bluuebunny/crossref_metadata_2025 huggingface dataset:
Ultrasonographically derived caudal vena cava parameters acquired in a standing position and lateral recumbency in healthy, lightly sedated cats: a pilot study
Authors: Hultman Tove M , Boysen Søren R , Owen Rebecca , Yozova Ivayla D
Published: December 2021

Objectives

The aim of this study was to determine the feasibility of ultrasonographically measuring the caudal vena cava (CVC) at the subxiphoid view of healthy, lightly sedated cats in a standing position and lateral recumbency.

Methods

This was a prospective, observational, experimental single-centre study. Twenty healthy research-purposed cats were enrolled. Two trained operators scanned each cat in two positions – standing and lateral recumbency – in a randomised order. CVC diameter was measured at the narrowest diameter during inspiration and at the widest diameter during expiration, at two anatomical locations along the CVC – where the CVC crosses the diaphragm (base) and 2 mm caudal to the diaphragm. The CVC collapsibility index (CVC-CI) was calculated for each site. Normalcy was assessed with a Shapiro–Wilk test. A one-way ANOVA with post-hoc Tukey’s test was used to compare inspiratory with expiratory values within and between groups. A paired t-test compared the CVC-CI between groups ( P ⩽0.05 indicated statistical significance). Spearman’s correlation and Bland–Altman analysis assessed inter-operator variability.

Results

All ultrasonographic data passed normalcy and were reported as mean ± SD. When compared with each other, inspiratory and expiratory values were statistically different for position, location and operator (all P <0.0001). There was no statistically significant difference between lateral recumbency or standing position for inspiratory, expiratory and CVC-CI values. Inter-operator variability was substantial, with operator 2 consistently obtaining smaller measurements than operator 1. The mean CVC-CI in lateral recumbency at the base was 24% for operator 1 and 37% for operator 2. For the same site in standing position, CVC-CI was 27% and 41% for operators 1 and 2, respectively.

Conclusions and relevance

This pilot study demonstrates that it is possible to ultrasonographically measure the CVC diameter in both lateral recumbency and a standing position in healthy, lightly sedated cats. However, measurements obtained are operator dependent with variability between individuals. Further studies are needed to determine if ultrasonographic CVC assessment will prove helpful in estimating intravascular volume status in cats.
Inertial contributions to friction measurements in thrust bearings
Authors: Schuh Jonathon K.
Published: December 2021

Surface textures decrease friction in lubricated sliding contact. Traditionally, the friction reduction for a given textured surface is determined by using the Reynolds equation, which neglects fluid inertia. However, as the separation and relative motion between the surfaces increase, inertia can affect the measured tangential and normal forces for flow over a textured surface, and thus cause the coefficient of friction to differ from the purely viscous, Stokes flow prediction. Here, the increase in torque and normal force between a moving plate and stationary textured surface, which simulates a textured thrust bearing, are calculated as a function of the Reynolds number in the thin film limit. The predictions for a non-textured thrust bearing are compared to fully 3-D numerical simulations of the incompressible Navier-Stokes equation, and the predictions for textured thrust bearings are compared to experimental data given in the literature. Good agreement is seen between the predictions and the data, validating the predicted scaling laws. This work also suggests that inertia can be used as a secondary effect to reduce friction in lubricated sliding, and textures that take advantage of the inertial effects will have lower friction than textures that only use purely viscous effects.
Early childhood family background predicts meal frequency behaviour in children: Five-year follow-up study
Authors: Parikka Suvi , Martelin Tuija , Karvonen Sakari , Levälahti Esko , Kestilä Laura , Laatikainen Tiina
Published: December 2021

Aims:

Childhood nutrition patterns have an important role in later health. We studied the role of family type, other family background factors and their changes over a five-year follow-up with respect to meal frequency among children.

Methods:

Longitudinal data were collected in 2007–2009 and 2013–2014. A nationally representative sample of Finnish children ( n = 1822) aged 0.5–5 years at baseline and 5–10 years at follow-up and their families were used. The participation rate was 83% at baseline and 54% at follow-up. Meal frequency was defined as four to six meals per day. The associations of meal frequency with family background factors over a five-year follow-up period were examined by bivariate and multivariate regression analyses.

Results:

Eighty-nine per cent of the 5–10-year-old boys and girls had the recommended meal frequency at follow-up. Living in a single-parent family at baseline increased the risk of not eating the recommended number of meals compared with those living in intact families. After adjustments, a mother’s low level of education (OR 0.51, CI 0.29–0.93) and a decrease in income sufficiency (OR 0.54, CI 0.35–0.84) during the follow-up period were unfavourably associated with the recommended meal frequency. The difference between children in stable single-parent, reconstituted or joint physical custody families and those living in stable intact families remained significant when controlling for other variables.

Conclusions:

Single-parent families with a low socioeconomic position represent important target groups for interventions designed to promote regular meal frequency.
Socioeconomic status, lifestyle factors and asthma prevalence: results from a population-based study in Sweden
Authors: Molarius Anu , Hasselgren Mikael
Published: December 2021

Aim:

To examine differences in the prevalence of self-reported diagnosed asthma by socioeconomic status and the contribution of lifestyle factors to these differences.

Methods:

The study was based on 28,531 persons aged 18 years or older who answered a survey questionnaire sent to a random population sample in mid-Sweden in 2017. The overall response rate was 44%. Socioeconomic status was measured with educational level and economic difficulties, and lifestyle factors with physical activity, smoking, snuff use, risk-drinking of alcohol and obesity. The associations between socioeconomic status and asthma were analysed using multivariate logistic regression.

Results:

The overall asthma prevalence was 9% among women and 7% in men and decreased with increasing age. Educational level was not independently associated with asthma, but a statistically significant odds ratio (with 95% confidence intervals) for the prevalence of asthma was observed for economic difficulties 1.5 (1.3–1.7). Also, physical inactivity 1.2 (1.1–1.3) and obesity 1.6 (1.4–1.8) were associated with increased asthma prevalence. Smoking and risk-drinking were not statistically significantly associated with asthma whereas snuff users had a higher prevalence of asthma among women. Adjusting for lifestyle factors did not affect the association between socioeconomic status and asthma.

Conclusions:

In this population-based study, self-reported diagnosed asthma was independently associated with economic difficulties but not with educational level. Lifestyle factors did not explain the association between economic difficulties and asthma prevalence. This applies to both men and women as well as younger and older age groups.
Playing platformized language games: Social media logic and the mutation of participatory cultures in Chinese online fandom
Authors: Yin Yiyi , Xie Zhuoxiao
Published: December 2021

This study discusses the shifting dynamics of fan participatory cultures on social media platforms by introducing the concept of “platformized language games.” We conceive of a fan community as a “speech community” and propose that the language and discourses of fan participatory cultures are technological practices that only make sense in use and interactions as “games” on social media platform. Based on an ethnography of communication on fan communities on Weibo, we analyze the technological-communicative acts of fan speech communities, including the platformized setting, participants, topics, norms, and key purposes. We argue that the social media logic (programmability, connectivity, popularity, and datafication) articulates with fans’ language games, thus shifting the “form of life” of celebrity fans on social media. Empirically, fan participatory cultures continue to mutate in China, as fan communities create idiosyncratic platformized language games based on the selective appropriation of the social media logics of connectivity and data-driven metrics.
Dementia and sexuality in long-term care: Incompatible bedfellows?
Authors: Grigorovich Alisa , Kontos Pia , Heesters Ann , Martin Lori Schindel , Gray Julia , Tamblyn Watts Laura
Published: December 2021

Despite the recognized benefits of sexual expression and its importance in the lives of people living with dementia, research demonstrates that there are multiple barriers to its positive expression (e.g., expression that is pleasurable and free of coercion, discrimination, and violence) in RLTC homes. These barriers constitute a form of discrimination based on age and ability, and violate the rights of persons living with dementia to dignity, autonomy, and participation in everyday life and society. Drawing on a human rights approach to dementia and sexual expression, we explored the experiences of diverse professionals, family members, and persons living with dementia with explicit attention to the ways in which macro-level dynamics are influencing the support, or lack thereof, for sexual expression at the micro level. Focus groups and in-depth interviews were conducted with 27 participants, and the collected data were analyzed thematically. While all participants acknowledged that intimacy and sexual expression of persons living with dementia should be supported, rarely is such expression supported in practice. Micro-level factors included negative attitudes of professionals toward sexual expression by persons living with dementia, their discomfort with facilitating intimacy and sexual expression in the context of their professional roles, their anxieties regarding potential negative reactions from family members, and concerns about sanctions for failing to prevent abuse. In our analysis, we importantly trace these micro-level factors to macro-level factors. The latter include the cultural stigma associated with dementia, ageism, ableism, and erotophobia, all of which are reproduced in, and reinforced by, professionals’ education, as well as legal and professional standards that exclusively focus on managing and safeguarding residents from abuse. Our analysis demonstrates a complexity that has enormous potential to inform future research that is critically needed for the development of educational initiatives and to promote policy changes in this area.
High-quality RNA improves sensitivity of SARS-CoV-2 detection by colorimetric RT-LAMP
Authors: Puigmulé Marta , Coll Mònica , Pérez-Serra Alexandra , López Laura , Picó Ferran , Neto Nuria , Corona Mònica , Pinsach-Abuin Mel·lina , Ferrer-Costa Carles , Buxó Maria , Queralt Francesc-Xavier , Brugada Ramon
Published: December 2021

The global SARS-CoV-2 pandemic requires a rapid, reliable, and user-friendly diagnostic test to help control the spread of the virus. Reverse transcription and quantitative PCR (RT-qPCR) is currently the gold standard method for SARS-CoV-2 detection. Here, we develop a protocol based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and demonstrate increased sensitivity of this technique using fresh RNA extracts compared to RNA samples subjected to freezing/thawing cycles. We further compare RT-LAMP to RT-qPCR and demonstrate that the RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct values between 8 and 35.
Association between serum placental growth factor and vascular endothelial function in hyprtensive disorders complicating pregnancy
Authors: Li Weiling , Liu Shasha , Li Yuan , Wang Weijing , Dong Yiming , Qi Lin , Ren Jie , Zhang Jiefang
Published: December 2021

Hypertensive disorders complicating pregnancy (HDCP) is a systemic disease among pregnant women. Therefore, the prevention and prediction of hypertension during pregnancy are critical. This study aimed to clarify whether the vascular endothelial function of women with gestational hypertension was linked to placental growth factor. A total of 200 pregnant women were enrolled in our study and subsequently divided into two groups: the HDCP group and the normal pregnancy controls. The levels of serum placental growth factor, as well as plasma endothelin-1 and nitric oxide, between the two groups were measured. In addition, the endothelial function indexes, including pressure-strain elasticity coefficient (EP), the common carotid stiffness index (β), arterial compliance, single-point pulsed-wave velocity, and augment index (AI) of bilateral common carotid arteries, were compared between the HDCP and control groups using the echo tracking technique. In our study, the level of placental growth factor in the HDCP group was significantly lower than the control group. Furthermore, our results clarified that endothelin-1 increased while nitric oxide decreased in the HDCP group compared with the control group. On the other hand, we found that EP, β, pulsed-wave velocity and augment index values were significantly higher in the HDCP group than in the control group (P < 0.001). However, the value of arterial compliance was significantly decreased in patients of the HDCP group compared with the control group (P < 0.001). In conclusion, the association between serum placental growth factor and vascular endothelial function in HDCP could serve as a more accurate predictive factor of pregnant hypertension.
Survival stratification for colorectal cancer via multi-omics integration using an autoencoder-based model
Authors: Song Hu , Ruan Chengwei , Xu Yixin , Xu Teng , Fan Ruizhi , Jiang Tao , Cao Meng , Song Jun
Published: December 2021

Prognosis stratification in colorectal cancer helps to address cancer heterogeneity and contributes to the improvement of tailored treatments for colorectal cancer patients. In this study, an autoencoder-based model was implemented to predict the prognosis of colorectal cancer via the integration of multi-omics data. DNA methylation, RNA-seq, and miRNA-seq data from The Cancer Genome Atlas (TCGA) database were integrated as input for the autoencoder, and 175 transformed features were produced. The survival-related features were used to cluster the samples using k-means clustering. The autoencoder-based strategy was compared to the principal component analysis (PCA)-, t-distributed random neighbor embedded (t-SNE)-, non-negative matrix factorization (NMF)-, or individual Cox proportional hazards (Cox-PH)-based strategies. Using the 175 transformed features, tumor samples were clustered into two groups (G1 and G2) with significantly different survival rates. The autoencoder-based strategy performed better at identifying survival-related features than the other transformation strategies. Further, the two survival groups were robustly validated using “hold-out” validation and five validation cohorts. Gene expression profiles, miRNA profiles, DNA methylation, and signaling pathway profiles varied from the poor prognosis group (G2) to the good prognosis group (G1). miRNA–mRNA networks were constructed using six differentially expressed miRNAs (let-7c, mir-34c, mir-133b, let-7e, mir-144, and mir-106a) and 19 predicted target genes. The autoencoder-based computational framework could distinguish good prognosis samples from bad prognosis samples and facilitate a better understanding of the molecular biology of colorectal cancer.
Structure from motion with planar homography estimation: a real-time low-bandwidth, high-resolution variant for aerial reconnaissance
Authors: Arnold Christian , Nykl Scott , Graham Scott , Leishman Robert
Published: December 2021

We propose a new algorithm variant for Structure from Motion (SfM) to enable real-time image processing of scenes imaged by aerial drones. Our new SfM variant runs in real-time at 4 Hz equating to an 80× computation time speed-up compared to traditional SfM and is capable of a 90% size reduction of original video imagery, with an added benefit of presenting the original two-dimensional (2D) video data as a three-dimensional (3D) virtual model. This opens many potential applications for a real-time image processing that could make autonomous vision–based navigation possible by completely replacing the need for a traditional live video feed. The 3D reconstruction that is generated comes with the added benefit of being able to generate a spatially accurate representation of a live environment that is precise enough to generate global positioning system (GPS) coordinates from any given point on an imaged structure, even in a GPS-denied environment.
A randomized block policy gradient algorithm with differential privacy in Content Centric Networks
Authors: Wang Lin , Xu Xingang , Zhao Xuhui , Li Baozhu , Zheng Ruijuan , Wu Qingtao
Published: December 2021

Policy gradient methods are effective means to solve the problems of mobile multimedia data transmission in Content Centric Networks. Current policy gradient algorithms impose high computational cost in processing high-dimensional data. Meanwhile, the issue of privacy disclosure has not been taken into account. However, privacy protection is important in data training. Therefore, we propose a randomized block policy gradient algorithm with differential privacy. In order to reduce computational complexity when processing high-dimensional data, we randomly select a block coordinate to update the gradients at each round. To solve the privacy protection problem, we add a differential privacy protection mechanism to the algorithm, and we prove that it preserves the [Formula: see text]-privacy level. We conduct extensive simulations in four environments, which are CartPole, Walker, HalfCheetah, and Hopper. Compared with the methods such as important-sampling momentum-based policy gradient, Hessian-Aided momentum-based policy gradient, REINFORCE, the experimental results of our algorithm show a faster convergence rate than others in the same environment.
Turkish and Moroccan Dutch women’s views of using a self-sampling kit for human papillomavirus testing as a tool for cervical cancer screening: What are the barriers and the motivators?
Authors: Hilverda Femke , Fissers Katleen , van den Broek Thijs
Published: December 2021

Objective:

This study explores barriers and motivators to use self-sampling kits for human papillomavirus testing for cervical cancer screening as perceived by Dutch women of Turkish and Moroccan origin living in the Netherlands.

Methods:

A total of 11 in-depth semi-structured interviews were conducted and structured according to the theory of planned behavior.

Results:

Findings suggest that self-sampling may lift important barriers hampering traditional cervical cancer screening, such as those related to shame and chastity. However, self-sampling raises its own barriers too. Most importantly, some women fear that self-sampling may harm virginity. Some women also do not feel confident about their ability to properly use the self-sampling kit, but fears about the inability to properly use it often fade away upon having seen the self-sampling kit. Moreover, results show that knowledge about cervical cancer and its origin is limited, which may undermine women’s willingness to participate in a screening program.

Conclusions:

These results suggest that communication strategies to encourage using self-sampling kits among women of Turkish and Moroccan origin could benefit from culturally sensitive approaches, for example, by placing emphasis on issues such as virginity and chastity. Consistent with a recent advice of the Health Council of the Netherlands, the kit could furthermore be sent to eligible women as a standard procedure, rather than upon request. This could reduce hassle and doubts about women’s ability to use the self-sampling kit. Finally, educating women about the importance of screening to prevent cervical cancer is needed to foster informed decision-making.
Keeping it together: A phase-field version of path-connectedness and its implementation
Authors: Dondl Patrick , Wojtowytsch Stephan
Published: December 2021

We describe the implementation of a topological constraint in finite-element simulations of phase-field models, which ensures path-connectedness of preimages of intervals in the phase-field variable. The constraint takes the form of an energetic penalty for a suitable geodesic distance between all pairs of points in the domain. The main application of our method presented here is a discrete steepest descent of a phase-field version of a bending energy with spontaneous curvature and additional surface area penalty. This leads to disconnected surfaces without our topological constraint but connected surfaces with the constraint. Numerically, our constraint is treated by first transforming the double integral over all pairs of points in the domain to a weighted graph structure and then using Dijkstra’s algorithm to calculate the distance between discrete connected components.
Conspiracy Theories about Infectious Diseases: An Introduction
Authors: Hong Ying-yi , Chan Hoi-Wing , Douglas Karen M.
Published: December 2021

Understanding why people believe conspiracy theories related to disease outbreaks and the consequences of such beliefs is critical for combating both the COVID-19 pandemic and its corresponding “infodemic.” In the introduction to this special issue on conspiracy theories about infectious diseases, the authors first provide a brief overview of the narratives of conspiracy theories related to COVID-19, followed by a review of extant theoretical frameworks regarding the psychology of conspiracy beliefs. Specifically, they discuss how epistemic, existential, and social needs contribute to the holding of conspiracy beliefs. Then, the authors summarize the major findings from the nine empirical articles featured in this issue, particularly how they shed light on the antecedents and consequences of disease-related conspiracy beliefs. They conclude by discussing future directions for the study of disease-related conspiracy beliefs.
Characteristics and Counseling Needs of Prospective First-Generation College Students: Implications for School Counseling
Authors: Um Byeolbee
Published: December 2021

This study examined the personal characteristics and counseling needs of high school seniors who were strongly committed to attending a 4-year university. The results of this comparison study revealed that prospective first-generation college students reported lower perceived academic ability and a higher need for counseling in academic planning and career preparation than prospective continuing-generation college students. Subsequent analysis for the first-generation group by race revealed significant group differences in the perceptions and needs of school counseling.
Knowledge and Awareness on the Risks of Pesticide Use Among Farmers at Pulau Pinang, Malaysia
Authors: Sabran Siti Hajar , Abas Azlan
Published: December 2021

Obtaining information about the knowledge and awareness of the risk of pesticide use among farmers is essential to reduce the risk to the environment and health. This study aims to measure the level of knowledge of the risk of pesticide use to the environment and health, the level of awareness of pesticide use and to evaluate the relationship between both sociodemographic and knowledge factors toward awareness of pesticide use. The study was conducted among 360 farmers from Pulau Pinang. The results showed that the farmers’ level of awareness on pesticide use was moderate, although their level of knowledge on the risks of pesticide use to the environment and health was high. Farmers higher awareness of the prohibition of using harmful pesticides but the awareness level on how to dispose of used pesticide containers was low. This is driven by the factor of moderate knowledge of the existence of pesticide residue. There is a strong relationship of awareness with the educational factor, a moderate relationship for the area of paddy field factor, and also for the experience factor. There is also significant moderate relationship for knowledge on the risks of pesticide use to the environment and health with awareness. The government should emphasize furthering the educational program, the management and safe operating procedures for pesticides, and giving incentives to farmers who lack knowledge and experience, especially to younger farmers, as well as reducing environmental taxes and giving an incentive to farmers who cultivate paddy fields on a large scale.
Advances on biodegradable zinc-silver-based alloys for biomedical applications
Authors: Xiao Ximei , Liu Enyang , Shao Jinlong , Ge Shaohua
Published: December 2021

The biodegradable metals have great potential for the biomedical applications, which could be gradually degraded, absorbed, or excreted in the human body, avoiding the removal though secondary surgery. Zinc-based alloys are novel series of degradable metals for medical applications, and they are gaining lots of attention in the research field of absorbable metals. Zinc-silver (Zn-Ag) alloys show superior mechanical strength, good biodegradability, biocompatibility, and antibacterial properties, which render them to be potential candidates for biomedical applications. In this paper, we reviewed the development of Zn-Ag alloys in terms of mechanical properties, degradabilities, biocompatibilities, antibacterial properties, and potential applications in dentistry.
Decellularized tracheal scaffolds in tracheal reconstruction: An evaluation of different techniques
Authors: Lei Chenyang , Mei Sheng , Zhou Chun , Xia Chen
Published: December 2021

In humans, the trachea is a conduit for ventilation connecting the throat and lungs. However, certain congenital or acquired diseases may cause long-term tracheal defects that require replacement. Tissue engineering is considered a promising method to reconstruct long-segment tracheal lesions and restore the structure and function of the trachea. Decellularization technology retains the natural structure of the trachea, has good biocompatibility and mechanical properties, and is currently a hotspot in tissue engineering studies. This article lists various recent representative protocols for the generation of decellularized tracheal scaffolds (DTSs), as well as their validity and limitations. Based on the advancements in decellularization methods, we discussed the impact and importance of mechanical properties, revascularization, recellularization, and biocompatibility in the production and implantation of DTS. This review provides a basis for future research on DTS and its application in clinical therapy.
Double trouble: pelvic pain associated with a dual presentation of endometriosis and granulomatous peritonitis
Authors: Mandell Hanna , Stuparich Mallory , Nahas Samar , Veve Robert , Behbehani Sadikah
Published: December 2021

A 34-year-old patient presented to our office with chronic pelvic pain. Prior laparoscopic surgery revealed endometriotic appearing lesions which were ablated. The patient opted for another surgery to diagnose and treat potential leftover endometriosis. Laparoscopy revealed the presence of endometriotic appearing lesions, but pathology revealed two different histological diagnosis: endometriosis and granulomas, even in areas where no endometriosis was encountered. In any granulomatous presentation, exposure to mycobacteria must be ruled out. Without excision of abnormal appearing lesions seen on laparoscopy, endometriosis can not be confirmed and other potential causes of pain cannot be excluded.
Evaluation of Shared Experiences Among Patients and Providers Following Behavioral Health Integration in Primary Care
Authors: Kenton Natalie , Bouranis Nicole , Cox Emily J. , Jacobson Laura , Wright Bill J
Published: December 2021

Behavioral health integration (BHI) changes the paradigm of primary care delivery by integrating behavioral healthcare into primary care. Thus, BHI likely alters the shared experiences of both patients and providers in an interrelated manner; however, their experiences are usually evaluated separately. The purpose of this study was to analyze these shared experiences together within patient–provider pairs in integrated clinics. First, patient interviews were conducted using semi-structured interview guides and transcripts were analyzed for major themes of patient experience. Next, providers named in patient interviews were interviewed around these same themes. Thematic analysis was performed on 18 transcripts (11 patients, 7 providers). Common themes included BHI experience, pain management, feeling heard by providers, and health care experiences. Areas of alignment included positive perception of BHI, an absence of long-term care, and a desire to share decision-making. Pain management was a persistent area of conflict, and the differing experiences were consistent with a change in the psychodynamic patient–provider model. This conflict highlights a gap in BHI and a need for provider education about psychodynamic relationship models.
The public and its assets: Performing appraisal and advocacy for blue and green infrastructure in London, England
Authors: Peters Helge , Landström Catharina
Published: December 2021

London's water sector professionals seek to secure public funds for blue and green infrastructure projects while also engaging with local advocates for river improvement. This paper argues that current project appraisal practice forms a barrier to aligning public investment with local demand because justifying investment requires the enactment of a utilitarian public good that is at odds with the non-instrumental values motivating local advocacy. Drawing on qualitative evidence and performativity theory, we show how the appraisal practices of water sector professionals and the environmental advocacy of London residents both enact publics in different arenas of water management: appraisal enacts a general public to secure funding, while advocacy enacts a particular public that serves to articulate local demand for environmental improvement. Whereas the performativity of a general public works through demonstrating nature's economic value to people, the performativity of local publics is animated by people's responsibility towards nature. We find that the general public that is enacted through cost-benefit analysis legitimizes public expenditure on readily demonstrable economic benefits, such as flood risk mitigation, while withholding funds from the water quality improvement valued by local publics. Comparing the performativities of general and local publics, we discuss the conditions under which appraisal and advocacy practices enact their respective underlying values. We conclude that the current appraisal practice frustrates the delivery of blue and green infrastructure projects that would respond to local demand for improving rivers.
Landscape plasticity and its erasure
Authors: Sturgeon Janet C.
Published: December 2021

For centuries, people who call themselves Akha had formed village landscapes of rotating shifting cultivation fields amid regenerating trees together with enduring wooded sites, all under the purview of their ancestors. In Mengsong, an Akha settlement on the ridge separating China and Burma, farmers had managed complex, biodiverse and flexible landscapes for 250 years. In 1996–1997, my extended research there identified cultivation patterns that I called landscape plasticity, referring to farming practices that were highly mutable over space and time, often transgressing state-allocated property lines and the international border with Burma. From 1997 to 2011, a combination of exclusionary state forest policies, the racialization of upland minorities, and a state poverty alleviation project brought landscape plasticity and the ancestors to an end. Using concepts from sentient landscapes, resource access, environmentalism, racialization, and capitalist markets, this paper seeks to explain how landscape plasticity and the ancestors were erased. At the same time, I explore the puzzle of why Akha farmers saw these contingent outcomes as positive
Platelet ACKR3/CXCR7 favors antiplatelet lipids over an atherothrombotic lipidome and regulates thromboinflammation
Authors: Cebo Malgorzata , Dittrich Kristina , Fu Xiaoqing , Manke Mailin C. , Emschermann Frederic , Rheinlaender Johannes , von Eysmondt Hendrik , Ferreirós Nerea , Sudman Jessica , Witte Alexander , Pelzl Lisann , Borst Oliver , Geisler Tobias , Rath Dominik , Bakchoul Tamam , Gawaz Meinrad , Schäffer Tilman E. , Lämmerhofer Michael , Chatterjee Madhumita
Published: December 2021

Platelet ACKR3/CXCR7 surface expression is enhanced and influences prognosis in coronary artery disease (CAD) patients, who exhibit a distinct atherothrombotic platelet lipidome. Current investigation validates the potential of ACKR3/CXCR7 in regulating thromboinflammatory response through its impact on the platelet lipidome. CAD patients with enhanced platelet ACKR3/CXCR7 expression exhibited reduced aggregation. Pharmacological CXCR7 agonist (VUF11207) significantly reduced prothrombotic platelet response in blood from acute coronary syndrome patients ex vivo. CXCR7 agonist administration reduced thrombotic functions and thromboinflammatory plateletleukocyte interactions post–myocardial infarction and arterial injury in vivo. ACKR3/CXCR7 ligation did not affect surface availability of surface receptors, coagulation profile, bleeding time, plasma-dependent thrombin generation (thrombinoscopy), or clot formation (thromboelastography) but counteracted activation-induced phosphatidylserine exposure and procoagulant platelet-assisted thrombin generation. Targeted (micro-UHPLC-ESI-QTrap-MS/MS) and untargeted (UHPLCESI-QTOF-MS/MS) lipidomics analysis revealed that ACKR3/CXCR7 ligation favored generation of antithrombotic lipids (dihomo-γ-linolenic acid [DGLA], 12-hydroxyeicosatrienoic acid [12-HETrE]) over cyclooxygenase-1 (COX-1) or 12-lipoxygenase (12-LOX) metabolized prothrombotic and phospholipase-derived atherogenic lipids in healthy subjects and CAD patients, contrary to antiplatelet therapy. Through 12-HETrE, ACKR3/CXCR7 ligation coordinated with Gαs-coupled prostacyclin receptor to trigger cyclic adenosine monophosphate/protein kinase A–mediated platelet inhibition. ACKR3/CXCR7 ligation reduced generation of lipid agonists and lipid signaling intermediates, which affected calcium mobilization, intracellular signaling, and consequently platelet interaction with physiological matrices and thromboinflammatory secretome. This emphasized its functional dichotomy from prothrombotic CXCR4. Moreover, CXCR7 agonist regulated heparin-induced thrombocytopenia–sera/immunoglobulin G–triggered platelet and neutrophil activation, heparin-induced platelet aggregation, generation of thromboinflammatory lipids, platelet-neutrophil aggregate formation, and thromboinflammatory secretion ex vivo. Therefore, ACKR3/CXCR7 may offer a novel therapeutic strategy in acute/chronic thromboinflammation exaggerated cardiovascular pathologies and CAD.
A Review on Friction Stir Welding of Steels
Authors: Mohan Dhanesh G , Wu ChuanSong
Published: December 2021

Friction Stir Welding (FSW) is the most promising solid-state metals joining method introduced in this era. Compared to the conventional fusion welding methods, this FSW can produce joints with higher mechanical and metallurgical properties. Formerly, FSW was adopted for low melting metals like aluminum alloys. In recent years it has made significant progress in friction stir welding of steels since unfavourable phase transformations occurred in welds due to the melting of the parent and filler metals in fusion welding can be eliminated. The main advantage of FSW over traditional fusion welding is the reduction in the heat-affected zone (HAZ), and the joints exhibit excellent mechanical and corrosion resistance properties. This article reviews the progress in the relevant issues such as the FSW tool materials and tool profiles for joining steels, microstructure and mechanical properties of steels joints, special problems in joining dissimilar steels. Moreover, in-situ heating sources was used to overcome the main limitations in FSW of hard metals and their alloys, i.e., tool damages and insufficient heat generation. Different in-situ heating sources like laser, induction heat, gas tungsten arc welding assisted FSW for various types of steels are introduced in this review. On the basis of the up-to-date status, some problems that need further investigation are put forward.
Genetic stability of Aedes aegypti populations following invasion by wMel Wolbachia
Authors: Lau Meng-Jia , Schmidt Thomas L. , Yang Qiong , Chung Jessica , Sankey Lucien , Ross Perran A. , Hoffmann Ary A.
Published: December 2021

Background

Wolbachia wMel is the most commonly used strain in rear and release strategies for Aedes aegypti mosquitoes that aim to inhibit the transmission of arboviruses such as dengue, Zika, Chikungunya and yellow fever. However, the long-term establishment of wMel in natural Ae. aegypti populations raises concerns that interactions between Wolbachia wMel and Ae. aegypti may lead to changes in the host genome, which could affect useful attributes of Wolbachia that allow it to invade and suppress disease transmission.

Results

We applied an evolve-and-resequence approach to study genome-wide genetic changes in Ae. aegypti from the Cairns region, Australia, where Wolbachia wMel was first introduced more than 10 years ago. Mosquito samples were collected at three different time points in Gordonvale, Australia, covering the phase before (2010) and after (2013 and 2018) Wolbachia releases. An additional three locations where Wolbachia replacement happened at different times across the last decade were also sampled in 2018. We found that the genomes of mosquito populations mostly remained stable after Wolbachia release, with population differences tending to reflect the geographic location of the populations rather than Wolbachia infection status. However, outlier analysis suggests that Wolbachia may have had an influence on some genes related to immune response, development, recognition and behavior.

Conclusions

Ae. aegypti populations remained geographically distinct after Wolbachia wMel releases in North Australia despite their Wolbachia infection status. At some specific genomic loci, we found signs of selection associated with Wolbachia, suggesting potential evolutionary impacts can happen in the future and further monitoring is warranted.
Multiplex polymerase chain reaction detection of Streptococcus pneumoniae and Haemophilus influenzae and their antibiotic resistance in patients with community-acquired pneumonia from southwest Iran
Authors: Farajzadeh Sheikh Ahmad , Rahimi Robab , Meghdadi Hossein , Alami Ameneh , Saki Morteza
Published: December 2021

Background

This study aimed to evaluate the occurrence ofStreptococcus pneumoniaeandHaemophilus influenzaein sputum of patients with community-acquired pneumonia (CAP) using culture and multiplex polymerase chain reaction (M-PCR) methods and to survey the antibiotic resistance patterns of aforesaid isolates.

Result

In total, 23.9 % (n= 22/92) of sputum samples showed positive results in the culture method.S. pneumoniaeandH. influenzaewere isolated from 15 (16.3 %) and 7 (7.6%) samples, respectively. Using M-PCR, 44 (47.8 %) samples were positive forS. pneumoniaeandH. influenzae. Of these,S. pneumoniaeandH. influenzaewere detected in 33 (35.8%) and 11 (11.9%) of the sputum samples, respectively.The sensitivity, specificity, and accuracy rates of PCR in detection ofS. pneumoniaein comparison with culture method were 100, 76.6, and 83.6%, respectively. While, the sensitivity, specificity, and accuracy rates of PCR in detection ofH. influenzaein comparison with culture method were 100, 95.3, and 95.8%, respectively. Out of 11 isolates ofH. influenzae, two strains confirmed asH. influenzaetype b (Hib) and 3 isolates were type f. However, 6 isolates were non-typable. The co-trimoxazole and amoxicillin/clavulanate were the less effective antibiotics againstS. pneumoniaandH. influenzae, respectively. Ceftriaxone with 13.3% resistance rates was the most effective antibiotic againstS. pneumoniae, while, clarithromycin, ceftriaxone, and gentamicin with resistance rates of 28.6% for each one were the most effective chemicals againstH. influenzaeisolates.

Conclusion

In this study, the prevalence ofS. pneumoniaewas more thanH. influenzaeusing culture and M-PCR methods. The M-PCR provided better efficiency in detecting the bacterial agents in CAP patients compared to culture method. This method can improve the early detection of pathogens contributed to CAP. The drug resistantS. pneumoniaeandH. influenzaeindicated the need to develop a codified monitoring program to prevent further spread of these strains.
Risk of gallstones based on ABCG8 rs11887534 single nucleotide polymorphism among Taiwanese men and women
Authors: Liang Keng-Wei , Huang Hsin-Hui , Wang Lee , Lu Wen-Yu , Chou Ying-Hsiang , Tantoh Disline Manli , Nfor Oswald Ndi , Chiu Neng-Yu , Tyan Yeu-Sheng , Liaw Yung-Po
Published: December 2021

Background

Gallstones are abnormal masses caused by impaired metabolism of cholesterol, bilirubin, or bile salts in the gallbladder or biliary tract. ATP-binding cassette subfamily G member 8 (ABCG8) is a protein that regulates cholesterol efflux from the liver. Genome-wide association studies (GWAS) and meta-analyses of GWAS revealed the ABCG8 rs11887534 variant as the most common genetic determinant of gallstones in humans. These findings have not been extensively replicated in Taiwanese. Therefore, we appraised the relationship between gallstones and rs11887534 in a relatively large Taiwanese sample.

Methods

We retrieved data collected through questionnaires, physical and biochemical tests from the Taiwan Biobank Bank (TWB). The study participants comprised 7388 men and 13,880 women who voluntarily enrolled in the Taiwan Biobank project between 2008 and 2019. Gallstones were self-reported.

Results

The overall sample size was 21,268 comprising 938 gallstone patients and 20,330 non-gallstone individuals. Among the participants, 20,640 had the GG and 628 had the GC + CC genotype. Atp-value < 0.05, the baseline genotypes and gallstone status between men and women were not significantly different. The risk of gallstones was higher in participants having the GC + CC compared to the GG genotype: odds ratio (OR); 95% confidence interval (CI) = 1.698; 1.240–2.325), but was lower in men compared to women (OR = 0.763; 95% CI = 0.638–0.913). Compared to men with the rs11887534 GG genotype, women with the GG and GC + CC genotypes had a higher risk of gallstone (OR; 95% CI = 1.304; 1.087–1.565 for GG and 2.291; 1.514–3.467 for GC + CC). The positive association between GC + CC and gallstones was retained after we restricted the analysis to the female participants (OR; 95% CI = 1.789 = 1.208–2.648). Hormone use was associated with an elevated risk of gallstones (OR; 95% CI = 1.359; 1.107–1.668). Relative to GG and no hormone use, we found a significantly high risk among hormone users with the GC + CC genotype (OR; 95% CI = 3.596; 1.495–8.650).

Conclusions

The rs11887534 GC + CC genotype was independently associated with a higher risk of gallstones. This risk was much higher among women, especially those who used hormones for various gynecological purposes.
Embedding audiological screening within memory clinic care pathway for individuals at risk of cognitive decline—patient perspectives
Authors: McDonough Anna , Dookhy Joshi , McHale Cathy , Sharkey Jennifer , Fox Siobhan , Kennelly Sean P.
Published: December 2021

Background

With the evolving knowledge on hearing as a potentially modifiable mid-life risk factor for dementia, identification of people at risk becomes increasingly important. People with mild cognitive impairment (MCI) presenting to specialist memory services represent a key “at-risk” target population for audiological evaluation, but few services have established this pathway. This study sought to examine the patient experience and understanding of this process.

Methods

All patients with MCI attending a tertiary referral memory service referred for audiology review were contacted. A patient survey was delivered over the phone. Outpatient letters and the memory clinic database were reviewed.

Results

Twenty patients with MCI were included in the survey. Eight (8/20, 40%) had self-reported hearing loss. Upon formal audiological assessment seventeen (17/20, 85%) had objective evidence of hearing loss; nine (9/17, 52.9%) with mild-moderate and eight (8/17, 47%) with moderate-severe hearing loss. Only six patients (6/20, 30%) recalled having the rationale behind having a hearing test as part of their memory work-up explained to them. However, the majority (15/20, 75%) felt a hearing test was an important part of their memory assessment. Just seven patients overall (7/20, 35%) identified a link between hearing-loss and cognition. All patients who provided feedback on the service itself made positive comments, although (4/20, 20%) felt they did not get adequate information about the results.

Conclusions

A significant proportion of people with MCI had de-novo evidence of hearing impairment upon assessment. Patients are satisfied with incorporating audiological evaluation into a memory clinic assessment, however clear communication around indication, recommendations, and follow-up ensuring compliance is required.
The value of multimodal ultrasonography in differential diagnosis of tuberculous and non-tuberculous superficial lymphadenitis
Authors: Chu Jie , Zhang Ying , Zhang Wenzhi , Zhao Dan , Xu Jianping , Yu Tianzhuo , Yang Gaoyi
Published: December 2021

Background

To investigate the value of multimodal ultrasonography in differentiating tuberculosis from other lymphadenopathy.

Methods

Sixty consecutive patients with superficial lymphadenopathy treated at our hospital from January 2017 to December 2018 were categorized into four types based on the color Doppler ultrasound, five types based on contrast-enhanced ultrasound, and five types based on elastography. Sensitivity and specificity were calculated of all the three imaging, including color Doppler examination, contrast-enhanced ultrasound and one individual multimodal method, for detecting lymph nodes.

Results

A total of 60 patients were included in the final analysis. Of those, Mycobacterium tuberculosis was positive in 38 patients and negative in 22 patients. Among the 38 patients who were positive for Mycobacterium tuberculosis, of which 23 had a history of pulmonary tuberculosis, accounting for 60.53% of the positive cases, and the remaining patients did not combine lesions of other organs. Among the 60 superficial lymph nodes, 63.3% presented with tuberculous lymphadenitis. The sensitivity, specificity, and accuracy of the color Doppler examination were 73.68%, 68.18%, and 71.67%, respectively. The sensitivity, specificity and accuracy of contrast-enhanced ultrasound were 89.47%, 63.64% and 80.00%, respectively. The sensitivity, specificity and accuracy of the elastography were 63.16%, 63.64% and 63.33%, respectively. The sensitivity, specificity and accuracy of one individual multimodal method were 42.11%, 95.45% and 61.67%, respectively. The sensitivity, specificity and accuracy of all modes combined were 100.00%, 27.27% and 73.33%, respectively.

Conclusion

Multimodal ultrasonography has high predictive value for the differential diagnosis of superficial tuberculous lymphadenitis.