bluuebunny/crossref_metadata_2025 huggingface dataset:
O uso das tecnologias digitais no ensino-aprendizagem da língua inglesa no: ensino médio
Authors: Macedo Cleandro Farias , Carvalho Celso Batista
Published: September 2021

Esta pesquisa tem como objetivo geral analisar o uso de recursos tecnológicos nas práticas de ensino-aprendizagem de LE no primeiro ano do ensino médio na escola. Os objetivos específicos são observar as aulas de LI na escola, identificar os recursos tecnológicos usados em sala, descrever os recursos tecnológicos em uso e verificar as práticas de ensino-aprendizagem com uso dos recursos tecnológicos disponíveis na escola. Utilizou-se como base para a fundamentação teórica as teorias de aquisição de segunda língua MORAN (2012), na área de educação OLIVEIRA (2014) ensino-aprendizagem de LI, em linguística e Linguística Aplicada LEFFA (2007), VERA MENEZES (2008), para a área de metodologia do ensino teve como base TRIVIÑOS (1987), SOUZA (2009), que pelo caráter aberto e flexível, permite ao pesquisador transitar pelo cenário da pesquisa explorando questões que perpasse o discurso da investigação. Este trabalho é um estudo de campo qualitativo de cunho exploratório e os participantes desta pesquisa são o professor regente e os estudantes do ensino médio (EM) de uma determinada turma de uma escola pública de Manaus. Os instrumentos utilizados para gerar dados serão relatórios, questionário, entrevista com alunos e roda de conversas com professor regente e alunos durante o período de convivência na escola para o cumprimento do estágio supervisionado. Os resultados apontam o uso das novas tecnologias digitas como ferramentas pedagógicas no ensino de línguas estrangeiras LE, proporcionando aos estudantes o contato com a língua fora dos muros educacional.
Professional undergraduate internships as a work insertion device: work modality and hiring.
Authors: Sábala Martha , Rentería-Pérez Erico , Díaz-Bambula Fatima
Published: September 2021

The article discusses the need to reflect on the educational level related to changes and transformations in the world of work, especially regarding higher education's professional internships. Issues related to preparedness and the legitimation of social institutions are recognized, as well as the recognition of production and work systems. Those are referred to as devices for labor incorporation, as they become scenes to apply disciplinary theories and knowledge, in order to assume problems at real contexts, institutions, and organizations, with conditions associated with “decent or full jobs”. In this case, internships refer to a traditional status job, a concrete project-oriented to ensure a better future, (uncertain) working conditions, and to match with some kind of professional who works fiscally or virtually, collets data, is involved with technological structures, duplicates in networks, is flexible, handles with uncertainty, and works – as an identifiable trend- on precarious paradoxical condition of being a student-worker, characterized as well as a means for the actual re-institutionalization of the world of work as a form to be included and hired. This reflection is based on research experiences and conceptual approximation from academic and applied spaces at professional levels, related to concepts such as Employability in its multi-dimensional sense and includes labor insertion and recognizes the undergraduate professional internships phenomena as a working modality which is being consolidated at re re-institutionalization of the work itself, and ends up legitimating other social spheres of occupational activity that must be discussed beyond curricular and academic aspects exclusively. Finally, undergraduate professional internships are reconfigured as a social space of projection an insertion in and for work.
The new spacetime of telework: A challenge for the quality of worklife
Authors: Blanch Josep M.
Published: September 2021

Spatiotemporal coordinates are crucial for understanding work experience. The combined effect of the confinement in the face of Covid-19 andthe intense and accelerated development of digital technology changed the nature, forms, conditions, and working relationships. This transformation has been radical with regard to what has been modernly thought of as space and as working time. The objective of the study was to analyze psychosocial aspects of this new reality, visualizing its antecedents and reflecting on its implications. The information was collected from various types of sources: for the study of the antecedents, a selection of classic studies on work space-time was used, which provided a historiographic description of the paradigm inherited from modernity. For the perspective of the psychosocial implications of the new work environment, mainstream literature and secondary sources provided by the abundant multi-color literature available on Google were used. The collected material was subjected to a treatment inspired by thematic content analysis. In an initial phase, the core of the change was identified as a basically spatial phenomenon of distancing from the workplace. From this perspective, the focus of attention was the comparison of the advantages and disadvantages of face-to-face activity and interactions with virtual work, mediated by gadgets, clouds, networks, and virtual platforms. The psychosocial impact attributed to the combined digitization, internalization, teleworking from home, and virtualization of processes has been described as a dilution of the boundaries between work and non-work, as colonization and work contamination of domestic, family, private and personal space-time and, ultimately, as a threat, but also as an opportunity, for the quality of working life.
Do intellectual activity and manual work imply different links?
Authors: Borges Livia de Oliveira , Motta Georgina , Heleno Camila , Barros Sabrina
Published: September 2021

In the context of contrasting intellectual and manual work, we developed research to compare the turnover intentions from the organization among Human Resource Management (HRM) teachers and building construction workers. We applied the Behavioral Intentions to Stay in the Organization Scale (BISOS) and a sociodemographic form in 154 workers in Belo Horizonte, 77 teachers and 77 construction workers. We also applied semi-structured interviews to a small number of participants, exploring the participants’ arguments about these intentions. Among the teachers, there was a predominance of the intention to leave, whilst among the construction workers, the intention to stay. The difference between the distributions was statistically significant. We identified, however, a fragility of bonds with the organization for both occupational groups. On the one hand, the turnover intentions and the justifying arguments in their similarities and differences did not corroborate the expected polarization between intellectual and manual work. On the other hand, we can understand them as simultaneously reflecting the country’s current economic scenario and the educational profile of the two groups, in which construction workers have fewer options for reinsertion into the market, while teachers’ training enables other insertions into the market and/or having more than one job.
Lockdown 2.0 In Malaysia: Evaluating Forecast Performance of Goods Export with Box-Jenkins Methodology and ARIMA Model
Authors: Ramlan Muhammad Nadzif
Published: September 2021

The purpose of this study is to model the forecast of Malaysia's export of goods using Autoregressive Integrated Moving Average Model (ARIMA) modelling with Box-Jenkins method. The time-series concerned is from the first quarter of 2015 to the first quarter of 2021 based on the Department of Statistics Malaysia (DOSM) data. The empirical analysis focuses on the five criteria for consideration towards the best model: high significant coefficient, high adjusted R-squared value, low sigma squared value, low Akaike Information Criterion (AIC) and low Schwarz Information Criterion (SIC). The study showed that ARIMA (2,1,2) would be the best model to forecast Malaysian export of goods from the second quarter of 2021 to the fourth quarter of 2022. The quarterly forecast opined the performance rate of Malaysian goods export to be at a stable positive rate of 4.9% throughout 2022, indicating the economic recovery progress that Malaysia would acquire from its vaccination programme and Movement Control Order (MCO) done in the previous year. The annual forecast showed a more precise value after comparing the actual and forecast growth value of exports in 2021. This finding is further supported with qualitative analysis about the validity of the forecast values via reports released by sources such as World Bank and Focus Economics.
MEDIA PEMBELAJARAN INTERAKTIF UNTUK ANAK SEKOLAH MINGGU PADA GKE SINTA PARARAPAK
Authors: Nugroho Bayu Pratama , Hendartie Susi , Dwinanti Rosalia
Published: September 2021

Sunday School is a mentoring child's faith that is usually held on Sunday, this activity is to teach the basic Christian religion to the children of Sunday School with the delivery of material about Christianity according to the content of The Bible includes the introduction of Christian songs that are packed simple so it is easy to understand. But in the teaching and learning activities of Sunday School, the way teachers convey a message in the Bible is to tell. The conventional learning process and the limitations of props and take a long time to delivery the material.

In conducting this thesis research there are two methods used by the authors, which include methods of data collection and methods of system development. The data collection methods are observations, interviews, references, documentation, and questionnaires while the method of developing the author system is the development method of MDLC (Multimedia Development Life Cycle), and to design using UML (Uniefied Modelling Language).

The final result of this thesis study is the interactive learning media for the Sunday school children that can be used by children for Christian religious activities. Based on user's response to the interactive learning Media for Sunday school children is 80,4%.
VIRTUAL REALITY MUSEUM MULAWARMAN BERBASIS VIDEO 360°
Authors: Firdaus Muhammad Bambang , Tejawati Andi , Budiman Edy , Wahyudianto Mochamad Rizky , Anam M Khairul
Published: September 2021

Museum Mulawarman yang terletak di tenggarong Kutai Kartanegara beberapa tahun belakangan ini tampak mengalami penurunan pengunjung. Padahal di dalam museum banyak sekali informasi dan edukasi yang bisa di pelajari dan di dapatkan. Kurangnya media promosi bisa menjadi salah satu alasan berkurangnya pengunjung yang datang ke Museum Mulawarman. Penelitian ini bertujuan untuk menciptakan sebuah aplikasi video virtual tour pada Museum Mulawarman Tenggarong Kutai Kartanegara sebagai media promosi serta untuk memperkenalkan lebih jauh Museum Mulawarman . Tujuan penelitian tersebut dapat dicapai melalui penggabungan teknologi Video Virtual Reality (VR) dan teknologi mobile Android yang dimiliki hampir setiap lapisan masyarakat sehingga memungkinkan para pengguna aplikasi dapat melihat Video secara 360°. Hasil pengujian pada perangkat android menunjukan aplikasi yang dibuat dapat berjalan lancar dan cocok digunakan pada versi Android 5.1 keatas dengan aspek rasio layar 16:9 dan 18:9. Sementara hasil pengujian aplikasi berdasarkan kuisioner menggunakan purposive sampling dengan total 20 responden yang disebarkan menunjukan bahwa setiap pertanyaan dari kuisioner memperoleh jawaban setuju dan sangat setuju berkisar dari 85% hingga 100% yang mengindikasikan respon positif tentang aplikasi yang penulis rancang dari pengguna aplikasi.
Analysis of Smart Zone Heating in Different Heating Systems
Authors: Ingeli Rastislav , Buday Peter
Published: September 2021

The basic concept in the design of buildings with zero energy consumption is, in addition to high-quality thermal properties of the building envelope, also a correct and efficient system of heating and hot water preparation in residential buildings. One of the basic concepts when designing heating systems is a zone heating system. It is a system that brings effective regulation according to heating zones. In practice, the question sometimes arises as to whether zone regulation of individual rooms in small family houses is necessary. That is whether in such buildings, zone heating is not an unnecessary investment cost. In this paper, we analyze the effect of zone heating in two types of heat transfer systems on the internal operating temperature in the individual analyzed zones, which are interconnected by an internal partition structure. It is a verification that even in smaller spaces, zone heating has a significant potential for energy savings.
ONLINE FUNCTIONAL LITERACY, INTELLIGENT TUTORING SYSTEMS AND SCIENCE EDUCATION
Authors: Dolenc Kosta , Aberšek Boris , Kordigel Aberšek Metka
Published: September 2021

We live in a time of transition from print reading (off-line) to screen reading (on-line), where the role of the book and other literature is being taken over by different types of electronic devices (computers, tablets, smart phones). In the lives of young people, there is less and less printed media, because it is being pushed out by electronic media. Most written media that is still used is thus bound to the classroom. However, in recent years schools have also become more like e-schools. It is almost impossible to find a school that does not use e-material in its educational process. Research indicates that there are differences in reading comprehension when reading off-line and on-line. In a study in which 78 students from the 8th grade of elementary school participated at the course Technology and science (n=77; 53.2% female), it was shown that in order to overcome this difference, individualised and adaptive Intelligent Tutoring Systems (ITS) can be used. The evaluation of the results also indicates that, for such a form of ITS, there is still plenty of space for optimisation, which is a permanent method of improvement and upgrade in such systems. Key words: reading comprehension, Technology and science, ITS, elementary school.
EVALUATING THREE GRADING METHODS IN MIDDLE SCHOOL SCIENCE CLASSROOMS
Authors: Zhang Bo , Misiak Jacob
Published: September 2021

This study aims to fill a gap in middle school science education that no direct comparison has been conducted on three popular grading methods - point based, rubric based, and rubric plus written feedback based - on science achievement and motivation. Participants in this quasi-experimental study are 136 7th and 8th grade students from Midwest USA. Results indicate that for both grades, the written feedback group performs significantly better than the point-based and the rubric-only groups on both achievement and motivation measures. However, science achievement under rubric-based grading but without written feedback is not different from that under the traditional points-based grading, underscoring the important extra power that written feedback delivers. This study recommends that written feedback be highly relevant (to the academic standard being assessed), limited in number (to the major misconceptions students have), and giving a second chance (for students to act on to improve learning).
Key words: feedback, formative assessment, rubric, motivation, science achievement.
ENHANCING SCIENCE TEACHERS’ CONTINUOUS PROFESSIONAL DEVELOPMENT IN THE FIELD OF INQUIRY BASED SCIENCE EDUCATION
Authors: Kapanadze Marika , Bolte Claus , Schneider Vincent , Slovinsky Ekaterine
Published: September 2021

Decreasing motivation in science classes is often reported in many countries. Georgia, one of the post Soviet countries, has overcome the highly centralised educational system and nowadays implements a new national educational reform. National reforms demand more student-active and inquiry-based learning. Many Georgian teachers aren’t ready for these changes and need professional development training to enhance their professional skills in Inquiry-Based Science Education (IBSE). Therefore Ilia State University offers special continuous professional development (CPD) programs for science teachers in the frame of PROFILES project. The aim of the research was to determine what profession oriented attitudes and concerns Georgian in-service science teachers have regarding IBSE and what changes are visible after the implementation of PROFILES-based CPD programme. In order to conduct the research, the Stages of Concern model was used. 40 teachers took part in the two terms of the CPD programme. Research shows that the implemented CPD programme fosters the professional attitudes and concerns of Georgian science teachers in a positive manner. Therefore, it can be highly recommended to use the framework of the PROFILES-based CPD programme(s). Key words: IBSE, in-service teachers in Georgia, professional development, stages of concern.
The Effectiveness of Partial Lid Margin Excision Including Hair Follicles in Diffuse Trichiasis and Distichiasis
Authors: Shin In Choel , Kim Sang Duck
Published: September 2021

Purpose: In the present study, we introduced and evaluated the effectiveness of partial lid margin excision including hair follicles in diffuse trichiasis and distichiasis. Methods: A retrospective review of medical records was performed on 21 eyelids of 14 patients with diffuse trichiasis and distichiasis. The patients had trichiasis of more than 1/3 of the eyelid margin and received lid margin excision including hair follicles at our hospital. The patients were followed up for more than 3 months after surgical correction. A telephone survey of the surgical outcomes, including recurrence and cosmetic satisfaction, was conducted for patients who were unable to visit the clinic. Success was defined as complete resolution of symptoms, no recurrence of abnormal cilia, and acceptable cosmesis. Results: The patients included five males (6 eyes) and nine females (15 eyes) with an average age of 61.9 years (range, 39.8-82.4 years). The surgical success rate was 90.5%. Two eyelids showed a recurrence of trichiasis requiring additional electrolysis treatment, and one eyelid exhibited skin pigmentation. Conclusions: For patients with diffuse trichiasis and distichiasis, partial lid margin excision including hair follicles showed excellent results with respect to resolving irritating symptoms, with minimal complications, low recurrence, and an easy procedure.
The Effect of Scleral Sutured Intraocular Lens with Optic-haptic Junction Fixation in the Capsular Bag
Authors: Lee Sun Young , Cho Yang Kyung
Published: September 2021

Purpose: To compare the stability of intraocular lens (IOL) insertion methods of sutured haptics in the bag with scleral fixation to those of in the bag insertion and scleral sulcus fixation. Methods: In group of only phacodonesis, two IOL insertion methods, in the bag insertion and sutured haptics in the bag with scleral fixation were compared. In group of phacodonesis with less than 90° zonulysis, two IOL insertion methods, sutured haptics in the bag with scleral fixation and scleral sulcus fixation were compared. Postoperative 2 months and 12 months refraction were compared with target refraction and the refractive change between postoperative 2 and 12 months were compared in each group. The change of anterior chamber depth (ACD) at postoperative 2 and 12 months were compared with preoperative ACD, and the change of ACD between postoperative 2 and 12 months were compared in each group. Results: In group of only phacodonesis, the typical in the bag insertion group showed significant ACD change between postoperative 2 and 12 months (p = 0.010), but the group of sutured haptics in the bag with scleral fixation didn’t show the significant ACD change (p = 0.515). In group of phacodonesis with less than 90° zonulysis, the sulcus scleral fixation method showed significant ACD change and significant refractive change (p = 0.015, p = 0.004), but the group of sutured haptics in the bag with scleral fixation method didn’t show the significant ACD nor refractive change (p = 0.713, p = 0.106) between postoperative 2 and 12 months. Conclusions: In eyes with zonular instability, sutured haptics in the bag with scleral fixation method can promote the postoperative stability of IOL.
Treatment Results of Bevacizumab for Macular Edema Secondary to Branch Retinal Vein Occlusion
Authors: Choi Yu-Jin , Choi Young Je , Cho Yong Wun , Yoo Woong-Sun , Chung In Young
Published: September 2021

Purpose: To evaluate the results of treatment according to the method of intravitreal injection of bevacizumab for macular edema due to branch retinal vein occlusion (BRVO). Methods: The clinical records of macular edema patients were analyzed retrospectively for a total of 62 eyes of 62 patients who were injected with bevacizumab into the vitreous as the first treatment for BRVO. Best-corrected visual acuity (BCVA), the findings of spectral-domain optical coherence tomography before and after injection, and prognosis-related factors were evaluated for 21 eyes that received the initial three monthly loading treatments and the 41 eyes that did not. Results: Significant improvement in BCVA was observed in the group having received the initial three injections compared with the group who did not receive the injections at 3, 6, and 12 months (p = 0.025, p = 0.019, and p = 0.008, respectively). The central macular thickness (CMT) showed greater improvement in the initial three injections group than the group without at 6 months (p = 0.034). Multivariate regression showed that the duration from the onset, the three loadings, BCVA, disorganization of the retinal inner layer (DRIL), and choroidal thickness were predictors related to visual gain (p = 0.044, p = 0.047, p = 0.004, p = 0.045, and p = 0.034, respectively). Age, three loadings, BCVA, and DRIL were predictors related to final visual acuity (p = 0.045, p = 0.046, p = 0.002, and p = 0.034, respectively). Duration from the onset, CMT, and choroidal thickness were predictors related to CMT improvement (p = 0.042, p = 0.009, and p = 0.015, respectively). Conclusions: In macular edema of BRVO, the initial three monthly intravitreal injections of bevacizumab provided superior treatment outcomes regarding short-term functional and anatomical improvements and long-term functional improvement, compared with methods that did not treat with the initial three monthly injections.
Short-term Outcomes and Prognostic Factors of Cataract-combined Surgery with iStent versus iStent Inject
Authors: Gong Yeseul , Hwang Hyung Bin , Kang Kui Dong , Kim Yong Chan
Published: September 2021

Purpose: To analyze the therapeutic effects, prognostic factors, and complications that occurred after iStent® or iStent inject® implantation in South Korean glaucoma patients. Methods: We retrospectively analyzed 42 eyes of South Korean glaucoma patients, who were followed-up for more than 6 months after iStent® or iStent inject® implantation alone or combined with cataract surgery. Surgical success was defined as an intraocular pressure (IOP) ≤21 mmHg and an IOP reduction ≥20% from preoperative IOP levels. Results: The cumulative success rate at 6 months after surgery was 45.3 ± 8.1% for iStent inject® alone (group A), 28.6 ± 13.1% for iStent® alone (group B), 81.2 ± 6.8% for iStent inject® combined with cataract surgery (group A-1), and 27.3 ± 21.8% for iStent® combined with cataract surgery (group B-1). Group A-1 showed a higher success rate than group B-1 (p = 0.007); the difference in success rates between groups A and B was not statistically significant (p = 0.579). When the success prognostic factors were analyzed by Cox regression analysis, the results showed that a higher preoperative IOP was associated with a higher surgical success rate (hazard ratio 0.80, p = 0.02). Conclusions: In our patient group, iStent inject® was more effective than iStent® in combination with cataract surgery. The higher the preoperative IOP, the higher the surgical success rate; the results were significant.
Changes in the Glaucoma Diagnosis during the Last 10 years: Health Insurance Review and Assessment Service Data 2010-2019
Authors: Choi Ji Ho , Lee Tae Eun
Published: September 2021

Purpose: We determined the current status of glaucoma diagnoses in Korea and trends in the change over the past 10 years using data from the Health Insurance Review and Assessment Service. Methods: The glaucoma diagnostic code was collected from the disease subclass statistics of the medical statistical information disclosed in the Healthcare Bigdata open system, and the number of health insurance patients who were billed for medical care benefit costs from 2010 to 2019 with the glaucoma diagnosis code was collected. Data were collected for each diagnostic code by dividing it by year, age group, and gender, and then changes in the current status of glaucoma diagnosis per 100,000 people were analyzed by age group. A joinpoint regression analysis was used to determine the increasing and decreasing trends for each diagnostic code and whether the trend changed during the study period. Results: The number of health insurance patients who received treatment with the glaucoma diagnostic code in 2019 compared to 2010 increased by 120.3% and 109.5% per 100,000 people covered by medical care. This increasing trend has slowed as of 2015. Suspected glaucoma, primary open-angle glaucoma, and primary angle-closure glaucoma showed an increasing trend, while glaucoma secondary to eye trauma and glaucoma secondary to drugs declined markedly. No significant trend in glaucoma secondary to eye inflammation was detected, and glaucoma secondary to other eye disorders and unspecified glaucoma tended to increase. Other glaucoma diagnoses decreased from 2010 to 2012 and then increased. Conclusions: The number of patients who underwent glaucoma treatment among all health insurance patients increased significantly from 2010 to 2019. Despite the overall increasing trend, eye trauma and drug-induced secondary glaucoma have markedly decreased.
Small Cell Neuroendocrine Carcinoma of the Lacrimal Sac Presenting with Lacrimal Duct Obstruction
Authors: Choi Yeong A , Son Junhyuk
Published: September 2021

Purpose: We report a case of primary small cell neuroendocrine carcinoma arising from the lacrimal sac. Case summary: A 54-year-old man presented with epiphora that had been present for 1 month. He underwent lacrimal syringe analysis of the lower punctum in the right eye, which demonstrated fluid regurgitation from the upper punctum. Computed tomography revealed a 3.0 × 3.4 × 3.0 cm mass with an ill-defined margin in the right lacrimal sac, which demonstrated invasion of the lacrimal duct and bony destruction of the inferior and medial orbital walls. Incisional biopsy and histologic examination showed that the tumor was composed of small round tumor cells. On the basis of histopathological and immunohistochemical evaluations, a diagnosis of small cell neuroendocrine carcinoma was made. The patient received chemotherapy.Conclusions: To the best of our knowledge, this is the first case of primary small cell neuroendocrine carcinoma arising from the lacrimal sac in Korea. Small cell neuroendocrine carcinoma in the lacrimal sac should be regarded as a differential diagnosis for patients with a nasolacrimal duct obstruction.
Phakomatous Choristoma of the Orbit with Inferior Oblique Muscle Involvement
Authors: Heo Seo Yoon , Lee Haeng-Jin , Chung Myoung Ja , Ahn Min
Published: September 2021

Purpose: We report a case of phakomatous choristoma presenting as an orbital tumor with involvement of the inferior oblique muscle. Case summary: A 2-month-old male infant presented to our clinic with a right orbital mass that had been present since birth. Magnetic resonance imaging demonstrated a homogenous enhanced well-defined mass located in the inferomedial portion of the right orbit without bone erosion. By transconjunctival orbitotomy, the orbital tumor invading the inferior oblique muscle was identified and resected. Histopathology showed a thick basement membrane surrounding pseudoglandular structures embedded in a collagenous stroma, psammomatous calcific foci in the stroma, and eosinophilic material in the lumen. Immunohistochemical analysis showed positive staining for S-100 and cytokeratin. On histopathological evaluation, the tumor was diagnosed as phakomatous choristoma. Conclusions: To our knowledge, this is the first report in South Korea of phakomatous choristoma of the orbit with involvement of the inferior oblique muscle. Although rare, phakomatous choristoma should be included in the differential diagnosis of tumors occurring on the inferomedial side of the orbit.
Endophthalmitis Caused by Curtobacterium pusillum Following Open Globe Injury
Authors: Kim Sang Yoon , Kim Tae Hoon , Lee Sang Joon , Kwon Han Jo
Published: September 2021

Purpose: To report the first case of Curtobacterium endophthalmitis within 6 hours after open globe injury, with extensive phlebitis and secondary subretinal neovascularization. Case summary: A 53-year-old man with hypersensitivity to beta-lactam antibiotics was admitted due to visual disturbance in the left eye experienced while working in a rural area. Fundus examination was impossible due to a full-layer corneal laceration and traumatic cataract in the left eye. B-scan ultrasonography and orbital computed tomography showed no shadowing of retained intraocular foreign bodies. After a corneal scraping smear, primary closure, lensectomy, and vitrectomy were performed. Organic material was observed in a focal area of pale macula, accompanied by extensive retinal phlebitis in the mid-periphery. After diagnosing acute bacterial endophthalmitis, intravitreal vancomycin and dexamethasone were injected. Curtobacterium pusillum was cultured on an automated microbial identification system. Intravenous vancomycin and oral clarithromycin were administered for 2 weeks. After 3 months, endophthalmitis had not recurred, and the visual acuity reached 20/100. However, subretinal neovascularization was newly detected under the damaged macula. No complications of neovascularization were observed until 6 months after primary closure. Conclusions: Curtobacterium pusillum can induce acute endophthalmitis through direct penetration in cases of ocular trauma, and may be accompanied by extensive phlebitis and secondary subretinal neovascularization. In cases of open globe injury sustained in rural areas, acute endophthalmitis caused by a rare Gram-positive bacillus, such as Curtobacterium species, should be considered.
Efectos de la violencia sobre las tasas de emigración interna municipal en México: 1995-2015
Authors: Rodríguez Chávez Oscar
Published: September 2021

This article explores the relationship between the increase of violence in Mexico and the rising level of internal municipal emigration before, during, and after the so-called Mexican Drug War, which started in 2007. Through a linear regression and multinomial logistic models, it is shown that violence has had a positive and significant effect on the increase of internal emigration rates, particularly in municipalities with the highest internal emigration rates during the 2005-2010 period. In addition, the effect of violence tendsto be greater on female emigration rates compared to males. This indicates the increase of forced internal displacement in Mexico due to violence in recent years. However, more studies are needed to shed light on forced displacement and the effects of high violence levels on internal and international migration.
Poéticas pandêmicas no corredor de vozes: os discursos da apropriação e da reciclagem
Authors: Alfeld Elisabete
Published: September 2021

O tempo de pandemia foi (e ainda é) o novo tempo, o tempo da vida remota dependente das telas que, sob a variedade de formatos e gêneros, gerou a profusão de novos conteúdos e novos procedimentos discursivos contaminados pela avassaladora ação de um vírus. Neste cenário, o trânsito entre as matérias jornalísticas e as manifestações artísticas esteve presente em outdoors, vídeos, performances e instalações. O mundo se reorganizou e, nesse processo, foram significativas as narrativas sobre esse “novo mundo”. Assim, é diante da diversidade de discursos, de contextos comunicativos e de suportes que se localiza o objeto de nosso estudo: interessa-nos abordar nas poéticas pandêmicas os procedimentos de apropriação e reciclagem presentes nas práticas discursivas de algumas das manifestações da produção artística.
Morte, ritos fúnebres e luto na pandemia de Covid-19 no Brasil
Authors: Silva Andreia Vicente da , Rodrigues Claudia , Aisengart Rachel
Published: September 2021

Desde março de 2020, a pandemia do novo coronavírus acarretou impactos nos ritos de morte e na vivência do luto no Brasil. Com a publicação do “Manual de manejo dos corpos” pelo Ministério da Saúde, expressões ritualizadas da morte foram abreviadas e certas fases do rito suprimidas. Neste artigo apontamos características dos ritos funerários e argumentamos que a interferência no ciclo ritual acarreta desdobramentos para os enlutados e a coletividade, sendo mais intensos quanto maior o distanciamento do modelo vigente no contexto urbano. A interferência nos conteúdos e fases dos rituais voltados aos mortos pode lançar os sobreviventes em um processo de luto desordenado, gerando a possibilidade de repercussões futuras.
Guarani, Kaingang e Xetá: o curso de pedagogia para os povos indígenas/bilíngue da Unicentro na terra indígena Rio das Cobras em tempos de pandemia
Authors: Domingos Vanessa Toledo , Santos Rodrigo dos , Collares Solange Aparecida de Oliveira
Published: September 2021

Este artigo apresenta uma discussão em torno de como o Curso de Pedagogia para os Povos Indígenas da Universidade Estadual do Centro-Oeste (Unicentro), organizado de forma bilíngue, desenvolveu-se durante a pandemia de Covid-19. Para desenvolver esse objetivo, situamos o leitor na constituição do Curso e de como ele se estrutura pensando a língua materna de três etnias: Guarani, Kaingang e Xetá; e o português no seu currículo. Empregamos uma análise com pesquisadores que trabalharam com essa temática, promovendo uma discussão bibliográfica e documental. Como resultados, percebemos que, apesar dos processos terem se desenvolvido de maneira satisfatória, há ainda muito a avançar nos debates sobre ensino remoto emergencial, principalmente para os povos indígenas.
Doença na recepção do mito de Filoctetes no Regime Militar brasileiro: “Ramom, o Filoteto Americano” de Carlos Henrique Escobar
Authors: Dagios Mateus
Published: September 2021

No cruzamento entre história e teatro, o artigo analisa a peça “Ramom, o Filoteto Americano” (1975), de Carlos Henrique Escobar, escrita durante a Ditadura Militar brasileira e laureada no Concurso Nacional de Dramaturgia de 1975. Parte-se do conceito de recepção da antiguidade para entender como são utilizados elementos do mito grego em um contexto de repressão. O autor reelabora os principais elementos da tragédia “Filoctetes” de Sófocles para construir uma sensibilidade voltada às angústias que emergiam em um dos períodos mais repressores da Ditadura. O artigo demonstra como Ramom é um drama crítico que ressignifica a doença de Filoctetes. No corpo torturado de Ramom ecoam as mazelas de uma sociedade repressiva e colonizada. O artigo atenta para as possibilidades de investigação em uma peça complexa que aproveita a herança grega para explorar temas sensíveis da Ditadura e que pela radicalidade performática e ousadia não despertou a atenção dos censores.
The Dynamic Changes of Transcription Factors During the Development Processes of Human Biparental and Uniparental Embryos
Authors: Zhang Chenxi , Li Conghui , Yang Ling , Leng Lizhi , Jovic Dragomirka , Wang Jun , Fang Fang , Li Guibo , Zhao Depeng , Li Xuemei , Lin Lin , Luo Yonglun , Bolund Lars , Huang Jinrong , Lin Ge , Xu Fengping
Published: September 2021

Previous studies have revealed that transcription factors (TFs) play important roles in biparental (BI) early human embryogenesis. However, the contribution of TFs during early uniparental embryo development is still largely unknown. Here we systematically studied the expression profiles of transcription factors in early embryonic development and revealed the dynamic changes of TFs in human biparental and uniparental embryogenesis by single-cell RNA sequencing (scRNA-seq). In general, the TF expression model of uniparental embryos showed a high degree of conformity with biparental embryos. The detailed network analysis of three different types of embryos identified that 10 out of 17 hub TFs were shared or specifically owned, such as ZNF480, ZNF581, PHB, and POU5F1, were four shared TFs, ZFN534, GTF3A, ZNF771, TEAD4, and LIN28A, were androgenic (AG) specific TFs, and ZFP42 was the only one parthenogenetic (PG) specific TF. All the four shared TFs were validated using human embryonic stem cell (hESC) differentiation experiments; most of their target genes are responsible for stem cell maintenance and differentiation. We also found that Zf-C2H2, HMG, and MYB were three dominant transcription factor families that appeared in early embryogenesis. Altogether, our work provides a comprehensive regulatory framework and better understanding of TF function in human biparental and uniparental embryogenesis.
Ion Mobility Coupled to a Time-of-Flight Mass Analyzer Combined With Fragment Intensity Predictions Improves Identification of Classical Bioactive Peptides and Small Open Reading Frame-Encoded Peptides
Authors: Peeters Marlies K. R. , Baggerman Geert , Gabriels Ralf , Pepermans Elise , Menschaert Gerben , Boonen Kurt
Published: September 2021

Bioactive peptides exhibit key roles in a wide variety of complex processes, such as regulation of body weight, learning, aging, and innate immune response. Next to the classical bioactive peptides, emerging from larger precursor proteins by specific proteolytic processing, a new class of peptides originating from small open reading frames (sORFs) have been recognized as important biological regulators. But their intrinsic properties, specific expression pattern and location on presumed non-coding regions have hindered the full characterization of the repertoire of bioactive peptides, despite their predominant role in various pathways. Although the development of peptidomics has offered the opportunity to study these peptides in vivo, it remains challenging to identify the full peptidome as the lack of cleavage enzyme specification and large search space complicates conventional database search approaches. In this study, we introduce a proteogenomics methodology using a new type of mass spectrometry instrument and the implementation of machine learning tools toward improved identification of potential bioactive peptides in the mouse brain. The application of trapped ion mobility spectrometry (tims) coupled to a time-of-flight mass analyzer (TOF) offers improved sensitivity, an enhanced peptide coverage, reduction in chemical noise and the reduced occurrence of chimeric spectra. Subsequent machine learning tools MS2PIP, predicting fragment ion intensities and DeepLC, predicting retention times, improve the database searching based on a large and comprehensive custom database containing both sORFs and alternative ORFs. Finally, the identification of peptides is further enhanced by applying the post-processing semi-supervised learning tool Percolator. Applying this workflow, the first peptidomics workflow combined with spectral intensity and retention time predictions, we identified a total of 167 predicted sORF-encoded peptides, of which 48 originating from presumed non-coding locations, next to 401 peptides from known neuropeptide precursors, linked to 66 annotated bioactive neuropeptides from within 22 different families. Additional PEAKS analysis expanded the pool of SEPs on presumed non-coding locations to 84, while an additional 204 peptides completed the list of peptides from neuropeptide precursors. Altogether, this study provides insights into a new robust pipeline that fuses technological advancements from different fields ensuring an improved coverage of the neuropeptidome in the mouse brain.
Metabolites Profiling of Melanoma Interstitial Fluids Reveals Uridine Diphosphate as Potent Immune Modulator Capable of Limiting Tumor Growth
Authors: Vecchio Eleonora , Caiazza Carmen , Mimmi Selena , Avagliano Angelica , Iaccino Enrico , Brusco Teresa , Nisticò Nancy , Maisano Domenico , Aloisio Annamaria , Quinto Ileana , Renna Maurizio , Divisato Giuseppina , Romano Simona , Tufano Martina , D’Agostino Massimo , Vigliar Elena , Iaccarino Antonino , Mignogna Chiara , Andreozzi Francesco , Mannino Gaia Chiara , Spiga Rosangela , Stornaiuolo Mariano , Arcucci Alessandro , Mallardo Massimo , Fiume Giuseppe
Published: September 2021

Tumor interstitial fluid (TIF) surrounds and perfuses tumors and collects ions, metabolites, proteins, and extracellular vesicles secreted by tumor and stromal cells. Specific metabolites, accumulated within the TIF, could induce metabolic alterations of immune cells and shape the tumor microenvironment. We deployed a metabolomic approach to analyze the composition of melanoma TIF and compared it to the plasma of C57BL6 mice, engrafted or not with B16-melanoma cells. Among the classes of metabolites analyzed, monophosphate and diphosphate nucleotides resulted enriched in TIF compared to plasma samples. The analysis of the effects exerted by guanosine diphosphate (GDP) and uridine diphosphate (UDP) on immune response revealed that GDP and UDP increased the percentage of CD4+CD25+FoxP3–and, on isolated CD4+T-cells, induced the phosphorylation of ERK, STAT1, and STAT3; increased the activity of NF-κB subunits p65, p50, RelB, and p52; increased the expression of Th1/Th17 markers including IFNγ, IL17, T-bet, and RORγt; and reduced the expression of IL13, a Th2 marker. Finally, we observed that local administrations of UDP in B16-engrafted C57BL6 mice reduced tumor growth and necrotic areas. In addition, UDP-treated tumors showed a higher presence of MHCIIhitumor-associated macrophage (TAM) and of CD3+CD8+and CD3+CD4+tumor-infiltrating T-lymphocytes (TILs), both markers of anti-tumor immune response. Consistent with this, intra-tumoral gene expression analysis revealed in UDP-treated tumors an increase in the expression of genes functionally linked to anti-tumor immune response. Our analysis revealed an important metabolite acting as mediator of immune response, which could potentially represent an additional tool to be used as an adjuvant in cancer immunotherapy.
Comprehensive Analysis of the Prognostic Significance of Hsa-miR-100-5p and Its Related Gene Signature in Stomach Adenocarcinoma
Authors: Wang Gaoming , Yang Ludi , Hu Miao , Hu Renhao , Wang Yongkun , Chen Bo , Jiang Xiaohua , Cui Ran
Published: September 2021

Stomach adenocarcinoma (STAD) is one of the most common cancers in the world. However, the prognosis of STAD remains poor, and the therapeutic effect of chemotherapy and immunotherapy varies from person to person. MicroRNAs (miRNAs) play vital roles in tumor development and metastasis and can be used for cancer diagnosis and prognosis. In this study, hsa-miR-100-5p was identified as the only dysregulated miRNA in STAD samples through an analysis of three miRNA expression matrices. A weighted gene co-expression network analysis (WGCNA) was performed to select hsa-miR-100-5p-related genes. A least absolute shrinkage and selection operator (LASSO) Cox regression analysis was performed to establish a miR-100-5p-related prognostic signature. Kaplan–Meier analyses, nomograms, and univariate and multivariate Cox regression analyses were used to evaluate the prognostic signature, which was subsequently identified as an independent risk factor for STAD patients. We investigated the tumor immune environment between low- and high-risk groups and found that, among component types, M2 macrophages contributed the most to the difference between these groups. A drug sensitivity analysis suggested that patients with high-risk scores may be more sensitive to docetaxel and cisplatin chemotherapy and that patients in the low-risk group may be more likely to benefit from immunotherapy. Finally, external cohorts were evaluated to validate the robustness of the prognostic signature. In summary, this study may provide new ideas for developing more individualized therapeutic strategies for STAD patients.
One-Step Synthesis of Green Fluorescent Carbon Dots for Chloride Detecting and for Bioimaging
Authors: Yue Juan , Yu Ling , Li Li , Liu Pai , Mei Qian , Dong Wen-Fei , Yang Ru
Published: September 2021

The chloride ion is an essential ion in organisms, which plays an important role in maintaining normal cell functions. It is involved in many cell activities, such as cell proliferation, cell excitability regulation, immune response, and volume regulation. Accurate detection of the chloride ion can balance its concentration in vivo, which is of great significance. In this study, we developed a green fluorescent carbon quantum dot to detect chloride concentration through the “off–on” mechanism. First, the fluorescence of carbon dots is quenched by the complex of sulfhydryl and silver ions on the surface of carbon dots. Then, the addition of chloride ions pulls away the silver ions and restores the fluorescence. The fluorescence recovery is linearly related to the concentration of chloride ions, and the limit of detection is 2.817 μM, which is much lower than those of other reported chloride probes. Besides, cell and zebrafish experiments confirmed the biosafety and biocompatibility of the carbon dots, which provided a possibility for further applications in bioimaging in vivo.
Optimizing Species Richness in Mosaic Landscapes: A Probabilistic Model of Species-Area Relationships
Authors: Olsson Ola , Brady Mark V. , Stjernman Martin , Smith Henrik G.
Published: September 2021

Most landscapes are comprised of multiple habitat types differing in the biodiversity they contain. This is certainly true for human modified landscapes, which are often a mix of habitats managed with different intensity, semi-natural habitats and even pristine habitats. To understand fundamental questions of how the composition of such landscapes affects biodiversity conservation, and to evaluate biodiversity consequences of policies that affect the composition of landscapes, there is a need for models able to translate information on biodiversity from individual habitats to landscape-wide predictions. However, this is complicated by species richness not being additive. We constructed a model to help analyze and solve this problem based on two simple assumptions. Firstly, that a habitat can be characterized by the biological community inhabiting it; i.e., which species occur and at what densities. Secondly, that the probability of a species occurring in a particular unit of land is dictated by its average density in the associated habitats, its spatial aggregation, and the size of the land unit. This model leads to a multidimensional species-area relation (one dimension per habitat). If the goal is to maximize species diversity at the landscape scale (γ-diversity), within a fixed area or under a limited budget, the model can be used to find the optimal allocation of the different habitats. In general, the optimal solution depends on the total size of the species pool of the different habitats, but also their similarity (β-diversity). If habitats are complementary (high β), a mix is usually preferred, even if one habitat is poorer (lower α diversity in one habitat). The model lends itself to economic analyses of biodiversity problems, without the need to monetarize biodiversity value, i.e., cost-effectiveness analysis. Land prices and management costs will affect the solution, such that the model can be used to estimate the number of species gained in relation to expenditure on each habitat. We illustrate the utility of the model by applying it to agricultural landscapes in southern Sweden and demonstrate how empirical monitoring data can be used to find the best habitat allocation for biodiversity conservation within and between landscapes.