bluuebunny/crossref_metadata_2025 huggingface dataset:
Convenience-based food purchase patterns: identification and associations with dietary quality, sociodemographic factors and attitudes
Authors: Peltner Jonas , Thiele Silke
Published: November 2017

Objective

The present study aimed to derive food purchase patterns considering the convenience level of foods. Associations between identified patterns and dietary quality were analysed, as well as household characteristics associated with the dietary patterns.

Design

A Convenience Food Classification Scheme (CFCS) was developed. After classifying basic food groups into the CFCS, the formed groups were used to apply a factor analysis to identify convenience-based food purchase patterns. For these patterns nutrient and energy densities were examined. Using regression analysis, associations between the adherence to the patterns and household characteristic and attitude variables were analysed.

Subjects

The study used representative German food purchase data from 2011. Approximately 12 million purchases of 13 131 households were recorded in these data.

Results

Three convenience-based patterns were identified: a low-convenience, a semi-convenience and a ready-to-eat food pattern. Tighter adherence to the semi-convenience pattern was shown to result in the lowest nutrient and highest energy densities. Important factors influencing adherence to the patterns were household size, presence of children and attitudes. Working full-time was negatively associated with adherence to the low-convenience pattern and positively with the ready-to-eat pattern.

Conclusions

Convenience foods were an important part of households’ food baskets which in some cases led to lower nutritional quality. Therefore, it is important to offer convenience foods higher in nutrient density and lower in energy density. Interventions targeted on enhancing cooking skills could be an effective strategy to increase purchases of unprocessed foods, which, in turn, could also contribute to an improved diet quality.
Total scatter factor for small fields in radiotherapy: a dosimetric comparison
Authors: Shamsi Qurat-ul-ain , Ahmad Buzdar Saeed , Altaf Saima , Atiq Atia , Atiq Maria , Iqbal Khalid
Published: November 2017

Purpose

Small field dosimetry is complicated and accuracy in the measurement of total scatter factor (TSF) is crucial for dosimetric calculations, in making optimum intensity-modulated radiotherapy plans for treating small target volumes. In this study, we intended to determine the TSF measuring properties of CC01 and CC04 detectors for field sizes ranging from sub-centimetre to the centimetre fields.

Material and methods

CC01 and CC04 chamber detectors were used to measure TSF for 6 and 18 MV photon beam delivered from the linear accelerator, through small fields in a water phantom. Small fields were created by collimator jaws and multi-leaf collimators separately, with field sizes ranging from 0·6 to 10 cm2and 0·5 to 20 cm2, respectively.

Results

CC01 measured TSF at all the given field sizes created by jaws and multi-leaf collimators for both 6 and 18 MV beams whereas CC04 could not measure TSF for field sizes <1 cm2due to volume averaging and perturbation effects.

Conclusion

CC01 was shown to be effective for measurement of TSF in sub-centimetre field sizes. CC01 can be employed to measure other dosimetric quantities in small fields using different energy beams.
Portuguese culture and legal consciousness: a discussion of immigrant women's perceptions of and reactions to domestic violence
Authors: Graca Sofia
Published: November 2017

This paper uses legal consciousness to discuss the influence of Portuguese culture on women's perceptions of and reactions to domestic violence. It is based on an in-depth small-scale study of Portuguese women living in England, and proposes that culture is central in shaping their behaviour, regardless of whether they experienced violence or not. The cultural characteristics that influence women the most are analysed here under the themes of ‘familism’, ‘shame and community pressure’ and ‘acculturation’. These do not operate all at the same level and their influence can change according to structural and individual circumstances. As such, the paper suggests that immigrant women's perceptions of and reactions to domestic violence can only be fully understood by articulating national culture with other structural and individual variables; this will enable a multilayered and situated understanding of women's legality that avoids a simplistic attribution of their behaviour to national or ethnic provenance.
Simple or complex? A case study of physical and mental health co-morbidity
Authors: Bennett Sophie D. , Heyman Isobel , Varadkar Sophia , Coughtrey Anna E. , Shafran Roz
Published: November 2017

People with epilepsy are significantly more likely to have a mental health disorder than those without a chronic illness. The reasons for this are multiple but may include the mental health difficulties being perceived as complex due to the presence of a chronic illness. In part due to the apparent complexity of the co-occurring physical and mental illness, many are not offered evidence-based treatment (EBT) for the mental health disorder. There is little guidance to inform clinicians about the interventions to use to treat mental health disorders in people with epilepsy. The present paper reports a case of treatment for depression using a standard EBT in a young person with epilepsy. The patient also had clinically significant symptoms of anxiety and an eating disorder and would be considered ‘complex’ according to standard criteria. The intervention, however, was relatively simple and was delivered as guided self-help via 10 weekly telephone calls of approximately 30 minutes duration, and two follow-up calls at one month and three months post-intervention. Self-report and parent-report questionnaire measures were completed before and after the intervention, and at both follow-up time points. A blind-rated online diagnostic interview measure was also completed before and after the intervention. The young person and her family also completed a qualitative interview of their experiences of the intervention. This simple intervention was effective in working towards the client's goals, although pre–post measurement on standard measures was variable. This interesting case raises questions about whether patients with mental and physical comorbidities are complex, or just perceived as complex.
Oil, Islam, and the Middle East: An Empirical Analysis of the Repression of Religion, 1980–2013
Authors: Albertsen Daniel , de Soysa Indra
Published: November 2017

There is a lively debate on the relative impacts of Islam, oil wealth, and Middle Eastern institutional legacies regarding democratization and the spread of liberal values. We examine this issue using religious repression. We argue that oil-wealthy rulers use religious monopoly to control dissent. Our results show that oil wealth increases religious repression above the effects of Muslim dominance and a host of sundry controls. The Middle East and North Africa region seems to matter more than Islam. Interestingly, the conditional effect of oil and the Middle East and North Africa region is positive on religious freedom. The data suggest that several Gulf monarchies have more religious freedoms than other Muslim dominant states, such as Saudi Arabia and Iran, or even Israel and Jordan. The worst religious repression is among oil producers in Central Asia. The results are robust to a host of intervening factors, different measures of oil wealth, alternative data on religious freedom, and estimating method.
Epithelial-to-mesenchymal transition in gallbladder cancer: from clinical evidence to cellular regulatory networks
Authors: Xu Sunwang , Zhan Ming , Wang Jian
Published: November 2017

Gallbladder cancer (GBC), with late diagnosis, rapid disease progression and early metastasis, is a highly aggressive malignant tumor found worldwide. Patients with GBC have poor survival, low curative resection rates and early recurrence. For such a lethal tumor, uncovering the mechanisms and exploring new strategies to prevent tumor progression and metastasis are critically important. Epithelial-to-mesenchymal transition (EMT) has a prominent role in the early steps of tumor progression and metastasis by initiating polarized epithelial cell transition into motile mesenchymal cells. Accumulating evidence suggests that EMT can be modulated by the cooperation of multiple mechanisms affecting common targets. Signaling pathways, transcriptional and post-transcriptional regulation and epigenetic alterations are involved in the stepwise EMT regulatory network in GBC. Loss of epithelial markers, acquisition of mesenchymal markers and dysregulation of EMT-inducing transcription factors (EMT-TFs) have been observed and are associated with the clinicopathology and prognosis of GBC patients. Therefore, EMT may be a detectable and predictable event for predicting GBC progression and metastasis in the clinic. In this review, we will provide an overview of EMT from the clinical evidence to cellular regulatory networks that have been studied thus far in clinical and basic GBC studies.
Expressed fusion gene landscape and its impact in multiple myeloma
Authors: Cleynen A. , Szalat R. , Kemal Samur M. , Robiou du Pont S. , Buisson L. , Boyle E. , Chretien M. L. , Anderson K. , Minvielle S. , Moreau P. , Attal M. , Parmigiani G. , Corre J. , Munshi N. , Avet-Loiseau H.
Published: November 2017

Multiple myeloma is a plasma cell malignancy characterized by recurrent IgH translocations and well described genomic heterogeneity. Although transcriptome profiles in multiple myeloma has been described, landscape of expressed fusion genes and their clinical impact remains unknown. To provide a comprehensive and detailed fusion gene cartography and suggest new mechanisms of tumorigenesis in multiple myeloma, we performed RNA sequencing in a cohort of 255 newly diagnosed and homogeneously treated multiple myeloma patients with long follow-up. Here, we report that patients have on average 5.5 expressed fusion genes. Kappa and lambda light chains and IgH genes are main partners in a third of all fusion genes. We also identify recurrent fusion genes that significantly impact both progression-free and overall survival and may act as drivers of the disease. Lastly, we find a correlation between the number of fusions, the age of patients and the clinical outcome, strongly suggesting that genomic instability drives prognosis of the disease.
Schwann cell TRPA1 mediates neuroinflammation that sustains macrophage-dependent neuropathic pain in mice
Authors: De Logu Francesco , Nassini Romina , Materazzi Serena , Carvalho Gonçalves Muryel , Nosi Daniele , Rossi Degl’Innocenti Duccio , Marone Ilaria M. , Ferreira Juliano , Li Puma Simone , Benemei Silvia , Trevisan Gabriela , Souza Monteiro de Araújo Daniel , Patacchini Riccardo , Bunnett Nigel W. , Geppetti Pierangelo
Published: November 2017

It is known that transient receptor potential ankyrin 1 (TRPA1) channels, expressed by nociceptors, contribute to neuropathic pain. Here we show that TRPA1 is also expressed in Schwann cells. We found that in mice with partial sciatic nerve ligation, TRPA1 silencing in nociceptors attenuated mechanical allodynia, without affecting macrophage infiltration and oxidative stress, whereas TRPA1 silencing in Schwann cells reduced both allodynia and neuroinflammation. Activation of Schwann cell TRPA1 evoked NADPH oxidase 1 (NOX1)-dependent H2O2release, and silencing or blocking Schwann cell NOX1 attenuated nerve injury-induced macrophage infiltration, oxidative stress and allodynia. Furthermore, the NOX2-dependent oxidative burst, produced by macrophages recruited to the perineural space activated the TRPA1–NOX1 pathway in Schwann cells, but not TRPA1 in nociceptors. Schwann cell TRPA1 generates a spatially constrained gradient of oxidative stress, which maintains macrophage infiltration to the injured nerve, and sends paracrine signals to activate TRPA1 of ensheathed nociceptors to sustain mechanical allodynia.
Nematic superconducting state in iron pnictide superconductors
Authors: Li Jun , Pereira Paulo J. , Yuan Jie , Lv Yang-Yang , Jiang Mei-Ping , Lu Dachuan , Lin Zi-Quan , Liu Yong-Jie , Wang Jun-Feng , Li Liang , Ke Xiaoxing , Van Tendeloo Gustaaf , Li Meng-Yue , Feng Hai-Luke , Hatano Takeshi , Wang Hua-Bing , Wu Pei-Heng , Yamaura Kazunari , Takayama-Muromachi Eiji , Vanacken Johan , Chibotaru Liviu F. , Moshchalkov Victor V.
Published: November 2017

Nematic order often breaks the tetragonal symmetry of iron-based superconductors. It arises from regular structural transition or electronic instability in the normal phase. Here, we report the observation of a nematic superconducting state, by measuring the angular dependence of the in-plane and out-of-plane magnetoresistivity of Ba0.5K0.5Fe2As2 single crystals. We find large twofold oscillations in the vicinity of the superconducting transition, when the direction of applied magnetic field is rotated within the basal plane. To avoid the influences from sample geometry or current flow direction, the sample was designed as Corbino-shape for in-plane and mesa-shape for out-of-plane measurements. Theoretical analysis shows that the nematic superconductivity arises from the weak mixture of the quasi-degenerate s-wave and d-wave components of the superconducting condensate, most probably induced by a weak anisotropy of stresses inherent to single crystals.
Alternative activation generates IL-10 producing type 2 innate lymphoid cells
Authors: Seehus Corey R. , Kadavallore Asha , Torre Brian de la , Yeckes Alyson R. , Wang Yizhou , Tang Jie , Kaye Jonathan
Published: November 2017

Type 2 innate lymphoid cells (ILC2) share cytokine and transcription factor expression with CD4+Th2 cells, but functional diversity of the ILC2 lineage has yet to be fully explored. Here, we show induction of a molecularly distinct subset of activated lung ILC2, termed ILC210. These cells produce IL-10 and downregulate some pro-inflammatory genes. Signals that generate ILC210are distinct from those that induce IL-13 production, and gene expression data indicate that an alternative activation pathway leads to the generation of ILC210. In vivo, IL-2 enhances ILC210generation and is associated with decreased eosinophil recruitment to the lung. Unlike most activated ILC2, the ILC210population contracts after cessation of stimulation in vivo, with maintenance of a subset that can be recalled by restimulation, analogous to T-cell effector cell and memory cell generation. These data demonstrate the generation of a previously unappreciated IL-10 producing ILC2 effector cell population.
Metric clusters in evolutionary games on scale-free networks
Authors: Kleineberg Kaj-Kolja
Published: November 2017

The evolution of cooperation in social dilemmas in structured populations has been studied extensively in recent years. Whereas many theoretical studies have found that a heterogeneous network of contacts favors cooperation, the impact of spatial effects in scale-free networks is still not well understood. In addition to being heterogeneous, real contact networks exhibit a high mean local clustering coefficient, which implies the existence of an underlying metric space. Here we show that evolutionary dynamics in scale-free networks self-organize into spatial patterns in the underlying metric space. The resulting metric clusters of cooperators are able to survive in social dilemmas as their spatial organization shields them from surrounding defectors, similar to spatial selection in Euclidean space. We show that under certain conditions these metric clusters are more efficient than the most connected nodes at sustaining cooperation and that heterogeneity does not always favor—but can even hinder—cooperation in social dilemmas.
Bending and breaking of stripes in a charge ordered manganite
Authors: Savitzky Benjamin H. , El Baggari Ismail , Admasu Alemayehu S. , Kim Jaewook , Cheong Sang-Wook , Hovden Robert , Kourkoutis Lena F.
Published: November 2017

In charge-ordered phases, broken translational symmetry emerges from couplings between charge, spin, lattice, or orbital degrees of freedom, giving rise to remarkable phenomena such as colossal magnetoresistance and metal–insulator transitions. The role of the lattice in charge-ordered states remains particularly enigmatic, soliciting characterization of the microscopic lattice behavior. Here we directly map picometer scale periodic lattice displacements at individual atomic columns in the room temperature charge-ordered manganite Bi0.35Sr0.18Ca0.47MnO3 using aberration-corrected scanning transmission electron microscopy. We measure transverse, displacive lattice modulations of the cations, distinct from existing manganite charge-order models. We reveal locally unidirectional striped domains as small as ~5 nm, despite apparent bidirectionality over larger length scales. Further, we observe a direct link between disorder in one lattice modulation, in the form of dislocations and shear deformations, and nascent order in the perpendicular modulation. By examining the defects and symmetries of periodic lattice displacements near the charge ordering phase transition, we directly visualize the local competition underpinning spatial heterogeneity in a complex oxide.
Neuroprotective role of retinal SIRT3 against acute photo-stress
Authors: Ban Norimitsu , Ozawa Yoko , Osada Hideto , Lin Jonathan B. , Toda Eriko , Watanabe Mitsuhiro , Yuki Kenya , Kubota Shunsuke , Apte Rajendra S. , Tsubota Kazuo
Published: November 2017

SIRT3 is a key regulator of mitochondrial reactive oxygen species as well as mitochondrial function. The retina is one of the highest energy-demanding tissues, in which the regulation of reactive oxygen species is critical to prevent retinal neurodegeneration. Although previous reports have demonstrated that SIRT3 is highly expressed in the retina and important in neuroprotection, function of SIRT3 in regulating reactive oxygen species in the retina is largely unknown. In this study, we investigated the role of retinal SIRT3 in a light-induced retinal degeneration model using SIRT3 knockout mice. We demonstrate that SIRT3 deficiency causes acute reactive oxygen species accumulation and endoplasmic reticulum stress in the retina after the light exposure, which leads to increased photoreceptor death, retinal thinning, and decreased retinal function. Using a photoreceptor-derived cell line, we revealed that reactive oxygen species were the upstream initiators of endoplasmic reticulum stress. Under SIRT3 knockdown condition, we demonstrated that decreased superoxide dismutase 2 activity led to elevated intracellular reactive oxygen species. These studies have helped to elucidate the critical role of SIRT3 in photoreceptor neuronal survival, and suggest that SIRT3 might be a therapeutic target for oxidative stress-induced retinal disorders.
Network Properties in Transitions of Consciousness during Propofol-induced Sedation
Authors: Lee Minji , Sanders Robert D. , Yeom Seul-Ki , Won Dong-Ok , Seo Kwang-Suk , Kim Hyun Jeong , Tononi Giulio , Lee Seong-Whan
Published: November 2017

Reliable electroencephalography (EEG) signatures of transitions between consciousness and unconsciousness under anaesthesia have not yet been identified. Herein we examined network changes using graph theoretical analysis of high-density EEG during patient-titrated propofol-induced sedation. Responsiveness was used as a surrogate for consciousness. We divided the data into five states: baseline, transition into unresponsiveness, unresponsiveness, transition into responsiveness, and recovery. Power spectral analysis showed that delta power increased from responsiveness to unresponsiveness. In unresponsiveness, delta waves propagated from frontal to parietal regions as a traveling wave. Local increases in delta connectivity were evident in parietal but not frontal regions. Graph theory analysis showed that increased local efficiency could differentiate the levels of responsiveness. Interestingly, during transitions of responsive states, increased beta connectivity was noted relative to consciousness and unconsciousness, again with increased local efficiency. Abrupt network changes are evident in the transitions in responsiveness, with increased beta band power/connectivity marking transitions between responsive states, while the delta power/connectivity changes were consistent with the fading of consciousness using its surrogate responsiveness. These results provide novel insights into the neural correlates of these behavioural transitions and EEG signatures for monitoring the levels of consciousness under sedation.
The solvability of quantum k-pair network in a measurement-based way
Authors: Li Jing , Xu Gang , Chen Xiu-Bo , Qu Zhiguo , Niu Xin-Xin , Yang Yi-Xian
Published: November 2017

Network coding is an effective means to enhance the communication efficiency. The characterization of network solvability is one of the most important topic in this field. However, for general network, the solvability conditions are still a challenge. In this paper, we consider the solvability of general quantumk-pair network in measurement-based framework. For the first time, a detailed account of measurement-based quantum network coding(MB-QNC) is specified systematically. Differing from existing coding schemes, single qubit measurements on a pre-shared graph state are the only allowed coding operations. Since no control operations are concluded, it makes MB-QNC schemes more feasible. Further, the sufficient conditions formulating by eigenvalue equations and stabilizer matrix are presented, which build an unambiguous relation among the solvability and the general network. And this result can also analyze the feasibility of sharingkEPR pairs task in large-scale networks. Finally, in the presence of noise, we analyze the advantage of MB-QNC in contrast to gate-based way. By an instance network$${G}_{k}$$Gk, we show that MB-QNC allows higher error thresholds. Specially, forXerror, the error threshold is about 30% higher than 10% in gate-based way. In addition, the specific expressions of fidelity subject to some constraint conditions are given.
fMRI Investigation on Gradual Change of Awareness States in Implicit Sequence Learning
Authors: Huang Jianping , Li Yingli , Zhang Jianxin , Wang Xiangpeng , Huang Chunlu , Chen Antao , Liu Dianzhi
Published: November 2017

Awareness of implicit knowledge is a changing process. Previous studies have examined brain activation patterns corresponding to the start and end stages of implicit learning, but failed to reveal the gradual changing course of awareness in implicit learning. The present study explored brain activation changes corresponding to different awareness states elicited by two different stimulus onset asynchrony (SOA, 850 ms and 1350 ms) over the whole course of implicit sequence learning (i.e., divided into three phases), by using a process dissociation procedure (PDP) paradigm and the technique of functional magnetic resonance imaging (fMRI). In the results, it was found that the 850 ms SOA elicited primarily an awareness state of unconsciousness, under which the frontal lobe was significantly activated during the early phase of implicit sequence learning, with its activation levels correlated positively to consciousness levels. In contrast, the 1350 ms SOA triggered predominantly an awareness state of consciousness, under which the activation levels of the inferior parietal lobule correlated positively to consciousness levels during the middle phase, and positively to consciousness levels as well as negatively to unconsciousness levels during the late phase of implicit sequence learning. Overall, the frontal lobe and inferior parietal lobule were found to play critical roles in mediating awareness states over the course of implicit sequence learning.
Mimicking Embedded Vasculature Structure for 3D Cancer on a Chip Approaches through Micromilling
Authors: Wan L. , Skoko J. , Yu J. , Ozdoganlar O. B. , LeDuc P. R. , Neumann C. A.
Published: November 2017

The ability for cells to sense and respond to microenvironmental signals is influenced by their three dimensional (3D) surroundings, which includes the extracellular matrix (ECM). In the 3D environment, vascular structures supply cells with nutrients and oxygen thus affecting cell responses such as motility. Interpretation of cell motility studies though is often restricted by the applied approaches such as 2D conventional soft lithography methods that have rectangular channel cross-sectional morphology. To better simulate cell responses to vascular supply in 3D, we developed a cell on a chip system with microfluidic channels with curved cross-sections embedded within a 3D collagen matrix that emulates anatomical vasculature more closely than inorganic polymers, thus to mimic a more physiologically relevant 3D cellular environment. To accomplish this, we constructed perfusable microfluidic channels by embedding sacrificial circular gelatin vascular templates in collagen, which were removed through temperature control. Motile breast cancer cells were pre-seeded into the collagen matrix and when presented with a controlled chemical stimulation from the artificial vasculature, they migrated towards the vasculature structure. We believe this innovative vascular 3D ECM system can be used to provide novel insights into cellular dynamics during multidirectional chemokineses and chemotaxis that exist in cancer and other diseases.
Antibunching-like behavior of mesoscopic light
Authors: Allevi Alessia , Bondani Maria
Published: November 2017

We present the implementation of a compact setup for the generation of sub-Poissonian states of light exhibiting the analogous of antibunching behavior in the so-called mesoscopic intensity domain. In the scheme, the idler arm of a pulsed multi-mode twin-beam state is directly measured by a photon-number-resolving detector, whereas the signal arm is divided at a balanced beam splitter, at whose outputs other two photon-number-resolving detectors measure the number of photons. The three detectors measure synchronous with each laser pulse. Due to the nonclassical correlations in the twin beam, when a given value of photons is measured in the idler arm, the conditional states obtained in post processing at the two beam-splitter outputs are nonclassical, showing lower-than-one values of the Fano factor and of the photon autocorrelation coefficient. The possibility to engineer sub-Poissonian states nearly approaching the Fock state with one photon is also addressed.
Multi-physics interactions drive VEGFR2 relocation on endothelial cells
Authors: Damioli Valentina , Salvadori Alberto , Beretta Gian Paolo , Ravelli Cosetta , Mitola Stefania
Published: November 2017

Vascular Endothelial Growth Factor Receptor-2 (VEGFR2) is a pro-angiogenic receptor, expressed on endothelial cells (ECs). Although biochemical pathways that follow the VEGFR2 activation are well established, knowledge about the dynamics of receptors on the plasma membrane remains limited. Ligand stimulation induces the polarization of ECs and the relocation of VEGFR2, either in cell protrusions or in the basal aspect in cells plated on ligand-enriched extracellular matrix (ECM). We develop a mathematical model in order to simulate the relocation of VEGFR2 on the cell membrane during the mechanical adhesion of cells onto a ligand-enriched substrate. Co-designing thein vitroexperiments with the simulations allows identifying three phases of the receptor dynamics, which are controlled respectively by the high chemical reaction rate, by the mechanical deformation rate, and by the diffusion of free receptors on the membrane. The identification of the laws that regulate receptor polarization opens new perspectives toward developing innovative anti-angiogenic strategies through the modulation of EC activation.
Characterization of liposomal carriers for the trans-scleral transport of Ranibizumab
Authors: Joseph Rini Rachel , Tan Dulcia Wei Ni , Ramon Moreno Raja Miguel , Natarajan Jayaganesh V. , Agrawal Rupesh , Wong Tina T , Venkatraman Subbu S
Published: November 2017

Age-related macular degeneration (AMD) is a leading cause of blindness in the modern world. The standard treatment regimen for neovascular AMD is the monthly/bimonthly intravitreal injection of anti-VEGF agents such as ranibizumab or aflibercept. However, these repeated invasive injections can lead to sight-threatening complications. Sustained delivery by encapsulation of the drug in carriers is a way to reduce the frequency of these injections. Liposomes are biocompatible, non-toxic vesicular nanocarriers, which can be used to encapsulate therapeutic agents to provide sustained release. The protein encapsulation was performed by a modified dehydration-rehydration (DRV) method. The liposomes formed were characterized for size, zeta potential, encapsulation efficiency, stability, in vitro release, and ex vivo release profiles. In addition, the localization of the liposomes themselves was studied ex vivo. Entrapment-efficiency of ranibizumab into 100-nm liposomes varied from 14.7 to 57.0%. Negatively-charged liposomes prepared from DPPC-DPPG were found to have the slowest release with a low initial burst release compared to the rest of liposomal formulations. The ex vivo protein release was found to slower than the in vitro protein release for all samples. In conclusion, the DPPC-DPPG liposomes significantly improved the encapsulation and release profile of ranibizumab.
Fano resonances in bilayer graphene superlattices
Authors: Briones-Torres J. A. , Rodríguez-Vargas I.
Published: November 2017

In this work, we address the ubiquitous phenomenon of Fano resonances in bilayer graphene. We consider that this phenomenon is as exotic as other phenomena in graphene because it can arise without an external extended states source or elaborate nano designs. However, there are not theoretical and/or experimental studies that report the impact of Fano resonances on the transport properties. Here, we carry out a systematic assessment of the contribution of the Fano resonances on the transport properties of bilayer graphene superlattices. Specifically, we find that by changing the number of periods, adjusting the barriers height as well as modifying the barriers and wells width it is possible to identify the contribution of Fano resonances on the conductance. Particularly, the coupling of Fano resonances with the intrinsic minibands of the superlattice gives rise to specific and identifiable changes in the conductance. Moreover, by reducing the angular range for the computation of the transport properties it is possible to obtain conductance curves with line-shapes quite similar to the Fano profile and the coupling profile between Fano resonance and miniband states. In fact, these conductance features could serve as unequivocal characteristic of the existence of Fano resonances in bilayer graphene.
Altered resting-state brain activity in Parkinson’s disease patients with freezing of gait
Authors: Mi Tao-Mian , Mei Shan-Shan , Liang Pei-Peng , Gao Lin-Lin , Li Kun-Cheng , Wu Tao , Chan Piu
Published: November 2017

Freezing of gait (FOG) is a common and debilitating symptom in Parkinson’s disease (PD). The current study investigated alterations of resting-state spontaneous brain activity in PD patients with FOG. A total of 29 patients with FOG, 28 patients without FOG and 31 controls were included. All subjects underwent resting-state functional MRI, and the amplitude of low-frequency fluctuation (ALFF) was calculated to measure the spontaneous brain activity. Between-group differences and correlations with FOG severity (both subjective and objective measures) were analyzed. Compared to those without FOG, patients with FOG showed increased ALFF in right anterior cingulate cortex (ACC) and left inferior parietal lobule (IPL), as well as decreased ALFF in right superior frontal gyrus (SFG), bilateral cerebellum and left thalamus. Correlation analyses demonstrated that ALFF within the right SFG, right ACC and bilateral pallidum were positively correlated with FOG; while ALFF within the thalamus, putamen, cerebellum and sensorimotor regions were negatively correlated. Our results indicate that FOG is associated with dysfunction within frontal-parietal regions, along with increased inhibitory outputs from basal ganglia. Additionally, altered activity of cerebellum implicates its role in the pathophysiology of FOG. These findings provide further insight into the underlying neural mechanisms of FOG in PD patients.
The role of the systemic inflammatory response in predicting outcomes in patients with operable cancer: Systematic review and meta-analysis
Authors: Dolan Ross D. , Lim Jason , McSorley Stephen T. , Horgan Paul G. , McMillan Donald C.
Published: November 2017

Cancer remains a leading causes of death worldwide and an elevated systemic inflammatory response (SIR) is associated with reduced survival in patients with operable cancer. This review aims to examine the evidence for the role of systemic inflammation based prognostic scores in patients with operable cancers. A wide-ranging literature review using targeted medical subject headings for human studies in English was carried out in the MEDLINE, EMBASE, and CDSR databases until the end of 2016. The SIR has independent prognostic value, across tumour types and geographical locations. In particular neutrophil lymphocyte ratio (NLR) (n = 158), platelet lymphocyte ratio (PLR) (n = 68), lymphocyte monocyte ratio (LMR) (n = 21) and Glasgow Prognostic Score/ modified Glasgow Prognostic Score (GPS/mGPS) (n = 60) were consistently validated. On meta-analysis there was a significant relationship between elevated NLR and overall survival (OS) (p < 0.00001)/ cancer specific survival (CSS) (p < 0.00001), between elevated LMR and OS (p < 0.00001)/CSS (p < 0.00001), and elevated PLR and OS (p < 0.00001)/CSS (p = 0.005). There was also a significant relationship between elevated GPS/mGPS and OS (p < 0.00001)/CSS (p < 0.00001). These results consolidate the prognostic value of the NLR, PLR, LMR and GPS/mGPS in patients with resectable cancers. This is particularly true for the NLR/GPS/mGPS which should form part of the routine preoperative and postoperative workup.
The repeat region of cortactin is intrinsically disordered in solution
Authors: Li Xiaofeng , Tao Yeqing , Murphy James W. , Scherer Alexander N. , Lam TuKiet T. , Marshall Alan G. , Koleske Anthony J. , Boggon Titus J.
Published: November 2017

The multi-domain protein, cortactin, contains a 37-residue repeating motif that binds to actin filaments. This cortactin repeat region comprises 6½ similar copies of the motif and binds actin filaments. To better understand this region of cortactin, and its fold, we conducted extensive biophysical analysis. Size exclusion chromatography with multi-angle light scattering (SEC-MALS) reveals that neither constructs of the cortactin repeats alone or together with the adjacent helical region homo-oligomerize. Using circular dichroism (CD) we find that in solution the cortactin repeats resemble a coil-like intrinsically disordered protein. Small-angle X-ray scattering (SAXS) also indicates that the cortactin repeats are intrinsically unfolded, and the experimentally observed radius of gyration (Rg) is coincidental to that calculated by the program Flexible-Meccano for an unfolded peptide of this length. Finally, hydrogen-deuterium exchange mass spectrometry (HDX-MS) indicates that the domain contains limited hydrophobic core regions. These experiments therefore provide evidence that in solution the cortactin repeat region of cortactin is intrinsically disordered.
Efficiency of novel nanocombinations of bovine milk proteins (lactoperoxidase and lactoferrin) for combating different human cancer cell lines
Authors: Abu-Serie Marwa M. , El-Fakharany Esmail M.
Published: November 2017

Bovine lactoperoxidase (LPO) and lactoferrin (LF) are suitable proteins to be loaded or adsorbed to chitosan nanoparticles (NPs) for preparing stable nanoformulations with potent anticancer activity. In the present study, nanocombinations of LPO and LF revealed improvement in their stability and activity compared to single (free or nanoformulated) bovine proteins. The coating or loading of LPO-loaded NPs with LF resulted in the highest synergistic cytotoxicity effect against Caco-2, HepG-2, MCF-7 and PC-3 cells in comparison with other NPs and free proteins without causing toxicity toward normal cells. This synergistic improvement in the anticancer activity was apoptosis-dependent that was confirmed by severe alterations in cellular morphology, high percentage of annexin-stained cells and sub-G1 populations as well as nuclear staining with orange fluorescence of treated cancer cells. Additionally, significant alterations in the expression of well characterized cellular proliferation and apoptosis guards (NF-κB, Bcl-2 and p53) in these NPs-treated cancer cells compared to 5-fluorouracil (5-FU) treated cells. Our findings provide for the first time that these new synergistic nanoformulated forms of LPO and LF were superior in their selective apoptosis-mediating anticancer effect than free form of these proteins and 5-FU. LF coating or loading of LPO-loaded NPs present as promising therapy for cancer.
Influence of the electrolyte’s pH on the properties of electrochemically deposited hydroxyapatite coating on additively manufactured Ti64 alloy
Authors: Vladescu Alina , Vranceanu Diana M. , Kulesza Slawek , Ivanov Alexey N. , Bramowicz Mirosław , Fedonnikov Alexander S. , Braic Mariana , Norkin Igor A. , Koptyug Andrey , Kurtukova Maria O. , Dinu Mihaela , Pana Iulian , Surmeneva Maria A. , Surmenev Roman A. , Cotrut Cosmin M.
Published: November 2017

Properties of the hydroxyapatite obtained by electrochemical assisted deposition (ED) are dependent on several factors including deposition temperature, electrolyte pH and concentrations, applied potential. All of these factors directly influence the morphology, stoichiometry, crystallinity, electrochemical behaviour, and particularly the coating thickness. Coating structure together with surface micro- and nano-scale topography significantly influence early stages of the implant bio-integration. The aim of this study is to analyse the effect of pH modification on the morphology, corrosion behaviour and in vitro bioactivity and in vivo biocompatibility of hydroxyapatite prepared by ED on the additively manufactured Ti64 samples. The coatings prepared in the electrolytes with pH = 6 have predominantly needle like morphology with the dimensions in the nanometric scale (~30 nm). Samples coated at pH = 6 demonstrated higher protection efficiency against the corrosive attack as compared to the ones coated at pH = 5 (~93% against 89%). The in vitro bioactivity results indicated that both coatings have a greater capacity of biomineralization, compared to the uncoated Ti64. Somehow, the coating deposited at pH = 6 exhibited good corrosion behaviour and high biomineralization ability. In vivo subcutaneous implantation of the coated samples into the white rats for up to 21 days with following histological studies showed no serious inflammatory process.
Mutational analysis of TSC1 and TSC2 genes in Tuberous Sclerosis Complex patients from Greece
Authors: Avgeris Socratis , Fostira Florentia , Vagena Andromachi , Ninios Yiannis , Delimitsou Angeliki , Vodicka Radek , Vrtel Radek , Youroukos Sotirios , Stravopodis Dimitrios J. , Vlassi Metaxia , Astrinidis Aristotelis , Yannoukakos Drakoulis , Voutsinas Gerassimos E.
Published: November 2017

Tuberous sclerosis complex (TSC) is a rare autosomal dominant disorder causing benign tumors in the brain and other vital organs. The genes implicated in disease development are TSC1 and TSC2. Here, we have performed mutational analysis followed by a genotype-phenotype correlation study based on the clinical characteristics of the affected individuals. Twenty unrelated probands or families from Greece have been analyzed, of whom 13 had definite TSC, whereas another 7 had a possible TSC diagnosis. Using direct sequencing, we have identified pathogenic mutations in 13 patients/families (6 in TSC1 and 7 in TSC2), 5 of which were novel. The mutation identification rate for patients with definite TSC was 85%, but only 29% for the ones with a possible TSC diagnosis. Multiplex ligation-dependent probe amplification (MLPA) did not reveal any genomic rearrangements in TSC1 and TSC2 in the samples with no mutations identified. In general, TSC2 disease was more severe than TSC1, with more subependymal giant cell astrocytomas and angiomyolipomas, higher incidence of pharmacoresistant epileptic seizures, and more severe neuropsychiatric disorders. To our knowledge, this is the first comprehensive TSC1 and TSC2 mutational analysis carried out in TSC patients in Greece.
Mitotic slippage and the subsequent cell fates after inhibition of Aurora B during tubulin-binding agent–induced mitotic arrest
Authors: Tsuda Yasuo , Iimori Makoto , Nakashima Yuichiro , Nakanishi Ryota , Ando Koji , Ohgaki Kippei , Kitao Hiroyuki , Saeki Hiroshi , Oki Eiji , Maehara Yoshihiko
Published: November 2017

Tubulin-binding agents (TBAs) are designed to target microtubule (MT) dynamics, resulting in compromised mitotic spindles and an unsatisfied spindle assembly checkpoint. The activity of Aurora B kinase is indispensable for TBA-induced mitotic arrest, and its inhibition causes mitotic slippage and postmitotic endoreduplication. However, the precise phenomenon underlying mitotic slippage, which is caused by treatment with both Aurora B inhibitors and TBAs, and the cell fate after postmitotic slippage are not completely understood. Here, we found that HeLa and breast cancer cells treated with the different types of TBAs, such as paclitaxel and eribulin (MT-stabilizing and MT-destabilizing agents, respectively), exhibited distinct behaviors of mitotic slippage on inhibition of Aurora B. In such conditions, the cell fates after postmitotic slippage vastly differed with respect to cell morphology, cell proliferation, and cytotoxicity in short-term culture; that is, the effects of inhibition of Aurora B were beneficial for cytotoxicity enhancement in eribulin treatment but not in paclitaxel. However, in long-term culture, the cells that survived after mitotic slippage underwent endoreduplication and became giant cells in both cases, resulting in cellular senescence. We propose that MT-destabilizing agents may be more appropriate than MT-stabilizing agents for treating cancer cells with a weakened Aurora B kinase activity.
Physics of Efficiency Droop in GaN:Eu Light-Emitting Diodes
Authors: Fragkos Ioannis E. , Dierolf Volkmar , Fujiwara Yasufumi , Tansu Nelson
Published: November 2017

The internal quantum efficiency (IQE) of an electrically-driven GaN:Eu based device for red light emission is analyzed in the framework of a current injection efficiency model (CIE). The excitation path of the Eu+3 ion is decomposed in a multiple level system, which includes the carrier transport phenomena across the GaN/GaN:Eu/GaN active region of the device, and the interactions among traps, Eu+3 ions and the GaN host. The identification and analysis of the limiting factors of the IQE are accomplished through the CIE model. The CIE model provides a guidance for high IQE in the electrically-driven GaN:Eu based red light emitters.
Bayesian Analysis of MicroScale Thermophoresis Data to Quantify Affinity of Protein:Protein Interactions with Human Survivin
Authors: Garcia-Bonete Maria-Jose , Jensen Maja , Recktenwald Christian V. , Rocha Sandra , Stadler Volker , Bokarewa Maria , Katona Gergely
Published: November 2017

A biomolecular ensemble exhibits different responses to a temperature gradient depending on its diffusion properties. MicroScale Thermophoresis technique exploits this effect and is becoming a popular technique for analyzing interactions of biomolecules in solution. When comparing affinities of related compounds, the reliability of the determined thermodynamic parameters often comes into question. The thermophoresis binding curves can be assessed by Bayesian inference, which provides a probability distribution for the dissociation constant of the interacting partners. By applying Bayesian machine learning principles, binding curves can be autonomously analyzed without manual intervention and without introducing subjective bias by outlier rejection. We demonstrate the Bayesian inference protocol on the known survivin:borealin interaction and on the putative protein-protein interactions between human survivin and two members of the human Shugoshin-like family (hSgol1 and hSgol2). These interactions were identified in a protein microarray binding assay against survivin and confirmed by MicroScale Thermophoresis.
Cell-Free DNA Kinetics in a Pre-Clinical Model of Head and Neck Cancer
Authors: Muhanna Nidal , Di Grappa Marco A. , Chan Harley H. L. , Khan Tahsin , Jin Cheng S. , Zheng Yangqiao , Irish Jonathan C. , Bratman Scott V.
Published: November 2017

In cancer patients, circulating tumour-derived DNA (ctDNA) levels imperfectly reflect disease burden apparent on medical imaging. Further evaluation of ctDNA levels over time is needed to better understand the correlation with tumour growth and therapeutic response. We describe ctDNA kinetics within an orthotopic, immunocompetent preclinical rabbit model of local-regionally advanced head and neck squamous cell carcinoma (HNSCC). Monitoring primary tumour and metastatic lymph node volume by computed tomography (CT), we observed a correlation between ctDNA levels and tumour burden. We found that ctDNA detection could precede evidence of tumour on CT. Sensitivity and specificity of ctDNA detection in this model was 90.2% (95% C.I.: 76.9–97.3%) and 85.7% (95% C.I.: 67.3–96.0%), respectively. Rapid tumour growth followed by auto-necrosis and tumour volume contraction produced a spike in ctDNA levels, suggesting that viable tumour cells may be required for sustained ctDNA release. Following surgical resection, both ctDNA and total plasma DNA were correlated with recurrent tumour volume. Our results reveal the complex kinetic behaviour of ctDNA and total plasma DNA upon tumour growth or surgery. This pre-clinical model could be useful for future studies focused on elucidating mechanisms of ctDNA release into the circulation from primary and metastatic sites.
Spontaneous Phase Transfer-Mediated Selective Removal of Heavy Metal Ions Using Biocompatible Oleic Acid
Authors: Chang Jeehan , Yoo Sooyeon , Lee Wooju , Kim Dongchoul , Kang Taewook
Published: November 2017

Here, we propose an environmentally benign removal technique for heavy metal ions based on selective and spontaneous transfer to oleic acid. The ions can be removed via (1) the selective and rapid complexation with the carboxylic end of oleic acid at an oleic acid/water interface, and (2) the diffusion of such complex into the oleic acid layer. A wide variety of heavy metal ions such as Cu2+, Pb2+, Zn2+, and Ni2+ can be selectively removed over K+ and Na+. For example, the concentration of Cu2+ is reduced to below 1.3 ppm within 24 h, which corresponds to the level of Cu2+ permitted by the Environmental Protection Agency. The addition of ethylenediamine ligand to the metal ion solutions is also shown to enhance the phase transfer. The removal efficiency is increased by up to 6 times when compared with that in the absence of the ligand and follows the order, Cu2+ (99%) > Pb2+ (96%) > Zn2+ (95%) > Ni2+ (65%). Moreover, the removal time can be shortened from 24 h to 1 h. The effect of an emulsion induced by a mechanical agitation on the removal of heavy metal ion is also studied.