bluuebunny/crossref_metadata_2025 huggingface dataset:
Critical Considerations for Investigating MicroRNAs during Tumorigenesis: A Case Study in Conceptual and Contextual Nuances of miR-211-5p in Melanoma
Authors: Vand-Rajabpour Fatemeh , Savage Meghan , Belote Rachel L. , Judson-Torres Robert L.
Published: April 2023

MicroRNAs are non-coding RNAs fundamental to metazoan development and disease. Although the aberrant regulation of microRNAs during mammalian tumorigenesis is well established, investigations into the contributions of individual microRNAs are wrought with conflicting observations. The underlying cause of these inconsistencies is often attributed to context-specific functions of microRNAs. We propose that consideration of both context-specific factors, as well as underappreciated fundamental concepts of microRNA biology, will permit a more harmonious interpretation of ostensibly diverging data. We discuss the theory that the biological function of microRNAs is to confer robustness to specific cell states. Through this lens, we then consider the role of miR-211-5p in melanoma progression. Using literature review and meta-analyses, we demonstrate how a deep understating of domain-specific contexts is critical for moving toward a concordant understanding of miR-211-5p and other microRNAs in cancer biology.
Health and Growth of Black Pine outside Its Natural Distribution Range in the Romanian Carpathians
Authors: Tudoran Gheorghe-Marian , Cicșa Avram , Dobre Alexandru-Claudiu , Cicșa Maria , Pascu Ionuț-Silviu , Leca Ştefan
Published: April 2023

In the last decade, coniferous stands outside their natural range in Romania have experienced declines in both their health and growth and, in tandem with global climate trends, these forests are becoming even more threatened. We studied the relationship between tree growth and defoliation as an indicator of tree health. The data came from black pine stands monitored from 2012 to 2021 in the Postăvarul Massif in the Romanian Carpathians. Analyses were carried out on 508 individual trees based on their defoliation and radial growth data and also at the stand level. The results revealed an increase in the percentage of tree defoliation from 17% to 38% during the studied decade, along with 13.5% tree mortality. Over the decade, radial growth showed a negative trend, driven significantly by defoliation. The biometric parameters of the trees did not influence their percentage of defoliation. In contrast, spring/summer droughts associated with high temperatures affect the health and growth of trees. Models generated from the temperature–defoliation–radial-growth relationship estimated a significant continuous reduction in the radial growth of the trees of 0.5%–0.6% for each 1% increase in defoliation. Under the site conditions of the investigated stands, an increase in basal area and stocking degree significantly increased stand defoliation. This was further accentuated when the pine stand included an understory of young trees. As a rule, in the interest of production, stands are kept dense to fully exploit the site, but thinning may become necessary to protect these stands and ensure their survival as the climate changes.
Numerical Simulation on the Smoke Prevention Performance of Air Curtains in an Island-Type Subway Station
Authors: Yan Xu , Yang Hongyun , Mo Huiqiang , Xie Ye , Jin Zhongfu , Zhou Yang
Published: April 2023

Subway fires are a major threat to the safe and smooth operation of subway stations. In this paper, an island-type subway station was taken as an example to conduct a series of numerical simulations using Fire Dynamics Simulator (FDS). The temperature, visibility, and CO concentration in the subway station were analysed under different thicknesses and jet velocities of the air curtains. The smoke-prevention performance of the air curtains in the subway station was investigated. As the thickness and jet velocity increase, the flame tilts significantly, which greatly hinders the spread of smoke toward the stairs. The smoke temperature and CO concentration on the left side of the air curtains gradually decrease, while the visibility increases significantly. For a 3 MW fire scenario, to satisfy the evaluation criteria, the results show that the thickness of the air curtains needs to be at least 0.3 m, and the jet velocity needs to be at least 2 m/s. The sealing effectiveness (Esealing) tends to increase and then remains constant with increasing momentum, and the maximum is obtained when the momentum of the air curtains (Ia) is 12.5 kg·m/s2. Meanwhile, it is found that an energy-saving efficiency of 85.2% can be achieved by replacing positive pressure ventilation with air curtains. The results of this work can provide a significant reference for the design of smoke protection in subway stations.
Identification of Mycobacterium chelonae from Lined Seahorse Hippocampus erectus and Histopathological Analysis
Authors: Bai Xiaohui , Hao Shuang , Fu Jianping , Sun Hanchang , Luo Zhang
Published: April 2023

The lined seahorse (Hippocampus erectus) is an aquacultural species in China and has important economic and ornamental value. However, the species is affected by disease, which restricts their large-scale cultivation. In 2021, a disease was observed in cultured seahorses in Tianjin, China, with a cumulative mortality rate of 20%. The symptoms observed in the moribund seahorse included a weak swimming capacity, discolored body surface, enlarged liver and kidneys, and numerous white nodules in the parenchymatous organs. The strains HM-2021-1 and HM-2021-2 were isolated from diseased seahorses and were identified as being responsible for the disease. It demonstrated the potential to infect seahorse, and the cumulative mortalities of the seahorses artificially infected with strains HM-2021-1 and HM-2021-2 were 93.3% and 90.0%. The pathogen was identified as Mycobacterium chelonae based on physiological and biochemical tests, and 16S rDNA, rpoB, and Hsp65 gene sequence analysis. To our knowledge, this study is the first report of M. chelonae associated with diseased seahorses in China.
Topological Vortexes, Asymptotic Freedom, and Multifractals
Authors: Migdal Alexander
Published: April 2023

In this paper, we study the Kelvinons, which are monopole ring solutions to the Euler equations, regularized as the Burgers vortex in the viscous core. There is finite anomalous dissipation in the inviscid limit. However, in the anomalous Hamiltonian, some terms are growing as logarithms of Reynolds number; these terms come from the core of the Burgers vortex. In our theory, the turbulent multifractal phenomenon is similar to asymptotic freedom in QCD, with these logarithmic terms summed up by an RG equation. The small effective coupling does not imply small velocity; on the contrary, velocity is large compared to its fluctuations, which opens the way for a quantitative theory. In the leading order in the perturbation theory in this effective coupling constant, we compute running multifractal dimensions for high moments of velocity circulation, which is in good agreement with the data for quantum Turbulence and available data for classical Turbulence. The logarithmic dependence of fractal dimensions on the loop size comes from the running coupling in anomalous dimensions. This slow logarithmic drift of fractal dimensions would be barely observable at Reynolds numbers achievable at modern DNS.
Bigel Formulations of Nanoencapsulated St. John’s Wort Extract—An Approach for Enhanced Wound Healing
Authors: Sotirova Yoana , Gugleva Viliana , Stoeva Stanila , Kolev Iliyan , Nikolova Rositsa , Marudova Maria , Nikolova Krastena , Kiselova-Kaneva Yoana , Hristova Minka , Andonova Velichka
Published: April 2023

This study aimed to develop a semisolid vehicle for topical delivery of nanoencapsulated St. John’s wort (SJW) extract, rich in hyperforin (HP), and explore its wound-healing potential. Four nanostructured lipid carriers (NLCs) were obtained: blank and HP-rich SJW extract-loaded (HP-NLC). They comprised glyceryl behenate (GB) as a solid lipid, almond oil (AO), or borage oil (BO) representing the liquid lipid, along with polyoxyethylene (20) sorbitan monooleate (PSMO) and sorbitan monooleate (SMO) as surfactants. The dispersions demonstrated anisometric nanoscale particles with acceptable size distribution and disrupted crystalline structure, providing entrapment capacity higher than 70%. The carrier exhibiting preferable characteristics (HP-NLC2) was gelled with Poloxamer 407 (PM407) to serve as the hydrophilic phase of a bigel, to which the combination of BO and sorbitan monostearate (SMS) organogel was added. The eight prepared bigels with different proportions (blank and nanodispersion-loaded) were characterized rheologically and texturally to investigate the impact of the hydrogel-to-oleogel ratio. The therapeutic potential of the superior formulation (HP-NLC-BG2) was evaluated in vivo on Wistar male rats through the tensile strength test on a primary-closed incised wound. Compared with a commercial herbal semisolid and a control group, the highest tear resistance (7.764 ± 0.13 N) was achieved by HP-NLC-BG2, proving its outstanding wound-healing effect.
The First Complete Chloroplast Genome of Cordia monoica: Structure and Comparative Analysis
Authors: Alshegaihi Rana M. , Mansour Hassan , Alrobaish Shouaa A. , Al Shaye Najla A. , Abd El-Moneim Diaa
Published: April 2023

Cordia monoica is a member of the Boraginaceae family. This plant is widely distributed in tropical regions and has a great deal of medical value as well as economic importance. In the current study, the complete chloroplast (cp) genome of C. monoica was sequenced, assembled, annotated, and reported. This circular chloroplast genome had a size of 148,711 bp, with a quadripartite structure alternating between a pair of repeated inverted regions (26,897–26,901 bp) and a single copy region (77,893 bp). Among the 134 genes encoded by the cp genome, there were 89 protein-coding genes, 37 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. A total of 1387 tandem repeats were detected, with the hexanucleotides class making up 28 percent of the repeats. Cordia monoica has 26,303 codons in its protein-coding regions, and leucine amino acid was the most frequently encoded amino acid in contrast to cysteine. In addition, 12 of the 89 protein-coding genes were found to be under positive selection. The phyloplastomic taxonomical clustering of the Boraginaceae species provides further evidence that chloroplast genome data are reliable not only at family level but also in deciphering the phylogeny at genus level (e.g., Cordia).
Bictegravir and Metformin Drug-Drug Interaction in People with Human Immunodeficiency Virus (HIV)
Authors: Masich Anne M. , Thompson Lindsey , Fulco Patricia P.
Published: April 2023

A drug-drug interaction (DDI) exists between bictegravir and metformin. Bictegravir inhibits renal organic cation transporter-2, leading to increased metformin plasma concentrations. The objective of this analysis was to evaluate the clinical implications of concomitant bictegravir and metformin administration. This was a retrospective, single-center, descriptive analysis evaluating people with human immunodeficiency virus (PWH) concurrently prescribed bictegravir and metformin between February 2018–June 2020. PWH lost to follow-up or non-adherent were excluded. Data collection included: hemoglobin A1C (HgbA1C), HIV RNA viral load, CD4 cell count, serum creatinine, and lactate. Adverse drug reactions (ADRs) were assessed by provider-documented, patient-reported symptoms of gastrointestinal (GI) intolerance and hypoglycemia. Metformin dose adjustments and discontinuations were recorded. Fifty-three PWH were included (116 screened; 63 excluded). GI intolerance was reported in three PWH (5.7%). There were no documented episodes of hypoglycemia or lactic acidosis. Five PWH had metformin dose reductions (N = 3 for unspecified reasons; N = 1 for GI intolerance) or discontinuation (N = 1 unrelated to ADRs). Both diabetes and HIV control improved (HgbA1C decreased by 0.7% with virologic control in 95% of PWH). Minimal ADRs were reported in PWH receiving concurrent metformin and bictegravir. Prescribers should be aware of this potential interaction; however, no empiric metformin total daily dose adjustment appears necessary.
Inter-Examiner and Intra-Examiner Reliability of Quantitative and Qualitative Ultrasonography Assessment of Peripheral and Respiratory Muscles in Critically Ill Patients
Authors: Barbosa Felipe Douglas Silva , Dos Santos José Lucas , Alves Maria Emilia Dantas , Alves Juliana de Ávila Barreto , Cerqueira Telma Cristina Fontes , De Santana Filho Valter Joviniano
Published: April 2023

ICU patients are exposed to several factors that can lead to muscle structural and functional changes, and ultrasonography can identify them. Although several studies have analyzed the reliability of muscle ultrasonography assessment, a protocol with more muscle assessments becomes a challenge. The aim of this study was to analyze the inter and intra-examiner reliability of peripheral and respiratory muscle ultrasonography assessment in critically ill patients. The sample size was 10 individuals aged ≥ 18 years who were admitted to the ICU. Practical training of four health professionals from different backgrounds was performed. After training, each examiner acquired three images to assess the thickness and echogenicity of the muscle groups: biceps brachii, forearm flexor group, quadriceps femoris, tibialis anterior and diaphragm. For the reliability analysis, an intraclass correlation coefficient was performed. Six hundred US images were analyzed for muscle thickness and 150 for echogenicity. Excellent intra-examiner reliability for echogenicity (ICC: 0.867–0.973) and inter-examiner reliability for thickness were found in all muscle groups (ICC: 0.778–0.942). For muscle thickness intra-examiner reliability, excellent results were found (ICC: 0.798–0.988), with a “good” correlation in one diaphragm assessment (ICC: 0.718). Excellent inter- and intra-examiner reliability of the thickness assessment and intra-examiner echogenicity of all muscles analyzed were found.
GIS Analysis of Adequate Accessibility to Public Transportation in Metropolitan Areas
Authors: Alamri Sultan , Adhinugraha Kiki , Allheeib Nasser , Taniar David
Published: April 2023

The public transport system plays an important role in a city as it moves people from one place to another efficiently and economically. The public transport network must be organized in a way that will cover as many places and as much of the population as possible, and support the city’s growth. As one of Australia’s largest capital cities, Melbourne is growing and expanding its metropolitan area to reflect the growth in population and an increased number of activities. To date, little research has been conducted to determine the accessibility and adequacy of public transport taking into consideration the blank spot areas, the number of public transport options for each area, the population density within specific geographical areas, and other issues. In this study, a new measurement model is developed that examines public transport in residential areas and the extent to which it is adequate for the various local government areas (LGAs). An accessibility approach is adopted to evaluate the accessibility of different types of public transportation in residential areas in metropolitan Melbourne, Victoria, Australia. The results show that in most LGAs, the number of blank spots will decrease as the population density increases. This indicates that residents in lower-density areas will have less accessibility to public transportation. However, there is no indication that there is a greater level of services (such as more night-time and weekend public transportation services) in the high-density areas. This research is significant as it will point to and help to improve the areas with inadequate public transportation and other issues, taking into consideration their geographical locations and population density.
Investigation of the Changes in Concentrations of Vitamin D-Binding Protein and Lactoferin in Plasma and Peritoneal Fluid of Patients with Endometriosis
Authors: Lisowska-Myjak Barbara , Skarżyńska Ewa , Wróbel Monika , Mańka Grzegorz , Kiecka Mariusz , Lipa Michał , Warzecha Damian , Spaczyński Robert , Piekarski Piotr , Banaszewska Beata , Jakimiuk Artur , Issat Tadeusz , Rokita Wojciech , Młodawski Jakub , Szubert Maria , Sieroszewski Piotr , Raba Grzegorz , Szczupak Kamil , Kluz Tomasz , Kluza Marek , Wielgoś Mirosław , Laudański Piotr
Published: April 2023

An evaluation of the association between the concentrations of vitamin D-binding protein and lactoferrin in the plasma and peritoneal fluid may facilitate the elucidation of molecular mechanisms in endometriosis. Vitamin D-binding protein and lactoferrin concentrations were measured by ELISA in plasma and peritoneal fluid samples from 95 women with suspected endometriosis as classified by laparoscopy into groups with (n = 59) and without endometriosis (n = 36). There were no differences (p > 0.05) in the plasma and peritoneal fluid concentrations of vitamin D-binding protein and lactoferrin between women with and without endometriosis. In women with endometriosis, there was a significant correlation between plasma and peritoneal fluid vitamin D-binding protein concentrations (r = 0.821; p = 0.000), but there was no correlation between lactoferrin concentrations in those compartments (r = 0.049; p > 0.05). Furthermore, in endometriosis, lactoferrin was found to correlate poorly with vitamin D-binding protein (r= −0.236; p > 0.05) in plasma, while in the peritoneal fluid, the correlation between those proteins was significant (r = 0.399; p = 0.002). The characteristic properties of vitamin D-binding protein and lactoferrin and the associations between their plasma and peritoneal fluid concentrations found in women with endometriosis may provide a novel panel of markers to identify high-risk patients in need of further diagnostic measures.
Compartmental Cerebrospinal Fluid Events Occurring after Subarachnoid Hemorrhage: An “Heparin Oriented” Systematic Review
Authors: Tartara Fulvio , Montalbetti Andrea , Crobeddu Emanuela , Armocida Daniele , Tavazzi Eleonora , Cardia Andrea , Cenzato Marco , Boeris Davide , Garbossa Diego , Cofano Fabio
Published: April 2023

Subarachnoid hemorrhage (SAH) represents a severe acute event with high morbidity and mortality due to the development of early brain injury (EBI), secondary delayed cerebral ischemia (DCI), and shunt-related hydrocephalus. Secondary events (SSE) such as neuroinflammation, vasospasm, excitotoxicity, blood-brain barrier disruption, oxidative cascade, and neuronal apoptosis are related to DCI. Despite improvement in management strategies and therapeutic protocols, surviving patients frequently present neurological deficits with neurocognitive impairment. The aim of this paper is to offer to clinicians a practical review of the actually documented pathophysiological events following subarachnoid hemorrhage. To reach our goal we performed a literature review analyzing reported studies regarding the mediators involved in the pathophysiological events following SAH occurring in the cerebrospinal fluid (CSF) (hemoglobin degradation products, platelets, complement, cytokines, chemokines, leucocytes, endothelin-1, NO-synthase, osteopontin, matricellular proteins, blood-brain barrier disruption, microglia polarization). The cascade of pathophysiological events secondary to SAH is very complex and involves several interconnected, but also distinct pathways. The identification of single therapeutical targets or specific pharmacological agents may be a limited strategy able to block only selective pathophysiological paths, but not the global evolution of SAH-related events. We report furthermore on the role of heparin in SAH management and discuss the rationale for use of intrathecal heparin as a pleiotropic therapeutical agent. The combination of the anticoagulant effect and the ability to interfere with SSE theoretically make heparin a very interesting molecule for SAH management.
Mesenchymal Stem Cell-Derived Extracellular Vesicles: Hype or Hope for Skeletal Muscle Anti-Frailty
Authors: Mahindran Elancheleyen , Wan Kamarul Zaman Wan Safwani , Ahmad Amin Noordin Khairul Bariah , Tan Yuen-Fen , Nordin Fazlina
Published: April 2023

Steadily rising population ageing is a global demographic trend due to the advancement of new treatments and technologies in the medical field. This trend also indicates an increasing prevalence of age-associated diseases, such as loss of muscle mass (sarcopenia), which tends to afflict the older population. The deterioration in muscle function can cause severe disability and seriously affects a patient’s quality of life. Currently, there is no treatment to prevent and reverse age-related skeletal muscle ageing frailty. Existing interventions mainly slow down and control the signs and symptoms. Mesenchymal stem cell-derived extracellular vesicle (MSC-EV) therapy is a promising approach to attenuate age-related skeletal muscle ageing frailty. However, more studies, especially large-scale randomised clinical trials need to be done in order to determine the adequacy of MSC-EV therapy in treating age-related skeletal muscle ageing frailty. This review compiles the present knowledge of the causes and changes regarding skeletal muscle ageing frailty and the potential of MSC-EV transplantation as a regenerative therapy for age-related skeletal muscle ageing frailty and its clinical trials.
Decidual Stromal Cell Ferroptosis Associated with Abnormal Iron Metabolism Is Implicated in the Pathogenesis of Recurrent Pregnancy Loss
Authors: Sun Fengrun , Cui Liyuan , Qian Jinfeng , Li Mengdie , Chen Lanting , Chen Chunqin , Li Dajin , Wang Songcun , Du Meirong
Published: April 2023

Iron is necessary for various critical biological processes, but iron overload is also dangerous since labile iron is redox-active and toxic. We found that low serum iron and decidual local iron deposition existed simultaneously in recurrent pregnancy loss (RPL) patients. Mice fed with a low-iron diet (LID) also showed iron deposition in the decidua and adverse pregnancy outcomes. Decreased ferroportin (cellular iron exporter) expression that inhibited the iron export from decidual stromal cells (DSCs) might be the reason for local iron deposition in DSCs from low-serum-iron RPL patients and LID-fed mice. Iron supplementation reduced iron deposition in the decidua of spontaneous abortion models and improved pregnancy outcomes. Local iron overload caused ferroptosis of DSCs by downregulating glutathione (GSH) and glutathione peroxidase 4 levels. Both GSH and cystine (for the synthesis of GSH) supplementation reduced iron-induced lipid reactive oxygen species (ROS) and cell death in DSCs. Ferroptosis inhibitor, cysteine, and GSH supplementation all effectively attenuated DSC ferroptosis and reversed embryo loss in the spontaneous abortion model and LPS-induced abortion model, making ferroptosis mitigation a potential therapeutic target for RPL patients. Further study that improves our understanding of low-serum-iron-induced DSC ferroptosis is needed to inform further clinical evaluations of the safety and efficacy of iron supplementation in women during pregnancy.
The Role of NQO1 in Ovarian Cancer
Authors: Tossetta Giovanni , Fantone Sonia , Goteri Gaia , Giannubilo Stefano Raffaele , Ciavattini Andrea , Marzioni Daniela
Published: April 2023

Ovarian cancer is one of the most dangerous gynecologic malignancies showing a high fatality rate because of late diagnosis and relapse occurrence due to chemoresistance onset. Several researchers reported that oxidative stress plays a key role in ovarian cancer occurrence, growth and development. The NAD(P)H:quinone oxidoreductase 1 (NQO1) is an antioxidant enzyme that, using NADH or NADPH as substrates to reduce quinones to hydroquinones, avoids the formation of the highly reactive semiquinones, then protecting cells against oxidative stress. In this review, we report evidence from the literature describing the effect of NQO1 on ovarian cancer onset and progression.
Identification of Novel Candidate Genes for Familial Thyroid Cancer by Whole Exome Sequencing
Authors: Tous Cristina , Muñoz-Redondo Carmen , Bravo-Gil Nereida , Gavilan Angela , Fernández Raquel María , Antiñolo Juan , Navarro-González Elena , Antiñolo Guillermo , Borrego Salud
Published: April 2023

Thyroid carcinoma (TC) can be classified as medullary (MTC) and non-medullary (NMTC). While most TCs are sporadic, familial forms of MTC and NMTC also exist (less than 1% and 3–9% of all TC cases, respectively). Germline mutations in RET are found in more than 95% of familial MTC, whereas familial NMTC shows a high degree of genetic heterogeneity. Herein, we aimed to identify susceptibility genes for familial NMTC and non-RET MTC by whole exome sequencing in 58 individuals belonging to 18 Spanish families with these carcinomas. After data analysis, 53 rare candidate segregating variants were identified in 12 of the families, 7 of them located in previously TC-associated genes. Although no common mutated genes were detected, biological processes regulating functions such as cell proliferation, differentiation, survival and adhesion were enriched. The reported functions of the identified genes together with pathogenicity and structural predictions, reinforced the candidacy of 36 of them, suggesting new loci related to TC and novel genotype–phenotype correlations. Therefore, our strategy provides clues to possible molecular mechanisms underlying familial forms of MTC and NMTC. These new molecular findings and clinical data of patients may be helpful for the early detection, development of tailored therapies and optimizing patient management.
AI-Predicted mTOR Inhibitor Reduces Cancer Cell Proliferation and Extends the Lifespan of C. elegans
Authors: Vidovic Tinka , Dakhovnik Alexander , Hrabovskyi Oleksii , MacArthur Michael R. , Ewald Collin Y.
Published: April 2023

The mechanistic target of rapamycin (mTOR) kinase is one of the top drug targets for promoting health and lifespan extension. Besides rapamycin, only a few other mTOR inhibitors have been developed and shown to be capable of slowing aging. We used machine learning to predict novel small molecules targeting mTOR. We selected one small molecule, TKA001, based on in silico predictions of a high on-target probability, low toxicity, favorable physicochemical properties, and preferable ADMET profile. We modeled TKA001 binding in silico by molecular docking and molecular dynamics. TKA001 potently inhibits both TOR complex 1 and 2 signaling in vitro. Furthermore, TKA001 inhibits human cancer cell proliferation in vitro and extends the lifespan of Caenorhabditis elegans, suggesting that TKA001 is able to slow aging in vivo.
Characterization of Seminal Microbiome of Infertile Idiopathic Patients Using Third-Generation Sequencing Platform
Authors: Garcia-Segura Sergio , del Rey Javier , Closa Laia , Garcia-Martínez Iris , Hobeich Carlos , Castel Ana Belén , Vidal Francisco , Benet Jordi , Oliver-Bonet Maria
Published: April 2023

Since the first description of a commensal seminal microbiome using sequencing, less than a decade ago, interest in the composition of this microbiome and its relationship with fertility has been growing. Articles using next-generation sequencing techniques agree on the identification of the most abundant bacterial phyla. However, at the genus level, there is still no consensus on which bacteria are most abundant in human seminal plasma. This discrepancy may be due to methodological variability such as sample collection, bacterial DNA extraction methodology, which hypervariable regions of 16S rRNA gene have been amplified, or bioinformatic analysis. In the present work, seminal microbiota of 14 control samples and 42 samples of idiopathic infertile patients were characterized based on full-length sequencing of the 16S rRNA gene using MinION platform from Oxford Nanopore. These same samples had been analyzed previously using Illumina’s MiSeq sequencing platform. Comparison between the results obtained with the two platforms has been used to analyze the impact of sequencing method on the study of the seminal microbiome’s composition. Seminal microbiota observed with MinION were mainly composed of the phyla Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria, with the most abundant genera being Peptoniphilus, Finegoldia, Staphylococcus, Anaerococcus, Campylobacter, Prevotella, Streptococcus, Lactobacillus, Ezakiella and Enterococcus. This composition was similar to that found by the Illumina platform, since these 10 most abundant genera were also among the most abundant genera detected by the Nanopore platform. In both cases, the top 10 genera represented more than 70% of the classified reads. However, relative abundance of each bacterium did not correlate between these two platforms, with intraindividual variations of up to 50 percentage points in some cases. Results suggest that the effect of the sequencing platform on the characterization of seminal microbiota is not very large at the phylum level, with slightly variances in Firmicutes and Actinobacteria, but presents differences at the genus level. These differences could alter the composition and diversity of bacterial profiles or posterior analyses. This indicates the importance of conducting multi-platform studies to better characterize seminal microbioma.
Activation of the Mechanosensitive Ion Channels Piezo1 and TRPV4 in Primary Human Healthy and Osteoarthritic Chondrocytes Exhibits Ion Channel Crosstalk and Modulates Gene Expression
Authors: Steinecker-Frohnwieser Bibiane , Lohberger Birgit , Toegel Stefan , Windhager Reinhard , Glanz Veronika , Kratschmann Cornelia , Leithner Andreas , Weigl Lukas
Published: April 2023

Osteoarthritis (OA) is the most common degenerative joint disease causing pain and functional limitations. Physical activity as a clinically relevant, effective intervention alleviates pain and promotes joint function. In chondrocytes, perception and transmission of mechanical signals are controlled by mechanosensitive ion channels, whose dysfunction in OA chondrocytes is leading to disease progression. Signaling of mechanosensitive ion channels Piezo/TRPV4 was analyzed by Yoda1/GSK1016790A application and calcium-imaging of Fura-2-loaded chondrocytes. Expression analysis was determined by qPCR and immunofluorescence in healthy vs. OA chondrocytes. Chondrocytes were mechanically stimulated using the Flexcell™ technique. Yoda1 and GSK1016790A caused an increase in intracellular calcium [Ca2+]i for Yoda1, depending on extracellularly available Ca2+. When used concomitantly, the agonist applied first inhibited the effect of subsequent agonist application, indicating mutual interference between Piezo/TRPV4. Yoda1 increased the expression of metalloproteinases, bone-morphogenic protein, and interleukins in healthy and OA chondrocytes to a different extent. Flexcell™-induced changes in the expression of MMPs and ILs differed from changes induced by Yoda1. We conclude that Piezo1/TRPV4 communicate with each other, an interference that may be impaired in OA chondrocytes. It is important to consider that mechanical stimulation may have different effects on OA depending on its intensity.
Relative Susceptibility of Brassicas to Cabbage Maggot (Diptera: Anthomyiidae) Infestation
Authors: Joseph Shimat V.
Published: April 2023

Cabbage maggot, Delia radicum (L.) (Diptera: Anthomyiidae) is a serious pest of Brassica such as broccoli (Brassica oleracea var. italica Plenck) and cauliflower (B. oleracea L. var. botrytis) in California’s Central Coast. Since there are limited non-chemical options available for growers to manage D. radicum, there is an urgent need to develop alternative tactics. The objective of this study was to determine the effects of side-by-side plantings of turnip (Brassica rapa var. rapa L.), lettuce (Lactuca sativa L.), cauliflower, and cabbage (B. oleracea L. var. capitata) with broccoli on D. radicum infestation. In 2013 and 2014, the experiments were conducted in Salinas, California. Significantly greater numbers of eggs and larval feeding damage were found on turnip compared with broccoli. Lettuce (Asteraceae), a non-Brassica crop, was compared with broccoli; however, lettuce did not reduce oviposition or larval feeding damage on broccoli. The larval feeding damage on cauliflower was significantly lower than on broccoli when planted side-by-side. The effects on cabbage were not significantly different from broccoli in terms of oviposition and larval feeding damage. This new information generated from the Central Coast of California will be further utilized to develop a trap crop to effectively tackle the D. radicum problem in Brassica fields.
Preservation of the Subtalar Joint Determines Outcomes in a 10-Year Evaluation of Ankle Arthrodesis
Authors: Sell Rebecca , Meinert Magalie , Herrmann Eva , Gramlich Yves , Klug Alexander , Neun Oliver , Hoffmann Reinhard , Fischer Sebastian
Published: April 2023

Posttraumatic osteoarthritis may lead to surgical fusion of the ankle joint if non-surgical therapy fails. The indication for a fusion of the joint is based on the pain and disability of the patient, radiographic imaging, and surgeon experience, with no strict guidelines. We aimed to compare outcomes after tibiotalocalcaneal arthrodesis (TTCA) and tibiotalar arthrodesis (TTA) to highlight the functional importance of the subtalar joint. In total, 432 patients with ankle arthrodesis were retrospectively enrolled. Group A (n = 216) underwent TTCA; group B (n = 216) underwent TTA. Demographics, Olerud & Molander Ankle Score (OMAS), Foot Function Index (FFI-D), and Short Form-12 Questionnaire (SF-12) were recorded at a mean follow-up of 6.2 years. The mean OMAS was 50.7; the mean FFI-D was 68.9; the mean SF-12 physical component summary was 39.1. These scores differed significantly between the groups (p < 0.001). The overall revision rate was 18%, primarily for revision of non-union and infection (p < 0.001). Approximately 16% of group A and 26% of group B were able to return to previous work (p < 0.001). Based on significantly worse clinical scores of TTCA compared to TTA and the prolonged downtime and permanent incapacity, the indication for a generous subtalar joint arthrodesis with planned ankle arthrodesis should always be critically examined.
The Use of Cognitive Tests in the Assessment of Dyslexia
Authors: Mather Nancy , Schneider Deborah
Published: April 2023

In this literature review, we address the use of cognitive tests, including intelligence tests, in the assessment and diagnosis of dyslexia, from both historic and present-day perspectives. We discuss the role of cognitive tests in the operationalization of the concepts of specificity and unexpectedness, two constructs considered essential to the characterization of dyslexia since the publication of early case reports in the late nineteenth century. We review the advantages and disadvantages of several approaches to specific learning disabilities’ identification that are used in schools. We also discuss contemporary debates around the use of standardized cognitive testing in dyslexia evaluations, in particular, the arguments of those who favor an approach to diagnosis based on prior history and the results of a comprehensive evaluation and those who favor an approach based on an individual’s response to intervention. We attempt to explain both perspectives by examining clinical observations and research findings. We then provide an argument for how cognitive tests can contribute to an accurate and informed diagnosis of dyslexia.
Sustainable Operations in Maritime Industry
Authors: Du Yuquan , Chen Gang , Wang Shuaian
Published: April 2023

Maritime transportation serves as the backbone of international trade and the global economy [...]
Deciphering Plant-Induced Responses toward Botrytis cinerea and Plasmopara viticola Attacks in Two Grapevine Cultivars Colonized by the Root Biocontrol Oomycete, Pythium oligandrum
Authors: Yacoub Amira , Haidar Rana , Mesguida Ouiza , Gerbore Jonathan , Hachicha Maya , Attard Eléonore , Guyoneaud Rémy , Rey Patrice
Published: April 2023

Two major diseases that affect grapevine leaves and berries are controlled by the oomycete Pythium oligandrum. As the efficacy of biocontrol agents strongly depends on factors such as the trophic behaviors of pathogens and cultivar susceptibility, a two-disease approach was implemented to evaluate the activity of P. oligandrum against Botrytis cinerea (the necrotrophic fungus of gray mold) and Plasmopara viticola (the biotrophic oomycete of downy mildew) on two grapevine cultivars with different susceptibilities to these two pathogens. The results show that grapevine root inoculation with P. oligandrum significantly reduced P. viticola and B. cinerea infection on the leaves of the two cultivars, but with differences. This was observed when the relative expression of 10 genes was measured in response to each pathogen, and could be attributed to their lifestyles, i.e., biotrophic or necrotrophic, which are related to the activation of specific metabolic pathways of the plant. In response to P. viticola infection, genes from the jasmonate and ethylene pathways were mainly induced, whereas for B. cinerea, the genes induced were those of the ethylene–jasmonate pathway. The different levels of defense against B. cinerea and P. viticola could also explain the difference in cultivar susceptibility to these pathogens.
Genetic Variability of HUPRA Syndrome—A Case Report
Authors: Petrosyan Edita , Molchanova Maria , Kushnir Berta , Povilaitite Patritsia , Tsygankova Polina , Zakharova Ekaterina , Proskura Maria
Published: April 2023

HUPRA syndrome is a rare autosomal recessive mitochondrial disorder caused by a mutation in the SARS2 gene encoding mitochondrial seryl-tRNA synthetase (mtSerRS). It includes hyperuricemia, pulmonary hypertension, renal failure, and alkalosis. We present a case report of a boy aged 1 year 2 months with premature anemia, hyperuricemia, pulmonary hypertension, renal failure, and alkalosis and diagnosed with HUPRA syndrome. This disease is known to be progressive and fatal. A genetic test revealed a new previously undescribed heterozygous nucleotide variant in exons 14 and 1 of the SARS2 gene. The nucleotide substitution c.1295G > A (p.Arg432His) was detected in exon 14; according to the criteria of the American College of Medical Genetics (ACMG), this missense mutation is probably pathogenic. The nucleotide substitution c.227T > C (p.Leu76Pro) was detected in exon 1; according to the ACMG criteria, this missense mutation is a variant of unclear significance. We suggest that previously undescribed nucleotide substitutions in the SARS2 gene revealed in a patient with typical clinical presentation of the HUPRA syndrome should be considered as a pathogenic mutation.
Impact of Drought on Land Productivity and Degradation in the Brazilian Semiarid Region
Authors: Paredes-Trejo Franklin , Barbosa Humberto Alves , Daldegan Gabriel Antunes , Teich Ingrid , García César Luis , Kumar T. V. Lakshmi , Buriti Catarina de Oliveira
Published: April 2023

The Brazilian semiarid region (BSR) has faced severe drought over the last three decades, which has led to a significant decline in land productivity, posing a considerable threat to food security and the local economy and communities. The United Nations Convention to Combat Desertification (UNCCD) has proposed the use of Earth observation-derived vegetation indices for monitoring land degradation across regions. In this study, we aim to evaluate three comprehensive UNCCD-recommended land productivity dynamic (LPD) approaches in the BSR by utilizing the standardized precipitation–evapotranspiration index (SPEI) at 12-month time scales as a benchmark drought index obtained from ground-based measurements. Our findings indicate that the LPD methods utilizing residual trends analysis (RESTREND), Trends.Earth (TE), and the Food and Agricultural Organization’s World Overview of Conservation Approaches and Technologies (FAO-WOCAT) are best suited for identifying degraded land areas in the BSR region compared to other approaches. However, it is advisable to use these methods with caution, since they do not fully capture the impact of drought on vegetation and may result in underestimating the extent of degraded areas. The RESTREND-based LPD, TE, and FAO-WOCAT estimate that the BSR region reached 213,248 km2, 248,075 km2, and 246,783 km2 of degraded land, respectively, between 2001 and 2015. These findings may be valuable for decision-makers involved in land management and conservation efforts in the Sertão region of Brazil.
A Comparative Analysis for Defining the Sliding Surface and Internal Structure in an Active Landslide Using the HVSR Passive Geophysical Technique in Pujilí (Cotopaxi), Ecuador
Authors: Alonso-Pandavenes Olegario , Bernal Daniela , Torrijo Francisco Javier , Garzón-Roca Julio
Published: April 2023

Geophysical techniques were employed to analyze one of the landslides that affected the main access road to Pujilí (Ecuador). A passive seismic technique was utilized to test a total of 70 horizontal to vertical spectral ratio (HVSR) points, complemented by an active seismic-refraction profile and a multichannel analysis of surface waves (MASW) survey. The results from the active geophysical surveys facilitated the determination of the shear-wave-velocity value for the surface materials that were in motion. However, the HVSR provided the fundamental frequency fo and amplification Ao values of the ground. The Nakamura (1989) relationship was applied to obtain the thickness of the sediments over a compact material from the fundamental frequency of the terrain in a two-layer model. Additionally, constrained models of the shear-wave velocity (Vs) distribution in the landslide area, obtained from the active seismic surveys, were used to invert the ellipticity curves. The results from this inversion were compared with those obtained by applying the Nakamura equation. The landslide-rupture surface was delineated for each type of analysis, which verified the correlation and minimal differences between the results of the three proposed studies, thus validating the procedure. The directivity of the microtremor HVSR signals was also analyzed, demonstrating a relationship with the internal structure of the sliding material. Furthermore, the ability to slide concerning the Kg parameter (vulnerability index, Nakamura, 1989) was studied. The usefulness of the directivity analysis in defining the internal structures in landslide materials and in determining the areas with the most significant instability was demonstrated. Overall, the HVSR is considered valuable when conducting early landslide studies and is helpful in determining the rupture plane while remaining a simple, fast, and economical technique.
Correction: Ralli and Rouvalis (2022). Μorphological Integration of Loan Words in Kaliardá. Languages 7: 167
Authors: Ralli Angela , Rouvalis Andreas
Published: April 2023

There was an error in the original publication (Ralli and Rouvalis 2022) [...]
The Effect of Mold Structure and Cooling Parameters on Heat Transfer during Billet High-Speed Continuous Casting
Authors: Wang Sijie , Xu Pei , Zhou Yongzhi , Duan Huamei , Chen Dengfu , Long Mujun
Published: April 2023

Mold structure and cooling parameters are significant factors that affect the heat transfer capacity of high-speed continuous casting molds of billets. Therefore, a three-dimensional fluid flow and heat transfer model of a 160 mm × 160 mm billet mold was established, and its accuracy was verified. Thereby, the characteristics of heat transfer and influences of mold structure and cooling parameters on heat transfer in the high-speed continuous casting billet mold region were revealed. It was found that extending the effective length of a mold is the most valuable method to improve its heat transfer capability and achieve high-speed continuous casting. The total heat and the shell thickness at a mold outlet increased by 19% and 9.21% on average with every 100 mm extension. Enlarging the fillet radius could enhance the uniformity of heat transfer in the mold. Considering the loss of material, the optimal fillet radius of the mold was determined to be R = 10 mm.
Influence of Strengthening Elements and Heat Treatment on Microstructure and Fracture Toughness of NiAl-Cr(Mo)-Based Eutectic Alloy
Authors: Wang Qiaoli , Li Rui , Xie Weixin , Yang Fang , Du Beining , Sheng Liyuan
Published: April 2023

Due to their potential improvement of high-temperature properties, the refractory metal hafnium (Hf) and the rare earth holmium (Ho) have attracted much attention. In the present research, NiAl-Cr(Mo) eutectic alloys with different Ho and Hf additions were fabricated by conventional smelting method and heat-treated to study the synergetic influence of strengthening elements and heat treatment. The samples were characterized using XRD, SEM, and TEM, and the three-point bending test was performed to obtain fracture toughness. The results demonstrate that Hf addition leads to the formation of Ni2AlHf Heusler phase and that Ho promoted the formation of Ni2Al3Ho phase. The microstructure of the alloy is affected by thermal treatment, with the coarsening of eutectic lamellae after heat treatment. The mechanical properties are improved by Hf and Ho additions, with increased fracture toughness. Overall, this study provides insights into the microstructure and properties of NiAl-Cr(Mo) eutectic alloys and highlights the potential of Hf and Ho addition to improve room-temperature properties. Specifically, the as-cast NiAl-Cr(Mo)-Hf eutectic alloy contains a relatively fine NiAl/Cr(Mo) eutectic lamella but coarse eutectic cell with Ni2AlHf phase embellished along the cell boundary. Minor Ho addition induces the formation of Ni2Al3Ho phase, which leads to the coarsening of the intercellular region but contributes to the refining of eutectic cell. In addition, the synergetic effect of Ho and Hf promotes the precipitation of Ni2Al3Ho and Ni2AlHf phases in the intercellular zone and increases the interface dislocations. Heat treatment benefits the solid solution of Ni2Al3Ho and Ni2AlHf phases, which improves their size and distribution by secondary precipitation. The Ni2AlHf phase in the NiAl-Cr(Mo)-Hf eutectic alloy becomes fine and uniformly distributed, but the NiAl/Cr(Mo) eutectic lamella in the eutectic cell becomes coarse. In comparison, heat treatment mainly optimizes the size and distribution of the Ni2Al3Ho and Ni2AlHf phases in the NiAl-Cr(Mo)-Hf-Ho eutectic alloy. Furthermore, heat treatment helps to eliminate the interface dislocations in the large NiAl precipitates and the NiAl/Cr(Mo) phase interfaces, which also contributes to fracture toughness by decreasing stress concentration. Minor Ho addition decreases the fracture toughness of as-cast NiAl-Cr(Mo)-Hf eutectic alloy from 6.7 to 6.1 MPa·m1/2, which should be ascribed to the coarsened intercellular region including aggregated Ni2Al3Ho and Ni2AlHf phases. However, minor Ho-doped NiAl-Cr(Mo)-Hf eutectic alloy obtained the highest fracture toughness of 8.2 MPa·m1/2 after heat treatment. This improved fracture toughness should be mainly attributed to the refined and well-distributed Ni2Al3Ho and Ni2AlHf phases in the heat-treated NiAl-Cr(Mo)-Hf-Ho eutectic alloy.