bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential Use of COVID-19 Surgical Masks and Polyethylene Plastics in Developing Sustainable Concrete
Authors: Paul Suvash Chandra , Santo Md. Ahosun Habib , Nahid Sowmik Ahmed , Majumder Asifur Rahman , Al Mamun Md. Fahim , Basit Md Abdul , Babafemi Adewumi John
Published: September 2023

Managing disposable waste surgical face masks and plastic made from polyethylene (PE) resin is a real challenge. Thus, these are considered a great threat to the environment. Generally, surgical face masks are made of microplastic made of polypropylene materials. Both polypropylene and PE are not easily decomposable in the soil. Consequently, the presence of these waste materials can have detrimental effects on terrestrial and aquatic ecosystems, exacerbating the ongoing crisis faced by the animal kingdom and the broader biosphere. Hence, it is imperative to identify alternate and efficient methods for waste management. Given its significant economic importance, the construction industry holds a prominent position among many industries globally. Consequently, waste masks within the construction sector might assume a crucial role in mitigating plastic pollution. Concrete, one of the most widely used construction materials, is being adapted with various waste materials as the partial or complete substitutes for natural constituents, such as cement and aggregates. This study focused on using different percentages of used COVID-19 surgical masks in fiber form and PE as partial replacements of natural coarse aggregates in producing sustainable concrete. Mask fibers were used in concrete production at percentages of 0%, 0.5%, 1%, 1.5%, and 2% of the total volume of concrete. Similarly, PE aggregates replaced the coarse aggregates by volume at 0%, 5%, 10%, and 15% in concrete. The results showed that the strength of concrete reduced as the percentages of mask fiber and PE aggregates increased. However, the strength and crack-bridging capability of mask concrete are still acceptable for some structural and non-structural applications. The results obtained from this research could also help engineers to design sustainable concrete materials with mask fibers.
Comparison between Sugammadex and Neostigmine after Video-Assisted Thoracoscopic Surgery–Thymectomy in Patients with Myasthenia Gravis: A Single-Center Retrospective Exploratory Analysis
Authors: No Hyun-Joung , Yoo Young Chul , Oh Young Jun , Lee Hye Sun , Jeon Soyoung , Kweon Ki Hong , Kim Na Young
Published: September 2023

This single-center retrospective exploratory analysis evaluated the effects of sugammadex compared with neostigmine on postoperative recovery in patients with myasthenia gravis (MG) who underwent video-assisted thoracoscopic surgery (VATS)–thymectomy. This retrospective study included 180 patients with MG, aged >18 years, who received sugammadex (sugammadex group, n = 83) or neostigmine–glycopyrrolate (neostigmine group, n = 88) after VATS–thymectomy between November 2007 and December 2020. Inverse probability of treatment weighting (IPTW) adjustment was performed to balance the baseline characteristics between the two groups. The primary outcome was the length of postoperative hospital stay, and the secondary outcomes were the incidence of postoperative mortality and complications, as well as the postoperative extubation and reintubation rates, in the operating room after VATS–thymectomy; the outcomes were compared between the two groups. After IPTW adjustment, the sugammadex group showed a significantly shorter median postoperative hospital stay than the neostigmine group (4 (2, 4) vs. 5 (3, 6) days, respectively; p = 0.003). There were no significant differences between the two groups in the incidences of postoperative complications (including postoperative myasthenic crisis, nerve palsy, atelectasis, and pleural effusion). Patients with MG following VATS–thymectomy who received sugammadex showed a significantly shorter postoperative hospital stay than those who received neostigmine.
Bone Cement Implantation Syndrome: A Rare Disaster Following Cemented Hip Arthroplasties—Clinical Considerations Supported by Case Studies
Authors: Moldovan Flaviu
Published: September 2023

Severe symptoms such as hypoxemia, hypotension, and unexpected loss of consciousness may develop during surgical interventions that use polymethyl methacrylate (PMMA), or as it is commonly known, bone cement. Physicians recognize this amalgam of clinical manifestations more and more as a distinct entity that bears the name of bone cement implantation syndrome (BCIS). Trauma cases, especially hip fractures, are seen to have a higher incidence of developing this complication compared to orthopedic elective ones. This research aims to present a detailed description of six severe BCIS cases in order to raise awareness and to emphasize its importance. Five of them had fatal outcomes, which demonstrate the necessity of future research on this topic, as little is known about it presently. In the Discussion section, a narrative overview from the scientific literature is performed on potential risk factors, prevention measures, and management strategies. The experience gathered through this case series may aid medical staff in the development of diagnostic and therapeutic protocols, thus improving safety when cemented surgical techniques are used on a high-risk group of patients.
Parcellation-Based Connectivity Model of the Judgement Core
Authors: Hormovas Jorge , Dadario Nicholas B. , Tang Si Jie , Nicholas Peter , Dhanaraj Vukshitha , Young Isabella , Doyen Stephane , Sughrue Michael E.
Published: September 2023

Judgement is a higher-order brain function utilized in the evaluation process of problem solving. However, heterogeneity in the task methodology based on the many definitions of judgement and its expansive and nuanced applications have prevented the identification of a unified cortical model at a level of granularity necessary for clinical translation. Forty-six task-based fMRI studies were used to generate activation-likelihood estimations (ALE) across moral, social, risky, and interpersonal judgement paradigms. Cortical parcellations overlapping these ALEs were used to delineate patterns in neurocognitive network engagement for the four judgement tasks. Moral judgement involved the bilateral superior frontal gyri, right temporal gyri, and left parietal lobe. Social judgement demonstrated a left-dominant frontoparietal network with engagement of right-sided temporal limbic regions. Moral and social judgement tasks evoked mutual engagement of the bilateral DMN. Both interpersonal and risk judgement were shown to involve a right-sided frontoparietal network with accompanying engagement of the left insular cortex, converging at the right-sided CEN. Cortical activation in normophysiological judgement function followed two separable patterns involving the large-scale neurocognitive networks. Specifically, the DMN was found to subserve judgement centered around social inferences and moral cognition, while the CEN subserved tasks involving probabilistic reasoning, risk estimation, and strategic contemplation.
Our Experience with Hydrogel-Coated Trisacryl Microspheres in Uterine Artery Embolization for the Treatment of Symptomatic Uterine Fibroids and Adenomyosis: A Follow-Up of 11 Years
Authors: Tsikouras Panagiotis , Oikonomou Efthymios , Tsatsaris George , Bothou Anastasia , Kyriakou Dimitrios , Nikolettos Konstantinos , Nalmbanti Theopi , Peitsidis Panagiotis , Trypsanis Grigorios , Iatrakis George , Nikolettos Nikolaos , Souftas Vasileios
Published: September 2023

Uterine artery embolization (UAE) for the treatment of symptomatic uterine fibroids and non-controllable adenomyosis symptoms is a relatively new procedure for organ-preserving therapy. These benign conditions can become symptomatic in about 30% of women between the ages of 35 and 50. The purpose of the UAE either for fibroids or adenomyosis is the elimination of blood loss, the reduction in pain, and bulky or rectal pressure symptoms. The purpose of this study is to present our experience in UAE with the use of hydrogel-coated tris acryl microspheres for the treatment of symptomatic uterine fibroids and adenomyosis.
Key Area Recognition and Evaluation of Audio-Visual Landscape for Global Geoparks: A Case Study of Koktokay in China
Authors: Zhu Yiting , Pang Xueru , Zhou Chunshan
Published: September 2023

A comprehensive and scientific recognition and evaluation of landscape resources is an important prerequisite for the sustainable development of global geoparks, but the existing research lacks specific means and methods. In the case of the Koktokay Global Geopark (GGp), for example, in this study, we used GIS spatial analysis, SBE, and a questionnaire survey method to construct a comprehensive evaluation path and method for an audio-visual landscape for global geoparks and further built an audio-visual preference matrix. The research results show the following: (1) The Shenzhong Canyon scenic area has the best visual evaluation effect, whereas the Golden Triangle has the worst evaluation effect. (2) Tourists are generally satisfied with the soundscapes of the ten scenic locations in the Koktokay GGp. In addition, tourists do not think that a higher or lower volume of a soundscape would make their experience more comfortable or pleasant, so an increase in the threshold value of the sound level to a level that tourists can bear while traveling is possible. (3) The Shenzhong Canyon area is located in the key landscape area; the Aiguzi Mine and Cocosuri are located in the landscape natural development area; the No. 3 Mine Pit, Eremu Lakes, the Golden Triangle, and the Karadrola Falls are located in the landscape subpriority improvement area; and the Waterfall Fossil, Betula Forest, and Karashanger Earthquake Fault Zone are located in the landscape priority improvement area. The comprehensive audio-visual landscape evaluation method constructed in this study provides a methodological tool for evaluating other similar landscapes and provides professional guidance for the protection and development of geoparks.
A Special (Question) View on Wh-Doubling in Lombard Varieties
Authors: Garzonio Jacopo , Castro Enrico , Messina Jessica Rita
Published: September 2023

In this article, we examine a specific type of Wh-Doubling (WhD) that can be observed in some Northern Lombard varieties. Differently from all the previous types of WhD described for Lombard and other Northern Italo-Romance varieties, in the phenomenon we analyze, the two wh-forms are identical and display the -ɛ morpheme. Furthermore, in all the varieties where this type of WhD is present, it is associated with the encoding of special questions and cannot be used in true requests for new information. We propose an analysis of this construction assuming a split-CP representation where the higher wh-form activates a functional projection involved in the expression of special questions. We also hypothesize that this construction derives from an interrogative cleft; if this hypothesis is correct, the formation of the -ɛ morpheme and the re-analysis of the cleft as monoclausal are the two sides of the same process.
Acoustic Variability of /ptk/ and /bdɡ/ in Spanish: A Pilot Study
Authors: Butera Brianna
Published: September 2023

Propelled by existing research on stop consonant variability in Spanish, this pilot study provides a preliminary acoustic analysis of stop consonant lenition exhibited by speakers of six different varieties of Spanish in Latin America and Spain to explore the gradient acoustic variability in the production of /ptk/ and /bdɡ/ among speakers of different Spanish varieties. Using the acoustic correlate of relative intensity, this analysis considers the effect of various linguistic factors (phoneme, lexical stress, point of articulation, sonority) as well as the extralinguistic factor of Spanish variety on the production of stop consonants in initial, intervocalic position. Results display a higher degree of weakening among speakers of Peninsular and Insular varieties of Spanish compared with those of Latin-American varieties such as Colombian, Mexican, and Peruvian. These exploratory data support the gradient nature of consonant lenition and provide a baseline for future research on stop consonant variability across the Spanish-speaking world.
Mitochondrial DNA Copy Number Drives the Penetrance of Acute Intermittent Porphyria
Authors: Di Pierro Elena , Perrone Miriana , Franco Milena , Granata Francesca , Duca Lorena , Lattuada Debora , De Luca Giacomo , Graziadei Giovanna
Published: September 2023

No published study has investigated the mitochondrial count in patients suffering from acute intermittent porphyria (AIP). In order to determine whether mitochondrial content can influence the pathogenesis of porphyria, we measured the mitochondrial DNA (mtDNA) copy number in the peripheral blood cells of 34 patients and 37 healthy individuals. We found that all AIP patients had a low number of mitochondria, likely as a result of a protective mechanism against an inherited heme synthesis deficiency. Furthermore, we identified a close correlation between disease penetrance and decreases in the mitochondrial content and serum levels of PERM1, a marker of mitochondrial biogenesis. In a healthy individual, mitochondrial count is usually modulated to fit its ability to respond to various environmental stressors and bioenergetic demands. In AIP patients, coincidentally, the phenotype only manifests in response to endogenous and exogenous triggers factors. Therefore, these new findings suggest that a deficiency in mitochondrial proliferation could affect the individual responsiveness to stimuli, providing a new explanation for the variability in the clinical manifestations of porphyria. However, the metabolic and/or genetic factors responsible for this impairment remain to be identified. In conclusion, both mtDNA copy number per cell and mitochondrial biogenesis seem to play a role in either inhibiting or promoting disease expression. They could serve as two novel biomarkers for porphyria.
Comparison of CrN Coatings Prepared Using High-Power Impulse Magnetron Sputtering and Direct Current Magnetron Sputtering
Authors: Bai Heda , Li Jin , Gao Jialai , Ni Jinyang , Bai Yaxiong , Jian Jie , Zhao Lin , Bai Bowen , Cai Zeyun , He Jianchao , Chen Hongsheng , Leng Xuesong , Liu Xiangli
Published: September 2023

Chromium Nitride (CrN) coatings have widespread utilization across numerous industrial applications, primarily attributed to their excellent properties. Among the different methods for CrN coating synthesis, direct current magnetron sputtering (DCMS) has been the dominant technique applied. Nonetheless, with the expanded applications of CrN coatings, the need for enhanced mechanical performance is concurrently escalating. High-power impulse magnetron sputtering (HiPIMS), an innovative coating deposition approach developed over the past three decades, is gaining recognition for its capability of yielding coatings with superior mechanical attributes, thereby drawing significant research interest. Considering that the mechanical performance of a coating is fundamentally governed by its microstructural properties, a comprehensive review of CrN coatings fabricated through both techniques is presented. This review of recent literature aims to embark on an insightful comparison between DCMS and HiPIMS, followed by an examination of the microstructure of CrN coatings fabricated via both techniques. Furthermore, the exploration of the underlying factors contributing to the disparities in mechanical properties observed in CrN coatings is revealed. An assessment of the advantages and potential shortcomings of HiPIMS is discussed, offering insight into CrN coating fabrication.
Production of Biodegradable Polymeric Composites with the Addition of Waste
Authors: da Silva Fernandes Fernando Antonio , Serra Juan Carlos Valdés , de Oliveira Costa Dayriane do Socorro , Martin Camilo Andrés Guerrero
Published: September 2023

Several solutions have been presented to minimize the environmental impact generated by polymers produced from petroleum resources. This work produced a biopolymer using glycerol, starch (<5) and macaúba epicarp fiber (10–15–20–25–30%) as reinforcement. The interaction of glycerol with starch was favored by the addition of acetic acid (CH3COOH). The pH was adjusted with sodium hydroxide (NaOH) at a concentration of 0.1 mol·L−1. The characterization was carried out through scanning electron microscopy (SEM), infrared reflectance—FTIR, water solubility, biodegradability and technological properties. Through the results obtained in this work, it is observed that the tensile strength and modulus of elasticity are influenced by the addition of the fiber concentration; the sample that received a 30% addition presented 19.17 MPa and 348.12 MPa, respectively. All samples showed low solubility in water and low density, in addition to a high rate of degradability in soil with mass loss corresponding to 59% over a period of three months. The results of this investigation are satisfactory for the production of materials that can be used in everyday life, replacing conventional plastic.
The Influence of Poultry Manure-Derived Biochar and Compost on Soil Properties and Plant Biomass Growth
Authors: Dróżdż Danuta , Malińska Krystyna , Wystalska Katarzyna , Meers Erik , Robles-Aguilar Ana
Published: September 2023

Promising methods for managing poultry manure (PM) include converting poultry manure through pyrolysis to biochar, which can be used for soil applications. The overall goal of this study was to determine the effects of poultry manure-derived biochar and compost on the soil and growth of cherry tomatoes. The biochar obtained at 475 °C was characterized by a relatively high organic matter content of 39.47% and nitrogen content of 3.73%, while it had the lowest C/N ratio of 8.18. According to the recommendations of the EBC, the biochar obtained at 475 °C demonstrated the most beneficial effects in terms of fertilizing potential. The composting of poultry manure with the straw was successful, and the limit of 60 °C was exceeded, which allowed for the hygienization of the compost. The produced compost and biochar are sanitary safe and do not exceed the limits of heavy metal content. The lowest plant biomass was obtained from growing medium A with 3.6 g wet weight (0.24 g dry weight). The measurements of the height of cherry tomatoes showed that growing media D, E, and F allowed the plants to obtain from 602 to 654 mm in height.
Temperature and E-Poling Evolution of Structural, Vibrational, Dielectric, and Ferroelectric Properties of Ba1−xSrxTiO3 Ceramics (x = 0, 0.1, 0.2, 0.3, 0.4 and 0.45)
Authors: Suchanicz Jan , Sitko Dorota , Stanuch Krzysztof , Świerczek Konrad , Jagło Grzegorz , Kruk Andrzej , Kluczewska-Chmielarz Kamila , Konieczny Krzysztof , Czaja Piotr , Aleksandrowicz Jakub , Wieczorek Wojciech , Grygierek Justyna , Sokolowski Mariusz , Stachowski Grzegorz , Antonova Maija , Sternberg Andris
Published: September 2023

Lead-free Ba1−xSrxTiO3 (BST) (x = 0, 0.1, 0.2, 0.3, 0.4 and 0.45) ceramics were successfully prepared via the solid-state reaction route. A pure perovskite crystalline structure was identified for all compositions by X-ray diffraction analysis. The basic phase transition temperatures in these ceramics were studied over a wide temperature range. A change in symmetry from a tetragonal to cubic phase was detected, which was further proven by phonon anomalies in composition/temperature-dependent Raman spectra. The incorporation of Sr2+ into BaTiO3 (BT) lead to a shift in the phase transitions to lower temperatures, suppressing the ferroelectric properties and inducing relaxor-like behavior. Therefore, it was reasonable to suppose that the materials progressively lack long-range ordering. The initial second-harmonic generation (SHG) measurements demonstrated that the cubic phase of BST ceramics is not purely centrosymmetric over a wide temperature interval. We discussed the possible origin of the observed effects, and showed that electric field poling seems to reconstruct the structural ordering destroyed by the introduction of Sr2+ to BT. In the first approximation, substitution of Sr for larger Ba simply reduced the space for the off-central shift in Ti in the lattice and hence the domain polarization. A-site cation ordering in BST and its influence on the density of electronic states were also explored. The effect of doping with strontium ions in the BST compound on the density of electronic states was investigated using ab initio methods. As the calculations showed, doping BT with Sr2+ atoms led to an increase in the bandgap. The proposed calculations will also be used in the subsequent search for materials optimal for applications in photovoltaics.
DINA Model with Entropy Penalization
Authors: Wang Juntao , Li Yuan
Published: September 2023

The cognitive diagnosis model (CDM) is an effective statistical tool for extracting the discrete attributes of individuals based on their responses to diagnostic tests. When dealing with cases that involve small sample sizes or highly correlated attributes, not all attribute profiles may be present. The standard method, which accounts for all attribute profiles, not only increases the complexity of the model but also complicates the calculation. Thus, it is important to identify the empty attribute profiles. This paper proposes an entropy-penalized likelihood method to eliminate the empty attribute profiles. In addition, the relation between attribute profiles and the parameter space of item parameters is discussed, and two modified expectation–maximization (EM) algorithms are designed to estimate the model parameters. Simulations are conducted to demonstrate the performance of the proposed method, and a real data application based on the fraction–subtraction data is presented to showcase the practical implications of the proposed method.
An Analytic Network Process to Support Financial Decision-Making in the Context of Behavioural Finance
Authors: Martino Roberta , Ventre Viviana
Published: September 2023

Following the financial crisis of the last decade and the increasing complexity of financial products, the European Union has introduced investor protection tools that require professionals to carry out a client profiling process. The aim is to offer products that are in line with the characteristics of the individual. The classes of variables for comprehensive profiling are obtained by matching the elements proposed by the Markets in Financial Instruments Directive and studies of classical finance. However, behavioural finance studies, which emphasise the importance of behavioural attitudes, are not clearly considered in this structured profiling. The present paper discusses the implementation of an analytic network process to support financial decision-making in a behavioural context, combining regulatory guidance and qualitative and quantitative evidence from the literature. The Kersey Temperament Model is used as the behavioural model to construct the network cluster that incorporates personality into the valuation. Uncertainty management is incorporated through recent studies in the context of intertemporal choice theory. The functionality of the network is verified through a case study, where two alternatives with different characteristics are considered to meet the same investment objective. The present approach proves how the generated structure can provide strong support for financial decision-making.
Trace Formulae for Second-Order Differential Pencils with a Frozen Argument
Authors: Hu Yi-Teng , Şat Murat
Published: September 2023

This paper deals with second-order differential pencils with a fixed frozen argument on a finite interval. We obtain the trace formulae under four boundary conditions: Dirichlet–Dirichlet, Neumann–Neumann, Dirichlet–Neumann, Neumann–Dirichlet. Although the boundary conditions and the corresponding asymptotic behaviour of the eigenvalues are different, the trace formulae have the same form which reveals the impact of the frozen argument.
Accelerated Maximum Entropy Method for Time Series Models Estimation
Authors: Dubnov Yuri A. , Boulytchev Alexandr V.
Published: September 2023

The work is devoted to the development of a maximum entropy estimation method with soft randomization for restoring the parameters of probabilistic mathematical models from the available observations. Soft randomization refers to the technique of adding regularization to the functional of information entropy in order to simplify the optimization problem and speed up the learning process compared to the classical maximum entropy method. Entropic estimation makes it possible to restore probability distribution functions for model parameters without introducing additional assumptions about the likelihood function; thus, this estimation method can be used in problems with an unspecified type of measurement noise, such as analysis and forecasting of time series.
Ranking of Service Quality Index and Solutions for Online English Teaching in the Post-COVID-19 Crisis
Authors: Ma Yu-Yu , Lin Chia-Liang , Lin Hung-Lung
Published: September 2023

Online English teaching remains prevalent post-pandemic, yet there is a significant research gap in assessing service quality during this period. Thus, this study employs a hybrid FANP and GRA method to evaluate critical factors sustaining high service quality in online English teaching in the post-coronavirus era. The FANP model highlights key contributors like professional employees, trustworthy staff, flexible transaction times, and a secure transaction environment. In contrast, GRA identifies personnel quality, responsiveness to customer needs, and a secure transaction mechanism as top factors. Individual customer needs and service facilities are of less importance in both models. This study’s primary contribution is proposing an integrated FANP and GRA approach to rank potential solutions for online English teaching service quality in the post-COVID-19 fuzzy context. The findings guide the online English teaching industry in maintaining service quality in future similar scenarios.
In Situ Observations of the Strain Competition Phenomenon in Aluminum Alloy Resistance Spot Welding Joints during Lap Shear Testing
Authors: Zhang Yu , Tang Jiaxi , Liu Tong , Ma Xiaoyu , Wang Yipeng
Published: September 2023

The real-time evolution of the deformation and strain field of non-heat-treatable aluminum alloy 5754 and heat-treatable aluminum alloy 6061 resistance spot welding joints during the lap shear test was extracted using the digital image correlation (DIC) technique. The strain competition phenomenon between the nugget and its peripheral metal was quantitatively analyzed by applying 2D and 3D DIC analyses. The quantitative data show the tensile strain concentrated in the peripheral metal of the AA5754-O joint, which fractured in the pull-out mode. In comparison, a significant shear strain appears in the nugget of the AA6061-T6 joint, leading to its fracture in the interfacial failure mode during the lap shear test. The phase evolution of the nugget was analyzed using the thermodynamics database JMatPro, which was further used to calculate the local strength of the joints. The results indicate that the nugget strength of AA5754 is 223 MPa, the nugget strength of AA6061 is 178 MPa, and the heat-affected zone (HAZ) strength of AA6061 is 263 MPa. By inputting the local strength data, the calculated result of the analytical load-bearing competition model is in accordance with the experimental data of the lap shear test.
Fundamental Study on Electric Arc Furnace Steelmaking with Submerged Carbon Powder Injection with CO2-O2 Mixed Gas
Authors: Li Jianjun , Wei Guangsheng , Han Chengjin
Published: September 2023

The technology of submerged carbon powder injection with CO2 and O2 mixed gas (SCPI-COMG) is a new type of powder injection technology. It can increase the molten bath carbon content and improve the molten steel quality by injecting carbon powder directly into the molten steel with CO2 and O2 mixed gas. To optimize the process parameters of this novel technology, the mechanism of this technology and the effect of SCPI-COMG on EAF steelmaking were investigated in this study. Based on an induction furnace experiment, the effects of molten bath carburization and fluid flow on the scrap melting were analyzed. A mathematical model of the axis of gas jets in liquid steel was built to analyze the impact behaviors of gas jets in liquid steel. Based on the results of this theoretical model, for a gas jet in liquid steel, with α ≥ 20°, the horizontal inject distance decreases with α increasing and with 0° ≤ α ≤ 20°, the horizontal inject distance increases with α increasing. Finally, based on the newly built materials and energy balance model of EAF steelmaking with SCPI-COMG, the influences of the gas-solid parameters on the EAF steelmaking technical indexes were also analyzed.is very useful for optimizing the process parameters of EAF steelmaking with SCPI-COMG. The results of this study are very useful to optimize the process parameters of EAF steelmaking with SCPI-COMG of Gas Jet in Liquid Steel.
Unlocking the Potential: Amino Acids’ Role in Predicting and Exploring Therapeutic Avenues for Type 2 Diabetes Mellitus
Authors: Ding Yilan , Wang Shuangyuan , Lu Jieli
Published: September 2023

Diabetes mellitus, particularly type 2 diabetes mellitus (T2DM), imposes a significant global burden with adverse clinical outcomes and escalating healthcare expenditures. Early identification of biomarkers can facilitate better screening, earlier diagnosis, and the prevention of diabetes. However, current clinical predictors often fail to detect abnormalities during the prediabetic state. Emerging studies have identified specific amino acids as potential biomarkers for predicting the onset and progression of diabetes. Understanding the underlying pathophysiological mechanisms can offer valuable insights into disease prevention and therapeutic interventions. This review provides a comprehensive summary of evidence supporting the use of amino acids and metabolites as clinical biomarkers for insulin resistance and diabetes. We discuss promising combinations of amino acids, including branched-chain amino acids, aromatic amino acids, glycine, asparagine and aspartate, in the prediction of T2DM. Furthermore, we delve into the mechanisms involving various signaling pathways and the metabolism underlying the role of amino acids in disease development. Finally, we highlight the potential of targeting predictive amino acids for preventive and therapeutic interventions, aiming to inspire further clinical investigations and mitigate the progression of T2DM, particularly in the prediabetic stage.
Short-Term Standard Diet Consumption Prior to the Oral Fat Tolerance Test Modulates the Postprandial Triglyceride Response
Authors: Balaban Yucesan Fulya , Demir Eda Nur , Ozer Yaman Serap , Orem Asım , Dokuz Murat Busra , Bilgin Busra
Published: September 2023

We hypothesized that the consumption of a 3-day standard diet (SD) prior to the oral fat tolerance test (OFTT), used to evaluate postprandial lipemia, may counteract the undesirable effects of individual dietary habits on the test results. The OFTT was applied to 22 healthy adults (11 females and 11 males), after their habitual diets (HDs) and following the consumption of a 3-day SD (45–60% energy from carbohydrate, 20–35% from fat, and 10–20% from protein). Plasma triglyceride (TG) concentrations were measured during fasting and at the fourth hour of the OFTT. A 3-day SD significantly reduced fasting and fourth-hour TG concentrations and delta TG values by 10%, 12.8%, and 22.7%, respectively. Decreases were observed in fasting and fourth-hour TG and delta TG values following the 3-day SD compared to the HD in subjects with fasting TG concentrations between 89 and 180 mg/dL (p = 0.062, p = 0.018, and 0.047, respectively). As a result, the consumption of a 3-day standardized diet prior to the OFTT may be useful to eliminate the false positive or negative effects of individual dietary habits on test results and to correctly identify individuals who should be administered the OFTT.
Effects of Pregnancy on Plasma Sphingolipids Using a Metabolomic and Quantitative Analysis Approach
Authors: Enthoven Luke F. , Shi Yuanyuan , Fay Emily , Kim Agnes , Moreni Sue , Mao Jennie , Isoherranen Nina , Totah Rheem A. , Hebert Mary F.
Published: September 2023

Changes in the maternal metabolome, and specifically the maternal lipidome, that occur during pregnancy are relatively unknown. The objective of this investigation was to evaluate the effects of pregnancy on sphingolipid levels using metabolomics analysis followed by confirmational, targeted quantitative analysis. We focused on three subclasses of sphingolipids: ceramides, sphingomyelins, and sphingosines. Forty-seven pregnant women aged 18 to 50 years old participated in this study. Blood samples were collected on two study days for metabolomics analysis. The pregnancy samples were collected between 25 and 28 weeks of gestation and the postpartum study day samples were collected ≥3 months postpartum. Each participant served as their own control. These samples were analyzed using a Ultra-performance liquid chromatography/mass spectroscopy/mass spectroscopy (UPLC/MS/MS) assay that yielded semi-quantitative peak area values that were used to compare sphingolipid levels between pregnancy and postpartum. Following this lipidomic analysis, quantitative LC/MS/MS targeted/confirmatory analysis was performed on the same study samples. In the metabolomic analysis, 43 sphingolipid metabolites were identified and their levels were assessed using relative peak area values. These profiled sphingolipids fell into three categories: ceramides, sphingomyelins, and sphingosines. Of the 43 analytes measured, 35 were significantly different during pregnancy (p < 0.05) (including seven ceramides, 26 sphingomyelins, and two sphingosines) and 32 were significantly higher during pregnancy compared to postpartum. Following metabolomics, a separate quantitative analysis was performed and yielded quantified concentration values for 23 different sphingolipids, four of which were also detected in the metabolomics study. Quantitative analysis supported the metabolomics results with 17 of the 23 analytes measured found to be significantly different during pregnancy including 11 ceramides, four sphingomyelins, and two sphingosines. Fourteen of these were significantly higher during pregnancy. Our data suggest an overall increase in plasma sphingolipid concentrations with possible implications in endothelial function, gestational diabetes mellitus (GDM), intrahepatic cholestasis of pregnancy, and fetal development. This study provides evidence for alterations in maternal sphingolipid metabolism during pregnancy.
Applications of a Novel Tunable Piezoelectric Vibration Energy Harvester
Authors: Raghavan Sreekumari , Gupta Rishi , Sharma Loveleen
Published: September 2023

Conversion of ambient energy to usable electrical energy is attracting attention from researchers since providing a maintenance-free power source for the sensors is critical in any IoT (Internet of Things)-based system and in SHM (structural health monitoring). Continuous health monitoring of structures is advantageous since the damage can be identified at inception and the necessary action taken. Sensor technology has advanced significantly, and MEMS (microelectromechanical systems)-based low-power sensors are available for incorporating into large structures. Relevant signal conditioning and transmission modules have also evolved, making them power-efficient and miniaturized. Various micro wireless sensor nodes (WSN) have also been developed in recent years that require very little power. This paper describes the applications of a novel tunable piezoelectric vibration energy harvester (PVEH) for providing autonomous power to low-power MEMS sensors for use in IoT and remote SHM. The novel device uses piezoelectric material and an ionic polymer–metal composite (IPMC) and enables electrical tuning of the resonant frequency using a small portion of the power generated.
Raman Lasing in a Tellurite Microsphere with Thermo-Optical on/off Switching by an Auxiliary Laser Diode
Authors: Anashkina Elena A. , Marisova Maria P. , Dorofeev Vitaly V. , Andrianov Alexey V.
Published: September 2023

The generation of coherent light based on inelastic stimulated Raman scattering in photonic microresonators has been attracting great interest in recent years. Tellurite glasses are promising materials for such microdevices since they have large Raman gain and large Raman frequency shift. We experimentally obtained Raman lasing at a wavelength of 1.8 µm with a frequency shift of 27.5 THz from a 1.54 µm narrow-line pump in a 60 µm tellurite glass microsphere with a Q-factor of 2.5 × 107. We demonstrated experimentally a robust, simple, and cheap way of thermo-optically controlled on/off switching of Raman lasing in a tellurite glass microsphere by an auxiliary laser diode. With a permanently operating narrow-line pump laser, on/off switching of the auxiliary 405 nm laser diode led to off/on switching of Raman generation. We also performed theoretical studies supporting the experimental results. The temperature distribution and thermal frequency shifts in eigenmodes in the microspheres heated by the thermalized power of an auxiliary diode and the partially thermalized power of a pump laser were numerically simulated. We analyzed the optical characteristics of Raman generation in microspheres of different diameters. The numerical results were in good agreement with the experimental ones.
Metagenomic Analysis of a Continuous-Flow Aerobic Granulation System for Wastewater Treatment
Authors: Gomeiz Alison T. , Sun Yewei , Newborn Aaron , Wang Zhi-Wu , Angelotti Bob , Van Aken Benoit
Published: September 2023

Aerobic granulation is an emerging process in wastewater treatment that has the potential to accelerate sedimentation of the microbial biomass during secondary treatment. Aerobic granulation has been difficult to achieve in the continuous flow reactors (CFRs) used in modern wastewater treatment plants. Recent research has demonstrated that the alternation of nutrient-abundant (feast) and nutrient-limiting (famine) conditions is able to promote aerobic granulation in a CFR. In this study, we conducted a metagenomic analysis with the objective of characterizing the bacterial composition of the granular biomass developed in three simulated plug flow reactors (PFRs) with different feast-to-famine ratios. Phylogenetic analyses revealed a clear distinction between the bacterial composition of aerobic granules in the pilot simulated PFRs as compared with conventional activated sludge. Larger and denser granules, showing improved sedimentation properties, were observed in the PFR with the longest famine time and were characterized by a greater proportion of bacteria producing abundant extracellular polymeric substances (EPS). Functional metagenomic analysis based on KEGG pathways indicated that the large and dense aerobic granules in the PFR with the longest famine time showed increased functionalities related to secretion systems and quorum sensing, which are characteristics of bacteria in biofilms and aerobic granules. This study contributes to a further understanding of the relationship between aerobic granule morphology and the bacterial composition of the granular biomass.
The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks in Pseudomonas aeruginosa
Authors: Juříková Tereza , Mácha Hynek , Lupjanová Vanda , Pluháček Tomáš , Marešová Helena , Papoušková Barbora , Luptáková Dominika , Patil Rutuja H. , Benada Oldřich , Grulich Michal , Palyzová Andrea
Published: September 2023

Pseudomonas aeruginosa is recognized as a significant cause of morbidity and mortality among nosocomial pathogens. In respiratory infections, P. aeruginosa acts not only as a single player but also collaborates with the opportunistic fungal pathogen Aspergillus fumigatus. This study introduced a QS molecule portfolio as a potential new biomarker that affects the secretion of virulence factors and biofilm formation. The quantitative levels of QS molecules, including 3-o-C12-HSL, 3-o-C8-HSL, C4-HSL, C6-HSL, HHQ, PQS, and PYO, measured using mass spectrometry in a monoculture, indicated metabolic changes during the transition from planktonic to sessile cells. In the co-cultures with A. fumigatus, the profile of abundant QS molecules was reduced to 3-o-C12-HSL, C4-HSL, PQS, and PYO. A decrease in C4-HSL by 50% to 170.6 ± 11.8 ng/mL and an increase 3-o-C12-HSL by 30% up to 784.4 ± 0.6 ng/mL were detected at the stage of the coverage of the hyphae with bacteria. Using scanning electron microscopy, we showed the morphological stages of the P. aeruginosa biofilm, such as cell aggregates, maturated biofilm, and cell dispersion. qPCR quantification of the genome equivalents of both microorganisms suggested that they exhibited an interplay strategy rather than antagonism. This is the first study demonstrating the quantitative growth-dependent appearance of QS molecule secretion in a monoculture of P. aeruginosa and a co-culture with A. fumigatus.
Exploring Peracetic Acid and Acidic pH Tolerance of Antibiotic-Resistant Non-Typhoidal Salmonella and Enterococcus faecium from Diverse Epidemiological and Genetic Backgrounds
Authors: Rebelo Andreia , Duarte Bárbara , Freitas Ana R. , Peixe Luísa , Antunes Patrícia , Novais Carla
Published: September 2023

Acid stress poses a common challenge for bacteria in diverse environments by the presence of inorganic (e.g., mammals’ stomach) or organic acids (e.g., feed additives; acid-based disinfectants). Limited knowledge exists regarding acid-tolerant strains of specific serotypes, clonal lineages, or sources in human/animal pathogens: namely, non-typhoidal Salmonella enterica (NTS) and Enterococcus faecium (Efm). This study evaluated the acidic pH (Mueller–Hinton acidified with HCl) and peracetic acid (PAA) susceptibility of Efm (n = 72) and NTS (n = 60) from diverse epidemiological/genetic backgrounds and with multiple antibiotic resistance profiles. Efm minimum growth/survival pH was 4.5–5.0/3.0–4.0, and for NTS it was 4.0–4.5/3.5–4.0. Efm distribution among acidic pH values showed that only isolates of clade-non-A1 (non-hospital associated) or the food chain were more tolerant to acidic pH compared to clade-A1 (hospital-associated clones) or clinical isolates (p < 0.05). In the case of NTS, multidrug-resistant (MDR) isolates survived better in acidic pH (p < 0.05). The PAA MIC/MBC for Efm was 70–120/80–150 mg/L, and for NTS, it was 50–70/60–100 mg/L. The distribution of Efm among PAA concentrations showed that clade-A1 or MDR strains exhibited higher tolerance than clade-non-A1 or non-MDR ones (p < 0.05). NTS distribution also showed higher tolerance to PAA among non-MDR and clinical isolates than food chain ones (p < 0.05) but there were no differences among different serogroups. This unique study identifies specific NTS or Efm populations more tolerant to acidic pH or PAA, emphasizing the need for further research to tailor controlled measures of public health and food safety within a One Health framework.
Emerging and Novel Viruses in Passerine Birds
Authors: Williams Richard A. J. , Sánchez-Llatas Christian J. , Doménech Ana , Madrid Ricardo , Fandiño Sergio , Cea-Callejo Pablo , Gomez-Lucia Esperanza , Benítez Laura
Published: September 2023

There is growing interest in emerging viruses that can cause serious or lethal disease in humans and animals. The proliferation of cloacal virome studies, mainly focused on poultry and other domestic birds, reveals a wide variety of viruses, although their pathogenic significance is currently uncertain. Analysis of viruses detected in wild birds is complex and often biased towards waterfowl because of the obvious interest in avian influenza or other zoonotic viruses. Less is known about the viruses present in the order Passeriformes, which comprises approximately 60% of extant bird species. This review aims to compile the most significant contributions on the DNA/RNA viruses affecting passerines, from traditional and metagenomic studies. It highlights that most passerine species have never been sampled. Especially the RNA viruses from Flaviviridae, Orthomyxoviridae and Togaviridae are considered emerging because of increased incidence or avian mortality/morbidity, spread to new geographical areas or hosts and their zoonotic risk. Arguably poxvirus, and perhaps other virus groups, could also be considered “emerging viruses”. However, many of these viruses have only recently been described in passerines using metagenomics and their role in the ecosystem is unknown. Finally, it is noteworthy that only one third of the viruses affecting passerines have been officially recognized.
The Mineralogy, Geochemistry and Origin of the Supergene Manganese Occurrences in the Southern Minas Gerais, Brazil
Authors: Parrotti Davi Diorio , da Conceição Fabiano Tomazini , Navarro Guillermo Rafael Beltran
Published: September 2023

In equatorial and tropical regions, supergene mineral deposits created during water/rock interactions are found. Simply put, these supergene deposits are formed through the accumulation of low solubility ions or through the preservation of primary minerals. The supergene manganese (Mn) deposits are examples of the economic importance associated with the chemical weathering processes. In Brazil, the Southern Brasilia Orogen (SBO) was generated during the collision between the Paranapanema Craton and the passive margin of the São Francisco Craton. In the southern Minas Gerais (MG), several supergene Mn occurrences are hosted in the SBO, which were originated during the chemical weathering of gondites belonging to the Amparo Complex. Here, we studied the supergene Mn occurrences in the southern MG, more specifically in the municipalities of Ouro Fino and Careaçu. The MnO contents ranged from 25.50 to 28.40 wt% at Ouro Fino and from 16.80 to 21.20 wt% at Careaçu. These supergene Mn deposits have a diverse mineral assemblage, being composed of spessartine, quartz, Mn-oxides, goethite and kaolinite. The various Mn minerals formed due to spessartine incongruent dissolution were hollandites, cryptomelanes, romanechites, pyrolusites and lithiophorites. Both study areas are relevant for the possible opening of mines for the commercialization of Mn.
Direct Tensile Measurement for Cemented Paste Backfill
Authors: Pan Andrew , Grabinsky Murray
Published: September 2023

Tensile strength is a crucial parameter involved in the design and analysis of cemented paste backfill (CPB). The ability of CPB to withstand tensile forces is essential for the stability of the backfilled stopes, particularly in areas with high stress or deformation. The tensile strength is a critical design parameter used in sill mats to perform underhand cut-and-fill operations. This study presents a novel technique that utilizes rectangular dog-bone specimens and compression to tensile load converters to perform the direct determination of tensile strength. This study indicates that the prevailing assumption regarding the ratio of unconfined compressive strength (UCS) to tensile strength (i.e., 10:1 or 12:1) underestimates the strength. The results suggest a ratio closer to 3:1 or 4:1. The findings indicate that the ratio varies with the curing interval. Specifically, the tensile-to-compressive strength ratios were higher in early-age specimens, as tensile strength values do not increase at the same rate as those of compressive strength. This disparity has notable implications, as underestimating tensile strength via traditional UCS-to-tensile strength ratios could potentially inflate binder consumption. Our study underscores the importance of using direct tensile strength measurements to optimize mining operations.