bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Leaked Crude Oil in a Mountainous Area
Authors: Wang Ke , Peng Jing , Zhao Jue , Hu Bing
Published: September 2022

China–Myanmar oil and gas pipelines in Southwest China guarantee the energy security of China. Due to poor geographical circumstances, the safety of pipelines is seriously threatened by natural disasters. Therefore, there is a crucial, practical significance to establishing a model of leakage and diffusion of crude oil in the mountainous terrain and to conduct related applied studies. In the present study, computational fluid dynamic simulations of the dynamic diffusion process of leaking contaminants on the mountain surface was performed; the influence of the pipe pressure, landform, surface environment and leakage location on diffusion speed and range were discussed carefully. The results indicate that the variation of topographic altitude determines the path of leaking contaminants. Accordingly, an improved algorithm based on the SFD8 algorithm to predict the path of leaking contaminants at a low leakage rate was proposed; this would be instructive for an emergency response to ensure the safety of pipelines.
Multi-Objective Optimization via GA Based on Micro Laser Line Scanning Data for Micro-Scale Surface Modeling
Authors: Rodríguez J. Apolinar Muñoz
Published: September 2022

Industry 4.0 represents high-level methodologies to make intelligent, autonomous, and self-adaptable manufacturing systems. Additionally, the surface modeling technology has become a great tool in industry 4.0 for representing the surface point cloud. Thus, the micro-scale machining technology requires efficient models to represent micro-scale flat and free-form surfaces. Therefore, it is fundamental to perform surface modeling through artificial intelligence for representing small surfaces. This study addressed multi-objective optimization via genetic algorithms and micro laser line projection to accomplish surface models for representing micro-scale flat and free-form surfaces, where an optical microscope system retrieves micro-scale topography via micro laser line coordinates and the multi-objective optimization constructs the flat and free-form surface models through genetic algorithms and micro-scale topography. The multi-objective optimization determines the surface model parameters through exploration and exploitation, and the solution space is deduced via surface data. The surface model generated through the multi-objective optimization fit accurately to the micro-scale target surface. Thus, the proposed technique enhanced the fitting of micro-scale flat and free-form surface models, which were deduced via gray-level images of an optical microscope. This enhancement was validated by a discussion between the multi-objective optimization via genetic algorithms and the micro-scale surface modeling via optical microscope imaging systems.
A Study on the Train Brake Position-Based Control Method for Regenerative Inverters
Authors: Yun Chi-Myeong , Cho Gyu-Jung , Kim Hyungchul , Jung Hosung
Published: September 2022

The use an inverter is one of the representative ways to utilize regenerative braking energy in railway systems. Due to the nature of urban railways that generate a large amount of regenerative energy, the economic advantages are clear. However, in the case of the existing inverter operation method, a method of operating the inverter using the threshold voltage is used, which has a disadvantage in that power cannot be utilized between the no-load voltage and the threshold voltage. Therefore, in this paper, we propose an optimal location selection method and capacity calculation method for installing a regenerative inverter in an urban rail system, and a control method according to the train brake position to increase the regenerative energy utilization rate. First, the inverter capacity and location were selected by selecting the maximum regenerative energy generation for each substation section through the train performance simulation (TPS) based DC power simulation (DCPS). An inverter control method based on train brake position (BP method) is introduced. Finally, PSCAD/EMTDC, a power analysis program, was used to verify the proposed method. As a result, the use of regenerative energy by an inverter increased by about 62.6%, and more energy was saved at nearby substations through the BP method.
Overview of Biomass-to-Energy Supply and Promotion Policy in Taiwan
Authors: Lee Yu-Ru , Tsai Wen-Tien
Published: September 2022

During the past two decades, Taiwan’s average dependence on imported energy was 97.6%, thus pushing the government to promote the indigenous energy supply. In this regard, the energy policy and regulatory incentives for promoting biomass-to-energy or bioenergy have been recently established. In this work, the updated statistics of biomass-derived waste and energy supply from biomass during the period of 2005–2021 were analyzed using national/official reports. It was found that the annual agricultural waste amounts in Taiwan ranged from 4.5 to 5.2 million metric tons, and about 80% of those were generated from rice-derived residues (rice straw and rice husk) and livestock/poultry-derived waste (manure). In addition, a decreasing trend was observed in the indigenous bioenergy supply, mostly from the solid-type biomass resources, including waste wood, rice husk, and sugarcane bagasse. In order to expand bioenergy diversification, the central competent authorities, including the Ministry of Economic Affairs (MOEA), Council of Agriculture (COA), and Environmental Protection Administration (EPA), have announced the relevant policies for bioenergy promotion under the authorization of acts. Among them, the Renewable Energy Development Act is the legal foundation for promoting bioenergy and its industry development through economic incentives like feed-in-tariff (FIT), installation supports (or subsidies), and electrical grid connection.
Digital Twins for Real-Time Scenario Analysis during Well Construction Operations
Authors: Saini Gurtej Singh , Fallah AmirHossein , Ashok Pradeepkumar , van Oort Eric
Published: September 2022

Well construction is a complex multi-step process that requires decision-making at every step. These decisions, currently made by humans, are inadvertently influenced by past experiences and human factor issues, such as the situational awareness of the decision-maker. This human bias often results in operational inefficiencies or safety and environmental issues. While there are approaches and tools to monitor well construction operations, there are none that evaluate potential action sequences and scenarios and select the best possible sequence of actions. This paper defines a generalized iterative methodology for setting up a digital twin to address this shortcoming. Depending on its application, the objectives and constraints around the twin are formulated. The digital twin is then built using a cyclical process of defining the required outputs, identifying and integrating the necessary process models, and aggregating the required data streams. The twin is set up such that it is predictive in nature, thus enabling scenario analysis. The method is demonstrated here by setting up twinning systems for two different categories of problems. First, an integrated multi-model twin to replicate borehole cleaning operations for stuck-pipe prevention is developed and tested. Second, the creation, implementation, and testing of a twinning system for assisting with operational planning and logistics is demonstrated by considering the time it takes to drill a well to total depth (TD). These twins are also used to simulate multiple future scenarios to quantify the effects of different actions on eventual outcomes. Such systems can help improve operational performance by allowing more informed human, as well as automated, decision-making. Development of a system for well construction operations that integrates multiple sources of information with process and equipment models to quantify the system state and analyzes different scenarios by evaluating action sequences is a novel contribution of this paper. The approach presented here can be applied to the construction of digital twins for any well construction operation.
Assessment of a Hybrid Eulerian–Lagrangian CFD Solver for Wind Turbine Applications and Comparison with the New MEXICO Experiment
Authors: Spyropoulos Nikos , Papadakis George , Prospathopoulos John M. , Riziotis Vasilis A.
Published: September 2022

In this paper, the hybrid Lagrangian–Eulerian solver HoPFlow is presented and evaluated against wind tunnel measurements from the New MEXICO experiment. In the paper, the distinct solvers that assemble the HoPFlow solver are presented, alongside with details on their mutual coupling and interaction. The Eulerian solver, MaPFlow, solves the compressible Navier–Stokes equations under a cell-centered finite-volume discretization scheme, while the Lagrangian solver uses numerical particles that carry mass, pressure, dilatation and vorticity as flow markers in order to represent the flow-field by following their trajectories. The velocity field is calculated with the use of the decomposition theorem introduced by Helmholtz. Computational performance is enhanced by utilizing the particle mesh (PM) methodology in order to solve the Poisson equations for the scalar potential ϕ and the stream function ψ→. The hybrid solver is tested in 3-D unsteady simulations concerning the axial flow around the wind turbine (WT) model rotor tested in the New MEXICO experimental campaign. Simulation results are presented as integrated rotor loads, radial distribution of aerodynamic forces and moments and pressure distributions at various span-wise positions along the rotor blades. Comparison is made against experimental data and computational results produced by the pure Eulerian solver. A total of 5 PM nodes per chord length of the blade section at 75% have been found to be sufficient to predict the loading at the tip region of the blade with great accuracy. Discrepancies with respect to measurements, observed at the root and middle sections of the blade, are attributed to the omission of the spinner geometry in the simulations.
Effect of Storage Temperature and Time on Biogenic Amines in Canned Seafood
Authors: Qu Yinghong , Wang Jingyu , Liu Zhidong , Wang Xichang , Zhou Huimin
Published: September 2022

Biogenic amines in canned seafood are associated with food quality and human health. In this study, a total of nine biogenic amines (histamine (HIS), phenylethylamine (PHE), tyramine (TYM), putrescine (PUT), cadaverine (CAD), tryptamine (TRY), spermine (SPM), spermidine (SPD), and octopamine (OCT)) were used as standards. The biogenic amines of five canned seafood species (canned mud carp, canned sardine, canned mantis shrimp, canned scallop, and canned oyster) were investigated every three months for 12 months at different storage temperatures (4, 10, 25, and 30 °C). The biogenic amine contents were determined by the ultrasound-assisted dispersive solid-phase extraction method combined with reversed-phase high-performance liquid chromatography-photodiode array detection (UADSPE-RPLC-PDA). These results showed a detection rate of 100, 60, and 40% for HIS, PHE, PUT, and TYM; CAD, SPM, and SPD; OCT in all the samples, respectively. The contents of histamine and tyramine exceeded the recommended maximum limits (50 and 100 mg kg−1) in the canned mud carp and canned scallop when stored at 30 °C, indicating their potential health risks (p < 0.05). This result also indicates that low temperatures could inhibit the BAs content of canned seafood during storage. Overall, storage temperature and time can be used as the primary means to monitor and control the quality and safety of canned seafood.
Health Benefits and Risks of Consuming Spices on the Example of Black Pepper and Cinnamon
Authors: Newerli-Guz Joanna , Śmiechowska Maria
Published: September 2022

The aim of this study is to present the benefits and risks associated with the consumption of black pepper and cinnamon, which are very popular spices in Poland. The article presents the current state of knowledge about health properties and possible dangers, such as liver damage, associated with their consumption. The experimental part presents the results of the research on the antioxidant properties against the DPPH radical, which was 80.85 ± 3.84–85.42 ± 2.34% for black pepper, and 55.52 ± 7.56–91.87 ± 2.93% for cinnamon. The total content of polyphenols in black pepper was 10.67 ± 1.30–32.13 ± 0.24 mg GAE/g, and in cinnamon 52.34 ± 0.96–94.71 ± 3.34 mg GAE/g. In addition, the content of piperine and pepper oil in black pepper was determined, as well as the content of coumarin in cinnamon. The content of piperine in the black pepper samples was in the range of 3.92 ± 0.35–9.23 ± 0.05%. The tested black pepper samples contained 0.89 ± 0.08–2.19 ± 0.15 mL/100 g d.m. of essential oil. The coumarin content in the cinnamon samples remained in the range of 1027.67 ± 50.36–4012.00 ± 79.57 mg/kg. Taking into account the content of coumarin in the tested cinnamon samples, it should be assumed that the majority of cinnamon available in Polish retail is Cinnamomum cassia (L.) J. Presl.
Effect of Cephalosporin Antibiotics on the Activity of Yoghurt Cultures
Authors: Navrátilova Pavlina , Borkovcova Ivana , Stastkova Zora , Bednarova Ivana , Vorlova Lenka
Published: September 2022

The presence of antibiotics in milk is a significant problem affecting the technological safety of dairy products. The aim of the study was to determine the sensitivity of yoghurt cultures to residual levels of selected cephalosporin antibiotics (cephalexin, cefoperazone, cefquinome, cefazolin, and ceftiofur). Five yoghurt cultures were selected containing strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Artificially fortified milk samples (whole pasteurized milk; 85 °C; 3–5 s) with cephalosporins at a concentration of the maximum residue limit were used to evaluate the sensitivity of the yoghurt cultures by monitoring the pH, titratable acidity, and the concentration of selected organic acids (lactic, pyruvic, citric, acetic, orotic, oxalic, formic, uric, and succinic acids) at the end of fermentation (43 °C; 4–5.5 h; pH ≤ 4.6). The titratable acidity was determined by the Soxhlet–Henkel method and the organic acid concentration was monitored by reversed-phase HPLC. Ceftiofur had the greatest effect on the yoghurt culture activity, with a statistically highly significant effect (p < 0.05) on the pH, titratable acidity, and the content of lactic, pyruvic, and acetic acids in all cultures. Other cephalosporins also showed an inhibitory effect on yoghurt metabolism as seen by the evaluation of the lactic and pyruvic acid concentrations.
Bioactive Compounds, Health Benefits and Food Applications of Grape
Authors: Zhou Dan-Dan , Li Jiahui , Xiong Ruo-Gu , Saimaiti Adila , Huang Si-Yu , Wu Si-Xia , Yang Zhi-Jun , Shang Ao , Zhao Cai-Ning , Gan Ren-You , Li Hua-Bin
Published: September 2022

Grape (Vitis vinifera L.) is one of the most popular fruits worldwide. It contains various bioactive compounds, such as proanthocyanidins, anthocyanins, flavonols, phenolic acids and stilbenes, the contents of which could vary considerably in grape skin, pulp and seed. Many studies have revealed that grape possesses a variety of health benefits, such as antioxidant, anti-inflammatory, gut-microbiota-modulating, anticancer and cardioprotective effects. Grape is eaten as fresh fruit and is also used as raw material to produce various products, such as wine, grape juice and raisins. Moreover, the byproducts of grape, such as grape pomace and grape seed, have many applications in the food industry. In this paper, the bioactive compounds in grape are briefly summarized based on literature published in recent years. In addition, the health benefits of grape and its bioactive components are discussed, with special attention paid to the underlying mechanisms. Furthermore, the applications of grape in the food industry are elucidated, especially the applications of grape pomace and grape seed. This paper can contribute to understanding the health benefits and mechanisms of grape and its bioactive compounds, as well as the promotion of the use of grape in the food industry.
Growth and Spoilage Potential of an Aeromonas salmonicida Strain in Refrigerated Atlantic Cod (Gadus morhua) Stored under Various Modified Atmospheres
Authors: Hoel Sunniva , Lerfall Jørgen , Jakobsen Anita Nordeng
Published: September 2022

Aeromonas spp. are ubiquitous aquatic bacteria, frequently isolated from seafood. The growth and spoilage potential of an inoculated strain of Aeromonas salmonicida (SU2) in Atlantic cod (Gadus morhua) fillets were assessed as a function of various modified atmospheres (MA) (CO2 (67%) with O2 (33%) or N2 (33%), and vacuum packaging (VP)) under refrigerated storage (4 °C) for 13 days. Additionally, the general microbiological quality was assessed by quantifying total aerobic psychrotrophic count (PC), total aerobic plate count (APC), and H2S-producing bacteria. A selection of spoilage metabolites was quantified by nuclear magnetic resonance (NMR) spectroscopy. The MA containing CO2/O2 and CO2/N2 significantly inhibited the growth of the inoculated strain throughout storage (p < 0.001), whereas VP allowed for a 3-log increase of Aeromonas in 13 days. No significant effect of the inoculation with A. salmonicida on spoilage metabolite production was detected. Applying O2 instead of N2 in the CO2-enriched atmosphere resulted in a significantly lower PC for 10 days, and H2S-producing bacteria were inhibited in both MAs compared to vacuum. This study provides data that can be used as a basis to further elucidate the role of bacteria belonging to the genus Aeromonas as potential spoilage organisms in marine fish species.
Resistant Protein: Forms and Functions
Authors: Zannini Emanuele , Sahin Aylin W. , Arendt Elke K.
Published: September 2022

Several global health risks are related to our dietary lifestyle. As a consequence of the overconsumption of ultra-processed and highly digestible protein (150–200% of the recommended value), excess dietary proteins reach the colon, are hydrolysed to peptides and amino acids by bacterial proteases and fermented to various potentially toxic end products. A diet reformulation strategy with reduced protein content in food products appears to be the most effective approach. A potential approach to this challenge is to reduce food digestibility by introducing resistant protein into the diet that could positively influence human health and gut microbiome functionality. Resistant protein is a dietary constituent not hydrolysed by digestive enzymes or absorbed in the human small intestine. The chemical conformation and the amino acid composition strictly influence its structural stability and resistance to in vivo proteolysis and denaturation. Responding to the important gap in our knowledge regarding the digestibility performance of alternative proteins, we hypothesise that resistant proteins can beneficially alter food functionality via their role in improving metabolic properties and health benefits in human nutrition, similar to fibres and resistant starches. A multidisciplinary investigation of resistant protein will generate tremendous scientific impact for other interlinked societal, economic, technological and health and wellbeing aspects of human life.
The Formation, Structural Characteristics, Absorption Pathways and Bioavailability of Calcium–Peptide Chelates
Authors: An Jiulong , Zhang Yinxiao , Ying Zhiwei , Li He , Liu Wanlu , Wang Junru , Liu Xinqi
Published: September 2022

Calcium is one of the most important mineral elements in the human body and is closely related to the maintenance of human health. To prevent calcium deficiency, various calcium supplements have been developed, but their application tends to be limited by low calcium content and highly irritating effects on the stomach, among other side effects. Recently, calcium–peptide chelates, which have excellent stability and are easily absorbed, have received attention as an alternative emerging calcium supplement. Calcium-binding peptides (CaBP) are usually obtained via the hydrolysis of animal or plant proteins, and calcium-binding capacity (CaBC) can be further improved through chromatographic purification techniques. In calcium ions, the phosphate group, carboxylic group and nitrogen atom in the peptide are the main binding sites, and the four modes of combination are the unidentate mode, bidentate mode, bridging mode and α mode. The stability and safety of calcium–peptide chelates are discussed in this paper, the intestinal absorption pathways of calcium elements and peptides are described, and the bioavailability of calcium–peptide chelates, both in vitro and in vivo, is also introduced. This review of the research status of calcium–peptide chelates aims to provide a reasonable theoretical basis for their application as calcium supplementation products.
Characterization of the Key Aroma Volatile Compounds in Nine Different Grape Varieties Wine by Headspace Gas Chromatography–Ion Mobility Spectrometry (HS-GC-IMS), Odor Activity Values (OAV) and Sensory Analysis
Authors: Cao Weiyu , Shu Nan , Wen Jinli , Yang Yiming , Jin Yuning , Lu Wenpeng
Published: September 2022

During this study, the physicochemical properties, color, and volatile aroma compounds of the original wines produced from the grape varieties ‘Hassan’, ‘Zuoshaner’, ‘Beibinghong’, ‘Zuoyouhong’, ‘Beta’, ‘Shuanghong’, ‘Zijingganlu’, ‘Cabernet Sauvignon’, and ‘Syrah’ were determined and sensory evaluation was performed. Results indicated that ‘Hassan’ contained the most solids, ‘Zuoshaner’ produced the most total acid, residual sugar, total anthocyanin, and total phenol, and ‘Shuanghong’ produced the most tannin. Calculation of the chroma and hue of the wines according to the CIEL*a*b* parameters revealed that the ‘Cabernet Sauvignon’ wines were the brightest of the nine varieties and that the ‘Zuoshaner’ wines had the greatest red hue and yellow hue and the greatest saturation’. A total of 52 volatile compounds were identified and quantified in nine wine samples by HS-GC-IMS analysis, with the most significant number of species detected being 20 esters, followed by 16 alcohols, 8 aldehydes, four ketones, one terpene, and one furan, with the highest total volatile compound content being ‘Beta’. A total of 14 volatile components with OAV (odor activity value) >1 were calculated using the odor activity value (OAV) of the threshold of the aromatic compound, and the OPLS-DA analysis was performed by orthogonal partial least squares discriminant analysis (OPLS-DA) using the OAV values of the compounds with OAV values >1 as the Y variable. The VIP (Variable Importance in Projection) values of six compounds, ethyl isobutyrate, ethyl hexanoate-D, 2-methylpropanal, ethyl octanoate, ethyl butanoate-D, and Isoamyl acetate-D, were calculated to be higher than one between groups, indicating that these six compounds may influence aroma differences. It is essential to recognize that the results of this study have implications for understanding the quality differences between different varieties of wines and for developing wines that have the characteristics of those varieties.
The Consumption of Insects in Switzerland: University-Based Perspectives of Entomophagy
Authors: Penedo Aline Oliveira , Bucher Della Torre Sophie , Götze Franziska , Brunner Thomas A. , Brück Wolfram Manuel
Published: September 2022

Although insects have long been part of the human diet in many countries, they are poorly received and accepted in European and North American countries. Therefore, this cross-sectional observational study, based on a structured questionnaire, aimed to evaluate the level of acceptability of entomophagy among young adults in a Swiss university context. The variable “acceptability of consuming insects” (ACI) was calculated according to the perception of entomophagy of each participant. The ACI was related to various socio-demographic and behavioral aspects. A total of 290 responses were validated and analyzed. The mean ACI score was 3.7 out of 6.0 (SD 1.1). Most participants responded that the most likely reason for eating insect foods was curiosity. The most common reason for not eating such foods was disgust. None of the socio-demographic variables showed a significant association with ACI. Generally, participants in this study showed a potential interest in entomophagy—on a theoretical level, as measured here by the ACI. In practice, however, there are still barriers, including disgust, which contribute to the low consumption of these foods, at least in Switzerland.
Properties of MAXI J1348-630 during Its Second Outburst in 2019
Authors: Bhowmick Riya , Debnath Dipak , Chatterjee Kaushik , Jana Arghajit , Nath Sujoy Kumar
Published: September 2022

The newly discovered galactic black hole candidate (BHC) MAXI J1348-630 showed two major outbursts in 2019, just after its discovery. Here, we provide a detailed spectral and temporal analysis of the less-studied second outburst using archive data from multiple satellites, namely Swift, MAXI, NICER, NuSTAR and AstroSat. The outburst continued for around two and a half months. Unlike the first outburst from this source, this second outburst was a ‘failed’ one. The source did not transition to soft or intermediate spectral states. During the entire outburst, the source was in the hard state with high dominance of non-thermal photons. The presence of strong shocks are inferred from spectral fitting using a TCAF model. In NuSTAR spectra, weak reflection is observed from spectral fitting. Low-frequency quasi-periodic oscillations are also detected in AstroSat data.
Rheological Behavior of Passion Fruit (Passiflora edulis) Peel Extract
Authors: Arrieta-Durango Carlos , Henao-Rivas Luis , Andrade-Pizarro Ricardo
Published: September 2022

In this work, the rheological behavior of passion fruit peel extract was determined at different temperatures (5–40 °C) and peel content in the extract (40–55% w/w). The extract was obtained after blanching the passion fruit peels at 95 °C for 5 min, then they were crushed to reduce their size, water was added, and finally, they were subjected to liquefaction and subsequent filtration. Rheological measurements were made using a rheometer with a plate and plate geometry. Extract samples were adequately described by the power-law model exhibiting pseudoplastic behavior, without the presence of thixotropy. The temperature did not influence the flow behavior index, but the consistency coefficient did. The dynamic study (the temperature sweep test) showed that passion fruit peel extract exhibits a more elastic than viscous behavior, typical of a gel.
Hydrogel and Effects of Crosslinking Agent on Cellulose-Based Hydrogels: A Review
Authors: Nasution Halimatuddahliana , Harahap Hamidah , Dalimunthe Nisaul F. , Ginting M. Hendra S. , Jaafar Mariatti , Tan Orlando O. H. , Aruan Hotmauli K. , Herfananda Alief L.
Published: September 2022

Hydrogels are hydrophilic polymer materials that can swell but are insoluble in water. Hydrogels can be synthesized with synthetic or natural polymers, but natural polymers are preferred because they are similar to natural tissues, which can absorb a high water content, are biocompatible, and are biodegradable. The three-dimensional structure of the hydrogel affects its water insolubility and ability to maintain its shape. Cellulose hydrogels are preferred over other polymers because they are highly biocompatible, easily accessible, and affordable. Carboxymethyl cellulose sodium (CMCNa) is an example of a water-soluble cellulose derivative that can be synthesized using natural materials. A crosslinking agent is used to strengthen the properties of the hydrogel. Chemical crosslinking agent is used more often than physical crosslinking agent. In this review, article, different types of crosslinking agents are discussed based on synthetic and natural crosslinking agents. Hydrogels that utilize synthetic crosslinking agent have advantages, such as adjustable mechanical properties and easy control of the chemical composition. However, hydrogels that use natural crosslinking agent have better biocompatibility and less latent toxic effect.
A Temperature/pH Double-Responsive and Physical Double-Crosslinked Hydrogel Based on PLA and Histidine
Authors: Wu Qingrong , Fu Yu , Yang Wanying , Liu Shouxin
Published: September 2022

Hydrogel is a good drug carrier, widely used in the sustained-release aspect of tumor drugs, which can achieve the continuous release of drugs to the tumor sites. In this study, diethylene glycol monomethyl ether methacrylate (MEO2MA) and poly (ethylene glycol) methyl ether methacrylate (OEGMA) are temperature-sensitive monomers. N-Methacryloyl-L-Histidine (Mist) is pH sensitive monomer and ligand for metal coordination bond. The temperature-sensitive monomers and pH sensitive monomer with stereocomplex of modified polylactic acid (HEMA-PLLA30/PDLA30) were mixed, under 2,2’-azobis (2-methylpropionitrile) (AIBN) as radical initiator, polymer was formed by free-radical polymerization. The polymer was then immersed in ZnSO4 solution, the imidazole group of Mist monomer forms a tridentate metal coordination bond with Zn2+, temperature/pH double-responsive and physical double-crosslinked hydrogel was finally obtained. Comparing the hydrogen bond hydrogel, hydrogen bond and metal coordination bond double crosslinking hydrogel, metal coordination bond hydrogel, testing thermal stability, viscoelasticity, swelling, and morphology of three hydrogels. In addition, using UV-Visible spectroscopy (UV-Vis) to test the sustained release of the hydrophobic drug doxorubicin hydrochloride (DOX-HCl) in the human tumor environment (37 °C, pH = 5). We found that the temperature/pH double-responsive and physical double-crosslinked hydrogel had the most potential for the sustained drug release.
Characterization of Phase Change Materials Fabricated with Cross-Linked Graphene Aerogels
Authors: Yu Chengbin , Song Young Seok
Published: September 2022

3D porous graphene aerogel exhibits a high surface area which can hold plenty of pure phase change material (PCM) into the internal space. In order to maintain the flexibility of PCM without volume shrinkage under the external force, cross-linked graphene aerogel was prepared by the cysteamine vapor method. The cross-linked graphene aerogel had a high stress–strain durability and chemical stability for infiltrating PCM to produce a form-stable PCM composite. The latent heat of PCM is one of the elements to estimate the capacity of PCM thermal energy storage (TES) during the phase transition process. The cross-linked graphene aerogel-supported PCM composite showed a great TES to be utilized in thermal-to-electrical energy harvesting. The cross-linked graphene aerogel also had an excellent mechanical property of preventing damage at a high temperature.
Forensic Applications of Markers Present on the X Chromosome
Authors: Garcia Fernanda M. , Bessa Bárbara G. O. , dos Santos Eldamária V. W. , Pereira Julia D. P. , Alves Lyvia N. R. , Vianna Lucas A. , Casotti Matheus C. , Trabach Raquel S. R. , Stange Victor S. , Meira Débora D. , Louro Iuri D.
Published: September 2022

Microsatellite genetic markers are the gold standard for human genetic identification. Forensic analyses around the world are carried out through protocols using the analysis of STR markers in autosomal chromosomes and in the Y chromosome to solve crimes. However, these analyses do not allow for the resolution of all cases, such as rape situations with suspicion of incest, paternity without a maternal sample for comparison, and biological traces with DNA mixture where the profile sought is female, among other situations. In these complex cases, the study of X-chromosome STR markers significantly increases the probability of identification by complementing the data obtained for autosomal and Y-chromosome markers, due to the unique structure of the X chromosome and its exclusive method of inheritance. However, there are currently no validated Brazilian protocols for this purpose, nor are there any population data necessary for statistical analyses that must be included in the issuance of expert reports. Thus, the aim of this article is to provide a literary review of the applications of X-chromosomal markers in population genetics.
Comparative Transcriptome Analysis of Slow-Twitch and Fast-Twitch Muscles in Dezhou Donkeys
Authors: Li Yan , Ma Qingshan , Shi Xiaoyuan , Yuan Wenmin , Liu Guiqin , Wang Changfa
Published: September 2022

The skeletal muscle fiber profile is closely related to livestock meat quality. However, the molecular mechanisms determining muscle fiber types in donkeys are not completely understood. In this study, we selected the psoas major muscle (PM; mainly composed of oxidative-type muscle fibers) and biceps femoris muscle (BF; mainly composed of glycolytic-type muscle fibers) and systematically compared their mRNA and microRNA transcriptomes via RNA-seq. We identified a total of 2881 differentially expressed genes (DEGs) and 21 known differentially expressed miRNAs (DEmiRs). Furthermore, functional enrichment analysis showed that the DEGs were mainly involved in energy metabolism and actin cytoskeleton regulation. The glycolysis/gluconeogenesis pathway (including up-regulated genes such as PKM, LDHA, PGK1 and ALDOA) was more highly enriched in BF, whereas the oxidative phosphorylation pathway and cardiac muscle contraction (including down-regulated genes such as LDHB, ATP2A2, myosin-7 (MYH7), TNNC1, TPM3 and TNNI1) was more enriched in PM. Additionally, we identified several candidate miRNA–mRNA pairs that might regulate muscle fiber types using the integrated miRNA–mRNA analysis. Combined with the results of protein–protein interaction (PPI) analysis, some interesting DEGs (including ACTN3, TNNT3, TPM2, TNNC2, PKM, TNNC1 and TNNI1) might be potential candidate target genes involved in the miRNA-mediated regulation of the myofibril composition. This study is the first to indicate that DEmiRs, especially eca-miR-193a-5p and eca-miR-370, and potential candidate target genes that are mainly involved in actin binding (e.g., ACTN3, TNNT3 and TNNC1) and the glycolysis/gluconeogenesis pathways (e.g., PKM) might coregulate the myofibril composition in donkeys. This study may provide useful information for improving meat quality traits in Dezhou donkeys.
Relationships between Uncoupling Protein Genes UCP1, UCP2 and UCP3 and Irisin Levels in Residents of the Coldest Region of Siberia
Authors: Nikanorova Alena A. , Barashkov Nikolay A. , Pshennikova Vera G. , Gotovtsev Nyurgun N. , Romanov Georgii P. , Solovyev Aisen V. , Kuzmina Sargylana S. , Sazonov Nikolay N. , Fedorova Sardana A.
Published: September 2022

Currently, it is known that irisin can participate in the processes of thermoregulation and browning of adipose tissue, and, therefore, it is possible that it is involved in the microevolutionary mechanisms of adaptation to a cold. The aim of this study is to investigate the relationship between the uncoupling protein genes (UCP1, UCP2, UCP3) and the irisin levels in the residents of the coldest region of Siberia. The sample consisted of 279 Yakut people (185 females, 94 males, average age 19.8 ± 2.03 years). The females plasma irisin concentration was 8.33 ± 2.74 mcg/mL and the males was 7.76 ± 1.86 mcg/mL. Comparative analysis of irisin levels with the genotypes of six studied SNP-markers in females revealed a significant association of irisin with rs1800849-UCP3. The TT genotype of rs1800849 was associated with elevated levels of irisin (p = 0.01). It was also found that this TT genotype in females was associated with reduced weight and height (p = 0.03). We searched for natural selection signals for the T-allele rs1800849-UCP3; as a result of which, it was found that this allele has a significantly high frequency of distribution in northern (45%, CI: 0.42–0.484) compared with southern Asian populations (28%, CI: 0.244–0.316) (p = 0.01). The results obtained indicate the probable involvement of irisin and the UCP3 gene in thermoregulation, and the spread of its allelic variants is probably related to adaptation to a cold climate.
Unraveling the Drivers Controlling the Transient and Seasonal CO2 Dynamic in a Shallow Temperate Cave
Authors: Fernandez-Cortes Angel , Martin-Pozas Tamara , Cuezva Soledad , Cañaveras Juan Carlos , Saiz-Jimenez Cesareo , Sanchez-Moral Sergio
Published: September 2022

Understanding the dynamics and spatial distribution of gases in the subterranean atmospheres is essential to increase the reliability of carbon balances in karst ecosystems or the paleoclimate reconstructions based on cave deposits. This scientific information is also very valuable for cave managers to ensure the safety of visitors and the conservation of the subterranean heritage. Through a comprehensive monitoring of the main air parameters in a shallow temperate cave, we decipher the physical drivers and mechanisms involved in the CO2 and radon exchange between the cave and the outer atmosphere, and how this process is triggered by the changes of local weather. Our results reveal that the biphasic infiltration (water plus air) in the network of penetrative structures from the overlying soil and host rock exercise remarkable control over the cave environment, delaying the thermal response of the cave air to the outer climate-driven changes and also the gaseous transfer between the cave atmosphere and the exterior. The cave location concerning the karstified outcrop determines that this subterranean site acts as a gas emitter during summer, which is contrary to what happens in many other caves. Prominent gas entrapment at a micro-local level is also registered in some upper galleries.
Peer Academic Supports for Success (PASS) for College Students with Mental Illness: Open Trial
Authors: Davis Maryann , Hutchinson Dori S. , Cherchia Paul , Golden Laura , Morrison Emily , Baczko Amanda
Published: September 2022

Increasing numbers of college students have serious mental health conditions, but their dropout rates are high and debt accrual is common. A well-specified intervention that colleges can directly offer their undergraduates with serious mental health conditions that sustains their academic persistence is greatly needed. The Peer Academic Supports for Success (PASS) coaching model was developed to address this need. This study’s goal was to conduct an open trial of the initial PASS model to test the feasibility of the model and research methods in preparation for more rigorous testing. Ten college juniors and seniors, with and without lived mental health experience, were hired, trained, and supervised to be PASS peer coaches. Twelve undergraduate students with academically impairing mental health conditions served as study participants and received PASS. Student data were collected at baseline and two semesters post baseline. Intervention feasibility data were assessed through coach report. Results indicate PASS can be delivered with fidelity by peer coaches, can attract and retain students, and is safe. Results also suggest that PASS has significant effects on most of the targeted proximal outcomes. The PASS findings are promising as a college-based intervention to support young adult students with mental health conditions.
The Sources of Research Self-Efficacy in Postgraduate Nursing Students: A Qualitative Study
Authors: Gong Jiali , Chen Meizhen , Li Qiuping
Published: September 2022

Postgraduate students are required to undertake a series of research assignments. Research self-efficacy is regarded as the sense of faith in their ability to accomplish research tasks. However, it is unclear how research self-efficacy plays a role in second-year nursing postgraduate students who have been enrolled for a period of time and have not yet met graduation requirements. This study aims to explore the sources of second-year nursing postgraduate students’ research self-efficacy in response to research tasks. A descriptive phenomenological approach was used in this study. A total of 13 second-year nursing postgraduate students participated in semi-structured interviews using a purposive sampling method. Content analysis was applied to analyze the interview data after verbatim transcription. Participants stated that they had gained a great deal of support but were somewhat less confident. Three themes were refined: (a) intrinsic sources of research self-efficacy (differences in cognitive ability, internal driving force, with successful experience), (b) extrinsic sources of research self-efficacy (family support, peer support, mentor support), and (c) unmet support may cause low self-confidence (inadequate self-support, inadequate extrinsic-support, lack of motivation from successful experiences). The postgraduate students in this study accepted the objectives of the master’s training and actively practiced research exploration. They were motivated to persevere through their internal and external support, albeit with some negative aspects that deserve our attention. Interventions for postgraduate students’ cognitive awareness, constant support during their postgraduate career, and finally, guiding summaries may have a positive impact on their self-efficacy.
Editorial Comment on Special Issue—COVID-19 after One Year of Clinical Experience: Unexpected Clinical Presentations or Complications and Post-COVID-19 Clinical Features
Authors: Di Micco Pierpaolo , Annunziata Anna , Fiorentino Giuseppe
Published: September 2022

Beginning in 2020, the COVID-19 pandemic caused by SARS-CoV-2 remains ongoing [...]
Determinants of Protective Healthcare Services Awareness among Female Syrian Refugees in Turkey
Authors: Balcilar Mehmet , Gulcan Canan
Published: September 2022

War-related migration may deprive people of access to a regular healthcare system and cause new diseases to be battled. Since refugee women are more vulnerable to diseases during this period, protective healthcare services awareness is critical for early disease diagnosis. Following the civil war that triggered the migration of millions of Syrians, an extensive survey was undertaken in coordination with the World Health Organization Country Office in Turkey to explore the health status of Syrian refugees in Turkey. Employing the survey data, we aimed to investigate the determinants of the awareness of protective health services (Pap smear test, mammogram, HIV test) among female Syrian refugees. Logit regression analysis was applied in order to investigate the determinants of the awareness of protective health services among the female refugee population. The results revealed a notably low rate of awareness of protective health services among female Syrian refugees. Furthermore, the association of explanatory variables, including socioeconomic factors, healthcare use, and health literacy with the protective health services awareness, was found to be significant.
The Global Impact of COVID-19 on Threat Appraisals
Authors: Mattiuzzi Camilla , Lippi Giuseppe
Published: September 2022

We planned an infodemiological analysis to estimate the global impact of coronavirus disease 2019 (COVID-19) on threat appraisals. We accessed Google Trends using the search terms “Anxiety”, “Distress”, “Fear”, “Rumination”, “Stress” and “Worry” within the “topic” domain, setting the geographical location to “worldwide”, between July 2017 and July 2022. The weekly Google Trends score for the six search terms, thus, mirroring Web popularity and probable prevalence, was compared between the two search periods, “pre-COVID” (between July 2017 and February 2020) and COVID (between March 2020 and July 2022), thus, reflecting the volume of searches before and during the ongoing COVID-19 pandemic. The median weekly Google Trends score of all these search terms significantly increased during the COVID-19 pandemic, i.e., anxiety by 22%, distress by 13%, fear by 9%, rumination by 18%, stress by 13% and worry by 20%. With variable strength, the weekly Google Trends scores of each search term were found to be significantly associated (all p < 0.001). We can, hence, conclude that the enhanced burden of threat appraisals observed after SARS-CoV-2 spread leads the way to establish preventive, diagnostic and therapeutic measures in order to limit the unfavorable mental health consequences caused by the ongoing COVID-19 pandemic.
Long Live the Step Pyramid!
Authors: Kuraszkiewicz Kamil Omar
Published: September 2022

The paper discusses the role of the funerary complex built for Netjerykhet (Djoser) in the landscape of Memphis and Saqqara necropolis as a ritual, cultural and visual reference point. Additionally, an interpretation of its meaning and purpose, including the above- and underground structures as well as the so-called Dry Moat, is presented.
Living Mulch with Subterranean Clover (Trifolium subterraneum L.) Is Effective for a Sustainable Weed Management in Globe Artichoke as Annual Cropping in Puglia (Southern Italy)
Authors: Fracchiolla Mariano , Lasorella Cesare , Cazzato Eugenio , Renna Massimiliano
Published: September 2022

Italy represents the world leading producer of globe artichoke, and Puglia (Southern Italy) supplies about one-third of the nation’s production. In this research, the influence of mulching (both living mulch with subterranean clover and biodegradable mulch film) on both weed infestation and globe artichoke yield in comparison with conventional tillage was evaluated. Two globe artichoke genotypes (Capriccio—hybrid cultivar—and Brindisino—sanitized local variety) were tested in an open field located in Puglia. The following parameters were evaluated: weed infestation, yield and canopy of globe artichoke, and biomass and canopy of subterranean clover. Yield of globe artichoke (on average 16 buds plant−1) was not influenced by soil management although the total weed cover was lower by using conventional tillage. Mean canopy of T. subterraneum was higher under Brindisino (about 65%) in comparison with Capriccio (about 45%). Dry weight was higher in Brindisino (about 12 g m−2) than Capriccio (about 6 m−2) without differences among soil management treatments. Subterranean clover showed a good ability to control weed cover especially under Brindisino genotype (weed infestation always less than 1%) highlighting its particularly suitability for local varieties of globe artichoke instead of hybrid cultivars (weed infestation up to 5%). In conclusion, the results of this study suggest the positive effects of living mulch with subterranean clover for a sustainable weed management in globe artichoke as annual cropping in Puglia.
Factors Influencing Smoking among Multicultural Adolescents
Authors: Park Jin-Hee , Kim Mi-Jin , Kim Sung-Eun
Published: September 2022

Since an increasing number of multicultural adolescents have started smoking in Korean society, it is necessary to check the smoking status among multicultural adolescents and provide differentiated smoking cessation education and awareness through intervention programs. This study examined the factors that influenced smoking among multicultural adolescents and identified interventions. This study was a cross-sectional survey. It used raw data from the 15th Korea Youth Risk Behavior Web-based Survey (KYRBS) of 2019. Of the 57,303 participants in the 15th KYRBS, 749 were multicultural adolescents, i.e., their fathers or mothers were not born in Korea. The factors affecting smoking among multicultural adolescents were determined using a composite sample multiple logistic regression analysis. The results of the composite sample multiple logistic regression analysis revealed that 41 (6.4%) participants were smokers, had low academic performance levels, drank alcohol, were sexually active, and were more likely to smoke with other adolescents. They were 15.79 times more likely to smoke while drinking than when not drinking. Educational and psychological interventions are needed to increase multicultural youth school adaptation and academic performance levels, reduce health risk behaviors (drinking and sexually active), and ultimately, lower the smoking rate.