bluuebunny/crossref_metadata_2025 huggingface dataset:
Jaringan Nilai dan Sikap Konsumen terhadap Kualitas dan Private Label pada Komoditas Brokoli Organik di Ritel Modern (Suatu Kasus di Lotte Mart Festival Citylink Bandung, Jawa Barat)
Authors: Risdianto Ari , Utami Hesty Nurul , Mukti Gema Wibawa
Published: February 2020

Pemasaran produk tercipta karena adanya hubungan kerjasama antar pelaku pemasaran. Salah satunya antara supplier dengan ritel modern. Hubungan kerjasama membentuk jaringan nilai bagi supplier dengan ritel modern untuk menjadi titik kunci membangun hubungan jangka panjang untuk terus meningkatkan kualitas produk dan jasa. Terutama ritel modern yang berkembang dengan pesat memiliki harapan mendapatkan keuntungan yang lebih dan memberikan image lebih kepada konsumen dengan meningkatkan kualitas produk dan penggunaan private label. Pada akhirnya, sikap konsumen yang mampu mengevaluasi seluruh atribut produk sesuai dengan kebutuhannya. Penelitian ini bertujuan untuk mengetahui penerapan kualitas brokoli organik dan penggunaan private label pada brokoli organik dalam jaringan nilai antara supplier dengan ritel modern dengan menggunakan Value Network Mapping yang dikaitkan dengan sikap konsumen dalam menilai kualitas dan private label yang diterapkan menggunakan model multiatribut Fishbein. Penelitian dilaksanakan di Lotte Mart Festival City Link Bandung. Desain penelitian adalah kuantitatif dan kualitatif. Hasil dari analisis jaringan nilai adalah kerjasama yang dilakukan oleh Lotte Mart dengan Bimandiri sudah berjalan baik kurang lebih 6 tahun. Persepsi antara kedua belah pihak sudah tercipta dengan baik mulai dari penerapan kualitas brokoli organik dan label L Choice dan Everydayfresh. Hasil dari analisis multiatribut Fishbein, 14 atribut brokoli yang diuji yaitu ukuran, bentuk, warna, kemulusan, kesegaran, kerenyahan, daya tahan, cita rasa, kandungan nutrisi, keamanan produk, harga jual, presentasi, promosi dan kemenarikan kemasan. Terdapat 3 nilai atribut tertinggi yang telah sesuai dengan harapan pada tingkat evaluasi konsumen brokoli organik yaitu kemulusan, warna dan keamanan produk sedangkan pada brokoli non organik yaitu kesegaran, kerenyahan dan harga jual.
EKUITAS MEREK PENGARUHNYA TERHADAP KEPUTUSAN PEMBELIAN XL PAKET DATA SELULER DI KABUPATEN BANGKALAN
Authors: Muidz Abdul , Wahyuningsih Diana , Roosmawan Hery , Wuryanti Henny
Published: February 2020

Merek sangatlah penting karena konsumen tidak lagi puas hanya dengan ter-cukupi kebutuhannya. Besarnya pengaruh yang diberikan oleh suatu merek yang sudah dianggap sebagai aset perusahaan (brand equity) maka PT. XL Axiata menginginkan mereknya sukses di pasaran. Tujuan dari penelitian ini adalah untuk mengetahui Ekuitas Merek yang dimiliki oleh PT. XL Axiata sehingga dapat mempengaruhi konsumen pada keputusan pembelian XL Paket Data Seluler di Kabupaten Bangkalan. Penelitian menggunakan metode penelitian asosiatif. Tehnik analisis menggunakan regresi linear berganda, mengingat model tersusun atas sejumlah hubungan kausal antar variabel laten. Hasil penelitian menunjukkan secara simultan dan parsial kesadaran merek, asosiasi merek, persepsi kualitas, dan loyalitas merek berpengaruh positif dan signifikan terhadap keputusan pembelian konsumen. Manajemen PT. XL Axiata sebaiknya meningkatkan kesadaran merek, asosiasi merek, persepsi kualitas, dan loyalitas merek bagi para konsumennya, mengingat koefisien regresinya masih rendah atau masih dibawah satu. Hal ini dapat dilakukan dengan cara melakukan peningkatan kualitas produk dan layanan gerai bagi para konsumen serta mengadakan kegiatan-kegiatan marketing untuk meningkatkan brand awarness konsumennya.
Analisis Informasi Laporan Arus Kas Untuk Mengukur Kinerja Keuangan Pada Pt Semen Indonesia (Persero) Tbk Periode 2014-2016 Yang Terdaftar Di Bursa Efek Indonesia
Authors: Sakti Hamidah Aqnes Dwi
Published: February 2020

PT. Semen Indonesia (Persero) Tbk reports cash flow reports annually to make use of cash flow report  information  as  an important company  performance  analysis tool.  One analysis of financial performance using cash flow statements is the ratio of cash flow statements. The purpose of this study is to find out how to analyze cash flow statements to measure financial performance at PT. Semen Indonesia (Persero) Tbk from 2014 to 2016. This study uses data analysis techniques with qualitative descriptive methods, namely by calculating the ratio of operating cash flows, the ratio of cash coverage to current debt, the ratio of capital expenditure, the ratio of total debt, the ratio of cash to interest coverage , and the ratio of the flow of funds. The results of this study indicate that financial performance at PT. Semen Indonesia (Persero) Tbk in 2014 to 2016 is where from six ratios are calculated into two ratios that meet the standards of more than one, namely the CKB and CAD ratios and the other four ratios are below one. Means that all cash flows owned by the company only have more potential to pay short- term liabilities.
Peranan Career Anchor dalam Menciptakan Kepuasan Kerja Pegawai Dinas Kesehatan Kabupaten Sampang
Authors: Wispandono Moch.
Published: February 2020

Career  Anchor  dideskripsikan  sebagai  sebuah  elemen  dalam  konsep  diri  seseorang(berhubungan dengan karir) yang tidak akan mereka lepaskan atau korbankan, bahkan pada saat menghadapi kondisi sulit. Oleh karena itulah setiap karyawan akan berusaha untuk dapat menduduki posisi jabatan tertentu yang lebih layak dan semakin meningkat dari waktu ke waktu sehingga kepuasan dalam bekerja akan muncul. Uraian di atas menjadi  alasan  untuk  dilakukan  penelitian  ini,  dengan  berupaya  untuk  menjawab masalah  yang dirumuskan  sebagai  berikut:  (1)  Apakah  faktor  Career  Anchor  yang terdiri dari Managerial Competence (kompetensi manajerial), Technical/ functional Competence (kompetensi teknis/ fungsional), Security/ stability (keamanan/ keseimbangan), Creativity (kreativitas) dan Autonomy (kebebasan) secara simultan berpengaruh terhadap kepuasan kerja karyawan Dinas Kesehatan Pemda Kabupaten Sampang? (2) Apakah variabel kompetensi teknis/ fungsional dalam Career Anchor di atas mempunyai pengaruh dominan terhadap kepuasan kerja karyawan Dinas Kesehatan Pemda  Kabupaten  Sampang.  Berdasarkan  pada  tujuan  penelitian,  hipotesis  dan kerangka konseptual. dapat ditarik beberapa kesimpulan sebagai berikut: (1) Hasil perhitungan SPSS menunjukkan nilai Fhitung= 8.349. Hal ini berarti bahwa secara simultan variabel Career Anchor (X) mempunyai pengaruh terhadap kepuasan kerja karyawan (Y). Hal ini menunjukkan bahwa secara bersama-sama variable yang terdapat dalam Career Anchor berpengaruh  signifikan terhadap kepuasan kerja karyawan Dinas Kesehatan Kabupaten Sampang. (2) Variabel  kompetensi teknis berpengaruh dominan terhadap Kepuasan Kerja Karyawan Dinas Kesehatan Kabupaten Sampang karena nilai t hitung variabel ini paling besar daripada variable bebas lainnya dengan nilai 2.889 yang lebih besar dari ttabel (2.889 1.985).
Etika Perencanaan Pariwisata (Studi Pengembangan Pariwisata Pulau Gili Labak Sumenep Jawa Timur)
Authors: Resdiana Enza , Widyastuti Endang
Published: February 2020

Penelitian ini dilakukan untuk mengetahui bagaimana etika perencanaan pariwisata pada pengembangan pulau Gili Labak Sumenep, Jawa Timur. Penelitian ini menggunakan metode kualitatif deskriptif, adapun pengumpulan data dengan menggunakan pengamatan langsung (observasi) dan wawancara mendalam (in-depth interview). Hasil penelitian menunjukkan bahwa prospek pasar belum terpetakan sesuai keinginan pasar karena keterbatasan sarpras wisata, analisa situasi berkaitan dengan faktor ekologi, sosial dan lingkungan belum terdapat pengelolaan yang baik karena justru menimbulkan efek eksternalitas terutama sampah pengujung yang mengakibatkan pencemaran, produk pariwisata yang dapat memuaskan wisatawan masih terbatas pada suguhan alam yang belum didukung produk riil seperti cinderamata khas sebagai kenang-kenangan wisatawan, promosi terbatas pada media sosial, biro perjalanan wisata pun masih terbatas pada trip/ rute perjalanan yang tidak setiap saat, minim publikasi, workshop. Pemberdayaan yang setempat sudah melibatkan CSR perusahaan khususnya Madura Offshore, hal ini sangat membantu sarpras seperti bubu rajungan, energi panas matahari, gazebo wisata, neonbox termasuk pemberdayaan untuk memunculkan bahwa masyarakat lokal mempunyai peran strategis untuk keberlangsungan wisata pulau gili labak. Namun perencanaan pariwisata dalam pengembangannya masih menghadapi banyak kendala, seperti fasilitas transportasi dari sumenep darat menuju pulau, penginapan, kebutuhan air tawar, energi listrik, pemasaran/promosi wisata, target pasar, pemberdayaan kepada masyarakat setempat, analisa situasi untuk keberlanjutan wisata yang tentu saja membutuhkan perencanaan dan dukungan pemerintah, swasta dan masyarakat Sumenep khususnya.
Niveles de conservación y degradación de hábitat en los ecosistemas de una cuenca de alta biodiversidad en Tamaulipas (México)
Authors: Requena Lara Glenda Nelly , Morales Pacheco Juan Francisco , Cámara Artigas Rafael , Zamora Tovar Carlos
Published: February 2020

Los ecosistemas con flujos de servicios ambientales óptimos ofrecen una adecuada calidad en sus hábitats que coadyuvan a mantener su biodiversidad. Esta calidad puede reducirse por los factores de mayor intensidad y cercanía al hábitat, lo que puede estudiarse con herramientas geoespaciales. Este trabajo evaluó los niveles de degradación relativa de hábitat en la cuenca Guayalejo-Tamesí (Tamaulipas, México), respecto a los factores adyacentes que amenazan su calidad, usando el modelador Habitat Quality del Toolbox InVest 1.005beta para ArcGis-9.2. Los ecosistemas con menor amenaza a su hábitat (degradación nula o menor a 20 %) y con mejores oportunidades de conservación, representan el 77,3 % de la superficie de la cuenca. Particularmente, los hábitats montañosos (bosques templados y mesófilo) son los mejor conservados, pues más del  80 % de su superficie se encuentra libre de degradación; destaca el bosque mesófilo de montaña, con un  94 % de su superficie no degradada, debido a su escasa exposición a factores amenazantes. Se concluye que, a pesar del uso intensivo del territorio de la cuenca, existen ecosistemas bien conservados, especialmente en zonas aisladas; la mayoría de ellas carecen de protección legal, por lo que se encuentran expuestas a la ampliación y agravamiento de las amenazas antropogénicas estudiadas.
Medication Burden And Inappropriate Prescription Risk Among Elderly With Advanced Chronic Kidney Disease
Authors: Roux-Marson Clarisse , Baranski Jean-Baptiste , Fafin Coraline , Extermann Guillaume , Vigneau Cecile , Couchoud Cecile , Moranne Olivier
Published: February 2020

Background Elderly patients with chronic kidney disease (CKD) frequently present comorbidities that put them at risk of polypharmacy and medication-related problems. This study aims to describe the overall medication profile of patients aged ≥ 75 years with advanced CKD from a multicenter French study and specifically the renally (RIMs) and potentially inappropriate-for-the-elderly medications (PIMs) that they take. Methods This is a cross-sectional analysis of medication profiles of individuals aged ≥ 75 years with eGFR < 20 ml/min/1.73m2 followed by a nephrologist, who collected their active prescriptions at the study inclusion visit. Medication profiles were first analyzed according to route of administration, therapeutic classification. Second, patients were classified according to their risk of potential medication-related problems, based on whether the prescription was a RIM or a PIM. RIMs and PIMs have been defined according to renal appropriateness guidelines and to Beer’s criteria in the elderly. RIMs were subclassified by 4 types of category : (a) contraindication; (b) dose modification is recommended based on creatinine clearance (CrCl); (c) dose modification based on CrCl is not recommended but a maximum daily dose is mentioned, (d) no specific recommendations based on CrCl : “use with caution”, “avoid in severe impairment”, “careful monitoring of dose is required” “reduce the dose”. Results We collected 5196 individual medication prescriptions for 556 patients, for a median of 9 daily medications [7-11]. Antihypertensive agents, antithrombotics, and antianemics were the classes most frequently prescribed. Moreover, 77.0% of patients had at least 1 medication classified as a RIM. They accounted 31.3% of the drugs prescribed and 9.25% was contraindicated drugs. At least 1 PIM was taken by 57.6% and 45.5% of patients had at least one medication classified as RIM and PIM. The prescriptions most frequently requiring reassessment due to potential adverse effects were for proton pump inhibitors and allopurinol. The PIMs for which deprescription is especially important in this population are rilmenidine, long-term benzodiazepines, and anticholinergic drugs such as hydroxyzine. Conclusion We showed potential drug-related problems in elderly patients with advanced CKD. Healthcare providers must reassess each medication prescribed for this population, particularly the specific medications identified here.
Maternal and partner’s level of satisfaction on the delivery room service in University of Gondar Referral Hospital, Northwest, Ethiopia: A comparative cross-sectional study.
Authors: Taddese Asefa Adimasu , Gashaye Kiros Terefe , Dagne Henok , Andualem Zewudu
Published: February 2020

Background: Asking patients/clients what they perceive about the care and treatment they have received is one of the important steps towards improving the quality of health care. In the scientific world, a number of efforts have been tried to understand about what laboring mothers perceive about the care provided. However, little is known about the birth experiences of partners in Ethiopia. Therefore, this study was aimed to assess the maternal and partner’s level of satisfaction on the delivery room service in the study area.
Methods: A comparative cross-sectional study was conducted from Dec 2018 to January 2019 in University of Gondar referral hospital. The birth satisfaction scale is used for the mother, and it was adapted to the partners’ perspective. Paired-samples t tests were used for comparing mothers and partners for the birth satisfaction scales global and thematic scores. A binary logistic regression model was fitted to identify predicting factors for mothers’ and partners satisfaction.
Results: The overall satisfaction of mothers in this study was 47.6 % whereas 41.2% of partners were satisfied by delivery room services. There were mean difference between mothers and partners’ birth satisfaction scale (p=0.02). Maternal satisfaction scale was affected by age [OR = 0.36, 95%CI (0.18, 0.73 )], perception [OR =0.02, 95%CI (0.001,0.09)], waiting time [OR = 0.11 , 95%CI (0.001, 0.09)],visiting mode [OR = 0.01 , 95%CI (0.001,0.08)], pregnancy status [OR = 0.04, 95%CI (0.01 ,0.33)] and fatal outcome [OR=0.001, 95%CI (0.001,0.018)] .whereas, partners satisfaction was associated with age [OR=0.16,95%CI (0.05 0.49)], occupational status [OR = 0.02, 95%CI (0.001, 0.24), amount of money to pay for service [OR = 2.87, 95%CI (1.07, 7.71), visiting mode of his wife [OR = 0.08, 95%CI (0.01, 0.35)], waiting time [OR = 0.12, 95%CI (0.04, 0.33)], privacy [OR = 10.61, 95%CI (3.00, 37.52)], mode of delivery of his wife [OR = 7.69, 95%CI (3.00, 19.69)].
Conclusion : This finding would alert the health care system to design a client-friendly approach. It will provide insight to hospital administrators and providers in formulating a policy that would enhance the support of partners during labour and delivery process.
Effect of prenatal counseling on breastfeeding self-efficacy and frequency of breastfeeding problems in Mothers with Previous Unsuccessful Breastfeeding: A randomized controlled clinical trial
Authors: shafaei fahimeh sehhatie , mirghafourvand mojgan , havizari shiva
Published: February 2020

Background: Breastfeeding is one of the most important interfering factors in infants’ health. Monitoring mothers’ performance and providing them with the feedback helps to increase their self-efficacy, interest in learning, and level of performance. The present research evaluates the effect of prenatal counseling on the breastfeeding self-efficacy and frequency of breastfeeding problems in mothers with previous unsuccessful breastfeeding. Methods: This randomized controlled clinical trial was conducted on 108 pregnant women with unsuccessful breastfeeding in Tabriz health centers during 2017-2018. The participants were randomly assigned to intervention and control groups. The intervention group had four prenatal counseling sessions and the controls only received routine care. Then, the mothers who gave birth to their children received a counseling session up to 4 months after the delivery. The Breastfeeding Self-Efficacy (BSES) questionnaire and the frequency of breast feeding problems checklist on the 15 th day, and 2 nd and 4 th month were completed both by the intervention and control groups. Results: The mean (SD) of breastfeeding self-efficacy was 119.3 (10.5), 128.3 (8.3) and 133.8 (10.3) in the intervention group and 105.3 (16.1), 105.7 (19.7) and 109.4 (24.7) in the control group on the 15 th day, 2 nd and 4 th month after the delivery, respectively. There was a significant difference in terms of breastfeeding self-efficacy between intervention and control group on the 15 th day (p<0.001), and 2 nd (p<0.001) and 4 th (p<0.001) month after the delivery. The frequency of breastfeeding problems on the 15 th (p=0.008), 2 nd (p<0.001) and 4 th (p<0.001) after the delivery was significantly different in most cases of the intervention group when compared to the controls. Conclusion : The results indicated that prenatal counseling can increase mothers’ breastfeeding self-efficacy and solves most breastfeeding problems during postpartum period. Trial registration: IRCT20100109003027N19 Key words: Self-efficacy, problems, exclusive breastfeeding, infant, counseling
Stress-related coping styles in myalgic university students: A case control study.
Authors: Soto-Goñi Xabier , Viñals Ana Cristina , Pérez-González Fabian , Sánchez-Labrador Luis , Domínguez-Gordillo Adelaida , Sánchez-Sánchez Teresa , Ardizone-García Ignacio , Jiménez-Ortega Laura
Published: February 2020

Background: Patients suffering pain related temporomandibular disorders (TMD) exhibit greater levels of psychological distress, environmental stress, somatic symptoms, anxiety, depression, somatic awareness, pain catastrophizing, and impaired pain coping strategies compared to pain-free controls. However, little is known about psychological factors involved in the different TMD types fulfilling DC/TMD criteria. Furthermore, regardless of severity, the role of general coping strategies and styles in TMD is not yet well understood. The main goal of this study was to investigate stress-related coping styles, anxiety and personality traits in a group of dentistry students suffering from temporomandibular disorder with myalgia.
Methods: A cohort of 102 university students was initially recruited for this study. Following clinical evaluation, a myalgia group (24 participants) and a control group (25 participants) were formed. Participants were later assessed in anxiety, stress coping strategies, and personality measures by using the State-trait anxiety inventory (STAI), coping response inventory (CRI), and Neo Five-Factor Inventory (NEO-FFI) questionnaires respectively.
Results: The myalgia group presented greater levels of trait anxiety and neuroticism in comparison to the control group. In addition, participants with myalgia showed higher levels of avoidance coping.
Conclusions: Avoidance coping strategies are generally considered maladaptive, as they seem to increase perceived stress, a robust predictor of TMD. Interventions aimed at reducing stress levels and preventing maladaptive coping styles, might improve temporomandibular health and prevent the myalgia and its chronification.
Identification and analysis of KLF13 variants in patients with congenital heart disease
Authors: Li Wenjuan , Li Baolei , Li Tingting , Zhang Ergeng , Wang Qingjie , Chen Sun , Kun Sun
Published: February 2020

Background: The protein Kruppel-like factor 13 (KLF13) is a member of the KLF family that has been identified as a cardiac transcription factor which is involved in heart development. However, the relationship between KLF13 variants and CHDs in humans remains largely unknown. The present study aimed to screen the KLF13 variants in CHDs patients and genetically analyze the function of these variants. Methods: KLF13 variants were sequenced in a cohort of 309 CHD patients and population-matched healthy controls (n = 200) using targeted sequencing. To investigate the effect of variants on the functional ability of the KLF13 protein, the expressions and subcellular localization of protein, as well as the transcriptional activities of downstream genes and physically interacted with other transcription factor were assessed. Results: Two heterozygous variants, c.487C>T (P163S) and c.467G>A (S156N), were identified in two out of 309 CHDs patients with Tricuspid-valve atresia and transposition of the great arteries, respectively. No variants were found among healthy controls. The variant c.467G>A (S156N) increased protein expression and enhanced functionality compared with that of wild-type, without affecting the subcellular localization. The other variant, c.487C>T (P163S), did not show any abnormalities in protein expression and subcellular localization, however it eliminated the transcriptional activities of downstream target genes and physically interacted with TBX5, another cardiac transcription factor. Conclusion: Our results show that the S156N and P163S variants may affect the transcriptional function of KLF13 and physical interaction with TBX5, These results identified KLF13 maybe as a potential genetic risk factor for congenital heart disease.
Distribution of malaria patients seeking care in different types of health facilities during the implementation of National Malaria Elimination Program
Authors: Li Gang , Zhang Donglan , Chen Zhuo , Feng Da , Chen Xiaoyu , Tang Shangfeng , Son Heejung , Wang Zhenhua , Xi Yuanhang , Feng Zhanchun
Published: February 2020

Background: China launched the National Malaria Elimination Program (NMEP) in 2010 that set the goal that all health facilities had the ability to diagnose malaria along with the hospitals at all levels had the ability to treat malaria by 2015. To provide a reference for the control of imported malaria, we conducted a study on the distribution of malaria patients seeking care in different types of health facilities.

Methods: The data used for the analysis was obtained through the Infectious Diseases Information Reporting Management System (IDIRMS) and stratified multistage sampling. Descriptive statistical analysis was used to investigate the distribution of malaria patients in different types of health facilities, and the Chi-square test was used to compare the proportions of patients seeking care outside their current residence region between different types of hospitals. Point maps were drawn to visualize the spatial distribution of hospitals reporting malaria cases, and flow maps were created to show spatial flow of malaria patients by using the ArcGIS software.

Results: The proportions of malaria patients who sought care in hospitals, township hospitals, and Centers for Disease Control and Prevention (CDCs) were 81.7%, 14.7% and 3.6%, respectively. For those who sought care in hospitals, the percentages who sought care in provincial, prefecture-level and county-level hospitals were 17.4%, 60.5% and 22.1%, correspondingly; the proportions of malaria patients who sought care in tertiary hospitals, secondary hospitals, and primary hospitals were 59.8%, 39.9% and 0.3%, respectively. Moreover, the proportions of patients seeking care in hospitals within county and prefectural administrative area were 18.2%, 63.4%, respectively.

Conclusion: During the implementation of NMEP, malaria patients tended to seek care in tertiary hospitals and prefecture-level hospitals, and more than half of patients could be treated in hospitals in prefecture-level areas. In the current phase, it is necessary to establish referral system from grassroots medical institutions to hospitals designated for malaria treatment.

Keywords: Geographic variation; Care-seeking behavior; Geospatial distribution; Medical treatment preference
Predictors of acute intracranial hemorrhage and recurrence of chronic subdural hematoma following burr hole drainage
Authors: Chen FuMei , Wang Ke , Xu KangLi , Wang Li , Zhan TianXiang , Cheng Fei , Wang Hao , Chen Zuo-Bing , Gao Liang , Yang Xiaofeng
Published: February 2020

Objective To investigate predictors of postoperative acute intracranial hemorrhage (AIH) and recurrence of chronic subdural hematoma (CSDH) after burr hole drainage. Methods A multicenter retrospective study of patients who underwent burr hole drainage for CSDH between January 2013 and March 2019. Results A total of 448 CSDH patients were enrolled in the study. CSDH recurrence occurred in 60 patients, with a recurrence rate of 13.4%. The mean time interval between initial burr hole drainage and recurrence was 40.8±28.3 days. Postoperative AIH developed in 23 patients, with an incidence of 5.1%. The mean time interval between initial burr hole drainage and postoperative AIH was 4.7±2.9 days. Bilateral hematoma, hyperdense hematoma and anticoagulant drug use were independent predictors of recurrence in the multiple logistic regression analyses. Preoperative headache was an independent risk factor of postoperative AIH in the multiple logistic regression analyses, however, intraoperative irrigation reduced the incidence of postoperative AIH. Conclusions This study found that bilateral hematoma, hyperdense hematoma and anticoagulant drug use were independently associated with CSDH recurrence. Clinical presentation of headache was the strongest predictor of postoperative AIH, and intraoperative irrigation decreased the incidence of postoperative AIH.
Impact of Surgeon Handedness in Manual and Robot-assisted Total Hip Arthroplasty
Authors: Kong Xiangpeng , Yang Minzhi , Li Xiang , Ni Ming , Zhang Guoqiang , Chen Jiying , Wei Chai
Published: February 2020

Background: The purpose of this study was to examine whether surgeon handedness could affect cup positioning in manual total hip arthroplasty (THA), and whether robot could diminish or eliminate the impact of surgeon handedness on cup positioning in robot-assisted THA.Methods: Fifty-three patients who underwent bilateral robot-assisted THA and sixty-two patients who underwent bilateral manual THA between August 2018 and July 2019 in our institute were respectively analyzed in this study. When the difference between the bilateral anteversion or inclination was greater than 5°, the patient was regarded as having different cup positioning between bilateral THA. Their demographics, orientation of acetabular cup and postoperative 3 month Harris hip score (HHS) were recorded for analysis. Results: There were no significant difference in the gender, age, BMI, diagnosis’s composition, preoperative and postoperative HHS between the robotic and manual group. Two left hips dislocated in the manual group. The anteversion of left hip was significantly larger than that of right hip (24.77±10.44 vs 22.44±8.67, p=0.043) in the manual group. There were no significant difference of cup positioning between bilateral robot-assisted THA. The patients in manual group were significantly more likely to have different cup positioning between bilateral hips than those in robotic group (77% vs 45%, p=0.000). More manual THA were located out of the target zone than robot-assisted THA (70% vs 48%, p=0.001). Conclusions: Surgeon’s handedness showed a trend towards an impact on cup positioning in manual THA and robot might help surgeon eliminate the adverse impact. However, the impact of handedness on the clinical outcomes still needs further observation.
Collagen Coating for Tissue Culture
Authors: McCullough Shaun D.
Published: February 2020

This protocol is intended for collagen coating of tissue culture dishes and Transwell® multiple well plates. The attached methods document is a formal version of the information included here.Disclaimer: The information presented here has been reviewed and approved for publication by the US Environ do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
FITC-Dextran Trans-Epithelial Permeability Assay
Authors: Faber Samantha C. , McCullough Shaun D.
Published: February 2020

This protocol describes the use of the Fluorescein isothiocyanate (FITC)–dextran trans-epithelial permeability assay to quantify the effects of chemicals on epithelial cell monolayer permeability.The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
Increasing access to CBT for psychosis patients: a feasibility, randomized controlled trial evaluating brief, targeted CBT for distressing voices delivered by Assistant Psychologists (GiVE2)
Authors: Hayward Mark , Berry Clio , Cameron Ben , Arnold Kate , Berry Katherine , Bremner Stephen , Cavanagh Kate , Fowler David , Gage Heather , Greenwood Kathryn , Hazell Cassie , Jones Anna-Marie , Robertson Sam , Strauss Clara
Published: February 2020

Background

The National Institute for Health and Care Excellence (NICE) recommends that Cognitive Behaviour Therapy for psychosis (CBTp) is offered to all patients with a psychosis diagnosis. However, only a minority of psychosis patients in England and Wales are offered CBTp. This is attributable, in part, to the resource-intensive nature of CBTp. One response to this problem has been the development of CBTp in brief formats that are targeted at a single symptom and the mechanisms that maintain distress. We have developed a brief form of CBTp for distressing voices and reported preliminary evidence for its effectiveness when delivered by highly trained therapists (Clinical Psychologists). This study will investigate the delivery of this intervention by a cost-effective workforce of Assistant Psychologists following a brief training and evaluate the acceptability and feasibility of conducting a future, definitive, randomised control trial (RCT).

Methods

This is a feasibility study for a pragmatic three-arm parallel group superiority 1:1:1 RCT comparing a Guided self-help CBT intervention for voices and treatment as usual (GiVE) to Supportive Counselling and treatment as usual (SC) to Treatment as usual alone (TAU), recruiting across two sites, with blinded post-treatment and follow-up assessments. A process evaluation will quantitatively and qualitatively explore stakeholder experience.

Discussion

Expected outcomes will include an assessment of the feasibility of conducting a definitive RCT and data to inform the calculation of its sample size. If evidence from a subsequent fully powered RCT suggests that GiVE is clinically and cost effective when delivered by briefly trained Assistant Psychologists, CBTp offered in these less resource-intensive forms has the potential to generate benefits for individual patients (reduced distress, enhanced recovery and enhanced quality of life), service-level patient benefit (increased access to evidence-based psychological therapies) and economic benefits to the NHS (in terms of the reduced use of mental health inpatient services).
Disclosure of HIV seropositivity to sexual partner and psychosocial factors in Ethiopia: Systematic review and meta-analysis 
Authors: Endalamaw Aklilu , Geremew Demeke , Belete Habte , Dachew Berihun Assefa , Habtewold Tesfa Dejenie , Habtewold Tesfa Dejenie , Wilson Rhonda
Published: February 2020

Background: The burden of HIV/AIDS again becomes a public health problem after substantial control of its transmission in Ethiopia. For effective HIV transmission control measures, sero-disclosure to sexual partner is indispensable. Once the infection is established, psychosocial factors would have a great influence on HIV disclosure status to sexual partners which is very important to control viral transmission. This review aimed to estimate the national proportion of HIV disclosure practice to sexual partner and identify associated psychosocial factors.Methods: We searched PubMed, Scopus, African Journals Online, and Google Scholar databases. The Newcastle Ottawa quality assessment scale was used to assess the quality of studies. To ensure the absence or presence of publication bias, we used a funnel plot and performed Egger’s regression test for the subjective and objective assessment, respectively. Variation across studies was assessed using the I2 statistic. The pooled proportion was estimated by using weighted inverse variance random-effects model meta-analysis. We did subgroup and sensitivity analysis to explore the reason for heterogeneity and the impact of outlier finding on the overall estimation, respectively. Trend analysis was also performed to see the presence of time variation. Results: The proportion of HIV sero-disclosure practice to sexual partners was 76.03 % (95% Confidence Interval (CI): 68.78, 83.27). Being on ART (AOR=6.19; 95% CI: 2.92, 9.49), cohabiting with partner (AOR=4.48; 95% CI: 1.24, 7.72), getting counseling (AOR=3.94; 95% CI: 2.08, 5.80), had discussion prior to HIV testing (AOR= 4.40; 95% CI: 2.11, 6.69), awareness of partner’s HIV status (AOR= 6.08; 95%CI: 3.05, 9.10),smooth relationship with partner (AOR=4.44; 95% CI:1.28, 7.61), and being member of anti-HIV association (AOR=3.70; 95% CI: 2.20, 5.20) facilitates HIV status disclosure.Conclusions: In Ethiopia, still more than one-fourth of HIV-infected adults did not disclose their HIV positivity status to sexual partners. Psychosocial factors were the contributing factors of HIV-positive status disclosure. Further work is still needed to increase HIV status disclosure so as to decrease the transmission rate of HIV in Ethiopia.
Collaborative Model of Care between Orthopaedics and Allied Healthcare Professionals Trial (CONNACT) – A Feasibility Study in patients with knee osteoarthritis using a mixed method approach
Authors: Tan Bryan , DING Benjamin Tze Keong , PEREIRA Michelle Jessica , SKOU Soren Thorgaard , THUMBOO Julian , CAR Josip
Published: February 2020

Background Osteoarthritis is a leading cause of global disability resulting in significant morbidity and cost to the healthcare system. Current guidelines recommend lifestyle changes such exercises and weight loss as first line treatment prior to surgical consideration. Our current model of care is inefficient with suboptimal allied health intervention for effective behaviour changes. A 12-week community based, individualized, multidisciplinary new model of care for knee osteoarthritis was developed in light of current deficiencies. Methods The primary aim of this study was to determine the feasibility of a full randomized controlled trial evaluating this new model of care using pre-defined progression criteria. The secondary aim was to optimize the intervention and study design through a process evaluation. A pilot randomized trial design using a mixed method approach was utilized. Progression criteria for a full trial including key domains of patient recruitment and retention, outcome measure acceptability and improvement, adverse events were developed. The primary outcome measure was the Knee Injury and Osteoarthritis Outcome Score (KOOS) at baseline and 12-weeks. Secondary outcomes included quality of life, functional and psychological assessments. Semi-structured interviews were conducted with the patients at 12-weeks. Results 20 patients (3 males, 17 females) were randomized (10 intervention, 10 control). Intervention arm patients reported better improvements in their knee function, quality of life, psychological outcome, dietary improvement and weight loss compared to the control arm at 12-weeks. Semi-structured interviews revealed several themes pertaining to feasibility and intervention optimization. 5 out of the 6 progression criteria’s domains were met. Conclusion This pilot has demonstrated the feasibility of a full randomized control trial investigating the potential effectiveness of the new proposed model of care for knee osteoarthritis using pre-defined progression criteria and process evaluation. Results from the qualitative study were used to modify and improve the intervention content, delivery model and study design for a large effectiveness-implementation hybrid randomized control trial that is currently underway.
Down-regulation of Protein Tyrosine Phosphatase Receptor Gamma (PTPRG) in Chronic Myeloid Leukemia Patients in The State of Qatar is Due to Aberrant DNA Methylation Mechanism
Authors: Ismail Mohamed , Samara Muthanna , Sayab Ali Al , Alsharshani Mohamed , Yassin Mohamed , Vezzalini Marzia , Tomasello Luisa , Monne Maria , Morsi Hisham , Qoronfleh Walid , Zaid Hatem , Cook Richard , Sorio Claudio , Modjtahedi Helmout , Al-Dewik Nader
Published: February 2020

Background: Several studies showed that the aberrant DNA methylations are involved in leukemia and cancer pathogenesis. PTPRG is a natural inhibitory mechanism that is found to be down-regulated in Chronic Myeloid Leukemia (CML) disease. The mechanism behind its down-regulation has not been fully elucidated. Aim: The study investigates the role of methylation as a possible mechanism of PTPRG under-expression in CML patients. Method: Peripheral blood samples from CML patients treated with tyrosine kinase inhibitors (TKIs) and healthy controls were collected. DNA was extracted and treated with bisulfite treatment, followed by PCR and sequencing of 25 of CpG in Promoter and 26 of CpG in the Intronic regions of PTPRG. Bisulfite-sequencing technique as a high-resolution method was employed. Results : CML groups (New Diagnosed and Failed treatment) have significantly higher methylation levels in the Promoter and Intron regions compared to the healthy group. There were also significant differences in methylation levels of CpG sites in the Promoter and Intronic regions amongst the groups. Conclusion: Aberrant methylation of PTPRG is documented and potentially is one of the possible mechanisms of PTPRG down-regulation in CML.
Prevalence and associated factors of anemia among children aged 6-59 months in Ethiopia: Evidence from the Ethiopia Demographic and Health Survey
Authors: Woya Ashenafi Abate , Tekile Abay Kassa
Published: February 2020

Anemia is one of the most widely spread public health problems, especially in developing countries including Ethiopia. Thus, the aim of this study was to assess the prevalence and associated factors of anemia among children aged 6-59 months in Ethiopia. This study utilized secondary data from the 2016 Ethiopia Demographic and Health Survey. The data were analyzed using by STATA software version 14. Ordinal logistic regression statistical analysis was used to identify risk factors of anemia. Out of 8385 children considered in this study, more than half (57.3%) of them were anemic: 262(3.1%) were severely anemic, 2447(29.2%) were moderately anemic, and 2100(25.0%) were mildly anemic. Mothers’ anemic status and children anemic status were highly associated. Accordingly, among severely anemic mothers, 26.8% of children were severely anemic, among moderately anemic mothers, 39.7% of children were moderately anemic. Mother’s anemic status, place of residence, child age, smoking status, religion, and region of residence were factors associated with a prevalence of childhood anemia. A of anemia among children aged 6-59 months in Ethiopia was a severe public health problem. Thus, creating awareness among mothers how to control and manage the anemia status of their children and Routine iron supplementation are recommended to reduce childhood anemia.
Clinical Outcomes and Factors of Single Attempt of Stent Retrieval during Endovascular Thrombectomy for Acute Ischemic Stroke
Authors: Liu Mingli , Liu Yang , Lin Lin , Li Yongli , Yan Feng , Wang Rui , Cai Jinquan , Li Ruiyan
Published: February 2020

Backgrounds: Single attempt of stent retrieval during endovascular thrombectomy (EVT) seems to be associated with favorable clinical outcomes of patients with acute ischemic stroke (AIS), but the relationship between single attempt and clinical outcomes or complications have not been characterized. In addition, the factors related to single retrieval attempts during EVT are undefined. Here, we investigated the relationship between single attempt of stent retrieval and the clinical outcomes or complications after EVT, and further investigated the factors associated with single attempt of stent retrieval in AIS patients.
Methods: This study enrolled consecutive patients with AIS treated by EVT with second-generation stent retriever devices from April 2016 to April 2019. Attempts of EVT were classified as single or multiple attempts of stent retrieval after filtrating all enrolled patients based on inclusion and exclusion criteria. We analyzed the clinical outcomes and complications between single attempt of stent retrieval and multiple attempts of stent retrieval, and the factors independently associated with a single attempt were identified through logistical regression analysis.
Results: We enrolled 143 patients, including 69 patients with single attempt of stent retrieval and 74 patients with multiple attempts of stent retrieval. More patients with single attempt of stent retrieval reached favorable clinical outcomes ( p =0.016; adjusted OR [95%CI]: 2.652 [1.204-5.843] ), and patients with single attempts of stent retrieval showed as a lower incidence of symptomatic intracranial hemorrhage (sICH) ( p =0.046; adjusted OR [95%CI]: 0.192 [0.038-0.973] ). Diabetes mellitus ( p =0.026; adjusted OR [95%CI]: 2.871 [1.137-7.249] ) was independently associated with single attempt of EVT with stent retrieval.
Conclusions: Single attempt of stent retrieval during EVT can decrease incidence of sICH and improve favorable clinical outcomes of patients with AIS. Those with diabetes mellitus may be more prone to single attempt of stent retrieval during EVT .
Unravelling the role of Mesenchymal Stem/Stromal Cells Secretome in intervertebral disc degeneration in a pro-inflammatory/degenerative ex vivo model
Authors: Ferreira JR , Teixeira GQ , Neto E , Ribeiro-Machado C , Silva AM , Caldeira J , Pereira C Leite , Bidarra S , Maia AF , Lamghari M , Barbosa MA , Gonçalves RM
Published: February 2020

Background: Mesenchymal stem/stromal cells (MSCs) have been increasingly used in clinical trials for intervertebral disc (IVD) degeneration. Here, we aimed to evaluate the potential of a cell-free approach to degenerated IVD, testing if MSCs secretome can stimulate a regenerative response by modulating the IVD inflammatory cascade.
Methods: Human bone marrow-derived MSCs were pre-conditioned with IL-1β (10 ng/mL) and low oxygen (6% O2). The secretome of MSCs (MSCsec) was collected after 48h. Bovine IVD tissue explants cultured in pro-inflammatory/degenerative conditions (needle puncture + IL-1β) were treated with MSCsec or co-cultured with MSCs.
Results: MSCsec obtained upon IL-1β-pre-conditioning, as well as MSCs co-culture, down-regulated gene expression of pro-inflammatory cytokines, bIL-6 and bIL-8 after 48h, in IVD. IVD matrix degrading enzymes, bMMP1 and bMMP3, were downregulated and upregulated, respectively, in the presence of MSCsec, but not MSCs. After 14 days, MSCsec-treated IVDs revealed increased aggrecan content at the protein level, contrarily to MSCs/IVD co-cultures. Interestingly, IL-1β-preconditioning only, but not IL-1β-IVD, increased gene expression of hADAMTS5 and hTIMP-1in MSCs. Additionally, conditioned medium from MSCsec-treated IVDs did not promote angiogenesis or neurogenesis. In MSCsec-treated IVD, an increase in MCP-3 and GCP-2 was observed, while SDF-1α, TNF-α, IGF-1, Eotaxin 3, FGF-9, MIP-1δ, IFN-γ, IL-5, TNF-β, IL-4, TGF-β1, IL-16, IGFBP-3 and IGFBP-4 were decreased, compared with MSCs/IVD co-cultures.
Conclusions: MSCsec obtained upon IL1β-preconditioning, present an immunomodulatory role in degenerated IVD, as well as MSCs. Nevertheless, MSCsec but not MSCs, potentiate aggrecan deposition in IVD in pro-inflammatory/degenerative conditions. This finding can open new perspective on the use of MSCsec as a cell-based/cell-free approach to LBP.
A retrospective study of lumbar spine fusion surgery with the Wiltse approach and the traditional approach
Authors: Cheng Weiyi , Zeng Xixi , Gui Tong
Published: February 2020

Background： This study aimed to compare the clinical results between the Wiltse approach and traditional approach in lumbar spine fusion. Methods: In this retrospective controlled study, from May 2016 to May 2017, 70 cases with lumbar spine fusion surgery for lumbar disc herniation or lumbar spondylolisthesis within Ⅱdegree were assigned to Wiltse approach (35 cases,18 males and 17 females; mean age 52 years) and traditional approach groups (35cases, 19 males, 16 females; 51 years). There were totally 38 cases of lumbar disc herniation and 32 cases of II- degree spondylolisthesis in two groups. The operation time, the amount of blood loss and post-operation drainage, the level of creatine phosphokinase (CK) and the reduction of cross sectional area (CSA) in magnetic resonance imaging (MRI) were recorded. Results: After the operation for the Wiltse approach group, there was less operation time, blood loss and post-operative drainage (p＜0.05) ;the peripheral blood CK levels was changed a lot in 1 day and 3 days after the operation. In the Wiltse group, the reduction of CSA was significantly lower than traditional approach group in MRI. Conclusion: For lumbar spinal fusion surgery, the Wiltse approach do have shorter operation time and smaller vertebral side muscle injury compared with the traditional approach. However, in determining the operation program, the performer should fully recognize that the Wiltse clearance may have different influence on the operation at different anatomical level.