bluuebunny/crossref_metadata_2025 huggingface dataset:
The Protective Effects of Silver Fluoride Solution and Fluoride Varnish on Dental Erosion—An In Vivo Study
Authors: Haabeth Brox Julie Marie , Tulek Amela , Sehic Amer , Mulic Aida , Utheim Tor Paaske , Khan Qalbi
Published: January 2025

Objectives: Dental erosion has evolved into a common condition with growing concern in the dental community. The aim of this study was to explore the protective effects of two highly fluoridated products, silver fluoride solution (silver diamine fluoride-potassium iodide solution, SDF-KI) and fluoride varnish, on dental erosion in mouse. Methods: Two groups of ten young CD-1 mouse were given a cola drink ad libitum over a 6-week period. A prophylactic treatment with a silver fluoride solution (38% SDF 48,000 ppm F with a silver concentration of 253,900 ppm) and a fluoride varnish (NaF, 22,600 ppm F) were applied on the mandibular molars, performed under sedation twice a week during the whole experiment. Furthermore, two control groups of ten mice were included, a positive (cola drink) and a negative (distilled water) control. A terminal procedure was followed by dissection of mandibular molars and analysis of them using scanning electron microscopy (SEM). The first molars were transversely ground, further analyzed by SEM, and measured for tooth height and tooth tissue loss. Results: Analyses of mandibular molars treated with a fluoride varnish indicated a 5% decreased tooth tissue loss, compared to the molars which served as a positive control. The best effect was achieved with the application of silver fluoride solution, displaying a 35% decreased tooth tissue loss compared to the positive control group. Conclusions: Preventive treatment with a solution of silver fluoride solution exhibits greater protection against dental erosion compared to a traditional fluoride varnish. This study indicates that silver fluoride solution is an effective fluoride compound and is highly beneficial in a clinical setting with the aim of preventing dental erosion.
Deterministic Quantum Dense Coding Based on Non-Maximal Entangled Channel
Authors: Xin Xuanxuan , Li Zhixing , Wang Zhen
Published: January 2025

In quantum communication, the concept of dense coding traditionally relies on maximally entangled states as quantum channels. Recent advancements have expanded this framework to include non-maximally entangled states within the probabilistic dense coding paradigm. However, such schemes introduce a significant limitation: the receiver cannot always retrieve the complete dense coding information sent by the sender. Consequently, the receiver must inform the sender of the amount of information successfully received. Based on this feedback, the sender determines whether retransmission is necessary, leading to inefficient use of the quantum channel and reduced communication efficiency. To address these shortcomings, we propose an alternative deterministic quantum dense coding scheme that utilizes non-maximally entangled states as the quantum channel. This deterministic approach eliminates the need for retransmissions and significantly enhances communication efficiency while maintaining compatibility with non-maximally entangled states. Our scheme represents a substantial improvement over existing probabilistic methods and paves the way for more efficient quantum communication protocols.
Control Strategy for Asymmetric Faults on the Low-Frequency Side of a Sparse Modular Multilevel Converter
Authors: Sun Yuwei , Wang Shengce , Fu Chao , Zhang Zelin , Zhao Guoliang , Xu Yunfei , Liu Bao , Jia Chen
Published: January 2025

Sparse modular multilevel converters (SMMCs) are a new type of lightweight high-voltage large-power AC/AC converter that significantly reduces the number of components compared to modular multilevel matrix converters (M3Cs). This study proposes a fault ride through a control strategy for SMMC to address the issues of arm energy imbalances and valve-side overvoltage, which occur during asymmetric faults on the low-frequency side. First, we establish models of the energy deviation of the arms under asymmetric short-circuit faults on the low-frequency side of SMMC. We also study the influence mechanism of the control strategies on the arm energy imbalance during faults. On this basis, an arm energy balancing strategy based on zero-sequence voltage injections combined with AC voltage control is proposed; this can achieve arm energy balance and suppress the negative sequence current and overvoltage of the SMMC. Finally, we construct a simulation model of an offshore wind power low-frequency transmission system based on the SMMC. The simulation results show that the proposed energy balance strategy can realize the stable operation of the low-frequency transmission system (LFTS) under asymmetric faults on the low-frequency side, that the maximum capacitor voltage deviation during the fault does not exceed 10% and that capacitor voltage returns to normal 0.25 s after the fault occurs.
Quantifying Socio-Regional Variability via Factor Analysis over China: Optimizing Residential Sector Emission Reduction Pathways
Authors: Zhao Yu , Divigalpitiya Prasanna
Published: January 2025

Policy synergy, the evidence-based coordination of public policies, can aid in more rapidly achieving air pollutant and carbon dioxide (CO2) emission reduction targets. Using logarithmic mean Divisia index (LMDI) decomposition, coupling coordination degree (CCD), and geographically and temporally weighted regression (GTWR) models, we analyzed the emission characteristics, drivers, and reduction pathways of residential air pollution across 30 Chinese provinces from 2001 to 2020. The southern provinces produced more air pollution than the northern provinces, with the gap widening after 2015. In the residential sector, energy emission factors (LMDI decomposition result, 686,681.9) and population size (14,331) had greater impacts on air pollutant emissions than the energy structure, energy intensity, synergies, or GDP per capita. The GTWR analysis of the CCD mechanism indicated that hydroelectricity and urbanization enhanced coupling coordination in the southeast. Meanwhile, in the west, coupling coordination was improved by R&D investment, government spending on industrial pollution control, electricity consumption, per capita cropland, temperature, and urbanization. This analysis provides a valuable reference for optimizing emission reduction strategies.
Spatiotemporal Dynamics of Fine Root Biomass in Chinese Fir (Cunninghamia lanceolata) Stumps and Their Impacts on Soil Chemical Properties
Authors: Bao Yinmanda , Wang Xu , Guo Hao , Zhou Guangyi , Chen Yuehua
Published: January 2025

Stumps are residuals from artificial forest harvesting, persist in forest ecosystems, and have garnered attention for their ecological roles in soil and water conservation, carbon sequestration, and forest regeneration. However, the spatiotemporal dynamics of stump fine root biomass and their impact on soil nutrient cycling remain unclear. This study focuses on the fine roots of Chinese Fir (Cunninghamia lanceolata) stumps generated during the construction of national reserve forests at Xishan State Forest Farm, Linwu County, Hunan Province, from 2014 to 2022. Employing a space-for-time substitution approach, we investigated the spatiotemporal dynamics of fine root biomass (FRB) and its effects on soil chemical properties. The results indicated that the Chinese Fir stump FRB significantly differed with increasing residual time across various soil layers and distances, with an average annual loss rate of 8.40%–9.96%. The living fine root biomass (LFRB) was predominantly concentrated in the 0–20 cm soil layer and decreased with increasing soil depth. Initially, the LFRB was closer to the stumps; however, this proximity effect diminished over time. There were no significant differences in the fine root loss coefficients between layers, within the vertical soil profile with 95% root loss over a time span of 15.1–15.9 years. However, there were horizontal differences, with a 95% root loss over a time span of 13.7–17.0 years. The changes in soil organic matter (SOM) and total nitrogen (STN) content over the study period exhibited a trade-off relationship with the loss of LFRB, with SOM and STN peaking 1 year after the peak of dead fine root biomass (DFRB), suggesting a combined effect of living root exudates and dead root decomposition on SOM and STN enhancement. The trend of LFRB loss was generally inverse to the changes in the soil’s total phosphorus (STP) content, which gradually increased with extended stump retention, indicating that stumps provide a long-term source of phosphorus for the soil. The study also revealed that living fine roots of Chinese Fir stumps can persist in forest soils for a relatively long time and that their biomass dynamics positively affect soil nutrients and carbon storage. These findings provide theoretical support for forest management and suggest that retaining stumps in post-harvest forest management can maintain soil fertility and ecological functions.
Effects of Extrusion Treatment on the Physicochemical and Baking Quality of Japonica Rice Batters and Rice Breads
Authors: He Wenxia , Tang Jingni , Chen Yang , Liu Guanhui , Li Zhenni , Tu Jie , Li Yixuan
Published: January 2025

Gluten-free rice bread made from japonica rice finds challenge in achieving a good shape and structure, presenting a significant obstacle in the baking industry. This study aims to improve the quality of rice bread with japonica rice flour by hot extrusion treatment (without additives). The effects of extrusion on the amylose content, gelatinization degree, hydration capacity, short-range molecular ordering, and microstructure of japonica rice flour were investigated. The results show that the amylose content of the extruded flour increased by 12.43% and the gelatinization degree of it increased by 13.23 times, showing disrupted starch granules, numerous pores, and a better hydration capacity. The addition of extruded flour improved the overall viscoelasticity of the batter. Compared to the control group, the specific volume and porosity of the optimized rice bread were increased by 19.46% and 61.92%, respectively. The gas cell density was increased by 4.63 times, and the average gas cell area of rice bread was reduced by 47.14%. The correlations among the raw material properties of rice flour, the batter properties, and the quality of rice bread products were revealed by principal component analysis. This study demonstrates that the addition of moderate amounts of extruded japonica rice flour could improve the quality of rice bread products.
Shift Work, Psychological Health Disorders, and Job Security Among Nurses: A Cross-Sectional Study
Authors: Alghamdi Rokaya , Bahari Ghareeb
Published: January 2025

Background/Objectives: Shift work is prevalent among nurses, often leading to adverse psychological effects, such as fatigue, depression, anxiety, and stress. Understanding how shift work contributes to psychological health disorders can help healthcare organizations identify critical areas where support should be offered. This study aimed to determine the relationships between shift work, psychological health disorders, and job security among nurses in Saudi Arabia. Methods: This cross-sectional study included 163 nurses, recruited via convenience sampling. The participants completed an online questionnaire that assessed demographic variables, psychological disorders, fatigue, and job security. The data were analyzed using descriptive statistics as well as bivariate analyses to explore relationships between variables. Results: Most participants (73%) worked 12 h shifts, and 67.5% reported fair sleep quality. We found significant correlations among shift work, fatigue, and common psychological disorders. Significant differences were also observed for fatigue (p = 0.007) and depression (p = 0.008). Both nationality (p < 0.001) and shift work (p = 0.015) were correlated with anxiety. Similarly, significant differences were found for nationality (p = 0.001) and shift work (p = 0.002) regarding stress. Conclusions: These findings underscore the psychological challenges faced by nurses related to shift work, emphasizing the importance of addressing fatigue and mental health. Healthcare organizations should implement strategies to enhance job security and support nurses’ well-being to ultimately improve both nurse satisfaction and patient care outcomes. Further research is warranted to explore effective interventions and the long-term effects of shiftwork on nursing professionals.
Evaluating the Adoption of Evidence-Based Management Practices in Eye Hospitals
Authors: Subburaman Ganesh-Babu B. , Gupta Sachin , Ravilla Thulasiraj , Mertens Helen , Webers Carroll A.B. , Biggelaar Frank J.H.M. van den , Zuilen Maaike van , Sundar Balagiri , Merode Frits van
Published: January 2025

Background: Delivering sustainable, high-quality eye care requires a comprehensive understanding of patient conditions, clinical evidence, patients’ preferences, demand patterns, quality supplies, outcomes, financial sustainability, and satisfaction metrics. Evidence-based management (EBM) offers a structured approach to align actions with evidence, enabling effective decision-making and better organizational outcomes. Evaluating current practices against EBM principles fosters awareness and promotes an EBM culture in eye hospitals, supporting improved and sustainable service delivery. Methods: A descriptive cross-sectional survey was conducted in 2023–2024 among 94 eye hospitals worldwide, selected from two networks, using the Centre for Evidence-Based Management (CEBM) assessment questionnaire. Follow-up reminders resulted in 43 responses. Data analysis utilized frequency distributions and Pearson’s correlation to explore relationships between variables. Results: A strong positive correlation was observed between data accessibility and the capacity to interpret data in fostering evidence-based decision-making (r = 0.69, p < 0.01). Additionally, leveraging internal expertise and engaging stakeholders in assessing and utilizing data showed a moderate association with EBM practices (r = 0.48, p < 0.01). Conclusions: Eye hospitals demonstrate alignment with EBM principles, though regional variations exist. Organizations with robust data utilization systems, analytical expertise, and a commitment to continuous improvement are more effective in practicing EBM. Educational and peer-learning initiatives can further support hospitals in adopting EBM principles, strengthening their capacity for evidence-based decision-making, and enhancing eye care services.
Caregivers’ Experience of Supporting Deaf Adults with Mental Health Disorders in Ghana
Authors: Mprah Wisdom Kwadwo , Opoku Maxwell Peprah , Gyimah Ebenezer Mensah , Nur Shakila , Duorinaah Juventus , Frimpomaa Lilian , Efstratopoulou Maria
Published: January 2025

Background: Caregivers play a crucial role in the support and management of individuals experiencing mental health disorders; however, there is a paucity of research concerning the experiences of caregivers of deaf persons diagnosed with any form of mental disorder in Ghana. This study aims to explore the lived experiences of these caregivers, with a particular focus on their perceptions of mental health, available support systems, challenges encountered, and the consequent impact on familial relationships while supporting this underserved population. Method: This qualitative study engaged eleven family caregivers of deaf adults diagnosed with a mental health disorders made up of ten females and one male aged 45–68 years, recruited across four of the sixteen administrative regions in Ghana. Utilizing a semi-structured interview guide, face-to-face interviews were conducted to gather in-depth narratives from the participants. Results: Thematic analysis of the data revealed several key themes, including the awareness and understanding of mental health disorders, availability and accessibility of mental health support services and training, dynamics of psychosocial and familial relationships, and the multifaceted challenges faced in caregiving for individuals with mental health disorders. Conclusion: The findings underscored an urgent need for the development of a specialized caregiving manual tailored for caregivers of deaf individuals experiencing mental health disorders. This study advocates for policymakers to prioritize the creation of such resources to enhance care delivery and improve overall mental health outcomes for this vulnerable population.
Essential Oils as Bioinsecticides Against Blattella germanica (Linnaeus, 1767): Evaluating Its Efficacy Under a Practical Framework
Authors: Manzanares-Sierra Ana , Monsonís-Güell Eduard , Gómez Crisanto , Abril Sílvia , Moreno-Gómez Mara
Published: January 2025

Concerns about the negative effects of traditional insecticides and increasing insecticide resistance have prompted the exploration of botanical alternatives like essential oils (EOs). The registration of biocides is a mandatory procedure, and some regions have established a special status for compounds that meet specific low-risk criteria, which includes certain EOs. This study aimed to evaluate and compare the efficacy of sixteen EOs, both registered as low-risk biocides and/or standard biocides, against the German cockroach, Blattella germanica. A topical application was performed with undiluted EOs (15 µL) on the dorsal surface of awake cockroaches. The results showed significant variations in efficacy, as follows: thyme, sweet orange, and lavender oils were the most effective, achieving 100% mortality within 24 h and a less than 30 s knockdown effect. In contrast, linseed and cottonseed oils were the least effective, resulting in 70% and 40% 24 h mortality. Remarkably, some EOs registered as low-risk biocides performed as well as the ones registered as standard biocides, suggesting that this category does not have to always be at odds with efficacy. Future research should adopt practical, application-driven approaches to ensure bioinsecticides balance performance and safety, meeting both regulatory and consumer demands.
Research on Multi-Time Scale Flexible Resource Aggregation and Evaluation for New Power Systems
Authors: Li Daren , Zhang Qingzhou , Pan Lezhen , Duan Hao , Hong Dongbin , Wu Guiping
Published: January 2025

The strong uncertainty of the high proportion of new energy and the gradual decrease in the proportion of thermoelectric units have led to a shortage of system flexibility resources. System-level energy storage can efficiently alleviate the pressure of peak shaving and frequency regulation. Effective aggregation of flexibility resources is a key technical foundation for enhancing economic operation and advanced user-side response strategies of new power systems. However, the decentralization and heterogeneity of flexibility resources across generation, grid, load, and storage sides pose dual challenges of aggregation speed and accuracy. In view of this, this paper proposes a large-scale multi-dimensional flexibility polymerization method based on different response time scales. First, the flexibility resource definitions and response characteristics of generation, grid, load, and storage sides were analyzed and categorized according to their response time scales. Second, flexibility regulation models for resources on each side were established. On this basis, an improved Minkowski aggregation algorithm is proposed to precisely quantify the regulation capabilities of multi-dimensional flexibility resources at different time scales, enabling efficient resource aggregation. Finally, the results of the case analysis show that the proposed method can accurately aggregate the flexibility resource adjustment capabilities at different time scales to respond to the multi-time scale flexibility requirements of the system.
High-Risk Plaque Characteristics in Patients with Suspected Stable Coronary Artery Disease and Impaired Glucose Tolerance: A Coronary Computed Tomography Angiography Study
Authors: Andersen Thomas Rueskov , Overgaard Katrine Schultz , Heinsen Laurits Juhl , Mohamed Roda Abdulkadir , Madsen Freja Sønder , Precht Helle , Lambrechtsen Jess , Auscher Søren , Egstrup Kenneth
Published: January 2025

Impaired glucose tolerance (IGT), a prediabetic state, is a known risk factor for coronary artery disease (CAD). Low-attenuation plaque (LAP) lesions are associated with a high risk of coronary events. We aimed to evaluate high-risk plaque characteristics in LAP lesions between patients with IGT and normal glucose tolerance (NGT) in patients suspected for stable CAD. Coronary computed tomography angiography (CCTA) identified LAP lesions and assessed plaque volumes, burdens, and high-risk plaque features. Glycemic tolerance was stratified using oral glucose tolerance tests. Among 148 patients, 202 LAP lesions were identified, with 93 patients classified as NGT and 55 as IGT. Patients with IGT had a significantly higher prevalence of LAP lesions compared with NGT (p = 0.007). LAP volume was higher in IGT (16.46 ± 12.52 mm3) compared with NGT (12.66 ± 9.72 mm3, p = 0.01), but this association did not persist in multivariate analysis. The LAP burden was greater in IGT (10.79 ± 6.84%) than NGT (8.62 ± 5.93%, p = 0.02), and the napkin-ring sign was more frequent in IGT (12%) versus NGT (5%, p = 0.02); these associations remained significant in multivariate analysis. Patients with IGT had a higher LAP burden and higher frequency of napkin-ring signs. These findings may help explain the common occurrence of prediabetes in patients with acute myocardial infarction.
Prevalence of Breast Cancer-Related Lymphedema in Israeli Women Following Axillary Procedures
Authors: Kedar Daniel Josef , Zvi Elad , Haran Oriana , Sherker Lior , Sernitski Michael , Oppenheim Nadav , Nizri Eran , Khatib Marian , Barnea Yoav
Published: January 2025

Background/Objectives: Breast cancer-related lymphedema (BCRL) is a chronic complication of breast cancer treatment, leading to physical and psychological morbidity. While widely studied globally, the prevalence and risk factors for BCRL in Israeli patients remain unexplored. This study’s objectives were to determine the prevalence of BCRL in Israeli women treated for breast cancer, validate the Hebrew-translated Norman Questionnaire (NQ) for BCRL screening, and identify risk factors associated with the condition. Methods: A single-center study was conducted at Tel Aviv Sourasky Medical Center, including 181 patients who underwent unilateral axillary lymph node interventions between 2015 and 2018. Participants completed the Hebrew-translated NQ, which was validated through clinical evaluation and circumference-based volume measurements in a subset of 20 patients. Prevalence rates and risk factors were analyzed using multivariate modeling. Results: The prevalence of BCRL was 20%, with rates varying by procedure: 8.9% for sentinel lymph node biopsy, 19.6% for lymph node sampling, and 37.5% for axillary lymph node dissection (ALND). Of the 35 patients with BCRL, only 14% had been previously diagnosed. Risk factors included ALND (OR = 97.31), a higher lymph node excision count (OR = 0.81), and referral to physiotherapy (OR = 133.50). The Hebrew NQ demonstrated strong validity (rs = 0.852; p < 0.001). Conclusions: This is the first study to estimate BCRL prevalence in Israeli women, highlighting underdiagnosis and the need for improved early detection. The Hebrew NQ is a reliable screening tool, enabling timely referral and intervention. Early diagnosis is crucial for optimizing treatment outcomes and improving the quality of life of BCRL patients.
Leveraging Social and Intellectual Capital for Social Entrepreneurship: A Model for Sustainable Business Practices in an Uncertain Environment
Authors: Dixit Krishna , Kumar Pranav , Aashish Kumar , Zohair Mohammad
Published: January 2025

Social entrepreneurship helps solve social issues and bridges public and private sectors. This study uses a comprehensive framework to examine social, intellectual, and external risks in social entrepreneurship and its effects on society and the environment. Using the literature review’s variables, this study developed a conceptual model. The empirical research is based on a survey of 252 social entrepreneurs from different industrial/service sectors. The findings show that intellectual capital helps identify and seize social opportunities. Social capital—social networks, knowledge, trust, and critical resources—guide social entrepreneurs through complex social business environments. The study’s novel approach to external uncertainty in social entrepreneurship shows that firms can design risk-resilient strategies to build sustainable business models by considering external uncertainty. Organizations can consider social entrepreneurship’s social and environmental impacts to create businesses that address the root causes of societal issues. The study adds to theoretical understanding by incorporating a variety of factors that influence social entrepreneurship function and frameworks in driving social and environmental impact.
Physiological and Metabolic Effects of Opuntia ficus indica spp. Peel Formulations
Authors: Arias-Rico José , Hernández-Ortega Iris Cristal , Jaramillo-Morales Osmar Antonio , Cruz-Cansino Nelly del Socorro , Zafra-Rojas Quinatzin Yadira , Flores-Chávez Olga Rocío , Baltazar-Téllez Rosa María , Ramírez-Moreno Esther
Published: January 2025

The objective of this study is to determine the physiological and metabolic effects of administration of dietary fiber formulations to male Wistar rats. The study population was divided into five groups to which food and water were orally administered ad libitum (control), alongside Psyllium plantago, sennosides A and B, cactus pear peel powder, and cactus pear peel tablet powder for 28 days. Body weight, biochemical parameters, fecal moisture, and intestinal transit were determined. The administration of the fiber formulations did not cause differences between the groups and they maintained a healthy weight; however, the consumption of the cactus pear peel tablet powder decreased serum glucose (127.85 ± 5.37 to 68.30 ± 12.48 mg/dL) in rats in a similar form to Psyllium plantago (127.85 ± 5.37 to 96.96 ± 3.26 mg/dL) in comparison with commercial products for rats, and the cactus pear peel powder had lower triglyceride levels (49.52 to 74.44 mg/dL) than commercial products at the end of the treatment. The samples maintained normal HDL levels with the exception of Psyllium plantago that had a decrease in treatment after 28 days. The administration of formulations of dietary fiber of cactus pear peel had physiological and metabolic effects similar to those of commercial products without change in the growth of the animals. Therefore, it could be used in the pharmaceutical or food industry.
Late-Onset HSV-2 Encephalitis in a Kidney Transplant Recipient: A Rare Case Report
Authors: Zjačić Puljiz Danijela , Vrkić Ivana , Jeličić Ivo , Borić Škaro Dijana , Delić Jukić Ivana Kristina , Vicelić Čutura Lučana , Pavičić Ivelja Mirela
Published: January 2025

Infections are an important cause of morbidity and mortality in renal transplant recipients. Among the viral pathogens encountered in this population, herpes simplex virus (HSV), a member of the Alphaherpesvirinae subfamily, has an important place. HSV type 2 infections in this immunosuppressed population are primarily due to viral reactivation. While HSV-2 frequently presents as genital herpes or remains asymptomatic, in rare cases, it can lead to severe neurological manifestations, such as encephalitis, particularly in the early post-transplant period with a reported mortality rate of up to 40%. We present the case of a 49-year-old male who, three years after kidney transplantation, developed acute neurological symptoms, including aphasia and disorientation. Polymerase chain reaction (PCR) analysis of cerebrospinal fluid (CSF) identified HSV-2 as the causative pathogen, enabling a swift and accurate diagnosis. The patient was promptly treated with intravenous acyclovir, adjusted for renal function, resulting in complete neurological recovery and subsequent negative follow-up CSF PCR results. This case emphasizes the vital role of PCR diagnostics as the gold standard for confirming viral encephalitis, particularly in immunosuppressed patients, where atypical presentations can complicate diagnosis. It also highlights the importance of considering HSV-2 encephalitis in the differential diagnosis even beyond the immediate post-transplant period. Early recognition and management, facilitated by the multidisciplinary approach, are critical for improving outcomes in this vulnerable patient population.
Evaluating HPV Viral Load and Multiple Infections for Enhanced Cervical Cancer Risk-Based Assessment
Authors: Varesano Serena , Ciccarese Giulia , Paudice Michele , Mazzocco Katia , Gaggero Gabriele , Ferrero Simone , Icardi Giancarlo , Vellone Valerio Gaetano
Published: January 2025

Human papillomavirus (HPV) is a major cause of cervical cancer, a significant health concern worldwide. Despite advances in screening methods, including the Pap test and the HPV DNA test, limitations remain in accurately predicting which HPV infections will progress to cervical intraepithelial neoplasia (CIN) and, eventually, invasive cancer. This study evaluates the usefulness in real life of assessing HPV viral load and the presence of multiple HPV genotypes in enhancing the diagnostic accuracy of triage in cervical cancer screening. A retrospective analysis was performed on 55 formalin-fixed paraffin-embedded cervical samples collected from women who underwent colposcopy with a biopsy or conization at San Martino Hospital, Genova, Italy, between January and June 2021. Histological diagnoses were compared with molecular analyses (HPV genotyping, viral load quantification and co-infection) using a multiplex real-time PCR platform. Of the samples analyzed, 56.4% were HPV DNA positive, while 40% tested negative. The molecular analysis identified more HPV-negative cases than the histological analysis (p < 0.05). Higher viral loads and HPV co-infections were more frequent in high-grade CIN lesions. These markers may help identify patients at an elevated risk for persistent infections and cancer progression. These findings support the potential of integrating HPV viral load and genotype co-infection assessments into routine cervical cancer screening protocols to improve early detection and reduce overtreatment and unnecessary interventions.
The Risk of Ischemic Stroke in Patients with Chronic Obstructive Pulmonary Disease and Atrial Fibrillation
Authors: Tsai Hsien-Lung , Hsiao Chih-Chun , Chen Yu-Hsuan , Chien Wu-Chien , Chung Chi-Hsiang , Cheng Chun-Gu , Cheng Chun-An
Published: January 2025

Background: Atrial fibrillation (AF) and ischemic stroke (IS) are intricately linked to chronic obstructive pulmonary disease (COPD). Patients who suffer from both COPD and AF demonstrate a 2.85-fold greater risk of IS. However, the long-term risk remains insufficiently explored. Methods: This study utilized data from the Taiwanese National Health Insurance dataset spanning 2000 to 2015. Patients who were newly diagnosed with COPD, identified using the International Classification of Disease, Ninth Revision, Clinical Modification [ICD-9-CM] codes of 491, 492, and 496 and diagnosed with AF (ICD-9-CM code 427.3), were included in the study. The measured events included ISs (ICD-9-CM codes 433–437). Multivariate Cox proportional hazard models were employed to evaluate IS risk factors in this longitudinal analysis. Results: The combined presence of COPD and AF increased the risk of IS, with an adjusted hazard ratio of 5.722 (95% CI: 2.737–8.856, p < 0.001), AF without COPD with an adjusted HR of 3.506 (95% CI: 1.459–5.977, p < 0.001), and COPD with AF with an adjusted HR of 2.215 (95% CI: 1.099–3.538, p < 0.001) compared with patients without COPD and AF. Elderly patients exhibited a greater burden of cardiovascular comorbidities, including obstructive sleep apnea, thus further compounding the risk of IS. Conclusions: The coexistence of COPD and AF was associated with a markedly elevated risk of IS. The result highlights the additive and synergistic contributions of COPD and AF to the risk for IS. Aggressive treatment may mitigate the risk of IS.
Catalytic and Tribological Performances of a Novel Bi-Functional Ionic Liquid in Lubricating Ester Oil
Authors: Wang Yanan , Su Huaigang , Yin Jun , Jiang Cheng , Zhao Qilong , Lou Wenjing , Jia Qian
Published: January 2025

To address the detrimental effects of the residue of catalysts on the tribological performances of ester lubricants, a novel and efficient bi-functional ionic liquid 1-(3,5-di-tert-butyl-4-hydroxybenzyl)-3-methylimidazole di(2-ethylhexyl) phosphate ([(BHT-1)MIM][DEHP]) was prepared. The catalyst not only facilitates the synthesis of pentaerythritol tetra-hexanoate (PETH) through the catalytic esterification reaction—achieving up to 96% conversion with a 94% yield—but also enhances the tribological performance of ester oil PETH when used as a lubricant additive. The tribological property has been improved remarkably: the mean friction coefficient for PETH + [(BHT-1)MIM][DEHP] is notably lower, at 0.110, compared to the PETH, which has a coefficient of 0.180. Meanwhile, the wear scar diameter of the steel ball, when lubricated with PETH + [(BHT-1)MIM][DEHP], is notably smaller than that of a steel ball lubricated solely with PETH. Especially, the reduction in the wear volume at 100 °C is up to 81.46% compared with the base oil PETH. [(BHT-1)MIM][DEHP], PETH + [(BHT-1)MIM][DEHP], and the worn track of the upper running ball and lower disc were systematically characterized by using Nuclear Magnetic Resonance (NMR) spectra, a Fourier Transform Infrared Spectrometer (FT-IR), a field emission scanning electron microscope (FESEM), Thermal gravity analysis (TG), X-ray photoelectron spectroscopy (XPS), and an optical microscope (OM). The wear mechanism of the tailored lubricant oil was discussed in terms of the chemical composition of the worn surface.
Exploring Construction of Biomedical Ti6Al4V-Ti5Cu Composite Alloy with Interpenetrating Structure: Microstructure and Corrosion Resistance
Authors: Zhou Yuan , Zhao Qing , Hong Ruchen , Mai Dongyi , Lu Yanjin , Lin Jinxin
Published: January 2025

Cu-bearing titanium alloys exhibit promising antibacterial properties for clinical use. A novel Ti6Al4V-Ti5Cu composite alloy is developed using powder bed fusion (selective laser sintering, SLM) and spark plasma sintering (SPS). SLM produces a triple periodic minimal surface (TPMS) lattice structure from Ti6Al4V, which is then filled with Ti-5Cu powders and sintered using SPS. Microstructural analysis confirms a well-bonded interface between Ti6Al4V and Ti-5Cu could be achieved through SLM-SPS technology. The composite primarily showcases laths α phase, with Ti2Cu precipitates in the Ti-5Cu region. Electrochemical assessments reveal superior corrosion resistance in the Ti6Al4V-Ti5Cu composite compared to SLM-Ti6Al4V and SPS-Ti-5Cu. The antibacterial rate of the TPMS structure exceeds 90%, and that of TCCU-90 reaches as high as 99%, manifesting robust antibacterial activity. These findings suggest a strategy for creating biomimetic alloys that seamlessly combine structure and multifunctionality within biomedical materials.
Recent Advances in Barnacle-Inspired Biomaterials in the Field of Biomedical Research
Authors: Min Tiantian , Zhang Zhongna , Chen Lan , Li Jingan
Published: January 2025

As a marine fouling organism, barnacles secrete a cement whose proteins self-assemble into stable nanofibers, conferring exceptional underwater adhesion and curing properties. The barnacle cement proteins (BCPs) are of significant interest in biomedicine due to their adhesiveness, water resistance, stability, and biocompatibility, making them ideal for developing novel biomaterials. Additionally, BCPs have wound-healing acceleration and antibacterial properties, offering new insights for antimicrobial biomaterial development. Recently, barnacle-inspired materials have seen extensive research and notable progress in biomedicine. As the understanding of barnacle cement and its adhesion mechanisms deepens, their medical applications are expected to expand. This review summarizes the latest advancements of barnacle biomimetic materials in biomedicine, including their use in adhesives, tissue engineering, drug delivery, and hemostasis, highlighting their characteristics, applications, and potential research directions, and providing a comprehensive reference for the field.
Mathematical Modelling of Viscoelastic Media Without Bulk Relaxation via Fractional Calculus Approach
Authors: Shitikova Marina V. , Modestov Konstantin A.
Published: January 2025

In the present paper, several viscoelastic models are studied for cases when time-dependent viscoelastic operators of Lamé’s parameters are represented in terms of the fractional derivative Kelvin–Voigt, Scott-Blair, Maxwell, and standard linear solid models. This is practically important since precisely these parameters define the velocities of longitudinal and transverse waves propagating in three-dimensional media. Using the algebra of dimensionless Rabotnov’s fractional exponential functions, time-dependent operators for Poisson’s ratios have been obtained and analysed. It is shown that materials described by some of such models are viscoelastic auxetics because the Poisson’s ratios of such materials are time-dependent operators which could take on both positive and negative magnitudes.
Blockade of the STAT3/BCL-xL Axis Leads to the Cytotoxic and Cisplatin-Sensitizing Effects of Fucoxanthin, a Marine-Derived Carotenoid, on Human Bladder Urothelial Carcinoma Cells
Authors: Dai Wen-Chyi , Chen Tzu-Hsuan , Peng Tzu-Ching , He Yung-Ching , Hsu Chao-Yu , Chang Chia-Che
Published: January 2025

Bladder cancer is a globally prevalent urological malignancy, with transitional carcinoma (TCC) representing the majority of cases. Cisplatin is the primary drug for metastatic bladder cancer chemotherapy; however, its application is limited by nephrotoxicity and resistance. Signal Transducer and Activator of Transcription 3 (STAT3) is an oncogenic transcription factor often overactivated in various cancers, making it an appealing drug target. Fucoxanthin, a marine carotenoid, has significant anticancer properties. This study explored Fucoxanthin’s cytotoxic effects and its potential to potentiate the efficacy of Cisplatin, along with the mechanisms underlying these effects, on human bladder TCC cells. We demonstrated that Fucoxanthin is cytotoxic to bladder TCC cells by inducing apoptosis, evidenced by z-VAD-fmk-mediated annulment of Fucoxanthin’s cytotoxicity. Furthermore, Fucoxanthin reduced the levels of inherent or interleukin-6-induced tyrosine 705-phosphorylated STAT3 accompanied by downregulating BCL-xL, a well-established STAT3 target. Notably, ectopic expression of STAT3-C, a dominant-active STAT3 mutant, or BCL-xL thwarted Fucoxanthin’s proapoptotic and cytotoxic actions. Moreover, Fucoxanthin at subtoxic dosages enhanced the susceptibility to Cisplatin-induced apoptosis of bladder TCC cells initially resistant to Cisplatin. Remarkably, this Cisplatin-sensitizing effect of Fucoxanthin was abrogated when cells ectopically expressed STAT3-C or BCL-xL. Overall, for the first time, we proved that the proapoptotic, cytotoxic, and Cisplatin-sensitizing effects of Fucoxanthin on human bladder TCC cells are attributed to the blockade of the STAT3/BCL-xL axis. Our findings highlight that targeting the STAT3/BCL-xL axis is a promising strategy to eliminate bladder TCC cells and facilitate Cisplatin sensitization, and further support the potential of incorporating Fucoxanthin into Cisplatin-based chemotherapy for treating bladder cancer.
Impact of Exercise Manual Program on Biochemical Markers in Sedentary Prediabetic Patients: A Randomized Controlled Trial
Authors: Hafeez Sana , Rehman Syed Shakil Ur , Riaz Saima , Hafeez Imran , Hafeez Zarwa , Mumtaz Hassan
Published: January 2025

Background and Objectives: Prediabetes is a medical disease characterized by elevated blood sugar levels that exceed normal levels but do not meet the criteria for a diagnosis of diabetes mellitus. This study aimed to assess the impact of structured exercise manual interventions on the biochemical markers of sedentary prediabetic patients over sixteen weeks. Materials and Methods: A sixteen-weeks randomized controlled trial was conducted to assess the impact of an exercise-based manual program on biochemical markers, such as HbA1c, insulin sensitivity measures, and lipid profiles, in sedentary individuals with prediabetes. The Riphah Rehabilitation Center in Lahore, Pakistan, was the site of the trial. In this investigation, 126 individuals with prediabetes were randomly assigned to three groups: control, unsupervised, and supervised. The RCT was completed by 36 participants in each group after a 16-weeks intervention in the supervised and unsupervised groups, as well as a follow-up in the control group. An activity-based exercise manual that included dietary guidelines, educational materials, and an exercise routine was followed by both the supervised and unsupervised groups. The exercise interventions included both aerobic and resistance components. Results: The results indicated that the supervised group exhibited a substantial increase in insulin sensitivity, lipid profiles, and glycemic control when contrasted with the unsupervised and control groups. Significant improvements were observed in key biochemical parameters, including fasting blood levels (supervised as compared to unsupervised and control, respectively, the mean difference was 12.82 mg/dL vs. 11.36 mg/dL vs. 0.09 mg/dL p > 0.001), HbA1c (supervised as compared to unsupervised and control groups, respectively, the mean difference was 0.67% vs. 0.69% vs. 0.13% p < 0.001), and lipid profile (triglycerides (mean difference 0.25 mmol/L, 0.08 mmol/L, 0.11 mmol/L p < 0.001); LDL (mean difference 19.31 mg/dL, 10.51 mg/dL, 2.49 mg/dL p < 0.001); HDL (mean difference −12.68 mg/dL, −8.03 mg/dL, −1.48 mg/dL p < 0.001)). In comparison to the unsupervised and control groups, the insulin sensitivity parameters also demonstrated a modest improvement in the supervised group. The supervised group exhibited the greatest benefits from exercise among the groups that received exercise interventions. Conclusions: The present investigation demonstrated the significance of including structured physical activity into the regular routine of individuals with prediabetes, to decelerate the advancement of prediabetes to type 2 diabetes mellitus (T2DM). The current study emphasizes the essential role of structured exercise routines in the control of prediabetes and suggests that monitoring enhances the adherence and effectiveness of lifestyle interventions.
A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries
Authors: Khan Mohammad Shehryaar , Wurzbacher Christian , Uchaikina Anna , Pleshkov Boris , Mirshina Olga , Drewes Jörg E.
Published: January 2025

Geographical variations in infectious diseases create differences in public health priorities between high- and low-income countries. Low- and middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those to detect SARS-CoV-2, offers LMICs a promising tool for monitoring pathogens of local concern. In this work, we summarize important wastewater biomarkers for LMICs and their associated public health challenges, ranging from pathogens causing gastroenteritis to putative markers for plant diseases linked to food safety, as well as antimicrobial resistance. We raise awareness of the great potential of WBE for LMICs and highlight the critical health markers, research needs, and strategies necessary to establish tailored wastewater surveillance programs.
Anti-Inflammatory and Antidiarrheal Effects of Two Strains of Lactic Acid Bacteria Isolated from Healthy Pets on Escherichia coli K88-Induced Diarrhea in Mice
Authors: Zhao Ya , Liang Shukun , Fu Xiaoxin , Guo Yaping , Wang Yu , Wang Jiaxue , Wang Xiumin , Wang Zhenlong , Tao Hui , Han Bing , Wang Jinquan
Published: January 2025

Lactic acid bacteria play a crucial role in maintaining the health of the host’s gut microbiota. In this study, the anti-inflammatory properties of Limosilactobacillus reuteri LR20-6 and Lacticplantibacillus plantarum L272 were evaluated using a mouse model of diarrhea induced by Escherichia coli. We also investigated their effects on gut microbiota regulation. The results indicated that both Lacticplantibacillus plantarum and Limosilactobacillus reuteri could reduce inflammation by inhibiting the expression of inflammatory factors IL-6 and TNF-α and blocking the MyD88 and NF-kB/p65 signaling pathways. Additionally, after intervention with these strains, the relative abundance of Lactobacillus was significantly increased. This suggested that Lacticplantibacillus plantarum and Limosilactobacillus reuteri could mitigate the severity of E. coli-induced diarrhea and enhance the abundance of beneficial probiotics in the gut of animals.
Terminal Fan Deposition and Diagenetic Control in the Lower Paleogene of the Shahejie Formation, Bonan Sag, Bohai Basin, China: Insights into Reservoir Quality
Authors: Mioumnde Arthur Paterne , Zhang Liqiang , Yan Yiming , Quaye Jonathan Atuquaye , Zebaze Kevin Mba , Sedziafa Victor , Bapowa Carole Laouna , Zafar Zeeshan , Room Shahab Aman e
Published: January 2025

In the Bonan area, the lower fourth member of the Shahejie Formation (Es4x) is buried beneath a sedimentary pile ranging from 2500 to 5000 m. Understanding the impact of diagenetic alterations on these deeply buried reservoirs is crucial for effective hydrocarbon exploration and production. This study employs a terminal fan sedimentation model, encompassing depositional environments such as feeder channels, distributary channels, floodplains, and basinal zones, to provide insights into the spatial distribution of reservoir properties and their influence on the localization of optimal reservoirs within the sag. The analysis integrates diagenetic facies with well log responses, subsurface porosity trends, and permeability variations across the formation. The petrographic analysis indicates that the sandstone is composed primarily of litharenite, feldspathic litharenite, lithic arkose, and minor amounts of arkose. The dominant clay cement is illite, accompanied by mixed-layer smectite/illite, chlorite, and kaolinite. Thin section observations reveal secondary porosity formed through the dissolution of quartz grains, volcanic rock fragments, and feldspar, along with their associated cements. These sandstones exhibit relatively good sorting, with average porosity and air permeability values of 14.01% and 12.73 mD, respectively. Diagenetic alterations are categorized into three processes: porosity destruction, preservation, and generation. Key diagenetic mechanisms include compaction, cementation, replacement, and dissolution, with compaction exerting the most significant control on reservoir porosity reduction. Statistical analysis indicates that the average porosity loss due to compaction is approximately 13.3%, accounting for about 38% of the original porosity. The detrital rock cement predominantly comprises quartz (42%), feldspar (32%), clay minerals (14%), and carbonate (12%). Under the prevailing depositional conditions, porosity is enhanced by dissolution and fracturing, while late-stage diagenetic cementation by clay and carbonate minerals—excluding chlorite—adversely affects reservoir quality. Consequently, the distributary zone is identified as the primary target for exploration.