bluuebunny/crossref_metadata_2025 huggingface dataset:
Methyl Jasmonate Treatment of Broccoli Enhanced Glucosinolate Concentration, Which Was Retained after Boiling, Steaming, or Microwaving
Authors: Chiu Yu-Chun , Matak Kristen , Ku Kang-Mo
Published: June 2020

Exogenous methyl jasmonate (MeJA) treatment was known to increase the levels of neoglucobrassicin and their bioactive hydrolysis products in broccoli (Brassica oleracea var. italica), but the fate of MeJA-induced glucosinolates (GSLs) after various cooking methods was unknown. This study measured the changes in GSLs and their hydrolysis compounds in broccoli treated with MeJA and the interaction between MeJA and cooking treatments. All cooked MeJA-treated broccoli contained significantly more GSLs than untreated broccoli (p < 0.05). After 5 min of cooking (boil, steam, microwave), MeJA-treated broccoli still contained 1.6- to 2.3-fold higher GSL content than untreated broccoli. Neoglucobrassicin hydrolysis products were also significantly greater in steamed and microwaved MeJA-treated broccoli. The results show that exogenous MeJA treatment increases neoglucobrassicin and its hydrolysis compounds in broccoli even after cooking. Once the positive and negative effects of these compounds are better understood, the results of this experiment can be a valuable tool to help food scientists, nutrition scientists, and dieticians determine how to incorporate raw or cooked broccoli and Brassica vegetables in the diet.
Validation of Rapid Enzymatic Quantification of Acetic Acid in Vinegar on Automated Spectrophotometric System
Authors: Dini Irene , Di Lorenzo Ritamaria , Senatore Antonello , Coppola Daniele , Laneri Sonia
Published: June 2020

Vinegar is produced from the fermentation of agricultural materials and diluted acetic acid (diluted with water to 4–30% by volume) via sequential ethanol and acetic acid fermentation. The concentration of acetic acid must be measured during vinegar production. A Community method for analyzing acetic acid in vinegar is a non-specific method based on the assumption that the total acid concentration of the vinegar is attributable to the acetic acid. It consists of titration with a strong base in the presence of an indicator. This test is laborious and has a time-consuming character. In this work, a highly specific automated enzymatic method was validated, for the first time, to quantify the acetic acid in the wine vinegar, in terms of linearity, precision, repeatability, and uncertainty measurement. The results were compared to the Community method of analysis. Regression coefficient ≅ 1 and the normal distribution of residuals in the ANOVA test confirmed the method’s linearity. LLOD (0.946 ppm) and LLOQ (2.00 ppm) defined the method’s sensitivity. The results of the tested and the Community methods, linearly distributed in the Shapiro–Wilk test, confirmed the method’s repeatability. The few anomalous data in the Huber test were due to random errors. The high selectivity of the enzymatic method, which exclusively measures acetic acid concentration, determined the significant differences between the two tests, examined in the accuracy determination. The enzymatic method can be considered applicable since its precision and uncertainty were lower than the Community method values (relative percentage deviations = 10%). The enzymatic method compared to the Community method reduces the analysis time and the risk of errors due to operators (avoid pipetting errors and wrong calculations), minimizes solvent and the sample consumption and guarantees assay quality through method standardization.
RECQ1 Helicase in Genomic Stability and Cancer
Authors: Debnath Subrata , Sharma Sudha
Published: June 2020

RECQ1 (also known as RECQL or RECQL1) belongs to the RecQ family of DNA helicases, members of which are linked with rare genetic diseases of cancer predisposition in humans. RECQ1 is implicated in several cellular processes, including DNA repair, cell cycle and growth, telomere maintenance, and transcription. Earlier studies have demonstrated a unique requirement of RECQ1 in ensuring chromosomal stability and suggested its potential involvement in tumorigenesis. Recent reports have suggested that RECQ1 is a potential breast cancer susceptibility gene, and missense mutations in this gene contribute to familial breast cancer development. Here, we provide a framework for understanding how the genetic or functional loss of RECQ1 might contribute to genomic instability and cancer.
Computational Analysis of Transcriptomic and Proteomic Data for Deciphering Molecular Heterogeneity and Drug Responsiveness in Model Human Hepatocellular Carcinoma Cell Lines
Authors: Agioutantis Panagiotis C. , Loutrari Heleni , Kolisis Fragiskos N.
Published: June 2020

Hepatocellular carcinoma (HCC) is associated with high mortality due to its inherent heterogeneity, aggressiveness, and limited therapeutic regimes. Herein, we analyzed 21 human HCC cell lines (HCC lines) to explore intertumor molecular diversity and pertinent drug sensitivity. We used an integrative computational approach based on exploratory and single-sample gene-set enrichment analysis of transcriptome and proteome data from the Cancer Cell Line Encyclopedia, followed by correlation analysis of drug-screening data from the Cancer Therapeutics Response Portal with curated gene-set enrichment scores. Acquired results classified HCC lines into two groups, a poorly and a well-differentiated group, displaying lower/higher enrichment scores in a “Specifically Upregulated in Liver” gene-set, respectively. Hierarchical clustering based on a published epithelial–mesenchymal transition gene expression signature further supported this stratification. Between-group comparisons of gene and protein expression unveiled distinctive patterns, whereas downstream functional analysis significantly associated differentially expressed genes with crucial cancer-related biological processes/pathways and revealed concrete driver-gene signatures. Finally, correlation analysis highlighted a diverse effectiveness of specific drugs against poorly compared to well-differentiated HCC lines, possibly applicable in clinical research with patients with analogous characteristics. Overall, this study expanded the knowledge on the molecular profiles, differentiation status, and drug responsiveness of HCC lines, and proposes a cost-effective computational approach to precision anti-HCC therapies.
Comparative Transcriptomics Reveals Gene Families Associated with Predatory Behavior in Photuris femme fatale Fireflies
Authors: McKinley Cheyenne N. , Lower Sarah E.
Published: June 2020

Identifying the basis of phenotypic variation is a key objective of genetics. This work has been mostly limited to model systems with a plethora of genetic manipulation and functional characterization tools. With the development of high-throughput sequencing and new computational tools, it is possible to identify candidate genes related to phenotypic variation in non-model organisms. Fireflies are excellent for studying phenotypic variation because of their diverse and well-characterized behaviors. Most adult fireflies emit a single mating flash pattern and do not eat. In contrast, adult females of many species in the genus Photuris employ multiple flash patterns and prey upon mate-seeking males of other firefly species. To investigate the genetic basis for this variation, we used comparative transcriptomics to identify positively selected genes between a predatory firefly, Photuris sp., and a non-predatory relative, Photuris frontalis, controlling for genes generally under selection in fireflies by comparing to a Photinus firefly. Nine gene families were identified under positive selection in the predatory versus non-predatory Photuris comparison, including genes involved in digestion, detoxification, vision, reproduction, and neural processes. These results generate intriguing hypotheses about the genetic basis for insect behavior and highlight the utility of comparative transcriptomic tools to investigate complex behaviors in non-model systems.
A Comprehensive Cytogenetic Analysis of Several Members of the Family Columbidae (Aves, Columbiformes)
Authors: Kretschmer Rafael , Furo Ivanete de Oliveira , Gomes Anderson José Baia , Kiazim Lucas G. , Gunski Ricardo José , Garnero Analía del Valle , Pereira Jorge C. , Ferguson-Smith Malcolm A. , de Oliveira Edivaldo Herculano Corrêa , Griffin Darren K. , de Freitas Thales Renato Ochotorena , O’Connor Rebecca E.
Published: June 2020

The Columbidae species (Aves, Columbiformes) show considerable variation in their diploid numbers (2n = 68–86), but there is limited understanding of the events that shaped the extant karyotypes. Hence, we performed whole chromosome painting (wcp) for paints GGA1-10 and bacterial artificial chromosome (BAC) probes for chromosomes GGA11-28 for Columbina passerina, Columbina talpacoti, Patagioenas cayennensis, Geotrygon violacea and Geotrygon montana. Streptopelia decaocto was only investigated with paints because BACs for GGA10-28 had been previously analyzed. We also performed phylogenetic analyses in order to trace the evolutionary history of this family in light of chromosomal changes using our wcp data with chicken probes and from Zenaida auriculata, Columbina picui, Columba livia and Leptotila verreauxi, previously published. G-banding was performed on all these species. Comparative chromosome paint and G-banding results suggested that at least one interchromosomal and many intrachromosomal rearrangements had occurred in the diversification of Columbidae species. On the other hand, a high degree of conservation of microchromosome organization was observed in these species. Our cladistic analysis, considering all the chromosome rearrangements detected, provided strong support for L. verreauxi and P. cayennensis, G. montana and G. violacea, C. passerina and C. talpacoti having sister taxa relationships, as well as for all Columbidae species analyzed herein. Additionally, the chromosome characters were mapped in a consensus phylogenetic topology previously proposed, revealing a pericentric inversion in the chromosome homologous to GGA4 in a chromosomal signature unique to small New World ground doves.
Nitrate Signaling, Functions, and Regulation of Root System Architecture: Insights from Arabidopsis thaliana
Authors: Asim Muhammad , Ullah Zia , Xu Fangzheng , An Lulu , Aluko Oluwaseun Olayemi , Wang Qian , Liu Haobao
Published: June 2020

Root system architecture (RSA) is required for the acquisition of water and mineral nutrients from the soil. One of the essential nutrients, nitrate (NO3−), is sensed and transported by nitrate transporters NRT1.1 and NRT2.1 in the plants. Nitrate transporter 1.1 (NRT1.1) is a dual-affinity nitrate transporter phosphorylated at the T101 residue by calcineurin B-like interacting protein kinase (CIPKs); it also regulates the expression of other key nitrate assimilatory genes. The differential phosphorylation (phosphorylation and dephosphorylation) strategies and underlying Ca2+ signaling mechanism of NRT1.1 stimulate lateral root growth by activating the auxin transport activity and Ca2+-ANR1 signaling at the plasma membrane and the endosomes, respectively. NO3− additionally functions as a signal molecule that forms a signaling system, which consists of a vast array of transcription factors that control root system architecture that either stimulate or inhibit lateral and primary root development in response to localized and high nitrate (NO3−), respectively. This review elucidates the so-far identified nitrate transporters, nitrate sensing, signal transduction, and the key roles of nitrate transporters and its downstream transcriptional regulatory network in the primary and lateral root development in Arabidopsis thaliana under stress conditions.
Rockfall Hazard Assessment in Volcanic Regions Based on ISVS and IRVS Geomechanical Indices
Authors: González de Vallejo Luis I. , Hernández-Gutiérrez Luis E. , Miranda Ana , Ferrer Mercedes
Published: June 2020

In volcanic regions, rockfalls represent a major hazard strongly conditioned by the geomechanical behaviour of volcanic materials, the geomorphological characteristics of the relief and the climatic conditions. Volcanic rocks possess very different properties to those of other lithological groups, presenting highly heterogeneous geomechanical behaviours. Nevertheless, they have received little research attention in the field of geological and geotechnical engineering. To date, the application of geomechanical classifications to characterise and estimate volcanic slope stability has not yielded reliable results, indicating the need to establish specific criteria for these rocks. Consequently, we developed indices to estimate rockfall susceptibility, hazard and risk in volcanic slopes. The index of susceptibility for volcanic slopes (ISVS) is designed to estimate slope susceptibility to instability, which is related to the level of hazard, while the index of risk for volcanic slopes (IRVS) is designed to estimate the level of risk as a function of the potential damage or economic loss caused as a result of rockfalls on slopes. Both indices were developed in order to provide an easily applied procedure that facilitates the adoption of short-term preventive measures against rockfalls. The indices were applied in Tenerife (Canary Islands), which presents exceptional conditions for analysing slope stability in volcanic rocks because of its mountainous orography with very steep slopes and a wide variety of materials. These conditions have frequently precipitated slope instability, causing significant damage to housing, beaches, roads and other infrastructures. After applying these indices to a number of slopes representative of the island’s wide variety of geological, geomorphological and climatic conditions, the results obtained were compared with the actual behaviour of the slopes, determined from extensive rockfall inventory data and in situ geomechanical surveys.
In Situ Block Size Distribution Aimed at the Choice of the Design Block for Rockfall Barriers Design: A Case Study along Gardesana Road
Authors: Umili Gessica , Bonetto Sabrina Maria Rita , Mosca Pietro , Vagnon Federico , Ferrero Anna Maria
Published: June 2020

When studying rockfall phenomena, a single value of the block volume is not sufficient to take into account the natural variability of the geometrical features (orientation, spacing, persistence) of the discontinuity sets. Different approaches for obtaining cumulative distributions of potentially detachable block volumes are compared. A highly fractured rock mass outcropping along the western Lake Garda (Italy), consisting of prevailing limestone and interbedded marls, is studied in detail from geological and geostructural points of view. Then, a representative rock face has been selected and analyzed with traditional and non-contact survey methods to identify the main discontinuity sets and to collect spacing samples. Based on these data, in situ block size distributions for different combinations of sets are built following statistically-based approaches, without the use of a Discrete Fracture Network (DFN) generator. The validation of the obtained distributions is attempted based on the detached block surveyed at the foot of the slope. However, in this particular case study, the detached blocks cover only a minimal volume range compared to both theoretical values and visible rockfall scars. The fallen rock blocks have a marginal role in design block determination, since their volume depends on geological discontinuities (bedding and fractures) and could be affected by other processes after the detachment (e.g., fragmentation). The procedure here described should be standard practice in the study of rockfall events, and it should be uniform in European standards such as Eurocodes. Future developments should involve the scientific community for setting the percentiles of the probability distribution to be considered for block design definition.
Determiners in the Consumer’s Purchase Decision Process in Ecotourism Contexts: A Portuguese Case Study
Authors: Roseta Paulo , Sousa Bruno Barbosa , Roseta Lara
Published: June 2020

Purpose: This paper entails a reflection on sustainability and specific tourism contexts (i.e., ecotourism). It focusses mainly on local communities’ determiners in the consumer’s purchase decision process in ecotourism. This research aimed at understanding the determiners in the process of consumer decisions in ecotourism contexts, as well as verifying the growing importance of ecotourism. Methodology: A questionnaire was conducted (with ecotourism specific contexts), in Portugal. Portugal is a tourist destination that has gained notoriety and recognition in recent years. In total, 197 questionnaires were collected, aiming for a deeper understanding of the real social, economic, cultural and environmental impacts resulting from the uncontrolled development of tourism. This study aimed at testing the proposed conceptual model empirically (i.e., the relationship between destination image and post-purchase behaviour). Findings: Sustainability must be looked at as a vital need for society in general, as well as for the tourism sector. Ecotourism is the natural evolution of tourism employing care for the environment, culture and local traditions, while promoting environmental education. Research Limitations: The results obtained are limited, considering the availability of most accommodation units in the ecotourism segment in Portugal (e.g., hotels). As next steps, it is advisable that hotel units come to realise the pertinence of such research as they are the main beneficiaries of these initiatives, being then recommended that they collaborate with the process of completing surveys. Originality/Value: Managers and ecotourists can use the outcomes of this study to gain an in-depth understanding of customer experiences (i.e., sustainability experiences), to develop effective marketing strategies, and to create an operational environment that can maximise customers’ perceived experiential values. Destination image plays a fundamental part in terms of experience and the tourist’s own satisfaction, with a need to improve the degree of affectivity towards the hotel unit.
Imaging Buried Archaeological Features through Ground Penetrating Radar: The Case of the Ancient Saepinum (Campobasso, Italy)
Authors: Cozzolino Marilena , Gentile Vincenzo , Giordano Claudia , Mauriello Paolo
Published: June 2020

The archaeological area of Saepinum is considered the symbol of the history of Roman civilization in Molise region (Italy). It was a Samnite commercial forum and service center, then it became a Roman municipium, and, later, it was transformed into a medieval and modern rural village. Although the archaeological excavations brought to light different important public buildings, such as the theater, the forum, the basilica, different temples, and the main streets, today, there is still much to discover and study inside the well-preserved city walls. For this purpose, a ground penetrating radar (GPR) survey was realized in the space between the theater and the decumanus, allowing imaging of a complex regular pattern of archaeological features belonging to thermal buildings still buried in the soil.
Modeling Public Opinion Reversal Process with the Considerations of External Intervention Information and Individual Internal Characteristics
Authors: Chen Tinggui , Wang Yulong , Yang Jianjun , Cong Guodong
Published: June 2020

With the rapid development of “we media” technology, external information about the same sudden hot social event is often involved repetitiously, leading to frequent public opinion reversal. However, the phenomenon of public opinion reversal process usually has a long-lasting duration and spreads wide, making the event itself attract the widespread attention of ordinary people. Focusing on the public opinion reversal process of sudden social hot topic (a popular and widely discussed issue), this paper firstly identifies the internal and external factors that affect the reversal, namely individual internal characteristics and external intervention information. Secondly, information intensity and the amount of information perceived by individuals are introduced to describe the impact of external intervention information on the public opinion reversal. Thirdly, the parameters of individual attention and conservation are used to describe the process of individual’s selection of external information, so as to reveal the influence of the internal characteristics on public opinion reversal, and then build a public opinion reversal model. Fourthly, the effects of information intensity and individual attention, as well as individual conservation on the process of public opinion reversal are analyzed by simulation experiment. Simulation results show that: (1) the intensity of external intervention information affects the direction and degree of public opinion reversal; (2) when individual conservation is strong or individual attention is weak, even if external intervention information is strong, there will still be no obvious reversal of public opinion. Subsequently, the rationality and effectiveness of the proposed model are verified by a real case. Finally, some recommendations and policy implications are also given.
Immediate Effect of Balance Taping Using Kinesiology Tape on Dynamic and Static Balance after Ankle Muscle Fatigue
Authors: Choi Hyun-Su , Lee Jung-Hoon
Published: June 2020

The objective of this study was to investigate whether ankle balance taping (ABT) applied after muscle fatigue-inducing exercise can cause immediate improvements in dynamic and static balance. A total of 31 adults (16 males and 15 females) met the inclusion criteria. The experiment was designed using a single-blinded, randomized controlled trial. Changes in static and dynamic balance were measured before and after inducing muscle fatigue in the ankles and after ABT or ankle placebo taping (APT). After ankle muscle fatigue-inducing exercise, both the ABT and APT groups showed significant increases in surface area ellipses in the static state with eyes open (p < 0.05), and significant increases in surface area ellipses in the static and dynamic states with eyes closed (both p < 0.05). After taping of the fatigued ankle, surface area ellipses decreased significantly when eyes were open and closed in the static and dynamic states, but only in the ABT group (p < 0.05). Static balance was significantly different between groups (eyes open, 36.2 ± 86; eyes closed, 22.9 ± 46.7). Dynamic balance was significantly different between groups (eyes open, 68.6 ± 152.1; eyes closed, 235.8 ± 317.6). ABT may help prevent ankle injuries in individuals who experience muscle fatigue around the ankles after sports and daily activities.
Intra-Laboratory Evaluation of Luminescence Based High-Throughput Serum Bactericidal Assay (L-SBA) to Determine Bactericidal Activity of Human Sera against Shigella
Authors: Rossi Omar , Molesti Eleonora , Saul Allan , Giannelli Carlo , Micoli Francesca , Necchi Francesca
Published: June 2020

Despite the huge decrease in deaths caused by Shigella worldwide in recent decades, shigellosis still causes over 200,000 deaths every year. No vaccine is currently available, and the morbidity of the disease coupled with the rise of antimicrobial resistance renders the introduction of an effective vaccine extremely urgent. Although a clear immune correlate of protection against shigellosis has not yet been established, the demonstration of the bactericidal activity of antibodies induced upon vaccination may provide one means of the functionality of antibodies induced in protecting against Shigella. The method of choice to evaluate the complement-mediated functional activity of vaccine-induced antibodies is the Serum Bactericidal Assay (SBA). Here we present the development and intra-laboratory characterization of a high-throughput luminescence-based SBA (L-SBA) method, based on the detection of ATP as a proxy of surviving bacteria, to evaluate the complement-mediated killing of human sera. We demonstrated the high specificity of the assay against a homologous strain without any heterologous aspecificity detected against species-related and non-species-related strains. We assessed the linearity, repeatability and reproducibility of L-SBA on human sera. This work will guide the bactericidal activity assessment of clinical sera raised against S. sonnei. The method has the potential of being applicable with similar performances to determine the bactericidal activity of any non-clinical and clinical sera that rely on complement-mediated killing.
Epidemiological and Microbiological Aspects of the Peritonsillar Abscess
Authors: Slouka David , Hanakova Jana , Kostlivy Tomas , Skopek Petr , Kubec Vojtech , Babuska Vaclav , Pecen Ladislav , Topolcan Ondřej , Kucera Radek
Published: June 2020

Peritonsillar abscess (PTA) is the most common complication of tonsillitis. Cultivation usually reveals a wide spectrum of aerobic and anaerobic microbiota. This retrospective study compared PTA incidence and the spectrum of individual microbial findings in groups of patients divided by gender, age, and season. Of the 966 samples cultivated, a positive cultivation finding was detected in 606 patients (62.73%). Cultivation findings were negative in 360 (37.27%), meaning no pathogen was present or only common microbiota was cultivated. The highest incidence of PTA was found in group I patients (19–50 years) (p ≤ 0.0001) and the most frequently cultured pathogens was Streptococcus pyogenes (36.23%). Gender seemed to have an influence on the results, with higher incidence found in males (p ≤ 0.0001). The analysis of correlation between PTA incidence and season did not yield statistically significant results (p = 0.4396) and no statistically significant differences were observed in individual pathogen frequency. PTA had a higher incidence in adult males and a slightly higher incidence in girls in childhood. The following findings are clinically significant and have implications for antibiotic treatment strategy: (1) the most frequently cultivated pathogen was Streptococcus pyogenes; (2) an increased incidence of anaerobes was proven in the oldest group (>50 years).
A Focus Group Interview Study of the Experience of Stress amongst School-Aged Children in Sweden
Authors: Warghoff Alexandra , Persson Sara , Garmy Pernilla , Einberg Eva-Lena
Published: June 2020

The study explored experiences of stress in children aged 10–12. An inductive qualitative design was used. Ten focus group interviews were conducted with Swedish schoolchildren (n = 42) aged 10–12. The interviews were audio-recorded, transcribed verbatim, and analyzed using qualitative content analysis. The study identified one theme—“Body and mind react”—and three subthemes: (1) Friendships matters, (2) Parental stress affect, and (3) Trying to do my best. Stress often occurred in the children’s everyday environment. The children had experienced how stress could be expressed both physically and mentally, inside and outside school. The children also had the experience of seeing people in their environment being stressed, which could create feelings that affected the children. The experience of the children was also that stress can sometimes be good. Stress related to peers, family, school, and society was commonly experienced by children aged 10–12. Both positive and negative stress was reported.
Social Participation in Relation to Technology Use and Social Deprivation: A Mixed Methods Study Among Older People with and without Dementia
Authors: Gaber Sophie N. , Nygård Louise , Brorsson Anna , Kottorp Anders , Charlesworth Georgina , Wallcook Sarah , Malinowsky Camilla
Published: June 2020

Social participation is a modifiable determinant for health and wellbeing among older people; however, social participation is increasingly dependent on technology use. This study investigated social participation in relation to Everyday Technology use and social deprivation of the living environment, among older people with and without dementia in the United Kingdom. Sixty-four people with dementia and sixty-four people without dementia were interviewed using standardized questionnaires: The Participation in ACTivities and Places OUTside Home Questionnaire and Everyday Technology Use Questionnaire. A mixed methods approach integrated statistical analyses and content analysis of free-text responses, through data visualizations. Small, statistically significant associations were found between social participation and Everyday Technology use outside home, for participants with dementia (Rs = 0.247; p = 0.049) and without dementia (Rs = 0.343; p = 0.006). A small, statistically significant association was identified between social participation and social deprivation in the living environment, among only participants with dementia (Rs = 0.267, p = 0.033). The content analysis and graphical joint display revealed motivators, considerations that require extra attention, and strategies for managing social participation. The results underline how Everyday Technology use can be assistive to social participation but also the need to consider social deprivation of the living environment, especially among people with dementia.
Electroconvulsive Therapy in Super Refractory Status Epilepticus: Case Series with a Defined Protocol
Authors: García-López Beatriz , Gómez-Menéndez Ana Isabel , Vázquez-Sánchez Fernando , Pérez-Cabo Eva , Isidro-Mesas Francisco , Zabalegui-Pérez Arturo , Muñoz-Siscart Ignacio , Lloria-Gil María Carmen , Soto-Cámara Raúl , González-Bernal Jerónimo J. , González-Santos Josefa , Aguilar-Parra José M. , Trigueros Rubén , López-Liria Remedios , Kjær Troels Wesenberg
Published: June 2020

Super-refractory status epilepticus (SRSE) represents a neurological emergency that is characterized by a lack of response to the third line of antiepileptic treatment, including intravenous general anesthetics. It is a medical challenge with high morbidity and mortality. Electroconvulsive therapy (ECT) has been recommended as a nonpharmacologic option of treatment after other alternatives are unsuccessful. Its effect on the cessation of SRSE has been minimally investigated. The objective of this article is to analyze the effect of ECT on SRSE. For this purpose, a multidisciplinary team created a protocol based on clinical guidelines similar to those described previously by Ray et al. (2017). ECT was applied to six patients with SRSE after the failure of antiepileptic treatment and pharmacologic coma.The objective of each ECT session was to elicit a motor seizure for at least 20 s. SRSE was resolved in all patients after several days of treatment, including ECT as a therapy, without relevant adverse effects. Thus, ECT is an effective and feasible option in the treatment of SRSE, and its place in the algorithm in treatment should be studied due to the uncommon adverse effects and the noninvasive character of the therapy.
Monitoring Environmental Parameters with Oil and Gas Developments in the Permian Basin, USA
Authors: Nelson Robert , Heo Joonghyeok
Published: June 2020

This study evaluates the groundwater qualities and environmental changes to obtain information on the groundwater contamination in the Permian Basin, Texas. Coupled with the U.S. government’s open data, these analyses can identify regions where environmental change could have affected groundwater quality. A total of thirty-six wells were selected within the six counties: Andrews, Martin, Ector, Midland, Crane, and Upton. Spatial distribution maps were created for six different parameters: pH, total dissolved solids (TDS), chloride, fluoride, nitrate, and arsenic. Total groundwater quality maps incorporate all the contaminants and denote regions of poor, medium, and optimum conditions. To identify spatial changes in groundwater quality, maps were separated into two different time intervals, 1992–2005 and 2006–2019. We found that groundwater contamination resulted primarily from the mobilization of the contaminant from anthropogenic activities such as chemical fertilizers, oil and gas developments. Overall, groundwater quality decreased during the study period from 1992 to 2019 as population and urban growth began to develop in the Permian Basin. This study contributes on understanding of the response of groundwater quality associated with environmental change in the Permian Basin. Therefore, this research provides important information for groundwater managements in developing plans for the use of water resource in the future.
Emotional Bias Modification in Youths with Attention Deficit Hyperactivity Disorder (ADHD): New Research Vista
Authors: Melvyn Zhang , Chow Aloysius , Vallabhajosyula Ranganath , Fung Daniel SS
Published: June 2020

Whilst cognitive bias modification was initially used in the treatment of anxiety disorders, it is also currently being used for the treatment of other psychopathologies. In fact, cognitive bias modification has been especially well-investigated amongst children and adolescents. A recent review suggests some evidence for the modification of interpretative biases amongst children with neurodevelopment disorders. There have since been other studies reporting of the existence of other cognitive biases, such as emotional biases, amongst individuals with attention deficit hyperactivity disorder (ADHD). This perspective article will discuss the epidemiology of ADHD and the nature of emotional biases that are present amongst individuals with ADHD. This perspective article also reviewed some of the studies that have assessed and modified emotional biases in individuals with ADHD. A total of three studies have been identified from the published literature that provide evidence for targeting emotional biases amongst individuals with ADHD. These studies provide us with preliminary evidence for the effectiveness of modifying emotional biases and how it could help in ameliorating symptoms related to emotional dysregulation. There needs to be future research in this area with further evidence supporting the effectiveness of modifying emotional biases. It is also crucial for future research to determine which of these tools is best at detecting such biases, and which of these tools are versatile enough and non-invasive that they could safely be implemented for both research and clinical needs.
Functional Gastrointestinal Disorders in Outpatients Aged up to 12 Months: A French Non-Interventional Study
Authors: Campeotto Florence , Barbaza Marie-Odile , Hospital Veronique
Published: June 2020

Background: The aim of this study was to estimate the frequency of functional gastrointestinal disorders (FGIDs) in infants aged up to 12 months according to the new ROME IV criteria defining these disorders, and to describe the management of FGIDs in France. Methods: This French non-interventional, cross-sectional, and multicenter study was conducted among private-outpatient physicians who each included four consecutive patients aged up to 12 months. The frequency of FGIDs was described using the ROME IV criteria versus clinicians’ diagnosis. The characteristics of infants with and without FGID were compared, and the management of the FGIDs was described. Results: In the 1722 infants analyzed, the following frequencies were observed according to the ROME IV criteria versus the physicians’ diagnosis: regurgitation 41% versus 45%; colic 18% versus 30%; constipation 9% versus 19%; diarrhea 3% versus 8%. Of note, FGID infants were less frequently exclusively breastfeeding at the maternity hospital (p < 0.001), were introduced to cow’s milk earlier after leaving the maternity hospital (p < 0.001), and more frequently had symptoms suggestive of cow’s milk protein allergy (p < 0.001). Physicians frequently recommended an adapted infant formula (in 77% to 82% of cases depending on the FGID diagnosed) and prescribed a specific treatment in 51% to 66% of infants (probiotics: 35% to 64%). Conclusions: This real-world study confirms the high frequency of FGIDs in infants in France, and provides new information regarding the characteristics of FGID infants.
Prenatal Mercury Exposure in Pregnant Women from Suriname’s Interior and Its Effects on Birth Outcomes
Authors: Baldewsingh Gaitree K. , Wickliffe Jeffrey K. , van Eer Edward D. , Shankar Arti , Hindori-Mohangoo Ashna D. , Harville Emily W. , Covert Hannah H. , Shi Lizheng , Lichtveld Maureen Y. , Zijlmans Wilco C.W.R.
Published: June 2020

Prenatal mercury (Hg) exposure was determined in a sub-cohort of the Caribbean Consortium for Environmental and Occupational Health’s environmental epidemiologic prospective cohort study of pregnant women living in Suriname’s interior. The associations between Hg exposure, low birth weight (LBW, <2500 g) and preterm birth (PTB, <37 weeks) were explored. Correlation analysis, Fisher’s exact test and logistic regression analyses were conducted to evaluate the associations between maternal hair Hg levels and birth weight, LBW and PTB, and between potential confounders, LBW and PTB, respectively. Among 204 singleton births were 198 live births, five stillbirths and one miscarriage. The mean participant age was 26 years; 15.7% of participants had PTBs and 8.1% delivered a child with a LBW. The median hair Hg level was 3.48 μg/g hair. Low hair Hg exposure, based on lowest tertile < 2.34 μg/g, was associated with LBW (OR = 7.2; 95% CI 1.5–35.6; p = 0.015); this association was independent of maternal age, ethnic background, household income and village location, and no correlation was found between hair Hg and PTB. Young maternal age was associated with PTB (RR = 5.09, 95% CI: 1.92–13.85; p = 0.0004) while maternal age was not associated with hair Hg or LBW. The impact of prenatal Hg exposure on pediatric neurodevelopment is currently being evaluated in the infant sub-cohort.
Oral Microbiome of Children Living in an Isolated Area in Myanmar
Authors: Nomura Yoshiaki , Otsuka Ryoko , Hasegawa Ryo , Hanada Nobuhiro
Published: June 2020

Several studies have shown that the oral microbiome is related to systemic health, and a co-relation with several specific diseases has been suggested. The oral microbiome depends on environmental- and community-level factors. In this observational study, the oral microbiomes of children of isolated mountain people were analyzed with respect to the core oral microbiome and etiology of dental caries. We collected samples of supragingival plaque from children (age 9–13) living in the Chin state of Myanmar. After DNA extraction and purification, next-generation sequencing of the V3–V4 hypervariable regions of the 16S rRNA was conducted. From thirteen subjects, 263,458 valid reads and 640 operational taxonomic units were generated at a 97% identity cut-off value. At the phylum level, Proteobacteria was the most abundant, followed by Firmicutes and Bacteroides. Forty-four bacteria were detected in total from all the subjects. For children without dental caries, Proteobacteria was abundant. In contrast, in children with dental caries, Firmicutes and Bacteroides were abundant. The oral microbiome of children living in an isolated area may be affected by environmental- and community-level factors. Additionally, the composition of the oral microbiome may affect the risk of dental caries.
Caregivers of Patients with Hematological Malignancies within Home Care: A Phenomenological Study
Authors: Capodanno Isabella , Rocchi Mirta , Prandi Rossella , Pedroni Cristina , Tamagnini Enrica , Alfieri Pierluigi , Merli Francesco , Ghirotto Luca
Published: June 2020

The role of caregivers in homecare settings is relevant to the patient’s wellbeing and quality of life. This phenomenon is well described in the literature for the oncological setting but not specifically for that of hematological malignancies. The aim of this study was to explore the experience of primary caregivers of patients with hematological malignancies within home care. We conducted a phenomenological study based on interviews with 17 primary caregivers of hematological patients. Analysis of the contents led to the identification of five main themes. Perhaps, the innovative aspects of this study can be summarized in three points: This service was demonstrated to fulfil the ethical aspects of providing the patient with a dignified accompaniment to the end of life. Secondly, the efficiency of the service and the benefit are directly dependent on the caregivers’ wellbeing, so knowledge of the dynamics and emotions involved can lead to the development and implementation of programs for hematological malignancies. Lastly, a collaborative caregivers–professionals relationship can improve a sense of accomplishment for all parties involved, lessening the family’s frustration related to not having done their best. Home care brings significant benefits for both the patient and the caregivers and fulfils the ethical obligation of providing the patient dignified end-of-life care.
The Influence of Custom-Milled Framework Design for an Implant-Supported Full-Arch Fixed Dental Prosthesis: 3D-FEA Study
Authors: Tribst João Paulo Mendes , Dal Piva Amanda Maria de Oliveira , Lo Giudice Roberto , Borges Alexandre Luiz Souto , Bottino Marco Antonio , Epifania Ettore , Ausiello Pietro
Published: June 2020

The current study aimed to evaluate the mechanical behavior of two different maxillary prosthetic rehabilitations according to the framework design using the Finite Element Analysis. An implant-supported full-arch fixed dental prosthesis was developed using a modeling software. Two conditions were modeled: a conventional casted framework and an experimental prosthesis with customized milled framework. The geometries of bone, prostheses, implants and abutments were modeled. The mechanical properties and friction coefficient for each isotropic and homogeneous material were simulated. A load of 100 N load was applied on the external surface of the prosthesis at 30° and the results were analyzed in terms of von Mises stress, microstrains and displacements. In the experimental design, a decrease of prosthesis displacement, bone strain and stresses in the metallic structures was observed, except for the abutment screw that showed a stress increase of 19.01%. The conventional design exhibited the highest stress values located on the prosthesis framework (29.65 MPa) between the anterior implants, in comparison with the experimental design (13.27 MPa in the same region). An alternative design of a stronger framework with lower stress concentration was reported. The current study represents an important step in the design and analysis of implant-supported full-arch fixed dental prosthesis with limited occlusal vertical dimension.
Understanding Needs Satisfaction and Frustration in Young Athletes: Factor Structure and Invariance Analysis
Authors: Monteiro Diogo , Cid Luís , Teixeira Diogo S. , Fonseca Teresa , Duarte-Mendes Pedro , Silva Luís M. , Rodrigues Filipe
Published: June 2020

Sports research has been focused on the assessment of basic needs satisfaction, considering its absence as a representation of needs frustration. However, recent findings have suggested needs satisfaction and frustration as asymmetrical factors leading to differentiated outcomes. An accurate measurement of needs poses itself as a crucial aspect, facilitating coaches’ understanding of athlete’s motivational processes. This study aimed to examine the psychometric proprieties of the Basic Psychological Needs Satisfaction and Frustration Scale (BPNSFS) in a sample of Portuguese athletes. A multigroup analysis was conducted of gender, sport type, age, and years of sports practice. Additionally, needs satisfaction and needs frustration were tested as predictors of behavioral regulations examining the nomological validity of the BPNSFS. Data from 594 Portuguese athletes (38.6% female; Mage = 15.21; SD = 0.97) that represent two different sports (football and swimming) were analyzed. Confirmatory factor analysis and structural equation modeling procedures were followed to test the factor structure and nomological validity of the scale, respectively. Analyses indicated that the six-factor model provided an adequate fit (Comparative Fit Index = 0.947, Tucker–Lewis Index = 0.936, Standardized Root Mean Square = 0.039, Root Mean Square Error of Approximation = 0.048 (CI 90% = 0.043, 0.054)). Moreover, the multigroup analysis suggested invariance in the observed structure across groups. In addition, findings indicated a strong prediction between needs satisfaction and autonomous forms of motivation, whereas needs frustration predicted significantly controlled forms of motivation. The sport-adapted BPNSFS in a sample of Portuguese athletes seemed to be an adequate measure for the assessment of basic psychological needs satisfaction and frustration. Our findings suggested that this scale may be worth testing in future research in the sport context.
Short-Term Effects of Ambient Ozone, PM2.5, and Meteorological Factors on COVID-19 Confirmed Cases and Deaths in Queens, New York
Authors: Adhikari Atin , Yin Jingjing
Published: June 2020

The outbreak of coronavirus disease 2019 (COVID-19), caused by the virus SARS-CoV-2, has been rapidly increasing in the United States. Boroughs of New York City, including Queens county, turn out to be the epicenters of this infection. According to the data provided by the New York State Department of Health, most of the cases of new COVID-19 infections in New York City have been found in the Queens county where 42,023 people have tested positive, and 3221 people have died as of 20 April 2020. Person-to-person transmission and travels were implicated in the initial spread of the outbreaks, but factors related to the late phase of rapidly spreading outbreaks in March and April are still uncertain. A few previous studies have explored the links between air pollution and COVID-19 infections, but more data is needed to understand the effects of short-term exposures of air pollutants and meteorological factors on the spread of COVID-19 infections, particularly in the U.S. disease epicenters. In this study, we have focused on ozone and PM2.5, two major air pollutants in New York City, which were previously found to be associated with respiratory viral infections. The aim of our regression modeling was to explore the associations among ozone, PM2.5, daily meteorological variables (wind speed, temperature, relative humidity, absolute humidity, cloud percentages, and precipitation levels), and COVID-19 confirmed new cases and new deaths in Queens county, New York during March and April 2020. The results from these analyses showed that daily average temperature, daily maximum eight-hour ozone concentration, average relative humidity, and cloud percentages were significantly and positively associated with new confirmed cases related to COVID-19; none of these variables showed significant associations with new deaths related to COVID-19. The findings indicate that short-term exposures to ozone and other meteorological factors can influence COVID-19 transmission and initiation of the disease, but disease aggravation and mortality depend on other factors.