bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Protein Biomarkers for Differentiating Listeria monocytogenes Genetic Lineage III
Authors: Gomaa Basant , Lu Jingjun , Abdelhamed Hossam , Banes Michelle , Pechanova Olga , Pechan Tibor , Arick Mark A. , Karsi Attila , Lawrence Mark L.
Published: April 2024

Listeria monocytogenes is the causative agent of listeriosis, a severe foodborne illness characterized by septicemia, meningitis, encephalitis, abortions, and occasional death in infants and immunocompromised individuals. L. monocytogenes is composed of four genetic lineages (I, II, III, and IV) and fourteen serotypes. The aim of the current study was to identify proteins that can serve as biomarkers for detection of genetic lineage III strains based on simple antibody-based methods. Liquid chromatography (LC) with electrospray ionization tandem mass spectrometry (ESI MS/MS) followed by bioinformatics and computational analysis were performed on three L. monocytogenes strains (NRRL B-33007, NRRL B-33014, and NRRL B-33077), which were used as reference strains for lineages I, II, and III, respectively. Results from ESI MS/MS revealed 42 unique proteins present in NRRL B-33077 and absent in NRRL B-33007 and NRRL B-33014 strains. BLAST analysis of the 42 proteins against a broader panel of >80 sequenced strains from lineages I and II revealed four proteins [TM2 domain-containing protein (NRRL B-33077_2770), DUF3916 domain-containing protein (NRRL B-33077_1897), DNA adenine methylase (NRRL B-33077_1926), and protein RhsA (NRRL B-33077_1129)] that have no homology with any sequenced strains in lineages I and II. The four genes that encode these proteins were expressed in Escherichia coli strain DE3 and purified. Polyclonal antibodies were prepared against purified recombinant proteins. ELISA using the polyclonal antibodies against 12 L. monocytogenes lineage I, II, and III isolates indicated that TM2 protein and DNA adenine methylase (Dam) detected all lineage III strains with no reaction to lineage I and II strains. In conclusion, two proteins including TM2 protein and Dam are potentially useful biomarkers for detection and differentiation of L. monocytogenes lineage III strains in clinical, environmental, and food processing facilities. Furthermore, these results validate the approach of using a combination of proteomics and bioinformatics to identify useful protein biomarkers.
High-Pressure Processing Effects on Microbiological Stability, Physicochemical Properties, and Volatile Profile of a Fruit Salad
Authors: Lopes Ana C. , Queirós Rui P. , Inácio Rita S. , Pinto Carlos A. , Casal Susana , Delgadillo Ivonne , Saraiva Jorge A.
Published: April 2024

Nowadays, consumers are more aware of the effects of their diet on their health, and thus demand natural or minimally processed food products. Therefore, research has focused on processes that assure safe products without jeopardizing their nutritional properties. In this context, this work aimed to evaluate the effects of high-pressure processing (550 MPa/3 min/15 °C, HPP) on a fruit salad (composed of melon juice and pieces of Golden apple and Rocha pear) throughout 35 days of storage at 4 °C. For the physicochemical properties analysed (browning degree, polyphenol oxidase activity, antioxidant activity (ABTS assay), and volatile profile), a freshly made fruit salad was used, while for the microbiological tests (total aerobic mesophiles, and yeast and moulds) spoiled melon juice was added to the fruit salad to increase the microbial load and mimic a challenge test with a high initial microbial load. It was determined that processed samples were more microbiologically stable than raw samples, as HPP enabled a reduction of almost 4-log units of both total aerobic mesophiles and yeasts and moulds, as well as an almost 1.5-fold increase in titratable acidity of the unprocessed samples compared to HPP samples. Regarding browning degree, a significant increase (p < 0.05) was observed in processed versus unprocessed samples (roughly/maximum 68%), while the addition of ascorbic acid decreased the browning of the samples by 29%. For antioxidant activity, there were no significant differences between raw and processed samples during the 35 days of storage. An increase in the activity of polyphenol oxidase immediately after processing (about 150%) was confirmed, which was generally similar or higher during storage compared with the raw samples. Regarding the volatile profile of the product, it was seen that the compounds associated with melon represented the biggest relative percentage and processed samples revealed a decrease in the relative quantity of these compounds compared to unprocessed. Broadly speaking, HPP was shown to be efficient in maintaining the stability and overall quality of the product while assuring microbial safety (by inactivating purposely inoculated microorganisms), which allows for longer shelf life (7 versus 28 days for unprocessed and processed fruit salad, respectively).
Contemporary Views of the Extraction, Health Benefits, and Industrial Integration of Rice Bran Oil: A Prominent Ingredient for Holistic Human Health
Authors: Tufail Tabussam , Ain Huma Bader Ul , Chen Jin , Virk Muhammad Safiullah , Ahmed Zahoor , Ashraf Jawad , Shahid Noor Ul Ain , Xu Bin
Published: April 2024

Globally, 50% of people consume rice (Oryza sativa), which is among the most abundant and extensively ingested cereal grains. Rice bran is a by-product of the cereal industry and is also considered a beneficial waste product of the rice processing industry. Rice bran oil (RBO) is created from rice bran (20–25 wt% in rice bran), which is the outermost layer of the rice kernel; has a lipid content of up to 25%; and is a considerable source of a plethora of bioactive components. The main components of RBO include high levels of fiber and phytochemicals, including vitamins, oryzanols, fatty acids, and phenolic compounds, which are beneficial to human health and well-being. This article summarizes the stabilization and extraction processes of rice bran oil from rice bran using different techniques (including solvent extraction, microwaving, ohmic heating, supercritical fluid extraction, and ultrasonication). Some studies have elaborated the various biological activities linked with RBO, such as antioxidant, anti-platelet, analgesic, anti-inflammatory, anti-thrombotic, anti-mutagenic, aphrodisiac, anti-depressant, anti-emetic, fibrinolytic, and cytotoxic activities. Due to the broad spectrum of biological activities and economic benefits of RBO, the current review article focuses on the extraction process of RBO, its bioactive components, and the potential health benefits of RBO. Furthermore, the limitations of existing studies are highlighted, and suggestions are provided for future applications of RBO as a functional food ingredient.
The Generation of Suspended Cell Wall Material May Limit the Effect of Ultrasound Technology in Some Varietal Wines
Authors: Pérez-Porras Paula , Bautista-Ortín Ana Belén , Martínez-Lapuente Leticia , Guadalupe Zenaida , Ayestarán Belén , Gómez-Plaza Encarna
Published: April 2024

The disruptive effect exerted by high-power ultrasound on grape cell walls enhances phenolic extraction, improving chromatic characteristics during red wine maceration. However, short maceration times may, sometimes, hinder this enhancement, and this effect could be attributed to the suspended cell wall material formation facilitated by sonication. This suspended material, having a strong affinity for phenolic compounds, can lead to their precipitation and elimination during subsequent vinification stages and, consequently, a significant portion of extracted phenolic compounds may not contribute to the final phenolic composition of the wine, impacting its chromatic features. To demonstrate this effect, sonicated grapes of two different varieties were vinified with No modified process that eliminated part of this suspended material. Results confirm our hypothesis; that is, the lack of positive outcomes in some cases is due to phenolic compound adsorption on suspended material.
Metabolomics on Apple (Malus domestica) Cuticle—Search for Authenticity Markers
Authors: Bechynska Kamila , Sedlak Jiri , Uttl Leos , Kosek Vit , Vackova Petra , Kocourek Vladimir , Hajslova Jana
Published: April 2024

The profile of secondary metabolites present in the apple cuticular layer is not only characteristic of a particular apple cultivar; it also dynamically reflects various external factors in the growing environment. In this study, the possibility of authenticating apple samples by analyzing their cuticular layer extracts was investigated. Ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) was employed for obtaining metabolomic fingerprints. A total of 274 authentic apple samples from four cultivars harvested in the Czech Republic and Poland between 2020 and 2022 were analyzed. The complex data generated, processed using univariate and multivariate statistical methods, enabled the building of classification models to distinguish apple cultivars as well as their geographical origin. The models showed very good performance in discriminating Czech and Polish samples for three out of four cultivars: “Gala”, “Golden Delicious” and “Idared”. Moreover, the validity of the models was tested over several harvest seasons. In addition to metabolites of the triterpene biosynthetic pathway, the diagnostic markers were mainly wax esters. “Jonagold”, which is known to be susceptible to mutations, was the only cultivar for which an unambiguous classification of geographical origin was not possible.
Consumer Perceptions of the Canadian Salmon Sector and Their Associations with Behaviors: A Perspective from Indigenous Rights
Authors: Charlebois Sylvain , Sun Ning , Paul Ken , Robinson Isaiah , Colombo Stefanie M. , Music Janet , Saxena Swati , Gone Keshava Pallavi , Vezeau Janele
Published: April 2024

Previous studies on consumer perceptions and behaviors of salmon have often neglected Indigenous rights within the Canadian salmon sector. This study innovatively addresses this gap by integrating Indigenous rights into the current analysis, alongside considerations of sustainability practices, socio-economic impacts, and consumer motivations. Our research objectives aim to fit three consumer perceptions—environmental sustainability, economic considerations, and Indigenous rights—and to evaluate their associations, alongside perception of a price increase, socio-demographics, and consumer motivation factors, with purchasing behaviors related to Canadian salmon products. Data for this study was collected from a nationwide online survey. Responses to Question 2 and Question 35 are encoded with numerical values ranging from 1 to 5, where larger numbers indicate stronger agreement with the statement. The inclusion of methodologies such as the Graded Response Model (GRM) and Cumulative Link Models (CLM) adds another innovative dimension to this study. Our findings demonstrate how consumer profiles are associated with these four perceptions and their underlying determinants. Furthermore, the study quantifies the influence of these four perceptions on each consumer purchase behavior. The implications of these findings extend to the realm of mathematical modeling in consumer decision-making processes, offering practical insights for businesses and marketers, and emphasizing the importance of implementing regulatory frameworks and initiatives that promote sustainability, safeguard Indigenous rights, and address socio-economic disparities.
Residue Behaviors and Degradation Dynamics of Insecticides Commonly Applied to Agrocybe aegerita Mushrooms from Field to Product Processing and Corresponding Risk Assessments
Authors: Yao Qinghua , Su Desen , Zheng Yunyun , Xu Hui , Huang Minmin , Chen Meizhen , Zeng Shaoxiao
Published: April 2024

Residual pesticides in Agrocybe aegerita mushroom have emerged as a significant concern and bring much uncertainty due to processing procedures. In this study, a modified QuEChERS sample preparation procedure and UPLC-MS/MS were used to analyze the residual levels of four commonly used pesticides in A. aegerita from field to product processing. The field results showed that dissipation of these targeted chemicals was consistent with the first-order kinetics, and the half-life time ranged from 20.4 h to 47.6 h. The terminal residues of the four pesticides at harvest time ranged from 9.81 to 4412.56 μg/kg in raw mushroom. The processing factors (PFs) of clothianidin, diflubenzuron, chlorbenzuron, and pyridaben ranged from 0.119 to 0.808 for the drying process and from 0.191 to 1 for the washing process. By integrating the data from the field trials, the PFs, and the consumption survey, the chronic dietary risks of the target chemicals via A. aegerita intake ranged from 2.41 × 10−5 to 5.69 × 10−2 for children and from 6.34 × 10−6 to 1.88 × 10−2 for adults, which are considerably below the threshold of 1, indicating no unacceptable risk to consumers in the Fujian province of China. This research offers foundational data for appropriate use and the maximum residue limit (MRL) establishment for these four insecticides in A. aegerita.
Multi-Scale Research on the Mechanisms of Soil Arching Development and Degradation in Granular Materials with Different Relative Density
Authors: Liang Luju , Cheng Yi Pik , Fan Xiaozhen , Ding Zhi , Xu Changjie
Published: April 2024

Soil arching is significantly influenced by relative density, while its mechanisms have barely been analyzed. A series of DEM numerical simulations of the classical trapdoor test were carried out to investigate the multi-scale mechanisms of arching development and degradation in granular materials with different relative density. For analysis, the granular assembly was divided into three zones according to the particle vertical displacement normalized by the trapdoor displacement δ. The results show that before the maximum arching state (corresponding to the minimum arching ratio), contact forces between particles in a specific zone (where the vertical displacement of particles is larger than 0.1δ but less than 0.9δ) increase rapidly and robust arched force chains with large particle contact forces are generated. The variation in contact forces and force chains becomes more obvious as the sample porosity decreases. As a result, soil arching generated in a denser particle assembly is stronger, and the minimum value of the arching ratio is increased with the sample porosity. After the maximum arching state, the force chains in this zone are degenerated gradually, leading to a decrease in particle contact forces in microscale and an increase in the arching ratio in macroscale. The recovery of the arching ratio after the minimum value is also more significant in simulations with a larger relative density, as the degeneration of contact force chains is more obvious in denser samples. These results indicate the importance of contact force chain stabilities in specific zones for improving soil arching in engineering practice.
Flattened Galaxy Rotation Curves in the Exochronous Metric
Authors: Booth Robin
Published: April 2024

We examine some of the consequences of the Exochronous (timeless) metric and the associated ΣGR cosmological model for the formation of galaxies, and, in particular, their characteristic rotation curves. We show how the cumulative curvature from the multiple spatial hypersurfaces in this model leads to a modified version of the Poisson equation, in which the gravitational potential is computed over 4D space. Using this new form of the Poisson equation, we derive an analytic expression for gravitational potential as a function of radial distance for a uniform gas cloud undergoing gravitational collapse. We show that this results in a radial velocity profile that provides an excellent fit with commonly observed galaxy rotation curves, and hence fully accounts for the effects previously ascribed to dark matter. An expression can be derived for the equivalent matter density profile corresponding to the ΣGR gravitational potential, from which it is evident that this is very similar in form to the well-known Navarro–Frenk–White profile. As a further illustration of the consequences of adopting the Exochronous metric, we show how the principle can readily be incorporated into particle-mesh N-body simulations of large-scale structure evolution, using a relaxation solver for the solution to the Poisson equation and the evolution of the gravitational potential. Examples of the use of this simulation model are shown for the following cases: (a) the initial evolution of a large-scale structure, and (b) galaxy formation from a gravitationally collapsing gas cloud. In both cases, it is possible to directly visualise the build-up of the gravitational potential in 3D space as the simulation evolves and note how this corresponds to what is currently assumed to be dark matter.
Rhodotorula mucilaginosa Fungemia in an Infected Biloma Patient Following a Traumatic Liver Injury
Authors: Mokhtar Mohammad Nizam , Rahman Raha Abdul , Abdullah Farah Hanim , Azaharuddin Izzuddin , Izaham Azarinah , Ding Chuan Hun
Published: April 2024

Rhodotorula mucilaginosa fungemia is rare and highly resistant to antifungal therapy. We herein report a case involving a 31-year-old male admitted after a high-velocity road traffic accident. He sustained a grade IV liver injury with right hepatic vein thrombosis, which necessitated an urgent laparotomy. Post-operatively, repeated imaging of the abdomen revealed the presence of a biloma. Percutaneous subdiaphragmatic drainage was carried out but appeared ineffective, prompting a second surgery for an urgent hemi-hepatectomy. The patient was then nursed in the intensive care unit (ICU); however, during his stay in the ICU, he became more sepsis, which was evident by worsening ventilatory support and a rise in septic parameters from the biochemistry parameters. Despite intravenous piperacillin–tazobactam and fluconazole, his septic parameters did not improve and a full septic workup was conducted and was found to be positive for Rhodotorula mucilaginosa from the blood cultures. After discussion with the infectious disease physicians and clinical microbiologists, it was decided to initiate a course of intravenous meropenem and amphotericin B based on minimum inhibitory concentration (MIC) values, considering the patient’s extended ICU stay and catheter use. Eventually, after successfully weaning off mechanical ventilation, the patient was discharged from ICU care. This case underscores the necessity of individualized approaches, combining timely imaging, appropriate drainage techniques, and tailored treatments to optimize outcomes for such intricate post-traumatic complications.
Work-Related Musculoskeletal Injury Rates, Risk Factors, and Ergonomics in Different Endoscopic Specialties: A Review
Authors: Bessone Veronica , Roppenecker Daniel B. , Adamsen Sven
Published: April 2024

Endoscopy-related musculoskeletal injuries (ERIs) are frequent among gastrointestinal, pulmonary, nasal, and urologic endoscopists, impacting the healthcare system. The present review aims to compare the ERI rates, risk factors, and ergonomic recommendations in the different endoscopic fields. A review was conducted using PubMed and Cochrane Library for articles based on surveys and published until 10 January 2024. Demographic, work, and ERI data from 46 publications were included, covering 10,539 responders. The ERI incidence ranged between 14% and 97%, highlighting the need of intervention independent of the specialties. The neck, back, and shoulder were the most frequent ERI locations, while gender, age, years of experience, and procedure volume the most common risk factors. Ergonomic recommendations suggest concentrating on endoscope design changes, especially in gastrointestinal endoscopy, to increase the comfort, adaptability of the equipment in the operating room, and workflow/institutional policy changes. The inclusion of an ergonomic timeout guarantees the correct equipment positioning, the neutralisation of the endoscopist’s posture, and an indirect break between procedures. Ergonomic training to increase awareness and best practice should be promoted, also using new technologies. Future research should concentrate on intervention and comparative studies to evaluate to which extent prevention measures and newly designed equipment could reduce ERI incidence.
Physical Activity, Self-Care, and Menopausal Symptoms among Women in Al-Ahsa, Saudi Arabia: Adherence to Postmenopausal Guidelines (PMGs)
Authors: Jalal Sahbanathul Missiriya
Published: April 2024

Menopause is a physiological change in which the menstrual period permanently ends. Every woman experiences this transition in different ways between the ages of 40 and 55. Women may have menopausal symptoms as a result of low estrogen levels. Self-care is a practice which women can use to maintain their wellness. This study aimed to assess physical activity, self-care, and menopausal symptoms and their associations with selected variables. The results showed that women should adhere to postmenopausal guidelines (PMGs). A cross-sectional study was conducted among 212 menopausal women randomly selected from health centers in Al-Ahsa, Saudi Arabia. The international physical activity tool, a self-care questionnaire, and the Kupperman menopausal index scale were used to assess women’s physical activity, self-care, and menopausal symptoms, respectively. The chi-square and Pearson correlation tests were used for analysis. The women were 55.01 ± 6.87 years old, and 40.6% reached menopause between the ages of 46 and 50 years; 57.1% of the women had low physical activity, which was associated with menopausal symptoms (p < 0.022). The highest mean score (29.63 ± 5.83) was obtained for physical health, while the lowest (11.92 ± 3.58) was found in self-care screening tests. Regarding menopausal symptoms, 25.9% had mild, 69.8% had moderate, and 4.3% had severe symptoms. A significant association was found between menopausal symptoms and age, menopausal age, education, body mass index, and PMGs awareness at p < 0.001. Self-care positively correlated with menopausal symptoms (p < 0.001). Therefore, attention should be given to women’s adherence to PMGs so that they can enjoy healthier lives after menopause.
Global Perspectives on the Medicinal Implications of Green Walnut and Its Benefits: A Comprehensive Review
Authors: Mukarram Shaikh Ayaz , Wandhekar Sangram S. , Ahmed Abdelhakam Esmaeil Mohamed , Várallyay Szilvia , Pandey Vinay Kumar , József Prokisch , Bela Kovács
Published: April 2024

Green walnuts have been valued for their health and medicinal effects for many years. Green walnuts have several bioactive metabolites, including polyphenols, flavonoids, and tannins, that have antioxidant, anti-inflammatory, antimicrobial, and anticancer properties. With previous records of increased inclination towards natural or botanical sources of therapeutics, green walnuts have regained the uniqueness of therapeutic prominence globally. This comprehensive review provides a deep understanding of the use of green walnut (Juglans regia L.) around the globe and shows its potential pharmacological benefits. It explores the different therapeutic and medicinal uses of green walnuts, including their historical use in traditional medicine throughout different continents and regions, including Asia, Europe, America, Africa, and the Middle East. This review also provides the substantial studies conducted on green walnuts, which investigate the bioactive substances found in various parts of the walnut tree, including the kernel, shell, leaves, shoots, bark, and roots, indulging in their health advantages. It also diversifies the antibacterial, antioxidant, anti-inflammatory, cardiovascular, and antihypertensive activities of the green walnut, which clearly shows the prospective use of the walnut in nutraceutical and medicinal applications. This review continues by emphasizing the necessity for further research to focus on and attract global researchers to aim for a detailed understanding of the pharmacological implications of green walnuts and enhance their use in traditional and modern medicine.
Olive Anthracnose in Portugal Is Still Mostly Caused by Colletotrichum nymphaeae, but C. acutatum Is Spreading and C. alienum and C. cigarro Are Reported for the First Time
Authors: Cabral Ana , Nascimento Teresa , Azinheira Helena G. , Loureiro Andreia , Talhinhas Pedro , Oliveira Helena
Published: April 2024

Olive anthracnose, caused by Colletotrichum fungi, is responsible for major fruit yield losses and poor olive oil quality worldwide. In the Mediterranean basin, some Colletotrichum spp. appear to be replacing others, possibly due to climate change and modification in cultural systems. To update the situation in Portugal, 525 olive groves were surveyed throughout the country over two years, revealing a decrease in disease incidence, associated with scarcer rainfall and new cropping systems using less susceptible cultivars. A collection of 212 isolates was obtained, and phylogenetic analyses using a multi-locus sequencing approach (five and six loci in the acutatum and gloeosporioides species complex, respectively) revealed the presence of seven Colletotrichum species within the collection. Compared to surveys conducted in the first decade of the 21st century, the species composition of olive anthracnose pathogens in Portugal remains mostly unchanged, with C. nymphaeae as the prevalent species, followed by C. godetiae, but with C. acutatum geographically expanding and with C. alienum and C. cigarro being reported for the first time as causal agents of olive anthracnose in Portugal. A close attention to pathogen population shifts, in the context of climate change and modification of cultivation systems, is fundamental for anticipating plant protection measures.
Sweet Cherry Fruit Firmness Evaluation Using Compression Distance Methods
Authors: Karageorgiadou Maria , Rodovitou Maria , Nasiopoulou Elpida , Titeli Vaia Styliani , Michailidis Michail
Published: April 2024

Flesh firmness in sweet cherries is determined using the measurement of normalized deformation force, i.e., determining the required force for a distance equal to 5 or 10% of the diameter of the cherries per millimeter. However, a firmness method involving a defined distance is quite simple and suitable for easy applications. Hence, our study focuses on the impact of fruit physiology under various and fixed distances. To assess the firmness evaluation, two sweet cherry cultivars (Canada Giant and Regina) were selected and subjected to three different levels of compression distance equal to 1%, 5%, 10% of the fruit’s small thickness dimension along with a consistent compression distance of 0.16 mm. There was a strong correlation between panelists’ preferences and the fruit that had been subjected to both a 1% deformation force and a fixed distance of 0.16 mm within each cultivar. Physiological traits, membrane integrity, and the metabolome of the fruit in these categories were mostly unaffected by the control (0%), or 1%, deformation force, as shown by clustering and PCA analysis. The control and 1% deformation force groups showed similar patterns, contrary to those of the 5% and 10% deformation force groups. Given these considerations, a fixed distance of 0.16 mm and a minimal 1% deformation force possess the potential to be employed and implemented for monitoring the firmness of sweet cherries during postharvest preservation.
A Simulation–Optimization Model for Optimal Aquifer Remediation, Using Genetic Algorithms and MODFLOW
Authors: Kontos Yiannis Ν.
Published: April 2024

This paper investigates the optimal remediation process in an aquifer using Modflow 6 software and genetic algorithms. A theoretical confined aquifer has been polluted over a long period of time by unnoticed leakage in a pipeline conveying leachate from an adjacent landfill to a wastewater treatment plant. When the extended leakage and groundwater pollution are discovered, the optimal planning of the remediation strategy is investigated using the pump-and-treat method or/and hydrodynamic control of the pollution. The practical goal is to find the optimal locations and flow rates of two additional pumping wells, which will pump the polluted water or/and control pollution, protecting an existing drinking water pumping well, securing its fully operational mode even during the remediation process with the minimum possible cost, simply represented by the pumped water volume of the additional wells. The remediation process is considered complete when the maximum concentration in the aquifer drops below a certain limit. The Modflow software (handled by the Flopy Python package) simulates the flow field and advective–dispersive mass transport, and a genetic algorithm is used as the optimization tool. The coupled simulation–optimization model, Modflow-GA, complemented by a sophisticated post-processing results analysis, provides optimal and alternate sub-optimal remediation strategies for the decision makers to select from.
Synergistic Effect of Dietary Supplementation with Sodium Butyrate, β-Glucan and Vitamins on Growth Performance, Cortisol Level, Intestinal Microbiome and Expression of Immune-Related Genes in Juvenile African Catfish (Clarias gariepinus)
Authors: Arciuch-Rutkowska Martyna , Nowosad Joanna , Gil Łukasz , Czarnik Urszula , Kucharczyk Dariusz
Published: April 2024

The effect of dietary supplementation with sodium butyrate, β-glucan and vitamins (A, D3, E, K, C) on breeding indicators and immune parameters of juvenile African catfish was examined. The fish were fed with unenriched (group C) and enriched feed with a variable proportion of sodium butyrate/β-glucan, and constant content of vitamins (W1–W3). After the experiment, blood and the middle gut were collected. The microbiome of the gut was determined using Next Generation Sequencing (NGS). Liver tissue was collected for determination of expression of immune-related genes (HSP70, IL-1β, TNFα). W2 and W3 were characterized by the most favorable values of breeding indicators (p < 0.05). The highest blood cortisol concentration was in group C (71.25 ± 10.45 ng/mL), and significantly the lowest in W1 (46.03 ± 7.01 ng/ mL) (p < 0.05). The dominance of Cetobacterium was observed in all study groups, with the largest share in W3 (65.25%) and W1 (61.44%). Gene expression showed an increased number of HSP70 genes in W1. IL-1β and TNFα genes peaked at W3. The W3 variant turns out to be the most beneficial supplementation, due to the improvement of breeding and immunological parameters. The data obtained can be used to create a preparation for commercial use in the breeding of this species.
Homologous Recombination Repair Deficiency in Metastatic Prostate Cancer: New Therapeutic Opportunities
Authors: Piombino Claudia , Pipitone Stefania , Tonni Elena , Mastrodomenico Luciana , Oltrecolli Marco , Tchawa Cyrielle , Matranga Rossana , Roccabruna Sara , D’Agostino Elisa , Pirola Marta , Bacchelli Francesca , Baldessari Cinzia , Baschieri Maria Cristina , Dominici Massimo , Sabbatini Roberto , Vitale Maria Giuseppa
Published: April 2024

More than 20% of metastatic prostate cancer carries genomic defects involving DNA damage repair pathways, mainly in homologous recombination repair-related genes. The recent approval of olaparib has paved the way to precision medicine for the treatment of metastatic prostate cancer with PARP inhibitors in this subset of patients, especially in the case of BRCA1 or BRCA2 pathogenic/likely pathogenic variants. In face of this new therapeutic opportunity, many issues remain unsolved. This narrative review aims to describe the relationship between homologous recombination repair deficiency and prostate cancer, the techniques used to determine homologous recombination repair status in prostate cancer, the crosstalk between homologous recombination repair and the androgen receptor pathway, the current evidence on PARP inhibitors activity in metastatic prostate cancer also in homologous recombination repair-proficient tumors, as well as emerging mechanisms of resistance to PARP inhibitors. The possibility of combination therapies including a PARP inhibitor is an attractive option, and more robust data are awaited from ongoing phase II and phase III trials outlined in this manuscript.
An In Vitro Study on the Cytotoxic, Antioxidant, and Antimicrobial Properties of Yamogenin—A Plant Steroidal Saponin and Evaluation of Its Mechanism of Action in Gastric Cancer Cells
Authors: Stefanowicz-Hajduk Justyna , Graczyk Piotr , Hering Anna , Gucwa Magdalena , Nowak Anna , Hałasa Rafał
Published: April 2024

Yamogenin is a steroidal saponin occurring in plant species such as Asparagus officinalis, Dioscorea collettii, Trigonella foenum-graecum, and Agave sp. In this study, we evaluated in vitro cytotoxic, antioxidant, and antimicrobial properties of yamogenin. The cytotoxic activity was estimated on human colon cancer HCT116, gastric cancer AGS, squamous carcinoma UM-SCC-6 cells, and human normal fibroblasts with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The amount of apoptotic and dead AGS cells after treatment with yamogenin was estimated with flow cytometry. Also, in yamogenin-treated AGS cells we investigated the reactive oxygen species (ROS) production, mitochondrial membrane depolarization, activity level of caspase-8 and -9, and gene expression at mRNA level with flow cytometry, luminometry, and RT-PCR, respectively. The antioxidant properties of yamogenin were assessed with DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays. The antimicrobial potential of the compound was estimated on Staphylococcus aureus, Bacillus cereus, Klebsiella pneumoniae, Escherichia coli, Salmonella enterica, Helicobacter pylori, Campylobacter coli, Campylobacter jejuni, Listeria monocytogenes, Lactobacillus paracasei, and Lactobacillus acidophilus bacteria strains. Yamogenin showed the strongest cytotoxic effect on AGS cells (IC50 18.50 ± 1.24 µg/mL) among the tested cell lines. This effect was significantly stronger in combinations of yamogenin with oxaliplatin or capecitabine than for the single compounds. Furthermore, yamogenin induced ROS production, depolarized mitochondrial membrane, and increased the activity level of caspase-8 and -9 in AGS cells. RT-PCR analysis revealed that this sapogenin strongly up-regulated TNFRSF25 expression at the mRNA level. These results indicate that yamogenin induced cell death via the extrinsic and intrinsic way of apoptosis. Antioxidant study showed that yamogenin had moderate in vitro potential (IC50 704.7 ± 5.9 µg/mL in DPPH and 631.09 ± 3.51 µg/mL in ABTS assay) as well as the inhibition of protein denaturation properties (with IC50 1421.92 ± 6.06 µg/mL). Antimicrobial test revealed a weak effect of yamogenin on bacteria strains, the strongest one being against S. aureus (with MIC value of 350 µg/mL). In conclusion, yamogenin may be a potential candidate for the treatment and prevention of gastric cancers.
A Pilot Immunohistochemical Study Identifies Hedgehog Pathway Expression in Sinonasal Adenocarcinoma
Authors: Leović Matko , Jakovčević Antonija , Mumlek Ivan , Zagorac Irena , Sabol Maja , Leović Dinko
Published: April 2024

Tumors of the head and neck, more specifically the squamous cell carcinoma, often show upregulation of the Hedgehog signaling pathway. However, almost nothing is known about its role in the sinonasal adenocarcinoma, either in intestinal or non-intestinal subtypes. In this work, we have analyzed immunohistochemical staining of six Hedgehog pathway proteins, sonic Hedgehog (SHH), Indian Hedgehog (IHH), Patched1 (PTCH1), Gli family zinc finger 1 (GLI1), Gli family zinc finger 2 (GLI2), and Gli family zinc finger 3 (GLI3), on 21 samples of sinonasal adenocarcinoma and compared them with six colon adenocarcinoma and three salivary gland tumors, as well as with matching healthy tissue, where available. We have detected GLI2 and PTCH1 in the majority of samples and also GLI1 in a subset of samples, while GLI3 and the ligands SHH and IHH were generally not detected. PTCH1 pattern of staining shows an interesting pattern, where healthy samples are mostly positive in the stromal compartment, while the signal shifts to the tumor compartment in tumors. This, taken together with a stronger signal of GLI2 in tumors compared to non-tumor tissues, suggests that the Hedgehog pathway is indeed activated in sinonasal adenocarcinoma. As Hedgehog pathway inhibitors are being tested in combination with other therapies for head and neck squamous cell carcinoma, this could provide a therapeutic option for patients with sinonasal adenocarcinoma as well.
Effect of Poria cocos Terpenes: Verifying Modes of Action Using Molecular Docking, Drug-Induced Transcriptomes, and Diffusion Network Analyses
Authors: Park Musun , Yi Jin-Mu , Kim No Soo , Lee Seo-Young , Lee Haeseung
Published: April 2024

We characterized the therapeutic biological modes of action of several terpenes in Poria cocos F.A Wolf (PC) and proposed a broad therapeutic mode of action for PC. Molecular docking and drug-induced transcriptome analysis were performed to confirm the pharmacological mechanism of PC terpene, and a new analysis method, namely diffusion network analysis, was proposed to verify the mechanism of action against Alzheimer’s disease. We confirmed that the compound that exists only in PC has a unique mechanism through statistical-based docking analysis. Also, docking and transcriptomic analysis results could reflect results in clinical practice when used complementarily. The detailed pharmacological mechanism of PC was confirmed by constructing and analyzing the Alzheimer’s disease diffusion network, and the antioxidant activity based on microglial cells was verified. In this study, we used two bioinformatics approaches to reveal PC’s broad mode of action while also using diffusion networks to identify its detailed pharmacological mechanisms of action. The results of this study provide evidence that future pharmacological mechanism analysis should simultaneously consider complementary docking and transcriptomics and suggest diffusion network analysis, a new method to derive pharmacological mechanisms based on natural complex compounds.
Review on Preparation of Perovskite Solar Cells by Pulsed Laser Deposition
Authors: Lu Xinyu , Fan Xingjian , Zhang Hao , Xu Qingyu , Ijaz Mohsin
Published: April 2024

Pulsed laser deposition (PLD) is a simple and extremely versatile technique to grow thin films and nanomaterials from a wide variety of materials. Compared to traditional fabrication methods, PLD is a clean physical vapour deposition approach that avoids complicated chemical reactions and by-products, achieving a precise stochiometric transfer of the target material onto the substrate and providing control over the film thickness. Halide perovskite materials have attracted extensive attention due to their excellent photoelectric and photovoltaic properties. In this paper, we present an overview of the fundamental and practical aspects of PLD. The properties and preparation methods of the halide perovskite materials are briefly discussed. Finally, we will elaborate on recent research on the preparation of perovskite solar cells by PLD, summarize the advantages and disadvantages of the PLD preparation, and prospect the all-vacuum PLD-grown solar cells in a full solar cell structure.
A Concept for a Multipurpose Time-of-Flight Neutron Reflectometer at Compact Neutron Sources
Authors: Erhan Raul-Victor , de Haan Victor-Otto , Frommen Christoph , Knudsen Kenneth Dahl , Llamas-Jansa Isabel , Hauback Bjørn Christian
Published: April 2024

The design of a time-of-flight neutron reflectometer proposed for the new generation of compact neutron sources is presented. The reflectometer offers the possibility to use spin-polarized neutrons. The reflectometer design presented here takes advantage of a cold neutron source and uses neutrons with wavelengths in the range of 2–15 Å for the unpolarized mode. In general, due to tight spatial restrictions and the need to avoid moving parts inside the beam channel, a multi-channel collimator guide and reflective neutron guide are used for the first section of the instrument. This enables definition of the desired wavelength band and easy selection of one out of three different Q-resolutions. A low background for the collimator system and the reflectometer is ensured by employing a tangential beam channel and an in-channel sapphire filter. The second section is the time-of-flight (TOF) system, which uses a double-disk neutron chopper followed by polarization elements, the sample environment and the neutron detector system. Monte Carlo simulations and neutron beamline intensity measurements are presented. The design considerations are adoptable for neutron sources where space is limited and sections of the instrument are in a high-radiation environment.
Experimental Research on an Afterburner System Fueled with Hydrogen–Methane Mixtures
Authors: Florean Florin Gabriel , Mangra Andreea , Enache Marius , Deaconu Marius , Ciobanu Razvan , Carlanescu Razvan
Published: April 2024

A new afterburner installation is proposed, fueled with pure hydrogen (100%H2) or hydrogen–methane mixtures (60% H2 + 40% CH4, 80% H2 + 20% CH4) for use in cogeneration applications. Two prototypes (P1 and P2) with the same expansion angle (45 degrees) were developed and tested. P1 was manufactured by the classic method and P2 by additive manufacturing. Both prototypes were manufactured from Inconel 625. During the tests, analysis of flue gas (CO2, CO, and NO concentration), PIV measurements, and noise measurements were conducted. The flue gas analysis emphasizes that the behavior of the two tested prototypes was very similar. For all three fuels used, the CO2 concentration levels were slightly lower in the case of the additive-manufactured prototype P2. The CO concentration levels were significantly higher in the case of the additive-manufactured prototype P2 when 60% H2/40% CH4 and 80% H2/20% CH4 mixtures were used as fuel. When pure H2 was used as fuel, the measured data suggest that no additional CO was produced during the combustion process, and the level of CO was similar to that from the Garrett micro gas turbine in all five measuring points. The NO emissions gradually decreased as the percentage of H2 in the fuel mixture increased. The NO concentration was significantly lower in the case of the additive-manufactured prototype (P2) in comparison with the classic manufactured prototype (P1). Examining the data obtained from the PIV measurements of the flow within the mixing region shows that the highest axial velocity component value on the centerline was measured for the P1 prototype. The acoustic measurements showed that a higher H2 concentration led to a reduction in noise of approximately 1.5 dB for both afterburner prototypes. The outcomes reveal that the examined V-gutter flame holder prototype flow was smooth, without any perpendicular oscillations, without chaotic motions or turbulent oscillations to the flow direction, across all tested conditions, keeping constant thermal power.
Post-Blink Blur Time—A Simple Test to Detect Dry Eye-Related Visual Disturbances
Authors: Petriček Igor , Lešin Gaćina Dina , Tomić Martina , Bulum Tomislav , Bešlić Iva , Vidas Pauk Sania
Published: April 2024

Background: Dry eye disease (DED) stands out as one of the most common eye conditions encountered in clinical settings. This study aimed to determine the diagnostic ability and feasibility of post-blink blur time (PBBT) in detecting patients with DED symptoms. Methods: The study included 200 subjects, 100 with and 100 without DED symptoms defined by the Schein questionnaire, who underwent assessment of DED signs [visual acuity, PBBT, conjunctival hyperemia, lid-parallel conjunctival folds—LIPCOF, tear film break-up time—TBUT, fluorescein corneal staining, and meibum score]. Results: DED subjects had a lower PBBT than controls (p < 0.001), with subjective (6 (1–45) s vs. 8 (1–70) s) and objective (6 (2–33) s vs. 8 (2–50) s) PBBT measurements being similar between repeats. The correlations between subjective and objective PBBT measurements were significantly positive (R = 0.873, p < 0.001). Subjective PBBT was negatively related to the Schein questionnaire (R = −0.217, p = 0.002), conjunctival hyperemia (R = −0.105, p = 0.035), and corneal staining (R = −0.153, p = 0.031), while positively related to the TBUT (R = 0.382, p < 0.001) and meibum score (R = 0.106, p = 0.033). Logistic regression analysis showed DED symptoms were significantly associated with subjective PBBT (AOR 0.91, p = 0.001), TBUT (AOR 0.79, p < 0.001), meibum score (AOR 0.65, p = 0.008), LIPCOF (AOR 1.18, p = 0.002) and corneal staining (AOR 1.14, p = 0.028). Conclusions: Subjective self-reported PBBT is a reliable and non-invasive screening test for evaluating time-wise changes in visual acuity and detecting a tear film dysfunction.
Bioactive Dressing: A New Algorithm in Wound Healing
Authors: Polverino Gianmarco , Russo Francesca , D’Andrea Francesco
Published: April 2024

Wound management presents a significant global challenge, necessitating a comprehensive understanding of wound care products and clinical expertise in selecting dressings. Bioactive dressings (BD) represent a diverse category of dressings, capable of influencing wound healing through various mechanisms. These dressings, including honey, hyaluronic acid, collagen, alginates, and polymers enriched with polyhexamethylene biguanide, chitin, and chitosan derivatives, create a conducive environment for healing, promoting moisture balance, pH regulation, oxygen permeability, and fluid management. Interactive dressings further enhance targeted action by serving as substrates for bioactive agents. The continuous evolution of BDs, with new products introduced annually, underscores the need for updated knowledge in wound care. To facilitate dressing selection, a practical algorithm considers wound exudate, infection probability, and bleeding, guiding clinicians through the process. This algorithm aims to optimize wound care by ensuring the appropriate selection of BDs tailored to individual patient needs, ultimately improving outcomes in wound management.
Acute Lung Injury after Cardiopulmonary Resuscitation: A Narrative Review
Authors: Marchese Giuseppe , Bungaro Elisabetta , Magliocca Aurora , Fumagalli Francesca , Merigo Giulia , Semeraro Federico , Mereto Elisa , Babini Giovanni , Roman-Pognuz Erik , Stirparo Giuseppe , Cucino Alberto , Ristagno Giuseppe
Published: April 2024

Although cardiopulmonary resuscitation (CPR) includes lifesaving maneuvers, it might be associated with a wide spectrum of iatrogenic injuries. Among these, acute lung injury (ALI) is frequent and yields significant challenges to post-cardiac arrest recovery. Understanding the relationship between CPR and ALI is determinant for refining resuscitation techniques and improving patient outcomes. This review aims to analyze the existing literature on ALI following CPR, emphasizing prevalence, clinical implications, and contributing factors. The review seeks to elucidate the pathogenesis of ALI in the context of CPR, assess the efficacy of CPR techniques and ventilation strategies, and explore their impact on post-cardiac arrest outcomes. CPR-related injuries, ranging from skeletal fractures to severe internal organ damage, underscore the complexity of managing post-cardiac arrest patients. Chest compression, particularly when prolonged and vigorous, i.e., mechanical compression, appears to be a crucial factor contributing to ALI, with the concept of cardiopulmonary resuscitation-associated lung edema (CRALE) gaining prominence. Ventilation strategies during CPR and post-cardiac arrest syndrome also play pivotal roles in ALI development. The recognition of CPR-related lung injuries, especially CRALE and ALI, highlights the need for research on optimizing CPR techniques and tailoring ventilation strategies during and after resuscitation.