bluuebunny/crossref_metadata_2025 huggingface dataset:
Absence of modulatory effects of 6Hz cerebellar transcranial alternating current stimulation on fear learning in men
Authors: Schellen Sarah Johanna , Zeidan Philip , Ernst Thomas M. , Thieme Andreas , Nicksirat Seyed Ali , Merz Christian J. , Nitsche Michael A. , Yavari Fatemeh , Timmann Dagmar , Batsikadze Giorgi
Published: January 2024

Fear is a vital defense mechanism to potential threats, which is influenced by the cerebellum. While the cerebellum’s role in acquiring fear responses is well understood, limited knowledge exists about its involvement in fear extinction. In this study, we investigated the effects of cerebellar theta band transcranial alternating current stimulation (ctACS) administered during fear extinction training, based on previous evidence from animal studies suggesting a role of cerebellar theta oscillations in associative memory formation. To this end, thirty-seven healthy right-handed male participants were recruited for a two-day differential fear renewal paradigm. On day 1, they underwent acquisition training in context A followed by extinction training in context B. On day 2, recall was tested in contexts A and B. One group of participants received ctACS in the theta band (6 Hz) during extinction training. The other group received sham ctACS. Although both groups demonstrated the ability to recall previously learned fear and distinguish between low and high threat stimuli, no significant differences were observed between the ctACS and sham groups, indicating that ctACS at this theta frequency range did not impact extinction and recall of previously acquired fear in this study. Nevertheless, using ctACS could still be useful in future research, including brain imaging studies, to better understand how the cerebellum is involved in fear and extinction processes.
A reappraisal of the default mode and frontoparietal networks in the common marmoset brain
Authors: Okuno Takuto , Ichinohe Noritaka , Woodward Alexander
Published: January 2024

In recent years the common marmoset homolog of the human default mode network (DMN) has been a hot topic of discussion in the marmoset research field. Previously, the posterior cingulate cortex regions (PGM, A19M) and posterior parietal cortex regions (LIP, MIP) were defined as the DMN, but some studies claim that these form the frontoparietal network (FPN). We restarted from a neuroanatomical point of view and identified two DMN candidates: Comp-A (which has been called both the DMN and FPN) and Comp-B. We performed GLM analysis on auditory task-fMRI and found Comp-B to be more appropriate as the DMN, and Comp-A as the FPN. Additionally, through fingerprint analysis, a DMN and FPN in the tasking human was closer to the resting common marmoset. The human DMN appears to have an advanced function that may be underdeveloped in the common marmoset brain.
Brain-behavior analysis of transcranial direct current stimulation effects on a complex surgical motor task
Authors: Walia Pushpinder , Fu Yaoyu , Norfleet Jack , Schwaitzberg Steven D. , Intes Xavier , De Suvranu , Cavuoto Lora , Dutta Anirban
Published: January 2024

Transcranial Direct Current Stimulation (tDCS) has demonstrated its potential in enhancing surgical training and performance compared to sham tDCS. However, optimizing its efficacy requires the selection of appropriate brain targets informed by neuroimaging and mechanistic understanding. Previous studies have established the feasibility of using portable brain imaging, combining functional near-infrared spectroscopy (fNIRS) with tDCS during Fundamentals of Laparoscopic Surgery (FLS) tasks. This allows concurrent monitoring of cortical activations. Building on these foundations, our study aimed to explore the multi-modal imaging of the brain response using fNIRS and electroencephalogram (EEG) to tDCS targeting the right cerebellar (CER) and left ventrolateral prefrontal cortex (PFC) during a challenging FLS suturing with intracorporeal knot tying task. Involving twelve novices with a medical/premedical background (age: 22–28 years, two males, 10 females with one female with left-hand dominance), our investigation sought mechanistic insights into tDCS effects on brain areas related to error-based learning, a fundamental skill acquisition mechanism. The results revealed that right CER tDCS applied to the posterior lobe elicited a statistically significant (q < 0.05) brain response in bilateral prefrontal areas at the onset of the FLS task, surpassing the response seen with sham tDCS. Additionally, right CER tDCS led to a significant (p < 0.05) improvement in FLS scores compared to sham tDCS. Conversely, the left PFC tDCS did not yield a statistically significant brain response or improvement in FLS performance. In conclusion, right CER tDCS demonstrated the activation of bilateral prefrontal brain areas, providing valuable mechanistic insights into the effects of CER tDCS on FLS peformance. These insights motivate future investigations into the effects of CER tDCS on error-related perception-action coupling through directed functional connectivity studies.
ALK F1174S mutation impairs ALK kinase activity in EML4-ALK variant 1 and sensitizes EML4-ALK variant 3 to crizotinib
Authors: Guan Jikui , Chuang Tzu-Po , Vikström Anders , Palmer Ruth H. , Hallberg Bengt
Published: January 2024

Objective

To assess the influence of F1174S mutation on kinase activity and drug sensitivity of the echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) fusion (EML4-ALK) variants 1 and 3.

Methods

We constructed mammalian expression plasmids of both wildtype and F1174 mutant EML4-ALK variants 1 and 3, and then characterized them with cell models by performing immunoblotting, neurite outgrowth assay, focus formation assay as well as protein stability assay. Drug sensitivity to ALK tyrosine kinase inhibitors was also compared between wildtype and F1174 mutant EML4-ALK fusions. In addition, we characterized the effect of different F1174 kinase domain mutations in the context of EML4-ALK fusions.

Results

In contrast to the oncogenic ALK-F1174S mutation that has been reported to be activating in the context of full-length ALK in neuroblastoma, EML4-ALK (F1174S) variant 1 exhibits impaired kinase activity leading to loss of oncogenicity. Furthermore, unlike the previously reported F1174C/L/V mutations, mutation of F1174 to S sensitizes EML4-ALK variants 3a and 3b to crizotinib.

Conclusion

These findings highlight the complexity of drug selection when treating patients harboring resistance mutations and suggest that the F1174S mutation in EML4-ALK variant 1 is likely not a potent oncogenic driver. Additional oncogenic driver or other resistance mechanisms should be considered in the case of EML4-ALK variant 1 with F1174S mutation.
A systematic review and meta-analysis comparing the diagnostic capability of automated breast ultrasound and contrast-enhanced ultrasound in breast cancer
Authors: Zhang Haoyu , Hu Jingyi , Meng Rong , Liu Fangfang , Xu Fan , Huang Min
Published: January 2024

Objective

To compare the diagnostic performance of automated breast ultrasound (ABUS) and contrast-enhanced ultrasound (CEUS) in breast cancer.

Methods

Published studies were collected by systematically searching the databases PubMed, Embase, Cochrane Library and Web of Science. The sensitivities, specificities, likelihood ratios and diagnostic odds ratio (DOR) were confirmed. The symmetric receiver operator characteristic curve (SROC) was used to assess the threshold of ABUS and CEUS. Fagan’s nomogram was drawn. Meta-regression and subgroup analyses were applied to search for sources of heterogeneity among the included studies.

Results

A total of 16 studies were included, comprising 4115 participants. The combined sensitivity of ABUS was 0.88 [95% CI (0.73–0.95)], specificity was 0.93 [95% CI (0.82–0.97)], area under the SROC curve (AUC) was 0.96 [95% CI (0.94–0.96)] and DOR was 89. The combined sensitivity of CEUS was 0.88 [95% CI (0.84–0.91)], specificity was 0.76 [95% CI (0.66–0.84)], AUC was 0.89 [95% CI (0.86–0.92)] and DOR was 24. The Deeks’ funnel plot showed no existing publication bias. The prospective design, partial verification bias and blinding contributed to the heterogeneity in specificity, while no sources contributed to the heterogeneity in sensitivity. The post-test probability of ABUS in BC was 75%, and the post-test probability of CEUS in breast cancer was 48%.

Conclusion

Compared with CEUS, ABUS showed higher specificity and DOR for detecting breast cancer. ABUS is expected to further improve the accuracy of BC diagnosis.
The association between graded prognostic assessment and the prognosis of brain metastases after whole brain radiotherapy: a meta-analysis
Authors: Geng Xiaohan , Kou Changgui , Wang Jianfeng
Published: January 2024

Introduction

This meta-analysis aims to provide evidence-based medical evidence for formulating rational treatment strategies and evaluating the prognosis of brain metastasis (BM) patients by assessing the effectiveness of the graded prognostic assessment (GPA) model in predicting the survival prognosis of patients with BM after whole-brain radiotherapy (WBRT).

Methods

A comprehensive search was conducted in multiple databases, including the China Biomedical Literature Database (CBM), China National Knowledge Infrastructure (CNKI), PubMed, Wanfang database, Cochrane Library, Web of Science, and Embase. Cohort studies that met the inclusion and exclusion criteria were selected. The quality of the included literature was evaluated using the Newcastle-Ottawa Scale, and all statistical analyses were performed with R version 4.2.2. The effect size (ES) was measured by the hazard ratio (HR) of overall survival (OS). The OS rates at 3, 6, 12, and 24 months of patients with BM were compared between those with GPAs of 1.5–2.5, 3.0, and 3.5–4.0 and those with GPAs of 0–1 after WBRT.

Results

A total of 1,797 participants who underwent WBRT were included in this study. The meta-analysis revealed a significant association between GPA and OS rates after WBRT: compared with BM patients with GPA of 0–1, 3-month OS rates after WBRT were significantly higher in BM patients with GPA of 1.5–2.5 (HR = 0.48; 95% CI: 0.40–0.59), GPA of 3 (HR = 0.38; 95% CI: 0.25–0.57), and GPA of 3.5–4 (HR = 0.28; 95% CI: 0.15–0.52); 6-month OS rates after WBRT were significantly higher in BM patients with GPA of 1.5–2.5 (HR = 0.48; 95% CI: 0.41–0.56), GPA of 3 (HR = 0.33; 95% CI: 0.24–0.45), and GPA of 3.5–4 (HR = 0.24; 95% CI: 0.16–0.35); 12-month OS rates after WBRT were significantly higher in BM patients with GPA of 1.5–2.5 (HR = 0.49; 95% CI: 0.41–0.58), GPA of 3 (HR = 0.48; 95% CI: 0.32–0.73), and GPA of 3.5–4 (HR = 0.31; 95% CI: 0.12–0.79); and 24-month OS rates after WBRT were significantly higher in BM patients with GPA of 1.5–2.5 (HR = 0.49; 95% CI: 0.42–0.58), GPA of 3 (HR = 0.49; 95% CI: 0.32–0.74), and GPA of 3.5–4 (HR = 0.38; 95% CI: 0.15–0.94).

Conclusion

BM patients with higher GPAs generally exhibited better prognoses and survival outcomes after WBRT compared to those with lower GPAs.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42023422914.
Jaundice-predominant manifestation of Kawasaki disease in children
Authors: Huang Ya-Ning , Lin Chien-Yu , Chi Hsin , Chiu Nan-Chang , Huang Daniel Tsung-Ning , Chang Lung , Kung Yen-Hsin , Huang Ching-Ying
Published: January 2024

Background

A jaundice-predominant presentation of Kawasaki disease (KD) is atypical.

Methods

A total of 12 children with KD with a predominant manifestation of jaundice at MacKay Children's Hospital were reviewed, along with 42 cases reported in the literature since 1990.

Results

The median age of the 12 patients was 1.85 years (range: 3 months–4 years), and 66.6% were male. All of the patients had elevated liver function at presentation, 50% had hydrops of the gallbladder, and almost 60% had gastrointestinal symptoms and signs. Complete KD was evident in 11 of the 12 patients (91.7%), and two patients (16.7%) had recurrent episodes. All of the patients received intravenous immunoglobulin (IVIG); however, one-third were refractory to treatment. Corticosteroids were used in five (41.7%) of the patients. Three (25%) of the patients had shock, and seven (58.3%) had coronary artery abnormalities, of whom one (8.3%) had persistent coronary artery aneurysm and the others recovered. A review of the 42 cases in the literature showed that the children with a jaundice-predominant presentation of KD had high rates of IVIG-refractory disease (25%), coronary artery abnormalities (25%), shock (13.2%), and corticosteroid treatment (24.2%).

Conclusions

Children with KD presenting with a jaundice-predominant manifestation are at a higher risk of IVIG-refractory disease, coronary artery abnormalities, and more recurrent episodes. Physicians should be aware of the risk of shock in this population.
Case Report: Neonatal genital prolapse in a pair of premature Twins
Authors: Gu Jian , Chen Xiaochun , Li Shikun , Chen Wenliang , Zhong Xinqi
Published: January 2024

Neonatal genital prolapse is a rare situation. This report presents a unique case involving a pair of premature female twins who both developed vaginal wall prolapse without any neurological deficits. Multiple factors such as selective intrauterine growth restriction, feeding intolerance, extrauterine growth retardation, and elevated intra-abdominal pressure after birth may have contributed to the development of this phenomenon. Notably, the severity of prolapse was more pronounced in the twin with lower birth weight and smaller for gestational age. After a five-month follow-up period, the twins’ prolapsed vaginal wall fully retracted due to a combination of conservative treatment and enhanced nutritional support.
Prophylactic infusion of norepinephrine does not affect the rostral spread of spinal anesthesia in pregnancy: a prospective, randomized, double-blinded study
Authors: Dong Yu-Fang , Qian Jing , Wang Jing , Wang Li-Zhong , Qian Xing-Hua , Xiao Fei
Published: January 2024

Background: The infusion of phenylephrine to prevent spinal-induced hypotension (SIH) in cesarean delivery may decrease the rostral spread of a spinal local anesthetic. We hypothesized that infusion of norepinephrine may decrease the rostral spread of spinal anesthesia, similar to that caused by phenylephrine. The aim of this study was to compare the block height of spinal anesthesia in the presence or absence of norepinephrine infusion administered to prevent SIH during cesarean delivery.

Methods: Eighty patients were enrolled and allocated into groups receiving a norepinephrine infusion (group N) or saline infusion (group C). After intrathecal injection of hyperbaric bupivacaine 10 mg, the block height for cold and pinprick sensation was checked 10 and 20 min after the injection. The demographic characteristics, spinal anesthesia, side effects, and neonatal outcomes were also recorded.

Results: The block height for cold and pinprick sensation was similar between the two groups, although the incidence of hypotension was significantly lower (p < 0.00) in group N than in group C. Systolic blood pressure was also more stable in group N than in group C, with the incidence of interventions being significantly lower in group N. There was no significant difference in patient satisfaction between the two groups.

Conclusion: Evidence from this study suggested that prophylactic norepinephrine infusion does not reduce the rostral spread of spinal anesthesia in pregnancy. We suggest that it is not necessary to increase the dose of an intrathecal local anesthetic for cesarean delivery when prophylactic norepinephrine is administered.

Clinical Trial Registration: https://www.chictr.org.cn/bin/project/edit?pid=152899, identifier [ChiCTR2200057439].
Comparative analyses of complete chloroplast genomes reveal interspecific difference and intraspecific variation of Tripterygium genus
Authors: Xu Kai-Ling , Zhang Zhong-Mou , Fang Wen-Liang , Wang Ya-Dan , Jin Hong-Yu , Wei Feng , Ma Shuang-Cheng
Published: January 2024

The genus Tripterygium was of great medicinal value and attracted much attention on the taxonomic study using morphological and molecular methods. In this study, we assembled 12 chloroplast genomes of Tripterygium to reveal interspecific difference and intraspecific variation. The sequence length (156,692–157,061 bp) and structure of Tripterygium were conserved. Comparative analyses presented abundant variable regions for further study. Meanwhile, we determined the ndhB gene under positive selection through adaptive evolution analysis. And the phylogenetic analyses based on 15 chloroplast genomes supported the monophyly of Tripterygium hypoglaucum and the potential sister relationship between Tripterygium wilfordii and Tripterygium regelii. Molecular dating analysis indicated that the divergence time within Tripterygium was approximately 5.99 Ma (95% HPD = 3.11–8.68 Ma). The results in our study provided new insights into the taxonomy, evolution process, and phylogenetic construction of Tripterygium using complete plastid genomes.
Multi-omics combined with MALDI mass spectroscopy imaging reveals the mechanisms of biosynthesis of characteristic compounds in Tetrastigma hemsleyanum Diels et Gilg
Authors: Lin Yan , Jiang Xuechun , Zhu Sheng , Dun Junling , Pu Jinbao , Liang Weiqing
Published: January 2024

Tetrastigma hemsleyanum Diels et Gilg is recognized as a source of extracts with various desirable bioactivities. However, current knowledge regarding the mechanisms of biosynthesis of flavonoids, phenolic compounds, and other bioactive chemicals is limited. We conducted comprehensive tissue distribution studies and biosynthetic analyses of the 26 main bioactive compounds of this plant. The majority of flavonoids exhibited higher concentrations in the cortex (CT) compared to the vascular cylinder (VC). The expression levels of genes and proteins in CT and VC were quantified using mRNA sequencing and isobaric tags for relative and absolute quantification (iTRAQ). A total of 31,700 genes were identified, among which 4921 exhibited differential expression between CT and VC. A total of 13,996 proteins were identified in the proteomes of CT and VC, with 927 showing differential expression. Co-expression network analyses of DEGs and DEPs from multiple sites demonstrated substantial pathway variations linked to flavonoid biosynthesis. Through differential enrichment analysis, a total of 32 genes involved in the flavone biosynthesis pathway were identified, with iTRAQ specifically detecting C3’H, F3H and FLS. Pearson correlation analysis revealed a strong association between the expression levels of C3’H, F3H, and FLS and the concentrations of flavonoids. The validation of multiple genes encoding pivotal enzymes was conducted using real-time fluorescence quantitative PCR (RT-qPCR). The findings provide a foundation for future investigations into the molecular mechanisms and functional characterization of T. hemsleyanum candidate genes associated with characteristic compounds.
Gender roles and political ideology in the pandemic: experimental evidence from Western Europe
Authors: Lesschaeve Christophe , Glaurdić Josip , D'Ambrosio Conchita , Vögele Claus
Published: January 2024

The economic shutdown and national lockdown following the outbreak of COVID-19 forced families to take on tasks themselves that were previously outsourced, like child care and housecleaning. These tasks were, and to a degree still are, traditionally performed by women. The concern is that the pandemic placed these burdens again primarily on their shoulders. In this study, we examine how the lockdown-imposed difficulties to the outsourcing of essential household tasks affected views on who in the family should sacrifice their career to cope with new challenges, and how these views interacted with ideological commitments. Analyzing data collected from an experiment embedded in a representative survey of nearly 4,000 residents from five West European countries, we find that the pandemic reduced the ideological polarization between the political left and right with regards to gender roles and household tasks. However, this reduced polarization is primarily found among female respondents.
Delivery of pleasant stroke touch via robot in older adults
Authors: Ishikura Tomoki , Sato Wataru , Takamatsu Jun , Yuguchi Akishige , Cho Sung-Gwi , Ding Ming , Yoshikawa Sakiko , Ogasawara Tsukasa
Published: January 2024

Touch care has clinically positive effects on older adults. Touch can be delivered using robots, addressing the lack of caregivers. A recent study of younger participants showed that stroke touch delivered via robot produced subjective and physiologically positive emotional responses similar to those evoked by human touch. However, whether robotic touch can elicit similar responses in older adults remains unknown. We investigated this topic by assessing subjective rating (valence and arousal) and physiological signals [corrugator and zygomatic electromyography (EMG) and skin conductance response (SCR)] to gentle stroking motions delivered to the backs of older participants by robot and human agents at two different speeds: 2.6 and 8.5 cm/s. Following the recent study, the participants were informed that only the robot strokes them. We compared the difference between the younger (their data from the previous study) and the older participants in their responses when the two agents (a robot and a human) stroked them. Subjectively, data from both younger and older participants showed that 8.5 cm/s stroking was more positive and arousing than 2.6 cm/s stroking for both human and robot agents. Physiologically, data from both younger and older participants showed that 8.5 cm/s stroking induced weaker corrugator EMG activity and stronger SCR activity than the 2.6 cm/s stroking for both agents. These results demonstrate that the overall patterns of the older groups responses were similar to those of the younger group, and suggest that robot-delivered stroke touch can elicit pleasant emotional responses in older adults.
The impact of the COVID-19 pandemic on academic performance: a comparative analysis of face-to face and online assessment
Authors: Vicario Carmelo Mario , Mucciardi Massimo , Perconti Pietro , Lucifora Chiara , Nitsche Michael A. , Avenanti Alessio
Published: January 2024

Introduction

Survey studies yield mixed results on the influence of the COVID-19 pandemic on academic performance, with limited direct evidence available.

Methodology

Using the academic platform from the Italian university system, a large-scale archival study involving 30,731 students and 829 examiners encompassing a total of 246,416 exams (oral tests only) to scrutinize the influence of the COVID-19 pandemic on the likelihood of passing exams was conducted. Examination data were collected both in face-to-face and online formats during the pandemic. In the pre-pandemic period, only face-to-face data were accessible.

Results

In face-to-face examination, we observed a lower probability of passing exams during the pandemic as opposed to pre-pandemic periods. Notably, during the pandemic we found an increased chance of passing exams conducted through online platforms compared to face-to-face assessments.

Discussion and conclusions

These findings provide the first direct evidence of an adverse impact of the COVID-19 pandemic on academic performance. Furthermore, the results align with prior survey studies underscoring that using telematics platforms to evaluate students' performance increases the probability of exam success. This research significantly contributes to ongoing efforts aimed to comprehend how lockdowns and the widespread use of online platforms impact academic assessment processes.
Adult attention-deficit/hyperactivity symptoms and parental cognitions: a meta-analysis
Authors: Miklósi Mónika , Kovács Barbara , Janovicz Júlia , Lelki Franciska , Kassai Réka
Published: January 2024

Introduction

Attention-deficit/hyperactivity disorder (ADHD) symptoms in adults interfere with parental functioning. Dysfunctional parental cognitions may play a role in this impairment. Despite the importance of parental cognitions on parents and children’s outcomes, up to now, no systematic review or meta-analysis of these findings is available. To fill this gap, this meta-analysis aimed to evaluate the relationship between adult ADHD symptoms and parental cognitions.

Methods

We conducted searches in Web of Science, PubMed, and ProQuest from January 2000 to June 2023. Studies were included if they provided data on the relationship between parental ADHD symptoms and parental cognitions by means of a row correlational coefficient, or means and standard deviation were reported for each study group. A random-effects model was used. Publication bias was assessed by funnel plot and Rosenthal’s fail-safe N. Moderator analyses were conducted by means of subgroup analysis and meta-regression analyses.

Results

Fifteen published papers were included (N = 2851), and 51 effect sizes were analysed. The weighted mean effect size was small but significant (Fisher’s Z = 0.186, k = 15, 95% CI [0.120 – 0.252], z = 5.539, p < 0.001), indicating that ADHD symptoms in adults are associated with more negative and less positive parental cognitions. The Fail-Safe N analysis suggested a robust effect. Tweedie’s trim and fill results suggested that five studies were missing; after five missing studies had been imputed, the mean overall effect size dropped to 0.116 (0.080 – 0.152). There was significant heterogeneity among effect sizes. The methodology of the study was found to be a significant moderator. Meta-regression analyses revealed that the lower age of the parent and the child were related to more negative parental cognitions.

Discussion

Though the analysis might be inflated by publication bias, our results suggest a significant association between ADHD symptom level and dysfunctional parental cognitions. Biased negative perceptions of the parental role, the child and co-parenting may play a central mediator role between parental ADHD and parent and child outcomes. Given the familiar nature of ADHD, targeting dysfunctional parental cognitions in parent training programs is warranted.

Systematic review registration

osf.io/pnur7.
Combining legal epidemiology and implementation science to improve global access to medicines: challenges and opportunities
Authors: Lane Jeff , Stergachis Andy
Published: January 2024

Laws and policies affecting access to medicines have been in the global health spotlight for decades, yet our understanding of their effects remains substantially underdeveloped. The emerging field of legal epidemiology combined with the methods of implementation science presents an opportunity to help address this gap. Legal epidemiology refers to the scientific study and deployment of law as a factor in the cause, distribution, and prevention of disease and injury in a population. Legal epidemiology studies consist of a systematic collection and coding of laws and policies relating to a particular topic. Quasi-experimental or observational research methods can then be applied to take advantage of natural experiments resulting from heterogenous adoption and/or implementation of laws and policies. Often legal epidemiology studies fail to account for heterogenous law implementation processes, presenting a need and opportunity to integrate implementation science methods. Researchers may face challenges in integrating these methods for access to medicines studies, including data access issues and a complex legal and implementation environment. Yet, the opportunities presented by increasingly transparent legal environments, improved monitoring of medicine availability, universal health coverage expansion, and electronic health and insurance records integration may facilitate overcoming these challenges. Improved collaboration and communication between researchers, health authorities, manufacturers, and health providers from public and private sectors will be critical. In spite of the challenges, combining the fields of legal epidemiology and implementation science may present an important strategy toward creating a legal and policy environment that supports global and equitable access to medicines.
Sound masking by a low-pitch speech-shaped noise improves a social robot’s talk in noisy environments
Authors: Pourfannan Hamed , Mahzoon Hamed , Yoshikawa Yuichiro , Ishiguro Hiroshi
Published: January 2024

Introduction: There has been a surge in the use of social robots for providing information, persuasion, and entertainment in noisy public spaces in recent years. Considering the well-documented negative effect of noise on human cognition, masking sounds have been introduced. Masking sounds work, in principle, by making the intrusive background speeches less intelligible, and hence, less distracting. However, this reduced distraction comes with the cost of increasing annoyance and reduced cognitive performance in the users of masking sounds.

Methods: In a previous study, it was shown that reducing the fundamental frequency of the speech-shaped noise as a masking sound significantly contributes to its being less annoying and more efficient. In this study, the effectiveness of the proposed masking sound was tested on the performance of subjects listening to a lecture given by a social robot in a noisy cocktail party environment.

Results: The results indicate that the presence of the masking sound significantly increased speech comprehension, perceived understandability, acoustic satisfaction, and sound privacy of the individuals listening to the robot in an adverse listening condition.

Discussion: To the knowledge of the authors, no previous work has investigated the application of sound masking technology in human-robot interaction designs. The future directions of this trend are discussed.
The combinatorial effect of age and biological sex on alloimmunity and transplantation outcome
Authors: Martin Friederike , Xiao Yao , Welten Vanessa , Nakamori Keita , Gizlenci Merih , Zhou Hao , Tullius Stefan G.
Published: January 2024

Both age and biological sex affect transplantation outcomes. We have recently shown in a large volume clinical analysis utilizing the SRTR data that graft survival is inferior in young female kidney transplant recipients. In this multi-factorial analysis, older female recipients presented with a trend towards improved transplant outcomes compared to both young female recipients and male recipients of any age. Those data supported by reports of those of others suggest that sex and age impact alloimmune responses both, individually and synergistically. Biological sex and hormone levels change throughout a lifetime with recognized effects on longevity in addition to an impact on the development and course of several disease preconditions. Detailed mechanisms of those sex and age-specific aspects have thus far been studied outside of transplantation. Effects on alloimmunity are largely unknown. Moreover, the combinatorial impact that both, biological sex and age have on transplant outcomes is not understood. Here, we summarize available data that analyze how age in combination with biological sex may shape alloimmune responses and affect transplant outcomes.
The Importance of Mussels for the Planet
Authors: Souza Helena de Oliveira , Ferreira Vanessa de Magalhães , Moraes William dos Santos , Fernandez Marcos Antônio dos Santos
Published: January 2024

Here is a riddle: it has a soft body, no head, and lives inside two shells, clinging to the rocks in the sea… what kind of animal is it? If you said mussel, you got it! Mussels are constantly working as they filter the seawater in search of food, and filtering makes the water healthier for the other organisms that live there. Along with their role in keeping the environment healthy, mussels are also important for humans—as a source of income for some and a source of food for many. Unfortunately, global warming and human-caused pollution are threatening the lives of mussels and putting the entire ocean environment at risk. There are a few simple things we can all do to protect mussels—but people usually do not take care of things they do not understand, do you agree? So, keep reading to learn about mussels and what you can do to help them!
How Might Plastic Pollution Affect Antarctic Animals?
Authors: Rowlands Emily , Galloway Tamara , Jones-Williams Kirstie , Manno Clara
Published: January 2024

Antarctica is the least populated place on Earth, but the frozen continent and its surrounding Southern Ocean are still affected by human activities. Scientists have found large pieces of plastic such as fishing nets, and microscopic-sized pieces of plastic, too. Some plastics can be hundreds of times smaller than a grain of sand, and these are called nanoplastics. The Southern Ocean, which surrounds Antarctica, is also warming faster than other oceans and is becoming more acidic. Thus, Antarctic marine animals that have lived in an untouched, stable environment for millions of years are now being exposed to plastic pollution and human-caused climate change. We are studying how Antarctic marine life is coping with plastic pollution in this quickly changing ocean. We focus on Antarctic krill, a small crustacean that supports the Antarctic marine food web. Findings show that krill embryos subjected to ocean acidification and nanoplastics develop less than embryos in ordinary seawater conditions.
Wait! How We Control Our Thoughts and Actions as We Age
Authors: Grégoire Coline , Majerus Steve
Published: January 2024

Have you ever sat next to someone in class who cannot stop talking? You would like to focus on your teacher, but you cannot avoid listening to your talkative classmate. This is what inhibition is useful for! Inhibition is the ability to ignore, suppress, and resist irrelevant information coming from the environment or from our own minds. Inhibition is more difficult for young kids, is optimal in young adults, and becomes more problematic as we age. How does inhibition work? Why does inhibition change as we age? Can we train inhibition? In this article, we will try to answer these questions.
Salmonella: A Star Among Disease-Causing Bacteria Transmitted by Food
Authors: Vilela Felipe Pinheiro , Falcão Juliana Pfrimer
Published: January 2024

Salmonella is an important type of bacteria capable of infecting humans and animals. These bacteria were discovered over 100 years ago, and thousands of types have now been identified. Salmonella can be found in many sources, and its pathway to causing disease in humans is a complex web. Salmonella can live in farm, wild, and domesticated animals; the environment; and water sources—all of which can contaminate food items and, in consequence, humans. The most frequent illness in humans is called salmonellosis, which is characterized by diarrhea, stomach cramps, fever, nausea, vomiting, and headache. Although most people have mild symptoms, a few cases may evolve into a more severe illness that may need medicines called antibiotics to treat. In this article, you will learn about the importance of Salmonella, as well as the challenges it poses for the future and promising scientific advances that will improve our knowledge of these bacteria.
How Do Plants Protect Themselves Against Animal Attacks?
Authors: Ochoa Alvarez Tamara Citlali , González Rodríguez Antonio
Published: January 2024

Natural systems are made up of countless interactions between living beings. One of the most essential interactions is when a plant is eaten by an animal. Why is this so important? When plants are eaten, their growth and ability to reproduce decrease, and we all know that plants are critical because they provide us with food, medicine, and wood. They also provide homes for other living things, produce the oxygen we breathe, keep soils healthy, regulate humidity, and contribute to climate stability. Plants have also developed smart strategies to defend themselves from animal attacks. Some plants have evolved protective structures like thorns and thick leaves, while others have produced defensive chemicals. Together, plant protection mechanisms can reduce or prevent the damage that plant predators may cause. In this article, you will learn many strategies plants evolved to protect themselves from plant-eating animals.
Application of collisional analysis to the differential velocity of solar wind ions
Authors: Johnson E. , Maruca B. A. , McManus M. , Stevens M. , Klein K. G. , Mostafavi P.
Published: January 2024

Collisional analysis combines the effects of collisional relaxation and large-scale expansion to quantify how solar wind parameters evolve as the plasma expands through the heliosphere. Though previous studies have applied collisional analysis to the temperature ratio between protons (ionized hydrogen) and α-particles (fully ionized helium), this is the first study to explore α-proton differential flow with collisional analysis. First, the mathematical model for the collisional analysis of differential flow was derived. Then, this model was applied to individual in-situ observations from Parker Solar Probe (PSP; r = 0.1–0.27 au) to generate predictions of the α-proton differential flow in the near-Earth solar wind. A comparison of these predicted values with contemporaneous measurements from the Wind spacecraft (r = 1.0 au) shows strong agreement, which may imply that the effects of expansion and Coulomb collisions have a large role in governing the evolution of differential flow through the inner heliosphere.
Extraction of proteinaceous components and biominerals from cold water fish fileting side streams: a review
Authors: Kendler Sophie , Sasidharan Abhilash , Rustad Turid
Published: January 2024

Fileting is a popular form of processing methods and in addition to being sold fresh or frozen, filets are used for preparation products battered and breaded filets. This generates considerable amount of side streams like skin, frames, and cut-offs which forms around 30–70% of the total body weight. The European Waste Framework Directive 2008 and recent amendments [EU WFD (2018/851)] stipulates comprehensive regulations which the manufacturers must follow while handling the side streams generated during the processing. This demands a detailed compilation of information regarding the yield, classification and valorization potential of side streams associated with the fileting operations of the cold-water finfishes. The side streams are a rich source of proteins including structural proteins like collagen and biominerals such as hydroxyapatite which find multiple application in food and pharmaceutical industry. Previously, the recovery of these components was performed by extensive chemical treatment with acids or bases, including subsequent washing steps. Nowadays, green extraction methods, defined as technologies with reduced energy and chemical consumption, should be considered to achieve a green shift in the food industry. Nevertheless, industrial upscaling of green extraction methods and subsequent refinement of the isolated compounds must be further evaluated and improved in order to achieve a green shift in food industry by using side-stream derived compounds as ingredients. Moreover, legislations as well as national and international regulations must be considered and evaluated. Even though a number of articles are recently available regarding seafood side stream valorization, this review focus on side streams generated predominantly from cold water fish species and also discusses sustainable green technologies to be included during the recovery process.
Surgical anatomy and clinical variation of the left colonic artery in laparoscopic anterior rectal resection
Authors: Li Jiajun , Zhao Xiaofang , Yi Bo , Fu Chuanchuan , Xu Peipei , Chen Chao , Zhao Bin , Zheng Yangchun
Published: January 2024

Objectives

This study aims to investigate the surgical anatomy and clinical variation of the left colonic artery (LCA) during laparoscopic anterior rectal resection.

Methods

We conducted a retrospective analysis of 87 patients diagnosed with colorectal cancer who underwent laparoscopic anterior rectal resection with preserved LCA at the Department of Gastroenterology, Sichuan Cancer Hospital, between March 2018 and April 2022, aiming to observe the emanation location, anatomical typing, and travel trajectory of the LCA, as well as its relationship with the inferior mesenteric vein (IMV).

Results

In all observed cases, we observed that the LCA emanated from the left side of the inferior mesenteric artery (IMA), and the average distance from the root of the IMA to the emanation of the LCA was approximately 3.5 ± 1.1 cm. Specifically, 35 of these cases had the LCA branching from the IMA alone (Type I, 40.2%),16 cases had the LCA cotruncating with the sigmoid artery (SA) (Type II, 18.4%), 30 cases had the LCA cotruncating with the superior rectal artery (SRA) and SA (Type III, 34.5%), and six cases had the LCA cotruncating with four or more branches of the SRA and SA (Type IV, 6.9%). No LCA agenesis cases were found in this group. In addition, we also observed the occurrence of LCA alignment. Specifically, there were 25 cases where the LCA crossed the IMV in a diagonal upward direction (Type A, 28.7%), 36 cases where the LCA crossed the IMV in an upward arched manner (Type B, 41.4%), 18 cases where the LCA crossed the IMV in a vertical outward direction (Type C, 20.7%), and eight cases where the LCA crossed the IMV in a diagonal downward manner (Type D, 9.2%). Among them, two cases developed anastomotic fistula, one case had chyle leakage 1 week after surgery, and four cases experienced urinary retention; all of the patients successfully recovered and were discharged after receiving conservative treatment.

Conclusion

The anatomy and variation of the LCA can be clearly and accurately observed during laparoscopic surgery. Understanding the type and variation of the LCA helps to dissect the vessels in the IMA region during surgery, particularly in cases when the LCA is preserved, and reduce the incidence of vascular injury and its complications.
Effect of feeding a dried distillers’ grains with solubles diet on the metabolism of the intestinal wall in Guanling crossbred cattle: a preliminary assessment
Authors: Luo Xiaofen , Zhang Tiantian , Xu Duhan , Zhu Mingming , Zhang Junjie , Zhang Rong , He Guangxia , Chen Ze , Mei Shihui , Zhou Bijun , Wang Kaigong , Chen Chao , Zhu Erpeng , Cheng Zhentao
Published: January 2024

Dried distillers’ grains with solubles (DDGS)-based diets are nutritious and can improve the inflammations and intestinal immunity in livestock. However, there is limited research examining the effect of feeding DDGS-based diets on changes in intestinal metabolites and related pathways in livestock. In this study, six Guanling crossbred cattle (Guizhou Guanling Yellow cattle × Simmental cattle) were selected and divided into a basal diet (BD) group and an experimental group fed with DDGS replacing 25% of the daily ration concentrates (DDGS) (n=3), respectively. Fresh jejunum (J), ileum (I) and cecum (C) tissues were collected for metabolomic analysis. Differential metabolites and metabolic pathways were explored by means of univariate and multivariate statistical analysis. In comparison to the J-BD group, 123 differential metabolites (VIP > 1, p < 0.05) were identified in the J-DDGS group, which (top 20) were mainly divided into superclasses, including lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic compounds. Compared with the I-BD group, 47 differential metabolites were obtained in the I-DDGS group, which were mainly divided into superclasses, including lipids and lipid-like molecules and organic acids and derivatives. The C-DDGS vs. C-BD comparison revealed 88 differential metabolites, which were mainly divided into superclasses, including lipids and lipid-like molecules, organic oxygen compounds, and nucleosides. A total of 34 significant metabolic pathways were found (p < 0.05, −log(p) > 1.3). Among them, 3 significant pathways were significantly enriched in the J-DDGS group, 11 significant pathways were significantly enriched in the I-DDGS group, and 20 significant pathways were significantly enriched in the C-DDGS group. Importantly, primary bile acid biosynthesis, linoleic acid metabolism, and arachidonic acid metabolism correlated with intestinal inflammation and immunity by regulating gut microbiota, prostaglandin synthesis, and cell signaling. The data suggest that DDGS-fed cattle unregulated three metabolic pathways mentioned above and that a DDGS-based diet was able to maintain a balance of these three metabolic pathways, thus resulting in improvement of intestinal inflammation and enhanced immunity in cattle. In conclusion, the DDGS diet has the potential to improve intestinal inflammation and enhance the immunity of Guanling crossbred cattle by regulating the metabolic patterns of lipids and lipid-like molecules, organic acids and derivatives, and related metabolic pathways. These results allude to potential metabolic regulatory mechanisms of DDGS diets and also provide a theoretical basis for the application of DDGS in livestock feed.
Drug resistance patterns and genotype associations of Trichomonas gallinae in meat pigeons (Columba livia): insights from Guangdong Province, China
Authors: Cai Haiming , Liu Yu , Zhu Yibin , Fang Siyun , Wang Dingai , Yan Zhuanqiang , Shen Hanqin , Liao Shenquan , Qi Nanshan , Li Juan , Lin Xuhui , Hu Junjing , Song Yongle , Chen Xiangjie , Yin Lijun , Zhang Jianfei , Lv Minna , Sun Mingfei
Published: January 2024

Avian trichomoniasis, caused by the protozoan parasite Trichomonas gallinae, is a prevalent and economically significant disease in pigeons. This study investigated the drug resistance of T. gallinae isolates in Guangdong Province, China. The results revealed that 25.3% (20/79) of the isolates were resistant to one or more of the four nitroimidazole drugs tested, namely, metronidazole, dimetridazole, secnidazole, and tinidazole. Secnidazole elicited the highest resistance rate (19.0%; 15/79), followed by tinidazole (17.7%; 14/79), metronidazole (17.7%; 14/79), and dimetridazole (13.9%; 11/79). An enormous majority of the resistant isolates (70.0%; 14/20) exhibited resistance to multiple drugs. Additionally, the resistance rate was significantly higher in isolates from birds aged < 30 days (53.3%; 8/15) than in those from older birds (23.1%; 12/52). Moreover, no drug resistance was detected in female pigeons. The genotype of the isolated strain was also associated with drug resistance. Specifically, 50.0% (15/30) of ITS-B genotypes exhibited resistance to drugs, while only 10.2% (5/49) of ITS-A genotypes demonstrated resistance. This study also found the growth characteristics of different Trichomonas isolates to be influenced by their genotypes and initial inoculum concentrations. These findings underscore the urgent need for effective measures to control and prevent drug-resistant T. gallinae infections in pigeons, thus ensuring the stable development of the pigeon industry.
The Impact of an Oceanic Mesoscale Anticyclonic Eddy in the East China Sea on the Tropical Cyclone Yagi (2018)
Authors: Sun Jianxiang , Si Jia , Cai Junhua , Chen Guangcan , Wang Kaiyue , Li Huan , Yang Dongren
Published: January 2024

In August 2018, tropical cyclone (TC) Yagi rapidly intensified as it passed over an oceanic mesoscale anticyclonic eddy (eddy) in the East China Sea, increasing in TC intensity from a tropical storm to a severe tropical storm. The maximum surface wind speed increase (minimum sea level pressure decrease) of Yagi over sea surface temperature (SST) warm anomalies caused by the eddy accounted for 66.7% (36.4%) of the whole case. Regional atmospheric model experiments are conducted to aid the interpretations of the case of Yagi intensifying over the eddy. Model results indicate that the SST warm anomalies caused by the eddy increase and concentrate the heat flux transported from the ocean to the atmosphere, and the upper air warms up, which is conducive to the development of convection. As a result, the contribution of the eddy to TC enhancement is over 20% in the period over the eddy and the precipitation is more concentrated at the TC center. The model further showed that the eddy affects the TC, resulting in more favorable dynamic and thermal conditions around the TC after landfall for the formations of TC tornadoes.