bluuebunny/crossref_metadata_2025 huggingface dataset:
The Future of Post-Industrial Landscapes in East Lisbon: The Braço de Prata Neighbourhood
Authors: Yagci Eser , Nunes da Silva Fernando
Published: April 2021

East Lisbon is being exposed to large-scale urban regeneration processes, where luxury residential projects and mixed-use spatial developments are already underway. Thus, it is a living laboratory for “smart”, “creative” and “green” projects, as well as related urban public space interventions. Braço de Prata is an urban space overlooked by developers, being surrounded by obsolete industrial buildings. Concerning the recent interest in international investments in brownfield regeneration and greenfield developments, it represents an attractive urban terrain as a post-industrial working-class neighbourhood, where “smart” and “green” suggest transforming space so that both new and old residents can live and work together and share public space regardless of analysis on their environmental recognitions. The aim of this paper is to present an empirical evaluation model that examines the possible impacts of environmental negligence through the reorganisation of the physical and social fabric. The analyses focus on dwellers’ moral understanding of their changing environment as site-specific domains to address the unique conditions that affect transiently defined presumptions about the collective needs. Taking an evaluative approach in the Braço de Prata case, this paper demonstrates the specific socio-ecological implications of urban inequality in post-industrial neighbourhoods that could be threatened by new decisions, both through urban planning approaches and instruments.
Considering Condensable Particulate Matter Emissions Improves the Accuracy of Air Quality Modeling for Environmental Impact Assessment
Authors: Choi Doo Sung , Youn Jong-Sang , Lee Im Hack , Choi Byung Jin , Jeon Ki-Joon
Published: April 2021

This study examines environmental impact assessment considering filterable particulate matter (FPM) and condensable particulate matter (CPM) to improve the accuracy of the air quality model. Air pollutants and meteorological data were acquired from Korea’s national monitoring station near a residential development area in the target district and background site. Seasonal emissions of PM2.5, including CPM, were estimated using the California puff (CALPUFF) model, based on Korea’s national emissions inventory. These results were compared with the traditional environmental impact assessment results. For the residential development area, the seasonal PM2.5 concentration was predicted by considering FPM and CPM emissions in the target area as well as the surrounding areas. In winter and spring, air quality standards were not breached because only FPM was considered. However, when CPM was included in the analysis, the results exceeded the air quality standards. Furthermore, it was predicted that air quality standards would not be breached in summer and autumn, even when CPM is included. In other words, conducting an environmental impact assessment on air pollution including CPM affects the final environmental decision. Therefore, it is concluded that PM2.5 should include CPM for greater accuracy of the CALPUFF model for environmental impact assessment.
Analysis of Mudstone Fracture and Precursory Characteristics after Corrosion of Acidic Solution Based on Dissipative Strain Energy
Authors: Dong Xu , Wu Yu , Cao Kewang , Muhammad Khan Naseer , Hussain Sajjad , Lee Seungyeon , Ma Chuan
Published: April 2021

The deformation and failure of rock materials are closely related to the strain energy characteristics during the loading process. These strain energy characteristics and rock properties are greatly affected when the rock is subjected to the acidic solution. To study the effects of chemical solutions with different pH on the mechanical properties and strain energy mechanism of mudstone, the chemical corrosion mudstone samples are subjected to a uniaxial loading testing machine (CN64 electro-hydraulic servo). The corrosive effects of the acidic solution on the porosity, strain energy characteristics, and failure mode of mudstone samples were thoroughly investigated. The findings of this research indicate that: (1) The rate of change in the porosity and chemical damage coefficient of rock samples after chemical corrosion decreases, which is closely linear with the increase of solution pH; (2) The total strain energy, elastic strain energy, and dissipative strain energy decrease with the increase of pH, and, as a result, it is proposed that the observed turning point of the proportion curve of dissipated strain energy from decline to rise is used as a precursor point of the rock failure; (3) The stress value of the failure precursor point increases and the strain value decreases with the increase in pH value. However, the ratio of the stress value of the failure precursor point to the peak stress hardly changes with pH value, and its value is about 0.883; and (4) Rock samples soaked in a weak acidic chemical solution (pH 7.3 and 5.3) are damaged by tensile crack, while rock samples soaked in a strong acidic chemical solution (pH 3.3 and 1.3) are mainly damaged by the combination of tensile and shear. The findings of this study can be used to provide an experimental and theoretical foundation for monitoring rock engineering disasters such as slope, tunnel, and coal mine failures.
High Voltage Electric Discharge for Recovery of Chlorogenic Acid from Tobacco Waste
Authors: Banožić Marija , Jozinović Antun , Grgić Jovana , Miličević Borislav , Jokić Stela
Published: April 2021

Three fractions of tobacco waste (scrap, dust and midrib) were subjected to a high voltage electric discharge (HVED) assisted extraction procedure under different experimental conditions: solvent:solid ratio (300, 500, 700 mL/g), frequency (40, 70, 100 Hz) and treatment time (15, 30, 45 min), in order to study the influence of these conditions on the content of chlorogenic acid. The content of chlorogenic acid ranged from 1.54 to 3.66 mg/100 g for scrap, from 1.90 to 2.97 mg/100 g for dust, and from 2.30 to 3.38 mg/100 g for midrib extract, showing a strong dependence on the applied process parameters. The temperature change and the change in pH and electrical conductivity of the extracts after high voltage discharge treatment were also observed. The studied process parameters showed a statistically significant effect on the chemical and physical properties of the extracts from tobacco waste as well as on the content of chlorogenic acid, indicating the potential of HVED assisted processes in the separation of chlorogenic acid from tobacco industry waste. Multiple regression analysis was used to fit the results for the chlorogenic acid to a second order polynomial equation and the optimum conditions were determined.
Development of Riparian and Groundwater-Dependent Ecosystem Assessments for National Forests in the Western U.S.
Authors: Driscoll Katelyn P. , Smith D. Max
Published: April 2021

In 2012, the U.S. Department of Agriculture adopted a new planning rule that outlined a process for developing, amending, and revising land management plans for the 155 National Forests, 20 National Grasslands, and one Tallgrass Prairie managed by the U.S. Forest Service. The rule outlines a framework with three phases: assessment, development/amendment/revision, and monitoring. We are assisting National Forests in the western U.S. with the first phase by completing a series of assessments of riparian and groundwater-dependent ecosystems. Here, we describe our methods and the lessons learned over the course of conducting assessments for seven National Forests. Per the requirements of the planning rule, we conduct a rapid assessment of ecological integrity that uses existing data to evaluate drivers, stressors, structure, function, composition, and connectivity. We have collaborated with National Forests, state agencies, and other research groups to obtain datasets representing various wetland landscape features. Our work supports the plan revision process, from assessment through plan approval, and informs future forest and project planning for the restoration and maintenance of structure, function, composition, and connectivity. We developed our assessment methods in collaboration with resource managers at the National Forest and regional level to ensure useful end products such as published technical reports, literature reviews, photo libraries, or collections of datasets related to riparian and groundwater-dependent ecosystems. Our approach and lessons learned throughout the process are relevant to other resource management planning applications, analyses of landscape condition, as well as assessments of other ecosystems, such as forests or grasslands.
Study of the Variability in Fatty Acids and Carotenoid Profiles: Laying the Ground for Tank Milk Authentication
Authors: Villar Ana , Vázquez-González Ibán , Vicente Fernando , Salcedo Gregorio , González Laura , Botana Adrián , Royo Luís José , Eguinoa Paola , Busqué Juan
Published: April 2021

This study analyzes 174 tank milk samples taken from 89 commercial farms located all along the Cantabrian Coast (Green Spain). Sampling was performed in two periods: autumn 2016 and spring 2017. A survey was carried out for every day of sampling to record the average lactating dairy cow production and its diet composition. For each sample, the fatty acid (FA) profile (49 FA plus its main relationships) and nine fat-soluble antioxidant (FSA) profiles (retinol (vitamin A), α- and Υ-tocopherol (vitamin E), all-trans-β-carotene, 9-cis-β-carotene, 13-cis-β-carotene, lutein, zeaxanthin, and β-cryptoxanthin) were determined. The milk production varied between 7.3 and 45.9 liters per cow per day, highlighting the diversity found among production systems. The milk fat content ranged from 2.64% to 4.38% and the protein content from 2.87% to 3.56%. Regarding the fatty acids profile, the percentage of saturated fat varied between 59.95% and 75.99%. The linolenic acid content fluctuated between 0.21 and 1.31 and rumenic acid ranged from 0.20 to 2.47 (g 100 g−1 total FA). The most important correlations between diet and milk FA were always related to the content of fresh grass and total forage (which is defined by both fresh and conserved forage derived from fresh grass (GCF)) in the diet. The content of vaccenic acid, linolenic acid, total omega-3, rumenic acid, and total CLA isomers showed the highest correlation with the proportion of fresh grass in the diet. The antioxidant contents were also highly variable, although correlations with dietary components were lower. The highest correlations were between total forage content (fresh grass (FG) plus GCF) and lutein, all-trans-β-carotene, and 13-cis-β-carotene. Diets without fresh grass had lower omega-3 content, CLA, vaccenic acid, lutein, all-trans-β-carotene, and 13-cis-β-carotene.
Smart Farming through Responsible Leadership in Bangladesh: Possibilities, Opportunities, and Beyond
Authors: Haque Amlan , Islam Nahina , Samrat Nahidul Hoque , Dey Shuvashis , Ray Biplob
Published: April 2021

Smart farming has the potential to overcome the challenge of 2050 to feed 10 billion people. Both artificial intelligence (AI) and the internet of things (IoT) have become critical prerequisites to smart farming due to their high interoperability, sensors, and cutting-edge technologies. Extending the role of responsible leadership, this paper proposes an AI and IoT based smart farming system in Bangladesh. With a comprehensive literature review, this paper counsels the need to go beyond the simple application of traditional farming and irrigation practices and recommends implementing smart farming enabling responsible leadership to uphold sustainable agriculture. It contributes to the current literature of smart farming in several ways. First, this paper helps to understand the prospect and challenges of both AI and IoT and the requirement of smart farming in a nonwestern context. Second, it clarifies the interventions of responsible leadership into Bangladesh’s agriculture sector and justifies the demand for sustainable smart farming. Third, this paper is a step forward to explore future empirical studies for the effective and efficient use of AI and IoT to adopt smart farming. Finally, this paper will help policymakers to take responsible initiatives to plan and apply smart farming in a developing economy like Bangladesh.
Integrated Healthcare and the Dilemma of Public Health Emergencies
Authors: Goniewicz Krzysztof , Carlström Eric , Hertelendy Attila J. , Burkle Frederick M. , Goniewicz Mariusz , Lasota Dorota , Richmond John G. , Khorram-Manesh Amir
Published: April 2021

Traditional healthcare services have demonstrated structural shortcomings in the delivery of patient care and enforced numerous elements of integration in the delivery of healthcare services. Integrated healthcare aims at providing all healthcare that makes humans healthy. However, with mainly chronically ill people and seniors, typically suffering from numerous comorbidities and diseases, being recruited for care, there is a need for a change in the healthcare service structure beyond direct-patient care to be compatible in peacetime and during public health emergencies. This article’s objective is to discuss the opportunities and obstacles for increasing the effectiveness of healthcare through improved integration. A rapid evidence review approach was used by performing a systematic followed by a non-systematic literature review and content analysis. The results confirmed that integrated healthcare systems play an increasingly important role in healthcare system reforms undertaken in European Union countries. The essence of these changes is the transition from the episodic treatment of acute diseases to the provision of coordinated medical services, focused on chronic cases, prevention, and ensuring patient continuity. However, integrated healthcare, at a level not yet fully defined, will be necessary if we are to both define and attain the integrated practice of both global health and global public health emergencies. This paper attains the necessary global challenges to integrate healthcare effectively at every level of society. There is a need for more knowledge to effectively develop, support, and disseminate initiatives related to coordinated healthcare in the individual healthcare systems.
Factors Influencing Entrepreneurial Intention of University Students in China: Integrating the Perceived University Support and Theory of Planned Behavior
Authors: Su Yushun , Zhu Zeren , Chen Jingwen , Jin Yuanqing , Wang Ting , Lin Chien-Liang , Xu Danying
Published: April 2021

Entrepreneurial education has always played an important role in developing entrepreneurship. In recent years, China has been emphasizing the integration of innovative entrepreneurship concepts into university curricula. Entrepreneurial education can also contribute to the sustainability of business development. In the context of “mass entrepreneurship and innovation”, entrepreneurial education is important to promote the restructuring of the economic and business model, enhance the development momentum, and follow the innovation-driven development path. However, whether the promotion of entrepreneurial education will lead to entrepreneurial intentions of college students, and thus, specific proposals for the promotion of the entrepreneurial education model remains unclear. Therefore, this study adopted the theory of planned behavior perspective coupled with perceived university support to extend the theory of planned behavior framework and explain the effect of such support on student entrepreneurial intention. The study results revealed that perceived university support significantly affected student attitude toward entrepreneurship, which signaled universities’ critical role in establishing entrepreneurial spirit in students. A significant effect on behavioral control was also observed for perceived university support. Regarding the effects of perceived university support on attitude toward entrepreneurship and behavioral control, the results revealed the effect was far greater on attitude than on behavioral control. Such intention was not directly affected by subjective norms. Therefore, a scientifically rational entrepreneurship course system is critical for effective entrepreneurship education. Overall, encouraging students to engage in the continuous process of entrepreneurship through entrepreneurship courses can concurrently benefit overall economic and social development.
Preparation of Electric- and Magnetic-Activated Water and Its Influence on the Workability and Mechanical Properties of Cement Mortar
Authors: Zhao Kaiyue , Zhang Peng , Wang Bing , Tian Yupeng , Xue Shanbin , Cong Yuan
Published: April 2021

Cement-based materials prepared with activated water induced by a magnetic field or electric field represent a possible solution to environmental issues caused by the worldwide utilization of chemical admixtures. In this contribution, electric- and magnetic-activated water have been produced. The workability and mechanical properties of cement mortar prepared with this activated water have been investigated. The results indicate that the pH and absorbance (Abs) values of the water varied as the electric and magnetic field changed, and their values increased significantly, exhibiting improved activity compared with that of the untreated water. In addition, activated water still retains activity within 30 min of the resting time. The fluidity of the cement paste prepared with electric-activated water was significantly larger than that of the untreated paste. However, the level of improvement differed with the worst performance resulting from cement paste prepared with alternating voltage activated water. In terms of mechanical properties, both compressive strength and flexural strength obtained its maximum values at 280 mT with two processing cycles. The compressive strength increased 26% as the curing time increased from 7 days to 28 days and flexural strength increased by 31%. In addition, through the introduction of magnetic-activated water into cement mortar, the mechanical strength can be maintained without losing its workability when the amount of cement is reduced.
Analysis of Corporate Social Responsibility Execution Effects on Purchase Intention with the Moderating Role of Customer Awareness
Authors: Zhang Qingyu , Ahmad Sohail
Published: April 2021

Corporate social responsibility (CSR) in management domains is a well-known concept that links corporate interests and environmental/community values. CSR is considered a strategic policy that offers environmental and social competitive advantages. Organizations consider that CSR-based goodwill provides a tactical competitive edge and sustainable growth. The goal of this paper is to show how CSR programs affect consumers’ purchasing intention in the context of Pakistan. In addition, the effect of customer awareness has been studied as a moderator between CSR and purchasing intention. To this end, the study has conducted a survey and gathered Pakistani customers’ responses, and structural equation modeling has been used to evaluate the results. The study concludes that CSR activities favorably affect customer purchasing intentions directly as well as indirectly through improving brand image and trust, and customer awareness of CSR activities plays a moderating role. The implications and future research directions are discussed.
A Methodology for Assessing the Impact of Living Labs on Urban Design: The Case of the Furnish Project
Authors: Aquilué Inés , Caicedo Angélica , Moreno Joan , Estrada Miquel , Pagès Laia
Published: April 2021

This paper presents a framework to support the assessment of urban design projects through Urban Living Labs (ULLs). The framework is based on the Tactical Urbanism (TU) practices and involves the use of Mobile Urban Elements (MUE) in uncertain and potentially confusing conditions (e.g., the COVID-19 context). The methodology includes the application of the Four-Phase Model (problem and ideation; development; implementation, testing and assessment; final proposal) and a quantitative and qualitative assessment. The proposed assessment criteria were developed through an evaluation according to three aspects: (1) feasibility impact; (2) social impact; and (3) spatial impact. The methodology was applied to Furnish, an urban design project based on a ULL and prototyping, which was recently developed in five European cities. The empirical results, obtained using the impact analysis, indicate that the prototypes developed in the project are transferable to other cities and generate social interaction in public spaces. The applied research showed that the Four-Phase Model may be used as a new and improved iterative design process: the LOOP Scheme. The application of this assessment methodology to ULLs may provide valuable information for the future planning of urban interventions in public spaces.
A Multilevel Model of Environmentally Specific Social Identity in Predicting Environmental Strategies: Evidence from Technology Manufacturing Businesses
Authors: Huang Stanley Y. B. , Ting Chih-Wen , Fei Yu-Ming
Published: April 2021

This study proposed a multilevel model of environmentally specific social identity based on upper echelons theory and examined how environmentally specific transformational leadership influenced the environmentally specific social identity of the top management team (TMT), which consequently influenced a corporation’s choices of proactive environmental strategies. Besides, the environmentally specific transformational leadership atmosphere at the TMT level also influenced the environmentally specific social identity atmosphere at the TMT level, which consequently influenced a corporation’s choices of proactive environmental strategies at the same time. In particular, this study proposed a novel concept–environmentally specific social identity based on social identity theory, including environmentally specific self-categorization, environmentally specific affective commitment, environmentally specific self-esteem. This study employed a hierarchical linear model and collected longitudinal data of 210 chief executive officers with their 840 members of TMTs at technology manufacturing businesses of Greater China at three waves over six months to analyze the theoretical model. This study found that individual-level environmentally specific transformational leadership and TMT-level environmentally specific transformational leadership (atmosphere) influenced individual-level environmentally specific social identity and TMT-level environmentally specific social identity (atmosphere), which consequently influenced proactive environmental strategies. These findings provide theoretical insights for the field of sustainable development that can advance the literature on proactive environmental strategies.
A New Generalization of the Generalized Inverse Rayleigh Distribution with Applications
Authors: Bakoban Rana Ali , Al-Shehri Ashwaq Mohammad
Published: April 2021

In this article, a new four-parameter lifetime model called the beta generalized inverse Rayleigh distribution (BGIRD) is defined and studied. Mixture representation of this model is derived. Curve’s behavior of probability density function, reliability function, and hazard function are studied. Next, we derived the quantile function, median, mode, moments, harmonic mean, skewness, and kurtosis. In addition, the order statistics and the mean deviations about the mean and median are found. Other important properties including entropy (Rényi and Shannon), which is a measure of the uncertainty for this distribution, are also investigated. Maximum likelihood estimation is adopted to the model. A simulation study is conducted to estimate the parameters. Four real-life data sets from difference fields were applied on this model. In addition, a comparison between the new model and some competitive models is done via information criteria. Our model shows the best fitting for the real data.
Integrated Policy Solutions for Water Scarcity in Agricultural Communities of the American Southwest
Authors: Langarudi Saeed P. , Maxwell Connie M. , Fernald Alexander G.
Published: April 2021

The conventional approach of policy interventions in water management that focus on the portions of the system that directly relate to water often lead to unintended consequences that potentially exacerbate water scarcity issues and present challenges to the future viability of many rural agricultural communities. This paper deploys a system dynamics model to illustrate how expanding the policy space of hydrology models to include socioeconomic feedbacks could address these challenges. In this regard, policies that can potentially mitigate general water scarcity in a region of the American Southwest in southern New Mexico are examined. We selected and tested policies with the potential to diminish water scarcity without compromising the system’s economic performance. These policies included supporting choices that reduce or limit the expansion of water-intensive crops, promoting workforce participation, encouraging investment in capital, and regulating land use change processes. The simulation results, after the proposed boundary expansion, unveiled intervention options not commonly exercised by water decision-makers, bolstering the argument that integrated approaches to water research that include socioeconomic feedbacks are crucial for the study of agricultural community resilience.
Differentiation, Quantification and Identification of Abrin and Abrus precatorius Agglutinin
Authors: Worbs Sylvia , Kampa Bettina , Skiba Martin , Hansbauer Eva-Maria , Stern Daniel , Volland Hervé , Becher François , Simon Stéphanie , Dorner Martin B. , Dorner Brigitte G.
Published: April 2021

Abrin, the toxic lectin from the rosary pea plant Abrus precatorius, has gained considerable interest in the recent past due to its potential malevolent use. However, reliable and easy-to-use assays for the detection and discrimination of abrin from related plant proteins such as Abrus precatorius agglutinin or the homologous toxin ricin from Ricinus communis are sparse. To address this gap, a panel of highly specific monoclonal antibodies was generated against abrin and the related Abrus precatorius agglutinin. These antibodies were used to establish two sandwich ELISAs to preferentially detect abrin or A. precatorius agglutinin (limit of detection 22 pg/mL for abrin; 35 pg/mL for A. precatorius agglutinin). Furthermore, an abrin-specific lateral flow assay was developed for rapid on-site detection (limit of detection ~1 ng/mL abrin). Assays were validated for complex food, environmental and clinical matrices illustrating broad applicability in different threat scenarios. Additionally, the antibodies turned out to be suitable for immuno-enrichment strategies in combination with mass spectrometry-based approaches for unambiguous identification. Finally, we were able to demonstrate for the first time how the developed assays can be applied to detect, identify and quantify abrin from a clinical sample derived from an attempted suicide case involving A. precatorius.
The Contribution of Urban Morphology to the Formation of the Microclimate in Compact Urban Cores: A Study in the City Center of Thessaloniki
Authors: Kouklis Georgios-Rafail , Yiannakou Athena
Published: April 2021

The purpose of this paper is to investigate the contribution of urban morphology to the formation of microclimatic conditions prevailing within urban outdoor spaces. We studied the compact form of a city and examined, at a detailed, street plan level, elements related to air temperature, urban ventilation, and the individual’s thermal comfort. All elements examined are directly affected by both the urban form and the availability of open and green spaces. The field study took place in a typical compact urban fabric of an old city center, the city center of Thessaloniki, where we investigated the relationship between urban morphology and microclimate. Urban morphology was gauged by examining the detailed street plan, along with the local building patterns. We used a simulation method based on the ENVI-met© software. The findings of the field study highlight the fact that the street layout, the urban canyon, and the open and green spaces in a compact urban form contribute decisively both to the creation of the microclimatic conditions and to the influence of the bioclimatic parameters.
Modelling Urban Tourism in Historic Southeast Asian Cities
Authors: Ong Jackie Lei Tin , Smith Russell Arthur
Published: April 2021

Older cities with significant physical historic resources have become increasingly important centers for urban tourism, though contemporary attractions and events are often important in their own right. It is acknowledged that urban areas are multifaceted agglomerations where tourism complements other economic sectors and competes with them for limited resources. A limited investigation of the temporal dynamics of change of tourism in cities has been undertaken in the several countries in Europe and North America. Much less has been studied for the countries of Southeast Asia where tourism has expanded rapidly over the past several decades, a trend that is projected to continue. Urban tourism has and will continue to be important in Southeast Asia. This paper reports on the spatial modeling of the evolution of tourism in two historic cities in Southeast Asia that seeks to better understand the dynamics of temporal change of tourism within their respective urban contexts. The cases of Bangkok and Jakarta have been compared with the Ashworth and Tunbridge model to identify conformity and otherwise of Asian cases with the European theory.
Recombinant Pseudorabies Virus with TK/gE Gene Deletion and Flt3L Co-Expression Enhances the Innate and Adaptive Immune Response via Activating Dendritic Cells
Authors: Yao Lun , Hu Qiao , Chen Siqi , Zhou Tong , Yu Xuexiang , Ma Hailong , H. Ghonaim Ahmed. , Wu Hao , Sun Qi , Fan Shengxian , He Qigai
Published: April 2021

Owing to viral evolution and recombination, emerging pseudorabies virus (PRV) strains have caused unprecedented outbreaks in swine farms even when the pigs were previously vaccinated, which might indicate that traditional vaccines were unable to provide effective protection. The development of safe and efficacious vaccines presents prospects to minimize the clinical signs and eventually eradicate the infection. In this study, we used an emerging PRV strain, HNX, as the parental strain to construct a recombinant PRV with TK/gE gene deletion and Fms-related tyrosine kinase 3 ligand (Flt3L) expression, named HNX-TK−/gE−-Flt3L. HNX-TK−/gE−-Flt3L enhanced the maturation of bone marrow derived dendritic cells (DCs) in vitro. Significantly more activated DCs were detected in HNX-TK−/gE−-Flt3L-immunized mice compared with those immunized with HNX-TK−/gE−. Subsequently, a remarkable increase of neutralizing antibodies, gB-specific IgG antibodies, and interferon-gamma (IFN-γ) was observed in mice vaccinated with HNX-TK−/gE−-Flt3L. In addition, a lower mortality and less histopathological damage were observed in HNX-TK−/gE−-Flt3L vaccinated mice with upon PRV lethal challenge infection. Taken together, our results revealed the potential of Flt3L as an ideal adjuvant that can activate DCs and enhance protective immune responses and support the further evaluation of HNX-TK−/gE−-Flt3L as a promising PRV vaccine candidate.
When the COVID-19 Pandemic Surges during Influenza Season: Lessons Learnt from the Sentinel Laboratory-Based Surveillance of Influenza-Like Illness in Lombardy during the 2019–2020 Season
Authors: Galli Cristina , Pellegrinelli Laura , Bubba Laura , Primache Valeria , Anselmi Giovanni , Delbue Serena , Signorini Lucia , Binda Sandro , Cereda Danilo , Gramegna Maria , Pariani Elena ,   The ILI Sentinel Physicians Group
Published: April 2021

This paper outlines the role of Lombardy’s regional influenza reference laboratory (Northern Italy) in the surveillance of influenza-like illnesses (ILIs) in monitoring SARS-CoV-2 circulation by analyzing 631 consecutive nasopharyngeal swabs (NPSs) collected from ILI outpatients by sentinel physicians during the 2019–2020 season. The samples were tested by specific real-time RT-PCRs targeting SARS-CoV-2, influenza viruses, and RSVs. Results: Of these NPSs, 31% tested positive for influenza viruses, 10% for SARS-CoV-2, and 7% for RSV. No coinfections were detected. Influenza viruses and RSVs circulated throughout the surveillance period until the end of February (week 9-2020), when they suddenly ceased to circulate seven weeks earlier than during the previous five influenza seasons. After the first detection of SARS-CoV-2 in our ILI outpatients at the beginning of March (week 10-2020), SARS-CoV-2 remained the only virus identified throughout the surveillance period. Patients ≥ 65 years had a 3.2-fold greater risk of being infected with SARS-CoV-2, while school-age children (5–14 years) and children < 5 years proved to be the age groups most at risk of contracting influenza viruses and RSV, respectively. Our experience demonstrates that laboratory-based ILI surveillance networks are essential for identifying SARS-CoV-2 cases that would otherwise remain undetected, in order to stop their spread within our communities.
Genetic Characterization of a Neurovirulent West Nile Virus Variant Associated with a Fatal Great Grey Owl Infection
Authors: Peňazziová Katarína , Korytár Ľuboš , Pastorek Patrik , Pistl Juraj , Rusňáková Diana , Szemes Tomáš , Čabanová Viktória , Ličková Martina , Boršová Kristína , Klempa Boris , Csank Tomáš
Published: April 2021

This study reports on a fatal case of a captive great grey owl infected with the West Nile virus (WNV) in the zoological garden Košice, eastern Slovakia (Central Europe). The tissue samples of the dead owl were used for virus isolation and genetic characterization. The novel isolate is genetically closer to Hungarian, Greek, and Bulgarian strains from the central/southern European clade of lineage 2 than to the strains previously isolated in Slovakia. Interestingly, it carries NS3-249P, a molecular virulence determinant associated with higher neurovirulence, which has not previously been observed in Slovakia. Subsequent serological investigation of the captive owls revealed additional seropositive animals, indicating local WNV transmission. Although no WNV-positive mosquitoes were found, the presence of the WNV principal vector Culex pipiens complex together with the described fatal case and further serological findings indicate an endemic focus of bird-neurovirulent WNV variant in the area.
Hematological Profile of Pregnant Women with Suspected Zika Virus Infection Followed Up at a Referral Service in Manaus, Brazil
Authors: Andrade Anny Beatriz Costa Antony de , Gonçalves Maria Jacirema Ferreira , Redivo Elijane de Fátima , Alecrim Maria das Graças Costa , Martinez-Espinosa Flor Ernestina
Published: April 2021

The purpose of this paper is to describe the hematological profile of pregnant women with suspected Zika virus (ZIKV) infection followed up at a reference service for infectious diseases in Manaus, Brazil, through a clinical, epidemiological, cross-sectional study of pregnant women with an exanthematic manifestation who looked for care between 2015 and 2017. The participants were 499 pregnant women, classified into four subgroups, according to laboratory confirmation of infections: ZIKV-positive; ZIKV-positive and positive for another infection; positive for another infection but not ZIKV-positive; and not positive for any of the infections investigated. Hematological parameters were analyzed descriptively. The association between maternal infection and the hematological profile, along with the association between the maternal hematological profile and the gestational outcome, were tested. Similar hematic and platelet parameters were observed among pregnant women. However, a significant association was observed between low maternal lymphocyte count and a positive diagnosis for ZIKV (p < 0.001). The increase in maternal platelet count and the occurrence of unfavorable gestational outcome were positively associated. A similar hematic and platelet profile was identified among pregnant women, differing only in the low lymphocyte count among ZIKV-positive pregnant women. Regarding gestational outcomes, in addition to the damage caused by ZIKV infection, altered maternal platelets may lead to unfavorable outcomes, with the need for adequate follow-up during prenatal care.
Phase I Trial Evaluating the Safety and Immunogenicity of Candidate TB Vaccine MVA85A, Delivered by Aerosol to Healthy M.tb-Infected Adults
Authors: Riste Michael , Marshall Julia , Satti Iman , Harris Stephanie , Wilkie Morven , Lopez Ramon Raquel , Wright Danny , Wittenberg Rachel , Vermaak Samantha , Powell Doherty Rebecca , Lawrie Alison , Conlon Christopher , Cosgrove Catherine , Gleeson Fergus , Lipman Marc , Moss Paul , Perrin Felicity , Dedicoat Martin , Bettinson Henry , McShane Helen
Published: April 2021

The immunogenicity of the candidate tuberculosis (TB) vaccine MVA85A may be enhanced by aerosol delivery. Intradermal administration was shown to be safe in adults with latent TB infection (LTBI), but data are lacking for aerosol-delivered candidate TB vaccines in this population. We carried out a Phase I trial to evaluate the safety and immunogenicity of MVA85A delivered by aerosol in UK adults with LTBI (NCT02532036). Two volunteers were recruited, and the vaccine was well-tolerated with no safety concerns. Aerosolised vaccination with MVA85A induced mycobacterium- and vector-specific IFN-γ in blood and mycobacterium-specific Th1 cytokines in bronchoalveolar lavage. We identified several important barriers that could hamper recruitment into clinical trials in this patient population. The trial did not show any safety concerns in the aerosol delivery of a candidate viral-vectored TB vaccine to two UK adults with Mycobacterium tuberculosis (M.tb) infection. It also systemically and mucosally demonstrated inducible immune responses following aerosol vaccination. A further trial in a country with higher incidence of LTBI would confirm these findings.
On a Discrete SEIR Epidemic Model with Two-Doses Delayed Feedback Vaccination Control on the Susceptible
Authors: De la Sen Manuel , Alonso-Quesada Santiago , Ibeas Asier , Nistal Raul
Published: April 2021

A new discrete susceptible-exposed-infectious-recovered (SEIR) epidemic model is presented subject to a feedback vaccination effort involving two doses. Both vaccination doses, which are subject to a non-necessarily identical effectiveness, are administrated by respecting a certain mutual delay interval, and their immunity effect is registered after a certain delay since the second dose. The delays and the efficacies of the doses are parameters, which can be fixed in the model for each concrete experimentation. The disease-free equilibrium point is characterized as well as its stability properties, while it is seen that no endemic equilibrium point exists. The exposed subpopulation is supposed to be infective eventually, under a distinct transmission rate of that of the infectious subpopulation. Some simulation examples are presented by using disease parameterizations of the COVID-19 pandemic under vaccination efforts requiring two doses.
The Effect of Vaccination with Neospora caninum Live-Frozen Tachyzoites on Abortion Rates of Naturally Infected Pregnant Cows
Authors: Mazuz Monica L. , Leibovitz Benjamin , Savitsky Igor , Blinder Elena , Yasur-Landau Daniel , Lavon Yaniv , Sharir Binyamin , Tirosh-Levy Sharon
Published: April 2021

Neosporosis is a major cause of abortions in cattle worldwide. Recently a live attenuated vaccine showing promising results in preventing abortions, when administered at mid-pregnancy to seropositive cows, was developed. In this study, vaccination with 2 × 108 live frozen N. caninum tachyzoites (NcIs491) was used to immunize naturally infected seropositive pregnant dairy dams. The study was performed under field conditions in four herds, and a follow-up of three subsequent pregnancies was analyzed. A total of 1136 cows were serologically examined. Total seroprevalence was 41.4%, with 25.1% of the cows having titers of 1:800 or higher. Abortion rates were significantly higher in cows with high antibody titers (≥1:800) for two consecutive pregnancies. Vaccination was administered to 114 out of 285 cows with antibody titers higher than 1:800. Immunization resulted in lower abortion rates at three of the farms. Vaccine efficacy ranged from −19.8% to 75% at different farms, with overall efficacy of 28.4% in all four farms and overall efficacy of 58.2% in the three farms with positive results. Our results showed different vaccine efficacy in studied farms, suggesting that frozen live vaccination may generally be an effective method to control neosporosis in cattle.
A Case of Infectious Laryngotracheitis in an Organic Broiler Chicken Farm in Greece
Authors: Tsiouris Vasileios , Mavromati Natalia , Kiskinis Konstantinos , Mantzios Tilemachos , Homonnay Zalan G. , Mato Tamas , Albert Mihaly , Kiss Istvan , Georgopoulou Ioanna
Published: April 2021

Infectious laryngotracheitis is an economically significant viral disease of chickens, that mainly affects the upper respiratory tract, and is present worldwide. This case reports the first outbreak of infectious laryngotracheitis in a four-week-old organic broiler farm and surrounding flocks in Greece, with typical clinical symptoms and lesions, allegedly provoked by a wild strain of infectious laryngotracheitis virus. Our findings contradict the general perception indicating that the disease appears mainly in older birds and that vaccine strains are the primary cause of infectious laryngotracheitis outbreaks in most continents. A recombinant vectored vaccine was administered, supplementary to biosecurity measures, containing the viral spread. The responsible strain was potentially circulating in the area; therefore, an industry-wide holistic approach was applied, including the vaccination of neighboring broilers and breeders with the same vaccine, the rapid molecular diagnosis of the disease, and strict biosecurity protocols. The results of this holistic effort were effective because, following the application of vaccine and management protocols, manifestations of the disease in regional flocks dropped significantly, and there was no recurrence to date. These findings suggest that vaccination protocols should be modified, especially for organic broilers, to include vaccination against infectious laryngotracheitis.
Rainwater Management in Urban Areas
Authors: Helmreich Brigitte
Published: April 2021

Rising levels of impervious surfaces in densely populated cities and climate change-related weather extremes such as heavy rain events or long dry weather periods provide us with new challenges for sustainable stormwater management in urban areas. The Special Issue consists of nine articles and a review and focuses on a range of relevant issues: different aspects and findings of stormwater runoff quantity and quality, including strategies and techniques to mitigate the negative effects of such climate change impacts hydraulically, as well as lab-scale and long-term experience with pollutants from urban runoff and the efficiency of stormwater quality improvement devices (SQIDs) in removing them. Testing procedures and protocols for SQIDs are also considered. One paper analyses the clogging of porous media in the use of stormwater for managed aquifer recharge. The Special Issue demonstrates the importance and timeliness of the topic of sustainable rainwater management, especially with regard to growing cities and the challenges posed by climate change.
The Performance of Physically Based and Conceptual Hydrologic Models: A Case Study for Makkah Watershed, Saudi Arabia
Authors: Al-Areeq Ahmed M. , Al-Zahrani Muhammad A. , Sharif Hatim O.
Published: April 2021

Population growth and land use modification in urban areas require the use of accurate tools for rainfall-runoff modeling, especially where the topography is complex. The recent improvement in the quality and resolution of remotely sensed precipitation satisfies a major need for such tools. A physically-based, fully distributed hydrologic model and a conceptual semi-distributed model, forced by satellite rainfall estimates, were used to simulate flooding events in a very arid, rapidly urbanizing watershed in Saudi Arabia. Observed peak discharge for two flood events was used to compare hydrographs simulated by the two models, one for calibration and one for validation. To further explore the effect of watershed heterogeneity, the hydrographs produced by three implementations of the conceptual were compared against each other and against the output of the physically-based model. The results showed the ability of the distributed models to capture the effect of the complex topography and variability of land use and soils of the watershed. In general, the GSSHA model required less calibration and performed better than HEC-HMS. This study confirms that the semi-distributed HEC-HMS model cannot be used without calibration, while the GSSHA model can be the best option in the case of a lack of data. Although the two models showed good agreement at the calibration point, there were significant differences in the runoff, discharge, and infiltration values at interior points of the watershed.
Extraction and Purification of Phosphorus from the Ashes of Incinerated Biological Sewage Sludge
Authors: Baldi Marco , Martinotti Andrea , Sorlini Sabrina , Katsoyiannis Ioannis , Abbà Alessandro , Carnevale Miino Marco , Collivignarelli Maria
Published: April 2021

Phosphorus depletion represents a significant problem. Ash of incinerated biological sewage sludge (BSS) contains P, but the presence of heavy metals (e.g., Fe and Al) is the main issue. Based on chemical characterization by SEM-EDS, ED-XRF and ICP-OES techniques, the characteristics and P content of bottom ash (BA) and fly ash (FA) of incinerated BSS were very similar. On BA, P extraction carried out in counter- current with an S:L ratio of 1:10 and H2SO4 0.5 M led to better extraction yields than those of a similar test with H2SO4 1 M and an S:L ratio of 1:5 (93% vs. 86%). Comparing yields with H2SO4 0.5 M (S:L ratio of 1:10), the counter-current method gave better results than those of the crossflow method (93% vs. 83.9%), also improving the performance obtained with HCl in crossflow (93% vs. 89.3%). The results suggest that the purification of the acid extract from heavy metals with pH variation was impractical due to metal precipitation as phosphates. Extraction with H2SO4 and subsequent treatment with isoamyl alcohol represented the best option to extract and purify P, leading to 81% extraction yields of P with low amounts of metals.