bluuebunny/crossref_metadata_2025 huggingface dataset:
Children, parents and Respiratory Syncytial Virus in Palermo, Italy: prevention is primary
Authors: Di Carlo Paola , Romano Amelia , Plano Maria Rosa Anna , Gueli Alessandra , Scarlata Francesco , Mammina Caterina
Published: April 2010

A study was conducted to describe the characteristics of the Respiratory Syncytial Virus (RSV) infection cases occurring in the season 2006—7 in Palermo, Italy, and to evaluate the parents’ knowledge and behaviours concerning prevention and control of acute respiratory infections (ARIs). All children aged between 0 and 2 years, admitted for a lower respiratory tract infection (LRTI) between October 2006 and May 2007, were enrolled in the study. Data were collected about demographic and household characteristics. Furthermore, their parents were asked to compile a structured questionnaire on transmission, prevention and management of ARIs in children. A total of 198 children with a diagnosis of LRTI were enrolled. Ninety-eight (62.0%) of 157 were positive for RSV. Parents were generally aware of transmission of ARIs through sneezing and/or coughing, but less through contaminated objects or hands. Nationality, age and education level of parents and also the age of the patients proved to be associated with some self-reported knowledge and behaviours. Only 24 (12.3%) of the 195 respondents had received advice from GPs or paediatricians about good hygiene practices. It seems essential to implement public health interventions promoting behavioural changes aimed at the primary prevention of ARIs at the community level.
Food stories: consumption in an age of anxiety
Authors: Jackson Peter
Published: April 2010

This paper (originally presented as the Cultural Geographies 2009 annual lecture) reflects on the agenda set out in Maps of meaning 20 years ago which sought to chart a series of new directions in cultural geography. At the core of that project was an argument about the politics of culture and the idea of cultural politics. Today, that agenda might be re-cast as an attempt to map the intersection of culture and economy or, more precisely, of political and moral economies.This paper will apply these ideas to the geographies of food where, despite relative affluence and food security, consumers in the West (and particularly in Britain) are often described as living through an ‘age of anxiety’. Consumer anxieties about food have been provoked by a range of food scares and farming crises. As a result, manufacturers and retailers have struggled to re-establish consumer trust in food and governments have urged a re-connection between food producers and consumers. The paper explores the concept of consumer anxiety and draws on a series of consumer interviews and life histories with people in the British food industry to illustrate the nature of contemporary anxieties about food. The paper concludes with some reflections on the intersection of public and private anxieties as illustrated by these personal and corporate food stories.
Morale and the affective geographies of the ‘war on terror’
Authors: Anderson Ben
Published: April 2010

This paper focuses on the relations between affect and military and security geographies. It argues that recent work on spaces of affect and emotion should be supplemented by research on how affects are known, rendered actionable and intervened on. The paper exemplifies such a focus through an example of the targeting of amorphous collective affects — specifically morale and popular support — in the ‘effects based’ airpower that has been so central to the US led ‘war on terror’. Damaging or destroying morale has come to promise victory over a networked enemy. Yet morale is assumed to be distributed throughout life. As such, morale disappears as a definite, determinate, thing in itself. It cannot be fixed and located. Consequently, all of life is made into a potential target. To intervene on morale, airpower must create environments that will bring death and create life. The means for doing so are providential and catastrophic biopolitical ‘effects’, of which ‘shock’ and ‘awe’ are but the most high profile cases. Through the example of how morale is targeted, the paper shows how certain forms of power work by being both inside and outside the aleatory, indeterminate, dynamics of affective life.
A secret reservoir of values: the narrative economy of Angel Island Immigration Station
Authors: Hoskins Gareth
Published: April 2010

An interest in narrative has done much to shed light on our understandings of geography. Studies linking narrative to nation building, the making of place, identity, the region, the spaces of health, heritage, and environmental history, give some indication of the breadth at which geographical scholarship has been pushed forward by an applied interest in stories. This article attempts to develop such work with a particular focus on the performative capacities of narrative; how stories might work towards various recuperative outcomes. It discusses the revision of historic tours around Angel Island Immigration Station, a California State Park property and National Historic Landmark with reference to the term narrative economy. The Immigration Station plays host to a narrative economy where stories circulating around the site acquire value on the basis of their factual content and their compatibility with a set of approved messages. Some of these stories are disputed and devalued so as to distinguish them from factual ‘histories’ produced by recently commissioned research. The article considers how heritage sites negotiate tensions between the burden of representational accuracy and the need to function more broadly as platforms for liberatory intervention.
Moving image methodologies for more-than-human geographies
Authors: Lorimer Jamie
Published: April 2010

There is a growing interest in cultural geography in the potential of moving imagery and moving image methodologies for grasping the more-than-human and non-representational dimensions of life. This paper explores this potential to develop moving image methodologies for witnessing and evoking human-nonhuman interactions. Drawing on recent work in film theory, anthropology and ethology, it develops both a practical methodology and a critical, affirmative vocabulary for unpacking the work done by circulating imagery and engaging with its micropolitical power and promise. This analysis is illustrated through a focus on elephants and images of their behaviours, ecologies and interactions with diverse humans. It outlines how video techniques can be used to witness and make sense of elephant encounters. It then maps and compares four of the many affective logics according to which elephants are evoked in popular moving imagery. It reflects on the techniques and the micropolitics of such evocations, before examining what they offer for new ways of engaging with nonhuman difference. Elephants provide an accessible, popular and telegenic nonhuman case study.
Appraising Quantitative Research in Health Education: Guidelines for Public Health Educators
Authors: Jack Leonard , Hayes Sandra C. , Scharalda Jeanfreau G. , Stetson Barbara , Jones-Jack Nkenge H. , Valliere Matthew , Kirchain William R. , LeBlanc Cris
Published: April 2010

Many practicing health educators do not feel they possess the skills necessary to critically appraise quantitative research. This publication is designed to help provide practicing health educators with basic tools helpful to facilitate a better understanding of quantitative research. This article describes the major components— title, introduction, methods, analyses, results, and discussion sections—of quantitative research. Readers will be introduced to information on the various types of study designs and seven key questions health educators can use to facilitate the appraisal process. On reading, health educators will be in a better position to determine whether research studies are well designed and executed.
YouTube versus the National Film and Sound Archive: Which Is the More Useful Resource for Historians of Australian Television?
Authors: McKee Alan
Published: April 2010

This article compares YouTube and the National Film and Sound Archive (NFSA) as resources for television historians interested in viewing old Australian television programs. The author searched for seventeen important television programs, identified in a previous research project, to compare what was available in the two archives and how easy it was to find. The analysis focused on differences in curatorial practices of accessioning and cataloguing. NFSA is stronger in current affairs and older programs, while YouTube is stronger in game shows and lifestyle programs. YouTube is stronger than the NFSA on “human interest” material—births, marriages, and deaths. YouTube accessioning more strongly accords with popular histories of Australian television. Both NFSA and YouTube offer complete episodes of programs, while YouTube also offers many short clips of “moments.” YouTube has more surprising pieces of rare ephemera. YouTube cataloguing is more reliable than that of the NFSA, with fewer broken links. The YouTube metadata can be searched more intuitively. The NFSA generally provides more useful reference information about production and broadcast dates.
Motivation and Selection Processes in a Biographical Transition: A Psychological Mixed Methods Study on the Transition Into Fatherhood
Authors: von der Lippe Holger
Published: April 2010

This article addresses the question of how developmental psychology could benefit from mixed methods research to better explain individual differences in biographical transitions. An event history analysis of 117 young men’s transitions to first-time parenthood is integrated with a qualitative analysis of in-depth interviews on parenthood aspirations that were conducted with 12 childless participants from the same survey. The juxtaposition of both (sub)sampling and results shows, on the one hand, that men’s causal motivated actions to enter into fatherhood can be concluded by combining both qualitative and quantitative interpretations. On the other hand, causal effects on the transition to first-time fatherhood due to partnership selectivity can be concluded from quantitative factors that do not have a qualitative equivalent.
Equality and Equivocation: Saving Sovereignty from Itself
Authors: Stauffer Jill
Published: April 2010

Law and sovereignty are equivocal terms with competing definitions. Sovereignty can refer to coercive state power or a human being’s right to self-determination. In international law sovereignty usually means legalized state autonomy, but that definition is misleading, as state power relies either on domination or cooperation in order to “feel” like sovereignty. Agamben’s analysis of sovereignty as the “nomos of the west” gives us the hard case against saying sovereignty can stand also for human self-determination. But his definitions of law and sovereignty are too narrow to sustain his theory. Constable’s meditation on the silence of justice in modern law counters Agamben, and demonstrates why sovereignty, despite its ties to coercive state power, still holds out resources of freedom and self-determination that no one can afford to forego.
The Elvis We Deserve: The Social Regulation of Sex/ Gender and Sexuality Through Cultural Representations of ‘The King’
Authors: Cowan Sharon
Published: April 2010

This paper analyses the way in which the image, masculinity and sexual identity of Elvis Presley have been recently culturally deployed by particular social groups. It explores the way in which the image of Elvis is used by lesbian drag king performers who try to queer the cultural stereotypes which underpin the social regulation of gender roles; and the use of Elvis’s image by the U.K. fathers’ rights campaign group Fathers 4 Justice, as a sign of unthreatening familiarity to support traditional heteronormative ideas of masculinity and gender roles. These cultural re-appropriations of Elvis raise questions for contemporary understandings of sex/gender and sexuality; as the motto of the San Francisco-based Elvis impersonator ‘‘Extreme Elvis’’ suggests, “Every generation gets the Elvis it deserves.”
Non-invasive management of primary phosphodiesterase type 5 inhibitor failure in patients with erectile dysfunction
Authors: Lowe Gregory , Bahnson Robert
Published: April 2010

Phosphodiesterase type 5 inhibitors (PDE5-i) have become first line therapy for the treatment of erectile dysfunction. Most initial prescriptions for PDE5-i are by primary care practitioners. Urologists must now routinely manage the patient who has failed initial therapy with PDE5-i. Lifestyle modifications can be of benefit to patients. Patient education and optimization of the PDE5-i can result in a successful response. Interestingly, there are reports of up to 60% salvage after changing the PDE5-i utilized. Daily PDE5-i have shown benefit, and treatment of hypogonadism can enhance response to PDE5-i. We review the management of PDE5-i failures with emphasis on noninvasive approaches to gaining improved erectile response to these medications. An algorithm based on the reviewed strategies is proposed to guide clinicians in the treatment of erectile dysfunction.
Experiences and perceptions of secondary food hygiene training: A preliminary study of five larger catering companies in south east Wales
Authors: Worsfold Denise , Griffith Christopher
Published: April 2010

Aims: Food safety training has a high UK profile, although this is mainly concerned with initial training at levels 1—4. Regular secondary hygiene training (collectively refresher, remedial, update training or retraining) for food handlers is recommended by the Catering Industry Guide, although the subject is relatively understudied. The aim of the present work was to determine the beliefs, mode and experiences of secondary training, especially refresher training, in five larger south east Wales catering businesses.

Methods: Construction, piloting and distribution of a questionnaire ( n = 100) followed by descriptive statistical analysis.

Results: A 70% response rate was obtained covering five large catering operations. All had received secondary hygiene training. This training did not appear to be directly related to specific work performance. The duration of this training ranged from 20 minutes to six hours and the frequency from six months to every three years. The majority (94%) agreed that regular secondary training was beneficial and that it could be applied in their work (81%). The content of secondary training varied from a repeat of the initial hygiene course to selected topics deemed relevant to the needs of the employees and the businesses. The majority of refresher training was delivered by conventional classroom instruction but some businesses made use of more informal methods. Trainees estimated that they have only forgotten 10% of their initial training, 63% believed that the best way to refresh their knowledge was to repeat the course and 94% welcomed new information. Most (77%) felt that this training should be tested/examined. Reaction evaluations of secondary training were collected by most businesses and training records were maintained by all.

Conclusions: Secondary training was valued by food handlers. The content and methods of delivering secondary training were more varied and were relatively conservative in format and approach.
Redox regulation of T-cell turnover by the p13 protein of human T-cell leukemia virus type 1: distinct effects in primary versus transformed cells
Authors: Silic-Benussi Micol , Cavallari Ilaria , Vajente Nicola , Vidali Silvia , Chieco-Bianchi Luigi , Di Lisa Fabio , Saggioro Daniela , D'Agostino Donna M. , Ciminale Vincenzo
Published: April 2010

The present study investigated the function of p13, a mitochondrial protein of human T-cell leukemia virus type 1 (HTLV-1). Although necessary for viral propagation in vivo, the mechanism of function of p13 is incompletely understood. Drawing from studies in isolated mitochondria, we analyzed the effects of p13 on mitochondrial reactive oxygen species (ROS) in transformed and primary T cells. In transformed cells (Jurkat, HeLa), p13 did not affect ROS unless the cells were subjected to glucose deprivation, which led to a p13-dependent increase in ROS and cell death. Using RNA interference we confirmed that expression of p13 also influences glucose starvation-induced cell death in the context of HTLV-1–infected cells. ROS measurements showed an increasing gradient from resting to mitogen-activated primary T cells to transformed T cells (Jurkat). Expression of p13 in primary T cells resulted in their activation, an effect that was abrogated by ROS scavengers. These findings suggest that p13 may have a distinct impact on cell turnover depending on the inherent ROS levels; in the context of the HTLV-1 propagation strategy, p13 could increase the pool of “normal” infected cells while culling cells acquiring a transformed phenotype, thus favoring lifelong persistence of the virus in the host.
Elevated IL-17 produced by Th17 cells promotes myeloma cell growth and inhibits immune function in multiple myeloma
Authors: Prabhala Rao H. , Pelluru Dheeraj , Fulciniti Mariateresa , Prabhala Harsha K. , Nanjappa Puru , Song Weihua , Pai Christine , Amin Samir , Tai Yu-Tzu , Richardson Paul G. , Ghobrial Irene M. , Treon Steven P. , Daley John F. , Anderson Kenneth C. , Kutok Jeffery L. , Munshi Nikhil C.
Published: April 2010

Elevated cytokines in bone marrow (BM) micro-environment (interleukin-6 [IL-6], transforming growth factor-beta [TGF-β], and IL-1β) may play an important role in observed immune dysfunction in multiple myeloma (MM). As IL-6 and TGF-β are important for the generation of T-helper 17 (TH17) cells, we evaluated and observed a significantly elevated baseline and induced frequency of Th17 cells in peripheral blood mononuclear cells (PBMCs) and BM mononuclear cells (BMMCs) from MM patients compared with healthy donors. We observed significant increase in levels of serum IL-17, IL-21, IL-22, and IL-23 in blood and BM in MM compared with healthy donors. We also observed that myeloma PBMCs after TH17 polarization significantly induced IL-1α, IL-13, IL-17, and IL-23 production compared with healthy donor PBMCs. We next observed that IL-17 promotes myeloma cell growth and colony formation via IL-17 receptor, adhesion to bone marrow stromal cells (BMSCs) as well as increased growth in vivo in murine xenograft model of human MM. Additionally, we have observed that combination of IL-17 and IL-22 significantly inhibited the production of TH1-mediated cytokines, including interferon-γ (IFN-γ), by healthy donor PBMCs. In conclusion, IL-17–producing Th17 cells play an important role in MM pathobiology and may be an important therapeutic target for anti-MM activity and to improve immune function.
Characterization of Fabry mice treated with recombinant adeno-associated virus 2/8-mediated gene transfer
Authors: Choi Jin-Ok , Lee Mi Hee , Park Hae-Young , Jung Sung-Chul
Published: April 2010

Background

Enzyme replacement therapy (ERT) with α-galactosidase A (α-Gal A) is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease. However, ERT has limitations of a short half-life, requirement for frequent administration, and limited efficacy for patients with renal failure. Therefore, we investigated the efficacy of recombinant adeno-associated virus (rAAV) vector-mediated gene therapy for a Fabry disease mouse model and compared it with that of ERT.

Methods

A pseudotyped rAAV2/8 vector encoding α-Gal A cDNA (rAAV2/8-hAGA) was prepared and injected into 18-week-old male Fabry mice through the tail vein. The α-Gal A expression level and globotriaosylceramide (Gb3) levels in the Fabry mice were examined and compared with Fabry mice with ERT. Immunohistochemical and ultrastructural studies were conducted.

Results

Treatment of Fabry mice with rAAV2/8-hAGA resulted in the clearance of accumulated Gb3 in tissues such as liver, spleen, kidney, heart, and brain with concomitant elevation of α-Gal A enzyme activity. Enzyme activity was elevated for up to 60 weeks. In addition, expression of the α-Gal A protein was identified in the presence of rAAV2/8-hAGA at 6, 12, and 24 weeks after treatment. α-Gal A activity was significantly higher in the mice treated with rAAV2/8-hAGA than in Fabry mice that received ERT. Along with higher α-Gal A activity in the kidney of the Fabry mice treated with gene therapy, immunohistochemical studies showed more α-Gal A expression in the proximal tubules and glomerulus, and less Gb3 deposition in Fabry mice treated with this gene therapy than in mice given ERT. The α-gal A gene transfer significantly reduced the accumulation of Gb3 in the tubules and podocytes of the kidney. Electron microscopic analysis of the kidneys of Fabry mice also showed that gene therapy was more effective than ERT.

Conclusions

The rAAV2/8-hAGA mediated α-Gal A gene therapy provided improved efficiency over ERT in the Fabry disease mouse model. Furthermore, rAAV2/8-hAGA-mediated expression showed a greater effect in the kidney than ERT.
Functional neural differentiation of human adipose tissue-derived stem cells using bFGF and forskolin
Authors: Jang Sujeong , Cho Hyong-Ho , Cho Yong-Bum , Park Jong-Seong , Jeong Han-Seong
Published: April 2010

Background

Adult mesenchymal stem cells (MSCs) derived from adipose tissue have the capacity to differentiate into mesenchymal as well as endodermal and ectodermal cell lineage in vitro. We characterized the multipotent ability of human adipose tissue-derived stem cells (hADSCs) as MSCs and investigated the neural differentiation potential of these cells.

Results

Human ADSCs from earlobe fat maintained self-renewing capacity and differentiated into adipocytes, osteoblasts, or chondrocytes under specific culture conditions. Following neural induction with bFGF and forskolin, hADSCs were differentiated into various types of neural cells including neurons and glia in vitro. In neural differentiated-hADSCs (NI-hADSCs), the immunoreactivities for neural stem cell marker (nestin), neuronal markers (Tuj1, MAP2, NFL, NFM, NFH, NSE, and NeuN), astrocyte marker (GFAP), and oligodendrocyte marker (CNPase) were significantly increased than in the primary hADSCs. RT-PCR analysis demonstrated that the mRNA levels encoding for ABCG2, nestin, Tuj1, MAP2, NFL, NFM, NSE, GAP43, SNAP25, GFAP, and CNPase were also highly increased in NI-hADSCs. Moreover, NI-hADSCs acquired neuron-like functions characterized by the display of voltage-dependent tetrodotoxin (TTX)-sensitive sodium currents, outward potassium currents, and prominent negative resting membrane potentials under whole-cell patch clamp recordings. Further examination by RT-PCR showed that NI-hADSCs expressed high level of ionic channel genes for sodium (SCN5A), potassium (MaxiK, Kv4.2, and EAG2), and calcium channels (CACNA1C and CACNA1G), which were expressed constitutively in the primary hADSCs. In addition, we demonstrated that Kv4.3 and Eag1, potassium channel genes, and NE-Na, a TTX-sensitive sodium channel gene, were highly induced following neural differentiation.

Conclusions

These combined results indicate that hADSCs have the same self-renewing capacity and multipotency as stem cells, and can be differentiated into functional neurons using bFGF and forskolin.
Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression
Authors: Nailis Heleen , Kucharíková Soňa , Řičicová Markéta , Van Dijck Patrick , Deforce Dieter , Nelis Hans , Coenye Tom
Published: April 2010

Background

Candida albicans infections are often associated with biofilm formation. Previous work demonstrated that the expression of HWP1 (hyphal wall protein) and of genes belonging to the ALS (agglutinin-like sequence), SAP (secreted aspartyl protease), PLB (phospholipase B) and LIP (lipase) gene families is associated with biofilm growth on mucosal surfaces. We investigated using real-time PCR whether genes encoding potential virulence factors are also highly expressed in biofilms associated with abiotic surfaces. For this, C. albicans biofilms were grown on silicone in microtiter plates (MTP) or in the Centres for Disease Control (CDC) reactor, on polyurethane in an in vivo subcutaneous catheter rat (SCR) model, and on mucosal surfaces in the reconstituted human epithelium (RHE) model.

Results

HWP1 and genes belonging to the ALS, SAP, PLB and LIP gene families were constitutively expressed in C. albicans biofilms. ALS1-5 were upregulated in all model systems, while ALS9 was mostly downregulated. ALS6 and HWP1 were overexpressed in all models except in the RHE and MTP, respectively. The expression levels of SAP1 were more pronounced in both in vitro models, while those of SAP2, SAP4 and SAP6 were higher in the in vivo model. Furthermore, SAP5 was highly upregulated in the in vivo and RHE models. For SAP9 and SAP10 similar gene expression levels were observed in all model systems. PLB genes were not considerably upregulated in biofilms, while LIP1-3, LIP5-7 and LIP9-10 were highly overexpressed in both in vitro models. Furthermore, an elevated lipase activity was detected in supernatans of biofilms grown in the MTP and RHE model.

Conclusions

Our findings show that HWP1 and most of the genes belonging to the ALS, SAP and LIP gene families are upregulated in C. albicans biofilms. Comparison of the fold expression between the various model systems revealed similar expression levels for some genes, while for others model-dependent expression levels were observed. This suggests that data obtained in one biofilm model cannot be extrapolated to other model systems. Therefore, the need to use multiple model systems when studying the expression of genes encoding potential virulence factors in C. albicans biofilms is highlighted.
Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae
Authors: Wu Fu-Hui , Chan Ming-Tsair , Liao De-Chih , Hsu Chen-Tran , Lee Yi-Wei , Daniell Henry , Duvall Melvin R , Lin Choun-Sea
Published: April 2010

Background

Oncidium spp. produce commercially important orchid cut flowers. However, they are amenable to intergeneric and inter-specific crossing making phylogenetic identification very difficult. Molecular markers derived from the chloroplast genome can provide useful tools for phylogenetic resolution.

Results

The complete chloroplast genome of the economically important Oncidium variety Onc. Gower Ramsey (Accession no. GQ324949) was determined using a polymerase chain reaction (PCR) and Sanger based ABI sequencing. The length of the Oncidium chloroplast genome is 146,484 bp. Genome structure, gene order and orientation are similar to Phalaenopsis, but differ from typical Poaceae, other monocots for which there are several published chloroplast (cp) genome. The Onc. Gower Ramsey chloroplast-encoded NADH dehydrogenase (ndh) genes, except ndhE, lack apparent functions. Deletion and other types of mutations were also found in the ndh genes of 15 other economically important Oncidiinae varieties, except ndhE in some species. The positions of some species in the evolution and taxonomy of Oncidiinae are difficult to identify. To identify the relationships between the 15 Oncidiinae hybrids, eight regions of the Onc. Gower Ramsey chloroplast genome were amplified by PCR for phylogenetic analysis. A total of 7042 bp derived from the eight regions could identify the relationships at the species level, which were supported by high bootstrap values. One particular 1846 bp region, derived from two PCR products (trnH
GUG -psbA and trnF
GAA-ndhJ) was adequate for correct phylogenetic placement of 13 of the 15 varieties (with the exception of Degarmoara Flying High and Odontoglossum Violetta von Holm). Thus the chloroplast genome provides a useful molecular marker for species identifications.

Conclusion

In this report, we used Phalaenopsis. aphrodite as a prototype for primer design to complete the Onc. Gower Ramsey genome sequence. Gene annotation showed that most of the ndh genes inOncidiinae, with the exception of ndhE, are non-functional. This phenomenon was observed in all of the Oncidiinae species tested. The genes and chloroplast DNA regions that would be the most useful for phylogenetic analysis were determined to be the trnH
GUG-psbA and the trnF
GAA-ndhJ regions. We conclude that complete chloroplast genome information is useful for plant phylogenetic and evolutionary studies in Oncidium with applications for breeding and variety identification.
Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats
Authors: Lu Haitao , Zhao Jungong , Li Minghua , Cheng Yingsheng , Li Yongdong , You Xiaofang , Zhao Yuwu
Published: April 2010

Background

The present investigation was designed to elucidate the use of dynamic contrast enhanced perfusion MR imaging (DCE pMRI) in characterizing hyperemia, including microvessel changes, and to examine whether DCE pMRI can predict benign or malignant hyperemia.

Methods

Sprague-Dawley rats underwent middle cerebral artery occlusion (MCAO) by intraluminal suture placement. All rats were randomized to 4 groups: MCAO for 0.5 hours followed by saline treatment (10 ml/kg; group 1); MCAO for 3 hours followed by treatment with saline (group 2) or urokinase (25000 IU/kg; group 3); and MCAO for 6 hours followed by urokinase treatment (group 4). Relative cerebral blood volume (rCBV) and relative maximum slope of increase of the signal intensity time curve (rMSI) were quantitatively analyzed from MRI. Microvessel diameter and blood-brain barrier disruption obtained by laser scanning confocal microscopy (LSCM) as well as transmission electron microscopy (TEM) were obtained for correlative study.

Results

Benign hyperemia was noticed only in group 1; malignant hyperemia was seen in group 3. Although the rCBV of malignant hyperemia was slightly higher than in benign hyperemia (P > 0.05), the rMSI, on the other hand, was significantly lower (P < 0.05). Fluoro-isothiocyanate dextran (FITC-dextran) extravasations, marked glial end-foot process swelling, and significant vasodilatation were seen in malignant hyperemia, while no or mild leakage of FITC-dextran and slight glial end-foot process swelling occurred in benign hyperemia.

Conclusion

Our findings indicate that DCE pMRI can characterize post-ischemic hyperemia and correlates well with microvascular damage.
Randomized controlled trial of artesunate or artemether in Vietnamese adults with severe falciparum malaria
Authors: Phu Nguyen H , Tuan Phung Q , Day Nicholas , Mai Nguyen TH , Chau Tran TH , Chuong Ly V , Sinh Dinh X , White Nicholas J , Farrar Jeremy , Hien Tran T
Published: April 2010

Background

Both artemether and artesunate have been shown to be superior to quinine for the treatment of severe falciparum malaria in Southeast Asian adults, although the magnitude of the superiority has been greater for artesunate than artemether. These two artemisinin derivatives had not been compared in a randomized trial.

Methods

A randomized double blind trial in 370 adults with severe falciparum malaria; 186 received intramuscular artesunate (2.4 mg/kg immediately followed by 1.2 mg/kg at 12 hours then 24 hours then daily) and 184 received intramuscular artemether (3.6 mg per kilogram immediately followed by 1.8 mg per kilogram daily) was conducted in Viet Nam. Both drugs were given for a minimum of 72 hours.

Results

There were 13 deaths in the artesunate group (7 percent) and 24 in the artemether group (13 percent); P = 0.052; relative risk of death in the patients given artesunate, 0.54; (95 percent confidence interval 0.28-1.02). Parasitaemia declined more rapidly in the artesunate group. Both drugs were very well tolerated.

Conclusions

Intramuscular artesunate may be superior to intramuscular artemether for the treatment of severe malaria in adults.
Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma
Authors: Tu Luxia , Liu Zhen , He Xiufang , He Ying , Yang Huiling , Jiang Qingping , Xie Siming , Xiao Guanghui , Li Xin , Yao Kaitai , Fang Weiyi
Published: April 2010

Background

The aim of the present study was to analyze the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including patients' survival time.

Methods

Using real-time PCR, we detected the expression of EIF4G1 in normal nasopharyngeal tissues, immortalized nasopharyngeal epithelial cell lines NP69, NPC tissues and cell lines. EIF4G1 protein expression in NPC tissues was examined using immunohistochemistry. Survival analysis was performed using Kaplan-Meier method. The effect of EIF4G1 on cell invasion and tumorigenesis were investigated.

Results

The expression levels of EIF4G1 mRNA were significantly greater in NPC tissues and cell lines than those in the normal nasopharyngeal tissues and NP69 cells (P < 0.001). Immunohistochemical analysis revealed that the expression of EIF4G1 protein was higher in NPC tissues than that in the nasopharyngeal tissues (P < 0.001). In addition, the levels of EIF4G1 protein in tumors were positively correlated with tumor T classification (P = 0.039), lymph node involvement (N classification, P = 0.008), and the clinical stages (P = 0.003) of NPC patients. Patients with higher EIF4G 1 expression had shorter overall survival time (P = 0.019). Multivariate analysis showed that EIF4G1 expression was an independent prognostic indicator for the overall survival of NPC patients. Using shRNA to knock down the expression of EIF4G1 not only markedly inhibited cell cycle progression, proliferation, migration, invasion, and colony formation, but also dramatically suppressed in vivo xenograft tumor growth.

Conclusion

Our data suggest that EIF4G1 can serve as a biomarker for the prognosis of NPC patients.
HSP90 inhibitor, celastrol, arrests human monocytic leukemia cell U937 at G0/G1 in thiol-containing agents reversible way
Authors: Peng Bin , Xu Limin , Cao Fanfan , Wei Tingxuan , Yang Chunxin , Uzan Georges , Zhang Denghai
Published: April 2010

Background

Because some of heat shock protein 90's (HSP90) clients are key cell cycle regulators, HSP90 inhibition can affect the cell cycle. Recently, celastrol is identified both as a novel inhibitor of HSP90 and as a potential anti-tumor agent. However, this agent's effects on the cell cycle are rarely investigated. In this study, we observed the effects of celastrol on the human monocytic leukemia cell line U937 cell cycle.

Results

Celastrol affected the proliferation of U937 in a dose-dependent way, arresting the cell cycle at G0/G1 with 400 nM doses and triggering cell death with doses above 1000 nM. Cell cycle arrest was accompanied by inhibition of HSP90 ATPase activity and elevation in HSP70 levels (a biochemical hallmark of HSP90 inhibition), a reduction in Cyclin D1, Cdk4 and Cdk6 levels, and a disruption of the HSP90/Cdc37/Cdk4 complex. The observed effects of celastrol on the U937 cell cycle were thiol-related, firstly because the effects could be countered by pre-loading thiol-containing agents and secondly because celastrol and thiol-containing agents could react with each other to form new compounds.

Conclusions

Our results disclose a novel action of celastrol-- causing cell cycle arrest at G0/G1 phase based upon thiol-related HSP90 inhibition. Our work suggests celastrol's potential in tumor and monocyte-related disease management.
HDAC2 attenuates TRAIL-induced apoptosis of pancreatic cancer cells
Authors: Schüler Susanne , Fritsche Petra , Diersch Sandra , Arlt Alexander , Schmid Roland M. , Saur Dieter , Schneider Günter
Published: April 2010

Background

Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant tumors with a dismal prognosis and no effective conservative therapeutic strategies. Although it is demonstrated that histone deacetylases (HDACs), especially the class I HDACs HDAC1, 2 and 3 are highly expressed in this disease, little is known about HDAC isoenzyme specific functions.

Results

Depletion of HDAC2, but not HDAC1, in the pancreatic cancer cell lines MiaPaCa2 and Panc1 resulted in a marked sensitization towards the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Correspondingly, the more class I selective HDAC inhibitor (HDACI) valproic acid (VPA) synergized with TRAIL to induce apoptosis of MiaPaCa2 and Panc1 cells. At the molecular level, an increased expression of the TRAIL receptor 1 (DR5), accelerated processing of caspase 8, pronounced cleavage of the BH3-only protein Bid, and increased effector caspase activation was observed in HDAC2-depleted and TRAIL-treated MiaPaCa2 cells.

Conclusions

Our data characterize a novel HDAC2 function in PDAC cells and point to a strategy to overcome TRAIL resistance of PDAC cells, a prerequisite to succeed with a TRAIL targeted therapy in clinical settings.
Pharmacogenetic interaction between dexamethasone and Cd36-deficient segment of spontaneously hypertensive rat chromosome 4 affects triacylglycerol and cholesterol distribution into lipoprotein fractions
Authors: Krupková Michaela , Šedová Lucie , Liška František , Křenová Drahomíra , Křen Vladimír , Šeda Ondřej
Published: April 2010

Dexamethasone (DEX) is known to induce diabetes and dyslipidemia. We have compared fasting triacylglycerol and cholesterol concentrations across 20 lipoprotein fractions and glucose tolerance in control (standard diet) and DEX-treated 7-month-old males of two rat strains, Brown Norway (BN) and congenic BN.SHR-(Il6-Cd36)/Cub (BN.SHR4). These two inbred strains differ in a defined segment of chromosome 4, originally transferred from the spontaneously hypertensive rat (SHR) including the mutantCd36gene, a known target of DEX. Compared to BN, the standard-diet-fed BN.SHR4 showed higher cholesterol and triacylglycerol concentrations across many lipoprotein fractions, particularly in small VLDL and LDL particles. Total cholesterol was decreased by DEX by more than 21% in BN.SHR4 contrasting with the tendency to increase in BN (strain*DEX interaction p = 0.0017). Similar pattern was observed for triacylglycerol concentrations in LDL. The LDL particle size was significantly reduced by DEX in both strains. Also, while control BN and BN.SHR4 displayed comparable glycaemic profiles during oral glucose tolerance test, we observed a markedly blunted DEX induction of glucose intolerance in BN.SHR4 compared to BN. In summary, we report a pharmacogenetic interaction between limited genomic segment with mutatedCd36gene and dexamethasone-induced glucose intolerance and triacylglycerol and cholesterol redistribution into lipoprotein fractions.
Nutritional correlates and dynamics of diabetes in the Nile rat (Arvicanthis niloticus): a novel model for diet-induced type 2 diabetes and the metabolic syndrome
Authors: Chaabo Fadi , Pronczuk Andrzej , Maslova Ekaterina , Hayes KC
Published: April 2010

Background

The prevalence of Metabolic Syndrome and related chronic diseases, among them non-insulin-dependent (type 2) diabetes mellitus, are on the rise in the United States and throughout the world. Animal models that respond to environmental stressors, such as diet, are useful for investigating the outcome and development of these related diseases.

Objective

Within this context, growth and energy relationships were characterized in the Nile rat, an exotic African rodent, as a potential animal model for diet-induced type 2 diabetes mellitus and Metabolic Syndrome.

Methods

Compiled data from several studies established the relationship between age, body weight gain (including abdominal adiposity), food and water consumption, and blood glucose levels as determinants of diabetes in male and female Nile rats. Glucose Tolerance Testing, insulin, HbA1c, blood pressure measurements and plasma lipids further characterized the diabetes in relation to criteria of the Metabolic Syndrome, while diet modification with high-fat, low-fiber or food restriction attempted to modulate the disease.

Results

The Nile rat fed lab chow demonstrates signs of the Metabolic Syndrome that evolve into diet-induced non-insulin-dependent (type 2) diabetes mellitus characterized by hyperinsulinemia with rising blood glucose (insulin resistance), abdominal adiposity, and impaired glucose clearance that precedes increased food and water intake, as well as elevated HbA1c, marked elevation in plasma triglycerides and cholesterol, microalbuminuria, and hypertension. Males are more prone than females with rapid progression to diabetes depending on the challenge diet. In males diabetes segregated into early-onset and late-onset groups, the former related to more rapid growth and greater growth efficiency for the calories consumed. Interestingly, no correlation was found between blood glucose and body mass index (overall adiposity) in older male Nile rats in long term studies, whereas blood glucose and the perirenal fat pad, as well as liver and kidney weight, were positively related to early-onset diabetes. Rats weaned early (4-5 wks) and challenged with a high-fat Western-type diet developed diabetes faster, and body fat accumulation was more apparent, whereas food restriction curtailed it.

Conclusion

The Nile rat fed typical rodent diets develops hyperinsulinemia that precedes hyperglycemia (insulin resistance) leading to diet-induced type 2 diabetes associated with hypertriglyceridemia, hypercholesterolemia, and hypertension. Dietary modulation affected growth rate (weight gain and central adiposity) to impact disease progression. This rodent model represents a novel system of gene-diet interactions affecting energy utilization that can provide insight into the prevention and treatment of the type 2 diabetes and Metabolic Syndrome.
Carbon black and titanium dioxide nanoparticles elicit distinct apoptotic pathways in bronchial epithelial cells
Authors: Hussain Salik , Thomassen Leen CJ , Ferecatu Ioana , Borot Marie-Caroline , Andreau Karine , Martens Johan A , Fleury Jocelyne , Baeza-Squiban Armelle , Marano Francelyne , Boland Sonja
Published: April 2010

Background

Increasing environmental and occupational exposures to nanoparticles (NPs) warrant deeper insight into the toxicological mechanisms induced by these materials. The present study was designed to characterize the cell death induced by carbon black (CB) and titanium dioxide (TiO2) NPs in bronchial epithelial cells (16HBE14o- cell line and primary cells) and to investigate the implicated molecular pathways.

Results

Detailed time course studies revealed that both CB (13 nm) and TiO2(15 nm) NP exposed cells exhibit typical morphological (decreased cell size, membrane blebbing, peripheral chromatin condensation, apoptotic body formation) and biochemical (caspase activation and DNA fragmentation) features of apoptotic cell death. A decrease in mitochondrial membrane potential, activation of Bax and release of cytochrome c from mitochondria were only observed in case of CB NPs whereas lipid peroxidation, lysosomal membrane destabilization and cathepsin B release were observed during the apoptotic process induced by TiO2 NPs. Furthermore, ROS production was observed after exposure to CB and TiO2 but hydrogen peroxide (H2O2) production was only involved in apoptosis induction by CB NPs.

Conclusions

Both CB and TiO2 NPs induce apoptotic cell death in bronchial epithelial cells. CB NPs induce apoptosis by a ROS dependent mitochondrial pathway whereas TiO2 NPs induce cell death through lysosomal membrane destabilization and lipid peroxidation. Although the final outcome is similar (apoptosis), the molecular pathways activated by NPs differ depending upon the chemical nature of the NPs.
Berlin's medical students' smoking habits, knowledge about smoking and attitudes toward smoking cessation counseling
Authors: Kusma Bianca , Quarcoo David , Vitzthum Karin , Welte Tobias , Mache Stefanie , Meyer-Falcke Andreas , Groneberg David A , Raupach Tobias
Published: April 2010

Background

Diseases associated with smoking are a foremost cause of premature death in the world, both in developed and developing countries. Eliminating smoking can do more to improve health and prolong life than any other measure in the field of preventive medicine. Today's medical students will play a prominent role in future efforts to prevent and control tobacco use.

Methods

A cross-sectional, self-administered, anonymous survey of fifth-year medical students in Berlin, Germany was conducted in November 2007. The study explored the prevalence of smoking among medical students. We assessed their current knowledge regarding tobacco dependence and the effectiveness of smoking cessation methods. Students' perceived competence to counsel smokers and promote smoking cessation treatments was also explored. Analyses were based on responses from 258 students (86.6% response rate).

Results

One quarter of the medical students surveyed were current smokers. The smoking rate was 22.1% among women, 32.4% among men. Students underestimated smoking-related mortality and the negative effect of smoking on longevity. A considerable number of subjects erroneously assumed that nicotine causes coronary artery disease. Students' overall knowledge of the effectiveness of smoking cessation methods was inadequate. Only one third of the students indicated that they felt qualified to counsel patients about tobacco dependence.

Conclusions

This study reveals serious deficiencies in knowledge and counseling skills among medical students in our sample. The curriculum of every medical school should include a tobacco module. Thus, by providing comprehensive training in nicotine dependence interventions to medical students, smokers will have access to the professional expertise they need to quit smoking.
Flux balance analysis accounting for metabolite dilution
Authors: Benyamini Tomer , Folger Ori , Ruppin Eytan , Shlomi Tomer
Published: April 2010

Flux balance analysis is a common method for predicting steady-state flux distributions within metabolic networks, accounting for the growth demand for the synthesis of a predefined set of essential biomass precursors. Ignoring the growth demand for the synthesis of intermediate metabolites required for balancing their dilution leads flux balance analysis to false predictions in some cases. Here, we present metabolite dilution flux balance analysis, which addresses this problem, resulting in improved metabolic phenotype predictions.
This issue of GEOPHYSICS
Authors: Grechka Vladimir
Published: April 2010

In this article, the Editor of Geophysics provides an overview of all technical articles in this issue of the journal.
Intellectual Property
Authors: Walker David A.
Published: April 2010

The Intellectual Property department in each issue of Geophysics provides U.S. patent abstracts, published patent abstracts of U.S. patent applications, and published abstracts filed under the Patent Cooperation Treaty (PCT).