bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into the Root Sprouts of Toona fargesii in a Natural Forest: From the Morphology, Physiology, and Transcriptome Levels
Authors: Cheng Qiangqiang , Ma Jikai , Guo Chunce , Zhong Qiuwei , Yu Wanwen , Jia Ting , Zhang Lu
Published: February 2024

Toona fargesii (T. fargesii) is a deciduous tree of the Meliaceae family which is utilized for high-value timber. Interestingly, root sprouting is a typical reproductive pattern in T. fargesii. Nevertheless, the genetics underlying this phenomenon are still unclear. Here, three type of roots of T. fargesii were used for histological observation, plant endogenous hormone determination, non-structural carbohydrate (NSC) determination, and sequencing using the Illumina next-generation and PacBio SMRT platforms. Our results indicated that root sprouts originated from cork cambiums. Furthermore, indole-3-acetic acid (IAA), zeatin riboside (ZR), gibberellic acid 3 (GA3), and abscisic acid (ABA) content was significantly increased, while soluble sugar content was significantly decreased in the root sprouts. In addition, transcriptomic analysis suggested a total of 36.19 G of raw data from which 210 differentially expressed genes (DEGs) of were identified in RS vs. SR. Of these, the candidate DEGs were largely enriched in the citrate cycle, gluconeogenesis, starch and sucrose metabolism, and plant hormone signal transduction pathways. We therefore speculated that the accumulation of cytokinin and auxin might be induced by ATP-binding cassette-B 19 (ABCB19) and ABCG14, which were necessary for root sprouting. Additionally, transcription factors SQUAMOSA promoter binding protein-like 18 (SPL18) and NAM, ATAF1/2, and CUC2-14 (NAC14) were found in response to environmental water and epigenetic modification in RS. Overall, this study was to unravel the physiological and transcriptomic levels of the development of root sprouting in T. fargesii.
Mechanical Properties of Furniture Joints Using Loose Tenons and Connectors
Authors: Bas Seda , Denes Levente , Csiha Csilla
Published: February 2024

The investigation conducted in this study focused on assessing the withdrawal resistance of T-joints and the bending moment capacity in the tension and compression of corner joints. For samples, preparation glued-in loose tenons (Domino dowels) and dismountable connectors were used as connecting elements. The joints were made of European beech wood and a D3-grade PVAc adhesive was utilized for bonding. The effect of the joint type, the shoulders’ bonding, and the load application direction were investigated. The test results revealed that the withdrawal resistance of Domino dowel joints exhibited twice the strength compared to Domino connectors. Moreover, the presence of a bonded area on the shoulders did not significantly impact the strength of the joints. In the case of corner joints, the bending moment capacity in compression was notably influenced by the bond line on the shoulders, although such an effect was not significant in tension. Domino dowel joints provided a robust and reliable permanent connection between wooden elements, surpassing Domino connectors; however, in both cases, the strength values exceeded those of conventional dowels and cam lock connector joints.
Comparative Analysis of Simulation Methodologies for Spindle Pumps
Authors: Borriello Pasquale , Frosina Emma , Lucchesi Pierpaolo , Senatore Adolfo
Published: February 2024

This research conducts a comprehensive comparative analysis of simulation methodologies for spindle pumps, with a specific focus on steady-state CFD, transient-CFD, and lumped-parameter approaches. Spindle pumps, renowned for their reliability, efficiency, and low noise emission, play a pivotal role in Thermal Management for Battery Electric Vehicles, aligning with the automotive industry’s commitment to reducing pollutants and CO2 emissions. The study is motivated by the critical need to curtail energy consumption during on-the-road operations, particularly as the automotive industry strives for enhanced efficiency. While centrifugal pumps are commonly employed for such applications, their efficiency is highly contingent on rotational speed, leading to energy wastage in real-world scenarios despite high efficiency at the design point. Consequently, the adoption of precisely designed spindle pumps for thermal management systems emerges as a viable solution to meet evolving industry needs. Recognizing the profound impact of simulation tools on the design and optimization phases for pump manufacturers, this research emphasizes the significance of fast and accurate simulation tools. Transient-CFD emerges as a powerful Tool, enabling real-time monitoring of various performance indicators, while steady-CFD, with minimal simplifications, adeptly captures pressure distribution and machine leakages. Lumped-parameter approaches, though requiring effort in simulation setup and simplifying input geometry, offer rapid computational times and comprehensive predictions, including leakages, Torque, cavitation, and pressure ripple. Breaking new ground, this paper presents, for the first time in the literature, accurate simulation models for the same reference machine using the aforementioned methodologies. The results were rigorously validated against experiments spanning a wide range of pump speeds and pressure drops. The discussion encompasses predicted flow, Torque, cavitation, and pressure ripple, offering valuable insights into the strengths and limitations of each methodology.
A Novel Bacillus Velezensis for Efficient Degradation of Zearalenone
Authors: Li Yijia , Chen Songbiao , Yu Zuhua , Yao Jie , Jia Yanyan , Liao Chengshui , Chen Jian , Wei Ying , Guo Rongxian , He Lei , Ding Ke
Published: February 2024

Zearalenone (ZEN) is considered one of the most serious mycotoxins contaminating grains and their by-products, causing significant economic losses in the feed and food industries. Biodegradation pathways are currently considered the most efficient solution to remove ZEN contamination from foods. However, low degradation rates and vulnerability to environmental impacts limit the application of biodegradation pathways. Therefore, the main research objective of this article was to screen strains that can efficiently degrade ZEN and survive under harsh conditions. This study successfully isolated a new strain L9 which can efficiently degrade ZEN from 108 food ingredients. The results of sequence alignment showed that L9 is Bacillus velezensis. Meanwhile, we found that the L9 degradation rate reached 91.14% at 24 h and confirmed that the primary degradation mechanism of this strain is biodegradation. The strain exhibits resistance to high temperature, acid, and 0.3% bile salts. The results of whole-genome sequencing analysis showed that, it is possible that the strain encodes the key enzyme, such as chitinase, carboxylesterases, and lactone hydrolase, that work together to degrade ZEN. In addition, 227 unique genes in this strain are primarily involved in its replication, recombination, repair, and protective mechanisms. In summary, we successfully excavated a ZEN-degrading, genetically distinct strain of Bacillus velezensis that provides a solid foundation for the detoxification of feed and food contamination in the natural environment.
Baccharis dracunculifolia DC Consumption Improves Nociceptive and Depressive-like Behavior in Rats with Experimental Osteoarthritis
Authors: Laranjeira Inês Martins , Apolinário Elisabete , Amorim Diana , da Silva Filho Ademar Alves , Dias Alberto Carlos Pires , Pinto-Ribeiro Filipa
Published: February 2024

Osteoarthritis (OA) persistently activates nociceptors, leading to chronic pain, which is often accompanied by the comorbid development of emotional impairments (anxiety and depression), an effect associated with microgliosis. Baccharis dracunculifolia DC (Asteraceae), a Brazilian edible plant, is an important source of active compounds with anti-inflammatory abilities. Thus, we evaluated its ability to reverse OA-induced nociceptive and emotional-like impairments in osteoarthritic ovariectomized female rats using the kaolin/carrageenan (K/C) model. Four weeks after OA induction, mechanical hyperalgesia was confirmed, and the treatment started. Control animals (SHAMs) were treated with phosphate-buffered saline (PBS), while arthritic animals (ARTHs) either received PBS or B. dracunculifolia 50 mg/kg (Bd50) and 100 mg/kg (Bd100), via gavage, daily for five weeks. At the end of the treatment, anxiety-like behavior was assessed using the Open Field Test (OFT), anhedonia was assessed using the Sucrose Preference Test (SPT), and learned helplessness was assessed using the Forced Swimming Test (FST). After occision, microglia were stained with IBA-1 and quantified in brain sections of target areas (prefrontal cortex, amygdala, and periaqueductal grey matter). Treatment with B. dracunculifolia extract reversed OA-induced mechanical hyperalgesia and partly improved depressive-like behavior in OA animals’ concomitant to a decrease in the number of M1 microglia. Our findings suggest that B. dracunculifolia extracts can potentially be used in the food industry and for the development of nutraceuticals and functional foods.
Fucus vesiculosus-Rich Extracts as Potential Functional Food Ingredients: A Holistic Extraction Approach
Authors: Circuncisão Ana R. , Ferreira Sónia S. , Silva Artur M. S. , Coimbra Manuel A. , Cardoso Susana M.
Published: February 2024

Brown macroalgae are rich sources of nutrients and health-promoting compounds. Nevertheless, their consumption is still limited by their strong organoleptic characteristics, thus requiring the development of extraction strategies to profit from their nutritional value. To fulfil this, two sequential extraction approaches were developed, differing in the solvent used in the first extraction step, water in approach 1 or food-grade ethanol in approach 2, to obtain economic and affordable extracts rich in specific compounds from Fucus vesiculosus. The use of water in the first step of extraction allowed us to recover water-soluble phlorotannins, laminarans and mannuronic-rich alginates, making the subsequent 70% ethanol extract richest in fucoxanthin (0.07% algae DW), and the hot water fractions purest in fucoidans and alginates with a lower mannuronic-to-guluronic (M/G) ratio (2.91). Conversely, when beginning extraction procedures with 96% ethanol, the recovered yields of phlorotannins increased (0.43 g PGE/100 g algae DW), but there was a concomitant seven-fold decrease in the recovery of fucoxanthin in the subsequent 70% ethanol extract. This approach also led to less pure hot water fractions containing fucoidans, laminarans and alginates with a higher M/G ratio (5.50). Overall, this work unveiled the potential of the first extraction steps in sustainable and holistic cascade strategies to modulate the composition of food-grade extracts, creating prospects of their application as tailored functional ingredients in food products.
The Characterization of Subcutaneous Adipose Tissue in Sunit Sheep at Different Growth Stages: A Comprehensive Analysis of the Morphology, Fatty Acid Profile, and Metabolite Profile
Authors: Han Yunfei , He Xige , Yun Yueying , Chen Lu , Huang Yajuan , Wu Qiong , Qin Xia , Wu Haiyan , Wu Jindi , Sha Rina , Borjigin Gerelt
Published: February 2024

Adipose tissue is a crucial economically significant trait that significantly influences the meat quality and growth performance of domestic animals. To reveal the changes in adipose tissue metabolism during the growth of naturally grazing sheep, we evaluated the thickness, adipocyte morphology, fatty acid profile, and metabolite profile of subcutaneous adipose tissue (SAT) from naturally grazing Sunit sheep at 6, 18, and 30 months of age (referred to as Mth-6, Mth-18, and Mth-30, respectively). The fat thickness and adipocyte number were significantly increased with the growth of the sheep (p < 0.05), and the increase of which from Mth-18 to Mth-30 was less than that from Mth-6 to Mth-18. Additionally, the alpha-linolenic acid metabolism was enhanced and fatty acid (FA) elongation increased with growth. The metabolomic analysis revealed 76 differentially expressed metabolites (DEMs) in the SAT in different growth stages. Interestingly, we observed elongation of FAs in lipids correlated with sheep growth. Furthermore, the expression of acylcarnitines was downregulated, and fatty acid amides, aspartic acid, acetic acid and phosphocholine were upregulated in Mth-18 and Mth-30 compared to Mth-6. Altogether, the study found that the difference in SAT in Mth-6 was great compared to Mth-18 and Mth-30. An increase in fat deposition via adipocyte proliferation with the growth of the sheep in naturally grazing. The DEMs of acylcarnitines, fatty acid amides, aspartic acid, acetic acid, and phosphocholine emerged as potential key regulators of adipose tissue metabolism. These findings illustrate the variation in and metabolic mechanism of sheep adipose tissue development under natural grazing, thus providing valuable insights into improving the edible quality of sheep meat and developing the mutton sheep industry.
Origin and Clinical Impact of Early Multidrug-Resistant (MDR) Contamination in Patients Undergoing Pancreaticoduodenectomy
Authors: Sorrentino Martina , Capretti Giovanni , Nappo Gennaro , Gavazzi Francesca , Ridolfi Cristina , Pagnanelli Michele , Nebbia Martina , Morelli Paola , Zerbi Alessandro
Published: February 2024

Introduction: Infections caused by multidrug-resistant bacteria (MDR) occur more frequently after pancreaticoduodenectomy (PD) compared to other abdominal surgeries, and infective complications represent a major determinant of postoperative morbidity following PD. Preoperative biliary stent (PBS) placement often leads to biliary contamination, which plays a significant role in postoperative infections. The aim of this study is to evaluate the impact of MDR contamination on short-term postoperative outcomes in patients undergoing PD and to evaluate the relationship between MDR bacteria, PBS, and bile contamination. Methods: This is a retrospective study based on a prospectively maintained database including 825 consecutive patients who underwent pancreaticoduodenectomies (PDs). All procedures were performed by experienced pancreatic surgeons at a high-volume center and the patients were managed according to the same perioperative enhanced recovery protocol. Results: MDR bacteria were present in 17.5% of bile cultures, exclusively within the stented group. At the multivariate analysis, the development of major postoperative complications (MPC) was correlated with the presence of MDR bacteria in the bile (OR 1.66, 95% CI: 1.1–2.52; p = 0.02). MDR bacteria were detected early in the surgical drainage in 144 out of 825 patients (12.1%), with 72.2% having a previous biliary stent placement and 27.8% without stents (p < 0.001). Moreover, the development of an MPC was associated with the presence of MDR bacteria in the drainage (OR = 1.81, 95% CI: 1.21–2.73, p = 0.0042). Conclusions: We demonstrated that MDR contamination worsens the short-term outcomes of patients undergoing PDs. Specifically, when MDR bacteria are present in both the bile and drainage, there is a statistically significant increase in the incidence of major postoperative complications (MPC). Our data suggest that the majority of MDR surgical site infections stem from biliary contamination resulting from the placement of a preoperative biliary stent (PBS).
Firefighter Health: A Narrative Review of Occupational Threats and Countermeasures
Authors: Gonzalez Drew E. , Lanham Sarah N. , Martin Steven E. , Cleveland Richard E. , Wilson Thad E. , Langford Emily L. , Abel Mark G.
Published: February 2024

Structural firefighters are responsible for protecting properties and saving lives during emergency operations. Despite efforts to prepare firefighters for these hazardous occupational demands, the unfortunate reality is that the incidence of health morbidities is increasing within the fire service. Specifically, cardiovascular disease, cancer, and mental health disorders are among the most documented morbidities in firefighters. Pubmed and Google Scholar search engines were used to identify peer-reviewed English language manuscripts that evaluated firefighters’ occupational health threats, allostatic factors associated with their occurrence, and evidence-based strategies to mitigate their impact. This narrative review provides fire departments, practitioners, and researchers with evidence-based practices to enhance firefighters’ health.
Cross-Cultural Adaptation and Validation of the Arabic Version of the Prolapse Quality of Life Questionnaire in the United Arab Emirates
Authors: Alzarooni Asma Abdelrahman , Shousha Tamer Mohamed , Kim Meeyoung
Published: February 2024

Background: Given the extensive translation of the Prolapse Quality of Life Questionnaire (P-QoL) into many languages, it is imperative to develop an Arabic version to facilitate the study of pelvic organ health within the Arabian culture. Objective: The aim of this study was to investigate, cross-culturally adapt, and validate the Arabic version of the P-QoL. Study Design: This study involved cross-cultural adaptation and psychometric testing. Methods: A total of 90 participants were included in the study. This cross-sectional study was carried out in two phases; during phase I, the P-QoL was translated and adapted from English into Arabic. The Arabic version was psychometrically validated during phase II using the test–retest reliability and internal consistency with Cronbach’s alpha coefficient, convergent construct (CC) validity among the four study tools using Spearman’s coefficient (r), and discriminative validity using Mann–Whitney test to find the differences between the means of the two samples. Results: A satisfactory level of semantic, conceptual, idiomatic, and content comparability was reached in the cross-cultural adaptation of the Arabic version of the P-QoL. The internal consistency was high in terms of psychometric validation, with a Cronbach’s alpha coefficient of 0.971 for the P-QoL. The test–retest results showed high reliability, with the interclass correlation coefficient (ICC) of the P-QoL determined as 0.987. The convergent construct validity was highly acceptable (moderately strong), reflecting a positive correlation between the Arabic version of the P-QoL and the Australian Pelvic Floor Dysfunction Questionnaire (APFD) (r = 0.68; p < 0.001). Similarly, a significant convergent validity of the Arabic version of the P-QoL and the Visual Analogue Scale (VAS) (r = 0.47; p < 0.001) was observed, as well as a correlation between the APFD and the VAS (r = 0.46; p < 0.001). However, there was no significant correlation between the 12-Item Short-Form Survey (SF-12), the P-QoL, the APFD, and the VAS. Conclusion: Based on the significant correlation found between the Arabic APFD and the VAS, the results reveal good reliability, internal consistency, and construct validity. It is recommended that Arabic-speaking females with pelvic organ prolapse use the Arabic version of the P-QoL. More research is needed to assess the responsiveness of the P-QoL.
On the Effect of Vibrotactile Stimulation in Essential Tremor
Authors: Cabral Ariana Moura , Lora-Millán Julio Salvador , Pereira Adriano Alves , Rocon Eduardo , Andrade Adriano de Oliveira
Published: February 2024

(1) Background: Vibrotactile stimulation has been studied for tremor, but there is little evidence for Essential Tremor (ET). (2) Methods: This research employed a dataset from a previous study, with data collected from 18 individuals subjected to four vibratory stimuli. To characterise tremor changes before, during, and after stimuli, time and frequency domain features were estimated from the signals. Correlation and regression analyses verified the relationship between features and clinical tremor scores. (3) Results: Individuals responded differently to vibrotactile stimulation. The 250 Hz stimulus was the only one that reduced tremor amplitude after stimulation. Compared to the baseline, the 250 Hz and random frequency stimulation reduced tremor peak power. The clinical scores and amplitude-based features were highly correlated, yielding accurate regression models (mean squared error of 0.09). (4) Conclusions: The stimulation frequency of 250 Hz has the greatest potential to reduce tremors in ET. The accurate regression model and high correlation between estimated features and clinical scales suggest that prediction models can automatically evaluate and control stimulus-induced tremor. A limitation of this research is the relatively reduced sample size.
Adherence to the Mediterranean Diet in Children and Adolescents and Association with Multiple Outcomes: An Umbrella Review
Authors: Masini Alice , Dallolio Laura , Sanmarchi Francesco , Lovecchio Francesco , Falato Mario , Longobucco Yari , Lanari Marcello , Sacchetti Rossella
Published: February 2024

The Mediterranean Diet (MD) has been linked to numerous health benefits. This umbrella review aims to synthesize evidence from systematic reviews on the MD’s impact on children and adolescents aged 6 to 19 years. Following Joanna Briggs Institute guidelines, we included 11 systematic reviews focusing on the MD’s effects on health outcomes, such as asthma, anthropometric measures, physical fitness, and inflammatory markers. The results indicate a protective role of the MD against childhood asthma due to its antioxidant-rich components. However, evidence for its impact on allergic conditions like rhinitis and eczema is inconclusive. Findings regarding anthropometric outcomes, particularly BMI, are limited and inconsistent. A positive correlation was observed between MD adherence and increased physical activity, improved physical fitness, quality of life, and reduced sedentary behavior. Additionally, the MD showed an inverse relationship with pro-inflammatory biomarkers, suggesting anti-inflammatory benefits. The inconsistency in BMI findings and the potential anti-inflammatory properties of the MD warrant further longitudinal research. Future studies should explore the MD’s impact on cognitive functions, academic performance, and mental health in this age group, highlighting the significance of establishing healthy eating habits early in life.
A Fresh Perspective on Examining Population Emotional Well-Being Trends by Internet Search Engine: An Emerging Composite Anxiety and Depression Index
Authors: Wang Yu , Deng Heming , Gao Sunan , Li Tongxu , Wang Feifei
Published: February 2024

Traditional assessments of anxiety and depression face challenges and difficulties when it comes to understanding trends in-group psychological characteristics. As people become more accustomed to expressing their opinions online, location-based online media and cutting-edge algorithms offer new opportunities to identify associations between group sentiment and economic- or healthcare-related variables. Our research provides a novel approach to analyzing emotional well-being trends in a population by focusing on retrieving online information. We used emotionally enriched texts on social media to build the Public Opinion Dictionary (POD). Then, combining POD with the word vector model and search trend, we developed the Composite Anxiety and Depression Index (CADI), which can reflect the mental health level of a region during a specific time period. We utilized the representative external data by CHARLS to validate the effectiveness of CADI, indicating that CADI can serve as a representative indicator of the prevalence of mental disorders. Regression and subgroup analysis are employed to further elucidate the association between public mental health (measured by CADI) with economic development and medical burden. The results of comprehensive regression analysis show that the Import–Export index (−16.272, p < 0.001) and average cost of patients (4.412, p < 0.001) were significantly negatively associated with the CADI, and the sub-models stratificated by GDP showed the same situation. Disposable income (−28.389, p < 0.001) became significant in the subgroup with lower GDP, while the rate of unemployment (2.399, p < 0.001) became significant in the higher subgroup. Our findings suggest that an unfavorable economic development or unbearable medical burden will increase the negative mental health of the public, which was consistent across both the full and subgroup models.
Unearthing Inequities in the Relationship between Multiple Sociodemographic Factors and Diverse Elements of Park Availability and Quality in a Major Southern Metropolitan Region
Authors: Hallum Shirelle H. , Wende Marilyn E. , Hesam Shariati Farnaz , Thomas Kelsey M. , Chupak Anna L. , Witherspoon Eleanor , Kaczynski Andrew T.
Published: February 2024

Parks are critical components of healthy communities. This study explored neighborhood socioeconomic and racial/ethnic inequalities in park access and quality in a large U.S. southeastern metropolitan region. A total of 241 block groups were examined, including 77 parks. For each block group, we obtained multiple sociodemographic indicators, including unemployment rate, education level, renter-occupied housing, poverty rate, and racial/ethnic minority composition. All parks were mapped using geographical information systems and audited via the Community Park Audit Tool to evaluate their features and quality. We analyzed seven diverse elements of park quality (transportation access, facility availability, facility quality, amenity availability, park aesthetics, park quality concerns, and neighborhood quality concerns), as well as an overall park quality score by calculating the mean for all parks within each block group. The mean percent of residents below 125% of the poverty level and the percentage of renter-occupied housing units were significantly higher among block groups with any parks in comparison to block groups with no parks. In addition, there were significant positive associations between park transportation access scores and both the percentage of residents with less than high school education and the percent identifying as non-Hispanic white. Moreover, there was a significant negative association between park amenity availability and the block group’s unemployed population. Further, a significant negative association between park aesthetics and the population with a lower than high school education percentage was observed. Revealed differences in park availability, park acreage, and park quality dimensions emphasized the need for targeted policy, programmatic, and infrastructure interventions to improve park access and quality and address health disparities.
Benzene Exposure and Lung Cancer Risk: A Systematic Review and Meta-Analysis of Human Studies
Authors: Chiavarini Manuela , Rosignoli Patrizia , Sorbara Beatrice , Giacchetta Irene , Fabiani Roberto
Published: February 2024

Lung cancer is a leading cause of death with nearly 1.8 million deaths estimated worldwide in 2020. Although benzene is classified as a human carcinogen (Group 1) on the basis of its association with acute myeloid/non-lymphocytic leukaemia, there is still limited evidence that it may influence lung cancer risk. This study examined the potential link between benzene exposure and risk of lung cancer using a systematic review of epidemiological studies and meta-analysis. We searched through PubMed, Web of Science and Scopus databases up to 10 February 2023 to identify all articles on the association between benzene exposure and lung cancer (incidence or prevalence) and/or mortality. We extracted the risk estimates of the highest and the lowest reported categories of benzene exposure and conducted a meta-analysis using a random-effects model. Heterogeneity and publication bias were analysed using an I2 test and funnel plots asymmetry, respectively. Twenty-one studies were included in the final analysis, with a total of 10,750 lung cancer cases and 2899 lung cancer deaths. Overall, risk estimates of lung cancer prevalence and mortality in association with benzene exposure were 1.20 (n = 14; 95% CI 1.05–1.37) and 1.15 (n = 13; 95% CI 1.02–1.30), respectively. In all cases, heterogeneity was quite large, while no significant publication bias was observed. When only studies that adjusted for smoking habit were selected, the risk for lung cancer increased by up to 34% (n = 9; 95% CI 1.10–1.64). Our data, which show a strong association between benzene exposure and lung cancer risk, may have important public health implications. However, further studies are needed to identify the lung cancer risk associated with benzene exposure considering different smoking conditions.
Bacteria: Potential Make-or-Break Determinants of Celiac Disease
Authors: Roque Ana , Pereira Sónia Gonçalves
Published: February 2024

Celiac disease is an autoimmune disease triggered by dietary gluten in genetically susceptible individuals that primarily affects the small intestinal mucosa. The sole treatment is a gluten-free diet that places a social and economic burden on patients and fails, in some, to lead to symptomatic or mucosal healing. Thus, an alternative treatment has long been sought after. Clinical studies on celiac disease have shown an association between the presence of certain microbes and disease outcomes. However, the mechanisms that underlie the effects of microbes in celiac disease remain unclear. Recent studies have employed disease models that have provided insights into disease mechanisms possibly mediated by bacteria in celiac disease. Here, we have reviewed the bacteria and related mechanisms identified so far that might protect from or incite the development of celiac disease. Evidence indicates bacteria play a role in celiac disease and it is worth continuing to explore this, particularly since few studies, to the best of our knowledge, have focused on establishing a mechanistic link between bacteria and celiac disease. Uncovering host–microbe interactions and their influence on host responses to gluten may enable the discovery of pathogenic targets and development of new therapeutic or preventive approaches.
A Novel Drosophila Model of Alzheimer’s Disease to Study Aβ Proteotoxicity in the Digestive Tract
Authors: Elovsson Greta , Klingstedt Therése , Brown Mikaela , Nilsson K. Peter R. , Brorsson Ann-Christin
Published: February 2024

Amyloid-β (Aβ) proteotoxicity is associated with Alzheimer’s disease (AD) and is caused by protein aggregation, resulting in neuronal damage in the brain. In the search for novel treatments, Drosophila melanogaster has been extensively used to screen for anti-Aβ proteotoxic agents in studies where toxic Aβ peptides are expressed in the fly brain. Since drug molecules often are administered orally there is a risk that they fail to reach the brain, due to their inability to cross the brain barrier. To circumvent this problem, we have designed a novel Drosophila model that expresses the Aβ peptides in the digestive tract. In addition, a built-in apoptotic sensor provides a fluorescent signal from the green fluorescent protein as a response to caspase activity. We found that expressing different variants of Aβ1–42 resulted in proteotoxic phenotypes such as reduced longevity, aggregate deposition, and the presence of apoptotic cells. Taken together, this gut-based Aβ-expressing fly model can be used to study the mechanisms behind Aβ proteotoxicity and to identify different substances that can modify Aβ proteotoxicity.
Antiseptic, Hemostatic, and Wound Activity of Poly(vinylpyrrolidone)-Iodine Gel with Trimethyl Chitosan
Authors: Padalhin Andrew , Ryu Hyun Seok , Yoo Seung Hyeon , Abueva Celine , Seo Hwee Hyon , Park So Young , Chung Phil-Sang , Woo Seung Hoon
Published: February 2024

Wound management practices have made significant advancements, yet the search for improved antiseptics persists. In our pursuit of solutions that not only prevent infections but also address broader aspects of wound care, we investigated the impact of integrating trimethyl chitosan (TMC) into a widely used poly(vinylpyrrolidone)-iodine gel (PVP-I gel). Our study assessed the antimicrobial efficacy of the PVP gel with TMC against Escherichia coli, Staphylococcus aureus, multidrug-resistant S. aureus MRSA, and Candida albicans. Additionally, we compared hemostatic effects using a liver puncture bleeding model and evaluated wound healing through histological sections from full-thickness dermal wounds in rats. The results indicate that incorporating TMC into the commercially available PVP-I gel did not compromise its antimicrobial activity. The incorporation of TMC into the PVP-I gel markedly improves its hemostatic activity. The regular application of the PVP-I gel with TMC resulted in an increased blood vessel count in the wound bed and facilitated the development of thicker fibrous tissue with a regenerated epidermal layer. These findings suggest that TMC contributes not only to antimicrobial activity but also to the intricate processes of tissue regeneration. In conclusion, incorporating TMC proves beneficial, making it a valuable additive to commercially available antiseptic agents.
The Potential Use of Vitamin D3 and Phytochemicals for Their Anti-Ageing Effects
Authors: Santa Kazuki , Kumazawa Yoshio , Watanabe Kenji , Nagaoka Isao
Published: February 2024

Unlike other vitamins, vitamin D3 is synthesised in skin cells in the body. Vitamin D3 has been known as a bone-related hormone. Recently, however, it has been considered as an immune vitamin. Vitamin D3 deficiency influences the onset of a variety of diseases. Vitamin D3 regulates the production of proinflammatory cytokines such as tumour necrosis factor-α (TNF-α) through binding to vitamin D receptors (VDRs) in immune cells. Since blood levels of vitamin D3 (25-OH-D3) were low in coronavirus disease 2019 (COVID-19) patients, there has been growing interest in the importance of vitamin D3 to maintaining a healthy condition. On the other hand, phytochemicals are compounds derived from plants with over 7000 varieties and have various biological activities. They mainly have health-promoting effects and are classified as terpenoids, carotenoids, flavonoids, etc. Flavonoids are known as the anti-inflammatory compounds that control TNF-α production. Chronic inflammation is induced by the continuous production of TNF-α and is the fundamental cause of diseases like obesity, dyslipidaemia, diabetes, heart and brain diseases, autoimmune diseases, Alzheimer’s disease, and cancer. In addition, the ageing process is induced by chronic inflammation. This review explains the cooperative effects of vitamin D3 and phytochemicals in the suppression of inflammatory responses, how it balances the natural immune response, and its link to anti-ageing effects. In addition, vitamin D3 and phytochemicals synergistically contribute to anti-ageing by working with ageing-related genes. Furthermore, prevention of ageing processes induced by the chronic inflammation requires the maintenance of healthy gut microbiota, which is related to daily dietary habits. In this regard, supplementation of vitamin D3 and phytochemicals plays an important role. Recently, the association of the prevention of the non-disease condition called “ME-BYO” with the maintenance of a healthy condition has been an attractive regimen, and the anti-ageing effect discussed here is important for a healthy and long life.
Exploring the Role of Surface and Mitochondrial ATP-Sensitive Potassium Channels in Cancer: From Cellular Functions to Therapeutic Potentials
Authors: Moon Dong-Oh
Published: February 2024

ATP-sensitive potassium (KATP) channels are found in plasma membranes and mitochondria. These channels are a type of ion channel that is regulated by the intracellular concentration of adenosine triphosphate (ATP) and other nucleotides. In cell membranes, they play a crucial role in linking metabolic activity to electrical activity, especially in tissues like the heart and pancreas. In mitochondria, KATP channels are involved in protecting cells against ischemic damage and regulating mitochondrial function. This review delves into the role of KATP channels in cancer biology, underscoring their critical function. Notably responsive to changes in cellular metabolism, KATP channels link metabolic states to electrical activity, a feature that becomes particularly significant in cancer cells. These cells, characterized by uncontrolled growth, necessitate unique metabolic and signaling pathways, differing fundamentally from normal cells. Our review explores the intricate roles of KATP channels in influencing the metabolic and ionic balance within cancerous cells, detailing their structural and operational mechanisms. We highlight the channels’ impact on cancer cell survival, proliferation, and the potential of KATP channels as therapeutic targets in oncology. This includes the challenges in targeting these channels due to their widespread presence in various tissues and the need for personalized treatment strategies. By integrating molecular biology, physiology, and pharmacology perspectives, the review aims to enhance the understanding of cancer as a complex metabolic disease and to open new research and treatment avenues by focusing on KATP channels. This comprehensive approach provides valuable insights into the potential of KATP channels in developing innovative cancer treatments.
Structural Characterization and In Vitro Anti-Inflammatory Activity of Polysaccharides Isolated from the Fruits of Rosa laevigata
Authors: Peng Song , Gu Pengfei , Mao Ningning , Yu Lin , Zhu Tianyu , He Jin , Yang Yang , Liu Zhenguang , Wang Deyun
Published: February 2024

RLPa-2 (Mw 15.6 kDa) is a polysaccharide isolated from Rosa laevigata Michx. It consists of arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), xylose (Xyl), and galacturonic acid (Gal-UA) with a molar ratio of 1.00:0.91:0.39:0.34:0.25:0.20. Structural characterization was performed by methylation and NMR analysis, which indicated that RLPa-2 might comprise →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, α-L-Araf-(1→, →2,4)-α-D-Glcp-(1→, β-D-Xylp, and α-L-Rhap. In addition, the bioactivity of RLPa-2 was assessed through an in vitro macrophage polarization assay. Compared to positive controls, there was a significant decrease in the expression of M1 macrophage markers (CD80, CD86) and p-STAT3/STAT3 protein. Additionally, there was a down-regulation in the production of pro-inflammatory mediators (NO, IL-6, TNF-α), indicating that M1 macrophage polarization induced with lipopolysaccharide (LPS) and interferon-γ (IFN-γ) stimulation could be inhibited by RLPa-2. These findings demonstrate that the RLPa-2 might be considered as a potential anti-inflammatory drug to reduce inflammation.
Theoretical Study on the Structures and Stabilities of CunZn3O3 (n = 1–4) Clusters: Sequential Doping of Zn3O3 Cluster with Cu Atoms
Authors: Tao Zhi-Wei , Zou Han-Yi , Li Hong-Hui , Wang Bin , Chen Wen-Jie
Published: February 2024

Density functional theory (DFT) and coupled cluster theory (CCSD(T)) calculations are performed to investigate the geometric and electronic structures and chemical bonding of a series of Cu-doped zinc oxide clusters: CunZn3O3 (n = 1–4). The structural evolution of CunZn3O3 (n = 1–4) clusters may reveal the aggregation behavior of Cu atoms on the Zn3O3 cluster. The planar seven-membered ring of the CuZn3O3 cluster plays an important role in the structural evolution; that is, the Cu atom, Cu dimer (Cu2) and Cu trimer (Cu3) anchor on the CuZn3O3 cluster. Additionally, it is found that CunZn3O3 clusters become more stable as the Cu content (n) increases. Bader charge analysis points out that with the doping of Cu atoms, the reducibility of Cu aggregation (Cun−1) on the CuZn3O3 cluster increases. Combined with the d-band centers and the surface electrostatic potential (ESP), the reactivity and the possible reaction sites of CunZn3O3 (n = 1–4) clusters are also illustrated.
Pierid Butterflies, Legume Hostplants, and Parasitoids in Urban Areas of Southern Florida
Authors: Koptur Suzanne , Primoli Andrea , Paulino-Neto Hipólito , Whitfield James
Published: February 2024

Are parasitoids less likely to find their Lepidoptera hosts on non-native hostplants than native hostplants? We predicted that with longer periods of coevolution between herbivores and the plants they consume, the parasitoids that provide top-down control would be more attuned to finding their hosts on native plants. To test this hypothesis, we collected immature stages of sulfur butterflies (the cloudless sulfur (Phoebis sennae) and the orange-barred sulfur (Phoebis agarithe) over a three-year period (2008–2011) from native and ornamental hostplants in the genus Senna in three different parts of the urban landscape of Miami, Florida, USA. We reared the immature specimens to pupation and either eclosion of adults or emergence of parasitoids and compared the levels of parasitization among the three areas, and among native vs. exotic hostplants. We found, contrary to our prediction, that caterpillars feeding on non-native leguminous hostplant species were more likely to be parasitized than those feeding on native hostplants. We discuss this surprising finding in the light of recent findings in other plant/herbivore/parasitoid systems.
A Systematic Method for Assessing the Machine Performance of Material Extrusion Printers
Authors: Spitaels Laurent , Nieto Fuentes Endika , Rivière-Lorphèvre Edouard , Arrazola Pedro-José , Ducobu François
Published: February 2024

The performance assessment of additive manufacturing (AM) printers is still a challenge since no dedicated standard exists. This paper proposes a systematic method for evaluating the dimensional and geometrical performance of such machines using the concept of machine performance. The method was applied to an Ultimaker 2+ printer producing parts with polylactic acid (PLA). The X and Y axes of the printer were the most performant and led to narrower potential and real tolerance intervals than the Z axis. The proposed systematic framework can be used to assess the performance of any material extrusion printer and its achievable tolerance intervals.
Advanced Underwater Measurement System for ROVs: Integrating Sonar and Stereo Vision for Enhanced Subsea Infrastructure Maintenance
Authors: Zhang Jiawei , Han Fenglei , Han Duanfeng , Yang Jianfeng , Zhao Wangyuan , Li Hansheng
Published: February 2024

In the realm of ocean engineering and maintenance of subsea structures, accurate underwater distance quantification plays a crucial role. However, the precision of such measurements is often compromised in underwater environments due to backward scattering and feature degradation, adversely affecting the accuracy of visual techniques. Addressing this challenge, our study introduces a groundbreaking method for underwater object measurement, innovatively combining image sonar with stereo vision. This approach aims to supplement the gaps in underwater visual feature detection with sonar data while leveraging the distance information from sonar for enhanced visual matching. Our methodology seamlessly integrates sonar data into the Semi-Global Block Matching (SGBM) algorithm used in stereo vision. This integration involves introducing a novel sonar-based cost term and refining the cost aggregation process, thereby both elevating the precision in depth estimations and enriching the texture details within the depth maps. This represents a substantial enhancement over existing methodologies, particularly in the texture augmentation of depth maps tailored for subaquatic environments. Through extensive comparative analyses, our approach demonstrates a substantial reduction in measurement errors by 1.6%, showing significant promise in challenging underwater scenarios. The adaptability and accuracy of our algorithm in generating detailed depth maps make it particularly relevant for underwater infrastructure maintenance, exploration, and inspection.
Discrimination of Internal Solitary Waves from Coarse Time Resolution Field Observational Data by Using Wavelet Analysis
Authors: Xu Jiexin , Chen Shaomin , Gong Yankun , Chen Zhiwu , Cai Shuqun , Li Daning
Published: February 2024

Internal solitary waves (ISWs) are large-amplitude internal waves which would destroy underwater engineering. Finding an easy way to discriminate ISWs from field observational data is crucial. Two time--series datasets, one contained ISWs and another only containing internal tides, were obtained from filed observations. Based on single-layer velocity data, wavelet spectrum shows significant high value in short time-scale domain when ISWs pass, whilst having no signal in that domain when only internal tides exist, indicating the capability of wavelet analysis on ISWs detection. Wavelet variances of the dataset with ISWs has a bimodal distribution versus periods with two peaks around 40 min and 110 min, which can also be reproduced by a numerical model, indicating that the energy within period band of 10–120 min is caused by ISWs. By using the conceived signal processing techne, data reconstruction can precisely obtain the arrival time of ISWs and retain about 91.4% of the original signal. It is found that, based on a field observational dataset with even a coarse sampling interval for up to 20 min, the existence of ISWs can be easily discriminated by using wavelet analysis, which provides us an economic method for the early warning of ISWs in ocean engineering.
Soft-Shell Production of the Invasive Atlantic Blue Crab Callinectes sapidus in the Lesina Lagoon (SE Italy): A First Assessment
Authors: Cilenti Lucrezia , Lago Nicola , Lillo Antonio Oscar , Li Veli Daniel , Scirocco Tommaso , Mancinelli Giorgio
Published: February 2024

The current invasion of the Mediterranean Sea by the Atlantic blue crab Callinectes sapidus requires the implementation of effective strategies of control and management. In native areas, the species is highly appreciated as a hard- and soft-shell seafood, and hard-shell fisheries are developing in a number of invaded countries. Here, to verify alternative approaches for enhancing the commercial value of the species, we carried out a pilot experiment to test a flow-through system for the production of soft-shell blue crabs. Fifty crabs were collected in the Lesina lagoon (Adriatic Sea, Italy) and inspected for the coloration of the line on the distal edge of the fifth pereiopod. Accordingly, they were grouped into three groups showing no, white, and red lines, symptomatic of the progression of the pre-molt phase, and maintained for 31 days in a flow-through pond system. The overall mortality rate determined during the trial was similar across the three groups and ranged between 10 and 20%, indicating that the flow-through system was effective for rearing the crabs. In addition, the molting rate was 10%, 65%, and 85% for individuals at an early, intermediate, and advanced pre-molt stage, confirming that the line coloration can be used as an effective indicator of the advancement of the molting process. Our study provides the first contribution towards the introduction in the Mediterranean fishery sector of novel procedures and practices for the production of soft-shell blue crabs, which may represent a cost-effective strategy to enhance the capture and commercialization of this invasive species as a high-quality and valuable shellfish product.