bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic epidemiology of late blight in Australia using ancient DNA
Authors: Caruana Brittney M. , de Boer Rudolf F , Rodoni Brendan , Cogan Noel O.I. , Edwards Jacqueline
Published: August 2023

Late blight caused by Phytophthora infestans is the most destructive disease of potatoes worldwide and is most notable as the cause of the Irish potato famine of the mid 1840’s. Whole mitochondrial genome sequences were generated from 44 Australian, two UK and one US isolates of P. infestans stored as either herbarium specimens or as recent samples on FTA cards, spanning the period 1873–2019. Mitochondrial (mt) genome sequence comparison confirmed that over the past c. 110 years, an old A1 strain of P. infestans has been present in Australia. There was evidence, however, that P. infestans had been introduced on multiple separate occasions in the early 1900s prior to the introduction of quarantine measures. The 44 Australian samples included six infected specimens of the Australian native kangaroo apple (Solanum aviculare) collected in 1911. The P. infestans mt genome sequences from these isolates clustered with P. infestans from infected potatoes collected in Victoria (1910, 1911, 1986, 1989), South Australia (1909, 2003) and Tasmania (1910, 2019), supporting the hypothesis that Australia has one old strain that has survived since the first arrival of the pathogen into the country. The study highlights the success of Australia’s potato biosecurity setting and supports the need for ongoing surveillance and biosecurity measures to prevent the introduction of the newer more aggressive strains of the pathogen.
How do work challenges interact with health management in dairy farms?
Authors: Gotti Vinciane , Manoli Claire , Dedieu Benoît
Published: August 2023

Herd health management is a critical issue for the future of dairy systems. The right combination of preventive and curative practices will depend on management system, level of work productivity, and self-sufficiency objectives, and will entail specific skills and work organizations. However, the combination of work dimensions and animal health management has rarely been explored in the literature on a livestock farming system scale. The Grand Ouest region of France spans a diverse array of livestock farming systems that can serve to design herd health management indicators, farming objectives and work arrangements, and explore their linkages. Here we ran semi-structured interviews on 10 dairy farms, analyzed the farmers’ discourses, and built 7 variables and 25 modalities that, for the first time, cover three components, namely herd health, farming objectives and work arrangements, and we tested various associations between these variables. Our interview data confirms that consultants and veterinarians have a key role to play in building a pool of skills adapted to various types of health management system. Data suggests linkages between prevention measures, alternative or conventional curative interventions, and work-related parameters.
Low-Thrust Lunar Capture Leveraging Nonlinear Orbit Control
Authors: Pontani Mauro , Pustorino Marco
Published: August 2023

Nonlinear orbit control with the use of low-thrust propulsion is proposed as an effective strategy for autonomous guidance of a space vehicle directed toward the Moon. Orbital motion is described in an ephemeris model, with the inclusion of the most relevant perturbations. Unfavorable initial conditions, associated with weak, temporary lunar capture, are considered, as representative conditions that may be encountered in real mission scenarios. These may occur when the spacecraft is released in nonnominal flight conditions, which would naturally lead it to impact the Moon or escape the lunar gravitational attraction. To avoid this, low-thrust propulsion, in conjunction with nonlinear orbit control, is employed, to drive the space vehicle toward two different, prescribed, low-altitude lunar orbits. Nonlinear orbit control leads to identifying a saturated feedback law (for the low-thrust magnitude and direction) that is proven to enjoy global stability properties. The guidance strategy at hand is successfully tested on three different mission scenarios. Then, the capture region is identified, and includes a large set of initial conditions for which nonlinear orbit control with low-thrust propulsion is effective to achieve lunar capture and final orbit acquisition. For the purpose of achieving lunar capture, low-thrust propulsion is shown to be more effective if ignited at aposelenium.
Influence of railway wheel tread damage on wheel–rail impact loads and the durability of wheelsets
Authors: Maglio Michele , Vernersson Tore , Nielsen Jens C. O. , Ekberg Anders , Kabo Elena
Published: August 2023

Dynamic wheel–rail contact forces induced by a severe form of wheel tread damage have been measured by a wheel impact load detector during full-scale field tests at different vehicle speeds. Based on laser scanning, the measured three-dimensional damage geometry is employed in simulations of dynamic vehicle–track interaction to calibrate and verify a simulation model. The relation between the magnitude of the impact load and various operational parameters, such as vehicle speed, lateral position of wheel–rail contact, track stiffness and position of impact within a sleeper bay, is investigated. The calibrated model is later employed in simulations featuring other forms of tread damage; their effects on impact load and subsequent fatigue impact on bearings, wheel webs and subsurface initiated rolling contact fatigue of the wheel tread are assessed. The results quantify the effects of wheel tread defects and are valuable in a shift towards condition-based maintenance of running gear, and for general assessment of the severity of different types of railway wheel tread damage.
Molecular study of the presence and transcriptional activity of HPV in semen
Authors: Faja F. , Pallotti F. , Bianchini S. , Buonacquisto A. , Cicolani G. , Conflitti A. C. , Fracella M. , Cavallari E. N. , Sciarra F. , Pierangeli A. , Paoli D. , Lenzi A. , Antonelli G. , Lombardo F. , Gianfrilli D.
Published: August 2023

Purpose

Human Papillomavirus (HPV) in semen represents a controversial topic. Recent evidence suggests a correlation with poor semen quality, but its detection is still unstandardized in this biological fluid. Thus, the aims of this study were to verify the ability of nested PCR to reveal HPV-DNA in semen; to evaluate association of seminal HPV with sperm parameters and risk factors for infection; to investigate the rate of HPV-DNA positivity in patients with and without risk factors; to assess HPV transcriptional activity.

Methods

We enrolled sexually active men and collected clinical and anamnestic data during andrological and sexually transmitted infections (STIs) evaluation. For each patient, we performed semen analysis and nested PCR to detect HPV-DNA in semen. In positive semen samples, we proceeded with genotyping and RNA quantification to detect HPV transcriptional activity.

Results

We enrolled 185 men (36.0 ± 8.3 years), of which 85 with (Group A) and 100 without HPV risk factors (Group B). Nested PCR was able to reveal HPV-DNA in semen, discovering a prevalence of 8.6% (11.8% in Group A and 6% in Group B, respectively). We observed no correlation between sperm quality and seminal HPV. Genital warts and previous anogenital infection were significantly associated with the risk of HPV positivity in semen. Moreover, no viral transcriptional activity was detected in positive semen samples.

Conclusions

Our study suggests that searching for seminal HPV could be important in patients both with and without risk factors, especially in assisted reproduction where the risk of injecting sperm carrying HPV-DNA is possible.
A new enigmatic lacustrine trackway in the upper Miocene of the Sierra de las Cabras (Jumilla, Murcia, Spain)
Authors: Mayoral Eduardo , Herrero Cayetano , Herrero Emilio , Martín‑Chivelet Javier , Pérez‑Lorente Félix
Published: August 2023

A new fossil trackway is described in the upper lacustrine Miocene in the Prebetic Zone of the Iberian Peninsula, in Jumilla town (Murcia region) called Aenigmatipodus jumillensis nov. ichnogen. nov. ichnosp. This trackway consists of a pattern made up of sets of three tracks or triads, which are subparallel to each other, arranged in alternate groups. Each track presents a depression formed by a central body that is three times as long as it is wide, with straight or slightly curved walls, with two shorter bodies placed at the ends, one of the ends being shorter and more pronounced than the opposite, which is longer and stretched. All the biomechanical possibilities compatible with an anatomical design that could leave the impression of three alternate triads of tracks are analysed. The supports are only from the extremities on one side of the organism (left or right), the displacement being by translation. It is concluded that it had to be a large arthropod (metre scale), with a hexapod or decapod (less probably octopod), which had to be dragged laterally by a current in a very shallow lake or wetland environment. To date, no fossil organism is known, nor its current equivalent, that corresponds to these characteristics.
Prospection as a Sustainability Virtue: Imagining Futures for Intergenerational Ethics
Authors: Cadilha Susana , Guedes Vaz Sofia
Published: August 2023

This paper explores the relevance of intergenerational ethics in the context of climate change and argues that virtue ethics provides a suitable framework for addressing these ethical concerns. We suggest the inclusion of a new virtue, calledprospection, which involves cultivating the ability to think and care deeply about the future, navigate its inherent uncertainties constructively, and ensure the availability of sustainable options for future generations. We suggest that thinking and imagining sustainable futures trigger good dispositions towards future humans, non-humans and nature and that ought to be part of the definition of a good life. We posit that fostering this disposition can inspire and motivate present actions that effectively mitigate climate change. Virtue ethics implies an element of timelessness in morality because the future tends to be there by default; withprospection, the future will be there by design, reinforcing its importance in intergenerational ethics.
Metabolomics-directed nanotechnology in viral diseases management: COVID-19 a case study
Authors: El-Derany Marwa O. , Hanna Diana M. F. , Youshia John , Elmowafy Enas , Farag Mohamed A. , Azab Samar S.
Published: August 2023

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently regarded as the twenty-first century’s plague accounting for coronavirus disease 2019 (COVID-19). Besides its reported symptoms affecting the respiratory tract, it was found to alter several metabolic pathways inside the body. Nanoparticles proved to combat viral infections including COVID-19 to demonstrate great success in developing vaccines based on mRNA technology. However, various types of nanoparticles can affect the host metabolome. Considering the increasing proportion of nano-based vaccines, this review compiles and analyses how COVID-19 and nanoparticles affect lipids, amino acids, and carbohydrates metabolism. A search was conducted on PubMed, ScienceDirect, Web of Science for available information on the interrelationship between metabolomics and immunity in the context of SARS-CoV-2 infection and the effect of nanoparticles on metabolite levels. It was clear that SARS-CoV-2 disrupted several pathways to ensure a sufficient supply of its building blocks to facilitate its replication. Such information can help in developing treatment strategies against viral infections and COVID-19 based on interventions that overcome these metabolic changes. Furthermore, it showed that even drug-free nanoparticles can exert an influence on biological systems as evidenced by metabolomics.

Graphical abstract
The Sex Economy
Authors: O'Connor Monica
Published: August 2023

The discourse surrounding prostitution is increasingly one of sexual commerce, transaction and commercial exchange. The 'sex economy'and the consumer demand for it is often discussed both as a legitimate economic business, in which women have control, and as employment comparable to other forms of low-paid work. So much so, that in some countries it is being seen as a service that should be regulated and given a labour-rights framework.

Drawing on extensive and detailed research, Monica O'Connor challenges the suggestion that the sale of women's bodies as commodities can ever be acceptable, and that the male consumer has an acceptable right to buy sexual acts from another person. She disproves the claim that 'sex work' is a lucrative occupation for impoverished women and girls that can be considered for regulation as part of the normal economy. She lays bare the harm that 'normalising' the sex trade does on women's lives, gender equality and on society as a whole, and exposes the realities that constrain and control women locked in prostitution, debunking the notions of choice and agency.
Capitalism, Socialism and Property Rights
Authors: Machaj Mateusz
Published: August 2023

The comparative analysis of socialist and capitalist economic systems has given rise to a voluminous literature in the history of economic thought, yet detailed analysis of the 'market socialism' model, which seeks to imitate the functional efficiency of capitalism by simulating a competitive economy, has been relatively neglected. In this work, Mateusz Machaj seeks to redress this imbalance by providing an in-depth examination of one of the defining issues that separates capitalism from socialism - the system of ownership, or property rights - which, when explored, highlight fundamental problems in the market socialism model.

Taking a broadly Austrian perspective, he shows that the mechanism of efficiency in market socialism is unable to play the part ascribed to it by its theoreticians, because it disregards the fact that property rights are fundamental to the shaping of prices and thus the abolition of ownership in market socialism makes its mechanism of efficiency a fiction. Indeed, the author argues, the economic terms used in the model of market capitalism only mirror the names of the real economic variables that cause capitalism to be efficient, not their functions.

The book offers new and original insights into the theory of competition, theories of pricing, property laws, the relation between law and economics, as well as the economics of the market socialism model. It will be of interest to a wide range of heterodox economists.
The Federal Reserve and its Founders
Authors: Naclerio Richard A.
Published: August 2023

To fully understand the Federal Reserve and its role today we need to examine its origins and the men who founded it. Using extensive archival sources, Richard Naclerio investigates the highly secretive events that surrounded the Fed's creation and the bankers, financiers and tycoons that shaped both its organization and the role it was to play over the next century. The motivations of this handful of men who created the first draft of the Federal Reserve Act are explored, and the business ties and shared ideologies that bound them together revealed. A story of vested interest and the pursuit of power, the book sheds new light on the creation of one of the world's most important financial institutions.
Analysing Corruption
Authors: Hough Dan
Published: August 2023

Repeated corruption scandals and the efforts of the international political community to find ways to counteract them have compelled economists, anthropologists and political scientists to confront corruption as a subject for serious academic research. This textbook introduces students to the field of corruption analysis and the challenges facing its researchers.

The book explores the definitional challenges, the problems of measurement and the methodologies that underpin the standard corruption indices. The key drivers of corrupt practice are identified and the arguments used to understand the causes of corruption are outlined. The book looks at what works in the fight against corruption, including international conventions and organizations, and policy initiatives at the national level. The role of third sector organizations, the so-called 'anti-corruption industry' and the work of citizen activists and 'armchair auditors' are also explored.

Analysing Corruption provides an authoritative and engaging introduction to a subject that is the largest public policy challenge that the state faces in many parts of the world. It is suitable for courses in politics, public policy, public administration, development studies and anthropology. It will also be of value to those working in NGOs and charities helping to shape anti-corruption thinking.
Dynamics of fixed-volume pinned films – dealing with a non-self-adjoint thin-film problem
Authors: Gabay Israel , Bacheva Vesna , Ilssar Dotan , Bercovici Moran , Ramos Antonio , Gat Amir
Published: August 2023

The use of thin liquid films has expanded beyond lubrication and coatings, and into applications in actuators and adaptive optical elements. In contrast to their predecessors, whose dynamics can be typically captured by modelling infinite or periodic films, these applications are characterized by a finite amount of liquid in an impermeable domain. The global mass conservation constraint, together with common boundary conditions (e.g. pinning), create quantitatively and qualitatively different dynamics than those of infinite films. Mathematically, this manifests itself as a non-self-adjoint problem. This work presents a combined theoretical and experimental study for this problem. We provide a time-dependent closed-form analytical solution for the linearized non-self-adjoint system that arises from these boundary conditions. We highlight that, in contrast to self-adjoint problems, here, special care should be given to deriving the adjoint problem to reconstruct the solution based on the eigenfunctions properly. We compare these solutions with those obtained for permeable and periodic boundary conditions, representing common models for self-adjoint thin-film problems. We show that, while the initial dynamics is nearly identical, the boundary conditions eventually affect the film deformation as well as its response time. To experimentally illustrate the dynamics and to validate the theoretical model, we fabricated an experimental set-up that subjects a thin liquid film to a prescribed normal force distribution through dielectrophoresis, and used high-frame-rate digital holography to measure the film deformation in real time. The experiments agree well with the model and confirm that confined films exhibit a different behaviour which could not be predicted by existing models.
Collective Narcissism and Hungary’s Kin-State Policy after 2010
Authors: Hettyey András
Published: August 2023

This article analyzes Hungary’s kin-state policy starting from the premise that the concept of collective narcissism most succinctly captures its emotional foundations. I look to substantiate this claim by examining at a wide range of sources on how Hungary feels toward neighboring states and show the preponderance of emotions connected to collective narcissism. The real-life consequences of collective narcissism are demonstrated through a case study of the relationship between Hungary and Ukraine before and after the Russian attack of 2022. Overall, I find that anger is the master emotion of Hungary’s kin-state policy, resulting in a lack of solidarity and characteristic self-centeredness in Hungary’s foreign policy, which cannot be explained by rational factors alone.
The origin of planetary winds
Authors: Kubyshkina Daria
Published: August 2023

Atmospheric escape is a fundamental phenomenon shaping the structure and evolution of planetary atmospheres. Physics of planetary winds range from global processes such as tidal interactions with the host star, through large-scale hydrodynamic outflow, to essentially microphysical kinetic effects, including Jeans-like escape and the interaction of planetary atmospheres with stellar winds and the own magnetic fields of planets. Each of these processes is expected to be most relevant for planets of different properties and at different stages in planetary and stellar evolution. Thus, it is expected that the hydrodynamic outflow guides the evolution of hydrogen-dominated atmospheres of planets having low masses (below that of Neptune) and/or close-in orbits, while the kinetic effects are most important for the long-term evolution of planets with secondary atmospheres, similar to the inner planets in the Solar System. Finally, each of these processes is affected by the interaction with stellar winds.
Global Earthquake Analysis Seeks Elusive Foreshock Signal
Authors: Scharping Nathaniel
Published: August 2023

Spotting foreshocks for what they are could help seismologists forecast large earthquakes.
Europa's Perturbed Fields and Induced Dipole Affect Energetic Proton Depletions During Distant Alfvén Wing Flybys
Authors: Huybrighs H. L. F. , Blöcker A. , Roussos E. , van Buchem C. , Futaana Y. , Holmberg M. K. G. , Goetz C. , Witasse O.
Published: August 2023

We investigate the causes of energetic proton (80–540 keV) depletions measured during the two most distant flybys of Europa by Galileo, E17 and E25A, which encountered the Alfvén wings. First, by simulating the proton flux with a Monte Carlo particle tracing code we investigate the effect of: electromagnetic field perturbations, the induced dipole, atmospheric charge exchange and plumes. Inhomogeneous fields associated with the Alfvén wings and the ionosphere strongly affect the depletions. For homogeneous fields the depletion along the trajectory is focused on a narrow pitch angle range and has no structure, whereas the depletion for perturbed (inhomogeneous) fields represents a wider and complex structure. Furthermore, also the induced dipole alters the depletion structure. The effect of plumes (density 2.5 × 1015 m−3) and charge exchange on the proton depletion is minor. Second, we compare the simulations to the proton measurements. The simulations with inhomogeneous fields describe the data qualitatively better than the homogeneous case, suggesting that indeed field perturbations are responsible for the measured losses. We attribute discrepancies between the simulations and the proton measurements to discrepancies between the simulated and real fields. We argue that simulating the fields along the trajectory is a good first step, but that ideally the energetic ion flux is reconstructed well to gain confidence in the interpretation of the simulated magnetic field. In conclusion, energetic ion observations along distant flybys through the Alfvén wings are suitable for isolating the characteristics of the global configuration of the magnetospheric interaction region of Europa (or other moons).
Toward Improved Real‐Time Rainfall Intensity Estimation Using Video Surveillance Cameras
Authors: Zheng Feifei , Yin Hang , Ma Yiyi , Duan Huan‐Feng , Gupta Hoshin , Savic Dragan , Kapelan Zoran
Published: August 2023

Under global climate change, urban flooding occurs frequently, leading to huge economic losses and human casualties. Extreme rainfall is one of the direct and key causes of urban flooding, and accurate rainfall estimates at high spatiotemporal resolution are of great significance for real‐time urban flood forecasting. Using existing rainfall intensity measurement technologies, including ground rainfall gauges, ground‐based radar, and satellite remote sensing, it is challenging to obtain estimates of the desired quality and resolution. However, an approach based on processing distributed surveillance camera network imagery through machine learning algorithms to estimate rainfall intensities shows considerable promise. Here, we present a novel approach that first extracts raindrop information from the surveillance camera images (rather than using the raw imagery directly), followed by the use of convolutional neural networks to estimate rainfall intensity from the resulting raindrop information. Evaluation of the approach on 12 rainfall events under both daytime and nighttime conditions shows that generalization ability, and especially nighttime predictive performance, is significantly improved. This represents an important step toward achieving real‐time, high spatiotemporal resolution, measurement of urban rainfall at relatively low cost.
Metformin accelerates bone fracture healing by promoting type H vessel formation through inhibition of YAP1/TAZ expression
Authors: Ruan Zhe , Yin Hao , Wan Teng-Fei , Lin Zhi-Rou , Zhao Shu-Shan , Long Hai-Tao , Long Cheng , Li Zhao-Hui , Liu Yu-Qi , Luo Hao , Cheng Liang , Chen Can , Zeng Min , Lin Zhang-Yuan , Zhao Rui-Bo , Chen Chun-Yuan , Wang Zhen-Xing , Liu Zheng-Zhao , Cao Jia , Wang Yi-Yi , Jin Ling , Liu Yi-Wei , Zhu Guo-Qiang , Zou Jing-Tao , Gong Jiang-Shan , Luo Yi , Hu Yin , Zhu Yong , Xie Hui
Published: August 2023

Due to increasing morbidity worldwide, fractures are becoming an emerging public health concern. This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures. Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis. Here, we show that metformin accelerated fracture healing in both osteoporotic and normal mice. Moreover, metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing. Mechanistically, metformin increased the expression of HIF-1α, an important positive regulator of type H vessel formation, by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells (HMECs). The results of HIF-1α or YAP1/TAZ interference in hypoxia-cultured HMECs using siRNA further suggested that the enhancement of HIF-1α and its target genes by metformin is primarily through YAP1/TAZ inhibition. Finally, overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair. In summary, our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.
ZFP36 loss-mediated BARX1 stabilization promotes malignant phenotypes by transactivating master oncogenes in NSCLC
Authors: Zhang Tongjia , Qiu Lizhen , Cao Jiashun , Li Qiu , Zhang Lifan , An Guoshun , Ni Juhua , Jia Hongti , Li Shuyan , Li Kailong
Published: August 2023

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer, with high morbidity and mortality worldwide. Although the dysregulation of BARX1 expression has been shown to be associated with malignant cancers, including NSCLC, the underlying mechanism remains elusive. In this study, we identified BARX1 as a common differentially expressed gene in lung squamous cell carcinoma and adenocarcinoma. Importantly, we uncovered a novel mechanism behind the regulation of BARX1, in which ZFP36 interacted with 3’UTR of BARX1 mRNA to mediate its destabilization. Loss of ZFP36 led to the upregulation of BARX1, which further promoted the proliferation, migration and invasion of NSCLC cells. In addition, the knockdown of BARX1 inhibited tumorigenicity in mouse xenograft. We demonstrated that BARX1 promoted the malignant phenotypes by transactivating a set of master oncogenes involved in the cell cycle, DNA synthesis and metastasis. Overall, our study provides insights into the mechanism of BARX1 actions in NSCLC and aids a better understanding of NSCLC pathogenesis.
Visualization of π-hole in molecules by means of Kelvin probe force microscopy
Authors: Mallada B. , Ondráček M. , Lamanec M. , Gallardo A. , Jiménez-Martín A. , de la Torre B. , Hobza P. , Jelínek P.
Published: August 2023

Submolecular charge distribution significantly affects the physical-chemical properties of molecules and their mutual interaction. One example is the presence of a π-electron-deficient cavity in halogen-substituted polyaromatic hydrocarbon compounds, the so-called π-holes, the existence of which was predicted theoretically, but the direct experimental observation is still missing. Here we present the resolution of the π-hole on a single molecule using the Kelvin probe force microscopy, which supports the theoretical prediction of its existence. In addition, experimental measurements supported by theoretical calculations show the importance of π-holes in the process of adsorption of molecules on solid-state surfaces. This study expands our understanding of the π-hole systems and, at the same time, opens up possibilities for studying the influence of submolecular charge distribution on the chemical properties of molecules and their mutual interaction.
Circumventing the phonon bottleneck by multiphonon-mediated hot exciton cooling at the nanoscale
Authors: Jasrasaria Dipti , Rabani Eran
Published: August 2023

Nonradiative processes govern efficiencies of semiconductor nanocrystal (NC)-based devices. A central process is hot exciton cooling, or the nonradiative relaxation of a highly excited electron/hole pair to form a band-edge exciton. Due to quantum confinement effects, the timescale and mechanism of cooling are not well understood. A mismatch between electronic energy gaps and phonon frequencies has led to the hypothesis of a phonon bottleneck and extremely slow cooling, while enhanced electron-hole interactions have suggested ultrafast cooling. Experimental measurements of the cooling timescale range six orders of magnitude. Here, we develop an atomistic approach to describe phonon-mediated exciton dynamics and simulate cooling in NCs of experimentally relevant sizes. We find that cooling occurs on ~30 fs timescales in CdSe NCs, in agreement with the most recent measurements, and that the phonon bottleneck is circumvented through a cascade of multiphonon-mediated relaxation events. Furthermore, we identify NC handles for tuning the cooling timescale.
Effects of positive and negative social feedback on motivation, evaluative learning, and socio-emotional processing
Authors: Sobczak Alexandra , Bunzeck Nico
Published: August 2023

Social rewards and punishments are strong motivators. Since experimental work has focused on young adults using simplistic feedback, the effects of more naturalistic stimuli on motivation, evaluative learning, and socio-emotional processing with advanced age remain unclear. Therefore, we compared the effects of static (photos) vs dynamic (videos) social feedback in a social incentive delay (SID) task in young (18–35 years) and older adults (50–84 years) with neutral, positive, and negative feedback, on response times (RTs), and assessed the emotional valence of feedback cues and feedback videos. We found that anticipating positive and negative social feedback accelerated RTs regardless of age and without additional effects of video feedback. Furthermore, the results suggest a valence transfer from positive feedback videos to predictive cues in both groups (i.e., evaluative learning). Finally, older adults reported less pronounced negative affect for negative feedback videos, indicating age differences in socio-emotional processing. As such, our findings foster our understanding of the underlying cognitive and emotional aspects involved in the processing of social rewards and punishments.
The relevance of a right scale for sampling when studying high-resolution behavioral dynamics
Authors: Barberis L. , Simian C. , Marin R. H. , Kembro J. M.
Published: August 2023

Many species used in behavioral studies are small vertebrates with high metabolic rates and potentially enhanced temporal resolution of perception. Nevertheless, the selection of an appropriate scales to evaluate behavioral dynamics has received little attention. Herein, we studied the temporal organization of behaviors at fine-grain (i.e. sampling interval ≤1s) to gain insight into dynamics and to rethink how behavioral events are defined. We statistically explored high-resolution Japanese quail (Coturnix japonica) datasets encompassing 17 defined behaviors. We show that for the majority of these behaviors, events last predominately <300ms and can be shorter than 70ms. Insufficient sampling resolution, even in the order of 1s, of behaviors that involve spatial displacement (e.g. walking) yields distorted probability distributions of event durations and overestimation of event durations. Contrarily, behaviors without spatial displacement (e.g. vigilance) maintain non-Gaussian, power-law-type distributions indicative of long-term memory, independently of the sampling resolution evaluated. Since data probability distributions reflect underlying biological processes, our results highlight the importance of quantification of behavioral dynamics based on the temporal scale pertinent to the species, and data distribution. We propose a hierarchical model that links diverse types of behavioral definitions and distributions, and paves the way towards a statistical framework for defining behaviors.
Bird’s Eye View feature selection for high-dimensional data
Authors: Brahim Belhaouari Samir , Shakeel Mohammed Bilal , Erbad Aiman , Oflaz Zarina , Kassoul Khelil
Published: August 2023

In machine learning, an informative dataset is crucial for accurate predictions. However, high dimensional data often contains irrelevant features, outliers, and noise, which can negatively impact model performance and consume computational resources. To tackle this challenge, the Bird’s Eye View (BEV) feature selection technique is introduced. This approach is inspired by the natural world, where a bird searches for important features in a sparse dataset, similar to how a bird search for sustenance in a sprawling jungle. BEV incorporates elements of Evolutionary Algorithms with a Genetic Algorithm to maintain a population of top-performing agents, Dynamic Markov Chain to steer the movement of agents in the search space, and Reinforcement Learning to reward and penalize agents based on their progress. The proposed strategy in this paper leads to improved classification performance and a reduced number of features compared to conventional methods, as demonstrated by outperforming state-of-the-art feature selection techniques across multiple benchmark datasets.
Ecological, morpho-agronomical, and nutritional characteristics of Sulla flexuosa (L.) Medik. ecotypes
Authors: Boukrouh S. , Noutfia A. , Moula N. , Avril C. , Louvieaux J. , Hornick J. L. , Chentouf M. , Cabaraux J. F.
Published: August 2023

The present work was part of assessing wild genetic plant resources of forage interest in Northern Morocco and aimed to study the agro-morphology and nutritional value of Sulla flexuosa (L.) Medik. (Hedysarum flexuosum L.) ecotypes. The seeds of twenty-one wild S. flexuosa (L.) Medik. ecotypes were collected from 21 sites. The edaphic and climatic characteristics of the collection sites were studied and testified to the remarkable adaptability of S. flexuosa (L.) Medik. These 21 ecotypes were cultivated in three complete randomized blocks design for two consecutive years. Statistical analysis showed substantial variability between the collected ecotypes. Principal component analysis and heatmap analysis allowed to distinguish four groups of ecotypes mainly based on nutritional parameters (fiber content and digestibility), forage production (dry matter yield, number of leaves per plant, and total number of branches), and reproduction (number of inflorescences per plant and, weight of thousand seeds and seeds per plant). Furthermore, the present study pointed out the value of ecotype 1, which was dual purpose with its high productivity, nutritional value, and reproductive parameters. Ecotype 4 was also highlighted as having late flowering but intermediate productivity, which can be used mainly for haymaking as the drying period could coincide with the last rainfall in the region.
Identifying cellular markers of focal cortical dysplasia type II with cell-type deconvolution and single-cell signatures
Authors: Galvão Isabella C. , Kandratavicius Ludmyla , Messias Lauana A. , Athié Maria C. P. , Assis-Mendonça Guilherme R. , Alvim Marina K. M. , Ghizoni Enrico , Tedeschi Helder , Yasuda Clarissa L. , Cendes Fernando , Vieira André S. , Rogerio Fabio , Lopes-Cendes Iscia , Veiga Diogo F. T.
Published: August 2023

Focal cortical dysplasia (FCD) is a brain malformation that causes medically refractory epilepsy. FCD is classified into three categories based on structural and cellular abnormalities, with FCD type II being the most common and characterized by disrupted organization of the cortex and abnormal neuronal development. In this study, we employed cell-type deconvolution and single-cell signatures to analyze bulk RNA-seq from multiple transcriptomic studies, aiming to characterize the cellular composition of brain lesions in patients with FCD IIa and IIb subtypes. Our deconvolution analyses revealed specific cellular changes in FCD IIb, including neuronal loss and an increase in reactive astrocytes (astrogliosis) when compared to FCD IIa. Astrogliosis in FCD IIb was further supported by a gene signature analysis and histologically confirmed by glial fibrillary acidic protein (GFAP) immunostaining. Overall, our findings demonstrate that FCD II subtypes exhibit differential neuronal and glial compositions, with astrogliosis emerging as a hallmark of FCD IIb. These observations, validated in independent patient cohorts and confirmed using immunohistochemistry, offer novel insights into the involvement of glial cells in FCD type II pathophysiology and may contribute to the development of targeted therapies for this condition.
Effects of transition metal carbide dispersoids on helium bubble formation in dispersion-strengthened tungsten
Authors: Saefan Ashrakat , Liu Xingyu , Lang Eric , Higgins Levko , Wang Yongqiang , El-Atwani Osman , Allain Jean Paul , Wang Xing
Published: August 2023

The formation of helium bubbles and subsequent property degradation poses a significant challenge to tungsten as a plasma-facing material in future long-pulse plasma-burning fusion reactors. In this study, we investigated helium bubble formation in dispersion-strengthened tungsten doped with transition metal carbides, including TaC, ZrC, and TiC. Of the three dispersoids, TaC exhibited the highest resistance to helium bubble formation, possibly due to the low vacancy mobility in the Group VB metal carbide and oxide phases. Under identical irradiation conditions, large helium bubbles formed at grain boundaries in tungsten, while no bubbles were observed at the interfaces between the carbide dispersoid and tungsten matrix. Moreover, our results showed the interfaces could suppress helium bubble formation in the nearby tungsten matrix, suggesting that the interfaces are more effective in trapping helium as tiny clusters. Our research provided new insights into optimizing the microstructure of dispersion-strengthened tungsten alloys to enhance their performance.
Efficacy and safety of inhaled heparin in asthmatic and chronic obstructive pulmonary disease patients: a systematic review and a meta-analysis
Authors: Ashmawy Rasha , Zaki Adel , Baess Ayman , El Sayed Iman
Published: August 2023

Asthma and chronic obstructive pulmonary disease (COPD) are prevalent chronic respiratory disorders that cause significant morbidity and mortality. Some studies evaluated the use of inhaled unfractionated heparin (UFH) in the treatment of asthma and COPD. We aimed to synthesize the available evidence for the efficacy and safety of inhaled heparin in improving lung functions among asthmatic and COPD patients. A comprehensive search was performed using Pubmed, Embase, EBSCO, Scopus, Web of Science, Cochrane CENTRAL, WHO Clinical trials, clinicaltrials.gov, Iranian Clinical trials, Google Scholar, Research Gate, ProQuest Thesis, OVID, and medRxiv databases. Two independent reviewers included all pertinent articles according to PRISMA guidelines, and extract data independently. The two reviewers checked the quality of studies using the ROB2 tool. To determine the pooled effect estimate of the efficacy and safety of inhaled heparin, a meta-analysis was carried out using the R programming language. Publication bias was evaluated using Egger’s regression test. The heterogeneity was explained using a meta-regression, and the quality of evidence was assessed by the GRADE approach. Twenty-six studies with a total of 581 patients were included in the qualitative analysis and 16 in the meta-analysis. The primary outcome was treatment success (improvement of lung function) that was measured by standardized mean differences (SMD) of the forced expiratory volume per second (FEV1) either per ml or percentage. Heparin has a large effect on both FEV1% and FEV1 ml when compared to the control group (SMD 2.7, 95% CI 1.00; 4.39; GRADE high, SMD 2.12, 95% CI − 1.49; 5.72: GRADE moderate, respectively). Secondary outcomes are other lung functions improving parameters such as PC20 (SMD 0.91, 95% CI − 0.15; 1.96). Meta-regression and subgroup analysis show that heparin type, dose, year of publication, study design, and quality of studies had a substantial effect. Regarding safety, inhaled heparin showed a good coagulation profile and mild tolerable side effects. Inhaled heparin showed improvement in lung functions either alone or when added to standard care. More large parallel RCTs are needed including COPD patients, children, and other types, and stages of asthmatic patients.
Identification and validation of lactate metabolism-related genes in oxygen-induced retinopathy
Authors: Xu Jiawei , Zhang Yunpeng , Gan Rong , Liu Zhuoqi , Deng Yan
Published: August 2023

Retinopathy of Prematurity (ROP) is a multifactorial disease characterized by abnormal retinal vascular growth in premature infants, which is one of the leading causes of childhood blindness. Lactic acid metabolism may play an imperative role in the development of ROP, but there are still few relevant studies. Our team use a dataset GSE158799 contained 284 genes in 3 P17_OIR mice and 3 P30_OIR mice to identify 41 potentially differentially expressed lactate metabolism-related genes (LMRGs) related to ROP. Then through bioinformatics analysis, we strive to reveal the interaction, the enriched pathways and the immune cell infiltration among these LMRGs, and predict their functions and internal mechanisms. These DEGs may regulate lactate metabolism, leading to the changes of metabolism and immunity, thereby inducing the development of ROP. Our results will expand our understanding of the intrinsic mechanism of ROP and may be helpful for the directions for treatment of ROP in the future.
Responses of different invasive and non-invasive ornamental plants to water stress during seed germination and vegetative growth
Authors: Mircea Diana M. , Calone Roberta , Estrelles Elena , Soriano Pilar , Sestras Radu E. , Boscaiu Monica , Sestras Adriana F. , Vicente Oscar
Published: August 2023

Biological invasions represent a major threat to natural ecosystems. A primary source of invasive plants is ornamental horticulture, which selects traits related to invasiveness. This study evaluated the responses to water stress during germination and vegetative growth of six species used as ornamental or medicinal plants. Three of them are recognised as invasive weeds in many world areas. Seeds were exposed to increasing concentrations of polyethylene glycol (PEG) mimicking drought stress, and young plants in the vegetative growth stage were subjected to two levels of water stress. Results indicated that in the absence of stress in control conditions, the most competitive species were those reported as weeds, namely Bidens pilosa L., Oenothera biennis L., and Centaurea cyanus L., the last regarding germination velocity. Under stress, only two species, Limonium sinuatum (L.) Mill. and C. cyanus, maintained germination at –1 MPa osmotic potential, but in the recovery experiment, an osmopriming effect of PEG was observed. The most tolerant species during growth were two natives in the Mediterranean region, L. sinuatum and Lobularia maritima (L.) Desv., both accumulating the highest proline concentrations. The sixth species studied, Echinacea purpurea (L.) Moench., proved to be more susceptible to stress in the two developmental stages. This study reveals that the most significant traits associated with invasiveness were related to germination, especially in the absence of stress.
Preserved perception-action integration in adolescents after a COVID-19 infection
Authors: Graf Katharina , Gustke Alena , Mösle Mariella , Armann Jakob , Schneider Josephine , Schumm Leonie , Roessner Veit , Beste Christian , Bluschke Annet
Published: August 2023

Evidence is accumulating that the Coronavirus disease (COVID-19) can bring forth deficits in executive functioning via alterations in the dopaminergic system. Importantly, dopaminergic pathways have been shown to modulate how actions and perceptions are integrated within the brain. Such alterations in event file binding could thus underlie the cognitive deficits developing after a COVID-19 infection. We examined action-perception integration in a group of young people (11–19 years of age) that had been infected with COVID-19 before study participation (n = 34) and compared them to a group of uninfected healthy controls (n = 29) on the behavioral (i.e., task accuracy, reaction time) and neurophysiological (EEG) level using an established event file binding paradigm. Groups did not differ from each other regarding demographic variables or in reporting psychiatric symptoms. Overall, multiple lines of evidence (behavioral and neurophysiological) suggest that action-perception integration is preserved in adolescents who suffered from COVID-19 prior to study participation. Event file binding processes were intact in both groups on all levels. While cognitive impairments can occur following a COVID-19 infection, the study demonstrates that action-perception integration as one of the basic building blocks of cognition seems to be largely unaffected in adolescents with a rather mild course of the disease.
Micro- and nanochamber array system for single enzyme assays
Authors: Iijima Kazuki , Kaji Noritada , Tokeshi Manabu , Baba Yoshinobu
Published: August 2023

Arrays of small reaction containers, ranging from 624 femtoliters (10–15 L) to 270 attoliters (10–18 L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic valve actuator made of polydimethylsiloxane (PDMS). The valve was actuated by PBS solution, effectively preventing evaporation of the solution from the micro- and nanochambers and allowing the assay to be performed over a long period of time. The hydrolysis rates of β-D-galactosidase (β-gal), kcat, were decreased according to the decrease of the chamber size, and the overall tendency seems to be symmetrically related to the specific surface area of the chambers even under the prevented condition of non-specific adsorption. The spatial localization of the protons in the chambers, which might could affect the dissociation state of the proteins, was also investigated to explain the decrease in the hydrolysis rate. The developed chamber system developed here may be useful for artificially reproducing the confined intracellular environment and molecular crowding conditions.