bluuebunny/crossref_metadata_2025 huggingface dataset:
Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down‐regulation of HIF1 and PDK
Authors: Rodrigues A. F. , Guerreiro M. R. , Formas‐Oliveira A. S. , Fernandes P. , Blechert A.‐K. , Genzel Y. , Alves P. M. , Hu W. S. , Coroadinha A. S.
Published: July 2015

Many mammalian cell lines used in the manufacturing of biopharmaceuticals exhibit high glycolytic flux predominantly channeled to the production of lactate. The accumulation of lactate in culture reduces cell viability and may also decrease product quality. In this work, we engineered a HEK 293 derived cell line producing a recombinant gene therapy retroviral vector, by down‐regulating hypoxia inducible factor 1 (HIF1) and pyruvate dehydrogenase kinase (PDK). Specific productivity of infectious viral titers could be increased more than 20‐fold for single gene knock‐down (HIF1 or PDK) and more than 30‐fold under combined down‐regulation. Lactate production was reduced up to 4‐fold. However, the reduction in lactate production, alone, was not sufficient to enhance the titer: high‐titer clones also showed significant enrolment of metabolic routes not related to lactate production. Transcriptome analysis indicated activation of biological amines metabolism, detoxification routes, including glutathione metabolism, pentose phosphate pathway, glycogen biosynthesis and amino acid catabolism. The latter were validated by enzyme activity assays and metabolite profiling, respectively. High‐titer clones also presented substantially increased transcript levels of the viral genes expression cassettes. The results herein presented demonstrate the impact of HIF1 and PDK down‐regulation on the production performance of a mammalian cell line, reporting one of the highest fold‐increase in specific productivity of infectious virus titers achieved by metabolic engineering. They additionally highlight the contribution of secondary pathways, beyond those related to lactate production, that can be also explored to pursue improved metabolic status favoring a high‐producing phenotype. Biotechnol. Bioeng. 2016;113: 150–162. © 2015 Wiley Periodicals, Inc.
Assessment of the anatomical variation to be found in the normal tricuspid valve
Authors: Tretter Justin T. , Sarwark Anne E. , Anderson Robert H. , Spicer Diane E.
Published: July 2015

The normal tricuspid valve is tri‐leaflet, supported by tendinous cords, which are themselves supported by papillary muscles. There can be marked variation in the anatomy of the normal tricuspid valve, which must be understood to differentiate it from pathological malformations. The tricuspid valve of 100 normal heart specimens was examined. The three leaflets of the tricuspid valve, along with the papillary muscles supporting the zones of apposition were identified, and details of the anatomy recorded and analyzed. All three leaflets were identified in all 100 hearts. The septal leaflet had tendinous cord attachments in 93 specimens to the ventricular septum. The medial papillary muscle had a single head in the majority of specimens, supporting the zone of apposition with the antero‐superior leaflet in 97 specimens. The anterior papillary muscle attached to the mid‐portion of the antero‐superior leaflet in 62 specimens, and supplied the zone of apposition between the antero‐superior and inferior leaflets in 81 specimens. There were rough zone cord attachments to the antero‐superior leaflets in all specimens. The inferior leaflet had basal cord attachments in 87 specimens, with attachments to multiple small muscular heads in 37 specimens. The inferior papillary muscle was well formed in only 58 specimens. Although certain features are relatively constant, multiple variations in the normal tricuspid valve have been identified. Knowledge of these normal variations is necessary in understanding the function of this complex valve apparatus, along with the potential for pathology. Clin. Anat. 29:399–407, 2016. © 2015 Wiley Periodicals, Inc.
Demographic patterns of cutaneous T‐cell lymphoma incidence in Texas based on two different cancer registries
Authors: Litvinov Ivan V. , Tetzlaff Michael T. , Rahme Elham , Jennings Michelle A. , Risser David R. , Gangar Pamela , Netchiporouk Elena , Moreau Linda , Prieto Victor G. , Sasseville Denis , Duvic Madeleine
Published: July 2015

Cutaneous T‐cell lymohomas (CTCLs) are rare, but potentially devastating malignancies, with Mycosis fungoides and Sézary Syndrome being the most common. In our previous study, we identified and described regions of geographic clustering of CTCL cases in Texas by analyzing ~1990 patients using two distinct cancer registries. In the current work, we describe in detail demographic patterns for this malignancy in our study population and apply logistic regression models to analyze the incidence of CTCL by sex, race, age, and clinical stage at the time of diagnosis. Furthermore, using Fisher's exact test, we analyze changes in incidence over time in the identified Houston communities with unusually high CTCL incidence. While CTCL primarily affects Caucasian individuals >55 years old, we confirm that it presents at a younger age and with more advanced disease stages in African‐American and Hispanic individuals. Also, we demonstrate a significant increase in CTCL incidence over time in the identified communities. Spring, Katy, and Houston Memorial areas had high baseline rates. Furthermore, a statistically significant disease surge was observed in these areas after ~2005. This report supplements our initial study documenting the existence of geographic clustering of CTCL cases in Texas and in greater detail describes demographic trends for our patient population. The observed surge in CTCL incidence in the three identified communities further argues that this malignancy may be triggered by one or more external etiologic agents.
CuI and H2O2 Inactivate and FeII Inhibits [Fe]‐Hydrogenase at Very Low Concentrations
Authors: Hidese Ryota , Ataka Kenichi , Bill Eckhard , Shima Seigo
Published: July 2015

[Fe]‐Hydrogenase (Hmd) catalyzes reversible hydride transfer from H2. It harbors an iron‐guanylylpyridinol as a cofactor with an FeII that is ligated to one thiolate, two COs, one acyl‐C, one pyridinol‐N, and solvent. Here, we report that CuI and H2O2 inactivate Hmd (half‐maximal rates at 1 μM CuI and 20 μM H2O2) and that FeII inhibits the enzyme with very high affinity (Ki=40 nM). Infrared and EPR studies together with competitive inhibition studies with isocyanide indicated that CuI exerts its inhibitory effect most probably by binding to the active site iron‐thiolate ligand. Using the same methods, it was found that H2O2 binds to the active‐site iron at the solvent‐binding site and oxidizes FeII to FeIII. Also it was shown that FeII reversibly binds away from the active site iron, with binding being competitive to the organic hydride acceptor; this inhibition is specific for FeII and is reminiscent of that for the [FeFe]‐hydrogenase second iron, which specifically interacts with H2.
ChemInform Abstract: Sunlight‐Activated Eu2+/Dy3+ Doped SrAl2O4 Water Resistant Phosphorescent Layer for Optical Displays and Defence Applications.
Authors: Kumar Arun , Kedawat Garima , Kumar Pawan , Dwivedi Jaya , Gupta Bipin Kumar
Published: July 2015

A long persistent, transparent, flexible, and water resistant phosphorescent layer of sunlight‐activated green luminescent Sr1‐x‐yAl2O4:Eux 2+/Dyy3+ (x = 0.05—0.3, y = 0.1—0.6) is fabricated via solid state reaction/solution casting of SrCO3, Al2O3, Eu2O3, and Dy2O3 (10% H2/N2 atmosphere, alumina crucible, 1300 °C, 3 h).
ChemInform Abstract: Enhanced Thermoelectric Performance of N‐Type Bi2Se3 Doped with Cu.
Authors: Sun Guolong , Qin Xiaoying , Li Di , Zhang Jian , Ren Baojin , Zou Tianhua , Xin Hongxing , Paschen Silke Buehler , Yan Xinlin
Published: July 2015

Single phase samples of CuxBi2Se3 (x = 0‐0.020) are prepared by melting and vacuum hot pressing stoichiometric amounts of the elements (563 K, 300 MPa, 60 min).
ChemInform Abstract: Synthesis, Crystal Structure and Physical Properties of [Li0.85Fe0.15OH][FeS].
Authors: Zhang Xian , Lai Xiaofang , Yi Na , He Jianqiao , Chen Haijie , Zhang Hui , Lin Jianhua , Huang Fuqiang
Published: July 2015

Phase‐pure [Li0.85Fe0.15OH][FeS] is prepared by hydrothermal reaction of Fe, (NH2)2CS, and LiOH (autoclave, 200 °C, 3 d).
ChemInform Abstract: Rh(II)‐Catalyzed Cycloadditions of 1‐Tosyl 1,2,3‐Triazoles with 2H‐Azirines: Switchable Reactivity of Rh‐Azavinylcarbene as [2C]‐ or Aza‐[3C]‐Synthon.
Authors: Wang Yuanhao , Lei Xiaoqiang , Tang Yefeng
Published: July 2015

In some cases it is possible to divert the outcome of the reaction in favor of aminopyrroles and dihydropyrazines, respectively by altering the reaction conditions [e.g. reaction of (XII) and (XV)].
ChemInform Abstract: Intramolecular Aminochalcogenation and Diamination of Alkenes Employing N‐Iodosuccinimide.
Authors: Zhang Jun , Zhang Xuejun , Wu Weijie , Zhang Gengtao , Xu Sheng , Shi Min
Published: July 2015

A new NIS‐mediated intramolecular diamination and aminosulfuration of alkenes with N‐alkyl or N‐aryl thioureas is presented.
ChemInform Abstract: A Strategy for Generating Alkyl Radicals from Aliphatic Esters and Lactones via Sequential Hydrolysis and Photoinduced Decarboxylation.
Authors: Saito Hikaru , Kanetake Takayuki , Osaka Kazuyuki , Maeda Kousuke , Morita Toshio , Yoshimi Yasuharu
Published: July 2015

Alkyl radicals generated from substrates (I) and (IX) react with acrylonitrile, tert‐dodecanethiol, or 1,4‐dicyanobenzene to afford the corresponding addition, reduction, and substitution products.
ChemInform Abstract: Zinc‐Catalyzed Selective Reduction of Cyclic Imides with Hydrosilanes: Synthesis of ω‐Hydroxylactams.
Authors: Ding Guangni , Lu Bin , Li Yuyuan , Wan Jun , Zhang Zhaoguo , Xie Xiaomin
Published: July 2015

The hydrosilylation shows remarkable regioselectivity for unsymmetrical imides bearing coordinating groups adjacent to the carbonyl.
ChemInform Abstract: Iodobenzene Dichloride Mediated Sequential C—Cl Bond Formation: A Safe, Convenient and Efficient Method for the Direct α,α‐Dichlorination of β‐Dicarbonyl Compounds.
Authors: Duan Xiyan , Zhou Huiyun , Tian Xinlei , Liu Jianwei , Ma Junying
Published: July 2015

Whereas the α,α‐dichlorination of β‐dicarbonyl compounds proceeds efficiently, it is not applicable to the corresponding transformation of simple ketones.
ChemInform Abstract: Catalyst‐Free and Metal‐Free Electrophilic Bromoamidation of Unactivated Olefins Using the N‐Bromosuccinimide/Sulfonamide Protocol.
Authors: Yu Wesley Zongrong , Chen Feng , Cheng Yi An , Yeung Ying‐Yeung
Published: July 2015

An efficient and metal catalyst‐free protocol is used for the synthesis of brominated sulfonamides from aromatic, aliphatic, and cycloaliphatic olefins.
ChemInform Abstract: Kinetic Resolution in the [2 + 2] Cycloaddition of Ketenes: An Experimental and Theoretical Study.
Authors: Rulliere Pauline , Carret Sebastien , Milet Anne , Poisson Jean‐Francois
Published: July 2015

The procedure uses the high difference in reactivity and thus allows the access to pure (E) alkenes, often difficult or impossible to isolate from the mixtures or expensive in trade.
ChemInform Abstract: One Pot Domino Reaction Accessing γ‐Nitroesters: Synthesis of GABA Derivatives.
Authors: Naciuk Fabricio F. , Vargas Debora Z. , D'Oca Caroline R. M. , Moro Celso C. , Russowsky Dennis
Published: July 2015

Various γ‐nitroesters are efficiently synthesized by Michael addition of 1,3‐dicarbonyl compounds to nitrostyrenes using Mg‐Al‐hydrotalcite as catalyst.
ChemInform Abstract: Additive‐Free Decarboxylative Coupling of Cinnamic Acid Derivatives in Water: Synthesis of Allyl Amines.
Authors: Park Kyungho , Lee Sunwoo
Published: July 2015

This is the first example of an additive‐free decarboxylative coupling of cinnamic acid derivatives with formaldehyde and amines to give the corresponding allyl amines.
ChemInform Abstract: Organoselenium‐Catalyzed Baeyer—Villiger Oxidation of α,β‐Unsaturated Ketones by Hydrogen Peroxide to Access Vinyl Esters.
Authors: Zhang Xu , Ye Jianqing , Yu Lei , Shi Xinkang , Zhang Ming , Xu Qing , Lautens Mark
Published: July 2015

A practical and functional group‐tolerant chemoselective preparation of various (E)‐vinyl esters from readily available (E)‐α,β‐unsaturated ketones is described.
ChemInform Abstract: Synthesis of Sulfonamides via I2‐Mediated Reaction of Sodium Sulfinates with Amines in an Aqueous Medium at Room Temperature.
Authors: Pan Xiaojun , Gao Jian , Liu Juan , Lai Junyi , Jiang Huanfeng , Yuan Gaoqing
Published: July 2015

Compared with previous studies, the introduced procedure has the advantages of simplicity, environmentally friendliness, and low costs.
ChemInform Abstract: Platinum‐Catalyzed Asymmetric Ring‐Opening Reactions of Oxabenzonorbornadienes with Phenols.
Authors: Meng Ling , Yang Wen , Pan Xuejing , Tao Meng , Cheng Guo , Wang Sanyong , Zeng Heping , Long Yuhua , Yang Dingqiao
Published: July 2015

Optimized conditions A) are elaborated in detail.
ChemInform Abstract: Rhodium‐Catalyzed Dehydrogenative Coupling of Phenylheteroarenes with Alkynes or Alkenes.
Authors: Iitsuka Tomonori , Hirano Koji , Satoh Tetsuya , Miura Masahiro
Published: July 2015

The Rh‐catalyzed alkenylation of 3‐phenylthiophene (I) with alkenes (II) affords (E)‐ortho‐products (IIIa‐g).
ChemInform Abstract: Mild Microwave‐Assisted Synthesis of Dipyrromethanes and Their Analogues.
Authors: Xiong Ruisheng , Borbas K. Eszter
Published: July 2015

The Mannich reaction between pyrroles or indoles and Eschenmoser′s salt (II) forms N‐aminomethylated derivatives with high regioselectivity, which undergo substitution with pyrrole (III) under microwave irradiation, affording the corresponding dipyrromethanes such as (IV) and (VI) and their analogues (VIII).
ChemInform Abstract: RhII‐Catalyzed [3 + 2] Cycloaddition of 2H‐Azirines with N‐Sulfonyl‐1,2,3‐triazoles.
Authors: Zhao Yun‐Zhou , Yang Hai‐Bin , Tang Xiang‐Ying , Shi Min
Published: July 2015

The title reaction affords a series of fully functionalized pyrrole derivatives (III) and (V) via rhodium azavinyl carbene intermediates.
ChemInform Abstract: Visible Light Induced Radical Cyclization of o‐Iodophenylacrylamides: A Concise Synthesis of Indolin‐2‐one.
Authors: Dong Wuheng , Liu Yan , Hu Bei , Ren Kai , Li Yuyuan , Xie Xiaomin , Jiang Yuexiu , Zhang Zhaoguo
Published: July 2015

The reaction proceeds through 5‐exo‐trig radical cyclization.
ChemInform Abstract: Palladium Catalyzed Asymmetric Allylation of 3‐OBoc‐Oxindoles: An Efficient Synthesis of 3‐Allyl‐3‐hydroxyoxindoles.
Authors: Jayakumar Samydurai , Kumarswamyreddy Nandarapu , Prakash Muthuraj , Kesavan Venkitasamy
Published: July 2015

A tartrate derived bi(oxazoline) ligand causes remarkable asymmetric induction in the Pd‐catalyzed allylation of protected oxindoles.
ChemInform Abstract: Tuning of Tetrathiafulvalene Properties: Versatile Synthesis of N‐Arylated Monopyrrolotetrathiafulvalenes via Ullmann‐Type Coupling Reactions.
Authors: Azov Vladimir A , Janott Diana , Schlueter Dirk , Zeller Matthias
Published: July 2015

N‐Arylation of pyrrolotetrathiafulvalenes by aryl halides gives the corresponding Ullmann‐type products, which were studied by UV spectroscopy, electrochemistry, and crystallography.
ChemInform Abstract: Rhodium/Chiral Diene Complexes in the Catalytic Asymmetric Arylation of β‐Pyrazol‐1‐yl Acrylates.
Authors: Gopula Balraj , Tsai Yun‐Fan , Kuo Ting‐Shen , Wu Ping‐Yu , Henschke Julian P. , Wu Hsyueh‐Liang
Published: July 2015

The reaction proceeds with high regio‐ and enantioselectivity and can be applied in the formal synthesis of the bioactive compound (XIII), an agonist toward the human GPR40 G‐protein‐coupled receptor.
ChemInform Abstract: Metal‐Free Synthesis of 3,5‐Disubstituted 1H‐ and 1‐Aryl‐1H‐pyrazoles from 1,3‐Diyne‐indole Derivatives Employing Two Successive Hydroaminations.
Authors: Bassaco Mariana M. , Fortes Margiani P. , Kaufman Teodoro S. , Silveira Claudio C.
Published: July 2015

Under optimized and mild reaction conditions, symmetric and non‐symmetric 1,3‐diyne‐indole derivatives react with hydrazine and a variety of arylhydrazines.
ChemInform Abstract: Efficient Domino Synthesis of Benzimidazole Derivatives: Copper Catalysis versus Transition Metal‐Free Conditions.
Authors: Liu Haiquan , Tang Jiaming , Jiang Liu , Zheng Tingting , Wang Xiaoxia , Lv Xin
Published: July 2015

Domino reactions of o‐haloaryl carbodiimides with active methylene compounds allow the synthesis of a wide variety of 2‐alkyl and 2‐alkenyl benzimidazole derivatives.
ChemInform Abstract: A Novel Three‐Component Reaction Involving Terminal Alkynes, Elemental Sulfur, and Strained Heterocycles.
Authors: Samzadeh‐Kermani Alireza
Published: July 2015

as a facile route for the synthesis of functionalized 1,3‐oxathiolanes (III) and 1,3‐thiazolidines (V)
ChemInform Abstract: SiO2—H3BO3 Promoted Solvent‐Free, Green and Sustainable Synthesis of Bioactive 1‐Substituted‐1H‐tetrazole Analogues.
Authors: Parveen Mehtab , Ahmad Faheem , Mohammed Malla Ali , Azaz Shaista
Published: July 2015

Multi‐functionalized title compounds (III) are synthesized by condensation of various heterocyclic/aromatic primary amines (I), sodium azide, and triethyl orthoformate (II) without use of an environmentally toxic solvent.
ChemInform Abstract: Discovery of Antibiotic (E)‐3‐(3‐Carboxyphenyl)‐2‐(4‐cyanostyryl)quinazolin‐4(3H)‐one.
Authors: Chang Mayland , et al.
Published: July 2015

A library of quinazolinones is prepared with a view to developing novel classes of antibiotics.
ChemInform Abstract: An Efficient Approach to gem‐Difluorocyclopropylstannanes via Highly Regio‐ and Stereoselective Hydrostannylation of gem‐Difluorocyclopropenes and Their Unique Ring‐Opening Reaction to Afford β‐Fluoroallylic Alcohols.
Authors: Nihei T. , Hoshino T. , Konno T.
Published: July 2015

Compound (VIg) undergoes ring‐opening reactions with water, alcohols, carboxylic acids and amides as nucleophiles.
ChemInform Abstract: Chemical Synthesis of the Tumor‐Associated Globo H Antigen.
Authors: Mandal Satadru S. , Liao Guochao , Guo Zhongwu
Published: July 2015

A derivative of the tumor‐associated globo H antigen, a complex hexasaccharide, is synthesized by convergent and efficient [3+2+1] strategy using various glycosylation methods.
ChemInform Abstract: New Duclauxamide from Penicillium manginii YIM PH30375 and Structure Revision of the Duclauxin Family.
Authors: Cao Pei , Yang Jing , Miao Cui‐Ping , Yan Yijun , Ma Ya‐Tuan , Li Xiao‐Nian , Zhao Li‐Xing , Huang Sheng‐Xiong
Published: July 2015

The structural eludication of the title compound (I) confirms the C‐9′ configuration to be (S) and therefore also revises the structures of other duclauxin analogues.
ChemInform Abstract: Hypersubones A and B, New Polycyclic Acylphloroglucinols with Intriguing Adamantane Type Cores from Hypericum subsessile.
Authors: Liao Yang , Liu Xia , Yang Jing , Lao Yuan‐Zhi , Yang Xing‐Wei , Li Xiao‐Nian , Zhang Jing‐Jing , Ding Zhi‐jie , Xu Hong‐Xi , Xu Gang
Published: July 2015

Hypersubones A (II) and B (II) are tested on their cytotoxicity against 4 human cancer cell lines: the HepG2 human hepatocarcinoma cell line, the Eca109 esophageal carcinoma cell line, the HeLa cervical cancer cell line and the A549 non‐small cell lung cancer cell line.
ChemInform Abstract: Noble Metal Alloy Complex Nanostructures: Controllable Synthesis and Their Electrochemical Property
Authors: Liu Hui‐ling , Nosheen Farhat , Wang Xun
Published: July 2015

Review: 157 refs.
ChemInform Abstract: Atomic‐Scale Derivatives of Solid‐State Materials
Authors: Li Tianyang , Goldberger Joshua E.
Published: July 2015

Review: synthesis, properties, applications; 113 refs.
ChemInform Abstract: Properties and Applications of Flavonoid Metal Complexes
Authors: Kasprzak Maria M. , Erxleben Andrea , Ochocki Justyn
Published: July 2015

Review: 334 refs.
ChemInform Abstract: Ionic Liquids and Continuous Flow Processes: A Good Marriage to Design Sustainable Processes
Authors: Garcia‐Verdugo Eduardo , Altava Belen , Burguete M. Isabel , Lozano Pedro , Luis S. V.
Published: July 2015

Review: [111 refs.
ChemInform Abstract: Recent Advances in the N‐Heterocyclic Carbene (NHC)‐Organocatalyzed Stetter Reaction and Related Chemistry
Authors: Yetra Santhivardhana Reddy , Patra Atanu , Biju Akkattu T.
Published: July 2015

Review: [94 refs.
ChemInform Abstract: Supramolecular Catalysis in Metal‐Ligand Cluster Hosts
Authors: Brown Casey J. , Toste F. Dean , Bergman Robert G. , Raymond Kenneth N.
Published: July 2015

Review: 124 refs.
ChemInform Abstract: An Overview of Self‐Assembly and Morphological Regulation of Amphiphilic DNA Organic Hybrids
Authors: Wu Fen , Zhang Yiyang , Yang Zhongqiang
Published: July 2015

Review: 74 refs.
ChemInform Abstract: Multiple Roles of Graphene in Heterogeneous Catalysis
Authors: Fan Xiaobin , Zhang Guoliang , Zhang Fengbao
Published: July 2015

Review: 141 refs.
ChemInform Abstract: Handling Diazonium Salts in Flow for Organic and Material Chemistry
Authors: Oger Nicolas , Le Grognec Erwan , Felpin Francois‐Xavier
Published: July 2015

Review: 105 refs.
ChemInform Abstract: Synthesis of Green Fuels from Biogenic Waste Through Thermochemical Route — The Role of Heterogeneous Catalyst: A Review
Authors: Mohanty Pravakar , Pant Kamal K. , Naik Satya Narayan , Parikh Jigisha , Hornung Andreas , Sahu J. N.
Published: July 2015

Review: 159 refs.
ChemInform Abstract: Polymer Electrolyte for Photoelectrochemical Cell and Dye‐Sensitized Solar Cell: A Brief Review
Authors: Rahman M. Y. A. , Ahmad A. , Umar A. A. , Taslim R. , Su'ait M. S. , Salleh M. M.
Published: July 2015

Review: 45 refs.
Estrogenic Activity Assessment in Environmental Matrices by Means of In Vitro Cell‐Based Bioassays Coupled With Molecular Investigation
Authors: Etteieb Selma , Kawachi Atsushi , Han Junkyu , Tarhouni Jamila , Isoda Hiroko
Published: July 2015

Pollution of river systems in Tunisia by endocrine disrupting compounds (EDCs) is due to anthropogenic impacts through non‐treated industrial wastewater and municipal‐treated wastewater discharge. The aim of this study was to assess the estrogenic activity of environmental matrices involving raw industrial wastewater (WW), surface water (SW), and treated wastewater (TWW). A modified E‐screen assay was used to evaluate estrogenic activity of the samples in MCF‐7 breast cancer cells. Western blotting and real‐time PCR molecular approaches were applied to investigate the mechanism of EDC‐induced MCF‐7 breast cancer cell proliferation which interferes with estrogen receptor alpha (ERα) and c‐Jun. Our results revealed a dose‐dependent estrogenic activity reaching an optimal level at a concentration of 0.01% for industrial WW and SW and 10% for TWW. Monitoring ERα protein and c‐Jun gene expression levels after 1, 6, 24, and 48 h of treatment showed a correlation in the overexpression of ERα and c‐Jun most of the time. In fact, ERα and c‐Jun correlated up‐regulation began after 1 h of treatment for industrial WW, TWW, and one SW sample. For the other SW samples, this correlated up‐regulation started after 6–24 h of treatment. Results emphasized the estrogen‐like effect of our contaminated samples on MCF‐7 cells which facilitate a cross‐talk between ERα and c‐Jun factor, thereby directly regulating the expression of estrogen‐induced genes to mediate breast cancer cell growth.
Effects of personality on the opioidergic modulation of the emotion warmth‐liking
Authors: Burgdorf Christin , Rinn Constanze , Stemmler Gerhard
Published: July 2015

Neurobiological research suggests there are discrete emotion systems, which are based on separate neural pathways with specific neurotransmitters (i.e., oxytocin, opioids). So far, autonomic regulation patterns of different positive emotions could not be unambiguously characterized. Warmth‐liking, as an emotion system, is activated during interpersonal interactions and close relationships. We postulated that warmth‐liking has a specific somatovisceral signature, which is, however, qualified by individual differences in personality and attachment style. Individual differences in personality and attachment style are postulated to contribute to μ‐opiate functioning. Forty‐eight females in a heterosexual relationship, selected on the basis of attachment reports, took part in a virtual ball‐tossing game with their partners and two confederates. Participants received either the competitive μ‐opioid antagonist naltrexone (25 mg) or a placebo in a randomized double‐blind design. Social exclusion during the game reduced feelings of warmth, increased feelings of anger, and increased blood pressure and left‐ventricular contractibility, whereas social inclusion was characterized by physiological quiescence. Further analyses revealed differential effects in self‐reported feelings as well as in cardiovascular parameters as a function of attachment style. Secure attachment predicted higher levels of warmth‐liking, physiological quiescence, and less negative feelings even during social exclusion. These findings can be interpreted as evidence for psychophysiological resilience. Furthermore, naltrexone reduced feelings of warmth and increased vasoconstriction during social inclusion, especially for securely bonded participants. These findings are remarkable hints for an opioidergic modulation of the interaction between emotion and personality. J. Comp. Neurol. 524:1712–1726, 2016. © 2015 Wiley Periodicals, Inc.
Degeneration of proprioceptive sensory nerve endings in mice harboring amyotrophic lateral sclerosis–causing mutations
Authors: Vaughan Sydney K. , Kemp Zachary , Hatzipetros Theo , Vieira Fernando , Valdez Gregorio
Published: July 2015

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that primarily targets the motor system. Although much is known about the effects of ALS on motor neurons and glial cells, little is known about its effect on proprioceptive sensory neurons. This study examines proprioceptive sensory neurons in mice harboring mutations associated with ALS, in SOD1G93A and TDP43A315T transgenic mice. In both transgenic lines, we found fewer proprioceptive sensory neurons containing fluorescently tagged cholera toxin in their soma five days after injecting this retrograde tracer into the tibialis anterior muscle. We asked whether this is due to neuronal loss or selective degeneration of peripheral nerve endings. We found no difference in the total number and size of proprioceptive sensory neuron soma between symptomatic SOD1G93A and control mice. However, analysis of proprioceptive nerve endings in muscles revealed early and significant alterations at Ia/II proprioceptive nerve endings in muscle spindles before the symptomatic phase of the disease. Although these changes occur alongside those at α‐motor axons in SOD1G93A mice, Ia/II sensory nerve endings degenerate in the absence of obvious alterations in α‐motor axons in TDP43A315T transgenic mice. We next asked whether proprioceptive nerve endings are similarly affected in the spinal cord and found that nerve endings terminating on α‐motor neurons are affected during the symptomatic phase and after peripheral nerve endings begin to degenerate. Overall, we show that Ia/II proprioceptive sensory neurons are affected by ALS‐causing mutations, with pathological changes starting at their peripheral nerve endings. J. Comp. Neurol. 523:2477–2494, 2015. © 2015 Wiley Periodicals, Inc.
The effect of cooling rate on size, quality and morphology of KTiOPO4 (KTP) crystals grown by different nucleation techniques
Authors: Elaheh Gharibshahian , Majid Jafar Tafreshi
Published: July 2015

KTP crystals have been grown by two nucleation techniques namely spontaneous nucleation in flux medium and nucleation on Pt rod using K6P4O13 flux.10 °C/h, 7 °C/h, 0.8 °C/h, 0.4 °C/h and 0.2 °C/h cooling rates were applied for spontaneous nucleation and crystals up to 15 × 7 × 4 mm3 in size were grown. 1 °C/h and 0.8 °C/h cooling rates were also used for nucleation on Pt rod and crystals up to 8 × 6 × 3 mm3 in size were grown. The effect of cooling rate on size, morphology and optical quality of grown crystals by both techniques were studied. For nucleation on Pt rod upper and lower rotation rates limits and an optimum rotation rate were distinguished for each cooling rate. Quality of the grown crystals by both techniques was characterized by optical transmission analysis.
The double phosphates MIMIIPO4 (MI – Na, K; MII – Mg, Mn, Co, Ni, Zn) – synthesis from chloride melts and characterization
Authors: Livitska Oksana V. , Strutynska Nataliia Yu. , Zatovsky Igor V. , Slobodyanik Nikolay S.
Published: July 2015

The particularities of the chemical interaction in systems MIPO3‐MIIO(or Mn2O3)‐MICl (MI – Na, K; MII – Mg, Co, Ni, Zn) have been investigated at the temperature 1073 K and molar ratios P/Mx = 1 or 2 and MICl/(MIPO3 + MIIO(or Mn2O3)) = 30. The conditions of formation of complex phosphates MІMIIPO4 and Na4Ni3(PO4)2P2O7 have been found. Influences of the nature of alkali and bivalent metals on the products composition were discussed. The advantages of chloride melts using (synthesis time reduction and temperature reducing) for preparing of complex phosphates were shown. The synthesized compounds have been characterized using the powder X‐ray diffraction, Fourier transform infrared and diffuse reflectance spectroscopies.
Physicochemical quality and oxidative stability of linseed (Linum usitatissimum) and camelina (Camelina sativa) cold‐pressed oils from retail outlets
Authors: Raczyk Marianna , Popis Edyta , Kruszewski Bartosz , Ratusz Katarzyna , Rudzińska Magdalena
Published: July 2015

Cold‐pressed linseed and camelina oils have been available on the global market for many years. They are known for their high contents of polyunsaturated fatty acids (PUFAs), mainly α‐linolenic (ALA). This study aimed to evaluate the quality and oxidative stability of linseed and camelina cold‐pressed oils purchased in retail outlets in Poland. The fatty acid composition, peroxide value, acid value, anisidine value, chlorophyll pigments, carotenoid pigments, and oxidative stability of these oils were analyzed. It was found that all analyzed oils meet the standards of requirements of the Codex Alimentarius (2009) for cold‐pressed oils. The content of chlorophyll and carotenoid pigments of linseed and camelina oils was significantly different. The percentage composition of PUFAs was high in both types of oils, but it was significantly higher in linseed than in camelina oils. The results of Rancimat and PDSC tests show low oxidative stability of all tested oils, although camelina oils were more stable than linseed oils.

Practical applications: Based on the study, it can be concluded that linseed and camelina oils should be included in the human diet to provide an optimal ratio of omega‐6 to omega‐3 fatty acids. The differences between tested oils of the same seed species were significant. This study shows that quick methods of testing an oils oxidative stability (Rancimat and PDSC) may be successfully applied in oil studies.

The study evaluates the quality of cold‐pressed linseed and camelina oils purchased in retail outlets in Poland. The research shows a correlation (r = −0.673) between content of polyunsaturated fatty acids and oxidative stability of tested oils. Oxidative stability was determined by Rancimat test and fatty acid composition by GC‐FID.
Synthesis of the C‐8–C‐24 Fragment of Maltepolide C by Using a Tandem Di­hydroxylation/SN2 Cyclization Sequence
Authors: Mohapatra Debendra K. , Reddy D. Sai , Reddy G. Sudhakar , Yadav Jhillu S.
Published: July 2015

A highly stereoselective synthesis of the C‐8–C‐24 fragment of maltepolide C has been achieved in a convergent and efficient manner by utilizing a tandem Sharpless asymmetric dihydroxylation (SAD)/SN2 cyclization reaction sequence as the key step. Other important steps include a Crimmins‐modified Evans aldol reaction, a Brown asymmetric allylation, a Horner–Wadsworth–Emmons olefination, and a Corey–Bakshi–Shibata (CBS) reduction.
A Convenient Approach to CF3‐Containing N‐Heterocycles Based on 2‐Methoxy‐2‐methyl‐5‐(trifluoromethyl)furan‐3(2H)‐one
Authors: Bazhin Denis N. , Kudyakova Yulia S. , Röschenthaler Gerd‐Volker , Burgart Yanina V. , Slepukhin Pavel A. , Isenov Maksim L. , Saloutin Victor I. , Charushin Valery N.
Published: July 2015

New synthetic routes to trifluoromethylated N‐heterocycles based on condensations of 2‐methoxy‐2‐methyl‐5‐(trifluoromethyl)furan‐3(2H)‐one with bifunctional N‐nucleophiles are described. For the first time, trifluoromethyl‐containing pyrazoles, pyrazolines and isoxazolines bearing hydrazone or oxime groups could be obtained by a one‐pot strategy based on reactions of 5‐(trifluoromethyl)furan‐3(2H)‐one with two equivalents of the corresponding hydrazines or hydroxylamine in the presence of an acid. The interaction of furan‐3(2H)‐one with ureas proceeded under mild conditions to furnish 1H‐furo[2,3‐d]imidazol‐2(3H)‐one derivatives in good yield. Further, a trifluoromethyl‐containing quinoxaline derivative was obtained by condensation of furan‐3(2H)‐one with ortho‐phenylenediamine.
Distributions of return values for ocean wave characteristics in the South China Sea using directional–seasonal extreme value analysis
Authors: Randell David , Feld Graham , Ewans Kevin , Jonathan Philip
Published: July 2015

Estimation of ocean environmental return values is critical to the safety and reliability of marine and coastal structures. For ocean waves and storm severity, return values are typically estimated by extreme value analysis of time series of measured or hindcast sea state significant wave height HS. For a single location, this analysis is complicated by the serial dependence of HS in time and its non‐stationarity with respect to multiple covariates, particularly direction and season.

Here, we report a non‐stationary extreme value analysis of storm peak significant wave height
, assumed temporally independent given covariates, incorporating directional and seasonal effects using a spline‐based methodology incorporating an ensemble of models for different extreme value thresholds. Quantile regression is used to estimate suitable thresholds. For each threshold, a Poisson process is used to estimate the rate of occurrence of threshold exceedances, and a generalised Pareto model characterises the magnitude of threshold exceedances. Covariate effects are incorporated at each stage using penalised tensor products of B‐splines to give smooth model parameter variation as a function of covariates. Optimal smoothing penalties are selected using cross‐validation, and uncertainty is quantified using bias‐corrected and accelerated bootstrap resampling.

We use the model to estimate environmental return values for a location in the Makassar Strait, in the South China Sea. Return values distributions for
are estimated by simulation under the threshold ensemble model. Return values for HS are then estimated by simulating intra‐storm trajectories of HS consistent with the characteristics of the simulated storm peak events using a matching procedure. Return values for maximum individual crest elevation C are estimated by marginalisation using a pre‐specified conditional distribution for C given HS and other sea state parameters. Model validation is performed by comparing confidence intervals for cumulative distribution functions of
and HS for the period of the data with empirical sample‐based estimates. Copyright © 2015 John Wiley & Sons, Ltd.
Performance Characterization of Solid Oxide Cells Under High Pressure
Authors: Sun X. , Bonaccorso A. D. , Graves C. , Ebbesen S. D. , Jensen S. H. , Hagen A. , Holtappels P. , Hendriksen P. V. , Mogensen M. B.
Published: July 2015

In this work, recent pressurized test results of a planar Ni‐YSZ (YSZ: Yttria stabilized Zirconia) supported solid oxide cell are presented. Measurements were performed at 800 °C in both fuel cell and electrolysis mode at different pressures. A comparison of the electrochemical performance of the cell at 1 and 3 bar shows a significant and equal performance gain at higher pressure in both fuel cell mode and electrolysis mode. Electrochemical impedance spectroscopy revealed that the serial resistance was not affected by the operation pressure; all the other processes that are dependent on partial pressures (oxygen, steam and hydrogen) were affected by increasing the pressure. In electrolysis mode at low current density, the performance improvement was counteracted by the increase in open circuit voltage, but it has to be borne in mind that the pressurized gas contains higher molar free energy.
Radiotherapy did not increase thyroid cancer risk among women with breast cancer: A nationwide population‐based cohort study
Authors: Sun Li‐Min , Lin Cheng‐Li , Liang Ji‐An , Huang Wen‐Sheng , Kao Chia‐Hung
Published: July 2015

The aim of this study was to evaluate whether an increased risk of thyroid cancer exists among women with breast cancer in Taiwan, particularly among those receiving RT. We used data from the National Health Insurance system of Taiwan for the investigation. The breast cancer cohort contained 55,318 women (including 28,187 who received RT and 27,131 who received no RT), each of whom was randomly frequency matched according to age and index year with three women without breast cancer from the general population. Cox's proportion hazards regression analysis was conducted to estimate the effects of breast cancer with or without RT treatment on subsequent thyroid cancer risk. We found that women with breast cancer exhibited a significantly higher risk of subsequent thyroid cancer (adjusted hazard ratio [aHR] = 1.98, 95% confidence interval [CI] = 1.60–2.44). The two groups (with or without RT) in the breast cancer cohort exhibited significantly increased risks. However, in the breast cancer cohort, the risk of thyroid cancer among women who received RT was not significantly higher than that of women who received no RT (aHR = 1.28, 95% CI = 0.90–1.83). Stratified analysis according to age revealed that only younger women with breast cancer (20–54 y) had a significantly higher risk of developing thyroid cancer. This study determined that Taiwanese women with breast cancer had a higher risk of developing thyroid cancer; however, RT seems to not play a crucial role in this possible relationship.
Outerplanar and Planar Oriented Cliques
Authors: Nandy Ayan , Sen Sagnik , Sopena Éric
Published: July 2015

The clique number of an undirected graph G is the maximum order of a complete subgraph of G and is a well‐known lower bound for the chromatic number of G. Every proper k‐coloring of G may be viewed as a homomorphism (an edge‐preserving vertex mapping) of G to the complete graph of order k. By considering homomorphisms of oriented graphs (digraphs without cycles of length at most 2), we get a natural notion of (oriented) colorings and oriented chromatic number of oriented graphs. An oriented clique is then an oriented graph whose number of vertices and oriented chromatic number coincide. However, the structure of oriented cliques is much less understood than in the undirected case. In this article, we study the structure of outerplanar and planar oriented cliques. We first provide a list of 11 graphs and prove that an outerplanar graph can be oriented as an oriented clique if and only if it contains one of these graphs as a spanning subgraph. Klostermeyer and MacGillivray conjectured that the order of a planar oriented clique is at most 15, which was later proved by Sen. We show that any planar oriented clique on 15 vertices must contain a particular oriented graph as a spanning subgraph, thus reproving the above conjecture. We also provide tight upper bounds for the order of planar oriented cliques of girth k for all .
Novel Synthesis of 1,3‐Thiazine and Pyrimidinethione Derivatives from (1‐Aryl ethylidene)hydrazinecarbothioamides and Tetracyanoethylene
Authors: Hassan Alaa A. , Ibrahim Yusria R. , El‐Sheref Essmat M. , Bräse Stefan
Published: July 2015

1,3‐Thiazine‐5,6,6‐tricarbonitrile and 2‐thioxo‐2,3‐dihydropyrimidine‐4,5‐dicarbonitriles derivatives were synthesized via interactions between (1‐aryl ethylidene)hydrazinecarbothioamides and tetracyanoethylene to give the derivatives of tetracyanoethane and tricyanovinylation intermediates, followed by heterocyclization. The structures of the products have been confirmed by different spectroscopic analyses. A rational for the formation of the products is presented.
Internal medicine progress note writing attitudes and practices in an electronic health record
Authors: Stewart Elizabeth , Kahn Daniel , Lee Edward , Simon Wendy , Duncan Mark , Mosher Hilary , Harris Katherine , Bell John , El‐Farra Neveen , Sharpe Bradley
Published: July 2015

BACKGROUND

The electronic health record (EHR) has been viewed with both praise and skepticism. Multiple editorials have expressed concerns that EHR implementation and “efficiency tools” such as copy forward and auto population have resulted in a decrement in note accuracy, relevance, and critical thinking.

OBJECTIVE

To evaluate the perceptions of internal medicine housestaff and attendings on inpatient progress note quality at 4 academic institutions after the implementation of an EHR.

DESIGN

Cross‐sectional survey.

MEASUREMENTS

We developed surveys that assessed housestaff and attendings opinion of current progress note quality, the impact of the EHR on quality, and the purposes of a progress note.

RESULTS

We received 99 completed surveys from interns (66%), 155 from residents (49%), and 153 from attendings (70%) across 4 institutions. The majority of housestaff responded that the quality of notes was “unchanged” or “better” following the implementation of an EHR, whereas attendings believed note quality was “unchanged” or “worse.” Attendings’ perceptions of housestaff notes were significantly lower than housestaff perceptions of their own notes across all domains. With regard to the effect of copy forward and autopopulation, the majority of housestaff viewed these to be “neutral” or “somewhat positive,” whereas attendings viewed these as “neutral” or “somewhat negative.” Housestaff and attendings had nearly perfect agreement regarding the purpose of the progress note.

CONCLUSIONS

Attendings and housestaff disagree on the current quality of progress notes and the impact of an EHR on note quality, but agree on the purpose of a progress note. Journal of Hospital Medicine 2015;10:525–529. © 2015 Society of Hospital Medicine
Effect of ACL graft material on anterior knee force during simulated in vivo ovine motion applied to the porcine knee: An in vitro examination of force during 2000 cycles
Authors: Boguszewski Daniel V. , Wagner Christopher T. , Butler David L. , Shearn Jason T.
Published: July 2015

This study determined how anterior cruciate ligament (ACL) reconstruction affected the magnitude and temporal patterns of anterior knee force and internal knee moment during 2000 cycles of simulated gait. Porcine knees were tested using a six degree‐of‐freedom robot, examining three porcine allograft materials compared with the native ACL. Reconstructions were performed using: (1) bone‐patellar tendon‐bone allograft (BPTB), (2) reconstructive porcine tissue matrix (RTM), or (3) an RTM‐polymer hybrid construct (Hybrid). Forces and moments were measured over the entire gait cycle and contrasted at heel strike, mid stance, toe off, and peak flexion. The Hybrid construct performed the best, as magnitude and temporal changes in both anterior knee force and internal knee moment were not different from the native ACL knee. Conversely, the RTM knees showed greater loss in anterior knee force during 2000 cycles than the native ACL knee at heel strike and toe off, with an average force loss of 46%. BPTB knees performed the least favorably, with significant loss in anterior knee force at all key points and an average force loss of 61%. This is clinically relevant, as increases in post‐operative knee laxity are believed to play a role in graft failure and early onset osteoarthritis. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1789–1795, 2015.
Quantitative comparison of the microscopic anatomy of the human ACL femoral and tibial entheses
Authors: Beaulieu Mélanie L. , Carey Grace E. , Schlecht Stephen H. , Wojtys Edward M. , Ashton‐Miller James A.
Published: July 2015

The femoral enthesis of the human anterior cruciate ligament (ACL) is known to be more susceptible to injury than the tibial enthesis. To determine whether anatomic differences might help explain this difference, we quantified the microscopic appearance of both entheses in 15 unembalmed knee specimens using light microscopy, toluidine blue stain and image analysis. The amount of calcified fibrocartilage and uncalcified fibrocartilage, and the ligament entheseal attachment angle were then compared between the femoral and tibial entheses via linear mixed‐effects models. The results showed marked differences in anatomy between the two entheses. The femoral enthesis exhibited a 3.9‐fold more acute ligament attachment angle than the tibial enthesis (p < 0.001), a 43% greater calcified fibrocartilage tissue area (p < 0.001), and a 226% greater uncalcified fibrocartilage depth (p < 0.001), with the latter differences being particularly pronounced in the central region. We conclude that the ACL femoral enthesis has more fibrocartilage and a more acute ligament attachment angle than the tibial enthesis, which provides insight into why it is more vulnerable to failure. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1811–1817, 2015.
Shorter, rough trunnion surfaces are associated with higher taper wear rates than longer, smooth trunnion surfaces in a contemporary large head metal‐on‐metal total hip arthroplasty system
Authors: Brock Timothy M. , Sidaginamale Raghavendra , Rushton Steven , Nargol Antoni V. F. , Bowsher John G. , Savisaar Christina , Joyce Tom J. , Deehan David J. , Lord James K. , Langton David J.
Published: July 2015

Taper wear at the head‐neck junction is a possible cause of early failure in large head metal‐on‐metal (LH‐MoM) hip replacements. We hypothesized that: (i) taper wear may be more pronounced in certain product designs; and (ii) an increased abductor moment arm may be protective. The tapers of 104 explanted LH‐MoM hip replacements revised for adverse reaction to metal debris (ARMD) from a single manufacturer were analyzed for linear and volumetric wear using a co‐ordinate measuring machine. The mated stem was a shorter 12/14, threaded trunnion (n = 72) or a longer, smooth 11/13 trunnion (n = 32). The abductor moment arm was calculated from pre‐revision radiographs. Independent predictors of linear and volumetric wear included taper angle, stem type, and the horizontal moment arm. Tapers mated with the threaded 12/14 trunnion had significantly higher rates of volumetric wear (0.402 mm3/yr vs. 0.123 mm3/yr [t = −2.145, p = 0.035]). There was a trend to larger abductor moment arms being protective (p = 0.055). Design variation appears to play an important role in taper‐trunnion junction failure. We recommend that surgeons bear these findings in mind when considering the use of a short, threaded trunnion with a cobalt‐chromium head. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1868–1874, 2015.