bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Nexus Between Fertility Rates and Geopolitical Risk with Intelligence Methods: A Multifaceted Analysis
Authors: Tzitiridou-Chatzopoulou Maria , Zournatzidou Georgia , Tsakiridis Ioannis , Tsakalidis Christos
Published: November 2024

Background/Objectives: This paper presents an analysis of birth rate statistics, specifically focusing on recorded births in Scotland. The main research objective focuses on investigating the influence of geopolitical concerns on birth rate forecasts. Specifically, we examine whether individuals may choose to postpone or abstain from having children during times of conflict or political turmoil due to concerns about personal safety, the welfare of their children, or uncertainty about the future caused by geopolitical risks. Additionally, this study examines how disruptions to healthcare services, such as limited access to prenatal care and maternal health facilities, can affect birth outcomes and lead to changes in birth rates. Methods: To approach the research objective both machine learning algorithms and classical statistical procedures. Also, as part of the current analysis, the Geopolitical Risk Index has been applied as an extra factor to predict the birth rate. Results: The results of our study demonstrate the effectiveness of machine learning in producing precise predictions in this field, while emphasizing the significant influence of geopolitical risk on comprehending the dynamics of birth rates in Scotland. Conclusions: This study examines the effectiveness of several machine learning regression models in accurately predicting the number of births in Scotland using data that is not included in the model training process. Findings show promising outcomes in predicting births, while geopolitical instability has been indicated as a substantial influence on birth rates and fertility rates.
Double Duty: SGLT2 Inhibitors as Cardioprotective and Anticancer Allies
Authors: Piras Linda , Zuccanti Michela , Tini Melato Giacomo , Volpe Massimo , Tocci Giuliano , Barbato Emanuele , Battistoni Allegra
Published: November 2024

Sodium glucose cotransporter-2 inhibitors (SGLT2i), originally developed for type II diabetes mellitus, have recently been approved for the treatment of heart failure in both diabetic and non-diabetic patients due to their significant cardiovascular benefits. Beyond their established role in diabetes and heart failure management, current research is exploring the potential applications of SGLT2 inhibitors in the field of cardio-oncology. This interest is driven by dual possible benefits: cardioprotection against the adverse effects of antitumor therapies and inherent antitumor properties. Patients affected by cancer often face the challenge of managing cardiovascular toxicity induced by antineoplastic treatments. SGLT2 inhibitors have shown promise in mitigating toxicities, thereby enhancing the cardiovascular health of these patients. Additionally, emerging evidence suggests that SGLT2 inhibitors may possess direct antitumor effects, further contributing to their therapeutic potential in oncology. This review aims to provide a comprehensive overview of the molecular mechanisms through which SGLT2 inhibitors exert their cardioprotective and antitumor effects. Furthermore, we will examine the current body of evidence supporting the use of these inhibitors in a cardio-oncology setting.
Engendering Literary History: Jean-Paul Sartre’s What Is Literature?
Authors: Doran Christine
Published: November 2024

Immediately after the Second World War, Jean-Paul Sartre offered a history of literature as part of his project to launch a new era of literary activity guided by his concept of littérature engagée or committed literature. This article examines Sartre’s approach to the construction of literary history, highlighting his use of periodisation, a thematics of shifting relationships between writers and readers, and frequent deployment of gendered rhetoric to support his arguments. It shows that Sartre repeatedly used gendered tropes that worked to associate women, females and/or femininity with characteristics generally devalued in European and other Western societies, such as passivity, ignorance and indecision. It is argued that the touchstone to which Sartre continually referred in formulating his literary history was Julien Benda’s La Trahison des Clercs (Treason of the Intellectuals). The argument to be developed takes broad inspiration from the work of Hayden White on the analysis of historical texts, and follows his injunction that historians and readers of history need to become more conscious of how histories are made.
What (Counter) Monuments for Feminism? The Debates over Monumental Commemoration and the Search for New Feminist Memory Frameworks
Authors: Sorin Claire
Published: November 2024

At the intersection of memory and feminist studies, this article examines the issue of suffrage and feminist monumental commemoration in the United States. Starting from the deficit of statues representing female historical figures in the public space, it analyzes the conception and reception of two important monuments honoring women’s suffrage (Portrait Monument 1921 and the Women’s Rights Pioneers Monument 2020). While those monuments have somewhat broken the “bronze ceiling”, they testify to the mechanics of exclusion and inclusion at work both in the construction of history and memory. Then, the article takes on a broader perspective, questioning the extent to which traditional monuments, as products of a patriarchal culture and memory, can properly commemorate modern feminism. The essay identifies two trends, one consisting of transforming the bronze through various strategies, the other of “breaking the bronze” by replacing it with other materials and proposing new memory frameworks belonging to what James E. Young has labeled countermonuments. Still, the article ultimately questions the limits of the monument itself and points to the notion of interactive spaces as perhaps the most adequate sites of memory for the complex, multifaceted, contested, and contemporary movement that feminism(s) stand(s) for.
Rooting Conditions for Production of Guarana Clonal Seedlings
Authors: Pinto Karla Gabrielle Dutra , Parédio Rocinádia Franco , Atroch André Luiz , Filho Firmino José do Nascimento , Leite Naara Raana da Costa , Konrad Bruna Nogueira Leite , Albertino Sônia Maria Figueiredo
Published: November 2024

The guaraná tree is a species known for the stimulating properties found in its seeds, leading to increased consumption and promising production. Cultivation is primarily carried out in family farming systems, and one of the challenges in the production process is the acquisition of seedlings. The conventional nursery system, which employs intermittent misting for the production of guaraná clones, raises the cost of seedlings, necessitating the exploration of alternatives to this approach. The aim of this study was to evaluate different conditions as alternatives to the conventional nursery system for rooting guaraná cuttings. The experimental design was entirely randomized, featuring three guaraná genotypes, BRS Maués, BRS CG 611, and BRS Luzeia, alongside two rooting conditions, the conventional nursery and a humid chamber with nebulization, with four repetitions. The rooting of guaraná clones was significantly influenced by the interaction between conditions and genotypes. The humid chamber with nebulization demonstrated superior root system characteristics among the tested conditions, with a rooting rate of 56.66% for BRS Luzeia. This method proved to be efficient and accessible for small producers, making it a satisfactory option for rooting guaraná trees.
Genome-Wide Characterization of Class III Peroxidases and Their Expression Profile During Mycorrhizal Symbiosis and Phosphorus Deprivation in Lettuce (Lactuca sativa L.)
Authors: Simoni Samuel , Castellacci Marco , Usai Gabriele , Rogo Ugo , Mascagni Flavia , Giordani Tommaso , Natali Lucia , Cavallini Andrea , Vangelisti Alberto
Published: November 2024

Lettuce cultivation requires high fertilizer inputs, which impact the environment and costs. Arbuscular mycorrhizal symbiosis (AMS) can reduce fertilizer use, enhance plant nutrition (especially phosphorus), and promote healthier plants. Class III peroxidases (PRXs) play crucial roles in various physiological processes and stress responses. However, their role in AMS and phosphorous (P) deficiency is still unclear. Our study identified 91 PRX genes in the lettuce genome (LsPRXs) and clustered them into eight subfamilies based on phylogenetic relationships. Evolutionary analysis indicated that tandem duplication was the main driver for LsPRX gene family expansion. Synteny analysis showed orthologous relationships of the PRX gene family between lettuce and potato, Arabidopsis, and maize, identifying 39, 28, and 3 shared PRXs, respectively. Transcriptomic data revealed that most LsPRX genes were more expressed in roots than in leaves and differentially expressed LsPRXs were found in response to AMS and P supply. Notably, 15% of LsPRX genes were differentially expressed in roots during mycorrhization. Gene expression network analysis highly correlated five LsPRXs (LsPRX17, LsPRX23, LsPRX24, LsPRX64, and LsPRX79) with genes involved in cell wall remodeling and reorganization during mycorrhization. Our results provide insights into the evolutionary history and functional roles of PRX genes in lettuce and identify candidate gene targets that may enhance the bio-stimulant effects of AMS and help to cope with P deficiency.
Enzyme (α-Glucosidase, α-Amylase, PTP1B & VEGFR-2) Inhibition and Cytotoxicity of Fluorinated Benzenesulfonic Ester Derivatives of the 5-Substituted 2-Hydroxy-3-nitroacetophenones
Authors: Olomola Temitope O. , Nkoana Jackson K. , More Garland K. , Gildenhuys Samantha , Mphahlele Malose J.
Published: November 2024

The prevalence of small multi-target drugs containing a fluorinated aromatic moiety among approved drugs in the market is due to the unique properties of this halogen atom. With the aim to develop potent antidiabetic agents, a series of phenylsulfonic esters based on the conjugation of the 5-substituted 2-hydroxy-3-nitroacetophenones 1a–d with phenylsulfonyl chloride derivatives substituted with a fluorine atom or fluorine-containing (-CF3 or -OCF3) group were prepared. Their structures were characterized using a combination of spectroscopic techniques complemented with a single-crystal X-ray diffraction (XRD) analysis on a representative example. The compounds were, in turn, assayed for inhibitory effect against α-glucosidase, α-amylase, protein tyrosine phosphatase 1 B (PTP1B) and the vascular endothelial growth factor receptor-2 (VEGFR-2) all of which are associated with the pathogenesis and progression of type 2 diabetes mellitus (T2DM). The antigrowth effect of selected compounds was evaluated on the human breast (MCF-7) and lung (A549) cancer cell lines. The compounds were also evaluated for cytotoxicity against the African Green Monkey kidney (Vero) cell line. The results of an in vitro enzymatic study were augmented by molecular docking (in silico) analysis. Their ADME (absorption, distribution, metabolism and excretion) properties have been evaluated on the most active compounds against α-glucosidase and/or α-amylase to predict their drug likeness.
Gelatin-Modified Bioactive Glass for Treatment of Dentin Hypersensitivity
Authors: Tang Mengzhen , Ge Min , Zhang Xu , Zhang Xue’e , Wang Yuxi , Yang Yuhao , Wei Junchao , Yang Jian
Published: November 2024

When dentin is directly exposed to the oral cavity for various reasons, such as a lack of enamel on the tooth surface, external stimuli to the dentin often cause transient discomfort known as dentin hypersensitivity. In order to block the incoming stimulus signal, an ideal treatment is to induce the production of minerals to block the dentinal tubules. In this work, a dentin-desensitizing plugging material was prepared by modifying mesoporous bioactive glass with gelatin, the mineralization and desensitization effects of which were compared with Gluma in in vitro experiments. These experiments confirmed that gelatin-modified bioactive glass (MBG@PDA@Gel) is more effective than traditional desensitizing agents at blocking dentin tubules. Following the successful synthesis of MBG@PDA@Gel, as confirmed by scanning electron microscopy, transmission electron microscopy, and other tests, the treatment of demineralized dentin with MBG@PDA@Gel demonstrated that the dentinal tubules were tightly blocked under scanning electron microscopy. MBG@PDA@Gel induces minerals in deeper layers of dentinal tubules, promoting remineralization and forming a unified structure with the tubule blockage. Animal studies showed that MBG@PDA@Gel can remineralize demineralized dentin, and it is stable in the oral cavity and does not fall out. MBG@PDA@Gel not only enhances the biocompatibility of the nanoparticle but also results in an overall uniform and rapid remineralization of the demineralized dentin.
Single Laboratory Evaluation of the Q20+ Nanopore Sequencing Kit for Bacterial Outbreak Investigations
Authors: Hoffmann Maria , Jang Jay Hee , Tallent Sandra M. , Gonzalez-Escalona Narjol
Published: November 2024

Leafy greens are a significant source of produce-related Shiga toxin-producing Escherichia coli (STEC) outbreaks in the United States, with agricultural water often implicated as a potential source. Current FDA outbreak detection protocols are time-consuming and rely on sequencing methods performed in costly equipment. This study evaluated the potential of Oxford Nanopore Technologies (ONT) with Q20+ chemistry as a cost-effective, rapid, and accurate method for identifying and clustering foodborne pathogens. The study focuses on assessing whether ONT Q20+ technology could facilitate near real-time pathogen identification, including SNP differences, serotypes, and antimicrobial resistance genes. This pilot study evaluated different combinations of two DNA extraction methods (Maxwell RSC Cultured Cell DNA kit and Monarch high molecular weight extraction kits) and two ONT library preparation protocols (ligation and the rapid barcoding sequencing kit) using five well-characterized strains representing diverse foodborne pathogens. High-quality, closed bacterial genomes were obtained from all combinations of extraction and sequencing kits. However, variations in assembly length and genome completeness were observed, indicating the need for further optimization. In silico analyses demonstrated that Q20+ nanopore sequencing chemistry accurately identified species, genotype, and virulence factors, with comparable results to Illumina sequencing. Phylogenomic clustering showed that ONT assemblies clustered with reference genomes, though some indels and SNP differences were observed, likely due to sequencing and analysis methodologies rather than inherent genetic variation. Additionally, the study evaluated the impact of a change in the sampling rates from 4 kHz (260 bases pair second) to 5 kHz (400 bases pair second), finding no significant difference in sequencing accuracy. This evaluation workflow offers a framework for evaluating novel technologies for use in surveillance and foodborne outbreak investigations. Overall, the evaluation demonstrated the potential of ONT Q20+ nanopore sequencing chemistry to assist in identifying the correct strain during outbreak investigations. However, further research, validation studies, and optimization efforts are needed to address the observed limitations and fully realize the technology’s potential for improving public health outcomes and enabling more efficient responses to foodborne disease threats.
The Immunomodulatory Role of Regulatory T Cells in Preterm Birth and Associated Pregnancy Outcomes
Authors: Mureanu Nicoleta , Bowman Amanda M. , Porter-Wright Imogen A. , Verma Priya , Efthymiou Athina , Nicolaides Kypros H. , Scotta Cristiano , Lombardi Giovanna , Tribe Rachel M. , Shangaris Panicos
Published: November 2024

Spontaneous preterm birth (sPTB), defined as live birth before 37 weeks of gestational age, is associated with immune dysregulation and pro-inflammatory conditions that profoundly impact newborn health. The question of immune integrity at the maternal-foetal interface is a focus of recent studies centring not only sPTB but the conditions often affiliated with this outcome. Regulatory T cells (Tregs) play a critical anti-inflammatory role in pregnancy, promoting foetal tolerance and placentation. Due to this gestational role, it is hypothesised that decreased or dysfunctional Tregs may be implicated in cases of sPTB. This review examines studies comparing Treg presence in healthy term pregnancies and those with sPTB-associated conditions. Conflicting findings across different conditions and within sPTB itself have been identified. However, notable findings from the research indicate increased proinflammatory cytokines in pregnancies suffering from premature rupture of membranes (pPROM), chorioamnionitis, infection, preeclampsia, and gestational diabetes (GDM). Additionally, reduced Treg levels were identified in preeclampsia, GDM, and pPROM as well as chorioamnionitis presenting with increased Treg dysfunctionality. Treg deficiencies may contribute to health issues in preterm newborns. Current sPTB treatments are limited, underscoring the potential of in utero therapies targeting inflammation, including T cell interventions. Future research aims to establish consensus on the role of Tregs in sPTB and associated conditions and advancing understanding of mechanisms leading to Treg deficiencies in adverse pregnancy outcomes.
Identification of Malaria-Selective Proteasome β5 Inhibitors Through Pharmacophore Modeling, Molecular Docking, and Molecular Dynamics Simulation
Authors: Yasir Muhammad , Park Jinyoung , Han Eun-Taek , Han Jin-Hee , Park Won Sun , Chun Wanjoo
Published: November 2024

Malaria remains a global health challenge, with increasing resistance to frontline antimalarial treatments such as artemisinin (ART) threatening the efficacy of current therapies. In this study, we investigated the potential of FDA-approved drugs to selectively inhibit the malarial proteasome, a novel target for antimalarial drug development. By leveraging pharmacophore modeling, molecular docking, molecular dynamics (MD) simulations, and binding free-energy calculations, we screened a library of compounds to identify inhibitors selective for the Plasmodium proteasome over the human proteasome. Our results highlighted Argatroban, LM-3632, Atazanavir Sulfate, and Pemetrexed Hydrate as promising candidates, with Argatroban and Pemetrexed Hydrate showing the highest binding affinity and selectivity toward the malarial proteasome. MD simulation and gmx_MMPBSA analysis confirmed the compounds’ ability to remain within the active site of the malarial proteasome, while some exited or exhibited reduced stability within the human proteasome. This study underscores the potential of proteasome-targeting drugs for overcoming malarial drug resistance and paves the way for the further optimization of these compounds.
Factors Affecting the Density of Corneal Endothelial Cells Cultured from Donor Corneas
Authors: Bertolin Marina , Ruzza Alessandro , Barbaro Vanessa , Zanetti Elisa , Ponzin Diego , Ferrari Stefano
Published: November 2024

We investigated which specific correlation exists between the endothelial cell density (ECD) of corneal endothelial cell (CEC) cultures and the features of the donor corneas from which they originate. CEC cultures were prepared from one donor cornea or by pooling together cells of more corneas from elderly donors with ECDs lower or higher than 2000 cells/mm2. The ECDs of such primary cultures were evaluated and showed that that ECDs > 2000 cells/mm2 can be obtained only when CECs are isolated from (1) corneas of young donors; (2) at least two elderly donor corneas (if ECD > 2000 cells/mm2), or three elderly donor corneas (if ECD < 2000 cells/mm2). Secondary cultures are all characterized by ECDs < 2000 cells/mm2. Our study highlights the difficulties in obtaining cultures with ECDs > 2000 cells/mm2. Even if achievable with corneas from young donors, this becomes a challenging task when corneas from elderly donors are used (i.e., the overall majority of those collected by eye banks) and particularly when corneas from elderly donors with ECD < 2000 cells/mm2 are used. Pooling more of two corneas to obtain suitable CECs could technically overcome the problem. The above issues should be tackled appropriately before moving into clinical studies.
Marketable and Banned Pesticides in Agriculture: Categorization, Simulation, and Crystallography Review
Authors: Kyriakopoulos Grigorios L. , Sebos Ioannis
Published: November 2024

Pesticides are playing a dominant role in modern cultivation practices to increase agricultural production but are also criticized for environmental depletion and soil and underground water degradation in field applications. An imperative need for greener pesticides has emerged in alignment with new innovations in agrarian and agricultural practices. This study provides a comprehensive review of marketable and banned pesticides that have been applied in past times or are still in use in agriculture. The collected literature production disclosed 35 distinct pesticides that were identified either isolated or in mixtures and residues. These pesticides are primarily applied in agricultural fields, but some of them were also criticized for human implications. Then, these 35 pesticides were grouped into four categories: insecticides (18), herbicides (9), fungicides (6), and acaricides (2). Furthermore, their molecular types, chemical structures, pKa or log Kow values were presented. Based on their chemical structure, the pesticides were also organized into two domains: “marketable simulated” and “banned simulated”, representing 43% and 57% of total pesticides, respectively. The simulations were generated by linking the elemental composition of each pesticide in the corresponding category; therefore, three “marketable simulated” (the acaricides were not marketable representative) and four “banned simulated” were demonstrated. In addition, the calculation of “adjustment factors” (−0.33 up to +0.50) and the “as calculated/marketable (or banned) simulated pesticides” ratios (0.946 up to 1.013) enabled the identification of four clusters of homogeneous characteristics: cluster 1: “Insecticides, Fungicides, marketable”, cluster 2: “Herbicides, marketable”, cluster 3: “Insecticides, Fungicides, banned”, and cluster 4: “Acaricides, Herbicides, banned”. Subsequently, the composition of the elements of C and H enabled the crystallography characterization of only the “marketable” pesticides, not those that are “banned”, with compounds that have been already registered in the “Crystallography Open Database”. Conclusively, implications, challenges, and future research recommendations have been proposed.
Gut Bacterial Composition and Nutritional Implications in Mexican and Spanish Individuals with Inflammatory Bowel Disease Compared to Healthy Controls
Authors: García-Gamboa Ricardo , Díaz-Torres Osiris , Gradilla-Hernández Misael Sebastián , Pérez-Brocal Vicente , Moya Andrés , González-Avila Marisela
Published: November 2024

The intestinal microbiota plays a key role in the pathogenesis of inflammatory bowel disease (IBD), with its composition varying based on geographic location and dietary factors. This study was performed to examine and compare the bacterial composition of the gut microbiota in Mexican and Spanish individuals with IBD and healthy controls, while also considering the nutritional aspects. This study involved 79 individuals with IBD and healthy controls from Mexico and Spain. The fecal microbiota composition was analyzed using 16S rRNA gene sequencing, and the dietary intake and anthropometric measurements were collected. Alpha diversity analysis revealed a lower Chao1 index of the bacterial genera in the IBD groups. Beta diversity analysis showed significant differences in the bacterial composition, suggesting inter-individual variability within the healthy and IBD groups. Additionally, the relative abundance of the bacterial genera varied across the four groups. Faecalibacterium was more abundant in the IBD groups; Prevotella was found exclusively in the Mexican groups, and Akkermansia was found only in the Spanish groups. Akkermansia was positively correlated with meat and protein intake, Prevotella with lean mass, and Bacteroides with calorie intake. These findings highlight the importance of considering geographic and nutritional factors in future research on the gut microbiome’s role in IBD pathogenesis.
Application of Fetal Membranes and Natural Materials for Wound and Tissue Repair
Authors: Rouzaire Marion , Blanchon Loïc , Sapin Vincent , Gallot Denis
Published: November 2024

The human fetal membrane is a globally accepted biological biomaterial for wound and tissue repair and regeneration in numerous fields, including dermatology, ophthalmology, and more recently orthopedics, maxillofacial and oral surgery, and nerve regeneration. Both cells and matrix components of amnion and chorion are beneficial, releasing a diverse range of growth factors, cytokines, peptides, and soluble extracellular matrix components. Beside fetal membranes, numerous natural materials have also been reported to promote wound healing. The biological properties of these materials may potentiate the pro-healing action of fetal membranes. Comparison of such materials with fetal membranes has been scant, and their combined use with fetal membranes has been underexplored. This review presents an up-to-date overview of (i) clinical applications of human fetal membranes in wound healing and tissue regeneration; (ii) studies comparing human fetal membranes with natural materials for promoting wound healing; and (iii) the literature on the combined use of fetal membranes and natural pro-healing materials.
Comparison Between Electroporation at Different Voltage Levels and Microinjection to Generate Porcine Embryos with Multiple Xenoantigen Knock-Outs
Authors: Fernández Juan Pablo , Petersen Björn , Hassel Petra , Lucas Hahn Andrea , Kielau Paul , Geibel Johannes , Kues Wilfried A.
Published: November 2024

In the context of xenotransplantation, the production of genetically modified pigs is essential. For several years, knock-out pigs were generated through somatic cell nuclear transfer employing donor cells with the desired genetic modifications, which resulted in a lengthy and cumbersome procedure. The CRISPR/Cas9 system enables direct targeting of specific genes in zygotes directly through microinjection or electroporation. However, these techniques require improvement to minimize mosaicism and low mutation rates without compromising embryo survival. This study aimed to determine the gene editing potential of these two techniques to deliver multiplexed ribonucleotide proteins (RNPs) to generate triple-knock-out porcine embryos with a multi-transgenic background. We designed RNP complexes targeting the major porcine xenoantigens GGTA1, CMAH, and B4GALNT2. We then compared the development of mosaicism and gene editing efficiencies between electroporation and microinjection. Our results indicated a significant effect of voltage increase on molecule intake in electroporated embryos, without it notably affecting the blastocyst formation rate. Our gene editing analysis revealed differences among delivery approaches and gene loci. Notably, employing electroporation at 35 V yielded the highest frequency of biallelic disruptions. However, mosaicism was the predominant genetic variant in all RNP delivery methods, underscoring the need for further research to optimize multiplex genome editing in porcine zygotes.
Transcriptome-Based Spatiotemporal Analysis of Drought Response Mechanisms in Two Distinct Peanut Cultivars
Authors: Sun Zexin , Liu Wei , Wang Xinning , Ai Xin , Li Zhao , Zhou Dongying , Ma Qianchi , Li Yujiao , Wang Jiaqi , Ma Xinlei , Wang Xiaoguang , Zhong Chao , Jiang Chunji , Zhao Shuli , Zhang He , Zhao Xinhua , Kang Shuli , Wang Jing , Yu Haiqiu
Published: November 2024

Drought tolerance varies among different peanut (Arachis hypogaea L.) cultivars. Here, drought responses of two cultivars, Huayu 22 (HY22) with drought tolerance and Fuhua 18 (FH18) with drought sensitivity, were compared at the morphological, physiological, biochemical, photosynthetic, and transcriptional levels. Drought stress caused wilting and curling of leaves, bending of stems, and water loss in both cultivars. There was an increase in malondialdehyde (MDA) content under prolonged drought stress, more so in FH18. But the levels of reactive oxygen species (H2O2) and lipid peroxidation were low in HY22. The activities of superoxide dismutase (SOD), peroxidase (POD), and glutathione reductase (GR) were considerably elevated, corresponding with rapid increases in the accumulation of soluble proteins, soluble sugars, and proline. Transcriptional sequencing showed gene expression varied seriously in HY22, which was upregulated in both stems of two cultivars, though downregulation was less pronounced in HY22. KEGG pathway analysis revealed significant enrichment in four leaf and six stem pathways. Additionally, core genes relating to photosynthesis, carbon fixation, proline synthesis, and sucrose and starch synthesis pathways were identified by correlation analysis. Those gene expressions were variously upregulated in stems of two cultivars, especially in HY22, giving a novel view of the shoot as a whole participating in stress response.
Genome-Wide Analysis of HECT E3 Ligases Members in Phyllostachys edulis Provides Insights into the Role of PeHECT1 in Plant Abiotic Stress Response
Authors: Xie Xinru , Hu Songping , Liu Linxiu , Pan Huanhuan , Huang Hu , Cao Xun , Qiao Guirong , Han Xiaojiao , Qiu Wenmin , Lu Zhuchou , Zhuo Renying , Xu Jing
Published: November 2024

Homology to E6-AP Carboxy Terminus (HECT) E3 ubiquitin ligases play pivotal roles in plant growth, development, and responses to abiotic stresses. However, the function of HECT genes in Phyllostachys edulis (P. edulis) remains largely uninvestigated. In this study, a comprehensive genome-wide analysis of the HECT E3 ubiquitin ligases gene family in P. edulis was conducted, aiming to elucidate its evolutionary relationships and gene expansion. Analysis of gene structure, conserved motifs and domains, and synteny genome regions were performed. Furthermore, cis-elements in HECT gene promoters that respond to plant hormones and environmental stresses were identified and corroborated by expression data from diverse abiotic stress conditions and hormone treatments. Based on the co-expression network of PeHECTs under cold and dehydration stresses, PeHECT1 was identified as a key candidate gene associated with abiotic stress tolerance. Overexpression of PeHECT1 in tobacco leaves significantly upregulated genes related to reactive oxygen species (ROS) detoxification and polyamine biosynthesis. Yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and dual-luciferase (dual-LUC) assays suggested that the transcription factor ETHYLENE RESPONSE FACTOR 3 (PeERF3) bound to the dehydration-responsive element (DRE) of the promoter of PeHECT1 and activated its transcription activity. Phylogenetic analysis indicated that PeHECT1 in P. edulis exhibited a close association with the diploid herbaceous bamboo Olyra latifolia, followed by the divergence of rice and bamboo. In summary, this study enhances our comprehensive understanding of the HECT E3 ubiquitin ligases gene family in P. edulis and highlights the potential role of PeHECT1 in plant abiotic stress response.
Genetic Variation Study of Several Romanian Pepper (Capsicum annuum L.) Varieties Revealed by Molecular Markers and Whole Genome Resequencing
Authors: Udriște Anca Amalia , Iordăchescu Mihaela , Bădulescu Liliana
Published: November 2024

Numerous varieties of Capsicum annuum L. with multiple valuable traits, such as adaptation to biotic and abiotic stress factors, can be found in south-east Romania, well known for vegetable cultivation and an important area of biodiversity conservation. To obtain useful information about sustainable agriculture, management, and conservation of local pepper varieties, we analyzed the genetic diversity and conducted deep molecular characterization using whole genome resequencing (WGS) for variant/mutation detection. The pepper varieties used in the present study were registered by VRDS in the ISTIS catalog between 1974 and 2019 and maintained in conservative selection; however, no studies have been published yet using WGS analysis in order to characterize this specific germplasm. The genome sequences, annotation, and alignments provided in this study offer essential resources for genomic research as well as for future breeding efforts using the C. annuum local varieties.
Growth Promotion and Economic Benefits of the Probiotic Lactiplantibacillus plantarum in Calves
Authors: Osawa Kazumasa , Taharaguti Saya , Ito Chiaki , Takino Tadashi , Hagiwara Katsuro
Published: November 2024

Objectives: Various measures have been attempted to prevent infectious diseases in calves, such as environmental improvement and vaccine administration. Probiotics are commonly used to improve the body condition of newborn calves and prevent disease. In our previous research, Lactiplantibacillus plantarum RGU-LP1 (LP1) suppressed the expression of inflammatory cytokines in PBMCs of cattle fed it in the diet. In this study, we evaluated the effect of LP1 on the weights and number of treatments of the calves. Methods: Twenty-six one-week-old Holstein bull calves were divided into two groups (thirteen each), the LP1 group (LP1-treated) and the CN group (no LP1 fed), and tested as follows. The LP1 group was fed lyophilized LP1 (109 CFU/head/day) in milk replacer for 40 days. The CN group was fed the same diet only. Calves were followed for 63 days. The average treatment costs for the LP1 during the period were recorded. Feces and blood were collected from each calf during this period. Feces were examined for gut microbiota, and blood for immune assay and cytokine gene expression. Results: The LP1-treated group showed a decrease in disease incidence and an increase in body weights compared to controls. The average treatment cost during the observation period was significantly reduced compared to the CN group. The expression of TGFβ and IL10, inhibitory cytokines of inflammation, was significantly increased. The simultaneous expression of this set of inhibitory molecules resulted in low serum IL1β levels during the growth period. Conclusions: The Th1-type cytokine IFNγ was also significantly increased in LP1-treated calves. By reducing the amount of disease treatments and increasing dairy gain, LP1 is effective in preventing infectious diseases in calves. In addition, the increase in IFNγ by LP1 indicates improved Th1-type immunity in calves. These results show that LP1 has effects on the regulated inflammatory response and growth of calves.
An Exploratory Study of Small-Group Learning Interactions in Pre-Clerkship Medical Education: Uncovering a Mismatch Between Student Perceptions and Real-Time Observations
Authors: Walton Jennifer M. , Oswald Anna E.
Published: November 2024

Small-group learning is a mainstay of medical education, and group functioning can have a major influence on these learning experiences. Our objective was to explore verbal exchange patterns within small-group learning sessions and examine how different patterns related to tutor involvement, tutor expertise, and participants’ perceptions. A non-participant observer collected group interactivity data using a real-time mobile device-based system. Verbal interaction patterns were visualized and analyzed using social network analysis and correlated with participant survey data and aggregate course grades. There were 46 observations across 30 separate groups. Group interactions clustered into four patterns defined by (1) tutor involvement (high vs. low) and (2) interactivity (high vs. low). Interaction patterns were largely stable for a given group and groups with content expert facilitators were generally less interactive. Students reported objectively fewer interactive groups as more interactive and enjoyable. There were no significant intergroup differences in aggregate course grades. Paradoxically, student perceptions were not aligned with observed interactivity data, and tutor content expertise influenced group interactivity. These findings suggest the need to better manage learner expectations of small-group learning, and to explicitly reflect on and develop skills for effective collaborative learning with both faculty and students.
Is the Taiwan Stock Market (Swarm) Intelligent?
Authors: Chen Ren-Raw
Published: November 2024

It is well-believed that most trading activities tend to herd. Herding is an important topic in finance. It implies a violation of efficient markets and hence, suggests possibly predictable trading profits. However, it is hard to test such a hypothesis using aggregated data (as in the literature). In this paper, we obtain a proprietary data set that contains detailed trading information, and as a result, for the first time it allows us to validate this hypothesis. The data set contains all trades transacted in 2019 by all the brokers/dealers across all locations in Taiwan of all the equities (stocks, warrants, and ETFs). Given such data, in this paper, we use swarm intelligence to identify such herding behavior. In particular, we use two versions of swarm intelligence—Boids and PSO (particle swarm optimization)—to study the herding behavior. Our results indicate weak swarm among brokers/dealers.
The Cap and the Spermatostyle Protecting the Sperm Bundle Have a Similar Origin—Ultrastructural Study of the Spermatogenesis from the Ground Beetle Carabus (Chaetocarabus) lefebvrei Dejean, 1826 (Adephaga Carabidae)
Authors: Lupetti Pietro , Mercati David , Giglio Anita , Brandmayr Pietro , Dallai Romano
Published: November 2024

The males of Carabus lefebvrei produce sperm bundles of quite variable size as a consequence of a sexual selection via sperm competition. The ultrastructural features of spermatogenesis of the species and the sequence of events leading to sperm bundle formation along the deferent duct lumen revealed the origin and evolution of the apical structure protecting the sperm bundles. Elongated layers of the electron-dense material of variable size and thickness are visible over the microvilli of the distal deferent duct epithelium. They likely correspond to sections through different levels of discoidal secretions. Then, the sperm present in the duct lumen attach to one side of such elongated secreted structures. Later on, these structures bent to take a cap shape and increase their size and thickness by apposition of additional secretions produced by the proximal deferent duct epithelium. These results reconcile the different opinions about the formation of the structures called the cap and the rod (spermatostyle). In our work, we in fact obtained evidence that both these structures have the same origin, even though at the end of their formation they exhibit a different appearance. The reported results indicate that, differently from the spermatostyle, in the initial stages of cap formation, the sperm adhere only to one side of the cap rather than around the whole structure, as it occurs instead along the spermatostyle rod. Under this aspect, the cap structure is similar to the protection present in the sperm of some whirligig beetle.
Identification and Functional Analysis of Three NlCstF Genes in Nilaparvata lugens
Authors: Jing Shengli , Wang Feifei , Ren Aobo , Zheng Fang , Yu Bingbing , Xu Jingang , Liu Yali , Yang Jing , Chen Ruixian , Zeng Wei , Zhang Yimei , Ke Danxia , Ma Xiantao , Tang Hengmin , Liu Qingsong , Yu Bin
Published: November 2024

The Cleavage Stimulation Factor (CstF) complex, consisting of three subunits, is essential for the 3′ end processing of precursor messenger RNA (pre-mRNA). In mammals, this complex includes CstF50, CstF64, and CstF77, named according to their molecular weights, and these proteins are conserved across many organisms. However, the functional roles of the three CstF genes (NlCstF50, NlCstF64, and NlCstF77) in Nilaparvata lugens, a major rice pest, have not been fully explored. This study identified and characterized the sequences of these genes, with proteins encoded by NlCstF50, NlCstF64, and NlCstF77 consisting of 439, 419, and 732 amino acids, respectively. These proteins are conserved among various insect species. Spatio-temporal expression analysis revealed that these genes are expressed at all developmental stages and in various tissues, with peak levels in eggs and testes. RNA interference (RNAi) targeting one or all three NlCstF genes resulted in a reduction in gene expression by 68% to 90% at 72 h post-injection, indicating that multi-gene dsRNA can achieve similar silencing outcomes as single-gene dsRNA. Knocking down one or all three NlCstF genes caused significant lethal phenotypes and molting disruptions. Mortality rates increased from 62.5% (dsNlCstF50) to 95.4% (dsNlCstF(50+64+77)). Additionally, silencing these genes reduced the number of eggs laid per female and hatch rates. These results highlight the critical role of NlCstF genes in the development and reproduction of N. lugens, suggesting their potential as targets for RNAi-based pest control strategies.
Shedding Light on the Shadows: Hidden Diversity in the Italian Embioptera
Authors: Cianferoni Fabio , Ceccolini Filippo
Published: November 2024

In order to better understand the differences at the species level in webspinners (Embioptera), the authors applied a different approach rather than the usual slide mounting, which has been traditionally used in the study of these insects. The dissection of the male terminalia, besides avoiding the limits of definitive preparations, which prevent a complete comparison of the type material, allowed a complete understanding and comparison of these three-dimensional structures. This new method, which does not necessarily exclude the previous approach, allowed the authors to identify and describe three new species in Italy: from Tuscany (Embia specolensis sp. nov.), Ventotene Island (Embia pandateriensis sp. nov.), and Calabria (Embia brutia sp. nov.). Moreover, this charts a new path in the study of these interesting insects, which have so far been largely neglected. In addition to several new records resulting from the revision of some collections, this contribution provides an updated checklist and a provisional key for the Italian Embioptera.
Using Egg Parasitoids to Manage Caterpillars in Soybean and Maize: Benefits, Challenges, and Major Recommendations
Authors: Bueno Adeney de F. , Sutil Weidson P. , Cingolani M. Fernanda , Colmenarez Yelitza C.
Published: November 2024

The use of egg parasitoids in Augmentative Biological Control (ABC) is a highly effective strategy within the integrated pest management (IPM) of lepidopteran defoliators. Safer than chemical insecticides, these natural antagonists have demonstrated significant efficacy. Trichogramma pretiosum and Telenomus remus, known for their high parasitism rates, are the most extensively used and studied parasitoids for controlling economically important lepidopterous in crops such as soybean and maize. Brazil, a leading adopter of crops expressing Bacillus thuringiensis (Bt) proteins, faces growing field-evolved resistance to Cry proteins in soybean and maize. This resistance, particularly of Rachiplusia nu in soybean and Spodoptera frugiperda in maize, has become more prominent in recent years, increasing insecticide use. Therefore, this article reviews the current status of egg parasitoids adoption in ABC against lepidopteran pests, emphasizing the role of Tr. pretiosum and the potential of Te. remus as sustainable alternatives to chemical insecticides to manage pests in both non-Bt and Bt crops. Additionally, we provide recommendations for using these parasitoids in ABC programs and discuss the challenges that must be addressed to optimize the adoption of biocontrol agents in ABC programs for maximum benefit.
Pulsed Field Ablation in Atrial Fibrillation: Initial Experience of the Efficacy and Safety in Pulmonary Vein Isolation and Beyond
Authors: Tay Julian Cheong Kiat , Tarranza Jannah Lee , Chia Shaw Yang , Pung Xuan Ming , Loo Germaine Jie Min , Teo Hooi Khee , Yeo Colin , Tan Vern Hsen , Lim Eric Tien Siang , Chong Daniel Thuan Tee , Ho Kah Leng , Ching Chi Keong
Published: November 2024

Regional differences in pulsed field ablation (PFA) adoption for pulmonary vein isolation (PVI) with additional posterior wall ablation (PWA) in Asia remains unknown. We hereby report our experience on the safety and efficacy of PFA in AF ablation. Consecutive AF patients who underwent PFA from September 2022 to January 2024 were included. The primary efficacy endpoint was freedom from atrial arrhythmia recurrence after a 90-day blanking period at 12 months. Safety endpoints included 30 days of all-cause death, cardiac tamponade, stroke, myocardial infarction, and heart failure hospitalization. One hundred and one (72.3% males, 79.2% pAF) patients underwent PFA for AF. Thirty-one (30.7%) had structural heart disease with mean LVEF of 57.4 ± 8.1% and CHA2DS2-VASc score of 1.4 ± 1.3. Twenty-nine (28.7%) underwent additional PWA (PVI + PWA) using PFA. PWA was acutely successful in all patients. Patients who underwent PWA were more likely to have persistent AF and require general anesthesia and electroanatomic mapping (all p < 0.05). Total PFA applications for PVI, LA dwell time, procedural time, and fluoroscopy time were similar between the PVI-only and PVI + PWA groups (all p > 0.05). The 1-year atrial arrhythmia recurrence rates were 10% for pAF and 21% for the persistent AF group. The primary efficacy endpoint was not significantly different between the PVI-only and PVI+PWA groups (12-month KM estimates 90.3% [95% CI, 83.3–97.3] and 82.8% [95% CI, 68.1–97.4], respectively). There were no complications related to PFA use. PFA can be safely, effectively, and efficiently adopted for AF ablation. Additional PWA, if pursued, had similar procedural metrics to the PVI-only strategy without increased complications.
Over-the-Wire Retrieval of Infectious Hemodialysis Catheter-Related Right Atrial Thrombus Causing Recurrent Pleural Empyema and Sepsis: A Case-Based Review
Authors: Barilaro Giuseppe , Galassi Amedeo , Gatto Maria Chiara , Ciocci Giulia , Fabrizio Fabrizia Paola , Cappelli Alessandra
Published: November 2024

Introduction: Infectious catheter-related right atrial thrombus (CRAT) is a potentially fatal but often underestimated contingency associated with central venous catheter (CVC) in patients on hemodialysis. Management guidelines for CRAT are lacking, and its occurrence poses clinical challenges. Here, we describe the case of an infectious CRAT in a young patient on hemodialysis with peculiar clinical complications and perform a literature review. Case presentation and literature review: A 30-year-old man on hemodialysis after bilateral nephrectomy due to polycystic disease presented with hyperpyrexia resistant to broad-spectrum antibiotics. A pleural empyema caused by methicillin-resistant Staphylococcus aureus (MRSA) was diagnosed. Since fever persisted despite targeted antibiotic therapy, a transthoracic echocardiogram to exclude infective endocarditis was performed, showing a right atrial thrombus. CVC was promptly removed and the thrombus was aspirated through a percutaneous retrieval system. The thrombus cultural exam resulted positive for MRSA. After performing an extensive literature review, we could not find another case reporting the concomitance of these two rare complications. Conclusions: CRAT is a life-threatening complication in hemodialysis patients. While various treatment options exist, evidence-based guidelines are lacking, leading to individualized management strategies. Minimizing CVC use remains the best option for preventing such a complication.