bluuebunny/crossref_metadata_2025 huggingface dataset:
To Alleviate or Elevate the Euroamerican Genealogy Fever
Authors: Riddell Iain
Published: March 2018

There has been a quiet cultural drift towards professionalism in genealogy over the last two decades. Developments in the UK on this subject have resulted in educational offerings that support professionalism through accountability to service consumers while a US debate has pushed for a recognised and regarded scholarship to underpin the professional genealogist. This article places the educational and learning needs of three broad groups of genealogists into the framework of the professional debate in its generality and genealogical specifics. With a concentration on the British context, the article considers the cultural–commercial signals and support offered to ‘armchair enthusiasts’; the emerging models of professional education and formation aimed at lineage makers and the ongoing fractured models of scholastic genealogy. Looking ahead at educational needs, genealogy like other professions is now under threat from advances in artificial intelligence and algorithms, which could slice through the underpinnings of genealogical professionalism. The article concludes with a discussion of an alternative approach to genealogical education derived from the proposition that professionalism is to be found in the outputs and outcomes rather than the organisation of the practitioners of economic activity. From this stance, the needs of a full range of people pursuing genealogy can be addressed and their work informed by the developing understanding of Euroamerican kinship.
A Novel Hybrid Sequence-Based Model for Identifying Anticancer Peptides
Authors: Xu Lei , Liang Guangmin , Wang Longjie , Liao Changrui
Published: March 2018

Cancer is a serious health issue worldwide. Traditional treatment methods focus on killing cancer cells by using anticancer drugs or radiation therapy, but the cost of these methods is quite high, and in addition there are side effects. With the discovery of anticancer peptides, great progress has been made in cancer treatment. For the purpose of prompting the application of anticancer peptides in cancer treatment, it is necessary to use computational methods to identify anticancer peptides (ACPs). In this paper, we propose a sequence-based model for identifying ACPs (SAP). In our proposed SAP, the peptide is represented by 400D features or 400D features with g-gap dipeptide features, and then the unrelated features are pruned using the maximum relevance-maximum distance method. The experimental results demonstrate that our model performs better than some existing methods. Furthermore, our model has also been extended to other classifiers, and the performance is stable compared with some state-of-the-art works.
CD99: A Cell Surface Protein with an Oncojanus Role in Tumors
Authors: Manara Maria , Pasello Michela , Scotlandi Katia
Published: March 2018

The cell surface molecule CD99 has gained interest because of its involvement in regulating cell differentiation and adhesion/migration of immune and tumor cells. However, the molecule plays an intriguing and dual role in different cell types. In particular, it acts as a requirement for cell malignancy or as an oncosuppressor in tumors. In addition, the gene encodes for two different isoforms, which also act in opposition inside the same cell. This review highlights key studies focusing on the dual role of CD99 and its isoforms and discusses major critical issues, challenges, and strategies for overcoming those challenges. The review specifically underscores the properties that make the molecule an attractive therapeutic target and identifies new relationships and areas of study that may be exploited. The elucidation of the spatial and temporal control of the expression of CD99 in normal and tumor cells is required to obtain a full appreciation of this molecule and its signaling.
Environmental and Health: The Importance of Tremolite Occurence in the Pollino Geopark (Southern Italy)
Authors: Dichicco Maria , Laurita Salvatore , Sinisi Rosa , Battiloro Raimondo , Rizzo Giovanna
Published: March 2018

Worldwide studies have been done about the toxicity and carcinogenicity of asbestos minerals occurring in ophiolitic rocks. Inhalation of asbestos due to environmental exposure could cause malignant mesothelioma and lung cancers. In particular, the ophiolitic rocks in Tethyan realm represents a serious enivironmental concern due to both the presence of asbestos-like minerals and the large Cr abundance that is prone to solubilisation as CrVI. At the Pollino Geopark (southern Apennines, Italy), serpentinites-rich ophiolite rocks and sediments of the Frido Unit crop-out. In these rocks, tremolite, an asbestos-like mineral, is typically intergrown with fibrous antigorite and chrysotile. Tremolite shows acicular, friable, fibrous, and elongated habitus, can be easily released into the environment as a result of both natural processes and anthropogenic activities. In the analyzed rocks, tremolite is present mainly in veins as much as in the matrix and forms crowns around clinopyroxene porphyroclasts. The different analytical techniques showed the recognition of the amphibole-like minerals (actinolite and tremolite) that are the dominant phases, with a small percentage of Fe2+. The presence of Fe in the “ideal” tremolite asbestos could cause pathological effects for the human living in the Pollino Geopark. This study has several environmental relevant implications, including, for example, the realization of national health protecting programs and the mapping of natural sites characterized by the presence of asbestos minerals in Pollino Geopark and in others area where outcrop asbestos bearing ophiolitic rocks.
Prioritizing Type of Industry through Health Risk Assessment of Occupational Exposure to Dimethylformamide in the Workplace
Authors: Lee Junghyun , Hahm Miran , Huh Da-An , Byeon Sang-Hoon
Published: March 2018

The purpose of this study was to classify hazards at an industrial level and evaluate the exposure risks of workers exposed to dimethylformamide (DMF) used as a solvent in the workplace and to determine industries that need priority measures in managing DMF exposure. We calculated hazard quotients at an industrial level. The exposure data of DMF in the workplace were obtained from the work environment monitoring program provided by the Korea Occupational Safety and Health Agency. The evaluation was conducted on textile manufacturing, leather manufacturing, chemical manufacturing, pharmaceutical manufacturing, and rubber manufacturing industries, which have many unit work sites handling DMF. The highest central tendency exposure and reasonable maximum exposure were 2.13 and 18.66 mg/m3 for the rubber product manufacturing industry, respectively. A total of 63.8% of workplaces in the textile manufacturing sector had a hazard quotient higher than 1. The highest risk for exposure to DMF is in the rubber and plastic manufacturing industry, and the lowest risk was in the medical materials and pharmaceutical manufacturing sector. Based on this study, effective management of DMF exposure could be achieved by establishing priority management measures for the textile and rubber and plastic product industries.
Internalized Transphobia, Resilience, and Mental Health: Applying the Psychological Mediation Framework to Italian Transgender Individuals
Authors: Scandurra Cristiano , Bochicchio Vincenzo , Amodeo Anna , Esposito Concetta , Valerio Paolo , Maldonato Nelson , Bacchini Dario , Vitelli Roberto
Published: March 2018

Transgender and gender nonconforming (TGNC) people are a highly-stigmatized population. For this reason, they might internalize society’s normative gender attitudes and develop negative mental health outcomes. As an extension of the minority stress model, the psychological mediation framework sheds light on psychological processes through which anti-transgender discrimination might affect mental health. Within this framework, the current study aimed at assessing in 149 TGNC Italian individuals the role of internalized transphobia as a mediator between anti-transgender discrimination and mental health, considering resilience as the individual-level coping mechanism buffering this relationship. The results suggest that both indicators of internalized transphobia (i.e., shame and alienation) mediate the relationship between anti-transgender discrimination and depression, while only alienation mediates the relationship between anti-transgender discrimination and anxiety. Furthermore, the results suggest that the indirect relation between anti-transgender discrimination and anxiety through alienation is conditional on low and moderate levels of resilience. Findings have important implications for clinical practice and psycho-social interventions to reduce stigma and stress caused by interpersonal and individual stigma.
Representing Time-Dynamic Geospatial Objects on Virtual Globes Using CZML—Part I: Overview and Key Issues
Authors: Zhu Liangfeng , Wang Zhongliang , Li Zhiwen
Published: March 2018

Cesium Markup Language (CZML) is an emerging specification for the representation and exchange of time-dynamic geospatial objects on virtual globes. The principal focus of CZML is on the definition of time-varying characteristics that are important for applications of geospatial objects, such as changeable positions/extents, graphical appearances, and other geospatial properties. Due to its unique ability to stream massive geospatial datasets, CZML is ideally suited for efficient, incremental streaming to the client in the network environment. Our goal is to explore and outline the overall perspective of CZML as an efficient schema for representing time-dynamic geospatial objects on virtual globes. Such a perspective is the topic of the two present companion papers. Here, in the first part, we provide an overview of CZML and explore two key issues, and their associated solutions, for representing time-dynamic geospatial objects using CZML: one is how to use CZML properties to describe time-varying characteristics of geospatial objects, and the other is how to use CZML to support streaming data. These innovative improvements provide highly-efficient and more reliable supports for representing time-dynamic geospatial objects. The relevant applications, academic influence, and future developments of CZML are explored in a second paper.
Fraxinellone Attenuates Rheumatoid Inflammation in Mice
Authors: Jung Seung , Lee Jaeseon , Baek Seung , Lee Juhyun , Jang Se , Hong Seung-Min , Park Jin-Sil , Cho Mi-La , Park Sung-Hwan , Kwok Seung-Ki
Published: March 2018

This study aimed to evaluate the therapeutic effect of fraxinellone on inflammatory arthritis and identify the underlying mechanisms. Fraxinellone (7.5 mg/kg) or a vehicle control was injected into mice with collagen-induced arthritis (CIA). The severity of arthritis was evaluated clinically and histologically. The differentiation of CD4+ T cells and CD19+ B cells was investigated in the presence of fraxinellone. Osteoclastogenesis after fraxinellone treatment was evaluated by staining with tartrate-resistant acid phosphatase (TRAP) and by measuring the mRNA levels of osteoclastogenesis-related genes. Fraxinellone attenuated the clinical and histologic features of inflammatory arthritis in CIA mice. Fraxinellone suppressed the production of interleukin-17 and the expression of RAR-related orphan receptor γ t and phospho-signal transducer and activator of transcription 3 in CD4+ T cells. CD19+ B cells showed lower expression of activation-induced cytidine deaminase and B lymphocyte-induced maturation protein-1 after treatment with fraxinellone. The formation of TRAP-positive cells and the expression of osteoclastogenesis-related markers were reduced in the presence of fraxinellone. Inhibition of interleukin-17 and osteoclastogenesis was also observed in experiments using human peripheral mononuclear cells. Fraxinellone alleviated synovial inflammation and osteoclastogenesis in mice. The therapeutic effect of fraxinellone was associated with the inhibition of cellular differentiation and activation. The data suggests that fraxinellone could be a novel treatment for inflammatory arthritis, including rheumatoid arthritis.
Immunology Guides Skeletal Muscle Regeneration
Authors: Sass F. , Fuchs Michael , Pumberger Matthias , Geissler Sven , Duda Georg , Perka Carsten , Schmidt-Bleek Katharina
Published: March 2018

Soft tissue trauma of skeletal muscle is one of the most common side effects in surgery. Muscle injuries are not only caused by accident-related injuries but can also be of an iatrogenic nature as they occur during surgical interventions when the anatomical region of interest is exposed. If the extent of trauma surpasses the intrinsic regenerative capacities, signs of fatty degeneration and formation of fibrotic scar tissue can occur, and, consequentially, muscle function deteriorates or is diminished. Despite research efforts to investigate the physiological healing cascade following trauma, our understanding of the early onset of healing and how it potentially determines success or failure is still only fragmentary. This review focuses on the initial physiological pathways following skeletal muscle trauma in comparison to bone and tendon trauma and what conclusions can be drawn from new scientific insights for the development of novel therapeutic strategies. Strategies to support regeneration of muscle tissue after injury are scarce, even though muscle trauma has a high incidence. Based on tissue specific differences, possible clinical treatment options such as local immune-modulatory and cell therapeutic approaches are suggested that aim to support the endogenous regenerative potential of injured muscle tissues.
Molecular Characterization and Overexpression of VpRPW8s from Vitis pseudoreticulata Enhances Resistance to Phytophthora capsici in Nicotiana benthamiana
Authors: Lai Gongti , Fu Peining , Liu Yunxiao , Xiang Jiang , Lu Jiang
Published: March 2018

RPW8 genes are atypical broad-spectrum genes that provide resistance to powdery mildew, downy mildew, the cauliflower mosaic virus in Arabidopsis thaliana, and powdery mildew in tobacco. They play important roles in basal plant pathogen defense. They also provide insights into a novel disease resistance mechanism. In this study, we report on homologous RPW8 genes in Vitis pseudoreticulata. Five VpRPW8 genes were cloned; their Open Reading Frame (ORF) sequences ranged from 1994 base pairs to 2478 base pairs. They were comprised of five exons and four introns and shared 78.66% identity. Their proteins had typical conserved RPW8 and NB-LRR (the nucleotide-binding site and the leucine-rich repeats) domains (except VpRPW8-d, which lacked LRR domains). Prokaryotic expression results were consistent with predicted molecular weights. All five RPW8 genes were located in the cytoplasm. Quantitative real-time PCR (qRT-PCR) analysis showed that VpRPW8s in V. pseudoreticulata were induced by Plasmopara viticola, but nearly only VvRPW8-d genes were induced in Vitis vinifera. Furthermore, a VpRPW8 transgenic tobacco system was established. Overexpressed VpRPW8s enhanced resistance to Phytophthora capsici and VpRPW8s conferred varying degrees of resistance to Ph. capsici in Nicotiana benthamiana. Our study presents novel members of the plant RPW8 family and suggests that VpRPW8s are involved in enhanced resistance to P. viticola and Ph. capsici.
Structure/Function Analysis of Cotton-Based Peptide-Cellulose Conjugates: Spatiotemporal/Kinetic Assessment of Protease Aerogels Compared to Nanocrystalline and Paper Cellulose
Authors: Edwards J. , Fontenot Krystal , Liebner Falk , Pircher Nicole , French Alfred , Condon Brian
Published: March 2018

Nanocellulose has high specific surface area, hydration properties, and ease of derivatization to prepare protease sensors. A Human Neutrophil Elastase sensor designed with a nanocellulose aerogel transducer surface derived from cotton is compared with cotton filter paper, and nanocrystalline cellulose versions of the sensor. X-ray crystallography was employed along with Michaelis–Menten enzyme kinetics, and circular dichroism to contrast the structure/function relations of the peptide-cellulose conjugate conformation to enzyme/substrate binding and turnover rates. The nanocellulosic aerogel was found to have a cellulose II structure. The spatiotemporal relation of crystallite surface to peptide-cellulose conformation is discussed in light of observed enzyme kinetics. A higher substrate binding affinity (Km) of elastase was observed with the nanocellulose aerogel and nanocrystalline peptide-cellulose conjugates than with the solution-based elastase substrate. An increased Km observed for the nanocellulosic aerogel sensor yields a higher enzyme efficiency (kcat/Km), attributable to binding of the serine protease to the negatively charged cellulose surface. The effect of crystallite size and β-turn peptide conformation are related to the peptide-cellulose kinetics. Models demonstrating the orientation of cellulose to peptide O6-hydroxymethyl rotamers of the conjugates at the surface of the cellulose crystal suggest the relative accessibility of the peptide-cellulose conjugates for enzyme active site binding.
Distributed State Estimation under State Inequality Constraints with Random Communication over Multi-Agent Networks
Authors: Hu Chen , Li Zhenhua , Lin Haoshen , He Bing , Liu Gang
Published: March 2018

In this paper, we investigate distributed state estimation for multi-agent networks with random communication, where the state is constrained by an inequality. In order to deal with the problem of environmental/communication uncertainties and to save energy, we introduce two random schemes, including a random sleep scheme and an event-triggered scheme. With the help of Kalman-consensus filter and projection on the constrained set, we propose two random distributed estimation algorithm. The estimation of each agent is achieved by projecting the consensus estimate, which is obtained by virtue of random exchange information with its neighbors. The estimation error is shown to be bounded with probability one when the agents randomly take the measurement or communicate with their neighbors. We show the stability of proposed algorithm based on Lyapunov method and projection and demonstrate their effectiveness via numerical simulations.
Atomic Layer Deposition on Porous Materials: Problems with Conventional Approaches to Catalyst and Fuel Cell Electrode Preparation
Authors: Onn Tzia , Küngas Rainer , Fornasiero Paolo , Huang Kevin , Gorte Raymond
Published: March 2018

Atomic layer deposition (ALD) offers exciting possibilities for controlling the structure and composition of surfaces on the atomic scale in heterogeneous catalysts and solid oxide fuel cell (SOFC) electrodes. However, while ALD procedures and equipment are well developed for applications involving flat surfaces, the conditions required for ALD in porous materials with a large surface area need to be very different. The materials (e.g., rare earths and other functional oxides) that are of interest for catalytic applications will also be different. For flat surfaces, rapid cycling, enabled by high carrier-gas flow rates, is necessary in order to rapidly grow thicker films. By contrast, ALD films in porous materials rarely need to be more than 1 nm thick. The elimination of diffusion gradients, efficient use of precursors, and ligand removal with less reactive precursors are the major factors that need to be controlled. In this review, criteria will be outlined for the successful use of ALD in porous materials. Examples of opportunities for using ALD to modify heterogeneous catalysts and SOFC electrodes will be given.
Honeybees Tolerate Cyanogenic Glucosides from Clover Nectar and Flowers
Authors: Lecocq Antoine , Green Amelia , Pinheiro De Castro Érika , Olsen Carl , Jensen Annette , Zagrobelny Mika
Published: March 2018

Honeybees (Apis mellifera) pollinate flowers and collect nectar from many important crops. White clover (Trifolium repens) is widely grown as a temperate forage crop, and requires honeybee pollination for seed set. In this study, using a quantitative LC-MS (Liquid Chromatography-Mass Spectrometry) assay, we show that the cyanogenic glucosides linamarin and lotaustralin are present in the leaves, sepals, petals, anthers, and nectar of T. repens. Cyanogenic glucosides are generally thought to be defense compounds, releasing toxic hydrogen cyanide upon degradation. However, increasing evidence indicates that plant secondary metabolites found in nectar may protect pollinators from disease or predators. In a laboratory survival study with chronic feeding of secondary metabolites, we show that honeybees can ingest the cyanogenic glucosides linamarin and amygdalin at naturally occurring concentrations with no ill effects, even though they have enzyme activity towards degradation of cyanogenic glucosides. This suggests that honeybees can ingest and tolerate cyanogenic glucosides from flower nectar. Honeybees retain only a portion of ingested cyanogenic glucosides. Whether they detoxify the rest using rhodanese or deposit them in the hive should be the focus of further research.
Long-Term Increase of Radiographic Damage and Disability in Patients with RA in Relation to Disease Duration in the Era of Biologics. Results from the SCQM Cohort
Authors: Heinimann Katja , Von Kempis Johannes , Sauter Rafael , Schiff Michael , Sokka-Isler Tuulikki , Schulze-Koops Hendrik , Müller Rüdiger
Published: March 2018

Objectives: There is little information on the relation between disease duration, disability and radiographic outcome since the introduction of biologics into the therapy of rheumatoid arthritis (RA). No long -term cohort studies have been conducted on this subject so far. To analyse radiographic damage, disability, and disease activity in RA-patients dependent on disease duration in the Swiss national RA cohort (SCQM). Methods: The primary outcome was the association between the radiographic destruction, assessed by Ratingen scores, and disease duration. All patients with at least one clinical visit were analysed with polynomial and multiple negative binomial models. Results: The disease duration in the 8678 patients with available radiographs analysed ranged between less than 1 and more than 65 years (median 8.3). Disease duration and radiographic destruction were significantly associated with an average increase of Ratingen scores by 8.3% per year. Apart from disease duration, positive rheumatoid factor was the strongest predictor for radiographic destruction. While DAS28-scores remained stable in patients with a disease duration of more than 5 years (median DAS28 2.8), HAQ-DI scores increased continuously by 0.018 for each additional year. Conclusion: In this RA cohort, patients show a continuous increase of articular destruction and physical disability in parallel with disease duration. Even when nowadays a satisfactory control of disease activity can be achieved in most patients, RA remains a destructive disease leading to joint destruction and physical disability in many patients.
Thermal Properties of Zeolite-Containing Composites
Authors: Shimonosono Taro , Hirata Yoshihiro , Nishikawa Kyohei , Sameshima Soichiro , Sodeyama Kenichi , Masunaga Takuro , Yoshimura Yukio
Published: March 2018

A zeolite (mordenite)–pore–phenol resin composite and a zeolite–pore–shirasu glass composite were fabricated by hot-pressing. Their thermal conductivities were measured by a laser flash method to determine the thermal conductivity of the monolithic zeolite with the proposed mixing rule. The analysis using composites is useful for a zeolite powder with no sinterability to clarify its thermal properties. At a low porosity <20%, the thermal conductivity of the composite was in excellent agreement with the calculated value for the structure with phenol resin or shirasu glass continuous phase. At a higher porosity above 40%, the measured value approached the calculated value for the structure with pore continuous phase. The thermal conductivity of the monolithic mordenite was evaluated to be 3.63 W/mK and 1.70–2.07 W/mK at room temperature for the zeolite–pore–phenol resin composite and the zeolite–pore–shirasu glass composite, respectively. The analyzed thermal conductivities of monolithic mordenite showed a minimum value of 1.23 W/mK at 400 °C and increased to 2.51 W/mK at 800 °C.
Cure Cycle Optimization of Rapidly Cured Out-Of-Autoclave Composites
Authors: Dong Anqi , Zhao Yan , Zhao Xinqing , Yu Qiyong
Published: March 2018

Out-of-autoclave prepreg typically needs a long cure cycle to guarantee good properties as the result of low processing pressure applied. It is essential to reduce the manufacturing time, achieve real cost reduction, and take full advantage of out-of-autoclave process. The focus of this paper is to reduce the cure cycle time and production cost while maintaining high laminate quality. A rapidly cured out-of-autoclave resin and relative prepreg were independently developed. To determine a suitable rapid cure procedure for the developed prepreg, the effect of heating rate, initial cure temperature, dwelling time, and post-cure time on the final laminate quality were evaluated and the factors were then optimized. As a result, a rapid cure procedure was determined. The results showed that the resin infiltration could be completed at the end of the initial cure stage and no obvious void could be seen in the laminate at this time. The laminate could achieve good internal quality using the optimized cure procedure. The mechanical test results showed that the laminates had a fiber volume fraction of 59–60% with a final glass transition temperature of 205 °C and excellent mechanical strength especially the flexural properties.
Microporosity and CO2 Capture Properties of Amorphous Silicon Oxynitride Derived from Novel Polyalkoxysilsesquiazanes
Authors: Iwase Yoshiaki , Horie Yoji , Honda Sawao , Daiko Yusuke , Iwamoto Yuji
Published: March 2018

Polyalkoxysilsesquiazanes ([ROSi(NH)1.5]n, ROSZ, R = Et, nPr, iPr, nBu, sBu, nHex, sHex, cHex, decahydronaphthyl (DHNp)) were synthesized by ammonolysis at −78 °C of alkoxytrichlorosilane (ROSiCl3), which was isolated by distillation as a reaction product of SiCl4 and ROH. The simultaneous thermogravimetric and mass spectrometry analyses of the ROSZs under helium revealed a common decomposition reaction, the cleavage of the oxygen–carbon bond of the RO group to evolve alkene as a main gaseous species formed in-situ, leading to the formation of microporous amorphous Si–O–N at 550 °C to 800 °C. The microporosity in terms of the peak of the pore size distribution curve located within the micropore size range (<2 nm) and the total micropore volume, as well as the specific surface area (SSA) of the Si–O–N, increased consistently with the molecular size estimated for the alkene formed in-situ during the pyrolysis. The CO2 capture capacity at 0 °C of the Si–O–N material increased consistently with its SSA, and an excellent CO2 capture capacity of 3.9 mmol·g−1 at 0 °C and CO2 1 atm was achieved for the Si–O–N derived from DHNpOSZ having an SSA of 750 m2·g−1. The CO2 capture properties were further discussed based on their temperature dependency, and a surface functional group of the Si–O–N formed in-situ during the polymer/ceramics thermal conversion.
Biphasic Scaffolds from Marine Collagens for Regeneration of Osteochondral Defects
Authors: Bernhardt Anne , Paul Birgit , Gelinsky Michael
Published: March 2018

Background: Collagens of marine origin are applied increasingly as alternatives to mammalian collagens in tissue engineering. The aim of the present study was to develop a biphasic scaffold from exclusively marine collagens supporting both osteogenic and chondrogenic differentiation and to find a suitable setup for in vitro chondrogenic and osteogenic differentiation of human mesenchymal stroma cells (hMSC). Methods: Biphasic scaffolds from biomimetically mineralized salmon collagen and fibrillized jellyfish collagen were fabricated by joint freeze-drying and crosslinking. Different experiments were performed to analyze the influence of cell density and TGF-β on osteogenic differentiation of the cells in the scaffolds. Gene expression analysis and analysis of cartilage extracellular matrix components were performed and activity of alkaline phosphatase was determined. Furthermore, histological sections of differentiated cells in the biphasic scaffolds were analyzed. Results: Stable biphasic scaffolds from two different marine collagens were prepared. An in vitro setup for osteochondral differentiation was developed involving (1) different seeding densities in the phases; (2) additional application of alginate hydrogel in the chondral part; (3) pre-differentiation and sequential seeding of the scaffolds and (4) osteochondral medium. Spatially separated osteogenic and chondrogenic differentiation of hMSC was achieved in this setup, while osteochondral medium in combination with the biphasic scaffolds alone was not sufficient to reach this ambition. Conclusions: Biphasic, but monolithic scaffolds from exclusively marine collagens are suitable for the development of osteochondral constructs.
Cytotoxic Polyhydroxysteroidal Glycosides from Starfish Culcita novaeguineae
Authors: Lu Yunyang , Li Hu , Wang Minchang , Liu Yang , Feng Yingda , Liu Ke , Tang Haifeng
Published: March 2018

Four new polyhydroxysteroidal glycosides—culcinosides A–D (1, 2, 4, and 7)—along with three known compounds—echinasteroside C (3), linckoside F (5), and linckoside L3 (6)—were isolated from the ethanol extract of starfish Culcita novaeguineae collected from the Xisha Islands of the South China Sea. The structures of new compounds were elucidated through extensive spectroscopic studies and chemical evidence, especially two-dimensional (2D) NMR techniques. The cytotoxicity of the new compounds against human glioblastoma cell lines U87, U251, and SHG44 were evaluated.
The Association between Body Mass Index and Health-Related Quality of Life in Treatment-Seeking Arab Adults with Obesity
Authors: Itani Leila , Calugi Simona , Dalle Grave Riccardo , Kreidieh Dima , El Kassas Germine , El Masri Dana , Tannir Hana , Harfoush Aya , El Ghoch Marwan
Published: March 2018

Few studies have thus far been carried out on Health-Related Quality of Life (HRQoL) and obesity in Arab-speaking countries, an issue that we therefore set out to investigate in this study. HRQoL was assessed by the validated Arabic version of the ORWELL 97 questionnaire in 129 treatment-seeking individuals with obesity referred to the Nutritional and Weight Management Outpatient Clinic at the Department of Nutrition and Dietetics of Beirut Arab University (BAU) in Lebanon, and 129 normal-weight participants of similar age and gender. Participants with obesity, regardless of gender, displayed higher total ORWELL 97 scores when compared with normal-weight controls, indicating that obesity is associated with lower HRQoL. Linear regression analysis showed that a higher body mass index (BMI) is associated with an increase in ORWELL 97 scores, but only among female, not male, participants with obesity (β = 2.89, 95% confidence interval (CI) = 1.43–4.53, p < 0.001). Moreover, logistic regression analysis showed that a one unit increase in BMI increases the odds of an ORWELL 97 score ≥ 71.75—considered indicative of a clinically significant impairment of HRQoL—by nearly 23% (odds ratio (OR), 95% CI = 1.23, 1.09–1.40, p < 0.05). If confirmed, our findings should prompt clinicians operating in Arab countries to encourage patients with obesity to initiate and persevere in weight-loss programs at the earliest opportunity.
A Correlation between the Ultimate Shear Stress and the Thickness Affected by Intermetallic Compounds in Friction Stir Welding of Dissimilar Aluminum Alloy–Stainless Steel Joints
Authors: Picot Florent , Gueydan Antoine , Martinez Mayerling , Moisy Florent , Hug Eric
Published: March 2018

In this work, Friction Stir Welding (FSW) was applied to join a stainless steel 316L and an aluminum alloy 5083. Ranges of rotation and translation speeds of the tool were used to obtain welding samples with different heat input coefficients. Depending on the process parameters, the heat generated by FSW creates thin layers of Al-rich InterMetallic Compound (IMC) mainly composed of FeAl3, identified by energy dispersive spectrometry. Traces of Fe2Al5 were also depicted in some samples by X-ray diffraction analysis and transmission electron microscopy. Monotonous tensile tests performed on the weld joint show the existence of a maximum mechanical resistance for a judicious choice of rotation and translation speeds. It can be linked to an affected zone of average thickness of 15 µm which encompass the presence of IMC and the chaotic mixing caused by plastic deformation in this area. A thickness of less than 15 µm is not sufficient to ensure a good mechanical resistance of the joint. For a thickness higher than 15 µm, IMC layers become more brittle and less adhesive due to high residual stresses which induces numerous cracks after cooling. This leads to a progressive decrease of the ultimate shear stress supported by the bond.
An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii
Authors: Aspatwar Ashok , Haapanen Susanna , Parkkila Seppo
Published: March 2018

Carbonic anhydrases (CAs) are metalloenzymes that are omnipresent in nature. CAs catalyze the basic reaction of the reversible hydration of CO2 to HCO3− and H+ in all living organisms. Photosynthetic organisms contain six evolutionarily different classes of CAs, which are namely: α-CAs, β-CAs, γ-CAs, δ-CAs, ζ-CAs, and θ-CAs. Many of the photosynthetic organisms contain multiple isoforms of each CA family. The model alga Chlamydomonas reinhardtii contains 15 CAs belonging to three different CA gene families. Of these 15 CAs, three belong to the α-CA gene family; nine belong to the β-CA gene family; and three belong to the γ-CA gene family. The multiple copies of the CAs in each gene family may be due to gene duplications within the particular CA gene family. The CAs of Chlamydomonas reinhardtii are localized in different subcellular compartments of this unicellular alga. The presence of a large number of CAs and their diverse subcellular localization within a single cell suggests the importance of these enzymes in the metabolic and biochemical roles they perform in this unicellular alga. In the present review, we update the information on the molecular biology of all 15 CAs and their metabolic and biochemical roles in Chlamydomonas reinhardtii. We also present a hypothetical model showing the known functions of CAs and predicting the functions of CAs for which precise metabolic roles are yet to be discovered.
Antibody-Based Agents in the Management of Antibiotic-Resistant Staphylococcus aureus Diseases
Authors: Speziale Pietro , Rindi Simonetta , Pietrocola Giampiero
Published: March 2018

Staphylococcus aureus is a human pathogen that can cause a wide spectrum of diseases, including sepsis, pneumonia, arthritis, and endocarditis. Ineffective treatment of a number of staphylococcal infections with antibiotics is due to the development and spread of antibiotic-resistant strains following decades of antibiotic usage. This has generated renewed interest within the scientific community in alternative therapeutic agents, such as anti-S. aureus antibodies. Although the role of antibodies in the management of S. aureus diseases is controversial, the success of this pathogen in neutralizing humoral immunity clearly indicates that antibodies offer the host extensive protection. In this review, we report an update on efforts to develop antibody-based agents, particularly monoclonal antibodies, and their therapeutic potential in the passive immunization approach to the treatment and prevention of S. aureus infections.
Sustained Cytotoxicity of Wogonin on Breast Cancer Cells by Encapsulation in Solid Lipid Nanoparticles
Authors: Baek Jong-Suep , Na Young-Guk , Cho Cheong-Weon
Published: March 2018

While wogonin has been known to have cytotoxicity against various cancer cells, its bioavailability and cytotoxicity are low due to its low water solubility. Therefore, wogonin-loaded solid lipid nanoparticles were fabricated using a hot-melted evaporation technique. The highest solubility of wogonin was observed in stearic acid. Hence, wogonin-loaded solid lipid nanoparticles were composed of stearic acid as the lipid matrix. The physicochemical properties of the wogonin-loaded solid lipid nanoparticles were evaluated by dynamic laser scattering and scanning electron microscopy. The wogonin-loaded solid lipid nanoparticles exhibited sustained and controlled release up to 72 h. In addition, it was observed that the wogonin-loaded solid lipid nanoparticles exhibited enhanced cytotoxicity and inhibited poly (ADP-ribose) polymerase in MCF-7 breast cancer cells. Overall, the results indicate that wogonin-loaded solid lipid nanoparticles could be an efficient delivery system for the treatment of breast cancer.
Randomised Controlled Trial Comparing Daily Versus Depot Vitamin D3 Therapy in 0–16-Year-Old Newly Settled Refugees in Western Australia Over a Period of 40 Weeks
Authors: Wadia Ushma , Soon Wayne , Chivers Paola , Thambiran Aesen , Burgner David , Cherian Sarah , Siafarikas Aris
Published: March 2018

Vitamin D deficiency is highly prevalent in newly settled refugees in Western Australia (WA). If adherence to daily vitamin D therapy is problematic, depot therapy is a therapeutic alternative. The aim of this study was to compare daily versus depot treatment and factors influencing the therapeutic outcome. Newly settled refugees (n = 151) with 25(OH)D levels less than 78 nmol/L were randomised to receive daily or depot vitamin D therapy with eight weekly interval follow up to 40 weeks. Biochemical and clinical parameters were collected at each visit. Generalized Linear Mixed Models (GLMM) examined the longitudinal changes over time controlling for confounders including age, gender, treatment arm, season, country of refuge/origin and sun exposure score. Participants were aged 5.5 months to 16.0 years (75 males, 83 females). Both treatment groups achieved vitamin D sufficiency. The daily treatment group had significantly higher 25(OH)D levels at each visit post baseline and a higher proportion of participants with levels above 50 nmol/L at all time points. Time, treatment group, calcium and sun exposure score were significant predictors of 25(OH)D serum levels. Depot vitamin D therapy is an alternative to daily treatment in this at-risk group of children and adolescents in whom treatment adherence is problematic.
Morphology, Mechanical Properties and Dimensional Stability of Biomass Particles/High Density Polyethylene Composites: Effect of Species and Composition
Authors: Mu Binshan , Wang Haigang , Hao Xiaolong , Wang Qingwen
Published: March 2018

The utilization of four types of biomass particles, including hardwood (poplar), softwood (radiata pine), crop (wheat straw) and bamboo (moso bamboo), as reinforcing fillers in preparing high density polyethylene (HDPE) based composites was studied. To improve interfacial compatibility, maleic anhydride grafted polyethylene (MAPE) was applied as the coupling agent. The effects of the biomass species on the mechanical and water absorption properties of the resulting composites were evaluated based on chemical composition analysis. A creep-recovery test was conducted in single cantilever mode using a dynamic mechanical analyzer. Results show that the four types of biomass particles had similar chemical compositions but different composition contents. Poplar particles with high cellulose content loading in the HDPE matrix exhibited higher tensile and flexure properties and creep resistance. Fracture morphology analysis indicated a weak particle-matrix interface in wheat straw based composites. Given the high crystallinity and minimum hemicellulose content, the moso bamboo reinforced composite showed high impact strength and better water resistance.
Synthesis, Characterization and Drug Loading of Multiresponsive p[NIPAm-co-PEGMA] (core)/p[NIPAm-co-AAc] (Shell) Nanogels with Monodisperse Size Distributions
Authors: Raju Rajesh , Bandyopadhyay Sulalit , Sharma Anuvansh , Gonzalez Susana , Carlsen Per , Gautun Odd , Glomm Wilhelm
Published: March 2018

We report the synthesis and properties of temperature- and pH-responsive p([NIPAm-co-PEGMA] (core)/[NIPAm-co-AAc] (shell)) nanogels with narrow size distributions, tunable sizes and increased drug loading efficiencies. The core-shell nanogels were synthesized using an optimized two-stage seeded polymerization methodology. The core-shell nanogels show a narrow size distribution and controllable physico-chemical properties. The hydrodynamic sizes, charge distributions, temperature-induced volume phase transition behaviors, pH-responsive behaviors and drug loading capabilities of the core-shell nanogels were investigated using transmission electron microscopy, zeta potential measurements, dynamic light scattering and UV-Vis spectroscopy. The size of the core-shell nanogels was controlled by polymerizing NIPAm with crosslinker poly(ethylene glycol) dimethacrylate (PEGDMA) of different molecular weights (Mn-200, 400, 550 and 750 g/mol) during the core synthesis. It was found that the swelling/deswelling kinetics of the nanogels was sharp and reversible; with its volume phase transition temperature in the range of 40–42 °C. Furthermore, the nanogels loaded with l-3,4-dihydroxyphenylalanine (L-DOPA), using a modified breathing-in mechanism, showed high loading and encapsulation efficiencies, providing potential possibilities of such nanogels for biomedical applications.
Land Consolidation Associations and the Management of Territories in Harsh Italian Environments: A Review
Authors: Beltramo Riccardo , Rostagno Andrea , Bonadonna Alessandro
Published: March 2018

Land fragmentation is a phenomenon that reduces the mechanical management of agricultural and grazing lands and, consequently, leads to the abandonment of agricultural practices in harsh environments. It puts the agricultural and/or agro-pastoral businesses in a difficult situation as they have small surfaces to manage that do not allow for sufficient profit. Some worldwide land consolidation initiatives have been set up to reduce this phenomenon, such as land funds. In this context, this paper is dedicated to an Italian approach, which can be carried over to other realities, aimed at safeguarding and managing territories in harsh environments. This approach, known as the “Associazione Fondiaria (ASFO)”, which can be roughly translated as “land consolidation association”, consolidates small portions of abandoned land in a functional manner. Indeed, a land consolidation association not only stimulates new entrepreneurial agricultural activities, but is also able to create employment in depressed areas. A SWOT (Strengths, Weaknesses, Opportunities, Threats) analysis was used to evaluate ASFOs, and their peculiarities were discussed.
Climate and Spring Phenology Effects on Autumn Phenology in the Greater Khingan Mountains, Northeastern China
Authors: Fu Yuanyuan , He Hong , Zhao Jianjun , Larsen David , Zhang Hongyan , Sunde Michael , Duan Shengwu
Published: March 2018

Vegetation phenology plays a key role in terrestrial ecosystem nutrient and carbon cycles and is sensitive to global climate change. Compared with spring phenology, which has been well studied, autumn phenology is still poorly understood. In this study, we estimated the date of the end of the growing season (EOS) across the Greater Khingan Mountains, China, from 1982 to 2015 based on the Global Inventory Modeling and Mapping Studies (GIMMS) normalized difference vegetation index third-generation (NDVI3g) dataset. The temporal correlations between EOS and climatic factors (e.g., preseason temperature, preseason precipitation), as well as the correlation between autumn and spring phenology, were investigated using partial correlation analysis. Results showed that more than 94% of the pixels in the Greater Khingan Mountains exhibited a delayed EOS trend, with an average rate of 0.23 days/y. Increased preseason temperature resulted in earlier EOS in most of our study area, except for the semi-arid grassland region in the south, where preseason warming generally delayed EOS. Similarly, EOS in most of the mountain deciduous coniferous forest, forest grassland, and mountain grassland forest regions was earlier associated with increased preseason precipitation, but for the semi-arid grassland region, increased precipitation during the preseason mainly led to delayed EOS. However, the effect of preseason precipitation on EOS in most of the Greater Khingan Mountains was stronger than that of preseason temperature. In addition to the climatic effects on EOS, we also found an influence of spring phenology on EOS. An earlier SOS led to a delayed EOS in most of the study area, while in the southern of mountain deciduous coniferous forest region and northern of semi-arid grassland region, an earlier SOS was often followed by an earlier EOS. These findings suggest that both climatic factors and spring phenology should be incorporated into autumn phenology models in order to improve prediction accuracy under present and future climate change scenarios.
The Development of a Low-Cost, Near Infrared, High-Temperature Thermal Imaging System and Its Application to the Retrieval of Accurate Lava Lake Temperatures at Masaya Volcano, Nicaragua
Authors: Wilkes Thomas , Stanger Leigh , Willmott Jon , Pering Tom , McGonigle Andrew , England Rebecca
Published: March 2018

Near infrared thermal cameras can provide useful low-cost imaging systems for high temperature applications, as an alternative to ubiquitous mid-/long-wavelength infrared systems. Here, we present a new Raspberry Pi-based near infrared thermal camera for use at temperatures of ≈>500 °C. We discuss in detail the building of the optical system, calibration using a Sakuma-Hattori model and quantification of uncertainties in remote temperature retrievals. We then present results from the deployment of the system on Masaya Volcano, Nicaragua, where the active lava lake was imaged. Temperatures reached a maximum of 1104 ± 14 °C and the lake radiative power output was found to range between 30 and 45 MW. To the best of our knowledge, this is the first published ground-based data on the thermal characteristics of this relatively nascent lava lake, which became visible in late 2015.
Object-Based Features for House Detection from RGB High-Resolution Images
Authors: Chen Renxi , Li Xinhui , Li Jonathan
Published: March 2018

Automatic building extraction from satellite images, an open research topic in remote sensing, continues to represent a challenge and has received substantial attention for decades. This paper presents an object-based and machine learning-based approach for automatic house detection from RGB high-resolution images. The images are first segmented by an algorithm combing a thresholding watershed transformation and hierarchical merging, and then shadows and vegetation are eliminated from the initial segmented regions to generate building candidates. Subsequently, the candidate regions are subjected to feature extraction to generate training data. In order to capture the characteristics of house regions well, we propose two kinds of new features, namely edge regularity indices (ERI) and shadow line indices (SLI). Finally, three classifiers, namely AdaBoost, random forests, and Support Vector Machine (SVM), are employed to identify houses from test images and quality assessments are conducted. The experiments show that our method is effective and applicable for house identification. The proposed ERI and SLI features can improve the precision and recall by 5.6% and 11.2%, respectively.