bluuebunny/crossref_metadata_2025 huggingface dataset:
Common trajectories of highly effective CD19-specific CAR T cells identified by endogenous T cell receptor lineages
Authors: Wilson Taylor L. , Kim Hyunjin , Chou Ching-Heng , Langfitt Deanna , Allen E. Kaitlynn , Métais Jean-Yves , Pogorelyy Mikhail V. , Kottapalli Pratibha , Trivedi Sanchit , Olsen Scott R. , Lockey Timothy , Willis Catherine , Meagher Michael M. , Triplett Brandon M. , Talleur Aimee C. , Gottschalk Stephen , Crawford Jeremy Chase , Thomas Paul G.
Published: December 2021

Current chimeric antigen receptor-modified (CAR) T cell therapy products are evaluated in bulk, without assessment of the possible heterogeneity in effector potential between cells. Conceivably, only a subset of the pre-infusion product differentiates into optimal effectors. We generated a comprehensive single-cell gene expression and T cell receptor (TCR) sequencing dataset using both pre- and post-infusion CD19-CAR T cells from peripheral blood and bone marrow of pediatric patients with B cell acute lymphoblastic leukemia (B-ALL). We identified potent effector post-infusion cells with identical TCRs to a subset of pre-infusion CAR T cells. Effector precursor CAR T cells exhibited a unique transcriptional profile compared to other pre-infusion cells, and the number of effector precursor cells infused correlated with peak CAR T cell expansion. Additionally, we identified an unexpected cell surface phenotype (TIGIT+, CD62Llo, CD27-), conventionally associated with inhibiting effective T cell responses, that we used to successfully enrich for subsequent effector potential. Collectively, these results demonstrate that highly diverse effector potentials are present among cells in pre-infusion cell products, which can be exploited for diagnostic and therapeutic applications. Furthermore, we provide an integrative experimental and analytical framework for elucidating the biological mechanisms underlying effector development in other CAR T cell therapy products.
Detection of brain somatic variation in epilepsy-associated developmental lesions
Authors: Bedrosian Tracy A. , Miller Katherine E. , Grischow Olivia E. , Yoon Hyojung , Schieffer Kathleen M. , LaHaye Stephanie , Miller Anthony R. , Navarro Jason , Westfall Jesse , Leraas Kristen , Choi Samantha , Williamson Rachel , Fitch James , Lee Kristy , McGrath Sean , Cottrell Catherine E. , Magrini Vincent , Leonard Jeffrey , Pindrik Jonathan , Shaikhouni Ammar , Boué Daniel R. , Thomas Diana L. , Pierson Christopher R. , Wilson Richard K. , Ostendorf Adam P. , Mardis Elaine R. , Koboldt Daniel C.
Published: December 2021

Epilepsy-associated developmental lesions, including malformations of cortical development and low-grade developmental tumors, represent a major cause of drug-resistant seizures requiring surgical intervention in children. Brain-restricted somatic mosaicism has been implicated in the genetic etiology of these lesions; however, many contributory genes remain unidentified. We enrolled 50 children undergoing epilepsy surgery into a translational research study. We performed exome and RNA-sequencing of resected brain tissue samples to identify somatic variation. We uncovered candidate disease-causing somatic variation affecting 28 patients (56%), as well as candidate germline variants affecting 4 patients (8%). We confirmed somatic findings using high-depth targeted DNA sequencing. In agreement with previous studies, we identified somatic variation affecting SLC35A2 and MTOR pathway genes in patients with focal cortical dysplasia. Somatic gains of chromosome 1q were detected in 30% (3 of 10) Type I FCD patients. Somatic variation of MAPK pathway genes (i.e., FGFR1, FGFR2, BRAF, KRAS) was associated with low-grade epilepsy-associated developmental tumors. Somatic structural variation accounted for over one-half of epilepsy-associated tumor diagnoses. Sampling across multiple anatomic regions revealed that somatic variant allele fractions vary widely within epileptogenic tissue. Finally, we identified putative disease-causing variants in genes (EEF2, NAV2, PTPN11) not yet associated with focal cortical dysplasia. These results further elucidate the genetic basis of structural brain abnormalities leading to focal epilepsy in children and point to new candidate disease genes.
Study Design: Validation of clinical acceptability of deep-learning-based automated segmentation of organs-at-risk for head-and-neck radiotherapy treatment planning
Authors: Anand Aman , Beltran Chris J. , Brooke Mark D. , Buroker Justine R. , DeWees Todd A. , Foote Robert L. , Foss Olivia R. , Hughes Cían O. , Hunzeker Ashley E. , Lucido J. John , Morigami Megumi , Moseley Douglas J. , Pafundi Deanna H. , Patel Samir H. , Patel Yojan , Ridgway Ana K. , Tryggestad Erik J. , Wilson Megan Z. , Xi Lihong , Zverovitch Alexei
Published: December 2021

This document reports the design of a retrospective study to validate the clinical acceptability of a deep-learning-based model for the autosegmentation of organs-at-risk (OARs) for use in radiotherapy treatment planning for head & neck (H&N) cancer patients.
Reduced Neutralization of SARS-CoV-2 Omicron Variant by Vaccine Sera and Monoclonal Antibodies
Authors: Wilhelm Alexander , Widera Marek , Grikscheit Katharina , Toptan Tuna , Schenk Barbara , Pallas Christiane , Metzler Melinda , Kohmer Niko , Hoehl Sebastian , Helfritz Fabian A. , Wolf Timo , Goetsch Udo , Ciesek Sandra
Published: December 2021

Due to numerous mutations in the spike protein, the SARS-CoV-2 variant of concern Omicron (B.1.1.529) raises serious concerns since it may significantly limit the antibody-mediated neutralization and increase the risk of reinfections. While a rapid increase in the number of cases is being reported worldwide, until now there has been uncertainty about the efficacy of vaccinations and monoclonal antibodies. Our in vitro findings using authentic SARS-CoV-2 variants indicate that in contrast to the currently circulating Delta variant, the neutralization efficacy of vaccine-elicited sera against Omicron was severely reduced highlighting T-cell mediated immunity as essential barrier to prevent severe COVID-19. Since SARS-CoV-2 Omicron was resistant to casirivimab and imdevimab, genotyping of SARS-CoV-2 may be needed before initiating mAb treatment. Variant-specific vaccines and mAb agents may be required to treat COVID-19 due to Omicron and other emerging variants of concern.
Fiber orientation downsampling compromises the computation of white matter tract-related deformation
Authors: Zhou Zhou , Wang Teng , Jörgens Daniel , Li Xiaogai
Published: December 2021

Incorporating neuroimaging-revealed structural details into finite element (FE) head models opens vast new opportunities to better understand brain injury mechanisms. Recently, growing efforts have been made to integrate fiber orientation from diffusion tensor imaging (DTI) into FE models to predict white matter (WM) tract-related deformation that is biomechanically characterized by tract-related strains. Commonly used approaches often downsample the spatially enriched fiber orientation to match the FE resolution with one orientation per element (i.e., element-wise orientation implementation). However, the validity of such downsampling operation and corresponding influences on the computed tract-related strains remain elusive. To address this, the current study proposed a new approach to integrate voxel-wise fiber orientation from one DTI atlas (isotropic resolution of 1 mm3) into FE models by embedding orientations from multiple voxels within one element (i.e., voxel-wise orientation implementation). By setting the responses revealed by the newly proposed voxel-wise orientation implementation as the reference, we evaluated the reliability of two previous downsampling approaches by examining the downsampled fiber orientation and the computationally predicted tract-related strains secondary to one concussive impact. Two FE models with varying element sizes (i.e., 6.37 ± 1.60 mm and 1.28 ± 0.55 mm, respectively) were incorporated. The results showed that, for the model with a large voxel-mesh resolution mismatch, the downsampled element-wise fiber orientation, with respect to its voxel-wise counterpart, exhibited an absolute deviation over 30° across the WM/gray matter interface and the pons regions. Accordingly, this orientation deviation compromised the computation of tract-related strains with normalized root-mean-square errors up to 30% and underestimated the peak tract-related strains up to 10%. For the other FE model with finer meshes, the downsampling-induced effects were lower, both on the fiber orientation and tract-related strains. Taken together, the voxel-wise orientation implementation is recommended in future studies as it leverages the DTI-delineated fiber orientation to a larger extent than the element-wise orientation implementation. Thus, this study yields novel insights on integrating neuroimaging-revealed fiber orientation into FE models and may better inform the computation of WM tract-related deformation, which are crucial for advancing the etiological understanding and computational predictability of brain injury.

Graphic abstract
Mechanical ventilation affects the microecology of the rat respiratory tract
Authors: Xue-Meng Chen , Gao-Wang Liu , Xiao-Mei Ling , Fan-Fang Zeng , Jin-Fang Xiao
Published: December 2021

Background

The most common ‘second strike’ in mechanically ventilated patients is a pulmonary infection caused by the ease with which bacteria can invade and colonize the lungs due to mechanical ventilation. At the same time, metastasis of lower airway microbiota may have significant implications in the development of intubation mechanical ventilation lung inflammation. Thus, we establish a rat model of tracheal intubation with mechanical ventilation and explore the effects of mechanical ventilation on lung injury and microbiological changes in rats.

Methods

Sprague-Dawley rats were randomized into control, Spontaneously Breathing (1, 3, 6 hours), Mechanical ventilation(1, 3, 6 hours) groups. Lung wet to dry weight ratio (W/D weight ratio) and Lung histopathological injury score were evaluated.16SrDNA sequencing was performed to explore respiratory flora changes.

Results

Bacterial diversity was comparable between healthy and intubation mechanical ventilation rats, with time relation. Ordination analyses revealed that samples clustered more dispersing by tracheal intubation and mechanical ventilation. Finally, predicted metagenomes suggested a substantial increase in biofilm formation phenotype during early tracheal intubation and mechanical ventilation.

Conclusion

Collectively, these results establish a link between the duration of mechanical ventilation and alterations to the respiratory tract microecology. In future studies, we hope to discover the effectiveness of new immunomodulatory or probiotic bacteria to prevent airway diseases associated with ventilator therapy.
Dual engagement of the nucleosomal acidic patches is essential for deposition of histone H2A.Z by SWR1C
Authors: Baier Alexander S. , Gioacchini Nathan , Eek Priit , Leith Erik M. , Tan Song , Peterson Craig L.
Published: December 2021

The SWR1C chromatin remodeling enzyme catalyzes the ATP-dependent exchange of nucleosomal histone H2A for the histone variant H2A.Z, a key variant involved in a multitude of nuclear functions. How the 14-subunit SWR1C engages the nucleosomal substrate remains largely unknown. Numerous studies on the ISWI, CHD1, and SWI/SNF families of chromatin remodeling enzymes have demonstrated key roles for the nucleosomal acidic patch for remodeling activity, however a role for this nucleosomal epitope in nucleosome editing by SWR1C has not been tested. Here, we employ a variety of biochemical assays to demonstrate an essential role for the acidic patch in the H2A.Z exchange reaction. Utilizing asymmetrically assembled nucleosomes, we demonstrate that the acidic patches on each face of the nucleosome are required for SWR1C-mediated dimer exchange, suggesting SWR1C engages the nucleosome in a “pincer-like” conformation, engaging both patches simultaneously. Loss of a single acidic patch results in loss of high affinity nucleosome binding and nucleosomal stimulation of ATPase activity. We identify a conserved arginine-rich motif within the Swc5 subunit that binds the acidic patch and is key for dimer exchange activity. In addition, our cryoEM structure of a Swc5-nucleosome complex suggests that promoter proximal, histone H2B ubiquitinylation may regulate H2A.Z deposition. Together these findings provide new insights into how SWR1C engages its nucleosomal substrate to promote efficient H2A.Z deposition.
Chlorine redox chemistry is not rare in biology
Authors: Barnum Tyler P. , Coates John D.
Published: December 2021

Chlorine is abundant in cells and biomolecules, yet the biology of chlorine oxidation and reduction is poorly understood. Some bacteria encode the enzyme chlorite dismutase (Cld), which detoxifies chlorite (CIO2-) by converting it to chloride (Cl-) and molecular oxygen (O2). Cld is highly specific for chlorite and aside from low hydrogen peroxide activity has no known alternative substrate. Here, we reasoned that because chlorite is an intermediate oxidation state of chlorine, Cld can be used as a biomarker for oxidized chlorine species in microorganisms and microbial habitats. Cld was abundant in metagenomes from soils and freshwater to water treatment systems. About 5% of bacterial and archaeal genera contain an organism encoding Cld in its genome, and within some genera Cld is nearly conserved. Cld has been subjected to extensive horizontal gene transfer, suggesting selection by chlorite is episodic yet strong. Cld was also used as a biomarker to predict genes related to chlorine redox chemistry. Genes found to have a genetic association with Cld include known genes for responding to reactive chlorine species and uncharacterized genes for transporters, regulatory elements, and putative oxidoreductases that present targets for future research. Cld was repeatedly co-located in genomes with genes for enzymes that can inadvertently reduce perchlorate (CIO4-) or chlorate (CIO3-), confirming that in nature (per)chlorate reduction does not only occur in specialized anaerobic respiratory metabolisms. The presence of Cld in genomes of obligate aerobes without such enzymes suggested that chlorite, like hypochlorous acid (HOCl), might be formed by oxidative processes within natural habitats. In summary, the comparative genomics of Cld has provided an atlas for a deeper understanding of chlorine oxidation and reduction reactions that are an underrecognized feature of biology.
Cerebrofacial vascular metameric syndrome is caused by somatic pathogenic variants in PIK3CA
Authors: Sheppard Sarah E. , Sanders Victoria R. , Srinivasan Abhay , Finn Laura S. , Adams Denise , Elton Andrew , Amlie-Lefond Catherine , Nelson Zoe , Dmyterko Victoria , Jensen Dana , Zenner Kaitlyn , Perkins Jonathan , Bennett James T.
Published: December 2021

Disorganized morphogenesis of arteries, veins, capillaries, and lymphatic vessels results in vascular malformations. Most individuals with isolated vascular malformations have postzygotic (mosaic), activating pathogenic variants in a handful of oncogenes within the PI3K–RAS–MAPK pathway (Padia et al., Laryngoscope Investig Otolaryngol 4: 170–173 [2019]). Activating pathogenic variants in the gene PIK3CA, which encodes for the catalytic subunit of phosphatidylinositol 3-kinase, are present in both lymphatic and venous malformations as well as arteriovenous malformations in other complex disorders such as CLOVES syndrome (congenital, lipomatous, overgrowth, vascular malformations, epidermal anevi, scoliosis) (Luks et al., Pediatr Dev Pathol 16: 51 [2013]; Luks et al., J Pediatr 166: 1048–1054.e1–5 [2015]; Al-Olabi et al., J Clin Invest 128: 1496–1508 [2018]). These vascular malformations are part of the PIK3CA-related overgrowth spectrum, a spectrum of entities that have regionalized disordered growth due to the presence of tissue-restricted postzygotic PIK3CA pathogenic variants (Keppler-Noreuil et al., Am J Med Genet A 167A: 287–295 [2015]). Cerebrofacial vascular metameric syndrome (CVMS; also described as cerebrofacial arteriovenous metameric syndrome, Bonnet–Dechaume–Blanc syndrome, and Wyburn–Mason syndrome) is the association of retinal, facial, and cerebral vascular malformations (Bhattacharya et al., Interv Neuroradiol 7: 5–17 [2001]; Krings et al., Neuroimaging Clin N Am 17: 245–258 [2007]). The segmental distribution, the presence of tissue overgrowth, and the absence of familial recurrence are all consistent with CVMS being caused by a postzygotic mutation, which has been hypothesized by previous authors (Brinjiki et al., Am J Neuroradiol 39: 2103–2107 [2018]). However, the genetic cause of CVMS has not yet been described. Here, we present three individuals with CVMS and mosaic activating pathogenic variants within the gene PIK3CA. We propose that CVMS be recognized as part of the PIK3CA-related overgrowth spectrum, providing justification for future trials using pharmacologic PIK3CA inhibitors (e.g., alpelisib) for these difficult-to-treat patients.
The paradoxical consequences of leader feedback seeking for subordinates: a theoretical framework
Authors: Carvalho Sheldon , Carvalho Fallan Kirby , Carvalho Charles
Published: December 2021

Purpose

Scholars in the feedback seeking domain have predominantly focused on subordinate feedback seeking. The authors still know very little about feedback seeking when the leader is the “seeker” and subordinates are the “targets” of such seeking. This paper aims to develop a theoretical framework that explores the potential benefits and costs of leader feedback seeking, specifically, leader feedback inquiry for subordinates.

Design/methodology/approach

The authors draw upon the transactional theory of stress to propose a framework in which leader feedback inquiry influences two subordinate behaviors (in-role and proactive skill development behaviors) via appraisal processes (challenge and threat appraisals). With insights from regulatory focus theory, the authors propose that individual characteristics, namely, the regulatory focus of subordinates (promotion and prevention focus), determine the appraisals of leader feedback inquiry, subsequently influencing subordinate behavioral outcomes.

Findings

The authors contend that leader feedback inquiry can be appraised as a challenge which then produces beneficial subordinate behaviors (i.e. higher in-role and proactive skill development behaviors). However, leader feedback inquiry can also be appraised as a threat which then elicits detrimental subordinate behaviors (i.e. lower in-role and proactive skill development behaviors). The authors then argue that subordinates with a high promotion focus appraise leader feedback inquiry as challenging, thereby enabling beneficial behaviors. Subordinates with a high prevention focus, by contrast, appraise leader feedback inquiry as threatening, thereby prompting detrimental behaviors.

Originality/value

The authors shed light on the benefits and costs of leader feedback seeking for subordinates. The resulting framework underlines the importance of including individual characteristics and cognitive appraisal processes in research investigating the effects of leader feedback inquiry on subordinate outcomes.
How does regulatory framework impact sectoral performance? A systematic literature review
Authors: Mishra Brajesh , Kumar Avanish
Published: December 2021

Purpose

The regulatory framework may be construed as the existence of supporting infrastructure that assists in control, direction/implementation of a proposed course of law, rule or action. The regulatory order is now more formalized, expert-driven, transparent, independent and pervasive across countries and sectors. As a result, regulatory reforms enable markets to function efficiently by providing a supportive environment for increased investment, private sector growth and market-led economic growth. This study aims to review previous literature for understanding the impact of sectoral regulatory framework on sectoral performance.

Design/methodology/approach

This paper has adopted a systematic literature review to understand dynamics between the sectoral regulatory framework and sectoral performance. While seven multidisciplinary databases were used to identify 51 research articles, the bibliometric research profiling was executed to broaden academic research.

Findings

The results are organized into three broad categories: research context, research area and research methods. The identified articles exhibited association with 12 distinct sectors/industries, with maximum articles belonging to telecom, energy and finance industries. The study has focused on evolution of regulatory studies, impact of regulatory framework on sectoral performance and commonality in regulatory studies. Among the 15 distinct research contexts identified in this systematic literature review (SLR), the highest mapping was registered (from 23 articles) by the research context “impact of regulatory framework on the sector–institutions, infrastructure and performance indicators.”

Practical implications

Public administration researchers are increasingly using mixed methods research approaches to add diverse and novel perspectives on wicked problems. The qualitative approach (grounded theory, action research, phenomenology and participant observations) is appropriate for understanding the native viewpoints of regulatory practitioners and reducing the gap between rigor and relevance.

Originality/value

The study addresses lack of systematic review of articles covering the impact of regulatory framework on sectoral performance encompassing all sectors by, inter alia, collating important bibliometric profiles of the identified articles.
Principals' perspectives of inclusive education involving students with autism spectrum conditions – a Swedish case study
Authors: Lüddeckens Johanna , Anderson Lotta , Östlund Daniel
Published: December 2021

Purpose

The aim of this case study is to describe what commitment and actions are needed in the Swedish school so that principals — within the Swedish school policy framework and with the goal of creating an inclusive school culture and practice — can positively affect schooling for students with disabilities, with a particular focus on students with autism spectrum conditions (ASC). Three research questions guide the study: (1) What commitment and actions do principals consider important for developing an inclusive school for all students, with a particular focus on students with ASC? (2) How do the principals reflect on their own leadership in the development of inclusive education, with a particular focus on students with ASC? (3) Based on the results, what are the implications of the study in practice?

Design/methodology/approach

As part of a three-step data collection method, a snowball sampling was conducted in which n = 6 principals were initially interviewed and the data analyzed by an inductive thematic content analysis.

Findings

(1) Certain structures are needed when planning how to develop mutual values when organizing an inclusive school involving students with ASC, (2) the principals could, at times, feel a sense of loneliness in relation to their superiors and decision-makers and (3) more accountability from educators and greater consideration for the student perspective in decision-making are needed.

Practical implications

It was found that (1) certain structures are needed when planning how to develop mutual values when organizing an inclusive school involving students with ASC, (2) the principals could, at times, feel a sense of isolation in relation to their superiors and decision-makers and (3) more accountability from educators and greater consideration for the student perspective in decision-making are needed.

Originality/value

Index for inclusion and elements from the inclusive leadership model were used in the data collection and analysis.
Impact of COVID-19 on food outlets: symmetric or asymmetric? A case study of Amritsar
Authors: Dhillon Dilpreet Kaur , Kaur Kuldip
Published: December 2021

Purpose

The present study is an intra-industry analysis, which aims to investigate whether the impact of COVID-19 on employment level, clientele rate, liquidity constraints and sustainability aspect of different food outlets is symmetric or asymmetric in nature.

Design/methodology/approach

With the help of well-structured questionnaire, the study has surveyed 80 food outlets in total by interviewing the managers and owners of these outlets. Food outlets have been classified into four categories namely international, national, local and street food outlets. Econometric techniques like MANOVA and Garret ranking have been employed to fulfil the objective of the study.

Findings

The results depict that the impact of COVID-19 on employment level and liquidity constraints is significantly asymmetric amongst different groups of food outlets, even though the decline in extent of clientele is somewhat same for all groups. The survival aspect of outlets also witnesses clear-cut asymmetry in results as big outlets have greater potential to survive for longer if lockdown happens again when compared to street food outlets as their financial availability and stability differ.

Research limitations/implications

The sample size of study is restricted, mainly due to lesser number of national franchise's food outlets available in Amritsar, though other categories of eateries were sufficient in number. Further, the study is restricted only to one district of Punjab state, whereas for future research, inter-district comparison can be done.

Practical implications

The findings reveal that the street food outlets may gain by fostering its online functioning. Similarly national food outlets are encouraged to alter their business strategies to revive their sales against their competitors.

Originality/value

This study is one of the explorer studies to analyse the impact of COVID-19 by making an intra-industry comparison for the eatery industry – considering four different categories of eateries. The classification of eateries helps in analysing whether the employment level, clientele rate, liquidity constraints and survival perspective have been affected symmetrically for the whole eateries industry or does severity of being affected differ asymmetrically. The study makes a contribution by adding a new string of dimension to the existing load of literature in the domain of hospitality.
Preparation and selectivity evaluation of grafted temperature-responsive imprinted composite polyvinylidene fluoride resin membranes for selective adsorption of ReO4−
Authors: Zhang Jun , Ou Xiaojian , Li Lin , Chen Qianqian , Zhang Zifan , Huo Ting , Lin Xiaoyu , Niu Fangfang , Zhao Shengyuan , We Fang , Li Hui , Liu Chunli , Chen Zhenbin , Lu Sujun , Zhang Peng , Zhu Jinian
Published: December 2021

Purpose

A novel grafted temperature-responsive ReO4− Imprinted composite membranes (Re-ICMs) was successfully prepared by using polyvinylidene fluoride (PVDF) resin membranes as substrates, this study aimed to separate and purify ReO effectively.

Design/methodology/approach

Re-ICMs were synthesized by PVDF resin membranes as the substrate, acrylic acid (AA), acrylamide (AM), ethylene glycol dimethacrylate (EGDMA) were functional monomers. The morphology and structure of Re-ICMs were characterized by scanning electron microscope and Fourier transform infrared spectroscopy.

Findings

The maximum adsorption capacity toward ReO4− was 0.1,163 mmol/g and the separation decree had relation to MnO4− was 19.3. The optimal operation conditions were studied detailedly and the results as follows: the molar ratios of AA, AM, EGDMA, ascorbic acid, NH4ReO4, were 0.8, 0.96, 0.02, 0.003 and 0.006. The optimal time and temperature were 20 h and 40°C, respectively. The Langmuir and pseudo-second-order models were fit these adsorption characteristics well.

Practical implications

Rhenium (Re) is mainly used to chemical petroleum and make superalloys for jet engine parts. This study was representing a technology in separate and purify of Re, which provided a method for the development of the petroleum and aviation industry.

Originality/value

This contribution provided a novel method to separate ReO4− from MnO4−. The maximum adsorption capacity was 0.1163 mmol/g at 35°C and the adsorption equilibrium time was within 2 h. Meanwhile, the adsorption selectivity rate ReO4−/MnO4− was 19.3 and the desorption rate was 78.3%. Controlling the adsorption experiment at 35°C and desorption experiment at 25°C in aqueous solution, it could remain 61.3% of the initial adsorption capacity with the adsorption selectivity rate of 13.3 by 10 adsorption/desorption cycles, a slight decrease, varied from 78.3% to 65.3%, in desorption rate was observed.
Erosion characteristics on surface texture of additively manufactured AlSi10Mg alloy in SiO2 quartz added slurry environment
Authors: Demirsöz Recep , Korkmaz Mehmet Erdi , Gupta Munish Kumar , Collado Alberto Garcia , Krolczyk Grzegorz M.
Published: December 2021

Purpose

The main purpose of this work is to explore the erosion wear characteristics of additively manufactured aluminium alloy. Additive manufacturing (AM), also known as three-dimensional (3D) manufacturing, is the process of manufacturing a part designed in a computer environment using different types of materials such as plastic, ceramic, metal or composite. Similar to other materials, aluminum alloys are also exposed to various wear types during operation. Production efficiency needs to be aware of its reactions to wearing mechanisms.

Design/methodology/approach

In this study, quartz sands (SiO2) assisted with oxide ceramics were used in the slurry erosion test setup and its abrasiveness on the AlSi10Mg aluminum alloy material produced by the 3D printer as selective laser melting (SLM) technology was investigated. Quartz was sieved with an average particle size of 302.5 µm, and a slurry environment containing 5, 10 and 15% quartz by weight was prepared. The experiments were carried out at the velocity of 1.88 (250 rpm), 3.76 (500 rpm) and 5.64 m/s (750 rpm) and the impact angles 15, 45 and 75°.

Findings

With these experimental studies, it has been determined that the abrasiveness of quartz sand prepared in certain particle sizes is directly related to the particle concentration and particle speed, and that the wear increases with the increase of the concentration and rotational speed. Also, the variation of weight loss and surface roughness of the alloy was investigated after different wear conditions. Surface roughness values at 750 rpm speed, 10% concentration and 75° impingement angle are 0.32 and 0.38 µm for 0 and 90° samples, respectively, with a difference of approximately 18%. Moreover, concerning a sample produced at 0°, the weight loss at 250 rpm at 10% concentration and 45° particle impact angle is 32.8 mg, while the weight loss at 500 rpm 44.4 mg, and weight loss at 750 rpm is 104 mg. Besides, the morphological structures of eroded surfaces were examined using the scanning electron microscope to understand the wear mechanisms.

Originality/value

The researchers verified that this specific coating condition increases the slurry wear resistance of the mentioned steel. There are many studies about slurry wear tests; however, there is no study in the literature about the quartz sand (SiO2) assisted slurry-erosive wear of AlSi10Mg alloy produced with AM by using SLM technology. This study is needed to fill this gap in the literature and to examine the erosive wear capability of this current material in different environments. The novelty of the study is the use of SiO2 quartz sands assisted by oxide ceramics in different concentrations for the slurry erosion test setup and the investigations on erosive wear resistance of AlSi10Mg alloy manufactured by AM.
Organizational resilience in hospitality family businesses during the COVID-19 pandemic: a qualitative approach
Authors: Schwaiger Katrin , Zehrer Anita , Braun Boris
Published: December 2021

Purpose

This study targeted hospitality family business owners as essential pillars of the tourism industry. How they perceive aspects of the crisis and what they derive organizational resilience from, including the role of their human resources, are explored. Internal and external factors of resilience are analyzed alongside different levels of resilience action.

Design/methodology/approach

The World Health Organization announced coronavirus disease 2019 (COVID-19) as a pandemic on March 11, 2020. In Tirol, the hospitality industry has particularly been affected as tourism businesses find themselves in the challenging times of returning to normal business, corresponding to the fourth phase in Faulkner’s (2001) Tourism Disaster Management Framework. The authors used a qualitative exploratory approach, using semi-structured interviews.

Findings

In taking on a holistic explorative approach, the authors determined several methods used by entrepreneurs in dealing with a pandemic crisis to increase business resilience at a specific stage. Internal and external resilience factors have been detected among three levels of resilience action (personal, regional and governmental). The most surprising result of the semi-structured in-depth interviews was the entrepreneurs’ rather positive outlook.

Originality/value

Generally, this study creates an in-depth understanding of the tourism businesses in their dealing with a global crisis, using family business owners as an exemplary stakeholder group. The authors bridge a gap in the literature by applying a holistic explorative approach in the early stage of a never seen worldwide crisis and by addressing organizational resilience. Three levels of resilience action give new insight into how the beginning of a pandemic crisis is handled and perceived by hospitality family business entrepreneurs.
CloudJS: novel cloud-based design framework for text-file encryption
Authors: Gupta Rhea , Dharadhar Sara , Churi Prathamesh
Published: December 2021

Purpose

Cloud computing is becoming increasingly popular as it facilitates convenient, ubiquitous, on-demand network access to a shared pool of configurable computing resources and applications that can be quickly retrieved and released. Despite its numerous merits, it faces setbacks in data security and privacy. Data encryption is one of the most popular solutions for data security in the cloud. Various encryption algorithms have been implemented to address security concerns. These algorithms have been reviewed along with the Jumbling Salting algorithm and its applications. The framework for using Jumbling Salting to encrypt text files in the cloud environment (CloudJS) has been thoroughly studied and improvised. The purpose of this paper is to implement the CloudJS algorithm, to discuss its performance and compare the obtained results with existing cloud encryption schemes.

Design/methodology/approach

The paper uses six research questions to analyze the performance of CloudJS algorithm in the cloud environment. The research questions are about measuring encryption time and throughput, decryption time and throughput, the ratio of cipher to the plain text of CloudJS algorithm with respect to other Cloud algorithms like AES and DES. For this purpose, the algorithm has been implemented using dockers-containers in the Linux environment.

Findings

It was found that CloudJS performs well in terms of encryption time, decryption time and throughput. It is marginally better than AES and undoubtedly better than DES in these parameters. The performance of the algorithm is not affected by a number of CPU cores, RAM size and Line size of text files. It performs decently well in all scenarios and all resultant values fall in the desired range.

Research limitations/implications

CloudJS can be tested with cloud simulation platforms (CloudSim) and cloud service providers (AWS, Google Cloud). It can also be tested with other file types. In the future, CloudJS algorithm can also be implemented in images and other files.

Originality/value

To the best of the knowledge, this is the first attempt to implement and analysis of a custom encryption algorithm (CloudJS) in the cloud environment using dockers-containers.
Differential DNA methylation across environments has no effect on gene expression in the eastern oyster
Authors: Johnson Kevin M. , Sirovy Kyle A. , Kelly Morgan W.
Published: December 2021

It has been hypothesized that environmentally induced changes to gene body methylation could facilitate adaptive transgenerational responses to changing environments.

We compared patterns of global gene expression (Tag‐seq) and gene body methylation (reduced representation bisulfite sequencing) in 80 eastern oysters Crassostrea virginica from six full‐sib families, common gardened for 14 months at two sites in the northern Gulf of Mexico that differed in mean salinity.

At the time of sampling, oysters from the two sites differed in mass by 60% and in parasite loads by nearly two orders of magnitude. They also differentially expressed 35% of measured transcripts. However, we observed differential methylation at only 1.4% of potentially methylated loci in comparisons between individuals from these different environments, and little correspondence between differential methylation and differential gene expression.

Instead, methylation patterns were largely driven by genetic differences among families, with a PERMANOVA analysis indicating nearly a two orders of magnitude greater number of genes differentially methylated between families than between environments.

An analysis of CpG observed/expected values (CpG O/E) across the C. virginica genome showed a distinct bimodal distribution, with genes from the first cluster showing the lower CpG O/E values, greater methylation and higher and more stable gene expression, while genes from the second cluster showed lower methylation, and lower and more variable gene expression.

Taken together, the differential methylation results suggest that only a small portion of the C. virginica genome is affected by environmentally induced changes in methylation. At this point, there is little evidence to suggest that environmentally induced methylation states would play a leading role in regulating gene expression responses to new environments.
Changes in forest structure drive temperature preferences of boreal understorey plant communities
Authors: Christiansen Ditte Marie , Iversen Lars Lønsmann , Ehrlén Johan , Hylander Kristoffer
Published: December 2021

The local climate in forest understories can deviate substantially from ambient conditions. Moreover, forest microclimates are often characterized by cyclic changes driven by management activities such as clear‐cutting and subsequent planting. To understand how and why understorey plant communities change, both ambient climate change and temporal variation in forest structure have to be considered.

We used inventories from 11,436 productive forest sites in Sweden repeated every 10th year 1993–2017 to examine how variation in forest structure influences changes in the average value of minimum and maximum temperature preferences of all species in a community, that is, community temperature indices (CTIs). We then evaluated to what extent these changes were driven by local extinctions and colonizations, respectively, and to what extent the difference in CTI value between two inventories was related to changes in forest density and in macroclimate. Lastly, we tested whether effects on CTI change by these two drivers were modified by topography, soil moisture and tree species composition.

CTI values of the understorey plant communities increased after clear‐cutting, and decreased during periods when the forest grew denser. During the period immediately after clear‐cutting, changes were predominately driven by colonizations of species with a preference for higher temperatures. During the forest regeneration phase, both colonizations by species preferring lower temperatures and local extinctions of species preferring higher temperatures increased. The change in understorey CTI over 10‐year periods was explained more by changes in forest density, than by changes in macroclimate. Soil moisture, topography and forest tree species composition modified to some extent the effects of changes in forest density and in macroclimate on understorey CTI values.

Synthesis. Via stand manipulation, forest management impacts the effects of regional climate on understorey plant communities. This implies that forest management by creating denser stands locally even can counterbalance the effects of regional changes in climate. Consequently, interpretations of changes in the mean temperature preference of species in forest understorey communities should take forest management regimes into account.
The RIX Wiki: the use of multimedia advocacy to support person‐centred planning in a special needs secondary school – implications for current policy and practice
Authors: Kwiatkowska Gosia , Minnion Andy , Finch Jo
Published: December 2021

The article documents findings from a pilot study undertaken in 2012–2013 in a special needs secondary school in the England, that trialled the use of a multimedia advocacy tool, “The RIX Wiki”. The trial was part of the pathfinder programme, which aimed to reform existing Special Education Needs provision, from the system of statementing to one of developing education health care plans. The reforms became enshrined in the Children and Families Act (2014). The findings were overall positive and helped with person‐centred planning processes. The discussion is framed within current and future policy directives. We argue that multimedia advocacy approaches and software tools, like the “RIX Wiki” will have a continued role to play in ensuring the needs, aspirations and choices of children and young people with learning disabilities remain central.
The impact of gastrostomy feeding tubes on the early childhood education and care of young children
Authors: Swan Charlie
Published: December 2021

This article explores how gastrostomy feeding tubes can impact the early childhood education and care (ECEC) experiences of children. It presents findings from a small‐scale study, which utilised the perspectives of early years educators and parents. The findings indicate that feeding tubes have both an opportunistic and restrictive impact within ECEC. Opportunity was evident through improved nutrition and health, which allowed familial respite and an increased attendance of ECEC. This enabled opportunities for social and explorative interactions. However, the complexities of having and supporting a feeding tube made accessing types of play and exploration challenging. Collaboration between the educators and parents was a crucial tool for success. Educators felt with experience and teamwork they could ensure the needs of children were met innovatively and safely. However, it was concluded clearer advice for attending ECEC settings with a feeding tube would benefit all involved and instil a sense of readiness.
Process optimisation for the mechanised dry crystallisation of rice flake–milk pudding mix
Authors: Panditrao Deshmukh Gajanan , Ravindra Menon Rekha , Jose Naveen , Nath B Surendra , Sivaram Muniandy
Published: December 2021

A novel dry crystallisation approach was attempted to formulate a convenience mix for a rice pasta–milk pudding. A mechanical stirring unit was custom‐designed for the process. The interactive effect of process parameters (steam pressure, rotor speed, process time) on product characteristics was evaluated in terms of colour, particle size distribution, texture and sensory attributes of the mix and the reconstituted product. Optimised process conditions identified using response surface methodology (RSM) were steam pressure of 1.6 kg/cm2 at a scrapper speed of 50 rpm for 5 min. The prepared mix had a moisture content of <5% and was highly acceptable on reconstitution.
Patterns of cervical cancer screening among Medicaid beneficiaries
Authors: Dillon Jacquelyn , Chen Ling , Melamed Alexander , St. Clair Caryn M. , Hou June Y. , Khoury‐Collado Fady , Gockley Allison , Accordino Melissa , Hershman Dawn L. , Wright Jason D.
Published: December 2021

Objective

Cervical cancer screening guidelines have evolved over time with the incorporation of human papillomavirus (HPV) testing along with cytology. Current screening guidelines recommend cytological screening every 3 years or HPV testing with or without cytology every 5 years in women age 30–65 years. We examined the use of cervical cancer screening among average‐risk Medicaid beneficiaries.

Design

Retrospective cohort study.

Population

Women age 30–64 years at average risk for cervical cancer who underwent cervical cancer screening with cytology, co‐testing or primary HPV testing from 2013 to 2016.

Methods

The IBM Watson Health Multi‐State Medicaid MarketScan Database was used. Subsequent screening rates within 3 years of the index test were examined.

Main outcome measure

The rate of repeat cervical cancer screening was analysed using a cumulative incidence function.

Results

A total of 265 083 patients were identified. Overall, 43.1% (n = 114 312) had index co‐testing, 55.2% (n = 146 309) had cytology and 1.7% (n = 4462) had primary HPV testing. The cumulative incidence of early, repeat cervical cancer screening was 3.9% at 12 months, 22.7% at 24 months and 33.3% at 36 months. During the period from 12 to 24 months after follow up, 20.9% of women underwent repeat screening while 19.4% underwent repeat screening 24–36 months after the index test. Among women who did not undergo repeat cervical cancer screening, a yearly gynaecological examination was performed in only 16 627 (10.7%) during year 2 and in 11 116 (8.8%) during year 3.

Conclusion

Among average‐risk Medicaid beneficiaries, cervical cancer screening is frequently overused. Women who do not undergo cervical cancer screening are unlikely to undergo routine gynaecological examination.

Tweetable Abstract

Among average‐risk Medicaid beneficiaries, cervical cancer screening is frequently overused.
Promoting expatriates’ acculturation: the three‐way interactive effect between cross‐cultural training, repatriation practice and leadership consideration
Authors: Xiang Shuting , Wu Shan , Wang Jue , Zhou Qiwei , Ning Nan
Published: December 2021

Acculturation is important in predicting expatriation success, especially for Chinese expatriates. Given the inconsistent findings regarding the effectiveness of cross‐cultural training (CCT) on expatriate acculturation, this study adopts the ‘internal fit’ perspective, which indicates that managerial practices jointly influence employees. Specifically, we investigate how CCT interacts with other internally aligned managerial components, including repatriation practice and leadership consideration, to affect expatriate acculturation. Two survey studies were conducted to test our hypotheses. We gathered cross‐sectional data from 178 Chinese expatriates from three multinational enterprises in study 1 and time‐lagged data from 230 Chinese expatriates from more than 200 multinational enterprises in study 2. Results demonstrate a positive relationship between CCT and expatriates’ acculturation, with repatriation practice strengthening that relationship. Moreover, CCT, repatriation practice and leadership consideration promote expatriates’ acculturation in a synergistic manner. This study contributes to the literature on Chinese expatriates and provides practical implications.
Janus kinase inhibition in induction treatment of anti‐MDA5 juvenile dermatomyositis‐associated rapidly progressive interstitial lung disease
Authors: Chan Ng Poh Lin Pauline , Mopur Archana , Goh Daniel Yam Thiam , Ramamurthy Mahesh Babu , Lim Michael Teik Chung , Lim Lee Kean , Ooi Pei Ling , Ang Elizabeth Youning
Published: December 2021

Tofacitinib has an important role in pediatric rapidly progressive interstitial lung disease (ILD) associated with juvenile dermatomyositis (JDM), an otherwise potentially fatal condition. It may be useful in induction of remission and can be used safely to maintain remission. Serum ferritin and interleukin‐18 are useful markers for tracking activity and response of JDM‐associated ILD.