bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of the convective heat transfer during full melt off of ice slurry in laminar and non-Newtonian flows
Authors: Mellari Souheila
Published: August 2022

This paper presents the experimental results of the heat transfer coefficient of ice slurry in the distribution phase in order to conserve fish and shellfish where the ideal temperature oscillates between 0 °C and 2 °C. The required energy power absorbed during the exchange between the ice slurry and the air is assimilated to the transport capacity (in kW of cooling at full melt off). The secondary fluid was prepared by mixing monopropylene glycol, MPG, and water to obtain an initial concentration varying from 5 to 24% (the initially freezing temperature varying from − 1.84 °C to – 9.39 °C). The heat transfer coefficients for ice slurry in laminar flow conditions increased with velocity and ice concentration but decreased with increasing MPG concentration, according to the results. The heat transfer coefficients for ice slurry in laminar flow conditions increased with velocity and ice concentration but decreased with increasing MPG concentration, according to the results. The heat transfer coefficients of ice slurry are of the order of 7000 W.m−2.K−1.Moreover, the rheological behavior of the ice slurry greatly influenced the results under certain conditions. A new average Nusselt correlation was proposed that accurately predicts the superficial heat transfer coefficients of laminar flow of ice slurry through the horizontal pipe.
MicroRNA–Disease Network Analysis Repurposes Methotrexate for the Treatment of Abdominal Aortic Aneurysm in Mice
Authors: Shen Yicong , Gao Yuanxu , Shi Jiangcheng , Huang Zhou , Dai Rongbo , Fu Yi , Zhou Yuan , Kong Wei , Cui Qinghua
Published: August 2022

Abdominal aortic aneurysm (AAA) is a permanent dilatation of the abdominal aorta and is highly lethal. The main purpose of the current study is to search for noninvasive medical therapies for AAA, for which there is currently no effective drug therapy. Network medicine represents a cutting-edge technology, as analysis and modeling of disease networks can provide critical clues regarding the etiology of specific diseases and therapeutics that may be effective. Here, we proposed a novel algorithm to quantify disease relations based on a large accumulated microRNA–disease association dataset and then built a disease network covering 15 disease classes and 304 diseases. Analysis revealed some patterns for these diseases. For instance, diseases tended to be clustered and coherent in the network. Surprisingly, we found that AAA showed the strongest similarity with rheumatoid arthritis and systemic lupus erythematosus, both of which are autoimmune diseases, suggesting that AAA could be one type of autoimmune diseases in etiology. Based on this observation, we further hypothesized that drugs for autoimmune diseases could be repurposed for the prevention and therapy of AAA. Finally, animal experiments confirmed that methotrexate, a drug for autoimmune diseases, was able to alleviate the formation and development of AAA.
Electric Machines
Authors: Aliprantis Dionysios , Wasynczuk Oleg
Published: August 2022

Offering a new perspective, this textbook demystifies the operation of electric machines by providing an integrated understanding of electromagnetic fields, electric circuits, numerical analysis, and computer programming. It presents fundamental concepts in a rigorous manner, emphasising underlying physical modelling assumptions and limitations, and provides detailed explanations of how to implement the finite element method to explore these concepts using Python. It includes explanations of the conversion of concepts into algorithms, and algorithms into code, and examples building in complexity, from simple linear-motion electromagnets to rotating machines. Over 100 theoretical and computational end-of-chapter exercises test understanding, with solutions for instructors and downloadable Python code available online.
Ideal for graduates and senior undergraduates studying electric machines, electric machine design and control, and power electronic converters and power systems engineering, this textbook is also a solid reference for engineers interested in understanding, analysing and designing electric motors, generators, and transformers.
Crisis as driver of digital transformation? Scottish local governments’ response to COVID-19
Authors: Gangneux Justine , Joss Simon
Published: August 2022

The response to the COVID-19 pandemic has, from the outset, been characterized by a strong focus on real-time data intelligence and the use of data-driven technologies. Against this backdrop, this article investigates the impacts of the pandemic on Scottish local government’s data practices and, in turn, whether the crisis acted as a driver for digital transformation. Mobilizing the literatures on digital government transformation, and on the impacts of crises on public administrations, the article provides insights into the dynamics of digital transformation during a heightened period of acute demands on the public sector. The research evidences an intensification of public sector data use and sharing in Scottish local authorities, with focus on health-related data and the integration of existing datasets to gather local intelligence. The research reveals significant changes related to the technical and social systems of local government organizations. These include the repurposing and adoption of information systems, the acceleration of inter and intraorganizational data sharing processes, as well as changes in ways of working and in attitudes toward data sharing and collaborations. Drawing on these findings, the article highlights the importance of identifying and articulating specific data needs in relation to concrete policy questions in order to render digital transformation relevant and effective. The article also points to the need of addressing the persistent systemic challenges underlying public sector data engagement through, on one hand, sustained investment in data capabilities and infrastructures and, on the other, support for cross-organizational collaborative spaces and networks.
Subharmonic parametric instability in nearly brimful circular cylinders: a weakly nonlinear analysis
Authors: Bongarzone Alessandro , Viola Francesco , Camarri Simone , Gallaire François
Published: August 2022

In labscale Faraday experiments, meniscus waves respond harmonically to small-amplitude forcing without threshold, hence potentially cloaking the instability onset of parametric waves. Their suppression can be achieved by imposing a contact line pinned at the container brim with static contact angle

$\theta _s=90^{\circ }$

(brimful condition). However, tunable meniscus waves are desired in some applications as those of liquid-based biosensors, where they can be controlled adjusting the shape of the static meniscus by slightly underfilling/overfilling the vessel (

$\theta _s\ne 90^{\circ }$

) while keeping the contact line fixed at the brim. Here, we refer to this wetting condition as nearly brimful. Although classic inviscid theories based on Floquet analysis have been reformulated for the case of a pinned contact line (Kidambi, J. Fluid Mech., vol. 724, 2013, pp. 671–694), accounting for (i) viscous dissipation and (ii) static contact angle effects, including meniscus waves, makes such analyses practically intractable and a comprehensive theoretical framework is still lacking. Aiming at filling this gap, in this work we formalize a weakly nonlinear analysis via multiple time scale method capable of predicting the impact of (i) and (ii) on the instability onset of viscous subharmonic standing waves in both brimful and nearly brimful circular cylinders. Notwithstanding that the form of the resulting amplitude equation is in fact analogous to that obtained by symmetry arguments (Douady, J. Fluid Mech., vol. 221, 1990, pp. 383–409), the normal form coefficients are here computed numerically from first principles, thus allowing us to rationalize and systematically quantify the modifications on the Faraday tongues and on the associated bifurcation diagrams induced by the interaction of meniscus and subharmonic parametric waves.
Light absorption and albedo reduction by pigmented microalgae on snow and ice
Authors: Chevrollier Lou-Anne , Cook Joseph M. , Halbach Laura , Jakobsen Hans , Benning Liane G. , Anesio Alexandre M. , Tranter Martyn
Published: August 2022

Pigmented microalgae inhabiting snow and ice environments lower the albedo of glacier and ice-sheet surfaces, significantly enhancing surface melt. Our ability to accurately predict their role in glacier and ice-sheet surface mass balance is limited by the current lack of empirical data to constrain their representation in predictive models. Here we present new empirical optical properties for snow and ice algae and incorporate them in a radiative transfer model to investigate their impact on snow and ice surface albedo. We found ice algal cells to be more efficient absorbers than snow algal cells, but their blooms had comparable impact on surface albedo due to the different photic conditions of their habitats. We then used the model to reconstruct the effect of ice algae on bare ice albedo spectra collected at our field site in southern Greenland, where blooms dropped the albedo locally by between 3 and 43%, equivalent to 1–10 L m$^{-2}$ d$^{-1}$ of melted ice. Using the newly parametrized model, future studies could investigate biological albedo reduction and algal quantification from remote hyperspectral and multispectral imagery.
On environmental justice, Part I: an intuitive conservation dilemma
Authors: Mazor Joseph
Published: August 2022

This article introduces an intuitive conservation dilemma called the Canyon Dilemma: Is it possible to condemn the mining of the Grand Canyon, even by a poor generation, while also permitting this generation’s mining of an unremarkable small canyon? It then argues that not one of several prominent theories of environmental justice, including various forms of egalitarianism, welfarism, deep-ecological theories, communitarianism and free-market environmentalism, can navigate this dilemma. The article concludes by highlighting the dilemma-navigating potential of the equal-claims idea – the idea that the natural world is something to which every human being, present and future, has an equal, substantive claim.
Petrostructural and Geochronological Constraints on Devonian Extension‐Shortening Cycle in the Chinese Altai: Implications for Retreating‐Advancing Subduction
Authors: Kong Lingzhu , Jiang Yingde , Schulmann Karel , Zhang Jian , Weinberg Roberto F. , Sun Min , Wang Sheng , Shu Tan , Ning Jun
Published: August 2022

Structural and metamorphic effects of a Devonian extension‐shortening cycle in the NW Chinese Altai were investigated using combined structural‐petrological analysis and zircon and monazite U‐Pb geochronology. Structural observations revealed a ubiquitous, sub‐horizontal metamorphic fabric (S1), which was originally sub‐parallel with horizontal bedding (S0) of supracrustal Devonian volcanic‐sedimentary basins. This metamorphic fabric is defined by a sillimanite‐bearing migmatitic gneissosity associated with extensional shear bands and a cordierite‐bearing schistosity in the orogenic lower and middle crust, respectively. Staurolite relics preserved in the S1 fabric are interpreted as relics of older, higher pressure Barrovian‐type metamorphism. These structural‐metamorphic features are correlated with decompression and heating and interpreted as reflecting a crustal‐scale extensional phase. S1 was folded to form N‐S‐oriented upright antiforms cored by migmatites and locally transposed by steep S2 foliation during a ∼W‐E‐directed shortening (in current coordinates). Andalusite and cordierite overgrew the migmatitic S2 foliation attesting to decreasing pressure associated with vertical extrusion of migmatites in cores of F2 antiforms. New U‐Pb age data from subhorizontally foliated migmatites suggest that the D1 extension started at least before ∼410 Ma. Age data of syn‐D2 granite intrusions and dykes constrain the D2 shortening starting soon after D1 extension and ending at ∼378 Ma. Combined with regional data, cycles of extensional and contractional tectonic regimes can be defined, which also show eastward migration along‐strike of the Altai range. Such extension‐shortening cycles could result from alternating retreating and advancing subduction, which governed the evolution of the Altai accretionary system from the Ordovician until the Carboniferous.
Climate Change Has Already Aggravated 58% of Infectious Diseases
Authors: Duncombe Jenessa
Published: August 2022

Global warming has, in certain instances, amped up some of the world’s most deadly diseases.
Reconciling and Improving Formulations for Thermodynamics and Conservation Principles in Earth System Models (ESMs)
Authors: Lauritzen P. H. , Kevlahan N. K.‐R. , Toniazzo T. , Eldred C. , Dubos T. , Gassmann A. , Larson V. E. , Jablonowski C. , Guba O. , Shipway B. , Harrop B. E. , Lemarié F. , Tailleux R. , Herrington A. R. , Large W. , Rasch P. J. , Donahue A. S. , Wan H. , Conley A. , Bacmeister J. T.
Published: August 2022

This paper provides a comprehensive derivation of the total energy equations for the atmospheric components of Earth System Models (ESMs). The assumptions and approximations made in this derivation are motivated and discussed. In particular, it is emphasized that closing the energy budget is conceptually challenging and hard to achieve in practice without resorting to ad hoc fixers. As a concrete example, the energy budget terms are diagnosed in a realistic climate simulation using a global atmosphere model. The largest total energy errors in this example are spurious dynamical core energy dissipation, thermodynamic inconsistencies (e.g., coupling parameterizations with the host model) and missing processes/terms associated with falling precipitation and evaporation (e.g., enthalpy flux between components). The latter two errors are not, in general, reduced by increasing horizontal resolution. They are due to incomplete thermodynamic and dynamic formulations. Future research directions are proposed to reconcile and improve thermodynamics formulations and conservation principles.
The role of genetic predisposition in cardiovascular risk after cancer diagnosis: a matched cohort study of the UK Biobank
Authors: Yang Huazhen , Zeng Yu , Chen Wenwen , Sun Yajing , Hu Yao , Ying Zhiye , Wang Junren , Qu Yuanyuan , Fang Fang , Valdimarsdóttir Unnur A. , Song Huan
Published: August 2022

Background

Evidence is scarce regarding the potential modifying role of disease susceptibility on the association between a prior cancer diagnosis and cardiovascular disease (CVD).

Methods

We conducted a matched cohort study of UK Biobank including 78,860 individuals with a cancer diagnosis between January 1997 and January 2020, and 394,300 birth year and sex individually matched unexposed individuals. We used Cox model to assess the subsequent relative risk of CVD, which was further stratified by individual genetic predisposition.

Results

During nearly 23 years of follow-up, an elevated risk of CVD was constantly observed among cancer patients, compared to their matched unexposed individuals. Such excess risk was most pronounced (hazard ratio [HR] = 5.28, 95% confidence interval [CI] 4.90–5.69) within 3 months after a cancer diagnosis, which then decreased rapidly and stabilised for >6 months (HR = 1.22, 95% CI 1.19–1.24). For all the studied time periods, stratification analyses by both levels of polygenic risk score for CVD and by family history of CVD revealed higher estimates among individuals with lower genetic risk predisposition.

Conclusions

Our findings suggest that patients with a recent cancer diagnosis were at an increased risk of multiple types of CVD and the excess CVD risk was higher among individuals with lower genetic susceptibility to CVD, highlighting a general need for enhanced psychological assistance and clinical surveillance of CVD among newly diagnosed cancer patients.
How do women who are informed that they are at increased risk of breast cancer appraise their risk? A systematic review of qualitative research
Authors: Woof Victoria G. , Howell Anthony , McWilliams Lorna , Gareth Evans D. , French David P.
Published: August 2022

This review aimed to synthesise qualitative research on how women notified that they are at increased risk of breast cancer view their risk. Five electronic databases were systematically reviewed for qualitative research investigating how women who have received an increased breast cancer risk estimate appraise their risk status. Fourteen records reporting 12 studies were included and critically appraised. Data were thematically synthesised.Four analytical themes were generated. Women appraise their risk of breast cancer through comparison with their risk of other familial diseases. Clinically derived risk estimates were understood in relation to pre-conceived risk appraisals, with incongruences met with surprise. Family history is relied upon strongly, with women exploring similarities and differences in attributes between themselves and affected relatives to gauge the likelihood of diagnosis. Women at increased risk reported living under a cloud of inevitability or uncertainty regarding diagnosis, resulting in concerns about risk management. Women hold stable appraisals of their breast cancer risk which appear to be mainly formed through their experiences of breast cancer in the family. Healthcare professionals should explore women’s personal risk appraisals prior to providing clinically derived risk estimates in order to address misconceptions, reduce concerns about inevitability and increase perceived control over risk reduction.
A neural correlate of perceptual segmentation in macaque middle temporal cortical area
Authors: Clark Andrew M. , Bradley David C.
Published: August 2022

High-resolution vision requires fine retinal sampling followed by integration to recover object properties. Importantly, accuracy is lost if local samples from different objects are intermixed. Thus, segmentation, grouping of image regions for separate processing, is crucial for perception. Previous work has used bi-stable plaid patterns, which can be perceived as either a single or multiple moving surfaces, to study this process. Here, we report a relationship between activity in a mid-level site in the primate visual pathways and segmentation judgments. Specifically, we find that direction selective middle temporal neurons are sensitive to texturing cues used to bias the perception of bi-stable plaids and exhibit a significant trial-by-trial correlation with subjective perception of a constant stimulus. This correlation is greater in units that signal global motion in patterns with multiple local orientations. Thus, we conclude the middle temporal area contains a signal for segmenting complex scenes into constituent objects and surfaces.
Flexible iontronics based on 2D nanofluidic material
Authors: Wei Di , Yang Feiyao , Jiang Zhuoheng , Wang Zhonglin
Published: August 2022

Iontronics focuses on the interactions between electrons and ions, playing essential roles in most processes across physics, chemistry and life science. Osmotic power source as an example of iontronics, could transform ion gradient into electrical energy, however, it generates low power, sensitive to humidity and can’t operate under freezing point. Herein, based on 2D nanofluidic graphene oxide material, we demonstrate an ultrathin (∼10 µm) osmotic power source with voltage of 1.5 V, volumetric specific energy density of 6 mWh cm−3 and power density of 28 mW cm−3, achieving the highest values so far. Coupled with triboelectric nanogenerator, it could form a self-charged conformable triboiontronic device. Furthermore, the 3D aerogel scales up areal power density up to 1.3 mW cm−2 purely from ion gradient based on nanoconfined enhancement from graphene oxide that can operate under −40 °C and overcome humidity limitations, enabling to power the future implantable electronics in human-machine interface.
Investigating disparity in access to Australian clinical genetic health services for Aboriginal and Torres Strait Islander people
Authors: Luke Joanne , Dalach Philippa , Tuer Lindsay , Savarirayan Ravi , Ferdinand Angeline , McGaughran Julie , Kowal Emma , Massey Libby , Garvey Gail , Dawkins Hugh , Jenkins Misty , Paradies Yin , Pearson Glenn , Stutterd Chloe A. , Baynam Gareth , Kelaher Margaret
Published: August 2022

Globally, there is a recognised need that all populations should be able to access the benefits of genomics and precision medicine. However, achieving this remains constrained by a paucity of data that quantifies access to clinical genomics, particularly amongst Indigenous populations. Using administrative data from clinical genetic health services across three Australian jurisdictions (states/territories), we investigate disparities in the scheduling and attendance of appointments among Aboriginal and/or Torres Strait Islander people, compared to non-Indigenous people. For 14,870 appointments scheduled between 2014–2018, adjusted Multivariate Poisson Regression models revealed that Aboriginal and/or Torres Strait Islander people were scheduled fewer appointments (IRR 0.73 [0.68–0.80], <0.001) and attended at lower rates (IRR 0.85 [0.78–0.93], <0.001). Within this population, adults, females, remote residents, and those presenting in relation to cancer or prenatal indications experienced the greatest disparity in access. These results provide important baseline data related to disparities in access to clinical genomics in Australia.
Self-assembly as a tool to study microscale curvature and strain-dependent magnetic properties
Authors: Singh Balram , Otálora Jorge. A. , Kang Tong H. , Soldatov Ivan , Karnaushenko Dmitriy D. , Becker Christian , Schäfer Rudolf , Karnaushenko Daniil , Neu Volker , Schmidt Oliver G.
Published: August 2022

The extension of 2D ferromagnetic structures into 3D curved geometry enables to tune its magnetic properties such as uniaxial magnetic anisotropy. Tuning the anisotropy with strain and curvature has become a promising ingredient in modern electronics, such as flexible and stretchable magnetoelectronic devices, impedance-based field sensors, and strain gauges, however, has been limited to extended thin films and to only moderate bending. By applying a self-assembly rolling technique using a polymeric platform, we provide a template that allows homogeneous and controlled bending of a functional layer adhered to it, irrespective of its shape and size. This is an intriguing possibility to tailor the sign and magnitude of the surface strain of integrated, micron-sized devices. In this article, the impact of strain and curvature on the magnetic ground state and anisotropy is quantified for thin-film Permalloy micro-scale structures, fabricated on the surface of the tubular architectures, using solely electrical measurements.
A novel hybrid model for six main pollutant concentrations forecasting based on improved LSTM neural networks
Authors: Xu Shenyi , Li Wei , Zhu Yuhan , Xu Aiting
Published: August 2022

In recent years, air pollution has become a factor that cannot be ignored, affecting human lives and health. The distribution of high-density populations and high-intensity development and construction have accentuated the problem of air pollution in China. To accelerate air pollution control and effectively improve environmental air quality, the target of our research was cities with serious air pollution problems to establish a model for air pollution prediction. We used the daily monitoring data of air pollution from January 2016 to December 2020 for the respective cities. We used the long short term memory networks (LSTM) algorithm model to solve the problem of gradient explosion in recurrent neural networks, then used the particle swarm optimization algorithm to determine the parameters of the CNN-LSTM model, and finally introduced the complete ensemble empirical mode decomposition of adaptive noise (CEEMDAN) decomposition to decompose air pollution and improve the accuracy of model prediction. The experimental results show that compared with a single LSTM model, the CEEMDAN-CNN-LSTM model has higher accuracy and lower prediction errors. The CEEMDAN-CNN-LSTM model enables a more precise prediction of air pollution, and may thus be useful for sustainable management and the control of air pollution.
Evaluating the performance of Bayesian and frequentist approaches for longitudinal modeling: application to Alzheimer’s disease
Authors: Pérez-Millan Agnès , Contador José , Tudela Raúl , Niñerola-Baizán Aida , Setoain Xavier , Lladó Albert , Sánchez-Valle Raquel , Sala-Llonch Roser
Published: August 2022

Linear mixed effects (LME) modelling under both frequentist and Bayesian frameworks can be used to study longitudinal trajectories. We studied the performance of both frameworks on different dataset configurations using hippocampal volumes from longitudinal MRI data across groups—healthy controls (HC), mild cognitive impairment (MCI) and Alzheimer’s disease (AD) patients, including subjects that converted from MCI to AD. We started from a big database of 1250 subjects from the Alzheimer’s disease neuroimaging initiative (ADNI), and we created different reduced datasets simulating real-life situations using a random-removal permutation-based approach. The number of subjects needed to differentiate groups and to detect conversion to AD was 147 and 115 respectively. The Bayesian approach allowed estimating the LME model even with very sparse databases, with high number of missing points, which was not possible with the frequentist approach. Our results indicate that the frequentist approach is computationally simpler, but it fails in modelling data with high number of missing values.
One-stop-shop CT arthrography of the wrist without subject repositioning by means of gantry-free cone-beam CT
Authors: Luetkens Karsten Sebastian , Grunz Jan-Peter , Paul Mila Marie , Huflage Henner , Conrads Nora , Patzer Theresa Sophie , Gruschwitz Philipp , Ergün Süleyman , Bley Thorsten Alexander , Kunz Andreas Steven
Published: August 2022

Modern cone-beam CT systems are capable of ultra-high-resolution 3D imaging in addition to conventional radiography and fluoroscopy. The combination of various imaging functions in a multi-use setup is particularly appealing for musculoskeletal interventions, such as CBCT arthrography (CBCTA). With this study, we aimed to investigate the feasibility of CBCTA of the wrist in a “one-stop-shop” approach with a gantry-free twin robotic scanner that does not require repositioning of subjects. Additionally, the image quality of CBCTA was compared to subsequent arthrograms on a high-end multidetector CT (MDCTA). Fourteen cadaveric wrists received CBCTA with four acquisition protocols. Specimens were then transferred to the CT suite for additional MDCTA. Dose indices ranged between 14.3 mGy (120 kVp/100 effective mAs; full-dose) and 1.0 mGy (70 kVp/41 effective mAs; ultra-low-dose) for MDCTA and between 17.4 mGy (80 kVp/2.5 mAs per pulse; full-dose) and 1.2 mGy (60 kVp/0.5 mAs per pulse; ultra-low-dose) for CBCTA. Subjective image quality assessment for bone, cartilage and ligamentous tissue was performed by seven radiologists. The interrater reliability was assessed by calculation of the intraclass correlation coefficient (ICC) based on a two-way random effects model. Overall image quality of most CBCTA was deemed suitable for diagnostic use in contrast to a considerable amount of non-diagnostic MDCTA examinations (38.8%). The depiction of bone, cartilage and ligaments in MDCTA with any form of dose reduction was inferior to any CBCTA scan with at least 0.6 mAs per pulse (all p < 0.001). Full-dose MDCTA and low-dose CBCTA were of equal quality for bone tissue visualization (p = 0.326), whereas CBCTA allowed for better depiction of ligaments and cartilage (both p < 0.001), despite merely one third of radiation exposure (MDCTA–14.3 mGy vs. CBCTA–4.5 mGy). Moderate to good interrater reliability was ascertained for the assessment all tissues (ICC 0.689–0.756). Overall median examination time for CBCTA was 5.4 min (4.8–7.2 min). This work demonstrates that substantial dose reduction can be achieved in CT arthrography of the wrist while maintaining diagnostic image quality by employing the cone-beam CT mode of a twin robotic X-ray system. The ability of the multi-use X-ray system to switch between fluoroscopy mode and 3D imaging allows for “one-stop-shop” CBCTA in minimal examination time without the need for repositioning.
Translational feasibility and efficacy of nasal photodynamic disinfection of SARS-CoV-2
Authors: Pires Layla , Wilson Brian C. , Bremner Rod , Lang Amanda , Larouche Jeremie , McDonald Ryan , Pearson Joel D. , Trcka Daniel , Wrana Jeff , Wu James , Whyne Cari M.
Published: August 2022

The lack of therapeutic options to fight Covid-19 has contributed to the current global pandemic. Despite the emergence of effective vaccines, development of broad-spectrum antiviral treatment remains a significant challenge, in which antimicrobial photodynamic therapy (aPDT) may play a role, especially at early stages of infection. aPDT of the nares with methylene blue (MB) and non-thermal light has been successfully utilized to inactivate both bacterial and viral pathogens in the perioperative setting. Here, we investigated the effect of MB-aPDT to inactivate human betacoronavirus OC43 and SARS-CoV-2 in vitro and in a proof-of-principle COVID-19 clinical trial to test, in a variety of settings, the practicality, technical feasibility, and short-term efficacy of the method. aPDT yielded inactivation of up to 6-Logs in vitro, as measured by RT-qPCR and infectivity assay. From a photo-physics perspective, the in vitro results suggest that the response is not dependent on the virus itself, motivating potential use of aPDT for local destruction of SARS-CoV-2 and its variants. In the clinical trial we observed variable effects on viral RNA in nasal-swab samples as assessed by RT-qPCR attributed to aPDT-induced RNA fragmentation causing falsely-elevated counts. However, the viral infectivity in clinical nares swabs was reduced in 90% of samples and undetectable in 70% of samples. This is the first demonstration based on quantitative clinical viral infectivity measurements that MB-aPDT is a safe, easily delivered and effective front-line technique that can reduce local SARS-CoV-2 viral load.
Performance of the three-dimensional laser scanning method to monitor the moisture content of similar material models
Authors: Zha Jianfeng , Yang Xicong , Li Huaizhan , Yang Mohan , Zhong Chongwu , Song Kun
Published: August 2022

In mining safety and other fields, similar material simulation is the main research method to study the movement and deformation of rock formation and ground surface. However, the inaccurate subsidence laws could be obtained because the strength of the composition materials like gypsum and lime is easily affected by moisture. Therefore, it is crucial to monitor the moisture content when carrying simulation experiments. This paper discussed the feasibility of indirectly measuring the moisture content of similar material models using the three-dimensional (3D) laser scanning reflection intensity through three experiments on similar material specimens. The results showed that the laser reflection intensity was sensitive to the moisture content, incidence angle, and distance with three different relationships and the influence of the two factors could be weakened through the established correction models. However, it was recommended restricting the incidence angle to less than 20° and setting the distance from 4 to 10 m to reduce the complexity of correction. The accuracy of this method reached 1.1% under the monitoring condition of 4 m and the normal incidence, which could meet the requirements for monitoring the moisture content of similar material models. The research results of the paper provide a new method to monitor the moisture content in similar material models.
Investigation of the long-term healing response of the liver to boiling histotripsy treatment in vivo
Authors: Heo Jeongmin , Joung Chanmin , Pahk Kisoo , Pahk Ki Joo
Published: August 2022

Boiling histotripsy (BH) is a promising High-Intensity Focused Ultrasound technique that can be employed to mechanically fractionate solid tumours. Whilst studies have shown the feasibility of BH to destroy liver cancer, no study has reported on the healing process of BH-treated liver tissue. We therefore extensively investigated the evolution of the healing response of liver to BH in order to provide an insight into the healing mechanisms. In the present study, 14 Sprague Dawley rats underwent the BH treatment and were sacrificed on days 0, 3, 7, 14, and 28 for morphological, histological, serological and qPCR analyses. The area of the treated region was 1.44 cm2 (1.2 cm × 1.2 cm). A well-defined BH lesion filled with coagulated blood formed on day 0. A week after the treatment, fibroblast activation was induced at the treatment site, leading to the formation of extracellular matrix structure (ECM). The ECM was then disrupted for 7 to 28 days. Regenerated normal hepatocytes and newly formed blood vessels were found within the BH region with the absence of hepatic fibrosis. No significant morphological, histological and genetic changes around the BH lesion occurred. These results suggest that BH could be a safe and promising therapeutic tool for treating solid tumours without inducing any significant adverse effect such as the formation of liver fibrosis.
Single-shot ptychography at a soft X-ray free-electron laser
Authors: Kharitonov Konstantin , Mehrjoo Masoud , Ruiz-Lopez Mabel , Keitel Barbara , Kreis Svea , Gang Seung-gi , Pan Rui , Marras Alessandro , Correa Jonathan , Wunderer Cornelia B. , Plönjes Elke
Published: August 2022

In this work, single-shot ptychography was adapted to the XUV range and, as a proof of concept, performed at the free-electron laser FLASH at DESY to obtain a high-resolution reconstruction of a test sample. Ptychography is a coherent diffraction imaging technique capable of imaging extended samples with diffraction-limited resolution. However, its scanning nature makes ptychography time-consuming and also prevents its application for mapping of dynamical processes. Single-shot ptychography can be realized by collecting the diffraction patterns of multiple overlapping beams in one shot and, in recent years, several concepts based on two con-focal lenses were employed in the visible regime. Unfortunately, this approach cannot be extended straightforwardly to X-ray wavelengths due to the use of refractive optics. Here, a novel single-shot ptychography setup utilizes a combination of X-ray focusing optics with a two-dimensional beam-splitting diffraction grating. It facilitates single-shot imaging of extended samples at X-ray wavelengths.
Inflammatory and proapoptotic effects of inhaling gasoline fumes on the lung and ameliorative effects of fenugreek seeds
Authors: Abdrabouh Abeer E.
Published: August 2022

Impacts of inhaling gasoline fumes on the lungs of adult male rats and the alleviating role of fenugreek seeds were evaluated. Twenty-four rats were divided into four groups, unexposed control and fenugreek groups, gasoline exposed groups for 6 h/6 day/week for 10 weeks with and without supplementation of fenugreek seed powder in food (5% w/w). Rats exposed to gasoline fumes showed significant elevation in lung tumor necrosis factor-α, as an inflammatory marker, and the proapoptotic marker Bax with a reduction in the antiapoptotic marker Bcl2. Moreover, remarkable elevations in transforming growth factor-β1, collagen and hydroxyproline were observed as fibrotic markers. Lung oxidative stress markers (hydrogen peroxides, malondialdehyde, and protein carbonyl) increased significantly along with marked decrease in total antioxidant capacity, superoxide dismutase, and catalase levels. Additionally, marked decreases in white and red blood cell counts, hemoglobin content, platelet count, accompanied by elevated red cell distribution width percentage were observed, supporting the inflammatory status. Histopathological changes represented by hematoxylin&eosin, immunohistochemistry staining for Bax&Bcl2, and transmission electron microscopy supported the negative impacts of gasoline fumes compared to the control group. Fenugreek seeds supplementation with gasoline exposure showed pronounced alleviation of lung biochemical and histopathological changes compared to the gasoline-exposed group.
In silico validation of RNA-Seq results can identify gene fusions with oncogenic potential in glioblastoma
Authors: Hernandez Ainhoa , Muñoz-Mármol Ana Maria , Esteve-Codina Anna , Alameda Francesc , Carrato Cristina , Pineda Estela , Arpí-Lluciá Oriol , Martinez-García Maria , Mallo Mar , Gut Marta , del Barco Sonia , Gallego Oscar , Dabad Marc , Mesia Carlos , Bellosillo Beatriz , Domenech Marta , Vidal Noemí , Aldecoa Iban , de la Iglesia Nuria , Balana Carmen
Published: August 2022

RNA-Sequencing (RNA-Seq) can identify gene fusions in tumors, but not all these fusions have functional consequences. Using multiple data bases, we have performed an in silico analysis of fusions detected by RNA-Seq in tumor samples from 139 newly diagnosed glioblastoma patients to identify in-frame fusions with predictable oncogenic potential. Among 61 samples with fusions, there were 103 different fusions, involving 167 different genes, including 20 known oncogenes or tumor suppressor genes (TSGs), 16 associated with cancer but not oncogenes or TSGs, and 32 not associated with cancer but previously shown to be involved in fusions in gliomas. After selecting in-frame fusions able to produce a protein product and running Oncofuse, we identified 30 fusions with predictable oncogenic potential and classified them into four non-overlapping categories: six previously described in cancer; six involving an oncogene or TSG; four predicted by Oncofuse to have oncogenic potential; and 14 other in-frame fusions. Only 24 patients harbored one or more of these 30 fusions, and only two fusions were present in more than one patient: FGFR3::TACC3 and EGFR::SEPTIN14. This in silico study provides a good starting point for the identification of gene fusions with functional consequences in the pathogenesis or treatment of glioblastoma.
Controlled drug delivery and cell adhesion for bone tissue regeneration by Keplerate polyoxometalate (Mo132)/metronidazole/PMMA scaffolds
Authors: Taghiyar Hamid , Yadollahi Bahram , Kajani Abolghasem Abbasi
Published: August 2022

The aim of this study is to fabricate a new scaffold appropriate for tissue regeneration with antimicrobial activity and ability of controlled drug delivery. In this regard, scaffold nanofibers were produced using poly (methyl methacrylate) (PMMA), Mo132 as a Keplerate polyoxometalate and metronidazole. The final scaffolds, obtained by electrospinning, represent the intrinsic features including exceptional doubling tensile strength, high hydrophilicity (126 ± 5.2° to 83.9 ± 3.2° for contact angle and 14.18 ± 0.62% to 35.62 ± 0.24% for water uptake), proper bioactivity and cell adhesion. Moreover, the addition of Mo132 and metronidazole enhances the biodegradation rate of resulted scaffolds compared to the pure PMMA membrane. The controlled release of metronidazole over 14 days efficiently inhibits the colonization of anaerobic microorganisms. Overall, the results demonstrate high potential of Mo132 and metronidazole-loaded PMMA scaffold for guided bone regeneration/guided tissue regeneration.
Reliability and accuracy of the torque applied to osteosynthesis screws by maxillofacial surgeons and residents
Authors: Gareb Barzi , van Munster Valerie D. M. , Dijkstra Pieter U. , Bos Ruud R. M. , Vissink Arjan , van Bakelen Nico B. , van Minnen Baucke
Published: August 2022

Applying the right torque to osteosynthesis screws is important for undisturbed bone healing. This study aimed to compare test–retest and intra-individual reliabilities of the torque applied to 1.5 mm and 2.0 mm osteosynthesis screws by residents and oral and maxillofacial surgeons (OMF-surgeons), to define the reference torque intervals, and to compare reference torque interval compliances. Five experienced OMF-surgeons and 20 residents, 5 of each 4 residency years, were included. Each participant inserted six 1.5 × 4 mm and six 2.0 × 6 mm screws into a preclinical model at two test moments 2 weeks apart (T1 and T2). Participants were blinded for the applied torque. Descriptive statistics, reference intervals, and intra-class correlation coefficients (ICC) were calculated. The OMF-surgeons complied more to the reference intervals (1.5 mm screws: 95% and 2.0 mm screws: 100%) than the residents (82% and 90%, respectively; P = 0.009 and P = 0.007) with the ICCs ranging between 0.85–0.95 and 0.45–0.97, respectively. The residents’ accuracy and reliability were inadequate regarding the 1.5 mm screws but both measures improved at T2 for both screw types compared to T1, indicating a learning effect. Training residents and/or verifying the applied torque by experienced OMF-surgeons remains necessary to achieve high accuracy and reliability, particularly for 1.5 mm screws.
Heterosis for capsacinoids accumulation in chili pepper hybrids is dependent on parent-of-origin effect
Authors: Naves Emmanuel Rezende , Scossa Federico , Araújo Wagner L. , Nunes-Nesi Adriano , Fernie Alisdair R. , Zsögön Agustin
Published: August 2022

Heterosis for agronomic traits is a widespread phenomenon that underpins hybrid crop breeding. However, heterosis at the level of cellular metabolites has not yet been fully explored. Some metabolites are highly sought after, like capsaicinoids found in peppers of theCapsicumgenus, which confer the characteristic pungent (‘hot’) flavour of the fruits. We analysed the metabolic profile of the fruit placenta and pericarp of inter- and intra-specific hybrids of two species ofCapsicumpeppers,C. chinense(cv. Habanero and cv. Biquinho) andC. annuumvar.annuum(cv. Jalapeño and cv. Cascadura Ikeda) in complete diallel crosses with reciprocals. The parents and hybrids were grown in a glasshouse and the profile of primary metabolites (sugars, amino acids and organic acids) and capsaicinoids was generated via gas chromatography–time of flight-mass spectrometry (GC–TOF-MS) and ultra-performance liquid chromatography coupled to a mass spectrometer (UPLC-MS), respectively. We found considerable heterotic effects specifically for capsaicinoids accumulation in the fruit placenta of the hybrids, including those derived from non-pungent parents. Furthermore, a large fraction of fruit primary metabolism was influenced by the specific cross combination, with marked parent-of-origin effects, i.e. whether a specific genotype was used as the pistillate or pollen parent. The differences in metabolite levels between the hybrids and their parents provide a snapshot of heterosis for primary and secondary metabolites and may contribute to explain the manifestation of whole-plant heterotic phenotypes.
Algoritmically improved microwave radar monitors breathing more acurrate than sensorized belt
Authors: Czyżewski Andrzej , Kostek Bozena , Kurowski Adam , Narkiewicz Krzysztof , Graff Beata , Odya Piotr , Śmiałkowski Tomasz , Sroczyński Andrzej
Published: August 2022

This paper describes a novel way to measure, process, analyze, and compare respiratory signals acquired by two types of devices: a wearable sensorized belt and a microwave radar-based sensor. Both devices provide breathing rate readouts. First, the background research is presented. Then, the underlying principles and working parameters of the microwave radar-based sensor, a contactless device for monitoring breathing, are described. The breathing rate measurement protocol is then presented, and the proposed algorithm for octave error elimination is introduced. Details are provided about the data processing phase; specifically, the management of signals acquired from two devices with different working principles and how they are resampled with a common processing sample rate. This is followed by an analysis of respiratory signals experimentally acquired by the belt and microwave radar-based sensors. The analysis outcomes were checked using Levene’s test, the Kruskal–Wallis test, and Dunn’s post hoc test. The findings show that the proposed assessment method is statistically stable. The source of variability lies in the person-triggered breathing patterns rather than the working principles of the devices used. Finally, conclusions are derived, and future work is outlined.
Analysis of the association between atrial fibrillation with in-hospital mortality in people admitted for community-acquired pneumonia through an observational, nation-wide, sex-stratified study
Authors: de-Miguel-Yanes José M. , Jiménez-García Rodrigo , Hernández-Barrera Valentín , de-Miguel-Díez Javier , Méndez-Bailón Manuel , López-de-Andrés Ana
Published: August 2022

We aimed to analyze the influence of atrial fibrillation (AF) prior to hospital admission (“prevalent”) and AF diagnosed during hospital admission (“incident”) on in-hospital mortality (IHM) in women and men admitted for community-acquired pneumonia (CAP) in Spain (2016–2019). We used the Spanish Register of Specialized Care‐Basic Minimum Database. We analyzed 519,750 cases of CAP in people ≥ 18 years (213,631 women (41.1%)), out of which people with prevalent AF represented 23.75% (N = 123,440), whereas people with incident AF constituted 0.60% (N = 3154). Versus no AF, crude IHM was significantly higher for prevalent AF (15.24% vs. 11.40%,p < 0.001) and for incident AF (23.84% vs. 12.24%,p < 0.001). After propensity score marching, IHM in women and men with prevalent AF neared IHM in women and men with no AF (15.72% vs. 15.52%,p = 0.425; and 14.90% vs. 14.99%,p = 0.631, respectively), but IHM in women and men with incident AF was higher than IHM in women and men with no AF (24.37% vs. 13.36%,p < 0.001; and 23.94% vs. 14.04%,p < 0.001, respectively). Male sex was associated with a higher IHM in people with prevalent AF (OR 1.06; 95% CI 1.02–1–10), but not in people with incident AF (OR 0.93; 95% CI 0.77–1–13). AF diagnosed during hospital admission was associated with a higher IHM, irrespectively of sex.
Evolutionary analysis of swimming speed in early vertebrates challenges the ‘New Head Hypothesis’
Authors: Ferrón Humberto G. , Donoghue Philip C. J.
Published: August 2022

The ecological context of early vertebrate evolution is envisaged as a long-term trend towards increasingly active food acquisition and enhanced locomotory capabilities culminating in the emergence of jawed vertebrates. However, support for this hypothesis has been anecdotal and drawn almost exclusively from the ecology of living taxa, despite knowledge of extinct phylogenetic intermediates that can inform our understanding of this formative episode. Here we analyse the evolution of swimming speed in early vertebrates based on caudal fin morphology using ancestral state reconstruction and evolutionary model fitting. We predict the lowest and highest ancestral swimming speeds in jawed vertebrates and microsquamous jawless vertebrates, respectively, and find complex patterns of swimming speed evolution with no support for a trend towards more active lifestyles in the lineage leading to jawed groups. Our results challenge the hypothesis of an escalation of Palaeozoic marine ecosystems and shed light into the factors that determined the disparate palaeobiogeographic patterns of microsquamous versus macrosquamous armoured Palaeozoic jawless vertebrates. Ultimately, our results offer a new enriched perspective on the ecological context that underpinned the assembly of vertebrate and gnathostome body plans, supporting a more complex scenario characterized by diverse evolutionary locomotory capabilities reflecting their equally diverse ecologies.