bluuebunny/crossref_metadata_2025 huggingface dataset:
From Acute Carditis, Rheumatic Carditis, and Morphologic Cardiac Reactions to Allergic Angina, Allergic Myocardial Infarction and Kounis Syndrome: A Multidisciplinary and Multisystem Disease
Authors: Kounis Nicholas G. , Stefanidis Alexandros , Hung Ming-Yow , Özkan Uğur Özkan , de Gregorio Cesare , Ceasovschih Alexandr , Mplani Virginia , Gogos Christos , Assimakopoulos Stelios F. , Chatzigrigoriadis Christodoulos , Plotas Panagiotis Plotas , Dousdampanis Periklis , Kouni Sophia N. , Tsigkas Grigorios , Patsouras Nicholas Patsouras , Calogiuri GianFranco , Pourmasumi Soheila , Koniari Ioanna
Published: January 2025

This narrative review explains the history of anaphylactic or hypersensitivity reactions, their connection to the cardiovascular system, and the Kounis syndrome, which is linked to hypersensitivity. Additional subjects discussed include immunoglobulin E and serum tryptase, common pathways of allergic and nonallergic cardiovascular events, current perspectives on Kounis syndrome, allergic myocardial infarction, allergic angina, and the impact of COVID-19 and its vaccination on Kounis syndrome. Kounis syndrome is a distinct kind of acute vascular disease that affects the coronary, cerebral, mesenteric, peripheral, and venous systems. Kounis syndrome is currently used to describe coronary symptoms linked to disorders involving mast cell activation and inflammatory cell interactions, such as those involving T-lymphocytes and macrophages, which further induce allergic, hypersensitive, anaphylactic, or anaphylactic insults. Platelet activating factor, histamine, neutral proteases like tryptase and chymase, arachidonic acid products, and a range of cytokines and chemokines released during the activation process are among the inflammatory mediators that cause it. Proinflammatory cytokines are primarily produced by mast cells in COVID-19 infections. Mast cell-derived proteases and eosinophil-associated mediators are also more prevalent in the lung tissues and sera of COVID-19 patients. Virus-associated molecular patterns can activate mast cells, but allergic triggers are typically the cause. By activating SARS-CoV-2 and other toll-like receptors, a variety of proinflammatory mediators, including IL-6 and IL-1β, are released, potentially contributing to the pathology of COVID-19.
Prevalencia de síndrome depresivo en pacientes con tuberculosis resistente en el Centro materno infantil Ollantay de enero a diciembre del 2024
Authors:   , Vargas Quezada Juan Juvencio
Published: January 2025

La tuberculosis (TB) es caracteriza como una enfermedad granulomatosa crónica, siendo el vector el Mycobacterium Tuberculosis o bacilo de Koch, la detección temprana y tratamiento eficaz posibilita la recuperación de muchos individuos. No obstante, se convierte en un problema de salud debido a retrasos en el tratamiento, la resistencia a los medicamentos antituberculosos, con consecuencias clínicas y psicológicas.
Después del VIH es la segunda fuente de mortalidad por patologías infecciosas en el mundo. La OMS, afirma que la cuarta parte de la población mundial está infectada con la bacteria de la tuberculosis. En el Perú en el año 2022 se registró 19 978 mil casos de nuevos de TB. Un estudio en Perú revelo que el 37% de los individuos con el diagnóstico de TB experimentaron un síndrome depresivo.
Objetivo: Determinar la prevalencia de síndrome depresivo en pacientes con TB resistente en el Centro Materno Infantil Ollantay de enero a diciembre del 2024.
Métodos: Estudio descriptivo, prospectivo que se llevara a cabo entre enero a diciembre del 2024 con una población de pacientes con TB resistente, la muestra se extraerá del total de los expedientes clínicos que incluyan los criterios seleccionados y se llevara a cabo el cálculo del tamaño de la muestra usando el software estadístico OpenEpi. Versión, siendo un estudio no probabilístico en pacientes con TB resistente del Centro Materno Infantil Ollantay, es necesario consentimiento informado
Implementación de un Contact Center para mejorar los indicadores en una cadena de restaurantes en Lima Metropolitana, 2023
Authors:   , Santivañez Sajami Luis Enrique , Linares Aliaga Richard Alfredo
Published: January 2025

La investigación tuvo como objetivo general implementar un Contact Center para mejorar los indicadores del proceso de ventas de una cadena de restaurantes en Lima Metropolitana, 2023, justificada a través de la relevancia que tienen los indicadores del negocio, lo cual conlleva a que las empresas realicen innovaciones digitales e implementen nuevos sistemas que permitan el crecimiento y sostenibilidad a través del aumento de las ventas, satisfacción del cliente, estandarización de procesos y mejora de niveles de control, entre otros. La metodología fue de enfoque cuantitativo, diseño experimental, aplicada, para una población de 26 agentes que trabajan en el negocio. Los instrumentos utilizados tuvieron un coeficiente de correlación para la prueba de confiabilidad Test y Re Test para las ventas de 0.954 y para el tiempo medio de operación 0.986, lo cual indica una alta confiabilidad de ambos instrumentos. A través de la prueba de hipótesis usando la prueba de U de Mann Whitney se validaron los resultados del Pre Test y Post Test obteniendo un pvalor < 0.001 para las hipótesis específicas con lo cual se aceptó la hipótesis alterna que la implementación de un Contact Center sí mejora los indicadores del proceso de ventas de una cadena de restaurantes en Lima Metropolitana. Las limitaciones fueron de tamaño de la muestra, el acceso y levantamiento de información del negocio, la cantidad limitada de investigaciones en post grado sobre este tema lo que dificulta una comparación directa con otras investigaciones relacionadas a implementaciones porque las investigaciones no experimentales son las más empleadas que las experimentales.
Counterintuitive method improves yields of isotopically labeled proteins expressed in flask-cultured Escherichia coli
Authors: Treviño Miguel Ángel
Published: January 2025

NMR is a powerful tool for the structural and dynamic study of proteins. One of the necessary conditions for the study of these proteins is their isotopic labeling with 13C, 15N and sometimes 2H. One of the most widely used methods to obtain these labeled proteins is heterologous expression of the proteins in E. coli using 13C-D-glucose and 15NH4Cl as the sole nutrient sources. In recent years, the price of 13C-D-glucose has almost tripled, making it essential to develop labeling methods that are as cost effective as possible. In this work, different parameters were studied to achieve the most rational use of 13C-D-glucose, and an optimized method was developed to obtain labeled proteins with high labeling and low 13C-D-glucose consumption. Surprisingly, the optimized method is also simple and does not require monitoring of culture growth.
Shedding suppresses receptor-ligand trogocytosis to enhances long-term antitumor potential
Authors: Minagawa Atsutaka , Ishi Tomoko , Kaneko Shin
Published: January 2025

Trogocytosis is a curious phenomenon in which the rapid transfer of cell-membrane proteins between cells is induced by receptor-antigen ligation. In Chimeric antigen receptors therapy, the target antigen is transferred to T cells via trogocytosis, thereby decreasing the target density on tumor cells and abating the T cell activity. The result is tumor immune escape and T cell fratricide, two factors known to cause relapse after Chimeric antigen receptors therapy. Here we report receptor shedding as a mechanism to suppress trogocytosis. The shedding structure of Chimeric antigen receptors corresponded with the trogocytosis and long-term antitumor effect. This link between trogocytosis and shedding indicates a previously unknown form of communication between proximal cells.
Stranded assets in EU+UK agriculture during food system transformations
Authors: Kortleve Anniek , Mogollón José , Harwatt Helen , Bruckner Martin , Liu Baoxiao , Behrens Paul
Published: January 2025

A large-scale food system transformation is essential in mitigating a host of environmental crises, including climate change. Dietary shifts, particularly a reduction in animal-sourced foods (ASFs) in high-income countries, are a key pillar. These shifts risk stranding substantial ASF-related assets. ASF-focussed assets represent 78% of EU27+UK fixed agricultural assets, with €158 billion linked to livestock and €100 billion to feed production. We estimate that reductions in ASFs of 9.5%, 60%, and 100% in dietary transitions could strand 18%, 50%, and 77% of these assets, respectively. We find that there is generally sufficient time to depreciate and phase-out assets, offering a pathway for limiting stranded assets. Investors should account for potential stranding risks in financial modelling, alongside climate-related risks. Given food producers’ high exposure to the risks of asset stranding – with potential cascading effects throughout the supply chain – integrated policy support is essential in ensuring a just, effective transition.
Electromagnetic performance analysis of coaxial magnetic gear with three rotors featuring a monotonic magnetic ring and dual modulation enhancement effect
Authors: Wang Jungang , Zhang Chuntao , Mo Ruina , Ren Jiwen , Li Song
Published: January 2025

The strength of magnetic field modulation effect of coaxial magnetic gears is a key factor to measure the transmission performance of coaxial magnetic gears. Although the dual modulation coaxial magnetic gears with double modulating magnetic rings can effectively enhance the magnetic field modulation effect, there are still the problems of low torque density and serious magnetic leakage loss. In order to address the above problems, this paper proposes a three-rotor single-regulating ring dual-modulated coaxial magnetic gear that simultaneously adopts the “sum modulation effect” and “differential modulation effect”, and analyzes its working principle and the structure of multiple magnetic circuits that inhibit magnetic saturation; establishes the corresponding finite element model and analyzes its electromagnetic performance. The finite element model is established and its electromagnetic performance is simulated and analyzed; the results show that the modulation effect of the single-regulating magnetic ring double-modulated coaxial magnetic gear is strong, but the steady-state torque fluctuation of the outer rotor is large. To address this problem, Halbach magnetization is used to suppress the large fluctuations in the torque of the outer rotor, and the torque fluctuation of the outer rotor is reduced by 5.67% after the improvement. The single-modulating magnetic ring dual modulation coaxial three-rotor magnetic gear proposed in this paper enhances the magnetic field modulation effect while suppressing the magnetic saturation phenomenon, improves the utilization rate of permanent magnets, reduces the amount of modulating ring, and provides a reference for the research of single-modulating magnetic ring dual modulation coaxial magnetic gear.
TLR-5 influences T cell activity: implications for development of Type 1 Diabetes
Authors: Buschard Karsten , Krogvold Lars , Gerling Ivan , Dahl-Jørgensen Knut , Pociot Flemming , Hansen Camilla Hartmann Friis
Published: January 2025

In mammalian and human life, one of the most important problems is to choose the best defence against microorganisms. Innate cells are good against bacteria, and T cells against virus mainly because of antibody production via T helper and B lymphocytes. Toll-like receptor 5 (TLR-5) is a regulator of this choice; when it is highly expressed, T cells are inhibited, and innate cells are favored. In activated pancreatic beta cells, TLR-5 has been found highly expressed and may therefore be protected from T cell destruction e.g., during pregnancy. We investigated mRNA from islets of Langerhans from newly diagnosed T1D patients for TLR-5. Also, we examined for polymorphisms between TLR-5, metabolic parameters, and Type 1 Diabetes (T1D). mRNA for TLR-5 was downregulated by one-third in the newly diagnosed T1D patients compared to controls. Regarding polymorphisms, two associations were found between TLR-5 and monocytes which are cells from the innate immune system. Also, a significant polymorphism was seen concerning TLR-5 and T1D. T cells are important for autoimmune diseases including T1D. In the present study we find low values of mRNA of TLR-5 which enhance T cells. We hope that the findings mentioned in this article may be influential for the understanding of how T1D develops.
LLM-based Intermediate Interpretations for Predicting Nurse Stress and Its Causes from Step Count Data
Authors: Fukazawa Yusuke , Kodaka Megumi
Published: January 2025

Purpose: This study aims to propose and evaluate a hybrid approach that combines Large Language Model (LLM)-based interpretation with machine learning (ML)-based prediction to estimate stress levels and their causes from step count data. The research addresses the gap in existing datasets that lack information on the underlying causes of stress.
Methods: Step count data, stress assessments (QIDS score), and stress causes were collected from 30 nurses using six questionnaires rated on a 4-point Likert scale over eight days within one month. Two tasks were designed for evaluation: (1) assessing the contribution of intermediate interpretations to the accuracy of stress presence estimation and (2) validating the use of intermediate interpretations for identifying stress causes. BERT models with and without intermediate stress interpretations were compared, alongside a prompt-based approach.
Results: In the first task, BERT with intermediate interpretations achieved the highest accuracy of 0.74, outperforming BERT using raw step count data (0.63) and distance data (0.59). In the second task, the same model achieved a ranking accuracy of 0.60, compared to 0.56 for raw step count data. Significant correlations were identified between stress levels and work-related factors such as ''workplace relationships,'' ''busy work,'' ''heavy responsibilities,'' and ''lack of time off.''
Conclusion: The findings demonstrate that incorporating LLM-based intermediate interpretations enhances the accuracy of stress prediction and facilitates the identification of key stress causes. This hybrid approach offers a promising framework for analyzing mental health using sensor data.
An Unsuspected Case of Hirschsprung Disease in an Adolescent: a case report
Authors: Gebreziher Yirgalem , Tadesse Feven , gebre Berihu
Published: January 2025

Introduction: Hirschsprung's disease, a congenital disorder, is characterized by the absence of ganglion cells in the Meissner's plexus of the submucosa and Auerbach's plexus of the muscularis. Notably, late presentation is uncommon, with 95% of cases being diagnosed in infants younger than one year of age.
Case presentation: 15-year-old Black female patient presented to our hospital with the compliant of failure to pass feces of 5 days duration associated with abdominal distention. From an early age, she experienced persistent constipation and abdominal distention, leading to multiple admissions to local health centers for enema treatment. Oral laxatives were also prescribed, but they only provided temporary relief of her symptoms. Abdominal x-ray showed significantly dilated bowel loops with fecal loading. Barium enema showed a transition zone at the proximal one-third of the rectum with reversed rectosigmoid ratio. Rectal biopsy confirmed the diagnosis. Decompressive colostomy was done followed by soave pullthrough 3 months later.
Conclusion: Adolescent patients with a history of childhood-onset constipation should be evaluated for the possibility of undiagnosed Hirschsprung's disease. Treating this condition can significantly enhance the patient's quality of life.
Harnessing electro-generated excitons for tunable lanthanide nanocrystal electroluminescence
Authors: Liu Xiaogang , Tan Jing , Song Xiaoqing , Han Chunmiao , Wang Feng , Zhang Jing , Duan Chunbo , Han Sanyang , Xu Hui
Published: January 2025

Lanthanide nanocrystals are promising candidates for electrically powered light-emitting diodes (LEDs) due to their narrow-band emission, high color purity, and broad color turnability. However, their insulating nature poses a challenge for carrier transport and injection, impeding their application in optoelectronic devices. Here we demonstrate efficient electroluminescence from insulating lanthanide fluoride nanocrystals (5 nm; NaGdF4:Tb3+ or NaGdF4:Eu3+) coated with a series of functionalized 2-(diphenylphosphoryl)benzoic acids (ArPPOA). These ligands, featuring donor-phosphine oxide acceptor hybrids with carboxyl and P=O coordination sites, effectively sensitize the luminescence of lanthanide nanocrystals. By modulating the intensity of intra-ligand charge transfer, the first singlet (S1) and triplet (T1) energy levels of ArPPOA are precisely adjusted to optimize intersystem crossing (ISC), reverse ISC, and the interplay between singlet Förster resonance energy transfer and triplet Dexter energy transfer to the nanocrystals. Through careful control of dopant composition and concentration in nanocrystals, we also achieve wide-ranging multicolor electroluminescence without altering the device architecture, reaching an external quantum efficiency exceeding 5% for Tb3+. This ligand-functionalized nanocrystal platform provides a versatile and efficient approach for realizing wide-band tunable electroluminescent emissions.
Large-Scale Crossbar Arrays based on Three-Terminal MoS2-based Memtransistors
Authors: Das Saptarshi , Schranghamer Thomas , Pannone Andrew , Chen Chen , McKnight Thomas , Tadekawa Sean , Haines Evan , Ordonez Richard Ordonez , Hayashi Cody , Redwing Joan
Published: January 2025

Memristive crossbar architectures are promising as efficient, low-power inference engines for edge AI applications. However, inputs with minor differences often yield similar outputs, requiring additional processing methods such as confidence scoring, feedback mechanisms, crossbar redundancy, or hybrid analog-digital approaches to resolve. These methods can be impractical for resource-limited edge devices. In contrast, three-terminal memtransistors can dynamically tune conductance via gate control, effectively resolving similar outputs and enhancing separability without retraining. Here, we present dense, large-scale crossbar array architectures incorporating up to 2048 MoS2 memtransistors per array, achieving >92% yield across multiple arrays. These architectures demonstrate the ability to resolve inference ambiguities through gate modulation without the need for costly retraining or reprogramming. We also validated their performance by successfully classifying handwritten digits from the MNIST database. Additionally, these memtransistors exhibit write energies as low as ~0.2 fJ, maintain read margins up to 10⁵, and offer long-term non-volatile retention exceeding three years. To the best of our knowledge this is the largest array-level demonstration based on 2D materials reported thus far in literature. Additionally, we benchmark the performance of MoS2 memtransistors against other 2D material-based architectures and project their potential compared to state-of-the-art AI accelerators.
A Case Report of Successful Pregnancy Following Salpingo-Uterine Body Transplantation
Authors: Yang Lin , Lin Xiaoqian , Abdah Md   , Habibah Abdul Hamid 
Published: January 2025

Background
Proximal fallopian tube obstruction is a major cause of infertility in women. In cases where tubal surgery fails, assisted reproductive technologies (ART) such as in vitro fertilization (IVF) may be considered. However, a novel surgical procedure for addressing proximal fallopian tube obstruction via salpingo-uterine body transplantation is reported here.
Case Presentation:
A 35-year-old woman with bilateral proximal fallopian tube obstruction underwent a unique surgical procedure designed to restore fertility. The procedure involved the insertion of a double J stent through the vagina into the uterine cavity, with the creation of a new channel connecting the stent to the isthmus of the fallopian tube. Two months after the surgery, the patient successfully conceived and delivered a healthy boy via cesarean section. Post-delivery follow-up confirmed patency in the right fallopian tube.
Conclusion
This case demonstrates that salpingo-uterine body transplantation is a potential alternative treatment for proximal tubal obstruction, especially for patients who cannot afford IVF or wish to avoid ART. Further research is necessary to establish its broader clinical applicability.
In-situ Raman Investigation on the Phase Transition of Grunerite at High Pressure
Authors: Zheng Zhi , Zou Shaohao , Wu Chuanjun , Mei Shenghua
Published: January 2025

Amphiboles are key rock-forming minerals and play a significant role in the subduction process. Grunerite (Mg,Fe)7Si8O22(OH)2, as an important ferromagnesian amphibole, is known to undergo a phase transition from monoclinic C2/m to P21/m structure at elevated pressures. This study investigates the phase transition of grunerite under high pressure using a diamond anvil cell combined with in-situ Raman spectroscopy, aiming to identify pressure-induced phase transitions and associated structural changes. We have identified mode splits of the OH stretching modes indicative of the C2/m to P21/m phase transition at ∼1.46 GPa and observed another possible phase transition suggested by discontinuity in the OH modes at ∼17.81 GPa. The splitting of the OH stretching modes resulted from the distinct environments of two OH positions in the P21/m phase, in comparison to the crystallographically identical OH positions in the C2/m phase. We have also identified additional M–O modes in the lower-wavenumber spectrum range. The results provide new insights into the structural stability of grunerite under extreme geological conditions, contributing to a deeper understanding of metamorphic petrology and geodynamics.
Diagnóstico microbiológico e parasitológico em alface (lactuca Sativa l.) Comercializada em município do semiárido brasileiro
Authors:   , Batista João Inácio Lopes
Published: January 2025

A alface (Lactuca sativa L.) destaca-se como a folhosa mais popular e consumida no Brasil,
apresentando importância em saúde pública tanto pelo seu aporte nutricional, como pela
possibilidade de transmissão de doenças, uma vez que é comumente consumida sem cocção e
pode estar contaminada por parasitos e/ou micro-organismos patogênicos. Erros durante a
manipulação e a contaminação ambiental ocasionada, por exemplo, pela falta de saneamento
básico podem contribuir para a disseminação das doenças transmitidas por alimentos,
evidenciando a importância social da investigação parasitária e microbiana, que podem
fornecer dados sobre as condições higiênico-sanitárias envolvidas na produção,
armazenamento, transporte e manuseio dos alimentos. Neste contexto, o objetivo desse
trabalho foi diagnosticar a qualidade microbiana e parasitária de alfaces comercializadas no
município de Mossoró, Estado do Rio Grande do Norte. O estudo foi conduzido com amostras
coletadas por compra direta em bancas de feira livre e supermercados da cidade. Para a
análise microbiológica foi aplicada a técnica de Número Mais Provável (NMP) para
determinação de coliformes totais e coliformes termotolerantes, além de verificada a presença
Salmonella sp. Já para a análise parasitológica, foram utilizados o método de sedimentação
espontânea de Hoffman com modificações, e o método de centrífugo-flutuação de Faust, A
análise estatística foi executada aplicando o teste χ2 e teste exato de Fisher, com nível de
significância de 5%. Para as análises microbiológicas foram analisadas uma amostra por
estabelecimento, onde verificou-se que 100% (12/12) apresentaram contaminação por
coliformes totais, 25% (3/12) coliformes termotolerantes acima do permitido e ausência de
Salmonella sp. Quanto a contaminação parasitária, 62,5% (45/72) estavam contaminadas,
sendo diagnosticado os parasitos: ancilostomídeos 40,3% (29/72), Strongyloides sp. 29,2%
(21/72), Ascaris sp. 1,4% (1/72), Entamoeba histolytica/díspar 1,4% (1/72), Endolemax nana
1,4% (1/72) e Entamoeba coli 2,8% (2/72). Outro achado de relevância foi a contaminação
física com o diagnóstico de insetos em 19,4% (14/72), além de larvas de Oesophagostomum
sp. 16,7% (12/72) e outras larvas de nematoides de vida livre sem importância em saúde
pública. Entretanto não houve diferença na qualidade parasitária e microbiana entre os locais
de comercialização deste estudo. Assim, fica evidenciada a presença de contaminação
microbiana e parasitária, determinando a baixa qualidade higiênico-sanitária das alfaces
estudadas e o potencial risco a saúde ao qual a população fica exposta.
Modo de cuidar do lixo eletroeletrônico: analisando mudanças na ação dos estudantes em oficinas ambientais na escola de ensino médio Lauro Rebouças de Oliveira em Limoeiro do Norte-CE
Authors:   , Castro Maria do Socorro Santos de Assis
Published: January 2025

Essa pesquisa examina juntamente com estudantes da escola pública estadual Lauro Rebouças de Oliveira de Limoeiro do Norte-Ce, as mudanças nas coordenações de ações em uma experiência de educação ambiental focada no descarte e tratamento do lixo eletroeletrônico na escola. O lixo eletroeletrônico cresce desordenadamente a cada dia como consequência das mudanças em nossa sociedade, quando as relações ser humano – objeto técnico acontecem em meio à integração de tecnologias digitais cada vez mais potentes nas diversas ações cotidianas. Trata-se de um lixo especialmente perigoso, tóxico e, portanto, nocivo ao meio ambiente e à saúde humana, por isso é crucial focarmos a pesquisa intervenção em educação ambiental na questão do e-lixo, porque envolve a relação homem-natureza que é essencial para o bem-estar de toda humanidade. (SOUSA, 2009). Esse olhar diferenciado que ora lançamos para essa realidade nos faz perceber o mundo como um todo integrado, onde os sujeitos reconhecem-se como parte do meio ambiente. A rede teórica de sustentação da pesquisa encontra apoio nos estudos de Tundisi (1997) e Carvalho (1991), construções sobre a problemática do lixo e da educação ambiental; e Simondon (1989), filósofo e físico, um clássico que nos ajuda a situar como acontece a relação entre os processos de individuação humana, técnica e física na configuração do conjunto que designamos como sociedade. O objetivo central da pesquisa é analisar as coordenações de ações de estudantes de uma escola pública de ensino médio sobre uma experiência de recolhimento do eletroeletrônico e aprofundamento do estudo sobre a destinação desse lixo no município de Limoeiro do Norte.
A metodologia escolhida para o desenvolvimento é a pesquisa-intervenção de natureza qualitativa e empregaremos o método do percurso comentado Jean Paul Thibaud (2004) para distinguir e avaliar as ações e transformações realizadas pelo coletivo dos estudantes engajados na pesquisa no decorrer do percurso. Esta metodologia permite analisar o ambiente através da observação e recorrências e/ou diferenças identificadas em excertos das oficinas e as transcrições de comentários do percurso, referidos à experiência direta dos estudantes nos momentos em que se dedicam ao projeto em andamento na escola de coleta e encaminhamento do lixo eletroeletrônico. Como resultado da pesquisa, pudemos distinguir mudanças nos modos de perceber a problemática do lixo eletroeletrônico na experiência dos estudantes, saberes e conhecimentos que podem contribuir com avanços na experiência da Educação Ambiental voltada a problemas contemporâneos, produzir mudanças nas ações dirigidas ao tratamento do lixo eletroeletrônico na experiência dos estudantes na escola e, ainda, no fortalecimento de políticas públicas direcionadas ao tratamento de resíduos eletrônicos, a partir da experiência de um grupo de estudantes de uma escola de ensino médio de Limoeiro do Norte.
Ciências ambientais e interdisciplinaridade: estudo bibliométrico de teses em programas 6 e 7
Authors:   , Cavalcanti Daniele Belmont de Farias
Published: January 2025

A Área de Ciências Ambientais (CiAmb) na CAPES foi criada em 2011, como resposta para a complexidade dos problemas ambientais, face à indissociabilidade entre sistemas antrópicos e naturais, por isso constitui um campo de pesquisa aglutinador de inúmeras disciplinas que tem na Interdisciplinaridade o significado da sua existência. Foi a partir dessa discussão que nasceu a motivação para realização dessa pesquisa: as teses defendidas em Programas de Pós-graduação inseridos na subárea CiAmb apresentam as características interdisciplinares previstas no Documento da Área? Desse questionamento nasceu o objetivo geral dessa pesquisa: analisar a Interdisciplinaridade nas teses dos programas CAPES 6 e 7 defendidas entre 2011 a 2017, considerando as características descritas no ―Documento da Área: Ciências Ambientais‖. Esse trabalho fez uso dos métodos bibliométricos e cientométricos para a análise da interdisciplinaridade e criação dos indicadores bibliométricos e de Interdisciplinaridade. Os dados coletados nos resumos das 322 teses foram organizados e tabulados no software MS Office Excel 2007, a fim de subsidiar a análise descritiva e no programa Word Cloud Generator para criação da nuvem de palavras. Os resultados demonstraram que os aspectos de Interdisciplinaridade para a formação da equipe, temas e integração ambiental, social e tecnológico, exigidos para a área de Ciências Ambientais, estão presentes nas teses dos programas de pós-graduação CAPES 6 e 7, apesar de certa concentração dos temas e formações de egressos, orientadores e coorientadores em determinadas instituições, bem como as teses devem contemplar mais o uso das tecnologias em suas pesquisas, para melhorar o indicador interdisciplinar de integração ambiente-social-tecnologia. Esse trabalho teve como contribuição fornecer subsídios para a gestão mais eficiente dos programas CAPES 6 e 7, como também veio colaborar para que outros programas inseridos na CiAmb tenham desempenho semelhante a esses programas no que tange a avaliação das características interdisciplinares, à medida que constatou existir uma evidente interdisciplinaridade nas teses da área de Ciências Ambientais, aspecto fundamental e base sobre a qual essa área foi alicerçada, bem como identificou algumas oportunidades de melhorias em alguns aspectos como desenvolvimento de pesquisas que integrem mais as questões tecnológicas, de forma a assegurar que os objetivos que deram origem a área de Ciências Ambientais estão sendo alcançados na plenitude e de forma excelente.
Identification of Key Genes and Pathways in Lenvatinib-resistant Hepatocellular Carcinoma using Bioinformatic Analysis and Experimental Validation
Authors: Yang Ming , Wang Zhaoyue , Su Riga , Li Dongbing , Zhou Jun
Published: January 2025

Background:

Resistance to lenvatinib poses a serious threat to the therapy of patients
with Hepatocellular Carcinoma (HCC). The mechanism by which HCC develops resistance
to lenvatinib is currently unknown.

Objective:

The aim of this study was to identify key genes and pathways involved in lenvatinib
resistance in HCC using bioinformatic analysis and experimental validation.

Methods:

Differentially expressed genes (DEGs) were identified from the GSE186191 gene
expression profile, comparing HCC cell lines with lenvatinib-resistant HCC cell lines. Gene
Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were then carried out using
DAVID. A protein-protein interaction network was constructed to visualize DEGs and
identify hub genes. The expression and prognostic significance of these hub genes were further
examined. Additionally, genomic enrichment analysis (GSEA) was utilized to investigate
the potential functions of key genes. Following this, the presence of AHSG was validated
in both the original Huh7 cells and the lenvatinib-resistant Huh7 (Huh7LR) cells resistant
to lenvatinib through the utilization of quantitative real-time PCR (qRT-PCR).

Results:

A total of 232 DEGs were identified between HCC cell lines and those that are resistant
to lenvatinib. These DEGs were significantly associated with arrhythmogenic right
ventricular cardiomyopathy, hypertrophic cardiomyopathy, dilated cardiomyopathy, and
mucin-type O-glycan biosynthesis. Three hub genes, including AHSG, C6, and ORM1,
were identified. The low expression of AHSG showed a poorer prognosis in HCC. GSEA
demonstrated a significant correlation between low AHSG expression and pathways involving
fatty acid metabolism, ribosome function, glycine, serine, and threonine metabolism, peroxisome
activity, and bile acid biosynthesis. The expression of AHSG was notably reduced
in Huh7LR cells (p = 0.006) compared to Huh7 cells.

Conclusion:

Diminished AHSG expression is strongly associated with lenvatinib resistance
in HCC, suggesting that it may have implications for developing effective strategies to overcome
this resistance.
FOLR1 Regulates the Malignant Progression of Glioblastoma through the SRC/ERK1/2 Axis
Authors: Jia Xueshan , Liang Weihang , Yang Junya , Chen Xuejiao , Bin Yi , Cao Zhikun , Tian Qingfeng
Published: January 2025

Background:

GBM is an aggressive brain tumor with limited treatment options. Prior
research has indicated FOLR1 as a pivotal gene involved in cancer pathogenesis.

Aim:

This study aimed to explore the involvement of folate receptor alpha (FOLR1) in glioblastoma
(GBM) and evaluate its potential as a therapeutic target.

Objective:

This study investigated the expression pattern of FOLR1 in GBM, its impact on patient
prognosis, and its role in GBM cell growth and the SRC/ERK1/2 signaling axis.

Methods:

Initially, we conducted an expression analysis of FOLR1 based on public databases
and examined its expression pattern in GBM and its impact on patient prognosis. Subsequently,
cell experiments were carried out to evaluate the regulation of GBM cells by differential FOLR1
expression. We then downloaded 100 FOLR1 co-expressed genes from the Linkedomics data
repository and performed an enrichment analysis. Finally, the role of FOLR1 and SRC/ERK1/2
axis in GBM was analyzed again by cell experiments.

Results:

FOLR1 was found to be substantially expressed in GBM patients and was linked to a
poor prognosis. Cell experiments showed that overexpression of FOLR1 promoted GBM cell
growth, while low expression of FOLR1 inhibited cell growth. Additionally, genes related to
FOLR1 were enriched in the lysosome, toxoplasmosis, and other pathways. This study further
indicated that FOLR1 facilitates the activation of the SRC/ERK1/2 signaling pathway in GBM
cells, and the attenuation of these pathways can effectively impede the malignancy-promoting
effects triggered by FOLR1 in GBM cells.

Conclusions:

We revealed that FOLR1 orchestrates the malignant advancement of GBM by
stimulating the SRC/ERK1/2 signaling axis, underscoring its pivotal role in the pathogenesis of
GBM.

other:

/
Niosomal Encapsulation of Anti-Cancer Peptides: A Revolutionary Strategy in Cancer Therapy
Authors: Patel Bhaveshkumar A. , Vashi Ankur , Borra Ramakrishna , Patel Mehul
Published: January 2025

Cancer treatment has evolved significantly over the years, incorporating a range of
modalities including surgery, radiation, chemotherapy, and immunotherapy. However, challenges
such as drug resistance, systemic toxicity, and poor targeting necessitate innovative approaches.
Peptides have gained attention in cancer therapy due to their specificity, potency, and
ability to modulate various biological pathways. Peptide-based drugs can act as hormones, enzyme
inhibitors, or targeting ligands, contributing to their versatile role in cancer treatment.
However, peptides face several challenges, including instability, rapid degradation, and poor
bioavailability. One promising strategy is the use of niosomal delivery systems for peptidebased
therapies. Niosomes, which resemble liposomes in structure, are vesicles based on nonionic
surfactants. They are composed of a bilayer created through the self-assembly of non-ionic
surfactants in water, enabling them to encapsulate hydrophilic, lipophilic, and amphiphilic
drugs. Their unique properties, such as biocompatibility, biodegradability, and ability to encapsulate
diverse therapeutic agents, make them suitable for drug delivery applications. This review
aims to explore how the niosomal preparation of peptides can revolutionize oncology drugs by
overcoming critical challenges like drug resistance, systemic toxicity, poor targeting, instability,
rapid degradation, and low bioavailability. This review aims to explore how niosomes can specifically
address key limitations in cancer therapy, including targeting, bioavailability, and stability
of peptide-based drugs. By consolidating recent advancements, the review sheds light on
how niosomal encapsulation can overcome barriers in cancer treatment and improve therapeutic
outcomes for patients.
Dual Biological Effects and Mechanisms of Gut Microbiota in Breast Cancer: A Review
Authors: Ma Long , Liu Yuxin , Ning Mingliang , Ding Xiaoyun , Shang Chunyan , Mai Bin , Hu Zhiqiang , Zhou Ru
Published: January 2025

Breast cancer (BC) is a common malignant tumor. BC is serious and has a high
mortality rate. The incidence of BC has increased in recent years. The incidence rate is higher
in coastal cities than in inland areas and in urban areas than in rural areas. Affect the occurrence
and development of BC, such as inflammation and immune response. One of the cutting-
edge studies to gain insight into the pathogenesis of BC is through the gut microbiota
(GM) influencing the health status of the human body. Advances in metabolomics analysis
can provide a clearer picture of changes in the composition of GM. The composition and
quantity of GM can affect the health of other parts of the body, and the disorder of GM can
lead to the occurrence and aggravation of diseases in different parts of the body. This paper
not only discusses the dual biological effects of GM on BC, the relationship between GM and
its metabolites, and BC and its related mechanisms but also explores traditional treatments for
BC as well as the treatment of BC through GM, providing theoretical support for the use of
methods to regulate GM in the clinical study of BC.
The Hormetic Potential of GDF15 in Skeletal Muscle Health and Regeneration: A Comprehensive Systematic Review
Authors: Tarchi Livio , Maiolini Gaia , Villa Gianluca , Rovero Paolo , Logu Francesco De , Nassini Romina , Garella Rachele , Sassoli Chiara , Ricca Valdo , Castellini Giovanni , Squecco Roberta
Published: January 2025

Background:

Growth Differentiation Factor 15 (GDF15) has been described as
influencing skeletal physiology. Nevertheless, no systematic appraisal of the effect of GDF15 on
skeletal muscle tissues has been developed to the present day.

Objective:

The aim of the present work was to review the evidence on the topic.

Methods:

In this preregistered systematic review (https://osf.io/wa8xr), articles were retrieved
from MEDLINE/PubMed, EMBASE, and WebOfScience. Inclusion criteria comprised studies on
humans or animal models, assessment of peripheral or local tissue GDF15 concentrations, as
well as the direct expression of GDF15 in skeletal muscle, and direct or indirect correlates of
GDF15 with physical activity/ sarcopenia/trophism/ function.

Results:

A total of 646 studies were retrieved, and 144 finally included. Molecular inducers or
inhibitors of GDF15 in skeletal muscle tissues were described. GDF15 was reported to promote
skeletal muscle health, metabolic homeostasis, and overall physical conditioning. In pathology,
GDF15 seems to be correlated to the degree of muscle impairment and mitochondrial stress.
GDF15 has also been described as having the potential to stratify patients based on clinical
prognosis and functional outcome.

Conclusion:

A hormetic hypothesis for GDF15 on skeletal muscle was proposed. In fact, GDF15
exhibited beneficial effects when expressed at high levels facing acute stressors (i.e.,
“myoprotection”). Conversely, GDF15 exhibited maladaptive effects, such as chronic low-grade
inflammation, when chronically expressed in pathological processes (e.g., obesity, aging). GDF15
may be a potential molecular target for disease-modifying interventions. The current review
underscores the need for further research on GDF15 to elucidate its therapeutic potential across
different pathological states.
The study protocol, registered before data collection and analysis, can be retrieved at
https://osf.io/wa8xr. It should be noted that the study deviated from the protocol after peer review,
including other electronic databases beyond MEDLINE/PubMed alone.
Elucidating the Causal Dynamics between Inflammatory Proteins
and Atrial Fibrillation Risk Through Bidirectional Mendelian
Randomization
Authors: Lv Yuan , Huang Bin , Xu Liyin , Wu Xianjun
Published: January 2025

Background:

Atrial fibrillation (AF), the most common cardiac arrhythmia, is
associated with significant morbidity and mortality. Inflammation has been implicated in
the pathogenesis of AF, but the causal relationship between specific inflammatory
proteins and AF risk is not well established. This study aims to clarify this relationship
using a bidirectional two-sample Mendelian Randomization (TSMR) approa

Methods:

Employing a bidirectional Mendelian Randomization (MR) method, we
analyzed genetic variants as instrumental variables (IVs) to investigate the influence of
91 circulating inflammatory proteins on AF risk. This approach allowed us to assess
the potential causal effects of inflammatory proteins on AF and vice versa, thus
providing a comprehensive understanding of the bidirectional nature of their
relationship.

Results:

Seven inflammatory proteins were significantly associated with AF risk.
Three proteins increased the risk: Fibroblast Growth Factor 5 (FGF-5) with an odds
ratio (OR) of 1.0743 (95% CI: 1.0466-1.1027, p=7.41E-08), Tumor Necrosis Factor
(TNF) with an OR of 1.0832 (95% CI: 1.0261-1.1434, p=0.0038), and Interleukin-2
Receptor Subunit Beta (IL-2RB) with an OR of 1.0814 (95% CI: 1.0151-1.1519,
p=0.0153). Four proteins showed a protective effect: CD40 Ligand Receptor (CD40)
with an OR of 0.9671 (95% CI: 0.9392-0.9959, p=0.0254), Fms-related Tyrosine
Kinase 3 Ligand (FIt3L) with an OR of 0.9553 (95% CI: 0.9173-0.9949, p=0.0274),
Leukemia Inhibitory Factor Receptor (LIF-R) with an OR of 0.9254 (95% CI: 0.8678-
0.9868, p=0.0181), and Sulfotransferase 1A1 (ST1A1) with an OR of 0.9461 (95% CI:
0.9097-0.9839, p=0.0056). The reverse MR analysis revealed no significant effects of
AF on the levels of these inflammatory proteins, suggesting a unidirectional causality
from proteins to AF.

Conclusion:

This bidirectional MR study provides robust evidence for a causal
relationship between specific inflammatory proteins and AF risk. The identified proteins
could serve as potential biomarkers for AF risk stratification and targets for therapeutic
intervention, offering new insights into the pathophysiology of AF and avenues for
future research.
Integrating Forensic Autopsies with Proteomic Profiling for Suicide Risk Assessment: A Comprehensive Review of Literature
Authors: Al-Habash Ibrahim H. , Alshaeb Asma Mahmoud , Srpanova Viktorija Belakaposka , Alempijevic Djordje , Keckarevic-Markovic Milica , Concato Monica , Radaelli Davide , D’Errico Stefano
Published: January 2025

Background:

Suicide is a major global public health concern that affects people of all ages,
with over 700000 individuals intentionally ending their lives every year. Suicide is a multifactorial
event related to multiple risk factors interlocking with each other, among which neurobiological
factors are considered to be an objective measure of the incidence of this phenomenon and
can be used as a measurable tool for evaluating suicidal tendencies.

Objective:

The aim of this study is to thoroughly examine available data and assess candidate proteins
as prospective biomarkers for predicting suicides and ascertaining the manner of death in forensic cases.

Methods:

An electronic search was conducted on PubMed, Science Direct Scopus, and the Excerpta
Medica Database. The systematic review adhered to PRISMA guidelines and encompassed case series,
prospective and retrospective studies, and short communications published in English. The focus
was on proteomics and suicide, specifically, those studies where researchers conducted human
proteomic analyses on specimens obtained from individuals who completed or attempted suicide.

Results:

A total of 14 studies met the inclusion criteria, resulting in a dataset of numerous candidate
protein biomarkers. These include tenascin-C, potassium voltage-gated channel subfamily Q member
3, vimentin-immunoreactive astrocytes, glutathione S-transferase theta 1, iron transport proteins, Acrystallin
chain B, manganese superoxide dismutase, glial fibrillary acidic protein, various glycolytic
pathway proteins, 14-3-3 eta and 14-3-3 theta proteins, specific cytoskeleton proteins, C-reactive protein,
serum amyloid A protein 1, extrinsic coagulation pathway proteins, the vacuolar-type proton pump
ATPase subunit, plasma apolipoprotein A-IV, and ER stress proteins. These proteins are proposed as a
panel of biomarkers to be evaluated in conjunction with other clinical predictors of suicide.

Conclusion:

This review aims to provide a comprehensive summary of all proteomic studies conducted
on cases of attempted or completed suicide. By doing so, it seeks to bridge existing gaps in
knowledge and pave the way for future investigations. The ultimate goal is to potentially identify a
suicide biomarker.
Two Novel Peptides from Buthotus saulcyi Scorpion Venom: Proteomic
Analysis and Approaches
Authors: Zargan Jamil , Jahangirian Ehsan , Khan Haider A. , Ali Shakir
Published: January 2025

Background and Objectives:

Venomous scorpions play a crucial role in medicine and
public health. Buthotus saulcyi scorpion is known as one of the most populous species in East Asia
and Iran, while its venom proteome has still not been fully determined.

background:

In the present study, we report for the first time a proteomic profile of Buthotus saulcyi scorpion venom with the aim of looking ahead and determining the structural and functional characteristics of these compounds for use as medical tools.

Aims:

In the current research, the proteomic profile of Buthotus saulcyi scorpion venom to determine
the structural and functional characteristics of its compounds used for treatment will be examined
for the first time.

objective:

In the present study, we report for the first time a proteomic profile of Buthotus saulcyi scorpion venom with the aim of looking ahead and determining the structural and functional characteristics of these compounds for use as medical tools.

Method::

2D-PAGE, HPLC, SDS-PAGE, sequencing, and MALDI-TOF MS techniques were used
to investigate the properties of these peptides.

Result:

The 2D-PAGE analysis of crude toxin from B. saulcyi revealed a minimum of 96 protein
spots, with isoelectric points ranging from 4 to 9 and molecular weights spanning from 3.6 to 205
kDa. Following this, HPLC was used to isolate 14 fractions of crude toxins, and the protein content
of these fractions was measured. SDS-PAGE analysis identified 7 protein bands within the B. saulcyi
crude toxin fractions, with molecular weights ranging from 13 to 217 kDa. Further examination
of fraction 7 through amino acid sequencing resulted in the identification of two protein bands labeled
peptide 3 and peptide 4. Ultimately, these protein bands were extracted, and their molecular
mass and amino acid sequences were analyzed using MALDI-TOF MS.

result:

2D-PAGE data from the B. saulcyi crude toxin showed at least 96 protein spots in isoelectric point between 4 and 9 and a molecular weight between 3.6 and 205 kDa. Then 14 fractions of crude toxins were isolated using HPLC and their protein content was estimated. SDS-PAGE analysis of fractions of B. saulcyi crude toxin showed 7 protein bands with a molecular weight between 13 and 217 kDa. Subsequently, the amino acid sequencing of Fraction 7 led us to two protein bands designated as Peptide 3 and Peptide 4 peptides. Finally, these protein bands were removed, and molecular mass and amino acid sequence analysis was performed using MALDI-TOF MS.

Conclusion::

According to our results, the alignment of P3 and P4 protein sequences revealed the
highest similarity to chrysophsin 2 and pheromone-bound protein 2, respectively.