bluuebunny/crossref_metadata_2025 huggingface dataset:
Penehyclidine Hydrochloride Protects Rat Cardiomyocytes from Ischemia-
Reperfusion Injury by Platelet-derived Growth Factor-B
Authors: Lu Yan , Zi Congna , Zhang Liang , Cui Boqun , Li Ling , Ma Jun
Published: July 2022

Aims and Objective:

The lack of effective treatments for myocardial ischemiareperfusion
(MI-R) injury severely restricts the effectiveness of the treatment of ischemic heart
disease. In the present research, we aimed to investigate the protective effect and molecular mechanism
of penehyclidine hydrochloride (PHC) on MI-R cells.

Method:

Cell viability was quantified using CCK8. Cell apoptosis was analyzed using flow cytometry.
Western blot and Elisa assays were used for the detection of target proteins.

Result:

PHC pretreatment attenuated the inhibition of cell viability and decreased the percentage
of apoptosis induced by simulated ischemia reperfusion (SIR). Platelet-derived growth factor B
(PDGF-B) and its downstream AKT pathway were activated in PHC pretreated cells. After siRNAPDGF-
B transfection, cell viability was inhibited and apoptosis was activated in PHC pretreated
SIR cells, suggesting that PHC protected cells from SIR. PDGF-B knockdown also increased the
levels of CK, LDH, IL-6 and TNF-α in PHC pretreated SIR cells. The effect of AKT inhibitor on
H9C2 cells was consistent with that of PDGF-B knockdown.

Conclusion:

PHC pretreatment can protect cardiomyocytes from the decrease of cell activity and
the increase of apoptosis caused by reperfusion through up-regulating PDGF-B to activate PI3K
pathway. Our study indicates that PHC is a potential drug to protect cells from reperfusion injury
and PDGF-B is a potential target for preventing MI-R injury.
Extracellular Vesicles Derived from Adipose-Derived Stem Cells Facilitate
Frostbite Wound Healing By Regulating SOCS3 Expression
Authors: Zhang Nan , Yu Xin , Li Wei , Zhang Kai , Yu Jiaao , Liu Tongjun
Published: July 2022

Background:

Though adipose-derived stem cells (ADSCs) have potential applications for the
repair and regeneration of damaged tissues, limited studies have defined the function of ADSCs on dermal
fibroblasts. Our RNA-seq sequencing identified differentially expressed SOCS3 in frostbite injury.

Objective:

In the current study, we aim to examine the hypothesis that extracellular vesicles derived from
adipose-derived mesenchymal stem cells (ADSCs-EVs) may modulate SOCS3/TGF-β1 signaling in
wound healing of frostbite injury.

Methods:

sh-SOCS3 and sh-TGF-β1 were introduced to explore the biological role of SOCS3 in frostbite
injury by detecting the proliferation and migration of human skin fibroblast (HSF) cells and the wound
healing in mice. Furthermore, the extracted ADSCs-EVs were interfered with HSF cells in vitro or injected
into the frostbitten mouse model in vivo.

Results:

Upregulation of SOCS3 occurred in the skin tissues of frostbitten mice. Compared to sh-NC, the
wound healing rate of sh-SOCS3 presented higher on day 7(31.34±4.35 vs 41.83±3.74, p < 0.05) and day
14 (63.42±6.01 vs 88.99±5.12, p < 0.05) after injury. Silencing SOCS3 can promote frostbite wound healing.
Moreover, SOCS3 downregulated TGF-β1 to suppress the proliferation and migration of HSF cells, thus
impeding the skin wound healing. Additionally, ADSCs-EVs could enhance the proliferation and migration
of HSF cells according to the results of CCK-8 assay (p < 0.05), scratch test (17.82±4.25 vs
49.78±2.54, p < 0.05) and Transwell assay (42.33±6.81 vs 91.33±7.02, p < 0.05), and regulate the expression
of SOCS3/TGF-β1. The role of ADSCs-EVs in frostbite wound healing was also confirmed in vivo.
ADSCs-EVs could promote frostbite wound healing by downregulating the expression of SOCS3 and
upregulating the expression of TGF-β1 and collagen I.

Conclusions:

Collectively, ADSCs-EVs inhibit SOCS3 and facilitate the expression of TGF-β1, which
promotes the proliferation and migration of HSF cells and subsequently enhances wound healing of frostbite
injury.
Profitability moderate the effect of operating capacity and intellectual capital on financial distress
Authors: Winata I Dewa Gde Agung , Budiasih I. G. A. N.
Published: July 2022

Financial distress is the stage of declining financial conditions experienced by the company before the occurrence of bankruptcy or liquidity. This study aims to examine the effect of operating capacity and intellectual capital on financial distress with profitability as a moderating variable. The population in this study are property and real estate companies listed on the Indonesia Stock Exchange. The sample in this study amounted to 12 property and real estate companies during the 2016-2020 period. Sampling using purposive sampling method. The analytical tools used in this research are multiple linear analysis and Moderated Regression Analysis (MRA). Based on the results of the study, it shows that operating capacity has a positive effect on financial distress, intellectual capital has a negative effect on financial distress, profitability can moderate the effect of operating capacity on financial distress and profitability cannot moderate the influence of intellectual capital on financial distress.
Cognitive development in school performance in high school students
Authors: Chávez-Mendoza María Virginia , Alcívar-Cedeño Monserrate Dalila del Rocío
Published: July 2022

The objective of this research is to determine the cognitive development in the school performance of the students of the upper basic of the fiscal educational institution "Santa Rita", of the Chone canton in the period 2021-2022. The applied methodology had an exploratory and descriptive quantitative-qualitative approach, taking the position of several authors and the opinion of directors and teachers of higher basic education; since they are the ones who guided the teaching-learning process of the students under their charge through various disciplines. When investigating teachers based on the survey carried out, it is evident that most of the respondents have followed the regulations and guidelines issued by the Ministry of Education through the districts with teaching through interdisciplinary projects and the implementation of a curriculum prioritized by the crisis experienced in the pandemic by Covid 19. The result was that teachers assumed the responsibility of bringing knowledge, but also becoming aware of the circumstances and motivation was permanently delivered so that students will take the right direction, being mostly the architects of their learning such as self-discipline, responsibility, and care.
An Examination of Organizational Crisis Communication Strategy
Authors: Barclay Lizabeth A. , Thor Jennifer Cordon
Published: July 2022

This paper examines Michigan State University’s (MSU) official communications related to the Nassar sexual assault case. Because of the public nature and media centric focus of the Nassar case, this set of communications offers a rare opportunity to investigate the role of a smoldering organizational crisis communication strategy. This study contributes to the crisis communication strategy literature by using directed content analysis. Unlike previous crisis communication research, the current study uses data posted over a four-year period. Results indicated that over time, in spite of a variety of strategic communication tactics that the number of university donors decreased while student applications remained relatively stable. Using the results, an extended model of strategic crisis communication is presented to guide future research.
Women’s Presence on Corporate Boards: Addressing Director Selection, Participation, and Gender Bias
Authors: Young Angela M.
Published: July 2022

Gender diversity of corporate boards is gaining attention as a global issue, but women’s presence on boards remains low despite increased numbers of qualified women. Most research compares gender diversity of boards and firm performance or corporate social responsibility compliance and reporting, ethics compliance, or other decisions impacting firm risk and success. Despite positive outcomes associated with gender diverse boards, few women directors are appointed, and progress is slow. Quotas and incentives have been considered and implemented in several countries to develop board gender diversity, but without enforced quotas, minimal change is seen. When women are appointed, they are often not present in sufficient number to impact board gender diversity. A direct focus on gender bias in director selection and board member participation is needed to provide a deeper understanding of women’s presence on boards.
Inducing and Recording Acute Stress Responses on a Large Scale With the Digital Stress Test (DST): Development and Evaluation Study
Authors: Norden Matthias , Hofmann Amin Gerard , Meier Martin , Balzer Felix , Wolf Oliver T , Böttinger Erwin , Drimalla Hanna
Published: July 2022

Background

Valuable insights into the pathophysiology and consequences of acute psychosocial stress have been gained using standardized stress induction experiments. However, most protocols are limited to laboratory settings, are labor-intensive, and cannot be scaled to larger cohorts or transferred to daily life scenarios.

Objective

We aimed to provide a scalable digital tool that enables the standardized induction and recording of acute stress responses in outside-the-laboratory settings without any experimenter contact.

Methods

On the basis of well-described stress protocols, we developed the Digital Stress Test (DST) and evaluated its feasibility and stress induction potential in a large web-based study. A total of 284 participants completed either the DST (n=103; 52/103, 50.5% women; mean age 31.34, SD 9.48 years) or an adapted control version (n=181; 96/181, 53% women; mean age 31.51, SD 11.18 years) with their smartphones via a web application. We compared their affective responses using the international Positive and Negative Affect Schedule Short Form before and after stress induction. In addition, we assessed the participants’ stress-related feelings indicated in visual analogue scales before, during, and after the procedure, and further analyzed the implemented stress-inducing elements. Finally, we compared the DST participants’ stress reactivity with the results obtained in a classic stress test paradigm using data previously collected in 4 independent Trier Social Stress Test studies including 122 participants overall.

Results

Participants in the DST manifested significantly higher perceived stress indexes than the Control-DST participants at all measurements after the baseline (P<.001). Furthermore, the effect size of the increase in DST participants’ negative affect (d=0.427) lay within the range of effect sizes for the increase in negative affect in the previously conducted Trier Social Stress Test experiments (0.281-1.015).

Conclusions

We present evidence that a digital stress paradigm administered by smartphone can be used for standardized stress induction and multimodal data collection on a large scale. Further development of the DST prototype and a subsequent validation study including physiological markers are outlined.
Assessing Social Media Data as a Resource for Firearm Research: Analysis of Tweets Pertaining to Firearm Deaths
Authors: Singh Lisa , Gresenz Carole Roan , Wang Yanchen , Hu Sonya
Published: July 2022

Background

Historic constraints on research dollars and reliable information have limited firearm research. At the same time, interest in the power and potential of social media analytics, particularly in health contexts, has surged.

Objective

The aim of this study is to contribute toward the goal of establishing a foundation for how social media data may best be used, alone or in conjunction with other data resources, to improve the information base for firearm research.

Methods

We examined the value of social media data for estimating a firearm outcome for which robust benchmark data exist—specifically, firearm mortality, which is captured in the National Vital Statistics System (NVSS). We hand curated tweet data from the Twitter application programming interface spanning January 1, 2017, to December 31, 2018. We developed machine learning classifiers to identify tweets that pertain to firearm deaths and develop estimates of the volume of Twitter firearm discussion by month. We compared within-state variation over time in the volume of tweets pertaining to firearm deaths with within-state trends in NVSS-based estimates of firearm fatalities using Pearson linear correlations.

Results

The correlation between the monthly number of firearm fatalities measured by the NVSS and the monthly volume of tweets pertaining to firearm deaths was weak (median 0.081) and highly dispersed across states (range –0.31 to 0.535). The median correlation between month-to-month changes in firearm fatalities in the NVSS and firearm deaths discussed in tweets was moderate (median 0.30) and exhibited less dispersion among states (range –0.06 to 0.69).

Conclusions

Our findings suggest that Twitter data may hold value for tracking dynamics in firearm-related outcomes, particularly for relatively populous cities that are identifiable through location mentions in tweet content. The data are likely to be particularly valuable for understanding firearm outcomes not currently measured, not measured well, or not measurable through other available means. This research provides an important building block for future work that continues to develop the usefulness of social media data for firearm research.
Perceptions of Oral Nicotine Pouches on Reddit: Observational Study (Preprint)
Authors: Shao Yihan , Zou Jonathan , Xie Zidian , Mayne Rachel Grana , Ossip Deborah J , Rahman Irfan , McIntosh Scott , Li Dongmei
Published: July 2022

BACKGROUND

Oral nicotine pouches are a new form of tobacco-free nicotine products launched in recent years with a variety of flavors.

OBJECTIVE

This study aims to examine the public perceptions and discussions of oral nicotine pouches on Reddit, a popular social media platform for sharing user experiences.

METHODS

Between February 15, 2019, and February 12, 2021, a total of 2410 Reddit posts related to oral nicotine pouches were obtained over a 2-year period. After the removal of unrelated or commercial posts, 653 Reddit posts related to oral nicotine pouches remained. Topics and sentiments related to oral nicotine pouches on Reddit were hand coded.

RESULTS

The number of Reddit posts related to oral nicotine pouches increased during the study period. Content analysis showed that the most popular topic was “sharing product information and user experience” (366/653, 56%), in which sharing oral nicotine pouch products and user experiences were dominant. The next popular topic was “asking product-related questions” (product properties and product recommendations; 115/653, 17.6%), followed by “quitting nicotine products” such as vaping or smoking through use of oral nicotine pouches or quitting the oral nicotine pouches themselves (83/653, 12.7%) and “discussing oral nicotine pouch–related health” symptoms or concerns related to oral nicotine pouches (74/653, 11.3%). The least popular topic was “legality and permissions” related to oral nicotine pouches (15/653, 2.3%). In addition, a greater number of Reddit posts described positive attitudes compared to negative attitudes toward oral nicotine pouches (354/653, 54.2% vs 101/653, 15.5%; P<.001).

CONCLUSIONS

Reddit posts overall had a positive attitude toward oral nicotine pouches and users were actively sharing product and user experiences. Our study provides the first insight on up-to-date oral nicotine pouch discussions on social media.
Title: Blockchain Solutions for Managing Value-based Care: A Systematic Review (Preprint)
Authors: Holan Cole , Yan Derek , Khurshid Anjum
Published: July 2022

BACKGROUND

Value in healthcare has been defined as the health outcome per dollar of cost expended. However, the barrier to achieving the implementation of value-based health care (VBHC) lies in its many structural, technological, and economic challenges. Six steps have been identified by the early proponents of VBHC to move towards a high-value healthcare delivery system: organizing integrated practice units (IPUs), measuring cost/outcomes for patients, moving to bundled payments, integrating care delivery, expanding care services, and enabling a suitable technology platform. Blockchain, given its unique properties of decentralization, data storage, and user platform, shows promise as a technology that may help implement the steps to achieve VBHC. In this paper we turn to the literature surrounding blockchain technology to highlight and summarize existing and theoretical use cases that address one or more of these steps.

OBJECTIVE

Describe blockchain-based solutions that can be utilized for implementing steps to achieve value-based health care.

METHODS

Utilizing PRISMA methodology, a systematic review of blockchain articles pertaining to value-based care delivery using PubMed, IEEE Xplore, and Business Source Complete was performed from March and April of 2022 with no limitations on publication date. Articles included must 1) pertain to blockchain in healthcare and 2) include either an existing or theoretical/future use case for blockchain in implementing one of the six steps to achieve value-based healthcare as defined by Teisberg and Porter.

RESULTS

A total of 357 articles were identified from all three databases (87 from PubMed, 259 from IEEE Xplore, and 11 from Business Source Complete) with 288 articles excluded. There were 69 articles included in the review with all of them (69) discussing interoperability and 18 discussing scalability. No articles were found pertaining to the other 4 steps to achieve value-based health defined by Teisberg and Porter.

CONCLUSIONS

This study examined the literature for articles pertaining to blockchain and value-based healthcare. While blockchain is early in its development as a technology, there is a significant body of research discussing how blockchain’s interoperability can be leveraged to improve value-based delivery systems. There is still a lack of research evaluating other core areas of a value-based delivery system, particularly relating to integrated practice units, measuring costs and outcomes, and bundled payments.
Assessment of medical device software supporting healthcare services for chronic patients: experience from a tertiary hospital (Preprint)
Authors: Baltaxe Erik , Hsieh Moriah , Roca Josep , Cano Isaac
Published: July 2022

BACKGROUND

Innovative digital health tools are increasingly being evaluated and, in some instances, integrated at scale into health systems. However, applicability of assessment methodologies in real-life scenarios to demonstrate value generation and, consequently, foster sustainable adoption of digitally enabled health services still shows bottlenecks.

OBJECTIVE

To build on lessons learnt in the process of pre-market assessment of four digital health interventions piloted at Hospital Clinic of Barcelona (HCB), as well as in the analysis of current Medical Device Software (MDSW) regulations and post-market surveillance in EU and USA, to generate recommendations towards large scale adoption.

METHODS

Four digital health interventions using prototypes were piloted at HCB. Co-creation and quality improvement methodologies were used to consolidate a pragmatic evaluation method to assess end-user’s (both patients and healthcare professionals) perceived usability and satisfaction by means of the System Usability Scale and the Net Promoter Score, including general questions about satisfaction. Analyses of both Medical Software Device (MDSW) regulations and post-market surveillance in EU and USA (2017-2021) were done. Finally, an overarching analysis on lessons learnt was conducted considering four domains (technical, clinical, usability and cost), as well as differentiating among three different eHealth strategies (Telehealth, Integrated Care and Digital Therapeutics).

RESULTS

Among the participant stakeholders, the system usability scale was consistently higher in case of patients (78, 67, 56 and 76) than in health professionals (52, 43, and 54), and, in general terms, patients would recommend more the use of the supporting digital health tools (NPS of -3, 31, -21 and 31) than professionals (NPS of -67, 1 and -80). The overarching analysis resulted in pragmatic recommendations for the digital health evaluation domains and the eHealth strategies considered.

CONCLUSIONS

Lessons learnt on digitalisation of health resulted in practical recommendations that could contribute to fostering future deployment experiences.
Are we ready to "see" mental illness? Ethical implications concerning the use of language analysis technologies in diagnosing and forecasting psychiatric disorders (Preprint)
Authors: Loch Alexandre , Lopes-Rocha Ana Caroline , Ara Anderson , Gondim João Medrado , Cecchi Guillermo , Corcoran Cheryl Mary , Mota Natália Bezerra , Argolo Felipe
Published: July 2022

UNSTRUCTURED

Recent developments in artificial intelligence technologies have come up to a point where machine learning algorithms can infer mental status based on someone's photos and texts posted on social media. More than that, these algorithms are able to predict, with a reasonable degree of accuracy, future mental illness. They potentially represent an important advance in mental healthcare for preventive and early diagnosis initiatives, and for aiding professionals in the follow-up and prognosing of their patients. However, important issues call for major caution in the use of such technologies, namely privacy and the stigma related to mental disorders. In this article we discuss the bioethical implications of using such technologies to diagnose and to predict future mental illness, given the current scenario of swiftly growing technologies to analyze human language, and the online availability of personal information given by social media. We also suggest future directions to be taken to minimize the misuse of such important technologies.
Management of Intermittent Hemodialysis in the Critically Ill Patient
Authors: Chan Ryan J. , Helmeczi Wryan , Canney Mark , Clark Edward G.
Published: July 2022

Intermittent hemodialysis remains a cornerstone of extracorporeal KRT in the intensive care unit, either as a first-line therapy for AKI or a second-line therapy when patients transition from a continuous or prolonged intermittent therapy. Intermittent hemodialysis is usually provided 3 days per week in this setting on the basis that no clinical benefits have been demonstrated with more frequent hemodialysis. This should not detract from the importance of continually assessing and refining the hemodialysis prescription (including the need for extra treatments) according to dynamic changes in extracellular volume and other parameters, and ensuring that an adequate dose of hemodialysis is being delivered to the patient. Compared with other KRT modalities, the cardinal challenge encountered during intermittent hemodialysis is hemodynamic instability. This phenomenon occurs when reductions in intravascular volume, as a consequence of ultrafiltration and/or osmotic shifts, outpace compensatory plasma refilling from the extravascular space. Myocardial stunning, triggered by intermittent hemodialysis, and independent of ultrafiltration, may also contribute. The hemodynamic effect of intermittent hemodialysis is likely magnified in patients who are critically ill due to an inability to mount sufficient compensatory physiologic responses in the context of multiorgan dysfunction. Of the many interventions that have undergone testing to mitigate hemodynamic instability related to KRT, the best evidence exists for cooling the dialysate and raising the dialysate sodium concentration. Unfortunately, the evidence supporting routine use of these and other interventions is weak owing to poor study quality and limited sample sizes. Intermittent hemodialysis will continue to be an important and commonly used KRT modality for AKI in patients with critical illness, especially in jurisdictions where resources are limited. There is an urgent need to harmonize the definition of hemodynamic instability related to KRT in clinical trials and robustly test strategies to combat it in this vulnerable patient population.
The Efficiency of Aloe vera Gel Extract in Inhibiting the Growth of Aspergillus flavus Fungus Associated with Imported and Domestic Rice Grains in Iraq and its Ability to Reduce Aflatoxin B1 Production
Authors:   Ministry of Agriculture Agricultural Directorate of Karbala, Iraq, Abbass AlHamiri Kamal Abdulkarim , Hussein Halima Z. ,   College of Agricultural Engineering Sciences, University of Baghdad, Iraq
Published: July 2022

Al-Hamiri, K.A.A. and H.Z. Hussein. 2022. The Efficiency of Aloe vera Gel Extract in Inhibiting the Growth of Aspergillus flavus Fungus Associated with Imported and Domestic Rice Grains in Iraq and its Ability to Reduce Aflatoxin B1 Production. Arab Journal of Plant Protection, 40(2): 164-168. https://doi.org/10.22268/AJPP-040.2.164168 The study aimed to evaluate the efficiency of different concentrations of Aloe vera gel extract in inhibiting the growth of A. flavus on potato dextrose agar (PDA) culture medium, as well as evaluating the efficiency of the extract in inhibiting the growth of A. flavus and reducing Aflatoxin B1 (AFB1) production in rice grains. The results obtained showed that the concentrations 1, 2, 3, and 4% of Aloe vera gel extract caused variable inhibition rates of A. flavus growth on PDA culture medium; of 97, 100, 5.88 and 17.64%, respectively. The best concentration from the laboratory experiment, (2%) was used, to evaluate its efficiency in inhibiting the growth of A. flavus on rice grains during storage and reduced AFB1 production by 86.5%, from 21.2 ppb in the control to 2.86 ppb in the treatment using HPLC high- performance liquid chromatography. The same treatment reduced AFB1 concentration in rice grains contaminated with AFB1 by 74.7%, from 22.88 ppb in control to 5.78 ppb in the extract treatment. Keywords: A. flavus, mycotoxins, aflatoxin B1, Aloe vera, food, feed, toxicity
Morphological and Molecular Characterization of Fusarium chlamydosporum, F. brachygibbosum and F. flocciferum Associated with Crown and Root Rot of Wheat
Authors:   Damascus University, Second Faculty of Agriculture, Sweida, Syria, Zidan L. , Jawdat D ,   Atomic Energy Commission, Department of Molecular Biology and Biotechnology, P. O. 6091, Damascus, Syria, Naffaa Walid ,   Damascus University, Faculty of Agriculture, Department of Plant Protection, 30621 Damascus, Syria
Published: July 2022

Zidan, L., D. Jawdat and W. Naffaa. 2022. Morphological and Molecular Characterization of Fusarium chlamydosporum, F. brachygibbosum and F. flocciferum Associated with Crown and Root Rot of Wheat. Arab Journal of Plant Protection, 40(2): 175-181. https://doi.org/10.22268/AJPP-040.2.175181 Fusarium is one of the most important genera of fungi, causing plant, animal, and human diseases and produces mycotoxins. A total of 105 isolates of Fusarium spp. were recovered from crowns and roots of wheat plants, showing typical symptoms, collected from four Syrian provinces during 2017-2018. In previous studies, seventeen Fusarium species associated with crown and root rot (FCR) on wheat were identified in Syria. However, the identity of some isolates has not been resolved. The current study was carried out to identify and characterize through morphological approaches and sequencing a partial translation elongation factor 1-alpha (TEF1-α) gene in three Fusarium species F. chlamydosporum Wollenweber & Reinking, F. brachygibbosum Padwick and F. flocciferum Corda associated with FCR, isolated and identified for the first time in Syria. The present study will provide detailed cultural and morphological characteristics of the three species, scarcely described in literature. Keywords: First record, morphological descriptions, TEF1-α gene, Syria.
Профессиональная деятельность И.В. Жолтовского в годы Второй мировой войны. К творческой биографии мастера
Authors: Печёнкин Илья , Шурыгина Ольга
Published: July 2022

В статье изложены и проанализированы данные о творче­ской деятельности И.В. Жолтовского (1867-1959) в период 1939-1945 годов. Исторический контекст, сформированный Второй мировой войной, обусловил обращение архитекторов к новому кругу задач, существенно отличавшихся от тех, что были актуальны в предыдущем десятилетии. Жолтовский, утвердившийся в довоенные годы в статусе главного отече­ственного эксперта по классической традиции и ведущего архитектурного педагога, не стал исключением. Он плотно сотрудничал со структурами Наркомата обороны СССР и Наркомата внутренних дел СССР, осуществлявшими проекти­рование и строительство для оборонной промышленности, включая Атомный проект. Стилевые характеристики про­ектов, созданных Жолтовским в рассматриваемый период, не могут быть определены через привычные для описания его творчества мирного времени отсылки к итальянскому Ренессансу, палладианству и т.п. - это совершенно особая.
* Исследование выполнено за счёт средств государственной программы Российской Федерации «Научно-технологическое развитие Российской Федерации» на 2021–2030 годы в рамках Плана фундаментальных научных исследований РААСН и Минстроя России на 2022 год, тема № 1.1.1.1. «Архитектурное творчество на перекрёстках культур Востока и Запада. От поздней античности до Нового времени».
Dissecting G-Quadruplexes and I-Motifs in Cis-Regulatory Regions of Mammals and Plants
Authors: Renuka Vemparala , Rajesh Yella Venkata
Published: July 2022

The evolutionary constraints of a gene consist of cis-gene regulatory regions, such as promoters and enhancers, which contribute to the regulation of gene expression. Several genomic and computational studies stated the roles of G-quadruplex and i-motif structures in vital cellular processes like transcription, translation, gene regulation, etc. The formation of these non-B DNA structures is supported by the occurrence of unique repeated sequences. However, many studies lean toward understanding the role of the G-quadruplex, and only recent studies indicated i-motif significance. In this study, we attempted to dissect the enrichment of G-quadruplex and i-motifs in promoter regions of mammals and plants. To this end, we employed the genomic sequences encompassing -500 to +500 region relative to the gene start positions in mammals and plants retrieved from the UCSC browser and Plant Genome database (PlantGDB). We computed the putative G-quadruplexes and i-motifs with well-recognized regular expression sequence patterns. We observed that G-quadruplex motifs showed preponderance in mammals, algal species, namely, green algae, and Chlamydomonas when compared to plants. Contrastingly i-motifs are enriched in both monocot and dicot plants compared to G-quadruplex motifs. The comparative examinations in this study revamp our understanding of the two quadruplex structures and their emerging functional roles in complex eukaryotes.
Knowledge, Attitude and Practice of Weight Reduction Among Overweight and Obese Adolescent Students
Authors: Sanjay Bhujbal Akshata , Baby Biji , T. Poovishnu Devi
Published: July 2022

Overweight/obesity is usually associated with some complications that adversely affect the health of the individuals. Due to an increase in sedentary life style, increased consumption of junk food and lack of physical exertion, there's an impact on their body image by increase in weight leading to overweight and obesity. This study assessed the level of knowledge, attitude and practice of weight reduction among overweight and obese adolescent students in Karad community. This cross-sectional study included 94 overweight and obese individuals in which, 55 were females and 39 were males. A self-administered questionnaire was used to collect data on sociodemographic characteristics, knowledge, attitude and practice of weight reduction among overweight and obese adolescent students. A total of 94 participants were included in this study, among which 57% participants had good knowledge on weight reduction, the attitude regarding weight reduction in our study was observed that 52% of participants had a positive attitude. The level of practice was less compared to knowledge and attitude, 19% participants actually participated in weight reduction programs. The level of good knowledge and positive attitude is not enough to prevent complications of overweight and obesity. More efforts should be taken to increase the practice of overweight and obese adolescent students by active participation in weight reduction program. The study is important to highlight the importance of weight reduction programs in overweight and obese adolescent students. It also indicates the severity of ill effects of overweight and obesity. As many adolescents are facing the problems of overweight and obesity, it is important to make awareness of weight reduction programs. The result of this study indicates that there is a need to implicate the weight reduction programs in overweight and obese adolescent students.
Antagonistic Potential Marine Bacteria Against Luminescent Vibriosis Pathogen Vibrio Harveyi Obtained from Shrimp Farm, Marakkanam, Tamil Nadu, India
Authors: Sakthivel Murugan , Jayanthi Jayaprakash , Gurunathan Ragunathan Manickavalli
Published: July 2022

Aquaculture farming practices in India and other countries are facing a lot of economic loss due to luminescent vibriosis.Ecological and cost-effective practices may have been warranted to combat this tricky disease. A total of 57 morphologicallydifferent marine bacterial isolates were isolated from samples collected at different aquaculture ponds in Marakkanam, Tamil Nadu,India and screened for their antibacterial potential against luminescent vibriosis causing bacteria Vibrio harveyi. All marine bacterialisolates were screened for antibacterial activity by cross streak method against two different isolates V. harveyi GNC01 and V.harveyi GNC03. Secondary screening of antagonistic isolates by dual culture plate method and agar well diffusion method leads tothe identification of antagonistic isolates. The antagonistic isolates were characterized by molecular taxonomy (16S rRNA genesequence) and phylogeny and identified as Neobacillus niacini (GNC04), Bacillus halotolerans (GNC06), Bacillus amyloliquefaciens(GNC16), Lacticaseibacillus paracasei (GNC23) and Lactiplantibacillus plantarum (GNC24). The minimum inhibitory concentrationof all these selected bacterial crude secondary metabolites against both Vibrio harveyi isolates GNC01 and GNC03 ranged between0.062 to 0.50 mg/mL. The GC-MS profiling of the ethyl acetate extract revealed the presence of antibacterial compounds such asOxime-,methoxy-phenyl (26.63%), 2,3-Dihydroxybutane (9.52%), Phosphite, menthyldimethyl- (2.83%), Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- (2.07%). The results of this study suggest that Lactiplantibacillus plantarum (GNC24) is apotential source for antagonistic secondary metabolites against luminescent vibriosis causing bacteria Vibrio harveyi. Further,biological control formulation with these isolates and their metabolites will be a reward for the integrated pest management inthe aquaculture sector.
Effectiveness of Virtual Reality Training for Hand Dexterity in Parkinson’s Subjects
Authors: Vaishnavi G , Saranya P , Senthil Nathan C.V , Kamatchi K , Pavithralotchani V , Deepa I , Pradeepa K , Sumitha A
Published: July 2022

Our aim of the study is to determine the effectiveness of virtual reality training for hand dexterity in Parkinson’s subjects. Parkinson’s disease is one of the most common neurodegenerative disorders. Parkinson’s disease (PD) includes multi level impairments in functional, motor activities which leads to worsening the patient's physical and psychological disabilities, more than 10 million people worldwide are living with Parkinson’s disease. Hand dexterity is as essential as walking ability or postural stabilization or maintaining independence and quality of daily living activities. A comparative study was done with 20 samples. The study was conducted in ACS Medical College and Hospitals, Physiotherapy OP, Krrish Physiotherapy clinic. The duration of treatment was 8 weeks. Both male and female individuals with Parkinson’s disease in concern about hand dexterity difficulty, between the age of 50-55 were included. PD with other neurologic diagnosis, Cognitive impairments, Vision impairments, Uncooperative medication, Cardiac problems, uncooperative were excluded. The outcome measure tools are Box and Block test (BBT) and Chedoke Arm and Hand activities Inventory(CAHAI-13). Based on inclusion and exclusion criteria and outcome measures, 20 subjects were divided into two groups. Group A (10 subjects) were treated with virtual reality training using Leap Motion Controller for 4 days a week for 2 months and Group B (10 subjects) were treated with conventional physiotherapy for 4 days a week for 2 months. On comparing the mean values of Group A& Group B by using CAHAI-13 and BBT score, the Virtual reality Training seems to have shown better results in manual dexterity in Parkinson’s subjects. People with mild to severe disability Parkinson’s have reduced dexterity and problems with hand function
Therapeutic Evaluation of Vrikshamla (Garcinia Cambogia) in the Management of Sthoulya (Obesity) W.S.R Dyslipidemia a Case Report
Authors: Mathapati Ishwarayya S , R Manu , P Punith , R H Jyothi
Published: July 2022

In recent years’ obesity which is one among the metabolic syndrome is a leading health problem which can be corelated to dyslipidaemia – “prayaha snehat meda pravaradhyet”, implying the factor that excessive sneha(oleation) is responsible for excessive production of meda dhatu (fatty tissue) and such excessively accumulated meda dhatu (fatty tissue) causing avarodha (obliteration) of other dhatu(tissues) leading to medoroga(dyslipidaemia) or else it is taken as sthoulya (Obesity). Obesity is always associated with dyslipidaemia and other such metabolic disorders. There is an exact explanation to this pathology in Ayurveda and our acharyas have mentioned proper shamana aushadi (alleviating therapy) which can tackle both obesity as well as dyslipidaemia together. Hence our aim for this case report is to find out the efficacy of Vrikshamla (Garcinia Cambogia) in the management of sthoulya (Obesity) and dyslipidaemia and objectives that were assessed are body weight, BMI, body circumference and lipid profile of the patient. A 32-year-old male patient was a known case of obesity and diagnosed with dyslipidaemia based on the laboratory investigation. Patient was not a known case of any other metabolic disorders and administered with shamana aushadi (alleviating therapy) vrikshamla 2gms per day for 60 days. Patient was assessed before and after treatment with the subjective and objective parameters were taken up for the study which showed the drastic improvement in weight reduction of 6kgs and BMI was reduced to 29.2 from 32.2 and shows better results in lipid profile as well. Drug Vrikshamala (extract) which has amla(sour), katu(pungent) and madhura(sweet) rasa(taste) with laghu(lightness) and usna(hot) guna(properties) in predominance and having the kapha medohara property helps in agnisam dhukshana (stimulate digestive power) and does the ama sanchaya, clears the srotorodha (obstruction of channels) was profoundly successful in reducing weight, BMI and lipid profile of a patient. Thus vrikshamala shows a better efficacy against sthoulya(obesity) and in dyslipidemia.
Chemotherapeutic Drugs Induced Symptom Distress Among Pediatric Cancer Patients
Authors: Jeayareka J , Raman K , Singh Lokesh Kumar , Jagzape Tushar Bharat
Published: July 2022

Pediatric cancer treatment includes several chemotherapeutic drugs in combination with adequate supportive management. Symptom distress among pediatric cancer patients on chemotherapy interferes with treatment compliance. Poorly managed distress leads to the decline of the child’s ability physically and mentally and deteriorates the quality of life. This paper shows the effect of chemotherapeutic drugs induced symptom distress, and the effect of early diagnosis in the management and improvement in the quality of life of pediatric cancer patients. For this study60, pediatric cancer patients aged between 10 to 18 years including both the gender were chosen using purposive sampling. Using the M.D. Anderson symptom inventory the symptom distress experienced by the pediatric cancer patients was assessed. The present quantitative cross-sectional study was conducted at the AIIMS, Raipur. The data were analyzed using the descriptive Statistical Package for Social Science Version 16 (SPSS 16). The categorical data was analyzed using chi –square test and the cut off p-value at ≤0.05. Among, the pediatric cancer patients 77.65% (SD 18.85) were experiencing severe symptom distress when on chemotherapy. Whereas 53.65 %( SD 9.63). of them experiencing the symptoms those disturb their activities of daily living in the last twenty four hours at p-value of ≤0.05. There is a need for an adequate support system from the school, social relationship, siblings, and health care team for improving the quality of life of pediatric cancer patients. Animal facilitated therapy, artistics expressions, medical play, procedural education, well organized home bound or school programs help the pediatric cancer patients to manage the symptom distress, thereby improving the treatment outcome. The specific supportive chemotherapeutic management is essential for the pediatric cancer patient’s treatment compliance and prognosis. The health care team, especially nurses play a vital role in early detection of chemotherapeutic agents induced symptom distress and its management.
Bioanalytical Method Development and Validation of Selinexor in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry
Authors: Yaswanth Murthaeti , Sreekanth M , Valli Syed Sadaq , Narayana D Venkata , Sreedhar V , Venkataramana Kanuma , Pravallika K E
Published: July 2022

A simple, rapid, precise, sensitive, and reproducible reverse-phase high-performance liquid chromatography (RPHPLC) LCMS/MS method has been developed for the bioanalytical method for Selinexor with D6-Selinexor as Internal Standard in the pharmaceutical dosage form. Chromatographic separation of Selinexor was achieved on Waters Alliance-e2695, by using X-Bridge phenyl, 150x4.6mm, 3.5μm column, and the mobile phase containing 0.1% Formic acid & Acetonitrile in the ratio of 80:20% v/v. The flow rate was 1.0 ml/min; detection was carried out by absorption at 225nm using a photodiode array detector at ambient temperature. The method was validated to fulfill International Conference on Harmonization (ICH) requirements and this validation included specificity, selectivity, matrix effect, linearity, the limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy. The proposed method was Bio-analytical validated according to USFDA guidelines. This method was found to be a very simple, economical, suitable, precise, accurate, and stable method for pharmacokinetic analysis of Selinexor and study of its stability. The calibration curve was linear over the concentration range from 0 to 40 ng/ml, and the lower limit of detection of 12.5 ng/ml. The accuracy and precision of the method were within the acceptable limit of ±20% at the lower limit of quantitation and ±15% at other concentrations. Selinexor was unstable at room temperature it showed more than 25% loss after 24 h. While, Selinexor is very stable at refrigerator 40C auto-sampler, freeze/thaw cycles, and 30 days storage in a freezer at 35 ± 20C. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs.
Response Surface Methodology Based Development and Quantification of Celecoxib and Amlodipine Using RP-HPLC.
Authors: B. Shah Darshil , H. Patel Bhavesh , S. Shah Jignesh
Published: July 2022

A novel RP-HPLC method for estimation of Amlodipine Besylate and Celecoxib using an experimental design approach employing response surface technique was developed and validated using a C18 column and its application in marketed formulation. A multivariate optimization of experimental conditions was carried out using experimental design employing organic content in the mobile phase, pH, and flow rate of the mobile phase as three independent variables. The aim of the study was to apply response surface methodology and to study the effect of the independent variables on separation and estimation of both drugs by RP-HPLC method using faced central composite (FCC) experimental design which is novel in this area of research. Optimization of retention time of the last eluting peak and peak symmetry was performed using Derringer’s desirability function in which potassium dihydrogen phosphate buffer with pH 4. 45, 25 mM, 0.5% Triethylamine and acetonitrile in an isocratic proportion of 70:30% v/v and 1.2 ml/min flow rate was found to be the predicted optimum condition. DoE approach based RP-HPLC method development using response surface methodology led to efficient separation with a lower retention time of eluted peaks below 7 min indicating novelty of research. A linear response was observed over the concentration range of 40-240 μg/mL for Celecoxib and 1-6 μg/ mL for Amlodipine Besylate. Limit of detection (LOD) and limit of quantitation (LOQ) for Celecoxib were found to be 0.02 μg/mL and 0.08μg/mL, and for Amlodipine Besylate were 0.0053 μg/mL and 0.0162 μg/mL, respectively. The method was successfully validated in accordance with ICH guideline acceptance criteria for linearity, accuracy, precision, specificity, robustness.
Efficacy of Carob Leaves and Pods in Reducing Cyclophosphamide-Induced Toxicity on Bone Marrow and Peripheral Blood Leukocytes
Authors: Y. Abajy Mohammad , Nayal Ream , Alqubaji Mahassen , Abdrabbo Yara
Published: July 2022

Cyclophosphamide is one of the chemotherapeutics for cancer, however this drug has severe side effects in multiple organs and is a well-known mutagenic agent. In this work the protective activity of antioxidant-rich Carob (Ceratonia siliqua L.) pods and leaves extracts against cyclophosphamide induced genotoxicity was studied. Safety of these extracts was evaluated in acute and subacute toxicity tests at a dose of 1500 mg/kg/day in albino rats. Both extracts exhibited no mortality or significant changes in rats' general behavior. Moreover, organ weights and blood chemistry revealed no toxic effects of these extracts (p>0.05).The anti-mutagenic potential of carob pods and leaves extracts were evaluated using in vivo bone marrow chromosomal aberration and micronucleus assays and in vitro comet assay for peripheral blood white cells. Results showed that rats that were treated with cyclophosphamide along with carob pods had a significant decrease in chromosomal aberrations when compared with cyclophosphamide-treated rats (p<0.05). Pods extract also showed significant reduction in cyclophosphamide-induced DNA migration (p<0.05). There were also highly statistically significant differences in the means of micronuclei (MNs) in immature red blood cells between cyclophosphamide group and carob leaves group (p<0.01).It could be concluded that Carob extracts had protective effect against cyclophosphamide- induced genotoxicity and may be considered as anti-mutagenic agent after conducting more studies.