bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of Syngas Bioconversion to Value-Added Products from 2012 to 2022
Authors: Pacheco Marta , Moura Patrícia , Silva Carla
Published: April 2023

Synthesis gas (syngas) fermentation is a biological carbon fixation process through which carboxydotrophic acetogenic bacteria convert CO, CO2, and H2 into platform chemicals. To obtain an accurate overview of the syngas fermentation research and innovation from 2012 to 2022, a systematic search was performed on Web of Science and The Lens, focusing on academic publications and patents that were published or granted during this period. Overall, the research focus was centered on process optimization, the genetic manipulation of microorganisms, and bioreactor design, in order to increase the plethora of fermentation products and expand their possible applications. Most of the published research was initially funded and developed in the United States of America. However, over the years, European countries have become the major contributors to syngas fermentation research, followed by China. Syngas fermentation seems to be developing at “two-speeds”, with a small number of companies controlling the technology that is needed for large-scale applications, while academia still focuses on low technology readiness level (TRL) research. This systematic review also showed that the fermentation of raw syngas, the effects of syngas impurities on acetogen viability and product distribution, and the process integration of gasification and fermentation are currently underdeveloped research topics, in which an investment is needed to achieve technological breakthroughs.
Second-Life Battery Capacity Estimation and Method Comparison
Authors: Yang Jingxi , Beatty Matthew , Strickland Dani , Abedi-Varnosfaderani Mina , Warren Joe
Published: April 2023

There is increased talk about using second-life batteries in applications. In first-life applications, the batteries start from new, and a range of life cycle estimation techniques are applied. However, it is not clear how second-life batteries should be monitored compared to first life batteries. This paper investigated different algorithms from first-life applications for estimating and forecasting battery cell state of health in conjunction with capacity calculations using second life cells under long term durability testing. The paper looks at how close these models predict capacity fade based on a set of second-life batteries that have been undertaking sweat testing over six different applications. The paper concludes that there are two methods that could be suitable candidates for predicting lifespan. One of these needed to be modified from the original.
Study of the Failure Mechanism of an Integrated Injection-Production String in Thermal Recovery Wells for Heavy Oil
Authors: Zhang Wei , Gao Deli , Liu Yigang , Bai Jianhua , Wang Cheng
Published: April 2023

The integrated injection-production string is the core tool used in thermal recovery wells for heavy oil, the mechanical behavior of which is complex due to the coupling effect of downhole temperature and pressure and the load induced by steam huff and puff operations. In this paper, an analysis model that calculates the temperature and pressure field during steam huff and puff operations has been established based on the basic principles of energy conservation and heat transfer. Then, the force distribution and strength check of the integrated injection-production string were analyzed. The fatigue damage of the string was evaluated by considering dynamic loads during the injection process. The corrosion life of the string was predicted. Finally, the failure mechanism of the integrated injection-production string in thermal recovery wells for heavy oil was illustrated. The results showed that the strength of the string and vibration fatigue are not the main causes of failure, but corrosion is the main reason for string failure. The residual strength of the corroded string was greatly reduced, which is the fundamental reason for the failure of the string. This paper is of guiding significance to the optimization of design and safety evaluations of the integrated injection-production string in thermal recovery wells for heavy oil.
Design and Optimization of Photovoltaic System in Full-Chain Ground-Based Validation System of Space Solar Power Station
Authors: Mi Jianwei , Du Jie , Liu Chengjian , Li Xintong , Zhang Yiqun , Fan Guanheng
Published: April 2023

In the face of the increasing depletion of non-renewable energy sources and increasingly serious environmental problems, the development of green and environmentally friendly renewable energy sources cannot be delayed. Because of the far-reaching development potential of solar energy, solar power has become an important research object for power development. The available solar energy in space is several times greater than that on Earth. Solar energy from space can be collected by a space solar power station (SSPS) and transmitted to the ground by wireless power transfer. In the full-chain ground-based validation system of SSPS-OMEGA, the spherical concentrator is used, and the light intensity distribution on the solar receiver is non-uniform. The non-uniform light intensity makes the output current of each photovoltaic (PV) cell on the solar receiver greatly different, and causes power losses, known as the mismatch problem. This paper proposes a simple, efficient and easy-to-implement method to optimize the structure of PV arrays to reduce the effect of non-uniform light on the output performance of each PV cell, which is beneficial to the topology of PV arrays and also effectively improves the layout rate. Then, a differential power processing (DPP) converter with a simple structure and easy control is designed to further deal with the power mismatch problem between series-connected PV modules. Finally, a simulation circuit model and a physical hardware model of the differential power processing PV system are built and used in the full-chain ground-based validation system of SSPS-OMEGA. The results demonstrate that the influence of non-uniform lighting on PV cells is effectively reduced, the output power of PV modules connected in series under non-uniform light distribution is substantially increased, and the photoelectric conversion efficiency is significantly improved.
Identification of Key Events and Emissions during Thermal Abuse Testing on NCA 18650 Cells
Authors: Ubaldi Sofia , Conti Marco , Marra Francesco , Russo Paola
Published: April 2023

Thermal abuse of lithium-ion batteries (LIBs) leads to the emission of gases, solids, fires and/or explosions. Therefore, it is essential to define the temperatures at which key events occur (i.e., CID activation, venting, and thermal runaway (TR)) and to identify the related emissions for identifying the hazards to which people and especially rescue teams are exposed. For this purpose, thermal abuse tests were performed on commercial lithium nickel cobalt aluminum oxide (NCA) 18650 cells at 50% state of charge in a reactor connected to an FT-IR spectrometer by varying test conditions (feed gas of N2 or air; heating rates of 5 or 10 °C/min until 300 °C). In particular, the concentrations of the gases and the composition of the condensed-phase emissions were estimated. As regards gases, a high concentration (1695 ppmv) of hydrofluoric acid (HF) was measured, while the emissions of condensed matter consisted of organic compounds such as polyethylene oxide and paraffin oil, and inorganic compounds containing Li (0.173 mg/m3) and Al (0.344 mg/m3). The main safety concerns were caused by the temperatures (564 ± 85 °C) reached by the cell during TR, by the HF concentration which exceeded the toxicity limits of 30 ppm, the IDLH defined by the NIOSH, and the diameter of the particles (1.54 ± 0.69 µm) that rose the PM2.5 concentration. These results are also useful for identifying personal protection equipment for rescue teams.
A Comprehensive Review on Planar Magnetics and the Structures to Reduce the Parasitic Elements and Improve Efficiency
Authors: Kolahian Pouya , Grimm Ferdinand , Baghdadi Mehdi
Published: April 2023

Due to the need for highly efficient and compact power electronic converters to operate at higher frequencies, traditional wire-wound magnetics are not suitable. This paper provides a comprehensive review of planar magnetic technologies, discussing their advantages as well as associated disadvantages. An extensive review of the research literature is presented with the aim of suggesting models for planar magnetics. Several strategies are proposed to overcome the limitations of planar magnetics, including winding conduction loss, leakage inductance, and winding capacitance. The goal of this study is to provide engineers and researchers with a clear roadmap for designing planar magnetic devices.
Chemical Elements Content and Distributions within Different Tissue Types of White Spruce
Authors: Mvolo Cyriac S. , Boakye Emmanuel A. , Koubaa Ahmed
Published: April 2023

The relative proportions of different chemical components in wood tissues is one of the underlying factors that control wood properties. These proportions vary within and between woody tissues, and an accurate description of these variations is critical for parameterizing forest biogeochemical budgets and models. White spruce (Picea glauca (Moench) Voss) spacing intensities trials in the Petawawa Research Forest, Ontario, Canada, were sampled to evaluate variations in carbon (C), nitrogen (N), and hydrogen (H) concentrations between different tissue types, i.e., bark, cambium, knots, earlywood, latewood, and wood. Samples were freeze-dried and oven-dried to test the impact of the drying methods on these chemical elements. Freeze-dried C (51.14) and H (6.18) concentrations were significantly higher than those of oven-dried C (50.55) and H (6.06). Freeze-dried N (0.18) did not differ from oven-dried N (0.17). The spacing intensities impacted C, H, and N, with C content being higher in wider square spacings (4.3 m and 6.1 m), while the reverse was true for N and H, which exhibited higher content in smaller square spacings (1.2 m and 1.8 m). The results of this study also suggested that when it comes to the content of chemical elements, bark and knots should be treated as separate fuel types, whereas other woody tissues can be aggregated.
Heat Transfer and Heat Recovery Systems
Authors: Rajski Krzysztof , Danielewicz Jan
Published: April 2023

Heat transfer is present in all modern engineering applications and plays a fundamental role in improving the efficiency of energy conversion systems [...]
Optimization of the Production and Characterization of an Antifungal Protein by Bacillus velezensis Strain NT35 and Its Antifungal Activity against Ilyonectria robusta Causing Ginseng Rusty Root Rot
Authors: Li Mengtao , Tang Hao , Li Zongyan , Song Yu , Chen Lin , Ran Chao , Jiang Yun , Chen Changqing
Published: April 2023

A biocontrol Bacillus velezensis strain, NT35, was isolated from the rhizosphere soil of ginseng, and its sterile filtrate was obtained through a 0.22 μm filter which had a significant inhibitory effect against Ilyonectria robusta, which causes rusty root rot in Panax ginseng. In order to obtain the best sterile filtrate, the medium fermentation conditions of the strain NT35 were optimized using response surface methodology (RSM), and the best composition was obtained. Therefore, the fermentation medium was composed of yeast extract powder 2.5%, cornmeal 1.5%, K2HPO4 1.5%, and (NH4)2SO4 2.5%. The optimal inoculum amount was 6%, at an initial pH value of 7.0 and culturing at 34 °C at 180 rpm. The antifungal protein 1-4-2F was obtained through precipitation via 30% (NH4)2SO4 precipitation, desalting by Sephadex G-25, ion-exchange chromatography, and gel filtration chromatography. Tricine-SDS-PAGE showed that the purified protein had a relative molecular weight of approximately 6.5 kDa. The protein 1-4-2F was relatively stable and had better antifungal activity at pH 4–10 and 20–100 °C under ultraviolet irradiation of 30 W. The amino acid sequence of protein 1-4-2F was obtained using mass spectrometry, and had 100% similarity to a hypothetical protein from B. velezensis YAU B9601-Y2 (Accession No: AFJ62117). Its molecular weight was 10.176 kDa, the isoelectric point was 9.08, and its sequence coverage reached 49%. The EC50 value of the protein 1-4-2F against I. robusta was 1.519 μg·mL−1. The mycelia morphology of I. robusta changed significantly after treatment with antifungal protein under microscopic observation; the branches of the mycelia increased, distorted, partially swelled into a spherical or elliptical shape, and even ruptured; and the cells became vacuoles.
Emerging Technologies and Their Link to Digital Competence in Teaching
Authors: García-Delgado Miguel Ángel , Rodríguez-Cano Sonia , Delgado-Benito Vanesa , Lozano-Álvarez María
Published: April 2023

The new educational reality requires teachers to have a series of skills and competences that allow them to improve the teaching–learning process and therefore the quality of teaching, integrating technology and emerging technologies. In order to assess the competence level of teachers, a descriptive study was designed, in which 101 teachers from different stages and geographical locations in Spain took part and were administered the DigCompEdu Check-in questionnaire. The results show average levels of teachers’ digital competence (B1 and B2, particularly), and an incipient use of emerging technologies by teachers, with less than 50% of the sample not using these technologies in their daily classroom activities, although those who show a higher level of digital competence are also those who integrate them more in their daily work. The results correspond with similar studies, corroborating the average level of teachers’ digital skills.
Antioxidant Capacity and Phenolics Profile of Portuguese Traditional Cultivars of Apples and Pears and Their By-Products: On the Way to Newer Applications
Authors: Teixeira João David , Soares Mateus Ana Rita , Sanchez Claudia , Parpot Pier , Almeida Carina , Sanches Silva Ana
Published: April 2023

Pears (Pyrus communis L.) and apples (Malus domestica Borkh.) are two of the most popular fruits worldwide. The phenolic compounds they offer are associated with human health benefits due to their antioxidant properties. Since these fruits’ by-products are not yet fully exploited, it is important to characterize them, especially in terms of their antioxidant properties. The aim of this study was to determine the antioxidant properties of old traditional cultivars, six regional pear cultivars and five regional apple cultivars grown in the Alcobaça region (Portugal). Antioxidant capacity assays were used to evaluate the antioxidant properties. Generally, the antioxidant capacity, total phenolics content (TPC), and total flavonoids content (TFC) of fruit byproducts (both seeds and peels) were higher than the corresponding mesocarp, indicating their potential as sources of beneficial antioxidant compounds. Moreover, a UHPLC-ToF-MS method was optimized and validated in order to quantify 21 distinct phenolics in these fruit samples. The analytical method’s suitability for quantifying phenolic compounds was demonstrated by an evaluation of linearity, limit of detection, limit of quantification, precision and accuracy. This method was used to determine the phenolic composition of samples of regional (local) cultivars. The phenolics in the fruit samples with the highest concentrations were phlorizin and chlorogenic acid. Principal component analysis (PCA) was used to separate distinct fruit species while emphasizing their similarities and differences.
Application of Unsupervised Machine Learning for the Evaluation of Aerogels’ Efficiency towards Ion Removal—A Principal Component Analysis (PCA) Approach
Authors: Younes Khaled , Kharboutly Yahya , Antar Mayssara , Chaouk Hamdi , Obeid Emil , Mouhtady Omar , Abu-samha Mahmoud , Halwani Jalal , Murshid Nimer
Published: April 2023

Water scarcity is a global problem affecting millions of people. It can lead to severe economic, social, and environmental consequences. It can also have several impacts on agriculture, industry, and households, leading to a decrease in human quality of life. To address water scarcity, governments, communities, and individuals must work in synergy for the sake of water resources conservation and the implementation of sustainable water management practices. Following this urge, the enhancement of water treatment processes and the development of novel ones is a must. Here, we have investigated the potential of the applicability of “Green Aerogels” in water treatment’s ion removal section. Three families of aerogels originating from nanocellulose (NC), chitosan (CS), and graphene (G) are investigated. In order to reveal the difference between aerogel samples in-hand, a “Principal Component Analysis” (PCA) has been performed on the physical/chemical properties of aerogels, from one side, and the adsorption features, from another side. Several approaches and data pre-treatments have been considered to overcome any bias of the statistical method. Following the different followed approaches, the aerogel samples were located in the center of the biplot and were surrounded by different physical/chemical and adsorption properties. This would probably indicate a similar efficiency in the ion removal of the aerogels in-hand, whether they were nanocellulose-based, chitosan-based, or even graphene-based. In brief, PCA has shown a similar efficiency of all the investigated aerogels towards ion removal. The advantage of this method is its capacity to engage and seek similarities/dissimilarities between multiple factors, with the elimination of the shortcomings for the tedious and time-consuming bidimensional data visualization.
Leishmania infantum (JPCM5) Transcriptome, Gene Models and Resources for an Active Curation of Gene Annotations
Authors: Camacho Esther , González-de la Fuente Sandra , Solana Jose Carlos , Tabera Laura , Carrasco-Ramiro Fernando , Aguado Begoña , Requena Jose M.
Published: April 2023

Leishmania infantum is one of the causative agents of visceral leishmaniases, the most severe form of leishmaniasis. An improved assembly for the L. infantum genome was published five years ago, yet delineation of its transcriptome remained to be accomplished. In this work, the transcriptome annotation was attained by a combination of both short and long RNA-seq reads. The good agreement between the results derived from both methodologies confirmed that transcript assembly based on Illumina RNA-seq and further delimitation according to the positions of spliced leader (SAS) and poly-A (PAS) addition sites is an adequate strategy to annotate the transcriptomes of Leishmania, a procedure previously used for transcriptome annotation in other Leishmania species and related trypanosomatids. These analyses also confirmed that the Leishmania transcripts boundaries are relatively slippery, showing extensive heterogeneity at the 5′- and 3′-ends. However, the use of RNA-seq reads derived from the PacBio technology (referred to as Iso-Seq) allowed the authors to uncover some complex transcription patterns occurring at particular loci that would be unnoticed by the use of short RNA-seq reads alone. Thus, Iso-Seq analysis provided evidence that transcript processing at particular loci would be more dynamic than expected. Another noticeable finding was the observation of a case of allelic heterozygosity based on the existence of chimeric Iso-Seq reads that might be generated by an event of intrachromosomal recombination. In addition, we are providing the L. infantum gene models, including both UTRs and CDS regions, that would be helpful for undertaking whole-genome expression studies. Moreover, we have built the foundations of a communal database for the active curation of both gene/transcript models and functional annotations for genes and proteins.
Improved RAPD Method for Candida parapsilosis Fingerprinting
Authors: Wojciechowska-Koszko Iwona , Mnichowska-Polanowska Magdalena , Roszkowska Paulina , Sławiński Michał , Giedrys-Kalemba Stefania , Dołęgowska Barbara , Sienkiewicz Monika , Hukowska-Szematowicz Beata , Kwiatkowski Paweł
Published: April 2023

Recently, methods based on the analysis of arbitrarily amplified target sites of genome microorganisms have been extensively applied in microbiological studies, and especially in epidemiological studies. The range of their application is limited by problems with discrimination and reproducibility resulting from a lack of standardized and reliable methods of optimization. The aim of this study was to obtain optimal parameters of the Random Amplified Polymorphic DNA (RAPD) reaction by using an orthogonal array as per the Taguchi and Wu protocol, modified by Cobb and Clark for Candida parapsilosis isolates. High Simpson’s index values and low Dice coefficients obtained in this study indicated a high level of interspecies DNA polymorphism between C. parapsilosis strains, and the optimized RAPD method proved useful in the microbiological and epidemiological study.
The Influence of Methyl Jasmonate on Expression Patterns of Rosmarinic Acid Biosynthesis Genes, and Phenolic Compounds in Different Species of Salvia subg. Perovskia Kar L.
Authors: Kianersi Farzad , Amin Azarm Davood , Fatemi Farzaneh , Jamshidi Bita , Pour-Aboughadareh Alireza , Janda Tibor
Published: April 2023

Salvia yangii B.T. Drew and Salvia abrotanoides Kar are two important fragrant and medicinal plants that belong to the subgenus Perovskia. These plants have therapeutic benefits due to their high rosmarinic acid (RA) content. However, the molecular mechanisms behind RA generation in two species of Salvia plants are still poorly understood. As a first report, the objectives of the present research were to determine the effects of methyl jasmonate (MeJA) on the rosmarinic acid (RA), total flavonoid and phenolic contents (TFC and TPC), and changes in the expression of key genes involved in their biosynthesis (phenylalanine ammonia lyase (PAL), 4-coumarate-CoA ligase (4CL), and rosmarinic acid synthase (RAS)). The results of High-performance liquid chromatography (HPLC) analysis indicated that MeJA significantly increased RA content in S. yungii and S. abrotanoides species (to 82 and 67 mg/g DW, respectively) by 1.66- and 1.54-fold compared with untreated plants. After 24 h, leaves of Salvia yangii and Salvia abrotanoides species treated with 150 M MeJA had the greatest TPC and TFC (80 and 42 mg TAE/g DW, and 28.11 and 15.14 mg QUE/g DW, respectively), which was in line with the patterns of gene expression investigated. Our findings showed that MeJA dosages considerably enhanced the RA, TPC, and TFC contents in both species compared with the control treatment. Since increased numbers of transcripts for PAL, 4CL, and RAS were also detected, the effects of MeJA are probably caused by the activation of genes involved in the phenylpropanoid pathway.
Review of the Current Research Progress of Seed Germination Inhibitors
Authors: Chenyin Peng , Yu Wu , Fenghou Shi , Yongbao Shen
Published: April 2023

Germination inhibitors, which inhibit the germination of seeds, spores and other plant reproductive material, are abundant in the plant kingdom and include phenols, cyanides, alkaloids, essential oils, amino acids, etc. These inhibitors can be classified as germination destructors and germination retarders depending on whether they harm the morphology, structure and physiology of the seed. Germination retarders are closely related to seed dormancy, and exogenous retarders can be used to extend the “dormancy” period of non-dormant seeds or perishable seeds by applying the proper dosage. They have significant potential applications as preservatives for seed preservation following harvest or for the storage of long-term germplasm resources. Germination destructors, as a type of relatively high-efficiency, low-specificity “toxic chemicals”, are of significant benefit in the application of effective and environmentally benign herbicides. At present, the main problems related to the research methods of germination inhibitors include difficulty in determining the specific endogenous substances and the minimum inhibitory concentration to induce dormancy, as well as whether the application of exogenous inhibitors will cause physiological damage to seeds. In the future, we should strengthen the tracking of germination inhibitors, explore the mechanisms of action of specific substances and deeper molecular mechanisms and finally explore new developments and new applications of different inhibitors.
How do Informal Caregivers of Seniors’ Tasks Lead to Presenteeism and Absenteeism Behaviors? A Canadian Quantitative Study
Authors: Beauchamp Legault Marie-Ève , Chênevert Denis , Maisonneuve Francis , Mansour Sari
Published: April 2023

This study extends our knowledge on the role of informal caregivers of seniors and the impact of this role on presenteeism and absenteeism at work. Based on the conservation of resources theory, this article seeks to gain insights into the mechanisms and antecedents of presenteeism and absenteeism among employees who are also informal caregivers of seniors. Specifically, this article argues that family–work conflict and emotional exhaustion mediate the relationship between the informal caregiver’s role, presenteeism, and absenteeism. Quantitative data (questionnaire) from this cross-sectional study were collected from 915 informal caregivers of seniors from 8 Canadian organizations. Structural equation modelling (SEM) was undertaken using IBM SPSS AMOS 28.0 to test all hypotheses. Informal caregivers of seniors who need to coordinate and organize healthcare are at a higher risk of experiencing family–work conflict. Family–work conflict experienced by informal caregivers subsequently leads to emotional exhaustion, presenteeism, and absenteeism. Because informal caregiving of seniors is likely to increase in coming years for many workers, organizations must be aware of the possible consequences of this role on work productivity. This study shows that not all tasks of informal caregivers of older adults lead to presenteeism and absenteeism through family–work conflict and emotional exhaustion. This study is innovative because, to our knowledge, no study of informal caregivers of older adults has examined the effect of different tasks in this role on presenteeism and absenteeism.
Adaptation Resources and Responses to Wildfire Smoke and Other Forms of Air Pollution in Low-Income Urban Settings: A Mixed-Methods Study
Authors: Palinkas Lawrence A. , De Leon Jessenia , Yu Kexin , Salinas Erika , Fernandez Cecilia , Johnston Jill , Rahman Md Mostafijur , Silva Sam J. , Hurlburt Michael , McConnell Rob S. , Garcia Erika
Published: April 2023

Little is known about how low-income residents of urban communities engage their knowledge, attitudes, behaviors, and resources to mitigate the health impacts of wildfire smoke and other forms of air pollution. We interviewed 40 adults in Los Angeles, California, to explore their threat assessments of days of poor air quality, adaptation resources and behaviors, and the impacts of air pollution and wildfire smoke on physical and mental health. Participants resided in census tracts that were disproportionately burdened by air pollution and socioeconomic vulnerability. All participants reported experiencing days of poor air quality due primarily to wildfire smoke. Sixty percent received advanced warnings of days of poor air quality or routinely monitored air quality via cell phone apps or news broadcasts. Adaptation behaviors included remaining indoors, circulating indoor air, and wearing face masks when outdoors. Most (82.5%) of the participants reported some physical or mental health problem or symptom during days of poor air quality, but several indicated that symptom severity was mitigated by their adaptive behaviors. Although low-income residents perceive themselves to be at risk for the physical and mental health impacts of air pollution, they have also adapted to that risk with limited resources.
From Deficit to Strength-Based Aboriginal Health Research—Moving toward Flourishing
Authors: Bullen Jonathan , Hill-Wall Trish , Anderson Kate , Brown Alex , Bracknell Clint , Newnham Elizabeth A. , Garvey Gail , Waters Lea
Published: April 2023

Aboriginal Australians have a fundamental human right to opportunities that lead to healthy and flourishing lives. While the impact of trauma on Aboriginal Australians is well-documented, a pervasive deficit narrative that focuses on problems and pathology persists in research and policy discourse. This narrative risks further exacerbating Aboriginal disadvantage through a focus on ‘fixing what is wrong’ with Aboriginal Australians and the internalising of these narratives by Aboriginal Australians. While a growing body of research adopts strength-based models, limited research has sought to explore Aboriginal flourishing. This conceptual paper seeks to contribute to a burgeoning paradigm shift in Aboriginal research, seeking to understand what can be learned from Aboriginal people who flourish, how we best determine this, and in what contexts this can be impactful. Within, we argue the case for a new approach to exploring Aboriginal wellbeing that integrates salutogenic, positive psychology concepts with complex systems theory to understand and promote Aboriginal wellbeing and flourishing. While deeper work may be required to establish the parameters of a strength-based, culturally aligned Aboriginal conceptualisation of positive psychology, we suggest the integration of Aboriginal and Western methodologies offers a unique and potent means of shifting the dial on seemingly intractable problems.
Cell Phone Radiation Exposure Limits and Engineering Solutions
Authors: Héroux Paul , Belyaev Igor , Chamberlin Kent , Dasdag Suleyman , De Salles Alvaro Augusto Almeida , Rodriguez Claudio Enrique Fernandez , Hardell Lennart , Kelley Elizabeth , Kesari Kavindra Kumar , Mallery-Blythe Erica , Melnick Ronald L. , Miller Anthony B. , Moskowitz Joel M. ,   on behalf of the International Commission on the Biological Effects of Electromagnetic Fields (ICBE-EMF)
Published: April 2023

In the 1990s, the Institute of Electrical and Electronics Engineers (IEEE) restricted its risk assessment for human exposure to radiofrequency radiation (RFR) in seven ways: (1) Inappropriate focus on heat, ignoring sub-thermal effects. (2) Reliance on exposure experiments performed over very short times. (3) Overlooking time/amplitude characteristics of RFR signals. (4) Ignoring carcinogenicity, hypersensitivity, and other health conditions connected with RFR. (5) Measuring cellphone Specific Absorption Rates (SAR) at arbitrary distances from the head. (6) Averaging SAR doses at volumetric/mass scales irrelevant to health. (7) Using unrealistic simulations for cell phone SAR estimations. Low-cost software and hardware modifications are proposed here for cellular phone RFR exposure mitigation: (1) inhibiting RFR emissions in contact with the body, (2) use of antenna patterns reducing the Percent of Power absorbed in the Head (PPHead) and body and increasing the Percent of Power Radiated for communications (PPR), and (3) automated protocol-based reductions of the number of RFR emissions, their duration, or integrated dose. These inexpensive measures do not fundamentally alter cell phone functions or communications quality. A health threat is scientifically documented at many levels and acknowledged by industries. Yet mitigation of RFR exposures to users does not appear as a priority with most cell phone manufacturers.
Potential Relationship between Poor Oral Hygiene and MRONJ: An Observational Retrospective Study
Authors: D’Agostino Silvia , Valentini Giulia , Dolci Marco , Ferrara Elisabetta
Published: April 2023

Medication-related osteonecrosis of the jaw (MRONJ) is a drug-related side effect linked but not limited to antiresorptive and antiangiogenic molecules. It recognizes several triggers in dental procedures, such as surgery, endodontic treatments, and root planing, but also prosthesis decubitus or with a spontaneous onset. Although there are many reports about the onset of this pathology, oral hygiene status is mainly described as a consequence of MRONJ. Not so much is known about the oral hygiene situation as a concurrent factor in the pathogenesis of severe stages and about non-surgical periodontal therapy in patients affected by MRONJ. Actually, clear instructions for non-surgical periodontal therapy are poor in the literature. The primary outcome of the present study is to evaluate the oral hygiene status in MRONJ patients. In addition, a secondary outcome is to review the factor of poor oral hygiene as a cause or worsening aspect for MRONJ. A total of 45 subjects (19 males and 26 females) with a mean age of 59 ± 12 were enrolled. The Pearson correlation coefficient showed no significant results for the variable of the Simplified Oral Hygiene Index (OHI-S) and the American Association of Oral and Maxillofacial Surgeons (AAOMS) stage, although the majority of patients showed poor oral hygiene with an OHI-S average of 3.39 ± 1.83. As stated by the last AAOMS position paper, poor plaque control is related to a worsened MRONJ stage. The relation between the lack of oral hygiene and MRONJ onset is still unclear.
New Insight into the Genotype-Phenotype Correlation of PRPH2-Related Diseases Based on a Large Chinese Cohort and Literature Review
Authors: Wang Yingwei , Wang Junwen , Jiang Yi , Zhu Di , Ouyang Jiamin , Yi Zhen , Li Shiqiang , Jia Xiaoyun , Xiao Xueshan , Sun Wenmin , Wang Panfeng , Zhang Qingjiong
Published: April 2023

Variants in PRPH2 are a common cause of inherited retinal dystrophies with high genetic and phenotypic heterogeneity. In this study, variants in PRPH2 were selected from in-house exome sequencing data, and all reported PRPH2 variants were evaluated with the assistance of online prediction tools and the comparative validation of large datasets. All variants were classified based on the American College of Medical Genetics and Genomics/Association for Molecular Pathology (ACMG/AMP) guidelines. Individuals with pathogenic or likely pathogenic variants of PRPH2 were confirmed by Sanger sequencing. Clinical characteristics were summarized. Ten pathogenic or likely pathogenic variants of PRPH2 were identified in 14 families. In our cohort, the most frequent variant was p.G305Afs*19, accounting for 33.3% (5/15) of alleles, in contrast to the literature, where p.R172G (11.6%, 119/1028) was the most common variant. Nine in-house families (63.8%) were diagnosed with retinitis pigmentosa (RP), distinct from the phenotypic spectrum in the literature, which shows that RP accounts for 27.9% (283/1013) and macular degeneration is more common (45.2%, 458/1013). Patients carrying missense variants predicted as damaging by all seven prediction tools and absent in the gnomAD database were more likely to develop RP compared to those carrying missense variants predicted as damaging with fewer tools or with more than one allele number in the gnomAD database (p = 0.001). The population-specific genetic and phenotypic spectra of PRPH2 were explored, and novel insight into the genotype–phenotype correlation of PRPH2 was proposed. These findings demonstrated the importance of assessing PRPH2 variants in distinct populations and the value of providing practical suggestions for the genetic interpretation of PRPH2 variants.
Lung Epithelial Cells from Obese Patients Have Impaired Control of SARS-CoV-2 Infection
Authors: Gaudet Mellissa , Kaufmann Eva , Jalaleddine Nour , Mogas Andrea , Hachim Mahmood , Senok Abiola , Divangahi Maziar , Hamid Qutayba , Al Heialy Saba
Published: April 2023

Obesity is known to increase the complications of the COVID-19 coronavirus disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the exact mechanisms of SARS-CoV-2 infection in obese patients have not been clearly elucidated. This study aims to better understand the effect of obesity on the course of SARS-CoV-2 infection and identify candidate molecular pathways involved in the progression of the disease, using an in vitro live infection model and RNA sequencing. Results from this study revealed the enhancement of viral load and replication in bronchial epithelial cells (NHBE) from obese subjects at 24 h of infection (MOI = 0.5) as compared to non-obese subjects. Transcriptomic profiling via RNA-Seq highlighted the enrichment of lipid metabolism-related pathways along with LPIN2, an inflammasome regulator, as a unique differentially expressed gene (DEG) in infected bronchial epithelial cells from obese subjects. Such findings correlated with altered cytokine and angiotensin-converting enzyme-2 (ACE2) expression during infection of bronchial cells. These findings provide a novel insight on the molecular interplay between obesity and SARS-CoV-2 infection. In conclusion, this study demonstrates the increased SARS-CoV-2 infection of bronchial epithelial cells from obese subjects and highlights the impaired immunity which may explain the increased severity among obese COVID-19 patients.
Interferons and Resistance Mechanisms in Tumors and Pathogen-Driven Diseases—Focus on the Major Histocompatibility Complex (MHC) Antigen Processing Pathway
Authors: Massa Chiara , Wang Yuan , Marr Nico , Seliger Barbara
Published: April 2023

Interferons (IFNs), divided into type I, type II, and type III IFNs represent proteins that are secreted from cells in response to various stimuli and provide important information for understanding the evolution, structure, and function of the immune system, as well as the signaling pathways of other cytokines and their receptors. They exert comparable, but also distinct physiologic and pathophysiologic activities accompanied by pleiotropic effects, such as the modulation of host responses against bacterial and viral infections, tumor surveillance, innate and adaptive immune responses. IFNs were the first cytokines used for the treatment of tumor patients including hairy leukemia, renal cell carcinoma, and melanoma. However, tumor cells often develop a transient or permanent resistance to IFNs, which has been linked to the escape of tumor cells and unresponsiveness to immunotherapies. In addition, loss-of-function mutations in IFN signaling components have been associated with susceptibility to infectious diseases, such as COVID-19 and mycobacterial infections. In this review, we summarize general features of the three IFN families and their function, the expression and activity of the different IFN signal transduction pathways, and their role in tumor immune evasion and pathogen clearance, with links to alterations in the major histocompatibility complex (MHC) class I and II antigen processing machinery (APM). In addition, we discuss insights regarding the clinical applications of IFNs alone or in combination with other therapeutic options including immunotherapies as well as strategies reversing the deficient IFN signaling. Therefore, this review provides an overview on the function and clinical relevance of the different IFN family members, with a specific focus on the MHC pathways in cancers and infections and their contribution to immune escape of tumors.
Evolution-Informed Strategies for Combating Drug Resistance in Cancer
Authors: Lin-Rahardja Kristi , Weaver Davis T. , Scarborough Jessica A. , Scott Jacob G.
Published: April 2023

The ever-changing nature of cancer poses the most difficult challenge oncologists face today. Cancer’s remarkable adaptability has inspired many to work toward understanding the evolutionary dynamics that underlie this disease in hopes of learning new ways to fight it. Eco-evolutionary dynamics of a tumor are not accounted for in most standard treatment regimens, but exploiting them would help us combat treatment-resistant effectively. Here, we outline several notable efforts to exploit these dynamics and circumvent drug resistance in cancer.
Inter-Alpha Inhibitor Proteins Modify the Microvasculature after Exposure to Hypoxia–Ischemia and Hypoxia in Neonatal Rats
Authors: Girolamo Francesco , Lim Yow-Pin , Virgintino Daniela , Stonestreet Barbara S. , Chen Xiaodi F.
Published: April 2023

Microvasculature develops during early brain development. Hypoxia–ischemia (HI) and hypoxia (H) predispose to brain injury in neonates. Inter-alpha inhibitor proteins (IAIPs) attenuate injury to the neonatal brain after exposure to HI. However, the effects of IAIPs on the brain microvasculature after exposure to HI have not been examined in neonates. Postnatal day-7 rats were exposed to sham treatment or right carotid artery ligation and 8% oxygen for 90 min. HI comprises hypoxia (H) and ischemia to the right hemisphere (HI-right) and hypoxia to the whole body, including the left hemisphere (H-left). Human IAIPs (hIAIPs, 30 mg/kg) or placebo were injected immediately, 24 and 48 h after HI/H. The brains were analyzed 72 h after HI/H to determine the effects of hIAIPs on the microvasculature by laminin immunohistochemistry and calculation of (1) the percentage area stained by laminin, (2) cumulative microvessel length, and (3) density of tunneling nanotubes (TNTs), which are sensitive indicators of the earliest phases of neo-vascularization/collateralization. hIAIPs mainly affected the percent of the laminin-stained area after HI/H, cumulative vessel length after H but not HI, and TNT density in females but not males. hIAIPs modify the effects of HI/H on the microvasculature after brain injury in neonatal rats and exhibit sex-related differential effects. Our findings suggest that treatment with hIAIPs after exposure to H and HI in neonatal rats affects the laminin content of the vessel basal lamina and angiogenic responses in a sex-related fashion.
Sevoflurane Induces a Cyclophilin D-Dependent Decrease of Neural Progenitor Cells Migration
Authors: Lu Pan , Liang Feng , Dong Yuanlin , Xie Zhongcong , Zhang Yiying
Published: April 2023

Clinical studies have suggested that repeated exposure to anesthesia and surgery at a young age may increase the risk of cognitive impairment. Our previous research has shown that sevoflurane can affect neurogenesis and cognitive function in young animals by altering cyclophilin D (CypD) levels and mitochondrial function. Neural progenitor cells (NPCs) migration is associated with cognitive function in developing brains. However, it is unclear whether sevoflurane can regulate NPCs migration via changes in CypD. To address this question, we treated NPCs harvested from wild-type (WT) and CypD knockout (KO) mice and young WT and CypD KO mice with sevoflurane. We used immunofluorescence staining, wound healing assay, transwell assay, mass spectrometry, and Western blot to assess the effects of sevoflurane on CypD, reactive oxygen species (ROS), doublecortin levels, and NPCs migration. We showed that sevoflurane increased levels of CypD and ROS, decreased levels of doublecortin, and reduced migration of NPCs harvested from WT mice in vitro and in WT young mice. KO of CypD attenuated these effects, suggesting that a sevoflurane-induced decrease in NPCs migration is dependent on CypD. Our findings have established a system for future studies aimed at exploring the impacts of sevoflurane anesthesia on the impairment of NPCs migration.
Modulation of Cellular Senescence in HEK293 and HepG2 Cells by Ultrafiltrates UPla and ULu Is Partly Mediated by Modulation of Mitochondrial Homeostasis under Oxidative Stress
Authors: Zhou Junxian , Liu Kang , Bauer Chris , Bendner Gerald , Dietrich Heike , Slivka Jakub Peter , Wink Michael , Wong Michelle B. F. , Chan Mike K. S. , Skutella Thomas
Published: April 2023

Protein probes, including ultrafiltrates from the placenta (UPla) and lung (ULu) of postnatal rabbits, were investigated in premature senescent HEK293 and HepG2 cells to explore whether they could modulate cellular senescence. Tris-Tricine–PAGE, gene ontology (GO), and LC–MS/MS analysis were applied to describe the characteristics of the ultrafiltrates. HEK293 and HepG2 cells (both under 25 passages) exposed to a sub-toxic concentration of hydrogen peroxide (H2O2, 300 μM) became senescent; UPla (10 μg/mL), ULu (10 μg/mL), as well as positive controls lipoic acid (10 μg/mL) and transferrin (10 μg/mL) were added along with H2O2 to the cells. Cell morphology; cellular proliferation; senescence-associated beta-galactosidase (SA-β-X-gal) activity; expression of senescence biomarkers including p16 INK4A (p16), p21 Waf1/Cip1 (p21), HMGB1, MMP-3, TNF-α, IL-6, lamin B1, and phospho-histone H2A.X (γ-H2AX); senescence-related gene expression; reactive oxygen species (ROS) levels; and mitochondrial fission were examined. Tris-Tricine–PAGE revealed prominent detectable bands between 10 and 100 kDa. LC–MS/MS identified 150–180 proteins and peptides in the protein probes, and GO analysis demonstrated a distinct enrichment of proteins associated with “extracellular space” and “proteasome core complex”. UPla and ULu modulated senescent cell morphology, improved cell proliferation, and decreased beta-galactosidase activity, intracellular and mitochondrial ROS production, and mitochondrial fission caused by H2O2. The results from this study demonstrated that UPla and Ulu, as well as lipoic acid and transferrin, could protect HEK293 and HepG2 cells from H2O2-induced oxidative damage via protecting mitochondrial homeostasis and thus have the potential to be explored in anti-aging therapies.
Dysregulation of Resolvin E1 Metabolism and Signaling in a Light-Damage Model of Age-Related Macular Degeneration
Authors: Tisi Annamaria , Carozza Giulia , Leuti Alessandro , Maccarone Rita , Maccarrone Mauro
Published: April 2023

Resolvin E1 (RvE1) is an eicosapentaenoic acid-derived lipid mediator involved in the resolution of inflammation. Here, we investigated whether RvE1 alterations may occur in an animal model of age-related macular degeneration (AMD). To this end, Sprague Dawley albino rats underwent light damage (LD), and retinas and serum were analyzed immediately or seven days after treatment. Western blot of retinas showed that the RvE1 receptor ChemR23 and the RvE1 metabolic enzymes 5-LOX and COX-2 were unchanged immediately after LD, but they were significantly up-regulated seven days later. Instead, the RvE1 receptor BLT1 was not modulated by LD, and neither was the RvE1 degradative enzyme 15-PGDH. Moreover, ChemR23, 5-LOX, COX-2 and BLT1 were found to be more expressed in the inner retina under all experimental conditions, as observed through ImageJ plot profile analysis. Of note, amacrine cells highly expressed BLT1, while ChemR23 was highly expressed in the activated microglia of the outer retina. ELISA assays also showed that LD rats displayed significantly higher circulating levels and reduced retinal levels of RvE1 compared to controls. Altogether, our data indicate that RvE1 metabolism and signaling are modulated in the LD model, suggesting a potentially relevant role of this pathway in AMD.