bluuebunny/crossref_metadata_2025 huggingface dataset:
Functional Evaluation of Embedded Modular Single-Branched Stent Graft: Application to Type B Aortic Dissection With Aberrant Right Subclavian Artery
Authors: Zhang Xuehuan , Chen Duanduan , Wu Mingwei , Dong Huiwu , Wan Zhengdong , Jia Heyue , Liang Shichao , Shao Jun , Zheng Jun , Xu Shangdong , Xiong Jiang , Guo Wei
Published: May 2022

Background

Endovascular repair of type B aortic dissection (TBAD) with aberrant right subclavian artery (ARSA) is challenging due to anatomical complexity. The embedded modular single-branched stent graft (EMSBSG) could solve this problem. However, the hemodynamic efficacy of this innovative technique has not been fully assessed. This study aimed to propose morphometric and functional indicators to quantify the outcomes of EMSBSG in treating TBAD with ARSA.

Material and Methods

A patient who had TBAD with ARSA underwent EMSBSG implantation was admitted. Computational fluid dynamics (CFD) and three-dimensional structural analyses were conducted based on CTA datasets before the operation (Pre-1) and at 4 and 25 days after EMSBSG implantation (Post-1 and Post-2). Quantitative and qualitative functional analyses were conducted via pressure-, velocity- and wall shear stress (WSS) -based parameters, such as the luminal pressure difference (LPD), total energy loss, and flow distribution ratio. By precisely registering the aortas at the three time points, parameter variations in the EMSBSG region were also computed to investigate the prognostic improvement after EMSBSG implantation.

Results

The first balance point of LPD distally shifted to the abdominal aorta in Post-1 by a distance of 20.172 cm, and shifted out of the dissected region in Post-2, indicating positive pressure recovery post EMSBSG. The flow distribution ratios of all aortic arch branches increased after EMSBSG implantation. A positive normal deformation index in the EMSBSG region confirmed true lumen expansion; dominant ARN (area ratio of negative value) of pressure and WSS-based parameters indicated an improved prognosis from Post-1 to Post-2.

Conclusions

The short-term results of EMSBSG in treating TBAD with ARSA proved to be promising, especially in EMSBSG region. Comprehensive evaluation could provide new insight into the therapy of TBAD with ARSA. Thus, it might guide the further management of complex aortic arch lesions.
Multidisciplinary Effort to Drive Precision-Medicine for the Future
Authors: Kong Dewei , Yu Haojie , Sim Xueling , White Kevin , Tai E. Shyong , Wenk Markus , Teo Adrian Kee Keong
Published: May 2022

In the past one or two decades, countries across the world have successively implemented different precision medicine (PM) programs, and also cooperated to implement international PM programs. We are now in the era of PM. Singapore's National Precision Medicine (NPM) program, initiated in 2017, is now entering its second phase to generate a large genomic database for Asians. The National University of Singapore (NUS) also launched its own PM translational research program (TRP) in 2021, aimed at consolidating multidisciplinary expertise within the Yong Loo Lin School of Medicine to develop collaborative projects that can help to identify and validate novel therapeutic targets for the realization of PM. To achieve this, appropriate data collection, data processing, and results interpretation must be taken into consideration. There may be some difficulties during these processes, but with the improvement of relevant rules and the continuous development of omics-based technologies, we will be able to solve these problems, eventually achieving precise prediction, diagnosis, treatment, or even prevention of diseases.
Spatiotemporal Impact on Vegetation by Tunnel Discharging in the Jinping II Hydropower Station: Evidence From the NDVI and GPP Monitoring Dataset of 2001–2019
Authors: Song Ci , Huang Runqiu , Tang Xiaolu
Published: May 2022

An impact on vegetation by tunnel discharging has been a critical point for underground constructions around the world. The Jinping II hydropower station in China possesses the world’s largest karst diversion tunnel group and discharged massive groundwater during the construction period. This study assessed the effects on vegetation in the karst and non-karst areas at different stages before construction (2001–2003, BC), during construction (2004–2014, DC), and after construction (2015–2019, AC) based on the Normalized Difference Vegetation Index (NDVI) and the gross primary productivity (GPP) dataset of the moderate resolution-imaging spectroradiometer (MODIS). The regional median results of the NDVI and GPP showed that the whole vegetation was little changed by massive tunnel drainage throughout BC, DC, and AC. The NDVI and GPP were locally decreased in the first 3 years, and then recovered to the original level within next several years. Notably, the NDVI and GPP of the karst area (20 and 50% in the total area) were decreased more significantly than those of the non-karst area (8 and 20% in the total area). The factors controlling vegetation change are the development of karst and fractures, which are the conduits of groundwater flow. Therefore, massive groundwater discharge is the primary influence leading to the vegetation deterioration in the Jinping II hydropower station. The research would provide a vital reference for vegetation protection of large-scale underground construction in the world.
Mechanical Characteristics of Heishan Bentonite for Hazardous Waste Contamination Prevention
Authors: Xu Jian-Hua , Li Kai , Sun Ke-Ming , Zhang Shu-Cui
Published: May 2022

A series of laboratory-based water infiltration tests at room temperature have been conducted to investigate the mechanical characteristics of Heishan bentonite as a potential backfill material. The experiments were performed with an oedometer testing apparatus which facilitates the mechanical loading, the water infiltration, and the deformation measurement. The results show that both swelling pressure-initial dry density and swelling strain-moisture content have an exponential relationship. Moreover, swelling strain dramatically decreases when the applied stress exceeds a reference stress of 100 kpa. Finally, permeability tests were carried on Heishan bentonite specimen and the studied material reaches an impermeable state when the moisture content is larger than 40%. The presented properties certificate that Heishan bentonite can be employed as a backfill material in engineering practice.
Influence of Maternal Age on the Relationship Between Endometrial Thickness and Ongoing Pregnancy Rates in Frozen–Thawed Embryo Transfer Cycles: A Retrospective Analysis of 2,562 Cycles
Authors: Tian Haiqing , Zhang Hejiang , Qiu Hong , Yang Xuejiao , La Xiaolin , Cui Lei
Published: May 2022

Background

In frozen–thawed embryo transfer (FET) cycles, endometrial thickness (EMT) has been used routinely as the main clinical monitoring index. However, the current findings are conflicting.

Method

This was a single-center retrospective study of 2,054 couples (2,562 cycles) who underwent FET (including cleavage stage embryos and blastocysts) between January 2017 and August 2020 in the reproductive centers of First Affiliated Hospital of Xinjiang Medical University. The primary outcome measure was the ongoing pregnancy rate (OPR); the secondary outcome was the clinical pregnancy rate.

Results

After stratified analysis and adjusting for confounders such as maternal age, duration of infertility, number of high-quality embryos transferred, endometrial preparation protocol, number of transfer cycles, and stages of embryo transferred, we found a curvilinear relationship between EMT and the OPR in women < 35 years of age. For women with EMT ≤ 8 mm, the OPR increased by 150% for cleavage stage embryo transfer for every 1 mm increase in the EMT; similarly, it increased by 97% for blastocyst stage FET. However, there was a linear relationship between EMT and OPR in women aged ≥ 35 years. When blastocysts were transferred, for every 1 mm increase in the EMT the OPR increased significantly by 12%. But OPR after frozen-thawed cleavage stage embryos transfer did not increase significantly with increased EMT

Conclusions

Our study showed that the OPR increased significantly with increased EMT between young women aged < 35 years with EMT ≤ 8 mm and older women who underwent transfer of blastocysts.
Positive Effects of Extra-Virgin Olive Oil Supplementation and DietBra on Inflammation and Glycemic Profiles in Adults With Type 2 Diabetes and Class II/III Obesity: A Randomized Clinical Trial
Authors: Silveira Erika Aparecida , Rosa Lorena Pereira de Souza , de Resende Danilo Pires , Rodrigues Ana Paula dos Santos , da Costa Adeliane Castro , Rezende Andréa Toledo de Oliveira , Noll Matias , de Oliveira Cesar , Junqueira-Kipnis Ana Paula
Published: May 2022

Background

Evidence on the effects of dietary interventions on inflammatory markers in individuals with obesity and type 2 diabetes mellitus (T2DM) is scarce. Our study evaluated the effects of extra-virgin olive oil alone and in combination with a traditional Brazilian diet on inflammatory markers and glycemic profiles in adults with both T2DM and class II/III obesity.

Methods

Adults aged 18-64 years with T2DM and class II/III obesity were randomized into two intervention groups: 1) extra-virgin olive oil only and 2) extra-virgin olive oil + a traditional Brazilian diet (OliveOil+DietBra). Data on sociodemographic characteristics, lifestyle, anthropometry, biochemical markers and inflammatory markers were collected. The primary outcomes were glycemic parameters and inflammatory markers. The body mass index (BMI) and weight were the secondary outcomes.

Results

Forty individuals with T2DM and class II/III obesity were enrolled, and 34 (85%) completed the intervention course. The intake of olive oil was 37.88 ± 12.50 mL/day in the olive oil group and 37.71 ± 12.23 mL/day in the OliveOil+DietBra group, with no significant difference between groups (p = 0.484). Compared to the olive oil only group, the OliveOil+DietBra group had significantly lower levels of fasting insulin (p = 0.047) at the end of the intervention, whereas the other glycemic parameters were not altered. In the OliveOil+DietBra group, serum levels of inflammatory cytokines, IL-1α (p = 0.006) and adiponectin (p = 0.049) were lower and those of TNFα were higher (p = 0.037). There was a significant reduction in BMI and weight compared to the baseline values in the OliveOil+DietBra group (p = 0.015).

Conclusions

The intervention with OliveOil+DietBra effectively decreased the levels of fasting insulin, IL-1α and adiponectin, suggesting its beneficial role in improving the inflammatory profiles and fasting insulin levels in adults with class II/III obesity and T2DM.

Clinical Trial Registration

ClinicalTrials.gov, identifier: NCT02463435
COVID-19 Vaccination in Patients With Malignancy; A Systematic Review and Meta-Analysis of the Efficacy and Safety
Authors: Javadinia Seyed Alireza , Alizadeh Kimia , Mojadadi Mohammad-Shafi , Nikbakht Fateme , Dashti Farzaneh , Joudi Maryam , Harati Hadi , Welsh James S. , Farahmand Seyed Amir , Attarian Fahimeh
Published: May 2022

Background

Data on the efficacy and safety of COVID-19 vaccines in patients with malignancy are immature. In this paper, we assessed the literature involving the use of COVID-19 vaccines in cancer patients and reported the seroconversion rates as the main outcome and severity of COVID-19 infection and side effects following COVID-19 vaccination as the secondary outcomes.

Methods

A systematic review with meta-analysis was performed. Searches were conducted in electronic websites, databases, and journals, including Scopus, PubMed, Embase, and Web of Science from January 01, 2019, to November 30, 2021. Studies reporting data on the safety and efficacy of COVID vaccine in cancer patients using any human samples were included. The risk of bias was assessed using the NEWCASTLE-OTTAWA scale in the included studies.

Results

A total of 724 articles were identified from databases, out of which 201 articles were duplicates and were discarded. Subsequently, 454 articles were excluded through initial screening of the titles and abstracts. Moreover, 41 studies did not report the precise seroconversion rate either based on the type of cancer or after injection of a second dose of COVID vaccine. Finally, 28 articles met all the inclusion criteria and were included in this systematic review. The overall seroconversion rates after receiving a second dose of COVID-19 vaccine, based on type of cancer were 88% (95% CI, 81%-92%) and 70% (95% CI, 60%-79%) in patients with solid tumors and hematologic malignancies, respectively.

Conclusion

Overall, we conclude that vaccination against COVID-19 in patients with active malignancies using activated and inactivated vaccines is a safe and tolerable procedure that is also accompanied by a high efficacy.
Climate Change Impact on the Offshore Wind Energy Over the North Sea and the Irish Sea
Authors: Susini Stefano , Menendez Melisa , Eguia Pablo , Blanco Jesus Maria
Published: May 2022

The impact of climate change on the environment and human activities is one of the biggest concerns for the international community. Wind energy represents one of the most reliable and promising technology to achieve the target reduction of emissions. Though more intense and uniform resources characterize offshore areas, climate change may alter the environmental conditions and thus Levelized Cost of Energy evaluation. In this study, we analyze the impact of climate change on the offshore wind energy sector over the North Sea and the Irish Sea, where the majority of the European investments are located. To this aim, seven regional climate model simulations from the EURO-Cordex project are first evaluated. The ERA5 reanalysis product is considered the historical reference information after its validation against in-situ records and it is used to analyze the climate simulations by assessing their performance to reproduce weather types. Several statistics are calculated to assess the skill of each model in reproducing past climatology for the reference period (1985-2004). Since no significant differences between simulations are highlighted, an ensemble of all the seven simulations is used to characterize future changes in the offshore climate. Weather types under the representative concentration path scenario RCP8.5 for the future period 2081-2100 are then analyzed to describe the changes in climatological mean and extreme events. Regional climate model simulations are bias-corrected by applying the empirical quantile mapping technique. Then, future changes in six wind energy climate indicators (i.e. mean and extreme wind speed, wind power density, operation hours, gross energy yield, and capacity factor) are estimated for seven operating offshore wind farms. Results indicate a slight decrease in wind energy production, particularly in the northwest of the domain of study, testified by a reduction of all the climate indicators. However, large uncertainties in the projected changes are found at the wind farms located close to the south coast of the North Sea. Extreme wind conditions show a modest rise in the southeastern part of the region, related to an increase of the weather types dominated by cyclonic systems off Scotland shores.
Annual CO2 Budget Estimation From Chamber-Based Flux Measurements on Intensively Drained Peat Meadows: Effect of Gap-Filling Strategies
Authors: Liu Weier , Fritz Christian , Weideveld Stefan T. J. , Aben Ralf C. H. , van den Berg Merit , Velthuis Mandy
Published: May 2022

Estimating annual CO2 budgets on drained peatlands is important in understanding the significance of CO2 emissions from peatland degradation and evaluating the effectiveness of mitigation techniques. The closed-chamber technique is widely used in combination with gap-filling of CO2 fluxes by parameter fitting empirical models of ecosystem respiration (Reco) and gross primary production (GPP). However, numerous gap-filling strategies are available which are suitable for different circumstances and can result in large variances in annual budget estimates. Therefore, a need for guidance on the selection of gap-filling methodology and its influence on the results exists. Here, we propose a framework of gap-filling methods with four Tiers following increasing model complexity at structural and temporal levels. Tier one is a simple parameter fitting of basic empirical models on an annual basis. Tier two adds structural complexity by including extra environmental factors such as grass height, groundwater level and drought condition. Tier three introduces temporal complexity by separation of annual datasets into seasons. Tier four is a campaign-specific parameter fitting approach, representing highest temporal complexity. The methods were demonstrated on two chamber-based CO2 flux datasets, one of which was previously published. Performance of the empirical models were compared in terms of error statistics. Annual budget estimates were indirectly validated with carbon export values. In conclusion, different gap-filling methodologies gave similar annual estimates but different intra-annual CO2 fluxes, which did not affect the detection of the treatment effects. The campaign-wise gap-filling at Tier four gave the best model performances, while Tier three seasonal gap-filling produced satisfactory results throughout, even under data scarcity. Given the need for more complete carbon balances in drained peatlands, our four-Tier framework can serve as a methodological guidance to the handling of chamber-measured CO2 fluxes, which is fundamental in understanding emissions from degraded peatlands and its mitigation. The performance of models on intra-annual data should be validated in future research with continuous measured CO2 flux data.
Bacterial Communities and Culturable Petroleum Hydrocarbon Degrading Bacteria in Marine Sediments in the Northeastern South China Sea
Authors: Xu Wenhui , Qiao Yanlu , Wei Jinxiao , Jiang Qing , Xue Jianliang
Published: May 2022

Marine oil spill pollution was one of the factors affecting the marine ecology of the northeastern South China Sea (nSCS). The submarine oil produced after the oil spill had a long-term impact on the microbial community in the sediment. In this study, 16S rRNA genes high-throughput sequencing and quantitative PCR were used to study the composition and distribution of bacterial communities in deep-sea sediments; meanwhile, petroleum hydrocarbon degrading bacteria were isolated, of which activity were detected. Proteobacteria and Planctomycetota were the main bacterial phyla found in the samples studied in this study. 29 bacterial strains capable of degrading petroleum hydrocarbons were isolated from S02 and S39 sediment samples, belonging to genus Stenotrophomonas, Pseudidiomarina, Sulfitobacter, Pseudomonas, Halomonas and so on. Strains from Stenotrophomonas degraded petroleum hydrocarbons efficiently. This research provided new insights into distribution pattern of benthic microbial community in the nSCS, and validated the degradation potential of petroleum hydrocarbons by indigenous bacteria.
Biocultural Drivers Responsible for the Occurrence of a Cassava Bacterial Pathogen in Small-Scale Farms of Colombian Caribbean
Authors: Pérez Darío , Duputié Anne , Vernière Christian , Szurek Boris , Caillon Sophie
Published: May 2022

Cassava (Manihot esculenta Crantz) is a primary crop for food security of millions of people worldwide. In Colombia, the Caribbean region contributes about half of the national cassava production, despite major socioeconomic constraints such as unequal land property, omnipresence of middlemen, low and unstable prices, armed conflict, climate change and phytosanitary issues. Among the latter is Cassava Bacterial Blight (CBB), a disease caused by the bacterial pathogen Xanthomonas phaseoli pv. manihotis (Xpm) that leads to irreversible damage to plants, impeding growth and productivity. In 2016, we analyzed the role of sociocultural and agricultural practices on CBB prevalence in small-scale fields of a village of the Colombian Caribbean region, where farmers live almost exclusively from the sale of their cassava production. Semi-structured interviews (48) were conducted with all farmers who cultivated cassava to document individual sociodemographic characteristics, cassava farming practices, and perceptions about CBB occurrence. Cassava Bacterial Blight was diagnosed in the field and the presence of Xpm was further confirmed upon laboratory analysis of collected diseased leaf samples. Our data show that (i) according to the risks perceived by farmers, CBB is the main disease affecting cassava crops in the village and it could indeed be detected in about half of the fields visited; (ii) CBB occurrence depends strongly on land property issues, likely because of an inadequate phytosanitary control during acquisition of cuttings when farmers are forced to rent the land; and (iii) there is a strong positive correlation between the use of commercial fertilizers and the occurrence of CBB in the village of Villa López.
Microeukaryotic Community Shifting Along a Lentic-Lotic Continuum
Authors: Wang Yixia , Ren Ze , He Ping , Xu Jie , Li Daikui , Liu Cunqi , Liu Bo , Wu Naicheng
Published: May 2022

As an important regulator of ecosystem functions in river systems, microeukaryotes play an important role in energy and material conversion, yet little is known about the shift along a lentic-lotic continuum. In this study, the 18S rRNA genes sequencing was used to identify the microeukaryotic communities at 82 sites along a lentic-lotic continuum with the aim of understanding the impact of upstream inlet river on microeukaryotic communities in Baiyang Lake (BYD) and its downstream. Our results showed that the upstream inlet river affected the diversity and community composition of microeukaryotes in BYD and downstream rivers, and environmental variables greatly affected the composition of microeukaryotic community. The community composition in BYD had lower variabilities. Co-occurrence network analysis revealed that the network was non-random and clearly parsed into three modules, and different modules were relatively more abundant to a particular area. As keystone taxa, some nodes of the upstream microeukaryotic network played an important role in structuring network and maintaining the stability of the ecosystem. In BYD and downstream, the microeukaryotic network was highly fragmented, and the loss of keystone taxa would have an adverse impact on the integrity and function of the microeukaryotic community. Microeukaryotes had strong tendencies to co-occur, which may contribute to the stability and resilience of microeukaryotic communities. Overall, these findings extend the current understanding of the diversity and community composition of microeukaryotic along a lentic-lotic continuum.
Magnesium- a Forgotten Element: Phenotypic Variation and Genome Wide Association Study in Turkish Common Bean Germplasm
Authors: Baloch Faheem Shehzad , Nadeem Muhammad Azhar , Sönmez Ferit , Habyarimana Ephrem , Mustafa Zemran , Karaköy Tolga , Cömertpay Gönül , Alsaleh Ahmad , Çiftçi Vahdettin , Sun Sangmi , Chung Gyuhwa , Chung Yong Suk
Published: May 2022

Magnesium (Mg) is the fourth most abundant element in the human body and plays the role of cofactor for more than 300 enzymatic reactions. In plants, Mg is involved in various key physiological and biochemical processes like growth, development, photophosphorylation, chlorophyll formation, protein synthesis, and resistance to biotic and abiotic stresses. Keeping in view the importance of this element, the present investigation aimed to explore the Mg contents diversity in the seeds of Turkish common bean germplasm and to identify the genomic regions associated with this element. A total of 183 common bean accessions collected from 19 provinces of Turkey were used as plant material. Field experiments were conducted according to an augmented block design during 2018 in two provinces of Turkey, and six commercial cultivars were used as a control group. Analysis of variance depicted that Mg concentration among common bean accessions was statistically significant (p < 0.05) within each environment, however genotype × environment interaction was non-significant. A moderate level (0.60) of heritability was found in this study. Overall mean Mg contents for both environments varied from 0.33 for Nigde-Dermasyon to 1.52 mg kg−1 for Nigde-Derinkuyu landraces, while gross mean Mg contents were 0.92 mg kg−1. At the province level, landraces from Bolu were rich while the landraces from Bitlis were poor in seed Mg contents respectively. The cluster constellation plot divided the studied germplasm into two populations on the basis of their Mg contents. Marker-trait association was performed using a mixed linear model (Q + K) with a total of 7,900 DArTseq markers. A total of six markers present on various chromosomes (two at Pv01, and one marker at each chromosome i.e., Pv03, Pv07, Pv08, Pv11) showed statistically significant association for seed Mg contents. Among these identified markers, the DArT-3367607 marker present on chromosome Pv03 contributed to maximum phenotypic variation (7.5%). Additionally, this marker was found within a narrow region of previously reported markers. We are confident that the results of this study will contribute significantly to start common bean breeding activities using marker assisted selection regarding improved Mg contents.
Regulation of Plant Tannin Synthesis in Crop Species
Authors: Mora José , Pott Delphine M. , Osorio Sonia , Vallarino José G.
Published: May 2022

Plant tannins belong to the antioxidant compound family, which includes chemicals responsible for protecting biological structures from the harmful effects of oxidative stress. A wide range of plants and crops are rich in antioxidant compounds, offering resistance to biotic, mainly against pathogens and herbivores, and abiotic stresses, such as light and wound stresses. These compounds are also related to human health benefits, offering protective effects against cardiovascular and neurodegenerative diseases in addition to providing anti-tumor, anti-inflammatory, and anti-bacterial characteristics. Most of these compounds are structurally and biosynthetically related, being synthesized through the shikimate-phenylpropanoid pathways, offering several classes of plant antioxidants: flavonoids, anthocyanins, and tannins. Tannins are divided into two major classes: condensed tannins or proanthocyanidins and hydrolysable tannins. Hydrolysable tannin synthesis branches directly from the shikimate pathway, while condensed tannins are derived from the flavonoid pathway, one of the branches of the phenylpropanoid pathway. Both types of tannins have been proposed as important molecules for taste perception of many fruits and beverages, especially wine, besides their well-known roles in plant defense and human health. Regulation at the gene level, biosynthesis and degradation have been extensively studied in condensed tannins in crops like grapevine (Vitis vinifera), persimmon (Diospyros kaki) and several berry species due to their high tannin content and their importance in the food and beverage industry. On the other hand, much less information is available regarding hydrolysable tannins, although some key aspects of their biosynthesis and regulation have been recently discovered. Here, we review recent findings about tannin metabolism, information that could be of high importance for crop breeding programs to obtain varieties with enhanced nutritional characteristics.
ILF2 Contributes to Hyperproliferation of Keratinocytes and Skin Inflammation in a KLHDC7B-DT-Dependent Manner in Psoriasis
Authors: Yin Xiran , Yang Zhenxian , Zhu Mingsheng , Chen Cheng , Huang Shan , Li Xueqing , Zhong Hua , Wen He , Sun Qing , Yu Xiaojing , Yan Jianjun
Published: May 2022

Background: The extensive involvement of interleukin enhancer binding factor 2 (ILF2) in RNA stability and the inflammatory response is well documented. Aberrant long noncoding RNA (lncRNA) expression contributes to the pathogenesis of psoriasis. However, little is known about the role of ILF2 in psoriasis.

Objective: To investigate the role of ILF2 and KLHDC7B-DT in psoriasis.

Methods: LncRNA expression in psoriatic tissues was measured by lncRNA microarray and qRT–PCR. Normal human epidermal keratinocytes (NHEKs), HaCaT cells, and Ker-CT cells stimulated with M5 (IL-17A, IL-22, IL-1α, oncostatin M, and TNF-α) were used to establish a psoriasis model in vitro. Fluorescence in situ hybridization was used to detect the distribution of KLHDC7B-DT and ILF2 in keratinocytes. The proliferative effects of KLHDC7B-DT and ILF2 on keratinocytes were demonstrated by EdU assay and flow cytometry. ELISA was used to detect the secretion levels of cytokines. RNA pull-down and RNA immunoprecipitation (RIP) were used to detect the direct binding of KLHDC7B-DT with ILF2. Western blotting was used to detect the proteins related to STAT3/JNK signalling pathways.

Results: ILF2 and KLHDC7B-DT were significantly overexpressed in psoriatic tissues and M5-induced keratinocytes. KLHDC7B-DT promoted the proliferation of keratinocytes and induced the secretion of IL-6 and IL-8. KLHDC7B-DT could directly bind to ILF2 and activate the STAT3 and JNK signalling pathways. KLHDC7B-DT expression was regulated by ILF2. M5-induced proliferation and inflammatory cytokine secretion in keratinocytes was inhibited after ILF2 knockdown. Furthermore, we found that ILF2 promoted keratinocyte proliferation and the inflammatory response in a KLHDC7B-DT-dependent manner.

Conclusions: ILF2 and KLHDC7B-DT are involved in the hyperproliferation of keratinocytes and skin inflammation in psoriasis. In addition, ILF2 functions in a KLHDC7B-DT-dependent manner.
Identifying Potential Clusters of Future Migration Associated With Water Stress in Africa: A Vulnerability Approach
Authors: de Bruin Sophie Pieternel , Knoop Joost , Visser Hans , Biemans Hester
Published: May 2022

Decreasing yields due to water stress form a threat to rural livelihoods and can affect migration dynamics, especially in vulnerable regions that lack the capacity to adapt agriculture to water stress. But since migration is complex, non-linear and context-dependent, it is not feasible to predict the precise number of people that will migrate due to water stress. It is possible to map the different conditions that shape regional vulnerabilities and the number of people affected. This study presents a vulnerability approach to identify areas on the African continent where emigration associated with water stress is expected to be relatively high by 2050 under a middle-of-the-road scenario (SSP2) and compares the results with the 2010 situation. By utilizing among other indicators the water yield gap, the impact of water stress on rainfed agricultural crop yields is included, reflecting the impact of water stress on rural livelihoods depending on crop farming. The analysis was done on a water-province level, 393 in total. Clusters of potential emigration associated with the impacts of water stress on agriculture are projected for parts of the Sahel, the Horn of Africa and regions of Angola. The regions where migration associated with water stress is expected to be relatively high by 2050 are approximately the same as those of 2010, although more people are projected to be living in these water-stressed regions. By developing this vulnerability approach, this manuscript enlarges the current insights regarding future clusters of water stress-related migration.
Small Molecule Agents Targeting PD-1 Checkpoint Pathway for Cancer Immunotherapy: Mechanisms of Action and Other Considerations for Their Advanced Development
Authors: Sasikumar Pottayil G. , Ramachandra Murali
Published: May 2022

Pioneering success of antibodies targeting immune checkpoints such as programmed cell death protein 1 (PD-1) and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) has changed the outlook of cancer therapy. Although these antibodies show impressive durable clinical activity, low response rates and immune-related adverse events are becoming increasingly evident in antibody-based approaches. For further strides in cancer immunotherapy, novel treatment strategies including combination therapies and alternate therapeutic modalities are highly warranted. Towards this discovery and development of small molecule, checkpoint inhibitors are actively being pursued, and the efforts have culminated in the ongoing clinical testing of orally bioavailable checkpoint inhibitors. This review focuses on the small molecule agents targeting PD-1 checkpoint pathway for cancer immunotherapy and highlights various chemotypes/scaffolds and their characterization including binding and functionality along with reported mechanism of action. The learnings from the ongoing small molecule clinical trials and crucial points to be considered for their clinical development are also discussed.
Arctic and Atlantic Waters in the Norwegian Basin, Between Year Variability and Potential Ecosystem Implications
Authors: Skagseth Øystein , Broms Cecilie , Gundersen Kjell , Hátún Hjálmar , Kristiansen Inga , Larsen Karin Margretha H. , Mork Kjell Arne , Petursdottir Hildur , Søiland Henrik
Published: May 2022

The ocean climate of the southern Norwegian Sea - the Norwegian Basin - is largely set by the relative amount of Atlantic Water in the eastern and Arctic Water in the western region. Here we utilized hydrographic data from repeated sections, together with annually gridded survey data of the upper 1000 m, to resolve the main hydrographic changes over the period 1995-2019. Based on integrated heat -and freshwater content, we divide into three periods. The first period 1995-2005, denoted Arctic, is characterized by relative fresh and cold Atlantic Water overlaying Arctic Intermediate Water that basically covers the whole Norwegian Basin. Differently, the conditions during the period 2006-2016, denoted Atlantic, are warmer and more saline, and the extent and thickness of Arctic Intermediate Water is greatly reduced. During the most recent period denoted Fresh, 2017-2019, there has been a major freshening of the Atlantic waters, the layer of Arctic Intermediate Water has not recovered, but instead a layer of warmer but relative fresh Arctic Water has expanded. We find that increased abundance of the Arctic zooplankton Calanus hyperboreus in the southern and eastern Norwegian Basin coincides with increased extent of Arctic Water. We also note that the overall mesozooplankton biomass in the Norwegian Basin is significantly higher during periods of relative high amount of Arctic Water. Furthermore, we show that both nitrate and silicate winter (pre-bloom) concentrations are significantly higher in the Arctic Water compared to Atlantic Water, and that there is a reduction in nutrients from the Arctic period compared subsequent Atlantic and Fresh periods. Since these nutrients can be interpreted as the potential for new production, changes in the influx of western Arctic waters are expected to have a bottom-up effect on the Norwegian Sea. Hence, this study indicates that the amount of Arctic waters and their concentration of nutrients and zooplankton are more important for the Norwegian Basin ecosystem functioning rather than the temperature of the Atlantic waters.
A Code of Conduct Is Imperative for Ocean Carbon Dioxide Removal Research
Authors: Loomis Rebecca , Cooley Sarah R. , Collins James R. , Engler Simon , Suatoni Lisa
Published: May 2022

As the impacts of rising temperatures mount and the global transition to clean energy advances only gradually, scientists and policymakers are looking towards carbon dioxide removal (CDR) methods to prevent the worst impacts of climate change. Attention has increasingly focused on ocean CDR techniques, which enhance or restore marine systems to sequester carbon. Ocean CDR research presents the risk of uncertain impacts to human and environmental welfare, yet there are no domestic regulations aimed at ensuring the safety and efficacy of this research. A code of conduct that establishes principles of responsible research, fairness, and equity is needed in this field. This article presents fifteen key components of an ocean CDR research code of conduct.
Proteomic Approaches to Unravel Mechanisms of Antibiotic Resistance and Immune Evasion of Bacterial Pathogens
Authors: Torres-Sangiao Eva , Giddey Alexander Dyason , Leal Rodriguez Cristina , Tang Zhiheng , Liu Xiaoyun , Soares Nelson C.
Published: May 2022

The profound effects of and distress caused by the global COVID-19 pandemic highlighted what has been known in the health sciences a long time ago: that bacteria, fungi, viruses, and parasites continue to present a major threat to human health. Infectious diseases remain the leading cause of death worldwide, with antibiotic resistance increasing exponentially due to a lack of new treatments. In addition to this, many pathogens share the common trait of having the ability to modulate, and escape from, the host immune response. The challenge in medical microbiology is to develop and apply new experimental approaches that allow for the identification of both the microbe and its drug susceptibility profile in a time-sensitive manner, as well as to elucidate their molecular mechanisms of survival and immunomodulation. Over the last three decades, proteomics has contributed to a better understanding of the underlying molecular mechanisms responsible for microbial drug resistance and pathogenicity. Proteomics has gained new momentum as a result of recent advances in mass spectrometry. Indeed, mass spectrometry-based biomedical research has been made possible thanks to technological advances in instrumentation capability and the continuous improvement of sample processing and workflows. For example, high-throughput applications such as SWATH or Trapped ion mobility enable the identification of thousands of proteins in a matter of minutes. This type of rapid, in-depth analysis, combined with other advanced, supportive applications such as data processing and artificial intelligence, presents a unique opportunity to translate knowledge-based findings into measurable impacts like new antimicrobial biomarkers and drug targets. In relation to the Research Topic “Proteomic Approaches to Unravel Mechanisms of Resistance and Immune Evasion of Bacterial Pathogens,” this review specifically seeks to highlight the synergies between the powerful fields of modern proteomics and microbiology, as well as bridging translational opportunities from biomedical research to clinical practice.
Comparison of Indocyanine Green Angiography and Swept-Source Wide-Field Optical Coherence Tomography Angiography in Posterior Uveitis
Authors: Tian Meng , Zeng Guodong , Tappeiner Christoph , Zinkernagel Martin S. , Wolf Sebastian , Munk Marion R.
Published: May 2022

Purpose

To compare indocyanine green angiography (ICGA) and swept-source wide-field optical coherence tomography angiography (SS-OCTA) for the assessment of patients with posterior uveitis.

Method

SS-OCTA montage images of 5 x 12 x 12 mm or 2 x 15 x 9 mm, covering ~70–90 degree of the retina of consecutive patients with posterior uveitis were acquired. The choriocapillaries and choroidal slabs were compared to findings on ICGA.

Results

Sixty-eight eyes of 41 patients were included (mean age 47.2 ± 20.4 years; 58.5% female). In 23 (34%) lesions were visible on OCTA, but not discernable on ICGA. In turn, out of the 45 eyes with clearly discernable lesions on ICGA, 22 (49%) and 21 (47%) eyes showed no corresponding areas of flow deficit on OCTA in the CC and choroidal slab, respectively. Lesion size strongly correlated among ICGA and OCTA choriocapillaries- (CC) (r = 0.99, p ≤ 0.0001) and choroidal slabs (r = 0.99, p ≤ 0.0001), respectively. The mean lesion size on the late frames of ICGA (8.45 ± 5.47 mm2) was larger compared to the lesion size on OCTA CC scan (7.98 ± 5.47 mm2, p ≤ 0.0001) and choroidal scan (7.69 ± 5.10 mm2, p = 0.002), respectively. The lesion size on OCTA CC scan was significantly larger than on the OCTA choroidal scan (p ≤ 0.0001).

Conclusion

SS-wide field OCTA may be a promising tool to assess posterior uveitis patients and may replace ICGA to a certain extent in the future.
Surviving in the Brine: A Multi-Omics Approach for Understanding the Physiology of the Halophile Fungus Aspergillus sydowii at Saturated NaCl Concentration
Authors: Jiménez-Gómez Irina , Valdés-Muñoz Gisell , Moreno-Ulloa Aldo , Pérez-Llano Yordanis , Moreno-Perlín Tonatiuh , Silva-Jiménez Hortencia , Barreto-Curiel Fernando , Sánchez-Carbente María del Rayo , Folch-Mallol Jorge Luis , Gunde-Cimerman Nina , Lago-Lestón Asunción , Batista-García Ramón Alberto
Published: May 2022

Although various studies have investigated osmoadaptations of halophilic fungi to saline conditions, only few analyzed the fungal mechanisms occurring at saturated NaCl concentrations. Halophilic Aspergillus sydowii is a model organism for the study of molecular adaptations of filamentous fungi to hyperosmolarity. For the first time a multi-omics approach (i.e., transcriptomics and metabolomics) was used to compare A. sydowii at saturated concentration (5.13 M NaCl) to optimal salinity (1 M NaCl). Analysis revealed 1,842 genes differentially expressed of which 704 were overexpressed. Most differentially expressed genes were involved in metabolism and signal transduction. A gene ontology multi-scale network showed that ATP binding constituted the main network node with direct interactions to phosphorelay signal transduction, polysaccharide metabolism, and transferase activity. Free amino acids significantly decreased and amino acid metabolism was reprogrammed at 5.13 M NaCl. mRNA transcriptional analysis revealed upregulation of genes involved in methionine and cysteine biosynthesis at extreme water deprivation by NaCl. No modifications of membrane fatty acid composition occurred. Upregulated genes were involved in high-osmolarity glycerol signal transduction pathways, biosynthesis of β-1,3-glucans, and cross-membrane ion transporters. Downregulated genes were related to the synthesis of chitin, mannose, cell wall proteins, starvation, pheromone synthesis, and cell cycle. Non-coding RNAs represented the 20% of the total transcripts with 7% classified as long non-coding RNAs (lncRNAs). The 42% and 69% of the total lncRNAs and RNAs encoding transcription factors, respectively, were differentially expressed. A network analysis showed that differentially expressed lncRNAs and RNAs coding transcriptional factors were mainly related to the regulation of metabolic processes, protein phosphorylation, protein kinase activity, and plasma membrane composition. Metabolomic analyses revealed more complex and unknown metabolites at saturated NaCl concentration than at optimal salinity. This study is the first attempt to unravel the molecular ecology of an ascomycetous fungus at extreme water deprivation by NaCl (5.13 M). This work also represents a pioneer study to investigate the importance of lncRNAs and transcriptional factors in the transcriptomic response to high NaCl stress in halophilic fungi.
Upregulation of tRNA-Ser-AGA-2-1 Promotes Malignant Behavior in Normal Bronchial Cells
Authors: Santos Mafalda , Fidalgo Ana , Varanda Ana Sofia , Soares Ana Raquel , Almeida Gabriela M. , Martins Diana , Mendes Nuno , Oliveira Carla , Santos Manuel A. S.
Published: May 2022

Serine tRNAs (tRNASer) are frequently overexpressed in tumors and associated with poor prognosis and increased risk of recurrence in breast cancer. Impairment of tRNA biogenesis and abundance also impacts proteome homeostasis, and activates protein quality control systems. Herein, we aimed at testing whether increasing tRNASer abundance could foster tumor establishment through activation of the UPR. In order to do so, firstly we confirmed that the expression of tRNA-Ser-AGA-2-1 [hereafter tRNASer(AGA)] was upregulated by 1.79-fold in Stage I NSCLC tumors when compared to normal adjacent tissue. To study the impact of tRNASer(AGA) in early stage tumorigenesis, we induced its upregulation in a non-tumoral bronchial cell line, BEAS-2B. Upregulation of this tRNA increased cellular proliferation and protein synthesis rate, driven by eIF2α dephosphorylation and ATF4 activation downstream of PERK signaling. Futhermore, tRNASer(AGA) enhanced transformation potential in vitro, and promoted the establishment of slow growing tumors with aggressive features in nude mice. Our work highlights the importance of studying tRNA deregulation on early stage tumorigenesis, as they may be potential malignancy and aggressiveness biomarkers.
Development of a Polymerase Spiral Reaction-Based Isothermal Assay for Rapid Identification of Thrips palmi
Authors: Jangra Sumit , Ghosh Amalendu , Mukherjee Sunil , Baranwal Virendra Kumar , Dietzgen Ralf G.
Published: May 2022

Thrips cause considerable economic losses to a wide range of food, feed, and forest crops. They also transmit several plant viruses. Being cryptic, it is often difficult to distinguish thrips species in crops and large consignments by conventional methods. Melon thrips (Thrips palmi Karny, Thysanoptera: Thripidae) is an invasive insect pest of vegetables, legumes, and ornamentals besides being vector to several viruses. It poses a threat to domestic and international plant biosecurity and can invade and establish in new areas. Here, we report a polymerase spiral reaction (PSR)-based isothermal assay for rapid, sensitive, specific, low-cost, and on-site detection of T. palmi. To the best of our knowledge, this is the first application of PSR in the identification of any insect species. A primer pair designed based on 3′-polymorphism of mtCOIII region can specifically identify T. palmi without any cross-reactivity with predominant thrips species. The assay uses crude lysate of a single thrips saving time and reagents involved in nucleic acid extraction. The presence of T. palmi is visualized by the appearance of bright fluorescence under ultraviolet light or a change in reaction color thus avoiding gel electrophoresis steps. The entire process can be completed in 70 min on-site using only an ordinary water bath. The assay is sensitive to detecting as little as 50 attograms of T. palmi template. The assay was validated with known thrips specimens and found to be efficient in diagnosing T. palmi under natural conditions. The described method will be useful for non-expert personnel to detect an early infestation, accidental introduction to a new area, restrict the spread of diseases and formulate appropriate management strategies.
Inverse Problem Reveals Conditions for Characteristic Retinal Degeneration Patterns in Retinitis Pigmentosa Under the Trophic Factor Hypothesis
Authors: Roberts Paul A.
Published: May 2022

Retinitis pigmentosa (RP) is the most common inherited retinal dystrophy with a prevalence of about 1 in 4,000, affecting approximately 1.5 million people worldwide. Patients with RP experience progressive visual field loss as the retina degenerates, destroying light-sensitive photoreceptor cells (rods and cones), with rods affected earlier and more severely than cones. Spatio-temporal patterns of retinal degeneration in human RP have been well characterised; however, the mechanism(s) giving rise to these patterns have not been conclusively determined. One such mechanism, which has received a wealth of experimental support, is described by the trophic factor hypothesis. This hypothesis suggests that rods produce a trophic factor necessary for cone survival; the loss of rods depletes this factor, leading to cone degeneration. In this article, we formulate a partial differential equation mathematical model of RP in one spatial dimension, spanning the region between the retinal centre (fovea) and the retinal edge (ora serrata). Using this model we derive and solve an inverse problem, revealing for the first time experimentally testable conditions under which the trophic factor mechanism will qualitatively recapitulate the spatio-temporal patterns of retinal regeneration observed in human RP.