bluuebunny/crossref_metadata_2025 huggingface dataset:
Fabrication of flexible AlGaInP LED
Authors: Liu Qiaoli , Feng Yajie , Tian Huijun , He Xiaoying , Hu Anqi , Guo Xia
Published: March 2020

Flexible light-emitting diodes (LEDs) are highly desired for wearable devices, flexible displays, robotics, biomedicine, etc. Traditionally, the transfer process of an ultrathin wafer of about 10–30 μm to a flexible substrate is utilized. However, the yield is low, and it is not applicable to thick GaN LED chips with a 100 μm sapphire substrate. In this paper, transferable LED chips utilized the mature LED manufacture technique are developed, which possesses the advantage of high yield. The flexible LED array demonstrates good electrical and optical performance.
Analysis of the time domain characteristics of tapered semiconductor lasers
Authors: Zeng Desheng , Zhong Li , Liu Suping , Ma Xiaoyu
Published: March 2020

We use traveling wave coupling theory to investigate the time domain characteristics of tapered semiconductor lasers with DBR gratings. We analyze the influence of the length of second order gratings on the power and spectrum of output light, and optimizing the length of gratings, in order to reduce the mode competition effect in the device, and obtain the high power output light wave with good longitudinal mode characteristics.
Combining climate, economic, and social policy builds public support for climate action in the US
Authors: Bergquist Parrish , Mildenberger Matto , Stokes Leah C
Published: March 2020

Despite the gravity of the climate threat, governments around the world have struggled to pass and implement climate policies. Today, politicians and advocates are championing a new idea: linking climate policy to other economic and social reforms. Will this approach generate greater public support for climate action? Here, we test this coalition-building strategy. Using two conjoint experiments on a representative sample of 2,476 Americans, we evaluate the marginal impact of 40 different climate, social, and economic policies on support for climate reforms. Overall, we find climate policy bundles that include social and economic reforms such as affordable housing, a $15 minimum wage, or a job guarantee increase US public support for climate mitigation. Clean energy standards, regardless of which technologies are included, also make climate policy more popular. Linking climate policy to economic and social issues is particularly effective at expanding climate policy support among people of color.
Pickling behavior of 2205 duplex stainless steel hot-rolled strips in mixed solutions of HNO3-HF
Authors: Peng Jianguo , Yang Yong , Li Moucheng
Published: March 2020

The pickling behaviour of 2205 DSS hot-rolled strips after annealing and sand blasting was studied in the mixed solutions of HNO3-HF using weight loss rate, surface analysis, corrosion potential, and electrochemical impedance spectroscopy measurements. The pickling rate decreased as the concentration of HNO3 increased in the mixed solutions of x HNO3 + 25 g l−1 HF (x = 40 ∼ 200 g l−1) at 50 °C due to passivation. The passivating effect of HNO3 became weak as its concentration was less than 80 g l−1. The pickling rate increased noticeably when the concentration of HF in the mixed solutions of 120 g l−1 HNO3 + y HF (y = 5 ∼ 80 g l−1) at 50 °C was increased. The dissolution of the metal under the oxide scale may have changed from the passive state to the active state as the HF concentration was higher than 25 g l−1. The pickling dissolution accelerated noticeably as the temperature rose from 40 to 60 °C. With the optimised pickling process, 2205 DSS hot-rolled strips can be pickled industrially with good efficiency and surface topography.
Substitutional transition metal doping in MoS2: a first-principles study
Authors: Yoshimura Anthony , Koratkar Nikhil , Meunier Vincent
Published: March 2020

Single-layer MoS2 is a direct-gap semiconductor whose band edges character is dominated by the d-orbitals of the Mo atoms. It follows that substitutional doping of the Mo atoms has a significant impact on the material’s electronic properties, namely the size of the band gap and the position of the Fermi level. Here, density functional theory is used along with the G0W0 method to examine the effects of substituting Mo with four different transition metal dopants: Nb, Tc, Ta, and Re. Nb and Ta possess one less valence electron than Mo does and are therefore p-type dopants, while Re and Tc are n-type dopants, having one more valence electron than Mo has. Four types of substitutional structures are considered for each dopant species: isolated atoms, lines, three-atom clusters centered on a S atom (c3s), and three-atom clusters centered on a hole (c3h). The c3h structure is found to be the most stable configuration for all dopant species. However, electronic structure calculations reveal that isolated dopants are preferable for efficient n- or p-type performance. Lastly, it is shown that photoluminescence measurements can provide valuable insight into the atomic structure of the doped material. Understanding these properties of substitutionally-doped MoS2 can allow for its successful implementation into cutting-edge solid state devices.
A C/V2O5 core-sheath nanofibrous cathode with mixed-ion intercalation for aluminium-ion batteries
Authors: Canever Nicolò , Nann Thomas
Published: March 2020

A new nanofibrous material, consisting of a conductive carbon core and an external layer made of vanadium oxide, has been studied as a cathode for aluminium-ion batteries. The material enables a mixed-ion intercalation mechanism, resulting in the alternating insertion of Al3+ and

AlCl

4

−

in the

V

2

O

5

and carbon layers, respectively. This is a highly desirable feature for cathode materials which may increase the energy density of future batteries by optimising the utilisation of the electrolyte.
Serology Is More Sensitive Than Urea Breath Test or Stool Antigen for the Initial Diagnosis of Helicobacter pylori Gastritis When Compared With Histopathology
Authors: Bosch Dustin E , Krumm Niklas , Wener Mark H , Yeh Matthew M , Truong Camtu D , Reddi Deepti M , Liu Yongjun , Swanson Paul E , Schmidt Rodney A , Bryan Andrew
Published: March 2020

Objectives

To assess the concordance and performance characteristics of Helicobacter pylori laboratory tests compared with histopathology and to propose algorithms for the diagnosis of H pylori that minimize diagnostic error.

Methods

H pylori diagnostics were reviewed from a 12-year period within a health system (2,560 cases). Analyses were performed to adjust diagnostic performance based on treatment and consensus histopathologic diagnoses among pathologists. Markers of access to care, including test cancellation frequency and turnaround time, were assessed. Costs and performance of candidate noninvasive testing algorithms were modeled as a function of disease prevalence.

Results

Serum H pylori IgG demonstrated a higher sensitivity (0.94) than urea breath and stool antigen tests (0.64 and 0.61, respectively). Evidence of an advantage in access to care for serology included a lower cancellation rate. Interobserver variability was higher (κ = 0.34) among pathologists for cases with a discordant laboratory test than concordant cases (κ = 0.56). A model testing algorithm utilizing serology for first-time diagnoses minimizes diagnostic error.

Conclusions

Although H pylori serology has modestly lower specificity than other noninvasive tests, the superior sensitivity and negative predictive value in our population support its use as a noninvasive test to rule out H pylori infection. Reflexive testing with positive serology followed by either stool antigen or urea breath test may optimize diagnostic accuracy in low-prevalence populations.
Comparison of Tissue Molecular Biomarker Testing Turnaround Times and Concordance Between Standard of Care and the Biocartis Idylla Platform in Patients With Colorectal Cancer
Authors: Tsongalis Gregory J , Al Turkmani M Rabie , Suriawinata Michael , Babcock Michael J , Mitchell Kristi , Ding Yi , Scicchitano Lisa , Tira Adrian , Buckingham Lela , Atkinson Sara , Lax Amy , Aisner Dara L , Davies Kurtis D , Wood Holly N , O’Neill Stacey S , Levine Edward A , Sequeira Judy , Harada Shuko , DeFrank Gina , Paluri Ravikumar , Tan Bradford A , Colabella Heather , Snead Christopher , Cruz-Correa Marcia , Ramirez Virginia , Rojas Arnaldo , Huang Huiya , Mackinnon Alexander C , Garcia Fernando U , Cavone Sharon M , Elfahal Mutasim , Abel Gyorgy , Vasef Mohammad A , Judd Andrew , Linder Mark W , Alkhateeb Khaled , Skinner William L , Boccia Ralph , Patel Kashyap
Published: March 2020

Objectives

Management of colorectal cancer warrants mutational analysis of KRAS/NRAS when considering anti–epidermal growth factor receptor therapy and BRAF testing for prognostic stratification. In this multicenter study, we compared a fully integrated, cartridge-based system to standard-of-care assays used by participating laboratories.

Methods

Twenty laboratories enrolled 874 colorectal cancer cases between November 2017 and December 2018. Testing was performed on the Idylla automated system (Biocartis) using the KRAS and NRAS-BRAF cartridges (research use only) and results compared with in-house standard-of-care testing methods.

Results

There were sufficient data on 780 cases to measure turnaround time compared with standard assays. In-house polymerase chain reaction (PCR) had an average testing turnaround time of 5.6 days, send-out PCR of 22.5 days, in-house Sanger sequencing of 14.7 days, send-out Sanger of 17.8 days, in-house next-generation sequencing (NGS) of 12.5 days, and send-out NGS of 20.0 days. Standard testing had an average turnaround time of 11 days. Idylla average time to results was 4.9 days with a range of 0.4 to 13.5 days.

Conclusions

The described cartridge-based system offers rapid and reliable testing of clinically actionable mutation in colorectal cancer specimens directly from formalin-fixed, paraffin-embedded tissue sections. Its simplicity and ease of use compared with other molecular techniques make it suitable for routine clinical laboratory testing.
Automatic identification of relevant genes from low-dimensional embeddings of single-cell RNA-seq data
Authors: Angerer Philipp , Fischer David S , Theis Fabian J , Scialdone Antonio , Marr Carsten
Published: March 2020

Motivation

Dimensionality reduction is a key step in the analysis of single-cell RNA-sequencing data. It produces a low-dimensional embedding for visualization and as a calculation base for downstream analysis. Nonlinear techniques are most suitable to handle the intrinsic complexity of large, heterogeneous single-cell data. However, with no linear relation between gene and embedding coordinate, there is no way to extract the identity of genes driving any cell’s position in the low-dimensional embedding, making it difficult to characterize the underlying biological processes.

Results

In this article, we introduce the concepts of local and global gene relevance to compute an equivalent of principal component analysis loadings for non-linear low-dimensional embeddings. Global gene relevance identifies drivers of the overall embedding, while local gene relevance identifies those of a defined sub-region. We apply our method to single-cell RNA-seq datasets from different experimental protocols and to different low-dimensional embedding techniques. This shows our method’s versatility to identify key genes for a variety of biological processes.

Availability and implementation

To ensure reproducibility and ease of use, our method is released as part of destiny 3.0, a popular R package for building diffusion maps from single-cell transcriptomic data. It is readily available through Bioconductor.

Supplementary information

Supplementary data are available at Bioinformatics online.
Using Mendelian randomization to understand and develop treatments for neurodegenerative disease
Authors: Storm Catherine S , Kia Demis A , Almramhi Mona , Wood Nicholas W
Published: March 2020

Common neurodegenerative diseases are thought to arise from a combination of environmental and genetic exposures. Mendelian randomization is a powerful way to leverage existing genetic data to investigate causal relationships between risk factors and disease. In recent years, Mendelian randomization has gathered considerable traction in neurodegenerative disease research, providing valuable insights into the aetiology of these conditions. This review aims to evaluate the impact of Mendelian randomization studies on translational medicine for neurodegenerative diseases, highlighting the advances made and challenges faced. We will first describe the fundamental principles and limitations of Mendelian randomization and then discuss the lessons from Mendelian randomization studies of environmental risk factors for neurodegeneration. We will illustrate how Mendelian randomization projects have used novel resources to study molecular pathways of neurodegenerative disease and discuss the emerging role of Mendelian randomization in drug development. Finally, we will conclude with our view of the future of Mendelian randomization in these conditions, underscoring unanswered questions in this field.
The effects of sepsis on endothelium and clinical implications
Authors: Dolmatova Elena V , Wang Keke , Mandavilli Rohan , Griendling Kathy K
Published: March 2020

Sepsis accounts for nearly 700 000 deaths in Europe annually and is caused by an overwhelming host response to infection resulting in organ failure. The endothelium is an active contributor to sepsis and as such represents a major target for therapy. During sepsis, endothelial cells amplify the immune response and activate the coagulation system. They are both a target and source of inflammation and serve as a link between local and systemic immune responses. In response to cytokines produced by immune cells, the endothelium expresses adhesion molecules and produces vasoactive compounds, inflammatory cytokines, and chemoattractants, thus switching from an anticoagulant to procoagulant state. These responses contribute to local control of infection, but systemic activation can lead to microvascular thrombosis, capillary permeability, hypotension, tissue hypoxia, and ultimately tissue damage. This review focuses on the role of the endothelium in leucocyte adhesion and transmigration as well as production of reactive oxygen and nitrogen species, microRNAs and cytokines, formation of signalling microparticles, and disseminated intravascular coagulation. We also discuss alterations in endothelial permeability and apoptosis. Finally, we review the diagnostic potential of endothelial markers and endothelial pathways as therapeutic targets for this devastating disease.
Fitness consequences of divorce in the azure-winged magpie depends on the breeding experience of a new mate
Authors: Gao Li-Fang , Zhang Hai-Yang , Zhang Wen , Zhang Xiao-Dan , Zhu Zhen-Qin , Du Bo
Published: March 2020

Sexual conflict in producing and raising offspring is a critical issue in evolutionary ecology research. Individual experience affects their breeding performance, as measured by such traits of provisioning of offspring and engagement in extra-pair copulations, and may cause an imbalance in sexual conflict. Thus, divorce is hypothesized to occur within aged social pairs, irrespective of current reproductive success. This concept was explored in the azure-winged magpie Cyanopica cyanus by investigating the divorce of a social pair and its relationship to their changes in breeding performance with prior experience. Females engaging in extra-pair copulation may intensify sexual conflicts and may be the main reason for divorce. Once divorced, females repairing with an inexperienced male realized higher reproductive success than that repairing with an experienced male; males repairing with an experienced female realized higher reproductive success than that repairing with an inexperienced female. This finding indicates that the fitness consequence of divorce depends on the breeding experience of new mates. Divorced females can obtain more extra-pair copulations, whereas divorced males cannot, when they repair with inexperienced breeders. Divorced females provisioned a brood at lower rates than inexperienced females whereas divorced males had no such difference. It appears that divorced females can obtain an advantage in sexual conflicts with inexperienced mates in future reproduction. Consequently, females are probably more active than males in divorcing their aged mates so as to select an inexperienced male as a new mate. Azure-winged magpies thus provide novel insights into the implications of sexual conflict in birds.
Utility of major basic protein, eotaxin-3, and mast cell tryptase staining for prediction of response to topical steroid treatment in eosinophilic esophagitis: analysis of a randomized, double-blind, double dummy clinical trial
Authors: Dellon Evan S , Woosley John T , McGee Sarah J , Moist Susan E , Shaheen Nicholas J
Published: March 2020

Inflammatory factors in eosinophilic esophagitis (EoE), including major basic protein (MBP), eotaxin-3 (EOT3) and mast cell tryptase (TRP), may predict treatment response to topical corticosteroids (tCS). We aimed to determine whether baseline levels of these markers predict response to tCS for EoE. To do this, we analyzed data from a randomized trial comparing two topical steroids for treatment of newly diagnosed EoE (NCT02019758). A pretreatment esophageal biopsy was stained for MBP, EOT3, and TRP to quantify tissue biomarker levels (cells/mm2). Levels were compared between histologic responders (<15 eos/hpf) and nonresponders (the primary outcome), and endoscopic responders (EREFS<2) and nonresponders. Complete histologic response (<1 eos/hpf) was also assessed, and area under the receiver operator characteristic curve (AUC) was calculated. We also evaluated whether baseline staining predicted symptom relapse in the trial’s off-treatment observation phase. Baseline samples were evaluable in 110/111 subjects who completed the randomized trial. MBP levels were higher in nonresponders (n = 36) than responders (704 vs. 373 cells/mm2; P = 0.007), but EOT3 and TRP levels were not statistically different. The combination of all three stains had an AUC of 0.66 to predict response. For complete histologic response, baseline TRP levels were higher in nonresponders (n = 69) than responders (370 vs. 268 mast cells/mm2; P = 0.01), with an AUC of 0.65. The AUC for endoscopic response was 0.68. Baseline staining did not predict symptom recurrence after remission. Pretreatment MBP, EOT3, and TRP levels were not strongly or consistently associated with histologic or endoscopic response to topical steroids. While elevated TRP levels may be associated with nonresponse compared with complete response, the magnitude and predictive utilities were modest. Novel methods for predicting steroid response are still required.
Treatment protocol for secondary esophageal reconstruction using ‘supercharged’ colon interposition flaps
Authors: Esmonde N , Rodan W , Haisley K R , Joslyn N , Carboy J , Hunter J G , Schipper P H , Tieu B H , Hansen J , Dolan J P
Published: March 2020

Locoregional esophageal cancer is currently treated with induction chemoradiotherapy, followed by esophagectomy with reconstruction, using a gastric conduit. In cases of conduit failure, patients are temporized with a cervical esophagostomy and enteral nutrition until gastrointestinal continuity can be established. At our institution, we favor reconstruction, using a colon interposition with a ‘supercharged’ accessory vascular pedicle. Consequently, we sought to examine our technique and outcomes for esophageal reconstruction, using this approach. We performed a retrospective review of all patients who underwent esophagectomy at our center between 2008 and 2018. We identified those patients who had a failed gastric conduit and underwent secondary reconstruction. Patient demographics, perioperative details, and clinical outcomes were analyzed after our clinical care pathway was used to manage and prepare patients for a second major reconstructive surgery. Three hundred and eighty eight patients underwent esophagectomy and reconstruction with a gastric conduit. Seven patients (1.8%) suffered gastric conduit loss and underwent a secondary reconstruction using a colon interposition with a ‘supercharged’ vascular pedicle. Mean age was 70.1 (±7.3) years, and six patients were male. The transverse colon was used in four cases (57.1%), left colon in two cases (28.6%), and right colon in one case (14.3%). There were no deaths or loss of the colon interposition at follow-up. Three patients (42.9%) developed an anastomotic leak, which resolved with conservative management. All patients had resumption of oral intake within 30 days. Utilizing a ‘supercharging’ technique for colon interposition may improve the perfusion to the organ and may decrease morbidity. Secondary reconstruction should occur when the patient’s oncologic, physiologic, and psychosocial condition is optimized. Our outcomes and preoperative strategies may provide guidance for those centers treating this complicated patient population.
Insights into the community structure and lifestyle of the fungal root endophytes of tomato by combining amplicon sequencing and isolation approaches with phytohormone profiling
Authors: Manzotti Andrea , Bergna Alessandro , Burow Meike , Jørgensen Hans J L , Cernava Tomislav , Berg Gabriele , Collinge David B , Jensen Birgit
Published: March 2020

Little is known about the influence of host genotype and phytohormones on the composition of fungal endophytic communities. We investigated the influence of host genotype and phytohormones on the structure of the fungal endophytic communities of tomato roots by amplicon sequencing of the ITS1 region and combined this approach with isolation and functional characterization of the isolates. A significant effect of the host genotype on the dominant fungal species was found by comparing the cultivars Castlemart and UC82B and, surprisingly, root pathogens were among the most abundant taxa. In contrast, smaller changes in the relative abundance of the dominant species were found in mutants impaired in jasmonic acid biosynthesis (def1) and ethylene biosynthesis (8338) compared to the respective wild types. However, def1 showed significantly higher species richness compared to the wild type. Analysis of the phytohormone profiles of these genotypes indicates that changes in the phytohormone balance may contribute to this difference in species richness. Assessing the lifestyle of isolated fungi on tomato seedlings revealed the presence of both beneficial endophytes and latent pathogens in roots of asymptomatic plants, suggesting that the interactions between members of the microbiome maintain the equilibrium in the community preventing pathogens from causing disease.
Introducing a Face Sort Paradigm to Evaluate Age Differences in Emotion Perception
Authors: Hoemann Katie , Vicaria Ishabel M , Gendron Maria , Stanley Jennifer Tehan
Published: March 2020

Objectives

Previous research has uncovered age-related differences in emotion perception. To date, studies have relied heavily on forced-choice methods that stipulate possible responses. These constrained methods limit discovery of variation in emotion perception, which may be due to subtle differences in underlying concepts for emotion.

Method

We employed a face sort paradigm in which young (N = 42) and older adult (N = 43) participants were given 120 photographs portraying six target emotions (anger, disgust, fear, happiness, sadness, and neutral) and were instructed to create and label piles, such that individuals in each pile were feeling the same way.

Results

There were no age differences in number of piles created, nor in how well labels mapped onto the target emotion categories. However, older adults demonstrated lower consistency in sorting, such that fewer photographs in a given pile belonged to the same target emotion category. At the same time, older adults labeled piles using emotion words that were acquired later in development, and thus are considered more semantically complex.

Discussion

These findings partially support the hypothesis that older adults’ concepts for emotions and emotional expressions are more complex than those of young adults, demonstrate the utility of incorporating less constrained experimental methods into the investigation of age-related differences in emotion perception, and are consistent with existing evidence of increased cognitive and emotional complexity in adulthood.
Metabolomics hallmarks OPA1 variants correlating with their in vitro phenotype and predicting clinical severity
Authors: Chao de la Barca Juan Manuel , Fogazza Mario , Rugolo Michela , Chupin Stéphanie , Del Dotto Valentina , Ghelli Anna Maria , Carelli Valerio , Simard Gilles , Procaccio Vincent , Bonneau Dominique , Lenaers Guy , Reynier Pascal , Zanna Claudia
Published: March 2020

Interpretation of variants of uncertain significance is an actual major challenge. We addressed this question on a set of OPA1 missense variants responsible for variable severity of neurological impairments. We used targeted metabolomics to explore the different signatures of OPA1 variants expressed in Opa1 deleted mouse embryonic fibroblasts (Opa1−/− MEFs), grown under selective conditions. Multivariate analyses of data discriminated Opa1+/+ from Opa1−/− MEFs metabolic signatures and classified OPA1 variants according to their in vitro severity. Indeed, the mild p.I382M hypomorphic variant was segregating close to the wild-type allele, while the most severe p.R445H variant was close to Opa1−/− MEFs, and the p.D603H and p.G439V alleles, responsible for isolated and syndromic presentations, respectively, were intermediary between the p.I382M and the p.R445H variants. The most discriminant metabolic features were hydroxyproline, the spermine/spermidine ratio, amino acid pool and several phospholipids, emphasizing proteostasis, endoplasmic reticulum (ER) stress and phospholipid remodeling as the main mechanisms ranking OPA1 allele impacts on metabolism. These results demonstrate the high resolving power of metabolomics in hierarchizing OPA1 missense mutations by their in vitro severity, fitting clinical expressivity. This suggests that our methodological approach can be used to discriminate the pathological significance of variants in genes responsible for other rare metabolic diseases and may be instrumental to select possible compounds eligible for supplementation treatment.
Effects of climate and spawning stock structure on the spatial distribution of Northeast Arctic cod larvae
Authors: Endo Clarissa Akemi Kajiya , Vikebø Frode B , Yaragina Natalia A , Hjøllo Solfrid Sætre , Stige Leif Christian
Published: March 2020

The spatial distribution of fish early life stages can impact recruitment at later stages and affect population size and resilience. Northeast Arctic (NEA) cod spawning occurs along the Norwegian coast. Eggs, larvae, and pelagic juveniles drift near-surface towards the Barents Sea nursery area. In this study, a 35-year long time series of NEA cod larvae data was analysed in combination with factors that potentially may affect the distribution of eggs and larvae. These factors included biological aspects of the spawning stock, and environmental variables, such as water temperature, wind, ocean current, and prey abundance. Our aim was to shed light on how these factors influence larval abundance and distribution and how larval abundance and distribution influenced recruitment at age 3. We found that biomass and mean weight of the spawners were positively associated with larval abundance and that a high liver condition index of the spawners was associated with a north-easterly distribution of the larvae. The environmental variables showed generally weak or no correlations with abundance or distribution of larvae. Lastly, we found significant association between larval abundance and year-class abundance at age 3, while the spatial distribution metrics of the larvae, i.e. distribution extent, mean longitude, and mean latitude, showed no significant association with future year-class abundance.
Minimal Bubbling for Willmore Surfaces
Authors: Marque Nicolas
Published: March 2020

In this paper, we build an explicit example of a minimal bubble on a Willmore surface, showing there cannot be compactness for Willmore immersions of Willmore energy above $16 \pi $. Additionally, we prove an inequality on the 2nd residue for limits of sequences of Willmore immersions with simple minimal bubbles. Doing so, we exclude some gluing configurations and prove compactness for immersed Willmore tori of energy below $12 \pi $.
National monitoring and evaluation of eHealth: a scoping review
Authors: Villumsen Sidsel , Adler-Milstein Julia , Nøhr Christian
Published: March 2020

Objective

There has been substantial growth in eHealth over the past decade, driven by expectations of improved healthcare system performance. Despite substantial eHealth investment, little is known about the monitoring and evaluation strategies for gauging progress in eHealth availability and use. This scoping review aims to map the existing literature and depict the predominant approaches and methodological recommendations to national and regional monitoring and evaluation of eHealth availability and use, to advance national strategies for monitoring and evaluating eHealth.

Methods

Peer-reviewed and grey literature on monitoring and evaluation of eHealth availability and use published between January 1, 2009, and March 11, 2019, were eligible for inclusion. A total of 2354 publications were identified and 36 publications were included after full-text review. Data on publication type (eg, empirical research), country, level (national or regional), publication year, method (eg, survey), and domain (eg, provider-centric electronic record) were charted.

Results

The majority of publications monitored availability alone or applied a combination of availability and use measures. Surveys were the most common data collection method (used in 86% of the publications). Organization for Economic Co-operation and Development (OECD), European Commission, Canada Health Infoway, and World Health Organization (WHO) have developed comprehensive eHealth monitoring and evaluation methodology recommendations.

Discussion

Establishing continuous national eHealth monitoring and evaluation, based on international approaches and recommendations, could improve the ability for cross-country benchmarking and learning. This scoping review provides an overview of the predominant approaches to and recommendations for national and regional monitoring and evaluation of eHealth. It thereby provides a starting point for developing national eHealth monitoring strategies.
A Biodegradable Formulation of MCH (3-Methylcyclohex-2-en-1-one) for Protecting Pseudotsuga menziesii from Dendroctonus pseudotsugae (Coleoptera: Curculionidae) Colonization
Authors: Foote Gabriel G , Fettig Christopher J , Ross Darrell W , Runyon Justin B , Coleman Tom W , Gaylord Monica L , Graves Andrew D , McMillin Joel D , Mortenson Leif A , Mafra-Neto Agenor
Published: March 2020

Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, trees and stands can be protected from Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins (DFB)-caused mortality by application of synthetic formulations of the beetle’s antiaggregation pheromone, 3-methylcyclohex-2-en-1-one (MCH). A biodegradable formulation of MCH, SPLAT MCH, was developed and evaluated for protecting individual Douglas-fir trees and small stands from colonization and mortality by DFB. In an individual-tree experiment in Idaho, both MCH bubble capsules and SPLAT MCH significantly reduced the proportion of treated trees colonized and killed by DFB compared to untreated controls. SPLAT MCH was as effective as MCH bubble capsules for protecting individual trees. Both MCH bubble capsules and SPLAT MCH significantly reduced the proportion of trees colonized and killed by DFB within 0.04-ha circular plots surrounding each treated tree compared to untreated controls. In 0.41 ha stands in New Mexico, both MCH bubble capsules and SPLAT MCH significantly reduced the proportion of trees colonized and killed by DFB compared to untreated controls, again with no differences observed between MCH treatments. In a similar stand level trial in Idaho, neither MCH treatment significantly reduced the proportion of trees colonized by DFB, and only MCH bubble capsules significantly reduced levels of tree mortality compared to untreated controls, but no significant difference was observed between SPLAT MCH and MCH bubble capsules. Overall, the results indicate that SPLAT MCH is as effective as MCH bubble capsules for protecting individual trees and small stands of Douglas-fir from DFB-caused mortality.
Negative Life History Impacts for Habrobracon hebetor (Hymneoptera: Braconidae) that Develop in Bollworm Larvae Inoculated with Helicoverpa armigera Nucleopolyhedrovirus
Authors: Allahyari Rahim , Aramideh Shahram , Michaud J P , Safaralizadeh Mohammad Hassan , Rezapanah Mohammad Reza
Published: March 2020

Cotton bollworm, Helicoverpa armigera Hubner, is a cosmopolitan polyphagous pest of many crops. Habrobracon hebetor Say and Helicoverpa armigera nucleopolyhedrovirus (HearNPV) are two important biocontrol agents used to manage this pest, sometimes in combination. We evaluated the sublethal effects of HearNPV on H. hebetor life table parameters under laboratory conditions when its host (second instar H. armigera) was treated with HearNPV, and tested H. hebetor females for their ability to discriminate against inoculated hosts. Emergence of adults reared as solitary larvae was reduced by half on LC30-inoculated larvae compared to controls, but not on LC5 or LC15-inoculated hosts. Low concentrations (LC5, LC15, and LC30) of HearNPV had no effects on overall parasitoid developmental time, but longevity and lifetime fecundity was reduced for females emerging from hosts receiving the LC30 treatment. Net reproductive rate (R0), intrinsic rate of increase (rm), and finite rate of increase (λ) were all decreased in a concentration-dependent manner in the LC15 and LC30 treatments, as were female life expectancy, age-specific survivorship (lx), and age-specific fecundity (mx), whereas population doubling time (DT) increased. Parasitoids did not discriminate against LC15-inoculated larvae in choice or no-choice tests, but parasitized more LC50-inoculated hosts than controls in the choice test, with no significant differences in total numbers of eggs laid in either case. Although parasitoids suffered some loss of fitness in HearNPV-inoculated hosts under these laboratory conditions, these agents still appear compatible for joint application against H. armigera under field conditions, provided parasitoid releases are made 2 d after NPV application.
Integrative Transcriptome and Proteome Analyses Provide New Insights Into the Interaction Between Live Borrelia burgdorferi and Frontal Cortex Explants of the Rhesus Brain
Authors: Ding Zhe , Sun Luyun , Bi Yunfeng , Zhang Yu , Yue Peng , Xu Xin , Cao Wenjing , Luo Lisha , Chen Taigui , Li Lianbao , Ji Zhenhua , Jian Miaomiao , Lu Lihong , Abi Manzama-Esso , Liu Aihua , Bao Fukai
Published: March 2020

Borrelia burgdorferi (Bb), which is neurotropic, can attack the central nervous system (CNS), leading to the development of various neurologic symptoms. The pathogenesis of Lyme neuroborreliosis (LNB) remains poorly understood. Presently, there is a lack of knowledge of the changes in mRNA and proteins in the CNS following early disseminated Lyme disease. Explants from the frontal cortex of 3 rhesus brains were incubated with medium alone or with medium containing live Bb for 6, 12, or 24 hours. Then, we analyzed identified mRNA and proteins in the frontal cortex tissues, allowing for an in-depth view of the transcriptome and proteome for a macroscopic and unbiased understanding of early disseminated Lyme disease in the brain. Through bioinformatics analysis, a complex network of enriched pathways that were mobilized during the progression of Lyme spirochete infection was described. Furthermore, based on the analysis of omics data, translational regulation, glycosaminoglycan/proteoglycan-binding activity in colonization and dissemination to tissues, disease-associated genes, and synaptic function were enriched, which potentially play a role in pathogenesis during the interaction between frontal cortex tissues and spirochetes. These integrated omics results provide unbiased and comprehensive information for the further understanding of the molecular mechanisms of LNB.
Giant Appendicolith: A Case Report and Review of the Literature
Authors: Pahissa Robert A , Lin-Hurtubise Kevin M
Published: March 2020

Acute appendicitis is one of the most prevalent causes of an acute abdomen. Although the cause of appendicitis is not completely understood, the theory of luminal obstruction is a popular belief, with appendicoliths being a common etiology. While appendicoliths are quite common, giant appendicoliths >2 cm are rare. Although previous reports cite only two or three other occurrences of giant appendicoliths, we found at least 11 reported cases in the literature. We present a young male diagnosed preoperatively on computed tomography to have a large appendiceal mass of 2.2 cm. This case is presented for the rarity of giant appendicoliths along with a review of the literature.
Radiation forces constrain the FRB mechanism
Authors: Kumar Pawan , Lu Wenbin
Published: March 2020

We provide constraints on fast radio burst (FRB) models by careful considerations of radiation forces associated with these powerful transients. We find that the induced Compton scatterings of the coherent radiation by electrons/positrons accelerate particles to very large Lorentz factors (LFs) in and around the source of this radiation. This severely restricts those models for FRBs that invoke relativistic shocks and maser-type instabilities at distances less than about 1013 cm of the neutron star. Radiation travelling upstream, in these models, forces particles to move away from the shock with an LF larger than the LF of the shock front. This suspends the photon generation process after it has been operating for less than ∼0.1 ms (observer frame duration). We show that masers operating in shocks at distances larger than 1013 cm cannot simultaneously account for the burst duration of 1 ms or more and the observed ∼GHz frequencies of FRBs without requiring an excessive energy budget (>1046 erg); the energy is not calculated by imposing any efficiency consideration, or other details, for the maser mechanism, but is entirely the result of ensuring that particle acceleration by induced Compton forces upstream of the shock front does not choke off the maser process. For the source to operate more or less continuously for a few ms, it should be embedded in a strong magnetic field – cyclotron frequency ≫ wave frequency – so that radiation forces do not disperse the plasma and shut off the engine.
Two Cobalt Chelatase Subunits Can Be Generated from a Single chlD Gene via Programed Frameshifting
Authors: Antonov Ivan V
Published: March 2020

Magnesium chelatase chlIDH and cobalt chelatase cobNST enzymes are required for biosynthesis of (bacterio)chlorophyll and cobalamin (vitamin B12), respectively. Each enzyme consists of large, medium, and small subunits. Structural and primary sequence similarities indicate common evolutionary origin of the corresponding subunits. It has been reported earlier that some of vitamin B12 synthesizing organisms utilized unusual cobalt chelatase enzyme consisting of a large cobalt chelatase subunit (cobN) along with a medium (chlD) and a small (chlI) subunits of magnesium chelatase. In attempt to understand the nature of this phenomenon, we analyzed >1,200 diverse genomes of cobalamin and/or chlorophyll producing prokaryotes. We found that, surprisingly, genomes of many cobalamin producers contained cobN and chlD genes only; a small subunit gene was absent. Further on, we have discovered a diverse group of chlD genes with functional programed ribosomal frameshifting signals. Given a high similarity between the small subunit and the N-terminal part of the medium subunit, we proposed that programed translational frameshifting may allow chlD mRNA to produce both subunits. Indeed, in genomes where genes for small subunits were absent, we observed statistically significant enrichment of programed frameshifting signals in chlD genes. Interestingly, the details of the frameshifting mechanisms producing small and medium subunits from a single chlD gene could be prokaryotic taxa specific. All over, this programed frameshifting phenomenon was observed to be highly conserved and present in both bacteria and archaea.
Killing Time Again
Authors: Vihvelin Kadri
Published: March 2020

I have argued that even if time travel is metaphysically possible, there are some things a time traveler would not be able to do. I reply here to critics who have argued that my account entails fatalism about the past or entails that the time traveler is unfree or that she is bound by “strange shackles.” My argument does not entail any sort of fatalism. The time traveler is able to do many of the things that everyone else can do and is as free as any non-time-traveler. The time traveler is constrained only as we all are by the laws of nature. My argument shows only how strangely those constraints must operate if those laws permit time travel.
Health-related quality of life and symptoms of conservative care versus dialysis in patients with end-stage kidney disease: a systematic review
Authors: Verberne Wouter R , van den Wittenboer Iris D , Voorend Carlijn G N , Abrahams Alferso C , van Buren Marjolijn , Dekker Friedo W , van Jaarsveld Brigit C , van Loon Ismay N , Mooijaart Simon P , Ocak Gurbey , van Delden Johannes J M , Bos Willem Jan W
Published: March 2020

Background

Non-dialytic conservative care (CC) has been proposed as a viable alternative to maintenance dialysis for selected older patients to treat end-stage kidney disease (ESKD). This systematic review compares both treatment pathways on health-related quality of life (HRQoL) and symptoms, which are major outcomes for patients and clinicians when deciding on preferred treatment.

Methods

We searched PubMed, Embase, Cochrane Library, Cumulative Index to Nursing and Allied Health Literature (CINAHL) Plus and PsycINFO from inception to 1 October 2019 for studies comparing patient-reported HRQoL outcomes or symptoms between patients who chose either CC or dialysis for ESKD.

Results

Eleven observational cohort studies were identified comprising 1718 patients overall. There were no randomized controlled trials. Studies were susceptible to selection bias and confounding. In most studies, patients who chose CC were older and had more comorbidities and worse functional status than patients who chose dialysis. Results were broadly consistent across studies, despite considerable clinical and methodological heterogeneity. Patient-reported physical health outcomes and symptoms appeared to be worse in patients who chose CC compared with patients who chose dialysis but had not yet started, but similar compared with patients on dialysis. Mental health outcomes were similar between patients who chose CC or dialysis, including before and after dialysis start. In patients who chose dialysis, the burden of kidney disease and impact on daily life increased after dialysis start.

Conclusions

The available data, while heterogeneous, suggest that in selected older patients, CC has the potential to achieve similar HRQoL and symptoms compared with a dialysis pathway. High-quality prospective studies are needed to confirm these provisional findings.
Macrophage interactions with collecting duct epithelial cells are capable of driving tubulointerstitial inflammation and fibrosis in immunoglobulin A nephropathy
Authors: Pawluczyk Izabella Z A , Soares Maria S F , Barratt William A , Brown Jeremy R , Bhachu Jasraj S , Selvaskandan Haresh , Zeng Yiqing , Sarania Rishi , Molyneux Karen , Roberts Ian S D , Barratt Jonathan
Published: March 2020

Background

Tubulointerstitial fibrosis is a powerful predictor of future progression inimmunoglobulin A (IgA) nephropathy (IgAN). Proximal tubular epithelial cells (PTECs), in concert with infiltrating macrophages, are regarded as the agents provocateurs for driving this fibrotic process. However, evidence is now emerging for a contributory role of the distal nephron. The aim of this study was to examine the potential influence of macrophages on collecting duct epithelial cells (CDECs) and their combined role in the progression of IgAN.

Methods

CDECs were cultured with macrophage-conditioned media (MCM) generated from human monocyte cell lines U937 and THP-1 stimulated with or without 100 μg/mL galactose-deficient IgA1. CDECs were analysed for evidence of inflammation and fibrosis.

Results

Staining of IgAN biopsies for CD68+ macrophages revealed the presence of macrophages juxtaposed to collecting ducts and within their lumina. CDEC exposed to MCM from IgA1-stimulated THP-1 cells (THP-1-IgA-MCM) exhibited markedly increased expression of neutrophil-associated gelatinase (NGAL) and proinflammatory cytokinesinterleukin (IL)-1β, tumour necrosis factor-α, IL-6 and IL-8 compared with MCM from non-IgA-stimulated THP-1 cells (THP-1-MCM). U937-IgA-MCM increased fibronectin levels and reduced E-cadherinmRNA expression. THP-1-IgA-MCM-derived exosomes induced similar increases in NGAL and cytokine expression while in cross-over experiments exosomes extracted from IL-1β-exposed CDEC induced IL-1β and IL-6 mRNA expression in both sets of macrophages. MiRnome analysis revealed that microRNA (miR)-146a, -155 and -200b exhibited a >2-fold increase in expression in CDEC treated with THP-1-IgA-MCM compared with THP-1-MCM. Enforced miR-146a suppression further enhanced NGAL expression, while ectopic miR-146a over-expression downregulated it. NGAL mRNA and miR-146a were upregulated in the biopsies of patients with progressive IgAN compared with non-progressive IgAN.

Conclusions

Taken together, these data suggest that CDEC–macrophage interactions potentially contribute to the tubulointerstitial fibrosis characteristic of progressive IgAN.