bluuebunny/crossref_metadata_2025 huggingface dataset:
The CRBN, CUL4A and DDB1 Expression Predicts the Response to Immunomodulatory Drugs and Survival of Multiple Myeloma Patients
Authors: Barankiewicz Joanna , Szumera-Ciećkiewicz Anna , Salomon-Perzyński Aleksander , Wieszczy Paulina , Malenda Agata , Garbicz Filip , Prochorec-Sobieszek Monika , Misiewicz-Krzemińska Irena , Juszczyński Przemysław , Lech-Marańda Ewa
Published: June 2021

Immunomodulatory drugs (IMiDs) are effective in the treatment of multiple myeloma (MM), myelodysplastic syndrome with deletion of chromosome 5q and other haematological malignancies. Recent studies showed that IMiDs bind to cereblon (CRBN), a substrate receptor of the CRL4–CRBN complex, to induce the ubiquitination and degradation of IKZF1 and IKZF3 in MM cells, contributing to their anti-myeloma activity. We aimed to determine whether the CRL4–CRBN complex proteins’ expression predicts the prognosis of MM patients treated with IMiDs. Here, we evaluated the expression of CRL4–CRBN complex proteins and their downstream targets with immunohistochemistry (IHC) staining in 130 bone marrow samples from MM patients treated with thalidomide or lenalidomide-based regimens. We found that the expression of CRBN and CUL4A was associated with the superior IMiD-based treatment response (p = 0.007 and p = 0.007, respectively). Moreover, the CUL4A expression was associated with improved PFS (HR = 0.66, 95% CI 0.44–0.99; p = 0.046) and DDB1 expression showed a negative impact on OS both in the univariate (HR = 2.75, 95% CI 1.65–4.61; p = 0.001) and the multivariate (HR 3.67; 95% CI 1.79–7.49; p < 0.001) analysis. Overall, our data suggest that the expression of DDB1, CUL4A and CRBN assessed by IHC predicts the clinical course of MM patients and identifies patients with a high probability of responding to IMiD-based therapy.
Validation of the Center of Applied Psychology Female Sexuality Questionnaire (CAPFS-Q)
Authors: Tirado-González Sonia , Navarro-Sánchez Antonio , Compañ-Rosique Antonio , Luri-Prieto Paloma , Rodríguez-Marín Jesús , Van-der Hofstadt-Román Carlos J. , Berenguer Soler María , Navarro-Cremades Felipe , Gil-Guillén Vicente F. , Navarro Ortiz Ramón , Montejo Angel L. , Pérez-Jover Virtudes
Published: June 2021

Instruments for the measurement of human sexuality include self-report measures used to assess sexual functioning, but many of them have not yet been validated. The Center of Applied Psychology Female Sexual Questionnaire (CAPFS-Q) is an original self-report instrument. It has been developed for the study of sexuality in specific non-clinical populations, such as female university students of Medicine and other Health Sciences. The CAPFS-Q includes 26 items, organized as follows: sociodemographic and relevant data (four items); aspects of sexual relations with partner (five items); sexual practices (12 from 13 items); and dysfunctional aspects of sexual relations (four items). CAPFS-Q validity and reliability were examined in a sample of Spanish female university students of Health Sciences. Exploratory and confirmatory factor analysis (FA) showed a four-factor structure which explained 71.6% of the variance. This initial version of the CAPFS-Q is a reliable measure of women’s sexual behavior, with a dimensionality that replicates the initial theoretical content and with adequate indicators of internal consistency, validity, and test–retest reliability. It is easy to administer and to complete.
Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA
Authors: Aydos Kaan , Aydos Oya Sena
Published: June 2021

Retrieving spermatozoa from the testicles has been a great hope for patients with non-obstructive azoospermia (NOA), but relevant methods have not yet been developed to the level necessary to provide resolutions for all cases of NOA. Although performing testicular sperm extraction under microscopic magnification has increased sperm retrieval rates, in vitro selection and processing of quality sperm plays an essential role in the success of in vitro fertilization. Moreover, sperm cryopreservation is widely used in assisted reproductive technologies, whether for therapeutic purposes or for future fertility preservation. In recent years, there have been new developments using advanced technologies to freeze and preserve even very small numbers of sperm for which conventional techniques are inadequate. The present review provides an up-to-date summary of current strategies for maximizing sperm recovery from surgically obtained testicular samples and, as an extension, optimization of in vitro sperm processing techniques in the management of NOA.
Interest of Follow-Up Radiological Imaging in Patients with Pyogenic Vertebral Osteomyelitis
Authors: Hecquet Sophie , Verhoeven Frank , Aubry Sébastien , Prati Clément , Wendling Daniel , Chirouze Catherine , Bouiller Kévin
Published: June 2021

No recommendations are established for monitoring pyogenic vertebral osteomyelitis (PVO). Thus, the realization of systematic follow-up radiological imaging is controversial. The objective of this study was to evaluate the interest in follow-up radiological imaging in patients with PVO. We conducted a retrospective cohort analysis of patients with PVO who had both baseline and follow-up radiological imaging. We classified the follow-up images into two groups, improvement/stability, and deterioration, compared with the baseline data. For each patient, we compared their radiological imaging follow-up to their clinical-biological condition assessed at the same time. Eighty-six patients were included. The mean age was 68 years (±13). A total of 99 radiological imaging examinations at diagnosis and at follow-up were analyzed, 69 Magnetic Resonance Imaging (MRI), and 30 Computerized Tomography (CT scans). The mean delay between the follow-up radiological imaging and clinical evaluation was 2.8 +/− 2.1 months. Of the 36 patients with clinical and biological recovery, 24 patients (67%) had improved radiological imaging and 12 patients (34%) had radiological worsening (new abscesses (n = 4), extension of soft tissue infiltration (n = 2) and/or epiduritis (n = 2) or appearance of new locations (n = 1)). Among the 50 patients considered as unhealed, on the contrary, radiological imaging showed an improvement in imaging in 39 patients (78%) and a worsening in 11 patients (22%). Our study showed that there was no correlation between the clinical condition of patients and their follow-up radiological imaging in the context of PVO.
Oxidative Stress in Cutaneous Lichen Planus—A Narrative Review
Authors: Georgescu Simona Roxana , Mitran Cristina Iulia , Mitran Madalina Irina , Nicolae Ilinca , Matei Clara , Ene Corina Daniela , Popa Gabriela Loredana , Tampa Mircea
Published: June 2021

Lichen planus (LP) is a chronic, immune-mediated inflammatory skin condition that mainly affects the skin (cutaneous LP, CLP) and oral mucosa (oral LP, OLP). However, the mechanisms involved in the pathogenesis of the disease are not fully elucidated. Over time, several theories that could explain the appearance of LP lesions have been postulated. The key players in LP pathogenesis are the inflammatory infiltrate consisting of T cells and the proinflammatory cytokines. The cytokines stimulate the production of reactive oxygen species that induce cell apoptosis, a defining element encountered in LP. The lead inquiry triggered by this revolves around the role of oxidative stress in LP development. There are currently numerous studies showing the involvement of oxidative stress in OLP, but in terms of CLP, data are scarce. In this review, we analyze for the first time the currently existing studies on oxidative stress in CLP and summarize the results in order to assess the role of oxidative stress in skin lesions offering a fresher updated perspective.
Characteristics, Location, and Clinical Outcomes of Gastrointestinal Bleeding in Patients Taking New Oral Anticoagulants Compared to Vitamin K Antagonists
Authors: Choe A Reum , Moon Chang Mo , Tae Chung Hyun , Chun Jaeyoung , Bang Ki Bae , Lee Yoo Jin , Lee Hyun Seok , Jung Yunho , Park Sung Chul , Koo Hoon Sup
Published: June 2021

New oral anticoagulants (NOACs) are commonly used in clinical practice as alternatives to vitamin K antagonists (VKA). However, the etiology, clinical course, and risk of gastrointestinal (GI) bleeding remain unclear. We aimed to evaluate the clinical characteristics and location of acute GI bleeding associated with NOACs and its severity and outcomes compared to VKA. This retrospective multicenter study included 381 subjects on anticoagulants who underwent appropriate diagnostic examination due to GI bleeding. Regarding the characteristics of acute GI bleeding, the proportion of vascular lesions was significantly lower in the NOACs group than that in the VKA group. Small bowel bleeding occurred less commonly in the NOACs group, but the difference did not reach statistical significance. Regarding severity and clinical outcomes, patients on NOACs received significantly smaller volumes of transfused blood products and had shorter ICU stays than those on VKA. Moreover, the need for surgery and the risk of rebleeding in the NOACs group were significantly lower than those in the VKA group. Patients on NOACs have better clinical outcomes in terms of severity of acute GI bleeding or rebleeding than patients on VKA. Patients on NOACs demonstrate different characteristics and location of acute GI bleeding than those on VKA.
Low-Intensity Continuous Ultrasound Therapies—A Systematic Review of Current State-of-the-Art and Future Perspectives
Authors: Uddin Sardar , Komatsu David , Motyka Thomas , Petterson Stephanie
Published: June 2021

Therapeutic ultrasound has been studied for over seven decades for different medical applications. The versatility of ultrasound applications are highly dependent on the frequency, intensity, duration, duty cycle, power, wavelength, and form. In this review article, we will focus on low-intensity continuous ultrasound (LICUS). LICUS has been well-studied for numerous clinical disorders, including tissue regeneration, pain management, neuromodulation, thrombosis, and cancer treatment. PubMed and Google Scholar databases were used to conduct a comprehensive review of all research studying the application of LICUS in pre-clinical and clinical studies. The review includes articles that specify intensity and duty cycle (continuous). Any studies that did not identify these parameters or used high-intensity and pulsed ultrasound were not included in the review. The literature review shows the vast implication of LICUS in many medical fields at the pre-clinical and clinical levels. Its applications depend on variables such as frequency, intensity, duration, and type of medical disorder. Overall, these studies show that LICUS has significant promise, but conflicting data remain regarding the parameters used, and further studies are required to fully realize the potential benefits of LICUS.
Optimal Upfront Treatment in Surgically Resectable Pancreatic Cancer Candidates: A High-Volume Center Retrospective Analysis
Authors: Maloney Sarah , Itchins Malinda , Arena Jennifer , Sahni Sumit , Howell Viive M. , Hayes Sarah A. , Gill Anthony J. , Clarke Stephen J. , Samra Jaswinder , Mittal Anubhav , Pavlakis Nick
Published: June 2021

Pancreatic adenocarcinoma is a devastating disease with only 15–20% of patients resectable at diagnosis. Neoadjuvant chemotherapy for this cohort is becoming increasingly popular; however, there are no published randomized trials that support the use of neoadjuvant chemotherapy over upfront surgery in resectable disease. This retrospective cohort analysis was conducted to compare both treatment pathways and to identify any potential prognostic markers. Medical records from one large volume pancreatic cancer center from 2013–2019 were reviewed and 126 patients with upfront resectable disease were analyzed. Due to a change in practice in our center patients treated prior to December 2016 received upfront surgery and those treated after this date received neoadjuvant chemotherapy. Of these, 86 (68%) patients were treated with upfront surgery and 40 (32%) of patients were treated with neoadjuvant chemotherapy. Our results demonstrated that patients treated with upfront surgery with early-stage (1a) disease had a longer median OS compared to those treated with neoadjuvant chemotherapy (24 vs. 21 months, p = 0.028). This survival difference was not evident for all patients (regardless of stage). R0 resections were similar between groups (p = 0.605). We identified that both tumor viability (in neoadjuvant chemotherapy-treated patients) and tumor grade were useful prognostic markers. Upfront surgery for certain patients with low volume disease may be suitable despite the global trend towards neoadjuvant chemotherapy for all upfront resectable patients. A prospective clinical trial in this cohort incorporating biomarkers is needed to determine optimal therapy pathway.
Fungal Melanins and Applications in Healthcare, Bioremediation and Industry
Authors: Mattoon Ellie Rose , Cordero Radames J. B. , Casadevall Arturo
Published: June 2021

Melanin is a complex multifunctional pigment found in all kingdoms of life, including fungi. The complex chemical structure of fungal melanins, yet to be fully elucidated, lends them multiple unique functions ranging from radioprotection and antioxidant activity to heavy metal chelation and organic compound absorption. Given their many biological functions, fungal melanins present many possibilities as natural compounds that could be exploited for human use. This review summarizes the current discourse and attempts to apply fungal melanin to enhance human health, remove pollutants from ecosystems, and streamline industrial processes. While the potential applications of fungal melanins are often discussed in the scientific community, they are successfully executed less often. Some of the challenges in the applications of fungal melanin to technology include the knowledge gap about their detailed structure, difficulties in isolating melanotic fungi, challenges in extracting melanin from isolated species, and the pathogenicity concerns that accompany working with live melanotic fungi. With proper acknowledgment of these challenges, fungal melanin holds great potential for societal benefit in the coming years.
Recent Advances in Tumor Targeting via EPR Effect for Cancer Treatment
Authors: Subhan Md Abdus , Yalamarty Satya Siva Kishan , Filipczak Nina , Parveen Farzana , Torchilin Vladimir P.
Published: June 2021

Cancer causes the second-highest rate of death world-wide. A major shortcoming inherent in most of anticancer drugs is their lack of tumor selectivity. Nanodrugs for cancer therapy administered intravenously escape renal clearance, are unable to penetrate through tight endothelial junctions of normal blood vessels and remain at a high level in plasma. Over time, the concentration of nanodrugs builds up in tumors due to the EPR effect, reaching several times higher than that of plasma due to the lack of lymphatic drainage. This review will address in detail the progress and prospects of tumor-targeting via EPR effect for cancer therapy.
A Personalized 3D-Printed Model for Obtaining Informed Consent Process for Thyroid Surgery: A Randomized Clinical Study Using a Deep Learning Approach with Mesh-Type 3D Modeling
Authors: Seok Jungirl , Yoon Sungmin , Ryu Chang Hwan , Kim Seok-ki , Ryu Junsun , Jung Yuh-Seog
Published: June 2021

The aim of this study was to evaluate the usefulness of a personalized 3D-printed thyroid model that characterizes a patient’s individual thyroid lesion. The randomized controlled prospective clinical trial (KCT0005069) was designed. Fifty-three of these patients undergoing thyroid surgery were randomly assigned to two groups: with or without a 3D-printed model of their thyroid lesion when obtaining informed consent. We used a U-Net-based deep learning architecture and a mesh-type 3D modeling technique to fabricate the personalized 3D model. The mean 3D printing time was 258.9 min, and the mean price for production was USD 4.23 for each patient. The size, location, and anatomical relationship of the tumor and thyroid gland could be effectively presented using the mesh-type 3D modeling technique. The group provided with personalized 3D-printed models showed significant improvement in all four categories (general knowledge, benefits and risks of surgery, and satisfaction; all p < 0.05). All patients received a personalized 3D model after surgery and found it helpful to understand the disease, operation, and possible complications and their overall satisfaction (all p < 0.05). In conclusion, the personalized 3D-printed thyroid model may be an effective tool for improving a patient’s understanding and satisfaction during the informed consent process.
Detection of Aberrant Glycosylation of Serum Haptoglobin for Gastric Cancer Diagnosis Using a Middle-Up-Down Glycoproteome Platform
Authors: Jeong Seunghyup , Kim Unyong , Oh Myung , Nam Jihyeon , Park Se , Choi Yoon , Lee Dong , Kim Jaehan , An Hyun
Published: June 2021

Gastric cancer is a frequently occurring cancer and is the leading cause of cancer-related deaths. Recent studies have shown that aberrant glycosylation of serum haptoglobin is closely related to gastric cancer and has enormous potential for use in diagnosis. However, there is no platform with high reliability and high reproducibility to comprehensively analyze haptoglobin glycosylation covering microheterogeneity to macroheterogeneity for clinical applications. In this study, we developed a middle-up-down glycoproteome platform for fast and accurate monitoring of haptoglobin glycosylation. This platform utilizes an online purification of LC for sample desalting, and an in silico haptoglobin glycopeptide library constructed by combining peptides and N-glycans to readily identify glycopeptides. In addition, site-specific glycosylation with glycan heterogeneity can be obtained through only a single MS analysis. Haptoglobin glycosylation in clinical samples consisting of healthy controls (n = 47) and gastric cancer patients (n = 43) was extensively investigated using three groups of tryptic glycopeptides: GP1 (including Asn184), GP2 (including Asn207 and Asn211), and GP3 (including Asn241). A total of 23 individual glycopeptides were determined as potential biomarkers (p < 0.00001). In addition, to improve diagnostic efficacy, we derived representative group biomarkers with high AUC values (0.929 to 0.977) through logistic regression analysis for each GP group. It has been found that glycosylation of haptoglobin is highly associated with gastric cancer, especially the glycosite Asn241. Our assay not only allows to quickly and easily obtain information on glycosylation heterogeneity of a target glycoprotein but also makes it an efficient tool for biomarker discovery and clinical diagnosis.
Identifying Urban Poverty Using High-Resolution Satellite Imagery and Machine Learning Approaches: Implications for Housing Inequality
Authors: Li Guie , Cai Zhongliang , Qian Yun , Chen Fei
Published: June 2021

Enriching Asian perspectives on the rapid identification of urban poverty and its implications for housing inequality, this paper contributes empirical evidence about the utility of image features derived from high-resolution satellite imagery and machine learning approaches for identifying urban poverty in China at the community level. For the case of the Jiangxia District and Huangpi District of Wuhan, image features, including perimeter, line segment detector (LSD), Hough transform, gray-level cooccurrence matrix (GLCM), histogram of oriented gradients (HoG), and local binary patterns (LBP), are calculated, and four machine learning approaches and 25 variables are applied to identify urban poverty and relatively important variables. The results show that image features and machine learning approaches can be used to identify urban poverty with the best model performance with a coefficient of determination, R2, of 0.5341 and 0.5324 for Jiangxia and Huangpi, respectively, although some differences exist among the approaches and study areas. The importance of each variable differs for each approach and study area; however, the relatively important variables are similar. In particular, four variables achieved relatively satisfactory prediction results for all models and presented obvious differences in varying communities with different poverty levels. Housing inequality within low-income neighborhoods, which is a response to gaps in wealth, income, and housing affordability among social groups, is an important manifestation of urban poverty. Policy makers can implement these findings to rapidly identify urban poverty, and the findings have potential applications for addressing housing inequality and proving the rationality of urban planning for building a sustainable society.
Struggles of Women to Access and Hold Landuse and Other Land Property Rights under the Customary Tenure System in Peri-Urban Communal Areas of Zimbabwe
Authors: Ingwani Emaculate
Published: June 2021

The struggles of women to access and hold landuse and other land property rights under the customary tenure system in peri-urban communal areas is increasingly becoming a cause for concern. These debates are revealed using a case study of a peri-urban communal area called Domboshava in Zimbabwe. Women living in this peri-urban communal area struggle to access and hold landuse and other land property rights registered under their names. The aim of this paper is to present an analysis of the struggles faced by women to access and hold landuse and other land property rights in Domboshava. This paper is a product of a literature review on land property rights, land tenure systems, and peri-urbanity more generally. Field data was intermittently collected in the peri-urban communal area of Domboshava over a period of four years from 2011 to 2014, as well as through post-research social visits stretching to 2019. Thirty-two women were conveniently selected and interviewed. I applied Anthony Giddens’ structure-agency theory as a framework of analysis. The struggles to access and hold landuse and other land property rights by women are rooted in land transactions, social systems including the customary land tenure, patriarchy, as well as the peri-urban context of Domboshava. Responsible authorities on land administration in communal areas need to acknowledge the existence of new and invented ways of accessing and holding landuse and land property rights under the customary land tenure system, as well as to find ways to mobilize more opportunities for women on the peri-urban land market.
Tracking Deformation Processes at the Legnica Glogow Copper District (Poland) by Satellite InSAR—I: Room and Pillar Mine District
Authors: Antonielli Benedetta , Sciortino Alessandra , Scancella Stefano , Bozzano Francesca , Mazzanti Paolo
Published: June 2021

Mining exploitation leads to slow or rapid ground subsidence resulting from deformation until the collapse of underground post-mining voids following excavation activities. Satellite SAR interferometry capabilities for the evaluation of ground movements allows the monitoring of intensive surface mine subsidence and can provide new knowledge about the risks in the mining industry. This work integrates both conventional and advanced Differential SAR Interferometry (DInSAR) to study the ground subsidence in the Legnica Glogow Copper District (LGCD, Poland) by processing about 400 Sentinel-1 images from October 2014 to April 2019. Even without field data and information on past and ongoing excavation activities, the DInSAR approach allowed us to identify 30 troughs of subsidence, ranging from 500 m to 2.5 km in diameter, which in some cases, took place several times during the analyzed time span. The cumulative subsidence in 4 years and 7 months exceeds 70 cm in several zones of the LGCD. The sub-centimetric precision achieved by advanced analysis (A-DInSAR), allowed us to monitor the real extent of the mining influence area on the surface, with deformation velocities of up to 50 mm/year. The ground deformation detected at LGCD can be due to both mining-induced tremors and roof subsidence above the underground excavation rooms. As deformations do not occur concurrently with tremors, this can be related to excavation activities or to degradation of abandoned mines.
An Unintended Legacy: The External Policy Responses of the USA and European Union to Conflict Minerals in Africa
Authors: Addaney Michael , Lubaale Emma Charlene
Published: June 2021

Competition over environmental and natural resources characteristically lies at the heart of armed conflicts in Africa. It is also common knowledge that some companies dealing in products such as laptops, smart phones, and jewellery import minerals from conflict-affected areas, thereby indirectly fuelling conflicts in these areas or undermining human rights. For a continent endowed with natural resources including minerals, Africa has suffered the brunt of this predicament. This state of affairs has lent impetus to the adoption of several regulations geared towards curbing irresponsible business practices by companies relying on such minerals, the goal being, amongst others, to guarantee the protection of human rights. In May 2017, the European Union adopted regulations intended to stop the importation of conflict minerals in Europe, debatably making giant strides in the direction of the protection of human rights. These regulations are to come into force in 2021. However, can these regulations advance the much-desired goal of the protection of human rights in Africa on issues pertaining to conflict minerals? By analyzing the 2017 EU regulations in light of previous regulations of a similar nature, the paper concludes that the said regulations constitute a weak normative framework and could in fact have unintended consequences on the fundamental rights of civilians in natural resource-rich conflict areas of Africa.
Aerobic Exercise Decreases Negative Affect by Modulating Orbitofrontal-Amygdala Connectivity in Adolescents
Authors: Ge Li-Kun , Hu Zhuoer , Wang Weiwen , Siu Parco M. , Wei Gao-Xia
Published: June 2021

Long-term negative affect in adolescence is associated with impairment in quality of life, interpersonal function, and social adaptation. Although physical exercise could decrease negative emotion, the underlying mechanism remains largely unknown. Acute exercise with controlled intensity might be a good experimental paradigm to unravel the potential neural mechanisms underlying the effects of physical exercise on negative affect. In this study, twenty-three males in late adolescence were randomly assigned to acute exercise group (AG) or control group. The experiment contained pre-test and post-test session interleaved with 30-min moderate-intensity exercise or seated rest. In each session, a resting-state fMRI scanning was conducted followed by completing Positive and Negative Affect Schedule and Profile of Mood State. Bilateral amygdala was used as seed region to calculate t voxel-wised functional connectivity (FC) of amygdala to whole brain. The results demonstrated, for the first time, that AG exhibited increased FC between right amygdala and right orbital frontal cortex. Significantly decreased negative affect was also observed in AG. Moreover, the increased rOFC-amygdala FC was also associated with the decreased depression score. Our findings suggest that exercise-induced decreased negative affect might be modulated by functional interactions of amygdala with both cognitive control and limbic networks, which offers a meaningful insight for clinical treatment and prevention of emotional disorders in late adolescence.
Comparative Analysis of Flavonoid Metabolites in Foxtail Millet (Setaria italica) with Different Eating Quality
Authors: Zhang Yakun , Gao Jianhua , Qie Qianru , Yang Yulu , Hou Siyu , Wang Xingchun , Li Xukai , Han Yuanhuai
Published: June 2021

Foxtail millet (Setaria italica) is an important minor cereal crop in China. The yellow color of the de-husked grain is the most direct aspect for evaluating the foxtail millet quality. The yellow pigment mainly includes carotenoids (lutein and zeaxanthin) and flavonoids. To reveal the diversity and specificity of flavonoids in foxtail millet, we chose three high eating quality and two poor eating quality varieties as research materials. A total of 116 flavonoid metabolites were identified based on Ultra Performance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (UPLC-ESI-MS/MS) system. The tested varieties contained similar levels of flavonoid metabolites, but with each variety accumulating its unique flavonoid metabolites. A total of 33 flavonoid metabolites were identified as significantly discrepant between high eating quality and poor eating quality varieties, which were mainly in the flavonoid biosynthesis pathway and one of its branches, the flavone and flavonol biosynthesis pathway. These results showed the diversified components of flavonoids accumulated in foxtail millets and laid the foundation for further research on flavonoids and the breeding for high-quality foxtail millet varieties.
Hyperalphalipoproteinemia and Beyond: The Role of HDL in Cardiovascular Diseases
Authors: Giammanco Antonina , Noto Davide , Barbagallo Carlo Maria , Nardi Emilio , Caldarella Rosalia , Ciaccio Marcello , Averna Maurizio Rocco , Cefalù Angelo Baldassare
Published: June 2021

Hyperalphalipoproteinemia (HALP) is a lipid disorder characterized by elevated plasma high-density lipoprotein cholesterol (HDL-C) levels above the 90th percentile of the distribution of HDL-C values in the general population. Secondary non-genetic factors such as drugs, pregnancy, alcohol intake, and liver diseases might induce HDL increases. Primary forms of HALP are caused by mutations in the genes coding for cholesteryl ester transfer protein (CETP), hepatic lipase (HL), apolipoprotein C-III (apo C-III), scavenger receptor class B type I (SR-BI) and endothelial lipase (EL). However, in the last decades, genome-wide association studies (GWAS) have also suggested a polygenic inheritance of hyperalphalipoproteinemia. Epidemiological studies have suggested that HDL-C is inversely correlated with cardiovascular (CV) risk, but recent Mendelian randomization data have shown a lack of atheroprotective causal effects of HDL-C. This review will focus on primary forms of HALP, the role of polygenic inheritance on HDL-C, associated risk for cardiovascular diseases and possible treatment options.
Mineral Layer Fillers for the Production of Functional Materials
Authors: Gerasimova Lidia G. , Maslova Marina V. , Shchukina Ekaterina S.
Published: June 2021

An original method based on the use of technogenic waste from the processing of mineral-layered materials, in particular phlogopite for obtaining highly efficient functional compositions of the “mica-TiO2”, has been developed. The composition core is a nanosized mica flake coated with mesoporous titanium dioxide of an anatase or rutile structure. Energy-saving and environmentally friendly technological methods are based on the splitting of the mica followed by heterogeneous electrohydrolysis of a mixture of titanium (IV) sulfate solution and flake particles. No destruction of the mica surface, which provided the obtained uniform coatings, has been observed. Such coatings are used in photocatalysis processes and possess a self-cleaning capability. Core–shell compositions are more economically attractive compared with titanium dioxide, in particular TiO2 grade P25 (Degusse). The core of the transparent flake and the shell of the rutile titanium dioxide endows the final product with a pearlescent optical effect. This type of material is widely used in the manufacturing of paints and varnishes, printing inks, cosmetics, etc. The use of technogenic waste could significantly reduce the cost of the final product, which would ensure its widespread use in various industries.
Synthesis and Physicochemical Evaluation of Bees’ Chitosan-Based Hydrogels Modified with Yellow Tea Extract
Authors: Kudłacik-Kramarczyk Sonia , Drabczyk Anna , Głąb Magdalena , Gajda Paweł , Jaromin Anna , Czopek Anna , Zagórska Agnieszka , Tyliszczak Bożena
Published: June 2021

The novelty of the research involves designing the measurement methodology aimed at determining the structure–property relationships in the chitosan-based hydrogels containing yellow tea extract. Performed investigations allowed us to determine the swelling properties of hydrogels in selected time intervals, evaluate the mutual interactions between the hydrogels and simulated physiological liquids via pH measurements and directly assess the impact of such interactions on the chemical structure of hydrogels using Fourier transform infrared (FT-IR) spectroscopy and their wettability by the measurements of the flatness of the drop on the surface of the tested samples via the static drop method. Next, the surface morphology of hydrogels was characterized by the Scanning Electron Miscorcopy (SEM) and their elasticity under the tension applied was also verified. It was proved that incubation in simulated physiological liquids resulted in a decrease in contact angles of hydrogels, even by 60%. This also caused their certain degradation which was reflected in lower intensities of bands on FT-IR spectra. Further, 23% v/v yellow tea extract in hydrogel matrices caused the decrease of their tensile strength. An increase in the amount of the crosslinker resulted in a decrease in the sorption capacity of hydrogels wherein their modification caused greater swelling ability. In general, the investigations performed provided much information on the tested materials which may be meaningful considering their application, e.g., as dressing materials.
Effectiveness of a Long-Term Training Programme for Teachers in Attention-Deficit/Hyperactivity Disorder on Knowledge and Self-Efficacy
Authors: Jarque Fernández Sonia , Amado Luz Laura , Oporto Alonso Marta , Fernández-Andújar Marina
Published: June 2021

The active participation of teachers in the process of diagnosis and intervention of Attention-Deficit/Hyperactivity Disorder (ADHD) is relevant to helping mitigate future problems in children with ADHD. In training programmes, teachers usually implement strategies to improve the child’s functioning after participating in an ADHD management-training programme. However, they receive little psycho-educational training and coaching and have low-to-moderate levels of knowledge of the disorder, mostly in terms of training in classroom management strategies. This study analyses the effectiveness of training in increasing knowledge and perceived self-efficacy with regard to ADHD in 40 primary school teachers. Twenty of these teachers participated in a long-term psycho-educational training programme on the management of ADHD (intervention multimodal group), while the other 20 did not (control group). The results of the post-treatment phase revealed statistically significant differences between the two groups of teachers with regard to their knowledge (Z = −5.427; p = 0.000; d = 0.89) and perceived self-efficacy (Z = −5.150; p = 0.000; d = 0.79), in favour of the participants who had received training. Our findings have important implications for the design of training programmes for teachers in the management of ADHD in the classroom.
Fuzzy Clustering Methods with Rényi Relative Entropy and Cluster Size
Authors: Bonilla Javier , Vélez Daniel , Montero Javier , Rodríguez J. Tinguaro
Published: June 2021

In the last two decades, information entropy measures have been relevantly applied in fuzzy clustering problems in order to regularize solutions by avoiding the formation of partitions with excessively overlapping clusters. Following this idea, relative entropy or divergence measures have been similarly applied, particularly to enable that kind of entropy-based regularization to also take into account, as well as interact with, cluster size variables. Particularly, since Rényi divergence generalizes several other divergence measures, its application in fuzzy clustering seems promising for devising more general and potentially more effective methods. However, previous works making use of either Rényi entropy or divergence in fuzzy clustering, respectively, have not considered cluster sizes (thus applying regularization in terms of entropy, not divergence) or employed divergence without a regularization purpose. Then, the main contribution of this work is the introduction of a new regularization term based on Rényi relative entropy between membership degrees and observation ratios per cluster to penalize overlapping solutions in fuzzy clustering analysis. Specifically, such Rényi divergence-based term is added to the variance-based Fuzzy C-means objective function when allowing cluster sizes. This then leads to the development of two new fuzzy clustering methods exhibiting Rényi divergence-based regularization, the second one extending the first by considering a Gaussian kernel metric instead of the Euclidean distance. Iterative expressions for these methods are derived through the explicit application of Lagrange multipliers. An interesting feature of these expressions is that the proposed methods seem to take advantage of a greater amount of information in the updating steps for membership degrees and observations ratios per cluster. Finally, an extensive computational study is presented showing the feasibility and comparatively good performance of the proposed methods.
Electrochemistry, Electrodeposition, and Photoluminescence of Eu (III)/Lanthanides (III) on Terpyridine-Functionalized Ti Nanospikes
Authors: Joo Min Hee , Park So Jeong , Jang Hye Ji , Hong Sung-Min , Rhee Choong Kyun , Sohn Youngku
Published: June 2021

Terpyridine-functionalized Ti nanospike electrodes (TiNS-SiTpy) were developed and applied to cyclic voltammetry and amperometry of Ln (La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, and Yb) ions and mixed Eu (III) + Ln (III) ions in a 0.1 M NaClO4 electrolyte. Electrodeposition was successfully performed over TiNS-SiTpy electrodes, which were fully examined by scanning electron microscopy, X-ray diffraction crystallography, Fourier-transform infrared spectroscopy, energy dispersive X-ray spectroscopy, X-ray photoelectron spectroscopy, photoluminescence (PL), and PL decay kinetics. The Gd and Tb ions were found to increase PL intensities with 10× longer lifetimes of 1.32 μs and 1.03 μs, respectively, compared with that of the electrodeposited Eu sample. The crystal phase and the oxidation states were fully examined for the mixed Ln (Eu + Gd and Eu + Tb) complex structures.
Advances in Understanding of the Application of Unit Operations in Metallurgy of Rare Earth Elements
Authors: Stopic Srecko , Friedrich Bernd
Published: June 2021

Unit operations (UO) are mostly used in non-ferrous extractive metallurgy (NFEM) and usually separated into three categories: (1) hydrometallurgy (leaching under atmospheric and high pressure conditions, mixing of solution with gas and mechanical parts, neutralization of solution, precipitation and cementation of metals from solution aiming purification, and compound productions during crystallization), (2) pyrometallurgy (roasting, smelting, refining), and (3) electrometallurgy (aqueous electrolysis and molten salt electrolysis). The high demand for critical metals, such as rare earth elements (REE), indium, scandium, and gallium raises the need for an advance in understanding of the UO in NFEM. The aimed metal is first transferred from ores and concentrates to a solution using a selective dissolution (leaching or dry digestion) under an atmospheric pressure below 1 bar at 100 °C in an agitating glass reactor and under a high pressure (40–50 bar) at high temperatures (below 270 °C) in an autoclave and tubular reactor. The purification of the obtained solution was performed using neutralization agents such as sodium hydroxide and calcium carbonate or more selective precipitation agents such as sodium carbonate and oxalic acid. The separation of metals is possible using liquid (water solution)/liquid (organic phase) extraction (solvent extraction (SX) in mixer-settler) and solid-liquid filtration in chamber filter-press under pressure until 5 bar. Crystallization is the process by which a metallic compound is converted from a liquid into a crystalline state via a supersaturated solution. The final step is metal production using different methods (aqueous electrolysis for basic metals such as copper, zinc, silver, and molten salt electrolysis for REE and aluminum). Advanced processes, such as ultrasonic spray pyrolysis, microwave assisted leaching, and can be combined with reduction processes in order to produce metallic powders. Some preparation for the leaching process is performed via a roasting process in a rotary furnace, where the sulfidic ore was first oxidized in an oxidic form which is a suitable for the metal transfer to water solution. UO in extractive metallurgy of REE can be successfully used not only for the metal wining from primary materials, but also for its recovery from secondary materials.
Differences in Clinical and Dietary Characteristics, Serum Adipokine Levels, and Metabolomic Profiles between Early- and Late-Onset Gout
Authors: Suh Young Sun , Noh Hae Sook , Kim Hyun-Jin , Cheon Yun-Hong , Kim Mingyo , Lee Hanna , Kim Hyun-Ok , Lee Sang-Il
Published: June 2021

This study aimed to identify differences in clinical and dietary characteristics, serum adipokine levels, and metabolomic profiles between early- and late-onset gout. Eighty-three men with gout were divided into an early-onset group (n = 38, aged < 40 years) and a late-onset group (n = 45, aged ≥ 40 years). Dietary and clinical information was obtained at baseline. Serum adipokines, including adiponectin, resistin, leptin, and plasminogen activator inhibitor-1 (PAI-1), were quantified by a Luminex multiplex immunoassay. Metabolite expression levels in plasma were measured in 22 representative samples using metabolomics analysis based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Average body mass index, rate of consumption of sugar-sweetened beverages, and serum uric acid levels were significantly higher in the early-onset group (p < 0.05), as was the PAI-I concentration (105.01 ± 42.45 ng/mL vs. 83.76 ± 31.16 ng/mL, p = 0.013). Changes in levels of metabolites mostly involved those related to lipid metabolism. In the early-onset group, acylcarnitine analog and propylparaben levels were downregulated and negatively correlated with the PAI-1 concentration whereas LPC (22:6) and LPC (18:0) levels were upregulated and positively correlated with the PAI-1 concentration. Dietary and clinical features, serum adipokine concentrations, and metabolites differed according to whether the gout is early-onset or late-onset. The mechanisms of gout may differ between these groups and require different treatment approaches.
Dual-Emission Fluorescence Probe Based on CdTe Quantum Dots and Rhodamine B for Visual Detection of Mercury and Its Logic Gate Behavior
Authors: Gao Yuefeng , Xu Sai , Liu Zhijian , Yu Kezhen , Pan Xinxiang
Published: June 2021

It is urgent that a convenient and sensitive technique of detecting Hg2+ be developed because of its toxicity. Conventional fluorescence analysis works with a single fluorescence probe, and it often suffers from signal fluctuations which are influenced by external factors. In this research, a novel dual-emission probe assembled through utilizing CdTe quantum dots (QDs) and rhodamine B was designed to detect Hg2+ visually. Only the emission of CdTe QDs was quenched after adding Hg2+ in the dual-emission probe, which caused an intensity ratio change of the two different emission wavelengths and hence facilitated the visual detection of Hg2+. Compared to single emission QDs-based probe, a better linear relationship was shown between the variation of fluorescence intensity and the concentration of Hg2+, and the limit of detection (LOD) was found to be11.4 nM in the range of 0–2.6 μM. Interestingly, the intensity of the probe containing Hg2+ could be recovered in presence of glutathione (GSH) due to the stronger binding affinity of Hg2+ towards GSH than that towards CdTe QDs. Based on this phenomenon, an IMPLICATION logic gate using Hg2+/GSH as inputs and the fluorescence signal of QDs as an output was constructed.
A Review on Analytical Modeling for Collapse Mode Capacitive Micromachined Ultrasonic Transducer of the Collapse Voltage and the Static Membrane Deflections
Authors: Wang JiuJiang , Liu Xin , Yu YuanYu , Li Yao , Cheng ChingHsiang , Zhang Shuang , Mak PengUn , Vai MangI , Pun SioHang
Published: June 2021

Analytical modeling of capacitive micromachined ultrasonic transducer (CMUT) is one of the commonly used modeling methods and has the advantages of intuitive understanding of the physics of CMUTs and convergent when modeling of collapse mode CMUT. This review article summarizes analytical modeling of the collapse voltage and shows that the collapse voltage of a CMUT correlates with the effective gap height and the electrode area. There are analytical expressions for the collapse voltage. Modeling of the membrane deflections are characterized by governing equations from Timoshenko, von Kármán equations and the 2D plate equation, and solved by various methods such as Galerkin’s method and perturbation method. Analytical expressions from Timoshenko’s equation can be used for small deflections, while analytical expression from von Kármán equations can be used for both small and large deflections.
The Respiratory Commensal Bacterium Dolosigranulum pigrum 040417 Improves the Innate Immune Response to Streptococcus pneumoniae
Authors: Raya Tonetti Fernanda , Tomokiyo Mikado , Ortiz Moyano Ramiro , Quilodrán-Vega Sandra , Yamamuro Hikari , Kanmani Paulraj , Melnikov Vyacheslav , Kurata Shoichiro , Kitazawa Haruki , Villena Julio
Published: June 2021

Previously, we demonstrated that the nasal administration of Dolosigranulum pigrum 040417 differentially modulated the respiratory innate immune response triggered by the activation of Toll-like receptor 2 in infant mice. In this work, we aimed to evaluate the beneficial effects of D. pigrum 040417 in the context of Streptococcus pneumoniae infection and characterize the role of alveolar macrophages (AMs) in the immunomodulatory properties of this respiratory commensal bacterium. The nasal administration of D. pigrum 040417 to infant mice significantly increased their resistance to pneumococcal infection, differentially modulated respiratory cytokines production, and reduced lung injuries. These effects were associated to the ability of the 040417 strain to modulate AMs function. Depletion of AMs significantly reduced the capacity of the 040417 strain to improve both the reduction of pathogen loads and the protection against lung tissue damage. We also demonstrated that the immunomodulatory properties of D. pigrum are strain-specific, as D. pigrum 030918 was not able to modulate respiratory immunity or to increase the resistance of mice to an S. pneumoniae infection. These findings enhanced our knowledge regarding the immunological mechanisms involved in modulation of respiratory immunity induced by beneficial respiratory commensal bacteria and suggested that particular strains could be used as next-generation probiotics.