bluuebunny/crossref_metadata_2025 huggingface dataset:
Eco-Sim: A Parametric Tool to Evaluate the Environmental and Economic Feasibility of Decentralized Energy Systems
Authors: Siraganyan Karni , Perera Amarasinghage Tharindu Dasun , Scartezzini Jean-Louis , Mauree Dasaraden
Published: February 2019

Due to climate change and the need to decrease the carbon footprint of urban areas, there is an increasing pressure to integrate renewable energy and other components in urban energy systems. Most of the models or available tools do not provide both an economic and environmental assessment of the energy systems and thus lead to the design of systems that are sub-optimal. A flexible and modular simulation tool, Eco-Sim, is thus developed in the current study to conduct a comprehensive techno-economic and environmental assessment of a distributed energy system considering different configuration scenarios. Subsequently, an intermodel comparison is conducted with the Hybrid Optimization Model for Electric Renewable (HOMER) Pro as well as with a state-of-the-art industrial tool. Eco-Sim is then extended by including the heating demand, thermal conversion (by using heat pumps and solar thermal) methods and thermal storage. A parametric analysis is conducted by considering different capacities of solar photovoltaics (PV), solar thermal panels and energy storage technologies. The levelized cost of electricity, the autonomy level and the CO 2 emissions are used as the key performance indicators. Based on the analysis of a study case conducted in a neighbourhood in Geneva, Switzerland, the study reveals that, with the present market prices for batteries and seasonal changes in solar energy potential, the combination of solar PV with battery storage doesn’t bring a significant autonomy to the system and increases the CO 2 emissions of the system. However, the integration of thermal storage and solar thermal generation is shown to considerably increase the autonomy of the neighbourhood. Finally, multiple scenarios are also run in order to evaluate the sensitivity of economic parameters on the performance indicators of the system. Under the assumptions of the model, to foster investments in solar PV and battery installations, falling installation costs or stronger policies in favor of renewable energy seem necessary for the future.
A Method for the Simultaneous Suppression of DC Capacitor Fluctuations and Common-Mode Voltage in a Five-Level NPC/H Bridge Inverter
Authors: Wu Ming , Song Zhenhao , Lv Zhipeng , Zhou Kai , Cui Qi
Published: February 2019

To suppress the direct current (DC) capacitor voltage fluctuations and the common-mode voltage (CMV) in a three-phase, five-level, neutral-point-clamped (NPC)/H-bridge inverter, this paper analyzes the influence of all voltage vectors on the neutral point potential of each phase under different pulse mappings in detail with an explanation of the CMV distribution. Then, based on the traditional space vector pulse width modulation (SVPWM) algorithm, a dual-pulse-mapping algorithm is proposed to suppress the DC capacitor fluctuations and the CMV simultaneously. In the algorithm, the reference voltage synthesis selects the voltage vector that has the smallest CMV value as the priority. In addition, the two kinds of pulse mappings that have opposite effects on the neutral point potential are switched to output. At the same time, regulating factors are introduced to adjust the working time of each voltage vector under the two pulse mappings; then, the capacitor voltages can be balanced. Both the simulation and experiment demonstrate the algorithm’s effectiveness.
Experimental Study of Profile Control with Foam Stabilized by Clay Particle and Surfactant
Authors: Li Songyan , Qiao Chenyu , Ji Guowei , Wang Qun , Tao Lei
Published: February 2019

Foam is a kind of ideal fluid for profile control in petroleum engineering, which has attracted intense interests of scholars globally in recent years. In this study, a foam system stabilized with anionic surfactants and clay particles was proposed for profile control in reservoirs, and the formulation was optimized experimentally. Moreover, flooding experiments in visible porous media models and in sandpacks were conducted to test the plugging effect of the foam system on reservoirs, and the effects of different factors such as gas–liquid ratio, temperature and permeability on profile control were also evaluated. According to the experimental results, the clay-HY-2 system was elected for its satisfactory foamability, stability, and salinity resistance, and the optimum concentrations of HY-2 and clay particle are 0.6 wt% and 5.0 wt%, respectively. Compared with traditional foam fluids, the clay-HY-2 system can form denser and smaller bubbles in high- and middle-permeable layers, enhancing the plugging effect there, while there are less bubbles in low-permeable layers, i.e., the restriction on the flow in narrow structures is slight. The clay-HY-2 foam can perform the efficient and uniform profile control effect on sandpacks when the foam quality is around 50%. The resistance factor of the foam decrease gradually with the increasing temperature, however, the resistance factor remains higher than 350.0 when the temperature reaches 80.0 °C. When the permeability exceeds 1502.0 mD, the clay-HY-2 foam can perform deep profile control in reservoirs, and the resistance factor are not sensitive to the change of permeability when it exceeds 3038.0 mD. Besides, the site application case shows that the clay-HY-2 foam do have good profile control effect on reservoirs, i.e., improving oil production and declining water cut.
Performance Degradation of Surface PMSMs with Demagnetization Defect under Predictive Current Control
Authors: Verkroost Lynn , Druant Joachim , Vansompel Hendrik , De Belie Frederik , Sergeant Peter
Published: February 2019

To control the current of a surface mounted permanent magnet synchronous machine fed by a two-level voltage source inverter, a large variety of control algorithms exists. Each of these controllers performs differently concerning dynamic performance and control- and voltage quality, but also concerning sensitivity to demagnetization faults. Therefore, this paper investigates the performance degradation of three advanced predictive controllers under a partial demagnetization fault. The three predictive controllers are: finite-set model based predictive control, deadbeat control, and a combination of both previous algorithms. To achieve this goal, the three predictive controllers are first compared under healthy conditions, and afterwards under a partial demagnetization fault. A PI controller is added to the comparison in order to provide a model-independent benchmark. Key performance indicators, obtained from both simulations and experimental results on a 4 kW axial flux permanent magnet synchronous machine with yokeless and segmented armature topology, are introduced to enable a quantification of the performance degradation of the controllers under a demagnetization fault. A general conclusion is that the deadbeat controller shows superior control quality, even under partial demagnetization.
Fast and Accurate Model of Interior Permanent-Magnet Machine for Dynamic Characterization
Authors: Drobnič Klemen , Gašparin Lovrenc , Fišer Rastko
Published: February 2019

A high-fidelity two-axis model of an interior permanent-magnet synchronous machine (IPM) presents a convenient way for the characterization and validation of motor dynamic performance during the design stage. In order to consider a nonlinear IPM nature, the model is parameterized with a standard dataset calculated beforehand by finite-element analysis. From two possible model implementations, the current model (CM) seems to be preferable to the flux-linkage model (FLM). A particular reason for this state of affairs is the rather complex and time-demanding parameterization of FLM in comparison with CM. For this reason, a procedure for the fast and reliable parameterization of FLM is presented. The proposed procedure is significantly faster than comparable methods, hence providing considerable improvement in terms of computational time. Additionally, the execution time of FLM was demonstrated to be up to 20% shorter in comparison to CM. Therefore, the FLM should be used in computationally intensive simulation scenarios that have a significant number of iterations, or excessive real-time time span.
Comparative Life Cycle Assessment of HTC Concepts Valorizing Sewage Sludge for Energetic and Agricultural Use
Authors: Meisel Kathleen , Clemens Andreas , Fühner Christoph , Breulmann Marc , Majer Stefan , Thrän Daniela
Published: February 2019

In many countries, sewage sludge is directly used for energy and agricultural purposes after dewatering or digestion and dewatering. In recent years, there has been a growing interest in additional upstream hydrothermal carbonization (HTC), which could lead to higher yields in the energetic and agricultural use. Twelve energetic and agricultural valorization concepts of sewage sludge are defined and assessed for Germany to investigate whether the integration of HTC will have a positive effect on the greenhouse gas (GHG) emissions. The study shows that the higher expenses within the HTC process cannot be compensated by additional energy production and agricultural yields. However, the optimization of the HTC process chain through integrated sewage sludge digestion and process water recirculation leads to significant reductions in GHG emissions of the HTC concepts. Subsequently, nearly the same results can be achieved when compared to the direct energetic use of sewage sludge; in the agricultural valorization, the optimized HTC concept would be even the best concept if the direct use of sewage sludge will no longer be permitted in Germany from 2029/2032. Nevertheless, the agricultural valorization concepts are not generally advantageous when compared to the energetic valorization concepts, as it is shown for two concepts.
Integration of Marine Wave Energy Converters into Seaports: A Case Study in the Port of Valencia
Authors: Cascajo Raúl , García Emilio , Quiles Eduardo , Correcher Antonio , Morant Francisco
Published: February 2019

A feasibility study for the installation of Wave Energy Converters (WEC) in a Spanish Mediterranean port is evaluated in this paper. The final aim is to evaluate the possibility of building a new infrastructure which combines a breakwater and a WEC able to provide energy to the commercial port of Valencia. An estimation of the wave power potential is made according to existing databases from different sources. A review of the existing WEC types is carried out in order to choose the most suitable technology for its installation in a port environment. The authors discuss the main advantages and issues of the integration of WEC in port breakwaters. A prospective study for the Port of Valencia is made, considering the port energy demand evolution, historical data on wave energy potential and the port expansion plans. We conclude that Overtopping Devices (OTDs) are the most suitable ones to allow the good integration with the new breakwater needed for the expansion of the Port of Valencia and we give an estimation on the power available from the resource in our case study.
Evaluation of Slope Stability Considering the Preservation of the General Patrimonial Cemetery of Guayaquil, Ecuador
Authors: Morante Fernando , Aguilar Maribel , Ramírez Gonzalo , Blanco Roberto , Carrión Paúl , Briones Josué , Berrezueta Edgar
Published: February 2019

Inefficient blasting techniques and poor closure management of the old quarry that existed during the 1970s in the area of the actual General Patrimonial Cemetery of Guayaquil resulted in an almost vertical slope of approximately 50 m in height and an intense induced fracturing that weakened the rock mass. This led to the loss of stability and increased material detachment, which damaged the infrastructure of the graveyard representing a risk to visitors and workers. The aim of this research is to evaluate the slope stability through a geotechnical analysis that allows decision-making to recover and preserve the safety of the area. In this work, we used structural measurements and observations made in the field, as well as a three-dimensional model of the slant generated by photographs taken by a drone. Slope Mass Rating (SMR) and Chinese Slope Mass Rating (CSMR) rankings were used to evaluate stability, susceptibility to rockfall was determined using a theoretical–practical procedure, and the safety factor was calculated using the Morgenstern–Price method. The analysis of the geomechanical classifications yielded a low stability value of the slope, which was in accordance with the high susceptibility to rockfall and with the low safety factor. Based on these results, we recommend the application of systematic bolt and shotcrete for stabilization.
Associations Between Socio-Economic Status and Child Health: Findings of a Large German Cohort Study
Authors: Poulain Tanja , Vogel Mandy , Sobek Carolin , Hilbert Anja , Körner Antje , Kiess Wieland
Published: February 2019

The familial social background of a child can significantly impact their behavior and health. We investigated associations between socio-economic status (SES) and health parameters and behaviors in German children and adolescents. Data were collected between 2011 and 2018 in the framework of the LIFE Child study. Participants included 2998 children aged 3–18 years. SES was represented by an index combining information on parental education, occupation, and income. Associations between SES and health outcomes were estimated using linear and logistic regression analyses. In a moderator analysis, all associations were checked for interactions between SES and age or sex. A higher SES composite score was associated with better health (lower body mass index (β = −0.26), fewer behavioral difficulties (β = −0.18), higher quality of life (β = 0.21), fewer critical life events (odds ratio (OR) = 0.93); all p < 0.05) and a healthier lifestyle (healthier nutrition (β = 0.16), less excessive television use (OR = 0.87), less nicotine consumption (OR = 0.93), and more physical activity (OR = 1.18); all p < 0.05). However, SES was not associated with alcohol consumption (OR = 1.02) or sleep problems (β = −0.04). The strengths of the associations between SES and child health did not differ depending on SES indicator (education, occupation, income). The associations between SES and parent-reported behavioral difficulties and physical activity were stronger in older vs. younger children. In contrast, none of the observed associations were moderated by sex. This study highlights the strong association between socio-economic status and child health, even in modern Western societies.
Adsorption Thermodynamics and Dynamics of Three Typical Dyes onto Bio-adsorbent Spent Substrate of Pleurotus eryngii
Authors: Wu Jianguo , Xia Aiqiang , Chen Chunyan , Feng Liuying , Su Xiaohui , Wang Xinfeng
Published: February 2019

Dyeing wastewater is very hard to treat, and adsorption could be a good choice. Spent substrate of Pleurotus eryngii (SSPE) was first used to adsorb malachite green, safranine T and methylene blue from aqueous solutions, and the corresponding adsorption isotherm, thermodynamics and dynamics models were simulated. More than 93% of the dyes were removed with solutions with 100 mg/L of initial dye concentration, 1 g of SSPE and pH of 6.0 after adsorption for 4 h. Freundlich isotherm models fit better the adsorption data than Langmuir models. Adsorption of the dyes onto SSPE was a spontaneous exothermic process based on an adsorption thermodynamics model. SSPE could adsorb the dyes rapidly, and a second-order kinetics model fit better with the adsorption data than a pseudo first-order kinetics model. Accordingly, SSPE could be a good bio-adsorbent for the removal of malachite green, safranine T and methylene blue from the aqueous solution.
Severe Hypoglycemia as a Predictor of End-Stage Renal Disease in Type 2 Diabetes: A National Cohort Study
Authors: Lee Yu-Li , Yen Shih-Jung , Shin Shyi-Jang , Huang Yi-Chi , He Jiun Shiuan , Lin Kun-Der
Published: February 2019

Aims: This study investigated whether there is a link between severe hypoglycemia and progression into end-stage renal disease (ESRD) in patients with type 2 diabetes. Methods: Tapping into Taiwan’s Health Insurance Research Database, we identified all type 2 diabetes patients between 1996 and 2013 and identified those diagnosed with a severe hypoglycemia episode during an emergency department visit and those who were not. Controls were then matched 1:1 for age, sex, index year, and medication. Results: We identified 468,421 type 2 diabetes patients diagnosed as having severe hypoglycemia in an emergency department visit. Compared with controls, these patients with SH had a higher risk of all-cause mortality (Hazard Ratio (HR), 1.76; 95% confidence interval, 1.61–1.94) and progressed into ESRD within a shorter period of time. Results were similar after controlling for competing risk. Conclusion: Severe hypoglycemia is significantly associated with worsening renal dysfunction in patients with type 2 diabetes and hastened progression into ESRD.
Surface Modification of PVDF Membranes for Treating Produced Waters by Direct Contact Membrane Distillation
Authors: Kamaz Mohanad , Sengupta Arijit , Gutierrez Ashley , Chiao Yu-Hsuan , Wickramasinghe Ranil
Published: February 2019

Direct contact membrane distillation (DCMD) has been conducted to treat hydraulic fracturing-produced water using polyvinylidenedifluoride (PVDF) membranes. Tailoring the surface properties of the membrane is critical in order to reduce the rate of adsorption of dissolved organic species as well as mineral salts. The PVDF membranes have been modified by grafting zwitterion and polyionic liquid-based polymer chains. In addition, surface oxidation of the PVDF membrane has been conducted using KMnO4 and NaOH. Surface modification conditions were chosen in order to minimize the decrease in contact angle. Thus, the membranes remain hydrophobic, essential for suppression of wetting. DCMD was conducted using the base PVDF membrane as well as modified membranes. In addition, DCMD was conducted on the base membrane using produced water (PW) that was pretreated by electrocoagulation to remove dissolved organic compounds. After DCMD all membranes were analyzed by scanning electron microscopy imaging as well as Energy-Dispersive X-Ray spectroscopy. Surface modification led to a greater volume of PW being treated by the membrane prior to drastic flux decline. The results indicate that tailoring the surface properties of the membrane enhances fouling resistance and could reduce pretreatment requirements.
Accelerometer-Measured Physical Activity in Children and Adolescents at Altitudes over 3500 Meters: A Cross-Sectional Study in Tibet
Authors: Nie Ming-jian , Fan Chao-qun , Sun Rui-zhe , Wang Jing-jing , Feng Qiang , Zhang Yan-feng , Yao Zhi , Wang Mei
Published: February 2019

There is a scarcity of studies on the physical activity (PA) of children and adolescents who live at high altitudes. This study aimed to objectively assess PA of children and adolescents living in the Tibet at altitudes over 3500 m and to examine its difference by ethnicity, gender, age/grade, and body weight status groups. A sample of 397 students aged 9–18 years were recruited from 7 schools in Lhasa, Tibet. PA was measured using accelerometers (ActiGraph GT3X) for seven consecutive days and moderate to vigorous PA (MVPA) was identified using the Evenson (2008) cut-points. Participant MVPA was 62.3 min/day, with 65.5 min/day during weekdays and 54.1 min/day on weekends. Indigenous Tibetans were more active than Hans, and boys had more MVPA than girls. Age had a significantly weak negative correlation with MVPA. There was no significant difference in MVPA between the non-overweight and overweight/obese groups. Overall, only 9.1% (13.8% in boys and 4.5% in girls) accumulated at least 60 min of MVPA per day. Compared to their counterparts in other regions, the daily MVPA of children and adolescents living on the Tibetan Plateau at altitudes over 3500 m was relatively high. However, the proportion of meeting the WHO’s PA recommendations was extremely low.
Effects of Climate Change and Heterogeneity of Local Climates on the Development of Malaria Parasite (Plasmodium vivax) in Moscow Megacity Region
Authors: Mironova Varvara , Shartova Natalia , Beljaev Andrei , Varentsov Mikhail , Grishchenko Mikhail
Published: February 2019

The article presents the results of a spatio-temporal analysis of the changes of the favorability of climatic conditions for the transmission of vivax malaria in the Moscow megacity and its surroundings during the period from 1977 to 2016. Using the historical temperature records at urban and rural weather stations, we calculated the key indicators of climate favorability for malaria transmission, viz. the sum of effective temperatures, the duration of the season of effective infectiveness, and a new integral index of climate favorability. We demonstrated a dramatic increase of all three indicators, which accelerated after 1984, and a high spatial heterogeneity among them. Due to the urban heat island effect, the degree of climatic favorability is especially high in the densely urbanized areas of Moscow megacity compared with the suburban and rural areas. Climatic conditions for vivax malaria in Moscow are better now than before. The season of effective infectiveness continues in the central part of the city for 25 days longer, and the integral index of climate favorability is 85% higher in comparison to mean values over the rural surroundings. The study contains an alert regarding the risk of malaria resurgence in the Moscow region in the case of the sufficient importation of cases from abroad.
A Novel Process-Oriented Graph Storage for Dynamic Geographic Phenomena
Authors: Xue Cunjin , Wu Chengbin , Liu Jingyi , Su Fenzhen
Published: February 2019

There exists a sort of dynamic geographic phenomenon in the real world that has a property which is maintained from production through development to death. Using traditional storage units, e.g., point, line, and polygon, researchers face great challenges in exploring the spatial evolution of dynamic phenomena during their lifespan. Thus, this paper proposes a process-oriented two-tier graph model named PoTGM to store the dynamic geographic phenomena. The core ideas of PoTGM are as follows. 1) A dynamic geographic phenomenon is abstracted into a process with a property that is maintained from production through development to death. A process consists of evolution sequences which include instantaneous states. 2) PoTGM integrates a process graph and a sequence graph using a node–edge structure, in which there are four types of nodes, i.e., a process node, a sequence node, a state node, and a linked node, as well as two types of edges, i.e., an including edge and an evolution edge. 3) A node stores an object, i.e., a process object, a sequence object, or a state object, and an edge stores a relationship, i.e., an including or evolution relationship between two objects. Experiments on simulated datasets are used to demonstrate an at least one order of magnitude advantage of PoTGM in relation to relationship querying and to compare it with the Oracle spatial database. The applications on the sea surface temperature remote sensing products in the Pacific Ocean show that PoTGM can effectively explore marine objects as well as spatial evolution, and these behaviors may provide new references for global change research.
Impacts of Silver Nanoparticles on Plants: A Focus on the Phytotoxicity and Underlying Mechanism
Authors: Yan An , Chen Zhong
Published: February 2019

Nanotechnology was well developed during past decades and implemented in a broad range of industrial applications, which led to an inevitable release of nanomaterials into the environment and ecosystem. Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials in various fields, especially in the agricultural sector. Plants are the basic component of the ecosystem and the most important source of food for mankind; therefore, understanding the impacts of AgNPs on plant growth and development is crucial for the evaluation of potential environmental risks on food safety and human health imposed by AgNPs. The present review summarizes uptake, translocation, and accumulation of AgNPs in plants, and exemplifies the phytotoxicity of AgNPs on plants at morphological, physiological, cellular, and molecular levels. It also focuses on the current understanding of phytotoxicity mechanisms via which AgNPs exert their toxicity on plants. In addition, the tolerance mechanisms underlying survival strategy that plants adopt to cope with adverse effects of AgNPs are discussed.
Aggrecan Hypomorphism Compromises Articular Cartilage Biomechanical Properties and Is Associated with Increased Incidence of Spontaneous Osteoarthritis
Authors: Alberton Paolo , Dugonitsch Hans Christian , Hartmann Bastian , Li Ping , Farkas Zsuzsanna , Saller Maximilian Michael , Clausen-Schaumann Hauke , Aszodi Attila
Published: February 2019

The gene encoding the proteoglycan aggrecan (Agc1) is abundantly expressed in cartilage during development and adulthood, and the loss or diminished deposition of the protein results in a wide range of skeletal malformations. Furthermore, aggrecan degradation is a hallmark of cartilage degeneration occurring in osteoarthritis. In the present study, we investigated the consequences of a partial loss of aggrecan in the postnatal skeleton and in the articular cartilage of adult mice. We took advantage of the previously described Agc1tm(IRES-CreERT2) mouse line, which allows for conditional and timely-regulated deletion of floxed, cartilage-expressed genes. As previously reported, the introduction of the CreERT2 cassette in the 3’UTR causes a disruption of the normal expression of Agc1 resulting in a hypomorphic deposition of the protein. In homozygous mice, we observed a dwarf phenotype, which persisted throughout adulthood supporting the evidence that reduced aggrecan amount impairs skeletal growth. Homozygous mice exhibited reduced proteoglycan staining of the articular cartilage at 6 and 12 months of age, increased stiffening of the extracellular matrix at six months, and developed severe cartilage erosion by 12 months. The osteoarthritis in the hypomorph mice was not accompanied by increased expression of catabolic enzymes and matrix degradation neoepitopes. These findings suggest that the degeneration found in homozygous mice is likely due to the compromised mechanical properties of the cartilage tissue upon aggrecan reduction.
Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies
Authors: Monnier Noadya , Furlan Aurélien L. , Buchoux Sébastien , Deleu Magali , Dauchez Manuel , Rippa Sonia , Sarazin Catherine
Published: February 2019

Rhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the molecular scale. Representative models were constructed and the interaction with RLs was studied by Fourier transform infrared (FTIR) and deuterium nuclear magnetic resonance (2H NMR) spectroscopic measurements. Molecular dynamic (MD) simulations were performed to investigate RL insertion in lipid bilayers. Our results showed that the RLs fit into the membrane models and were located near the lipid phosphate group of the phospholipid bilayers, nearby phospholipid glycerol backbones. The results obtained with plant plasma membrane models suggest that the insertion of RLs inside the lipid bilayer did not significantly affect lipid dynamics. Oppositely, a clear fluidity increase of fungi membrane models was observed. This effect was related to the presence and the specific structure of ergosterol. The nature of the phytosterols could also influence the RL effect on plant plasma membrane destabilization. Subtle changes in lipid dynamics could then be linked with plant defense induction and the more drastic effects associated with fungal membrane destabilization.
An RNA Switch of a Large Exon of Ninein Is Regulated by the Neural Stem Cell Specific-RNA Binding Protein, Qki5
Authors: Hayakawa-Yano Yoshika , Yano Masato
Published: February 2019

A set of tissue-specific splicing factors are thought to govern alternative splicing events during neural progenitor cell (NPC)-to-neuron transition by regulating neuron-specific exons. Here, we propose one such factor, RNA-binding protein Quaking 5 (Qki5), which is specifically expressed in the early embryonic neural stem cells. We performed mRNA-SEQ (Sequence) analysis using mRNAs obtained by developing cerebral cortices in Qk (Quaking) conditional knockout (cKO) mice. As expected, we found a large number of alternative splicing changes between control and conditional knockouts relative to changes in transcript levels. DAVID (The Database for Annotation, Visualization and Integrated Discovery) and Metascape analyses suggested that the affected spliced genes are involved in axon development and microtubule-based processes. Among these, the mRNA coding for the Ninein protein is listed as one of Qki protein-dependent alternative splicing targets. Interestingly, this exon encodes a very long polypeptide (2121 nt), and has been previously defined as a dynamic RNA switch during the NPC-to-neuron transition. Additionally, we validated that the regulation of this large exon is consistent with the Qki5-dependent alternative exon inclusion mode suggested by our previous Qki5 HITS-CLIP (high throughput sequencing-cross linking immunoprecipitation) analysis. Taken together, these data suggest that Qki5 is an important factor for alternative splicing in the NPC-to-neuron transition.
Comparative Transcriptome Analysis Reveals the Transcriptional Alterations in Growth- and Development-Related Genes in Sweet Potato Plants Infected and Non-Infected by SPFMV, SPV2, and SPVG
Authors: Shi Jiang , Zhao Lin , Yan Baiyuan , Zhu Yueqing , Ma Huasheng , Chen Wenyue , Ruan Songlin
Published: February 2019

Field co-infection of multiple viruses results in considerable losses in the yield and quality of storage roots in sweet potato. However, little is known about the molecular mechanisms underlying developmental disorders of sweet potato subjected to co-infection by multiple viruses. Here, a comparative transcriptomic analysis was performed to reveal the transcriptional alterations in sweet potato plants infected (VCSP) and non-infected (VFSP) by Sweet potato mild mottle virus (SPFMV), Sweet potato virus Y (SPV2) and Sweet potato virus G (SPVG). A total of 1580 and 12,566 differentially expressed genes (DEGs) were identified in leaves and storage roots of VFSP and VCSP plants, respectively. In leaves, 707 upregulated and 773 downregulated genes were identified, whereas 5653 upregulated and 6913 downregulated genes were identified in storage roots. Gene Ontology (GO) classification and pathway enrichment analysis showed that the expression of genes involved in chloroplast and photosynthesis and brassinosteroid (BR) biosynthesis in leaves and the vitamin biosynthetic process in storage roots was inhibited by co-infection of three viruses: SPFMV, SPV2, and SPVG. This was likely closely related to better photosynthesis and higher contents of Vitamin C (Vc) in storage roots of VFSP than that of VCSP. While some genes involved in ribosome and secondary metabolite-related pathways in leaves and alanine, aspartate, and glutamate metabolism in storage roots displayed higher expression in VCSP than in VFSP. Quantitative real-time PCR analysis demonstrated that the expression patterns of 26 DEGs, including 16 upregulated genes and 10 downregulated genes were consistent with the RNA-seq data from VFSP and VCSP. Taken together, this study integrates the results of morphology, physiology, and comparative transcriptome analyses in leaves and storage roots of VCSP and VFSP to reveal transcriptional alterations in growth- and development-related genes, providing new insight into the molecular mechanisms underlying developmental disorders of sweet potato subjected to co-infection by multiple viruses.
Proteomics Analysis of Tangeretin-Induced Apoptosis through Mitochondrial Dysfunction in Bladder Cancer Cells
Authors: Lin Jen-Jie , Huang Chun-Chieh , Su Yu-Li , Luo Hao-Lun , Lee Nai-Lun , Sung Ming-Tse , Wu Yu-Jen
Published: February 2019

Tangeretin is one of the most abundant compounds in citrus peel, and studies have shown that it possesses anti-oxidant and anti-cancer properties. However, no study has been conducted on bladder cancer cells. Bladder cancer has the second highest mortality rate among urological cancers and is the fifth most common malignancy in the world. Currently, combination chemotherapy is the most common approach by which to treat patients with bladder cancer, and thus identifying more effective chemotherapeutic agents that can be safely administered to patients is a very important research issue. Therefore, this study investigated whether tangeretin can induce apoptosis and identified the signaling pathways of tangeretin-induced apoptosis in human bladder cancer cells using two-dimensional gel electrophoresis (2DGE). The results of the study demonstrated that 60 μM tangeretin reduced the cell survival of a BFTC-905 bladder carcinoma cell line by 42%, and induced early and late apoptosis in the cells. In this study 2DGE proteomics technology identified 41 proteins that were differentially-expressed in tangeretin-treated cells, and subsequently LC–MS/MS analysis was performed to identify the proteins. Based on the functions of the differentially-expressed proteins, the results suggested that tangeretin caused mitochondrial dysfunction and further induced apoptosis in bladder cancer cells. Moreover, western blotting analysis demonstrated that tangeretin treatment disturbed calcium homeostasis in the mitochondria, triggered cytochrome C release, and activated caspase-3 and caspase-9, which led to apoptosis. In conclusion, our results showed that tangeretin-induced apoptosis in human bladder cancer cells is mediated by mitochondrial inactivation, suggesting that tangeretin has the potential to be developed as a new drug for the treatment of bladder cancer.
Novel 1,3,4-Oxadiazole Derivatives Containing a Cinnamic Acid Moiety as Potential Bactericide for Rice Bacterial Diseases
Authors: Wang Shaobo , Gan Xiuhai , Wang Yanju , Li Shaoyuan , Yi Chongfen , Chen Jixiang , He Fangcheng , Yang Yuyuan , Hu Deyu , Song Baoan
Published: February 2019

Rice bacterial leaf blight and leaf streak are two important bacterial diseases of rice, which can result in yield loss. Currently, effective antimicrobials for rice bacterial diseases are still lacking. Thus, to develop highly effective and low-risk bactericides, 31 novel 1,3,4-oxadiazole derivatives containing a cinnamic acid moiety were designed and synthesized. Bioassay results demonstrated that all compounds exhibited good antibacterial activities in vitro. Significantly, compounds 5r and 5t showed excellent antibacterial activities against Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), with the 50% effective concentration (EC50) values of 0.58 and 0.34, and 0.44 and 0.20 μg/mL, respectively. These compounds were much better than thiodiazole copper (123.10 and 161.52 μg/mL) and bismerthiazol (85.66 and 110.96 μg/mL). Moreover, compound 5t had better protective and curative activities against rice bacterial leaf blight and leaf streak than thiodiazole copper and bismerthiazol in vivo. Simultaneously, the in vivo efficacy of the compounds was demonstrated by real-time quantitative PCR to quantify bacterial titers. In addition, a three-dimensional quantitative structure–activity relationship model was created and presented good predictive ability. This work provides support for 1,3,4-oxadiazole derivatives containing a cinnamic acid moiety as a potential new bactericide for rice bacterial diseases.
Impact of Long Non-Coding RNA HOTAIR Genetic Variants on the Susceptibility and Clinicopathologic Characteristics of Patients with Urothelial Cell Carcinoma
Authors: Tung Min-Che , Wen Yu-Ching , Wang Shian-Shiang , Lin Yung-Wei , Chow Jyh-Ming , Yang Shun-Fa , Chien Ming-Hsien
Published: February 2019

Increasing evidence shows that dysregulated expression of long non-coding (lnc)RNAs can serve as diagnostic or prognostic markers in urothelial cell carcinoma (UCC), the most common pathological type of bladder cancer. lncRNA HOX transcript antisense RNA (HOTAIR) was shown to promote tumor progression and be associated with a poor prognosis in multiple cancers including bladder cancer. Polymorphisms of HOTAIR were recently linked to a predisposition for diverse malignancies. Herein we conducted a case-control study to evaluate whether genetic polymorphisms of HOTAIR were associated with UCC risk and clinicopathologic characteristics. Four loci (rs920778 T>C, rs1899663 G>T, rs4759314 A>G, and rs12427129, C>T) of HOTAIR were genotyped by a TaqMan allelic discrimination method in 431 cases and 862 controls. We found that female patients who carried AG + GG genotype of rs4759314 were associated with an increased UCC risk after controlling for age and tobacco consumption (adjusted odds ratio (AOR) = 1.92, 95% confidence interval (CI): 1.01–3.64, p = 0.047) and a lower overall survival rate (p = 0.008). Moreover, patients with a smoking habit or younger age (≤65 years), who had at least one T allele of HOTAIR rs12427129 were at a higher risk of developing advance tumor T satge (p = 0.046), compared to those patients with CC homozygotes. In contrast, rs920778 C allele carriers were negatively correlated with the development of lymph node metastasis (OR = 0.51, 95% CI: 0.28–0.94, p = 0.031). Further analyses of clinical datasets revealed correlations of the expression of HOTAIR with tumor metastasis and a poor survival rate in patients with UCC. Our results verified the diverse impacts of HOTAIR variants on UCC susceptibility and clinicopathologic characteristics.
TNFAIP3, TNIP1, and MyD88 Polymorphisms Predict Septic-Shock-Related Death in Patients Who Underwent Major Surgery
Authors: Jiménez-Sousa Maria Ángeles , Fadrique Alejandra , Liu Pilar , Fernández-Rodríguez Amanda , Lorenzo-López Mario , Gómez-Sánchez Esther , Gómez-Sanz Alicia , Heredia-Rodríguez María , Gómez-Pesquera Estefanía , Martínez Isidoro , Tamayo Eduardo , Resino Salvador
Published: February 2019

Background: In many immune-related diseases, inflammatory responses and several clinical outcomes are related to increased NF-κB activity. We aimed to evaluate whether SNPs related to the NF-κB signaling pathway are associated with higher susceptibility to infection, septic shock, and septic-shock-related death in European patients who underwent major surgery. Methods: We performed a case-control study on 184 patients with septic shock and 212 with systemic inflammatory response syndrome, and a longitudinal substudy on septic shock patients. Thirty-three SNPs within genes belonging to or regulating the NF-κB signaling pathway were genotyped by Agena Bioscience’s MassARRAY platform. Results: No significant results were found for susceptibility to infection and septic shock in the multivariate analysis after adjusting for multiple comparisons. Regarding septic-shock-related death, patients with TNFAIP3 rs6920220 AA, TNIP1 rs73272842 AA, TNIP1 rs3792783 GG, and TNIP1 rs7708392 CC genotypes had the highest risk of septic-shock-related death in the first 28 and 90 days. Also, the MyD88 rs7744 GG genotype was associated with a higher risk of death during the first 90 days. Haplotype analysis shows us that patients with the TNIP1 GAG haplotype (composed of rs73272842, rs3792783, and rs7708392) had a lower risk of death in the first 28 days and the TNIP1 AGC haplotype was associated with a higher risk of death in the first 90 days. Conclusions: The SNPs in the genes TNFAIP3, TNIP1, and MyD88 were linked to the risk of septic-shock-related death in patients who underwent major surgery.
Analytical Performance of the Threshold Voltage and Subthreshold Swing of CSDG MOSFET
Authors: Maduagwu Uchechukwu A. , Srivastava Viranjay M.
Published: February 2019

In this research work, the threshold voltage and subthreshold swing of cylindrical surrounding double-gate (CSDG) MOSFET have been analyzed. These analyses are based on the analytical solution of 2D Poisson equation using evanescent-mode analysis (EMA). This EMA provides the better approach in solving the 2D Poisson equation by considering the oxide and Silicon regions as a two-dimensional problem, to produce physically consistent results with device simulation for better device performance. Unlike other models such as polynomial exponential and parabolic potential approximation (PPA) which consider the oxide and silicon as one-dimensional problem. Using the EMA, the 2D Poisson equation is decoupled into 1D Poisson equation which represent the long channel potential and 2D Laplace equation describing the impacts of short channel effects (SCEs) in the channel potential. Furthermore, the derived channel potential close-form expression is extended to determine the threshold voltage and subthreshold behavior of the proposed CSDG MOSFET device. This model has been evaluated with various device parameters such as radii Silicon film thickness, gate oxide thickness, and the channel length to analyze the behavior of the short channel effects in the proposed CSDG MOSFET. The accuracy of the derived expressions have been validated with the mathematical and numerical simulation.
Optimization of Maleinized Linseed Oil Loading as a Biobased Compatibilizer in Poly(Butylene Succinate) Composites with Almond Shell Flour
Authors: Liminana Patricia , Garcia-Sanoguera David , Quiles-Carrillo Luis , Balart Rafael , Montanes Nestor
Published: February 2019

Green composites of poly(butylene succinate) (PBS) were manufactured with almond shell flour (ASF) by reactive compatibilization with maleinized linseed oil *MLO) by extrusion and subsequent injection molding. ASF was kept constant at 30 wt %, while the effect of different MLO loading on mechanical, thermal, thermomechanical, and morphology properties was studied. Uncompatibilized PBS/ASF composites show a remarkable decrease in mechanical properties due to the nonexistent polymer‒filler interaction, as evidenced by field emission scanning electron microscopy (FESEM). MLO provides a plasticization effect on PBS/ASF composites but, in addition, acts as a compatibilizer agent since the maleic anhydride groups contained in MLO are likely to react with hydroxyl groups in both PBS end chains and ASF particles. This compatibilizing effect is observed by FESEM with a reduction of the gap between the filler particles and the surrounding PBS matrix. In addition, the Tg of PBS increases from −28 °C to −12 °C with an MLO content of 10 wt %, thus indicating compatibilization. MLO has been validated as an environmentally friendly additive to PBS/ASF composites to give materials with high environmental efficiency.
Investigating the Durability of Iodine Waste Forms in Dilute Conditions
Authors: Asmussen R. Matthew , Ryan Joseph V. , Matyas Josef , Crum Jarrod V. , Reiser Joelle T. , Avalos Nancy , McElroy Erin M. , Lawter Amanda R. , Canfield Nathan C.
Published: February 2019

To prevent the release of radioiodine during the reprocessing of used nuclear fuel or in the management of other wastes, many technologies have been developed for iodine capture. The capture is only part of the challenge as a durable waste form is required to ensure safe disposal of the radioiodine. This work presents the first durability studies in dilute conditions of two AgI-containing waste forms: hot-isostatically pressed silver mordenite (AgZ) and spark plasma sintered silver-functionalized silica aerogel (SFA) iodine waste forms (IWF). Using the single-pass flow-through (SPFT) test method, the dissolution rates respective to Si, Al, Ag and I were measured for variants of the IWFs. By combining solution and solid analysis information on the corrosion mechanism neutral-to-alkaline conditions was elucidated. The AgZ samples were observed to have corrosion preferentially occur at secondary phases with higher Al and alkali content. These phases contained a lower proportion of I compared with the matrix. The SFA samples experienced a higher extent of corrosion at Si-rich particles, but an increased addition of Si to the waste led to an improvement in corrosion resistance. The dissolution rates for the IWF types are of similar magnitude to other Si-based waste form materials measured using SPFT.
Pore Architectures and Mechanical Properties of Porous α-SiAlON Ceramics Fabricated via Unidirectional Freeze Casting Based on Camphene-Templating
Authors: Hou Zhaoping , Ye Feng , Liu Qiang , Liu Limeng , Jiang Haiwei , Zhang Shaowei
Published: February 2019

Porous α-SiAlON ceramics were fabricated using the camphene-based unidirectional freeze casting method, in which a gradient porous structure was formed as a result of the decreased solidification velocity in the freezing direction. Microstructure, porosity and pore size distribution of different parts of as-prepared samples were examined and compared, and correlated with their mechanical properties. For a given solid loading, the overall pore size and porosity of the top part were greater than those of the bottom part. Interestingly, despite its higher porosity, the flexural strength and fracture toughness of the top part were both higher than those of the bottom part, suggesting that apart from porosity, pore morphology and size affected mechanical properties of as-prepared porous α-SiAlON ceramics.
Smart Control of Nitroxide-Mediated Polymerization Initiators’ Reactivity by pH, Complexation with Metals, and Chemical Transformations
Authors: Edeleva Mariya , Audran Gerard , Marque Sylvain , Bagryanskaya Elena
Published: February 2019

Because alkoxyamines are employed in a number of important applications, such as nitroxide-mediated polymerization, radical chemistry, redox chemistry, and catalysis, research into their reactivity is especially important. Typically, the rate of alkoxyamine homolysis is strongly dependent on temperature. Nonetheless, thermal regulation of such reactions is not always optimal. This review describes various ways to reversibly change the rate of C–ON bond homolysis of alkoxyamines at constant temperature. The major methods influencing C–ON bond homolysis without alteration of temperature are protonation of functional groups in an alkoxyamine, formation of metal–alkoxyamine complexes, and chemical transformation of alkoxyamines. Depending on the structure of an alkoxyamine, these approaches can have a significant effect on the homolysis rate constant, by a factor of up to 30, and can shorten the half-lifetime from days to seconds. These methods open new prospects for the application of alkoxyamines in biology and increase the safety of (and control over) the nitroxide-mediated polymerization method.