bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Study οf the Frequencies οf Atmospheric Circulation Types at Different Geopotential Levels and Their Relationship with Precipitation in Southern Romania
Authors: Tolika Konstantia , Anagnostopoulou Christina , Traboulsi Myriam , Zaharia Liliana , Constantin Dana Maria (Oprea) , Tegoulias Ioannis , Maheras Panagiotis
Published: August 2024

The primary aim of this study is to examine the characteristics of atmospheric circulation patterns at various geopotential levels and their relationship with precipitation in southern Romania during the period from 1961 to 2020. Daily geopotential heights (1000 hPa, 850 hPa, 700 hPa and 500 hPa) were utilized in an automatic updated atmospheric circulation scheme for the creation of daily calendars of 12 circulation types (5 anticyclonic and 7 cyclonic) as well as daily time series derived from five stations over the domain of interest. To assess the influence of the atmospheric circulation on precipitation, correlations and time trends were explored between the rainfall totals and the different circulation types. The findings reveal a rising trend in anticyclonic circulation types across the region, while cyclonic types exhibit a consisted decrease. Precipitation and number of rain days percentages associated with specific cyclonic types depend on the geopotential levels, while annual and seasonal precipitation linked to cyclonic types decreases progressively from higher to lower levels. The strongest correlations in circulation type frequencies are observed between adjacent circulation types. Taylor diagram analysis indicates that the relationships between circulation types and precipitation vary both seasonally and across different atmospheric levels. Notably, the two rainiest circulation types are more accurately simulated at higher atmospheric levels (700 hPa and 500 hPa).
Potential Impacts of Future Climate Change on Super-Typhoons in the Western North Pacific: Cloud-Resolving Case Studies Using Pseudo-Global Warming Experiments
Authors: Wang Chung-Chieh , Hsieh Min-Ru , Thean Yi Ting , Zheng Zhe-Wen , Huang Shin-Yi , Tsuboki Kazuhisa
Published: August 2024

Potential impacts of projected long-term climate change toward the end of the 21st century on rainfall and peak intensity of six super-typhoons in the western North Pacific (WNP) are assessed using a cloud-resolving model (CRM) and the pseudo-global warming (PGW) method, under two representative concentration pathway (RCP) emission scenarios of RCP4.5 and RCP8.5. Linear long-term trends in June–October are calculated from 38 Coupled Model Intercomparison Project phase 5 (CMIP5) models from 1981–2000 to 2081–2100, with warmings of about 3 °C in sea surface temperature, 4 °C in air temperature in the lower troposphere, and increases of 20% in moisture in RCP8.5. The changes in RCP4.5 are about half the amounts. For each typhoon, three experiments are carried out: a control run (CTL) using analysis data as initial and boundary conditions (IC/BCs), and two future runs with the trend added to the IC/BCs, one for RCP4.5 and the other for RCP8.5, respectively. Their results are compared for potential impacts of climate change. In future scenarios, all six typhoons produce more rain rather consistently, by around 10% in RCP4.5 and 20% in RCP8.5 inside 200–250 km from the center, with increased variability toward larger radii. Such increases are tested to be highly significant and can be largely explained by the increased moisture and water vapor convergence in future scenarios. However, using this method, the results on peak intensity are mixed and inconsistent, with the majority of cases becoming somewhat weaker in future runs. It is believed that in the procedure to determine the best initial time for CTL, which yielded the strongest TC, often within a few hPa in minimum central sea-level pressure to the best track data, an advantage was introduced to the CTL unintentionally. Once the long-term trends were added in future runs, the environment of the storm was altered and became not as favorable for subsequent intensification. Thus, the PGW approach may have some bias in assessing the peak intensity of such super-typhoon cases, and caution should be practiced.
Sea Fog Recognition near Coastline Using Millimeter-Wave Radar Based on Machine Learning
Authors: Li Tao , Qiu Jianhua , Xue Jianjun
Published: August 2024

Sea fog is a hazardous natural phenomenon that reduces visibility, posing a threat to ports and nearshore navigation, making the identification of nearshore sea fog crucial. Millimeter-wave radar has significant advantages over satellites in capturing sudden and localized sea fog weather. The use of millimeter-wave radar for sea fog identification is still in the exploratory stage in operational fields. Therefore, this paper proposes a nearshore sea fog identification algorithm that combines millimeter-wave radar with multiple machine learning methods. Firstly, Density-Based Spatial Clustering of Applications with Noise (DBSCAN) is used to partition radar echoes, followed by the K-means clustering algorithm (KMEANS) to divide the partitions into recognition units. Then, Sea-Fog-Recognition-Convolutional Neural Network (SFRCNN) is used to classify whether the recognition units are sea fog areas, and finally, the partition coverage algorithm is employed to improve identification accuracy. The experiments conducted using millimeter-wave radar observation data from the Pingtan Meteorological Observation Base in Fujian, China, achieved an identification accuracy of 96.94%. The results indicate that the proposed algorithm performs well and expands the application prospects of such equipment in meteorological operations.
Unusual Forbush Decreases and Geomagnetic Storms on 24 March, 2024 and 11 May, 2024
Authors: Mavromichalaki Helen , Papailiou Maria-Christina , Livada Maria , Gerontidou Maria , Paschalis Pavlos , Stassinakis Argyris , Abunina Maria , Shlyk Nataly , Abunin Artem , Belov Anatoly , Yanke Victor , Crosby Norma , Dierckxsens Mark , Drube Line
Published: August 2024

As the current solar cycle 25 progresses and moves towards solar maxima, solar activity is increasing and extreme space weather events are taking place. Two severe geomagnetic storms accompanied by two large Forbush decreases in galactic cosmic ray intensity were recorded in March and May, 2024. More precisely, on 24 March 2024, a G4 (according to the NOAA Space Weather Scale for Geomagnetic Storms) geomagnetic storm was registered, with the corresponding geomagnetic indices Kp and Dst equal to 8 and −130 nT, respectively. On the same day, the majority of ground-based neutron monitor stations recorded an unusual Forbush decrease. This event stands out from a typical Forbush decrease because of its high amplitude decrease phase and rapid recovery phase, i.e., 15% decrease and an extremely rapid recovery of 10% within 1.5 h, as recorded at the Oulu neutron monitor station. Furthermore, on 10–13 May 2024, an unusual G5 geomagnetic storm (geomagnetic indices Kp = 9 and Dst = −412 nT) was registered (the last G5 storm had been observed in 2003). In addition, the polar neutron monitor stations recorded a Ground Level Enhancement (GLE74) during the recovery phase of a large Forbush decrease of 15%, which started on 10 May 2024. In this study, a detailed analysis of these two severe events in regard to the accompanying solar activity, interplanetary conditions and solar energetic particle events is provided. Moreover, the results of the NKUA “GLE Alert++ system”, the NKUA/IZMIRAN “FD Precursory Signals” method and the NKUA “ap Prediction tool” concerning these events are presented.
Middle Ear Active Implant Indications, Comparative Audiometric Results from Different Approaches, and Coupling with the Vibrant Soundbridge®: A Single Center Experience over More Than 20 Years
Authors: Lorente-Piera Joan , Manrique-Huarte Raquel , Lima Janaina P. , Calavia Diego , Manrique Manuel
Published: August 2024

Background: Middle ear active implants, such as the Vibrant Soundbridge (VSB), offer an alternative to reconstructive surgery and other implantable hearing aid systems for patients with conductive, mixed, or sensorineural hearing loss. The primary objective of this work is to describe the auditory results obtained with VSB in our patient cohort, measuring the auditory gain in terms of average tonal thresholds and spoken word discrimination at 65 dB. Secondly, auditory gain differences between different types of hearing loss, coupling to the ossicular chain compared to round and oval windows, and the impact of open versus more conservative surgical approaches, were analyzed. Methods: A cross-sectional observational study, with retrospective data collection, was conducted at a tertiary care center. Clinical and audiometric data pre- and post-implantation were included, from patients who underwent VSB device placement surgery between 2001 and 2024. Results: 55 patients with an average age of 62.58 ± 17.83 years and a slight preference in terms of the female gender (52.72%) were included in the study. The average gain in the PTA for all types of hearing loss was 41.56 ± 22.63 dB, while for sensorineural hearing loss (SNHL) the gain was 31.04 ± 8.80 dB. For mixed-conductive hearing loss (C-MHL) a gain of 42.96 ± 17.70 was achieved, notably, in terms of absolute values, at frequencies of 4000 and 6000 Hz, with gains reaching 49.25 ± 20.26 dB at 4 K and 51.16 ± 17.48 dB at 6 K. In terms of spoken word discrimination, for all types of hearing loss, an improvement of 75.20 ± 10.11% was achieved. However, patients with C-MHL exhibited an approximately 13% higher gain compared to those with SNHL (69.32 ± 24.58% vs. 57.79 ± 15.28%). No significant differences in auditory gain were found between open and closed surgical techniques, nor in the proportion of adverse effects, when comparing one technique with the other. Conclusions: The VSB is effective in improving hearing in patients with mixed, conductive, and sensorineural hearing loss, with significant gains at high frequencies, especially through the round window membrane approach. The choice of surgical technique should consider the patient’s anatomical characteristics and specific needs in order to optimize auditory outcomes and minimize postoperative complications.
Impact of Different Wood Types on the Chemical Composition and Sensory Profile of Aged Tsipouro: A Comparative Study
Authors: Karathanos Athanassios , Soultani Georgia , Kontoudakis Nikolaos , Kotseridis Yorgos
Published: August 2024

The production of high-quality aged marc spirits includes a minimum period of six months of ageing in oak barrels. Lastly, producers are interested in alternative botanical origin wood. The present study is aimed at investigating the influence of Greek oak (Quercus trojana) and Greek chestnut (Castanea sativa) compared with French (Quercus petraia) and American oak (Quercus alba) on the chemical composition and sensorial characteristics of aged tsipouro, produced from marc from the Black Muscat variety. Gas chromatography–olfactometry–mass spectrometry (GC–O–MS) was used to identify volatile compounds of the aged tsipouro. Also, colour and polyphenol measurements were made, and an organoleptic evaluation was performed by 16 trained tasters. The findings revealed rather similar results between the tsipouro made from different wood species, especially between Greek and French oaks, and relative differentiation for that of chestnut. All the aged distillates exhibited a pleasant and rich aromatic potential, dominated by floral and fruity terpene varietal aromas as well as wood-related volatiles. Chestnut, with a high phenolic potential, gives pleasant organoleptic effects over time and can be an alternative wood for ageing spirits. This research highlights the importance of wood selection in the tsipouro ageing process and enables the use of Greek wood species in the ageing of spirits.
Comparative Study of Alternative Methods for Measuring Leg Length Discrepancy after Robot-Assisted Total Hip Arthroplasty
Authors: Nazmy Hamad , Solitro Giovanni , Domb Benjamin , Amirouche Farid
Published: August 2024

Background: Our study addresses the lack of consensus on measuring leg length discrepancy (LLD) after total hip arthroplasty (THA). We will assess the inter-observer variability and correlation between the five most commonly used LLD methods and investigate the use of trigonometric principles in overcoming the limitations of current techniques. Methods: LLD was measured on postoperative AP pelvic radiographs using five conventional methods. CT images created a 3D computer model of the pelvis and femur. The resulting models were projected onto a 2D, used to measure LLD by the five methods. The measurements were evaluated via Taguchi analysis, a statistical method identifying the process’s most influential factors. The approach was used to assess the new trigonometric method. Results: Conventional methods demonstrated poor correlation. Methods referenced to the centers of the femoral heads were insensitive to LLD originating outside the acetabular cup. Methods referencing either the inter-ischial line or the inter-obturator foramina to the lesser trochanter were sensitive to acetabular and femoral components. Trigonometry-based measurements showed a higher correlation. Conclusions: Our results underscore clinicians’ need to specify the methods used to assess LLD. Applying trigonometric principles was shown to be accurate and reliable, but it was contingent on proper radiographic alignment.
Electromyography-Triggered Constraint-Induced Movement Cycling Therapy for Enhancing Motor Function in Chronic Stroke Patients: A Randomized Controlled Trial
Authors: Park Jaemyoung , Lee Kyeongjin , Kim Junghyun , Song Changho
Published: August 2024

This single-blind randomized controlled trial investigated the effectiveness of surface electromyography (sEMG)-triggered constraint-induced movement cycling therapy (CIMCT) in improving balance, lower extremity strength, and activities of daily living in patients with chronic stroke. The participants included patients with chronic stroke-induced hemiplegia who had been diagnosed for more than 6 months, with a minimum score of 24 points on the Mini-Mental State Examination and above level 3 on the Brunnstrom stages. The trial lasted 4 weeks and participants were divided into a CIMCT group and a general cycling training (GCT) group. The CIMCT group (n = 20) used an sEMG-triggered constrained-induced movement therapy device, whereas the GCT group (n = 19) used a standard stationary bicycle. The primary outcome measures showed a significant increase in muscle strength on the affected side in the CIMCT group, as assessed by a manual muscle tester (p < 0.05), with a large effect size (d = 1.86), while no meaningful improvement was observed in the GCT group. Both groups demonstrated significant improvements in dynamic balance, as measured by the Timed Up and Go (TUG) test (p < 0.05), with the CIMCT group showing superior results compared to the GCT group, reflected by a large effect size (d = 0.96). Additionally, both groups showed significant improvements in balance as assessed by the Berg Balance Scale (BBS) and the Functional Reach Test (FRT). The CIMCT group exhibited more pronounced improvements than the GCT group, with large effect sizes of 0.83 for the BBS and 1.25 for the FRT. The secondary outcome measures revealed significant improvements in activities of daily living in both groups, as assessed by the modified Barthel index (MBI), with the CIMCT group achieving a substantial improvement (p < 0.05), accompanied by a large effect size (d = 0.87). This study concludes that sEMG-triggered CIMCT effectively improved muscle strength, postural balance, and activities of daily living in patients with chronic stroke.
Artificial Intelligence for Predicting the Aesthetic Component of the Index of Orthodontic Treatment Need
Authors: Stetzel Leah , Foucher Florence , Jang Seung Jin , Wu Tai-Hsien , Fields Henry , Schumacher Fernanda , Richmond Stephen , Ko Ching-Chang
Published: August 2024

The aesthetic component (AC) of the Index of Orthodontic Treatment Need (IOTN) is internationally recognized as a reliable and valid method for assessing aesthetic treatment need. The objective of this study is to use artificial intelligence (AI) to automate the AC assessment. A total of 1009 pre-treatment frontal intraoral photos with overjet values were collected. Each photo was graded by an experienced calibration clinician. The AI was trained using the intraoral images, overjet, and two other approaches. For Scheme 1, the training data were AC 1–10. For Scheme 2, the training data were either the two groups AC 1–5 and AC 6–10 or the three groups AC 1–4, AC 5–7, and AC 8–10. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were measured for all approaches. The performance was tested without overjet values as input. The intra-rater reliability for the grader, using kappa, was 0.84 (95% CI 0.76–0.93). Scheme 1 had 77% sensitivity, 88% specificity, 82% accuracy, 89% PPV, and 75% NPV in predicting the binary groups. All other schemes offered poor tradeoffs. Findings after omitting overjet and dataset supplementation results were mixed, depending upon perspective. We have developed deep learning-based algorithms that can predict treatment need based on IOTN-AC reference standards; this provides an adjunct to clinical assessment of dental aesthetics.
Mechanisms of Senescence and Anti-Senescence Strategies in the Skin
Authors: Konstantinou Evangelia , Longange Eliane , Kaya Gürkan
Published: August 2024

The skin is the layer of tissue that covers the largest part of the body in vertebrates, and its main function is to act as a protective barrier against external environmental factors, such as microorganisms, ultraviolet light and mechanical damage. Due to its important function, investigating the factors that lead to skin aging and age-related diseases, as well as understanding the biology of this process, is of high importance. Indeed, it has been reported that several external and internal stressors contribute to skin aging, similar to the aging of other tissues. Moreover, during aging, senescent cells accumulate in the skin and express senescence-associated factors, which act in a paracrine manner on neighboring healthy cells and tissues. In this review, we will present the factors that lead to skin aging and cellular senescence, as well as ways to study senescence in vitro and in vivo. We will further discuss the adverse effects of the accumulation of chronic senescent cells and therapeutic agents and tools to selectively target and eliminate them.
Polymorphism of Folate Metabolism Genes among Ethnic Kazakh Women with Preeclampsia in Kazakhstan: A Descriptive Study
Authors: Kaldygulova Lyazzat , Yerdessov Sauran , Ukybassova Talshyn , Kim Yevgeniy , Ayaganov Dinmukhamed , Gaiday Andrey
Published: August 2024

Introduction: Preeclampsia is a severe multifactorial complication of pregnancy. Studies found associations between folate metabolism genes’ polymorphisms and preeclampsia. However, investigations in this field are limited among Asian populations. Thus, the study’s aim was to evaluate the prevalence of methionine synthase (MTR), methionine synthase reductase (MTRR), and methylenetetrahydrofolate reductase (MTHFR) genes’ polymorphisms among ethnic Kazakh women with preeclampsia. Methods: This was a retrospective study involving 4246 patients’ data for the period of 2018–2022. Identification of MTR, MTRR, and MTHFR genes’ polymorphism was performed via PR-PCR. Peripheral blood samples were obtained for the analyses. In total, 4246 patients’ data of Kazakh ethnicity with preeclampsia at >20 weeks gestational age who had undergone an investigation to identify polymorphisms of the folate metabolism pathway genes for the period of 5 years were included in this study. Results: The most common and prevalent mutation was the MTRR A66G polymorphism: 24.5% of all tested patients with preeclampsia had the MTRR A66G polymorphism. It was highest among the 35–39 age group participants. The second most prevalent was the MTHFR C677T polymorphism: 9% of women with preeclampsia had the MTHFR C677T mutation. It was highest among women aged 30–34. There was a rare association of the MTR A2756G mutation with preeclampsia among the study participants. Conclusions: The identified levels of MTRR A66G and MTHFR C677T polymorphisms among the study participants suggest the importance of evaluating MTRR and MTHFR polymorphisms in women with preeclampsia. The role of the MTR A2756G polymorphism in the development of preeclampsia needs to be further investigated.
The Fluorinated NAD Precursors Enhance FK866 Cytotoxicity by Activating SARM1 in Glioblastoma Cells
Authors: He Wei Ming , Yang Jian Yuan , Zhao Zhi Ying , Xiao Weimin , Li Wan Hua , Zhao Yong Juan
Published: August 2024

Glioblastoma, a formidable brain tumor characterized by dysregulated NAD metabolism, poses a significant therapeutic challenge. The NAMPT inhibitor FK866, which induces NAD depletion, has shown promise in controlling tumor proliferation and modifying the tumor microenvironment. However, the clinical efficacy of FK866 as a single drug therapy for glioma is limited. In this study, we aim to disrupt NAD metabolism using fluorinated NAD precursors and explore their synergistic effect with FK866 in inducing cytotoxicity in glioblastoma cells. The synthesized analogue of nicotinamide riboside (NR), ara-F nicotinamide riboside (F-NR), inhibits nicotinamide ribose kinase (NRK) activity in vitro, reduces cellular NAD levels, and enhances FK866’s cytotoxicity in U251 glioblastoma cells, indicating a collaborative impact on cell death. Metabolic analyses reveal that F-NR undergoes conversion to fluorinated nicotinamide mononucleotide (F-NMN) and other metabolites, highlighting the intact NAD metabolic pathway in glioma cells. The activation of SARM1 by F-NMN, a potent NAD-consuming enzyme, is supported by the synergistic effect of CZ-48, a cell-permeable SARM1 activator. Temporal analysis underscores the sequential nature of events, establishing NAD depletion as a precursor to ATP depletion and eventual massive cell death. This study not only elucidates the molecular intricacies of glioblastoma cell death but also proposes a promising strategy to enhance FK866 efficacy through fluorinated NAD precursors, offering potential avenues for innovative therapeutic interventions in the challenging landscape of glioblastoma treatment.
The Putative Virulence Plasmid pYR4 of the Fish Pathogen Yersinia ruckeri Is Conjugative and Stabilized by a HigBA Toxin–Antitoxin System
Authors: Floras Fisentzos , Mawere Chantell , Singh Manvir , Wootton Victoria , Hamstead Luke , McVicker Gareth , Leo Jack C.
Published: August 2024

The bacterium Yersinia ruckeri causes enteric redmouth disease in salmonids and hence has substantial economic implications for the farmed fish industry. The Norwegian Y. ruckeri outbreak isolate NVH_3758 carries a relatively uncharacterized plasmid, pYR4, which encodes both type 4 pili and a type 4 secretion system. In this study, we demonstrate that pYR4 does not impose a growth burden on the Y. ruckeri host bacterium, nor does the plasmid contribute to twitching motility (an indicator of type 4 pilus function) or virulence in a Galleria mellonella larval model of infection. However, we show that pYR4 is conjugative. We also reveal, through mutagenesis, that pYR4 encodes a functional post-segregational killing system, HigBA, that is responsible for plasmid maintenance within Y. ruckeri. This is the first toxin–antitoxin system to be characterized for this organism. Whilst further work is needed to elucidate the virulence role of pYR4 and whether it contributes to bacterial disease under non-laboratory conditions, our results suggest that the plasmid possesses substantial stability and transfer mechanisms that imply importance within the organism. These results add to our understanding of the mobile genetic elements and evolutionary trajectory of Y. ruckeri as an important commercial pathogen, with consequences for human food production.
Inflammation and Tumor Progression: The Differential Impact of SAA in Breast Cancer Models
Authors: Olivier Daniel Wilhelm , Eksteen Carla , Plessis Manisha du , de Jager Louis , Engelbrecht Lize , McGregor Nathaniel Wade , Shridas Preetha , de Beer Frederick C. , de Villiers Willem J. S. , Pretorius Etheresia , Engelbrecht Anna-Mart
Published: August 2024

Background: Previous research has shown that the Serum Amyloid A (SAA) protein family is intricately involved in inflammatory signaling and various disease pathologies. We have previously demonstrated that SAA is associated with increased colitis disease severity and the promotion of tumorigenesis. However, the specific role of SAA proteins in breast cancer pathology remains unclear. Therefore, we investigated the role of systemic SAA1 and SAA2 (SAA1/2) in a triple-negative breast cancer mouse model. Methods: Syngeneic breast tumors were established in wild-type mice, and mice lacking the SAA1/2 (SAADKO). Subsequently, tumor volume was monitored, species survival determined, the inflammatory profiles of mice assessed with a multiplex assay, and tumor molecular biology and histology characterized with Western blotting and H&E histological staining. Results: WT tumor-bearing mice had increased levels of plasma SAA compared to wild-type control mice, while SAADKO control and tumor-bearing mice presented with lower levels of SAA in their plasma. SAADKO tumor-bearing mice also displayed significantly lower concentrations of systemic inflammatory markers. Tumors from SAADKO mice overall had lower levels of SAA compared to tumors from wild-type mice, decreased apoptosis and inflammasome signaling, and little to no tumor necrosis. Conclusions: We demonstrated that systemic SAA1/2 stimulates the activation of the NLRP3 inflammasome in breast tumors, leading to the production of pro-inflammatory cytokines. This, in turn, promoted apoptosis and tumor necrosis but did not significantly impact tumor growth or histological grading.
Transcriptome Analysis of the Effect of Nickel on Lipid Metabolism in Mouse Kidney
Authors: Zhang Jing , Gao Yahong , Li Yuewen , Liu Dongdong , Sun Wenpeng , Liu Chuncheng , Zhao Xiujuan
Published: August 2024

Although the human body needs nickel as a trace element, too much nickel exposure can be hazardous. The effects of nickel on cells include inducing oxidative stress, interfering with DNA damage repair, and altering epigenetic modifications. Glucose metabolism and lipid metabolism are closely related to oxidative stress; however, their role in nickel-induced damage needs further study. In Institute of Cancer Research (ICR) mice, our findings indicated that nickel stress increased the levels of blood lipid indicators (triglycerides, high-density lipoprotein, and cholesterol) by about 50%, blood glucose by more than two-fold, and glycated serum protein by nearly 20%. At the same time, nickel stress increased oxidative stress (malondialdehyde) and inflammation (Interleukin 6) by about 30% in the kidney. Based on next-generation sequencing technology, we detected and analyzed differentially expressed genes in the kidney caused by nickel stress. Bioinformatics analysis and experimental verification showed that nickel inhibited the expression of genes related to lipid metabolism and the AMPK and PPAR signaling pathways. The finding that nickel induces kidney injury and inhibits key genes involved in lipid metabolism and the AMPK and PPAR signaling pathways provides a theoretical basis for a deeper understanding of the mechanism of nickel-induced kidney injury.
Integrated Review of Transcriptomic and Proteomic Studies to Understand Molecular Mechanisms of Rice’s Response to Environmental Stresses
Authors: Aslam Naveed , Li Qinying , Bashir Sehrish , Yuan Liuzhen , Qiao Lei , Li Wenqiang
Published: August 2024

Rice (Oryza sativa L.) is grown nearly worldwide and is a staple food for more than half of the world’s population. With the rise in extreme weather and climate events, there is an urgent need to decode the complex mechanisms of rice’s response to environmental stress and to breed high-yield, high-quality and stress-resistant varieties. Over the past few decades, significant advancements in molecular biology have led to the widespread use of several omics methodologies to study all aspects of plant growth, development and environmental adaptation. Transcriptomics and proteomics have become the most popular techniques used to investigate plants’ stress-responsive mechanisms despite the complexity of the underlying molecular landscapes. This review offers a comprehensive and current summary of how transcriptomics and proteomics together reveal the molecular details of rice’s response to environmental stresses. It also provides a catalog of the current applications of omics in comprehending this imperative crop in relation to stress tolerance improvement and breeding. The evaluation of recent advances in CRISPR/Cas-based genome editing and the application of synthetic biology technologies highlights the possibility of expediting the development of rice cultivars that are resistant to stress and suited to various agroecological environments.
Aminopeptidasic Enzymes as Early Biomarkers of Cardiac Surgery-Associated Acute Kidney Injury and Long-Term Events
Authors: Rísquez Chica Noelia , Pereira Elisa , Manzano Francisco , Quintana María Mar Jiménez , Osuna Antonio , Fuentes María Carmen Ruiz , Wangensteen Rosemary
Published: August 2024

Background: Diagnosis of acute kidney injury (AKI) relies on serum creatinine (SCr) changes. This study investigated if urinary aminopeptidases are early and predictive biomarkers of cardiac surgery-associated AKI (CSA-AKI). Methods: Glutamyl aminopeptidase (GluAp), alanyl aminopeptidase (AlaAp), dipeptidyl peptidase-4 (DPP4), proteinuria, albuminuria, N-acetyl-β-D-glucosaminidase (NAG), and neutrophile gelatinase-associated lipocalin (NGAL) were measured in urine samples from 44 patients at arrival in the intensive care unit (ICU) after cardiac surgery. Sensitivity, specificity, and positive and negative predictive value for diagnosis of stages 1, 2, and 3 of AKI were analyzed for the highest quartile of each marker. We also studied the relationship with SCr after surgery, 6- and 12-month glomerular filtration rates (GFRs), and other long-term events over the next 5 years. Results: GluAp diagnosed the maximal number of patients that developed stage 2 or 3 of AKI, increasing diagnostic sensitivity from 0% to 75%. In addition, GluAp and DPP4 were related to the decrease in GFR at 6 or 12 months after surgery. Conclusions: Urinary aminopeptidases are a potential tool for the early diagnosis of CSA-AKI, with GluAp being the most effective marker for diagnosing stage 2 or 3 of AKI at ICU admission. GluAp and DPP4 serve as predictive biomarkers for a decrease in GFR.
Synthesis and Biological Profiling of Seven Heparin and Heparan Sulphate Analogue Trisaccharides
Authors: Demeter Fruzsina , Peleskei Zsófia , Kútvölgyi Katalin , Rusznyák Ágnes , Fenyvesi Ferenc , Kajtár Richárd , Sipos Éva , Lekli István , Molnár Petra , Szöllősi Attila Gábor , Lisztes Erika , Tóth Balázs István , Borbás Anikó , Herczeg Mihály
Published: August 2024

Researchers are paying increasing attention to the strongly negatively charged heteropolysaccharides in cells, in the extracellular matrix or in the cell wall. Examples of such molecules are glycosaminoglycans (e.g., heparin, heparan sulphate). It is well known from the literature that heparin and its derivatives have anti-inflammatory, angiogenic, metastatic and growth factor inhibitory activity. Herein, we present the efficient synthesis of six non-glycosaminoglycan (Glc-GlcA-Glc-sequenced) and one heparin-related (GlcN-GlcA-Glc-sequenced) trisaccharides with various functional group patterns. The anti-inflammatory, antioxidant and cell growth-inhibitory/cytotoxic effects of the synthesized compounds were tested. Among the investigated molecules, we have found some derivatives with a promising anti-inflammatory and antioxidant effect.
The Biological Mechanisms and Clinical Roles of RNA-Binding Proteins in Cardiovascular Diseases
Authors: Lin Lizhu , Chu Jiemei , An Sanqi , Liu Xinli , Tan Runxian
Published: August 2024

RNA-binding proteins (RBPs) have pivotal roles in cardiovascular biology, influencing various molecular mechanisms underlying cardiovascular diseases (CVDs). This review explores the significant roles of RBPs, focusing on their regulation of RNA alternative splicing, polyadenylation, and RNA editing, and their impact on CVD pathogenesis. For instance, RBPs are crucial in myocardial injury, contributing to disease progression and repair mechanisms. This review systematically analyzes the roles of RBPs in myocardial injury, arrhythmias, myocardial infarction, and heart failure, revealing intricate interactions that influence disease outcomes. Furthermore, the potential of RBPs as therapeutic targets for cardiovascular dysfunction is explored, highlighting the advances in drug development and clinical research. This review also discusses the emerging role of RBPs as biomarkers for cardiovascular diseases, offering insights into their diagnostic and prognostic potential. Despite significant progress, current research faces several limitations, which are critically examined. Finally, this review identifies the major challenges and outlines future research directions to advance the understanding and application of RBPs in cardiovascular medicine.
Advances in Immunomodulatory Mesoporous Silica Nanoparticles for Inflammatory and Cancer Therapies
Authors: Gu Bin , Zhao Qin , Ao Yiran
Published: August 2024

In recent decades, immunotherapy has been considered a promising treatment approach. The modulatable enhancement or attenuation of the body’s immune response can effectively suppress tumors. However, challenges persist in clinical applications due to the lack of precision in antigen presentation to immune cells, immune escape mechanisms, and immunotherapy-mediated side effects. As a potential delivery system for drugs and immunomodulators, mesoporous silica has attracted extensive attention recently. Mesoporous silica nanoparticles (MSNs) possess high porosity, a large specific surface area, excellent biocompatibility, and facile surface modifiability, making them suitable as multifunctional carriers in immunotherapy. This article summarizes the latest advancements in the application of MSNs as carriers in cancer immunotherapy, aiming to stimulate further exploration of the immunomodulatory mechanisms and the development of immunotherapeutics based on MSNs.
Cold Exposure Rejuvenates the Metabolic Phenotype of Panx1−/− Mice
Authors: Molica Filippo , Ehrlich Avigail , Pelli Graziano , Rusiecka Olga M. , Montessuit Christophe , Chanson Marc , Kwak Brenda R.
Published: August 2024

Pannexin1 (Panx1) ATP channels are important in adipocyte biology, potentially influencing energy storage and expenditure. We compared the metabolic phenotype of young (14 weeks old) and mature (20 weeks old) wild-type (WT) and Panx1−/− mice exposed or not to cold (6 °C) during 28 days, a condition promoting adipocyte browning. Young Panx1−/− mice weighed less and exhibited increased fat mass, improved glucose tolerance, and lower insulin sensitivity than WT mice. Their energy expenditure and respiratory exchange ratio (RER) were increased, and their fatty acid oxidation decreased. These metabolic effects were no longer observed in mature Panx1−/− mice. The exposure of mature mice to cold exacerbated their younger metabolic phenotype. The white adipose tissue (WAT) of cold-exposed Panx1−/− mice contained more small-sized adipocytes, but, in contrast to WT mice, white adipocytes did not increase their expression of Ucp1 nor of other markers of browning adipocytes. Interestingly, Glut4 expression was already enhanced in the WAT of young Panx1−/− mice kept at 22 °C as compared to WT mice. Thus, Panx1 deletion exerts overall beneficial metabolic effects in mice that are pre-adapted to chronic cold exposure. Panx1−/− mice show morphological characteristics of WAT browning, which are exacerbated upon cold exposure, an effect that appears to be associated with Ucp1-independent thermogenesis.
Cannabis sativa L. Extract Alleviates Neuropathic Pain and Modulates CB1 and CB2 Receptor Expression in Rat
Authors: Bartkowiak-Wieczorek Joanna , Bienert Agnieszka , Czora-Poczwardowska Kamila , Kujawski Radosław , Szulc Michał , Mikołajczak Przemysław , Wizner Anna-Maria , Jamka Małgorzata , Hołysz Marcin , Wielgus Karolina , Słomski Ryszard , Mądry Edyta
Published: August 2024

Introduction: Cannabis sativa L. (CSL) extract has pain-relieving potential due to its cannabinoid content, so the effects of two CSL extracts on alleviating neuropathic pain were investigated in vivo. Methods and groups: Male Wistar rats (n = 130) were divided into groups and received vincristine (0.1 mg/kg) and gabapentin (60 mg/kg) to induce and relieve neuropathic pain or CSL extracts (D and B). The mRNA and protein expression of the cannabinoid receptors type 1 and 2 (CB1R, CB2R) were evaluated in the cerebral cortex, hippocampus, and lymphocytes. Behavioural tests (Tail-Flick and von Frey) were performed on all animals. Results: VK-induced neuropathic pain was accompanied by decreased CB1R protein level and CB2R mRNA expression in the cortex. Gabapentin relieved pain and increased CB1R protein levels in the hippocampus compared to the vincristine group. Hippocampus CB1R protein expression increased with the administration of extract D (10 mg/kg, 40 mg/kg) and extract B (7.5 mg/kg, 10 mg/kg) compared to VK group. In the cerebral cortex CSL decreased CB1R protein expression (10 mg/kg, 20 mg/kg, 40 mg/kg of extract B) and mRNA level (5 mg/kg, 7.5 mg/kg of extract B; 20 mg/kg of extract D) compared to the VK-group.CB2R protein expression increased in the hippocampus after treatment with extract B (7.5 mg/kg) compared to the VK-group. In the cerebral cortex extract B (10 mg/kg, 20 mg/kg) increased CB2R protein expression compared to VK-group. Conclusion: Alterations in cannabinoid receptor expression do not fully account for the observed behavioural changes in rats. Therefore, additional signalling pathways may contribute to the initiation and transmission of neuropathic pain. The Cannabis extracts tested demonstrated antinociceptive effects comparable to gabapentin, highlighting the antinociceptive properties of Cannabis extracts for human use.
Biological Reference Intervals for 17α-Hydroxyprogesterone Immunoreactive Trypsinogen, and Biotinidase in Indian Newborns
Authors: Prasad E. Maruthi , Kinha Ramesh , Bendre Rajesh
Published: August 2024

Neonatal deaths, which usually occur in the first week after delivery, account for nearly 75 percent of all deaths of children under 5 years of age. Prematurity, birth difficulties, infections, and birth defects are responsible for about 40 percent of these deaths. Although mortality rates have declined since 2000, access to quality healthcare remains a major problem for mothers and infants worldwide. In perspective, the present study aimed to establish clear biological reference intervals for 17α-hydroxyprogesterone (17-OHP), immunoreactive trypsinogen (IRT), and biotinidase in Indian neonates. The statistical analysis of data from up to 3200 dried blood spot (DBS) samples of Indian newborns provided valuable information for the new cut-off values in newborn screening (NBS) programs. We applied correlation analysis to fix the relationship for NBS parameters such as 17-OHP, IRT, and biotinidase. This study provided important information about the distribution and comparison of key cut-offs for biomarkers considering body weights and gestational age in the Indian newborn population for the first time, which can help healthcare experts make easier treatment decisions.
Core–Shell PEDOT-PVDF Nanofiber-Based Ammonia Gas Sensor with Robust Humidity Resistance
Authors: Xiao Shenghao , Hu Mengjie , Hong Yinhui , Hu Mengjia , Sun Tongtong , Chen Dajing
Published: August 2024

Current ammonia sensors exhibit cross-sensitivity to water vapor, leading to false alarms. We developed a core–shell nanofiber (CSNF) structure to address these issues, using conductive poly(3,4-ethylenedioxythiophene) (PEDOT) as the core and hydrophobic polyvinylidene fluoride-tetrafluoroethylene (PVDF-TrFE) as the shell. The PEDOT-PVDF CSNF, with a diameter of ~500 nm and a 300 nm thick PVDF layer, showed a superior sensitivity and humidity resistance compared to conventional PEDOT membranes for ammonia concentrations of 10–100 ppm. In humid environments, CSNF sensors outperformed membrane sensors, exhibiting a tenfold increase in performance at 51% relative humidity (RH). This study highlights the potential of CSNF sensors for practical ammonia detection, maintaining a high performance under varying humidity levels.
Comparative Study of Fluorescence Emission of Fisetin, Luteolin and Quercetin Powders and Solutions: Further Evidence of the ESIPT Process
Authors: Deriabina Alexandra , Prutskij Tatiana , Morales Ochoa Hector Daniel , Delgado Curiel Esteban , Palacios Corte Veranda
Published: August 2024

Fisetin and Luteolin are important flavonoids produced in plants and known for their antioxidant, anti-inflammatory, neuroprotective, and analgesic properties. They are also good candidates for different types of biosensors. The model used to describe the fluorescence (FL) emission of these flavonoids involves an excited-state intermolecular proton transfer (ESIPT) process that causes a change in the molecule configuration and a corresponding decrease in the emission energy. Due to the different molecular structures of Fisetin and Luteolin, only one possible proton transfer within the molecule is allowed for each of them: transfer of the H3 proton for Fisetin and of the H5 for Luteolin. Here, we compare their calculated emission wavelengths, obtained using TDDFT/M06-2X/6-31++G(d,p), with their FL emission spectra measured on the corresponding powders and solutions and show that the experimental data are consistent with the presence of the ESIPT process. We also compare the emission wavelengths found for Fisetin and Luteolin with those calculated and measured for Quercetin, where, under photoexcitation, the transfers of both H3 and H5 protons are possible. We analyze the difference in the processes associated with the H3 and H5 proton transfers and discuss the reason for the predominance of the H5 proton transfer in Quercetin. Additionally, a new system of notation for flavonoid molecules is developed.
The Association between Attention-Deficit–Hyperactivity Disorder and Autistic Traits with Psychotic-like Experiences in Sample of Youths Who Were Referred to a Psychiatric Outpatient Service
Authors: Fusar-Poli Laura , Avanzato Chiara , Maccarone Giuliana , Di Martino Elide , Avincola Gabriele , Grasso Stefania , Rapisarda Giovanni , Guarnieri Francesco , Signorelli Maria Salvina
Published: August 2024

The aim of this study is to identify autism spectrum disorder (ASD) and attention-deficit–hyperactivity disorder (ADHD) traits in adolescents who experience psychotic-like experiences (PLEs), often ignored in clinical practice but widely prevalent in the general population. A total of 57 adolescents and young adults (aged between 16 and 24 years old) were recruited consecutively in the outpatient services. A total of 37 were females (64.9%), 18 were males (31.6%), and two participants (3.5%) were non-binary or gender non-conforming, with a mean age at referral of 18.26 ± 2.06. To investigate these symptoms, three sets of standardized questionnaires were used, as follows: the Autism Spectrum Quotient–short form (AQ-10), the Community Assessment of Psychic Experiences (CAPE-42), and the Adult ADHD Self-Report Scale (ASRS). We found significant associations between the ASRS and AQ-10 total scores and all CAPE scales. The model which explained the highest variance was CAPE Score’s Total Frequency score (p < 0.001). Our findings underline the importance of investigating the presence of subthreshold ASD and ADHD symptoms in clinical populations, particularly in the period of adolescence and young adulthood, to promptly identify the presence of PLEs and, thus, prevent the onset of a frank psychotic disorder, particularly in the presence of a comorbid psychopathological condition, leading to better diagnosis and treatment for individuals with two or more of these conditions.
Functional Network Connectivity for Components of Depression-Related Psychological Fragility
Authors: Evans Ian D. , Sharpley Christopher F. , Bitsika Vicki , Vessey Kirstan A. , Jesulola Emmanuel , Agnew Linda L.
Published: August 2024

Psychological resilience (PR) is known to be inversely associated with depression. While there is a growing body of research examining how depression alters activity across multiple functional neural networks, how differences in PR affect these networks is largely unexplored. This study examines the relationship between PR and functional connectivity in the alpha and beta bands within (and between) eighteen established cortical nodes in the default mode network, the central executive network, and the salience network. Resting-state EEG data from 99 adult participants (32 depressed, 67 non-depressed) were used to measure the correlation between the five factors of PR sourced from the Connor–Davidson Resilience Scale and eLORETA-based measures of coherence and phase synchronisation. Distinct functional connectivity patterns were seen across each resilience factor, with a notable absence of overlapping positive results across the depressed and non-depressed samples. These results indicate that depression may modulate how resilience is expressed in terms of fundamental neural activity.
Effect of Phasic Experimental Pain Applied during Motor Preparation or Execution on Motor Performance and Adaptation in a Reaching Task: A Randomized Trial
Authors: Badr Laïla , Gagné-Pelletier Léandre , Massé-Alarie Hugo , Mercier Catherine
Published: August 2024

Musculoskeletal conditions often involve pain related to specific movements. However, most studies on the impact of experimental pain on motor performance and learning have used tonic pain models. This study aimed to evaluate the effect of experimental phasic pain during the preparation or execution of a reaching task on the acquisition and retention of sensorimotor adaptation. Participants were divided into three groups: no pain, pain during motor preparation, and pain during motor execution. Pain was induced over the scapula with a laser while participants performed a force field adaptation task over two days. To assess the effect of pain on motor performance, two baseline conditions (with or without pain) involving unperturbed pointing movements were also conducted. The results indicated that the timing of the nociceptive stimulus differently affected baseline movement performance. Pain during motor preparation shortened reaction time, while pain during movement execution decreased task performance. However, when these baseline effects were accounted for, no impact of pain on motor adaptation or retention was observed. All groups showed significant improvements in all motor variables for both adaptation and retention. In conclusion, while acute phasic pain during motor preparation or execution can affect the movement itself, it does not interfere with motor acquisition or retention during a motor adaptation task.