bluuebunny/crossref_metadata_2025 huggingface dataset:
The Inhibitory Role of miR-486-5p on CSC Phenotype Has Diagnostic and Prognostic Potential in Colorectal Cancer
Authors: Pisano Andrea , Griñan-Lison Carmen , Farace Cristiano , Fiorito Giovanni , Fenu Grazia , Jiménez Gema , Scognamillo Fabrizio , Peña-Martin Jesùs , Naccarati Alessio , Pröll Johannes , Atzmüller Sabine , Pardini Barbara , Attene Federico , Ibba Gabriele , Solinas Maria Giuliana , Bernhard David , Marchal Juan Antonio , Madeddu Roberto
Published: November 2020

Colorectal cancer (CRC) is the third most frequent cancer worldwide and the second cause of cancer deaths. Increasing evidences supports the idea that the poor prognosis of patients is related to the presence of cancer stem cells (CSCs), a cell population able to drive cancer recurrence and metastasis. The deregulation of microRNAs (miRNAs) plays a role in the formation of CSC. We investigated the role of hsa-miR-486-5p (miR-486-5p) in CRC, CSCs, and metastasis, in order to reach a better understanding of the biomolecular and epigenetic mechanisms mir-486-5p-related. The expression of miR-486-5p was investigated in three different matrices from CRC patients and controls and in CSCs obtained from the CRC cell lines HCT-116, HT-29, and T-84. In the human study, miR-486-5p was up-regulated in serum and stool of CRC patients in comparison with healthy controls but down-regulated in tumor tissue when compared with normal mucosa. miR-486-5p was also down-regulated in the sera of metastatic patients. In vitro, miR-486-5p was down-regulated in CSC models and it induced an inhibitory effect on stem factors and oncogenes in the main pathways of CSCs. Our results provide a step forward in understanding the role of mir-486-5p in CRC and CSC, and suggest that further studies are needed to investigate its diagnostic and prognostic power, possibly in combination with other biomarkers.
Opportunities for Single-Cell Sequencing Technologies and Data Science
Authors: Mustachio Lisa Maria , Roszik Jason
Published: November 2020

This Special Issue on “Single-cell Data Science” aims to highlight recent advances in the area of single-cell sequencing technologies and data analytics [...]
The Anti-Cancer Properties of the HIV Protease Inhibitor Nelfinavir
Authors: Subeha Mahbuba R. , Telleria Carlos M.
Published: November 2020

Traditional cancer treatments may lose efficacy following the emergence of novel mutations or the development of chemoradiotherapy resistance. Late diagnosis, high-cost of treatment, and the requirement of highly efficient infrastructure to dispense cancer therapies hinder the availability of adequate treatment in low-income and resource-limited settings. Repositioning approved drugs as cancer therapeutics may reduce the cost and timeline for novel drug development and expedite the availability of newer, efficacious options for patients in need. Nelfinavir is a human immunodeficiency virus (HIV) protease inhibitor that has been approved and is extensively used as an anti-infective agent to treat acquired immunodeficiency syndrome (AIDS). Yet nelfinavir has also shown anti-cancer effects in in vitro and in vivo studies. The anti-cancer mechanism of nelfinavir includes modulation of different cellular conditions, such as unfolded protein response, cell cycle, apoptosis, autophagy, the proteasome pathway, oxidative stress, the tumor microenvironment, and multidrug efflux pumps. Multiple clinical trials indicated tolerable and reversible toxicities during nelfinavir treatment in cancer patients, either as a monotherapy or in combination with chemo- or radiotherapy. Since orally available nelfinavir has been a safe drug of choice for both adult and pediatric HIV-infected patients for over two decades, exploiting its anti-cancer off-target effects will enable fast-tracking this newer option into the existing repertoire of cancer chemotherapeutics.
A Detailed Flow Cytometric Analysis of Immune Activity Profiles in Molecular Subtypes of Colorectal Cancer
Authors: Li Xingru , Ling Agnes , Kellgren Therese G. , Lundholm Marie , Löfgren-Burström Anna , Zingmark Carl , Rutegård Martin , Ljuslinder Ingrid , Palmqvist Richard , Edin Sofia
Published: November 2020

The local anti-tumour immune response has important prognostic value in colorectal cancer (CRC). In the era of immunotherapy, a better understanding of the immune response in molecular subgroups of CRC may lead to significant advances in personalised medicine. On this note, microsatellite instable (MSI) tumours have been characterised by increased immune infiltration, suggesting MSI as a marker for immune inhibitor checkpoint therapy. Here, we used flow cytometry to perform a comprehensive analysis of immune activity profiles in tumour tissues, adjacent non-malignant tissues and blood, from a cohort of 69 CRC patients. We found several signs of immune suppression in tumours compared to adjacent non-malignant tissues, including T cells more often expressing the immune checkpoint molecules programmed cell death protein (PD-1) and cytotoxic T lymphocyte-associated protein 4 (CTLA-4). We further analysed immune cell infiltration in molecular subgroups of CRC. MSI tumours were indeed found to be associated with increased immune infiltration, including increased fractions of PD-1+ T cells. No correlation was, however, found between MSI and the fraction of CTLA-4+ T cells. Interestingly, within the group of patients with microsatellite stable (MSS) tumours, some also presented with increased immune infiltration, including comparably high portions of PD-1+ T cells, but also CTLA-4+ T cells. Furthermore, no correlation was found between PD-1+ and CTLA-4+ T cells, suggesting that different tumours may, to some extent, be regulated by different immune checkpoints. We further evaluated the distribution of immune activity profiles in the consensus molecular subtypes of CRC. In conclusion, our findings suggest that different immune checkpoint inhibitors may be beneficial for selected CRC patients irrespective of MSI status. Improved predictive tools are required to identify these patients.
Wnt/β-Catenin Target Genes in Colon Cancer Metastasis: The Special Case of L1CAM
Authors: Cheriyamundath Sanith , Ben-Ze’ev Avri
Published: November 2020

Cell adhesion to neighboring cells is a fundamental biological process in multicellular organisms that is required for tissue morphogenesis. A tight coordination between cell–cell adhesion, signaling, and gene expression is a characteristic feature of normal tissues. Changes, and often disruption of this coordination, are common during invasive and metastatic cancer development. The Wnt/β-catenin signaling pathway is an excellent model for studying the role of adhesion-mediated signaling in colorectal cancer (CRC) invasion and metastasis, because β-catenin has a dual role in the cell; it is a major adhesion linker of cadherin transmembrane receptors to the cytoskeleton and, in addition, it is also a key transducer of Wnt signaling to the nucleus, where it acts as a co-transcriptional activator of Wnt target genes. Hyperactivation of Wnt/β-catenin signaling is a common feature in the majority of CRC patients. We found that the neural cell adhesion receptor L1CAM (L1) is a target gene of β-catenin signaling and is induced in carcinoma cells of CRC patients, where it plays an important role in CRC metastasis. In this review, we will discuss studies on β-catenin target genes activated during CRC development (in particular, L1), the signaling pathways affected by L1, and the role of downstream target genes activated by L1 overexpression, especially those that are also part of the intestinal stem cell gene signature. As intestinal stem cells are highly regulated by Wnt signaling and are believed to also play major roles in CRC progression, unravelling the mechanisms underlying the regulation of these genes will shed light on both normal intestinal homeostasis and the development of invasive and metastatic CRC.
Complications after CD19+ CAR T-Cell Therapy
Authors: Penack Olaf , Koenecke Christian
Published: November 2020

Clinical trials demonstrated that CD19+ chimeric antigen receptor (CAR) T-cells can be highly effective against a number of malignancies. However, the complete risk profile of CAR T-cells could not be defined in the initial trials. Currently, there is emerging evidence derived from post approval studies in CD19+ CAR T-cells demonstrating both short-term and medium-term effects, which were unknown at the time of regulatory approval. Here, we review the incidence and the current management of CD19+ CAR T-cell complications. We highlight frequently occurring events, such as cytokine release syndrome, immune effector cell-associated neurotoxicity syndrome, cardiotoxicity, pulmonary toxicity, metabolic complications, secondary macrophage-activation syndrome, and prolonged cytopenia. Furthermore, we present evidence supporting the hypothesis that CAR T-cell-mediated toxicities can involve any other organ system and we discuss the potential risk of long-term complications. Finally, we discuss recent pre-clinical and clinical data shedding new light on the pathophysiology of CAR T-cell-related complications.
Catalysis with Silver: From Complexes and Nanoparticles to MORALs and Single-Atom Catalysts
Authors: Pagliaro Mario , Della Pina Cristina , Mauriello Francesco , Ciriminna Rosaria
Published: November 2020

Silver catalysis has a rich and versatile chemistry now expanding from processes mediated by silver complexes and silver nanoparticles to transformations catalyzed by silver metal organic alloys and single-atom catalysts. Focusing on selected recent advances, we identify the key advantages offered by these highly selective heterogeneous catalysts. We conclude by offering seven research and educational guidelines aimed at further progressing the field of new generation silver-based catalytic materials.
Ribosomal Protein L10: From Function to Dysfunction
Authors: Pollutri Daniela , Penzo Marianna
Published: November 2020

Eukaryotic cytoplasmic ribosomes are highly structured macromolecular complexes made up of four different ribosomal RNAs (rRNAs) and 80 ribosomal proteins (RPs), which play a central role in the decoding of genetic code for the synthesis of new proteins. Over the past 25 years, studies on yeast and human models have made it possible to identify RPL10 (ribosomal protein L10 gene), which is a constituent of the large subunit of the ribosome, as an important player in the final stages of ribosome biogenesis and in ribosome function. Here, we reviewed the literature to give an overview of the role of RPL10 in physiologic and pathologic processes, including inherited disease and cancer.
Tackling Childhood Stunting in the Eastern Mediterranean Region in the Context of COVID-19
Authors: Jawaldeh Ayoub Al , Doggui Radhouene , Borghi Elaine , Aguenaou Hassan , Ammari Laila El , Abul-Fadl Azza , McColl Karen
Published: November 2020

Over 20 million children under 5 years old in the WHO Eastern Mediterranean Region have stunted growth, as a result of chronic malnutrition, with damaging long-term consequences for individuals and societies. This review extracted and analyzed data from the UNICEF, WHO and the World Bank malnutrition estimates to present an overall picture of childhood stunting in the region. The number of children under 5 in the region who are affected by stunting has dropped from 24.5 million (40%) in 1990 to 20.6 million (24.2%) in 2019. The reduction rate since the 2012 baseline is only about two fifths of that required and much more rapid progress will be needed to reach the internationally agreed targets by 2025 and 2030. Prevalence is highest in low-income countries and those with a lower Human Development Index. The COVID-19 pandemic threatens to undermine efforts to reduce stunting, through its impact on access and affordability of safe and nutritious foods and access to important health services. Priority areas for action to tackle stunting as part of a comprehensive, multisectoral nutrition strategy are proposed. In light of the threat that COVID-19 will exacerbate the already heavy burden of malnutrition in the Eastern Mediterranean Region, implementation of such strategies is more important than ever.
A Review on Anodizing of Aerospace Aluminum Alloys for Corrosion Protection
Authors: Paz Martínez-Viademonte Mariana , Abrahami Shoshan T. , Hack Theodor , Burchardt Malte , Terryn Herman
Published: November 2020

Aluminum alloys used for aerospace applications provide good strength to weight ratio at a reasonable cost but exhibit only limited corrosion resistance. Therefore, a durable and effective corrosion protection system is required to fulfil structural integrity. Typically, an aerospace corrosion protection system consists of a multi-layered scheme employing an anodic oxide with good barrier properties and a porous surface, a corrosion inhibited organic primer, and an organic topcoat. The present review covers published research on the anodic oxide protection layer principles and requirements for aerospace application, the effect of the anodizing process parameters, as well as the importance of process steps taking place before and after anodizing. Moreover, the challenges of chromic acid anodizing (CAA) substitution are discussed and tartaric-sulfuric acid anodizing (TSA) is especially highlighted among the environmentally friendly alternatives.
Physicochemical and Mechanical Properties of Blow Spun Nanofibrous Prostheses Modified with Acrylic Acid and REDV Peptide
Authors: Butruk-Raszeja Beata A. , Kuźmińska Aleksandra , Wojasiński Michał , Piotrowska Zuzanna
Published: November 2020

The paper presents a method of modifying the inner surface of nanofibrous vascular prostheses. The modification process involves two steps: introducing a hydrophilic linker, followed by a peptide containing the arginine-glutamic acid-aspartic acid-valine (REDV) sequence. The influence of the process parameters (reaction time, temperature, initiator concentration) on morphology and the distribution of fiber diameters were examined. For selected optimal parameters, the prostheses were modified in the flow system. Modifications along the entire length of the prosthesis were confirmed—the inlet and the outlet areas showed no significant (p > 0.05) differences in the value of the contact angle and the analyzed morphological parameters. The basic physicochemical and mechanical properties of modified prostheses were analyzed. The study showed that REDV-modified prosthesis has an average fiber diameter of 318 ± 99 nm, the average pore size of 3.0 ± 1.6 μm, the porosity of 48.4 ± 8.6% and Young’s modulus of 4.0 ± 0.4 MPa. The internal diameter of prostheses remains unchained and amounts to 3 mm. Such modified prostheses can reduce the risk of blood coagulation by increasing the surface’s wettability and, most of all, by introducing endothelial cell-selective peptide. As an effect, the proposed surfaces could recruit endothelial progenitor cells directly from the bloodstream and promote the endothelium formation after implantation.
Termination Effects in Aluminosilicate and Aluminogermanate Imogolite Nanotubes: A Density Functional Theory Study
Authors: Poli Emiliano , Elliott Joshua D. , Chai Ziwei , Teobaldi Gilberto
Published: November 2020

We investigate termination effects in aluminosilicate (AlSi) and aluminogermanate (AlGe) imogolite nanotubes (NTs) by means of semi-local and range-corrected hybrid Density Functional Theory (DFT) simulations. Following screening and identification of the smallest finite model capable of accommodating full relaxation of the NT terminations around an otherwise geometrically and electrostatically unperturbed core region, we quantify and discuss the effects of physical truncation on the structure, relative energy, electrostatics and electronic properties of differently terminated, finite-size models of the NTs. In addition to composition-dependent changes in the valence (VB) and conduction band (CB) edges and resultant band gap (BG), the DFT simulations uncover longitudinal band bending and separation in the finite AlSi and AlGe models. Depending on the given termination of the NTs, such longitudinal effects manifest in conjunction with the radial band separation typical of fully periodic AlSi and AlGe NTs. The strong composition dependence of the longitudinal and radial band bending in AlSi and AlGe NTs suggests different mechanisms for the generation, relaxation and separation of photo-generated holes in AlSi and AlGe NTs, inviting further research in the untapped potential of imogolite compositional and structural flexibility for photo-catalytic applications.
Effect of Organic and Conventional Systems Used to Grow Pecan Trees on Diversity of Soil Microbiota
Authors: Cabrera-Rodríguez Alejandra , Nava-Reyna Erika , Trejo-Calzada Ricardo , García-De la Peña Cristina , Arreola-Ávila Jesús G. , Collavino Mónica M. , Vaca-Paniagua Felipe , Díaz-Velásquez Clara , Constante-García Vicenta
Published: November 2020

Agronomic management modifies the soil bacterial communities and may alter the carbon fractions. Here, we identify differences in several chemical and biological soil variables, as well as bacterial composition between organic (Org) and conventional (Conv) agronomic management in pecan (Carya illinoinensis) orchards located in Coahuila, Mexico. The analyzed variables were pH, N, P, K, soil organic matter, organic matter quality, soil organic carbon, C/N ratio, carbon fractions, microbial biomass carbon, easily extractable Glomalin, colony-forming units, CO2 emissions, and the enzyme activity. The DNA of soil bacteria was extracted, amplified (V3-V4 16S rRNA), and sequenced using Illumina. To compare variables between agronomic managements, t tests were used. Sequences were analyzed in QIIME (Quantitative Insights Into Microbial Ecology). A canonical correspondence analysis (CCA) was used to observe associations between the ten most abundant phyla and soil variables in both types of agronomic managements. In Org management, variables related to the capture of recalcitrant carbon compounds were significant, and there was a greater diversity of bacterial communities capable of promoting organic carbon sequestration. In Conv management, variables related to the increase in carbon mineralization, as well as the enzymatic activity related to the metabolism of labile compounds, were significant. The CCA suggested a separation between phyla associated with some variables. Agronomic management impacted soil chemical and biological parameters related to carbon dynamics, including bacterial communities associated with carbon sequestration. Further research is still necessary to understand the plasticity of some bacterial communities, as well as the soil–plant dynamics.
Periodontal Health and Systemic Conditions
Authors: Lim Glendale , Janu Upasna , Chiou Lan-Lin , Gandhi Kaveri Kranti , Palomo Leena , John Vanchit
Published: November 2020

According to the new classification proposed by the recent 2017 World Workshop on Periodontal and Peri-implant Diseases and Conditions, periodontitis, necrotizing periodontal diseases, periodontitis as a manifestation of systemic diseases, and systemic diseases or conditions affecting the periodontal supporting tissues, are considered as separate entities. Scientific evidence has demonstrated that periodontal diseases are not just simple bacterial infections but rather complex diseases of multifactorial complexity that interplay with the subgingival microbes, the host immune, and inflammatory responses. Despite dental plaque biofilm being considered the primary risk factor for periodontitis in the vast majority of patients that dentists encounter on a daily basis, there are other factors that can also contribute and/or accelerate pathologic progressive attachment loss. In this article, the authors aim to briefly review and discuss the present evidence regarding the association between periodontal diseases and systemic diseases and conditions.
Mathematics Applied to the Economy and Sustainable Development Goals: A Necessary Relationship of Dependence
Authors: Lafuente-Lechuga Matilde , Cifuentes-Faura Javier , Faura-Martínez Úrsula
Published: November 2020

Higher education must include training in sustainability to make all actors aware of the serious problems our planet is facing. Mathematics plays an important role in the achievement of the Sustainable Development Goals (SDG) and at the same time these allow working with real situations in the subject of mathematics, providing the student with active learning. Sustainability is used to make the student see the usefulness of mathematics while instilling values and attitudes towards it. A set of problems have been raised during the academic year that are solved with the developed mathematical techniques, and through a survey, the students’ perceptions about the usefulness of mathematics to reach the goals established in the SDG has been evaluated. The results show that, regardless of the student’s gender, the student’s assessment of the usefulness of this subject in solving real problems improved. It has been observed that this teaching methodology has helped to motivate students and even those who do not like this subject have improved their appreciation of it.
5G-Compliant Authentication Protocol for RFID
Authors: Munilla Jorge , Hassan Adel , Burmester Mike
Published: November 2020

The term “Internet of Things” was originally coined when radio frequency identification (RFID) technology was being developed to refer to applications where RFID tagged objects and sensors enabled computers to achieve effective situational awareness without human intervention. Currently, this term encompasses a myriad of medium/small devices connected to the Internet. On the other hand, 5G is a key enabling technology that will support next generation wireless communications. Moreover, 5G aims to realize the “Internet of Everything”. Surprisingly, despite the expected relationship between these two technologies, RFID tags have not been properly integrated into 4G and it is not clear if this will change in 5G. RFID is considered as a parallel technology where, at best, it has connection to the core network using back-end servers as gateways between the two technologies. With the aim of overcoming this problem, this paper proposes a 5G compliant RFID protocol that allows RFID tags to act as fully fledged 5G subscribers while taking into account the main characteristics of RFID systems. This proposal leverages the separation between USIM and mobile equipment within the user equipment to implement a 5G compliant protocol where tags accomplish the authentication part, as 5G subscribers, while readers assume the mobile equipment role, carrying out the 5G communication and most of the resource consuming tasks.
Sensing and Signal Processing in Smart Healthcare
Authors: Zhao Wenbing , Sampalli Srinivas
Published: November 2020

In the last decade, we have seen rapid development of electronic technologies that are transforming our daily lives [...]
Unsupervised Adversarial Defense through Tandem Deep Image Priors
Authors: Shi Yu , Fan Cien , Zou Lian , Sun Caixia , Liu Yifeng
Published: November 2020

Deep neural networks are vulnerable to the adversarial example synthesized by adding imperceptible perturbations to the original image but can fool the classifier to provide wrong prediction outputs. This paper proposes an image restoration approach which provides a strong defense mechanism to provide robustness against adversarial attacks. We show that the unsupervised image restoration framework, deep image prior, can effectively eliminate the influence of adversarial perturbations. The proposed method uses multiple deep image prior networks called tandem deep image priors to recover the original image from adversarial example. Tandem deep image priors contain two deep image prior networks. The first network captures the main information of images and the second network recovers original image based on the prior information provided by the first network. The proposed method reduces the number of iterations originally required by deep image prior network and does not require adjusting the classifier or pre-training. It can be combined with other defensive methods. Our experiments show that the proposed method surprisingly achieves higher classification accuracy on ImageNet against a wide variety of adversarial attacks than previous state-of-the-art defense methods.
A Low-Power Memory-in-Pixel Circuit for Liquid Crystal Displays Comprising Low-Temperature Poly-Silicon and Oxide Thin-Film Transistors
Authors: Kim Jongbin , Lee Woo-Rim , Chung Hoon-Ju , Lee Seung-Woo
Published: November 2020

In this paper, a new pixel structure using low-temperature polycrystalline silicon and oxide (LTPO) thin-film transistors (TFTs) for low-power liquid crystal displays (LCDs) is proposed. The extremely low off-state current of oxide semiconductor TFTs enables the proposed circuit to operate at a very low frame frequency of 1/60 Hz, so that the power consumption can be significantly reduced. In addition, the low-temperature polycrystalline silicon TFTs with high reliability directly drive pixels, which can achieve stable and flicker-free LCDs. The proposed circuit is fabricated using the LTPO TFT backplane and successfully verified by simulation and measurement results. The measurement results prove that the proposed circuit operates well without further programming for 60 s, and the power consumption in the panel (except backlight power) can be reduced to 0.02% of that of conventional LCD.
Selection of Suitable Reference Genes Based on Transcriptomic Data in Ginkgo biloba under Different Experimental Conditions
Authors: Zhou Tingting , Yang Xiaoming , Fu Fangfang , Wang Guibin , Cao Fuliang
Published: November 2020

Ginkgo biloba, a deciduous tree species in the Ginkgo family, has a long history of cultivation in China and is widely used in garden landscapes, medicine, food, and health products. However, few reports have focused on the systematic selection of optimal reference genes based on transcriptomic data in G. biloba. The purpose of our research was to select an internal reference gene suitable for different experimental conditions from thirteen candidate reference genes by the delta cycle threshold (ΔCt) method, geNorm, BestKeeper, NormFinder, and RefFinder programs. The reference genes were used for gene expression analyses of Ginkgo biloba. These results showed that elongation factor 1(EF1) and ubiquitin (UBI) were the best choices for samples of different ginkgo genotypes. The expression of UBI and HAS28 presented the most stable at different developmental stages of ginkgo, and EIF3I and RPII were considered as suitable reference genes in different tissues of ginkgo. For methyl jasmonate (MeJA) treatment, ACA and ACT were identified as the optimal reference genes. For cold stress treatment, RPII and EIF4E were chosen for the gene expression normalizations. HAS28 and GAPDH presented the most stable expression for the heat treatment. To validate the above results, a chalcone synthase gene (GbCHS) in ginkgo was amplified by quantitative real-time polymerase chain reaction (qRT-PCR). Our results provide different suitable reference genes for further gene expression studies in ginkgo.
Long-Term Changes of Softwood Floodplain Forests—Did the Disappearance of Wet Vegetation Accelerate the Invasion Process?
Authors: Mikulová Katarína , Jarolímek Ivan , Šibík Jozef , Bacigál Tomáš , Šibíková Mária
Published: November 2020

Objectives: We followed the long-term changes of softwood floodplain forests strongly altered by water regime changes and examine the behaviour of neophytes in this environment. Here we ask: (1) How did the composition of neophyte and native species change? (2) How did the presence of species that prefer wetter conditions change? (3) What traditionally distinguished type of softwood floodplain forests (a wetter one or a more mesophilous one) do neophytes prefer? (4) What environmental factors affect the native species richness and the occurrence and cover of neophytes? Materials and Methods: Historical and recent phytosociological relevés of the association Salicetum albae of the Slovak part of the inland delta of the Danube River were used (177 plots together). For each plot, the number and cover of neophytes and number of native species were measured, and the Shannon-Wiener diversity index, the stand structure (cover of tree, shrub and herb layer) and the mean of Ellenberg indicator values were calculated and compared among time periods. Temporal trends of the soil moisture characterized by indicator values calculated for each plot were determined using a Linear Model. The synoptic table of traditional vegetation types was done to show preferences of neophytes for particular softwood forest types. The effect of site conditions on native species richness and occurrence of neophytes was determined using the Generalized Linear Model. Results: The relative number and cover of neophyte species increased and the absolute number of native species decreased over time; the vegetation of the area has changed from variable hygrophilous and mesophilous to homogenised mesophilous; most non-native species prefer the mesophilous vegetation of the floodplain forests; the wetter parts of the floodplain more successfully resisted invasions. Conclusions: The vegetation of the researched area has considerably changed over time to become less diverse and less hygrophilous, and has more invasive species. To preserve floodplain forests, natural hydrological and connectivity patterns should be adequately protected.
Analyzing the Joint Effect of Forest Management and Wildfires on Living Biomass and Carbon Stocks in Spanish Forests
Authors: Adame Patricia , Cañellas Isabel , Moreno-Fernández Daniel , Packalen Tuula , Hernández Laura , Alberdi Iciar
Published: November 2020

Research Highlights: This is the first study that has considered forest management and wildfires in the balance of living biomass and carbon stored in Mediterranean forests. Background and Objectives: The Kyoto Protocol and Paris Agreement request countries to estimate and report carbon emissions and removals from the forest in a transparent and reliable way. The aim of this study is to forecast the carbon stored in the living biomass of Spanish forests for the period 2000–2050 under two forest management alternatives and three forest wildfires scenarios. Materials and Methods: To produce these estimates, we rely on data from the Spanish National Forest Inventory (SNFI) and we use the European Forestry Dynamics Model (EFDM). SNFI plots were classified according to five static (forest type, known land-use restrictions, ownership, stand structure and bioclimatic region) and two dynamic factors (quadratic mean diameter and total volume). The results were validated using data from the latest SNFI cycle (20-year simulation). Results: The increase in wildfire occurrence will lead to a decrease in biomass/carbon between 2000 and 2050 of up to 22.7% in the medium–low greenhouse gas emissions scenario (B2 scenario) and of up to 32.8% in the medium–high greenhouse gas emissions scenario (A2 scenario). Schoolbook allocation management could buffer up to 3% of wildfire carbon loss. The most stable forest type under both wildfire scenarios are Dehesas. As regards bioregions, the Macaronesian area is the most affected and the Alpine region, the least affected. Our validation test revealed a total volume underestimation of 2.2% in 20 years. Conclusions: Forest wildfire scenarios provide more realistic simulations in Mediterranean forests. The results show the potential benefit of forest management, with slightly better results in schoolbook forest management compared to business-as-usual forest management. The EFDM harmonized approach simulates the capacity of forests to store carbon under different scenarios at national scale in Spain, providing important information for optimal decision-making on forest-related policies.
Quantifications of Oleocolloid Matrices Made of Whey Protein and Oleogels
Authors: Park Clifford , Jimenez-Flores Rafael , Maleky Farnaz
Published: November 2020

Consumer demand for high protein content and plant-based fat has necessitated novel approaches to healthy food products. In response to this need, oleogels (OG) (structured liquid oils) emerged as a possible means of not only replacing saturated and trans fats but also delivering food protein. Nevertheless, an in-depth view of the structure of networks made of OG and protein is deficient. Hence, the objective of this study is developing oleocolloid (OC) (whey protein and rice bran wax OG) and hydro-oleocolloid (HOC) (OC + water) matrices with varying protein content (2.5–7.5%) to characterize their structural properties. Thermal analysis of the matrices via differential scanning calorimetry (DSC) documented the effects of hydrophobic interactions on the protein structure and its stability. Whey protein denaturation temperature increased from 74.9 °C to 102.8 °C in the presence of high oleic soybean oil. The effects of vegetable oil on WPI structure was also verified by FTIR spectroscopy. Data analysis revealed slight structural changes of the WPI secondary structure in the hydrophobic oil medium and the α-helix and β-sheet proportion in the emulsion medium was significantly altered. Similar analysis was performed in OC and HOC networks to quantify possible interactions between protein and rice bran wax. Results indicated that the protein was denatured during the thermal and mechanical conditions required for the oleogelation process, while it did not affect the systems’ solid fat content (SFC) and polymorphic patterns of the oleogels. However, DSC analysis showed different onset of melting for OC and HOC samples due to colloidal interactions between the protein and the lipid phase. The role of these chemistry was confirmed by microscopy analyses where OC and HOC matrices displayed notably different microstructural properties. The observed differences in the structural properties between OC and HOC matrices indicate the different colloidal interactions mediated by oleogelation process and the liquid medium type (oil vs. emulsion).
Formation and Outburst of the Toguz-Bulak Glacial Lake in the Northern Teskey Range, Tien Shan, Kyrgyzstan
Authors: Daiyrov Mirlan , Narama Chiyuki , Kääb Andreas , Tadono Takeo
Published: November 2020

In Kyrgyzstan, outburst flood disasters from glacial lakes are increasing. An example is the sudden drainage on 8 August 2019 of the Toguz-Bulak glacial lake in the Tosor river basin of the northern Tien Shan region. In this study, we used remote sensing and field surveys to examine the reasons for the outburst. We found that the lake area changed from 0.021 km² to 0.002 km2 due to the outburst, in which most of the initial 130,000 m3 of water discharged within four hours. In examining the longer-term behavior of this lake, we found that from 2010 through 2019, it appears in June and disappears in September every year. Its maximum area occurs in late July and early August. With the expansion of the lake basin between 2010 and 2019, the lake also increased greatly in size, particularly so in the three years before the outburst, linked to high summer temperatures and the resulting higher inflow of glacier meltwater, finally leading to the sudden drainage in 2019. Before this outburst, a 2-m high moraine dam retained the lake. Continuously inflowing meltwater and the related increasing pressure by the lake water mass eventually broke the moraine dam. Satellite radar interferometry revealed active displacement fringes in the lake basin and moraine dam due to the melting and subsidence of buried ice. An analysis using digital elevation models from 1964 and 2010 also confirms the surface lowering in the lake basin by up to 8.5 m and on the moraine dam by 2 m. Such lowering of the proglacial moraine complex destabilized the moraine dam.
The Struggle for Human Attention: Between the Abuse of Social Media and Digital Wellbeing
Authors: Giraldo-Luque Santiago , Aldana Afanador Pedro Nicolás , Fernández-Rovira Cristina
Published: November 2020

Human attention has become an object of study that defines both the design of interfaces and the production of emotions in a digital economy ecosystem. Guided by the control of users’ attention, the consumption figures for digital environments, mainly social media, show that addictive use is associated with multiple psychological, social, and physical development problems. The study presented develops a theoretical proposal regarding attention. In the first part, the research analyzes how attention has been studied and how it behaves using three disciplines: neurophysiology, neuropsychology, and economics. In the second part, considering this general framework, the study uses categories of the three disciplines to explain the functioning of social media, with special emphasis on their interactive, attractive, and addictive design. Finally, the article presents, as a practical example of the exposed theory, the main results of two case studies that describe social media consumption among young people. The research shows the relevance of the theoretical study of attention as a key element by which to understand the logics that dominate the interactive design of social media. It also uses a multidisciplinary perspective. The addictive behaviors identified in the two examples support the theoretical proposals and open research lines oriented to the measurement and understanding of the attention given to social media.
A Realist Evaluation of Theory about Triggers for Doctors Choosing a Generalist or Specialist Medical Career
Authors: O’Sullivan Belinda , McGrail Matthew , Gurney Tiana , Martin Priya
Published: November 2020

There is a lack of theory about what drives choice to be a generalist or specialist doctor, an important issue in many countries for increasing primary/preventative care. We did a realist evaluation to develop a theory to inform what works for whom, when and in what contexts, to yield doctors’ choice to be a generalist or specialist. We interviewed 32 Australian doctors (graduates of a large university medical school) who had decided on a generalist (GP/public health) or specialist (all other specialties) career. They reflected on their personal responses to experiences at different times to stimulate their choice. Theory was refined and confirmed by testing it with 17 additional doctors of various specialties/career stages and by referring to wider literature. Our final theory showed the decision involved multi-level contextual factors intersecting with eight triggers to produce either a specialist or generalist choice. Both clinical and place-based exposures, as well as attributes, skills, norms and status of different fields affected choice. This occurred relative to the interests and expectations of different doctors, including their values for professional, socio-economic and lifestyle rewards, often intersecting with issues like gender and life stage. Applying this theory, it is possible to tailor selection and ongoing exposures to yield more generalists.
Effect of a Simulated Match on Lower Limb Neuromuscular Performance in Youth Footballers—A Two Year Longitudinal Study
Authors: Lehnert Michal , De Ste Croix Mark , Zaatar Amr , Lipinska Patrycja , Stastny Petr
Published: November 2020

The aim of this study was to explore the effects of simulated soccer match play on neuromuscular performance in adolescent players longitudinally over a two-year period. Eleven players completed all measurements in both years of the study (1st year: age 16.0 ± 0.4 y; stature 178.8 ± 6.4 cm; mass 67.5 ± 7.8 kg; maturity-offset 2.24 ± 0.71 y). There was a significant reduction in hamstring strength after simulated match by the soccer-specific aerobic field test (SAFT90), with four out of eight parameters compromised in U16s (4.7–7.8% decrease) and six in the U17s (3.1–15.4%). In the U17s all of the concentric quadriceps strength parameters were decreased (3.7–8.6%) as well as the vastus lateralis and semitendinosus firing frequency (26.9–35.4%). In both ages leg stiffness decreased (9.2–10.2%) and reactive strength increased pre to post simulated match (U16 8.0%; U17 2.5%). A comparison of changes between age groups did not show any differences. This study demonstrates a decrease in neuromuscular performance post simulated match play in both ages but observed changes were not age dependent.
Relationships between Training Loads and Selected Blood Parameters in Professional Soccer Players during a 12-Day Sports Camp
Authors: Radzimiński Łukasz , Jastrzębski Zbigniew , López-Sánchez Guillermo F. , Szwarc Andrzej , Duda Henryk , Stuła Aleksander , Paszulewicz Jacek , Dragos Paul
Published: November 2020

The main purpose of this study is to assess the relations between training loads and selected blood parameters in professional soccer players during a preseason sports camp. Fifteen professional soccer players (age: 24.3 ± 5.25 year; height: 182.6 ± 6.75 cm; weight: 76.4 ± 6.72 kg) participated in the 12-day training camp. All the training sessions and friendly games were accurately analyzed with a GPS system. Blood samples were taken from the players and analyzed before the camp (PRE), in the middle (MID), and one day after the camp (POST). Mean total distance covered by the players during the camp was 85,205 ± 2685 m, high-intensity running 12,454 ± 1873 m, and sprinting 639 ± 219 m. The highest aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), and C-reactive protein (CRP) values were observed after six days of the camp. The application of intensive training during a 12-day sports camp can be associated with chronic muscle pain with high activity of some blood enzymes (CK, AST) and a high concentration of myoglobin (Mb). During training camps longer than 10 days, it would be necessary to apply, every second or third day, one day of rest, and the training load should not exceed two units every day.
Impact of Implementing a Dyslipidemia Management Guideline on Cholesterol Control for Secondary Prevention of Ischemic Heart Disease in Primary Care
Authors: Forcadell Drago Emma , Dalmau Llorca Maria Rosa , Aguilar Martín Carina , Ferreira-González Ignacio , Hernández Rojas Zojaina , Gonçalves Alessandra Queiroga , López-Pablo Carlos
Published: November 2020

Cardiovascular diseases (CVD) are the main cause of death worldwide. The control of CVD risk factors, such as dyslipidemia, reduces their mortality rate. Nonetheless, fewer than 50% of patients with ischemic heart disease (IHD) have good cholesterol control. Our objective is to assess whether the level of participation of general practitioners (GPs) in activities to implement a dyslipidemia management guideline, and the characteristics of the patient and physician are associated with cholesterol control in IHD patients. We undertook a quasi-experimental, uncontrolled, before-and-after study of 1151 patients. The intervention was carried out during 2010 and 2011, and consisted of a face-to-face training and online course phase (Phase 1), and another of face-to-face feedback (Phase 2). The main outcome variable was the low-density lipoprotein cholesterol (LDL-C) control, whereby values of <100 mg/dL (2.6 mmol/L) were set as a good level of control, according to the recommendations of the guidelines in force in 2009. After Phase 1, 6.7% more patients demonstrated good cholesterol control. With respect to patient characteristics, being female and being older were found to be risk factors of poor control. Being diabetic and having suffered a stroke were protective factors. Of the GPs’ characteristics, being tutor in a teaching center for GP residents and having completed the online course were found to be protective factors. We concluded that cholesterol control in IHD patients was influenced by the type of training activity undertook by physicians during the implementation of the GPC, and patient and physician characteristics. We highlight that if we apply the recent targets of the European guideline, which establish a lower level of LDL-C control, the percentage of good control could be worse than the observed in this study.
Impact of the Relative Age Effect on Competition Performance in Basketball: A Qualitative Systematic Review
Authors: de la Rubia Riaza Alfonso , Lorenzo Calvo Jorge , Mon-López Daniel , Lorenzo Alberto
Published: November 2020

Performance in basketball is multifactorial. One of the modifying factors is the “Relative Age Effect—RAE”. However, its impact depends on the sample characteristics and sport context. The purpose of this study was to examine the influence of the RAE on basketball competition performance by analysing peer-reviewed articles published until July 2020. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses systematic search guidelines, nine studies were identified in four databases: Sport Discus, PubMed, Web of Science, and Scopus. Moreover, a study quality analysis using “Strengthening the Reporting of Observational Studies in Epidemiology” guidelines was carried out. The results confirmed an impact of the RAE on competition performance in basketball (56% measurements) and a higher influence of the RAE on short-term collective performance (54% measurements). Statistical parameters were affected, especially in men and U14-U18 categories. No impact of the RAE reversal and no influence of the RAE on long-term collective performance were found. There was a higher impact of the RAE in men (71%), the U14-U18 categories (44%), and at the national level (40%) was identified. The RAE has a variable influence on basketball performance according to developmental constraints. Nevertheless, the findings should be considered based on the sport context due to the heterogeneity and variability of the identified results.
How the COVID-19 Pandemic Changes the Subjective Perception of Meaning Related to Different Areas of Life in Austrian Psychotherapists and Patients
Authors: Humer Elke , Schimböck Wolfgang , Kisler Ida-Maria , Schadenhofer Petra , Pieh Christoph , Probst Thomas
Published: November 2020

We assessed psychotherapists’ and patients’ ratings of their subjective perception of meaning related to different areas of life before the COVID-19 pandemic as compared to the time during the COVID-19 pandemic. In a quantitative cross-sectional study, Austrian psychotherapists (N = 222) were recruited by e-mail, who in turn recruited their patients (N = 139). Therapists and patients were asked to rate the meaning of different areas of life before as well as during the COVID-19 crisis. The psychotherapists showed an overall higher rating of the importance of areas of life compared to their patients (p < 0.001). The rating of the importance of the domains of living was differently affected by the COVID-19 situation (p < 0.001). While the meaning of physical and mental health during COVID-19 was rated higher than before, the opposite was observed for work (p < 0.001). No differences were found for relationships and friends, as well as for hobbies. As no interactions between perspective (therapists vs. patients), area of life, and time point (before vs. during COVID-19) were observed, it can be concluded that the COVID-19 situation changed the subjective attribution of meaning concerning different aspects of life similarly in therapists as well as patients. While mental and physical health gained subjective importance, the opposite was observed for work.