bluuebunny/crossref_metadata_2025 huggingface dataset:
La polyphonie pour dire la violence dans la littérature contemporaine francophone
Authors: Ferreri Natalia Lorena
Published: June 2024

Cette contribution propose une analyse des formes de l’hétérogénéité énonciative –montrée et constitutive– (Authier-Revuz, 1984) dans deux romans francophones contemporains : Après nous les nuages  (Bruxelles, 2017) de Marc Meganck, et Belle merveille (Paris, 2017) de l’auteur haïtien James Noël,  dans le but de mettre en évidence comment à partir des énoncés polyphoniques l’on construit un discours collectif sur la violence. Ces textes racontent des événements qui ont eu lieu récemment et où la violence provoque la mort, la peur, l’horreur et le chagrin.  Dans les deux cas, le temps du récit se situe après les événements, c’est-à-dire une fois que les paysages urbains et naturels ont été modifiés par la violence. Face à ces images effrayantes, la voix narrative se démultiplie en raison d’une violence qui n’est pas individuelle, personnelle ou intime mais collective et plurielle, et qui touche une communauté tout entière. D’une part, Noël raconte une catastrophe, le tremblement de terre en Haïti en 2010, à travers une multitude de voix —des survivants et des témoins—, ainsi qu’à travers le narrateur appelé « Bernard » ; d’autre part, Meganck décrit les attentats déclenchés à Paris et à Bruxelles en 2015 et 2016, respectivement, en employant une voix anonyme qui retransmet ce qu’elle voit et entend dans les villes.  Nous postulons ici que la polyphonie devient l’instrument esthétique pertinent pour aborder un sujet actuel, urgent et collectif. Dans les deux romans, la polyphonie ne sert pas de marqueur de distance par rapport à l’énoncé, mais sert à souligner le thème, car chaque voix ajoute son point de vue pour mettre l’emphase sur la manière de raconter la violence. Ainsi l’objectif de cette étude est-il d’analyser les traces de la polyphonie dans les deux romans aux niveaux syntaxique, lexical et typographique —des marques venant interférer le fil du discours sous la forme d’une autre langue, d’un autre interlocuteur ou d’un autre registre— en vue de distinguer les identités narratives (Ricoeur, 1990) qui assument le récit dans les deux romans. Pour ce faire, nous partons du concept de dialogisme développé par Bakhtine (1989) au moyen duquel l’auteur exprime que « la plurivocalité et le plurilinguisme pénètrent dans le roman de telle sorte qu’ils sont organisés dans un système artistique » (116). Nous identifierons également les différents je du discours et les façons de les nommer ; de même que l’usage de l’italique, des guillemets et du discours indirect libre. Chemin faisant, nous mettrons en évidence comment ces voix construisent un sens collectif sur la violence, et même à quel point il faut dire la violence à plusieurs voix pour pouvoir la supporter.  C’est dans l’intention de mettre à jour les concepts de polyphonie, d’hétérogénéité énonciative et de narrateur/énonciateur que nous proposons une approche analytique dans la littérature francophone principalement contemporaine.
Arquivo, guerra e entretenimento: Pynchon como literatura documental a partir de Derrida e Kittler
Authors: Lamha Leonardo Petersen
Published: June 2024

Partindo do conceito de arquivo desenvolvido por Derrida, este artigo explora as questões midiáticas e tecnológicas, mencionadas por Derrida na sua conceptualização, em diálogo com a teoria da mídia e da literatura de Friedrich Kittler. Argumenta-se que a noção kittleriana de mídia não só continua as questões postas por Derrida, como também vai além de sua “gramatologia”, isto é, de questões filosóficas, semióticas e linguísticas e adentra no reino tecnológico, pouco explorado pelos estudos literários. Como estudo de caso, discute-se a leitura kittleriana do romance O arco-íris da gravidade, de Thomas Pynchon, buscando como o “teatro bélico” da Segunda Guerra Mundial, representada por Pynchon, esconde uma verdadeira máquina arquivística da pós-modernidade em seus aspectos midiáticos e tecnológicos.
Fratura insidiosa: a escrita do trauma em Os Marginais de João Melo
Authors: Pereira Júnior Vilarino
Published: June 2024

No conto Os Marginais, João Melo empreende o feito de mostrar que todas as saídas potenciais para a revisão da história angolana pós 1975 deverão passar, necessariamente, pelo crivo da linguagem e da subjetividade, e que a prosa de ficção permite a construção de “evidências” para as “destrutivas” experiências traumáticas, fazendo do enlace narrativo o lugar das potencialidades do enlace social. Utiliza-se, aqui, de conceitos psicanalíticos para a abordagem de uma escrita do trauma.
Memória e História em Mestre Tamoda, de Uanhenga Xitu
Authors: Mineiro Maria Aparecida , Lopes de Almeida Carlos Henrique
Published: June 2024

Este estudo propõe analisar a representação do passado, enfatizando como o elemento memória constitui-se no âmbito da ficção na obra angolana Mestre Tamoda, de Uanhenga Xitu, publicada pela primeira vez em 1974. Isso porque nessa narrativa, percebe-se um acontecimento histórico constitutivo do fazer literário. Ademais, demonstrar-se-á como essa construção literária demonstra um posicionamento ideológico sobre a reescritura dos fatos ocorridos durante a colonização angolana. Nesse sentido, o objetivo deste estudo será o de apresentar alguns artifícios da referida obra à luz da teoria da memória sobre o contexto colonizado a partir da ótica de Uanhenga Xitu, que por sua vez, apropria-se de elementos memorialísticos para compor sua narrativa, bem como fatos da história para a ficcionalização de suas lembranças. Portanto, a análise a ser realizada, propicia a compreensão de como as memórias coletivas, individuais e históricas delineiam o cenário proposto no conto Mestre Tamoda, de Uanhenga Xitu, a ponto de indicar a resistência de sua “sanzala” diante da imposição da cultura portuguesa. Por este caminho, analisaremos o romance de Uanhenga Xitu, que reflete sobre o próprio processo de elaboração artística e, ao mesmo tempo, utiliza a história e a memória como fios condutores da organização da obra. A discussão proposta fundamentar-se-á em Peter Burke (1992); Hayden White (1994); Seligmann-Silva (2003); Paolo Rossi (2010); Aleida Assmann (2011) e Halbwachs (2013).
DAMPAK PASTORAL SEKOLAH TERHADAP PEMBENTUKAN KARAKTER PESERTA DIDIK AGAMA KATOLIK SMP KATOLIK STA. MARIA ASSUMPTA KOTA BARU KUPANG
Authors: Ta’ek Emilia Dolorosa , Je’eMaly Emanuel Inocentius , Masan Valentinus Kopong
Published: June 2024

The aim of this research is to determine the influence of school pastoralism on the character formation of Catholic religious students at St. Catholic Middle School. Maria Assumpta New City Kupang. This study uses a qualitative method. The sample in this study was 30 people consisting of 1 school teacher, 24 teachers and 5 people.  The data collection technique used in the research was carried out using interviews and documentation studies, while the data analysis technique in this qualitative research was by using the power collection, data reduction, data display and data ferifying techniques. The results of the research show that school pastoral care has a huge impact in shaping the character of Catholic students, this cannot be separated from the support and assistance of parents. This is shown through the results of research using interview methods with school principals, teachers and parents.
Developing and Validating an EFL Teacher Professional Identity Inventory: A Mixed Methods Study
Authors:   Kharazmi University, Hashemi Mohammad R. , Karimi Mohammad Nabi , Mofidi Mahdieh
Published: June 2024

In line with the exponential growth in English as a foreign language (EFL) teacher professional identity research, the present study was an attempt to develop and validate a questionnaire on EFL teacher professional identity. Following Pennington (2015), we defined EFL teacher professional identity as 'a unique blend of individual teacher characteristics within the disciplinary knowledge, standards, and practices of the field' (p. 78), which is further comprised of a set of core and peripheral elements (Beijaard et al., 2004). In order to operationalize this construct, a sequential mixed methods design was utilized. Hence, a total number of 24 participants and 750 EFL teachers took part in the qualitative and quantitative phases respectively. Analysis of the qualitative data revealed the features characterizing EFL teacher professional identity. On the basis of the qualitative results, the first version of the inventory was developed through exploratory factor analysis and was validated using structural equation modeling. In general, the results of the study paint a multi-dimensional picture of EFL teacher professional identity. This picture depicts the core features of EFL teacher professional identity and a number of peripheral elements which change and harmonize in response to the individual, contextual, and socio-cultural factors. (Beijaard et al., 2004).
FINANCIAL LITERACY TRAINING TO INCREASE SELF-CAPACITY STUDENTS AT IT IZZATUNA HIGH SCHOOL
Authors: Lidia Desiana  , Muhamad Rahman Bayumi  , Nur Hesthria  , Fernando Africano
Published: June 2024

Islamic banking is currently growing rapidly and this can be seen from the increasingly complete Islamic bank products. The presence of Islamic bank products can certainly be an option in order to prioritize Islamic principles. Basically, Islamic banks carry out the same three functions as conventional banks, it's just that Islamic bank products have very different principles from conventional banks. Islamic bank products generally apply a profit-sharing system that is mutually beneficial for customers and banks. In this article, we will discuss further about the products and services contained in Islamic banks.
Mengembangkan Budaya Literasi untuk Masyarakat Desa Lengkong Kecamatan Bojongsoang Kabupaten Bandung
Authors: Subiyono Subiyono , Trie Ramadhany Gunawan Marina , Darul Harbi Dani , Nurbaeti Nurbaeti , Fadlan Sandy , Pardiansyah Dedi , Sutiawati Sutiawati , Rudiansyah Ahmad
Published: June 2024

Segala upaya untuk memampukan dan membiasakan membaca bagi warga merupakan salah satu investasi untuk pembangunan berkelanjutan. Agar kegiatan membaca menjadi tradisi dan budaya bangsa, maka perlu dilakukan pembiasaan sejak dini. Tujuan pengabdian yakni mengembangkan budaya literasi untuk masyarakat Desa Lengkong Kecamatan Bojongsoang Kabupaten Bandung. Metode pengabdian yang dilakukan yakni tahap persiapan, pelaksanaan dan evaluasi. Berdasarkan hasil pelaksanaan kegiatan terlihat bahwa Segala upaya untuk memampukan dan membiasakan membaca bagi warga merupakan salah satu investasi untuk pembangunan berkelanjutan. Agar kegiatan membaca menjadi tradisi dan budaya bangsa, maka perlu dilakukan pembiasaan sejak dini. Dalam turut serta mensukseskan program literasi yang dicanangkan pemerintah, maka kami Kelompok 2 PKM STAI Sabili menyelenggarakan program Habituasi Literasi.
Validez y confiabilidad de la Escala de Estrés Percibido: la adaptación a estudiantes universitarios después de la pandemia
Authors: Andraca Sánchez Ciro , Muñoz García Alejandra Hitahii , González González Justiniano , Mendoza Mojica Manuel , Bueno Domínguez Paulino
Published: June 2024

El objetivo del presente estudio fue evaluar la validez y confiabilidad de la escala de estrés percibido (PSS) en estudiantes de la Universidad Autónoma de Guerrero, México. El estudio transversal incluyó a 116 mujeres y 24 hombres, de las licenciaturas de Enfermería y Ciencias de la Educación, a quienes se les aplicó un cuestionario que incluyó la realización de estadísticos descriptivos e inferenciales, con respecto a las variables socioeconómicas exploradas, además incluyó el análisis del estrés percibido entre hombres y mujeres, con valores significativos (p < 0,05). La segunda parte, incluyó el análisis del estrés percibido, a partir del diseño original de la Perceived Stress Scale (PSS-14) implementada en población estadounidense, así como versiones  adaptadas a otros países. La validez del constructo incluyó el fundamento teórico sobre el sentido positivo y negativo de los ítems:  0 = nunca, 1= casi nunca, 2 = de vez en cuando, 3 = a menudo y 4 = muy a menudo para los negativos, y con sentido inverso para los positivos. La confiabilidad (alfa de Cronbach) fue de 0,767 considerado como un valor adecuado. En conclusión, la adaptación del cuestionario resultó fiable y válido para el análisis del estrés percibido en poblaciones culturalmente similares.
Plantas medicinales de la comunidad de Tlatlauquitepec, Atlixtac,
Authors: DE JESÚS SÁNCHEZ CONRADO ALDEHIR
Published: June 2024

En este estudio se buscó documentar las plantas medicinales de la comunidad, rescatar y su revalorizar el conocimiento sobre la herbolaria y comenzar con los procesos de producción o de recolección controlada de las especies encontradas en la investigación, ya que, en la comunidad de Tlatlauquitepec, municipio de Atlixtac, Guerrero, se encuentra nulo registro sobe las plantas medicinales, esto es de vital importancia para preservar el conocimiento tradicional que se ha venido perdiendo debido a diversos fenómenos tales como la destrucción de bosques y la disminución de diversidad biológica, así como de la pérdida cultural.  Se encontraron 16 especies distribuidas de la siguiente manera: 5 especies de la Fabaceae, 4 de la familia Asteraceae, una especie de las siguientes familias Lamiaceae, Myrtaceae, Brassicaceae, Acanthaceae, Vitaceae, Lamiaceae y Malvaceae.
MANAJEMEN LAYANAN PERPUSTAKAAN DALAM MENINGKATKAN MINAT BACA SISWI di MTs DARUNNAJAH 2 CIPINING BOGOR
Authors: Adisty Qurotul Aini  , Abdul Saipon  , M. Yogi Saputra
Published: June 2024

Penelitian ini dilatar belakangi dengan kurangnya minat dan keinginan siswi akan budaya membaca. Siswi juga kurang memahami tentang perpustakaan. Tujuan dari penelitian ini adalah untuk mengetahui manajemen perpustakaan yang ada di MTs Darunnajah 2 Cipining Bogor untuk meningkatkan minat baca peserta didik. Jenis penelitian yang digunakan adalah penelitian deskriptif kualitatif. Subjek dalam penelitian ini adalah Kepala Sekolah perpustakaan dan 6 orang siswi dari kelas IX Hasil penelitian menunjukkan bahwa implementasi manajemen perpustakaan di MTs Darunnajah 2 Cipining Bogor sudah berjalan sesuai dengan Sistem Manajemen yang baik, tetapi perlu dilakukan evaluasi untuk meminimalisir kekurangan-kekurangan yang terjadi di lapangan. dari aspek perencanaan, perpustakaan MTs Darunnajah 2 Cipining Bogor telah merencanakan kegiatan dalam penyelenggaraan perpustakaan yang meliputi pengadaan bahan koleksi, anggaran dan sumber daya manusia. Dari aspek pengorganisasian, perpustakaan MTs Darunnajah 2 Cipining Bogor sudah membuat struktur organisasi dengan baik, sehingga dapat memperlancar kinerja perpustakaan sesuai dengan pekerjaannya masing-masing. Dari aspek penggerakkan dalam manajemen perpustakaan meliputi pelayanan dan penyediaan sarana prasarana. Dari aspek pengawasan, pengawasan yang dilakukan berupa pengawasan rutin setelah selesai melaksanakan program kerja.
The Relationship between Emotional Intelligence and Academic Achievement in a Lebanese Sample
Authors:   , Hazimeh Fayza Fawzi
Published: June 2024

The main purpose of this study was to examine the relation between emotional intelligence and academic achievement in seventh and tenth grade classrooms (N= 149:81 males and 68 females). It was a correlational study that utilized the Six Seconds Emotional Intelligence Assessment for Youth and the Students' academic records (2009-2010). The results indicated no correlation between general academic averages and emotional intelligence scores (EQ). There was a positive correlation between emotional intelligence (EI) subscales and academic subjects but no correlation between age and emotional intelligence. The results also indicated that there is no significant difference between the performance of females and males in emotional intelligence. This work indicates that nonintellectual skills could be contributing factors to students' academic and maybe life success.
The Impact of Mothers' Attachment Style on their Children's Psychopathology and its Interconnectedness with Perceived Security in Parents
Authors:   , Chedid Carla
Published: June 2024

Attachment style of humans could explain a wide variety of their personality traits, especially behavioral maladjustments. The purpose of the study was to determine the effect of attachment style of mothers on the exhibition of externalizing and internalizing behaviors in their children. Further investigations were done to verify how children behave when they perceive their parents as an insecure attachment figure and if this perceived security will be associated to the mother attachment style. The sample was composed of 139 mothers and their adolescents' children. Mothers completed a revised version of the Attachment Style Questionnaire and the Children behavioral Checklist. Adolescents completed the Inventory of Parent and Peer attachment. Findings supported that children of fearful and preoccupied mothers have exhibited internalizing and externalizing behaviors. In addition, perceived security in parents affects adolescents' behaviors.
Level of Online Media Literacy of Collegiate Students: Basis for a Proposed Curriculum Enhancement Program
Authors: Riparip Eduard , Caballes Dennis
Published: June 2024

As early as the 2000s, the growing demand to integrate digital skills as competencies into the curriculum has been widely advocated by media scholars (Hobbs, 2009; Hobbs & Jensen, 2013; Bautista, 2021). Among these digital skills is online media literacy, which, according to Hallaq (2016), impacts students’ acquisition of knowledge. Media literacy is defined as “the ability to access, analyze, evaluate, and create messages across a variety of contexts" (Livingstone, 2004). The five components of online media literacy formulated by Hallaq (2016) used in this study are ethical awareness, media access, media awareness, media evaluation, and media production. This study aimed to assess the level of online media literacy of Centro Escolar University-Makati students based on Hallaq's Digital Online Media Literacy Assessment (DOMLA). Using convenience sampling, 374 college students participated in the survey. Data collected were analyzed using statistical tools such as frequency, weighted mean, and the Kruskal-Wallis Test. The findings revealed that the students are slightly online media literate. Specifically, they are literate in ethical awareness and media awareness. The results also indicated no significant difference in the level of online media literacy across different ages and year levels, suggesting that media literacy is consistently moderate across different demographics. The study recommends a contextualized and interactive digital media literacy curriculum in college, which could either be separate and independent or integrated into various general education and professional subjects. Additionally, universities are encouraged to adopt the Vowel Approach in Online Media Literacy (awareness, education, integration, optimization, and utilization) formulated by the researchers.
UNIFIDE EMPLOYEE DATA TEAM TRCKER
Authors: Khorgade Laxmi
Published: June 2024

Abstract : A Team Track: “Unified Employee Data” provides a thorough overview of important metrics, tasks, and insights pertinent to each individual’s function inside a business, acting as a centralized center for them. With the use of this digital technology, staff members can easily access critical information, improving productivity, efficiency, and transparency all around. Fundamentally, an employee dashboard compiles information from multiple sources—such as project management software, HR systems, and performance metrics—and presents it in an intuitive manner. Employees can monitor their progress, evaluate critical updates, and remain up to date on company-wide efforts thanks to this consolidation. With its many features and functionalities, the employment dashboard acts as a focal point for effective job matching and recruitment. The dashboard uses data analytics and state-of-the-art technology to give users real-time insights into market trends, demand signals, and talent assessments. This allows users to make well-informed decisions regarding their hiring strategies and career routes. The dashboard gives staff members the ability to efficiently manage their obligations by giving them access to training materials and project timetable tracking.Additionally, the employee dashboard encourages teamwork and communication. Through the provision of real-time project progress updates Yes, the following are some important details you should provide in an abstract regarding an employee dashboard: Overview: Give a succinct explanation of the purpose of an employee dashboard in contemporary work environments. Mention how it provides a consolidated platform where staff members may access a range of tools, services, and data pertaining to their jobs and companies. Features: Emphasize the key elements that are commonly present in an employee dashboard, such as customized employee profiles, task management tools, collaboration features, access to corporate announcements, HR papers, and payroll data. Benefits: Talk about how having an employee dashboard can lead to better decision-making through real-time data analytics, increased productivity through streamlined resource access, improved communication and transparency within the company, and enhanced employee engagement. Index Terms – Chatgpt, AI tool, Java script
A Thorough Analysis of the Address Corrector: Improving Validity of Data and Reliability
Authors: Choudhary Vikas
Published: June 2024

Abstract : This paper explores the creation of the Shatam Address Corrector, a complex system based on contemporary technologies like Java, Jetty, AWT, and Docker for robust backend development, HTML, CSS, and JavaScript for smooth frontend implementation, and Lucene for effective data indexing and searching. Fundamentally, the system is built to parse more than 10,000 addresses in ten seconds or less, demonstrating its fast processing power. The main goal of this project is to provide an intuitive user interface that will enable users to accurately and systematically arrange their address data in an easy-to-use manner. This system seeks to optimize address rectification by utilizing state-of-the-art technology design concepts. It focuses on improving accuracy and efficiency for addresses in the United States and Canada. This paper explains the broad objectives of the project, goes into great detail on the complex technological architecture of the Shatam Address Corrector, and emphasizes how crucial this system is to transforming the address correction industry. This research clarifies the critical role that the Shatam Address Corrector plays in developing address correction techniques for the present day by thoroughly examining its goals, design, and relevance.
Självreglerat lärande: Kan självreglerat lärande bidra till ökat ansvarstagande hos eleven?
Authors: Hoff Jenny
Published: June 2024

Elevernas förmåga till ansvarstagande upplever jag har försvagats, och som yrkeslärare har denna problematik fastnat i mina tankar. När jag fick möjlighet att skriva ett utvecklingsarbete på yrkeslärarprogrammet ville jag utforska området, vilket landade i att jag utformade ett arbetsområde där jag undersökte om principer för självreglerat lärande kan bidra till ett ökat ansvarstagande hos eleverna. Arbetet förankrades i litteratur som lyfter fram olika arbetssätt inom självreglerat lärande, med fokus på hur elever utvecklar förmågor som exempelvis självbedömning och självreflektion, som kan bidra till ökat ansvarstagande. Men vad innebär självreglerat lärande, och på vilket sätt skulle det kunna bidra till ett ökat ansvarstagande i studierna? Deltagare var elever på gymnasiet i årskurs tre på Hantverksprogrammet med yrkesutgång Hår och makeupstylist. De genomförde ett case innefattande styling med hår och makeup inför fotografering i både svartvitt och färg. Det handlade om arbetsuppgifter i flera steg som pågick under några veckor. Reflektionsloggar användes för att bearbeta förmågor inom självreglerat lärande. För att utröna om elevernas förståelse kan ha ökat ansvarstagandet tittade jag efter hög eller låg kvalitetsnivå inom självbedömning och självkontroll utifrån de reflektioner eleverna skrivit i sina loggböcker.
Redo för digitalisering?
Authors: Begovic Edina , Gustafsson Jennifer , Enochsson Ann-Britt
Published: June 2024

Denna studie syftar till att bidra med kunskap om i vilken utsträckning blivande förskollärare känner sig redo att arbeta med förskolans digitaliseringsarbete efter avslutad förskollärarutbildning. Deltagare i studien är förskollärarstudenter i slutet av förskollärarutbildningen. I studien undersöks i vilken utsträckning förskollärarstudenterna har haft möjlighet att ta del av någon form av undervisning om hur de skulle kunna arbeta med förskolans digitalisering under sin utbildning. En digital enkät med både förkodade och öppna frågor användes. Resultatet visar att de blivande förskollärarna själva upplever att de har goda kunskaper och kompetenser om för-skolans digitaliseringsuppdrag, och att de känner sig redo att arbeta med detta efter avklarad förskollärarutbildning. Samtidigt uppger majoriteten av respondenterna att de inte vet vad be-greppet adekvat digital kompetens handlar om, trots att det är vad de enligt läroplanen förväntas arbeta med. Artikeln diskuterar hur de blivande förskollärarna ska kunna lyckas med sitt uppdrag utifrån dessa förutsättningar.
The αC-β4 loop controls the allosteric cooperativity between nucleotide and substrate in the catalytic subunit of protein kinase A
Authors: Olivieri Cristina , Wang Yingjie , Walker Caitlin , Subrahmanian Manu Veliparambil , Ha Kim N , Bernlohr David , Gao Jiali , Camilloni Carlo , Vendruscolo Michele , Taylor Susan S , Veglia Gianluigi
Published: June 2024

Allosteric cooperativity between ATP and substrates is a prominent characteristic of the cAMP-dependent catalytic subunit of protein kinase A (PKA-C). This long-range synergistic action is involved in substrate recognition and fidelity, and it may also regulate PKA’s association with regulatory subunits and other binding partners. To date, a complete understanding of this intramolecular mechanism is still lacking. Here, we integrated NMR(Nuclear Magnetic Resonance)-restrained molecular dynamics simulations and a Markov State Model to characterize the free energy landscape and conformational transitions of PKA-C. We found that the apoenzyme populates a broad free energy basin featuring a conformational ensemble of the active state of PKA-C (ground state) and other basins with lower populations (excited states). The first excited state corresponds to a previously characterized inactive state of PKA-C with the αC helix swinging outward. The second excited state displays a disrupted hydrophobic packing around the regulatory (R) spine, with a flipped configuration of the F100 and F102 residues at the αC-β4 loop. We validated the second excited state by analyzing the F100A mutant of PKA-C, assessing its structural response to ATP and substrate binding. While PKA-CF100A preserves its catalytic efficiency with Kemptide, this mutation rearranges the αC-β4 loop conformation, interrupting the coupling of the two lobes and abolishing the allosteric binding cooperativity. The highly conserved αC-β4 loop emerges as a pivotal element to control the synergistic binding of nucleotide and substrate, explaining how mutations or insertions near or within this motif affect the function and drug sensitivity in homologous kinases.
Prdm1 Positively Regulates Liver Group 1 ILCs Cancer Immune Surveillance and Preserves Functional Heterogeneity
Authors: He Jitian , Gao Le , Wang Peiying , Chan Wing Keung , Zheng Yiran , Zhang Yumo , Wang Jiming , Chen Huaiyong , Yang Zhouxin , Wang Youwei
Published: June 2024

Group 1 innate lymphoid cells (ILCs) comprise conventional natural killer (cNK) cells and type 1 innate lymphoid cells (ILC1s). The main functions of liver cNK cells and ILC1s not only include directly killing target cells but also regulating local immune microenvironment of the liver through the secretion of cytokines. Uncovering the intricate mechanisms by which transcriptional factors regulate and influence the functions of liver cNK cells and ILC1s, particularly within the context of liver tumors, presents a significant opportunity to amplify the effectiveness of immunotherapies against liver malignancies. Using Ncr1-drived conditional knockout mouse model, our study reveals the regulatory role of Prdm1 in shaping the composition and maturation of cNK cells. Although Prdm1 did not affect the killing function of cNK cells in an in vivo cytotoxicity model, a significant increase in cancer metastasis was observed in Prdm1 knockout mice. Interferon- gamma (IFN-γ), granzyme B, and perforin secretion decreased significantly in Prdm1 deficient cNK cells and liver ILC1s. scRNA sequencing data also provided evidences that Prdm1 maintains functional subsets of cNK cells and liver ILC1s and facilitates communications between cNK cells, liver ILC1s and macrophages. The present study unveiled a novel regulatory mechanism of Prdm1 in cNK cells and liver ILC1s, showing promising potential for developing innovative immune therapy strategies against liver cancer.
Mapping the molecular motions of 5-HT3 serotonin-gated channel by voltage-clamp fluorometry
Authors: Peverini Laurie , Shi Sophie , Medjebeur Karima , Corringer Pierre-Jean
Published: June 2024

The serotonin-gated ion channel (5-HT3R) mediates excitatory neuronal communication in the gut and the brain. It is the target for setrons, a class of competitive antagonists widely used as antiemetics, and is involved in several neurological diseases. Cryo-electron microscopy (cryo-EM) of the 5-HT3R in complex with serotonin or setrons revealed that the protein has access to a wide conformational landscape. However, assigning known high-resolution structures to actual states contributing to the physiological response remains a challenge. In the present study, we used voltage-clamp fluorometry (VCF) to measure simultaneously, for 5-HT3R expressed at a cell membrane, conformational changes by fluorescence and channel opening by electrophysiology. Four positions identified by mutational screening report motions around and outside the serotonin-binding site through incorporation of cysteine-tethered rhodamine dyes with or without a nearby quenching tryptophan. VCF recordings show that the 5-HT3R has access to four families of conformations endowed with distinct fluorescence signatures: ‘resting-like’ without ligand, ‘inhibited-like’ with setrons, ‘pre-active-like’ with partial agonists, and ‘active-like’ (open channel) with partial and strong agonists. Data are remarkably consistent with cryo-EM structures, the fluorescence partners matching respectively apo, setron-bound, 5-HT bound-closed, and 5-HT-bound-open conformations. Data show that strong agonists promote a concerted motion of all fluorescently labeled sensors during activation, while partial agonists, especially when loss-of-function mutations are engineered, stabilize both active and pre-active conformations. In conclusion, VCF, though the monitoring of electrophysiologically silent conformational changes, illuminates allosteric mechanisms contributing to signal transduction and their differential regulation by important classes of physiological and clinical effectors.
Multi level perspectives in stock price forecasting: ICE2DE-MDL
Authors: Akşehir Zinnet Duygu , Kılıç Erdal
Published: June 2024

This study proposes a novel hybrid model, called ICE2DE-MDL, integrating secondary decomposition, entropy, machine and deep learning methods to predict a stock closing price. In this context, first of all, the noise contained in the financial time series was eliminated. A denoising method, which utilizes entropy and the two-level ICEEMDAN methodology, is suggested to achieve this. Subsequently, we applied many deep learning and machine learning methods, including long-short term memory (LSTM), LSTM-BN, gated recurrent unit (GRU), and SVR, to the IMFs obtained from the decomposition, classifying them as noiseless. Afterward, the best training method was determined for each IMF. Finally, the proposed model’s forecast was obtained by hierarchically combining the prediction results of each IMF. The ICE2DE-MDL model was applied to eight stock market indices and three stock data sets, and the next day’s closing price of these stock items was predicted. The results indicate that RMSE values ranged from 0.031 to 0.244, MAE values ranged from 0.026 to 0.144, MAPE values ranged from 0.128 to 0.594, and R-squared values ranged from 0.905 to 0.998 for stock indices and stock forecasts. Furthermore, comparisons were made with various hybrid models proposed within the scope of stock forecasting to evaluate the performance of the ICE2DE-MDL model. Upon comparison, The ICE2DE-MDL model demonstrated superior performance relative to existing models in the literature for both forecasting stock market indices and individual stocks. Additionally, to our knowledge, this study is the first to effectively eliminate noise in stock item data using the concepts of entropy and ICEEMDAN. It is also the second study to apply ICEEMDAN to a financial time series prediction problem.
The inorganic pyrophosphatases of microorganisms: a structural and functional review
Authors: García-Contreras Rodolfo , de la Mora Javier , Mora-Montes Héctor Manuel , Martínez-Álvarez José A. , Vicente-Gómez Marcos , Padilla-Vaca Felipe , Vargas-Maya Naurú Idalia , Franco Bernardo
Published: June 2024

Pyrophosphatases (PPases) are enzymes that catalyze the hydrolysis of pyrophosphate (PPi), a byproduct of the synthesis and degradation of diverse biomolecules. The accumulation of PPi in the cell can result in cell death. Although the substrate is the same, there are variations in the catalysis and features of these enzymes. Two enzyme forms have been identified in bacteria: cytoplasmic or soluble pyrophosphatases and membrane-bound pyrophosphatases, which play major roles in cell bioenergetics. In eukaryotic cells, cytoplasmic enzymes are the predominant form of PPases (c-PPases), while membrane enzymes (m-PPases) are found only in protists and plants. The study of bacterial cytoplasmic and membrane-bound pyrophosphatases has slowed in recent years. These enzymes are central to cell metabolism and physiology since phospholipid and nucleic acid synthesis release important amounts of PPi that must be removed to allow biosynthesis to continue. In this review, two aims were pursued: first, to provide insight into the structural features of PPases known to date and that are well characterized, and to provide examples of enzymes with novel features. Second, the scientific community should continue studying these enzymes because they have many biotechnological applications. Additionally, in this review, we provide evidence that there are m-PPases present in fungi; to date, no examples have been characterized. Therefore, the diversity of PPase enzymes is still a fruitful field of research. Additionally, we focused on the roles of H+/Na+ pumps and m-PPases in cell bioenergetics. Finally, we provide some examples of the applications of these enzymes in molecular biology and biotechnology, especially in plants. This review is valuable for professionals in the biochemistry field of protein structure–function relationships and experts in other fields, such as chemistry, nanotechnology, and plant sciences.
Associations between fundamental movement skills and accelerometer-measured physical activity in Chinese children: the mediating role of cardiorespiratory fitness
Authors: Zhang Dingyi , Chen Sitong , Xin Fei , Drenowatz Clemens , Hu Xiaoqing , Tang Yan
Published: June 2024

Background and purpose

The associations of fundamental motor skills (FMS), health-related physical fitness (e.g., cardiorespiratory fitness, CRF), and moderate-vigorous physical activity (MVPA) have been demonstrated in Western children, but these associations have not yet been validated in a sample of Chinese children. The aims of this study, therefore, were to examine the association between FMS subdomains and MVPA in a sample of Chinese children and to evaluate whether this association is mediated by CRF.

Methods

A cross-sectional study consisting of 311 children aged 8–12 years (49.2% girls; mean age = 9.9 years) from Shanghai was conducted. FMS, CRF and MVPA were assessed using the Test of Gross Motor Development-3rd Edition, Progressive Aerobic Cardiovascular Endurance Run and ActiGraph GT3X accelerometers. Preacher & Hayes’s bootstrap method was used to test the mediating effects of CRF on the association between FMS and MVPA.

Results

CRF fully mediated the association between total FMS and MVPA in girls (indirect effects, b = 0.21, 95% CI [0.07–0.37]), while the mediation was only partial in boys (indirect effects, b = 0.12, 95% CI [0.01–0.26]). CRF fully mediated the association between locomotor skills and MVPA in girls (indirect effects, b = 0.27, 95% CI [0.09– 0.51]), whereas CRF partially mediated the association between object control skills and MVPA in boys (indirect effects, b = 0.15, 95% CI [0.18–0.35]).

Conclusion

In order to better design and implement sex-specific interventions aiming to increase MVPA, it is essential to consider FMS subdomains and CRF alongside the sex differences in the association between them.
Genomic organization and expression profiles of nitrogen assimilation genes in Glycine max
Authors: Elsanosi Hind Abdelmonim , Zhu Tiantian , Zhou Guisheng , Song Li
Published: June 2024

Background

Glutamine synthetase (GS), glutamate synthase (GOGAT), and nitrate reductase (NR) are key enzymes involved in nitrogen assimilation and metabolism in plants. However, the systematic analysis of these gene families lacked reports in soybean (Glycine max (L.) Merr.), one of the most important crops worldwide.

Methods

In this study, we performed genome-wide identification and characterization of GS, GOGAT, and NR genes in soybean under abiotic and nitrogen stress conditions.

Results

We identified a total of 10 GS genes, six GOGAT genes, and four NR genes in the soybean genome. Phylogenetic analysis revealed the presence of multiple isoforms for each gene family, indicating their functional diversification. The distribution of these genes on soybean chromosomes was uneven, with segmental duplication events contributing to their expansion. Within the nitrogen assimilation genes (NAGs) group, there was uniformity in the exon-intron structure and the presence of conserved motifs in NAGs. Furthermore, analysis of cis-elements in NAG promoters indicated complex regulation of their expression. RT-qPCR analysis of seven soybean NAGs under various abiotic stresses, including nitrogen deficiency, drought-nitrogen, and salinity, revealed distinct regulatory patterns. Most NAGs exhibited up-regulation under nitrogen stress, while diverse expression patterns were observed under salt and drought-nitrogen stress, indicating their crucial role in nitrogen assimilation and abiotic stress tolerance. These findings offer valuable insights into the genomic organization and expression profiles of GS, GOGAT, and NR genes in soybean under nitrogen and abiotic stress conditions. The results have potential applications in the development of stress-resistant soybean varieties through genetic engineering and breeding.
Differentiated Instruction on Academic Performance in Filipino Subject
Authors: Pinaranda Sharon M. , Sario Myrna B.
Published: June 2024

Aims: The purpose of this study is to investigate the effects of differentiated instructions used by the teachers on the students` academic performance in both the control and experimental groups.
Study Design: quasi-experimental method.
Place and Duration of Study: The study was conducted among 60 students, composed of a control and an experimental group of Grade 9 students from Union National High School, Monkayo East District, Division of Davao de Oro, in the year 2023–2024.
Methodology: The study used purposive sampling to identify its respondents. This study used a researcher-made questionnaire to collect the necessary data in the pretest and posttest. This research used the mean, standard deviation, and t-test to analyze the raw data obtained from the respondents. They use a t-test to determine if the experimental group's students' scores significantly differed from the control group's before and after the intervention.
Results: The main findings revealed that both the control and experimental groups showed improvement in their posttest scores compared to their pretest scores. However, the high value of the t-test indicates that there is sufficient evidence to contradict the null hypothesis. Furthermore, the findings indicate that using differentiated instruction can make learning more rewarding and easier.
Conclusion: The use of differentiated learning instruction as a teaching strategy can have a significant and positive effect on the academic success and attitude of Filipino subject. We recommend strengthening the use of different strategies in classes. Additionally, we should provide teachers with scaffolding to effectively implement the strategy in their classes.
Groundwater Quality Assessment with Respect to Heavy Metal Content by using Heavy Metal Pollution Index (HPI) of Moradabad District, Uttar Pradesh, India
Authors: Husain Saddam , Ali Gufran
Published: June 2024

Heavy metal pollution index (HPI), is a rating method to assess the water quality and to categorize the groundwater pollution with respect to heavy metals content. The aims of the present study are to look over the current status of heavy metals pollution in and around Moradabad city. Heavy metal pollution in water has gained universal consciousness due to its tenacity, accumulation in the food chain and negative effects on ecological as well as human health. Its variation in concentration can cause deterioration of water. The study focuses on examining the content of heavy metal (Zn, Fe, Cd, Mn, Pb, Ni, Cu, and Cr) in the 30 water samples from the surrounding areas of Moradabad city. The concentrations of Cd, Mn, Fe, Cu, Pb, Zn, Ni, and Cr in water samples were recorded in the pre- and post-monsoon season of 2017. The examined samples reveal that the contamination level of heavy metal is in the following sequence Ni>Fe>Pb>Cd>Cr>Cu>Zn>Mn in premonsoon 2017, whereas in postmonsoon season, heavy metals values are shown as Fe>Pb>Ni>Mn>Cr> Cu>Zn>Cd. The HPI average value in pre-monsoon 2017 is 13.07, which recommended that the groundwater quality is good. While in post-monsoon season HPI average values has been 159.26 suggesting that the water quality is poor to inferior. The correlation matrix has been evaluated and shows a positive correlation with the elements. The immense production of industrial solid waste in Moradabad city and its improper disposal in the form of heap piled outside the city area generates leachate. Heavy metal leaching from these disposal points may contaminate the groundwater as well as surface water resources. The study shows that heavy metal concentration has a noticeable rise in water due to different anthropogenic and various natural sources of contamination.
Effect of Nutrient Management on Physico-chemical Properties of Soil in Indian Mustard
Authors: Singh Anchal , Singh Narendra , Kalhapure Aniket H. , Singh S.B, , Gupta Arbind Kumar , Gupta Dinesh , Gupta Shweta , Gupta Devrani
Published: June 2024

A field experiment was conducted at the IFS Unit Instructional Farm of Banda University of Agriculture & Technology, Banda (UP) during the winter (rabi) seasons of 2022-23 and 2023-24. The study aimed to examine the impact of nutrient management on the soil properties of Indian mustard (Brassica juncea L.) grown in clay loam soil. Mustard, an essential oilseed crop in India, encounters issues like low productivity and diminished soil fertility caused by excessive use of chemical fertilizers. The paper highlights the importance of integrating chemical and organic sources, such as vermicompost. Vermicompost enriched with biofertilizers, especially Azotobacter, PSB, PMB, NPK Consortia, and ZSB demonstrates significant potential in improving the soil conditions for mustard crops. The experiment utilized a split plot design (SPD) with four main factors in the main plot and four levels in the sub-plot, resulting in sixteen treatment combinations, each applied three times. The soil at the experimental site was characterized by a clay loam texture and low levels of organic carbon, available nitrogen, phosphorus, zinc and sulfur, with moderate levels of available potash. The main plot treatments consisted of different fertility levels: 50% RNPKS, 75% RNPKS, 100% RNPKS and 125% RNPKS. Each main plot was further divided into four sub-plots, each with a combination of micronutrients and enriched vermicompost. The results indicated that the treatment of either 125% or 100% RNPKS and 2.5 kg Zn + 0.5 kg B + 500 kg enriched vermicompost ha-1 was superior in improving the soil properties for Indian mustard.
Identification of Drought Tolerant Fenugreek Mutants at Seedling Stage Through Multi-Trait Genotype-Ideotype Distance Index (MGIDI)
Authors: J. Jyothsna , Pandey S.K. , Nair Reena , Mehta A.K.
Published: June 2024

The top-performing mutants from the M2 generation, induced by a combination of γ radiation and EMS treatment, underwent screening for drought tolerance at the seedling stage in the M3 generation under controlled laboratory conditions. This investigation aimed to assess their potential for drought tolerance. The top 15 high-yielding mutants were assessed under both normal (control) and induced drought conditions (10% PEG solution). Utilizing the MGIDI method, the evaluation provided valuable insights into selection traits and identified mutants with potential drought tolerance. Under normal conditions, mutants M3-181 and M3-149 exhibited superior performance in seedling growth traits, with M3-149 being the top performer under induced drought conditions, followed by M3-181. Their proximity to the red-cutline suggests promising potential that warrants further investigation. Furthermore, these findings indicate that mutant lines displayed superior multi-trait performance compared to the parent cultivar. Specifically, mutants M3-181, M3-149, and M3-29 ranked higher than the parent cultivar under normal conditions, while mutants M3-149, M3-181, and M3-168 surpassed the parent cultivar RMt-1 under induced drought conditions. This underscores the effectiveness of mutation breeding, particularly through the induction of mutations using γ radiation and EMS, as an efficient strategy not only for generating genetic diversity but also for producing variants superior to the parent cultivar. To the best of our knowledge, this is first report made on mutant selection in fenugreek for drought tolerance at seedling stage, using the MGIDI approach.