bluuebunny/crossref_metadata_2025 huggingface dataset:
Vae-Clip: Unveiling Deception through Cross-Modal Models and Multi-Feature Integration in Multi-Modal Fake News Detection
Authors: Zhou Yufeng , Pang Aiping , Yu Guang
Published: July 2024

With the development of internet technology, fake news has become a multi-modal collection. The current news detection methods cannot fully extract semantic information between modalities and ignore the rumor properties of fake news, making it difficult to achieve good results. To address the problem of the accurate identification of multi-modal fake news, we propose the Vae-Clip multi-modal fake news detection model. The model uses the Clip pre-trained model to jointly extract semantic features of image and text information using text information as the supervisory signal, solving the problem of semantic interaction across modalities. Moreover, considering the rumor attributes of fake news, we propose to fuse semantic features with rumor style features using multi-feature fusion to improve the generalization performance of the model. We use a variational autoencoder to extract rumor style features and combine semantic features and rumor features using an attention mechanism to detect fake news. Numerous experiments were conducted on four datasets primarily composed of Weibo and Twitter, and the results show that the proposed model can accurately identify fake news and is suitable for news detection in complex scenarios, with the highest accuracy reaching 96.3%.
A Survey on Neuromorphic Architectures for Running Artificial Intelligence Algorithms
Authors: Al Abdul Wahid Seham , Asad Arghavan , Mohammadi Farah
Published: July 2024

Neuromorphic computing, a brain-inspired non-Von Neumann computing system, addresses the challenges posed by the Moore’s law memory wall phenomenon. It has the capability to enhance performance while maintaining power efficiency. Neuromorphic chip architecture requirements vary depending on the application and optimising it for large-scale applications remains a challenge. Neuromorphic chips are programmed using spiking neural networks which provide them with important properties such as parallelism, asynchronism, and on-device learning. Widely used spiking neuron models include the Hodgkin–Huxley Model, Izhikevich model, integrate-and-fire model, and spike response model. Hardware implementation platforms of the chip follow three approaches: analogue, digital, or a combination of both. Each platform can be implemented using various memory topologies which interconnect with the learning mechanism. Current neuromorphic computing systems typically use the unsupervised learning spike timing-dependent plasticity algorithms. However, algorithms such as voltage-dependent synaptic plasticity have the potential to enhance performance. This review summarises the potential neuromorphic chip architecture specifications and highlights which applications they are suitable for.
Model-Free Temperature Control of Heat Treatment Process
Authors: Baciu Andrei , Lazar Corneliu
Published: July 2024

The heat treatment temperature must precisely meet the required profile to ensure the quality of the workpiece. The model of the heat treatment furnace is not precisely known because it is nonlinear and complex. Considering this aspect, model-free temperature control methods are proposed, which are not based on a model of the process, but only on the input and output data acquired from the control system. Since the controlled temperature must accurately track the reference formed by the ramp and soak signals in the presence of disturbances, model-free iPI controllers were chosen due to the included double-integral component that ensures the steady-state performance for ramp signals. For the tuning of the iPI controller parameters, the iterative feedback tuning method is proposed, and the performance is compared with that obtained using the sliding mode control iPI variant. The proposed iPI control laws are tested for ramp and soak scenarios, using temperature suitable to thermal treatment technological processes, and performance is analyzed using both process-specific indices and classical criteria.
Novel Approach to Analyzing Friction Losses by Modeling the Microflow of Lubricating Oil between the Piston Rings and Cylinder in Internal Combustion Engines
Authors: Wróblewski Piotr , Kachel Stanisław
Published: July 2024

This work focuses on the evolution of lubrication wedge shaping in internal combustion piston engines, taking into account liquid microflows on curved surfaces and coating microgeometries. It introduces a new approach to the analysis of friction losses by simulating the microflow of lubricating oil between the surfaces of piston rings cooperating with the cylinder surface. The models used take into account three types of microgeometry and material expansion. Key results indicate that microirregularities with a stereometry of 0.1–0.2 µm significantly influence the distribution of oil film thickness in the phase of maximum working pressure, which is critical for the functioning of the seal ring. The innovation of the work consists of demonstrating that, despite small changes in the friction force and power in the piston rings, changes in the minimum values of the oil film thickness are significant. The work highlights the failure to take into account microgeometry parameters in friction models, which leads to significant errors in the simulation results, especially in terms of oil film continuity and the contribution of mixed friction. The simulations also indicate that advanced geometric models with high mesh resolution are necessary only for the assessment of changes in oil film thickness during the highest pressure increase in the combustion chamber and taking into account various mixed friction conditions. The results suggest significant progress in engine design and performance, confirming the importance of advanced fluid and mixed friction models in piston engine lubrication research.
Research on Photovoltaic Power Generation Characteristics of Small Ocean Observation Unmanned Surface Vehicles
Authors: Yang Weiwei , Wang Bingzhen , Ke Wei , Shen Shuyuan , Wu Xiao
Published: July 2024

Under the action of waves, a small unmanned surface vehicle (USV) will experience continuous oscillation, significantly impacting its photovoltaic power generation system. This paper proposes a USV photovoltaic power generation simulation model, and the efficiency of photovoltaic MPPT control under wave action is studied. A simulation model for solar irradiance on solar panels of USV under wave action is established based on CFD and solar irradiation models. The dynamic changes in irradiance of USV solar panels under typical wave conditions are analyzed. The MPPT efficiency of USV photovoltaic power generation devices under continuously changing irradiance conditions is studied on this basis. The simulation research results indicate that waves and solar altitude angles significantly impact the instantaneous irradiation energy of USV photovoltaic devices. However, the impact of waves on the average irradiance is relatively tiny. The sustained oscillation of irradiance poses certain requirements for the Maximum Power Point Tracking (MPPT) control frequency of USV photovoltaic systems; a disturbance control frequency of no less than 50 Hz is proposed.
Black Tea Kombucha Consumption: Effect on Cardiometabolic Parameters and Diet Quality of Individuals with and without Obesity
Authors: Fraiz Gabriela Macedo , Costa Mirian A. C. , Cardoso Rodrigo R. , Hébert James R. , Zhao Longgang , Corich Viviana , Giacomini Alessio , Milagro Fermín I. , Barros Frederico A. R. , Bressan Josefina
Published: July 2024

Background: Kombucha, a fermented tea, has been suggested as an adjuvant in the treatment of obesity. Although animal and in vitro studies indicate its promising benefits, exploring kombucha’s impact on human health is necessary. Methods: This quasi-experimental pre–post-intervention assessed the effect of black tea kombucha consumption on cardiometabolic parameters for 8 weeks, considering the quality of the diet of individuals with and without obesity. Diet quality was assessed through the Dietary Inflammatory Index® and Dietary Total Antioxidant Capacity. Paired t-test/Wilcoxon was applied to compare differences between pre- and post-intervention (α = 0.05). Results: After the intervention, individuals with obesity showed a decrease in insulin, HOMA-IR, and GGT; those without obesity showed an increase in total cholesterol and alkaline phosphatase, but this was only observed in those with a worsened diet quality. Conclusion: kombucha intake demonstrated positive impacts on the metabolic health of individuals with obesity beyond the importance of combining it with healthy eating patterns.
Anticancer Potential of Valencia Peanut (Arachis hypogaea L.) Skin Extract against Cervical Cancer Cells In Vitro and in Nude Mouse Xenograft Models
Authors: Jeeunngoi Jarckrit , Senawong Gulsiri , Jogloy Sanun , Prompipak Jeerati , Samankul Arunta , Utaiwat Suppawit , Woranam Khanutsanan , Sripa Banchob , Senawong Thanaset
Published: July 2024

This study investigated the impact of Valencia KK4-type peanut skin ethanolic extract (KK4-PSE) combined with cisplatin or 5-fluorouracil (5-FU) on HeLa cells in vitro and in xenograft models. At exposure times of 24, 48 and 72 h, KK4-PSE inhibited the growth of HeLa cells with a half maximal inhibitory concentration (IC50) of 79.43 ± 0.54, 55.55 ± 1.57 and 41.32 ± 0.74 µg/mL, respectively. Drug interactions evaluated by the Chou–Talalay method demonstrated that KK4-PSE enhanced antiproliferative activity of 5-FU against HeLa cells with combination index (CI) values of 0.49 (48 h) and 0.60 (72 h), indicating a synergistic effect, while KK4-PSE combined with cisplatin exhibited an additive effect (CI = 1.02) at 72 h, and an antagonistic effect at 24 and 48 h exposures (CI = 1.12 and 1.18, respectively). In nude mouse xenograft models, the combination of 5-FU and KK4-PSE markedly reduced HeLa tumor weights compared with the control and single agent treatments groups. The combination of KK4-PSE and 5-FU achieved greater tumor growth inhibition than that of the KK4-PSE–cisplatin combination. KK4-PSE mitigated hepatotoxicity induced by both cisplatin and 5-FU in nude mice. The spleen hyaloserositis was significantly reduced in the combination treatment of 5-FU and KK4-PSE. These results suggest that KK4-PSE has the potential to limit cervical cancer cell proliferation while reducing the toxicity of cisplatin and 5-FU.
Atmospheric Cold Plasma as an Alternative to Chlorination in Soft Wheat Flour to Prepare High-Ratio Cakes
Authors: Mahanta Shikhadri , Bock Jayne , Mense Andrew , Kirk-Bradley Nahndi , Awika Joseph , Moore Janie McClurkin
Published: July 2024

Chlorination is a common chemical modification process of soft wheat flour to prepare high-ratio cakes. Due to safety and labeling concerns of flour chlorination, alternatives to chlorination have been researched. Atmospheric Cold Plasma (ACP) is an emerging technology which is applicable for a wide range of food and biological components, including cereal grain products. The potential of ACP as an alternative to chlorination for high-ratio cakes has not been researched. Soft wheat flour was treated at 50 kV, 60 kV, and 70 kV each for 5, 6, and 7 min and compared to untreated and chlorinated wheat flour. High-ratio cakes were prepared from the chlorinated, treated, and untreated soft wheat flour and their properties were compared. Changes in the flour properties and the high-ratio cakes were observed at different treatment conditions. It was found that after 50 kV, 6 min, 50 kV, 7 min and 60 kV, 6 min had the better flour pasting properties, higher cake volume, and better texture properties as compared to untreated wheat flour and chlorinated wheat flour. This determines the potential of the application of ACP as an alternative to chlorination or to reduce the use of chlorination in soft wheat flour.
Preparation, Characterization and Stability of Calcium-Binding Peptides Derived from Chicken Blood
Authors: Yang Jing , Shi Jing , Zhou Ying , Zou Ye , Xu Weimin , Xia Xiudong , Wang Daoying
Published: July 2024

Calcium-binding peptides have gained significant attention due to their potential applications in various fields. In this study, we aimed to prepare, characterize, and evaluate the stability of calcium-binding peptides derived from chicken blood. Chicken hemoglobin peptides (CPs) were obtained by protease hydrolysis and were applied to prepare chicken hemoglobin peptide–calcium chelate (CP-Ca). The preparation conditions were optimized, and the characteristics and stability of CP-Ca were analyzed. The optimal chelating conditions were determined by single-factor and response surface tests, and the maximum calcium ion chelating rate was 77.54%. Amino acid analysis indicated that glutamic acid and aspartic acid motifs played an important role in the chelation of the calcium ions and CP. According to the characterization analysis, CP-Ca was a different substance compared with CP; calcium ions chelated CPs via the sites of carbonyl oxygen, carboxyl oxygen, and amino nitrogen groups; and after the chelation, the structure changed from a smooth homogeneous plate to compact granular. The stability analysis showed that CP-Ca was stable at different temperatures, pH, and gastrointestinal conditions. The study indicates that chicken blood is a promising source of peptide–calcium chelates, providing a theoretical basis for application in functional foods and improving the utilization value of chicken blood.
Cereal-Derived Water-Unextractable Arabinoxylans: Structure Feature, Effects on Baking Products and Human Health
Authors: Huang Manchun , Bai Juan , Buccato Daniele Giuseppe , Zhang Jiayan , He Yufeng , Zhu Ying , Yang Zihan , Xiao Xiang , Daglia Maria
Published: July 2024

Arabinoxylans (AXs) are non-starch polysaccharides with complex structures naturally occurring in grains (i.e., barley, corn, and others), providing many health benefits, especially as prebiotics. AXs can be classified as water-extractable (WEAX) and water-unextractable (WUAX) based on their solubility, with properties influenced by grain sources and extraction methods. Numerous studies show that AXs exert an important health impact, including glucose and lipid metabolism regulation and immune system enhancement, which is induced by the interactions between AXs and the gut microbiota. Recent research underscores the dependence of AX physiological effects on structure, advocating for a deeper understanding of structure-activity relationships. While systematic studies on WEAX are prevalent, knowledge gaps persist regarding WUAX, despite its higher grain abundance. Thus, this review reports recent data on WUAX structural properties (chemical structure, branching, and MW) in cereals under different treatments. It discusses WUAX applications in baking and the benefits deriving from gut fermentation.
Morphological Features of Mathematical and Real-World Fractals: A Survey
Authors: Patiño-Ortiz Miguel , Patiño-Ortiz Julián , Martínez-Cruz Miguel Ángel , Esquivel-Patiño Fernando René , Balankin Alexander S.
Published: July 2024

The aim of this review paper is to survey the fractal morphology of scale-invariant patterns. We are particularly focusing on the scale and conformal invariance, as well as on the fractal non-uniformity (multifractality), inhomogeneity (lacunarity), and anisotropy (succolarity). We argue that these features can be properly quantified by the following six adimensional numbers: the fractal (e.g., similarity, box-counting, or Assouad) dimension, conformal dimension, degree of multifractal non-uniformity, coefficient of multifractal asymmetry, index of lacunarity, and index of fractal anisotropy. The difference between morphological properties of mathematical and real-world fractals is especially outlined in this review paper.
Antibacterial Hydrogels for Wound Dressing Applications: Current Status, Progress, Challenges, and Trends
Authors: Zhu Jie , Cheng Hongju , Zhang Zixian , Chen Kaikai , Zhang Qinchen , Zhang Chen , Gao Weihong , Zheng Yuansheng
Published: July 2024

Bacterial infection treatment for chronic wounds has posed a major medical threat and challenge. Bacteria at the wounded sites can compete with the immune system and subsequently invade live tissues, leading to more severe tissue damage. Therefore, there is an urgent demand for wound dressings with antibacterial and anti-inflammatory properties. Considering the concept of moist healing, hydrogels with a three-dimensional (3D) network structure are widely used as wound dressings due to their excellent hydrophilicity, water retention properties, and biocompatibility. Developing antibacterial hydrogels for the treatment of infected wounds has been receiving extensive attention recently. This article categorizes antibacterial hydrogels according to their materials and antibacterial modes, and introduces the recent findings and progress regarding their status. More importantly, with the development of emerging technologies, new therapies are utilized to prepare antibacterial hydrogels such as nanoenzymes, photothermal therapy (PTT), photodynamic therapy (PDT), metal–organic frameworks (MOFs), and other external stimuli-responsive methods. Therefore, this review also examines their progress, challenges, and future trends as wound dressings. In the following studies, there will still be a focus on antibacterial hydrogels that have a high performance, multi-functions, and intelligence, especially biocompatibility, a high and long-lasting antibacterial property, responsiveness, and on-demand therapeutic ability.
Development of Chitosan Polysaccharide-Based Magnetic Gel for Direct Red 83:1 Removal from Water
Authors: Murcia-Salvador Ainoa , Rodríguez-López María Isabel , Pellicer José Antonio , Gómez-Morte Teresa , Auñón-Calles David , Yáñez-Gascón María Josefa , Cerón-Carrasco José Pedro , Gil-Izquierdo Ángel , Núñez-Delicado Estrella , Gabaldón José Antonio
Published: July 2024

Water pollution caused by dyes is a significant environmental issue, necessitating the development of effective, cost-efficient decolorization methods suitable for industrial use. In this study, a Chitosan-Fe polymeric gel was synthesized, characterized, and tested for removing the azo dye Direct Red 83:1 from water. The polymeric magnetic chitosan was analyzed using various techniques: Field Emission Scanning Electron Microscopy (FE-SEM) revealed a porous structure, Differential Scanning Calorimetry (DSC) and Thermal Gravimetric Analysis (TGA) demonstrated the thermal stability, Infrared Spectrophotometry (IR) indicated the successful coordination of iron at the C3 position, and X-ray Powder Diffraction (XRD) confirmed the crystalline nature of the polymeric structure. Optimal conditions for kinetic and isotherm models were found at 1 g and pH 7.0. Adsorption behavior of Direct Red 83:1 onto magnetic chitosan gel beads was studied through kinetic tests and isotherm curves. The maximum adsorption capacity was 17.46 mg/g (qmax). The adsorption process followed pseudo-second-order kinetics (R2 = 0.999) and fit the Temkin isotherm (R2 = 0.946), suggesting heterogeneous surface adsorption. The newly synthesized Chitosan-Fe polymeric gel demonstrated good adsorption properties and facilitated easy separation of purified water.
Remote Sensing Technologies for Monitoring Argane Forest Stands: A Comprehensive Review
Authors: Mouafik Mohamed , Chakhchar Abdelghani , Fouad Mounir , El Aboudi Ahmed
Published: July 2024

This comprehensive review explores the ecological significance of the Argane stands (Argania spinosa) in southwestern Morocco and the pivotal role of remote sensing technology in monitoring forest ecosystems. Argane stands, known for their resilience in semi-arid and arid conditions, serve as a keystone species, preventing soil erosion, maintaining ecological balance, and providing habitat and sustenance to diverse wildlife species. Additionally, they produce an extremely valuable Argane oil, offering economic opportunities and cultural significance to local communities. Remote sensing tools, including satellite imagery, LiDAR, drones, radar, and GPS precision, have revolutionized our capacity to remotely gather data on forest health, cover, and responses to environmental changes. These technologies provide precise insights into canopy structure, density, and individual tree health, enabling assessments of Argane stand populations and detection of abiotic stresses, biodiversity, and conservation evaluations. Furthermore, remote sensing plays a crucial role in monitoring vegetation health, productivity, and drought stress, contributing to sustainable land management practices. This review underscores the transformative impact of remote sensing in safeguarding forest ecosystems, particularly the Argane forest stands, and highlights its potential for continued advancements in ecological research and conservation efforts.
Investigating Obstetric Violence in Ecuador: A Cross-Sectional Study Spanning the Last Several Years
Authors: Fors Martha , Falcón Kirsten , Brandao Thais , López Maria , Mena-Tudela Desirée
Published: July 2024

This pilot cross-sectional study was designed to determine the profile of obstetric violence in Ecuador in recent years. An online survey was conducted between March 2022 and April 2022, including women over 18 years who granted their informed consent to participate (n = 1598). We used non-probabilistic sampling to obtain our sample. Fisher’s exact test was performed to assess the association between violence and type of birth, healthcare facility, and education level. Out of the women who participated in the study, 89.2% (n = 1426) identified themselves as Mestiza. Additionally, 88.3% (n = 1411) had completed university-level education. The majority of the participants, specifically 63.6% (n = 1017), received their care in public institutions, and 98.2% (n = 1569) reported structural negligence, while 74.5% (n = 1190) reported violation of their right to information. The entire sample affirmed to have experienced violation of the right of presence. This report shows that obstetric violence is present in Ecuador in different ways and that women experience negligence and violation of their right to receive ethical healthcare during childbirth.
Emotional and Behavioural Problems in Spanish University Students: Association with Lifestyle Habits and Mental Well-Being
Authors: Tomás-Gallego Gloria , Jiménez Boraita Raúl , Ortuño Sierra Javier , Gargallo Ibort Esther , Dalmau Torres Josep María Dalmau
Published: July 2024

Emotional health represents a significant burden on the mental health of university students. Adapting to a new learning environment and facing increased academic pressure make this period particularly vulnerable for their emotional health and well-being. The objective of the study was to analyse the prevalence of emotional and behavioural problems in university students and their association with lifestyle habits, and mental and physical health indicators. A cross-sectional study was conducted on a sample of 1268 students (23.65 ± 7.84 years) from a university in northern Spain during November 2020 and March 2021. An online questionnaire was administered, comprising the self-report Strengths and Difficulties questionnaire, the Trait Meta-Mood Scale, the Rosenberg Self-Esteem Scale, the Satisfaction with Life Scale, the Perceived Stress Scale, the SENTIA-Brief Scale, the KIDMED questionnaire, the International Physical Activity Questionnaire-Short Form, the Alcohol Use Disorders Identification Test, and the Compulsive Internet Use Scale. 27.60% of students exhibited some form of emotional and behavioural problem. Students who did not present emotional and behavioural problems showed lower perceived stress, reduced suicidal behaviour and emotional intelligence deficits, as well as lower alcohol consumption and compulsive internet use (p < 0.001). Additionally, they reported higher engagement in physical activity and greater adherence to the Mediterranean diet (p < 0.001). The study shows that emotional and behavioural problems are recurring among university students, and given that modifiable psychosocial and lifestyle factors are associated with these issues, it underscores the need to develop multidisciplinary intervention strategies.
Exploring the Mediating Role of Self-Regulation in Bullying Victimization and Depressive Symptoms among Adolescents: A Cross-Regional and Gender Analysis
Authors: Huang Qi-Lu , Ho Wing-Shan , Cheung Ho-Nam
Published: July 2024

This study explores the mediating role of self-regulation in the relationship between bullying victimization and depressive symptoms among adolescents, considering the moderating effects of gender and region. A cross-sectional analysis was conducted with 3984 adolescents aged 12–18 from the United Kingdom, Hong Kong, Taiwan, and the Netherlands. Data were collected via an online survey administered through Qualtrics. The survey included validated measures such as the Illinois Bullying Scale (IBS) to measure bullying victimization, the Adolescent Self-Regulatory Inventory (ASRI) to measure self-regulation, and the Patient Health Questionnaire (PHQ) to measure depression. The SPSS macro PROCESS was employed for data analysis, with model 4 used for testing the mediating effects of self-regulation and model 1 for assessing the moderating effects of gender and region. The results demonstrated significant associations between bullying victimization, self-regulation, and depressive symptoms. Self-regulation mediated the positive association between bullying victimization and depression, with notable variations across genders and regions. Specifically, male students in Hong Kong exhibited an increased susceptibility to depression when subjected to bullying. These findings underscore the protective role of self-regulation in mitigating the adverse effects of bullying victimization on adolescent mental health. Implications for interventions and prevention strategies targeting adolescent depression are discussed.
Characterization of Bloom Iron Smelting Site Remains in Pržanj, Slovenia
Authors: Burja Jaka , Šetina Batič Barbara , Pavlovič Daša
Published: July 2024

This paper gives an overview of findings, connected with metallurgical activity, at the Pržanj archeological site near Ljubljana, Slovenia. More than 230 kg of slag and other remains connected with early medieval (from the 5th to the 12th century AD) metallurgical activities was found at the excavation site. The remains were grouped into four categories, i.e., furnace remains, ore, slag and a ferrous product, and analyzed in detail to obtain their chemical composition, microstructural characteristics, and mineral phase composition. The furnace wall remains, identified by their morphology and chemical composition, revealed an intensive iron processing activity at the site. The iron ore at the site was identified as goethite (FeO(OH)), a surprising find in Slovenia where limonite is typically used, and its presence suggests the potential exploitation of local bog iron ore, given the site’s geological context. Abundant slag remains at the site, identified by their shape, molten microstructure, and mineral components like wuestite, fayalite, and hercynite, indicated sophisticated smelting practices, including the use of CaO-rich materials to lower the melting temperature, a technique likely preserved from antiquity. Findings of ferrous products at ancient metallurgical sites are rare due to their value, but the discovery of a corroded iron bloom conglomerate at this site, initially mistaken for furnace remains, highlights the challenges in identifying small, corroded ferrous fragments that are often misidentified as ore. The results indicate extensive metallurgical activity at the excavation site, marking it as the first documented early medieval iron smelting production site in Slovenia.
Maximal Oxygen Consumption Is Negatively Associated with Fat Mass in Facioscapulohumeral Dystrophy
Authors: Crisafulli Oscar , Grattarola Luca , Bottoni Giorgio , Lacetera Jessica , Lavaselli Emanuela , Beretta-Piccoli Matteo , Tupler Rossella , Soldini Emiliano , D’Antona Giuseppe
Published: July 2024

Facioscapulohumeral dystrophy (FSHD) leads to progressive changes in body composition such as loss of muscle mass and increase in adiposity. In healthy subjects, anthropometric parameters are associated with the maximum volume of oxygen consumed per minute (VO2max), which is a health and function indicator in several populations of subjects, both healthy and pathological. Since VO2max can be difficult to test in patients with FSHD due to exercise intolerance, the identification of associated anthropometric parameters could provide new easily obtainable elements for the patients’ clinical stratification. The aim of this study was to evaluate whether anthropometric and body composition parameters are associated with VO2max in patients with FSHD. A total of 22 subjects with a molecular genetics-based diagnosis of FSHD (6 females, 16 males, mean age of 35.18 years) were recruited for the study. VO2max was measured by cardiopulmonary exercise tests (CPETs) on a cycle ergometer, utilizing a step incremental technique (15 Watts (W) every 30 s). Weight (Kg) and height (m) were obtained and utilized to calculate body mass index (BMI). Body composition parameters (fat mass (FM), fat free mass (FFM), and body cell mass (BCM)) were obtained by bioelectrical impedance analysis (BIA). Significant negative associations were found between VO2max and FM (Spearman correlation coefficient (SCC) −0.712), BMI (SCC −0.673), age (SCC −0.480), and weight (SCC −0.634), unlike FFM and BCM. Our results indicate that FM, BMI, age, and body weight are negatively associated with VO2max in patients with FSHD. This evidence may help practitioners to better stratify patients with FSHD.
Building Height Extraction Based on Spatial Clustering and a Random Forest Model
Authors: Chang Jingxin , Jiang Yonghua , Tan Meilin , Wang Yunming , Wei Shaodong
Published: July 2024

Building height (BH) estimation is crucial for urban spatial planning and development. BH estimation using digital surface model data involves obtaining ground and roof elevations. However, vegetation and shadows around buildings affect the selection of the required elevation, resulting in large BH estimation errors. In highly urbanized areas, buildings of similar heights often have similar characteristics and spatial proximity, which have reference significance in BH estimation but are rarely utilized. Herein, we propose a BH estimation method based on BIRCH clustering and a random forest (RF) model. We obtain the initial BH results using a method based on the optimal ground search area and a multi-index evaluation. BIRCH clustering and an RF classification model are used to match buildings of similar heights based on their spatial distance and attribute characteristics. Finally, the BH is adjusted based on the ground elevation obtained from the secondary screening and the BH matching. The validation results from two areas with over 12,000 buildings show that the proposed method reduces the root-mean-square error of the final BH results compared with the initial results. Comparing the obtained height maps shows that the final results produce a relatively accurate BH in areas with high shading and vegetation coverage, as well as in areas with dense buildings. Thus, the proposed method has been validated for its effectiveness and reliability.
Built Environment Effect on Metro Ridership in Metropolitan Area of Valparaíso, Chile, under Different Influence Area Approaches
Authors: Aprigliano Vicente , Seriani Sebastian , Toro Catalina , Rojas Gonzalo , Fukushi Mitsuyoshi , Cardoso Marcus , Silva Marcelino Aurelio Vieira da , Cucumides Cristo , de Oliveira Ualison Rébula , Henríquez Cristián , Braun Andreas , Hochschild Volker
Published: July 2024

The growing relevance of promoting a transition of urban mobility toward more sustainable modes of transport is leading to efforts to understand the effects of the built environment on the use of railway systems. In this direction, there are challenges regarding the creation of coherence between the locations of metro stations and their surroundings, which has been explored extensively in the academic community. This process is called Transit-Oriented Development (TOD). Within the context of Latin America, this study seeks to assess the influence of the built environment on the metro ridership in the metropolitan area of Valparaíso, Chile, testing two approaches of influence area definition, one of which is a fixed distance from the stations, and the other is based on the origin and destination survey of the study area. The analysis is based on Ordinary Least Squares regression (OLS) to identify the factors from the built environment, which affects the metro’s ridership. Results show that the models based on the area of influence defined through the use of the origin and destination survey explain the metro ridership better. Moreover, this study reveals that the metro system in Greater Valparaíso was not planned in harmony with urban development. The models demonstrate an inverse effect of the built environment on ridership, contrasting with the expected outcomes of a metro station designed following a Transit-Oriented Development approach.
ADAMTS13 in the New Era of TTP
Authors: Papakonstantinou Anna , Kalmoukos Panagiotis , Mpalaska Aikaterini , Koravou Evaggelia-Evdoxia , Gavriilaki Eleni
Published: July 2024

Thrombotic thrombocytopenic purpura (TTP) is a life-threatening, often immune-mediated disease that affects 2–13 persons per million per year. Hemolytic anemia, thrombocytopenia, and end-organ damage due to the formation of microthrombi are characteristic of TTP. ADAMTS13 is a disintegrin, metalloproteinase, cleaving protein of von Willebrand factor (VWF) that processes the VWF multimers to prevent them from interacting with platelets and, in turn, to microvascular thrombosis. Prompt diagnosis of TTP is critical yet challenging. Thrombotic microangiopathies have similar clinical presentation. Measurement of ADAMTS13 activity helps in the differential diagnosis. Less than 10% ADAMTS13 activity is indicative of TTP. Laboratory ADAMTS13 activity assays include incubating the test plasma with the substrate (full-length VWM multimers) and detection with direct or indirect measurement of the cleavage product. The purpose of this study is to examine the diagnostic potential, advantages, and weaknesses of the ADAMTS13 potency in TTP.
The Increase in the Frequency and Amplitude of the Beating of Isolated Mouse Tracheal Cilia Reactivated by ATP and cAMP with Elevation in pH
Authors: Kobayashi Akari , Kawaguchi Kotoku , Asano Shinji , Wu Hong , Nakano Takashi , Inui Toshio , Marunaka Yoshinori , Nakahari Takashi
Published: July 2024

Single cilia, 100 nm in diameter and 10 µm in length, were isolated from mouse tracheae with Triton X-100 (0.02%) treatment, and the effects of pH on ciliary beating were examined by measuring the ciliary beat frequency (CBF) and the ciliary bend distance (CBD—an index of amplitude) using a high-speed video microscope (250 fps). ATP (2.5 mM) plus 8Br-cAMP (10 µM) reactivated the CBF and CBD in the isolated cilia, similar to the cilia of in vivo tracheae. In the reactivated isolated cilia, an elevation in pH from 7.0 to 8.0 increased the CBF from 3 to 15 Hz and the CBD from 0.6 to 1.5 µm. The pH elevation also increased the velocity of the effective stroke; however, it did not increase the recovery stroke, and, moreover, it decreased the intervals between beats. This indicates that H+ (pHi) directly acts on the axonemal machinery to regulate CBF and CBD. In isolated cilia priorly treated with 1 µM PKI-amide (a PKA inhibitor), 8Br-cAMP did not increase the CBF or CBD in the ATP-stimulated isolated cilia. pH modulates the PKA signal, which enhances the axonemal beating generated by the ATP-activated inner and outer dyneins.
Threonine Deficiency Increases Triglyceride Deposition in Primary Duck Hepatocytes by Reducing STAT3 Phosphorylation
Authors: Zhuang Zhong , Jia Wenqian , Wu Lei , Li Yongpeng , Lu Yijia , Xu Minghong , Bai Hao , Bi Yulin , Wang Zhixiu , Chen Shihao , Jiang Yong , Chang Guobin
Published: July 2024

Liver lipid metabolism disruption significantly contributes to excessive fat buildup in waterfowl. Research suggests that the supplementation of Threonine (Thr) in the diet can improve liver lipid metabolism disorder, while Thr deficiency can lead to such metabolic disorders in the liver. The mechanisms through which Thr regulates lipid metabolism remain unclear. STAT3 (signal transducer and activator of transcription 3), a crucial transcription factor in the JAK-STAT (Janus kinase–signal transducer and activator of transcription) pathway, participates in various biological processes, including lipid and energy metabolism. This research investigates the potential involvement of STAT3 in the increased lipid storage seen in primary duck hepatocytes as a result of a lack of Thr. Using small interfering RNA and Stattic, a specific STAT3 phosphorylation inhibitor, we explored the impact of STAT3 expression patterns on Thr-regulated lipid synthesis metabolism in hepatocytes. Through transcriptome sequencing, we uncovered pathways related to lipid synthesis and metabolism jointly regulated by Thr and STAT3. The results showed that Thr deficiency increases lipid deposition in primary duck hepatocytes (p < 0.01). The decrease in protein and phosphorylation levels of STAT3 directly caused this deposition (p < 0.01). Transcriptomic analysis revealed that Thr deficiency and STAT3 knockdown jointly altered the mRNA expression levels of pathways related to long-chain fatty acid synthesis and energy metabolism (p < 0.05). Thr deficiency, through mediating STAT3 inactivation, upregulated ELOVL7, PPARG, MMP1, MMP13, and TIMP4 mRNA levels, and downregulated PTGS2 mRNA levels (p < 0.01). In summary, these results suggest that Thr deficiency promotes lipid synthesis, reduces lipid breakdown, and leads to lipid metabolism disorders and triglyceride deposition by downregulating STAT3 activity in primary duck hepatocytes.
The Aryl Hydrocarbon Receptor Regulates Invasiveness and Motility in Acute Myeloid Leukemia Cells through Expressional Regulation of Non-Muscle Myosin Heavy Chain IIA
Authors: Chang Fengjiao , Wang Lele , Kim Youngjoon , Kim Minkyoung , Lee Sunwoo , Lee Sang-woo
Published: July 2024

Acute myeloid leukemia (AML) is the most prevalent type of hematopoietic malignancy. Despite recent therapeutic advancements, the high relapse rate associated with extramedullary involvement remains a challenging issue. Moreover, therapeutic targets that regulate the extramedullary infiltration of AML cells are still not fully elucidated. The Aryl Hydrocarbon Receptor (AHR) is known to influence the progression and migration of solid tumors; however, its role in AML is largely unknown. This study explored the roles of AHR in the invasion and migration of AML cells. We found that suppressed expression of AHR target genes correlated with an elevated relapse rate in AML. Treatment with an AHR agonist on patient-derived AML cells significantly decreased genes associated with leukocyte trans-endothelial migration, cell adhesion, and regulation of the actin cytoskeleton. These results were further confirmed in THP-1 and U937 AML cell lines using AHR agonists (TCDD and FICZ) and inhibitors (SR1 and CH-223191). Treatment with AHR agonists significantly reduced Matrigel invasion, while inhibitors enhanced it, regardless of the Matrigel’s stiffness. AHR agonists significantly reduced the migration rate and chemokinesis of both cell lines, but AHR inhibitors enhanced them. Finally, we found that the activity of AHR and the expression of NMIIA are negatively correlated. These findings suggest that AHR activity regulates the invasiveness and motility of AML cells, making AHR a potential therapeutic target for preventing extramedullary infiltration in AML.
The Complete Mitogenome of Apostasia fujianica Y.Li & S.Lan and Comparative Analysis of Mitogenomes across Orchidaceae
Authors: Zheng Qinyao , Luo Xiaoting , Huang Ye , Ke Shi-Jie , Liu Zhong-Jian
Published: July 2024

Apostasia fujianica belongs to the genus Apostasia and is part of the basal lineage in the phylogenetic tree of the Orchidaceae. Currently, there are only ten reported complete mitochondrial genomes in orchids, which greatly hinders the understanding of mitochondrial evolution in Orchidaceae. Therefore, we assembled and annotated the mitochondrial genome of A. fujianica, which has a length of 573,612 bp and a GC content of 44.5%. We annotated a total of 44 genes, including 30 protein-coding genes, 12 tRNA genes, and two rRNA genes. We also performed relative synonymous codon usage (RSCU) analysis, repeat sequence analysis, intergenomic transfer (IGT) analysis, and Ka/Ks analysis for A. fujianica and conducted RNA editing site analysis on the mitochondrial genomes of eight orchid species. We found that most protein-coding genes are under purifying selection, but nad6 is under positive selection, with a Ka/Ks value of 1.35. During the IGT event in A. fujianica’s mitogenome, the trnN-GUU, trnD-GUC, trnW-CCA, trnP-UGG, and psaJ genes were identified as having transferred from the plastid to the mitochondrion. Compared to other monocots, the family Orchidaceae appears to have lost the rpl10, rpl14, sdh3, and sdh4 genes. Additionally, to further elucidate the evolutionary relationships among monocots, we constructed a phylogenetic tree based on the complete mitogenomes of monocots. Our study results provide valuable data on the mitogenome of A. fujianica and lay the groundwork for future research on genetic variation, evolutionary relationships, and breeding of Orchidaceae.
Comprehensive Review of COVID-19: Epidemiology, Pathogenesis, Advancement in Diagnostic and Detection Techniques, and Post-Pandemic Treatment Strategies
Authors: Chung Yiu-Sing , Lam Ching-Yin , Tan Pak-Hei , Tsang Hin-Fung , Wong Sze-Chuen Cesar
Published: July 2024

At present, COVID-19 remains a public health concern due to the ongoing evolution of SARS-CoV-2 and its prevalence in particular countries. This paper provides an updated overview of the epidemiology and pathogenesis of COVID-19, with a focus on the emergence of SARS-CoV-2 variants and the phenomenon known as ‘long COVID’. Meanwhile, diagnostic and detection advances will be mentioned. Though many inventions have been made to combat the COVID-19 pandemic, some outstanding ones include multiplex RT-PCR, which can be used for accurate diagnosis of SARS-CoV-2 infection. ELISA-based antigen tests also appear to be potential diagnostic tools to be available in the future. This paper also discusses current treatments, vaccination strategies, as well as emerging cell-based therapies for SARS-CoV-2 infection. The ongoing evolution of SARS-CoV-2 underscores the necessity for us to continuously update scientific understanding and treatments for it.
Serum Phosphatidylcholine Species 32:0 as a Biomarker for Liver Cirrhosis Pre- and Post-Hepatitis C Virus Clearance
Authors: Weigand Kilian , Peschel Georg , Grimm Jonathan , Höring Marcus , Krautbauer Sabrina , Liebisch Gerhard , Müller Martina , Buechler Christa
Published: July 2024

Phosphatidylcholine (PC) is an essential lipid for liver health and lipoprotein metabolism, but its circulating levels have rarely been studied in patients with cirrhosis. Chronic hepatitis C virus (HCV) infection causes lipid abnormalities and is a major cause of cirrhosis. Effective HCV elimination with direct-acting antivirals (DAAs) is associated with the normalization of serum low-density lipoprotein cholesterol levels. Since PC is abundant in all lipoprotein particles, this study analyzed the association between serum PC species levels and liver cirrhosis before and after HCV eradication. Therefore, 27 PC species were measured by Fourier Transform Mass Spectrometry in the serum of 178 patients with chronic HCV infection at baseline and in 176 of these patients at the end of therapy. The PC species did not correlate with viral load, and the levels of 13 PC species were reduced in patients infected with genotype 3a compared to those affected with genotype 1. Four PC species were slightly elevated 12 weeks after DAA initiation, and genotype-related changes were largely normalized. Patients with HCV and cirrhosis had higher serum levels of PC 30:0 and 32:0 before and at the end of therapy. PC species containing polyunsaturated fatty acids were mostly decreased in cirrhosis. The levels of polyunsaturated, but not saturated, PC species were inversely correlated with the model of the end-stage liver disease score. A receiver operating characteristic curve analysis showed area under the curve values of 0.814 and 0.826 for PC 32:0 and 0.917 and 0.914 for % PC 32:0 (relative to the total PC levels) for the classification of cirrhosis at baseline and at the end of therapy, respectively. In conclusion, the specific upregulation of PC 32:0 in cirrhosis before and after therapy may be of diagnostic value in HCV-related cirrhosis.
The Dual Faces of Oestrogen: The Impact of Exogenous Oestrogen on the Physiological and Pathophysiological Functions of Tissues and Organs
Authors: Bartkowiak-Wieczorek Joanna , Jaros Agnieszka , Gajdzińska Anna , Wojtyła-Buciora Paulina , Szymański Igor , Szymaniak Julian , Janusz Wojciech , Walczak Iga , Jonaszka Gabriela , Bienert Agnieszka
Published: July 2024

Oestrogen plays a crucial physiological role in both women and men. It regulates reproductive functions and maintains various non-reproductive tissues through its receptors, such as oestrogen receptor 1/oestrogen receptor α (ESR1/Erα), oestrogen receptor 2/oestrogen receptor β (ESR2/Erβ), and G protein-coupled oestrogen receptor 1 (GPER). This hormone is essential for the proper functioning of women’s ovaries and uterus. Oestrogen supports testicular function and spermatogenesis in men and contributes to bone density, cardiovascular health, and metabolic processes in both sexes. Nuclear receptors Er-α and Er-β belong to the group of transcription activators that stimulate cell proliferation. In the environment, compounds similar in structure to the oestrogens compete with endogenous hormones for binding sites to receptors and to disrupt homeostasis. The lack of balance in oestrogen levels can lead to infertility, cancer, immunological disorders, and other conditions. Exogenous endocrine-active compounds, such as bisphenol A (BPA), phthalates, and organic phosphoric acid esters, can disrupt signalling pathways responsible for cell division and apoptosis processes. The metabolism of oestrogen and its structurally similar compounds can produce carcinogenic substances. It can also stimulate the growth of cancer cells by regulating genes crucial for cell proliferation and cell cycle progression, with long-term elevated levels linked to hormone-dependent cancers such as breast cancer. Oestrogens can also affect markers of immunological activation and contribute to the development of autoimmune diseases. Hormone replacement therapy, oral contraception, in vitro fertilisation stimulation, and hormonal stimulation of transgender people can increase the risk of breast cancer. Cortisol, similar in structure to oestrogen, can serve as a biomarker associated with the risk of developing breast cancer. The aim of this review is to analyse the sources of oestrogens and their effects on the endogenous and exogenous process of homeostasis.