bluuebunny/crossref_metadata_2025 huggingface dataset:
Pasteurization of Food and Beverages by High Pressure Processing (HPP) at Room Temperature: Inactivation of Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Salmonella, and Other Microbial Pathogens
Authors: Silva Filipa Vinagre M. , Evelyn Evelyn
Published: January 2023

Vegetative pathogens actively grow in foods, metabolizing and dividing their cells. They have consequently become a focus of concern for the food industry, food regulators and food control agencies. Although much has been done by the food industry and food regulatory agencies, foodborne outbreaks are still reported globally, causing illnesses, hospitalizations, and in certain cases, deaths, together with product recalls and subsequent economic losses. Major bacterial infections from raw and processed foods are caused by Escherichia coli serotype O157:H7, Salmonella enteritidis, and Listeria monocytogenes. High pressure processing (HPP) (also referred to as high hydrostatic pressure, HHP) is a non-thermal pasteurization technology that relies on very high pressures (400–600 MPa) to inactivate pathogens, instead of heat, thus causing less negative impact in the food nutrients and quality. HPP can be used to preserve foods, instead of chemical food additives. In this study, a review of the effect of HPP treatments on major vegetative bacteria in specific foods was carried out. HPP at 600 MPa, commonly used by the food industry, can achieve the recommended 5–8-log reductions in E. coli, S. enteritidis, L. monocytogenes, and Vibrio. Staphylococcus aureus presented the highest resistance to HPP among the foodborne vegetative pathogens investigated, followed by E. coli. More susceptible L. monocytogenes and Salmonella spp. bacteria were reduced by 6 logs at pressures within 500–600 MPa. Vibrio spp. (e.g., raw oysters), Campylobacter jejuni, Yersinia enterocolitica, Citrobacter freundii and Aeromonas hydrophila generally required lower pressures (300–400 MPa) for inactivation. Bacterial species and strain, as well as the food itself, with a characteristic composition, affect the microbial inactivation. This review demonstrates that HPP is a safe pasteurization technology, which is able to achieve at least 5-log reduction in major food bacterial pathogens, without the application of heat.
Image Processing Approach for Grading IVF Blastocyst: A State-of-the-Art Review and Future Perspective of Deep Learning-Based Models
Authors: Isa Iza Sazanita , Yusof Umi Kalsom , Mohd Zain Murizah
Published: January 2023

The development of intelligence-based methods and application systems has expanded for the use of quality blastocyst selection in in vitro fertilization (IVF). Significant models on assisted reproductive technology (ART) have been discovered, including ones that process morphological image approaches and extract attributes of blastocyst quality. In this study, (1) the state-of-the-art in ART is established using an automated deep learning approach, applications for grading blastocysts in IVF, and related image processing techniques. (2) Thirty final publications in IVF and deep learning were found by an extensive literature search from databases using several relevant sets of keywords based on papers published in full-text English articles between 2012 and 2022. This scoping review sparks fresh thought in deep learning-based automated blastocyst grading. (3) This scoping review introduces a novel notion in the realm of automated blastocyst grading utilizing deep learning applications, showing that these automated methods can frequently match or even outperform skilled embryologists in particular deep learning tasks. This review adds to our understanding of the procedure for selecting embryos that are suitable for implantation and offers important data for the creation of an automated computer-based system for grading blastocysts that applies deep learning.
Acknowledgment to the Reviewers of Applied Biosciences in 2022
Authors:   Applied Biosciences Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Earthly Beasts and Heavenly Creatures: Animal Realms in Early Medieval Chinese Tombs and Cave Temples
Authors: Clydesdale Heather
Published: January 2023

This analysis of the fabricated worlds in tombs and cave temples of China’s Hexi Corridor shows that animals are integral to concepts of earthly and heavenly realms. Changes in animal imagery from the third through sixth centuries connect to the region’s social, cultural, and demographic transformations, including an embrace of pastoralism followed by increasing cosmopolitanism with the spread of Buddhism. A profusion of domestic animals in Wei-Jin tombs establish microcosms, while otherworldly creatures on entrances and coffins play supernatural roles. Western Jin tombs emphasize fantastic beasts over familiar ones and fuel the mysticism of this era. A Sixteen Kingdoms tomb represents the synthesis of the celestial and terrestrial, setting the stage for Buddhist cave temples. In these, real-world animals are all but expunged while imaginary beasts adapt easily to the new habitat. The proliferation of human figures in the form of buddhas and bodhisattvas not only crowd out animals but indicates that the introduction of Buddhism ushers in an anthropocentric view of earthly life and paradise.
On the Strong Starlikeness of the Bernardi Transform
Authors: Orouji Zahra , Ebadian Ali , Cho Nak Eun
Published: January 2023

Many papers concern both the starlikeness and the convexity of Bernardi integral operator. Using the Nunokawa’s Lemma, we want to determine conditions for the strong starlikeness of the Bernardi transform of normalized analytic functions g, such that |arg{g′(z)}| <απ2 in the open unit disk Δ where 0<α<2. Our results include the results of Mocanu, Nunokawa and others on the Libera transform.
On Star Selection Principles Theory
Authors: Kočinac Ljubiša
Published: January 2023

The aim of this paper is to review up-to-date recent results in the field of star selection principles, a rapidly growing area of topology, and to present a few new results.
Arabic Tweets-Based Sentiment Analysis to Investigate the Impact of COVID-19 in KSA: A Deep Learning Approach
Authors: Alqarni Arwa , Rahman Atta
Published: January 2023

The World Health Organization (WHO) declared the outbreak of Coronavirus disease 2019 (COVID-19) a pandemic on 11 March 2020. The evolution of this pandemic has raised global health concerns, making people worry about how to protect themselves and their families. This has greatly impacted people’s sentiments. There was a dire need to investigate a large amount of social data such as tweets and others that emerged during the post-pandemic era for the assessment of people’s sentiments. As a result, this study aims at Arabic tweet-based sentiment analysis considering the COVID-19 pandemic in Saudi Arabia. The datasets have been collected in two different periods in three major regions in Saudi Arabia, which are: Riyadh, Dammam, and Jeddah. Tweets were annotated with three sentiments: positive, negative, and neutral after due pre-processing. Convolutional neural networks (CNN) and bi-directional long short memory (BiLSTM) deep learning algorithms were applied for classifying the sentiment of Arabic tweets. This experiment showed that the performance of CNN achieved 92.80% accuracy. The performance of BiLSTM was scored at 91.99% in terms of accuracy. Moreover, as an outcome of this study, an overwhelming upsurge in negative sentiments were observed in the dataset during COVID-19 compared to the negative sentiments of the dataset before COVID-19. The technique has been compared with the state-of-the-art techniques in the literature and it was observed that the proposed technique is promising in terms of various performance parameters.
AI-Driven Robust Kidney and Renal Mass Segmentation and Classification on 3D CT Images
Authors: Liu Jingya , Yildirim Onur , Akin Oguz , Tian Yingli
Published: January 2023

Early intervention in kidney cancer helps to improve survival rates. Abdominal computed tomography (CT) is often used to diagnose renal masses. In clinical practice, the manual segmentation and quantification of organs and tumors are expensive and time-consuming. Artificial intelligence (AI) has shown a significant advantage in assisting cancer diagnosis. To reduce the workload of manual segmentation and avoid unnecessary biopsies or surgeries, in this paper, we propose a novel end-to-end AI-driven automatic kidney and renal mass diagnosis framework to identify the abnormal areas of the kidney and diagnose the histological subtypes of renal cell carcinoma (RCC). The proposed framework first segments the kidney and renal mass regions by a 3D deep learning architecture (Res-UNet), followed by a dual-path classification network utilizing local and global features for the subtype prediction of the most common RCCs: clear cell, chromophobe, oncocytoma, papillary, and other RCC subtypes. To improve the robustness of the proposed framework on the dataset collected from various institutions, a weakly supervised learning schema is proposed to leverage the domain gap between various vendors via very few CT slice annotations. Our proposed diagnosis system can accurately segment the kidney and renal mass regions and predict tumor subtypes, outperforming existing methods on the KiTs19 dataset. Furthermore, cross-dataset validation results demonstrate the robustness of datasets collected from different institutions trained via the weakly supervised learning schema.
In Vivo Biomechanical Measurements of the Cornea
Authors: Li Fanshu , Wang Kehao , Liu Ziyuan
Published: January 2023

In early corneal examinations, the relationships between the morphological and biomechanical features of the cornea were unclear. Although consistent links have been demonstrated between the two in certain cases, these are not valid in many diseased states. An accurate assessment of the corneal biomechanical properties is essential for understanding the condition of the cornea. Studies on corneal biomechanics in vivo suggest that clinical problems such as refractive surgery and ectatic corneal disease are closely related to changes in biomechanical parameters. Current techniques are available to assess the mechanical characteristics of the cornea in vivo. Accordingly, various attempts have been expended to obtain the relevant mechanical parameters from different perspectives, using the air-puff method, ultrasound, optical techniques, and finite element analyses. However, a measurement technique that can comprehensively reflect the full mechanical characteristics of the cornea (gold standard) has not yet been developed. We review herein the in vivo measurement techniques used to assess corneal biomechanics, and discuss their advantages and limitations to provide a comprehensive introduction to the current state of technical development to support more accurate clinical decisions.
Bcl-xL Promotes the Survival of Motor Neurons Derived from Neural Stem Cells
Authors: Wu Yunqin , Peng Xiaohua , Ang Song , Gao Yue , Chi Yue , Wang Jinling , Tang Chengcheng , Zhou Xiaoqing , Feng Yanxian , Zhang Kun , Zou Qingjian , Chen Min
Published: January 2023

Neural stem cell (NSC) transplantation creates new hope for the treatment of neurodegenerative disorders by direct differentiation into neurons. However, this technique is limited by poor survival and functional neuron deficiency. In this research study, we generated pro-survival murine NSCs (mNSCs) via the ectopic expression of Bcl-xL. A doxycycline (Dox)-inducible Ngn2-Isl1-Lhx3 system was also integrated into the mNSC genome. The four gene-modified mNSCs can rapidly and effectively differentiate into motor neurons after Dox treatments. Ectopic Bcl-xL could resist replating-induced stress, glutamate toxicity, neuronal apoptosis and remarkably promote the survival of motor neurons. Taken together, we established genetically modified mNSCs with improved survival, which may be useful for motor neuron degenerative diseases.
Oxidative Stress, Environmental Pollution, and Lifestyle as Determinants of Asthma in Children
Authors: Vincenzo Serena Di , Ferrante Giuliana , Ferraro Maria , Cascio Caterina , Malizia Velia , Licari Amelia , La Grutta Stefania , Pace Elisabetta
Published: January 2023

Exposure to cigarette smoke, allergens, viruses, and other environmental contaminants, as well as a detrimental lifestyle, are the main factors supporting elevated levels of airway oxidative stress. Elevated oxidative stress results from an imbalance in reactive oxygen species (ROS) production and efficiency in antioxidant defense systems. Uncontrolled increased oxidative stress amplifies inflammatory processes and tissue damage and alters innate and adaptive immunity, thus compromising airway homeostasis. Oxidative stress events reduce responsiveness to corticosteroids. These events can increase risk of asthma into adolescence and prompt evolution of asthma toward its most severe forms. Development of new therapies aimed to restore oxidant/antioxidant balance and active interventions aimed to improve physical activity and quality/quantity of food are all necessary strategies to prevent asthma onset and avoid in asthmatics evolution toward severe forms of the disease.
Neuromuscular and Biomechanical Jumping and Landing Deficits in Young Female Handball Players
Authors: Cadens Maria , Planas-Anzano Antoni , Peirau-Terés Xavier , Benet-Vigo Ariadna , Fort-Vanmeerhaeghe Azahara
Published: January 2023

Neuromuscular and biomechanical imbalances that exist in jumping and landing actions should be examined in order to intervene to decrease the risk of ACL injury. The main aim of this study was to analyse and compare, by chronological age, jumping and landing deficits in young female handball players using the Tuck Jump Assessment (TJA). A secondary aim was to relate the qualitative asymmetry values detected using the TJA to the quantitative asymmetry values detected starting from the single leg countermovement jump (SL-CMJ). Sixty-one young female handball players (age: 14.3 ± 1.5 years) were distributed into three groups: U12, U14 and U16 and performed the TJA test and the single leg countermovement jump (SL-CMJ). The female U12 category players obtained the highest scores in the TJA and there were significant differences between the U12 (12.11 ± 1.97) and U14 (10.89 ± 1.74) categories (p = 0.017; ES = 0.374). In the U12 category, the female players presented larger interlimb asymmetry magnitudes in the SL-CMJ test; they also obtained higher scores in the qualitative criteria of the TJA test that referred to asymmetry (r = 0.43; p = 0.027). The analysis of the jumping and landing pattern using TJA allowed us to identify that the lower extremity valgus at landing, foot contact timing not equal and landing contact noise are the main biomechanical deficits in young female handball players. Furthermore, the asymmetry values assessed qualitatively (TJA) are associated with the asymmetry values assessed quantitatively (difference in jump achieved with each limb in the SL-CMJ test) in younger categories.
Acknowledgment to the Reviewers of Biology in 2022
Authors:   Biology Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
Comparative Transcriptome Analysis of Pueraria lobata Provides Candidate Genes Involved in Puerarin Biosynthesis and Its Regulation
Authors: Xi Huiting , Zhu Yaru , Sun Wenwen , Tang Nan , Xu Zhengqin , Shang Xiaohong , Zhang Yansheng , Yan Huabing , Li Changfu
Published: January 2023

Pueraria lobata is a traditional Chinese herb in which an isoflavone C-glucoside, namely puerarin, has received the utmost interest due to its medicinal properties. To date, the biogenesis of puerarin, especially its C-glucosyl reaction in the pathway, remains poorly understood. Moreover, the transcription factors (TFs) that regulate puerarin biosynthesis in P. lobata have not been reported. Here, we performed phytochemical studies on the different developmental stages of the root, stem, and leaf tissues of two P. lobata cultivars, which suggested that both the roots and stems of P. lobata were the sites of puerarin biosynthesis. RNA-sequencing was conducted with the root and stem tissues of the two cultivars under different stages, and the clean reads were mapped to the recently published genome of P. lobata var. thomsonii, yielding the transcriptome dataset. A detailed analysis of the gene expression data, gene coexpression network, and phylogeny proposed several C-GTs that likely participate in puerarin biosynthesis. The first genome-wide analysis of the whole MYB superfamily in P. lobata presented here identified a total of 123 nonredundant PlMYB genes that were significantly expressed in the analyzed tissues. The phylogenetic analysis of PlMYBs with other plant MYB proteins revealed strong PlMYB candidates that may regulate the biosynthesis of isoflavones, such as puerarin.
Glycosylated Lipopeptides—Synthesis and Evaluation of Antimicrobial Activity and Cytotoxicity
Authors: Sikora Karol , Bauer Marta , Bartoszewska Sylwia , Neubauer Damian , Kamysz Wojciech
Published: January 2023

Ultrashort cationic lipopeptides (USCLs) are promising antimicrobial agents that may be used to combat pathogens such as bacteria and fungi. USCLs consist of a few basic amino acid residues and at least one lipid moiety, usually a fatty acid chain. Generally, USCLs are potent antimicrobials but their major shortcoming is a relatively high cytotoxicity and hemolytic activity. Glycopeptide antibiotics (e.g. vancomycin) are essential in combating bacterial infections and are popular in medicinal practice. However, literature concerning the effect of glycosylation of peptides on their antimicrobial activity is rather scarce. For the first time, this study highlights the effect of USCLs glycosylation on in vitro biological activity. The aim of this study was to evaluate the impact of glycosylation of a series of USCLs on antimicrobial activity, cytotoxicity and hemolytic activity. Straight-chain fatty acids (C14, C16, C18) were attached to the N-terminal amino group of tripeptides—SRR-NH2, RSR-NH2 and RRS-NH2. Two groups of the lipopeptides were synthetized, the first with unmodified L-serine (USCLs) and the other with L-serine O-glycosylated by N-acetyl-β-d-glucosamine to produce new class of glycosylated ultrashort cationic lipopeptide (gUSCLs). Both USCLs and gUSCLs were tested against planktonic and biofilm cultures of ESKAPE strains (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp.) and Candida glabrata, and hemolytic activity on human erythrocytes and cytotoxicity against the HaCaT cell line was examined. Generally, USCLs and gUSCLs proved to be active against all the tested strains. The highest activity displayed was by lipopeptides containing the C18 fatty acid. Antimicrobial, hemolytic and cytotoxic activities were mainly correlated with amino acid sequence (position of serine/glycosylated serine) and hydrophobicity of molecule and were found to be highly strain-dependent. In general, glycosylation did not guarantee an increased antimicrobial activity or a decreased hemolytic and cytotoxic activities. However, in some cases, gUSCLs proved to be superior to their USCLs analogs. The most pronounced differences were found for peptides with C18 fatty acid and serine at the first and second position against both planktonic cells and biofilm of C. glabrata, as well as the second and third position against S. aureus. It is noteworthy that gUSCLs were also more active against biofilm than were USCLs.
Laboratory and Metabolomic Fingerprint in Heart Failure with Preserved Ejection Fraction: From Clinical Classification to Biomarker Signature
Authors: Palazzuoli Alberto , Tramonte Francesco , Beltrami Matteo
Published: January 2023

Heart failure with preserved ejection fraction (HFpEF) remains a poorly characterized syndrome with many unknown aspects related to different patient profiles, various associated risk factors and a wide range of aetiologies. It comprises several pathophysiological pathways, such as endothelial dysfunction, myocardial fibrosis, extracellular matrix deposition and intense inflammatory system activation. Until now, HFpEF has only been described with regard to clinical features and its most commonly associated risk factors, disregarding all biological mechanisms responsible for cardiovascular deteriorations. Recently, innovations in laboratory and metabolomic findings have shown that HFpEF appears to be strictly related to specific cells and molecular mechanisms’ dysregulation. Indeed, some biomarkers are efficient in early identification of these processes, adding new insights into diagnosis and risk stratification. Moreover, recent advances in intermediate metabolites provide relevant information on intrinsic cellular and energetic substrate alterations. Therefore, a systematic combination of clinical imaging and laboratory findings may lead to a ‘precision medicine’ approach providing prognostic and therapeutic advantages. The current review reports traditional and emerging biomarkers in HFpEF and it purposes a new diagnostic approach based on integrative information achieved from risk factor burden, hemodynamic dysfunction and biomarkers’ signature partnership.
A Tribute to Phil Bourne—Scientist and Human
Authors: Mura Cameron , Candelier Emma , Xie Lei
Published: January 2023

This Special Issue of Biomolecules[...]
Ragweed Major Allergen Amb a 11 Recombinant Production and Clinical Implications
Authors: Tamaș Tudor-Paul , Buzan Maria-Roxana , Zbîrcea Lauriana-Eunice , Cotarcă Monica-Daniela , Grijincu Manuela , Păunescu Virgil , Panaitescu Carmen , Chen Kuan-Wei
Published: January 2023

Ragweed pollen is highly allergenic and elicits type I hypersensitivity reactions in the exposed populations. Amb a 11 is a recently discovered component of this pollen, and its biological role in allergy is still being researched. In our study, ragweed allergy patients were recruited prospectively over a three-year period; a comprehensive questionnaire was administered, and sera were collected and stored. The production of recombinant Amb a 11 was achieved in parallel with patients’ recruitment. The gene coding for mature protein was inserted in E. coli and in Sf9 Spodoptera frugiperda cells. The recombinant allergens (designated eAmb a 11 and iAmb a 11) were tested for His-tag presence in Western blot. IgE reactivity was evaluated in 150 patients’ sera for both recombinant allergen forms in ELISA, with 5 positive sera being tested further by hRBL (humanized rat basophilic leukemia) hexosaminidase release assay. Both allergen forms were proven to be IgE-reactive His-tagged proteins, with an extensive overlap of positive sera (92 toward the former recombinant allergen, 100 toward the latter) and an overall Amb a 11 sensitization prevalence estimated at 68.67%. The hRBL mediator release assay revealed a significant, slightly weaker effect of recombinant allergens when compared with nAmb a 1. Sensitization to this major allergen appears to be associated with more severe asthma symptoms (OR = 4.71, 95% CI = 1.81–12.21). In conclusion, recombinant Amb a 11 is a bona fide allergen, which is IgE-reactive and an inducer of hRBL degranulation. It is an important IgE-reactive component from ragweed pollen, with high IgE sensitization prevalence in the sample population and allergenicity of the recombinant allergen comparable to Amb a 1.
Inflammatory Cytokine-Induced HIF-1 Activation Promotes Epithelial–Mesenchymal Transition in Endometrial Epithelial Cells
Authors: Hashimoto Yoshiko , Tsuzuki-Nakao Tomoko , Kida Naoko , Matsuo Yoshiyuki , Maruyama Tetsuo , Okada Hidetaka , Hirota Kiichi
Published: January 2023

The endometrium undergoes repeated proliferation and shedding during the menstrual cycle. Significant changes to this environment include fluctuations in the partial pressure of oxygen, exposure to a high-cytokine environment associated with intrauterine infection, and inflammation. Chronic endometritis is a condition wherein mild inflammation persists in the endometrium and is one of the causes of implantation failure and miscarriage in early pregnancy. It is thought that the invasion of embryos into the endometrium requires epithelial–mesenchymal transition (EMT)-associated changes in the endometrial epithelium. However, the effects of inflammation on the endometrium remain poorly understood. In this study, we investigated the effects of the intrauterine oxygen environment, hypoxia-inducible factor (HIF), and inflammation on the differentiation and function of endometrial epithelial cells. We elucidated the ways in which inflammatory cytokines affect HIF activity and EMT in an immortalized cell line (EM-E6/E7/TERT) derived from endometrial epithelium. Pro-inflammatory cytokines caused significant accumulation of HIF-1α protein, increased HIF-1α mRNA levels, and enhanced hypoxia-induced accumulation of HIF-1α protein. The combined effect of inflammatory cytokines and hypoxia increased the expression of EMT-inducing factors and upregulated cell migration. Our findings indicate that pro-inflammatory factors, including cytokines and LPS, work synergistically with hypoxia to activate HIF-1 and promote EMT in endometrial epithelial cells.
TGF-Beta Modulates the Integrity of the Blood Brain Barrier In Vitro, and Is Associated with Metabolic Alterations in Pericytes
Authors: Schumacher Leonie , Slimani Rédouane , Zizmare Laimdota , Ehlers Jakob , Kleine Borgmann Felix , Fitzgerald Julia C. , Fallier-Becker Petra , Beckmann Anja , Grißmer Alexander , Meier Carola , El-Ayoubi Ali , Devraj Kavi , Mittelbronn Michel , Trautwein Christoph , Naumann Ulrike
Published: January 2023

The blood–brain barrier (BBB) is a selectively permeable boundary that separates the circulating blood from the extracellular fluid of the brain and is an essential component for brain homeostasis. In glioblastoma (GBM), the BBB of peritumoral vessels is often disrupted. Pericytes, being important to maintaining BBB integrity, can be functionally modified by GBM cells which induce proliferation and cell motility via the TGF-β-mediated induction of central epithelial to mesenchymal transition (EMT) factors. We demonstrate that pericytes strengthen the integrity of the BBB in primary endothelial cell/pericyte co-cultures as an in vitro BBB model, using TEER measurement of the barrier integrity. In contrast, this effect was abrogated by TGF-β or conditioned medium from TGF-β secreting GBM cells, leading to the disruption of a so far intact and tight BBB. TGF-β notably changed the metabolic behavior of pericytes, by shutting down the TCA cycle, driving energy generation from oxidative phosphorylation towards glycolysis, and by modulating pathways that are necessary for the biosynthesis of molecules used for proliferation and cell division. Combined metabolomic and transcriptomic analyses further underscored that the observed functional and metabolic changes of TGF-β-treated pericytes are closely connected with their role as important supporting cells during angiogenic processes.
An Overview of Potential Natural Photosensitizers in Cancer Photodynamic Therapy
Authors: Aziz Bushra , Aziz Iffat , Khurshid Ahmat , Raoufi Ehsan , Esfahani Fahime Nasr , Jalilian Zahra , Mozafari M. R. , Taghavi Elham , Ikram Masroor
Published: January 2023

Cancer is one of the main causes of death worldwide. There are several different types of cancer recognized thus far, which can be treated by different approaches including surgery, radiotherapy, chemotherapy or a combination thereof. However, these approaches have certain drawbacks and limitations. Photodynamic therapy (PDT) is regarded as an alternative noninvasive approach for cancer treatment based on the generation of toxic oxygen (known as reactive oxygen species (ROS)) at the treatment site. PDT requires photoactivation by a photosensitizer (PS) at a specific wavelength (λ) of light in the vicinity of molecular oxygen (singlet oxygen). The cell death mechanisms adopted in PDT upon PS photoactivation are necrosis, apoptosis and stimulation of the immune system. Over the past few decades, the use of natural compounds as a photoactive agent for the selective eradication of neoplastic lesions has attracted researchers’ attention. Many reviews have focused on the PS cell death mode of action and photonanomedicine approaches for PDT, while limited attention has been paid to the photoactivation of phytocompounds. Photoactivation is ever-present in nature and also found in natural plant compounds. The availability of various laser light setups can play a vital role in the discovery of photoactive phytocompounds that can be used as a natural PS. Exploring phytocompounds for their photoactive properties could reveal novel natural compounds that can be used as a PS in future pharmaceutical research. In this review, we highlight the current research regarding several photoactive phytocompound classes (furanocoumarins, alkaloids, poly-acetylenes and thiophenes, curcumins, flavonoids, anthraquinones, and natural extracts) and their photoactive potential to encourage researchers to focus on studies of natural agents and their use as a potent PS to enhance the efficiency of PDT.
Acknowledgment to the Reviewers of Biomimetics in 2022
Authors:   Biomimetics Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
An Underactuated Adaptive Microspines Gripper for Rough Wall
Authors: Li Xinxin , Chen Wenqing , Li Xiaosong , Hou Xin , Zhao Qian , Meng Yonggang , Tian Yu
Published: January 2023

Wall attachment has great potential in a broad range of applications such as robotic grasping, transfer printing, and asteroid sampling. Herein, a new type of underactuated bionic microspines gripper is proposed to attach to an irregular, rough wall. Experimental results revealed that the gripper, profiting from its flexible structure and underactuated linkage mechanism, is capable of adapting submillimeter scale roughness to centimeter scale geometry irregularity in both normal and tangential attachment. The rigid-flexible coupling simulation analysis validated that the rough adaptation was achieved by the passive deformation of the zigzag flexible structure, while the centimeter-scale irregularity adaptation come from the underactuated design. The attachment test of a spine confirmed that a 5 mm sliding distance of the spine tip on the fine brick wall promises a saturated tangential attachment force, which can guide the stiffness design of flexible structure and parameter selection of underactuated linkage. Furthermore, the developed microspines gripper was successfully demonstrated to grasp irregular rocks, tree trunks, and granite plates. This work presents a generally applicable and dexterous passive adaption design to achieve rough wall attachment for flat and curved objects, which promotes the understanding and application of wall attachment.
Non-Destructive and Non-Invasive Measurement of Ethanol and Toxic Alcohol Strengths in Beverages and Spirits Using Portable Raman Spectroscopy
Authors: Papaspyridakou Panagiota , Giannoutsou Panagiota , Orkoula Malvina G.
Published: January 2023

The measurement of ethanol and toxic alcohol (methanol and isopropanol) strengths in beverages and spirits is crucial for health reasons but also for the identification of adulterated products. Many methodologies have been reported in the literature, based mainly on chromatographic and on spectroscopic techniques. Chromatographic techniques are laborious and time-consuming, while spectroscopic techniques are rapid and need no special sample pretreatment. All techniques were only applied to off-line or at-line manner. In the present work, Raman spectroscopy was used for fast and non-destructive measurements. A “through the container” method was developed for a non-invasive analysis, i.e., analysis without unsealing the bottles. This method, coupled with a miniature portable Raman, can serve for in-line measurements in a production line. The optimum laser focus for maximum spirit signal and minimum glass-wall signal was investigated. Calibration curves for the alcohols of interest were constructed and validated. The limits of detections were calculated and proved to be lower than the legitimate values. The influences of the liquor color and the bottle color, shape, and thickness were checked. Twenty-eight alcoholic products were studied. The concentrations found were compared against the nominal values (from the bottle labels).
Semiconducting Polymer Dots for Point-of-Care Biosensing and In Vivo Bioimaging: A Concise Review
Authors: Deng Sile , Li Lingfeng , Zhang Jiaxi , Wang Yongjun , Huang Zhongchao , Chen Haobin
Published: January 2023

In recent years, semiconducting polymer dots (Pdots) have attracted much attention due to their excellent photophysical properties and applicability, such as large absorption cross section, high brightness, tunable fluorescence emission, excellent photostability, good biocompatibility, facile modification and regulation. Therefore, Pdots have been widely used in various types of sensing and imaging in biological medicine. More importantly, the recent development of Pdots for point-of-care biosensing and in vivo imaging has emerged as a promising class of optical diagnostic technologies for clinical applications. In this review, we briefly outline strategies for the preparation and modification of Pdots and summarize the recent progress in the development of Pdots-based optical probes for analytical detection and biomedical imaging. Finally, challenges and future developments of Pdots for biomedical applications are given.
Review of Bacterial Nanocellulose-Based Electrochemical Biosensors: Functionalization, Challenges, and Future Perspectives
Authors: de Assis Samuel Chagas , Morgado Daniella Lury , Scheidt Desiree Tamara , de Souza Samara Silva , Cavallari Marco Roberto , Ando Junior Oswaldo Hideo , Carrilho Emanuel
Published: January 2023

Electrochemical biosensing devices are known for their simple operational procedures, low fabrication cost, and suitable real-time detection. Despite these advantages, they have shown some limitations in the immobilization of biochemicals. The development of alternative materials to overcome these drawbacks has attracted significant attention. Nanocellulose-based materials have revealed valuable features due to their capacity for the immobilization of biomolecules, structural flexibility, and biocompatibility. Bacterial nanocellulose (BNC) has gained a promising role as an alternative to antifouling surfaces. To widen its applicability as a biosensing device, BNC may form part of the supports for the immobilization of specific materials. The possibilities of modification methods and in situ and ex situ functionalization enable new BNC properties. With the new insights into nanoscale studies, we expect that many biosensors currently based on plastic, glass, or paper platforms will rely on renewable platforms, especially BNC ones. Moreover, substrates based on BNC seem to have paved the way for the development of sensing platforms with minimally invasive approaches, such as wearable devices, due to their mechanical flexibility and biocompatibility.
A Study on the Optimization of Atrium Daylight and Energy Performance through Skylight and Shading Design in Commercial Buildings in Cold Zones
Authors: Xue Yibing , Liu Wenhan
Published: January 2023

Atriums play an irreplaceable role within the plan of modern commercial buildings. However, the presence of skylights makes the atrium suffer from unfavorable conditions such as overheating, high energy consumption, and glare, which can be partially reduced by the sun-shading design of skylights. In this paper, we conducted a field test and questionnaire survey on the daylight quality of commercial buildings in cold zones in China to find out the current problems of daylight quality in atriums and to establish a model of commercial atrium. After the dynamic daylight and energy consumption simulation, the sensitivity analysis of daylight and energy performance is performed for the skylight and shading (SAS) design parameters under three shading types. Finally, the Pareto front solution set of optimized commercial atrium daylight, visual comfort, and energy performance for the three shading types has been obtained using a multi-objective search tool. The results show that SRR has the greatest effect on sDA, DGP, and EUI in all shading cases; while SGT has a greater effect on EUI than on daylight quality; FC has a greater effect on EUI than FV on EUI; and LS and LI have a more significant influence on daylight and visual comfort. The optimal SAS values of no shading, fabric shading and louvered shading were screened as SRR is 0.4–0.5, SGT is Double silver Low-E insulating glass; SRR is 0.5–0.6, SGT is Double silver Low-E insulating glass, FV is 0.5–0.7, FC is 0.5–0.6; SRR is 0.6–0.7, SGT is Double silver Low-E insulating glass, LS is 100 mm/125 mm, and LI is 60–70°. It is expected that this study will provide some information and reference for the design of commercial atriums in cold zones in the future.
State-of-the-Art Review of Oxidative Dehydrogenation of Ethane to Ethylene over MoVNbTeOx Catalysts
Authors: Chen Yuxin , Yan Binhang , Cheng Yi
Published: January 2023

Ethylene is mainly produced by steam cracking of naphtha or light alkanes in the current petrochemical industry. However, the high-temperature operation results in high energy demands, high cost of gas separation, and huge CO2 emissions. With the growth of the verified shale gas reserves, oxidative dehydrogenation of ethane (ODHE) becomes a promising process to convert ethane from underutilized shale gas reserves to ethylene at a moderate reaction temperature. Among the catalysts for ODHE, MoVNbTeOx mixed oxide has exhibited superior catalytic performance in terms of ethane conversion, ethylene selectivity, and/or yield. Accordingly, the process design is compact, and the economic evaluation is more favorable in comparison to the mature steam cracking processes. This paper aims to provide a state-of-the-art review on the application of MoVNbTeOx catalysts in the ODHE process, involving the origin of MoVNbTeOx, (post-) treatment of the catalyst, material characterization, reaction mechanism, and evaluation as well as the reactor design, providing a comprehensive overview of M1 MoVNbTeOx catalysts for the oxidative dehydrogenation of ethane, thus contributing to the understanding and development of the ODHE process based on MoVNbTeOx catalysts.
Stability and Requirement for Thiamin in a Cell Culture Feed Used to Produce New Biological Entities
Authors: Schnellbächer Alisa , Zimmer Aline
Published: January 2023

Thiamin is susceptible to heat and oxidation, which is a concern for the development of concentrated and room temperature stable feeds used to produce recombinant proteins. Hence, it is critical to understand the reactivity and necessity of the vitamin in liquid feeds to be able to either develop mitigation strategies to stabilize the vitamin or to remove thiamin from formulations if it is unnecessary. LC-MS/MS was used to investigate thiamin stability in different liquid feed formulations and to identify thiamin degradation products. Results indicate oxidation of thiamin and interaction with amino acids, keto acids, and sulfur containing components. Thiamin necessity in feed was assessed during a fed batch experiment, focusing on cell performance and critical quality attributes of the produced recombinant proteins. The impact of thiamin depletion in the feed on the intra- and extracellular metabolome was investigated using untargeted LC-MS/MS. Results indicate that thiamin can be removed from the feed without affecting the performance or the intra- and extracellular metabolome of the tested cell lines. Overall, profound insights on thiamin reactivity and necessity are presented in this study, suggesting the removal of the dispensable and instable vitamin as a simple means for the development of next generation feeds used to produce therapeutic biological entities.
Total Synthesis of Floyocidin B: 4,5-Regioselective Functionalization of 2-Chloropyridines
Authors: Kleiner Yolanda , Bauer Armin , Hammann Peter , Schuler Sören M. M. , Pöverlein Christoph
Published: January 2023

The recently discovered natural product (NP) (+)-floyocidin B with antimicrobial activity against Mycobacterium tuberculosis displays a hitherto unknown dihydroisoquinolinone scaffold in the class of the epoxyquinone NPs. The 4,5-regioselective functionalization of 2-chloropyridines was identified as a suitable strategy leading to the total syntheses of (+)-floyocidin B and analogs. In this paper, we present the long and winding evolution process to the final synthetic pathway, including model systems for route scouting and elucidation of side products, which enabled us to understand the unique reactivity of this unprecedented scaffold. A special focus was laid on method studies with different 2-chloropyridines, disclosing an unexpected effect of the 2-chloro substituent on the regioselectivity compared to 2-unsubstituted or carbon-substituted pyridines. Finally, a head-to-head comparison with the previously described synthesis of all four stereoisomers of the NP (−)-avicennone C revealed significant differences in the reactivity of these structurally closely related scaffolds.
Gentle Touch and Sucrose for Pain Relief during Suctioning in Preterm Newborns—A Randomized Clinical Trial
Authors: Oliveira Nayara Rodrigues Gomes de , Formiga Cibelle Kayenne Martins Roberto , Ramos Bruna Abreu , Santos Rafaela Noleto dos , Moreira Nayara Nubia de Sousa , Marçal Patricia Gonçalves Evangelista , Amaral Waldemar Naves do
Published: January 2023

Pain management is challenging in neonatal care. We aimed to compare the effects of gentle touch and sucrose on pain relief during suctioning in premature newborns (PTNB). This crossover randomized clinical trial enrolled PTNBs with low birth weight, hemodynamically stable, and requiring suctioning during hospitalization in the neonatal intensive care unit. PTNBs underwent three different suctioning procedures. The first was performed without intervention (baseline). Right after, PTNBs were randomly allocated (sucrose and gentle touch or vice versa) to the next two suctioning procedures. Two validated scales assessed pain: the Neonatal Infant Pain Scale (NIPS) and the Premature Infant Pain Profile-Revised (PIPP-R). We evaluated 50 PTNBs (mean of 28 weeks) with a mean low birth weight of 1050 g; most were under continuous positive airway pressure 37 (74%) and mechanical ventilation 41 (82%). Gentle touch was efficacious for pain relief since NIPS (p = 0.010) compared to baseline. Sucrose was also effective in reducing pain NIPS and PIPP-R (p < 0.001). Although the two interventions reduced pain, no difference was observed between gentle touch and sucrose.
Allogeneic Mesenchymal Stromal Cells as a Global Pediatric Prospective Approach in the Treatment of Respiratory Failure Associated with Surfactant Protein C Dysfunction
Authors: Pelizzo Gloria , Avanzini Maria Antonietta , Croce Stefania , Mandelli Anna , Lenta Elisa , Farolfi Andrea , Valsecchi Chiara , Zirpoli Salvatore , Lanfranchi Giulia , Durante Eleonora , Zoia Elena , Zuccotti Gianvincenzo , Calcaterra Valeria
Published: January 2023

Mesenchymal stromal cells (MSCs) have been proposed as a new therapeutic strategy to treat congenital and acquired respiratory system diseases. We describe a case report of an 18-month-old male patient with progressive chronic respiratory failure, associated with mutations of the surfactant protein C gene (SFTPC) due to c.289G > T variant p.Gly97Ser (rs927644577) and c.176A > G variant (p.His59Arg), submitted to repeated intravenous infusions of allogeneic bone marrow (BM) MSCs. The clinical condition of the patient was monitored. Immunologic studies before and during MSC treatment were performed. No adverse events related to the MSC infusions were recorded. Throughout the MSC treatment period, the patient showed a growth recovery. Starting the second infusion, the patient experienced an improvement in his respiratory condition, with progressive adaptation to mechanical ventilation. After the third infusion, five hours/die of spontaneous breathing was shown, and after infusion IV, spontaneous ventilation for 24/24 h was recorded. A gradual decrease of lymphocytes and cell subpopulations was observed. No variations in the in vitro T cell response to PHA were determined by MSC treatment as well as for the in vitro B cell response. A decrease in IFN-γ, TNF-α, and IL-10 levels was also detected. Even though we cannot exclude an improvement of pulmonary function due to the physiological maturation, the well-known action of MSCs in the repair of lung tissue, together with the sequence of events observed in our patient, may support the therapeutic role of MSCs in this clinical condition. However, further investigations are necessary to confirm the result and long-term follow-up will be mandatory to confirm the benefits on the pulmonary condition.
Parent Perspectives of Ear Health and the Relationship with Children’s Speech and Language in the Longitudinal Study of Indigenous Children
Authors: Morrow Anita , Orr Neil , Nash Kai , Coates Harvey , Cross Cara , Evans John Robert , Gunasekera Hasantha , Harkus Samantha , Harrison Linda , McLeod Sharynne , McMahon Catherine , Neal Katie , Salins Andrea , Macniven Rona
Published: January 2023

Health and well-being are holistic concepts that are perceived to be inseparable for Aboriginal and Torres Strait Islander peoples. We examined relationships between parent-reported ear symptoms for 787 Indigenous children at two time points (age 2–3 years, age 4–5 years) and two parent-reported speech and language outcomes one year later (age 5–6 years). Most parents (80.2%) reported no concern about their child’s expressive language and (93.8%) receptive language. Binary logistic regression models examined ear health as a predictor of children’s expressive and receptive speech and language adjusting for sociodemographic and health covariates. For children without parent-reported ear symptoms, there were lower odds of parental concern about expressive speech and language (aOR = 0.45; 95% CI 0.21–0.99) and receptive language (aOR = 0.24; 95% CI 0.09–0.62). Parents were less likely to have concerns about the child’s expressive speech and language if their child was female, lived in urban or regional areas, had excellent or very good global health, or had no disability when aged 2–5 years. Since parent-reported ear health and speech and language concerns were related, Aboriginal and Torres Strait Islander children could benefit from culturally safe, strength-based, and family-centered integrated speech, language, and ear health services.