bluuebunny/crossref_metadata_2025 huggingface dataset:
Human Amnion-Derived Mesenchymal Stromal Cells: A New Potential Treatment for Carbapenem-Resistant Enterobacterales in Decompensated Cirrhosis
Authors: Pampalone Mariangela , Vitale Giampiero , Gruttadauria Salvatore , Amico Giandomenico , Iannolo Gioacchin , Douradinha Bruno , Mularoni Alessandra , Conaldi Pier Giulio , Pietrosi Giada
Published: January 2022

Background: Spontaneous bacterial peritonitis (SBP) is a severe and often fatal infection in patients with decompensated cirrhosis and ascites. The only cure for SBP is antibiotic therapy, but the emerging problem of bacterial resistance requires novel therapeutic strategies. Human amniotic mesenchymal stromal cells (hA-MSCs) possess immunomodulatory and anti-inflammatory properties that can be harnessed as a therapy in such a context. Methods: An in vitro applications of hA-MSCs in ascitic fluid (AF) of cirrhotic patients, subsequently infected with carbapenem-resistant Enterobacterales, was performed. We evaluated the effects of hA-MSCs on bacterial load, innate immunity factors, and macrophage phenotypic expression. Results: hA-MSCs added to AF significantly reduce the proliferation of both bacterial strains at 24 h and diversely affect M1 and M2 polarization, C3a complement protein, and ficolin 3 concentrations during the course of infection, in a bacterial strain-dependent fashion. Conclusion: This study shows the potential usefulness of hA-MSC in treating ascites infected with carbapenem-resistant bacteria and lays the foundation to further investigate antibacterial and anti-inflammatory roles of hA-MSC in in vivo models.
COE2 Is Required for the Root Foraging Response to Nitrogen Limitation
Authors: Wu Rui , Liu Zhixin , Wang Jiajing , Guo Chenxi , Zhou Yaping , Bawa George , Rochaix Jean-David , Sun Xuwu
Published: January 2022

There are numerous exchanges of signals and materials between leaves and roots, including nitrogen, which is one of the essential nutrients for plant growth and development. In this study we identified and characterized the Chlorophyll A/B-Binding Protein (CAB) (named coe2 for CAB overexpression 2) mutant, which is defective in the development of chloroplasts and roots under normal growth conditions. The phenotype of coe2 is caused by a mutation in the Nitric Oxide Associated (NOA1) gene that is implicated in a wide range of chloroplast functions including the regulation of metabolism and signaling of nitric oxide (NO). A transcriptome analysis reveals that expression of genes involved in metabolism and lateral root development are strongly altered in coe2 seedlings compared with WT. COE2 is expressed in hypocotyls, roots, root hairs, and root caps. Both the accumulation of NO and the growth of lateral roots are enhanced in WT but not in coe2 under nitrogen limitation. These new findings suggest that COE2-dependent signaling not only coordinates gene expression but also promotes chloroplast development and function by modulating root development and absorption of nitrogen compounds.
Artificial Intelligence and Democratization of the Use of Lung Ultrasound in COVID-19: On the Feasibility of Automatic Calculation of Lung Ultrasound Score
Authors: Camacho Jorge , Muñoz Mario , Genovés Vicente , Herraiz Joaquín L. , Ortega Ignacio , Belarra Adrián , González Ricardo , Sánchez David , Giacchetta Roberto Carlos , Trueba-Vicente Ángela , Tung-Chen Yale
Published: January 2022

During the COVID-19 pandemic, lung ultrasound has been revealed as a powerful technique for diagnosis and follow-up of pneumonia, the principal complication of SARS-CoV-2 infection. Nevertheless, being a relatively new and unknown technique, the lack of trained personnel has limited its application worldwide. Computer-aided diagnosis could possibly help to reduce the learning curve for less experienced physicians, and to extend such a new technique such as lung ultrasound more quickly. This work presents the preliminary results of the ULTRACOV (Ultrasound in Coronavirus disease) study, aimed to explore the feasibility of a real-time image processing algorithm for automatic calculation of the lung ultrasound score (LUS). A total of 28 patients positive on COVID-19 were recruited and scanned in 12 thorax zones following the lung score protocol, saving a 3 s video at each probe position. Those videos were evaluated by an experienced physician and by a custom developed automated detection algorithm, looking for A-Lines, B-Lines, consolidations, and pleural effusions. The agreement between the findings of the expert and the algorithm was 88.0% for B-Lines, 93.4% for consolidations and 99.7% for pleural effusion detection, and 72.8% for the individual video score. The standard deviation of the patient lung score difference between the expert and the algorithm was ±2.2 points over 36. The exam average time with the ULTRACOV prototype was 5.3 min, while with a conventional scanner was 12.6 min. Conclusion: A good agreement between the algorithm output and an experienced physician was observed, which is a first step on the feasibility of developing a real-time aided-diagnosis lung ultrasound equipment. Additionally, the examination time was reduced to less than half with regard to a conventional ultrasound exam. Acquiring a complete lung ultrasound exam within a few minutes is possible using fairly simple ultrasound machines that are enhanced with artificial intelligence, such as the one we propose. This step is critical to democratize the use of lung ultrasound in these difficult times.
Cognitive Digital Twins for Resilience in Production: A Conceptual Framework
Authors: Eirinakis Pavlos , Lounis Stavros , Plitsos Stathis , Arampatzis George , Kalaboukas Kostas , Kenda Klemen , Lu Jinzhi , Rožanec Jože M. , Stojanovic Nenad
Published: January 2022

Digital Twins (DTs) are a core enabler of Industry 4.0 in manufacturing. Cognitive Digital Twins (CDTs), as an evolution, utilize services and tools towards enabling human-like cognitive capabilities in DTs. This paper proposes a conceptual framework for implementing CDTs to support resilience in production, i.e., to enable manufacturing systems to identify and handle anomalies and disruptive events in production processes and to support decisions to alleviate their consequences. Through analyzing five real-life production cases in different industries, similarities and differences in their corresponding needs are identified. Moreover, a connection between resilience and cognition is established. Further, a conceptual architecture is proposed that maps the tools materializing cognition within the DT core together with a cognitive process that enables resilience in production by utilizing CDTs.
The Role of Trustworthiness Facets for Developing Social Media Applications: A Structured Literature Review
Authors: Borchert Angela , Heisel Maritta
Published: January 2022

This work reviews existing research about attributes, which are assessed by individuals to evaluate the trustworthiness of (i) software applications, (ii) organizations (e.g., service providers), and (iii) other individuals. As these parties are part of social media services, previous research has identified the need for users to assess their trustworthiness. Based on the trustworthiness assessment, users decide whether they want to interact with them and whether such interactions appear safe. The literature review encompasses 264 works from which so-called trustworthiness facets of 100 papers could be identified. In addition to an overview of trustworthiness facets, this work further introduces a guideline for software engineers on how to select appropriate trustworthiness facets during the analysis of the problem space for the development of specific social media applications. It is exemplified by the problem of “catfishing” in online dating.
Dual-Hybrid Modeling for Option Pricing of CSI 300ETF
Authors: Zhao Kejing , Zhang Jinliang , Liu Qing
Published: January 2022

The reasonable pricing of options can effectively help investors avoid risks and obtain benefits, which plays a very important role in the stability of the financial market. The traditional single option pricing model often fails to meet the ideal expectations due to its limited conditions. Combining an economic model with a deep learning model to establish a hybrid model provides a new method to improve the prediction accuracy of the pricing model. This includes the usage of real historical data of about 10,000 sets of CSI 300 ETF options from January to December 2020 for experimental analysis. Aiming at the prediction problem of CSI 300ETF option pricing, based on the importance of random forest features, the Convolutional Neural Network and Long Short-Term Memory model (CNN-LSTM) in deep learning is combined with a typical stochastic volatility Heston model and stochastic interests CIR model in parameter models. The dual hybrid pricing model of the call option and the put option of CSI 300ETF is established. The dual-hybrid model and the reference model are integrated with ridge regression to further improve the forecasting effect. The results show that the dual-hybrid pricing model proposed in this paper has high accuracy, and the prediction accuracy is tens to hundreds of times higher than the reference model; moreover, MSE can be as low as 0.0003. The article provides an alternative method for the pricing of financial derivatives.
3D Reconstruction with Coronary Artery Based on Curve Descriptor and Projection Geometry-Constrained Vasculature Matching
Authors: Tong Jijun , Xu Shuai , Wang Fangliang , Qi Pengjia
Published: January 2022

This paper presents a novel method based on a curve descriptor and projection geometry constrained for vessel matching. First, an LM (Leveberg–Marquardt) algorithm is proposed to optimize the matrix of geometric transformation. Combining with parameter adjusting and the trust region method, the error between 3D reconstructed vessel projection and the actual vessel can be minimized. Then, CBOCD (curvature and brightness order curve descriptor) is proposed to indicate the degree of the self-occlusion of blood vessels during angiography. Next, the error matrix constructed from the error of epipolar matching is used in point pairs matching of the vascular through dynamic programming. Finally, the recorded radius of vessels helps to construct ellipse cross-sections and samples on it to get a point set around the centerline and the point set is converted to mesh for reconstructing the surface of vessels. The validity and applicability of the proposed methods have been verified through experiments that result in the significant improvement of 3D reconstruction accuracy in terms of average back-projection errors. Simultaneously, due to precise point-pair matching, the smoothness of the reconstructed 3D coronary artery is guaranteed.
Reversing Jensen’s Inequality for Information-Theoretic Analyses
Authors: Merhav Neri
Published: January 2022

In this work, we propose both an improvement and extensions of a reverse Jensen inequality due to Wunder et al. (2021). The new proposed inequalities are fairly tight and reasonably easy to use in a wide variety of situations, as demonstrated in several application examples that are relevant to information theory. Moreover, the main ideas behind the derivations turn out to be applicable to generate bounds to expectations of multivariate convex/concave functions, as well as functions that are not necessarily convex or concave.
Nitrogen Acquisition Strategies Mediated by Insect Symbionts: A Review of Their Mechanisms, Methodologies, and Case Studies
Authors: Ren Xueming , Guo Ruxin , Akami Mazarin , Niu Changying
Published: January 2022

Nitrogen is usually a restrictive nutrient that affects the growth and development of insects, especially of those living in low nitrogen nutrient niches. In response to the low nitrogen stress, insects have gradually developed symbiont-based stress response strategies—biological nitrogen fixation and nitrogenous waste recycling—to optimize dietary nitrogen intake. Based on the above two patterns, atmospheric nitrogen or nitrogenous waste (e.g., uric acid, urea) is converted into ammonia, which in turn is incorporated into the organism via the glutamine synthetase and glutamate synthase pathways. This review summarized the reaction mechanisms, conventional research methods and the various applications of biological nitrogen fixation and nitrogenous waste recycling strategies. Further, we compared the bio-reaction characteristics and conditions of two strategies, then proposed a model for nitrogen provisioning based on different strategies.
Detection of Yeast-like Symbionts in Brown Planthopper Reared on Different Resistant Rice Varieties Combining DGGE and Absolute Quantitative Real-Time PCR
Authors: Lai Chengling , Hou Yun , Hao Peiying , Pang Kun , Yu Xiaoping
Published: January 2022

The brown planthopper (BPH), Nilaparvata lugens, is a serious pest of rice throughout Asia. Yeast-like symbionts (YLS) are endosymbionts closely linked with the development of BPH and the adapted mechanism of BPH virulence to resistant plants. In this study, we used semi-quantitative DGGE and absolute quantitative real-time PCR (qPCR) to quantify the number of the three YLS strains (Ascomycetes symbionts, Pichia-like symbionts, and Candida-like symbionts) that typically infect BPH in the nymphal stages and in newly emerged female adults. The quantities of each of the three YLS assessed increased in tandem with the developing nymphal instar stages, peaking at the fourth instar stage, and then declined significantly at the fifth instar stage. However, the amount of YLS present recovered sharply within the emerging adult females. Additionally, we estimated the quantities of YLS for up to eight generations after their inoculation onto resistant cultivars (Mudgo, ASD7, and RH) to reassociate the dynamics of YLS with the fitness of BPH. The minimum number of each YLS was detected in the second generation and gradually increased from the third generation with regard to resistant rice varieties. In addition, the Ascomycetes symbionts of YLS were found to be the most abundant of the three YLS strains tested for all of the development stages of BPH.
Lifelong Disadvantage and Late Adulthood Frailty
Authors: Zanasi Francesca , De Santis Gustavo , Pirani Elena
Published: January 2022

Frailty is a complex state of objective and subjective vulnerability. It tends to increase with age, but the process is influenced by previous life course, especially previous disadvantages. The aim of this paper is to examine how the disadvantages suffered in adulthood (25 to 59 years) in four domains (unemployment, financial hardship, stress, and bad health) affect frailty in late adulthood (60 to 79 years). Using linear regression models on data from the Survey of Health, Ageing, and Retirement in Europe (2004–2017), we estimate frailty levels for several age groups (60–64, 65–69, 70–74, 75–79) accounting for both the persistence of these disadvantages over time and their coexistence, i.e., the number of years when they were simultaneously experienced. Results show that while frailty increases with age, as expected, there is also evidence of an accumulation of risks: the longer the periods of adult life affected by unemployment, stress, financial hardship or, most importantly, bad health, the frailer individuals are in their late years. Furthermore, periods of coexisting disadvantages in adulthood translate into additional frailty in late life. Our findings highlight the importance of fighting disadvantages early in life: long-term improvements in terms of reduced frailty (a concept interrelated with health) may be substantial.
Endomyocardial Biopsy in Pediatric Myocarditis and Dilated Cardiomyopathy: A Tool in Search for a Role
Authors: Pilati Mara , Rebonato Micol , Formigari Roberto , Butera Gianfranco
Published: January 2022

Endomyocardial biopsy (EMB) is a well-known diagnostic tool for the investigation and treatment of myocardial diseases and remains the gold standard for the diagnosis of myocarditis. Due to its invasiveness, with a complication rate ranging from 1 to 15%, its role in the diagnostic work-up of pediatric heart failure is not well established. The aim of this review is to define the role of EMB as diagnostic technique in the work up of children presenting with severe left ventricular dysfunction with the support of our center experience.
Dosimetric Effects of Air Cavities for MRI-Guided Online Adaptive Radiation Therapy (MRgART) of Prostate Bed after Radical Prostatectomy
Authors: Pham Jonathan , Cao Minsong , Yoon Stephanie M. , Gao Yu , Kishan Amar U. , Yang Yingli
Published: January 2022

Purpose: To evaluate dosimetric impact of air cavities and their corresponding electron density correction for 0.35 tesla (T) Magnetic Resonance-guided Online Adaptive Radiation Therapy (MRgART) of prostate bed patients. Methods: Three 0.35 T MRgRT plans (anterior–posterior (AP) beam, AP–PA beams, and clinical intensity modulated radiation therapy (IMRT)) were generated on a prostate bed patient’s (Patient A) planning computed tomography (CT) with artificial rectal air cavities of various sizes (0–3 cm, 0.5 cm increments). Furthermore, two 0.35 T MRgART plans (‘Deformed’ and ‘Override’) were generated on a prostate bed patient’s (Patient B) daily magnetic resonance image (MRI) with artificial rectal air cavities of various sizes (0–3 cm, 0.5 cm increments) and on five prostate bed patient’s (Patient 1–5) daily MRIs (2 MRIs: Fraction A and B) with real air cavities. For each MRgART plan, daily MRI electron density map was obtained by deformable registration with simulation CT. In the ‘Deformed’ plan, a clinical IMRT plan is calculated on the daily MRI with electron density map obtained from deformable registration only. In the ‘Override’ plan, daily MRI and simulation CT air cavities are manually corrected and bulk assigned air and water density on the registered electron density map, respectively. Afterwards, the clinical IMRT plan is calculated. Results: For the MRgRT plans, AP and AP–PA plans’ rectum/rectal wall max dose increased with increasing air cavity size, where the 3 cm air cavity resulted in a 20%/17% and 13%/13% increase, relative to no air cavity, respectively. Clinical IMRT plan was robust to air cavity size, where dose change remained less than 1%. For the MRgART plans, daily MRI electron density maps, obtained from deformable registration with simulation CT, was unable to accurately produce electron densities reflecting the air cavities. However, for the artificial daily MRI air cavities, dosimetric change between ‘Deformed’ and ‘Override’ plan was small (<4%). Similarly, for the real daily MRI air cavities, clinical constraint changes between ‘Deformed’ and ‘Override’ plan was negligible and did not lead to change in clinical decision for adaptive planning except for two fractions. In these fractions, the ‘Override’ plan indicated that the bladder max dose and rectum V35.7 exceeded the constraint, while the ‘Deformed’ plan showed acceptable dose, although the absolute difference was only 0.3 Gy and 0.03 cc, respectively. Conclusion: Clinical 0.35 T IMRT prostate bed plans are dosimetrically robust to air cavities. MRgART air cavity electron density correction shows clinically insignificant change and is not warranted on low-field systems.
PECAM1, COL4A2, PHACTR1, and LMOD1 Gene Polymorphisms in Patients with Unstable Angina
Authors: Kosiński Krzysztof , Malinowski Damian , Safranow Krzysztof , Dziedziejko Violetta , Pawlik Andrzej
Published: January 2022

Coronary artery disease (CAD) is a syndrome resulting from myocardial ischaemia of heterogeneous pathomechanism. Environmental and genetic factors contribute to its development. Atherosclerotic plaques that significantly narrow the lumen of coronary arteries cause symptoms of myocardial ischaemia. Acute coronary incidents are most often associated with plaque rupture or erosion accompanied by local activation of the coagulation system with thrombus formation. Plaque formation and stability are influenced by endothelial function and vascular smooth muscle cell function. In this study, we investigated the association between polymorphisms in genes affecting endothelial and vascular smooth muscle cell (VSMC) function and the occurrence of unstable angina pectoris. The aim of this study was to evaluate the association between the PECAM1 (rs1867624), COL4A2 (rs4773144), PHACTR1 (rs9349379) and LMOD1 (rs2820315) gene polymorphisms and the risk of unstable angina. The study included 232 patients with unstable angina diagnosed on the basis of clinical symptoms and coronary angiography and 144 healthy subjects with no significant coronary lumen stenosis at coronary angiography. There were no statistically significant differences in the distribution of COL4A2 rs4773144 and PECAM1 rs1867624 gene polymorphisms between patients with unstable angina and control subjects. In patients with unstable angina, there was an increased frequency of PHACTR1 rs9349379 G allele carriers (GG and AG genotypes) (GG+AG vs. AA, OR 1.71; 95% CI 1.10–2.66, p = 0.017) and carriers of the LMOD1 rs2820315 T allele (TT and CT genotypes) (TT+CT vs. CC, OR 1.65; 95% CI 1.09–2.51, p = 0.019) compared to the control group. The association between these alleles and unstable angina was confirmed by multivariate logistic regression analysis, in which the number of G (PHACTR1 rs9349379) and T (LMOD1 rs2820315) alleles was an independent risk factor for unstable angina. The results suggest an association between PHACTR1 rs9349379 and LMOD1 rs2820315 polymorphisms and the risk of unstable angina.
Therapeutic Advances in Diabetic Nephropathy
Authors: Sawaf Hanny , Thomas George , Taliercio Jonathan J. , Nakhoul Georges , Vachharajani Tushar J. , Mehdi Ali
Published: January 2022

Diabetic kidney disease (DKD) is the most common cause of end-stage kidney disease (ESKD) in the United States. Risk factor modification, such as tight control of blood glucose, management of hypertension and hyperlipidemia, and the use of renin–angiotensin–aldosterone system (RAAS) blockade have been proven to help delay the progression of DKD. In recent years, new therapeutics including sodium-glucose transport protein 2 (SGLT2) inhibitors, endothelin antagonists, glucagon like peptide-1 (GLP-1) agonists, and mineralocorticoid receptor antagonists (MRA), have provided additional treatment options for patients with DKD. This review discusses the various treatment options available to treat patients with diabetic kidney disease.
Proactive Management of Intraoperative Hypotension Reduces Biomarkers of Organ Injury and Oxidative Stress during Elective Non-Cardiac Surgery: A Pilot Randomized Controlled Trial
Authors: Murabito Paolo , Astuto Marinella , Sanfilippo Filippo , La Via Luigi , Vasile Francesco , Basile Francesco , Cappellani Alessandro , Longhitano Lucia , Distefano Alfio , Li Volti Giovanni
Published: January 2022

Background: Intraoperative hypotension is associated with increased postoperative morbidity and mortality. Methods: We randomly assigned patients undergoing major general surgery to early warning system (EWS) and hemodynamic algorithm (intervention group, n = 20) or standard care (n = 20). The primary outcome was the difference in hypotension (defined as mean arterial pressure < 65 mmHg) and as secondary outcome surrogate markers of organ injury and oxidative stress. Results: The median number of hypotensive episodes was lower in the intervention group (−5.0 (95% CI: −9.0, −0.5); p < 0.001), with lower time spent in hypotension (−12.8 min (95% CI: −38.0, −2.3 min); p = 0.048), correspondent to −4.8% of total surgery time (95% CI: −12.7, 0.01%; p = 0.048).The median time-weighted average of hypotension was 0.12 mmHg (0.35) in the intervention group and 0.37 mmHg (1.11) in the control group, with a median difference of −0.25 mmHg (95% CI: −0.85, −0.01; p = 0.025). Neutrophil Gelatinase-Associated Lipocalin (NGAL) correlated with time-weighted average of hypotension (R = 0.32; p = 0.038) and S100B with number of hypotensive episodes, absolute time of hypotension, relative time of hypotension and time-weighted average of hypotension (p < 0.001 for all). The intervention group showed lower Neuronal Specific Enolase (NSE) and higher reduced glutathione when compared to the control group. Conclusions: The use of an EWS coupled with a hemodynamic algorithm resulted in reduced intraoperative hypotension, reduced NSE and oxidative stress.
Homocysteine Plasmatic Concentration in Brain-Injured Neurocritical Care Patients: Systematic Review of Clinical Evidence
Authors: Lauretta Maria Paola , Melotti Rita Maria , Sangermano Corinne , George Anneliya Maria , Badenes Rafael , Bilotta Federico
Published: January 2022

Background: Hyperhomocysteinemia (HHcy) is considered as an independent risk factor for several diseases, such as cardiovascular, neurological and autoimmune conditions. Atherothrombotic events, as a result of endothelial dysfunction and increased inflammation, are the main mechanisms involved in vascular damage. This review article reports clinical evidence on the relationship between the concentration of plasmatic homocysteine (Hcy) and acute brain injury (ABI) in neurocritical care patients. Materials and methods: a systematic search of articles in the PubMed and EMBASE databases was conducted, of which only complete studies, published in English in peer-reviewed journals, were included. Results: A total of 33 articles, which can be divided into the following 3 subchapters, are present: homocysteine and acute ischemic stroke (AIS); homocysteine and traumatic brain injury (TBI); homocysteine and intracranial hemorrhage (ICH)/subarachnoid hemorrhage (SAH). This confirms that HHcy is an independent risk factor for ABI and a marker of poor prognosis in the case of stroke, ICH, SAH and TBI. Conclusions: Several studies elucidate that Hcy levels influence the patient’s prognosis in ABI and, in some cases, the risk of recurrence. Hcy appears as biochemical marker that can be used by neuro-intensivists as an indicator for risk stratification. Moreover, a nutraceutical approach, including folic acid, the vitamins B6 and B12, reduces the risk of thrombosis, cardiovascular and neurological dysfunction in patients with severe HHcy that were admitted for neurocritical care.
3D Printing of Hierarchically Porous Lattice Structures Based on Åkermanite Glass Microspheres and Reactive Silicone Binder
Authors: Dasan Arish , Kraxner Jozef , Grigolato Luca , Savio Gianpaolo , Elsayed Hamada , Galusek Dušan , Bernardo Enrico
Published: January 2022

The present study illustrates the manufacturing method of hierarchically porous 3D scaffolds based on åkermanite as a promising bioceramic for stereolithography. The macroporosity was designed by implementing 3D models corresponding to different lattice structures (cubic, diamond, Kelvin, and Kagome). To obtain micro-scale porosity, flame synthesized glass microbeads with 10 wt% of silicone resins were utilized to fabricate green scaffolds, later converted into targeted bioceramic phase by firing at 1100 °C in air. No chemical reaction between the glass microspheres, crystallizing into åkermanite, and silica deriving from silicone oxidation was observed upon heat treatment. Silica acted as a binder between the adjacent microspheres, enhancing the creation of microporosity, as documented by XRD, and SEM coupled with EDX analysis. The formation of ‘spongy’ struts was confirmed by infiltration with Rhodamine B solution. The compressive strength of the sintered porous scaffolds was up to 0.7 MPa with the porosity of 68–84%.
Late Quaternary Marine Terraces and Tectonic Uplift Rates of the Broader Neapolis Area (SE Peloponnese, Greece)
Authors: Karymbalis Efthimios , Tsanakas Konstantinos , Tsodoulos Ioannis , Gaki-Papanastassiou Kalliopi , Papanastassiou Dimitrios , Batzakis Dimitrios-Vasileios , Stamoulis Konstantinos
Published: January 2022

Marine terraces are geomorphic markers largely used to estimate past sea-level positions and surface deformation rates in studies focused on climate and tectonic processes worldwide. This paper aims to investigate the role of tectonic processes in the late Quaternary evolution of the coastal landscape of the broader Neapolis area by assessing long-term vertical deformation rates. To document and estimate coastal uplift, marine terraces are used in conjunction with Optically Stimulated Luminescence (OSL) dating and correlation to late Quaternary eustatic sea-level variations. The study area is located in SE Peloponnese in a tectonically active region. Geodynamic processes in the area are related to the active subduction of the African lithosphere beneath the Eurasian plate. A series of 10 well preserved uplifted marine terraces with inner edges ranging in elevation from 8 ± 2 m to 192 ± 2 m above m.s.l. have been documented, indicating a significant coastal uplift of the study area. Marine terraces have been identified and mapped using topographic maps (at a scale of 1:5000), aerial photographs, and a 2 m resolution Digital Elevation Model (DEM), supported by extensive field observations. OSL dating of selected samples from two of the terraces allowed us to correlate them with late Pleistocene Marine Isotope Stage (MIS) sea-level highstands and to estimate the long-term uplift rate. Based on the findings of the above approach, a long-term uplift rate of 0.36 ± 0.11 mm a−1 over the last 401 ± 10 ka has been suggested for the study area. The spatially uniform uplift of the broader Neapolis area is driven by the active subduction of the African lithosphere beneath the Eurasian plate since the study area is situated very close (~90 km) to the active margin of the Hellenic subduction zone.
Dietary Agaricus blazei Spent Substrate Improves Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae In Vivo
Authors: Lee Po-Tsang , Wu Yu-Sheng , Tseng Chung-Chih , Lu Jia-Yu , Lee Meng-Chou
Published: January 2022

This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.
Clinical Management of Chronic Pelvic Pain in Endometriosis Unresponsive to Conventional Therapy
Authors: Pereira Augusto , Herrero-Trujillano Manuel , Vaquero Gema , Fuentes Lucia , Gonzalez Sofia , Mendiola Agustin , Perez-Medina Tirso
Published: January 2022

Background: Although several treatments are currently available for chronic pelvic pain, 30–60% of patients do not respond to them. Therefore, these therapeutic options require a better understanding of the mechanisms underlying endometriosis-induced pain. This study focuses on pain management after failure of conventional therapy. Methods: We reviewed clinical data from 46 patients with endometriosis and chronic pelvic pain unresponsive to conventional therapies at Puerta de Hierro University Hospital Madrid, Spain from 2018 to 2021. Demographic data, clinical and exploratory findings, treatment received, and outcomes were collected. Results: Median age was 41.5 years, and median pain intensity was VAS: 7.8/10. Nociceptive pain and neuropathic pain were identified in 98% and 70% of patients, respectively. The most common symptom was abdominal pain (78.2%) followed by pain with sexual intercourse (65.2%), rectal pain (52.1%), and urologic pain (36.9%). A total of 43% of patients responded to treatment with neuromodulators. Combined therapies for myofascial pain syndrome, as well as treatment of visceral pain with inferior or superior hypogastric plexus blocks, proved to be very beneficial. S3 pulsed radiofrequency (PRF) plus inferior hypogastric plexus block or botulinum toxin enabled us to prolong response time by more than 3.5 months. Conclusion: Treatment of the unresponsive patient should be interdisciplinary. Depending on the history and exploratory findings, therapy should preferably be combined with neuromodulators, myofascial pain therapies, and S3 PRF plus inferior hypogastric plexus blockade.
Comparative Evaluation of the Prognosis of Septic Shock Patients from Before to After the Onset of the COVID-19 Pandemic: A Retrospective Single-Center Clinical Analysis
Authors: Kim Jae Hwan , Ahn Chiwon , Namgung Myeong
Published: January 2022

In this study, we investigated the mortality of septic shock patients visiting emergency departments (ED) before and after the coronavirus disease (COVID-19) pandemic onset. We retrospectively reviewed medical records and National Emergency Department Information System data of septic shock patients who visited the ED of a tertiary medical center in South Korea from February 2019 to February 2021. Following the COVID-19 pandemic onset, revised institutional ED processes included a stringent isolation protocol for patients visiting the ED. The primary goal of this study was to determine the mortality rate of septic shock patients from before and after the onset of the COVID-19 pandemic. Durations of vasopressor use, mechanical ventilation, intensive care unit stay, and hospitalization were investigated. The mortality rates increased from 24.8% to 35.8%, before and after COVID-19-onset, but the difference was not statistically significant (p = 0.079). No significant differences in other outcomes were found. Multivariable analysis revealed that the Simplified Acute Physiology Score III (SAPS III) was the only risk factor for mortality (OR 1.07; 95% CI 1.04-1.10), whereas COVID-19 pandemic was not included in the final model. The non-significant influence of the COVID-19 pandemic on septic shock mortality rates in the present study belies the actual mortality-influencing potential of the COVID-19 pandemic.
Trading Activities and the Volatility of Return on Malaysian Crude Palm Oil Futures
Authors: Yeap Xiu Wei , Lean Hooi Hooi
Published: January 2022

Trading activities represent the flow of market information to the investors. This paper examines the effect of trading activities, i.e., trading volume and open interest, on the volatility of return for Malaysian Crude Palm Oil Futures. The GARCH model is applied by adding the expected and unexpected elements of trading activities (trading volume and open interest) as the independent variables. The results show that there is a negative contemporaneous relationship between the expected volume and volatility, but that a positive relationship exists between unexpected volume and volatility. On the contrary, the expected and unexpected open interest mitigate the volatility. Therefore, both trading volume and open interest should be considered together when information flows into the market.
Experimental Study on Atomization Characteristics of Gas–Liquid Two-Phase Flow Nozzle and Its Dust Removal Effect
Authors: Fang Xueming , Jiang Bingyou , Yuan Liang , Liang Yuxiang , Ren Bo , Tao Wenhan , Li Xianbao
Published: January 2022

An experimental study on the flow rate and atomization characteristics of a new gas–liquid two-phase flow nozzle was carried out to use high-concentration respirable dust in the workplace of high-efficiency sedimentation coal production based on the gas–liquid two-phase flow nozzle technology. The simulation roadway of dust fall in large coal mines was constructed, and the respirable rock dust produced by fully mechanized mining surfaces was chosen as the research object. The effects of humidity on the capture effect of respirable rock dust were analyzed in the experimental study. The results demonstrated that: (1) the distribution range of the particle size of fogdrops declines with the reduction in fogdrops D50, D[3,2] and D[4,3], which are produced by gas–liquid two-phase flow nozzles. (2) The initial ambient humidity in the simulated roadway was 64.8% RH. After the gas–liquid two-phase flow spray was started, the ambient humidity was elevated by 23.2 to 23.5% RH within 840s and tended to be stable and no longer grew after reaching 88.0–88.3% RH. The initial growth rate of the ambient humidity in the simulated roadway was high, and then was gradually slowed down. (3) Humidity is an important factor influencing the collection of respirable dust. The humidity at 10.0 m leeward of the dust-producing point was increased by 19.6% RH, and the sedimentation rate of respirable dust was increased by 6.73%; the two growth rates were 13.1% RH and 9.90% at 20.0 m; 16.4% RH and 15.42% at 30.0 m; 18.4% RH and 11.20% at 40.0 m. In practical applications of the gas–liquid two-phase flow nozzle in coal mining activities, attention shall be paid to not only the influences of its atomization characteristics on the capture effect of respirable dust but also the influences of the flow rate of the nozzle on the humidity of the working surface. Appropriate gas and water supply pressures shall be chosen according to the space and respirable dust concentration on the working surface to realize a better dust removal effect.
An Analytical Approach for Computing the Coefficient of Refrigeration Performance in Giant Inverse Magnetocaloric Materials
Authors: Bruno Nickolaus M. , Phillips Matthew R.
Published: January 2022

An analytical approach for computing the coefficient of refrigeration performance (CRP) was described for materials that exhibited a giant inverse magnetocaloric effect (MCE), and their governing thermodynamics were reviewed. The approach defines the magnetic work input using thermodynamic relationships rather than isothermal magnetization data discretized from the literature. The CRP was computed for only cyclically reversible temperature and entropy changes in materials that exhibited thermal hysteresis by placing a limit on their operating temperature in a thermodynamic cycle. The analytical CRP serves to link meaningful material properties in first-order MCE refrigerants to their potential work and efficiency and can be employed as a metric to compare the behaviors of dissimilar alloy compositions or for materials design. We found that an optimum in the CRP may exist that depends on the applied field level and Clausius–Clapeyron (CC) slope. Moreover, through a large literature review of NiMn-based materials, we note that NiMn(In/Sn) alloys offer the most promising materials properties for applications within the bounds of the developed framework.
Pilot-Scale Test Results of Electrodialysis Bipolar Membrane for Reverse-Osmosis Concentrate Recovery
Authors: Gazigil Leyla , Er Eren , Kestioğlu O. Erdem , Yonar Taner
Published: January 2022

In this study, it is aimed to investigate the potential of electrodialysis bipolar membrane (EDBM) systems for the recovery of the concentrate originating from an organized industrial estate (OIE) wastewater treatment system with reverse osmosis (RO). Acids and bases were obtained from a pilot-scale treatment plant as a result of the research. Furthermore, the sustainability and affordability of acids and bases obtained by EDBM systems were investigated. Six cycles were carried out in continuous-flow mode with the EDBM system as batch cycles in the disposal of the concentrate and the production of acids and bases with the EDBM system. For each cycle, the EDBM system was operated for 66, 48, 66, and 80 min, respectively, and the last two cycles were operated for a total of 165 min (70 + 90) with 5 min of waiting. In the EDBM system, a working method was determined such that the cycle flow rate was 180 L/hour, energy to be given to the system was 25 V, and the working pressure was in the range of 0.8–2.5 bar. In the six cycles with the EDBM system, the concentrate, acid and base, conductivity, pH, and pressure increase values were investigated depending on time. Throughout all these studies, the cycles were continued over the products formed in the acid and base chamber. As a result of all the cycles, acid (HCl) production at a level of 1.44% and base (NaOH) production at a level of 2% were obtained.
Sex-Dependent Social and Repetitive Behavior and Neurochemical Profile in Mouse Model of Autism Spectrum Disorder
Authors: Ferreira Helena , Sousa Ana Catarina , Sereno José , Martins João , Castelo-Branco Miguel , Gonçalves Joana
Published: January 2022

Autism spectrum disorder (ASD) is a neurodevelopmental condition characterized by deficits in social interaction, impaired communication, and repetitive behaviors. ASD presents a 3:1 ratio of diagnosed boys and girls, raising the question regarding sexual dimorphic mechanisms underlying ASD symptoms, and their molecular basis. Here, we performed in vivo proton magnetic resonance spectroscopy in juvenile male and female Tsc2+/− mice (an established genetic animal model of ASD). Moreover, behavior and ultrasonic vocalizations during social and repetitive tasks were analyzed. We found significant sexual dimorphisms in the levels of metabolites in the hippocampus and prefrontal cortex. Further, we observed that female mutant animals had a differential social behavior and presented an increase in repetitive behavior. Importantly, while mutant females displayed a more simplified communication during social tasks, mutant males exhibited a similar less complex vocal repertoire but during repetitive tasks. These results hint toward sex-dependent alterations in molecular and metabolic pathways, which can lead to the sexual dimorphic behaviors and communication observed in social and repetitive environments.
Precision and Advanced Nano-Phytopharmaceuticals for Therapeutic Applications
Authors: Lim Chooi Ling , Raju Chandramathi S. , Mahboob Tooba , Kayesth Sunil , Gupta Kamal K. , Jain Gaurav Kumar , Dhobi Mahaveer , Nawaz Muhammad , Wilairatana Polrat , de Lourdes Pereira Maria , Patra Jayanta Kumar , Paul Alok K. , Rahmatullah Mohammed , Nissapatorn Veeranoot
Published: January 2022

Phytopharmaceuticals have been widely used globally since ancient times and acknowledged by healthcare professionals and patients for their superior therapeutic value and fewer side-effects compared to modern medicines. However, phytopharmaceuticals need a scientific and methodical approach to deliver their components and thereby improve patient compliance and treatment adherence. Dose reduction, improved bioavailability, receptor selective binding, and targeted delivery of phytopharmaceuticals can be likely achieved by molding them into specific nano-formulations. In recent decades, nanotechnology-based phytopharmaceuticals have emerged as potential therapeutic candidates for the treatment of various communicable and non-communicable diseases. Nanotechnology combined with phytopharmaceuticals broadens the therapeutic perspective and overcomes problems associated with plant medicine. The current review highlights the therapeutic application of various nano-phytopharmaceuticals in neurological, cardiovascular, pulmonary, and gastro-intestinal disorders. We conclude that nano-phytopharmaceuticals emerge as promising therapeutics for many pathological conditions with good compliance and higher acceptance.
Vitamin D Deficiency Is Associated with Glycometabolic Changes in Nondiabetic Patients with Arterial Hypertension
Authors: Brosolo Gabriele , Da Porto Andrea , Bulfone Luca , Scandolin Laura , Vacca Antonio , Bertin Nicole , Vivarelli Cinzia , Sechi Leonardo A. , Catena Cristiana
Published: January 2022

Recent evidence indicates that mildly increased fasting and post-oral load blood glucose concentrations contribute to development of organ damage in nondiabetic patients with hypertension. In previous studies, vitamin D deficiency was associated with decreased glucose tolerance. The aim of this study was to examine the relationships between serum 25(OH)D levels and glucose tolerance and insulin sensitivity in hypertension. In 187 nondiabetic essential hypertensive patients free of cardiovascular or renal complications, we measured serum 25-hydroxyvitamin D (25(OH)D) and parathyroid hormone (PTH) and performed a standard oral glucose tolerance test (OGTT). Patients with 25(OH)D deficiency/insufficiency were older and had significantly higher blood pressure, fasting and post-OGTT (G-AUC) glucose levels, post-OGTT insulin (I-AUC), PTH levels, and prevalence of metabolic syndrome than patients with normal serum 25(OH)D. 25(OH)D levels were inversely correlated with age, blood pressure, fasting glucose, G-AUC, triglycerides, and serum calcium and PTH, while no significant relationships were found with body mass index (BMI), fasting insulin, I-AUC, HOMA index, and renal function. In a multivariate regression model, greater G-AUC was associated with lower 25(OH)D levels independently of BMI and seasonal vitamin D variations. Thus, in nondiabetic hypertensive patients, 25(OH)D deficiency/insufficiency could contribute to impaired glucose tolerance without directly affecting insulin sensitivity.