bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Temperature and pH on Recombinant Thaumatin II Production by Pichia pastoris
Authors: Joseph Jewel Ann , Akkermans Simen , Van Impe Jan F. M.
Published: May 2022

The sweet protein thaumatin is emerging as a promising sugar replacer in the market today, especially in the food and beverage sector. Rising demand for its production necessitates the large-scale extraction of this protein from its natural plant source, which can be limited in terms of raw material availability and production costs. Using a recombinant production technique via a yeast platform, specifically, Pichia pastoris, is more promising to achieve the product economically while maintaining batch-to-batch consistency. However, the bioproduction of recombinant proteins requires the identification of optimal process variables, constituting the maximal yield of the product of interest. These variables have a direct effect on the growth of the host organism and the secretion levels of the recombinant protein. In this study, two important environmental factors, pH, and temperature were assessed by cultivating P. pastoris in shake flasks to understand their influence on growth and the production levels of thaumatin II protein. The results from the pH study indicate that P. pastoris attained a higher viable cell density and secretion of protein at pH 6.0 compared to 5.0 when grown at 30 °C. Furthermore, within the three levels of temperatures investigated when grown at pH 6.0, the protein levels were the highest at 30 °C compared to 20 and 25 °C, whereas 25 °C exhibited the highest viable cell density. Interestingly, the trend observed from the qualitative effects of temperature and pH occurred in all the media that was investigated. These results broaden our understanding of how pH and temperature adjustment during P. pastoris cultivation aid in enhancing the production yields of thaumatin II prior to optimising the fed batch bioreactor operation.
Consumption of Tritordeum Bread Reduces Immunogenic Gluten Intake without Altering the Gut Microbiota
Authors: Haro Carmen , Guzmán-López María H. , Marín-Sanz Miriam , Sánchez-León Susana , Vaquero Luis , Pastor Jorge , Comino Isabel , Sousa Carolina , Vivas Santiago , Landa Blanca B. , Barro Francisco
Published: May 2022

Gluten proteins are responsible for the wheat breadmaking quality. However, gluten is also related to human pathologies for which the only treatment is a gluten-free diet (GFD). GFD has gained popularity among individuals who want to reduce their gluten intake. Tritordeum is a cereal species that originated after crossing durum wheat with wild barley and differs from bread wheat in its gluten composition. In this work, we have characterized the immunogenic epitopes of tritordeum bread and results from a four-phase study with healthy adults for preferences of bread and alterations in the gut microbiota after consuming wheat bread, gluten-free bread, and tritordeum bread are reported. Tritordeum presented fewer peptides related to gluten proteins, CD-epitopes, and IgE binding sites than bread wheat. Participants rated tritordeum bread higher than gluten-free bread. Gut microbiota analysis revealed that the adherence to a strict GFD involves some minor changes, especially altering the species producing short-chain fatty acids. However, the short-term consumption of tritordeum bread does not induce significant changes in the diversity or community composition of the intestinal microbiota in healthy individuals. Therefore, tritordeum bread could be an alternative for healthy individuals without wheat-related pathologies who want to reduce their gluten consumption without harming their gut health.
Analysis of the Fruit Quality of Pear (Pyrus spp.) Using Widely Targeted Metabolomics
Authors: Zheng Pufan , Zhang Mei , Fang Xin , Tang Lili , Wang Zhixue , Shi Fuchen
Published: May 2022

Pear is a kind of common temperate fruit, whose metabolite composition that contributes to the difference in fruit quality is unclear. This study identified and quantified the metabolites using a widely targeted LC-MS/MS approach in three pear species, including Pyrus bretschneideri (PB), Pyrus usssuriensis (PU) and Pyrus pyrifolia (PP). A total of 493 metabolites were identified, consisting of 68 carbohydrates, 47 organic acids, 50 polyphenols, 21 amino acids, 20 vitamins, etc. The results of PCA and OPLS-DA demonstrated that the metabolite compositions differed distinctly with cultivar variability. Our results also involved some metabolic pathways that may link to the fruit quality based on KEGG pathway analysis, the pathway of phenylalanine metabolism revealed significant differences between PB and PP (p < 0.05). Furthermore, the study selected D-xylose, formononetin, procyanidin A1 and β-nicotinamide mononucleotide as the major differentially expressed metabolites in the three species. The present study can open new avenues for explaining the differences in fruit quality of the major commercial pear cultivars in China.
Knee Injury and Osteoarthritis Outcome Score Patellofemoral Questionnaire: Psychometric Properties among Females of Kingdom of Saudi Arabia
Authors: Alzhrani Msaad
Published: May 2022

Patellofemoral joint osteoarthritis (PFJ-OA), being a subset of knee osteoarthritis (KOA), is evident in adults, and its prevalence is greater in women in Saudi Arabia too. To assess its disease dimensions, the ‘Knee Injury and Osteoarthritis Outcome Score Patellofemoral’ questionnaire (KOOS-PF) is frequently used to measure symptoms and function among the people with PFJ-OA. Cross-cultural validation is ongoing in several languages, and it needed to be validated among females in Arabic. Therefore, aiming to translate, cross-culturally adapt and validate its psychometric properties, a cross-sectional study was designed where the Ar-KOOS-PF-F was administered among 105 females. The demographic characteristics of recruited females were 51.62 (8.49) years and 30.12 (3.70) kg/m2. Cronbach’s alpha was used for internal consistency (IC) and the questionnaire was re-administered after 48 h to estimate the test–retest reliability (92 females, 87.61% compliance rate). Concurrent validity was also established with a visual analog scale (VAS). Factorial validity was established by principal component analysis (PCA). The psychometric properties were: excellent internal consistency of Cronbach’s alpha (α) = 0.930, intraclass correlation coefficient (ICC) for intra-ratter reliability = 0.960 (0.915–0.999), test–retest reliability, ICC = 0.893 (0.889–0.970), standard error of measurement (SEM) = 2.46, relative standard deviation/coefficient of variance (RSD/CV) = 29.9%, minimal detectable change (MDC%) = 22.96% and good concurrent validity with VAS (r = −0.783; p = 0.023). The best-fit four-factor model for confirming overall item communalities ranged from 0.529 to 0.867, which indicates moderate to high communalities, and confirms the homogeneity of Ar-KOOS-PF-F using PCA. The floor (0.9%) and ceiling effects (13.6%) were also within the limits. This scale can be used among females, as it has acceptable psychometric properties of scale validation.
A Study of Financial Literacy of Investors—A Bibliometric Analysis
Authors: Ansari Yasmeen , Albarrak Mansour Saleh , Sherfudeen Noorjahan , Aman Arfia
Published: May 2022

The present study investigates a conceptual research framework on financial literacy in various investment planning and decision-making stages. The study comprises a review of 2182 articles published in peer-reviewed journals from 2001 to 2022 (January). The study employed bibliometric techniques such as citation network analysis, co-citation analysis, content analysis, publication trends, and keyword analysis to analyze the literature on financial literacy. The study aims to add to the literature on financial literacy by proposing ten clusters to improve research on financial literacy in order to help investors learn better. Financial literacy has evolved from a fledgling discipline to a significant teaching and research tool. Therefore, it is vital to investigate and identify current research trends in this field. The results are essential to the financial community, given that institutions and society are increasingly emphasizing financial literacy to strengthen individual citizens’ responsibilities in designing their investment strategies.
Integration of Eukaryotic Energy Metabolism: The Intramitochondrial and Cytosolic Energy States ([ATP]f/[ADP]f[Pi])
Authors: Wilson David F. , Matschinsky Franz M.
Published: May 2022

Maintaining a robust, stable source of energy for doing chemical and physical work is essential to all living organisms. In eukaryotes, metabolic energy (ATP) production and consumption occurs in two separate compartments, the mitochondrial matrix and the cytosol. As a result, understanding eukaryotic metabolism requires knowledge of energy metabolism in each compartment and how metabolism in the two compartments is coordinated. Central to energy metabolism is the adenylate energy state ([ATP]/[ADP][Pi]). ATP is synthesized by oxidative phosphorylation (mitochondrial matrix) and glycolysis (cytosol) and each compartment provides the energy to do physical work and to drive energetically unfavorable chemical syntheses. The energy state in the cytoplasmic compartment has been established by analysis of near equilibrium metabolic reactions localized in that compartment. In the present paper, analysis is presented for energy-dependent reactions localized in the mitochondrial matrix using data obtained from both isolated mitochondria and intact tissues. It is concluded that the energy state ([ATP]f/[ADP]f[Pi]) in the mitochondrial matrix, calculated from the free (unbound) concentrations, is not different from the energy state in the cytoplasm. Corollaries are: (1) ADP in both the cytosol and matrix is selectively bound and the free concentrations are much lower than the total measured concentrations; and (2) under physiological conditions, the adenylate energy states in the mitochondrial matrix and cytoplasm are not substantially different.
Genome-Wide Identification of Gramineae Brassinosteroid-Related Genes and Their Roles in Plant Architecture and Salt Stress Adaptation
Authors: Huang Jinyong , Ma Shengjie , Zhang Kaiyan , Liu Xiaohan , Hu Linlin , Wang Wenming , Zheng Liwei
Published: May 2022

Brassinosteroid-related genes are involved in regulating plant growth and stress responses. However, systematic analysis is limited to Gramineae species, and their roles in plant architecture and salt stress remain unclear. In this study, we identified brassinosteroid-related genes in wheat, barley, maize, and sorghum and investigated their evolutionary relationships, conserved domains, transmembrane topologies, promoter sequences, syntenic relationships, and gene/protein structures. Gene and genome duplications led to considerable differences in gene numbers. Specific domains were revealed in several genes (i.e., HvSPY, HvSMOS1, and ZmLIC), indicating diverse functions. Protein-protein interactions suggested their synergistic functions. Their expression profiles were investigated in wheat and maize, which indicated involvement in adaptation to stress and regulation of plant architecture. Several candidate genes for plant architecture (ZmBZR1 and TaGSK1/2/3/4-3D) and salinity resistance (TaMADS22/47/55-4B, TaGRAS19-4B, and TaBRD1-2A.1) were identified. This study is the first to comprehensively investigate brassinosteroid-related plant architecture genes in four Gramineae species and should help elucidate the biological roles of brassinosteroid-related genes in crops.
Delayed Recovery in Idiopathic Sudden Sensorineural Hearing Loss
Authors: Na Gina , Kim Ki-Won , Jung Keun-Woo , Yun Jimin , Cheong Taek-Yoon , Lee Jeon-Mi
Published: May 2022

Sudden hearing loss is an easily encountered disease in clinics, but its prognosis has not been completely elucidated. In the present study, we investigated the long-term prognosis of sudden hearing loss with 130 patients who were diagnosed based on strict criteria and provided uniform treatment. The patients with incomplete recovery were reevaluated after 2 months without receiving additional treatment. Hearing levels at different time points were compared. Moreover, the associated factors affecting the degree of hearing improvement over time were evaluated using stepwise multiple linear regression. After treatment, 73 out of the 130 (56.1%) patients attained incomplete recovery and were reevaluated after 2 months. Seventeen out of the seventy-three (23.3%) patients showed a grade promotion, fifty-four (74%) were constant, and two (2.7%) were aggravated. The mean interaural hearing differences (IHDs) showed significant improvement. Old age, poor initial IHD, and poor recovery grade were significantly associated with a profitable delayed hearing gain. Poorer hearing level at the time of onset might be a sign for slower recovery rather than a poorer prognostic factor. The treatment outcome of idiopathic sudden sensorineural hearing loss (ISSNHL) should be evaluated at least 2 months after treatment completion, and counseling is required due to the need for long-term follow-up in patients with ISSNHL.
Simplified TAVR Procedure: How Far Is It Possible to Go?
Authors: Leclercq Florence , Meunier Pierre Alain , Gandet Thomas , Macia Jean-Christophe , Delseny Delphine , Gaudard Philippe , Mourad Marc , Schmutz Laurent , Robert Pierre , Roubille François , Cayla Guillaume , Akodad Mariama
Published: May 2022

Increasing operators’ experience and improvement of the technique have resulted in a drastic reduction in complications following transcatheter aortic valve replacement (TAVR) in patients with lower surgical risk. In parallel, the procedure was considerably simplified, with a routine default approach including local anesthesia in the catheterization laboratory, percutaneous femoral approach, radial artery as the secondary access, prosthesis implantation without predilatation, left ventricle wire pacing and early discharge. Thus, the “simplified” TAVR adopted in most centers nowadays is a real revolution of the technique. However, simplified TAVR must be accompanied upstream by a rigorous selection of patients who can benefit from a minimalist procedure in order to guarantee its safety. The minimalist strategy must not become dogmatic and careful pre-, per- and post-procedural evaluation of patients with well-defined protocols guarantee optimal care following TAVR. This review aims to evaluate the benefits and limits of the simplified TAVR procedure in a current and future vision.
Design and Rationale of the Sevoflurane for Sedation in Acute Respiratory Distress Syndrome (SESAR) Randomized Controlled Trial
Authors: Blondonnet Raiko , Simand Laure-Anne , Vidal Perine , Borao Lucile , Bourguignon Nathalie , Morand Dominique , Bernard Lise , Roszyk Laurence , Audard Jules , Godet Thomas , Monsel Antoine , Garnier Marc , Quesnel Christophe , Bazin Jean-Etienne , Sapin Vincent , Bastarache Julie A. , Ware Lorraine B. , Hughes Christopher G. , Pandharipande Pratik P. , Ely E. Wesley , Futier Emmanuel , Pereira Bruno , Constantin Jean-Michel , Jabaudon Matthieu ,   on behalf of the SESAR Collaborative Group
Published: May 2022

Preclinical studies have shown that volatile anesthetics may have beneficial effects on injured lungs, and pilot clinical data support improved arterial oxygenation, attenuated inflammation, and decreased lung epithelial injury in patients with acute respiratory distress syndrome (ARDS) receiving inhaled sevoflurane compared to intravenous midazolam. Whether sevoflurane is effective in improving clinical outcomes among patients with ARDS is unknown, and the benefits and risks of inhaled sedation in ARDS require further evaluation. Here, we describe the SESAR (Sevoflurane for Sedation in ARDS) trial designed to address this question. SESAR is a two-arm, investigator-initiated, multicenter, prospective, randomized, stratified, parallel-group clinical trial with blinded outcome assessment designed to test the efficacy of sedation with sevoflurane compared to intravenous propofol in patients with moderate to severe ARDS. The primary outcome is the number of days alive and off the ventilator at 28 days, considering death as a competing event, and the key secondary outcome is 90 day survival. The planned enrollment is 700 adult participants at 37 French academic and non-academic centers. Safety and long-term outcomes will be evaluated, and biomarker measurements will help better understand mechanisms of action. The trial is funded by the French Ministry of Health, the European Society of Anaesthesiology, and Sedana Medical.
Impact of Cardiac Troponin Elevation on Mortality of Patients with Acute Heart Failure: Insights from the Korea Acute Heart Failure (KorAHF) Registry
Authors: Lee Nuri , Cho Jae Yeong , Kim Kye Hun , Kim Hyung Yoon , Cho Hyun-Jai , Lee Hae-Young , Jeon Eun-Seok , Kim Jae-Joong , Cho Myeong-Chan , Chae Shung Chull , Baek Sang Hong , Kang Seok-Min , Choi Dong-Ju , Yoo Byung-Su , Oh Byung-Hee ,   on behalf of the KorAHF Investigators
Published: May 2022

We aimed to conduct the largest study evaluating the impact of cardiac troponin (TnI) status on mid- and long-term mortality in patients admitted for acute heart failure (AHF) as compared between patients with ischemic (IHF) vs. non-ischemic heart failure (non-IHF). Among 5625 patients from the Korea Acute Heart Failure (KorAHF) registry, 4396 eligible patients with TnI measurement were analyzed. The patients were included on admission with the diagnosis of AHF, and TnI level was measured on the day of admission. A TnI value of <0.05 ng/mL was considered normal. The patients were divided into four groups according to the etiology of heart failure and the status of TnI: non-IHF with normal TnI (n = 1009) vs. non-IHF with elevated TnI (n = 1665) vs. IHF with normal TnI (n = 258) vs. IHF with elevated TnI (n = 1464). The primary outcome was death from all causes according to the etiology (non-IHF vs. IHF) and TnI elevation during the entire follow-up period of 784 days (IQR 446–1116). Elevation of TnI was observed in 71.2% of all patients with AHF. Patients with IHF had higher all-cause mortality compared to those with non-IHF. Elevated TnI was associated with higher 90-day and post-90-day mortality in the non-IHF group. IHF as compared to non-IHF and elevation of TnI were independent predictors of mortality also in the adjustment analysis. In the IHF group, however, elevated TnI had a higher mortality with only 90-day follow-up (18.6% vs. 25.9%, log-rank p < 0.001), not in the post-90-day follow-up (31.1% vs. 32.5%, log-rank p = 0.799). In conclusion, elevated TnI in patients with heart failure is associated with increased all-cause mortality regardless of the etiology of HF. Elevation of TnI was associated to a higher post-90 day mortality in patients with non-IHF but not in patients with IHF.
Sharing Patient and Clinician Experiences of Moderate-to-Severe Psoriasis: A Nationwide Italian Survey and Expert Opinion to Explore Barriers Impacting upon Patient Wellbeing
Authors: Prignano Francesca , Brunasso Alexandra M. G. , Fabbrocini Gabriella , Argenziano Giuseppe , Bardazzi Federico , Borroni Riccardo G. , Burlando Martina , Cagni Anna Elisabetta , Campione Elena , Cinotti Elisa , Cuccia Aldo , Dastoli Stefano , De Pasquale Rocco , De Simone Clara , Di Lernia Vito , Dini Valentina , Fargnoli Maria Concetta , Faure Elisa , Giacchetti Alfredo , Giofrè Claudia , Girolomoni Giampiero , Lasagni Claudia , Lembo Serena , Loconsole Francesco , Montesu Maria Antonia , Pella Paolo , Pigatto Paolo , Richetta Antonio Giovanni , Stroppiana Elena , Venturini Marina , Zichichi Leonardo , Piaserico Stefano
Published: May 2022

A nationwide survey was conducted in adult patients with psoriasis (PsO) across Italy to obtain their real-world perspective of the impact of PsO on their wellbeing. Patients completed a 26-question survey (based on the patient benefit index; PBI, The Dermatology Life Quality Index; DLQI and the World Health Organization-five; WHO-5 wellbeing index) and workshop discussion sessions were undertaken by dermatologists to interpret results from the survey. 392 patients with PsO completed the survey. Analysis of results was restricted to patients who had moderate-to-severe plaque psoriasis (assessed by patients; n = 252; 64.3%). Dermatologists (n = 32) completed one question from the survey related to wellbeing and rated social, physical and mental domains as contributing to a similar extent, with comparable scores also observed by patients. For treatment, biologics yielded higher scores on average, whereas little difference was observed between topical and conventional systemic treatments. Only 23.8% of patients felt that their dermatologist was taking into consideration their wellbeing and 32.6% of the patients considered their therapy as inadequate in improving signs and symptoms of the disease. This survey identified key factors contributing to barriers impacting on patient wellbeing. Simple, but comprehensive questionnaires can provide important insight to patients’ needs that may significantly increase clinician awareness during visits leading to tailored treatment.
Early Cellular and Humoral Responses Developed in Oncohematological Patients after Vaccination with One Dose against COVID-19
Authors: Rodríguez-Mora Sara , Corona Magdalena , Torres Montserrat , Casado-Fernández Guiomar , García-Pérez Javier , Ramos-Martín Fernando , Vigón Lorena , Manzanares Mario , Mateos Elena , Martín-Moro Fernando , Zurdo-Castronuño Alejandro , Murciano-Antón María Aranzazu , Alcamí José , Pérez-Olmeda Mayte , López-Jiménez Javier , García-Gutiérrez Valentín , Coiras Mayte ,   on behalf of the Multidisciplinary Group of Study of COVID-19 (MGS-COVID)
Published: May 2022

Individuals with oncohematological diseases (OHD) may develop an impaired immune response against vaccines due to the characteristics of the disease or to its treatment. Humoral response against SARS-CoV-2 has been described to be suboptimal in these patients, but the quality and efficiency of the cellular immune response has not been yet completely characterized. In this study, we analyzed the early humoral and cellular immune responses in individuals with different OHD after receiving one dose of an authorized vaccine against SARS-CoV-2. Humoral response, determined by antibodies titers and neutralizing capacity, was overall impaired in individuals with OHD, except for the cohort of chronic myeloid leukemia (CML), which showed higher levels of specific IgGs than healthy donors. Conversely, the specific direct cytotoxic cellular immunity response (DCC) against SARS-CoV-2, appeared to be enhanced, especially in individuals with CML and chronic lymphocytic leukemia (CLL). This increased cellular immune response, developed earlier than in healthy donors, showed a modest cytotoxic activity that was compensated by significantly increased numbers, likely due to the disease or its treatment. The analysis of the immune response through subsequent vaccine doses will help establish the real efficacy of COVID-19 vaccines in individuals with OHD.
Bruton’s Kinase Inhibitors for the Treatment of Immunological Diseases: Current Status and Perspectives
Authors: Robak Ewa , Robak Tadeusz
Published: May 2022

The use of Bruton’s tyrosine kinase (BTK) inhibitors has changed the management of patients with B-cell lymphoid malignancies. BTK is an important molecule that interconnects B-cell antigen receptor (BCR) signaling. BTK inhibitors (BTKis) are classified into three categories, namely covalent irreversible inhibitors, covalent reversible inhibitors, and non-covalent reversible inhibitors. Ibrutinib is the first covalent, irreversible BTK inhibitor approved in 2013 as a breakthrough therapy for chronic lymphocytic leukemia patients. Subsequently, two other covalent, irreversible, second-generation BTKis, acalabrutinib and zanubrutinib, have been developed for lymphoid malignancies to reduce the ibrutinib-mediated adverse effects. More recently, irreversible and reversible BTKis have been under development for immune-mediated diseases, including autoimmune hemolytic anemia, immune thrombocytopenia, multiple sclerosis, pemphigus vulgaris, atopic dermatitis, rheumatoid arthritis, systemic lupus erythematosus, Sjögren’s disease, and chronic spontaneous urticaria, among others. This review article summarizes the preclinical and clinical evidence supporting the role of BTKis in various autoimmune, allergic, and inflammatory conditions.
Correlation between Electrodiagnostic Study and Imaging Features in Patients with Suspected Carpal Tunnel Syndrome
Authors: Song Jae Min , Kim Jungyun , Chae Dong-Jin , Park Jong Bum , Lee Yung Jin , Hwang Cheol Mog , Shin Jieun , Hong Mi Jin
Published: May 2022

Electrodiagnostic studies (EDXs) are the confirmative diagnostic tool for carpal tunnel syndrome (CTS). Previous studies have evaluated the relationship between EDXs and ultrasonography (US) but not with X-rays. Recently, many studies on the diagnostic value of X-rays in various diseases have been reported, but data on CTS are lacking. We evaluated the relationship between electrodiagnostic parameters and roentgenographic and ultrasonographic features in CTS and investigated the usefulness of X-rays and US for CTS. This retrospective study included 97 wrists of 62 patients. All patients with suspected CTS underwent EDXs, wrist US, and wrist X-rays. The CTS patients were classified into mild, moderate, and severe groups. The roentgenographic features included the ulnar variance (UV) and the anteroposterior diameter of the wrist (APDW), and the ultrasonographic features included the flattening ratio (FR) and the thickest anteroposterior diameter of the median nerve (TAPDM). Most EDX parameters showed significant correlations with roentgenographic and US features. The electrodiagnostic severity was also correlated with all imaging features. Therefore, both wrist X-rays and wrist US can be useful for the diagnosis of CTS as supplements to EDXs.
Sjögren’s Syndrome Associated Dry Eye: Impact on Daily Living and Adherence to Therapy
Authors: Michaelov Evan , McKenna Caroline , Ibrahim Pierre , Nayeni Manav , Dang Arpit , Mather Rookaya
Published: May 2022

Sjögren’s syndrome-related dry eye disease (SS-DED) often involves more severe dry eye symptoms than people with non-SS dry eye disease (DED). This cross-sectional study employed an anonymous self-administered questionnaire to understand the experience of people living with SS-DED and to identify factors affecting adherence to DED self-care. Participants reported difficulty with visual tasks such as driving, and diminished enjoyment in daily activities due to DED symptoms. Almost 80% reported being worried about a reduced quality of life due to DED, and over 50% reported fear of blindness. The most common reasons for non-adherence were cost of therapy and forgetting to instill drops. Drop rationing to reduce cost of therapy was endorsed by 83% of respondents. Only 3% of respondents had private insurance for non-prescription agents required to treat DED. A quarter of respondents reported they would not disclose non-adherence to their eye care provider. Multiple regression analysis revealed age was a significant contributor to missing drops. This is the first study to report on the financial burden experienced by SS-DED patients in Canada. This paper identified strategies used by patients to reduce the cost of therapy and its impact on adherence to treatment. Patients may be reluctant to disclose challenges regarding adherence to DED therapy, as well as fears of worsening quality of life.
Drug-Related Hypersensitivity Reactions Leading to Emergency Department: Original Data and Systematic Review
Authors: Pagani Silvia , Lombardi Niccolò , Crescioli Giada , Vighi Violetta Giuditta , Spada Giulia , Andreetta Paola , Capuano Annalisa , Vannacci Alfredo , Venegoni Mauro , Vighi Giuseppe Danilo ,   on behalf of the MEREAFaPS Study Group
Published: May 2022

The aim of the present study is to describe pharmacological characteristics of drug-related allergies and anaphylaxis leading to the emergency department (ED). An 8-year post hoc analysis on the MEREAFaPS Study database was performed (2012–2019). Subjects who experienced drug-related hypersensitivity leading to an ED visit were selected. Logistic regression analyses were used to estimate the reporting odds ratios (RORs) of drug-related allergies and anaphylaxis adjusting for sex, age classes, and ethnicity. In addition, a systematic review of observational studies evaluating drug-related hypersensitivity reactions leading to ED visits in outpatients was performed. Out of 94,073 ED visits, 14.4% cases were drug-related allergies and 0.6% were anaphylaxis. Females accounted for 56%. Multivariate logistic regression showed a higher risk of drug-related allergy among males and all age classes < 65 years, while a higher risk of anaphylaxis was observed for females (ROR 1.20 [1.01–1.42]) and adults (ROR 2.63 [2.21–3.14]). The systematic review included 37 studies. ED visits related to allergy and anaphylaxis ranged from 0.004% to 88%, and drug-related allergies and anaphylaxis ranged from 0.007% to 88%. Both in our analysis and in primary studies, antibacterials, analgesics, and radiocontrast agents were identified as the most common triggers of hypersensitivity.
Cardiovascular Profile of South African Adults with Low-Level Viremia during Antiretroviral Therapy
Authors: Botha-Le Roux Shani , Elvstam Olof , De Boever Patrick , Goswami Nandu , Magnusson Martin , Nilsson Peter , Strijdom Hans , Björkman Per , Fourie Carla
Published: May 2022

Chronic inflammation is an HIV infection feature, contributing to elevated risk of cardiovascular disease among people with HIV, which can be induced by viral replication. A proportion of antiretroviral therapy (ART) recipients fail to achieve viral suppression, despite not meeting criteria for treatment failure, so-called low-level viremia (LLV). We investigated the relationship between LLV and an array of cardiovascular measures and biomarkers. South Africans with LLV (viral load = 50–999 copies/mL) and virological suppression (viral load <50 copies/mL) were selected from the EndoAfrica study (all receiving efavirenz-based ART) for cross-sectional comparison of vascular structure and function measures, as well as 21 plasma biomarkers related to cardiovascular risk and inflammation. Associations were investigated with univariate, multivariate, and binomial logistic regression analyses (having outcome measures above (cases) or below (controls) the 75th percentile). Among 208 participants, 95 (46%) had LLV, and 113 (54%) had viral suppression. The median age was 44 years, 73% were women, and the median ART duration was 4.5 years. Cardiovascular measures and biomarker levels were similar between these two categories. Cardiovascular function and structure measures were not associated with viremia status and having LLV did not increase the odds of having outcome measures above the 75th percentile. In this study among South African ART recipients, LLV did not associate with cardiovascular risk.
Experimental and Numerical Study of the Influence of Clumped Weights on a Scaled Mooring Line
Authors: Lopez-Olocco Tomas , González-Gutiérrez Leo M. , Calderon-Sanchez Javier , Marón Loureiro Adolfo , Saavedra Ynocente Leandro , Bezunartea Barrio Ana , Vivar Valdés Nicolás
Published: May 2022

Recently, several experimental and numerical studies have underlined the advantages of adding clumped weights at discrete positions of mooring lines. To confirm the influence of these weights, an experimental study was performed for a 1:30 scale model of a mooring line. In this study, the clumped weight is modeled as a scaled disc placed at different positions along the mooring line. The series of experiments has been carried out at the CEHIPAR towing tank using a submerged studless chain both with and without clumped weights. The experiments consist of the excitation of the suspension point with horizontal periodic motions using different amplitudes and periods, where the mooring line’s tension at the fairlead is measured using a load cell and a dynamometer, and the motion of a part of the line is recorded using low-cost submerged cameras. Similarly to previous experiments, the fairlead tensions increase with higher amplitudes and lower periods, and a clear pattern in the motions of the line at different depths is found. The dissipated energy and the fairlead tension is also increased by the addition of the clumped weight, and the variation of this energy with its position along the line is monitored. The presence of clumped weights is also implemented into a finite element numerical code, previously validated without clumped weights, where all the previous experiments with clumped weights are replicated with remarkable accuracy. This double experimental and computational approach to the problem provides an important dataset for numerical code validations and opens future discussions about the impact of clumped weights on floating platforms.
Effect of Lateral Stress on the Mechanical Properties of Rock Fracture and Its Implication on the Stability of Underground Oil Storage Caverns
Authors: Qiao Liping , Wang Zhechao , Li Wei , Liu Huan , Yang Lei
Published: May 2022

It is of great significance to study the mechanical properties of rock fracture for the stability of rock engineering projects. The mechanical properties, including the normal compression and shear properties of rock fracture, are studied by a series of shear tests considering the effect of lateral stress using the self-developed true triaxial test apparatus. The test results show that the initial normal stiffness and the maximum normal closure value of rock fracture increase with the increase of lateral stress, and the peak shear strength and the peak dilatancy angle increase with the increase of lateral stress, whereas the peak shear displacement decreases with the increase of lateral stress. Considering the effect of lateral stress, the improved normal loading model, peak shear strength model and peak dilatancy angle model of rock fracture are established. Using the equivalent parameters of rock fracture obtained based on the test, the hydro-mechanical coupling analysis considering lateral stress is carried out for an underground water sealed oil storage cavern project. It can be concluded that with the increase of lateral stress, the displacement of surrounding rock decreases, and the surrounding rock tends to be more stable.
Comment on Rosenzweig et al. Very Low Prostate PET/CT PSMA Uptake May Be Misleading in Staging Radical Prostatectomy Candidates. J. Pers. Med. 2022, 12, 410
Authors: Veerman Hans , Vis André N. , Donswijk Maarten , van der Poel Henk G.
Published: May 2022

With interest, we read the article by Rosenzweig et al. [...]
Hormonal Regulation of Renal Fibrosis
Authors: Abramicheva Polina , Plotnikov Egor
Published: May 2022

Fibrosis is a severe complication of many acute and chronic kidney pathologies. According to current concepts, an imbalance in the synthesis and degradation of the extracellular matrix by fibroblasts is considered the key cause of the induction and progression of fibrosis. Nevertheless, inflammation associated with the damage of tissue cells is among the factors promoting this pathological process. Most of the mechanisms accompanying fibrosis development are controlled by various hormones, which makes humoral regulation an attractive target for therapeutic intervention. In this vein, it is particularly interesting that the kidney is the source of many hormones, while other hormones regulate renal functions. The normal kidney physiology and pathogenesis of many kidney diseases are sex-dependent and thus modulated by sex hormones. Therefore, when choosing therapy, it is necessary to focus on the sex-associated characteristics of kidney functioning. In this review, we considered renal fibrosis from the point of view of vasoactive and reproductive hormone imbalance. The hormonal therapy possibilities for the treatment or prevention of kidney fibrosis are also discussed.
Diagnostic and Therapeutic Approach to Children and Adolescents with Obstructive Sleep Apnea Syndrome (OSA): Recommendations in Emilia-Romagna Region, Italy
Authors: Esposito Susanna , Ricci Giampiero , Gobbi Riccardo , Vicini Claudio , Caramelli Fabio , Pizzi Silvia , Fadda Agatina , Ferro Salvatore , Plazzi Giuseppe
Published: May 2022

Obstructive sleep apnoea syndrome (OSA) in paediatrics is a rather frequent pathology caused by pathophysiological alterations leading to partial and prolonged obstruction (hypoventilation) and/or intermittent partial (hypopnoea) or complete (apnoea) obstruction of the upper airways. Paediatric OSA is characterised by daytime and night-time symptoms. Unfortunately, there are few data on shared diagnostic-therapeutic pathways that address OSA with a multidisciplinary approach in paediatric age. This document summarizes recommendations from the Emilia-Romagna Region, Italy, developed in order to provide the most appropriate tools for a multidisciplinary approach in the diagnosis, treatment and care of paediatric patients with OSA. The multidisciplinary group of experts distinguished two different ‘step’ pathways, depending on the age group considered (i.e., under or over two years). In most cases, these pathways can be carried out by the primary care paediatrician, who represents the first filter for approaching the problem. For this reason, it is essential that the primary care paediatrician receives adequate training on how to formulate the diagnostic suspicion of OSA and on what criteria to use to select patients to be sent to the hospital centre. The relationship between the paediatrician of the patient and her/his parents must see a synergy of behaviour between the various players in order to avoid uncertainty about the diagnostic and therapeutic decisions as well as the follow-up phase. The definition and evaluation of the organizational process and outcome indicators of the developed flow-chart, and the impact of its implementation will remain fundamental.
Static Friction in a Cable-Driven Transmission—Modeling and Identification of Load Effects
Authors: Torres-Charry Giovanni , Gómez-Mendoza Juan-Bernardo
Published: May 2022

Although many friction models have been developed by various researchers over the years, a general model does not exist yet. In this article, we present the work that was developed to identify and propose an empirical model that represents the behavior of friction in a cable-driven transmission. We designed and built a test bench that allowed us to study the incidence of friction as evidenced by the type of cable thread, the dimensions of the pulley and the capstan and the application of an external load for pulley-cable type transmissions in a variety of configurations. The results obtained show a marked influence of the external load on friction behavior. Based on these results, we propose a friction model that involves the external load and the transmission ratio. During the validation of the model, it was found that the proposed model reproduced the friction behavior better than the LuGre model, obtaining errors up to 44% lower, mainly for high loads and low speeds.
A Global Path Planning Method for Unmanned Ground Vehicles in Off-Road Environments Based on Mobility Prediction
Authors: Hua Chen , Niu Runxin , Yu Biao , Zheng Xiaokun , Bai Rengui , Zhang Song
Published: May 2022

In a complex off-road environment, due to the low bearing capacity of the soil and the uneven features of the terrain, generating a safe and effective global route for unmanned ground vehicles (UGVs) is critical for the success of their motion and mission. Most traditional global path planning methods simply take the shortest path length as the optimization objective, which makes it difficult to plan a feasible and safe route in complex off-road environments. To address this problem, this research proposes a global path planning method, which considers the influence of terrain factors and soil mechanics on UGV mobility. First, we established a high-resolution 3D terrain model with remote sensing elevation terrain data, land use and soil type distribution data, based on a geostatistical method. Second, we analyzed the vehicle mobility by the terramechanical method (i.e., vehicle cone index and Bakker’s theory), and then calculated the mobility cost based on a fuzzy inference method. Finally, based on the calculated mobility cost, the probabilistic roadmap method was used to establish the connected matrix and the multi-dimensional traffic cost evaluation matrix among the sampling nodes, and then an improved A* algorithm was proposed to generate the global route.
Comprehensive and Simplified Fault Diagnosis for Three-Phase Induction Motor Using Parity Equation Approach in Stator Current Reference Frame
Authors: Rodriguez-Blanco Marco Antonio , Golikov Victor , Vazquez-Avila Jose Luis , Samovarov Oleg , Sanchez-Lara Rafael , Osorio-Sánchez René , Pérez-Ramírez Agustín
Published: May 2022

In this paper, a complementary and simplified scheme to diagnose electrical faults in a three-phase induction motor using the parity equations approach during steady state operation bases on the stator current reference frame is presented. The proposed scheme allows us to identify the motor phase affected due to faults related to the stator side, such as current sensors, voltage sensors, and resistance. The results obtained in this work complement a detection system that uses the DQ model of the three-phase induction motor and parity equations focused on the synchronous reference frame, which can detect stator-side faults but cannot locate the affected phase. In addition, considering practical and operational aspects, the residual detection set obtained is simplified to three simple algebraic equations that are easy to implement. The simulation results using the PSIM simulation software and the experimental test allow us to validate the proposed scheme.
Shear Failure Mechanism and Numerical Simulation Analysis of Rock-like Materials with an Embedded Flaw
Authors: Teng Mingyang , Li Jiashen , Lian Shuailong , Bi Jing , Wang Chaolin
Published: May 2022

In this study, the failure characteristics of self-made rock with internal flaws under shear were studied and a numerical simulation analysis was carried out. Firstly, based on basic physical and mechanical tests, the shear strength characteristics of rocks with built-in 3D defects were summarized. PFC3D simulation software was used to model the samples with flaws, and the microscopic parameters were calibrated according to the test results. From the simulation results, it was found that the generation mode of microcracks from the flaw tip was different. The microcracks of forward shear and reverse shear were mainly generated from the horizontal direction, while the microcracks of lateral shear gradually increased from the upper and lower ends of the flaw in the opposite direction. When the peak shear strength was reached, the total number of cracks was the largest in lateral shear and the smallest in forward shear. When studying the particle velocity vector field, it was found that when reaching the peak shear strength, the particles on both sides of the prefabricated flaw appeared to be in vortex motion. When α = 45° and σn = 2 MPa, the failure mode of forward shear and lateral shear was shear-tensile-shear (S-T-S), and that of reverse shear and the intact specimen was shear-shear-shear (S-S-S). The lateral shear tensile effect was the most obvious and was mainly concentrated in the middle part of the sample.
Thesis Review: The Manifestation of Race in Everyday Communication Interactions in New Zealand
Authors: Henson Donna
Published: May 2022

In the second of the Theses Review series Dr Donna Henson reviews the work of Elizabeth Revell. ‘This thesis presents an interesting and thoughtful autoethnographic inquiry into the manifestation of race in everyday communication interactions in New Zealand. An uncommon choice of both topic and method in the local communication disciplinary context, Revell presents a partial collaborative autoethnographic approach to the study that entails the reflexive analysis of qualitative data drawn from solicited participant diaries, semi-structured interviews and focus groups.
Thesis Review: Gender, Migration and Communication Networks
Authors: Ayallo Irene
Published: May 2022

In this thesis, reviewed by Irene Ayallo, ‘the author investigates the role of communication networks in the pre-and post-migration process of Latin American women resettled in New Zealand. This well-researched and skillfully written thesis begin from the premise that while the process of migration and resettlement is complex and challenging for all migrants, it is more demanding for women.
Thesis review: The storytellers: Identity narratives by New Zealand African youth – participatory visual methodological approach to situating identity, migration and representation by Makanaka Tuwe
Authors: Norris Adele
Published: May 2022

This fascinating and original work explores the experiences of third-culture children of African descent in New Zealand. The term ‘third-culture kid’ refers to an individual who grows up in a culture different from the culture of their parents. Experiences of youth of African descent is under-researched in New Zealand. The central research focus explores racialised emotions internalised by African youth that are largely attributed to a lack of positive media representation of African and/or black youth, coupled with daily experiences of micro-aggressions and structural racism. In this respect, the case-study analysis is reflective of careful, methodological and deliberative analysis, which offers powerful insights into the grass-roots strategies employed by African youth to resist negative stereotypes that problematise and marginalise them politically and economically.
Exploring the role of Buddhist monks’ and nuns’ engagement in community development as catalysts for social change and sustainable development in Lao People’s Democratic Republic: A case study of the Buddhism for Development Project at Ban Bungsanthueng, Nongbok District, Khammouane Province, by Toung Eh Synuanchanh
Authors: Seneviratne Kalinga
Published: May 2022

The topic of this research report is an important one in the context of Asia’s rapid economic development in recent years, and the need to rethink development policy and especially methodologies of development communications, so the mistakes of the past will not be replicated. Thus, the study is an important initiative at this period of time. The research takes as a case study the Buddhism for Development Project (BDP) implemented at Ban Bungsanthueng village in the Khammouane Province by its Buddhist Volunteer Spirit for Community network (BVSC network). The fieldwork took place at the BDP’s training centre in Vientiane and the Buddhist initiatives at Ban Bungsanthueng. The research demonstrates how the BDP and its network apply participatory approaches through interpersonal communication, such as sermon delivery, Dhamma (Buddhist teachings) talk, and daily interaction with villagers and project members.