bluuebunny/crossref_metadata_2025 huggingface dataset:
The Human Vulvar Microbiome: A Systematic Review
Authors: Pagan Lisa , Ederveen Roos A. M. , Huisman Bertine W. , Schoones Jan W. , Zwittink Romy D. , Schuren Frank H. J. , Rissmann Robert , Piek Jurgen M. J. , van Poelgeest Mariëtte I. E.
Published: December 2021

The link between cancer and the microbiome is a fast-moving field in research. There is little knowledge on the microbiome in ((pre)malignant) conditions of the vulvar skin. This systematic review aims to provide an overview of the literature regarding the microbiome composition of the healthy vulvar skin and in (pre)malignant vulvar disease. This study was performed according to the PRISMA guidelines. A comprehensive, electronic search strategy was used to identify original research articles (updated September 2021). The inclusion criteria were articles using culture-independent methods for microbiome profiling of the vulvar region. Ten articles were included. The bacterial composition of the vulva consists of several genera including Lactobacillus, Corynebacterium, Staphylococcus and Prevotella, suggesting that the vulvar microbiome composition shows similarities with the corresponding vaginal milieu. However, the vulvar microbiome generally displayed higher diversity with commensals of cutaneous and fecal origin. This is the first systematic review that investigates the relationship between microbiome and vulvar (pre)malignant disease. There are limited data and the level of evidence is low with limitations in study size, population diversity and methodology. Nevertheless, the vulvar microbiome represents a promising field for exploring potential links for disease etiology and targets for therapy.
Polysaccharide Hydrogels for the Protection of Dairy-Related Microorganisms in Adverse Environmental Conditions
Authors: Osojnik Črnivec Ilja Gasan , Neresyan Tigran , Gatina Yuliana , Kolmanič Bučar Vid , Skrt Mihaela , Dogša Iztok , Bogovič Matijašić Bojana , Kulikova Irina , Lodygin Aleksei , Poklar Ulrih Nataša
Published: December 2021

Adverse environmental conditions are severely limiting the use of microorganisms in food systems, such as probiotic delivery, where low pH causes a rapid decrease in the survival of ingested bacteria, and mixed-culture fermentation, where stepwise changes and/or metabolites of individual microbial groups can hinder overall growth and production. In our study, model probiotic lactic acid bacteria (L. plantarum ATCC 8014, L. rhamnosus GG) and yeasts native to dairy mixed cultures (K. marxianus ZIM 1868) were entrapped in an optimized (cell, alginate and hardening solution concentration, electrostatic working parameters) Ca-alginate system. Encapsulated cultures were examined for short-term survival in the absence of nutrients (lactic acid bacteria) and long-term performance in acidified conditions (yeasts). In particular, the use of encapsulated yeasts in these conditions has not been previously examined. Electrostatic manufacturing allowed for the preparation of well-defined alginate microbeads (180–260 µm diameter), high cell-entrapment (95%) and viability (90%), and uniform distribution of the encapsulated cells throughout the hydrogel matrix. The entrapped L. plantarum maintained improved viabilities during 180 min at pH 2.0 (19% higher when compared to the free culture), whereas, L. rhamnosus appeared to be less robust. The encapsulated K. marxianus exhibited double product yields in lactose- and lactic acid-modified MRS growth media (compared to an unfavorable growth environment for freely suspended cells). Even within a conventional encapsulation system, the pH responsive features of alginate provided superior protection and production of encapsulated yeasts, allowing several applications in lacto-fermented or acidified growth environments, further options for process optimization, and novel carrier design strategies based on inhibitor charge expulsion.
Assessment of Platelet Reactivity and Inflammatory Markers in Coronary Artery Bypass Graft Patients Treated with Acetylsalicylic Acid with Flavonoid Supplementation
Authors: Siennicka Aldona , Kłysz Magdalena , Adamska Monika , Chełstowski Kornel , Biskupski Andrzej , Jastrzębska Maria
Published: December 2021

The recommended pharmacological therapy for patients with coronary artery disease (CAD) treated by coronary artery bypass grafting (CABG) is acetylsalicylic acid (ASA). To improve the antiplatelet effect, supplementation with flavonoids is also recommended. The aim of this study was to estimate anti-aggregation properties of diosmin, in combination with ASA, pre- and postoperatively and assess the relationship of this therapy with inflammatory processes in CAD patients undergoing CABG. The study patients (n = 26) took diosmin (1000 mg/day); the control patients (n = 27) took a placebo. The therapeutic period for taking diosmin was from at least 30 days before to 30 days after CABG. All patients also took 75 mg/day ASA. Platelet aggregation and IL-6, CRP, and fibrinogen concentrations were determined before and 30 days after surgery. Results showed that diosmin did not enhance the anti-aggregation effect of ASA at any assessment time. However, there was a stronger anti-aggregation effect 30 days after surgery that was diosmin independent and was associated with acute-phase markers in the postoperative period. Increased levels of inflammatory markers in the late phase of the postoperative period may provide an unfavorable prognostic factor in long-term follow-up, which should prompt the use of stronger antiplatelet therapy in patients after CABG.
Self-Diffusion in Simple Liquids as a Random Walk Process
Authors: Khrapak Sergey A.
Published: December 2021

It is demonstrated that self-diffusion in dense liquids can be considered a random walk process; its characteristic length and time scales are identified. This represents an alternative to the often assumed hopping mechanism of diffusion in the liquid state. The approach is illustrated using the one-component plasma model.
Phosphonodithioester–Amine Coupling as a Key Reaction Step for the Design of Cationic Amphiphiles Used for Gene Delivery
Authors: Khalil Montassar , Hocquigny Alexis , Berchel Mathieu , Montier Tristan , Jaffrès Paul-Alain
Published: December 2021

A convergent synthesis of cationic amphiphilic compounds is reported here with the use of the phosphonodithioester–amine coupling (PAC) reaction. This versatile reaction occurs at room temperature without any catalyst, allowing binding of the lipid moiety to a polar head group. This strategy is illustrated with the use of two lipid units featuring either two oleyl chains or two-branched saturated lipid chains. The final cationic amphiphiles were evaluated as carriers for plasmid DNA delivery in four cell lines (A549, Calu3, CFBE and 16HBE) and were compared to standards (BSV36 and KLN47). These new amphiphilic derivatives, which were formulated with DOPE or DOPE-cholesterol as helper lipids, feature high transfection efficacies when associated with DOPE. The highest transfection efficacies were observed in the four cell lines at low charge ratios (CR = 0.7, 1 or 2). At these CRs, no toxic effects were detected. Altogether, this new synthesis scheme using the PAC reaction opens up new possibilities for investigating the effects of lipid or polar head groups on transfection efficacies.
Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics
Authors: Kalasariya Haresh S. , Patel Nikunj B. , Yadav Akanksha , Perveen Kahkashan , Yadav Virendra Kumar , Munshi Faris M. , Yadav Krishna Kumar , Alam Shamshad , Jung You-Kyung , Jeon Byong-Hun
Published: December 2021

Cosmetic industries are highly committed to finding natural sources of functional active constituents preferable to safer materials to meet consumers’ demands. Marine macroalgae have diversified bioactive constituents and possess potential benefits in beauty care products. Hence, the present study was carried out to characterize the biochemical profile of marine macroalga Chaetomorpha crassa by using different techniques for revealing its cosmetic potentials. In results, the FTIR study characterized the presence of different bioactive functional groups that are responsible for many skin-beneficial compounds whereas six and fifteen different important phycocompounds were found in GCMS analysis of ethanolic and methanolic extracts, respectively. In the saccharide profile of C. crassa, a total of eight different carbohydrate derivatives were determined by the HRLCMS Q-TOF technique, which showed wide varieties of cosmetic interest. In ICP AES analysis, Si was found to be highest whereas Cu was found to be lowest among other elements. A total of twenty-one amino acids were measured by the HRLCMS-QTOF technique, which revealed the highest amount of the amino acid, Aspartic acid (1207.45 nmol/mL) and tyrosine (106.77 nmol/mL) was found to be the lowest in amount among other amino acids. Their cosmetic potentials have been studied based on previous research studies. The incorporation of seaweed-based bioactive components in cosmetics has been extensively growing due to its skin health-promoting effects.
Enriching the Arsenal of Pharmacological Tools against MICAL2
Authors: Barravecchia Ivana , Barresi Elisabetta , Russo Camilla , Scebba Francesca , De Cesari Chiara , Mignucci Valerio , De Luca Davide , Salerno Silvia , La Pietra Valeria , Giustiniano Mariateresa , Pelliccia Sveva , Brancaccio Diego , Donati Greta , Da Settimo Federico , Taliani Sabrina , Angeloni Debora , Marinelli Luciana
Published: December 2021

Molecule interacting with CasL 2 (MICAL2), a cytoskeleton dynamics regulator, are strongly expressed in several human cancer types, especially at the invasive front, in metastasizing cancer cells and in the neo-angiogenic vasculature. Although a plethora of data exist and stress a growing relevance of MICAL2 to human cancer, it is worth noting that only one small-molecule inhibitor, named CCG-1423 (1), is known to date. Herein, with the aim to develop novel MICAL2 inhibitors, starting from CCG-1423 (1), a small library of new compounds was synthetized and biologically evaluated on human dermal microvascular endothelial cells (HMEC-1) and on renal cell adenocarcinoma (786-O) cells. Among the novel compounds, 10 and 7 gave interesting results in terms of reduction in cell proliferation and/or motility, whereas no effects were observed in MICAL2-knocked down cells. Aside from the interesting biological activities, this work provides the first structure–activity relationships (SARs) of CCG-1423 (1), thus providing precious information for the discovery of new MICAL2 inhibitors.
Changes in Porous Parameters of the Ion Exchanged X Zeolite and Their Effect on CO2 Adsorption
Authors: Gęsikiewicz-Puchalska Andżelika , Zgrzebnicki Michal , Michalkiewicz Beata , Kałamaga Agnieszka , Narkiewicz Urszula , Morawski Antoni W. , Wrobel Rafal
Published: December 2021

Zeolite 13X (NaX) was modified through ion-exchange with alkali and alkaline earth metal cations. The degree of ion exchange was thoroughly characterized with ICP, EDS and XRF methods. The new method of EDS data evaluation for zeolites was presented. It delivers the same reliable results as more complicated, expensive, time consuming and hazardous ICP approach. The highest adsorption capacities at 273 K and 0.95 bar were achieved for materials containing the alkali metals in the following order K < Na < Li, respectively, 4.54, 5.55 and 5.94 mmol/g. It was found that it is associated with the porous parameters of the ion-exchanged samples. The Li0.61Na0.39X form of zeolite exhibited the highest specific surface area of 624 m2/g and micropore volume of 0.35 cm3/g compared to sodium form 569 m2/g and 0.30 cm3/g, respectively. The increase of CO2 uptake is not related with deterioration of CO2 selectivity. At room temperature, the CO2 vs. N2 selectivity remains at a very high stable level prior and after ion exchange in co-adsorption process (XCO2 during adsorption 0.15; XCO2 during desorption 0.95) within measurement uncertainty. Additionally, the Li0.61Na0.39X sample was proven to be stable in the aging adsorption-desorption tests (200 sorption-desorption cycles; circa 11 days of continuous process) exhibiting the CO2 uptake decrease of about 6%. The exchange with alkaline earth metals (Mg, Ca) led to a significant decrease of SSA and micropore volume which correlated with lower CO2 adsorption capacities. Interestingly, the divalent cations cause formation of mesopores, due to the relaxation of lattice strains.
Residues R1075, D1090, R1095, and C1130 Are Critical in ADAMTS13 TSP8-Spacer Interaction Predicted by Molecular Dynamics Simulation
Authors: Wu Zhiwei , Yang Junxian , Xie Xubin , Liu Guangjian , Fang Ying , Wu Jianhua , Lin Jiangguo
Published: December 2021

ADAMTS13 (A Disintegrin and Metalloprotease with Thrombospondin type 1 repeats, member 13) cleaves von Willebrand Factor (VWF) multimers to limit the prothrombotic function of VWF. The deficiency of ADAMTS13 causes a lethal thrombotic microvascular disease, thrombotic thrombocytopenic purpura (TTP). ADAMTS13 circulates in a “closed” conformation with the distal domain associating the Spacer domain to avoid off-target proteolysis or recognition by auto-antibodies. However, the interactions of the distal TSP8 domain and the Spacer domain remain elusive. Here, we constructed the TSP8-Spacer complex by a combination of homology modelling and flexible docking. Molecular dynamics simulation was applied to map the binding sites on the TSP8 or Spacer domain. The results predicted that R1075, D1090, R1095, and C1130 on the TSP8 domain were key residues that interacted with the Spacer domain. R1075 and R1095 bound exosite-4 tightly, D1090 formed multiple hydrogen bonds and salt bridges with exosite-3, and C1130 interacted with both exosite-3 and exosite-4. Specific mutations of exosite-3 (R568K/F592Y/R660K/Y661F/Y665F) or the four key residues (R1075A/D1090A/R1095A/C1130A) impaired the binding of the TSP8 domain to the Spacer domain. These results shed new light on the understanding of the auto-inhibition of ADAMTS13.
Multiple Sclerosis, Viruses, and New Vaccines
Authors: Maple Peter A. C.
Published: December 2021

Multiple sclerosis (MS) is the most common inflammatory neurological disease in young adults, with an estimated prevalence of approximately 2 [...]
Levocarnitine Supplementation Suppresses Lenvatinib-Related Sarcopenia in Hepatocellular Carcinoma Patients: Results of a Propensity Score Analysis
Authors: Okubo Hironao , Ando Hitoshi , Nakadera Eisuke , Ikejima Kenichi , Shiina Shuichiro , Nagahara Akihito
Published: December 2021

This study investigated the inhibitory effect of levocarnitine supplementation on sarcopenia progression in hepatocellular carcinoma (HCC) patients treated with lenvatinib. We evaluated the skeletal muscle index (SMI). After propensity score matching for age, sex, modified albumin-bilirubin grade, baseline presence of sarcopenia, and branched-chain amino acid administration, we selected 17 patients who received levocarnitine supplementation after starting lenvatinib therapy and 17 propensity-score-matched patients who did not receive levocarnitine. Sarcopenia was present in 76% of the patients at baseline. Changes in baseline SMI at 6 and 12 weeks of treatment were significantly suppressed in the group with levocarnitine supplementation compared with those without (p = 0.009 and p = 0.018, respectively). While there were no significant differences in serum free carnitine levels in cases without levocarnitine supplementation between baseline and after 6 weeks of treatment (p = 0.193), free carnitine levels were significantly higher after 6 weeks of treatment compared with baseline in cases with levocarnitine supplementation (p < 0.001). Baseline SMI and changes in baseline SMI after 6 weeks of treatment were significantly correlated with free carnitine levels (r = 0.359, p = 0.037; and r = 0.345, p = 0.045, respectively). Levocarnitine supplementation can suppress sarcopenia progression during lenvatinib therapy.
Dietary Composition and Its Association with Newly Diagnosed Nonalcoholic Fatty Liver Disease and Insulin Resistance
Authors: Charatcharoenwitthaya Phunchai , Tansakul Eakchakarj , Chaiyasoot Kusuma , Bandidniyamanon Wimolrak , Charatcharoenwitthaya Natthinee
Published: December 2021

Dietary modification is essential for treating nonalcoholic fatty liver disease (NAFLD); however, the dietary components are less well defined. We enrolled 252 adults with no history of liver disease and excessive alcohol use to evaluate the relationship between macronutrients and NAFLD and insulin resistance. Participants took photographs of their meals and documented their food intake in a food diary for seven consecutive days. A dietitian estimated the type and portion size of food items and analyzed nutrients with INMUCAL-Nutrients software. Later, participants underwent transient elastography to diagnose NAFLD and blood tests to measure insulin resistance using the homeostasis model. Total energy intake and the proportion of carbohydrate, fat, and protein consumption did not differ between participants with NAFLD (n = 41) and those without NAFLD (n = 211). Using multiple logistic regression analysis, daily intake of protein < 1.0 g/kg (OR: 3.66, 95% CI: 1.41–9.52) and full-fat dairy product ≥ 50 g (OR: 0.42, 95% CI: 0.18–0.99) were associated with NAFLD. Insulin resistance was associated with a daily intake of protein < 1.0 g/kg (OR: 3.09, 95% CI: 1.59–6.05), full-fat dairy product ≥ 50 g (OR: 0.46, 95% CI: 0.25–0.82), and dietary fiber ≥ 8 g (OR: 0.41, 95% CI: 0.22–0.74). Our data show that a low protein intake increases the odds for NAFLD and insulin resistance. Contrarily, a high intake of full-fat dairy products and dietary fiber has been associated with a potential protective effect against NAFLD and insulin resistance.
Curcumin in Metabolic Health and Disease
Authors: Jabczyk Marzena , Nowak Justyna , Hudzik Bartosz , Zubelewicz-Szkodzińska Barbara
Published: December 2021

In recent years, epidemiological studies have suggested that metabolic disorders are nutritionally dependent. A healthy diet that is rich in polyphenols may be beneficial in the treatment of metabolic diseases such as polycystic ovary syndrome, metabolic syndrome, non-alcoholic fatty liver disease, cardiovascular disease, and, in particular, atherosclerosis. Curcumin is a polyphenol found in turmeric and has been reported to have antioxidant, anti-inflammatory, hepatoprotective, anti-atherosclerotic, and antidiabetic properties, among others. This review summarizes the influence of supplementation with curcumin on metabolic parameters in selected metabolic disorders.
Shorter Women Are More Dissatisfied with Their Height: An Exploration of Height Dissatisfaction in Australian Women
Authors: Perkins Thomas , Hayes Samantha , Talbot Daniel
Published: December 2021

Height dissatisfaction has recently been found to be an important contributor to body dissatisfaction in males, however, there is currently limited research examining this relationship in women. The present cross-sectional study aimed to examine the link between height and height dissatisfaction in Australian women, and the extent to which height and height dissatisfaction relate to body dissatisfaction and quality of life. Additionally, it explored the height preferences of oneself and romantic partners in Australian women. A final sample of 172 Australian women was recruited to complete an online survey relating to height preference, height dissatisfaction, muscularity, drive for thinness, and quality of life. Results showed that there was a significant difference between the reported actual height of participants and their ideal height. We also found that shorter women tended to be more dissatisfied with their height and were more likely to believe that they were treated poorly due to their height. Our study also identified that the vast majority (84%) preferred a romantic partner taller than them. Results were comparable to men aspiring to be taller than they were. Future studies should consider height dissatisfaction as a potential contributing factor to poor quality of life and negative affect.
Yttrium-90 Internal Radiation Therapy as Part of the Multimodality Treatment of Metastatic Colorectal Carcinoma
Authors: Del Rosario Michael P. , Abi-Jaoudeh Nadine , Cho May T. , Jutric Zeljka , Dayyani Farshid
Published: December 2021

About 70% of patients with metastatic colorectal carcinoma (mCRC) have liver metastases. Hepatic failure accounts for most mCRC-related deaths. Therefore, controlling liver metastases may improve outcomes. A data overview of liver-directed treatment using yttrium-90 selective internal radiation therapy (SIRT) is provided as part of a multimodality treatment. SIRT in mCRC is discussed, and the prognostic factors for patient selection are defined. Pooled analyses of three recent trials incorporating SIRT plus chemotherapy revealed subsets of patients with mCRC who might benefit from SIRT. A multidisciplinary treatment for most mCRC patients is proposed to achieve long-term survival in this cohort of patients.
A Novel Flow Cytometric Approach for the Quantification and Quality Control of Chlamydia trachomatis Preparations
Authors: Klasinc Romana , Reiter Michael , Digruber Astrid , Tschulenk Waltraud , Walter Ingrid , Kirschner Alexander , Spittler Andreas , Stockinger Hannes
Published: December 2021

Chlamydia trachomatis is an obligate intracellular pathogenic bacterium with a biphasic developmental cycle manifesting two distinct morphological forms: infectious elementary bodies (EBs) and replicative intracellular reticulate bodies (RBs). Current standard protocols for quantification of the isolates assess infectious particles by titering inclusion-forming units, using permissive cell lines, and analyzing via immunofluorescence. Enumeration of total particle counts is achieved by counting labeled EBs/RBs using a fluorescence microscope. Both methods are time-consuming with a high risk of observer bias. For a better assessment of C. trachomatis preparations, we developed a simple and time-saving flow cytometry-based workflow for quantifying small particles, such as EBs with a size of 300 nm. This included optimization of gain and threshold settings with the addition of a neutral density filter for small-particle discrimination. The nucleic acid dye SYBR® Green I (SGI) was used together with propidium iodide and 5(6)-carboxyfluorescein diacetate to enumerate and discriminate between live and dead bacteria. We found no significant differences between the direct particle count of SGI-stained C. trachomatis preparations measured by microscopy or flow cytometry (p > 0.05). Furthermore, we completed our results by introducing a cell culture-independent viability assay. Our measurements showed very good reproducibility and comparability to the existing state-of-the-art methods, indicating that the evaluation of C. trachomatis preparations by flow cytometry is a fast and reliable method. Thus, our method facilitates an improved assessment of the quality of C. trachomatis preparations for downstream applications.
Conceptualisation of an Efficient Particle-Based Simulation of a Twin-Screw Granulator
Authors: Morrissey John P. , Hanley Kevin J. , Ooi Jin Y.
Published: December 2021

Discrete Element Method (DEM) simulations have the potential to provide particle-scale understanding of twin-screw granulators. This is difficult to obtain experimentally because of the closed, tightly confined geometry. An essential prerequisite for successful DEM modelling of a twin-screw granulator is making the simulations tractable, i.e., reducing the significant computational cost while retaining the key physics. Four methods are evaluated in this paper to achieve this goal: (i) develop reduced-scale periodic simulations to reduce the number of particles; (ii) further reduce this number by scaling particle sizes appropriately; (iii) adopt an adhesive, elasto-plastic contact model to capture the effect of the liquid binder rather than fluid coupling; (iv) identify the subset of model parameters that are influential for calibration. All DEM simulations considered a GEA ConsiGma™ 1 twin-screw granulator with a 60° rearward configuration for kneading elements. Periodic simulations yielded similar results to a full-scale simulation at significantly reduced computational cost. If the level of cohesion in the contact model is calibrated using laboratory testing, valid results can be obtained without fluid coupling. Friction between granules and the internal surfaces of the granulator is a very influential parameter because the response of this system is dominated by interactions with the geometry.
Discovery of the First in Class 9-N-Berberine Derivative as Hypoglycemic Agent with Extra-Strong Action
Authors: Khvostov Mikhail V. , Gladkova Elizaveta D. , Borisov Sergey A. , Zhukova Nataliya A. , Marenina Mariya K. , Meshkova Yuliya V. , Luzina Olga A. , Tolstikova Tatijana G. , Salakhutdinov Nariman F.
Published: December 2021

Berberine is well known for its ability to reduce the blood glucose level, but its high effective dose and poor bioavailability limits its use. In this work we synthesized a new derivative of berberine, 9-(hexylamino)-2,3-methylenedioxy-10-methoxyprotoberberine chloride (SHE-196), and analyzed the profile of its hypoglycemic effects. Biological tests have shown that the substance has a very pronounced hypoglycemic activity due to increased insulin sensitivity after single and multiple dosing. In obese type 2 diabetes mellitus (T2DM) mice, it was characterized by improved glucose tolerance, decreased fasting insulin levels and sensitivity, decreased total body weight and interscapular fat mass, and increased interscapular brown fat activity. All these effects were also confirmed histologically, where a decrease in fatty degeneration of the liver, an improvement in the condition of the islets of Langerhans and a decrease in the size of fat droplets in brown adipose tissue were found. Our results indicate that 9-(hexylamino)-2,3-methylenedioxy-10-methoxyprotoberberine chloride could be the first in a new series of therapeutic agents for the treatment of diabetes mellitus.
Foam-like 3D Graphene as a Charge Transport Modifier in Zinc Oxide Electron Transport Material in Perovskite Solar Cells
Authors: Mohamed Saheed Mohamed Salleh , Mohamed Norani Muti , Mahinder Singh Balbir Singh , Wali Qamar , Saheed Mohamed Shuaib Mohamed , Jose Rajan
Published: December 2021

The effect of foam-like 3D graphene (3DG) in an electron transport material (ETM), viz. ZnO thin film, on the steady-state photoluminescence (PL), light-harvesting efficiency (LHE), photocurrent density (JSC), photovoltage (VOC), and charge transport parameters of perovskite solar cells (PSCs) are systematically investigated. The ETM is developed by spin coating a ZnO precursor solution containing varying amounts of 3DG on conducting glass substrates and appropriate annealing. A significant improvement in the photoconversion efficiency of PSCs is observed for a low concentration of 3DG in ZnO. The current–voltage and electrochemical impedance spectroscopy measurements show that the addition of 3DG enhances the VOC due to efficient electron–hole separation and charge transport compared to the pristine ZnO. These studies offer a route for further advances in enhancing the optoelectronic properties of ETM for artificial photosynthesis and photocatalysis devices.
Reaching into the N = 40 Island of Inversion with Nucleon Removal Reactions
Authors: Gade Alexandra
Published: December 2021

One ambitious goal of nuclear physics is a predictive model of all nuclei, including the ones at the fringes of the nuclear chart which may remain out of experimental reach. Certain regions of the chart are providing formidable testing grounds for nuclear models in this quest as they display rapid structural evolution from one nucleus to another or phenomena such as shape coexistence. Observables measured for such nuclei can confirm or refute our understanding of the driving forces of the evolution of nuclear structure away from stability where textbook nuclear physics has been proven to not apply anymore. This paper briefly reviews the emerging picture for the very neutron-rich Fe, Cr, and Ti isotopes within the so-called N=40 island of inversion as obtained with nucleon knockout reactions. These have provided some of the most detailed nuclear spectroscopy in very neutron-rich nuclei produced at rare-isotope facilities. The results indicate that our current understanding, as encoded in large-scale shell-model calculations, appears correct with exciting predictions for the N=40 island of inversion left to be proven in the experiment. A bright future emerges with predictions of continued shell evolution and shape coexistence out to neutron number N=50, below 78Ni on the chart of nuclei.
Alterations in the Transcriptome of Rye Plants following the Microdochium nivale Infection: Identification of Resistance/Susceptibility-Related Reactions Based on RNA-Seq Analysis
Authors: Tsers Ivan , Meshcherov Azat , Gogoleva Olga , Petrova Olga , Gogoleva Natalia , Ponomareva Mira , Gogolev Yuri , Korzun Viktor , Gorshkov Vladimir
Published: December 2021

Microdochium nivale is a progressive and devastating phytopathogen that causes different types of cereal crop and grass diseases that are poorly characterized at the molecular level. Although rye (Secale cereale L.) is one of the most resistant crops to most of the phytopathogens, it is severely damaged by M. nivale. The recent high-quality chromosome-scale assembly of rye genome has improved whole-genome studies of this crop. In the present work, the first transcriptome study of the M. nivale-infected crop plant (rye) with the detailed functional gene classification was carried out, along with the physiological verification of the RNA-Seq data. The results revealed plant reactions that contributed to their resistance or susceptibility to M. nivale. Phytohormone abscisic acid was shown to promote plant tolerance to M. nivale. Flavonoids were proposed to contribute to plant resistance to this pathogen. The upregulation of plant lipase encoding genes and the induction of lipase activity in M. nivale-infected plants revealed in our study were presumed to play an important role in plant susceptibility to the studied phytopathogen. Our work disclosed important aspects of plant-M. nivale interactions, outlined the directions for future studies on poorly characterized plant diseases caused by this phytopathogen, and provided new opportunities to improve cereals breeding and food security strategies.
Sustainability Trait Modeling of Field-Grown Switchgrass (Panicum virgatum) Using UAV-Based Imagery
Authors: Xu Yaping , Shrestha Vivek , Piasecki Cristiano , Wolfe Benjamin , Hamilton Lance , Millwood Reginald J. , Mazarei Mitra , Stewart Charles Neal
Published: December 2021

Unmanned aerial vehicles (UAVs) provide an intermediate scale of spatial and spectral data collection that yields increased accuracy and consistency in data collection for morphological and physiological traits than satellites and expanded flexibility and high-throughput compared to ground-based data collection. In this study, we used UAV-based remote sensing for automated phenotyping of field-grown switchgrass (Panicum virgatum), a leading bioenergy feedstock. Using vegetation indices calculated from a UAV-based multispectral camera, statistical models were developed for rust disease caused by Puccinia novopanici, leaf chlorophyll, nitrogen, and lignin contents. For the first time, UAV remote sensing technology was used to explore the potentials for multiple traits associated with sustainable production of switchgrass, and one statistical model was developed for each individual trait based on the statistical correlation between vegetation indices and the corresponding trait. Also, for the first time, lignin content was estimated in switchgrass shoots via UAV-based multispectral image analysis and statistical analysis. The UAV-based models were verified by ground-truthing via correlation analysis between the traits measured manually on the ground-based with UAV-based data. The normalized difference red edge (NDRE) vegetation index outperformed the normalized difference vegetation index (NDVI) for rust disease and nitrogen content, while NDVI performed better than NDRE for chlorophyll and lignin content. Overall, linear models were sufficient for rust disease and chlorophyll analysis, but for nitrogen and lignin contents, nonlinear models achieved better results. As the first comprehensive study to model switchgrass sustainability traits from UAV-based remote sensing, these results suggest that this methodology can be utilized for switchgrass high-throughput phenotyping in the field.
Effect of Selenium Application and Growth Stage at Harvest on Hydrophilic and Lipophilic Antioxidants in Lamb’s Lettuce (Valerianella locusta L. Laterr.)
Authors: Skrypnik Liubov , Styran Tatiana , Savina Tamara , Golubkina Nadezhda
Published: December 2021

Lamb’s lettuce (Valerianella locusta L. Laterr.) is a leafy green vegetable that is rich in various biological active compounds and is widely used in ready-to-eat salads. The cultivation conditions and growth stage could affect the secondary metabolism in plants and thereby modify their food value. In the present study, the effect of selenium (Se) application in various concentrations (5.0, 10.0, and 20.0 µM) on the contents of Se, phenolic compounds, vitamin C, carotenoids, chlorophylls, and antioxidant activity of hydrophilic and lipophilic extracts of lamb’s lettuce harvested at three growth stages (38, 52, and 66 days after sowing (DAS)) was studied. Se application significantly increased the Se concentration in the shoots (up to 124.4 μg g−1 dry weight), as well as the contents of chlorogenic acid, total flavonoids, total phenolics, ascorbic acid, chlorophyll b, and the antioxidant activity of hydrophilic and lipophilic extracts. A higher content of phenolic compounds and higher antioxidant activity of hydrophilic extracts was observed at the first growth stage (38 DAS). On the contrary, higher contents of lipophilic compounds (chlorophyll a, chlorophyll b, lutein, β-carotene) and higher antioxidant activity of lipophilic extracts were found for shoots harvested at later stages (52 and 66 DAS).
Comparative Study of Fire Resistance and Char Formation of Intumescent Fire-Retardant Coatings Reinforced with Three Types of Shell Bio-Fillers
Authors: Wang Feiyue , Liu Hui , Yan Long
Published: December 2021

Three types of shell bio-fillers, including eggshell (CES), conch shell (CHS) and clamshell (CMS), were prepared by cleaning, ultrasonication and pulverizing processes of biowastes, and then applied to intumescent fire-retardant coatings. The effects of shell bio-fillers with different polymorphs on the fire resistance and char-forming of intumescent fire-retardant coatings were investigated by cone calorimeter test, fire protection tests, smoke density test, thermogravimetric analysis (TG), and the fire resistance and char-forming mechanism of bio-fillers in intumescent fire-retardant coatings were proposed. The results show that three kinds of bio-fillers exert an excellent synergistic effect on enhancing the fire resistance and char-forming properties of the intumescent fire-retardant coatings, while clamshell has the best synergistic efficiency among the bio-fillers. Especially, IFRC-CMS coating containing 3 wt% clamshell shows the best fire protection performance and lowest smoke production and heat release, which offers an equilibrium backside temperature of 134.6 °C at 900 s, a flame-spread rating of 14.4, and a smoke density rating value of 22.8%. The synergistic efficiency of bio-fillers in the intumescent coatings depends on the polymorphs of CaCO3 in bio-fillers, and aragonite CaCO3 shows a higher synergistic efficiency compared to calcite CaCO3 and the mixture of aragonite and calcite CaCO3. The CMS composed of aragonite shows the best synergistic effect, CHS composed of aragonite and calcite comes second, and CES composed of calcite has the weakest synergistic effect.
Material Extrusion-Based Additive Manufacturing of Poly(Lactic Acid) Antibacterial Filaments—A Case Study of Antimicrobial Properties
Authors: Gruber Piotr , Hoppe Viktoria , Grochowska Emilia , Paleczny Justyna , Junka Adam , Smolina Irina , Kurzynowski Tomasz
Published: December 2021

In the era of the coronavirus pandemic, one of the most demanding areas was the supply of healthcare systems in essential Personal Protection Equipment (PPE), including face-shields and hands-free door openers. This need, impossible to fill by traditional manufacturing methods, was met by implementing of such emerging technologies as additive manufacturing (AM/3D printing). In this article, Poly(lactic acid) (PLA) filaments for Fused filament fabrication (FFF) technology in the context of the antibacterial properties of finished products were analyzed. The methodology included 2D radiography and scanning electron microscopy (SEM) analysis to determine the presence of antimicrobial additives in the material and their impact on such hospital pathogens as Staphylococcus aureus, Pseudomonas aeruginosa, and Clostridium difficile. The results show that not all tested materials displayed the expected antimicrobial properties after processing in FFF technology. The results showed that in the case of specific species of bacteria, the FFF samples, produced using the declared antibacterial materials, may even stimulate the microbial growth. The novelty of the results relies on methodological approach exceeding scope of ISO 22196 standard and is based on tests with three different species of bacteria in two types of media simulating common body fluids that can be found on frequently touched, nosocomial surfaces. The data presented in this article is of pivotal meaning taking under consideration the increasing interest in application of such products in the clinical setting.
Value Configurations for Data and Connectivity Solutions in Digitalized Future Factories
Authors: Mansoori Solmaz , Khan Iqra Sadaf , Ahokangas Petri , Matinmikko-Blue Marja , Haapasalo Harri , Yrjölä Seppo
Published: December 2021

The ongoing Industry 4.0 transformation places significant pressures on how businesses create and capture value. Technological advancements such as next-generation mobile communications are reshaping the business ecosystem of Industry 4.0, resulting in emerging business opportunities for new players, such as local operators, to collaborate and compete with mobile communications companies that are implementing I4.0. These changes raise the need to explore emerging business opportunities concerning the digitalization of future factories. New data and connectivity services are introduced to serve the needs of rapidly increasing machine-type communications that rely on connectivity, primarily through the fifth generation (5G) mobile solutions provided by local operators. Thus, this paper outlines the potential value configurations for data and connectivity solutions by identifying, matching, and bridging the utilizable resources and addressable needs within the factory processes. The research applies an exploratory approach and uses the Gioia method to analyze qualitative data of a single case. The study follows the connectivity-content-context-commerce typology (4C) of Internet business models to structure, analyze, and classify the identified needs and resources in future factories from the perspective of the local operator. The findings show that the content layer of 4C business model typology is the most dominant among data and connectivity-based needs and resources of future factories. The paper contributes by presenting four alternative value configurations for digitalization for local operators in the future factory context: the product, component, platform, and complementary businesses. The results suggest content- and context-specific businesses carries foremost business potential for local operators, however quantitative validation will bring fruitful research avenues. Multiple case studies and different data collection methods may also be considered in future studies.
A Comparative Analysis of Vanadyl Porphyrins Isolated from Resins of Heavy Oils with High and Low Vanadium Content
Authors: Yakubov Makhmut , Abilova Guzalia , Tazeeva Elvira , Yakubova Svetlana , Tazeev Damir , Mironov Nikolay , Milordov Dmitriy
Published: December 2021

The composition of purified vanadyl porphyrins recovered from the resins of heavy oils possessing high and low vanadium contents was investigated. Vanadium content in the resins of the heavy oils under study differs by a factor of ca. 15. To recover and purify vanadyl porphyrins from the resins, extraction by N,N–dimethylformamide (DMF) with subsequent two-stage column chromatography on silica gel and sulfocationite were employed. The change of structural-group composition and content of vanadyl porphyrins in the products obtained at each stage was evaluated using Fourier IR and UV-Vis spectroscopy. Analysis of the purified vanadyl porphyrins using MALDI mass spectrometry determined distribution of their most abundant types (etio- and DPEP) and identified C27–C39 homologs for the resins possessing high vanadium content and C28–C39 homologs for the resins with low vanadium content.
Empirical Evidence for the Functionality Hypothesis in Motor Learning: The Effect of an Attentional Focus Is Task Dependent
Authors: Wähnert Svetlana , Müller-Plath Gisela
Published: December 2021

A large body of research suggests that during learning motor skills, focusing on environmental effects of the movement (external focus) generally leads to better performance than focusing on one’s own body (internal focus). The functionality hypothesis states, in contrast, that the superiority of any attentional focus is task dependent. The present study aimed to test the predictions of the latter and searched for underlying mechanisms and task characteristics for one or the other focus being more functional. In Experiment 1, we examined whether the internal focus is superior in a difficult body-oriented balance task. In Experiment 2, we added visual feedback and investigated whether this would enhance the functionality of the external focus. In both experiments, the participants stood one-legged on a balance board and had to shift their centre of pressure (COP) to predefined target points. Per instruction, they were asked to interpret their attentional focus on the COP as either internal (the sole of the foot) or external (the platform). In Experiment 1, the external focus was induced through a mental image. The internal focus group performed significantly better, thereby supporting the functionality hypothesis. In Experiment 2, the COP was dynamically visualized on a screen. The internal focus superiority vanished. We suggest that the internal focus is more functional in motor-learning situations that provide more effect information through body-internal senses than through body-external senses. In these cases, the external focus hampers learning because it is associated with additional cognitive load.
Bioconversions of Biodiesel-Derived Glycerol into Sugar Alcohols by Newly Isolated Wild-Type Yarrowia lipolytica Strains
Authors: Vastaroucha Eleni-Stavroula , Maina Sofia , Michou Savvoula , Kalantzi Ourania , Pateraki Chrysanthi , Koutinas Apostolis A. , Papanikolaou Seraphim
Published: December 2021

The utilization of crude glycerol, generated as a by-product from the biodiesel production process, for the production of high value-added products represents an opportunity to overcome the negative impact of low glycerol prices in the biodiesel industry. In this study, the biochemical behavior of Yarrowia lipolytica strains FMCC Y-74 and FMCC Y-75 was investigated using glycerol as a carbon source. Initially, the effect of pH value (3.0–7.0) was examined to produce polyols, intracellular lipids, and polysaccharides. At low pH values (initial pH 3.0–5.0), significant mannitol production was recorded. The highest mannitol production (19.64 g L−1) was obtained by Y. lipolytica FMCC Y-74 at pH = 3.0. At pH values ranging between 5.0 and 6.0, intracellular polysaccharides synthesis was favored, while polyols production was suppressed. Subsequently, the effect of crude glycerol and its concentration on polyols production was studied. Y. lipolytica FMCC Y-74 showed high tolerance to impurities of crude glycerol. Initial substrate concentrations influence polyols production and distribution with a metabolic shift toward erythritol production being observed when the initial glycerol concentration (Gly0) increased. The highest total polyols production (=56.64 g L−1) was obtained at Gly0 adjusted to ≈120 g L−1. The highest polyols conversion yield (0.59 g g−1) and productivity (4.36 g L−1 d−1) were reached at Gly0 = 80 g L−1. In fed-batch intermittent fermentation with glycerol concentration remaining ≤60 g L−1, the metabolism was shifted toward mannitol biosynthesis, which was the main polyol produced in significant quantities (=36.84 g L−1) with a corresponding conversion yield of 0.51 g g−1.
Non-Destructive Monitoring of Maize Nitrogen Concentration Using a Hyperspectral LiDAR: An Evaluation from Leaf-Level to Plant-Level
Authors: Bi Kaiyi , Niu Zheng , Xiao Shunfu , Bai Jie , Sun Gang , Wang Ji , Han Zeying , Gao Shuai
Published: December 2021

Advanced remote sensing techniques for estimating crop nitrogen (N) are crucial for optimizing N fertilizer management. Hyperspectral LiDAR (HSL) data, with both spectral and spatial information of the targets, can extract more plant properties than traditional LiDAR and hyperspectral imaging systems. In this study, we tested the ability of HSL in terms of estimating maize N concentration at the leaf-level by using spectral indices and partial least squares regression (PLSR) methods. Subsequently, the N estimation was scaled up to the plant-level based on HSL point clouds. Biomass, extracted with structural proxies, was utilized to exhibit its supplemental effect on N concentration. The results show that HSL has the ability to extract N concentrations at both the leaf-level and the canopy-level, and PLSR showed better performance (R2 > 0.6) than the single spectral index (R2 > 0.4). In comparison to the stem height and maximum canopy width, the plant height had the strongest ability (R2 = 0.88) to estimate biomass. Future research should utilize larger datasets to test the viability of using HSL to monitor the N concentration of crops, which is beneficial for precision agriculture.
Utilization of Hyperspectral Remote Sensing Imagery for Improving Burnt Area Mapping Accuracy
Authors: Nolde Michael , Plank Simon , Riedlinger Torsten
Published: December 2021

Wildfires pose a direct threat when occurring close to populated areas. Additionally, their significant carbon and climate feedbacks represent an indirect threat on a global, long-term scale. Monitoring and analyzing wildfires is therefore a crucial task to increase the understanding of interconnections between fire and ecosystems, in order to improve wildfire management activities. This study investigates the suitability of 232 different red/near-infrared band combinations based on hyperspectral imagery of the DESIS sensor with regard to burnt area detection accuracy. It is shown that the selection of wavelengths greatly influences the detection quality, and that especially the utilization of lower near-infrared wavelengths increases the yielded accuracy. For burnt area analysis based on the Normalized Difference Vegetation Index (NDVI), the optimal wavelength range has been found to be 660–670 nm and 810–835 nm for the red band and near-infrared band, respectively.