bluuebunny/crossref_metadata_2025 huggingface dataset:
A Numerical Investigation to Determine the p–y Curves of Laterally Loaded Piles
Authors: Yin Kexin , Li Lianghui , Di Filippo Eugenia
Published: November 2021

This paper focuses on a numerical approach to finding the p–y curves for laterally loaded piles. The Drucker–Prager plastic model is employed and implemented within a finite element MATLAB code. The pre- and post-processing code for Gmsh and related numerical tools are established as well. The p–y curve results from this new approach have been validated and compared to the typical design equations of API (American Petroleum Institute) and Matlock. The validation reveals that the code leads to lower p–y curves than the API and Matlock equations when the horizontal displacement is less than 0.35 times the diameter of the pile (B). A sensitivity analysis of the number of elements and the interface thickness is presented. The results indicate that the obtained p–y curves are independent of the two factors. Finally, the influence of clay content on the p–y behavior is investigated by the implemented MATLAB code. When y < 0.15B, the same lateral capacity values are resulted at clay contents of 27.5% and 55%, and they are higher than the ones for 0% clay content. The p–y curves show a decreasing trend with increasing clay content after y > 0.15B.
Bone Marrow Aspirate Concentrate versus Platelet Rich Plasma or Hyaluronic Acid for the Treatment of Knee Osteoarthritis
Authors: Dulic Oliver , Rasovic Predrag , Lalic Ivica , Kecojevic Vaso , Gavrilovic Gordan , Abazovic Dzihan , Maric Dusan , Miskulin Mladen , Bumbasirevic Marko
Published: November 2021

Background: In the last decade, regenerative therapies have become one of the leading disease modifying options for treatment of knee osteoarthritis (OA). Still, there is a lack of trials with a direct comparison of different biological treatments. Our aim was to directly compare clinical outcomes of knee injections of Bone Marrow Aspirate Concentrate (BMAC), Platelet-rich Plasma (PRP), or Hyaluronic acid (HA) in the OA treatment. Methods: Patients with knee pain and osteoarthritis KL grade II to IV were randomized to receive a BMAC, PRP, and HA injection in the knee. VAS, WOMAC, KOOS, and IKDC scores were used to establish baseline values at 1, 3, 6, 9, and 12 months. All side effects were reported. Results: A total of 175 patients with a knee osteoarthritis KL grade II-IV were randomized; 111 were treated with BMAC injection, 30 with HA injection, and 34 patients with PRP injection. There were no differences between these groups when considering KL grade, BMI, age, or gender. There were no serious side effects. The mean VAS scores after 3, 7, 14, and 21 days showed significant differences between groups with a drop of VAS in all groups but with a difference in the BMAC group in comparison to other groups (p < 0.001). There were high statistically significant differences between baseline scores and those after 12 months (p < 0.001) in WOMAC, KOOS, KOOS pain, and IKDC scores, and in addition, there were differences between these scores in the BMAC group in comparison with other groups, except for the PRP group in WOMAC and the partial IKDC score. There were no differences between the HA and PRP groups, although PRP showed a higher level of clinical improvement. Conclusions: Bone marrow aspirate concentrate, Leukocyte rich Platelet Rich Plasma, and Hyaluronic acid injections are safe therapeutic options for knee OA and provide positive clinical outcomes after 12 months in comparison with findings preceding the intervention. BMAC could be better in terms of clinical improvements in the treatment of knee OA than PRP and HA up to 12 months. PRP provides better outcomes than HA during the observation period, but these results are not statistically significant. More randomized controlled trials and high quality comparative studies are needed for direct correlative conclusions.
Clinical Sign-Based Rapid Response Team Call Criteria for Identifying Patients Requiring Intensive Care Management in Japan
Authors: Okawa Reiko , Yokono Tomoe , Koyama Yu , Uchiyama Mieko , Oono Naoko
Published: November 2021

Background and Objectives: For effective function of the rapid response system (RRS), prompt identification of patients at a high risk of cardiac arrest and RRS activation without hesitation are important. This study aimed to identify clinical factors that increase the risk of intensive care unit (ICU) transfer and cardiac arrest to identify patients who are likely to develop serious conditions requiring ICU management and appropriate RRS activation in Japan. Materials and Methods: We performed a single-center, case control study among patients requiring a rapid response team (RRT) call from 2017 to 2020. We extracted the demographic data, vital parameters, blood oxygen saturation (SpO2) and the fraction of inspired oxygen (FiO2) from the medical records at the time of RRT call. The patients were divided into two groups to identify clinical signs that correlated with the progression of clinical deterioration. Patient characteristics in the two groups were compared using statistical tests based on the distribution. Receiver operating characteristic (ROC) curve analysis was used to identify the appropriate cut-off values of vital parameters or FiO2 that showed a significant difference between-group. Multivariate logistic regression analysis was used to identify patient factors that were predictive of RRS necessity. Results: We analyzed the data of 65 patients who met our hospital’s RRT call criteria. Among the clinical signs in RRT call criteria, respiratory rate (RR) (p < 0.01) and the needed FiO2 were significantly increased (p < 0.01) in patients with severe disease course. ROC curve analysis revealed RR and needed FiO2 cut-off values of 25.5 breaths/min and 30%. The odds ratio for the progression of clinical deterioration was 40.5 times higher with the combination of RR ≥ 26 breaths/min and needed FiO2 ≥ 30%. Conclusions: The combined use of RR ≥ 26 breaths/min and needed FiO2 ≥ 30% might be valid for identifying patients requiring intensive care management.
Feature Importance of Acute Rejection among Black Kidney Transplant Recipients by Utilizing Random Forest Analysis: An Analysis of the UNOS Database
Authors: Thongprayoon Charat , Jadlowiec Caroline C. , Leeaphorn Napat , Bruminhent Jackrapong , Acharya Prakrati C. , Acharya Chirag , Pattharanitima Pattharawin , Kaewput Wisit , Boonpheng Boonphiphop , Cheungpasitporn Wisit
Published: November 2021

Background: Black kidney transplant recipients have worse allograft outcomes compared to White recipients. The feature importance and feature interaction network analysis framework of machine learning random forest (RF) analysis may provide an understanding of RF structures to design strategies to prevent acute rejection among Black recipients. Methods: We conducted tree-based RF feature importance of Black kidney transplant recipients in United States from 2015 to 2019 in the UNOS database using the number of nodes, accuracy decrease, gini decrease, times_a_root, p value, and mean minimal depth. Feature interaction analysis was also performed to evaluate the most frequent occurrences in the RF classification run between correlated and uncorrelated pairs. Results: A total of 22,687 Black kidney transplant recipients were eligible for analysis. Of these, 1330 (6%) had acute rejection within 1 year after kidney transplant. Important variables in the RF models for acute rejection among Black kidney transplant recipients included recipient age, ESKD etiology, PRA, cold ischemia time, donor age, HLA DR mismatch, BMI, serum albumin, degree of HLA mismatch, education level, and dialysis duration. The three most frequent interactions consisted of two numerical variables, including recipient age:donor age, recipient age:serum albumin, and recipient age:BMI, respectively. Conclusions: The application of tree-based RF feature importance and feature interaction network analysis framework identified recipient age, ESKD etiology, PRA, cold ischemia time, donor age, HLA DR mismatch, BMI, serum albumin, degree of HLA mismatch, education level, and dialysis duration as important variables in the RF models for acute rejection among Black kidney transplant recipients in the United States.
Effect of Deformation Structure of AISI 316L in Low-Temperature Vacuum Carburizing
Authors: Cheon Hyunseok , Kim Kyu-Sik , Kim Sunkwang , Heo Sung-Bo , Lim Jae-Hun , Kim Jun-Ho , Yoon Seog-Young
Published: November 2021

The effect of plastic deformation applied to AISI 316L in low-temperature vacuum carburizing without surface activation was investigated. To create a difference in the deformation states of each specimen, solution and stress-relieving heat treatment were performed using plastically deformed AISI 316L, and the deformation structure and the carburized layer were observed with EBSD and OM. The change in lattice parameter was confirmed with XRD, and the natural oxide layers were analyzed through TEM and XPS. In this study, the carburized layer on the deformed AISI 316L was the thinnest and the dissolved carbon content of the layer was the lowest. The thickness and composition of the natural oxide layer on the surface were changed due to the deformed structure. The natural oxide layer on the deformed AISI 316L was the thickest, and the layer was formed with a bi-layer structure consisting of an upper Cr-rich layer and a lower Fe-rich layer. The thick and Cr-rich oxide layer was difficult to decompose due to the requirement for lower oxygen partial pressure. In conclusion, the oxide layer is the most influential factor, and its thickness and composition may determine carburizing efficiency in low-temperature vacuum carburizing without surface activation.
Temperature Field-Assisted Ultraviolet Nanosecond Pulse Laser Processing of Polyethylene Terephthalate (PET) Film
Authors: Xu Jun , Rong Youmin , Liu Weinan , Zhang Tian , Xin Guoqiang , Huang Yu , Wu Congyi
Published: November 2021

Understanding the mechanism of and how to improve the laser processing of polymer films have been important issues since the advent of the procedure. Due to the important role of a photothermal mechanism in the laser ablation of polymer films, especially in transparent polymer films, it is both important and effective to adjust the evolution of heat and temperature in time and space during laser processing by simply adjusting the ambient environment so as to improve and understand the mechanism of this procedure. In this work, studies on the pyrolysis of PET film and on temperature field-assisted ultraviolet nanosecond (UV-ns) pulse laser processing of polyethylene terephthalate (PET) film were performed to investigate the photothermal ablation mechanism and the effects of temperature on laser processing. The results showed that the UV-ns laser processing of PET film was dominated by the photothermal process, in which PET polymer chains decomposed, melted, recomposed and reacted with the ambient gases. The ambient temperature changed the heat transfer and temperature distribution in the laser processing. Low ambient temperature reduced the thermal effect and an increase in ambient temperature improved its efficiency (kerf width: 39.63 μm at −25 °C; 48.30 μm at 0 °C; 45.81 μm at 25 °C; 100.70 μm at 100 °C) but exacerbated the thermal effect.
Secondary Metabolite Production and Terpenoid Biosynthesis in Endophytic Fungi Cladosporium cladosporioides Isolated from Wild Cymbopogon martinii (Roxb.) Wats
Authors: Jayaram Hemalatha , Marigowda Vinutha , Thara Saraswathi Kunigal Jagadishchandra
Published: November 2021

Endophytic fungi Cladosporium cladosporioides (F1-MH810309) and Cladosporium tenuissimum (F2-MN715834) from the leaf of wild Cymbopogon martinii (MT90507) were isolated and selected based on the persistent occurrence during different seasons of the year. They were identified based on the morphological features and molecular characterization (ITS sequence), and later deposited at NCBI. Phytochemical studies on F1, F2 and host extracts showed the presence of alkaloids, flavonoids, phenols, terpenoids and tannins. The GC-MS of F1 extract (control) under the axenic condition revealed compounds like hexadecane, heptadecane,2,4-Ditert-butylphenol, E-14 hexadecenal, geraniol, geranyl acetate and cubenol similar to the host. The GC-MS of F2 extract (control) revealed metabolites that were unique. Further, both F1 and F2 were cultured in the supplementation of different concentrations (5%, 10%, 15% and 20%) of the host plant extract (an-axenic condition). The GC-MS of F1 extracts (test) exhibited good growth and showed the gradual increased production of terpenoid compounds whereas the F2 (test) did not show any growth. These compounds such as hyrdoxymenthol, nor-borneol, cedralacetate, α-cyclogeraniol, campesterol, β-cyclogeraniol, linalool oxide,2,3-boranediol, citronellyltiglate and 2,3-pinanediol were produced in a minor quantity and were known as biotransformed forms of the precursor compounds present in the host extract. In comparison, only F1 was able to produce terpenoids similar to the host species both in axenic and an-axenic conditions. Hence from the current study, the endophytic fungus F1 isolated from wild C. martinii for the first time can serve as a better resource for the bioprospection of an important terpenoid and its metabolites.
Innovative Development of Batch Dyed 3D Printed Acrylonitrile/Butadiene/Styrene Objects
Authors: Kutnjak-Mravlinčić Suzana , Sutlović Ana , Glogar Martinia Ira , Ercegović Ražić Sanja , Godec Damir
Published: November 2021

According to the great impact of additive technology on the development of modern industry, a lot of research is being done to obtain 3D printed parts with better properties. This research is extremely important because there are no scientific papers in the field of post dyeing of acrylonitrile/butadiene/styrene (ABS) 3D printed parts. The experiment was carried out using disperse dyes on ABS specimens. The obtained coloration of the specimens was in the primary colors (yellow, red, and blue) in the specified dyestuff concentration range and was evaluated using an objective CIELab system. Based on the obtained color parameters, remission values and Kubelka-Munk coefficient, dye mixtures and an ombre effect were performed to obtain patterns in the desired hues. Abrasion resistance of disperse dyed specimens was tested using different abrasive materials over a wide range of fineness to simulate different indoor and outdoor soils and was compared to abrasion resistance of specimens produced from the industrially dyed wire with the master batch. The results show that 3D printed ABS products can be produced in one or more desired shades with satisfactory abrasion resistance. This undoubtedly represents the added value of 3D printed ABS parts and extends their application to the field of creative industries and design, specifically footwear design.
Gas Chromatographic Fingerprint Analysis for the Comparison of Seized Cannabis Samples
Authors: Slosse Amorn , Van Durme Filip , Samyn Nele , Mangelings Debby , Vander Heyden Yvan
Published: November 2021

Cannabis sativa L. is widely used as recreational illegal drugs. Illicit Cannabis profiling, comparing seized samples, is challenging due to natural Cannabis heterogeneity. The aim of this study was to use GC–FID and GC–MS herbal fingerprints for intra (within)- and inter (between)-location variability evaluation. This study focused on finding an acceptable threshold to link seized samples. Through Pearson correlation-coefficient calculations between intra-location samples, ‘linked’ thresholds were derived using 95% and 99% confidence limits. False negative (FN) and false positive (FP) error rate calculations, aiming at obtaining the lowest possible FP value, were performed for different data pre-treatments. Fingerprint-alignment parameters were optimized using Automated Correlation-Optimized Warping (ACOW) or Design of Experiments (DoE), which presented similar results. Hence, ACOW data, as reference, showed 54% and 65% FP values (95 and 99% confidence, respectively). An additional fourth root normalization pre-treatment provided the best results for both the GC–FID and GC–MS datasets. For GC–FID, which showed the best improved FP error rate, 54 and 65% FP for the reference data decreased to 24 and 32%, respectively, after fourth root transformation. Cross-validation showed FP values similar as the entire calibration set, indicating the representativeness of the thresholds. A noteworthy improvement in discrimination between seized Cannabis samples could be concluded.
Sustainable Hues: Exploring the Molecular Palette of Biowaste Dyes through LC-MS Metabolomics
Authors: Molino Ralph John Emerson J. , Rellin Klidel Fae B. , Nellas Ricky B. , Junio Hiyas A.
Published: November 2021

Underutilized biowaste materials are investigated for their potential as sustainable textile colorants through an approach based on mass spectrometry, bioinformatics, and chemometrics. In this study, colorful decoctions were prepared from the outer bark of Eucalyptus deglupta and fruit peels of Syzygium samarangense, Syzygium malaccense, Diospyros discolor, and Dillenia philippinensis. Textile dyeing was performed along with liquid chromatography–mass spectrometry (LC–MS)-based untargeted metabolomics to determine the small molecules responsible for the observed colors. Global Natural Products Social Molecular Networking (GNPS) guided the annotation of black-producing proanthocyanidins in D. philippinensis and E. deglupta through complexation with FeSO4 mordant. Flavonoids from the yellow-colored D. philippinensis extracts were found to be similar to those in Terminalia catappa, a known traditional dye source. A higher intensity of epicatechin in E. deglupta produced a red-brown color in the presence of Cu2+. Furthermore, Syzygium fruit peels have poor wash-fastness in cotton fibers, but bioactive chalcone unique to S. samarangense samples may be a potential nutritional food colorant. Unsupervised PCA and supervised OPLS-DA chemometrics distinguished chemical features that affect dyeing properties beyond the observed color. These findings, along with growing data on natural dyes, could guide future research on sustainable colorants.
Synthesis of Metastable Ternary Pd-W and Pd-Mo Transition Metal Carbide Nanomaterials
Authors: Thode James M. , Harris Daniel P. , Wan Cheng , Leonard Brian M.
Published: November 2021

Research and catalytic testing of platinum group transition metal carbides have been extremely limited due to a lack of reliable, simple synthetic approaches. Powder samples have been reported to phase separately above 1%, and only thin-film samples have been reported to have appreciable amounts of precious metal doping. Herein, we demonstrated, through the simple co-precipitation of Pd and W or Mo precursors and their subsequent annealing, the possibility to readily form ternary carbide powders. During the investigation of the Pd-W ternary system, we discovered a new hexagonal phase, (PdW)2C, which represents the first non-cubic Pd ternary carbide. Additionally, the solubility of Pd in the Pd-W-C and Pd-Mo-C systems was increased to 24 and 32%, respectively. As a potential application, these new materials show an enhanced activity for the methanol oxidation reaction (MOR) compared to industrial Pd/C.
Particle Surface Modification in the Near-Electrode Region of an RF Discharge
Authors: Kononov Evgenii Aleksandrovich , Vasiliev Mikhail Mikhailovich , Vasilieva Elena Valeryevna , Petrov Oleg Fedorovich
Published: November 2021

The results of a study on particles’ surfaces after being exposed to the near-electrode region of a radio frequency (RF) discharge are presented. It was experimentally displayed that metal starts being deposited on the surface of particles levitating above the lower electrode of the discharge chamber after switching the RF discharge on. For melamine-formaldehyde (MF) particles, the appearance of an island metal coating is observed after 30 min of plasma exposure. Eroded electrodes and elements of the gas discharge chamber may serve as a source of deposited material. In addition, an analysis of the surface and composition of particles placed on the upper electrode after 6 h of plasma exposure is presented. We reveal that the composition and structure of the particle coating changes during the experiment. The MF particles under exposure become eroded, and needle-like structures containing metals are formed on their surface. We also observe the formation of columnar structures from the products of erosion of electrodes on particles with a metal coating.
Uptake and Accumulation of Nano/Microplastics in Plants: A Critical Review
Authors: Azeem Imran , Adeel Muhammad , Ahmad Muhammad Arslan , Shakoor Noman , Jiangcuo Gama Dingba , Azeem Kamran , Ishfaq Muhammad , Shakoor Awais , Ayaz Muhammad , Xu Ming , Rui Yukui
Published: November 2021

The ubiquitous presence of microplastics (MPs) and nanoplastics (NPs) in the environment is an undeniable and serious concern due to their higher persistence and extensive use in agricultural production. This review highlights the sources and fate of MPs and NPs in soil and their uptake, translocation, and physiological effects in the plant system. We provide the current snapshot of the latest reported studies with the majority of literature spanning the last five years. We draw attention to the potential risk of MPs and NPs in modern agriculture and their effects on plant growth and development. We also highlight their uptake and transport pathways in roots and leaves via different exposure methods in plants. Conclusively, agricultural practices, climate changes (wet weather and heavy rainfall), and soil organisms play a major role in transporting MPs and NPs in soil. NPs are more prone to enter plant cell walls as compared to MPs. Furthermore, transpiration pull is the dominant factor in the plant uptake and translocation of plastic particles. MPs have negligible negative effects on plant physiological and biochemical indicators. Overall, there is a dire need to establish long-term studies for a better understanding of their fate and associated risks mechanisms in realistic environment scenarios for safe agricultural functions.
APOE ɛ4 Is Associated with Postprandial Inflammation in Older Adults with Metabolic Syndrome Traits
Authors: Schönknecht Yannik Bernd , Crommen Silke , Stoffel-Wagner Birgit , Coenen Martin , Fimmers Rolf , Stehle Peter , Ramirez Alfredo , Egert Sarah
Published: November 2021

The apolipoprotein E (APOE) polymorphism impacts blood lipids and biomarkers of oxidation and inflammation, contributing to an isoform-dependent disease risk. We investigated the effect of the APOE genotype on postprandial metabolism after consumption of three different isoenergetic (4200 kJ) meals in older adults with a CVD risk phenotype. In a randomized crossover study, participants with metabolic syndrome traits (APOE E3, n = 39; E4, n = 10; mean age, 70 ± 5 years; BMI 31.3 ± 3.0 kg/m2) consumed a Western-like diet high-fat (WDHF), Western-like diet high-carbohydrate (WDHC), or Mediterranean-like diet (MED) meal. Parameters of lipid and glucose metabolism, inflammatory, and oxidative parameters were analyzed in blood samples collected at fasting and 1–5 h postprandially. Data were analyzed by linear mixed models. The magnitude of the IL-6 increase after the WDHF meal was significantly higher in E4 than in E3 carriers (iAUC: E4 = 7.76 vs. E3 = 2.81 pg/mL × h). The time to detect the IL-6 increase was shorter in the E4 group. All meals produced postprandial glycemia, insulinemia, and lipidemia, without differences between the E3 and the E4 groups. IL-1β and oxidized LDL levels did not change postprandially. In conclusion, APOE E4 carriers display increased postprandial inflammation, indicated by higher postprandial IL-6 increase, when compared to non-carriers.
Metabolically Healthy Obesity (MHO) vs. Metabolically Unhealthy Obesity (MUO) Phenotypes in PCOS: Association with Endocrine-Metabolic Profile, Adherence to the Mediterranean Diet, and Body Composition
Authors: Barrea Luigi , Muscogiuri Giovanna , Pugliese Gabriella , de Alteriis Giulia , Colao Annamaria , Savastano Silvia
Published: November 2021

Obesity and obesity-related low-grade inflammation are common findings in polycystic ovary syndrome (PCOS), the most common endocrine-metabolic disorder-affecting women in reproductive age. The terms metabolically healthy obese (MHO), and metabolically unhealthy obese (MUO) have been introduced to define individuals with obesity in whom cardio-metabolic risk factors are absent or present, respectively. To date, evidence investigating differences in body composition and adherence to the Mediterranean diet (MD) between MHO and MUO-PCOS women are lacking. Aim of this study was to better characterize the determinants of the metabolic health status in PCOS patients with obesity according to MHO and MUO phenotypes by evaluating endocrine-metabolic profile, inflammatory status, adherence to the MD, and body composition. The study population consisted of 94 treatment-naïve women with PCOS and obesity (BMI = 38.23 ± 6.62 kg/m2 and age = 24.12 ± 3.68 years). Compared PCOS MHO with PCOS MUO patients, the latter had higher levels of high-sensitivity C-reactive protein (hs-CRP) (p < 0.001), testosterone (p < 0.001), and insulin (p < 0.001), worse metabolic parameters, and higher Homeostatic Model Assessment of Insulin Resistance (HoMA-IR), Visceral Adiposity Index (VAI), and Fatty liver Index (FLI) (p < 0.001). Furthermore, PCOS MUO patients had lower adherence to the MD (p < 0.001) in spite of the same total energy intake (p = 0.102) as compared to PCOS MHO. The presence of MUO was associated with highest hs-CRP levels (OR = 1.49, p < 0.001), more severe hyperandrogenism and cardio-metabolic indices (p < 0.001). On the contrary, being PCOS MUO was associated with lower adherence to the MD (OR = 0.28, p < 0.001), and smaller PhAs (OR = 0.04, p < 0.001). Using a regression linear analysis model PREDIMED score entered at the first step (p < 0.001), followed by VAI (p < 0.001), and FLI (p = 0.032) in this analysis. At ROC analysis, a PREDIMED score of ≤4 (p < 0.001, AUC 0.926) could serve as a threshold for a significantly increased risk of presence the MUO-PCOS phenotype. To the best of our knowledge, this is the first study that characterized MHO and MUO-PCOS women on the basis of their adherence to the MD, body composition, and cardio-metabolic indices, providing evidence of the usefulness of adjunctive diagnostic parameters to better differentiate the MHO/MHO phenotypes in this cohort of PCOS patients with obesity.
Association between Coffee Consumption/Physical Exercise and Gastric, Hepatic, Colon, Breast, Uterine Cervix, Lung, Thyroid, Prostate, and Bladder Cancer
Authors: Kim So Young , Yoo Dae Myoung , Min Chanyang , Choi Hyo Geun
Published: November 2021

Although the effects of coffee consumption and physical exercise on the risk of cancer have been suggested, their interactions have not been investigated. The present cross-sectional study aimed to investigate the correlation of coffee consumption and physical exercise with cancer. Participants ≥40 years old in the Korean Genome and Epidemiology Study 2004–2016 were included (n = 162,220). Histories of gastric cancer, hepatic cancer, colon cancer, breast cancer, uterine cervix cancer, lung cancer, thyroid cancer, prostate cancer, and bladder cancer were analyzed according to the coffee consumption groups using logistic regression models. The odds among individuals in the >60 cups/month coffee group were lower for gastric cancer (adjusted odds ratio (aOR) = 0.80 (95% confidence intervals = 0.65–0.98)), hepatic cancer (0.32 (0.18–0.58)), colon cancer (0.53 (0.39–0.72)), breast cancer (0.56 (0.45–0.70)), and thyroid cancer (0.71 (0.59–0.85)) than for individuals in the no coffee group. Physical exercise of ≥150 min/week was correlated with higher odds for gastric cancer (1.18 (1.03–1.36)), colon cancer (1.52 (1.26–1.83)), breast cancer (1.53 (1.35–1.74)), thyroid cancer (1.42 (1.27–1.59)), and prostate cancer (1.61 (1.13–2.28)) compared to no exercise. Coffee consumption and physical exercise showed an interaction in thyroid cancer (p = 0.002). Coffee consumption was related to a decreased risk of gastric cancer, hepatic cancer, colon cancer, breast cancer, and thyroid cancer in the adult population. Physical exercise was positively correlated with gastric cancer, colon cancer, breast cancer, thyroid cancer, and prostate cancer.
Exploring the Gender-Related Perceptions of Male Nursing Students in Clinical Placement in the Asian Context: A Qualitative Study
Authors: Yip Yuk-Chiu , Yip Ka-Huen , Tsui Wai-King
Published: November 2021

The lack of gender diversity in the nursing profession has long been recognized as a cause for concern. Female nurses in many practice settings across nations continue to outnumber their male counterparts. Male nursing students may have perceived differences in the way they are treated in clinical practice; however, limited studies have been conducted to determine the unique perspectives of these students in terms of gender bias in their clinical learning. To address this knowledge gap, this study employed a qualitative descriptive approach to explore the experiences of male nursing students in clinical learning in an Asian context. Specifically, we examined the insights emerging from the thoughts and feelings of male nursing students from interactions with their clinical mentors, patients, and peers in their encounters during their clinical placement. From November 2019 to July 2020, data were collected through semi-structured interviews from 22 male participants aged 21–30 years (mean age: 22.7), enrolled in a prelicensure Bachelor of Nursing (honors) program in Hong Kong. Four themes emerged after a qualitative content analysis: (1) while the School of Nursing welcomes men, clinical settings are another story due to pragmatic considerations; (2) you are welcomed because you may be seen as a helper with greater physical strength; (3) male nursing students feel alienated in the obstetrics and gynecology practicum; (4) more male role models are desired in the clinical setting. Our findings indicate the need to promote gender awareness among faculty and clinical mentors, understand the factors hindering and facilitating the clinical practicum for male nursing students, improve the male nursing experience during the maternity practicum, and ensure access to more male role models to boost role socialization and learning.
Azimuthal Correlations of D Mesons with Charged Particles in Simulations with the ALICE Experiment
Authors: Frajna Eszter , Vertesi Robert
Published: November 2021

In this work, we present the results of a component-level analysis with Monte Carlo simulations, which aid the interpretation of recent ALICE results of the azimutal correlation distribution of prompt D mesons with charged hadrons in pp and p–Pb collisions at sNN = 5.02 TeV. Parton-level contributions and fragmentation properties are evaluated. Charm and beauty contributions are compared in order to identify the observables that serve as sensitive probes of the production and hadronisation of heavy quarks.
Celiac Disease in Conjunction with Hereditary Fructose Intolerance as a Rare Cause of Liver Steatosis with Mild Hypertransaminasemia—A Case Report
Authors: Bobrus-Chociej Anna , Pollak Agnieszka , Kopiczko Natalia , Flisiak-Jackiewicz Marta , Płoski Rafał , Lebensztejn Dariusz M.
Published: November 2021

Celiac disease (CD) has been associated with several genetic and autoimmune disorders, but its association with hereditary fructose intolerance (HFI) is very rare. The possibility of an association between CD and HFI should be considered, especially in patients with a lack of improvement after a gluten-free diet. Children with HFI often present with a wide range of symptoms, however, data about a strong aversion to fruits and sweets may be helpful to establish the diagnosis. The diagnosis of HFI should be confirmed in genetic testing. Both CD and HFI may present with liver steatosis with hypertransaminasemia. In patients with these two disorders, the dietary restrictions of gluten and fructose improve clinical symptoms and protect them from secondary complications. We report the case of a child with the concurrence of these two disorders.
Reversing an Oncogenic Epithelial-to-Mesenchymal Transition Program in Breast Cancer Reveals Actionable Immune Suppressive Pathways
Authors: Williams Michelle M. , Hafeez Sabrina A. , Christenson Jessica L. , O’Neill Kathleen I. , Hammond Nia G. , Richer Jennifer K.
Published: November 2021

Approval of checkpoint inhibitors for treatment of metastatic triple negative breast cancer (mTNBC) has opened the door for the use of immunotherapies against this disease. However, not all patients with mTNBC respond to current immunotherapy approaches such as checkpoint inhibitors. Recent evidence demonstrates that TNBC metastases are more immune suppressed than primary tumors, suggesting that combination or additional immunotherapy strategies may be required to activate an anti-tumor immune attack at metastatic sites. To identify other immune suppressive mechanisms utilized by mTNBC, our group and others manipulated oncogenic epithelial-to-mesenchymal transition (EMT) programs in TNBC models to reveal differences between this breast cancer subtype and its more epithelial counterpart. This review will discuss how EMT modulation revealed several mechanisms, including tumor cell metabolism, cytokine milieu and secretion of additional immune modulators, by which mTNBC cells may suppress both the innate and adaptive anti-tumor immune responses. Many of these pathways/proteins are under preclinical or clinical investigation as therapeutic targets in mTNBC and other advanced cancers to enhance their response to chemotherapy and/or checkpoint inhibitors.
Comparative Assessment of In Vitro and In Silico Methods for Aerodynamic Characterization of Powders for Inhalation
Authors: Ignjatović Jelisaveta , Šušteršič Tijana , Bodić Aleksandar , Cvijić Sandra , Đuriš Jelena , Rossi Alessandra , Dobričić Vladimir , Ibrić Svetlana , Filipović Nenad
Published: November 2021

In vitro assessment of dry powders for inhalation (DPIs) aerodynamic performance is an inevitable test in DPI development. However, contemporary trends in drug development also implicate the use of in silico methods, e.g., computational fluid dynamics (CFD) coupled with discrete phase modeling (DPM). The aim of this study was to compare the designed CFD-DPM outcomes with the results of three in vitro methods for aerodynamic assessment of solid lipid microparticle DPIs. The model was able to simulate particle-to-wall sticking and estimate fractions of particles that stick or bounce off the inhaler’s wall; however, we observed notable differences between the in silico and in vitro results. The predicted emitted fractions (EFs) were comparable to the in vitro determined EFs, whereas the predicted fine particle fractions (FPFs) were generally lower than the corresponding in vitro values. In addition, CFD-DPM predicted higher mass median aerodynamic diameter (MMAD) in comparison to the in vitro values. The outcomes of different in vitro methods also diverged, implying that these methods are not interchangeable. Overall, our results support the utility of CFD-DPM in the DPI development, but highlight the need for additional improvements in these models to capture all the key processes influencing aerodynamic performance of specific DPIs.
Chronobiology and Chronotherapy in Inflammatory Joint Diseases
Authors: Ursini Francesco , De Giorgi Alfredo , D’Onghia Martina , De Giorgio Roberto , Fabbian Fabio , Manfredini Roberto
Published: November 2021

Circadian rhythm perturbations can impact the evolution of different conditions, including autoimmune diseases. This narrative review summarizes the current understanding of circadian biology in inflammatory joint diseases and discusses the potential application of chronotherapy. Proinflammatory cytokines are key players in the development and progression of rheumatoid arthritis (RA), regulating cell survival/apoptosis, differentiation, and proliferation. The production and secretion of inflammatory cytokines show a dependence on the human day–night cycle, resulting in changing cytokine plasma levels over 24 h. Moreover, beyond the circadian rhythm of cytokine secretion, disturbances in timekeeping mechanisms have been proposed in RA. Taking into consideration chronotherapy concepts, modified-release (MR) prednisone tablets have been introduced to counteract the negative effects of night-time peaks of proinflammatory cytokines. Low-dose MR prednisone seems to be able to improve the course of RA, reduce morning stiffness and morning serum levels of IL-6, and induce significant clinical benefits. Additionally, methotrexate (MTX) chronotherapy has been reported to be associated with a significant improvement in RA activity score. Similar effects have been described for polymyalgia rheumatica and gout, although the available literature is still limited. Growing knowledge of chronobiology applied to inflammatory joint diseases could stimulate the development of new drug strategies to treat patients in accordance with biological rhythms and minimize side effects.
Observations on the Changing Shape of the Ice Mass and the Determination of the Sublimation End Point in Freeze-Drying: An Application for Through-Vial Impedance Spectroscopy (TVIS)
Authors: Pandya Bhaskar , Smith Geoff , Ermolina Irina , Polygalov Evgeny
Published: November 2021

Models for ice sublimation from a freeze-drying vial rely on the assumption of a planar ice interface up to ~25% loss of ice mass (which is difficult to qualify) whereas single-vial determinations of the sublimation endpoint (by temperature sensors) are based on the point when the observed temperature reaches a plateau, which cannot differentiate between sublimation and desorption-drying. In this work, the real part capacitance of TVIS vial(s) containing frozen water (during sublimation drying) was measured at 100 kHz. This parameter C′(100 kHz) was shown to be highly sensitive to the shape and volume of the ice mass and is therefore a useful parameter for monitoring ice sublimation. By placing a digital camera in front of an isolated TVIS vial containing ice, it was possible to relate the changes in the shape of the ice mass with the changes in the trajectory of the time profile of C′(100 kHz) and determine the point of deviation from a planar ice interface and ultimately determine the point when the last vestiges of ice disappear. Thereafter, the same characteristics of the C′(100 kHz) time-profile were identified for those TVIS vials located out of sight of the camera in a separate full-shelf lyo study, thereby obviating the need for photographic examination.
Miscibility and Solubility of Caffeine and Theophylline in Hydroxypropyl Methylcellulose
Authors: Leyk Edyta , Wesolowski Marek
Published: November 2021

As amorphization may improve the solubility and bioavailability of a drug substance, the aim of this work was to assess to what extent the crystallinity of caffeine (CAF) and theophylline (TF) can be reduced by homogenization with a polymeric excipient. To realize this purpose, the physical mixtures of both methylxanthines with hydroxypropyl methylcellulose (HPMC) were examined using differential scanning calorimetry (DSC), hot-stage microscopy (HSM), Fourier-transform infrared (FTIR) and Raman spectroscopy. Moreover, phase diagrams for the physical mixtures were calculated using theoretical data. Results of DSC experiments suggested that both CAF and TF underwent amorphization, which indicated proportional loss of crystallinity for methylxanthines in the mixtures with HPMC. Additionally, HSM revealed that no other crystalline or amorphous phases were created other than those observed for CAF and TF. FTIR and Raman spectra displayed all the bands characteristic for methylxanthines in mixtures with HPMC, thereby excluding changes in their chemical structures. However, changes to the intensity of the bands created by hydrogen bonds imply the formation of hydrogen bonding in the carbonyl group of methylxanthines and the methyl polymer group. This is consistent with data obtained using principal component analysis. The findings of these studies revealed the quantities of methylxanthines which may be dissolved in the polymer at a given temperature and the composition at which methylxanthines and polymer are sufficiently miscible to form a solid solution.
Current Advances in Lipid and Polymeric Antimicrobial Peptide Delivery Systems and Coatings for the Prevention and Treatment of Bacterial Infections
Authors: van Gent Miriam E. , Ali Muhanad , Nibbering Peter H. , Kłodzińska Sylvia N.
Published: November 2021

Bacterial infections constitute a threat to public health as antibiotics are becoming less effective due to the emergence of antimicrobial resistant strains and biofilm and persister formation. Antimicrobial peptides (AMPs) are considered excellent alternatives to antibiotics; however, they suffer from limitations related to their peptidic nature and possible toxicity. The present review critically evaluates the chemical characteristics and antibacterial effects of lipid and polymeric AMP delivery systems and coatings that offer the promise of enhancing the efficacy of AMPs, reducing their limitations and prolonging their half-life. Unfortunately, the antibacterial activities of these systems and coatings have mainly been evaluated in vitro against planktonic bacteria in less biologically relevant conditions, with only some studies focusing on the antibiofilm activities of the formulated AMPs and on the antibacterial effects in animal models. Further improvements of lipid and polymeric AMP delivery systems and coatings may involve the functionalization of these systems to better target the infections and an analysis of the antibacterial activities in biologically relevant environments. Based on the available data we proposed which polymeric AMP delivery system or coatings could be profitable for the treatment of the different hard-to-treat infections, such as bloodstream infections and catheter- or implant-related infections.
Immunomodulatory Nanoparticles Mitigate Macrophage Inflammation via Inhibition of PAMP Interactions and Lactate-Mediated Functional Reprogramming of NF-κB and p38 MAPK
Authors: Lasola Jackline Joy Martín , Cottingham Andrea L. , Scotland Brianna L. , Truong Nhu , Hong Charles C. , Shapiro Paul , Pearson Ryan M.
Published: November 2021

Inflammation is a key homeostatic process involved in the body’s response to a multitude of disease states including infection, autoimmune disorders, cancer, and other chronic conditions. When the initiating event is poorly controlled, severe inflammation and globally dysregulated immune responses can occur. To address the lack of therapies that efficaciously address the multiple aspects of the dysregulated immune response, we developed cargo-less immunomodulatory nanoparticles (iNPs) comprised of poly(lactic acid) (PLA) with either poly(vinyl alcohol) (PVA) or poly(ethylene-alt-maleic acid) (PEMA) as stabilizing surfactants and investigated the mechanisms by which they exert their inherent anti-inflammatory effects. We identified that iNPs leverage a multimodal mechanism of action by physically interfering with the interactions between pathogen-associated molecular patterns (PAMPs) and bone marrow-derived macrophages (BMMΦs). Additionally, we showed that iNPs mitigate proinflammatory cytokine secretions induced by LPS via a time- and composition-dependent abrogation of NF-κB p65 and p38 MAPK activation. Lastly, inhibition studies were performed to establish the role of a pH-sensing G-protein-coupled receptor, GPR68, on contributing to the activity of iNPs. These data provide evidence for the multimodal mechanism of action of iNPs and establish their potential use as a novel therapeutic for the treatment of severe inflammation.
Despair as a Threat to Meaning: Kierkegaard’s Challenge to Objectivist Theories
Authors: Hanson Jeffrey
Published: November 2021

The question of meaning in life has enjoyed renewed attention in analytic discourse over the last few decades. Despite the apparently “existential” quality of this topic, existential philosophy has had little impact on this re-energized conversation. This paper draws on Kierkegaard’s The Sickness unto Death in order to challenge the objectivist theory of meaning in life. According to that theory, a meaningful life is one replete with objective goods. Kierkegaard, however, exposits four forms of the spiritual sickness he calls despair that are compatible with the possession of objective goods. If this account is convincing, it poses a challenge to the objectivist view, suggesting that a subjective contribution is also necessary to fully account for meaning in life. By a process of negative inference, this paper concludes by sketching out what this subjective contribution might look like and suggests the term “authenticity” in order to capture this subjective element of a meaningful life.
Transition-Metal-Free Access to 2-Subsituted Indolines from Indoles via Dearomative Nucleophilic Addition Using Two-Molecule Organic Photoredox Catalysts
Authors: Tanaka Yosuke , Ikeda Takumi , Nachi Yasuhiro , Mizuno Taisei , Maeda Kousuke , Sakamoto Chisato , Yamawaki Mugen , Morita Toshio , Yoshimi Yasuharu
Published: November 2021

A Photoinduced dearomative nucleophilic addition to N-Boc indoles mediated by two-molecule organic photoredox catalysts such as phenanthrene and 1,4-dicyanobenzene with UV irradiation furnished 2-substituted indolines in moderate to quantitative yields. Hydroxide, alkoxide, and cyanide ions can be used as a nucleophile to provide 2-hydroxy, 2-alkoxy, and 2-cyanoindolines, respectively. Both electron-rich and -deficient indoles, including tryptophan derivatives, can be employed in the photoreaction to provide various indolines. This method provides transition-metal-free access to 2-subsituted indolines from indoles using organic photoredox catalysts under mild conditions.
Mueller Matrix Polarimetry with Invariant Polarization Pattern Beams
Authors: de Sande Juan Carlos González , Piquero Gemma , Suárez-Bermejo Juan Carlos , Santarsiero Massimo
Published: November 2021

A wide class of nonuniformly totally polarized beams that preserve their transverse polarization pattern during paraxial propagation was studied. Beams of this type are of interest, in particular, in polarimetric techniques that use a single input beam for the determination of the Mueller matrix of a homogeneous sample. In these cases, in fact, it is possible to test the sample response to several polarization states at once. The propagation invariance of the transverse polarization pattern is an interesting feature for beams used in these techniques, because the polarization state of the output beam can be detected at any transverse plane after the sample, without the use of any imaging/magnifying optical system. Furthermore, exploiting the great variety of the beams of this class, the ones that better fit specific experimental constrains can be chosen. In particular, the class also includes beams that present all possible polarization states across their transverse section (the full Poincaré beams (FPB)). The use of the latter has recently been proposed to increase the accuracy of the recovered Mueller matrix elements. Examples of FPBs with propagation-invariant polarization profiles and its use in polarimetry are discussed in detail. The requirement of invariance of the polarization pattern can be limited to the propagation in the far field. In such a case, less restrictive conditions are derived, and a wider class of beams is found.