bluuebunny/crossref_metadata_2025 huggingface dataset:
TCR signaling and cellular metabolism regulate the capacity of murine epidermal γδ T cells to rapidly produce IL-13 but not IFN-γ
Authors: Ibusuki Atsuko , Kawai Kazuhiro , Nitahara-Takeuchi Ayano , Argüello Rafael J. , Kanekura Takuro
Published: February 2024

Resident epidermal T cells of murine skin, called dendritic epidermal T cells (DETCs), express an invariant γδ TCR that recognizes an unidentified self-ligand expressed on epidermal keratinocytes. Although their fetal thymic precursors are preprogrammed to produce IFN-γ, DETCs in the adult epidermis rapidly produce IL-13 but not IFN-γ early after activation. Here, we show that preprogrammed IFN-γ-producing DETC precursors differentiate into rapid IL-13 producers in the perinatal epidermis. The addition of various inhibitors of signaling pathways downstream of TCR to the in vitro differentiation model of neonatal DETCs revealed that TCR signaling through the p38 MAPK pathway is essential for the functional differentiation of neonatal DETCs. Constitutive TCR signaling at steady state was also shown to be needed for the maintenance of the rapid IL-13-producing capacity of adult DETCs because in vivo treatment with the p38 MAPK inhibitor decreased adult DETCs with the rapid IL-13-producing capacity. Adult DETCs under steady-state conditions had lower glycolytic capacity than proliferating neonatal DETCs. TCR stimulation of adult DETCs induced high glycolytic capacity and IFN-γ production during the late phase of activation. Inhibition of glycolysis decreased IFN-γ but not IL-13 production by adult DETCs during the late phase of activation. These results demonstrate that TCR signaling promotes the differentiation of IL-13-producing DETCs in the perinatal epidermis and is needed for maintaining the rapid IL-13-producing capacity of adult DETCs. The low glycolytic capacity of adult DETCs at steady state also regulates the rapid IL-13 response and delayed IFN-γ production after activation.
The genomic study of repetitive elements in Solea senegalensis reveals multiple impacts of transposable elements in the evolution and architecture of Pleuronectiformes chromosomes
Authors: Cross Ismael , Rodríguez María E. , Portela-Bens Silvia , Merlo Manuel A. , Gálvez-Salido Aaron , Navajas-Pérez Rafael , Rebordinos Laureana
Published: February 2024

Pleuronectiformes are flatfishes with high commercial value and a prominent example of successful marine adaptation through chromosomal evolution. Hence, the aim of this study was to analyze the 14 relative abundance of repetitive elements (satellite DNA and transposable elements (TE)) in the 15 genome of 10 fish species (8 flatfish) delving into the study of the species of special relevance, 16 Senegalese sole, Solea senegalensis. The results showed differences in the abundance of repetitive elements, with S. senegalensis exhibiting the highest frequency and coverage of these elements reaching the 40% of the genome and not at random distribution. It is noteworthy the presence of relevant peaks of Helitrons in centromeric/pericentromeric positions mainly in the bi-armed chromosomes 1, 2, 4, 6, 7, and 9. The position of the centromeres of this species determined through the genomic localization of the family of satellite DNA PvuII, and other repetitive sequences was obtained de novo. This allowed us to know the genomic position of the centromeres in 19 out of the 21 chromosomes of S. senegalensis. Helitrons showed an accumulation of tandem copies mainly in the pericentromeric positions of chromosomes 1 and 2, occupying a region, in the first case, of 600Kb of tandem repeats. That has only been previously described in mammals and plants. Divergence and copy number studies indicated the presence of active families in the species’ genome and the existence of two important events of transposon activity (burst) in the genome of S. senegalensis, mainly accentuated in Helitrons. The results showed that only the families of DNA transposons exhibited a landscape with symmetrical bell-shaped distribution. The phylogenetic analysis of Helitron families revealed the presence of two large groups of families and the presence of four groups of sequences with heterogeneous distribution among chromosomes. Finally, the phylogenomic analysis of 8615 sequences belonging to Helitron insertions from 5 families of flatfish and two external species, allowed to classify the copies into nine groups of sequences with different levels of divergence and clusters, including some branches with distant phylogenetically species. The implications of this study will help to expand the knowledge of chromosome structure and evolution of these species.
Pharmacological interventions for the treatment of obstructive sleep apnea syndrome
Authors: Liu Jin , Yang Xiaolan , Li Guangcai , Liu Peijun
Published: March 2024

Obstructive Sleep Apnea Syndrome (OSAS) affects 13–33% of males and 6–9% of females globally and poses significant treatment challenges, including poor adherence to Continuous Positive Airway Pressure (CPAP) and residual excessive sleepiness (RES). This review aims to elucidate the emerging interest in pharmacological treatments for OSAS, focusing on recent advancements in this area. A thorough analysis of extensive clinical trials involving various drugs, including selective dopamine reuptake inhibitors, selective norepinephrine inhibitors, combined antimuscarinic agents, and orexin agonists, was conducted. These trials focused on ameliorating respiratory metrics and enhancing sleep quality in individuals affected by OSAS. The studied pharmacological agents showed potential in improving primary outcomes, notably the apnea-hypopnea index (AHI) and the Epworth sleepiness scale (ESS). These improvements suggest enhanced sleep quality and symptom management in OSAS patients. With a deeper understanding of OSAS, pharmacological interventions are emerging as a promising direction for its effective management. This review provides a comprehensive overview of the current state of drug research in OSAS, highlighting the potential of these treatments in addressing the disorder’s complex challenges.
Antibacterial and antibiofilm activities of protocatechualdehyde and its synergy with ampicillin against methicillin-resistant Staphylococcus aureus
Authors: Wang Ying , Liu Xiaojing , Song Lin , Chen Kun , Shi Chao , Peng Chuantao , Yang Qingli , Li Zhaojie
Published: February 2024

Protocatechualdehyde (PA) is a phenolic acid present in many plants and has many biological activities. Herein, the antagonistic effects and the action mechanism of PA against methicillin-resistant Staphylococcus aureus (MRSA) were studied. The results showed that PA had both significant antibacterial and anti-biofilm activities against MRSA. Additionally, PA had synergy with ampicillin against MRSA. It was elucidated that PA was prominent in destroying cell membranes, increasing cell membrane permeability and intracellular ROS production, thus leading to bacterial cell damage. Transcriptome analysis showed that PA disrupts many physiological pathways, including increasing cell membrane permeability, inhibiting biofilm formation, decreasing resistance to antimicrobial agents, and impairing DNA replication. Finally, the antimicrobial preservation test showed that PA could inhibit the growth of MRSA and prevent the corruption of beef. In summary, PA is an effective natural antibacterial substance and has a good application potential in food preservation, even in tackling antibiotic resistance problems.
A case report of a patient with recurrent and severe infections highlighting the importance of considering inborn errors of immunity
Authors: Altammar Fajer , Alshamali Mohammed , Alqunaee Marwan , Alali Ahmad J. , Elshafie Reem M. , Al-Herz Waleed
Published: February 2024

Inborn errors of immunity (IEI) can often be misdiagnosed early in life due to their heterogenous clinical presentations. Interleukin-1 receptor-associated kinase 4 (IRAK-4) deficiency is one of the rare innate immunodeficiency disorders. We present the case of a patient who presented at the age of 15 days with meningitis and septic shock that responded to antibiotics. She was admitted again at the age of 45 days with pseudomonas aeruginosa bacteremia that was associated with increased inflammatory markers. Her third admission was at the age of 2.5 months due to left sided peri-orbital cellulitis that was again associated with elevated inflammatory markers. At 3.5 months, she experienced left orbital cellulitis, which was complicated by extensive sinus involvement, erosion, and abscess formation in the pterygopalatine fossa. Her condition progressed to septic shock and required multiple antibiotics and surgical interventions for drainage and control of the infection source. Both abscess and blood culture were positive for pseudomonas aeruginosa. An IEI was suspected but basic immunology testing was normal. Whole Exome Sequencing was performed and a novel mutation in IRAK4 was detected. In conclusion, we highlight the importance of raising awareness among pediatricians about the potentially lethal IEI and the need to consult specialists when these diseases are suspected. Among them is IRAK-4 deficiency which can be diagnosed by sophisticated functional assays and/or genetic testing.
Feasibility study on the introduction of Micro-CT technology for the identification of Radix Bupleuri and its adulterants
Authors: Chen Kehong , Chen Gong , Zhuang Zhelong , Luo Shouhua , Liu Jiaming , Liu Guorong
Published: February 2024

Background:Radix Bupleuri, a kind of Chinese herbal medicine with great clinical use, is often confused with its adulterants, and it is difficult to identify it without certain knowledge. The existing identification methods have their own drawbacks, so a new method is needed to realize the identification of Radix Bupleuri and its adulterants.

Methods: We used Micro Computed Tomography (Micro-CT) to perform tomography scans on Radix Bupleuri and its adulterants, performed data screening and data correction on the obtained DICOM images, and then applied 3D reconstruction, data augmentation, and ResNext deep learning model for the classification study.

Results: The DICOM images after data screening, data correction, and 3D reconstruction can observe the differences in the microstructure of Radix Bupleuri and its adulterants, thus enabling effective classification and analysis. Meanwhile, the accuracy of classification using the ResNext model reached 75%.

Conclusion: The results of this study showed that Micro-CT technology is feasible for the authentication of Radix Bupleuri. The pre-processed and 3D reconstructed tomographic images clearly show the microstructure and the difference between Radix Bupleuri and its adulterants without damaging the internal structure of the samples. This study concludes that Micro-CT technology provides important technical support for the reliable identification of Radix Bupleuri and its adulterants, which is expected to play an important role in the quality control and clinical application of herbs.
Ac-SDKP attenuates ER stress-stimulated collagen production in cardiac fibroblasts by inhibiting CHOP-mediated NF-κB expression
Authors: Suhail Hamid , Peng Hongmei , Matrougui Khalid , Rhaleb Nour-Eddine
Published: March 2024

Inflammation and cardiac fibrosis are prevalent pathophysiologic conditions associated with hypertension, cardiac remodeling, and heart failure. Endoplasmic reticulum (ER) stress triggers the cells to activate unfolded protein responses (UPRs) and upregulate the ER stress chaperon, enzymes, and downstream transcription factors to restore normal ER function. The mechanisms that link ER stress-induced UPRs upregulation and NF-κB activation that results in cardiac inflammation and collagen production remain elusive. N-Acetyl-Ser-Asp-Lys-Pro (Ac-SDKP), a natural tetrapeptide that negatively regulates inflammation and fibrosis, has been reported. Whether it can inhibit ER stress-induced collagen production in cardiac fibroblasts remains unclear. Thus, we hypothesized that Ac-SDKP attenuates ER stress-stimulated collagen production in cardiac fibroblasts by inhibiting CHOP-mediated NF-κB expression. We aimed to study whether Ac-SDKP inhibits tunicamycin (TM)-induced ER stress signaling, NF-κB signaling, the release of inflammatory cytokine interleukin-6, and collagen production in human cardiac fibroblasts (HCFs). HCFs were pre-treated with Ac-SDKP (10 nM) and then stimulated with TM (0.25 μg/mL). We found that Ac-SDKP inhibits TM-induced collagen production by attenuating ER stress-induced UPRs upregulation and CHOP/NF-κB transcriptional signaling pathways. CHOP deletion by specific shRNA maintains the inhibitory effect of Ac-SDKP on NF-κB and type-1 collagen (Col-1) expression at both protein and mRNA levels. Attenuating ER stress-induced UPR sensor signaling by Ac-SDKP seems a promising therapeutic strategy to combat detrimental cardiac inflammation and fibrosis.
Deficits in proprioception and strength may contribute to the impaired postural stability among individuals with functional ankle instability
Authors: Liu Yanhao , Dong Shiyu , Wang Qi , Liu Ziyin , Song Qipeng , Shen Peixin
Published: March 2024

Purpose: The correlations of postural stability with proprioception and strength may explain the recurrent sprains among individuals with functional ankle instability (FAI). This study aimed to compare anterior-posterior (AP) and medial-lateral (ML) postural stability, along with ankle proprioception and strength between individuals with and without FAI and investigated their correlations.

Methods: Forty participants with FAI and another 40 without FAI were recruited. Their postural stability, represented by time to stabilization (TTS) in the AP (TTSAP) and ML (TTSML) directions, was calculated by the ground reaction force during jumping onto a force plate. Their ankle proprioception and strength during plantarflexion/dorsiflexion and inversion/eversion were measured using a proprioception device and a strength testing system, separately.

Results: Individuals with FAI had longer TTSAP (p = 0.015) and TTSML (p = 0.006), larger ankle proprioception thresholds (p = 0.000–0.001), and less strength (p = 0.001–0.017) than those without FAI. Correlations between strength and TTSAP were detected among individuals with (ankle plantarflexion, r = −0.409, p = 0.009) and without FAI (ankle plantarflexion, r = −0.348, p = 0.028; ankle dorsiflexion, r = −0.473, p = 0.002). Correlations of proprioception (ankle inversion, r = 0.327, p = 0.040; ankle eversion, r = 0.354, p = 0.025) and strength (ankle eversion, r = −0.479, p = 0.002) with TTSML were detected among individuals without FAI but not among those with FAI.

Conclusion: Individuals with FAI have worse postural stability and proprioception and less strength. Their proprioception and strength decreased to a point where they could not provide sufficient functional assistance to the ML postural stability. Improvements in proprioception and strength may be keys to prevent recurrent ankle sprains among individuals with FAI.
Deep learning for automated segmentation and counting of hypocotyl and cotyledon regions in mature Pinus radiata D. Don. somatic embryo images
Authors: Davidson Sam J. , Saggese Taryn , Krajňáková Jana
Published: March 2024

In commercial forestry and large-scale plant propagation, the utilization of artificial intelligence techniques for automated somatic embryo analysis has emerged as a highly valuable tool. Notably, image segmentation plays a key role in the automated assessment of mature somatic embryos. However, to date, the application of Convolutional Neural Networks (CNNs) for segmentation of mature somatic embryos remains unexplored. In this study, we present a novel application of CNNs for delineating mature somatic conifer embryos from background and residual proliferating embryogenic tissue and differentiating various morphological regions within the embryos. A semantic segmentation CNN was trained to assign pixels to cotyledon, hypocotyl, and background regions, while an instance segmentation network was trained to detect individual cotyledons for automated counting. The main dataset comprised 275 high-resolution microscopic images of mature Pinus radiata somatic embryos, with 42 images reserved for testing and validation sets. The evaluation of different segmentation methods revealed that semantic segmentation achieved the highest performance averaged across classes, achieving F1 scores of 0.929 and 0.932, with IoU scores of 0.867 and 0.872 for the cotyledon and hypocotyl regions respectively. The instance segmentation approach demonstrated proficiency in accurate detection and counting of the number of cotyledons, as indicated by a mean squared error (MSE) of 0.79 and mean absolute error (MAE) of 0.60. The findings highlight the efficacy of neural network-based methods in accurately segmenting somatic embryos and delineating individual morphological parts, providing additional information compared to previous segmentation techniques. This opens avenues for further analysis, including quantification of morphological characteristics in each region, enabling the identification of features of desirable embryos in large-scale production systems. These advancements contribute to the improvement of automated somatic embryogenesis systems, facilitating efficient and reliable plant propagation for commercial forestry applications.
MANdatory - why men need (and are needed for) gender equality progress
Authors: Van Laar Colette , Van Rossum Aster , Kosakowska-Berezecka Natasza , Bongiorno Renata , Block Katharina
Published: March 2024

While much progress has been made towards gender equality, diversity and inclusion in the workplace, education and society, recent years have also revealed continuing challenges that slow or halt this progress. To date, the majority of gender equality action has tended to approach gender equality from one side: being focused on the need to remove barriers for girls and women. We argue that this is only half the battle, and that a focus on men is MANdatory, highlighting three key areas: First, we review men’s privileged status as being potentially threatened by progress in gender equality, and the effects of these threats for how men engage in gender-equality progress. Second, we highlight how men themselves are victims of restrictive gender roles, and the consequences of this for men’s physical and mental health, and for their engagement at work and at home. Third, we review the role of men as allies in the fight for gender equality, and on the factors that impede and may aid in increasing men’s involvement. We end with recommendations for work organizations, educational institutions and society at large to reach and involve men as positive agents of social change.
Evaluation of daytime sleepiness and insomnia symptoms in OSA patients with a characterization of symptom-defined phenotypes and their involvement in depression comorbidity—a cross-sectional clinical study
Authors: Gabryelska Agata , Turkiewicz Szymon , Białasiewicz Piotr , Grzybowski Filip , Strzelecki Dominik , Sochal Marcin
Published: March 2024

Introduction

Recent research highlights the significance of insomnia and sleepiness, shifting from obstructive sleep apnea (OSA) severity and sleep structure, in defining OSA phenotypes.

Objectives

This study aimed to characterize insomnia and sleepiness associated with OSA phenotypes and assess their involvement in depression symptoms (DS) in OSA.

Materials and methods

This cross-sectional, clinical study included 181 participants who underwent polysomnography (PSG) and filled out questionnaires, including the Epworth Sleepiness Scale (ESS), Insomnia Severity Index (ISI), Pittsburgh Sleep Quality Index (PSQI), and Beck Depression Index (BDI). They were categorized into phenotypes: insomnia–sleepiness (I + S; ESS ≥ 11; ISI ≥ 15; n = 20), sleepiness (S; ESS ≥ 11; ISI < 15; n = 22), insomnia (I; ESS < 11; ISI ≥ 15), and asymptomatic (A; ESS < 11; ISI<15; n=55).

Results

A linear regression model for the BDI score (R2 = 0.357, p < 0.001) included ISI score and subjective-to-objective sleep latency ratio. The ISI score was a predictive factor for mild and moderate DS [OR = 1.23 (95% CI: 1.09–1.38), p < 0.001 and OR = 1.39 (95% CI: 1.13–1.72), p = 0.002]. The I and I + S phenotypes are characterized by higher BDI scores (p < 0.001 and p = 0.02), longer subjective sleep latency (p = 0.008 and p = 0.04), and shorter subjective total sleep time (TST; p = 0.049 and p = 0.006) compared to A. Furthermore, the I and I + S groups had shorter subjective TST than S (p = 0.03 and p = 0.047). The I and I + S had higher BDI scores than A (p < 0.001 and p = 0.02, respectively) and S (p < 0.001 and p = 0.02, respectively). The I phenotype was associated with the risk of mild and moderate DS (OR = 5.61 (95% CI: 1.91–16.53), p < 0.001 and OR = 9.55 (95% CI: 1.81–50.48), p = 0.008 respectively). Moreover, the I + S phenotype presented an even greater risk for mild DS (OR = 10.29 (95% CI: 2.95–35.85), p < 0.001).

Conclusion

Using clinical features for OSA phenotyping holds promise for finding OSA individuals with increased risk for DS occurrence.
Attitudes toward COVID-19 vaccines during pregnancy and breastfeeding
Authors: Simsekoglu Nesibe , Akyuz Enes , Guven Rabia , Pasin Ozge
Published: March 2024

Background

Although vaccination is one of the most effective means of controlling the spread of COVID-19, public concerns and indecision about vaccination still continue. Because pregnant and breastfeeding individuals are at high risk for severe outcomes in case of infections, determining their level of hesitation and attitude toward COVID-19 vaccines will guide the management of the disease. This study aimed to determine pregnant and breastfeeding women’s levels of hesitation and attitude toward COVID-19 vaccines as well as their related factors.

Methods

The sample of this descriptive research consisted of 103 pregnant or breastfeeding individuals who were seen at the obstetrics and gynecology outpatients clinic of a state hospital in Istanbul, Turkey. The data were collected using a ‘demographic data form’, the ‘Vaccine Hesitancy Scale in Pandemic’, and the ‘Attitudes toward COVID-19 Vaccine Scale’. The research data were analyzed with appropriate statistical methods.

Results

The mean age of the participants was 29.71 ± 4.75, 51% were pregnant, and 74.8% had received the COVID-19 vaccine. The mean score of the ‘Vaccination Hesitancy Scale in Pandemic’ was 30.83 ± 6.91, and the mean score for the ‘Attitude Scale toward the COVID-19 Vaccine’ was 25.50 ± 5.20. A significant difference was found between the total score of the ‘Vaccine Hesitation Scale in the Pandemic’ and the mean score of the ‘Lack of Confidence’ sub-dimension between the ‘working status’ and the ‘influenza vaccination’ status. In terms of the mean score of the ‘Risk’ sub-dimension, a significant difference was found between the ‘period of vaccination’ (p < 0.05). According to the mean total score of the ‘Attitude Towards COVID-19 Vaccine Scale’, there was a significant difference between the ‘smoking’ status. There was a significant difference in the ‘Positive Attitude’ sub-dimension in terms of the ‘flu vaccination’ status. There was a significant difference in the ‘Negative Attitude’ sub-dimension in terms of the ‘chronic disease’ status. A positive correlation was found between the total scores of the scales.

Conclusion

It was concluded that although the participants had a high level of hesitation toward the COVID-19 vaccine, they had a positive attitude. The results obtained will be guided in determining the strategies to be developed for these specific groups in future pandemics.
Prevalence and risk factors of musculoskeletal injuries in modern and contemporary dancers: a systematic review and meta-analysis
Authors: Sun Yufei , Liu Hui
Published: February 2024

Background

A large number of studies have found that the musculoskeletal injury of modern and contemporary dancers has a high incidence. Previous publications have indicated that there are many potential factors that are related to dancing injury; however, they have not been proven, and even some data are conflicting in different research.

Results

The search yielded 18 prospective studies reporting on professional and pre-professional modern or contemporary dancers from companies and schools. The prevalence of modern and contemporary dancers was 0.82 (95% CI: 0.74~0.90). The injury proportion of trauma, overuse, ankle and foot, lower extremity, joint and ligaments, muscle and tendons, and time-loss were 0.40, 0.26, 0.49, 0.34, 0.33, and 0.29 in the total number of injuries. There was no evidence of a significant difference in sex, age, and education program. The factors of BMI and injury history achieved statistical significance, and the p-values were less than 0.01.

Conclusion

Based on the results of this article, BMI and injury history may be risk factors for injury in contemporary and modern dancers. Modern and contemporary dancers have a high prevalence of musculoskeletal injuries. Lower extremity injuries are the most common in the whole body, with injuries to the foot and ankle being more frequent. The mechanism of injury is mostly overuse injury, and the injured tissues are mostly muscle tendons and joint ligaments.
Ascertaining injury risk issues through big data analysis: text-mining based analysis of national emergency response data
Authors: Won Jin-Young , Lee Yu-Rim , Cho Myeong-Heum , Kim Yun-Tae , Lee Ji-Hyang
Published: February 2024

Objectives

Injury prevention can be achieved through various interventions, but it faces challenges due to its comprehensive nature and susceptibility to external environmental factors, making it difficult to detect risk signals. Moreover, the reliance on standardized systems leads to the construction and statistical analysis of numerous injury surveillance data, resulting in significant temporal delays before being utilized in policy formulation. This study was conducted to quickly identify substantive injury risk problems by employing text mining analysis on national emergency response data, which have been underutilized so far.

Methods

With emerging issue and topic analyses, commonly used in science and technology, we detected problematic situations and signs by deriving injury keywords and analyzing time-series changes.

Results

In total, 65 injury keywords were identified, categorized into hazardous, noteworthy, and diffusion accidents. Semantic network analysis on hazardous accident terms refined the injury risk issues.

Conclusion

An increased risk of winter epidemic fractures due to extreme weather, self-harm due to depression (especially drug overdose and self-mutilation), and falls was observed in older adults. Thus, establishing effective injury prevention strategies through inter-ministerial and interagency cooperation is necessary.
Diet quality and snack preferences of Turkish adolescents in private and public schools
Authors: Sezer Fatma Elif , Alpat Yavaş İdil , Saleki Neda , Bakırhan Hande , Pehlivan Merve
Published: March 2024

Introduction

Socioeconomic level is one of the important factors determining diet quality. Snack preferences are affected by socioeconomic level. The objective of this research was to determine the effect of socioeconomic levels on diet quality and snack preferences among adolescents from different socioeconomic backgrounds.

Methods

The study involved 118 adolescents aged between 10-18 years residing in Istanbul. A questionnaire prepared by the researchers was used to obtain information on the adolescents’ dietary habits, consumption of main meals and snacks, habits, and food consumption records. The participants’ food consumption was assessed using the retrospective 24-hour recall method, and diet quality was evaluated using the calculated nutrient adequacy ratio (NAR) and mean adequacy ratio (MAR).

Results

The mean age of the adolescents was 16.42±0.89 years. The number of snacks consumed in private schools was found to be higher than in public schools (p < 0.05). The NAR score for vitamin C consumption was significantly higher in private schools compared to public schools (p < 0.05). Although the MAR scores of adolescents in private schools were higher than those in public schools, this difference was not statistically significant. The majority of adolescents in private schools regularly consumed fresh fruit (67.2%), milk (60.3%), yogurt (60.3%), and nuts (56.9%) as snacks. In contrast, 45% of adolescents in public schools regularly consumed pastries (p < 0.05).

Discussion

It was observed that adolescents studying in public schools had a lower tendency to prefer healthy foods for snacks compared to those in private schools. Socioeconomic level was identified as an important factor influencing eating habits during adolescence. Considering that the level of income is significantly different between the adolescents studying at private and public schools, the higher consumption of snacks by the adolescents studying at private school may be associated with higher income.
Low-profile prosthetic foot stiffness category and size, and shoes affect axial and torsional stiffness and hysteresis
Authors: Tacca Joshua R. , Colvin Zane A. , Grabowski Alena M.
Published: February 2024

Introduction

Passive-elastic prosthetic feet are manufactured with numerical stiffness categories and prescribed based on the user's body mass and activity level, but mechanical properties, such as stiffness values and hysteresis are not typically reported. Since the mechanical properties of passive-elastic prosthetic feet and footwear can affect walking biomechanics of people with transtibial or transfemoral amputation, characterizing these properties can provide objective metrics for comparison and aid prosthetic foot prescription and design

Methods

We characterized axial and torsional stiffness values, and hysteresis of 33 categories and sizes of a commercially available passive-elastic prosthetic foot model [Össur low-profile (LP) Vari-flex] with and without a shoe. We assumed a greater numerical stiffness category would result in greater axial and torsional stiffness values but would not affect hysteresis. We hypothesized that a greater prosthetic foot length would not affect axial stiffness values or hysteresis but would result in greater torsional stiffness values. We also hypothesized that including a shoe would result in decreased axial and torsional stiffness values and greater hysteresis.

Results

Prosthetic stiffness was better described by curvilinear than linear equations such that stiffness values increased with greater loads. In general, a greater numerical stiffness category resulted in increased heel, midfoot, and forefoot axial stiffness values, increased plantarflexion and dorsiflexion torsional stiffness values, and decreased heel, midfoot, and forefoot hysteresis. Moreover, for a given category, a longer prosthetic foot size resulted in decreased heel, midfoot, and forefoot axial stiffness values, increased plantarflexion and dorsiflexion torsional stiffness values, and decreased heel and midfoot hysteresis. In addition, adding a shoe to the prosthetic foot resulted in decreased heel and midfoot axial stiffness values, decreased plantarflexion torsional stiffness values, and increased heel, midfoot, and forefoot hysteresis.

Discussion

Our results suggest that manufacturers should adjust the design of each category to ensure the mechanical properties are consistent across different sizes and highlight the need for prosthetists and researchers to consider the effects of shoes in combination with prostheses. Our results can be used to objectively compare the LP Vari-flex prosthetic foot to other prosthetic feet to inform their prescription, design, and use for people with a transtibial or transfemoral amputation.
Challenges in the delivery of health services for people living with HIV in Dar es Salaam, Tanzania: a qualitative descriptive study among healthcare providers
Authors: Iseselo Masunga K. , Ambikile Joel S. , Lukumay Gift G. , Mosha Idda H.
Published: February 2024

Background

Healthcare providers play an important role in the provision of health services in care and treatment clinics (CTCs), as they help clients cope with their HIV/AIDS diseases by providing health education and counseling. Little is known about the challenges that healthcare providers face when providing such services to people living with HIV (PLWH) in low-resource settings. This study aimed to explore and understand the challenges that healthcare providers face in delivering care to PLWH in Dar es Salaam, Tanzania.

Materials and methods

We conducted a qualitative descriptive study to explore and understand the challenges that healthcare providers face when providing health services to PLWH. This study was carried out in the CTCs in the Ubungo District, Dar es Salaam, Tanzania. A total of 10 healthcare providers were recruited using a purposive sampling technique until information saturation was attained. Face-to-face interviews were conducted to obtain the data. We transcribed the interviews verbatim and analyzed them using reflexive thematic analysis to obtain the themes and subthemes.

Findings

Client-related challenges included difficulty in managing antiretroviral (ARV) drug reactions of clients and their financial, follow-up, and index tracking problems; facility-related challenges included inadequate and limited space for CTC services, lack of integrated HIV services, and shortage of staff, which needed collaborative efforts to overcome; and healthcare provider-related challenges included a lack of up-to-date knowledge and limited access to health information. These challenges limit the provision of quality HIV care to the clients.

Conclusion

This study highlights the important challenges that hinder the quality of HIV services at CTCs. The implementation of appropriate infrastructure to promote the privacy and confidentiality of clients is necessary as it alleviates the burden on the jobs of healthcare providers. The findings also suggest that healthcare providers improvise solutions to meet the needs of the clients in the study setting. Permanent resolution is required to overcome the challenges in CTCs. Further research on both providers and clients should be conducted to explore the challenges in CTCs in other similar settings.
Characterization of chemical reactions of silver diammine fluoride and hydroxyapatite under remineralization conditions
Authors: Kaur M. , Shahid S. , Karpukhina N. , Anderson P. , Wong F. S. L.
Published: March 2024

Introduction

Silver Diammine Fluoride (SDF) is a clinically used topical agent to arrest dental caries. However, the kinetics of its chemical interactions with hydroxyapatite (HA), the principal inorganic component of dental enamel, are not known. The aim was to characterize the step-wise chemical interactions between SDF and HA powder during the clinically important process of remineralization.

Methods

Two grams of HA powder were immersed in 10 ml acetic acid pH = 4.0 for 2 h to mimic carious demineralization. The powder was then washed and dried for 24 h and mixed with 1.5 ml SDF (Riva Star) for 1 min. The treated powder was then air-dried for 3 min, and 0.2 g was removed and stored in individual tubes each containing 10 ml remineralizing solution. Powder was taken from each tube at various times of exposure to remineralization solution (0 min, 10 min, 2 h, 4 h, 8 h, 24 h, and 10 days), and characterized using Magic Angle Spinning-Nuclear Magnetic Resonance (MAS-NMR) spectroscopy.

Results and discussion

19F MAS-NMR spectra showed that calcium fluoride (CaF2) started to form almost immediately after HA was in contact with SDF. After 24 h, the peak shifted to −104.5 ppm suggesting that fluoride substituted hydroxyapatite (FSHA) was formed with time at the expense of CaF2. The 31P MAS-NMR spectra showed a single peak at 2.7 ppm at all time points showing that the only phosphate species present was crystalline apatite. The 35Cl MAS-NMR spectra showed formation of silver chloride (AgCl) at 24 h. It was observed that after the scan, the whitish HA powder changed to black color. In conclusion, this time sequence study showed that under remineralization conditions, SDF initially reacted with HA to form CaF2 which is then transformed to FSHA over time. In the presence of chloride, AgCl is formed which is subsequently photo-reduced to black metallic silver.
Model-based monocular 6-degree-of-freedom pose tracking for asteroid
Authors: Tang Hao , Liu Chang , Su Yuzhu , Wang Qiuyin , Hu Weiduo
Published: February 2024

In this paper, we present a novel vision-based framework to track the 6-DoF pose of an asteroid in real time with the 3D contour of the asteroid as a feature. During pose tracking, at the beginning time of tracking, the tracking system is initialized by a pose retrieval method. At each subsequent time instant, given the 3D mesh model of an asteroid, with the initial pose and its covariance given by the square root cubature Kalman Filter (SCKF), the 3D mesh segments constituting the 3D asteroid contour are efficiently extracted from the 3D mesh model. Then, in the input asteroid image, we search the image points corresponding to the extracted 3D segments within the searching range defined by the initial pose and its covariance. After that, the asteroid pose is determined in real time by minimizing the angles between the back-projection lines of the searched image points and the projection planes of the corresponding 3D segments, which is much more robust to the position change of the asteroid and asteroid size. The covariance matrix of the pose is inferred from the Cartesian noise model in the first order. Eventually, the SCKF is derived from the second-order auto regression to generate the final pose estimate and give the initial pose and its covariance for the next time instant. The synthetic trials quantitatively validate the real-time performance, robustness, and accuracy of our algorithm in dark space, different imaging distances, lighting conditions, image noise, model error, and initial pose error, and meanwhile, the real trial qualitatively shows the effectiveness of our method.
Both de-growth and a-growth to achieve strong and weak sustainability: a theoretical model, empirical results, and some ethical insights
Authors: Zagonari Fabio
Published: March 2024

This study conceptually characterizes and theoretically represents the four main sustainability paradigms (strong sustainability, weak sustainability, de-growth, and a-growth) in terms of equality and inequality. It then applies these conditions to developed and less-developed countries (OECD and non-OECD countries) and empirically shows that the change in production technology (∆θ) required by a-growth is impossible and the change in consumption preferences (∆α) required by de-growth is unfeasible. Finally, it combines a-growth and de-growth in a theoretical solution for the relationship between ∆α and ∆θ that meets the conditions required by both strong and weak sustainability (parameters are the world’s population, consumption preferences, and production technologies or concerns for nature and future generations in developed and less-developed countries) and empirically demonstrates that this solution is feasible. In particular, sustainability turns out to be an ethical issue more than a technological issue, and the ethical concern for nature turns out to be more favorable than the ethical concern for future generations. Ethical assumptions and implications of the four main sustainability paradigms are highlighted and ethical assumptions and implications of the combined theoretical model are discussed. In particular, intergenerational efficiency is achieved in terms of welfare, and intergenerational equity is achieved in terms of environmental status.
How the Spread of Young Coral Can Help Save Coral Reefs
Authors: Greiner Ariel , Andrello Marco , Krkošek Martin , Fortin Marie-Josée
Published: February 2024

Coral reefs around the world are getting sick (and sometimes dying) at alarming rates due to climate change. Certain coral reefs (low-risk reefs) are predicted to be less at risk of getting sick than others. We wondered whether low-risk reefs can help save other reefs. We found that this is possible—through the movement of young coral from healthy reefs to damaged or dying reefs. We found that coral reefs worldwide are connected through the spread of young coral, forming 604 reef networks. Some networks are very large, but most are very small. If only the low-risk coral reefs survive, many of these networks will be preserved, which will help the overall survival of coral reefs into the future. However, young coral from these low-risk reefs do not reach all reefs worldwide, so it is important to find and protect reefs that are good at sending young coral to rescue the rest of the world’s reefs.
Opportunities and challenges organo-mineral fertiliser can play in enabling food security
Authors: Sakrabani Ruben
Published: March 2024

Food security is a growing challenge related to an increasing global population. The agricultural sector is key for a secure supply of food but relies up to 50% on mineral fertilisers to meet crop nutrient demands. As mineral fertilisers production is energy intensive, causing close to 2% of global greenhouse gas (GHG) emissions, this poses greater challenge to meet net zero targets. Other challenges include extreme weather patterns, GHG during fertiliser applications and diffuse pollution, declining soil health, pest, disease, and loss of soil biodiversity. As mineral fertilisers’ price increases and the state of soil health decreases, innovative solutions are needed to meet crop nutrient demands while ensuring that sufficient organic matter is conserved in the soil. One solution to achieve net zero in agriculture can be in the form of organo-mineral fertilisers (OMF). OMFs are a new concept that take organic feedstock (such as biosolids, livestock manure, crop residues, food waste) and combines them with reduced amounts of mineral fertilisers resulting in a balanced fertiliser product. This Perspective piece discusses a Strength-Weakness-Opportunities-Threats (SWOT) analysis on OMF and summarizes how OMF applications can play a role to improve food security. This is further linked with short, medium and long terms policy interventions that can be deployed to achieve a more sustainable approach by balancing between protecting the wider environment and meeting food security.
Sodium butyrate impedes the lymphoma caused by Marek’s disease virus via regulating the mitochondrial apoptosis pathway
Authors: Lin Qiaoer , Zhou Jun , Yang Fan , Zheng Congsen , Chen Meiting , Chang Chuanzhe , Cai Shikai , Wen Feng , Wang Nina , Chen Yanfeng , Qin Limei
Published: February 2024

Sodium butyrate (NaB) has garnered attention in recent years for its ability to impede the malignant progression of tumors. In order to explore the potential inhibitory effects of NaB on the replication of Marek’s disease virus (MDV) and subsequent lymphoma formation, newly hatched chickens were infected with the vvMDV Md5 strain and administered NaB prior to (prevention group) or following (treatment group) Md5 inoculation. The results revealed that NaB played a pivotal role in diminishing both the incidence and fatality rates in chickens afflicted with Md5 infection. Notably, NaB exhibited a remarkable capacity to inhibit the expression of MDV immediate early genes, i.e., ICP4 and ICP27, thus attenuating tumorigenesis in the chicken spleen. To further elucidate the mechanism of NaB on lymphoma cells, MDV bearing lymphoma cells, i.e., MSB-1 were exposed to NaB for 24 h prior to various experimental tests. The results revealed that NaB effectively hindered the proliferation, migration, and colony formation of MSB-1 cells. Furthermore, NaB demonstrated the ability to modulate the key molecules in mitochondrial apoptosis pathway. Taken together, these findings reveal that NaB can impede the lymphoma caused by MDV via regulating the mitochondrial apoptosis pathway, both in vitro and in vivo. These results suggest that the utilization of NaB warrants serious consideration as a promising approach for the prevention of MDV.
Review of Molecular Technologies for Investigating Canine Cancer
Authors: Kehl Alexandra , Aupperle-Lellbach Heike , de Brot Simone , van der Weyden Louise
Published: March 2024

Genetic molecular testing is starting to gain traction as part of standard clinical practice for dogs with cancer due to its multi-faceted benefits, such as potentially being able to provide diagnostic, prognostic and/or therapeutic information. However, the benefits and ultimate success of genomic analysis in the clinical setting are reliant on the robustness of the tools used to generate the results, which continually expand as new technologies are developed. To this end, we review the different materials from which tumour cells, DNA, RNA and the relevant proteins can be isolated and what methods are available for interrogating their molecular profile, including analysis of the genetic alterations (both somatic and germline), transcriptional changes and epigenetic modifications (including DNA methylation/acetylation and microRNAs). We also look to the future and the tools that are currently being developed, such as using artificial intelligence (AI) to identify genetic mutations from histomorphological criteria. In summary, we find that the molecular genetic characterisation of canine neoplasms has made a promising start. As we understand more of the genetics underlying these tumours and more targeted therapies become available, it will no doubt become a mainstay in the delivery of precision veterinary care to dogs with cancer.
Self-Reported Utilization of International (ACVIM Consensus) Guidelines and the Latest Clinical Trial Results on the Treatment of Dogs with Various Stages of Myxomatous Mitral Valve Degeneration: A Survey among Veterinary Practitioners
Authors: van Staveren Marie D. B. , Muis Esther , Szatmári Viktor
Published: March 2024

Background: Myxomatous mitral valve degeneration is the most common canine heart disease. Several clinical trials have investigated various treatments. The latest recommendations are published in the ACVIM consensus guidelines (2019). Our study aimed to investigate how closely veterinary practitioners apply the treatment recommendations of these guidelines and the latest clinical trials. Methods: An online survey was sent to Dutch and Belgian veterinary practices via digital channels. Results: The data from 363 fully completed surveys were analyzed. For stage B1 disease, 93% recommended, correctly, no treatment. For stage B2 disease, 67% of the respondents recommended starting pimobendan as monotherapy. For chronic treatment of stage C disease, 16 different drug combinations were mentioned, but nobody recommended surgery. Only 48% of the respondents recommended the only evidence-based drug combination: a loop diuretic with pimobendan. A concerning finding was the simultaneous prescription of two loop diuretics, by 19% of the respondents. Conclusions: Treatment recommendations showed an increasing variation with more advanced disease stages from B1 through B2 to C. This reflects the increasing disagreement among the panelists who prepared the ACVIM consensus guidelines. Practitioners of our study seem to practice more evidence-based medicine than veterinary cardiologists, as it was reported in a recent survey-based study.