bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Light Wavelength on Resveratrol Content and Antioxidant Capacity in Arachis hypogaeas L.
Authors: Chung Ill-Min , Lee Changhwan , Hwang Myeong Ha , Kim Seung-Hyun , Chi Hee-Yeon , Yu Chang Yeon , Chelliah Ramachandran , Oh Deog-Hwan , Ghimire Bimal Kumar
Published: February 2021

The quality and intensity of light can have a huge influence on plant growth and bioactive compound production. Compared to conventional lighting, very little is known about the influence of light emitting diodes (LED) irradiation on the antioxidant and antimicrobial properties and resveratrol content of peanut sprouts. This study was aimed at understanding the effects of LED light on the growth and antioxidant capacity of peanut sprouts. The resveratrol concentration was determined by liquid chromatography–tandem mass spectrometry. Peanut sprouts grown under blue LED light exhibited a higher total resveratrol content grown than those under fluorescent light and other LED light sources. The highest total phenolic content was recorded in the case of blue LED. The 1,1-diphenyl-2-picrylhydrazyl and 2,2-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid radical scavenging values of blue LED‒treated and micro-electrodeless light-treated sprouts were significantly (p < 0.05) higher than that of sprouts grown under lights with other wavelengths. A Pearson correlation analysis revealed a strong association of the resveratrol, total phenolic, and flavonoid contents of peanut sprouts with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS), indicating its contribution to antioxidant activities. The anti-tyrosinase activity increased with an increase in the concentration of the tested sample. Blue LED-irradiated peanut extracts at a selected concentration range showed moderate cytotoxicity. Furthermore, the antimicrobial activity of peanut sprouts grown under blue LED was effective against Escherichia coli, Klebsiella pneumonia, and Vibrio litoralis. The present study revealed that the application of LEDs during the peanut sprouts growth improves the antioxidant activity, resveratrol concentration, and metabolite accumulation.
Effect of First Long-Term Training on Whole Blood Count and Blood Clotting Parameters in Thoroughbreds
Authors: Miglio Arianna , Falcinelli Emanuela , Mezzasoma Anna Maria , Cappelli Katia , Mecocci Samanta , Gresele Paolo , Antognoni Maria Teresa
Published: February 2021

Training has a strong effect on the physiology of hematological parameters and blood coagulation, both in humans and in horses. Several blood changes have been reported after exercise in horses but available data differ. We aimed to investigate modifications in complete blood count and some hemostatic parameters induced by the first training period in young untrained Thoroughbred racehorses to detect a possible labile blood coagulability in racehorses. Twenty-nine untrained 2-year-old Thoroughbreds were followed during their incremental 4-month sprint exercise schedule. Blood collection was performed once a month, five times (T-30, T0, T30, T60 and T90), before and during the training period for measurement of complete blood count (CBC) and blood clotting parameters (prothrombin time—PT, activated partial prothrombin time—APTT, thrombin clotting time—TCT, fibrinogen—Fb, thrombin–antithrombin complex—TAT). Differences among the time points for each parameter were analyzed (ANOVA, Kruskal–Wallis one-way analysis of variance, p < 0.05). In Thoroughbreds, the first long-term exercise workout period was found to induce a statistical increase in red blood cell indexes and lymphocytes, eosinophils and platelet counts, as well as a hypercoagulability state evident at 30 days of training, which returned to basal levels after 90 days. Regular physical exercise seems to blunt the negative effects of acute efforts on hematological and clotting parameters, an effect that may be attributed to the training condition.
Allium-Based Phytobiotic Enhances Egg Production in Laying Hens through Microbial Composition Changes in Ileum and Cecum
Authors: Rabelo-Ruiz Miguel , Ariza-Romero Juan José , Zurita-González María Jesús , Martín-Platero Antonio Manuel , Baños Alberto , Maqueda Mercedes , Valdivia Eva , Martínez-Bueno Manuel , Peralta-Sánchez Juan Manuel
Published: February 2021

Phytobiotics (bioactive compounds extracted from plants) are one of the explored alternatives to antibiotics in poultry and livestock due to their antimicrobial activity and its positive effects on gut microbiota and productive properties. In this study, we supplemented a product based on garlic and onion compounds in the diet to laying hens at the beginning of their productive life (from 16 to 20 weeks post-hatching). The experimental group showed a significant increase in the number of eggs laid and in their size, produced in one month compared to the control. This increase in production was accompanied by microbiota changes in the ileum and cecum by means of high throughput sequencing analyses. These bacterial shifts in the ileum were mainly the result of compositional changes in the rare biosphere (unweighted UniFrac), while in the cecum, treatment affected both majority and minority bacterial groups (weighted and unweighted UniFrac). These changes in the microbiota suggest an improvement in food digestibility. The relative abundance of Lactococcus in the ileum and Lactobacillus in the cecum increased significantly in the experimental group. The relative abundance of these bacterial genera are known to have positive effects on the hosts. These results are very promising for the use of these compounds in poultry for short periods.
In Vitro Assessment of Enteric Methane Emission Potential of Whole-Plant Barley, Oat, Triticale and Wheat
Authors: Aboagye Isaac A. , Rosser Christine L. , Baron Vern S. , Beauchemin Karen A.
Published: February 2021

The study determined in vitro enteric methane (CH4) emission potential of whole-plant cereal (WPC) forages in relationship to nutrient composition, degradability, and rumen fermentation. Two varieties of each WPC (barley, oat, triticale, and wheat) were harvested from two field replications in each of two locations in central Alberta, Canada, and an in vitro batch culture technique was used to characterize gas production (GP), fermentation, and degradability. Starch concentration (g/kg dry matter (DM)) was least (p < 0.001) for oat (147), greatest for wheat (274) and barley (229), and intermediate for triticale (194). The aNDF concentration was greater for oat versus the other cereals (531 vs. 421 g/kg DM, p < 0.01). The 48 h DM and aNDF degradabilities (DMD and aNDFD) differed (p < 0.001) among the WPCs. The DMD was greatest for barley, intermediate for wheat and triticale, and least for oat (719, 677, 663, and 566 g/kg DM, respectively). Cumulative CH4 production (MP; mL) from 12 h to 48 h of incubation was less (p < 0.001) for oat than the other cereals, reflecting its lower DMD. However, CH4 yield (MY; mg of CH4/g DM degraded) of barley and oat grown at one location was less than that of wheat and triticale (28 vs. 31 mg CH4/g DM degraded). Chemical composition failed to explain variation in MY (p = 0.35), but it explained 45% of the variation in MP (p = 0.02). Variation in the CH4 emission potential of WPC was attributed to differences in DMD, aNDFD, and fermentation end-products (R2 ≥ 0.88; p < 001). The results indicate that feeding whole-plant oat forage to ruminants may decrease CH4 emissions, but animal performance may also be negatively affected due to lower degradability, whereas barley forage may ameliorate emissions without negative effects on animal performance.
Elizabethkingia Intra-Abdominal Infection and Related Trimethoprim-Sulfamethoxazole Resistance: A Clinical-Genomic Study
Authors: Teng Ling-Chiao , Wang Jiunn-Min , Lu Hsueh-Yin , Mao Yan-Chiao , Lai Kuo-Lung , Tseng Chien-Hao , Huang Yao-Ting , Liu Po-Yu
Published: February 2021

(1) Background: Elizabethkingia spp. is an emerging nosocomial pathogen which causes mostly blood stream infection and nosocomial pneumonia. Among Elizabethkingia species, Elizabethkingia anophelis is the major pathogen, but misidentification as Elizabethkingia meningoseptica is a common problem. Elizabethkingia also possesses broad antibiotic resistance, resulting in high morbidity and mortality of the infection. The aim of our study was to review Elizabethkingia intra-abdominal infections and investigate resistance mechanisms against TMP/SMX in Elizabethkingia anophelis by whole genome sequencing. (2) Methods: We retrospectively searched records of patients with Elizabethkingia intra-abdominal infection between 1990 and 2019. We also conducted whole genome sequencing for a TMP/SMX-resistant Elizabethkingia anophelis to identify possible mechanisms of resistance. (3) Results: We identified a total of nine cases of Elizabethkingia intra-abdominal infection in a review of the literature, including our own case. The cases included three biliary tract infections, three CAPD-related infection, two with infected ascites, and two postoperation infections. Host factor, indwelling-catheter, and previous invasive procedure, including surgery, play important roles in Elizabethkingia infection. Removal of the catheter is crucial for successful treatment. Genomic analysis revealed accumulated mutations leading to TMP/SMX-resistance in folP. (4) Conclusions: Patients with underlying disease and indwelling catheter are more susceptible to Elizabethkingia intra-abdominal infection, and successful treatment requires removal of the catheter. The emerging resistance to TMP/SMX may be related to accumulated mutations in folP.
Oxidative Stress Boosts the Uptake of Cerium Oxide Nanoparticles by Changing Brain Endothelium Microvilli Pattern
Authors: Dal Magro Roberta , Vitali Agostina , Fagioli Stefano , Casu Alberto , Falqui Andrea , Formicola Beatrice , Taiarol Lorenzo , Cassina Valeria , Marrano Claudia Adriana , Mantegazza Francesco , Anselmi-Tamburini Umberto , Sommi Patrizia , Re Francesca
Published: February 2021

Vascular oxidative stress is considered a worsening factor in the progression of Alzheimer’s disease (AD). Increased reactive oxygen species (ROS) levels promote the accumulation of amyloid-β peptide (Aβ), one of the main hallmarks of AD. In turn, Aβ is a potent inducer of oxidative stress. In early stages of AD, the concomitant action of oxidative stress and Aβ on brain capillary endothelial cells was observed to compromise the blood–brain barrier functionality. In this context, antioxidant compounds might provide therapeutic benefits. To this aim, we investigated the antioxidant activity of cerium oxide nanoparticles (CNP) in human cerebral microvascular endothelial cells (hCMEC/D3) exposed to Aβ oligomers. Treatment with CNP (13.9 ± 0.7 nm in diameter) restored basal ROS levels in hCMEC/D3 cells, both after acute or prolonged exposure to Aβ. Moreover, we found that the extent of CNP uptake by hCMEC/D3 was +43% higher in the presence of Aβ. Scanning electron microscopy and western blot analysis suggested that changes in microvilli structures on the cell surface, under pro-oxidant stimuli (Aβ or H2O2), might be involved in the enhancement of CNP uptake. This finding opens the possibility to exploit the modulation of endothelial microvilli pattern to improve the uptake of anti-oxidant particles designed to counteract ROS-mediated cerebrovascular dysfunctions.
Ursodeoxycholic Acid May Inhibit Environmental Aging-Associated Hyperpigmentation
Authors: Moon Ik Jun , Yoo Hanju , Paik Seung Hwan , Kim Hak Tae , Kim Su Yeon , Song Youngsup , Chang Sung Eun
Published: February 2021

Extrinsic aging of the skin caused by ultraviolet (UV) light or particulate matter is often manifested by hyperpigmentation due to increased melanogenesis in senescent skin. Ursodeoxycholic acid (UDCA), which has been commonly used as a health remedy for liver diseases, is known to possess antioxidant properties. This study was done to investigate whether UDCA inhibits cellular aging processes in the cells constituting human skin and it reduces melanin synthesis. ROS, intracellular signals, IL-1α, IL-8, TNF-α, cyclooxygenase (COX)-2, type I collagen, and matrix metalloproteinases (MMPs) levels were measured in human dermal fibroblasts treated with or without UDCA after UV exposure. Melanin levels and mechanistic pathways for melanogenesis were investigated. UDCA decreased ROS, senescence-associated secretory phenotype (SASP), and proinflammatory cytokines induced by UV treatment. UDCA reduced melanogenesis in normal human melanocytes cocultured with skin constituent cells. Our results suggest that UDCA could be a comprehensive agent for the treatment of environmental aging-associated hyperpigmentation disorders.
Comparison of Anti-Oxidative Effect of Human Adipose- and Amniotic Membrane-Derived Mesenchymal Stem Cell Conditioned Medium on Mouse Preimplantation Embryo Development
Authors: Ra Kihae , Oh Hyun Ju , Kim Eun Young , Kang Sung Keun , Ra Jeong Chan , Kim Eui Hyun , Park Se Chang , Lee Byeong Chun
Published: February 2021

Oxidative stress is a major cause of damage to the quantity and quality of embryos produced in vitro. Antioxidants are usually supplemented to protect embryos from the suboptimal in vitro culture (IVC) environment. Amniotic membrane-derived mesenchymal stem cells (AMSC) have emerged as a promising regenerative therapy, and their paracrine factors with anti-oxidative effects are present in AMSC conditioned medium (CM). We examined the anti-oxidative potential of human AMSC-CM treatment during IVC on mouse preimplantation embryo development and antioxidant gene expression in the forkhead box O (FoxO) pathway. AMSC-CM (10%) was optimal for overall preimplantation embryo developmental processes and upregulated the expression of FoxOs and their downstream antioxidants in blastocysts (BL). Subsequently, compared to adipose-derived mesenchymal stem cell (ASC)-CM, AMSC-CM enhanced antioxidant gene expression and intracellular GSH levels in the BL. Total antioxidant capacity and SOD activity were greater in AMSC-CM than in ASC-CM. Furthermore, SOD and catalase were more active in culture medium supplemented with AMSC-CM than in ASC-CM. Lastly, the anti-apoptotic effect of AMSC-CM was observed with the regulation of apoptosis-related genes and mitochondrial membrane potential in BL. In conclusion, the present study established AMSC-CM treatment at an optimal concentration as a novel antioxidant intervention for assisted reproduction.
Knee MRI Underestimates the Grade of Cartilage Lesions
Authors: Krakowski Przemysław , Karpiński Robert , Jojczuk Mariusz , Nogalska Agata , Jonak Józef
Published: February 2021

Purpose: This study was conducted in order to evaluate the clinical utility of MRI in detecting cartilage lesions and its dependence on anatomical location and lesion grade. Methods: A retrospective analysis of MRI reports and arthroscopic findings was performed on 190 consecutive patients treated in one orthopaedic department. MRI protocols were prepared by 18 radiologists from 10 different MRI centers with the use of 1.5 T magnets. The image protocols were selected by reading radiologists. Four hundred and fifty-three chondral lesions in five anatomic locations were identified during this study and graded according to the ICRS classification. Sensitivity, specificity, receiver operating characteristic (ROC), and Bangdiwala’s observer agreement charts were utilized to evaluate the diagnostic performance. Results: Only approximately 30% of MRI showed an adequate cartilage status in all anatomical locations. The sensitivity ranged from 92% in healthy cartilage to 5% in grade I lesions. The specificity differed also grossly depending on the lesion grade, reaching 96.5% in grade four lesions and 38% in healthy cartilage. The medial compartment Bangdiwala’s observer agreement charts show a gross underestimation of cartilage lesions, and the area under the curve (AUC) of ROC surpasses 0.7 only in the medial femoral condyle and patella-femoral joint. Overall, the medial compartment accuracy was significantly higher than the lateral compartment. The MRI showed correspondence of its diagnostic performance with cartilage lesion severity. Conclusion: MRI underestimates the extent of cartilage injury and evaluation of cartilage defects based on MRI should be taken with caution by orthopaedic surgeons in planning surgery. Surgical planning on MRI should take cartilage lesions under consideration, even if no cartilage lesions are reported on the MRI.
An Integrated Thermal-Electrical Model for Simulations of Battery Behavior in CubeSats
Authors: Vega Martinez Sara , Filho Edemar Morsch , Seman Laio Oriel , Bezerra Eduardo Augusto , Nicolau Vicente de Paulo , Ovejero Raúl García , Leithardt Valderi Reis Quietinho
Published: February 2021

This work presents an integrated thermal-electrical simulation model, capable of taking into account the thermal and electrical effects of the battery and photovoltaic panels for each instant of time in a given orbit and attitude. Using the physical equations that govern the thermal and electrical models involved during a CubeSat operation, the proposed integrated model can estimate the temperature and energy conditions of the battery, not only in an isolated way but also in considering the mutual effects on the system. Besides, special attention is given to photovoltaic panels used in the energy harvesting process, whose performance is affected by irradiance and temperature along the orbit. The integrated model can be useful for engineers when developing the subsystems of their CubeSats, taking into account, for example, the battery temperature control through a heater. Simulations were performed to illustrate the functioning of the proposed model with variations in the power requirements of its modules and the temperature of the battery throughout the orbit, and a heater’s influence on it.
A Study on the Dynamic Behavior of a Vertical Tunnel Shaft Embedded in Liquefiable Ground during Earthquakes
Authors: Kwon Sun Yong , Yoo Mintaek
Published: February 2021

Since liquefaction was first observed in South Korea during the Pohang earthquake, public concerns regarding the seismic stability of major infrastructure have increased substantially. However, the seismic behavior of tunnel shafts, which are an important element of tunnel structures, has not been properly established, especially under liquefiable soil conditions. In this study, 3D numerical modeling with Fast Lagrangian Analysis of Continua in 3 Dimensions (FLAC3D) was performed to predict the dynamic behavior of a vertical tunnel shaft during liquefaction. This study demonstrates key aspects of the dynamic behavior of tunnel shafts by varying important parameters such as the thickness of the liquefiable soil layer and applied seismicity level. Moreover, important dynamic responses such as excess pore pressure generation, the seismic bending moment of the shaft, and lateral displacements are highlighted. Finally, meaningful discussion of the seismic risk analysis based on damage indices is conducted based on the analysis results.
A Method for Rainfall Detection and Rainfall Intensity Level Retrieval from X-Band Marine Radar Images
Authors: Lu Zhizhong , Sun Lei , Zhou Ying
Published: February 2021

Currently, it is a hot research topic to retrieve the wave parameters by using X-band marine radar. However, the rainfall noise usually exists in the collected marine radar images, which seriously interferes with the extraction of the wave parameters. To reduce the influence of rainfall noise, the zero-pixel percentage (ZPP) method is widely used to detect rainfall in radar images, but the detection accuracy is limited, and the selection of the threshold needs to be further studied. Based on the ZPP method, the ratio of zero intensity to echo (RZE) method for rainfall detection is proposed in this paper. The detection threshold is determined by statistical analysis of a large amount of radar data. Additionally, it is proposed for the first time to retrieve the rainfall intensity level from X-band marine radar images. In addition, the concept of the occlusion area is proposed. The proposed area and the wave area are used as the rainfall detection area of the radar image, respectively, for experimental research. The data obtained from the Pingtan experimental base in Fujian Province are used to verify the effectiveness of the proposed method. The experimental results show that the detection accuracy of the proposed method is 11.7% higher than that of the ZPP method, and the accuracy of rainfall intensity level retrieval is 84%.
Shear Behavior of Concrete Encased Steel Truss Composite Girders
Authors: Lim Chisung , Choi Seung-Ho , Oh Jae Yuel , Han Sun-Jin , Lee Moon-Sung , Kim Kang Su
Published: February 2021

In this study, experimental tests were performed to evaluate the shear performance of encased steel truss (EST) composite girders that can resist loads at construction and composite stages. Four full-scale EST composite girders were fabricated, where the truss type (Pratt truss and Warren truss) and presence of stirrups were set as main test variables. The test results showed that in specimens applied with the Pratt truss, horizontal shear cracking occurring along the interface between concrete and steel was the dominant failure mode. Based on the crack pattern and failure plane observed from the test, the horizontal shear strengths of the Pratt truss specimens were calculated, which provided conservative results. On the other hand, in the specimens with the Warren truss inside, the strengths of the specimens were governed by the shear failure occurring in the screw rod connecting the truss elements prior to the yielding of the diagonal member. The shear strengths of the Warren truss specimens calculated based on the shear failure of the screw rod were similar to that obtained from the test.
Application of Lactiplantibacillus plantarum SCH1 for the Bioconservation of Cooked Sausage Made from Mechanically Separated Poultry Meat
Authors: Łaszkiewicz Beata , Szymański Piotr , Zielińska Dorota , Kołożyn-Krajewska Danuta
Published: February 2021

The aim of the research was an assessment of the effect of the Lactiplantibacillus plantarum SCH1 strain isolated from ecological raw fermented pork roast on pH, redox potential, nitrites, and nitrates content, L a* b* color parameters, total heme pigments content, nitrosyl myoglobin concentration, as well as the microbiological quality and sensory traits of cooked sausages produced from mechanically separated poultry meat (MSPM), cured with a lower sodium nitrite level (NaNO2 50 mg/kg) after production as well as after storage (1 and 3 weeks of storage). The biochemical identification of the Lactobacillus bacteria after storage was also performed. Tests were performed in two sausage treatments: C—control sausage made from MSPM and L—sausage made from MSPM inoculated with L. plantarum at approx. 107 cfu/g. No negative effect of using the L. plantarum SCH1 strain on the physical and chemical MSPM sausage features was found. The treatment with L. plantarum SCH1 was of better microbiological quality after 3 weeks of storage. The sausages with L. plantarum SCH1 kept good sensory quality while the control treatment was spoiled after storage.
Cascading Operators in CAT(0) Spaces
Authors: Fetter Nathansky Helga , Villada Bedoya Jeimer
Published: February 2021

In this work, we introduce the notion of cascading non-expansive mappings in the setting of CAT(0) spaces. This family of mappings properly contains the non-expansive maps, but it differs from other generalizations of this class of maps. Considering the concept of Δ-convergence in metric spaces, we prove a principle of demiclosedness for this type of mappings and a Δ-convergence theorem for a Mann iteration process defined using cascading operators.
Decision Trees for Predicting Mortality in Transcatheter Aortic Valve Implantation
Authors: Mamprin Marco , Zelis Jo M. , Tonino Pim A. L. , Zinger Sveta , de With Peter H. N.
Published: February 2021

Current prognostic risk scores in cardiac surgery do not benefit yet from machine learning (ML). This research aims to create a machine learning model to predict one-year mortality of a patient after transcatheter aortic valve implantation (TAVI). We adopt a modern gradient boosting on decision trees classifier (GBDTs), specifically designed for categorical features. In combination with a recent technique for model interpretations, we developed a feature analysis and selection stage, enabling the identification of the most important features for the prediction. We base our prediction model on the most relevant features, after interpreting and discussing the feature analysis results with clinical experts. We validated our model on 270 consecutive TAVI cases, reaching a C-statistic of 0.83 with CI [0.82, 0.84]. The model has achieved a positive predictive value ranging from 57% to 64%, suggesting that the patient selection made by the heart team of professionals can be further improved by taking into consideration the clinical data we identified as important and by exploiting ML approaches in the development of clinical risk scores. Our approach has shown promising predictive potential also with respect to widespread prognostic risk scores, such as logistic European system for cardiac operative risk evaluation (EuroSCORE II) and the society of thoracic surgeons (STS) risk score, which are broadly adopted by cardiologists worldwide.
Initial Identification of UDP-Glucose Dehydrogenase as a Prognostic Marker in Breast Cancer Patients, Which Facilitates Epirubicin Resistance and Regulates Hyaluronan Synthesis in MDA-MB-231 Cells
Authors: Vitale Daiana L. , Caon Ilaria , Parnigoni Arianna , Sevic Ina , Spinelli Fiorella M. , Icardi Antonella , Passi Alberto , Vigetti Davide , Alaniz Laura
Published: February 2021

UDP-glucose-dehydrogenase (UGDH) synthesizes UDP-glucuronic acid. It is involved in epirubicin detoxification and hyaluronan synthesis. This work aimed to evaluate the effect of UGDH knockdown on epirubicin response and hyaluronan metabolism in MDA-MB-231 breast cancer cells. Additionally, the aim was to determine UGDH as a possible prognosis marker in breast cancer. We studied UGDH expression in tumors and adjacent tissue from breast cancer patients. The prognostic value of UGDH was studied using a public Kaplan–Meier plotter. MDA-MB-231 cells were knocked-down for UGDH and treated with epirubicin. Epirubicin-accumulation and apoptosis were analyzed by flow cytometry. Hyaluronan-coated matrix and metabolism were determined. Autophagic-LC3-II was studied by Western blot and confocal microscopy. Epirubicin accumulation increased and apoptosis decreased during UGDH knockdown. Hyaluronan-coated matrix increased and a positive modulation of autophagy was detected. Higher levels of UGDH were correlated with worse prognosis in triple-negative breast cancer patients that received chemotherapy. High expression of UGDH was found in tumoral tissue from HER2--patients. However, UGDH knockdown contributes to epirubicin resistance, which might be associated with increases in the expression, deposition and catabolism of hyaluronan. The results obtained allowed us to propose UGDH as a new prognostic marker in breast cancer, positively associated with development of epirubicin resistance and modulation of extracellular matrix.
Lower Functional and Proportional Characteristics of Cord Blood Treg of Male Newborns Compared with Female Newborns
Authors: Černý Viktor , Novotná Olga , Petrásková Petra , Hudcová Kateřina , Boráková Kristýna , Prokešová Ludmila , Kolářová Libuše , Hrdý Jiří
Published: February 2021

Understanding the early events involved in the induction of immune tolerance to harmless environmental antigens and microbiota compounds could reveal potential targets for allergic disease therapy or prevention. Regulatory T cells (Treg), particularly induced Treg (iTreg), are crucial for the induction and maintenance of tolerance against environmental antigens including allergens. A decrease in the number and/or function of Treg or iTreg could represent an early predictor of allergy development. We analyzed proportional and functional properties of Treg in the cord blood of children of allergic mothers (neonates at high risk of allergy development) and healthy mothers (neonates with relatively low risk of allergy development). We observed a higher number of induced Treg in the cord blood of females compared to males, suggesting an impaired capacity of male immunity to set up tolerance to allergens, which could contribute to the higher incidence of allergy observed in male infants. The decreased proportion of iTreg in cord blood compared with maternal peripheral blood documents the general immaturity of the neonatal immune system. We observed a positive correlation in the demethylation of the Treg-specific demethylated region (TSDR) and the proportion of Treg in cord blood. Our data suggest that immaturity of the neonatal immune system is more severe in males, predisposing them to increased risk of allergy development.
Vitamin D and Its Role in the Lipid Metabolism and the Development of Atherosclerosis
Authors: Surdu Andrei Mihai , Pînzariu Oana , Ciobanu Dana-Mihaela , Negru Alina-Gabriela , Căinap Simona-Sorana , Lazea Cecilia , Iacob Daniela , Săraci George , Tirinescu Dacian , Borda Ileana Monica , Cismaru Gabriel
Published: February 2021

Vitamin D, a crucial hormone in the homeostasis and metabolism of calcium bone, has lately been found to produce effects on other physiological and pathological processes genomically and non-genomically, including the cardiovascular system. While lower baseline vitamin D levels have been correlated with atherogenic blood lipid profiles, 25(OH)D supplementation influences the levels of serum lipids in that it lowers the levels of total cholesterol, triglycerides, and LDL-cholesterol and increases the levels of HDL-cholesterol, all of which are known risk factors for cardiovascular disease. Vitamin D is also involved in the development of atherosclerosis at the site of the blood vessels. Deficiency of this vitamin has been found to increase adhesion molecules or endothelial activation and, at the same time, supplementation is linked to the lowering presence of adhesion surrogates. Vitamin D can also influence the vascular tone by increasing endothelial nitric oxide production, as seen in supplementation studies. Deficiency can lead, at the same time, to oxidative stress and an increase in inflammation as well as the expression of particular immune cells that play a pivotal role in the development of atherosclerosis in the intima of the blood vessels, i.e., monocytes and macrophages. Vitamin D is also involved in atherogenesis through inhibition of vascular smooth muscle cell proliferation. Furthermore, vitamin D deficiency is consistently associated with cardiovascular events, such as myocardial infarction, STEMI, NSTEMI, unstable angina, ischemic stroke, cardiovascular death, and increased mortality after acute stroke. Conversely, vitamin D supplementation does not seem to produce beneficial effects in cohorts with intermediate baseline vitamin D levels.
Sustainable and Environmental Catalysis
Authors: Tabanelli Tommaso , Cespi Daniele , Cucciniello Raffaele
Published: February 2021

Over the last few decades, an increasing amount of interest from academia and industry has been devoted to the application of the Twelve Principles of the Green Chemistry in order to pursue the Sustainable Development Goals (SDGs) recommended by the United Nations [...]
Visible Light Driven Photocatalytic Decolorization and Disinfection of Water Employing Reduced TiO2 Nanopowders
Authors: Kang Xiaolan , Berberidou Chrysanthi , Galeckas Augustinas , Bazioti Calliope , Sagstuen Einar , Norby Truls , Poulios Ioannis , Chatzitakis Athanasios
Published: February 2021

Defect-engineering of TiO2 can have a major impact on its photocatalytic properties for the degradation of persisting and non-biodegradable pollutants. Herein, a series of intrinsic and extrinsic defects are induced by post annealing of crystalline TiO2 under different reducing atmospheres. A detailed optoelectronic characterization sheds light on the key characteristics of the defect-engineered TiO2 nanopowders that are linked to the photocatalytic performance of the prepared photocatalysts. The photodegradation of a model dye, malachite green, as well as the inactivation of bacterial endospores of the Geobacillus stearothermophilus species were studied in the presence of the developed catalysts under visible light illumination. Our results indicate that a combination of certain defects is necessary for the improvement of the photocatalytic process for water purification and disinfection under visible light.
Influence of Carbon Content in Ni-Doped Mo2C Catalysts on CO Hydrogenation to Mixed Alcohol
Authors: Hao Zhenjiong , Li Xiaoshen , Tian Ye , Ding Tong , Yang Guohui , Ma Qingxiang , Tsubaki Noritatsu , Li Xingang
Published: February 2021

Herein, we synthesize the Ni-doped Mo2C catalysts by a one-pot preparation method to illuminate the effect of the number of carbon atoms in Mo2C lattice on CO hydrogenation to mixed alcohol. The Ni doping inhibits the agglomeration of Mo2C crystals into large particles and the surface carbon deposition, which increase the active surface area. In addition, the interaction between Ni and Mo increases the electron cloud density of Mo species and promotes the non-dissociative adsorption and insertion of CO. Especially, our results indicate that with the increase of the nickel content, the number of carbon atoms in Mo2C lattice on the surface of the catalyst shows a volcano type variation. The low carbon content induces the formation of coordination unsaturated molybdenum species which exhibit the higher catalytic activity and mixed alcohol selectivity than other molybdenum species. Among the catalysts, the MC-Ni-1.5 catalyst with Ni/Mo molar ratio of 1.5:8.5, which has the largest amount of coordination unsaturated molybdenum species, shows the highest space-time yield of mixed alcohols, which is three times higher than that of the Mo2C catalyst.
Can Be miR-126-3p a Biomarker of Premature Aging? An Ex Vivo and In Vitro Study in Fabry Disease
Authors: Lo Curto Alessia , Taverna Simona , Costa Maria Assunta , Passantino Rosa , Augello Giuseppa , Adamo Giorgia , Aiello Anna , Colomba Paolo , Zizzo Carmela , Zora Marco , Accardi Giulia , Candore Giuseppina , Francofonte Daniele , Di Chiara Tiziana , Alessandro Riccardo , Caruso Calogero , Duro Giovanni , Cammarata Giuseppe
Published: February 2021

Fabry disease (FD) is a lysosomal storage disorder (LSD) characterized by lysosomal accumulation of glycosphingolipids in a wide variety of cytotypes, including endothelial cells (ECs). FD patients experience a significantly reduced life expectancy compared to the general population; therefore, the association with a premature aging process would be plausible. To assess this hypothesis, miR-126-3p, a senescence-associated microRNA (SA-miRNAs), was considered as an aging biomarker. The levels of miR-126-3p contained in small extracellular vesicles (sEVs), with about 130 nm of diameter, were measured in FD patients and healthy subjects divided into age classes, in vitro, in human umbilical vein endothelial cells (HUVECs) “young” and undergoing replicative senescence, through a quantitative polymerase chain reaction (qPCR) approach. We confirmed that, in vivo, circulating miR-126 levels physiologically increase with age. In vitro, miR-126 augments in HUVECs underwent replicative senescence. We observed that FD patients are characterized by higher miR-126-3p levels in sEVs, compared to age-matched healthy subjects. We also explored, in vitro, the effect on ECs of glycosphingolipids that are typically accumulated in FD patients. We observed that FD storage substances induced in HUVECs premature senescence and increased of miR-126-3p levels. This study reinforces the hypothesis that FD may aggravate the normal aging process.
Retrograde and Anterograde Transport of Lat-Vesicles during the Immunological Synapse Formation: Defining the Finely-Tuned Mechanism
Authors: Saez Juan , Dogniaux Stephanie , Shafaq-Zadah Massiullah , Johannes Ludger , Hivroz Claire , Zucchetti Andrés
Published: February 2021

LAT is an important player of the signaling cascade induced by TCR activation. This adapter molecule is present at the plasma membrane of T lymphocytes and more abundantly in intracellular compartments. Upon T cell activation the intracellular pool of LAT is recruited to the immune synapse (IS). We previously described two pathways controlling LAT trafficking: retrograde transport from endosomes to the TGN, and anterograde traffic from the Golgi to the IS. We address the specific role of four proteins, the GTPase Rab6, the t-SNARE syntaxin-16, the v-SNARE VAMP7 and the golgin GMAP210, in each pathway. Using different methods (endocytosis and Golgi trap assays, confocal and TIRF microscopy, TCR-signalosome pull down) we show that syntaxin-16 is regulating the retrograde transport of LAT whereas VAMP7 is regulating the anterograde transport. Moreover, GMAP210 and Rab6, known to contribute to both pathways, are in our cellular context, specifically and respectively, involved in anterograde and retrograde transport of LAT. Altogether, our data describe how retrograde and anterograde pathways coordinate LAT enrichment at the IS and point to the Golgi as a central hub for the polarized recruitment of LAT to the IS. The role that this finely-tuned transport of signaling molecules plays in T-cell activation is discussed.
The Effectiveness of Lurasidone Add-On for Residual Aggressive Behavior and Obsessive Symptoms in Antipsychotic-Treated Children and Adolescents with Tourette Syndrome: Preliminary Evidence from a Case Series
Authors: Colizzi Marco , Bortoletto Riccardo , Zoccante Leonardo
Published: February 2021

Children and adolescents with Tourette syndrome may suffer from comorbid psychological and behavioral difficulties, primarily Attention-Deficit Hyperactivity Disorder-related manifestations including impulsive, aggressive, and disruptive behavior, and Obsessive-Compulsive Disorder-related disturbances. Often, such additional problems represent the major cause of disability, requiring their prioritization above the tic symptomatology. Here, we present six cases of children and adolescents with treatment-resistant Tourette syndrome aged 11–17 years, whose symptoms, especially the non-tic symptoms such as aggressive behavior and obsessive symptoms, failed to respond adequately to at least two different antipsychotics and, where deemed appropriate, to a combination with a medication with a different therapeutic indication or chemical class (e.g., antidepressant or anticonvulsant). Such symptomatic manifestations were significantly reduced by the time of the subsequent control visit planned 30 days later, by using lurasidone as an add-on therapy to risperidone or aripiprazole (all p ≤ 0.009). No significant neuromotor or metabolic side effects were reported in all cases in a follow-up period ranging from 4 months to 6 months, supporting the stability of the observed clinical improvement. While still investigational, the preliminary evidence presented here gives reason to hope that lurasidone could possibly be an effective option in Tourette syndrome, warranting further investigation of its potential benefits in neurodevelopmental conditions.
Combined Heat Transfer Mechanisms in the Porous Char Layer Formed from the Intumescent Coatings under Fire
Authors: Zhang Lingyun , Hu Yupeng , Li Minghai
Published: February 2021

This study examines the combined heat transfer by thermal conduction, natural convection and surface radiation in the porous char layer that is formed from the intumescent coating under fire. The results show that some factors, such as the Rayleigh number, conductivity ratio, emissivity, radiation–conduction number, void fraction and heating mode have a certain effect on the total heat transfer. In addition, the natural convection of the air in the cavity always inhibits surface radiation among the solid walls and thermal conduction, and the character of the total heat transfer is the competition result of the three heat transfer mechanisms.
Unmodified Titanium Dioxide Nanoparticles as a Potential Contrast Agent in Photon Emission Computed Tomography
Authors: Khalid Awais , Ahmad Pervaiz , Alharthi Abdulrahman I. , Muhammad Saleh , Khandaker Mayeen Uddin , Iqbal Faruque Mohammad Rashed , Din Israf Ud , Alotaibi Mshari A.
Published: February 2021

Highly crystalline titanium dioxide nanoparticles (TiO2-NPs) are synthesized via a simple hydrothermal technique. After structural and compositional analysis, the as-synthesized unmodified TiO2-NPs are tested for improvement in two modes of kilovoltage radiation therapy and single-photon emission computed tomography (SPECT)/computed tomography (CT). Our results show that the unmodified TiO2-NPs provide an observable enhancement in CT scan image contrast ranging from 0 ± 3 HU (without NPs) to 283.7 ± 3 HU (0.23 g/mL). TiO2-NPs has excellent biocompatibility, selective uptake at target sites, and reduced toxicity. The unmodified TiO2-NPs as a contrast agent can significantly improve the existing methods of diagnosing and treating cancer.
Crystal Structures of New Ivermectin Pseudopolymorphs
Authors: Shubin Kirill , Bērziņš Agris , Belyakov Sergey
Published: February 2021

New pseudopolymorphs of ivermectin (IVM), a potential anti-COVID-19 drug, were prepared. The crystal structure for three pseudopolymorphic crystalline forms of IVM has been determined using single-crystal X-ray crystallographic analysis. The molecular conformation of IVM in crystals has been compared with the conformation of isolated molecules modeled by DFT calculations. In a solvent with relatively small molecules (ethanol), IVM forms monoclinic crystal structure (space group I2), which contains two types of voids. When crystallized from solvents with larger molecules, like γ-valerolactone (GVL) and methyl tert-butyl ether (MTBE), IVM forms orthorhombic crystal structure (space group P212121). Calculations of the lattice energy indicate that interactions between IVM and solvents play a minor role; the main contribution to energy is made by the interactions between the molecules of IVM itself, which form a framework in the crystal structure. Interactions between IVM and molecules of solvents were evaluated using Hirshfeld surface analysis. Thermal analysis of the new pseudopolymorphs of IVM was performed by differential scanning calorimetry and thermogravimetric analysis.
High-Resolution Nerve Ultrasound Abnormalities in POEMS Syndrome—A Comparative Study
Authors: Dörner Marc , Ceanga Mihai , Schreiber Frank , Stahl Jan-Hendrik , Kronlage Cornelius , Wittlinger Julia , Kramer Magdalena , Willikens Sophia , Schreiber Stefanie , Grimm Alexander , Winter Natalie
Published: February 2021

Background: High-resolution nerve ultrasound (HRUS) has been proven to be a valuable tool in the diagnosis of immune-mediated neuropathies, such as chronic inflammatory demyelinating polyradiculoneuropathy (CIDP). POEMS syndrome (polyneuropathy, organomegaly, endocrinopathy, M-protein, skin changes) is an important differential diagnosis of CIDP. Until now, there have been no studies that could identify specific HRUS abnormalities in POEMS syndrome patients. Thus, the aim of this study was to assess possible changes and compare findings with CIDP patients. Methods: We retrospectively analyzed HRUS findings in three POEMS syndrome and ten CIDP patients by evaluating cross-sectional nerve area (CSA), echogenicity and additionally calculating ultrasound pattern scores (UPSA, UPSB, UPSC and UPSS) and homogeneity scores (HS). Results: CIDP patients showed greater CSA enlargement and higher UPSS (median 14 vs. 11), UPSA (median 11.5 vs. 8) and HS (median 5 vs. 3) compared with POEMS syndrome patients. However, every POEMS syndrome patient illustrated enlarged nerves exceeding reference values, which were not restricted to entrapment sites. In CIDP and POEMS syndrome, heterogeneous enlargement patterns could be identified, such as inhomogeneous, homogeneous and regional nerve enlargement. HRUS in CIDP patients visualized both increased and decreased echointensity, while POEMS syndrome patients pictured hypoechoic nerves with hyperechoic intraneural connective tissue. Discussion: This is the first study to demonstrate HRUS abnormalities in POEMS syndrome outside of common entrapment sites. Although nerve enlargement was more prominent in CIDP, POEMS syndrome patients revealed distinct echogenicity patterns, which might aid in its differentiation from CIDP. Future studies should consider HRUS and its possible role in determining diagnosis, prognosis and treatment response in POEMS syndrome.
Current Role of Computed Tomography in the Evaluation of Acute Coronary Syndrome
Authors: Son Min Ji , Yoo Seung Min , Lee Dongjun , Lee Hwa Yeon , Song In Sup , Chun Eun Ju , White Charles S
Published: February 2021

This review article provides an overview regarding the role of computed tomography (CT) in the evaluation of acute chest pain (ACP) in the emergency department (ED), focusing on characteristic CT findings.
Laser Doppler Spectrum Analysis Based on Calculation of Cumulative Sums Detects Changes in Skin Capillary Blood Flow in Type 2 Diabetes Mellitus
Authors: Kozlov Igor , Zherebtsov Evgeny , Masalygina Galina , Podmasteryev Konstantin , Dunaev Andrey
Published: February 2021

In this article, we introduce a new method of signal processing and data analysis for the digital laser Doppler flowmetry. Our approach is based on the calculation of cumulative sums over the registered Doppler power spectra. The introduced new parameter represents an integral estimation for the redistribution of moving red blood cells over the range of speed. The prototype of the device implementing the technique is developed and tested in preliminary clinical trials. The methodology was verified with the involvement of two age groups of healthy volunteers and in a group of patients with type 2 diabetes mellitus. The main practical result of the study is the development of a set of binary linear classifiers that allow the method to identify typical patterns of the microcirculation for the healthy volunteers and diabetic patients based on the presented diagnostic algorithm.
Discovering Unwritten Stories—A Modular Case Study in Promoting Landscape Education
Authors: Lim Shaun Tyan Gin , Perono Cacciafoco Francesco
Published: February 2021

Landscapes have been and are an important aspect of any society, culture, economy and environment. Besides the role of landscape and Landscape Sciences in these arenas, there have been increasingly greater calls to incorporate landscape into the curriculum. Moreover, Landscape Education is beneficial in developing important foundations in students, particularly that of active citizenry. While the benefits of Landscape Education are evident, current research remains focused on Western, especially European, contexts. This article discusses how a leading Singapore public University incorporates Landscape Education within a relatively new module on Toponymy offered in the Linguistics and Multilingual Studies Programme. While the links between Linguistics and a course in Toponymy or even the links between Toponymy and Landscape may not be immediately apparent, an analysis of the content covered in the module demonstrates congruence to existing frameworks and principles in teaching Landscape Education and, at the same time, provides a case in point in interdisciplinarity, drawing from diverse disciplines such as Language, Linguistics, History, Geography, Landscape Sciences, Anthropological Linguistics, among many others. This study provides useful references for educational institutions in incorporating Landscape Education into their curriculum.