bluuebunny/crossref_metadata_2025 huggingface dataset:
Economic Implications of Chronic Sinusitis
Authors: Gliklich Richard E. , Metson Ralph
Published: September 2022

An approach to cost analysis useful in understanding the economic implications of surgical intervention on chronic sinusitis is break‐even time analysis. The break‐even time is the time until cost savings associated with improved health status after surgery equal the upfront costs of the operation itself. Data from 100 consecutive patients undergoing sinus operation were obtained by survey before surgery and at quarterly intervals for 1 year with statistically validated outcome measures (Medical Outcome Study Short Form 36‐Item Health Survey, Chronic Sinusitis Survey). Direct and indirect costs were obtained or derived for this cohort. The cost of sinus medications, including over‐the‐counter remedies, nasal steroid sprays, and antibiotics, averaged $1220 per patient per year before surgery and $629 after surgery (p < 0.0001), which is a 48% reduction. Surgical costs averaged $6490 per patient. Economic modeling predicted a break‐even time of approximately 7 years assuming a 3% surgical revision rate per year, a 3% decrease in sickness‐related disability, and a 5% discount rate. The model was sensitive to changes in the total cost of operation, the surgical revision rate, and the anticipated disability benefit. We conclude that significant direct and indirect medical cost savings may be achieved after surgical intervention for chronic sinusitis and these savings eventually break even with the total cost of surgery itself.
Sudden Deafness: Lack of Evidence for Systemic Viral Infection
Authors: Pitkäranta Anne , Julkunen Ilkka
Published: September 2022

The cause of sudden deafness remains unknown even though available evidence suggests that viral infection could be one factor involved. The presence of an interferon‐inducible protein termed MxA in patients' leukocytes is a good marker of a systemic viral infection. This study included 20 patients with sudden deafness and 12 control subjects. Peripheral blood leukocytes obtained from the patients with sudden deafness did not express significantly higher levels of interferon‐α/β‐inducible MxA protein than control subjects. In addition, no measurable interferon‐α activity was detected in any of the serum specimens. These findings suggest that sudden deafness is not commonly associated with a systemic viral infection.
Microdata collection and openness in the Middle East and North Africa
Authors: Ekhator-Mobayode Uche E. , Hoogeveen Johannes
Published: September 2022

This article uses a “mystery client” approach and visits the websites of National Statistical Offices and international microdata libraries to assess whether foundational microdata sets for countries in the Middle East and North Africa region are collected, up to date, and made available to researchers. The focus is on population and economic censuses, price data and consumption, labor, health, and establishment surveys. The results show that about half of the expected core data sets are being collected and that only a fraction is made available publicly. As a consequence, many summary statistics, including national accounts and welfare estimates, are outdated and of limited relevance to decision-makers. Additional investments in microdata collection and publication of the data once collected are strongly advised.
THE HAIHUNHOU CAPSULE BIOGRAPHIES OF KONGZI AND HIS DISCIPLES
Authors: Csikszentmihalyi Mark
Published: September 2022

This article introduces the biographical texts accompanying illustrations of Kongzi and several disciples on the wooden frame and cover of a mirror stand excavated in 2015 from the Haihunhou tomb near Nanchang. These texts are analyzed with reference to evolving portrayals of these figures in the Western Han, paying particular attention to parallels with two generically similar chapters in theShi ji(Records of the Archivist). Of particular interest is the way the excavated disciple biographies share biographical elements with transmitted counterparts, but select different dialogues for each disciple, most of which are also found in theLun yu(Analects). This suggests that the artists who created the mirror stand relied on a different source text from the compilers of theShi jichapter, perhaps on a pairing of visual and biographical information about the disciples calledKongzi dizi(Kongzi's disciples). The biographies also evince a heightened emphasis on the disciples and Kongzi's judgments about them, consistent with the Han view that the proper selection of ministers was a key aspect of the master's “Kingly Way.”
Congenital aural atresia: hearing rehabilitation using active middle-ear implants
Authors: Thurnheer S , Müller T , Linder T , Simmen D , Harder M
Published: September 2022

Objective

To assess hearing rehabilitation in patients with congenital aural atresia using an active middle-ear implant (Vibrant Soundbridge).

Methods

Of a cohort of 70 microtia and atresia patients, 10 underwent Vibrant Soundbridge implantation between 2008 and 2021. Two of the 10 patients had binaural implantation, resulting in 12 ears for analysis. Pre- and post-operative audiometry data were analysed, and patient satisfaction was evaluated. Surgical issues regarding coupling sites and outcomes were analysed.

Results

Pure tone average (0.5, 1, 2 and 4 kHz) improved from a pre-operative mean (standard deviation) of 65.3 (8.7) dB HL to a post-operative mean of 26.8 (4.9) dB HL. This resulted in a mean pure tone average gain of 38.5 dB HL. The results indicate no obvious difference between stapes (n = 8) and incus (n = 4) coupling. The mean effective gain for 0.5, 1, 2 and 4 kHz was −17.8 dB HL (standard deviation = 4.3). Concerning effective gain, Vibrant Soundbridge performed best at 2 kHz. Patients reported high overall satisfaction, good sound quality and strongly improved directional hearing.

Conclusion

An active middle-ear implant (Vibrant Soundbridge) allows hearing rehabilitation in selected atretic ears, and provides long-term hearing stability in children and adults.
Governance and human rights implications of ASEAN's Smart Cities Network: a knowledge commons analysis
Authors: de Jonge Alice
Published: September 2022

Launched in April 2018, the Association of Southeast Asian Nations' Smart Cities Network (ASCN) initiative raises important issues regarding the tensions between achieving smart city objectives on the one hand and protection of human rights on the other. The aim of this paper is to explore these tensions using a Knowledge Commons Framework analysis. I first analyse the three key pillars of the ASCN pilot city knowledge commons – knowledge resources, community attributes and governance ‘rules in use’ – using human rights criteria. I the apply the lessons of this analysis to two fundamental aspects of human experience in smart city contexts – mobility through transport systems and access to essential services through energy supply.
International crimes through the lens of global constitutionalism
Authors: Zysset Alain
Published: September 2022

Scholars of global constitutionalism have recently come to examine international criminal law (ICL) and its associated institutions, in particular the International Criminal Court (the ICC). This article prolongs these efforts by pointing to and remedying two deficits of that project with particular emphasis on the Rome Statute crimes. First, how does one account for the role of the international trial in global constitutionalist terms? Second, can global constitutionalism insightfully explain the content and scope of these crimes – that is, both their substantive definition and the predominant modes of liability developed by the ICC? This article answers both questions affirmatively and offers an account of their nexus. It first shows that the Rome Statute crimes are often perpetrated through a hierarchically organized apparatus of control, and interprets their global constitutional significance via the principle of constituent power. It then makes use of Antony Duff’s relational account of criminal liability to offer an account of the international trial. In the international context, one can conceive of the trial as allowing state or state-like authorities to call each other to account, which renders justice to the core function of enabling and limiting political authority on which global constitutionalism centres.
The Combined Impact of Canopy Stability and Soil NOx Exchange on Ozone Removal in a Temperate Deciduous Forest
Authors: Visser Auke J. , Ganzeveld Laurens N. , Finco Angelo , Krol Maarten C. , Marzuoli Riccardo , Boersma K. Folkert
Published: September 2022

Dry deposition is an important ozone sink that impacts ecosystem carbon and water cycling. Ozone dry deposition in forests is regulated by vertical transport, stomatal uptake, and non‐stomatal processes including chemical removal. However, accurate descriptions of these processes in deposition parameterizations are hindered by sparse observational constraints on individual sink terms. Here we quantify the contribution of canopy‐atmosphere turbulent exchange and chemical ozone removal by soil‐emitted nitric oxide (NO) to ozone deposition in a North‐Italian broadleaf deciduous forest. We apply a multi‐layer canopy exchange model to interpret campaign observations of nitrogen oxides (NOx = NO + NO2) and ozone exchange above and inside the forest canopy. Two state‐of‐science parameterizations of in‐canopy vertical diffusivity, based on above‐canopy wind speed or stability, do not reproduce the observed exchange suppressed by canopy‐top radiative heating, resulting in overestimated dry deposition velocities of 10%–19% during daytime. Applying observation‐derived vertical diffusivities in our simulations largely resolves this overestimation. Soil emissions are an important NOx source despite the observed high background NOx levels. Soil NOx emissions decrease the gradient between canopy and surface layer NOx mixing ratios, which suppresses simulated NOx deposition by 80% compared to a sensitivity simulation without soil emissions. However, a sensitivity analysis shows that the enhanced chemical ozone sink by reaction with soil‐emitted NO is offset by increased vertical ozone transport from aloft and suppressed dry deposition. Our results highlight the need for targeted observations of non‐stomatal ozone removal and turbulence‐resolving deposition simulations to improve quantification and model representation of forest ozone deposition.
Apportionment of the Pre‐Industrial to Present‐Day Climate Forcing by Methane Using UKESM1: The Role of the Cloud Radiative Effect
Authors: O’Connor Fiona M. , Johnson Ben T. , Jamil Omar , Andrews Timothy , Mulcahy Jane P. , Manners James
Published: September 2022

The Year 1850 to 2014 increase in methane from 808 to 1831 ppb leads to an effective radiative forcing (ERF) of 0.97 ± 0.04 W m−2 in the United Kingdom's Earth System Model, UKESM1. The direct methane contribution is 0.54 ± 0.04 W m−2. It is better represented in UKESM1 than in its predecessor model HadGEM2 due to shortwave and longwave absorption improvements and the absence of an anomalous dust response in the UKESM1 simulations. An indirect ozone ERF of 0.13–0.20 W m−2 is due to the tropospheric ozone increase outweighing that of the stratospheric decrease. The indirect water vapor ERF of 0.02–0.07 W m−2 is consistent with previous estimates. The methane increase also leads to a cloud radiative effect of 0.12 ± 0.02 W m−2 from thermodynamic adjustments and aerosol‐cloud interactions (aci). Shortwave and longwave contributions of 0.23 and −0.35 W m−2 to the cloud forcing arise from radiative heating and stabilization of the upper troposphere, reducing convection and global cloud cover. The aerosol‐mediated contribution (0.28–0.30 W m−2) is due to changes in oxidants reducing new particle formation (−8%), shifting the aerosol size distribution toward fewer but larger particles. Cloud droplet number concentration decreases and cloud droplet effective radius increases. This reduction in the Twomey effect switches the cloud forcing sign (−0.14 to 0.12 W m−2) and is due to chemistry‐aerosol‐cloud coupling in UKESM1. Despite uncertainties in rapid adjustments and process representation in models, these results highlight the potential importance of chemistry‐aerosol‐cloud interactions and dynamical adjustments in climate forcing.
The International Reference Ionosphere Model: A Review and Description of an Ionospheric Benchmark
Authors: Bilitza Dieter , Pezzopane Michael , Truhlik Vladimir , Altadill David , Reinisch Bodo W. , Pignalberi Alessio
Published: September 2022

This paper is a review of the International Reference Ionosphere (IRI) project and model. IRI is recognized as the official standard for the Earth's ionosphere by the International Standardization Organization, the International Union of Radio Science, the Committee on Space Research, and the European Cooperation for Space Standardization. As requested by these organizations, IRI is an empirical (data‐based) model representing the primary ionospheric parameters based on the long data record that exists from ground and space observations of the ionosphere. The core model describes monthly averages of the electron density, electron temperature, ion temperature, and ion composition globally in the altitude range from 60 to 2,000 km. Over time additional parameters were added in response to requests from the user community, this includes the equatorial ion drift, the occurrence probability of spread‐F and of an F1 layer, auroral boundaries and the electron content from the bottom of the ionosphere to user‐specified altitude. IRI has undergone extensive validations and is used for a wide range of applications in science, engineering, and education. This review is the result of many requests we have received for a comprehensive description of the model. It is also meant as a guide for users who are interested in a deeper understanding of the model architecture and its mathematical formalism.
Integrated optical-readout of a high-Q mechanical out-of-plane mode
Authors: Guo Jingkun , Gröblacher Simon
Published: September 2022

The rapid development of high-QM macroscopic mechanical resonators has enabled great advances in optomechanics. Further improvements could allow for quantum-limited or quantum-enhanced applications at ambient temperature. Some of the remaining challenges include the integration of high-QM structures on a chip, while simultaneously achieving large coupling strengths through an optical read-out. Here, we present a versatile fabrication method, which allows us to build fully integrated optomechanical structures. We place a photonic crystal cavity directly above a mechanical resonator with high-QM fundamental out-of-plane mode, separated by a small gap. The highly confined optical field has a large overlap with the mechanical mode, enabling strong optomechanical interaction strengths. Furthermore, we implement a novel photonic crystal design, which allows for a very large cavity photon number, a highly important feature for optomechanical experiments and sensor applications. Our versatile approach is not limited to our particular design but allows for integrating an out-of-plane optical read-out into almost any device layout. Additionally, it can be scaled to large arrays and paves the way to realizing quantum experiments and applications with mechanical resonators based on high-QM out-of-plane modes alike.
A critical evaluation of dynamical systems models of bipolar disorder
Authors: Nunes Abraham , Singh Selena , Allman Jared , Becker Suzanna , Ortiz Abigail , Trappenberg Thomas , Alda Martin
Published: September 2022

Bipolar disorder (BD) is a mood disorder involving recurring (hypo)manic and depressive episodes. The inherently temporal nature of BD has inspired its conceptualization using dynamical systems theory, which is a mathematical framework for understanding systems that evolve over time. In this paper, we provide a critical review of the dynamical systems models of BD. Owing to the heterogeneity of methodological and experimental designs in computational modeling, we designed a structured approach that parallels the appraisal of animal models by their face, predictive, and construct validity. This tool, the validity appraisal guide for computational models (VAG-CM), is not an absolute measure of validity, but rather a guide for a more objective appraisal of models in this review. We identified 26 studies published before November 18, 2021 that proposed generative dynamical systems models of time-varying signals in BD. Two raters independently applied the VAG-CM to the included studies, obtaining a mean Cohen’s κ of 0.55 (95% CI [0.45, 0.64]) prior to establishing consensus ratings. Consensus VAG-CM ratings revealed three model/study clusters: data-driven models with face validity, theory-driven models with predictive validity, and theory-driven models lacking all forms of validity. We conclude that future modeling studies should employ a hybrid approach that first operationalizes BD features of interest using empirical data to achieve face validity, followed by explanations of those features using generative models with components that are homologous to physiological or psychological systems involved in BD, to achieve construct validity. Such models would be best developed alongside long-term prospective cohort studies involving a collection of multimodal time-series data. We also encourage future studies to extend, modify, and evaluate the VAG-CM approach for a wider breadth of computational modeling studies and psychiatric disorders.
The alternative splicing of intersectin 1 regulated by PTBP1 promotes human glioma progression
Authors: Lan Chungen , Zhang Huikun , Wang Kezhen , Liu Xiaoli , Zhao Yawen , Guo Zhifang , Zhang Ning , Zhou Yongxia , Gao Manzhi , Gu Feng , Ma Yongjie
Published: September 2022

Intersectin 1 (ITSN1) contains two isoforms: ITSN1-S and ITSN1-L, which are highly regulated by alternative splicing. Our previous results showed that the two isoforms of ITSN1 displayed opposite functions: ITSN1-S promoted glioma development, while ITSN1-L exerted an inhibitory role in glioma progression. In this study, our transcriptome analysis using a large glioma cohort indicated that the ratio of ITSN1-S/ITSN1-L was positively correlated with glioma grading and poor prognosis. We identified the RNA-binding protein polypyrimidine tract-binding protein 1 (PTBP1) as an ITSN1 pre-mRNA interaction protein through RNA pull-down assay and RNA immunoprecipitation assay. Knockdown of PTBP1 decreased the ratio of ITSN1-S/ITSN1-L. Minigene reporter assay and mutation analyses further confirmed PTBP1 targeted polypyrimidine sequences on ITSN1 exon 30 (TTGCACTTCAGTATTTT) and promoted the inclusion of ITSN1 exon 30. Subsequently, silencing PTBP1 inhibited glioma cell proliferation, migration, and invasion by down-regulating the ratio of ITSN1-S/ITSN1-L. Taken together, our study provides a novel mechanism that PTBP1 modulates the alternative splicing of ITSN1 and promotes glioma proliferation and motility by up-regulating the ratio of ITSN1-S/ITSN1-L, thereby highlighting that PTBP1 may be an attractive therapeutic target for gliomas.
Two valid and reliable tests for monitoring age-related memory performance and neophobia differences in dogs
Authors: Piotti Patrizia , Piseddu Andrea , Aguzzoli Enrica , Sommese Andrea , Kubinyi Eniko
Published: September 2022

The prolonged lifespan of companion dogs has resulted in increased behavioural and physical challenges linked to old age. The development of behavioural tests to identify and monitor age-related differences has begun. However, standardised testing requires validation. The present study aimed to assess external validity, interobserver reliability, and test–retest reliability of an indoor test battery for the rapid assessment of age-related behavioural differences in dogs. Two experimenters tested young dogs (N = 20, mean age ± SD = 2.7 ± 0.4 years) and old dogs (N = 18, mean age ± SD = 11.8 ± 1.3 years) in the test battery once and then again after two weeks. Our results found external validity for two subtests out of six. On both test occasions, old dogs committed more errors than young dogs in a memory subtest and showed more object avoidance when encountering a novel object. Interobserver reliability and test–retest reliability was high. We conclude that the Memory and Novel object subtests are valid and reliable for monitoring age-related memory performance and object neophobic differences in dogs.
Paradoxically lowered oxygen isotopes of hydrothermally altered minerals by an evolved magmatic water
Authors: Wei Chun-Sheng , Zhao Zi-Fu
Published: September 2022

It has been well known that the influxing meteoric water can hydrothermally lower oxygen and hydrogen isotopes of rocks and/or minerals during continental magmatic or metamorphic processes in certain appropriate cases. Its opposite, however, is not implicitly true and needs independent testing. In terms of a novel procedure recently proposed for dealing with thermodynamic re-equilibration of oxygen isotopes between constituent minerals and water from fossil hydrothermal systems, the initial oxygen isotopes of water ($${\updelta }^{18}{\text{O}}_{\text{W}}^{\text{i}}$$

δ

18

O

W

i

) are theoretically inverted from the early Cretaceous post-collisional granitoids and Triassic gneissic country rock across the Dabie orogen in central-eastern China. Despite ancient meteoric waters with low $${\updelta }^{18}{\text{O}}_{\text{W}}^{\text{i}}$$

δ

18

O

W

i

value down to − 11.01 ± 0.43‰ (one standard deviation, 1SD), oxygen isotopes of hydrothermally altered rock-forming minerals from a granitoid were unexpectedly but concurrently lowered by an evolved magmatic water with mildly high $${\updelta }^{18}{\text{O}}_{\text{W}}^{\text{i}}$$

δ

18

O

W

i

value of 2.81 ± 0.05‰ at 375 °C with a water/rock (W/R)c ratio of 1.78 ± 0.20 for the closed system. The lifetime of fossil hydrothermal systems studied herein is kinetically constrained to no more than 1.2 million years (Myr) via surface-reaction oxygen exchange in the late-stage of continental magmatism or metamorphism. Thereby, caution should be paid when lowered oxygen isotopes of hydrothermally altered rocks and/or minerals were intuitively and/or empirically inferred from the external infiltration of the purely meteoric water with a low $${\updelta }^{18}{\text{O}}_{\text{W}}^{\text{i}}$$

δ

18

O

W

i

value alone.
Research on the matching characteristics of the theoretical digging force of a backhoe hydraulic excavator
Authors: Ren Zhigui , Li Jiahao , Pang Xiaoping , Liu Jurong , Li Tianyu , Yu Songsong
Published: September 2022

The theoretical digging force is the maximum digging resistance that an excavator can overcome, which is an important measure of its digging capacity. To study the matching of the digging capacity with the actual demand and the matching of the working device mechanism, a 36.5 t backhoe hydraulic excavator is used as an example to analyse the distributions of the digging resistance under two different normal digging area working conditions and the maximum digging resistance characteristics of the tool. An appropriate digging postures are selected, based on limit digging force and compound digging force models, the theoretical digging forces under the two working conditions are obtained and matched with the measured digging resistance force values and the limiting factors affecting the digging force. The results show that the average percentage of theoretical digging forces greater than the measured digging resistance under both calculation models is 84.06% rather than 100%. The results of different digging methods all indicate that small chamber locking of the boom cylinder is too often the limiting factor for the digging force, resulting in poor matching of the working device. This study provides guidance for the improvement of the theoretical digging force model and the evaluation of the matching characteristics of the working device.
A novel method for estimating subresolution porosity from CT images and its application to homogeneity evaluation of porous media
Authors: Zhuang Li , Shin Hyu-Soung , Yeom Sun , Pham Chuyen Ngoc , Kim Young-Jae
Published: September 2022

We propose a new method, i.e., the statistical phase fraction (SPF) method, to estimate the total porosity and spatial distribution of local porosities from subresolution pore-dominated X-ray microtomography images of porous materials. The SPF method assumes that a voxel in a CT image is composed of either a single or a maximum of three pure phases of matter (solid, liquid and air). Gaussian function (GF) fitting is conducted on the basis that the summation of the area of each GF curve is equal to the total area covered by the CT histogram. The volume fraction of each phase corresponding to each GF is calculated based on the mean value of the GF, the area of the GF, and the CT numbers for pure phases. The SPF method is verified on three different types of components containing only air and solid phases, i.e., alumina ceramic and two sintered lunar regolith simulants with relatively homogenous and inhomogeneous microstructures. The estimated porosities of a total of 15 specimens (the total porosity ranges from 0 to 51%) via the SPF method show an average error of 3.11% compared with the ground truth. Spatial distribution of local porosities in the defined representative element volume is investigated for homogeneity evaluation. Results show that the local porosity inhomogeneity in the sintered FJS-1 specimens is more prominent than that in the sintered KLS-1 specimens.
The evolutionary footprint of influenza A subtype H3N2 strains in Bangladesh: implication of vaccine strain selection
Authors: Rahman Sezanur , Hasan Mehedi , Alam Md Shaheen , Uddin K. M. Main , Moni Sayra , Rahman Mustafizur
Published: September 2022

In February each year, World Health Organization (WHO) recommends candidate vaccine viruses for the forthcoming northern hemisphere (NH) season; however, the influenza season in the temperate zone of NH begins in October. During egg- or cell culture-propagation, the vaccine viruses become too old to confer the highest match with the latest strains, impacting vaccine effectiveness. Therefore, an alternative strategy like mRNA-based vaccine using the most recent strains should be considered. We analyzed influenza A subtype H3N2 strains circulating in NH during the last 10 years (2009–2020). Phylogenetic analysis revealed multiple clades of influenza strains circulating every season, which had substantial mismatches with WHO-recommended vaccine strains. The clustering pattern suggests that influenza A subtype H3N2 strains are not fixed to the specific geographical region but circulate globally in the same season. By analyzing 39 seasons from eight NH countries with the highest vaccine coverage, we also provide evidence that the influenza A, subtype H3N2 strains from South and Southeast Asia, including Bangladesh, had the highest genetic proximity to the NH strains. Furthermore, insilico analysis showed minimal effect on the Bangladeshi HA protein structure, indicating the stability of Bangladeshi strains. Therefore, we propose that Bangladeshi influenza strains represent genetic makeup that may better fit and serve as the most suitable candidate vaccine viruses for the forthcoming NH season.
Differential transcriptomic responses to heat stress in surface and subterranean diving beetles
Authors: Beasley-Hall Perry G. , Bertozzi Terry , Bradford Tessa M. , Foster Charles S. P. , Jones Karl , Tierney Simon M. , Humphreys William F. , Austin Andrew D. , Cooper Steven J. B.
Published: September 2022

Subterranean habitats are generally very stable environments, and as such evolutionary transitions of organisms from surface to subterranean lifestyles may cause considerable shifts in physiology, particularly with respect to thermal tolerance. In this study we compared responses to heat shock at the molecular level in a geographically widespread, surface-dwelling water beetle to a congeneric subterranean species restricted to a single aquifer (Dytiscidae: Hydroporinae). The obligate subterranean beetle Paroster macrosturtensis is known to have a lower thermal tolerance compared to surface lineages (CTmax 38 °C cf. 42–46 °C), but the genetic basis of this physiological difference has not been characterized. We experimentally manipulated the thermal environment of 24 individuals to demonstrate that both species can mount a heat shock response at high temperatures (35 °C), as determined by comparative transcriptomics. However, genes involved in these responses differ between species and a far greater number were differentially expressed in the surface taxon, suggesting it can mount a more robust heat shock response; these data may underpin its higher thermal tolerance compared to subterranean relatives. In contrast, the subterranean species examined not only differentially expressed fewer genes in response to increasing temperatures, but also in the presence of the experimental setup employed here alone. Our results suggest P. macrosturtensis may be comparatively poorly equipped to respond to both thermally induced stress and environmental disturbances more broadly. The molecular findings presented here have conservation implications for P. macrosturtensis and contribute to a growing narrative concerning weakened thermal tolerances in obligate subterranean organisms at the molecular level.
Experimental evidence on improving COVID-19 vaccine outreach among migrant communities on social media
Authors: Tjaden Jasper , Haarmann Esther , Savaskan Nicolai
Published: September 2022

Studies from several countries suggest that COVID-19 vaccination rates are lower among migrants compared to the general population. Urgent calls have been made to improve vaccine outreach to migrants, however, there is limited evidence on effective approaches, especially using social media. We assessed a targeted, low-cost, Facebook campaign disseminating COVID-19 vaccine information among Arabic, Turkish and Russian speakers in Germany (N = 888,994). As part of the campaign, we conducted two randomized, online experiments to assess the impact of the advertisement (1) language and (2) depicted messenger (government authority, religious leader, doctor or family). Key outcomes included reach, click-through rates, conversion rates and cost-effectiveness. Within 29 days, the campaign reached 890 thousand Facebook users. On average, 2.3 individuals accessed the advertised COVID-19 vaccination appointment tool for every euro spent on the campaign. Migrants were 2.4 (Arabic), 1.8 (Russian) and 1.2 (Turkish) times more likely to click on advertisements translated to their native language compared to German-language advertisements. Furthermore, findings showed that government representatives can be more successful in engaging migrants online compared to other messengers, despite common claims of lower trust in government institutions among migrants. This study highlights the potential of tailored, and translated, vaccination campaigns on social media for reaching migrants who may be left out by traditional media campaigns.
Exploring the ability of stroke survivors in using the contralesional hemisphere to control a brain–computer interface
Authors: Mansour Salem , Giles Joshua , Ang Kai Keng , Nair Krishnan P. S. , Phua Kok Soon , Arvaneh Mahnaz
Published: September 2022

Brain-computer interfaces (BCIs) have recently been shown to be clinically effective as a novel method of stroke rehabilitation. In many BCI-based studies, the activation of the ipsilesional hemisphere was considered a key factor required for motor recovery after stroke. However, emerging evidence suggests that the contralesional hemisphere also plays a role in motor function rehabilitation. The objective of this study is to investigate the effectiveness of the BCI in detecting motor imagery of the affected hand from contralesional hemisphere. We analyzed a large EEG dataset from 136 stroke patients who performed motor imagery of their stroke-impaired hand. BCI features were extracted from channels covering either the ipsilesional, contralesional or bilateral hemisphere, and the offline BCI accuracy was computed using 10$$\times $$×10-fold cross-validations. Our results showed that most stroke patients can operate the BCI using either their contralesional or ipsilesional hemisphere. Those with the ipsilesional BCI accuracy of less than 60% had significantly higher motor impairments than those with the ipsilesional BCI accuracy above 80%. Interestingly, those with the ipsilesional BCI accuracy of less than 60% achieved a significantly higher contralesional BCI accuracy, whereas those with the ipsilesional BCI accuracy more than 80% had significantly poorer contralesional BCI accuracy. This study suggests that contralesional BCI may be a useful approach for those with a high motor impairment who cannot accurately generate signals from ipsilesional hemisphere to effectively operate BCI.
[68Ga]FAPI-PET/CT for radiation therapy planning in biliary tract, pancreatic ductal adeno-, and adenoidcystic carcinomas
Authors: Guberina Nika , Kessler Lukas , Pöttgen Christoph , Guberina Maja , Metzenmacher Martin , Herrmann Ken , Mucha Maja , Rischpler Christoph , Indenkämpen Frank , Siveke Jens T. , Treckmann Jürgen , Umutlu Lale , Kasper Stefan , Fendler Wolfgang P. , Stuschke Martin
Published: September 2022

Biliary-tract-carcinomas (BTC), pancreatic-ductal-adenocarcinomas (PDAC) and adenoidcystic-carcinomas (AC) have in common that they are traditionally treated with large clinical-target-volumes (CTV). The aim of this study is to examine the impact of pretreatment-[68Ga]FAPI-PET/CT on target-volume-definition and posttreatment-[68Ga]FAPI-PET/CT-response-assessment for BTC-, PDAC- and AC-patients referred to radiation-therapy. All consecutive BTC-, PDAC-, and AC-patients who received pretreatment-[68Ga]FAPI-PET/CT±[18F]FDG-PET/CT were included from 01.01.2020 to 01.03.2022. MTV and SUVmax were separately generated based on [68Ga]FAPI- and [18F]FDG-PET/CT-images. A [68Ga]FAPI- and [18F]FDG-based-CTV was defined. Treatment-plans were compared. Treatment-response was reassessed by a second [68Ga]FAPI-PET/CT and [18F]FDG-PET/CT after treatment-completion. Intermodality comparison of lesion-to-background-ratios [SUVmax_lesion/SUVmean_background] for individual timepoints t1 and t2 revealed significant higher values for [68Ga]FAPI compared to [18F]FDG (t1, p = 0.008; t2, p = 0.005). Intermodality comparison of radiation-therapy-plans showed that [68Ga]FAPI-based planning resulted in D100% = 97.2% and V95% = 98.8% for the [18F]FDG-MTV. [18F]FDG-based-planning resulted in D100% = 35.9% and V95% = 78.1% for [68Ga]FAPI-MTV. [18F]FDG-based-planning resulted only in 2 patients in V95% > 95% for [68Ga]FAPI-MTV, and in 1 patient in D100% > 97% for [68Ga]FAPI-MTV. GTV-coverage in terms of V95% was 76.4% by [18F]FDG-based-planning and 99.5% by [68Ga]FAPI-based-planning. Pretreatment [68Ga]FAPI-PET/CT enhances radiation-treatment-planning in this particular group of patients. While perilesional and tumoral follow-up [18F]FDG-uptake behaved uniformly, perilesional and tumoral reaction may differ in follow-up [68Ga]FAPI-imaging. Complementary [68Ga]FAPI- and [18F]FDG-imaging enhance treatment-response-assessment.
Significance of nanoparticles aggregation on the dynamics of rotating nanofluid subject to gyrotactic microorganisms, and Lorentz force
Authors: Ali Bagh , Siddique Imran , Ali Rifaqat , Awrejcewicze Jan , Jarad Fahd , Khalifa Hamiden Abd El-Wahed
Published: September 2022

The significance of nanoparticle aggregation, Lorentz and Coriolis forces on the dynamics of spinning silver nanofluid flow past a continuously stretched surface is prime significance in modern technology, material sciences, electronics, and heat exchangers. To improve nanoparticles stability, the gyrotactic microorganisms is consider to maintain the stability and avoid possible sedimentation. The goal of this report is to propose a model of nanoparticles aggregation characteristics, which is responsible to effectively state the nanofluid viscosity and thermal conductivity. The implementation of the similarity transforQ1m to a mathematical model relying on normal conservation principles yields a related set of partial differential equations. A well-known computational scheme the FEM is employed to resolve the partial equations implemented in MATLAB. It is seen that when the effect of nanoparticles aggregation is considered, the temperature distribution is enhanced because of aggregation, but the magnitude of velocities is lower. Thus, showing the significance impact of aggregates as well as demonstrating themselves as helpful theoretical tool in future bioengineering and industrial applications.
Involvement of endothelins in neuroprotection of valosin-containing protein modulators against retinal ganglion cell damage
Authors: Kusaka Mami , Hasegawa Tomoko , Ikeda Hanako Ohashi , Inoue Yumi , Iwai Sachiko , Iida Kei , Tsujikawa Akitaka
Published: September 2022

We have previously shown that Kyoto University Substances (KUSs), valosin-containing protein (VCP) modulators, suppress cell death in retinal ganglion cells of glaucoma mouse models through alterations of various genes expressions. In this study, among the genes whose expression in retinal ganglion cells was altered by KUS treatment in the N-methyl-d-aspartic acid (NMDA) injury model, we focused on two genes, endothelin-1 (Edn1) and endothelin receptor type B (Ednrb), whose expression was up-regulated by NMDA and down-regulated by KUS treatment. First, we confirmed that the expression of Edn1 and Ednrb was upregulated by NMDA and suppressed by KUS administration in mice retinae. Next, to clarify the influence of KUSs on cell viability in relation to the endothelin signaling, cell viability was examined with or without antagonists or agonists of endothelin and with or without KUS in 661W retinal cells under stress conditions. KUS showed a significant protective effect under glucose-free conditions and tunicamycin-induced stress. This protective effect was partially attenuated in the presence of an endothelin antagonist or agonist under glucose-free conditions. These results suggest that KUSs protect cells partially by suppressing the upregulated endothelin signaling under stress conditions.
Assessment of small in-frame indels and C-terminal nonsense variants of BRCA1 using a validated functional assay
Authors: Nepomuceno Thales C. , dos Santos Ana P. P. , Fernandes Vanessa C. , Elias Anna B. R. , Gomes Thiago T. , Suarez-Kurtz Guilherme , Iversen Edwin S. , Couch Fergus J. , Monteiro Alvaro N. A. , Carvalho Marcelo A.
Published: September 2022

BRCA1 (Breast Cancer 1, early onset) is linked to breast and ovarian cancer predisposition. Still, the risks conferred by a significant portion of BRCA1 variants identified in the population remains unknown. Most of these variants of uncertain significance are missense alterations. However, the functional implications of small in-frame deletions and/or insertions (indels) are also difficult to predict. Our group has previously evaluated the functional impact of 347 missense variants using an extensively validated transcriptional activity assay. Here we show a systematic assessment of 30 naturally occurring in-frame indels located at the C-terminal region of BRCA1. We identified positions sensitive and tolerant to alterations, expanding the knowledge of structural determinants of BRCA1 function. We further designed and assessed the impact of four single codon deletions in the tBRCT linker region and six nonsense variants at the C-terminus end of BRCA1. Amino acid substitutions, deletions or insertions in the disordered region do not significantly impact activity and are not likely to constitute pathogenic alleles. On the other hand, a sizeable fraction of in-frame indels at the BRCT domain significantly impact function. We then use a Bayesian integrative statistical model to derive the probability of pathogenicity for each variant. Our data highlights the importance of assessing the impact of small in-frame indels in BRCA1 to improve risk assessment and clinical decisions for carriers.
Experimental study on uniaxial creep characteristics of sandstone with pre-peak unloading damage
Authors: Liang Zhonghao , Ji Peizhi , Zhang Yifan , Qin Nan , Huang Qiming , Yu Zhuoqun
Published: September 2022

Deep resource extraction has been affected by the complex geological environment of "three highs and one disturbance" for a long time. The surrounding rocks experience strong unloading stress disturbance during the underground resource extraction. The creep characteristics of the perimeter rocks are of great theoretical and practical value. Firstly, the triaxial pre-peak loading and unloading tests on the prepared samples are conducted on the intact rock samples using the TAW-200 rock mechanics test system. Then the rock samples with different degrees of pre-peak unloading damage were prepared under the condition of pre-peak yield. Finally, the uniaxial creep tests were carried out to study the uniaxial creep mechanical properties. The results show that the wave velocities of the damaged rock samples are reduced to different degrees compared with those of the intact rock samples, the creep of the pre-peak unloading damaged rock has a time-dependent damage effect, and the study results are similar to those of the conventional uniaxial creep test of the white sandstone. The instantaneous strain at all stress levels of the damaged rock samples increased gradually with the stress level, which is consistent with the non-linear functional relationship; Based on the time-dependent damage effect, the two-parameter Weibull distribution function was introduced into the West Plains viscoelasticity model, and a creep damage model for white sandstone was proposed. The improved Nishihara model can simulate the uniaxial creep characteristics of each damaged rock sample at various stress levels.
Genetic structure and geneflow of Malus across the Korean Peninsula using genotyping-by-sequencing
Authors: Ha Young-Ho , Gil Hee-Young , Kim Sang-Chul , Choi Kyung , Kim Joo-Hwan
Published: September 2022

This study was to understand the genetic structure and diversity of the KoreanMalusspecies. We used genotyping-by-sequencing (GBS) technology to analyze samples of 112 individuals belonging to 18 populations of wildMalusspp. Using GBS, we identified thousands of single nucleotide polymorphisms in the species analyzed.M. baccataandM. toringo, two dominant mainland species of the Korean Peninsula, were distinguishable based on their genetic structure. However,M. toringocollected from Jeju Island exhibited a different genetic profile than that from the mainland. We identifiedM.cf.micromalusas a hybrid resulting from the Jeju IslandM. toringo(pollen donor) and the mainlandM. baccata, (pollen recipient). PutativeM. mandshuricadistributed on the Korean Peninsula showed a high structural and genetic similarity withM. baccata, indicating that it might be an ecotype. Overall, this study contributes to the understanding of the population history and genetic structure ofMalusin the Korean Peninsula.
Association between carbon monoxide poisoning and adrenal insufficiency: a nationwide cohort study
Authors: Huang Chien-Cheng , Ho Chung-Han , Chen Yi-Chen , Hsu Chien-Chin , Lin Hung-Jung , Wang Jhi-Joung , Su Shih-Bin , Guo How-Ran
Published: September 2022

Carbon monoxide poisoning may damage the brain and adrenal glands, but it is unclear whether it is associated with adrenal insufficiency. We identified all COP patients diagnosed between 1999 and 2012 in Taiwan using the Nationwide Poisoning Database and selected a reference cohort (participants without COP) from the same database by exact matching of age and index date at a 1:2 ratio. Participants with a history of adrenal insufficiency or steroid use of more than 14 days were excluded. We followed up participants until 2013 and compared the risk of developing adrenal insufficiency between the two cohorts. The 21,842 COP patients had a higher risk for adrenal insufficiency than the 43,684 reference participants (adjusted hazard ratio [AHR] = 2.5; 95% confidence interval [CI]: 1.8–3.5) after adjustment for sex and underlying comorbidities (liver disease, thyroid disease, mental disorder). The risk continued to elevate even after 1 year (AHR = 2.1; 95% CI: 1.4–3.4). The COP patients who had acute respiratory failure had an even higher risk for adrenal insufficiency than those without acute respiratory failure, which may indicate a dose–response relationship. Stratified analyses showed that female patients had an elevated risk (AHR = 3.5; 95% CI: 2.1–6.0), but not male patients. Younger patients (< 50 years) had higher risks, and the AHR reached statistical significance in the age groups 20–34 (AHR = 5.5; 95% CI: 1.5–20.6) and 35–49 (AHR = 4.9; 95% CI: 2.3–10.6) years old. The risk for developing adrenal insufficiency elevated after COP, especially in female and younger patients. Carbon monoxide is the most common gaseous agent causing acute intoxication worldwide. Results of the current study call for monitoring adrenal function of patients with COP.
Alzheimer's disease diagnosis by blood plasma molecular fluorescence spectroscopy (EEM)
Authors: dos Santos Ricardo Fernandes , Paraskevaidi Maria , Mann David M. A. , Allsop David , Santos Marfran C. D. , Morais Camilo L. M. , Lima Kássio M. G.
Published: September 2022

Despite tremendous research advances in detecting Alzheimer's disease (AD), traditional diagnostic tests remain expensive, time-consuming or invasive. The search for a low-cost, rapid, and minimally invasive test has marked a new era of research and technological developments toward establishing blood-based AD biomarkers. The current study has employed excitation-emission matrices (EEM) of fluorescence spectroscopy combined with machine learning to diagnose AD using blood plasma samples from 230 individuals (83 AD patients from 147 healthy controls). To evaluate the performance of the classification algorithms, we calculated the commonly used figures of merit (accuracy, sensitivity and specificity) and figures of merit that take into account the samples unbalance and the discrimination power of the models, as F2-score (F2), Matthews correlation coefficient (MCC) and test effectiveness ($$\delta$$
δ
). The classification models achieved satisfactory results: Parallel Factor Analysis with Quadratic Discriminant Analysis (PARAFAC-QDA) with 83.33% sensitivity, 100% specificity, 86.21% F2; and Tucker3-QDA with 91.67% sensitivity, 95.45% specificity and 91.67% F2. In addition, the classifiers show high overall performance with 94.12% accuracy and 0.87 MCC. Regarding the discrimination power between healthy and AD patients, the classification algorithms showed high effectiveness with the mean scores separated by three or more standard deviations. The PARAFAC's spectral profiles and the wavelength values from both models loading profiles can be used in future research to relate this information to plasma AD biomarkers. Our results point to a rapid, low-cost and minimally invasive blood-based method for AD diagnosis.