bluuebunny/crossref_metadata_2025 huggingface dataset:
Protection the rights of minority communities in the era of religious freedom
Authors: Ghifari Farhan
Published: June 2021

Islamic religious education, including subjects that must be given to students who are Muslim, even though these students study at non-Islamic schools. Likewise, on the other hand, Islamic schools must also facilitate religious education in accordance with the religions of their students. Santo Paulus Catholic High School Jember, including a school that facilitates Islamic religious education for Muslim students. Uniquely, there is a religiosity subject, which includes all universal values in the official religion in Indonesia. The focus of this research is, how is the portrait and dynamics of Islamic religious education in non-Muslim schools (Catholic schools)? In this context, the authors chose a qualitative approach in data mining and processing. Interviews, document studies, observation, data research are the techniques chosen in research data collection. The research findings: 1) SMA Catholic Santo Paulus Jember has 6 Islamic religious education teachers, but they are not in accordance with the qualifications of the subjects they are teaching, 2) apart from PAI subjects, SMA Catholic Santo Paulus Jember strengthens students with religiosity lessons, namely lessons which includes universal values of all religions, 3) SMA Catholic Santo Paulus Jember is in demand by students from the six official religions in Indonesia.
Identifikasi dan Karakterisasi Aktivitas Ekstrak Kasar Enzim Lipase Isolat Bakteri Lipolitik Lptk 19 Asal Tempe Biji Karet
Authors: Simamora Cico Jhon Karunia , Sukmawati Sukmawati
Published: June 2021

Lipase adalah enzim yang larut dalam air yang memiliki kemampuan untuk menghidrolisis triasilgliserol untuk melepaskan asam lemak bebas dan gliserol. Penelitian ini bertujuan untuk mengidentifikasi dan mengkarakterisasi aktivitas ekstrak kasar enzim lipase. Pelaksanaan penelitian meliputi peremajaan isolat, pengujian kualitatif produksi lipase yang ditunjukkan dengan adanya zona bening di sekitar koloni pada medium LAOR, dan dilanjutkan dengan pengujian kuantitatif aktivitas enzim lipase, uji aktivitas spesifik enzim, dan identifikasi biokimia isolat bakteri LpTk 19.Â  Penelitian ini menunjukkan hasil uji kualitatif bakteri LpTK 19 pada media LAOR yang diinkubasi selama 48 jam mencapai 90,59%. Sementara nilai aktivitas enzim tertinggi didapatkan padaÂ  jam ke-60 sebesar 487,5 U/mL, kadar protein tertinggi pada jam ke-48 sebesar 12,4 mg/mL, dan aktivitas spesifik tertinggi berada pada jam ke-60 sebesar 325,0 U/mg. Berdasarkan uji biokimia diketahui isolate bakteri LpTk 19 termasuk dalam golongan bakteri Gram positif, yang termasuk family Bacillaceae, dengan jenis Bacillus sp.Â
REVIEW OF SOFTWARE TOOLS FOR GENETIC MUTATION ANALYSIS
Authors: Chidibere Onuora Augustine , Emmanuel Maidoh Nonye , Oko Epia Godwin , Ana Prince , Ana Prince , Ana Prince
Published: June 2021

Genes are basic physical and functional unit of
heredity. Genes are made up of DNA. Some genes act as
instructions to make molecules called proteins. However,
many genes do not code for proteins. Gene Mutation refers
to modification of gene’s DNA sequence. Mutation of
genes can vary within a genome and between genomes.
Mutations range in size; they can affect anywhere from a
single DNA building block (base pair) to a large segment of
a chromosome that includes multiple genes. Because
mutation is one of the fundamental forces of evolution, this
work looked into the various computer software tools that
can be used for genetic mutation analysis. These
applications were reviewed stating the OS platform it
depends on, the nature and format of data it accept, the
input and output from these software tools and urls were
they can be found were also stated.
EXPLORING THE POTENTIALS OF ARTIFICIAL INTELLGIENCE IN THE JUDICIARY
Authors: Samson Abiodun Oloruntoba , John Lekan Akinode
Published: June 2021

In recent years, there has been massive
progress in Artificial Intelligence (AI) with the
development of machine learning, deep neural networks,
natural language processing, computer vision and robotics.
These techniques are now actively being applied in the
judiciary with many of the legal service activities currently
being delivered by lawyers predicted to be taken over by
AI in the coming years. This paper explores the potentials
and efficiency of Artificial intelligence (AI) in justice
delivery. The paper has two objectives: first to highlight
the main applications of AI in justice administrations
through some examples of AI tools recently developed;
second, to assess the ethical challenges of AI in the
judiciary. Artificial Intelligence algorithms are starting to
support lawyers, for instance, through artificial
intelligence search tools, or to support justice
administrations with predictive technologies and business
analytics based on the computation of Big Data. Using the
concept of Artificial Intelligence (AI), Legal knowledgebased tools may accelerate the service delivery of legal
professionals from typical searching of related case
journals to extraction of precise information in a
customized manner.
Religious Rivalry in the Seventeenth Century: A Buddhist–Christian Case in China
Authors: Fu Amy Yu
Published: June 2021

To shed new light on the place of Christianity in seventeenth-century Chinese society and the debates and conflicts between Christians and Buddhists, this paper reflects on Christians' critiques of Buddhist dogma and praxis as well as rejoinders from the Buddhists. It will focus on the sustained debates, roughly between 1590 and 1690, with regard to the relative ‘merits and defects’ as represented in polemical texts. Several treatises serve as the essential link of the continuous debates, eliciting back-and-forth elaboration and rebuttals from both sides. Through an analysis of the polemical discourse, I argue that the Buddhist–Christian case offers an instance of what René Girard termed ‘mimetic rivalry’. The conflict entails internal rivalry resulting not only from different religious perspectives but also from social, cultural and economic ones. Seeking interconnectedness between traditions by creative imagination and analogy may offer a way out of ignorance and enmity in dealing with interreligious relationship. 1
The Scientific Calling of the Church: Herman Bavinck's Exhortation for the Churches in Mainland China
Authors: Xu Ximian
Published: June 2021

Herman Bavinck (1854–1921) describes the twofold scientific calling of the Church. First, the Church needs to read the historic Reformed confessions contextually and distill the Reformed principles to meet its contemporary needs. Second, the Church should pursue a scientific ( wetenschappelijke) life, particularly in the university. Bavinck's twofold theological insight can be applied to the churches in mainland China. The first reminds Chinese Reformed churches of the necessity of composing a Sino-Reformed confession. The second insight exhorts churches to develop scientific life publicly. In this sense, the scientific calling of the church, which Bavinck envisaged more than a century before, can be fulfilled in the twenty-first-century mainland China. 1
Endothelial Aryl Hydrocarbon Receptor Nuclear Translocator Mediates the Angiogenic Response to Peripheral Ischemia in Mice With Type 2 Diabetes Mellitus
Authors: Nguyen Tu , Zheng Mei , Knapp Maura , Sladojevic Nikola , Zhang Qin , Ai Lizhuo , Harrison Devin , Chen Anna , Sitikov Albert , Shen Le , Gonzalez Frank J. , Zhao Qiong , Fang Yun , Liao James J. K. , Wu Rongxue
Published: June 2021

Hypoxia-inducible factors (HIFs) are the master regulators of angiogenesis, a process that is impaired in patients with diabetes mellitus (DM). The transcription factor aryl hydrocarbon receptor nuclear translocator (ARNT, also known as HIF1β) has been implicated in the development and progression of diabetes. Angiogenesis is driven primarily by endothelial cells (ECs), but both global and EC-specific loss of ARNT-cause are associated with embryonic lethality. Thus, we conducted experiments in a line of mice carrying an inducible, EC-specific ARNT-knockout mutation (ArntΔEC, ERT2) to determine whether aberrations in ARNT expression might contribute to the vascular deficiencies associated with diabetes. Mice were first fed with a high-fat diet to induce diabetes. ArntΔEC, ERT2 mice were then adminstrated with oral tamoxifen to disrupt Arnt and peripheral angiogenesis was evaluated by using laser-Doppler perfusion imaging to monitor blood flow after hindlimb ischemia. The ArntΔEC, ERT2 mice had impaired blood flow recovery under both non-diabetic and diabetic conditions, but the degree of impairment was greater in diabetic animals. In addition, siRNA-mediated knockdown of ARNT activity reduced measurements of tube formation, and cell viability in human umbilical vein endothelial cells (HUVECs) cultured under high-glucose conditions. The ArntΔEC, ERT2 mutation also reduced measures of cell viability, while increasing the production of reactive oxygen species (ROS) in microvascular endothelial cells (MVECs) isolated from mouse skeletal muscle, and the viability of ArntΔEC, ERT2 MVECs under high-glucose concentrations increased when the cells were treated with an ROS inhibitor. Collectively, these observations suggest that declines in endothelial ARNT expression contribute to the suppressed angiogenic phenotype in diabetic mice, and that the cytoprotective effect of ARNT expression in ECs is at least partially mediated by declines in ROS production.
Eco-Friendly Synthesis, Biological Evaluation, and In Silico Molecular Docking Approach of Some New Quinoline Derivatives as Potential Antioxidant and Antibacterial Agents
Authors: El-Saghier Ahmed M. , El-Naggar Mohamed , Hussein Abdel Haleem M. , El-Adasy Abu-Bakr A. , Olish M. , Abdelmonsef Aboubakr H.
Published: June 2021

A new series of quinoline derivatives 5–12 were efficiently synthesized via one-pot multicomponent reaction (MCR) of resorcinol, aromatic aldehydes, β-ketoesters, and aliphatic/aromatic amines under solvent-free conditions. All products were obtained in excellent yields, pure at low-cost processing, and short time. The structures of all compounds were characterized by means of spectral and elemental analyses. In addition, all the synthesized compounds 5–12 were in vitro screened for their antioxidant and antibacterial activity. Moreover, in silico molecular docking studies of the new quinoline derivatives with the target enzymes, human NAD (P)H dehydrogenase (quinone 1) and DNA gyrase, were achieved to endorse their binding affinities and to understand ligand–enzyme possible intermolecular interactions. Compound 9 displayed promising antioxidant and antibacterial activity, as well as it was found to have the highest negative binding energy of -9.1 and -9.3 kcal/mol for human NAD (P)H dehydrogenase (quinone 1) and DNA gyrase, respectively. Further, it complied with the Lipinski’s rule of five, Veber, and Ghose. Therefore, the quinoline analogue 9 could be promising chemical scaffold for the development of future drug candidates as antioxidant and antibacterial agents.
Analytical and Clinical Evaluation of “AccuPower SARS-CoV-2 Multiplex RT-PCR kit (Bioneer, South Korea)” and “Allplex 2019-nCoV Assay (Seegene, South Korea)” for SARS-CoV-2 RT-PCR Diagnosis: Korean CDC EUA as a Quality Control Proxy for Developing Countries
Authors: Freire-Paspuel Byron , Garcia-Bereguiain Miguel Angel
Published: June 2021

Background

Multiple RT-qPCR kits are available in the market for SARS-CoV-2 diagnosis, some of them with Emergency Use Authorization (EUA) by FDA or their country of origin agency, but many of them lack of proper clinical evaluation.

Objective

We evaluated the clinical performance of two Korean SARS-CoV-2 RT-PCR kits available in South America, AccuPower SARS-CoV-2 Multiplex RT-PCR kit (Bioneer, South Korea) and Allplex 2019-nCoV Assay (Seegene, South Korea), for RT-qPCR SARS-CoV-2 diagnosis using the CDC protocol as a gold standard.

Results

We found strong differences among both kits clinical performance and analytical sensitivity; while the Allplex 2019-nCoV Assay has sensitivity of 96.5% and an estimated limit of detection of 4,000 copies/ml, the AccuPower SARS-CoV-2 Multiplex RT-PCR kit has a sensitivity of 75.5% and limit of detection estimated to be bigger than 20,000 copies/ml.

Conclusions

AccuPower SARS-CoV-2 Multiplex RT-PCR kit and Allplex 2019-nCoV Assay are both made in South Korea but EUA by Korean CDC was only granted to the later. Our results support that Korean CDC EUA should be considered as a quality control proxy for Korean SARS-CoV-2 RT-PCR kits prior to importation by developing countries to guarantee high sensitivity diagnosis.
Remote Renewable Hubs for Carbon-Neutral Synthetic Fuel Production
Authors: Berger Mathias , Radu David , Detienne Ghislain , Deschuyteneer Thierry , Richel Aurore , Ernst Damien
Published: June 2021

This paper studies the economics of carbon-neutral synthetic fuel production from renewable electricity in remote areas where high-quality renewable resources are abundant. To this end, a graph-based optimisation modelling framework directly applicable to the strategic planning of remote renewable energy supply chains is proposed. More precisely, a hypergraph abstraction of planning problems is introduced, wherein nodes can be viewed as optimisation subproblems with their own parameters, variables, constraints and local objective. Nodes typically represent a subsystem such as a technology, a plant or a process. Hyperedges, on the other hand, express the connectivity between subsystems. The framework is leveraged to study the economics of carbon-neutral synthetic methane production from solar and wind energy in North Africa and its delivery to Northwestern European markets. The full supply chain is modelled in an integrated fashion, which makes it possible to accurately capture the interaction between various technologies on an hourly time scale. Results suggest that the cost of synthetic methane production and delivery would be slightly under 150 €/MWh (higher heating value) by 2030 for a system supplying 10 TWh annually and relying on a combination of solar photovoltaic and wind power plants, assuming a uniform weighted average cost of capital of 7%. A comprehensive sensitivity analysis is also carried out in order to assess the impact of various techno-economic parameters and assumptions on synthetic methane cost, including the availability of wind power plants, the investment costs of electrolysis, methanation and direct air capture plants, their operational flexibility, the energy consumption of direct air capture plants, and financing costs. The most expensive configuration (around 200 €/MWh) relies on solar photovoltaic power plants alone, while the cheapest configuration (around 88 €/MWh) makes use of a combination of solar PV and wind power plants and is obtained when financing costs are set to zero.
Anaerobic Mesophilic Co-Digestion of Swine Slurry and Hidrolyzate in Batch Reactors: A Case Study
Authors: Domingues Paulo S. , Pala Helena , Oliveira Nelson S.
Published: June 2021

The management of residual flows from swine farms, such as slurry and hydrolyzate, is a serious environmental problem in Portugal, so the search for new solutions is important, especially if they can be implemented in production sites, avoiding the transport of waste. Anaerobic co-digestion can configure a sustainable method of management and valorization of these flows in swine farms, resulting in biogas to produce energy and a digestate with agronomic value. The swine hydrolyzate is the product from the elimination of swine carcasses on the farms, through a hydrolysis process. Its use in the anaerobic process wasn’t found in the literature, identifying the need to evaluate its potential. Thus, anaerobic co-digestion of swine slurry and hydrolyzate was carried out and the main purpose of this research was to find the best feeding ratio between the two substrates in batch test, focusing on biogas production. The study was developed in two phases, both under mesophilic conditions. In the first one, using 100 ml syringes, the effect of the relative proportion of substrates on the anaerobic co-digestion potential was investigated and the effect of pressure sterilization of the substrates on biogas production was verified. In the second phase, a larger scale study was carried out in a 4,500 ml digestor, ran under the conditions identified as the most favorable in the preliminary tests. The results obtained allowed us to conclude that the largest volume of accumulated biogas was obtained with the proportion of 90.9% swine slurry: 9.1% swine hydrolyzate (v/v); and that the sterilization of the substrates doesn’t constitute an effective thermal pre-treatment. The larger scale test revealed an inefficient anaerobic process due to the inhibitory effect caused by the accumulation of ammonia and volatile organic acids. However, the production yield was 606.8 LBiogas/kgVS and 431.6 LMethane/kgVS, indicating that process allowed a production of biogas and methane higher than the values cited in literature for anaerobic digestion of swine slurry. This result showed that the use of swine hydrolyzate as a co-substrate results in a better balance of nutrients, promoting a better development of microorganisms.
Responses of Soil Microbial Biomass and Enzyme Activities to Natural Restoration of Reclaimed Temperate Marshes After Abandonment
Authors: Wang Chunguang , Li Haixing , Sun Xiaoxin , Cai Tijiu
Published: June 2021

Restoration of reclaimed marshes has great effects on soil biological processes. However, the responses of soil microbial properties (microbial biomass and enzyme activities) to natural restoration of reclaimed marshes is poorly studied, especially in a long restoration chronosequence. This study assessed the responses of soil microbial properties to natural restoration and investigated the relationships between soil microbial properties and soil physico-chemical and plant properties. We selected a restoration chronosequence (1, 4, 8, 13, 17, 27 years) after farmland abandonment, a soybean field, and a natural marsh in Sanjiang Plain, northeast China. For each site, we analyzed the soil microbial biomass carbon and nitrogen (MBC and MBN), four enzymes (β-glucosidase, invertase, catalase, urease) activities, soil physico-chemical properties at 0–50 cm depths, and plant properties (biomass, height, and coverage). The MBC and MBN contents increased with restoration time, but MBN content slowed down after 8 years of restoration. After 27 years of restoration, the soil MBC and MBN contents were 15.7 and 3.2 times of those in the soybean field, but the largest contents of MBC and MBN in the restored sites were 7.78%, 27.76% lower than those in natural marshes, respectively. Moreover, soil enzyme activities and the geometric mean of enzymatic activities (GME) also increased with restoration but slowed down after 13 years of restoration. After 27 years of restoration, the GME was 2.9 times than that in the soybean field, but the largest GME in the restored sites was 31.15% lower than that in the natural marsh. MBC and MBN contents, soil enzyme activities, and GME had significant relationships with soil C:N ratio, organic carbon, nutrients (total nitrogen, available nitrogen, total phosphorus), bulk density, moisture content, pH, plant properties, (i.e. biomass, height, and coverage) (p < 0.01). Redundancy analysis revealed that soil C:N ratio, pH, moisture content, total nitrogen and phosphorus were main factors affecting MBC and MBN contents and enzyme activities. In conclusion, soil microbial properties can respond positively to the natural restoration process of the reclaimed marshes and were significantly correlated with specific parameters of soil physico-chemical and plant properties.
Factor D Inhibition Blocks Complement Activation Induced by Mutant Factor B Associated With Atypical Hemolytic Uremic Syndrome and Membranoproliferative Glomerulonephritis
Authors: Aradottir Sigridur Sunna , Kristoffersson Ann-Charlotte , Roumenina Lubka T. , Bjerre Anna , Kashioulis Pavlos , Palsson Runolfur , Karpman Diana
Published: June 2021

Complement factor B (FB) mutant variants are associated with excessive complement activation in kidney diseases such as atypical hemolytic uremic syndrome (aHUS), C3 glomerulopathy and membranoproliferative glomerulonephritis (MPGN). Patients with aHUS are currently treated with eculizumab while there is no specific treatment for other complement-mediated renal diseases. In this study the phenotype of three FB missense variants, detected in patients with aHUS (D371G and E601K) and MPGN (I242L), was investigated. Patient sera with the D371G and I242L mutations induced hemolysis of sheep erythrocytes. Mutagenesis was performed to study the effect of factor D (FD) inhibition on C3 convertase-induced FB cleavage, complement-mediated hemolysis, and the release of soluble C5b-9 from glomerular endothelial cells. The FD inhibitor danicopan abrogated C3 convertase-associated FB cleavage to the Bb fragment in patient serum, and of the FB constructs, D371G, E601K, I242L, the gain-of-function mutation D279G, and the wild-type construct, in FB-depleted serum. Furthermore, the FD-inhibitor blocked hemolysis induced by the D371G and D279G gain-of-function mutants. In FB-depleted serum the D371G and D279G mutants induced release of C5b-9 from glomerular endothelial cells that was reduced by the FD-inhibitor. These results suggest that FD inhibition can effectively block complement overactivation induced by FB gain-of-function mutations.
Detailed Multiplex Analysis of SARS-CoV-2 Specific Antibodies in COVID-19 Disease
Authors: Brynjolfsson Siggeir F. , Sigurgrimsdottir Hildur , Einarsdottir Elin D. , Bjornsdottir Gudrun A. , Armannsdottir Brynja , Baldvinsdottir Gudrun E. , Bjarnason Agnar , Gudlaugsson Olafur , Gudmundsson Sveinn , Sigurdardottir Sigurveig T. , Love Arthur , Kristinsson Karl G. , Ludviksson Bjorn R.
Published: June 2021

A detailed understanding of the antibody response against SARS-CoV-2 is of high importance, especially with the emergence of novel vaccines. A multiplex-based assay, analyzing IgG, IgM, and IgA antibodies against the receptor binding domain (RBD), spike 1 (S1), and nucleocapsid proteins of the SARS-CoV-2 virus was set up. The multiplex-based analysis was calibrated against the Elecsys® Anti-SARS-CoV-2 assay on a Roche Cobas® instrument, using positive and negative samples. The calibration of the multiplex based assay yielded a sensitivity of 100% and a specificity of 97.7%. SARS-CoV-2 specific antibody levels were analyzed by multiplex in 251 samples from 221 patients. A significant increase in all antibody types (IgM, IgG, and IgA) against RBD was observed between the first and the third weeks of disease. Additionally, the S1 IgG antibody response increased significantly between weeks 1, 2, and 3 of disease. Class switching appeared to occur earlier for IgA than for IgG. Patients requiring hospital admission and intensive care had higher levels of SARS-CoV-2 specific IgA levels than outpatients. These findings describe the initial antibody response during the first weeks of disease and demonstrate the importance of analyzing different antibody isotypes against multiple antigens and include IgA when examining the immunological response to COVID-19.
Mapping Spatial Distribution and Biomass of Intertidal Ulva Blooms Using Machine Learning and Earth Observation
Authors: Karki Sita , Bermejo Ricardo , Wilkes Robert , Monagail Michéal Mac , Daly Eve , Healy Mark , Hanafin Jenny , McKinstry Alastair , Mellander Per-Erik , Fenton Owen , Morrison Liam
Published: June 2021

Graphical Abstract

Overall research workflow showing data types, study area, model development and biomass results.
Current Status of Forecasting Toxic Harmful Algae for the North-East Atlantic Shellfish Aquaculture Industry
Authors: Fernandes-Salvador Jose A. , Davidson Keith , Sourisseau Marc , Revilla Marta , Schmidt Wiebke , Clarke Dave , Miller Peter I. , Arce Paola , Fernández Raúl , Maman Luz , Silva Alexandra , Whyte Callum , Mateo Maria , Neira Patricia , Mateus Marcos , Ruiz-Villarreal Manuel , Ferrer Luis , Silke Joe
Published: June 2021

Across the European Atlantic Arc (Scotland, Ireland, England, France, Spain, and Portugal) the shellfish aquaculture industry is dominated by the production of mussels, followed by oysters and clams. A range of spatially and temporally variable harmful algal bloom species (HABs) impact the industry through their production of biotoxins that accumulate and concentrate in shellfish flesh, which negatively impact the health of consumers through consumption. Regulatory monitoring of harmful cells in the water column and toxin concentrations within shellfish flesh are currently the main means of warning of elevated toxin events in bivalves, with harvesting being suspended when toxicity is elevated above EU regulatory limits. However, while such an approach is generally successful in safeguarding human health, it does not provide the early warning that is needed to support business planning and harvesting by the aquaculture industry. To address this issue, a proliferation of web portals have been developed to make monitoring data widely accessible. These systems are now transitioning from “nowcasts” to operational Early Warning Systems (EWS) to better mitigate against HAB-generated harmful effects. To achieve this, EWS are incorporating a range of environmental data parameters and developing varied forecasting approaches. For example, EWS are increasingly utilizing satellite data and the results of oceanographic modeling to identify and predict the behavior of HABs. Modeling demonstrates that some HABs can be advected significant distances before impacting aquaculture sites. Traffic light indices are being developed to provide users with an easily interpreted assessment of HAB and biotoxin risk, and expert interpretation of these multiple data streams is being used to assess risk into the future. Proof-of-concept EWS are being developed to combine model information with in situ data, in some cases using machine learning-based approaches. This article: (1) reviews HAB and biotoxin issues relevant to shellfish aquaculture in the European Atlantic Arc (Scotland, Ireland, England, France, Spain, and Portugal; (2) evaluates the current status of HAB events and EWS in the region; and (3) evaluates the potential of further improving these EWS though multi-disciplinary approaches combining heterogeneous sources of information.
Deposition Characteristics of Firebrands on and Around Rectangular Cubic Structures
Authors: Mankame Aditya , Shotorban Babak
Published: June 2021

The focus of the present work is on the deposition of firebrands in a flow over a rectangular cubic block representative of a structure in wildland-urban interface (WUI). The study was carried out by physics based modeling where the wind flow turbulence was dealt with by large eddy simulation (LES) and firebrands were treated by Lagrangian tracking. The Lagrangian equations coupled with the flow solver, accounted for both translational and rotational motions as well as thermochemical degradation of firebrands, assumed to be cylindrical. The dimensions of the structure were varied from 3 to 9 m in the simulations for a parametric study. The simulations were carried out by tracking many firebrands randomly released with a uniform distribution from a horizontal plane 35 m above the ground into the computational domain. The coordinates of the deposited firebrands were used to calculate their normalized number density (number of landed firebrands per unit surface area) to quantify their deposition pattern. On the leewardside of the block, an area, referred to as the safe zone, was identified right behind the structure where firebrands never deposit. The size of the safe zone in the direction perpendicular to the wind was nearly identical to the width of the structure. The length of the safe zone in the wind direction was proportional to the height of the structure. The leeward face of the blocks was never hit by a firebrand. The windward face was hit by many more firebrands than the lateral faces but much less than the top face. The distribution of the number density of the deposited firebrands on the top face was found to be correlated with the flow separation and reattachment on this face.
A Personal Respirator to Improve Protection for Healthcare Workers Treating COVID-19 (PeRSo)
Authors: Elkington Paul T. , Dickinson Alexander S. , Mavrogordato Mark N. , Spencer Daniel C. , Gillams Richard J. , De Grazia Antonio , Rosini Sebastian , Garay-Baquero Diana J. , Diment Laura E. , Mahobia Nitin , Mant Alexandra , Baynham Tom , Morgan Hywel
Published: June 2021

Introduction: SARS-CoV-2 infection is a global pandemic. Personal Protective Equipment (PPE) to protect healthcare workers has been a recurrent challenge in terms of global stocks, supply logistics and suitability. In some settings, around 20% of healthcare workers treating COVID-19 cases have become infected, which leads to staff absence at peaks of the pandemic, and in some cases mortality.

Methods: To address shortcomings in PPE, we developed a simple powered air purifying respirator, made from inexpensive and widely available components. The prototype was designed to minimize manufacturing complexity so that derivative versions could be developed in low resource settings with minor modification.

Results: The “Personal Respirator – Southampton” (PeRSo) delivers High-Efficiency Particulate Air (HEPA) filtered air from a battery powered fan-filter assembly into a lightweight hood with a clear visor that can be comfortably worn for several hours. Validation testing demonstrates that the prototype removes microbes, avoids excessive CO2 build-up in normal use, and passes fit test protocols widely used to evaluate standard N95/FFP2 and N99/FFP3 face masks. Feedback from doctors and nurses indicate the PeRSo prototype was preferred to standard FFP2 and FFP3 masks, being more comfortable and reducing the time and risk of recurrently changing PPE. Patients report better communication and reassurance as the entire face is visible.

Conclusion: Rapid upscale of production of cheaply produced powered air purifying respirators, designed to achieve regulatory approval in the country of production, could protect healthcare workers from infection and improve healthcare delivery during the COVID-19 pandemic.
Antimicrobial Peptides Derived From Insects Offer a Novel Therapeutic Option to Combat Biofilm: A Review
Authors: Sahoo Alaka , Swain Shasank Sekhar , Behera Ayusman , Sahoo Gunanidhi , Mahapatra Pravati Kumari , Panda Sujogya Kumar
Published: June 2021

Biofilms form a complex layer with defined structures, that attach on biotic or abiotic surfaces, are tough to eradicate and tend to cause some resistance against most antibiotics. Several studies confirmed that biofilm-producing bacteria exhibit higher resistance compared to the planktonic form of the same species. Antibiotic resistance factors are well understood in planktonic bacteria which is not so in case of biofilm producing forms. This may be due to the lack of available drugs with known resistance mechanisms for biofilms. Existing antibiotics cannot eradicate most biofilms, especially of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species). Insects produce complex and diverse set of chemicals for survival and defense. Antimicrobial peptides (AMPs), produced by most insects, generally have a broad spectrum of activity and the potential to bypass the resistance mechanisms of classical antibiotics. Besides, AMPs may well act synergistically with classical antibiotics for a double-pronged attack on infections. Thus, AMPs could be promising alternatives to overcome medically important biofilms, decrease the possibility of acquired resistance and treatment of multidrug-resistant pathogens including ESKAPE. The present review focuses on insect-derived AMPs with special reference to anti-biofilm-based strategies. It covers the AMP composition, pathways and mechanisms of action, the formation of biofilms, impact of biofilms on human diseases, current strategies as well as therapeutic options to combat biofilm with antimicrobial peptides from insects. In addition, the review also illustrates the importance of bioinformatics tools and molecular docking studies to boost the importance of select bioactive peptides those can be developed as drugs, as well as suggestions for further basic and clinical research.
Characterization of Stormwater Runoff Based on Microbial Source Tracking Methods
Authors: Monteiro Silvia , Queiroz Gaspar , Ferreira Filipa , Santos Ricardo
Published: June 2021

Rainfall and associated urban runoff have been linked to an increased deterioration of environmental waters, carrying several pollutants including pathogenic microorganisms. Such happens because fecal matter is washed into storm drainage pipes that are afterward released into environmental waters. Stormwater has not been extensively characterized as it is, because most studies are performed either on drainage pipes that are often impacted by sewage leakage or directly in environmental waters following a rain event. In this study, stormwater collected directly from the streets, was monitored for the presence of fecal indicator bacteria (FIB) and three potential important sources of fecal contamination in urban environments (human, cats, and dogs) in three distinct basins in Lisbon, Portugal. Stormwater was collected in sterilized plastic boxes inserted in the storm drains, therefore collecting only runoff. High concentration of fecal contamination was detected with a high percentage of the samples displayed at least one source of contamination. A strong relationship was found between the number of detected sources and the precipitation levels. Although no statistical correlation was found between the locations and the presence of FIB or source markers, the results show a trend in geographical information on the type of urban use in each basin. To the best of our knowledge, this is the first study analyzing the runoff collected directly from the streets. This study suggests that, in urban areas, stormwater runoff is highly impacted by fecal matter, not only from domestic animals but also from human origin, before any cross-contamination in the drainage system and may, by itself, pose a high risk to human health and the environment, particularly if water reuse of this water without further disinfection treatment is the final goal.
CORO1C is Associated With Poor Prognosis and Promotes Metastasis Through PI3K/AKT Pathway in Colorectal Cancer
Authors: Wang Zongxia , Jia Lizhou , sun Yushu , Li Chunli , Zhang Lingli , Wang Xiangcheng , Chen Hao
Published: June 2021

Trophoblast cell surface protein 2 (Trop2) is one of the cancer-related proteins that plays a vital role in biological aggressiveness and poor prognosis of colorectal cancer (CRC). The study of the Trop2 related network is helpful for us to understand the mechanism of tumorigenesis. However, the effects of the related proteins interacting with Trop2 in CRC remain unclear. Here, we found that coronin-like actin-binding protein 1C (CORO1C) could interact with Trop2 and the expression of CORO1C in CRC tissues was higher than that in paracarcinoma tissues. The expression of CORO1C was associated with histological type, lymph node metastasis, distant metastasis, AJCC stage, venous invasion, and perineural invasion. The correlation between CORO1C expression and clinical characteristics was analyzed demonstrating that high CORO1C expression in CRC patients were associated with poor prognosis. Furthermore, CORO1C knockdown could decrease the cell proliferation, colony formation, migration and invasion in vitro and tumor growth in vivo. The underlying mechanisms were predicted by bioinformatics analysis and verified by Western blotting. We found that PI3K/AKT signaling pathway was significantly inhibited by CORO1C knockdown and the tuomr-promoting role of CORO1C was leastwise partly mediated by PI3K/AKT signaling pathway. Thus, CORO1C may be a valuable prognostic biomarker and drug target in CRC patients.
Memristor Circuits for Simulating Neuron Spiking and Burst Phenomena
Authors: Innocenti Giacomo , Di Marco Mauro , Tesi Alberto , Forti Mauro
Published: June 2021

Since the introduction of memristors, it has been widely recognized that they can be successfully employed as synapses in neuromorphic circuits. This paper focuses on showing that memristor circuits can be also used for mimicking some features of the dynamics exhibited by neurons in response to an external stimulus. The proposed approach relies on exploiting multistability of memristor circuits, i.e., the coexistence of infinitely many attractors, and employing a suitable pulse-programmed input for switching among the different attractors. Specifically, it is first shown that a circuit composed of a resistor, an inductor, a capacitor and an ideal charge-controlled memristor displays infinitely many stable equilibrium points and limit cycles, each one pertaining to a planar invariant manifold. Moreover, each limit cycle is approximated via a first-order periodic approximation analytically obtained via the Describing Function (DF) method, a well-known technique in the Harmonic Balance (HB) context. Then, it is shown that the memristor charge is capable to mimic some simplified models of the neuron response when an external independent pulse-programmed current source is introduced in the circuit. The memristor charge behavior is generated via the concatenation of convergent and oscillatory behaviors which are obtained by switching between equilibrium points and limit cycles via a properly designed pulse timing of the current source. The design procedure takes also into account some relationships between the pulse features and the circuit parameters which are derived exploiting the analytic approximation of the limit cycles obtained via the DF method.
MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis
Authors: Li Hailin , Han Guangyu , Li Xing , Li Bowen , Wu Bo , Jin Hongyuan , Wu Lingli , Wang Wei
Published: June 2021

Background

MAPK-RAP1A signaling, which is involved in cancer progression, remains to be defined. Upregulation of MAPK-RAP1A signaling accounts for most cancers that harbor high incident rate, such as non-small cell lung cancer (NSCLC) and pancreatic cancer, especially in hepatocellular carcinoma (HCC). MAPK-RAP1A signaling plays an important function as clinical diagnosis and prognostic value in cancers, and the role of MAPK-RAP1A signaling related with immune infiltration for HCC should be elucidated.

Methods

Microarray data and patient cohort information from The Cancer Genome Atlas (TCGA; n = 425) and International Cancer Genome Consortium (ICGC; n = 405) were selected for validation. The Cox regression and least absolute shrinkage and selection operator (LASSO) were used to construct a clinical prognostic model in this analysis and validation study. We also tested the area under the curve (AUC) of the risk signature that could reflect the status of predictive power by determining model. MAPK-RAP1A signaling is also associated with tumor-infiltrating immune cells (TICs) as well as clinical parameters in HCC. The GSEA and CIBERSORT were used to calculate the proportion of TICs, which should be beneficial for the clinical characteristics (clinical stage, distant metastasis) and positively correlated with the survival of HCC patients.

Results

HCC patients with enrichment of MAPK-RAP1A signaling were associated with clinical characteristics and favorable T cell gamma delta (Vδ T cells), and STMN1, RAP1A, FLT3, HSPA8, ANGPT2, and PGF were used as candidate biomarkers for risk scores of HCC. To determine the molecular mechanism of this signature gene association, Gene Set Enrichment Analysis (GSEA) was proposed. Cytokine–cytokine receptor interaction, TGF-β signaling pathway, and Intestinal immune network for IgA production gene sets were closely related in MAPK-RAP1A gene sets. Thus, we established a novel prognostic prediction of HCC to deepen learning of MAPK-RAP1A signaling pathways.

Conclusion

Our findings demonstrated that HCC patients with enrichment of MAPK-RAP1A signaling were associated with clinical characteristics and favorable T cell gamma delta (Vδ T cells), which may be a novel prognostic prediction of HCC.
Implemented Interventions at the Naef K. Basile Cancer Institute to Protect Patients and Medical Personnel From COVID Infections: Effectiveness and Patient Satisfaction
Authors: El Cheikh Jean , El Warrak Samantha , Ghaoui Nohra , Al Chami Farouk , Shahbaz Maya , Chehayeb Sarah , Saghir Nagi , Bazarbachi Ali , Taher Ali
Published: June 2021

Background

The Coronavirus Disease 2019 (COVID-19) was declared a pandemic by WHO in March 2020. The first case of COVID-19 was identified in Lebanon on the 21st of February 2020, amid a national economic crisis. As the numbers of cases increased, ICU admissions and mortality rose, which led hospitals across Lebanon to take certain safety measures to contain the virus. The Naef K. Basile Cancer Institute (NKBCI) at the American University of Beirut Medical Center handles oncology outpatient visits and outpatient treatment protocol infusions. The aim of this study is to evaluate the efficacy of the safety measures put forth by the NKBCI early in the pandemic.

Methods

Oncology patients are amongst the immunosuppressed population, who are at greatest risk of contracting COVID-19 and consequently suffering its complications. In this manuscript, we evaluated the precautionary measures implemented at the NKBCI of AUBMC from March 1st to May 31st of 2020, by surveying oncology patients on the telephone who had live and virtual appointments in both the oncology outpatient clinics and infusion unit. We conducted a prospective study of 670 oncology patients who had appointments at the NKBCI during this period and used their answers to draw responses about patient satisfaction towards those safety measures.

Results

Our results involved 387 responses of oncology patients who visited the NKBCI during the period of March 1st to May 31st of 2020. 99% of our respondents gave a rating of good to excellent with these new measures. The option of online consultation was given to 35% in the hematology group compared to 19% in those with solid tumors (p=0.001). From the total, 15% of patients opted for the telemedicine experience as a new implemented strategy to provide patient-centered medical care. Of this group of patients, 22% faced problems with connectivity and 19% faced problems with online payment.

Conclusion

NKBCI was competent in following the WHO guidelines in protecting the oncology patient population. Feedback collected from the surveys will be taken into account by the committee of the NKBCI to develop new safety measures that can better control viral spread while providing patient-centered medical care.
Health Measurement Model—Bringing a Life Course Perspective to Health Measurement: The PRISM Model
Authors: Hirschfeld Steven , Goodman Elizabeth , Barkin Shari , Faustman Elaine , Halfon Neal , Riley Anne W.
Published: June 2021

Health is a multidimensional concept that is challenging to measure, and in the rapidly evolving developmental changes that occur during the first 21 years of human life, requires a dynamic approach to accurately capture the transitions, and overall arc of a complex process of internal and external interactions. We propose an approach that integrates a lifecourse framework with a layered series of assessments, each layer using a many to many mapping, to converge on four fundamental dimensions of health measurement-Potential, Adaptability, Performance, and Experience. The four dimensions can conceptually be mapped onto a plane with each edge of the resulting quadrilateral corresponding to one dimension and each dimensions assessment calibrated against a theoretical ideal. As the plane evolves over time, the sequential measurements will form a volume. We term such a model the Prism Model, and describe conceptually how single domain assessments can be built up to generate the holistic description through the vehicle of a layer of Exemplar Cases. The model is theoretical but future work can use the framework and principles to generate scalable and adaptable applications that can unify and improve the precision of serial measurements that integrate environmental and physiologic influences to improve the science of child health measurement.
Repeated Aconitine Treatment Induced the Remodeling of Mitochondrial Function via AMPK–OPA1–ATP5A1 Pathway
Authors: Qiu Li-Zhen , Zhou Wei , Yue Lan-Xin , Wang Yi-Hao , Hao Fei-Ran , Li Peng-Yan , Gao Yue
Published: June 2021

Aconitine is attracting increasing attention for its unique positive inotropic effect on the cardiovascular system, but underlying molecular mechanisms are still not fully understood. The cardiotonic effect always requires abundant energy supplement, which is mainly related to mitochondrial function. And OPA1 has been documented to play a critical role in mitochondrial morphology and energy metabolism in cardiomyocytes. Hence, this study was designed to investigate the potential role of OPA1-mediated regulation of energy metabolism in the positive inotropic effect caused by repeated aconitine treatment and the possible mechanism involved. Our results showed that repeated treatment with low-doses (0–10 μM) of aconitine for 7 days did not induce detectable cytotoxicity and enhanced myocardial contraction in Neonatal Rat Ventricular Myocytes (NRVMs). Also, we first identified that no more than 5 μM of aconitine triggered an obvious perturbation of mitochondrial homeostasis in cardiomyocytes by accelerating mitochondrial fusion, biogenesis, and Parkin-mediated mitophagy, followed by the increase in mitochondrial function and the cellular ATP content, both of which were identified to be related to the upregulation of ATP synthase α-subunit (ATP5A1). Besides, with compound C (CC), an inhibitor of AMPK, could reverse aconitine-increased the content of phosphor-AMPK, OPA1, and ATP5A1, and the following mitochondrial function. In conclusion, this study first demonstrated that repeated aconitine treatment could cause the remodeling of mitochondrial function via the AMPK–OPA1–ATP5A1 pathway and provide a possible explanation for the energy metabolism associated with cardiotonic effect induced by medicinal plants containing aconitine.