bluuebunny/crossref_metadata_2025 huggingface dataset:
Intelligent Imaging Technology Implementation as Terrorism Prevention in Retail Sectors in 21st Century
Authors: Foster Bob , Johansyah Muhamad Deni
Published: November 2020

Terrorism is a very dangerous thing and has become a threat both on a national and international scale. Understanding terrorism seeks to instill the seeds of hostility towards a group, government agencies and even the state. Before entering the 21st century terror attacks are blatant in the business sector by sabotaging and damaging public facilities and vital objects. These physical attacks can be prevented by recording personal identities or conducting regular security patrols and placing a large number of security personnel in places of business and places that are considered strategic. However, this is still not optimal where the level of security and the number of personnel are limited in monitoring the place for a long time and are sustainable. The terrorist attacks in the 21st century are different and require different handling. Attacks can be physical or non-physical. On non-physical objects attacks are usually carried out by hacking networks that can damage internet network connections in one area and even one country. Ways to prevent attacks that are physical in nature by utilizing computer vision technology capabilities that are used to record all civil society. An additional feature in this technology lies in the face recognition and pattern matching techniques where a person's face will be searched for unique features. Then from these data will be biometric data, namely data that will not change and disappear. Computer vision algorithms can detect a person's face or object even though it has been modified or changed in physical and color conditions. The advantages of these algorithms are effectively used to record visitor identities and are optimal in recognizing an object with an accuracy of up to 90%. This system is able to help and improve a security feature of a business and tourist area.
Electronic Data Interchange (EDI) Applications Use the Decision Tree Method to Determine Vendor Recommendations
Authors: Siki Mariana Rospilinda , Harani Nisa Hanum , Prianto Cahyo
Published: November 2020

Electronic Data Interchange (EDI) is an electronic data exchange mechanism between a company and another company or Business to Business (B2B) in a supply chain cycle. In this study, EDI's role in managing the procurement of goods as well as the EDI model has been applied. Determination of vendor recommendations is one element of vendor performance evaluation of the procurement process. Lack of information and analysis obtained by PT. Cinovasi Rekaprima makes it difficult to predict vendor recommendations. Predicted vendor recommendations can help the Procurement Division in developing appropriate strategies to determine recommended vendors. This problem can be applied to data mining techniques to make predictions using the classification method. Decision Tree is a method that converts facts into decision trees that represent rules that can be interpreted by humans. Attributes that influence the determination of vendor recommendations consist of the availability of goods, services, ease of ordering and product quality. Sample data obtained directly from the Procurement Division of PT. Cinovasi Rekaprima is primary data in the form of vendor data (quotation) and secondary data in the form of vendor performance evaluation forms. The result of the EDI application is a classification consisting of 2 classes, namely recommended vendors and non-recommended vendors and the Procurement Division can use it for decision making to determine the right vendor, so that the procurement process becomes easier and increases company profitability. The testing model uses k-fold cross-validation with the k value is 1 to 10 fold. This application can determine vendor recommendations with the highest accuracy 87.00 % on k-3 and k-5 fold.
Pola Asuh Orangtua Bagi Anak yang Mengalami Gangguan Berbicara di Desa Kota Tanah Kecamatan Semendawai Timur Kabupaten Oku Timur
Authors: Tifani Yolanda , Lian Bukman , Sinaga Santa Idaya
Published: November 2020

Pola asuh yang digunakan orang tua dalam mendidik anak yang mengalami gangguan berbicara itu juga bisa sangat mempengaruhi dan berdampak pada kepribadian anak nantinya. Tujuan penelitian ini untuk mengetahui dampak dan cara mengatasi anak yang mengalami gangguan berbicara. Metode penelitian yang digunakan dalam penelitian ini adalah studi kasus, pada penelitian ini objek penelitiannya adalah anak yang mengalami gangguan berbicara yang terdiri dari 1 (satu) orang. Data yang akan dianalisis adalah observasi,wawancara dan dokumentasi. Hasil penelitian ini menunjukkan bahwa pola asuh berdampak negative bagi anak yang mengalami gangguan berbicara yaitu masalah pada pertumbuhan dan perkembangan anak yang akan berbeda dengan anak pada umumnya. Dampak yang anak alami adalah kurangnya bersosialisasi dengan teman atau lingkungan sekitarnya karena anak yang mengalami gangguan berbicara ini sering kali dikucilkan oleh teman-temannya sehingga membuat anak tersebut lebih murung dan pendiam..
Pengembangan Media Buku Dongeng Fabel untuk Mengenalkan Keaksaraan Anak Usia 5-6 Tahun Di Wonorejo Glagah
Authors: Izzah Lailatul , Adhani Dwi Nurhayati , Fitroh Siti Fadjryana
Published: November 2020

Penelitian ini bertujuan untuk membuat dan mengembangkan media pembelajaran dongeng fabel Elang dan Kelinci di Wonorejo Kecamatan Glagah Kabupaten Lamongan yang dapat digunakan untuk mengenalkan kemampuan keaksaraan anak usia 5-6 tahun sehingga membantu pengajar dalam menyampaikan materi pelajaran. Media pembelajaran ini dibuat dengan menggambar  isi dalam dongeng dan menggabungkan gambar dan teks dengan microsoft word kemudian dicetak dalam bentuk buku, selain itu juga untuk mengetahui kelayakan media pembelajaran ini. Penelitian ini dilaksanakan di Wonorejo Kecamatan Glagah Kabupaten Lamongan, merupakan penelitian pengembangan (Reseach and Development). Obyek penelitian adalah media pembelajaran dongeng fabel Elang dan Kelinci. Tahap penelitian meliputi (1) Analysis (Analisis), (2) Design (Desain), (3) Develpment (Pengembangan), (4) Implementation (Implementasi), (5) Evaluation (Evaluasi). Teknik pengumpulan data yang digunakan adalah menggunakan angket dan kuesioner yang dianalisis secara kuantitatif dan kualitatif. Subyek penelitian adalah anak usia 5-6 tahun di Wonorejo Glagah Lamongan. Hasil penelitian ini adalah media pembelajaran dongeng fabel Elang dan Kelinci, dengan persentase kelayakan (1) Penilaian dari ahli media memperoleh presentase 66,6% dengan kategori cukup layak, (2) Penilaian dari ahli materi dan bahasa memperoleh presentase 84,5% dengan kategori layak, (3) Penilaian dari uji angket respons lapangan perseorangan memperoleh presentase 85,5% dengan kategori layak, (4) Penilaian dari uji angket respons lapangan kelompok kecil memperoleh presentase 92,5% dengan kategori sangat layak. Kesimpulan: Media Pembelajaran Dongeng Fabel Elang dan Kelinci Layak digunakan dalam proses pembelajaran pengenalan kemampuan keaksaraan anak usia 5-6 tahun di Wonorejo Kecamatan Glagah Kabupaten Lamongan
INTERFERENSI FONOLOGIS BAHASA TOLAKI DALAM BAHASA INDONESIA SISWA KELAS X SMA NEGERI 1 TONGAUNA
Authors: Ramlin Ramlin
Published: November 2020

Interferensi adalah adanya kemiripan beberapa kata terutama bahasa-bahasa, yang masih serumpun. Seperti halnya suku-¬suku bangsa di Indonesia lainnya, sebagian besar masyarakat suku tolaki di samping menggunakan bahasa daerah sebagai alat komunikasi juga menggunakan bahasa Indonesia. Namun, dalam penggunaan kedua bahasa tersebut ada yang mampu menggunakannya sesuai dengan norma-norma masing-masing bahasa tersebut, tetapi adapula yang kurang mampu, terutama siswa sekolah menengah atas yang belajar bahasa Indonesia sebagai bahasa kedua. Pemakaian bahasa Indonesia oleh siswa tersebut akan dipengaruhi oleh bahasa tolaki, baik sistemnya maupun dalam bentuk bunyinya. Masalah penelitian ini adalah “Interferensi Fonologis Bahasa Tolaki dalam Penggunaan Bahasa Indonesia Siswa Kelas X SMA Negeri 1 Tongauna”. Tujuan penelitian ini untuk memperoleh data dan informasi yang jelas tentang interferensi fonologis bahasa tolaki dalam penggunaan bahasa Indonesia siswa kelas X SMA Negeri 1 Tongauna. Manfaat penelitian ini yaitu dapat memberikan informasi faktual tentang kemampuan menggunakan bahasa Indonesia siswa kelas X SMA Negeri 1 Tongauna, sebagai bahan masukan bagi pengambil kebijakan dalam upaya meningkatkan pembelajaran bahasa Indonesia di SMA dan sebagai bahan informasi bagi peneliti selanjutnya yang sifatnya lebih mendalam dan relevan dengan objek penelitian ini. Jenis Penelitian ini penelitian lapangan karena peneliti terlibat langsung dilapangan atau ke sekolah tempat sampel untuk mengumpulkan data penelitian. Data penelitian ini adalah data lisan yakni berupa tuturan bahasa Tolaki yang disampaikan dalam bercerita oleh siswa kelas X SMA Negeri 1 Tongauna, yang direkam melalui tape recorder. Sumber data penelitian ini adalah semua siswa kelas X SMA Negeri 1 Tongauna sebagai penutur asli yang menggunakan bahasa daerah Tolaki sebagai bahasa ibu atau bahasa pertama. Teknik pengumpulan data yang digunakan dalam penelitian ini adalah teknik observasi, teknik rekam dan teknik wawancara. Hasil penelitian yang diperoleh dari penelitian ini adalah (1) penghilangan fonem, (2) penggantian dan penghilangan fonem, (3) penggantian fonem, (4) interpretasi hasil penelitian.
Sistem Pengendali Suhu Dan Kelembapan Pada Kebun Manggis
Authors: Prasetya Andhis Yoga , Dharmawan Ricky , Muzayyin Ahmad , Rachmatullah Sholeh
Published: November 2020

Tanaman Manggis di daerah Madura memiliki karakteristik tersendiri yaitu suhu udara sekitar yang sangat tinggi  dan kelembapan yang rendah sehingga bisa mempengaruhi pembuahan pada tanaman manggis. Selama ini petani manggis kesulitan untuk menjaga suhu dan kelembapan dalam lahan manggis karena masih menggunakan tenaga manusia untuk menyiramnya sehingga tidak diketahui kapan suhu naik dan turun. Penelitian ini bertujuan untuk membuat aplikasi pendeteksi,mengontrol suhu disekitranya dan kelembapan tanah pada tanaman buah manggis. Merancang perangkat sistem pengontrol suhu dan kelembapan serta penyiraman otomatis di kebun manggis sehingga mengurangi tenaga kerja dalam penyiraman. Pada tahapan pertama akan dirancang perangkat kontrol untuk merekayasa suhu dalam lahan manggis. Adapun   perangkat yang digunakan adalah Arduino Mega 2560, sensor DHT22 , Soil Moisture, Relay, 1 set sistem pengembunan (nozzle Sprayer),pompa kabut. Tahap kedua membuat sistem untuk menampilkan dan mengkontrol kerja dari perangkat tersebut dengan menampilkan pada layar LCD. Hal ini dilakukan agar kerja dari perangkat tersebut bisa memberikan informasi perubahan suhu dan kelembapan. Sistem yang dibangun bisa mendeteksi dan mengontrol suhu dalam lahan manggis serta melakukan pengembunan secara otomatis. Sistem ini dirancang menggunakan teknologi yang open source dan open device sehingga perangkat yang dibuat bisa dikembangkan berdasarkan tujuan penelitian.
APLIKASI DETEKSI WAJAH WAKTU NYATA UNTUK PENGENAL TAMU
Authors: Suyatno Suyatno
Published: November 2020

Perubahan teknologi mendorong manusia untuk lebih efisien dalam melakukan pekerjaan sehari - hari. Perubahan metode kerja dan sumber daya manusia secrara efektif dan efisien yang didukung oleh kondisi lingkungan yang baik dalam memberikan layanan kepada publik. Penelitian  ini bertujuan untuk mendapatkan program aplikasi yang dapat memberikan sapaan kepada tamu(digital recepsionis) berdasrkan obyek yang diterima oleh kamera. Pembuatan Aplikasi deteksi wajah pada pelayanan tamu secara otomatis dilakukan dengan menggunakan program Visual C++ dalam hal ini MFC dan OCx yang dapat mengakses beberapa peralatan yang dihubungkan dengan aplikasi tersebut, pendukung dari alat tersebut adalah kamera web.Perpaduan antara software dan hardware dapat diakses dengan menggunakan program C++. Penerima Tamu secara otomatis menggunakan camera web, akan lebih effisien dalam memberikan pelayanan kepada publik. Hasil yang dicapai pada penelitian ini adalah  dapat diimplementasikan  dalam pelayanan publik di perkantoran, hotel,pertokoan ataupun rumah.
Kajian Literatur Mengenai Klasifikasi Blog
Authors: Husni Husni
Published: November 2020

Klasifikasi blog merupakan topik kajian baru. Teknik klasifikasi web tradisional tidak dapat diterapkan secara langsung terhadap blog karena sering terjadinya update terhadap isi dan variasi topik pada suatu situs blog. Komponen penyusun blog seperti judul, isi dan komentar, tag (label), penulis, hyperlink, permalink, outlink, tanggal dan jam termasuk obyek yang perlu dilibatkan dalam proses klasifikasi. Tulisan ini mencoba meninjau berbagai pendekatan klasifikasi blog yang hadir sejak 2009. Pada awal kemunculan blog, klasifikasi biner digunakan untuk membedakan blog dari halaman web biasa. Kami fokus pada bagaimana mengkategorikan suatu blog ke dalam daftar topik, genre dan opini (mood dan sentimen) yang telah  didefinisikan sebelumnya.Pada klasifikasi topik dan genre, algoritma kNN, Naive Bayes, CFC, SVM  dan pendekatan machine learning lainnnya banyak digunakan.Pemanfaatan ontologi topik dan tag dapat meningkatkan akurasi klasifikasi. Pada deteksi opini, pendekatan berbasis lexicon seperti ANEW cenderung lebih banyak digunakan. Opini dari suatu situs blog juga dapat diprediksi berdasarkan opini di sekitar inlink yang menuju situs tersebut. Kajian ini perlu diperluas dan diperdalam, seperti keterlibatan lebih lanjut dari tag, link dan analisis jejaring sosial.Kata kunci: klasifikasi blog, analisis sentimen, blog mining
Understanding the Dynamic Behavior of Unconventional Horizontal Wells - A Blend of Physics and Data-Based Workflow for the Artificial Lift Life Cycle
Authors: Kannan Subash K , Flowers Kreg , Vangolen Brent
Published: November 2020

The pressure and flow rates in an unconventional horizontal well decline rapidly, making artificial lift (AL) an integral part of development. Due to the dynamic behavior of these wells, the artificial lift life cycle is critical, and detailed attention to the AL system is needed at all stages for optimized recovery. This includes the selection and reselection of the proper AL method, adjusting the operating parameters, and scheduling the related workovers. With higher well counts and every well having some form of AL, a workflow to help in understanding the dynamic nature of the wells and to plan the artificial lift life cycle proactively will be beneficial. This has the potential to add significant value in terms of failure reduction, expense and downtime reductions, and improved operating efficiency. The objective of this paper is to present a workflow using field data and an example case study that is easily scalable and can be applied to any field to add value.
Wheatstone: What We Have Learnt in Early Production Life
Authors: Pescod John , Connell Paul , Xia Zhi , Herriman Justin , Strong Anthony , House Andrew
Published: November 2020

Wheatstone and Iago commenced production in June 2017. Its foundation subsea system includes nine Wheatstone and Iago development wells tied back to a central Wheatstone Platform (WP) for processing. Hydrocarbons then flow via an export pipeline to an onshore processing facility that includes 2 × liquefied-natural-gas (LNG) trains and a domestic gas (Domgas) facility.

Data from subsea wells, manifolds and flowlines, along with data from WP and the Wheatstone LNG Plant are continually monitored to ensure wells and equipment are operating within allowable limits. Observed and expected performance are routinely compared to recognise any unexpected performance for further investigation.

This paper will highlight some of the key learnings in well and reservoir surveillance analysis and optimisation (SA&O) that have been developed using the data from early production. Production strategies will be discussed, along with factors that need to be considered when developing a preferred strategy through operational and production challenges. Insights will be provided in how to assess the performance and reliability of equipment, including pressure and temperature gauges, flowmeters and chokes. Discussion will also include reservoir surveillance and analysis techniques used, what information is yielded from the different analyses and what surveillance and analysis techniques and tools have worked best.

Practical examples will be shared that includes tubing and inflow trends, drawdown analysis, equipment performance, and the operational and production challenges of trunkline pressure management.
Improving maternal and child nutrition in China: An analysis of nutrition policies and programs initiated during the 2000-2015 Millennium Development Goals era and implications for achieving the Sustainable Development Goals
Authors: Huang Xin , Yang Bo , Liu Qin , Zhang Ruilin , Tang Shenglan , Story Mary
Published: November 2020

Background: Although good progress was made in maternal and child nutrition during the Millennium Development Goals (MDGs) era, malnutrition remains one of the major threats on global health. Therefore, the United Nation set several nutrition-related goals in the Sustainable Development Goals (SDGs). There is much to be learned from individual countries in terms of efforts and actions taken to reduce malnutrition. China, as a developing country, launched a number of nutrition improvement policies and programs that resulted in dramatic progress in improving maternal and child nutrition during the MDGs era. This study explored the impact, experiences and lessons learned from the nutrition policies and programs initiated in China during the MDGs era, and implications to achieve the SDGs for China and other developing countries.Method: The CNKI database and official websites of Chinese government were searched for reviews on nutrition-related policies and intervention programs. A qualitative study was conducted among key informants from the Chinese government, Non-governmental Organizations (NGOs) and Universities for two major national nutrition intervention programs. Results: The literature review documented that during the MDGs era six nutrition policies and eight trans-province and nationwide nutrition intervention programs collectively made good progress in improving maternal and child nutrition in China. Nutrition policies tended to be targeted at infants and children, with less attention on reproductive and maternal nutrition. Nutrition intervention programs focused primarily on undernutrition and have achieved positive results, while for breastfeeding improvement and prevention and control on overweight and obesity were limited. Results from the qualitative study indicated that effective nutrition program implementation was facilitated through the cooperation of multiple sectors and by the government and NGOs partnerships, however, still face challenges of insufficient operational funds from local governments, and inadequacy of program monitoring and management. Conclusion: Nutrition policies and intervention programs promulgated in China during the MDGs era have made major contributions to the rapid decline of undernutrition, and are in line to achieve the SDGs related to child wasting, stunting, low birth weight and anemia in reproductive-age women. However, appropriate policies and program implementation are needed to improve exclusive breastfeeding rates and reduce obesity to achieve the SDGs in years to come.
High Performance Alternative Diesel Engine Fuel using Modified Rice Straw Catalyst
Authors: Metwally Rehab , Hashish hassan Abu , El-Samad Haitham Abd , Awad Mostafa , Kadry Ghada
Published: November 2020

Background: The world depends almost on fossil fuels. This leads to depletion of oil and an increase in environmental pollution. Therefore, the researchers search to find alternative fuels. Waste cooking oil (WCO) was selected as feedstock for biodiesel production to eliminates the pollution problems. The agricultural waste is very big and without cost, this leads to the use of the rice straw in preparing a catalyst for biodiesel production. Results: The reusability of the acidic catalyst confirmed that the conversion efficiency was high until after 8 cycles of the production. The highest conversion efficiency of the converting WCO extended to 90.38% with 92.5% maximum mass yield and methyl ester content 97.7% wt. at the optimized conditions. The result was indicating that B15 is the best blend for thermal efficiency and specific fuel consumption. All emission concentrations decrease with increasing the engine load, especially for B15 fuels compared to the diesel oil.Conclusion: The novelty of this paper is assessing the methyl esters from the local WCO as an alternative fuel for diesel engines using a heterogeneous catalyst based on the agricultural waste. The performance of the diesel engines and its exhaust emissions have been experimentally investigated with the produced biodiesel of WCO as a blend (B10, B15, and B20) compared to the diesel.
A case study of the first pregnant women with COVID-19 in Bukavu, Eastern DR Congo
Authors: BIRINDWA ETIENNE KAJIBWAMI , MULUMEODERHWA Guy MULINGANYA , NYAKIO Olivier , MBALE Guy Quesney MATESO , MUSHAMUKA Serge ZIGABE , MATERANYA Jeanne MANINGO , KAHASHA Pierrot MULUMEODERHWA , BISIMWA Yvette KUJIRAKWINJA , KAMPARA Freddy MIRINDI , IRENGE Jules MONGANE , KIBALAMA Isaac BARHISHINDI , LUZADI Pierre KABUYA , MALEMBAKA Espoir BWENGE , MAYERI Daniel GARHALANGWA NA MUNTU , AKONKWA Marius BAGUMA , BALALUKA Ghislain BISIMWA
Published: November 2020

Introduction: Vertical transmission of covid-19 is possible; its risk factors are worth researching. The placental changes found in pregnant women have a definite impact on the foetus.Case presentation: We report a case of a 25-year-old woman, gravida 3, para 2 (2 alive children), with a history of two caesarean deliveries, who was infected by the SARS-Cov-2 during the last term of her pregnancy. She gave birth by caesarean after 34 weeks of gestation to a new-born baby also infected with SARS-Cov-2. The per-operative observations noted several eruptive lesions in the pelvis, bleeding on contact. Microscopic examination of the foetal appendages revealed thrombotic vasculopathy in the placenta and in the umbilical cord vessels.Conclusion: This case is one of the first documented cases of COVID-19 in pregnancy in sub-Saharan Africa. We strongly suggest obstetricians to carefully examine the aspect of the peritoneum, viscera and foetal appendages in affected pregnant women.
Genipin and Insulin Combined Treatment Improves Implant Osseointegration in Type 2 Diabetic Rats
Authors: Zhang Jiajia , Wang Ya-nan , Jia Tingting , Huang Haiyun , Zhang Dongjiao , Xu Xin
Published: November 2020

Background: Type 2 diabetes mellitus (T2DM) has a harmful effect on the stability and osseointegration of dental implants. T2DM induces mitochondrial damage by inhibiting AMPK signaling, resulting in oxidative stress and poor osteogenesis in the peri-implant bone area. Genipin is a major component of gardenia fruits with strong antioxidant, anti-inflammation, and anti-diabetic actions, and it also can activate mitochondrial quality control via the AMPK pathway. The purpose of this study was to investigate the effects of genipin and insulin treatment on implant osseointegration in T2DM rats and explore the underlying mechanisms. Methods: Streptozotocin-induced diabetic rats received implant surgery in their femurs, and were then assigned to five groups that were subjected to different treatments for three months: control group, T2DM group, insulin-treated T2DM group (10 IU/kg), genipin-treated T2DM group (50 mg/kg), and the genipin and insulin combination-treated T2DM group. Then, we regularly assessed the weight and glucose levels of the animals. Rats were euthanized at three months after the implantation procedure, and the femora were harvested for microscopic computerized tomography analysis, biomechanical tests, and different histomorphometric assessment. Results: The results indicated that the highest blood glucose and oxidative stress levels were measured for the T2DM group, resulting in the poorest osseointegration. The combination-treated T2DM group mitigated hyperglycemia and normalized, reactivated AMPK signaling, and alleviated oxidative stress as well as reversed the negative effect of osseointegration. There were beneficial changes observed in the T2DM-genipin and T2DM-insulin groups, but these were less in comparison to the combination treatment group. Conclusion: Our study suggests that treatment with genipin in combination with insulin could be an effective method for promoting implant osseointegration in T2DM rats, which may be related to AMPK signaling.
Drug Resistance of Pathogens Causing Nosocomial Infection in Orthopedics from 2012 to 2017: A 6-Year Retrospective Study
Authors: Yang Xiaowei , Guo Runsheng , Xie Banglin , Lai Qi , Xu Jiaxiang , Hu Niya , Wan Lijun , Dai Min , Zhang Bin
Published: November 2020

Background: Hospital-acquired infections (HAIs) are an emerging global problem that increases in-hospital mortality, length of stay, and cost. We performed a 6-year retrospective study to provide valuable insight into appropriate antibiotic use in HAI cases. We also aimed to understand how hospitals could reduce pathogen drug resistance in a population that overuses antibiotics.Methods: All data (2012–2017) were obtained from the Hospital Information Warehouse and Clinical Microbiology Laboratory.Results: We isolated 1392 pathogen strains from patients admitted to the orthopedics department during 2012–2017. Escherichia coli (14.7%, 204/1392), Enterobacter cloacae (13.9%, 193/1392), and Staphylococcus aureus (11.3%, 157/1392) were the most common pathogens causing nosocomial infections. The dominant Gram-negative bacterium was E. coli, with high resistance to ampicillin, levofloxacin, cotrimoxazole, gentamicin, and ciprofloxacin, in that order. E. coli was least resistant to amikacin, cefoperazone-sulbactam. The most dominant Gram-positive bacterium was S. aureus, highly resistant to penicillin and ampicillin, but not resistant to fluoroquinolones and cotrimoxazole. Analysis of risk factors related to multidrug-resistant bacteria showed that patients with open fractures were significantly more susceptible to methicillin-resistant S. aureus infections (p < 0.05). Additionally, extended-spectrum β-lactamase-producing E. coli infections occurred significantly more often in patients with degenerative diseases (p < 0.05). Elderly patients tended to be more susceptible to multidrug-resistant bacterial infections, but this outcome was not statistically significant.Conclusions:Antimicrobial resistance is a serious problem in orthopedics. To effectively control antimicrobial resistance among pathogens, we advocate extensive and dynamic monitoring of MDR bacteria, coupled with careful use of antibiotics.
Continuous use of glycomacropeptide in the nutritional management of patients with phenylketonuria: a clinical perspective
Authors: Pena Maria João , Pinto Alex , de Almeida Manuela Ferreira , Barbosa Catarina de Sousa , Ramos Paula Cristina , Rocha Sara , Guimas Arlindo , Ribeiro Rosa , Martins Esmeralda , Bandeira Anabela , Dias Cláudia Camila , MacDonald Anita , Borges Nuno , Rocha Júlio César
Published: November 2020

Background: In phenylketonuria (PKU), modified casein glycomacropeptide supplements (CGMP-AA) are used as an alternative to the traditional phenylalanine (Phe)-free L-amino acid supplements (L-AA). However, studies focusing on the long-term nutritional status of CGMP-AA are lacking. This retrospective study evaluated the long-term impact of CGMP-AA over a mean of 29 months in 11 patients with a mean age at CGMP-AA onset of 28 years (range 15 to 43) [8 females; 2 hyperphenylalaninaemia (HPA), 3 mild PKU, 3 classical PKU and 3 late-diagnosed]. Outcome measures included metabolic control, anthropometry, body composition and biochemical parameters. Results: CGMP-AA, providing 66% of protein substitute intake, was associated with no significant change in blood Phe with CGMP-AA compared with baseline (562 ± 289 µmol/L vs 628 ± 317 µmol/L; p=0.065). In contrast, blood tyrosine significantly increased on CGMP-AA (52.0 ± 19.2 μmol/L vs 61.4 ± 23.8 μmol/L; p=0.027). Conclusions: Biochemical nutritional markers remained unchanged which is an encouraging finding in adults with PKU, many of whom are unable to maintain full adherence with nutritionally fortified protein substitutes. Longitudinal, prospective studies with larger sample sizes are necessary to fully understand the metabolic impact of using CGMP-AA in PKU.
Anti-Inflammatory and Tau Phosphorylation–Inhibitory Effects of Eupatin
Authors: Chou Ching-Hsuan , Hsu Kai-Cheng , Lin Tony Eight , Yang Chia-Ron
Published: November 2020

Background: Alzheimer’s disease (AD), which is among the most prevalent neurodegenerative diseases, manifests as increasing memory loss and cognitive decline. Tau phosphorylation and aggregation are strongly linked to neurodegeneration as well as associated with chronic neuroinflammatory processes. The anti-inflammation effects of natural products have led to wide recognition of their potential for use in treating and preventing AD. This study investigated whether eupatin, a polymethoxyflavonoid found in Artemisia species, has inhibitory effects on neuroinflammation and tau phosphorylation. Methods: We treated mouse macrophages and microglia cells with lipopolysaccharides (LPSs) to activate inflammatory signals, and we treated neuronal cells with a protein phosphatase 2A inhibitor, okadaic acid (OA), or transfection with pRK5-EGFP-Tau P301L plasmid to induce tau phosphorylation. Results: The results indicated that eupatin significantly reduced the LPS-induced protein expression and phosphorylation of p65 and inducible nitric oxide synthase as well as downstream products interleukin 6 and nitrite, respectively. Furthermore, eupatin markedly inhibited the expression of phospho-tau in response to OA treatment and plasmid transfection. We discovered that this inhibition was achieved through the inhibition of glycogen synthase kinase 3β (GSK3b), and molecular docking results suggested that eupatin can sufficiently bind to the GSK3b active site. Conclusion: Our results demonstrate that eupatin has neuroprotective effects, making it suitable for AD treatment.
Reflections on Japan’s G20 Presidency in 2019 and The Lessons for Future Political Commitment to Universal Health Coverage
Authors: Sugai Maaya Kita , Ishizuka Aya , Chiba Mina , Iso Hiroyasu , Katsuma Yasushi
Published: November 2020

Background: The Group of 20 (G20) Osaka Summit 2019 was a large step forward for global health diplomacy to build consensus on universal health coverage (UHC). To strengthen multi-stakeholder UHC partnership, Japan involved the research and policy advice network for G20 (Think 20: T20), civil society (Civil 20: C20), private initiatives of medical professional groups (H20), and the pharmaceutical sector. We attempted to identify UHC-related issues addressed and left unaddressed at the G20 Osaka, to bring lessons for the G20 Riyadh Leaders’ Summit 2020. Methods: We reviewed the G20 Osaka Leaders’ Declaration, policy-related statements, and voices of the relevant G20 engagement groups and sectors. In 2019 July, after the G20 Osaka Leaders’ Summit, we organized an expert meeting convening Japan-based UHC-related key global health stakeholders. The main findings were presented in form of classifying the voices expressed in the meeting by UHC-related topics, and then definitional ranges of UHC were summarized.Results: The T20, H20, and the pharmaceutical sector noted during our expert meeting that the ministerial-level health -finance collaboration was one of the key agendas suggested at the G20. T20 and C20 called for a recognition of health needs of refugees, migrants and other vulnerable groups in achieving UHC. Sexual and reproductive health and rights (SRHR) with a human rights-based approach through UHC was raised by the C20 as an issue unaddressed in G20 Osaka. Variation in operative purposes between global health stakeholders led to a definitional difference in the scope of UHC. Discussion: The definitional difference could delay progress of UHC attainment. Addressing migrant and refugee health and SRHR within the context of UHC is further needed. Understanding perspectives of various stakeholders will become increasingly important to well-coordinate multi-actor cooperation with adequate social responsibility and transparency in UHC achievement and public-private partnership. Conclusions: At the G20 Riyadh, for UHC there is need of 1) ensuring an integrated yet comprehensive multi-stakeholder approach towards UHC; 2) incorporating important dimensions such as the marginalized population and gender; and 3) ensuring adequate investments toward health information systems and governance to track health data for the vulnerable population and gender-responsive financing.
mRNP Granule Proteins Fmrp and Dcp1a Differentially Regulate Muscle Stem Cell Quiescence via Reciprocally Regulating mRNP Complexes
Authors: Roy Nainita , Pillai Malini , Patell-Socha Farah , Sundar Swetha , Ganesh Sravya , Aloysius Ajoy , Rumman Mohammed , Gala Hardik Paresh , Hughes Simon Meredith , Zammit Peter , Dhawan Jyotsna
Published: November 2020

Background: During skeletal muscle regeneration, satellite stem cells use distinct pathways to repair damaged myofibers or to self-renew by returning to quiescence. Cellular/mitotic quiescence employs mechanisms that promote a poised or primed state, including altered RNA turnover and translational repression. Here, we investigate the role of mRNP granule proteins Fragile X Mental Retardation Protein (Fmrp) and Decapping protein 1a (Dcp1a) in muscle stem cell quiescence and differentiation.Methods: Using isolated single muscle fibers from adult mice, we established differential enrichment of mRNP granule proteins including Fmrp and Dcp1a in muscle stem cells vs. myofibers. We investigated muscle tissue homeostasis in adult Fmr1-/- mice, analyzing myofiber cross-sectional area in vivo and satellite cell proliferation ex vivo. We explored the molecular mechanisms of Dcp1a and Fmrp function in quiescence, proliferation and differentiation in a C2C12 culture model. Here, we used polysome profiling, imaging and RNA/protein expression analysis to establish the abundance and assembly status of mRNP granule proteins in different cellular states, and the phenotype of knockdown cells.Results: Quiescent muscle satellite cells are enriched for puncta containing the translational repressor Fmrp, but not the mRNA decay factor Dcp1a. MuSC isolated from Fmr1-/- mice exhibit defective proliferation and mature myofibers show reduced cross-sectional area, suggesting a role for Fmrp in muscle homeostasis. Expression and organization of Fmrp and Dcp1a varies between different cell states in culture. Consistent with its role as a translational repressor, Fmrp is enriched in non-translating mRNP complexes abundant in quiescent myoblasts; Dcp1a puncta are lost in quiescence, suggesting stabilized and repressed transcripts. The function of each protein differs during proliferation; whereas Fmrp knockdown led to decreased proliferation and lower cyclin expression, Dcp1a knockdown led to increased cell proliferation and higher cyclin expression. However, knockdown of either Fmrp or Dcp1a led to compromised differentiation. We also observed cross-regulation of decay versus storage mRNP granules; knockdown of Fmrp enhances accumulation of Dcp1a puncta, whereas knockdown of Dcp1a leads to increased Fmrp in puncta.Conclusions: Taken together, our results provide evidence that the balance of mRNA turnover versus utilization is specific for distinct cellular states.
Real Deposit Rate and Credit Supply Nexus in ECOWAS Evidence From DCCE-MG and PMG Techniques
Authors: ADELEYE Bosede Ngozi , ADUSEI Michael , OLOHUNLANA Olayinka , AKINYEMI-BABAJIDE Opeyemi , SANAKARAN Arumugam
Published: November 2020

This study interrogates the McKinnon-Shaw hypothesis that artificial depression of the real deposit interest rate depresses the supply of credit by banks. The sample coverage from 1980 to 2015 consists of ten members of the Economic Community of West African States made up of Communauté Financière d'Afrique (CFA) and non-CFA countries. This two groups have very different monetary structures. Engaging the dynamic common correlated effects-mean group (DCCE-MG) and pooled mean group (PMG) techniques, findings validate the hypothesis as the real deposit rate exhibits a positive and long-run impact on credit supply. Monotonic impact is not sustained. These outcomes are corroborated with robustness checks from the respective sub-samples though country-level results are mixed. Similarly, causality test shows that the real deposit rate Granger-causes credit supply in the long-run. Overall, the findings support the McKinnon-Shaw hypothesis that interest rate is an essential ingredient in the intermediation role of the financial system.
Intraoperative Indocyanine Green Video Angiography (ICG–VA) with FLOW 800 Software in Complex Intracranial Aneurysm Surgery
Authors: Xue Tao , Deng Ruming , Gao Bixi , Wang Zilan , Ma Chao , You Wanchun , Zhu Yun , Chen Zhouqing , Wang Zhong
Published: November 2020

Objective: Indocyanine green video angiography (ICG–VA) is a safe and effective instrument to assess changes in cerebral blood flow during cerebrovascular surgery. After ICG-VA, FLOW 800 provides a color-coded map to directly observe the dynamic distribution of blood flow and to calculate semiquantitative blood flow parameters later. The purpose of our study is to assess whether FLOW 800 is useful for surgery of complex intracranial aneurysms and to provide reliable evidence for intraoperative decision making.Methods: We retrospectively reviewed patients with complex aneurysms that underwent microsurgical and intraoperative evaluation of ICG-VA and FLOW 800 color-coded maps from February 2019 to May 2020. FLOW 800 data were correlated with patient characteristics, clinical outcomes, and intraoperative decision-making.Results: The study included 32 patients with 42 complex aneurysms. All patients underwent ICG-VA FLOW 800 data provided semiquantitative data regarding localization, flow status in major feeding arteries; color maps confirmed relative adequate flow in parent, branching and bypass vessels. Conclusions: FLOW 800 is a useful supplement to ICG-VA for intraoperative cerebral blood flow assessment. ICG-VA and FLOW 800 can help to determine the blood flow status of the parent artery after aneurysm clipping and the bypass vessels after aneurysm bypass surgery.
Prevalence And Factors Associated With Depression Among Adults With Sickle Cell Disease at Mulago Hospital, Uganda: a Cross Sectional Study
Authors: Mubangizi Ivan , Nakimuli-Mpungu Etheldreda , Kawooya Ismael , Sekaggya-wiltshire Christine
Published: November 2020

BACKGROUND

Depression among patients with sickle cell disease (SCD) is under diagnosed and undertreated due to the overlap of symptoms and signs of SCD and depression. The study sought to determine the prevalence and factors associated with depression among adults attending the SCD out-patient clinic in Mulago Hospital, Uganda.
METHODS

This was a cross sectional study in which 255 adults with SCD were enrolled. Participants were evaluated for depression using the Self Report Questionnaire (SRQ-20) and a score of 6 was considered diagnostic of depression. Demographic data was collected with a pre-tested study questionnaire. Perceived social support was measured using the 12-item multidimensional social support scale and, self-esteem was measured using the Rosenberg Self-Esteem Scale. Blood samples were taken to obtain a complete blood count. Modified poisson regression analyses were used to determine associations of depression.
RESULTS

The prevalence of depression was 68.2% (95% C.I; 62–74) with a median age of 21 years. The factors independently associated with depression were pain crisis in the last month (prevalence ratio (PR) = 1.07, 95% CI: 1.04–1.07, p = 0.001), history of a hospital admission in the past 6 months (PR = 1.04, 95% CI: 1.01–1.07, p = 0.012), formal education (PR = 0.79, 95% CI: 0.59–0.97, P = 0.008) and a low social support rating (PR = 0.67, 95% CI: 0.53–0.84, P = 0.0019)
CONCLUSIONS

The prevalence of depression in adults with SCD is high with up to two thirds of patients having some form of depression. The major risk factors were low level of education, low social support, pain crises in the past month and hospital admissions in the last 6 months.
Feasibility of Delivering Parent-Implemented NDBI Interventions in Low Resource Regions: A Pilot Randomized Controlled Study
Authors: Rogers Sally J , Stahmer Aubyn , Talbott Meagan , Young Gregory , Fuller Elizabeth , Pellecchia Melanie , Barber Angela , Griffith Elizabeth
Published: November 2020

Background: This implementation feasibility study was conducted to determine whether an evidence-based parent -implemented distance-learning intervention model for young children at high likelihood of having ASD could be implemented at fidelity by Part C community providers and by parents in low resource communities.Methods: The study used a community academic partnership model to adapt an evidence-based intervention tested in the current pilot trial involving randomization by agency in four states and enrollment of 35 coaches and 34 parent-family dyads. After baseline data were gathered, providers in the experimental group received up to 12 hours of training while control providers received six webinars on early development. Six months of intervention with children-families then followed, concluding with data collection. Regression analyses were used to model outcomes of the coach behaviors, the parent fidelity ratings, and child outcomes.Results: A block design model-building approach was used to test the null model followed by the inclusion of group as a predictor, and finally the inclusion of the planned covariates. Model fit was examined using changes in R2 and F-statistic. As hypothesized, results demonstrated significant gains in (1) experimental provider fidelity of implementation compared to the control group; and (2) experimental parent fidelity of implementation compared to the control group. There were no significant differences between groups in child developmental scores. Conclusions: Even though the experimental parent group averaged less than 30 minutes weekly with providers in the 6 months, both providers and parents demonstrated statistically significant gains with moderate effect sizes compared to control groups. Since child changes in parent mediated models are dependent upon the parents’ ability to deliver the intervention, and since parent delivery is dependent upon providers who are coaching the parents, these results demonstrated that two of these three links of the chain were positively affected by the experimental implementation model. However, lack of significant differences in child group gains suggests that further work is needed on this model. Factors to consider include amount of contact with the provider, amount of practice children experience, amount of contact both providers and parents spend on training materials, and motivational strategies for parents, among others. Trial registration: Registry of Efficacy and Effectiveness Studies: #4360, registered 1xx, October, 2020 – Retrospectively registered, http://
Efficient CSP Model Checking for Symbolic State-space Exploration with Application in Bacterial Chemo-Taxis Modelings
Authors: GUO Jing
Published: November 2020

Event locality is a class of theory that can clearly minimizes the memory and time required to store the state space. We present the multi-way decision diagrams to encode the next-state functions based on event locality and CSP’s stable failure. Instead of studying symbolic overall model, we apply the above thinking to the set of sub-models. Furthermore, we focus on saturation algorithms for CSP theory. And the algorithms consist of two phases, the universal process’s saturation checking and the single CSP symbol’s saturation checking. In the former case, the algorithms discuss better iteration strategy and recursive local fifix-point computations. In the latter case, the the single CSP symbol’s saturation checking calls the former algorithms, being combined preformed events and refusal events. Finally, we discuss bacterial chemotaxis modeled in CSP’s framework and saturation checking used to check the predefifined properties. The all algorithms are implemented in CSP tool FDR, the running results show that our algorithms often perform signifificantly faster than other conventional algorithms.
MiR-365-3p is Involved in IL17-Mediated Asthma in Mouse Model
Authors: Wang Weijia , Li Ying , Qu Xiaoyan , Shang Dong , Qin Qiaohong , Hamid Qutayba , Dang Xiaomin , Chang Ying
Published: November 2020

BACKGROUND The IL-17 superfamily, which mediates cross-talk between the adaptive and innate immune systems, has been associated with severity of asthma. The role of miRNAs in the disease has been paid much attention. To explore the roles of IL-17 in asthma and the relationship between IL-17 and miRNAs, we used a model of severe asthma driven by chronic respiratory exposure to house dust mite (HDM) exposure in wild type and IL-17KO mice, followed with miRNA profiling assays and analysis.METHODS Male and female C57BL/6 mice (6-8 weeks old) and IL-17KO mice (C57BL/6 background) were exposed to purified HDM extract intranasally for 5 days/week for 5 consecutive weeks. Sterile saline was used as the control. The parameters including airway responsiveness, inflammatory cells in bronchoalveolar lavage fluid (BALF), airway smooth muscle bundle, collagen deposition, and cytokine levels in BALF were examined. The miRNA profile of mouse lung tissue was analyzed by microarray assays. The dysregulation of miRNA related to IL-17 and asthma was validated by qRT-PCR. The in vitro cell culture experiment was performed to confirm the relationship between IL-17 and selected miRNA. The regulation of miRNA on predicted target gene was validated by administration of miRNA mimics. RESULTS The expression of IL-17A significantly increased in wild type (WT) mice with HDM exposure compared to the control mice. IL-17 deficiency did not reduce airway hyper responsiveness (AHR) induced by HDM exposure. In comparison to HDM-exposed WT mice, BALF neutrophils in IL-17KO mice were significantly decreased. In WT mice, HDM exposure led to increased expression of IL-4 and KC, which was significantly decreased in IL-17KO mice. Furthermore, under HDM exposure, significantly less airway smooth muscle mass and collagen deposition was found in IL-17KO mice compared to WT mice. In the dysregulated miRNAs, the decreased expression of miR-365-3p in HDM-exposed WT mice was validated, and its expression recovered in IL-17KO mice. Furthermore, miR-365-3p was decreased in mouse alveolar epithelial cells by IL-17 treatment. The transfection of miR-365-3p mimics decreased the expression of predicted target gene ARRB2.