bluuebunny/crossref_metadata_2025 huggingface dataset:
Methodology for Assessing Tractor Traction Properties with Instability of Coupling Weight
Authors: Lebedev Anatoliy , Shuliak Mykhailo , Khalin Stanislav , Lebedev Sergei , Szwedziak Katarzyna , Lejman Krzysztof , Niedbała Gniewko , Łusiak Tomasz
Published: April 2023

The purpose of the study is to increase the efficiency of using the tractor hitch weight in traction mode by reducing the uneven distribution of vertical reactions between the wheels. This work is grounded on a methodology that involves summarizing and analyzing established scientific findings related to the theory of tractors operating in traction mode. The analytical method and comparative analysis were employed to establish a scientific problem, define research objectives, and achieve the goal. The key principles of probability theory were applied in developing the empirical models of the tractor. The main provisions of the methodology for evaluating the traction properties of the tractor with the instability of the coupling weight were formulated. The method of evaluating the vertical reactions on the wheels of the tractor is substantiated, which is based on the measurement of the vertical reaction on one of the four wheels. It was proven that tractors with a center of mass offset to the front or rear axles have the greatest probability of equal distribution of vertical reactions between the wheels of one axle, and tractors with a center of mass in the middle between the axles have the lowest probability. It is theoretically substantiated and experimentally confirmed that when the tractor performs plowing work with uneven distribution of loads on the sides, its operation with maximum traction efficiency is ensured by blocking the front and rear axle drivers.
An Effective Hybrid Fungicide Containing Tea Tree Oil and Difenoconazole for Grape Powdery Mildew Management
Authors: Reuveni Moshe , Arroyo Cristobal J. , Ovadia Shmuel
Published: April 2023

Grape powdery mildew caused by Erysiphe necator (Schw.) Burr. is a destructive disease in vineyards. Synthetic fungicides are the main tool to combat this disease. The search for new alternatives to reduce pesticide usage and tactical approaches for resistance management encouraged us to develop the novel strategy that we report here. We evaluated the efficacy of a new premixed hybrid fungicide containing the demethylation inhibitor (DMI) difenoconazole and essential tea tree oil (TTO), derived from the Melaleuca alternifolia plant, against grape powdery mildew in seven field trials and two large-scale demonstration trials conducted in two different regions in the world, including Chile and Israel. Foliar sprays of difenoconazole-TTO were applied as a preventive treatment in field trials at 40–80 up to 80–160 gr/ha active ingredient, and they were highly effective in controlling powdery mildew on the fruit clusters of both wine and table grapes in experimental and large-scale demonstration trials and provided up to 99% efficacy in disease incidence and severity compared with the untreated control. Difenoconazole-TTO was as or more effective than other DMI fungicides, including difenoconazole, a pre-mixed fungicide boscalid-pyraclostrobin, or treatments that included various fungicides applied in rotation or mixtures of fungicides. The results suggest that a combination of difenoconazole-TTO with a reduced synthetic chemical load can be included in powdery mildew control programs for grapevine as a strategic approach in fungicide resistance management in vineyards.
Development of a Novel Anti-CD44 Variant 5 Monoclonal Antibody C44Mab-3 for Multiple Applications against Pancreatic Carcinomas
Authors: Kudo Yuma , Suzuki Hiroyuki , Tanaka Tomohiro , Kaneko Mika K. , Kato Yukinari
Published: April 2023

Pancreatic cancer exhibits a poor prognosis due to the lack of early diagnostic biomarkers and the resistance to conventional chemotherapy. CD44 has been known as a cancer stem cell marker and plays tumor promotion and drug resistance roles in various cancers. In particular, the splicing variants are overexpressed in many carcinomas and play essential roles in the cancer stemness, invasiveness or metastasis, and resistance to treatments. Therefore, the understanding of each CD44 variant’s (CD44v) function and distribution in carcinomas is essential for the establishment of CD44-targeting tumor therapy. In this study, we immunized mice with CD44v3–10-overexpressed Chinese hamster ovary (CHO)-K1 cells and established various anti-CD44 monoclonal antibodies (mAbs). One of the established clones (C44Mab-3; IgG1, kappa) recognized peptides of the variant-5-encoded region, indicating that C44Mab-3 is a specific mAb for CD44v5. Moreover, C44Mab-3 reacted with CHO/CD44v3–10 cells or pancreatic cancer cell lines (PK-1 and PK-8) by flow cytometry. The apparent KD of C44Mab-3 for CHO/CD44v3–10 and PK-1 was 1.3 × 10−9 M and 2.6 × 10−9 M, respectively. C44Mab-3 could detect the exogenous CD44v3–10 and endogenous CD44v5 in Western blotting and stained the formalin-fixed paraffin-embedded pancreatic cancer cells but not normal pancreatic epithelial cells in immunohistochemistry. These results indicate that C44Mab-3 is useful for detecting CD44v5 in various applications and is expected to be useful for the application of pancreatic cancer diagnosis and therapy.
Resistance to Ceftazidime/Avibactam in Klebsiella pneumoniae KPC-Producing Isolates: A Real-Life Observational Study
Authors: Campogiani Laura , Vitale Pietro , Lodi Alessandra , Imeneo Alessandra , Fontana Carla , D’Agostini Cartesio , Compagno Mirko , Coppola Luigi , Spalliera Ilaria , Malagnino Vincenzo , Teti Elisabetta , Iannetta Marco , Andreoni Massimo , Sarmati Loredana
Published: April 2023

Background: Ceftazidime/avibactam (CAZ-AVI) resistance amongst Enterobacterales is worryingly increasing worldwide. Objectives: The aim of this study was to collect and describe real-life data on CAZ-AVI-resistant Klebsiella pneumoniae (KP) isolates in our University Hospital, with the ultimate goal of evaluating possible risk factors related to the acquisition of resistance. Methods: This is a retrospective observational study, including unique Klebsiella pneumoniae (KP) isolates resistant to CAZ-AVI (CAZ-AVI-R) and producing only KPC, collected from July 2019 to August 2021 at Policlinico Tor Vergata, Rome, Italy. The pathogen’s list was obtained from the microbiology laboratory; clinical charts of the corresponding patients were reviewed to collect demographic and clinical data. Subjects treated as outpatients or hospitalized for <48 h were excluded. Patients were then divided into two groups: S group, if they had a prior isolate of CAZ-AVI-susceptible KP-KPC, and R group, if the first documented isolate of KP-KPC was resistant to CAZ-AVI. Results: Forty-six unique isolates corresponding to 46 patients were included in the study. The majority of patients (60.9%) were hospitalized in an intensive care unit, 32.6% in internal medicine wards and 6.5% in surgical wards. A total of 15 (32.6%) isolates were collected from rectal swabs, representing a colonization. Amongst clinically relevant infections, pneumonia and urinary tract infections were the most commonly found (5/46, 10.9% each). Half of the patients received CAZ-AVI prior to isolation of the KP-KPC CAZ-AVI-R (23/46). This percentage was significantly higher in patients in the S group compared to patients in the R group (69.3% S group vs. 25% R group, p = 0.003). No differences between the two groups were documented in the use of renal replacement therapy or in the infection site. The clinically relevant CAZ-AVI-R KP infections (22/46, 47.8%) were all treated with a combination therapy, 65% including colistin and 55% including CAZ-AVI, with an overall clinical success of 38.1%. Conclusions: Prior use of CAZ-AVI was associated with the emergence of drug resistance.
Class III Peroxidases (POD) in Pepper (Capsicum annuum L.): Genome-Wide Identification and Regulation during Nitric Oxide (NO)-Influenced Fruit Ripening
Authors: González-Gordo Salvador , Muñoz-Vargas María A. , Palma José M. , Corpas Francisco J.
Published: April 2023

The class III peroxidases (PODs) catalyze the oxidation of several substrates coupled to the reduction of H2O2 to water, and play important roles in diverse plant processes. The POD family members have been well-studied in several plant species, but little information is available on sweet pepper fruit physiology. Based on the existing pepper genome, a total of 75 CaPOD genes have been identified, but only 10 genes were found in the fruit transcriptome (RNA-Seq). The time-course expression analysis of these genes showed that two were upregulated during fruit ripening, seven were downregulated, and one gene was unaffected. Furthermore, nitric oxide (NO) treatment triggered the upregulation of two CaPOD genes whereas the others were unaffected. Non-denaturing PAGE and in-gel activity staining allowed identifying four CaPOD isozymes (CaPOD I-CaPOD IV) which were differentially modulated during ripening and by NO. In vitro analyses of green fruit samples with peroxynitrite, NO donors, and reducing agents triggered about 100% inhibition of CaPOD IV. These data support the modulation of POD at gene and activity levels, which is in agreement with the nitro-oxidative metabolism of pepper fruit during ripening, and suggest that POD IV is a target for nitration and reducing events that lead to its inhibition.
Mangiferin Affects Melanin Synthesis by an Influence on Tyrosinase: Inhibition, Mechanism of Action and Molecular Docking Studies
Authors: Hering Anna , Stefanowicz-Hajduk Justyna , Dziomba Szymon , Halasa Rafal , Krzemieniecki Radoslaw , Sappati Subrahmanyam , Baginski Maciej , Ochocka Jadwiga Renata
Published: April 2023

Mangiferin is a strong antioxidant that presents a wide range of biological activities. The aim of this study was to evaluate, for the first time, the influence of mangiferin on tyrosinase, an enzyme responsible for melanin synthesis and the unwanted browning process of food. The research included both the kinetics and molecular interactions between tyrosinase and mangiferin. The research proved that mangiferin inhibits tyrosinase activity in a dose-dependent manner with IC50 290 +/− 6.04 µM, which was found comparable with the standard kojic acid (IC50 217.45 +/− 2.54 µM). The mechanism of inhibition was described as mixed inhibition. The interaction between tyrosinase enzyme and mangiferin was confirmed with capillary electrophoresis (CE). The analysis indicated the formation of two main, and four less significant complexes. These results have also been supported by the molecular docking studies. It was indicated that mangiferin binds to tyrosinase, similarly to L-DOPA molecule, both in the active center and peripheral site. As it was presented in molecular docking studies, mangiferin and L-DOPA molecules can interact in a similar way with surrounding amino acid residues of tyrosinase. Additionally, hydroxyl groups of mangiferin may interact with amino acids on the tyrosinase external surface causing non-specific interaction.
Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress
Authors: Ren Wenji , Wang Zixu , Cao Jing , Dong Yulan , Wang Tuanjie , Chen Yaoxing
Published: April 2023

Excessive illumination is one of the most severe environmental factors that impacts the organism. There is growing evidence that obesity significantly contributes to the onset of chronic kidney disease. However, the effect of continuous light on the kidney and which color can produce an apparent phenomenon remains elusive. In this study, C57BL/6 mice given either a normal diet (LD-WN) or a high-fat diet (LD-WF) were subjected to a light cycle of 12 h of illumination followed by 12 h of darkness for 12 weeks. Meanwhile, 48 high-fat diet mice were given a 24 h monochromatic light exposure of varying colors (white, LL-WF; blue, LL-BF; green, LL-GF) for 12 weeks. As expected, the LD-WF mice showed significant obesity, kidney injury, and renal dysfunction compared with the LD-WN group. LL-BF mice had worse kidney injury than LD-WF mice, including higher Kim-1 and Lcn2. The kidney of the LL-BF group showed marked glomerular and tubular injury, with decreased levels of Nephrin, Podocin, Cd2ap, and α-Actinin-4 compared to LD-WF. LL-BF also reduced the antioxidant capacity, including GSH-Px, CAT, and T-AOC, increased the production of MDA, and inhibited the activation of the NRF2/HO-1 signaling pathway. Furthermore, LL-BF upregulated the mRNA levels of the pro-inflammatory factors Tnf-α, Il-6, and Mcp-1, decreasing the inhibitory inflammatory Il-4 expression. We observed increased plasma corticosterone (CORT), renal glucocorticoid receptors (GR) expression, Hsp90, Hsp70, and P23 mRNA levels. These findings suggested that LL-BF increased CORT secretion and affected glucocorticoid receptors (GR) in comparison to the LD-WF group. Moreover, in vitro research demonstrated that CORT treatment increased oxidative stress and inflammation, which was counteracted by adding a GR inhibitor. Thus, the sustained blue light worsened kidney damage, possibly by inducing elevated CORT and increasing oxidative stress and inflammation via GR.
Activation of NRF2 Signaling Pathway Delays the Progression of Hyperuricemic Nephropathy by Reducing Oxidative Stress
Authors: Qiao Panshuang , Sun Yi , Wang Yiming , Lin Simei , An Yongpan , Wang Liang , Liu Jihan , Huang Yajun , Yang Baoxue , Zhou Hong
Published: April 2023

Hyperuricemia (HUA)-induced oxidative stress is a crucial contributor to hyperuricemic nephropathy (HN), but the molecular mechanisms underlying the disturbed redox homeostasis in kidneys remain elusive. Using RNA sequencing, together with biochemical analyses, we found that nuclear factor erythroid 2-related factor 2 (NRF2) expression and nuclear localization levels were increased in early HN progression and then gradually declined below the baseline level. We identified the impaired activity of the NRF2-activated antioxidant pathway as a driver of oxidative damage in HN progression. Through nrf2 deletion, we further confirmed aggravated kidney damage in nrf2 knockout HN mice compared with HN mice. In contrast, the pharmacological agonist of NRF2 improved kidney function and alleviated renal fibrosis in mice. Mechanistically, the activation of NRF2 signaling reduced oxidative stress by restoring mitochondrial homeostasis and reducing NADPH oxidase 4 (NOX4) expression in vivo or in vitro. Moreover, the activation of NRF2 promoted the expression levels of heme oxygenase 1 (HO-1) and quinone oxidoreductase 1 (NQO1) and enhanced the antioxidant capacity of cells. Furthermore, the activation of NRF2 ameliorated renal fibrosis in HN mice through the downregulation of the transforming growth factor-beta 1 (TGF-β1) signaling pathway and ultimately delayed the progression of HN. Collectively, these results suggested NRF2 as a key regulator in improving mitochondrial homeostasis and fibrosis in renal tubular cells by reducing oxidative stress, upregulating the antioxidant signaling pathway, and downregulating the TGF-β1 signaling pathway. The activation of NRF2 represents a promising strategy to restore redox homeostasis and combat HN.
Osthole Prevents Heart Damage Induced by Diet-Induced Metabolic Syndrome: Role of Fructokinase (KHK)
Authors: García-Arroyo Fernando E. , Gonzaga-Sánchez Guillermo , Silva-Palacios Alejandro , Roldán Francisco Javier , Loredo-Mendoza María L. , Alvarez-Alvarez Yamnia Quetzal , de los Santos Coyotl Jesus A. , Vélez Orozco Kevin A. , Tapia Edilia , Osorio-Alonso Horacio , Arellano-Buendía Abraham S. , Sánchez-Gloria José L. , Lanaspa Miguel A. , Johnson Richard J. , Sánchez-Lozada Laura Gabriela
Published: April 2023

There is increasing evidence that either ingested or produced fructose may have a role in metabolic syndrome. While not commonly considered a criterion for metabolic syndrome, cardiac hypertrophy is often associated with metabolic syndrome, and its presence carries increased cardiovascular risk. Recently it has been shown that fructose and fructokinase C (KHK) can be induced in cardiac tissue. Here we tested whether diet-induced metabolic syndrome causes heart disease associated with increased fructose content and metabolism and whether it can be prevented with a fructokinase inhibitor (osthole). Male Wistar rats were provided a control diet (C) or high fat/sugar diet for 30 days (MS), with half of the latter group receiving osthol (MS+OT, 40 mg/kg/d). The Western diet increased fructose, uric acid, and triglyceride concentrations in cardiac tissue associated with cardiac hypertrophy, local hypoxia, oxidative stress, and increased activity and expression of KHK in cardiac tissue. Osthole reversed these effects. We conclude that the cardiac changes in metabolic syndrome involve increased fructose content and its metabolism and that blocking fructokinase can provide cardiac benefit through the inhibition of KHK with modulation of hypoxia, oxidative stress, hypertrophy, and fibrosis.
Protective Effects of H2S Donor Treatment in Experimental Colitis: A Focus on Antioxidants
Authors: Török Szilvia , Almási Nikoletta , Veszelka Médea , Börzsei Denise , Szabó Renáta , Varga Csaba
Published: April 2023

Inflammatory bowel diseases (IBD) are chronic, inflammatory disorders of the gastrointestinal (GI) system, which have become a global disease over the past few decades. It has become increasingly clear that oxidative stress plays a role in the pathogenesis of IBD. Even though several effective therapies exist against IBD, these might have serious side effects. It has been proposed that hydrogen sulfide (H2S), as a novel gasotransmitter, has several physiological and pathological effects on the body. Our present study aimed to investigate the effects of H2S administration on antioxidant molecules in experimental rat colitis. As a model of IBD, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was used intracolonically (i.c.) to induce colitis in male Wistar–Hannover rats. Animals were orally treated (2 times/day) with H2S donor Lawesson’s reagent (LR). Our results showed that H2S administration significantly decreased the severity of inflammation in the colons. Furthermore, LR significantly suppressed the level of oxidative stress marker 3-nitrotyrosine (3-NT) and caused a significant elevation in the levels of antioxidant GSH, Prdx1, Prdx6, and the activity of SOD compared to TNBS. In conclusion, our results suggest that these antioxidants may offer potential therapeutic targets and H2S treatment through the activation of antioxidant defense mechanisms and may provide a promising strategy against IBD.
Influence of Gender Composition in Pedestrian Single-File Experiments
Authors: Paetzke Sarah , Boltes Maik , Seyfried Armin
Published: April 2023

Various studies address the question of which factors are relevant to the course of the fundamental diagram in single-file experiments. Some indicate that there are differences due to group composition when gender is taken into account. For this reason, further single-file experiments with homogeneous and heterogeneous group compositions were conducted. A Tukey HSD test was performed to investigate whether there are differences between the mean of velocity in different density ranges. A comparison of different group compositions showed that the effect of gender can only be seen, if at all, in a small density interval. Regression analyses were also conducted to determine whether, at high densities, the distance between individuals depends on the gender of the neighboring pedestrians and to establish which human factors have an effect on the velocity. An analysis of the distances between individuals at high densities indicated that there was no effect of the gender of the neighboring pedestrians. Taking into account additional human factors in a regression analysis did not improve the model.
A Study on Combustion Characteristics of Insensitive Triple-Base Propellant
Authors: Yang Yilan , Zhu Tianyi , Yan Zhiyu , Li Qianqian , Liu Bo , Wang Jinhua , Huang Zuohua
Published: April 2023

Research on combustion characteristics can provide basic information and theoretical support for the design of insensitive propellant. This work aims to investigate the combustion characteristics of insensitive triple-base propellant. All propellants were prepared based on same triple-base propellant, but they were desensitized with the same desensitizer in different ways. The high-speed camera, spontaneous luminescence, NO, NH chemiluminescence, and OH-planar laser induction fluorescence (PLIF) methods were employed to capture the combustion flame and derive the distributions of important intermediates. Results show that ignition delay times of insensitive propellants are obviously longer. This indicated that the application of the desensitizer has a partly hindering effect on the early ignition stage. The combustion time of insensitive propellants is mostly similar, which means that the desensitizer has little influence on the intensity of actual combustion. The change in flame height and area of insensitive propellants over time indicated that the combustion progressivity of some insensitive propellants was more prominent, which means that the desensitizer concentration and desensitizing methods all affect the performance of insensitive propellant. The signal intensities of NO and NH show a negative correlation, indicating that a competitive relationship probably exists between the formation of NO and NH radicals during the reaction process. The high concentration of OH mainly locates outside NO, suggesting that there may be a transformation between NO and OH. The maximum signal intensity of NO and NH of different insensitive propellants confirmed that both the concentration of desensitizers and the desensitizing methods exhibit important effect on the reaction process.
Diagenetic Study of Marrón Emperador Ornamental Stone (Upper Cretaceous, SE Spain)
Authors: Muñoz-Cervera María Concepción , Cañaveras Juan Carlos
Published: April 2023

The “Marrón Emperador” ornamental stone is known for its characteristic deep brown colour filled with white spots and veins. It consists of a brecciated dolostone with different generations of calcite/dolomite veins and veinlets that represent repeated episodes of fracture opening and partial or complete cementation, which likely corresponds to individual stages of fluid expulsion. Mineralogical, petrographic and geochemical studies point to the formation of these rocks through brecciation, dolomitization and rapid cementation processes in an active tectonic regimen. The composition and textural features of the stratiform dolomite geobody point to a structurally controlled dolomitization model. The overall breccia geometry, breccia texture and vein characteristics are all consistent with a brecciation origin driven by hydraulic fracturing, with subsequent calcite precipitation in open space and partial solution replacement of clasts. A paragenetic sequence includes: (1) marine sedimentation of original tidal carbonate sediments; (2) early lithification and marine cementation; (3) burial diagenesis with early fracturation of limestones; (4) entrance of dolomitizing fluids through fractures causing pervasive dolomitization (brown dolostones) and dolomite cements (fracture-lining and saddle dolomites); (5) fracturation by hydraulic overpressure under an active tectonic regime; (6) calcite cementation (white veins and veinlets); and (7) uplift and meteoric diagenesis producing dedolomitization, karstification and local brecciation.
An Overview of Open Source Deep Learning-Based Libraries for Neuroscience
Authors: Tshimanga Louis Fabrice , Del Pup Federico , Corbetta Maurizio , Atzori Manfredo
Published: April 2023

In recent years, deep learning has revolutionized machine learning and its applications, producing results comparable to human experts in several domains, including neuroscience. Each year, hundreds of scientific publications present applications of deep neural networks for biomedical data analysis. Due to the fast growth of the domain, it could be a complicated and extremely time-consuming task for worldwide researchers to have a clear perspective of the most recent and advanced software libraries. This work contributes to clarifying the current situation in the domain, outlining the most useful libraries that implement and facilitate deep learning applications for neuroscience, allowing scientists to identify the most suitable options for their research or clinical projects. This paper summarizes the main developments in deep learning and their relevance to neuroscience; it then reviews neuroinformatic toolboxes and libraries collected from the literature and from specific hubs of software projects oriented to neuroscience research. The selected tools are presented in tables detailing key features grouped by the domain of application (e.g., data type, neuroscience area, task), model engineering (e.g., programming language, model customization), and technological aspect (e.g., interface, code source). The results show that, among a high number of available software tools, several libraries stand out in terms of functionalities for neuroscience applications. The aggregation and discussion of this information can help the neuroscience community to develop their research projects more efficiently and quickly, both by means of readily available tools and by knowing which modules may be improved, connected, or added.
Forecasting Albacore (Thunnus alalunga) Fishing Grounds in the South Pacific Based on Machine Learning Algorithms and Ensemble Learning Model
Authors: Zhang Jie , Fan Donlin , He Hongchang , Xiao Bin , Xiong Yuankang , Shi Jinke
Published: April 2023

To achieve high-precision forecasting of different grades of albacore fishing grounds in the South Pacific Ocean, we used albacore fishing data and marine environmental factors data from 2009 to 2019 as data sources. An ensemble learning model (ELM) for albacore fishing grounds forecasting was constructed based on six machine learning algorithms. The overall accuracy (ACC), fishing ground forecast precision (P) and recall (R) were used as model accuracy evaluation metrics, to compare and analyze the accuracy of different machine learning algorithms for fishing grounds forecasting. We also explored the forecasting capability of the ELM for different grades of fishing grounds. A quantitative evaluation of the effects of different marine environmental factors on the forecast accuracy of albacore tuna fisheries was conducted. The results of this study showed the following: (1) The ELM achieved high accuracy forecasts of albacore fishing grounds (ACC = 86.92%), with an overall improvement of 4.39~19.48% over the machine learning models. (2) A better forecast accuracy (R2 of 81.82–98%) for high-yield albacore fishing grounds and a poorer forecast accuracy (R1 of 47.37–96.15%) for low-yield fishing grounds were obtained for different months based on the ELM; the high-yield fishing grounds were distributed in the sea south of 10° S. (3) A feature importance analysis based on RF found that latitude (Lat) had the greatest influence on the forecast accuracy of albacore tuna fishing grounds of different grades from February to December (0.377), and Chl-a had the greatest influence on the forecast accuracy of albacore tuna fishing grounds of different grades in January (0.295), while longitude (Lon) had the smallest effect on the forecast of different grades of fishing grounds (0.037).
The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum
Authors: Bogusławska-Wąs Elżbieta , Dłubała Alicja , Sawicki Wojciech , Ożgo Małgorzata , Lepczyński Adam
Published: April 2023

The main problem in processing bovine colostrum is preserving as many beneficial compounds as possible, most of which have low thermal stability. The present study evaluates the possibility of using cold plasma (CP) as a decontamination technology and its effect on selected biologically active fractions of freeze-dried bovine colostrum. The plasma process was carried out in air, nitrogen, and oxygen environments. The results revealed that the sterilization process using CP caused slight changes in the colour of the samples expressed by the attributes ΔC, ΔL, Δh and ΔE. The decontamination effect depended on the gas used and the type of microorganism. The highest decontamination effects were gained under oxygen conditions, where reductions were obtained for total psychrophilic bacteria (THPC) by log 1.24, mesophilic bacteria (THMC) by log 1.02, Enterobacteriaceae by log 1.16, E. coli by log 0.96, yeast (TYMC) by log 0.92. A significantly lower decontaminating effect was obtained for Gram-positive bacteria and sporophytic forms. Additionally, the application of CP, regardless of the gas used, affected the modification of protein structure and reduction of immunoglobulin concentration. as proven by proteomics analyses (1-DE, 2-DE, MALDI–TOF MS). The same applied to β-lactoglobulin in air and oxygen and BSA in nitrogen and air.
Impacts of Development, Dentofacial Disharmony, and Its Surgical Correction on Speech: A Narrative Review for Dental Professionals
Authors: Bode Christine , Ghaltakhchyan Nare , Rezende Silva Erika , Turvey Timothy , Blakey George , White Raymond , Mielke Jeff , Zajac David , Jacox Laura
Published: April 2023

Speech is a communication method found only in humans that relies on precisely articulated sounds to encode and express thoughts. Anatomical differences in the maxilla, mandible, tooth position, and vocal tract affect tongue placement and broadly influence the patterns of airflow and resonance during speech production. Alterations in these structures can create perceptual distortions in speech known as speech sound disorders (SSDs). As craniofacial development occurs, the vocal tract, jaws, and teeth change in parallel with stages of speech development, from babbling to adult phonation. Alterations from a normal Class 1 dental and skeletal relationship can impact speech. Dentofacial disharmony (DFD) patients have jaw disproportions, with a high prevalence of SSDs, where the severity of malocclusion correlates with the degree of speech distortion. DFD patients often seek orthodontic and orthognathic surgical treatment, but there is limited familiarity among dental providers on the impacts of malocclusion and its correction on speech. We sought to review the interplay between craniofacial and speech development and the impacts of orthodontic and surgical treatment on speech. Shared knowledge can facilitate collaborations between dental specialists and speech pathologists for the proper diagnosis, referral, and treatment of DFD patients with speech pathologies.
A Numerically Efficient Method to Assess the Elastic–Plastic Strain Energy Density of Notched and Imperfective Cast Steel Components
Authors: Horvath Michael , Oberreiter Matthias , Stoschka Michael
Published: April 2023

The fatigue strength of cast steel components is severely affected by manufacturing process-based bulk and surface imperfections. As these defect structures possess an arbitrary spatial shape, the utilization of local assessment methods is encouraged to design for service strength. This work applies the elastic–plastic strain energy density concept to study the fatigue strength properties of a high-strength cast steel alloy G12MnMo7-4+QT. A fatigue design limit curve is derived based on non-linear finite element analyses which merges experimental high-cycle fatigue results of unnotched and notched small-scale specimens tested at three different stress ratios into a unique narrow scatter band characterized by a scatter index of 1:TΔW¯(t)=2.43. A comparison to the linear–elastic assessment conducted in a preceding study reveals a significant improvement in prediction accuracy which is assigned to the consideration of the elastic–plastic material behaviour. In order to reduce computational effort, a novel approximation is presented which facilitates the calculation of the elastic–plastic strain energy density based on linear–elastic finite element results and Neuber’s concept. Validation of the assessment framework reveals a satisfying agreement to non-linear simulation results, showing an average root mean square deviation of only approximately eight percent in terms of total strain energy density. In order to study the effect of bulk and surface imperfections on the fatigue strength of cast steel components, defect-afflicted large-scale specimens are assessed by the presented elastic–plastic framework, yielding fatigue strength results which merge into the scatter band of the derived design limit curve. As the conducted fatigue assessment is based solely on linear–elastic two-dimensional simulations, the computational effort is substantially decreased. Within the present study, a reduction of approximately 400 times in computation time is observed. Hence, the established assessment framework presents an engineering-feasible method to evaluate the fatigue life of imperfective cast steel components based on rapid total strain energy density calculations.
Phycoremediation Processes for Secondary Effluent from Sewage Treatment Plants Using Photosynthetic Microorganisms: A Review
Authors: Pérez-Mora Lina Susana , Mejia-da-Silva Lauris del Carmen , Cezare-Gomes Eleane de Almeida , Santo Évellin do Espirito , Gohara-Beirigo Aline Kirie , Matsudo Marcelo Chuei , Nardin Bruno Monteiro , Sant’Anna Célia Leite , Carvalho João Carlos Monteiro de
Published: April 2023

Taking into account the worrying scenario of water scarcity, it is essential to enable more efficient technologies for wastewater treatment. Wastewater may be treated by conventional biological processes that remove pathogenic organisms, particulate and soluble organic compounds, and other components. However, secondary effluents from treatment plants may still contain toxic elements or high concentrations of inorganic nutrients (mainly nitrogen and phosphorus), which enable the growth of photosynthetic microorganisms in water bodies, resulting in eutrophication. In this context, cultivation of photosynthetic microorganisms in secondary wastewater from sewage treatment allows the removal of nutrients from such wastewater, reducing the possibility of eutrophication. Moreover, microalgal biomass, produced in this tertiary wastewater treatment, may be harvested by different methods with the potential for different applications, such as fertilizer and biofuel.
Trapped Ideal Bose Gas with a Few Heavy Impurities
Authors: Hryhorchak Orest , Pastukhov Volodymyr
Published: April 2023

In this article, we formulate a general scheme for the calculation of the thermodynamic properties of an ideal Bose gas with one or two immersed static impurities, when the bosonic particles are trapped in a harmonic potential with either a quasi-1D or quasi-2D configuration. The binding energy of a single impurity and the medium-induced Casimir-like forces between the two impurities are numerically calculated for a wide range of temperatures and boson–impurity interaction strengths.
Spectral Properties of Exact Polarobreathers in Semiclassical Systems
Authors: Archilla Juan F. R. , Bajārs Jānis
Published: April 2023

In this paper, we study the spectral properties of polarobreathers, that is, breathers carrying charge in a one-dimensional semiclassical model. We adapt recently developed numerical methods that preserve the charge probability at every step of time integration without using the Born–Oppenheimer approximation, which is the assumption that the electron is not at equilibrium with the atoms or ions. We develop an algorithm to obtain exact polarobreather solutions. The properties of polarobreathers, both stationary and moving ones, are deduced from the lattice and charge variable spectra in the frequency–momentum space. We consider an efficient approach to produce approximate polarobreathers with long lifespans. Their spectrum allows for the determination of the initial conditions and the necessary parameters to obtain numerically exact polarobreathers. The spectra of exact polarobreathers become extremely simple and easy to interpret. We also solve the problem that the charge frequency is not an observable, but the frequency of the charge probability certainly is an observable.
Revealing Silicon’s Delithiation Behaviour through Empirical Analysis of Galvanostatic Charge–Discharge Curves
Authors: Huld Frederik T. , Mæhlen Jan Petter , Keller Caroline , Lai Samson Y. , Eleri Obinna E. , Koposov Alexey Y. , Yu Zhixin , Lou Fengliu
Published: April 2023

The galvanostatic charge–discharge (GCD) behaviour of silicon (Si) is known to depend strongly on morphology, cycling conditions and electrochemical environment. One common method for analysing GCD curves is through differential capacity, but the data processing required necessarily degrades the results. Here we present a method of extracting empirical information from the delithiation step in GCD data for Si at C-rates above equilibrium conditions. We find that the function is able to quickly and accurately determine the best fit to historical half-cell data on amorphous Si nanowires and thin films, and analysis of the results reveals that the function is capable of distinguishing the capacity contributions from the Li3.5Si and Li2Si phases to the total capacity. The method can also pick up small differences in the phase behaviour of the different samples, making it a powerful technique for further analysis of Si data from the literature. The method was also used for predicting the size of the reservoir effect (the apparent amount of Li remaining in the electrode), making it a useful technique for quickly determining voltage slippage and related phenomena. This work is presented as a starting point for more in-depth empirical analysis of Si GCD data.
Finite Element Modeling of Residual Hearing after Cochlear Implant Surgery in Chinchillas
Authors: Castle Nicholas , Liang Junfeng , Smith Matthew , Petersen Brett , Matson Cayman , Eldridge Tara , Zhang Ke , Lee Chung-Hao , Liu Yingtao , Dai Chenkai
Published: April 2023

Cochlear implant (CI) surgery is one of the most utilized treatments for severe hearing loss. However, the effects of a successful scala tympani insertion on the mechanics of hearing are not yet fully understood. This paper presents a finite element (FE) model of the chinchilla inner ear for studying the interrelationship between the mechanical function and the insertion angle of a CI electrode. This FE model includes a three-chambered cochlea and full vestibular system, accomplished using µ-MRI and µ-CT scanning technologies. This model’s first application found minimal loss of residual hearing due to insertion angle after CI surgery, and this indicates that it is a reliable and helpful tool for future applications in CI design, surgical planning, and stimuli setup.
Nanomaterials for Skin Cancer Photoimmunotherapy
Authors: Relvas Carlota M. , Santos Susana G. , Oliveira Maria J. , Magalhães Fernão D. , Pinto Artur M.
Published: April 2023

Skin cancer is one of the most common types of cancer, and its incidence continues to increase. It is divided into two main categories, melanoma and non-melanoma. Treatments include surgery, radiation therapy, and chemotherapy. The relatively high mortality in melanoma and the existing recurrence rates, both for melanoma and non-melanoma, create the need for studying and developing new approaches for skin cancer management. Recent studies have focused on immunotherapy, photodynamic therapy, photothermal therapy, and photoimmunotherapy. Photoimmunotherapy has gained much attention due to its excellent potential outcomes. It combines the advantages of photodynamic and/or photothermal therapy with a systemic immune response, making it ideal for metastatic cancer. This review critically discusses different new nanomaterials’ properties and mechanisms of action for skin cancer photoimmunotherapy and the main results obtained in the field.
Onabotulinum Toxin A Intradetrusor Injections in Children with Neurogenic Lower Urinary Tract Dysfunction: Long-Term Histological Effects on the Bladder Wall
Authors: Pellegrino Chiara , Forlini Valentina , Lena Federica , Capitanucci Maria Luisa , Diomedi Camassei Francesca , Castelli Enrico , Mosiello Giovanni
Published: April 2023

Background: In the last twenty-five years, Onabotulinum Toxin A (BTX-A) has gained increasing popularity for neurogenic lower urinary tract dysfunction (NLUTD) treatment. To maintain its efficacy, repeated BTX-A intradetrusor injections are required over time, with unknown effects on the bladder wall in children. The aim of this paper is to report long-term effects on the bladder wall in children treated with BTX-A. Methods: Children with NLUTD not responsive to anticholinergics were treated with BTX-A, according to our protocol, with bladder wall control using endoscopic cold-cup biopsy. Specimens were evaluated considering edema, chronic inflammation, and fibrosis. Results: Of the 230 patients treated from 1997 to 2022, we considered only specimens obtained in patients who had received ≥5 treatments (36 children), considered as the threshold to evaluate clinical effectiveness on long-term treatment with BTX-A. Most of them had congenital NLUTD (25 patients) and detrusor overactivity (27 patients). In all, increased edema and chronic inflammation with reduced fibrosis over time was reported; these data were not statistically significant. No difference was observed between patients with congenital and acquired diseases. Conclusions: Repeated intradetrusor BTX-A injections are not related to significant histological alterations in children, similarly with adults, and repeated injections could be considered safe.
Novel Therapeutic Targets for the Treatment of Atopic Dermatitis
Authors: Tsuji Gaku , Yamamura Kazuhiko , Kawamura Koji , Kido-Nakahara Makiko , Ito Takamichi , Nakahara Takeshi
Published: April 2023

Atopic dermatitis (AD) is a chronic inflammatory skin disease that significantly impacts quality of life. The pathogenesis of AD is a complex combination of skin barrier dysfunction, type II immune response, and pruritus. Progress in the understanding of the immunological mechanisms of AD has led to the recognition of multiple novel therapeutic targets. For systemic therapy, new biologic agents that target IL-13, IL-22, IL-33, the IL-23/IL-17 axis, and OX40-OX40L are being developed. Binding of type II cytokines to their receptors activates Janus kinase (JAK) and its downstream signal, namely signal transduction and activator of transcription (STAT). JAK inhibitors block the activation of the JAK-STAT pathway, thereby blocking the signaling pathways mediated by type II cytokines. In addition to oral JAK inhibitors, histamine H4 receptor antagonists are under investigation as small-molecule compounds. For topical therapy, JAK inhibitors, aryl hydrocarbon receptor modulators, and phosphodiesterase-4 inhibitors are being approved. Microbiome modulation is also being examined for the treatment of AD. This review outlines current and future directions for novel therapies of AD that are currently being investigated in clinical trials, focusing on their mechanisms of action and efficacy. This supports the accumulation of data on advanced treatments for AD in the new era of precision medicine.
HIV-HBV Coinfection—Current Challenges for Virologic Monitoring
Authors: Ruta Simona , Grecu Laura , Iacob Diana , Cernescu Costin , Sultana Camelia
Published: April 2023

HIV-HBV coinfected patients have higher rates of liver-related morbidity, hospitalizations, and mortality compared to HBV or HIV mono-infected ones. Clinical studies have shown an accelerated progression of liver fibrosis and an increased incidence of HCC, resulting from the combined action of HBV replication, immune-mediated hepatocytolysis, and HIV-induced immunosuppression and immunosenescence. Antiviral therapy based on dually active antiretrovirals is highly efficient, but late initiation, global disparities in accessibility, suboptimal regimens, and adherence issues may limit its impact on the development of end-stage liver disease. In this paper, we review the mechanisms of liver injuries in HIV-HBV coinfected patients and the novel biomarkers that can be used for treatment monitoring in HIV-HBV coinfected persons: markers that assess viral suppression, markers for liver fibrosis evaluation, and predictors of oncogenesis.
The Effects of Antipsychotics in Experimental Models of Krabbe Disease
Authors: Sharma Kapil , Dev Kumlesh K.
Published: April 2023

The role of altered myelin in the onset and development of schizophrenia and changes in myelin due to antipsychotics remains unclear. Antipsychotics are D2 receptor antagonists, yet D2 receptor agonists increase oligodendrocyte progenitor numbers and limit oligodendrocyte injury. Conflicting studies suggest these drugs promote the differentiation of neural progenitors to oligodendrocyte lineage, while others report antipsychotics inhibit the proliferation and differentiation of oligodendrocyte precursors. Here, we utilised in-vitro (human astrocytes), ex-vivo (organotypic slice cultures) and in-vivo (twitcher mouse model) experimental study designs of psychosine-induced demyelination, a toxin that accumulates in Krabbe disease (KD), to investigate direct effects of antipsychotics on glial cell dysfunction and demyelination. Typical and atypical antipsychotics, and selective D2 and 5HT2A receptor antagonists, attenuated psychosine-induced cell viability, toxicity, and morphological aberrations in human astrocyte cultures. Haloperidol and clozapine reduced psychosine-induced demyelination in mouse organotypic cerebellar slices. These drugs also attenuated the effects of psychosine on astrocytes and microglia and restored non-phosphorylated neurofilament levels, indicating neuroprotective effects. In the demyelinating twitcher mouse model of KD, haloperidol improved mobility and significantly increased the survival of these animals. Overall, this study suggests that antipsychotics directly regulate glial cell dysfunction and exert a protective effect on myelin loss. This work also points toward the potential use of these pharmacological agents in KD.
Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem Cells
Authors: Mélou Caroline , Pellen-Mussi Pascal , Novello Solen , Brézulier Damien , Novella Agnès , Tricot Sylvie , Bellaud Pascale , Chauvel-Lebret Dominique
Published: April 2023

The objective of the present work was to develop a three-dimensional culture model to evaluate, in a short period of time, cartilage tissue engineering protocols. The spheroids were compared with the gold standard pellet culture. The dental mesenchymal stem cell lines were from pulp and periodontal ligament. The evaluation used RT-qPCR and Alcian Blue staining of the cartilage matrix. This study showed that the spheroid model allowed for obtaining greater fluctuations of the chondrogenesis markers than for the pellet one. The two cell lines, although originating from the same organ, led to different biological responses. Finally, biological changes were detectable for short periods of time. In summary, this work demonstrated that the spheroid model is a valuable tool for studying chondrogenesis and the mechanisms of osteoarthritis, and evaluating cartilage tissue engineering protocols.