bluuebunny/crossref_metadata_2025 huggingface dataset:
Linguistic analysis of human-computer interaction
Authors: Zellou Georgia , Holliday Nicole
Published: May 2024

This article reviews recent literature investigating speech variation in production and comprehension during spoken language communication between humans and devices. Human speech patterns toward voice-AI presents a test to our scientific understanding about speech communication and language use. First, work exploring how human-AI interactions are similar to, or different from, human-human interactions in the realm of speech variation is reviewed. In particular, we focus on studies examining how users adapt their speech when resolving linguistic misunderstandings by computers and when accommodating their speech toward devices. Next, we consider work that investigates how top-down factors in the interaction can influence users’ linguistic interpretations of speech produced by technological agents and how the ways in which speech is generated (via text-to-speech synthesis, TTS) and recognized (using automatic speech recognition technology, ASR) has an effect on communication. Throughout this review, we aim to bridge both HCI frameworks and theoretical linguistic models accounting for variation in human speech. We also highlight findings in this growing area that can provide insight to the cognitive and social representations underlying linguistic communication more broadly. Additionally, we touch on the implications of this line of work for addressing major societal issues in speech technology.
Inflammation and coronary microvascular disease: relationship, mechanism and treatment
Authors: Guo Zehui , Yang Zhihua , Song Zhihui , Li Zhenzhen , Xiao Yang , Zhang Yuhang , Wen Tao , Pan Guiyun , Xu Haowei , Sheng Xiaodi , Jiang Guowang , Guo Liping , Wang Yi
Published: May 2024

Coronary microvascular disease (CMVD) is common in patients with cardiovascular risk factors and is linked to an elevated risk of adverse cardiovascular events. Although modern medicine has made significant strides in researching CMVD, we still lack a comprehensive understanding of its pathophysiological mechanisms due to its complex and somewhat cryptic etiology. This greatly impedes the clinical diagnosis and treatment of CMVD. The primary pathological mechanisms of CMVD are structural abnormalities and/or dysfunction of coronary microvascular endothelial cells. The development of CMVD may also involve a variety of inflammatory factors through the endothelial cell injury pathway. This paper first reviews the correlation between the inflammatory response and CMVD, then summarizes the possible mechanisms of inflammatory response in CMVD, and finally categorizes the drugs used to treat CMVD based on their effect on the inflammatory response. We hope that this paper draws attention to CMVD and provides novel ideas for potential therapeutic strategies based on the inflammatory response.
Circulating metabolites and coronary heart disease: a bidirectional Mendelian randomization
Authors: Chen Huanyu , Huang Yuxuan , Wan Guangjing , Zou Xu
Published: May 2024

Background

Numerous studies have established a link between coronary heart disease and metabolic disorders. Yet, causal evidence connecting metabolites and Coronary Heart Disease (CHD) remains scarce. To address this, we performed a bidirectional Mendelian Randomization (MR) analysis investigating the causal relationship between blood metabolites and CHD.

Methods

Data were extracted from published genome-wide association studies (GWASs) on metabolite levels, focusing on 1,400 metabolite summary data as exposure measures. Primary analyses utilized the GWAS catalog database GCST90199698 (60,801 cases and 123,504 controls) and the FinnGen cohort (43,518 cases and 333,759 controls). The primary method used for causality analysis was random inverse variance weighting (IVW). Supplementary analyses included MR-Egger, weighted mode, and weighted median methods. Sensitivity analyses were conducted to evaluate heterogeneity and pleiotropy. Reverse MR analysis was employed to evaluate the direct impact of metabolites on coronary heart disease. Additionally, replication and meta-analysis were performed. We further conducted the Steiger test and colocalization analysis to reflect the causality deeply.

Results

This study identified eight metabolites associated with lipids, amino acids and metabolite ratios that may influence CHD risk. Findings include: 1-oleoyl-2-arachidonoyl-GPE (18:1/20:4) levels: OR = 1.08; 95% CI 1.04–1.12; P = 8.21E-06; 1-palmitoyl-2-arachidonoyl-GPE (16:0/20:4) levels: OR = 1.07; 95% CI 1.04–1.11; P = 9.01E-05; Linoleoyl-arachidonoyl-glycerol (18:2/20:4): OR = 1.08; 95% CI 1.04–1.22; P = 0.0001; Glycocholenate sulfate: OR = 0.93; 95% CI 0.90–0.97; P = 0.0002; 1-stearoyl-2-arachidonoyl-GPE (OR = 1.07; 95% CI 1.03–1.11; P = 0.0002); N-acetylasparagine (OR = 1.04; 95% CI 1.02–1.07; P = 0.0030); Octadecenedioate (C18:1-DC) (OR = 0.93; 95% CI 0.90–0.97; P = 0.0004); Phosphate to linoleoyl-arachidonoyl-glycerol (18:2–20:4) (1) ratio (OR = 0.92; 95% CI 0.88–0.97; P = 0.0005).

Conclusion

The integration of genomics and metabolomics offers novel insights into the pathogenesis of CHD and holds significant importance for the screening and prevention of CHD.
A landscape index for indicating the spatio-temporal dynamics of carbon storage in an opencast coal mine
Authors: Chang Ming , Meng Shuying , He Xinran , Chen Long , Zhao Lei , Yang Haitao , Wang Ruiguo , Wang Xianghao , Zhao Yuxia , Zhao Peng
Published: May 2024

Coal is China’s main resource, with open-pit mining accounting for a significant portion of global production. However, this activity, including mining and ecological restoration, can have a definite impact on ecosystem carbon storage and its distribution; its associated factors are also unclear. In this paper, we quantify the carbon storage changes in Haerwusu coal mine, a typical large-scale coal mine in China, based on land use/land cover (LULC) characteristics, and analyze the impact factors of carbon density from 2007 to 2022 by integrating the InVEST model with the landscape ecological function contribution ratio and multiple regression model. The results are as follows. (1) Carbon storage decreased from 159.95 × 104 to 147.51 × 104 from 2007 to 2017 and then increased to 151.91 × 104 to 2022. (2) The degree of coordination between carbon storage forest and grassland area landscape pattern coupling ranged from 0.887 to 0.867 from 2007 to 2022, with the lowest point at 0.720 in 2012. (3) Carbon storage was significantly related to vegetation indices, temperature, and elevation, and these factors can explain 37.5% of the carbon storage spatial variability; stepwise regression analysis showed that the integration of landscape patterns, such as Shannon’s diversity index (SHEI) and the aggregation index (AI), could improve the explanation by 1.4%. (4) Based on the analysis of the landscape ecological function contribution ratio, the carbon storage-sensitive areas can be classified into three levels: extremely sensitive areas ranging 0 to 4 km from the mine, sensitive areas ranging 4 to 8 km, and insensitive areas ranging beyond 8 km. This study proposes a strategy for analyzing changes of carbon storage in coal mines, highlights the important role of landscape patterns in influencing carbon storage, and provides a reliable reference support for the ecological management of coal mines.
Consolidation effects on pipe-soil interaction due to tunneling
Authors: Liang Xu , Xu Jian , Huang Yong , Shi Li , Zeng Zhiquan , Miao Baozhu , Huang Lei , Wu Jian
Published: May 2024

Existing studies on soil-pipe interaction due to tunneling mainly focus on short-term responses. However, in areas with high water tables and low permeability soil, long-term ground movement and associated pipe responses may occur due to dissipation of excess pore pressure generated during tunnel construction. In this paper, a Winkler solution with time-varying subgrade modulus and the corresponding greenfield soil displacement formula are developed to investigate the tunneling effects on existing pipelines. The pipe is considered as an infinite Euler beam of finite width resting on a poroelastic half-space, and adhesion and drainage effects between the pipe and soil are considered using bounding techniques. The greenfield consolidation settlement is evaluated using a modified Gaussian curve. The findings indicate that the subgrade modulus decreases while greenfield soil displacement increases during the consolidation process. The time-dependent behavior of the subgrade modulus is governed by the drainage condition at the pipe-soil interface, whereas the greenfield soil displacement is primarily influenced by the drainage condition at the tunnel-soil interface. The study reveals that the bonded contact condition, hydraulic boundary condition, and displacement constraint conditions all influence the bending moment of the pipe.
Unveiling the black box: exploring teachers’ approaches to ethnic victimization incidents at school
Authors: Bayram Özdemir Sevgi , Özdemir Metin
Published: May 2024

Schools are crucial socialization contexts where civic norms and values such as appreciating diverse perspectives and embracing differences can be systematically transmitted to the next generations. This process, in turn, can foster the development of more inclusive societies. However, increasing polarized political climate poses a risk for the formation of harmonious interactions between youth of different ethnic origins in schools. Teachers are considered as crucial resources in addressing negative student interactions and helping victims in overcoming the consequences of their negative experiences. Nevertheless, our understanding of how teachers respond to ethnic victimization incidents is limited, along with the factors influencing their responses. To address this gap in knowledge, we examined the relative contributions of teachers’ general efficacy (i.e., managing disruptive behaviors in class) and diversity-related efficacy (i.e., addressing challenges of diversity) on their responses to ethnic victimization incidents. The sample consisted of head teachers of 8th grade students (N = 72; 56% females). The results showed that teachers adopt a diverse range of strategies to address incidents of ethnic victimization, with a primary focus on prioritizing the comfort of the victim as the foremost action. Further, we found that teachers’ efficacy in handling disruptive behaviors in class, as opposed to their efficacy in addressing diversity-related issues, explained their responses to victimization incidents. Specifically, teachers with a high sense of efficacy for classroom management were more likely to contact parents of both victims and perpetrators and to provide comfort to the victim. These findings highlight the necessity of supporting teachers to enhance their efficacy in classroom management, and in turn to address potential challenges in diverse school settings more effectively.
The impact of advanced practitioners on patients in acute care—A mini review
Authors: Bauernfeind Lydia , Fels Milena , Dahlmann Philipp , Rester Christian , Sterr Fritz
Published: May 2024

Background

Acute patient care is becoming increasingly complex due to various factors (e.g., demographic changes, multimorbidity, organizational changes). Educating healthcare professionals as advanced practitioners at Master's level is one response to these challenges. Meanwhile, there are several empirical insights into advanced roles.

Aim

To provide an overview on the impact of advanced practitioners on acute care patients' outcomes and safety.

Methods

We conducted a mini-review. Therefore, a systematic literature search was carried out in the four databases Medline, CINAHL, Cochrane Library, and PEDro. After four researchers screened the results and extracted the data independently, the included studies were analyzed thematically and clustered along patients' outcomes. The results are presented narratively.

Results

4,332 records were identified in the systematic search, and 67 articles were included in our review. Of these, the majority (n = 57) addressed the nursing profession. Advanced practitioners demonstrated an overall positive impact on acute care patients. They improved patients' vital signs, and reduced their mortality and adverse events (e.g., thrombosis, pneumonia, pressure injury). In addition, they shortened patients' length of stay and waiting times, and reduced admission rates. Patient satisfaction also increased after their implementation. When being compared to physicians, advanced practitioners in acute care demonstrated similar to equal patient outcomes and safety.

Conclusion

The identified studies highlight the benefits of advanced practitioners for acute care patients. While most studies were conducted in emergency departments or intensive care units, further research concerning other acute settings (e.g., psychiatry) or clinical conditions (e.g., cognitive impairment) is recommended.
Efficacy and safety of advanced hybrid closed loop systems in children with type 1 diabetes younger than 6 years
Authors: Rapini Novella , Martino Mariangela , Arnaldi Claudia , Deodati Annalisa , Anagnostopoulou Lilian , Amodeo Maria Elisa , Ciampalini Paolo , Pampanini Valentina , Lorubbio Antonella , Tosini Davide , Cianfarani Stefano , Schiaffini Riccardo
Published: May 2024

Background

Tight glycemic control is essential for the normal growth and development of preschool children. The aim of our study was to evaluate the impact of advanced hybrid closed loop (AHCL) systems in a real-life setting in children younger than 6 years.

Methods

We conducted a two-center prospective study. We enrolled 19 patients with a median age at disease onset of 2.6 years [interquartile range (IQR) 1.6; 4.4] and a median disease duration of 1.4 years (IQR 0.9; 2.8) who were switched to AHCL from multiple daily injections or open-loop insulin therapy and with a 6-month follow-up. Clinical data, sensor glycemic metrics, and pump settings were collected and analyzed.

Results

After 6 months of follow-up, there was a significant reduction in median HbA1c (p = 0.0007) and glucose management indicator (p = 0.03). A reduction in both mild (>180 mg/dL) (p = 0.04) and severe (>250 mg/dL) (p = 0.01) hyperglycemia was observed after 1 month of auto mode, and in mild hyperglycemia, it persisted up to 6 months (p = 0.02). A small increase in time below range (<70 mg/dL) was observed (p = 0.04) without a significant difference in time <54 mg/dL (p = 0.73). Time in range increased significantly, reaching a 10% increment (p = 0.03) compared with baseline. A significant reduction in the average sensor glucose was observed (p = 0.01) while coefficient of glucose variability (CV%) remained stable (p = 0.12). No episodes of ketoacidosis or severe hypoglycemia have been recorded.

Conclusion

AHCL systems are effective and safe for children younger than 6 years and should be considered as a valid therapeutic option from diabetes onset.
Plant diversity in the understory of Eucalyptus plantations on Hainan Island and its response to environmental factors
Authors: Chen Haihui , Du Shan , Huang Hai , Tian Leyu , Zhou Hua , Wu Jinqun , Yu Xuebiao
Published: May 2024

Research on understory plant diversity and its response to environmental factors helps in the sustainable development of plantation forests. We investigated the characteristics of understory plant diversity in Eucalyptus plantation forests located in Dongfang, Ding'an, Tunchang, and Lingao on Hainan Island by leveraging the plot survey method, and analyzing how the understory plant diversity in these Eucalyptus plantation forests responds to environmental factors. The results showed that a total of 124 plant species belonging to 62 families and 112 genera were recorded in the sampled plots of the Dongfang, Ding’an, Tunchang, and Lingao regional sites on Hainan Island, among which species of Fabaceae and Poaceae comprised the largest number of plants. The number of species and plant diversity indices of the shrub layer and herb layer in Eucalyptus plantation forests varied at different sites, The richest understory vegetation in Tunchang, located in the center of Hainan Island, and the highest α-diversity whether gauged by species or phylogenetically. The similarity of the understory plant community species was greatest between Ding’an and Tunchang, whereas the difference in composition was largest between Dongfang and the other three sites. Phylogenetically, the understory plant community at Ding’an had the most distant affinities among species, whereas that at Tunchang had the closest affinities among species. The results of the Mantel test and redundancy analysis revealed differing correlations between plant diversity in the shrub layer versus herb layer and various environmental factors. In particular, elevation and annual average temperature are the two main factors influencing plant diversity in the shrub layer, and soil available nitrogen and annual average sunshine duration are the two main factors influencing plant diversity in the herb layer. Variance decomposition showed that the combined effect of soil, climate, and topography factors is the main driver shaping plant diversity in the shrub layer of the understory in Eucalyptus plantation forests, while the combined effect of climate and soil factors is the main one determining plant diversity in their herb layer.
Biologics targeting IL-17 sharply reduce circulating T follicular helper and T peripheral helper cell sub-populations in psoriasis
Authors: Tsiogkas Sotirios G. , Mavropoulos Athanasios , Dardiotis Efthimios , Zafiriou Efterpi , Bogdanos Dimitrios P.
Published: May 2024

Introduction

Circulating T follicular helper (cTfh) cells and circulating T peripheral helper (cTph) cells (which share common characteristics with the cTfh population) are implicated in the pathogenesis of immune-mediated and autoimmune diseases such as psoriasis (Ps). Their close interplay with the interleukin 17 (IL-17) axis and the ex vivo effect of IL-17-targeting biologic agents used to treat Ps on them are elusive. This study aimed to investigate the effect of biologics targeting IL-17 on cTfh and cTph cell subpopulations isolated from the blood of patients with Ps.

Methods

Peripheral blood mononuclear cells (PBMCs) were isolated from patients with Ps at treatment initiation and three months later. Samples were also collected from controls. Cells were stained using monoclonal antibodies. Flow cytometry assessed the fraction of cTfh (CD3+CD4+CXCR5+) and cTph (CD3+CD4+CXCR5-PD-1hi) cells..

Results

Flow cytometric analysis showed increased fractions of activated cTfh subsets including ICOS+ and ICOS+PD-1+ expressing cells, in patients compared to controls. Biologic blocking of IL-17A diminished the cTfh population. Furthermore, ICOS+ and ICOS+PD-1+ sub-populations were also inhibited. Finally, the cTph cell fraction significantly decreased after three months of successful treatment with biologics.

Conclusion

Early anti-IL-17-mediated clinical remission in Ps is associated with decreased cTfh and cTph cell subpopulations.
Sex-related immunity: could Toll-like receptors be the answer in acute inflammatory response?
Authors: Popotas Alexandros , Casimir Georges Jacques , Corazza Francis , Lefèvre Nicolas
Published: May 2024

An increasing number of studies have highlighted the existence of a sex-specific immune response, wherein men experience a worse prognosis in cases of acute inflammatory diseases. Initially, this sex-dependent inflammatory response was attributed to the influence of sex hormones. However, a growing body of evidence has shifted the focus toward the influence of chromosomes rather than sex hormones in shaping these inflammatory sex disparities. Notably, certain pattern recognition receptors, such as Toll-like receptors (TLRs), and their associated immune pathways have been implicated in driving the sex-specific immune response. These receptors are encoded by genes located on the X chromosome. TLRs are pivotal components of the innate immune system, playing crucial roles in responding to infectious diseases, including bacterial and viral pathogens, as well as trauma-related conditions. Importantly, the TLR-mediated inflammatory responses, as indicated by the production of specific proteins and cytokines, exhibit discernible sex-dependent patterns. In this review, we delve into the subject of sex bias in TLR activation and explore its clinical implications relatively to both the X chromosome and the hormonal environment. The overarching objective is to enhance our understanding of the fundamental mechanisms underlying these sex differences.
Transcriptome profiling of immune rejection mechanisms in a porcine vascularized composite allotransplantation model
Authors: Zhang Lei , Arenas Hoyos Isabel , Helmer Anja , Banz Yara , Zubler Cédric , Lese Ioana , Hirsiger Stefanie , Constantinescu Mihai , Rieben Robert , Gultom Mitra , Olariu Radu
Published: May 2024

Background

Vascularized composite allotransplantation (VCA) offers the potential for a biological, functional reconstruction in individuals with limb loss or facial disfigurement. Yet, it faces substantial challenges due to heightened immune rejection rates compared to solid organ transplants. A deep understanding of the genetic and immunological drivers of VCA rejection is essential to improve VCA outcomes

Methods

Heterotopic porcine hindlimb VCA models were established and followed until reaching the endpoint. Skin and muscle samples were obtained from VCA transplant recipient pigs for histological assessments and RNA sequencing analysis. The rejection groups included recipients with moderate pathological rejection, treated locally with tacrolimus encapsulated in triglycerol-monostearate gel (TGMS-TAC), as well as recipients with severe end-stage rejection presenting evident necrosis. Healthy donor tissue served as controls. Bioinformatics analysis, immunofluorescence, and electron microscopy were utilized to examine gene expression patterns and the expression of immune response markers.

Results

Our comprehensive analyses encompassed differentially expressed genes, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathways, spanning various composite tissues including skin and muscle, in comparison to the healthy control group. The analysis revealed a consistency and reproducibility in alignment with the pathological rejection grading. Genes and pathways associated with innate immunity, notably pattern recognition receptors (PRRs), damage-associated molecular patterns (DAMPs), and antigen processing and presentation pathways, exhibited upregulation in the VCA rejection groups compared to the healthy controls. Our investigation identified significant shifts in gene expression related to cytokines, chemokines, complement pathways, and diverse immune cell types, with CD8 T cells and macrophages notably enriched in the VCA rejection tissues. Mechanisms of cell death, such as apoptosis, necroptosis and ferroptosis were observed and coexisted in rejected tissues.

Conclusion

Our study provides insights into the genetic profile of tissue rejection in the porcine VCA model. We comprehensively analyze the molecular landscape of immune rejection mechanisms, from innate immunity activation to critical stages such as antigen recognition, cytotoxic rejection, and cell death. This research advances our understanding of graft rejection mechanisms and offers potential for improving diagnostic and therapeutic strategies to enhance the long-term success of VCA.
Recent advances in molecular mechanisms of skin wound healing and its treatments
Authors: Mamun Abdullah Al , Shao Chuxiao , Geng Peiwu , Wang Shuanghu , Xiao Jian
Published: May 2024

The skin, being a multifaceted organ, performs a pivotal function in the complicated wound-healing procedure, which encompasses the triggering of several cellular entities and signaling cascades. Aberrations in the typical healing process of wounds may result in atypical scar development and the establishment of a persistent condition, rendering patients more vulnerable to infections. Chronic burns and wounds have a detrimental effect on the overall quality of life of patients, resulting in higher levels of physical discomfort and socio-economic complexities. The occurrence and frequency of prolonged wounds are on the rise as a result of aging people, hence contributing to escalated expenditures within the healthcare system. The clinical evaluation and treatment of chronic wounds continue to pose challenges despite the advancement of different therapeutic approaches. This is mainly owing to the prolonged treatment duration and intricate processes involved in wound healing. Many conventional methods, such as the administration of growth factors, the use of wound dressings, and the application of skin grafts, are used to ease the process of wound healing across diverse wound types. Nevertheless, these therapeutic approaches may only be practical for some wounds, highlighting the need to advance alternative treatment modalities. Novel wound care technologies, such as nanotherapeutics, stem cell treatment, and 3D bioprinting, aim to improve therapeutic efficacy, prioritize skin regeneration, and minimize adverse effects. This review provides an updated overview of recent advancements in chronic wound healing and therapeutic management using innovative approaches.
The predictive value of TNF family for pulmonary tuberculosis: a pooled causal effect analysis of multiple datasets
Authors: Mo Wenxiu , Cui Zhezhe , Zhao Jingming , Xian Xiaomin , Huang Minying , Liu Jun
Published: May 2024

Objective

Despite extensive research on the relationship between pulmonary tuberculosis (PTB) and inflammatory factors, more robust causal evidence has yet to emerge. Therefore, this study aims to screen for inflammatory proteins that may contribute to the susceptibility to PTB in different populations and to explain the diversity of inflammatory and immune mechanisms of PTB in different ethnicity.

Methods

The inverse variance weighted (IVW) model of a two-sample Mendelian Randomization (MR) study was employed to conduct causal analysis on data from a genome-wide association study (GWAS). This cohort consisting PTB GWAS datasets from two European and two East Asian populations, as well as 91 human inflammatory proteins collected from 14,824 participants. Colocalization analysis aimed to determine whether the input inflammatory protein and PTB shared the same causal single nucleotide polymorphisms (SNPs) variation within the fixed region, thereby enhancing the robustness of the MR Analysis. Meta-analyses were utilized to evaluate the combined causal effects among different datasets.

Results

In this study, we observed a significant negative correlation between tumor necrosis factor-beta levels (The alternative we employ is Lymphotoxin-alpha, commonly referred to as LT) (P < 0.05) and tumor necrosis factor receptor superfamily member 9 levels (TNFRSF9) (P < 0.05). These two inflammatory proteins were crucial protective factors against PTB. Additionally, there was a significant positive correlation found between interleukin-20 receptor subunit alpha levels (IL20Ra) (P < 0.05), which may elevate the risk of PTB. Colocalization analysis revealed that there was no overlap in the causal variation between LT and PTB SNPs. A meta-analysis further confirmed the significant combined effect of LT, TNFRSF9, and IL20Ra in East Asian populations (P < 0.05).

Conclusions

Levels of specific inflammatory proteins may play a crucial role in triggering an immune response to PTB. Altered levels of LT and TNFRSF9 have the potential to serve as predictive markers for PTB development, necessitating further clinical validation in real-world settings to ascertain the impact of these inflammatory proteins on PTB.
Canine peripheral non-conventional TCRαβ+ CD4-CD8α- double-negative T cells show T helper 2-like and regulatory properties
Authors: Protschka Martina , Di Placido Daniela , Moore Peter F. , Büttner Mathias , Alber Gottfried , Eschke Maria
Published: May 2024

The dog is an important companion animal and also serves as model species for human diseases. Given the central role of T cells in immune responses, a basic understanding of canine conventional T cell receptor (TCR)αβ+ T cells, comprising CD4+ single-positive (sp) T helper (Th) and CD8α+ sp cytotoxic T cell subsets, is available. However, characterization of canine non-conventional TCRαβ+ CD4+CD8α+ double-positive (dp) and TCRαβ+ CD4−CD8α− double-negative (dn) T cells is limited. In this study, we performed a comprehensive analysis of canine dp and dn T cells in comparison with their conventional counterparts. TCRαβ+ T cells from peripheral blood of healthy dogs were sorted according to their CD4/CD8α phenotype into four populations (i.e. CD4+ sp, CD8α+ sp, dp, and dn) and selected surface markers, transcription factors and effector molecules were analyzed ex vivo and after in vitro stimulation by RT-qPCR. Novel characteristics of canine dp T cells were identified, expanding the previously characterized Th1-like phenotype to Th17-like and Th2-like properties. Overall, mRNA expression of various Th cell-associated cytokines (i.e. IFNG, IL17A, IL4, IL13) in dp T cells upon stimulation highlights their versatile immunological potential. Furthermore, we demonstrated that the CD4-CD8α- dn phenotype is stable during in vitro stimulation. Strikingly, dn T cells were found to express highest mRNA levels of type 2 effector cytokines (IL4, IL5, and IL13) upon stimulation. Their strong ability to produce IL-4 was confirmed at the protein level. Upon stimulation, the percentage of IL-4-producing cells was even higher in the non-conventional dn than in the conventional CD4+ sp population. Constitutive transcription of IL1RL1 (encoding IL-33Rα) further supports Th2-like properties within the dn T cell population. These data point to a role of dn T cells in type 2 immunity. In addition, the high potential of dn T cells to transcribe the gene encoding the co-inhibitory receptor CTLA-4 and to produce the inhibitory cytokine IL-10 indicates putative immunosuppressive capacity of this population. In summary, this study reveals important novel aspects of canine non-conventional T cells providing the basis for further studies on their effector and/or regulatory functions to elucidate their role in health and disease.
Clinical biomarker profiles reveals gender differences and mortality factors in sepsis
Authors: Chang Zhenglin , Lu Jiancai , Zhang Qitai , Wu Haojie , Liang Zhiman , Pan Xiaocong , Li Bishan , Cheng Zhangkai J. , Sun Baoqing
Published: May 2024

Background

Sepsis is a major contributor to global morbidity and mortality, affecting millions each year. Notwithstanding the decline in sepsis incidence and mortality over decades, gender disparities in sepsis outcomes persist, with research suggesting higher mortality rates in males.

Methods

This retrospective study aims to delineate gender-specific clinical biomarker profiles impacting sepsis progression and mortality by examining sepsis cases and related clinical data from the past three years. Propensity score matching was used to select age-matched healthy controls for comparison.

Results

Among 265 sepsis patients, a significantly higher proportion were male (60.8%, P<0.001). While mortality did not significantly differ by gender, deceased patients were significantly older (mean 69 vs 43 years, P=0.003), more likely to have hypertension (54% vs 25%, P=0.019), and had higher SOFA scores (mean ~10 vs 4, P<0.01) compared to survivors. Principal Component Analysis (PCA) showed clear separation between sepsis patients and healthy controls. 48 serum biomarkers were significantly altered in sepsis, with Triiodothyronine, Apolipoprotein A, and Serum cystatin C having the highest diagnostic value by ROC analysis. Gender-stratified comparisons identified male-specific (e.g. AFP, HDLC) and female-specific (e.g. Rheumatoid factor, Interleukin-6) diagnostic biomarkers. Deceased patients significantly differed from survivors, with 22 differentially expressed markers; Antithrombin, Prealbumin, HDL cholesterol, Urea nitrogen and Hydroxybutyrate had the highest diagnostic efficiency for mortality.

Conclusion

These findings enhance our understanding of gender disparities in sepsis and may guide future therapeutic strategies. Further research is warranted to validate these biomarker profiles and investigate the molecular mechanisms underlying these gender differences in sepsis outcomes.
The effect of exopolysaccharides from EMS-induced Porphyridium cruentum mutant on human epidermal and dermal layers
Authors: Han Sang-Il , Heo Young Mok , Jeon Min Seo , Kyung Seoyeon , Kang Seunghyun , Kwon Soon-Jae , Ryu Jai Hyunk , Kim Jae Hoon , Ahn Joon-Woo
Published: May 2024

Introduction

Microalgae biotechnology utilizes species like Porphyridium cruentum for their valuable phycobiliproteins and exopolysaccharides, which have potential industrial applications and health benefits, particularly in skin condition improvement.

Methods

A mutant of P. cruentum LIMS-PS-1061 was developed through ethyl methanesulfonate mutagenesis and subsequent colony screening to study changes in its biomass production and pigment composition under different lighting conditions.

Results and discussion

The mutant exhibited a 33.9% increase in dry weight under white light compared to the wild type. Despite maintaining the total pigment content, specific components changed significantly: chlorophyll content decreased 2.20- and 3.61-fold under white and blue light respectively, while phycobiliproteins increased 1.59- and 1.23-fold under the same conditions. These alterations suggest a compensatory mechanism for maintaining photosynthetic capacity. Furthermore, the exopolysaccharides of P. cruentum upregulated genes related to skin moisturization, barrier enhancement, and elasticity, and promoted wound healing through fibroblast migration. This supports the proposed mechanism of action for P. cruentum’s exopolysaccharides in improving human skin conditions by integrating the effects of aquaporin 3, filaggrin, involucrin, loricrin, elastin, and fibrillin-1.
A comprehensive genome survey study unveils the genomic characteristics and phylogenetic evolution of fishes in the Uranoscopidae family
Authors: Liu Qi , Zhao Xiang , Qu Yinquan , Wang Yiting , Guo Xingle , Li Wenyu , Gao Tianxiang , Qiao Ying
Published: May 2024

The construction of high-quality genomes is fundamental for molecular studies of species. Currently, there are no published genome sequences within the Uranoscopidae family, and research on the genomic characteristics of this family is lacking. In this study, genomic analyses of seven species from the Uranoscopidae family which captured from the southeastern coast of China were conducted using next-generation sequencing technology. The results revealed that the genomic characteristics of the seven species are relatively similar. The genome sizes of the seven Uranoscopidae species ranged from 536.00 Mb to 652.49 Mb, with repeat sequence proportions between 20.09% and 36.64%, and heterozygosity levels ranging from 0.41% to 0.88%. The assembled draft genomes exhibited GC contents of these species ranging from approximately 42.14% to 43.53%. Furthermore, the mitochondrial sequences for the seven species were assembled, with sizes ranging from 14,966 to 18,446 bp. The evolutionary relationships between different species were depicted through the mapping of conserved genes, whole-genome variances and mitochondrial genome sequences. Besides, the historical effective population sizes of all species experienced rapid expansion after the Last Interglacial Period, with three species undergoing bottleneck effects. In conclusion, this study provides a reference for the subsequent construction of high-quality genomes for species within the Uranoscopidae family and offers a rich data resource for further evolutionary research.
Exploring novel approaches in the systemic therapy of low-grade serous carcinoma of the ovary: a literature review
Authors: Giannecchini Giovanna Vieira , da Silva Jessé Lopes , de Oliveira Bretas Gustavo , dos Santos Alexssandra Lima Siqueira , Baltar Lais Fernandes Rodrigues , de Melo Andreia Cristina
Published: May 2024

By presenting a comprehensive analysis of low-grade serous carcinomas (LGSCs), a subset of epithelial ovarian cancers, this review delves into their distinct molecular characteristics, clinicopathological features and systemic therapy options, emphasizing their differences from high-grade serous carcinomas (HGSCs). Notably, LGSCs exhibit prevalent RAS/RAF/MEK/MAPK pathway activation, KRAS and BRAF mutations, and infrequent p53 mutations. While chemotherapy is commonly employed, LGSCs display lower responsiveness compared to HGSCs. Hormone therapy, particularly endocrine maintenance therapy, is explored due to the higher estrogen receptor expression. Novel therapeutic approaches involving CDK4/6 inhibitors, MEK inhibitors, and antiangiogenic agents like bevacizumab are also investigated. Ongoing clinical trials are striving to enhance LGSC treatment strategies, offering valuable insights for future therapeutic advancements in this challenging ovarian cancer subtype.
Correlation between vitamin D levels and blood pressure in elderly hypertensive patients with osteoporosis
Authors: Wu Tianlong , Lin Zebin , Wang Chizhen , Liu Xia
Published: May 2024

Objectives

The association between vitamin D and blood pressure in elderly patients with hypertension complicated by osteoporosis remains unclear. The objective of this study is to explore whether vitamin D deficiency contributes to elevated blood pressure in elderly individuals with both hypertension and osteoporosis.

Methods

This study represents a single-center retrospective observational investigation carried out at the Zhongshan Hospital Affiliated to Xiamen University. Ambulatory blood pressure, bone density, vitamin D levels, and additional laboratory parameters were collected upon admission. The association between vitamin D and ambulatory blood pressure outcomes was assessed using Spearman correlation tests and partial correlation analyses. The relationship between vitamin D and changes in blood pressure was analyzed through Generalized Additive Models, and threshold analysis was conducted to explore potential thresholds.

Results

139 patients with newly diagnosed osteoporosis were consecutively included (mean age 73 years, 84.9% female). There is a negative correlation between 25-(OH) D3 and 24 h mean systolic blood pressure (mSBP), diurnal mSBP, nocturnal mSBP, maximum SBP, respectively. The results of the generalized additive model analysis show that there is a nonlinear relationship between 25-(OH) D3 and 24 h mSBP, diurnal mSBP, nocturnal mSBP, respectively. After determining the critical point of 25-(OH) D3 as 42 nmol/L, a segmented linear regression model was used to calculate the effect size and 95% confidence interval on both sides of the critical point. When 25-(OH) D3 is ≤42 nmol/L, it significantly negatively correlates with 24 h, diurnal, and nocturnal mean SBP. Conversely, when 25-(OH) D3 exceeds 42 nmol/L, there is no statistically significant association with 24 h, diurnal, or nocturnal mSBP.

Conclusion

There was a significant negative correlation between vitamin D levels and blood pressure levels in elderly patients with hypertension and osteoporosis.
Gut microbiota associations with chronic kidney disease: insights into nutritional and inflammatory parameters
Authors: Lazarevic Vladimir , Teta Daniel , Pruijm Menno , Stoermann Catherine , Marangon Nicola , Mareschal Julie , Solano Raquel , Wurzner-Ghajarzadeh Arlene , Gaïa Nadia , Cani Patrice D. , Dizdar Oğuzhan S. , Herrmann François R. , Schrenzel Jacques , Genton Laurence
Published: May 2024

Introduction

The gut barrier, comprising gut microbiota, plays a pivotal role in chronic kidney disease (CKD) progression and nutritional status. This study aimed to explore gut barrier alterations in hemodialyzed (HD) patients, non-HD (NHD) CKD patients, and healthy volunteers.

Methods

Our cross-sectional study enrolled 22 HD patients, 11 NHD patients, and 11 healthy volunteers. We evaluated fecal microbiota composition (assessed via bacterial 16S rRNA gene sequencing), fecal IgA levels, surrogate markers of gut permeability, serum cytokines, appetite mediators, nutritional status, physical activity, and quality of life.

Results

HD patients exhibited significant alterations in fecal microbiota composition compared to healthy volunteers, with observed shifts in taxa known to be associated with dietary patterns or producing metabolites acting on human host. In comparison to healthy volunteers, individuals with HD patients exhibited elevated levels of inflammatory markers (CRP, IL-6 and TNF-α), glucagon-like peptide-2, and potential anorexigenic markers (including leptin and peptide YY). NHD patients had increased levels of CRP and peptide YY. Overall fecal microbiota composition was associated with height, soft lean mass, resting energy expenditure, handgrip strength, bone mineral content and plasma albumin and TNF-α.

Discussion

Compared to healthy volunteers, HD patients have an altered fecal microbiota composition, a higher systemic inflammation, and a modification in plasma levels of appetite mediators. While some differences align with previous findings, heterogeneity exists likely due to various factors including lifestyle and comorbidities. Despite limitations such as sample size, our study underscores the multifaceted interplay between gut microbiota, physiological markers, and kidney function, warranting further investigation in larger cohorts.
Testing dynamic correlations and nonlinearity in bivariate time series through information measures and surrogate data analysis
Authors: Pinto Helder , Lazic Ivan , Antonacci Yuri , Pernice Riccardo , Gu Danlei , Barà Chiara , Faes Luca , Rocha Ana Paula
Published: May 2024

The increasing availability of time series data depicting the evolution of physical system properties has prompted the development of methods focused on extracting insights into the system behavior over time, discerning whether it stems from deterministic or stochastic dynamical systems. Surrogate data testing plays a crucial role in this process by facilitating robust statistical assessments. This ensures that the observed results are not mere occurrences by chance, but genuinely reflect the inherent characteristics of the underlying system. The initial process involves formulating a null hypothesis, which is tested using surrogate data in cases where assumptions about the underlying distributions are absent. A discriminating statistic is then computed for both the original data and each surrogate data set. Significantly deviating values between the original data and the surrogate data ensemble lead to the rejection of the null hypothesis. In this work, we present various surrogate methods designed to assess specific statistical properties in random processes. Specifically, we introduce methods for evaluating the presence of autodependencies and nonlinear dynamics within individual processes, using Information Storage as a discriminating statistic. Additionally, methods are introduced for detecting coupling and nonlinearities in bivariate processes, employing the Mutual Information Rate for this purpose. The surrogate methods introduced are first tested through simulations involving univariate and bivariate processes exhibiting both linear and nonlinear dynamics. Then, they are applied to physiological time series of Heart Period (RR intervals) and respiratory flow (RESP) variability measured during spontaneous and paced breathing. Simulations demonstrated that the proposed methods effectively identify essential dynamical features of stochastic systems. The real data application showed that paced breathing, at low breathing rate, increases the predictability of the individual dynamics of RR and RESP and dampens nonlinearity in their coupled dynamics.
Low T3 syndrome is associated with the severity of myelin oligodendrocyte glycoprotein antibody-associated disease exacerbation
Authors: Wu Yuqing , Zhou Hao , Ci Xiaojiao , Lu Jie
Published: May 2024

Background

Myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) is a rare autoimmune inflammatory disease of the central nervous system, (CNS) different from multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD). While numerous studies have delved into the involvement of thyroid antibodies (ATAbs) and thyroid function in NMOSD and MS. The objective of this study is to explore the clinical significance of thyroid dysfunction and ATAbs abnormalities in adult patients with MOGAD.

Methods

36 adult inpatients diagnosed with MOGAD and 47 sex- and age-matched healthy controls were enrolled. Patients were divided into two groups based on the presence or absence of low T3 syndrome. Demographics, clinical characteristics, and results of auxiliary examinations were compared across the subgroups. Moreover, an analysis was conducted to explore the correlations between thyroid hormone levels and Expanded Disability Status Scale (EDSS) scores.

Results

Thyroid dysfunction was notably more frequent in MOGAD patients than healthy controls (p < 0.0001), particularly low T3 syndrome (p=0.03). Furthermore, subgroup analyses revealed that the low T3 syndrome group exhibited higher EDSS scores and a higher proportion of individuals with EDSS scores > 3, in comparison to the non-low T3 syndrome group (p = 0.014, p = 0.046). However, no significant differences were observed in demographic characteristics, annual relapse rates, clinical phenotypes, laboratory and MRI results, and EEG abnormalities between the two groups. Additional Spearman's analysis showed significantly negative correlations between the TT3 and FT3 levels with EDSS scores (r = −0.367, p = 0.028; r = −0.377, p = 0.024). Typical brain lesions and paralateral ventricle lesions were significantly rare in patients with positive ATAbs compared to those with negative ATAbs (p = 0.0001, p = 0.03), although the incidence of ATAbs abnormalities did not differ significantly between MOGAD patients and healthy controls.

Conclusions

Overall, this study confirmed thyroid dysfunction, especially low T3 syndrome, is frequent in adult MOGAD patients. Patients with low T3 syndrome exhibited elevated EDSS scores and a significantly higher incidence of unfavorable condition. additionally, the correlation analysis model manifests that FT3 and TT3 levels were negatively correlated with EDSS scores. These evidences indicate that low T3 syndrome is associated with the severity of MOGAD exacerbation.