bluuebunny/crossref_metadata_2025 huggingface dataset:
Demand for a Housing Offer Addressed to Senior Citizens in Poland
Authors: Przybyła Katarzyna , Hełdak Maria , Kurtyka-Marcak Izabela
Published: November 2019

The purpose of this study is to identify the willingness of pensioners to move from an apartment/house to an apartment/house located in a housing estate specifically designed for senior citizens. As such, this article forms part of the discussion of the housing needs of senior citizens, their preferences and willingness to change their place of residence. The research covers a group of post-working-age people residing in the region of Lower Silesia in south-western Poland. Various research methods were used in the study, including a written questionnaire and its statistical analysis. The research focuses on analysing both the preferences of potential buyers of apartments for seniors and seniors’ willingness to change their place of residence in order to purchase an apartment adapted to their mobility needs (free from architectural and technical barriers), taking into account the respondent’s age and housing situation. The research indicates that city residents are significantly more often willing to change their current place of residence for one adapted to the needs of persons with disabilities than residents of rural areas. In turn, those living with their husband or wife are significantly more likely to state that special offers for seniors do not need to be developed than those living alone or with other family members or in a social care home. No relationship was found between the age of a respondent and the desire to move to a new house. However, younger seniors are more open to moving to housing estates offering facilities for the elderly who require additional care.
Diverged Preferences towards Sustainable Development Goals? A Comparison between Academia and the Communication Industry
Authors: Yeh Shin-Cheng , Chiou Haw-Jeng , Wu Ai-Wei , Lee Ho-Ching , Wu Homer C.
Published: November 2019

To understand how the sustainable development goals (SDGs) are involved and cited in different fields, the current study aimed to explore the key SDGs and SDG-pairs from the viewpoints of academia and the media. The academic publications with SDG(s)-related keywords in the Scopus database and the entry videos of the “2018 SDG Lions” were collected and analyzed through content and network analysis. It was found that SDG 3 and SDG 10 shared the highest preferences in both industries, but apparent gaps happened to SDG 5. The tied frequencies of the possible SDG pairs were also examined, and SDG 3-10 was identified taking the lead in both industries. Network analysis using degree centrality as the vital parameter demonstrated that SDG 8 and SDG 5 has strong connections with several SDGs for the academia and the media, respectively. The SDG-2-6-7 combination or “water-energy-food” nexus was also found the most frequent combination of three SDGs in the academia. Overall, SDG 3 can be treated as a unifying theme when seeking to acquire evidence-based knowledge for integrated implementation of the SDGs. Important implications for policy-making of the SDGs were also discussed.
The Process of Constructing a Health Tourism Destination Index
Authors: Lee Chia-Wen , Li Ching
Published: November 2019

The purpose of the study is to identify a set of key indicators with weightings for health tourism destinations by using an advanced analytic hierarchy process (AHP) method, derived from the official, academic, and professional opinions of the experts. The AHP method allocated weightings to the evaluation criteria selected by the fifteen experts. After expert evaluations were conducted, the three dimensions and eleven sub-dimensions of the initial health tourism destination were obtained as follows: (1) special demands and indications—medical care, health promotion, and tourism and leisure; (2) natural environment—climate, air, water, and light; (3) leisure activities and general demands—sports, therapeutic activities, interactions with animals and plants, and diet. The results revealed that the dimensions of special demands and indications were given the most attention and that the sub-dimensions of sports promotion were the highest ranked by expert groups. The official and academic opinions suggested that health tourism destinations should focus on special demands and indications, while professionals tended to consider the natural environment as a primary concern. In particular, they considered that good air quality can help people release pressure, relax, activate lymphocytes, improve immune function, and enhance disease immunity. The health tourism destination index can contribute to the overall strategic planning process by identifying improvements in activities and enhancing competitiveness in health tourism management by using benchmarking to further improve tourists’ experience and satisfaction.
Removal of Tetracycline by Hydrous Ferric Oxide: Adsorption Kinetics, Isotherms, and Mechanism
Authors: Zang Ji , Wu Tiantian , Song Huihui , Zhou Nan , Fan Shisuo , Xie Zhengxin , Tang Jun
Published: November 2019

The removal of tetracycline (TC) from solution is an important environmental issue. Here we prepared an adsorbent hydrous ferric oxide (HFO) by adjusting a FeCl3·6H2O solution to neutral pH. HFO was characterized by a surface area analyzer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS), and was used to remove TC from solution. The influence of pH, solid-to-liquid ratio, ionic type, and strength on TC removal was investigated. Adsorption kinetics and isotherms were also determined. HFO after adsorption of TC was analyzed by FTIR and XPS to investigate the adsorption mechanism. The results showed that the adsorption of TC increased from 88.3% to 95% with increasing pH (3.0–7.0) and then decreased. K+ ions had little effect on TC adsorption by HFO. However, Ca2+ and Mg2+ reduced the adsorption of TC on HFO. When the concentrations of Ca2+ and Mg2+ were increased, the inhibitory effect was more obvious. Pseudo-second-order kinetics and the Langmuir model fitted the adsorption process well. The maximum adsorption capacity of TC on HFO reached 99.49 mg·g−1. The adsorption process was spontaneous, endothermic, and increasingly disordered. Combination analysis with FTIR and XPS showed that the mechanism between TC and HFO involved electrostatic interactions, hydrogen interactions, and complexation. Therefore, the environmental behavior of TC could be affected by HFO.
Global Lifetime and 12-Month Prevalence of Suicidal Behavior, Deliberate Self-Harm and Non-Suicidal Self-Injury in Children and Adolescents between 1989 and 2018: A Meta-Analysis
Authors: Lim Kim-San , Wong Celine H. , McIntyre Roger S. , Wang Jiayun , Zhang Zhisong , Tran Bach X. , Tan Wanqiu , Ho Cyrus S. , Ho Roger C.
Published: November 2019

Objective: This meta-analysis aimed to estimate the global lifetime and 12-month prevalence of suicidal behavior, deliberate self-harm and non-suicidal self-injury in children and adolescents. Methods: A systematic search for relevant articles published between 1989 to 2018 was performed in multiple electronic databases. The aggregate 12-month and lifetime prevalence of suicidal behavior, deliberate self-harm, and non-suicidal self-injury were calculated based on the random-effects model. Subgroup analyses were performed to compare the prevalence according to school attendance and geographical regions. Results: A total of 686,672 children and adolescents were included. The aggregate lifetime and 12-month prevalence of suicide attempts was 6% (95% CI: 4.7–7.7%) and 4.5% (95% CI: 3.4–5.9%) respectively. The aggregate lifetime and 12-month prevalence of suicidal plan was 9.9% (95% CI: 5.5–17%) and 7.5% (95% CI: 4.5–12.1%) respectively. The aggregate lifetime and 12-month prevalence of suicidal ideation was 18% (95% CI: 14.2–22.7%) and 14.2% (95% CI: 11.6–17.3%) respectively. The aggregate lifetime and 12-month prevalence of non-suicidal self-injury was 22.1% (95% CI: 16.9–28.4%) and 19.5% (95% CI: 13.3–27.6%) respectively. The aggregate lifetime and 12-month prevalence of deliberate self-harm was 13.7% (95% CI: 11.0–17.0%) and 14.2% (95% CI: 10.1–19.5%) respectively. Subgroup analyses showed that full-time school attendance, non-Western countries, low and middle-income countries, and geographical locations might contribute to the higher aggregate prevalence of suicidal behaviors, deliberate self-harm, and non-suicidal self-injury. Conclusions: This meta-analysis found that non-suicidal self-injury, suicidal ideation, and deliberate self-harm were the three most common suicidal and self-harm behaviors in children and adolescents.
Monitoring of Low-Level Wind Shear by Ground-based 3D Lidar for Increased Flight Safety, Protection of Human Lives and Health
Authors: Nechaj Pavol , Gaál Ladislav , Bartok Juraj , Vorobyeva Olga , Gera Martin , Kelemen Miroslav , Polishchuk Volodymyr
Published: November 2019

Low-level wind shear, i.e., sudden changes in wind speed and/or wind direction up to altitudes of 1600 ft (500 m) above-ground is a hazardous meteorological phenomenon in aviation. It may radically change the aerodynamic circumstances of the flight, particularly during landing and take-off and consequently, it may threaten human lives and the health of passengers, people at the airport and its surrounding areas. The Bratislava Airport, the site of this case study, is one of the few airports worldwide and the first in Central Europe that is equipped with a Doppler lidar system, a perspective remote sensing tool for detecting low-level wind shear. The main objective of this paper was to assess the weather events collected over a period of one year with the occurrences of low-level wind shear situations, such as vertical discontinuities in the wind field, frontal passages and gust fronts to increase the level of flight safety and protect human lives and health. The lidar data were processed by a computer algorithm with the main focus on potential wind shear alerts and microburst alerts, guided by the recommendations of the International Civil Aviation Organisation. In parallel, the selected weather events were analyzed by the nearby located meteorological radar to utilize the strengths of both approaches. Additionally, an evaluation of the lidar capability to scan dynamics of aerosol content above the airport is presented.
Trends in Opioid Misuse among Marijuana Users and Non-Users in the U.S. from 2007–2017
Authors: Azagba Sunday , Shan Lingpeng , Manzione Lauren , Qeadan Fares , Wolfson Mark
Published: November 2019

Prescription-opioid misus e continues to be a significant health concern in the United States. The relationship between marijuana use and prescription-opioid misuse is not clear from the extant literature. This study examined national trends in prescription-opioid misuse among marijuana users and non-users using the 2007–2017 National Survey on Drug Use and Health. Cochran–Armitage tests were used to assess the statistical significance of changes in the yearly prevalence of prescription-opioid misuse and marijuana use. Multivariable logistic regression was used to examine the association between prescription-opioid and marijuana use adjusting for sociodemographic characteristics. From 2007 to 2017, marijuana use increased, while prescription-opioid misuse declined. Larger declines in prescription-opioid misuse were found among marijuana users than non-users. Marijuana ever-use was significantly associated with prescription-opioid misuse. Specifically, marijuana ever-users had higher odds of prescription-opioid misuse (ever-misuse [OR: 3.04; 95% CI, 2.68–3.43]; past-year misuse [OR: 3.44; 95% CI, 3.00–3.94]; and past-month misuse [OR: 4.50; 95% CI, 3.35–6.05]) compared to marijuana never-users. Similar results were found for the association of past-year and past-month marijuana use with prescription-opioid misuse. This study provides data on trends and associations about opioid misuse among marijuana users and non-users in a changing social environment of drug use in the United States. Future research should consider whether there is a causal relationship between marijuana use and prescription opioid misuse.
Is There a Need to Integrate Human Thermal Models with Weather Forecasts to Predict Thermal Stress?
Authors: Petersson Jakob , Kuklane Kalev , Gao Chuansi
Published: November 2019

More and more people will experience thermal stress in the future as the global temperature is increasing at an alarming rate and the risk for extreme weather events is growing. The increased exposure to extreme weather events poses a challenge for societies around the world. This literature review investigates the feasibility of making advanced human thermal models in connection with meteorological data publicly available for more versatile practices and a wider population. By providing society and individuals with personalized heat and cold stress warnings, coping advice and educational purposes, the risks of thermal stress can effectively be reduced. One interesting approach is to use weather station data as input for the wet bulb globe temperature heat stress index, human heat balance models, and wind chill index to assess heat and cold stress. This review explores the advantages and challenges of this approach for the ongoing EU project ClimApp where more advanced models may provide society with warnings on an individual basis for different thermal environments such as tropical heat or polar cold. The biggest challenges identified are properly assessing mean radiant temperature, microclimate weather data availability, integration and continuity of different thermal models, and further model validation for vulnerable groups.
Simulating Uneven Urban Spatial Expansion under Various Land Protection Strategies: Case Study on Southern Jiangsu Urban Agglomeration
Authors: Li Pingxing , Cao Hui
Published: November 2019

Urban spatial expansion (USE) is an uneven process affected by both natural and human factors, and land use regulation policy is of significance. To indicate the potential effects of different policies at a regional scale and then improving them under the context of increasing emphasis on land protection, we take Southern Jiangsu Urban Agglomeration (SJUA) in eastern China as a case study. Based on USE simulation with a minimum cumulative resistance (MCR) model under four scenarios related with arable and ecological land protection, we analyze the spatial differentiation of newly added urban construction lands and examine the changes of urban system with fractal analysis. Results indicate the allocations of newly added urban construction land differs by scenarios as well as total expanding amounts, and larger cities tend to grow faster. The share of the four largest cities (Suzhou, Nanjing(S), Wuxi, and Changzhou) were mostly higher than 40%. Accordingly, the final area of all cities was linearly corrected with their extant sizes in 2010. However, the differentiated allocations of newly added urban lands related to both increasing expanding amounts and different scenarios caused differences in the said linear relationships and also influenced urban rank-size in different degrees. It is concluded that the MCR model is feasible for simulating regional scale urban expansion and land protection strategies do not induce dramatic changes to the basic structures of regional urban system, but they are slightly different as land protection strategies change. The spatial distribution of protected lands affect the differentiation of both the predicted expanding amount of different cities and the regional urban systems significantly. It is of importance to optimize the spatial distribution of protected lands to regulate regional scale USE patterns and also urban systems properly.
Information Gain in Event Space Reflects Chance and Necessity Components of an Event
Authors: Weber Georg F.
Published: November 2019

Information flow for occurrences in phase space can be assessed through the application of the Lyapunov characteristic exponent (multiplicative ergodic theorem), which is positive for non-linear systems that act as information sources and is negative for events that constitute information sinks. Attempts to unify the reversible descriptions of dynamics with the irreversible descriptions of thermodynamics have replaced phase space models with event space models. The introduction of operators for time and entropy in lieu of traditional trajectories has consequently limited—to eigenvectors and eigenvalues—the extent of knowable details about systems governed by such depictions. In this setting, a modified Lyapunov characteristic exponent for vector spaces can be used as a descriptor for the evolution of information, which is reflective of the associated extent of undetermined features. This novel application of the multiplicative ergodic theorem leads directly to the formulation of a dimension that is a measure for the information gain attributable to the occurrence. Thus, it provides a readout for the magnitudes of chance and necessity that contribute to an event. Related algorithms express a unification of information content, degree of randomness, and complexity (fractal dimension) in event space.
Rolling-Bearing Fault-Diagnosis Method Based on Multimeasurement Hybrid-Feature Evaluation
Authors: Ge Jianghua , Yin Guibin , Wang Yaping , Xu Di , Wei Fen
Published: November 2019

To improve the accuracy of rolling-bearing fault diagnosis and solve the problem of incomplete information about the feature-evaluation method of the single-measurement model, this paper combines the advantages of various measurement models and proposes a fault-diagnosis method based on multi-measurement hybrid-feature evaluation. In this study, an original feature set was first obtained through analyzing a collected vibration signal. The feature set included time- and frequency-domain features, and also, based on the empirical-mode decomposition (EMD)-obtained time-frequency domain, energy and Lempel–Ziv complexity features. Second, a feature-evaluation framework of multiplicative hybrid models was constructed based on correlation, distance, information, and other measures. The framework was used to rank features and obtain rank weights. Then the weights were multiplied by the features to obtain a new feature set. Finally, the fault-feature set was used as the input of the category-divergence fault-diagnosis model based on kernel principal component analysis (KPCA), and the fault-diagnosis model was based on a support vector machine (SVM). The clustering effect of different fault categories was more obvious and classification accuracy was improved.
Comparative Study of Seismic Design and Performance of OMRF Building Using Indian, British, and European Codes
Authors: Rajeev Anupoju , Meena Naveen Kumar , Pallav Kumar
Published: November 2019

In India, damage cause by some major earthquakes, such as India/Nepal 2015, Sikkim 2011, Kashmir 2005, Bhuj 2001, Latur 1993, and Uttarkashi 1991, have raised alarms to professionals. The probability of seismic risk is higher in more densely populated Indian cities, such as Bhuj, Kashmir, Sikkim, Uttarkashi, as they come under the highest seismicity zone in India. Therefore, our primary interest is to investigate the seismic performance evaluation of the buildings in these seismic prone areas. Significant research has been conducted on the seismic performance of existing buildings. However, investigations on the seismic performance of a building with different country codes for the same earthquake event has not been explored, which is crucial in providing a deeper knowledge of the seismic performance of buildings. This paper presents a comparative study of an Ordinary Moment Resistant Frame (OMRF) building designed using three major codes, Indian (IS: 456-2000, IS: 1893-2002), British (BS: 8110-1997) and European (EC-2, EC-8). Six typical building models considered with earthquake (WiEQ), and without earthquake (WoEQ), and their assessments were interpreted using non-linear static analysis for determining their seismic performance. Seismic performance is compared in terms of base shear coefficient (BSC) and drift ratio that shows WiEQ models, at the drift ratio of 1.5%, the BSC was as follows; 0.78, 0.88, and 0.96 for the models designed for British, Euro, and Indian codes, respectively. The results show that the building models, that have been designed for the Indian codal provisions for both cases, performed well as compared to the other country codes. Base shear and drift ratio are the vital parameters that vary considerably among the building models. This aspect of the Indian code makes it a safer design methodology with higher reserve strength and a reasonably good displacement capacity before reaching the Collapse Prevention (CP) performance level.
Pharmaceutical Aspects of Artificial Nutrition
Authors: Reber Emilie , Messerli Markus , Stanga Zeno , Mühlebach Stefan
Published: November 2019

Artificial nutrition, including enteral (EN) and parenteral (PN) nutrition, is indicated whenever adequate oral nutrition fails to sufficiently supply the necessary nutrients to the body. It is a convenient, efficacious, safe, and well-tolerated form of clinical nutrition in the hospital and home setting. EN is administered via nasogastric tube or ostomies while PN usually requires a central venous access for administration, straight into the blood stream. The infused nutrients can then be taken up directly by the different organs. PN is targeted as a single daily portion formulated as an oil-in-water emulsion providing the necessary substrates for the catabolic and anabolic metabolism including macro- and micronutrients and fluids. PN has a complex pharmaceutical composition—all-in-one admixture—and its compounding or ready-to-use preparation. The use of PN is more challenging and more expensive compare to the use of EN, commercially available as ready-to-use formulations. EN and concomitant medication is highly challenging. Upon incorrect handling and administration, PN is associated with potentially severe or even fatal complications, mostly relating to the central venous access (e.g., catheter-related sepsis) or to a metabolic intolerance (e.g., hyperglycemia, refeeding syndrome) because of inappropriate administration. A correct order of admixing, correct dosing, and administration of the artificial is crucial for safety and efficacy; clinical and biochemical monitoring of the patient and treatment regimen adaption are necessary. The high number of reactive solutes allow only limited stability of a ready-to-use PN admixture. The potential for numerous incompatibilities and interactions renders PN admixtures generally unsuitable as drug vehicle. Laboratory compatibility and stability testing and pharmaceutical expertise are a prerequisite to define the PN composition including nutrients or even drugs admixed to define the appropriate and individualized nutrition and medication regimen. The aim of this narrative review is to present the actual state-of-the-art to deliver best quality artificial nutrition with special regard on pharmaceutical aspects such as instabilities, incompatibilities, and concomitant co-medication.
Impact of Support (MCF, ZrO2, ZSM-5) on the Efficiency of Ni Catalyst in High-Temperature Conversion of Lignocellulosic Biomass to Hydrogen-Rich Gas
Authors: Grams Jacek , Ryczkowski Robert , Chałupka Karolina , Sobczak Izabela , Rzeźnicka Izabela , Przybysz Kamila
Published: November 2019

The main objective of this work was to evaluate an impact of a support on the efficiency of nickel catalysts in the high-temperature conversion of lignocellulosic biomass to hydrogen-rich gas. The most important parameters influencing catalytic performance of the catalysts were identified. The properties of three materials (ZSM-5, ZrO2, and MCF (mesostructured cellular foam)) used as a support differing in surface acidity, surface area, pore structure, ability to interact with an active phase, and resistance to coking, have been studied. The results revealed that Ni/MCF, characterized by large pore size and pore volume, low acidity, small NiO crystallites size, and moderate interaction with the active phase, is the most efficient among studied catalysts, while an application of Ni on ZSM-5 support with high-acidity was not beneficial. The results suggest that structure of the support, in particular larger pore size and a better contact between an active phase and reaction intermediates, play an important role in the formation of gaseous products during thermal decomposition of lignocellulosic feedstock. On the other hand, high acidity of the support did not increase the formation of large amounts of hydrogen-rich gaseous products.
Synthesis of Poly (Citric Acid-Co-Glycerol) and Its Application as an Inhibitor of CaCO3 Deposition
Authors: Zahlan Hala , Saeed Waseem Sharaf , Alrasheed Radwan , Alandes Naser M. , Aouak Taieb
Published: November 2019

This investigation determined a feasible route to prepare hyperbranched polyesters involving citric acid (CA) and glycerol (GLC) monomers (CA-co-GLC) using a thermal polycondensation method. The synthesized copolymer was characterized using Fourier transform infrared spectroscopy (FT-IR), carbon-13 nuclear magnetic resonance spectroscopy, and differential scanning calorimetry. The ability of CA-co-GLC to inhibit deposition of inorganic scales such as calcium carbonate was investigated under varying temperature and pH medium. The evaluation of inhibition efficiency (IE) was conducted using the static scale inhibition method. The mechanism of the inhibitor’s action was investigated via growth solution analysis, measurement conductivity, and analysis of CaCO3 using FT-IR and scanning electron microscopy. The results obtained showed that the CA-co-GLC had good IE at an elevated temperature reaching 75% at 100 °C, pH 7.5, and 10 ppm copolymer dose. Using the same dose, the IE reached 66% at 50 °C and pH 10. The CA-co-GLC did not chelate Ca2+ in water, but led to a change in polymorphism, making it brittle and able to slip easily from the surface. Its action principally prevented the adhesion of calcium carbonate onto the surface.
Corrosion of Carbon Steel in Artificial Geothermal Brine: Influence of Carbon Dioxide at 70 °C and 150 °C
Authors: Aristia Gabriela , Hoa Le Quynh , Bäßler Ralph
Published: November 2019

This study focuses on the corrosion mechanism of carbon steel exposed to an artificial geothermal brine influenced by carbon dioxide (CO2) gas. The tested brine simulates a geothermal source in Sibayak, Indonesia, containing 1500 mg/L of Cl−, 20 mg/L of SO42−, and 15 mg/L of HCO3− with pH 4. To reveal the temperature effect on the corrosion behavior of carbon steel, exposure and electrochemical tests were carried out at 70 °C and 150 °C. Surface analysis of corroded specimens showed localized corrosion at both temperatures, despite the formation of corrosion products on the surface. After 7 days at 150 °C, SEM images showed the formation of an adherent, dense, and crystalline FeCO3 layer. Whereas at 70 °C, the corrosion products consisted of chukanovite (Fe2(OH)2CO3) and siderite (FeCO3), which are less dense and less protective than that at 150 °C. Control experiments under Ar-environment were used to investigate the corrosive effect of CO2. Free corrosion potential (Ecorr) and electrochemical impedance spectroscopy (EIS) confirm that at both temperatures, the corrosive effect of CO2 was more significant compared to that measured in the Ar-containing solution. In terms of temperature effect, carbon steel remained active at 70 °C, while at 150 °C, it became passive due to the FeCO3 formation. These results suggest that carbon steel is more susceptible to corrosion at the near ground surface of a geothermal well, whereas at a deeper well with a higher temperature, there is a possible risk of scaling (FeCO3 layer). A longer exposure test at 150 °C with a stagnant solution for 28 days, however, showed the unstable FeCO3 layer and therefore a deeper localized corrosion compared to that of seven-day exposed specimens.
Soil Recycling Geopolymers Fabricated from High Power Ultrasound Treated Soil Slurry in the Presence of Ammonia
Authors: Kwedi-Nsah Louis-Marly , Watanabe Yuta , Kobayashi Takaomi
Published: November 2019

Soil slurry was recycled to prepare a geopolymer after treatment with high-power ultrasound (US) in the presence of NH3, HCl, and NaOH. Under 28 kHz US, 0.1 M NH3 additives effectively decarbonized the slurry, eliminating 72.2% of the carbon content from the original soil. The US-treated soils were used as raw materials for the geopolymer, as they contained Si and Al components in the range of 25–30 and 8–10 wt.%, respectively. The geopolymer was prepared with a Na2SiO4/NaOH aqueous solution at a ¼ weight ratio at 80 °C for 24 h. The resultant geopolymers from the NH3-treated soil showed the best compressive strength of 3 MPa after 1 day of curing, with a low carbon content, when NH3 was used as an additive as opposed to HCl and NaOH under 1200 US exposure.
Design of a Novel Membrane Draft Tube Jet Loop Reactor (MDJLR) and Treatment of Slaughterhouse Wastewater
Authors: Farizoğlu Burhanettin , Uzuner Süleyman
Published: November 2019

The most important obstacle to the widespread use of membrane bioreactors (MBRs) is membrane fouling. In this study, a high-efficiency compact MBR was developed. Therefore, the draft tube of the jet loop reactor (JLB) was planned for use as a membrane module. The high-velocity jet streams, which are present according to the nature of the JLBs, provide high crossflow (cut-off force) on the membrane surface. Thus, the produced membrane module is operated in submerged membrane mode. This enhanced JLB modification is named the membrane draft tube jet loop reactor (MDJLR). This new system has a KLa value of 139 h−1 (at E/V of 2.24 kW m−3). In the next stage, treatment of slaughterhouse wastewater with the MDJLR was carried out. Under the 5.5 kg COD m−3 d−1 loading rate, efficiencies over 97% were achieved. The system operated continuously for 50 days without membrane backwashing or cleaning. During this period, fluxes of 3 L m−2·h−1 were approximately obtained at operating conditions of 850 mg L−1 MLSS (mixed liquor suspended solids) concentration, 1 bar suction pressure (∆P), and 3000 L h−1 circulation rate. This developed MDJLR will make jet loop membrane bioreactors (JLMBRs) and MBRs more compact and improve their performance.
Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
Authors: Sano Emi , Mori Chihiro , Matsuoka Naoki , Ozaki Yuka , Yagi Keisuke , Wada Aya , Tashima Koichi , Yamasaki Shinsuke , Tanabe Kana , Yano Kayo , Torisawa Yu-suke
Published: November 2019

Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hydrophobic molecules are strongly absorbed. This drawback hinders widespread use of PDMS-based devices for drug discovery and development. Here, we describe a microfluidic cell culture system made of a tetrafluoroethylene-propylene (FEPM) elastomer. We demonstrated that FEPM does not absorb small hydrophobic compounds including rhodamine B and three types of drugs, nifedipine, coumarin, and Bay K8644, whereas PDMS absorbs them strongly. The device consists of two FEPM layers of microchannels separated by a thin collagen vitrigel membrane. Since FEPM is flexible and biocompatible, this microfluidic device can be used to culture cells while applying mechanical strain. When human umbilical vein endothelial cells (HUVECs) were subjected to cyclic strain (~10%) for 4 h in this device, HUVECs reoriented and aligned perpendicularly in response to the cyclic stretch. Moreover, we demonstrated that this device can be used to replicate the epithelial–endothelial interface as well as to provide physiological mechanical strain and fluid flow. This method offers a robust platform to produce organs-on-chips for drug discovery and development.
Phylogeny of Anoxygenic Photosynthesis Based on Sequences of Photosynthetic Reaction Center Proteins and a Key Enzyme in Bacteriochlorophyll Biosynthesis, the Chlorophyllide Reductase
Authors: Imhoff Johannes F. , Rahn Tanja , Künzel Sven , Neulinger Sven C.
Published: November 2019

Photosynthesis is a key process for the establishment and maintenance of life on earth, and it is manifested in several major lineages of the prokaryote tree of life. The evolution of photosynthesis in anoxygenic photosynthetic bacteria is of major interest as these have the most ancient roots of photosynthetic systems. The phylogenetic relations between anoxygenic phototrophic bacteria were compared on the basis of sequences of key proteins of the type-II photosynthetic reaction center, including PufLM and PufH (PuhA), and a key enzyme of bacteriochlorophyll biosynthesis, the light-independent chlorophyllide reductase BchXYZ. The latter was common to all anoxygenic phototrophic bacteria, including those with a type-I and those with a type-II photosynthetic reaction center. The phylogenetic considerations included cultured phototrophic bacteria from several phyla, including Proteobacteria (138 species), Chloroflexi (five species), Chlorobi (six species), as well as Heliobacterium modesticaldum (Firmicutes), Chloracidobacterium acidophilum (Acidobacteria), and Gemmatimonas phototrophica (Gemmatimonadetes). Whenever available, type strains were studied. Phylogenetic relationships based on a photosynthesis tree (PS tree, including sequences of PufHLM-BchXYZ) were compared with those of 16S rRNA gene sequences (RNS tree). Despite some significant differences, large parts were congruent between the 16S rRNA phylogeny and photosynthesis proteins. The phylogenetic relations demonstrated that bacteriochlorophyll biosynthesis had evolved in ancestors of phototrophic green bacteria much earlier as compared to phototrophic purple bacteria and that multiple events independently formed different lineages of aerobic phototrophic purple bacteria, many of which have very ancient roots. The Rhodobacterales clearly represented the youngest group, which was separated from other Proteobacteria by a large evolutionary gap.
Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate
Authors: Szotkowski Martin , Byrtusova Dana , Haronikova Andrea , Vysoka Marie , Rapta Marek , Shapaval Volha , Marova Ivana
Published: November 2019

Carotenogenic yeasts are non-conventional oleaginous microorganisms capable of utilizing various waste substrates. In this work, four red yeast strains (Rhodotorula, Cystofilobasidium, and Sporobolomyces sp.) were cultivated in media containing crude, emulsified, and enzymatically hydrolyzed animal waste fat, compared with glucose and glycerol, as single C-sources. Cell morphology (cryo-SEM (cryo-scanning electron microscopy), TEM (transmission electron microscopy)), production of biomass, lipase, biosurfactants, lipids (gas chromatography/flame ionization detection, GC/FID) carotenoids, ubiquinone, and ergosterol (high performance liquid chromatography, HPLC/PDA) in yeast cells was studied depending on the medium composition, the C source, and the carbon/nitrogen (C/N) ratio. All studied strains are able to utilize solid and processed fat. Biomass production at C/N = 13 was higher on emulsified/hydrolyzed fat than on glucose/glycerol. The production of lipids and lipidic metabolites was enhanced for several times on fat; the highest yields of carotenoids (24.8 mg/L) and lipids (54.5%/CDW (cell dry weight)) were found in S. pararoseus. Simultaneous induction of lipase and biosurfactants was observed on crude fat substrate. An increased C/N ratio (13–100) led to higher biomass production in fat media. The production of total lipids increased in all strains to C/N = 50. Oppositely, the production of carotenoids, ubiquinone, and ergosterol dramatically decreased with increased C/N in all strains. Compounds accumulated in stressed red yeasts have a great application potential and can be produced efficiently during the valorization of animal waste fat under the biorefinery concept.
Microbial Surfactants: The Next Generation Multifunctional Biomolecules for Applications in the Petroleum Industry and Its Associated Environmental Remediation
Authors: Fenibo Emmanuel O. , Ijoma Grace N. , Selvarajan Ramganesh , Chikere Chioma B.
Published: November 2019

Surfactants are a broad category of tensio-active biomolecules with multifunctional properties applications in diverse industrial sectors and processes. Surfactants are produced synthetically and biologically. The biologically derived surfactants (biosurfactants) are produced from microorganisms, with Pseudomonas aeruginosa, Bacillus subtilis Candida albicans, and Acinetobacter calcoaceticus as dominant species. Rhamnolipids, sophorolipids, mannosylerithritol lipids, surfactin, and emulsan are well known in terms of their biotechnological applications. Biosurfactants can compete with synthetic surfactants in terms of performance, with established advantages over synthetic ones, including eco-friendliness, biodegradability, low toxicity, and stability over a wide variability of environmental factors. However, at present, synthetic surfactants are a preferred option in different industrial applications because of their availability in commercial quantities, unlike biosurfactants. The usage of synthetic surfactants introduces new species of recalcitrant pollutants into the environment and leads to undesired results when a wrong selection of surfactants is made. Substituting synthetic surfactants with biosurfactants resolves these drawbacks, thus interest has been intensified in biosurfactant applications in a wide range of industries hitherto considered as experimental fields. This review, therefore, intends to offer an overview of diverse applications in which biosurfactants have been found to be useful, with emphases on petroleum biotechnology, environmental remediation, and the agriculture sector. The application of biosurfactants in these settings would lead to industrial growth and environmental sustainability.
Methane-Mediated Vapor Transport Growth of Monolayer WSe2 Crystals
Authors: Jang Hyeon-Sik , Lim Jae-Young , Kang Seog-Gyun , Hyun Sang-Hwa , Sandhu Sana , Son Seok-Kyun , Lee Jae-Hyun , Whang Dongmok
Published: November 2019

The electrical and optical properties of semiconducting transition metal dichalcogenides (TMDs) can be tuned by controlling their composition and the number of layers they have. Among various TMDs, the monolayer WSe2 has a direct bandgap of 1.65 eV and exhibits p-type or bipolar behavior, depending on the type of contact metal. Despite these promising properties, a lack of efficient large-area production methods for high-quality, uniform WSe2 hinders its practical device applications. Various methods have been investigated for the synthesis of large-area monolayer WSe2, but the difficulty of precisely controlling solid-state TMD precursors (WO3, MoO3, Se, and S powders) is a major obstacle to the synthesis of uniform TMD layers. In this work, we outline our success in growing large-area, high-quality, monolayered WSe2 by utilizing methane (CH4) gas with precisely controlled pressure as a promoter. When compared to the catalytic growth of monolayered WSe2 without a gas-phase promoter, the catalytic growth of the monolayered WSe2 with a CH4 promoter reduced the nucleation density to 1/1000 and increased the grain size of monolayer WSe2 up to 100 μm. The significant improvement in the optical properties of the resulting WSe2 indicates that CH4 is a suitable candidate as a promoter for the synthesis of TMD materials, because it allows accurate gas control.
Plasma versus Erythrocyte Vitamin E in Renal Transplant Recipients, and Duality of Tocopherol Species
Authors: Sotomayor Camilo G. , Rodrigo Ramón , Gomes-Neto António W. , Gormaz Juan Guillermo , Pol Robert A. , Minović Isidor , Eggersdorfer Manfred L. , Vos Michel , Riphagen Ineke J. , de Borst Martin H. , Nolte Ilja M. , Berger Stefan P. , Navis Gerjan J. , Bakker Stephan J. L.
Published: November 2019

Redox imbalance is an adverse on-going phenomenon in renal transplant recipients (RTR). Vitamin E has important antioxidant properties that counterbalance its deleterious effects. However, plasma vitamin E affinity with lipids challenges interpretation of its levels. To test the hypothesis that erythrocyte membranes represent a lipids-independent specimen to estimate vitamin E status, we performed a cross-sectional study in a cohort of adult RTR (n = 113) recruited in a university setting (2015–2018). We compared crude and total lipids-standardized linear regression-derived coefficients of plasma and erythrocyte tocopherol species in relation to clinical and laboratory parameters. Strongly positive associations of fasting lipids with plasma tocopherol became inverse, rather than absent, in total lipids-standardized analyses, indicating potential overadjustment. Whilst, no variables from the lipids domain were associated with the tocopherol species measured from erythrocyte specimens. In relation to inflammatory status and clinical parameters with antioxidant activity, we found associations in directions that are consistent with either beneficial or adverse effects concerning α- or γ-tocopherol, respectively. In conclusion, erythrocytes offer a lipids-independent alternative to estimate vitamin E status and investigate its relationship with parameters over other biological domains. In RTR, α- and γ-tocopherol may serve as biomarkers of relatively lower or higher vulnerability to oxidative stress and inflammation, noticeably in opposite directions.
Exercise Mitigates the Loss of Muscle Mass by Attenuating the Activation of Autophagy during Severe Energy Deficit
Authors: Martin-Rincon Marcos , Pérez-López Alberto , Morales-Alamo David , Perez-Suarez Ismael , de Pablos-Velasco Pedro , Perez-Valera Mario , Perez-Regalado Sergio , Martinez-Canton Miriam , Gelabert-Rebato Miriam , Juan-Habib Julian William , Holmberg Hans-Christer , Calbet Jose A L
Published: November 2019

The loss of skeletal muscle mass with energy deficit is thought to be due to protein breakdown by the autophagy-lysosome and the ubiquitin-proteasome systems. We studied the main signaling pathways through which exercise can attenuate the loss of muscle mass during severe energy deficit (5500 kcal/day). Overweight men followed four days of caloric restriction (3.2 kcal/kg body weight day) and prolonged exercise (45 min of one-arm cranking and 8 h walking/day), and three days of control diet and restricted exercise, with an intra-subject design including biopsies from muscles submitted to distinct exercise volumes. Gene expression and signaling data indicate that the main catabolic pathway activated during severe energy deficit in skeletal muscle is the autophagy-lysosome pathway, without apparent activation of the ubiquitin-proteasome pathway. Markers of autophagy induction and flux were reduced by exercise primarily in the muscle submitted to an exceptional exercise volume. Changes in signaling are associated with those in circulating cortisol, testosterone, cortisol/testosterone ratio, insulin, BCAA, and leucine. We conclude that exercise mitigates the loss of muscle mass by attenuating autophagy activation, blunting the phosphorylation of AMPK/ULK1/Beclin1, and leading to p62/SQSTM1 accumulation. This includes the possibility of inhibiting autophagy as a mechanism to counteract muscle loss in humans under severe energy deficit.
Impact of Vitamin D on Physical Efficiency and Exercise Performance—A Review
Authors: Wiciński Michał , Adamkiewicz Dawid , Adamkiewicz Monika , Śniegocki Maciej , Podhorecka Marta , Szychta Paweł , Malinowski Bartosz
Published: November 2019

Vitamin D deficiency amongst athletes and the general population seems to be a prominent problem. The most recognized role of vitamin D is its regulation of calcium homeostasis; there is a strong relationship between vitamin D and bone health. Moreover, its concentrations are associated with muscle function and immune response in both the general and athletic populations. Vitamin D level is strongly connected with the presence of VDRs (vitamin D receptors) in most human extraskeletal cells. Expression of multiple myogenic transcription factors enhancing muscle cell proliferation and differentiation is caused by an exposure of skeletal muscles to vitamin D. The aim of this review is to summarize current understanding of the significance of vitamin D on exercise performance and physical efficiency, as well to analyze the impact of vitamin D on multiple potential mechanisms. More high-quality research studies, considering free 25(OH)D as a better marker of vitamin D status, the baseline level of 25(OH)D and multiple pathways of vitamin D acting and usage in athletes are required.
Comparison of the Effects of Fasting Glucose, Hemoglobin A1c, and Triglyceride–Glucose Index on Cardiovascular Events in Type 2 Diabetes Mellitus
Authors: Su Wei-Yu , Chen Szu-Chia , Huang Yu-Ting , Huang Jiun-Chi , Wu Pei-Yu , Hsu Wei-Hao , Lee Mei-Yueh
Published: November 2019

The triglyceride–glucose (TyG) index has been correlated with insulin resistance. We aim to investigate the role of the TyG index on cardiovascular (CV) events in type 2 diabetes mellitus and compare the roles of fasting glucose, hemoglobin A1c, and the TyG index in predicting CV events. This retrospective study enrolled 3524 patients with type 2 diabetes from the Kaohsiung Medical University Research Database in 2009 in this longitudinal study and followed them until 2015. The TyG index was calculated as log (fasting triglyceride level (mg/dL) × fasting glucose level (mg/dL)/2). CV events included myocardial infarction, unstable angina, stroke, hospitalization for coronary artery disease, peripheral artery disease, and CV-related death. The association between variables and CV events was assessed using a multivariable stepwise Cox proportional hazard analysis. Two hundred and fifteen CV events (6.1%) were recorded during a follow-up period of 5.93 years. The multivariable stepwise analysis showed that high fasting glucose (HR, 1.007; p < 0.001) and a high TyG index (HR, 1.521; p = 0.004) but not hemoglobin A1c or triglycerides were associated with a higher rate of CV events. Adding fasting glucose and the TyG index to the basic model improved the predictive ability of progression to a CV event (p < 0.001 and p = 0.018, respectively), over that of hemoglobin A1c (p = 0.084) and triglyceride (p = 0.221). Fasting glucose and the TyG index are useful parameters and stronger predictive factors than hemoglobin A1c and triglyceride for CV events and may offer an additional prognostic benefit in patients with type 2 diabetes.