bluuebunny/crossref_metadata_2025 huggingface dataset:
Can Agroforestry Contribute to Food and Livelihood Security for Indonesia’s Smallholders in the Climate Change Era?
Authors: Sudomo Aris , Leksono Budi , Tata Hesti Lestari , Rahayu Anita Apriliani Dwi , Umroni Aziz , Rianawati Heny , Asmaliyah  , Krisnawati  , Setyayudi Ali , Utomo Marcellinus Mandira Budi , Pieter Levina Augusta Geraldine , Wresta Arini , Indrajaya Yonky , Rahman Syed Ajijur , Baral Himlal
Published: September 2023

In Indonesia, smallholders have historically practiced agroforestry, which warrants examination in terms of food and livelihood security within sustainable community forest frameworks. Based on a literature review, we analyzed these two forms of security related to smallholder agroforestry practices. Findings indicate diverse agroforestry systems, with 88% focusing on non-timber forest products (NTFPs) and 12% on timber. While 42% prioritize direct food supply, 58% emphasize income generation through product sales. However, agroforestry that does not produce food for direct consumption by smallholders generates revenue for purchasing food necessities. Agroforestry supports both food needs (46–61%) and income (51–54%) for smallholders, surpassing traditional agriculture (13%). Semi-commercial agroforestry (57%) is a predominant livelihood prospect. The remaining 27% are purely subsistence, and 15% are purely commercial. However, the commercialization of agroforestry that focuses only on high-value commodities results in a negative impact on biodiversity. There is a concomitant decrease in environmental services for climate change mitigation and adaptation. Biodiversity remains crucial for climate resilience, health care, and food security in rural communities. Semi-commercial agroforestry is a midpoint for achieving multifunctional agriculture (biodiversity, soil and water conservation, food security, and income) in the climate change era. The research directly related to food security and ecosystem services quantification remains limited, necessitating further investigation. Policy support and incentives are essential for smallholders practicing complex agroforestry for climate adaptation and mitigation.
Impact of Preharvest Ethephon Foliar Spray on the Postharvest Fatty Acid Profile and Dietary Indicators of Macadamia Nuts
Authors: Aruwajoye Noluthando Noxolo , Mditshwa Asanda , Magwaza Lembe Samukelo , Ngidi Mjabuliseni Simon Cloapas , Tesfay Samson Zeray
Published: September 2023

The use of ethephon, designed to stimulate nut detachment, initiates the release of ethylene, a well-established regulator of postharvest shelf-life in various agricultural products. This study aimed to assess the impact of ethephon application on individual fatty acids and dietary indicators in two macadamia nut cultivars, namely ‘788’ and ‘Beaumont,’ during postharvest storage. Nuts that naturally abscised and those detached through ethephon treatment were divided into two groups: the ethylene-treated group (ED) and the control group (CD). Nuts were stored at 25 °C and sampled at 0, 36, and 72 days for fatty profile analysis. Our findings indicated a significant increase in stearic acid content in ED nuts (24,622 µg/g) compared to CD nuts (16,764 µg/g) at the end of the storage period for the ‘Beaumont’ cultivar. Additionally, unsaturated fatty acids (USFAs), such as eicosatrienoic acid + erucic acid (C20:3n3 + C22:1) and eicosatrienoic acid + alpha-linolenic acid (C20:1 + C18:3n3), were notably reduced. Hierarchical clustering analysis revealed positive correlations between ethylene treatment and saturated fatty acids (SFAs) in both ‘Beaumont’ (0.78) and ‘788’ (0.80) cultivars. This also coincided with an increase in atherogenic indices, thrombogenic index, and saturation index and a decrease in the hypocholesterolemic/hypercholesterolemic ratio and arachidonic acid (C20:4n6) within the ED group of the ‘Beaumont’ cultivar, collectively potentially impacting nutritional quality negatively. Furthermore, our findings indicated that the PUFA:SFA ratio was higher in CD (0.51) compared to ED (0.45) on day 72 for the ‘Beaumont’ cultivar, revealing differences in fatty acid compositions between the two treatment groups. Conversely, for ‘788’, both ED and CD had a PUFA:SFA ratio below 0.45, indicating an increased risk of cardiovascular diseases. These results suggest that ethephon treatment increases SFA levels and reduces USFA levels in the ‘Beaumont’ cultivar, while the response to ethylene varies between the two cultivars. Thus, the study provides insight into the significant role of modifying ethephon treatment methods and careful cultivar selection in the attainment of optimal nutritional value and shelf-life of macadamia nuts.
Symbiosis—A Perspective on the Effects of Host Traits and Environmental Parameters in Arbuscular Mycorrhizal Fungal Richness, Colonization and Ecological Functions
Authors: Enebe Matthew Chekwube , Erasmus Mariana
Published: September 2023

Arbuscular mycorrhizal fungi are biotrophic fungi that form an association with plant roots and render benefits in nutrient uptake, disease control and plant tolerance to stress conditions. Plant–mycorrhizal fungi interaction has been proposed as a suitable tool for contributing to sustainable agriculture and reducing the dependence on agrochemicals. Interactions between plants and arbuscular mycorrhizal fungi are regulated by several factors ranging from host traits to environmental conditions that affect the species richness, diversity and functions. In this review, we highlight recent advances on how host traits and environmental conditions in farming systems and/or in natural ecosystems affect the richness, physiology and ecological functions of arbuscular mycorrhizal fungi while specifying the gaps that need to be filled through research.
Application of Lavender and Rosemary Essential Oils (EOs), Their Mixture and Eucalyptol (EOs Main Compound) on Cucumber Fruit Quality Attributes and Microbial Load
Authors: Xylia Panayiota , Goumenos Christos , Tzortzakis Nikolaos , Chrysargyris Antonios
Published: September 2023

Cucumber (Cucumis sativus L.), one of the most widely consumed vegetables, presents high perishability during storage and marketing if it is not handled and stored properly. Currently, there is an increased interest of the food industry to reduce waste (due to quality losses) and to utilize natural products for the preservation of fresh commodities. This study’s goal was to evaluate the effects of lavender (Lav) and rosemary (Ros) essential oils (EOs), their mixture (Lav + Ros, 1:1 v/v) and their main compound (eucalyptol) via vapor phase on cucumber’s postharvest quality. The outcomes of this study demonstrated that 200 μL/L of Lav and Ros EOs increased the respiration rate of cucumbers after 10 days of storage at 11 °C, while 100 μL/L of the EOs mixture and Eucalyptol (100 and 200 μL/L) had no effect on respiration, on the same day. The application of Eucalyptol (100 and 200 μL/L) resulted in less acceptable fruits (less pleasant aroma and unpleasant taste). A decrease in fruit firmness was found in cucumbers exposed to Lav 200 μL/L and Ros 100 μL/L. Interestingly, Eucalyptol was found to accelerate the fruit ripening index after five days of storage, and to decrease organoleptic properties of the fruit (i.e., aroma, taste) on the fifth day of storage. The fruit revealed increased oxidative stress (i.e., increased lipid peroxidation), especially at a high concentration (200 μL/L) of Eucalyptol after 10 days. This has resulted in the activation of other non-enzymatic antioxidant mechanisms such as the increase in fruit ascorbic acid content. Notably, no effects on fruit weight loss, total soluble solids and color were observed with the examined treatments. Overall, this study suggests that the investigated products (EOs and their main compound) have a putative role in postharvest storage for the preservation of cucumbers. However, further investigation is needed for the determination of the optimum application conditions (i.e., concentration, time and method of application) on cucumbers and other fresh produce.
Quantifying CO2 Emissions and Carbon Sequestration from Digestate-Amended Soil Using Natural 13C Abundance as a Tracer
Authors: Reuland Gregory , Sleutel Steven , Li Haichao , Dekker Harmen , Sigurnjak Ivona , Meers Erik
Published: September 2023

The untapped potential for carbon sequestration in agricultural soils represents one of the most cost-effective tools for climate change mitigation. Increasing soil organic matter also brings other agronomic benefits such as improved soil structure, enhanced water-and-nutrient-retention capacity, and biological activity. Broadly, soil organic carbon storage is achieved by increasing carbon inputs (plant residues and organic amendments) and reducing carbon outputs (soil loss mechanisms, decomposition). With a focus on carbon inputs—more specifically, organic amendments—as leverage to increase soil organic carbon, we compared the respiration rates and carbon storage of incubated soil cores amended with maize straw, manure, two digestates and the solid fraction of digestate. Using the variation in the natural 13C abundance found in C4 and C3 plants as a tracer, we were able to partition the CO2 emissions between the exogenous organic matter materials elaborated from maize (C4) and native soil organic carbon (C3). The addition of digestate resulted in an additional 65 to 77% of remaining organic carbon after 92 days. The digestate-derived CO2 was fitted to a second-order kinetic carbon model that accounts for the substrate C that is assimilated into the microbial biomass. The model predicted a carbon sequestration potential of 56 to 73% of the total applied organic carbon after one to two years. For the solid fraction, the results were higher, with 89% of the applied organic carbon after 92 days and a sequestration potential of 86%. The soil priming ranged from −19% to +136% in relation to the unamended control soil, highlighting a surprisingly wide spectrum of results that warrants the need for further research on soil–digestate interactions.
Effect of Irrigation Methods on Black Truffle Production
Authors: Magarzo Alba , Alba Sonia , Santos del Blanco Luis , Franco Manchón Iván , Olaizola Jaime , Martín Pinto Pablo , Mediavilla Olaya
Published: September 2023

Spain is one of the main producers of black truffle (Tuber melanosporum Vittad.), a fungus of great economic importance. Black truffles are usually cultivated in Quercus ilex orchards, as water availability is one of the most important factors influencing truffle production. Optimizing watering systems is essential to reduce the amount of water wasted. Nevertheless, up to now, no study has been carried out comparing the efficiency of different irrigation systems in truffle plantations. The aim of this study was to compare the efficiency of two different irrigation systems, namely a drip irrigation system and a micro-sprinkler system, in a Quercus ilex plantation situated in Burgos, Spain. Our data showed that there were no differences between the two irrigation systems in terms of truffle yields, the number of truffles, quality (based on truffle size), or the date of truffle harvesting. However, when other parameters were taken into consideration, such as the economic and environmental impact of installing and running these systems, drip irrigation was deemed the superior irrigation system because it uses less water. This study validates for the first time the use of drip irrigation rather than a micro-sprinkler system (the most commonly used in truffle plantations) because of its greater water use efficiency, which is an increasingly important consideration given future climate change scenarios marked by global water scarcity.
Exploring the Impact of Insertion/Deletion in FTO and PLIN1 Genes on Morphometric Traits in Sheep
Authors: Wang Xinle , Li Jingyun , Bai Junyan , Chen Mengke , Wang Longwei , Fan Hongdeng , Zeng Fanlin , Lu Xiaoning , He Yuhan
Published: September 2023

This study aimed to identify InDels from the FTO and PLIN1 genes and to analyze their association with morphometric traits in Hu sheep (HS), Dupor sheep (DS), and Small Tail Han sheep (STHS). The FTO and PLIN1 genes were genotyped using the insertion/deletion (InDel) method. A one-way ANOVA with SPSS 26.0 software (IBM Corp, Armonk, NY, USA) was used to assess the effect of the InDel FTO and PLIN1 genes on morphometric traits. The results revealed significant associations between certain InDels and the morphometric traits in different breeds of sheep. Specifically, FTO-2 was significantly associated with cannon circumference (CaC) in HS rams and body height (BoH) in HS ewes (p < 0.05). FTO-2 was also significantly associated with chest width (ChW), CaC, head length (HeL), and coccyx length (CoL) in the STHS breed (p < 0.05). FTO-3 showed significant associations with BoH in HS rams and BoH, back height (BaH), ChW, and chest depth (ChD) in HS ewes (p < 0.05). FTO-3 was also significantly associated with ChW in the DS and STHS breeds (p < 0.05). FTO-5 was significantly associated with body weight (BoW) in the DS breed and BoH in the STHS breed (p < 0.05). Furthermore, PLIN1 was significantly related to BoW in the DS breed and was significantly associated with CoL and forehead width (FoW) in the STHS breed (p < 0.05). In conclusion, the study suggested that InDels in the FTO and PLIN1 genes could provide practical information to improve morphometric traits in sheep breeding.
Describing the Pollen Content in the Gastrointestinal Tract of Vespa velutina Larvae
Authors: Diéguez-Antón Ana , Escuredo Olga , Meno Laura , Seijo María Carmen , Rodríguez-Flores María Shantal
Published: September 2023

Vespa velutina is an invasive species that exhibits flexible social behavior, which may have contributed to its introduction in several European countries. It is important to understand its behavior in order to combat the effects of its introduction in different areas. This implies knowing the resources that it uses during its biological cycle. Hornets require protein resources taken from insects and organic matter as well as carbohydrates as an energy source to fly and also to forage for food and nest-building materials. The gastrointestinal tract of adults and larvae contains a wide variety of pollen types. The identification of this pollen in larvae collected from nests could offer information about the plant species that V. velutina visits as a foraging place. The main objective of this research was to study the pollen content in the gastrointestinal tract of larvae. Patterns of pollen content and pollen diversity were established according to the nest type, altitude, season, and location in the nest comb. The abundance of pollen types such as Eucalyptus, Castanea, Foeniculum vulgare, Hedera helix, Taraxacum officinale, Echium, or Cytisus pollen type stands out in many of the samples.
Non-Invasive Quantification of Faecal and Urine Reproductive Hormone Metabolites in the Naked Mole-Rat (Heterocephalus glaber)
Authors: Majelantle Tshepiso Lesedi , Ganswindt Andre , Ganswindt Stefanie Birgit , Hagenah Nicole , Hart Daniel William , Bennett Nigel Charles
Published: September 2023

The naked mole-rat (Heterocephalus glaber) occurs in colonies with a distinct dominance hierarchy, including one dominant, breeding female (the queen), 1–3 breeding males, and non-reproductive subordinates of both sexes that are reproductively suppressed while in the colony. To non-invasively evaluate reproductive capacity in the species, we first had to examine the suitability of enzyme immunoassays (EIAs) for determining progestogen and androgen metabolite concentrations in the naked mole-rat, using urine and faeces. A saline control and gonadotrophin-releasing hormone (GnRH) were administered to twelve (six males and six females) naked mole-rats which were previously identified as dispersers and housed singly. The results revealed that urine is possibly not an ideal matrix for progestogen and androgen metabolite quantification in naked mole-rats as no signal was detected in the matrix post GnRH administration. A 5α-Progesterone EIA and an Epiandrosterone EIA were identified as suitable for quantifying faecal progesterone metabolites (fPMs) and faecal androgen metabolites (fAMs) in males and females, respectively. The results suggest that there are individual variations in baseline fPM and fAM concentrations, and only two out of six females and no males exhibited an increase in fPM concentrations greater than 100% (−20% SD) post GnRH administration. Conversely, only four out of six females and three out of six males had an increase in fAM concentrations greater than 100% (−20% SD) following GnRH administration. These results imply that some naked mole-rat individuals have a reduced reproductive capacity even when they are separated from the queen.
Palm Kernel Meal Protein Hydrolysates Enhance Post-Thawed Boar Sperm Quality
Authors: Khophloiklang Vassakorn , Chanapiwat Panida , Aunpad Ratchaneewan , Kaeoket Kampon
Published: September 2023

Boar sperm is sensitive to particular conditions during cryopreservation, resulting in an extreme reduction in fertilizing ability due to damage to the sperm membranes. PKMPH contains bioactive peptides that have antioxidant and antimicrobial activities. There is no information on the use of palm-kernel-meal-derived bioactive peptides for boar semen cryopreservation. This study aimed to examine the effects of bioactive peptides from PKMPH on post-thawed boar sperm quality. Boar semen ejaculates (n = 17) were collected and divided into six equal aliquots based on PKMPH concentrations (0, 1.25, 2.5, 5, 10, and 15 µg/mL) in a freezing extender. Semen samples were processed and cryopreserved using the liquid nitrogen vapor method. Thereafter, the frozen semen samples were thawed at 50 °C for 12 s and evaluated for sperm motility using a computer-assisted sperm analyzer and for sperm viability, acrosome integrity, mitochondrial function, and lipid peroxidation by measuring the level of malondialdehyde (MDA). The results demonstrate that the supplementation of PKMPH with 2.5 µg/mL afforded superior post-thawed sperm qualities, such as increased total motility, viability, acrosome integrity, and mitochondrial function by 10.7%, 12.3%, 18.3%, and 12.7%, respectively, when compared to the control group. PKMPH at a concentration of 2.5 µg/mL showed the lowest level of MDA (40.6 ± 2.0 µMol/L) compared to the other groups. In conclusion, adding PKMPH peptides at 2.5 µg/mL to the freezing extender reduced the oxidative damage associated with cryopreservation and resulted in higher post-thawed sperm quality.
Climate Spaces and Cliffs: A Novel Bovine Thermodynamic and Mass Balances Model
Authors: Porter Warren P. , Bertz Alexa E. , Mathewson Paul D. , Solorzano Luis C. , Dudley Peter N. , Bonazza Riccardo , Gebremedhin Kifle G.
Published: September 2023

The effects of climate change on animals are typically viewed in terms of survivability and wellbeing. In this study, we broaden that purview to include climate impacts on reproductive capability. There are not only climate spaces for daily function, but climate cliffs that represent reproductive failures in the face of climate warming. This alternative focus suggests that climate warming challenges may be more immediate and profound than initially imagined. This research describes a state-of-the-art mechanistic model, Dairy Niche Mapper (DNM), and independent validation tests. Where test data are absent, the calculated results are consistent with expected responses. Simulations of metabolic chamber conditions reveal the local steady-state impacts of climate and animal variables on milk production capacity, metabolic rate, food consumption and water needs. Simulations of a temperature humidity index (THI) show strengths and limitations of that approach. Broader time- and spatial-scale calculations applied in the western and eastern halves of the northern hemisphere identify current and future monthly latitudinal climate change impacts on milk production potential, feed and water needs in dairy cows of different sizes. Dairy Niche Mapper (DNM) was developed from a broadly tested mechanistic microclimate-animal model, Niche Mapper (NM). DNM provides an improved quantitative understanding of the complex nonlinear interactions of climate variation and dairy bovine properties’ effects on current and future milk production, feed and water needs for grazing and confinement dairy operations. DNM outputs include feasible activity times, milk production and water and feed needs of different-sized Holstein cows on high-grain (confinement feeding) versus high-forage (grazing feeding) diets at three arbitrary north latitudes, 12°, 30° and 60°, for North and Central America and for Asia. These three latitudes encompass current northern hemisphere bovine production environments and possible future production locations. The greatest impacts of climate change will be in the low elevations in tropical and subtropical regions. Global regions above 30° and below 60° latitude with reliable rainfall will be least affected by current projected levels of climate change. This work provides the basis for computational animal design for guiding agricultural development via breeding programs, genetic engineering, management options including siting or the manipulation of other relevant environmental and animal variables.
Evaluation of Interactions of Added Soybean Peroxidase with Other Nutrients Present in Fish Feeds Using an In Vitro Digestive Simulation
Authors: Nogueira Wesclen Vilar , Aznar-García María Jesús , Martínez-Antequera Francisca P. , de Las Heras Antonia M. Barros , Tesser Marcelo Borges , Garda-Buffon Jaqueline , Moyano Francisco Javier
Published: September 2023

Peroxidase (PO) has been applied in different areas of industrial biotechnology, including the control of contaminants like aflatoxin B1 in fish feeds. However, its potential negative interactions with the macro and micro components of feeds have not been evaluated. The aim of this study was to evaluate the impact of PO’s addition to a feed on compounds like fatty acids and polyphenols using an in vitro simulation of the digestive tract of the tilapia. The influence on fatty acids was determined by changes in the peroxide index, with the feed including PO presenting values four times higher than those of the control feed. On the other hand, the in vitro digestive simulation also evidenced an effect of PO on the bioaccessibility of polyphenols significantly influenced by the total digestion time and temperature. The bioaccessibility of polyphenol ranged from 2.09 to 16.23 μmol of the total Trolox equivalent antioxidant capacity for the combinations evaluated in the study. The greatest bioaccessibility was observed at the central point under the following conditions of digestive hydrolysis: pH of 7, 30 °C, 4.5 h of digestive hydrolysis and an absence of PO.
The Role of Companion Dogs in the VA Maryland Health Care System Whole Health(y) GeroFit Program
Authors: Ortmeyer Heidi K. , Giffuni Jamie , Etchberger Danielle , Katzel Leslie
Published: September 2023

GeroFit is a gym-based exercise program that promotes health and wellness among older sedentary veterans. The aims of the current study were to determine whether providing a companion dog as an alternative to gym-based exercise would similarly affect whole health outcomes. A total of 15 (n = 15) veterans (62 ± 11 years of age; 13 of 15 >54 years of age) underwent physical function testing, completed global and whole health questionnaires, and wore an accelerometer for 7 days before (baseline) and 3 months after a dog came into their home. The participants completed the Pet Attachment Scale (PAS), Dog Owner-Specific Quality of Life (DOQOL), and Canine Behavioral Assessment and Research questionnaires at 3 months. Cardiorespiratory endurance, lower body strength, daily steps, and time spent engaging in moderate physical activity all increased compared to the baseline levels. Body weight decreased among veterans whose body mass index was ≥30 (n = 11). The PAS and DOQOL scores indicated high attachment and positive effects on quality of life after having a dog in the home, with all veterans agreeing that having a dog improved the number of social activities they performed. We conclude that providing a companion dog to veterans not inclined to participate in gym-based exercise is an effective alternative method of promoting health and wellness in this population.
New Evidence for the Role of Pituitary Adenylate Cyclase-Activating Polypeptide as an Antimicrobial Peptide in Teleost Fish
Authors: Velázquez Janet , Rodríguez-Cornejo Tania , Rodríguez-Ramos Tania , Pérez-Rodríguez Geysi , Rivera Laura , Campbell James Hugh , Al-Hussinee Lowia , Carpio Yamila , Estrada Mario Pablo , Dixon Brian
Published: September 2023

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) is a multifunctional neuropeptide that is widely distributed and conserved across species. We have previously shown that in teleost fish, PACAP not only possesses direct antimicrobial properties but also immunomodulatory effects against the bacterial pathogens Flavobacterium psychrophilum and Pseudomonas aeruginosa using in vitro and in vivo experiments. These previous results suggest PACAP can be used as an alternative to antibiotics to prevent and/or treat bacterial infections in the aquaculture industry. To accomplish this goal, more studies are needed to better understand the effect of PACAP on pathogens affecting fish in live infections. In the present study, the transcripts PACAP, PRP/PACAP, and VPAC2 receptor were examined in rainbow trout (Oncorhynchus mykiss) naturally infected with Yersinia ruckeri, which exhibited an increase in their expression in the spleen when compared to healthy fish. Synthetic Clarias gariepinus PACAP-38 has direct antimicrobial activity on Y. ruckeri and inhibits up to 60% of the bacterial growth when the peptide is at concentrations between 50 and 100 µM in TSB. The growth inhibition increased up to 90% in the presence of 12.5 µM of PACAP-38 when salt-free LB broth was used instead of TSB. It was also found to inhibit Y. ruckeri growth in a dose-dependent manner when the rainbow trout monocyte/macrophage-like cell line (RTS11) was pre-treated with lower concentrations of the peptide (0.02 and 0.1 µM) before going through infection. Differential gene expression was analyzed in this in vitro model. Overall, the results revealed new evidence to support the role of PACAP as an antimicrobial and immunomodulatory peptide treatment in teleosts.
Periprosthetic Joint Infection Diagnosis: A Narrative Review
Authors: Tsikopoulos Konstantinos , Meroni Gabriele
Published: September 2023

Replacement of native joints aims to restore patients’ quality of life by relieving pain and improving joint function. While periprosthetic joint infection (PJI) affects a small percentage of patients, with an estimated incidence of 1–9% following primary total joint replacement, this postoperative complication necessitates a lengthy hospitalisation, extended antibiotic treatment and further surgery. It is highlighted that establishing the correct diagnosis of periprosthetic infections is critical in order for clinicians to avoid unnecessary treatments in patients with aseptic failure. Of note, the PJI diagnosis could not purely rely upon clinical manifestations given the fact that heterogeneity in host factors (e.g., age and comorbidities), variability in infection period, difference in anatomical location of the involved joint and discrepancies in pathogenicity/virulence of the causative organisms may confound the clinical picture. Furthermore, intra-operative contamination is considered to be the main culprit that can result in early or delayed infection, with the hematogenous spread being the most prevalent mode. To elaborate, early and hematogenous infections often start suddenly, whereas chronic late infections are induced by less virulent bacteria and tend to manifest in a more quiescent manner. Last but not least, viruses and fungal microorganisms exert a role in PJI pathogenesis.
Design Characteristics and Recruitment Rates for Randomized Trials of Peri-Prosthetic Joint Infection Management: A Systematic Review
Authors: Manning Laurens , Allen Bethwyn , Davis Joshua S.
Published: September 2023

Prosthetic joint infections (PJI) present a major management challenge for practicing orthopedic surgeons and infectious disease physicians. There are few high-quality data to inform treatment guidelines. The aim of this systematic review was to report the design characteristics and recruitment rates for randomized controlled trials (RCTs) of PJI management. Trials were considered eligible for inclusion if human participants were randomized to any management intervention for PJI. We searched Medline, PubMed, Embase, Web of Science, Cochrane Database, ANZ Clinical Trials Registry, ClinicalTrials.gov, and the EU Clinical Trials Register until the end of May 2023. The systematic review was registered with PROSPERO (CRD42018112646). We identified 15 published RCTs with a total of 1743 participants with PJI. The median (interquartile range [IQR]) number of successfully recruited participants was 63 (38–140), with 0.28 (0.13–0.96) enrolments per site per month. Only four trials (36.4%) achieved the target recruitment. All RCTs applied different primary endpoints and varying definitions of a ‘good’ outcome. Despite recent improvements, PJI RCTs are characterized by slow recruitment and heterogeneous endpoint assessments, which preclude synthesis in a standard meta-analytic framework. To inform international guidelines, future PJI trials should be run as multi-country trials at high-recruiting sites.
Monoclonal Antibody Disrupts Biofilm Structure and Restores Antibiotic Susceptibility in an Orthopedic Implant Infection Model
Authors: Burke Zachary D. C. , Hart Christopher M. , Kelley Benjamin V. , Mamouei Zeinab , Blumstein Gideon W. , Hamad Christopher , Hori Kellyn , Cevallos Nicolas , Villalpando Christina , Truong Nicole , Turkmani Amr , Ralston Micah , Kavanaugh Aaron , Tenorio Edgar , Kauvar Lawrence M. , Li Alan , Prunet Nathanael , Stavrakis Alexandra I. , Bernthal Nicholas M.
Published: September 2023

Bacterial biofilms on orthopedic implants are resistant to the host immune response and to traditional systemic antibiotics. Novel therapies are needed to improve patient outcomes. TRL1068 is a human monoclonal antibody (mAb) against a biofilm anchoring protein. For assessment of this agent in an orthopedic implant infection model, efficacy was measured by reduction in bacterial burden of Staphylococcus aureus, the most common pathogen for prosthetic joint infections (PJI). Systemic treatment with the biofilm disrupting mAb TRL1068 in conjunction with vancomycin eradicated S. aureus from steel pins implanted in the spine for 26 of 27 mice, significantly more than for vancomycin alone. The mechanism of action was elucidated by two microscopy studies. First, TRL1068 was localized to biofilm using a fluorescent antibody tag. Second, a qualitative effect on biofilm structure was observed using scanning electron microscopy (SEM) to examine steel pins that had been treated in vivo. SEM images of implants retrieved from control mice showed abundant three-dimensional biofilms, whereas those from mice treated with TRL1068 did not. Clinical Significance: TRL1068 binds at high affinity to S. aureus biofilms, thereby disrupting the three-dimensional structure and significantly reducing implant CFUs in a well-characterized orthopedic model for which prior tested agents have shown only partial efficacy. TRL1068 represents a promising systemic treatment for orthopedic implant infection.
Changes in the Prescription of Antibiotics and Phytopharmaceuticals in Children Treated for Acute Upper and Lower Respiratory Tract Infections in Pediatric Practices in Germany in 2013, 2018, and 2022
Authors: Kostev Karel , van den Boom Louisa , Tanislav Christian , Jacob Louis
Published: September 2023

Background: Little is known about the recent trends in antibiotic and phytopharmaceutical prescribing for acute upper (URIs) and lower respiratory tract infections (LRIs) in children and adolescents. Therefore, this study investigated changes in the prescription of antibiotics and phytopharmaceuticals in children diagnosed with acute URIs and LRIs in pediatric practices in Germany in 2013, 2018, and 2022. Methods: The present retrospective study included children aged 2–12 years diagnosed with acute URIs or LRIs in one of 180 pediatric practices in 2013, 2018, and 2022. The URIs included nasopharyngitis, sinusitis, pharyngitis, tonsillitis, laryngitis and tracheitis, and upper respiratory infections of multiple and unspecified sites, while the LRIs corresponded to bronchitis. The primary outcomes were the proportion of children being prescribed antibiotics and the proportion of those being prescribed phytopharmaceuticals. Results: A total of 120,894 children were diagnosed with acute URIs or LRIs in 2013 compared to 116,844 in 2018 and 127,821 in 2022. The prevalence of antibiotic prescription decreased for all diagnoses between 2013 and 2022. This decrease was statistically significant for both 2013–2018 and 2018–2022 for nasopharyngitis, pharyngitis, and bronchitis. Meanwhile, there was a significant increase in the use of phytopharmaceuticals for all diagnoses between 2013 and 2018. The prevalence of phytopharmaceutical prescription decreased slightly between 2018 and 2022, but this decrease was generally not statistically significant. Conclusions: The prescription of antibiotics has decreased and that of phytopharmaceuticals has increased in children diagnosed with acute URIs and LRIs in Germany over the last decade. More data are needed to corroborate these findings in other settings.
Altered Lipid Moieties and Carbonyls in a Wistar Rat Dietary Model of Subclinical Fatty Liver: Potential Sex-Specific Biomarkers of Early Fatty Liver Disease?
Authors: Martín-Grau María , Pardo-Tendero Mercedes , Casanova Pilar , Dromant Mar , Marrachelli Vannina G. , Morales Jose Manuel , Borrás Consuelo , Pisoni Serena , Maestrini Sabrina , Di Blasio Anna M. , Monleon Daniel
Published: September 2023

Non-alcoholic fatty liver disease (NAFLD) is a condition in which excess fat builds up in the liver. To date, there is a lack of knowledge about the subtype of lipid structures affected in the early stages of NAFLD. The aim of this study was to analyze serum and liver lipid moieties, specifically unsaturations and carbonyls, by nuclear magnetic resonance (NMR) in a subclinical Wistar rat model of NAFLD for detecting early alterations and potential sex dimorphisms. Twelve weeks of a high-fat diet (HFD) induced fat accumulation in the liver to a similar extent in male and female Wistar rats. In addition to total liver fat accumulation, Wistar rats showed a shift in lipid subtype composition. HFD rats displayed increased lipid carbonyls in both liver and serum, and decreased in unsaturated fatty acids (UFAs) and polyunsaturated fatty acids (PUFAs), with a much stronger effect in male than female animals. Our results revealed that the change in fat was not only quantitative but also qualitative, with dramatic shifts in relevant lipid structures. Finally, we compared the results found in Wistar rats with an analysis in a human patient cohort of extreme obesity. For the first time to our knowledge, lipid carbonyl levels and lipoproteins profiles were analyzed in the context of subclinical NAFLD. The association found between lipid carbonyls and alanine aminotransferase (ALT) in a human cohort of extremely obese individuals further supports the potential role of lipid moieties as biomarkers of early NAFLD.
A Generic Framework for Enhancing Autonomous Driving Accuracy through Multimodal Data Fusion
Authors: Ignatious Henry Alexander , El-Sayed Hesham , Khan Manzoor Ahmed , Kulkarni Parag
Published: September 2023

Higher-level autonomous driving necessitates the best possible execution of important moves under all conditions. Most of the accidents in recent years caused by the AVs launched by leading automobile manufacturers are due to inadequate decision-making, which is a result of their poor perceivance of environmental information. In today’s technology-bound scenarios, versatile sensors are used by AVs to collect environmental information. Due to various technical and natural calamities, the environmental information acquired by the sensors may not be complete and clear, due to which the AVs may misinterpret the information in a different context, leading to inadequate decision-making, which may then lead to fatal accidents. To overcome this drawback, effective preprocessing of raw sensory data is a mandatory task. Pre-processing the sensory data involves two vital tasks, namely data cleaning and data fusion. Since the raw sensory data are complex and exhibit multimodal characteristics, more emphasis is given to data preprocessing. Since more innovative models have been proposed for data cleaning, this study focused on data fusion. In particular, this study proposed a generic data fusion engine, which classifies different formats of sensory data and fuses them accordingly to improve accuracy. This study proposed a generic framework to fuse the text, image, and audio data. In the first stage of this research, an innovative hybrid model was proposed to fuse multispectral image and video data. Simple and efficient models to extract the salient image features were also proposed. The hybrid image fusion model that was proposed did not yield satisfactory outcomes when combining 3D point cloud data, and its performance declined when evaluating large datasets. To address this issue, the study expanded by introducing an advanced generative adversarial network (GAN) to transform the hybrid image fusion model into a machine learning model capable of handling substantial datasets. Additionally, customized kernel functions were suggested to fuse 3D point cloud data effectively. The performance of the proposed models was assessed using standard metrics and datasets, comparing them with existing popular models. The results revealed that the proposed image fusion model outperformed the other models.
Uncertainty Quantification for Thermodynamic Simulations with High-Dimensional Input Spaces Using Sparse Polynomial Chaos Expansion: Retrofit of a Large Thermal Power Plant
Authors: De Meulenaere Roeland , Coppitters Diederik , Sikkema Ale , Maertens Tim , Blondeau Julien
Published: September 2023

The assessment of the future thermodynamics performance of a retrofitted heat and power production unit is prone to many uncertainties due to the large number of parameters involved in the modeling of all its components. To carry out uncertainty quantification analysis, alternatives to the traditional Monte Carlo method must be used due to the large stochastic dimension of the problem. In this paper, sparse polynomial chaos expansion (SPCE) is applied to the retrofit of a large coal-fired power plant into a biomass-fired combined heat and power unit to quantify the main drivers and the overall uncertainty on the plant’s performance. The thermodynamic model encompasses over 180 components and 1500 parameters. A methodology combining the use of SPCE and expert judgment is proposed to narrow down the sources of uncertainty and deliver reliable probability distributions for the main key performance indicators (KPIs). The impact of the uncertainties on each input parameter vary with the considered KPI and its assessment through the computation of Sobol’ indices. For both coal and biomass operations, the most impactful input parameters are the composition of the fuel and its heating value. The uncertainty on the performance and steam quality parameters is not much affected by the retrofit. Key furnace parameters exhibit a skewed probability distribution with large uncertainties, which is a strong attention point in terms of boiler operation and maintenance.
Comparative Study of the Anticancer Effects of Selenium Nanoparticles and Selenium Nanorods: Regulation of Ca2+ Signaling, ER Stress and Apoptosis
Authors: Varlamova Elena G. , Baimler Ilya V. , Gudkov Sergey V. , Turovsky Egor A.
Published: September 2023

The anti-cancer effects of selenium sources are well known. Among other things, selenium has been shown to have a pleiotropic effect, causing cancer cell death without affecting the healthy cell’s viability, or, in the case of brain cells, has a cytoprotective effect. This feature of selenium determined its use in medicine and its use as part of dietary supplements. In recent years, selenium in the form of nanoparticles has received increased attention. Selenium nanoparticles also have anti-cancer effects, and their use appears to be more effective at significantly lower doses compared to other sources of selenium. The shape and size of nanoparticles largely affect the efficiency of nanoselenium application. We obtained two different types of selenium nanoparticles via the laser ablation technique—spherical selenium nanoparticles (SeNPs) about 100 nm in diameter and grown selenium nanorods (SeNrs) about 1 μm long and about 100 nm thick. We compared the anti-cancer efficacy of these two types of selenium nanoparticles using inhibitory analysis, PCR analysis and fluorescence microscopy. It turned out that both types of nanoparticles with high efficiency dose-dependently activate apoptosis in the human glioblastoma cell line A-172, as the most aggressive type of brain tumor. Apoptosis induction was determined not only by the concentration of nanoparticles, but also by the time. It was shown that SeNrs induce the process of apoptosis in glioblastoma cells more efficiently during 24 h of exposure and their effect is enhanced after 48 h without activation of necrosis, whereas the use of spherical SeNPs after 48 h of exposure can cause necrosis in some glioblastoma cells. It has been shown that Ca2+ signals of glioblastoma cells are significantly different for SeNPs and SeNrs. SeNPs cause a dose-dependent transient increase in the number of Ca2+ ions in the cell cytosol ([Ca2+]i), while SeNrs cause a slow rise in [Ca2+]i reaching a new stationary level, which may determine the cytotoxic effects of nanoparticles. It turned out that SeNPs and SeNrs cause depletion of the Ca2+ depot of the endoplasmic reticulum and ER-stress, which correlates with increased expression of genes encoding proapoptotic proteins. In our study, it was found that SeNPs do not activate the Ca2+ signaling system of healthy L-929 mouse fibroblast cells, while SeNrs activate a moderate slow growth in [Ca2+]i. That fact could indicate a lower selectivity of the SeNrs action.
The Use of Chlorophyll Fluorescence as an Indicator of Predicting Potato Yield, Its Dry Matter and Starch in the Conditions of Using Microbiological Preparations
Authors: Pszczółkowski Piotr , Sawicka Barbara , Skiba Dominika , Barbaś Piotr , Noaema Ali Hulail
Published: September 2023

The paradigm shift toward ecological agriculture has spurred efforts to phase out the use of conventional pesticides, and researchers are actively seeking natural alternatives to replace these chemicals. Aim: This study aimed to introduce an innovative intervention to enhance potato yields in a non-invasive manner, thereby elevating the content of both dry matter and starch. Ultimately, this approach seeks to enhance the quality of raw materials destined for the production of potato-enriched products. A field experiment was conducted in central-eastern Poland that employed a randomized sub-block method within a dependent split-split-plot design replicated three times. The main factor was pre-planting treatments, which included the application of a microbiological preparation to seed potatoes for: (a) 10 min, (b) 15 min, and (c) no treatment (control). Another factor was the fourteen potato cultivars of different earliness groups. Qualitative analyses were carried out in laboratory conditions. The application of the microbiological preparation yielded positive outcomes on the physiological indicators of potato yield, while augmenting the production of dry matter and starch when compared to the control object. The analyzed cultivars had the most pronounced impact on both the content and yield of dry matter and starch, demonstrating a wide array of responses to pre-planting treatments that incorporated microbiological preparations. For the first time in studies concerning effective microorganisms, chlorophyll fluorescence analysis was employed. Alternative solutions in the form of employing microbiological preparations offer the potential to effectively substitute pesticides and synthetic fertilizers in potato production, consequently enhancing the quality of raw materials for food.
New Technologies in Geo-Hazards Analysis, Hydrogeology and River Restoration
Authors: Arnaud-Fassetta Gilles
Published: September 2023

Strong societal pressures are now seeking to restore balance to environments that have been destabilized by many years of poor practices in valley bottoms, geological subsoil and slopes [...]
Methods and Applications of Data Mining in Business Domains
Authors: Amrit Chintan , Abdi Asad
Published: September 2023

This Special Issue invited researchers to contribute original research in the field of data mining, particularly in its application to diverse domains, like healthcare, software development, logistics, and human resources [...]
Detection, Localisation and Quantification of Structural Damage Using Changes in Modal Characteristics
Authors: Páleník Marek , Musil Miloš , Úradníček Juraj
Published: September 2023

The article defines and explains methods for detecting and locating damage and quantifying its extent on an example of a finite element model of a simple beam. The defined methods are based on a comparison of the decreases in the bending natural frequencies of the nominally damaged models against the intact model. This comparison assigns natural frequency decreases to curvature of a specific mode shape, which allows creation of an analytical reference model using polynomial regression, which assigns relative natural frequency decreases to the position on the beam. The localisation method assumes that the vector of the relative natural frequency decrease ratios are unique for each position on the beam. The quantification method considers as a relevant comparative quantity, slopes of relative natural frequency decreases, as a function of damage extent. Examples of damage localisation and quantification are defined, and the relative errors of these methods are analysed.
Groundwater Vulnerability Assessment and Protection Strategy in the Coastal Area of China: A GIS-Based DRASTIC Model Approach
Authors: Zhang Qian , Shan Qiang , Chen Feiwu , Liu Junqiu , Yuan Yingwei
Published: September 2023

Groundwater vulnerability reflects the risk level of groundwater contamination and its self-repairing ability, as well as its sustainability for use. Therefore, it provides significant scientific support for implementing measures to prevent groundwater contamination, especially in coastal areas. In this study, considering the lithology of vadose in valley plains and the extent of karst subsidence areas, a GIS-based DRASTIC model was employed to assess groundwater vulnerability in Tangshan City, a coastal area in China. The assessment results were presented and mapped using GIS, based on a comprehensive evaluation of seven parameters, including “Depth of groundwater, Vertical net recharge, Aquifer thickness, Soil media, Topography, Impact of vadose zone, and Hydraulic conductivity”. The identified groundwater vulnerability zones included the highest, higher, moderate, low vulnerability those four zones, which accounted for 4%, 53%, 25%, and 18%, respectively. In addition, according to the results of field investigation, the karst subsidence area and the mined-out coastal area were directly classified as the highest vulnerable areas and covered 1.463 km2; more attention is required here in subsequent groundwater protection processes and strategies. Finally, the groundwater pollution index was used to validate the groundwater vulnerability distribution results, and these two were in high agreement, with an R2 coefficient of 0.961. The study is crucial for the rational utilization and protection of water resources in Tangshan City.