bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictive value of lactate dehydrogenase to albumin ratio (LAR) in patients with coronavirus Disease 2019 (COVID-19)
Authors: Liu Mingqing , Zhang Luping , Zhang Yingli , Xu Xiaoming , Ma Tantan , Ni Fengming , Xu Hong , Zhang Nan
Published: March 2022

Coronavirus Disease-2019 (COVID-19) is an emerging acute infectious
disease around the world. Therefore, it is crucial to identify the risk
factors of in-hospital mortality and disease severity for COVID-19
patients. We firstly proposed a biomarker ratio, lactate dehydrogenase
to albumin ratio (LAR) may be more reliable to assess the predictive
value of LAR for in-hospital mortality and early identification of
critical COVID-19 patients. A retrospective study was conducted
including patients (≥18 years old) with laboratory-confirmed COVID-19
infection who had been discharged or had died from 1 February to 29
February, 2020. The study included 321 patients and the median age of
the 321 patients was 63.0 (IQR 51.0-70.0), ranging from 19 to 95 years
old and 180 (56.1%) patients were male. 142 (44.2%) patients had 1 or
more coexisting comorbidity. The most common symptoms on admission were
fever(289[90%]) and cough(258[80.4%]). In multivariable
logistic regression, only older age (OR, 1.11; 95% CI, 1.05-1.16), WBC
count (OR, 1.26; 95% CI, 1.11-1.44), lymphocyte count (OR, 0.78; 95%
CI, 0.62-0.99) and LAR (OR, 1.29; 95% CI, 1.18-1.40) were found to be
significantly associated with in-hospital death. ROC analysis showed
that LAR had a higher AUC (0.917) and the highest specificity(84.0%)
and sensitivity(84.6%). Furthermore, the results showed that LAR had a
higher AUC (0.931) to differentiate critical from mild patients and had
a sensitivity of 87.7% and a specificity of 82.1%. Besides, LAR had an
AUC (0.861) to differentiate critical from severe patients and had a
sensitivity of 86.0% and a specificity of 73.8% and the role of LAR to
distinguish severe from mild patients was the worst. To the best of our
knowledge, a high LAR appears to predict higher odds of mortality and
differentiate critical patients from mild or severe COVID-19 patients.
Pilot-scale performance of mAb-producing CHO cells using 20-nm filtered chemically-defined medium in WAVE Cellbag™ bioreactor
Authors: Manukyan Levon , Andersson Andreas , Marinaki Maria Eleni , Mantas Athanasios , Brink Charlotte , Tronnersjö Susanna , Hedberg Jesper , Mihranyan Albert
Published: March 2022

Assuring viral safety of bioreactors in upstream bioprocessing is an
important issue in manufacturing biologicals. Nanofiltration of cell
culture media components is a robust yet expensive method of assuring
biosafety. The present article investigates the performance of
mAb-producing Chinese hamster ovary (CHO) cells using 20 nm-filtered
chemically defined medium in WAVE Cellbag™ bioreactor in a 10L pilot
scale. For 20-nm virus retentive filtration nanocellulose-based
non-woven filter paper developed at Uppsala University was used. The
cell performance in WAVE Cellbag™ bioreactor was quantified with respect
to cell viability, metabolic activity, and protein yield and compared to
internal reference data from stirred tank-type bioreactor. The results
revealed that 20-nm filtered supplemented chemically-defined media
supported the growth of mAb-producing CHO cells, with desirable cell
metabolic activity and mAb yield. The observed differences between the
reference data from unfiltered media in the stirred tank bioreactor and
nanofiltered media in WAVE Cellbag™ bioreactor were mainly due to the
configuration of the equipment rather than due to nanofiltraion. The
results represent an important step forward for scaling up bioprocesses
using cost-efficient nanocellulose-based nanofilters in upstream
bioprocessing.
Impact of relative motion between magnetic field and boundary movement on time-dependent MHD Couette flow of dusty fluid in a porous annulus
Authors: Jha Basant , Gambo Dauda
Published: March 2022

The transient flow of a magnetohydrodynamic binary mixture passing
through a porous annular duct has been proposed semi-analytically.
Particularly, the role of velocity of applied magnetic field and
relative motion of the bounding wall has been investigated
theoretically. Here, our treatment of the governing momentum equations
accountable for the flow is done using the classical Laplace transform
technique and Riemann-Sum Approximation (RSA). The effects of the flow
parameters on the fluid phase velocity, dust phase velocity and skin
frictions has been illustrated pictorially. Findings suggests that a
growing time is necessary for an increasing velocity and decreasing drag
on the outer wall of the cylinder for both components of flow. Hartmann
number is seen to accelerate velocity and skin frictions for both cases
of boundary movement with an overwhelming presence of velocity of
applied magnetic field over the local Lorentz force for the fluid phase
and dust phase. Moreover, higher profiles for velocity and skin
frictions are seen with fluid phase and impulsive movement of cylinder.
Development of Ovalbumin-Lipopolysaccharide induced Vasculitis in rats - An experiment animal model
Authors: Thakur Vandana , Mehta Anita
Published: March 2022

Vasculitis is considered a hidden factor for many pathological
conditions, and thus, there is a need to develop a distinctive animal
model to aid drug testing. Studies reported that the adjunct use of
antigens, ovalbumin, and lipopolysaccharide exaggerates inflammation.
However, to date, none has established the effect of Ova and LPS
individually or in combination in vasculitis induction. The study aimed
to investigate the effect of ovalbumin and lipopolysaccharide on
vasculitis induction in rats. The rats were sensitized with Ova and
challenged with LPS. The sensitization and challenge in rats have shown
a significant increase in circulating inflammatory cells, Erythrocyte
Sedimentation Rate (ESR), Inflammatory cytokines (IL-1β, IL-6, and
TNF-α), C-reactive protein (CRP), ANCA (Anti-MPO, Anti-PR3), liver
function enzymes (AST, ALT), kidney damage markers (BUN, Creatinine) in
the serum. MMP-9 level was significantly increased in the temporal,
carotid, aorta, iliac, mesentery, and coronary arteries. We also found
the disease control group developed hematuria and proteinuria, which was
incomparable to the normal control. Furthermore, the histopathology
suggested significant neutrophil infiltration with fibrinoid necrosis,
indicating vascular injury and hyperplasia, resulting in extracellular
matrix degradation. Thus, we conclude that the ovalbumin and
lipopolysaccharide, in combination, have developed vasculitis like
conditions, which suggest OVA-LPS as a possible new experimental model
for vasculitis and could be considered in future therapeutic studies.
Relapsed RUNX1-RUNX1T1-positive acute myeloid leukemia with pseudo-Chediak-Higashi granules and C-MYC amplification
Authors: Kondo Hiroaki , Kanayama Takuyo , Matsumura Utsuki , Urata Takayo , Osone Shinya , Imamura Toshihiko , Inaba Tohru , Hosoi Hajime
Published: March 2022

A 7-year-old boy was diagnosed with RUNX1-RUNX1T1-positive acute myeloid
leukemia. Although he achieved complete remission (CR) after
conventional chemotherapy, he experienced relapse 6 months after
completing initial treatment. The cytoplasm of myeloblasts examined at
relapse contained pink giant granules. These myeloperoxidase-positive
granules were considered to be pseudo-Chediak-Higashi (PCH) granules.
Meanwhile, blasts had acquired genetic alterations such as
hypotetraploidy with RUNX1-RUNX1T1 and C-MYC amplification, and a KIT
N822K mutation. The patient underwent cord blood transplantation and
maintains a second CR. A previous report suggests that C-MYC
amplification might be associated with PCH granule formation, and our
case supports this hypothesis.
High efficient and stable Z-scheme g-C3N4/ZnCdS photocatalysts driven by visible light for practical water splitting
Authors: Yang Qianlong , He Jiandong , Zhang Yaping , Zhu Haifeng , Xing Wei , Yu Lianqing
Published: March 2022

It is extremely desirable to develop efficient and cheap water splitting
photocatalysts. An ultrahigh performance and stable water splitting
photocatalyst g-C3N4/ZnCdS was prepared by simple sintering method.
g-C3N4 nanosheets are evenly distributed around ZnCdS nanoparticles of
20-30 nm by forming C-S bond, which can accelerate carriers separation
and reduce the carrier’s life-time from 5.5 ns to 4.10 ns. The
photocatalyst has a high photocatalytic hydrogen evolution rate of 16663
μmol•h-1•g-1 under visible light illumination (λ = 400-780 nm). UV-vis
absorption spectra demonstrate that their absorption range can reach to
visible light edge of 539 nm. Furthermore, after three recycles of
photocatalyst, the hydrogen evolution stability still remains 94%. The
excellent photocatalytic performance can be attributed to the Z-scheme
energy band structure formed between g-C3N4 and ZnCdS with C-S bond by
sintering method. This work provides significant progress toward the
synthesis of cheap Z-scheme photocatalysts for practical applications.
Synthesis of(S)-omeprazole catalyzed by soybean pod peroxidase in water-in-oil microemulsions:optimization and modeling
Authors: Tang Hui , zhang yuanyuan , Deng Yashan , Li depeng , Wang zhiyong , Li huiling , Gao xin , Wang Fanye
Published: March 2022

Response surface methodology(RSM)was used to optimize the oxidizing the
omeprazole sulfide to(S)-omeprazole catalyzed by soybean pod
peroxidase(SPP) in cetyltrimethylammonium bromide
(CTAB)/isooctane/n-butyl alcohol/water water-in-oil microemulsions. With
the initial concentration of SPP of 3200 U ml-1, the conversion of the
omeprazole sulfide, the (S)-omeprazole yield and ee were 93.75%,
91.56% and 96.08%, respectively. The mechanism of asymmetric
sulfoxidations catalyzed by SPP involves three concomitant mechanisms as
follows:(1) a two-electron reduction of SPP-I, (2) a single-electron
transfer to SPP-I and (3) nonenzymatic reactions. With 5.44% of the
average relative error, a kinetic model based on the mechanisms was
established, and the SPP-catalyzed reactions including both the
two-electron reduction and the single-electron transfer mechanisms obey
ping-pong mechanism with substrate and product inhibition, while
nonenzymatic reactions follow a power law. This study has also
demonstrated the feasibility of SPP as a substitute with low cost,
excellent enantioselectivity and better thermal stability.
Immune mediators serum levels are distinctly associated with osteoporosis in male and female patients
Authors: SOUZA DANILA , Gaia Leonardo , Silva Djalma , Silva Marcos , Oliveira Carlo , Souza Ingrid , Sousa Fausto , Favaro Pedro Ivo , Pereira Sanivia , Rodrigues Virmondes , Rodrigues Denise
Published: March 2022

Introduction: Osteoporosis is a chronic and progressive disease caused
by an imbalance in the bone remodeling process. In this process,
resorption and formation are performed by osteoclasts and osteoblasts,
respectively. This delicate balance is regulated by several hormones and
cytokines. Thus, to attain a better understanding of the bone remodeling
associated process with osteoporosis in aging subjects, the following
mediators were investigated: cytokines, chemokines and
metalloproteinase-3 (MMP-3). Patients and Methods: To this end, blood
samples were collected from 30 osteoporotic patients, cytokines,
chemokines and MMP-3 were measured with the help of ELISA and CBA.
Results: Men with osteoporosis had significantly higher levels of MMP-3
and decreased levels of CCL5 than women. Women with osteoporosis had
significantly higher levels of IL-33 compared to adult males. Discussion
and conclusion: The different immunological factors seem to act
differently in osteoporosis evaluated separately in men and women. An
increase in MMP-3 and decreased CCL5 may be an important factor in
osteoporosis in men, whereas the disease seems to be associated with an
increase in IL-33 in women.
Diosmetin ameliorates glucose metabolism in KK-Ay diabetic mice through regulation of Corynebacterium glutamicum via IRS/PI3K/ AKT signaling pathway
Authors: Gong Xiaobao , Xiong Li , Bi Caihong , Zhang Baoshun
Published: March 2022

Background and Purpose: Diosmetin (Dios), a flavonoid compound with
multiple pharmacological activities. However, fewer studies have
reported its effects on diabetes. Here, we address the effect of Dios on
glucose metabolism and gut microbiota in KK-Ay diabetic mice.
Experimental Approach: Wild type C57BL/6J mice or diabetic KK-Ay mice
were treated with vehicle or Dios for one month. The liver RNA-Seq was
used to reveal the key signaling pathway interfered with Dios. The liver
16S rRNA gene sequencing was used to reveal the effects of Dios on gut
microbiota. The experiment of C. glu transplantation was used to reveal
the relationship between Dios and C. glu on glucose metabolism. Key
Results: Dios treatment significantly decreased blood glucose and
increased serum insulin concentrations. Transcriptome sequencing
analysis found that the underlining mechanism of diosmetin on T2DM by
regulating signal pathways of glucose metabolism, which was proved by
up-regulating IRS/PI3K/AKT signaling pathway to promote glycogen
synthesis and GLUT4 translocation. Besides, Dios treatment reshaped the
unbalanced gut microbiota by suppressing the ratio of
Firmicutes/Bacteroidetes and markedly increasing the richness of C. glu.
Moreover, Treatment with C. glu and Dios together can markedly
ameliorate glucose metabolism by up-regulating IRS/PI3K/AKT signaling
pathway to promoting glycogen synthesis and GLUT4 translocation.
Conclusions and Implications: Dios treatment remarkably ameliorated
glucose metabolism in KK-Ay diabetic mice by the regulation of C. glu on
IRS/PI3K/AKT signaling pathway and reshaped the unbalanced gut
microbiota. Our study provided evidence for the application of Dios to
the treatment of T2DM.
Relative bradycardia is a useful clinical feature for distinguishing COVID-19 from influenza
Authors: Takoi Hiroyuki , Kono Yuta , Kawagoe Junichiro , Kikuchi Ryota , Togashi Yuki , Abe Shinji
Published: March 2022

We retrospectively examined febrile (>38.3°C) adult
patients with microbiologically confirmed coronavirus disease (COVID-19)
(n=48) or influenza (n=63). The median pulse rate (/min)/body
temperature (°C) ratio was significantly lower for COVID-19 patients
(2.14; interquartile range, [IQR] 1.92–2.47) than for influenza
patients (2.63; IQR, 2.31–2.93; P<0.0001). The area under the
curve was 0.80 (95% confidence interval 0.71–0.88), optimal cut-off
was 2.23, and sensitivity and specificity were 66.7% and 84.1%,
respectively. Our preliminary results suggest that febrile patients with
COVID-19 exhibit more severe bradycardia than those with influenza.
Relative bradycardia is a useful feature for distinguishing COVID-19
from influenza.
Adaptive Cardiac Resynchronization Therapy Effect on Electrical Dyssynchrony (aCRT-ELSYNC): a randomized controlled trial.
Authors: Haq Kazi , Rogovoy Nichole , Thomas Jason , Hamilton Christopher , Lutz Katherine , Wirth Ashley , Bender Aron , German David , Przybylowicz Ryle , Dam Peter Van , Dewland Thomas , Dalouk Khidir , Stecker Eric , Nazer Babak , Jessel Peter , MacMurdy Karen , Zarraga Ignatius Gerardo , Beitinjaneh Bassel , Henrikson Charles , Raitt Merritt , Fuss Cristina , Ferencik Maros , Tereshchenko Larisa
Published: March 2022

Introduction: Adaptive cardiac resynchronization therapy (aCRT) is known
to have clinical benefits over conventional CRT. We aimed to compare the
effects of aCRT and conventional CRT on electrical dyssynchrony.
Methods: We conducted a double-blind, randomized controlled trial in
patients receiving CRT for routine clinical indications. Participants
underwent cardiac computed tomography and 128-electrodes body surface
mapping. We measured electrical dyssynchrony on the epicardial surface
using noninvasive electrocardiographic imaging (ECGI) before and 6
months post-CRT. Ventricular electrical uncoupling (VEU) was calculated
as the difference between the mean left ventricular (LV) and right
ventricular (RV) activation times. An electrical dyssynchrony index
(EDI) was computed as the standard deviation of local epicardial
activation times. Results: We randomized 27 participants (mean age 64±12
y; 34% female; 53% ischemic cardiomyopathy; LV ejection fraction
28±8%; QRS duration 155±21 ms; strict left bundle branch block (LBBB)
in 13%) to conventional CRT (n=15) versus aCRT (n=12). In atypical LBBB
(n=11;41%) with S-waves in V5-V6, conduction block occurred in the
anterior RV, as opposed to the interventricular groove in those who met
the strict LBBB criteria. As compared to baseline, VEU reduced post-CRT
in aCRT (median reduction 18.9 (interquartile range 4.3-29.2 ms;
P=0.034), but not in conventional CRT (21.4 (-30.0 to 49.9 ms; P=0.525)
group. There were no differences in the degree of change in VEU and EDI
indices between treatment groups. Conclusion: The effect of aCRT and
conventional CRT on electrical dyssynchrony is largely similar.
Elevated Left Atrial Volume Index Predicts Incident Atrial Fibrillation After Typical Right Atrial Flutter Ablation
Authors: Rzucidlo Justyna , Panday Priya , Lombardo Marissa , Shulman Eric , Park David , Bernstein Scott , Jankelson Lior , Holmes Douglas , Aizer Anthony , Chinitz Larry , Barbhaiya Chirag
Published: March 2022

Incident atrial fibrillation (AF) is common after cavotricuspid isthmus
(CTI) dependent atrial flutter (AFL) ablation. Risk factors for
development of AF post ablation are not well understood. To identify
patients undergoing CTI ablation for AFL most likely to develop AF. We
identified 114 patients without history of AF who underwent CTI AFL
ablation. We evaluated baseline characteristics, electrophysiology study
(EPS) data and echocardiographic data for incidence of AF within 3
years. Incident AF was identified in 46 patients (40%) during 600 + 405
days follow-up. Left atrial volume index (LAVI) was significantly
greater in patients who developed AF compared to those that did not (37
 12.2 ml/m2 vs 30  13.4 ml/m2, p=.004). EPS data was similar between
groups. Area under the receiver operator characteristic curve based on
the LAVI for prediction of AF was 0.7 (p = 0.004). Kaplan-Meier
estimated incidence of AF was significantly greater in patients with
LAVI ≥ 30 ml/m2 than LAVI < 30 ml/m2 (66% vs 27%, p=0.004).
Risk of incident AF in patients with LAVI > 40 mL/m2 was
similar to that of LAVI 30-40 ml/m2 (67% vs 63%, respectively,
p=0.97). In multivariate analysis LAVI remained the sole independent
predictor of incidence AF after CTI AFL ablation. LAVI ≥ 30 ml/m2 is
associated with significantly increased risk of incident AF following
CTI ablation for typical AFL. The utility of elevated LAVI for
identifying patients that may benefit from intensified arrhythmia
monitoring, or prophylactic AF ablation requires further evaluation.
Influence of Chronic Rhino sinusitis on Quality of Life and Risk of Depression and Lost Productivity: Population Based Survey
Authors: Alharbi Fahd , Ahmed Mohamed , Hakami Ayman , Hakami Khalid , Alhazmi Khalid , Alhazmi Alwaleed , Daghriri Hasan
Published: March 2022

Abstract Objectives; to evaluate the quality of life among patients with
chronic rhino sinusitis and the risk of developing depression. Design;
An observation cross section study. Setting; the quality of life was
assessed using 2-item sinonasal outcome test (SNOT-22) while Patient
Health Questionnaire-2 (PHQ-2) used for screen of depression and Stress
was analyzed using the Kessler Psychological Distress Scale (10 items
representing five psychological distress traits were assessed with a
five-point scale) Participants; 1072 patients with chronic rhino
sinusitis. Main outcome measures; Influence of Chronic Rhino sinusitis
on Quality of Life and Risk of Depression and Lost Productivity.
Results; the level of quality of life affection was moderate in 159
(24.77%) and severe in 483 (75.23%) of participants with CRS. The mean
score of PHQ-2 for assessing the risk of major depression was 4.17 +
1.74 in participants with CRS. 135 (21.03%) were normal (not at risk of
major depression) and 507 (78.97%) were at risk of major depression.
According to the Kessler Psychological Distress Scale: 759 patients
(70.8%) were positive with a mean total perceived stress score of 16.92
± 7.34, whereas 313(29.2%) were negative with a mean total perceived
stress score of 6.08 ± 2.7. The difference was statistically significant
Conclusions; Patients with CRS are at risk of depression, poor quality
of life and bad work performance. Despite the supportive treatment that
used to provide to such patients, a clinical comprehensive approach
should be considered to relief the impact on patients’ quality of life.
Diagnostic SARS-CoV-2 Cycle Threshold Value Predicts Disease Severity, Survival and 6-month Sequelae in COVID-19 Symptomatic Patients
Authors: Trunfio Mattia , Venuti Francesco , Alladio Francesca , Longo Bianca Maria , Burdino Elisa , Cerutti Francesco , Ghisetti Valeria , Bertucci Roberto , Picco Carlo , Bonora Stefano , Perri Giovanni Di , Calcagno Andrea
Published: March 2022

Background: No pathogen-specific prognostic biomarkers are yet available
for SARS-CoV-2. We sought to assess whether SARS-CoV-2 cycle threshold
value (Ct) at diagnosis may predict COVID-19 severity, clinical
manifestations and 6-month sequelae. Methods: Hospitalised and
outpatient cases were randomly sampled from the diagnoses of March and
data collected after 6 months by interview and from the regional
database for COVID-19 emergency. Patients were stratified according to
their RNA-dependent-RNA-polymerase Ct in the nasal-pharyngeal swab at
diagnosis: group A≤20.0, 20.028.0. Disease severity was classified
according to a composite scale evaluating hospital admission, worst
oxygen support required and survival. Results: One-hundred sixty-eight
survivors and thirty-two deceased patients were included: 27.5% in A
and B both, 45.0% in C. 90% of patients were symptomatic and 63.7%
were hospitalised. Median time from COVID-19 onset to swab collection
was 5 days. Lethality, number of comorbidities, disease severity, type
and amount of signs and symptoms, as well as 6-month sequelae inversely
distributed among the groups with respect to SARS-CoV-2 Ct. After
adjusting for confounding, SARS-CoV-2 Ct at diagnosis was still
associated with COVID-19-related death (p=0.023), disease severity
(p=0.023), amount of signs and symptoms (p<0.01) and presence
of sequelae (p<0.01). Conclusions: Early quantification of
SARS-CoV-2 along the course of the disease may be a useful predictive
marker to inform differential strategies of clinical management and
resource allocation.
Coloration, toxicity, and vertical distribution in larval phyllomedusine frogs: is there an anti-predator syndrome?
Authors: Pinto Diana , Amézquita Adolfo
Published: March 2022

Visually aposematic prey warns their potential predators about their
noxious condition by exhibiting conspicuous coloration. The larvae of
some phyllomedusine frogs bear bright patches on the body or tail that
suggest an anti-predator role, yet the experimental evidence of
aposematism in anuran larvae is notoriously scarce. Here, we assessed
and compared the conspicuousness and skin toxicity of three species of
phyllomedusine larvae which differ in coloration: Phyllomedusa
vaillanti, P. bicolor, and Callimedusa tomopterna. The conspicuous
colorations found on the dorsal area of the larvae could be directed to
aerial predators, therefore we evaluated the distribution of the larvae
in the depth of the water column. We also experimentally assessed their
palatability to dragonfly naiads as model predators. Additionally, we
observed that all these traits that make up the antipredatory syndrome
are modified along the ontogeny of the larvae. For this reason, we
assessed body size as another trait that conforms to the syndrome. Our
results support an antipredatory syndrome in larvae of P. vaillanti and
to a lesser degree in P. bicolor. The most conspicuous larvae were
indeed the most toxic. Moreover, these larvae were attacked less often
by predators which, in turn, died within a few hours. In both species,
the larvae remained close to the water column surface longer to
advertise their noxious condition towards predators. After we included
body size in the anti-predator syndrome, we observed that only the P.
vaillanti larger larvae were more conspicuous. Toxicity and palatability
were not related to body size. Nevertheless, vertical distribution
varied ontogenetically in P. bicolor. The largest larvae remained close
to the water surface most of the time, while the small ones were
distributed evenly throughout the water column.
On some mapping properties of positive operators
Authors: Amar Bougoutaia , Belacel Amar
Published: March 2022

In this paper, we give and study the notion of positive Cohen p-nuclear
operators. We prove a natural analog of the Pietsch domination theorem
for these classes, we characterize their conjugates and we valorize some
relationship between positive p-summing operators and positive strongly
q-summing using (Contemp. Math. 328,145-149(2003)).
Investigating the association between allergic diseases and COVID-19 in 400 Iranian patients
Authors: Darabi AmirHosein , Keshmiri Saeed , Joazan Sara , Tahmasebi Rahim , Movahed Ali , Zamani Marjan , Farrokhi Shokrollah
Published: March 2022

Background: Allergic diseases may play a role as a predisposing factor
for coronavirus disease 2019 (COVID-19). The aim of this study was to
investigate the allergy comorbidity and their association with the
patients with COVID-19. Methods: Demographic data, clinical symptoms,
laboratory and radiologic findings and having underlying diseases of the
patients were resgistered. Allergic diseases were identified by using
the standard GA2LEN questionnaire. The severity of COVID-19 was assessed
by visual analog scale (VAS) and ICU admission. Results: Of 400 COVID-19
patients, 158 (39.5%) had allergic diseases. And, there was a reversely
association beween an having allergy comorbidity and the severity of
COVID-19 infection (P= 0.005, relative risk, 0.96; 95% CI, 0.77-1.19).
The frequency of asthma, allergic rhinitis (AR), chronic rhinosinusitis,
atopic dermatitis, chronic urticaria, and food or drug allergy was
7.3%, 16%, 1.8%, 5%, 10% and 13.3%, respectively. Importantly,
only AR was reversly associated with the severity of COVID-19 (P= 0.02,
relative risk, 0.96; 95% CI, 0.77-1.19). Additionally, 43% of the
patients presented hypoxemia, and 93.5% had chest CT scan involvement.
Interestingly, patients with allergic than non allergic diseases had
significantly lower hypoxemia and chest CT involvement (P= 0.002 and P=
0.003, respectively). Conclusion: This study showed that allergic
diseases were not found to be a predisposing factor for the severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Importantly,
the patients with AR developed mild symptoms of COVID-19 and they were
admitted to ICU significantly less than the non-AR patients.
Ivermectin use associated with reduced duration of COVID-19 febrile illness in a community setting
Authors: Afsar Nasir , Ishaq Muhammad , Abbas Muslim , Mukarram Muhammad , Peracha Muhammad , Ishaq Khizra
Published: March 2022

AIMS: SARS-CoV-2 infection (COVID-19) is a potentially lethal disease
that may progress into severe respiratory distress syndrome requiring
ventilatory support. While azithromycin (AZI) and hydroxychloroquine
(HCQ) are considered similar to placebo in COVID-19, other drugs such as
ivermectin (IVER), are being repurposed to treat this pandemic. This
study was designed to assess the effects of ivermectin on duration of
febrile illness and disease outcomes in mild-to-moderate COVID-19
infection in a community setting. METHODS: In this case-control study 95
suspected patients of mild-to-moderate COVID-19 were included. The
controls (Group-A) received AZI+HCQ for seven days while the cases
(Group-B) received IVER+AZI+HCQ for six days. RESULTS: A total of 41
patients were in Group-B, while 54 patients were in Group-A. Group-B had
consistently and significantly shorter span of fever on days 5, 7, 10
and 14, where the logistic regression showed IVER as the major (Exp B
49•55; p<0•001) underlying factor. The Kaplan-Meier survival
analysis showed that Group-A had a prolonged febrile illness
(p<0•001). CONCLUSIONS: Ivermectin use is associated with
reduced duration of febrile illness in COVID-19 in outpatient setting,
thus potentially saving precious lives, reducing direct load on
healthcare facilities and preventing high cost of management in a
community setting.
Paediatric acute myeloid leukaemia: analysis by fluorescence in situ hybridisation
Authors: Chandrashekar Vani , Tarigopula Anil , Govindasami Perumal
Published: March 2022

Background: Paediatric acute myeloid leukaemia (AML) is heterogeneous.
Frequency of cytogenetic abnormalities varies from adults. Methods:
Children with de novo AML were included. Peripheral blood was analysed
for complete blood counts and bone marrow was analysed by fluorescence
in situ hybridisation for genetic abnormalities. Results: 53.6%
patients had cytogenetic abnormalities. Recurrent genetic abnormalities
were seen in 34.7%. Commonest recurrent genetic abnormality was
RUNX1-RUNX1T1 rearrangement seen in 14.4%, followed by PML-RARA
rearrangement seen in 8.6%, MLL gene rearrangement in 8.6% and
CBFB-MYH11 rearrangement in 2.8% patients. In children aged more than
five years, PML-RARA and RUNX1-RUNX1T1 were commonest whereas, in
children aged five and less, RUNX1-RUNX1T1 and MLL rearrangements were
the only recurrent genetic abnormalities. Patients with cytogenetic
abnormalities differed significantly with respect to hemoglobin, total
leucocyte count and platelet count. Conclusion: FISH alone can classify
patients into AML with common recurrent genetic abnormalities. However,
other methods are required for complete classification.
Therapeutic and prophylactic effect of the first experimental bacteriophage treatment to control diarrhoea caused by E. coli in newborn calves
Authors: Alomari Mohammed , Dec Marta , Nowaczek Anna , Puchalski Andrzej , Wernicki Andrzej , Kowalski Cezary , Urban-Chmiel Renata
Published: March 2022

The prevalence of antibiotic resistant bacteria causing neonatal
diarrhoea in calves has become a serious problem in the control of
infection. Due to increasing antibiotic resistance, the bacteriophages
with probiotics are considered the best alternative. The aim of study
was to evaluate the using a suppository with probiotic strains of
Lactobacillus spp. and bacteriophages specific for pathogenic E. coli in
young calves with diarrhoea. The study evaluated therapeutic and
prophylactic effects. The study was carried out on 24 female HF calves,
aged 2 to 7 days and weighing from 35-46 kg. The calves were divided
into four groups (n=6) each: Group 1 – healthy control, received no
medicine; Group 2 – positive control with diarrhoea; Group 3 – healthy
calves received medicine; Group 4 – calves with diarrhoea received
medicine.The animals received suppositories with Lactobacillus spp. and
bacteriophages specific for pathogenic E. coli for five days. On the
first day, calves received the suppositories twice– in the morning and
12 hours later; subsequently they were administered once a day. The
health status of calves was observed for 11d after the first application
of suppositories. A protective and preventive effect of the experimental
therapy was obtained in the research. The probiotic-phage suppositories
reduced the duration of diarrhoea in calves, completely eliminating it
within 24-48 hours after application The used therapy stimulated the
activation of immune mechanisms in calves, which translated into an
enhanced specific and non-specific response and increased resistance to
infection.
Maternal anemia and long-term respiratory morbidity of the offspring -- Results of a population-based cohort
Authors: Alcalay Idan , Sheiner Eyal , Wainstoc Tamar
Published: March 2022

Objective: To evaluate the association between prenatal maternal anemia
(hemoglobin, Hb<11 g/dl) and long-term respiratory morbidity
of the offspring. Methods: A retrospective population-based cohort
analysis was performed at the Soroka University Medical Center (SUMC), a
single regional tertiary medical center. All singletons born between the
years 1991-2014 and discharged alive were included in the study.
Offspring with congenital malformations were excluded. The three study
groups were defined based on maternal Hb levels, measured upon
postpartum discharge: <7.0 (severe anemia), 7.0-11.0 (mild to
moderate anemia), and ≥11.0 g/dl (unexposed). Respiratory morbidity
diagnosis was based on predefined ICD-9 codes of lung disease
definitions, based on the medical records of the hospitalized offspring
in SUMC. A Kaplan-Meier survival curve was formed to compare the
cumulative respiratory morbidity during the study follow-up period
between the study groups, and a multivariable Cox survival analysis was
used to control for cofounders. Results: During the study period,
214,305 deliveries met the inclusion criteria. Of those, 807 (0.3%)
mothers had severe anemia, 105,196 (49.1%) mothers had mild-moderate
anemia, and the remaining were not anemic (108,302, 50.5%). Respiratory
hospitalization incidence was found to be significantly higher among the
offspring born to anemic mothers (6.2%; 5.3% and 5.1%; p = 0.020, p
for trend=0.007 in severe, mild-to-moderate and non-anemic mothers,
respectively). The association between maternal anemia and
respiratory-related hospitalization of the offspring remained
significant among the mild-moderate anemic group after adjusting for
confounders in the Cox proportional hazards model (adjusted hazard
ratio=1.1; 95% CI 1.05-1.14; p < 0.01). As to the association
between severe maternal anemia and offspring morbidity no significant
differences were found (p= 0.133), although a linear hazard
dose-response ratio was found between the mild-moderate and severe
anemic groups (aHR=1.09, aHR 1.24, respectively). Conclusion: Maternal
anemia was found to be associated with long-term respiratory morbidity
of the offspring.
TpIRT1 is a transition metal transporter in Polish Wheat (Triticum polonicum L.) with a broad substrate specificity
Authors: Jiang Yulin , Chen Xing , Chai Songyue , Sheng Huajin , Sha Lina , Fan Xing , Zeng Jian , Kang Houyang , Zhang Haiqin , Xiao Xue , Zhou Yonghong , Vatamaniuk Olena , Wang Yi
Published: March 2022

Uptake and internal transport of micronutrients are essential for plant
growth, development, and yield. In this regard, Iron Regulated
Transporters (IRTs) from the ZIP (Zinc-regulated, iron-regulated
transporter-like proteins) family play an important role in transition
metal uptake. The majority of studies have been focused on IRT1-like
proteins in diploid species, but few in polyploids. Here, we studied the
function of TpIRT1A and TpIRT1B homoeologs in a tetraploid crop, Polish
wheat (Triticum polonicum). Our results highlighted the importance of
TpIRT1 in the uptake and translocation of Fe, Mn, Co, and Cd with direct
implications for wheat yield potential. Both TpIRT1A and TpIRT1B were
located at the plasma membrane and internal vesicles in Arabidopsis
protoplasts, and responsible for Cd and Co sensitivity in yeast. The
over-expression of TpIRT1B in A. thaliana increased Fe, Mn, Co, and Cd
concentration in its tissues and improved plant growth under Fe, Mn, and
Co deficiencies, while caused more sensitive to Cd than wild-type plant.
Functional analysis of IRT1 homoeologs from tetraploid and diploid
ancestral wheat species in yeast disclosed four distinct amino acid
residues in TdiIRT1B (T. dicoccum) and TtuIRT1B (T. turgidum). Together,
these results increase the knowledge of IRT1 function in a global crop,
wheat.
Epidemiologic Analysis of Pediatric Maxillofacial Trauma and Review of the Literature
Authors: Tabakan Ibrahim , Eser Cengiz , Gencel Eyuphan , Kokaçya Ömer
Published: March 2022

Background: The aim of the study is to make epidemiologic analysis of
pediatric maxillafacial traumas and discuss the mechanisms of injury,
etiology, treatment approaches and complications by comparing with adult
maxillofacial traumas. Method: Pediatric maxillofacial trauma patients
who presented to the Department of Plastic, Reconstructive and Aesthetic
Surgery at our hospital, for 5 years between 2014 and 2018 were
evaluated retrospectively. Age and gender distributions of the patients
were determined. Duration of hospitalization, etiology of trauma,
location of the fracture, and treatment methods applied were determined,
and the results of these treatments and complications that developed
were evaluated. Results: The fractures were the most common at the age
of 17 years (17.2%). The most common cause of trauma in patients
operated for maxillofacial trauma was fall (48.3%). Most operations
were performed in the age range of 12–18 years (49.4%), and panfacial
fractures were the most frequently operated fracture localization
(27.58%). Conclusion: The simplest and most effective treatment should
be applied for pediatric maxillofacial trauma. While conservative
treatments may be sufficient in minimally displaced fractures, open
reduction and internal fixation methods are applied in fractures with
greater displacement. Fixation materials must necessarily be removed
since they prevent bone growth. The use of bioabsorbable plates has been
limited in recent years due to their high costs.
Inotuzumab ozogamicin is an effective treatment for CD22-positive acute undifferentiated leukemia: A case report
Authors: Akazawa Ryo , Kato Itaru , Kubota Hirohito , Isobe Kiyotaka , Masuno Hiroaki , Mikami Masamitsu , Shiota Mitsutaka , Kozuki Kagehiro , Kawabata Naoko , Tanaka Kuniaki , Saida Satoshi , Umeda Katsutsugu , Hiramatsu Hidefumi , Adachi Souichi , Takita Junko
Published: March 2022

Acute undifferentiated leukemia (AUL) is a rare subtype of leukemia that
expresses no lineage-specific markers; no optimal treatment for AUL has
been established. Here, we report a 16-year-old female with
CD22-positive refractory AUL who responded well to inotuzumab ozogamicin
(InO). Minimal residual disease negativity was achieved using InO,
followed by HLA-mismatched unrelated bone marrow transplantation (BMT).
Although grade II veno-occlusive disease/sinusoidal obstruction syndrome
occurred, it improved immediately. She remained disease-free at 10
months post-BMT, without severe complications (grade III–IV). This case
demonstrates the feasibility of a treatment strategy using InO against
CD22-positive AUL.
Left Volume Atrial Index and P-Wave Dispersion as Predictors of Postoperative Atrial Fibrillation after Coronary Artery Bypass Graft: a Retrospective Cohort Study
Authors: Achmad Chaerul , Tiksnadi Badai , Akbar Mohammad , Karwiky Giky , Sihite Teddy , Pramudya Arsha , Iqbal Mohammad , Febrianora Mega
Published: March 2022

Background: Preoperative left atrial remodeling as Postoperative atrial
fibrillation (POAF) predisposing factors could be measured by left
atrial volume index (LAVI) and P-wave dispersion. Aim: This study aimed
to assess P-wave dispersion and LAVI as preoperative predictors of POAF
among patients who underwent Coronary Artery Bypass Graft (CABG).
Methods: An analytical retrospective cohort study was performed on
patients who underwent CABG. The P-wave dispersion and POAF were
evaluated based on documented ECG results. LAVI size was collected from
echocardiographic reports. Hazard ratios of P-wave dispersion and LAVI
for POAF were analyzed using Cox proportional hazard model. Result: A
total of 42 subjects (57±1 years) were included in this study. POAF
occurred in 28.6% of patients at a median of 2 days after CABG. P-wave
dispersion was significantly longer in patients in whom AF was developed
(53.03±3.82ms vs. 44.01±1.98ms, p:0.028), while LAVI difference was not
significant. The Cox proportional hazard model showed a significant
association between P-wave dispersion and risk of POAF (HR 1.05, CI95%,
1.001-1.103;p=0.048). There was no association between LAVI and risk of
POAF (HR1.003, CI 95%, 0.965-1.044;p=0.864). Conclusion: P-wave
dispersion is a predictor of POAF in patients who underwent CABG. Risk
stratification using P-wave dispersion enables clinicians to identify
high-risk patients before CABG surgery.
Acute coronary syndrome following cesarean section: a case report & review of literature.
Authors: Alwaheidi Dina , tbishat laith , Wani Lateef , Alqahtani Awad , alaasmi shabib , Khattak Masood
Published: March 2022

Abstract Spontaneous coronary artery dissection (SCAD) is considered one
of the rare causes of acute coronary syndrome. It’s been related to many
predisposing factors mostly connective tissue disorders, fibromuscular
dysplasia in particular, traumatic and atherosclerosis as the most
common etiologic factor. It occurs predominantly more in women & is
been associated with stress & pregnancy. Here we present a rare case of
coronary artery dissection extending to left main coronary artery in 33
years old women post elective cesarean section.
Uncovering the potential basis of the structural and functional glitches of VRQPrP
Authors: Teferedegn Eden Yitna , Ün Cemal
Published: March 2022

Altered physiologic function of prion is one of the proposed mechanisms
of neurodegeneration through perturbed activities of PrPC.Experimental
studies reported the link between sequence variation in the functional
domains of prion protein and scrapie disease development .This study
highlights how variants could potentially affect the prion protein
dynamics i.e. conformational flexibility, ligand binding affinity,
solvent accessibility and probability score of amyloid forming hotspot
sequences. Secondary structures were predicted using The MINNUS
server-POLYVIEW-2D.3D structure Residue specific ligand binding
probability and secondary structure were predicted using I-Tasser online
server.SDM prediction pipeline to assess the potential effect of
variants. Amyloidogenicity propensity of hotspot heptapeptides were
predicted using Multiple Instance Learning Based Amyloid Prediction
(MILAMP) online pipeline VRQ PrP diplayed broken helix flanked by strand
and it has has more strand percentage than ARR PrP.All hotspot sequences
containing the resistant variant at 136 and 171 codons were predicted to
have lower amyloid propensity.The ligand residue was predicted to be
buried in VRQ but exposed in ARR
Finite time blow-up for the heat flow of H-surface with constant mean curvature
Authors: Li Haixia
Published: March 2022

In this paper, the authors consider an initial boundary value problem
for the heat flow of equation of surfaces with constant mean curvatures,
which was investigated in [On the heat flow of equation of surfaces of
constant mean curvatures, Manuscripta Mathematica, 2011, 134: 259-271]
by Huang et al., where global well-posedness and finite time blow-up of
regular solutions were obtained. Their results are complemented in this
paper in the sense that some new conditions on the initial data are
provided for the solutions to develop finite time singularity.
Patient Centered Outcomes: Domain Importance Predicts Health Care Use following Physical Therapy
Authors: Zeppieri Giorgio , George Steven , Bialosky Joel , Lentz Trevor
Published: March 2022

Rationale, aims, objectives: Identifying patients with musculoskeletal
pain at risk for additional health care use following physical therapy
is important for improving the value proposition of physical therapists.
We previously identified 3 patient subgroups based on importance of
improvement in specific outcomes: 1) Multiple Outcome Domains Important
(MDI), 2) Pain and Function Outcomes Important (PFI), and 3) Pain
Important (PI). Outcome importance subgroup membership has not been
investigated as a potential predictor of future care use. The aim of
this study was to determine whether patient-determined outcome
importance subgroup membership predicts healthcare utilization
subsequent to an episode of physical therapy for musculoskeletal pain.
Methods: 246 patients seeking physical therapy that provided 12-month
outcome data in the Orthopedic Physical Therapy Investigative Network’s
(OPT-IN) Optimal Screening for Prediction of Referral and Outcome
(OSPRO) cohort. Demographics, health-related history, numeric pain
rating, region specific disability, and comorbidly were collected at
baseline. Outcome importance subgroup membership was assigned based on
responses to the Patient Centered Outcome Questionnaire (PCOQ). At 1
year, patients reported use of health care since discharge from physical
therapy. Separate logistic regression models determined whether outcome
importance subgroup membership predicted additional healthcare or use of
specific services. Results: The PI subgroup had higher adjusted odds
(95% CI) of at least one additional health care service [aOR = 2.40
(1.01-5.71)]; and when specific services were further evaluated there
were higher odds of opioid use [aOR = 11.25, (3.50-36.20)],
injection [aOR = 4.41, (1.41-13.79)] and surgery [aOR = 5.58,
(1.32-23.66)]. There were no differences between the PFI and MDI group
for odds of additional health care use or any specific services.
Conclusions: Patients with a singular focus on improvements in pain are
at higher risk for opioid use, injection, and surgery following an
episode of care with a physical therapist.
Rare Late Presentation of Severe Congenital Hemophilia A in a Ugandan Soldier - Case Report
Authors: Atwiine Barnabas , Airewele Gladstone
Published: March 2022

Patients with severe hemophilia are usually diagnosed in infancy due to
early onset of abnormal bleeding symptoms that characterize the disease.
Affected individuals require coagulation factor routinely or prior to
surgical procedures to prevent bleeding and are usually excluded from
activities with a high risk of trauma. A 25-year old active-duty soldier
was diagnosed with severe congenital hemophilia A after presenting with
initial episode of prolonged bleeding. His case demonstrates a rare late
presentation of a disease that typically presents in infancy and
highlights the need for improving knowledge about bleeding disorders
among health workers and the community.
Some parameterized quantum Simpson's and quantum Newton's integral inequalities via quantum differentiable convex mappings
Authors: You Xuexiao , Ali Muhammad Aamir , BUDAK Hüseyin , Zhao Dafang
Published: March 2022

In this work, two generalized quantum integral identities are proved by
using some parameters. By utilizing these equalities we present several
parameterized quantum inequalities for convex mappings. These quantum
inequalities generalize many of the important inequalities that exist in
the literature, such as quantum trapezoid inequalities, quantum
Simpson’s inequalities and quantum Newton’s inequalities. We also give
some new midpoint type inequalities as special cases. The results in
this work naturally generalize the results for the Riemann integral.