bluuebunny/crossref_metadata_2025 huggingface dataset:
Dyslipidemia and Inflammation as Hallmarks of Oxidative Stress in COVID-19: A Follow-Up Study
Authors: Aparisi Álvaro , Martín-Fernández Marta , Ybarra-Falcón Cristina , Gil José Francisco , Carrasco-Moraleja Manuel , Martínez-Paz Pedro , Cusácovich Iván , Gonzalo-Benito Hugo , Fuertes Raúl , Marcos-Mangas Marta , Iglesias-Echeverría Carolina , San Román J. Alberto , Tamayo Eduardo , Andaluz-Ojeda David , Tamayo-Velasco Álvaro
Published: December 2022

Recent works have demonstrated a significant reduction in cholesterol levels and increased oxidative stress in patients with coronavirus disease 2019 (COVID-19). The cause of this alteration is not well known. This study aimed to comprehensively evaluate their possible association during the evolution of COVID-19. This is an observational prospective study. The primary endpoint was to analyze the association between lipid peroxidation, lipid, and inflammatory profiles in COVID-19 patients. A multivariate regression analysis was employed. The secondary endpoint included the long-term follow-up of lipid profiles. COVID-19 patients presented significantly lower values in their lipid profile (total, low, and high-density lipoprotein cholesterol) with greater oxidative stress and inflammatory response compared to the healthy controls. Lipid peroxidation was the unique oxidative parameter with a significant association with the total cholesterol (OR: 0.982; 95% CI: 0.969–0.996; p = 0.012), IL1-RA (OR: 0.999; 95% CI: 0.998–0.999; p = 0.021) IL-6 (OR: 1.062; 95% CI: 1.017–1.110; p = 0.007), IL-7 (OR: 0.653; 95% CI: 0.433–0.986; p = 0.042) and IL-17 (OR: 1.098; 95% CI: 1.010–1.193; p = 0.028). Lipid abnormalities recovered after the initial insult during long-term follow-up (IQR 514 days); however, those with high LPO levels at hospital admission had, during long-term follow-up, an atherogenic lipid profile. Our study suggests that oxidative stress in COVID-19 is associated with derangements of the lipid profile and inflammation. Survivors experienced a recovery in their lipid profiles during long-term follow-up, but those with stronger oxidative responses had an atherogenic lipid profile.
Fluoxetine Enhances Synaptic Vesicle Trafficking and Energy Metabolism in the Hippocampus of Socially Isolated Rats
Authors: Filipović Dragana , Costina Victor , Findeisen Peter , Inta Dragos
Published: December 2022

Chronic social isolation (CSIS)–induced alternation in synaptic and mitochondrial function of specific brain regions is associated with major depressive disorder (MDD). Despite the wide number of available medications, treating MDD remains an important challenge. Although fluoxetine (Flx) is the most frequently prescribed antidepressant, its mode of action is still unknown. To delineate affected molecular pathways of depressive-like behavior and identify potential targets upon Flx treatment, we performed a comparative proteomic analysis of hippocampal purified synaptic terminals (synaptosomes) of rats exposed to six weeks of CSIS, an animal model of depression, and/or followed by Flx treatment (lasting three weeks of six-week CSIS) to explore synaptic protein profile changes. Results showed that Flx in controls mainly induced decreased expression of proteins involved in energy metabolism and the redox system. CSIS led to increased expression of proteins that mainly participate in Ca2+/calmodulin-dependent protein kinase II (Camk2)-related neurotransmission, vesicle transport, and ubiquitination. Flx treatment of CSIS rats predominantly increased expression of proteins involved in synaptic vesicle trafficking (exocytosis and endocytosis), and energy metabolism (glycolytic and mitochondrial respiration). Overall, these Flx-regulated changes in synaptic and mitochondrial proteins of CSIS rats might be critical targets for new therapeutic development for the treatment of MDD.
miR-145-5p Targets Sp1 in Non-Small Cell Lung Cancer Cells and Links to BMI1 Induced Pemetrexed Resistance and Epithelial–Mesenchymal Transition
Authors: Chang Wen-Wei , Wang Bing-Yen , Chen Shih-Hong , Chien Peng-Ju , Sheu Gwo-Tarng , Lin Ching-Hsiung
Published: December 2022

Pemetrexed is a folic acid inhibitor used as a second-line chemotherapeutic agent for the treatment of locally advanced or metastatic non-small cell lung cancer (NSCLC), which accounts for 85% of lung cancers. However, prolonged treatment with pemetrexed may cause cancer cells to develop resistance. In this study, we found increased expressions of BMI1 (B Lymphoma Mo-MLV insertion region 1 homolog) and Sp1 and a decreased expression of miR-145-5p was found in pemetrexed-resistant A400 cells than in A549 cells. Direct Sp1 targeting activity of miR-145-5p was demonstrated by a luciferase based Sp1 3′-UTR reporter. Changed expression of miR-145-5p in A400 or A549 cells by transfection of miR-145-5p mimic or inhibitor affected the sensitivity of the cells to pemetrexed. On the other hand, the overexpression of Sp1 in A549 cells caused the decreased sensitivity to pemetrexed, induced cell migratory capability, and epithelial–mesenchymal transition (EMT) related transcription factors such as Snail Family Transcriptional Repressor 1 and Zinc Finger E-Box Binding Homeobox 1. In addition, the overexpression of BMI1 in the A549 cells resulted in an increase in Sp1 and a decrease in miR-145-5p accompanied by the elevations of cell proliferation and EMT transcription factors, which could be reduced by the overexpression of miR-145-5p or by treatment with the Sp1 inhibitor of mithramycin A. In conclusion, the results of this study suggest that the downregulation of miR-145-5p by BMI1 overexpression could lead to the enhanced expression of Sp1 to induce the EMT process in pemetrexed-resistant NSCLC cells. These results suggest that increasing miR-145-5p expression by delivering RNA drugs may serve as a sensitizing agent for pemetrexed-resistant NSCLC patients.
MAPLE Processed Nanostructures for Antimicrobial Coatings
Authors: Hudiță Ariana , Grumezescu Valentina , Gherasim Oana , Grumezescu Alexandru Mihai , Dorcioman Gabriela , Negut Irina , Oprea Ovidiu-Cristian , Vasile Bogdan Ștefan , Gălățeanu Bianca , Curuțiu Carmen , Holban Alina Maria
Published: December 2022

Despite their great benefits for debilitated patients, indwelling devices are prone to become easily colonized by resident and opportunistic microorganisms, which have the ability to attach to their surfaces and form highly specialized communities called biofilms. These are extremely resistant to host defense mechanisms and antibiotics, leading to treatment failure and device replacement, but also to life-threatening complications. In this study, we aimed to optimize a silica (SiO2)-coated magnetite (Fe3O4)-based nanosystem containing the natural antimicrobial agent, eugenol (E), suitable for MAPLE (matrix-assisted pulsed laser evaporation) deposition as a bioactive coating for biomedical applications. X-ray diffraction, thermogravimetric analysis, Fourier-transform infrared spectroscopy, and transmission electron microscopy investigations were employed to characterize the obtained nanosystems. The in vitro tests evidenced the superior biocompatibility of such nanostructured coatings, as revealed by their non-cytotoxic activity and ability to promote cellular proliferation and sustain normal cellular development of dermal fibroblasts. Moreover, the obtained nanocoatings did not induce proinflammatory events in human blood samples. Our studies demonstrated that Fe3O4 NPs can improve the antimicrobial activity of E, while the use of a SiO2 matrix may increase its efficiency over prolonged periods of time. The Fe3O4@SiO2 nanosystems showed excellent biocompatibility, sustaining human dermal fibroblasts’ viability, proliferation, and typical architecture. More, the novel coatings lack proinflammatory potential as revealed by the absence of proinflammatory cytokine expression in response to human blood sample interactions.
Frontline Warrior microRNA167: A Battle of Survival
Authors: Suri Gurparsad Singh , Tiwari Manish
Published: December 2022

Plant pathogens such as viruses are detrimental to the survivorship of plant species. Coinfection of maize chlorotic mottle virus (MCMV) and the sugarcane mosaic virus (SCMV) causes a deadly disease in maize. An investigation by Liu et al. (2022) showed the role of Zma-miR167 in positively imparting resistance against the MCMV and SCMV. The authors identified ZmARF3 and ZmARF30 as the targets of Zma-miR167. ZmARF3 and ZmARF30 were identified as transcription factors that bind the cis-element in ZmPAO1 promoters to activate its expression. The authors showed how the Zma-miR167-ZmARF3/30-ZmPAO1 module functions differently in resistant and susceptible lines with high expression of Zma-miR167 in resistant lines correlated with the resistant phenotype. Finally, the authors concluded that MCMV-encoded p31 protein enhances ZmPAO1 enzyme activity for its survival in the host.
Rich CuO Nanowires Fabrication via Laser Post-Treatment of Laser-Textured Copper Substrate
Authors: Chen Zhekun , Yuan Gongfa , Zhou Rui , Huang Weipeng , Hong Minghui
Published: December 2022

The superhydrophobic and anti-icing properties of copper-based materials have been widely concerned. Laser texturing followed by thermal oxidation is a method to prepare durable CuO nanowires on Cu substrate. Rich CuO nanowires on micro-structures are required to meet its applications for anti-icing based on sustaining better Cassie–Baxter state stability. In this paper, dense CuO nanowires are obtained by additional laser post-treatment between the laser texturing and thermal oxidation. Uniform Cu2O and CuO layers form on the micro-structures by the laser post-treatment, which serves as the precursors for the growth of nanowires. The density of CuO nanowire increases from 44.3% to 61.4%, resulting in excellent superhydrophobicity and anti-icing performance. The delayed freezing time increases from 17 s to 1519 s at the temperature of −10 °C, which is more than 80 times longer compared to pure Cu substrate. The resulting anti-icing property shows durability through icing–melting cycles and taping tests. This feasible method opens new possibilities for enhanced anti-icing property on copper-based equipment used in low-temperature environments.
Spontaneous Release of Metalloradicals and Coordinatively Unsaturated Species in Asymmetric Iridium Dimers to Promote C-N Bond Formation
Authors: Chen Tsun-Ren , Chen Yi-Sheng , Li Chia-Ying , Lin Yen-Hsing , Chen Yu-Tung
Published: December 2022

An unusual iridium dimer 1, [(4-cpbo)Ir(μ-Cl)(μ-O)Ir(4-cpbo)] (4-cpbo = 4-chlorophenylbenzoxazolato-N,C2), was obtained by the oxidative addition of oxygen and reductive elimination of chlorine from a precursor [(4-cpbo)2Ir(μ-Cl)]2. This iridium dimer 1 has a metastable structure with an asymmetric bridge, can spontaneously release metalloradicals and coordinatively unsaturated species in solution at room temperature, and exhibits high catalytic ability for synthetic applications.
Regioselective One-Pot Synthesis of Novel Functionalized Organoselenium Compound by Bis-Alkoxyselenenylation of Alkenes with Selenium Dibromide and Alcohols
Authors: Potapov Vladimir A. , Musalov Maxim V. , Khabibulina Alfiya G. , Maylyan Arkady A. , Borodina Tatyana N. , Zinchenko Sergey V. , Amosova Svetlana V.
Published: December 2022

The one-pot efficient synthesis of novel functionalized organoselenium compound by bis-alkoxyselenenylation of alkenes with selenium dibromide and alcohols was developed. The reaction of the selenium dibromide with cyclopentene or cyclohexene in the system alcohol/sodium bicarbonate/methylene chloride at room temperature afforded bis(2-alkoxycycloalkyl) selenides in 90–99% yields. The regioselective and efficient method for bis-alkoxylation of terminal alkenes was developed based on the addition of selenium dibromide with 1-alkenes in acetonitrile followed by refluxing of addition products in alcohols in the presence of traces of sulfuric acid. This method made it possible to selectively obtain bis(2-alkoxyalkyl) selenides in 94–98% yields.
Dissolution Performance of Carbon/Hydroxyapatite Nanocomposite Prepared from Fish Scales
Authors: Sittitut Umaporn , Jettanasen Junya , Supothina Sitthisuntorn , Rattanakam Ramida
Published: December 2022

The utilization of waste as raw materials in the production of functional materials is a growing area of interest to promote global sustainable growth. This work studied the microstructure, chemical properties, and phosphorus (P) dissolution efficiency of carbon/hydroxyapatite (HAP) nanocomposites derived from fish scale wastes, namely FSBCs. Tilapia scales were pyrolyzed at different temperatures (450–600 °C) and the dissolution performance of the biochars in acidic media was evaluated. The pyrolysis of fish scales yielded biochars which are carbon/HAP nanocomposites of which the characteristics, including the specific surface area, carbon content, degree of crystallinity of HAP, and the degree of CO32− substitution in HAP crystals, were dependent on the pyrolysis temperature and had an influence on the dissolution efficiency. P dissolution experiments suggested that the specific surface area and the presence of carbon in the composite played an important role during the dissolution process. The presence of the organic residues on the carbon could potentially block the calcium sites on HAP from citric acid and lower the dissolution efficiency. The ratio of CO32−/PO43− substitution in HAP also affected the dissolubility of FSBCs. The manipulation of their surface area and the CO32−/PO43− ratio of these materials would allow us to increase its solubility, which could benefit a prospective application such as P fertilizer production.
Spider Assemblages of Tree Trunks and Tree Branches in Three Developmental Phases of Primeval Oak–Lime–Hornbeam Forest in the Białowieża National Park
Authors: Stańska Marzena , Stański Tomasz
Published: December 2022

The study was conducted in the Białowieża Forest, which is the only place in Europe where the full development cycle of forests takes place on a large scale. The objective of this study was to compare spider assemblages inhabiting tree trunks and tree branches in the optimal, terminal and regeneration phases of a primeval oak–lime–hornbeam stand, in terms of their abundance, species diversity and species richness. Spiders of tree branches were sampled using a sweep net into which branches were shaken, while spiders inhabiting tree trunks were collected using traps made of corrugated cardboard placed around the trunks. The three analysed phases did not differ in terms of total species richness. We found that the species diversity of both foliage-dwelling and trunk-dwelling spider assemblages was higher in the terminal phase compared to other phases, which may indicate that the former phase offered the most diverse niches for spiders as a result of the significant disturbance in the stand structure. In addition, we found fewer spider individuals and species in individual samples collected on tree branches from a plot in the regeneration phase compared to the other phases, which may be a consequence of the structure of the stand in this phase (low canopy cover, lush herbaceous vegetation).
Unraveling the Role of Lac Insects in Providing Natural Industrial Products
Authors: Bashir Nawaz Haider , Chen Huanhuan , Munir Shahzad , Wang Weiwei , Chen Hang , Sima Yong-Kang , An Jiandong
Published: December 2022

In the current era, products made from organic materials enjoy a privileged position because of their inherent safety. The eco-friendly properties of natural lac resins have increased their demand in many industries. It is secreted by sucking insects (Hemiptera, Kerriidae) and comprises three major components, viz., resin, dye, and wax. Lac insects are generally bivoltine in nature and are distributed in tropical and sub-tropical regions with complex multi-trophic habitats. Because of their sedentary habits, lac insects are more vulnerable to predators, parasitoids, squirrels, and rats, leading to a more than 50% reduction in production yield. To increase lac production, advanced-level molecular research is required to figure out the mechanism behind lac synthesis and secretion to improve lac yield and quality. The present review highlights metamorphosis, sexual dimorphism, multi-trophic habitat, host plants, and natural enemies of lac insects, lac composition, and applications, emphasizing the role of microbes, potential lac genes, and lac synthesis mechanisms in enhancing lac quality and production. The information provided here might be useful for lac researchers and for stakeholders aiming to make their products more eco-friendly.
Mechanics of the Prey Capture Technique of the South African Grassland Bolas Spider, Cladomelea akermani
Authors: Diaz Candido , Roff John
Published: December 2022

Spiders use various combinations of silks, adhesives, and behaviors to ensnare prey. One common but difficult-to-catch prey is moths. They easily escape typical orb-webs because their bodies are covered in tiny sacrificial scales that flake off when in contact with the web’s adhesives. This defense is defeated by spiders of the sub-family of Cyrtarachninae—moth-catching specialists who combine changes in orb-web structure, predatory behavior, and chemistry of the aggregate glue placed in those webs. The most extreme changes in web structure are shown by the bolas spiders which create only one or two glue droplets at the end of a single thread. They prey on male moths by releasing pheromones to draw them close. Here, we confirm the hypothesis that the spinning behavior of the spider is directly used to spin its glue droplets using a high-speed video camera to observe the captured behavior of the bolas spider Cladomelea akermani as it actively spins its body and bolas. We use the kinematics of the spider and bolas to begin to quantify and model the physical and mechanical properties of the bolas during prey capture. We then examine why this species chooses to spin its body, an energetically costly behavior, during prey capture. We test the hypothesis that spinning helps to spread pheromones by creating a computational fluid dynamics model of airflow within an open field and comparing it to that of airflow within a tree, a common environment for bolas spiders that do not spin. Spinning in an open environment creates turbulent air, spreading pheromones further and creating a pocket of pheromones. Conversely, spinning within a tree does little to affect the natural airflow.
Effects of PVY-Infected Tobacco Plants on the Adaptation of Myzus persicae (Hemiptera: Aphididae)
Authors: He Yingqin , Jiang Wenbin , Ding Wei , Chen Wenlong , Zhao Degang
Published: December 2022

The indirect interaction between viruses and their insect vectors via the host plants can mediate viral transmission. Thus, elucidating these tripartite interactions is crucial for controlling the occurrence of viral diseases. This study examined the feeding behavior and life table parameters of the green peach aphid, Myzus persicae, using electropenetrography and an age-stage, two-sex life table on PVY-infected and uninfected tobacco plants. Furthermore, the amino acid and soluble sugar contents in tobacco tissue at different stages of PVY infection were determined. The results showed that PVY-infected plants exerted remarkable effects on the feeding activities of M. persicae. Aphids exhibited a reduced non-probing duration and increased phloem sap ingestion on infected plants. Although the nymph development time on the PVY-infected plants was significantly shorter than that of uninfected plants, M. persicae reared on infected plants had reduced fecundity and significantly shortened adult longevity. On day 12, the sugar: amino acid ratio of the PVY-infected plants was significantly higher than that of uninfected plants, whereas the opposite was observed on day 24. Our results demonstrated that PVY could alter the adaptability of M. persicae by modifying the nutritional quality of tobacco plants. In addition, divergent effects on aphids were observed at different infection stages, which are crucial to consider while exploring the interactions between viruses, insect vectors, and host plants. These results provided significant information for comprehending PVY spread and outbreaks.
Effect of Leaf Trichomes in Different Species of Cucurbitaceae on Attachment Ability of the Melon Ladybird Beetle Chnootriba elaterii
Authors: Saitta Valerio , Rebora Manuela , Piersanti Silvana , Gorb Elena , Gorb Stanislav , Salerno Gianandrea
Published: December 2022

This study investigates the attachment ability of the oligophagous melon ladybird beetle Chnootriba elaterii to leaves of several Cucurbitaceae species. Using cryo-SEM, we described adult and larva tarsal attachment devices and leaf surface structures (glandular and non-glandular trichomes) in Citrullus lanatus, Cucumis melo, Cucumis sativus, Cucurbita moschata, Cucurbita pepo, Ecballium elaterium, Lagenaria siceraria and Luffa aegyptiaca. Using traction force experiments and centrifugal force tests, we measured the friction force exerted by females and larvae on plant leaves. We observed that Cucurbitaceae glandular trichomes do not affect insect attachment ability at both developmental stages, suggesting some adaptation of C. elaterii to its host plants, while non-glandular trichomes, when they are dense, short and flexible, heavily reduce the attachment ability of both insect stages. When trichomes are dense but stiff, only the larval force is reduced, probably because the larva has a single claw, in contrast to the adult having paired bifid dentate claws. The data on the mechanical interaction of C. elaterii at different developmental stages with different Cucurbitaceae species, combined with data on the chemical cues involved in the host plant selection, can help to unravel the complex factors driving the coevolution between an oligophagous insect and its host plant species.
SCADA System for Online Electrical Engineering Education
Authors: Alcayde Alfredo , Robalo Isabel , Montoya Francisco G. , Manzano-Agugliaro Francisco
Published: December 2022

Renewable energy sources are increasingly being integrated into small-scale production systems, so plants with multiple supply sources are becoming more common. This improvement in technology added to a greater social awareness of energy saving and resource usage, which makes flexible systems to manage these facilities necessary. Modern energy management can be more accessible to the interested public if it is located in universities, making it available to teachers and students alike. Furthermore, as it is a developing field of study, its location on campus facilitates research, maintenance, and financing. In this scenario, a SCADA system is proposed, capable of monitoring and centrally storing the values of the most important production and consumption parameters. In addition, by using this system, it is possible to control the state of the different energy sources in a centralized way, as well as their distribution in the plant where it is implemented. This study focuses on the management of a flexible, modern, and accessible solution to the advances in electrical systems because of technological development in this field, which broadens the experience of university teachers and students in their engineering careers. The systems have been put into practice in the facilities of a research and teaching laboratory at the University of Almeria, which integrates renewable and conventional energy resources.
Age-Related Mortality in STEMI Patients: Insight from One Year of HUB Centre Experience during the Pandemic
Authors: Tumminello Gabriele , D’Errico Andrea , Maruccio Alessio , Gentile Domitilla , Barbieri Lucia , Carugo Stefano
Published: December 2022

Background: Old patients have a poor prognosis when affected by ST elevation myocardial infarction (STEMI). The aim of our study was to evaluate the impact of age on acute and mid-term mortality in STEMI patients over one year in the pandemic period. Methods: we collected data on 283 STEMI patients divided into three groups according to age (not old, “Not-O”, ≤74 y/o; old, “O”, 75–84 y/o; very old, “Very-O”, ≥85 y/o). Results: the three groups did not differ in their clinical or procedural characteristics. The Very-O patients had a significantly increased incidence of in-hospital MACE (35%), mortality (30.0%), and percentage of cardiac death (25.0%). The only two independent predictors of in-hospital mortality were the ejection fraction (EF) [OR:0.902 (95% CI) 0.868–0.938; p < 0.0001] and COVID-19 infection [OR:3.177 (95% CI) 1.212–8.331; p = 0.019]. At follow-up (430 +/− days), the survival rates were decreased significatively among the age groups (Not-O 2.9% vs. O 14.8% vs. Very-O 28.6%; p < 0.0001), and the only two independent predictors of the follow-up mortality were the EF [OR:0.935 (95% CI) 0.891–0.982; p = 0.007] and age [OR:1.06 (95% CI) 1.018–1.110; p = 0.019]. Conclusions: in very old patients, all the accessory procedures that may be performed should be accurately and independently weighed up in terms of the risk–benefit balance and the real impact on the quality of life because of the poor mid-term prognosis.
A Prospective Randomized Study Comparing Functional Outcome in Distal Fibula Fractures between Conventional AO Semitubular Plating and Minimal Invasive Intramedullary “Photodynamic Bone Stabilisation”
Authors: Zyskowski Michael , Wurm Markus , Greve Frederik , Zehnder Philipp , Pflüger Patrick , Müller Michael , Biberthaler Peter , Kirchhoff Chlodwig
Published: December 2022

(1) Background: As age in western populations is rising, so too are fractures, e.g., of the distal fibula. The aim of this study was to find out whether a novel, minimally invasive intramedullary osteosynthesis technique for the treatment of distal fibula fractures in elderly patients results in not only a reduction of postoperative complications, but also a shorter hospitalization time, an improved clinical outcome, and preserved autonomy in geriatric trauma patients. (2) Methods: In this prospective study, the results following surgical treatment for distal fibula fractures in geriatric patients after using DePuy Synthes® one-third semitubular plate (Group I) or a minimally invasive intramedullary photodynamic Bone StabilizationSystem (IlluminOss®) (Group II) were compared at 6 weeks, 12 weeks, 6 months, and 1 year after initial treatment. (3) Results: Significant improvement regarding clinical outcome was shown in Group II 6 and 12 weeks after surgery. (4) Conclusions: Our study results demonstrate that the use of this new intramedullary stabilization system in combination with an immediate postoperative weight bearing seems to be a safe and stable treatment option for ankle fractures in geriatric patients, especially in the early stages of recovery.
Patient-Reported Outcome on Quality of Life and Pain after Revision Arthroplasty for Periprosthetic Joint Infection: A Cross-Sectional Study
Authors: Zhang Chaofan , Liu Ziyu , Lin Yunzhi , Cai Yuanqing , Zhang Xuehui , Huang Zida , Huang Ying , Li Wenbo , Fang Xinyu , Zhang Wenming
Published: December 2022

This study aims to explore the quality of life (QOL) and pain after revision surgery for periprosthetic joint infection (PJI) based on patients’ reported outcomes. A cross-sectional questionnaire survey was conducted and 137 valid responses were included (response rate 64.0%). A total of 42 patients underwent debridement with implant retention (DAIR), 31 underwent one-stage revision, and 64 underwent two-stage revision. The average overall SF-36 score was 70.3. The DAIR group had significantly higher SF-36 than the two-stage revision group (p = 0.01). There was no significant difference between the one-stage revision group and the other two groups. A total of 74.5% of patients reported pain with an average McGill Pain Questionnaire (MPQ) score of 8.6. There was no significant difference in the MPQ scores among the three groups. Simple linear regression analyses demonstrated that higher preoperative PMN%, VAS, and shorter hospital stay were associated with pain (adjusted R2 = 4%, p = 0.020; adjusted R2 = 2.1%, p = 0.048; adjusted R2 = 2.1%, p = 0.049; respectively). We concluded that the overall QOL of patients after revision surgery for PJI is generally satisfactory. Persistent pain is prevalent, but the severity was mostly mild. Preoperative PMN%, VAS, and hospital stay were associated with postoperative pain.
Effects of Neoadjuvant Radiotherapy on Survival in Patients with Stage IIIA-N2 Non-Small-Cell Lung Cancer Following Pneumonectomy
Authors: Qu Chenghao , Li Rongyang , Han Jingyi , Yue Weiming , Tian Hui
Published: December 2022

Background: Pneumonectomy is a drastic but sometimes inevitable treatment option for patients with non-small-cell lung cancer (NSCLC) to improve their chances for long-term survival. However, the optimal adjuvant radiotherapy used for patients with N2 NSCLC following pneumonectomy remains unclear in the literature. Methods: T1-4N0-2M0 NSCLC patients registered in the Surveillance, Epidemiology, and End Results database were retrospectively analyzed. Propensity score matching was applied to balance the assignment of patients. Cox proportional hazards models and Kaplan–Meier analyses were used to identify the factors related to overall survival rates. Restricted cubic splines were used to detect the possible nonlinear dependency of the relationship between the risk of survival and age. Results: A total of 4308 NSCLC patients were enrolled in this study. In N2 patients, the long-term outcome of the chemotherapy and postoperative radiotherapy groups was the worst (p = 0.014). Subgroup analyses showed that the influence of age on survival outcome was confined to patients who received chemotherapy and neoadjuvant radiotherapy (p = 0.004). Meanwhile, patients >65 years of age who received chemotherapy and neoadjuvant radiotherapy had significantly worse prognoses than those in the chemotherapy group (p = 0.005). Conclusions: Our results show that neoadjuvant radiotherapy may have potential benefits in patients aged ≤ 65 years who are scheduled for pneumonectomy, but not in elderly patients.
Acute Onset Quadriplegia and Stroke: Look at the Brainstem, Look at the Midline
Authors: Zedde Marialuisa , Grisendi Ilaria , Pezzella Francesca Romana , Napoli Manuela , Moratti Claudio , Valzania Franco , Pascarella Rosario
Published: December 2022

Acute onset quadriplegia with or without facial sparing is an extremely rare vascular syndrome, and the main focus of attention is on the cervical and upper thoracic spinal cord as the putative site of the damage. Quadriplegia has been occasionally reported in brainstem strokes within well-defined lesion patterns, but these reports have gained little attention so far because of the rarity of this clinical syndrome. The clinical, neuroanatomical and neuroimaging features of ischemic stroke locations associated with quadriplegia have been collected and reviewed in a pragmatical view, which includes a detailed description of the neurological signs associated with the damage of the pyramidal pathways. Two clinical examples have been added to raise practical suggestions in neurovascular practice. Ischemic stroke sites determining quadriplegia have some main well-defined midline locations in the brainstem, involving the pyramidal pathways of both sides in a single synchronous ischemic lesion in the medulla oblongata and in the pons. Several accompanying neurological signs have been described when the ischemic lesion involves tracts and nuclei other than the pyramidal pathways, and they can be useful as localizing clues. In some cases, the typical neuroimaging appearance of the ischemic lesion on Magnetic Resonance Imaging (MRI) has been reported as being a “heart appearance sign”. This last sign has been described in midbrain strokes too, but this location is not associated with quadriplegia. The main etiology is atherothrombosis involving the intradural segment of the vertebral artery (VA) and their perforating branches. Two clinical examples of these rare vascular syndromes have been chosen to support a pragmatical discussion about the management of these cases. A midline ischemic stroke in the brainstem is a very rare vascular syndrome, and the acute onset quadriplegia is a distinctive feature of it. The awareness of this cerebrovascular manifestation might help to recognize and treat these patients.
Psychiatric Symptoms in Patients with Cerebral Endometriosis: A Case Report and Literature Review
Authors: Elefante Camilla , Brancati Giulio Emilio , Oragvelidze Elene , Lattanzi Lorenzo , Maremmani Icro , Perugi Giulio
Published: December 2022

Endometriosis is a systemic medical condition characterized by endometrial tissue that is abnormally implanted in extrauterine sites, including the central nervous system. In this article, we reported the case of a patient with presumed cerebral endometriosis who was diagnosed with bipolar disorder and panic disorder and systematically reviewed the literature for previously reported neuropsychiatric symptoms in patients with cerebral and cerebellar endometriosis. The PubMed, Scopus, and Web of Science bibliographic databases were searched according to the PRISMA guidelines. Seven previous case reports were found and described. While neurological disturbances dominated the clinical picture in the cases retrieved from the literature, our patient represented the first case to show both neurological and psychiatric manifestations. Atypical features of bipolar disorder including chronic mood instability, mixed episodes, and excitatory interepisodic symptoms were highlighted. During the neuropsychological evaluation, a dysexecutive profile consistent with frontal lobe pathology was evidenced. We hypothesized that the course and features of the illness were largely influenced by the presence of documented brain lesions compatible with endometrial implants, especially in the frontal region. Accordingly, patients with endometriosis who exhibit neurological as well as mental symptoms should be investigated for cerebral lesions.
Multidisciplinary Postoperative Validation of 18F-FDG PET/CT Scan in Nodal Staging of Resected Non-Small Cell Lung Cancer
Authors: Bedetti Benedetta , Schnorr Philipp , May Sarah , Ruhlmann Jürgen , Ahmadzadehfar Hojjat , Essler Markus , Quaas Alexander , Büttner Reinhard , Schmidt Joachim , Palmedo Holger , Ko Yon-Dschun , Wilhelm Kai
Published: December 2022

Background: The aim of this study was to examine the validity of PET/CT scans in the preoperative identification of lymph node metastases (LNM) and compare them with postoperative outcomes. Methods: In this retrospective study, we included 87 patients with a solitary lung nodule or biopsy-proven non-small cell lung cancer treated in our institution from 2009 to 2015. Patients were divided into two groups and four subgroups, depending on pre- and postoperative findings. Results: According to our analysis, PET/CT scan has a sensitivity of 50%, a specificity of 88.89%, a positive predictive value of 63.16%, and a negative predictive value of 82.35%. Among the patients, 13.8% were downstaged in PET-CT, while 8% were upstaged. In 78.2% of cases, the PET/CT evaluation was consistent with the histology. Metastases without extracapsular invasion were seldom recognized on PET/CT. Conclusions: This analysis showed the significance of extracapsular tumor invasion, which causes an inflammatory reaction, on LNM, which is probably responsible for preoperative false-positive findings. In conclusion, PET/CT scans are very effective in identifying patients without tumors. Furthermore, it is highly probable that patients with negative findings are free of disease.
Molecular Mechanism of Curcumin Derivative on YAP Pathway against Ovarian Cancer
Authors: Zheng Nan , Liu Shan , Zeng Huiting , Zhao Huajun , Jin Lixu
Published: December 2022

The purpose of this study is to study the effect of curcumin derivative WZ10 on the proliferation, invasion and apoptosis of ovarian cancer OVCAR3 cells, and to explore its mechanism. MTT assay was used to detect the effect of WZ10 on the proliferation of ovarian cancer OVCAR3 cells; Annexin V/PI double staining flow cytometry was used to detect the effect of WZ10 on cell apoptosis; Transwell method was used to detect the effect of WZ10 on cell invasiveness; Western blot was used to investigate the effect of WZ10 Mechanisms affecting OVCAR3 activity in ovarian cancer in vitro. Our results show that WZ10 treatment could significantly inhibit the proliferation and invasion of OVCAR3 cells, induce apoptosis of OVCAR3 cells in a dose-dependent manner. After knockdown of Hippo expression with sh-RNA, further combined treatment with WZ10 had no significant image on ovarian cancer OVCAR3 cells. In conclusion: WZ10 can significantly inhibit the proliferation of OVCAR3 cells, reduce cell invasion and proliferation by downregulating the activation of Hippo-YAP pathway, and induce cell apoptosis.
Screening and Identification of Key Biomarkers in Metastatic Uveal Melanoma: Evidence from a Bioinformatic Analysis
Authors: Wang Tan , Wang Zixing , Yang Jingyuan , Chen Youxin , Min Hanyi
Published: December 2022

Purpose: To identify key biomarkers in the metastasis of uveal melanoma (UM). Methods: The microarray datasets GSE27831 and GSE22138 were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were identified, and functional enrichment analyses were performed. A protein–protein interaction network was constructed, and four algorithms were performed to increase the reliability of hub genes. Biomarker analysis and metastasis-free survival analysis were performed to screen and verify prognostic hub genes. Results: A total of 138 DEGs were identified, consisting of 71 downregulated genes and 67 upregulated genes. Four genes (ROBO1, FMN1, FYN and FXR1) were selected as hub genes. Biomarker analysis and metastasis-free survival analysis showed that ROBO1, FMN1, FYN and FXR1 were factors affecting the metastasis and metastasis-free survival of UM (all p < 0.05). High expression of ROBO1 and low expression of FMN1 were associated with longer metastasis-free survival. Multivariable logistic regression and Cox analyses in GSE 27831 indicated that ROBO1 was an independent factor affecting metastasis and metastasis-free survival of UM (p = 0.010 and p = 0.009), while ROBO1 and FMN1 were independent factors affecting metastasis and metastasis-free survival of UM in GSE22138 (all p < 0.05). Conclusions: ROBO1, FMN1, FYN and FXR1 should be regarded as diagnostic biomarkers for the metastasis of UM, especially ROBO1 and FMN1. High expression of ROBO1 and low expression of FMN1 were associated with longer metastasis-free survival. This study may facilitate the understanding of the molecular mechanisms underlying the metastasis of UM.
Multi-Hole Drilling Tool Path Planning and Cost Management through Hybrid SFLA-ACO Algorithm for Composites and Hybrid Materials
Authors: Mehmood Nasir , Umer Muhammad , Asgher Umer
Published: December 2022

In the process of drilling multiple holes in composites and hybrid materials, almost 70% of the time is consumed in tool traveling and tool changing. Recently, researchers have focused on this consumption of time for optimization of the tool path. A literature review revealed the following research gap: little work has been performed on the hybridization of metaheuristics. In the present study, the hybridization of SFLA and ACO metaheuristic algorithms is carried out, which is based on this research gap. The hybridization of SFLA and ACO metaheuristic algorithms provides originality and novelty in this study. The main objective of this study is to minimize the tool path in drilling problems. The proposed algorithm was applied to five benchmark multi-hole drilling problems and one industrial problem from the literature. The outcome of this work is evaluated with the results of dynamic programming (DP), ACO, an immune-based evolutionary approach (IA), and a modified SFLA for five benchmark problems. The accuracy of the results was improved by 2.27% using the proposed hybrid algorithm, indicating that the proposed algorithm is superior to DP, ACO, IA, and the modified SFLA. Additionally, the results of the proposed hybrid algorithm for an example industrial problem from the literature were compared with those of the SFLA and modified SFLA. The proposed algorithm reduced the total cost by 6.17% and 3.76% compared with the SFLA and modified SFLA, respectively. Thus, the efficacy of the proposed hybrid algorithm was confirmed, along with its applicability.
Development of Prediction Models for the Torsion Capacity of Reinforced Concrete Beams Using M5P and Nonlinear Regression Models
Authors: Henedy Sadiq N. , Naser Ali H. , Imran Hamza , Bernardo Luís F. A. , Teixeira Mafalda M. , Al-Khafaji Zainab
Published: December 2022

Torsional strength is related with one of the most critical failure types for the design and assessment of reinforced concrete (RC) members due to the complexity of the associated stress state and low ductility. Previous studies have shown that reliable methods to predict the torsional strength of RC beams are still needed, namely for over-reinforced and high-strength RC beams. This research aims to offer a novel set of models to predict the torsional strength of RC beams with a wide range of design attributes and geometries by using advanced M5P tree and nonlinear regression models. For this, a broad database with 202 experimental tests is used to generate highly reliable and resilient models. To build the models, three independent variables related with the properties of the RC beams are considered: concrete cross-section area (area enclosed within the outer perimeter of the cross-section), concrete compressive strength, and torsional reinforcement factor (which accounts for the type—longitudinal or transverse—amount, and yielding strength of the torsional reinforcement). In contrast to multiple nonlinear regression approaches, the findings show that the M5P tree approach has the best estimation in terms of both accuracy and safety. Furthermore, M5P model predictions are far more accurate and safer than the most prevalent design equations. Finally, sensitivity and parametric studies are used to confirm the robustness of the presented models.
Developments and Industrial Applications of Basalt Fibre Reinforced Composite Materials
Authors: Chowdhury Indraneel R. , Pemberton Richard , Summerscales John
Published: December 2022

Basalt mineral fibre, made directly from basalt rock, has good mechanical behavior, superior thermal stability, better chemical durability, good moisture resistance and can easily be recycled when compared to E-glass fibres (borosilicate glass is called ‘E-glass’ or ‘electric al-grade glass’ because of its high electrical resistance) which are traditionally used in structural composites for industrial applications. Industrial adoption of basalt fibre reinforced composites (FRC) is still very low mainly due to inadequate data and lower production volumes leading to higher cost. These reasons constrain the composites industry from seriously considering basalt as a potential alternative to conventional (e.g., E-glass) fibre reinforced composites for different applications. This paper provides a critical review of the state-of-the-art concerning basalt FRC highlighting the increasing trend in research and publications related to basalt composites. The paper also provides information regarding physico-chemical, and mechanical properties of basalt fibres, some initial Life cycle assessment inventory data is also included, and reviews common industrial applications of basalt fibre composites.
Dielectric Spectroscopy of Melt-Mixed Polypropylene and Pyrolytically Stripped Carbon Nanofiber Composites
Authors: Samir Zineb , Paleo Antonio J. , Aribou Najoia , Nioua Yassine , da Silva Jaime Oliveira , Cerqueira Maria F. , Moreira Joaquim Agostinho , Achour Mohammed E.
Published: December 2022

In this work, pyrolytically stripped carbon nanofiber (CNF) polypropylene (PP) composites were synthesized following a scalable melt-mixing method, and the effects of CNF weight concentrations on the electrical conductivity, dielectric permittivity, electrical modulus and electrical impedance of PP/CNF composites were studied. Quite unexpectedly, the electrical conductivity of PP/CNF composites improved only slightly as the incorporation of CNFs was raised, yielding a maximum of ~10−10 S m−1 for PP/CNF 5 wt. % composites. The increase corresponded to a gradual improvement of the dielectric constant up to a maximum of ~9 for PP/CNF 5 wt. % composites at 1 MHz, which was attributed to the raise of interface polarization effect. Moreover, the Cole–Cole model was used to analyze the effects of CNF concentrations on the dielectric relaxation of PP/CNF composites, from which was deduced that the incorporation of CNFs increases their dielectric strength and relaxation times. The analysis gathered here aims to provide a better insight into the enhanced dielectric properties observed in low-conducting polymer composites filled with CNFs.
Natural Rubber Composites Reinforced with Green Silica from Rice Husk: Effect of Filler Loading on Mechanical Properties
Authors: Choophun Nicha , Chaiammart Nattapat , Sukthavon Kantavee , Veranitisagul Chatchai , Laobuthee Apirat , Watthanaphanit Anyarat , Panomsuwan Gasidit
Published: December 2022

Natural rubber (NR) composites filled with silica are typically used for tire tread applications owing to their low energy consumption and low rolling resistance. Tire tread properties vary broadly depending on the compound formulation and curing conditions. Silica loading is recognized as a critical factor influencing the mechanical properties of the composites. In this work, we aim to investigate the effect of silica loading (10–50 phr) on the mechanical properties of NR composites. Silica was prepared from rice husk waste via chemical treatment and subsequent calcination at 600 °C. Prior to the compound mixing process, silica was modified by a silane coupling agent to improve compatibility with the NR matrix. The NR compounds reinforced with silane-modified silica from rice husk were prepared using a two-roll mill machine. The scorch and cure times increased as the silica loading increased. The mechanical properties of the NR composites, including tensile strength, elongation at break, modulus, hardness, and abrasion loss, were examined as a function of silica loading. Tensile strength increased and reached the maximum value at 20 phr but decreased at high loading owing to the agglomeration of silica in the NR matrix. With increasing silica loading, hardness and modulus increased, whereas elongation at break and abrasion resistance decreased slightly. These results indicate that NR composites filled with silica are stiffer and harder at a higher silica loading due to the strong interaction between silica and the NR matrix, inhibiting the segmental mobility of rubber chains. We anticipate that the compound formulation presented in this work could potentially be adapted to tire tread applications.