bluuebunny/crossref_metadata_2025 huggingface dataset:
RNF6 activates TGF-β1/c-Myb pathway to promote EMT in esophageal squamous cell carcinoma
Authors: Cheng Jingge , Wu Kun , Yang Qian , Zhu Ziming , Zhao Hongye
Published: February 2023

Objective

This study aimed to investigate RING-Finger Protein 6 (RNF6) expression in esophageal squamous cell carcinoma (ESCC) cells and whether it affects cell proliferation, invasion, and migration by regulating the TGF-β1/c-Myb pathway.

Methods

TCGA database was used to analyze RNF6 expression in normal tissues and esophageal cancer tissues. Kaplan–Meier method was used to examine the correlation between RNF6 expression and patient prognosis. SiRNA interference vector and RNF6 overexpression plasmid were constructed, and RNF6 was transfected into Eca-109 and KYSE-150 esophageal cancer cell line. In vitro scratch assay and Transwell assay were conducted to investigate the effects of RNF6 on the migration and invasion of Eca-109 and KYSE-150 cells. RT-PCR detected the expression of Snail, E-cadherin, and N-cadherin, and TUNEL detected the apoptosis of cells.

Results

RNF6 up-regulation promoted the progression of esophageal cancer and predicted poor prognosis. RNF6 also enhanced the migration and invasion of ESCC cells in vitro. RNF6 silencing inhibited the migration and invasion of ESCC cells. TGF-β inhibitors reversed the oncogenic effects of RNF6. RNF6 regulated the migration and invasion of ESCC cells by activating the TGF-β pathway. RNF6/TGF-β1 promoted esophageal cancer progression through c-Myb.

Conclusion

RNF6 promotes the proliferation, invasion, and migration of ESCC cells possibly by activating the TGF-β1/c-Myb pathway and affects the progression of ESCC.
High-risk multiple myeloma predicted by circulating plasma cells and its genetic characteristics
Authors: Xia Yuan , Shen Na , Zhang Run , Wu Yujie , Shi Qinglin , Li Jianyong , Chen Lijuan , Xu Min , Jin Yuanyuan
Published: February 2023

Introduction

Circulating plasma cells (CPC) have been reported to be one of the indicators of high-risk multiple myeloma (MM), yet the prognostic significance of CPC in Chinese population and the genetic mechanisms underlying CPC formation have not been fully elucidated.

Methods

Patients with newly diagnosed MM were included in this study. We used multi-parameter flow cytometry (MFC) for CPC quantification and next-generation sequencing (NGS) technology for mutational landscape mapping to identify the correlation of CPC level with clinical characteristics and the mutations.

Results

A total of 301 patients were enrolled in this investigation. We demonstrated that CPC quantification could effectively mirror the tumor load, and CPC ≥ 0.105% at diagnosis or detectable CPC after therapy indicates poor treatment response and adverse outcome, and the introduction of CPC into the R-ISS enables a more accurate risk stratification. Interestingly, we noticed an elevated percentage of light-chain MM in patients with higher CPC. Mutational landscape revealed that patients harboring mutations in TP53, BRAF, DNMT3A, TENT5C, and IL-6/JAK/STAT3 pathway-related genes tended to have higher CPC levels. Gene enrichment analysis demonstrated that pathways involving chromosome regulation and adhesion may be potential mechanisms accounting for CPC formation.

Discussion

Accordingly, quantification of CPC may provide a less-invasive and reliable approach for identifying high-risk MM in Chinese population.
Prognostic significance of ING3 expression in patients with cancer: A systematic review and meta-analysis
Authors: Li Zehan , Xu Shengchao , Chen Lin , Huang Shuqi , Kuerban Xieyida , Li Tianyu
Published: February 2023

Background

It has been reported that ING3 inhibits the progression of various cancers. However, some studies have shown that it promotes the development of prostate cancer. The purpose of this study was to investigate whether ING3 expression is associated with the prognosis of patients with cancer.

Materials and methods

PubMed, Cochrane Database, Embase, Medline, ScienceDirect, Scopus and Web of Science were searched until September 2022. The hazard ratio (HR)/odds ratio (OR) and 95% confidence interval (95% CI) were calculated using Stata 17 software. We used the Newcastle-Ottawa Scale (NOS) to assess the risk of bias.

Result

Seven studies involving 2371 patients with five types of cancer were included. The results showed that high expression of ING3 was negatively associated with a more advanced TNM stage (III-IV vs. I-II) (OR=0.61, 95% CI: 0.43-0.86), lymph node metastasis (OR=0.67, 95% CI: 0.49-0.90) and disease-free survival (HR=0.63, 95% CI: 0.37-0.88). However, ING3 expression was not associated with overall survival (HR=0.77, 95% CI: 0.41-1.12), tumor size (OR=0.67, 95% CI: 0.33-1.37), tumor differentiation (OR=0.86, 95% CI: 0.36-2.09) and gender (OR=1.14, 95% CI: 0.78-1.66).

Conclusion

This study showed that the expression of ING3 was associated with better prognosis, suggesting that ING3 may be a potential biomarker for cancer prognosis.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier (CRD42022306354).
Neoadjuvant plus adjuvant combined or sequenced vemurafenib, cobimetinib and atezolizumab in patients with high-risk, resectable BRAF-mutated and wild-type melanoma: NEO-TIM, a phase II randomized non-comparative study
Authors: Ascierto Paolo A. , Cioli Eleonora , Chiarion-Sileni Vanna , Quaglino Pietro , Spagnolo Francesco , Guidoboni Massimo , Del Vecchio Michele , Peris Ketty , Queirolo Paola , Fioretto Luisa , Caracò Corrado , Paone Miriam , Sorrentino Antonio , Capone Mariaelena , Giannarelli Diana , Ferrara Gerardo , Massi Daniela , Trojaniello Claudia
Published: February 2023

Background

Following the increased survival of patients with metastatic melanoma thanks to immunotherapy and targeted therapy, neoadjuvant approaches are being investigated to address the unmet needs of unresponsive and intolerant patients. We aim to investigate the efficacy of neoadjuvant plus adjuvant combined or sequenced vemurafenib, cobimetinib and atezolizumab in patients with high-risk, resectable BRAF-mutated and wild-type melanoma.

Methods

The study is a phase II, open-label, randomized non-comparative trial in patients with stage IIIB/C/D surgically resectable, BRAF-mutated and wild-type melanoma, with three possible treatments: (1) vemurafenib 960 mg twice daily from day 1 to 42; (2) vemurafenib 720 mg twice daily from day 1 to 42; (3) cobimetinib 60 mg once daily from day 1 to 21 and from day 29 to 42; and (4) atezolizumab 840 mg for two cycles (day 22 and day 43).

Patients will be randomized to three different arms: A) BRAF-mutated patients will receive over 6 weeks (1) + (3); B) BRAF-mutated patients will receive over 6 weeks (2) + (3) + (4); C) BRAF wild-type patients will receive over 6 weeks (3) + (4). All patients will also receive atezolizumab 1200 mg every 3 weeks for 17 cycles after surgery and after a second screening period (up to 6 weeks).

Discussion

Neoadjuvant therapy for regional metastases may improve operability and outcomes and facilitate the identification of biomarkers that can guide further lines of treatment. Patients with clinical stage III melanoma may especially benefit from neoadjuvant treatment, as the outcomes of surgery alone are very poor. It is expected that the combination of neoadjuvant and adjuvant treatment may reduce the incidence of relapse and improve survival.

Clinical trial registration

eudract.ema.europa.eu/protocol.htm, identifier 2018-004841-17.
Characteristics of immunological events in Epstein-Barr virus infection in children with infectious mononucleosis
Authors: Zhang Yunyun , Huang Chengrong , Zhang Hao , Duan Zhi , Liu Qian , Li Jianfei , Zong Qiyin , Wei Yu , Liu Futing , Duan Wanlu , Chen Liwen , Zhou Qiang , Wang Qin
Published: February 2023

Backgrounds & aims

Epstein-Barr virus (EBV) infection occurs commonly in children and may cause acute infectious mononucleosis (AIM) and various malignant diseases. Host immune responses are key players in the resistance to EBV infection. We here assessed the immunological events and laboratory indicators of EBV infection, as well as determined the clinical usefulness of evaluating the severity and efficacy of antiviral therapy in AIM patients.

Methods

We enrolled 88 children with EBV infection. The immune environment was defined by immunological events such as frequencies of lymphocyte subsets, phenotypes of T cells, and their ability to secrete cytokines, and so on. This environment was analyzed in EBV-infected children with different viral loads and in children in different phases of infectious mononucleosis (IM) from disease onset to convalescence.

Results

Children with AIM had higher frequencies of CD3+ T and CD8+ T cells, but lower frequencies of CD4+ T cells and CD19+ B cells. In these children, the expression of CD62L was lower and that of CTLA-4 and PD-1 was higher on T cells. EBV exposure induced granzyme B expression, but reduced IFN-γ secretion, by CD8+ T cells, whereas NK cells exhibited reduced granzyme B expression and increased IFN-γ secretion. The frequency of CD8+ T cells was positively correlated with the EBV DNA load, whereas the frequencies of CD4+ T cells and B cells were negatively correlated. During the convalescent phase of IM, CD8+ T cell frequency and CD62L expression on T cells were restored. Moreover, patient serum levels of IL-4, IL-6, IL-10, and IFN-γ were considerably lower throughout the convalescent phase than throughout the acute phase.

Conclusion

Robust expansion of CD8+ T cells, accompanied by CD62L downregulation, PD-1 and CTLA-4 upregulation on T cells, enhanced granzyme B production, and impaired IFN-γ secretion, is a typical characteristic of immunological events in children with AIM. Noncytolytic and cytolytic effector functions of CD8+ T cells are regulated in an oscillatory manner. Furthermore, the AST level, number of CD8+ T cells, and CD62L expression on T cells may act as markers related to IM severity and the effectiveness of antiviral treatment.
Methodological, reporting, and evidence quality of systematic reviews of traditional Chinese medicine for ischemic stroke
Authors: Sun Shouyuan , Zhao Liang , Zhou Xiaoli , Liu Xuewu , Xie Zongzhi , Ren Jun , Zhou Baoyuan , Pan Yawen
Published: February 2023

Objective: The aim of this study is to critically appraise whether published systematic reviews/meta-analyses of traditional Chinese medicine for adults with ischemic stroke are of sufficient quality and to rate the quality of evidence using the Grading of Recommendations, Assessment, Development, and Evaluation approach.

Method: A literature search was performed in the Cochrane Library, PubMed, Chinese National Knowledge Infrastructure, and SinoMed databases by March 2022. The inclusion criteria were systematic reviews/meta-analyses of traditional Chinese medicine in adults who suffered from ischemic stroke. A Measurement Tool to Access Systematic Reviews 2 (AMSTAR-2) and Preferred Reporting Items for Systematic Reviews and Meta-Analyses for Abstract (PRISMA-A) statements were used to assess the methodological and reporting quality of the included reviews. The Grading of Recommendations, Assessment, Development, and Evaluation system was utilized to assess each report’s evidence level.

Results: Of the 1,908 titles and abstracts, 83 reviews met the inclusion criteria. These studies were published between 2005 and 2022. The results of AMSTAR-2 showed that 51.4% of the items were reported, but the registration, reasons for the inclusion of study design, the list of excluded studies, and funding information were ignored in the majority of the reviews. The results of PRISMA-A showed that 33.9% of items were reported, and the information on registration, limitation, and funding was not available in many publications. The assessment of the evidence with the Grading of Recommendations, Assessment, Development, and Evaluation showed that more than half (52/83) of the included studies had either low or very low levels of evidence.

Conclusion: The reporting quality in the abstract of systematic reviews/meta-analyses on traditional Chinese medicine for ischemic stroke is poor and does not facilitate timely access to valid information for clinical practitioners. Although the methodological quality is of a medium level, this evidence lacks certainty, especially with a high risk of bias in individual studies.
Development and application of automatic monitoring equipment for differential deformation of element joint in immersed tunnel
Authors: Guo Hongyan , Yan Yu , Ding Hao , Liu Xinrong , Yang Men
Published: February 2023

The reliability of the immersed tunnel element joint is the key to determine whether the immersed tunnel can operate safely. At present, the immersed tunnel monitoring mostly pays attention to the joint opening and closing amount and neglects the differential deformation of the joint. Based on the immersed tunnel of Hong Kong-Zhuhai-Macao Bridge, combined with the operating environment and structural characteristics of the immersed tunnel, this paper introduces a close-range photogrammetry method to monitor the differential deformation of the immersed tunnel element joint. Through theoretical analysis, software and hardware development, laboratory test and field test, the paper puts forward puts forward a comprehensive multi-parameter evaluation and screening algorithm of boundary fitting ellipse based on fitting rate, ellipticity and area difference and a micro-displacement correction algorithm for camera based on three-dimensional calibration object, and develops an automatic monitoring system equipment for differential deformation of immersed tunnel element joint. Upon tests in tunnels, the monitoring equipment is proven in automatic monitoring on differential deformation of immersed tunnel element joints. This equipment has been successfully applied to the E31~E32 element joint of Hong Kong-Zhuhai-Macao Bridge immersed tunnel, which verifies the effectiveness of the equipment from the perspective of practical engineering application.
Exposure to artificial light at night mediates the locomotion activity and oviposition capacity of Dastarcus helophoroides (Fairmaire)
Authors: Jiang Xiang-lan , Ren Zhe , Hai Xiao-xia , Zhang Ling , Wang Zhi-gang , Lyu Fei
Published: February 2023

Light entrains the endogenous circadian clocks of organisms to synchronize their behavioral and physiological rhythms with the natural photoperiod. The presence of artificial light at night disrupts these photoperiodic cues and is currently considered to be a major threat to key fitness-related behaviors, including sleep disruption and physiological stress. Research on the ecological influence of forest pest and their natural enemies is lacking. The wood-boring insects significantly damage forest and urban forest ecosystem functions. The parasitic beetles, Dastarcus helophoroides is an important natural enemy of wood-boring insects, especially those in the Cerambycidae family. However, the effect of artificial light at night on the locomotor rhythms and oviposition capacity of D. helophoroides has received little attention. To address this gap, diel changes in the locomotor activity and number of eggs laid by female D. helophoroides was analyzed under different light-dark (LD) cycles and temperatures. The results showed that the 24-h rhythmic of locomotor activity in these beetles was elevated in darkness and reduced under illumination, indicating that they are nocturnal insects. This activity has two major peaks, the evening (1–8 h after lights off) and morning (3.5–12.5 h after lights off) components, reflecting that light mediate regular changes in locomotor activity. Moreover, the circadian rhythms and active percentage were influenced by the illumination duration and temperature, especially constant light and 40°C. Females laid more eggs under the 16 L: 8 D cycles at 30°C than under the other combinations of photoperiod (including constant light and darkness) and temperature. Finally, the potential influence of exposure to four ecologically relevant intensities of artificial light at night (0, 1, 10 or 100 lx) on oviposition capacity was studied. The results showed that lifetime exposure to bright artificial light (1–100 lx) at night decreased the number of eggs laid relative to those laid with no lighting at night. These results demonstrate that chronic exposure to bright artificial light at night may influence the locomotor activity and oviposition capacity of this parasitic beetle.
Uncovering the genetic links of diabetic erectile dysfunction and chronic prostatitis/chronic pelvic pain syndrome
Authors: Yuan Penghui , Sun Taotao , Han Zhengyang , Chen Yinwei , Meng Qingjun
Published: February 2023

Background: Clinical associations between erectile dysfunction and chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) have been noticed, but the common pathogenic mechanisms between them remain elusive. The aim of the study was to mine shared genetic alterations between ED and chronic prostatitis/chronic pelvic pain syndrome.

Method: Transcriptome data of ED and chronic prostatitis/chronic pelvic pain syndrome-related genes (CPRGs) were retrieved from relevant databases and differentially expressed analysis was used to obtain significant CPRGs. Then function enrichment and interaction analyses were performed to show shared transcriptional signature, including gene ontology and pathway enrichment, the construction of protein-protein interaction (PPI) network, cluster analysis, and co-expression analysis. Hub CPRGs and key cross-link were selected by validating these genes in clinical samples, chronic prostatitis/chronic pelvic pain syndrome and ED-related datasets. Then the miRNA-OSRGs co-regulatory network was predicted and validated. Subpopulation distribution and disease association of hub CPRGs were further identified.

Result: Differentially expressed analysis revealed 363 significant CPRGs between ED and chronic prostatitis/chronic pelvic pain syndrome, functioning in inflammatory reaction, oxidative stress, apoptosis, smooth muscle cell proliferation, and extracellular matrix organization. A PPI network containing 245 nodes and 504 interactions was constructed. Module analysis depicted that multicellular organismal process and immune metabolic process were enriched. 17 genes were screened in PPI via topological algorithms, and reactive oxygen species as well as interleukin-1 metabolism were regarded as the bridging interactive mechanism. After screening and validation, a hub-CPRG signature consisting of COL1A1, MAPK6, LPL, NFE2L2 and NQO1 were identified and associated miRNA were verified. These miRNAs played an important role in immune and inflammatory response likewise. Finally, NQO1 was identified as a key genetic link between ED and chronic prostatitis/chronic pelvic pain syndrome. It was predominately enriched in corpus cavernosum endothelial cell, and correlated with other male urogenital and immune system diseases tightly.

Conclusion: We identified the genetic profiles as well as corresponding regulatory network underlying interaction between ED and chronic prostatitis/chronic pelvic pain syndrome via multi-omics analysis. These findings expanded a new understanding for the molecular mechanism of ED with chronic prostatitis/chronic pelvic pain syndrome.
Heart rate variability as a predictor of stroke course, functional outcome, and medical complications: A systematic review
Authors: Aftyka Joanna , Staszewski Jacek , Dębiec Aleksander , Pogoda-Wesołowska Aleksandra , Żebrowski Jan
Published: February 2023

Background: Heart rate variability (HRV) is a non-invasive marker of autonomic nervous system function that is based on the analysis of length differences between subsequent RR intervals of the electrocardiogram. The aim of this systematic review was to assess the current knowledge gap in the utility of HRV parameters and their value as predictors of the acute stroke course.

Methods: A systematic review was performed in accordance with the PRISMA guidelines. Relevant articles published between 1 January 2016 and 1 November 2022 available in the PubMed, Web of Science, Scopus, and Cochrane Library databases were obtained using a systematic search strategy. The following keywords were used to screen the publications: “heart rate variability” AND/OR “HRV” AND “stroke.” The eligibility criteria that clearly identified and described outcomes and outlined restrictions on HRV measurement were pre-established by the authors. Articles assessing the relationship between HRV measured in the acute phase of stroke and at least one stroke outcome were considered. The observation period did not exceed 12 months. Studies that included patients with medical conditions influencing HRV with no established stroke etiology and non-human subjects were excluded from the analysis. To minimize the risk of bias, disagreements throughout the search and analysis were resolved by two independent supervisors.

Results: Of the 1,305 records obtained from the systematic search based on keywords, 36 were included in the final review. These publications provided insight into the usability of linear and non-linear HRV analysis in predicting the course, complications, and mortality of stroke. Furthermore, some modern techniques, such as HRV biofeedback, for the improvement of cognition performance after a stroke are discussed.

Discussion: The present study showed that HRV could be considered a promising biomarker of a stroke outcome and its complications. However, further research is needed to establish a methodology for appropriate quantification and interpretation of HRV-derived parameters.
Nectary development in Cleome violacea
Authors: Carey Shane , Zenchyzen Brandi , Deneka A. J. , Hall Jocelyn C.
Published: February 2023

Nectaries are a promising frontier for plant evo-devo research, and are particularly fascinating given their diversity in form, position, and secretion methods across angiosperms. Emerging model systems permit investigations of the molecular basis for nectary development and nectar secretion across a range of taxa, which addresses fundamental questions about underlying parallelisms and convergence. Herein, we explore nectary development and nectar secretion in the emerging model taxa, Cleome violacea (Cleomaceae), which exhibits a prominent adaxial nectary. First, we characterized nectary anatomy and quantified nectar secretion to establish a foundation for quantitative and functional gene experiments. Next, we leveraged RNA-seq to establish gene expression profiles of nectaries across three key stages of development: pre-anthesis, anthesis, and post-fertilization. We then performed functional studies on five genes that were putatively involved in nectary and nectar formation: CvCRABSCLAW (CvCRC), CvAGAMOUS (CvAG), CvSHATTERPROOF (CvSHP), CvSWEET9, and a highly expressed but uncharacterized transcript. These experiments revealed a high degree of functional convergence to homologues from other core Eudicots, especially Arabidopsis. CvCRC, redundantly with CvAG and CvSHP, are required for nectary initiation. Concordantly, CvSWEET9 is essential for nectar formation and secretion, which indicates that the process is eccrine based in C. violacea. While demonstration of conservation is informative to our understanding of nectary evolution, questions remain. For example, it is unknown which genes are downstream of the developmental initiators CvCRC, CvAG, and CvSHP, or what role the TCP gene family plays in nectary initiation in this family. Further to this, we have initiated a characterization of associations between nectaries, yeast, and bacteria, but more research is required beyond establishing their presence. Cleome violacea is an excellent model for continued research into nectary development because of its conspicuous nectaries, short generation time, and close taxonomic distance to Arabidopsis.
Uncovering the role of wheat magnesium transporter family genes in abiotic responses
Authors: Tang Yanhong , Yang Xiaoyue , Li Han , Shuai Yating , Chen Wang , Ma Dongfang , Lü Zhichuang
Published: February 2023

Background

The CorA / MGT / MRS2 family proteins are an important group of magnesium transporter proteins that maintain magnesium ion homeostasis in plant cells. However, little is known about the MGT functions in wheat.

Methods

The known MGT sequences were used as queries to BlastP against wheat genome IWGSC RefSeq v2.1 assembly (E-value <10–5). Chromosome localization information for each TaMGT gene was obtained from the GFF3 file of the wheat genome data (IWGSCv2.1).The sequence of 1500 bp upstream of the TaMGT genes was extracted from the wheat genome data. The cis-elements were analyzed using PlantCARE online tool.

Result

A total of 24 MGT genes were identified on 18 chromosomes of wheat. After functional domain analysis, only TaMGT1A, TaMGT1B, and TaMGT1D had GMN mutations to AMN, while all the other genes had conserved GMN tripeptide motifs. Expression profiling showed that the TaMGT genes were differentially expressed under different stresses and at different growth and development stages. The expression levels of TaMGT4B and TaMGT4A were significantly up-regulated in cold damage. In addition, qRT-PCR results also confirmed that these TaMGT genes are involved in the wheat abiotic stress responses.

Conclusion

In conclusion, The results of our research provide a theoretical basis for further research on the function of TaMGT gene family in wheat.
Strigolactones modulate stem length and diameter of cherry rootstocks through interaction with other hormone signaling pathways
Authors: Liu Xunju , Xu Yan , Sun Wanxia , Wang Jiyuan , Gao Yixin , Wang Lei , Xu Wenping , Wang Shiping , Jiu Songtao , Zhang Caixi
Published: February 2023

Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our studies showed that the synthetic SL analog rac-GR24 and the biosynthetic inhibitor TIS108 affected stem length and diameter, aboveground weight, and chlorophyll content. The stem length of cherry rootstocks following TIS108 treatment reached a maximum value of 6.97 cm, which was much higher than that following rac-GR24 treatments at 30 days after treatment. Stem paraffin section showed that SLs affected cell size. A total of 1936, 743, and 1656 differentially expressed genes (DEGs) were observed in stems treated with 10 μM rac-GR24, 0.1 μM rac-GR24, and 10 μM TIS108, respectively. RNA-seq results highlighted several DEGs, including CKX, LOG, YUCCA, AUX, and EXP, which play vital roles in stem growth and development. UPLC-3Q-MS analysis revealed that SL analogs and inhibitors affected the levels of several hormones in the stems. The endogenous GA3 content of stems increased significantly with 0.1 μM rac-GR24 or 10 μM TIS108 treatment, which is consistent with changes in the stem length following the same treatments. This study demonstrated that SLs affected stem growth of cherry rootstocks by changing other endogenous hormone levels. These results provide a solid theoretical basis for using SLs to modulate plant height and achieve sweet cherry dwarfing and high-density cultivation.
Grapevine bZIP transcription factor bZIP45 regulates VvANN1 and confers drought tolerance in Arabidopsis
Authors: Niu Shuaike , Gu Xiangyang , Zhang Qian , Tian Xuemin , Chen Zhan , Liu Jingru , Wei Xiaoju , Yan Chengxiang , Liu Ziwen , Wang Xiaoji , Zhu Zhengge
Published: February 2023

Drought is a severe environmental condition that restricts the vegetative growth and reduces the yield of grapevine (Vitis vinifera L.). However, the mechanisms underlying grapevine response and adaptation to drought stress remain unclear. In the present study, we characterized an ANNEXIN gene, VvANN1, which plays a positive role in the drought stress response. The results indicated that VvANN1 was significantly induced by osmotic stress. Expression of VvANN1 in Arabidopsis thaliana enhanced osmotic and drought tolerance through modulating the level of MDA, H2O2, and O2·- at the seedling stage, implying that VvANN1 might be involved in the process of ROS homeostasis under drought or osmotic stress conditions. Moreover, we used yeast one-hybridization and chromatin immunoprecipitation assays to show that VvbZIP45 could regulate VvANN1 expression by directly binding to the promoter region of VvANN1 in response to drought stress. We also generated transgenic Arabidopsis that constitutively expressed the VvbZIP45 gene (35S::VvbZIP45) and further produced VvANN1Pro::GUS/35S::VvbZIP45 Arabidopsis plants via crossing. The genetic analysis results subsequently indicated that VvbZIP45 could enhance GUS expression in vivo under drought stress. Our findings suggest that VvbZIP45 may modulate VvANN1 expression in response to drought stress and reduce the impact of drought on fruit quality and yield.
The relationship between social support and Internet addiction among Chinese college freshmen: A mediated moderation model
Authors: Lu Xiaoman , Zhang Mengnan , Zhang Jingqiu
Published: February 2023

Purpose

Internet addiction has become a worldwide mental health problem, and this problem is particularly prominent in China. Although current studies have shown that social support is closely related to Internet addiction, the mechanism of the relationship between the two is not clear at present. This study aimed to find out the influencing factors and the mechanism of Internet addiction among college freshmen, and to form scientific prevention and intervention plan on this basis.

Method

This study adopts the cluster sampling method to select 322 college freshmen in a typical postsecondary school in Shandong Province, using Chinese Internet Addiction Scale (CIAS), Social Support Rating Scale (SSRS), and Network-related Maladaptive Cognition Scale (NRMCS) to investigate the relationship between social support, network-related maladaptive cognition, gender, and the degree of Internet addiction.

Results

The findings of this study are as follows: (1) After controlling age and family location, social support had a significant negative predictive effect on Internet addiction; (2) Gender acted as a moderator between the relationship of social support and Internet addiction; and (3) Additionally, the moderating effect of gender was completely mediated by network-related maladaptive cognition.

Conclusion

There is a mediated moderating effect between social support and Internet addiction. That is, gender plays a moderating role between social support and Internet addiction, and this moderating effect is mediated by network maladaptive cognition.
Implementation of open dialogue in Germany: Efforts, challenges, and obstacles
Authors: Heumann Kolja , Kuhlmann Mira , Böning Maike , Tülsner Helene , Pocobello Raffaella , Ignatyev Yuriy , Aderhold Volkmar , von Peter Sebastian
Published: February 2023

Purpose

The Open Dialogue (OD) approach has been implemented in different countries worldwide. OD not only depends on therapeutic principles but also requires a distinct set of structural changes that may impede its full implementation. In Germany, OD is currently practiced in different mental health care settings across the country. Yet, full implementation of OD principles is limited due to the extreme structural and financial fragmentation of the German mental health care system. With this as a background, the aim of this study was to investigate the efforts, challenges and obstacles of OD implementation in Germany.

Methods

This article presents the German results from the international HOPEnDIALOGUE survey, supplemented with expert interview data. Thirty eight teams currently providing OD took part in the survey. Sixteen expert interviews were carried out with stakeholders from various care settings. Survey data were analyzed descriptively and the qualitative data were evaluated using a thematic analysis approach.

Results

While having to adapt to the fragmented German health care system, OD has been mainly implemented from outpatient service providers and stand-alone services. About half of the teams implemented OD under the conditions of cross-sectoral model contracts and, thus, are considerably limited when it comes to OD implementation. Altogether, OD is not implemented to its full extent in each of the institutions surveyed. Similarly, the expert interviews revealed various challenges that mainly relate to the realization of OD’s structural principles, whereas the implementation of its therapeutic benefits remains less affected. However, these challenges have managed to lead to great commitment by single teams and a certain level of implementation of OD-related concepts.

Conclusion

OD in Germany can currently only be fully implemented under the cross-sectoral care model contract system that is often temporary, thus significantly hindering its continuous development. Any evaluation of OD’s effectiveness in Germany thus needs to take into account the fragmented nature of the country’s health care system and control for the multiple barriers that impede implementation. Reforms of the German health care system are also urgently needed to create more favorable conditions for the implementation of OD.
Eristic reasoning: Adaptation to extreme uncertainty
Authors: Kurdoglu Rasim Serdar , Jekel Marc , Ateş Nüfer Yasin
Published: February 2023

Heuristics (shortcut solution rules) can help adaptation to uncertainty by leading to sufficiently accurate decisions with little information. However, heuristics would fail under extreme uncertainty where information is so scarce that any heuristic would be highly misleading for accuracy-seeking. Thus, under very high levels of uncertainty, decision-makers rely on heuristics to no avail. We posit that eristic reasoning (i.e., self-serving inferences for hedonic pursuits), rather than heuristic reasoning, is adaptive when uncertainty is extreme, as eristic reasoning produces instant hedonic gratifications helpful for coping. Eristic reasoning aims at hedonic gains (e.g., relief from the anxiety of uncertainty) that can be pursued by self-serving inferences. As such, eristic reasoning does not require any information about the environment as it instead gets cues introspectively from bodily signals informing what the organism hedonically needs as shaped by individual differences. We explain how decision-makers can benefit from heuristic vs. eristic reasoning under different levels of uncertainty. As a result, by integrating the outputs of formerly published empirical research and our conceptual discussions pertaining to eristic reasoning, we conceptually criticize the fast-and-frugal heuristics approach, which implies that heuristics are the only means of adapting to uncertainty.
The influence of music environment on conceptual design creativity
Authors: Xia Tiansheng , Sun Yongqing , An Yi , Li Linli
Published: February 2023

Introduction

Creativity plays an important role in design. However, there have been mixed results about whether music, as an environmental stimulus, improves design creativity performance.

Methods

Participants were 57 design major students who were randomly assigned to one of three groups, with 19 students in each group: no music, pure music, and music with intelligible semantic information (unrelated to the task) playing in the background. Each participant completed a design task (design a tool for storing painting materials), with two phases in it, one that involved idea generation (divergent thinking) and one that involved idea evaluation (convergent thinking). Performance in the two phases was rated based on six indices of creativity (fluency; flexibility; adaptability; feasibility; usefulness; novelty) and overall design creativity (ODC).

Results

The results of one-way ANOVAs with Bonferroni correction showed that neither music environment had a significant influence on divergent thinking in idea generation nor convergent thinking in idea evaluation. However, both music environments had a significantly positive effect on novelty and ODC.

Discussion

We discuss the implications of our current results for fostering designers’ creativity performance.
The spillover effect of work connectivity behaviors on employees' family: Based on the perspective of work-home resource model
Authors: He Hui , Li Dan , Zhou Yuanyuan , Zhang Puliang
Published: February 2023

With the rapid development of mobile communication technology, work connectivity behaviors are becoming increasingly pervasive, which has gradually attracted extensive attention from scholars and practitioners. According to the work-home resource model, we propose a theoretical model that proactive/passive work connectivity behaviors induce family harmony through self-efficacy and ego depletion, and we explore the moderating role of family support in this relationship. Based on survey data collected from 364 questionnaires using a three-wave time-lagged design, the results show that: (1) Proactive work connection behaviors have a negative effect on family harmony; Passive work connection behaviors have a negative effect on family harmony. (2) Self-efficacy plays a suppressing role in the relationship between proactive work connection behaviors and family harmony. (3) Ego depletion plays a mediating role in the relationship between passive work connectivity behaviors and family harmony; (4) Family support not only positively moderates the relationship between proactive work connectivity behaviors and self-efficacy, but also moderates the suppressing effect of proactive work connectivity behaviors on family harmony through self-efficacy; (5) Family support not only negatively moderates the relationship between passive work connectivity behaviors and ego depletion, but also moderates the mediating effect of passive work connectivity behaviors on family harmony through ego depletion. The above results can broaden our understanding of the effect of work connectivity behaviors and provide some inspiration for how to optimize the management strategy of employees' work connectivity behaviors.
RETRACTED: Therapeutic interventions impact brain function and promote post-traumatic growth in adults living with post-traumatic stress disorder: A systematic review and meta-analysis of functional magnetic resonance imaging studies
Authors: Pierce Zachary P. , Johnson Emily R. , Kim Isabelle A. , Lear Brianna E. , Mast A. Michaela , Black Jessica M.
Published: February 2023

Introduction

The present systematic review and meta-analysis explores the impacts of cognitive processing therapy (CPT), eye movement desensitization and reprocessing (EMDR), and prolonged exposure (PE) therapy on neural activity underlying the phenomenon of post-traumatic growth for adult trauma survivors.

Methods

We utilized the following databases to conduct our systematic search: Boston College Libraries, PubMed, MEDLINE, and PsycINFO. Our initial search yielded 834 studies for initial screening. We implemented seven eligibility criteria to vet articles for full-text review. Twenty-nine studies remained for full-text review after our systematic review process was completed. Studies were subjected to several levels of analysis. First, pre-and post- test post-traumatic growth inventory (PTGI) scores were collected from all studies and analyzed through a forest plot using Hedges’ g. Next, Montreal Neurological Institute (MNI) coordinates and t-scores were collected and analyzed using an Activation Likelihood Estimation (ALE) to measure brain function. T-scores and Hedges’ g values were then analyzed using Pearson correlations to determine if there were any relationships between brain function and post-traumatic growth for each modality. Lastly, all studies were subjected to a bubble plot and Egger’s test to assess risk of publication bias across the review sample.

Results

Forest plot results indicated that all three interventions had a robust effect on PTGI scores. ALE meta-analysis results indicated that EMDR exhibited the largest effect on brain function, with the R thalamus (t = 4.23, p < 0.001) showing robust activation, followed closely by the R precuneus (t = 4.19, p < 0.001). Pearson correlation results showed that EMDR demonstrated the strongest correlation between increased brain function and PTGI scores (r = 0.910, p < 0.001). Qualitative review of the bubble plot indicated no obvious traces of publication bias, which was corroborated by the results of the Egger’s test (p = 0.127).

Discussion

Our systematic review and meta-analysis showed that CPT, EMDR, and PE each exhibited a robust effect on PTG impacts across the course of treatment. However, when looking closer at comparative analyses of neural activity (ALE) and PTGI scores (Pearson correlation), EMDR exhibited a more robust effect on PTG impacts and brain function than CPT and PE.
Psychometric properties of the mental health continuum-short form in Iranian adolescents
Authors: Yousefi Afrashteh Majid , Janjani Parisa
Published: February 2023

Introduction

Psychological tests are necessary to assess the mental state of individuals. Mental health is one of the important psychological indicators and is increasingly considered as having various aspects of well-being. The Mental Health Continuum-Short Form (MHC-SF) is a 14-item instrument that assesses mental health, focusing on emotional, psychological, and social well-being. The present study, the psychometric properties of the Persian version of the MHC-SF were examined in relation to its factor structure, internal consistency, construct validity, and gender measurement invariance among adolescents.

Methods

The population of this study was Iranian adolescents between 11-and 18-year-old who were enrolled in the seventh to twelfth grades. A convenience sample of 822 Adolescents from four large cities in the Iran (Tehran, Zanjan, Hamedan and Ghazvin) participated in the present study. Questionnaires were completed online. Statistical analyses to evaluate the factor structure, internal consistency, construct validity, gender and age factorial invariance were performed in SPSS and LISREL.

Results

According to the results of confirmatory factor analysis, the MHC-SF is composed of three factors: emotional, psychological, and social well-being. Reliability was confirmed by Cronbach’s alpha method and composite reliability (>0.7). Measurement invariance were confirmed among girls and boys. Convergent and divergent validity were also evaluated and confirmed by correlating the test score with similar and different tests.

Conclusion

This study confirmed the psychometric properties of MHC-SF in the Iranian adolescent community. This instrument can be used in psychological research and diagnostic evaluations.
The double-edged sword effects of active social media use on loneliness: The roles of interpersonal satisfaction and fear of missing out
Authors: Mao Jian , Fu Ge-xi , Huang Jian-jun
Published: February 2023

Introduction

Social media' impact on loneliness has attracted widespread scholarly attention. One hypothesis is that active social media use (ASMU) is associated with a decrease in loneliness. However, several empirical studies did not find a significant correlation between ASMU and loneliness, and ASMU may even increase loneliness. This study explored the mechanism of the double-edged sword effects of ASMU on loneliness.

Methods

Data were collected through convenience sampling from three universities in China. A total of 454 Chinese college social media users (Mean age 19.75 ± 1.33; 59.92% female) completed an online questionnaire.

Results

ASMU was positively related to interpersonal relationship satisfaction, which was negatively related to general trait-fear of missing out (FoMO) and loneliness. Further structural equation modeling (SEM) analysis showed that ASMU could negatively predict loneliness through the mediation pathways of interpersonal satisfaction and “Interpersonal satisfaction → Trait-FoMO.” At the same time, ASMU was also positively associated with online-specific state-FoMO, which was positively associated with trait-FoMO and loneliness. Further SEM analysis found no mediation effect of state-FoMO between ASMU and loneliness, but state-FoMO and traitFoMO sequentially mediate the relationship between ASMU and loneliness.

Discussion

This study indicates that ASMU may increase and decrease loneliness. Interpersonal satisfaction and FoMO explained the double-edged mechanism of ASMU on loneliness. These findings contribute to dialectically understanding the effectiveness of active social media use and provide theoretical guidance for promoting the beneficial aspects of social media while weakening its harmful consequences.
Don’t worry about the anchor-item setting in longitudinal learning diagnostic assessments
Authors: Yu Xinyue , Zhan Peida , Chen Qipeng
Published: February 2023

Previous longitudinal assessment experiences for multidimensional continuous latent constructs suggested that the set of anchor items should be proportionally representative of the total test forms in content and statistical characteristics and that they should be loaded on every domain in multidimensional tests. In such cases, the set of items containing the unit Q-matrix, which is the smallest unit representing the whole test, seems to be the natural choice for anchor items. Two simulation studies were conducted to verify the applicability of these existing insights to longitudinal learning diagnostic assessments (LDAs). The results mainly indicated that there is no effect on the classification accuracy regardless of the unit Q-matrix in the anchor items, and even not including the anchor items has no impact on the classification accuracy. The findings of this brief study may ease practitioners’ worries regarding anchor-item settings in the practice application of longitudinal LDAs.