bluuebunny/crossref_metadata_2025 huggingface dataset:
Modified uvsY by N-Terminal Hexahistidine Tag Addition Enhances Efficiency of Recombinase Polymerase Amplification to Detect SARS-CoV-2 DNA.
Authors: Juma Kevin Maafu , Takita Teisuke , Yamagata Masaya , Ishitani Mika , Hayashi Kaichi , Kojima Kenji , Suzuki Koichiro , Ando Yuri , Fujiwara Shinsuke , Nakura Yukiko , Yanagihara Itaru , Yasukawa Kiyoshi
Published: November 2021

Background
Recombinase (uvsY and uvsX) from bacteriophage T4 is a key enzyme for recombinase polymerase amplification (RPA) that amplifies a target DNA sequence at a constant temperature with a single-stranded DNA-binding protein and a strand-displacing polymerase. The present study was conducted to examine the effects of the N- and C-terminal tags of uvsY on its function in RPA to detect SARS-CoV-2 DNA.
Methods
Untagged uvsY (uvsY-Δhis), N-terminal tagged uvsY (uvsY-Nhis), C-terminal tagged uvsY (uvsY-Chis), and N- and C-terminal tagged uvsY (uvsY-NChis) were expressed in Escherichia coli and purified. RPA reaction was carried out with the in vitro synthesized standard DNA at 41ºC. The amplified products were separated on agarose gels.
Results
The minimal initial copy numbers of standard DNA from which the amplified products were observed were 6 ×105, 60, 600, and 600 copies for the RPA with uvsY-Δhis, uvsY-Nhis, uvsY-Chis, and uvsY-NChis, respectively. The minimal reaction time at which the amplified products were observed were 20, 20, 30, and 20 min for the RPA with uvsY-Δhis, uvsY-Nhis, uvsY-Chis, and uvsY-NChis, respectively. The RPA with uvsY-Nhis exhibited clearer bands than that with either of other three uvsYs.
Conclusion
The reaction efficiency of RPA with uvsY-Nhis was the highest, suggesting that uvsY-Nhis is suitable for use in RPA.
Disaster Development Interface & its Impact on Emerging Vulnerability Scenario in Ladakh Region of North Western Himalayas
Authors: Bhat M Shafi , Khan Amir Ali , AKBAR MOHMAD , Sumaira Mir
Published: November 2021

The Union Territory of Ladakh located in the North-Western Himalayan Region is a multi-hazard prone region, highly vulnerable to seismic hazards, landslides, snow-avalanches, flash-floods and cloud bursts. Besides, anthropogenic hazards like border conflicts and political turmoil in neighboring region of Kashmir has resulted in huge economic loss to the region. Findings reveal that despite suffering losses by natural and human -induced disasters, the region has been positively affected by publicity generated by such events. These events managed to showcase the unique physiography, pristine environment and adventurous terrains of Ladakh, which in the subsequent years increased tourism sector as the main growth driver in the region. Furthermore, movies like LOC Kargil and 3 idiots highlighted the potential for adventurous tourism in the region ultimately attracting large number of domestic tourist to Ladakh. The exceptional growth in tourists and associated developmental sectors has resulted in enhanced levels of vulnerabilities in this environmentally fragile region on account of faulty planning practices. The study focuses on geo- physical as well as anthropogenic vulnerability analysis with the help of remote sensing and Analytical Hierarchical Processes (AHP) method. The results reveal that the region is facing an increase in climate change related vulnerabilities followed by geo- physical, socio-cultural and environmental vulnerabilities. The study highlights the need to manage emerging vulnerabilities through proper adaptation and mitigation measures by incorporation of geo physical factors in policy formulation. Proper implementation of these measures can ensure sustainable development in this fragile Himalayan region.
Inhibition of RFX6 Suppresses Invasive Ability of Tumor Cells and Activates T Cell Immune Response Though Notch Pathway in Hepatocellular Carcinoma
Authors: Song Mu , Kuerban Mulati , Zhao Lu , Peng Xiaolin , Xu Youqin
Published: November 2021

Background

DNA-binding protein RFX6 was overexpressed in hepatocellular carcinoma and its expression level was correlated with the prognosis and immune cell infiltration in liver hepatocellular carcinoma. However, the mechanism of abnormal expression and biological effects of RFX6 in liver cancer remains unknown.
Methods

To understand the specific expressing mechanism of RFX6 in liver cancer, we performed bioinformatic prediction, CHIP-qPCR assay, Co-IP and dual-luciferase assay to assess the regulating mechanism of RFX6. In the meantime, a series of biological experiments in vivo and vitro were conducted to analyze the biological significance of RFX6 in hepatocellular carcinoma.
Results

We demonstrated that the knockdown of RFX6 in liver cancer cells significantly suppressed the proliferation, migration and invasion of cancer cells. Moreover, inhibition of RFX6 could stimulate immune response of T cells. Among lots of interacting proteins, we revealed RFX6 directly binding to DTX2, regulator of Notch signaling pathway by targeting NOTCH1, and help it`s transcription stability. Furthermore, we discovered miRNA-542-3p, expression of which was decreased in hepatocellular carcinoma, was directly involved in negative regulation of the expression of RFX6.
Conclusions

In summary, we discovered a miRNA-542-3p-RFX6-DTX2-NOTCH1 regulatory pathway played significant roles in the tumor progression of liver hepatocellular carcinoma.
The Effects of Ammonia Stress Exposure on Protein Degradation, Immune Response, Degradation of Nitrogen-containing Compounds and Energy Metabolism of Chinese Mitten Crab.
Authors: Tang Dan , Wu Ya , Wu Lv , Bai Yuze , Zhou Ying , Wang Zhengfei
Published: November 2021

The Chinese mitten crab is one of the most economically important crab that is widely farmed in China. Ammonia, which is a main physiological challenge for crab culture, grow rapidly in the intensive culture system over time, but little information is available with Chinese mitten crab on the molecular mechanisms. Therefore, to understand the mechanism of response to ammonia stress in Eriocheir japonica sinensis, comparative transcriptome analysis was used to identify the key genes and pathways in hepatopancreas challenged with ammonia stress (325.07 mg/L NaCl). By sequencing the transcriptome hepatopancreas of E. j. sinensis treated with ammonia, 366,472 unigenes were obtained and annotated into several public libraries for later analyses. Subsequently, 1,775 differentially expressed genes (DEGs) were identified according to comparative transcriptome analysis, of which 307 were up-regulated and 1,468 were down-regulated. According to the DEGs of GO and KEGG enrichment analyses, we focused on four aspects of significant enrichment in this study: protein degradation, immune response, degradation of nitrogen-containing compounds and energy metabolism. The genes involved in protein degradation and energy metabolism process showed a significant decrease which was consisting of overall biological activity of E. j. sinensis decreased. In addition, five genes involved in high concentration of ammonia were discovered and validated by qRT-PCR. Therefore, this study will help us understand the molecular mechanisms of E. j. sinensis under high ammonia exposure.
A Novel Detection Performance Modular Evaluation Metric of Space-based Infrared System
Authors: Zhou Xiaoxuan , Ni Xinyue , Zhang Jingwen , Weng Dongshan , Hu Zhuoyue , Chen Fansheng
Published: November 2021

In order to reflect the space-based full chain information of the detection process comprehensively and objectively, we proposed a novel modular evaluation metric to discuss the target, background and system independently. It takes the equivalent radiation intensity as the parameter, which can evaluate the detection performance of the system quantitatively. In this paper, taking the fifth-generation American stealth fighter F22 as an example, the mathematical detection model of the space-based infrared system to aircraft targets in the Earth background is described. A modular evaluation metric is proposed. The simulation analyzes the impact of different detection scenes and system parameters on system equivalent irradiance. Furthermore, recommendations for the optimization of the detection system are given. The research results provide a new idea for the analysis of the detection performance of highly maneuverable targets under dynamic backgrounds and have guiding significance for the performance evaluation and parameter design of the infrared detection system.
Fractional Physics-informed Neural Networks for Time-fractional Phase Field Models
Authors: Wang Shupeng , Zhang Hui , Jiang Xiaoyun
Published: November 2021

In this paper, a new fractional physics-informed neural networks (fPINNs) is proposed, which combines fPINNs with spectral collocation method to solve the time-fractional phase field models. Compared to fPINNs, it has large representation capacity due to the property of spectral collocation method, which reduces the number of approximate points of discrete fractional operators, improves the training efficiency and has higher error accuracy. Unlike traditional numerical method, it directly optimizes the spectral collocation coefficient, saves the time of matrix calculation, is easy to deal with the high-dimensional model, and also has higher error accuracy. First, fPINNs based on a spectral collocation method is used to obtain the numerical solutions of the models under consideration. The spectral collocation method is used to discretize the space direction, and the fractional backward difference formula is used to approximate the time-fractional derivative. The error accuracy in different cases is discussed, and it is observed that the point-wise error is 10−5 to 10−7 . Next, fPINNs is employed to solve several inverse problems in time-fractional phase field models to identify the order of fractional derivative, mobility constant, and other coefficients. The results of numerical experiments are presented to prove the effectiveness of fPINNs in solving time-fractional phase field models and their inverse problems
Evaluation of Lamellar Macular Hole With Optical Coherence Tomography Angiography and Comparison Between Degenerative and Tractional Subtypes
Authors: Gültekin Burcu Polat
Published: November 2021

Aim
To evaluate the optical coherence tomography angiography (OCTA) findings in cases with degenerative and tractional-subtype lamellar macular hole (LMH).
Methods
Two sub-types of LMH cases were included. Thirty-seven patients had degenerative-subtype, whereas 35 patients had tractional-subtype LMH. Thirty healthy cases were enrolled as control group. Foveal avascular zone (FAZ), retinal vascular densities in superficial, deep capillary and choriocapillaris plexuses were analyzed and compared with fellow eyes and healthy controls using OCTA.
Results
Mean FAZ area was wider in the degenerative-subtype (0.33±0.14 mm²) than the tractional-subtype (0.24±0.10 mm²) (p=0.04) and control eyes (0.26±0.10 mm2) (p=0.02). Compared with the tractional group, foveal vessel density in the superficial layer was lower in the degenerative group (29.1±6.9% vs.21.9±9.2%, p=0.01). Choriocapillaris vascular density in the parafoveal area was also lower in the degenerative-subtype lamellar macular holes ( 61.8±4.7% vs. 63.2±4.2 %) (p=0.03). The vascular densities in the DCP did not disclose any significant difference between two sub-types (p>0.05). Compared with control eyes, the vascular densities in the superficial, deep and choriocapillaris layers were significantly lower in the LMH subtypes (p<0.05).
Conclusion
These OCTA changes may indicate a primary or secondary chronic degenerative process affecting the retinal microvascular plexuses in degenerative and tractional lamellar macular holes. The distinction of the pathogenesis is also demonstrated by OCTA and supports the recent change in classification and terminology of this macular pathology.
Determining ethyl methane sulfonate-mediated (EMS) mutagenesis protocol for inducing high biomass yield in fodder barley (Hordeum vulgare L.)
Authors: Sharamo Fatuma Fora , Shimelis Hussein , OlaOlorun Boluwatife ModupeOluwa , Korir Hezekiah , Indetie Annah Hoka , Mashilo Jacob
Published: November 2021

Barley (Hordeum vulgare L.) has narrow genetic base for selection of promising ideotypes for the following traits: high biomass yield for livestock feed, enhanced agronomic and nutritional traits, and resistance to biotic and abiotic stresses. Ethyl methane sulfonate (EMS) mutagenesis offers opportunities for inducing genetic variation for key traits for development of feed barley ideotypes. The objective of this study was to determine optimal EMS dosage and exposure time to induce genetic variation for selection of high biomass yield six-row feed barley mutants. Five EMS dosages (i.e. 0.1%, 0.3%, 0.5%, 0.7% and 0.9% v/v) and five exposure times (i.e. 0.5 hr, 1 hr, 1.5 hr, 2 hrs and 2.5 hrs) were used on a six-row fodder barley using a 5 x 5 factorial treatment structure in a complete randomized design with three replications. Non-treated seed were used as a comparative control. Data was recorded for percent germination, seedling survival, shoot height, root height, shoot and root biomass. Significant (p < 0.05) EMS dosage x exposure time was observed for assessed traits indicating their influence on phenotypic variation in feed barley. Overall, a declining trend was observed for assessed traits with increased EMS dosage and exposure time. The LD50 value of 0.64% (v/v) EMS dosage was identified as an optimal dose for large-scale mutagenesis protocol to select fodder barley mutants with high biomass yield
Antioxidant enzymes activity in ‘Castel Gala’ apple trees after the budbreak inducers application
Authors: Marques Léo Omar Duarte , Thiel Caroline Hernke , Leivas Gabrielle Leivas de , Deuner Sidnei , Pasa Mateus da Silveira , Herter Flavio Gilberto , Farias Paulo Mello-
Published: November 2021

Since antioxidant enzymes activity affects the end of dormancy stage, it is important to understand the responses given by antioxidant enzymes and their roles in budbreak induction in temperate fruit trees. This research aimed at evaluating the antioxidant enzymes activity at the end of budbreak stage after the inducers application to ‘Castel Gala’ apple trees and their performance in budbreak and blooming. Seven treatments were carried out: Erger® 3% + calcium nitrate 5%; Erger® 5% + calcium nitrate 5%; Erger® 7% + calcium nitrate 5%; garlic extract (GE) 5% + mineral oil (MO) 2%; GE 10% + MO 2%; GE 15% + MO 2%; and the control. Buds were collected one, four and seven days after the application (DAA) of budbreak inducers, and activity of the following enzymes was determined by: superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Besides that, hydrogen peroxide was quantified. Percentages of budbreak and blooming were evaluated at 32th, 40 th, 45 th and 52 th DAA. The results showed that SOD activity had increased as the dormancy stage reached its end. CAT activity was influenced by the application of budbreak inducers. It decreased significantly throughout the experiment in the most efficient treatment. Percentages of budbreak (61.85%) and blooming (25.44%) showed the highest efficiency in the treatment with Erger® 7% + calcium nitrate 5%, which was the inducer with the highest potential in ‘Castel Gala’ apple trees
Physico-chemical assessment of waters used to irrigate agricultural lands of Amazon
Authors: Nascimento Suziane Magalhães do , Júnior Joaquim Alves de Lima , Júnior Pedro Moreira de Sousa , Alves Mateus Higo Daves , Costa Janile do Nascimento , Faial Kelson do Carmo Freitas
Published: November 2021

The use of pesticides / fertilizers in plantations has become a problem in maintaining the quality of surface water. Thus, the objective of this study was to evaluate the water quality for irrigation purposes in the Rio Apéu microbasin – Para. The physico-chemical parameters evaluated were: pH, DO, SAR, TDS and EC measured in situ and the metals Al, Fe, Na, Ca, Mg determined in the laboratory Evandro Chagas Institute, according to the method of APHA. The average levels of pH (5.51), OD (5.04 mg L-1) are indications of normal condition in Amazonian waters. The results of Al (332 mg L-1) and Fe (5.74 mg L -1) imply the leaching of sediments from the geological formation of the region rich in these minerals, even though they present values above what is recommended by the legislation. The Richard classification allowed us to define that the waters of the study area have low salinity and sodicity, so they are not restricted to use. Thus, the results of the water quality analysis in the watershed can be concluded that it does not offer environmental problems in the use for irrigation activity
Performance of potato cultivars in an organic farming system using organic fertilizers, vermicompost and azolla
Authors: Goud Amal K. Abou El- , Masoodi Fahad R. Al- , Elzopy Karam A. , Yousry Mona M.
Published: November 2021

The challenges of food shortages and environmental pollution require the development of safe and effective alternatives to chemical fertilizers. Two field experiments were conducted during the summer season of 2019/2020 in two locations on a private farm to evaluate the performance of three potato cultivars (Sponta, Cara, and Elbieda) under different fertilizer treatments. The experiment was laid out in a split-plot design replicated thrice. The main plots had three potato cultivars, while the sub plots were comprised of seven fertilizer treatments. The results showed that the highest vegetative growth characters, viz. plant height, leaf chlorophyll index, and plant dry weight were produced by Sponta, followed by Cara, while the lowest growth attributes were recorded in Elbeida. Compared to the NPK inorganic fertilizer, organic fertilizers significantly improved the vegetative growth characteristics of potato in all treatments. Among the organic treatments, the highest growth parameters were recorded with the application of mixed organic fertilizer, vermicompost, and Azollapinnata (T1). The highest yield, viz. tuber yield, number of tubers per plant, and tuber weight, and yield quality characteristics, viz. total carbohydrates, starch, and TSS, were also recorded in T1. Our results demonstrate that the Sponta cultivar grown with the application of mixed organic fertilizer, vermicompost, and Azollapinnata (T1) produced the highest growth, yield, and quality of potato tubers. Thus, we recommend this treatment combination as a sustainable agricultural practice for potato production in similar areas
Supplying of Active Fire Protection System (APAR) in Pulo Asem Area Jakarta Timur to Increase Safety Awareness for the Community
Authors: Rini Sartika , Yulianto Hubertus Davy
Published: November 2021

Among the world record occupational safety and health (K3) accidents, the death toll in a fire is ranked just below natural disasters such as the earthquake and tsunami. Almost every type of building and almost every country in the world has experienced a history of devastating fires, whether in hotels, nightclubs, hostels, agriculture, shops or fuel depots, included a house. The question we must ask ourselves is ‘why has this tragedy occurred repeatedly and what have we learned from disasters over the past 100 years to strengthen preventive measures globally? There are three basic requirements that a fire can occur and get bigger:

The presence of fuel or flammable materials;

The existence of a lighter source; and

The presence of oxygen in the air to support combustion.

The ability to manage and reduce the risks associated with these three elements will greatly reduce the likelihood of a serious fire. So, our obligation as academics to provide knowledge and counseling about the danger of fire to the community so that they understand the danger of fire and can-do prevention and control, in addition to increasing awareness of fire hazards to create a safe and comfortable atmosphere in our environment.
Prescription profile of antiarrhythmic drugs in a group of patients in Colombia
Authors: Gaviria-Mendoza Andres , Chica-Quintero Laura Alejandra , Córtes-Avila Paula , Poveda-Hurtado Viviana , Machado-Alba Jorge Enrique
Published: November 2021

Objective: To determine the prescription patterns of antiarrhythmic drugs and variables associated with their use in a population of patients affiliated with the Colombian Health System. Methods: A cross-sectional study was performed on a population database with patients who received antiarrhythmics from March to May 2016. Sociodemographic, pharmacological and comedication variables were included. SPSS-24 was used for data analysis using X2 tests and multivariate analyses. Results: In total, 2772 patients were treated with antiarrhythmics in the evaluated period. The mean age was 70.1 ± 13.1 years, and 51.2% were women. In total, 79.4% used a β-blocker, 58.5% amiodarone and 2.9% a calcium channel blocker. Moreover, 1192 (43.0%) patients were prescribed a single antiarrhythmic, and 1580 (57.0%) received two or more. There were 2603 patients (93.9%) with comedication, including lipid-lowering drugs (62.6%), inhibitors of the renin angiotensin aldosterone system (62.6%) and antiplatelet drugs (42.0%). Age older than 65 years increased the probability of comedication (odds ratio [OR]: 2.48; 95% confidence interval [95% CI]: 1.59-3.85), and the risk was proportional to age. We identified 1364 patients treated with conditional risk medications for QT prolongation (49.2%), 68 with a possible risk (2.5%) and 171 (6.2%) with a known risk. Conclusion: Antiarrhythmic drugs recommended by clinical practice guidelines are mainly used; however, risk interactions of QT prolongation were identified and should be taken into account by physicians to avoid adverse events or complications.
Drenaje percutáneo trans-gástrico de necrosis pancreática amurallada complicado en paciente pediátrico
Authors: Priarone Carlos Maximiliano , Pinzon Fernandez Maria Virginia , Patiño-Perez Valentina , Benavidez-Ruiz Margarita María , Silva Bermudez Randol Karami
Published: November 2021

Las complicaciones de la pancreatitis aguda se dividen en sistémicas y locales, y entre estas últimas se encuentran las colecciones líquidas pancreáticas agudas, la colección necrótica aguda, el pseudoquiste y la necrosis amurallada pancreática. Se reporta el caso de niña de nueve años con pancreatitis aguda idiopática severa, quien desarrolló una necrosis pancreática amurallada de 75 x 45 mm. Por la persistencia del dolor abdominal y la intolerancia a la vía oral, a pesar del manejo médico, se indicó un abordaje percutáneo transgástrico para su tratamiento. Se obtuvo éxito técnico y terapéutico a través del drenaje de la colección, permitiendo iniciar la vía oral y disminuyendo los requerimientos de analgesia. La paciente evolucionó satisfactoriamente, con alta quirúrgica a los 13 días y retiro del catéter a los 30 días. A los cuatro meses de seguimiento se evidenció una colección recidivante asintomática, manejándose de manera conservadora. Lo anterior permite considerar que el abordaje percutáneo transgástrico es una técnica segura y eficaz; sin embargo, evaluar la efectividad y morbimortalidad población pediátrica requiere de otros estudios que posibiliten llegar conclusiones que generen mayor evidencia científica.
Written and visual expressions of authority of female monastic institutions in Medieval Livonia: 13th to 15th centuries
Authors:   Nicolaus Copernicus University, Leighton Gregory
Published: November 2021

This article presents a study of how women (specifically nuns and abbesses) were perceived in medieval Livonia. Given the significant increase in accessible academic work on the crusading movement in the eastern Baltic, scholarly considerations of the visual culture of this region, and reconsiderations of the roles played by women in the medieval world in general, this article turns from the central regions of the study of Medieval Europe to the periphery. It begins by providing a historical overview of the sources, commenting on the sparsity of specific representations of women in the narrative texts for the Livonian crusades produced in the 13th century. Following this overview, it analyses the representations of women in the vast amount of charter evidence available for the study of Livonia. The first part of the article looks at the ways these institutions were patronized as a result of their intercessory authority. Looking at donations from the 13th to the 15th century, this article also comments on the ways in which private citizens, church officials, and members of the Teutonic Order viewed these intercessory powers. The second part also considers the economic authority gained by these institutions, particularly in the form of land donations. Finally, this article addresses the ways in which women of authority styled themselves in the written documents and depicted their power in the form of visual media, particularly on seals but also in the form of architecture.
Health Information Needs of Young Chinese People Based on an Online Health Community: Topic and Statistical Analysis
Authors: Wang Jie , Wang Xin , Wang Lei , Peng Yan
Published: November 2021

Background

The internet has been widely accessible and well accepted by young people; however, there is a limited understanding of the internet usage patterns and characteristics on issues related to health problems. The contents posted on online health communities (OHCs) are valuable resources to learn about youth's health information needs.

Objective

In this study, we concurrently exploited statistical analysis and topic analysis of online health information needs to explore the distribution, impact factors, and topics of interest relevant to Chinese young people.

Methods

We collected 60,478 health-related data sets posted by young people from a well-known Chinese OHC named xywy.com. Descriptive statistical analysis and correlation analysis were applied to find the distribution and influence factors of the information needs of Chinese young people. Furthermore, a general 4-step topic mining strategy was presented for sparse short texts, which included sentence vectorization, dimension reduction, clustering, and keyword generation.

Results

In the Chinese OHC, Chinese young people had a high demand for information in the areas of gynecology and obstetrics, internal medicine, dermatology, plastic surgery, and surgery, and they focused on topics such as treatment, symptoms, causes, pathology, and diet. Females accounted for 69.67% (42,136/60,478) and young adults accounted for 87.44% (52,882/60,478) of all data. Gender, age, and disease type all had a significant effect on young people's information needs and topic preferences (P<.001).

Conclusions

We conducted comprehensive analyses to discover the online health information needs of Chinese young people. The research findings are of great practical value to carry out health education and health knowledge dissemination inside and outside of schools according to the interests of youth, enable the innovation of information services in OHCs, and improve the health literacy of young people.
An App-Based Intervention for Adolescents Exposed to Cyberbullying in Norway: Protocol for a Randomized Controlled Trial
Authors: Kaiser Sabine , Martinussen Monica , Adolfsen Frode , Breivik Kyrre , Kyrrestad Henriette
Published: November 2021

Background

Adolescents exposed to negative online events are at high risk to develop mental health problems. Little is known about what is effective for treatment in this group. NettOpp is a new mobile app for adolescents who have been exposed to cyberbullying or negative online experiences in Norway.

Objective

The aim of this paper is to provide a description of the content of the intervention and about a randomized controlled trial that will be conducted to examine the effectiveness of NettOpp. This protocol is written in accordance with the Spirit 2013 Checklist.

Methods

An effectiveness study with a follow-up examination after 3 months will be conducted to evaluate the mobile app. Adolescents will be recruited through schools and will be randomly assigned to the intervention (NettOpp) group and a waiting-list control group. The adolescents (aged 11 to 16 years) will respond to self-report questionnaires on the internet. Primary outcomes will be changes in mental health assessed with the Strengths and Difficulties Questionnaire, the WHO-Five Well-being Index, and the Child and Adolescent Trauma Screen.

Results

Recruitment will start in January 2022. The results from this study will be available in 2023.

Conclusions

There are few published evaluation studies on app-based interventions. This project and its publications will contribute new knowledge to the field.

Trial Registration

ClinicalTrials.gov NCT04176666; https://clinicaltrials.gov/ct2/show/NCT04176666

International Registered Report Identifier (IRRID)

PRR1-10.2196/31789
Patient Decision and Experience in the Multi-channel Appointment Context: An Empirical Study (Preprint)
Authors: Ye qing , Wu Hong
Published: November 2021

BACKGROUND

Long waiting time for treatment in the outpatient department has long been a complaint and influence patient experience. It is critical to schedule patients for doctors to reduce patient waiting time. Nowadays, the multi-channel appointment has been provided for patients to get medical services, especially for those with severe illnesses and remote distance.

OBJECTIVE

This study aims to explore the factors influencing patient appointment channel choice in the context of multi-channel appointments, and how channel choice affects the waiting time for offline visiting.

METHODS

We collected outpatient appointment records from both online and offline appointment channels to conduct our empirical research. The empirical analysis is conducted into two steps. We first analyze the relationship between appointment channel choice and patient waiting time, and then the relationships between three determinants and appointment channel choice. The ordinary least squares and the logistic regression model are used to obtain empirical results.

RESULTS

Our results show that a patient with an online appointment decision has a shorter consultation waiting time compared with a patient with on-site appointment (β = -0.320, p<0.001). High-quality resource demand (β = 0.349, p<0.001), high-severity disease (β = 0.011, p<0.001), and high non-disease costs (β = 0.039, p<0.001) create an obvious incentive for patients to make appointments via the Internet. Further, only the effect of non-disease cost on channel choice is lower for patients with multiple visit histories (β = -0.021, p<0.001).

CONCLUSIONS

Our study confirms the effect of Internet use on reducing patient waiting time. Patients consider both health-related risk factors and cost-related risk factors to make decisions on appointment channels. Our study produces several insights, which have implications for channel choice and patient behavior literature. More importantly, these insights as a whole, contribute to the design of appointment systems of hospitals.
Efficacy and Tolerability of Two Novel “Standard of Care” Treatments—Intranasal Esketamine Versus Intravenous Ketamine—for Treatment-Resistant Depression in Naturalistic Clinical Practice: Protocol for a Pilot Observational Study (Preprint)
Authors: Gutierrez Gilmar , Rosenblat Joshua , Hawken Emily , Swainson Jennifer , Vazquez Gustavo
Published: November 2021

BACKGROUND

Intravenous (IV) ketamine and intranasal (IN) esketamine have been studied as novel alternatives to manage treatment-resistant depression (TRD). The objective of this observational pilot study is to compare the real-world effectiveness and tolerability of IV ketamine and IN esketamine in the management of unipolar TRD.

OBJECTIVE

To compare the effectiveness (primary outcome measure) and tolerability (secondary outcome measure) of racemic ketamine and esketamine in the management of TRD in adults and provide an expert qualitative commentary on the application of IV ketamine and IN esketamine in clinical practice (exploratory objective), focusing on the recruitment process, patient retention, effectiveness, and tolerability of the treatments.

METHODS

This is a multicenter prospective observational study of naturalistic clinical practice. We expect to recruit 10 patients per treatment arm—IV ketamine or IN esketamine per center (2 centers, total 40 subjects). Patients experiencing moderate to severe TRD and who are candidates for receiving low-dose IV ketamine treatments or IN esketamine as part of their standard-of-care treatments will be recruited. We will measure the effectiveness of each treatment arm by measuring the severity of depression symptoms using the Montgomery and Åsberg Depression Rating Scale; tolerability, side effects, and the appearance of dissociation symptoms using the simplified 6-item version of the Clinician Administered Dissociative Symptom Scale (CADSS-6); and potential for abuse using a Likeability and Craving Questionnaire. Logistic regression will examine odds ratios, number needed to treat for response and remission, number needed to harm, and likelihood to be helped or harmed of each treatment. Covariate analysis will assess the impact of site and demographic variables on treatment efficacy.

RESULTS

This observational trial was approved by the Queen’s University Health Science and Affiliated Teaching Hospital’s Research Ethics Board in February 2021. The two research centers involved have started patient recruitment. Our research center (Providence Care Hospital, Kingston, Ontario) has recruited 9 patients so far. We expect to finalize data gathering by August 2022. The manuscript is expected to be published by December 2022.

CONCLUSIONS

We hypothesize that both treatments will have comparable rapid and robust antidepressant effects and similar tolerability profiles in a real-world setting for the management of TRD.

INTERNATIONAL REGISTERED REPORT

DERR1-10.2196/34711
Student Academic Performance Prediction under Various Machine Learning Classification Algorithms
Authors: Nirmala M.
Published: November 2021

Abstract: Data Mining in Educational System has increased tremendously in the past and still increasing in present era. This study focusses on the academic stand point and the performance of the student is evaluated by various parameters such as Scholastic Features, Demographic Features and Emotional Features are carried out. Various Machine learning methodologies are adopted to extract the masked knowledge from the educational data set provided, which helps in identifying the features giving more impact to the student academic performance and there by knowing the impacting features, helps us to predict deeper insights about student performance in academics. Various Machine learning workflow starting from problem definition to Model Prediction has been carried out in this study. The supervised learning methodology has been adopted and various Feature engineering methods has been adopted to make the ML model appropriate for training and evaluation. It is a prediction problem and various Classification algorithms such as Logistic Regression, Random Forest, SVM, KNN, XGBOOST, Decision Tree modelling has been done to fit the student data appropriately. Keywords: Scholastic, Demographic, Emotional, Logistic Regression, Random Forest, SVM, KNN, XGBOOST, Decision Tree.
Programa de intervenção em funções executivas pró-aprendizagem acadêmica para jovens/universitários: Desenvolvimento e evidências de validade de conteúdo
Authors: Dias Natália Martins , Costa David Mesquita da , Cardoso Caroline Oliveira , Colling Ana Paula Cervi , Fonseca Rochele Paz
Published: November 2021

Apesar de atual destaque para programas de intervenção das funções executivas (FE), predominam aqueles para crianças e idosos, com uma lacuna de programas destinados ao público jovem/universitário. O presente estudo apresenta o processo de desenvolvimento de um programa de intervenção em FE em prol da aprendizagem acadêmica para jovens/universitários. O desenvolvimento seguiu 5 etapas: 1) Interna de organização, 2) Construção, 3) Análise de Juízes, 4) Revisão e fechamento, 5) Estudo Piloto. Participaram 8 juízes (etapa 3) e 3 turmas (n = 102) ingressantes no Ensino Superior (etapa 5). Houve elevada concordância entre juízes em relação às demandas e qualidade geral das atividades. Revisão e integração das avaliações quanti e qualitativas dos juízes originou a versão atual do πFex-Academics, com sete atividades estruturadas em três módulos, com foco na aprendizagem e demandas acadêmicas. Estudo piloto revelou boa aplicabilidade. O πFex-Academics constitui-se como ferramenta promissora para estimulação de FE em contexto universitário.
PENERAPAN MODEL PEMBELAJARAN PENEMUAN TERBIMBING UNTUK MENINGKATKAN HASIL BELAJAR SISWA PADA MATERI FUNGSI INVERS DI KELAS XI IPA 2 SMA NEGERI 9 PALU
Authors: Ulfa Mariah , Sudarman Sudarman , Ismaimuza Dasa
Published: November 2021

Tujuan penelitian ini adalah untuk mendeskripsikan penerapan model pembelajaran penemuan terbimbing dalam upaya meningkatkan hasil belajar siswa pada materi fungsi invers di kelas XI IPA 2 SMA Negeri 9 Palu. Jenis penelitian ini adalah penelitian tindakan kelas (PTK). Rumusan masalah dalam penelitian ini adalah Bagaimana penerapan model pembelajaran penemuan terbimbing dapat meningkatkan hasil belajar siswa pada materi Fungsi Invers Di kelas XI IPA 2 SMA Negeri 9 Palu. Rancangan penelitian ini mengacu pada desain penelitian Kemmis dan Mc. Taggart yakni  (1) perencanaan, (2) pelaksanaan tindakan, (3) observasi dan (4) refleksi. Penelitian ini dilakukan dalam dua siklus. Hasil penelitian menunjukkan bahwa penerapan model penemuan terbimbing dapat meningkatkan hasil belajar siswa pada materi fungsi invers di kelas XI IPA 2 SMA Negeri 9 Palu mengikuti langkah-langkah Penemuan terbimbing  yaitu (1) perumusan masalah,(2) pemprosesan data dan penyusunan konjektur, (3) pemeriksaan dan verbalisasi konjektur, dan (4)  umpan balik. Jenis data yang digunakan adalah data kualitatif dan data kuantitatif dengan teknik pengumpulan data yaitu tes, observasi, catatan lapangan dan wawancara. Penelitian ini dilakukan dalam dua siklus. Hasil penelitian ini menunjukkan bahwa terjadi peningkatan hasil belajar siswa yang terlihat dari ketuntasan belajar klasikal siswa yang mengalami peningkatan dari siklus I ke siklus II, lembar hasil observasi aktivitas siswa dan guru kategori baik pada siklus I dan lembar hasil observasi aktivitas siswa kategori baik, lembar hasil observasi aktivitas guru kategori sangat baik pada siklus II. Berdasarkan hasil tersebut, dapat disimpulkan bahwa penerapan model pembelajaran penemuan terbimbing dapat meningkatkan hasil belajar siswa pada materi fungsi invers di kelas XI IPA2 SMA Negeri 9 Palu.
Physical-Chemical Properties of Compressible Clathrates: A Natural Pressure Shift by Extending the van der Waals and Platteeuw Model
Authors: Segtovich Iuri Soter Viana , Medeiros Fernando de Azevedo , Tavares Frederico Wanderley
Published: November 2021

We have developed a new model for compressible clathrates that extends
the well-known van der Waals and Platteeuw model. The new model is
derived by dispensing with the assumption of constant cages radii in the
partition function level, resulting in new thermodynamically consistent
expressions relating thermodynamic properties of the hydrate phase and
the empty lattice isochoric reference. One set of additional parameters
to the clathrate modeling framework is introduced, consisting of a
scaling factor for each cage radius relative to the edge length of the
unit cell. No additional guest-dependent empirical parameters are
required. The model exhibits two features not previously reported in the
literature: (i) a pressure shift between the clathrate being described
and the empty lattice isochoric reference, and (ii) differences in the
edge length of the unit cell and in the cages radii for different guest
species at the same temperature and pressure, as a consequence of the
sorption of guests. We also propose a test for thermodynamic consistency
at high pressure, based on the multicomponent and multiphase Clapeyron
equation. Using this test, we show that the proposed model solves an
inconsistency issue observed in phase equilibrium calculations with some
of the compressible clathrate models currently in use. We have performed
parameter optimization for methane, ethane, and xenon in sI hydrates.
Two sets of results are presented: 3-phase equilibrium conditions; and
lattice size versus temperature or pressure for each of these
substances, along with available experimental data.
Regulatory Treatment of Changes in Fair Value and the Composition of Banks' Investment Portfolios
Authors: Iselin Michael , Kang Jung Koo , Madsen Joshua
Published: November 2021

Following the financial crisis of 2007–2008, Basel III recommended that bank regulators include changes in the fair value of available-for-sale (AFS) debt securities in Tier 1 capital. However, the U.S. allowed smaller banks to continue excluding these changes through a one-time opt out election. This paper examines the investment decisions of smaller banks in the 1990s when changes in the fair value of AFS debt securities were temporarily included in regulatory capital. Using a sample of smaller banks and a difference-in-differences research design, we find that low-capitalized banks reduced their investments in more volatile asset classes (e.g., corporate bonds, non-agency MBS) and increased their investments in less volatile asset classes (e.g., treasuries and municipal bonds) after these changes were included. These findings suggest that providing smaller banks with an opt out election potentially allows low-capitalized, riskier banks to continue to hold more volatile securities in their AFS portfolios.

JEL Classifications: M41; M48; G18; G21.
PENGARUH TINGKAT PENDIDIKAN, PENGANGGURAN , DAN KEMISKIAN TERHADAP KRIMINALITAS DI INDONESIA
Authors: Rahmalia Suci , Ariusni Ariusni , Triani Mike
Published: November 2021

This study aims to determine and analyze the influence of (1) Level of Education, (2) Unemployment, and (3) Poverty against crime in Indonesia by using the panel regression equation model and using the Fixed Effect Model (FEM) approach. The estimation results show that (1) the level of education has a negative and not significant effect on criminality in Indonesia, (2) unemployment has a negative and significant effect on crime in Indonesia, (3) poverty has a positive and significant influence on crime in Indonesia.This type of research is descriptive and associative. Data type is secondary data. This study uses panel data, which uses 31 provinces in Indonesia using the Fixed Effect Model (FEM) approach.The results of this study indicate that: (1) The level of education has a negative and insignificant influence on crime in Indonesia, (2) Unemployment has a negative and significant effect on crime in Indonesia, (3) Poverty has a positive and significant influence on crime in Indonesia.
PENGARUH INVESTASI ASING (FDI), KONSUMSI RUMAH TANGGA, PENGELUARAN PEMERINTAH TERHADAP PEREKONOMIAN INDONESIA
Authors: Varlina Iin , Amar Syamsul
Published: November 2021

This study aims to find out how the influence of Foreign Direct Investment (FDI), household concumption, government expenditure of the Indonesian economy. The data used are secondary data in the form of time series from 1983 to 2017, with documentation data collection techniques and library studies obtained from relevant institutions and agencies. The variables used are Indonesian Economy (Y), Foreign Direct Investment (FDI) (X1), household consumption (X2), government expenditure (X3). The research methods used are: (1) Ordinary Last Square (OLS), (2) Classical Assumption Test, (3) T Test and (4) F Test. The results of the study show that (1) Foreign Direct Investment has a positive and significant effect on the economy Indonesia. (2) household consumption has a positive and significant effect on the economy Indonesia. (3) Government Expenditiure has a positive and significant effect on the economy Indonesia.
ANALISIS MEKANISME TRANSMISI KEBIJAKAN MONETER TERHADAP INFLASI
Authors: Sari Mita Ratna , Yeniwati Yeniwati
Published: November 2021

This study aims to determine the effect of the BI Rate through the transmission of interest rates on Bank Indonesia Certificates (SBI), interest rates on the Interbank Money Market (PUAB), loan interest rates and deposit rates on inflation. The type of research used is associative descriptive research. The type of data in this study is secondary data and time series in the form of quarterly data from 2001 to 2016. Analysis of the data used is descriptive analysis and inductive analysis. The method used is Moderated Regression Analysis (MRA), in the inductive analysis there are tests, namely: Moderated Regression Analysis (MRA), classic assumption test and t test. These results show that: (1) the BI Rate has a significant effect through the transmission of SBI interest rates to inflation (2) the BI Rate has a significant effect through the transmission of interbank rates to inflation (3) the BI Rate has a significant effect through transmission of lending rates to inflation (4) The BI Rate has no significant effect through the transmission of deposit rates to inflation.
ANALISIS KAUSALITAS INVESTASI ASING LANGSUNG (FDI), EKSPOR DAN PERTUMBUHAN EKONOMI DI INDONESIA
Authors: Ikhwan Radha , Ariusni Ariusni
Published: November 2021

This study examines the causal relationship between foreign direct investment (FDI), exports and economic growth in Indonesia by using Vector Error Corroction Estimates (VECM) time series models during the period 1982 - 2017. The results show that (1)There is no causal relationship between economic growth and investment foreign direct investment (FDI), there is a one-way relationship between foreign direct investment (FDI) and economic growth, where only foreign direct investment (FDI) affects economic growth. (2)There is no causal relationship between economic growth and exports, where there is no influence between the two. (3)There is no causal relationship between foreign direct investment (FDI) and exports, where there is a one-way relationship between foreign direct investment (FDI) and exports, where only foreign direct investment (FDI) affects exports.
ANALISIS KAUSALITAS TEKNOLOGI INFORMASI KOMUNIKASI (TIK) DAN PERTUMBUHAN EKONOMI DI ASEAN
Authors: Sahrina Sahrina , Anis Ali
Published: November 2021

This study examined the causal relationship between fixed telephone subscriptions, mobile cellular subscriptions, fixed broadband subscriptions, internet users and economic growth using a panel vector autoregrassion model (PVAR) for ASEAN’s five founding countries in the period of 2001-2015. The results of this study showed that are there is no causality between Fixed Telephone subcription and economic growth, an  unidirectional relationship between mobile celluler subscriptions and economic growth where is that just economic growth gives influence to mobile cellular subscriptions, an unidirectional relationship between fixed broadband subscription and economic growth where is that just fixed broadband subscriptions gives influence to economic growth and an unidirectional relationship from economic growth to internet users where is that just economic growth gives influence to mobile cellular subscriptions.