bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Silvicultural Treatments on Nothofagus glauca Forests Regarding Canopy Structure Patterns, Solar Radiation Transmission, and Tree Regeneration
Authors: Santelices-Moya Rómulo , Promis Álvaro , Peralta-Aguilera Miguel , Cabrera-Ariza Antonio M.
Published: January 2024

This study focuses on the natural regeneration of Nothofagus glauca, a critical component of temperate forests in the Mediterranean region of the Southern Hemisphere in Chile. Various harvesting and canopy opening methods were evaluated to understand their impact on the regeneration of this species. A “mast seeding” behavior was observed in seed production, with alternating years of high and low production. Seed viability was affected by lepidopteran infestation and extreme weather events. The results indicate variable seed quantity and quality, influencing regeneration. First-year seedling mortality was significant, reaching up to 95%, and regeneration decreased annually. Additionally, changes in canopy opening and solar radiation transmission were analyzed, revealing significant post-harvest modifications. This study provides valuable insights for the sustainable management of Mediterranean forests, emphasizing the importance of considering seed production variability and regeneration dynamics in decision-making processes.
Evolutionary Characteristics and Driving Forces of Green Space in Guangzhou from a Zoning Perspective
Authors: Wang Huimin , Lin Canrui , Ou Sihua , Feng Qianying , Guo Kui , Xie Jiazhou , Wei Xiaojian
Published: January 2024

Urban green spaces provide very important environmental and social services. Their dynamic changes are driven by a combination of natural and socioeconomic factors. However, the coupling mechanism of these factors has not been systematically studied. In this study, we examined dynamic characteristics of green space in Guangzhou from different zoning perspectives and explored the regional heterogeneity of the individual and interactive effects of driving factors using the geographic detector. The results showed that (1) from 2000 to 2020, the annual change rate of green space area in the central area was more apparent than that in the suburban areas. The fragmentation of green space in the near suburbs had increased. (2) Changes in green space were influenced by the combination of topography, climate, and socioeconomic development. From 2010 to 2020, the expansion of built-up land and GDP growth gradually had a substantial effect on the change in green space in the central area and the near suburbs. (3) The q-values of the interaction detections of the geodetector showed that the explanatory power of most factor interactions exceeded that of individual factors. Green space in the central area was primarily influenced by the interaction of economic and built-up land expansion. In contrast, green space in the near suburbs was mainly influenced by the interaction of urban expansion and topography and climate. Green space in the far suburbs was mainly affected by climate factors and human activity intensity. The results and methods of this study can provide decision support for the zoning planning of urban green space system in other cities or regions.
Future Sustainable Internet Energy-Defined Networking
Authors: Galis Alex
Published: January 2024

This paper presents a comprehensive set of design methods for making future Internet networking fully energy-aware and sustainably minimizing and managing the energy footprint. It includes (a) 41 energy-aware design methods, grouped into Service Operations Support, Management Operations Support, Compute Operations Support, Connectivity/Forwarding Operations Support, Traffic Engineering Methods, Architectural Support for Energy Instrumentation, and Network Configuration; (b) energy consumption models and energy metrics are identified and specified. It specifies the requirements for energy-defined network compliance, which include energy-measurable network devices with the support of several control messages: registration, discovery, provisioning, discharge, monitoring, synchronization, flooding, performance, and pushback.
Unveiling the Roles of LncRNA MOIRAs in Rice Blast Disease Resistance
Authors: Liu Qing , Xue Jiao , Zhang Lanlan , Jiang Liqun , Li Chen
Published: January 2024

Rice blast disease, caused by the fungal pathogen Magnaporthe oryzae, is a major threat to rice production worldwide. This study investigates the role of long non-coding RNAs (lncRNAs) in rice’s response to this destructive disease, with a focus on their impacts on disease resistance and yield traits. Three specific lncRNAs coded by M. oryzae infection-responsive lncRNAs (MOIRAs), MOIRA1, MOIRA2, and MOIRA3, were identified as key regulators of rice’s response to M. oryzae infection. Strikingly, when MOIRA1 and MOIRA2 were overexpressed, they exhibited a dual function: they increased rice’s susceptibility to blast fungus, indicating a negative role in disease resistance, while simultaneously enhancing tiller numbers and single-plant yield, with no adverse effects on other yield-related traits. This unexpected improvement in productivity suggests the possibility of overcoming the traditional trade-off between disease resistance and crop yield. These findings provide a novel perspective on crop enhancement, offering a promising solution to global food security challenges by developing rice varieties that effectively balance disease resistance and increased productivity.
Glacial Lakes of Mongolia
Authors: Walther Michael , Kamp Ulrich , Nandintsetseg Nyam-Osor , Dashtseren Avirmed , Temujin Khurelbaatar
Published: January 2024

The over 2200 lakes of Mongolia are generally poorly studied, particularly the glacial lakes. This overview study presents a classification of the glacial lakes based on tectonic-geological and geomorphological dynamics. Selected representative lakes are described using results from fieldwork and satellite image analysis, including bathymetry, paleoshorelines, and recent lake-level fluctuations between 1987 and 2020. Generally, lake levels dropped from the early Holocene until recently, with the onset of the climate change-driven glacier recession that has resulted in lake-level rises and area expansion in almost all moraine-dammed, tongue-basin, and ice-contact lakes. In contrast, endorheic lakes have mainly been shrinking for the past forty years because of an increase in air temperature and evaporation rates and the effects of an intensifying water use within the catchment for irrigation, mining, and hydroelectric energy production in the form of dams. The creation of a lake monitoring system based on an in-depth inventory is recommended.
Locating Senior-Friendly Restaurants in a Community: A Bi-Objective Optimization Approach for Enhanced Equality and Convenience
Authors: Yang Shuyan , Li Changfeng , Mu Wangshu
Published: January 2024

Senior-friendly restaurants are dining establishments that cater specifically to the needs and preferences of older adults in a community. As the physical capabilities of seniors progressively decline and their activity spaces contract over time, determining optimal locations for such restaurants to ensure their accessibility becomes crucial. Nevertheless, the criteria for the location selection of senior-friendly restaurants are multifaceted, necessitating the consideration of both equality and convenience. First, these restaurants often receive government funding, which means that equitable access should be guaranteed for all community residents. Second, the daily activity patterns of seniors should be accounted for. Therefore, these restaurants should be situated in close proximity to other essential facilities utilized by seniors, such as recreational facilities that accommodate routine postmeal activities. Despite the long-standing application of spatial optimization approaches to facility location issues, no existing models directly address the location selection of senior-friendly restaurants. This study introduces a bi-objective optimization model, the Community Senior-Friendly Restaurants Location Problem (CSRLP), designed to determine optimal locations for senior-friendly restaurants, taking into consideration both service coverage and proximity to recreational facilities simultaneously. We formulated the CSRLP as an integer linear programming model. Simulation tests indicate that the CSRLP can be solved both effectively and efficiently. Applying the CSRLP model to two communities in Dongcheng District, Beijing, China, we explored Pareto optimal solutions, facilitating the selection of senior-friendly restaurant locations under diverse scenarios. The results highlight the significant value of spatial optimization in aiding senior-friendly restaurant location planning and underscore key policy implications.
Genetic Model Identification and Major QTL Mapping for Petiole Thickness in Non-Heading Chinese Cabbage
Authors: Liu Guangyuan , Li Yongkuan , Si Jia , Lu Rong , Hui Maixia
Published: January 2024

Petioles of non-heading Chinese cabbage are not only an important edible part but also a conduit for nutrient transport, holding significant agricultural and research value. In this study, we conducted a comprehensive genetic analysis of petiole-related traits using a segregating population. Modern quantitative genetic approaches were applied to investigate the genetic regulation of petiole thickness. The results indicated that petiole thickness is a quantitative trait, and the identified genetic model was consistent with two pairs of additive-dominant main genes and additive-dominant polygenes (2MG-AD). BSA-seq analysis identified a major effect of QTL controlling petiole thickness on chromosome A09: 42.08–45.09 Mb, spanning 3.01 Mb, designated as QTL-BrLH9. Utilizing InDel markers, the interval was narrowed down to 51 kb, encompassing 14 genes with annotations for 10 of them. Within the interval, four mutated genes were detected. Combined with gene annotation, protein sequence analysis, and homology alignment, it was found that BraA09g063520.3C’s homologous gene SMXL6 in Arabidopsis (Arabidopsis thaliana (L.) Heynh) is an inhibitor of the coding and synthesis of the strigolactone pathway. Strigolactone (SLs) plays an important role in plant growth and development. The cloning results showed that multiple frameshift mutations and non-synonymous mutations occurred on the exon. The qPCR results showed that the expression of the gene was significantly different between the two parents at the adult stage, so it was speculated that it would lead to changes in petiole thickness. BraA09g063520.3C was predicted as the final candidate gene.
Enhancing Human Cutaneous Wound Healing through Targeted Suppression of Large Conductance Ca2+-Activated K+ Channels
Authors: Choi Chang-Rok , Kim Eun-Jin , Choi Tae Hyun , Han Jaehee , Kang Dawon
Published: January 2024

The modulation of K+ channels plays a crucial role in cell migration and proliferation, but the effect of K+ channels on human cutaneous wound healing (CWH) remains underexplored. This study aimed to determine the necessity of modulating K+ channel activity and expression for human CWH. The use of 25 mM KCl as a K+ channel blocker markedly improved wound healing in vitro (in keratinocytes and fibroblasts) and in vivo (in rat and porcine models). K+ channel blockers, such as quinine and tetraethylammonium, aided in vitro wound healing, while Ba2+ was the exception and did not show similar effects. Single-channel recordings revealed that the Ba2+-insensitive large conductance Ca2+-activated K+ (BKCa) channel was predominantly present in human keratinocytes. NS1619, an opener of the BKCa channel, hindered wound healing processes like proliferation, migration, and filopodia formation. Conversely, charybdotoxin and iberiotoxin, which are BKCa channel blockers, dramatically enhanced these processes. The downregulation of BKCa also improved CWH, whereas its overexpression impeded these healing processes. These findings underscore the facilitative effect of BKCa channel suppression on CWH, proposing BKCa channels as potential molecular targets for enhancing human cutaneous wound healing.
Lupus Nephritis Biomarkers: A Critical Review
Authors: Alduraibi Fatima K. , Tsokos George C.
Published: January 2024

Lupus nephritis (LN), a major complication in individuals diagnosed with systemic lupus erythematosus, substantially increases morbidity and mortality. Despite marked improvements in the survival of patients with severe LN over the past 50 years, complete clinical remission after immunosuppressive therapy is achieved in only half of the patients. Therefore, timely detection of LN is vital for initiating prompt therapeutic interventions and improving patient outcomes. Biomarkers have emerged as valuable tools for LN detection and monitoring; however, the complex role of these biomarkers in LN pathogenesis remains unclear. Renal biopsy remains the gold standard for the identification of the histological phenotypes of LN and guides disease management. However, the molecular pathophysiology of specific renal lesions remains poorly understood. In this review, we provide a critical, up-to-date overview of the latest developments in the field of LN biomarkers.
A Comprehensive Analysis of Auxin Response Factor Gene Family in Melastoma dodecandrum Genome
Authors: Peng Yukun , Zhao Kai , Zheng Ruiyue , Chen Jiemin , Zhu Xuanyi , Xie Kai , Huang Ruiliu , Zhan Suying , Su Qiuli , Shen Mingli , Niu Muqi , Chen Xiuming , Peng Donghui , Ahmad Sagheer , Liu Zhong-Jian , Zhou Yuzhen
Published: January 2024

Auxin Response Factors (ARFs) mediate auxin signaling and govern diverse biological processes. However, a comprehensive analysis of the ARF gene family and identification of their key regulatory functions have not been conducted in Melastoma dodecandrum, leading to a weak understanding of further use and development for this functional shrub. In this study, we successfully identified a total of 27 members of the ARF gene family in M. dodecandrum and classified them into Class I–III. Class II–III showed more significant gene duplication than Class I, especially for MedARF16s. According to the prediction of cis-regulatory elements, the AP2/ERF, BHLH, and bZIP transcription factor families may serve as regulatory factors controlling the transcriptional pre-initiation expression of MedARF. Analysis of miRNA editing sites reveals that miR160 may play a regulatory role in the post-transcriptional expression of MeARF. Expression profiles revealed that more than half of the MedARFs exhibited high expression levels in the stem compared to other organs. While there are some specific genes expressed only in flowers, it is noteworthy that MedARF16s, MedARF7A, and MedARF9B, which are highly expressed in stems, also demonstrate high expressions in other organs of M. dodecandrum. Further hormone treatment experiments revealed that these MedARFs were sensitive to auxin changes, with MedARF6C and MedARF7A showing significant and rapid changes in expression upon increasing exogenous auxin. In brief, our findings suggest a crucial role in regulating plant growth and development in M. dodecandrum by responding to changes in auxin. These results can provide a theoretical basis for future molecular breeding in Myrtaceae.
Could Targeting NPM1c+ Misfolding Be a Promising Strategy for Combating Acute Myeloid Leukemia?
Authors: Florio Daniele , Marasco Daniela
Published: January 2024

Acute myeloid leukemia (AML) is a heterogeneous group of diseases classified into various types on the basis of distinct features concerning the morphology, cytochemistry and cytogenesis of leukemic cells. Among the different subtypes, the group “AML with gene mutations” includes the variations of the gene of the multifunctional protein nucleophosmin 1 (NPM1). These mutations are the most frequent (~30–35% of AML adult patients and less in pediatric ones) and occur predominantly in the C-terminal domain (CTD) of NPM1. The most important mutation is the insertion at W288, which determines the frame shift W288Cfs12/Ffs12/Lfs*12 and leads to the addition of 2–12 amino acids, which hamper the correct folding of NPM1. This mutation leads to the loss of the nuclear localization signal (NoLS) and to aberrant cytoplasmic localization, denoted as NPM1c+. Many investigations demonstrated that interfering with the cellular location and oligomerization status of NPM1 can influence its biological functions, including the proper buildup of the nucleolus, and therapeutic strategies have been proposed to target NPM1c+, particularly the use of drugs able to re-direct NPM1 localization. Our studies unveiled a direct link between AML mutations and the neat amyloidogenic character of the CTDs of NPM1c+. Herein, with the aim of exploiting these conformational features, novel therapeutic strategies are proposed that rely on the induction of the selective self-cytotoxicity of leukemic blasts by focusing on agents such as peptides, peptoids or small molecules able to enhance amyloid aggregation and targeting selectively AML–NPM1c+ mutations.
Chemical Constituents and Anticancer Activities of the Extracts from Phlomis × commixta Rech. f. (P. cretica × P. lanata)
Authors: Stefanakis Michalis K. , Tsiftsoglou Olga St. , Mašković Pavle Z. , Lazari Diamanto , Katerinopoulos Haralambos E.
Published: January 2024

The present work is the first report on the ingredients of the P. × commixta hybrid, a plant of the genus Phlomis. So far, thirty substances have been isolated by various chromatographic techniques and identified by spectroscopic methods, such as UV/Vis, NMR, GC-MS and LC-MS. The compounds are classified as flavonoids: naringenin, eriodyctiol, eriodyctiol-7-O-β-D-glucoside, luteolin, luteolin-7-O-β-D-glucoside, apigenin, apigenin-7-O-β-D-glucoside, diosmetin-7-O-β-D-glucoside, quercetin, hesperetin and quercetin-3-O-β-D-glucoside; phenylpropanoids: martynoside, verbascoside, forsythoside B, echinacoside and allysonoside; chromene: 5,7-dihydroxychromone; phenolic acids: caffeic acid, p-hydroxybenzoic acid, chlorogenic acid, chlorogenic acid methyl ester, gallic acid, p-coumaric acid and vanillic acid; aliphatic hydrocarbon: docos-1-ene; steroids: brassicasterol and stigmasterol; a glucoside of allylic alcohol, 3-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranosyl-oct-1-ene-3-ol, was fully characterized as a natural product for the first time. Two tyrosol esters were also isolated: tyrosol lignocerate and tyrosol methyl ether palmitate, the latter one being isolated as a natural product for the first time. Moreover, the biological activities of the extracts from the different polarities of the roots, leaves and flowers were estimated for their cytotoxic potency. All root extracts tested showed a high cytotoxic activity against the Hep2c and RD cell lines.
The Importance of Complement-Mediated Immune Signaling in Alzheimer’s Disease Pathogenesis
Authors: Batista André F. , Khan Khyrul A. , Papavergi Maria-Tzousi , Lemere Cynthia A.
Published: January 2024

As an essential component of our innate immune system, the complement system is responsible for our defense against pathogens. The complement cascade has complex roles in the central nervous system (CNS), most of what we know about it stems from its role in brain development. However, in recent years, numerous reports have implicated the classical complement cascade in both brain development and decline. More specifically, complement dysfunction has been implicated in neurodegenerative disorders, such as Alzheimer’s disease (AD), which is the most common form of dementia. Synapse loss is one of the main pathological hallmarks of AD and correlates with memory impairment. Throughout the course of AD progression, synapses are tagged with complement proteins and are consequently removed by microglia that express complement receptors. Notably, astrocytes are also capable of secreting signals that induce the expression of complement proteins in the CNS. Both astrocytes and microglia are implicated in neuroinflammation, another hallmark of AD pathogenesis. In this review, we provide an overview of previously known and newly established roles for the complement cascade in the CNS and we explore how complement interactions with microglia, astrocytes, and other risk factors such as TREM2 and ApoE4 modulate the processes of neurodegeneration in both amyloid and tau models of AD.
The Natural History of SARS-CoV-2-Incurred Disease: From Infection to Long COVID
Authors: Liang Kung-Hao , Teng Yuan-Chi , Liao Yi-Ting , Yarmishyn Aliaksandr A. , Chiang Su-Hua , Hung Wei-Chun , Hsiao Chun-Yen , Tsai En-Tung , Chang Tai-Jay , Yang De-Ming , Wang Mong-Lien
Published: January 2024

The coronavirus SARS-CoV-2 is the causative pathogen of the COVID-19 pandemic that has been causing global upheaval since 2019. The widespread administration of vaccines has partially deterred the spread of SARS-CoV-2, yet the virus is mutating its genome to reduce its antigenicity and evade the human herd immunity. It seems that SARS-CoV-2 will co-exist with the human population for many decades to come. While most infected individuals only experience mild to moderate symptoms, some develop severe pulmonary and systemic disease that can result in hospitalization or even death. The natural history model of SARS-CoV-2 infection has been proposed which includes three sequential stages: the early infection stage, pulmonary stage, and hyper-inflammatory stage. Recently, it has been observed that many people who recovered from an acute infection still experience persistent symptoms for weeks or months, a condition known as long COVID. Furthermore, some COVID-19 patients display escalated rates of both macro- and micro-thrombosis due to endotheliopathy. Hence, we added the thrombosis and convalescent stages to the natural history model, encompassing the entire period from early infection to long COVID. The early infection stage is characterized by symptomatic or asymptomatic elevation of viral titers. Some patients progress to the pulmonary stage characterized by opacities in chest X-rays and computed tomography. The thrombosis stage is characterized by heightened rates of pulmonary thrombosis and consistently elevated D-dimer levels. The hyper-inflammatory stage is characterized by storms of cytokines, such as IL-6, IL-17, and interferons, which is a systemic effect. In the convalescent stage, some people recover completely, while others suffer from long COVID with persistent symptoms such as fatigue, shortness of breath, or brain fog. The natural history model of SARS-CoV-2 infection can be used to elucidate treatment and care.
Streamlining Temporal Formal Verification over Columnar Databases
Authors: Bergami Giacomo
Published: January 2024

Recent findings demonstrate how database technology enhances the computation of formal verification tasks expressible in linear time logic for finite traces (LTLf). Human-readable declarative languages also help the common practitioner to express temporal constraints in a straightforward and accessible language. Notwithstanding the former, this technology is in its infancy, and therefore, few optimization algorithms are known for dealing with massive amounts of information audited from real systems. We, therefore, present four novel algorithms subsuming entire LTLf expressions while outperforming previous state-of-the-art implementations on top of KnoBAB, thus postulating the need for the corresponding, leading to the formulation of novel xtLTLf-derived algebraic operators.
Improving the Flexural Response of Timber Beams Using Externally Bonded Carbon Fiber-Reinforced Polymer (CFRP) Sheets
Authors: Mansour Walid , Li Weiwen , Wang Peng , Fame Cheikh Makhfouss , Tam Lik-ho , Lu Yao , Sobuz Md. Habibur Rahman , Elwakkad Noha Yehia
Published: January 2024

This paper presents a numerical investigation of the flexural behavior of timber beams externally strengthened with carbon-fiber-reinforced polymer (CFRP) sheets. At first, the accuracy of linear elastic and elastic-plastic models in predicting the behavior of bare timber beams was compared. Then, two modeling approaches (i.e., the perfect bond method and progressive damage technique using the cohesive zone model (CZM)) were considered to simulate the interfacial behavior between FRP and timber. The models were validated against published experimental data, and the most accurate numerical procedure was identified and subsequently used for a parametric study. The length of FRP sheets varied from 50% to 100% of the total length of the beam, while different FRP layers were considered. Moreover, the effects of two strengthening configurations (i.e., FRP attached in the tensile zone only and in both the tensile and compressive zones) on load-deflection response, flexural strength, and flexural rigidity were considered. The results showed that elastic-plastic models are more accurate than linear elastic models in predicting the flexural strength and failure patterns of bare timber beams. In addition, with increasing FRP length, the increase in flexural strength ranged from 10.3% to 52.9%, while no further increase in flexural strength could be achieved beyond an effective length of 80% of the total length of the beam. Attaching the FRP to both the tensile and compressive zone was more effective in enhancing the flexural properties of the timber beam than attaching the FRP to the tensile zone only.
Influence of Laser Energy Density on Tribological Properties of AlSi10Mg Manufactured by Selective Laser Melting
Authors: Wang Keqing
Published: January 2024

In recent years, much work related to the performance of AlSi10Mg manufactured by selective laser melting (SLM) has been extensively researched. However, the study of tribological performance caused by different laser energy densities is still insufficient. This work concentrates on the relationship between the wear resistance and laser energy density of AlSi10Mg processed using SLM. Moreover, XRD characterization, density, surface roughness and microhardness were also examined since they are closely related to wear resistance. The results revealed that the XRD pattern of AlSi10Mg was mainly composed of the α-Al and Si phases under the conditions of different laser energy densities. In addition, the peak of Mg2Si was also detected. Also, the grain size increased with the increasing of laser energy density. The increase in laser energy density led to an increase in the convection and porous phenomenon in the molten pool. However, when the value was lower, the overlapping area reduced, and the strength between adjacent melting paths was insufficient, resulting in the declination of the sample property. According to the experimental results, a laser energy density of 63.33 J/mm3 was considered to be a relative optimal condition. The relative density, Ra, microhardness and wear volume were 99.2%, 8.86 μm, 128.3 HV0.2 and 2.96 × 10−2 mm3, respectively. The worn surface morphology also confirmed the influence of laser energy density on wear resistance. A regression model was established and analyzed, which showed the reliability of the results. Furthermore, the tribological mechanism was also revealed.
Preparation and Oil Adsorption of Cellulose-graft-poly(butyl acrylate-N,N′-methylene Bisacrylamide)
Authors: Guo Peng-Xiang , Wang Xin-Gang , Yang Mao-Qian , Wang Jian-Xin , Meng Fan-Jun
Published: January 2024

With the advancement of industrial economies, incidents involving spills of petroleum products have become increasingly frequent. The resulting pollutants pose significant threats to air, water, soil, plant and animal survival, as well as human health. In this study, microcrystalline cellulose served as the matrix and benzoyl peroxide (BPO) as the initiator, while butyl acrylate (BA) and N,N′-methylene bisacrylamide (MBA) were employed as graft monomers. Through free radical graft polymerization, cellulose-graft-poly(butyl acrylate-N,N′-methylene bisacrylamide) [Cell-g-P(BA-MBA)], possessing oil-adsorbing properties, was synthesized. The chemical structure, elemental composition, surface morphology and wetting properties of the graft polymerization products have been characterized, using infrared spectroscopy, elemental analysis, scanning electron microscopy and contact angle testing. The adsorption properties of Cell-g-P(BA-MBA) for various organic solvents and oils were then assessed. The experimental results demonstrated that Cell-g-P(BA-MBA) exhibited a maximum adsorption capacity of 37.55 g/g for trichloromethane. Adsorption kinetics experiments indicated a spontaneous and exothermic process involving physical adsorption, conforming to the Freundlich isotherm model. Furthermore, adsorption kinetics experiments revealed that Cell-g-P(BA-MBA) displayed favorable reuse and regeneration performance, maintaining its adsorption capacity essentially unchanged over fifteen adsorption–desorption cycles.
Epitaxy of (11–22) AlN Films on a Sputtered Buffer Layer with Different Annealing Temperatures via Hydride Vapour Phase Epitaxy
Authors: Yan Xuejun , Sun Maosong , Ji Jianli , He Zhuokun , Zhang Jicai , Sun Wenhong
Published: January 2024

AlN epilayers were grown on magnetron-sputtered (MS) (11–22) AlN buffers on m-plane sapphire substrates at 1450 °C via hydride vapour phase epitaxy (HVPE). The MS buffers were annealed at high temperatures of 1400–1600 °C. All the samples were characterised using X-ray diffraction, atomic force microscopy, scanning electron microscope and Raman spectrometry. The crystal quality of epilayers regrown by HVPE was improved significantly compared to that of the MS counterpart. With an increasing annealing temperature, the crystal quality of both MS buffers and AlN epilayers measured along [11–23] and [1–100] improved first and then decreased, maybe due to the decomposition of MS buffers, while the corresponding anisotropy along the two directions decreased first and then increased. The optimum quality of the AlN epilayer was obtained at the annealing temperature of around 1500 °C. In addition, it was found that the anisotropy for the epilayers decreased significantly compared to that of annealed MS buffers when the annealing temperature was below 1500 °C.
Applying the Alkali-Activation Method to Encapsulate Silicon Nitride Particles in a Bioactive Matrix for Augmented Strength and Bioactivity
Authors: de la Torre Guido Manuel Olvera , Tatarková Monika , Netriová Zuzana , Barlog Martin , Bertolla Luca , Hnatko Miroslav , Taveri Gianmarco
Published: January 2024

The development of bioactive ceramics still poses challenges in finding a good compromise between bioactivity and mechanical robustness. Moreover, a facile, low-cost and energy-saving synthesis technique is still needed. This study concerns the synthesis of a bioactive material by growing a bioactive Na-Ca-Mg-Si-based ceramic matrix produced using the alkali-activation method on silicon nitride (Si3N4) particles. This technique simultaneously forms the matrix precursor and functionalizes the Si3N4 particles’ surface. The optimal strength–bioactivity compromise was found for the composition containing 60 wt.% Si3N4 and 40 wt.% of the matrix exhibiting good compressive strength of up to 110 MPa and extensive precipitation of hydroxyapatite on the sample surface after 7 days of soaking in simulated body fluid. This innovative approach merging strong non-oxide binary ceramics with the versatile and low-cost alkali-activation method holds great expectations for the future in biomaterials.
Feminization Surgery of the Upper Face as the Crucial Factor in Gender Confirmation—Pearls and Pitfalls
Authors: Pokrowiecki Rafał , Šufliarsky Barbora , Jagielak Maciej
Published: January 2024

Background: Upper-face feminization is a frequently executed procedure in sexual reassignment surgery, owing to its ability to influence gender identity through adjustments to the hairline, forehead, and peri-orbital area. The procedure includes reducing the hairline, lifting the brows, shaving the orbital region, and applying specific techniques to reduce the frontal bone. This research aims to assess the outcomes, results, and potential complications associated with this surgery among transgender patients. Material and Method: Retrospective review of medical records of 20 patients who attended for facial feminization surgery of the upper face between June 2022 and June 2023, analyzing the previously performed procedures, complications and revision surgery outcomes, and first-time procedures. A literature review was performed for similar studies. Results: 20 patients were included in the study. Among the cohort treated elsewhere (n = 11), the primary complaint was insufficient browbone reduction and anterior frontal sinus table setback. They underwent poorly performed Type 1 reduction when full forehead reconstruction (Type 3/4) was indicated (n = 3), or no reduction was performed during hairline advancement (n = 4). Type 3 forehead reduction with orbital shaving and hairline advancement with simultaneous temporal browlift was most commonly performed in both revision and first-time surgical upper face feminization (n = 15) (75%). Type 1 osteoplasty was performed in four patients (10%), one Type 3 revision surgery was performed after insufficient Type 3 reduction, and one case of shock-induced alopecia was reported, treated with PRP/peptides and a FUE hair graft. Conclusions: The author’s preferred technique, ‘whole-in-one’ upper face feminization by modified bi-coronal incision with frontal trichophytic hyper-beveled incision, provides sufficient insight into the frontal bone and orbital region, the desired forehead osteoplasty and the most efficient insight into the temporal area, enabling safe dissection between fasciae, ligamentous adhesion removal, and periosteal attachment, providing full soft and hard tissue feminization. Nevertheless, feminization procedures should be meticulously planned, and all concerning issues should be addressed during the first surgery in order to prevent revisions, complications, and patient dissatisfaction.
A Two-Step Mechanism for Creating Stable, Condensed Chromatin with the Polycomb Complex PRC1
Authors: Seif Elias , Francis Nicole J.
Published: January 2024

The Drosophila PRC1 complex regulates gene expression by modifying histone proteins and chromatin architecture. Two PRC1 subunits, PSC and Ph, are most implicated in chromatin architecture. In vitro, PRC1 compacts chromatin and inhibits transcription and nucleosome remodeling. The long disordered C-terminal region of PSC (PSC-CTR) is important for these activities, while Ph has little effect. In cells, Ph is important for condensate formation, long-range chromatin interactions, and gene regulation, and its polymerizing sterile alpha motif (SAM) is implicated in these activities. In vitro, truncated Ph containing the SAM and two other conserved domains (mini-Ph) undergoes phase separation with chromatin, suggesting a mechanism for SAM-dependent condensate formation in vivo. How the distinct activities of PSC and Ph on chromatin function together in PRC1 is not known. To address this question, we analyzed structures formed with large chromatin templates and PRC1 in vitro. PRC1 bridges chromatin into extensive fibrillar networks. Ph, its SAM, and SAM polymerization activity have little effect on these structures. Instead, the PSC-CTR controls their growth, and is sufficient for their formation. To understand how phase separation driven by Ph SAM intersects with the chromatin bridging activity of the PSC-CTR, we used mini-Ph to form condensates with chromatin and then challenged them with PRC1 lacking Ph (PRC1ΔPh). PRC1ΔPh converts mini-Ph chromatin condensates into clusters of small non-fusing condensates and bridged fibers. These condensates retain a high level of chromatin compaction and do not intermix. Thus, phase separation of chromatin by mini-Ph, followed by the action of the PSC-CTR, creates a unique chromatin organization with regions of high nucleosome density and extraordinary stability. We discuss how this coordinated sequential activity of two proteins found in the same complex may occur and the possible implications of stable chromatin architectures in maintaining transcription states.
Composition of Microbiota in Transient and Mature Human Milk: Significant Changes in Large for Gestational Age Group
Authors: Dinleyici Meltem , Pérez-Brocal Vicente , Arslanoglu Sertac , Aydemir Ozge , Sevuk Ozumut Sibel , Tekin Neslihan , Vandenplas Yvan , Moya Andrés , Dinleyici Ener Cagri
Published: January 2024

The composition of the human milk (HM) microbiota and, consequently, the microorganisms that are passed on to the infant through breastfeeding, can be influenced by various factors such as the mother’s health and diet, gestational age, delivery mode, lactation stage, method of infant feeding, and geographical location. The aim of the Human Milk-Gest Study was to compare the microbiota of transient (postpartum 7–15 days) and mature HM (postpartum 45–90 days) of 44 mothers, and to investigate any potential changes associated with preterm birth, mode of delivery, and birth weight in relation to gestational age. The data were classified into five study groups: normal spontaneous delivery-term (NS-T) newborns, cesarean delivery-term (CS-T) newborns, preterm (PT) newborns (with a gestational age of less than 37 weeks), small for gestational age (SGA) newborns, and large for gestational age (LGA) newborns. An analysis of differential abundance was conducted using ANCOM-BC to compare the microbial genera between transient and mature HM samples as well as between other study groups. A significant difference was detected between HM samples at different sampling times and between the study groups (p < 0.01). In transient HM samples, Ralstonia, Burkholderiaceae_uc, and Pelomonas were significantly dominant in the LGA group compared to the NS-T, CS-T, PT, and SGA groups. In mature HM samples, Burkholderiaceae_uc, Ralstonia, Pelomonas, and Klebsiella were significantly dominant in the LGA group compared to the NS-T, CS-T, and PT groups, while Ralstonia, Burkholderiaceae_uc, and Pelomonas were significantly dominant in the LGA group compared to the SGA group. Differences were also detected between the transient and mature HM samples in the CS-T, PT, SGA, and LGA groups, but no differences occurred in the NS-T groups. In conclusion, we showed that Ralstonia, Burkholderiaceae_uc, and Pelomonas were significantly dominant in the LGA group in transient HM and continued in mature HM. The body mass index (BMI) of the mothers in the LGA group was not >30 at conception, however, the maternal BMI at birth and maternal weight gain during pregnancy were higher than in the other groups. The nutritional composition of HM is specifically designed to meet infant nutritional requirements during early life. Evaluating the effects of HM microbiota on infant microbiota composition and short- and long-term health effects in larger studies would be useful.
Impact of Vitamin D on Osseointegration in Dental Implants: A Systematic Review of Human Studies
Authors: Buzatu Berivan Laura Rebeca , Buzatu Roxana , Luca Magda Mihaela
Published: January 2024

This systematic review evaluates the impact of Vitamin D levels on dental implant osseointegration, hypothesizing that optimal Vitamin D enhances success rates, and aims to synthesize data on its relationship with clinical outcomes in implantology. A comprehensive search across PubMed, Cochrane Library, and Web of Science databases included seven peer-reviewed articles meeting the criteria for the review. These studies, conducted between 2008 and 2021, included human subjects and explicitly correlated serum Vitamin D levels with dental implant outcomes, following PRISMA guidelines. The selected studies involved 1462 participants and examined 4450 dental implants. Key findings included a varied implant loss rate ranging from 3.9% to 11.4% across the studies. One study reported a 9.8% implant loss rate, yet found no significant association between Vitamin D receptor polymorphism and implant success. Another study indicated successful implantation following Vitamin D3 supplementation, even in severe deficiency cases. The highest implant loss rate (11.1%) was observed in severely Vitamin D-deficient patients, particularly when compounded by risk factors such as smoking and periodontal disease. Additionally, one study noted significantly improved bone density following post-surgical Vitamin D supplementation for up to 12 weeks. The review supports a link between sufficient Vitamin D levels and successful dental implant osseointegration, suggesting Vitamin D deficiency as a potential risk factor for increased failure and advocating for Vitamin D evaluations in pre-surgical planning to potentially enhance implantology outcomes.
Anti-Wrinkle and Skin Moisture Efficacy of 7-MEGATM: A Randomized, Double-Blind, Placebo Comparative Clinical Trial
Authors: Sung Hyun Kyung , Kim Tae Jeong , Kim Hyung Mook , Youn Sang Jun , Choi Yong , Lee Na Young , Oh Hyun Jeong , Kwon Hyuck Se , Shin Seon Mi
Published: January 2024

7-MEGATM is a food product made from purified Alaska pollack fish oil containing palmitoleic acid (16:1), commonly referred to as omega-7. We sought to quantitatively evaluate whether this substance inhibits skin aging. A total of 101 middle-aged females were randomly allocated to the intervention (N = 50) or placebo group (N = 51). Each participant was advised to take either 500 mg of 7-MEGATM or a placebo twice daily for 12 weeks. The primary outcomes were the degree of improvement in wrinkles and the degree of moisture filling after consumption for 12 weeks compared to baseline. The secondary outcomes were improvement in skin wrinkles; moisture changes at 4 and 8 weeks from baseline; changes in transdermal water loss, skin elasticity, the melanin index, the erythema index, and the Global Photo Damage Score. We found a significant improvement in skin wrinkles and elasticity at 12 weeks in the 7-MEGATM-consuming group compared to that in the placebo group; skin moisture, elasticity, and the melanin index were also improved. No supplement-related adverse reactions were observed and 7-MEGATM was identified as safe. 7-MEGATM was effective for human skin function in terms of wrinkles, moisture, elasticity, and melanin production and may be useful as a skin nutritional supplement.
Analysis of Dietary Patterns Associated with Kidney Stone Disease Based on Data-Driven Approaches: A Case-Control Study in Shanghai
Authors: Wang Yifei , Liu Shaojie , Zhao Qi , Wang Na , Liu Xing , Zhang Tiejun , He Gengsheng , Zhao Genming , Jiang Yonggen , Chen Bo
Published: January 2024

The main objective of this study was to analyze dietary patterns using data-driven approaches and to explore preventive or risk dietary factors for kidney stone disease (KSD). A case–control matching study was conducted in adults (n = 6396) from a suburb of Shanghai. A food frequency questionnaire was used to assess the consumption of various types of food, and B-ultrasound was used to identify kidney stones. Principal component analysis and regression were used to generate dietary patterns and further explore the relationship between dietary patterns and KSD. LASSO regression and post-selection inference were used to identify food groups most associated with KSD. Among males, the “balanced but no-sugary-beverages pattern” (OR = 0.78, p < 0.05) and the “nuts and pickles pattern” (OR = 0.84, p < 0.05) were protective dietary patterns. Among females, “high vegetables and low-sugary-beverages pattern” (OR = 0.83, p < 0.05) and “high-crustaceans and low-vegetables pattern” (OR = 0.79, p < 0.05) were protective dietary patterns, while the “comprehensive pattern with a preference for meat” (OR = 1.06, p < 0.05) and “sugary beverages pattern” (OR = 1.16, p < 0.05) were risk dietary patterns. We further inferred that sugary beverages (p < 0.05) were risk factors and pickles (p < 0.05) and crustaceans (p < 0.05) were protective factors.
Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa
Authors: Kolo Francis B. , Adesiyun Abiodun A. , Fasina Folorunso O. , Harris Bernice N. , Rossouw Jennifer , Byaruhanga Charles , Geyer Hermanus De Wet , Blumberg Lucille , Frean John , van Heerden Henriette
Published: January 2024

Abattoir workers are liable to zoonotic infections from animals and animal products, primarily to diseases with asymptomatic and chronic clinical manifestations in animals, such as brucellosis. No published reports exist on the seroprevalence of brucellosis in abattoir workers in South Africa. Therefore, this cross-sectional study was conducted to estimate the occurrence and risk factors for Brucella exposure in abattoir workers in Gauteng Province. A total of 103 abattoir workers and managers from 6 abattoirs, where brucellosis-positive slaughtered cattle and sheep were previously detected, were interviewed and tested with serological assays using the Rose Bengal test (RBT), BrucellaCapt, and IgG-ELISA. A pre-tested questionnaire was administered to consenting respondents to obtain information on risk factors for brucellosis. Of the 103 respondents tested, the distribution of female and male workers was 16 (15.5%) and 87 (84.5%), respectively. The seroprevalence for exposure to brucellosis was 21/103 (20.4%, 95%CI: 13.1–29.5) using a combination of RBT, BrucellaCapt, or IgG-ELISA. For test-specific results, seroprevalences by RBT, BrucellaCapt, and IgG-ELISA were 13/103 (12.6%, 95%CI: 6.9–20.6), 9/103 (8.74%, 95%CI: 4.1–15.9), and 18/103 (17.5%, 95%CI: 10.7–26.2), respectively. Low-throughput abattoirs were identified as associated risks, as 29.3% of workers were seropositive compared with 12.7% of workers in high-throughput abattoirs, which highlights that direct contact at abattoirs poses higher risk to workers than indirect and direct contact outside abattoirs. This study confirms the occurrence of Brucella spp. antibodies among abattoir workers in South Africa, possibly due to occupational exposure to Brucella spp., and highlights the occupational hazard to workers. Furthermore, findings underscore that abattoir facilities can serve as points for active and passive surveillance for indicators of diseases of public health importance. We recommend periodic implementation of brucellosis testing of abattoir workers country-wide to establish baseline data for informing appropriate preventive practices and reducing the potential burden of infection rates among these high-risk workers.
Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from Pectis brevipedunculata (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems
Authors: Lopes Auxiliadora Cristina Correa Barata , do Nascimento Jessyane Rodrigues , Camara Marcos Bispo Pinheiro , Lima Aldilene da Silva , Lopes Gláucia Laís Nunes , do Nascimento Matheus Oliveira , Xavier Júlia Karla Albuquerque Melo , de Jesus Caroline Martins , Mendonça Cáritas de Jesus Silva , Carvalho André Luis Menezes , Silva Lucilene Amorim , da Rocha Cláudia Quintino
Published: January 2024

Pectis brevipedunculata (Gardner) Sch.Bip., known in Brazil as alecrim do campo, is a small Asteraceae family plant with a calming effect and consumed as tea. This species contains components, such as neral and geranial, that display various biological activities, such as leishmanicidal. The aim was to chemically characterize the essential oil (EO) obtained from P. brevipedunculata (EO-PB) by hydrodistillation and a microemulsion formulated with EO (ME-PB), Tween 80 and Transcutol P, assess the leishmanicidal effect against Leishmania (L.) amazonensis promastigotes and cytotoxicity against RAW 264.7. EO-PB and ME-PB were analyzed by Gas Chromatography Mass Spectrometry (GC/MS). Monoterpene hydrocarbons were noteworthy among the identified compounds. The main EO-PB constituents were α-pinene and limonene, followed by neral and geranial, which were maintained in ME-PB. EO-PB presented an inhibitory concentration (IC50) of 20 µg/mL and ME-PB of 0.93 µg/mL. ME-PB inhibition towards the parasite was 20-fold higher than that of EO-PB. This indicated that EO incorporation to the microemulsion resulted in optimized biological activity. Selectivity indices indicate that ME-PB is more selective concerning parasite inhibition. Thus, ME-PB may comprise an adequate approach against Leishmania, as the inhibitory concentration (IC50) promastigotes was lower than that considered toxic for cells cell cytotoxicity of 50% (CC50).