bluuebunny/crossref_metadata_2025 huggingface dataset:
Multidimensional voiceprint feature assessment system for identifying the depression in children and adolescents: a diagnostic test
Authors: Luo Jie , Liu Mengqi , Feng Lihui , Li Zhaojun , Wu Yuanzhen , Lu Jihua , He Fan
Published: May 2023

Objective

We designed a diagnostic test to evaluate the effectiveness and accuracy of a multidimensional voiceprint feature diagnostic assessment (MVFDA) system vs. the 24-item Hamilton Rating Scale for Depression (HAMD-24) for adjunctive diagnosis of children and adolescents with major depressive disorder (MDD).

Methods

This study included 55 children aged 6–16 years who were clinically diagnosed with MDD according to the DSM-5 and analyzed by professional physicians, and 55 healthy children (typically developing). Each subject completed a voice recording and was scored on the HAMD-24 scale by a trained rater. We calculated the validity indices, including sensitivity, specificity, Youden's index, likelihood ratio, and other indices including predictive value, diagnostic odds ratio, diagnostic accuracy, and area under the curve (AUC), to assess the effectiveness of the MVFDA system in addition to the HAMD-24.

Results

The sensitivity (92.73 vs. 76.36%) and the specificity (90.91 vs. 85.45%) of the MVFDA system are significantly higher than those of the HAMD-24. The AUC of the MVFDA system is also higher than that of the HAMD-24. There is a statistically significant difference between the groups (p < 0.05), and both of them have high diagnostic accuracy. In addition, the diagnostic efficacy of the MVFDA system is higher than that of HAMD-24 in terms of the Youden index, diagnostic accuracy, likelihood ratio, diagnostic odds ratio, and predictive value.

Conclusion

The MVFDA has performed well in clinical diagnostic trials for the identification of MDD in children and adolescents by capturing objective sound features. Compared with the scale assessment method, the MVFDA system could be further promoted in clinical practice due to its advantages of simple operation, objective rating, and high diagnostic efficiency.
Hippocampal BAIAP2 prevents chronic mild stress-induced depression-like behaviors in mice
Authors: Fu Yaling , Guo Xiangfei , Yang Rui , Feng Hao , Yin Xueyong , Wang Shuang , Song Li , Wang Xi , Zhao Penghui , Wang Sheng , Shi Yun , Shi Haishui
Published: May 2023

Background

The pathogenesis of depression is closely related to changes in hippocampal synaptic plasticity; however, the underlying mechanism is still unclear. Brain-specific angiogenesis inhibitor 1-associated protein 2 (BAIAP2), a postsynaptic scaffold protein in excitatory synapses important for synaptic plasticity, is highly expressed in the hippocampus and has been implicated in several psychiatric disorders. However, the role of BAIAP2 in depression remains poorly understood.

Methods

In the present study, a mouse model of depression was established via exposure to chronic mild stress (CMS). An adeno-associated virus (AAV) vector expressing BAIAP2 was injected into the hippocampal brain region of mice and a BAIAP2 overexpression plasmid was transfected into HT22 cells to upregulate BAIAP2 expression. Depression- and anxiety-like behaviors and dendritic spine density were examined in mice using behavioral tests and Golgi staining, respectively. In vitro, hippocampal HT22 cells were treated with corticosterone (CORT) to simulate the stress state, and the effect of BAIAP2 on CORT-induced cell injury was explored. Reverse transcription-quantitative PCR and western blotting were employed to determine the expression levels of BAIAP2 and those of the synaptic plasticity-related proteins glutamate receptor ionotropic, AMPA 1 (GluA1), and synapsin 1 (SYN1).

Results

Mice exposed to CMS exhibited depression- and anxiety-like behaviors accompanied by decreased levels of BAIAP2 in the hippocampus. In vitro, the overexpression of BAIAP2 increased the survival rate of CORT-treated HT22 cells and upregulated the expression of GluA1 and SYN1. Consistent with the in vitro data, the AAV-mediated overexpression of BAIAP2 in the hippocampus of mice significantly inhibited CMS-induced depression-like behavior, concomitant with increases in dendritic spine density and the expression of GluA1 and SYN1 in hippocampal regions.

Conclusion

Our findings indicate that hippocampal BAIAP2 can prevent stress-induced depression-like behavior and may be a promising target for the treatment of depression or other stress-related diseases.
A virtual pilot optimization trial for African American/Black and Latino persons with non-suppressed HIV viral load grounded in motivational interviewing and behavioral economics
Authors: Filippone Prema , Serrano Samantha , Gwadz Marya , Cleland Charles M. , Freeman Robin , Linnemayr Sebastian , Cluesman Sabrina R. , Campos Stephanie , Rosmarin-DeStefano Corey , Amos Brianna , Israel Khadija
Published: May 2023

Introduction

Virtual and low-touch behavioral interventions are needed for African American/Black and Latino persons living with HIV (PLWH) with barriers to HIV viral suppression, particularly during COVID-19. Guided by the multiphase optimization strategy, we explored three components for PLWH without viral suppression, grounded in motivational interviewing and behavioral economics: (1) motivational interviewing counseling, (2) 21-weeks of automated text messages and quiz questions about HIV management, and (3) financial rewards for viral suppression (lottery prize vs. fixed compensation).

Methods

This pilot optimization trial used sequential explanatory mixed methods to explore the components' feasibility, acceptability, and preliminary evidence of effects using an efficient factorial design. The primary outcome was viral suppression. Participants engaged in baseline and two structured follow-up assessments over an 8-month period, and provided laboratory reports to document HIV viral load. A subset engaged in qualitative interviews. We carried out descriptive quantitative analyses. Then, qualitative data were analyzed using directed content analysis. Data integration used the joint display method.

Results

Participants (N = 80) were 49 years old, on average (SD = 9), and 75% were assigned male sex at birth. Most (79%) were African American/Black, and the remainder were Latino. Participants were diagnosed with HIV 20 years previously on average (SD = 9). Overall, components were feasible (>80% attended) and acceptability was satisfactory. A total of 39% (26/66) who provided laboratory reports at follow-up evidenced viral suppression. Findings suggested no components were entirely unsuccessful. The lottery prize compared to fixed compensation was the most promising component level. In qualitative analyses, all components were seen as beneficial to individual wellbeing. The lottery prize appeared more interesting and engaging than fixed compensation. However, structural barriers including financial hardship interfered with abilities to reach viral suppression. The integrated analyses yielded areas of convergence and discrepancy and qualitative findings added depth and context to the quantitative results.

Conclusions

The virtual and/or low-touch behavioral intervention components tested are acceptable and feasible and show enough potential to warrant refinement and testing in future research, particularly the lottery prize. Results must be interpreted in the context of the COVID-19 pandemic.

Trial registration

NCT04518241 (https://clinicaltrials.gov/ct2/show/NCT04518241).
Warm giant exoplanet characterisation: current state, challenges and outlook
Authors: Müller Simon , Helled Ravit
Published: May 2023

The characterisation of giant exoplanets is crucial to constrain giant planet formation and evolution theory and for putting the solar-system’s giant planets in perspective. Typically, mass-radius (M-R) measurements of moderately irradiated warm Jupiters are used to estimate the planetary bulk composition, which is an essential quantity for constraining giant planet formation, evolution and structure models. The successful launch of the James Webb Space Telescope (JWST) and the upcoming ARIEL mission open a new era in giant exoplanet characterisation as atmospheric measurements provide key information on the composition and internal structure of giant exoplanets. In this review, we discuss how giant planet evolution models are used to infer the planetary bulk composition, and the connection between the compositions of the interior and atmosphere. We identify the important theoretical uncertainties in evolution models including the equations of state, atmospheric models, chemical composition, interior structure and main energy transport processes. Nevertheless, we show that atmospheric measurements by JWST and ARIEL and the accurate determination of stellar ages by PLATO can significantly reduce the degeneracy in the inferred bulk composition. Furthermore, we discuss the importance of evolution models for the characterisation of direct-imaged planets. We conclude that giant planet theory has a critical role in the interpretation of observation and emphasise the importance of advancing giant planet theory.
Perspectives on pharmacologic strategies in the management of meningoencephalomyelitis of unknown origin in dogs
Authors: Beasley Michaela J. , Shores Andy
Published: May 2023

There are many non-infectious inflammatory diseases, assumed to be immune-mediated in origin, recognized to affect the nervous system in canine patients. Concentrating on meningoencephalomyelitis of unknown origin, we will discuss the medications used to treat the underlying disease process, focusing on their adverse effects, therapeutic monitoring when necessary and effectiveness. The literature overwhelmingly supports the use of a steroid/ Cytosar® or steroid/ cyclosporine treatment protocol with the steroid tapered after the acute phase of the disease, leaving the secondary medication to control the disease long term. The decision on when and how quickly to taper the steroid is clinician dependent as a best practices has not been established in the literature. Also discussed will be the supportive care treatments often needed in the acute phase of these patients’ diagnosis and treatment such as anti-edema and anti-epileptic agents.
A case report of recurrent leiomyosarcoma with chondrosarcoma differentiation in the abdominal wall and a review of the literature
Authors: Zuo Xuan , Wu Wei L. , Shi Peng , Liu Tian M. , Yu Na , Li Lei
Published: May 2023

Leiomyosarcoma with heterologous differentiation is relatively rare. To date, only 19 cases have been reported in the English literature. Heterologous components frequently show histological pleomorphism, while those exhibiting well-differentiated morphology are seldom reported. Here, we report a 34-year-old female diagnosed with leiomyosarcoma and developed abdominal wall recurrence 8 years after primary surgery. The recurrent tumor mainly comprised well-differentiated chondrosarcoma except a single focus of leiomyosarcoma. Due to the rarity and prolonged onset of such a transition, our case provides insight into the understanding of this phenomenon.
Análisis de la gestión del talento humano en el contexto empresarial actual: una revisión bibliográfica
Authors: Ramírez-Torres William Eduardo
Published: May 2023

Esta investigación analiza la capacidad competitiva del talento humano en el contexto empresarial actual, mediante aplicación de modelos organizacionales y por competencias laborales en las áreas de gestión humana. Para ello, se realizó una revisión de literatura, en las bases de datos bibliográficas Scopus y Web of Science, en el periodo 2002-2022; utilizando para el proceso de refinación de la búsqueda la metodología PRISMA. Se incluyen en el análisis aquellos estudios, en los que se identifica la incorporación de modelos para la formación de competencias corporativas, técnicas y tecnológicas; la innovación, orientación al logro, aprendizaje organizacional, liderazgo, responsabilidad social y la integración sistémica entre lo operativo y estratégico. Dentro de las conclusiones, se resalta que la competitividad de las organizaciones se potencia cuando éstas integran la innovación en los procesos de cualificación del personal, la creación de nuevas formas de laborar y se actualizan los estilos de gerencia organizacional.
The Impact of COVID-19 on Curriculum and Employability in Lebanon
Authors: Boustani Nada Mallah
Published: May 2023

The COVID-19 pandemic negatively impacting the whole service sector, including higher education, has forced universities to quickly innovate and recreate. The sanitary crisis resulted in the greatest disruption to educational systems in human history, as well as a shift in the nature, qualifications, and mix of the workforce. The reopening of higher education institutions is another concern, with numerous new operational procedures in place, new opportunities, and prospective curriculum adjustments based on labour market realities. Due to the development of technology, businesses’ requirements for human resource credentials and job types underwent several modifications. This study’s goal is to investigate how COVID-19 has influenced curriculum revisions and employability requirements. The research used a mixed methodology, with quantitative analysis of changes in enrolled students by major and a qualitative study including two different sets of surveys based on the innovation and employability theories addressed to five human resource (HR) directors from institutions and organizations in Lebanon, as well as three universities. The objective was to answer the following questions: How can universities adapt to the changing demands of the labour market specifically in times of crisis? Should university curricula place greater emphasis on students’ personal growth than on technical and conventional learning? The results lead to re-thinking about what higher education systems and institutions can do to redesign their curricula in accordance with the job market and the expectations of the students in this challenging context, where employment security and job market stability issues are more urgent due to the economic crisis and advanced technologies. According to the findings, the research implications include boosting the implementation of the new curriculum through improved HR practices from the Ministry of Education. This will also encourage innovative performance, which will necessitate realistic, swift technical procedures to be unbeatable, creative, and competitive. This study adds significantly to the literature by suggesting curriculum adjustments for online courses and e-training.
Experimental Research on Reconstruction Techniques for Instantaneous Regression Rate of Hybrid Rocket Motor with Single-Port Wagon Wheel Fuel Grain
Authors: Wei Tianfang , Cai Guobiao , Tian Hui , Zhang Yuanjun , Li Chengen , Meng Xiangyu
Published: May 2023

This study investigated reconstruction techniques for building the instantaneous fuel regression rate of the hybrid rocket motor (HRM). Specifically, an experiment in a laboratory 500 N-class hybrid rocket motor with single-port wagon wheel fuel grain, operated with hydrogen peroxide (HP) and hydroxyl-terminated polybutadiene (HTPB) based fuel (including Al), was carried out. A piece of post-processing software was developed to reconstruct the instantaneous regression rate and other performance parameters of the HRM during the firing test. The results produced by the reconstruction techniques are in good agreement with experimental data obtained by traditional methods, with a maximum error of less than 5.75%. Moreover, compared with the traditional endpoint method, the reconstruction method had a significant advantage, which could ascertain the sensitivity of the regression rate to changes in the oxidizer mass flux and fit the formula of regression rate in a single firing test. Additionally, digital image processing techniques were employed to determine the axial distribution of the fuel regression rate after the test using computed tomography (CT) scanning. This served to verify the accuracy of the instantaneous reconstruction calculation. The error in the average regression rate between CT scanning and the reconstruction calculation was 1.91%, proving that the CT scanning and pixel statistic method of the calculating regression rate was practical for characterizing the axial distribution of the average regression rate during the firing test. In summary, the main objective of this study was to reconstruct the transient parameters of hybrid rocket motor with single-port wagon wheel fuel grain using reconstruction techniques, and to fit the formula of the regression rate through a single-firing test. Furthermore, this paper proposes a modified reconstruction method that is essential for investigating fuel regression rate during the firing test of HRMs.
Tea Tree Pest Detection Algorithm Based on Improved Yolov7-Tiny
Authors: Yang Zijia , Feng Hailin , Ruan Yaoping , Weng Xiang
Published: May 2023

Timely and accurate identification of tea tree pests is critical for effective tea tree pest control. We collected image data sets of eight common tea tree pests to accurately represent the true appearance of various aspects of tea tree pests. The dataset contains 782 images, each containing 1~5 different pest species randomly distributed. Based on this dataset, a tea garden pest detection and recognition model was designed using the Yolov7-tiny network target detection algorithm, which incorporates deformable convolution, the Biformer dynamic attention mechanism, a non-maximal suppression algorithm module, and a new implicit decoupling head. Ablation experiments were conducted to compare the performance of the models, and the new model achieved an average accuracy of 93.23%. To ensure the validity of the model, it was compared to seven common detection models, including Efficientdet, Faster Rcnn, Retinanet, DetNet, Yolov5s, YoloR, and Yolov6. Additionally, feature visualization of the images was performed. The results demonstrated that the Improved Yolov7-tiny model developed was able to better capture the characteristics of tea tree pests. The pest detection model proposed has promising application prospects and has the potential to reduce the time and economic cost of pest control in tea plantations.
Environmental and Economic Responses to Precision Feed Management in Dairy Cattle Diets
Authors: Chase Larry E. , Fortina Riccardo
Published: May 2023

The dairy industry is under pressure to reduce its environmental impact while producing more milk to meet society’s needs. Precision feed management (PFM) is one tool that can be used to meet this challenge. A simple definition of PFM is to provide adequate, but not excessive, nutrients to meet animal requirements. There has been an industry trend to decrease diet nitrogen (N) and phosphorus (P) in the last 20 years. New York herds lowered diet N by 10.8% between 1999 and 2019. Milk per cow increased by 40% during this time, while total manure N excretion decreased by 8.1%. Research results from on-farm trials have consistently indicated that lowering diet crude protein (CP) decreases manure N excretion while improving profitability; in a 3-year study with 8 herds, diet CP was decreased by 9.7%, while manure N excretion was 14% lower. Income over purchased feed cost increased by $137/cow/year. Carbon footprint (CF) has also decreased. A decrease of 19% between 2007 and 2017 was reported for the United States dairy industry. The dairy industry has already made significant strides in improving milk production while lowering its impact on the environment; however, there are additional opportunities to further reduce nutrient excretion and emissions into the environment and increase the efficiency of nutrient use.
Physiological and Biochemical Mechanisms of Exogenous Melatonin Regulation of Saline–Alkali Tolerance in Oats
Authors: Wang Qiang , Xu Weiwei , Ren Changzhong , Zhan Chao , Wang Chunlong , Li Junwei , Ren Qinyong , Liang Xiaotian , Wei Liming , Xiang Dabing , Wang Junying , Guo Laichun
Published: May 2023

Saline–alkali stress is one of the major factors limiting oat seed germination. The regulatory role of melatonin (MT) as a naturally occurring active substance is well known, but the mechanism of MT-mediated intrinsic physiological regulation of oat seed germination under saline–alkali stress is unclear. Therefore, this study investigated (1) the variability of different MT seed soaking concentrations and times on the germination of oat seeds under saline–alkali stress, and (2) the possible physiological regulatory mechanisms of MT on the germination of oat seeds under saline–alkali stress. The results showed that seed vigor was significantly reduced under saline–alkali stress, and seed germination of oats was significantly inhibited; different concentrations of MT seed soaking treatments improved the germination rate, germination potential, germination index, vigor index, root length, germ length, fresh weight, and dry weight; and, overall, treatment improved seed germination and exhibited the phenomenon of “low promotion and high inhibition”. The 100 μmol·L−1 MT seed soaking treatment effectively enhanced the activities of seed antioxidant enzymes (SOD, POD, CAT, and APX) and nonenzymatic antioxidants (GSH and AsA), affected the AsA-GSH cycle, and effectively increased the contents of osmoregulatory substances (proline, protein, and soluble sugar) by reducing levels of H2O2, OH−, and MDA, thus enhancing the tolerance of oats to saline–alkali and promoting seed germination. In conclusion, MT has a positive effect on the saline–alkali tolerance of oat seeds.
Phenological Growth Stages of Abelmoschus manihot: Codification and Description According to the BBCH Scale
Authors: Qian Wenzhang , Hu Yunyi , Lin Xi , Yu Deshui , Jia Shibing , Ye Yulin , Mao Yidong , Yi Lu , Gao Shun
Published: May 2023

Abelmoschus manihot L. (A. manihot) has received more and more attention due to its potential edible and medicinal value. It shows higher yield and related fine agronomic traits suitable for disadvantaged areas and low-input planting. However, a systemic description of the phenological growth stages of A. manihot, an alternative, multipurpose crop of worldwide interest, does not exist. This study aims to detail the phenological growth stages of A. manihot based on the BBCH scale. Nine principal growth stages were described from seed germination to senescence, along with 69 secondary growth stages, including germination, leaf development, formation of side shoots, primary stem elongation, inflorescence emergence, flowering, fruit development, maturation of fruit and seed, and senescence. However, the morphology and structure of A. manihot become complex with growth, and some growth stages, like inflorescence development, flowering, fruit development, and fruit maturation, overlap totally or partially with each other. Thus, the three-digit scale is considered necessary for a complementary description of these growth stages and illustrations for clarification. Moreover, the unique morphology and structure features of the pistil, stamen, and ovary and the development process of fruits and seeds were described in detail at different stages. The basic and extended BBCH scales will add new information on defining and identifying A. manihot phenological growth stages. They will help farmers efficiently schedule and manage A. manihot cultivation and improve knowledge dissemination among growers and researchers.
Response of Grain Yield to Planting Density and Maize Hybrid Selection in High Latitude China—A Multisource Data Analysis
Authors: Sun Shanwen , Huang Zhaofu , Liu Haiyan , Xu Jian , Zheng Xu , Xue Jun , Li Shaokun
Published: May 2023

Identifying the relationships between the yield of rainfed maize and planting densities as well as the hybrids used is crucial for ensuring the sustainable development of the grain industry in high latitude China. In this study, we collected 108 grain yield date points from our multiyear (2017–2020) field experiments and combined 213 data points collected from 21 published papers to appraise the impact of planting density and hybrids on maize yield. It was found that grain yield forms a curvilinear relationship with plant density as it increased from 22,500 to 112,500 plants ha−1. The optimum plant density (OPD) was determined to be 72500 plants ha−1, with a maximum maize grain yield of 10.56 Mg ha−1. The interannual variability in grain yields among hybrids with different planting densities was mainly due to the differences in dry matter (DM), especially post-silking. Grain yields increased significantly with a rise in the proportion of post-silking DM to DM at maturity. In addition, both the collected literature and our field experiments showed that the OPD was positively correlated with solar radiation accumulated during the maize growth period and with each hybrid’s year of release. This study suggests that increasing plant density and selecting new hybrids with suitable growth periods are effective approaches for increasing grain yield in high latitude China.
Utilizing NUtrack to Access the Activity Levels in Pigs with Varying Degrees of Genetic Potential for Growth and Feed Intake
Authors: Obermier Dalton , Trenahile-Grannemann Melanie , Schmidt Ty , Rathje Tom , Mote Benny
Published: May 2023

Feed cost accounts for over two-thirds of the variable cost of production. In order to reduce feed costs without sacrificing production numbers, feed efficiency must be improved. Calorie expenditure has been difficult to quantify in the past but is understood to impact residual feed intake (RFI) greatly. The objective of this work was to utilize an advanced computer vision system to evaluate activity levels across sex and sire groups with different expected breeding value combinations for growth and feed intake. A total of 199 pigs from four different sire groups (DNA Genetics Line 600) High Feed Intake/High Growth (HIHG), Low Feed Intake/High Growth (LIHG), High Feed Intake/Low Growth (HILG), and Low Feed Intake/Low Growth (LILG) were utilized at the UNL ENREC farm over 127 days. The NUtrack system allowed for individual monitoring of pigs in group housing to track daily activity traits. In total, HIHG pigs travelled less (p < 0.05; 139 vs. 150 km), spent more time lying (p < 0.05; 2421 vs. 2391 h), and less time eating (p < 0.05; 235 vs. 243 h) when compared to LILG pigs across time. The results suggest variation in activity occurs across the progeny of the sire groups selected to differentiate in growth and feed intake.
Brown and Green Seaweed Antioxidant Properties and Effects on Blood Plasma Antioxidant Enzyme Activities, Hepatic Antioxidant Genes Expression, Blood Plasma Lipid Profile, and Meat Quality in Broiler Chickens
Authors: Azizi Mohammad Naeem , Loh Teck Chwen , Foo Hooi Ling , Akit Henny , Izuddin Wan Ibrahim , Yohanna Danladi
Published: May 2023

The study was designed to analyze the effects of brown seaweed (BS) and green seaweed (GS) on blood plasma antioxidant enzyme activities, hepatic antioxidant genes expression, blood plasma lipid profile, breast meat quality, and chemical composition in broiler chickens. The dietary treatment groups contained basal diet [negative control (NC)], basal diet + vitamin E (100 mg/kg feed) [positive control (PC)], basal diet + 0.25, 0.50, 0.75, 1, and 1.25% BS and GS supplements separately. The findings showed that both BS and GS exhibited remarkable antioxidant activity. In contrast, the maximum antioxidant activity was recorded by BS (55.19%), which was significantly higher than the GS (25.74%). Results showed that various levels of BS and GS had no significant effects on broiler blood plasma catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) enzyme activities. The hepatic superoxide dismutase 1 (SOD1) gene mRNA expression was significantly higher for birds fed 0.50% and 0.75% BS. Regarding the plasma lipid profile, the total cholesterol (TC) and high-density lipoprotein (HDL) levels were higher (p < 0.05) for birds fed 0.75 and 1% BS compared to the negative and positive control groups. The findings showed that different levels of BS and GS had significantly higher breast meat crude protein (CP) content.
Ultrasound Evaluation of Extracranial Cerebral Circulation (The Common, External and Internal Carotid Artery) in Different Breeds of Dogs
Authors: Ševčíková Marieta K. , Figurová Mária , Ševčík Karol , Hluchý Marián , Domaniža Michal , Lapšanská Mária , Drahovská Zuzana , Žert Zdeněk
Published: May 2023

Noninvasive Doppler ultrasonography (US) examination is a standard method for the clinical evaluation of the carotid arteries. Extracranial cerebral circulation includes the common carotid artery (CCA), the external carotid artery (ECA), and the extracranial part of the internal carotid artery (ICA). The present study was conducted with the objective of assessing physiological normative values and describing the appearance of spectral waveforms of extracranial arteries in 104 healthy dogs of eight breeds divided into four groups according to weight. We also focused on searching for correlations for carotid blood velocities with the resistive index (RI), body weight and diameter, and differences between observers and the influence of sex in the calculations of Doppler parameters. In the evaluated breeds, significant differences were found in the velocity of peak systolic velocity (PSV) and end diastolic velocity (EDV). There was a strong correlation between body weight and peak systolic velocity, the RI index and CCA diameter. The intra-observer agreement for the PSV and EDV parameters in each vessel was considered excellent reliability, and overall interobserver agreement showed very good reliability. This study could improve the descriptions of physiological values and waveforms recorded in carotid arteries. Defining the physiological values of velocity and the resistive index (RI) helps in the easier identification of pathology and diagnosis of disease. Our results may be used for further studies of vascular diseases in veterinary medicine that correlate with the pathology of neurological disorders of ischemic origin, further in thromboembolism, oncologic disease and degenerative, proliferative and inflammatory diseases of the arteries that lead to stenosis
Domestic Pets in the United Arab Emirates as Reservoirs for Antibiotic-Resistant Bacteria: A Comprehensive Analysis of Extended-Spectrum Beta-Lactamase Producing Escherichia coli Prevalence and Risk Factors
Authors: Habib Ihab , Mohteshamuddin Khaja , Mohamed Mohamed-Yousif Ibrahim , Lakshmi Glindya Bhagya , Abdalla Afra , Bakhit Ali Alkaabi Abdulla
Published: May 2023

Extended-spectrum β-lactamases resistant (ESBL-R) Escherichia coli (E. coli) has been reported from healthy and sick pets. However, data from Middle Eastern countries, including the United Arab Emirates (UAE), are minimal. This study provides the first evidence of ESBL-R E. coli carriage among pets in the UAE. A total of 148 rectal swabs were collected from domestic cats (n = 122) and dogs (n = 26) attending five animal clinics in the UAE. Samples were cultured directly onto selective agar, and suspected colonies were confirmed as ESBL-producing using phenotypic and molecular methods. Confirmed isolates were screened for their phenotypic resistance to twelve antimicrobial agents using the Kirby Bauer method. The owners of the pets completed a questionnaire at the time of sampling, and the data were used to identify risk factors. ESBL-R E. coli was detected in rectal swabs of 35 out of 148 animals (23.65%) (95% confidence interval [CI]: 17.06–31.32). Multivariable logistic regression analysis identified cats and dogs with access to water in ditches and puddles as 3.71 (p-value = 0.020) times more likely to be positive to ESBL-R E. coli than those without access to open water sources. Ciprofloxacin resistance was evident in 57.14% (44/77) of the ESBL-R E. coli isolates. The percentage of resistance to azithromycin and cefepime was 12.99% (10/77) and 48.05% (37/77), respectively. The blaCTX-M gene was detected in 82% of the PCR-screened isolates (n = 50). Multidrug resistance (MDR) phenotypes were evident in 91% (70/77) of the isolates. In conclusion, ESBL-R E. coli was detected at a noticeable rate among healthy pet cats and dogs in the UAE, and the majority are MDR to clinically important antimicrobials such as fluoroquinolones and 3rd and 4th generation cephalosporins. Our results call for strengthening antimicrobial stewardship among companion animal veterinarians in the UAE to reduce the potential transmission of ESBL-R E. coli between pets, humans, and urban environments.
Genomic and Phenotypic Udder Evaluation for Dairy Cattle Selection: A Review
Authors: Gutiérrez-Reinoso Miguel A. , Aponte Pedro M. , García-Herreros Manuel
Published: May 2023

The traditional point of view regarding dairy cattle selection has been challenged by recent genomic studies indicating that livestock productivity prediction can be redefined based on the evaluation of genomic and phenotypic data. Several studies that included different genomic-derived traits only indicated that interactions among them or even with conventional phenotypic evaluation criteria require further elucidation. Unfortunately, certain genomic and phenotypic-derived traits have been shown to be secondary factors influencing dairy production. Thus, these factors, as well as evaluation criteria, need to be defined. Owing to the variety of genomic and phenotypic udder-derived traits which may affect the modern dairy cow functionality and conformation, a definition of currently important traits in the broad sense is indicated. This is essential for cattle productivity and dairy sustainability. The main objective of the present review is to elucidate the possible relationships among genomic and phenotypic udder evaluation characteristics to define the most relevant traits related to selection for function and conformation in dairy cattle. This review aims to examine the potential impact of various udder-related evaluation criteria on dairy cattle productivity and explore how to mitigate the adverse effects of compromised udder conformation and functionality. Specifically, we will consider the implications for udder health, welfare, longevity, and production-derived traits. Subsequently, we will address several concerns covering the application of genomic and phenotypic evaluation criteria with emphasis on udder-related traits in dairy cattle selection as well as its evolution from origins to the present and future prospects.
Human Injuries Associated with the Transport of Horses by Road
Authors: Riley Christopher B. , Padalino Barbara , Rogers Chris W. , Thompson Kirrilly R.
Published: May 2023

There is an increased understanding of shared human–animal risk in terms of “one welfare”, whereby when animals are at risk, so are people, so preventing injury to one species may also prevent injury to the other. Because transport-related injuries to horses are common, the authors considered this paradigm to study road equine transport-related injuries to humans in New Zealand. The aim was to determine their frequency and associated factors by distributing a survey to horse industry participants through industry organisations asking about their horse activities, road transport experiences, and any related self-injury. There were 112/1067 (10.5%) handlers injured while preparing (13/112), loading (39/112), traveling (6/112), or unloading (33/112). Of these, 40% had multiple injury types, and 33% had several body regions affected. Hand injuries were most common (46%), followed by the foot (25%), arm (17%), and head or face (15%). Median recovery time was 7 days. Injuries were associated with the responder’s industry educational background, years of driving experience, and reporting a horse injured during road transport in the past two years. Wearing helmets and gloves, and adopting strategies designed to eliminate equine injuries associated with the road transport of horses to reduce the risk of injury to their handlers are recommended.
Dietary Supplementation of Lactobacillus johnsonii RS-7 Improved Antioxidant and Immune Function of Weaned Piglets
Authors: Zhao Kai , Yin Heng , Yan Honglin , Tang Wenjie , Diao Hui , Wang Qi , Qi Renli , Liu Jingbo
Published: May 2023

We investigated the effects of dietary supplementation of lactic acid bacteria on the immune and antioxidant performance of weaned pigs. A total of 128 Duroc × Landrace × Yorkshire piglets weaned on day 28 with an average body weight of 8.95 ± 1.15 kg were selected and randomly divided into four treatment groups according to body weight and sex for a 28-day study. The four dietary treatments were basal diet (CON), and CON with 0.05% (LJ0.05), 0.1% (LJ0.1), and 0.2% (LJ0.2) Lactobacillus johnsonii RS-7, respectively. The lowest feed-to-gain ratio (F:G) was found when LJ0.1 was added to the diet. The addition of compound lactic acid bacteria to the diet increased the concentrations of TP, ALB, IgA, and IgM on day 14 and IgG, IgA, and IgM on day 28 (p < 0.05) in the blood, with trait values greater for pigs fed LJ0.1 than CON pigs (p < 0.05). Concentrations of antioxidants (CAT, T-AOC, MDA, T-SOD, and GSH) in serum, intestinal mucosa, spleen, liver, and pancreas improved. In summary, dietary supplementation of Lactobacillus johnsonii RS-7 improved the antioxidant and immune function of weaned piglets.
Clinical Efficacy of a Single Intravenous Regional Limb Perfusion with Marbofloxacin versus Ceftiofur Sodium to Treat Acute Interdigital Phlegmon in Dairy Cows
Authors: Celani Gianluca , Straticò Paola , Albano Paolo , Petrizzi Lucio , Mortellaro Carlo Maria , Varasano Vincenzo
Published: May 2023

The objective of the study was to compare the clinical efficacy of a single antimicrobial intravenous regional limb perfusion (IVRLP) with marbofloxacin versus ceftiofur sodium to treat naturally occurring interdigital phlegmon (IP) in dairy cows. The study had a randomized parallel-group design. Forty lactating Friesian cows clinically diagnosed with acute IP were enrolled, assigned to one of two treatment groups, and received a single IVRLP with the antimicrobial drug selected (M: 0.67 mg/kg of marbofloxacin; C: 500 mg/animal of ceftiofur sodium). Clinical data for the severity of lameness, digital swelling and local lesion appearance were assessed at diagnosis and at 5, 10 and 15 days post-IVRLP. Clinical resolution was defined as digital swelling disappearance, locomotion score reduction of at least 2/5 points, healed or healing local lesion and no relapse at 15 days after IVRLP. The total daily milk yield of each cow on the day before the clinical detection, on the day of diagnosis and on the day of the clinical follow-up post-IVRLP were registered. Lameness, digital swelling and local lesion severity were not significantly different between groups at any time-point. In both groups, on the fifteenth day after treatment, 17/20 (85%) cows showed a positive outcome, with no significant difference (p > 0.05). The daily milk production of all cows was adversely affected by the clinical onset of IP and gradually returned to a normal level after IVRLP in both groups. These preliminary results support the hypothesis that a single antimicrobial IVRLP procedure, irrespective of the antimicrobial selected (ceftiofur vs. marbofloxacin), has a high success rate and restores milk yield in cases of dairy cattle with acute IP lameness.
Ampicillin and Ceftobiprole Combination for the Treatment of Enterococcus faecalis Invasive Infections: “The Times They Are A-Changin”
Authors: Giuliano Simone , Angelini Jacopo , D’Elia Denise , Geminiani Monica , Barison Roberto Daniele , Giacinta Alessandro , Sartor Assunta , Campanile Floriana , Curcio Francesco , Cotta Menino Osbert , Roberts Jason A. , Baraldo Massimo , Tascini Carlo
Published: May 2023

Background: Enterococcus faecalis is responsible for a large variety of severe infections. This study is a case series reporting our experience in the treatment of E. faecalis invasive infections with ampicillin in combination with ceftobiprole (ABPR). Methods: We retrospectively analyzed all the medical records of patients admitted to the University Hospital of Udine from January to December 2020 with a diagnosis of infective endocarditis or primary or non-primary complicated or uncomplicated bacteremia caused by E. faecalis. Results: Twenty-one patients were included in the final analysis. The clinical success rate was very high, accounting for 81% of patients, and microbiological cure was obtained in 86% of patients. One relapse was recorded in one patient who did not adhere to the partial oral treatment prescribed. Therapeutic drug monitoring (TDM) was always performed for ampicillin and ceftobiprole, and serum concentrations of both drugs were compared to the MICs of the different enterococcal isolates. Conclusions: ABPR is a well-tolerated antimicrobial regimen with anti-E. faecalis activity. TDM can help clinicians optimize medical treatments to achieve the best possible efficacy with fewer side effects. ABPR might be a reasonable option for the treatment of severe invasive infections caused by E. faecalis due to the high level of enterococcal penicillin-binding protein (PBP) saturation.
Anti-Candidal Activity of Reboxetine and Sertraline Antidepressants: Effects on Pre-Formed Biofilms
Authors: Ahmed Eman Ibrahim , Alhuwaydi Ahmed M. , Taha Ahmed E. , Abouelkheir Mohamed
Published: May 2023

Reboxetine (REB) and sertraline (SER) are antidepressants. The antifungal potential of these drugs against planktonic Candida has been recently reported with limited data about their effects on Candidal biofilms. Biofilms are self-derived extracellular matrixes produced by the microbial population that is attached to biotic surfaces, such as vaginal and oral mucosa, or abiotic surfaces, such as biomedical devices, resulting in persistent fungal infections. The commonly prescribed antifungals, azoles, are usually less effective when biofilms are formed, and most of the prescribed antifungals are only fungistatic. Therefore, the current study investigates the antifungal potentials of REB and SER, alone and in combination with fluconazole (FLC) and itraconazole (ITR) against Candidal biofilms. Using proper controls, Candida species (Candida albicans, C. albicans; Candida krusei, C. krusei; and Candida glabrata, C. glabrata) were used to form biofilms in 96-well microplates. Serial dilutions corresponding to concentrations ranging from 2 to 4096 µg/mL of the target drugs (REB, SER, FLC, ITR) were prepared and added to the plates. Impairment of the biofilm biomass and biofilm metabolic viability was detected using the crystal violet (CV) assay and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, respectively. In the checkerboard assay, the sessile fractional inhibitory concentration index (SFICI) was calculated to evaluate the effects of drug combinations. SER was more effective in reducing the biomass than REB for C. albicans and C. glabrata, but both were equal for C. krusei. For the reduction in metabolic activity in C. albicans and C. glabrata, SER had a slight advantage over REB. In C. krusei, REB was slightly more potent. Overall, FLC and ITR were almost equal and produced more significant reductions in metabolic activity when compared to SER and REB, except for C. glabrata, where SER was almost equal to FLC. Synergism was detected between REB + FLC and REB + ITR against biofilm cells of C. albicans. Synergism was detected between REB + ITR against biofilm cells of C. krusei. Synergism was detected between REB + FLC and REB + ITR against biofilm cells of C. albicans, C. krusei, and C. glabrata. The results of the present study support the potential of SER and REB as anti-Candidal biofilm agents that are beneficial as a new antifungal to combat Candidal resistance.
Editorial of the Special Issue “Advanced Robotics Applications in Industry”
Authors: Papakostas Nikolaos , Wang Lihui , Makris Sotiris , Mourtzis Dimitris
Published: May 2023

Recently, the emergence of various technological advancements has enabled the development of new and unique applications for industrial robots [...]
Determinants of Cyberattack Prevention in UAE Financial Organizations: Assessing the Mediating Role of Cybersecurity Leadership
Authors: Al-Kumaim Nabil Hasan , Alshamsi Sultan Khalifa
Published: May 2023

Cyberattack prevention factors have a significant impact on the perception of social and moral values in the business context. Despite leaders’ significant role in encouraging and enculturating cybersecurity practices in their organizations, there is a noticeable gap in the literature to highlight empirically how leaders and top management in organizations foster organizational cybersecurity. Therefore, this study aims to explore the role of cybersecurity leadership in financial organizations in preventing cyberattacks and investigate other human and non-technical factors related to the individual in financial organizations. Based on Protection Motivation Theory (PMT), the research framework was developed with the tallying of new variables focusing on the role of an organization’s cybersecurity leadership, training frequency, and the role of government frequent alerting. This research employed a quantitative research method. The data were collected through a questionnaire from 310 financial executive officers from selected banks in UAE that use digital technology to enhance their daily banking operations. Using Structural Equation Modelling (SEM), the results indicated (1) a significant association between all investigated independent variables and cybersecurity leadership through hypothesis (H8–H14); (2) cybersecurity leadership mediates the relationship between investigated independent variables and cyberattack prevention, from hypothesis (H15, and H16–H22); (3) no significant association between investigated independent variables and cyberattack prevention from hypothesis (H1–H6), except hypothesis (H4 and H7), which show a significant association. The coefficient of cybersecurity leadership in this study is viewed as a prevention element against cyberattacks based on the findings. With greater cybersecurity leadership success, the implementation of cyberattack prevention increases. This study emphasizes the importance of cybersecurity leadership in a cyberspace environment that protects against cyberattacks and promotes cybersecurity awareness within financial organizations and society in UAE.