bluuebunny/crossref_metadata_2025 huggingface dataset:
Expanded Longitudinal Deformation Profile in Tunnel Excavations Considering Rock Mass Conditions via 3D Numerical Analyses
Authors: Ha Sang-gui , Naji Abdul Muntaqim , Rehaman Hafeezur , Nam Kyoung-min , Kim Han-eol , Park Jae-won , Yoo Han-kyu
Published: June 2021

In the convergence–confinement method, the longitudinal deformation profile (LDP) serves as a graphical representation of the actual tunnel convergence (both ahead of and behind the face); therefore, it is considered important for determining the distance of support installation from the face or the timing after excavation in this method. The LDP is a function of the rock mass quality, excavation size, and state of in situ stresses; thus, obtaining the LDP according to the rock mass conditions is essential for analyzing the complete behavior of convergence during tunnel excavation. The famous LDP shows that the best fit for the measured values of tunnel internal displacement reported simply expresses the ratio of the preceding displacement as approximately 0.3. This can lead to an error when predicting the ratio of the preceding displacement while neglecting the rock conditions; consequently, a complete tunnel behavior analysis cannot be realized. To avoid such error, the finite difference method software FLAC 3D is used to develop an expanded longitudinal deformation profile (ELDP) according to the rock mass conditions. The ELDP is represented by graphs featuring different shapes according to the rock mass rating (RMR), and the empirical formula of the LDP best fitted for the tunnel convergence measurement values is expanded. This expanded LDP formula is proposed in a generalized form, including the parameters α and β from the empirical equation. These parameters α and β are expressed as functions of the RMR and initial stress. Statistical analysis results of the 3D numerical analysis of 35 cases were analyzed in the ranges of α = 0.898–2.416 and β = 1.361–2.851; this result is based on the empirical formula of Hoek (1999) (α = 1.1, β = 1.7), which was expanded in the current study according to the rock quality and initial stress conditions.
Hand Gesture Detection and Recognition Using Spectrogram and Image Processing Technique with a Single Pair of Ultrasonic Transducers
Authors: Feng Guo-Hua , Lai Gui-Rong
Published: June 2021

This paper presents an effective signal processing scheme of hand gesture recognition with a superior accuracy rate of judging identical and dissimilar hand gestures. This scheme is implemented with the air sonar possessing a pair of cost-effective ultrasonic emitter and receiver along with signal processing circuitry. Through the circuitry, the Doppler signals of hand gestures are obtained and processed with the developed algorithm for recognition. Four different hand gestures of push motion, wrist motion from flexion to extension, pinch out, and hand rotation are investigated. To judge the starting time of hand gesture occurrence, the technique based on continuous short-period analysis is proposed. It could identify the starting time of the hand gesture with small-scale motion and avoid faulty judgment while no hand in front of the sonar. Fusing the short-time Fourier transform spectrogram of hand gesture to the image processing techniques of corner feature detection, feature descriptors, and Hamming-distance matching are the first-time, to our knowledge, employed to recognize hand gestures. The results show that the number of matching points is an effective parameter for classifying hand gestures. Based on the experimental data, the proposed scheme could achieve an accuracy rate of 99.8% for the hand gesture recognition.
Oral Manifestations in Children and Young Adults with Down Syndrome: A Systematic Review of the Literature
Authors: Contaldo Maria , Santoro Rossella , Romano Antonio , Loffredo Francesca , Di Stasio Dario , Della Vella Fedora , Scivetti Michele , Petruzzi Massimo , Serpico Rosario , Lucchese Alberta
Published: June 2021

Down syndrome (DS) is an autosomal disorder associated with mental and physical involvement. The typical craniofacial phenotype and the dental anomalies in DS subjects have been widely described, but a systematic report on the manifestations affecting the oral mucosae in children with DS is still lacking. This systematic review aimed to establish the prevalence of oral mucosal manifestations in children/young adults with DS. Pubmed, Web of Science, and Scopus were investigated in September 2020. Documents in English on DS children/young adults (up to 25 years) reporting oral mucosal findings were considered. Study quality was assessed with ROBIN-I. Of the 150 references retrieved, 14 studies were considered eligible. The risk of bias ranged from low to unclear. Fissured tongue appeared to increase with age and was more prevalent in DS children than in the general population. Lip fissures and cheilitis were heterogeneously reported. Candida spp. carriage with and without active candidiasis was more frequent in DS children/young adults than in controls. C. albicans was the most prevalent species. Few other oral mucosal conditions have been reported sporadically. The heterogeneity of the works revealed the need for more appropriate oral examination to intercept the oral manifestations of oral mucosa and prevent recurrent candidiasis.
The Enhanced Naturally Occurring Radioactivity of Negative Ion Clothing and Attendant Risk
Authors: Hassan Halmat Jalal , Hashim Suhairul , Abu Hanifah Noor Zati Hani , Hadi Muhammad Fahmi Rizal Abdul , Sanusi Mohamad Syazwan Mohd , Bradley David Andrew , Tenorio Rafael García , Tahar Rozman Mohd
Published: June 2021

The study investigates commercially available negative ion clothing, and evaluations are made using gamma-ray spectroscopy and Geant4 Monte Carlo simulations. Observed to contain naturally occurring radioactive material (NORM), evaluations are made of the radiological risk arising from the use of these as items of everyday wear, undergarments in particular. Organ doses from these were simulated using the MIRD5 mathematical female phantom, with the incorporation of dose conversion factors (DCFs). At 175 ± 26, 1732 ± 247, and 207 ± 38 Bq, for 238U, 232Th, and 40K respectively, item code S05 was found to possess the greatest activity, while item code S07 was shown to have the least activity, at 2 ± 0.5 and 15 ± 2 Bq, and again for 238U and 232Th, respectively. Sample code S11 recorded least activity, at 29 ± 5 Bq, for 40K. Among the clothing items, sample item code S05 offered the greatest concentrations of Th, U and Zr, with percentage means of 1.23 ± 0.1, 0.045 ± 0.001, and 1.29 ± 0.1, respectively, giving rise to an annual effective dose of 1.57 mSv/y assuming a nominal wearing period of 24 h per day. Accordingly, the annual public dose limit of 1 mSv can be exceeded by their use.
ResQbot 2.0: An Improved Design of a Mobile Rescue Robot with an Inflatable Neck Securing Device for Safe Casualty Extraction
Authors: Saputra Roni Permana , Rakicevic Nemanja , Kuder Isabelle , Bilsdorfer Joel , Gough Alexander , Dakin Alexandra , de Cocker Emma , Rock Shaun , Harpin Richard , Kormushev Petar
Published: June 2021

Despite the fact that a large number of research studies have been conducted in the field of search and rescue robotics, significantly little attention has been given to the development of rescue robots capable of performing physical rescue interventions, including loading and transporting victims to a safe zone—i.e., casualty extraction tasks. The aim of this study is to develop a mobile rescue robot that could assist first responders when saving casualties from a dangerous area by performing a casualty extraction procedure whilst ensuring that no additional injury is caused by the operation and no additional lives are put at risk. In this paper, we present a novel design of ResQbot 2.0—a mobile rescue robot designed for performing the casualty extraction task. This robot is a stretcher-type casualty extraction robot, which is a significantly improved version of the initial proof-of-concept prototype, ResQbot (retrospectively referred to as ResQbot 1.0), that has been developed in our previous work. The proposed designs and development of the mechanical system of ResQbot 2.0, as well as the method for safely loading a full-body casualty onto the robot’s ‘stretcher bed’, are described in detail based on the conducted literature review, evaluation of our previous work, and feedback provided by medical professionals. We perform simulation experiments in the Gazebo physics engine simulator to verify the proposed design and the casualty extraction procedure. The simulation results demonstrate the capability of ResQbot 2.0 to carry out safe casualty extractions successfully.
Thermography as a Method to Detect Dental Anxiety in Oral Surgery
Authors: Gasparro Roberta , Leonetti Grazia , Riccio Michele , Irace Andrea , Sammartino Gilberto , Blasi Andrea , Scandurra Cristiano , Maldonato Nelson Mauro , Sammartino Pasquale , Marenzi Gaetano
Published: June 2021

(1) Background: the aim of this study was to evaluate if dental anxiety can be measured objectively using thermal infrared imaging. (2) Methods: Patients referred to the Department of Oral Surgery of the University of Naples Federico II and requiring dental extractions were consecutively enrolled in the study. Face thermal distribution images of the patients were acquired before and during their first clinical examination using infrared thermal cameras. The data were analyzed in relation to five regions of interest (ROI) of the patient’s face (nose, ear, forehead, zygoma, chin). The differences in the temperatures assessed between the two measurements for each ROI were evaluated by using paired T-test. The Pearson correlation and linear regression were performed to evaluate the association between differences in temperatures and Modified Dental Anxiety Scale (MDAS) questionnaire score, age, and gender; (3) results: sixty participants were enrolled in the study (28 males and 32 females; mean age 57.4 year-old; age range 18–80 year-old). Only for nose and ear zone there was a statistically significant difference between measurements at baseline and visit. Correlation between the thermal imaging measurements and the scores of the MDAS questionnaire was found for nose and ear, but not for all of the other regions. (4) Conclusions: the study demonstrated a potential use of thermal infrared imaging to measure dental anxiety.
Comparison of Satellite and Drone-Based Images at Two Spatial Scales to Evaluate Vegetation Regeneration after Post-Fire Treatments in a Mediterranean Forest
Authors: Martinez Jose Luis , Lucas-Borja Manuel Esteban , Plaza-Alvarez Pedro Antonio , Denisi Pietro , Moreno Miguel Angel , Hernández David , González-Romero Javier , Zema Demetrio Antonio
Published: June 2021

The evaluation of vegetation cover after post-fire treatments of burned lands is important for forest managers to restore soil quality and plant biodiversity in burned ecosystems. Unfortunately, this evaluation may be time consuming and expensive, requiring much fieldwork for surveys. The use of remote sensing, which makes these evaluation activities quicker and easier, have rarely been carried out in the Mediterranean forests, subjected to wildfire and post-fire stabilization techniques. To fill this gap, this study evaluates the feasibility of satellite (using LANDSAT8 images) and drone surveys to evaluate changes in vegetation cover and composition after wildfire and two hillslope stabilization treatments (log erosion barriers, LEBs, and contour-felled log debris, CFDs) in a forest of Central Eastern Spain. Surveys by drone were able to detect the variability of vegetation cover among burned and unburned areas through the Visible Atmospherically Resistant Index (VARI), but gave unrealistic results when the effectiveness of a post-fire treatment must be evaluated. LANDSAT8 images may be instead misleading to evaluate the changes in land cover after wildfire and post-fire treatments, due to the lack of correlation between VARI and vegetation cover. The spatial analysis has shown that: (i) the post-fire restoration strategy of landscape managers that have prioritized steeper slopes for treatments was successful; (ii) vegetation growth, at least in the experimental conditions, played a limited influence on soil surface conditions, since no significant increases in terrain roughness were detected in treated areas.
Optically Switchable MeV Ion/Electron Accelerator
Authors: Cohen Itamar , Gershuni Yonatan , Elkind Michal , Azouz Guy , Levanon Assaf , Pomerantz Ishay
Published: June 2021

The versatility of laser accelerators in generating particle beams of various types is often promoted as a key applicative advantage. These multiple types of particles, however, are generated on vastly different irradiation setups, so that switching from one type to another involves substantial mechanical changes. In this letter, we report on a laser-based accelerator that generates beams of either multi-MeV electrons or ions from the same thin-foil irradiation setup. Switching from generation of ions to electrons is achieved by introducing an auxiliary laser pulse, which pre-explodes the foil tens of ns before irradiation by the main pulse. We present an experimental characterization of the emitted beams in terms of energy, charge, divergence, and repeatability, and conclude with several examples of prospective applications for industry and research.
Size Effect on the Acoustic Emission Behavior of Textile-Reinforced Cement Composites
Authors: Ospitia Nicolas , Hardy Aymeric , Si Larbi Amir , Aggelis Dimitrios G. , Tsangouri Eleni
Published: June 2021

Acoustic emission (AE) is applied for the structural health evaluation of materials. It commonly uses piezoelectric sensors to detect elastic waves coming from energy releases within the material. Concerning cementitious composites as well as polymers, AE parameters have proven their potential to not only detect the existence of a defect, its location and the fracture mode, but also the developing strain field even before visible damage evolves. However, the wave propagation distance, wave dispersion due to plate geometry, heterogeneity and reflections result in attenuation and distortion of the AE waveforms. These factors render the interpretation more complex, especially for large samples. In this study, the effect of wave propagation on plain glass textile-reinforced cement (TRC) plates is investigated. Then, curved plates with different widths are mechanically loaded for bending with concurrent AE monitoring. The aim is to evaluate to what extent the plate dimensions and propagation distance influence the original AE characteristics corresponding to a certain fracture mechanism.
An Overview of Triggering Mechanisms and Characteristics of Local Strong Sandstorms in China and Haboobs
Authors: Gu Zhaolin , He Yuanping , Zhang Yunwei , Su Junwei , Zhang Renjian , Yu Chuck Wah , Zhang Daizhou
Published: June 2021

The local strong sandstorms (LSS), similar to haboobs in Sahara and the North America, often occur suddenly, in tens of minutes during the late afternoon, and before dusk in deserts in China, causing a significant impact on the local atmospheric environment. The Sudan haboob or American haboob often appears in the wet season, followed by thunderstorm events. In contrast, the LSS in China appears most frequently in relatively dry season. The lack of observational data in weather conditions before their formation, during their development and after their disappearance have hindered our understanding of the evolution mechanism of LSS/haboobs. This paper provides a review of the current status and model studies on LSS/haboobs in different time and space to characterize the weather conditions and triggering mechanisms for LSS/haboobs occurrence, as well as highlight the subject for further understanding of LSS/haboobs. LSS are always followed by the occurrence of a dry squall. The interaction of dust radiation heating in the near-surface mixing layer with a mesoscale anticyclone air mass (cold-air pool) in the upper layer is the key process that leads to an LSS. Haboobs are followed by the occurrence of a wet squall. The release of latent heat due to the condensation of water vapor, involving moist convection and cold downdraughts, is the main driving force that cause the occurrence of a haboob. For a better understanding of the characteristics of the wind-sand two-phase flow and the mechanism of energy dissipation in LSS/haboobs, further accumulation of meteorological observation data and small-scale multiple-phase numerical simulations are required.
2019–20 Australian Bushfires and Anomalies in Carbon Monoxide Surface and Column Measurements
Authors: John Shyno Susan , Deutscher Nicholas M. , Paton-Walsh Clare , Velazco Voltaire A. , Jones Nicholas B. , Griffith David W. T.
Published: June 2021

In Australia, bushfires are a natural part of the country’s landscape and essential for the regeneration of plant species; however, the 2019–20 bushfires were unprecedented in their extent and intensity. This paper is focused on the 2019–20 Australian bushfires and the resulting surface and column atmospheric carbon monoxide (CO) anomalies around Wollongong. Column CO data from the ground-based Total Carbon Column Observing Network (TCCON) and Network for the Detection of Atmospheric Composition Change (NDACC) site in Wollongong are used together with surface in situ measurements. A systematic comparison was performed between the surface in situ and column measurements of CO to better understand whether column measurements can be used as an estimate of the surface concentrations. If so, satellite column measurements of CO could be used to estimate the exposure of humans to CO and other fire-related pollutants. We find that the enhancements in the column measurements are not always significantly evident in the corresponding surface measurements. Topographical features play a key role in determining the surface exposures from column abundance especially in a coastal city like Wollongong. The topography at Wollongong, combined with meteorological effects, potentially exacerbates differences in the column and surface. Hence, satellite column amounts are unlikely to provide an accurate reflection of exposure at the ground during major events like the 2019–2020 bushfires.
Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production
Authors: Charria-Girón Esteban , Amazo Vanessa , De Angulo Daniela , Hidalgo Eliana , Villegas-Torres María Francisca , Baganz Frank , Caicedo Ortega Nelson. H.
Published: June 2021

Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of Chlorella sorokiniana was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth rate and yield. For instance, higher values of the specific growth rate (0.17 h−1) compared with those reported for the heterotrophic culture of Chlorella were obtained due to iron supplementation. Therefore, anaerobic sludge from an industrial wastewater treatment plant (a baker’s yeast company) was pretreated to obtain an extract as a media supplement for C. sorokiniana. According to the proposed model, the sludge extract allowed us to supplement iron values close to the growth activation concentration (KFe ~12 mg L−1). Therefore, a fed-batch strategy was evaluated on nitrogen-deprived cultures supplemented with the sludge extract to promote biomass formation and fatty acid synthesis. Our findings reveal that nitrogen and iron in sludge extract can supplement heterotrophic cultures of Chlorella and provide an alternative for the valorization of industrial anaerobic sludge.
Recovery of Bioactive Compounds from Industrial Exhausted Olive Pomace through Ultrasound-Assisted Extraction
Authors: Gómez-Cruz Irene , Contreras María del Mar , Carvalheiro Florbela , Duarte Luís C. , Roseiro Luisa B. , Romero Inmaculada , Castro Eulogio
Published: June 2021

Exhausted olive pomace (EOP) is the main agro-industrial waste of the olive pomace extracting industries. It contains phenolic compounds and mannitol, so the extraction of these bioactive compounds should be considered as a first valorization step, especially if EOP is used as biofuel. Therefore, EOP was subjected to bath-type ultrasound-assisted extraction (UAE), and the effects of the acetone concentration (20–80%, v/v), solid load (2–15%, w/v), and extraction time (10–60 min) on the extraction of antioxidant compounds were evaluated according to a Box–Behnken experimental design. By means of the response surface methodology, the optimum conditions were obtained: 40% acetone, 8.6% solids, and 43 min. For all the extracts, the total phenolic content (TPC), flavonoid content (TFC), and antioxidant activity (DPPH, ABTS, and FRAP) were determined. With the aim of shortening the extraction time, a two-level factorial experiment design was also carried out using a probe-type UAE, keeping the solid load at 8.6% (w/v) and the acetone concentration at 40% (v/v), while the amplitude (30–70%) and the extraction time (2–12 min) were varied to maximize the aforementioned parameters. Finally, a maximum of phenolic compounds was reached (45.41 mg GAE/g EOP) at 12 min and 70% amplitude. It was comparable to that value obtained in the ultrasonic bath (42.05 mg GAE/g EOP), but, remarkably, the extraction time was shortened, which translates into lower costs at industrial scale. Moreover, the bioactive compound hydroxytyrosol was found to be the major phenolic compound in the extract, i.e., 5.16 mg/g EOP (bath-type UAE) and 4.96 mg/g EOP (probe-type UAE). Other minor phenolic compounds could be detected by capillary zone electrophoresis and liquid-chromatography–mass spectrometry. The sugar alcohol mannitol, another bioactive compound, was also found in the extract, and its content was determined. Thus, the use of this technology can support the valorization of this waste to obtain bioactive compounds, including mannitol, hydroxytyrosol, and other derivatives, before being applied for other uses.
2La Paracentric Chromosomal Inversion and Overexpressed Metabolic Genes Enhance Thermotolerance and Pyrethroid Resistance in the Major Malaria Vector Anopheles gambiae
Authors: Ibrahim Sulaiman , Mukhtar Muhammad , Muhammad Abdullahi , Wondji Charles
Published: June 2021

Changes in global temperature are impacting the spread/intensity of vector-borne diseases, including malaria, and accelerating evolutionary/adaptive changes in vector species. These changes, including chromosomal inversions and overexpression and/or changes in allele frequencies of thermotolerance-associated genes, may facilitate insecticide resistance through pleiotropy. This study investigated the impact of thermotolerance on pyrethroid resistance in four populations of the malaria vector An. gambiae s.l., from the savanna/sub-Sahel of northern Nigeria. Anopheles coluzzii and An. gambiae s.s. were the only malaria vectors found, sympatric in all the sites, with the former species predominant. High thermotolerance was observed, with no mortality at 38 °C, and LT50 of ~44 °C. Significantly high permethrin resistance was observed (mortality < 50%) in 44 °C heat-hardened (exposure to an intermediately high temperature provides protection to a more severe temperature or insecticide) larvae from two sites, BUK and Pantami, compared with the control, and heat-hardened adult females from Auyo (mortality = 3.00% ± 1.20, χ2 = 5.83, p < 0.01) compared with the control (12.00% ± 4.65). The 2La chromosomal inversion was detected at ~50% in subset of larvae and 58% in subset of adult females genotyped. A significant association was observed (OR = 7.2, p < 0.03) between permethrin resistance and the 2La/+a rearrangement compared with 2L+a/+a, in BUK larvae. For all sites, permethrin resistance correlated with 2La/a homozygosity in adult females (R = 5.02, p = 0.01). qRT-PCR identified six genes commonly induced/overexpressed, including the heat shock protein 70 (AGAP004581) which was 2468× and 5× overexpressed in heat-hardened and permethrin-resistant females, respectively; trehalose-6-phosphate synthase (AGAP008227); and the ionotropic glutamate receptor genes, IR25a (AGAP010272) and IR21a (AGAP008511). This study highlights challenges associated with insecticide-based malaria vector control, and the epidemiological significance of taking climate variables into account for the design/choice of control measures.
Coumarin-Based Triapine Derivatives and Their Copper(II) Complexes: Synthesis, Cytotoxicity and mR2 RNR Inhibition Activity
Authors: Stepanenko Iryna , Babak Maria V. , Spengler Gabriella , Hammerstad Marta , Popovic-Bijelic Ana , Shova Sergiu , Büchel Gabriel E. , Darvasiova Denisa , Rapta Peter , Arion Vladimir B.
Published: June 2021

A series of thiosemicarbazone-coumarin hybrids (HL1-HL3 and H2L4) has been synthesised in 12 steps and used for the preparation of mono- and dinuclear copper(II) complexes, namely Cu(HL1)Cl2 (1), Cu(HL2)Cl2 (2), Cu(HL3)Cl2 (3) and Cu2(H2L4)Cl4 (4), isolated in hydrated or solvated forms. Both the organic hybrids and their copper(II) and dicopper(II) complexes were comprehensively characterised by analytical and spectroscopic techniques, i.e., elemental analysis, ESI mass spectrometry, 1D and 2D NMR, IR and UV–vis spectroscopies, cyclic voltammetry (CV) and spectroelectrochemistry (SEC). Re-crystallisation of 1 from methanol afforded single crystals of copper(II) complex with monoanionic ligand Cu(L1)Cl, which could be studied by single crystal X-ray diffraction (SC-XRD). The prepared copper(II) complexes and their metal-free ligands revealed antiproliferative activity against highly resistant cancer cell lines, including triple negative breast cancer cells MDA-MB-231, sensitive COLO-205 and multidrug resistant COLO-320 colorectal adenocarcinoma cell lines, as well as in healthy human lung fibroblasts MRC-5 and compared to those for triapine and doxorubicin. In addition, their ability to reduce the tyrosyl radical in mouse R2 protein of ribonucleotide reductase has been ascertained by EPR spectroscopy and the results were compared with those for triapine.
Bioinstructive Layer-by-Layer-Coated Customizable 3D Printed Perfusable Microchannels Embedded in Photocrosslinkable Hydrogels for Vascular Tissue Engineering
Authors: Sousa Cristiana F. V. , Saraiva Catarina A. , Correia Tiago R. , Pesqueira Tamagno , Patrício Sónia G. , Rial-Hermida Maria Isabel , Borges João , Mano João F.
Published: June 2021

The development of complex and large 3D vascularized tissue constructs remains the major goal of tissue engineering and regenerative medicine (TERM). To date, several strategies have been proposed to build functional and perfusable vascular networks in 3D tissue-engineered constructs to ensure the long-term cell survival and the functionality of the assembled tissues after implantation. However, none of them have been entirely successful in attaining a fully functional vascular network. Herein, we report an alternative approach to bioengineer 3D vascularized constructs by embedding bioinstructive 3D multilayered microchannels, developed by combining 3D printing with the layer-by-layer (LbL) assembly technology, in photopolymerizable hydrogels. Alginate (ALG) was chosen as the ink to produce customizable 3D sacrificial microstructures owing to its biocompatibility and structural similarity to the extracellular matrices of native tissues. ALG structures were further LbL coated with bioinstructive chitosan and arginine–glycine–aspartic acid-coupled ALG multilayers, embedded in shear-thinning photocrosslinkable xanthan gum hydrogels and exposed to a calcium-chelating solution to form perfusable multilayered microchannels, mimicking the biological barriers, such as the basement membrane, in which the endothelial cells were seeded, denoting an enhanced cell adhesion. The 3D constructs hold great promise for engineering a wide array of large-scale 3D vascularized tissue constructs for modular TERM strategies.
Diabetes Mellitus and Amyotrophic Lateral Sclerosis: A Systematic Review
Authors: Ferri Laura , Ajdinaj Paola , Rispoli Marianna Gabriella , Carrarini Claudia , Barbone Filomena , D’Ardes Damiano , Capasso Margherita , Muzio Antonio Di , Cipollone Francesco , Onofrj Marco , Bonanni Laura
Published: June 2021

Background: Amyotrophic Lateral Sclerosis (ALS) is a degenerative disorder which affects the motor neurons. Growing evidence suggests that ALS may impact the metabolic system, including the glucose metabolism. Several studies investigated the role of Diabetes Mellitus (DM) as risk and/or prognostic factor. However, a clear correlation between DM and ALS has not been defined. In this review, we focus on the role of DM in ALS, examining the different hypotheses on how perturbations of glucose metabolism may interact with the pathophysiology and the course of ALS. Methods: We undertook an independent PubMed literature search, using the following search terms: ((ALS) OR (Amyotrophic Lateral Sclerosis) OR (Motor Neuron Disease)) AND ((Diabetes) OR (Glucose Intolerance) OR (Hyperglycemia)). Review and original articles were considered. Results: DM appears not to affect ALS severity, progression, and survival. Contrasting data suggested a protective role of DM on the occurrence of ALS in elderly and an opposite effect in younger subjects. Conclusions: The actual clinical and pathophysiological correlation between DM and ALS is unclear. Large longitudinal prospective studies are needed. Achieving large sample sizes comparable to those of common complex diseases like DM is a challenge for a rare disease like ALS. Collaborative efforts could overcome this specific issue.
Cell-Based Therapies for Traumatic Brain Injury: Therapeutic Treatments and Clinical Trials
Authors: Bonilla Celia , Zurita Mercedes
Published: June 2021

Traumatic brain injury (TBI) represents physical damage to the brain tissue that induces transitory or permanent neurological disabilities. TBI contributes to 50% of all trauma deaths, with many enduring long-term consequences and significant medical and rehabilitation costs. There is currently no therapy to reverse the effects associated with TBI. An increasing amount of research has been undertaken regarding the use of different stem cells (SCs) to treat the consequences of brain damage. Neural stem cells (NSCs) (adult and embryonic) and mesenchymal stromal cells (MSCs) have shown efficacy in pre-clinical models of TBI and in their introduction to clinical research. The purpose of this review is to provide an overview of TBI and the state of clinical trials aimed at evaluating the use of stem cell-based therapies in TBI. The primary aim of these studies is to investigate the safety and efficacy of the use of SCs to treat this disease. Although an increasing number of studies are being carried out, few results are currently available. In addition, we present our research regarding the use of cell therapy in TBI. There is still a significant lack of understanding regarding the cell therapy mechanisms for the treatment of TBI. Thus, future studies are needed to evaluate the feasibility of the transplantation of SCs in TBI.
“Green” Prussian Blue Analogues as Peroxidase Mimetics for Amperometric Sensing and Biosensing
Authors: Gayda Galina Z. , Demkiv Olha M. , Gurianov Yanna , Serkiz Roman Ya. , Klepach Halyna M. , Gonchar Mykhailo V. , Nisnevitch Marina
Published: June 2021

Prussian blue analogs (PBAs) are well-known artificial enzymes with peroxidase (PO)-like activity. PBAs have a high potential for applications in scientific investigations, industry, ecology and medicine. Being stable and both catalytically and electrochemically active, PBAs are promising in the construction of biosensors and biofuel cells. The “green” synthesis of PO-like PBAs using oxido-reductase flavocytochrome b2 is described in this study. When immobilized on graphite electrodes (GEs), the obtained green-synthesized PBAs or hexacyanoferrates (gHCFs) of transition and noble metals produced amperometric signals in response to H2O2. HCFs of copper, iron, palladium and other metals were synthesized and characterized by structure, size, catalytic properties and electro-mediator activities. The gCuHCF, as the most effective PO mimetic with a flower-like micro/nano superstructure, was used as an H2O2-sensitive platform for the development of a glucose oxidase (GO)-based biosensor. The GO/gCuHCF/GE biosensor exhibited high sensitivity (710 A M−1m−2), a broad linear range and good selectivity when tested on real samples of fruit juices. We propose that the gCuHCF and other gHCFs synthesized via enzymes may be used as artificial POs in amperometric oxidase-based (bio)sensors.
Presence of the Endocannabinoid System in the Inferior Pulvinar of the Vervet Monkey
Authors: Micaelo-Fernandes Catarina , Bouskila Joseph , Bouchard Jean-François , Ptito Maurice
Published: June 2021

The expression of the endocannabinoid (eCB) system, including cannabinoid receptor type 1 (CB1R) and the cannabinoid synthesizing (NAPE-PLD) and degrading (FAAH) enzymes, has been well-characterized in the retina of rodents and monkeys. More recently, the presence of CB1R was localized throughout the dorsal lateral geniculate nucleus of the thalamus of vervet monkeys. Given that the retina projects also to the pulvinar either via a direct projection or via the superior colliculus, it was reasonable to assume that this system would be present therein. The visual pulvinar, namely the inferior pulvinar (PI) region, was delineated with calbindin immunohistochemical staining. Using Western blots and immunofluorescence, we demonstrated that CB1R, NAPE-PLD and FAAH are expressed in the PI of the vervet monkey. Throughout the PI, CB1R was mainly colocalized with VGLUT2-positive axon terminals in the vicinity of calbindin and parvalbumin-positive neurons. NAPE-PLD and FAAH rather colocalized with calbindin over the somatodendritic compartment of PI neurons. Our results suggest that visual information coming from the retina and entering the PI is modulated by the eCB system on its way to the dorsal visual stream. These results provide insights for understanding the role of eCBs in the modulation of visual thalamic inputs and, hence, visual perception.
Impact of Transcranial Direct Current Stimulation on Cognitive Function, Brain Functional Segregation, and Integration in Patients with Mild Cognitive Impairment According to Amyloid-Beta Deposition and APOE ε4-Allele: A Pilot Study
Authors: Kang Dong-Woo , Wang Sheng-Min , Kim Tae-Yeong , Kim Donghyeon , Na Hae-Ran , Kim Nak-Young , Lee Chang-Uk , Lim Hyun-Kook
Published: June 2021

Anodal transcranial direct current stimulation (anodal-tDCS) is known to improve cognition and normalize abnormal network configuration during resting-state functional magnetic resonance imaging (fMRI) in patients with mild cognitive impairment (MCI). We aimed to evaluate the impact of sequential anodal-tDCS on cognitive functions, functional segregation, and integration parameters in patients with MCI, according to high-risk factors for Alzheimer’s disease (AD): amyloid-beta (Aβ) deposition and APOE ε4-allele status. In 32 patients with MCI ([18 F] flutemetamol-: n = 10, [18 F] flutemetamol+: n = 22; APOE ε4-: n = 13, APOE ε4+: n = 19), we delivered anodal-tDCS (2 mA/day, five times/week, for 2 weeks) over the left dorsolateral prefrontal cortex and assessed the neuropsychological test battery and resting-state fMRI measurements before and after 2 weeks stimulation. We observed a non-significant impact of an anodal-tDCS on changes in neuropsychological battery scores between MCI patients with and without high-risk factors of AD, Aβ retention and APOE ε4-allele. However, there was a significant difference in brain functional segregation and integration parameters between MCI patients with and without AD high-risk factors. We also found a significant effect of tDCS-by-APOE ε4-allele interaction on changes in the functional segregation parameter of the temporal pole. In addition, baseline Aβ deposition significantly associated negatively with change in global functional integrity of hippocampal formation. Anodal-tDCS might help to enhance restorative and compensatory intrinsic functional changes in MCI patients, modulated by the presence of Aβ retention and the APOE ε4-allele.
Navigating in Virtual Environments: Does a Map or a Map-Based Description Presented Beforehand Help?
Authors: Meneghetti Chiara , Pazzaglia Francesca
Published: June 2021

Background. One of the aims of research in spatial cognition is to examine the factors capable of optimizing environment learning from navigation, which can be examined using a virtual environment (VE). Different learning conditions can play an important part. Aim. This study examined the benefits of presenting configured information (layout with elements arranged in it) using a map or verbal description before a learner navigates in a new environment. Method. Ninety participants were assigned to three learning groups of 30 individuals (15 males and 15 females). Before participants navigated in a VE, one group was shown a map of the environment (“map before navigation”), a second group read a map-like description of the environment (“description before navigation”), and a third group started navigating without any prior input (“only navigation”). Participants then learned a path in a VE (presented as if they were driving a car). Their recall was subsequently tested using three types of task: (i) route retracing; (ii) pointing; (iii) path drawing. Several measures were administered to assess participants’ individual visuospatial and verbal factors. Results. There were no differences between the three groups in route retracing. The “map before navigation” group performed better than the “only navigation” group in both the pointing and the path drawing tasks, however, and also outperformed the “description before navigation” group in the path drawing task. Some relations emerged between participants’ individual difference factors and their recall performance. Conclusions. In learning from navigation, seeing a map beforehand benefits learning accuracy. Recall performance is also supported, at least in part, by individual visuospatial and verbal factors.
Sitting in Judgment: How Body Posture Influences Deception Detection and Gazing Behavior
Authors: Zloteanu Mircea , Krumhuber Eva G. , Richardson Daniel C.
Published: June 2021

Body postures can affect how we process and attend to information. Here, a novel effect of adopting an open or closed posture on the ability to detect deception was investigated. It was hypothesized that the posture adopted by judges would affect their social acuity, resulting in differences in the detection of nonverbal behavior (i.e., microexpression recognition) and the discrimination of deceptive and truthful statements. In Study 1, adopting an open posture produced higher accuracy for detecting naturalistic lies, but no difference was observed in the recognition of brief facial expressions as compared to adopting a closed posture; trait empathy was found to have an additive effect on posture, with more empathic judges having higher deception detection scores. In Study 2, with the use of an eye-tracker, posture effects on gazing behavior when judging both low-stakes and high-stakes lies were measured. Sitting in an open posture reduced judges’ average dwell times looking at senders, and in particular, the amount and length of time they focused on their hands. The findings suggest that simply shifting posture can impact judges’ attention to visual information and veracity judgments (Mg = 0.40, 95% CI (0.03, 0.78)).
Strategies for Adopting Building Information Modeling (BIM) in Sustainable Building Projects—A Case of Malaysia
Authors: Manzoor Bilal , Othman Idris , Gardezi Syed Shujaa Safdar , Harirchian Ehsan
Published: June 2021

In terms of sustainable growth, Malaysia’s construction industry faces significant challenges, such as construction costs and lack of awareness. To meet these challenges, it is essential to integrate building information modeling (BIM) and sustainable development. As a result, this study aimed to establish strategies for adopting BIM in sustainable building projects. A systematic literature review was performed to classify the strategies to accomplish this aim, followed by a questionnaire survey of 129 construction project stakeholders. Mean score and exploratory factor analysis (EFA) were conducted to explore the detailed influence of BIM adoption strategies in sustainable building projects. The results revealed that “workshops, lectures, and conference events are used to enhance public awareness” and “better information on the costs and benefits of sustainable materials” and “strengthened sustainable development” were the most essential strategies to boost sustainable growth in building projects. Furthermore, EFA was conducted to undertake the group analysis, namely, to identify the standardization-related strategy, economic-related strategy, awareness-related strategy, and environment-related strategy. This study paves the way for future scholars, policymakers, and practitioners to investigate the limitations of future studies.
The Improvement of Indoor Air Quality in Residential Buildings in Dubai, UAE
Authors: Jung Chuloh , Awad Jihad
Published: June 2021

Due to unprecedented urbanization, UAE had built many new residential projects with poor choices of material and ventilation. This social phenomenon is leading UAE to Sick Building Syndrome (SBS) faster than any other countries. The Dubai Municipality regulates the indoor air quality with strict stipulation, but the detailed regulations are still insufficient. The objective of this paper is to measure the indoor air quality of new residential projects in Dubai to suggest the improvement of the regulations for indoor air quality. As a methodology, a field survey was conducted to investigate the status of indoor air pollution in residential buildings. Based on the field survey data, lab experiments for building materials were conducted and a computer simulation on radon gas was conducted. The result had shown that radon gas was mainly detected in new townhouses and labor camp houses, and its concentration was found to exceed the standard. Volatile organic compounds (VOCs) and formaldehyde (CH2O) were mainly detected in showhouses and new townhouses, and the concentration distribution was about 10 times higher than that of outdoors. It was proven that emission concentration of radon gas from various building materials were detected, and the order was red clay, gypsum board, and concrete. Volatile organic compounds (VOCs) are mainly detected in oil paints and PVC floor and the radiation amount of all pollutants increased with temperature increase. In computer simulation, it was found that a new townhouse needs a grace period from 20 days to 6 months to lower the radon gas concentration by 2 pCi/L. This study will serve as a basic data to establish more detailed regulation for the building materials and improve the IAQ standards in Dubai.
Is the Concurrent Use of Sorafenib and External Radiotherapy Feasible for Advanced Hepatocellular Carcinoma? A Meta-Analysis
Authors: Rim Chai Hong , Park Sunmin , Shin In-Soo , Yoon Won Sup
Published: June 2021

We evaluate the feasibility of a concurrent application of sorafenib and external beam radiation therapy (EBRT) for advanced hepatocellular carcinoma (HCC). PubMed, Embase, Medline, and Cochrane Library were searched up to 9 April 2021. The primary endpoint was grade ≥3 complications, and the secondary endpoint was overall survival (OS). Subgroup analyses were performed for studies with the EBRT targets, intrahepatic vs. non-intrahepatic lesions (e.g., extrahepatic metastases or malignant vessel involvement only). Eleven studies involving 512 patients were included in this meta-analysis. Pooled rates of gastrointestinal, hepatologic, hematologic, and dermatologic grade ≥3 toxicities were 8.1% (95% confidence interval (CI): 4.8–13.5, I2 = ~0%), 12.9% (95% CI: 7.1–22.1, I2 = 22.4%), 9.1% (95% CI: 3.8–20.3, I2 = 51.3%), and 6.8% (95% CI: 3.8–11.7, I2 = ~0%), respectively. Pooled grade ≥3 hepatologic and hematologic toxicity rates were lower in studies targeting non-intrahepatic lesions than those targeting intrahepatic lesions (hepatologic: 3.3% vs. 17.1%, p = 0.041; hematologic: 3.3% vs. 16.0%, p = 0.078). Gastrointestinal and dermatologic grade ≥3 complications were not significantly different between the subgroups. Regarding OS, concurrent treatment was more beneficial than non-concurrent treatment (odds ratio: 3.3, 95% CI: 1.3–8.59, p = 0.015). One study reported a case of lethal toxicity due to tumor rupture and gastrointestinal bleeding. Concurrent treatment can be considered and applied to target metastatic lesions or local vessel involvement. Intrahepatic lesions should be treated cautiously by considering the target size and hepatic reserve.
Identification of Lung and Blood Microbiota Implicated in COVID-19 Prognosis
Authors: Dereschuk Kypros , Apostol Lauren , Ranjan Ishan , Chakladar Jaideep , Li Wei Tse , Rajasekaran Mahadevan , Chang Eric Y. , Ongkeko Weg M.
Published: June 2021

The implications of the microbiome on Coronavirus disease 2019 (COVID-19) prognosis has not been thoroughly studied. In this study we aimed to characterize the lung and blood microbiome and their implication on COVID-19 prognosis through analysis of peripheral blood mononuclear cell (PBMC) samples, lung biopsy samples, and bronchoalveolar lavage fluid (BALF) samples. In all three tissue types, we found panels of microbes differentially abundant between COVID-19 and normal samples correlated to immune dysregulation and upregulation of inflammatory pathways, including key cytokine pathways such as interleukin (IL)-2, 3, 5-10 and 23 signaling pathways and downregulation of anti-inflammatory pathways including IL-4 signaling. In the PBMC samples, six microbes were correlated with worse COVID-19 severity, and one microbe was correlated with improved COVID-19 severity. Collectively, our findings contribute to the understanding of the human microbiome and suggest interplay between our identified microbes and key inflammatory pathways which may be leveraged in the development of immune therapies for treating COVID-19 patients.
Future Perspectives of Therapeutic, Diagnostic and Prognostic Aptamers in Eye Pathological Angiogenesis
Authors: Iturriaga-Goyon Emilio , Buentello-Volante Beatriz , Magaña-Guerrero Fátima Sofía , Garfias Yonathan
Published: June 2021

Aptamers are single-stranded DNA or RNA oligonucleotides that are currently used in clinical trials due to their selectivity and specificity to bind small molecules such as proteins, peptides, viral particles, vitamins, metal ions and even whole cells. Aptamers are highly specific to their targets, they are smaller than antibodies and fragment antibodies, they can be easily conjugated to multiple surfaces and ions and controllable post-production modifications can be performed. Aptamers have been therapeutically used for age-related macular degeneration, cancer, thrombosis and inflammatory diseases. The aim of this review is to highlight the therapeutic, diagnostic and prognostic possibilities associated with aptamers, focusing on eye pathological angiogenesis.