bluuebunny/crossref_metadata_2025 huggingface dataset:
New technique for direct fluoroimmunomagnetic detection of rotavirus in water samples
Authors: Villamizar-Gallardo Raquel A. , Osma Johann F. , Ortíz Oscar Orlando
Published: September 2017

A new rapid, sensitive and selective method for rotavirus detection in water samples is described in this paper. Amino pink magnetic microparticles were functionalized with monoclonal antibodies and used to capture, concentrate, separate and detect infectious rotavirus particles in distilled and drinking water samples. The fluorescence of the microparticles was used to determine the presumptive presence of rotaviruses by using confocal microscopy. Atomic force microscopy and transmission electron microscopy were used to confirm the presence of the anti-rotavirus antibodies attached to the surface of the magnetic microparticles as well as that of viruses attached through the antibody. In addition, RNA extraction, quantification and amplification were carried out to validate the microscopic observations. The selectivity of the microparticles was tested in a sample containing a mix of enteric viruses. It was concluded that functionalizing fluoromagnetic microparticles with anti-rotavirus monoclonal antibodies constituted a fast, simple and reliable technique for detecting as low as 10 Rotavirus particles in 1 L of artificial or real water in just 2 hours.
Organic matter degradation in a greywater recycling system using a multistage moving bed biofilm reactor (MBBR)
Authors: Saidi Assia , Masmoudi Khaoula , Nolde Erwin , El Amrani Btissam , Amraoui Fouad
Published: September 2017

Greywater is an important non-conventional water resource which can be treated and recycled in buildings. A decentralized greywater recycling system for 223 inhabitants started operating in 2006 in Berlin, Germany. High load greywater undergoes advanced treatment in a multistage moving bed biofilm reactor (MBBR) followed by sand filtration and UV disinfection. The treated water is used safely as service water for toilet flushing. Monitoring of the organic matter degradation was pursued to describe the degradation processes in each stage and optimize the system. Results showed that organic matter reduction was achieved for the most part in the first three reactors, whereas the highest reduction rate was observed in the third reactor in terms of COD (chemical oxygen demand), dissolved organic carbon and BOD7 (biological oxygen demand). The results also showed that the average loading rate entering the system was 3.7 kg COD/d, while the removal rate was 3.4 kg COD/d in a total bioreactor volume of 11.7 m³. In terms of BOD, the loading rate was 2.8 kg BOD/d and it was almost totally removed. This system requires little space (0.15 m²/person) and maintenance work of less than one hour per month and it shows operational stability under peak loads.
De víctimas de trata a victimarios: Los agentes facilitadores del cruce fronterizo reclutados por los cárteles mexicanos
Authors: Izcara Palacios Simón Pedro
Published: September 2017

El objetivo de este artículo es examinar qué métodos utilizan los cárteles de la droga mexicanos para reclutar a agentes facilitadores del cruce fronterizo y cuáles son las actividades que los ex agentes facilitadores del cruce fronterizo realizan para estas organizaciones. Para ello se utilizó una metodología cualitativa que incluyó la realización, entre los años 2011 y 2015, de entrevistas a 107 agentes facilitadores del cruce fronterizo (105 varones y 2 mujeres). Este artículo concluye que a partir de 2013 se ha producido un incremento del reclutamiento no forzado de agentes facilitadores del cruce fronterizo por los cárteles de las drogas mexicanos, y que las actividades que realizan son cada vez más violentas.
La variabilidad histórica de la corriente del Río Colorado. El vínculo con la minuta 319
Authors: Samaniego López Marco Antonio
Published: September 2017

El propósito de este artículo es analizar los efectos de la variabilidad histórica de la corriente del río Colorado y cómo se ha utilizado dicha variabilidad en las discusiones para el control y administración de la  mencionada cuenca internacional. Con base en el análisis de estudios realizados por diversos ingenieros, abogados y políticos estadounidenses, demostramos que desde principios del siglo XX se sabía de esta variabilidad. Específicamente se busca explicar el significado y consecuencias que tuvo el tema de la variabilidad en la minuta 319, firmada por la Comisión Internacional de Límites y Aguas (International Border and Water Commission), donde por primera ocasión se contempla de manera explícita el cambio climático, dado que diversos sectores han insistido en profundizar en el tema de definir “sequía extraordinaria”, argumentando que una cuota fija a México no tomó en cuenta el factor de cambio climático.
The Influence of the 5 A's Intervention towards Teenagers Attitude in Consuming Liquor at Kupang City
Authors: Ekawati Christine , Agustina - , Dukabaen Olga , Telan Albina Bare
Published: September 2017

Liquor was an alcoholic beverage consist of Ethanol. The liquor effects changed the teenager's behavior and could be occurred a disease that was harmful to our body. The 5 A's interventions could be conducted to change attitudes. Regarding three domains i.e. cognitive domains, affective domains and conative domains. The research type was an experiment quasi with randomized pre-test post-test control group design. The sample was taken from 20 respondents for the conventional group and 20 respondents for the 5 A's intervention group. The results stated that the 5 A's intervention group was able to change attitudes on the 3 domains those were cognitive, affective, and conative.
Financial Feasibility of Investing in Smallholder Cow-Calf Cooperatives in Baluran National Park
Authors: Rademaker Mark , Suryantini Any , Mulyo Jangkung Handoyo
Published: September 2017

Livestock grazing is a major driver of human-wildlife confl ict in conservation areas. Currently, it is estimated that 3000 heads of cattle illegally grazing within Baluran National Park (BNP) in East Java. The recent research has suggested the potential of livestock system intensifi cation to reduce land-use and conflict through conservation priorities. The research goal was to investigate the fi nancial feasibility of starting intensive cow-calf cooperatives by smallholders in the BNP area. Data were collected using Farm surveys in a Criterion sampling design. Optimal herd management plans were generated using whole farm Linear Programming and fi nancial feasibility was assessed using Discounted cash-flow analysis and debt-servicing capabilities. Investment lifetime was set at 15 years and four alternative varieties of cattle were taken from Bali, Peranakan Ongole, Limousin and Simmental. Results show that investing in all varieties represents a positive investment opportunity. Bali cattle obtaining the highest NPV ($53.769), IRR (14,25%) and B/C ratio (1,13). Farmer income can be increased by 163% by combining additional Off-farm labor. However, debt servicing capabilities of cow-calf cooperative activities showed that the loan principal can only be repaid in the 10th year instead of the maximum eight years set by the government cow-calf credit scheme. We urge the government to reconsider either the grace period or the repayment time of the credit scheme to better fit the cash-fl ow characteristics of cow-calf enterprises.
DEVELOPMENT OF MESALAZINE MICROSPHERES FOR COLON TARGETING
Authors: Rajesh Katta , Deveswaran R. , Bharath S. , Basavaraj B. V.
Published: September 2017

Objective: The present work was aimed at preparation of mesalazine microspheres by a non-aqueous solvent evaporation method using eudragit S 100 and eudragit L 100 as pH dependent polymers for colon targeting.Methods: The ratio of drug to polymer was varied and the effect of formulation variables revolutions per minute (RPM) (1000, 1500, 2000 and 2500) and concentration of span 80 (1%, 1.5%, 2% and 2.5%) were studied. Prepared microspheres were evaluated for particle size, percent drug entrapment, granular analysis, in vitro drug release studies, Fourier transformed infrared spectroscopy (FT-IR) Differential Scanning Calorimetry (DSC), X-ray diffraction (XRD) and scanning electron microscopy (SEM) studies.Results: Particle size has decreased and percent drug entrapment had increased with increase in RPM in all formulations. When the span 80 concentration increased, the particle size of the microsphere formulations increased and percent drug entrapment decreased in eudragit S 100 microspheres; whereas in eudragit L 100 microspheres, as the concentration of span 80 increased, the particle size of the microsphere formulations decreased. The prepared microspheres sustained the drug release over a period of 12 h.Conclusion: Thus eudragit S 100 and eudragit L 100 microspheres could constitute a promising approach for colon-specific and sustained delivery of mesalazine for the treatment of inflammatory bowel disease.
STUDIES ON CROSS-LINKED CHITOSAN HYDROGEL FOR MATRIX TABLETS OF MONTELUKAST SODIUM
Authors: Hebbar Srinivas , Dubey Akhilesh , S. Ravi G. , Mascarenhas Shanon Ben
Published: September 2017

Objective: The aim of the present study was to prepare hydrogel matrix tablets for controlled release of an anti-asthma drug (Montelukast sodium) by modifying the applications of chitosan by crosslinking it with the different cross linking agent.Methods: The hydrogels were prepared by crosslinking chitosan using three different crosslinking agents namely, anhydrous dextrose (DXT), sodium tripolyphosphate (TPP) and glutaraldehyde (GL). Formulations were prepared by direct compression method and pre and post compression parameters were evaluated.Results: FTIR (Fourier transform infrared spectroscopy) studies of tablet formulation indicated that there is no drug-excipient interaction in the prepared formulations. The matrix tablets were capable of releasing the drug for 11 h depending upon the formulation variables. The tablets prepared by plain chitosan discharged the drug quickly, while those prepared by using GL crosslinked-hydrogel released the drug more slowly in a controlled manner. In general, the order of drug release from the crosslinked hydrogel matrix tablets on the basis of crosslinking agents, was found to be DXT>TPP>GL. The type of cross-linking agents affected the drug release rate and in the case of the tablets prepared with CHTPP (95 % to 83 %) it was slower than for the tablets prepared with CHDX (96 % to 88 %) at the end of 11th h. CHGL tablets showed more prolonged drug release profiles (86 % to 74 %) as compared to CHDX and CHTPP at the end of 11th h. In vitro release data was fitted into various release kinetic models to study the release mechanism and showed zero order kinetics and “n” value were found to be less than 0.5 indicated the release mechanism followed fickian diffusion due to swelling of gel matrix and high solubility of montelukast sodium.Conclusion: From the experimental results it can be concluded that hydrogels of chitosan were successfully prepared by using DXT, TPP and GL with different concentration.
FORMULATION OF FAST-DISSOLVING TABLETS OF DOXAZOSIN MESYLATE DRUG BY DIRECT COMPRESSION METHOD
Authors: Preethi G. B. , Banerjee Sayan , Shivakumar H. N. , Ravi Kumar M
Published: September 2017

Objective: The rationale of the current research work was to formulate and evaluate fast-dissolving tablets of doxazosin mesylate with minimum disintegration time and improved dissolution efficiency using solid dispersion method.Methods: Solid dispersions of doxazosin mesylate and polyethylene glycol 8000 in different ratios were prepared using the kneading method. The prepared solid dispersions were subjected to drug interaction and dissolution studies to select the effective solid dispersion for the formulation of fast-dissolving tablets. Fast dissolving tablets containing drug-polyethylene glycol 8000 solid dispersion (1:3) were prepared using various super-disintegrants such as crospovidone, croscarmellose sodium, mixture and coprocessed crospovidone and croscarmellose sodium in concentration range of 2% and 5% by direct compression technique. The prepared formulations (F1–F16) were evaluated for post compression parameters; hardness, thickness, friability, wetting time, disintegration time, and in–vitro drug release.Results: Drug doxazosin mesylate showed enhanced aqueous solubility of 13.3µg/ml in the presence of polyethylene glycol 8000. Differential scanning calorimetery and Fourier transform infrared spectroscopy studies confirmed no interaction between drug and polyethylene glycol 8000and, drug-polyethylene glycol 8000 solid dispersion showed cumulative drug release of 44.48% in 60 min. Formulated FDT of drug-polyethylene glycol 8000 solid dispersion, containing coprocessed mixture of crospovidone and croscarmellose sodium (5%) exhibited disintegration time of 14.5s with percentage cumulative release of 92.46% in 60 min.Conclusion: The work reasonably concludes that for the formulated doxazosin mesylate-fast dissolving tablets, disintegration time was effectively reduced by the presence of coprocessed mixture of crospovidone and croscarmellose sodium and dissolution efficiency was improved by preparation of solid dispersion with polyethylene glycol 8000.
DEVELOPMENT AND EVALUATION OF NOVEL DRUG DELIVERY SYSTEM OF TOLTERODINE TARTRATE
Authors: Patil Vijaykumar , Belsare Deepak
Published: September 2017

Objective: Tolterodine tartrate (tolterodine) is used for treating overactive bladder (OAB) with symptoms of urinary frequency, urgency and leakage. Tolterodine is an antimuscarinic (anticholinergic) agent. It works by blocking a chemical that causes contractions of the bladder. Present work involved development of a novel drug delivery system of tolterodine intended to be taken once daily.Methods: Extended release (ER) pellets of tolterodine were prepared and optimized for in vitro drug release. Subsequently, these pellets were filled into a suitable sized capsule. The resulting capsules were evaluated for in vitro drug release. Optimized formulation was subjected to accelerated stability studies for 3 mo and was evaluated for description, average weight, assay and drug release.Results: The optimized ER capsule exhibited similar dissolution profile as that of the reference listed drug (RLD), with approximately 45%, 75% and more than 80% release in 3 h, 5 h and 7 h respectively. Accelerated stability studies indicated good physical and chemical stability of the formulation.Conclusion: ER formulation of tolterodine was optimized and can be used as once a day dosage, reducing the frequency of administration when compared with the immediate release formulation. The developed formulation exhibited similar behavior as that of reference formulation Detrol LA marketed in the US.
DEVELOPMENT, CHARACTERIZATION AND EVALUATION OF SOFT ORAL EDIBLE GEL USING GELLAN GUM
Authors: P Vijayanand , A. Deepa , M. Bhagavan Raju
Published: September 2017

Objective: The objective of present research work was to formulate and evaluate a novel oral edible gel dosage form using gellan gum as gelling agent and carvedilol as a model drug to ease the administration to dysphagic and geriatric patients.Methods: Oral edible gels were prepared using gelling agent low acetylated gellan gum and sodium citrate in different concentrations. The prepared edible gel formulations were evaluated for gelation time, appearance, texture, viscosity, pH, syneresis, drug-excipient compatibility studies by fourier transform infrared FTIR and percentage of drug release from the gel formulation.Results: Formulation containing gellan gum (0.4 % w/v) and sodium citrate (0.3 % w/v)) was found to be “spoon thick” in consistency that is accepted for dysphagic patients as per national dysphagia diet task force. This formulation showed more than 95 % drug release within 12 min and found to be stable for 6 mo. All other parameters tested were optimal. Hence, this formulation was considered optimized.Conclusion: From this study, it can be concluded that the novel edible gel dosage form containing Carvedilol can be formulated and this dosage form may prove to be more efficacious in the treatment of hypertension in dysphagic patients.
PHYTOCHEMICAL EVALUATION OF PUNICA GRANATUM L. LEAF EXTRACT
Authors: Sreedevi P. , Vijayalakshmi K. , Venkateswari R.
Published: September 2017

Objective: This study was conducted to assess the phytochemical constituents in Punica granatum L. Leaf extracts (PGLE) using standard methods.Methods: The leaf powder was extracted using solvents namely aqueous, hydroalcohol, ethanol, ethyl acetate and n-hexane. Qualitative and Quantitative phytochemical screenings of PGLE were assessed by standard methods.Results: All the leaf extracts were positive for a wide range of bio-active compounds except n-hexane. The result has showed that the maximum amount of total phenols (394.16 mg/g DW of extract), total tannins (210.5 mg/g DW of extract), flavanoids (147.4 mg/g DW of extract) and total triterpenoids (112 mg/g DW of extract) were noted in ethanolic extract of P. granatum leaf (EPGL). The biological assay revealed that relevant amount of carbohydrate, protein, lipid and alkaloid in EPGL.Conclusion: The findings of this study concluded that the EPGL had potential bioactive substances that may be used as pharmaceutical ingredients for formulation of new or prospective potent drug to cure wide range of metabolic diseases.
COMPARISON BETWEEN DEXAMETHASONE AND COOL JAW WRAP ON POSTOPERATIVE PAIN AFTER SURGICAL REMOVAL OF LOWER WISDOM TEETH
Authors: Al-fahad Nasma M. , Shallawe Wael Sheet
Published: September 2017

Objective: The aim is to compare between the effect of cool jaw wraps and dexamethasone injection on postoperative pain and evaluate the quality of life after surgical removal of lower wisdom tooth.Methods: Extraction of impacted lower third molar will surgically operate (by the same difficulty of surgical extraction and same operator) on 30 patients, which divide into three groups, each group have 10 patients.We will instruct the patients in cool jaw wrap group to put cool jaw wrap after the operation. While the second group give dexamethasone injection after the operation. The last group which is the control group will left them with the usual instruction postoperatively.This study evaluates the facial pain, swelling, and trismus on days 1,2 and 7 postoperatively. Objective measurements of swelling, pain, and trismus were undertaken at days 1, 2 and 7. The quality of life questionnaire was estimated at day 7 postoperatively.Results: Cool jaw wrap showed no significant differences on the postoperative pain when it used after surgical removal of the lower third molar BUT have significant differences on the Quality of life of patients.Conclusion: Cool jaw face wrap can be recommended as a safe method that participates in some degree to reduce postoperative pain, it easy to handle, comfort, avoiding damage by freezing due to the barrier between cool jaw and skin and the patient can avoid the side effect of dexamethasone and the phobia from the injection.
EFFECT OF SHILAJIT ON EXPERIMENTAL MODELS OF INFLAMMATORY BOWEL DISEASE IN RATS
Authors: S. Neelima , Babu T. Naresh , M. Pradeep Kumar
Published: September 2017

Objective: Inflammatory bowel disease (IBD) is a chronic condition of the intestine with unknown etiology involving multiple immunes, genetic and environmental factors. Oxidative stress is believed to be a key factor in the pathogenesis and perpetuation of the mucosal damage in IBD. The present study is to elucidate the effects of shilajit extract on the extent and severity of enterocolitis induced by subcutaneous administration of Indomethacin (7.5 mg/kg b. w) in Wistar rats.Methods: Study comprised of 6 groups (n=6), normal vehicle control, indomethacin-induced (7.5 mg/kg, 2days), shilajit alone 50 mg/kg b. w, shilajit treated groups (25 and 50 mg/kg, p. o) and sulfasalazine treated (100 mg/kg, p. o) groups. Drug treatment continued for 11 d and on 12th d scarification was done. The colonic mucosal injury was assessed by macroscopic scoring, biochemical (LDH, MPO, GSH and LPO) tests were performed.Results: Pretreatment with shilajit showed a decrease in macroscopic scores, LDH, MPO, LPO and elevation levels of GSH as compared to the indomethacin-treated group.Conclusion: The present study suggests that the protective effect of shilajit in indomethacin-induced enterocolitis might be attributed to its scavenging effect on oxygen-derived free radicals and may be beneficial in patients with inflammatory bowel disease.
IN SILICO STUDIES OF THE SECONDARY METABOLITES OF SOLANUM TORVUM SW. FOR THEIR ANTIASTHMATIC ACTIVITY
Authors: R. Soorya , P. Dhamodaran , R. Rajesh Kumar , B. Duraisamy
Published: September 2017

Objective: Solanum torvum Sw., Family: Solanaceae, commonly known as Turkey Berry is used by the traditional tribes for the treatment of cold, cough, tuberculosis, hepatotoxicity, cancer, etc. The action of the plant towards the treatment of these diseases has been proven except for asthma. The present study is to prove the antiasthmatic activity of methanolic extract and the secondary metabolites of Solanum torvum Sw using in silico docking studies in compare to reference standard Dexamethasone, a synthetic cortisone derivative.Methods: The GC-MS analysis of the dried methanolic extract of the dried fruits of Solanum torvum Sw. and the total saponin fraction has been carried out to know the important moieties that are responsible for the antiasthmatic activity.Results: The results from the docking studies showed that the compounds Cholesta-5,7,9-(11)-trien-3-ol,4,4-dimethyl, (3á); Lanosta-7,9-(11),20-triene-3α, 18-diol, diacetate and Cholestan-26-oic acid,3,7,12,24-tetrakis (acetyloxy), methyl ester, (3à,5á,7à,12à) were found to have significant scores of-6.8,-6.9 and-6.9 respectively towards Glucocorticoid receptor protein (Gr), (PDB id: 4UDC) which is very similar to the affinity of the standard (-7.1). These compounds passed the drug-likeness test. A modification in the structure can be brought, which makes the compounds more potent. The compounds 9, 12-Octadecadienoic acid, ethyl ester; Hexadecanoic acid, ethyl ester; 9-Octadecenoic acid (Z), methyl ester; Oxacycloheptadec-8-en-2-one, (8Z) have passed the Blood Brain Barrier (BBB) filter of the drug-likeness test.Conclusion: The antiasthmatic activity of the drug may be due to the similarity with the structure of Dexamethasone. Further research can be carried out in order to improve the clinical significance of these extracts and its metabolites.
BIOEQUIVALENCE STUDY OF ANTIDIABETIC ACTIVITY BETWEEN TWO MARKETED FORMULATIONS OF METFORMIN ON GLUCOCORTICOID-INDUCED HYPERGLYCEMIA IN RABBIT
Authors: Das Debanga , Chakraborty Jashabir , Dash Suvakanta
Published: September 2017

Objective: Bioequivalence studies are the commonly accepted methods displaying therapeutic equivalence between two products. This study was conducted to evaluate the bioequivalence study of anti-diabetic activity between two formulations of metformin tablets which were marketed in India.Methods: In in vitro study five essential in vitro tests including disintegration, weight variation, hardness, friability and a comparative in vitro dissolution study were performed.Results: For in vivo study adult male rabbits were divided into three groups of two each. The first group is regarded as control group received 3 ml of normal saline daily by using the gastric tube for 15 d and the second and third group received (0.35 mg/Kg B.W. single dosage) of dexamethasone tablets which were powdered, dissolved in 3 ml of normal saline daily for 15 d. After 15 d the blood glucose of second and third group was estimated and after that received formulation X and formulation Y, dissolved in 3 ml of normal saline daily for 15 d at the dose of 0.5 gm/kg body weight orally. After 15 d again blood glucose of second and third group was estimated and compare the results of both the group. This shows the favourable response of metformin against glucocorticoid-induced renal damage and hyperglycemia.Conclusion: Results of this study showed that the extent, rate of absorption and anti-diabetic activity of two different formulations of metformin tablets are bioequivalent to each other.
PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF IPOMOEA AQUATICA FORSK. (FAMILY-CONVOLVULACEAE)
Authors: Chaudhury Imdadul Haque , Das Trishna , Saha Dipankar
Published: September 2017

Objective: To evaluate the antimicrobial activity ethanolic extracts of stems of the plant Ipomoea aquatica Forsk. (family-Convolvulaceae) along with preliminary phytochemical analysis.Methods: The ethanolic extract of stems of the plant Ipomoea aquatica Forsk. (family-Convolvulaceae) were prepared and analyzed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungus using agar well diffusion method.Results: The present experiment shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of stems of the plant Ipomoea aquatic Forsk. (Family-Convolvulaceae). Various phytochemical analysis revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. All the extracts of the plant showed antimicrobial activity against most of the test organisms.Conclusion: The present study concluded that ethanolic extract of stems of the plant Ipomoea aquatica Forsk. Contain the high presence of phytochemical. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.
SIMULTANEOUS ESTIMATION OF THIOCOLCHICOSIDE AND ACECLOFENAC BY HPTLC
Authors: Syal Pratima , Kumar Ravinder , Arora Govind
Published: September 2017

Objective: Therefore the aim of the present work was to develop simple, precise and accurate HPTLC method for simultaneous determination of THIO and ACE in pharmaceutical dosage form and application of the method for dissolution study with help of HPTLC method.Methods: The TLC procedure was optimized in view to develop a simultaneous assay method for THIO and ACE. HPTLC Pre-coated plates silica gel 60 F254 20×10 cm, layer thickness 0.2 mm (Merck, Germany). The samples were spotted in the form of bands (8 mm) with a Camag 100 microliter sample (Hamilton) syringe on silica gel precoated aluminium 60F254 plates. The mobile phase consisted of methanol: chloroform: water (9.6: 0.2: 0.2 v/v/v) and 10 ml of mobile phase were used per chromatography run. Plates were scanned over the range of 200-400 nm and the spectra were overlain.Results: The detector response was found to be linear in the concentration range of 0.03-0.180 µg/band and 0.75-4.5 µg/band for THIO and ACE and noting the peak areas. Accuracy of the assay method was evaluated with the recovery of the standards from excipients. The mean percentage recoveries obtained for THIO and ACE were 99.34% and 99.08%, respectively, reported. The peak purity of both drugs was assessed by comparing the respective spectra of standard drugs and samples at peak start, peak apex and peak end positions of the spot.Conclusion: HPTLC, with its advantage of low operating cost, high sample thought and minimum sample preparation need is now days preferred as routine analytical techniques for control and assurance. The validated HPTLC method employed here proved to be simple, fast, accurate, precise and sensitive, thus can be used for routine analysis of Thiocolchicoside and Aceclofenac in tablet dosage form.
A COMPARATIVE EVALUATION OF ANTI-DIABETIC POTENTIALITY FOUND IN DIFFERENT MARKETED POLYHERBAL FORMULATION USING GLUCOCORTICOID-INDUCED HYPERGLYCAEMIA IN RABBIT
Authors: Boruah Pranjal , Chakraborty Jashabir , Dash Suvakanta
Published: September 2017

Objective: The aim of this study was performed to evaluate Antidiabetic potentiality found in different marketed polyherbal formulation using glucocorticoid-induced hyperglycaemia in the rabbit.Methods: The potentiality of different polyherbal formulation was investigated using dexamethasone (DEX) induced hyperglycaemia in Rabbit. Eight male rabbits were divided into four groups of two each. The first group is regarded as control group received 3 ml of normal saline daily by using the gastric tube for 15 d and remaining three group received (0.35 mg/Kg B.W. single dosage) of dexamethasone tablets which were powdered, dissolved in 3 ml of normal saline daily for 15 d. After 15 d the blood glucose estimated by using a glucometer and it is found that DXE treatment leads to significant increase in levels of glucose and a significant decrease in body weight. After that second group received metformin tablet. The third and fourth group received polyherbal formulation A and formulation B, which are powdered and dissolved in 3 ml of normal saline daily for 15 d at the dose of 0.5 gm/kg body weight orally. After completion of regular administration for 15 d, the blood glucose was again estimated and compare the results of each the group.Conclusion: The Anti-diabetic polyherbal marketed formulations were having less side effect as compared to standard metformin tablet (e. g. body weight loss). And both the polyherbal formulations were found a therapeutic equivalence to each other, also having the approximately similar potentiality to standard metformin tablet.Results: The result was found that the polyherbal marketed formulations were having less side effect as compared to standard metformin tablet (e. g. body weight loss). And both the polyherbal formulations were found significantly decreased in blood glucose level at equal potentiality, which can be consider as therapeutic equivalence to each other, and both the formulation also having the approximately similar potentiality to standard metformin tablet.
PRELIMINARY PHYTOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF FLACOURTIA JANGOMAS FRUITS
Authors: Das Sumit , Dewan Nuruzzaman , Das Kapil Joyti , Kalita Dhritishmita
Published: September 2017

Objective: To determine phytochemical, antimicrobial and in vitro antioxidant activity of Flacourtia jangomas (Family: Flacourtiaceae)Methods: The fruit extracts of the plant was taken for the phytochemical screening to identify the phytochemicals present in it. The extracts of the fruits were evaluated for antimicrobial activity against S. aureus and Escherichia coli by disc diffusion method. In vitro, antioxidant activity was performed by reducing power method, TBA method.Results: The n-butanol extracts showed significant antimicrobial activity against all the strains as compared to the standard drug. The extracts also showed antioxidant activity against the standard ascorbic acid.Conclusion: The extracts showed a high amount of antimicrobial activity and antioxidant activity.
PRELIMINARY PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT ACTIVITY OF ARAUCARIA COLUMNARIS BARK PEEL AND COSMOS SULPHUREUS FLOWERS
Authors: Jadav Krishma M. , Gowda K. N. Ninge
Published: September 2017

Objective: Four different extracts of Araucaria columnaris (bark peel) and Cosmos sulphureus (flowers) were screened for their phytochemical composition, and free radical scavenging activities.Methods: DPPH method was used to test the antioxidant activity for extracts.Results: Among the different extracts tested, the methanol extract of both the plant species showed significant radical scavenging activities. Phytochemical analysis of the extracts revealed that the radical scavenging activities might be due to the presence of flavonoids, tannins and phenolic compounds.Conclusion: The results obtained suggest that Araucaria columnaris (bark peel) and Cosmos sulphureus(flowers) could be exploited in the treatment of various diseases like cancer, cardiovascular diseases and infection diseases.
EVALUATION OF ANTI-HYPERLIPIDEMIC ACTIVITY OF CAPSICUM FRUTESCENS EXTRACT
Authors: Thomas Licto , Kamath Jagadish Vasudev
Published: September 2017

Objective: The objective of the study was to evaluate the in-vivo anti-hyperlipidemic activity of Capsicum frutescens extracts.Methods: The dried fruit powder were extracted with a three liquid phase extraction system. The acetone extract was isolated and the anti-hyperlipidemic activity was evaluated.Results: The anti-hyperlipidemic study was carried out by inducing hyperlipidemia in rats by means of triton. The serum collected was analyzed for total cholesterol, triglyceride, low-density lipoprotein and high-density lipoprotein.Conclusion: The result of the present study revealed that the acetone extract of the fruits of Capsicum frutescens possess anti-hyperlipidemic activity.
EFFECT OF BACOSIDE A ON LIPID PEROXIDATION IN D-GALACTOSE INDUCED AGING MICE
Authors: Pawar Sushama , Jadhav Manmohini
Published: September 2017

Objective: Bacoside A is a major bioactive constituent of Bacopa monnieri L having antioxidant property. The objective of this study was to evaluate the effect of Bacoside A, on lipid peroxidation in brain, heart and liver during induced aging.Methods: Male Swiss albino mice, Mus musculus was used for the present investigation. Four experimental groups were used as Group I-Normal adult, Group II-D-galactose induced, Group III-D-galactose induced plus Bacoside A treated and Group IV-Natural aging. The effect of Bacoside A was studied against lipid peroxidation during induced aging. The level of lipid peroxidation in the form of MDA formation was determined and measured in brain, heart and liver.Results: The statistical data obtained were analyzed using one way ANOVA, control vs other groups and results were expressed as mean±SE. In Bacoside A treated group the lipid peroxidation level in heart, brain and liver was significantly decreased (p<0.001) compared to control group. A significant increase (p<0.0001) in the level of lipid peroxidation was observed in D-galactose induced mice. In natural aging group highly significant increase (p<0.0001) in initial lipid peroxidation, ascorbate dependent lipid peroxidation and spontaneous lipid peroxidation was observed.Conclusion: The observations revealed that, lipid peroxidation was reversed in Bacoside A treated group which may be due to antioxidant property of Bacoside A. Thus Bacoside A is able to ameliorate the stress induced changes in lipid peroxidation during aging. The findings also provide a theoretical basis for the development of novel therapeutic formulations, such as antioxidant supplementation to boost antioxidant defenses in the body.
SYNTHESIS AND SCREENING OF BENZOFURAN FUSED C-2,4,6-SUBSTITUTED PYRIMIDINE DERIVATIVES AS A NEW ANTIBACTERIAL AND ANTIFUNGAL AGENT
Authors: Rangaswamy Javarappa , Naik Nagaraja
Published: September 2017

Objective: Benzofuran and their heterocyclic analogs represent an important class of molecules which have a wide range of pharmacological activities. Therefore, in this study synthesis and antimicrobial activity of benzofuran fused C-2,4,6-substituted pyrimidine derivatives was extensively discussed.Methods: Benzofuran fused C-2,4,6-substituted pyrimidine derivatives (4a-k) were built by cyclo condensation, Claisen-Schmidt condensation followed by cyclization via coupling of benzoyl fragments, which include benzofuran, a pyrimidine ring and C-6 substituted phenyl residue with various substituents, connected by linker-S-band. The structures of the synthesized compounds were confirmed by analytical and spectral techniques and evaluated their antimicrobial activity.Results: The results of antibacterial and antifungal activity against various microbes, most of the compounds have shown considerable antimicrobial activity, but compounds 4g and 4e exhibits superior activity compared to standards, this may be due to presence bromo and fluro electron withdrawing substituent on the benzoyl moiety and more lipophilic nature of pyrimidine ring.Conclusion: According to the activity studies, it is observed that the synthesis and antimicrobial activity of benzofuran fused C-2,4,6-substituted pyrimidine derivatives have been shown better antimicrobial activity. The obtained results suggest that these classes of compounds can be considered as new hits for further structural optimization to obtain better antimicrobial drug development program.
EVALUATION OF PHYSICO-CHEMICAL PARAMETERS OF DIFFERENT SHODHIT GUGGUL
Authors: Patel Mayuree A. , Acharya Sanjeev R , Macwan Carol P. , Patel Tejas B. , Suhagia B. N.
Published: September 2017

Objective: The present study was aimed to identify the physicochemical data of shodhit guggul. Guggul is a gum-resin exudate from the plant Commiphora weightii (Arn.) Bhandari, belonging to Burseraceae family. In Ayurveda, guggul is always purified. This purification is known as Shodhan. Shodhan is a process by which guggul is made non-toxic, effective, suitable and fit for therapeutic purposes.Methods: The seven different shodhan dravya were used to prepare shodhit guggul. They were evaluated by performing physicochemical parameters including five different extractive value; total ash, acid insoluble, water soluble and sulphated ash value; pH, and loss on drying.Results: Analytical results of raw guggul showed total ash, acid insoluble ash, water soluble ash and sulphated ash value to 5.36±0.04%, 0.96±0.03%, 4.51±0.03 % and 8.40±0.04% respectively. These all values of each shodhit guggul were different. The extractive value of raw guggul was comparable with standard value while the extractive value of each shodhit guggul was totally different. The pH value of 1% w/v and 10% w/v aqueous solution of raw guggul was 6.44±0.18 though pH of each shodhit guggul was changed. The loss on drying of raw guggul was found to be 1.88±0.02%w/v, however, this value was different for each shodhit guggul.Conclusion: The present study revealed that the different shodhan process with specific shodhan dravya affects the physicochemical parameters. The analysis and comparison of the data showed the difference in the properties of seven shodhit guggul with respect to raw Guggul.
NANOCRYSTAL SUSPENSION OF CEFIXIME TRIHYDRATE PREPARATION BY HIGH-PRESSURE HOMOGENIZATION FORMULATION DESIGN USING 23 FACTORIAL DESIGN
Authors: Bhaskar Rajveer , Patil Prakash Hiraman
Published: September 2017

Objective: In the present study, nanocrystal suspensions of cefixime trihydrate were prepared with the objective of providing increased solubility and stability with their nanoscopic size and thus developing the formulation of enhanced bioavailability potential.Methods: Nanocrystal suspensions were prepared by high-pressure homogenization technique using PVP K-30 as a stabilizer and evaluated for particle size, polydispersity index, zeta potential, permeation and drug release.Results: Particles of average size 143.5 nm having a polydispersity index of 0.269 were produced. Zeta potential was found to be −36.6 mV and the formulation was found stable on the basis of results obtained from differential scanning calorimetry and Fourier transform infrared spectroscopy studies. Optimized formulation showed 89.79 % and 88.38% drug lease and permeation respectively.Conclusion: The drug release and ex-vivo permeation studies revealed enhanced permeation of drug, as desired, indicating its potential for an attempt towards successful nano crystal formulation.
PREPARATION AND EVALUATION OF CLARITHROMYCIN LOADED BISMUTH SULFIDE (Bi2S3) NANOPARTICLES UTILIZING NANOTECHNOLOGY AS A NOVEL DRUG DELIVERY SYSTEM
Authors: Abdulbaqi Mustafa R.
Published: September 2017

Objective: This study was designed to improve the solubility and biological activity of class II drug clarithromycin (CLA) by utilizing the nanotechnology as a novel drug delivery system.Methods: Bismuth sulfide (Bi2S3) nanoparticles were synthesized using chemical co-precipitation technique, while the loading of clarithromycin (CLA) with bismuth sulfide (Bi2S3) nanoparticles was achieved using incorporation method. The loading process, as well as particle size reduction, were evaluated using x-ray diffraction (XRD), furrier transformed infrared (FTIR) and atomic force microscopy (AFM). In vitro release study was performed using USP paddle apparatus type II in phosphate buffer solution pH 7.4. Disc diffusion method was the technique used to test the antibacterial activity of CLA before and after loading process.Results: Loading of CLA with Bi2S3 nanoparticles was accomplished successfully accompanied with particle size reduction within nano range as measured by AFM. In vitro release study showed a significant* increase in solubility and dissolution profile of CLA after loading process, which was also proven using XRD that indicate transformation from crystalline into more soluble amorphous structure. Susceptibility test displayed significant* potentiation of antibacterial activity at all tested concentrations against gram+ve bacteria Staphylococcus aureus and Bacillus subtilis after loading of CLA with Bi2S3 nanoparticles, while gram –ve bacteria E. coli showed no response for CLA before and after loading process.Conclusion: The solubility, as well as the antibacterial activity of CLA, were improved significantly* after preparation of nanotechnology based drug delivery system through the utilization of metal nanoparticles, Bi2S3, as nanocarriers for CLA.
IMPACT OF PATIENT COUNSELLING ON KNOWLEDGE, ATTITUDE, AND PRACTICE OF HYPERTENSIVE PATIENTS IN A TERTIARY CARE HOSPITAL
Authors: Thomas Juna Ann , Snigdha Kakani Sai , Karanath Preethy Mathew , Swaroop Ann Mary
Published: September 2017

Objective: Hypertension is a leading cause of death and disability worldwide and proper assessment of knowledge, attitude, and practice (KAP) factors are helpful in its management. Our aim was to assess the knowledge, attitude, and practice among hypertensive patients and the impact of patient counselling on the same.Methods: A prospective study was conducted among hypertensive patients from November 2015 to April 2016. Suitably designed and validated KAP questionnaire was used to evaluate the baseline KAP score. Patient information leaflets were provided after counselling. After 3 mo patients were followed up and their KAP scores were reassessed and data were analysed.Results: Before the intervention, the mean score±SD of knowledge, attitude, and practice were 11.76(SD±4.58); 5.14(SD±2.13); 9.26(SD±1.66) which increased to 14.29(SD±2.95); 5.74(SD±1.39); 10.32(SD±1.33) respectively after the intervention.Conclusion: Our study suggests that pharmacist mediated counselling plays a vital role in improving patients’ knowledge, attitude, and practice towards the disease management
A SELECTIVE AND SENSITIVE METHOD DEVELOPMENT AND VALIDATION BY LC-MS/MS APPROACH FOR TRACE LEVEL QUANTIFICATION OF POTENTIAL GENOTOXIC IMPURITY OF BOC EPOXIDE IN ATAZANAVIR SULPHATE DRUG SUBSTANCE
Authors: Subbaiah Nelaturi , Reddy Gopireddy Venkata Subba
Published: September 2017

Objective: To develop and validate a selective, sensitive, rapid and accurate method using LC-MS/MS technique to achieve efficient separation between active pharmaceutical ingredient (Atazanavir sulphate) and genotoxic impurity (BOC epoxide).Methods: The quantification was carried out using the column puro sphere star RP 18 e (length 150 mm, internal diameter 4.6 mm, particle size 3.0 µm) with electrospray ionization in multiple reaction monitoring (MRM) detection mode. Eluent-A was 0.1% formic acid in water and eluent-B was 0.1% formic acid and 0.1% ammonium hydroxide solution (25%) in acetonitrile. The isocratic mode of elution was carried out for the elution of impurity with the shorter run time of 6 min. The flow rate was 1.0 ml/min and column oven temperature was maintained 25 °C.Results: The method was validated as per ICH guidelines and arrived the limit of detection and limit of quantification for the potential genotoxic impurity and found to be 0.2 ppm and 0.5 ppm. The developed method was found linear in the concentration range of 0.5 ppm to 6 ppm and accuracy results were within the range. Conclusion: The developed short span method found to be selective, sensitive, precise and accurate for the quantification of the BOC epoxide genotoxic impurity in atazanavir sulphate drug substance.
HOMOLOGY MODELLING AND MOLECULAR DOCKING STUDY OF ORGANOPHOSPHATES AND PYRETHROIDS IN TERMS OF POTENTIAL TOXICITY
Authors: Pandey Ravi S. , Tiwari Rishikesh K. , Singh Shikha , Ghosh Somenath , Rai Sunil K. , Shree Priya
Published: September 2017

Objective: Though the adverse effects of pesticides used in agriculture may not immediately be visible in the human population however its long term exposure may cause detrimental effects by biomagnifications and bioaccumulation. Nowadays bioinformatics serves as an in silico tool not only for homology alignment but also for prediction of quaternary structures of biochemicals. The present study was aimed to compare the potential toxicities of triazophos and chlorpyrifos (organophosphates; OPs) and cypermethrin and deltamethrin (pyrethroids) and their interactions with cytochrome P450 functioning. Methods: The authors performed the BLAST for homology alignment for cytochrome P450 of human and Zebra fish and further proceeded for docking analysis of all the pesticides with cytochrome P450. Results: It was noted that 99% of query cover with 32% of homology in the sequences of cytochrome P450 between human and Zebra fish. Upon docking, the pesticide deltamethrin showed the highest interaction with cytochrome P450 with highest binding energy and least dissociation constant for Deltamethrin which was found to be 8.233 [kcal/mol] and 922849.687 [pM].Conclusion: Our preliminary results thus encompass/indicate that the deltamethrin is not only having detrimental effect on enzyme kinetics in general but also such similar effects be apprehended for human also.
DEVELOPMENT, FORMULATION AND EVALUATION OF A BILAYER GASTRIC RETENTIVE FLOATING TABLETS OF RANITIDINE HCL AND CLARITHROMYCIN USING NATURAL POLYMERS
Authors: Rajeswari Saripilli , Kudamala Sravya , Murthy Kollapalli Venkata Ramana
Published: September 2017

Objective: Bilayer gastric retentive floating tablets (BGRFT) with ranitidine HCl and clarithromycin using natural gums have been developed to prolong the gastric residence time and increase drug bioavailability. Literature review revealed no published studies on the present study.Methods: Immediate release (IR) layer prepared by using different diluents and super disintegrants like sodium starch glycolate, crosscarmellose sodium and crospovidone. Controlled released (CR) layer prepared by using neem gum, damar gum and copal gum. Prepared tablets were evaluated for in vivo and in vitro buoyancy, in vitro dissolution studies and fourier transformation-infrared spectroscopy (FTIR). Drug release was evaluated with zero and first order for release kinetics, Higuchi, Hixson-Crowell erosion models for release mechanism.Results: Prepared IR layer followed first order rate kinetics and CR layer followed zero order rate kinetics with non-Fickian diffusion mechanism. BGRFT also showed similar results as that of the individual layer. Optimized formulations were characterized by FTIR studies and found no interactions between drug and polymer.Conclusion: The results demonstrate the feasibility of the model in the development of BGRFT. BGRFT enhanced the drug release and finally the bioavailability of clarithromycin when compared with commercial tablet (Biomycin 250). The present study could establish the suitability of neem gum as CR polymer in the design of BGRFT.