bluuebunny/crossref_metadata_2025 huggingface dataset:
Anticoncepción posevento obstétrico y embarazo subsecuente en adolescentes: perspectivas del personal de salud en México
Authors: Campero Lourdes , Romero Irma , Estrada Fátima , Hubert Celia , Heredia-Pi Ileana B , Villalobos-Hernández Aremis
Published: July 2022

Objetivo. Identificar cómo se implementa la estrategia de anticoncepción posevento obstétrico (APEO) y analizar las barreras, acciones y recomendaciones para prevenir embara­zos subsecuentes en adolescentes. Material y métodos. Estudio cualitativo a través de análisis de contenido de 21 en­trevistas semiestructuradas a personal de salud de instituciones de atención a población sin seguridad social de la Ciudad de México y Morelos. Resultados. La APEO se promueve du­rante el embarazo, parto, posaborto, puerperio y hasta 12 me­ses después del evento obstétrico. La consejería debe abordar, además de información técnica sobre métodos anticonceptivos, otras temáticas clave en relación con los derechos sexuales y reproductivos de los adolescentes. Se identifican barreras personales, institucionales y sociales para la implementación de la estrategia; el personal refiere acciones y recomendaciones específicas para su consolidación. Conclusión. La estrategia de APEO se debe fortalecer para garantizar la oferta de un servicio acorde con las necesidades de las mujeres menores de 20 años, así como diseñar intervenciones basadas en las buenas prácticas y recomendaciones de los prestadores de salud para superar las barreras y lograr periodos intergenésicos más allá de la adolescencia.
Respuesta a la crítica del artículo “Prevalencia mensual de trastorno de ansiedad generalizada durante la pandemia por Covid-19 en México”
Authors: Gaitán-Rossi Pablo , Pérez-Hernández Víctor , Vilar- Compte Mireya , Teruel-Belismelis Graciela
Published: July 2022

No disponible
Birth weight percentiles in Western-Mexico
Authors: Corona-Gutiérrez América Aime , Camarena-Pulido Eva Elizabet , Arellano-Cardenas Juan Pablo , Robledo-Aceves Mireya , Quezada-Salazar Claudia Angélica , Gómez-Ruíz Larissa María , Pérez-Ramírez Rene Oswaldo
Published: July 2022

Not available
Sentiment Analysis based on Feature Selection Classification model
Authors: D Elangovan , V Subedha
Published: July 2022

As the use of social media and other online forums for exchanging ideas and exchanging opinions has grown, so has the field of sentiment analysis. Many people use social media like Twitter and Facebook to share their thoughts and ideas with the rest of the world. Opinion mining or sentiment analysis focuses on classifying and predicting a target's opinion. Text documents or sentences can be categorized based on whether they represent a favorable or negative view on a certain subject. Text categorization may seem like an easy process compared to sentiment analysis, but numerous difficulties have prompted many studies into this area. The use of Firefly with Levy and Multilayer Perceptron approaches combined with a feature selection-based classification model to automate sentiment analysis has been proposed. Online product reviews can be improved by incorporating feature selection and classification into the SA model presented in this research. Feature extraction from online product evaluations was accomplished using the firefly (FF) algorithm, while sentiment classification was accomplished using a multi-layer perceptron (MLP). Four datasets are used in the experiment, and the outcomes are evaluated according to various criteria. As a result of these tests, it can be concluded that the FF-MLP model provided here has the best classification performance over the whole dataset tested.
A Four-Year Survey of Error Reports in a Radiotherapy Department
Authors: Esmati Ebrahim , Hasoomi Nafise , Khodadadi Asma , Andish Neda Maal , Ghalehtaki Reza , Kazemian Ali
Published: July 2022

Purpose: Patient safety is a fundamental concept in radiotherapy due to the delivery of large radiation doses in a single fraction. Radiotherapy is a highly complex procedure with many steps, which confers a high risk of errors. This study aimed to investigate radiotherapy errors and their effects on patients to provide a framework for increasing our knowledge of radiotherapy, quality of treatment and patient safety . Materials and Methods: The total number of error reports was 648 during four years of this survey (2017-2020), 51% of which were attributed to near-misses, and the patients were not affected by the errors. Also, 40% of the errors were incident errors, while the rest were non-conformity errors. Meanwhile, the number of treatment courses was 12,755, 32,118, 34,052, and 39,784 throughout the survey during 2017-2020, respectively. Results: The annual error ratio was 2.2 in 100 treatment courses. Use of a collaborative interprofessional approach led to a decrease in the incident errors by 20% and an increase in near-misses by 24% during the survey( P =.002). Conclusion: Our outcomes indicated that analyzing radiotherapy errors and their effects on patients can help radiotherapy teams, including radiation oncologists, physicists, and radiation therapists, to provide the best services for the patients; it also improves teamwork and increases the quality of treatment.
Physicochemical Properties of Imported and Locally Produced Honey did not translate into its Microbial Quality and Antibacterial Activity
Authors: Nzeh Joseph , Quansah Lydia , Dufailu Osman Adamu
Published: July 2022

Honey is often considered a good substitute for sugar. However, concerns about the poor quality of locally produced honey have necessitated the importation of honey to meet the domestic demand in spite of Ghana’s good climatic conditions suitable for honey production. Due to the increasing level of demand for honey both locally and internationally, we highlighted here the need for an assessment of the physicochemical, microbiological quality and antibiotics resistance of microbes isolated from the imported and locally produced honey vis-à-vis international standards. Thirty honey samples which comprise 7 imported and 23 locally produced were randomly sampled from retail outlets consisting of supermarkets, marts, shops, and open markets as well as from production sites within the northern region for physicochemical analysis, microbial analysis and antibiogram. Results of the physicochemical quality analysis showed that sampled honey (both imported and locally produced) was within acceptable set standards. However, microbial quality was poor as a high incidence of bacterial contamination and resistance was recorded in the study. The High incidence of bacterial contamination and antibiotic resistance recorded in this study gives an indication that all is not well in the honey industry and therefore the need for serious concern to avert possible health issues associated with the consumption of honey found within the region.
Prevention of DDoS Attack in Cloud Computing using Fuzzy Q – Learning Algorithm
Authors: Kumar Animesh , Dutta Sandip , Pranav Prashant
Published: July 2022

Cloud computing technology created a revolution in IT Industry that provides on-demand resources, flexibility, scalability, and lower maintenance to infrastructure costs. Distributed Denial of Service (DDoS) attack blocks the services by flooding high or low volume of malicious traffic to exhaust the servers, resources, etc of cloud. In today’s era, they are even difficult to detect because of low rate traffic and its hidden approach in cloud. The study of all DDoS attack with their possible solution is essential to protect cloud computing environment. In this paper, we have proposed a Fuzzy Q Learning algorithm and Chebyshev’s Inequality principle to counter the problem of DDoS attacks. The Proposed framework follows the inclusion of Chebyshew’s inequality for work load prediction in cloud in the analysis phase and fuzzy QLearning in planning phase .Experimental results proves that in our proposed FQBDDA model, prevent DDoS attack.
Addressing unavoidable climate change loss and damage: A case study from Fiji’s sugar industry
Authors: Nand Moleen , Bardsley Douglas K. , Suh Jungho
Published: July 2022

Climate change loss and damage (L&D) presents an existential threat to vulnerable countries. This case study describes an example of vulnerable communities facing challenges in minimising, averting, and addressing L&D. Through a grounded theoretical lens, qualitative research was used to gain in-depth insights into climate change L&D from cyclones in Fiji's sugar industry. In-depth semi-structured interviews (n=68) were conducted, in two Indo-Fijian sugarcane communities, Barotu and Toko settlements in Western Viti Levu, Fiji, and with key stakeholders from government ministries, academia, and climate change response services. Key findings from this research reveal that despite implementing climate change adaptation measures, Fiji’s sugar industry faces devastating L&D from regular, severe cyclones. Much of that climate change L&D is irreversible and could be categorised as unavoidable L&D, including loss of property, crops, and income. Non-economic loss and damage (NELD) was also documented in both field sites, such as losses of homes and places of worship, cascading and flow-on effects, and also the heightening of uncertainty, fear, and trauma. The systematic documentation of L&D within vulnerable communities would improve understanding of L&D, including NELD, and assist to facilitate the mobilisation of urgent support and action to address L&D in countries that lack the capacities to respond independently. Key policy interventions are recommended such as livelihood diversification, integration of disaster risk reduction and climate change adaptation, and the operationalisation of the Santiago Network for Loss and Damage.
Methylene blue dosing strategies in critically ill adults with shock – a retrospective cohort study
Authors: Sari-Yavuz Sibel , Heck-Swain Ka-Lin , Keller Marius , Magunia Harry , Feng You-Shan , Haeberle Helene A. , Wied Petra , Schlensak Christian , Rosenberger Peter , Koeppen Michael
Published: July 2022

Background: Shock increases mortality in the critically ill and the mainstay of therapy is the administration of vasopressor agents to achieve hemodynamic targets. In the past, studies have found that the NO-pathway antagonist methylene blue improves hemodynamics. However, the optimal dosing strategy remains elusive. Therefore, we investigated the hemodynamic and ICU outcome parameters of three different dosing strategies for methylene blue. Methods: We performed retrospective cohort study of patients in shock treated with methylene blue. Shock was defined as norepinephrine dose > 0.1 µg/kg/min and serum lactate level > 2 mmol/l at the start of methylene blue administration. Different demographic variables, ICU treatment, and outcome parameters were evaluated. To compare the differences in the administration of vasopressors or inotropes, the vasoactive inotropic score (VIS) was calculated at different time points after starting the administration of methylene blue. Response to methylene blue or mortality at 28 days were assessed. Results: 262 patients from July 2014 to October 2019 received methylene blue. 209 patients met the inclusion criteria. Three different dosing strategies were identified: bolus injection followed by continuous infusion (n=111), bolus injection only (no continuous infusion; n=59) or continuous infusion only (no bolus prior; n=39). The groups did not differ in demographics, ICU scoring system, or comorbidities. In all groups, VIS decreased over time, indicating improved hemodynamics. Cardiogenic shock and higher doses of norepinephrine increased the chance of responding to methylene blue, while bolus only decreased the chance of responding to methylene blue treatment. 28-day mortality increased with higher SAPSII scores and higher serum lactate levels, while bolus injection followed by continuous infusion decreased 28-day mortality. No severe side effects were notedConclusions: Methylene blue as a bolus injection followed by continuous infusion decreases 28-day mortality in patients with shock. Clinicians should consider the administration of methylene blue in patients with refractory shock, regardless of the cause.
Augmenting protein biofortification in maize using teosinte (Zea mays ssp. parviglumis)
Authors: Varalakshmi S , Singh Narendra Kumar , Pareek Navneet , Senthilkumar V
Published: July 2022

Maize underwent domestication from the wild species Teosinte (Zea mays ssp. parviglumis) in the Balsas River valley of Mexico. While domestication and selective breeding of maize lost major alleles for kernel size and composition, Teosintes have a huge repository of novel and diverse alleles for protein content; have doubled protein content and higher levels of methionine-rich zeins than cultivated maize. To address the issue of protein in maize, we developed and investigated BC1F2:3 population derived from crossing between maize inbred line CML-451 and teosinte (Zea mays ssp. parviglumis). The data on ear traits and protein content were recorded on 126 BC1F2:3 individual cobs and parental lines. The protein content of among the BC1F2:3 lines had highly significant variation which varied from 9.53–16.49% for MP51 and MP6 lines, respectively. The results stipulated that protein content of teosinte introgressed maize lines is improved by 41.42% over CML-451. The introgressed population also exhibited significant variation for all ear traits investigated. Principal component analysis and cluster analysis have applied for estimation of genetic diversity among introgressed lines. Proteins content, ear length (cm), ear width (cm), kernel rows/ear and test weight (g) are all significant contributors to maximum diversity among teosinte introgressed maize lines. Cluster analysis highlighted the diversity among lines based on protein content and test weight and grouped them into six clusters. Cluster II lines were characterized by higher protein content and test weight values and were considered for wider use in breeding programmes. The results of the investigation highlight the substantial increase in the protein content of the teosinte derived maize lines and therefore indicates critical role of Zea mays ssp. parviglumis in protein biofortification of maize kernels.
Effect of lunch with different calorie and nutrient balances on dinner-induced postprandial glucose variability
Authors: Kuwahara Mai , Kim Hyeon-Ki , Furutani Akiko , Mineshita Yui , Nakaoka Takashi , Shibata Shigenobu
Published: July 2022

Aim
This study aimed to examine the effect of lunches with different calorie intakes (Study 1) and nutrient balances (Study 2) on dinner-induced postprandial glucose fluctuation.
Methods
Energy trial (Study 1): Thirteen healthy young participants (n = 10 men, n = 3 women) were investigated to determine the effects of different caloric intakes at lunch on glucose level variability. The study comprised four trials (no lunch, low lunch, standard lunch, and high-energy lunch). Energy balance trial (Study 2): Fourteen healthy young adults (n = 8 men, n = 6 women) were investigated to determine the effect of different nutrient balances during lunch on glucose level variability. The study consisted of four trials (standard, protein-rich, fat-rich, and carbohydrate-rich). In Studies 1 and 2, each trial was spaced at least one day apart, and breakfast and dinner were tested as meals. The mealtimes for each trial were then aligned. Continuous glucose monitoring (CGM) was used to assess the blood glucose fluctuations.
Results
Study 1: The no-lunch and low-energy lunch trials had significantly higher values in the incremental area under the curve (iAUC) of postprandial blood glucose at dinner compared to the standard and high-energy lunch trials (P < 0.01, P < 0.05 vs none), (respectively, P < 0.01 vs low-energy trial). Study 2: A significantly higher postprandial blood glucose iAUC for dinner was found in the fat-rich trial than that in the protein-rich and standard trials, respectively (P < 0.05).
Conclusions
Our findings indicate that skipping lunch and low-calorie or high-lipid intake increased postprandial blood glucose levels after dinner.
Neural invasion is an independent prognostic factor in young and lymph node negative gastric cancer patients underwent curative gastrectomy
Authors: Wang Linjun , Xia Yiwen , Jiang Tianlu , Li Ying , Li Fengyuan , Wang Weizhi , Zhang Diancai , Xu Hao , Yang Li , Xu Zekuan
Published: July 2022

Background: The prognostic significance of neural invasion (NI) in gastric cancer (GC) has not been established. This study is to investigate the characteristic and prognostic value of NI in GC.Methods: 592 patients who had undergone gastrectomy for GC were retrospectively analyzed. NI was defined when cancer cells infiltrated into the perineurium or neural fascicles by hematoxylin and eosin staining of surgical specimens. NI and the other clinical factors were analyzed.Results: NI was detected in 270 of the 592 patients. NI was associated with tumor size, site, depth of invasion, lymph node metastasis, TNM stage, tumor differentiation, Lauren classification, and blood vessel invasion. NI was associated with the overall survival. Multivariate analysis indicated that NI was not an independent prognostic factor for total patients, while NI independently predicted prognosis for age<60 and lymph node metastasis negative patients by subgroup analysis. Concomitant existence of NI with tumor size ≥3cm, TNM stage III, or diffused Lauren classification independently predicted prognosis.Conclusions: The frequency of NI is high in GC patients and increases with disease progression. NI is related to poor survival in GC patients who underwent curative gastrectomy and provides independent prognostic value for young and lymph node metastasis negative patients.
Effects of dietary omega-3-polyunsaturated fatty acid in animal models of experimentally-induced granulomatous inflammation.
Authors: Omale Jamila Audu , Ezeanyika L. U. S. , C. Nwanguma B. , Omale A
Published: July 2022

Recent advances have shown how PUFA regulate molecular signaling, especially in the context of inflammation. Nutritional interventions with PUFA in experimental animal models have exhibited anti-inflammatory properties. In this study, chronic inflammation was assayed by Cotton-Pellet Induced Granuloma to evaluate the anti-inflammatory and anti-granuloma effects of Omega-3-polyunsaturated fatty acid using experimentally induced inflammatory models in rats. Obtained results indicates that omega-3 oil extract significantly (p < 0.05) reduced the granuloma tissue formation (58.36 ± 3.52, 65.40 ± 1.30, 57.78 ± 3.89, 50.93 ± 0.60 and 42.17 ± 0.70mg representing 33.78, 25.79, 34.44, 42.21 and 52.15% inhibition respectively for the proliferative phase) when compared to the control. This results showed that omega-3 oil extract studied, posses both anti-inflammatory and anti-granuloma properties
A case of infantile Pompe disease with intrauterine onset and literature review
Authors: Xi Hongmin , Li Xianghong , Ma Lili , Yin Xiangyun , Yang Ping , Zhang Lulu
Published: July 2022

Background: Pompe disease is a rare autosomal recessive disease.Acid alpha-glucosidase deficiency leads to glycogen storage in lysosomes, causing skeletal muscle, cardiac muscle and smooth muscle lesions.Pompe disease is progressive and the severity of the disease depends on the age of onset.The most severe form, known as classic infantile Pompe disease (IOPD), characterized by onset before 12 months.However, few cases of intrauterine onset have been reported.Case presentation: The proband was born at 40 weeks+3 days of gestational age and admitted to our hospital due to cardiac hypertrophy found in the uterus, shortness of breath and cyanosis after birth for 13 minutes. Physical examination at admission time showed poor response, pale skin, shortness of breath, low limb muscle tone and edema of both lower extremities. The heart sounds were weak without heart murmur. Echocardiography showed: left ventricular hypertrophy (9mm),right ventricular hypertrophy (5mm); The patient was given non-invasive ventilator-assisted respiration, fluid restriction, diuresis and metoprolol treatment. The child was diagnosed as infantile Pompe disease at 16 days with the acid alpha-glucosidase 0.31umol/l/h and the full penetrance gene test showing homozygous gene mutation c. 1844G>T(p.Gly615Val).The literature search retrieved a total of 3 articles and reported 5 Pompe disease infants with intrauterine onset. So there was a total of 6 cases,among whom,4 cases showing hypertrophic cardiomyopathy, 1 case with dilated cardiomyopathy, and 1 case had myocardial "mass". All cases had creatine kinase(CK) elevation, GAA decrease and gene mutation.2 cases needed ventilator assisted support and 4 cases were treated with enzyme replacement therapy(ERT), one of whom died at 14months; 2 patients without ERT treatment progressed rapidly, and our patient died at 7 months of age.Conclusion: Infants with intrauterine-onset Pompe disease mostly have early manifestations of heart disease. GAA determination and molecular genetic testing as soon as possible are helpful for parents to make early decision and early treatment.
LncRNA AOC4P impacts the differentiation of macrophages and T-lymphocyte by regulating the NF-κB pathways of KGN cells: potential pathogenesis of polycystic ovary syndrome
Authors: Wu Shujuan , Yang Zhe , He Shaojing , Han Lu , Zhou Mengqi , Yang Jing , Chen Jiao , Wu Gengxiang
Published: July 2022

Background
Polycystic ovary syndrome (PCOS) is a multifactorial endocrine disease, which is an important cause of female infertility worldwide. PCOS patients are in a state of chronic low-grade inflammation, and immune imbalance is considered as a potential cause of its pathogenesis.
Methods
The expression of AOC4P in PCOS and normal ovarian granulosa cells (GCs) was detected by real-time quantitative PCR. KGN cells were induced by dihydrotestosterone at 500 ng/ml to construct PCOS model. After lentivirus-infected, KGN cells were constructed with AOC4P overexpression cell lines, the proliferation and apoptosis levels of KGN cells in AOC4P and NC groups were detected. THP-1-derived macrophages and peripheral blood mononuclear cells (PBMC) were co-cultured with KGN cells for 48h respectively, and the differentiation of macrophages and CD4 + T cells were detected by flow cytometry.
Results
Decreased AOC4P expression was found in PCOS patients. After constructing PCOS cell model, we observed that overexpression of AOC4P promoted KGN cell proliferation and inhibited apoptosis. After co-culture with AOC4P overexpressed KGN cells, M1 macrophages decreased, M2 macrophages increased, Th1/Th2 ratio increased, and Treg cells increased. Finally, we found that AOC4P inhibited the activation of the nuclear factor κ B (NFκB) pathway in KGN cells.
Conclusions
In this study, we found that AOC4P regulated the NFκB signaling pathway by inhibiting the phosphorylation of P65, thereby affecting the proliferation and apoptosis of GCs, altering the differentiation of macrophages and T cells, and contributing to the pathogenesis of PCOS.
Summary of Research Progress on Separation and Extraction of Valuable Metals from Bayer Red Mud
Authors: Wang Kun , Dou Zhihe , Liu Yan , Li Xiaofei , Lv Guozhi , Zhang Ting-an
Published: July 2022

Bayer red mud is a strong alkaline solid waste discharged during alumina production. Due to large emissions and strong alkalinity, red mud is now mostly dammed or buried, which not only occupies huge land but also contaminates the surrounding ecosystem, causing the risk of collapse and landslide. In addition to its overall utilization in building materials, agriculture, the environment, and the chemical industry, red mud also contains valuable metals such as sodium, aluminum, iron, titanium, and scandium and is considered to be an important secondary resource. In this paper, the physicochemical properties and hazards of red mud are first introduced, and then the overall utilization of red mud is summarized. Then, the latest research progress on the separation and extraction of valuable metals from red mud is reviewed in detail, and a new comprehensive utilization method is proposed and analyzed. This paper also provides suggestions for the future development direction of the comprehensive utilization technology of red mud.
Diversity of the endophytic and rhizospheric fungal communities in Scrophularia ningpoensis roots and its correlation with active constituent contents
Authors: Ren Dan , Guo Kun yuan , Sun Qing mei , Zhu Bo , Qin Lu ping
Published: July 2022

Scrophularia ningpoensis, a perennial herb native to Southeast China, is used to prepare traditional Chinese medicines for the treatment of various diseases. The root microbiota play pivotal roles in providing hosts with nutrients, promoting growth, suppressing disease, and increasing abiotic stress tolerance. In this study, we investigated the composition of endophytic and rhizospheric fungi in the root microbiomes of S. ningpoensis obtained from different habitats and examined their potential effects on the active constituent contents of the host plant using high-throughput sequencing techniques. Our results indicated that 8709 operational taxonomic units (OTUs) and 5780 defined OTUs shared core rhizosphere OTUs. Ascomycota and Basidiomycota were dominant among the endophytic fungi, whereas Mortierellomycota was distributed in the rhizosphere. The fungal community in the rhizospheric compartment was more diverse than the endophytic community. Redundancy analysis and canonical correspondence analysis of the rhizospheric and endophytic samples revealed that the organic matter, total organic carbon, total nitrogen, and Hg levels were well-correlated with the composition of rhizospheric and endophytic fungal communities. Multiple linear regression analyses facilitated the identification of potentially beneficial fungi whose abundance was correlated with active constituent levels. These fungi could potentially provide valuable information regarding the use of S. ningpoensis in the medicinal plant industry.
Adaptive Regularized Zero-Forcing Beamforming in Massive MIMO with Multi-Antenna Users
Authors: Bobrov Evgeny , Chinyaev Boris , Kuznetsov Viktor , Lu Hao , Minenkov Dmitry , Troshin Sergey , Yudakov Daniil , Zaev Danila
Published: July 2022

Modern wireless cellular networks use massive multiple-input multiple-output (MIMO) technology. This technology involves operations with an antenna array at a base station that simultaneously serves multiple mobile devices which also use multiple antennas on their side. For this, various precoding and detection techniques are used, allowing each user to receive the signal intended for him from the base station. There is an important class of linear precoding called Regularized Zero-Forcing (RZF). In this work, we propose Adaptive RZF (ARZF) with a special kind of regularization matrix with different coefficients for each layer of multi-antenna users. These regularization coefficients are defined by explicit formulas based on SVD decompositions of user channel matrices. We study the optimization problem, which is solved by the proposed algorithm, with the connection to other possible problem statements. We also compare the proposed algorithm with state-of-the-art linear precoding algorithms on simulations with the Quadriga channel model. The proposed approach provides a significant increase in quality with the same computation time as in the reference methods.
Finite Element Method-enhanced Neural Network for Forward and Inverse Problems
Authors: Meethal Rishith Ellath , Kodakkal Anoop , Khalil Mohamed , Ghantasala Aditya , Obst Birgit , Bletzinger Kai-Uwe , Wüchner Roland
Published: July 2022

We introduce a novel hybrid methodology that combines classical finite element methods (FEM) with neural networks to create a well-performing and generalizable surrogate model for forward and inverse problems. The residual from finite element methods and custom loss functions from neural networks are merged to form the algorithm. The Finite Element Method-enhanced Neural Network hybrid model (FEM-NN hybrid) is data-efficient and physics-conforming. The proposed methodology can be used for surrogate models in real-time simulation, uncertainty quantification, and optimization in the case of forward problems. It can be used to update models for inverse problems. The method is demonstrated with examples and the accuracy of the results and performance is compared to the conventional way of network training and the classical finite element method. An application of the forward-solving algorithm is demonstrated for the uncertainty quantification of wind effects on a high-rise buildings. The inverse algorithm is demonstrated in the speed-dependent bearing coefficient identification of fluid bearings. Hybrid methodology of this kind will serve as a paradigm shift in the simulation methods currently used.
Epidemiological study of HPV infection in 40693 women in Putian: a population study based on screening for high-risk HPV infection
Authors: Chen Zhanfei , Lin Hua , Zheng Jinyuan , Chen Zhonghui , Li Jinqiu , Yu Liumin , Cai Lili
Published: July 2022

Background: The infection rate of human papillomavirus (HPV) is high in coastal regions of China. However, the infection of high-risk genotypes of women in Putian City is unknown. Therefore, this study aimed to analyse the epidemiology of high-risk HPV infection among women in Putian and provide a reference for the diagnosis, treatment, and vaccination of cervical cancer in this region.Methods: A total of 40693 female cervical cell exfoliation samples screened for high-risk HPV at the Affiliated Hospital of Putian University from July 2020 to December 2021 were selected. DNA was extracted using a fully automatic extractor. Then, 14 high-risk genotypes of HPV were detected by polymerase chain reaction. The characteristics of HPV infection, distribution of high-risk genotypes, infection types, and TCT classification in different age groups were analysed.Results: Of the 40693 samples, 3899 were infected with HPV, with an infection rate of 9.6%. Accordingly, HPV infection rates gradually increased with age, with statistically significant differences among age groups (χ2=74.03, P<0.01). The infection rates of high-risk HPV52, HPV58, and HPV16 ranked in the top three and increased with age. Single infection was dominant (84.7%), followed by double infections (12.7%). In addition, the cervical cytological classification of 3899 HPV-positive people was as follows: NILM (88.0%), ASC-US (6.6%), ASC-H (1.4%), LSIL (3.2%), and HSIL (0.8%). The study found that the HPV16 infection rate increased with increasing severity of cervical cytology (χ2trend=43.64, P<0.01), while the infection rates of HPV52 (χ2trend=13.89, P<0.01) and HPV58 (χ2trend=13.50, P<0.01) showed opposite trends.Conclusions: The infection rate of female HPV high-risk screening in this region was 9.6%, mainly for single infections. In addition, HPV16, HPV52, and HPV58 were closely related to the severity of cervical cytology. When vaccinated against HPV, the nine-valent vaccine is more effective in preventing infection. Health departments and medical institutions need to strengthen regular HPV screening and science publicity for young and middle-aged women, since both are crucial for preventing cervical cancer.
Determination of MeHg and EtHg in Soils and Sediments with Purge and Trap/Gas Chromatography Cold Atomic Fluorescence Spectrometry
Authors: Zhang Linlin , Wang Qing , Lv Jungang
Published: July 2022

Mercury and its compounds, especially methyl mercury(MeHg) and ethyl mercury(EtHg), are ubiquitous in the environment. It is necessary to the determinate mercury species due to their high toxicity and biomagnification. A novel method is described for the Hg analysis in soil and sediment samples in this study. Samples were extracted with alkaline(KOH/CH3OH), MeHg and EtHg in the extraction solution are derived by sodium tetrapropyl boride to generate volatile methylpropyl mercury and ethylpropyl mercury. After purging and trapping, thermal desorption and gas chromatography separation, Hg compounds were cracked into mercury vapor at high temperature,and finally detected by atomic fluorescence spectrometer, qualitative according to retention time and quantitative with external standard method. Pretreatment and instrumental parameters were optimized in detail. The recoveries of MeHg and EtHg were 83.0%-109% and 90.3%- 96.5%, respectively. The recoveries of the certified standard materials were higher than 90.0%,and the relative standard deviation were less than 1.0%. The method was finally applied to the real samples of black, red and fluvo-aquic soils. The recoveries of MeHg and EtHg were 85.1%-109% and 90.3%-96.3% with the RSDs less than 6.0%. This method was fast and accurate, which could be applied to the analysis of large quantities of samples. After properly adjusting the pretreatment method, this method could also be applied to the analysis of alkyl mercury in environmental organisms (such as fish, shellfish), atmospheric particles and other samples, which hinted its wide application range and promising application prospect.
pH responsive Fe3O4@GT/PVA nanocomposite for drug release of hydroxychloroquine sulfate (HCQ)
Authors: Zandi Ali , Ahangar Hossein Abbastabar , Saffar Ali
Published: July 2022

Recent studies have shown the potential of hydroxychloroquine (HCQ) as a pharmaceutical agent to the treatment of a variety of cancers such as lung, melanoma, breast, pancreatic, and colon cancers. HCQ has been proposed to cure viral diseases such as Covid-19 disease. In this study, Fe3O4@PVA/GT nanocomposite has been synthesized for controlling HCQ release at pH 7.4 and 3.4 in different temperature (37 0C and 40 0C). Taguchi method (L9) with 3 levels and 3 factors was used to optimize the influence of the ratio of PVA (polyvinyl alcohol) to GT (gum tragacanth), Fe3O4 weight percent, and the amount of crosslinking agent (citric acid) on the swelling and releasing rate. The influence of different factors were studied on the swelling and releasing rate such as the ratio of PVA (polyvinyl alcohol) to GT (gum tragacanth), the amount of Fe3O4 and the amount of crosslinking agent (citric acid), by the Taguchi method with 3 factors at 3 levels. The obtained results showed that increasing the amount of Fe3O4 decreased the drug release rate. Increasing the amount of GT to PVA increased the sensitivity of the nanocomposite to pH. So that in neutral pH had the lowest drug release and in acidic pH had the highest drug release. After 18 hours, sample with PVA / GT ratio 0.85:0.15 (wt/wt), CA 3% (wt) and 2% (wt) Fe3O4 had the cumulative drug release 71.57 % and 52.37% in pH 3.4 and 7.4 at 40 0C, respectively.
Simultaneously enhancing the strength and ductility of medium-entropy alloys via manipulating ordered oxygen complexes
Authors: Jiao Meiyuan , Lei Zhifeng , Wu Yuan , Du Jinlong , Li Wenyue , Zhu Xiangyu , Yuan Xiaoyuan , Wang Shudao , Zhu Huihui , Cao Peipei , Liu Xiongjun , Wang Hui , Jiang Suihe , Lu Zhaoping
Published: July 2022

Oxygen solute strengthening is an effective strategy to harden metallic materials, yet, it usually deteriorates the ductility. Ordered oxygen complexes, a state between regular random interstitials and oxides, can simultaneously enhance strength and ductility in high-entropy alloys. However, if such strengthening media can be created and if this particular strengthening mechanism still holds in other alloys remain unclear. Here, we demonstrated that OOCs can be obtained in the single bcc (body-centered-cubic) Ti-Zr-Nb medium-entropy alloys (MEAs) via tailoring the content of bcc-stabilizer Nb and oxygen. It was found that decreasing the bcc phase stability (i.e., lowering the Nb content) enhances the degree of chemical short-range orderings, particularly those enriched in Ti and Zr, and then favors the formation of ordered complexes after doping oxygen. Moreover, the number density of OOCs increases with oxygen contents (\(\le 3.0\)at.%) in a given alloy, but addition of excessive oxygen (\(>3.0\)at.%) leads to grain boundary segregation. As a result, the tensile yield strength is appreciably enhanced by ~ 75% and ductility is substantially improved by ~ 164% when doping the Ti-30Zr-14Nb MEA with 3.0 at.% oxygen. Our work not only manifests the universality of the novel interstitial strengthening mechanism, but also offers insights into developing advanced metallic materials with localized atomic orderings.
Valeric Acid acts as a novel HDAC3 inhibitor against Prostate Cancer
Authors: Han Rui , Yang Hongxing , Li Ya , Ling Changquan , Lu Lingeng
Published: July 2022

Prostate cance is the second cause of cancer-related deaths in men worldwide, and new agents for curing the disease are still needed. In this study, we theorectically and experimentally demonstrated that valeric acid (VA) was a HDAC inhibitor, anticancer efficacy of VA in prostate cancer cells was also observed using either 2D or 3D culture systems. VA was cytotoxic for prostate cancer cells but low-toxic to normal cells. VA significantly inhibited E2F1/E2F3 expression but increased CASP3 activity. In vivo mouse models further showed its anticancer activity and potential property of chemosensitizer with promoting apoptosis. The findings suggest that VA acts as a HDAC3 inhibitor with anti-cancer effect on prostate cancer by regulating E2F1/E2F3/CASP3 axis.
Emotional dysregulation in endometriosis women with cyclical and non-cyclical chronic pelvic pain
Authors: Rodríguez-Lozano Dulce Carolina , Meza-Rodríguez María del Pilar , Cruz-Orozco Olivier Paul , Sánchez-Ramírez Brenda , Olguin-Ortega Andrea , Silvestri-Tomassoni José Roberto , Corona-Barsse Guillermo , Escobar-Ponce Luis Fernando , Solis-Paredes Juan Mario , Dominguez-Trejo Benjamín , Camacho-Arroyo Ignacio
Published: July 2022

Background: Endometriosis is a pathophysiological condition characterized by glands and stroma outside the uterus in regions such as the bladder, ureter, fallopian tubes, peritoneum, ovaries, and even in extra pelvic sites. One of the main clinical problems of endometriosis is chronic pelvic pain (CPP), which considerably affects the patients’ quality of life. Patients with endometriosis may, cyclically or non-cyclically (80% of cases) experience CPP. High levels of anxiety and depression have been described in patients with endometriosis related to CPP; however, this has not been evaluated in endometriosis women with different types of CPP. Methods: This work aimed to determine if there are differences in emotional dysregulation in patients with cyclical and non-cyclical CPP. 49 women diagnosed with endometriosis presenting cyclical and non-cyclical CPP answered several batteries made up of Mini-Mental State Examination, Visual Analog Scale, Beck´s Depression Inventory, State Trait-Anxiety Inventory, and Generalized Anxiety Inventory. Results: We observed that patients with non-cyclical CPP exhibited higher levels of depression and anxiety (trait-state and generalized anxiety) than patients with cyclical pain. No differences were observed in pain intensity, but there was a higher probability of developing emotional dysregulation (anxiety or depression) in patients with non-cyclical CPP. No differences were observed in cognitive impairment. Conclusions:Our data suggest that patients with non-cyclical CPP present a higher emotional dysregulation than those with cyclical pain.
Machine learning for prediction of bronchopulmonary dysplasia-free survival among very preterm infants
Authors: Leigh Rebekah M. , Pham Andrew , Rao Srinandini S. , Vora Farha M. , Hou Gina , Kent Chelsea , Rodriguez Abigail , Narang Arvind , Tan John B. C. , Chou Fu-Sheng
Published: July 2022

Background
Bronchopulmonary dysplasia (BPD) is one of the most common and serious sequelae of prematurity. Prompt diagnosis using prediction tools is crucial for early intervention and prevention of further adverse effects. This study aims to develop a machine learning-based BPD-free survival prediction tool based on the concept of the developmental origin of BPD.
Methods
Datasets comprising perinatal factors and early postnatal respiratory support were used for initial model development, followed by combining the two models into a final ensemble model using logistic regression. Simulation of clinical scenarios was performed.
Results
Data from 689 infants were included in the study. We randomly selected 80% of which was used for model development and used the remainder 20% for validation. The performance of the final model was assessed by receiver operating characteristics which showed 0.921 (95% CI: 0.899–0.943) and 0.899 (95% CI: 0.848–0.949) for the training and the validation datasets, respectively. Simulation data suggests that extubating to CPAP is superior to NIPPV in BPD-free survival. Additionally, successful extubation may be defined as no reintubation for 9 days following initial extubation.
Conclusions
Machine learning-based BPD prediction based on perinatal features and respiratory data may have clinical applicability to promote early targeted intervention in high-risk infants.
β-Asarone Promotes Autophagy by Inhibiting the PI3K/Akt/mTOR Pathway in a Rat Model of Depression in Parkinson’s Disease
Authors: Wang Zhifang , Wang Nanbu , Zhang Qinxin , Kang Jian , Fang Yongqi , Ning Baile , Li Ling
Published: July 2022

Depression in Parkinson’s disease (DPD) is a major non-motor symptom of Parkinson’s disease (PD), and is accompanied by monoamine neurotransmitter degradation in DPD rats. Autophagy is neuroprotective because it helps degrade abnormal deposition of α-synuclein (α-syn). The PI3K/Akt/mTOR pathway is a key autophagy pathway. β-Asarone, derived from Acorus tatarinowii Schott, has anti-PD and anti-depression effects. This study aimed to investigate the mechanism of action of β-asarone in the treatment of DPD model rats. We found that oral administration of β-asarone (7.5, 15, and 30 mg/kg) for 4 weeks increased dopamine, L-DOPA (levodopa; L-3,4-dihydroxyphenylalanine), and serotonin levels in the midbrain and decreased α-syn levels in DPD model rats compared with model group. As the neurotransmitter levels changed, the expression of Beclin-1 increased while that of p62 decreased in β-asarone-treated DPD rats compared with that in controls. Transmission electron microscopy (TEM) showed that β-asarone treatment also improved the number of autophagosomes and decreased mitochondrial damage in the striatum compared with that in untreated animals. These results suggest that β-asarone activates autophagy. Furthermore, western blotting and transmission electron microscopy analyses revealed that the PI3K/Akt/mTOR pathway played an important role in β-asarone treatment-induced activation of autophagy. Furthermore, β-asarone increased the levels of Beclin-1 protein and reduced the expression of p62, p-PI3K, p-AKT, and p-mTOR in the hippocampus. Lastly, transmission electron microscopy results demonstrated that the β-asarone-treated animals displayed abundant autophagosomes in the prefrontal cortex. These findings suggest that β-asarone exerts neuroprotective effects in this DPD model via the promotion of autophagy, and may be an effective therapeutic agent for DPD treatment.
Developmental miR-17-92 cluster and sfmbt2 miRNA cluster can’t rescue the abnormal embryonic development caused by the obstructive epididymal environment
Authors: Wu Xunwei , He Xiaomei , Liu Qian , Li Honggang
Published: July 2022

Background
Sperms during epididymal transit acquire microRNAs(miRNAs), which are crucial for embryonic development. However, whether miRNAs transmitted from an obstructive epididymal environment affect embryonic development remains unknown. The purpose of the present study was to investigate the effects of an obstructive epididymal environment on embryonic development.
Method
The control group (CON) and the obstructive epididymal environment group(OEE) were treated with sham operation and vasectomy, respectively. The effects of an obstructive epididymal environment on testis, epididymis, sperm, and embryonic development were analyzed via hematoxylin and eosin staining (HE staining), sperm quality test, epididymosomes fusion, intracytoplasmic sperm injection (ICSI) and embryo transfer. Moreover, sRNA sequence, RT-qPCR, and DNA methylation were conducted to investigate the regulation of sperm miRNAs by an obstructive epididymal environment. miRNAs microinjection and embryo transfer were used to explore the impacts of sperm miRNAs on embryonic development.
Results
The long obstructive interval impaired the morphology of the testis and epididymis. In comparison to the CON group, the OEE group had lower sperm quality and developmental potential. Sperm miRNAs profiles were also altered in the OEE group, particularly the developmental miR-17-92 cluster and Sfmbt2 miRNA clusters; the expression of these two miRNA clusters was regulated via epididymal DNA methylation; however, the disruption of neither the miR-17-92 cluster nor the Sfmbt2 miRNA clusters into normal zygotes did not impair embryonic development.
Conclusion
The obstructive epididymal environment influences embryonic development and the abundance of the sperm miR-17-92 cluster and the sfmbt2 miRNA cluster, but these miRNA clusters are not the cause of abnormal embryo development. It implies that epididymis is important in early embryonic development and may play a potential role in sperm epigenome.
Plumbagin rescues the granulosa cell's pyroptosis by reducing WTAP-mediated N6-methylation in polycystic ovary syndrome
Authors: Cai Zhaowei , He Shaojuan , Liu Rongju , Zhou Liling , Zhao Li
Published: July 2022

The survival of ovary granulosa cells (GC) is critical in the initiation and progression of polycystic ovary syndrome (PCOS) in females. Here, we found that the PCOS process is accompanied by massive GC pyroptosis resulting from Caspase-1 inflammasome activation. Administration of plumbagin, an effective compound isolated from plant medicine, can prevent the pyroptosis of GC and the onset of PCOS. Mechanistic study indicates the over activation of inflammasome in GC is due to the upregulation of WTAP, a key regulator of the RNA N6-methylase complex. WTAP mediates the mRNA N6-methylation of NLRP3 inflammasome component ASC and enhances ASC RNA stability, which results in the overactivation of the inflammasome in GCs from the PCOS model. Plumbagin treatment suppresses the WTAP-mediated N6-methylation of ASC mRNA and reduces the pyroptosis of GCs. This study supports the profound potential of plumbagin in PCOS treatment.