bluuebunny/crossref_metadata_2025 huggingface dataset:
PHILOSOPHER AND PEDAGOGUE OF CULTURE SERGEI GESSEN AND THE IDEA OF THE UNIVERSITY
Authors: Lorenzova Jitka
Published: February 2022

This analytical study is dedicated to notable Russian philosopher in exile and theoretical pedagogue of culture Sergei Iosifovich Gessen (1887-1950), who is a somewhat forgotten personality in Czech pedagogy today. It aims to elaborate on those of Hessen's ideas which are at the heart of his concept of pedagogy and of the university as an institution, and which can provide an important frame of reference for the criticism of today’s instrumental approach to education and university reform. Gessen is presented as an ever-inspiring, timeless thinker who developed his philosophical anthropology and general theory of education on the principles of neo-Kantian critical philosophy, and as a representative of humanist educational ideals. The analysis is supplemented by a contradictory perception of Gessen's legacy in the Czech pedagogical environment, which has been negatively impacted by the social and political conflicts of the 20th century. The study employs theoretical analysis of primary and secondary sources, as well as hermeneutic-interpretive methods.
TEACHERS´ SELF-EFFICACY IN INCLUSIVE EDUCATION
Authors: Brozmanova Monika , Cabanova Mariana , Lynch Zuzana
Published: February 2022

The perceived teacher’s self-efficacy is considered to be one of the important indicators of the professional quality as well as the educational process. In the study, the authors present the results of research focused on capturing the teacher’s self-efficacy in inclusive practice. The research sample consisted of 375 primary school teachers from all regions of Slovakia, more than 80% of whom stated the length of practice more than ten years. The authors monitored the perception of respondents’ self-efficacy in three factors: in inclusive teaching, in cooperation, and in managing pupils' behavior. In all three factors, the results indicate a high level of their perceived self-efficacy in inclusive practice. Based on the data analysis, the relationship between the positioning of teachers in career levels and their perceived self-efficacy has not been proven. In the article, we deal more with the search for connections between the perception of self-efficacy in individual factors and the completion of educational topics that cover the issue of inclusion of pupils.
DISCOVERY-TECHNIQUE-BASED TEACHING IN SECONDARY VOCATIONAL EDUCATION
Authors: Seben Zatkova Tímea , Bobak Zuzana
Published: February 2022

One of many alternatives available for teaching is inquiry-based teaching. In this type of teaching, the student is in the role of a researcher and the teacher in the role of the creator of learning activities and in certain phases also a guide for students. The aim of this study was to map the opinions and experiences of teachers with inquiry-based teaching and its implementation into secondary vocational education teaching. The research methodology was based on a questionnaire survey among secondary school teachers with the largest representation from the Nitra region. The results show that although awareness of inquiry-based teaching reaches 55% among the teachers, the practical implementation is low.
CITIZENSHIP EDUCATION OF ADULTS IN SELECTED COUNTRIES OF EUROPE – AN OVERVIEW STUDY
Authors: Beran Sladkayova Michaela , Neusarova Jana
Published: February 2022

The presented study focuses on the institutional provision and implementation of citizenship education in selected countries that have a developed concept of citizenship education and address this issue. At the same time, we consider these countries as examples of good practice and inspiration for the Slovak concept of citizenship education of adults, which, from our point of view, is not sufficiently developed. At the same time, it must be emphasized that citizenship education of adults is significantly influenced by the overall socio-cultural development and history of society in each country, which is also the reason why countries differ in their citizenship education of adults’ models.
THE STORY OF OLGA KACHULKOVA (KHMELEVA) «ROBINSON IN THE RUSSIAN FOREST» AT THE RECEPTION OF M. OSORGIN
Authors: Stepanova Nadezhda S.
Published: February 2022

The author of the article focuses on the story of the «forgotten» Russian writer of the late XIX – early XX centuries Olga Kachulkova (Khmeleva) «Robinson in the Russian Forest», written in the tradition of the bildungsroman, and its perception by M. Osorgin, about which he spoke in his autobiographical book «Vremena» («Times»). The story «Robinson in the Russian Forest» is devoted to the problems of the formation, personal development and maturation of the protagonist during the three years of his voluntary and forced robinsonade in the Russian forest. M. Osorgin’s childhood impressions of the book he read independently at the age of seven about the robinsonade of Russian boys, about the harmonious relationship between man and the surrounding nature, contributed to the formation of a free soul and the construction of a national image of the world. The article defines the place for the story of the adventures of two boys from different social strata who lived for three years in a huge wild forest, in the autobiographical book of M. Osorgin. The author of the article understands reception as a dialogical interaction of the perceiving consciousness and the literary text involved in its orbit, while maintaining the sovereignty of each of the «voices». М. Osorgin’s interpretation of the story «Robinson in the Russian Forest» preserved for future readers the very existence of this book, and also helped to explore the life-creating and intertextual aspects of the reception of the story in their dynamics, the poetics and philosophy of robinsonade in the understanding of M. Osorgin.
BRANDING AS A LINGUOCULTURAL PHENOMENON: A PRAGMATIC AND SEMANTIC ASPECT
Authors: Medvedeva Aliya A.
Published: February 2022

The article touches upon the analysis of the pragmаtic and semantic aspect that is entwined into the phenomenon of branding. The phenomenon of branding reflects a significant cultural layer of the modern consumer society. The essence of the brand is central to understanding the concept of the brand itself. It is a representation of semantic and pragmatic characteristics that merge into a single pragmatic-semantic unit. To reveal the presuppositions inherent in the essence of the brand, it is necessary to be able to “understand” the target audience and recognize an individual as a representative of this target audience with their interests and needs. Understanding the essence of the brand and decoding the presuppositions embedded in it is impossible without correct perception of the pragmatic-semantic aspects of the entire semiotic situation, within which the brand functions as a linguocultural phenomenon. That means that the concept of a brand in the article is considered not only as a marketing concept when promoting commercial products, but is understood more broadly as a linguocultural phenomenon, whose pragmatics is formed by merging key semantic layers – syntactics, semantics, semiotics – into it in the context of the modern linguocultural situation. The semantics of the brand and its decoding through the disclosure of the linguo-semiotic aspects integrated into the brand name during its formation largely depend on the pragmatic potential, which sends the participants of the communication directly to the context of speech use. Thus, we can say that the formation of the brand’s message is based on two aspects engaged in forming the semantic load - linguistic and paralinguistic, implemented through the pragmatics of the brand, whose formation, in turn, takes place at the above mentioned three semantic levels.
Strong Representation Learning for Weakly Supervised Object Detection
Authors: Li Min Song Yu, , Weidong Du , Yujie He , Yao Gou , Zhaoqing Wu , Yilong Lv
Published: February 2022

To solve the problem that the feature maps generated by feature extraction network of traditional weakly supervised learning object detection algorithm is not strong in feature, and the mapping relationship between feature space and classification results is not strong, which restricts the performance of object detection, a weakly supervised object detection algorithm based on strong representation learning is proposed in this paper. Due to enhance the representation ability of feature maps, the algorithm weighted the channels of feature maps according to the importance of each channel, to strengthen the weight of crucial feature maps and ignore the significance of secondary feature maps. Meanwhile, a Gaussian Mixture distribution model with better classification performance was used to design the object instance classifier to enhance further the representation of the mapping between feature space and classification results, and a large-margin Gaussian Mixture (L-GM) loss was designed to increase the distance between sample categories and improve the generalization of the classifier. For verifying the effectiveness and advancement of the proposed algorithm, the performance of the proposed algorithm is compared with six classical weakly supervised target detection algorithms on VOC datasets. Experiments show that the weakly supervised target detection algorithm based on strong representation learning has outperformed other classical algorithms in average accuracy (AP) and correct location (CorLoc), with increases of 1.1%~14.6% and 2.8%~19.4%, respectively.
Poradniki metodyczne jako źródło wiedzy nauczycieli w edukacji wczesnoszkolnej
Authors: Jakubowicz-Bryx Anna
Published: February 2022

The last few years were a time when a number of publications and methodological guides for early school education teachers were launched on the Polish market. This paper presents a debate on the technical and methodological quality of such publications. The considerations are based on empirical surveys carried out among early school education teachers, assuming that a methodological guide is the right tool in a teacher’s hands, whose task is to create conditions suitable for individual education of students and initiating various types of children’s activity as far as integrated education is concerned.
Edukacja wczesnodziecięca w Japonii – przemiany programowe i organizacyjne
Authors: Cylkowska-Nowak Mirosława
Published: February 2022

The aim of this paper is the analysis and the description of early childhood education and care in Japan. The one country comparative study explores curricula and organization aspects of different education and care solutions. “Education” and “care” are combined in the phrase to underline that services for young children have to combine care, development and learning opportunities. The paper consists five parts, which describe: genesis of early childhood education and care in Japan; early adaptation and elaboration of curricula; development of new education and care solutions after second world war; organizational aspects of early childhood education and care in Japan; and development prognosis.
O proletariado em Friedrich Engels
Authors: Monzelli Arthur , Cagnin José Guilherme , Navarro Érica Zavanella
Published: February 2022

Este artigo pretende analisar se a obra de Friedrich Engels lança luz sobre o problema da escassez de moradias e das condições de vida e trabalho do proletariado hoje. Para responder a tal questão, levantam-se duas hipóteses: a primeira é que os problemas do proletariado, estudados por Engels no século XIX, ainda não estão esgotados, uma vez que se fundamentam no modo de produção capitalista; a segunda hipótese diz respeito ao fato de que, para superar a questão da moradia e as atuais condições precárias do proletariado, é necessário a suprassunção [Aufhebung] do capitalismo. Por fim, o artigo fundamenta-se, teórica e metodologicamente, no materialismo histórico dialético, ou seja, examina a realidade concreta, descrevendo-a teoricamente e articulando-a às condições de vida, moradia e trabalho do proletariado hoje para, assim, compreendê-las em suas múltiplas determinações. O artigo resulta de duas pesquisas em andamento. Uma delas investiga a situação do proletariado da educação pública paulista. A outra, analisa o fenômeno da população em situação de rua.
“Teoria” da reprodução social em debate
Authors: Pereira Alana Andreia
Published: February 2022

Neste artigo foram recuperadas algumas análises das principais autoras da “teoria” da reprodução social, não com o objetivo de realizar uma análise imanente de seus trabalhos, e sim, de analisar as principais contribuições, desde uma postura feminista marxiana e socialista, baseada no uso do método de Marx, o materialismo histórico dialético que, guarda certa ortodoxia e não dogmatismos, entendendo que se trata não de uma “teoria” da reprodução social ou “feminismo” da reprodução social, mas de uma categoria analítica valiosa para a luta anticapitalista pela emancipação humana, a categoria reprodução social.
Valve-in-valve transcatheter aortic valve replacement: the challenge of the next future
Authors: Annibali Gianmarco , Scrocca Innocenzo , Musumeci Giuseppe
Published: February 2022

In recent years, an increasing number of bioprostheses have been implanted, and in the near future more and more patients will be candidates for reoperation due to structural deterioration of the valve. Valve-in-valve transcatheter aortic valve replacement (ViV TAVR) has become a safe and effective alternative to surgery and is currently approved for higher-risk, inoperable patients. From the most recent studies, early mortality has decreased and improvements in symptoms and quality of life of treated patients have been documented. ViV TAVR is a complex procedure that can present many pitfalls and therefore must be performed in high volume centers and with experienced staff because the risk of peri- and post-procedural complications is much higher than TAVR on native valve. In this review, we analyze the main procedural issues reported in the literature during ViV TAVR procedures: elevated postprocedural gradients, coronary obstruction and thrombosis of the leaflets of the bioprosthesis. Because of the opening of TAVR to younger and younger patients, thus with a longer life expectancy than the durability of the bioprosthesis, the next challenge will be the management of the lifetime strategy of patients with aortic stenosis, as the first type of intervention will influence all future therapeutic choices of our patient.
Using the Research Results in Implementing Educational Programs for the Direction «Library and Information Activities»
Authors: Merkulova A. Sh. , Savkina S. V.
Published: February 2022

Research activity is an integral part of the educational process at the university. The formation of educational programs of higher education includes not only the development of the main components of the of students training, but also the consideration of scientific directions and research in the field of culture, including library and information activities. The purpose of the article is to prove that there is a clearrelationship between the research activities of university teachers, the research work ofstudents and the formation of educational programs with the approbation of the methodology for analyzing this relationship.

Within the framework of the conducted research, the results of the research activities of students and teachers – final qualification papers and publications of teachers of the Faculty of Information and Library Technologies of the Kemerovo State Institute of Culture (KEMGIK) were analyzed; the information obtained was compared with educational programs for bachelor’s and master’s degrees in library and information activities.

The analysis of the topics of the final qualifying works is carried out, the leading directions of student research are identified. The correspondence of the disciplines of the curriculum to the research directions is presented. Based on the analysis of publications and an online survey of faculty teachers, scientific interests have been identified, which are reflected in the educational process. The influence of the results of students and teachers researchs on the formation and implementation of educational programs of library and information activities is considered. The analysis of research activities at the faculty has shown that in the course of the educational process, the results of students and teachers researches are used to modernize the content of disciplines, develop new courses, as well as retraining and advanced training programs for library staff. The authors prove that there is a clear relationship between the research activities of university teachers, the research work of students and the formation of educational programs.
Распространение вариантов вируса SARS-COV-2, вызывающих озабоченность (VOC) и интерес (VOI) на основе количества их геномов, депонированных в базу данных GISAID за неделю с 29.01.2022 г. по 04.02.2022 г.
Authors: Дмитриева Людмила , Краснов Ярослав , Чумачкова Елена , Осина Наталья , Сафронов Валентин , Иванова Александра , Карнаухов Игорь , Караваева Татьяна , Щербакова Светлана , Кутырев Владимир
Published: February 2022

В обзоре представлен анализ геновариантов вируса SARS-CoV-2, вызывающих озабоченность (VOC) и интерес (VOI) на основе их геномов в базе GISAID за неделю с 29.01.2022 г. по 04.02.2022 г.
Accessibility of Essential Amino Acids, Synthesis of SARS-CoV-2 Virus Polyproteins, and the Outcomes of COVID-19 Pathogenesis
Authors: Ponomarenko Sophia
Published: February 2022

Aim. To identify: a risk factor influencing the development of COVID-19 disease. Compare the amino acid composition of animal and plant proteins with non-structural polyproteins of the SARS-CoV-2 virus. To analyze: the impact of dietary essential amino acids (EAAs) on the development of infectious disease COVID-19. Materials and methods. The scientific data and information needed for this analysis was found in publications and media available on the Internet, as well as taken from statistical databases, using the necessary keywords for a single tag or in various combinations of them. Statistical samples were formed from sources and facts available on the Internet. Amino acid sequences of proteins were obtained from databases (https://www.ncbi.nlm.nih.gov/, https://www.uniprot.org/uniprot/). Results and discussion. Analysis of statistical data and assessment of nutritional factors during the development of the 22-month pandemic in different countries confirmed that the outcome of COVID-19 disease was aggravated by excessive consumption of animal proteins. The number of reported cases of SARS-CoV-2 virus (RPr) infection and deaths (IFR) from COVID-19 disease per one thousand inhabitants was significantly lower in regions with consumed predominantly plant-based foods minimal in EAAs. A positive relationship was found between the pathogenicity of SARS-CoV-2 and the amount of animal proteins ingested, with correlation coefficients r = 0.83 for RPr and r = 0.61 for IFR. Human coronaviruses contain much more EAAs than cellular organisms. Edible plant proteins contain 2-3 times less leucine, lysine, and especially threonine and valine (LKTV) than SARS-CoV-2 polypeptides. Optimal synthesis of the SARS-CoV-2 virus Pp1a polyprotein requires timely a huge amount of these free four EAAs. Conclusions. The limit of EAAs in food can be a competitive metabolic factor that reduces or inhibits the rate of intracellular synthesis of nonstructural polyproteins of SARS-CoV-2 virions. Deficiency of EAAs, especially free valine and threonine, can suppress the early translation of SARS-CoV-2 virus proteins. A diet low in EAAs and especially LKTV may prevent rapid, highly productive viral replication and pathogenic development of COVID-19.
ИЗОЛЯЦИЯ И ИЗУЧЕНИЕ IN VITRO БИОЛОГИЧЕСКИХ СВОЙСТВ ШТАММОВ КОРОНАВИРУСА SARS-COV-2, ОТНОСЯЩИХСЯ К РАЗЛИЧНЫМ ГЕНЕТИЧЕСКИМ ВАРИАНТАМ
Authors: Зайковская Анна , Гладышева Анастасия , Карташов Михаил , Таранов Олег , Овчинникова Алена , Шиповалов Андрей , Пьянков Олег
Published: February 2022

Цель. Изучить особенности репродукции штаммов коронавируса SARS-CoV-2 различных генетических линий в культуре клеток Vero E6. Материалы и методы. Штаммы коронавируса SARS-CoV-2 были взяты из Государственной коллекции возбудителей вирусных инфекций и риккетсиозов ФБУН ГНЦ БВ «Вектор» Роспотребнадзора. В работе использовали культуры клеток Vero и Vero E6. Кинетику накопления инфекционного вируса определяли путем титрования образцов культуральной жидкости через 24, 48, 72, 96 часов после инфицирования (MOI = 1 до 0,00001 ТЦД50/клетку). Образование бляшек изучали на культуре клеток Vero E6 под 0,2% агаровым покрытием. Анализ изображения и подсчёт размеров бляшек проводили в программе Adobe Photoshop CS6 Extended 13.0.1.3. Результаты. Описана динамика накопления инфекционного вируса в культуральной жидкости в зависимости от множественности инфицирования для штаммов коронавируса SARS-CoV-2, относящихся к разным генетическим линиям. Показаны различия морфологии бляшек для изученных штаммов. Штаммы коронавируса SARS-CoV-2, относящиеся к Alfa и Delta VOC нарабатываются в тирах более 7 lg ТЦД50/100мкл, что превышает значения для других штаммов. Генетический вариант Omicron VOC, имеющий наибольшее количество мутаций, под агаровым покрытием образует самые мелкие бляшки и при низкой множественности инфицирования имеет низкий уровень репродукции. Выводы. Штаммы коронавируса SARS-CoV-2, относящиеся к разным генетическим линиям, имеют существенные отличия в скорости репродукции в культуре клеток Vero Е6.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 12.02.2022 г. по 18.02.2022 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: February 2022

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19, за неделю с 12.02.2022 г. по 18.02.2022 г.
"Pero si el dedo de vn gigante”: En pos de la vida y obra de Juan Pérez de Menacho (1565-1626), primer teólogo de San Marcos y paradigma de la escolástica indiana
Authors: Sánchez Martínez César Félix
Published: February 2022

Juan Pérez de Menacho (1565-1626), teólogo y filósofo limeño de la Compañía de Jesús, inaugura la tradición escolástica en el Virreinato del Perú. La figura de Pérez de Menacho, catedrático de Prima en San Marcos, ha adoptado ribetes legendarios, especialmente luego de la posible pérdida de sus manuscritos en el incendio de la Biblioteca Nacional del Perú en 1943: valorado como una de las mentes más prodigiosas de su tiempo pero a la vez largamente ignorado o, incluso, mistificado. En este ensayo bio-bibliográfico, fragmento de una investigación mayor, se analizarán las escasas pero contrapuestas lecturas de su obra, se investigará el destino de sus manuscritos – que, como se verá, no se encuentran perdidos en su totalidad – y, a partir de sus casi desconocidos datos biográficos ofrecidos por un biógrafo barroco, se reconstruirá la vida y trayectoria de Pérez de Menacho, presentándolo como una figura paradigmática del ejercicio escolástico indiano.
Shortest Path Computation Technique in WSN for IOT Applications
Authors: Gill Ramandeep , Dubey Tarun Kumar
Published: February 2022

A Wireless Sensor Network (WSN) consists of a large no of sensor nodes deployed inside a physical environment that are capable of sensing and processing the data related to that environment and communicating it over the network. One of the vital applications of WSN is its suitability to smart cities that consist of many features such as smart buildings, smart education, smart governance, smart security, and smart mobility. This paper focuses on calculating the shortest path from the source node to the destination node in WSN by means of Dijkstra’s Algorithm. Various other algorithms are also compared and their suitability to different application scenarios is discussed. Simulations have been made for the smart city and Internet of Things (IoT) application by using the WSN simulator CupCarbon U-One 4.2.
Reference Genome Sequencing Of The Elite Bread Wheat Cultivar, “Sonmez”
Authors: Akpinar B. Ani , Leroy Philippe , Watson-Haigh Nathan S. , Baumann Ute , Barbe Valerie , Budak Hikmet
Published: February 2022

Objectives High-yielding crop varieties will become critical in meeting the future food demand in the face of worsening weather extremes and threatening biotic stressors. Bread wheat cultivar Sonmez-2001 is a registered variety that is notable for its performance under low irrigation conditions, which further improves upon irrigation. In order to explore and exploit the genomic potential of this elite variety, in 2015, we sequenced the modern elite cultivar Sonmez genome which represents a diverse genetic background. Data description Here, we provide a reference-guided whole genome sequence of Sonmez-2001, assembled into 21 chromosomes of the A, B and D genomes and totaling to 13.3 gigabase-pairs in length. Additionally, a de novo assembly, of an additional 1.05 gigabase-pairs, was generated that represents either Sonmez-specific sequences or sequences that considerably diverged between Sonmez and Chinese Spring. We identified up to 24 million sequence variants, of which up to 2.4% reside in coding sequences, that can be used to develop molecular markers that should be of immediate use to the cereal community.
Predicting of Agro-environmental Footprint with Artificial Intelligence (Soybean cultivation in various scenarios)
Authors: Kashka Faezeh Mohammadi , Sarvestani Zeinolabedin Tahmasebi , Pirdashti Hemmatollah , Motevali Ali , Nadi Mehdi
Published: February 2022

This study employed two artificial intelligence (AI) methods called the ANFIS–FCM algorithm as a novel computational method and an artificial neural network (ANN) as a conventional computational method in order to predict the environmental impacts of soybean production in different scenarios (i.e., soybean cultivation after rapeseed (R-S), wheat (W-S), and fallow (F-S)). The 175 data of life cycle assessment (LCA) method were collected from soybean farms. The two methods called the adaptive neuro-fuzzy inference based on fuzzy C-means clustering algorithm (FCM) and the artificial neural network (ANN) were adopted to predict environmental parameters. For this purpose, the life cycle of soybean production was assessed in terms of environmental impacts through the IMPACT2002+ method in SimaPro. According to the results, the production of each ton of soybean in the defined scenarios resulted in 0.0009 to 0.0016 DALY, 5476.18 to 8799.80 MJ primary, 1033.68 to 1840.70 PDF×m2×yr, and 563.55 to 880.61 kg CO2-eq damage to human health, resources, ecosystem quality, and climate change, respectively. Moreover, the weighted analysis indicated that various soybean production scenarios led to 293.87–503.73 mPt damage to the environment in which the R-S scenario had the best environmental performance. Notably, the emissions caused by the production and application of the diesel fuel followed by chemical fertilizers, particularly N and P fertilizers, were recognized as the most important environmental hotspots in soybean production. According to the results, the ANFIS–FCM algorithm acted as the best prediction model of environmental indicators for soybean cultivation in all cases related to the ANN. The RMSE and MAPE values obtained from ANFIS–FCM were lower than the values obtained from the ANN model for all environmental indicators. For the ANFIS–FCM and ANN algorithms, R2 ranged between 0.9967 to 0.9989 and 0.9269 to 0.9870, respectively. It can be concluded that the proposed ANFIS–FCM model is an efficient technique for obtaining accurate environmental prediction parameters of soybean cultivation.
Body Composition Evaluated by Bioelectrical Impedance Analysis in Patients With Pemphigus Vulgaris
Authors: Nasimi Maryam , Abedini Robabeh , Najafi Mohammad Taghi , Teymourpour Amir , Tootoonchi Nasim
Published: February 2022

Background:Pemphigus is a rare autoimmune disease affecting the skin and mucosa. Metabolic dysfunctions are shown to be associated with this disorder. The pemphigus disease area index (PDAI) score has been used as an accurate tool to assess the severity of pemphigus. This study aimed to evaluate the association between nutritional, metabolic indexes and Phase angle (PhA) in patients with pemphigus vulgaris (PV) and severity of disease based on the PDAI index.Methods:In this regard, 103 pemphigus patients and 125 healthy cases who were matched regarding their sex, age and body mass index were chosen. Results:Our results showed that there was a significant correlation between phase angle and sarcopenic index in patients with PV. Moreover, steroid dose had a significant correlation with sarcopenia index; while had no correlation with visceral fat area (VFA), BMI, volume load, percent body fat (PBF), and PhA. The volume load was lower but phase angle and sarcopenic index were higher in males, and females had a significantly higher BMI. Moreover, PBF and VFA were significantly higher in females compared to males. Besides, patients treated with rituximab had lower PBF and higher sarcopenic index and volume loads compared to patients receiving other adjuvant treatments. In comparison to healthy controls, PV patients had a significantly higher VFA and PBF.Conclusion:Conclusively, patients with PV had a significantly higher VFA and PBF compared to healthy objects. Moreover, sarcopenic index was related to steroid dose and history of rituximab treatment.
Identification of SARS-CoV-2 Variants Using Viral Sequencing for the Centers for Disease Control and Prevention Genomic U.S. Surveillance Program.
Authors: Goswami Chirayu , Sheldon Michael , Bixby Christian , Keddache Mehdi , Bogdanowicz Alexander , Wang Yihe , Schultz Jonathan , McDevitt Jessica , LaPorta James , Kwon Elaine , Buyske Steven , Garbolino Dana , Biloholowski Glenys , Pastuszak Alex , Storella Mary , Bhalla Amit , Charlier-Rodriguez Florence , Hager Russ , Grimwood Robin , Nahas Shareef Amin
Published: February 2022

Background
The Centers for Disease Control and Prevention contracted with laboratories to sequence the SARS-CoV-2 genome from positive samples across the United States to enable public health officials to investigate the impact of variants on disease severity as well as the effectiveness of vaccines and treatment. Herein we present the initial results from full SARS- CoV-2 viral genomic sequencing of 24,441 positive patient samples between April and June 2021.
Methods
RT-PCR confirmed (N Gene Ct value <30) positive patient samples, with nucleic acid extracted from saliva, nasopharyngeal and oropharyngeal swabs were selected for viral whole genome SARS-CoV-2 sequencing. Sequencing was performed using Illumina COVIDSeqtm protocol on either the NextSeq550 or NovaSeq6000 systems. Informatic variant calling, and lineage analysis were performed using DRAGEN COVID Lineage applications on Illumina’s Basespace cloud analytical system. All sequence data and variant calls were uploaded to NCBI and GISAID.
Results
An association was observed between higher sequencing coverage, quality, and samples with a lower Ct value, with <27 being optimal, across both sequencing platforms and sample collection methods. Both nasopharyngeal swabs and saliva samples were found to be optimal samples of choice for SARS-CoV2 surveillance sequencing studies, both in terms of strain identification and sequencing depth of coverage, with NovaSeq 6000 providing higher coverage than the NextSeq 550. The most frequent variants identified were the B.1.617.2 Delta (India) and P.1 Gamma (Brazil) variants.
Conclusion
These initial analyses highlight the importance of sequencing platform, sample collection methods, and RT-PCR Ct values in guiding surveillance efforts to inform the public of variant transmission rates, with the potential to facilitate correlation of sequencing data with vaccine efficacy rate and efficacy of infection prevention precautions.
The good, the bad, and the blameless: A thematic analysis of the parental role in childhood obesity on an Internet discussion board
Authors: Koivumäki Terhi , Jallinoja Piia
Published: February 2022

Background: Childhood obesity is an ongoing concern affecting many parents. Discussions around childhood obesity take place increasingly on social media, such as Internet discussion forums. On these platforms, parents present their experiences and apply cultural conceptions of childhood obesity. Argumentation on the platforms may affect how parents of children with obesity perceive obesity and adopt childhood obesity treatment. By exploring online discussions on childhood obesity, we can better understand the overall cultural conceptions concerning childhood obesity and the related responsibilities. This understanding may be used in health care to develop parents’ counselling for children’s weight issues. Method: We gathered and analysed 16 discussion threads on childhood obesity taken from a Finnish internet discussion forum, vauva.fi, between 2015 and 2021. In the analysis, we focused on threads where parents having a child with obesity took part. The data were analysed and interpreted with inductive thematic analysis.Results: In the online discussion on childhood obesity, the posts were mostly about the reasons and solutions for obesity, and obesity was discussed in the context of parental responsibility and lifestyle choices within the family. We located three themes that were used to define parenthood: being a good parent, being a bad parent, and being a blameless parent. Parents listed healthy elements in their family’s lifestyle to prove their good parenthood, whereas other commenters frequently tried to point out mistakes in the parents’ behaviour or offer advice. Moreover, many acknowledged that some factors causing childhood obesity were outside the parents’ influence. In addition, many parents were genuinely ignorant of the reasons for their child’s overweight. Conclusions: Our results showed that childhood obesity is not only a question of weight and lifestyle changes. Instead, defining parenthood and whether parents had fulfilled their tasks properly is the core issue. Consequently, counselling for parents in health care should be expanded from supporting a healthy lifestyle to strengthening parents’ identity as being good enough parents. Furthermore, efforts should be made to increase parents’ self-efficacy. In addition, supporting parents to accept and process feelings attached to the child’s weight would help them to manage in the present situation.
A randomized controlled trial evaluating the effect of Tai Chi on the drug craving in women
Authors: Wang Mu , Chen Yanyan , Zhang Xiaoyu , Li Huazhi , Yuan Cunfeng , Li Jin , Xu Yubing , Ding Zenghui , Ma Zuchang , Sun Yining
Published: February 2022

Purpose: This study was conducted in the purpose of investigating the effect of Tai Chi on drug craving for women with drug disorders.Methods: 112 women were recruited from a drug rehabilitation center in China, and 47 and 48 were finally analyzed in control group and exercise group, respectively. Exercise group underwent a three-month Tai Chi training, whereas the control group experienced no exercise intervention during the same time period. The drug craving was measured by visual analogue scale. In data analysis, repeated-measures were utilized to test the differences between the control and exercise group over the course of the experiment time.Results: The mean of the craving score significantly dropped from pre-test (control: mean=5.38, SD=3.04; exercise: mean=4.68, SD=2.93) to post-test (control: mean=4.03, SD=2.73; exercise: mean=1.91, SD=1.90) in both groups (control group: t=3.84, df=46, p<0.001; exercise group: t=5.941, df=47, p<0.001), with more decrease witnessed in the exercise group. Repeated- measures analysis with a Huynh-Feldt correction showed the significant effect of time (F=27.383, p<0.001) as well as the study group by time interaction (F=3.52, p=0.024). Conclusion: Tai Chi can ameliorate the drug craving in women and it could be a supportive treatment for drug addiction.
Responses of Natural Revegetation to Topographic Factors in Burned Areas
Authors: He Zhixue , Wang Lei , luo jun , Zhang Bin , Deng Qingchun , Liu Hui , Yang Haiqing
Published: February 2022

Background
Fire is an important disturbance of forest ecosystems and has important impacts on the subsequent succession of vegetation. To reveal the characteristics of natural understory revegetation and its response to environmental factors in burned areas, we studied a mountainous region with a subtropical climate (located in Xide County, China).
Results
Based on quadrat surveys and correlation analysis. the main results of this study were as follows. (1 ) Within four months after a fire, a total of 71 species, 52 genera, and 20 families of vegetation were found, representing significant increases from the first to second sampling periods. (2 ) The Shannon-Wiener, Simpson, and Margalef indexes increased between sampling periods, whereas the Pielou index decreased. The Margalef index had the most obvious change, increasing by 5.44 and 5.16 in lightly and severely burned areas, respectively. (3 ) The vegetation community stability in burned areas was low at the initial stage of revegetation. It tended to increase with the intensity of the fire that had affected a given sampling area. (4 ) Elevation and slope were the main physical factors affecting the vegetation biodiversity indexes and distribution. (5 ) The regrowth of understory vegetation was optimal in the gentle, shady slopes at low elevations.
Conclusions
This study elucidates the relationship between revegetation and topographic factors in burned areas and provides a scientific basis for guiding future ecological restoration.
Systematic Review and Meta-analysis of Ivermectin for Treatment of COVID-19: Evidence Beyond the Hype
Authors: Marcolino Milena Soriano , Meira Karina Cardoso , Guimarães Nathalia Sernizon , Motta Paula Perdigão , Chagas Victor Schulthais , Kelles Silvana Márcia Bruschi , Sá Laura Caetano , Valacio Reginaldo Aparecido , Ziegelmann Patrícia Klarmann
Published: February 2022

Background: The role of ivermectin in the treatment of COVID-19 is still under debate, yet the drug has been widely used in some parts of the world, as shown by impressive market data and ivermectin-related adverse event reports. The available body of evidence may have changed over the last months, as studies have been retracted and "standards of care" (SOC) used in control groups have changed with rapidly evolving knowledge on COVID-19 rapidly. This review aims to summarize and critically appraise the evidence of randomized controlled trials (RCTs) of ivermectin, assessing clinical outcomes in COVID-19 patients.Methods: RCTs evaluating the effects of ivermectin in adult patients with COVID-19 were searched through December 14, 2021, in four databases, L.OVE platform, clinical trial registries and pre-prints platforms. Primary endpoints included all-cause mortality and invasive ventilation requirement. Secondary endpoint was the occurrence of adverse events. Risk of bias was evaluated using the Cochrane Risk of Bias 2.0 tool. Random-effects were used to pool the risk ratios (RRs) of individual trials. The quality of evidence was evaluated using GRADE. Subgroup and sensitivity analysis were performed. The protocol was register in PROSPERO (CRD42021257471)Results: Twenty-one RCTs fulfilled inclusion criteria (n=2592). Of those, 12 compared ivermectin with placebo and in seven ivermectin associated with SOC was compared to SOC. Most RCTs had some concerns or high risk of bias, mostly due to lack of concealment of the randomization sequence and allocation, lack of blinding and high number of missing cases. Ivermectin did not show an effect in reducing mortality (RR=0.76; 95%CI:0.44 to 1.32) or mechanical ventilation (RR=0.83; 95%CI:0.31 to 2.22) in COVID-19 patients. This effect was consistent when comparing ivermectin vs. placebo, and ivermectin associated with SOC vs. SOC, as well as in sensitivity analysis. Additionally, there was very low quality of evidence regarding adverse effects (RR=0.95; 95%CI: 0.86 to 1.04).Conclusions: The evidence suggests that ivermectin does not reduce mortality risk and the risk of mechanical ventilation requirement. Although we did not observe an increase in the risk of adverse effects, the evidence is very uncertain regarding this endpoint.Funding: Research agencies FAPEMIG and CNPq.
Quality by Design Approach for Development and Validation of a RP-HPLC Method for Simultaneous Determination of Xipamide and Valsartan in Human Plasma
Authors: sebaiy mahmoud M. , El-Adl Sobhy M. , Baraka Mohamed M. , Hassan Amira A. , Elsayed Heba M.
Published: February 2022

A new rapid, simple and sensitive RP-HPLC method was developed through employing Quality by Design (QbD) approach for determination xipamide and valsartan in Human plasma. Fractional factorial design (FFD) was applied for screening of the four independent factors: pH, flow rate, detection wavelength, and % of MeOH. Analysis of variance (ANOVA) confirmed that flow rate and % of MeOH were only significant. The optimization of the chromatographic conditions was carried out through using central composite design (CCD). Method Analysis was achieved using BDS Hypersil C8 (250 × 4.6 mm, 5 µm) column and an isocratic mobile phase of MeOH and 0.05M KH2PO4 buffer pH 3 (64.5:35.5, v/v) at 1.2 mL/min flow rate with UV detection at 240nm and 10 µL injection volume. According to FDA guidelines, the method was then validated for the determination of the two drugs clinically in human plasma in respect of future pharmacokinetic and bioequivalence simulation studies. The standard curve was linear in the concentration range of 5-100 µg/mL for both drugs, with a correlation coefficients (R2) of 0.999. The average recoveries ranged from 99.89 to 100.03%. The proposed method showed good predictability and robustness.
Evaluation of Pulmonary Functions in Patients of Type 2 Diabetes Mellitus: a Cross-sectional Study
Authors: Mittal Swati , Jindal Manisha , Srivast Saurabh , Sinha Smriti
Published: February 2022

Background – Diabetes mellitus (DM) has been broadly recognized as syndrome of hyperglycemia leading to various macro and microvascular complications. The different physiological systems that have been identified as a target of these injurious effects of hyperglycemia are excretory system, ocular system, central nervous system and cardiovascular system. Till date not much focus has been given to respiratory system as a possible target for deleterious effect of hyperglycemia.Objective – To assess the pulmonary functions in subjects with type 2 diabetes mellitus (T2DM) and compare them with age and sex matched healthy controls. Methods – This study was conducted on one hundred and twenty-five patients of type 2 diabetes Mellitus and a comparative number of age and sex matched non-diabetic individuals (control group) who met the inclusion and exclusion criteria. RMS Helios 401 computerized spirometer was used to assess pulmonary functions.Result – Results showed significantly lower values of FVC, FEV1, FEF25-75% and MVV among diabetic subjects as compared to controls (<0.05).Conclusion – We found that pulmonary function parameters in diabetic subjects were consistently lower than in healthy controls.
MFOC-CliqueNet: A CliqueNet Based Optimal Combination of Multidimensional Features Classification Method for Large-Scale Laser Point Clouds
Authors: Wang Lei , Li Xiaonan , Zhang Zhiyong , He Yueshun
Published: February 2022

As large-scale laser 3D point clouds data contains massive and complex data, it faces great challenges in the automatic intelligent processing and classification of large-scale 3D point clouds. Aiming at the problem that 3D point clouds in complex scenes are self-occluded or occluded, which could affect the object classification accuracy, we propose a multi-dimensional feature optimization combination classification method named MFOC-CliqueNet based on CliqueNet for large-scale laser point clouds. The optimal combination matrix of multi-dimensional features is constructed by extracting the three-dimensional features and multi-directional two-dimensional features of three-dimensional point cloud. This is the first time that multi-dimensional optimal combination features are introduced into cyclic convolutional networks CliqueNet. It is important for large-scale three-dimensional point cloud classification. The experimental results show that the MFOC-CliqueNet framework can realize the latest level with fewer parameters. The classification accuracy of our method is 98.9% on the Large-Scale Scene Point Cloud Oakland dataset, which is better than other classification algorithms mentioned in this paper.
The Effects of Intermittent Fasting from Dawn to Sunset for 4 Consecutive Weeks on Spinal Cord Injury: A Randomized Controlled Trial
Authors: Xiang Wu , Wang Junyu , Ji Bingjin
Published: February 2022

Spinal cord injury (SCI) leads to the permanent loss or reduction of the function of movement and sensation, and no effective clinical treatment has been reported. Animal studies have shown that intermittent fasting (IF) has a neuroprotective effect on SCI, but it has not been used in clinical treatment of SCI. Intermittent fasting from dawn to sunset is a form of fasting during human activity, which may improve the dysfunction of patients with SCI. Therefore, this study is the first to explore the safety of intermittent fasting from dawn to sunset in the treatment of SCI. Twenty-six SCI participants were randomly divided into a control group or an IF group. The IF group was fasted for more than 14 hours a day, and the control group had normal diet and water. Changes in blood routine, liver function, renal function, electrolytes, plasma glucose, weight, body mass index (BMI), American Spinal Injury Association (ASIA) score and occurrence of adverse events were monitored before enrollment and at the end of IF intervention. The IF group developed hypoglycemia, hunger, weight loss, and intestinal dysfunction (nausea, abdominal discomfort, diarrhea). There were no significant differences in blood routine, liver function, renal function, electrolytes and ASIA scores before and after IF intervention, or compared with the control group. Compared with the control group, there was no difference in body weight and BMI before IF intervention, and weight loss after IF intervention, but there was no significant change in BMI. Moreover, there were significant differences in body weight and BMI before and after IF intervention. The plasma glucose of the IF group began to decrease from the 14th day, and there were significant differences in plasma glucose before and after IF intervention. These findings suggested that IF was safe and feasible for SCI participants.
Four novel genes associated with longevity found in Cane Corso purebred dogs
Authors: Korec Evžen , Ungrová Lenka , Hejnar Jiří , Grieblová Adéla
Published: February 2022

Background: Longevity-related genes have been found in several animal species as well as in humans. The goal of this study was to perform genetic analysis of long-lived Cane Corso dogs with the aim to find genes that are associated with longevity. Results: SNPs with particular nucleotides were significantly overrepresented in long-lived dogs in four genes, TDRP, MC2R, FBXO25 and FBXL21. In FBXL21, the longevity-associated SNP localises to the exon. In the FBXL21 protein, tryptophan in long-lived dogs replaced arginine present in reference dogs.Conclusions: Four SNPs associated with longevity in dogs were identified using GWAS. We conclude that genes TDRP, MC2R, FBXO25 and FBXL21 are associated with longevity in Cane Corso dogs.