bluuebunny/crossref_metadata_2025 huggingface dataset:
Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development
Authors: Zheng Mengxue , Andersen Claus Yding , Rasmussen Frida Roikjer , Cadenas Jesús , Christensen Søren Tvorup , Mamsen Linn Salto
Published: October 2023

Introduction

Granulosa cells (GCs) and theca cells (TCs) play a pivotal role in human ovarian steroidogenesis, facilitating the conversion of cholesterol into sex steroids that regulate normal reproductive function. This study aims to explore the expression patterns of key enzymes that govern human ovarian steroidogenesis throughout follicle development, employing both genomic and immunological methodologies.

Methods

Follicles and GCs obtained from women undergoing ovarian tissue cryopreservation (OTC) and in vitro fertilisation treatment were utilized. Gene expression data were obtained from a Chinese study using RNA sequencing and from microarray data generated in our laboratory to comprehensively analyse gene expression profiles across distinct stages of follicular development. To corroborate the localisation of key enzymes within GCs and TCs, immunohistochemistry analyses utilizing colourimetric and fluorescent techniques were conducted.

Results

Steroidogenesis-related enzymes displayed low gene expression levels during early follicle development. However, a notable upregulation of HSD3B2 was observed in GCs as follicles progressed to the antral/preovulatory stage, confirmed consistently using both microarray and RNA sequencing methodologies. Furthermore, immunohistochemical analyses effectively demonstrated that HSD3B2 were not only expressed in GCs, but co-localised with CYP17A1 within a specific subset of TCs surrounding human small antral follicles. Contributing to an enhanced progesterone production during the second half of the follicular phase was a significant upregulation of CYB5A in both microarray and RNA-seq datasets as follicles transition from the antral stage to the pre-ovulatory stage. Moreover, an augmented expression of DHCR24 and LDLR in both types of data, along with HMGCR expression expression in the microarray data, indicates increased substrate availability for ovarian steroidogenesis.

Discussion

This study confirms and extends that GCs gradually augment expression of HSD3B2 thereby enhancing their capacity for progesterone synthesis as follicles reach the size of selection at around 10 mm in diameter. This is supported by the expression CYB5A and possibly augmented availability of steroid precursors. A subset of TCs exhibit concurrent expression of CYP17A1 and HSD3B2, collectively contributing to the synthesis of 17-hydroxyprogesterone. These data significantly enhance our understanding of the dynamic regulation of progesterone throughout the process of follicular development.
Single-cell transcriptomics reveals subtype-specific molecular profiles in Nrf2-deficient macrophages from murine atherosclerotic aortas
Authors: Sarad Katarzyna , Stefańska Monika , Kraszewska Izabela , Szade Krzysztof , Sluimer Judith C. , Błyszczuk Przemysław , Dulak Józef , Jaźwa-Kusior Agnieszka
Published: October 2023

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcriptional regulator of antioxidant and anti-inflammatory response in all cell types. It also activates the transcription of genes important for macrophage function. Nrf2 activity declines with age and has been closely linked to atherosclerosis, but its specific role in this vascular pathology is not clear. Atherosclerotic plaques contain several macrophage subsets with distinct, yet not completely understood, functions in the lesion development. The aim of this study was to analyze the transcriptome of diverse Nrf2-deficient macrophage subpopulations from murine atherosclerotic aortas. Mice with transcriptionally inactive Nrf2 in Cdh5-expressing cells (Nrf2Cdh5tKO) were used in the experiments. These mice lack transcriptional Nrf2 activity in endothelial cells, but also in a proportion of leukocytes. We confirmed that the bone marrow-derived and tissue-resident macrophages isolated from Nrf2Cdh5tKO mice exhibit a significant decline in Nrf2 activity. Atherosclerosis was induced in Nrf2Cdh5tKO and appropriate control mice via adeno-associated viral vector (AAV)-mediated overexpression of murine proprotein convertase subtilisin/kexin type 9 (Pcsk9) in the liver and high-fat diet feeding. After 21 weeks, live aortic cells were sorted on FACS and single-cell RNA sequencing (scRNA-seq) was performed. Unsupervised clustering singled out 13 distinct aortic cell types. Among macrophages, 9 subclusters were identified. Differential gene expression analysis revealed cell subtype-specific expression patterns. A subset of inflammatory macrophages from atherosclerotic Nrf2Cdh5tKO mice demonstrated downregulation of DNA replication genes (e.g. Mcm7, Lig1, Pola1) concomitant with upregulation of DNA damage sensor Atr gene. Atherosclerotic Nrf2Cdh5tKO Lyve1+ resident macrophages showed strong upregulation of IFN-stimulated genes, as well as changes in the expression of death pathways-associated genes (Slc40a1, Bcl2a1). Furthermore, we observed subtype-specific expression of core ferroptosis genes (e.g. Cp, Hells, Slc40a1) in inflammatory versus tissue resident macrophages. This observation suggested a link between ferroptosis and inflammatory microenvironment appearing at a very early stage of atherogenesis. Our findings indicate that Nrf2 deficiency in aortic macrophages leads to subtype-specific transcriptomic changes associated with inflammation, iron homeostasis, cell injury or death pathways. This may help understanding the role of aging-associated decline of Nrf2 activity and the function of specific macrophage subtypes in atherosclerotic lesion development.
Fcγ receptor binding is required for maximal immunostimulation by CD70-Fc
Authors: Dadas Osman , Allen Joel D. , Buchan Sarah L. , Kim Jinny , Chan H. T. Claude , Mockridge C. Ian , Duriez Patrick J. , Rogel Anne , Crispin Max , Al-Shamkhani Aymen
Published: October 2023

Introduction

T cell expressed CD27 provides costimulation upon binding to inducible membrane expressed trimeric CD70 and is required for protective CD8 T cell responses. CD27 agonists could therefore be used to bolster cellular vaccines and anti-tumour immune responses. To date, clinical development of CD27 agonists has focussed on anti-CD27 antibodies with little attention given to alternative approaches.

Methods

Here, we describe the generation and activity of soluble variants of CD70 that form either trimeric (t) or dimer-of-trimer proteins and conduct side-by-side comparisons with an agonist anti-CD27 antibody. To generate a dimer-of-trimer protein (dt), we fused three extracellular domains of CD70 to the Fc domain of mouse IgG1 in a ‘string of beads’ configuration (dtCD70-Fc).

Results

Whereas tCD70 failed to costimulate CD8 T cells, both dtCD70-Fc and an agonist anti-CD27 antibody were capable of enhancing T cell proliferation in vitro. Initial studies demonstrated that dtCD70-Fc was less efficacious than anti-CD27 in boosting a CD8 T cell vaccine response in vivo, concomitant with rapid clearance of dtCD70-Fc from the circulation. The accelerated plasma clearance of dtCD70-Fc was not due to the lack of neonatal Fc receptor binding but was dependent on the large population of oligomannose type glycosylation. Enzymatic treatment to reduce the oligomannose-type glycans in dtCD70-Fc improved its half-life and significantly enhanced its T cell stimulatory activity in vivo surpassing that of anti-CD27 antibody. We also show that whereas the ability of the anti-CD27 to boost a vaccine response was abolished in Fc gamma receptor (FcγR)-deficient mice, dtCD70-Fc remained active. By comparing the activity of dtCD70-Fc with a variant (dtCD70-Fc(D265A)) that lacks binding to FcγRs, we unexpectedly found that FcγR binding to dtCD70-Fc was required for maximal boosting of a CD8 T cell response in vivo. Interestingly, both dtCD70-Fc and dtCD70-Fc(D265A) were effective in prolonging the survival of mice harbouring BCL1 B cell lymphoma, demonstrating that a substantial part of the stimulatory activity of dtCD70-Fc in this setting is retained in the absence of FcγR interaction.

Discussion

These data reveal that TNFRSF ligands can be generated with a tunable activity profile and suggest that this class of immune agonists could have broad applications in immunotherapy.
Drifter observations of surface currents in the Cyprus Gyre
Authors: Poulain P.-M. , Menna M. , Mauri E. , Pirro A. , Hayes D. R. , Gildor H.
Published: October 2023

Wavelet ridge analysis was applied to the data of drifters caught in the Cyprus Gyre in the eastern Mediterranean Sea to study surface currents at low (rotating circulation) and high (near-inertial motion) frequencies. Drifter observations indicate that the orbital speed is highest at about 30 km from the gyre center (reaching ~45 cm/s) and that the Rossby number in the gyre can be as high as 0.25. Drifters also show that strong near-inertial currents with speed of 35-40 cm/s can exceed the gyre rotation speed and that the background vorticity inside the gyre generally shifts the effective inertial frequency to values smaller than the local inertial frequency. As a result, the near-inertial currents are subinertial and are trapped in the mesoscale vortex. For example, the near-inertial motions in the Cyprus Gyre in June 2017 reached 40 cm/s in its inner core with a red shift of ~0.1 cpd, while on its edge they were limited to 10-20 cm/s, under similar wind forcing. This difference is due to trapping of the near-inertial energy inside the gyre. The observed inertial currents are largely driven by the local wind, especially when the effective inertial frequency becomes diurnal and resonance with wind diurnal variability occurs. A damped slab model of the inertial currents in the mixed layer is only partially successful in simulating the observations.
Comparison of glial fibrillary acidic protein-immunoglobulin G-associated myelitis with myelin oligodendrocyte glycoprotein-immunoglobulin G-associated myelitis
Authors: Sun Mengyang , Liu Hao , Zhu Bingqing , Liu Yang , Li Aijia , Wang Limei
Published: October 2023

Objective

Glial fibrillary acidic protein-immunoglobulin G (GFAP-IgG)-associated myelitis and myelin oligodendrocyte glycoprotein-IgG (MOG-IgG)-associated myelitis have rarely been compared. Therefore, this study aimed to explore the clinical, laboratory, and imaging features of them to identify the differences.

Methods

Overall, 14 and 24 patients with GFAP-IgG-and MOG-IgG-associated myelitis, respectively, were retrospectively screened and included in the study.

Results

Among the 14 patients with GFAP-IgG-associated myelitis, the condition was more common in males (71.4%), with a median age of onset of 36.5 years, and more common in adults than in children (35.7%). In contrast, among the 24 patients with MOG-IgG-associated myelitis, the condition was equally divided between males and females, with a median age of onset of 9.5 years and more in children (66.7%) than in adults. The median age of onset of GFAP-IgG-associated myelitis was later than that of the MOG-IgG group. Isolated myelitis was rare in both groups. Elevated cerebrospinal fluid (CSF) protein levels were more prevalent in patients with GFAP-IgG-associated myelitis (64.3%) than in those with MOG-IgG-associated myelitis (16.7%) (p < 0.05), whereas patchy gadolinium enhancement of the cerebral lesion site was less common in patients with GFAP-IgG-associated myelitis than in those with MOG-IgG associated myelitis (p < 0.05). Six patients had a combination of other neurological autoantibodies, the specific mechanism of the overlapping antibodies remains unclear.

Conclusion

Cerebrospinal fluid analysis and gadolinium enhanced MRI examination may help to distinguish the two kinds of myelitis.
Thyroid nodule malignancy is associated with increased non-invasive hepatic fibrosis scores in metabolic subjects
Authors: Crudele Lucilla , Novielli Fabio , De Matteis Carlo , Petruzzelli Stefano , Suppressa Patrizia , Berardi Elsa , Antonica Gianfranco , Piazzolla Giuseppina , Sabbà Carlo , Graziano Giusi , Moschetta Antonio
Published: October 2023

Introduction

Thyroid cancer incidence is increasing, and adiposity-related conditions are gaining space in its pathogenesis. In this study, we aimed to detect any anthropometric, biohumoral, and clinical features that might be associated with thyroid nodule malignancy, potentially representing novel non-invasive markers of thyroid cancer.

Materials and methods

The study was conducted in a group of 142 consecutive outpatients (47 men and 95 women) who underwent fine-needle aspiration biopsy/cytology (FNAB/C) due to suspicion of malignancy from January 2018 to September 2022. We compared lipid and glycemic blood profiles as well as non-invasive liver fibrosis indexes such as aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio (AAR), AST to platelet ratio index (APRI), and fibrosis index based on four factors (FIB-4) between patients with benign and malignant newly diagnosed nodules. Then, we performed receiver operating characteristic (ROC) analysis to assess their best cutoff values for discrimination of malignant nodules and chi-squared test to evaluate the association of specific dysmetabolic conditions with malignancy. To understand whether and to what degree dysmetabolic conditions increased the risk of thyroid nodule malignancy, we also calculated the odds ratio (OR) of the main biomarkers.

Results

After FNAB/C, 121 (85%) patients were diagnosed with benign thyroid nodules, while 21 (15%) individuals were diagnosed with thyroid cancer. Comparing patients with benign and malignant nodules, we found that individuals with thyroid cancer exhibited increased body mass index (BMI) (p = 0.048) and fasting plasma glucose (p = 0.046). Intriguingly, considering non-invasive scores for liver fibrosis, subjects with thyroid cancer presented increased AAR (p < 0.001) and APRI (p = 0.007), and these scores were associated with malignancy (p < 0.005) with OR = 7.1 and OR = 5, respectively. Moreover, we showed that only in the cancer group, low levels of vitamin D correlated with stigmata of impaired metabolism.

Discussion

In our study, AAR and APRI scores were associated with thyroid nodule malignancy and could be used to predict it and to speed up the diagnostic process. From a pathogenic point of view, we speculated that metabolic-associated fatty liver disease (MAFLD) along with hyperglycemia and vitamin D deficiency may represent putative drivers of thyroid carcinogenesis.
PD-1 blockade combined with gemcitabine plus nab-paclitaxel is superior to chemotherapy alone in the management of unresectable stage III/IV pancreatic cancer: a retrospective real-world study
Authors: Cheng Daoan , Hu Jing , Wu Xiaoyu , Wang Banglu , Chen Rui , Zhao Weiqing , Fang Cheng , Ji Mei
Published: October 2023

Background

Pancreatic cancer (PC) is widely recognized as one of the most malignant forms of cancer worldwide. Monotherapy with immune checkpoint inhibitors (ICI) has shown limited efficacy in treating this disease. There was controversy surrounding whether combining ICI with chemotherapy provided superior outcomes compared to chemotherapy alone.

Methods

In this study, patients diagnosed with unresectable stage III/IV pancreatic cancer (PC) were classified as receiving programmed cell death protein 1 (PD-1) blockade plus gemcitabine and nab-paclitaxel (AG regimen) (PD-1/chemo, n=27, 50.9%) or chemotherapy alone (chemo, n=26, 49.1%) arm. The primary study endpoints included progression-free survival (PFS) and overall survival (OS), with an additional assessment of treatment-related adverse events graded as three or higher. Chi-square (χ2) statistics were employed to analyze the clinical differences between the two groups, while Kaplan-Meier curves were used to assess the difference in PFS and OS. Statistical significance was defined as P-values less than 0.05 (P < 0.05).

Results

The median follow-up duration was 22 months (range 1-28 months). In the PD-1/chemo arm, the median PFS was eight months, whereas it was 3.5 months in the chemo arm (HR=0.459, 95% CI: 0.252-0.846, P=0.002). Furthermore, the median OS was 15 months in the PD-1/chemo arm and eight months in the chemo arm (HR=0.345, 95% CI: 0.183-0.653, P<0.001). Within the PD-1/chemo arm, 15 (55.6%) patients experienced grade 3 treatment-related adverse events, compared to 13 (50.0%) patients in the chemo arm.

Conclusions

PD-1 blockade combined with nab-paclitaxel plus gemcitabine demonstrated superior efficacy to chemotherapy alone for unresectable stage III/IV PC patients. Future studies were warranted to identify immunosensitive patient subgroups within the PC population, ultimately leading to the development of more efficacious therapeutic strategies.
A systematic review of data sources for artificial intelligence applications in pediatric brain tumors in Europe: implications for bias and generalizability
Authors: Tozzi Alberto Eugenio , Croci Ileana , Voicu Paul , Dotta Francesco , Colafati Giovanna Stefania , Carai Andrea , Fabozzi Francesco , Lacanna Giuseppe , Premuselli Roberto , Mastronuzzi Angela
Published: October 2023

Introduction

Europe works to improve cancer management through the use of artificialintelligence (AI), and there is a need to accelerate the development of AI applications for childhood cancer. However, the current strategies used for algorithm development in childhood cancer may have bias and limited generalizability. This study reviewed existing publications on AI tools for pediatric brain tumors, Europe's most common type of childhood solid tumor, to examine the data sources for developing AI tools.

Methods

We performed a bibliometric analysis of the publications on AI tools for pediatric brain tumors, and we examined the type of data used, data sources, and geographic location of cohorts to evaluate the generalizability of the algorithms.

Results

We screened 10503 publications, and we selected 45. A total of 34/45 publications developing AI tools focused on glial tumors, while 35/45 used MRI as a source of information to predict the classification and prognosis. The median number of patients for algorithm development was 89 for single-center studies and 120 for multicenter studies. A total of 17/45 publications used pediatric datasets from the UK.

Discussion

Since the development of AI tools for pediatric brain tumors is still in its infancy, there is a need to support data exchange and collaboration between centers to increase the number of patients used for algorithm training and improve their generalizability. To this end, there is a need for increased data exchange and collaboration between centers and to explore the applicability of decentralized privacy-preserving technologies consistent with the General Data Protection Regulation (GDPR). This is particularly important in light of using the European Health Data Space and international collaborations.
Hydroponic lettuce defective leaves identification based on improved YOLOv5s
Authors: Jin Xin , Jiao Haowei , Zhang Chao , Li Mingyong , Zhao Bo , Liu Guowei , Ji Jiangtao
Published: October 2023

Achieving intelligent detection of defective leaves of hydroponic lettuce after harvesting is of great significance for ensuring the quality and value of hydroponic lettuce. In order to improve the detection accuracy and efficiency of hydroponic lettuce defective leaves, firstly, an image acquisition system is designed and used to complete image acquisition for defective leaves of hydroponic lettuce. Secondly, this study proposed EBG_YOLOv5 model which optimized the YOLOv5 model by integrating the attention mechanism ECA in the backbone and introducing bidirectional feature pyramid and GSConv modules in the neck. Finally, the performance of the improved model was verified by ablation experiments and comparison experiments. The experimental results proved that, the Precision, Recall rate and mAP0.5 of the EBG_YOLOv5 were 0.1%, 2.0% and 2.6% higher than those of YOLOv5s, respectively, while the model size, GFLOPs and Parameters are reduced by 15.3%, 18.9% and 16.3%. Meanwhile, the accuracy and model size of EBG_YOLOv5 were higher and smaller compared with other detection algorithms. This indicates that the EBG_YOLOv5 being applied to hydroponic lettuce defective leaves detection can achieve better performance. It can provide technical support for the subsequent research of lettuce intelligent nondestructive classification equipment.
Three-dimensional visualization of the vascular bundle in a branched bamboo node
Authors: Li Shan , Yang Qianying , Wang Yangao , Shang Lili , Yang Shumin , Liu Xing’e , Ma Qianli , Cao Zixiong
Published: October 2023

Bamboo is a natural vascular bundle (VB) reinforced composite material used in more than 10 fields such as construction and furniture. The nodes in bamboo are crucial to its mechanical properties, but understanding of its performance is limited by lack of knowledge about the three-dimensional (3D) structure of the node. This work aimed to non-destructively identify the multi-dimensional characteristics of the VB in a bamboo branched node (BN) using X-ray microtomography (µCT). The VB was segmented from the BN using deep learning combined with the Watershed algorithm. The 3D model reconstruction and characterization of the VB were also conducted. It was found that the structure of VBs showed significant changes along the height of the BN. The VBs formed a complex 3D structure, VBs of the culm are connected with those of the branch, and the connectivity of the conducting tissue and fibers was 88.91% and 99.95%, respectively. The conducting tissue and the fibers had similar shapes but varying thicknesses, which enabled VBs to perform both water transport and mechanical support functions. The volumes fraction of parenchyma, fibers, and conducting tissue in the BN were 61.3%, 35.3%, and 3.4%, respectively, but the tissue proportion of the different heights of the BN varied from each other. The nodal ridge was a mechanical weak point of the BN, with a maximum fibers proportion of 43.8%. This study contributes to understanding the relationship of VBs between the branch and the culm. It provides a structural perspective for understanding the mechanical properties of BN and a theoretical basis for optimizing bamboo utilization efficiency.
Assessment of Hazardous Gaming in children and its dissimilarities and overlaps with Internet Gaming Disorder
Authors: Kewitz Sonja , Leo Katharina , Rehbein Florian , Lindenberg Katajun
Published: October 2023

Background and aims

Children have been vastly overlooked in Internet Gaming Disorder (IGD) and Hazardous Gaming research so far. The diagnoses are listed in different ICD-11 chapters (addiction vs. problematic health condition) and are thus considered as distinct constructs. However, screening tools for children do not exist yet. We aimed to investigate the psychometric properties of an existing IGD screening tool modified to also assess Hazardous Gaming in children. Further, we aimed to compare the dissimilarity and overlap between (subclinical) IGD and Hazardous Gaming in children.

Methods

The study analyzed data from a mixed school and clinical sample. Data from N = 871 children aged between 8 and 12 years of age (M = 10.3, SD = 0.90) were analyzed. Data were collected via the Video Game Dependency Scale (CSAS) in its parent report version, which was adapted to assess Hazardous Gaming symptoms in addition to the IGD symptoms. Item analyses and reliability and factor analyses were conducted on the Hazardous Gaming version.

Results

The results show that the adapted CSAS version that assesses Hazardous Gaming symptoms in children mostly shows acceptable psychometric properties. Explorative Factor Analysis (EFA) shows a two-factor structure with one factor of higher order. Additionally, results show that 35.2% of all children meeting the threshold for Hazardous Gaming exclusively meet criteria for Hazardous Gaming but not for (subclinical) IGD. Vice versa, 91.3% of children with IGD also meet the criteria for Hazardous Gaming.

Discussion

Hazardous Gaming and (subclinical) IGD are distinct constructs with some overlaps and might have a temporal relation. We recommend adding four items to assess Hazardous Gaming using the CSAS and further evaluate the validity. The assessment of Hazardous Gaming in children is crucial because it might occur earlier than subclinical or full-syndrome IGD.
Health staff perceptions of patient safety and associated factors in hospitals in Vietnam
Authors: Thu Nguyen Thi Hoai , Anh Bui Thi My , Ha Nguyen Thi Thu , Tien Doan Ngoc Thuy , Giang Pham Huong , Nga Tran Thi , Nam Nguyen Hoang , Hung Phung Thanh
Published: October 2023

Introduction

Patient safety is a global challenge of preventing and mitigating medical errors which might harm patients during their course of treatment and care. This study was employed to contribute to the existing literature aimed to assess patient safety culture among health staff and to determine predictors of health staff perceptions of patient safety in hospitals in Vietnam.

Methods

A cross-sectional study was conducted in three hospitals of Vietnam with a total of 763 participants. This study used the Hospital Patient Safety Scale developed by the American Health and Quality Research Organization.

Results

In general, 8 of 12 patient safety dimensions in two hospital; and 10 of 12 dimensions in a third hospital had average scores of 60% and above positive responses. The communication openness and organizational learning dimensions were found to be significant different when comparing hospitals. Regarding sample characteristics, department (subclinical department) and health staff positions (nurses/technicians, pharmacists) were significant predictors in the total model including three hospitals (R2 = 0.07).

Conclusion

This study reported that communication openness and organization learning are two aspects that need to be improved they are strongly related to patient safety culture and to knowledge exchange among health staff. It has been suggested that hospitals should deliver patient safety training courses and establish a supportive learning environment to improve these challenges.
Uptake of and intention to use oral pre-exposure prophylaxis for HIV among pregnant and post-natal women in Eswatini: a cross-sectional survey
Authors: Khumalo Philisiwe Ntombenhle , Mkhonta Siphiwesihle Sibonisiwe , Kindandi Kikanda , Matse Sindy , Dlamini Phinda Brian , Tukei Vincent , Machekano Rhoderick , Woelk Godfrey
Published: October 2023

Introduction

In Eswatini, HIV incidence among women of childbearing age is 1.45%. Eswatini introduced oral pre-exposure prophylaxis (PrEP) for HIV prevention in 2016 and requires that all HIV-negative pregnant and post-natal women (PPW) visiting health care facilities be offered PrEP.

Methods

Between September-November 2021, we conducted a survey among HIV-negative PPW from 16 purposively selected healthcare facilities in the Hhohho and Shiselweni regions in Eswatini. We interviewed consenting HIV-negative PPW using a structured questionnaire to collect data on PrEP knowledge, attitudes, intentions, and practices, as well as information on partner HIV status and stigma. Multivariate logistic regression was used to determine predictors of PrEP use and intention, adjusted for significant covariates.

Results

Of 1,484 PPW women approached, 1,149 consented and were interviewed, of whom 704 (61.3%) were post-partum and 445 (38.7%) pregnant. The median age was 25 years [Interquartile Range (IQR) = 21–30 years], with 533 (46.4%) 18–24 years old. Among the 1,149 women, 930 (80.7%) had ever heard about PrEP; 635 (55.3%) had knowledge about PrEP; 183 (15.9%) were currently using PrEP; and 285 (24.8%) had ever used PrEP. Increased odds of PrEP use were associated having HIV-positive male partner (aOR:7.76, 95%CI 3.53- 17.04); positive attitudes to PrEP (aOR:1.56, 95%CI: 1.02–2.40); and high self-efficacy (aOR:1.49, 95%CI:1.13–1.98). Among 864 women who never used PrEP, 569 (65.3%) intended to use PrEP in the future. Odds of intention to use PrEP were higher among women with low levels of education (aOR:2.23, 95% CI: 1.32–3.77); who ever heard about PrEP (aOR:1.69, 95%CI: 1.12–2.56); and had high self-efficacy (aOR:1.57, 95%CI: 1.31–1.87). Regarding stigma, among all women, 759 (66%) either agreed or strongly agreed that people would think they have HIV if they were to use PrEP; 658 (57.3%) reported they would be labelled as having multiple sex partners; 468 (40.7%) reported that their partner would think they are having risky sex with other people. Of 102 women who had discontinued PrEP, a majority stopped due to side effects 32 (35.2%).

Conclusion

Only about 50% of women had knowledge of PrEP, and PrEP uptake among PPW was low, though intention to use appeared high. More efforts to reduce stigma and promote PrEP use, including adequate information on side effects, are needed.
Eugenol nanoemulsion reduces Listeria monocytogenes biofilm by modulating motility, quorum sensing, and biofilm architecture
Authors: Balasubramanian Brindhalakshmi , Xue Jingyi , Luo Yangchao , Upadhyay Abhinav
Published: October 2023

Listeria monocytogenes is a major foodborne pathogen in the United States that is capable of forming sanitizer-tolerant biofilms on diverse food contact surfaces and under varying temperature conditions. A plethora of research in the last decade has explored the potential of phytochemicals as antibiofilm agents. However, the low solubility of phytochemicals is a significant challenge that needs to be addressed to develop plant-based disinfectants that can be applied in the industry for controlling L. monocytogenes biofilms and improving food safety. This study investigated the efficacy of eugenol nanoemulsion (EGNE) in inhibiting biofilm formation in two strains of L. monocytogenes (Scott A and AT19115) on stainless steel surfaces at two temperatures (25 or 10°C). In addition, the effect of EGNE on pathogen motility, extracellular polymeric substances (EPS) production, eDNA production, and quorum sensing activity during biofilm formation was studied using standard bioassays. Moreover, the efficacy of EGNE in killing mature L. monocytogenes biofilm was also investigated against both the strains and temperature combinations. All experiments had a completely randomized design with duplicate samples and were repeated at least three times. EGNE had a particle size of ~75 nm, a polydispersity index of 0.25, and a high negative surface charge. EGNE 700 mg/L inhibited L. monocytogenes biofilm formation significantly by ~1.89 log in 72 h at 25°C and ~1.25 log on day 16 at 10°C, when compared to control (p < 0.05). EGNE at 2,750 mg/L concentration completely inactivated (~7 log CFU/coupon reduction as compared to control) L. monocytogenes biofilm cells developed at 25 or 10°C as early as 1 min of treatment time (p < 0.05). In addition, EGNE was able to significantly reduce the motility, EPS, eDNA production, and quorum sensing activity which plays a major role in biofilm formation. Both L. monocytogenes Scott A and AT19115 strains exhibited similar sensitivity to EGNE treatments. The results suggest that EGNE could potentially be used as a natural sanitizer to effectively control L. monocytogenes biofilms in food processing environments.
On the Intersection of Computational Geometry Algorithms with Mobile Robot Path Planning
Authors: Latif Ehsan , Parasuraman Ramviyas
Published: October 2023

In the mathematical discipline of computational geometry (CG), practical algorithms for resolving geometric input and output issues are designed, analyzed, and put into practice. It is sometimes used to refer to pattern recognition and to define the solid modeling methods for manipulating curves and surfaces. CG is a rich field encompassing theories to solve complex optimization problems, such as path planning for mobile robot systems and extension to distributed multi-robot systems. This brief review discusses the fundamentals of CG and its application in solving well-known automated path-planning problems in single- and multi-robot systems. We also discuss three winning algorithms from the CG-SHOP (Computational Geometry: Solving Hard Optimization Problems) 2021 competition to evidence the practicality of CG in multi-robotic systems. We also mention some open problems at the intersection of CG and robotics. This review provides insights into the potential use of CG in robotics and future research directions at their intersection.
Discovering Non-Linear Boolean Functions by Evolving Walsh Transforms with Genetic Programming
Authors: Rovito Luigi , De Lorenzo Andrea , Manzoni Luca
Published: October 2023

Stream ciphers usually rely on highly secure Boolean functions to ensure safe communication within unsafe channels. However, discovering secure Boolean functions is a non-trivial optimization problem that has been addressed by many optimization techniques: in particular by evolutionary algorithms. We investigate in this article the employment of Genetic Programming (GP) for evolving Boolean functions with large non-linearity by examining the search space consisting of Walsh transforms. Especially, we build generic Walsh spectra starting from the evolution of Walsh transform coefficients. Then, by leveraging spectral inversion, we build pseudo-Boolean functions from which we are able to determine the corresponding nearest Boolean functions, whose computation involves filling via a random criterion a certain amount of “uncertain” positions in the final truth table. We show that by using a balancedness-preserving strategy, it is possible to exploit those positions to obtain a function that is as balanced as possible. We perform experiments by comparing different types of symbolic representations for the Walsh transform, and we analyze the percentage of uncertain positions. We systematically review the outcomes of these comparisons to highlight the best type of setting for this problem. We evolve Boolean functions from 6 to 16 bits and compare the GP-based evolution with random search to show that evolving Walsh transforms leads to highly non-linear functions that are balanced as well.
Wheat-Seed Variety Recognition Based on the GC_DRNet Model
Authors: Xing Xue , Liu Chengzhong , Han Junying , Feng Quan , Lu Qinglin , Feng Yongqiang
Published: October 2023

Wheat is a significant cereal for humans, with diverse varieties. The growth of the wheat industry and the protection of breeding rights can be promoted through the accurate identification of wheat varieties. To recognize wheat seeds quickly and accurately, this paper proposes a convolutional neural network-based image-recognition method for wheat seeds, namely GC_DRNet. The model is based on the ResNet18 network and incorporates the dense network idea by changing its residual module to a dense residual module and introducing a global contextual module, reducing the network model’s parameters and improving the network’s recognition accuracy. Experiments were conducted on the self-constructed wheat-seed dataset and the publicly available dataset CIFAR-100 by combining GC_DRNet with network models such as ResNet18, ResNet34, ResNet50, and DenseNet121. The GC_DRNet model achieved a recognition accuracy of 96.98% on the wheat-seed dataset, which was improved by 2.34%, 1.43%, 2.05%, and 1.77% compared to ResNet18, ResNet34, ResNet50, and DenseNet121, respectively. On the CIFAR-100 dataset, the recognition accuracy of the GC_DRNet model was 80.77%, which improved the accuracy of ResNet18, ResNet34, ResNet50, and DenseNet121 by 8.19%, 1.6%, 9.59%, and 16.29%, respectively. Analyzing the confusion-matrix results of the wheat-seed dataset, the average recognition precision of the test set was 97.02%, the recall rate was 96.99%, and the F1 value was 96.98%. The parameter size of the GC_DRNet model was smaller than that of the other three models, only 11.65MB. The experimental results show that the GC_DRNet has a high level of recognition accuracy and detection capability for images of wheat seeds and provides technical support for wheat-seed identification.
The Effect of Cropping Systems on Environmental Impact Associated with Winter Wheat Production—An LCA “Cradle to Farm Gate” Approach
Authors: Mukosha Chisenga Emmanuel , Moudrý Jan , Lacko-Bartošová Magdaléna , Lacko-Bartošová Lucia , Eze Festus Onyebuchi , Neugschwandtner Reinhard W. , Amirahmadi Elnaz , Lehejček Jiří , Bernas Jaroslav
Published: October 2023

The demand for wheat production is increasing and is associated with environmental effects. To sustain the increased demand, there is a need to find sustainable methods of wheat production. The choice of cropping system can significantly affect the environmental burden of agricultural production systems. This study presents the results of monitoring emission loads resulting from winter wheat cultivation under different cropping systems: organic unfertilized (ORG), organic fertilized (ORG-F), conventional unfertilized (CON), and conventional fertilized (CON-F). The system boundaries include all the processes from “cradle to farm gate” and the functional unit was 1 kg of wheat grain. The primary data were obtained from experimental field trials and secondary data from Ecoinvent v3.5, WFLDB, and Agri-footprint v5.0 databases. The results of this study are related to eight impact categories. The SimaPro 9.2.0.1 software and ReCiPe Midpoint (H) V1.13/Europe Recipe H were used for calculation. The results show that fertilized variants recorded higher environmental impacts compared to the unfertilized variants. The results indicate that ORG-F was more environmentally friendly compared to the CON-F variant at the expense of lower yields. Overall, ORG imposes the lowest environmental impact and is deemed to be more environmentally friendly.
The Use of Biodegradable Film in the Cultivation of Soybean with a Short Growing Season as an Example of Agro-Innovation in a Sustainable Agriculture System
Authors: Sikora Adrian , Klimek-Kopyra Agnieszka , Kulig Bogdan
Published: October 2023

The aim of the study was to assess the yield of three soybean varieties of different earliness classes (Merlin, Coraline, and Viola) grown using two sowing dates (early vs. optimal) and different technologies (soil protected with biodegradable film vs. without soil protection–conventional cultivation). A three-year (2019–2021) field experiment was conducted at the Bayer Technical Advisory Center in Chechło, Poland (50°23′ N 18°44′ E). The three-factor experiment was set up in a randomized split-plot design in three replicates. The experimental factors were (i) sowing date, (ii) cultivar, and (ii) cultivation technology. The effect of agrotechnical factors and their interaction with the weather on selected biometric traits and seed yield was assessed. The results indicated that the weather conditions and its interaction with agrotechnical factors significantly influenced the biometric traits and seed yield of soybean. Optimal hydrothermal conditions significantly increased analyzed parameters and seed yield. However, too much rainfall in August had negative effects on biometric traits. It was proved that, early sowing adversely affected soybean yield. Sowing at the optimal date, i.e., the end of April, resulted in a yield of 3.8 t ha−1. The use of biodegradable film in the year with more rainfall increased soybean yield by 1 t ha−1 compared to the year with less rainfall. The early ‘Merlin’ cultivar grown in the system with biodegradable film produced significantly more pods and seeds per plant and a higher pod weight per plant. The cultivars with a longer growing season (‘Viola’ and ‘Coraline’) responded negatively to cultivation in the modern technology. The use of biodegradable film is recommended for cultivars with a short growing season, sown at the optimal time and in regions with moderate to high rainfall totals during the growing season.
Reproductive Biology of Solanum orbiculatum ssp. orbiculatum, an Australian Endemic Bush Tomato
Authors: Ahmad Nabil , Chamas Ali , Trethowan Richard
Published: October 2023

The Australian tomato Solanum orbiculatum ssp. orbiculatum is an edible bush tomato endemic to the more arid areas of Western Australia, South Australia, and the Northern Territory. Breeding system data indicate that the plants are potentially self-compatible but are unable to carry out spontaneous autogamy or agamospermy. The flower is protogynous, as the stigma become receptive to pollen germination while still in bud condition and the anthers do not release pollen immediately after anthesis. This arrangement is a simple and common way to avoid too much self-pollination, favours cross pollination, and would allow forced bud pollination for hybrid development. The floral structure and morphology of this species can also encourage cross pollination, as the stigma is mostly exserted above the anther’s tips. In an attempt to examine the hypothesis of a positive correlation between pollen grain size and style length, we found a statistically significant difference between the pollen size of the long-styled and short-styled flowers. Pollen in vitro germination and viability tests have been optimised to facilitate effective breeding work on this species. A modified Brewbake and Kwack (BK) medium supplemented with 20% sucrose and 2.5% PEG 4000 has been found to be the most efficient media components for the in vitro germination of viable pollen grains. Alternatively, Alexander’s and acetocarmine (1%) stains have shown the highest positive correlation with the in vitro pollen germination test and, therefore, can be used as quick tests for checking pollen viability. Moreover, pollen grains stored for three months under 4 °C and dry conditions can be used efficiently to effect fertilisation in breeding programs, as it can maintain more than 50% of the original viability. This study will contribute to understanding the evolution and systematic relationships of species and for founding effective conservation programs. Furthermore, understanding the reproductive biology of this species is also of interest because of its potential for tomato breeding.
Microbial Community Shifts with Soil Properties and Enzyme Activities in Inter-/Mono-Cropping Systems in Response to Tillage
Authors: Lu Peina , Zhao Cai , Yin Wen , Hu Falong , Fan Zhilong , Yu Aizhong , Fan Hong
Published: October 2023

No-till and cereal–legume intercropping have been recognized as favorable cropping practices to increase crop yields while maintaining soil quality in arid and semiarid environments, but the biological mechanisms are poorly understood. The present study aimed to determine the response of yields, soil properties, enzyme activities, and microbial community diversity and composition in mono- and inter-cropping under conventional and no-tillage conditions. We initiated a field experiment in Wuwei, a typical arid area of China, in 2014. Soil was sampled in August 2022 and, yields, soil properties, enzyme activities, and the microbial community diversity and composition were determined in the maize and pea strips in inter- and mono-cropping systems. Results revealed that the maize and pea strips in the no-till intercropping significantly increased yields, total and organic carbon stocks, decreased NO3−-N, and obtained the highest total and organic P in the soil. No-tillage significantly enhanced the Shannon index and Pielou evenness of the bacterial community and total microbial community over conventional tillage, with the α-diversity of the bacterial community and total microbial community distinctly higher in the NTIM treatment than in the CTIM treatment. The α-diversity of the total microbial community was significantly related to yield, soil IC and OC, and the α-diversity of the archaea community was significantly related to soil TC, TC/TP, TN/TP, and BX. Meanwhile, the α-diversity of the eukaryote community was significantly related to soil yield, soil TC/TP. Both no-tillage and intercropped maize significantly increased the abundance of archaea phylum Thaumarchaeota and bacterial phylum Nitrospirae, and were significantly positively associated with soil OC and NH4+-N, benefiting nitrogen fixation of intercropped pea from the atmosphere under the no-tillage cereal/legume intercropping. No-till intercropping was conducive to the accumulation of organic carbon, while decreasing the abundance of Proteobacteria, Acidobacteria, and Verrucomicrobia. Limited soil enzyme activities (ACP, ALP, DP, NAG, BG, AG, CB) led to decreases in organic carbon turnover and utilization. Intercropping altered soil microbial community diversity and composition due to changes in soil properties and enzyme activities. These findings suggest that no-tilled cereal–legume intercropping is a sustainable cropping practice for improving soil properties and enhancing microbial (archaea, bacterial, eukaryota) diversity, but the persistence is not conducive to rapid turnover of soil nutrients due to limited enzyme activities.
Wild Avian Gut Microbiome at a Small Spatial Scale: A Study from a Mediterranean Island Population of Alectoris rufa
Authors: Guerrini Monica , Tanini Dalia , Vannini Claudia , Barbanera Filippo
Published: October 2023

This research is one of the few comparative descriptions at an intraspecific level of wild non-passerine microbiomes. We investigated for the first time the gut microbiome of red-legged partridges (Alectoris rufa) using fecal pellets in order to provide a more informed management. We focused on a small Italian population consisting of two demes (WEST, EAST) separated by about 20 km on the opposite sides of Elba Island. Given the small spatial scale, we set up a sampling protocol to minimize contamination from environmental bacteria, as well as differences due to variations in—among others—habitat, season, and age of feces, that could possibly affect the investigation of the three Elban sites. We found a significant divergence between the WEST and EAST Elban subpopulations in terms of microbial composition and alpha diversity. Although most represented bacterial phyla were the same in all the sites (Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes), microbiomes displayed a much higher diversity in western than in eastern partridges. This result might be related to locally diverging individual physiological needs and/or to different intensities in past releases of captive-bred birds between the two sides of Elba. We suggest that the two subpopulations should be treated as distinct management units.
Selected Aspects of Reproductive Behavior of American Mink (Neovison vison) under Artificial Conditions
Authors: Seremak Beata , Pilarczyk Bogumiła , Wojciechowska Aleksandra , Tomza-Marciniak Agnieszka
Published: October 2023

Observation and behavioral analysis of animals can be one of the factors taken into consideration when assessing the welfare of animals kept in artificially created breeding conditions. Disturbed welfare and prolonged exposure to stress can lead to the emergence of abnormal behaviors, including reproductive issues. The aim of this study was to examine the mating behavior of male American mink, with regard to the duration of mating and any potential differences with regard to time of day, mating period, number of mating attempts, and time spent with the female. The study material consisted of 12 one-year-old male American minks with pearl coloring and 60 two-year-old females. Observations were conducted using images captured using externally mounted cameras. The video material capturing the activity of males and females during the mating period was analyzed using the Behawior program. The obtained results indicate the presence of individual differences in copulation duration. It was observed that the copulation time increases with successive days of the breeding season and with number of copulations. It was also observed that a longer time spent with the female to initiate copulation, and behaviors such as rubbing or sniffing the female, were associated with a shorter copulation time. The above-mentioned studies provide specific guidelines regarding the management of matings on the farm, demonstrating the feasibility of conducting matings both in the hours before and after noon.