bluuebunny/crossref_metadata_2025 huggingface dataset:
An efficient protocol for extracting thylakoid membranes and total leaf proteins from Posidonia oceanica and other polyphenol-rich plants
Authors: Charras Quentin , Rey Pascal , Guillemain Dorian , Dourguin Fabian , Laganier Hugo , Peschoux Sacha , Molinié Roland , Ismaël Marwa , Caffarri Stefano , Rayon Catherine , Jungas Colette
Published: March 2024

Background

The extraction of thylakoids is an essential step in studying the structure of photosynthetic complexes and several other aspects of the photosynthetic process in plants. Conventional protocols have been developed for selected land plants grown in controlled conditions. Plants accumulate defensive chemical compounds such as polyphenols to cope with environmental stresses. When the polyphenol levels are high, their oxidation and cross-linking properties prevent thylakoid extraction.

Results

In this study, we developed a method to counteract the hindering effects of polyphenols by modifying the grinding buffer with the addition of both vitamin C (VitC) and polyethylene glycol (PEG4000). This protocol was first applied to the marine plant Posidonia oceanica and then extended to other plants synthesizing substantial amounts of polyphenols, such as Quercus pubescens (oak) and Vitis vinifera (grapevine). Native gel analysis showed that photosynthetic complexes (PSII, PSI, and LHCII) can be extracted from purified membranes and fractionated comparably to those extracted from the model plant Arabidopsis thaliana. Moreover, total protein extraction from frozen P. oceanica leaves was also efficiently carried out using a denaturing buffer containing PEG and VitC.

Conclusions

Our work shows that the use of PEG and VitC significantly improves the isolation of native thylakoids, native photosynthetic complexes, and total proteins from plants containing high amounts of polyphenols and thus enables studies on photosynthesis in various plant species grown in natural conditions.
Oxymatrine protects articular chondrocytes from IL-1β-induced damage through autophagy activation via AKT/mTOR signaling pathway inhibition
Authors: Lu Jinying , Bian Jiang , Wang Yutong , Zhao Yan , Zhao Xinmin , Wang Gao , Yang Jing
Published: March 2024

Background

Osteoarthritis (OA) is a common degenerative joint disease characterized by persistent articular cartilage degeneration and synovitis. Oxymatrine (OMT) is a quinzolazine alkaloid extracted from the traditional Chinese medicine, matrine, and possesses anti-inflammatory properties that may help regulate the pathogenesis of OA; however, its mechanism has not been elucidated. This study aimed to investigate the effects of OMT on interleukin-1β (IL-1β)-induced damage and the potential mechanisms of action.

Methods

Chondrocytes were isolated from Sprague-Dawley rats. Toluidine blue and Collagen II immunofluorescence staining were used to determine the purity of the chondrocytes. Thereafter, the chondrocytes were subjected to IL-1β stimulation, both in the presence and absence of OMT, or the autophagy inhibitor 3-methyladenine (3-MA). Cell viability was assessed using the MTT assay and SYTOX Green staining. Additionally, flow cytometry was used to determine cell apoptosis rate and reactive oxygen species (ROS) levels. The protein levels of AKT, mTOR, LC3, P62, matrix metalloproteinase-13, and collagen II were quantitatively analyzed using western blotting. Immunofluorescence was used to assess LC3 expression.

Results

OMT alleviated IL-1β-induced damage in chondrocytes, by increasing the survival rate, reducing the apoptosis rates of chondrocytes, and preventing the degradation of the cartilage matrix. In addition, OMT decreased the ROS levels and inhibited the AKT/mTOR signaling pathway while promoting autophagy in IL-1β treated chondrocytes. However, the effectiveness of OMT in improving chondrocyte viability under IL-1β treatment was limited when autophagy was inhibited by 3-MA.

Conclusions

OMT decreases oxidative stress and inhibits the AKT/mTOR signaling pathway to enhance autophagy, thus inhibiting IL-1β-induced damage. Therefore, OMT may be a novel and effective therapeutic agent for the clinical treatment of OA.
Prognostic significance of T lymphocyte subgroups (CD4 and CD8) in lung cancer patients after neoadjuvant chemotherapy
Authors: Elicora Aykut , Yaprak Bayrak Busra , Vural Cigdem , Sezer Huseyin Fatih , Uzun Erkal Semra , Metin Elif
Published: March 2024

Objective

The basis for current and future lung cancer immunotherapy depends on our knowledge of molecular mechanisms of interactions between tumor and immune system cells. Interactions that occur between different intratumoral populations of the same cells are important. In our study, we aimed to evaluate relationship between the clinical and prognostic features and T lymphocyte subgroups of patients with lung tumors after neoadjuvant treatment.

Methods

A total of 72 patients were included in our study, including study group, 39 of whom received neoadjuvant chemotherapy. Clinical/radiological/pathological findings of patients and CD4/CD8 staining rates in peritumoral/intratumoral areas were recorded.

Results

Our study revealed significantly lower intratumoral CD4 + T cell density and lower intratumoral CD4/CD8 ratio in primary tumor after neoadjuvant therapy (respectively, 0.012 and 0.016). Considering tumor types, when control-study groups were compared, inflammation was statistically significant only in adenocarcinoma subtype; intratumoral CD4/CD8 ratio was statistically significant only in squamous-cell carcinoma subtype (respectively, p = 0.0008 and p = 0.0139). When CD4 + T lymphocytes and CD8 + T lymphocytes and CD4/CD8 ratio were compared between control and study groups in low-stage patients according to clinical stages, only intratumoral CD4 + T lymphocyte values and intratumoral CD4/CD8 ratio were significant (respectively, p = 0.0291 ve p = 0.0154).

Conclusion

All cell types of innate and adaptive intratumoral immunity can affect lung cancer tissues simultaneously, and these interactions have a very complex structure. Understanding the tumor microenvironment and the different roles of associated cancer immune cells may lead to the discovery of new targets for immunological therapies and increased survival times in lung cancer.
Public attitudes towards COVID-19 vaccine mandates and vaccine certificates in Canada: a time series study
Authors: Zhu David T. , Hawken Steven , Serhan Mohamed , Graves Frank , Smith Jeff , Wilson Kumanan
Published: March 2024

Introduction

Since the beginning of the pandemic, numerous public health measures such as COVID-19 vaccines, vaccine mandates and vaccination certificates have been introduced to mitigate the spread of COVID-19. Public opinion and attitudes towards these measures have fluctuated in response to the dynamic political, social, and cultural landscape of the pandemic.

Methods

We conducted a time-series study consisting of national cross-sectional surveys between November 2021 to March 2022 to evaluate the Canadian public’s attitudes towards COVID-19 vaccine mandates and vaccine certificates.

Results

When examining public sentiment towards COVID-19 vaccine certificates and proof of vaccination measures, there was a shift in responses over time. The proportion of participants “strongly supporting” these measures decreased from 66.0 to 43.1% between W25(Capacity Limits), −W32 (Mask Mandate Removed), whereas “strongly oppose” was the second most common response and rose from 15.9 to 20.6% during this same time period. Concurrently, when examining participants views surrounding mandates, many participants believed that their province was reopening at “about the right pace”, which remained relatively stable over time (33.0–35.4%) between W28 (Emergency Act)–W32 (Mask Mandate Removed).

Conclusion

Our study’s findings on the public’s attitudes towards COVID-19 vaccine mandates and vaccine certificates in Canada may aid to guide and streamline the implementation of future similar public health interventions. Future research should include extended follow-up and a more comprehensive examination of trust in government institutions and polarized perspectives on vaccine mandates.
Optimisation of sugar and solid biofuel co-production from almond tree prunings by acid pretreatment and enzymatic hydrolysis
Authors: Cuevas-Aranda Manuel , Martínez-Cartas Mª Lourdes , Mnasser Fahd , Karim Adnan Asad , Sánchez Sebastián
Published: March 2024

Almond pruning biomass is an important agricultural residue that has been scarcely studied for the co-production of sugars and solid biofuels. In this work, the production of monosaccharides from almond prunings was optimised by a two-step process scheme: pretreatment with dilute sulphuric acid (0.025 M, at 185.9–214.1 ℃ for 0.8–9.2 min) followed by enzyme saccharification of the pretreated cellulose. The application of a response surface methodology enabled the mathematical modelling of the process, establishing pretreatment conditions to maximise both the amount of sugar in the acid prehydrolysate (23.4 kg/100 kg raw material, at 195.7 ℃ for 3.5 min) and the enzymatic digestibility of the pretreated cellulose (45.4%, at 210.0 ℃ for 8.0 min). The highest overall sugar yield (36.8 kg/100 kg raw material, equivalent to 64.3% of all sugars in the feedstock) was obtained with a pretreatment carried out at 197.0 ℃ for 4.0 min. Under these conditions, moreover, the final solids showed better properties for thermochemical utilisation (22.0 MJ/kg heating value, 0.87% ash content, and 72.1 mg/g moisture adsorption capacity) compared to those of the original prunings.

Graphical Abstract
Predictive value of background parenchymal enhancement on breast magnetic resonance imaging for pathological tumor response to neoadjuvant chemotherapy in breast cancers: a systematic review
Authors: Li Xue , Yan Fuhua
Published: March 2024

Objectives

This review aimed to assess the predictive value of background parenchymal enhancement (BPE) on breast magnetic resonance imaging (MRI) as an imaging biomarker for pathologic complete response (pCR) after neoadjuvant chemotherapy (NACT).

Methods

Two reviewers independently performed a systemic literature search using the PubMed, MEDLINE, and Embase databases for studies published up to 11 June 2022. Data from relevant articles were extracted to assess the relationship between BPE and pCR.

Results

This systematic review included 13 studies with extensive heterogeneity in population characteristics, MRI follow-up points, MRI protocol, NACT protocol, pCR definition, and BPE assessment. Baseline BPE levels were not associated with pCR, except in 1 study that reported higher baseline BPE of the younger participants (< 55 years) in the pCR group than the non-pCR group. A total of 5 studies qualitatively assessed BPE levels and indicated a correlation between reduced BPE after NACT and pCR; however, among the studies that quantitatively measured BPE, the same association was observed only in the subgroup analysis of 2 articles that assessed the status of hormone receptor and human epidermal growth factor receptor 2. In addition, the predictive ability of early BPE changes for pCR was reported in several articles and remains controversial.

Conclusions

Changes in BPE may be a promising imaging biomarker for predicting pCR in breast cancer. Because current studies remain insufficient, particularly those that quantitatively measure BPE, prospective and multicenter large-sample studies are needed to confirm this relationship.
The memory ameliorating effects of novel N-benzyl pyridine-2-one derivatives on scopolamine-induced cognitive deficits in mice
Authors: Pant Swati , Gupta Mohan , Anthwal Tulika , Chauhan Monika , Nain Sumitra
Published: March 2024

Background

Alzheimer's disease (AD), the most common form of progressive dementia in the elderly, is a chronic neurological disorder that decreases cognitive ability. Although the underlying cause of AD is yet unknown, oxidative stress and brain acetylcholine shortage are the key pathogenic causes.

Results

The current study shows that these derivatives have the potential to improve memory in mice by inhibiting scopolamine-induced acetylcholinesterase activity, oxidative and nitrosative stress, and improving locomotor activity and muscle grip strength in the rota rod test. When compared to the illness control, the memory-enhancing potential of novel N-benzyl pyridine-2-one derivatives was highly significant (P < 0.0001).

Conclusions

The observed memory ameliorating effect of novel N-benzyl pyridine-2-one makes them as a a good choice for treatment of individuals with cognitive impairment.

Graphical abstract
Impact of ZnO nanopriming on physiological and biochemical traits of wheat (
Triticum aestivum
L.) seedling
Authors: Pandya Priyanka , Kumar Sushil , Patil Ghanshyam , Mankad Monil , Shah Zarna
Published: March 2024

To ascertain the ideal dosage of ZnO NPs (Zinc Oxide Nanoparticles), we conducted an investigation on the priming effects of varying concentrations of ZnO NPs on germination and physio-biochemical parameters of wheat. In this study, ZnO NPs were synthesized and characterized for their physico-chemical properties followed by confirmation of the formation of ZnO NPs. Throughout this study, wheat seeds were subjected to ZnO NPs at various concentrations of 5, 50, 100, 250, and 500 ppm for a period of 4 h via continuous aeration. The primed seeds were sowed in plastic bags, allowed to grow for 21 days, following which comprehensive evaluations of physio-biochemical attributes were conducted. At 250 ppm, an impressive 100% of seeds successfully germinated compared to the control group. The examined physiological factors such as shoot length, root length, and fresh as well as dry weights of leaf and root tissues all exhibited notable increases with the ascending concentrations of ZnO NP up to 250 ppm. However, beyond this threshold, at 500 ppm, these parameters experienced a decline. Inductively coupled plasma atomic absorption spectrophotometer (ICP-AAS) measurements validated the progressive increase in Zinc content in the nanoprimed seedlings, further affirming the dose-dependent trend. Zinc oxide nanoparticles notably improved key biochemical features, including elevated levels of total chlorophyll, malondialdehyde (MDA), total protein, and the accumulation of osmolytes such as proline and glycine-betaine. Additionally, the presence of ZnO NPs led to increased activity of antioxidant enzymes like superoxide dismutase (SOD) and catalase in a dose-dependent mananer. Collectively, the amassed data underscores the efficacy of the 250 ppm ZnO NPs treatment, which emerged as superior in comparison to both the control group and other administered treatments. These findings underscore the potential of ZnO NPs at a concentration of 250 ppm as a valuable seed nanopriming agent, effectively enhanced germination and robust early-stage growth in young plants.
Probing baryogenesis in f(Q) gravity
Authors: Mishra Sai Swagat , Bhat Aaqid , Sahoo P. K.
Published: March 2024

The origin of matter domination in the Universe is one of the most exciting open puzzles in particle physics and cosmology. Despite many theoretical developments, the actual reason behind baryon-antibaryon asymmetry is still unknown. Our aim here is to examine this phenomenon in the framework of modified gravity theories, which have impressively elucidated the contemporary accelerated expansion of the Universe as well as the early phase. Consequently, this letter sets its sights on the task of constraining a specific variant of modified gravity, namely, f(Q) gravity, in conjunction with gravitational baryogenesis. The power law model and recently proposed DGP-like f(Q) models are considered to find the baryon-to-entropy ratio and compare them with the observed value, that is

. Furthermore, we impose constraints on the additional degrees of freedom introduced by this modified theory of gravity.
Hotter deserts and the impending challenges for the spiny-tailed Lizard in India
Authors: Tatu Avichal , Dutta Sutirtha , Thaker Maria
Published: March 2024

Ectotherms are particularly vulnerable to climate change, especially those living in extreme areas, such as deserts, where species are already thermally constrained. Using the vulnerable herbivorous lizard Saara hardwickii as a model system, we used a multi-pronged approach to understand the thermal ecology of a desert agamid and potential impacts of rising temperatures. Our data included field-based measures of operative temperatures, body temperatures, and activity, as well as lab-based measures of thermal limits, preferences, and sprint speed. As expected, the temperature dependence of locomotor performance and foraging activity were different, and in the worst-case global warming scenario (SSP5-8.5), potential sprint speed may decrease by up to 14.5% and foraging activity may decrease by up to 43.5% by 2099. Burrows are essential thermal refuges, and global warming projections suggest that S. hardwickii may be restricted to burrows for up to 9 h per day by 2099, which would greatly limit critical activities, like foraging and seeking mating opportunities. Overall, we show that key information on thermal ecology, including temperature-sensitive behaviours in the wild, is necessary to understand the multiple ways in which increasing temperatures may influence ectothermic vertebrates, especially for species like S. hardwickii that are already vulnerable to environmental change.
Inverted apicobasal polarity in health and disease
Authors: Pasquier Nicolas , Jaulin Fanny , Peglion Florent
Published: March 2024

Apicobasal epithelial polarity controls the functional properties of most organs. Thus, there has been extensive research on the molecular intricacies governing the establishment and maintenance of cell polarity. Whereas loss of apicobasal polarity is a well-documented phenomenon associated with multiple diseases, less is known regarding another type of apicobasal polarity alteration – the inversion of polarity. In this Review, we provide a unifying definition of inverted polarity and discuss multiple scenarios in mammalian systems and human health and disease in which apical and basolateral membrane domains are interchanged. This includes mammalian embryo implantation, monogenic diseases and dissemination of cancer cell clusters. For each example, the functional consequences of polarity inversion are assessed, revealing shared outcomes, including modifications in immune surveillance, altered drug sensitivity and changes in adhesions to neighboring cells. Finally, we highlight the molecular alterations associated with inverted apicobasal polarity and provide a molecular framework to connect these changes with the core cell polarity machinery and to explain roles of polarity inversion in health and disease. Based on the current state of the field, failure to respond to extracellular matrix (ECM) cues, increased cellular contractility and membrane trafficking defects are likely to account for most cases of inverted apicobasal polarity.
Ex-vivo drug screening of surgically resected glioma stem cells to replace murine avatars and provide personalise cancer therapy for glioblastoma patients
Authors: Gagg Hannah , Williams Sophie T. , Conroy Samantha , Myers Katie N. , McGarrity-Cottrell Connor , Jones Callum , Helleday Thomas , Rantala Juha , Rominiyi Ola , Danson Sarah J. , Collis Spencer J. , Wells Greg
Published: March 2024

With diminishing returns and high clinical failure rates from traditional preclinical and animal-based drug discovery strategies, more emphasis is being placed on alternative drug discovery platforms. Ex vivo approaches represent a departure from both more traditional preclinical animal-based models and clinical-based strategies and aim to address intra-tumoural and inter-patient variability at an earlier stage of drug discovery. Additionally, these approaches could also offer precise treatment stratification for patients within a week of tumour resection in order to direct tailored therapy. One tumour group that could significantly benefit from such ex vivo approaches are high-grade gliomas, which exhibit extensive heterogeneity, cellular plasticity and therapy-resistant glioma stem cell (GSC) niches. Historic use of murine-based preclinical models for these tumours has largely failed to generate new therapies, resulting in relatively stagnant and unacceptable survival rates of around 12-15 months post-diagnosis over the last 50 years. The near universal use of DNA damaging chemoradiotherapy after surgical resection within standard-of-care (SoC) therapy regimens provides an opportunity to improve current treatments if we can identify efficient drug combinations in preclinical models that better reflect the complex inter-/intra-tumour heterogeneity, GSC plasticity and inherent DNA damage resistance mechanisms. We have therefore developed and optimised a high-throughput ex vivo drug screening platform; GliExP, which maintains GSC populations using immediately dissociated fresh surgical tissue. As a proof-of-concept for GliExP, we have optimised SoC therapy responses and screened 30+ small molecule therapeutics and preclinical compounds against tumours from 18 different patients, including multi-region spatial heterogeneity sampling from several individual tumours. Our data therefore provides a strong basis to build upon GliExP to incorporate combination-based oncology therapeutics in tandem with SoC therapies as an important preclinical alternative to murine models (reduction and replacement) to triage experimental therapeutics for clinical translation and deliver rapid identification of effective treatment strategies for individual gliomas.
Risk assessment based on fault tree analysis and analytic hierarchy process in rural areas of Thailand
Authors: Joomjee Ratchanee , Raksin Momthicha , Paengprakhon Yanitha , Duangsri Jaruporn , Turnbull Niruwan
Published: March 2024

Introduction The importance of understanding the factors contributing to road accidents at schools cannot be overstated. This study aims to determine the risk of accidents in situations that could lead to accidents near schools. Methods A total of 10 kindergarten to junior high schools were selected for the study. The research used the Haddon Matrix to classify factors at risk of accidents, risk assessment through fault tree analysis, and the analytic hierarchy process (FTA-AHP) techniques. Fourteen sub-criteria were defined for risk identification, risk probability analysis, and risk assessment of the 10 roads. The likelihood of each event was analyzed using the AHP technique for all schools with an expert choice program. RI (random index) was calculated, and CR (consistency ratio) < 0.10 was considered satisfactory. Results The possibility of human accidents ranked highest in three areas: 1) Risk perception in SC 01, 03, and 02, with probabilities of 69.30%, 61.90%, and 57.4%, respectively. 2) The likelihood of accidents from vehicles/equipment, with the highest probabilities in a) Handling (SC01) at 64.70%, b) Braking (SC07) at 61.90%, and c) Lighting (SC03) at 57.80%. 3) The likelihood of accidents from the environment, with the highest probabilities in 1) driving at excessive speeds in areas SC01, 06, 03, and 09, which were 43.60%, 40.90%, and 40.00%, respectively. Conclusions The impacts of all three main factors were as follows: a) humans had the highest impact in the SC01 area (77.90%), b) vehicles/equipment had the highest impact in the SC01 area (75.90%), and c) the highest environmental impact in the SC01 area was 69.90%. The accident risk assessment revealed the highest risk score in three areas: 1) human risk perception, 2) environment with driving at excessive speeds, and 3) vehicle/equipment, including lighting, braking, and handling.
Distributing Zakatu Kasbil 'Amal as an Alternative to Student Funding, Evidence in Indonesia’s Universities
Authors: Marliyah Marliyah , Dharma Budi , Syarbaini Ahmad Muhaisin B.
Published: March 2024

Background The purpose of this paper is to clarify the use of zakat as an alternative funding for students to pay their tuition fees. Apart from that, this paper also explains how zakat collected from muzakki fixed income known as zakatu kasbil 'amal, which is known as income zakat and is still controversial in Indonesia. Method The study uses a systematic literature review with an inductive approach to find out the theoretical basis of the research questions. The analysis uses a qualitative method that explores significant findings from various literature sources. The findings will combine with the exploration results of several universities in Indonesia that use social funds as a tool for helping students. Result The income zakat controversy mediates using the precautionary principle, which ensures that the projected income indeed exceeds the nisab (1 year) of the income. Some Islamic universities in Indonesia carry out this zakat withdrawal and even use an auto debit system. On the other hand, the distribution of zakat to students is also controversial because zakat is part of worship whose distribution is regulated into nine asnaf. Nevertheless, students may be equated with one of the asnaf so that it becomes permissible to be the subject of zakat distribution. Observation findings at Indonesian Islamic universities even show that the results of zakat distribution strongly influence the success of students’ studies, even if only in aid of tuition fees.
Scoping review of international relations theories in health security: A cue for health diplomacy
Authors: Pattanshetty Sanjay , Inamdar Aniruddha , Dsouza Viola Savy , Bhatt Kiran , Jash Amrita , Gudi Nachiket , Brand Helmut
Published: March 2024

Background Health security as a domain has gained tremendous importance in the recent past. Emerging and re-emerging diseases globally, coupled with the derailment of the determinants of health mainly the socio-political environment, has made health security a cross-cutting entity in diverse fields including International Relations (IR). With the ongoing global polycrisis, the health-related issues which were previously sidelined as a concept of less strategic importance in the IR field, are now contributing to the shift of the world order. This has instilled an increased participation of IR scholars in the discussions and debates on health security concerns. The field of IR contains numerous theoretical lenses through which scholars analyze such situations, policies, and systems of the world. Methods In this paper, we use a scoping review method to inspect how IR theories have been applied in analyzing health security concerns. Results We observed that various diverging IR theories have been used to deliberate on states’ actions in tackling the recent pandemic and have also been prescriptive about the changing notions of multilateralism and international governing organizations. Realism, liberalism, and securitization were among the most frequently applied IR theories in the context of health security discussions. Conclusions This work provides an impetus to enhance the interaction among interdisciplinary teams leading to evolving solutions that can address issues of global importance in the contemporary world.
Spot Spine, a freely available ImageJ plugin for 3D detection and morphological analysis of dendritic spines
Authors: Gilles Jean-Francois , Mailly Philippe , Ferreira Tiago , Boudier Thomas , Heck Nicolas
Published: March 2024

Background Dendritic spines are tiny protrusions found along the dendrites of neurons, and their number is a measure of the density of synaptic connections. Altered density and morphology is observed in several pathologies, and spine formation as well as morphological changes correlate with learning and memory. The detection of spines in microscopy images and the analysis of their morphology is therefore a prerequisite for many studies. We have developed a new open-source, freely available, plugin for ImageJ/FIJI, called Spot Spine, that allows detection and morphological measurements of spines in three dimensional images. Method Local maxima are detected in spine heads, and the intensity distribution around the local maximum is computed to perform the segmentation of each spine head. Spine necks are then traced from the spine head to the dendrite. Several parameters can be set to optimize detection and segmentation, and manual correction gives further control over the result of the process. Results The plugin allows the analysis of images of dendrites obtained with various labeling and imaging methods. Quantitative measurements are retrieved including spine head volume and surface, and neck length. Conclusion The plugin and instructions for use are available at https://imagej.net/plugins/spot-spine.
Practical tips to improve bedside teaching using learning theories and clinical reasoning
Authors: Rotthoff Thomas
Published: March 2024

Bedside teaching strengthens the link between theory and practice. The tips given here, which were derived from various learning theories and models, aim to provide structure to bedside teaching and to make this format effective, even though empirical evidence is still missing for this specific setting. These 10 tips may not always be fully implemented in each bedside teaching, but they should be applied selectively for targeted students. In essence, they are more to be understood as a repertoire of effective methods and are intended to expand the literature and framework concepts already available.
EDUCAÇÃO FÍSICA ESCOLAR EM TEMPOS DE PANDEMIA DE COVID-19:
Authors: CARVALHO UALISSON CLEITON DE ARAÚJO , Fernandes Leal Fabiana Soares
Published: March 2024

O presente estudo visou conhecer, por meio de uma revisão sistemática de literatura, o que foi publicado no Brasil acerca dos desafios e possibilidades do uso das Tecnologias Digitais da Informação e Comunicação (TDICs) como principal ferramenta para o desenvolvimento do ensino da educação física escolar durante a pandemia da COVID-19. Foram realizadas buscas de artigos publicados no período de 2020 a 2022, nas plataformas Scielo e Lilacs. Selecionadas as produções, partiu-se para a análise e discussão de 18 artigos. Os resultados mostram que, dentre os desafios encontradas, os mais intensamente citados foram as dificuldades dos professores adaptarem suas metodologias de ensino com o suporte das TDICS, incluindo a forma de avaliar os alunos bem como a necessidade de formação continuada para aprender a utilizar os novos recursos tecnológicos.  Para os alunos, o maior desafio é o acesso aos equipamentos. No que diz respeito as possibilidades foram destacadas a criação de uma conexão entre a escola e o mundo fora dela, aumentando assim a motivação e prazer dos alunos com as aulas, além de ampliar, tanto para professores quanto para alunos, os conhecimentos e uso das TDICS. Conclui-se que o avanço tecnológico é uma realidade que precisa ser integrada, de forma mais efetiva, no cotidiano educacional. É necessário promover a formação de professores e alunos no uso das TDICs a fim de que elas possam de fato, melhorar o ensino.
Make Connections
Authors:   Zeiss Industrial Quality Solutions
Published: March 2024

As electrical connectors grow in complexity, the role of inspection tools like multi-sensor coordinate measuring machines (CMMs) becomes ever more vital, says ZEISS.
Serum proteins differentially expressed in gestational diabetes mellitus assessed using isobaric tag for relative and absolute quantitation proteomics
Authors: Cao Wei-Li , Yu Cui-Ping , Zhang Ling-Li
Published: March 2024

BACKGROUND

As a well-known fact to the public, gestational diabetes mellitus (GDM) could bring serious risks for both pregnant women and infants. During this important investigation into the linkage between GDM patients and their altered expression in the serum, proteomics techniques were deployed to detect the differentially expressed proteins (DEPs) of in the serum of GDM patients to further explore its pathogenesis, and find out possible biomarkers to forecast GDM occurrence.

AIM

To investigation serum proteins differentially expressed in GDM were assessed using isobaric tag for relative and absolute quantitation (iTRAQ) proteomics and bioinformatics analyses.

METHODS

Subjects were divided into GDM and normal control groups according to the IADPSG diagnostic criteria. Serum samples were randomly selected from four cases in each group at 24-28 wk of gestation, and the blood samples were identified by applying iTRAQ technology combined with liquid chromatography-tandem mass spectrometry. Key proteins and signaling pathways associated with GDM were identified by bioinformatics analysis, and the expression of key proteins in serum from 12 wk to 16 wk of gestation was further verified using enzyme-linked immunosorbent assay (ELISA).

RESULTS

Forty-seven proteins were significantly differentially expressed by analyzing the serum samples between the GDM gravidas as well as the healthy ones. Among them, 31 proteins were found to be upregulated notably and the rest 16 proteins were downregulated remarkably. Bioinformatic data report revealed abnormal expression of proteins associated with lipid metabolism, coagulation cascade activation, complement system and inflammatory response in the GDM group. ELISA results showed that the contents of RBP4, as well as ANGPTL8, increased in the serum of GDM gravidas compared with the healthy ones, and this change was found to initiate from 12 wk to 16 wk of gestation.

CONCLUSION

GDM symptoms may involve abnormalities in lipid metabolism, coagulation cascade activation, complement system and inflammatory response. RBP4 and ANGPTL8 are expected to be early predictors of GDM.
Advanced cervix cancer patient with chemotherapy-induced grade IV myelosuppression achieved complete remission with cadonilimab: A case report
Authors: Zhu Rui , Chen Tian-Ze , Sun Meng-Ting , Zhu Chun-Rong
Published: March 2024

BACKGROUND

The prognosis for patients with advanced metastatic cervix cancer (MCC) is poor, and this disease continues to pose a considerable therapeutic challenge. Despite the administration of first-line regimens consisting of cisplatin, paclitaxel, and bevacizumab, survival rates for patients with metastasis remain poor. The emergence of bispecific antibodies (BsAbs) offers a novel treatment option for patients diagnosed with MCC.

CASE SUMMARY

In this report, we present a patient with MCC who was treated with cadonilimab monotherapy at a dose of 6 mg/kg every two weeks after chemotherapy was proven to be intolerable. The patient exhibited a sustained complete response for a duration of 6 months, demonstrating an optimistic outlook.

CONCLUSION

This case illustrates the considerable efficacy of cadonilimab for treating advanced MCC. Therefore, BsAb therapy is a promising strategy for effectively treating patients with advanced MCC and should be considered as an option when patients are intolerant to standard chemotherapy.
Eccrine porocarcinoma in the tempus of an elderly woman: A case report
Authors: Wu Zhi-Wei , Zhu Wen-Jie , Huang Shan , Tan Qi , You Cong , Hu Dian-Gui , Li Long-Nian
Published: March 2024

BACKGROUND

Eccrine porocarcinoma (EPC) is a rare skin tumor that mainly affects the elderly population. Tumors often present with slow growth and a good prognosis. EPCs are usually distinguished from other skin tumors using histopathology and immunohistochemistry. However, surgical management alone may be inadequate if the tumor has metastasized. However, currently, surgical resection is the most commonly used treatment modality.

CASE SUMMARY

A seventy-four-year-old woman presented with a slow-growing nodule in her left temporal area, with no obvious itching or pain, for more than four months. Histopathological examination showed small columnar and short spindle-shaped cells; thus, basal cell carcinoma was suspected. However, immunohistochemical analysis revealed the expression of cytokeratin 5/6, p63 protein, p16 protein, and Ki-67 antigen (40%), and EPC was taken into consideration. The skin biopsy was repeated, and hematoxylin and eosin staining revealed ductal differentiation in some cells. Finally, the patient was diagnosed with EPC, and Mohs micrographic surgery was performed. We adapted follow-up visits in a year and not found any recurrence of nodules.

CONCLUSION

This case report emphasizes the diagnosis and differentiation of EPC.
Infographic: Chongqing technique
Authors: Shen Jie , Wei Zhiyuan , Sun Dong , Wu Hongri , Wang Xiaohua , Wang Shulin , Luo Fei , Xie Zhao
Published: March 2024

Cite this article: Bone Joint Res 2024;13(3):124–126.
The Euler’s harmonic holomorphic regenerative universe
Authors: DUMITRESCU Horia , CARDOS Vladimir , BOGATEANU Radu
Published: March 2024

The Cartesian dualism is a precursor to Euler’s complex theory, that completes the Descartes-Leibnitz monadic conception using the natural quanta (non-splitting e, π) along with their topological torsion in the form of dual isomorphism. The complete Euler’s identity controls a bounded regenerative/ recurrent multiverse (a kind of multigraph) by two regenerative exponential functions, one quantic, e = exp (1) and another gravitational, g0 ≡ 10 = exp (1) with the fixed points, g0 = π2 and (g0g0) respectively. Physically, the fixed points give the well-defined the unit gravity (g0 m/s2) and light self-ignition velocity of a stable recurrent self-sustained process, provided the rate of mass production just equals the rate removal. This is the Euler’s fictitious regenerative universe - like our world, a quantum autocatalytic reaction system. The present paper describes such a system controlled by thermal gravitational waves, in the case the critical solar system.
3D parallel pulsed chaos LiDAR system
Authors: Xiong Wei , Bai Qingsong , Hu Yuqi , Zhang Xing , Wu Yilu , Xia Guangqiong , Zhou Heng , Wu Jiagui , Wu Zhengmao
Published: March 2024

We propose and experimentally demonstrate a parallel pulsed chaos light detection and ranging (LiDAR) system with a high peak power, parallelism, and anti-interference. The system generates chaotic microcombs based on a chip-scale Si3N4 microresonator. After passing through an acousto-optic modulator, the continuous-wave chaotic microcomb can be transformed into a pulsed chaotic microcomb, in which each comb line provides pulsed chaos. Thus, a parallel pulsed chaos signal is generated. Using the parallel pulsed chaos as the transmission signal of LiDAR, we successfully realize a 4-m three-dimensional imaging experiment using a microelectromechanical mirror for laser scanning. The experimental results indicate that the parallel pulsed chaos LiDAR can detect twice as many pixels as direct detection continuous wave parallel chaos LiDAR under a transmission power of -6 dBm, a duty cycle of 25%, and a pulse repetition frequency of 100 kHz. By further increasing the transmission power to 10 dBm, we acquire an 11 cm × 10 cm image of a target scene with a resolution of 30 × 50 pixels. Finally, the anti-jamming ability of the system is evaluated, and the results show that the system can withstand interferences of at least 15 dB.
2 W, 1.5 µm single-mode fiber methane Raman laser pumped by a Yb-doped fiber amplifier
Authors: Pei Wenxi , Lei Luohao , Shi Jing , Li Xuanxi , Huang Wei , Zhou Zhiyue , Li Zhixian , Wang Zefeng
Published: March 2024

We report here, to the best of our knowledge, the first 1.5 µm methane-filled fiber Raman laser pumped by a fiber laser. Based on the narrow-linewidth pulsed Yb-doped fiber laser pump source and a 15 m hollow-core fiber filled with 2.5 bar methane, the maximum power of 2.06 W Stokes wave at 1543 nm is obtained. The output laser has a narrow linewidth of 2.3 GHz, and the pulse repetition frequency can be adjusted flexibly. The output shows excellent near-diffraction-limited beam quality with a M2 factor of ∼1.09. This work proves the advantage of the fiber laser pump source with modest peak power and flexible temporal characteristics in 1.5 µm fiber gas Raman laser emission, providing good guidance for generating pulsed fiber source with narrow linewidth and high beam quality.
High-sensitivity fiber optic graphene resonant accelerometer
Authors: Liu Yujian , Li Cheng , Li Jing , Wan Zhen , Fan Shangchun
Published: March 2024

This study proposes a high-sensitivity resonant graphene accelerometer based on a pressure-induced sensing mechanism. The accelerometer design encompasses an optical fiber and a vacuum-sealed graphene resonator affixed to a silicon sensitive film, incorporating a proof mass. This indirect sensing mechanism effectively mitigates the vibration mode aliasing of graphene and the proof mass while ensuring a minimal energy loss in the operating resonator. The mechanical vibration of graphene is excited and detected through an all-fiber optical system. Notably, the proposed sensor demonstrates a sensitivity of 34.3 kHz/g within the range of 0–3.5 g, which is eight times higher than comparable accelerometers utilizing a proof mass on a graphene membrane. This work exhibits a novel, to the best of our knowledge, approach to an acceleration measurement using 2D resonators, exhibiting distinct advantages in terms of compact size and heightened sensitivity.
High-energy, frequency-doubled thulium-doped fiber chirped-pulse amplification system at 950 nm
Authors: Xu Shutao , Lim Timothy , Turnali Ahmet , Sander Michelle Y.
Published: March 2024

Compact, high-energy ultrafast sources at less exploited fiber gain wavelengths can enable various applications. Here, a high pulse-energy (138 nJ) femtosecond (390 fs) laser source with a center wavelength around 950 nm is demonstrated by frequency-doubling of ultrafast pulses from a thulium-doped fiber chirped-pulse amplification system. The thulium-doped fiber system generates an average output power of 6.5 W around a wavelength of 1.9 µm whose pulses (703 nJ) can be compressed to a pulse duration of 490 fs. This ultrafast 950 nm source offers an attractive system for applications, including two-photon microscopy, and marks the highest-energy fiber system at this wavelength to date.
IntAct: A nondisruptive internal tagging strategy to study the organization and function of actin isoforms
Authors: van Zwam Maxime C. , Dhar Anubhav , Bosman Willem , van Straaten Wendy , Weijers Suzanne , Seta Emiel , Joosten Ben , van Haren Jeffrey , Palani Saravanan , van den Dries Koen
Published: March 2024

Mammals have 6 highly conserved actin isoforms with nonredundant biological functions. The molecular basis of isoform specificity, however, remains elusive due to a lack of tools. Here, we describe the development of IntAct, an internal tagging strategy to study actin isoforms in fixed and living cells. We identified a residue pair in β-actin that permits tag integration and used knock-in cell lines to demonstrate that IntAct β-actin expression and filament incorporation is indistinguishable from wild type. Furthermore, IntAct β-actin remains associated with common actin-binding proteins (ABPs) and can be targeted in living cells. We demonstrate the usability of IntAct for actin isoform investigations by showing that actin isoform-specific distribution is maintained in human cells. Lastly, we observed a variant-dependent incorporation of tagged actin variants into yeast actin patches, cables, and cytokinetic rings demonstrating cross species applicability. Together, our data indicate that IntAct is a versatile tool to study actin isoform localization, dynamics, and molecular interactions.
A single amino acid polymorphism in natural Metchnikowin alleles of Drosophila results in systemic immunity and life history tradeoffs
Authors: Perlmutter Jessamyn I. , Chapman Joanne R. , Wilkinson Mason C. , Nevarez-Saenz Isaac , Unckless Robert L.
Published: March 2024

Antimicrobial peptides (AMPs) are at the interface of interactions between hosts and microbes and are therefore expected to be rapidly evolving in a coevolutionary arms race with pathogens. In contrast, previous work demonstrated that insect AMPs tend to evolve more slowly than the genome average. Metchikowin (Mtk) is a Drosophila AMP that has a single amino acid residue that segregates as either proline (P) or arginine (R) in populations of four different species, some of which diverged more than 10 million years ago. These results suggest that there is a distinct functional importance to each allele. The most likely hypotheses are driven by two main questions: does each allele have a different efficacy against different specific pathogens (specificity hypothesis)? Or, is one allele a more potent antimicrobial, but with a host fitness cost (autoimmune hypothesis)? To assess their functional differences, we created D. melanogaster lines with the P allele, R allele, or Mtk null mutation using CRISPR/Cas9 genome editing and performed a series of life history and infection assays to assess them. In males, testing of systemic immune responses to a repertoire of bacteria and fungi demonstrated that the R allele performs as well or better than the P and null alleles with most infections. Females show some results that contrast with males, with Mtk alleles either not contributing to survival or with the P allele outperforming the R allele. In addition, measurements of life history traits demonstrate that the R allele is more costly in the absence of infection for both sexes. These results are consistent with both the specificity hypothesis (either allele can perform better against certain pathogens depending on context), and the autoimmune hypothesis (the R allele is generally the more potent antimicrobial in males, and carries a fitness cost). These results provide strong in vivo evidence that differential fitness with or without infection and sex-based functional differences in alleles may be adaptive mechanisms of maintaining immune gene polymorphisms in contrast with expectations of rapid evolution. Therefore, a complex interplay of forces including pathogen species and host sex may lead to balancing selection for immune genotypes. Strikingly, this selection may act on even a single amino acid polymorphism in an AMP.
A hybrid feature selection algorithm combining information gain and grouping particle swarm optimization for cancer diagnosis
Authors: Yang Fangyuan , Xu Zhaozhao , Wang Hong , Sun Lisha , Zhai Mengjiao , Zhang Juan
Published: March 2024

Background

Cancer diagnosis based on machine learning has become a popular application direction. Support vector machine (SVM), as a classical machine learning algorithm, has been widely used in cancer diagnosis because of its advantages in high-dimensional and small sample data. However, due to the high-dimensional feature space and high feature redundancy of gene expression data, SVM faces the problem of poor classification effect when dealing with such data.

Methods

Based on this, this paper proposes a hybrid feature selection algorithm combining information gain and grouping particle swarm optimization (IG-GPSO). The algorithm firstly calculates the information gain values of the features and ranks them in descending order according to the value. Then, ranked features are grouped according to the information index, so that the features in the group are close, and the features outside the group are sparse. Finally, grouped features are searched using grouping PSO and evaluated according to in-group and out-group.

Results

Experimental results show that the average accuracy (ACC) of the SVM on the feature subset selected by the IG-GPSO is 98.50%, which is significantly better than the traditional feature selection algorithm. Compared with KNN, the classification effect of the feature subset selected by the IG-GPSO is still optimal. In addition, the results of multiple comparison tests show that the feature selection effect of the IG-GPSO is significantly better than that of traditional feature selection algorithms.

Conclusion

The feature subset selected by IG-GPSO not only has the best classification effect, but also has the least feature scale (FS). More importantly, the IG-GPSO significantly improves the ACC of SVM in cancer diagnostic.