bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical feasibility of Ethos auto-segmentation for adaptive whole-breast cancer treatment
Authors: Prunaretty Jessica , Mekki Fatima , Laurent Pierre-Ivan , Morel Aurelie , Hinault Pauline , Bourgier Celine , Azria David , Fenoglietto Pascal
Published: December 2024

Introduction

Following a preliminary work validating the technological feasibility of an adaptive workflow with Ethos for whole-breast cancer, this study aims to clinically evaluate the automatic segmentation generated by Ethos.

Material and methods

Twenty patients initially treated on a TrueBeam accelerator for different breast cancer indications (right/left, lumpectomy/mastectomy) were replanned using the Ethos® emulator. The adaptive workflow was performed using 5 randomly selected extended CBCTs per patient. The contours generated by artificial intelligence (AI) included both breasts, the heart, and the lungs. The target volumes, specifically the tumor bed (CTV_Boost), internal mammary chain (CTV_IMC), and clavicular nodes (CTV_Nodes), were generated through rigid propagation. The CTV_Breast corresponds to the ipsilateral breast, excluding 5mm from the skin. Two radiation oncologists independently repeated the workflow and qualitatively assessed the accuracy of the contours using a scoring system from 3 (contour to be redone) to 0 (no correction needed). Quantitative evaluation was carried out using the Dice Similarity Coefficient (DSC), Hausdorff Distance (HD), surface Dice (sDSC) and the Added Path Length (APL). The interobserver variability (IOV) between the two observers was also assessed and served as a reference. Lastly, the dosimetric impact of contour correction was evaluated. The physician-validated contours were transferred onto the plans automatically generated by Ethos in adaptive mode. The dose prescription was 52.2Gy in 18 fractions for the boost, 42.3Gy for the breast, IMC, and nodes. The CTV/PTV margin was 2mm for all volumes, except for the IMC (5mm). Dose coverage (D98%) was assessed for the CTVs, while specific parameters for organs at risk (OAR) were evaluated: mean dose and V17Gy (relative volume receiving at least 17Gy) for the ipsilateral lung, mean dose and D2cc (dose received by 2cc volume) for the heart, the contralateral lung and breast.

Results

The qualitative analysis showed that no correction or only minor corrections were needed for 98.6% of AI-generated contours and 86.7% of the target volumes. Regarding the quantitative analysis, Ethos’ contour generation outperformed inter-observer variability for all structures in terms of DSC, HD, sDSC and APL. Target volume coverage was achieved for 97.9%, 96.3%, 94.2% and 68.8% of the breast, IMC, nodes and boost CTVs, respectively. As for OARs, no significant differences in dosimetric parameters were observed.

Conclusion

This study shows high accuracy of segmentation performed by Ethos for breast cancer, except for the CTV_Boost. Contouring practices for adaptive sessions were revised following this study to improve outcomes.
Prophylactic ondansetron for preventing intraoperative shivering, nausea and vomiting during spinal anesthesia for cesarean section: a randomized controlled trial
Authors: Zhang Yuan , Xia Fen , Zhang Wangping , Lv Anqing
Published: December 2024

Background

Shivering, nausea and vomiting are common complications in cesarean section during neuraxial anesthesia. The aim of this study was to investigate the effects of prophylactic use of ondansetron on intraoperative shivering, nausea and vomiting in women undergoing cesarean section.

Methods

A total of 80 full-term parturients were randomly divided into the ondansetron group and the control group. The ondansetron group received 0.075 mg/kg of ondansetron 15 min before anesthesia, the control group were given the same volume of saline solution. The incidence of shivering, nausea and vomiting was noted. The occurrence and severity of shivering and other outcomes, such as hypotension, bradycardia and dizziness were recorded during the surgery. Umbilical arterial blood was analyzed, and the neonatal Apgar scores were assessed.

Results

The incidence of grade ≥1 shivering was lower in ondansetron group. The incidence of shivering, nausea and vomiting was significantly lower in ondansetron group than the control group (2.5% vs. 22.3%, P = 0.007, 2.5% vs. 40%, P = 0.001, respectively). No significant differences were observed in the incidence of hypotension, bradycardia, headache and dizziness between the 2 groups (P > 0.05). The umbilical artery pH and neonatal Apgar score were similar between the 2 groups (P > 0.05).

Conclusion

Prophylactic use of ondansetron could prevent intra-operative shivering and reduce the incidence of nausea and vomiting in cesarean section under spinal anesthesia without increasing the incidence of maternal and infant adverse events.

Clinical Trial Registration

https://www.chictr.org.cn, identifier ChiCTR2100042453
Enhancing maize drought and heat tolerance: single vs combined plant growth promoting rhizobacterial inoculation
Authors: Notununu Iviwe , Moleleki Lucy , Roopnarain Ashira , Adeleke Rasheed
Published: December 2024

Maize (Zea mays L.), a key staple crop in Sub-Saharan Africa, is particularly vulnerable to concurrent drought and heat stress, which threatens crop yield and food security. Plant growth-promoting rhizobacteria (PGPR) have shown potential as biofertilizers to enhance plant resilience under such abiotic stresses. This study aimed to (1) identify PGPR isolates tolerant to drought and heat, (2) assess their capacity to mitigate the effects of these stresses on early maize growth, and (3) analyze maize gene expression changes associated with PGPR-induced tolerance. Rhizobacteria were isolated and screened for drought and heat tolerance, alongside key plant growth-promoting (PGP) traits, including phosphorus solubilization, nitrogen fixation, and indole acetic acid production. In vitro and pot trials evaluated the effects of selected isolates on maize growth under stress, using indicators such as shoot length, root and shoot biomass (wet and dry), and leaf water content. Quantitative reverse transcription PCR (qRT-PCR) was employed to profile maize stress response genes. The identified PGPR isolates included Bacillus cereus (11MN1), Bacillus pseudomycoides (21MN1B), Lelliottia amnigena (33MP1), and Leclercia adecarboxylata (36MP8). Greenhouse trials demonstrated that L. amnigena 33MP1, L. adecarboxylata 36MP8, and a mixed culture of isolates (11MN1, 21MN1B, 33MP1, 36MP8) effectively alleviated the adverse effects of concurrent drought and heat stress in maize. Notably, qRT-PCR analysis indicated that PGPR-induced tolerance may involve the modulation of stress response genes CAT2 (catalase 2) and DHN2 (dehydrin 2), which play roles in oxidative stress management and cellular protection. The PGPR isolates identified in this study represent promising bioinoculants for enhancing maize resilience under climate-induced stresses, offering a sustainable approach to improve maize productivity, conserve water, and reduce irrigation needs in drought-prone regions.
Genome-wide identification and characterization of BASIC PENTACYSTEINE transcription factors and their binding motifs in coconut palm
Authors: Lao Zifen , Mao Jiali , Chen Runan , Xu Ran , Yang Zhuang , Wang Ying , Zhou Junjie , Mu Zhihua , Xu Hang , Li Fengmei , Huang Dongyi , Xiao Yong , Luo Jie , Xia Wei
Published: December 2024

Introduction

BASIC PENTACYSTEINE (BPC) is a small transcription factor family known for its role in various developmental processes in plants, particularly in binding GA motifs and regulating flower and seed development. However, research on the functional characteristics and target genes of BPCs in coconut (Cocos nucifera) is limited.

Methods

In this study, we systematically characterized the gene structure, conserved protein domains, gene expansion, and target genes of CnBPCs in the coconut genome. We conducted yeast one-hybrid (Y1H) and dual-luciferase assay to explore gene interactions. We identified genes with the GA motif in their promoter regions and combined this information with a weighted gene co-expression network to identify the target genes of CnBPCs.

Results

Eight CnBPCs were identified, including three Class I CnBPCs from triplication, four Class II CnBPCs (with CnBPC6A and CnBPC6B resulting from segmental duplication), and one Class III CnBPC (CnBPC7). Three conserved DNA-binding motifs were detected, exhibiting variation in certain sites. Widespread BPC gene expansion was detected in coconut and other plant species, while only three BPCs were found in the most basal extant flowering plant. Notably, 92% of protein-coding genes contained at least one GA motif, with the (GA)3 motif being most prevalent. Genes containing the GA motif that exhibit a high expression correlation with CnBPCs, tend to interact strongly with the corresponding CnBPCs. Additionally, promoters rich in the GA motif tend to interact with all members of CnBPC. The dual-luciferase assay showed that CnBPCs could activate or repress the transcriptional activities of promoters containing either (GA)3 or (GA)11 motif but with a bias toward certain genes. Furthermore, we constructed co-expressed networks identifying 426 genes with GA motifs as potential CnBPC targets.

Discussion

Our findings suggest that CnBPCs may play significant roles in seed germination, flower development, and mesocarp development by interacting with genes such as CnAG1, CnAG2, CnSTK, CnMFT, and CnCS. This study characterized CnBPCs’ binding motif and possible target genes, laying a theoretical foundation to reveal CnBPCs’ function in flower and seed development.
The relationship between perfectionism and marital outcomes: a systematic review and meta-analysis
Authors: Hadian Hamedani Kiana , Majzoobi Mohammad Reza , Forstmeier Simon
Published: December 2024

Introduction

Perfectionism, as a transdiagnostic variable, can influence both the intrapersonal and interpersonal domains, one of the most significant of them is thought to be marital relationship. Given that perfectionism within a couple can negatively affect their intimate relationship and potentially lead to destructive outcomes, reviewing studies conducted in this area seems to be essential to gain a clearer understanding. Therefore, the present systematic review aims to examine the association between perfectionism and marital outcomes in married individuals.

Methods

A comprehensive search was conducted across major scientific databases, including APA PsycArticles, PubMed, and Web of Science, using specific keywords and Boolean operators. Included were the English-Language studies published between 1980 and 2023 that investigated the relationship between perfectionism and marital outcomes in heterosexual couples. Out of the studies identified, 23 met the inclusion criteria for this review, of which 16 studies met the criteria for meta-analysis.

Results

The meta-analysis indicated a small-to-moderate effect size for the association between perfectionism and marital outcomes (r = 0.26). Given the importance of marital relationship, such effect sizes for variables which may influence marital relationship bears significant value.

Discussion

Therefore, the findings may encourage researchers to conduct various studies to examine specific details, moderators, and mediators in the relationship between perfectionism and marital outcomes and may also prompt couple therapists to address perfectionism as a destructive factor and integrate strategies into their protocols to reduce its impact in intimate relationships.
Increased risk of injury and adult attention deficit hyperactivity disorder and effects of pharmacotherapy: a nationwide longitudinal cohort study in South Korea
Authors: Ahn Jaeun , Shin Jae-won , Park Haeyong , Ha Joong-Won
Published: December 2024

Children and adolescents with attention deficit hyperactivity disorder (ADHD) are at an increased risk of accidents and injuries, and ADHD medication has been shown to mitigate this risk in these populations. However, the injury risk and the influence of ADHD medication in adults with ADHD remain unclear. This study aimed to investigate the injury risk in adults with ADHD and assess the impact of ADHD medication on this risk. Using a nationwide health claims database, we identified 9,417 adult patients with ADHD aged 19–44 years between 2017 and 2018. A retrospective propensity score-matched case-control study was conducted to examine the association between adult ADHD and injury risk across various categories. The effects of two commonly prescribed ADHD medications, methylphenidate and atomoxetine, were evaluated using a Cox proportional hazards model. The results showed that adults with ADHD had an increased risk of sustaining various types of injuries. Methylphenidate demonstrated a protective effect against injury, which persisted after adjusting for potential confounding factors. Similarly, atomoxetine significantly reduced the injury risk. These findings underscore the importance of injury prevention strategies in adults with ADHD and highlight the substantial health benefits of ADHD medications in this population.
Iowa Gambling Task performance in individuals with schizophrenia: the role of general versus specific cognitive abilities
Authors: Orm Stian , Øie Merete Glenne , Haugen Ingvild
Published: December 2024

Objective

We aimed to explore how specific cognitive processes, such as attention and executive functions, account for variance in decision-making measured by Iowa Gambling Task (IGT) performance among individuals with schizophrenia spectrum disorders.

Methods

Adults (N = 65, Mage = 25.4) with schizophrenia spectrum disorders participating in a clinical trial (registered at clinicaltrials.gov NCT03048695) completed the IGT, neuropsychological tests of attention, response inhibition, mental flexibility, working memory, and planning, as well as subtests from the Wechsler tests of intelligence to estimate IQ. Associations between performance on specific tasks, a composite score of executive function and attention, and IGT performance measured in two ways, one using the total net score, decks (C+D) – (A+B) and the other as preference for decks with more frequent gains than losses, decks (B+D) – (A+C), were analyzed with correlational and hierarchical regression analysis controlling for estimated IQ and psychotic symptoms, measured by the Positive and Negative Syndrome Scale.

Results

In the regression analyses, the strongest predictor of IGT performance measured as the total net score was estimated IQ (b = 1.43, p <.001). Neither specific cognitive tasks nor the composite score of executive functioning significantly contributed to explaining variance in IGT total net score beyond IQ and symptoms of psychosis. However, IQ and symptoms of psychosis did not predict tendency towards selecting decks with different gain-to-loss frequency, whereas poorer composite executive functioning predicted a pattern of selecting decks A and C with more frequent losses, (b = 8.30, p <.05).

Discussion

The results suggest that both IQ and executive functions contribute to IGT performance, but in distinct ways. Whereas lower IQ may contribute to overall more disadvantageous decision-making, poorer executive functioning may contribute to a more risk-aversive decision-making style. A clinical implication may be that individuals with schizophrenia and lower IQ or poorer executive functioning will have a higher need for support and interventions targeting decision-making.
Stretching after spinal cord injury: a call for evidence for this common clinical practice
Authors: Williams Todd E. , DeMark Louis A. , Olarewaju Tinuade A. , Hawkins Kelly A. , Fox Emily J.
Published: December 2024

Stretching is a ubiquitous rehabilitation intervention for individuals with spinal cord injury (SCI), intended to reduce spasticity, maintain or improve joint range of motion, and prevent joint contractures. Although people with SCI report that stretching is their preferred approach to reduce spasticity, limited evidence supports the use of stretching for people with SCI, including short-term (< one hour) effects on spasticity. Further, the long-term effects and the effects of stretching on motor function have yet to be examined in humans with SCI. Evidence from pre-clinical studies in rats with SCI demonstrates that stretching impairs motor output, reduces spinal cord excitability, and abolishes walking function. This perspective paper discusses evidence of static stretching in humans and rats with SCI regarding the effects on range of motion, joint contractures, and effects on voluntary and involuntary (i.e., spasticity) motor output. Additionally, we aim to challenge assumptions regarding the use of stretching and encourage research to advance the understanding of this common rehabilitation approach. Research is needed to investigate underlying mechanisms of stretch-induced effects and to advance stretching protocols to optimize the potential beneficial effects of stretching for people with SCI.
Coupling coordination measurement and spatiotemporal evolution characteristics of digital economy, environmental regulation, and carbon emission intensity—Empirical evidence from Chinese provinces
Authors: Tang Shibo
Published: December 2024

Digital economy, as an innovative digital technology, can promote environmental governance and support low-carbon development. Under the backdrop of China's dual carbon strategy, the country faces significant challenges in achieving coordinated development across the domains of economy, environment, and energy. Based on panel data from 30 Chinese provinces between 2012 and 2022, this paper constructs a coupling coordination model to measure the level of integrated development among digital economy, environmental regulation, and carbon emission intensity. The study also examines the spatiotemporal evolution of this coordination using kernel density estimation and spatial correlation tests. Furthermore, empirical analysis via regression models was employed to investigate key influencing factors. The findings suggest that: (1) The coupling coordination level has shown an upward trend over the sample period, with no extreme regional disparities; (2) Spatially, the development of coupling coordination exhibits “high-high” and “low-low” clustering effects; (3) Development levels are influenced by various external driving factors, with significant regional differences in primary influencing factors. For these findings, the following recommendations are proposed: enhance digital infrastructure, optimize regulatory frameworks, and promote the integration of digital economy with green and low-carbon development.
Oral pre-exposure prophylaxis implementation in South Africa: a case study of USAID-supported programs
Authors: Milimu Jerome Wendoh , Parmley Lauren , Matjeng Mahlodi , Madibane Mathata , Mabika Mandisi , Livingston Jacques , Lawrence Joseph , Motlhaoleng Orapeleng , Subedar Hasina , Tsekoa Rethabile , Mthembu Zandile
Published: December 2024

Since the introduction of oral pre-exposure prophylaxis (PrEP) in 2016, countries have successfully scaled-up PrEP to populations at risk of HIV acquisition, including key populations, serodiscordant couples and pregnant women. Between 2016 and 2023, there were over 5.6 million oral PrEP initiations globally. Of these, over 1.2 million occurred in South Africa, with nearly 700,000 implemented through USAID/South Africa's PEPFAR program. This case study uses WHO's Building Blocks for Health Systems Strengthening to describe USAID's oral PrEP program in South Africa, reporting experiences and lessons learned in 14 districts across 7 provinces. Key lessons include: (i) Substantial donor financial investment was critical for expanding oral PrEP in South Africa, but sustained leadership and investment from government stakeholders, such as the Department of Health and the National Treasury, have been essential for sustainability. Despite fluctuations in USAID funding, annual PrEP initiations have continued to increase in USAID-supported districts largely due to local leadership. (ii) Health information and supply chain systems required agility to monitor oral PrEP introduction and scale-up. When systems lacked agility, temporary solutions like the development of interim reporting tools were necessary. (iii) Integrating community-based and facility-based service delivery supported client-centered care. Nurses and lay health workers contributed to over 80% of the full-time equivalents supporting PrEP under USAID's human resources for health portfolio. (iv) Integrating sexual and reproductive health services with oral PrEP service delivery provided clients with comprehensive, client-centered care. (v) Other client-centered care included differentiated service delivery options, such as mobile and gazebo modalities, and expanded PrEP choice through implementation science activities for new PrEP products. (vi) USAID-supported PrEP initiations have been highest among females of reproductive age in the general population and men who have sex with men among key populations, priority populations in South Africa. As done in this case study, sharing best practices and lessons learned from USAID/South Africa's oral PrEP program can strengthen the implementation evidence base and inform more efficient PrEP service delivery, particularly as new PrEP products become available.
Optimizing bone transport strategies: a pixel value ratio-based evaluation of regeneration rates in bifocal and trifocal techniques
Authors: Yang Xin , Hamiti Yimurang , Liu Kai , Wang Sulong , Kadier Xiriaili , Xiong Debin , Yusufu Aihemaitijiang
Published: December 2024

Background

Bone transport techniques are crucial for managing large bone defects, but the optimal approach for different defect lengths remains unclear. This study aimed to compare bone regeneration rates between short bifocal bone transport (SBBT), long bifocal bone transport (LBBT), and trifocal bone transport (TBT) using pixel value ratio (PVR) as an objective quantitative measure.

Methods

This retrospective study included 60 patients undergoing lower limb bone transport, divided into SBBT (n = 22, defects <6 cm), LBBT (n = 20, defects ≥6 cm), and TBT (n = 18, defects ≥6 cm) groups. PVR was measured at 4, 8, and 12 weeks postoperatively using standardized digital radiographs. Healing index (HI) and external fixation index (EFI) were calculated to assess treatment efficiency. Demographic data, surgical characteristics, and complications were also analyzed.

Results

TBT showed significantly higher PVR values compared to LBBT at all time points (4 weeks: 0.779 ± 0.036 vs. 0.719 ± 0.027, p < 0.001; 8 weeks: 0.822 ± 0.027 vs. 0.787 ± 0.025, p = 0.008; 12 weeks: 0.866 ± 0.024 vs. 0.835 ± 0.016, p = 0.023) and to SBBT at 4 and 8 weeks (p < 0.001 and p = 0.016, respectively). The TBT group demonstrated significantly lower HI and EFI compared to both SBBT and LBBT groups (p < 0.05), indicating faster healing and shorter treatment times. Although SBBT showed slightly higher PVR values than LBBT, the differences were not statistically significant.

Conclusion

Trifocal bone transport leads to faster bone regeneration and shorter treatment times compared to bifocal techniques, particularly for longer bone defects. The study demonstrates that defect length alone may not be the primary factor influencing regeneration rates in bifocal transport. PVR proves to be a reliable and cost-effective tool for assessing bone regeneration in different bone transport techniques, offering potential for guiding clinical decision-making. These findings suggest that trifocal transport should be considered as a preferred method for treating larger bone defects, especially when minimizing treatment time is crucial.
Exploratory Study of a Green Function Based Solver for Nonlinear Partial Differential Equations
Authors: Solano-López Pablo , Saavedra Jorge , Molina Raúl
Published: December 2024

This work explores the numerical translation of the weak or integral solution of nonlinear partial differential equations into a numerically efficient, time-evolving scheme. Specifically, we focus on partial differential equations separable into a quasilinear term and a nonlinear one, with the former defining the Green function of the problem. Utilizing the Green function under a short-time approximation, it becomes possible to derive the integral solution of the problem by breaking it into three integral terms: the propagation of initial conditions and the contributions of the nonlinear and boundary terms. Accordingly, we follow this division to describe and separately analyze the resulting algorithm. To ensure low interpolation error and accurate numerical Green functions, we adapt a piecewise interpolation collocation method to the integral scheme, optimizing the positioning of grid points near the boundary region. At the same time, we employ a second-order quadrature method in time to efficiently implement the nonlinear terms. Validation of both adapted methodologies is conducted by applying them to problems with known analytical solution, as well as to more challenging, norm-preserving problems such as the Burgers equation and the soliton solution of the nonlinear Schrödinger equation. Finally, the boundary term is derived and validated using a series of test cases that cover the range of possible scenarios for boundary problems within the introduced methodology.
Strategies for Regulating the Bioavailability and Mobility of Se and Cd in Cd-Contaminated Seleniferous Soils: Coupling the Bioavailable Se:Cd Molar Ratio with Soil Properties
Authors: Tan Zebin , Rong Qinlei , Wang Wenfeng , Jiang Haiyan , Yu Luyao , Hu Jingrui , Chen Jie , Liang Xuefeng , Zhao Xiaomin , Zhou Chunhuo
Published: December 2024

The prevalent issue of cadmium (Cd) in naturally selenium (Se)-enriched soils has significantly impacted the safe utilization of Se-rich soils. Although Se antagonizes Cd and is affected by Se:Cd stoichiometry, the mechanism behind this interaction remains unclear. To reveal the relation between the soil bioavailable Se:Cd molar ratio (AMR-Se:Cd) and the transformation of Se and Cd fractions, as well as to identify the principal controlling factors, we conducted a study in Shanggao County, Yichun City, a naturally Se-rich area in Jiangxi Province, and quantitatively analyzed the distribution features of Se, Cd, and AMR-Se:Cd across different soil types and land use types. The results demonstrated that soil AMR-Se:Cd was statistically positively correlated with the bioavailable Se content (r = 0.331, p < 0.01) and had a negative correlation with the bioavailable Cd content (r = −0.402, p < 0.001). Cd was transformed from highly bioavailable fractions to less bioavailable fractions as the AMR-Se:Cd increased. A suitable AMR-Se:Cd was conducive to achieving a higher mobility of Se (mobility factor of 12.31%) and a lower mobility of Cd (mobility factor of 23.49%) simultaneously. Spearman correlation analysis and partial least squares path modeling revealed that soil type and land use type modulated the morphological transformation of soil Se and Cd by influencing changes in free Fe-Al oxides and soil organic matter content, which in turn altered the AMR-Se:Cd. Therefore, the findings of this study can offer guidance for regulating the appropriate AMR-Se:Cd in Se-rich soils through management practices to enhance the bioavailability and mobility of soil Se while diminishing the bioavailability and mobility of Cd.
Investigating the Effects of Dietary Bile Acids on Production Performance and Lipid Metabolism in Late-Phase Laying Hens
Authors: Wang Longfei , Fan Kefeng , Xing Ronghui , Yin Jixue , Si Xuemeng , Zhang Huaiyong , Huang Yanqun , Chen Wen
Published: December 2024

Multiply adverse effects including declines in production performance and excessive fat deposition were noticed with the extension of the laying cycle in hens, which are pertinent to animal welfare and human food safety. This study aimed to investigate the effect of dietary supplementation of bile acids (BAs) on production performance and lipid metabolism in late-phase laying hens. A total of 144 70-week-old hens were distributed into three treatments with eight replicates per treatment, including the basal diet with 0 (Ctrl), 95.01 (Low-BA), and 189.99 mg/kg (High-BA) of porcine BAs, respectively. The test period was from 70 to 75 weeks. The supplementation of BAs did not significantly alter laying performance during the trial, whereas it increased (p < 0.05) the total follicles compared to the Ctrl diet. The eggs from the hens fed the BA diet exhibited increased (p > 0.05) relative weight of eggshell and yolk color than those that consumed the Ctrl diet. There were no significant changes following BA treatment regarding the serum lipid profile. Dietary BA treatment reduced the total triglyceride in livers to different extents, resulting in the decreased diameter and area of vacuoles in liver tissues. The low-dose BA treatment decreased the mRNA levels of fatty acid synthase (FASN) and stearoyl-CoA desaturase (SCD), while promoting the expression of lipoprotein lipase (LPL) compared to the Ctrl group (both p < 0.05). Of note, the expressions of farnesoid X receptor (FXR), apical sodium-dependent bile acid transporter (ASBT), and ileum bile acid-binding protein (IBABP) were notably downregulated (p < 0.05) by the low-dose BA treatment. Dietary BA treatment had no apparent effects on laying performance, whereas it increased the follicle frequency, eggshell weight, and yolk color. Moreover, a diet containing 95.01 mg/kg of BAs depressed ileal BA resorption and hepatic fatty deposition by reducing lipogenesis and promoting lipolysis, which may have a beneficial effect on the liver in late-phase layers.
The Effects of a Blend of Essential Oils in the Milk of Suckling Calves on Performance, Immune and Antioxidant Systems, and Intestinal Microbiota
Authors: Nora Luisa , Marcon Charles , Deolindo Guilherme Luiz , Signor Mateus Henrique , Muniz Ana Luiza , Bajay Miklos Maximiliano , Copetti Priscila Marquezan , Bissacotti Bianca Fagan , Morsch Vera M. , da Silva Aleksandro Schafer
Published: December 2024

The objective of the present study was to determine whether the addition of a blend based on the essential oils of cinnamon, oregano, and eucalyptus to the liquid diets of calves would stimulate the immune system combined with anti-inflammatory action, minimize oxidative responses, and alter the intestinal microbiota, consequently enhancing animal growth. Twenty-four male Holstein calves (approximately five days old) were suckled for 60 days, underwent a weaning process, and were followed up until day 75 of the experiment. The calves were divided into control (n = 12) and phytobiotic (n = 12) groups, receiving commercial milk replacer and pelleted concentrate ad libitum. For the phytobiotic group, we added the blend to the liquid diet twice daily at 5 mL/feeding in the first 15 days and 10 mL/feeding until day 60. We detected no differences in weight gain, but animals in the phytobiotic group tended to consume less feed. Calves treated with phytobiotics showed better conversion and feed efficiency than the animals in the control group. Lower leukocyte and lymphocyte counts were observed, as was a higher cholesterol concentration. Immunoglobulin A, ceruloplasmin, and transferrin also differed between groups, with higher IgA and lower levels of acute-phase proteins (ceruloplasmin and transferrin) in calves that consumed the phytobiotic. Higher glutathione S-transferase activity was found in the serum of calves in the treatment group. The intestinal microbiota did not differ between the groups; however, the genera Acinetobacter, Pseudomonas, and Psychrobacter were the most abundant regardless of treatment. We concluded that the blend of oils based on cinnamon, oregano, and eucalyptus improved the calves’ immune and antioxidant systems, improving feed efficiency without affecting the intestinal microbiota.
Grey Wolf (Canis lupus) Recolonization in Hungary: Does the Predation Risk Affect the Red Deer (Cervus elaphus) Population?
Authors: Biró Zsolt , Katona Krisztián , Szabó László , Sütő Dávid , Heltai Miklós
Published: December 2024

The populations and distribution areas of large carnivores have declined all over the world due to extirpation and habitat alteration and degradation. However, the grey wolf (Canis lupus) has recovered in Europe in recent decades and has been reappearing in Hungary since the 1990s. Since the dominant prey of this carnivore is the red deer (Cervus elaphus) and the wild boar (Sus scrofa) in Central and Eastern Europe, we aimed to study the impact of wolves on local deer populations. Based on hunters’ opinions, we expected an increasing wolf presence and intense effects of wolves on the stress level and body condition of deer. First, we examined the occupied area by wolf in the North Hungarian Mountains. The distribution map was based on a questionnaire among the game managers. To measure the influence of the reappearing predator population on the red deer individuals, we estimated the body condition (kidney fat and bone marrow index) and stress hormone level of faecal samples. We compared them between the areas colonised by wolves and control sites in the mountains. We revealed an increased distribution area of wolves in the mountains since 2014. The stress hormone level was lower in the wolf-free sites in the case of faeces gathered from the ground, but there was similar amount of cortisol in the samples collected from the hunted animals. The body condition indices were not different between the two area types (average kidney fat index > 100% and almost 100% for the bone marrow fat content). Our results do not support a very intense recent impact of the wolf population on the body condition and stress level of red deer in Hungary.
Embryonic Thermal Programming and Dietary Baicalein Supplementation Post-Hatch: Effects on Broiler Adipose Tissue Deposition
Authors: Sulaiman Usman , Vaughan Reagan , Siegel Paul , Liu Dongmin , Gilbert Elizabeth , Cline Mark
Published: December 2024

Optimization of growth performance and fat metabolism in broilers are critical for meat quality and overall production efficiency. This experiment investigated the effects of dietary baicalein supplementation and embryonic heat conditioning (EHC) on the growth performance and adipose tissue metabolism of 10-day old broilers. Fertile eggs were divided into control and EHC groups, with EHC eggs exposed to intermittent heating (39.5 °C) from day 7 to day 16 of incubation. Hatched chicks were further divided into four groups: CC (control control), CT (control treatment with baicalein), EC (embryonic heat control), and ET (embryonic heat treatment with baicalein), and were fed ad libitum. On day 10 post-hatch, blood and adipose tissue samples were collected for analysis. C/EBPα mRNA was lower in the ET group compared to the EC group and higher in the CT group compared to the CC group. PPARγ and HSL mRNAs were elevated in both the ET and CT groups relative to their controls. Additionally, plasma non-esterified fatty acid (NEFA) levels were significantly higher in the CT group compared to the CC group. These results indicate that baicalein supplementation, particularly when combined with embryonic heat conditioning, can modulate fat metabolism and potentially improve the growth performance of broilers, thereby offering insights into strategies for enhancing poultry production.
Drivers of Seasonal Change of Avian Communities in Urban Parks and Cemeteries of Latin America
Authors: Leveau Lucas M. , Bocelli Lucia , Quesada-Acuña Sergio Gabriel , González-Lagos César , Tapia Pablo Gutierrez , Dri Gabriela Franzoi , Delgado-V Carlos A. , Garitano-Zavala Alvaro , Campos Jackeline , Benedetti Yanina , Ortega-Álvarez Rubén , Contreras-Rodríguez Anotnio Isain , Souza López Daniela , Fontana Carla Suertegaray , Silva Thaiane Weinert da , Zalewski Vargas Sarah S. , Toledo Maria C. B. , Sarquis Juan Andres , Giraudo Alejandro , Echevarria Ada Lilian , Fanjul María Elisa , Martínez María Valeria , Haedo Josefina , Gonzalo Cano Sanz Luis , Peña Dominguez Yuri A. , Fernandez-Maldonado Viviana , Marinero Veronica , Abilhoa Vinícius , Amorin Rafael , Escobar-Ibáñez Juan Fernando , Juri María Dolores , Camín Sergio R. , Marone Luis , Piratelli Augusto João , Franchin Alexandre G. , Crispim Larissa , Morelli Federico
Published: December 2024

Urban parks and cemeteries constitute hot spots of bird diversity in urban areas. However, the seasonal dynamics of their bird communities have been scarcely explored at large scales. This study aims to analyze the drivers of urban bird assemblage seasonality in urban parks and cemeteries comparing assemblages during breeding and non-breeding seasons in the Neotropical Region. Since cemeteries have less human disturbance than urban parks, we expected differences in bird community seasonality between habitats. The seasonal change of species composition was partitioned into species turnover and nestedness. At large scales, the seasonal change of species composition was positively related to temperature seasonality and was higher in the Northern Hemisphere. At the landscape scale, the seasonal change of composition decreased in sites located in the most urbanized areas. At the local scale, sites with the highest habitat diversity and pedestrian traffic had the lowest seasonal change of composition. The species turnover was higher in the Northern Hemisphere, augmented with increasing annual temperature range, and decreased in urban parks. The species nestedness was positively related to habitat diversity. Our results showed that a multi-scale framework is essential to understand the seasonal changes of bird communities. Moreover, the two components of seasonal composition dissimilarity showed contrasting responses to environmental variables. Although the surrounding urbanization lowered the seasonal dynamics of urban green areas, cemeteries seem to conserve more seasonal changes than urban parks. Thus, urban cemeteries help to conserve the temporal dynamics of bird communities in cities.
The Role of Pet-Based Activities: Working with Rabbits to Improve Self-Esteem in Preschool Children
Authors: Kim Sang-Hwan
Published: December 2024

Animal-assisted activities (AAAs) effectively improve the self-esteem of infants and young children, thereby playing a significant role in their growth into adolescence. In particular, in modern society, self-esteem during childhood and adolescence can influence self-esteem during adulthood. Therefore, this study evaluates whether animal-assisted activities could improve infants’ self-esteem. There were ten participants in this study, with six one-hour sessions conducted twice a week with six-year-old children. The study proceeded with one host, two assistant hosts, one animal manager, one observer, and two helper rabbits. A self-esteem test was used as the research tool. Before and after the program, the pre-and post-tests were conducted separately to confirm the effect. Animal-mediated therapy in the program resulted in reducing the average scores in certain sub-areas of self-esteem. Additionally, the average score demonstrated a statistically significant increase in other areas. This study’s results showed that play programs involving animal-assisted activities can positively improve the self-esteem of infants and toddlers who develop early social skills.
Antimicrobial Use in the Animal Sector in Japan from 2011 to 2022
Authors: Hosoi Yuta , Matsuda Mari , Kawanishi Michiko , Harada Saki , Kumakawa Mio , Sekiguchi Hideto , Asai Tetsuo , Sekiya Tatsuro
Published: December 2024

Background/Objectives: Evaluating antimicrobial use (AMU) is essential in the investigation and implementation of antimicrobial resistance (AMR) prevention measures. Here, we examined AMU using an index (mg/kg biomass) that considers the antimicrobial sales volume and livestock biomass in Japan from 2011 to 2022. Methods: Antimicrobial sales volumes were obtained from JVARM data, and biomass data were obtained from reliable statistics. Beef cattle, dairy cattle, pigs, broiler chickens, layer chickens, fish raised in freshwater, and fish raised in seawater were targeted in this study. Results: Tetracycline accounted for 39%, macrolides for 18%, penicillins for 12%, and sulfonamides for 11% of the sales in 2022. The peak antimicrobial sales volume was 847 tons in 2017, and then declined to 766 tons by 2022 with fluctuations in the interim. From the perspective of mg/kg biomass, AMU tended to increase in beef cattle, dairy cattle, and fish raised in seawater, while pigs, broilers, layers, and fish raised in freshwater showed a decreasing trend. In broilers, the decreasing trend that could not be confirmed by sales amount alone was detected using the newly established index. Conclusions: By calculating the mg/kg biomass, it became possible to create an interpretation that is different from that of the simple sales quantity data. We believe that this indicator is stable, transparent, and easily understandable for national monitoring.
Green Tea Catechins and Skin Health
Authors: Zheng Xin-Qiang , Zhang Xue-Han , Gao Han-Qing , Huang Lan-Ying , Ye Jing-Jing , Ye Jian-Hui , Lu Jian-Liang , Ma Shi-Cheng , Liang Yue-Rong
Published: December 2024

Green tea catechins (GTCs) are a group of bioactive polyphenolic compounds found in fresh tea leaves (Camellia sinensis (L.) O. Kuntze). They have garnered significant attention due to their diverse health benefits and potential therapeutic applications, including as antioxidant and sunscreen agents. Human skin serves as the primary barrier against various external aggressors, including pathogens, pollutants, and harmful ultraviolet radiation (UVR). Skin aging is a complex biological process influenced by intrinsic factors such as genetics and hormonal changes, as well as extrinsic factors like environmental stressors, among which UVR plays a pivotal role in accelerating skin aging and contributing to various dermatological conditions. Research has demonstrated that GTCs possess potent antioxidant properties that help neutralize free radicals generated by oxidative stress. This action not only mitigates cellular damage but also supports the repair mechanisms inherent in human skin. Furthermore, GTCs exhibit anti-carcinogenic effects by inhibiting pathways involved in tumor promotion and progression. GTCs have been shown to exert anti-inflammatory effects through modulation of inflammatory signaling pathways. Chronic inflammation is known to contribute significantly to both premature aging and various dermatological diseases such as psoriasis or eczema. By regulating these pathways effectively, GTCs may alleviate symptoms associated with inflammatory conditions. GTCs can enhance wound healing processes by stimulating angiogenesis. They also facilitate DNA repair mechanisms within dermal fibroblasts exposed to damaging agents. The photoprotective properties attributed to GTCs further underscore their relevance in skincare formulations aimed at preventing sun-induced damage. Their ability to screen UV light helps shield underlying tissues from harmful rays. This review paper aims to comprehensively examine the beneficial effects of GTCs on skin health through an analysis encompassing in vivo and in vitro studies alongside insights into molecular mechanisms underpinning these effects. Such knowledge could pave the way for the development of innovative strategies focused on harnessing natural compounds like GTCs for improved skincare solutions tailored to combat environmental stresses faced by the human epidermis.
Investigation of Oil Extracted from Roasted and Unroasted Oats with Use of Chemometrics
Authors: Palani Bharani Kumar , Siol Marta , Makouie Sina , Bryś Joanna , Gruczyńska-Sękowska Eliza , Koczoń Piotr
Published: December 2024

One of the beneficial components of oats is oil that is rich in fatty acids and has excellent health and technological potential. During thermal processing, the oil is prone to chemical changes, both beneficial and adverse. One such processing method is roasting, which involves heating the product uniformly at a temperature below its melting point. The primary objective is to evaluate and report with statistical models how the chemical changes that occur during the roasting of whole oat grains affect the properties and quality of the oil found in the oat grains. To achieve this goal, a pressure differential scanning calorimeter, infrared spectroscopy calorimetric bomb, and gas chromatography were used. Using chemometric methods, the spectral data were combined with calorimetric data, automatic titration data, and chromatographic data. The discriminant and reference models of high statistical significance were calibrated and validated to rapidly and robustly evaluate the properties of oat oil and the changes that occurred due to the roasting process. It has been shown that roasting oat grains increases the oxidative stability of the oil contained in grains. The acid and peroxide values of oil extracted from roasted oat grains are lower than those determined for oil from fresh oat grains. The composition of fatty acids was not statistically significantly affected by the roasting process of oat grains.
Research on GNSS/IMU/Visual Fusion Positioning Based on Adaptive Filtering
Authors: Liu Ao , Guo Hang , Yu Min , Xiong Jian , Liu Huiyang , Xie Pengfei
Published: December 2024

The accuracy of satellite positioning results depends on the number of available satellites in the sky. In complex environments such as urban canyons, the effectiveness of satellite positioning is often compromised. To enhance the positioning accuracy of low-cost sensors, this paper combines the visual odometer data output by Xtion with the GNSS/IMU integrated positioning data output by the satellite receiver and MEMS IMU both in the mobile phone through adaptive Kalman filtering to improve positioning accuracy. Studies conducted in different experimental scenarios have found that in unobstructed environments, the RMSE of GNSS/IMU/visual fusion positioning accuracy improves by 50.4% compared to satellite positioning and by 24.4% compared to GNSS/IMU integrated positioning. In obstructed environments, the RMSE of GNSS/IMU/visual fusion positioning accuracy improves by 57.8% compared to satellite positioning and by 36.8% compared to GNSS/IMU integrated positioning.
Moulded-Pulp Packaging: A Straightforward Method for Quickly Designing, Manufacturing and Testing Complex Shapes for Crash Protection Pads
Authors: Humbert Claude , Jadeau-Guichard Hector , Nicolay Pascal
Published: December 2024

Moulded-pulp packaging stands poised to replace traditional polystyrene packaging due to its eco-friendly nature and recyclability. However, optimizing the protective pins within moulded-pulp packaging has been a challenge, hindering its widespread adoption. This paper presents an innovative approach to swiftly and cost-effectively optimize pin shapes, which is crucial for enhancing impact resistance. We hereby propose a simple try and error pin optimization methodology by utilizing an hand-moulded pulp recipe and PLA 3D-printed moulds to rapidly craft tailored cardboard pins. The validation of the material fabrication was carried out with static characterization and the classification of the homemade moulded-pulp pins with impact testing analysis. This exploratory project demonstrates the feasibility of this approach, laying the foundation for future advancements in moulded-pulp packaging design.