bluuebunny/crossref_metadata_2025 huggingface dataset:
Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model
Authors: Tazawa Ryo , Uchida Kentaro , Minehara Hiroaki , Matsuura Terumasa , Kawamura Tadashi , Sekiguchi Hiroyuki , Muneshige Kyoko , Inoue Sho , Inoue Gen , Takaso Masashi
Published: October 2020

Background

Delivery of bone morphogenetic protein-2 (BMP-2) via animal-derived absorbable collagen materials is used for the treatment of large bone defects. However, the administration of bovine proteins to humans is associated with the risk of zoonotic complications. We therefore examined the effect of combining BMP-2 with collagen-like peptides, poly(POG)n, in a critical-sized bone defect mouse model.

Methods

A 2-mm critical-sized bone defect was created in the femur of 9-week-old male C57/BL6J mice. Mice were randomly allocated into one of four treatment groups (n = 6 each): control (no treatment), poly(POG)n only, 0.2 μg, or 2.0 μg BMP-2 with poly(POG)n. New bone formation was monitored using soft X-ray radiographs, and bone formation at the bone defect site was examined using micro-computed tomography and histological examination at 4 weeks after surgery.

Results

Administration of 2.0 μg of BMP-2 with poly(POG)n promoted new bone formation and resulted in greater bone volume and bone mineral content than that observed in the control group and successfully achieved consolidation. In contrast, bone formation in all other groups was scarce.

Conclusions

Our findings suggest the potential of BMP-2 with poly(POG)n as a material, free from animal-derived collagen, for the treatment of large bone defects.
Flexible versus standard intramedullary rod in posterior stabilized primary total knee arthroplasty: protocol for a randomized controlled trial
Authors: Bénard M. R. , van Doremalen R. F. M. , Wymenga A. B. , Heesterbeek P. J. C.
Published: October 2020

Background

In total knee arthroplasty (TKA) a flexible intramedullary rod can be used to account for sagittal bowing of the distal femur. Although patients report better post-operative functional outcome when the flexible rod was used, it is unknown how the use of the flexible rod affects the placement of the femoral TKA component, and how this relates to activities of daily living. It is expected that the use of the flexible rod will result in a more flexed femoral component, a larger patellar tendon moment arm, and consequently in better functional outcome. The goal of this study is to compare the flexible rod to the standard intramedullary rod in primary TKA in terms of fit of the TKA, functional outcome, and sizing of the femoral component.

Methods

A single-blind randomized controlled trial with two groups (flexible vs standard rod), with patients blinded for group allocation, and 2 years post-operative follow-up. The fit of the TKA is quantified by two parameters: (1) the flexion angle of the TKA in the sagittal plane and (2) the sagittal profile of the distal femur compared between the pre-operative bone and the TKA. Both parameters are calculated in 3D volume images obtained using fluoroscopy. Functional outcome will be measured using (1) the timed Get-up and Go test (2), the stair climbing test (3), knee power output, and (4) patient and clinician reported outcomes. Different parameters will be measured during the TKA procedure to account for the invisibility of cartilage in the 3D volumes and to study if the amount of bone removed during the procedure is affected by group allocation.

Discussion

The sagittal fit of TKA is not a standardized outcome measure. We discuss our choice of parameters to define the sagittal fit (i.e., flexion angle and sagittal profile), our choice for the parameters we measure during the TKA procedure to account for the lack of cartilage thickness in fluoroscopy, and our choice for the parameters to study if the amount of bone removed during the procedure is affected by group allocation. Lastly, we discuss the merits of this planned trial.

Trial registration

Netherlands Trial Register,4888, registered 30 March 2015.https://www.trialregister.nl/trial/4888
One-stage posterior debridement and single-segment interbody fusion for treating mono-segmental lumbar and lumbosacral spinal tuberculosis in adults following minimum 5-year follow-up
Authors: Xu Zhenchao , Wang Xiyang , Liu Zheng
Published: October 2020

Background

To evaluate the mid-long-term outcomes of surgical management of mono-segmental lumbar and lumbosacral spinal tuberculosis (TB) in adults by one-stage posterior debridement, single-segment fixation, and titanium mesh cage interbody fusion.

Methods

A total of 62 patients with mono-segmental lumbar or lumbosacral spinal tuberculosis were enrolled. One-stage posterior debridement, single-segment fixation, and titanium mesh cage interbody fusion was performed. Clinical and radiographic outcomes were compared and analyzed.

Results

All patients were followed-up for an average of 75.0 ± 11.5 months and completely cured at the final follow-up. C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) returned to normal within three months postoperatively. Postoperative Japanese Orthopedic Association (JOA) score, visual analog scale (VAS) and Oswestry Disability index (ODI) were significantly improved compared with preoperative values. Bony fusion occurred after an average of 9.8 ± 2.6 months. The lordosis angle and lumbosacral angle were increased from preoperative 20.4 ± 2.9° and 14.7 ± 3.4° to postoperative 32.8 ± 3.6° and 22.4 ± 5.5°, with angle loss of 1.0 ± 0.7° and 0.8 ± 0.7° at the final follow-up, respectively. No significant differences between preoperative and postoperative adjacent segment disc height (DH) were found.

Conclusions

One-stage posterior debridement, single-segment fixation, and titanium mesh cage interbody fusion represent effective and feasible treatment option for mono-segmental lumbar and lumbosacral spinal tuberculosis in adults. This approach may preserve lumbar normal motor units and decrease adjacent segment degeneration (ASD) with the advantages of minimal invasiveness and rapid postoperative rehabilitation.
Diagnosis of hepatic glycogen storage disease patients with overlapping clinical symptoms by massively parallel sequencing: a systematic review of literature
Authors: Beyzaei Zahra , Geramizadeh Bita , Karimzadeh Sara
Published: October 2020

Background

Glycogen storage diseases (GSDs) with liver involvement are complex disorders with similar manifestations. Currently, the main diagnostic methods such as tissue diagnosis, either histopathology or enzyme assay, are invasive. Meanwhile, GSDs are diseases with significant genetic heterogeneity, and gene-sequencing methods can be more useful. This systematic review aims to review the literature to assess the value of massively parallel sequencing in the diagnosis of GSDs on patients with previously undiagnosed hepatic involvement.

Methods

Relevant studies identified in the MEDLINE/PubMed, EMBASE, Cochrane Library, Scopus, and Web of Science Core Collection databases up to July 2019 with no time and language restrictions. Publications were included in the review if they analyzed GSDs with hepatic involvement (GSD I, GSD III, GSD IV, GSD VI, GSD IX), using targeted gene sequencing (TGS) or exome sequencing (ES).

Results

Eleven studies were included in this systematic review. ES demonstrated a 93% diagnostic yield. These methods correctly distinguished all types of pathogenic variants. The diagnostic yield of the TGS method was around 79.7%.

Conclusions

According to our results, TGS analysis can be considered as the first-line diagnostic method with valuable results and ES can be used to diagnose complex cases of GSD with liver involvement. Overall, these molecular methods are considered as accurate diagnostic tools, which expedite correct diagnosis and treatment with significant cost-effectiveness by reducing unnecessary and inaccurate tests.

PROSPERO registration

CRD42020139931. Registered 8 January 2020.
Patient-reported outcomes on familial amyloid polyneuropathy (FAP)
Authors: Bolte Fabian J. , Langenstroer Christel , Friebel Frauke , Hüsing-Kabar Anna , Dugas Martin , Schmidt Hartmut H.
Published: October 2020

Background

Transthyretin familial amyloid polyneuropathy (ATTR-FAP) is a rare autosomal dominant inherited disease affecting multiple organ systems. ATTR-FAP patients’ experiences have rarely been documented. The aim of this study was to collect patient reported outcomes across different countries to assess unmet needs and challenges. An anonymous survey was conducted at the 2nd European meeting on ATTR amyloidosis in Berlin in September 2019. Survey questions captured information on demographics, clinical characteristics, diagnostic experience, quality of life, disability and ATTR-FAP management.

Results

A total of 38 ATTR-FAP patients from 15 different countries participated in the survey. ATTR-FAP had a substantial impact on patients’ day-to-day life, including difficulties in standing, walking, and participation in community activities. It also had negative effects on the mental health of patients. The survey highlighted several unmet needs and challenges from a patients’ perspective, including (i) a need for increased awareness and a standardized diagnostic pathway, (ii) a need for better treatment access and supportive care and (iii) a need for better information about research and clinical trials.

Conclusions

This global patient survey provides valuable findings to address ATTR-FAP patients’ needs and challenges in order to further the goal of patient-centered care.
Effectiveness of a home-based telerehabilitation system in patients after total hip arthroplasty: study protocol of a randomized controlled trial
Authors: Busso Chiara , Castorina Gabriele , Di Monaco Marco , Rodriguez Daniel , Mahdavi Hadis , Balocco Simone , Trucco Marco , Conti Marco , Castagna Alessandro , Minetto Marco Alessandro
Published: October 2020

Background

The demand for total hip arthroplasty (THA) is quickly rising given the escalating global incidence of hip osteoarthritis, and it is widely accepted that the post-surgery rehabilitation is key to optimize outcomes. The overall objective of this study is to evaluate the effectiveness of a new telerehabilitation solution, ReHub, for the physical function and clinical outcome improvement following THA. The specific aims of this manuscript are to describe the study design, protocol, content of interventions, and primary and secondary outcomes and to discuss the clinical rehabilitation impact of the expected experimental results.

Methods/design

This prospective, randomized, controlled, parallel-group trial will include 56 patients who had undergone primary THA. Patients are randomized to a control group (standard rehabilitation during the 2-week stay in the rehabilitation clinic followed by 3 weeks of unsupervised home-based rehabilitation) or an experimental group (standard rehabilitation during the 2-week stay in the rehabilitation clinic followed by 3 weeks of home-based ReHub-assisted telerehabilitation). The primary outcome is physical performance assessed through the Timed Up-and-Go (TUG) test. Secondary outcomes include independence level, pain intensity, hip disability, hip range of motion, muscle strength, and patient’s perception of clinical improvement.

Discussion

Proving the clinical and cost-effectiveness of a home-based telerehabilitation program for physical and muscle function following THA could support its systematic incorporation in post-surgical rehabilitation protocols, which should be tailored to the individual and collective needs.

Trial registration

ClinicalTrial.gov NCT04176315. Registered on 22 November 2019
Transcription factor FTZ-F1 regulates mosquito cuticular protein CPLCG5 conferring resistance to pyrethroids in Culex pipiens pallens
Authors: Xu Yang , Yang Xiaoshan , Sun Xiaohong , Li Xixi , Liu Zhihan , Yin Qi , Ma Lei , Zhou Dan , Sun Yan , Shen Bo , Zhu Changliang
Published: October 2020

Background

Culex pipiens pallens poses a serious threat to human health because of its widespread distribution, high carrier capacity for several arboviruses, frequent human-biting, and growth in urban environments. Pyrethroid insecticides have been mainly used to control adult Cx. pipiens pallens during outbreaks of mosquito-borne diseases. Unfortunately, mosquitoes have developed resistance, rendering the insecticides ineffective. Cuticular resistance is the primary mechanism of pyrethroid resistance. Previously, we revealed that cuticular protein of low complexity CPLCG5 is a major cuticular protein associated with deltamethrin resistance in Cx. pipiens pallens, which is enriched in the cuticle of mosquitoes’ legs and participates in pyrethroid resistance by forming a rigid matrix. However, the regulatory mechanisms of its transcription remain unknown.

Results

First, qRT-PCR analysis revealed that the expression of FTZ-F1 (encoding Fushi tarazu-Factor 1) was ~ 1.8-fold higher in the deltamethrin-resistant (DR) than deltamethrin-susceptible (DS) strains at 24 h post-eclosion (PE) and ~ 2.2-fold higher in the DR strain than in the DS strain at 48 h PE. CPLCG5 and FTZ-F1 were co-expressed in the legs, indicating that they might play an essential role in the legs. Dual luciferase reporter assays and EMSA (electrophoretic mobility shift experiments) revealed that FTZ-F1 regulates the transcription of CPLCG5 by binding to the FTZ-F1 response element (− 870/− 864). Lastly, knockdown of FTZ-F1 not only affected CPLCG5 expression but also altered the cuticle thickness and structure of the legs, increasing the susceptibility of the mosquitoes to deltamethrin in vivo.

Conclusions

The results revealed that FTZ-F1 regulates the expression of CPLCG5 by binding to the CPLCG5 promoter region, altering cuticle thickness and structure, and increasing the susceptibility of mosquitoes to deltamethrin in vivo. This study revealed part of the mechanism of cuticular resistance, providing a deeper understanding of insecticide resistance.
Awareness of cognitive decline trajectories in asymptomatic individuals at risk for AD
Authors: Cacciamani Federica , Sambati Luisa , Houot Marion , Habert Marie-Odile , Dubois Bruno , Epelbaum Stéphane ,   on behalf of the INSIGHT-PreAD study group, Audrain C. , Auffret A. , Bakardjian H. , Baldacci F. , Batrancourt B. , Benakki I. , Benali H. , Bertin H. , Bertrand A. , Boukadida L. , Cacciamani F. , Causse V. , Cavedo E. , Cherif Touil S. , Chiesa P. A. , Colliot O. , Dalla Barba G. , Depaulis M. , Dos Santos A. , Dubois B. , Dubois M. , Epelbaum S. , Fontaine B. , Francisque H. , Gagliardi G. , Genin A. , Genthon R. , Glasman P. , Gombert F. , Habert M. O. , Hampel H. , Hewa H. , Houot M. , Jungalee N. , Kas A. , Kilani M. , La Corte V. , Le Roy F. , Lehericy S. , Letondor C. , Levy M. , Lista S. , Lowrey M. , Ly J. , Makiese O. , Masetti I. , Mendes A. , Metzinger C. , Michon A. , Mochel F. , Nait Arab R. , Nyasse F. , Perrin C. , Poirier F. , Poisson C. , Potier M. C. , Ratovohery S. , Revillon M. , Rojkova K. , Santos-Andrade K. , Schindler R. , Servera M. C. , Seux L. , Simon V. , Skovronsky D. , Thiebaut M. , Uspenskaya O. , Vlaincu M.
Published: October 2020

Background

Lack of awareness of cognitive decline (ACD) is common in late-stage Alzheimer’s disease (AD). Recent studies showed that ACD can also be reduced in the early stages.

Methods

We described different trends of evolution of ACD over 3 years in a cohort of memory-complainers and their association to amyloid burden and brain metabolism. We studied the impact of ACD at baseline on cognitive scores’ evolution and the association between longitudinal changes in ACD and in cognitive score.

Results

76.8% of subjects constantly had an accurate ACD (reference class). 18.95% showed a steadily heightened ACD and were comparable to those with accurate ACD in terms of demographic characteristics and AD biomarkers. 4.25% constantly showed low ACD, had significantly higher amyloid burden than the reference class, and were mostly men. We found no overall effect of baseline ACD on cognitive scores’ evolution and no association between longitudinal changes in ACD and in cognitive scores.

Conclusions

ACD begins to decrease during the preclinical phase in a group of individuals, who are of great interest and need to be further characterized.

Trial registration

The present study was conducted as part of the INSIGHT-PreAD study. The identification number of INSIGHT-PreAD study (ID-RCB) is 2012-A01731-42.
Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP
Authors: Hien Annie , Molinaro Gemma , Liu Botao , Huber Kimberly M. , Richter Joel D.
Published: October 2020

Background

Mutations in TSC2 are the most common cause of tuberous sclerosis (TSC), a disorder with a high incidence of autism and intellectual disability. TSC2 regulates mRNA translation required for group 1 metabotropic glutamate receptor-dependent synaptic long-term depression (mGluR-LTD) and behavior, but the identity of mRNAs responsive to mGluR-LTD signaling is largely unknown.

Methods

We utilized Tsc2+/− mice as a mouse model of TSC and prepared hippocampal slices from these animals. We induced mGluR-LTD synaptic plasticity in slices and processed the samples for RNA-seq and ribosome profiling to identify differentially expressed genes in Tsc2+/− and following mGluR-LTD synaptic plasticity.

Results

Ribosome profiling reveals that in Tsc2+/− mouse hippocampal slices, the expression of several mRNAs was dysregulated: terminal oligopyrimidine (TOP)-containing mRNAs decreased, while FMRP-binding targets increased. Remarkably, we observed the opposite changes of FMRP binding targets in Fmr1−/y hippocampi. In wild-type hippocampus, induction of mGluR-LTD caused rapid changes in the steady-state levels of hundreds of mRNAs, many of which are FMRP targets. Moreover, mGluR-LTD failed to promote phosphorylation of eukaryotic elongation factor 2 (eEF2) in TSC mice, and chemically mimicking phospho-eEF2 with low cycloheximide enhances mGluR-LTD in TSC mice.

Conclusion

These results suggest a molecular basis for bidirectional regulation of synaptic plasticity and behavior by TSC2 and FMRP. Our study also suggests that altered mGluR-regulated translation elongation contributes to impaired synaptic plasticity in Tsc2+/− mice.
Venous thromboembolism and COVID-19: a case report and review of the literature
Authors: Bhatt Harshil , Singh Sandeep
Published: October 2020

Background

Currently, there is minimal data available highlighting the prevalence of venous thromboembolism in patients infected with coronavirus disease 2019 (COVID-19). This case report with a literature review emphasizes a unique presentation of COVID-19 that is highly important for health care providers to consider when treating their patients.

Case report

A 65-year-old Caucasian male patient presented to the emergency department with a 2-day history of dyspnea on exertion after his wife’s recent diagnosis of COVID-19. He additionally had experienced a couple of episodes of self-resolving diarrhea a few days before presentation. Based on the patient’s clinical presentation and the laboratory workup identifying an elevated D-dimer, a computed tomography angiogram of the chest was obtained, which was significant for moderately large, bilateral pulmonary emboli with a saddle embolus, and an associated small, left lower lobe, pulmonary infarct. Ultrasound of the lower extremity showed non-occlusive deep vein thrombosis at the distal left femoral vein to the left popliteal vein. The patient was additionally diagnosed with COVID-19 when the results of the COVID-19 polymerase chain reaction test returned as positive. The patient was admitted to the COVID unit, and he was started on an intravenously administered, unfractionated heparin drip for management of his bilateral pulmonary emboli and deep vein thrombosis. The patient’s clinical condition improved significantly with anticoagulation, and he was observed in the hospital for 3 days, after which he was discharged home on the enoxaparin bridge with warfarin. Post-discharge telephone calls at day 10 and week 4 revealed that the patient was appropriately responding to anticoagulation treatment and had no recurrence of his symptoms related to venous thromboembolism and COVID-19.

Conclusion

As COVID-19 continues to lead to significant mortality, more data is emerging that is exposing its perplexing pathogenicity. Meanwhile, the presentation of venous thromboembolism in patients with COVID-19 remains an unusual finding. It is imperative for health care providers to be mindful of this unique association to make necessary diagnostic evaluations and provide appropriate treatment for the patients.
Research on PDMA communication system based on complete complementary sequence
Authors: Li Shufeng , Su Baoxin , Jin Libiao
Published: October 2020

Pattern division multiple access (PDMA) is a new non-orthogonal multiple access (NOMA) technology. It is proposed to meet the challenge of 5G large-scale connectivity and high-frequency spectral efficiency. Compared with traditional orthogonal multiple access (OMA), PDMA can support more users through the allocation of non-orthogonal resources. Due to perfect aperiodic correlation, complete complementary sequence (CCS) greatly improves the spectrum efficiency of the system. It has been widely used in wireless communication and radar sensing, and it still has research value in 5G. In order to apply the advantages of CCS to NOMA communication system, this paper proposes a system model of CCS spread spectrum coding based on PDMA. CCS is used as spread spectrum code to improve the performance of PDMA communication system. At the same time, on the basis of spread spectrum technology, this paper analyzes the average power allocation algorithm and water-filling power allocation algorithm, and a dynamic power allocation algorithm based on the transmission rate and practical application is proposed. The simulation results show that the system model can effectively improve the performance of the system.
Computational intelligence-based connectivity restoration in wireless sensor and actor networks
Authors: Mohammadi Solmaz , Farahani Gholamreza
Published: October 2020

Network failure is categorized into the two types of software and hardware (physical layer) failure. This paper focuses on the physical layer failure in the wireless sensor and actor networks (WSANs). Actors play an important role in data processing, decision-making, and performing appropriate reactions. Single or multiple nodes failure of actors due to the explosion, energy depletion, or harsh environments, can cause multiple disjoint partitions. This paper has proposed a new computational intelligence-based connectivity restoration (CICR) method. It uses a combination of advanced computational intelligence methods to solve restoration problem. The proposed algorithm applies the novel enhanced Lagrangian relaxation with a novel metaheuristic sequential improved grey wolf optimizer (SIGWO) search space algorithm in simultaneous selection ofksponsor andppathway nodes. The reactive proposed method aims to reduce the travel distance or moving cost and communication cost. As a result, the restored network has minimum of topology change and energy consumption. In terms of total traveled distance, CICR has 37.19%, 71.47%, and 44.71% improvement in the single-node failure averagely in comparison with HCR, HCARE, and CMH, respectively. Also, it has an average of 61.54%, 40.1%, and 57.76% improvement in comparison with DCR, PRACAR, and RTN in multiple partitions resulted from multiple nodes failure, respectively. The reliability of CICR method has improved averagely by 35.85%, 38.46%, 22.03% over HCR, CMH, and HCARE in single-node failure. In multiple nodes failure, reliability of CICR has averagely 61.54% and 20% over DCR and PRACAR, respectively.
Coderivatives and Aubin property of efficient point and efficient solution set-valued maps in parametric vector optimization
Authors: Xue Xiaowei
Published: October 2020

The aim of this paper is computing the coderivatives of efficient point and efficient solution set-valued maps in a parametric vector optimization problem. By using a method different from the existing literature we establish an upper estimate and explicit expression for the coderivatives of an efficient point set-valued map where the independent variable can take values in the whole space. As an application, we give some characterizations on the Aubin property of an efficient point map and an explicit expression of the coderivative for an efficient solution map. We provide several examples illustrating the main results.
A new q-integral identity and estimation of its bounds involving generalized exponentially μ-preinvex functions
Authors: Awan Muhammad Uzair , Talib Sadia , Kashuri Artion , Noor Muhammad Aslam , Noor Khalida Inayat , Chu Yu-Ming
Published: October 2020

In the article, we introduce the generalized exponentially μ-preinvex function, derive a new q-integral identity for second order q-differentiable function, and establish several new q-trapezoidal type integral inequalities for the function whose absolute value of second q-derivative is exponentially μ-preinvex.
The need for financial knowledge acquisition tools and technology by small business entrepreneurs
Authors: Rachapaettayakom Panita , Wiriyapinit Mongkolchai , Cooharojananone Nagul , Tanthanongsakkun Suparatana , Charoenruk Nuttirudee
Published: October 2020

Knowledge management tools and technology are the main factors bringing competitive advantage to organisations. This study extends the depth of study on small business entrepreneurs by examining the effect of knowledge acquisition, in particular financial knowledge that leads to the need for knowledge acquisition tools and technology. There is evidence to support that a lower level of existing financial knowledge in small business entrepreneurs encourages them to acquire more financial knowledge since they tend to be responsible for all tasks related to business survival. This finding is not consistent with several other previous studies. In addition, relevant knowledge on how to raise adequate capital to establish their business and the accurate calculation of cost are the two most important aspects of financial knowledge for encouraging small business entrepreneurs to seek effective knowledge acquisition tools and technology. The evidence provides insight for the government authorities supporting small businesses to provide financial knowledge to small business entrepreneurs during the pre-start-up phase and thereafter by providing the relevant knowledge on financing and cost calculation to strengthen and sustain these businesses. This will ultimately lead to an improvement in small business failure and increase the country’s economic growth.
Risk Assessment of Aged Concrete Gravity Dam Subjected to Material Deterioration Under Seismic Excitation
Authors: Nahar Tahmina Tasnim , Cao Anh-Tuan , Kim Dookie
Published: October 2020

This paper proposes an approach to assess and predict the seismic risk of existing concrete gravity dams (CGDs) considering the ageing effect. The combination of fragility function and cumulative absolute velocity (CAV) depending on two failure states has been used in the analysis. It represents the time-variant degradation of the concrete structure and the conditional change of structural vulnerability in the case of the seismic excitation. Therefore, the seismic risk assessment captures here the nonlinear dynamic behavior of a concrete gravity dam through the fragility analysis. Incremental dynamic analysis for the fragility curves is adopted to state the performance of the dam in terms of different intensity measures. To assess the capacity of the aged concrete gravity dam, this research introduces a way to estimate the CAVlimit of CGDs with varying time. For a case study, an existing concrete gravity dam in Korea has been taken into consideration to apply this approach. The numerical finite element model is validated by optimizing the recorded field data. The proposed approach and its findings will be helpful to CGDs operators to ensure whether a dam needs to stop after a specific time using the extracted mathematical model. Furthermore, as this mathematical model is the function of time, the operator can get an idea about dam conditions at any specific time and can take necessary steps.
The effect of life coaching on psychological distress among dental students: interventional study
Authors: Aboalshamat Khalid , Al-Zaidi Duha , Jawa Duha , Al-Harbi Hanouf , Alharbi Raghad , Al-Otaibi Shahad
Published: October 2020

Background

Depression, stress, and anxiety are common psychological conditions among dental students in many countries around the world. A number of researchers have found life coaching to be effective at reducing psychological distress. The aim of this study was to assess the effect of a life coaching program on dental students’ psychological status.

Methods

A quasi-experiment study with two arms was conducted on 88 female dental students at Umm Al-Qura University (study group = 44; control group = 44). The psychological status was assessed by questionnaire before and after intervention. The questionnaire was composed of the Depression and Anxiety Stress Scale (DASS-21), Resilience Scale (RS-14), the Psychological Well-Being Scale–Short (PWB-S), and goal approach questions. The study group received a coaching program comprising one lecture for 1 h and five phone coaching sessions over 5 weeks, while the control group received no intervention.

Results

The study group showed a significant reduction in depression, anxiety, stress, resilience, and self-acceptance according to the PWB-S scale. Also, goal approach was significantly improved. On the other hand, the control group showed a significant reduction on the RS-14 only. The differences in the tested scales between the study group and the control group from pre-intervention (T1) to post-intervention (T2) showed significant differences in depression, stress, self-acceptance, and goal approach measurements per t-test.

Conclusion

The study’s findings showed that life coaching had the effect of reducing psychological distress, which encouraged the implementation of coaching practice in the daily life of dental students.
Oxygen administration for postoperative surgical patients: a narrative review
Authors: Suzuki Satoshi
Published: October 2020

Most postoperative surgical patients routinely receive supplemental oxygen therapy to prevent the potential development of hypoxemia due to incomplete lung re-expansion, reduced chest wall, and diaphragmatic activity caused by surgical site pain, consequences of hemodynamic impairment, and residual effects of anesthetic drugs (most notably residual neuromuscular blockade), which may result in atelectasis, ventilation–perfusion mismatch, alveolar hypoventilation, and impaired upper airway patency. Additionally, the World Health Organization guidelines for reducing surgical site infection have recommended the perioperative administration of high-dose oxygen, including during the immediate postoperative period. However, supplemental oxygen and hyperoxemia also have harmful effects on the respiratory and cardiovascular systems, with several clinical studies having reported an association between high perioperative oxygen administration and worse clinical outcomes. Recently, the increased availability of new and short-acting anesthetic drugs, comprehensive pharmacological knowledge, postoperative multimodal analgesia, and new minimally invasive surgery options could result in lower incidences of postoperative hypoxemia. Moreover, recommendations promoting high oxygen administration to prevent surgical site infections have been challenged, considering the lack of scientific investigations, and have not been widely accepted. Given the potential harmful effects of hyperoxemia, routine postoperative oxygen administration might not be recommended. Recent clinical studies have indicated that a conservative approach to oxygen therapy, where oxygen administration is titrated to achieve slightly lower oxygen levels than usual, could be safely implemented and decrease acutely ill patients’ susceptibility to hyperoxemia. Based on current evidence, appropriate monitoring, including peripheral oxygen saturation, and oxygen titration should be required during postoperative oxygen administration to avoid both hypoxemia and hyperoxemia. Future trials should therefore focus on determining the optimal oxygen target during postoperative care.
Autogenous bone augmentation from the zygomatic alveolar crest: a volumetric retrospective analysis in the maxilla
Authors: Kuster Irina , Osterwalder Livia , Valdec Silvio , Stadlinger Bernd , Wagner Maximilian E. H. , Rücker Martin , Bichsel Dominique
Published: October 2020

Background

Autogenous bone augmentation is the gold standard for the treatment of extended bone defects prior to implantation. Bone augmentation from the zygomatic crest is a valuable option with several advantages, but the current literature for this treatment is scant. The aim of this study was to evaluate the increase in bone volume after locoregional bone augmentation using autogenous bone from the zygomatic alveolar crest as well as the complications and success rate.

Results

Analysis of the augmented bone volume in seven patients showed a maximum volume gain of 0.97 cm3. An average of 0.54 cm3 of autogenous bone (SD 0.24 cm3; median: 0.54 cm3) was augmented. Implantation following bone augmentation was possible in all cases. Complications occurred in three patients.

Conclusion

The zygomatic alveolar crest is a valuable donor site for autogenous alveolar onlay grafting in a locoregional area such as the maxillary front. Low donor site morbidity, good access, and its suitable convexity make it a beneficial choice for autogenous bone augmentation.
Enhancer of mRNA Decapping protein 4 (EDC4) interacts with replication protein a (RPA) and contributes to Cisplatin resistance in cervical Cancer by alleviating DNA damage
Authors: Wu Xiaoling , Zhong Youwen , Chen Qing , Zhang Xin , Zhang Hua
Published: October 2020

Background

Cervical cancer (CC) is the third most common gynecological malignancy around the world. Cisplatin is an effective drug, but cisplatin resistance is a vital factor limiting the clinical usage of cisplatin. Enhancer of mRNA decapping protein 4 (EDC4) is a known regulator of mRNA decapping, which was related with genome stability and sensitivity of drugs. This research was to investigate the mechanism of EDC4 on cisplatin resistance in CC. Two human cervical cancer cell lines, HeLa and SiHa, were used to investigate the role of EDC4 on cisplatin resistance in vitro. The knockdown or overexpression of EDC4 or replication protein A (RPA) in HeLa or SiHa cells was performed by transfection. Cell viability was analyzed by MTT assay. The growth of cancer cells was evaluated by colony formation assay. DNA damage was measured by γH2AX (a sensitive DNA damage response marker) immunofluorescent staining. The binding of EDC4 and RPA was analyzed by immunoprecipitation.

Results

EDC4 knockdown in cervical cancer cells (HeLa and SiHa) enhanced cisplatin sensitivity and cisplatin induced cell growth inhibition and DNA damage. EDC4 overexpression reduced DNA damage caused by cisplatin and enhanced cell growth of cervical cancer cells. EDC4 could interact with RPA and promote RPA phosphorylation. RPA knockdown reversed the inhibitory effect of EDC4 on cisplatin-induced DNA damage.

Conclusion

The present results indicated that EDC4 is responsible for the cisplatin resistance partly through interacting with RPA in cervical cancer by alleviating DNA damage. This study indicated that EDC4 or RPA may be novel targets to combat chemotherapy resistance in cervical cancer.

Graphical abstract
Bromodomain-containing protein 4 regulates interleukin-34 expression in mouse ovarian cancer cells
Authors: Han Nanumi , Anwar Delnur , Hama Naoki , Kobayashi Takuto , Suzuki Hidefumi , Takahashi Hidehisa , Wada Haruka , Otsuka Ryo , Baghdadi Muhammad , Seino Ken-ichiro
Published: October 2020

Background

Interleukin (IL)-34 acts as an alternative ligand for the colony-stimulating factor-1 receptor and controls the biology of myeloid cells, including survival, proliferation, and differentiation. IL-34 has been reported to be expressed in cancer cells and to promote tumor progression and metastasis of certain cancers via the promotion of angiogenesis and immunosuppressive macrophage differentiation. We have shown in our previous reports that targeting IL-34 in chemo-resistant tumors in vitro resulted in a remarkable inhibition of tumor growth. Also, we reported poor prognosis in patients with IL-34-expressing tumor. Therefore, blocking of IL-34 is considered as a promising therapeutic strategy to suppress tumor progression. However, the molecular mechanisms that control IL-34 production are still largely unknown.

Methods

IL-34 producing ovarian cancer cell line HM-1 was treated by bromodomain and extra terminal inhibitor JQ1. The mRNA and protein expression of IL-34 was evaluated after JQ1 treatment. Chromatin immunoprecipitation was performed to confirm the involvement of bromodomain-containing protein 4 (Brd4) in the regulation of the Il34 gene. Anti-tumor effect of JQ1 was evaluated in mouse tumor model.

Results

We identified Brd4 as one of the critical molecules that regulate Il34 expression in cancer cells. Consistent with this, we found that JQ1 is capable of efficiently suppressing the recruitment of Brd4 to the promotor region of Il34 gene. Additionally, JQ1 treatment of mice bearing IL-34-producing tumor inhibited the tumor growth along with decreasing Il34 expression in the tumor.

Conclusion

The results unveiled for the first time the responsible molecule Brd4 that regulates Il34 expression in cancer cells and suggested its possibility as a treatment target.
The Treatment-induced Neuropathy Assessment Scale (TNAS): a psychometric update following qualitative enrichment
Authors: Mendoza Tito R. , Williams Loretta A. , Shi Qiuling , Wang Xin Shelley , Bamidele Oluwatosin , Woodruff Jeanie F. , Cleeland Charles S.
Published: October 2020

Background

The validation of the Treatment-induced Neuropathy Assessment Scale (TNAS v2.0), a patient-reported outcome measure of symptoms associated with cancer treatment-induced peripheral neuropathy (TIPN), was previously reported. Further patient input (qualitative interviewing, cognitive debriefing) suggested that the measure should be modified to better reflect the TIPN experience. We report the performance of a revised version (TNAS v3.0) for assessing TIPN across cancer treatments. This TNAS version incorporates extensive patient input, in accordance with FDA guidance on the development of patient-reported outcomes measures. Patients with multiple myeloma, colorectal cancer, or gynecological cancer treated with bortezomib, oxaliplatin, or taxane–platinum combination therapy, respectively, completed the TNAS v3.0, European Organization for Research and Treatment of Cancer Chemotherapy-Induced Peripheral Neuropathy (EORTC-CIPN20), and a cognitive debriefing survey during a scheduled clinic visit. Patients also participated in in-depth qualitative interviews about their TIPN symptoms. The psychometric properties of the TNAS v3.0 were evaluated.

Results

Cognitive debriefing survey results were summarized and showed that most patients found the items easy to complete, comprehensible, acceptable, and not redundant. A notable change from TNAS v2.0 was the separation of “numbness” from “tingling,” although these 2 items remained the most severe, followed by a new “pain” item. The Cronbach coefficient alphas for the 9-item TNAS were 0.88 and 0.90 at the first and second administrations, respectively, indicating good reliability. The test–retest reliability of the TNAS was 0.97. The correlation coefficients for the 9-item TNAS and the EORTC-CIPN20 were 0.69 for the sensory subscale, 0.70 for the motor subscale, and 0.32 for the autonomic subscale, indicating good validity.

Conclusion

This psychometric evaluation showed that the TNAS v3.0 is valid and reliable. Further research is needed to determine clinically meaningful differences in TNAS v3.0 scores and demonstrate its responsiveness over time.
E-consults: an effective way to decrease clinic wait times in rheumatology
Authors: Patel Veena , Stewart Diana , Horstman Molly J.
Published: October 2020

Background

To evaluate the effect of E-consults on wait times and resource utilization for positive antinuclear antibody (ANA) referrals in outpatient rheumatology.

Methods

We conducted a pre-post study of E-consult implementation for positive ANA referrals. We retrospectively reviewed “positive ANA” referrals from 1/2015–3/2017. A statistical process control chart was created to display monthly average wait times for in-person clinic visits and to identify special cause variation. Final diagnoses, wait times and resource utilization were recorded and compared between E-consults and in-person referrals.

Results

There were 139 referrals for positive ANA with 126 occurring after E-consult implementation in August 2015. Forty-four percent (55/126) of referrals were E-consults; 76% did not have an in-person visit after initial electronic rheumatology recommendation. A control chart demonstrated special cause variation in the form of a shift from June 2016 – January 2017, suggesting a temporal association between decreased wait times and the implementation of E-consults. Eleven patients were diagnosed with ANA-associated rheumatic disease; the majority of patients (73%, 86/139) did not have a rheumatologic diagnosis. Overall E-consults utilized more labs than in-person visits, but this was not statistically significant. In-person visits utilized more imaging studies, which was statistically significant.

Conclusion

E-consults are an effective way to address positive ANA consults without significant increase in resource utilization and were temporally associated with decreased wait times for in-person visits.
Life table and demographic parameters of the coccinellid predatory species, Hippodamia convergens Guérin-Méneville (Coleoptera: Coccinellidae) when fed on two aphid species
Authors: Arshad Muhammad , Ullah Muhammad Irfan , Shahid Umair , Tahir Muhammad , Khan Malik Imran , Rizwan Muhammad , Abrar Muhammad , Niaz Muhammad Mubashar
Published: October 2020

The study aimed to determine the effect of two aphid species; Lipaphis erysimi (Kaltenbach) and Aphis gossypii Glover (Hemiptera: Aphididae) on life table and demographic parameters of Hippodamia convergens Gu`erin-M´eneville (Coleoptera: Coccinellidae). The developmental period of all life stages of H. convergens was shorter when fed on L. erysimi. Net reproductive rate (Ro) was higher (159.85 offspring/individual) on L. erysimi, while it was 87.85 offspring/individual on A. gossypii. Mean generation time (T) was shorter (30.49 d) on L. erysimi than on A. gossypii (45.1 days). Values of the intrinsic rate of increase (r) and finite rate of increase (λ) were calculated as 0.166 days−1 and 1.181 days−1, respectively, on L. erysimi that were higher than on A. gossypii (0.099 days−1 and 1.104 days−1, respectively). Similarly, the survival rate was also higher when the predator was fed on L. erysimi. Overall, the findings suggest using L. erysimi as a better source of prey than A. gossypii for rearing H. convergens.
Lentiform fork sign in a uremic patient with a high anion gap metabolic acidosis with seizures: a case report from North West of Ireland
Authors: Mansoor Salman , De Klerk Lize , Lineen James , Fahad Muhammad , Ali Imran , Coffey Barry , Mulry Monica Ann , Saadat Shoab , Kelly Siobhan , Adenan Mohammad Hijaz , Murphy Kevin
Published: October 2020

Background

Lentiform fork sign is a neuroradiological abnormality which is encountered in the clinical practice associated with uremic encephalopathy, dialysis disequilibrium syndrome and metabolic acidosis.

Case presentation

We describe here a case of this neuro-radiological abnormality which was encountered in a patient with uraemia and high anion gap metabolic acidosis who presented with generalised convulsion and later had some tremor in her hands. In our patient, there were few predisposing factors which might have possibly resulted in this abnormality chronic kidney disease, diabetes mellitus, and metabolic acidosis.

Conclusion

The Lentiform fork sign is a rare occurrence which can be related to a long list of toxic and metabolic causes but in conjunction with metabolic acidosis in chronic kidney disease patients, it can narrow down this list of alternate diagnosis.
Effect of contamination sources on the rate of zinc, copper and nickel release from various soil ecosystems
Authors: Mansour Hesham , Awad Fikry , Saber Mohamed , Zaghloul Alaa
Published: October 2020

Background and objective

Soil is a substantive component in biosphere habitually endangered to superfluity contaminants particularly potential toxic elements (PTEs). The source of soil contaminants is very critical in controlling both their release and expected hazards in the different soil ecosystems. This study aims to investigate the effect of low-quality irrigation water (LQW) on the extent of soil pollution through desorption of zinc (Zn), nickel (Ni) and copper (Cu) from different polluted soils, collected from LQW irrigated farms for more than 40–80 years at Giza and Kafr-Elsheikh Governorates, Egypt.

Results

Models incorporated modified Freundlich (MFE), Elovich, first order and parabolic diffusion (PDE) showed significant results in describing the kinetic data under Egyptian conditions. Results entailed that according to the coefficient of determination (R2) and standard error, all used models well described the desorption of Cu, Ni and Zn, the most prevalent contaminants in the trailed soil ecosystems; for example, the R2 values were higher than 0.96** for zinc desorption using MFE model. As far as the PTEs levels are considered, the highest contaminant desorption rates were recorded at Kafr-Elsheikh site, followed by Kombora, and the lowest ones were in Abo Rawash soil ecosystem. The succession of more than one model to describe the kinetic perspective confirmed that the different mechanisms take place in PTEs sorption, distribution and subsequently release from different soil ecosystems.

Conclusions

The numerical values indicated that the soil ecosystems contaminated with industrial effluents were higher than those irrigated with sewage effluents regardless of the type of land use. More attention should be paid to low-quality water application in agriculture irrigation and its environmental risks.
Bismuth nanoparticles obtained by a facile synthesis method exhibit antimicrobial activity against Staphylococcus aureus and Candida albicans
Authors: Vazquez-Munoz Roberto , Arellano-Jimenez M. Josefina , Lopez-Ribot Jose L.
Published: October 2020

Background

Bismuth compounds are known for their activity against multiple microorganisms; yet, the antibiotic properties of bismuth nanoparticles (BiNPs) remain poorly explored. The objective of this work is to further the research of BiNPs for nanomedicine-related applications. Stable Polyvinylpyrrolidone (PVP)-coated BiNPs were produced by a chemical reduction process, in less than 30 min.

Results

We produced stable, small, spheroid PVP-coated BiNPs with a crystalline organization. The PVP-BiNPs showed potent antibacterial activity against the pathogenic bacterium Staphylococcus aureus and antifungal activity against the opportunistic pathogenic yeast Candida albicans, both under planktonic and biofilm growing conditions.

Conclusions

Our results indicate that BiNPs represent promising antimicrobial nanomaterials, and this facile synthetic method may allow for further investigation of their activity against a variety of pathogenic microorganisms.