bluuebunny/crossref_metadata_2025 huggingface dataset:
Chamas e Manchas
Authors: Esquivel Talita Gabriela Robbes
Published: October 2019

O presente texto consiste no memorial descritivo dos trabalhos realizados na disciplina Meios de Produção e Práticas Híbridas, ministrada pelo Prof. Dr. Agnus Valente, do curso de Pós-Graduação em Artes da UNESP, adaptado para participação na Jornada de pesquisa da UNESP, com o título Manchas de calor, com pequenas alterações para esta publicação da Revista Apotheke. Trata-se de trabalhos realizados por meio da queima, em forno à lenha, de manequins plásticos sobre suportes de metal. A produção girou em torno do calor e do formato do corpo, resultando em manchas nas placas de metal. O texto, em ordem cronológica, foca no processo criativo e abrange a produção física dos trabalhos e seus resultados. Isto é, discorre sobre o modo como foram feitos e sobre os resultados visuais dos trabalhos.
O princípio do mal na obra de Gabriel Garavello
Authors: Born Rodrigo Montandon , Marques Tatiana Lee
Published: October 2019

O princípio do mal na obra de Gabriel Garavello
Escola de postura apoiada na tecnologia assistiva e na terapia cognitiva-comportamental em dor lombar crônica
Authors: Vieira Sandro Emílio , Silva José Carlos Tavares da
Published: October 2019

Reconhecidamente de alta incidência, importante morbidade e com recidiva frequente, a dor lombar se faz presente no cotidiano de uma grande parcela da população mundial. De etiologia variada e com consequências físicas, psicoemocionais e sociais, a lombalgia crônica requer atenção ampliada, diante do seu tratamento. Para este estudo, a metodologia utilizada é de um ensaio clínico aleatório, com objetivo geral de implementar o conceito de Escola de Postura, utilizando Tecnologia Assistiva com ênfase em modelo cognitivo para o tratamento de quadros crônicos de lombalgias. A amostra foi de 20 participantes com dor lombar crônica, divididos em Grupo Experimental (GE) e Grupo Controle (GC), com alocação aleatória. Utilizaram-se instrumentos de avaliação nos períodos anterior e posterior a aplicação da abordagem experimental. A abordagem cognitiva foi aplicada no GE através de aulas virtuais relacionadas a aspectos cognitivos da lombalgia crônica. A análise dos dados apoiou-se nos escores das médias das avaliações, e foram discutidos relacionando seus resultados, que se apresentaram relevantes, porém não significativos, com redução das médias das variáveis no GE. Conclui-se, então, neste trabalho, que a implementação da Escola de Postura, com enfoque cognitivo através da Tecnologia Assistiva obteve resultados positivos para o GE nos indicadores dos aspectos psicossociais e físicos.
Sobre la conservación del fondo antiguo en bibliotecas españolas
Authors: Herrera José Luis
Published: October 2019

Se presentan los resultados del cuestionario pasado a 15 bibliotecas universitarias españolas sobre la gestión de la conservación de sus colecciones de fondo antiguo. El cuestionario ha sido diseñado para esta investigación y refleja los aspectos esenciales relacionados con la conservación. Se ha partido del estudio de los principios, pautas, estándares, etc. que guían la conservación y preservación de colecciones. Con los datos obtenidos se describe de manera global los rasgos principales de la conservación de las colecciones de fondo antiguo en las bibliotecas universitarias y se comparan con dos directrices de referencia.

The results of a questionnaire about the preservation of old collections are studied. The questionnaire has been answered by 23 Spanish university libraries. The questionnaire has been designed for this research, and reflects the essential aspects related to preservation. We have consulted principles, guidelines, standards, etc. that guide the conservation and preservation of collections. With the data obtained the preservation of the collections in the university libraries is described and compared with two reference guidelines. Improvement proposals are indicated.
Metodología para la mejora arquitectónica de repositorios universitarios
Authors: Fernández-Luna Adrià , Pérez-Montoro Mario , Guallar Javier
Published: October 2019

Se propone la utilización de metodologías propias de la disciplina de la Arquitectura de la información para mejorar el acceso a los contenidos de los repositorios universitarios o institucionales. Se ha observado que en la mayoría (80%) de los repositorios universitarios se utiliza una implementación por defecto del software DSpace. El hecho de no adecuar la arquitectura del repositorio a las necesidades de la universidad y sus usuarios puede generar una mala experiencia de usuario llegando a generar búsquedas insatisfactorias en una de cada tres interacciones. Este obstáculo en la recuperación de documentos puede evolucionar en una falta de visibilidad de la producción científica de la institución. Por ello se hace una propuesta metodológica para mejorar la arquitectura de los repositorios basada en las técnicas de User Persona y de Card Sorting empleando herramientas gratuitas como Google Forms, y se aplica la metodología a un estudio de caso: el Dipòsit Digital de la UB.

The article proposes the use of methodologies of the discipline of Information Architecture to improve access to the contents of institutional repositories. It has been observed that in the majority (80%) of the university repositories a default implementation of the DSpace software is used. The fact of not adapting the architecture of the repository to the needs of the university and its users can generate a bad user experience, generating unsatisfactory searches in one of every three interactions. This obstacle in the recovery of documents can evolve in a lack of visibility of the scientific production of the institution. Therefore, a methodological proposal is made to improve the architecture of the repositories based on the techniques of User Persona and Card Sorting using free tools such as Google Forms, and this methodology is applied to a case study: Dipòsit Digital of the UB.
Opiniões, necessidades e preferências dos utilizadores das bibliotecas hospitalares
Authors: Saraiva Rosa , Frías José Antonio , Lopes Carlos
Published: October 2019

Se presentan los resultados de un estudio sobre las opiniones, necesidades e intereses de los usuarios de las bibliotecas hospitalarias de la zona centro de Portugal. Esta investigación se llevó a cabo con la finalidad de conocer si los recursos y servicios de las bibliotecas de los hospitales contribuyen a la calidad de los cuidados prestados a los pacientes, a la práctica de la investigación y a la formación continua de los profesionales. Para ello se llevó a cabo una encuesta a 121 usuarios de las bibliotecas de siete hospitales de la zona centro de Portugal. Los resultados ponen de manifiesto las fortalezas y debilidades de los servicios bibliotecarios, a menudo poco conocidos, y cuya valoración varía dependiendo del tipo de usuario y de las iniciativas desarrolladas por las propias bibliotecas.

The results of a study about the opinions, needs and interests of the users of the hospital libraries of the central area of Portugal are presented. This research was carried out with the purpose of knowing if the resources and services of the libraries of the hospitals contribute to the quality of the care provided to the patients, to the practice of the research and to the continuous training of the professionals. For this purpose, a survey was carried out to 121 users of the libraries of seven hospitals in the central area of Portugal. The results highlight the strengths and weaknesses of library services, often little known, and whose assessment varies depending on the type of user and the initiatives developed by the libraries themselves.
Developing a Model for the Establishment of Pre-Hospital Emergency Medicine Bases in the Northern Provinces of Iran
Authors: Ahmadi Dashtiyan Azadeh , Masoudi Asl Iravan , Riahi Leila , Mahmoodi Mahmood
Published: October 2019

BACKGROUND: The faster and more accurately Pre-Hospital Emergency Medicine as the first line of care and treatment is done, the less the mortality and disability rates are.AIM: The present study is an attempt to design a model for the establishment of pre-hospital emergency bases in the northern provinces of Iran.METHODS: This quantitative-qualitative (mixed-method) research was conducted in two parts: First, using the literature review, interviews with experts in the field, and a comparative study, the most important factors affecting the establishment were extracted. Second, the obtained data were employed to formulate the development model and to design the questionnaire. The required data for factor analysis were collected through a questionnaire distributed among 200 operational personnel in January 2018. The results were analyzed using confirmatory factor analysis and multiple regression.RESULTS: Five components were identified after the exploratory factor analysis and Varimax with an eigenvalue larger than 1. The effect coefficients calculated for human resources components, service speed, and information and communication system were 0.935, 0.765 and 0.752, respectively. The obtained goodness of fit was very close to one, indicating the one-dimensional strength of the model. The highest parameter estimation in this model was allocated to the human component as 0.935, which has a significant correlation with other components.CONCLUSIONS: In order to reduce the response time, more attention should be paid to the allocation of budget and organizational roles, education, participation from government departments, establishment of an independent medical emergency organization, and appropriate accessibility to reduce the rates of mortality and morbidity.
LIVING CONDITIONS OF FEMALE FARMERS IN AUSTRIA
Authors: QUENDLER Erika
Published: October 2019

Understanding living conditions is critical to the understanding of female farmers’way of life. They are fundamental to people’s lives and vary from person to person.Normatively speaking, a good life cannot be achieved without having good livingconditions. The term living conditions, either as a target of different politicalinterests, agendas and priorities or closely related to private spheres of life, refersto the circumstances surrounding an individual’s life. This paper: (I) aims to definethe multidimensional concept of living conditions, (II) considers the importance ofthe concept and its application, and (III) offers statistically proven insights into theliving conditions of female farmers in Austria. The analysis is based on select datafrom the female farmers’ surveys in Austria. All in all, the results reveal thedevelopment from 2006 to 2016. The areas examined (education, financialindependence, civic engagement, work-life balance, social network and jobsatisfaction) show a positive development but there is also room for improvement –especially in the cases of financial inclusion and work life balance. Moreover, thereis clearly a need to learn more about the living conditions of female farmers –especially in connection with their lifestyle, and other concepts such as quality oflife, social inclusion and standard of living. Finally this paper tries to elaborate onthe need for further research and future perspectives.
SOIL NITRATE AND ORGANIC CARBON IMPROVEMENT BY USE OF LEGUMINOUS PLANTS AS COVER CROPS IN AN ORGANIC OLIVE ORCHARD
Authors: REPULLO-RUIBÉRRIZ DE TORRES Miguel A. , ORDÓÑEZ-FERNÁNDEZ Rafaela , MORENO-GARCÍA Manuel , MÁRQUEZ-GARCÍA Javier , CARBONELL-BOJOLLO Rosa
Published: October 2019

The organic olive orchard represents about 0.5 Mio ha in EU and 36% are locatedin Spain. This production system implies an economic opportunity for rural areas.The use of cover crops between the rows of olive trees is an agricultural practicethat farmers are progressively adopting since they reduce soil erosion whileimprove soil quality. Due to an usual fertilisation strategy in organic farming is theuse of leguminous plants, three legumes (Vicia sativa, Vicia ervilia and Viciavillosa) used as cover crops were compared to study their capacity to protect thesoil and improve soil fertility during 4 years. Two soil managements wereconsidered after mowing cover crops: plant residues left on surface or incorporatedinto the soil. Soil nitrate and coverage were monitored monthly and soil organiccarbon (SOC) was analysed at the end of every growing season. Despite not beingthe best species to protect the soil, the coverage at the end of decomposition periodincreased by 32% in 4 years in the management without incorporation, providingvalues over 30% of cover. The soil nitrate recorded in May, when the demand forN by the olive tree is greater, increased in the study period by 70% with theresidues left on surface and by 50% when the residues were buried. A carbonsequestration rate of 1.08 Mg C ha-1 y-1 was reached by V. villosa withoutincorporation. Where residues were incorporated, V. sativa obtained the highestcarbon fixation with 1.21 Mg C ha-1 y-1.
MECHANIZED MANAGEMENT OF PRUNING RESIDUES IN SWEET CHESTNUT ORCHARDS
Authors: TOMASONE Roberto , CEDROLA Carla , PAGANO Mauro , ACAMPORA Andrea , SANTANGELO Enrico
Published: October 2019

In Italy, Dryocosmus kuriphilus is a major insect pest of chestnut orchards causinggall formation and significant yield losses. The use of the parasitoid wasp Torymussinensis is an effective biological control method that requires the appropriatemanagement of the pruning residues, to preserve the woody galls in which T.sinensis adults overwinter. An innovative tractor-pulled combined mechanicalshredder was tested for treating the residues, once the pruning operations arecompleted. The machine processes the residues in a single-step, picking-up theprunings from the orchard floor, shredding them to appropriate size, and collectingthem into a rear container. The shredded residues may then be concentrated into afew piles for the long-term field storage, until next spring. The aim of the studywas to evaluate the machinery’s operative performance, the quality of the shreddedbiomass and the impact on gall integrity. In the field trials, the machineryeffectively shredded the residues achieving an average operative capacity of 1.19ha∙h-1 and a work capacity of 1.79 t∙h-1. Moisture content, apparent bulk density,and particle size distribution of the shredded biomass were determined. Over 95%of wood chips were included in the 3.15-45 mm size range, making them suitablefor use in residential biomass furnaces. The calorific power of the chestnut residueswas similar to that reported for other hardwood species (18-20 MJ∙kg-1). Also, themechanical action of the shredder preserved at least two thirds of the winter galls,safeguarding the parasitoid’s life cycle.
NEW ROLE OF AGRICULTURAL EXTENSION AND ADVISORY SERVICES BASED ON CURRENT FINDINGS AND FURTHER COLLABORATION FOR IMPROVED NUTRITION
Authors: MYRONIUK Iryna , SYMOCHKO Tayisiya , BOYKO Nadiya
Published: October 2019

Nowadays, there are a number of projects tackling on challenges around which thisstudy is based. For instance, the ultimate goal of the current project SKIN is tocreate a permanent stakeholders’ association on short food supply chain (SFSC)that works on the joint economic growth of the agricultural sector through theexchange of local food practices and through coaching sessions stimulatinginnovation. It creates a European network of best practices in SFSC that addressesthe fragmentation of knowledge in the agricultural sector and supports bottom-upinnovation initiatives. No doubts, boosting innovation through that project in localareas will lead to economic growth in the regions. But, for its sustainabledevelopment, it is crucial to create the agricultural extension and advisory services(AEAS), particularly in EU countries and also to modify their role using bottom-upapproaches. Despite the fact that the role of AEAS in the EU countries istransforming in the last few years, from a technology transfer paradigm to ademand-driven model, there are still two challenges that should be tackled in theglobal agenda: 1) facilitate linking of local agricultural sector and nutrition; 2)build a sustainable network of advisors in the EU for improving knowledge flowsin national and regional agricultural knowledge and innovation systems (AKIS).According also to the findings of the SKIN project and to our recently developedconcept (FENIX), the launch of new initiatives will enable AEAS by gathering of alarge amount of information and knowledge from local areas and population,helping all types of stakeholders to improve health, environmental, and economicsectors in targeted regions.
ASSESSING GREENHOUSE GAS EXCHANGE OF AGRICULTURAL CROPS BY FLUX MEASUREMENTS IN THRACE PART OF TURKEY
Authors: ŞAYLAN Levent , ASLAN Toprak , ALTINBAŞ Nilcan , YEŞİLKÖY Serhan , ÇALDAĞ Barış , BAKANOĞULLARI Fatih
Published: October 2019

Agriculture plays an important role in the global greenhouse gas (GHG) budget andits cycle. CO2 is one of the most important greenhouse gases, and plants releaseCO2 into the atmosphere by respiration and sink it by photosynthesis from theatmosphere. In addition, soil has an essential role in this exchange. Unfortunately,studies on the measurement of greenhouse gases above agricultural crops ininternationally accepted methods are not sufficient, especially in developingcountries. Thus, it is a clear need to determine carbon exchange of agriculturalcrops and activities (sink and emission) by taking into consideration of the specificconditions such as climate, crop variety, soil etc. Eddy Covariance (EC) is one ofthe widely used micrometeorological methods in the world for flux measurementstudies. Developments in measurement and analysis by instruments have allowedthis method to be applied more by researchers for the studies on GHG exchange. Inthis research, carbon exchanges (sink and emission) of watermelon grown inAtatürk Soil, Water and Agricultural Meteorology Research Institute located in theThrace part of Turkey, was measured using the Eddy Covariance method. Finally,estimated gas exchange above crops will be presented.
ON QUANTUM CRYPTOGRAPHY
Authors: Jacimovski Stevo , Setrajcic Jovan , Lamovec Jelena
Published: October 2019

In the late twentieth century, human race entered the era ofinformation technology (IT). The IT industry, which deals with the production,processing, storage and transmission of information, has become an integralpart of the global economic system, a completely independent and significantsector of the economy. The dependence of the modern society on informationtechnologies is so great that omissions in information systems may lead tosignificant incidents. Telecommunications are the key information technologyindustry. However, information is very susceptible to various types of abuseduring transmission. The units for data storage and processing can bephysically protected from anyone wishing harm, but this does not hold truefor the communication lines that span hundreds or thousands of kilometersand are virtually impossible to protect. Therefore, the problem of informationprotection in the field of telecommunications is highly significant. Cryptology,particularly cryptography, deals with this issue. Quantum cryptography is arelatively new field ensuring safe communication between the sender and therecipient using the laws of quantum physics. This paper seeks to address theprinciples of the quantum distribution of a key for information encryption andthe fundamental problems arising from the execution.
ПРИВАТНА И/ИЛИ КОРПОРАТИВНА БЕЗБЈЕДНОСТ Да ли постоје концептуалне сличности и разлике?
Authors: Лалић Велибор , Ћеранић Предраг , Сикимић Милица
Published: October 2019

У раду се анализирају теоријско-концептуална одређењаприватне и корпоративне безбједности. На основу прегледа литературе са-гледавају се заједничке карактеристике и специфичности, те критички пре-испитује основаност третирања њихових концептуалних сличности и раз-личитости. Кључно питање је да ли оне (сличности и разлике) постоје илисе ради о једном те истом концепту. Типологија корпоративне безбједностиLippert-a и сарадника кориштена је као аналитички оквир за компарацијукорпоративне и приватне безбједности. Свака од пет димензија из типоло-гије упоређивана је и анализирана. Прво су приказани резултати истражи-вања Lippert-a и сарадника по наведеној типологији, затим jе типологија,односно тај аналитички оквир кориштен за анализу приватне безбједности.На кају је извршено упоређивање приватне и корпоративне безбједностикако би се сагледало у којим димензијама типологије постоје преклапањаи сличности, а у којима разлике. У том смислу основни закључак је да при-ватна безбједност и корпоративна безбједност су сродни појмови који имајузначајне заједничке карактеристике (lat. genus proximum), али и специфич-ности што их чине различитим једно од другог (lat. differentia specifica). Наоснову компаративне анализе може се закључити да приватна безбједности корпоративна безбједност јесу сродни али су и концептуално различитипојмови.
Incomplete Objects and Unfulfilled Desire: Hubert Selby Jr.’s Requiem for a Dream
Authors: Muniowski Łukasz
Published: October 2019

Sara Goldfarb and her son Harry, two central characters in Hubert Selby Jr.’s Requiem for a Dream, are both addicts. The objects of their desire, a television set and a bag of drugs, are of particular signifi cance because they cannot be enjoyed without a transmitter – an antenna and a syringe. The article presents these objects as incomplete and the desire attached to them as misplaced. What the characters are really looking for is something beyond, “a pound of pure” happiness. The world in Requiem for a Dream is purely physical, so only what is done to the body can be felt and understood by the characters. In the end, Sara and Harry both become incomplete like the objects they are pursuing.
A Relevance-Theoretic Perspective on (Im)politeness Issues in the CB Radio Discourse of Polish Drivers
Authors: Nowik-Dziewicka Ewa
Published: October 2019

This paper has two major objectives. The first objective is to present the phenomenon of CB radio discourse of Polish drivers and discuss its main features. The second goal is to analyse two of these features from the angle of the (im)politeness issues, using the perspective of Relevance Theory. The first part of the paper is devoted to a general discussion of the phenomenon under scrutiny: the socio-cultural background of the CB radio discourse as well as its basic linguistic features are presented. In the second part of the paper, I discuss Escandell-Vidal’s approach to politeness within Relevance framework (Escandell-Vidal 2004) and integrate the model with the habitus-based definition of politeness (Bourdieu 1977; Watts 2003). The final part of the paper shows how the integrated approach can be used to examine two major aspects of the CB radio discourse: its conversational structure and the use of diminutives.
The Framing of a Preferred Variety of English by Pre-Service Primary School Teachers of English as a Foreign Language
Authors: Kapranov Oleksandr
Published: October 2019

The article presents a mixed-method study on how the preferred variety of the English language was framed by pre-service primary school teachers of English as a Foreign Language (EFL). The group of pre-service primary school teachers (further referred to as “participants”) was recruited at a large university in Norway and matched with the respective control group of non-teacher students enrolled in the English course at the same university. The participants and controls were asked to write a reflective essay on their preferred variety of the English language. The corpus of the participants’ and controls’ essays was analysed quantitatively and qualitatively. The results of the quantitative analysis revealed that British English was preferred by 47% of the participants, who framed it via the frames “Films/TV”, “Sounds”, “Spelling”, “Teacher”, and “Visit”. Those findings were further discussed in the article.
Improved characterisation of MRSA transmission using within-host bacterial sequence diversity
Authors: Hall Matthew D , Holden Matthew TG , Srisomang Pramot , Mahavanakul Weera , Wuthiekanun Vanaporn , Limmathurotsakul Direk , Fountain Kay , Parkhill Julian , Nickerson Emma K , Peacock Sharon J , Fraser Christophe
Published: October 2019

Methicillin-resistant Staphylococcus aureus (MRSA) transmission in the hospital setting has been a frequent subject of investigation using bacterial genomes, but previous approaches have not yet fully utilised the extra deductive power provided when multiple pathogen samples are acquired from each host. Here, we used a large dataset of MRSA sequences from multiply-sampled patients to reconstruct colonisation of individuals in a high-transmission setting in a hospital in Thailand. We reconstructed transmission trees for MRSA. We also investigated transmission between anatomical sites on the same individual, finding that this either occurs repeatedly or involves a wide transmission bottleneck. We examined the between-subject bottleneck, finding considerable variation in the amount of diversity transmitted. Finally, we compared our approach to the simpler method of identifying transmission pairs using single nucleotide polymorphism (SNP) counts. This suggested that the optimum threshold for identifying a pair is 39 SNPs, if sensitivities and specificities are equally weighted.
Pretectal neurons control hunting behaviour
Authors: Antinucci Paride , Folgueira Mónica , Bianco Isaac H
Published: October 2019

For many species, hunting is an innate behaviour that is crucial for survival, yet the circuits that control predatory action sequences are poorly understood. We used larval zebrafish to identify a population of pretectal neurons that control hunting. By combining calcium imaging with a virtual hunting assay, we identified a discrete pretectal region that is selectively active when animals initiate hunting. Targeted genetic labelling allowed us to examine the function and morphology of individual cells and identify two classes of pretectal neuron that project to ipsilateral optic tectum or the contralateral tegmentum. Optogenetic stimulation of single neurons of either class was able to induce sustained hunting sequences, in the absence of prey. Furthermore, laser ablation of these neurons impaired prey-catching and prevented induction of hunting by optogenetic stimulation of the anterior-ventral tectum. We propose that this specific population of pretectal neurons functions as a command system to induce predatory behaviour.
Stereotyped transcriptomic transformation of somatosensory neurons in response to injury
Authors: Nguyen Minh Q , Le Pichon Claire E , Ryba Nicholas
Published: October 2019

In mice, spared nerve injury replicates symptoms of human neuropathic pain and induces upregulation of many genes in somatosensory neurons. Here we used single cell transcriptomics to probe the effects of partial infraorbital transection of the trigeminal nerve at the cellular level. Uninjured neurons were unaffected by transection of major nerve branches, segregating into many different classes. In marked contrast, axotomy rapidly transformed damaged neurons into just two new and closely-related classes where almost all original identity was lost. Remarkably, sensory neurons also adopted this transcriptomic state following various minor peripheral injuries. By genetically marking injured neurons, we showed that the injury-induced transformation was reversible, with damaged cells slowly reacquiring normal gene expression profiles. Thus, our data expose transcriptomic plasticity, previously thought of as a driver of chronic pain, as a programed response to many types of injury and a potential mechanism for regulating sensation during wound healing.
MicroRNA-205-5p inhibits three-dimensional spheroid proliferation of ErbB2-overexpressing breast epithelial cells through direct targeting of CLCN3
Authors: Takeno Takayoshi , Hasegawa Takuya , Hasegawa Hiroki , Ueno Yasuyuki , Hamataka Ryo , Nakajima Aya , Okubo Junji , Sato Koji , Sakamaki Toshiyuki
Published: October 2019

We previously reported that microRNA-205-5p (miR-205-5p) is significantly decreased in the ErbB2-overexpressing breast epithelial cell line MCF10A-ErbB2 compared with control cells. In this study, we identified a direct target of miR-205-5p, chloride voltage-gated channel 3 (CLCN3). CLCN3 expression was induced by ErbB2 overexpression; this induced expression was then reduced to control levels by the transfection of the miR-205-5p precursor. In RNA-binding protein immunoprecipitation with Ago1/2/3 antibody, CLCN3 was significantly enriched in 293T embryonic kidney cells with miR-205-5p mimic transfection compared with negative control mimic transfection. In luciferase reporter assays using CLCN3 3′-UTR constructs, the miR-205-5p mimic significantly decreased reporter activity of both wild-type and partial mutant constructs in MCF10A-ErbB2 cells. In contrast, no inhibitory effects of the miR-205-5p mimic were detected using the complete mutant constructs. Since miR-205-5p expression in exosomes derived from MCF10A-neo cells was substantially higher than in exosomes derived from MCF10A-ErbB2 cells, we next investigated whether an exosome-mediated miR-205-5p transfer could control CLCN3 expression. To this end, exosomal miR-205-5p derived from MCF10A-neo cells was functionally transferred to MCF10A-ErbB2 cells, which served to decrease the expression of CLCN3. To assess the roles of CLCN3 in breast cancer, we next performed three-dimensional (3D) spheroid proliferation analyses using MCF10A-ErbB2 cells treated with MCF10A-neo-derived exosomes or CLCN3 shRNA stably expressing SKBR3 and MDA-MB-453 breast cancer cells. Our results showed that both treatment with MCF10A-neo-derived exosome and CLCN3 shRNA expression suppressed 3D spheroid proliferation. Collectively, these novel findings suggest that CLCN3 may be a novel direct target of miR-205-5p and this CLCN3/miR-205-5p interaction may serve a pivotal role in regulating breast cancer cellular proliferation under physiological conditions.
Endemism and diversity of small mammals along two neighboring Bornean mountains
Authors: Camacho-Sanchez Miguel , Hawkins Melissa T.R. , Tuh Yit Yu Fred , Maldonado Jesus E. , Leonard Jennifer A.
Published: October 2019

Mountains offer replicated units with large biotic and abiotic gradients in a reduced spatial scale. This transforms them into well-suited scenarios to evaluate biogeographic theories. Mountain biogeography is a hot topic of research and many theories have been proposed to describe the changes in biodiversity with elevation. Geometric constraints, which predict the highest diversity to occur in mid-elevations, have been a focal part of this discussion. Despite this, there is no general theory to explain these patterns, probably because of the interaction among different predictors with the local effects of historical factors. We characterize the diversity of small non-volant mammals across the elevational gradient on Mount (Mt.) Kinabalu (4,095 m) and Mt. Tambuyukon (2,579 m), two neighboring mountains in Borneo, Malaysia. We documented a decrease in species richness with elevation which deviates from expectations of the geometric constraints and suggests that spatial factors (e.g., larger diversity in larger areas) are important. The lowland small mammal community was replaced in higher elevations (from above ~1,900 m) with montane communities consisting mainly of high elevation Borneo endemics. The positive correlation we find between elevation and endemism is concordant with a hypothesis that predicts higher endemism with topographical isolation. This supports lineage history and geographic history could be important drivers of species diversity in this region.
The Satisfaction of Patients Assisted by a Health Education Ambulatory / Satisfação dos Pacientes Atendidos no Ambulatório de Educação em Saúde
Authors: Coutinho Ana Flávia Pereira , Lopes Juliana de Lima , Carneiro Camila de Souza , Santos Vinícius Batista , Barros Alba Lucia Bottura Leite de
Published: October 2019

Objetivo: Identificar a satisfação dos pacientes acompanhados em um Ambulatório de Educação em Saúde e avaliar o efeito das variáveis antecedentes com o grau de satisfação dos pacientes. Métodos: Trata-se de um estudo de corte transversal, realizado em um Ambulatório de Educação em Saúde. A amostra foi composta por todos os pacientes cardiopatas atendidos neste ambulatório (17 pacientes). A satisfação dos pacientes foi avaliada por meio do instrumento Satisfação do Paciente (ISP). Resultados: Observou-se que todos os pacientes relataram um alto nível de satisfação e os domínios que obtiveram maiores e menores pontuações foram o profissional e o educacional, respectivamente. Não houve correlação significativa do nível de satisfação com nenhuma das variáveis antecedentes. Conclusão: Os pacientes apresentaram alto nível de satisfação e nenhuma variável se relacionou com a satisfação.
Home Care Service and Health Care Networks / Serviço de Atenção Domiciliar e as Redes de Atenção à Saúde
Authors: Weykamp Juliana Marques , De Siqueira Hedi Crecencia Heckler , Cecagno Diana , De Medeiros Adriane Calvetti , De Paula Saul Ferraz , Pedroso Vanessa Soares Mendes
Published: October 2019

Objetivou-se conhecer a inter-relação do Serviço de Atenção Domiciliar nas modalidades 2 e 3 com as Redes de Atenção à Saúde nos municípios do Rio Grande do Sul. Método: Estudo descritivo, exploratório, qualitativo, realizado em 06 Serviços de Atenção Domiciliar no período de 23 à 30 de outubro de 2015. Participaram da pesquisa 04 coordenadores e 09 enfermeiros. A coleta de dados ocorreu por meio de entrevista semiestruturada e para análise utilizou-se a Análise de Conteúdo de Bardin. Resultados e discussão: A articulação das práticas em saúde, aliadas às Redes de Atenção à Saúde representam um elemento benéfico e inovador, possibilitando a prestação de uma assistência contínua e dinâmica para a população. Conclusão: O funcionamento do Serviço de Atenção Domiciliar e a dinâmica das redes necessitam estar organizada, esclarecida, articulada e desenvolvida em cooperação entre os serviços e instituições de saúde, visando a importância na reestruturação produtiva na saúde.
Experiences From The Puerperal Period According to The Viewpoint of Adolescent Women / Vivências do Período Gravídico-Puerperal na Perspectiva de Mulheres Adolescentes
Authors: Cremonese Luiza , Wilhelm Laís Antunes , Demori Carolina Carbonell , Prates Lisie Alende , Barreto Camila Nunes , Ressel Lúcia Beatriz
Published: October 2019

Objetivo: conhecer como a mulher adolescente vivencia o período gravídico-puerperal. Método: Estudo qualitativo descritivo. As participantes foram 11 puérperas adolescentes. Para coleta de dados utilizou-se a entrevista semiestruturada e o mapa falante. Os dados foram submetidos à análise de conteúdo temática da proposta operativa. Resultados: Revelaram que no início da gestação sentiram insegurança, medo e rejeição; passaram por alguns abandonos e afastamentos; tiveram que reorganizar os planos de vida; os estudos foram adiados; mas, ao final, prevaleceu o sentimento de felicidade ao ter o filho nos braços. Conclusão: conhecer a vivência do período gravídico-puerperal das adolescentes permitiu compreender a importância do profissional de saúde atuar neste contexto e poder contribuir para evolução de uma gestação saudável a partir da compreensão das singularidades das adolescentes.
The Nursing Students’ Viewpoint Regarding the Papanicolaou Test for Gynecological Diseases Diagnosis / Percepção dos Estudantes de Enfermagem Sobre o Exame Papanicolau para Diagnóstico das Doenças Ginecológicas
Authors: Souza Talita Araujo de , Leite Kamila Nethiely Souza , Medeiros Fabíola Kelly Formiga , Nunes Geisiani Sousa , De Sousa Kilmara Melo , César Erta Soraya Ribeiro
Published: October 2019

Objetivo: Desvelar a percepção dos estudantes de enfermagem sobre o exame Papanicolau no diagnóstico das doenças ginecológicas. Métodos: descritivo e exploratório, com abordagem quali-quantitativa. A pesquisa foi realizada nas Faculdades Integradas de Patos no curso de enfermagem. A amostra composta por 100 estudantes de enfermagem que aceitaram participar da pesquisa. Resultados: Todos os estudantes sabem da eficácia do exame, porém nem todos tiveram a oportunidade de realizar o mesmo durante os estágios, além disso, nota-se que os estudantes sabem a importância do exame Papanicolau e as orientações necessárias para a saúde da mulher. Conclusão: Evidenciou-se a importância do conhecimento téorico e prático dos estudantes e futuros profissionais da enfermagem, sobre a conduta do exame e orientações a serem repassadas para as usuarias que venham a realizar o papanicolau.
Interfering Factors of the Breastfeeding Process in Children Bearing Various Health Needs: Contributions to Nursing / Fatores que Interferem no Processo de Aleitamento Materno de Crianças com Necessidades de Saúde Variadas: Contribuições Para A Enfermagem
Authors: Falsett Carolina Fernandes , Santos Inês Maria Meneses dos , Vasconcellos Aline Martins
Published: October 2019

Objetivo: Analisar os fatores que influenciaram o processo de aleitamento materno de crianças acompanhadas em ambulatório de referência na visão da mulher-mãe. Método: Estudo descritivo com abordagem qualitativa, aprovada pelo Comitê de Ética em Pesquisa, parecer nº 1370267. Realizado em um Hospital Universitário do Estado do Rio de Janeiro, com 30 mulheres-mães de crianças que vivenciaram o processo de amamentação, cujos filhos tinham até 24 meses. Os dados foram coletados de março a abril de 2016, por entrevista semiestruturada guiada por formulário. Resultados: Nos relatos das mulheres-mães apreendeu-se como fatores positivos: vínculo afetivo e conhecimentos sobre amamentação, e como negativos: problemas com a amamentação, problemas socioafetivos e patologia da criança. Conclusões: Foi constatado que todas as mulheres-mães foram orientadas em algum momento, e as que relataram fatores negativos, nem todas interromperam o processo de aleitamento, algumas superaram as dificuldades e deram continuidade. Descritores: Saúde da criança; Enfermagem Pediátrica; Aleitamento materno.
Experiences of Mothers During The Hospitalizatrion of Their Children / Experiências de Mães Durante a Internação Hospitalar de Seus Filhos
Authors: Lima Roseni Medeiro , Gomes Francisco Meykel Amâncio , Aguiar Francisca Alanny Rocha , Santos Júnior Edson Batista Dos , Dourado João Victor Lira , Ferreira Junior Antonio Rodrigues
Published: October 2019

Objetivo: conhecer a experiência de mães durante a internação hospitalar de seus filhos. Métodos: Estudo descritivo-exploratório, com abordagem qualitativa em uma clínica pediátrica de um Hospital de referência na região norte do Ceará, Brasil, em novembro de 2015, com oito mães com filhos internados há pelo menos sete dias. A coleta das informações ocorreu por meio de entrevistas semiestruturada individual, que foram organizadas por meio da análise temática. Resultados: Configuraram-se quatro categorias temáticas: experiência de mães com filhos em internação hospitalar; contribuições das mães na recuperação do filho; qualidade do atendimento de enfermagem na percepção das mães e; sentimentos despertados das mães durante a internação hospitalar do filho. Conclusão: Evidenciou-se a necessidade de a equipe de saúde planejar e implementar cuidados de saúde qualificados, e que esses estejam imbuídos de humanização e de uma perspectiva de atenção integral.
Social Support Network for the Family of Cerebral Palsy Bearing Children / Rede De Apoio Social À Família Da Criança Com Paralisia Cerebral
Authors: Alves dos Santos Bruna , Marten Milbrath Viviane , Freitag Vera Lúcia , Irmgard Bärtschi Gabatz Ruth , Cardoso Vaz Jéssica
Published: October 2019

Objetivo: Conhecer as redes de apoio social das famílias de crianças com paralisia cerebral. Métodos: Estudo qualitativo, exploratório e descritivo, realizado na Associação de Pais e Amigos dos Excepcionais, de um município situado no sul do Brasil, com mães e pais de crianças com paralisia cerebral. A coleta das informações ocorreu entre abril e maio/2017, por meio de entrevistas. Foram respeitados todos os preceitos éticos estabelecidos pela resolução nº. 466/12. Resultados: Constatou-se que a rede social é composta pela família e, principalmente, pelos profissionais de saúde e de assistência social em instituições de referência como a Associação de Pais e Amigos dos Excepcionais. Conclusão: As redes de apoio são imprescindíveis para que as famílias de crianças com paralisia cerebral possam cuidar adequadamente de seus filhos. Os profissionais de saúde podem prestar orientações sobre as necessidades da criança e suporte para o cuidado familiar.
The application of omics-based human liver platforms for investigating the mechanism of drug-induced hepatotoxicity in vitro
Authors: Jiang Jian , Pieterman Charlie D. , Ertaylan Gökhan , Peeters Ralf L. M. , de Kok Theo M. C. M.
Published: October 2019

Drug-induced liver injury (DILI) complicates safety assessment for new drugs and poses major threats to both patient health and drug development in the pharmaceutical industry. A number of human liver cell-based in vitro models combined with toxicogenomics methods have been developed as an alternative to animal testing for studying human DILI mechanisms. In this review, we discuss the in vitro human liver systems and their applications in omics-based drug-induced hepatotoxicity studies. We furthermore present bioinformatic approaches that are useful for analyzing toxicogenomic data generated from these models and discuss their current and potential contributions to the understanding of mechanisms of DILI. Human pluripotent stem cells, carrying donor-specific genetic information, hold great potential for advancing the study of individual-specific toxicological responses. When co-cultured with other liver-derived non-parenchymal cells in a microfluidic device, the resulting dynamic platform enables us to study immune-mediated drug hypersensitivity and accelerates personalized drug toxicology studies. A flexible microfluidic platform would also support the assembly of a more advanced organs-on-a-chip device, further bridging gap between in vitro and in vivo conditions. The standard transcriptomic analysis of these cell systems can be complemented with causality-inferring approaches to improve the understanding of DILI mechanisms. These approaches involve statistical techniques capable of elucidating regulatory interactions in parts of these mechanisms. The use of more elaborated human liver models, in harmony with causality-inferring bioinformatic approaches will pave the way for establishing a powerful methodology to systematically assess DILI mechanisms across a wide range of conditions.
Personalised Warfarin Dosing in Children Post-cardiac Surgery
Authors: Al-Metwali Basma Zuheir , Rivers Peter , Goodyer Larry , O’Hare Linda , Young Sanfui , Mulla Hussain
Published: October 2019

Warfarin dosing is challenging due to a multitude of factors affecting its pharmacokinetics (PK) and pharmacodynamics (PD). A novel personalised dosing algorithm predicated on a warfarin PK/PD model and incorporating CYP2C9 and VKORC1 genotype information has been developed for children. The present prospective, observational study aimed to compare the model with conventional weight-based dosing. The study involved two groups of children post-cardiac surgery: Group 1 were warfarin naïve, in whom loading and maintenance doses were estimated using the model over a 6-month duration and compared to historical case-matched controls. Group 2 were already established on maintenance therapy and randomised into a crossover study comparing the model with conventional maintenance dosing, over a 12-month period. Five patients enrolled in Group 1. Compared to the control group, the median time to achieve the first therapeutic INR was longer (5 vs. 2 days), to stable anticoagulation was shorter (29.0 vs. 96.5 days), to over-anticoagulation was longer (15.0 vs. 4.0 days). In addition, median percentage of INRs within the target range (%ITR) and percentage of time in therapeutic range (%TTR) was higher; 70% versus 47.4% and 83.4% versus 62.3%, respectively. Group 2 included 26 patients. No significant differences in INR control were found between model and conventional dosing phases; mean %ITR was 68.82% versus 67.9% (p = 0.84) and mean %TTR was 85.47% versus 80.2% (p = 0.09), respectively. The results suggest model-based dosing can improve anticoagulation control, particularly when initiating and stabilising warfarin dosing. Larger studies are needed to confirm these findings.
Correction to: Phase II study to evaluate the efficacy of Trastuzumab in combination with Capecitabine and Oxaliplatin in first-line treatment of HER2-positive advanced gastric cancer: HERXO trial
Authors: Rivera Fernando , Romero C. , Jimenez-Fonseca P. , Izquierdo-Manuel M. , Salud A. , Martínez E. , Jorge M. , Arrazubi V. , Méndez J. C. , García-Alfonso P. , Reboredo M. , Barriuso J. , Muñoz-Unceta N. , Jimeno R. , López C.
Published: October 2019

Phase II study to evaluate the efficacy of Trastuzumab in combination with Capecitabine.
Modelling collective cell migration: neural crest as a model paradigm
Authors: Giniūnaitė Rasa , Baker Ruth E. , Kulesa Paul M. , Maini Philip K.
Published: October 2019

A huge variety of mathematical models have been used to investigate collective cell migration. The aim of this brief review is twofold: to present a number of modelling approaches that incorporate the key factors affecting cell migration, including cell–cell and cell–tissue interactions, as well as domain growth, and to showcase their application to model the migration of neural crest cells. We discuss the complementary strengths of microscale and macroscale models, and identify why it can be important to understand how these modelling approaches are related. We consider neural crest cell migration as a model paradigm to illustrate how the application of different mathematical modelling techniques, combined with experimental results, can provide new biological insights. We conclude by highlighting a number of future challenges for the mathematical modelling of neural crest cell migration.
Different vascular healing process between bioabsorbable polymer-coated everolimus-eluting stents versus bioresorbable vascular scaffolds via optical coherence tomography and coronary angioscopy (the ENHANCE study: ENdothelial Healing Assessment with Novel Coronary tEchnology)
Authors: Wan Ahmad Wan Azman , Nakayoshi Takaharu , Mahmood Zuhdi Ahmad Syadi , Ismail Muhammad Dzafir , Zainal Abidin Imran , Ino Yasushi , Kubo Takashi , Akasaka Takashi , Fukumoto Yoshihiro , Ueno Takafumi
Published: October 2019

Recent clinical trials have raised concerns about the safety and efficacy of ABSORB™ bioresorbable vascular scaffolds (BVS). The difference in the vascular healing process between SYNERGY™ bioabsorbable polymer-coated everolimus-eluting stents (BP-EES) and BVS remains unclear. The aim of the ENHANCE study was to compare vascular healing on BP-EES versus BVS by optical coherence tomography (OCT) and coronary angioscopy (CAS) at 4- and 12-month follow-ups. This is a prospective, non-randomized, single center clinical trial. Thirteen eligible patients with multivessel disease were enrolled. BP-EES and BVS were simultaneously implanted in the same patients, but in different coronary vessels. Imaging follow-up with both OCT and CAS was completed in 11 patients at 12 months. Neointimal coverage rates were similar between the two groups based on OCT measurements. The neointimal thickness of BP-EES was significantly thicker at the 12th month than at the 4th month, whereas the neointimal thickness of BVS did not change between the measurements taken at the 4th and 12th month. Existence of intra-stent thrombus was significantly higher in the BVS group, compared to the BP-EES group. On the other hand, CAS revealed that red-thrombi and yellow-plaque were more frequently observed in BVS at 4 months and up to 12-month follow-ups than in BP-EES. These findings suggested that the evidence of instability remained up to 12 months in the vascular healing with BVS, compared to that with BP-EES. Vascular healing of the stented wall was recognized at the very early phase after BP-EES implantation. However, vascular healing with BVS was still incomplete after 12 months.