasses being a primary concern. Among these gasses, CO2 is the most detrimental because, among all the greenhouse gasses resulting from anthropogenic sources, CO2 currently contributes the largest share to global warming. Therefore, to reduce the adverse effects of climate change, many countries have signed the Paris Agreement, according to which net zero emissions of CO2 will be achieved by 2050. In this respect, Carbon Capture and Sequestration (CCS) is a critical technology that will play a vital role in achieving the net zero goal. It allows CO2 from emission sources to be injected into suitable subsurface geological formations, aiming to confine CO2 underground for hundreds of years. Therefore, the confinement of CO2 is crucial, and the success of CCS projects depends on it. One of the main components on which the confinement of the CO2 relies is the integrity of the cement. As it acts as the barrier that restricts the movement of the sequestrated CO2 to the surface. However, in a CO2-rich environment, cement reacts with CO2, leading to the deterioration of its physical, chemical, transfer, morphological, and mechanical properties. This degradation can create flow paths that enable the leakage of sequestered CO2 to the surface, posing risks to humans, animals, and the environment. To address this issue, numerous studies have investigated the use of various additives in cement to reduce carbonation, thus enhancing the cement’s resistance to supercritical (sc) CO2 and maintaining its integrity. This paper provides a comprehensive review of current research on cement carbonation tests conducted by different authors. It includes detailed descriptions of the additives used, testing setups, curing conditions, methodologies employed, and experimental outcomes. This study will help to provide a better understanding of the carbonation process of the cement sample exposed to a CO2-rich environment, along with the pros and cons of the additives used in the cement. A significant challenge identified in this research is the lack of a standardized procedure for conducting carbonation tests, as each study reviewed employed a unique methodology, making direct comparisons difficult. Nonetheless, the paper provides an overview of the most commonly used temperatures, pressures, curing durations, and carbonation periods in the studies reviewed.
Sustainable Charging Stations for Electric Vehicles
Authors: Armenta-Déu Carlos , Sancho Luis
Published: November 2024

In this work, we develop a detailed analysis of the current outlook for electric vehicle charging technology, focusing on the various levels and types of charging protocols and connectors used. We propose a charging station for electric cars powered by solar photovoltaic energy, performing the analysis of the solar resource in the selected location, sizing the photovoltaic power plant to cover the demand completely, and exploring different configurations such as grid connection or physical and virtual electric energy storage. Despite the current development applying for specific working conditions, operating voltage, charging rate, power demand, etc., the proposed configuration is modular, adaptable, and resilient. The simulated system operates within the 360 V to 800 V range of direct current for charging the electric vehicles, with a selectable power range between 20 and 180 kW. The basic layout includes four charging poles, each servicing all working voltages. An oversized PV plant powers the charging station at any time of the year, saving money compared to the alternative of the electric storage unit. In addition, we build simulation tools and algorithms that optimize the design of future projects, providing a solid basis for sustainable energy infrastructure planning and design.
Effect of Acid Production from Forest Litter on the Availability of Heavy Metals in Soil
Authors: Zhang Yujie , Zhou Jiangmin , Lei Zupei , Ren Han , Chen Hualin
Published: November 2024

Forest leaf litter is an important source of soil nutrients, but how its decomposition products affect the availability of soil heavy metals is not totally clear. In order to understand the effects of leaf litter decomposition on soil heavy metal availability in a forest ecosystem, leaf litter samples were collected from Daluoshan in Wenzhou and the Wuyanling National Nature Reserve of Zhejiang and subjected to analysis. The leaf litter was produced by the forests’ dominant tree species, such as Myrica ruba, Bambusa cerosissima, Pinus sylvestris, Machilus thunbergia, Cunninghamia lanceolata, and Quercus acutissima. Decomposition of the leaf litter samples at room temperature and leaching decomposition of the samples were carried out to analyze the acid production characteristics of the litter samples and their impact on soil heavy metal availabilities. Oxalic acid, lactic acid, fumaric acid, succinic acid, malic acid, and citric acid were the dominant organic acids in the leaf litter decomposer, and the sum of these six organic acids accounted for more than 50% of the total organic acid. During a 30-day litter decomposition, the levels of organic acids basically increased, with significant increases appearing in the early stage. After leaching, the available contents of Cr, Mn, Ni, Cu, Zn, As, Cd, and Pb increased by a maximum of 11.95, 2.33, 12.00, 0.80, 0.83, 0.54, 51.50, and 0.28-fold, respectively, compared with those of the original soil sample. During leaching, the higher the content of low molecular weight organic acids in the leaf litter, the longer the leaching time required and the more obvious the influence on the level of available heavy metals. A stronger leaching effect was found for the soil samples taken from a depth of 0–20 and 20–40 cm accompanied by a more obvious increase in the available heavy metals in the soil. PLSPM results showed that there were significant differences between the soil acidity index and the available heavy metals in Daluoshan (p < 0.05) and that there were significant differences between the soil acidity index and the available heavy metals in Wuyanling (p < 0.01). The decomposition of litter produced low molecular weight organic acids, which caused a decrease in soil pH and an increase in exchangeable H+ and Al3+. Both changes had an impact on soil organic matter and further led to an increase in the activity of heavy metals in the soil. This will further lead to the increase of ecological and environmental risks to forest soil.
Improving Propagation Efficiency of Eucalyptus cloeziana ‘Chuanlinzhen 7523’ by Optimizing Explants and Proliferation Medium
Authors: Lin Zihao , Dong Yang , Su Xinyao , Chen Zhi , Masabni Joseph , Huang Zhen , Xiong Huan
Published: November 2024

Eucalyptus cloeziana is an important, fast-growing, precious timber species in southern China, with tissue culture being the primary method for its propagation. However, the declining proliferation coefficient of adventitious shoots with multi-generation culture is a major constraint on its rapid propagation. This study aims to address this issue through the selection of suitable explants and optimizing the plant growth regulators’ formulation during the process of shoot proliferation. In this study, we cut shoots from the 21st generation of the tissue-cultured seedlings of ‘Chuanlinzhen 7523’ into apical, middle, and basal sections, and we measured their content of endogenous hormones. The proliferation coefficient (PC) and growth coefficient (GC) of explants under different concentrations of plant growth regulators were analyzed and ranked using the PCA method. The results indicated that the shoot basal sections were the best for proliferation when treated with 0.40 mg/L BAP (6-benzylaminopurine), 0.20 mg/L NAA (naphthaleneacetic acid), and 0.20 mg/L TDZ (thidiazuron), yielding a PC of 4.7 and a GC of 4.1. These basal sections ranked first with a rooting rate of up to 80%. When the rooted plantlets were acclimatized in the nursery, a survival rate of 100% was achieved. This protocol—from proliferation to acclimation—effectively improves the propagation efficiency of E. cloeziana ‘Chuanlinzhen 7523’ after multi-generation propagation.
The Effect of a Nature-Based Gel on Gingival Inflammation and the Proteomic Profile of Crevicular Fluid: A Randomized Clinical Trial
Authors: Figueiredo Luciene Cristina , Bueno-Silva Bruno , Denúncio Giovanna , Figueiredo Nathalia Freitas , Cruz Daniele Ferreira da , Shibli Jamil A. , Borges Maria Helena R. , Barão Valentim A. R. , Haim Doron , Asbi Thabet , Souza João Gabriel S.
Published: November 2024

Evidence has shown the clear positive effects of nature-based products on biofilm control and improved gingival health. However, most studies have used in vitro models, have tested single natural components, or have not evaluated proteomic changes after treatment. This double-blind, parallel, randomized, and controlled clinical trial evaluated the benefits of a nature-based gel in controlling gingival inflammation and its effects on the proteomic gingival crevicular fluid (GCF) profile. Gingivitis patients were distributed into the following groups: (1) nature-based gel containing propolis, aloe vera, green tea, cranberry, and calendula (n = 10); (2) control—conventional toothpaste (n = 10). GCF was collected and evaluated by means of liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). At 3 months, the groups showed similar clinical benefits (p < 0.05). A total of 480 proteins were identified across all groups. In a pooled comparison of both groups at both time points, exclusive proteins were identified in the nature-based gel (78) and the control (21) groups. The exclusive proteins identified for the toothpaste mainly acted in wound healing, and those for the nature-based gel mainly acted on immune system processes. The nature-based gel achieved similar clinical outcomes to conventional toothpaste. However, the nature-based gel markedly changed the proteomic profile of GCF after treatment, showing a profile associated with a host response.
The Chicken HDAC4 Promoter and Its Regulation by MYC and HIF1A
Authors: Wang Yingjie , Kong Ruihong , Xie Ke , Wang Xu , Wu Han , Zhang Yani
Published: November 2024

Background: Histone deacetylase 4 (HDAC4) is a member of the class II histone deacetylase family, whose members play a crucial role in various biological processes. An in-depth investigation of the transcriptional characteristics of chicken HDAC4 can provide fundamental insights into its function. Methods: We examined HDAC4 expression in chicken embryonic stem cells (ESC) and spermatogonial stem cells (SSC) and cloned a 444 bp fragment from upstream of the chicken HDAC4 transcription start site. Subsequently, we constructed pEGFP-HDAC4 and a series of 5′-deletion luciferase reporter constructs, which we transfected into DF-1 cells to measure their transcriptional activity. The regulatory mechanisms of chicken HDAC4 expression were investigated by performing trichostatin A (TSA) treatment, deleting putative transcription factor binding sites, and altering transcription factor expression levels. Results: HDAC4 exhibited higher expression in SSC than in ESC. We confirmed that the upstream region from −295 bp to 0 bp is the core transcriptional region of HDAC4. TSA effectively inhibited HDAC4 transcription, and bioinformatics analysis indicated that the chicken core HDAC4 promoter sequence exhibits high homology with those of other avian species. The myelocytomatosis viral oncogene homolog (MYC) and hypoxia-inducible factor 1 α (HIF1A) transcription factors were predicted to bind to this core region. Treatment with TSA for 24 h resulted in the upregulation of MYC and HIF1A, which repressed HDAC4 transcription. Conclusions: Our results provide a basis for subsequent investigations into the regulation of HDAC4 expression and biological function.
Forensic STR Loci and Schizophrenia: An Exploration of Implications for Forensic Applications and Genetic Privacy
Authors: Yang Qi , Yang Chun , Hua Zhiqi , Shen Qi , Chen Anqi , Ba Huajie , Zhang Suhua
Published: November 2024

Background/Objectives: Short tandem repeat (STR) loci are widely used in forensic genetics for identification and kinship analysis. Traditionally, these loci were selected to avoid medical associations, but recent studies suggest that loci such as TH01 and D16S539 may be linked to psychiatric conditions like schizophrenia. This study explores these potential associations and considers the privacy implications related to disease susceptibility. Methods: We analyzed 19 STR loci, including CODIS core loci and additional loci like Penta D and Penta E. Statistical analyses were conducted on a dataset of schizophrenia patients and matched control individuals to assess the relationship between STR polymorphisms and schizophrenia risk. Results: No significant associations were found between the 19 analyzed loci and schizophrenia in this dataset. While initial analyses revealed minor allele frequency differences at the D3S1358, D13S317, and TPOX loci between the schizophrenia and control groups, these differences did not retain statistical significance following Bonferroni correction (corrected p < 0.0026 for all loci). Conclusions: Although no significant associations were found between STR loci and schizophrenia, this study highlights the importance of considering the potential for forensic DNA data to reveal health-related information. As forensic DNA databases continue to expand, there is a growing need to reassess ethical and legal guidelines to ensure the protection of individual privacy. Future research should continue exploring these genetic associations with larger, more diverse samples to further understand their implications.
The Role of 11C-Methionine PET Imaging for the Evaluation of Lymphomas: A Systematic Review
Authors: Dondi Francesco , Gazzilli Maria , Viganò Gian Luca , Pisani Antonio Rosario , Ferrari Cristina , Rubini Giuseppe , Bertagna Francesco
Published: November 2024

Background: In the last years, different evidence has underlined a possible role for [11C]-methionine ([11C]MET) positron emission tomography (PET) imaging for the evaluation of lymphomas. The aim of this paper was, therefore, to review the available scientific literature focusing on this topic. Methods: A wide literature search of the PubMed/MEDLINE, Scopus and Cochrane Library databases was conducted in order to find relevant published articles investigating the role of [11C]MET in the assessment of lymphomas. Results: Eighteen studies were included in the systematic review and the main fields of application of this imaging modality were the evaluation of disease, therapy response assessment, prognostic evaluation and differential diagnosis with other pathological conditions. Conclusion: Even with heterogeneous evidence, a possible role for [11C]MET PET imaging in the assessment of lymphomas affecting both the whole body and the central nervous system was underlined. When compared to [18F]fluorodesoxyglucose ([18F]FDG) imaging, in general, similar results have been reported between the two modalities in these settings.
Cryopreservation of Lavender Trumpet Tree (Handroanthus impetiginosus) Seeds
Authors: Campos Thiago Souza , Pereira Vania M. , El Merzougui Soumaya , Beleski David , Pérez Héctor E. , Pivetta Kathia Fernandes Lopes , Vendrame Wagner A.
Published: November 2024

In response to the near-threatened status of Handroanthus impetiginosus, primarily due to habitat loss and illegal logging, this study examines how X-ray imaging and cryopreservation impact the seed quality and viability essential for conservation. Seeds initially had a moisture content of 12.3%, reduced to 6.5% through desiccation. X-ray imaging allowed for detailed visualization of internal structures, identifying seeds as normal, abnormal, or dead based on damage and development. Normal seeds consistently germinated and produced healthy seedlings, while those with internal damage or excessive desiccation either resulted in abnormal seedlings or did not germinate. Various cryopreservation treatments were tested, including storage at −80 °C and liquid nitrogen immersion (LN), with and without vitrification solutions (PSV2; PVS3; PSV2 + 1% phloroglucinol; PSV3 + 1% phloroglucinol). Results indicated that immersion in LN without cryoprotectants achieved the highest germination and seedling viability, whereas vitrification solutions, such as PVS2 and PVS3, negatively affected germination. This study demonstrates that X-ray imaging is an effective tool for assessing seed quality and detecting internal damage, while cryopreservation without cryoprotectants is suitable for long-term seed storage. This work highlights the benefits of combining X-ray assessment with optimized cryopreservation techniques to support the conservation of threatened species.
Occupational Risk Factors for Burnout Syndrome Among Healthcare Professionals: A Global Systematic Review and Meta-Analysis
Authors: Amiri Sohrab , Mahmood Nailah , Mustafa Halla , Javaid Syed Fahad , Khan Moien AB
Published: November 2024

Health professionals are disproportionately affected by burnout compared to other occupational groups. This study aims to systematically review and meta-analyze thirteen occupational risk factors related to burnout syndrome among health professionals globally. A comprehensive literature search was conducted in August 2023. The protocol was registered in The International Prospective Register of Systematic Reviews (PROSPERO), registration number CRD42023396081. Using a random-effects model, this meta-analysis assessed the association between occupational risk factors and burnout, reporting odds ratios (ORs) and 95% confidence intervals (CIs). The meta-analysis included 109 studies from diverse global locations. Key factors influencing burnout included workplace bullying, job stress, and poor communication, with protective factors such as supportive work environments, adequate staffing, and individual resilience. All risk factors examined showed a significant positive relationship with burnout incidence. Workplace bullying was strongly associated with increased burnout (OR 4.05–15.01, p < 0.001). Similarly, low job satisfaction and high job stress were strongly associated with burnout, with ORs of 5.05 (95% CI 3.88–6.56, p < 0.001) and 4.21 (95% CI 1.62–10.94, p = 0.003), respectively. The review findings highlight the importance of addressing these risk factors through enhanced supportive work environments and promoting personal resilience strategies.
BAMBI Is a Prognostic Biomarker Associated with Macrophage Polarization, Glycolysis, and Lipid Metabolism in Hepatocellular Carcinoma
Authors: Gao Huijie , Hu Cuimin , Wu Qing , Fang Zhongze
Published: November 2024

Hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide. Affected patients have poor prognoses due to high rates of post-surgical recurrence and metastasis. Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) reportedly contributes to the development and progression of various human cancers. Thus far, there have been no comprehensive studies regarding the expression of BAMBI in HCC; similarly, no studies have investigated the prognostic significance of BAMBI and its associated mechanisms in HCC. In this study, we analyzed the expression profiles of BAMBI, along with its contributions to pathological findings, metastasis characteristics, and prognosis, in multiple human cancers. We found that upregulation of BAMBI was associated with poor prognosis in HCC. Next, we explored the associations of BAMBI with multiple cell signaling pathways, immune cells, and immune checkpoints in HCC. The results showed that BAMBI was associated with tumor proliferation, epithelial–mesenchymal transition (EMT) markers, glycolysis, fatty acid biosynthesis and degradation pathways, and immune checkpoint regulation in HCC. In vitro and in vivo experiments showed that BAMBI promoted polarization of M1 macrophages and is linked to the expression of key genes involved in glycolipid metabolism. Furthermore, protein–protein interaction analysis suggested that BAMBI plays multiple roles in HCC by regulating genes in the transforming growth factor (TGF)-β and Wnt signaling pathways. Our findings elucidated that BAMBI is a prognostic biomarker and is associated with macrophage polarization, glycolysis, and lipid metabolism in HCC.
Exogenous Calcium Enhances Castor Tolerance to Saline–Alkaline Stress by Regulating Antioxidant Enzyme Activity and Activating Ca2+ and ROS Signaling Crosstalk
Authors: Hao Fei , Cui Zhigang , Dong Xuan , Gao Yan , Wang Rongjin , Zhang Hui , Lin Guolin
Published: November 2024

Saline–alkaline stress is a major factor limiting agricultural development, with calcium (Ca2+) playing a role in regulating plant tolerance through multiple signaling pathways. However, the specific mechanisms by which Ca2+ mediates saline–alkaline stress tolerance at the molecular level remain incompletely understood. This study investigates the effects of exogenous Ca2+ application on enhancing plant tolerance to saline–alkaline stress, focusing on its impact on the antioxidant system and Ca2+ and reactive oxygen species (ROS) signaling pathways. Through physiological assays and transcriptomic analyses, we evaluated oxidative damage markers, antioxidant enzyme activities, and the expression of key Ca2+ and ROS signaling genes. The results showed that saline–alkaline stress significantly elevated ROS levels, which led to increased membrane lipid peroxidation and induced upregulation of antioxidant response elements in castor roots. Exogenous calcium treatment reduced ROS accumulation by increasing superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities and decreasing malondialdehyde (MDA) levels, demonstrating a marked improvement in the antioxidant system. Transcriptomic analysis identified CAT2 (LOC107261240) as the primary target gene associated with increased CAT activity in response to exogenous calcium. Additionally, the upregulation of specific Ca2+ channels, Ca2+ sensors, ROS receptors, and antioxidant-related genes with calcium treatment highlights the critical role of Ca2+–ROS signaling crosstalk in enhancing stress tolerance. Protein–protein interaction analysis identified APX3 and other hub genes involved in Ca2+–ROS signaling transduction and the regulation of antioxidant activity. These findings enhance our understanding of calcium’s complex regulatory roles in plant abiotic stress responses, offering new theoretical insights for improving crop resilience in agriculture.
The DLEU2–miR-15a–16-1 Cluster Is a Determinant of Bone Microarchitecture and Strength in Postmenopausal Women and Mice
Authors: Reppe Sjur , Reseland Janne Elin , Prijatelj Vid , Prediger Michael , Nogueira Liebert Parreiras , Utheim Tor Paaske , Rivadeneira Fernando , Gautvik Kaare M. , Datta Harish Kumar
Published: November 2024

This study explores how select microRNAs (miRNAs) influence bone structure in humans and in transgenic mice. In trabecular bone biopsies from 84 postmenopausal women (healthy, osteopenic, and osteoporotic), we demonstrate that DLEU2 (deleted in lymphocytic leukemia 2)-encoded miR-15a-5p is strongly positively associated with bone mineral density (BMD) at different skeletal sites. In bone transcriptome analyses, miR-15a-5p levels correlated positively with the osteocyte characteristic transcripts SOST (encoding sclerostin) and MEPE (Matrix Extracellular Phosphoglycoprotein), while the related miR-15b-5p showed a negative association with BMD and osteoblast markers. The data imply that these miRNAs have opposite roles in bone remodeling with distinct actions on bone cells. Expression quantitative trait loci (eQTL) variants confirmed earlier DLEU2 associations. Furthermore, a novel variant (rs12585295) showed high localization with transcriptionally active chromatin states in osteoblast primary cell cultures. The supposition that DLEU2-encoded miRNAs have an important regulatory role in bone remodeling was further confirmed in a transgenic mice model showing that miR-15a/16-1-deleted mice had significantly higher percentage bone volume and trabecular number than the wild type, possibly due to prenatal actions. However, the three-point mechanical break force test of mice femurs showed a positive correlation between strength and miR-15a-5p/miR-16-5p levels, indicating differential effects on cortical and trabecular bone. Moreover, these miRNAs appear to have distinct and complex actions in mice prenatally and in adult humans, impacting BMD and microstructure by regulating bone cell transcription. However, detailed interactions between these miRNAs and their downstream mechanisms in health and disease need further clarification.
Drosophila: An Important Model for Exploring the Pathways of Inflammatory Bowel Disease (IBD) in the Intestinal Tract
Authors: Zeng Chuisheng , Liu Fengying , Huang Yuhan , Liang Qianqian , He Xiaohong , Li Lingzhi , Xie Yongfang
Published: November 2024

Inflammatory bowel disease (IBD) is a chronic and recurring lifelong condition, the exact etiology of which remains obscure. However, an increasing corpus of research underscores the pivotal role of cellular signaling pathways in both the instigation and management of intestinal inflammation. Drosophila, owing to its prodigious offspring, abbreviated life cycle, and the conservation of signaling pathways with mammals, among other advantages, has become a model organism for IBD research. This review will expound on the feasibility of utilizing Drosophila as an IBD model, comparing its intestinal architecture with that of mammals, its inflammatory responses, and signaling pathways. Furthermore, it will deliberate on the role of natural products across various biological models of IBD pathways, elucidating the viability of fruit flies as IBD models and the modus operandi of cellular signaling pathways in the context of IBD.
Frequencies of an IFNL4 Variant in an Admixed Population from Amazonia and Its Influence on Hepatitis C Infection
Authors: Angelim Carolina Cabral , Andrade Álesson Adam Fonseca , de Sousa Renata Santos , Correa Raissa Lima , Sacramento Amanda Roberta Vieira , Martins Letícia Dias , Conde Simone Regina Souza da Silva , Vallinoto Antonio Carlos Rosário , Feitosa Rosimar Neris Martins , Costa Greice de Lemos Cardoso
Published: November 2024

The rs12979860 polymorphism, related to the IFNL4 gene, is suggested as a factor that impacts fibrosis progression in hepatitis C virus (HCV) infection and exhibits a wide distribution pattern across global populations. In this retrospective cross-sectional study, we aimed to investigate the frequency of this variant in an Amazonian population from Brazil, as well as its association with liver fibrosis development and its staging in HCV carriers. Our results show a significant association of the TT genotype in the sample of patients with HCV (OR = 2.291; 95% CI = 1.088–4.826; p = 0.033) and the greater frequency of the T allele (62.1%), which is similar to the those of African populational groups. Populational genetics analysis showed significant differences in allele frequencies on global levels. The frequency of the C allele in the study population (37.8%) was like that of the African population (39.7%), and differed from all other populations, which ranged from 62.5% to 92.9%. These findings suggest that rs12979860 plays a role in susceptibility to hepatitis C. Additionally, they allow us to propose that the response to hepatitis C infection in this group may resemble that of the African population.
Redefining Roles: A Paradigm Shift in Tryptophan–Kynurenine Metabolism for Innovative Clinical Applications
Authors: Tanaka Masaru , Szabó Ágnes , Vécsei László
Published: November 2024

The tryptophan–kynurenine (KYN) pathway has long been recognized for its essential role in generating metabolites that influence various physiological processes. Traditionally, these metabolites have been categorized into distinct, often opposing groups, such as pro-oxidant versus antioxidant, excitotoxic/neurotoxic versus neuroprotective. This dichotomous framework has shaped much of the research on conditions like neurodegenerative and neuropsychiatric disorders, as well as cancer, where metabolic imbalances are a key feature. The effects are significantly influenced by various factors, including the concentration of metabolites and the particular cellular milieu in which they are generated. A molecule that acts as neuroprotective at low concentrations may exhibit neurotoxic effects at elevated levels. The oxidative equilibrium of the surrounding environment can alter the function of KYN from an antioxidant to a pro-oxidant. This narrative review offers a comprehensive examination and analysis of the contemporary understanding of KYN metabolites, emphasizing their multifaceted biological functions and their relevance in numerous physiological and pathological processes. This underscores the pressing necessity for a paradigm shift in the comprehension of KYN metabolism. Understanding the context-dependent roles of KYN metabolites is vital for novel therapies in conditions like Alzheimer’s disease, multiple sclerosis, and cancer. Comprehensive pathway modulation, including balancing inflammatory signals and enzyme regulation, offers promising avenues for targeted, effective treatments.
Efficient Genome Editing Using ‘NanoMEDIC’ AsCas12a-VLPs Produced with Pol II-Transcribed crRNA
Authors: Borovikova Sofiia E. , Shepelev Mikhail V. , Mazurov Dmitriy V. , Kruglova Natalia A.
Published: November 2024

Virus-like particles (VLPs) are an attractive vehicle for the delivery of Cas nuclease and guide RNA ribonucleoprotein complexes (RNPs). Most VLPs are produced by packaging SpCas9 and its sgRNA, which is expressed from the RNA polymerase III (Pol III)-transcribed U6 promoter. VLPs assemble in the cytoplasm, but U6-driven sgRNA is localized in the nucleus, which hinders the efficient formation and packaging of RNPs into VLPs. In this study, using the nuclease packaging mechanism of ‘NanoMEDIC’ VLPs, we produced VLPs with AsCas12a and exploited its ability to process pre-crRNA. This allowed us to direct crRNA in the cytoplasm as part of a Pol II-driven transcript where AsCas12a excised mature crRNA, thus boosting RNP incorporation into VLPs. CMV-driven crRNA increased Venus and CCR5 transgene knockout levels in 293 cells from 30% to 50–90% and raised the level of endogenous CXCR4 knockout in Jurkat T cells from 1% to 20%. Changing a single crRNA to an array of three or six identical crRNAs improved CXCR4 knockout rates by up to 60–70%. Compared to SpCas9-VLPs, the editing efficiencies of AsCas12a-VLPs were higher, regardless of promoter usage. Thus, we showed that AsCas12a and CMV-driven crRNA could be efficiently packaged into VLPs and mediate high levels of gene editing. AsCas12a-VLPs are a new and promising tool for the delivery of RNPs into mammalian cells that will allow efficient target genome editing and may be useful for gene therapy applications.
Large-Scale Synthesis of Carbon Dots Driven by Schiff Base Reaction at Room Temperature
Authors: Shi Jifen , Chang Shuai , Gao Yating , Lv Jian , Qian Ruocan , Chen Binbin , Li Dawei
Published: November 2024

Photoluminescent carbon dots (CDs) have received increasing attention because of their admirable photophysical performances. The current strategies for synthesizing CDs typically require high energy consumption levels, and the ability to synthesize CDs at ambient temperature would be highly desirable. Herein, we design an energy-efficient approach to synthesize CDs through a Schiff base crosslinking between 2,5-dihydroxy-1,4-benzoquinone and tetraethylenepentamine at room temperature. The obtained CDs possess maximum photoluminescence (PL) emissions of 492 nm. Moreover, the proposed CDs possess good stability and a concentration-dependent PL and their maximum emissions can redshift from 492 to 621 nm as the CDs concentration increases. Because of their good luminescent properties, the CDs can be employed as optical probes for doxorubicin detection using the inner filter effect. This study develops a powerful approach for the large-scale synthesis of CDs with a superior performance.
Benefits from Implementing Low- to High-Intensity Inspiratory Muscle Training in Patients Undergoing Cardiac Surgery: A Systematic Review
Authors: Evangelodimou Aphrodite , Patsaki Irini , Andrikopoulos Alexandros , Chatzivasiloglou Foteini , Dimopoulos Stavros
Published: November 2024

Cardiac surgery procedures are among the main treatments for people with cardiovascular disease, with physiotherapy playing a vital part. Respiratory complications are common and associated with prolonged Intensive Care Unit (ICU) and hospital stay, as well as increased mortality. Inspiratory muscle training has been found to be beneficial in improving respiratory muscle function in critically ill patients and patients with heart failure. The purpose of this review is to present the results of implementing inspiratory muscle training (IMT) programs in patients before and/or after cardiac surgery. The PubMed, Embase and Science Direct databases were searched from January 2012 to August 2023. In the present review, randomized controlled clinical trials (RCTs), clinical trials and quasi-experimental studies conducted in adult patients pre and/or post cardiac surgery were included. Fifteen studies were considered eligible for inclusion in the review. The results revealed that the IMT programs varied in intensity, repetitions, and duration in all included studies. Most studies implemented the IMT after the surgery. Statistical significance between groups was noted in Maximal Inspiratory Pressure and the 6-Minute Walk Distance Test. Preoperative and postoperative programs could improve inspiratory muscle strength, pulmonary function, and functional capacity as well as decrease the length of hospital stay in patients undergoing cardiac surgery. No clear evidence emerged favoring low or higher IMT intensities. The combination of IMT with other forms of exercise might be beneficial in patients undergoing cardiac surgery. However, further RCTs are required to provide confirming evidence.
Lateral Extraarticular Tenodesis in Revision Anterior Cruciate Ligament Reconstruction: An Analysis of Clinical Outcomes and Failure Rates
Authors: Fritsch Lorenz , Bausch Luca , Runer Armin , Winkler Philipp W. , Vieider Romed P. , Siebenlist Sebastian , Mehl Julian , Willinger Lukas
Published: November 2024

Background/Objectives: Lateral extraarticular tenodesis (LET) has been advocated in revision anterior cruciate ligament reconstruction (ACLR) to improve knee stability and furthermore, decrease failure rates. The aim of this study was to compare clinical outcomes, knee laxity, and failure rates after revision ACLR with LET (ACLR + LET) versus without LET. It was hypothesized that ACLR + LET improves clinical outcomes and reduces the failure rate. Methods: A retrospective analysis of prospectively collected data was conducted to examine patients who underwent revision ACLR with and without LET between 2017 and 2021 with a minimum follow-up of 24 months. Patients with coronal malalignment (>5°), posterior tibial slope >12°, and concomitant injuries to collateral ligaments were excluded. Patient reported outcome measures (PROMs) included the International Knee Documentation Committee (IKDC) subjective knee score, the Lysholm score, and the Tegner activity scale (TAS). Anterior knee laxity was measured with a Rolimeter and side-to-side difference (SSD) was determined. Revision ACLR failure was defined as ACL revision surgery and SSD > 5 mm. Group comparisons were performed using chi-square-, independent-samples students t-test or Mann–Whitney–U tests. Results: Of 56 eligible patients, 45 (80%, follow up, 23 isolated rACLR vs. 22 rACLR + LET) were included with a mean follow-up of 45.6 ± 15.8 months. Postoperative PROMs were not statistically different between rACLR and rACLR + LET groups (IKDC: 73.0 ± 18.9 vs. 68.7 ± 16.6, n.s.; Lysholm: 84.8 ± 12.3 vs. 77.7 ± 16.2, n.s.). Both groups showed similar TAS (rACLR vs. rACLR +LET (5; range 4–6 vs. 4; range 3–5; n.s.). Anterior knee laxity SSD was 2.4 ± 1.3 mm in the rACLR group and 1.8 ± 1.8 mm in the rACLR + LET group (n.s.). The failure rate was 13% in the rACLR group compared to 4.5% in the rACLR + LET group (n.s.). Conclusions: Isolated revision ACLR showed comparable postoperative patient-reported outcome measures and anterior knee laxity compared to ACLR + LET at mid-term follow up. The addition of a LET demonstrated a lower, though non-significant, failure rate after revision surgery. However, future studies with a prospective, randomized design and an increased number of patients are needed to clearly identify the exact indication for the use of additional LET.
Oxygenase Ppo-Regulated Moldy Volatiles Affect Growth, Pathogenicity and Patulin Biosynthesis of Penicillium expansum Through G Protein Signaling
Authors: Gong Di , Yan Tingting , Wang Xuexue , Prusky Dov , Long Danfeng , Zhang Ying , Bi Yang
Published: November 2024

Precocious sexual inducer (psi)-producing oxygenases (Ppos) participate in the production of C8 moldy volatile compounds (MVOCs), and these compounds could act as signal molecules modulating G protein signaling cascades, which participates in the growth and development, secondary metabolisms and pathogenicity of filamentous fungi. In this study, PePpoA and PePpoC proteins were identified in Penicillium expansum. The deletion of ppoA decreased C8 MVOC production in P. expansum, while they were not detected in the ΔppoC strain (p < 0.05). In addition, down-regulated cAMP/PKA and PKC/PLC signaling showed in the two mutants (p < 0.05). The two mutants showed slow colony growth and down-regulated expression of genes regulating spore development (abaA, wetA, brlA and vosA) with broken morphology of spore and hyphae. In addition, the two mutants had decreased pathogenicity on apple fruit and less patulin production in vitro and in vivo. Compared with ΔppoA strain, the deletion of ppoC inhibited G protein signaling pathways more, and the ΔppoC strain had more defective growth and development as well as reduced pathogenicity and patulin production (p < 0.05). Therefore, PePpoC proteins affect more growth and development, patulin biosynthesis and pathogenicity of P. expansum by regulating C8 MVOC-mediated G protein signaling transduction.
Effects of the Subscription-Based Partitioned Pricing Strategy of Digital Content Platforms on User Willingness to Purchase
Authors: Kang Jun , Su Caiyun , Lan Jingyi , Chen Libin
Published: November 2024

This research explores how the partitioned pricing strategy of premium subscriptions affects user willingness to purchase with two studies. Study 1 finds that all-inclusive pricing is more effective than traditional partitioned pricing (i.e., partitioned pricing in Single) and the two new formats of partitioned pricing (i.e., partitioned pricing in Combination and partitioned pricing in Blind Box) in terms of increasing user willingness to purchase due to the induced higher perceived value and perceived price fairness. Study 2 further finds that partitioned pricing in Combination or partitioned pricing in Blind Box (vs. partitioned pricing in Single) can affect perceived value positively via perceived playfulness and negatively via user-perceived price complexity simultaneously. These indirect effects hold across hedonic and utilitarian digital content products. These findings contribute to the literature on the pricing strategies of subscription-based digital content platforms. This research also provides suggestions for digital content platforms to adopt that will help them design their partitioned pricing strategies effectively.
Rhotic Variation in Brazilian Portuguese
Authors: Ramsammy Michael , Raposo de Medeiros Beatriz
Published: November 2024

We present acoustic and articulatory data from an experiment designed to test the phonetic variability of rhotics in Brazilian Portuguese, focusing on the São Paulo variety. Ultrasound tongue imaging was used to examine the realisation of rhotics in a range of phonological environments. Our analysis reveals that word-initial and intervocalic fricatives are acoustically and articulatorily distinct for most speakers. We attribute a tendency for utterance-initial fricatives to display longer duration, less voicing, and greater tongue-dorsum displacement than word-medial intervocalic counterparts to phonetic enhancement at the site of a major prosodic boundary. Similarly, rhotic taps in utterance-final position show a tendency for devoicing and frication (aspiration or assibilation) speaker-dependently. By comparison, word-medial pre-consonantal and intervocalic taps are characterised by shorter durations and greater voicing: hence, a pattern of phonetic reduction in prosodically weaker environments. We relate our findings to theoretical debates around the phonological status of rhotics in Portuguese. Whilst not providing conclusive proof in favour of any one particular approach, our results highlight the need to recognise the reality of prosodically driven strengthening in developing a full account of rhotic variation in the variety.
Research on the Measurement Technology for Pretension Stress on Small-Sized Bolts Based on the Piezoelectric Ultrasonic Resonance Method
Authors: Chen Bing , Luo Chunlang , Xia Li , Xu Lintao , Yan Guanglong , Qiu Feifei , Gou Guoqing
Published: November 2024

With the widespread application of small-sized bolts in aerospace and other fields, the demand for measuring their connection structures is increasing. Currently, although ultrasonic longitudinal wave methods are commonly used for bolt pretension stress measurement, their accuracy is limited for small-sized bolts. This paper proposes a piezoelectric acoustic resonance method (PZTAR) for small-sized bolt pretension stress measurement based on acoustic elasticity theory, ultrasonic resonance principles, and a bolt stress–strain model. The method involves analyzing the ultrasonic time-domain signals of small-sized bolts under load in the frequency domain to better evaluate the changes in the ultrasonic frequencies under different pretension stress. The effectiveness of this method is verified through pretension stress measurement experiments. The results indicate that the proposed ultrasonic resonance method achieves an average error of less than 5% for M5 specification bolts. Compared to traditional ultrasonic time delay methods, the proposed method demonstrates higher measurement accuracy. Additionally, the ultrasonic resonance method exhibits better robustness during the measurement process.
Strength Properties and Microscopic Experimental Study of Modified Sawdust Based on Solid Waste Synergistic Utilization
Authors: Cheng Yu , Jiang Na , Wang Wentong , Jin Lu
Published: November 2024

Sawdust is the cutting tailings produced during stone processing, which is difficult to deal with and has a huge stock. Therefore, it is particularly important to enhance the comprehensive utilization of sawdust. The aim of this study was to synergistically utilize sawdust with other industrial wastes (fly ash, silt, and red mud), add cement as a curing agent to prepare modified sawdust, and analyze its performance through an unconfined compressive strength test, dry and wet cycle tests, and SEM. The results showed that the compressive strength of modified sawdust with different solid waste dosages was more than 2.5 MPa after 7 days of maintenance, the strength was basically more than 4 MPa after 28 days of maintenance, and 8% solid waste dosage had the best effect. In addition, the modified saw mud with 8% fly-ash dosage had superior wet and dry cycle resistance, with expansion and shrinkage lower than 0.5% and good stability. This study provides a new idea for the synergistic utilization of saw mud and other solid wastes, and it is recommended to consider 8% solid waste dosage to optimize the performance in practical applications.
Thermal Properties of Polysiloxane/Ag Nanocomposites with Different Network Structures and Distributions of Si–H Groups
Authors: Wójcik-Bania Monika , Stochmal Edyta
Published: November 2024

Polysiloxanes with silver nanoparticles (Ag NPs) have garnered attention for their distinctive physicochemical properties, which make them promising candidates for advanced material applications. This study presents a systematic investigation into the thermal properties and degradation mechanisms of polysiloxane/Ag nanocomposites, emphasising the innovative incorporation of Ag NPs directly into polysiloxane networks via in situ reduction of Ag⁺ ions by Si-H groups. Six polysiloxane matrices were synthesised by hydrosilylation of poly(methylhydrosiloxane) (PMHS) or poly(vinylsiloxane) (polymer V3) with three cross-linking agents of varying molecular structures and functionality. Thermogravimetric analysis combined with mass spectrometry revealed that the introduction of Ag NPs alters the thermal properties of polysiloxane networks, primarily affecting the redistribution of Si bonds that occurs during the pyrolysis of these systems. Monitoring the pyrolysis process using FTIR spectroscopy allowed us to investigate the effect of the presence of Ag NPs on the degradation mechanism of the studied nanocomposites. The presence of the free-carbon phase and metallic silver phase in the Ag-containing silicon oxycarbide materials obtained was confirmed by Raman spectroscopy and XRD analyses, respectively. These findings demonstrate the possibility of fabricating Ag/SiOC materials with ceramic residues in the range of 43 to 84%. This work provides new insights into the thermal behaviour of polysiloxane/Ag nanocomposites and underscores their potential for high-performance applications in thermally demanding environments.
High Strain Rate Deformation of Heat-Treated AA2519 Alloy
Authors: Olasumboye Adewale , Omoniyi Peter , Owolabi Gbadebo
Published: November 2024

This study examined the effects of heat treatment on the microstructure and dynamic deformation characteristics of AA2519 aluminum alloy in T4, T6, and T8 tempers under high strain rates of 1000–4000 s−1. A Split Hopkinson pressure bar (SHPB) was utilized to characterize the mechanical response, and microstructural analysis was performed to examine the material’s microstructure. The findings indicated varied deformation across all three temper conditions. The dynamic behavior of each temper is influenced by its strength properties, which are determined by the aging type and the subsequent transformation of strengthening precipitates, along with the initial microstructure. At a strain rate of 1500 s−1, AA2519-T6 demonstrated a peak dynamic yield strength of 509 MPa and a flow stress of 667 MPa. These values are comparable to those recorded for AA2519-T8 at a strain rate of 3500 s−1. AA2519-T4 exhibited the lowest strength and flow stress characteristics. The T6 temper demonstrated initial stress collapse, dynamic strain aging, and an increased tendency for shear band formation and fracture within the defined strain rate range. The strain rates all showed similar trends in terms of strain hardening rate. The damage evolution of the alloy primarily involved the nucleation, shearing, and cracking of dispersoid particles.