bluuebunny/crossref_metadata_2025 huggingface dataset:
An Investigation of the Variations in Complete Mitochondrial Genomes of Lingula anatina in the Western Pacific Region
Authors: Karagozlu Mustafa Zafer , Do Thinh Dinh , Kim Jung-Il , Choi Tae-June , Kim Seong-Geun , Kim Chang-Bae
Published: April 2021

Lingula anatina is a brachiopod widely distributed in the western Pacific region. Even though L. anatina has been targeted for a number of biological studies, there is still limited information on intraspecific genetic variations of L. anatina. In this study, L. anatina specimens were collected from Korea and Vietnam, and complete mitochondrial genome (mitogenome) sequences were analyzed and compared with previous records. The total mitogenomes of L. anatina were 24,875 bp and 25,305 bp in size for Korean and Vietnamese specimens, respectively. Those mitogenomes are extraordinarily longer than the typical mitogenome size for an animal but shorter than the previous record from Yanagawa (Japan) for this species. The gene orders and the sizes of the protein-coding genes are also different from those for the Japanese specimen. Furthermore, the nonsynonymous (Ka) and synonymous (Ks) substitution rates in protein-coding genes (PCGs) were calculated to test the idea of evolutionary rate differences in mitochondrial genomes. The analyses showed relatively low Ka and Ks for the complete mitogenomes from Buan (Korea), Doson (Vietnam) and Yanagawa (Japan). The Ka/Ks ratio was less than 1 in comparisons of three localities, indicating the existence of purifying selection in this species. The phylogenetic analyses showed that L. anatina diverged among localities in the western Pacific region.
The Relation between Physiological Parameters and Colour Modifications in Text Background and Overlay during Reading in Children with and without Dyslexia
Authors: Jakovljević Tamara , Janković Milica M. , Savić Andrej M. , Soldatović Ivan , Čolić Gordana , Jakulin Tadeja Jere , Papa Gregor , Ković Vanja
Published: April 2021

Reading is one of the essential processes during the maturation of an individual. It is estimated that 5–10% of school-age children are affected by dyslexia, the reading disorder characterised by difficulties in the accuracy or fluency of word recognition. There are many studies which have reported that coloured overlays and background could improve the reading process, especially in children with reading disorders. As dyslexia has neurobiological origins, the aim of the present research was to understand the relationship between physiological parameters and colour modifications in the text and background during reading in children with and without dyslexia. We have measured differences in electroencephalography (EEG), heart rate variability (HRV), electrodermal activities (EDA) and eye movements of the 36 school-age (from 8 to 12 years old) children (18 with dyslexia and 18 of control group) during the reading task in 13 combinations of background and overlay colours. Our findings showed that the dyslexic children have longer reading duration, fixation count, fixation duration average, fixation duration total, and longer saccade count, saccade duration total, and saccade duration average while reading on white and coloured background/overlay. It was found that the turquoise background, turquoise overlay, and yellow background colours are beneficial for dyslexic readers, as they achieved the shortest time duration of the reading tasks when these colours were used. Additionally, dyslexic children have higher values of beta (15–40 Hz) and the broadband EEG (0.5–40 Hz) power while reading in one particular colour (purple), as well as increasing theta range power while reading with the purple overlay. We have observed no significant differences between HRV parameters on white colour, except for single colours (purple, turquoise overlay, and yellow overlay) where the control group showed higher values for mean HR, while dyslexic children scored higher with mean RR. Regarding EDA measure, we found systematically lower values in children with dyslexia in comparison to the control group. Based on the present results, we can conclude that both pastel and intense background/overlays are beneficial for reading of both groups and all sensor modalities could be used to better understand the neurophysiological origins in dyslexic children.
The Effects of Internal Attention on Knee Biomechanics in Volleyball Spike Jump through Augmented Video Feedback
Authors: Wang Aiwen , Li Xiaohan , Huang Huiming
Published: April 2021

Poor knee biomechanics in a volleyball spike jump generally result in a higher knee injury risk, which can be altered by an internal focus of attention (FOA). The constrained action hypothesis (CAH) purports that the FOA inhibits sports performance whereas no ecologically valid evidence has been found in previous studies. The purpose of this research is to explore the effect of video feedback on knee biomechanics in a volleyball spike jump including landing and take-off phases. The video feedback was performed in a natural way. Fourteen volleyball male players were recruited in this study. A paired t-test was used to detect the effect of the feedback; meanwhile, statistical parameter mapping (SPM) statistics were used for the continuum differences during movement. After biofeedback, the initial contact flexion angle of the knee (t = 2.179, p = 0.049), the maximal flexion angle of the knee (t = 3.242, p = 0.006) and the maximal internal rotation angular velocity of the knee (t = 5.209, p = 0.003) increased significantly; the maximal extension moment of the knee (t = 3.962, p < 0.001) and the maximal flexion moment of the knee (t = −3.711, p = 0.002) significantly decreased; the maximal abduction moment significantly decreased (t = 3.069, p = 0.037) but the maximal internal rotation moment significantly increased (t = 2.813, p = 0.018); the first peak of the vertical ground reaction force (vGRF) (t = 7.618, p < 0.001) and the average loading rate to the first peak (t = 4.205, p = 0.004) significantly decreased; the other peaks of the vGRF were not found to have differences; a larger knee flexion was found during the phase from 31.17 to 73.19% (t = 2.611, p = 0.012); a larger adduction angular velocity was found during the phase from 49.07 to 62.46% (t = 3.148, p = 0.004); a smaller external rotational angular velocity was found during the phase from 45.85 to 49.96% (t = 5.011 p = 0.017); there was an increased flexion moment of the knee during the phase from 19.72 to 21.38% (t = 0.029, p = 0.029) and an external moment of the knee during the phase from 85.55 to 95.06% (t = 4.214, p < 0.001); the vGRF significantly decreased during the phase from 3.13 to 5.94% (t = 4.096, p = 0.014) and 19.83–21.97% (t = 4.096, p = 0.024) but significantly increased in the phase of 91.43–100% (t = 4.096, p < 0.001). The impulse of the vGRF and knee power were not found to be different compared with before biofeedback. Therefore, our study suggests video feedback in a natural practice has the potential to improve knee movement whilst not altering the performance in a volleyball spike jump. This indicates that the CAH theory is possibly not suitable in a real competition. Due to the complexity of human movements and the limitations of this study, muscle activities must be considered in the future.
Identification of TLR2 Signalling Mechanisms Which Contribute to Barrett’s and Oesophageal Adenocarcinoma Disease Progression
Authors: Flis Ewelina , Barber Gillian , Nulty Ciara , Keogh Brian , McGuirk Peter , Anand Akanksha , O’Sullivan Jacintha , Quante Michael , Creagh Emma M.
Published: April 2021

Chronic inflammation plays an important role in the pathogenesis of oesophageal adenocarcinoma (EAC) and its only known precursor, Barrett’s oesophagus (BE). Recent studies have shown that oesophageal TLR2 levels increase from normal epithelium towards EAC. TLR2 signalling is therefore likely to be important during EAC development and progression, which requires an inflammatory microenvironment. Here, we show that, in response to TLR2 stimulation, BE organoids and early-stage EAC cells secrete pro-inflammatory cytokines and chemokines which recruit macrophages to the tumour site. Factors secreted from TLR2-stimulated EAC cells are shown to subsequently activate TLR2 on naïve macrophages, priming them for inflammasome activation and inducing their differentiation to an M2/TAM-like phenotype. We identify the endogenous TLR2 ligand, HMGB1, as the factor secreted from EAC cells responsible for the observed TLR2-mediated effects on macrophages. Our results indicate that HMGB1 signalling between EAC cells and macrophages creates an inflammatory tumour microenvironment to facilitate EAC progression. In addition to identifying HMGB1 as a potential target for early-stage EAC treatment, our data suggest that blocking TLR2 signalling represents a mechanism to limit HMGB1 release, inflammatory cell infiltration and inflammation during EAC progression.
Characterization of Cell-Bound CA125 on Immune Cell Subtypes of Ovarian Cancer Patients Using a Novel Imaging Platform
Authors: González Germán , Lakatos Kornél , Hoballah Jawad , Fritz-Klaus Roberta , Al-Johani Lojain , Brooker Jeff , Jeong Sinyoung , Evans Conor L. , Krauledat Petra , Cramer Daniel W. , Hoffman Robert A. , Hansen W. Peter , Patankar Manish S.
Published: April 2021

MUC16, a sialomucin that contains the ovarian cancer biomarker CA125, binds at low abundance to leucocytes via the immune receptor, Siglec-9. Conventional fluorescence-based imaging techniques lack the sensitivity to assess this low-abundance event, prompting us to develop a novel “digital” optical cytometry technique for qualitative and quantitative assessment of CA125 binding to peripheral blood mononuclear cells (PBMC). Plasmonic nanoparticle labeled detection antibody allows assessment of CA125 at the near-single molecule level when bound to specific immune cell lineages that are simultaneously identified using multiparameter fluorescence imaging. Image analysis and deep learning were used to quantify CA125 per each cell lineage. PBMC from treatment naïve ovarian cancer patients (N = 14) showed higher cell surface abundance of CA125 on the aggregate PBMC population as well as on NK (p = 0.013), T (p < 0.001) and B cells (p = 0.024) compared to circulating lymphocytes of healthy donors (N = 7). Differences in CA125 binding to monocytes or NK-T cells between the two cohorts were not significant. There was no correlation between the PBMC-bound and serum levels of CA125, suggesting that these two compartments are not in stoichiometric equilibrium. Understanding where and how subset-specific cell-bound surface CA125 takes place may provide guidance towards a new diagnostic biomarker in ovarian cancer.
Discovering the Mutational Profile of Early Colorectal Lesions: A Translational Impact
Authors: Alquati Chiara , Prossomariti Anna , Piazzi Giulia , Buttitta Francesco , Bazzoli Franco , Laghi Luigi , Ricciardiello Luigi
Published: April 2021

Colorectal cancer (CRC) develops through a multi-step process characterized by the acquisition of multiple somatic mutations in oncogenes and tumor-suppressor genes, epigenetic alterations and genomic instability. These events lead to the progression from precancerous lesions to advanced carcinomas. This process requires several years in a sporadic setting, while occurring at an early age and or faster in patients affected by hereditary CRC-predisposing syndromes. Since advanced CRC is largely untreatable or unresponsive to standard or targeted therapies, the endoscopic treatment of colonic lesions remains the most efficient CRC-preventive strategy. In this review, we discuss recent studies that have assessed the genetic alterations in early colorectal lesions in both hereditary and sporadic settings. Establishing the genetic profile of early colorectal lesions is a critical goal in the development of risk-based preventive strategies.
Immunomodulatory Factors in Primary Endometrial Cell Cultures Isolated from Cancer and Noncancerous Human Tissue–Focus on RAGE and IDO1
Authors: Tkaczuk-Włach Joanna , Kędzierski Witold , Jonik Ilona , Sadok Ilona , Filip Agata , Kankofer Marta , Polkowski Wojciech , Ziółkowski Piotr , Gamian Andrzej , Staniszewska Magdalena
Published: April 2021

Background: Immune modulatory factors like indoleamine 2,3-dioxygenase 1 (IDO1) generating kynurenine (Kyn) and receptor for advanced glycation end-products (RAGE) contribute to endometrial and cancer microenvironment. Using adequate experimental models is needed to learn about the significance of these molecular factors in endometrial biology. In this paper we study IDO1 activity and RAGE expression in the in vitro cultured primary human endometrial cells derived from cancerous and noncancerous tissue. Methods: The generated primary cell cultures from cancer and noncancerous endometrial tissues were characterized using immunofluorescence and Western Blot for expression of endometrial and cancer markers. IDO1 activity was studied by Kyn quantification with High Performance Liquid Chromatography with Diode Array Detector. Results: The primary cultures of endometrial cells were obtained with 80% success rate and no major genetic aberrations. The cells retained in vitro expression of markers (mucin MUC1 and HER2) or immunomodulatory factors (RAGE and IDO1). Increased Kyn secretion was associated with cancer endometrial cell culture in contrast to the control one. Conclusions: Primary endometrial cells express immune modulatory factors RAGE and IDO1 in vitro associated with cancer phenotype of endometrium.
Ubiquitination, Biotech Startups, and the Future of TRIM Family Proteins: A TRIM-Endous Opportunity
Authors: Bhaduri Utsa , Merla Giuseppe
Published: April 2021

Ubiquitination is a post-translational modification that has pivotal roles in protein degradation and diversified cellular processes, and for more than two decades it has been a subject of interest in the biotech or biopharmaceutical industry. Tripartite motif (TRIM) family proteins are known to have proven E3 ubiquitin ligase activities and are involved in a multitude of cellular and physiological events and pathophysiological conditions ranging from cancers to rare genetic disorders. Although in recent years many kinds of E3 ubiquitin ligases have emerged as the preferred choices of big pharma and biotech startups in the context of protein degradation and disease biology, from a surface overview it appears that TRIM E3 ubiquitin ligases are not very well recognized yet in the realm of drug discovery. This article will review some of the blockbuster scientific discoveries and technological innovations from the world of ubiquitination and E3 ubiquitin ligases that have impacted the biopharma community, from biotech colossuses to startups, and will attempt to evaluate the future of TRIM family proteins in the province of E3 ubiquitin ligase-based drug discovery.
Progress of Spark Plasma Sintering (SPS) Method, Systems, Ceramics Applications and Industrialization
Authors: Tokita Masao
Published: April 2021

The spark plasma sintering (SPS) method is of great interest to the powder and powder metallurgy industry and material researchers of academia for both product manufacturing and advanced material research and development. Today in Japan, a number of SPS products for different industries have already been realized. Today’s fifth-generation SPS systems are capable of producing parts of increasing size, offering improved functionality, reproducibility, productivity, and cost. For instance, pure nano-Tungsten Carbide WC powder (no additives) is fully densified with a nano-grain-sized structure for glass lens application in the optics industry. The SPS is now moving from scientific academia and/or R&D proto-type materials level usage to practical industry use product stage utilizing in the field of electronics, automotive, mold and die, cutting tools, fine ceramics, clean energy, biomaterials industries, and others. This paper reviews and introduces the peculiar phenomenon of SPS and the progress of SPS technology, method, development of SPS systems, and its industrial product applications.
One-Year Clinical Aging of Low Stress Bulk-Fill Flowable Composite in Class II Restorations: A Case Report and Literature Review
Authors: Hardan Louis , Lukomska-Szymanska Monika , Zarow Maciej , Cuevas-Suárez Carlos Enrique , Bourgi Rim , Jakubowicz Natalia , Sokolowski Krzysztof , D’Arcangelo Camillo
Published: April 2021

Bulk-fill flowable composites provide functional and aesthetic restorations while eliminating incremental composite layering and saving time. The degradation of the adhesive interface with subsequent gap formation is a concern when adhesively luted restorations are placed. Moreover, the number of adhesive interface failures increases when they are exposed to long-term water storage. The aim of the present study was to evaluate the morphological characteristics of the tooth-composite interface in class II cavities restored with a low stress bulk-fill flowable composite after aging in an oral environment. We describe a case of a patient with class II cavities in four premolars restored with a low stress bulk-fill flowable composite Surefil SDR (Dentsply DeTrey GmbH, Konstanz, Germany). The occlusal part was restored with nano-hybrid resin composite Ceram X Mono (Dentsply DeTrey GmbH). After one year of clinical function, the teeth were extracted and examined in a scanning electron microscope (SEM). It can be concluded that the application of bulk-fill covered with conventional composite seems to provide the homogeneous and stable bond to tooth structure after one year of aging in an oral environment. However, some defects within the dentin-resin composite interface were observed.
Mixed Convection of Hybrid Nanofluid in an Inclined Enclosure with a Circular Center Heater under Inclined Magnetic Field
Authors: Munawar Sufian , Saleem Najma , Ahmad Khan Waqar , Nasir Sumiya
Published: April 2021

The hybrid nanofluids have efficient thermal networking due to the trade-off between the pros and cons of the more than one type of suspension. In the current study, water-based hybrid nanofluid is used to investigate mixed convection in a squared enclosure heated with a circular center heater. The cavity is placed inclined under the uniform inclined magnetic field. The squared cavity comprises of two adiabatic vertical walls and two cold horizontal walls. The governing equations are normalized using a suitable set of variables and are solved with the finite element method. A comparison is provided with previously reported results at limiting case. The grid independence is examined for the Nusselt number at the central heater. The analysis reveals the effective role of the concentration of hybrid nanofluid particles in enhancing the heat spread. The results indicate that adding 2% concentration of Ag-MgO hybrid nanoparticles causes an 18.3% uprise in the Nusselt number at the central heater. The heat transfer rate enhances for increasing Hartmann number between 0 and 10 but decreases over 10. For better heat transfer augmentation, a heater with a smaller radius is recommended for the free convection. In contrast, a heater with a larger radius serves the purpose in case of forced convection.
Comparison of Heavy Metals Removal from Aqueous Solution by Moringa oleifera Leaves and Seeds
Authors: Abatal Mohamed , Olguin M. , Anastopoulos Ioannis , Giannakoudakis Dimitrios , Lima Eder , Vargas Joel , Aguilar Claudia
Published: April 2021

In this work, biomass obtained from seeds (S-MO) and leaves (L-MO) of the Moringa oleifera plant were used as low-cost biosorbents to remove the Pb(II), Cd(II), Co(II), and Ni(II) from aqueous solutions. The biosorption of the heavy metal ions was done using the batch technique. The effects of contact time (30–1440 min), biosorbent dosage (10–50 g/L) (0.1–0.5 g), and initial concentration of metals (10–500 mg/L) on the sorption capacity of metal ions were investigated. The S-MO and L-MO samples’ characterization was performed using pHpzc, X-ray Diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR). It was found that the pHpzc was notably different between the seeds and leave-derived biosorbents. The removal process’s experimental kinetic data for both S-MO and L-MO were best described by the pseudo-second-order model for all metal ions, with R2 above 0.997 in all cases. Langmuir and Freundlich’s models were also used to analyze the isotherms parameters. Based on the Langmuir model, the maximum sorption capacities (Qm) for L-MO were found as follows: L-MO-Pb > L-MO-Cd > L-MO-Co ≥ L-MO-Ni, and for S-MO, the values of Qm values presented the following order: S-MO-Pb > S-MO-Co > S-MO-Cd > S-MO-Ni.
Employing Nanosilver, Nanocopper, and Nanoclays in Food Packaging Production: A Systematic Review
Authors: Ahari Hamed , Anvar Amir Ali , Ataee Maryam , Naeimabadi Mohammad
Published: April 2021

Over the past decade, there has been an increasing demand for “ready-to-cook” and “ready-to-eat” foods, encouraging food producers, food suppliers, and food scientists to package foods with minimal processing and loss of nutrients during food processing. Following the increasing trend in the customer’s demands for minimally processed foodstuffs, this underscores the importance of promising interests toward industrial applications of novel and practical approaches in food. Along with substantial progress in the emergence of “nanoscience”, which has turned into the call of the century, the efficacy of conventional packaging has faded away. Accordingly, there is a wide range of new types of packaging, including electronic packaging machines, flexible packaging, sterile packaging, metal containers, aluminum foil, and flexographic printing. Hence, it has been demonstrated that these novel approaches can economically improve food safety and quality, decrease the microbial load of foodborne pathogens, and reduce food spoilage. This review study provides a comprehensive overview of the most common chemical or natural nanocomposites used in food packaging that can extend food shelf life, safety and quality. Finally, we discuss applying materials in the production of active and intelligent food packaging nanocomposite, synthesis of nanomaterial, and their effects on human health.
Experimental and Numerical Study of Transient Liquid Phase Diffusion Bonded DZ40M Superalloys
Authors: Sun Zhan , Chen Xi , Zhang Lixia , Zhang Saisai , Feng Jicai
Published: April 2021

Transient liquid phase (TLP) diffusion bonding of DZ40M cobalt-based superalloy was carried out using a self-made NiCrCoWB intermediate layer. The typical microstructure of the joint was investigated. The effect of holding time on the microstructural evolution and the tensile strength of the brazed joints was studied. The tensile strength of the joints TLP bonded at 1160 °C for 60 min reached the maximum value of 487 MPa, which was 88.6% of the base metal strength. The diffusion of boron and the evolution of the eutectic zone were numerically studied. The time needed for isothermal solidification completion was calculated and predicted, which was well in accordance with the experimental results.
Design, Simulation, and Analysis of Optical Microring Resonators in Lithium Tantalate on Insulator
Authors: Yao Siyang , Han Huangpu , Jiang Shangen , Xiang Bingxi , Chai Guangyue , Ruan Shuangchen
Published: April 2021

In this paper we design, simulate, and analyze single-mode microring resonators in thin films of z-cut lithium tantalate. They operate at wavelengths that are approximately equal to 1.55 μm. The single-mode conditions and transmission losses of lithium tantalate waveguides are simulated for different geometric parameters and silica thicknesses. An analysis is presented on the quality factor and free spectral range of the microring resonators in lithium tantalate at contrasting radii and gap sizes. The electro-optical modulation performance is analyzed for microring resonators with a radius of 20 μm. Since they have important practical applications, the filtering characteristics of the microring resonators that contain two straight waveguides are analyzed. This work enhances the knowledge of lithium tantalate microring structures and offers guidance on the salient parameters for the fabrication of highly efficient multifunctional photonic integrated devices, such as tunable filters and modulators.
Malaria Rapid Diagnostic Tests: Literary Review and Recommendation for a Quality Assurance, Quality Control Algorithm
Authors: Kavanaugh Michael J. , Azzam Steven E. , Rockabrand David M.
Published: April 2021

Malaria rapid diagnostic tests (RDTs) have had an enormous global impact which contributed to the World Health Organization paradigm shift from empiric treatment to obtaining a parasitological diagnosis prior to treatment. Microscopy, the classic standard, requires significant expertise, equipment, electricity, and reagents. Alternatively, RDT’s lower complexity allows utilization in austere environments while achieving similar sensitivities and specificities. Worldwide, there are over 200 different RDT brands that utilize three antigens: Plasmodium histidine-rich protein 2 (PfHRP-2), Plasmodium lactate dehydrogenase (pLDH), and Plasmodium aldolase (pALDO). pfHRP-2 is produced exclusively by Plasmodium falciparum and is very Pf sensitive, but an alternative antigen or antigen combination is required for regions like Asia with significant Plasmodium vivax prevalence. RDT sensitivity also decreases with low parasitemia (<100 parasites/uL), genetic variability, and prozone effect. Thus, proper RDT selection and understanding of test limitations are essential. The Center for Disease Control recommends confirming RDT results by microscopy, but this is challenging, due to the utilization of clinical laboratory standards, like the College of American Pathologists (CAP) and the Clinical Lab Improvement Act (CLIA), and limited recourses. Our focus is to provide quality assurance and quality control strategies for resource-constrained environments and provide education on RDT limitations.
Diagnosis and Management of Rectal Neuroendocrine Tumors (NETs)
Authors: Maione Francesco , Chini Alessia , Milone Marco , Gennarelli Nicola , Manigrasso Michele , Maione Rosa , Cassese Gianluca , Pagano Gianluca , Tropeano Francesca Paola , Luglio Gaetano , De Palma Giovanni Domenico
Published: April 2021

Rectal neuroendocrine tumors (NETs) are rare, with an incidence of 0.17%, but they represent 12% to 27% of all NETs and 20% of gastrointestinal NETs. Although rectal NETs are uncommon tumors, their incidence has increased over the past few years, and this is probably due to the improvement in detection rates made by advanced endoscopic procedures. The biological behavior of rectal NETs may be different: factors predicting the risk of metastases have been identified, such as size and grade of differentiation. The tendency for metastatic diffusion generally depends on the tumor size, muscular and lymphovascular infiltration, and histopathological differentiation. According to the current European Neuroendocrine Tumor Society (ENETS) guidelines, tumors that are smaller than 10 mm and well differentiated are thought to have a low risk of lymphovascular invasion, and they should be completely removed endoscopically. Rectal NETs larger than 20 mm have a higher risk of involvement of muscularis propria and high metastatic risk and are candidates for surgical resection. There is controversy over rectal NETs of intermediate size, 10–19 mm, where the metastatic risk is considered to be 10–15%: assessment of tumors endoscopically and by endoanal ultrasound should guide treatment in these cases towards endoscopic, transanal, or surgical resection.
Review of Autonomous Intelligent Vehicles for Urban Driving and Parking
Authors: Chan Teck Kai , Chin Cheng Siong
Published: April 2021

With the concept of Internet-of-Things, autonomous vehicles can provide higher driving efficiency, traffic safety, and freedom for the driver to perform other tasks. This paper first covers enabling technology involving a vehicle moving out of parking, traveling on the road, and parking at the destination. The development of autonomous vehicles relies on the data collected for deployment in actual road conditions. Research gaps and recommendations for autonomous intelligent vehicles are included. For example, a sudden obstacle while the autonomous vehicle executes the parking trajectory on the road is discussed. Several aspects of social problems, such as the liability of an accident affecting the autonomous vehicle, are described. A smart device to detect abnormal driving behaviors to prevent possible accidents is briefly discussed.
A Reversible Watermarking System for Medical Color Images: Balancing Capacity, Imperceptibility, and Robustness
Authors: Zhou Xiaoyi , Ma Yue , Zhang Qingquan , Mohammed Mazin Abed , Damaševičius Robertas
Published: April 2021

The authenticity and integrity of medical images in telemedicine has to be protected. Robust reversible watermarking (RRW) algorithms provide copyright protection and the original images can be recovered at the receiver’s end. However, the existing algorithms have limitations in their ability to balance the tradeoff among robustness, imperceptibility, and embedded capacity. Some of them are even not completely reversible. Besides, most medical image watermarking algorithms are not designed for color images. To improve their performance in protecting medical color image information, we propose a novel RRW scheme based on the discrete wavelet transform (DWT). First, the DWT provides a robust solution. Second, the modification of the wavelet domain coefficient guarantees the changes of integer values in the spatial domain and ensures the reversibility of the watermarking scheme. Third, the embedding scheme makes full use of the characteristics of the original image and watermarking. This reduces the modification of the original image and ensures better imperceptibility. Lastly, the selection of the Zernike moments order for geometric correction is optimized to predict attack parameters more accurately by using less information. This enhances the robustness of the proposed scheme against geometric attacks such as rotation and scaling. The proposed scheme is robust against common and geometric attacks and has a high embedding capacity without obvious distortion of the image. The paper contributes towards improving the security of medical images in remote healthcare.
Multi-Behavior with Bottleneck Features LSTM for Load Forecasting in Building Energy Management System
Authors: Bui Van , Le Nam Tuan , Nguyen Van Hoa , Kim Joongheon , Jang Yeong Min
Published: April 2021

With the wide use of the Internet of Things and artificial intelligence, energy management systems play an increasingly important role in the management and control of energy consumption in modern buildings. Load forecasting for building energy management systems is one of the most challenging forecasting tasks as it requires high accuracy and stable operating conditions. In this study, we propose a novel multi-behavior with bottleneck features long short-term memory (LSTM) model that combines the predictive behavior of long-term, short-term, and weekly feature models by using the bottleneck feature technique for building energy management systems. The proposed model, along with the unique scheme, provides predictions with the accuracy of long-term memory, adapts to unexpected and unpatternizable intrinsic temporal factors through the short-term memory, and remains stable because of the weekly features of input data. To verify the accuracy and stability of the proposed model, we present and analyze several learning models and metrics for evaluation. Corresponding experiments are conducted and detailed information on data preparation and model training are provided. Relative to single-model LSTM, the proposed model achieves improved performance and displays an excellent capability to respond to unexpected situations in building energy management systems.
Biochemical Basis of Altitude Adaptation and Antioxidant System Activity during Autumn Leaf Senescence in Beech Populations
Authors: Kraj Wojciech , Zarek Marcin
Published: April 2021

High-altitude tree populations are exposed to severe natural environmental conditions. Among abiotic factors, variable temperatures, early frosts, and high radiation are the factors affecting tree growth at high altitudes. Fagus sylvatica L. exhibits a variety of physiological and genetic traits that allow it to adapt to different forest habitats. This study examines the differences in the biochemical properties of senescing beech leaves between populations originating from different altitudes using a common-garden experiment. Leaves were collected from five-year-old plants from the beginning of August to the end of October for two years. Based on the changes in senescence marker levels the genetic differences and significant correlations between populations’ altitude origin and their biochemical characteristics were identified. According to the free radical theory of leaf senescence, reactive oxygen species (ROS) and senescence markers were highly correlated. In this study, populations from higher altitudes were characterized by earlier and greater increases in ROS content and oxidative stress, which resulted in higher antioxidative system activity. Increases in ROS in high-altitude populations play a controlling role to initiate earlier senescence processes that allow the trees to adapt to harsh climatic conditions. Earlier senescence allows beech trees to maintain a balance between nitrogen metabolism and photosynthetic activity. It allows for remobilization of nitrogen compounds more efficiently and protects the trees from nitrogen loss and prepares them for winter dormancy.
Mechanical Properties of Machine Stress Graded Sawn Timber Depending on the Log Type
Authors: Burawska-Kupniewska Izabela , Mańkowski Piotr , Krzosek Sławomir
Published: April 2021

This article presents the results of tests conducted on Scots pine timber from three different kinds of logs: butt, middle and top. A planed pine timber batch composed of 510 pieces, dried to ca. 12% humidity, was machine-graded using a portable MTG device, and classified into the following classes: C18, C24, C30, C35 and C40 (according to EN 338:2016). During the second stage of the study, the timber was tested to determine its density, MOE and MOR, in accordance with EN 408:2012. We analyzed the impact of the timber’s log of origin on the results of machine strength grading and on the values of correlation coefficients between the tested properties. The results show, among others, that there is a correlation between the C classes and MOR of the tested timber, as well as its origin from butt, middle or top logs.
Mutual Influence of Users Credibility and News Spreading in Online Social Networks
Authors: Carchiolo Vincenza , Longheu Alessandro , Malgeri Michele , Mangioni Giuseppe , Previti Marialaura
Published: April 2021

A real-time news spreading is now available for everyone, especially thanks to Online Social Networks (OSNs) that easily endorse gate watching, so the collective intelligence and knowledge of dedicated communities are exploited to filter the news flow and to highlight and debate relevant topics. The main drawback is that the responsibility for judging the content and accuracy of information moves from editors and journalists to online information users, with the side effect of the potential growth of fake news. In such a scenario, trustworthiness about information providers cannot be overlooked anymore, rather it more and more helps in discerning real news from fakes. In this paper we evaluate how trustworthiness among OSN users influences the news spreading process. To this purpose, we consider the news spreading as a Susceptible-Infected-Recovered (SIR) process in OSN, adding the contribution credibility of users as a layer on top of OSN. Simulations with both fake and true news spreading on such a multiplex network show that the credibility improves the diffusion of real news while limiting the propagation of fakes. The proposed approach can also be extended to real social networks.
Insight into the Genetic Population Structure of Wild Red Foxes in Poland Reveals Low Risk of Genetic Introgression from Escaped Farm Red Foxes
Authors: Wierzbicki Heliodor , Zatoń-Dobrowolska Magdalena , Mucha Anna , Moska Magdalena
Published: April 2021

In this study we assessed the level of genetic introgression between red foxes bred on fur farms in Poland and the native wild population. We also evaluated the impact of a geographic barrier and isolation by distance on gene flow between two isolated subpopulations of the native red fox and their genetic differentiation. Nuclear and mitochondrial DNA was collected from a total of 308 individuals (200 farm and 108 wild red foxes) to study non-native allele flow from farm into wild red fox populations. Genetic structure analyses performed using 24 autosomal microsatellites showed two genetic clusters as being the most probable number of distinct populations. No strong admixture signals between farm and wild red foxes were detected, and significant genetic differentiation was identified between the two groups. This was also apparent from the mtDNA analysis. None of the concatenated haplotypes detected in farm foxes was found in wild animals. The consequence of this was that the haplotype network displayed two genetically distinct groups: farm foxes were completely separated from native ones. Neither the River Vistula nor isolation by distance had a significant impact on gene flow between the separated wild red fox subpopulations. The results of our research indicate a low probability of genetic introgression between farm and native red foxes, and no threat to the genetic integrity of this species.
The Alps-Apennines Interference Zone: A Perspective from the Maritime and Western Ligurian Alps
Authors: Piana Fabrizio , Barale Luca , Bertok Carlo , d’Atri Anna , Irace Andrea , Mosca Pietro
Published: April 2021

In SW Piemonte the Western Alps arc ends off in a narrow, E-W trending zone, where some geological domains of the Alps converged. Based on a critical review of available data, integrated with new field data, it is concluded that the southern termination of Western Alps recorded the Oligocene-Miocene activity of a regional transfer zone (southwestern Alps Transfer, SWAT) already postulated in the literature, which should have allowed, since early Oligocene, the westward indentation of Adria, while the regional shortening of SW Alps and tectonic transport toward the SSW (Dauphinois foreland) was continuing. This transfer zone corresponds to a system of deformation units and km-scale shear zones (Gardetta-Viozene Zone, GVZ). The GVZ/SWAT developed externally to the Penninic Front (PF), here corresponding to the Internal Briançonnais Front (IBF), which separates the Internal Briançonnais domain, affected by major tectono-metamorphic transformations, from the External Briançonnais, subjected only to anchizonal metamorphic conditions. The postcollisional evolution of the SW Alps axial belt units was recorded by the Oligocene to Miocene inner syn-orogenic basin (Tertiary Piemonte Basin, TPB), which rests also on the Ligurian units stacked within the adjoining Apennines belt in southern Piemonte. The TPB successions were controlled by transpressive faults propagating (to E and NE) from the previously formed Alpine belt, as well as by the Apennine thrusts that were progressively stacking the Ligurian units, resting on the subducting Adriatic continental margin, with the TPB units themselves. This allows correlation between Alps and Apennines kinematics, in terms of age of the main geologic events, interference between the main structural systems and tectonic control exerted by both tectonic belts on the same syn-orogenic basin.
COVID-19 Perceived Impact and Psychological Variables as Predictors of Unhealthy Food and Alcohol Consumption Trajectories: The Role of Gender and Living with Children as Moderators
Authors: Salazar-Fernández Camila , Palet Daniela , Haeger Paola A. , Román Mella Francisca
Published: April 2021

The present study examines the trajectories of unhealthy food and alcohol consumption over time and considers whether perceived impact of COVID-19 and psychological variables are predictors of these trajectories. We ascertained whether these predictors are different in women vs. men and between women living with vs. without children. Data were collected through online surveys administered to 1038 participants from two universities (staff and students) in Chile, across five waves (July to October 2020). Participants provided information about their past-week unhealthy food and alcohol consumption and mental health. Using latent growth curve modeling analysis, we found that higher perceived health and interpersonal COVID-19 impact, younger age and lower depression symptoms were associated with more rapid increases over time in unhealthy food consumption. On the other hand, higher perceived COVID-19 economic impact and older age were associated with more rapid diachronic decreases in alcohol consumption. Gender and living with or without children, for women only, were moderators of these trajectories. This longitudinal study provides strong evidence identifying the multiple repercussions of COVID-19 and mental health factors on unhealthy food and alcohol consumption. These findings highlight the need for interventions aimed at minimizing the impact of the pandemic on unhealthy food and alcohol consumption over time.
Colorectal Cancer among Resettlers from the Former Soviet Union and in the General German Population: Clinical and Pathological Characteristics and Trends
Authors: Mahanani Melani Ratih , Kaucher Simone , Kajüter Hiltraud , Holleczek Bernd , Becher Heiko , Winkler Volker
Published: April 2021

This study examined time trends and clinical and pathological characteristics of colorectal cancer (CRC) among ethnic German migrants from the Former Soviet Union (resettlers) and the general German population. Incidence data from two population-based cancer registries were used to analyze CRC as age-standardized rates (ASRs) over time. The respective general populations and resettler cohorts were used to calculate standardized incidence ratios (SIRs) by time-period (before and after the introduction of screening colonoscopy in 2002), tumor location, histologic type, grade, and stage at diagnosis. Additionally, SIRs were modeled with Poisson regression to depict time trends. During the study period from 1990 to 2013, the general populations showed a yearly increase of ASR, but for age above 55, truncated ASR started to decline after 2002. Among resettlers, 229 CRC cases were observed, resulting in a lowered incidence for all clinical and pathological characteristics compared to the general population (overall SIR: 0.78, 95% CI 0.68–0.89). Regression analysis revealed an increasing SIR trend after 2002. Population-wide CRC incidence decreases after the introduction of screening colonoscopy. In contrast the lowered CRC incidence among resettlers is attenuating to the general population after 2002, suggesting that resettlers do not benefit equally from screening colonoscopy.
Carrying Asymmetric Loads While Walking on a Treadmill Interferes with Lower Limb Coordination
Authors: Wang Junsig , Stephenson Mitchell L. , Hass Chris J. , Janelle Christopher M. , Tillman Mark D.
Published: April 2021

The purpose of this study was to investigate the effect of different load carriage modes on coordinative patterns in the lower extremities during walking. Twenty-five university students walked on a treadmill at their preferred pace under three different load conditions: symmetric load (5% of body mass in messenger bags on each shoulder hanging vertically and against the hips), asymmetric load 1 (10% of body mass in a messenger bag on one shoulder hanging vertically against the ipsilateral hip), and asymmetric load 2 (10% of body mass in a messenger bag on one shoulder with the bag draped across the trunk to the contralateral hip). Altered thigh-shank and shank-foot couplings were found for the loaded side during the stance of gait when comparing the asymmetric 1 and 2 to the symmetric load. In addition, thigh-thigh coupling was changed during gait when comparing the asymmetric load 2 and symmetric load. However, we did not find any significant differences in intralimb and interlimb couplings between the two different asymmetric load conditions. The results suggest potential benefits when carrying symmetrical loads in order to decrease abnormal limb coordination in daily activities. Thus, it may be advisable to distribute load more symmetrically to avoid abnormal gait.
Relationship of Sodium Intake with Overweight/Obesity among Chinese Children and Adolescents: Data from the CNNHS 2010–2012
Authors: Fang Kehong , He Yuna , Fang Yuehui , Lian Yiyao
Published: April 2021

This study aims to examine association between sodium intake and overweight/obesity among Chinese children and adolescents. Data were obtained from China National Nutrition and Health Surveillance (CNNHS), 2010–2012. All participants recruited in this study aged 7–18 years old and provided complete dietary data on three-day consecutive 24 h dietary recalls combining with the household weighing method. Body Mass Index (BMI) was used to define overweight/obesity, and waist-to-height ratio (WHtR) was used to define abdominal obesity. Sodium intake showed association with risk of overweight/obesity assessed by BMI in the highest tertile group with OR of 1.48 (95%CI 1.13–1.94) and 1.89 (95%CI 1.33–2.67) for WHtR. After adjusted for gender, age, household income, area, energy, carbohydrates, protein, fat, saturated fatty acids, and fiber intake, the relationship between sodium intake and overweight/obesity and abdominal obesity are not changed. The same results were founded in subjects aged 10–18 years old. Our results reveal a positive association between sodium intake and overweight/obesity in Chinese children and adolescents, independent of energy consumption.
The Impact of COVID-19 on Sport in Twitter: A Quantitative and Qualitative Content Analysis
Authors: González Luis-Millán , Devís-Devís José , Pellicer-Chenoll Maite , Pans Miquel , Pardo-Ibañez Alberto , García-Massó Xavier , Peset Fernanda , Garzón-Farinós Fernanda , Pérez-Samaniego Víctor
Published: April 2021

The spread of the SARS-CoV-2 virus has transformed many aspects of people’s daily life, including sports. Social networks have been flooded on these issues. The present study aims to analyze the tweets produced relating to sports and COVID-19. From the end of January to the beginning of May 2020, over 4,000,000 tweets on this subject were downloaded through the Twitter search API. Once the duplicates, replicas, and retweets were removed, 119,253 original tweets were analyzed. A quantitative–qualitative content analysis was used to study the selected tweets. Posts dynamics regarding sport and exercise evolved according to the COVID-19 pandemic and subsequent lockdown, shifting from considering sport as a healthy bastion to an activity exposed to disease like any other. Most media professional sporting events received great attention on Twitter, while grassroots and women’s sport were relegated to a residual role. The analysis of the 30 topics identified focused on the social, sporting, economic and health impact of the pandemic on the sport. Sporting cancellations, leisure time and socialization disruptions, club bankruptcies, sports training and athletes’ uncertain career development were the main concerns. Although general health measures appeared in the tweets analyzed, those addressed to sports practice were relatively scarce. Finally, this study shows the importance of Twitter as a means of conveying social attitudes towards sports and COVID-19 and its potential to generate alternative responses in future stages of the pandemic.