bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Sistem Pengendalian Internal Terhadap Mekanisme Pencairan Dana Pihak Ketiga Pada Dinas Bina Marga dan Bina Konstruksi Provinsi Lampung
Authors: Pane Dilla Amadea , Luthfi Muhammad , Alansori Apip
Published: March 2024

Penelitian ini bertujuan untuk mengetahui pengaruh Sistem Pengendalian Internal terhadap Mekanisme Pencairan Dana Pihak Ketiga. Penelitian ini menggunakan data hasil dari observasi, wawancara dan kuesioner yang dibagikan ke Dinas Bina Marga dan Bina Konstruksi Provinsi Lampung.Metode pengumpulan data yang digunakan dalam penelitian ini menggunakan metode survei kuesioner. Survei kuesioner merupakan metode survei dengan menggunakan kuesioner penelitian. Kuesioner adalah satu set pertanyaan yang tersusun secara sistematis dan standar sehingga pertanyaan yang sama dapat diajukan kepada setiap responden.Hasil penelitian menunjukkan bahwa Variabel Lingkungan Pengendalian (X1) berpengaruh signifikan terhadap Mekanisme Pencairan Dana Pihak Ketiga, Variabel Penilaian Resiko (X2) berpengaruh signifikan terhadap Mekanisme Pencairan Dana Pihak Ketiga, Variabel Prosedur Pengendalian (X3) berpengaruh signifikan terhadap Mekanisme Pencairan Dana Pihak Ketiga, Variabel Pemantauan atau Monitoring (X4) berpengaruh signifikan terhadap Mekanisme Pencairan Dana Pihak Ketiga, Variabel Informasi dan Komunikasi (X5) berpengaruh signifikan terhadap Mekanisme Pencairan Dana Pihak Ketiga.
Analisis Penerapan Metode Activity Based Costing Dalam Penentuan Harga Pokok Produksi (Studi Kasus Pada Oemar Bakery Lampung)
Authors: Fahrizal Azli , Ichdan Dany Amrul , Indrawati Ani
Published: March 2024

Sistem Activity Based Costing (ABC) dapat menyediakan informasi perhitungan biaya yang lebih baik dan dapat membantu manajemen mengelola perusahaan secara efisien serta memperoleh pemahaman yang lebih baik atas kekuatan, kelemahan dan keunggulan kompetitif perusahaan. Sehingga dengan metode ABC dapat menyajikan informasi harga pokok produksi secara cermat dan akurat bagi kepentingan manajemen. Tujuan penelitian ini mengetahui perbandingan HPP dengan menggunakan metode yang diterapkan Raihan Bakery And Cake Shop Medan saat ini dengan harga pokok yang dihasilkan dengan metode ABC. Metode analisis yang digunakan adalah analisa kualitatif. Hasil penelitian menunjukan bahwa perhitungan harga pokok produksi (HPP) menggunakan ABC memberikan hasil yang lebih kecil dari cara tradisional pada Raihan Bakery And Cake Shop Medan. Sistem tradisional memberikan perhitungan harga pokok produksi yang lebih tinggi dibandingkan dengan metode Activity Based Costing, dikarenakan perhitungan dengan sistem tradisional hanya menggunakan satu cost driver sehingga banyak terjadi distorsi biaya dan menghasilkan perhitungan harga pokok produksi yang tidak relevan. Perusahaan sebaiknya mengevaluasi kembali sistem pembebanan biaya dalam menentukan harga pokok produksi (HPP).
Marcus & Burner, eds. America Firsthand
Authors: Rulon Philip Reed
Published: March 2024

The editors of America Firsthand believe that the "past is the present writ small." In this two volume set designed as supplementary reading for the survey course, Robert Marcus and David Burner have dug deeply into the historical past to find diary entries, letters, speeches, government documents, and specialized excerpts from vintage literature and monographs to illustrate that life today bears some marked similarities to the generations of yesteryear.
Pengaruh Ujian SOOCA terhadap Tingkat Kecemasan Mahasiswa Fakultas Kedokteran Universitas Muslim Indonesia
Authors: Namirah Namirah , Erwin Rachman Mochammad , Wahyu Sri , P Amir Suliati , Nurul Aisyah Windy
Published: March 2024

Kecemasan adalah perasaan tegang dan khawatir akibat reaksi terhadap ketidakmampuan untuk memecahkan masalah atau perasaan tidak aman. Menurut penelitian Mancevska, mahasiswa kedokteran tahun pertama mengalami tingkat kecemasan yang tinggi. Kecemasan pada tahun pertama dapat disebabkan oleh banyak hal, dan mahasiswa di lingkungan perkuliahan harus beradaptasi dengan lingkungan yang berbeda dari lingkungan sebelumnya yaitu lingkungan SMA. Tujuan penelitian untuk mengetahui adanya pengaruh ujian SOOCA (student objective oral case analysis) terhadap tingkat kecemasan mahasiswa preklinik angkatan 2020 Fakultas Kedokteran Universitas Muslim Indonesia. Metode yang di gunakan penelitian kuantitatif analitik observasional dengan menggunakan desain studi cross sectional. Berdasarkan Dari 157 mahasiswa yang mengisi kuisioner sebanyak 39 mahasiswa (24,8%) tidak mengalami kecemasan, sebanyak 118 mahasiswa (75,2%) mengalami kecemasan ringan, kecemasan sedang, kecemasan berat, dan kecemasan sangat berat. Berdasarkan analisis didapatkan p value (0,285) > 0,05 menunjukkan tidak adanya pengaruh Ujian SOOCA (student Objective Oral Case Analysis) terhadap tingkat kecemasan mahasiswa Fakultas Kedokteran Universitas Muslim Indonesia Angkatan 2020. Dari hasil tersebut tidak terdapat pengaruh ujian SOOCA (student objective oral case analysis) terhadap tingkat kecemasan mahasiswa Fakultas Kedokteran Universitas Muslim Indonesia angkatan 2020.
Karakteristik Penderita Kanker Kolorektal Di Rumah Sakit Pendidikan Ibnu Sina Makassar Tahun 2022
Authors: Asmaul Husnah  , Andi Kartini Eka Yanti  , Arina Fathiyyah Arifin  , Berry Erida Hasbi  , Dzul Ikram
Published: March 2024

Di Indonesia, kanker kolorektal merupakan pembunuh utama. Mengacu pada tumor ganas di usus besar dan rektum, kami mengatakan bahwa kanker ini berkembang dari jaringan epitel. Penelitian ini bertujuan untuk mengetahui profil tipe orang yang terdiagnosis kanker kolorektal di Rumah Sakit Pendidikan Ibnu Sina Makassar pada tahun 2022. Penelitian deskriptif observasional dilakukan. Total sampling digunakan untuk mengumpulkan sampel penelitian sebanyak 55 pasien, yang diambil dari populasi pasien kanker kolorektal di Rumah Sakit Pendidikan Ibnu Sina Makassar. Kriteria inklusi diperhitungkan selama pengumpulan sampel, yaitu pasien kanker kolorektal dengan data rekam medik lengkap. Data diolah dengan metode univariat. Mayoritas pasien kanker kolorektal berusia 46-55 tahun (38,2%), didominasi oleh laki-laki (52,7%), riwayat keluarga yaitu yang tidak memiliki riwayat keluarga (80,0%), tingkat pendidikan terbanyak SD (36,4%), jenis pekerjaan sebagai IRT (30,9%), berdasarkan suku yaitu makassar (58,2%), riwayat sering konsumsi daging merah dengan pasien yang memiliki riwayat (80,0%), riwayat merokok dengan pasien yang tidak memiliki riwayat (52,7%), lokasi tersering berada di rektum (69,1%), gambaran histopatologi adenokarsinoma (96,4%) dan jenis kemoterapi yaitu regimen FOLFOX (56,4%. Usia, jenis kelamin, riwayat keluarga, pendidikan, pekerjaan, suku, riwayat konsumsi daging merah, riwayat merokok, lokasi kanker, gambaran histopatologi serta jenis kemoterapi dari pasien kanker kolorektal merupakan hal yang perlu untuk diperhatikan.
Ekologizacja pojazdów samochodowych wchodzących w skład zestawów klas LHV i HCT-HCV – zasadnicze tendencje
Authors: Brach Jarosław
Published: March 2024

Jeden z technicznych sposobów na poprawę efektywności drogowych przewozów towarowych stanowi zezwolenie na poruszanie się jako nieponadgabarytowe przez zestawy dłuższe i cięższe. Ta koncepcja stanowiła bazę dla kategorii zestawów EMS – 25,25 m, 60 000 kg, a potem HCT-HCV i SEC, z zestawami 32–34‑metrowymi, 72–104‑tonowymi. Kolejne istotne wyzwanie stanowi ekologizacja takich dłuższych i cięższych zestawów, polegająca na stopniowym wprowadzaniu w nich paliw gazowych oraz zelektryfikowanych układów napędowych, co pozwala uzyskać dodatkowe korzyści środowiskowe. Zasadniczy cel tego artykułu stanowi analiza wprowadzania w zestawach klasy EMS 25,25 m oraz HCT/HCV i SEC paliw gazowych i elektryfikowanych układów napędowych – czy powyższe wykazuje już pewne uzasadnienie ekonomiczne i użytkowe. Artykuł zasadniczo powstał na podstawie analizy materiałów pochodzących od producentów odpowiedniego ekologicznego taboru oraz przedsiębiorstw eksploatujących ten tabor.
Perancangan Pop-Up Book sebagai Media Pengenalan Pengendalian Diri untuk Anak Usia 5-6 Tahun
Authors: Hariyanto Agnes Tea Kirana , Nirwana Aditya , Sukmaraga Ayyub Anshari
Published: March 2024

Indonesia yang dikenal dengan sifat ramahnya mulai mengalami beberapa gejala degradasi moral bahkan pada anak sekolah dasar. Hal ini didasari oleh beberapa faktor seperti paparan konten buruk serta kurangnya pengajaran pendidikan karakter. Konsep pengendalian diri sebagai ‘cangkang’ dari pendidikan karakter lainnya, sebaiknya diajarkan sejak dini agar dapat membedakan hal yang seharusnya dilakukan dan tidak dilakukan. Tujuan perancangan ini adalah mengenalkan pengendalian diri bagi anak-anak melalui media pembelajaran informal berupa buku cerita pop-up berjudul “Even Heroes Need Self-Control”. Untuk mencapai tujuan ini, perancang melakukan pengumpulan data secara kualitatif melalui observasi, wawancara, serta studi literatur yang digunakan sebagai acuan dari perancangan dengan metode Research and Development. Perwujudan akhir media utama berupa buku 14 halaman hardcover dengan media pendukung berupa secondary packaging, poster magnet interaktif, bucket hat, custom sanitizer, botol minum, serta enamel pin. Indikator capaian dari perancangan adalah keberhasilan mengenalkan pengendalian diri yang ditandai dengan penyebutan contoh pengendalian diri dalam kehidupan sehari-hari.
Pengaruh Karakteristik Orangtua terhadap Status Karies Gigi Anak Usia 5-6 Tahun di Kabupaten Maros Tahun 2018
Authors: Basri Nurfillah
Published: March 2024

Perilaku seseorang dapat dipengaruhi oleh peran orangtua, hal ini disebabkan karena orangtua adalah pusat kehidupan pertama anak. Orangtua bertanggung jawab memelihara, merawat, melindungi dan mendidik anak agar berkembang dengan baik, sehingga semakin tinggi tingkat pendidikan seseorang akan cenderung bertambah pula ilmu pengetahuan yang dimilikinya dan dapat mempengaruhi cara membimbing belajar anaknya. Begitupun dalam hal kesehatan gigi dan mulut anaknya, sudah seharusnya sejalan dengan ilmu pengetahuan yang dimiliki orangtuanya. Tujuan peneltian ini untuk menganalisis pengaruh karakteristik orangtua terhadap status karies gigi anak usia 5-6 tahun di Kabupaten Maros tahun 2018. Jenis penelitian ini adalah observasional analitik dengan menggunakan metode cross sectional study. Sampel berjumlah 220 orang anak usia 5-6 tahun. Hasil penelitian menunjukkan terdapat pengaruh pendidikan orangtua terhadap status karies gigi anak usia 5-6 tahun (p = 0, 006 < dari nilai α = 0,05), pengetahuan orangtua (p = 0,029 < dari nilai α = 0,05), sikap orangtua (p = 0,02 < dari nilai α = 0,05), tindakan orangtua (p = 0, 046 < dari nilai α = 0,05). Tidak terdapat pengaruh pekerjaan orangtua (p = 0, 403 > dari nilai α = 0,05) dan penghasilan orangtua (p = 0,473 > dari nilai α = 0,05). Simpulan yang paling berpengaruh adalah pendidikan (p=0,007 < dari α = 0,05). Saran orangtua tetap memperhatikan oral hygiene anaknya, serta bekerjasama dengan pemerintah setempat dalam mengurangi angka kejadian karies.

Kata kunci : Karakteristik orangtua, Karies gigi anak
PENINGKATAN BERAT BADAN BAYI 0 – 6 BULAN MELALUI TINDAKAN PEMIJATAN BAYI
Authors: Sari Lili Purnama , Diniaty Rosidi Indah Yun
Published: March 2024

Pijat bayi merupakan terapi sentuhan, stimulasi taktil yang memiliki keuntungan dalam proses tumbuh kembang bayi dan merupakan tradisi lama yang mulai diterapkan dalam ilmukesehatan. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemijatan bayi terhadap peningkatan berat badan bayi 0-6 bulan. Penelitian ini merupakan jenis penelitian Experiment dengan desain One Group Pretest-Postest Design. Pengambilan sampel dilakukan dengan teknik purposive sampling dan sampel sebanyak 30 responden. Instrument yang digunakan adalah lembar observasi. Data dianalisis menggunakan program SPSS dengan uji statistik Uji Paired Sample Test. Berdasarkan hasil analisis pengaruh pemijatan bayiterhadap peningkatan berat badan bayi diperoleh nilai ρ = 0,000 berarti ada pengaruh pemijatan bayi terhadap peingkatan berat badan bayi usia 0-6 bulan. Kesimpulan dalam penelitian ini adalah untuk kenaikan berat badan bayi yang signifikan maka pemijatan pada bayi harus dilakukan secara teratur yaitu minimal 2-3 kali dalam seminggu. Diharapkan adanya penelitian lebih lanjut dengan variabel penelitian yang lebih baik.
PENGARUH LATIHAN ROM AKTIF TERHADAP KELENTURAN SENDI EKSTREMITAS BAWAH PADA LANSIA DI PSTW KABUPATEN GOWA
Authors: Sari Sri Nurinda
Published: March 2024

Lansia merupakan proses yang fisiologis dimana akan mengalami kemunduran sistemorgan, yakni salah satunya sistem musculoskeletal sehingga lansia akan menyebabkan keterbatasangerak, sendi yang menahan berat badan lebih pada bagian ekstremitas bawah seperti sendi pinggul danseni lutut. Latihan Range of Motion (ROM) aktif adalah salah satu alternatif latihan rentang gerak yangdapat membantu lansia untuk memperbaiki sendinya.Tujuan penelitian ini membuktikan pengaruh latihan Range of Motion (ROM) aktif terhadapkelenturan sendi ekstremitas bawah pada lansia di Panti Sosial Tresna Werdha Gau Mabaji Kabupaten Gowa. Metode penelitian  menggunakan Pre-Eksperimen (One Group Pretest dan Posttest Design),dengan sampel sebanyak 20 lansia. Tehnik pengambilan sampel menggunakan purposive sampling, ujistatistik dalam penelitian ini menggunakan wiLcoxon.Hasil penelitian menunjukkan bahwa nilai t hitung 3,559   t tabel 2,093 sedangkan P value 0.002 α0,05 artinya terdapat pengaruh latihan ROM aktif terhadap kelenturan sendiekstremitas bawah pada lansia di Panti Sosial Tresna Werdha Gau Mabaji Kabupaten Gowa.Implikasi  penelitian ini bahwa latihan ROM aktif dapat melenturkan sendiekstremitas bawah pada lansia asalkan lansia mau melakukan latihan ROM secara teratur. Rekomendasi untuk pelayanan keperawatan terkhusus pada keperawatan gerontik agar mengajarkan latihan ROM aktif pada lansia supaya sendi lansia menjadi lebih baik
Construction of porphyrinic manganese-organic frameworks based on structural regulation for electrochemical determination of nitrobenzene in water and vegetable samples
Authors: Wang Li , Zhang Mengjie , Li Yuanyuan , Chen Xiumei , Qin Hao , Yang Jin , Fan Suhua , Wu Hai
Published: March 2024

Nitrobenzene (NB) is one of the major organic pollutants that has seriously endangered human health and the environment even in trace amounts. Therefore, it is of great significance to detect trace NB efficiently and sensitively. Herein, a porphyrinic metal-organic framework (MOF) of Mn-PCN-222 (PCN, porous coordination network) was first synthesized by the coordination between Zr6 cluster and tetrakis (4-carboxyphenyl)-porphyrin-Mn (Ⅲ) (MnTCPPCl) ligand. To regulate its structure and the electrochemical properties, a phenyl group was inserted in each branched chain of TCPP to form the TCBPP organic ligand. Then, we used Zr6 clusters and manganese metalloporphyrin (MnTCBPPCl) to synthesize a new porphyrin-based MOF (Mn-CPM-99, CPM, crystalline porous material). Due to the extended chains of TCPP, the rod-shaped structure of Mn-PCN-222 was switched to concave quadrangular bipyramid of Mn-CPM-99. Mn-CPM-99 exhibited higher porosity, larger specific surface area, better electrochemical performances than those of Mn-PCN-222. By using modular assembly technique, Mn-CPM-99 film was sequentially assembled on the surface of indium-tin-oxide (ITO) to prepare an electrochemical sensor (Mn-CPM-99/ITO). The proposed sensor showed excellent electrochemical reduction of NB and displayed three linear response ranges in the wide concentration ranges. The obtained low limit of detection (LOD, 1.3 nM), high sensitivity and selectivity, and good reproducibility of the sensor for NB detection fully illustrate that Mn-CPM-99 is an excellent candidate for electrochemical sensor interface material. Moreover, the sensor was successfully applied to the detection of NB in lake water and vegetable samples showing satisfactory recovery of 98.9%–101.8%.
Cell immunity to SARS-CoV-2 after natural infection and/or different vaccination regimens
Authors: Culebras Esther , Martínez Mercedes , Novella Consuelo , León Jose Manuel , Marcos Esther , Delgado-Iribarren Alberto , Ríos Esther
Published: March 2024

Background

The aim of the study was to evaluate the humoral and cellular immunity after SARS-CoV-2 infection and/or vaccination according to the type of vaccine, number of doses and combination of vaccines.

Methods

Volunteer subjects were sampled between September 2021 and July 2022 in Hospital Clínico San Carlos, Madrid (Spain). Participants had different immunological status against SARS-CoV-2: vaccinated and unvaccinated, with or without previous COVID-19 infection, including healthy and immunocompromised individuals. Determination of IgG against the spike protein S1 subunit receptor-binding domain (RBD) was performed by chemiluminescence microparticle immunoassay (CMIA) using the Architect i10000sr platform (Abbott). The SARS-CoV-2-specific T-cell responses were assessed by quantification of interferon gamma release using QuantiFERON SARS-CoV-2 assay (Qiagen).

Results

A total of 181 samples were collected, 170 were from vaccinated individuals and 11 from unvaccinated. Among the participants, 41 were aware of having previously been infected by SARS-CoV-2. Vaccinated people received one or two doses of the following vaccines against SARS-CoV-2: ChAdOx1-S (University of Oxford—AstraZeneca) (AZ) and/orBNT162b2 (Pfizer—BioNTech)(PZ). Subjects immunized with a third-booster dose received PZ or mRNA-1273 (Moderna—NIAID)(MD) vaccines. All vaccinees developed a positive humoral response (>7.1 BAU/ml), but the cellular response varied depending on the vaccination regimen. Only AZ/PZ combination and 3 doses of vaccination elicited a positive cellular response (median concentration of IFN- γ > 0.3 IU/ml). Regarding a two-dose vaccination regimen, AZ/PZ combination induced the highest humoral and cellular immunity. A booster with mRNA vaccine resulted in increases in median levels of IgG-Spike antibodies and IFN-γ as compared to those of two-dose of any vaccine. Humoral and cellular immunity levels were significantly higher in participants with previous infection compared to those without infection.

Conclusion

Heterologous vaccination (AZ/PZ) elicited the strongest immunity among the two-dose vaccination regimens. The immunity offered by the third-booster dose of SARS-CoV-2 vaccine depends not only on the type of vaccine administered but also on previous doses and prior infection. Previous exposure to SARS-CoV-2 antigens by infection strongly affect immunity of vaccinated individuals.
The effects of prebiotic, probiotic or synbiotic supplementation on overweight/obesity indicators: an umbrella review of the trials’ meta-analyses
Authors: Rasaei Niloufar , Heidari Mohammadreza , Esmaeili Fataneh , Khosravi Sepehr , Baeeri Maryam , Tabatabaei-Malazy Ozra , Emamgholipour Solaleh
Published: March 2024

Background

There is controversial data on the effects of prebiotic, probiotic, or synbiotic supplementations on overweight/obesity indicators. Thus, we aimed to clarify this role of biotics through an umbrella review of the trials’ meta-analyses.

Methods

All meta-analyses of the clinical trials conducted on the impact of biotics on overweight/obesity indicators in general populations, pregnant women, and infants published until June 2023 in PubMed, Web of Sciences, Scopus, Embase, and Cochrane Library web databases included. The meta-analysis of observational and systematic review studies without meta-analysis were excluded. We reported the results by implementing the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) flowchart. The Assessment of Multiple Systematic Reviews-2 (AMSTAR2) and Grading of Recommendations Assessment, Development, and Evaluation (GRADE) systems were used to assess the methodological quality and quality of evidence.

Results

Overall, 97 meta-analysis studies were included. Most studies were conducted on the effect of probiotics in both genders. Consumption of prebiotic: 8-66 g/day, probiotic: 104 -1.35×1015 colony-forming unit (CFU)/day, and synbiotic: 106-1.5×1011 CFU/day and 0.5-300 g/day for 2 to 104 weeks showed a favorable effect on the overweight/obesity indicators. Moreover, an inverse association was observed between biotics consumption and overweight/obesity risk in adults in most of the studies. Biotics did not show any beneficial effect on weight and body mass index (BMI) in pregnant women by 6.6×105-1010 CFU/day of probiotics during 1-25 weeks and 1×109-112.5×109 CFU/capsule of synbiotics during 4-8 weeks. The effect of biotics on weight and BMI in infants is predominantly non-significant. Prebiotics and probiotics used in infancy were from 0.15 to 0.8 g/dL and 2×106-6×109 CFU/day for 2-24 weeks, respectively.

Conclusion

It seems biotics consumption can result in favorable impacts on some anthropometric indices of overweight/obesity (body weight, BMI, waist circumference) in the general population, without any significant effects on birth weight or weight gain during pregnancy and infancy. So, it is recommended to intake the biotics as complementary medications for reducing anthropometric indices of overweight/obese adults. However, more well-designed trials are needed to elucidate the anti-obesity effects of specific strains of probiotics.
Lifestyle and fertility-specific quality of life affect reproductive outcomes in couples undergoing in vitro fertilization
Authors: Urata Yoko , Harada Miyuki , Komiya Shinnosuke , Akiyama Ikumi , Tuchida Chihiro , Nakaoka Yoshiharu , Fukuda Aisaku , Morimoto Yoshiharu , Kawahara Takuya , Ishikawa Yusuke , Osuga Yutaka
Published: March 2024

Objective

A Mediterranean dietary pattern, sleeping habits, physical activity, and lifestyle appear to affect reproductive health. There are few reports about whether fertility-specific quality of life (QOL) is linked to infertility treatment outcomes. The aim of this study is to investigate when lifestyle factors and fertility-specific QOL are comprehensively considered, which factors influence assisted reproductive technology (ART) outcomes.

Methods

This prospective cohort includes 291 women undergoing a first ART treatment at multiple centers in Japan and was designed to evaluate the influence of diet, physical activity, sleeping pattern, computer use duration, and fertility-specific quality of life tool (FertiQoL) score on ART treatment outcomes using a questionnaire. The primary endpoint was the good-quality blastocyst rate per oocyte retrieval and the secondary endpoints were a positive pregnancy test and gestational sac (GS) detection.

Results

The good-quality blastocyst rate per oocyte retrieval tended to be negatively associated with frequent fish consumption. After all embryo transfer (ET) cycles, a positive pregnancy test tended to be positively associated with longer sleep and longer computer use (OR = 1.6, 95% CI = 0.9–2.7 and OR = 1.7, CI = 1.0–2.8, respectively) and negatively associated with a smoking partner (OR = 0.6, CI = 0.3–1.0). GS detection was positively and significantly associated with frequent olive oil intake and longer computer use (OR = 1.7, CI = 1.0–3.0 and OR = 1.7, CI = 1.0–3.0, respectively). After ET cycles with a single blastocyst, a positive pregnancy test was positively and significantly associated with longer computer use (OR = 2.0, CI = 1.1–3.7), while GS detection was significantly more likely in women with longer computer use (OR = 2.1, CI = 1.1–3.8) and tended to be more likely in women with a higher FertiQoL Total scaled treatment score (OR = 1.8, CI = 1.0–3.3). p < 0.05 was considered statistically significant and 0.05 ≤ p <0.01 as tendency.

Conclusions

Olive oil may be an important factor in dietary habits. Fertility-specific QOL and smoking cessation guidance for partners are important for infertile couples.
XR and digital twins, and their role in human factor studies
Authors: Roy Samrendra , Singh Suneet , Rizwan-uddin
Published: March 2024

Digital twins (DT) are synchronized clones that mirror their physical counterparts at all times, enabling real-time monitoring, analysis, decision-making, and planning for optimized operations. Digital twins can transcend traditional two-dimensional interfaces by incorporating VR/AR/MR (XR) technology, providing immersive, intuitive, and interactive representations of systems, assets, and processes. Furthermore, real-time data from sensors, simulations, and other sources can be integrated into the XR-enabled digital twins, leading to better and more intuitive understanding and to more efficiently monitor, analyze, and maintain complex systems such as nuclear assets. Immersive, interactive, multi-player XR capabilities embedded in DTs will allow spatially accurate and more realistic representation, leading to improved risk assessment, optimized and predictive maintenance scheduling, enhanced situational awareness, and more effective communication among interdisciplinary teams. By combining the strengths of XR and digital twins, nuclear facilities can achieve heightened safety, operational efficiency, and decision-making accuracy. Marrying XR technology with digital twins is also likely to extend the utilization of digital twins to optimize those aspects of the design and operation of nuclear assets that involve human beings–specifically in human factors engineering. Training can also be significantly enhanced if DTs are linked with XR technology. These systems may also be used to assess human performance through human factors engineering for the safety analysis of nuclear assets. A specific example is assessing human performance in semi-autonomous nuclear assets or operating multiple nuclear assets. After briefly reviewing digital twins of nuclear systems from the perspective of XR technology, this paper summarises our work in the nuclear energy space on VR/AR/MR and how these can be integrated into the newly evolving DTs of nuclear assets. The paper also describes the potential use of such systems in optimizing the design and operations of nuclear systems. As XR technology advances, its symbiotic relationship with digital twins can significantly reshape the landscape of nuclear operations and asset management.
Toward reproducible tumor organoid culture: focusing on primary liver cancer
Authors: Guo Lianming , Li Chao , Gong Weiqiang
Published: March 2024

Organoids present substantial potential for pushing forward preclinical research and personalized medicine by accurately recapitulating tissue and tumor heterogeneity in vitro. However, the lack of standardized protocols for cancer organoid culture has hindered reproducibility. This paper comprehensively reviews the current challenges associated with cancer organoid culture and highlights recent multidisciplinary advancements in the field with a specific focus on standardizing liver cancer organoid culture. We discuss the non-standardized aspects, including tissue sources, processing techniques, medium formulations, and matrix materials, that contribute to technical variability. Furthermore, we emphasize the need to establish reproducible platforms that accurately preserve the genetic, proteomic, morphological, and pharmacotypic features of the parent tumor. At the end of each section, our focus shifts to organoid culture standardization in primary liver cancer. By addressing these challenges, we can enhance the reproducibility and clinical translation of cancer organoid systems, enabling their potential applications in precision medicine, drug screening, and preclinical research.
Predicting immunotherapy response in melanoma using a novel tumor immunological phenotype-related gene index
Authors: Zheng Shaoluan , He Anqi , Chen Chenxi , Gu Jianying , Wei Chuanyuan , Chen Zhiwei , Liu Jiaqi
Published: March 2024

Introduction

Melanoma is a highly aggressive and recurrent form of skin cancer, posing challenges in prognosis and therapy prediction.

Methods

In this study, we developed a novel TIPRGPI consisting of 20 genes using Univariate Cox regression and the LASSO algorithm. The high and low-risk groups based on TIPRGPI exhibited distinct mutation profiles, hallmark pathways, and immune cell infiltration in the tumor microenvironment.

Results

Notably, significant differences in tumor immunogenicity and TIDE were observed between the risk groups, suggesting a better response to immune checkpoint blockade therapy in the low-TIPRGPI group. Additionally, molecular docking predicted 10 potential drugs that bind to the core target, PTPRC, of the TIPRGPI signature.

Discussion

Our findings highlight the reliability of TIPRGPI as a prognostic signature and its potential application in risk classification, immunotherapy response prediction, and drug candidate identification for melanoma treatment. The "TIP genes" guided strategy presented in this study may have implications beyond melanoma and could be applied to other cancer types.
Suppression of the growth and metastasis of mouse melanoma by Taenia crassiceps and Mesocestoides corti tapeworms
Authors: Schreiber Manfred , Macháček Tomáš , Vajs Vojtěch , Šmídová Barbora , Majer Martin , Hrdý Jiří , Tolde Ondřej , Brábek Jan , Rösel Daniel , Horák Petr
Published: March 2024

Cancer is still one of the leading causes of death, with an estimated 19.3 million new cases every year. Our paper presents the tumor-suppressing effect of Taenia crassiceps and Mesocestoides corti on B16F10 melanoma, the intraperitoneal application of which followed the experimental infection with these tapeworms, resulting in varying degrees of effectiveness in two strains of mice. In the case of M. corti-infected ICR mice, a strong tumor growth suppression occurred, which was accompanied by a significant reduction in the formation of distant metastases in the liver and lung. Tapeworm-infected C57BL/6J mice also showed a suppression of tumor growth and, in addition, the overall survival of infected C57BL/6J mice was significantly improved. Experiments with potential cross-reaction of melanoma and tapeworm antigens with respective specific antibodies, restimulation of spleen T cells, or the direct effect of tapeworm excretory-secretory products on melanoma cells in vitro could not explain the phenomenon. However, infections with T. crassiceps and M. corti increased the number of leukocytes possibly involved in anti-tumor immunity in the peritoneal cavity of both ICR and C57BL/6J mice. This study unveils the complex interplay between tapeworm infections, immune responses, and melanoma progression, emphasizing the need for further exploration of the mechanisms driving observed tumor-suppressive effects.
Association between vascular FDG uptake during follow-up and the development of thoracic aortic aneurysms in giant cell arteritis
Authors: Blockmans Daniel , Moreel Lien , Betrains Albrecht , Vanderschueren Steven , Coudyzer Walter , Boeckxstaens Lennert , Van Laere Koen
Published: March 2024

Background

A positive PET scan at diagnosis was associated with a greater yearly increase in ascending and descending aortic diameter and thoracic aortic volume in patients with giant cell arteritis (GCA). Radiologic and histopathologic vascular abnormalities persist in a subset of treated patients despite clinical remission. The aim of this study was to evaluate the association between vascular FDG uptake during follow-up and the development of thoracic aortic aneurysms.

Methods

We recently performed a prospective cohort study of 106 GCA patients, who underwent FDG PET and CT imaging at diagnosis and CT imaging yearly for a maximum of 10 years. In this post hoc analysis, GCA patients who also have had FDG PET imaging during follow-up were included. PET scans were visually scored (0–3) at 7 vascular areas. PET scans were considered positive in case of FDG uptake ≥grade 2 in any large vessel.

Results

Eighty-eight repeat PET scans were performed in 52 out of 106 GCA patients, who were included in the original prospective cohort. Fifty-five (63%) PET scans were done at the time of a relapse and 33 (38%) were done while in remission. Nine out of ten patients with an incident thoracic aortic aneurysm had both a positive PET scan at diagnosis and during follow-up.

Conclusion

In addition to the intensity and extent of the initial vascular inflammation, ongoing aortic inflammation may contribute to the development of thoracic aortic aneurysms in GCA. However, this hypothesis should be confirmed in a large prospective trial with repeat PET scans at predefined time points during follow-up.
CGRWDL: alignment-free phylogeny reconstruction method for viruses based on chaos game representation weighted by dynamical language model
Authors: Wang Ting , Yu Zu-Guo , Li Jinyan
Published: March 2024

Traditional alignment-based methods meet serious challenges in genome sequence comparison and phylogeny reconstruction due to their high computational complexity. Here, we propose a new alignment-free method to analyze the phylogenetic relationships (classification) among species. In our method, the dynamical language (DL) model and the chaos game representation (CGR) method are used to characterize the frequency information and the context information of k-mers in a sequence, respectively. Then for each DNA sequence or protein sequence in a dataset, our method converts the sequence into a feature vector that represents the sequence information based on CGR weighted by the DL model to infer phylogenetic relationships. We name our method CGRWDL. Its performance was tested on both DNA and protein sequences of 8 datasets of viruses to construct the phylogenetic trees. We compared the Robinson-Foulds (RF) distance between the phylogenetic tree constructed by CGRWDL and the reference tree by other advanced methods for each dataset. The results show that the phylogenetic trees constructed by CGRWDL can accurately classify the viruses, and the RF scores between the trees and the reference trees are smaller than that with other methods.
The association between plasma IgG N-glycosylation and neonatal hypoxic–ischemic encephalopathy: a case-control study
Authors: Wang Liangao , Lu Xinxia , Wang Meng , Zhao Xuezhen , Li Peirui , Zhang Haitao , Meng Qingtang , Zhang Yujing , Wang Yingjie , Wang Wei , Ji Long , Hou Haifeng , Li Dong
Published: March 2024

Introduction

Hypoxic-ischemic encephalopathy (HIE) is one of severe neonatal brain injuries, resulting from inflammation and the immune response after perinatal hypoxia and ischemia. IgG N-glycosylation plays a crucial role in various inflammatory diseases through mediating the balance between anti-inflammatory and pro-inflammatory responses. This study aimed to explore the effect of IgG N-glycosylation on the development of HIE.

Methods

This case-control study included 53 HIE patients and 57 control neonates. An ultrahigh-performance liquid chromatography (UPLC) method was used to determine the features of the plasma IgG N-glycans, by which 24 initial glycan peaks (GPs) were quantified. Multivariate logistic regression was used to examine the association between initial glycans and HIE, by which the significant parameters were used to develop a diagnostic model. Though receiver operating characteristic (ROC) curves, area under the curve (AUC) and 95% confidence interval (CI) were calculated to assess the performance of the diagnostic model.

Results

There were significant differences in 11 initial glycans between the patient and control groups. The levels of fucosylated and galactosylated glycans were significantly lower in HIE patients than in control individuals, while sialylated glycans were higher in HIE patients (p < 0.05). A prediction model was developed using three initial IgG N-glycans and fetal distress, low birth weight, and globulin. The ROC analysis showed that this model was able to discriminate between HIE patients and healthy individuals [AUC = 0.798, 95% CI: (0.716–0.880)].

Discussion

IgG N-glycosylation may play a role in the pathogenesis of HIE. Plasma IgG N-glycans are potential noninvasive biomarkers for screening individuals at high risk of HIE.
Nanoparticle-based optical interfaces for retinal neuromodulation: a review
Authors: Stoddart Paul R. , Begeng James M. , Tong Wei , Ibbotson Michael R. , Kameneva Tatiana
Published: March 2024

Degeneration of photoreceptors in the retina is a leading cause of blindness, but commonly leaves the retinal ganglion cells (RGCs) and/or bipolar cells extant. Consequently, these cells are an attractive target for the invasive electrical implants colloquially known as “bionic eyes.” However, after more than two decades of concerted effort, interfaces based on conventional electrical stimulation approaches have delivered limited efficacy, primarily due to the current spread in retinal tissue, which precludes high-acuity vision. The ideal prosthetic solution would be less invasive, provide single-cell resolution and an ability to differentiate between different cell types. Nanoparticle-mediated approaches can address some of these requirements, with particular attention being directed at light-sensitive nanoparticles that can be accessed via the intrinsic optics of the eye. Here we survey the available known nanoparticle-based optical transduction mechanisms that can be exploited for neuromodulation. We review the rapid progress in the field, together with outstanding challenges that must be addressed to translate these techniques to clinical practice. In particular, successful translation will likely require efficient delivery of nanoparticles to stable and precisely defined locations in the retinal tissues. Therefore, we also emphasize the current literature relating to the pharmacokinetics of nanoparticles in the eye. While considerable challenges remain to be overcome, progress to date shows great potential for nanoparticle-based interfaces to revolutionize the field of visual prostheses.
Chromosomal localization of mutated genes in non-syndromic familial thyroid cancer
Authors: Jiang Yu-jia , Xia Yun , Han Zhuo-jun , Hu Yi-xuan , Huang Tao
Published: March 2024

Familial non-medullary thyroid carcinoma (FNMTC) is a type of thyroid cancer characterized by genetic susceptibility, representing approximately 5% of all non-medullary thyroid carcinomas. While some cases of FNMTC are associated with familial multi-organ tumor predisposition syndromes, the majority occur independently. The genetic mechanisms underlying non-syndromic FNMTC remain unclear. Initial studies utilized SNP linkage analysis to identify susceptibility loci, including the 1q21 locus, 2q21 locus, and 4q32 locus, among others. Subsequent research employed more advanced techniques such as Genome-wide Association Study and Whole Exome Sequencing, leading to the discovery of genes such as IMMP2L, GALNTL4, WDR11-AS1, DUOX2, NOP53, MAP2K5, and others. But FNMTC exhibits strong genetic heterogeneity, with each family having its own pathogenic genes. This is the first article to provide a chromosomal landscape map of susceptibility genes associated with non-syndromic FNMTC and analyze their potential associations. It also presents a detailed summary of variant loci, characteristics, research methodologies, and validation results from different countries.
Preoperative systemic immune-inflammation index-based nomogram for lung carcinoma following microwave ablation -a real world single center study
Authors: Wang Jing , Cui Song-ping , Zhao Qing , Gao Ya , Ji Ying , Liu Yi , Miao Jin-bai , Fu Yi-li , Hu Bin
Published: March 2024

Background

The preoperative inflammatory condition significantly influences the prognosis of malignancies. We aimed to investigate the potential significance of preoperative inflammatory biomarkers in forecasting the long-term results of lung carcinoma after microwave ablation (MWA).

Method

This study included patients who received MWA treatment for lung carcinoma from Jan. 2012 to Dec. 2020. We collected demographic, clinical, laboratory, and outcome information. To assess the predictive capacity of inflammatory biomarkers, we utilized the area under the receiver operating characteristic curve (AUC-ROC) and assessed the predictive potential of inflammatory biomarkers in forecasting outcomes through both univariate and multivariate Cox proportional hazard analyses.

Results

A total of 354 individuals underwent MWA treatment, of which 265 cases were included in this study, whose average age was 69.1 ± 9.7 years. The AUC values for the Systemic Inflammatory Response Index (SIRI) to overall survival (OS) and disease-free survival (DFS) were 0.796 and 0.716, respectively. The Cox proportional hazards model demonstrated a significant independent association between a high SIRI and a decreased overall survival (hazard ratio [HR]=2.583, P<0.001). Furthermore, a high SIRI independently correlated with a lower DFS (HR=2.391, P<0.001). We developed nomograms utilizing various independent factors to forecast the extended prognosis of patients. These nomograms exhibited AUC of 0.900, 0.849, and 0.862 for predicting 1-year, 3-year, and 5-year OS, respectively. Additionally, the AUC values for predicting 1-year, 3-year, and 5-year DFS were 0.851, 0.873, and 0.883, respectively.

Conclusion

SIRI has shown promise as a valuable long-term prognostic indicator for forecasting the outcomes of lung carcinoma patients following MWA.
Inflammation as a driver of hematological malignancies
Authors: Saluja Sumedha , Bansal Ishu , Bhardwaj Ruchi , Beg Mohammad Sabique , Palanichamy Jayanth Kumar
Published: March 2024

Hematopoiesis is a tightly regulated process that produces all adult blood cells and immune cells from multipotent hematopoietic stem cells (HSCs). HSCs usually remain quiescent, and in the presence of external stimuli like infection or inflammation, they undergo division and differentiation as a compensatory mechanism. Normal hematopoiesis is impacted by systemic inflammation, which causes HSCs to transition from quiescence to emergency myelopoiesis. At the molecular level, inflammatory cytokine signaling molecules such as tumor necrosis factor (TNF), interferons, interleukins, and toll-like receptors can all cause HSCs to multiply directly. These cytokines actively encourage HSC activation, proliferation, and differentiation during inflammation, which results in the generation and activation of immune cells required to combat acute injury. The bone marrow niche provides numerous soluble and stromal cell signals, which are essential for maintaining normal homeostasis and output of the bone marrow cells. Inflammatory signals also impact this bone marrow microenvironment called the HSC niche to regulate the inflammatory-induced hematopoiesis. Continuous pro-inflammatory cytokine and chemokine activation can have detrimental effects on the hematopoietic system, which can lead to cancer development, HSC depletion, and bone marrow failure. Reactive oxygen species (ROS), which damage DNA and ultimately lead to the transformation of HSCs into cancerous cells, are produced due to chronic inflammation. The biological elements of the HSC niche produce pro-inflammatory cytokines that cause clonal growth and the development of leukemic stem cells (LSCs) in hematological malignancies. The processes underlying how inflammation affects hematological malignancies are still not fully understood. In this review, we emphasize the effects of inflammation on normal hematopoiesis, the part it plays in the development and progression of hematological malignancies, and potential therapeutic applications for targeting these pathways for therapy in hematological malignancies.
Bone marrow adipocytes and lung cancer bone metastasis: unraveling the role of adipokines in the tumor microenvironment
Authors: Li Jian , Wu Jialu , Xie Yanni , Yu Xijie
Published: March 2024

Bone is a common site of metastasis for lung cancer. The “seed and soil” hypothesis suggests that the bone marrow microenvironment (“soil”) may provide a conducive survival environment for metastasizing tumor cells (“seeds”). The bone marrow microenvironment, comprising a complex array of cells, includes bone marrow adipocytes (BMAs), which constitute about 70% of the adult bone marrow volume and may play a significant role in tumor bone metastasis. BMAs can directly provide energy for tumor cells, promoting their proliferation and migration. Furthermore, BMAs participate in the tumor microenvironment’s osteogenesis regulation, osteoclast(OC) regulation, and immune response through the secretion of adipokines, cytokines, and inflammatory factors. However, the precise mechanisms of BMAs in lung cancer bone metastasis remain largely unclear. This review primarily explores the role of BMAs and their secreted adipokines (leptin, adiponectin, Nesfatin-1, Resistin, chemerin, visfatin) in lung cancer bone metastasis, aiming to provide new insights into the mechanisms and clinical treatment of lung cancer bone metastasis.
Advancements in the diagnosis and management of premature ventricular contractions in pediatric patients
Authors: Zhu Wenjing , Yuan Hui , Lv Jianli
Published: March 2024

Background

Premature ventricular contractions (PVCs) are relatively common arrhythmias in the pediatric population, with implications that range from benign to potentially life-threatening. The management of PVCs in children poses unique challenges, and recent advancements in diagnostic and therapeutic options call for a comprehensive review of current practices.

Methods

This review synthesizes the latest literature on pediatric PVCs, focusing on publications from the past decade. We evaluate studies addressing the epidemiology, pathophysiology, diagnosis, and treatment of PVCs in children, including pharmacological, non-pharmacological, and invasive strategies.

Results

The review identifies key advancements in the non-invasive detection of PVCs, the growing understanding of their genetic underpinnings, and the evolving landscape of management options. We discuss the clinical decision-making process, considering the variable significance of PVCs in different pediatric patient subgroups, and highlight the importance of individualized care. Current guidelines and consensus statements are examined, and areas of controversy or limited evidence are identified.

Conclusions

Our review underscores the need for a nuanced approach to PVCs in children, integrating the latest diagnostic techniques with a tailored therapeutic strategy. We call for further research into long-term outcomes and the development of risk stratification tools to guide treatment. The potential of emerging technologies and the importance of multidisciplinary care are also emphasized to improve prognoses for pediatric patients with PVCs.