bluuebunny/crossref_metadata_2025 huggingface dataset:
Rapid PRRSV-2 ORF5-based lineage classification using Nextclade
Authors: Zeller Michael A. , Chang Jennifer , Trevisan Giovani , Main Rodger G. , Gauger Phillip C. , Zhang Jianqiang
Published: September 2024

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a global challenge for swine health. Yim-Im et al. 2023 provides a standard genetic nomenclature, extending previously published works to better characterize PRRSV-2 ORF5-based genetic lineages on a global scale. To facilitate the use of this nomenclature, scaffold sequences, including historical and contemporary vaccines, were synthesized into a dataset designed for Nextclade v3.0. Metadata from the scaffold sequences representing year, country, and RFLP typing of the sequence were incorporated into the dataset. These scaffold sequences were processed through the Augur pipeline using DQ478308.1 as a reference strain for rooting and comparison. The resultant classifier can be accessed through the Nextclade website (https://clades.nextstrain.org/) or a link on the PRRSView homepage (https://prrsv.vdl.iastate.edu/). The resultant classifier functions the same as other classifiers hosted by the Nextclade core group and can provide phylogenetic-based PRRSV-2 ORF5 classifications on demand. Nextclade provides additional sequence metrics such as classification quality and notable mutations relative to the reference. The submitted sequences are grafted to the reference tree using phylogenetic placement, allowing for comparison to nearby sequences of reference viruses and vaccine strains. Additional comparisons between sequences can be made with metadata incorporated in the dataset. Although Nextclade is hosted as a webtool, the sequences are not uploaded to a server, and all analysis stay strictly confidential to the user. This work provides a standardized, trivial workflow facilitated by Nextclade to rapidly assign lineage classifications to PRRSV-2, identify mutations of interest, and compare contemporary strains to relevant vaccines.
Longitudinal surveillance of Coxiella burnetii following an abortion storm in domestic goats
Authors: Miller Halie K. , Priestley Rachael A. , Smith Cody B. , Cherry Cara , Kersh Gilbert J.
Published: September 2024

Q fever is a disease caused by Coxiella burnetii, which can cause serious illness in humans and abortions in goats. A Q fever outbreak among an unvaccinated goat herd led to a 65% loss of the kid crop in spring 2018. To assess the impact of the outbreak on the herd and environment, longitudinal surveillance of the ranch was conducted across three samplings in September 2018, April 2019, and May 2022. Antibodies against C. burnetii were monitored by an indirect immunofluorescence assay. Shedding was monitored through analysis of vaginal/fecal swabs and milk. Environmental swabs and bulk soil were collected from various locations around the ranch. Animal and environmental samples were analyzed for C. burnetii DNA by PCR. Herd-level seroprevalence decreased from 89% in 2018 to 84.3% in 2019, and 64.5% in 2022. Overall herd shedding was 14.4% in 2018, 7.4% in 2019, and 6.7% in 2022. The percentage of C. burnetii-positive environmental samples was 83.7% in 2018, 51.7% in 2019, and 28.6% in 2022. Serological evidence suggests that new infections were occurring in the herd 4 years post-abortion storm. This study demonstrates the presence of C. burnetii shedding and environmental contamination in a goat operation at least four kidding seasons after an outbreak. A better understanding of management practices that can improve outcomes for infected herds, particularly in areas without access to vaccines against C. burnetii, is needed to better protect operators and the public.
Effects of Fuel Penetration on the RDE Performance with JISC Injector Configuration
Authors: Mo Gyeong-Ui , Koo In-Hoi , Lee Keon-Hyeong , Choi Su-Wan , Choi Jeong-Yeol
Published: September 2024

This study investigates the operational characteristics of the Rotating Detonation Engine (RDE), with a focus on fuel injector design. Inspired by the similarity between the fuel injection structure of RDE and the Jet in Supersonic Crossflow (JISC) of a scramjet, experimental research on fuel injectors with jet penetration was conducted. Five injectors were designed, each with a fixed fuel injection area or injection hole diameter. Experiments determined practical injection areas, and an empirical correlation was used to calculate jet penetration heights. Under conditions of a total mass flow rate of 105 ± 5 g/s and an equivalence ratio of 1.05 ± 0.1, combustion modes were analyzed. Initial detonation occurrence was assessed through pressure history, with a detailed analysis via image post-processing. The results indicated that the injector D4N15, with the highest jet penetration height, exhibited deflagration, while D4N23 showed chaotic propagation. The injector D2N60 demonstrated relatively unstable behavior in sustained detonation cases. Thrust comparisons revealed that D4N30, with wider hole spacing and higher jet penetration height, exhibited approximately 12.5% higher specific impulse compared to D1N240. These outcomes confirm the significant impact of jet penetration height and hole spacing on detonation propagation and engine performance.
Simulation Analysis and Test of a Cleaning Device for a Fresh-Peanut-Picking Combine Harvester Based on Computational Fluid Dynamics–Discrete Element Method Coupling
Authors: Ling Jie , Gu Man , Luo Weiwen , Shen Haiyang , Hu Zhichao , Gu Fengwei , Wu Feng , Zhang Peng , Xu Hongbo
Published: September 2024

In order to solve the problems of high impurity rate and large loss rate in the whole fresh peanut harvesting and production process in hilly areas of China, the method of computational fluid dynamics (CFD) and discrete element (DEM) coupling is used to examine the gas–solid two-phase simulation of the cleaning device of the crawler fresh-peanut-picking combine harvester. In addition, the Box–Behnken test method is used to analyze the influence of different parameters on the impurity content and loss rate of materials in the cleaning process by taking the fan speed, feed amount, and air inlet angle as the test factors, and the optimal combination of working parameters is sought. The simulation results show that when the fan speed is 2905.07 r/min, the feed rate is 0.80 kg/s, the air inlet angle is 43.14°, the impurity content is 7.32%, and the loss rate is 4.78%. Compared with the simulation test results, the impurity content is increased by 0.68%, and the loss rate is increased by 1.24%, which verifies the reliability of the simulation model, and the research results provide some technical support for the improvement of the cleaning device in the later stages.
Impact of Linking Livelihood Resilience of Smallholder Households and the Risk Management Strategies: The Case of China from Socioeconomic Perspectives
Authors: Liu Xinming , Song Zhe , Xu Jie , Feng Weilin , Liu Wei
Published: September 2024

The government of China has implemented the Southern Shaanxi Disaster Resettlement program since 2011, which aims to address the problems of reduced livelihood resilience, increased livelihood risks, and single-risk management strategies caused by the frequent occurrence of natural disasters. This study considers the specific situation of disaster resettlement in Ankang Prefecture, southern Shaanxi Province, and draws on Quandt’s measurement idea to quantify livelihood resilience at the household scale in terms of five types of capital assets: natural, physical, human, financial, and social. A coarsened exact matching model was used to control confounding factors in the observational data to reduce sample selection bias, and then multinomial logit regression models were used to examine how livelihood resilience affects risk management strategies; moreover, the effects of different indicators of livelihood resilience, relocation characteristics, and follow-up support measures on risk management strategies were analyzed. Results show that livelihood resilience is higher among new-stage relocation, voluntary relocation, and centralized resettlement households, and working outside of the home accounts for the largest proportion of risk management strategies chosen by the sample households. In addition, livelihood resilience and its dimensions and indicators, relocation characteristics, and follow-up support measures have different impacts on risk management strategies. These results have considerable significance in guiding research on risk management strategies at the household scale and can serve as a reference for disaster resettlement in other developing nations and regions.
The Effect of Drip Irrigation and Nitrogen Levels on the Oil and Fatty Acid Composition of Sesame and Its Economic Analysis
Authors: Tas Ismail , Akcura Sevim , Kaplan Mahmut , Jagosz Barbara , Atılgan Atılgan , Kocięcka Joanna , Rolbiecki Roman , Liberacki Daniel , Rolbiecki Stanisław
Published: September 2024

One of the oldest oilseed crops is sesame, which is mainly cultivated due to its valuable oleic/linolenic fatty acid ratio. The application of precise irrigation and fertilisation is crucial to ensure the continuity and productivity of sesame production, especially in arid and semi-arid regions. This study aimed to determine the effect of drip irrigation and nitrogen levels on sesame’s oil and fatty acid composition. For this purpose, four nitrogen doses (N0: 0 kg ha−1, N30: 30 kg ha−1, N60: 60 kg ha−1 and N90: 90 kg ha−1) and three different irrigation water levels (I50, I75 and I100, which correspond to 50, 75 and 100% evaporation levels from the evaporation of the Class A pan) were applied. The highest oleic acid content (43.06%) was obtained for the I75N90 treatment. In the case of linoleic fatty acid, the greatest value (43.66%) was for I50N0 treatment. The effects of irrigation and nitrogen doses on oleic acid and linoleic acid content were inverse of each other. An increase in applied irrigation water increased the linoleic acid content. However, it caused a decrease in oleic acid content. Increasing the nitrogen dose increased the oleic acid content and caused a decrease in linoleic acid content. Furthermore, this study showed that the I50N60 treatment (50% Epan and a rate of 60 kg N ha−1) is the most effective for achieving high grain and oil yields in sesame cultivation. The results obtained provide practical guidance for farmers in sesame cultivation.
Characterization of Inductive Moderate Hyperthermia Effects on Intratumor Sarcoma-45 Heterogeneity Using Magnetic Resonance, Ultrasound and Histology Image Analysis
Authors: Orel Valerii B. , Dasyukevich Olga Yo. , Orel Valerii E. , Rykhalskyi Oleksandr Yu. , Kovalevska Larysa M. , Galkin Olexander Yu. , Matveichuk Karyna S. , Diedkov Anatolii G. , Ostafiichuk Vasyl V. , Shablii Oleksandr S.
Published: September 2024

Evaluating intratumor heterogeneity with image texture analysis offers a more sophisticated understanding of sarcoma response to treatment. We examined the effects of inductive moderate hyperthermia (IMH) on sarcoma-45 growth and intratumor heterogeneity across tissue, cellular and molecular levels using magnetic resonance imaging (MRI), ultrasound and histology image analysis. IMH (42 MHz, 20 W) inhibited sarcoma-45 growth kinetics by 34% compared to the untreated control group. T2-weighted MRI brightness was increased by 42%, reflecting more extensive tumor necrosis, while Young’s modulus increased by 37% due to more pronounced connective tissue replacement in response to IMH. Whereas calculations of Moran’s spatial autocorrelation index revealed distinctions in heterogeneity between tumor core, periphery and capsule regions of interest (ROIs) on MRI, ultrasound and histological examination in the untreated tumor-bearing animals, there was no significant difference between core and periphery after IMH. Exposure to IMH increased overall tumor ROI heterogeneity by 22% on MRI but reduced heterogeneity in the core and periphery on ultrasound and histology images. Ki-67 protein distribution was 25% less heterogeneous on the tumor periphery after IMH. Therefore, this study provides a quantitative characterization of IMH effects on different manifestations of intratumor sarcoma-45 heterogeneity using experimental imaging data.
A Study on Karst Cave Collapse Based on Improved Terzaghi Theory and Upper Limit Analysis
Authors: Yan Weilong , Liu Rui , Tian Shugao , Tan Fei , Wen Hao , Lv Jiahe
Published: September 2024

Karst areas exhibit intricate geological attributes, and the geological and environmental issues caused by urban development cannot be ignored, especially the issue of karst surface collapses. In this study, we developed two analytical methods and analyzed the stability of the overburden stratum of 3D spherical karst caves with surface load, vacuum absorption erosion force, and groundwater table considerations. The first analytical method is based on the improved Terzaghi theory, while the second analytical method is based on the upper limit analysis. A case study was conducted in Wuhan, China. The results from both analytical methods indicated a potential susceptibility to collapse, suggesting the excellent accuracy of these two methods. The results were also compared with the numerical solutions from previous studies. Notably, the accuracy of the upper limit analysis was inversely proportional to the depth ratio, while the results obtained through the improved Terzaghi theory were consistent with those of the numerical solutions, particularly under conditions of relatively high depth ratios. This study examined various facets, including the development of karst caves, soil shear strength, groundwater table fluctuations, and boundary failure angles. Furthermore, we explored the effects of geometric and geotechnical parameters on the stability of karst caves.
Enhancing the Image Pre-Processing for Large Fleets Based on a Fuzzy Approach to Handle Multiple Resolutions
Authors: Mu Ching-Yun , Kung Pin
Published: September 2024

Image pre-processing is crucial for large fleet management. Many traffic videos are collected by closed-circuit television (CCTV), which has a fixed area monitoring for image analysis. This paper adopts the front camera installed in large vehicles to obtain moving traffic images, whereas CCTV is more limited. In practice, fleets often install cameras with different resolutions due to cost considerations. The cameras evaluate the front images with traffic lights. This paper proposes fuzzy enhancement with RGB and CIELAB conversions to handle multiple resolutions. This study provided image pre-processing adjustment comparisons, enabling further model training and analysis. This paper proposed fuzzy enhancement to deal with multiple resolutions. The fuzzy enhancement and fuzzy with brightness adjustment produced images with lower MSE and higher PSNR for the images of the front view. Fuzzy enhancement can also be used to enhance traffic light image adjustments. Moreover, this study employed You Only Look Once Version 9 (YOLOv9) for model training. YOLOv9 with fuzzy enhancement obtained better detection performance. This fuzzy enhancement made more flexible adjustments for pre-processing tasks and provided guidance for fleet managers to perform consistent image-enhancement adjustments for handling multiple resolutions.
Exploring Marine-Based Food Production: The Challenges for a Sustainable and Fast Biotechnology-Based Development
Authors: Augusto Ana , Lemos Marco , Silva Susana
Published: September 2024

Marine-derived nutrients and bioactive compounds may offer a myriad of biological benefits, such as anticancer and anti-inflammatory properties, and technological potential, enhancing food quality as additives. Their role in the sustainable development of food technology is fundamental, especially in advancing the knowledge of functional foods and related technologies. Algae are considered one of the major sources of marine-derived ingredients and the subject of several recent studies. Despite their potential, the translation of marine ingredients’ potential into a marine-based competitiveness of the food industry faces hurdles in the extraction process and operational systems scale-up that the industry needs to tackle. The complexity of marine matrices with diverse compounds and solubilities adds complexity to extraction processes and may lead to low yields or bioactivity loss. Contaminants, like heavy metals and pesticide residues in marine organisms, require rigorous purification processes for product safety. The use of biorefinery systems in marine-based ingredients’ production, particularly cascade processes, offers zero-waste solutions, contributing to the blue economy and aligning with UN sustainability goals. Sustainability assessment tools are critical for evaluating marine-based food production’s environmental, social, and economic impacts. A continued exploration and collaboration are essential for the future, fostering innovation and sustainability to create a resilient, equitable, and eco-friendly food system.
Enhanced Water Quality Inversion in the Ningxia Yellow River Basin Using a Hybrid PCWA-ResCNN Model: Insights from Landsat-8 Data
Authors: Li Qi , Guo Zhonghua , Li Jialong , Li Xiaojun , Ban Bo
Published: September 2024

The real-time monitoring and evaluation of water quality provides a scientific basis for water resource management and promotes regional sustainable development. This study established a database using Landsat-8 satellite data and water quality data from the Ningxia Yellow River basin in China, spanning 2021 to 2023, and this paper proposes a custom residual convolutional neural network model with a hybrid attention mechanism, referred to as PCWA-ResCNN. The accuracy of the model in predicting turbidity, permanganate, ammonia nitrogen, and dissolved oxygen concentration was more than 95%. Compared to convolutional neural networks and long short-term memory models, this model performed better in predicting water quality parameters with significantly improved prediction performance. In terms of spatial distribution, the pollution degree in the middle reaches of the basin is relatively serious. However, the overall water quality is good, being mainly Class I and Class II water quality. The hybrid model established in this paper can better capture the complex nonlinear relationship between the observed values and the surface water reflectance, showing strong robustness. This model can be used for the water quality monitoring of complex inland rivers and lakes, and it can also provide effective support for relevant government departments to formulate scientific and reasonable water quality management policies.
Comprehensive Insights into the Molecular Basis of HIV Glycoproteins
Authors: Elalouf Amir , Maoz Hanan , Rosenfeld Amit Yaniv
Published: September 2024

Human Immunodeficiency Virus (HIV) is a diploid, C-type enveloped retrovirus belonging to the Lentivirus genus, characterized by two positive-sense single-stranded RNA genomes, that transitioned from non-human primates to humans and has become globally widespread. In its advanced stages, HIV leads to Acquired Immune Deficiency Syndrome (AIDS), which severely weakens the immune system by depleting CD4+ helper T cells. Without treatment, HIV progressively impairs immune function, making the body susceptible to various opportunistic infections and complications, including cardiovascular, respiratory, and neurological issues, as well as secondary cancers. The envelope glycoprotein complex (Env), composed of gp120 and gp41 subunits derived from the precursor gp160, plays a central role in cycle entry. gp160, synthesized in the rough endoplasmic reticulum, undergoes glycosylation and proteolytic cleavage, forming a trimeric spike on the virion surface. These structural features, including the transmembrane domain (TMD), membrane-proximal external region (MPER), and cytoplasmic tail (CT), are critical for viral infectivity and immune evasion. Glycosylation and proteolytic processing, especially by furin, are essential for Env’s fusogenic activity and capacity to evade immune detection. The virus’s outer envelope glycoprotein, gp120, interacts with host cell CD4 receptors. This interaction, along with the involvement of coreceptors CXCR4 and CCR5, prompts the exposure of the gp41 fusogenic components, enabling the fusion of viral and host cell membranes. While this is the predominant pathway for viral entry, alternative mechanisms involving receptors such as C-type lectin and mannose receptors have been found. This review aims to provide an in-depth analysis of the structural features and functional roles of HIV entry proteins, particularly gp120 and gp41, in the viral entry process. By examining these proteins’ architecture, the review elucidates how their structural properties facilitate HIV invasion of host cells. It also explores the synthesis, trafficking, and structural characteristics of Env/gp160 proteins, highlighting the interactions between gp120, gp41, and the viral matrix. These contributions advance drug resistance management and vaccine development efforts.
WorkloadGPT: A Large Language Model Approach to Real-Time Detection of Pilot Workload
Authors: Gao Yijing , Yue Lishengsa , Sun Jiahang , Shan Xiaonian , Liu Yihan , Wu Xuerui
Published: September 2024

The occurrence of flight risks and accidents is closely related to pilot workload. Effective detection of pilot workload has been a key research area in the aviation industry. However, traditional methods for detecting pilot workload have several shortcomings: firstly, the collection of metrics via contact-based devices can interfere with pilots; secondly, real-time detection of pilot workload is challenging, making it difficult to capture sudden increases in workload; thirdly, the detection accuracy of these models is limited; fourthly, the models lack cross-pilot generalization. To address these challenges, this study proposes a large language model, WorkloadGPT, which utilizes low-interference indicators: eye movement and seat pressure. Specifically, features are extracted in 10 s time windows and input into WorkloadGPT for classification into low, medium, and high workload categories. Additionally, this article presents the design of an appropriate text template to serialize the tabular feature dataset into natural language, incorporating individual difference prompts during instance construction to enhance cross-pilot generalization. Finally, the LoRA algorithm was used to fine-tune the pre-trained large language model ChatGLM3-6B, resulting in WorkloadGPT. During the training process of WorkloadGPT, the GAN-Ensemble algorithm was employed to augment the experimental raw data, constructing a realistic and robust extended dataset for model training. The results show that WorkloadGPT achieved a classification accuracy of 87.3%, with a cross-pilot standard deviation of only 2.1% and a response time of just 1.76 s, overall outperforming existing studies in terms of accuracy, real-time performance, and cross-pilot generalization capability, thereby providing a solid foundation for enhancing flight safety.
A Novel Tracking Strategy Based on Real-Time Monitoring to Increase the Lifetime of Dual-Axis Solar Tracking Systems
Authors: Flores-Hernández Diego A. , Islas-Estrada Luis R. , Palomino-Resendiz Sergio I.
Published: September 2024

Solar tracking systems allow an increase in the use of solar energy for its conversion with photovoltaic technology due to the alignment with the sun. However, there is a compromise between tracking accuracy and the energy required to perform the movement action. Consequently, the wear of the tracker components increases, reducing its useful lifetime and affecting the profitability of these systems. The present research develops a novel tracking strategy based on real-time measurements to increase the lifetime without reducing the energy productivity of the tracking systems. The proposed approach is verified experimentally by implementing the real-time decision-making algorithm and a conventional tracking algorithm in identical tracking systems under the same weather conditions. The proposed strategy reduces energy consumption by 14.18% due to the tracking action, maintaining a practically identical energy generation between both systems. The findings highlight a 53.33% reduction in the movements required for tracking and a 60.77% reduction in operation time, which translates into a 6.8-fold increase in the lifetime of the solar tracking system under the experimental conditions applied. The results are promising, so this research initiates and motivates the development of more complex models to increase the useful life of the tracking systems and their profitability and environmental impact concurrently.
I-Convergence Sequence Paranormed Spaces of Order (α, β)
Authors: Su Lian-Ta , Kumar Ravi , Sharma Sunil K. , Sharma Ajay K. , Cai Qing-Bo
Published: September 2024

In this paper, we introduce and rigorously define a novel class of difference sequence spaces, denoted by wI(M,∆vu,r)αβ, w0I(M,∆vu,r)αβ, w∞I(M,∆vu,r)αβ, and w∞(M,∆vu,r)αβ. These spaces are constructed through the application of the concept of I-convergence of sequences, combined with a Musielak–Orlicz function of order (α, β). The primary focus of our work is to thoroughly investigate the algebraic and topological properties of these defined sequence spaces. We explore their linearity, examine their structure within the framework of paranormed spaces, and analyze various other algebraic characteristics pertinent to these spaces. In addition, we examine the topological nature of these sequence spaces, identifying the conditions under which they exhibit specific topological properties. A significant part of our study is dedicated to examining the inclusion relationships between these sequence spaces, thereby providing a comprehensive understanding of how these spaces are interrelated. Our analysis contributes to the broader field of functional analysis and sequence space theory, offering new insights and potential applications of these advanced mathematical constructs.
Artificial Intelligence and Machine Learning in Spine Research: A New Frontier
Authors: Chang Min Cheol
Published: September 2024

Artificial Intelligence (AI) refers to the creation of computer systems capable of performing tasks typically requiring human intelligence [...]
Optical Transmission in Single-Layer Brain Tissues under Different Optical Source Types: Modelling and Simulation
Authors: Yang Xi , Chai Chengpeng , Chen Yun-Hsuan , Sawan Mohamad
Published: September 2024

The human brain is a complex organ controlling daily activity. Present technique models have mostly focused on multi-layer brain tissues, which lack understanding of the propagation characteristics of various single brain tissues. To better understand the influence of different optical source types on individual brain tissues, we constructed single-layer brain models and simulated optical propagation using the Monte Carlo method. Based on the optical simulation results, sixteen optical source types had different optical energy distributions, and the distribution in cerebrospinal fluid had obvious characteristics. Five brain tissues (scalp, skull, cerebrospinal fluid, gray matter, and blood vessel) had the same set of the first three optical source types with maximum depth, while white matter had a different set of the first three optical source types with maximum depth. Each brain tissue had different optical source types with the maximum and minimum full width at half maximum. The study on single-layer brain tissues under different optical source types lays the foundation for constructing complex brain models with multiple tissue layers. It provides a theoretical reference for optimizing the selection of optical source devices for brain imaging.
Impact of Uniaxial Static Strain on Myoblast Differentiation in Collagen-Coated PCL Microfilament Scaffolds: Role of Onset Time of Mechanical Stimulation
Authors: Espinoza-Álvarez María Laura , Rojas-Rojas Laura , Morales-Sánchez Johan , Guillén-Girón Teodolito
Published: September 2024

Tissue engineering endeavors to create in vitro constructs that replicate the properties of native tissue, such as skeletal muscle. This study investigated the use of mechanical stimulation to promote myogenic differentiation and enhance the functionality of bioengineered tissues. Specifically, it aimed to facilitate the differentiation of myoblasts within a three-dimensional scaffold using a defined pattern of mechanical stimulation. C2C12 cells were cultured on a collagen-coated PCL microfilament scaffold and subjected to 24 h of uniaxial static strain using a biomechanical stimulation system. Two onset times of stimulation, 72 h and 120 h post-seeding, were evaluated. Cell proliferation, myogenic marker expression, and alterations in cell morphology and orientation were assessed. Results indicate that static strain on the scaffold promoted myoblast differentiation, evidenced by morphological and molecular changes. Notably, strain initiated at 72 h induced an early differentiation stage marked by MyoD expression, whereas stimulation beginning at 120 h led to a mid-stage differentiation characterized by the co-expression of MyoD and Myogenin, culminating in myotube formation. These results highlight the critical influence of myoblast maturity at the time of strain application on the differentiation outcome. This study provides insights that could guide the optimization of mechanical stimulation protocols in tissue engineering applications.
Chitosan-Modified AgNPs Efficiently Inhibit Swine Coronavirus-Induced Host Cell Infections via Targeting the Spike Protein
Authors: Wang Dongliang , Yin Caiyun , Bai Yihan , Zhou Mingxia , Wang Naidong , Tong Chunyi , Yang Yi , Liu Bin
Published: September 2024

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has filled a gap in our knowledge regarding the prevention of CoVs. Swine coronavirus (CoV) is a significant pathogen that causes huge economic losses to the global swine industry. Until now, anti-CoV prevention and control have been challenging due to the rapidly generated variants. Silver nanoparticles (AgNPs) with excellent antimicrobial activity have attracted great interest for biosafety prevention and control applications. In this study, we synthesized chitosan-modified AgNPs (Chi-AgNPs) with good biocompatibility to investigate their antiviral effects on swine CoVs. In vitro assays showed that Chi-AgNPs could significantly impaired viral entry. The direct interaction between Chi-AgNPs and CoVs can destroy the viral surface spike (S) protein secondary structure associated with viral membrane fusion, which is caused by the cleavage of disulfide bonds in the S protein. Moreover, the mechanism showed that Chi-AgNPs reduced the virus-induced apoptosis of Vero cells via the ROS/p53 signaling activation pathway. Our data suggest that Chi-AgNPs can serve as a preventive strategy for CoVs infection and provide a molecular basis for the viricidal effect of Chi-AgNPs on CoVs.
3D-Cultured MC3T3-E1-Derived Exosomes Promote Endothelial Cell Biological Function under the Effect of LIPUS
Authors: Liu Xiaohan , Cheng Rui , Cao Hongjuan , Wu Lin
Published: September 2024

Porous Ti-6Al-4V scaffold materials can be used to heal massive bone defects because they can provide space for vascularisation and bone formation. During new bone tissue development, rapid vascular ingrowth into scaffold materials is very important. Osteoblast-derived exosomes are capable of facilitating angiogenesis–osteogenesis coupling. Low-intensity pulsed ultrasound (LIPUS) is a physical therapy modality widely utilised in the field of bone regeneration and has been proven to enhance the production and functionality of exosomes on two-dimensional surfaces. The impact of LIPUS on exosomes derived from osteoblasts cultured in three dimensions remains to be elucidated. In this study, exosomes produced by osteoblasts on porous Ti-6Al-4V scaffold materials under LIPUS and non-ultrasound stimulated conditions were co-cultured with endothelial cells. The findings indicated that the exosomes were consistently and stably taken up by the endothelial cells. Compared to the non-ultrasound group, the LIPUS group facilitated endothelial cell proliferation and angiogenesis. After 24 h of co-culture, the migration ability of endothelial cells in the LIPUS group was 17.30% higher relative to the non-ultrasound group. LIPUS may represent a potentially viable strategy to promote the efficacy of osteoblast-derived exosomes to enhance the angiogenesis of porous Ti-6Al-4V scaffold materials.
Prognostic Values of Ferroptosis-Related Proteins ACSL4, SLC7A11, and CHAC1 in Cholangiocarcinoma
Authors: Amontailak Supakan , Titapun Attapol , Jusakul Apinya , Thanan Raynoo , Kimawaha Phongsaran , Jamnongkan Wassana , Thanee Malinee , Sirithawat Papitchaya , Techasen Anchalee
Published: September 2024

Background: The epithelial malignant tumor known as cholangiocarcinoma (CCA) is most commonly found in Southeast Asia, particularly in northeastern Thailand. Previous research has indicated that the overexpression of acyl-CoA synthetase long-chain family member 4 (ACSL4), solute carrier family 7 member 11 (SLC7A11), and ChaC glutathione-specific γ-glutamylcyclotransferase (CHAC1) as ferroptosis-related proteins is associated with poorer prognosis in several cancers. The role of these three proteins in CCA is still unclear. The present study aimed to investigate the expression levels of ACSL4, SLC7A11, and CHAC1, all potential ferroptosis biomarkers, in CCA. Methods: The ACSL4, SLC7A11, and CHAC1 protein expression levels in 137 CCA tissues were examined using immunohistochemistry, while 61 CCA serum samples were evaluated using indirect ELISA. The associations between the expression levels of ACSL4, SLC7A11, and CHAC1 and patient clinicopathological data were evaluated to determine the clinical significance of these proteins. Results: The expression levels of ACSL4, SLC7A11, and CHAC1 were assessed in CCA tissues. A significant association was observed between high ACSL4 levels and extrahepatic CCA, tumor growth type, and elevated alanine transferase (ALT). There was also a positive association between elevated SLC7A11 levels and tumor growth type. Additionally, the upregulation of CHAC1 was significantly associated with a shorter survival time in patients. High levels of ACSL4 and SLC7A11 in CCA sera were both significantly associated with advanced tumor stages and abnormal liver function test results, indicating that they could be used as a reliable prognostic biomarker panel in patients with CCA. Conclusions: The results of the present study demonstrated that the upregulation of ACSL4, SLC7A11, and CHAC1 could be used as a valuable biomarker panel for predicting prognosis parameters in CCA. Furthermore, ACSL4 and SLC7A11 could potentially serve as complementary markers for improving the accuracy of prognosis prediction when CCA sera is used. These less invasive biomarkers could facilitate effective treatment planning.
Potential of Resveratrol to Combine with Hydrogel for Photodynamic Therapy against Bacteria and Cancer—A Review
Authors: Law Siu Kan , Liu Cris Wai Ching , Tong Christy Wing Sum , Au Dawn Ching Tung
Published: September 2024

Bacterial infections and cancers are important issues in public health around the world. Currently, Western medicine is the most suitable approach when dealing with these issues. “Antibiotics” and “Corticosteroids” are the Western medicines used for bacterial infection. “Chemotherapy drugs”, “surgery”, and “radiotherapy” are common techniques used to treat cancer. These are conventional treatments with many side effects. PDT is a non-invasive and effective therapy for bacterial infection and cancer diseases. Methods: Nine electronic databases, namely WanFang Data, PubMed, Science Direct, Scopus, Web of Science, Springer Link, SciFinder, and China National Knowledge Infrastructure (CNKI), were searched to conduct this literature review, without any regard to language constraints. Studies focusing on the photodynamic actions of hydrogel and Resveratrol were included that evaluated the effect of PDT against bacteria and cancer. All eligible studies were analyzed and summarized in this review. Results: Resveratrol has antibacterial and anticancer effects. It can also act as PS in PDT or adjuvant but has some limitations. This is much better when combined with a hydrogel to enhance the effectiveness of PDT in the fight against bacteria and cancer. Conclusions: Resveratrol combined with hydrogel is possible for PDT treatment in bacteria and cancer. They are compatible and reinforce each other to increase the effectiveness of PDT. However, much more work is required, such as cytotoxicity safety assessments of the human body and further enhancing the effectiveness of PDT in different environments for future investigations.
Risk Assessment of Underground Tunnel Engineering Based on Pythagorean Fuzzy Sets and Bayesian Networks
Authors: Wang Zhenhua , Jiang Tiantian , Li Zhiyong
Published: September 2024

With the acceleration of urbanization, the importance of risk management in underground construction projects has become increasingly prominent. In the process of risk assessment for underground construction projects, the uncertainty of subjective factors from experts poses a significant challenge to the accuracy of assessment outcomes. This paper takes a section of the Nanchang Metro Line 2 as the research object, aiming to address the subjectivity issues in the risk assessment of underground construction projects and to enhance the scientific rigor and accuracy of the assessment. The study initially conducts a comprehensive identification and analysis of risk factors in underground engineering through a literature review and expert consultation method. Based on this, this paper introduces the theory of Pythagorean fuzzy sets to improve the Delphi method in order to reduce the impact of subjectivity in expert assessments. Furthermore, this paper constructs a Bayesian network model, incorporating risk factors into the network, and quantifies the construction risks through a probabilistic inference mechanism. The research findings indicate a total of 12 key risk factors that have been identified across four dimensions: geological and groundwater conditions, tunnel construction technical risks, construction management measures, and the surrounding environment. The Bayesian network assessment results indicate that the effectiveness of engineering quality management and the state of safety management at the construction site are the two most influential factors. Based on the assessment results, this paper further conducts a risk control analysis and proposes targeted risk management measures.
Fractal Behavior of Nanostructured Pt/TiO2 Catalysts: Synthesis, Characterization and Evaluation of Photocatalytic Hydrogen Generation
Authors: Vasile Anca , Dobrescu Gianina , Bratan Veronica , Teodorescu Mircea , Munteanu Cornel , Atkinson Irina , Negrila Catalin , Papa Florica , Balint Ioan
Published: September 2024

The fractal characterization of supported nanoparticles is a useful tool for obtaining structural and morphological information that strongly impacts catalytic properties. We have synthesized and characterized Pt supported on TiO2 nanostructures. Triblock copolymers with thermosensitive properties were used as templating agents during the synthesis process. In addition to the several techniques used for the characterization of the materials, we carried out fractal analysis. The prepared materials showed a reduction in the band gap of TiO2 from 3.44 to 3.01 eV. The extended absorption in the 500–700 nm regions is mostly attributed to the presence of supported Pt nanoparticles. The ability of the nanostructured Pt/TiO2 catalysts to generate H2 in an aqueous solution was evaluated. The test reaction was carried out in the presence of methanol, as a hole scavenger, under simulated solar light. Pt/TiO2-3TB shows the highest rate of H2 (4.17 mmol h−1 gcat−1) when compared to Pt/TiO2-0TB (3.65 mmol h−1 gcat−1) and Pt/TiO2-6TB (2.29 mmol h−1 gcat−1) during simulated solar light irradiation. Pt/TiO2-3TB exhibits a more structured organization (fractal dimensions of 1.65–1.74 nm at short scales, 1.27–1.30 nm at long scales) and a distinct fractal behavior. The generation of hydrogen via photocatalysis can be linked to the fractal characteristics.
Photocatalysis as an Alternative for the Remediation of Wastewater: A Scientometric Review
Authors: Moreno-Vargas Jhoan Mauricio , Echeverry-Cardona Laura Maria , Torres-Ceron Darwin Augusto , Amaya-Roncancio Sebastian , Restrepo-Parra Elisabeth , Castillo-Delgado Kevin Jair
Published: September 2024

The objective of this study is to map, describe, and identify “water treatment using catalysts and/or nanomaterials” and their derivable aspects. A comprehensive search was conducted in academic databases such as WoS and Scopus, following the PRISMA methodology, to identify relevant studies published between 2010 and 2024. Inclusion and exclusion criteria were applied to select articles that address both experimental and theoretical aspects of photocatalysis in wastewater treatment. The methodology is developed through exploratory data analysis and the use of the Tree of Science algorithm. The first results indicate the roots, in which it is possible to gain knowledge of the environment for the implementation of a photoreactor it uses as a photocatalyst agent. A total of 94 relevant articles were identified. The results show that most studies focus on the degradation of organic pollutants using TiO2 as a photocatalyst. Additionally, there has been a significant increase in the number of publications and citations in recent years, indicating growing interest in this field. Then, in the trunk, some more solid ideas in terms of basic concepts, techniques and possible variations for the application of knowledge and development of future research related to the initial topic are indicated. Finally, through the leaves, new modifications and combinations of the photocatalytic materials are obtained, in search of improving their performance in terms of reduction in water contaminants. From the above, centrality in photocatalysis is identified as an alternative for water remediation using different photocatalysts. It is concluded that the total citation network contains, within the most important nodes, articles of high interest in the community, such as those authored by Zhang, Xiaofei; Nezamzadeh-Ejhieh, Alireza; or Li, Jingyi, from countries in the Middle East and the Asian continent, justified not only by the research capabilities of these countries, but also by the needs and problems that these regions face in terms of water scarcity. Future work indicates the need for and interest in improving various characteristics such as photocatalytic performance, the number of cycles that the material supports, and its reduction capacity in the presence of high concentrations of contaminants, with the intention of maximizing the benefits of its applicability in water treatment.
A Virtual Reality Platform for Evaluating Deficits in Executive Functions in Deaf and Hard of Hearing Children—Relation to Daily Function and to Quality of Life
Authors: Hamed-Daher Shaima , Josman Naomi , Klinger Evelyne , Engel-Yeger Batya
Published: September 2024

Background: Childhood hearing loss is a common chronic condition that may have a broad impact on children’s communication and motor and cognitive development, resulting in functional challenges and decreased quality of life (QoL). Objectives: This pilot study aimed to compare executive functions (EFs) as expressed in daily life and QoL between deaf and hard-of-hearing (D/HH) children and children with typical hearing. Furthermore, we examined the relationship between EFs and QoL in D/HH children. Methods: The participants were 76 children aged 7–11 yr: 38 D/HH and 38 with typical hearing. Parents completed the Behavior Rating Inventory of Executive Function (BRIEF) and Pediatric Quality of Life Inventory (PedsQL), while the child performed a shopping task in the virtual action planning supermarket (VAP-S) to reflect the use of EFs in daily activity. Results: D/HH children showed significantly poorer EFs (as measured by BRIEF and VAP-S) and reduced QoL. Difficulties in EFs were correlated with lower QoL. BRIEF scores were significant predictors of QoL domains. Conclusions: Difficulties in EFs may characterize children with D/HH and reduce their QoL. Therefore, EFs should be screened and treated. VAP-S and BRIEF are feasible tools for evaluating EFs that reflect children’s challenges due to EF difficulties in real-life contexts.
Exploring the Efficacy of Combining Radiofrequency Thermal Ablation or Microwave Ablation with Vertebroplasty for Pain Control and Disease Management in Metastatic Bone Disease—A Systematic Review
Authors: Faiella Eliodoro , Vaccarino Federica , Pacella Giuseppina , Santucci Domiziana , Vergantino Elva , Bruno Amalia , Ragone Raffaele , Zobel Bruno Beomonte , Grasso Rosario Francesco
Published: September 2024

Background: Interventional radiology techniques have become pivotal in recent years in managing metastatic bone disease, which frequently results in skeletal complications such as fractures and severe pain. Thermoablative methods like radiofrequency ablation (RFA) and microwave ablation (MWA), when combined with vertebroplasty (VP), are proving increasingly beneficial for these patients. Methods: The search was independently conducted by two radiologists on MEDLINE databases, using specified strings up to April 2024. Methodological quality was assessed using PRISMA guidelines. Studies meeting inclusion criteria investigated thermoablation techniques (RFA and/or MWA) combined with VP, focusing on pain management and disease control outcomes in adults. Results: Among 147 results, 42 articles met the criteria, with varied prospective and retrospective designs and sample sizes averaging 49 patients, predominantly involving RFA (30 studies), MWA (11 studies), and one comparative study. Our review highlights significant pain reduction, effective local tumor control, and favorable safety of combined RFA or MWA with VP, supporting its potential in managing vertebral pathologies and warranting further clinical integration. Conclusions: The combined treatment of RFA/MWA with VP demonstrates significant pain reduction and local tumor control, with a rapid onset of analgesic effect. These findings support its crucial role in clinical practice for managing vertebral metastases.
Revalidation of the Arboreal Asian Snake Genera Gonyophis Boulenger, 1891; Rhynchophis Mocquard, 1897; and Rhadinophis Vogt, 1922, with Description of a New Genus and Tribe (Squamata: Serpentes: Colubridae)
Authors: Wallach Van , Midtgaard Rune , Hsiao Emma
Published: September 2024

Based on the latest molecular phylogenies of Gonyosoma sensu lato, which recovered five clades with robust support, we utilize morphological characters to demonstrate the distinctiveness of each clade, resulting in the resurrection of three genera (Gonyophis Boulenger, 1891; Rhynchophis Mocquard, 1897; and Rhadinophis Vogt, 1922) and a proposal of a new genus and a new tribe. A synopsis of the group, with descriptions and diagnoses, is provided for the five genera and eight species in addition to distribution maps and illustrations of the head of each taxon. An artificial key to the species and genera in the new tribe is presented in addition to the estimated origin times for each clade.