bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptome Analysis of Pistacia vera Inflorescence Buds in Bearing and Non-Bearing Shoots Reveals the Molecular Mechanism Causing Premature Flower Bud Abscission
Authors: Benny Jubina , Marra Francesco Paolo , Giovino Antonio , Balan Bipin , Caruso Tiziano , Martinelli Federico , Marchese Annalisa
Published: July 2020

The alteration of heavy (“ON/bearing”) and light (“OFF/non-bearing”) yield in pistachio (Pistacia vera L.) has been reported to result from the abscission of inflorescence buds on high yielding trees during the summer, but the regulatory mechanisms involved in this bud abscission remain unclear. The analysis provides insights into the transcript changes between inflorescence buds on bearing and non-bearing shoots, that we indicated as “ON” and “OFF”, and shed light on the molecular mechanisms causing premature inflorescence bud abscission in the pistachio cultivar “Bianca” which can be related to the alternate bearing behavior. In this study, a transcriptome analysis was performed in inflorescence buds of “ON” and “OFF” shoots. A total of 14,330 differentially expressed genes (DEGs), most of which are involved in sugar metabolism, plant hormone pathways, secondary metabolism and oxidative stress pathway, were identified. Our results shed light on the molecular mechanisms underlying inflorescence bud abscission in pistachio and we proposed a hypothetical model behind the molecular mechanism causing this abscission in “ON” shoots. Results highlighted how changes in genes expressed in nutrient pathways (carbohydrates and mineral elements) in pistachio “ON” vs. “OFF” inflorescence buds triggers a cascade of events involving trehalose-6-phosphate and target of rapamycin (TOR) signaling, SnRK1 complex, hormones, polyamines and ROS which end, through programmed cell death and autophagy phenomena, with the abscission of inflorescence buds. This is the first study reporting gene expression profiling of the fate of “ON” and “OFF” inflorescence buds associated with the alternate bearing in the pistachio.
Novel NDUFA13 Mutations Associated with OXPHOS Deficiency and Leigh Syndrome: A Second Family Report
Authors: González-Quintana Adrián , García-Consuegra Inés , Belanger-Quintana Amaya , Serrano-Lorenzo Pablo , Lucia Alejandro , Blázquez Alberto , Docampo Jorge , Ugalde Cristina , Morán María , Arenas Joaquín , Martín Miguel A.
Published: July 2020

Leigh syndrome (LS) usually presents as an early onset mitochondrial encephalopathy characterized by bilateral symmetric lesions in the basal ganglia and cerebral stem. More than 75 genes have been associated with this condition, including genes involved in the biogenesis of mitochondrial complex I (CI). In this study, we used a next-generation sequencing (NGS) panel to identify two novel biallelic variants in the NADH:ubiquinone oxidoreductase subunit A13 (NDUFA13) gene in a patient with isolated CI deficiency in skeletal muscle. Our patient, who represents the second family report with mutations in the CI NDUFA13 subunit, presented with LS lesions in brain magnetic resonance imaging, mild hypertrophic cardiomyopathy, and progressive spastic tetraparesis. This phenotype manifestation is different from that previously described in the first NDUFA13 family, which was predominantly characterized by neurosensorial symptoms. Both in silico pathogenicity predictions and oxidative phosphorylation (OXPHOS) functional findings in patient’s skin fibroblasts (delayed cell growth, isolated CI enzyme defect, decreased basal and maximal oxygen consumption and as well as ATP production, together with markedly diminished levels of the NDUFA13 protein, CI, and respirasomes) suggest that these novel variants in the NDUFA13 gene are the underlying cause of the CI defect, expanding the genetic heterogeneity of LS.
Literature Review of BARD1 as a Cancer Predisposing Gene with a Focus on Breast and Ovarian Cancers
Authors: Alenezi Wejdan M. , Fierheller Caitlin T. , Recio Neil , Tonin Patricia N.
Published: July 2020

Soon after the discovery of BRCA1 and BRCA2 over 20 years ago, it became apparent that not all hereditary breast and/or ovarian cancer syndrome families were explained by germline variants in these cancer predisposing genes, suggesting that other such genes have yet to be discovered. BRCA1-associated ring domain (BARD1), a direct interacting partner of BRCA1, was one of the earliest candidates investigated. Sequencing analyses revealed that potentially pathogenic BARD1 variants likely conferred a low–moderate risk to hereditary breast cancer, but this association is inconsistent. Here, we review studies of BARD1 as a cancer predisposing gene and illustrate the challenge of discovering additional cancer risk genes for hereditary breast and/or ovarian cancer. We selected peer reviewed research articles that focused on three themes: (i) sequence analyses of BARD1 to identify potentially pathogenic germline variants in adult hereditary cancer syndromes; (ii) biological assays of BARD1 variants to assess their effect on protein function; and (iii) association studies of BARD1 variants in family-based and case-control study groups to assess cancer risk. In conclusion, BARD1 is likely to be a low–moderate penetrance breast cancer risk gene.
Opportunities for Quality Improvement Programs (QIPs) in the Nutrition Support of Patients with Cancer
Authors: Arensberg Mary Beth , Richards Julie , Benjamin Jyoti , Kerr Kirk , Hegazi Refaat
Published: July 2020

Malnutrition in patients with cancer is a ubiquitous but neglected problem that can reduce patient survival/quality of life and increase treatment interruptions, readmission rates, and healthcare costs. Malnutrition interventions, including nutrition support through dietary counseling, diet fortification, oral nutrition supplements (ONS), and enteral and parenteral nutrition can help improve health outcomes. However, nutritional care standards and interventions for cancer are ambiguous and inconsistently applied. The lack of systematic malnutrition screening and intervention in ambulatory cancer care has especially significant consequences and thus the nutrition support of patients with cancer represents an area for quality improvement. United States healthcare payment models such as the Oncology Care Model are linked to quality of care and health outcomes. Quality improvement programs (QIPs) can advance patient-centered care, perfect care processes, and help healthcare professionals meet their quality measure performance goals. Malnutrition QIPs like the Malnutrition Quality Improvement Initiative (MQii) have been shown to be effective in identifying and treating malnutrition. However, little is known about or has been reported on nutrition or malnutrition-focused QIPs in cancer care. This paper provides information to support translational research on quality improvement and outlines the gaps and potential opportunities for QIPs in the nutrition support of patients with cancer.
Can Simple Tests Prior to Endoscopy Predict the OLGA Stage of Gastritis?
Authors: Bulbuloglu Ertan , Dagmura Hasan , Daldal Emin , Deresoy Alev , Bakir Huseyin , Ozsoy Ugur , Saglam Ali Ihsan , Demir Osman
Published: July 2020

Gastritis is a progressive disease that evolves from a non-atrophic to atrophic state and progresses through intestinal metaplasia, with some cases leading eventually to gastric cancer. Since gastritis by definition is an inflammatory process of the mucosal lining of the stomach and is usually associated with pain, we aimed to identify any association between the severity of gastritis and pain and a simple inflammatory marker derived from a complete blood count (CBC). This was a prospective cross-sectional study which enrolled 155 consecutive adult patients who underwent an upper endoscopy. Prior to the endoscopy, all patients were given a questionnaire, numerical rating scale (NRS) and complete blood count evaluation. The biopsy was obtained from the gastric mucosa according to the modified Sydney classification and scored with the Operative Link for Gastritis Assessment (OLGA) scoring system. The results showed a significant correlation between NRS and intestinal metaplasia (p < 0.01); moreover, a correlation was also found between the NRS and OLGA stage (r = 0.469, p < 0.001). A nonlinear curve was constructed for OLGA stage estimation according to NRS scores (r2 was found to be 0.442, with p < 0.001). The results also showed a correlation between the neutrophil to the lymphocyte ratio (NLR) and OLGA stage (p < 0.01). No correlation was found between the other gastric parameters and NLR (p > 0.05). Helicobacter pylori positivity did not correlate with NRS and NLR. As a conclusion, pain measured by NRS and NLR, which are simply calculated from the CBC prior to endoscopy, may be used to predict OLGA stages and estimate the severity of gastritis in endoscopy patients.
Estimation of Daily Spatial Snow Water Equivalent from Historical Snow Maps and Limited In-Situ Measurements
Authors: Malek Sami A. , Bales Roger C. , Glaser Steven D.
Published: July 2020

We present a scheme aimed at estimating daily spatial snow water equivalent (SWE) maps in real time and at high spatial resolution from scarce in-situ SWE measurements from Internet of Things (IoT) devices at actual sensor locations and historical SWE maps. The method consists of finding a background SWE field, followed by an update step using ensemble optimal interpolation to estimate the residuals. This novel approach allowed for areas with parsimonious sensors to have accurate estimates of spatial SWE without explicitly discovering and specifying the spatial-interpolation features. The scheme is evaluated across the Tuolumne River basin on a 50 m grid using an existing LiDAR-based product as the historical dataset. Results show a minimum RMSE of 30% at 50 m resolutions. Compared with the operational SNODAS product, reduction in error is up to 80% with historical LiDAR-measured snow depth as input data.
Moderating Role of Health Literacy on the Association between Alexithymia and Depressive Symptoms in Middle School Students
Authors: Song Xianbing , Li Danlin , Hu Jie , Yang Rong , Wan Yuhui , Fang Jun , Zhang Shichen
Published: July 2020

Depression is a common psychological problem in adolescents. At present, few studies have described the moderating role of health literacy on the association between alexithymia and depressive symptoms among adolescents. The purpose of this study was to explore the relation among health literacy, alexithymia, and depressive symptoms and the moderating role of health literacy in middle school students. In December 2017, data were collected from a school in Shenyang by the convenient sampling method using a questionnaire including demographic information, health literacy, alexithymia, and depressive symptoms. A total of 1068 junior and senior high school students were selected as subjects, and 1062 valid questionnaires were retained for analysis. Logistic regression models were used to examine the association between health literacy and alexithymia with depressive symptoms. The prevalence of depressive symptoms was 48.2%, and the prevalence of alexithymia was 17.9%. Low health literacy was significantly associated with depressive symptoms (odds ratio (OR) (95% confidence interval (CI)) = 3.648 (2.493–5.338)). Alexithymia was significantly correlated with depressive symptoms (OR (95% CI) = 3.091 (2.156–4.429)). Low health literacy was related to a greater increase in the risk of depressive symptoms for students with alexithymia (OR (95% CI) = 10.566 (5.175–21.570)). The findings suggest that alexithymia and health literacy are important factors influencing depressive symptoms and health literacy has a moderating role on the association between alexithymia and depressive symptoms. Enhancing health literacy of middle school students with alexithymia may improve their mental health.
How to Evaluate Provincial Ecological Civilization Construction? The Case of Jiangsu Province, China
Authors: Dong Feng , Pan Yuling , Zhang Xiaojie , Sun Ziyuan
Published: July 2020

Ecological civilization is a transcendence of industrial civilization. The improvement of China’s ecological civilization system can not only provide developing countries with an empirical reference for ecological civilization construction, but also make a huge contribution to the progress of global ecological civilization. This paper focuses on improving the provincial ecological civilization evaluation system. First, the Provincial Ecological Civilization Construction Evaluation Index System is established according to the Green Development Index System issued by the Chinese government in 2016, and then the applicability of the Spatio-Temporal Factor Analysis (STFA) is verified as the evaluation method of the provincial ecological civilization construction level. Further, taking Jiangsu Province, China as an example, this paper evaluates the level of ecological civilization construction in each city of Jiangsu Province, and analyzes the main factors affecting ecological civilization based on the evaluation results. Finally, according to the relationship between the ecological civilization construction and economic quality of each city, the four-quadrant method is applied to divide Jiangsu Province into four types of regions to help each city position itself in ecological civilization construction. This paper mainly draws the following conclusions: (1) Due to its simple operation and accurate evaluation results, STFA is applicable in evaluating the provincial ecological civilization construction level. (2) Taking Jiangsu Province as an example, it is found that the level of ecological civilization construction in the southwestern region is higher than that of the northeast coastal region in Jiangsu. Three inequality indices are applied to measure the regional inequality of ecological civilization construction among 13 cities, which is relatively high in recent years. (3) By analyzing the results of STFA, it is found that economic quality and natural protection are not only the main factors affecting the ecological civilization construction in Jiangsu province, but also the main reasons for the regional heterogeneity of provincial ecological civilization. (4) For the four types of regions, suggestions are proposed according to the characteristics of each region to help improve the provincial ecological civilization construction level. This paper not only improves China’s ecological civilization construction system, but also provides information for the provincial ecological civilization construction in developing countries.
Cinematographic Habits of Future Spanish Teachers from a Socio-Educational Perspective
Authors: Lorenzo-Lledó Alejandro , Lledó Asunción , Pérez-Vázquez Elena , Lorenzo Gonzalo
Published: July 2020

One of the key features of today′s society is the role of technology and mass-media. Among these tools, cinema has influenced successive generations more than 100 years. From an educational point of view, it is a resource of high pedagogical value. Moreover, it is present in the daily life of university students who will be the future teachers. Therefore, the aim of this study is to find out the film consumption habits of students in the teacher′s degree in Spanish universities. A quantitative approach was adopted with a survey design, and the national sample was made up of 4659 students. They ware from the different regions of Spain and 58 universities. The questionnaire called Percepciones sobre las potencialidades del cine como recurso didáctico en las aulas de Infantil y Primaria (PECID) was designed ad hoc for this research. The results obtained showed that most students have a weekly habit of consuming films, with fiction being the most popular type of film. Likewise, the viewing of films is preferably done through television and the computer. It can be concluded that this familiarity with cinema outside the university should be complemented with specific training in the teacher′s degree. In this sense, it contributes to an optimal application of cinema.
The Health Status of Informal Waste Collectors in Korea
Authors: Ahn Joonho , Lee Jaeyong , Park Hyeyeon , Kang Yangwon , Kang Chungwon , You Young-Jin , Kang Mo-Yeol
Published: July 2020

Background: A broad, holistic approach was performed among informal waste collectors (IWCs) in Korea to understand their complex multidimensional health and safety problems. Methods: In the quantitative study, a survey of IWCs was conducted at four junk shops in Gangbuk-gu, Seoul, and survey data were used to calculate age-standardized prevalence rates based on comparisons with the general population in Korea. A qualitative study was also performed to provide more details on IWCs’ occupational and musculoskeletal injuries and depression. Results: In the quantitative study, the age-standardized prevalence rate (aSPR) of occupational injury was higher than that of the general standard population (aSPR: 10.42, 95% confidence interval (CI) 5.19–18.64) and that of blue-collar workers (aSPR: 4.65, 95% CI 2.32–8.32). Regarding musculoskeletal problems, compared to employed populations, the aSPRs of shoulder pain (aSPR: 2.63, 95% CI 1.60–4.06), wrist pain (aSPR: 3.33, 95% CI 1.33–6.86), knee pain (aSPR: 1.51, 95% CI 1.01–2.17), and ankle pain (aSPR: 3.54, 95% CI 1.14–8.26) were higher. Regarding psychological problems, depression (aSPR: 2.55, 95% CI 1.27–4.56) and suicidal or self-harm ideation (aSPR: 2.09, 95% CI 1.11–3.58) were higher compared to general populations. Through the qualitative study and case study on muscular problems, more details on the work environment problems of IWCs were obtained. Conclusions: IWCs are exposed to various occupational hazards and lack proper protection. They show a high prevalence of occupational injury, musculoskeletal disease, and depression.
Problematic Relationships with Smartphones of Spanish and Colombian University Students
Authors: Marín-Díaz Verónica , Muñoz-González Juan Manuel , Sampedro-Requena Begoña-Esther
Published: July 2020

The presence of smartphones in the lives of the population in general, and of youth in particular, is evident, and is derived from elements such as the diversity of prices as well as the ease of access of all the resources that can be reached through the internet. With the use of a descriptive approach using a quantitative poll, the objective of the present study was to discover the opinions of university students in Spain and Colombia about smartphone use, as well as the consequences of its use, and if this use could derive into so-called problematic smartphone use (PSU). For gathering the information, the Mobile Phone Problematic Use Scale (MPPUSA) was utilized, with a sample size n = 4009. The main result reached was that the model obtained is structured around six factors that determined the elements in light of PSU. The initial conclusion found was that the model applied can be utilized with Colombian students, with young Spanish women and students in the macro area of Social Sciences, the ones who had problematic behavior with the devices, as compared to the Health Sciences students who did not have it.
Reduced Gray Matter Volume and Risk of Falls in Parkinson’s Disease with Dementia Patients: A Voxel-Based Morphometry Study
Authors: Cheng Kai-Lun , Lin Li-Han , Chen Po-Cheng , Chiang Pi-Ling , Chen Yueh-Sheng , Chen Hsiu-Ling , Chen Meng-Hsiang , Chou Kun-Hsien , Li Shau-Hsuan , Lu Cheng-Hsien , Lin Wei-Che
Published: July 2020

Purpose: Risk of falls is a common sequela affecting patients with Parkinson’s disease (PD). Although motor impairment and dementia are correlated with falls, associations of brain structure and cognition deficits with falls remain unclear. Material and Methods: Thirty-five PD patients with dementia (PDD), and 37 age- and sex-matched healthy subjects were recruited for this study. All participants received structural magnetic resonance imaging (MRI) scans, and disease severity and cognitive evaluations. Additionally, patient fall history was recorded. Regional structural differences between PDD with and without fall groups were performed using voxel-based morphometry processing. Stepwise logistic regression analysis was used to predict the fall risk in PDD patients. Results: The results revealed that 48% of PDD patients experienced falls. Significantly lower gray matter volume (GMV) in the left calcarine and right inferior frontal gyrus in PDD patients with fall compared to PDD patients without fall were noted. The PDD patients with fall exhibited worse UPDRS-II scores compared to PDD patients without fall and were negatively correlated with lower GMV in the left calcarine (p/r = 0.004/−0.492). Furthermore, lower GMV in the left calcarine and right inferior frontal gyrus correlated with poor attention and executive functional test scores. Multiple logistic regression analysis showed that the left calcarine was the only variable (p = 0.004, 95% CI = 0.00–0.00) negatively associated with the fall event. Conclusions: PDD patients exhibiting impaired motor function, lower GMV in the left calcarine and right inferior frontal gyrus, and notable cognitive deficits may have increased risk of falls.
Coffee Consumption, Genetic Polymorphisms, and the Risk of Type 2 Diabetes Mellitus: A Pooled Analysis of Four Prospective Cohort Studies
Authors: Kim An Na , Cho Hyun Jeong , Youn Jiyoung , Jin Taiyue , Kang Moonil , Sung Joohon , Lee Jung Eun
Published: July 2020

The association between coffee consumption and the risk of type 2 diabetes may vary by genetic variants. Our study addresses the question of whether the incidence of type 2 diabetes is related to the consumption of coffee and whether this relationship is modified by polymorphisms related to type 2 diabetes. We performed a pooled analysis of four Korean prospective studies that included 71,527 participants; median follow-up periods ranged between 2 and 13 years. All participants had completed a validated food-frequency questionnaire (FFQ) at baseline. The odds ratios (ORs) and 95% confidence intervals (CIs) for type 2 diabetes were calculated using logistic regression models. The ORs were combined using a fixed or random effects model depending on the heterogeneity across the studies. Compared with 0 to <0.5 cups/day of coffee consumption, the OR for type 2 diabetes was 0.89 (95% CI: 0.80–0.98, p for trend = 0.01) for ≥3 cups/day of coffee consumption. We did not observe significant interactions by five single nucleotide polymorphisms (SNPs) related to type 2 diabetes (CDKAL1 rs7756992, CDKN2A/B rs10811661, KCNJ11 rs5215, KCNQ1 rs163184, and PEPD rs3786897) in the association between coffee and the risk of type 2 diabetes. We found that coffee consumption was inversely associated with the risk of type 2 diabetes.
A Mini-Review of Ixodes Ticks Climate Sensitive Infection Dispersion Risk in the Nordic Region
Authors: van Oort Bob E. H. , Hovelsrud Grete K. , Risvoll Camilla , Mohr Christian W. , Jore Solveig
Published: July 2020

Climate change in the Nordic countries is projected to lead to both wetter and warmer seasons. This, in combination with associated vegetation changes and increased animal migration, increases the potential incidence of tick-borne diseases (TBD) where already occurring, and emergence in new places. At the same time, vegetation and animal management influence tick habitat and transmission risks. In this paper, we review the literature on Ixodes ricinus, the primary vector for TBD. Current and projected distribution changes and associated disease transmission risks are related to climate constraints and climate change, and this risk is discussed in the specific context of reindeer management. Our results indicate that climatic limitations for vectors and hosts, and environmental and societal/institutional conditions will have a significant role in determining the spreading of climate-sensitive infections (CSIs) under a changing climate. Management emerges as an important regulatory “tool” for tick and/or risk for disease transfer. In particular, shrub encroachment, and pasture and animal management, are important. The results underscore the need to take a seasonal view of TBD risks, such as (1) grazing and migratory (host) animal presence, (2) tick (vector) activity, (3) climate and vegetation, and (4) land and animal management, which all have seasonal cycles that may or may not coincide with different consequences of climate change on CSI migration. We conclude that risk management must be coordinated across the regions, and with other land-use management plans related to climate mitigation or food production to understand and address the changes in CSI risks.
The Impact of Parental Remote Migration and Parent-Child Relation Types on the Psychological Resilience of Rural Left-Behind Children in China
Authors: Liu Hongsheng , Liu Lige , Jin Xiaoyi
Published: July 2020

Using survey data of middle school students from Ye county in Henan province and Chenggu and Ningqiang county in Shaanxi province, China, adopting latent class analysis and hierarchical linear regression, this study analyzes the impact of parental remote migration and parent-child relation types on the psychological resilience of rural left-behind children. The results show that: Only mother’s remote migration has a significantly negative impact on the psychological resilience of rural left-behind children, the time of parental first migration, the distance of father’s migration, and children’s migration have no significant impacts; parent-child relation of “alienation connection and weak function” or parent-child relation combination of “parental alienation connection and weak function” is the most unfavorable factor for the psychological resilience of rural left-behind children, while father-child relation of “close connection but lacking function”, mother-child relation of “intimate connection and strong function”, and combination of “paternal close connection but lacking function - maternal intimate connection and strong function” are the most favorable factors. There is gender difference in the impact of father-child relation types and mother-child relation types, the impact of father-child relation types is stronger than that of mother-child relation types; harmonious parental relation, supportive friends, caring teachers, and moderate home-school interaction are favorable for the psychological resilience of rural left-behind children.
How Authenticity and Tradition Shift into Sustainability and Innovation: Evidence from Italian Agritourism
Authors: Palmi Pamela , Lezzi Greta Enrica
Published: July 2020

This study investigates the topic of innovation strategies based on tradition in the wake of sustainability, in the agritourism sector, as derived from the phenomenon of multifunctionality in agriculture. The results reveal that the tangible and intangible resources that originate from tradition are drivers for innovation. The research highlights how tradition, as grounded on diverse foundations, is able to generate novel products and services stemming from an innovative arrangement of past events, particularly the identity of a place, which brings out its authenticity and makes it even more attractive. In this paper, we delve into the multifaceted outcomes that tangible and intangible traditions have on the innovation and distinctive standing of this rising accommodation offer with regard to post-productivism agriculture, and how this is accomplished while also looking for sustainability. The sourced dataset is based on a qualitative investigation of 10 cases in the Salento area of Puglia, a region of southern Italy. Tradition-grounded strategies proved to have several viable routes leading to innovation and bear positive impacts on the territory and the creation of value, yielding significant results both for scholars and practitioners.
Chondroitin Sulfate Prevents STZ Induced Diabetic Osteoporosis through Decreasing Blood Glucose, AntiOxidative Stress, Anti-Inflammation and OPG/RANKL Expression Regulation
Authors: Zheng Hong Xing , Chen De Jing , Zu Yue Xin , Wang En Zhu , Qi Shan Shan
Published: July 2020

Chondroitin sulfate (CS) has antioxidative, anti-inflammatory, anti-osteoarthritic and hypoglycemic effects. However, whether it has antidiabetic osteoporosis effects has not been reported. Therefore, in this study, we established a STZ-induced diabetic rat model; CS (500 mg kg−1 d−1) was orally administrated for eight weeks to study its preventive effects on diabetic osteoporosis. The results showed that eight weeks of CS treatment improved the symptoms of diabetes; the CS-treated group has increased body weight, decreased water or food intake, decreased blood glucose, increased bone-mineral density, repaired bone morphology and decreased femoral osteoclasts and tibia adipocytes numbers. After CS treatment, bone histomorphometric parameters returned to normal, the levels of serum inflammatory cytokines (IL-1β, IL-6 and TNF-α) decreased significantly, serum SOD, GPX and CAT activities increased and MDA level increased. In the CS-treated group, the levels of serum ALP, CTX-1, TRACP 5b, osteocalcin and RANKL decreased and the serum RUNX 2 and OPG levels increased. Bone immunohistochemistry results showed that CS can effectively increase the expression of OPG and RUNX2 and reduce the expression of RANKL in diabetic rats. All of these indicate that CS could prevent STZ induced diabetic osteoporosis—mainly through decreasing blood glucose, antioxidative stress, anti-inflammation and regulation of OPG/RANKL expression. CS can therefore effectively prevent bone loss caused by diabetes.
Gangliosides and Neuroblastomas
Authors: Schengrund Cara-Lynne
Published: July 2020

The focus of this review is the ganglio-series of glycosphingolipids found in neuroblastoma (NB) and the myriad of unanswered questions associated with their possible role(s) in this cancer. NB is one of the more common solid malignancies of children. Five-year survival for those diagnosed with low risk NB is 90–95%, while that for children with high-risk NB is around 40–50%. Much of the survival rate reflects age of diagnosis with children under a year having a much better prognosis than those over two. Identification of expression of GD2 on the surface of most NB cells led to studies of the effectiveness and subsequent approval of anti-GD2 antibodies as a treatment modality. Despite much success, a subset of patients, possibly those whose tumors fail to express concentrations of gangliosides such as GD1b and GT1b found in tumors from patients with a good prognosis, have tumors refractory to treatment. These observations support discussion of what is known about control of ganglioside synthesis, and their actual functions in NB, as well as their possible relationship to treatment response.
Concomitant Infection of Helicobacter pylori and Intestinal Parasites in Adults Attending a Referral Centre for Parasitic Infections in North Eastern Italy
Authors: Pomari Elena , Ursini Tamara , Silva Ronaldo , Leonardi Martina , Ligozzi Marco , Angheben Andrea
Published: July 2020

Background: Helicobacter pylori and intestinal parasites are estimated to infect with high burden worldwide. However, their concomitant infections are poorly determined in industrialized countries, such as Italy. In this study we aim at describing the presence of H. pylori as well as the proportion of coinfections with intestinal parasites among subjects who attended a referral center for tropical diseases in Northern Italy. Methods: This was a case-control study. Screening for H. pylori and parasites was performed on stool samples of 93 adults from different geographical origin (Africa, Asia, South-America, East-Europe and Italy). H. pylori infection was examined by CLIA and its cagA positivity was determined by rtPCR. Intestinal parasites (i.e., protozoa and helminths) were examined by microscopy and rtPCR. Results: Sixty-one out of 93 patients (66%) were positive to H. pylori and 31 (33%) were cagA+. Among H. pylori positives, 45 (74%) had a concomitant infection. The coinfection H. pylori–Blastocystis was the most frequent one, followed by H. pylori–E. coli. Multivariable logistic regression showed that positivity to H. pylori was associated with having a coinfection. Conclusion: Our data suggested that H. pylori and intestinal parasitic infections are fairly common in subjects who attended a referral center for tropical diseases in Northern Italy. The high rate of H. pylori infection, and especially the positivity to the virulent cagA+, should be taken into consideration in subjects undergoing screening for parasitic infections.
Head-to-Head Accuracy Comparison of Three Commercial COVID-19 IgM/IgG Serology Rapid Tests
Authors: Andrey Diego O. , Cohen Patrick , Meyer Benjamin , Torriani Giulia , Yerly Sabine , Mazza Lena , Calame Adrien , Arm-Vernez Isabelle , Guessous Idris , Stringhini Silvia , Roux-Lombard Pascale , Fontao Lionel , Agoritsas Thomas , Stirnemann Jerôme , Reny Jean-Luc , Siegrist Claire-Anne , Eckerle Isabella , Kaiser Laurent , Vuilleumier Nicolas
Published: July 2020

Background: Comparative data of SARS-CoV-2 IgM/IgG serology rapid diagnostic tests (RDTs) is scarce. We thus performed a head-to-head comparison of three RDTs. Methods: In this unmatched case-control study, blood samples from 41 RT-PCR-confirmed COVID-19 cases and 50 negative controls were studied. The diagnostic accuracy of three commercially available COVID-19 RDTs: NTBIO (RDT-A), Orient-Gene (RDT-B), and MEDsan (RDT-C), against both a recombinant spike-expressing immunofluorescence assay (rIFA) and Euroimmun IgG ELISA, was assessed. RDT results concordant with the reference methods, and between whole blood and plasma, were established by the Kendall coefficient. Results: COVID-19 cases’ median time from RT-PCR to serology was 22 days (interquartile range (IQR) 13–31 days). Whole-blood IgG detection with RDT-A, -B, and -C showed 0.93, 0.83, and 0.98 concordance with rIFA. Against rIFA, RDT-A sensitivity (SN) was 92% (95% CI: 78–98) and specificity (SP) 100% (95% CI: 91–100), RDT-B showed 87% SN (95% CI: 72–95) and 98% SP (95% CI: 88–100), and RDT-C 100% SN (95% CI: 88–100) and 98% SP (95% CI: 88–100). Against ELISA, SN and SP were above 90% for all three RDTs. Conclusions: RDT-A and RDT-C displayed IgG detection SN and SP above 90% in whole blood. These RDTs could be considered in the absence of routine diagnostic serology facilities.
Hemming with Pre-Bent Inner Sheet for Joining Ultra-High Strength Steel Sheets of Automobile Parts
Authors: Abe Yohei , Ijichi Wataru , Mori Ken-ichiro , Nakagawa Kazuma
Published: July 2020

In order to join two ultra-high strength steel sheets with low ductility for automobile parts, a joining process by hemming with a pre-bent inner sheet was developed. In this joining, the pre-bent inner sheet instead of the conventional flat inner sheet was used to relax the deformation concentration of the outer sheet. Although 780 MPa steel sheets were joined without the pre-bent inner sheet, a fracture in the outer sheet occurred in joining the 980 MPa sheets due to the low ductility of the sheets. The 980 MPa and 1180 MPa sheets were successfully joined by hemming with the pre-bent inner sheet. In this process, the deformation of the upper sheet was relaxed by contacting with the inner sheet, and then the strain on the outer surface reduced. Although softening around a weld nugget occurred by heating in the conventional welded joint, work-hardening occurred in the hemmed joint. The joint strength was investigated and then the peel strength of the hemmed sheets was about a half of the welded one. It was found that the hemming process with the pre-bent inner sheet was effective for joining ultra-high strength steel sheets with low ductility.
Proper Use of Technical Standards in Offshore Petroleum Industry
Authors: Brkić Dejan , Praks Pavel
Published: July 2020

Ships for drilling need to operate in the territorial waters of many different countries which can have different technical standards and procedures. For example, the European Union and European Economic Area EU/EEA product safety directives exclude from their scope drilling ships and related equipment onboard. On the other hand, the EU/EEA offshore safety directive requires the application of all the best technical standards that are used worldwide in the oil and gas industry. Consequently, it is not easy to select the most appropriate technical standards that increase the overall level of safety and environmental protection whilst avoiding the costs of additional certifications. We will show how some technical standards and procedures, which are recognized worldwide by the petroleum industry, can be accepted by various standardization bodies, and how they can fulfil the essential health and safety requirements of certain directives. Emphasis will be placed on the prevention of fire and explosion, on the safe use of equipment under pressure, and on the protection of personnel who work with machinery. Additionally considered is how the proper use of adequate procedures available at the time would have prevented three large scale offshore petroleum accidents: the Macondo Deepwater Horizon in the Gulf of Mexico in 2010; the Montara in the Timor Sea in 2009; the Piper Alpha in the North Sea in 1988.
Overtopping Metrics and Coastal Safety: A Case of Study from the Catalan Coast
Authors: Altomare Corrado , Gironella Xavi , Suzuki Tomohiro , Viccione Giacomo , Saponieri Alessandra
Published: July 2020

Design criteria for coastal defenses exposed to wave overtopping are usually assessed by mean overtopping discharges and maximum individual overtopping volumes. However, it is often difficult to give clear and precise limits of tolerable overtopping for all kinds of layouts. A few studies analyzed the relationship between wave overtopping flows and hazard levels for people on sea dikes, confirming that one single value of admissible mean discharge or individual overtopping volume is not a sufficient indicator of the hazard, but detailed characterization of flow velocities and depths is required. This work presents the results of an experimental campaign aiming at analyzing the validity of the safety limits and design criteria for overtopping discharge applied to an urbanized stretch of the Catalan coast, exposed to significant overtopping events every stormy season. The work compares different safety criteria for pedestrians. The results prove that the safety of pedestrians on a sea dike can be still guaranteed, even for overtopping volumes larger than 1,000 L/m. Sea storms characterized by deep-water wave height between 3.6 and 4.5 m lead to overtopping flow depth values larger than 1 m and flow velocities up to 20 m/s. However, pedestrian hazard is proved to be linked to the combination of overtopping flow velocity and flow depth rather than to single maximum values of one of these parameters. The use of stability curves to assess people’s stability under overtopping waves is therefore advised.
Using a Gender-Responsive Land Rights Framework to Assess Youth Land Rights in Rural Liberia
Authors: Louis Elizabeth , Mauto Tizai , Dodd My-Lan , Heidenrich Tasha , Dolo Peter , Urey Emmanuel
Published: July 2020

This article summarizes the evidence on youth land rights in Liberia from a literature review combined with primary research from two separate studies: (1) A qualitative assessment conducted as formative research to inform the design of the Land Rights and Sustainable Development (LRSD) project for Landesa and its partners’ community level interventions; and (2) a quantitative baseline survey of program beneficiaries as part of an evaluation of the LRSD project. The findings are presented using a Gender-Responsive Land Rights Framework that examines youth land rights through a gender lens. The evidence highlights that female and male youth in Liberia face significant but different barriers to long-term access to land, as well as to participation in decisions related to land. Our suggested recommendations offer insights for the implementation of Liberia’s recently passed Land Rights Act as well as for community-level interventions focused on increasing youth land tenure security in Liberia.
The Role of Long Period Stacking Ordered Phase in Dynamic Recrystallization of a Mg–Gd–Y–Zn–Zr Alloy during Multi-Directional Forging Process
Authors: Liu Huiling , Meng Yingze , Yu Huisheng , Xu Wenlong , Zhang Siyang , Jia Leichen , Wu Guoqin
Published: July 2020

The Mg–Gd–Y–Zn–Zr alloy containing a long period stacking ordered (LPSO) phase was subjected to multi-pass deformation by means of a multi-directional forging process, and the microstructure evolution and the influence of the LPSO phase on its dynamic recrystallization (DRX) were studied. The results showed that multi-directional forging can effectively refine the grain with the DRX fraction increased, and DRXed grains lead to the decrease of the texture intensity, which can significantly improve the mechanical properties of the alloy. The different morphologies of the LPSO phase have different degrees of promotion relative to DRX behavior. The lamellar LPSO phase with kinks promoted dislocation plugging, where there could be a potential nucleation site for DRX grains. The fragmented lamellar LPSO phase promoted the DRX process through the particle-stimulated nucleation mechanism, and the block-shaped phase was more prone to stress concentration, which promoted DRX. These effects resulted in continuous grain refinement and a more uniform microstructure.
Design of Zeolite-Covalent Organic Frameworks for Methane Storage
Authors: Do Ha Huu , Kim Soo Young , Le Quyet Van , Pham-Tran Nguyen-Nguyen
Published: July 2020

A new type of zeolite-based covalent organic frameworks (ZCOFs) was designed under different topologies and linkers. In this study, the silicon atoms in zeolite structures were replaced by carbon atoms in thiophene, furan, and pyrrole linkers. Through the adoption of this strategy, 300 ZCOFs structures were constructed and simulated. Overall, the specific surface area of ZCOFs is in the range of 300–3500 m2/g, whereas the pore size is distributed from 3 to 27 Å. Furthermore, the pore volume exhibits a wide range between 0.01 and 1.5 cm3/g. Screening 300 ZCOFs with the criteria towards methane storage, 11 preliminary structures were selected. In addition, the Grand Canonical Monte Carlo technique was utilized to evaluate the CH4 adsorption ability of ZCOFs in a pressure ranging from 1 to 85 bar at a temperature of 298 K. The result reveals that two ZCOF structures: JST-S 183 v/v (65–5.8 bar) and NPT-S 177 v/v (35–1 bar) are considered as potential adsorbents for methane storage. Furthermore, the thermodynamic stability of representative structures is also checked base on quantum mechanical calculations.
Corrosion Behavior of AISI 1018 Carbon Steel in Localized Repairs of Mortars with Alkaline Cements and Engineered Cementitious Composites
Authors: Maldonado-Bandala Erick , Higueredo-Moctezuma Noema , Nieves-Mendoza Demetrio , Gaona-Tiburcio Citlalli , Zambrano-Robledo Patricia , Hernández-Martínez Héctor , Almeraya-Calderón Facundo
Published: July 2020

The selection of materials for repairs of reinforced concrete structures is a serious concern. They are chosen for the mechanical capacity that the repair mortar achieves. However, several important characteristics have been left aside, such as the adhesion of the repair mortar with the concrete substrate, the electrical resistivity and—hugely important—the protection against corrosion that the repair material can provide to the reinforcing steel. The aim of this work was to study the corrosion behavior of AISI 1018 carbon steel (CS) in mortars manufactured with alkaline cements, engineered cementitious composites (ECC), and supplementary cementitious materials (SCM). Two types of ordinary Portland cement (OPC) 30R and 40R were used. The constituent materials for the mortars with ECC mixture mortars they use OPC 40R, class F fly ash (FA), silica fume (SF) and polypropylene (PP) fibers. The sodium hydroxide (NaOH) and sodium silicate (Na2SiO3) were used as activating agents in alkali activated cements. The reinforced specimens were immersed in two different electrolytes, exposed to a 3.5 wt % of NaCl and Na2SO4 solutions, for 12 months and their electrochemical behavior was studied by half-cell potential (Ecorr) and linear polarization resistance (LPR) according to ASTM C876-15 and ASTM G59-97, respectively. The results obtained indicated that, the mortar they have the best performance and durability, is the conventional MCXF mortar, with OPC 30R and addition of 1% polypropylene PP fiber improves the behavior against the attack of chlorides and sulfates.
Enhanced Ibuprofen Adsorption and Desorption on Synthesized Functionalized Magnetic Multiwall Carbon Nanotubes from Aqueous Solution
Authors: Hanbali Ghadir , Jodeh Shehdeh , Hamed Othman , Bol Roland , Khalaf Bayan , Qdemat Asma , Samhan Subhi
Published: July 2020

In recent years, concerns have been raised about the occurrence of active raw materials and pharmaceutical ingredients that may be present in water, including wastewater, in the pharmaceutical industry. Wastewater treatment methods are not enough to completely remove active pharmaceuticals and other waste; thus, this study aims to assess the use of a multiwall carbon nanotube after derivatization and magnetization as a new and renewable absorbent for removing ibuprofen from an aqueous medium. The adsorbents were prepared by first oxidizing a multiwall carbon nanotube and then deriving the oxidized product with hydroxyl amine (m-MWCNT–HA), hydrazine (m-MWCNT–HYD), and amino acid (m-MWCNT–CYS). Adsorbents were characterized by Raman spectroscopy, Fourier Transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM and TEM), Brunauer–Emmett–Teller surface area analysis (BET), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Batch adsorption studies were conducted to study the effects of pH, temperature, time, and initial concentration of the adsorbate. Adsorption isotherm, kinetics, and thermodynamics studies were also conducted. The results show that the optimal pH for nearly complete removal of Ibu in a short time at room temperature was 4 for three adsorbents. The adsorption followed the Langmuir isotherm model with pseudo-second-order kinetics. The percentage of removal of ibuprofen reached up to 98.4%, 93%, and 61.5% for m-MWCNT–CYS, m-MWCNT–HYD, and m-MWCNT–HA respectively. To the best of our knowledge, the grafted MWCNTs presented in this work comprise the first example in the literature of oxidized MWCNT modified with such functionalities and applied for ibuprofen removal.
A Novel Algorithm with Multiple Consumer Demand Response Priorities in Residential Unbalanced LV Electricity Distribution Networks
Authors: Ivanov Ovidiu , Chattopadhyay Samiran , Banerjee Soumya , Neagu Bogdan-Constantin , Grigoras Gheorghe , Gavrilas Mihai
Published: July 2020

Demand Side Management (DSM) is becoming necessary in residential electricity distribution networks where local electricity trading is implemented. Amongst the DSM tools, Demand Response (DR) is used to engage the consumers in the market by voluntary disconnection of high consumption receptors at peak demand hours. As a part of the transition to Smart Grids, there is a high interest in DR applications for residential consumers connected in intelligent grids which allow remote controlling of receptors by electricity distribution system operators and Home Energy Management Systems (HEMS) at consumer homes. This paper proposes a novel algorithm for multi-objective DR optimization in low voltage distribution networks with unbalanced loads, that takes into account individual consumer comfort settings and several technical objectives for the network operator. Phase load balancing, two approaches for minimum comfort disturbance of consumers and two alternatives for network loss reduction are proposed as objectives for DR. An original and faster method of replacing load flow calculations in the evaluation of the feasible solutions is proposed. A case study demonstrates the capabilities of the algorithm.