bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGENALAN DAN SOSIALISASI MICROSOFT PROJECT SEBAGAI SEBAGAI SARANA PENINGKATAN KOMPETENSI MANAJEMEN PROYEK DI RT 004 TEBET TIMUR DALAM
Authors: Aditya Herliawan  , Mohamad Firdaus
Published: November 2024

This community service activity was conducted in RT 004, Tebet Timur Dalam, with the aim of introducing and promoting Microsoft Project as a tool to enhance project management skills. Microsoft Project is software that facilitates efficient project planning, management, and evaluation. The implementation methods included material delivery, discussions, and practical simulations using simple case studies relevant to the participants' needs. The results of this activity demonstrated that participants gained a basic understanding of project management concepts and fundamental skills in using Microsoft Project. This training is expected to enhance the community's ability to design and manage projects, whether for personal or communal purposes, thereby supporting more structured and effective activity management.
ADOÇÃO DO ACORDO DE NÃO PERSECUÇÃO PENAL NO RIO GRANDE DO NORTE (ANPP)
Authors: Oliveira Gutemberg França de , Braga Eldo Josoé , Barroso Vivian
Published: November 2024

The Non-Prosecution Agreement (ANPP), established by Law No. 13.964/2019 (Anti-Crime Package), aims to reduce mass incarceration, especially for less serious crimes, primarily those committed negligently. This article evaluates the effectiveness of the ANPP in the Criminal Courts of the State of Rio Grande do Norte, analyzing the reduction in the number of cases, the impact on recidivism, and the social repercussions. Between 2020 and 2023, the ANPP prevented the imprisonment of more than 8,000 people, promoting a more agile justice system and consequently alleviating prison overcrowding in the state. However, despite the advances brought by the law, it is necessary to consider limitations regarding the application of this benefit to repeat offenders, particularly regarding retroactivity to the fact and the individual. As liberty is the most valuable legal good that the law seeks to guarantee and uphold, the article highlights constitutional principles such as subsidiarity and proportionality in the defense of a more rational and humane criminal policy.
AVALIAÇÃO DO NÍVEL DE CONHECIMENTO SOBRE TRANSTORNO DO ESPECTRO AUTISTA (TEA) EM ACADÊMICOS DE FISIOTERAPIA DE UM CENTRO UNIVERSITÁRIO DE TERESINA-PI
Authors: Soares Camila Barbosa , Silva Marcelia Maisa Pinheiro da , Xavier Christiane Lopes
Published: November 2024

INTRODUÇÃO: O Transtorno do Espectro Autista (TEA) é um distúrbio do neuro desenvolvimento que se manifesta pela deficiência na interação social, na comunicação e por padrões de comportamento repetitivos e estereotipados, relacionados a causas biológicas. A falta de conhecimento dos acadêmicos sobre a temática pode influenciar diretamente no diagnóstico e no tratamento dessa patologia. MÉTODOS: Estudo do tipo transversal, descritivo e quantitativo. A coleta de dados será realizada por meio do questionário  Knowledge about Chilldhood Autismo among Health Workers (KCAHW) - traduzido, que avalia o nível de conhecimento sobre Autismo. RESULTADOS: A pesquisa sobre o nível de conhecimento de acadêmicos de fisioterapia sobre transtorno do espectro autista (TEA) revelam que ainda existem lacunas no conhecimento dos estudantes de graduação de fisioterapia acerca do TEA, mesmo que o resultado da pesquisa tenha sido positivo com os alunos entrevistados, percebe-se uma falha e muita desinformação sobre o assunto.
Stem Cell Patentability: Legal Challenges
Authors: Riaz Saba , Sadiqa Ayesha , Zara Rabia , Hassan Mudassir , Hussain Ijaz , Adem Sevki , Rawat Ravi
Published: November 2024

Stem cells are versatile cells capable of self-renewal and differentiation into different specialized cell types. Their immense potential has led to a surge in research and practical applications medicine. Patentability of the stem cells has been a subject of controversy, with concerns that restrictions on access to crucial technologies could hinder the translation of research into practical medical applications. It is a promising field that's rapidly advancing and has the potential to revolutionize modern medicine. The patentability of stem cells faces several legal challenges, including ethical, moral, and religious concerns, as well as difficulties in obtaining a patent license. Patent protection is crucial for driving innovation and commercial success in regenerative medicine and life sciences. This article examines the patentability restrictions and specific challenges associated with human stem cell patents in Europe and the United States.
The Basmati rice war between India and Pakistan
Authors: Islam Sidra , Rehman Shazia , Munir Mamoona
Published: November 2024

The current study highlights the challenges for GI protection of trans-border products, in Basmati rice. Various international treaties, e.g., Lisbon Agreement, Paris convention, and TRIPS Agreement describe partly the protection of geographical indications (GIs) or appellations of origin. Both India and Pakistan are TRIPS member countries and claim Basmati rice as their GI and have tagged Basmati as GI in their respective territories. Europe is an expanding market for Basmati, and EU is implementing import regulations on food products based on GI reputation for high quality products. India has sought GI protection of Basmati rice in EU followed by staunch opposition by Pakistan. Current study will briefly highlight the recent developments of this GI dispute on Basmati rice between India and Pakistan in EU,New Zealand and Australia.
Biological nematicides as an alternative for control of Meloidogyne incognita populations in yellow pitahaya (Sselenicereus megalanthus).
Authors: Vargas Yadira , Pico Jimmy , Manobanda Neiver , Garcia Angel , Sanmiguel Jessica
Published: November 2024

Yellow pitahaya in the Ecuadorian Amazon has become one of the most important economic crops in the region. However, pests (nematodes) in the soil have affected up to 100% of the crop's growth stages. Faced with this problem, growers use various chemical nematicides that minimize this impact but cause contamination problems. For this reason, the objective of the research was to evaluate microorganisms that control or reduce the population of Meloidogyne incognita in the pitahaya crop at the greenhouse level. The design was DBCA, and the statistical analysis was performed with the statistical package Infostat 2017, using linear mixed models and Fisher's tests at 5%. The results show that root nodules decrease when P. lilacinum + T. asperellum is applied after nematode injection (261). In addition, the lowest number of nodulations (251) was obtained when microorganisms were applied after nematode inoculation (251 nodulations). Still, when microorganisms were used before, aerial biomass growth was stimulated (384.17 g) even when nematodes were present in the root system.
Keywords: microorganisms; nematodes; pitahaya.
Análisis multitemporal de deforestación y cambio de la cobertura del suelo, en el cantón La Joya de los Sachas, período 1990-2018.
Authors: Paredes-Arcos Fernando , Tinoco-Jaramillo Leider , Congo-Yépez Carlos , Vargas-Tierras Yadira
Published: November 2024

La Joya de los Sachas, es uno de los principales cantones productores de petróleo y productos agrícolas; sin
embargo, el incremento de la frontera agrícola ha ocasionado cambios importantes en la cobertura y uso del
suelo. Ante esta situación, el estudio tuvo la finalidad de conocer las causas y efectos que ha ocasionado el
cambio de cobertura para obtener las tasas anuales e índices de cambio de cobertura. El análisis se realizó
mediante el geoprocesamiento de información geográfica disponible en el portal del Ministerio de Ambiente
Agua y Transición Ecológica (MAATE) y el Instituto Geográfico Militar (IGM), se utilizó el método de tablas
de tabulación cruzada y sobreposición de capas para determinar el contraste de las coberturas entre las fechas
establecidas. Los resultados muestran un incremento acumulado de 17.626,5 ha de tierra agrícola y 16.767,6
ha de pasto en los últimos 28 años; las actividades agropecuarias se han convertido en la principal causa de
deforestación. Durante el período 1990-2018 se perdieron 36.413 ha de bosques, con una tasa anual de deforestación de 1.300 ha*año-1. Este comportamiento permitió estimar si la tendencia persiste, en 30 años aproximadamente La Joya de los Sachas perderá completamente el bosque. Además, las políticas públicas, dolarización, apertura de caminos y cambios en la actividad agrícola causaron impactos en las coberturas y usos
de suelo durante los tres periodos evaluados (1990-2000, 2000-2008 y 2008-2018).
Palabras Clave: Suelo, Uso de suelo, cobertura, tierra agrícola, pastizal, bosque, mapas.
Analysis of the feelings in the reviews of patients versus the evaluation of the ease of use, effectiveness, and satisfaction of prescribed medications
Authors: Agudelo-Santos Carlos , Zablah Jose Isaac
Published: November 2024

The sentimental polarity of patients' medical treatments is decisive for therapeutic adherence, especially in
managing chronic diseases. Patients value the effects of medications differently, while health personnel do it
from a practical perspective. For this, patient reviews have been taken in unstructured text to the diversity of
drugs available on the WebMD site. A numerical assessment accompanies these data on a Likert scale of the
variables for "EaseofUse", "Effectiveness," and "Satisfaction". Using an NLP model called RoBERTa; the
opinions have been analyzed, finding that neutral opinions are maintained against positive scales of "EaseofUse" and "Effectiveness," but negative opinions regarding "Satisfaction," where the evaluations are divided
into the extremes. The analysis has been done statistically using frequencies and diagrams of pairs between
feelings and variables of interest.
Keywords: Natural language processing; sentiment analysis; BERT; medication satisfaction
Analysis of energy poverty in Andean rural households and design of an efficient cooking solution using biomass
Authors: Silva-Castelo Wladimir A , Córdova-Uvidia Rafael , Ordoñez-Echeverria Ángel , Aquino-Arroba Miguel
Published: November 2024

This work explores energy poverty in the rural households surrounding the Chimborazo Faunal Production
Reserve (RPFCH) and proposes an efficient cooking solution using firewood. Energy poverty was evaluated
through the multidimensional poverty index (MEPI), including the 10% rule, hidden energy poverty, disproportionate expenses, and a subjective analysis of housing conditions. The research found ten households experiencing moderate energy poverty with a MEPI of 0,1562. An efficient cooking solution was proposed as
an improved rocket stove design with an ignition system and forced air at the combustion chamber inlet. This
stove utilizes a solar panel and batteries to power the fan. Its thermal efficiency is 13%, including Peltier cells
to convert residual heat into DC electricity. The rocket stove's heating capacity is 1,6 kW, which is sufficient
for heating a small room and cooking meals. After implementation, a follow-up energy poverty analysis using
the same indicators is recommended to evaluate the effectiveness of the proposed solution and its impact on
reducing energy poverty.
Keywords: energy poverty; rocket stove; thermal efficiency; room heating; Peltier cell; photovoltaic energy.
Incidence of Vibrio parahaemolyticus Coryphaena Hippurus (dorado fish) and Thunnus alalunga (albacore fish) sold in high-demand markets in Guayaquil City
Authors: Hidalgo Aura , León Melissa , Salazar Llorente Enrique , Maridueña- Zavala María Gabriela , Cevallos Cevallos Juan
Published: November 2024

Foodborne diseases affect around 600 million people in the world. In Ecuador, the Ministry of Public Health
(MSP) reported 12,203 cases of food poisoning caused mainly by Salmonella species, hepatitis virus or other pathogenic microorganisms in 2019. However, there are no reports of diseases caused by Vibrio species
in Ecuador. The present study assessed the presence of V. parahaemolyticus, mesophilic aerobics, total coliforms and E. coli. Samples of Coryphaena hippurus (dorado fish) and Thunnus alalunga (albacore fish) in 3
different markets in the city of Guayaquil, Ecuador. As a result, Vibrio parahaemolyticus was not found in
any of the 60 samples analyzed. In contrast, the E. coli levels met the requirements established in the INEN
183 - 2013 Standard norm. All samples from Market 1 were within the acceptance range regarding mesophilic aerobic levels. However, high mesophilic aerobic levels were obtained in 60% of the albacore samples
and 50% of the dorado samples from this market. In the same way, 40% of the albacore and 100% of Dorado samples from market 3 were high in mesophilic microorganisms. However, the mesophilic aerobic
analyses revealed safer levels in samples from market 1 than in markets 2 and 3, according to the NTE
INEN. 183:2013.
Keywords: Foodborne diseases, fish, Albacore, E coli, mesophilic aerobics, Dorado.
Phytochemical analysis underlying membrane stabilization and antioxidant promising potentials of Acacia nilotica seed extract.
Authors: El-Mahmoudy Abubakr
Published: November 2024

This study has aimed to evaluate the Acacia nilotica seed extract as a natural antioxidant and membrane stabilizer, as well as screening phytochemicals contained in the extract that may underline these activities. Antioxidant activity was evaluated using Phospho-molybdenum and DPPH antioxidant assays; membrane stabilization was evaluated using HRBC-MS assay, whereas phytochemicals were detected using qualitative analytical tests. Acacia nilotica hydro-methanolic extract exhibited potent, concentration (4-512 μg/mL)-dependent
antioxidant activity indicated by Phospho-molybdenum and DPPH antioxidant assays, with IC50 values of
100.5 and 33.19 μg/mL, respectively. In addition, the extract exerted membrane stabilizing effects in a concentration (4-512 μg/mL)-dependent manner, with an IC50 value of 41.47 μg/mL. Phytochemical analysis of
the plant extract clarified the active biological constituent(s) underlying these effects, including tannins, saponins, gums, flavonoids, anthraquinone glycosides, carbohydrates and proteins. These data may suggest Acacia nilotica extract, according to in vitro assays, as a highly potent antioxidant and membrane stabilizer derived from nature and could be applied as an adjunct therapy in disease conditions associated with inflammation and oxidative stress.
Keywords: Acacia nilotica; antioxidant; membrane stabilizer; anti-inflammatory.
Trust in the Workplace: Understanding How our Brains Make Decisions to Build Effective Organizational Leadership
Authors: Westover Jonathan
Published: November 2024

Abstract: This article examines the neurological bases of trust and how an awareness of unconscious trust processes can help leaders foster trusting relationships and high-performing teams. On a basic level, the brain's socioemotional circuitry generates rapid, automatic trust judgments when assessing new people or situations. Regions like the amygdala, insula, and orbitofrontal cortex evaluate factors like similarity, attractiveness, emotional expressions, body language, and facial features to determine trustworthiness outside of conscious control. While these reflexive processes conferred an evolutionary advantage, they can misfire or introduce biases in modern contexts if not guided by higher-level reasoning. The article discusses how implicit biases around these factors can impact trust dynamics. It also outlines techniques leaders can use to strategically manage first impressions and behaviors over time - through consistency, competency, care, and relationship building - to establish trust at both implicit and explicit levels. Understanding the interplay between conscious and unconscious trust judgments allows for optimizing organizational culture.
An Analysis of the Impact of Climate Changes on International Trade Patterns
Authors: Taj Urwa-tul-Wusqa
Published: November 2024

The paper aims to describe how climate change can affect the international trade. The increase in temperature owing to climate change can result in a significant decrease in trade volume, resulting in an economic loss for the country. It provides analysis of the impacts of climate change on international trade patterns. Increased temperature due to climate change can result in increased trade cost. It can also disrupt the infrastructure corridors which are vital for ensuring smooth trade. Increased concentrations of carbon dioxide due to use of harmful gases may decrease the yield of major commodity crops such as corn, rice and oats. Agricultural impacts of climate change on international trade can, in fact, raise global concerns of food security. The paper further points out potential trade implications for Pakistan owing to climate change. The Climate induced disasters such as floods can destroy Pakistan’s transport infrastructure. The recent catastrophic floods of 2022 damaged more than 8000 miles (13,000 kms) of road infrastructure, affecting the delivery of goods and services. Reduced agriculture exports will increase Pakistan’s import dependency. Another major great implication would be on Pakistan’s maritime sector. Climate change poses a direct threat to the Sea Ports which can greatly affect the trade volume. At the end, the paper also suggests recommendations to counter the threats posed by climate change and increase country’s trade volume. Role of Government in effective policy making is of paramount importance in this regard. Shift towards renewable energy resources will help in getting rid off greenhouse gases which contribute to climate change. Pakistan also needs to switch towards Sustainable Transport System. Climate change Mitigation and Adaptation Strategies, Sustainable Development, implementation of integrated policies, among others, will prove beneficial in dealing with climate change and help in boosting trade potential of Pakistan.
Doing Content/Form
Authors: Andersen Christian Ulrik , Cox Geoff
Published: November 2024

Content cannot be separated from the forms through which it is rendered. If our attachment to standardised forms and formats – served to us by big tech – limit the space for political possibility and collective action, then we ask what alternatives might be envisioned, including for research itself? What does research do in the world, and how best to facilitate meaningful intervention with attention to content and form? Perhaps what is missing is a stronger account of the structures that render our research experiences, that serve to produce new imaginaries, new spatial and temporal forms?
Between the Archive and the Feed
Authors: Palankasova Bilyana
Published: November 2024

This article discusses feminist performance and internet art practices of the 21st century through the lens of Boris Groys’s theory of innovation. It analyses works by Signe Pierce, Molly Soda, and Maya Man, to position practices of self-documentation online in exchange with feminist art histories of performance and electronic media. The text proposes that the discussed contemporary art practices fulfil the process of innovation detailed by Groys through a process of re-valuation of values via an exchange between the everyday, trivial, and heterogenous realm of social media (‘the profane’) and the valorised realm of cultural memory (‘the archive’). Using digital ethnography and contextual analysis, framed by the theory of innovation, the text introduced ‘content value’ as a feature of contemporary art on the Internet. The article demonstrates how feminist internet art practices expand on cultural value through the realisation of a process of innovation via an intra-cultural exchange between the feed and the institution.
The Autophagic Mode of Production
Authors: Cacini Luca
Published: November 2024

This article delves into the autophagic nature of generative AI in content production and its implications for cultural and technological landscapes, defined in the paper as technocene. From a broader perspective, it proposes a metabolic characterization of the technocene and explores the idea of how generative AI, such as large language models (LLMs) like ChatGPT and DALL-E, resembles an autophagic organism, akin to the biological processes of self-consumption and self- optimization. The article draws parallels between this process and cybernetics, then evokes the mythological symbol of Ouroboros, reflecting on the integration of opposites and the shadow phenomena in LLMs. Specifically, the article discusses the concepts of “Model Collapse”, "Shadow Prompting" and "Shadow Alignment," highlighting the potential for subversion and the generation of potentially harmful, rebellious content by LLMs. It also addresses the ethical implications of generative AI in art and culture, highlighting the risk of a media monoculture, the spread of disinformation and the emergence of a category of Hackers embracing methodologies to deviate these infrastructures. The discourse aims to emphasize the subversive forms of synthetic media that the process of Generative AI, embedded by repetition in the algorithmic model of the machine, may engender. By examining the autophagic nature of generative AI and its potential ethical and cultural ramifications, the article seeks to analyze the reterritorializing of the relations of production by humans in the context of content creation and consumption.
Logics of War
Authors: Lauritzen Dias Marie Naja
Published: November 2024

As manifested in Jean Baudrillard’s notoriously provoking claim that “the Gulf War did not take place,” mediatization of war has long been associated with illusion. Today, war images that circulate online are increasingly judged by their proximity to ‘truth,’ eliciting a skepticism towards their ‘evidentiary’ value. By juxtaposing Baudrillard’s reading of the mediatization of the Gulf War with the contemporary image theories of e.g. Cecilia Sjöholm and Matthew Fuller and Eyal Weizman, the article explores how this skepticism is expressed in a contemporary context. Through visual analysis of a YouTube video of a press conference held at the bombed Al-Ahli Baptist Hospital in Gaza, it examines the relationship between the f orm through which the war is perceived (the images) and their cont ent (the ‘realities’ of war). Through a lens oered by Georges Didi-Huberman, the article concludes by suggesting that by expanding what I term the snapshot logic of war images to embrace a scenography of war, the press conference video gives form to the condition of desperation and suering in Gaza.
Xeno-Tuning
Authors: Rizo-Casado Esther
Published: November 2024

Xenoimage Dataset is an artistic practice that unleashes the hallucinatory capacities of image-generating AI to question the perpetuation of power dynamics inherent in normative gender dichotomies. Employing techniques called xeno-tuning, it adapts pre-trained models to produce weird representations of corporealities, criticising the homogenous tendencies and biases inherent in image datasets. The purpose is to define the visuality of the xeno as a transformative agent of current hegemonic identities.
Studies in vegetation and soil of coastal salt marshes in the disko area, West Greenland.
Authors: Vestergaard Peter
Published: November 2024

At seven localities along the coasts of Disko and Nugssuaq the vegetation and soil conditions of salt marshes have been analysed by means of gradient analyses. In a number of sample plots along transects, which represent the change in vegetation with increasing elevation, the species composition was quantified by frequency and cover determinations, soil samples were taken at depths of 0-5 and 5-15 cm, and the relative elevation was established as the height of the plot above the lower salt marsh limit in percentage of the vertical range of the salt marsh. The number of species pr. m• in each plot and the species similarity between theplots along each transect were calculated. The soil samples were analysed for actual water content, cation exchange capacity, organic matter, specific conductivity, pH, water-extractable and NH,Acextractable Ca, Mg, K and Na, water-extractable Cl, H2S04-extractable P and EDT A-extractable Cu, Pb, Zn and Ni. From these data a number of derived soil data were computed. From the soil data in total, mean and standard deviation for each soil factor within six classes of relative elevation, and correlation coefficients between the soil factors mutual and between the soil factors and relative elevation, were computed. The vegetation and soil conditions of each locality are described, and the general trends in the vegetation structure and edaphic conditions of the salt marshes in relation to the height above the lower salt marsh limit, which seems to correspond to the mean high water level, are pointed out. It is shown, that the salt marshes form ecoclines from lower to upper marsh limit, composed by vegetational and environmental gradients. The ecocline has been divided into a lower and an upper salt marsh, dominated by Puccinellia phryganodes and Carex glareosa respectively; the vegetational and environmental characteristics of these marsh parts are described.
The efficiency of using a heat pump in the construction of buildings on sites with a sunken roof of icy permafrost
Authors: Gorbunova Alina , Gunar Alexey , Ozeritskiy Konstantin Vladimirovich , Balihin Ermolai
Published: November 2024

Currently, there is a problem of reliability of buildings and structures built on permafrost soils according to principle I, which is due to the dependence of standard design solutions on the climatic component. The solution to this problem involves the introduction of methods of active thermal stabilization of soils, which makes it possible to effectively manage heat flows and ensure the preservation of the base soils in a frozen state. One of the key solutions proposed by the authors is the use of a heat pump with the placement of a cooling circuit in a soil massif. The use of heat pumps in construction on sites with permafrost soils is an innovative approach that ensures the safety and efficiency of building operation. In the article, using a specific example of a building in Salekhard, the possibility of using a heat pump on a site with a sunken roof of icy permafrost rocks is considered.Research methods include the analysis and generalization of literary sources and multivariate computational experiments to study the temperature field of the building foundation soils using a heat pump. The main conclusion of the study is the expediency of using heat pumps in the construction of buildings in areas with a sunken roof of icy permafrost. The innovative method proposed in this article for preserving the soils of the base in a frozen state, when applied in practice, will prove to be an effective solution against the background of a new natural challenge — global climate warming. In addition to keeping the foundation soils in a frozen state and ensuring stable operation of buildings and structures in the cryolithozone, heat pumps provide significant economic benefits by reducing operating costs. Heat pumps also contribute to reducing emissions of carbon dioxide and other pollutants, which is important in the context of global climate change and the pursuit of sustainable development.
Inclusive, Exclusive and Hierarchical Atlas of NFATc1+/PDGFR-α+ Cells in Dental and Periodontal Mesenchyme
Authors: Yang Xue , Han Chuyi , Yu Changhao , Zhou Bin , Ye Ling , Li Feifei , Yu Fanyuan
Published: November 2024

Platelet-derived growth factor receptor alpha (PDGFR-α) activity is crucial in the process of dental and periodontal mesenchyme regeneration facilitated by autologous platelet concentrates (APCs), such as platelet-rich fibrin (PRF), platelet-rich plasma (PRP) and concentrated growth factors (CGF), as well as by recombinant PDGF drugs. However, it is largely unclear about the physiological patterns and cellular fate determinations of PDGFR-α
+
cells in the homeostasis maintaining of adult dental and periodontal mesenchyme. We previously identified NFATc1 expressing PDGFR-α
+
cells as a subtype of skeletal stem cells (SSCs) in limb bone, but their roles in dental and periodontal remain unexplored. To this end, in the present study we investigated the spatiotemporal atlas of NFATc1
+
and PDGFR-α
+
cells residing in dental and periodontal mesenchyme, their capacity for progeny cell generation, and their inclusive, exclusive and hierarchical relations in homeostasis. We utilized CRISPR/Cas9-mediated gene editing to generate two dual recombination systems, which were
Cre/loxP
-
Dre/Rox
combined intersectional and exclusive reporters respectively, to concurrently demonstrate the inclusive, exclusive, and hierarchical distributions of NFATc1
+
and PDGFR-α
+
cells and their lineage commitment. By employing the state-of-the-art transgenic lineage tracing techniques in cooperating with tissue clearing-based advanced imaging and three-dimensional slices reconstruction, we systematically mapped the distribution atlas of NFATc1
+
and PDGFR-α
+
cells in dental and periodontal mesenchyme and tracked their
in vivo
fate trajectories. Our findings extend current understanding of NFATc1
+
and PDGFR-α
+
cells in dental and periodontal mesenchyme homeostasis, and furthermore enhance our comprehension of their sustained therapeutic impact for future clinical investigations.
PDZ-directed substrate recruitment is the primary determinant of specific 4E-BP1 dephosphorylation by PP1-Neurabin
Authors: Fedoryshchak Roman O , El-Bouri Karim , Joshi Dhira , Mouilleron Stephane , Treisman Richard
Published: November 2024

Protein Phosphatase 1 (PP1) relies on association with PP1-interacting proteins (PIPs) to generate substrate-specific PIP/PP1 holoenzymes, but the lack of well-defined substrates has hindered elucidation of the mechanisms involved. We previously demonstrated that the Phactr1 PIP confers sequence specificity on the Phactr1/PP1 holoenzyme by remodelling the PP1 hydrophobic substrate groove. Phactr1 defines a group of “RVxF-ΦΦ-R-W” PIPs that all interact with PP1 in a similar fashion. Here we use a PP1-PIP fusion approach to address sequence specificity and identify substrates of the RVxF-ΦΦ-R-W family PIPs. We show that the four Phactr proteins confer identical sequence specificities on their holoenzymes. We identify the 4E-BP and p70 S6K translational regulators as substrates for the Neurabin/Spinophilin PIPs, implicated in neuronal plasticity, pointing to a role for their holoenzymes in mTORC1-dependent translational control. Biochemical and structural experiments show that in contrast to the Phactrs, substrate recruitment and catalytic efficiency of the PP1-Neurabin and PP1-Spinophilin fusions is primarily determined by substrate interaction with the PDZ domain adjoining their RVxF-ΦΦ-R-W motifs, rather than by recognition of the remodelled PP1 hydrophobic groove. Thus, even PIPs that interact with PP1 in a similar manner use different mechanisms to ensure substrate selectivity.
Spatial and temporal distribution of ribosomes in single cells reveals aging differences between old and new daughters of Escherichia coli
Authors: Chao Lin , Chan Chun Kuen , Shi Chao , Rang Ulla Camilla
Published: November 2024

Lineages of rod-shaped bacteria such as Escherichia coli exhibit a temporal decline in elongation rate in a manner comparable to cellular or biological aging. The effect results from the production of asymmetrical daughters, one with a lower elongation rate, by the division of a mother cell. The slower daughter compared to the faster daughter, denoted respectively as the old and new daughters, has more aggregates of damaged proteins and fewer expressed gene products. We have examined further the degree of asymmetry by measuring the density of ribosomes between old and new daughters and between their poles. We found that ribosomes were denser in the new daughter and also in the new pole of the daughters. These ribosome patterns match the ones we previously found for expressed gene products. This outcome suggests that the asymmetry is not likely to result from properties unique to the gene expressed in our previous study, but rather from a more fundamental upstream process affecting the distribution of ribosomal abundance. Because damage aggregates and ribosomes are both more abundant at the poles of E. coli cells, we suggest that competition for space between the two could explain the reduced ribosomal density in old daughters. Using published values for aggregate sizes and the relationship between ribosomal number and elongation rates, we show that the aggregate volumes could in principle displace quantitatively the amount of ribosomes needed to reduce the elongation rate of the old daughters.
Ab initio prediction of specific phospholipid complexes and membrane association of HIV-1 MPER antibodies by multi-scale simulations
Authors: Maillie Colleen , Golden Jay , Wilson Ian A , Ward Andrew B , Mravic Marco
Published: November 2024

A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein’s membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. We address the feasibility and determinant molecular features of this mechanism using multi-scale modeling. All-atom simulations of 4E10, PGZL1, 10E8 and LN01 docked onto HIV-like membranes consistently form phospholipid complexes at key complementarity-determining region loop sites, solidifying that stable and specific lipid interactions anchor bnAbs to membrane surfaces. Ancillary protein-lipid contacts reveal surprising contributions from antibody framework regions. Coarse-grained simulations effectively capture antibodies embedding into membranes. Simulations estimating protein-membrane interaction strength for PGZL1 variants along an inferred maturation pathway show bilayer affinity is evolved and correlates with neutralization potency. The modeling demonstrated here uncovers insights into lipid participation in antibodies’ recognition of membrane proteins and highlights antibody features to prioritize in vaccine design.
Genetic stability of Mycobacterium smegmatis under the stress of first-line antitubercular agents
Authors: Molnár Dániel , Surányi Éva Viola , Trombitás Tamás , Füzesi Dóra , Hirmondó Rita , Toth Judit
Published: November 2024

The sustained success of Mycobacterium tuberculosis as a pathogen arises from its ability to persist within macrophages for extended periods and its limited responsiveness to antibiotics. Furthermore, the high incidence of resistance to the few available antituberculosis drugs is a significant concern, especially since the driving forces of the emergence of drug resistance are not clear. Drug-resistant strains of Mycobacterium tuberculosis can emerge through de novo mutations, however, mycobacterial mutation rates are low. To unravel the effects of antibiotic pressure on genome stability, we determined the genetic variability, phenotypic tolerance, DNA repair system activation, and dNTP pool upon treatment with current antibiotics using Mycobacterium smegmatis. Whole-genome sequencing revealed no significant increase in mutation rates after prolonged exposure to first-line antibiotics. However, the phenotypic fluctuation assay indicated rapid adaptation to antibiotics mediated by non-genetic factors. The upregulation of DNA repair genes, measured using qPCR, suggests that genomic integrity may be maintained through the activation of specific DNA repair pathways. Our results, indicating that antibiotic exposure does not result in de novo adaptive mutagenesis under laboratory conditions, do not lend support to the model suggesting antibiotic resistance development through drug pressure-induced microevolution.
Motor neurons are dispensable for the assembly of a sensorimotor circuit for gaze stabilization
Authors: Goldblatt Dena , Rosti Basak , Hamling Kyla Rose , Leary Paige , Panchal Harsh , Li Marlyn , Gelnaw Hannah , Huang Stephanie , Quainoo Cheryl , Schoppik David
Published: November 2024

Sensorimotor reflex circuits engage distinct neuronal subtypes, defined by precise connectivity, to transform sensation into compensatory behavior. Whether and how motor neuron populations specify the subtype fate and/or sensory connectivity of their pre-motor partners remains controversial. Here, we discovered that motor neurons are dispensable for proper connectivity in the vestibular reflex circuit that stabilizes gaze. We first measured activity following vestibular sensation in pre-motor projection neurons after constitutive loss of their extraocular motor neuron partners. We observed normal responses and topography indicative of unchanged functional connectivity between sensory neurons and projection neurons. Next, we show that projection neurons remain anatomically and molecularly poised to connect appropriately with their downstream partners. Lastly, we show that the transcriptional signatures that typify projection neurons develop independently of motor partners. Our findings comprehensively overturn a long-standing model: that connectivity in the circuit for gaze stabilization is retrogradely determined by motor partner-derived signals. By defining the contribution of motor neurons to specification of an archetypal sensorimotor circuit, our work speaks to comparable processes in the spinal cord and advances our understanding of principles of neural development.
Correlation between pre-operative VE-cadherin and DLL4 and the maturation after primary arteriovenous fistula in uremic patients
Authors: Yin Huanhuan , Tang Yifan , Wang Yanping , Waheed Yousuf Abdulkarim , Wang Disheng , Sun Dong
Published: November 2024

Aims

Uremic patients require dialysis to replace the declined kidney function, and arteriovenous fistula (AVF) is a commonly used dialysis access route. Our study aimed to explore vascular endothelial cells cadherin (VE-cadherin) and Delta-like ligand 4 (DLL4) expression in uremic patients undergoing primary AVF surgery and their correlation with AVF maturation.

Methods

We conducted a prospective study that included n = 55 voluntary uremic patients receiving their initial AVF procedure for renal replacement therapy, subjects were divided into a mature group and a failure group based on whether the AVF matured within 3 months post-operatively. We analyzed the association of VE-cadherin and DLL4 with AVF maturation by examining their expression levels in serum and the endothelium of cephalic veins.

Results

Pre-operative serum VE-cadherin, in the mature group measured 125.07 (106.77–167.65) ng/L, and DLL4 was 92.78 (83.83–106.72) pg/mL, while the failure group had VE-cadherin at 95.40 (79.03–107.16) ng/L (P = 0.001), and DLL4 at 60.42 (43.98–80.15) pg/mL with a statistical significant; (P = 0.002), binary logistic regression analysis indicated a significant association between cephalic vein diameter, VE-cadherin, DLL4 levels, and AVF immaturity (P = 0.024, P = 0.014 respectively). Immunohistochemical staining showed slightly higher VE-cadherin levels in the mature group than in the failure group (P = 0.366). DLL4 was primarily located in the cell membrane and cytoplasm, concentrated in the inner membrane, with significantly higher levels in the mature group compared to the failure group (P = 0.027).

Conclusion

The failure group exhibited lower levels of VE-cadherin and DLL4 in serum and vascular tissue, these results suggest that VE-cadherin and DLL4 might play pivotal regulatory roles in the onset and the progression of fistula immaturity, potentially serving as promising targets for future interventions.
Exploração da influência dos parâmetros no arco secundário durante manobras de religamento monopolar
Authors: Sandmann André , Sandmann Ana Flavia Maldaner Teodoro , Schutz Fabiana Costa Araujo , Santos Jose Airton Azevedo dos , Hallal Renato , Portolan Marcos Roberto , Pelegrini Ricardo Luiz , Cadini Larissa , Melges André Inácio , Bressan Rodrigo Techio
Published: November 2024

O comportamento do arco secundário em linhas de transmissão durante manobras de religamento monopolar é um dos fatores mais críticos para a confiabilidade dos sistemas elétricos de alta tensão. Esse fenômeno, sustentado por correntes induzidas entre os condutores e o potencial de terra, pode comprometer a restauração do serviço de energia caso não seja extinto adequadamente. Este artigo apresenta uma análise abrangente da literatura científica, com foco nos últimos dez anos, sobre os parâmetros que influenciam a formação, sustentação e extinção do arco secundário. O estudo explora avanços em modelagem matemática, simulações computacionais e soluções tecnológicas que contribuem para mitigar os efeitos adversos do arco, bem como o impacto de fatores operacionais, como características dos isoladores, geometria dos condutores e condições ambientais. Além disso, destaca a utilização de sistemas inteligentes e aprendizado de máquina como ferramentas promissoras para prever e controlar esse fenômeno em redes complexas. A revisão evidencia lacunas no conhecimento atual e propõe direções para novas investigações, com o objetivo de desenvolver estratégias mais eficientes e sustentáveis para a operação de linhas de transmissão. Este trabalho oferece uma contribuição significativa para a melhoria da segurança, estabilidade e desempenho de sistemas elétricos modernos.
The Effects of Additional Tuna Madihang Bone Fish Treatment on the Growth of Catfish (Clarias gariepinus) in Biofloc Pond in Ternate City North Maluku, Indonesia
Authors: Talib Ahmad , Noh Muhammad Hi
Published: November 2024

The need for fish protein always increases along with the increase in urban population. To meet the food needs of the people in the city of Ternate, one way is to cultivate catfish using a biofloc system. To accelerate the growth of catfish, one of the efforts made is by adding fish bone meal to standard feed. Tuna fish bone meal contains minerals, especially calcium and phosphorus, which can help accelerate the growth of catfish so that harvest time can be accelerated. The objectives of the research are (1) To introduce catfish cultivation with a biofloc system that is effective, efficient and easy to do even on limited land (2) Fish bone meal addition treatment with fish bone meal concentrations (0%, 10%, 15% and 20%) and (3) The catfish cultivation system using the biofloc method can provide maximum results. The method used in this research is an experimental method on a biofloc pond system which consists of three biofloc ponds with a diameter of 3x3 meters. The results of this research are (1) Catfish is a fishery product that has an important prospect for development through cultivation using biofloc ponds (2) The best average value was in the treatment of adding fish bone meal with a concentration of 20% in almost all treatments except for the growth rate treatment which was highest in treatment (c) 0.73 and (3) The addition of tuna bone meal to biofloc pond cultivation can be used as a recommendation for developing catfish cultivation because it has quite promising market prospects.
Application of Curriculum Mapping in Relation to Classroom Learning Outcomes of Public Elementary Schools
Authors: Miano Mary Shielo D. , Baguio Josephine B.
Published: November 2024

This study was conducted to determine the relationship between the application of curriculum mapping and classroom learning outcomes of public elementary schools in South District of Governor Generoso, Division of Davao Oriental. This utilized the non-experimental quantitative research design employing correlational method. The respondents were composed of teachers using universal sampling. The statistical tools used were Mean, Pearson Product Moment Coefficient Correlation (Pearson r) and Regression Analysis. The application of curriculum mapping and classroom learning outcomes in public elementary schools is oftentimes manifested by the teachers. There is a significant relationship between application of curriculum mapping and classroom learning outcomes in public elementary schools. The domains of analysis on the application of curriculum mapping indicators significantly influence classroom learning outcomes indicators. Thus, the application of curriculum mapping in relation to learning outcomes should be strengthened in school to help the teachers recognize the school vision, arm them with the motivation and tools to achieve it and compensate them appropriately for their efforts by being aware of teachers need and treating everyone fairly, to find that employees will be more motivated to do their work.
Preoperatively Manifestations as Univesicular Mass-like Lesion and Postoperatively as a Multivesicular Lesion with Multiple Daughter Cysts: A Very Rare Case Report
Authors: Aghajanzadeh Manouchehr , Foumani Ali Alavi , Tangestaninejad Azita , Mosavi Seyed Aliaskhar Fakher , Rad Omid Mosaffaee , Sadin Zahra , Farzin Mohaya
Published: November 2024

Pulmonary vesicular hydatid cysts and uncomplicated cysts with echinococcosis granulose and daughter cysts are very rare. A 58-year-old man presented to our center with cough, hemoptysis, and right-side chest wall pain. Chest x-ray and computerized tomography scan revealed a cystic-like appearance mass in the hill of the right lung. FOB and BAL were normal. With right side PL- --thoracotomy, the cystic mass of the lung was removed. Hundreds of 1to3 cm size daughter cysts and laminated membrane was discovered after opened the cystic mass. Pathologic examinations revealed the hydatid cysts with daughter cysts on the lung cystic mass. To the best of our knowledge, this is probably a rare presentation of a lung hydatid cyst as a mass-like and univesicular cyst, which is full of a daughter cyst.
Influence of INM on Yield, Quality of Potato and Soil Status
Authors: Raval C. H. , Satodiya B. N. , Pardva D. R. , Patel A. J.
Published: November 2024

An experiment was carried out to study the Influence of INM on yield, quality of potato and soil status at Horticulture Research Farm, College of Horticulture, AAU, Anand during the three consecutive years 2020-21, 2021-22 and 2022-23. The experiment was laid out in Randomized Block Design with three replications and ten treatments viz., T1: 100 % RDF (220:110:220), T2: 20 t FYM + 100 % RDF (220:110:220), T3: 75 % RDF + 25 % RDN through FYM, T4: 75 % RDF + 25 % RDN through VC, T5: 50 % RDF + 50 % RDN through FYM, T6: 50 % RDF + 50 % RDN through VC, T7: 50 % RDF + 50 % RDN through FYM + 1 L Bio NPK consortium/ha, T8: 50 % RDF + 50 % RDN through VC + 1 L Bio NPK consortium/ha, T9: 25 % RDF + 50 % RDN through FYM + 1 L Bio NPK consortium/ha + KMB 1 L/ha, T10: 25 % RDF + 50 % RDN through VC + 1 L Bio NPK consortium + KMB 1 L/ha. The treatment T2 [20 t FYM + 100 % RDF (220:110:220] recorded maximum tuber weight per plant (487.13 g), tuber yield (434.8 q/ha) and grade “A” tuber (378.9 q/ha) in pooled analysis. Treatments T7 to T10 received inoculation of Bio NPK consortium showed higher microbial population. Whereas, grade “B” tuber, TSS and soil status after harvest were found non-significant in pooled data.