bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthetic Microstructure Generation and Multiscale Analysis of Asphalt Concrete
Authors: Klimczak Marek , Cecot Witold
Published: January 2020

In this paper, we present an enhanced framework for the synthetic asphalt concrete (AC) microstructure generation for the numerical analysis purposes. It is based on the Voronoi tessellation concept with some necessary extensions that allow for the reliable generation of the aggregate particles of the given size distribution. The synthetic microstructure generation allows for faster numerical modeling of the novel materials. It can partially replace the X-ray computed tomography approach, which is frequently used in such analysis. Our framework is a kind of compilation of the known techniques with the enhancements applied to expedite the microstructure modeling process. Therefore, the generated microstructure is used in the numerical upscaling to model the macroscale asphalt concrete properties. We restrict ourselves (in this paper only) to the 2D elastic computations. We also assume the perfect bonding between these two materials and the static load for the sake of simplicity. The upscaling is performed by the multiscale finite element method (MsFEM). A short recapitulation of the MsFEM foundations as well as the numerical test comparing the overkill mesh solution with the upscaled one is provided in the paper. The test results confirm that the whole presented methodology can serve as a fast and reliable tool for the tests on novel asphalt mixtures and other heterogeneous materials. It can reduce the cost of the design process substituting some of the laboratory experiments, giving the opportunity to test the developed constitutive models and expedite the numerical analysis itself.
Sensitivity Analysis of Surface Energy Budget to Albedo Parameters in Seoul Metropolitan Area Using the Unified Model
Authors: Wie Jieun , Hong Seon-Ok , Byon Jae-Young , Ha Jong-Chul , Moon Byung-Kwon
Published: January 2020

The large population growth has significantly altered the thermal characteristics of the atmosphere, including decreased albedo and increased heat capacity; thus, urban areas experience unique climatic phenomena. We conducted sensitivity experiments using Unified Model Local Data Assimilation and Prediction-Met-Office-Reading Urban Surface Exchange Scheme (LDAPS-MORUSES) to investigate the response of surface energy budget to albedo changes in the Seoul Metropolitan Area. We compared 1.5-m temperature at 56 automatic weather station (AWS) sites and showed underestimations of approximately 0.5–2 K, but the diurnal cycle was well simulated. We changed the wall and road albedo parameters by ±50% from the default values for sensitivity experiments. With increasing albedo, 1.5-m temperature decreased by approximately 0.06 °C and 0.01 °C in urban and suburban areas, respectively. These changes are responses to decreased net radiation and sensible heat during daytime, whereas sensible heat mainly contributes to the surface cooling during nighttime. Furthermore, the decrease in albedo leads to altered vertical structure of potential temperature and atmospheric circulations at altitudes of 300–1000 m. Results show that albedo modification can affect not only surface temperature but also the entire urban boundary layer.
The Effects of Green Roofs on Outdoor Thermal Comfort, Urban Heat Island Mitigation and Energy Savings
Authors: Mutani Guglielmina , Todeschi Valeria
Published: January 2020

There is growing attention to the use of greenery in urban areas, in various forms and functions, as an instrument to reduce the impact of human activities on the urban environment. The aim of this study has been to investigate the use of green roofs as a strategy to reduce the urban heat island effect and to improve the thermal comfort of indoor and outdoor environments. The effects of the built-up environment, the presence of vegetation and green roofs, and the urban morphology of the city of Turin (Italy) have been assessed considering the land surface temperature distribution. This analysis has considered all the information recorded by the local weather stations and satellite images, and compares it with the geometrical and typological characteristics of the city in order to find correlations that confirm that greenery and vegetation improve the livability of an urban context. The results demonstrate that the land-surface temperature, and therefore the air temperature, tend to decrease as the green areas increase. This trend depends on the type of urban context. Based on the results of a green-roofs investigation of Turin, the existing and potential green roofs are respectively almost 300 (257,380 m2) and 15,450 (6,787,929 m2). Based on potential assessment, a strategy of priority was established according to the characteristics of building, to the presence of empty spaces, and to the identification of critical areas, in which the thermal comfort conditions are poor with low vegetation. This approach can be useful to help stakeholders, urban planners, and policy makers to effectively mitigate the urban heat island (UHI), improve the livability of the city, reduce greenhouse gas (GHG) emissions and gain thermal comfort conditions, and to identify policies and incentives to promote green roofs.
Accelerated Aging Characterization of Lithium-ion Cells: Using Sensitivity Analysis to Identify the Stress Factors Relevant to Cyclic Aging
Authors: Gewald Tanja , Candussio Adrian , Wildfeuer Leo , Lehmkuhl Dirk , Hahn Alexander , Lienkamp Markus
Published: January 2020

As storage technology in electric vehicles, lithium-ion cells are subject to a continuous aging process during their service life that, in the worst case, can lead to a premature system failure. Battery manufacturers thus have an interest in the aging prediction during the early design phase, for which semi-empirical aging models are often used. The progress of aging is dependent on the application-specific load profile, more precisely on the aging-relevant stress factors. Still, a literature review reveals a controversy on the aging-relevant stress factors to use as input parameters for the simulation models. It shows that, at present, a systematic and efficient procedure for stress factor selection is missing, as the aging characteristic is cell-specific. In this study, an accelerated sensitivity analysis as a prior step to aging modeling is proposed, which is transferable and allows to determine the actual aging-relevant stress factors for a specific lithium-ion cell. For the assessment of this accelerated approach, two test series with different acceleration levels and cell types are performed and evaluated. The results show that a certain amount of charge throughput, 100 equivalent full cycles in this case, is necessary to conduct a statistically significant sensitivity analysis.
Apoptotic Bodies: Particular Extracellular Vesicles Involved in Intercellular Communication
Authors: Battistelli Michela , Falcieri Elisabetta
Published: January 2020

In the last decade, a new method of cell–cell communication mediated by membranous extracellular vesicles (EVs) has emerged. EVs, including exosomes, microvesicles, and apoptotic bodies (ApoBDs), represent a new and important topic, because they are a means of communication between cells and they can also be involved in removing cellular contents. EVs are characterized by differences in size, origin, and content and different types have different functions. They appear as membranous sacs released by a variety of cells, in different physiological and patho-physiological conditions. Intringuingly, exosomes and microvesicles are a potent source of genetic information carriers between different cell types both within a species and even across a species barrier. New, and therefore still relatively poorly known vesicles are apoptotic bodies, on which numerous in-depth studies are needed in order to understand their role and possible function. In this review we would like to analyze their morpho-functional characteristics.
Synthesis of Chitosan-Polyvinyl Alcohol Biopolymers to Eliminate Fluorides from Water
Authors: Valdez-Alegría Cecilia , Fuentes-Rivas Rosa , García-Rivas José , Zavala Arce Rosa , Jiménez Núñez María , García-Gaitán Beatriz
Published: January 2020

The fluoride content in groundwater varies depending on geological configuration. Fluoride problems tend to occur in places where these minerals are most abundant in rocks. The objective of the present work was to synthesize four biopolymers based on chitosan-polyvinyl alcohol (Ch-PVA) cross-linked with sodium tripolyphosphate pentabasic (TPP) and ethylene glycol diglycidyl ether (EGDE) and determine their ability to remove fluoride from water. The characterization of the Ch-PVA beads was performed by way of Scanning Electron Microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). The percentage of humidity and the point of zero charge were determined. The Ch-PVA beads showed a surface area of 63.87 m2 g−1, a pore size of 7.6 nm, a point of zero charge of 7.4, and 98.6% humidity. The kinetic adsorption study was adjusted to the pseudo-second-order model and the adsorption equilibrium data were adjusted to the Freundlich adsorption isotherm, showing a maximum fluoride adsorption capacity of 12.64 mg g−1 at pH 7 and 30 °C, for the beads of Ch-PVA-NaOH-TPP. According to the thermodynamic parameters: −∆G°, +∆H° and −∆S°, fluoride adsorption is spontaneous, endothermic in nature and there is no random energy change in the solid/liquid interface during the adsorption process.
A Combined Proteomics, Metabolomics and In Vivo Analysis Approach for the Characterization of Probiotics in Large-Scale Production
Authors: Bianchi Laura , Laghi Luca , Correani Virginia , Schifano Emily , Landi Claudia , Uccelletti Daniela , Mattei Benedetta
Published: January 2020

The manufacturing processes of commercial probiotic strains may be affected in different ways in the attempt to optimize yield, costs, functionality, or stability, influencing gene expression, protein patterns, or metabolic output. Aim of this work is to compare different samples of a high concentration (450 billion bacteria) multispecies (8 strains) formulation produced at two different manufacturing sites, United States of America (US) and Italy (IT), by applying a combination of functional proteomics, metabolomics, and in vivo analyses. Several protein-profile differences were detected between IT- and US-made products, with Lactobacillus paracasei, Streptococcus thermophilus, and Bifidobacteria being the main affected probiotics/microorganisms. Performing proton nuclear magnetic spectroscopy (1H-NMR), some discrepancies in amino acid, lactate, betaine and sucrose concentrations were also reported between the two products. Finally, we investigated the health-promoting and antiaging effects of both products in the model organism Caenorhabditis elegans. The integration of omics platforms with in vivo analysis has emerged as a powerful tool to assess manufacturing procedures.
Anti-Migratory and Pro-Apoptotic Properties of Parvifloron D on Triple-Negative Breast Cancer Cells
Authors: Saraiva Nuno , Costa João G. , Reis Catarina , Almeida Nuno , Rijo Patrícia , Fernandes Ana Sofia
Published: January 2020

Medicinal plants are important sources of new bioactive compounds with potential anticancer activity. Parvifloron D (ParvD) is an abietane diterpenoid, isolated in high amounts from Plectranthus ecklonii Benth. Previous reports have suggested potential therapeutic properties for ParvD. ParvD has shown pro-apoptotic and cytotoxic effects in leukemia and melanoma cell lines. However, to the best of our knowledge, there are no studies in triple-negative breast cancer (TNBC) models. TNBC is a breast cancer subtype characterized by an aggressive behavior with poor clinical outcomes and weak overall therapeutic responses to the current treatment options. This work aimed at evaluating the anticancer effect of ParvD in MDA-MB-231 cells, a model of human TNBC. To obtain sufficient amounts of purified ParvD the efficiency of several extraction methods was compared. ParvD (0.1–10 µM) decreased cell viability in a concentration-dependent manner. Treatment with ParvD (5 µM) significantly increased the percentage of apoptotic nuclei and exposure to 3 µM ParvD increased the sub-G1 population. Since altered cell adherence, migration, and invasion are determinant processes for the formation of metastases, the effect of ParvD on these cellular processes was tested. Although treatment with ParvD (1 µM) had no effect on cell-substrate attachment, ParvD (1 µM) significantly reduced cell chemotaxis and invasion. This is the first report describing the proapoptotic effect of ParvD in TNBC cells. Moreover, for the first time we have shown that ParvD reduces cell motility, unraveling potential anti-metastatic properties.
Back to GroEL-Assisted Protein Folding: GroES Binding-Induced Displacement of Denatured Proteins from GroEL to Bulk Solution
Authors: Marchenkov Victor , Gorokhovatsky Andrey , Marchenko Natalia , Ivashina Tanya , Semisotnov Gennady
Published: January 2020

The main events in chaperone-assisted protein folding are the binding and ligand-induced release of substrate proteins. Here, we studied the location of denatured proteins previously bound to the GroEL chaperonin resulting from the action of the GroES co-chaperonin in the presence of Mg-ATP. Fluorescein-labeled denatured proteins (α-lactalbumin, lysozyme, serum albumin, and pepsin in the presence of thiol reagents at neutral pH, as well as an early refolding intermediate of malate dehydrogenase) were used to reveal the effect of GroES on their interaction with GroEL. Native electrophoresis has demonstrated that these proteins tend to be released from the GroEL-GroES complex. With the use of biotin- and fluorescein-labeled denatured proteins and streptavidin fused with luciferase aequorin (the so-called streptavidin trap), the presence of denatured proteins in bulk solution after GroES and Mg-ATP addition has been confirmed. The time of GroES-induced dissociation of a denatured protein from the GroEL surface was estimated using the stopped-flow technique and found to be much shorter than the proposed time of the GroEL ATPase cycle.
A SWOT Analysis of the Use of BIM Technology in the Polish Construction Industry
Authors: Zima Krzysztof , Plebankiewicz Edyta , Wieczorek Damian
Published: January 2020

The present paper presents a SWOT analysis, the aim of which is to evaluate the strategic implementation of BIM technology in the construction industry in Poland. The authors created a SWOT matrix presenting strengths, weaknesses, opportunities, and risks associated with the use of BIM. Using literature analyses, own experience, and market reports, all elements of the SWOT matrix are described in detail. Basic indicators characterizing the strategic position of BIM on the Polish construction market are calculated. Finally, the matrix of strategic tasks and actions that should be applied in order to promote and develop BIM in Poland are defined.
1H-NMR Based Serum Metabolomics Highlights Different Specific Biomarkers between Early and Advanced Hepatocellular Carcinoma Stages
Authors: Casadei-Gardini Andrea , Del Coco Laura , Marisi Giorgia , Conti Fabio , Rovesti Giulia , Ulivi Paola , Canale Matteo , Frassineti Giovanni Luca , Foschi Francesco Giuseppe , Longo Serena , Fanizzi Francesco Paolo , Giudetti Anna Maria
Published: January 2020

The application of non-targeted serum metabolomics profiling represents a noninvasive tool to identify new clinical biomarkers and to provide early diagnostic differentiation, and insight into the pathological mechanisms underlying hepatocellular carcinoma (HCC) progression. In this study, we used proton Nuclear Magnetic Resonance (1H-NMR) Spectroscopy and multivariate data analysis to profile the serum metabolome of 64 HCC patients, in early (n = 28) and advanced (n = 36) disease stages. We found that 1H-NMR metabolomics profiling could discriminate early from advanced HCC patients with a cross-validated accuracy close to 100%. Orthogonal partial least squares discriminant analysis (OPLS-DA) showed significant changes in serum glucose, lactate, lipids and some amino acids, such as alanine, glutamine, 1-methylhistidine, lysine and valine levels between advanced and early HCC patients. Moreover, in early HCC patients, Kaplan–Meier analysis highlighted the serum tyrosine level as a predictor for overall survival (OS). Overall, our analysis identified a set of metabolites with possible clinical and biological implication in HCC pathophysiology.
Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma
Authors: Akter Mahmuda , Jangra Anshika , Choi Seung Ah , Choi Eun Ha , Han Ihn
Published: January 2020

Nonthermal plasma is a promising novel therapy for the alteration of biological and clinical functions of cells and tissues, including apoptosis and inhibition of tumor progression. This therapy generates reactive oxygen and nitrogen species (RONS), which play a major role in anticancer effects. Previous research has verified that plasma jets can selectively induce apoptosis in various cancer cells, suggesting that it could be a potentially effective novel therapy in combination with or as an alternative to conventional therapeutic methods. In this study, we determined the effects of nonthermal air soft plasma jets on a U87 MG brain cancer cell line, including the dose- and time-dependent effects and the physicochemical and biological correlation between the RONS cascade and p38/mitogen-activated protein kinase (MAPK) signaling pathway, which contribute to apoptosis. The results indicated that soft plasma jets efficiently inhibit cell proliferation and induce apoptosis in U87 MG cells but have minimal effects on astrocytes. These findings revealed that soft plasma jets produce a potent cytotoxic effect via the initiation of cell cycle arrest and apoptosis. The production of reactive oxygen species (ROS) in cells was tested, and an intracellular ROS scavenger, N-acetyl cysteine (NAC), was examined. Our results suggested that soft plasma jets could potentially be used as an effective approach for anticancer therapy.
Sterol O-Acyl Transferase 1 as a Prognostic Marker of Adrenocortical Carcinoma
Authors: Lacombe Amanda Meneses Ferreira , Soares Iberê Cauduro , Mariani Beatriz Marinho de Paula , Nishi Mirian Yumie , Bezerra-Neto João Evangelista , Charchar Helaine da Silva , Brondani Vania Balderrama , Tanno Fabio , Srougi Victor , Chambo José Luiz , Costa de Freitas Ricardo Miguel , Mendonca Berenice Bilharinho , Hoff Ana O. , Almeida Madson Q. , Weigand Isabel , Kroiss Matthias , Zerbini Maria Claudia Nogueira , Fragoso Maria Candida Barisson Villares
Published: January 2020

Adrenocortical carcinoma (ACC) is a rare endocrine malignancy with an unfavorable prognosis. Despite the poor prognosis in the majority of patients, no improvements in treatment strategies have been achieved. Therefore, the discovery of new prognostic biomarkers is of enormous interest. Sterol-O-acyl transferase 1 (SOAT1) is involved in cholesterol esterification and lipid droplet formation. Recently, it was demonstrated that SOAT1 inhibition leads to impaired steroidogenesis and cell viability in ACC. To date, no studies have addressed the impact of SOAT1 expression on ACC prognosis and clinical outcomes. We evaluated SOAT1 expression by quantitative real-time polymerase chain reaction and immunohistochemistry in a tissue microarray of 112 ACCs (Weiss score ≥ 3) from adults treated in a single tertiary center in Brazil. Two independent pathologists evaluated the immunohistochemistry results through a semiquantitative approach (0–4). We aimed to evaluate the correlation between SOAT1 expression and clinical, biochemical and anatomopathological parameters, recurrence-free survival (RFS), progression-free survival (PFS), and overall survival (OS). SOAT1 protein expression was heterogeneous in this cohort, 37.5% of the ACCs demonstrated a strong SOAT1 protein expression (score > 2), while 62.5% demonstrated a weak or absent protein expression (score ≤ 2). Strong SOAT1 protein expression correlated with features of high aggressiveness in ACC, such as excessive tumor cortisol secretion (p = 0.01), an advanced disease stage [European Network for the Study of Adrenal Tumors (ENSAT) staging system 3 and 4 (p = 0.011)] and a high Ki67 index (p = 0.002). In multivariate analysis, strong SOAT1 protein expression was an independent predictor of a reduced OS (hazard ratio (HR) 2.15, confidence interval (CI) 95% 1.26–3.66; p = 0.005) in all patients (n = 112), and a reduced RFS (HR 2.1, CI 95% 1.09–4.06; p = 0.027) in patients with localized disease at diagnosis (n = 83). Our findings demonstrated that SOAT1 protein expression has prognostic value in ACC and reinforced the importance of investigating SOAT1 as a possible therapeutic target for patients with ACC.
Erratum: Neuromedin U: A Small Peptide in the Big World of Cancer. Cancers 2019, 11, 1312
Authors: Przygodzka Patrycja , Soboska Kamila , Sochacka Ewelina , Boncela Joanna
Published: January 2020

The authors wish to make the following corrections to this paper [...]
Survival-Associated Metabolic Genes in Human Papillomavirus-Positive Head and Neck Cancers
Authors: Prusinkiewicz Martin A. , Gameiro Steven F. , Ghasemi Farhad , Dodge Mackenzie J. , Zeng Peter Y. F. , Maekebay Hanna , Barrett John W. , Nichols Anthony C. , Mymryk Joe S.
Published: January 2020

Human papillomavirus (HPV) causes an increasing number of head and neck squamous cell carcinomas (HNSCCs). Altered metabolism contributes to patient prognosis, but the impact of HPV status on HNSCC metabolism remains relatively uncharacterized. We hypothesize that metabolism-related gene expression differences unique to HPV-positive HNSCC influences patient survival. The Cancer Genome Atlas RNA-seq data from primary HNSCC patient samples were categorized as 73 HPV-positive, 442 HPV-negative, and 43 normal-adjacent control tissues. We analyzed 229 metabolic genes and identified numerous differentially expressed genes between HPV-positive and negative HNSCC patients. HPV-positive carcinomas exhibited lower expression levels of genes involved in glycolysis and higher levels of genes involved in the tricarboxylic acid cycle, oxidative phosphorylation, and β-oxidation than the HPV-negative carcinomas. Importantly, reduced expression of the metabolism-related genes SDHC, COX7A1, COX16, COX17, ELOVL6, GOT2, and SLC16A2 were correlated with improved patient survival only in the HPV-positive group. This work suggests that specific transcriptional alterations in metabolic genes may serve as predictive biomarkers of patient outcome and identifies potential targets for novel therapeutic intervention in HPV-positive head and neck cancers.
DKK1 Induced by 1,25D3 Is Required for the Mineralization of Osteoblasts
Authors: Jo Sungsin , Yoon Subin , Lee So Young , Kim So Yeon , Park Hyosun , Han Jinil , Choi Sung Hoon , Han Joong-Soo , Yang Jae-Hyuk , Kim Tae-Hwan
Published: January 2020

1α,25-dihydroxyvitamin D3 (1,25D3), the most popular drug for osteoporosis treatment, drives osteoblast differentiation and bone mineralization. Wnt/β-catenin signaling is involved in commitment and differentiation of osteoblasts, but the role of the Dickkopf-related protein 1 (DKK1), a Wnt antagonist, in osteoblasts remains unknown. Here, we demonstrate the molecular mechanism of DKK1 induction by 1,25D3 and its physiological role during osteoblast differentiation. 1,25D3 markedly promoted the expression of both CCAAT/enhancer binding protein beta (C/EBPβ) and DKK1 at day 7 during osteoblast differentiation. Interestingly, mRNA and protein levels of C/EBPβ and DKK1 in osteoblasts were elevated by 1,25D3. We also found that C/EBPβ, in response to 1,25D3, directly binds to the human DKK1 promoter. Knockdown of C/EBPβ downregulated the expression of DKK1 in osteoblasts, which was partially reversed by 1,25D3. In contrast, overexpression of C/EBPβ upregulated DKK1 expression in osteoblasts, which was enhanced by 1,25D3. Furthermore, 1,25D3 treatment in osteoblasts stimulated secretion of DKK1 protein within the endoplasmic reticulum to extracellular. Intriguingly, blocking DKK1 attenuated calcified nodule formation in mineralized osteoblasts, but not ALP activity or collagen synthesis. Taken together, these observations suggest that 1,25D3 promotes the mineralization of osteoblasts through activation of DKK1 followed by an increase of C/EBPβ.
Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes
Authors: Gelaude Armance , Slaby Sylvain , Cailliau Katia , Marin Matthieu , Lescuyer-Rousseau Arlette , Molinaro Caroline , Nevoral Jan , Kučerová-Chrpová Veronica , Sedmikova Marketa , Petr Jaroslav , Martoriati Alain , Bodart Jean-François
Published: January 2020

The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.
Applications of Genome Editing Technology in Research on Chromosome Aneuploidy Disorders
Authors: Akutsu Silvia Natsuko , Fujita Kazumasa , Tomioka Keita , Miyamoto Tatsuo , Matsuura Shinya
Published: January 2020

Chromosomal segregation errors in germ cells and early embryonic development underlie aneuploidies, which are numerical chromosomal abnormalities causing fetal absorption, developmental anomalies, and carcinogenesis. It has been considered that human aneuploidy disorders cannot be resolved by radical treatment. However, recent studies have demonstrated that aneuploidies can be rescued to a normal diploid state using genetic engineering in cultured cells. Here, we summarize a series of studies mainly applying genome editing to eliminate an extra copy of human chromosome 21, the cause of the most common constitutional aneuploidy disorder Down syndrome. We also present findings on induced pluripotent stem cell reprogramming, which has been shown to be one of the most promising technologies for converting aneuploidies into normal diploidy without the risk of genetic alterations such as genome editing-mediated off-target effects.
Cellular and Molecular Differences between HFpEF and HFrEF: A Step Ahead in an Improved Pathological Understanding
Authors: Simmonds Steven J. , Cuijpers Ilona , Heymans Stephane , Jones Elizabeth A. V.
Published: January 2020

Heart failure (HF) is the most rapidly growing cardiovascular health burden worldwide. HF can be classified into three groups based on the percentage of the ejection fraction (EF): heart failure with reduced EF (HFrEF), heart failure with mid-range—also called mildly reduced EF— (HFmrEF), and heart failure with preserved ejection fraction (HFpEF). HFmrEF can progress into either HFrEF or HFpEF, but its phenotype is dominated by coronary artery disease, as in HFrEF. HFrEF and HFpEF present with differences in both the development and progression of the disease secondary to changes at the cellular and molecular level. While recent medical advances have resulted in efficient and specific treatments for HFrEF, these treatments lack efficacy for HFpEF management. These differential response rates, coupled to increasing rates of HF, highlight the significant need to understand the unique pathogenesis of HFrEF and HFpEF. In this review, we summarize the differences in pathological development of HFrEF and HFpEF, focussing on disease-specific aspects of inflammation and endothelial function, cardiomyocyte hypertrophy and death, alterations in the giant spring titin, and fibrosis. We highlight the areas of difference between the two diseases with the aim of guiding research efforts for novel therapeutics in HFrEF and HFpEF.
Mechanical Forces as Determinants of Disseminated Metastatic Cell Fate
Authors: Montagner Marco , Dupont Sirio
Published: January 2020

Disseminated metastatic cancer cells represent one of the most relevant causes of disease relapse and associated death for cancer patients, and a therapeutic target of the highest priority. Still, our understanding of how disseminated cancer cells survive in the foreign metastatic environment, and eventually cause metastatic outgrowth, remains rather limited. In this review we focus on the cell microenvironment as a key regulator of cell behavior at the metastatic site, and especially on the mechanical properties of the extracellular matrix and associated integrin signaling. We discuss available evidence pointing to a pervasive role of extracellular matrix (ECM) mechanical properties in regulating cancer cell proliferation and survival after dissemination, and propose that this might represent an important bottleneck for cells invading and establishing into a novel tissue. We point to the known molecular players, how these might contribute to modulate the mechanical properties of the metastatic environment, and the response of cells to these cues. Finally, we propose that emerging knowledge on the physical interaction of disseminated metastatic cells and on the downstream mechanotransduction pathways, including YAP/TAZ (Yes-associated protein-1 and WW-domain transcription activator 1) and MRTFs (Myocardin-related transcription factors), may help to identify novel approaches for therapy.
PPARγ-Independent Side Effects of Thiazolidinediones on Mitochondrial Redox State in Rat Isolated Hearts
Authors: Riess Matthias L. , Elorbany Reem , Weihrauch Dorothee , Stowe David F. , Camara Amadou K.S.
Published: January 2020

The effect of anti-diabetic thiazolidinediones (TZDs) on contributing to heart failure and cardiac ischemia/reperfusion (IR) injury is controversial. In this study we investigated the effect of select TZDs on myocardial and mitochondrial function in Brown Norway rat isolated hearts. In a first set of experiments, the TZD rosiglitazone was given acutely before global myocardial IR, and pre- and post-IR function and infarct size were assessed. In a second set of experiments, different concentrations of rosiglitazone and pioglitazone were administered in the presence or absence of the specific PPARγ antagonist GW9662, and their effects on the mitochondrial redox state were measured by online NADH and FAD autofluorescence. The administration of rosiglitazone did not significantly affect myocardial function except for transiently increasing coronary flow, but it increased IR injury compared to the control hearts. Both TZDs resulted in dose-dependent, reversible increases in mitochondrial oxidation which was not attenuated by GW9662. Taken together, these data suggest that TZDs cause excessive mitochondrial uncoupling by a PPARγ-independent mechanism. Acute rosiglitazone administration before IR was associated with enhanced cardiac injury. If translated clinically, susceptible patients on PPARγ agonists may experience enhanced myocardial IR injury by mitochondrial dysfunction.
Meyer-Rod Coated 2D Single-Crystalline Copper Nanoplate Film with Intensive Pulsed Light for Flexible Electrode
Authors: Go Myeongjong , Alam Asrar , Choie Ho Kwang , Zhong Zhaoyang , Lee Keun Hyung , Seo Youngjae , Hwang Byungil , Woo Kyoohee , Kim Tae-Wook , Lim Sooman
Published: January 2020

Copper is widely used because it is inexpensive, abundant, and highly conductive. However, most copper used in industrial coating processes is in the form of circular powder, which is problematic for large area, high conductive coatings. In this work, 2D single-crystalline Cu nanoplates (Cu NPLs) were synthesized and a systematic study on coating with large-scale Cu NPLs using a Meyer-rod coating process was performed. The rheological behaviors of the Cu solution with various concentrations, surface tensions, and speeds were analyzed using Ca and Re numbers to optimize coating conditions. In addition, the effect of intensive pulse light (IPL) to sinter the coper film within a 1 s timeframe was also investigated in order to be able to produce an electrode in the shortest possible time which is applicable to industry. Finally, the Meyer-rod coated electrode was utilized in an electrochemical luminescence (ECL) device.
A Novel Security Protocol for Wireless Sensor Networks with Cooperative Communication
Authors: Al Hayajneh Abdullah , Bhuiyan Md Zakirul Alam , McAndrew Ian
Published: January 2020

This paper builds upon the foundation and clarifies specifications for a necessary security protocol in Wireless Sensor Networks (WSNs) with cooperative communications. It is designed to enhance performance and resiliency against cyber-attacks. Recent literature has shown that developing a WSN with Cooperative Communication greatly increases the performance of the network, but also exposes new vulnerabilities. The technique operates by transmitting packets of data to neighboring relay nodes in a cooperative fashion to reach the destination. In this paper, we consider security issues in WSNs with cooperative communication on each layer of the OSI model: physical layer, data link layer, network layer, service (topology) layer, and application layer. For each layer, we clarify the main task, enumerate the main attacks and threats, specify the primary security approaches and techniques (if any), and discuss possible new attacks and problems that may arise with the use of cooperative communications. Furthermore, we show for some attacks (e.g., jamming, packet dropping, and wormhole) that using cooperative communication improves the network resiliency and reliability. Finally, we propose a security protocol that addresses many of these shortcomings, while outlining the remaining issues that need further work and research.
Crystal Structural Analysis of DL-Mandelate Salt of Carvedilol and Its Correlation with Physicochemical Properties
Authors: Hata Nanami , Furuishi Takayuki , Tamboli Majid I. , Ishizaki Momiji , Umeda Daiki , Fukuzawa Kaori , Yonemochi Etsuo
Published: January 2020

A 1:1 salt of carvedilol (CVD), an anti-hypertensive drug, with DL-mandelic acid (DL-MA) was crystallized from ethanol and the structure was characterized by X-ray single-crystal diffraction, revealing salt formation by transfer of an acidic proton from the COOH group of MA to the aliphatic (acyclic) secondary amino NH group of CVD. The crystal structure is triclinic, with a P-1 space group and unit cell parameters a = 9.8416(5) Å, b = 11.4689(5) Å, c = 14.0746(7) Å, α = 108.595(8), β = 95.182(7), γ = 107.323(8), V = 1406.95(15) Å3, and Z = 2. The asymmetric unit contained one protonated CVD and one MA anion, linked via an N+–H∙∙∙O¯ strong hydrogen bond and a ratio of 1:1. As previously reported, the thermal, spectroscopic, and powder X-ray diffraction properties of the salt of CVD with DL-MA (CVD_DL-MA) differed from CVD alone. The intrinsic dissolution rate of CVD_DL-MA was about 10.7 times faster than CVD alone in a pH 6.8 buffer.
Competitive Interactions of Flowering Rush (Butomus umbellatus L.) Cytotypes in Submersed and Emergent Experimental Aquatic Plant Communities
Authors: Harms Nathan E.
Published: January 2020

The ability to invade communities in a variety of habitats (e.g., along a depth gradient) may facilitate establishment and spread of invasive plants, but how multiple lineages of a species perform under varying conditions is understudied. A series of greenhouse common garden experiments were conducted in which six diploid and four triploid populations of the aquatic invasive plant Butomus umbellatus L. (Butomaceae) were grown in submersed or emergent conditions, in monoculture or in a multispecies community, to compare establishment and productivity of cytotypes under competition. Diploid biomass overall was 12 times higher than triploids in the submersed experiment and three times higher in the emergent experiment. Diploid shoot:root ratio was double that of triploid plants in submersed conditions overall, and double in emergent conditions in monoculture. Relative interaction intensities (RII) indicated that triploid plants were sixteen times more negatively impacted by competition under submersed conditions but diploid plants were twice as impacted under emergent conditions. Recipient communities were similarly negatively impacted by B. umbellatus cytotypes. This study supports the idea that diploid and triploid B. umbellatus plants are equally capable of invading emergent communities, but that diploid plants may be better adapted for invading in submersed habitats. However, consistently lower shoot:root ratios in both monoculture and in communities suggests that triploid plants may be better-adapted competitors in the long term due to increased resource allocation to roots. This represents the first examination into the role of cytotype and habitat on competitive interactions of B. umbellatus.
What’s New on Quantitative CT Analysis as a Tool to Predict Growth in Persistent Pulmonary Subsolid Nodules? A Literature Review
Authors: Borghesi Andrea , Michelini Silvia , Golemi Salvatore , Scrimieri Alessandra , Maroldi Roberto
Published: January 2020

Pulmonary subsolid nodules (SSNs) are observed not infrequently on thin-section chest computed tomography (CT) images. SSNs persisting after a follow-up period of three to six months have a high likelihood of being pre-malignant or malignant lesions. Malignant SSNs usually represent the histologic spectrum of pulmonary adenocarcinomas, and pulmonary adenocarcinomas presenting as SSNs exhibit quite heterogeneous behavior. In fact, while most lesions show an indolent course and may grow very slowly or remain stable for many years, others may exhibit significant growth in a relatively short time. Therefore, it is not yet clear which persistent SSNs should be surgically removed and for how many years stable SSNs should be monitored. In order to solve these two open issues, the use of quantitative analysis has been proposed to define the “tailored” management of persistent SSNs. The main purpose of this review was to summarize recent results about quantitative CT analysis as a diagnostic tool for predicting the behavior of persistent SSNs. Thus, a literature search was conducted in PubMed/MEDLINE, Scopus, and Web of Science databases to find original articles published from January 2014 to October 2019. The results of the selected studies are presented and compared in a narrative way.
Disease-Specific Survival of Type I and Type II Epithelial Ovarian Cancers—Stage Challenges Categorical Assignments of Indolence & Aggressiveness
Authors: Pavlik Edward J. , Smith Christopher , Dennis Taylor S. , Harvey Elizabeth , Huang Bin , Chen Quan , West Piecoro Dava , Burgess Brian T. , McDowell Anthony , Gorski Justin , Baldwin Lauren A. , Miller Rachel W. , DeSimone Christopher P. , Dietrich Charles , Gallion Holly H. , Ueland Frederick R. , van Nagell John R.
Published: January 2020

Epithelial ovarian cancers (EOC) consist of several sub-types based on histology, clinical, molecular and epidemiological features that are termed “histo-types”, which can be categorized into less aggressive Type I and more aggressive Type II malignancies. This investigation evaluated the disease-specific survival (DSS) of women with Type I and II EOC using histo-type, grade, and stage. A total of 47,789 EOC cases were identified in the National Cancer Institute’s Surveillance, Epidemiology, and End Results (SEER) data. Survival analysis and log rank test were performed to identify a 2-tiered classification (grade 1 vs. grade 2 & 3) for serous EOC. DSS of early stage serous EOC for grade 2 was significantly different from grade 3 indicating that a 2-tier classification for serous EOC applied only to late stage. DSS of Type I EOC was much better than Type II. However, DSS was 33–52% lower with late stage Type I than with early stage Type I indicating that Type I ovarian cancers should not be considered indolent. Early stage Type II EOC had much better DSS than late stage Type II stressing that stage has a large role in survival of both Type I and II EOC.
A Standardized Project Gutenberg Corpus for Statistical Analysis of Natural Language and Quantitative Linguistics
Authors: Gerlach Martin , Font-Clos Francesc
Published: January 2020

The use of Project Gutenberg (PG) as a text corpus has been extremely popular in statistical analysis of language for more than 25 years. However, in contrast to other major linguistic datasets of similar importance, no consensual full version of PG exists to date. In fact, most PG studies so far either consider only a small number of manually selected books, leading to potential biased subsets, or employ vastly different pre-processing strategies (often specified in insufficient details), raising concerns regarding the reproducibility of published results. In order to address these shortcomings, here we present the Standardized Project Gutenberg Corpus (SPGC), an open science approach to a curated version of the complete PG data containing more than 50,000 books and more than 3 × 10 9 word-tokens. Using different sources of annotated metadata, we not only provide a broad characterization of the content of PG, but also show different examples highlighting the potential of SPGC for investigating language variability across time, subjects, and authors. We publish our methodology in detail, the code to download and process the data, as well as the obtained corpus itself on three different levels of granularity (raw text, timeseries of word tokens, and counts of words). In this way, we provide a reproducible, pre-processed, full-size version of Project Gutenberg as a new scientific resource for corpus linguistics, natural language processing, and information retrieval.
Macroprudential Policy in a Heterogeneous Environment—An Application of Agent-Based Approach in Systemic Risk Modelling
Authors: Kaszowska-Mojsa Jagoda , Pipień Mateusz
Published: January 2020

Assessment of welfare effects of macroprudential policy seems the most important application of the Dynamic Stochastic General Equilibrium (DSGE) framework of macro-modelling. In particular, the DSGE-3D model, with three layers of default (3D), was developed and used by the European Systemic Risk Board and European Central Bank as a reference tool to formally model the financial cycle as well as to analyze effects of macroprudential policies. Despite the extreme importance of incorporating financial constraints in Real Business Cycle (RBC) models, the resulting DSGE-3D construct still embraces the representative agent idea, making serious analyses of diversity of economic entities impossible. In this paper, we present an alternative to DSGE modelling that seriously departs from the assumption of the representativeness of agents. Within an Agent Based Modelling (ABM) framework, we build an environment suitable for performing counterfactual simulations of the impact of macroprudential policy on the economy, financial system and society. We contribute to the existing literature by presenting an ABM model with broad insight into heterogeneity of agents. We show the stabilizing effects of macroprudential policies in the case of economic or financial distress.
Area-Efficient Error Detection Structure for Linear Feedback Shift Registers
Authors: Shin Hwasoo , Choi Soyeon , Park Jiwoon , Kong Byeong Yong , Yoo Hoyoung
Published: January 2020

This paper presents a novel error detection linear feedback shift register (ED-LFSR), which can be used to realize error detection with a small hardware overhead for various applications such as error-correction codes, encryption algorithms and pseudo-random number generation. Although the traditional redundancy methods allow the incorporation of the error detection/correction capability in the original LFSRs, they suffer from a considerable amount of hardware overheads. The proposed ED-LFSR alleviates such problems by employing the parity check technique. The experimental results indicate that the proposed ED-LFSR requires an additional area of only 31.1% compared to that required by the conventional LFSR and it saves 39.1% and 31.9% of the resources compared to the corresponding utilization of the hardware and time redundancy methods.
Spatiotemporal Feature Learning Based Hour-Ahead Load Forecasting for Energy Internet
Authors: Du Liufeng , Zhang Linghua , Wang Xu
Published: January 2020

In this paper, we analyze the characteristics of the load forecasting task in the Energy Internet context and the deficiencies of existing methods and then propose a data driven approach for one-hour-ahead load forecasting based on the deep learning paradigm. The proposed scheme involves three aspects. First, we formulate a historical load matrix (HLM) with spatiotemporal correlation combined with the EI scenario and then create a three-dimensional historical load tensor (HLT) that contains the HLMs for multiple consecutive time points before the forecasted hour. Second, we preprocess the HLT leveraging a novel low rank decomposition algorithm and different load gradients, aiming to provide a forecasting model with richer input data. Third, we develop a deep forecasting framework (called the 3D CNN-GRU) featuring a feature learning module followed by a regression module, in which the 3D convolutional neural network (3D CNN) is used to extract the desired feature sequences with time attributes, while the gated recurrent unit (GRU) is responsible for mapping the sequences to the forecast values. By feeding the corresponding load label into the 3D CNN-GRU, our proposed scheme can carry out forecasting tasks for any zone covered by the HLM. The results of self-evaluation and a comparison with several state-of-the-art methods demonstrate the superiority of the proposed scheme.
Real-Time Image Stabilization Method Based on Optical Flow and Binary Point Feature Matching
Authors: Deng Zilong , Yang Dongxiao , Zhang Xiaohu , Dong Yuguang , Liu Chengbo , Shen Qiang
Published: January 2020

The strap-down missile-borne image guidance system can be easily affected by the unwanted jitters of the motion of the camera, and the subsequent recognition and tracking functions are also influenced, thus severely affecting the navigation accuracy of the image guidance system. So, a real-time image stabilization technology is needed to help improve the image quality of the image guidance system. To satisfy the real-time and accuracy requirements of image stabilization in the strap-down missile-borne image guidance system, an image stabilization method based on optical flow and image matching with binary feature descriptors is proposed. The global motion of consecutive frames is estimated by the pyramid Lucas-Kanade (LK) optical flow algorithm, and the interval frames image matching based on fast retina keypoint (FREAK) algorithm is used to reduce the cumulative trajectory error. A Kalman filter is designed to smooth the trajectory, which is conducive to fitting to the main motion of the guidance system. Simulations have been carried out, and the results show that the proposed algorithm improves the accuracy and real-time performance simultaneously compared to the state-of-art algorithms.