bluuebunny/crossref_metadata_2025 huggingface dataset:
Monitoring the micellar packing of photo-crosslinkable Pluronic F127 dimethacrylate during 3D printing
Authors: Bomediano Mateus P. , da Silva Laura C. E. , Mota-Santiago Pablo , de Oliveira Marcelo G. , Plivelic Tomás S.
Published: March 2024

Pluronic F127 dimethacrylate (F127-DM) inks exhibit potential for 3D printing biomaterials due to their combined gelation and photo-crosslinking capabilities. Previous studies have explored their application in extrusion 3D printing of medical devices, relying on their long-range micellar ordering to impart mechanical stability, stretchability, and control over drug delivery. However, there is a lack of information regarding the impact of the extrusion and photo-crosslinking processes on the micellar ordering of F127-DM. Herein, we employed in operando 3D printing synchrotron small-angle X-ray scattering (SAXS) to analyze the influence of various printing parameters, including nozzle shape, nozzle size, extrusion rate, ink composition, and photo-crosslinking, on the micellar ordering of F127-DM. Our findings from 2D SAXS patterns indicate orientation of the micellar packing clusters along the flow direction. The nozzle diameter and shape emerged as crucial parameters, leading to increased disruption of long-range ordering. Furthermore, 1D SAXS curves during UV-Vis light exposure and photo-crosslinking revealed a partial preservation of the micellar packing structure, with cluster size reduction, and coexisting with randomly distributed micelles and unimers. These results underscore the importance of in operando synchrotron experiments for the systematic study of 3D printing parameters from a nanostructure perspective.
Yaw Stability Control of Unmanned Emergency Supplies Transportation Vehicle Considering Two-Layer Model Predictive Control
Authors: Tang Minan , Zhang Yaqi , Wang Wenjuan , An Bo , Yan Yaguang
Published: March 2024

The transportation of emergency supplies is characterized by real-time, urgent, and non-contact, which constitute the basic guarantee for emergency rescue and disposal. To improve the yaw stability of the four-wheel-drive unmanned emergency supplies transportation vehicle (ESTV) during operation, a two-layer model predictive controller (MPC) method based on a Kalman filter is proposed in this paper. Firstly, the dynamics model of the ESTV is established. Secondly, the improved Sage–Husa adaptive extended Kalman filter (SHAEKF) is used to decrease the impact of noise on the ESTV system. Thirdly, a two-layer MPC is designed for the yaw stability control of the ESTV. The upper-layer controller solves the yaw moment and the front wheel steering angle of the ESTV. The lower-layer controller optimizes the torque distribution of the four tires of the ESTV to ensure the self-stabilization of the ESTV operation. Finally, analysis and verification are carried out. The simulation results have verified that the improved SHAEKF can decrease the state estimation error by more than 78% and achieve the noise reduction of the ESTV state. Under extreme conditions of high velocity and low adhesion, the average relative error is within 6.77%. The proposed control method can effectively prevent the instability of the ESTV and maintain good yaw stability.
Correction: Fu, J.; Tong, G. The State of Grain Trade between China and Russia: Analysis of Growth Effect and Its Influencing Factors. Agriculture 2023, 13, 1407
Authors: Fu Jing , Tong Guangji
Published: March 2024

In the published publication [...]
Correction: Gonçalves Vero et al. Transport of Pigs of Two Market Weights at Two Space Allowances: Effects on Behaviour, Blood Parameters, and Meat Quality under Summer and Winter Conditions. Animals 2023, 13, 2767
Authors: Gonçalves Vero Jessica , Devillers Nicolas , Bridi Ana Maria , Moak Kyle A. T. , Aboagye Gizella , Agostinis Ferreira Guilherme , Genova Jansller Luiz , Conte Sabine , Faucitano Luigi
Published: March 2024

In the original publication [...]
Comparative Analysis of the Systematics and Evolution of the Pampus Genus of Fish (Perciformes: Stromateidae) Based on Osteology, Population Genetics and Complete Mitogenomes
Authors: Zhang Cheng , Liu Hanjing , Huang Xiang , Yuan Zi , Zhang Shun , Xu Shanliang , Liu Jing , Wang Yajun , Wang Danli , Hu Jiabao
Published: March 2024

Pampus is a widespread species of fish in the western Pacific and Indian Oceans that has significant commercial worth. Its evolutionary history and phylogenetics are still poorly understood, and details on its intraspecific taxonomy are debatable, despite some morphological and molecular research. Here, we analyzed this species using skeletal structure data as well as nuclear (S7 gene) and mitochondrial genetic information (COI, D-loop and mitogenomes). We found that the genetic distance between P. argenteus and P. echinogaster was much smaller than that between other Pampus species, and both maximum likelihood and Bayesian phylogenetic trees yielded almost identical tree topologies. An additional and adjacent M repeat was found in the downstream region of the IQM gene cluster of P. argenteus and P. echinogaster, and the trnL2 gene of P. minor was translocated. The genus Pampus experienced early rapid radiation during the Palaeocene with major lineages diversifying within a relatively narrow timescale. Additionally, three different methods were conducted to distinguish the genus Pampus species, proving that P. argenteus and P. echinogaster are the same species, and P. liuorum is speculated to be a valid species. Overall, our study provides new insights not only into the evolutionary history of Pampus but its intraspecific taxonomy as well.
Platform-Specific Fc N-Glycan Profiles of an Antisperm Antibody
Authors: Nador Ellena , Xia Chaoshuang , Santangelo Philip J. , Whaley Kevin J. , Costello Catherine E. , Anderson Deborah J.
Published: March 2024

IgG Fc N-glycosylation is necessary for effector functions and is an important component of quality control. The choice of antibody manufacturing platform has the potential to significantly influence the Fc glycans of an antibody and consequently alter their activity and clinical profile. The Human Contraception Antibody (HCA) is an IgG1 antisperm monoclonal antibody (mAb) currently in clinical development as a novel, non-hormonal contraceptive. Part of its development is selecting a suitable expression platform to manufacture HCA for use in the female reproductive tract. Here, we compared the Fc glycosylation of HCA produced in two novel mAb manufacturing platforms, namely transgenic tobacco plants (Nicotiana benthamiana; HCA-N) and mRNA-mediated expression in human vaginal cells (HCAmRNA). The Fc N-glycan profiles of the two HCA products were determined using mass spectrometry. Major differences in site occupancy, glycan types, and glycoform distributions were revealed. To address how these differences affect Fc function, antibody-dependent cellular phagocytosis (ADCP) assays were performed. The level of sperm phagocytosis was significantly lower in the presence of HCA-N than HCAmRNA. This study provides evidence that the two HCA manufacturing platforms produce functionally distinct HCAs; this information could be useful for the selection of an optimal platform for HCA clinical development and for mAbs in general.
Effects of Dietary Eucommia ulmoides Leaf Extract Supplementation on Growth Performance, Meat Quality, Antioxidant Capacity, and Lipid Metabolism of Finishing Pigs
Authors: Han Mengmeng , Yin Yunju , Gong Saiming , Shi Hanjing , Li Qilong , Lian Xiao , Duan Yehui , Li Fengna , Guo Qiuping
Published: March 2024

This study aimed to investigate the effects of dietary Eucommia ulmoides leaf extract (ELE) on meat quality, antioxidant capacity, and lipid metabolism in finishing pigs. A total of 240 “Duroc × Landrace × Yorkshire” crossbred pigs with an initial weight of 74.70 ± 0.77 kg were randomly assigned to two groups: control group and 0.2% ELE group, with each group containing 10 replicates of 12 pigs per pen (half barrows and half gilts). The data showed dietary 0.2% ELE supplementation did not affect growth performance but tended to reduce the backfat thickness of the finishing pigs (p = 0.07). ELE diets increased pH value (p < 0.05) and meat color score (p = 0.01) and decreased 45 min L* value (p < 0.05), 24 h L* value (p = 0.01), pressurization loss (p = 0.01), and 24 h drip loss (p < 0.05) in longissimus dorsi (LD) muscle, accompanied by an increased (p < 0.05) proportion of monounsaturated fatty acids (MUFA) and decreased polyunsaturated fatty acids (PUFA) (p = 0.06) and n-6/n-3 PUFA ratio (p = 0.05) compared to controls. In addition, ELE supplementation increased inosine monophosphate (IMP) (p = 0.01), sweet amino acids (AAs) (p < 0.05), and total free AA content (p = 0.05) in LD. Meanwhile, increased activity of glutathione peroxidase (p < 0.05) and superoxide dismutase (p < 0.01) in both serum and LD muscle and decreased malondialdehyde content (p < 0.01) in LD muscle were detected with ELE treatment. Moreover, pigs fed ELE had a higher total protein (p < 0.01), albumin (p < 0.05), and high-density lipoprotein cholesterol (p < 0.05) and a lower total cholesterol (p < 0.01) and triacylglycerols (p = 0.06) in serum. Consistently, significant effects of dietary ELE were observed on the relative mRNA expression of lipid metabolism in the backfat and the LD muscle, respectively. ELE attenuated lipogenic processes in backfat, decreasing the relative expression of acetyl-CoA carboxylase and upregulating the relative expression of adipose triacyl glyceride lipase, carnitine palmitoyl transferase 1B, and fatty acid-binding protein 4 (p < 0.05). ELE also decreased the relative expression of CCAAT/enhancer-binding protein α (p < 0.05), fatty acid translocase (p < 0.05), carnitine palmitoyl transferase 1B (p < 0.01), and adipose triacyl glyceride lipase (p < 0.05) in LD muscle (p < 0.05). More specifically, lipogenesis appeared to be inhibited in both LD muscle and backfat, with the difference being that lipolysis was enhanced in backfat and inhibited in LD muscle. In conclusion, dietary ELE supplementation can potentially enhance carcass traits, sensory quality, and nutritional value of pork without negatively affecting intramuscular fat content. The underlying mechanism for these positive effects may be linked to the alterations in lipid metabolism and increased antioxidant capacity induced by ELE.
The Efficacy and Safety of GF101 and Its Antioxidant Effect on In Vitro Fertilization Outcomes: A Double-Blind, Non-Inferiority, Randomized, Controlled Trial with Coenzyme Q10
Authors: Shin So Yeon , Yoon Hye Kyung , Kim Jee Hyun , Kim Ji Hyang , Park Chan , Choi Dong Hee , Yu Young Dong , Shin Ji Eun , Kwon Hwang
Published: March 2024

(1) Background: Oxidative stress adversely affects fertility by impairing oocyte fertilization potential, primarily due to meiotic segregation errors and cohesion loss. Superoxide dismutase (SOD) and Coenzyme Q10 (CoQ10) are prominent antioxidants known to mitigate oxidative damage. (2) Methods: This study recruited 86 patients undergoing in vitro fertilization (IVF) at a single center for a 12-week, randomized, double-blind, active-comparator-controlled trial. Participants were allocated into two groups: one receiving CoQ10 as an antioxidant (the CoQ10 group) and the other receiving GF Bacillus antioxidative enzyme SOD (the GF101 group). The primary endpoints were changes in serum oxidative markers (SOD and catalase) and IVF outcomes, including clinical pregnancy, miscarriage, and live birth rates. Follicular fluid (FF) SOD and catalase concentrations on the day of retrieval, the metaphase II (MII) oocyte rate, the fertilization rate, and lipid profiles were measured. (3) Results: Initially, 86 patients were enrolled, with 65 completing the protocol (30 in the GF101 group and 34 in the CoQ10 group). There were no significant differences between the GF101 and CoQ10 groups in serum SOD (p = 0.626) and catalase levels (p = 0.061) over 12 weeks. However, within the GF101 group, a significant increase in serum catalase from baseline to 12 weeks was observed (p = 0.004). The non-inferiority analysis for IVF outcomes indicated risk differences in the clinical pregnancy rate, live birth rate, and miscarriage rate of −6.27% (95% CI: −30.77% to 18.22%), −1.18% (95% CI: −25.28% to 22.93%), and −13.49% (95% CI: −41.14% to 14.15%), respectively, demonstrating non-inferiority for the GF101 group. Furthermore, the GF101 group experienced significant reductions in total cholesterol (p = 0.006) and low-density lipoprotein (LDL) levels (p = 0.009) in intra-group comparisons, with both groups exhibiting comparable safe profiles. (4) Conclusions: GF101 may be non-inferior to CoQ10 in treating infertility in women and potentially offers additional benefits for women with dyslipidemia.
Hydrogen Sulfide Alleviates Oxidative Damage under Chilling Stress through Mitogen-Activated Protein Kinase in Tomato
Authors: Wu Guoxiu , Niu Xuxu , Chen Jiahui , Wu Changjiang , Li Yang , Li Yanman , Cui Dandan , He Xueying , Wang Fan , Li Shengli
Published: March 2024

Tomato is the vegetable with the largest greenhouse area in China, and low temperature is one of the main factors affecting tomato growth, yield, and quality. Hydrogen sulfide (H2S) plays an important role in regulating plant chilling tolerance, but its downstream cascade reaction and mechanism remain unclear. Mitogen-activated protein kinases (MAPK/MPKs) are closely related to a variety of signaling substances in stress signal transmission. However, whether H2S is related to the MPK cascade pathway in response to low-temperature stress is rarely reported. In this study, NaHS treatment significantly decreased the electrolyte leakage (EL), superoxide anion (O2−) production rate, and hydrogen peroxide (H2O2) content of seedlings at low temperatures. In addition, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were obviously increased; and the photochemical efficiency of PSII (Fv/Fm) was enhanced with treatment with NaHS, indicating that NaHS improved the seedlings’ cold tolerance by alleviating the degree of membrane lipid peroxidation and oxidative damage. However, H2S scavenger hypotaurine (HT) treatment showed the opposite effect. We found that H2S content, L-cysteine desulfhydrase (LCD) activity, and mRNA expression were increased by chilling stress but reduced by MPK inhibitor PD98059; PD98059 reversed the alleviating effect of H2S via increasing the EL and H2O2 contents. The expression levels of MPK1–MPK7 at low temperatures showed that SlMPK4 was significantly induced by exogenous NaHS and showed a trend of first increasing and then decreasing, while the expression level of SlMPK4 in HT-treated seedlings was lower than that of the control. After SlMPK4 was silenced by virus-induced gene silencing, the H2S-induced upregulation of C-repeat-Binding Factor (CBF1), inducer of CBF expression 1 (ICE1), respiratory burst oxidase homologs (RBOH1, RBOH2) at low temperatures disappeared, and tomato cold tolerance decreased. In conclusion, H2S improves the cold tolerance of tomato plants by increasing the activity of antioxidant enzymes and reducing reactive oxygen species (ROS) accumulation and membrane lipid peroxidation. MPK4 may act as a downstream signaling molecule in this process.
Ambient Vibration Analysis of Diversion Pipeline in Mount Changlong Pumped-Storage Power Station
Authors: Lian Jijian , Zuo Linrui , Wang Xiaoqun , Yu Lu
Published: March 2024

This study analyzes the ambient vibrations induced while running the Mount Changlong pumped-storage power station (PSPS). The ground vibration data of the power station during its operation were acquired with vibration sensors. Different units were selected and compared under working conditions, and the conclusions were as follows: (1) Ambient vibrations induced by the running of units constituted the primary source of vibration, and they attenuated as the distance increased. (2) The vibration acceleration under pumping conditions was larger than that under power generation conditions, and the ground vibration acceleration increased with an augmentation of the power. (3) The running of adjacent units generated mutual interference, and the types of units were different, which led to complex variations in the spectrum maps. (4) The vibration acceleration of the lower flat tunnel was prone to surpassing the standard when the number of units running together exceeded three.
MRAS Using Lyapunov Theory with Sliding Modes for a Fixed-Wing MAV
Authors: Espinoza-Fraire T. , Saenz Armando , Gandarilla Isaac , Giernacki Wojciech
Published: March 2024

This work applies an adaptive PD controller based on MRAS (Model Reference Adaptive System) using Lyapunov theory with sliding mode theory to a Fixed-wing MAV (Mini Aerial Vehicle). The objective is to design different adjustment mechanisms to obtain a robust adaptive control law in the presence of unknown perturbation due to wind gusts. Four adjustment mechanisms applied to an adaptive PD controller are compared. The adjustment mechanisms are Lyapunov theory, Lyapunov theory with first-order sliding mode, Lyapunov theory with second-order sliding mode, and Lyapunov theory with high-order sliding mode. Finally, after several simulations, a significant reduction and almost elimination of the unknown perturbations are presented with the addition of the sliding mode theory in the design of the adjustment mechanism for the adaptive PD controller.
Piezosurgery versus Reciprocating Saw: Qualitative Comparison of the Morphology of Cutting Surfaces in Ex Vivo Human Bone
Authors: Anesi Alexandre , Negrello Sara , Checchi Marta , Di Bartolomeo Mattia , Salvatori Roberta , Cavani Francesco , Palumbo Carla , Ferretti Marzia
Published: March 2024

The aim of this study was to morphologically evaluate the differences in the cutting surfaces of bone segments obtained by reciprocating saw (RS) and two piezosurgical devices (Piezosurgery Medical—PM—and Piezosurgery Plus—PP) in ex vivo human fibulae. The ultimate goal was to identify the presence of debris, scratches, and microcracks on the cutting surface that might affect bone healing, a key aspect in oral and maxillofacial surgery. Ten patients who underwent a microsurgical reconstruction of the mandible with a free fibula flap were enrolled. The fibula segments usually discarded after surgery were cut using RS, PM, and PP, obtaining transverse sections to analyze under an environmental scanning electron microscope to perform a histomorphological qualitative evaluation. Bone surfaces cut with the RS presented several scratches, and haversian canals were frequently filled with bone debris/chips. On the contrary, PM and PP devices produced smoother and sharper cutting surfaces, with lower production of bone debris/chips, preventing vascular spaces’ closure. Microcracks were found in both PM and PP cut specimens, and they could be associated with the triggering of bone remodeling, thus improving the formation of new bone, while their presence was rarely observable in RS cut samples. The use of piezosurgical devices showed superior performance, providing cleaner and smoother cutting surfaces that favor vascularization and bone remodeling; altogether, these processes could lead to accelerated bone healing, a fundamental goal in all surgical procedures that involve bone cutting.
Randomly Distributed Passive Seismic Source Reconstruction Record Waveform Rectification Based on Deep Learning
Authors: Zhao Binghui , Han Liguo , Zhang Pan , Feng Qiang , Ma Liyun
Published: March 2024

In passive seismic exploration, the number and location of underground sources are very random, and there may be few passive sources or an uneven spatial distribution. The random distribution of seismic sources can cause the virtual shot recordings to produce artifacts and coherent noise. These artifacts and coherent noise interfere with the valid information in the virtual shot record, making the virtual shot record a poorer presentation of subsurface information. In this paper, we utilize the powerful learning and data processing abilities of convolutional neural networks to process virtual shot recordings of sources in undesirable situations. We add an adaptive attention mechanism to the network so that it can automatically lock the positions that need special attention and processing in the virtual shot records. After testing, the trained network can eliminate coherent noise and artifacts and restore real reflected waves. Protecting valid signals means restoring valid signals with waveform anomalies to a reasonable shape.
Development of a Hardware-in-the-Loop Platform for a Teleoperation Flexibility Robotic System
Authors: Tran Duc Thien , Nguyen Tien Dat , Tran Minh Khiem , Ahn Kyoung Kwan
Published: March 2024

A control method for a cable-driven robot in a teleoperation system is proposed using the hardware-in-the-loop (HIL) simulation technique. The main components of the teleoperated robotic system are a haptic device, also called a delta robot, and a cable-driven hyper-redundant (CDHR) robot. The CDHR manipulator has higher flexibility and multiple degrees of freedom (DOF), and, therefore, its inverse kinematics are complex. For this reason, the Jacobian method is used in place of the conventional method to calculate the inverse kinematics. Moreover, the two robots constituting the telerobotic system are different in terms of their mechanical structures and workspaces. Therefore, the position mapping method is applied to ensure that the two workspaces are utilized together. However, a singularity area appears when the mapping parameter is adjusted to expand the workspace. Therefore, a haptic algorithm is proposed to prevent the robot from moving into the singularity region and generate force feedback at the end-effector of the haptic device to warn the operator. Because experimental verification of this control strategy is difficult, the HIL technique is used for demonstration in this study to ensure stability and safety before implementation of the method at the experiment scale. The CDHR robot is designed using SolidWorks 2021. Then, the Simscape model is used to simulate the telerobotic system. In addition, the protocol between the haptic device and the laptop is programmed using C/C++ language to facilitate communication with the CDHR robot in MATLAB Simulink 2022a. A few trials are conducted to evaluate and demonstrate the effectiveness of the proposed method.
Exploring the Cellular Interactions of Flavonoids with Similar Structures in Cells Overexpressing the 70 kDa Human Heat Shock Protein
Authors: Papa Garyfallia , Simos Yannis V. , Athinodorou Antrea-Maria , Tsamis Konstantinos I. , Peschos Dimitrios , Angelidis Charalampos , Pappas Periklis , Vezyraki Patra
Published: March 2024

Flavonoids share a common structural framework that serves as a hallmark indicative of their biological activity. In this study, we investigated the effects of two structurally similar flavonoids, fisetin and morin, through independent and combined in vitro assessments on embryonic mouse cells overexpressing the human 70 kDa heat shock protein (Hsp70) (Tg/Tg) and normal mouse fibroblast cell line (NIH/3T3). The primary objectives were to evaluate the biocompatibility and potential cytotoxicity of these flavonoids, along with assessing the cytoprotective role of Hsp70 in these cellular environments. To address these objectives, we conducted dose- and time-dependent cell survival tests. Additionally, we utilized flow cytometry to detect intracellular reactive oxygen species (ROS) production and to analyze apoptosis and the cell cycle. Throughout the experimental procedures, a notable observation was made: NIH/3T3 normal cells exhibited greater susceptibility compared to Tg/Tg cells when exposed to fisetin and morin. This difference in susceptibility is likely attributed to the robust cytoprotective effect of Hsp70 in Tg/Tg cells. Importantly, both cell lines demonstrated increased sensitivity to fisetin toxicity in comparison to morin, leading to significantly lower cell survival rates. These findings shed light on the differential responses of cell lines to flavonoid exposure, emphasizing the influence of Hsp70 and the distinct impact of fisetin and morin on cell viability.
Space Syntax at Expression of Science on User Flows in Open and Closed Spaces Aimed at Achieving the Sustainable Development Goal: A Review
Authors: Leite Paulo Wladinir da Luz , Almeida Silva Caliane Christie Oliveira de , Dal Moro Leila , Bodah Brian William , Mores Giana de Vargas , Piccinato Junior Dirceu , Engel Amanda , Santosh M. , Neckel Alcindo
Published: March 2024

Space syntax is utilized to model flows through open and closed built environments, which enables project innovation by rethinking the design of spaces on a global scale with better flow quality. Therefore, studies focused on spatial syntax, when related to comprehensive flows in open and closed spaces, provide a holistic and valuable understanding of the dynamics of user flows in the urban environment from a perspective centered on the United Nation’s Sustainable Development Goal (SDG) 11. This goal requires urban planners and designers to use approaches that support future decisions focused on urban design and planning. The object of investigation of this bibliographic study consists of an approach to representing space syntax in open spaces and closed spaces on a global scale. This study aims to analyze the concepts of pedestrian flows (open and closed) by a space syntax-based bibliographical approach on a global scale, demonstrating the capability of improvements in SDG 11 as applied to the architecture of sustainable flows. Methodologically, bibliographic searches were carried out using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) method in databases linked to Scopus and ScienceDirect, focusing on space syntax in relation to the following terms: “open spaces” and “closed spaces”. Using the PRISMA method, 1986 manuscripts dealing with the term “open spaces” and 454 manuscripts dealing with the term “closed spaces” were identified, with the manuscripts most relevant to the topic being selected, followed by a frequency analysis based on a Content Analysis Method (CAM) to identify words with a degree of similarity, related to “spatial syntax: flow in urban environments” and “spatial syntax in closed built systems” in relation to the SDG 11. The results demonstrate the relevance of seven manuscripts in open spaces and four manuscripts in closed spaces related to space syntax. Frequency analysis identified open spaces and produced terms with frequencies such as space = 79 and shape = 46, showing a higher frequency in flows. In closed spaces, the literature has shown that the central term corresponds to space = 79 and flow = 76, making it possible to evaluate flows in circulation areas within the built environment. This study allows a better understanding of flows, highlighting the importance of the urban architecture in the functionality of user flows in sustainable environments, which is capable of contributing to the SDG 11, in the interface of architectural projects on a global scale.
Effects of Different Therapeutic Approaches on Redox Balance in Psoriatic Patients
Authors: Medovic Marija V. , Milicic Vesna M. , Nikolic Ana B. Ravic , Ristic Gordana J. , Medovic Rasa H. , Nikolic Marina R. , Stojanovic Aleksandra Z. , Bolevich Sergey B. , Bondarchuk Natalia G. , Gorbunov Alexander A. , Mitrovic Slobodanka L. , Jakovljevic Vladimir Lj. , Srejovic Ivan M.
Published: March 2024

Given that oxidative stress represents an important etiological factor in the pathogenesis of psoriasis, the aim of this study was to assess the effects of different therapeutic approaches, methotrexate, secukinumab, and ustekinumab on systemic oxidative stress biomarkers in psoriatic patients. This study involved 78 psoriatic patients, divided into the group treated with methotrexate (23 patients), secukinumab (28 patients), and ustekinumab (27 patients), and 15 healthy controls. Oxidative stress biomarkers (index of lipid peroxidation measured as TBARS, nitrites (NO2−), superoxide anion radical (O2−), and hydrogen peroxide (H2O2)) and antioxidative defense system (superoxide dismutase (SOD) activity, catalase (CAT) activity, and reduced glutathione (GSH)) were determined spectrophotometrically from the blood before the initiation of therapy in 16th, 28th, and 52nd week. O2− and SOD showed the most prominent changes comparing the psoriatic patients and healthy controls. CAT activity was significantly lower in psoriatic patients, and methotrexate induced a further decline in CAT activity. Ustekinumab induced a significant increase in GSH level after 52 weeks of treatment, while methotrexate reduced GSH. All applied therapeutic options induced a reduction in PASI, BSA, DLQI, and EARP. Biological drugs exert more pronounced antioxidant effects compared to methotrexate, which is most clearly observed in the values of O2− and SOD.
CLCA2: A Potential Guardian against Premature Senescence and Skin Aging
Authors: Guerrero-Navarro Lena , Martic Ines , Ploner Christian , Jansen-Dürr Pidder , Cavinato Maria
Published: March 2024

Cellular senescence, a state of irreversible growth arrest, is implicated in various age-related pathologies, including skin aging. In this study, we investigated the role of CLCA2, a calcium-activated chloride channel accessory protein, in cellular senescence and its implications for skin aging. Utilizing UVB and Nutlin3a-induced senescence models, we observed the upregulation of CLCA2 at both transcriptomic and proteomic levels, suggesting its involvement in senescence pathways. Further analysis revealed that the depletion of CLCA2 led to accelerated senescence onset, characterized by classic senescence markers and a unique secretome profile. In 3D skin equivalent models, SEs constructed with CLCA2 knockdown fibroblasts exhibited features reminiscent of aged skin, underscoring the importance of CLCA2 in maintaining skin homeostasis. Our findings highlight CLCA2 as a novel regulator of cellular senescence and its potential implications for skin aging mechanisms.
Simple, Visual, Point-of-Care SARS-CoV-2 Detection Incorporating Recombinase Polymerase Amplification and Target DNA–Protein Crosslinking Enhanced Chemiluminescence
Authors: Chen Hui , Zhuang Zhiyuan , Xu Naihan , Feng Ying , Fang Kaixin , Tan Chunyan , Tan Ying
Published: March 2024

The ongoing COVID-19 pandemic, driven by persistent SARS-CoV-2 transmission, threatens human health worldwide, underscoring the urgent need for an efficient, low-cost, rapid SARS-CoV-2 detection method. Herein, we developed a point-of-care SARS-CoV-2 detection method incorporating recombinase polymerase amplification (RPA) and DNA–protein crosslinking chemiluminescence (DPCL) (RPADPCL). RPADPCL involves the crosslinking of biotinylated double-stranded RPA DNA products with horseradish peroxidase (HRP)-labeled streptavidin (SA-HRP). Modified products are captured using SA-labeled magnetic beads, and then analyzed using a chemiluminescence detector and smartphone after the addition of a chemiluminescent substrate. Under optimal conditions, the RPADPCL limit of detection (LOD) was observed to be 6 copies (within the linear detection range of 1–300 copies) for a plasmid containing the SARS-CoV-2 N gene and 15 copies (within the linear range of 10–500 copies) for in vitro transcribed (IVT) SARS-CoV-2 RNA. The proposed method is convenient, specific, visually intuitive, easy to use, and does not require external excitation. The effective RPADPCL detection of SARS-CoV-2 in complex matrix systems was verified by testing simulated clinical samples containing 10% human saliva or a virus transfer medium (VTM) spiked with a plasmid containing a SARS-CoV-2 N gene sequence or SARS-CoV-2 IVT RNA. Consequently, this method has great potential for detecting targets in clinical samples.
The Left Amygdala and Right Frontoparietal Cortex Support Emotional Adaptation Aftereffects
Authors: Su Xinqi , Fu Ruilin , Li Huiling , Jiang Nan , Li Aqian , Yang Jingyu , Mei Leilei
Published: March 2024

Adaptation aftereffects—in which prolonged prior experience (adaptation) can bias the subsequent judgment of ambiguous stimuli—are a ubiquitous phenomenon. Numerous studies have found behaviorally stable adaptation aftereffects in a variety of areas. However, it is unclear which brain regions are responsible for this function, particularly in the case of high-level emotional adaptation aftereffects. To address this question, the present study used fMRI technology to investigate the neural mechanism of emotional adaptation aftereffects. Consistent with previous studies, we observed typical emotional adaptation effects in behavior. Specifically, for the same morphed facial images, participants perceived increased sadness after adapting to a happy facial image and increased happiness after adapting to a sad facial image. More crucially, by contrasting neural responses to ambiguous morphed facial images (i.e., facial images of intermediate morph levels) following adaptation to happy and sad expressions, we demonstrated a neural mechanism of emotional aftereffects supported by the left amygdala/insula, right angular gyrus, and right inferior frontal gyrus. These results suggest that the aftereffects of emotional adaptation are supported not only by brain regions subserving emotional processing but also by those subserving cognitive control.
Impacts of User Personality Traits on Their Contributions in Idea Implementation: A Moderated Mediation Model
Authors: Mi Xuejiao , Zhang Huiying , Qu Fei
Published: March 2024

In the realm of open innovation, users have emerged as a significant external source of innovation that enterprises cannot afford to overlook. Implemented ideas play a pivotal role in driving the iterative innovation of products within enterprises. However, the existing literature still lacks an exploration of specific impact mechanisms on contributions in idea implementation. This study presents a model that delineates the impact of user personality traits on idea implementation contributions, drawing upon theories such as personality trait theory, user engagement perspective, and trait activation theory. Empirical research was carried out by utilizing user data obtained from the Chinese high-tech company Xiaomi’s MIUI community. Personality trait indicators were developed through the application of text mining and machine learning techniques. To evaluate the models, a negative binomial regression model, which is well-suited for handling discrete data, was employed. The findings of this study indicate that user openness and conscientiousness positively influence their idea implementation contribution, whereas neuroticism has a negative impact on implementation contribution. Additionally, it is observed that user engagement plays a partial mediating role in the relationship between openness, conscientiousness, neuroticism, and idea implementation contribution. Community incentives can positively moderate the impact of user engagement on the relationship between conscientious personality and idea implementation contribution. This study expands the analysis of the impact mechanism of user idea implementation contributions, which has important theoretical guidance and practical implications for accurately identifying leading users in open innovation communities and enhancing user innovation contributions.
The Hidden Impact of ADHD Symptoms in Preschool Children with Autism: Is There a Link to Somatic and Sleep Disorders?
Authors: Esposito Dario , Bernardi Katerina , Belli Arianna , Gasparri Valentina , Romano Sara , Terenzi Letizia , Zanatta Maria Elena , Iannotti Sara , Ferrara Mauro
Published: March 2024

Neurodevelopmental disorders, such as autism spectrum disorder (ASD) and attention deficit hyperactivity disorder (ADHD), do often present in comorbidity among them and with other medical conditions, including sleep and gastrointestinal (GI) disorders and somatic complaints. An anonymous online survey based on standardized questionnaires (SDSC, KL-ASD, APSI, ROME V CRITERIA, CPRS, CBCL) was completed by the parents of 46 preschoolers diagnosed with ASD. A high prevalence (47%) of ADHD symptoms in this population was found, surpassing previous estimates. Sleep disturbances, especially difficulties in initiating and maintaining sleep and sleep–wake transition, are more pronounced in ASD patients with comorbid ADHD. Additionally, in patients over 4 years old, there is a high prevalence of functional GI symptoms (Rome V criteria: 38%). Parental stress is significantly associated with ADHD symptoms, sleep disorders, and GI symptoms. Comprehensive assessments considering both core symptoms of neurodevelopmental disorders and associated comorbidities are crucial for more effective treatment strategies and improved wellbeing in affected individuals and their families.
Effect of Banking Time Intervention on Child–Teacher Relationships and Problem Behaviors in China: A Multiple Baseline Design
Authors: Zhang Zedong , Wang Ye
Published: March 2024

A positive child–teacher relationship is a crucial means of addressing problem behaviors in young children. In recent years, there has been an increase in factors triggering problem behaviors in young children. It is particularly important to employ universally applicable and scientifically effective strategies to improve child behavior. Banking Time, as an emerging variant of play therapy, aims to enhance child behavior by establishing an intimate child–teacher relationship. This study conducted a multiple-baseline experiment involving eight four-year-old children and their teachers from China, exploring the effectiveness of Banking Time in enhancing child–teacher relationships and subsequently improving child behavior from dual perspectives, utilizing tools such as the Student–Teacher Relationship Scale and Conners’ Comprehensive Behavior Rating Scales-Teacher Assessment Report. Visual analysis and statistical analysis results indicate a strong positive impact of Banking Time on child–teacher relationships and a slight inhibitory effect on child problem behaviors. The implementation of Banking Time provides valuable insights into specific paths and strategies for promoting teachers’ professional development.
Examining the Antecedents of Novice STEM Teachers’ Job Satisfaction: The Roles of Personality Traits, Perceived Social Support, and Work Engagement
Authors: Wang Zhaochun , Jiang Haozhe , Jin Wu , Jiang Jin , Liu Jiahong , Guan Jia , Liu Yangxi , Bin Enlin
Published: March 2024

It is reported that teachers’ satisfaction levels are lower and stress levels are higher than other professional groups in many countries. This is especially true for science, technology, engineering, and mathematics (STEM) teachers. Considering job satisfaction has a direct impact on turnover intention, low satisfaction levels have led to high turnover rates and thus exacerbated the shortages of STEM teachers. Hence, there is an urgent need to explore the antecedents of novice STEM teachers’ job satisfaction. This study proposes a new model to show how novice STEM teachers’ personality traits and perceived social support influence their job satisfaction. A total of 2592 novice STEM teachers in eighteen randomly selected high schools in China were recruited. Data were analyzed using the structural equation modeling approach. The results illustrated that most novice STEM teachers had low levels of job satisfaction. Novice STEM teachers’ personality traits could directly and indirectly impact their job satisfaction. Meanwhile, novice STEM teachers’ job satisfaction was also directly influenced by work engagement and indirectly influenced by their perceived social support. Our findings theoretically contribute to the understanding of the determinants of STEM teachers’ job satisfaction and have important practical implications.
The Impact of Dating Applications on Adolescent Development: A Psychological Perspective
Authors: Cerniglia Luca
Published: March 2024

The genesis of dating applications can be traced back to the early days of the internet, where websites served as the primary medium for digital dating [...]
Investigation into the Bearing Capacity and Mechanics Behavior of the Diaphragm Connection Form of a Utility Tunnel
Authors: Dai Yongxing , Zeng Yi , Shi Bolun , Li Hongbo
Published: March 2024

To investigate the impact of various compartment partition plate connection methods within a shield utility tunnel on the mechanics behavior of the connecting nodes and the overall structural integrity, this study examines and simulates three distinct connection approaches in a laboratory. These approaches include a steel corbel and rear expansion anchor bolt connection, an embedded part and steel corbel welding connection, and a reinforced concrete corbel connection. The objective in selecting the above three connection methods was to gain insights into how they influence the mechanical properties of the connections and the tunnel structure itself. The failure criteria of the structure dictate that neither the steel bar nor the steel plate should exceed their respective yield strength. Furthermore, the concrete damage zone surrounding the anchor should not exhibit any connectivity. The findings of our study indicate that: (1) The weak link in the steel truss-rear expansion anchor bolt connection scheme is centered within the connection section. With six rear expansion anchor bolts, the load capacity reached 180 kN. Conversely, when employing nine rear expansion anchor bolts, the reduced spacing between the bolts led to premature concrete breakage, decreasing the bearing capacity to 170 kN. (2) Arranging the six anchor bolts into two rows and three columns enhanced the load-bearing capacity, yet one must be cautious to prevent damage from incorrect bolt spacing. According to the conditions outlined in this study, the ideal bolt spacing fell within the range from 66.7 mm to 100 mm. Additionally, it is worth noting that the bolt deformation was concentrated within 5 cm and 6 cm around the bolt. (3) The connection scheme of the embedded part and steel corbel demonstrated impressive load-bearing capabilities, showing the ability to withstand a load of 220 kN within the elastic stage. Notably, the deformation of the anchor bar was concentrated primarily within a 5 cm radius around the corbel. (4) In the reinforced concrete corbel connection scheme, the load-bearing capacity reached 240 kN. The key factor influencing this capacity was the presence of cracks. Initially, these cracks appeared symmetrically on both sides of the corbel, and gradually extended to the width and height of the corbel structure.
Flexural Behavior of Alkali-Activated Ultra-High-Performance Geopolymer Concrete Beams
Authors: Su Jie , Tan Jiandong , Li Kai , Fang Zhi
Published: March 2024

Ultra-high-performance geopolymer concrete (UHPGC) emerges as a sustainable and cost-effective alternative to Portland cement-based UHPC, offering similar mechanical properties while significantly reducing carbon footprint and energy consumption. Research on UHPGC components is extremely scarce. This study focuses on the flexural and crack behavior of UHPGC beams with different steel fiber contents and longitudinal reinforcement ratios. Five UHPGC beams were tested under four-point bending. The test results were evaluated in terms of the failure mode, load–deflection relationship, flexural capacity, ductility, average crack spacing, and short-term flexural stiffness. The results show that all the UHPGC beams failed due to crack localization. Increases in the reinforcement ratio and steel fiber content had favorable effects on the flexural capacity and flexural stiffness. When the reinforcement ratio increased from 1.18% to 2.32%, the flexural capacity and flexural stiffness increased by 60.5% and 12.3%, respectively. As the steel fiber content increased from 1.5% to 2.5%, the flexural capacity and flexural stiffness increased by 4.7% and 4.4%, respectively. Furthermore, the flexural capacity, flexural stiffness, and crack spacing of the UHPGC beams were evaluated using existing methods. The results indicate that the existing methods can effectively predict flexural capacity and flexural stiffness in UHPGC beams but overestimate crack spacing. This study will provide a reference for the structural design of UHPGC.
Unlocking the Potential of Digital Twins in Construction: A Systematic and Quantitative Review Using Text Mining
Authors: Park Jisoo , Lee Jae-Kang , Son Min-Jae , Yu Chaeyeon , Lee Jaesung , Kim Sungjin
Published: March 2024

The construction industry has been trying to enhance the level of digitalization and autonomy by adopting various communication and information technologies (ICT), e.g., augmented reality (AR), virtual reality (VR), robotics, drones, or building information modeling (BIM). However, improvement of the safety and productivity in their domains is still a struggle. One of the main reasons for failing to accelerate their digital transformation is ignoring the deep understanding of the concept of digital twin, its usage, and the potential benefits of digital twins in the construction industry. Therefore, this paper investigated the impacts and potentials of digital twins on the construction industry through a quantitative systematic review assisted by the text mining method. The study presented the potential usability of digital twins, leading and core technologies, and applications, revealing their benefits and potential for optimizing project planning, execution, and management process. Through this comprehensive literature review, this study elucidated the distinctive features, advantages, and immense potential that digital twins bring to the construction field. The findings highlight the transformative impact of digital twins, providing critical insights for their broader adoption and groundbreaking applications in the industry. By addressing the challenges of adopting this technology, the article provided valuable insights for advancing research and the broad implementation of digital twins in the sector.
Influence of Balcony Thermal Bridges on Energy Efficiency of Dwellings in a Warm Semi-Arid Dry Mediterranean Climate
Authors: Pérez-Carramiñana Carlos , de la Morena-Marqués Aurelio , González-Avilés Ángel Benigno , Castilla Nuria , Galiano-Garrigós Antonio
Published: March 2024

Thermal bridges significantly influence the energy performance of buildings. However, their impact varies depending on the type of thermal bridge, climate conditions, construction methodologies, and geometric characteristics of the building. On the Spanish Mediterranean coast, buildings with large balconies are predominant. Nevertheless, the Spanish energy efficiency regulations do not adequately specify the thermal bridges at the junctions of balconies with facades, leading to a lack of consideration for their influence in the majority of architectural projects. The objective of this study is to qualitatively and quantitatively assess the impact of such thermal bridges on the energy efficiency of buildings in a dry Mediterranean climate (BShs) within a warm semi-arid climate (BSh). As a case study, the influence of this thermal bridge is analyzed in two residential buildings located on the Mediterranean coast of southeastern Spain. The study also examines the modification of various construction parameters of this thermal bridge and determines the optimal design parameters to reduce its thermal transmittance. The results demonstrate that the energy needs caused by thermal bridges account for approximately 40% of the total annual energy needs of the studied residential buildings. Balcony thermal bridges account for 25% to 40% of the energy needs caused by all thermal bridges. The lack of differentiation in Spanish standards between balcony–facade and facade–slab edge junctions causes an imprecision in calculations equivalent to 12% of the total annual energy needs of dwellings. The novelty of this research lies in highlighting that current regulations and calculation programs need improvement to better characterize balcony thermal bridges and enhance the accuracy of building energy efficiency calculations.