bluuebunny/crossref_metadata_2025 huggingface dataset:
CLN8 Gene Compound Heterozygous Variants: A New Case and Protein Bioinformatics Analyses
Authors: Sharkia Rajech , Zalan Abdelnaser , Zahalka Hazar , Kessel Amit , Asaly Ayman , Al-Shareef Wasif , Mahajnah Muhammad
Published: August 2022

The CLN8 disease type refers to one of the neuronal ceroid lipofuscinoses (NCLs) which are the most common group of neurodegenerative diseases in childhood. The clinical phenotypes of this disease are progressive neurological deterioration that could lead to seizures, dementia, ataxia, visual failure, and various forms of abnormal movement. In the current study, we describe two patients who presented with atypical phenotypic manifestation and protracted clinical course of CLN8 carrying a novel compound heterozygous variant at the CLN8 gene. Our patients developed a mild phenotype of CLN8 disease: as they presented mild epilepsy, cognitive decline, mild learning disability, attention-deficit/hyperactivity disorder (ADHD), they developed a markedly protracted course of motor decline. Bioinformatic analyses of the compound heterozygous CLN8 gene variants were carried out. Most of the variants seem likely to act by compromising the structural integrity of regions within the protein. This in turn is expected to reduce the overall stability of the protein and render the protein less active to various degrees. The cases in our study confirmed and expanded the effect of compound heterozygous variants in CLN8 disease.
Single Nucleotide Polymorphisms in Amlodipine-Associated Genes and Their Correlation with Blood Pressure Control among South African Adults with Hypertension
Authors: Masilela Charity , Adeniyi Oladele Vincent , Benjeddou Mongi
Published: August 2022

Objective: This study describes the single nucleotide polymorphisms (SNPs) in amlodipine-associated genes and assesses their correlation with blood pressure control among South African adults with hypertension. Methods: In total, 304 hypertensive patients on amlodipine treatment belonging to the indigenous Swati, Xhosa and Zulu population groups of South Africa were recruited between June 2017 and June 2019. Participants were categorized into: controlled (blood pressure < 140/90 mmHg) and uncontrolled (blood pressure ≥ 140/90 mmHg) hypertension. Thirteen SNPs in amlodipine pharmacogenes with a high PharmGKB evidence base were selected and genotyped using MassArray (Agena BioscienceTM). Logistic regression was fitted to identify significant associations between the SNPs and blood pressure control with amlodipine. Results: The majority of the participants were females (76.6%), older than 45 years (89.1%) and had uncontrolled hypertension (52.3%). Of the 13 SNPs genotyped, five SNPs, rs1042713 (minor allele frequency = 45.9%), rs10494366 (minor allele frequency = 35.3%), rs2239050 (minor allele frequency = 28.7%), rs2246709 (minor allele frequency = 51.6%) and rs4291 (minor allele frequency = 34.4%), were detected among the Xhosa participants, while none were detected among the Swati and Zulu tribal groups. Variants rs1042713 and rs10494366 demonstrated an expression frequency of 97.5% and 79.5%, respectively. Variant TA genotype of rs4291 was significantly associated with uncontrolled hypertension. No association was established between blood pressure response to amlodipine and the remaining four SNPs. Conclusions: This study reports the discovery of five SNPs in amlodipine genes (rs2239050, rs2246709, rs4291, rs1042713 and rs10494366) among the indigenous Xhosa-speaking tribe of South Africa. In addition, the TA genotype of rs4291 was associated with blood pressure control in this cohort. These findings might open doors for more pharmacogenomic studies, which could inform innovations to personalised anti-hypertensive treatment in the ethnically diverse population of South Africa.
Examining the Diagnostic Yield of Tumour Testing and Qualifying Germline Concordance for Hereditary Cancer Variants in Patients with High-Grade Serous Carcinoma
Authors: Goebel Emily A. , Kerkhof Jennifer , Dzyubak Oleksandra , McLachlin C. Meg , McGee Jacob , Sadikovic Bekim
Published: August 2022

Despite advances in treatment, prognosis for most patients with high-grade serous carcinoma (HGSC) remains poor. Genomic alterations in the homologous recombination (HR) pathway are used for cancer risk assessment and render tumours sensitive to platinum-based chemotherapy and poly (ADP-ribose) polymerase inhibitors (PARPi), which can be associated with more favourable outcomes. In addition to patients with tumours containing BRCA1 or BRCA2 pathologic variants, there is emerging evidence that patients with tumours harbouring pathologic variants in other HR genes may also benefit from PARPi therapy. The objective of this study is to assess the feasibility of primary-tumour testing by examining the concordance of variant detection between germline and tumour-variant status using a custom hereditary cancer gene panel (HCP). From April 2019 to November 2020, HCP variant testing was performed on 146 HGSC formalin-fixed, paraffin-embedded tissue samples using next-generation sequencing. Of those, 78 patients also underwent HCP germline testing using blood samples. A pathogenic variant was detected in 41.1% (60/146) of tumours tested, with 68.3% (41/60) having either a BRCA1 or BRCA2 variant (n = 36), or BRCA1/2 plus a second variant (n = 5), and 31.2% (19/60) carrying a pathogenic variant in another HCP gene. The overall variant rate among the paired germline and tumour samples was 43.6% (34/78), with the remaining 56% (44/78) having no pathogenic variant detected in the germline or tumour. The overall BRCA1/2 variant rate for paired samples was 33.3% (26/78), with germline variants detected in 11.5% (9/78). A non-BRCA1/2 germline variant in another HCP gene was detected in 9.0% (7/78). All germline variants were detected in the tumour, demonstrating 100% concordance. These data provide evidence supporting the feasibility of primary-tumour testing for detecting germline and somatic variants in HCP genes in patients with HGSC, which can be used to guide clinical decision-making, and may provide opportunity for improving patient triage and clinical genetic referral practices.
Qualitative and Quantitative Detection of Mealworm DNA in Raw and Commercial Food Products Using Real-Time PCR
Authors: Natonek-Wiśniewska Małgorzata , Krzyścin Piotr , Koseniuk Anna
Published: August 2022

Considering food safety and an increasing public awareness of the ingredients, production process and origin of foods, the application of insects as food requires the development of tests for the reliable identification of their presence. The aim of the study was (1) the determination of appropriate modifications of the selected method for isolating the DNA of two life stages of mealworm, i.e., larva and adult, from commercial food products; (2) the determination of the method parameters for the qualitative and quantitative analysis of mealworm contents based on the detection of a species-specific mitochondrial DNA fragment, using real-time PCR; (3) the application of a method to test the commercial food products of mealworm. A total of nine species of adult insect were investigated (field cricket, Dubia cockroach, Madagascar cockroach, banded cricket, migratory locust, yellow mealworm, superworm, house fly and lacewing), theirlarvaes (yellow mealworms and superworms) and thirteen commercial food products (dried whole insects, powder and granules) representing various insect species and origins which were purchased from the European market. The obtained results showed that the efficiency of the modification of the DNA extraction method is dependent on the life stage of the mealworm. We proved the high sensitivity of the test, with the range of the method being 0.1–100%; we also proved the biological specificity in this range, and the linearity. The linearity of the test was also statistically verified using the Fisher–Snedecor test. One-way variance analysis showed statistically significant differences between the cT values of the two mealworm life stages studied, and similarly, between the threshold cycle (cT) values of adult forms. In contrast, for the inside group of mealworm larvae, there was no significant difference observed between the results of the cT values. The test is effective for processed food products and may be used to monitor food. The research proved the suitability of the applied method for the analysis of samples that are commercially available as food for exotic animals. The hereby-developed method is based on widely used laboratory techniques, and does not require any additional investment in equipment. The availabilityof such a methodallows for the verification of the accuracy of the declared species component of the food products.
Genetic Variation in DEAD-Box Helicase 20 as a Putative Marker of Recurrence in Propensity-Matched Colon Cancer Patients
Authors: Hobani Yahya H. , Almars Amany I. , Alelwani Walla , Toraih Eman A. , Nemr Nader A. , Shaalan Aly A. M. , Fawzy Manal S. , Attallah Samy M.
Published: August 2022

Variants of the DEAD-Box Helicase 20 (DDX20), one of the microRNAs (miRNAs) machinery genes, can modulate miRNA/target gene expressions and, hence, influence cancer susceptibility and prognosis. Here, we aimed to unravel the association of DDX20 rs197412 T/C variant with colon cancer risk and/or prognosis in paired samples of 122 colon cancer and non-cancer tissue specimens by TaqMan allelic discrimination analysis. Structural/functional bioinformatic analyses were carried out, followed by a meta-analysis. We found that the T allele was more frequent in cancer tissues compared to control tissues (60.2% vs. 35.7%, p < 0.001). Furthermore, the T variant was highly frequent in primary tumors with evidence of recurrence (73% vs. 47.5%, p < 0.001). Genetic association models, adjusted by age and sex, revealed that the T allele was associated with a higher risk of developing colon cancer under heterozygote (T/C vs. C/C: OR = 2.35, 95%CI = 1.25–4.44, p < 0.001), homozygote (T/T vs. C/C: OR = 7.6, 95%CI = 3.5–16.8, p < 0.001), dominant (T/C-T/T vs. C/C: OR = 3.4, 95%CI = 1.87–8.5, p < 0.001), and recessive (T/T vs. C/C-T/C: OR = 4.42, 95%CI = 2.29–8.54, p = 0.001) models. Kaplan–Meier survival curves showed the shift in the C > T allele to be associated with poor disease-free survival. After adjusting covariates using a multivariate cox regression model, patients harboring C > T somatic mutation were 3.5 times more likely to develop a recurrence (p < 0.001). A meta-analysis of nine studies (including ours) showed a higher risk of CRC (81%) in subjects harboring the T/T genotype than in T/C + C/C genotypes, supporting the potential clinical utility of the specified study variant as a biomarker for risk stratification in CRC cases. However, results were not significant in non-colorectal cancers. In conclusion, the DDX20 rs197412 variant is associated with increased colon cancer risk and a higher likelihood of recurrence in the study population.
Transcriptomic Immune Profiles Can Represent the Tumor Immune Microenvironment Related to the Tumor Budding Histology in Uterine Cervical Cancer
Authors: Le Tan Minh , Nguyen Hong Duc Thi , Lee Eunmi , Lee Donghyeon , Choi Ye Seul , Cho Junghwan , Park Nora Jee-Young , Han Hyung Soo , Chong Gun Oh
Published: August 2022

Tumor budding (TB) histology has become a critical biomarker for several solid cancers. Despite the accumulating evidence for the association of TB histology with poor prognosis, the biological characteristics of TB are little known about in the context related to the tumor immune microenvironment (TIME) in uterine cervical cancer (CC). Therefore, this study aimed to identify the transcriptomic immune profiles related to TB status and further provide robust medical evidence for clinical application. In our study, total RNA was extracted and sequenced from 21 CC tissue specimens. As such, 1494 differentially expressed genes (DEGs) between the high- and low-TB groups were identified by DESeq2. After intersecting the list of DEGs and public immune genes, we selected 106 immune-related DEGs. Then, hub genes were obtained using Least Absolute Shrinkage and Selection Operator regression. Finally, the correlation between the hub genes and immune cell types was analyzed and four candidate genes were identified (one upregulated (FCGR3B) and three downregulated (ROBO2, OPRL1, and NR4A2) genes). These gene expression levels were highly accurate in predicting TB status (area under the curve >80%). Interestingly, FCGR3B is a hub gene of several innate immune pathways; its expression significantly differed in the overall survival analysis (p = 0.0016). In conclusion, FCGR3B, ROBO2, OPRL1, and NR4A2 expression can strongly interfere with TB growth and replace TB to stratify CC patients.
The Association of Gender and Mortality in Geriatric Trauma Patients
Authors: Pandya Shreya , Le Timothy , Demissie Seleshi , Zaky Ahmed , Arjmand Shadi , Patel Nikhil , Moko Lilamarie , Garces Juan , Rivera Paula , Singer Kiara , Fedoriv Ivan , Garcia Zachery , Kennedy James , Makkapati Bhavana , Mukherjee Indraneil , Szerszen Anita , Gross Jonathan , Glinik Galina , Younan Duraid
Published: August 2022

The association of gender with mortality in trauma remains a subject of debate. Geriatric trauma patients have a higher risk of mortality compared to younger patients. We sought to evaluate the association of gender with mortality in a group of geriatric trauma patients presenting to an academic level 1 trauma center (trauma center designated by New York State capable of handling the most severe injuries and most complex cases). Methods: We performed a retrospective review of geriatric trauma patients who were admitted to our trauma center between January 2018 and December 2020. Data collected included vital signs, demographics, injury, and clinical characteristics, laboratory data and outcome measures. The study controlled for co-morbidities, injury severity score (ISS), and systolic blood pressure (SBP) in the ED. Multivariable logistic regression analysis was performed to evaluate the association of gender and mortality. Results: 4432 geriatric patients were admitted during the study period, there were 1635 (36.9%) men and 3859 (87.2%) were White with an average age of 81 ± 8.5 years. The mean ISS was 6.7 ± 5.4 and average length of stay was 6 ± 6.3 days. There were 165 deaths. Male gender (OR 1.94, 95% CI 1.38 to 2.73), ISS (OR 1.12, 95% CI 1.09 to 1.14), Emergency Department SBP less than 90 mmHg (OR 6.17, 95% CI 3.17 to 12.01), and having more than one co-morbidity (OR 2.28, 95% CI 1.55 to 3.35) were independently predictive of death on multivariable logistic regression analysis. Conclusion: Male gender, Emergency Department systolic blood pressure less than 90 mmHg, having more than one co-morbidity, and injury severity are independent predictors of mortality among geriatric trauma patients.
Artificial Intelligence Analysis of Ulcerative Colitis Using an Autoimmune Discovery Transcriptomic Panel
Authors: Carreras Joaquim
Published: August 2022

Ulcerative colitis is a bowel disease of unknown cause. This research is a proof-of-concept exercise focused on determining whether it is possible to identify the genes associated with ulcerative colitis using artificial intelligence. Several machine learning and artificial neural networks analyze using an autoimmune discovery transcriptomic panel of 755 genes to predict and model ulcerative colitis versus healthy donors. The dataset GSE38713 of 43 cases from the Hospital Clinic of Barcelona was selected, and 16 models were used, including C5, logistic regression, Bayesian network, discriminant analysis, KNN algorithm, LSVM, random trees, SVM, Tree-AS, XGBoost linear, XGBoost tree, CHAID, Quest, C&R tree, random forest, and neural network. Conventional analysis, including volcano plot and gene set enrichment analysis (GSEA), were also performed. As a result, ulcerative colitis was successfully predicted with several machine learning techniques and artificial neural networks (multilayer perceptron), with an overall accuracy of 95–100%, and relevant pathogenic genes were highlighted. One of them, programmed cell death 1 ligand 1 (PD-L1, CD274, PDCD1LG1, B7-H1) was validated in a series from the Tokai University Hospital by immunohistochemistry. In conclusion, artificial intelligence analysis of transcriptomic data of ulcerative colitis is a feasible analytical strategy.
Aging and Psychological Well-Being: The Possible Role of Inhibition Skills
Authors: Fusi Giulia , Palmiero Massimiliano , Lavolpe Sara , Colautti Laura , Crepaldi Maura , Antonietti Alessandro , Di Domenico Alberto , Colombo Barbara , Di Crosta Adolfo , La Malva Pasquale , Rozzini Luca , Rusconi Maria Luisa
Published: August 2022

(1) Background: Psychological well-being (PWB) is a multidimensional construct which is a key protective factor against chronic diseases in older adults. Numerous psychological and cognitive factors can influence older people’s PWB. However, while most studies have explored the effect of general cognition, only a few investigated the role of specific cognitive functions such as the efficiency of executive functions. Thus, the present study aimed to directly explore the relationships between PWB, age, and the core executive functions (namely working memory, flexibility, and inhibition). (2) Methods: A sample of 51 healthy older adults (age range: 60–88) were enrolled and tested both for PWB (by Ryff’s psychological well-being scale) and the core executive functions (by the Digit span backward, the Trail Making Test, and the Stroop test). (3) Results: Age and inhibitory skills are negatively correlated to the personal growth subscale of the PWB scale. Moreover, a hierarchical regression analysis showed that inhibition, but not working memory and flexibility, predicted the sense of continued growth and development (personal growth) of older adults, even after controlling for age and educational levels. Limitations and implications for future research and prevention interventions are discussed.
Neurological Disorders of Patients Living with HIV Hospitalized in Infectious Departments of the Specialist Hospital in Lower Silesia in Poland
Authors: Janocha-Litwin Justyna , Simon Krzysztof
Published: August 2022

Background and Objectives: Central nervous system (CNS) disorders are estimated to occur in approximately 10–20% of people living with HIV (PLWH). They are more commonly observed in newly diagnosed patients and in previously untreated patients or those refusing to undergo antiretroviral treatment. CNS diseases can also be the first manifestation of HIV/AIDS infection. The most common HIV-related central nervous system diseases (CNS-D) are CNS toxoplasmosis, CNS cryptococcosis, progressive multifocal leukoencephalopathy (PML), and HIV-associated encephalopathy treated as a neurocognitive disorder. Materials and Methods: A retrospective analysis of available medical records was performed on 476 patients hospitalised over a period from 2016 to 2021 and diagnosed with HIV/AIDS infection at the department of infectious diseases at the Provincial Specialist Hospital in Wroclaw. An additional criterion for selecting patients for the analysis was the performance of head imaging using computed tomography or magnetic resonance imaging on prospective patients. Results: Neurotoxoplasmosis, neurocryptococcosis, progressive multifocal leukoencephalopathy (PML), and neurosyphilis were the most common CNS diseases among the analysed group of patients. Based on radiological descriptions, other abnormalities, such as vascular changes or cortical and subcortical atrophy of multifactorial origin, not exclusively related to HIV infection, were also frequently observed. The most common neurological symptoms reported in the study group were headaches, limb paresis, and gait and balance disturbance. Conclusions: The clinical picture and epidemiology of neurological manifestations in the group of HIV-infected patients under assessment were similar to the results of other authors. Given the current epidemiological situation, diagnosis for HIV infection should be considered in patients admitted to neurological departments.
The Translation and Cross-Cultural Adaptation of the Pregnancy Physical Activity Questionnaire: Validity and Reliability of a Serbian Version (PPAQ-SRB)
Authors: Rovcanin Marija , Jankovic Svetlana , Mikovic Zeljko , Sipetic Grujicic Sandra , Ersk Ivana Rudic Biljic , Lackovic Milan , Dimitrijevic Dejan , Simanic Sara , Vujcic Isidora
Published: August 2022

Exercise during pregnancy has a positive effect on the health of both pregnant women and their fetuses. This study aimed to translate the Pregnancy Physical Activity Questionnaire (PPAQ) into the Serbian language and assess its validity and reliability among Serbian pregnant women. The study was conducted between October 2020 and March 2021 at the Obstetrics and Gynecology Clinic (Narodni Front), in Belgrade, Serbia. The PPAQ was translated according to a standardized methodology, and its internal consistency and construct and concurrent validity were assessed. The mean PPAQ score for the total amount of physical activity was 37.72 MET-h/week−1. Exploratory factor analysis of the Serbian PPAQ identified six factors similar to the original questionnaire that explained 70.26% of the data variance. The Cronbach’s alpha coefficient of the Serbian version of the PPAQ was 0.69. The two-week intraclass correlation coefficient (ICC) scores ranged from 0.768 to 0.930. We tested the evidence to assess the concurrent validity of the Serbian version of PPAQ (PPAQ-SRB) correlations with the International Physical Activity Questionnaire-long form (IPAQ-LF), and all domains of the PPAQ were significantly correlated with domains of the IPAQ-LF. The findings of our reliability and validity evaluation are consistent with those of prior studies, indicating that the PPAQ was successfully translated and implemented in the Serbian population and that its reliability was acceptable.
Factors Affecting Perceived Effectiveness of Government Response towards COVID-19 Vaccination in Occidental Mindoro, Philippines
Authors: Jou Yung-Tsan , Mariñas Klint Allen , Saflor Charmine Sheena , Young Michael Nayat , Prasetyo Yogi Tri , Persada Satria Fadil
Published: August 2022

The COVID-19 pandemic has caused several developing countries to fall behind on vaccination at the onset of the pandemic, thus affecting the mobility of easing restrictions and lowering virus transmission. The current study integrated the Protection Motivation Theory (PMT) and extended the Theory of Planned Behavior (TPB) to evaluate factors affecting the perceived effectiveness of government response towards COVID-19 vaccination in Occidental Mindoro. A total of 400 respondents from the municipalities of Occidental Mindoro answered the online questionnaires, which contained 61 questions. This study outlined the relationship between the dependent and independent variables using structural equation modeling (SEM). The results indicated that knowledge of COVID-19 vaccination had significant direct effects on its perceived severity. Subjective standards had significant adverse effects on willingness to follow. In addition, perceived behavioral control was discovered to impact willingness to follow positively. It also showed that perceived government response was significantly affected by adaptive behavior and actual behavior regarding the perceived government response. Meanwhile, it was found that the perceived government response had significant effects on perceived effectiveness. The current study is one of the first to study the factors that affect the perceived effectiveness of government response toward COVID- 19 vaccination.
Effects of Tai Chi and Walking Exercise on Emotional Face Recognition in Elderly People: An ERP Study
Authors: Zhang Xiaofei , Bao Jie , Yang Haoping , Zhang Zonghao , Shu Deming , Luo Li
Published: August 2022

Both tai chi and walking can improve the physical health of the elderly, but the effect on the emotional cognitive function of the elderly is unclear. To investigate the effect of long-term walking and tai chi exercise on the emotional cognitive function of the elderly, 63 subjects were recruited in this study according to age and exercise habits, including 16 in the youth control group, 15 in the elderly non-exercise control group, 17 in the elderly walking group, and 18 in the elderly tai chi group. The “learning–test paradigm” of emotional faces was used to measure the subjects’ ability to recognize and remember emotional (negative and neutral) faces. Behavioral and EEG data were recorded during the learning and testing phases. The results showed that there is aging in emotional cognition in older adults compared with younger adults. Long-term walking and tai chi exercise can delay the deterioration of emotional cognitive function in older adults to some extent. Both walking and tai chi exercise can delay the decline in aging-related emotional face recognition function to some extent. Walking exercise can delay the decline in aging-related emotional face memory function to some extent.
From Laboratory to Field: The Effect of Controlling Oscillations in Temperature on the Growth of Crops
Authors: Wang Jhih-Wei , Chen Chiachung
Published: August 2022

Crops are grown in natural conditions. Environmental factors such as temperature, humidity, and light irradiance have a regular pattern. A grower chamber is usually used to test the effect of environmental factors on the crops. If crops were cultivated under fixed environmental conditions, the results would be different from that for plants that are grown in a natural environment. Limits in the capability of an apparatus mean that most studies use a fixed temperature, a fixed relative humidity, or a fixed light intensity. In an accumulated temperature study, Pak choi and strawberry were used to compare the difference between fixed and oscillating temperature conditions in a growth chamber. There were three temperature patterns: the high-temperature (HVT) oscillation pattern (day: 22–32 °C; night: 19–25 °C), low-temperature (LVT) oscillation pattern (day: 21–29 °C; night: 19–23 °C), and the fixed temperature (CT) regime (day: 25 °C; night: 22 °C). The light intensity was 80 μmol∙m−2s−1. The environments used for the Pak choi experiments were the HVT oscillation pattern, the CT pattern, and the inside air temperature for a net house. The strawberries had three treatments: the HVT and LVT oscillation patterns, and the CT pattern. The fresh weight of total plants, the leaf area, the leaf number, and the chlorophyll content were measured for the two crops. For strawberries, the fresh weight of the leaves and the Brix value were measured. The experimental results show that there is a significant difference in the growth characteristics for these three regimes. The results of the growth characteristics of Pak choi and strawberry for an oscillating temperature are better than those for a fixed temperature environment with a statistically significant difference. For the biological and agricultural experiments, an oscillating temperature in the growth chamber realistically simulates a natural environment.
STIs during the COVID-19 Pandemic in Hungary: Gonorrhea as a Potential Indicator of Sexual Behavior
Authors: Lőrincz Kende , Meznerics Fanni Adél , Jobbágy Antal , Kiss Norbert , Madarász Mária , Belvon Laura , Tóth Béla , Tamási Béla , Wikonkál Norbert Miklós , Marschalkó Márta , Bánvölgyi András
Published: August 2022

The social distancing measures introduced due to the COVID-19 pandemic may have affected the sexual behavior of the population. We collected data retrospectively from the National STD Center of Hungary. The overall patient influx data of the STD Center and the number of patients diagnosed with syphilis, chlamydia, and gonorrhea infections were assessed in the three-month period of 2020 when the strict governmental lockdown was introduced in Hungary. Data were compared to the pre- and post-lockdown quarters of 2020 and matched to the respective quarters of 2018 and 2019. The number of patients diagnosed with syphilis and chlamydia infections in 2020 during the lockdown decreased compared to 2018 and 2019, while the number of gonorrhea cases increased. The lower number of STI screenings resulted in a significant decrease in asymptomatic syphilis and chlamydia case numbers. However, the growing number of gonorrhea cases in 2020 during lockdown highlights that sexual behavior remained unchanged regardless of restrictions. Therefore, gonorrhea may be considered as an indicator of STI incidences during the pandemic.
Targeted Primary and Secondary Preventive Strategies for Depression among Malaysian Pharmacy Students
Authors: A. Wahab Izyan A. , Goh Khang Wen , Zainal Zainol Akbar , Mohamed Yusof Najlaa Siham Mohamed , Huri Hasniza Zaman , Jacob Sabrina Anne , Mohamad Alwi Muhammad Najib Mohamad , Hashim Rosnani , Ahmad Hisham Shairyzah Ahmad , Jamil Nurdiana
Published: August 2022

The global depression burden has remained a challenge throughout the pre- and post-pandemic era. The pandemic effect has led to the spiraling of mental disorders among young people who will be the next generation of leaders. This study aims to identify university students’ sociodemographic, psychosocial and academic backgrounds and performance associated with depression symptoms for the development of primary and secondary preventive strategies for mental health. A cross-sectional study was conducted using an online questionnaire distributed to 19 institutions in Malaysia offering a Bachelor of Pharmacy degree program. The self-rated Depression Anxiety Stress Scale (DASS-42) was used to assess depression symptoms. Pearson’s chi-square test and Fisher’s exact test were used to assess the investigated variables with depression symptoms. Independent T-test and one-way ANOVA were used to compare means of depression score across variables. Binary logistic regression was employed to examine the relationship between the investigated variables and depression symptoms. A total of 610 pharmacy students participated, of which 47% (n = 289/610) were having depression symptoms. Students who smoke nicotine and those who have separated parents, family history of mental illness, and poor academic performance were associated with depression symptoms (p < 0.05). Differences in geographical areas, race and religion also showed significant associations with depression symptoms. Parental marital status, poor academic performance, history of mental illness and comorbidities were statistically predicting depression symptoms (p < 0.05). Primary preventive strategies allowing students to harness healthy coping skills for stress, nicotine-free campaigns and a holistic curriculum are warranted. Secondary measures on mindfulness and compassion skills activities to benefit students who experienced early life crises are highly recommended. Enforcing these targeted strategies in collaboration with health and social sectors should be the primary agenda of universities to ensure their uptake.
Does Environmental Interpretation Impact Public Ecological Flow Experience and Responsible Behavior? A Case Study of Potatso National Park, China
Authors: Tang Tiantian , Zhao Minyan , Wang Dan , Chen Xiangyu , Chen Wuqiang , Xie Chunwen , Ding Yan
Published: August 2022

Being responsible for ensuring nature preservation, environmental interpretation raises people’s awareness of nature preservation as a form of public service, and enhance their environmentally responsible behavior. Based on the flow theory, this study proposes a conceptual model of environmental interpretation impacts on visit motivation, ecological experience, environmental attitudes, and environmental behaviors. Selecting the users (visitors) of environmental interpretation at Potatso National Park in Shangri-La, Yunnan Province, China, we obtained 568 valid questionnaires and used Amos software to analyze a structural equation model to verify the model. The results indicate that the environmental interpretation plays a significant role in enriching the public’s ecological experience, which is an intermediary variable in which visiting motivation influences environmental attitudes and guides environmentally responsible behavior. The research suggests that national parks should strengthen the environmental interpretation facilities experiential and available, and adjust the configuration of the existing interpretation media in the three-dimensional structure of theme, space, and time, considering the motivation of the public visits, enriching ecological experience, and inspiring service details.
Assessing the Terrain Gradient Effect of Landscape Ecological Risk in the Dianchi Lake Basin of China Using Geo-Information Tupu Method
Authors: Wang Qiming , Yang Kun , Li Lixiao , Zhu Yanhui
Published: August 2022

The assessment of landscape ecological risk (LER) in different terrain gradients is beneficial to ecological environmental protection and risk management in different terrain gradients. Due to the impact of urban expansion, the landscape pattern of the Dianchi Lake basin (DLB) changed obviously, resulting in significant spatial difference of LER. At present, the LER assessment of the DLB is not clear, and the evolution mechanism of LER in different terrain gradients has not been revealed. Based on the LER assessment model, the geo-information Tupu method, the terrain niche gradient, and distribution index, this paper analyzed the LER and its terrain gradient effect in the DLB of China. The conclusions are as follows: (1) Since 1995, the land use type has mainly changed from grassland and cultivated land to construction land in the DLB of China. (2) The LERs in the DLB of China were mainly low, med low, and med high due to the transformation of land use type. The dominance distribution of the low and high LER was obviously constrained by terrain gradient. While the dominance distribution of med-low LER expanded to med-high terrain gradient, the dominance distribution of the med-high LER decreased to med-low terrain gradient. (3) The Tupu LERs were mainly a stable type of “medium” risk and anaphase change type of “med-high to medium” risk. The dominant distribution regions of the stable type, the prophase change type, and the continuous change type were relatively stable; the anaphase and middle change type expanded to the higher terrain gradient, and the repeated change type decreased to the med-high terrain gradient. In the process of ecological risk management and protection in the DLB, attention should be paid to the water area structure and LER control in med-high and high terrain gradients.
Water, Sanitation, and Hygiene (WASH) Practices and Outreach Services in Settlements for Rohingya Population in Cox’s Bazar, Bangladesh, 2018–2021
Authors: Faruque ASG , Alam Baharul , Nahar Baitun , Parvin Irin , Barman Ashok Kumar , Khan Soroar Hossain , Hossain M Nasif , Widiati Yulia , Hasan ASM Mainul , Kim Minjoon , Worth Martin , Vandenent Maya , Ahmed Tahmeed
Published: August 2022

(1) Background: This study aimed to investigate the existing water, sanitation, and hygiene (WASH) policy and practice of the study population and strengthen the evidence base by documenting changes in the WASH policy and practice over 3 years of the Rohingya refugee humanitarian crisis, Cox’s Bazar, Bangladesh. (2) Methods: A cross-sectional surveillance design was followed; the sampling of the study population included the Rohingya refugee population and neighborhood host nationals who required hospitalization soon after seeking care and enrolled into the diarrheal disease surveillance in diarrhea-treatment centers. Throughout the study period of 3 years, a total of 4550 hospitalized individuals constituted the study participants. (3) Results: Among the hospitalized Rohingya refugee population; the use of public tap water increased significantly from 38.5% in year 1 to 91% in year 3. The use of deep tube well water significantly changed from 31.3% to 8.2%, and the use of shallow tube well water reduced significantly from 25.8% to 0.4%. Households using water seal latrine were 13.3% in year 1 and increased significantly to 31.7% in year 3. ORS consumption at home changed significantly from 61.5% in the first year to 82.1% in third year. Multivariable analysis demonstrated patients’ age groups at 5 to 14 years, and 15 years and more, drinking non-tube well water, soap use after using toilet, use of non-sanitary toilet facility, father’s and mother’s lack of schooling, and some and severe dehydration were significantly associated with the Rohingya refugee population enrolled into the diarrheal disease surveillance. (4) Conclusion: The findings indicate significant advances in WASH service delivery as well as outreach activities by aid agencies for the Rohingya refugee population living in settlements.
Childbirth Satisfaction during the COVID-19 Pandemic in a Hospital in Southwestern Spain
Authors: González-Morcillo María , Tiburcio-Palos Esther , Cordovilla-Guardia Sergio , Santano-Mogena Esperanza , Franco-Antonio Cristina
Published: August 2022

Satisfaction, in relation to care received, is a good indicator of quality of care. The objective of this study was to analyze the degree of satisfaction with childbirth and postpartum care as reported by women from one hospital in southwestern Spain during the COVID-19 pandemic. Factors that influenced care were also examined. A cross-sectional study was carried out between the months of October 2020 and February 2021. Satisfaction was measured through the COMFORTS scale, validated in Spanish. A final sample of 116 women was included in the study. The mean age was 32.08 (±4.68) years. A total of 111 (95.69%) women were satisfied or very satisfied with the care received. The median satisfaction score was higher among multiparous women (187 (199–173)) than among primiparous women (174 (193–155.50)) (p = 0.003). Differences in satisfaction were found as a function of the use of epidural analgesia, being higher among women who had planned its use but ultimately did not use it (188 (172.50–199.75)) or who planned its use and did (186 (169.50–198)) than among those who had not planned to use epidural analgesia but ultimately received it (173.50 (187.50–146.25)) or those who did not use it, as planned, before childbirth (172 (157–185)) (p = 0.020). Overall satisfaction rate between SARS-CoV-2-negative women assisted was high. Parity and use of epidural analgesia were two factors influencing satisfaction scores in our sample.
Multidisciplinary Neuromuscular and Endurance Interventions on Youth Basketball Players: A Systematic Review with Meta-Analysis and Meta-Regression
Authors: Sacot Arnau , López-Ros Víctor , Prats-Puig Anna , Escosa Jesús , Barretina Jordi , Calleja-González Julio
Published: August 2022

The main aims of this systematic review with meta-analysis and meta-regression were to describe the effect of multidisciplinary neuromuscular and endurance interventions, including plyometric training, mixed strength and conditioning, HIIT basketball programs and repeated sprint training on youth basketball players considering age, competitive level, gender and the type of the intervention performed to explore a predictive model through a meta-regression analysis. A structured search was conducted following PRISMA guidelines and PICOS model in Medline (PubMed), Web of Science (WOS) and Cochrane databases. Groups of experiments were created according to neuromuscular power (vertical; NPV and horizontal; NPH) and endurance (E). Meta-analysis and sub-groups analysis were performed using a random effect model and pooled standardized mean differences (SMD). A random effects meta-regression was performed regressing SMD for the different sub-groups against percentage change for NPV and NPH. There was a significant positive overall effect of the multidisciplinary interventions on NPV, NPH and E. Sub-groups analysis indicate differences in the effects of the interventions on NPV and NPH considering age, gender, competitive level and the type of the intervention used. Considering the current data available, the meta-regression analysis suggests a good predictability of U-16 and plyometric training on jump performance. Besides, male and elite level youth basketball players had a good predictability on multidirectional speed and agility performance.
The Association between Bullying Victimization and Subjective Well-Being among Children: Does the Role of Child Religiosity Matter?
Authors: Massarwi Adeem Ahmad , Gross-Manos Daphna
Published: August 2022

Bullying is a major risk factor for poor psychological development for both children and adolescents worldwide. The current study, based on data from the International Survey of Children’s Well-Being (ISCWeB), explores the association between bullying victimization and subjective well-being as well as the moderating role of the child religiosity in this context among a sample of 2733 children aged 10–12 years old in Israel. Data was collected from children using self-reporting questionnaires, adopting a random stratified sampling method. A PROCESS moderation analysis was performed using SPSS for assessing the part played by child religiosity in moderating bullying victimization and the subjective well-being of children. This produced two key findings: first, children’s subjective well-being is negatively associated with bullying victimization; second, children’s religiosity serves as a protective factor by moderating the association between the child’s bullying victimization and subjective well-being. In light of the results, we recommend professionals who work with children to incorporate spiritual and religious resources into school-based interventions aiming at strengthening the child’s inner resilience and help overcome difficulties in their lives, based on a religious coping strategies.
The Role of Novel Bladder Cancer Diagnostic and Surveillance Biomarkers—What Should a Urologist Really Know?
Authors: Malinaric Rafaela , Mantica Guglielmo , Lo Monaco Lorenzo , Mariano Federico , Leonardi Rosario , Simonato Alchiede , Van der Merwe André , Terrone Carlo
Published: August 2022

The aim of this review is to analyze and describe the current landscape of bladder cancer diagnostic and surveillance biomarkers. We researched the literature from 2016 to November 2021 to find the most promising new molecules and divided them into seven different subgroups based on their function and location in the cell. Although cystoscopy and cytology are still the gold standard for diagnosis and surveillance when it comes to bladder cancer (BCa), their cost is quite a burden for national health systems worldwide. Currently, the research is focused on finding a biomarker that has high negative predictive value (NPV) and can exclude with a certainty the presence of the tumor, considering missing it could be disastrous for the patient. Every subgroup has its own advantages and disadvantages; for example, protein biomarkers cost less than genomic ones, but on the other hand, they seem to be less precise. We tried to simplify this complicated topic as much as possible in order to make it comprehensible to doctors and urologists that are not as familiar with it, as well as encourage them to actively participate in ongoing research.
Parenting Stress, Maternal Self-Efficacy and Confidence in Caretaking in a Sample of Mothers with Newborns (0–1 Month)
Authors: Tognasso Giacomo , Gorla Laura , Ambrosini Carolina , Figurella Federica , De Carli Pietro , Parolin Laura , Sarracino Diego , Santona Alessandra
Published: August 2022

A mother’s responses to her newborn and her confidence in the child’s caretaking depend on her attachment security, general parental stress, and perceived self-efficacy. However, few studies have analyzed maternal confidence in caretaking and how it is influenced by some mothers’ characteristics. We aimed to examine the association between maternal adult attachment and confidence in a child’s caretaking and to understand whether this relationship was mediated by parenting stress and maternal self-efficacy. The sample consisted of 96 mothers with a mean age of 33 years with newborn children aged between 3 and 30 days. The instruments used were the Experiences in Close Relationships-Revised (ECR-R), the Mother and Baby Scale (MABS), the Parenting Stress Index Short Form (PSI-SF), and the Maternal Self-Efficacy Questionnaire (MEQ). The results showed a positive association between attachment avoidance and lack of confidence in caretaking, and this association was mediated by parenting stress. Conversely, attachment anxiety appeared not to influence confidence in caretaking, and maternal self-efficacy did not appear to mediate the relationship between attachment and confidence in the caretaking of infants. Our results could guide new research in studying confidence in caretaking and enable healthcare professionals to recognize at-risk situations early from the first month after childbirth.
Channeled PM10, PM2.5 and PM1 Emission Factors Associated with the Ceramic Process and Abatement Technologies
Authors: Celades Irina , Sanfelix Vicenta , López-Lilao Ana , Gomar Salvador , Escrig Alberto , Monfort Eliseo , Querol Xavier
Published: August 2022

A sampling methodology and a mathematical data treatment were developed that enable to determine not only total suspended particulates (TSP) emitted at channeled sources but also the PM10, PM2.5, and PM1 mass fractions (w10, w2.5, and w1) and emission factors (E.F.), using a seven-stage cascade impactor. Moreover, a chemical analysis was performed to identify the elements present in these emissions. The proposed methodology was applied to different stages of the ceramic process, including ambient temperature (milling, shaping, glazing) and medium–high-temperature (spray-drying, drying, firing, and frit melting) stages. In total, more than 100 measurements were performed (pilot scale and industrial scale), which leads to a measurement time of 1500 h. Related to the mass fractions, in general, the mean values of w10 after the fabric filters operated at high performance are high and with little dispersion (75–85%), and it is also observed that they are practically independent of the stage considered, i.e., they are not significantly dependent on the initial PSD of the stream to be treated. In the case of the fine fraction w2.5, the behavior is more complex (w2.5: 30–60%), probably because the only variable is not the cleaning system, but also the nature of the processed material. Regarding abatement measures, the use of high-efficiency cleaning systems considerably reduces the emission factors obtained for fractions PM10, PM2.5, and PM1. In reference to chemical analysis, the presence of ZrO2 and Ni in the spray-drying and pressing stages, the significant concentration of ZrO2 in the glazing stage, the presence of Pb, As, and Zn in the firing stage, and the presence of Zn, Pb, Cd, and As compounds in the frits manufacturing should all be highlighted. Nevertheless, it should be pointed out that the use of some compounds, such as cadmium and lead, has been very limited in the last years and, therefore, presumably, the presence of these elements in the emissions should have been also reduced in the same way.