bluuebunny/crossref_metadata_2025 huggingface dataset:
Rate of Cardiotoxicity in Childhood Acute Lymphoblastic Leukemia Treated with Daunorubicin Using Echocardiography and Troponin I
Authors: Sultana Tania , Chowdhury Tapas , Islam Farzana , Ara Umme Nusrat , Kibria Chowdhury Shamsul Hoque , Jamal Chowdhury Yakub , Rahman A T M Atikur , Karim Anwarul
Published: November 2023

Background and Aim: Acute lymphoblastic leukemia (ALL) is the commonest malignancy in childhood. Childhood ALL Survivors have a lifelong increased risk for cardiovascular morbidity and mortality compared to the general population, mainly caused by chemotherapy with daunorubicin. The aim of the study is to detect the rate of daunorubicin induced cardiotoxicity in children with acute lymphoblastic leukemia during induction phase chemotherapy.
Meterials & Methods : This prospective observational study was conducted in the department of Pediatric Hematology and Oncology, Bangabandhu Sheikh Mujib Medical University (BSMMU) on 40 newly diagnosed patients of ALL aged between 1 to17.9 years who got daunorubicin during induction. Complete blood count and echocardiography were done and troponin I was measured in all patients before and after completion of induction period.
Result: Of the 40 patients, 8 patients (20%) had developed cardiotoxicity evidenced by reduction of left ventricular ejection fraction (LVEF) in echocardiography. Baseline LVEF was 68.80±5.98% which was then reduced to 65.32±7.07% after induction phase of chemotherapy (p=0.023). No significant alteration of troponin I was seen (P= 0.581) between baseline and after completion of induction. Total WBC count and hemoglobin had a significant difference (P<0.05) between baseline and after induction period. Male patients had a greater risk of developing cardiotoxicity than females but statistically was not significant (P=0.643). There was no significant association between age of the patients and cardiotoxicity (P=0.112).Cardiotoxicity was seen higher in patient with initial high WBC count (p=0.039). Echocardiography also reveled increased tendency of mitral regurgitation and left ventricular hypertrophy after induction phase chemotherapy.
Conclusion: This study showed the rate of cardiotoxicity was 20% in ALL patients treated with daunorubicin. It also found that LVEF was decreased during therapy.Echocardiography can be used to detect early cardiotoxicity induced by daunorubicin.
University Heart Journal 2023; 19(1): 20-25
Tinjauan Kontribusi Fosil dalam Penetapan Warisan Geologi
Authors: Kresna Dewi  , Erik Setiyabudi  , Riecca Oktavitania  , Hanang Samodra
Published: November 2023

Dokumen usulan penetapan situs warisan geologi dari Pemerintah Daerah Kota, Kabupaten dan Provinsi di Indonesia makin meningkat sejak beberapa tahun terakhir ini.Situs Warisan Geologi tersebut ditetapkan berdasarkan keragaman komponen geologi (batuan, mineral, struktur, bentang alam dan fosil) yang memiliki nilai lebih sebagai suatu warisan terkait rekaman atas suatu peristiwa geologi. Hingga pertengahan tahun 2023 telah terbit 11 (sebelas) Keputusan Menteri Energi dan Sumber Daya Mineral yang menetapkan 236 situs warisan geologi kepada 12 (duabelas) Kabupaten dan 2 (dua) Provinsi di Indonesia. Komponen fosil berkontribusi pada 28 situs warisan geologi dalam memberi sumbangsih bernilai ilmiah lokal hingga terkemuka dengan ditemukannya beragam fosil di Kabupaten Merangin, Tulungagung dan Gorontalo. Ketersediaan data fosil mendukung data suatu daerah yang mengajukan penetapan warisan geologi. Tujuan dari tulisan ini adalah menyediakan data fosil di beberapa daerah yang belum memasukkan komponen fosil. Selain itu juga menyajikan contoh proses penetapan warisan paleontologi sebagai bagian dari konservasi geologi di masa depan. Data paleontologi mempunyai nilai-nilai ilmiah, edukasi dan pariwisata berbasis geologi yang perlu dilindungi (geokonservasi). Hal ini dalam upaya memberi manfaat peningkatan perekonomian baik langsung maupun tidak langsung kepada masyarakat.
Katakunci: Warisan Geologi, fosil, nilai ilmiah, edukasi, konservasi
APLIKASI PRESENSI ONLINE MENGGUNAKAN VALIDASI JARAK LOKASI PENGGUNA BERBASIS ANDROID
Authors: Permana Bayu Adytia
Published: November 2023

Presensi online adalah sebuah sistem yang digunakan untuk pencatatan kehadiran di tempat kerja secara online sebagaimana dibutuhkan untuk memepermudah pencatatan presensi, dimana data kehadiran ini dapat disimpan dan digunakan untuk kepentingan dalam memudahkan pembuatan laporan kehadiran kerja. Presensi online juga efisien penggunaannya karena dapat digunakan dimana saja. Presensi online tersebut akan menggunakan perangkat smarthpone yang menggunakan sistem operasi android di karenakan perangkat android yang mengalami pertumbuhan yang sangat pesat dan sangat banyak digunakan oleh masyarakat dan juga sistem android menyediakan platform terbuka bagi para pengembang untuk menciptakan aplikasi mereka di berbagai macam perangkat bergerak.
SISTEM INFORMASI PKK BERBASIS WEBSITE MENGGUNAKAN FRAMEWORK CODEIGNITER (STUDI KASUS: KAMPUNG PURWOEJO)
Authors: Setiawansyah Setiawansyah , Lestari Dinda Titian , Megawaty Dyah Ayu
Published: November 2023

Penyelenggaraan PKK merupakan salah satu tugas pemerintah desa atau kelurahan. Setiap desa atau kelurahanÂ memiliki kelompok PKK yang mendukung pemerintah desa atau kelurahan dalam pengelolaan kependudukanÂ dan pembangunan wilayah. Salah satunya desa/kampungpurworejo kecamatan kota gajah kabupaten lampungÂ tengah. Kampung purworejo adalah desa yang memiliki peranan penting dalam memberdayakan wanita untukÂ berpartisipasi dalam pembangunan desa dengan organisasi PKK yang dicanangkan pemerintah.OrganisasiÂ PKK memiliki sebuahstruktur organisasi.PKK juga mempunyai sebuah kelompok kerja (POKJA) dan setiapÂ POKJA memegang peranan kegiatan yang berbeda.Selama kegiatan PKK saat ini rekap hasil kegiatanÂ dilakukan secara manual di buku sekretaris. Dengan berkembangnyasistem informasi, proses pencatatan danÂ pendokumentasian kegiatan PKK harus terekap secara rapi dan terorganisir.Untuk memberikan solusi makaÂ perlunya dikembangkanSistemInformasiberbasis website dibangun dengan menggunakan metode penelitianÂ waterfalldengan pendekatan menggunakan UML sepertiusecase, activitydiagram,class diagramdll. HasilÂ dari penelitian ini adalah sistem informasiPKKberbasis websiteyang mencakup keseluruhan kegiatan PKK,Â termasuk diantaranya pendataan anggota,penyajian informasidandokumentasi kegiatan setiapPOKJA yangÂ akan membantu para petugas dalam menjalankan tugasnya dengan baik.
PENGEMBANGAN GAME PETUALANGAN EDUKASI PENGENALAN SATWA DILINDUNGI DI INDONESIA MENGGUNAKAN CONSTRUCT 2
Authors: Janata Ridwan , Priandika Adhie Thyo , Gunawan Rahmat Dedi
Published: November 2023

Pada era kemajuan teknologi saat ini perkembangan game sudah sangat pesat, terutama pada game yang dibuat untuk perangkat berbasis teknologi mobile. Game mobile adalah game yang dimainkan pada handphone, smartphone, PDA, dan tablet. Namun dibalik pesatnya perkembangan teknologi di Indonesia, populasi satwa â€“ satwa langka endemik di Indonesia masih terus mengalami penurunan dan mulai mengalami kepunahan, hal ini terjadi karena maraknya perburuan liar dan perusakan habitat mereka, Dibutuhkan media yang dapat memperkenalkan dan memberi informasi mengenai satwa langka yang dilindungi. Salah satu media yang paling menyenangkan dan dapat dengan mudah diterima dengan baik adalah melalui suatu game. Oleh karena itu keluarlah ide untuk membuat game edukasi yang dapat memperkenalkan dan menampung informasi mengenai satwa â€“ satwa langka Indonesia. Dalam penelitian ini proses pengembangan menggunakan metode GDLC (Game Development Life Cycle), Penulis membuat game berbasis mobile android dan menggunakan Construct 2 untuk mengembangkan game, dengan berdasarkan pengujian menggunakan aspek Usability ISO 9126 mendapat nilai presentase rata â€“ rata 89%.
UPAYA MENINGKATKAN CITRA DIRI MELALUI GAME EDUKASI PENGEMBANG KEPRIBADIAN BERBASIS MOBILE
Authors: Arifah Septi Nurul , Fernando Yusra , Rusliyawati Rusliyawati
Published: November 2023

Pengembangan Kepribadian merupakan pembelajaran yang dapat mengendalikan diri sendiri, kecerdasan emosional, lingkungan tempat berkatifitas atau kerja, kondisi dan disiplin diri, kemampuan mengelola diri atau emosi, kemampuan menghadapi situasi yang dihadapi. Minat serta ketertarikan mahasiswa dalam mempelajari pengembangan kepribadian masih sangat rendah hal ini mengakibatkan pandangan mahasiswa terhadap materi tersebut sebagai hal yang menyulitkan diri mereka, tidak banyak juga mahasiswa yang hanya sekedar mempelajarinya tetapi tidak menerapkan dikehidupan sehari-hari. Hal tersebut yang menjadi permasalahan bagi pengajar Pengembangan Kepribadian dalam menarik perhatian mahasiswa supaya lebih tertarik dalam belajar Pengembangan Kepribadian. Untuk menyelesaikan permasalahan tersebut peneliti membangun aplikasi media pembelajaran yang menarik berupa game edukasi yang dapat meningkatkan citra diri serta minat belajar mahasiswa dalam pembelajaran pengembangan kepribadian berbasis mobile. Aplikasi game edukasi pada pembelajaran pengembangan kepribadian layak digunakan hal ini dapat dilihat dari hasil pengujian yang dilakukan dengan black-box testing yang mendapatkan nilai 100% untuk dosen ahli IT dan 100% untuk Mahasiswa. Tidak hanya itu game edukasi ini juga dapat meningkatkan citra diri serta minat belajar mahasiswa dalam pembelajaran.
RANCANG BANGUN APLIKASI PENJUALAN BERBASIS ANDROID (Studi Kasus: PADA TOKO MURAH JAYA ALUMUNIUM)
Authors: Aditya Feri , Putra Ade Dwi , Surahman Ade
Published: November 2023

Toko Murah Jaya Alumunium adalah Toko yang bergerak di bidang mabel atau funiture Alumunium. Perkembangan produksi alumunium saat ini sangatlah diminati oleh masyarakat dari mulai lemari baju, lemari hias, kichen set, lemari belajar dan lain-lain. akan tetapi perkembangan penjualan alumunium masih terkendala beberapa masalah pada penjualan dan pemasaran. ketika stok habis pembeli bisa datang atau memesan melalui Salesman dengan memesan produk yang di inginkan. Berdasarkan permasalahan di Toko Murah jaya alumunium saya memberikan solusi dengan cara membuatkan satu aplikasi yang nantinya bisa membantu proses penujualan dan pemasaran. Dilihat dari berkembangnya teknologi informasi saat ini perlunya inovasi agar proses penjualan dan pemasaran bisa tersebar luas. Dengan adanya aplikasi ini nantiya akan memudahan pembeli untuk melihat produk dan stok yang ada. Maka perlunya suatu aplikasi android yang membantu proses penjualan. Hasil dari proses merancang dan membangun aplikasi penjualan dengan android pada toko Murah Jaya Alumuniumm mendapatkan presentase 92,52% dengan skala â€œSangat Baikâ€ dan telah meninjau dari aspek- aspek Functional Suitability, efficiency dan usability. Dengan menggunakan metode Extrame Progrraming yang terdiri dari empat tahapan yaitu planning, design, coding, dan test.
Game Edukasi Pengenalan Dampak Buruk Merokok Bagi Kesehatan Berbasis Android
Authors: Ashari Nugraha , Darwis Dedi , Kisworo Kisworo
Published: November 2023

Tujuan dari penelitian membangun aplikasi game no smoke berbasis android sehingga membantu anak-anak agar lebih memahami dan mengenal bahaya merokok dan mengenalkan pengetahuan tentang bahaya merokok bagi anak-anak sehingga dapat menambah wawasan mereka. dengan melalui aplikasi game no smoke berbasis android. Dalam pengembangan sistem menggunakan metode extreme programming. Tahap perancangan menggunakan pemodelan UML yaitu: use case diagam, sequence diagram dan class diagram dan pembangunan sistem menggunakan bahasa pemograman yang digunakan dalam pembangunan sistem adalah C# (C sharp) dengan tools Unity 3D sebagai sebagai game engine. Berdasarkan hasil analisis karakteristik aplikasi game no smoke menggunakan model pengujian ISO 9126 yaitu karakteristik functionality diperoleh nilai 80% atau baik, karakteristik usability diperoleh nilai 80% atau baik. Berdasarkan kelayakan seluruh karakteristik kualitas maka didapat nilai rata-rata sebesar 80% atau baik. Dapat disimpulkan bahwa game no smoke sudah memenuhi karakteristik functionality dan karakteristik functionality dan layak untuk digunakan anak-anak sebagai sarana untuk lebih memahami dan mengenal bahaya merokok.
Sistem Pendukung Keputusan Penentuan Siswa Berprestasi Menggunakan Metode Weight Product (WP)
Authors: Mardian Demi , Neneng Neneng , Puspaningrum Ajeng Savitri , Hasibuan Alfiansyah , Tinambunan Medi Hermanto
Published: November 2023

Penilaian dilakukan oleh sistem dengan tetap mengikuti kriteria-kriteria yang ditentukan. Metode yang di pakai dalam pengambilan keputusan pemilihan siswa berprestasi salah satunya yang dapat digunakan adalah dengan menggunakan weight product (WP). Metode WP merupakan solusi pemilihan Siswa Berprestasi karena merupakan algoritma yang mampu mengkomodasi banyaknya kriteria dan menghasilkan solusi. Algoritma ini menghasilkan alternatif dengan membrikan bobot pada masing-masing kriterianya. Kriteria yang akan di gunakan untuk penelitian ini adalah nilai rata-rata raport, nilai extrakulikuler, nilai kedisiplinan, nilai kehadiran, dan nilai non akademik kegiatan. Implementasi sistem denagn melakukan penerapan secara langsung sistem informasi kepada guru yang mangajar dan bagian operator sekolah, serta melakukan pengujian sistem kepada 2 Dosen dan 8 guru atau operator sekolah, serta denagn metode pengujiannya menggunakan ISO 25010, pengujian dilakukan yaitu pada aspek fungsionality, dan oprability, dengan masing-masing nilai didapat untuk fungsioallity 90,91%, untuk oprability 78% dengan hasil pengujian tersebut maka sistem informasi dinyatakan layak untuk mengatasi penentuan siswa berprestasi.
Penerapan Algoritma Apriori untuk Menentukan Tata Letak Barang (Studi Kasus: Swalayan S&M Mart)
Authors: Martiwansyah Taufiq , Alita Debby , Isnain Auliya Rahman , Nuroji Nuroji
Published: November 2023

Saat ini teknologi informasi berkembang begitu cepat sehingga kebutuhan terhadap informasi semakin meningkat, Begitupun persaingan dunia bisnis untuk senantiasa mengembangkan bisnis mereka dan juga agar selalu bertahan dalam persaingan,Untuk mencapai hal itu dilakukan dengan meningkatkan kualitas produk, penambahan jenis produk, pengurangan biaya operasional dan dilakukan analisis data di sebuah toko, Pengaturan tata letak (layout) merupakan suatu keputusan penting untuk menentukan efisiensi sebuah Manajemen operasional secara jangka panjang. Dengan menggunakan data transaksi penjualan pada tahun 2020-2021 sebanyak 2.024 transaksi dan minimal support 30% dan confidence 80% yang sudah ditetapkan didapatkan hasil 15 rules dengan 2 kombinasi item menghasilkan produk yang sering dibeli bersamaan dengan confidence 0,805 yang terendah sampai dengan confidence 0,836 yang tertinggi, Berdasarkan pembahasan dengan penerapan Algoritma Apriori dalam rapidminer yang sering dibeli bersamaan sehingga disarankan untuk mengoptimasi apakah di pakai promo atau tata letaknya diatur kembali, sehingga produk ini bisa di letakan secara bersamaan atau berdekatan.
Media Pembelajaran Aksara Jepang Berbasis Android untuk Siswa SMA Kelas X
Authors: Ramadhan Raden Gamma , Surahman Ade
Published: November 2023

Bahasa Jepang merupakan salah satu bahasa asing yang paling populer saat ini, dan banyak orang yang ingin belajar bahasa Jepang, namun nyatanya masih banyak orang yang merasa bahasa Jepang sulit untuk dipelajari, terutama kanji dasar bahasa Jepang, karena bentuknya yang berbeda-beda. Berdasarkan permasalahan yang telah diuraikan, dalam penelitian ini penulis mengajukan permasalahan pembuatan media pembelajaran alternatif pembelajaran aksara hiragana dan katakana bahasa Jepang agar dapat menarik minat belajar dan memberikan pengalaman belajar yang baru bagi siswa sekolah menengah nasional 3 Bandar Lampung. Penelitian ini dilatarbelakangi oleh pengembangan metode pembelajaran yang dapat digunakan sebagai alat bantu untuk membantu proses kegiatan mengajar. Dengan dibuatnya aplikasi game edukasi bahasa Jepang ini diharapkan dapat membantu peneliti membuat game edukasi sekaligus proses pembelajaran di lokasi studi kasus penulis. Pada dasarnya konsep game edukasi adalah belajar sambil bermain agar pengguna tidak bosan saat mempelajari karakter hiragana dan katakana bahasa Jepang. Pembuatan game edukasi ini menggunakan pendekatan siklus hidup pengembangan game, dimana penulis melalui beberapa tahapan. Basis pengetahuan pembuatan game edukasi ini adalah melalui studi literatur pada jurnal-jurnal yang ada. Hasil dari penelitian ini adalah sebuah game edukasi untuk pembelajaran bahasa Jepang pada materi aksara Hiragana dan Katakana.
Inventário florístico do Brejo do Criador, uma área úmida no vale dos tabuleiros costeiros do Espírito Santo
Authors: Valadares Rodrigo Theófilo , Calazans Luana S. B. , Pereira Isabela de Lima , Gomes José Manoel Lúcio , Dutra Valquíria Ferreira
Published: November 2023

A vegetação aquática e seus atributos, como a capacidade de reter poluentes, são importantes indicadores da qualidade dos ecossistemas aquáticos. Entretanto, a flora destes ecossistemas em fundo de vales nos tabuleiros costeiros do Espírito Santo nunca foi avaliada, mesmo com as profundas alterações no uso do solo advindos de grandes empreendimentos portuários nos anos recentes. Neste trabalho, realizamos um levantamento florístico e demonstramos como a vegetação aquática nativa se distribui ao longo da zonação provocada por pulsos de inundação no Brejo do Criador, no extremo sul do estado. A área caracteriza-se pela ocorrência de 48 espécies, 36 gêneros e 16 famílias e vegetação predominantemente herbácea, com arbustos emergentes e espécies tolerantes à inundação, mas sua estrutura é bastante afetada pelo pisoteio e herbivoria do gado. Apesar disso, os pulsos de inundação garantem a permanência de espécies típicas de ambientes aquáticos. Nossos resultados permitem reduzir o negligenciamento ambiental dessas áreas e trazem valores de referência futuramente importantes para áreas semelhantes, principalmente em função da rapidez de alteração do uso do solo e crescimento populacional na região.
Development and Preclinical Trials of Castomized Porous Titanium Implants with Bioactive Coatings in Model Systems
Authors: Karalkin P. A. , Kudrin K. G. , Svyatoslavov D. S. , Usatov D. A. , Reshetov I. V.
Published: November 2023

This paper presents a technology for creating customized porous titanium implants with bioactive coatings, manufactured using additive technologies. The stages of creating an implant include obtaining primary data from the bone defect area using computed tomography; 3D modeling of the defect area and the corresponding implant; production of a customized implant from titanium alloys using selective laser fusion technology; application of bioactive coatings. The as-created customized implant can have several functional structures. Samples of titanium implants with bioactive coatings were subjected to extensive testing. Mathematical modeling and experiments were used to verify the correspondence of the mechanical properties of the developed structures to natural bone tissue. In vitro tests of the studied samples showed the absence of acute toxicity along with high levels of biocompatibility. In vivo tests of the studied samples on Soviet chinchilla rabbits and Anubis baboon monkeys approved by local ethical committees showed their adequate biomechanical and high osteoinductive properties. The successful results of preclinical studies, as well as toxicological and technical tests in certified laboratories, made it possible to create a registration dossier for state registration of customized porous titanium implants with bioactive coatings, manufactured using additive technologies.
Pharmacokinetics of a New Dimethylamineethanol Derivative Administered Orally to Laboratory Animals
Authors: Kim A. E. , Shustov E. B.
Published: November 2023

A new derivative of dimethylaminoethanol, butanedioic and trans-butenedioic acids (laboratory code ADK-17) was synthesized at the Organic Chemistry Department of the Saint Petersburg State Chemical and Pharmaceutical University (SPSPU). This promising nootropic and anti-asthmatic agent is planned for use in an oral dosage form. The aim was to calculate the pharmacokinetic parameters of the synthesized compound and to determine its possible biotransformation pathways. Rabbits of the Soviet Chinchilla breed were used as a test system in the preclinical study of the compound pharmacokinetics. In accordance with the designed schedule, blood was taken from the marginal vein of the ear, and urine samples were taken. Quantitative determination in biological media was carried out using an Ultimate 3000 liquid chromatograph with a Q-Exactive mass-selective detector with electrospray ionization in accordance with OFS 1.2.1.100015 (Chromatography) of the State Pharmacopoeia by ultra-performance liquid chromatography–mass spectrometry (UPLC-MS). To study the pharmacokinetics of the ADK-17 preparation, a measurement procedure was developed with a detection limit in biological media ranging from 10-6 to 10-3 mg/ml. The determined harmacokinetic parameters show that the absorption of ADK-17 into the systemic circulation from the gastrointestinal tract occurs at an average rate, with its maximum concentration in blood plasma during the oral route of administration being observed between 60–90 min. The elimination half-life from the systemic circulation comprises 4.3 h. Urinary excretion in the first 4 h proceeds at a high rate; however, the compound is detected in the urine within 3 days and in the blood within 2 days from the moment of administration.
Response of Mouse Ovaries to Stimulation witch ihe FSH-SU-PER Drug
Authors: Zhukova O. B. , Koloskova E. M.
Published: November 2023

Hormonal preparation of female egg donors is one of the first stages in obtaining genetically modified animals by microinjected zygote transplantation. A large number of embryos synchronized in development can be obtained by a superovulation procedure based on treating female animals with gonadotropins. As a rule, drugs from different manufacturers differ markedly in terms of their efficacy. In the context of sanctions imposed on the Russian Federation, attention should be paid to domestic hormonal drugs, thus substantiating their objective assessment. It was shown that different batches of the FSH-SUPER drug, used mainly for hormonal treatment of farm animals, differed in stimulating effect on superovulation in female mice. At the same time, the efficacy of this drug surpassed that of Follimag (Mosagrogen, Russia).
Obtaining Overstable Methionine Aminopeptidase for the Removal of Methionine From Recombinant Proteins
Authors: Lapteva Yu. S. , Bykov V. V. , Trunilina M. V. , Boldaevsky I. S. , Kudryashov T. A. , Vologzhannikova А. A. , Sokolov A. S.
Published: November 2023

Cleavage of the N-terminal initiating methionine (Met1) is a critical coand post-translational modification affecting 50–70% of cellular proteins. During the production of recombinant proteins in the heterologous system of E. coli expression, Met1 cleavage often fails to occur, which leads to heterogeneity of the preparations obtained, changes in their activity and stability. This problem can be solved by treating recombinant proteins in vitro with a specific enzyme, methionine aminopeptidase (MAP). Currently available MAPs exhibit limited specificities and reaction conditions. We cloned a MAP from a hyperthermophilic bacterium, developed a method for enzyme purification, and studied a number of physicochemical properties. The new MAP enzyme is resistant to elevated temperatures. The MAP maintains a stable native state in a pH range from 3 to 11 units. The novel MAP enzyme can be used to remove N-terminal Met1 from recombinant proteins in vitro over a wide pH range and at elevated temperatures.
HLDF-6-H Peptide Exhibits an Anti-inflammatory Effect in the Development of Depression in an MPTP Model of Parkinson's Disease
Authors: Zozulya S. A. , Sokolov O. Yu. , Kost N. V. , Baimeeva N. V. , Dadayan A. K. , Zolotarev Yu. A.
Published: November 2023

In this work, we investigate the anti-inflammatory effect of the HLDF-6-H peptide (Thr-Gly-Glu-HseHis-Arg) in a model of the presymptomatic stage of Parkinson’s disease (PD). This peptide is a bioactive fragment of the HLDF leukocyte differentiation factor. The presymptomatic stage of PD was modelled by introducing moderate doses of MPTP toxin to C57Bl/6 mice. HLDF-6-H was administered intranasally at a dose of 300 μg/kg daily for three weeks. The severity of depression was determined by the time of immobilization in a Porsolt test. The serum levels of 10 steroids were determined using MS analysis. The activity of inflammatory markers leukocyte elastase (LE) and α1-proteinase inhibitor (α1-PI) was determined using kinetic methods. The development of an inflammatory process under the influence of MPTP is evidenced by an increase in the activity of α1-PI; the anti-inflammatory effect of the peptide is manifested in a decreased activity of LE and α1-PI. At the same time, the immobilization time of mice in a Porsolt test positively correlated with α1-PI and LE, which may indicate the role of inflammation in the development of depression. A positive correlation between estradiol and LE was found in the general sample of mice. In the group of mice treated with the peptide, α1-PI correlated positively with corticosterone and negatively with estradiol and androstenedione. It can be assumed that the anti-inflammatory effect of the peptide is associated with the regulation of corticosteroids and sex hormones. Hence, the applied experimental model confirmed the development of a depressive-like syndrome and neuroendocrine disorders in the presymptomatic stage of PD. The long-term use of HLDF-6-H has antidepressant and anti-inflammatory effects.
Influence of Dioscorea Extracts on Physical Load Tolerance in the Setting of Hypoxic and Temperature Factors
Authors: Ivkina A. S. , Ivkin D. Yu. , Shustov E. B. , Povydysh M. N.
Published: November 2023

A comparative evaluation of the effectiveness of dry alcoholic extracts of Dioscorea from pharmacopoeial raw materials and cell cultures was carried out on a model of combined exposure to hypoxic and temperature factors and swimming of laboratory animals (mice) with a load of 5% of body weight. Dioscorea preparations had a pronounced thermoprotective effect and increased physical performance; however, the aftereffect was confirmed only for the cell culture extract.
Unexplored agronomic, socioeconomic and policy domains for sustainable cotton production on small landholdings: a systematic review
Authors: Tlatlaa Jacob Shauri , Tryphone George Muhamba , Nassary Eliakira Kisetu
Published: November 2023

Cotton productivity continues to be disputed, despite rapid advancements and widespread technologies. These uncertainties remain to be critically addressed in a broad spectrum focusing on domains at the global level. Therefore, this systematic review provides an overview of the existing advancements in knowledge, skills, and technologies for sustainable cotton production on small landholdings. Specifically, the areas of the cotton chain examined are threefold: - (1) Explore disguised agronomic practices to be endowed for sustainable cotton production on small landholdings; (2) Explore socioeconomic settings based on their disparities in contributing to sustainable cotton production on small landholdings; and (3) Explore existing and feasible institutional policies to be enforced for sustainable cotton production on small landholdings. This review shows that worldwide cotton production involves conventional and organic systems, at the expense of the traditional system. Heavy uses of nitrogenous fertilizers and pesticides are the common practices in conventional systems, with some adoptions of precision agriculture practices, and genetically modified varieties. Rotation and intercropping with early-maturing food crops are also identified viable options to improve farmers’ attitudes toward adopting cotton-producing technologies. In socioeconomics, farmers’ livelihoods are improved by income generation from sales of cotton and labour in the cotton industry. Gender equity in the cotton industry prioritizes females over males, as females display a group with a higher level of technology adoption. Generally, clear institutional policies governing the cotton industry are globally paucity. Furthermore, efforts to sensitize sustainable cotton production are still highly questionable and challenged by the superseding climate changes.
Echocardiographic functional determinants of survival in heart failure with abnormal ejection fraction
Authors: Ciampi Quirino , Cortigiani Lauro , Gaibazzi Nicola , Rigo Fausto , Zagatina Angela , Wierzbowska-Drabik Karina , Kasprzak Jaroslaw D. , Djordjevic-Dikic Ana , Haberka Maciej , Barbieri Andrea , Bartolacelli Ylenia , Pepi Mauro , Carerj Scipione , Villari Bruno , Pellikka Patricia A. , Picano Eugenio
Published: November 2023

Background and Aims

Patients with heart failure (HF) with reduced left ventricular (LV) ejection fraction (EF) have a heterogeneous prognosis, and assessment of coronary physiology with coronary flow velocity (CFV) and coronary flow velocity reserve (CFVR) may complement established predictors based on wall motion and EF.

Methods and results

In a prospective multicenter study design, we enrolled 1,408 HF patients (age 66 ± 12 years, 1,035 men), with EF <50%, 743 (53%) with coronary artery disease, and 665 (47%) with normal coronary arteries. Recruitment (years 2004–2022) involved 8 accredited laboratories, with inter-observer variability <10% for CFV measurement. Baseline CFV (abnormal value >31 cm/s) was obtained by pulsed-wave Doppler in mid-distal LAD. CFVR (abnormal value ≤2.0) was assessed with exercise (n = 99), dobutamine (n = 100), and vasodilator stress (dipyridamole in 1,149, adenosine in 60). Inducible myocardial ischemia was identified with wall motion score index (WMSI) stress > rest (cut-off Δ ≥ 0.12). LV contractile reserve (CR) was identified with WMSI stress < rest (cutoff Δ ≥ 0.25). Test response ranged from score 0 (EF > 30%, CFV ≥ 32 cm/s, CFVR > 2.0, LVCR present, ischemia absent) to score 5 (all steps abnormal). All-cause death was the only endpoint. Results. During a median follow-up of 990 days, 253 patients died. Independent predictors of death were EF (HR: 0.956, 95% CI: 0.943–0.968, p < 0.0001), CFV (HR: 2.407, 95% CI: 1.871–3.096, p < 0.001), CFVR (HR: 3.908, 95% CI: 2.903–5.260, p < 0.001), stress-induced ischemia (HR: 2.223, 95% CI: 1.642–3.009, p < 0.001), and LVCR (HR: 0.524, 95% CI: 0.324–.647, p = 0.008). The annual mortality rate was lowest (1.2%) in patients with a score of 0 (n = 61) and highest (31.9%) in patients with a score of 5 (n = 15, p < 0.001).

Conclusion

High resting CFV is associated with worse survival in ischemic and nonischemic HF with reduced EF. The value is independent and additive to resting EF, CFVR, LVCR, and inducible ischemia.
Effects of women with gestational diabetes mellitus related weight gain on pregnancy outcomes and its experiences in weight management programs: a mixed-methods systematic review
Authors: He Jing , Hu Kaili , Wang Binghua , Wang Hui
Published: November 2023

Introduction

Proper controlling gestational diabetes mellitus (GDM)-related gestational weight gain (GWG) during pregnancy can optimize pregnancy outcomes and improve postpartum glucose homeostasis. This study aimed to explore the existing intervention programs, the effects on pregnancy outcomes, and the experiences of weight management for GDM-related GWG in women with GDM.

Methods

This mixed-methods systematic review was retrieved from nine databases. The retrieval time was from the database construction to September 20, 2023, and all studies were published in English and Chinese. The included records used quantitative, qualitative, or mixed methods and reported original studies of weight-related intervention regimens, effects on pregnancy outcomes, and women’s experiences and perceptions. This review used a convergent segregated approach to synthesize and integrate research findings from Joanna Briggs Institute (JBI) mixed-methods systematic reviews.

Results

There were 16 articles that met the inclusion criteria, and the articles came from seven different countries and included 23,997 women with GDM. The meta-analysis pooled outcomes for the incidence of weight gain exceeding the Institute of Medicine (IOM) recommendations after GDM diagnosis to delivery was 0.31% (95% CI 0.21-0.42). The effectiveness of GDM-related weight interventions in reducing weight gain after GDM diagnosis was supported by quantitative evidence. The GDM-related GWG below the IOM recommendations is a protective factor (OR=0.68, 95%CI 0.48-0.97) for large for gestational Age (LGA), and above the IOM recommendations is a risk factor (OR=1.62, 95%CI 1.15-2.27) for LGA. In addition, no significant statistical significance was found in the pooled outcomes of small for gestational age (SGA). Avoiding excessive weight gain helps to optimize neonatal birth weight, pregnancy outcomes, and maternal blood glucose levels. According to qualitative survey results, some women with GDM experienced weight stigma, and a positive relationship between healthcare providers and GDM women helped in weight management.

Conclusion

Following a diagnosis of GDM, weight management interventions positively affected GWG and pregnancy outcomes. In order to improve compliance and safety of weight management in women with GDM, criteria and interventions for weight gain associated with GDM need to be further explored and improved.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=404492, identifier CRD42023404492.
Immigration and C-sections incidence: Maternal care and perinatal outcomes in the context of the pandemic in Chile
Authors: Carroza Escobar María Begoña , Silva Nicole , Ortíz-Contreras Jovita , Villegas Rodrigo , Vargas Sergio L. , Núñez Claudio , Vergara Maldonado Luis Felipe , Villanueva Loreto Paola
Published: November 2023

Introduction

Immigration has increased significantly in Chile. Despite that all pregnant women, regardless of nationality and immigration status, have the right to access to all healthcare services during pregnancy, childbirth, and postpartum, inequities in health care outcomes and health provision have been reported. During COVID-19 pandemic, these inequities are completely unknown.

Objective

The aim of this study was to compare the incidence of c-sections according to mother's migration status, as well as other maternal care and perinatal outcomes in women giving birth at San José Hospital in Santiago, Chile, during the COVID-19 pandemic.

Methods

A retrospective cohort study was designed including 10,166 registered single births at the San José Hospital between March 2020 and August 2021. To compare between groups, statistical tests such as Chi-square and Fisher's exact were used. Log Binomial regression models were performed adjusted for potential confounding variables. To estimate the strength of association the relative risk was used.

Results

Immigrant mothers account for 48.1% of the registered births. Compared to non-immigrant women, immigrants exhibit a higher proportion of c-section, specifically, emergency c-section (28.64% vs. 21.10%; p-value < 0.001) but a lower proportion of and having a preterm birth (8.24% vs. 13.45%; p < 0.05), receiving personalized childbirth care (13.02% vs. 14.60%; p-value < 0.05), companion during labor and childbirth (77.1% vs. 86.95%; p-value < 0.001), And postpartum attachment to newborn (73% vs. 79.50%; p-value < 0.001). The proportion of COVID exposure was not significant between groups, not the severity also. Haitians had a highest risk of undergoing emergency c-section (aRR = 1.61) and Venezuelans had a highest risk of elective c-section (aRR = 2.18) compared to non-immigrants.

Conclusion

This study reports high rates of c-sections in the entire population, but in immigrant populations it is even higher. Additionally, it found gaps in maternal care and perinatal outcomes between immigrants and non-immigrants. More studies are needed to elucidate the possible causes of these differences and establish new regulations to protect the reproductive rights of the immigrant population.
The two-stage molecular scenery of SARS-CoV-2 infection with implications to disease severity: An in-silico quest
Authors: Potamias George , Gkoublia Polymnia , Kanterakis Alexandros
Published: November 2023

Introduction

The two-stage molecular profile of the progression of SARS-CoV-2 (SCOV2) infection is explored in terms of five key biological/clinical questions: (a) does SCOV2 exhibits a two-stage infection profile? (b) SARS-CoV-1 (SCOV1) vs. SCOV2: do they differ? (c) does and how SCOV2 differs from Influenza/INFL infection? (d) does low viral-load and (e) does COVID-19 early host response relate to the two-stage SCOV2 infection profile? We provide positive answers to the above questions by analyzing the time-series gene-expression profiles of preserved cell-lines infected with SCOV1/2 or, the gene-expression profiles of infected individuals with different viral-loads levels and different host-response phenotypes.

Methods

Our analytical methodology follows an in-silico quest organized around an elaborate multi-step analysis pipeline including: (a) utilization of fifteen gene-expression datasets from NCBI’s gene expression omnibus/GEO repository; (b) thorough designation of SCOV1/2 and INFL progression stages and COVID-19 phenotypes; (c) identification of differentially expressed genes (DEGs) and enriched biological processes and pathways that contrast and differentiate between different infection stages and phenotypes; (d) employment of a graph-based clustering process for the induction of coherent groups of networked genes as the representative core molecular fingerprints that characterize the different SCOV2 progression stages and the different COVID-19 phenotypes. In addition, relying on a sensibly selected set of induced fingerprint genes and following a Machine Learning approach, we devised and assessed the performance of different classifier models for the differentiation of acute respiratory illness/ARI caused by SCOV2 or other infections (diagnostic classifiers), as well as for the prediction of COVID-19 disease severity (prognostic classifiers), with quite encouraging results.

Results

The central finding of our experiments demonstrates the down-regulation of type-I interferon genes (IFN-1), interferon induced genes (ISGs) and fundamental innate immune and defense biological processes and molecular pathways during the early SCOV2 infection stages, with the inverse to hold during the later ones. It is highlighted that upregulation of these genes and pathways early after infection may prove beneficial in preventing subsequent uncontrolled hyperinflammatory and potentially lethal events.

Discussion

The basic aim of our study was to utilize in an intuitive, efficient and productive way the most relevant and state-of-the-art bioinformatics methods to reveal the core molecular mechanisms which govern the progression of SCOV2 infection and the different COVID-19 phenotypes.
Raman-dielectrophoresis goes viral: towards a rapid and label-free platform for plant virus characterization
Authors: Sacco Alessio , Barzan Giulia , Matić Slavica , Giovannozzi Andrea M. , Rossi Andrea M. , D’Errico Chiara , Vallino Marta , Ciuffo Marina , Noris Emanuela , Portesi Chiara
Published: November 2023

An innovative spectroscopic method that allows to chemically and structurally characterize viruses directly in suspension within few minutes was developed. A library of five different plant viruses was obtained combining dielectrophoresis (DEP), performed with a device specifically designed to capture and agglomerate virus particles, and Raman spectroscopy to provide a chemical fingerprint of virions. The tested viruses, purified from infected plants, were chosen for their economic impact on horticultural crops and for their different morphological and structural features. Using the Raman-DEP device, specific profiles for each virus were successfully obtained, relying on chemical differences occurring even with genetically similar viruses belonging to the same taxonomic species and morphologically indiscernible by transmission electron microscopy (TEM). Moreover, we investigated the potentiality of Raman-DEP to follow dynamic changes occurring upon heat treatment of tobacco mosaic virus (TMV) particles. Raman peak deviations linked to TMV coat protein conformation were observed upon treatment at temperatures equal or higher than 85°C, substantiating the rod-to-spherical shape transitions observed by TEM and the concomitant drastic loss of infectivity following plant inoculation. Overall, the Raman-DEP method can be useful for the characterization of virus (nano)particles, setting the basis to create a database suitable for the study of viruses or virus derived-nanoparticles relevant for the agricultural, medical, or biotechnological fields.
A rapid colloidal gold immunochromatographic assay based on polyclonal antibodies against HtpsC protein for the detection of Streptococcus suis
Authors: Lu Yawei , Wang Sibo , Cai Xushen , Cao Min , Lu Qingyu , Hu Dan , Chen Qiong , Xiong Xiaohui
Published: November 2023

An efficient and rapid immunochromatographic assay (ICA) has been engineered for the detection of Streptococcus suis (S. suis). The underpinning principle of this ICA test lies in the use of polyclonal antibodies (pAbs) decorated with colloidal gold, which are specific to S. suis. These pAbs were derived from rabbits immunized with type II histidine triad protein (HtpsC) and HtpsC-N of S. suis. The sensitivity of the ICA was noteworthy, identifying S. suis at bacterial concentrations as diminutive as 1.0 × 103 CFU/mL. Moreover, the assay demonstrated respectable specificity and did not indicate false positives for other bacterial species (Escherichia coli, Salmonella, Staphylococcus aureus, Listeria monocytogenes, Streptococcus pyogenes, Streptococcus lactis, or Enterococcus faecalis). The assay was also capable of detecting multiple S. suis serotypes containing the htpsC gene, including serotypes 1–9, 12, 14, 16 and 23. Nonetheless, the detection of S. suis that lacks the htpsC gene remained beyond the capabilities of this assay. A simultaneous analysis of 16 samples utilizing PCR substantiated the reliability of the ICA test. The assay’s results can be procured within a 15-min window, making it a suitable option for field application. Broadly, this study underscores the potential of the HtpsC protein as a target antigen for the detection of S. suis, and proposes that the HtpsC protein be evaluated further in other detection assays specific for S. suis.
Non-pharmacological interventions for improving sleep in people living with HIV: a systematic narrative review
Authors: Meng Jingjing , Zheng Chunyuan , Wang Honghong , Välimäki Maritta , Wang Min
Published: November 2023

Background

Sleep disturbances are common in people living with Human Immunodeficiency Virus (HIV) and may lead to poor adherence to antiretroviral therapy and worsen HIV symptom severity. Due to the side effects of pharmacotherapy for sleep disturbances, there is more room for non-pharmacological interventions, but knowledge of how these non-pharmacological interventions have been used to improve sleep in people living with HIV (PLWH) is still missing.

Objective

To investigate the content of non-pharmacological interventions, sleep measurements, and the impact of these interventions on improving sleep in PLWH.

Methods

Following PRISMA guidelines, we conducted a systematic search on PubMed, EMBASE, Cochrane Central Registry of Controlled Trials, Cumulative Index to Nursing and Allied Health Literature, Web of Science, China National Knowledge Infrastructure, Wanfang Data, and China Biology Medicine disc. Non-pharmacological interventions for improving sleep in PLWH were included, and study quality was assessed using the Joanna Briggs Institute (JBI) critical appraisal checklists. We performed a narrative approach to synthesize the data to better understand the details and complexity of the interventions.

Results

Fifteen experimental studies in three categories for improving sleep in PLWH were included finally, including psychological interventions (components of cognitive-behavioral therapy for insomnia or mindfulness-based cognitive therapy, n = 6), physical interventions (auricular plaster therapy, acupuncture, and exercise, n = 8), and elemental interventions (speed of processing training with transcranial direct current stimulation, n = 1). Wrist actigraphy, sleep diary, and self-reported scales were used to measure sleep. Psychological interventions and physical interventions were found to have short-term effects on HIV-related sleep disturbances.

Conclusions

Psychological and physical interventions of non-pharmacological interventions can potentially improve sleep in PLWH, and the combination of patient-reported outcomes and actigraphy devices can help measure sleep comprehensively. Future non-pharmacological interventions need to follow protocols with evidence-based dosing, contents, and measures to ensure their sustainable and significant effects.
Feeding soy protein concentrates with low or high isoflavone decreases liver inflammation by reducing lipopolysaccharide translocation
Authors: Li Wei , Hakkak Reza
Published: November 2023

Lipopolysaccharide (LPS) translocation and inflammation contribute to the increased risk of chronic diseases, including non-alcoholic fatty liver disease (NAFLD), associated with obesity. Previously, we reported that feeding soy protein with high or low (negligible) isoflavone reduces liver steatosis in obese Zucker rats, and the reduced steatosis is accompanied by decreased serum C-reactive protein levels. The current study investigated the effect of feeding soy protein concentrate (SPC) with high or low isoflavone (HIF or LIF) on liver inflammation and LPS translocation in obese Zucker rats. Six-week-old male lean (L, n = 21) and obese (O, n = 21) Zucker rats were fed casein control, SPC-LIF, or SPC-HIF diets for 18 weeks. At the end of 18 weeks, the expression levels of tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), inducible nitric oxide synthase (iNOS), arginase 1 (ARG1), lipopolysaccharide binding protein (LBP), myeloperoxidase (MPO), and sterol regulatory element-binding protein 1 (SREBP-1) were significantly higher in obese rats compared to lean rats. Compared to the casein control diet, both the SPC-LIF and SPC-HIF diets significantly decreased TNF-α, MCP-1, iNOS, and LBP expression in obese rats, which is accompanied by significantly less LPS staining in liver slides from SPC-LIF-and SPC-HIF-fed obese rats compared to the casein control diet-fed obese rats. Taken together, the SPC-LIF and SPC-HIF diets attenuated liver inflammation in obese Zucker rats, likely by decreasing LPS translocation.