bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding populist politics in Turkey: a hegemonic depth approach
Authors: Yalvaç Faruk , Joseph Jonathan
Published: August 2019

The aim of this article is to understand populism as a hegemonic project involving a struggle for power between different social forces. We take a critical realist approach in defining populism. This implies several things. We develop a new approach to understanding populist politics by taking neither a purely discursive (Laclau), nor a solely structural (Poulantzas), but a critical realist approach and analysing the three-way relationship between structural conditions, agency, and institutional framework. Second, it implies that populist politics is composed of complex and often contradictory dynamics and emergent features involving mainly domestic but also international processes. We develop this through a combination of three concepts – passive revolution, hegemonic depth, and partial hegemony. These indicate how a hegemonic project is situated in deeper social relations and how hegemonic leadership responds to this. We take the policies of AKP government in Turkey as a case in populist hegemonic project. We demonstrate that AKP has followed different hegemonic projects during its rule changing from an initial majoritarian populist politics to one of neoliberal authoritarian populism as it has consolidated its hegemonic depth. These different populist projects involve alternative visions of Turkey but are nevertheless all compatible with a global neoliberal agenda.
Moderated Mobilization: A New Model of Enterprise-level Collective Bargaining in South China
Authors: Luo Siqi , Yang Tao
Published: August 2019

In response to a series of strikes in south China in 2010, a new model of collective bargaining has emerged, featuring what this article describes as “moderated mobilization.” Distinct from what is typically known as China's quadripartite industrial relations system, whereby workers are separated from the party-state, official trade unions and employers, this model shows workers and enterprise-level trade unions in collaboration with one another. According to our observations from 2012 to 2017, some enterprise unions have successfully mobilized workers throughout the collective bargaining process. These unions are democratically elected by workers and are relatively independent from the official authorities. At the same time, they have “moderated” such mobilization particularly to reduce labour militancy, given the political and institutional constraints within which they must work. The implication of this new model is significant. Although it might be far from solving the quadripartite dilemma, it has signalled an increase in local initiatives among enterprise unions – a previously neglected but pragmatically favourable channel for workers.
Longitudinal examination of pathways to peer problems in middle childhood: A siblings-reared-apart design
Authors: Leve Leslie D. , Griffin Amanda M. , Natsuaki Misaki N. , Harold Gordon T. , Neiderhiser Jenae M. , Ganiban Jody M. , Shaw Daniel S. , Reiss David
Published: August 2019

To advance research from Dishion and others on associations between parenting and peer problems across childhood, we used a sample of 177 sibling pairs reared apart since birth (because of adoption of one of the siblings) to examine associations between parental hostility and children's peer problems when children were ages 7 and 9.5 years (n = 329 children). We extended conventional cross-lagged parent–peer models by incorporating child inhibitory control as an additional predictor and examining genetic contributions via birth mother psychopathology. Path models indicated a cross-lagged association from parental hostility to later peer problems. When child inhibitory control was included, birth mother internalizing symptoms were associated with poorer child inhibitory control, which was associated with more parental hostility and peer problems. The cross-lagged paths from parental hostility to peer problems were no longer significant in the full model. Multigroup analyses revealed that the path from birth mother internalizing symptoms to child inhibitory control was significantly higher for birth parent–reared children, indicating the possible contribution of passive gene–environment correlation to this association. Exploratory analyses suggested that each child's unique rearing context contributed to his or her inhibitory control and peer behavior. Implications for the development of evidence-based interventions are discussed.
Early life predictors of attention deficit/hyperactivity disorder symptomatology profiles from early through middle childhood
Authors: Willoughby Michael T. , Williams Jason , Mills-Koonce W. Roger , Blair Clancy B.
Published: August 2019

This study used repeated measures data to identify developmental profiles of elevated risk for ADHD (i.e., six or more inattentive and/or hyperactive-impulsive symptoms), with an interest in the age at which ADHD risk first emerged. Risk factors that were measured across the first 3 years of life were used to predict profile membership. Participants included 1,173 children who were drawn from the Family Life Project, an ongoing longitudinal study of children's development in low-income, nonmetropolitan communities. Four heuristic profiles of ADHD risk were identified. Approximately two thirds of children never exhibited elevated risk for ADHD. The remaining children were characterized by early childhood onset and persistent risk (5%), early childhood limited risk (10%), and middle childhood onset risk (19%). Pregnancy and delivery complications and harsh-intrusive caregiving behaviors operated as general risk for all ADHD profiles. Parental history of ADHD was uniquely predictive of early onset and persistent ADHD risk, and low primary caregiver education was uniquely predictive of early childhood limited ADHD risk. Results are discussed with respect to how changes to the age of onset criterion for ADHD in DSM5 may affect etiological research and the need for developmental models of ADHD that inform ADHD symptom persistence and desistance.
Nest site selection and its implications for conservation of the endangered Oriental Stork Ciconia boyciana in Yellow River Delta, China
Authors: CHENG LEI , ZHOU LIZHI , WU LIXIN , FENG GUANGHAI
Published: August 2019

Avian nest site selection is crucial to breeding birds especially for large endangered waterbirds. We investigated the population dynamics, nests and breeding habitat selection, offspring numbers and reproductive success rate of Oriental Stork Ciconia boyciana from February to June 2017 in Yellow River Delta National Nature Reserve (YRD NNR), Shandong, China. We measured the characteristic variables of 32 control plots by random selection and 62 nest plots, including 40 nests on power poles, 14 nests on artificial poles and eight nests on pylon poles. We used conditional logistic regression and model-averaging to quantify data and model characteristic variables affecting nest site selection. Our results showed that human disturbance was the main negative factor affecting Oriental Stork’s selection of power pole nests. Meanwhile, distance from highways was the most important variable for the selection of artificial nests. Distance from sightseeing sites was the main explanatory variable that contributed to the selection of pylon nests. Based on our findings, we recommend promoting conservation of this species by reducing human disturbance, such as forbidding use of car horns in nest site areas and providing enough suitable nest sites.
Factors affecting safe and healthy diet in older adults in Italy: results of a preliminary study performed in a community-dwelling sample
Authors: Laurenti P , De Meo C , Sacchini D , Spagnolo AG , Moro D , Varacca A , Landi F , Manes-Gravina E , Sgadari A , Bernabei R , Barbara A , Ricciardi W , de Waure C
Published: August 2019

Objective:

To investigate, through a questionnaire, older adults’ demographic and socio-economic characteristics, knowledge, attitudes and practices in terms of food safety and healthy diet; and to develop dietary and hygiene indices able to represent participants’ nutritional and food safety behaviour, exploring their association with demographic and socio-economic factors.

Design:

One-year cross-sectional study.

Setting:

Gemelli Teaching Hospital (Rome, Italy).

Participants:

People aged ≥65 years, Italian speaking, accessing the Centre of Ageing Medicine.

Results:

Mean age of the sample was 74 (sd 7·7) years. Subjective perception of a safe diet was high: 64·2 % of respondents believed they have a balanced diet. Interviewees got informed about proper nutrition mainly from television, magazines, newspapers, Internet (29·9 %) and from health professionals (34·8 %) such as dietitians, whereas 15·4 % from general practitioners. Regarding food safety, 33·8 % of participants reported to consume expired food, even more than once per month; between 80 and 90 % of participants reported to follow food safety practices during preparation and cooking, even though 49·3 % defrosted food at room temperature. Calculated dietary and hygiene indices showed that the elderly participants were far from having optimal nutritional and food safety behaviours.

Conclusions:

These results suggest it is necessary to increase the awareness of older adults in the matter of healthy diet and food safety. Specific and targeted educational interventions for the elderly and their caregivers could improve the adoption of recommended food safety practices and safe nutritional behaviours among older adults.
Geographical proximity and the transmission of drug abuse among siblings: evaluating a contagion model in a Swedish National Sample
Authors: Kendler Kenneth S. , Ohlsson Henrik , Edwards Alexis C. , Sundquist Jan , Sundquist Kristina
Published: August 2019

Aims

Can drug abuse (DA) be transmitted psychologically between adult siblings consistent with a social contagion model?

Methods

We followed Swedish sibling pairs born in 1932–1990 until one of them, sibling1 (S1), had a first DA registration. We then examined, using Cox regression, the hazard rate for a first registration for DA in sibling2 (S2) within 3 years of a first DA registration in S1 as a function of their geographical proximity. We examined 153 294 informative pairs. To control for familial confounding, we repeated these analyses in sibships containing multiple pairs, comparing risk in different siblings with their proximity to S1. DA was recorded in medical, criminal or pharmacy registries.

Results

The best-fit model predicted risk for DA in S2 as a function of the log of kilometres between S1 and S2 with parameter estimates (±95% confidence intervals) of 0.94 (0.92; 0.95). Prediction of DA included effects of cohabitation and an interaction of proximity and time since S1 registration with stronger effects of proximity early in the follow-up period. Proximity effects were stronger for smaller S1–S2 age differences and for same- v. opposite-sex pairs. Sibship analyses confirmed sibling-pair results.

Conclusions

Consistent with a social contagion model, the probability of transmission of a first registration for DA in sibling pairs is related to their geographical proximity and similarity in age and sex. Such effects for DA are time-dependent and include cohabitation effects. These results illustrate the complexity of the familial aggregation of DA and support efforts to reduce their contagious spread within families in adulthood.
Cover crops and topography differentially influence weeds at a watershed scale
Authors: Singh Gurbir , Kaur Gurpreet , Williard Karl W. , Nelson Kelly A. , Schoonover Jon E.
Published: August 2019

Cover crops (CCs) play an important role in integrated weed management. Data necessary to evaluate the role of CCs in weed management at the watershed scale with topographic positions are lacking. We evaluated the effects of cereal rye and hairy vetch CCs on weed suppression at different topographic positions (shoulder, backslope, and footslope) at a watershed scale. Watersheds with a CC treatment followed a crop rotation of corn–cereal rye–soybean–hairy vetch, whereas watersheds without a CC (no-CC) had a crop rotation of corn–winter fallow–soybean–winter fallow. A negative relationship was present between CCs and weed biomass at the shoulder, backslope, and footslope topographic landscape positions, with R2 values of 0.40, 0.48, and 0.50, respectively. In 2016, a cereal rye CC reduced weed biomass 46% to 50% at footslope and shoulder positions compared to no CC. In 2018, a cereal rye CC reduced weed biomass between 52% and 85% at all topographic positions in CC treatment watersheds compared to no-CC watersheds. Hairy vetch in 2017 reduced weed biomass 62% to 72% at footslope and shoulder topographic positions in CC watersheds compared to no-CC. The C:N ratio of weed biomass in CC treatment watersheds was generally higher compared to watersheds without CCs. In this study, several significant interactions were found between the topographic positions and CC treatments. Cover crop–induced weed suppression at different topographic positions can lead to developing better site-specific weed control strategies. Therefore, CC interactions with topography, weed germination potential, and the role of soil moisture at the watershed scale should be further evaluated.
Mechanisms of Methane Hydrate Formation in Geological Systems
Authors: You K. , Flemings P.B. , Malinverno A. , Collett T.S. , Darnell K.
Published: August 2019

Natural gas hydrate is ice‐like mixture of gas (mostly methane) and water that is widely found in sediments along the world's continental margins and within and beneath permafrost and glaciers in a near‐surface depth interval where the pressure is sufficiently high and temperature sufficiently low for gas hydrate to be stable. We categorize the myriad of geological gas hydrate deposits into five characteristic types. We then review the multiple quantitative models that have proposed to describe the genesis of these deposits and describe how each may have formed. We emphasize the importance of coupling multiphase flow (free gas and liquid water) and multicomponent reactive transport with geological history to describe the dynamical processes of gas hydrate formation and evolution in geological systems. A better insight into the kinetics of methane formation from microbial biogenesis and the processes of multiphase flow at the pore scale will advance our knowledge of how these systems form. By understanding the generation and evolution of gas hydrate through time, we will better decipher the role of gas hydrate in the carbon cycle, its potential to contribute to climate change and geohazards, and how to design optimal strategies for gas production from hydrate reservoirs.
Attribution of the East Asian Winter Temperature Trends During 1979–2018: Role of External Forcing and Internal Variability
Authors: Gong Hainan , Wang Lin , Chen Wen , Wu Renguang
Published: August 2019

Attributing the surface air temperature (SAT) trends at regional scale to internally generated and externally forced components is a major challenge. Based on the observations and ensemble simulations of climate models, we apply a “dynamical adjustment” methodology to estimate quantitatively the contribution of the internal and forced dynamic and thermodynamic components to the East Asian winter SAT trends during 1979–2018. The observed winter SAT trends are strongly influenced by internal variability, especially in the northern East Asia (NEA). The internal component of observed SAT trends is largely dynamically induced, whereas the forced component is controlled by thermodynamics. The internally generated variability offsets the forced warming by more than 70% in the NEA, leading to a weak observed warming in the NEA. In contrast, internal contributions are small in the southern East Asia. The internally generated SAT changes in the NEA are closely tied to the multidecadal changes of Arctic Oscillation.
Testing Contrasting Models of the Formation of the Upper Yellow River Using Heavy‐Mineral Data From the Yinchuan Basin Drill Cores
Authors: Wang Zhao , Nie Junsheng , Wang Junping , Zhang Haobo , Peng Wenbin , Garzanti Eduardo , Hu Xiaofei , Stevens Thomas , Pfaff Katharina , Pan Baotian
Published: August 2019

The upper Yellow River drains the central and northeastern Tibetan Plateau. Understanding the origin of this river is essential for unraveling the interplay between fluvial incision, basement uplift, and climate change. However, the formation age of the upper Yellow River is highly debated, with estimates ranging from Eocene to late Pleistocene. In order to clarify the history of the upper Yellow River, we present a heavy‐mineral dataset from drill core in the Yinchuan Basin, a depositional sink at the end of the upper Yellow River course. Our results reveal that the drainage area of the upper Yellow River (i.e., northeastern Tibetan Plateau) has been serving as a major sediment source region for the Yinchuan Basin since at least 3.3 Ma. A late Pleistocene formation of the upper Yellow River, and erosion of neighboring deserts or bounding mountains as an explanation for thick sediment accumulations in this area, is thus ruled out.
A Model Investigation of Aerosol‐Induced Changes in the East Asian Winter Monsoon
Authors: Liu Zhen , Ming Yi , Wang Lin , Bollasina Massimo , Luo Ming , Lau Ngar‐Cheung , Yim Steve Hung‐Lam
Published: August 2019

The response of the East Asian winter monsoon (EAWM) circulation to aerosols is studied using a coupled atmosphere‐slab ocean general circulation model. In the extratropics, the aerosol‐induced cooling in the midlatitudes leads to an intensified subtropical jet stream, a deepened East Asian trough, and thus an enhanced EAWM. In the tropics, the local Hadley circulation shifts southward to compensate for the interhemispheric asymmetry in aerosol radiative cooling. Anomalous subsidence at around 10°N leads to a salient anticyclone to the southwest of the Philippines. The associated southwesterlies advect abundant moisture to South China, resulting in local precipitation increases and suggesting a weaker EAWM. The EAWM response to aerosol forcing is thus driven by a competition between tropical and extratropical mechanisms, which has important implications for the future monsoon evolution, as aerosol changes may follow different regional‐dependent trajectories.
Marine Aerosol Production via Detrainment of Bubble Plumes Generated in Natural Seawater With a Forced‐Air Venturi
Authors: Frossard Amanda A. , Long Michael S. , Keene William C. , Duplessis Patrick , Kinsey Joanna D. , Maben John R. , Kieber David J. , Chang Rachel Y.‐W. , Beaupré Steven R. , Cohen Ronald C. , Lu Xi , Bisgrove John , Zhu Yuting
Published: August 2019

During September–October 2016, a marine aerosol generator configured with forced‐air Venturis was deployed at two biologically productive and two oligotrophic regions of the western North Atlantic Ocean to investigate factors that modulate primary marine aerosol (PMA) production. The generator produced representative bubble size distributions with Hinze scales (0.32 to 0.95 mm radii) and void fractions (0.011 to 0.019 Lair Lsw‐1) that overlapped those of plumes produced in the surface ocean by breaking wind waves. Hinze scales and void fractions of bubble plumes varied among seawater hydrographic regions, whereas corresponding peaks and widths of bubble size distributions did not, suggesting that variability in seawater surfactants drove variability in plume dynamics. Peaks in size‐resolved number production efficiencies for model PMA (mPMA) emitted via bubble bursting in the generator were within a narrow range (0.059 to 0.069 μm geometric mean diameter) over wide ranges in subsurface bubble characteristics, suggesting that subsurface bubble size distributions were not the primary controlling factors as was suggested by previous work. Total mass production efficiencies for mPMA decreased with increasing air detrainment rates, supporting the hypothesis that surface bubble rafts attenuate mPMA mass production. Total mass and Na+ production efficiencies for mPMA from biologically productive seawater were significantly greater than those from oligotrophic seawater. Corresponding mPMA number distributions peaked at smaller sizes during daytime, suggesting that short‐lived surfactants of biological and/or photochemical origin modulated diel variability in marine aerosol production.
Carbon Chemistry of Intact Versus Chronically Drained Peatlands in the Southeastern USA
Authors: Stricker C. A. , Drexler J. Z. , Thorn K. A. , Duberstein J. A. , Rossman S.
Published: August 2019

The Great Dismal Swamp National Wildlife Refuge (GDS) is a large temperate swamp in Virginia/North Carolina, USA with peat soils historically resistant to microbial decomposition. However, this peatland has been subject to ~200 years of disturbance during which extensive drainage, fire suppression, and widespread logging have increased decomposition and dramatically decreased the distribution of Atlantic white cedar (AWC). The purpose of this study was to determine the impact of long‐term drainage and AWC loss on the carbon chemistry of GDS peats. Peat cores were collected from three drained GDS vegetation communities (pocosin, AWC, and red maple‐black gum) and compared to cores collected from an intact, undrained AWC peatland at the Alligator River National Wildlife Refuge (AR) in North Carolina. The AR peats had higher lignin content in the deeper peat intervals, and lignin content and percent organic carbon were largely invariant with depth compared to the GDS peats. The concentrations of syringyl group phenols were greater in the surface layers of GDS peats, likely reflecting the selective removal of AWC and transition from gymnosperms to angiosperms. Acid to aldehyde ratios for vanillyl and syringyl group phenols indicated that the GDS peats were more decomposed, particularly at depth, and that this occurred under aerobic conditions. Moreover, solid‐state 13C NMR confirmed a coincident loss of carbohydrates and increase in recalcitrant by‐products of carbohydrate degradation with depth. These data indicate that long‐term drainage has accelerated the decomposition of peat at the GDS, reducing the capacity and stability of the carbon sink.
Foraminiferal Ultrastructure: A perspective From Fluorescent and Fluorogenic Probes
Authors: Frontalini F. , Losada M. T. , Toyofuku T. , Tyszka J. , Goleń J. , de Nooijer L. , Canonico B. , Cesarini E. , Nagai Y. , Bickmeyer U. , Ikuta T. , Tsubaki R. , Besteiro Rodriguez C. , Al‐Enezi E. , Papa S. , Coccioni R. , Bijma J. , Bernhard J. M.
Published: August 2019

Microscopy techniques have been widely applied to observe cellular ultrastructure. Most of these techniques, such as transmission electron microscopy, produce high‐resolution images, but they may require extensive preparation, hampering their application for in vivo examination. Other approaches, such as fluorescent and fluorogenic probes, can be applied not only to fixed specimens but also to living cells when the probes are nontoxic. Fluorescence‐based methods, which are generally relatively easy to use, allow visual and (semi)quantitative studies of the ultrastructural organization and processes of the cell under natural as well as manipulated conditions. To date, there are relatively few published studies on the nearly ubiquitous marine protistan group Foraminifera that have used fluorescent and fluorogenic probes, despite their huge potential. The aim of the present contribution is to document the feasible application of a wide array of these probes to foraminiferal biology. More specifically, we applied fluorescence‐based probes to study esterase activity, cell viability, calcium signaling, pH variation, reactive oxygen species, neutral and polar lipids, lipid droplets, cytoskeleton structures, Golgi complex, acidic vesicles, nuclei, and mitochondria in selected foraminiferal species.
The impact of Semaphorin 4C/Plexin-B2 signaling on fear memory via remodeling of neuronal and synaptic morphology
Authors: Simonetti Manuela , Paldy Eszter , Njoo Christian , Bali Kiran Kumar , Worzfeld Thomas , Pitzer Claudia , Kuner Thomas , Offermanns Stefan , Mauceri Daniela , Kuner Rohini
Published: August 2019

Aberrant fear is a cornerstone of several psychiatric disorders. Consequently, there is large interest in elucidation of signaling mechanisms that link extracellular cues to changes in neuronal function and structure in brain pathways that are important in the generation and maintenance of fear memory and its behavioral expression. Members of the Plexin-B family of receptors for class 4 semaphorins play important roles in developmental plasticity of neurons, and their expression persists in some areas of the adult nervous system. Here, we aimed to elucidate the role of Semaphorin 4C (Sema4C) and its cognate receptor, Plexin-B2, in the expression of contextual and cued fear memory, setting a mechanistic focus on structural plasticity and exploration of contributing signaling pathways. We observed that Plexin-B2 and Sema4C are expressed in forebrain areas related to fear memory, such as the anterior cingulate cortex, amygdala and the hippocampus, and their expression is regulated by aversive stimuli that induce fear memory. By generating forebrain-specific Plexin-B2 knockout mice and analyzing fear-related behaviors, we demonstrate that Sema4C-PlexinB2 signaling plays a crucial functional role in the recent and remote recall of fear memory. Detailed neuronal morphological analyses revealed that Sema4C-PlexinB2 signaling largely mediates fear-induced structural plasticity by enhancing dendritic ramifications and modulating synaptic density in the adult hippocampus. Analyses on signaling-related mutant mice showed that these functions are mediated by PlexinB2-dependent RhoA activation. These results deliver important insights into the mechanistic understanding of maladaptive plasticity in fear circuits and have implications for novel therapeutic strategies against fear-related disorders.
TRAIL-based gene delivery and therapeutic
strategies
Authors: Zhong Hui-hai , Wang Hui-yuan , Li Jian , Huang Yong-zhuo
Published: August 2019

TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), also
known as APO2L, belongs to the tumor necrosis factor family. By binding to the death
receptor 4 (DR4) or DR5, TRAIL induces apoptosis of tumor cells without causing side
toxicity in normal tissues. In recent years TRAIL-based therapy has attracted great
attention for its promise of serving as a cancer drug candidate. However, the
treatment efficacy of TRAIL protein was under expectation in the clinical trials
because of the short half-life and the resistance of cancer cells. TRAIL gene
transfection can produce a “bystander effect” of tumor cell killing and provide a
potential solution to TRAIL-based cancer therapy. In this review we focus on TRAIL
gene therapy and various design strategies of TRAIL DNA delivery including non-viral
vectors and cell-based TRAIL therapy. In order to sensitize the tumor cells to
TRAIL-induced apoptosis, combination therapy of TRAIL DNA with other drugs by the
codelivery methods for yielding a synergistic antitumor efficacy is summarized. The
opportunities and challenges of TRAIL-based gene delivery and therapy are
discussed.
Kinetic asymmetry allows macromolecular catalysts to drive an information ratchet
Authors: Astumian R. Dean
Published: August 2019

Molecular machines carry out their function by equilibrium mechanical motions in environments that are far from thermodynamic equilibrium. The mechanically equilibrated character of the trajectories of the macromolecule has allowed development of a powerful theoretical description, reminiscent of Onsager’s trajectory thermodynamics, that is based on the principle of microscopic reversibility. Unlike the situation at thermodynamic equilibrium, kinetic parameters play a dominant role in determining steady-state concentrations away from thermodynamic equilibrium, and kinetic asymmetry provides a mechanism by which chemical free-energy released by catalysis can drive directed motion, molecular adaptation, and self-assembly. Several examples drawn from the recent literature, including a catenane-based chemically driven molecular rotor and a synthetic molecular assembler or pump, are discussed.
Atomistic structures and dynamics of prenucleation clusters in MOF-2 and MOF-5 syntheses
Authors: Xing Junfei , Schweighauser Luca , Okada Satoshi , Harano Koji , Nakamura Eiichi
Published: August 2019

Chemical reactions in solution almost always take place via a series of minute intermediates that are often in rapid equilibrium with each other, and hence hardly characterizable at the level of atomistic molecular structures. We found that single-molecule atomic-resolution real-time electron microscopic (SMART-EM) video imaging provides a unique methodology for capturing and analyzing the minute reaction intermediates, as illustrated here for single prenucleation clusters (PNCs) in the reaction mixture of metal–organic frameworks (MOFs). Specifically, we found two different types of PNCs are involved in the formation of MOF-2 and MOF-5 from a mixture of zinc nitrate and benzene dicarboxylates at 95 °C and 120 °C, respectively. SMART-EM identified a small amount of 1-nm-sized cube and cube-like PNCs in the MOF-5 synthesis, but not in the MOF-2 synthesis. In the latter, we instead found only linear and square PNCs, suggesting that the MOF-2/-5 bifurcation takes place at the PNC stage.
Bacteria evoke alarm behaviour in zebrafish
Authors: Chia Joanne Shu Ming , Wall Elena S. , Wee Caroline Lei , Rowland Thomas A. J. , Cheng Ruey-Kuang , Cheow Kathleen , Guillemin Karen , Jesuthasan Suresh
Published: August 2019

When injured, fish release an alarm substance (Schreckstoff) that elicits fear in members of their shoal. Although Schreckstoff has been proposed to be produced by club cells in the skin, several observations indicate that these giant cells function primarily in immunity. Previous data indicate that the alarm substance can be isolated from mucus. Here we show that mucus, as well as bacteria, are transported from the external surface into club cells, by cytoplasmic transfer or invasion of cells, including neutrophils. The presence of bacteria inside club cells raises the possibility that the alarm substance may contain a bacterial component. Indeed, lysate from a zebrafishStaphylococcusisolate is sufficient to elicit alarm behaviour, acting in concert with a substance from fish. These results suggest that Schreckstoff, which allows one individual to unwittingly change the emotional state of the surrounding population, derives from two kingdoms and is associated with processes that protect the host from bacteria.
Self-organized formation of developing appendages from murine pluripotent stem cells
Authors: Mori Shunsuke , Sakakura Eriko , Tsunekawa Yuji , Hagiwara Masaya , Suzuki Takayuki , Eiraku Mototsugu
Published: August 2019

Limb development starts with the formation of limb buds (LBs), which consist of tissues from two different germ layers; the lateral plate mesoderm-derived mesenchyme and ectoderm-derived surface epithelium. Here, we report means for induction of an LB-like mesenchymal/epithelial complex tissues from murine pluripotent stem cells (PSCs) in vitro. The LB-like tissues selectively differentiate into forelimb- or hindlimb-type mesenchymes, depending on a concentration of retinoic acid. Comparative transcriptome analysis reveals that the LB-like tissues show similar gene expression pattern to that seen in LBs. We also show that manipulating BMP signaling enables us to induce a thickened epithelial structure similar to the apical ectodermal ridge. Finally, we demonstrate that the induced tissues can contribute to endogenous digit tissue after transplantation. This PSC technology offers a first step for creating an artificial limb bud in culture and might open the door to inducing other mesenchymal/epithelial complex tissues from PSCs.
Tumor exosome-based nanoparticles are efficient drug carriers for chemotherapy
Authors: Yong Tuying , Zhang Xiaoqiong , Bie Nana , Zhang Hongbo , Zhang Xuting , Li Fuying , Hakeem Abdul , Hu Jun , Gan Lu , Santos Hélder A. , Yang Xiangliang
Published: August 2019

Developing biomimetic nanoparticles without loss of the integrity of proteins remains a major challenge in cancer chemotherapy. Here, we develop a biocompatible tumor-cell-exocytosed exosome-biomimetic porous silicon nanoparticles (PSiNPs) as drug carrier for targeted cancer chemotherapy. Exosome-sheathed doxorubicin-loaded PSiNPs (DOX@E-PSiNPs), generated by exocytosis of the endocytosed DOX-loaded PSiNPs from tumor cells, exhibit enhanced tumor accumulation, extravasation from blood vessels and penetration into deep tumor parenchyma following intravenous administration. In addition, DOX@E-PSiNPs, regardless of their origin, possess significant cellular uptake and cytotoxicity in both bulk cancer cells and cancer stem cells (CSCs). These properties endow DOX@E-PSiNPs with great in vivo enrichment in total tumor cells and side population cells with features of CSCs, resulting in anticancer activity and CSCs reduction in subcutaneous, orthotopic and metastatic tumor models. These results provide a proof-of-concept for the use of exosome-biomimetic nanoparticles exocytosed from tumor cells as a promising drug carrier for efficient cancer chemotherapy.
Spectroscopic studies of atomic defects and bandgap renormalization in semiconducting monolayer transition metal dichalcogenides
Authors: Jeong Tae Young , Kim Hakseong , Choi Sang-Jun , Watanabe Kenji , Taniguchi Takashi , Yee Ki Ju , Kim Yong-Sung , Jung Suyong
Published: August 2019

Assessing atomic defect states and their ramifications on the electronic properties of two-dimensional van der Waals semiconducting transition metal dichalcogenides (SC-TMDs) is the primary task to expedite multi-disciplinary efforts in the promotion of next-generation electrical and optical device applications utilizing these low-dimensional materials. Here, with electron tunneling and optical spectroscopy measurements with density functional theory, we spectroscopically locate the mid-gap states from chalcogen-atom vacancies in four representative monolayer SC-TMDs—WS2, MoS2, WSe2, and MoSe2—, and carefully analyze the similarities and dissimilarities of the atomic defects in four distinctive materials regarding the physical origins of the missing chalcogen atoms and the implications to SC-mTMD properties. In addition, we address both quasiparticle and optical energy gaps of the SC-mTMD films and find out many-body interactions significantly enlarge the quasiparticle energy gaps and excitonic binding energies, when the semiconducting monolayers are encapsulated by non-interacting hexagonal boron nitride layers.
Modulation of actin polymerization affects nucleocytoplasmic transport in multiple forms of amyotrophic lateral sclerosis
Authors: Giampetruzzi Anthony , Danielson Eric W. , Gumina Valentina , Jeon Maryangel , Boopathy Sivakumar , Brown Robert H. , Ratti Antonia , Landers John E. , Fallini Claudia
Published: August 2019

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease of unknown etiology. Although defects in nucleocytoplasmic transport (NCT) may be central to the pathogenesis of ALS and other neurodegenerative diseases, the molecular mechanisms modulating the nuclear pore function are still largely unknown. Here we show that genetic and pharmacological modulation of actin polymerization disrupts nuclear pore integrity, nuclear import, and downstream pathways such as mRNA post-transcriptional regulation. Importantly, we demonstrate that modulation of actin homeostasis can rescue nuclear pore instability and dysfunction caused by mutant PFN1 as well as by C9ORF72 repeat expansion, the most common mutation in ALS patients. Collectively, our data link NCT defects to ALS-associated cellular pathology and propose the regulation of actin homeostasis as a novel therapeutic strategy for ALS and other neurodegenerative diseases.
Integrative transcriptome imputation reveals tissue-specific and shared biological mechanisms mediating susceptibility to complex traits
Authors: Zhang Wen , Voloudakis Georgios , Rajagopal Veera M. , Readhead Ben , Dudley Joel T. , Schadt Eric E. , Björkegren Johan L. M. , Kim Yungil , Fullard John F. , Hoffman Gabriel E. , Roussos Panos
Published: August 2019

Transcriptome-wide association studies integrate gene expression data with common risk variation to identify gene-trait associations. By incorporating epigenome data to estimate the functional importance of genetic variation on gene expression, we generate a small but significant improvement in the accuracy of transcriptome prediction and increase the power to detect significant expression-trait associations. Joint analysis of 14 large-scale transcriptome datasets and 58 traits identify 13,724 significant expression-trait associations that converge on biological processes and relevant phenotypes in human and mouse phenotype databases. We perform drug repurposing analysis and identify compounds that mimic, or reverse, trait-specific changes. We identify genes that exhibit agonistic pleiotropy for genetically correlated traits that converge on shared biological pathways and elucidate distinct processes in disease etiopathogenesis. Overall, this comprehensive analysis provides insight into the specificity and convergence of gene expression on susceptibility to complex traits.
Bio-mediated synthesis of 5-FU based nanoparticles employing orange fruit juice: a novel drug delivery system to treat skin fibrosarcoma in model animals
Authors: Mohammad Owais , Faisal Syed Mohd. , Ahmad Nadeem , Rauf Mohd. Ahmar , Umar Mohd Saad , Mujeeb Anzar Abdul , Pachauri Piyush , Ahmed Anees , Kashif Mohammad , Ajmal Mohammad , Zubair Swaleha
Published: August 2019

Nano-sized drug delivery systems (NDDS) have been widely exploited to achieve targeted delivery of pharmaco-materials. Traditional pharmaceutical approaches, implied in the synthesis of nano-formulations, are obscure owing to the incompatible physico-chemical properties of the core drug as well as some other factors crucial in development of NDDS. Infact, most of the existing methods used in development of NDDS rely on usage of additives or excipients, a special class of chemicals. Barring few exceptions, the usage of synthetic excipients ought to be curtailed because of several associated undesirable features. Such issues necessitate strategies that lead to development of the synthetic excipient free drug delivery system. Plant based extracts have great potential to induce synthesis of nano-sized particles. Considering this fact, here we propose a prototype employing orange fruit juice (OJ) to facilitate bio-mediated synthesis of nano-sized supra-molecular assemblies of 5-fluorouracil (5-FU), a potent anticancer drug. The as-synthesized 5-FU Nanoparticles (NPs) retained the anti-neoplastic efficacy of the parent compound and induced apoptosis in cancer cells. The novel 5-FU NPs formulation demonstrated enhanced efficacy against DMBA induced experimental fibrosarcoma in the mouse model when compared to the micro-sized crystals of parent 5-FU drug.
Hepsin-mediated Processing of Uromodulin is Crucial for Salt-sensitivity and Thick Ascending Limb Homeostasis
Authors: Olinger Eric , Lake Jennifer , Sheehan Susan , Schiano Guglielmo , Takata Tomoaki , Tokonami Natsuko , Debaix Huguette , Consolato Francesco , Rampoldi Luca , Korstanje Ron , Devuyst Olivier
Published: August 2019

Uromodulin is a zona pellucida-type protein essentially produced in the thick ascending limb (TAL) of the mammalian kidney. It is the most abundant protein in normal urine. Defective uromodulin processing is associated with various kidney disorders. The luminal release and subsequent polymerization of uromodulin depend on its cleavage mediated by the serine protease hepsin. The biological relevance of a proper cleavage of uromodulin remains unknown. Here we combinedin vivotesting on hepsin-deficient mice,ex vivoanalyses on isolated tubules andin vitrostudies on TAL cells to demonstrate that hepsin influence on uromodulin processing is an important modulator of salt transport via the sodium cotransporter NKCC2 in the TAL. At baseline, hepsin-deficient mice accumulate uromodulin, along with hyperactivated NKCC2, resulting in a positive sodium balance and a better adaptation to water deprivation. In conditions of high salt intake, defective uromodulin processing predisposes hepsin-deficient mice to a salt-wasting phenotype, with a decreased salt sensitivity. These modifications are associated with intracellular accumulation of uromodulin, endoplasmic reticulum-stress and signs of tubular damage. These studies expand the physiological role of hepsin and uromodulin and highlight the importance of hepsin-mediated processing of uromodulin for kidney tubule homeostasis and salt sensitivity.
Nanoscale Spatially Resolved Mapping of Uranium Enrichment
Authors: Kautz Elizabeth , Burkes Douglas , Joshi Vineet , Lavender Curt , Devaraj Arun
Published: August 2019

Spatially resolved analysis of uranium (U) isotopes in small volumes of actinide-bearing materials is critical for a variety of technical disciplines, including earth and planetary sciences, environmental monitoring, bioremediation, and the nuclear fuel cycle. However, achieving subnanometer-scale spatial resolution for such isotopic analysis is currently a challenge. By using atom probe tomography—a three-dimensional nanoscale characterisation technique—we demonstrate unprecedented nanoscale mapping of U isotopic enrichment with high sensitivity across various microstructural interfaces within small volumes (~100 nm3) of depleted and low-enriched U alloyed with 10 wt% molybdenum that has different nominal enrichments of 0.20 and 19.75% 235U, respectively. We map enrichment in various morphologies of a U carbide phase, the adjacent γ-UMo matrix, and across interfaces (e.g., carbide/matrix, grain boundary). Results indicate the U carbides were formed during casting, rather than retained from either highly enriched or depleted U feedstock materials. The approach presented here can be applied to study nanoscale variations of isotopic abundances in the broad class of actinide-bearing materials, providing unique insights into their origins and thermomechanical processing routes.
Heavy fermion quantum criticality at dilute carrier limit in CeNi2−δ(As1−xPx)2
Authors: Chen Jian , Wang Zhen , Li Yupeng , Feng Chunmu , Dai Jianhui , Xu Zhu’an , Si Qimiao
Published: August 2019

We study the quantum phase transitions in the nickel pnctides, CeNi2−δ(As1−xPx)2 (δ ≈ 0.07–0.22) polycrystalline samples. This series displays the distinct heavy fermion behavior in the rarely studied parameter regime of dilute carrier limit. We systematically investigate the magnetization, specific heat and electrical transport down to low temperatures. Upon increasing the P-content, the antiferromagnetic order of the Ce-4f moment is suppressed continuously and vanishes at xc ~ 0.55. At this doping, the temperature dependences of the specific heat and longitudinal resistivity display non-Fermi liquid behavior. Both the residual resistivity ρ0 and the Sommerfeld coefficient γ0 are sharply peaked around xc. When the P-content reaches close to 100%, we observe a clear low-temperature crossover into the Fermi liquid regime. In contrast to what happens in the parent compound x = 0.0 as a function of pressure, we find a surprising result that the non-Fermi liquid behavior persists over a nonzero range of doping concentration, xc < x < 0.9. In this doping range, at the lowest measured temperatures, the temperature dependence of the specific-heat coefficient is logarithmically divergent and that of the electrical resistivity is linear. We discuss the properties of CeNi2−δ(As1−xPx)2 in comparison with those of its 1111 counterpart, CeNi(As1−xPx)O. Our results indicate a non-Fermi liquid phase in the global phase diagram of heavy fermion metals.
Hyperbaric therapy provides no benefit for skeletal muscle and respiratory function and accelerates cardiac injury in mdx mice
Authors: Fischer Kaleb D. , Heitzman Jackie A. , Townsend DeWayne
Published: August 2019

Duchenne muscular dystrophy (DMD) is a uniformly fatal condition of striated muscle wasting resulting in premature death from respiratory and/or cardiac failure. Symptomatic therapy has prolonged survival by limiting deaths resulting from respiratory insufficiency, but there is currently no effective therapy for most patients with DMD. This grim prognosis has led patients and their families to seek unproven therapeutic approaches. One such approach is the use of hyperbaric therapies, which 14% of DMD patients self-report using. The primary goal of this study was to determine if intermittent hyperbaric exposure altered the muscle function of the mdx mouse, a genetic model of DMD. To do this, mdx mice were exposed to three daily 90-minute 1.3 atmosphere hyperbaric exposures for 4 weeks. Skeletal muscle, respiratory, and cardiac function were assessed in treated and untreated wild type and dystrophic mice. The results of these studies find that hyperbaric and hyperoxic approaches resulted in increased cardiac fibrosis in dystrophic mice and no beneficial effects on the functional parameters measured. These data suggest that these oxygen-based therapies are unlikely to provide therapeutic benefit to DMD patients.
Comparative transcriptome and metabolite profiling of four tissues from Alisma orientale (Sam.) Juzep reveals its inflorescence developmental and medicinal characteristics
Authors: Lin Wenjin , Sun Fengling , Zhang Yamin , Xu Xiaomei , Lu Xuehua , Li Lisha , Xu Rongqing
Published: August 2019

Alisma orientale (Sam.) Juzep (A. orientale) is an important medicinal plant in traditional Chinese medicine. In this study, de novo RNA-seq of A. orientale was performed based on the cDNA libraries from four different tissues, roots, leaves, scapes and inflorescences. A total of 41,685 unigenes were assembled, 25,024 unigene functional annotations were obtained by searching against the five public sequence databases, and 3,411 simple sequence repeats in A. orientale were reported for the first time. 15,402 differentially expressed genes were analysed. The morphological characteristics showed that compared to the other tissues, the leaves had more chlorophyll, the scapes had more vascular bundles, and the inflorescences contained more starch granules and protein. In addition, the metabolic profiles of eight kinds of alisols metabolite profiling, which were measured by ultra-Performance liquid chromatography-triple quadrupole-mass spectrometry showed that alisol B 23-acetate and alisol B were the major components of the four tissues at amounts of 0.068~0.350 mg/g and 0.046~0.587 mg/g, respectively. In addition, qRT-PCR validated that farnesyl pyrophosphate synthase and 3-hydroxy-3-methylglutaryl-CoA reductase should be considered the critical candidate genes involved in alisol biosynthesis. These transcriptome and metabolic profiles of A. orientale may help clarify the molecular mechanisms underlying the medicinal characteristics of A. orientale.
Hexagonal Close-packed Iron Hydride behind the Conventional Phase Diagram
Authors: Machida Akihiko , Saitoh Hiroyuki , Hattori Takanori , Sano-Furukawa Asami , Funakoshi Ken-ichi , Sato Toyoto , Orimo Shin-ichi , Aoki Katsutoshi
Published: August 2019

Hexagonal close-packed iron hydride, hcp FeHx, is absent from the conventional phase diagram of the Fe–H system, although hcp metallic Fe exists stably over extensive temperature (T) and pressure (P) conditions, including those corresponding to the Earth’s inner core. In situ X-ray and neutron diffraction measurements at temperatures ranging from 298 to 1073 K and H pressures ranging from 4 to 7 GPa revealed that the hcp hydride was formed for FeHx compositions when x < 0.6. Hydrogen atoms occupied the octahedral interstitial sites of the host metal lattice both partially and randomly. The hcp hydride exhibited a H-induced volume expansion of 2.48(5) Å3/H-atom, which was larger than that of the face-centered cubic (fcc) hydride. The hcp hydride showed an increase in x with T, whereas the fcc hydride showed a corresponding decrease. The present study provides guidance for further investigations of the Fe–H system over an extensive x–T–P region.