bluuebunny/crossref_metadata_2025 huggingface dataset:
Prenatal management of fetal intrapericardial teratoma: a systematic review
Authors: Nassr Ahmed A. , Shazly Sherif A. , Morris Shaine A. , Ayres Nancy , Espinoza Jimmy , Erfani Hadi , Olutoye Olutoyin A. , Sexson Sara K. , Olutoye Oluyinka O. , Fraser Charles D. , Belfort Michael A. , Shamshirsaz Alireza A.
Published: July 2017

Objectives

The purpose of this systematic review is to provide a comprehensive overview on the clinical course, perinatal outcome, and effectiveness of prenatal management options for pericardial teratoma.

Methods

A comprehensive search including Ovid MEDLINE, Ovid EMBASE, and Scopus was conducted from inception to September 2016. All studies that reported the prenatal course of pericardial teratoma in singleton or twin gestations were considered eligible. Standardized forms were used for data abstraction by two independent reviewers.

Results

Out of 217 screened abstracts, 59 studies reporting 67 fetuses with pericardial teratoma were included. Twenty‐three singleton fetuses and 3 fetuses in twin gestations underwent prenatal treatment, and 20 (76.9%) of them were hydropic at the time of intervention. Of those, 15/20 (75%) had a favorable outcome. In the non‐intervention group (n = 41), 26 (63.4%) developed hydrops, and out of those, 8 (30.8%) had a favorable outcome.

Conclusion

Prenatal fluid drainage and other prenatal techniques have been utilized in the treatment of intrapericardial teratoma. While most fetuses tolerated pericardiocentesis, the neonatal benefit of this procedure is still uncertain, and outcomes of other interventions had variable success. Prenatal intervention for pericardial teratoma may be an option in specialized units but, given the maternal and fetal risks, needs careful consideration. © 2017 John Wiley & Sons, Ltd.
Aortic isthmus shunt dynamics in normal and complicated monochorionic pregnancies
Authors: Seravalli Viola , Block‐Abraham Dana , McShane Cyrethia , Millard Sarah , Baschat Ahmet , Miller Jena
Published: July 2017

Objective

To study fetal aortic isthmus (AoI) shunt dynamics in monochorionic (MC) twins.

Methods

Normal and complicated MC pregnancies were prospectively enrolled. The relationship of isthmus flow index (IFI) with Doppler parameters of umbilical artery (UA), descending aorta, middle cerebral artery, and ductus venosus and with left and right ventricular cardiac output and stroke volume was studied.

Results

The IFI was obtained in 180 examinations from 48 pregnancies (24 twin–twin transfusion syndrome, TTTS; 4 selective intrauterine growth restriction, sIUGR; 12 TTTS + sIUGR; and 8 uncomplicated). Median gestational age was 20.9 weeks. AoI diastolic flow was reversed in three cases. UA pulsatility index (PI) and ductus venosus‐PI z‐scores were negatively correlated with the IFI (rs −0.40 and −0.26, respectively, p < 0.001). Regression analysis identified only UA‐PI as a determinant of the IFI (p < 0.001). The IFI was significantly correlated with left ventricular cardiac output and stroke volume. It did not differ between TTTS donors and recipients. sIUGR fetuses had significantly lower IFI compared with normal‐grown counterparts (p < 0.001).

Conclusion

In MC gestations, AoI shunting is predominantly determined by placental flow resistance, while cerebral impedance and volume status have no impact. In MC twins, the relationship between AoI flow and outcome deserves further study in the setting of sIUGR. © 2017 John Wiley & Sons, Ltd.
MCDA swing weighting and discrete choice experiments for elicitation of patient benefit‐risk preferences: a critical assessment
Authors: Tervonen Tommi , Gelhorn Heather , Sri Bhashyam Sumitra , Poon Jiat‐Ling , Gries Katharine S. , Rentz Anne , Marsh Kevin
Published: July 2017

Purpose

Multiple criteria decision analysis swing weighting (SW) and discrete choice experiments (DCE) are appropriate methods for capturing patient preferences on treatment benefit‐risk trade‐offs. This paper presents a qualitative comparison of the 2 methods.

Methods

We review and critically assess similarities and differences of SW and DCE based on 6 aspects: comprehension by study participants, cognitive biases, sample representativeness, ability to capture heterogeneity in preferences, reliability and validity, and robustness of the results.

Results

The SW choice task can be more difficult, but the workshop context in which SW is conducted may provide more support to patients who are unfamiliar with the end points being evaluated or who have cognitive impairments. Both methods are similarly prone to a number of biases associated with preference elicitation, and DCE is prone to simplifying heuristics, which limits its application with large number of attributes. The low cost per patient of the DCE means that it can be better at achieving a representative sample, though SW does not require such large sample sizes due to exact nature of the collected preference data. This also means that internal validity is automatically enforced with SW, while the internal validity of DCE results needs to be assessed manually.

Conclusions

Choice between the 2 methods depends on characteristics of the benefit‐risk assessment, especially on how difficult the trade‐offs are for the patients to make and how many patients are available. Although there exist some empirical studies on many of the evaluation aspects, critical evidence gaps remain.
Polymers as drugs—Advances in therapeutic applications of polymer binding agents
Authors: Connor Eric F. , Lees Inez , Maclean Derek
Published: July 2017

Polymer drugs are those in which a polymer agent has a direct therapeutic effect on the body. A major investigated area of polymer drugs is their use as binding agents, or sequestrants, which can neutralize or remove undesired components from the body. By taking advantage of the unique properties of crosslinked, insoluble polymers, new polymer drugs continue to be developed for oral use, acting in the gastrointestinal tract and excreted in the feces. Soluble polymer binders may be administered by a variety of routes and act at diverse sites of therapeutic action. This article reviews the properties of polymer drugs, particularly sequestrants, with an emphasis on recent advances in polymer properties which may enhance the utility of this class of drugs. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 3146–3157
Managing fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), with Bt maize and insecticides in southern Brazil
Authors: Burtet Leonardo M , Bernardi Oderlei , Melo Adriano A , Pes Maiquel P , Strahl Thiago T , Guedes Jerson VC
Published: July 2017

BACKGROUND

Maize plants expressing insecticidal proteins of Bacillus thuringiensis are valuable options for managing fall armyworm (FAW), Spodoptera frugiperda, in Brazil. However, control failures were reported, and therefore insecticides have been used to control this species. Based on these, we evaluated the use of Bt maize and its integration with insecticides against FAW in southern Brazil.

RESULTS

Early‐planted Agrisure TL, Herculex, Optimum Intrasect and non‐Bt maize plants were severely damaged by FAW and required up to three insecticidal sprays. In contrast, YieldGard VT Pro, YieldGard VT Pro 3, PowerCore, Agrisure Viptera and Agrisure Viptera 3 showed little damage and did not require insecticides. Late‐planted Bt maize plants showed significant damage by FAW and required up to four sprays, with the exceptions of Agrisure Viptera and Agrisure Viptera 3. Exalt (first and second sprays); Lannate + Premio (first spray) and Avatar (second spray); and Karate + Match (first spray) and Ampligo (second spray) were the most effective insecticides against FAW larvae in Bt and non‐Bt maize.

CONCLUSION

Maize plants expressing Cry proteins exhibited FAW control failures in southern Brazil, necessitating insecticidal sprays. In contrast, Bt maize containing the Vip3Aa20 protein remained effective against FAW. However, regardless of the insecticide used against FAW surviving on Bt maize, grain yields were similar. © 2017 Society of Chemical Industry
High‐resolution mass spectrometric analysis of myo‐inositol hexakisphosphate using electrospray ionisation Orbitrap
Authors: McIntyre Catherine A. , Arthur Christopher J. , Evershed Richard P.
Published: July 2017

Rationale

The phosphorus storage compound in grains, phytic acid, or myo‐inositol hexakisphosphate (IP6), is important for nutrition and human health, and is reportedly the most abundant organic phosphorus compound in soils. Methods for its determination have traditionally relied on complexation with iron and precipitation, acid digestion and measurement of phosphate concentration, or 31P NMR spectroscopy. Direct determination of phytic acid (and its homologues) using mass spectrometry has, as yet, found limited application to environmental or other complex matrices. The behaviour of phytic acid in electrospray ionisation high‐resolution mass spectrometry (ESI‐HRMS) and its fragmentation, both in‐source and via collision‐induced dissociation, have not been studied so far.

Methods

The negative ion mass spectrometry and tandem mass spectrometry (MS/MS) of IP6, and the lower inositol pentakisphosphate (IP5), using an ESI‐Orbitrap mass spectrometer is described. The purity of the compounds was investigated using anion‐exchange chromatography.

Results

IP6 is highly anionic, forming multiply charged ions and sodium adduct ions, which readily undergo dissociation in the ESI source. MS/MS analysis of the phytic acid [M−2H]2− ion and fragment ions and comparison with the full MS of the IP5 reference standard, and the MS/MS spectrum of the pentakisphosphate [M−2H]2− ion, confirm the fragmentation pattern of inositol phosphates in ESI. Further evidence for dissociation in the ion source is shown by the effect of increasing the source voltage on the mass spectrum of phytic acid.

Conclusions

The ESI‐HRMS of inositol phosphates is unusual and highly characteristic. The study of the full mass spectrum of IP6 in ESI‐HRMS mode indicates the detection of the compound in environmental matrices using this technique is preferable to the use of multiple reaction monitoring (MRM).
Determination of uranium isotope ratios using a liquid sampling atmospheric pressure glow discharge/Orbitrap mass spectrometer system
Authors: Hoegg Edward D. , Marcus R. Kenneth , Koppenaal David W. , Irvahn Jan , Hager George J. , Hart Garret L.
Published: July 2017

Rationale

The field of highly accurate and precise isotope ratio analysis, for use in nonproliferation, has been dominated by thermal ionization and inductively coupled plasma mass spectrometry. While these techniques are considered the gold standard for isotope ratio analysis, a downsized instrument capable of accurately and precisely measuring uranium (U) isotope ratios is desirable for field studies or in laboratories with limited infrastructure.

Methods

The developed system interfaces the liquid sampling, an atmospheric pressure glow discharge (LS‐APGD) ion source, with a high‐resolution Exactive Orbitrap mass spectrometer. With this experimental setup certified U isotope standards and unknown samples were analyzed. The accuracy and precision of the system were then determined.

Results

The LS‐APGD/Exactive instrument measured a certified reference material of natural U (235U/238U = 0.007261) with a 235U/238U ratio of 0.007065 and a % relative standard uncertainty of 0.082, meeting the International Target Values for the destructive analysis of U. In addition, when three unknowns were measured and these measurements were compared with the results from an ICP multi‐collector instrument, there were no statistical differences between the two instruments.

Conclusions

The LS‐APGD/Orbitrap system, while still in the preliminary stages of development, offers highly accurate and precise isotope ratio results that suggest a potential paradigm shift in the world of isotope ratio analysis. Furthermore, the portability of the LS‐APGD as an elemental ion source, combined with the small size and smaller operating demands of the Orbitrap, suggests that the instrumentation is capable of being field‐deployable.
Classroom Talk as Writing Instruction for Learning to Make Writing Moves in Literary Arguments
Authors: VanDerHeide Jennifer
Published: July 2017

Although teaching argumentative writing in schools is often about teaching argumentative forms, this instructional approach limits students’ flexibility and choice as writers, readers, and meaning makers. An alternative method, rooted in tenets of genre theory, offers a different approach. Rather than treating argument as a static form, genre theory assumes that genres (including argumentative genres) are situated, typified ways that people make moves through writing to accomplish goals through language. Taking a genre theory and sociocultural, discourse lens, this article explores how a teacher in an Advanced Placement Literature course approached the teaching of argumentative writing. Through a moves analysis of focal student essays and a discourse analysis of classroom talk, the study asks two questions: (1) How does the teacher, through classroom talk, support students in making moves of literary argument? (2) How do students make these moves in speaking and writing? Findings identify argumentative moves and submoves that students made in their argumentative essays. The teacher supported students’ learning by explicitly pointing to these moves in model essays, posing questions that prompted students to make the same moves in classroom talk, and revoicing student responses in more disciplinary ways. Through small‐ and large‐group discussions, students learned to make moves that they later made in essays. In identifying classroom talk moves that serve argumentative writing instruction in literary studies, these findings have implications for classroom‐based argument writing instruction, for literacy teacher preparation and professional development, and for future research on approaches to teaching literature and writing.
Nematic DNA Thermotropic Liquid Crystals with Photoresponsive Mechanical Properties
Authors: Zhang Lei , Maity Sourav , Liu Kai , Liu Qing , Göstl Robert , Portale Giuseppe , Roos Wouter H. , Herrmann Andreas
Published: July 2017

Over the last decades, water‐based lyotropic liquid crystals of nucleic acids have been extensively investigated because of their important role in biology. Alongside, solvent‐free thermotropic liquid crystals (TLCs) from DNA are gaining great interest, owing to their relevance to DNA‐inspired optoelectronic applications. Up to now, however, only the smectic phase of DNA TLCs has been reported. The development of new mesophases including nematic, hexagonal, and cubic structures for DNA TLCs remains a significant challenge, which thus limits their technological applications considerably. In this work, a new type of DNA TLC that is formed by electrostatic complexation of anionic oligonucleotides and cationic surfactants containing an azobenzene (AZO) moiety is demonstrated. DNA–AZO complexes form a stable nematic mesophase over a temperature range from −7 to 110 °C and retain double‐stranded DNA structure at ambient temperature. Photoisomerization of the AZO moieties from the E‐ to the Z‐form alters the stiffness of the DNA–AZO hybrid materials opening a pathway toward the development of DNA TLCs as stimuli‐responsive biomaterials.
A Solar‐Blind UV Detector Based on Graphene‐Microcrystalline Diamond Heterojunctions
Authors: Wei Minsong , Yao Kaiyuan , Liu Yumeng , Yang Chen , Zang Xining , Lin Liwei
Published: July 2017

An ultraviolet detector is demonstrated through a whole‐wafer, thin diamond film transfer process to realize the heterojunction between graphene and microcrystalline diamond (MCD). Conventional direct transfer processes fail to deposit graphene onto the top surface of the MCD film. However, it is found that the 2 µm thick MCD diamond film can be easily peeled off from the growth silicon substrate to expose its smooth backside for the graphene transfer process for high‐quality graphene/MCD heterojunctions. A vertical graphene/MCD/metal structure is constructed as the photodiode device using graphene as the transparent top electrode for solar‐blind ultraviolet sensing with high responsivity and gain factor. As such, this material system and device architecture could serve as the platform for next‐generation optoelectronic systems.
Brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT3) levels in post-mortem brain tissue from patients with depression compared to healthy individuals – a proof of concept study
Authors: Sheldrick A. , Camara S. , Ilieva M. , Riederer P. , Michel T.M.
Published: July 2017

The neurotrophic factors (NTF) hypothesis of depression was postulated nearly a decade ago and is nowadays widely acknowledged. Previous reports suggest that cerebral concentrations of NTF may be reduced in suicide victims who received minimal or no antidepressant pharmacotherapy. Recent evidence suggests that antidepressant treatment may improve or normalise cerebral concentrations of neurotrophic factors. Therefore, we examined the concentration of brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT3) in different brain regions (cortex, cingulate gyrus, thalamus, hippocampus, putamen and nucleus caudatus) of 21 individuals – 7 patients of which 4 patients with major depressive disorder (MDD) and overall age 86.8 ± 5 years who received antidepressant pharmacotherapy (selective serotonin re-uptake inhibitors [SSRI]; tricyclic antidepressants [TCA]), 3 patients with MDD without antidepressant treatment and overall age 84.3 ± 5 years versus 14 unaffected subjects at age 70.3 ± 13.8. We detected significant elevation of BDNF (parietal cortex) and NT3 (parietal, temporal and occipital cortex, cingulate gyrus, thalamus, putamen and nucleus caudatus regions) in MDD patients who received antidepressant medication compared to MDD untreated patients and controls. Moreover, we detected a significant decrease of NT3 levels in the parietal cortex of patients suffering from MDD non-treated patients without treatment compared to healthy individuals. Although the limited statistical power due to the small sample size in this proof of concept study corroborates data from previous studies, which show that treatment with antidepressants mediates alterations in neuroplasticity via the action of NTF. However, more research using post-mortem brain tissue with larger samples needs to be carried out as well as longitudinal studies to further verify these results.
‘The situation over there really bothers me’: Ronald Reagan and the Northern Ireland conflict
Authors: Cooper James
Published: July 2017

The relationship between the Reagan administration and the Northern Ireland conflict is a neglected area of transatlantic history. This article addresses the extent of Ronald Reagan’s interest in the Northern Irish conflict and the manner in which other protagonists sought to secure or prevent his involvement. It will examine the president’s approach in the context of different views within his administration, the State Department’s wish to maintain American neutrality on the issue of Northern Ireland, and the desire of leading Irish-American politicians for the American government to be much more interventionist. These debates coincided with significant developments in Northern Ireland. Therefore, Reagan’s contribution to the Anglo–Irish process encapsulates a variety of issues: the Troubles in Northern Ireland during the 1980s, the 1985 Anglo–Irish Agreement and the internationalisation of the conflict before the election of President Bill Clinton in 1993.
Passive scalar mixing and decay at finite correlation times in the Batchelor regime
Authors: Aiyer Aditya K. , Subramanian Kandaswamy , Bhat Pallavi
Published: July 2017

An elegant model for passive scalar mixing and decay was given by Kraichnan (Phys. Fluids, vol. 11, 1968, pp. 945–953) assuming the velocity to be delta correlated in time. For realistic random flows this assumption becomes invalid. We generalize the Kraichnan model to include the effects of a finite correlation time, $\unicode[STIX]{x1D70F}$, using renewing flows. The generalized evolution equation for the three-dimensional (3-D) passive scalar spectrum $\hat{M}(k,t)$ or its correlation function $M(r,t)$, gives the Kraichnan equation when $\unicode[STIX]{x1D70F}\rightarrow 0$, and extends it to the next order in $\unicode[STIX]{x1D70F}$. It involves third- and fourth-order derivatives of $M$ or $\hat{M}$ (in the high $k$ limit). For small-$\unicode[STIX]{x1D70F}$ (or small Kubo number), it can be recast using the Landau–Lifshitz approach to one with at most second derivatives of $\hat{M}$. We present both a scaling solution to this equation neglecting diffusion and a more exact solution including diffusive effects. To leading order in $\unicode[STIX]{x1D70F}$, we first show that the steady state 1-D passive scalar spectrum, preserves the Batchelor (J. Fluid Mech., vol. 5, 1959, pp. 113–133) form, $E_{\unicode[STIX]{x1D703}}(k)\propto k^{-1}$, in the viscous–convective limit, independent of $\unicode[STIX]{x1D70F}$. This result can also be obtained in a general manner using Lagrangian methods. Interestingly, in the absence of sources, when passive scalar fluctuations decay, we show that the spectrum in the Batchelor regime at late times is of the form $E_{\unicode[STIX]{x1D703}}(k)\propto k^{1/2}$ and also independent of $\unicode[STIX]{x1D70F}$. More generally, finite $\unicode[STIX]{x1D70F}$ does not qualitatively change the shape of the spectrum during decay. The decay rate is however reduced for finite $\unicode[STIX]{x1D70F}$. We also present results from high resolution ($1024^{3}$) direct numerical simulations of passive scalar mixing and decay. We find reasonable agreement with predictions of the Batchelor spectrum during steady state. The scalar spectrum during decay is however dependent on initial conditions. It agrees qualitatively with analytic predictions when power is dominantly in wavenumbers corresponding to the Batchelor regime, but is shallower when box-scale fluctuations dominate during decay.
Modeling adult emergence and fecundity of factitious hosts under different food sources supports massive egg production management
Authors: Tavares J. , Silva L. , Oliveira L.
Published: July 2017

Ephestia kuehniella(Lepidoptera, Pyralidae) andSitotroga cerealella(Lepidoptera, Gelechiidae) are important factitious hosts used for production of biological control agents. Their differences in terms of biology and behavior require adjustments in their mass production, particularly when using corn or barley as food in grain or in bran. We modeled adult emergence, oviposition period and egg production along time after emergence, as a function of the food source. Significant differences between hosts or food type were found for these variables and for adult weight but not for sex ratio. Our results confirm the possibility of mass production of these hosts using corn or barley as food source. Integrating adult emergence patterns and age specific fecundity patterns into a single model, it is clear that rearingE. kuehniellaon barley would result in the highest egg output in much shorter time thanE. kuehniellaon corn orS. cerealellaon barley.
Angiostrongylus mackerrasae and A. cantonensis (Nematoda: Metastrongyloidea) belong to same genetic lineage: evidence from mitochondrial protein-coding genes
Authors: Song S.-L. , Yong H.-S. , Eamsobhana P.
Published: July 2017

Angiostrongylus mackerrasae is a parasitic nematode of rats found in Australia. When first reported, it was referred to as A. cantonensis. Recent molecular studies, including the mitochondrial genome, indicate that it is highly similar to A. cantonensis. These studies did not include A. malaysiensis, another member of the A. cantonensis species complex, for comparison. The present study examined the genetic distance and phylogenetic relationship between the component taxa (A. cantonensis, A. mackerrasae and A. malaysiensis) of the A. cantonensis species complex, based on the 12 protein-coding genes (PCGs) of their mitochondrial genome. Both the nucleotide and amino acid sequences were analysed. Angiostrongylus mackerrasae and A. cantonensis are members of the same genetic lineage and both are genetically distinct from A. malaysiensis. The genetic distance based on concatenated nucleotide sequences of 12 mt-PCGs between A. mackerrasae and A. cantonensis from Thailand is p = 1.73%, while that between the Thai and Chinese taxa of A. cantonensis is p = 3.52%; the genetic distance between A. mackerrasae and A. cantonensis from China is p = 3.70%. The results indicate that A. mackerrasae and A. cantonensis belong to the same genetic lineage, and that A. mackerrasae may be conspecific with A. cantonensis. It remains to be resolved whether A. mackerrasae is conspecific with A. cantonensis or undergoing incipient speciation.
Optimising the use of otowicks in otitis externa
Authors: Bola S , Rashid M , Hickey S
Published: July 2017

Objective:

Otowicks are used to treat otitis externa with significant ear canal oedema. This study investigates how well drops penetrate through to reach the deep canal and whether it is safe to leave otowicks in the canal for more than 2 days.

Methods:

Sterile otowicks were inserted into mock ear canals and vertically over pseudomonas-seeded agar plates whilst gentamicin or ciprofloxacin drops were administered. The time taken for drops to penetrate through the otowick was recorded. Separately, pseudomonas-seeded otowicks were treated with saline or antibacterial drops. The penetrating drops were observed for bacterial growth on sterile agar.

Results:

It took six drops before penetration occurred for both antibiotics. When sterile saline drops were applied to bacterially contaminated otowicks, the penetrating drops displayed bacterial growth on agar, indicating that pseudomonas penetrated through the otowick. However, when antibiotic drops were applied, penetrating drops showed no bacterial growth on the corresponding agar plate.

Conclusion:

Bacteria can penetrate otowicks but this is prevented by continuous application of antibacterial ear drops. Ear wicks need priming with six drops before starting a regimen, so that the initial dose is fully absorbed.
Constituting Animacy and Community in a Terminal Formative Bundled Offering from the Coast of Oaxaca, Mexico
Authors: Brzezinski Jeffrey S. , Joyce Arthur A. , Barber Sarah B.
Published: July 2017

In this paper, we examine a Terminal Formative-period (150 bc–ad 250) bundled offering from the site of Cerro de la Virgen, located on the Pacific coast of Oaxaca, Mexico. The offering was emplaced below a prominent public building in the site's ceremonial centre and contained five stone objects, including a rain deity mask, a fragment of a second mask, a figurine of a deceased ancestor and two miniature table altars, as well as nine small ceramic vessels. Considered together as a ‘sacred bundle’, the stone objects collectively reference agricultural fertility, rulership and ancestor veneration, which we interpret to be a metaphorical invocation of a fundamental tenet of prehispanic Mesoamerican religious belief—the sacred covenant. The offering also played an active part in founding the community of Cerro de la Virgen, connecting its residents with the divine, the ancestors and the outside world and constituting differences in status among its members.
Disentangling environmental and spatial effects on phylogenetic structure of angiosperm tree communities in China
Authors: Qian Hong , Chen Shengbin , Zhang Jin-Long
Published: July 2017

Niche-based and neutrality-based theories are two major classes of theories explaining the assembly mechanisms of local communities. Both theories have been frequently used to explain species diversity and composition in local communities but their relative importance remains unclear. Here, we analyzed 57 assemblages of angiosperm trees in 0.1-ha forest plots across China to examine the effects of environmental heterogeneity (relevant to niche-based processes) and spatial contingency (relevant to neutrality-based processes) on phylogenetic structure of angiosperm tree assemblages distributed across a wide range of environment and space. Phylogenetic structure was quantified with six phylogenetic metrics (i.e., phylogenetic diversity, mean pairwise distance, mean nearest taxon distance, and the standardized effect sizes of these three metrics), which emphasize on different depths of evolutionary histories and account for different degrees of species richness effects. Our results showed that the variation in phylogenetic metrics explained independently by environmental variables was on average much greater than that explained independently by spatial structure, and the vast majority of the variation in phylogenetic metrics was explained by spatially structured environmental variables. We conclude that niche-based processes have played a more important role than neutrality-based processes in driving phylogenetic structure of angiosperm tree species in forest communities in China.
Spectral characteristics of cotton seeds treated by a dielectric barrier discharge plasma
Authors: Wang Xing-Quan , Zhou Ren-Wu , Groot Gerard de , Bazaka Kateryna , Murphy Anthony B. , Ostrikov Kostya
Published: July 2017

Cold atmospheric plasma has recently emerged as a simple, low-cost and efficient physical method for inducing significant biological responses in seeds and plants without the use of traditional, potentially environmentally-hazardous chemicals, fungicides or hormones. While the beneficial effects of plasma treatment on seed germination, disease resistance and agricultural output have been reported, the mechanisms that underpin the observed biological responses are yet to be fully described. This study employs Fourier Transform Infrared (FTIR) spectroscopy and emission spectroscopy to capture chemical interactions between plasmas and seed surfaces with the aim to provide a more comprehensive account of plasma−seed interactions. FTIR spectroscopy of the seed surface confirms plasma-induced chemical etching of the surface. The etching facilitates permeation of water into the seed, which is confirmed by water uptake measurements. FTIR of exhaust and emission spectra of discharges show oxygen-containing species known for their ability to stimulate biochemical processes and deactivate pathogenic microorganisms. In addition, water gas, CO2, CO and molecules containing −C(CH3)3− moieties observed in FTIR spectra of the exhaust gas during plasma treatment may be partly responsible for the plasma chemical etching of seed surface through oxidizing the organic components of the seed coat.
Genome reconstruction in Cynara cardunculus taxa gains access to chromosome-scale DNA variation
Authors: Acquadro Alberto , Barchi Lorenzo , Portis Ezio , Mangino Giulio , Valentino Danila , Mauromicale Giovanni , Lanteri Sergio
Published: July 2017

The genome sequence of globe artichoke (Cynara cardunculus L. var. scolymus, 2n = 2x = 34) is now available for use. A survey of C. cardunculus genetic resources is essential for understanding the evolution of the species, carrying out genetic studies and for application of breeding strategies. We report on the resequencing analyses (~35×) of four globe artichoke genotypes, representative of the core varietal types, as well as a genotype of the related taxa cultivated cardoon. The genomes were reconstructed at a chromosomal scale and structurally/functionally annotated. Gene prediction indicated a similar number of genes, while distinctive variations in miRNAs and resistance gene analogues (RGAs) were detected. Overall, 23,5 M SNP/indel were discovered (range 6,34 M –14,50 M). The impact of some missense SNPs on the biological functions of genes involved in the biosynthesis of phenylpropanoid and sesquiterpene lactone secondary metabolites was predicted. The identified variants contribute to infer on globe artichoke domestication of the different varietal types, and represent key tools for dissecting the path from sequence variation to phenotype. The new genomic sequences are fully searchable through independent Jbrowse interfaces (www.artichokegenome.unito.it), which allow the analysis of collinearity and the discovery of genomic variants, thus representing a one-stop resource for C. cardunculus genomics.
Glucagon-like peptide-1 reduces pancreatic β-cell mass through hypothalamic neural pathways in high-fat diet-induced obese rats
Authors: Ando Hisae , Gotoh Koro , Fujiwara Kansuke , Anai Manabu , Chiba Seiichi , Masaki Takayuki , Kakuma Tetsuya , Shibata Hirotaka
Published: July 2017

We examined whether glucagon-like peptide-1 (GLP-1) affects β-cell mass and proliferation through neural pathways, from hepatic afferent nerves to pancreatic efferent nerves via the central nervous system, in high-fat diet (HFD)-induced obese rats. The effects of chronic administration of GLP-1 (7–36) and liraglutide, a GLP-1 receptor agonist, on pancreatic morphological alterations, c-fos expression and brain-derived neurotrophic factor (BDNF) content in the hypothalamus, and glucose metabolism were investigated in HFD-induced obese rats that underwent hepatic afferent vagotomy (VgX) and/or pancreatic efferent sympathectomy (SpX). Chronic GLP-1 (7–36) administration to HFD-induced obese rats elevated c-fos expression and BDNF content in the hypothalamus, followed by a reduction in pancreatic β-cell hyperplasia and insulin content, thus resulting in improved glucose tolerance. These responses were abolished by VgX and SpX. Moreover, administration of liraglutide similarly activated the hypothalamic neural pathways, thus resulting in a more profound amelioration of glucose tolerance than native GLP-1 (7–36). These data suggest that GLP-1 normalizes the obesity-induced compensatory increase in β-cell mass and glucose intolerance through a neuronal relay system consisting of hepatic afferent nerves, the hypothalamus, and pancreatic efferent nerves.
Imaging mass spectrometry and MS/MS molecular networking reveals chemical interactions among cuticular bacteria and pathogenic fungi associated with fungus-growing ants
Authors: Boya P. Cristopher A. , Fernández-Marín Hermógenes , Mejía Luis C. , Spadafora Carmenza , Dorrestein Pieter C. , Gutiérrez Marcelino
Published: July 2017

The fungus-growing ant-microbe symbiosis is an ideal system to study chemistry-based microbial interactions due to the wealth of microbial interactions described, and the lack of information on the molecules involved therein. In this study, we employed a combination of MALDI imaging mass spectrometry (MALDI-IMS) and MS/MS molecular networking to study chemistry-based microbial interactions in this system. MALDI IMS was used to visualize the distribution of antimicrobials at the inhibition zone between bacteria associated to the ant Acromyrmex echinatior and the fungal pathogen Escovopsis sp. MS/MS molecular networking was used for the dereplication of compounds found at the inhibition zones. We identified the antibiotics actinomycins D, X2 and X0β, produced by the bacterium Streptomyces CBR38; and the macrolides elaiophylin, efomycin A and efomycin G, produced by the bacterium Streptomyces CBR53.These metabolites were found at the inhibition zones using MALDI IMS and were identified using MS/MS molecular networking. Additionally, three shearinines D, F, and J produced by the fungal pathogen Escovopsis TZ49 were detected. This is the first report of elaiophylins, actinomycin X0β and shearinines in the fungus-growing ant symbiotic system. These results suggest a secondary prophylactic use of these antibiotics by A. echinatior because of their permanent production by the bacteria.
Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration
Authors: Hong Xuechong , Margariti Andriana , Le Bras Alexandra , Jacquet Laureen , Kong Wei , Hu Yanhua , Xu Qingbo
Published: July 2017

Endothelial dysfunction is widely implicated in cardiovascular pathological changes and development of vascular disease. In view of the fact that the spontaneous endothelial cell (EC) regeneration is a slow and insufficient process, it is of great interest to explore alternative cell sources capable of generating functional ECs. Vascular smooth muscle cell (SMC) composes the majority of the vascular wall and retains phenotypic plasticity in response to various stimuli. The aim of this study is to test the feasibility of the conversion of SMC into functional EC through the use of reprogramming factors. Human SMCs are first dedifferentiated for 4 days to achieve a vascular progenitor state expressing CD34, by introducing transcription factors OCT4, SOX2, KLF4 and c-MYC. These SMC-derived progenitors are then differentiated along the endothelial lineage. The SMC-converted ECs exhibit typical endothelial markers expression and endothelial functions in vitro, in vivo and in disease model. Further comprehensive analysis indicates that mesenchymal-to-epithelial transition is requisite to initiate SMCs reprogramming into vascular progenitors and that members of the Notch signalling pathway regulate further differentiation of the progenitors into endothelial lineage. Together, we provide the first evidence of the feasibility of the conversion of human SMCs towards endothelial lineage through an intermediate vascular progenitor state induced by reprogramming.
Protein-protein binding selectivity and network topology constrain global and local properties of interface binding networks
Authors: Holland David O. , Shapiro Benjamin H. , Xue Pei , Johnson Margaret E.
Published: July 2017

Protein-protein interactions networks (PPINs) are known to share a highly conserved structure across all organisms. What is poorly understood, however, is the structure of the child interface interaction networks (IINs), which map the binding sites proteins use for each interaction. In this study we analyze four independently constructed IINs from yeast and humans and find a conserved structure of these networks with a unique topology distinct from the parent PPIN. Using an IIN sampling algorithm and a fitness function trained on the manually curated PPINs, we show that IIN topology can be mostly explained as a balance between limits on interface diversity and a need for physico-chemical binding complementarity. This complementarity must be optimized both for functional interactions and against mis-interactions, and this selectivity is encoded in the IIN motifs. To test whether the parent PPIN shapes IINs, we compared optimal IINs in biological PPINs versus random PPINs. We found that the hubs in biological networks allow for selective binding with minimal interfaces, suggesting that binding specificity is an additional pressure for a scale-free-like PPIN. We confirm through phylogenetic analysis that hub interfaces are strongly conserved and rewiring of interactions between proteins involved in endocytosis preserves interface binding selectivity.
Photonic Needles for Light Delivery in Deep Tissue-like Media
Authors: Fain Romy , Barbosa Felippe , Cardenas Jaime , Lipson Michal
Published: July 2017

We demonstrate a new platform for minimally invasive, light delivery probes leveraging the maturing field of silicon photonics, enabling massively parallel fabrication of photonic structures. These Photonic Needles probes have sub-10 μm cross-sectional dimensions, lengths greater than 3 mm–surpassing 1000 to 1 aspect ratio, and are released completely into air without a substrate below. We show the Photonic Needles to be mechanically robust when inserted into 2% agarose. The propagation loss of these waveguides is low–on the order of 4 dB/cm.
Common variants of T-cells contribute differently to phenotypic variation in sarcoidosis
Authors: Rivera Natalia V. , Hagemann-Jensen Michael , Ferreira Manuel A. R. , Kullberg Susanna , Eklund Anders , Martin Nicholas G. , Padyukov Leonid , Grunewald Johan
Published: July 2017

The involvement of the immune system, particularly the role of T-cells, in sarcoidosis is unclear. The existence of higher CD4+ T-cells and increased CD4/CD8 ratio may indicate a pathogenic role of T-cells in the disease. In this study, we quantified the contribution of T-cells associated variants and of CD4/CD8 ratio in sarcoidosis phenotypes, Löfgren’s syndrome (LS) and non- Löfgren’s syndrome (non-LS). We employed a polygenic-based approach using genome-wide association studies results on relative levels of T-cells in healthy individuals to measure the genetic contribution of T-cells in sarcoidosis entities. Results revealed that the genetic architecture of LS is highly influenced by genetic variants associated with CD8+ T-cells and CD4/CD8 ratio, explaining up to 7.94% and 6.49% of LS variation, respectively; whereas, the genetic architecture of non-LS is minimally influenced by T-cells, explaining a phenotypic variation of <1%. Moreover, pleiotropy assessment between T-cells and LS/non-LS associated-variants led to the discovery of highly scored pathway maps that shared common factors related to antigen presentation and T-cell regulatory mechanisms. Differences in significant polygenic scores, presence of pleiotropy, and distinct genetic factors provide further insights on how genetic variants and genes associated with relative levels of T-cell subtypes contribute differently to sarcoidosis phenotypes.
An Amino Acid Signature Associated with Obesity Predicts 2-Year Risk of Hypertriglyceridemia in School-Age Children
Authors: Moran-Ramos Sofia , Ocampo-Medina Elvira , Gutierrez-Aguilar Ruth , Macías-Kauffer Luis , Villamil-Ramírez Hugo , López-Contreras Blanca E. , León-Mimila Paola , Vega-Badillo Joel , Gutierrez-Vidal Roxana , Villarruel-Vazquez Ricardo , Serrano-Carbajal Erandi , Del-Río-Navarro Blanca E , Huertas-Vázquez Adriana , Villarreal-Molina Teresa , Ibarra-Gonzalez Isabel , Vela-Amieva Marcela , Aguilar-Salinas Carlos A. , Canizales-Quinteros Samuel
Published: July 2017

Childhood obesity is associated with a number of metabolic abnormalities leading to increased cardiovascular risk. Metabolites can be useful as early biomarkers and new targets to promote early intervention beginning in school age. Thus, we aimed to identify metabolomic profiles associated with obesity and obesity-related metabolic traits. We used data from the Obesity Research Study for Mexican children (ORSMEC) in Mexico City and included a case control (n = 1120), cross-sectional (n = 554) and a longitudinal study (n = 301) of 6–12-year-old children. Forty-two metabolites were measured using electrospray MS/MS and multivariate regression models were used to test associations of metabolomic profiles with anthropometric, clinical and biochemical parameters. Principal component analysis showed a serum amino acid signature composed of arginine, leucine/isoleucine, phenylalanine, tyrosine, valine and proline significantly associated with obesity (OR = 1.57; 95%CI 1.45–1.69,P = 3.84 × 10−31) and serum triglycerides (TG) (β = 0.067,P = 4.5 × 10−21). These associations were validated in the cross-sectional study (P < 0.0001). In the longitudinal cohort, the amino acid signature was associated with serum TG and with the risk of hypertriglyceridemia after 2 years (OR = 1.19; 95%CI 1.03-1.39,P = 0.016). This study shows that an amino acid signature significantly associated with childhood obesity, is an independent risk factor of future hypertriglyceridemia in children.
Fish oil supplementation to rats fed high-fat diet during pregnancy prevents development of impaired insulin sensitivity in male adult offspring
Authors: Albert Benjamin B. , Vickers Mark H. , Gray Clint , Reynolds Clare M. , Segovia Stephanie A. , Derraik José G. B. , Garg Manohar L. , Cameron-Smith David , Hofman Paul L. , Cutfield Wayne S.
Published: July 2017

We examined whether maternal fish oil supplementation during pregnancy could prevent development of insulin resistance in adult male offspring of rat dams fed a high-fat diet. Time-mated Sprague-Dawley rat dams were randomised into four treatment groups: Con-Con, dams fed a control diet (fat: 15% kcal) and administered water by gavage; Con-FO, control diet with unoxidised fish oil by gavage; HF-Con, high-fat diet (fat: 45% kcal) and water by gavage; and HF-FO, high-fat diet and unoxidised fish oil by gavage. Dams were fed the allocated diet ad libitum during pregnancy and lactation, but daily gavage occurred only during pregnancy. After weaning, male offspring consumed a chow diet ad libitum until adulthood. Maternal high-fat diet led to increased food consumption, adiposity, systolic blood pressure, and triglycerides and plasma leptin in adult HF-Con offspring. HF-Con offspring also exhibited lower insulin sensitivity than Con-Con rats. Male offspring from HF-FO group were similar to HF-Con regarding food consumption and most metabolic parameters. However, insulin sensitivity in the HF-FO group was improved relative to the HF-Con offspring. Supplementation with unoxidised n-3 PUFA rich oils in the setting of a maternal obesogenic diet improved insulin sensitivity, but had no impact on body composition of adult male offspring.
RADseq provides evidence for parallel ecotypic divergence in the autotetraploid Cochlearia officinalis in Northern Norway
Authors: Brandrud Marie K. , Paun Ovidiu , Lorenzo Maria T. , Nordal Inger , Brysting Anne K.
Published: July 2017

Speciation encompasses a continuum over time from freely interbreeding populations to reproductively isolated species. Along this process, ecotypes – the result of local adaptation – may be on the road to new species. We investigated whether three autotetraploidCochlearia officinalisecotypes, adapted to different habitats (beach, estuary, spring), are genetically differentiated and result from parallel ecotypic divergence in two distinct geographical regions. We obtained genetic data from thousands of single nucleotide polymorphisms (SNPs) from restriction-site associated DNA sequencing (RADseq) and from six microsatellite markers for 12 populations to assess genetic divergence at ecotypic, geographic and population level. The genetic patterns support differentiation among ecotypes as suggested by morphology and ecology. The data fit a scenario where the ancestral beach ecotype has recurrently and polytopically given rise to the estuary and spring ecotypes. Several ecologically-relevant loci with consistent non-random segregating patterns are identified across the recurrent origins, in particular around genes related to salt stress. Despite being ecologically distinct, theCochleariaecotypes still represent an early stage in the process of speciation, as reproductive isolation has not (yet) developed. A sequenced annotated genome is needed to specifically target candidate genes underlying local adaptation.
A Novel Pathogen Capturing Device for Removal and Detection
Authors: Kim Gwangseong , Vinerean Horatiu , Gaitas Angelo
Published: July 2017

A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80.3 ± 5.6% of Staphylococcus aureus with a starting concentration of ~107 CFU/mL and 95.4 ± 1.0% of Methicillin-resistant Staphylococcus aureus with starting concentration of ~104 CFU/mL were removed from 5 mL blood in a few hours. This concept was extended to live rats with an induced bloodstream S. aureus infection. A reduction of two orders of magnitude in the bacterial load of the rats was observed within a few hours. The same technique was used to capture a food pathogen, Salmonella typhimurium, with starting concentrations as low as ~100 CFU, from 100 or 250 mL of culture broth within similar timeframes as above. The feasibility for food pathogen testing applications was additionally confirmed by capturing and detecting S. typhimurium in ground chicken and ground beef.