bluuebunny/crossref_metadata_2025 huggingface dataset:
Kinetic Study on Heterotrophic Growth of Acetobacterium woodii on Lignocellulosic Substrates for Acetic Acid Production
Authors: Karekar Supriya C. , Srinivas Keerthi , Ahring Birgitte K.
Published: February 2019

Extensive research has been done on examining the autotrophic growth of Acetobacterium woodii with gaseous substrates (hydrogen and carbon dioxide) to produce acetic acid. However, only limited work has been performed on the heterotrophic growth of A. woodii using pure sugars or lignocellulosic feedstocks-derived sugars as substrates. In this study, we examine the growth kinetics and acetic acid production of A. woodii on glucose and xylose. While good growth was observed with glucose as substrate, no significant growth was obtained on xylose. Kinetic studies were performed in batch culture using different concentrations of glucose, ranging from 5 g/L to 40 g/L. The highest acetate production of 6.919 g/L with a product yield of 0.76 g acetic acid/g glucose was observed with 10 g/L glucose as initial substrate concentration. When testing A. woodii on corn stover hydrolysate (CSH) and wheat straw hydrolysate (WSH) formed after pretreatment and enzymatic hydrolysis, we found that A. woodii showed acetic acid production of 7.64 g/L and a product yield of 0.70 g acetic acid/g of glucose on WSH, while the acetic acid production was 7.83 g/L with a product yield of 0.65 g acetic acid/g of glucose on CSH. These results clearly demonstrate that A. woodii performed similarly on pure substrates and hydrolysates, and that the processes were not inhibited by the heterogenous components present in the lignocellulosic feedstock hydrolysates.
Fog vs. Cloud Computing: Should I Stay or Should I Go?
Authors: Pisani Flávia , Martins do Rosario Vanderson , Borin Edson
Published: February 2019

In this article, we work toward the answer to the question “is it worth processing a data stream on the device that collected it or should we send it somewhere else?”. As it is often the case in computer science, the response is “it depends”. To find out the cases where it is more profitable to stay in the device (which is part of the fog) or to go to a different one (for example, a device in the cloud), we propose two models that intend to help the user evaluate the cost of performing a certain computation on the fog or sending all the data to be handled by the cloud. In our generic mathematical model, the user can define a cost type (e.g., number of instructions, execution time, energy consumption) and plug in values to analyze test cases. As filters have a very important role in the future of the Internet of Things and can be implemented as lightweight programs capable of running on resource-constrained devices, this kind of procedure is the main focus of our study. Furthermore, our visual model guides the user in their decision by aiding the visualization of the proposed linear equations and their slope, which allows them to find if either fog or cloud computing is more profitable for their specific scenario. We validated our models by analyzing four benchmark instances (two applications using two different sets of parameters each) being executed on five datasets. We use execution time and energy consumption as the cost types for this investigation.
Research on a Support System for Automatic Ship Navigation in Fairway
Authors: Nguyen Van Suong
Published: February 2019

In previous investigations, controllers for the track-keeping of ships were designed with the assumption of constant ship speed. However, when navigating in a fairway area, the ship’s speed is usually decreased to prepare for berthing. The existing track-keeping systems, which are applied when the ship navigates in the open sea with a constant ship speed, cannot be used to navigate the ship in the fairway. In this article, a support system is proposed for ship navigation in the fairway. This system performs three tasks. First, the ship is automatically controlled by regulating the rudder to follow planned tracks. Second, the ship’s speed is reduced step by step to approach the berth area at a low speed. Finally, at low speed, when the ship’s rudder is not effective enough to control the ship’s heading to a desired angle, the ship’s heading is adjusted appropriately by the bow thruster before changing the control mode into the automatic berthing system. By the proposed system, the automatic systems can be combined to obtain a fully automatic system for ship control. To validate the effectiveness of this proposed system for automatic ship navigation in the fairway, numerical simulations were conducted with a training ship model.
The Effects of Quantitative Easing Announcements on the Mortgage Market: An Event Study Approach
Authors: Wang Gang
Published: February 2019

This paper uses event study analysis to estimate the impact of the United States Federal Reserve Bank’s (Fed) quantitative easing (QE) announcements on the mortgage market during the zero lower bound (ZLB) period. A total of 35 QE announcements are identified and their effects are evaluated. The best-fitting integrated generalized autoregressive conditional heteroskedasticity (IGARCH) model with skewed t distribution is used to measure the QE announcement effects on daily changes of the 30-year mortgage rate, the 30-year Treasury rate and the spread between them. Announcements suggesting the start of a new round of QE reduced the mortgage rate tremendously, while the effects of further news diminished. Announcements of an increase in mortgage-backed security purchases decreased the mortgage rate more than the Treasury rate and reduced the credit risk of holding mortgage securities over Treasury securities. The delayed effects of QE announcements on the mortgage rate were less than short-run effects but persistent. We also find that the previous literature overestimates QE effects on interest rates in general.
Cyclodextrin–Drug Inclusion Complexes: In Vivo and In Vitro Approaches
Authors: Carneiro Simone , Costa Duarte Fernanda , Heimfarth Luana , Siqueira Quintans Jullyana , Quintans-Júnior Lucindo , Veiga Júnior Valdir , Neves de Lima Ádley
Published: February 2019

This review aims to provide a critical review of the biological performance of natural and synthetic substances complexed with cyclodextrins, highlighting: (i) inclusion complexes with cyclodextrins and their biological studies in vitro and in vivo; (ii) Evaluation and comparison of the bioactive efficacy of complexed and non-complexed substances; (iii) Chemical and biological performance tests of inclusion complexes, aimed at the development of new pharmaceutical products. Based on the evidence presented in the review, it is clear that cyclodextrins play a vital role in the development of inclusion complexes which promote improvements in the chemical and biological properties of the complexed active principles, as well as providing improved solubility and aqueous stability. Although the literature shows the importance of their ability to help produce innovative biotechnological substances, we still need more studies to develop and expand their therapeutic properties. It is, therefore, very important to gather together evidence of the effectiveness of inclusion complexes with cyclodextrins in order to facilitate a better understanding of research on this topic and encourage further studies.
Potential for Drug Repositioning of Midazolam for Dentin Regeneration
Authors: Karakida Takeo , Onuma Kazuo , Saito Mari M. , Yamamoto Ryuji , Chiba Toshie , Chiba Risako , Hidaka Yukihiko , Fujii-Abe Keiko , Kawahara Hiroshi , Yamakoshi Yasuo
Published: February 2019

Drug repositioning promises the advantages of reducing costs and expediting approval schedules. An induction of the anesthetic and sedative drug; midazolam (MDZ), regulates inhibitory neurotransmitters in the vertebrate nervous system. In this study we show the potential for drug repositioning of MDZ for dentin regeneration. A porcine dental pulp-derived cell line (PPU-7) that we established was cultured in MDZ-only, the combination of MDZ with bone morphogenetic protein 2, and the combination of MDZ with transforming growth factor-beta 1. The differentiation of PPU-7 into odontoblasts was investigated at the cell biological and genetic level. Mineralized nodules formed in PPU-7 were characterized at the protein and crystal engineering levels. The MDZ-only treatment enhanced the alkaline phosphatase activity and mRNA levels of odontoblast differentiation marker genes, and precipitated nodule formation containing a dentin-specific protein (dentin phosphoprotein). The nodules consisted of randomly oriented hydroxyapatite nanorods and nanoparticles. The morphology, orientation, and chemical composition of the hydroxyapatite crystals were similar to those of hydroxyapatite that had transformed from amorphous calcium phosphate nanoparticles, as well as the hydroxyapatite in human molar dentin. Our investigation showed that a combination of MDZ and PPU-7 cells possesses high potential of drug repositioning for dentin regeneration.
Signaling Crosstalk between Salicylic Acid and Ethylene/Jasmonate in Plant Defense: Do We Understand What They Are Whispering?
Authors: Li Ning , Han Xiao , Feng Dan , Yuan Deyi , Huang Li-Jun
Published: February 2019

During their lifetime, plants encounter numerous biotic and abiotic stresses with diverse modes of attack. Phytohormones, including salicylic acid (SA), ethylene (ET), jasmonate (JA), abscisic acid (ABA), auxin (AUX), brassinosteroid (BR), gibberellic acid (GA), cytokinin (CK) and the recently identified strigolactones (SLs), orchestrate effective defense responses by activating defense gene expression. Genetic analysis of the model plant Arabidopsis thaliana has advanced our understanding of the function of these hormones. The SA- and ET/JA-mediated signaling pathways were thought to be the backbone of plant immune responses against biotic invaders, whereas ABA, auxin, BR, GA, CK and SL were considered to be involved in the plant immune response through modulating the SA-ET/JA signaling pathways. In general, the SA-mediated defense response plays a central role in local and systemic-acquired resistance (SAR) against biotrophic pathogens, such as Pseudomonas syringae, which colonize between the host cells by producing nutrient-absorbing structures while keeping the host alive. The ET/JA-mediated response contributes to the defense against necrotrophic pathogens, such as Botrytis cinerea, which invade and kill hosts to extract their nutrients. Increasing evidence indicates that the SA- and ET/JA-mediated defense response pathways are mutually antagonistic.
Cold Response of the Mediterranean Fruit Fly (Ceratitis capitata) on a Lab Diet
Authors: Al-Behadili Farhan J.M. , Bilgi Vineeta , Li Junxi , Wang Penghao , Taniguchi Miyuki , Agarwal Manjree , Ren Yonglin , Xu Wei
Published: February 2019

Cold treatment at 0.0 °C with different exposure durations (0–12 days) was applied to the Mediterranean fruit fly Ceratitis capitata (Wiedemann) fed on a lab diet. The examined developmental stages were early eggs (<6 h), late eggs (>42 h), first instar, second instar and third instar larvae. Pupation, adult emergence and sex ratios of survived flies were investigated to study the C. capitata responses to this low temperature treatment. Our results showed that exposure time at low temperature has a clear effect on pupation and adult emergence. Based on pupation ratios, the first and third instar are the most cold tolerant stages, with LT99 = 7.3 for both of them. Cold tolerance at both stages are very close and no significant differences were detected. There were no significant differences on C. capitata sex ratios among different stages after treatment. This study improves our understanding of C. capitata responses to cold treatment, which may assist in the improvement of the current treatment strategies to control this destructive horticulture pest species.
Temporal Change of Extracellular Matrix during Vein Arterialization Remodeling in Rats
Authors: Miyakawa Ayumi Aurea , Bassaneze Vinícius , Duarte Nubia Esteban , Girão-Silva Thais , Bizerra Monica Nunes , Campos Julliana Carvalho , Krieger Jose Eduardo
Published: February 2019

The global expression profile of the arterialized rat jugular vein was established to identify candidate genes and cellular pathways underlying the remodeling process. The arterialized jugular vein was analyzed on days 3 and 28 post-surgery and compared with the normal jugular vein and carotid artery. A gene array platform detected 9846 genes in all samples. A heatmap analysis uncovered patterns of gene expression showing that the arterialized vein underwent a partial transition from vein to artery from day 3 to 28 post-surgery. The same pattern was verified for 1845 key differentially expressed genes by performing a pairwise comparison of the jugular vein with the other groups. Interestingly, hierarchical clustering of 60 genes with altered expression on day 3 and day 28 displayed an expression pattern similar to that of the carotid artery. Enrichment analysis results and the network relationship among genes modulated during vein arterialization showed that collagen might play a role in the early remodeling process. Indeed, the total collagen content was increased, with the augmented expression of collagen I, collagen IV, and collagen V in arterialized veins. Additionally, there was an increase in the expression of versican and Thy-1 and a decrease in the expression of biglycan and β1-integrin. Overall, we provide evidence that vein arterialization remodeling is accompanied by consistent patterns of gene expression and that collagen may be an essential element underlying extracellular matrix changes that support the increased vascular wall stress of the new hemodynamic environment.
Peptic Ulcer Disease: A Brief Review of Conventional Therapy and Herbal Treatment Options
Authors: Kuna Lucija , Jakab Jelena , Smolic Robert , Raguz-Lucic Nikola , Vcev Aleksandar , Smolic Martina
Published: February 2019

Peptic ulcer is a chronic disease affecting up to 10% of the world’s population. The formation of peptic ulcers depends on the presence of gastric juice pH and the decrease in mucosal defenses. Non-steroidal anti-inflammatory drugs (NSAIDs) and Helicobacter pylori (H. pylori) infection are the two major factors disrupting the mucosal resistance to injury. Conventional treatments of peptic ulcers, such as proton pump inhibitors (PPIs) and histamine-2 (H2) receptor antagonists, have demonstrated adverse effects, relapses, and various drug interactions. On the other hand, medicinal plants and their chemical compounds are useful in the prevention and treatment of numerous diseases. Hence, this review presents common medicinal plants that may be used for the treatment or prevention of peptic ulcers.
Experimental Study of a Hybrid Wave Energy Converter Integrated in a Harbor Breakwater
Authors: Rosa-Santos Paulo , Taveira-Pinto Francisco , Clemente Daniel , Cabral Tomás , Fiorentin Felipe , Belga Filipe , Morais Tiago
Published: February 2019

Sea ports are infrastructures with substantial energy demands and often responsible for air pollution and other environmental problems, which may be minimized by using renewable energy, namely electricity harvested from ocean waves. In this regard, a wide variety of concepts to harvest wave energy are available and some shoreline technologies are already in an advanced development phase. The SE@PORTS project aims to assess the suitability and viability of existing wave energy conversion technologies to be integrated in harbor breakwaters, in order to take advantage of their high exposure to ocean waves. This paper describes the experimental study carried out to assess the performance of a hybrid wave energy converter (WEC) integrated in the rubble-mound structure that was proposed for the extension of the North breakwater of the Port of Leixões, Portugal. The hybrid concept combines the overtopping and the oscillating water column principles and was tested on a geometric scale of 1/50. This paper is focused on the assessment of the effects of the hybrid WEC integration on the case-study breakwater, both in terms of its stability and functionality. The 2D physical model included the reproduction of the seabed bathymetry in front of the breakwater and the generation of a wide range of irregular sea states, including extreme wave conditions. The experimental results shown that the integration of the hybrid WEC in the breakwater does not worsens the stability of its toe berm blocks and reduces the magnitude of the overtopping events. The conclusions obtained are therefore favorable to the integration of this type of devices on harbor breakwaters.
New Equations to Evaluate Lateral Displacement Caused by Liquefaction Using the Response Surface Method
Authors: Pirhadi Nima , Tang Xiaowei , Yang Qing
Published: February 2019

Few empirical and semi-empirical approaches have considered the influence of the geology, tectonic source, causative fault type, and frequency content of earthquake motion on lateral displacement caused by liquefaction (DH). This paper aims to address this gap in the literature by adding an earthquake parameter of the standardized cumulative absolute velocity (CAV5) to the original dataset for analyzing. Furthermore, the complex influence of fine content in the liquefiable layer (F15) is analyzed by deriving two different equations: the first one is for the whole range of parameters, and the second one is for a limited range of F15 values under 28% in order to the F15’s critical value presented in literature. The new response surface method (RSM) approach is applied on the basis of the artificial neural network (ANN) model to develop two new equations. Moreover, to illustrate the capability and efficiency of the developed models, the results of the RSM models are examined by comparing them with an additional three available models using data from the Chi-Chi earthquake sites that were not used for developing the models in this study. In conclusion, the RSM provides a capable tool to evaluate the liquefaction phenomenon, and the results fully justify the complex effect of different values of F15.
What Awaits Myanmar’s Uplands Farmers? Lessons Learned from Mainland Southeast Asia
Authors: Jepsen Martin Rudbeck , Palm Matilda , Bruun Thilde Bech
Published: February 2019

Mainland Southeast Asia (MSA) has seen sweeping upland land use changes in the past decades, with transition from primarily subsistence shifting cultivation to annual commodity cropping. This transition holds implications for local upland communities and ecosystems. Due to its particular political regime, Myanmar is at the tail of this development. However, with Myanmar’s official strategy of agricultural commercialization and intensification, recent liberalization of the national economy, and influx of multinational agricultural companies, the effects on upland land transitions could come fast. We analyze the current state of upland land use in Myanmar in a socio-economic and political context, identify the dynamics in three indicator commodity crops (maize, cassava, and rubber), and discuss the state driven economic, tenurial and policy reforms that have occurred in upland areas of mainland Southeast Asian countries in past decades. We draw on these insights to contextualize our study and hypothesize about possible transition pathways for Myanmar. The transition to annual commodity cropping is generally driven by a range of socio-economic and technical factors. We find that land use dynamics for the three indicator crops are associated with market demand and thus the opening of national Southeast-Asian economies, research and development of locally suitable high yielding varieties (HYVs), and subsidies for the promotion of seeds and inputs. In contrast, promotion of HYVs in marginal areas and without adequate agricultural extension services may results in agricultural contraction and yield dis-intensification. The environmental impacts of the transition depend on the transition pathway, e.g., through large-scale plantation projects or smallholder initiatives. The agricultural development in upland MSA follows a clear diffusion pattern with transition occurring first in Thailand, spreading to Vietnam, Cambodia and Laos. While these countries point to prospects for Myanmar, we hypothesize that changes will come slow due to Myanmar’s sparse rural infrastructure, with uncertainty about tenure, in particular in areas still troubled by armed conflicts, and unwillingness of international investors to approach Myanmar given the recent setbacks to the democratization process.
Improvement of ZrC/Zr Coating on the Interface Combination and Physical Properties of Diamond-Copper Composites Fabricated by Spark Plasma Sintering
Authors: Pan Yanpeng , He Xinbo , Ren Shubin , Wu Mao , Qu Xuanhui
Published: February 2019

In this study, diamond-copper composites were prepared with ZrC/Zr-coated diamond powders by spark plasma sintering. The magnetron sputtering technique was employed to coat the diamond particles with a zirconium layer. After heat treatment, most of the zirconium reacted with the surface of diamond and was transformed into zirconium carbide. The remaining zirconium on the zirconium carbide surface formed the outer layer. Owing to the method used to produce the ZrC/Zr-coated diamond in this study, the maximum thermal conductivity (TC) of 609 W·m−1·K−1 was obtained for 60 vol. % diamond-copper composites and the corresponding coefficient of thermal expansion (CTE) reached as low as 6.75 × 10−6 K−1. The bending strength of 40 vol. % ZrC/Zr-coated diamond-copper composites reached 255.95 MPa. The thermal and mechanical properties of ZrC/Zr-coated diamond-copper composites were substantially superior to those of uncoated diamond particles. Excellent properties can be attributed to the strengthening of the interfacial combination and the decrease in the interfacial thermal resistance due to the improvement associated with the ZrC/Zr coating. Theoretical analysis was also proposed to compare the thermal conductivities and CTE of diamond-copper composites fabricated with these two kinds of diamond powders.
A Probabilistic Model for Crystal Growth Applied to Protein Deposition at the Microscale
Authors: Bolos Vicente J. , Benitez Rafael , Eleta-Lopez Aitziber , Toca-Herrera Jose L.
Published: February 2019

A probabilistic discrete model for 2D protein crystal growth is presented. This model takes into account the available space and can describe growing processes of a different nature due to the versatility of its parameters, which gives the model great flexibility. The accuracy of the simulation is tested against a real recrystallization experiment, carried out with the bacterial protein SbpA from Lysinibacillus sphaericus CCM2177, showing high agreement between the proposed model and the actual images of the crystal growth. Finally, it is also discussed how the regularity of the interface (i.e., the curve that separates the crystal from the substrate) affects the evolution of the simulation.
Neorogioltriol and Related Diterpenes from the Red Alga Laurencia Inhibit Inflammatory Bowel Disease in Mice by Suppressing M1 and Promoting M2-Like Macrophage Responses
Authors: Daskalaki Maria G. , Vyrla Dimitra , Harizani Maria , Doxaki Christina , Eliopoulos Aristides G. , Roussis Vassilios , Ioannou Efstathia , Tsatsanis Christos , Kampranis Sotirios C.
Published: February 2019

Macrophages are central mediators of inflammation, orchestrating the inflammatory response through the production of cytokines and nitric oxide. Macrophages obtain pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes, which can be modulated by soluble factors, including natural products. Despite the crucial protective role of inflammation, chronic or deregulated inflammation can lead to pathological states, such as autoimmune diseases, metabolic disorders, cardiovascular diseases, and cancer. In this case, we studied the anti-inflammatory activity of neorogioltriol (1) in depth and identified two structurally related diterpenes, neorogioldiol (2), and O11,15-cyclo-14-bromo-14,15-dihydrorogiol-3,11-diol (3), with equally potent activity. We investigated the mechanism of action of metabolites 1–3 and found that all three suppressed macrophage activation and promoted an M2-like anti-inflammatory phenotype by inducing expression of Arginase1, MRC1, IRAK-M, the transcription factor C/EBPβ, and the miRNA miR-146a. In addition, they suppressed iNOS induction and nitric oxide production. Importantly, treatment of mice with 2 or 3 suppressed DSS-induced colitis by reducing tissue damage and pro-inflammatory cytokine production. Thus, all these three diterpenes are promising lead molecules for the development of anti-inflammatory agents targeting macrophage polarization mechanisms.
Models for Facilitated Transport Membranes: A Review
Authors: Rea Riccardo , De Angelis Maria , Baschetti Marco
Published: February 2019

Facilitated transport membranes are particularly promising in different separations, as they are potentially able to overcome the trade-off behavior usually encountered in solution-diffusion membranes. The reaction activated transport is a process in which several mechanisms take place simultaneously, and requires a rigorous theoretical analysis, which unfortunately is often neglected in current studies more focused on material development. In this work, we selected and reviewed the main mathematical models introduced to describe mobile and fixed facilitated transport systems in steady state conditions, in order to provide the reader with an overview of the existing mathematical tools. An analytical solution to the mass transport problem cannot be achieved, even when considering simple reaction schemes such as that between oxygen (solute) and hemoglobin (carrier) ( A + C ⇄ A C ), that was thoroughly studied by the first works dealing with this type of biological facilitated transport. Therefore, modeling studies provided approximate analytical solutions and comparison against experimental observations and exact numerical calculations. The derivation, the main assumptions, and approximations of such modeling approaches is briefly presented to assess their applicability, precision, and flexibility in describing and understanding mobile and fixed site carriers facilitated transport membranes. The goal is to establish which mathematical tools are more suitable to support and guide the development and design of new facilitated transport systems and materials. Among the models presented, in particular, those from Teramoto and from Morales-Cabrera et al. seem the more flexible and general ones for the mobile carrier case, while the formalization made by Noble and coauthors appears the most complete in the case of fixed site carrier membranes.
Influence of Porosity on the Elastic Modulus of Ti-Zr-Ta-Nb Foams with a Low Nb Content
Authors: Aguilar Claudio , Arancibia Mariette , Alfonso Ismeli , Sancy Mamie , Tello Karem , Salinas Vicente , De Las Cuevas Fernando
Published: February 2019

The development of titanium foams with a low elastic modulus has increased their scientific and technological relevance due to the evident need to avoid stress shielding problems. In this work, we studied the synthesis and characterization of Ti-13Zr-13Ta-3Nb (wt.%) alloy foams which present high potential for biomedical applications. A Ti-Nb-Ta-Zr mixture was produced by mechanical alloying using a planetary mill. Ti alloy foams were obtained using NaCl as a space-holder (40, 50, and 60 v/v %) that was mixed with the metallic powders and compacted under 420 MPa stress. NaCl particles were removed from the green compacts by submerging samples in distilled water at 60 °C. The green compacts were sintered at 1300 °C for 3 h in Ar atmosphere. Powders and foams were characterized by SEM and optical microscopy. The results showed that Ti-based foams with a tailored heterogeneous pore distribution can be obtained using the space holder method. The elastic modulus (E) of foams was estimated and measured between 5 and 25 GPa using theoretical and finite element analysis (FEA) models which are close to the E values measured experimentally. The results showed that foams with 50% and 60% porosity are potential bone replacement materials because their E value is closer to the E value of human bone.
Influence of Copper-Sided Tin Coating on the Weldability and Formation of Friction Stir Welded Aluminum-Copper-Joints
Authors: Eslami Nima , Hischer Yannik , Harms Alexander , Lauterbach Dennis , Böhm Stefan
Published: February 2019

Although the joining of aluminum and copper is a difficult task, several studies have shown that friction stir welding (FSW) is capable of producing aluminum-copper-joints with excellent performance. Therefore, it is desirable to use this joining technique for the production of cost- and weight-reduced conductors for the automotive sector. The exposed copper contact spots in automobiles are usually coated with tin for design reasons and in order to improve their corrosion resistance. In this context, it is possible to perform the weld at first and to coat afterwards, or to weld already coated copper workpieces. Taking this into account, this paper presents results on the influence of copper-sided tinning on the joint formation as well as the achievable mechanical and electrical properties of friction stir butt welded aluminum-copper joints. Two variants were considered. The first variant included copper blanks with a tinned surface. For the second variant the surface and the abutting edge of the copper were coated. The best welds achieved excellent electrical properties and their tensile strength was only slightly reduced compared to the aluminum base material. Thus, it was shown that if these tensile strength losses are acceptable, FSW of aluminum to tin coated copper is applicable.
A Fatigue Life Prediction Model Based on Modified Resolved Shear Stress for Nickel-Based Single Crystal Superalloys
Authors: Wang Jialiang , Wei Dasheng , Wang Yanrong , Jiang Xianghua
Published: February 2019

In this paper, the viewpoint that maximum resolved shear stress corresponding to the two slip systems in a nickel-based single crystal high-temperature fatigue experiment works together was put forward. A nickel-based single crystal fatigue life prediction model based on modified resolved shear stress amplitude was proposed. For the four groups of fatigue data, eight classical fatigue life prediction models were compared with the model proposed in this paper. Strain parameter is poor in fatigue life prediction as a damage parameter. The life prediction results of the fatigue life prediction model with stress amplitude as the damage parameter, the fatigue life prediction model with maximum resolved shear stress in 30 slip directions as the damage parameter, and the McDiarmid (McD) model, are better. The model proposed in this paper has higher life prediction accuracy.
Columbianadin Suppresses Lipopolysaccharide (LPS)-Induced Inflammation and Apoptosis through the NOD1 Pathway
Authors: Zhang Chao , Hsu Alan , Pan He , Gu Yinuo , Zuo Xu , Dong Bing , Wang Ziyan , Zheng Jingtong , Lu Junying , Zheng Ruipeng , Wang Fang
Published: February 2019

Columbianadin (CBN) is one of the main bioactive constituents isolated from the root of Angelica pubescens. Although the anti-inflammatory activity of CBN has been reported, the underpinning mechanism of this remains unclear. In this study, we investigated the anti-inflammatory effect of CBN on lipopolysaccharide (LPS)-stimulated THP-1 cells and explored the possible underlying molecular mechanisms. The results showed that CBN suppressed LPS-mediated inflammatory response mainly through the inactivation of the NOD1 and NF- κ B p65 signaling pathways. Knockdown of NOD1 reduced the degree to which inflammatory cytokines decreased following CBN treatment, whereas forced expression of NOD1 and CBN treatment reduced NF- κ B p65 activation and the secretion of inflammatory cytokines. Furthermore, CBN significantly reduced cellular apoptosis by inhibiting the NOD1 pathway. Collectively, our results indicate that CBN suppressed the LPS-mediated inflammatory response by inhibiting NOD1/NF- κ B activation. Further investigations are required to determine the mechanisms of action of CBN in the inhibition of NOD signaling: However, CBN may be employed as a therapeutic agent for multiple inflammatory diseases.
Ti-V-C-Based Alloy with a FCC Lattice Structure for Hydrogen Storage
Authors: Li Bo , He Liqing , Li Jianding , Li Hai-Wen , Lu Zhouguang , Shao Huaiyu
Published: February 2019

Here we report a Ti50V50-10 wt.% C alloy with a unique lattice and microstructure for hydrogen storage development. Different from a traditionally synthesized Ti50V50 alloy prepared by a melting method and having a body-centered cubic (BCC) structure, this Ti50V50-C alloy synthesized by a mechanical alloying method is with a face-centered cubic (FCC) structure (space group: Fm-3m No. 225). The crystalline size is 60 nm. This alloy may directly absorb hydrogen near room temperature without any activation process. Mechanisms of the good kinetics from lattice and microstructure aspects were discussed. Findings reported here may indicate a new possibility in the development of future hydrogen storage materials.
Biocompatibility Profile and In Vitro Cellular Uptake of Self-assembled Alginate Nanoparticles
Authors: Zhang Pei , Zhao Shirui , Yu Yaoyao , Wang Huan , Yang Yan , Liu Chenguang
Published: February 2019

Polymeric nanoparticles could offer promising controlled drug delivery. The biocompatibility is of extreme importance for future applications in humans. Self-assembled polymeric nanoparticles based on phenylalanine ethyl ester (PAE)-modified alginate (Alg) had been successfully prepared and characterized in our lab. However, little is known about their interaction with cells and other biological systems. In this study, nanoparticles (NPs) based on PAE-Alg conjugates (PEA-NPs) with different degree of substitution (DS) were prepared and investigated. Our results showed that PEA-NPs had no effects on the proliferation of the human intestinal epithelial Caco-2 cells at concentrations up to 1000 μg/mL. Furthermore, the in vitro cellular uptake profile of PEA-NPs, concerning several parameters involved in the application of therapeutic or diagnostic NPs, such as NPs concentration, time and temperature, was described. Different NPs have been adopted for cellular uptake studies and the NPs internalized into Caco-2 cells were quantified. Cellular uptake efficiency could reach 60% within 4 h. PEA-NPs also showed greater cell permeability than oleoyl alginate ester nanoparticles (OAE-NPs) previously prepared in our lab. Our studies reveal that NPs based on PEA conjugate are promising nanosystems for cellular delivery.
Effects of Pulsed Electric Field Treatment on Compression Properties and Solutes Diffusion Behaviors of Jerusalem artichoke
Authors: Zhu Zhenzhou , Zhang Rui , Grimi Nabil , Vorobiev Eugene
Published: February 2019

Jerusalem artichoke is widely used as raw material for industrial production of inulin. Pressing (compression) and diffusion are two effective technologies for bio-compounds’ recovery from plants. In this work, pulsed electric field (PEF) treatment at 400, 600, and 800 V/cm during 100 ms was applied to facilitate juice and solutes recovery from Jerusalem artichoke. The application of PEF led to electroporation of cell membranes and enhanced the tissue compression/juice expression and solutes diffusion. The consolidation coefficient (calculated by application of semi-empirical model) of PEF treated sample at 800 V/cm was 6.50 × 10−7 m2/s, which is significantly higher than that of untreated sample (5.02 × 10−9 m2/s) and close to that of freeze-thawed sample. Diffusion experiments with PEF treated samples were carried out at 25, 50, and 75 °C. A PEF treatment of Jerusalem artichoke at 800 V/cm led to a similar diffusion behavior at 25 °C, compared to diffusion behavior obtained from untreated sample at 75 °C.
Design, Synthesis, and Biological Activities of Novel 1,3,5-Trimethylpyrazole-Containing Malonamide Derivatives
Authors: Li Qi-Bo , Liao Min , Liu Qing , Feng Tong , Xu Zhi-Yuan , Rui Chang-Hui , Liu Shang-Zhong
Published: February 2019

New 1,3,5-trimethylpyrazole-containing malonamide derivatives based on pyflubumide were designed, synthesized, and characterized using 1H-NMR, 13C-NMR, and high-resolution mass spectra (HRMS). The results of preliminary bioassays showed that the target compounds possessed good activities against Tetranychus cinnabarinus, Plutella xylostella, and Aphis craccivora. Most of the target compounds exhibited moderate to good acaricidal activity against Tetranychus cinnabarinus at a concentration of 400 µg/mL, and some showed moderate activity at a concentration of 200 µg/mL; in particular, compounds 8m and 8p exhibited 70.0% mortality. In addition, some of the target compounds exhibited good insecticidal activities against Plutella xylostella at a concentration of 200 µg/mL, especially compounds 8i and 8o, which achieved 100.0% mortality at a concentration of 100 µg/mL. Interestingly, some of the target compounds exhibited potent anti-aphid activity against Aphis craccivora at a concentration of 200 µg/mL; furthermore, compounds 8p and 8q demonstrated 100.0% anti-aphid activity at a concentration of 50 µg/mL. The preliminary analyses of the structure–activity relationships (SAR) indicated that the acaricidal and insecticidal activities varied significantly depending on the type of substituent and substitution pattern, which provides guidance for the further investigation of such structural modifications.
Investigation of Permeation of Theophylline through Skin Using Selected Piperazine-2,5-Diones
Authors: Pokorna Aneta , Bobal Pavel , Oravec Michal , Rarova Lucie , Bobalova Janette , Jampilek Josef
Published: February 2019

Transdermal administration of drugs that penetrate, in this case directly into the blood circulation, has many advantages and is promising for many drugs thanks to its easy application and good patient compliance. (S)-8-Methyl-6,9-diazaspiro[4.5]decan-7,10-dione (alaptide), has been studied as a potential chemical permeation enhancer. Based on its structure, four selected piperazine-2,5-diones were synthesized by means of multi-step synthetic pathways. All the compounds were investigated on their ability to enhance the permeation of the model drug theophylline from the hydrophilic medium propylene glycol:water (1:1). In vitro experiments were performed using vertical Franz diffusion cells at constant temperature 34 ± 0.5 °C and using full-thickness pig (Sus scrofa f. domestica) ear skin. Withdrawn samples were analyzed by RP-HPLC for determination of the permeated amount of theophylline. All the compounds were applied in ratio 1:10 (w/w) relative to the amount of theophylline. One hour after application, the permeated amount of theophylline from formulations with alaptide and (3S,6S)-3,6-dimethylpiperazine-2,5-dione, was ca. 15- and 12-fold higher, respectively, than from the formulation without the tested compounds. Despite the enhancement ratio of both enhancers in a steady state was ca. 2.3, the pseudo-enhancement ratio in the time range from 1 to 3 h was 4.4. These enhancement ratios indicate that the compounds are able to enhance the permeation of agents through the skin; however, the short-term application of both compound formulations seems to be more advantageous. In addition, the screening of the cytotoxicity of all the prepared compounds was performed using three cell lines, and the compounds did not show any significant toxic effect.
Comparison of Bioactive Compounds and Antioxidant Activities of Maclura tricuspidata Fruit Extracts at Different Maturity Stages
Authors: Kim Dae-Woon , Lee Won-Jae , Asmelash Gebru Yoseph , Choi Han-Seok , Yeo Soo-Hwan , Jeong Young-Jae , Kim Seung , Kim Young-Hoi , Kim Myung-Kon
Published: February 2019

Maclura tricuspidata fruit contains various bioactive compounds and has traditionally been used in folk medicine and as valuable food material in Korea. The composition and contents of bioactive compounds in the fruit can be influenced by its maturity stages. In this study, total phenol, total flavonoid, individual polyphenolic compounds, total carotenoids and antioxidant activities at four maturity stages of the fruit were determined. Polyphenolic compounds were analyzed using high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) and HPLC. Among 18 polyphenolic compounds identified in this study, five parishin derivatives (gastrodin, parishin A, B, C, E) were positively identified for the first time in this plant. These compounds were also validated and quantified using authentic standards. Parishin A was the most abundant component, followed by chlorogenic acid, gastrodin, eriodictyol glucoside, parishin C, parishin E and parishin B. The contents of all the polyphenolic compounds were higher at the immature and premature stages than at fully mature and overmature stages, while total carotenoid was found to be higher in the mature and overmature stages. Overall antioxidant activities by three different assays (DPPH, ABTS, FRAP) decreased as maturation progressed. Antioxidant properties of the fruit extract are suggested to be attributed to the polyphenols.
An Example of Polynomial Expansion: The Reaction of 3(5)-Methyl-1H-Pyrazole with Chloroform and Characterization of the Four Isomers
Authors: Silva Vera L. M. , Silva Artur M. S. , Claramunt Rosa M. , Sanz Dionisia , Infantes Lourdes , Martínez-López Ángela , Reviriego Felipe , Alkorta Ibon , Elguero José
Published: February 2019

The reaction in phase-transfer catalyzed conditions of 3(5)-methyl-1H-pyrazole with chloroform affords four isomers 333, 335, 355 and 555 in proportions corresponding to the polynomial expansion (a + b)3, with a = 0.6 and b = 0.4, a and b being 3-methyl and 5-methyl proportions. The up (u) and down (d) conformation of the pyrazolyl rings with regard to the Csp3–H atom was established by X-ray crystallography and by 1H-, 13C- and 15N-NMR in solution combined with gauge-including atomic orbitals (GIAO)/B3LYP/6-311++G(d,p) calculations. A comparison with other X-ray structures of tris-pyrazolylmethanes was carried out.
Synthesis and Luminescence Properties of Core-Shell-Shell Composites: SiO2@PMDA-Si-Tb@SiO2 and SiO2@PMDA-Si-Tb-phen@SiO2
Authors: Feng Lina , Li Wenxian , Bao Jinrong , Zheng Yushan , Li Yilian , Ma Yangyang , Yang Kuisuo , Qiao Yan , Wu Anping
Published: February 2019

Two novel core-shell composites SiO2@PMDA-Si-Tb, SiO2@PMDA-Si-Tb-phen with SiO2 as the core and terbium organic complex as the shell, were successfully synthesized. The terbium ion was coordinated with organic ligand forming terbium organic complex in the shell layer. The bi-functional organosilane ((HOOC)2C6H2(CONH(CH2)3Si(OCH2CH3)3)2 (abbreviated as PMDA-Si) was used as the first ligand and phen as the second ligand. Furthermore, the silica-modified SiO2@PMDA-Si-Tb@SiO2 and SiO2@PMDA-Si-Tb-phen@SiO2 core-shell-shell composites were also synthesized by sol-gel chemical route. An amorphous silica shell was coated around the SiO2@PMDA-Si-Tb and SiO2@PMDA-Si-Tb-phen core-shell composites. The core-shell and core-shell-shell composites both exhibited excellent luminescence in solid state. The luminescence of core-shell-shell composites was stronger than that of core-shell composites. Meanwhile, an improved luminescence stability property for the core-shell-shell composites was found in the aqueous solution. The core-shell-shell composites exhibited bright luminescence, high stability, long lifetime, and good solubility, which may present potential applications in the bio-medical field.
Optical Sensors Based on II-VI Quantum Dots
Authors: Lesiak Anna , Drzozga Kamila , Cabaj Joanna , Bański Mateusz , Malecha Karol , Podhorodecki Artur
Published: February 2019

Fundamentals of quantum dots (QDs) sensing phenomena show the predominance of these fluorophores over standard organic dyes, mainly because of their unique optical properties such as sharp and tunable emission spectra, high emission quantum yield and broad absorption. Moreover, they also indicate no photo bleaching and can be also grown as no blinking emitters. Due to these properties, QDs may be used e.g., for multiplex testing of the analyte by simultaneously detecting multiple or very weak signals. Physico-chemical mechanisms used for analyte detection, like analyte stimulated QDs aggregation, nonradiative Förster resonance energy transfer (FRET) exhibit a number of QDs, which can be applied in sensors. Quantum dots-based sensors find use in the detection of ions, organic compounds (e.g., proteins, sugars, volatile substances) as well as bacteria and viruses.
First-Principles Study of Thermo-Physical Properties of Pu-Containing Gd2Zr2O7
Authors: Li Pengcheng , Zhao Fengai , Xiao Haiyan , Zhang Haibin , Gong Hengfeng , Zhang Sa , Liu Zijiang , Zu Xiaotao
Published: February 2019

A density functional theory plus Hubbard U method is used to investigate how the incorporation of Pu waste into Gd2Zr2O7 pyrochlore influences its thermo-physical properties. It is found that immobilization of Pu at Gd-site of Gd2Zr2O7 has minor effects on the mechanical and thermal properties, whereas substitution of Pu for Zr-site results in remarkable influences on the structural parameters, elastic moduli, elastic isotropy, Debye temperature and electronic structure. The discrepancy in thermo-physical properties between Gd2−yPuyZr2O7 and Gd2Zr2−yPuyO7 may be a result of their different structural and electronic structures. This study provides a direct insight into the thermo-physical properties of Pu-containing Gd2Zr2O7, which will be important for further investigation of nuclear waste immobilization by pyrochlores.
Are (All) Consumers Averse to Bitter Taste?
Authors: Vecchio Riccardo , Cavallo Carla , Cicia Gianni , Del Giudice Teresa
Published: February 2019

The current study combined hedonic liking with non-hypothetical experimental auctions to measure consumer preferences for bitter tasting food and identify individual socio-demographic and psychographic characteristics that influence bitter aversion. Furthermore, the research analyzed whether consumer preferences for bitter food were influenced by sensory and health-related information. Findings reveal that respondents (N = 205) are not averse to bitter taste; while, socio-demographic traits influence bitter acceptance, as higher education level and gender (female) positively affect preferences, together with specific individual characteristics as high compensatory health beliefs. Moreover, results prove that participants positively respond to health-related information, whereas information on bitterness-taste generates lower preferences.
Associations between the Level of Trace Elements and Minerals and Folate in Maternal Serum and Amniotic Fluid and Congenital Abnormalities
Authors: Kocylowski Rafal , Grzesiak Mariusz , Gaj Zuzanna , Lorenc Wiktor , Bakinowska Ewa , Barałkiewicz Danuta , von Kaisenberg Constantin S. , Lamers Yvonne , Suliburska Joanna
Published: February 2019

Congenital birth defects may result in a critical condition affecting the baby, including severe fetal/neonatal handicap and mortality. Several studies have shown that genetic, nutritional, and environmental factors may have an impact on fetal development and neonatal health. The relevance of essential and toxic elements on fetal development has not yet been fully investigated, and the results of recent research indicate that these elements may be crucial in the assessment of the risk of malformations in neonates. We determined the association between essential and toxic elements and the level of folate in maternal serum (MS) and amniotic fluid (AF), along with neonatal abnormalities. A total of 258 pregnant Polish women in the age group of 17–42 years participated in this study. AF and MS were collected during vaginal delivery or during cesarean section. An inductively coupled plasma mass spectrometry technique was used to determine the levels of various elements in AF and MS. The results of this exploratory study indicate that the levels of essential and toxic elements are associated with fetal and newborn anatomical abnormalities and growth disorders.