bluuebunny/crossref_metadata_2025 huggingface dataset:
Table S2 from Discovery and Characterization of Brigimadlin, a Novel and Highly Potent MDM2–p53 Antagonist Suitable for Intermittent Dose Schedules
Authors: Gollner Andreas , Rudolph Dorothea , Weyer-Czernilofsky Ulrike , Baumgartinger Rosa , Jung Peter , Weinstabl Harald , Ramharter Jürgen , Grempler Rolf , Quant Jens , Rinnenthal Jörg , Pérez Pitarch Alejandro , Golubovic Bojana , Gerlach Daniel , Bader Gerd , Wetzel Kristiane , Otto Sebastian , Mandl Christian , Boehmelt Guido , McConnell Darryl B. , Kraut Norbert , Sini Patrizia
Published: December 2024

Antibodies used for Western blot analysis.
Table S12 from Discovery and Characterization of Brigimadlin, a Novel and Highly Potent MDM2–p53 Antagonist Suitable for Intermittent Dose Schedules
Authors: Gollner Andreas , Rudolph Dorothea , Weyer-Czernilofsky Ulrike , Baumgartinger Rosa , Jung Peter , Weinstabl Harald , Ramharter Jürgen , Grempler Rolf , Quant Jens , Rinnenthal Jörg , Pérez Pitarch Alejandro , Golubovic Bojana , Gerlach Daniel , Bader Gerd , Wetzel Kristiane , Otto Sebastian , Mandl Christian , Boehmelt Guido , McConnell Darryl B. , Kraut Norbert , Sini Patrizia
Published: December 2024

Preliminary human PK parameters of brigimadlin.
Supplementary Figure S3 from TR-107, an Agonist of Caseinolytic Peptidase Proteolytic Subunit, Disrupts Mitochondrial Metabolism and Inhibits the Growth of Human Colorectal Cancer Cells
Authors: Giarrizzo Michael , LaComb Joseph F. , Patel Hetvi R. , Reddy Rohan G. , Haley John D. , Graves Lee M. , Iwanowicz Edwin J. , Bialkowska Agnieszka B.
Published: December 2024

Supplementary Figure S3. Cell confluence images confirm a dose- and time-dependent inhibition of CRC cell proliferation. Colorectal cancer cells (A) LoVo, (B) NCI-H508, (C) LS 174T, and (D) RKO were treated in a 6-well plate format with vehicle control (0.1% DMSO) or indicated concentrations of TR-107 for 24, 48, and 72 hours. Phase-contrast live images (10× zoom) of cell confluence were captured at each time point. Images were processed with ImageJ and cell confluence was measured with PHANTAST 2.0 software as described in Materials and Methods. Data are presented as mean ± SD values (N=9). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Supplementary Table S1 from Fatty Acid Derivatization and Cyclization of the Immunomodulatory Peptide RP-182 Targeting CD206high Macrophages Improve Antitumor Activity
Authors: Singh Sitanshu S. , Calvo Raul , Kumari Anju , Sable Rushikesh V. , Fang Yuhong , Tao Dingyin , Hu Xin , Castle Sarah Gray , Nahar Saifun , Li Dandan , Major Emily , Sanchez Tino W. , Kato Rintaro , Xu Xin , Zhou Jian , Liu Liang , LeClair Christopher A. , Simeonov Anton , Baljinnyam Bolormaa , Henderson Mark J. , Marugan Juan , Rudloff Udo
Published: December 2024

Analytical data of investigated peptide analogues
Supplementary Table 1 from Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity
Authors: Rana Priyanka S. , Ignatz-Hoover James J. , Guo Chunna , Mosley Amber L. , Malek Ehsan , Federov Yuriy , Adams Drew J. , Driscoll James J.
Published: December 2024

Supplementary Table 1
Figure S8 from Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity
Authors: Rana Priyanka S. , Ignatz-Hoover James J. , Guo Chunna , Mosley Amber L. , Malek Ehsan , Federov Yuriy , Adams Drew J. , Driscoll James J.
Published: December 2024

Figure S8. Effect of in vivo administration of Compound A on myeloma xenotransplants in NSG mice. a. Schematic representation of the experimental details with a timeline of treatments and tumor measurements. b. Relative luciferase activity in MM1.S and MM1.S-Luc cells. c. Average TBW of mice from each treatment group. d. In vivo effect of Compound A on tumor volume of MM1.S-Luc cells in OT-1 mice. Shown is the average tumor volume (y-axis) vs. time from implantation (x-axis) for each treatment group. e. Tumor monitoring was performed using the IVIS spectrum imaging system where BLI was quantified as total flux (p/s) as a measure of total tumor burden (y-axis) relative to days post-implantation (x-axis). Total flux is a measure of the radiance (photons/sec) in each pixel summed or integrated over the ROI area (cm2) x 4π. Average radiance, a measure of luminescence, was calculated by summing the radiance from each pixel within the ROI and dividing it by the number of pixels or super pixels, resulting in units of photons/second/cm²/steradian.
Figure S7 from Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity
Authors: Rana Priyanka S. , Ignatz-Hoover James J. , Guo Chunna , Mosley Amber L. , Malek Ehsan , Federov Yuriy , Adams Drew J. , Driscoll James J.
Published: December 2024

Figure S7. Effect of Compound A pretreatment in vitro on the growth of E.G7-Ova-GFP-Luc tumors in vivo. a. Schematic representation of the experimental details with a timeline of treatments and tumor measurements. b. Phase contrast and FITC images of E.G7-Ova and E.G7-Ova-GFP-Luc cells. Scale bar = 100µm c. Relative luciferase activity in E.G7-Ova and E.G7-Ova-GFP-Luc cells. d. TBW for individual mice treated with either vehicle or Compound A. e. Average total flux as a measure of tumor burden in mice injected with vehicle or Compound A pretreated E.G7-Ova-Luc-GFP cells. Tumor burden (y-axis) was quantified as total flux (p/s) and monitored post-implantation (x-axis). Total flux represents a measure of the radiance (photons/sec) in each pixel integrated over the ROI area (cm2) x 4π. Average radiance was calculated by summing the radiance from each pixel within the ROI and then dividing by the number of pixels, resulting in units of photons/second/cm²/steradian. f. BLI of E.G7-Ova-GFP-Luc tumors from vehicle and Compound A-treated cells. Negative control represents an OT-1 mouse that was not injected with E.G7-Ova-GFP-Luc cells nor treated. Scale bar = 2 cm g. Percent survival of mice following injection of E.G7-Ova-GFP-Luc cells pretreated with either vehicle or Compound A.
Supplementary Table S1 from Separable Cell Cycle Arrest and Immune Response Elicited through Pharmacological CDK4/6 and MEK Inhibition in RASmut Disease Models
Authors: Wu Jin , Wang Jianxin , O’Connor Thomas N. , Tzetzo Stephanie L. , Gurova Katerina V. , Knudsen Erik S. , Witkiewicz Agnieszka K.
Published: December 2024

Supplementary Table S1. Oligonucleotide real-time qPCR primers used.
Supplemental Fig. S10 from Trametinib Potentiates Anti-PD-1 Efficacy in Tumors Established from Chemotherapy-Primed Pancreatic Cancer Cells
Authors: Pham Thao D. , Metropulos Anastasia E. , Mubin Nida , Becker Jeffrey H. , Shah Dhavan , Spaulding Christina , Shields Mario A. , Bentrem David J. , Munshi Hidayatullah G.
Published: December 2024

Supplemental Fig. S10. The combination of Trametinib and anti-PD-1 antibody attenuates the immunosuppressive TiME of the KPC2138-FIO tumors. A-B. Mice with established orthotopic 2138-FIO (A) and subcutaneous (B) tumors were randomized and treated with Trametinib (1 mg/kg, daily) or vehicle control in combination with an anti-PD-1 antibody (250 μg, three times/week) or a control isotype-matched IgG antibody (250 μg, three times/week). After 2 weeks, tumors were collected for analysis by flow cytometry for the frequency of all tumor-associated macrophages (TAM) (CD45+CD11b+F480+), M2 (CD45+CD11b+F480+Arg1+), and M1 TAMs (CD45+CD11b+F480+MHCII+). C. Subcutaneous KPC2138-FIO tumors at endpoint were also evaluated for the expression of select immunosuppressive markers (CD206, PD-L1, Arginase1 (Arg1)) on granulocytic myeloid-derived suppressive cells (G-MDSCs; CD45+CD11b+Ly6G+Ly6C-), monocytic myeloid-derived suppressive cells (M-MDSCs; CD45+CD11b+Ly6G-Ly6C+), and TAMs. One-way ANOVA, followed by Tukey multiple comparison test. *, p<0.05 **, p<0.01 ***, p<0.001 ns, non-significant.
Table S1 from Preclinical Characterization of ARX517, a Site-Specific Stable PSMA-Targeted Antibody–Drug Conjugate for the Treatment of Metastatic Castration-Resistant Prostate Cancer
Authors: Skidmore Lillian K. , Mills David , Kim Ji Young , Knudsen Nick A. , Nelson Jay D. , Pal Manoj , Wang Jianing , GC Kedar , Gray Michael J. , Barkho Wisam , Nagaraja Shastri Prathap , Ramprasad Mysore P. , Tian Feng , O’Connor Daniel , Buechler Ying J. , Zhang Shawn Shao-Hui
Published: December 2024

ARX517 is stable over 21-day incubation in human serum. ARX517 was incubated for the indicated times at 37C, 5% CO2 in pooled human serum. Time point samples were quantified in the ARX517 Total Ab (TA) and Intact ADC assay to assess stability.
Data from Discovery and Characterization of Brigimadlin, a Novel and Highly Potent MDM2–p53 Antagonist Suitable for Intermittent Dose Schedules
Authors: Gollner Andreas , Rudolph Dorothea , Weyer-Czernilofsky Ulrike , Baumgartinger Rosa , Jung Peter , Weinstabl Harald , Ramharter Jürgen , Grempler Rolf , Quant Jens , Rinnenthal Jörg , Pérez Pitarch Alejandro , Golubovic Bojana , Gerlach Daniel , Bader Gerd , Wetzel Kristiane , Otto Sebastian , Mandl Christian , Boehmelt Guido , McConnell Darryl B. , Kraut Norbert , Sini Patrizia
Published: December 2024

Abstract
p53 is known as the guardian of the genome and is one of the most important tumor suppressors. It is inactivated in most tumors, either via tumor protein p53 (TP53) gene mutation or copy number amplification of key negative regulators, e.g., mouse double minute 2 (MDM2). Compounds that bind to the MDM2 protein and disrupt its interaction with p53 restore p53 tumor suppressor activity, thereby promoting cell cycle arrest and apoptosis. Previous clinical experience with MDM2–p53 protein–protein interaction antagonists (MDM2–p53 antagonists) has demonstrated that thrombocytopenia and neutropenia represent on-target dose-limiting toxicities that might restrict their therapeutic utility. Dosing less frequently, while maintaining efficacious exposure, represents an approach to mitigate toxicity and improve the therapeutic window of MDM2–p53 antagonists. However, to achieve this, a molecule possessing excellent potency and ideal pharmacokinetic properties is required. Here, we present the discovery and characterization of brigimadlin (BI 907828), a novel, investigational spiro-oxindole MDM2–p53 antagonist. Brigimadlin exhibited high bioavailability and exposure, as well as dose-linear pharmacokinetics in preclinical models. Brigimadlin treatment restored p53 activity and led to apoptosis induction in preclinical models of TP53 wild-type, MDM2-amplified cancer. Oral administration of brigimadlin in an intermittent dosing schedule induced potent tumor growth inhibition in several TP53 wild-type, MDM2-amplified xenograft models. Exploratory clinical pharmacokinetic studies (NCT03449381) showed high systemic exposure and a long plasma elimination half-life in patients with cancer who received oral brigimadlin. These findings support the continued clinical evaluation of brigimadlin in patients with MDM2-amplified cancers, such as dedifferentiated liposarcoma.
Supplementary Figure-4 from TEAD2 Promotes Hepatocellular Carcinoma Development and Sorafenib Resistance via TAK1 Transcriptional Activation
Authors: Zhang Yahui , Ren Yidan , Dong Guoying , Jiao Qinlian , Guo Nan , Gao Ping , Li Ya , Wang Yunshan , Zhao Wei
Published: December 2024

Supplementary Figure-4
Supplementary Mat & Methods from Ruthenium Drug BOLD-100 Regulates BRAFMT Colorectal Cancer Cell Apoptosis through AhR/ROS/ATR Signaling Axis Modulation
Authors: Griffin Daryl , Carson Robbie , Moss Debbie , Sessler Tamas , Lavin Deborah , Tiwari Vijay K. , Karelia Shivaali , Kennedy Richard , Savage Kienan I. , McDade Simon , Carie Adam , Pankovich Jim , Bazett Mark , Van Schaeybroeck Sandra
Published: December 2024

Supplementary M & M
Figure 6 from Ruthenium Drug BOLD-100 Regulates BRAFMT Colorectal Cancer Cell Apoptosis through AhR/ROS/ATR Signaling Axis Modulation
Authors: Griffin Daryl , Carson Robbie , Moss Debbie , Sessler Tamas , Lavin Deborah , Tiwari Vijay K. , Karelia Shivaali , Kennedy Richard , Savage Kienan I. , McDade Simon , Carie Adam , Pankovich Jim , Bazett Mark , Van Schaeybroeck Sandra
Published: December 2024

BOLD-100 regulates apoptosis through BRAFMT-dependent AhR/CYP1A1/ROS/ATR axis activation. A, String network formed by the significant upregulated genes, 3 hours following BOLD-100 treatment in VACO432 cell line, form a cluster around AhR. B, VACO432 cells (left) were treated for indicated times with 50 μmol/L BOLD-100. CYP1A1, CYP1B1, TIPARP, LINC0051, and ALDH1A3 mRNA were quantified using RT-PCR. Raw values were normalized to the expression of housekeeping genes ACTB and GAPDH and were analyzed using the ΔΔCT method. mRNA levels presented are relative to SC. VACO432 and VT1 cells (right) were pretreated with 10 μmol/L CH-223191 (CH) for 3 hours and thereafter treated with 50 μmol/L BOLD-100 for 3 hours, and CYP1A1 (center) and TIPARP (right) mRNA was quantified using RT-PCR. CT, control. C, VACO432 cells were preincubated with vemurafenib (VEM) for 3 hours followed by BOLD-100 treatment for 24 hours and CYP1A1 and AhR mRNA was quantified using RT-PCR. D, VACO432 cells were pretreated with CH-223191 at indicated concentrations for 3 hours and thereafter treated with 50 μmol/L BOLD-100 for 24 hours and ROS levels assessed using the ROS-Glo H2O2 Assay Kit. E, Colorectal cancer cells were pretreated with 10 μmol/L CH-223191 (CH) for 3 hours and thereafter treated with 50 μmol/L BOLD-100 (B-100) for 24 hours and apoptosis assessed by WB for PARP (top) and Caspase 3/7 activity assay (bottom). Expression of pATRT1989, ATR and γH2AX was also determined. F, Schematic overview of proposed model. Oncogenic BRAF promotes BOLD-100 induced ATR/CHK1 activation by regulating replication stress through AhR/CYP1A1 and ROS. Coadministration of ATR inhibition (e.g., AZD6738) to BOLD-100 may promote replication fork collapse and apoptosis in BRAFMT colorectal cancer.
Figure 3 from Ruthenium Drug BOLD-100 Regulates BRAFMT Colorectal Cancer Cell Apoptosis through AhR/ROS/ATR Signaling Axis Modulation
Authors: Griffin Daryl , Carson Robbie , Moss Debbie , Sessler Tamas , Lavin Deborah , Tiwari Vijay K. , Karelia Shivaali , Kennedy Richard , Savage Kienan I. , McDade Simon , Carie Adam , Pankovich Jim , Bazett Mark , Van Schaeybroeck Sandra
Published: December 2024

BOLD-100 rewires the signaling network of BRAFMT colorectal cancer cells. RNA-seq of BOLD-100 treated VACO432 and VT1 cells for 3 and 24 hours was performed. A, PCA plot displaying the separation between the BRAFMT VACO432 and VT1 BRAFWT clone following treatment with 24 μmol/L BOLD-100 for 3 and 24 hours. B and C, Volcano plots show the up- and downregulated genes following BOLD-100 treatment at the indicated time-points in VACO432 cells. Dashed lines on the x and y-axis indicate log2ratio of 0.58/−0.58, and −log10P value = 1.3, respectively. KEGGS pathway analysis was performed using DEG (FC: +/− 1.5 P: < 0.05) genelists for VACO432 cells treated for 3 and 24 hours with BOLD-100. D, VACO432 cells (left) were co-treated with 50 μmol/L BOLD-100 alone or combined with a panel of 61 small molecule inhibitors for 48 hours and cell viability assessed using the CTG assay. Three concentrations per drug were tested (Supplementary Table S5). Waterfall plot showing mean (n = 3) robust Z-scores (rZ) for each compound concentration used in the drug screen. Negative rZ-scores indicate agents that are sensitive to BOLD-100, and vice versa. Dashed lines on graphs indicate values of 1.0 and −1.0. rZ-scores for 2/3 concentrations for AZD6738 are highlighted in red. Positive hit from primary screen (right). BRAFMT COLO-205 and HT-29 colorectal cancer cells were treated with BOLD-100 alone or combined with AZD6738 for 72 hours and cell viability assessed using the CTG assay. Absolute cell viability is shown. Dashed line indicates 50% and 25% cell viability. Representative of three independent experiments are shown. CONT, control.
Supplementary Table 7 from EHMT1/2 Inhibition Promotes Regression of Therapy-Resistant Ovarian Cancer Tumors in a CD8 T-cell–Dependent Manner
Authors: Nguyen Lily L. , Watson Zachary L. , Ortega Raquel , Woodruff Elizabeth R. , Jordan Kimberly R. , Iwanaga Ritsuko , Yamamoto Tomomi M. , Bailey Courtney A. , To Francis , Lin Shujian , Villagomez Fabian R. , Jeong Abigail D. , Guntupalli Saketh R. , Behbakht Kian , Gibaja Veronica , Arnoult Nausica , Chuong Edward B. , Bitler Benjamin G.
Published: December 2024

Hallmark pathways enriched in ID8-R cells treated with combinatory UNC0642/Olaparib compared to UNC0642 alone.
Supplementary Table 4 from EHMT1/2 Inhibition Promotes Regression of Therapy-Resistant Ovarian Cancer Tumors in a CD8 T-cell–Dependent Manner
Authors: Nguyen Lily L. , Watson Zachary L. , Ortega Raquel , Woodruff Elizabeth R. , Jordan Kimberly R. , Iwanaga Ritsuko , Yamamoto Tomomi M. , Bailey Courtney A. , To Francis , Lin Shujian , Villagomez Fabian R. , Jeong Abigail D. , Guntupalli Saketh R. , Behbakht Kian , Gibaja Veronica , Arnoult Nausica , Chuong Edward B. , Bitler Benjamin G.
Published: December 2024

Hallmark pathways negatively enriched in ID8-OR cells compared to ID8 cells
Supplementary Table 1 from Cannabidiol Enhances Atezolizumab Efficacy by Upregulating PD-L1 Expression via the cGAS–STING Pathway in Triple-Negative Breast Cancer Cells
Authors: Kim Bu Gyeom , Kim Bo Ram , Kim Dae Yeong , Kim Woo Young , Kang Sanghee , Lee Sun Il , Oh Sang Cheul
Published: December 2024

Antibodies for western blot analysis
Figure S5 from Integrated Germline and Somatic Features Reveal Divergent Immune Pathways Driving Response to Immune Checkpoint Blockade
Authors: Sears Timothy J. , Pagadala Meghana S. , Castro Andrea , Lee Ko-han , Kong JungHo , Tanaka Kairi , Lippman Scott M. , Zanetti Maurizio , Carter Hannah
Published: December 2024

Fig S5. (A-C). Measures of TIME infiltrates stratified by high vs low composite IC-Index scores. T-tests were used to compare means between groups. (D-E) Confusion matrices of somatic and germline IC-Indices (cutoff >=5) vs TIME score (cutoff=median).
Figure S3 from Integrated Germline and Somatic Features Reveal Divergent Immune Pathways Driving Response to Immune Checkpoint Blockade
Authors: Sears Timothy J. , Pagadala Meghana S. , Castro Andrea , Lee Ko-han , Kong JungHo , Tanaka Kairi , Lippman Scott M. , Zanetti Maurizio , Carter Hannah
Published: December 2024

Fig S3. A-C. SHAP derived nonlinear feature importance beeswarm plots of germline, somatic, and composite models. D-E. Correlation of immunogenicity features with TMB. F-G. Coefficients of features from linear regression-based germline and somatic models
Figure S1 from Integrated Germline and Somatic Features Reveal Divergent Immune Pathways Driving Response to Immune Checkpoint Blockade
Authors: Sears Timothy J. , Pagadala Meghana S. , Castro Andrea , Lee Ko-han , Kong JungHo , Tanaka Kairi , Lippman Scott M. , Zanetti Maurizio , Carter Hannah
Published: December 2024

Fig S1. A. eQTL-score schematic. SNPs alleles are oriented so that they are all in alignment in terms of direction of effects on gene expression. Number of alleles affecting gene expression are then summed into a continuous score B. Pearson correlation of Gene level eQTL-scores in TCGA and Discovery cohorts. P<=0.05 is indicated by an X.
Figure S4 from Inflammation Mediated by Gut Microbiome Alterations Promotes Lung Cancer Development and an Immunosuppressed Tumor Microenvironment
Authors: Rahal Zahraa , Liu Yuejiang , Peng Fuduan , Yang Sujuan , Jamal Mohamed A. , Sharma Manvi , Moreno Hannah , Damania Ashish V. , Wong Matthew C. , Ross Matthew C. , Sinjab Ansam , Zhou Tieling , Chen Minyue , Tarifa Reischle Inti , Feng Jiping , Chukwuocha Chidera , Tang Elizabeth , Abaya Camille , Lim Jamie K. , Leung Cheuk Hong , Lin Heather Y. , Deboever Nathaniel , Lee Jack J. , Sepesi Boris , Gibbons Don L. , Wargo Jennifer A. , Fujimoto Junya , Wang Linghua , Petrosino Joseph F. , Ajami Nadim J. , Jenq Robert R. , Moghaddam Seyed Javad , Cascone Tina , Hoffman Kristi , Kadara Humam
Published: December 2024

Supplementary Figure S4. Immune changes within the TME induced by inflammation related to gut microbiome alterations.
Table S6 from CD91 and Its Ligand gp96 Confer Cross-Priming Capabilities to Multiple APCs during Immune Responses to Nascent, Emerging Tumors
Authors: Nayak Devanshi A. , Sedlacek Abigail L. , Cillo Anthony R. , Watkins Simon C. , Binder Robert J.
Published: December 2024

DEGs- cDC1 vs Macrophages
Table S2 from CD91 and Its Ligand gp96 Confer Cross-Priming Capabilities to Multiple APCs during Immune Responses to Nascent, Emerging Tumors
Authors: Nayak Devanshi A. , Sedlacek Abigail L. , Cillo Anthony R. , Watkins Simon C. , Binder Robert J.
Published: December 2024

DEGs- cDC1 in CD91KO compared to WT.
Figure S7 from CD91 and Its Ligand gp96 Confer Cross-Priming Capabilities to Multiple APCs during Immune Responses to Nascent, Emerging Tumors
Authors: Nayak Devanshi A. , Sedlacek Abigail L. , Cillo Anthony R. , Watkins Simon C. , Binder Robert J.
Published: December 2024

Supplementary Figure 7. Co-incubation of gp96-EGFP+ donors APCs with CD45.1+ recipient APCs.
Table S3 from Peripheral Blood–Derived PD-1/CD28–CD19 CAR–Modified PD-1+ T-Cell Therapy in Patients with Solid Tumors
Authors: Zhang Zhen , Zhao Xuan , Zhao Qitai , Chen Xinfeng , Li Congcong , Liu Yaqing , Shen Chunyi , Song Lijie , Miao Lijun , Guo Fuyou , Mou Xiaoning , Zhao Jie , Gu Weiyue , Zhang Yi
Published: December 2024

Characteristics of patients with lung cancer who received anti-PD-1 antibody.
Table S1 from Peripheral Blood–Derived PD-1/CD28–CD19 CAR–Modified PD-1+ T-Cell Therapy in Patients with Solid Tumors
Authors: Zhang Zhen , Zhao Xuan , Zhao Qitai , Chen Xinfeng , Li Congcong , Liu Yaqing , Shen Chunyi , Song Lijie , Miao Lijun , Guo Fuyou , Mou Xiaoning , Zhao Jie , Gu Weiyue , Zhang Yi
Published: December 2024

The analysis of modified PD-1+ T cells for patients at each cycle.
Figure S9 from Peripheral Blood–Derived PD-1/CD28–CD19 CAR–Modified PD-1+ T-Cell Therapy in Patients with Solid Tumors
Authors: Zhang Zhen , Zhao Xuan , Zhao Qitai , Chen Xinfeng , Li Congcong , Liu Yaqing , Shen Chunyi , Song Lijie , Miao Lijun , Guo Fuyou , Mou Xiaoning , Zhao Jie , Gu Weiyue , Zhang Yi
Published: December 2024

Supplementary Figure S9. The level of serum cytokines in patients after modified PD-1+ T cell therapy.
Figure S12 from CD91 and Its Ligand gp96 Confer Cross-Priming Capabilities to Multiple APCs during Immune Responses to Nascent, Emerging Tumors
Authors: Nayak Devanshi A. , Sedlacek Abigail L. , Cillo Anthony R. , Watkins Simon C. , Binder Robert J.
Published: December 2024

Supplementary Figure 12. Tumor cells are not detectable in skin-draining lymph nodes of tumor-bearing mice.
Figure S4 from Peripheral Blood–Derived PD-1/CD28–CD19 CAR–Modified PD-1+ T-Cell Therapy in Patients with Solid Tumors
Authors: Zhang Zhen , Zhao Xuan , Zhao Qitai , Chen Xinfeng , Li Congcong , Liu Yaqing , Shen Chunyi , Song Lijie , Miao Lijun , Guo Fuyou , Mou Xiaoning , Zhao Jie , Gu Weiyue , Zhang Yi
Published: December 2024

Supplementary Figure S4. Validation of T cell clusters.
Figure S4 from PTPRZ1-Targeting RNA CAR T Cells Exert Antigen-Specific and Bystander Antitumor Activity in Glioblastoma
Authors: Martinez Bedoya Darel , Marinari Eliana , Davanture Suzel , Castillo Cantero Luis , Erraiss Sarah , Dockerill Millicent , Barluenga Sofia , Winssinger Nicolas , Schaller Karl , Bijlenga Philippe , Momjian Shahan , Voize Christel , Tissot Stéphanie R. , Kandalaft Lana E. , Hammel Philippe , Cosson Pierre , Walker Paul R. , Dutoit Valérie , Migliorini Denis
Published: December 2024

Bystander killing by anti-PTPRZ1 CAR-T cells using additional tumor cell lines and scFv
Figure S3 from PTPRZ1-Targeting RNA CAR T Cells Exert Antigen-Specific and Bystander Antitumor Activity in Glioblastoma
Authors: Martinez Bedoya Darel , Marinari Eliana , Davanture Suzel , Castillo Cantero Luis , Erraiss Sarah , Dockerill Millicent , Barluenga Sofia , Winssinger Nicolas , Schaller Karl , Bijlenga Philippe , Momjian Shahan , Voize Christel , Tissot Stéphanie R. , Kandalaft Lana E. , Hammel Philippe , Cosson Pierre , Walker Paul R. , Dutoit Valérie , Migliorini Denis
Published: December 2024

Bystander killing by 471_28z CAR-T cells depends on soluble mediators and does not affect macrophages
Data from Integrated Germline and Somatic Features Reveal Divergent Immune Pathways Driving Response to Immune Checkpoint Blockade
Authors: Sears Timothy J. , Pagadala Meghana S. , Castro Andrea , Lee Ko-han , Kong JungHo , Tanaka Kairi , Lippman Scott M. , Zanetti Maurizio , Carter Hannah
Published: December 2024

Abstract
Immune checkpoint blockade (ICB) has revolutionized cancer treatment; however, the mechanisms determining patient response remain poorly understood. Here, we used machine learning to predict ICB response from germline and somatic biomarkers and interpreted the learned model to uncover putative mechanisms driving superior outcomes. Patients with higher infiltration of T-follicular helper cells had responses even in the presence of defects in the MHC class-I (MHC-I). Further investigation uncovered different ICB responses in tumors when responses were reliant on MHC-I versus MHC-II neoantigens. Despite similar response rates, MHC II–reliant responses were associated with significantly longer durable clinical benefits (discovery: median overall survival of 63.6 vs. 34.5 months; P = 0.0074; validation: median overall survival of 37.5 vs. 33.1 months; P = 0.040). Characteristics of the tumor immune microenvironment reflected MHC neoantigen reliance, and analysis of immune checkpoints revealed LAG3 as a potential target in MHC II–reliant but not MHC I–reliant responses. This study highlights the value of interpretable machine learning models in elucidating the biological basis of therapy responses.
Data from Hyper-Interferon Sensitive Influenza Induces Adaptive Immune Responses and Overcomes Resistance to Anti–PD-1 in Murine Non–Small Cell Lung Cancer
Authors: Du Yushen , Salehi-Rad Ramin , Zhang Tian-hao , Crosson William P. , Abascal Jensen , Chen Dongdong , Shi Yuan , Jiang Hong , Tseng Yen-Wen , Ma Xi , Hong Mengying , Wang Sihan , Wang Xijuan , Tang Kejun , Hu Shiyao , Li Yuting , Ni Shaokai , Cai Yiqi , Tappuni Shahed , Shen Yong , Liu Bin , Sun Ren
Published: December 2024

Abstract
Despite recent advances in immunotherapy with immune checkpoint inhibitors, many patients with non–small cell lung cancer (NSCLC) fail to respond or develop resistance after an initial response. In situ vaccination (ISV) with engineered viruses has emerged as a promising antigen-agnostic strategy that can both condition the tumor microenvironment and augment antitumor T-cell responses to overcome immune resistance. We engineered a live attenuated viral vaccine, hyper–IFN-sensitive (HIS) virus, by conducting a genome-wide functional screening and introducing eight IFN-sensitive mutations in the influenza genome to enhance host IFN response. Compared with wild-type influenza, HIS replication was attenuated in immunocompetent hosts, enhancing its potential as a safe option for cancer therapy. HIS ISV elicited robust yet transient type I IFN responses in murine NSCLCs, leading to an enrichment of polyfunctional effector Th1 CD4+ T cells and cytotoxic CD8+ T cells into the tumor. HIS ISV demonstrated enhanced antitumor efficacy compared with wild-type in multiple syngeneic murine models of NSCLC with distinct driver mutations and varying mutational burden. This efficacy was dependent on host type 1 IFN responses and T lymphocytes. HIS ISV overcame resistance to anti–PD-1 in LKB1-deficient murine NSCLC, resulting in improved overall survival and systemic tumor-specific immunity. These studies provide compelling evidence to support further clinical evaluation of HIS as an “off-the-shelf” ISV strategy for patients with NSCLC refractory to immune checkpoint inhibitors.
ANCA-Associated Glomerulonephritis: Diagnosis and Therapy Proceedings of the Henry Shavelle Lectureship
Authors: Ivković Vanja , Windpessl Martin , Berke Ilay , Geetha Duvuru , Callemeyn Jasper , Norouzi Sayna , Bajema Ingeborg M. , Kronbichler Andreas
Published: December 2024

Background: Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) frequently affects the kidney. Glomerulonephritis (GN) in AAV, ANCA-GN, not only dictates therapeutic decisions but is also of relevance for overall survival influencing the risk of cardiovascular disease and serious infections. Summary: A diagnosis of ANCA-GN includes laboratory investigations including urinalysis and a thorough assessment of potential organ involvement. A kidney biopsy can be performed to ascertain the diagnosis but has an additional prognostic relevance and tools have been established to predict long-term kidney survival. Experimental biomarkers indicating kidney inflammation include urinary soluble CD163 and the presence of urinary T cells. Therapeutic options are refined and some of these therapies, such as the added value of performing plasma exchange, are the matter of controversial discussions. Safe reduction of cumulative exposure to glucocorticoids and eventually the use of avacopan to substantially reduce glucocorticoid exposure has been implemented in most centers. In the remission of maintenance, the optimal duration of therapy is still unclear, but extended use of rituximab as maintenance agent has shown long-term remission rates, thus limiting the damage accrued by relapsing disease and thus also reducing the risk of end-stage kidney disease (ESKD). Avacopan has been the first agent with a glomerular filtration rate-sparing effect, likely due to more rapid control of kidney inflammation. Those reaching ESKD should be evaluated for kidney transplantation and the risk of remaining on dialysis must be balanced against the risk of recurrence of disease following transplantation. Key Messages: The advent of a magnitude of landmark studies in ANCA-GN has refined diagnostic approaches, implemented tools to predict kidney outcome, and eventually led to the approval of newer therapies with avacopan, the latest addition to the armamentarium. Once ESKD is present, patients should be considered for kidney transplantation as remaining on dialysis portends poor overall prognosis.
Antecedentes do desempenho da função de servidores públicos: individuais, profissionais e organizacionais
Authors: Meurer Silvana Mannes , Ferreira Marília Paranaíba , Beuren Ilse Maria , Santos Vanderlei dos
Published: December 2024

This article examines antecedents of job performance of public servants, specifically individual (age, gender, resilience and work-related stress), professional (servant status and length of service) and organizational factors (the use of Management Information System - MIS and the understanding of internal processes through MIS). Data from the survey with 250 public servants from an institution responsible for upholding the State's legal order were analyzed using structural equation modeling. The results reveal that the individual factors age, gender, resilience and work-related stress influence the job performance of the public servants. The professional factor servant status was also found to have an impact, whereas length of service did not. Among the organizational factors, the use of MIS influences job performance, which contrasts with understanding of internal processes through MIS. These results suggest that the job performance of the public servants is more affected by individual factors than organizational ones. The length of service of the public servant was not significant, which suggests that experience does not necessarily reflect in performance. These findings provide important insights into the assignment of public servants for a given role and subsequent performance evaluation, in addition to filling a gap in the literature on practices in the public service.
Estudo Epidemiologico de febre typhoide em S. Paulo
Authors: Vieira F. Borges
Published: December 2024

O problema da febre typhoide em S. Paulo não é recente. Já de longa data, do tempo em que era conhecida dos nossos clinicos pelo nome de febres paulistas, vem ella ceifando vidas nesta cidade, com tal constancia que o povo como que já se habituou oom as suas visitas.
Valor da desinfecção na prophylaxia das doenças infectuosas
Authors: Vieira F. Borges
Published: December 2024

A theoria miasmatica, que attribuia a producção de doenças a emanações mephiticas provenientes de materia organica em putrefacção, aguas estagnadas e outras immundicies, prevaleceu no terreno empírico da sciencia, até que o genio de Pasteur viesse, com os seus memoraveis descobrimentos, revolucionar essa concepção. Era no meio que cerca o homem que se acreditava estar a origem da molestia. O descobrimento da existencia de seres microscopicos vivos e sua ubiquidade, sua presença sendo mormente verificada naquellas mesmas substancias organicas decompostas, ainda mais fortaleceram a crença na fonte extra-humana das doenças. Os minusculos organismos substituíram a noção dos miasmas, mas se originavam nos mesmos materiaes, que os causavam.
Os Fungos na Tuberculose Pulmonar
Authors: Gomes J. M. , Corrêa Clovis
Published: December 2024

The authors believe that pathologists do not pay enough attention to the fungi ohserved in lhe respiratory tract in a great number of morbid conditions and especially in cases of pulmonary tuherculosis. The action of these fungi should not be overlooked since they are able to produce grave diseases and even death. The opinion of several authors are cited in confirmation of this fact. Examination of the sputum - - Twenty-six samples of sputum of tuberculous individuais were examined; fungi were observed in 15 cases, hy the examination of a smear slide. Cultures - All samples of sputum were cultured for the isolation of fungi. Acid broth (2,0) was used, 2 hours at 37°C. followed by inoculation in Sabouraud's agar medium. Only 9 cases were negative. These organisms were then studied in reference to their determinative classification. The fungi were cultured on sugar media and no two similar organisms were observed. The classification proposed by Castellani, based on the fermentation of carbohydrates, seems insufficient excep in reference to a few fungi with fixed properties. Reports are given of experiments undertaken with guineapigs, inoculated in the lungs or peritoneum with triturated cultures of fungi. The lesions produced were studied macroscopic and microscopically. The amount of calcium present in the blood was studied on rabbits inoculated with fungi. The complement reaction was tried in the endeavor to differentiate a Monilia from an Ascomycete. lt was observed that sodium iodide exerted an inhibiting action in reference to the ill effects of pathogenic fungi. The clinicai syrnptoms of the mycoses were recorded.
The Image of Africa and Black Africans through the Translations of Heart of Darkness
Authors: Acar Alpaslan
Published: December 2024

The effects of translators’ personal lexical preferences on the perception of source texts are widely discussed descriptively, yet less researched experimentally. This article explores Joseph Conrad’s lexical choices and racist implications in presenting Africa and the fictional representation of black Africans. The study also questions whether Turkish translators manipulated the perception of Turkish expert readers through their lexical choices while presenting a fictional representation of black Africans to Turkish readers. To this end, 23 excerpts with racial overtones from the novella, Heart of Darkness, were examined and compared with corresponding excerpts from two translated Turkish versions of Heart of Darkness. Text-specific excerpts related to the representation of Africa and that of the African natives were selected. To avoid researcher bias, the judgements of seven experts in English Literature and/or Translation Studies were gained in the selection and analysis of the excerpts. The findings were analysed quantitatively and qualitatively. The results showed that the different translation strategies used by each of translators significantly affected the perception of the target readership. Also, it was found that translators are not passive receptors of source texts but rather serve as game-changers in manipulating the effect of the source text on the target readership in the case of racial overtones.
Scholarship with a Purpose: Frontiers of Gender Equality Pushes the Boundaries for Students and Scholars Alike
Authors: Petersen Carole J. , Atanasio Kate
Published: December 2024

This review article critiques Frontiers of Gender Equality: Transnational Legal Perspectives, edited by Rebecca J Cook. Composed of chapters centred on advancing gender equality, Frontiers differs from the typical edited collection because the authors worked collaboratively and shared a common purpose. They are all strongly committed to substantive equality (as opposed to formal equality) and recognise the importance of addressing intersectional discrimination. In their respective chapters the authors expand the discourse surrounding gender equality law by envisioning transformative approaches to righting gendered wrongs in domestic, regional and international human rights systems. While the collection is certainly valuable for scholars and legal practitioners, this review article considers its value for a different audience – that of graduate students in law and gender studies. Because the book is far more structured and unified than the typical edited collection, it could serve as a foundational textbook for an advanced comparative course on gender equality and the law. This review article – authored by a professor and a law student – analyses the book’s considerable strengths as a textbook. However, it also identifies certain weaknesses and suggests supplementary materials, in order to provide better coverage of the Asia-Pacific region.
City of Traders: Urbanization, Social Change, and Territorial Control in Medieval Fardowsa (Central Somaliland)
Authors: de Torres Rodríguez Jorge , González-Ruibal Alfredo , Fernández Manuel Antonio Franco , de la Torre García Adrián , Yravedra Sainz de los Terreros José , Lesur Josephine
Published: December 2024

This paper presents the results of the research conducted at the site of Fardowsa, a medieval town located in a strategic trade route in Central Somaliland. The excavations and surveys conducted at the site have confirmed the role of Fardowsa as a trading center between the 14th and 16th centuries, and have revealed the existence of privileged households within the site, with differential access to imports and livestock. The combination of data collected in Fardowsa makes this site the best-studied town in Somaliland for this period, and provides key information to help understand the process of emergence, development and abandonment of permanent settlements in this region of the Horn of Africa.
The Influence of the International Right to Adequate Housing in Ireland and Spain
Authors: Nic Shuibhne Emma N. , Quintiá-Pastrana Andrei
Published: December 2024

This article investigates and compares the influence of the international right to housing in Ireland and Spain, through the use of the functional comparative method. As part of this comparison, we created a set of indicators that aimed to identify evidence of the direct and indirect influence of the international right to housing on actions carried out at the national level to protect against evictions. In applying this method, we focus on three branches of state (the legislative, executive and judiciary) and civil society actors. Our analysis identifies instances of direct and indirect influence on actors in both countries and compares these results. We conclude that evidence of indirect influence is often more common than evidence of direct influence. We suggest this indirect influence may illustrate the role that the international right to housing may play behind the scenes in creating domestic eviction protections.
Editing and Printing Hadith Literature in Nineteenth-Century India: Aḥmad ʿAlī Sahāranpūrī and the Maṭbaʿ-i Aḥmadī Press
Authors: Zaman Muntasir
Published: December 2024

From manuscripts to various types of printing, the production of written knowledge has undergone drastic changes since the nineteenth century. What impact did this transitionary period have on hadith manuscripts, which enjoyed a complex textual tradition spanning centuries? Through a case study of the North Indian hadith scholar Aḥmad ʿAlī Sahāranpūrī (d. 1880) and his printing press, Maṭbaʿ-i Aḥmadī, this article contributes to the burgeoning scholarship on early-modern editorial practices. Focusing on the use of lithographic printing in nineteenth-century India, this study examines how Sahāranpūrī employed editorial techniques to print hadith literature that paralleled other prolific printing hubs like Cairo while also preserving the distinctive premodern characteristics of these texts. Moreover, this article will showcase the broader editorial trends in India at the time vis-à-vis hadith literature with a focus on what set Sahāranpūrī’s work apart from that of his fellow Indian editors.
Deleuze, Guattari, Khôra, and the Taiji Diagram
Authors: Zhang Peter
Published: December 2024

French philosopher, Gilles Deleuze, interprets khôra as a screen that has cosmological, genealogical, and psychological significances. Khôra is the womb of all that comes to be and becomes, including yin and yang, which are two modes of the life impulse and the two conspicuous elements of the Taiji Diagram. The ‘S’ line between yin and yang is a khôra or a sieve that ensures intercommunication and counter-dependence between yin and yang and that keeps the system dynamic, entropy-defying, playful, and vital. Deleuze’s interpretation of the Bergsonian notion of differentiation reveals the rationale of the Taiji Diagram, even if it is not his intention to do so. The outer boundary of the Taiji Diagram is the originary khôra that mediates between chaos and cosmos and that gives impetus to and sustains the process of chaosmosis, which has both cosmological and psychological implications. For Félix Guattari, chaosmosis indicates the process of subjectivation. The interruption of the process hinders the singularization and renewal of subjectivity and impairs psychological health. Therein lies the gist of Guattari’s book, Chaosmosis. The article belongs to the growing literature on khôralogy (a.k.a. chorology) and interology.
Redescription of Acartia (Acanthacartia) italica Steuer, 1910 (Copepoda, Calanoida), and description of the developmental stages from the small saline Lake Rogoznica, Adriatic Sea
Authors: KRŠINIĆ FRANO , BOXSHALL GEOFF A.
Published: December 2024

In Lake Rogoznica, a small saline and eutrophic lake on the coast of the Adriatic Sea, the copepod Acartia (Acanthacartia) italica Steuer, 1910 is common, occasionally as an extremely dense population. This phenomenon provided an opportunity for a redescription of the adults and for description of the developmental stages. The segmentation and setation patterns of the antennules, antennae and mandibles of A. italica are analysed in detail through the naupliar and copepodid phases and the other limbs are analysed through the copepodid phase. In addition, wider comparisons are made with available data for other species of the subgenus Acanthacartia Steuer, 1915.
A new species of Dropephylla Mulsant & Rey, 1880 from northeastern Türkiye, and faunistic records of some species of Omaliini from Western Palaearctic Region (Coleoptera: Staphylinidae: Omaliinae)
Authors: SHAVRIN ALEXEY V.
Published: December 2024

A new species of the genus Dropephylla Mulsant & Rey, 1880 is described and illustrated: D. loebli sp. nov. (northeastern Türkiye, Kars). Besides that, new faunistic data for 16 species (Omaliini) from the Western Palaearctic Region are provided. The aedeagus of D. cretica Jászay & Hlaváč, 2006 is illustrated. Several species are recorded from certain regions for the first time: D. caucasica (Kolenati, 1846) from Georgia, D. cretica from Türkiye and Lebanon, D. ioptera (Stephens, 1834) from Serbia, Phloeonomus (Phloeonomodes) minimus Erichson, 1839 from Ukraine, southwestern part of Russia and Türkiye, and Phyllodrepa floralis (Paykull, 1789) from Syria.
Girawa (Vernonia amygdalina) as Supplemental Feed and Its Effects on Weight Gain and Carcass Parameter of Somali Goats Fed Khat (Catha edulis) Leftover
Authors: Adem Kedir
Published: December 2024

The experiment was conducted to determine body weight change, Average daily gain, carcass parameter and Analysis of Partial Budget of Somali goat fed khat leftover, supplemented with graded levels of dried vernonia amygdalina leaf. For this experiment, 24 yearling male goats with a mean initial body weight of 12.57± 0.59 kg (means ± SD) were used. The experimental goats were blocked into six blocks of four animals in randomized complete block design depending on initial body weight. The four treatments were assigned randomly to each goat within a block. The experimental goats were accessed a basal diet of khat leftover and supplemented with 100 (T1), 200 (T2), 300 (T3) and 400g (T4) Vernonia amygdalina leaf. The experiment was undertaken for Ninety days of feeding trial followed by carcass evaluation at the end of the experiment. The results of this study showed that level of supplementation significantly (p < 0.05) improved average daily weight gain, feed conversion efficiency and had better carcass characteristics. Hot carcass weight (HCW; 5 (T1), 6 (T2), 7 (T3) and 8.25 kg (T4) and dressing percentage on empty body weight basis; 43.29 (T1), 44.48 (T2), 50.34 (T3) and 55.18 (T4) was higher (p < 0.05) for T4 compared to T1, T2 and T3. The lower level of supplementation recorded loss of 34 ETB/goat. Goats with higher level of supplementation (T4) had the highest net income (1626 ETB) as well as the highest marginal ret of (4.1 ETB).
Evaluation of Alley-cropping Agroforestry System and Conventional System of Agriculture on the Productivity of Cabbage (Brassica oleracea L.) in Dschang, West Region of Cameroon
Authors: Essomo Etanke , Arrey Besem , Honore Beyegue , Afanga Yannick , Inès Nyayi , Antoine Egoumé
Published: December 2024

Cabbage is one of the main vegetable crops grown in Cameroon. However, production is limited by rising prices and the scarcity of chemical fertilisers on the market. The rise in cost of fertilizers leaves some farmers reluctant to use fertilisers and as a result leads to lower yields, hence, low crop profitability. The aim of this study was to compare the two systems of agriculture; agroforestry system with the conventional system on the growth of cabbage (Brassica oleracea L.). Research plots were established on the campus of the University of Dschang, in the West Region of Cameroon. The comparisons were made on the basis of productivity, pest abundance and economic profitability. Data on yield and pest susceptibility variables were collected during the field trials. It was realised that the slug (Deroceras reticulatum) population was higher in the agroforestry system (4.62±2.41 slugs) than in the conventional system (1.03±0.55 slugs). The aphid population (Brevicoryne brassicae) was higher in the conventional cropping system (25.31±38.94 aphids) compared with the alley-cropping agroforestry system (0.71±0.62 aphids). Productivity variables showed that cabbage yield did not vary between the two production methods. It was 66.08 t/ha and 66.76 t/ha respectively for the agroforestry system and the conventional system. The economic analysis showed that the agroforestry system was more profitable than the conventional system. Thus, growing cabbage in an agroforestry system is an ecological and profitable strategy for agriculture that is more resilient to climatic variations.