bluuebunny/crossref_metadata_2025 huggingface dataset:
Ethno Veterinary Practices Followed by Attappadi Tribal Farmers of Kerala
Authors: Nisha A. , Rajkumar N. Vimal , Mansingh J. Paul
Published: October 2024

Background: The study was undertaken to document the different plants of ethno veterinary uses and to identify the most prevailing livestock disease category among livestock farmers of Attappadi using consensus methodology. Methods: This study was conducted in Attappadi with a structured interview with tribal farmers, Ethno Veterinary Medicine (EVM) healers and key informants engaged in the livestock farming. Result: There was great agreement among the informants regarding ethno veterinary uses of plants with Factor of Informants Consensus (FIC) value ranging from 0.50 to 0.97, with an average value of 0.87, used to generate novel, cost effective, active and eco-friendly herbal formulations for healthcare management. A total of 55 plant species under 39 families in Attappadi area have been documented for treatment of 15 categories of ailments.
Gabon - Mallette pour la présentation d’échantillons de bois
Authors: Rédacteur de la revue Bois et Forêts des Tropiques
Published: October 2024

Pas de résumé
HEPATITIS B VIRUS INFECTION AND ASSOCIATED FACTORS AMONG BLOOD DONORS IN ETHIOPIA: A CROSS-SECTIONAL STUDY
Authors: Sheik Abdifetah Abdulahi , Getnet Fental , Aden Mowlid Akil , Yusuf Abdulahi Mohamud , Dhicis Ahmeddahir Abdi
Published: October 2024

Background: Blood transfusion is a mandatory therapeutic maneuver that provides life-saving benefits to patients who are suffering from severe anemia due to medical disorders. Purpose: this study was aimed to determine HBV infection and associated factors among blood donars in Jigjiga zone Somali region. Methods: A facility-based cross-sectional study design was conducted among a sample blood donor in Jigjiga city Blood Bank Somali regional state of Ethiopia. A standardized questionnaire using Epidata was used. A bivariate and multivariate logistic regression was used to assess the independent predictors association (pvalue<0.25) in bivariate as candidate of multivariable logistic regression model-A significant (pvalue<0.05) was considered as statistical significance. Results: A total of 323 blood donars participated with a response rate of 95.5%. The overall prevalence of hepatitis B virus infection was (7.1%) (95% CI: 4.3-10.2)After adjusted analysis, marital status being single (AOR=5.3 CI: 1.018-27.859), unsafe therapeutic drug injections (AOR=10.1 CI: 2.791-36.566) unprotected sex (AOR4.6=CI: 1.533-14.116), razor and sharp material sharing (AOR=5.5 CI: 0.852-16.530), having poor knowledge on hepatitis B virus infection (AOR=3.4 CI: 1.107-10.485) were identified as statistically significant associated with hepatitis virus infection. Conclusion: Overall prevalence of the study was 7.1%, which needs intervention by the government and other stakeholders.
UNDERSTANDING PUBLIC COMPLIANCE WITH COVID-19 HEALTH PROTOCOLS IN INDONESIA: A CROSS-SECTIONAL STUDY
Authors: Oktavia Nada Karisma , Boerman Baequni , Nasir Narila Mutia , Samara Shafira Salsabila
Published: October 2024

Background: The Coronavirus Disease 2019 has become a global pandemic, affecting many countries, including Indonesia. The number of COVID-19 cases in Indonesia, particularly in Jakarta, continues to increase. Purpose: This study aimed to identify the factors influencing compliance with the COVID-19 prevention protocol among Jakarta's residents. Methods: An accidental sampling technique was used in this study, with the calculation of hypothesis testing of the difference between two proportions, resulting in a total sample of 440 respondents. Data was collected through an online survey using Google Forms. Results: The study found that education level (p-value=0.003; OR=1.818), knowledge (p-value=0.01; OR=6.669), attitude (p-value=0.01; OR=7.789), perception of vulnerability to COVID-19 (p-value=0.01; OR=3.307), social support (p-value=0.01; OR=3.733), and supervision (p-value=0.01; OR=3.435) had a significant relationship with compliance with the COVID-19 prevention protocol. However, facilities and infrastructure (p-value=0.341; OR=0.453) had no significant relationship with compliance. From the odds ratio data, knowledge and attitude significantly influence adherence to COVID-19 preventive measures. Individuals well-informed about COVID-19 are 6.6 times more likely to comply, and a positive attitude increases this likelihood by 7.8-fold. This underscores the strong link between awareness and adherence to recommended protocols. Conclusion: This study highlights the importance of understanding the factors affecting adherence to COVID-19 prevention protocols. It guides policymakers and public health professionals in devising effective strategies to curb the spread of the disease.
Method оf Load Balancing іn Distributed Three-Layer IoТ Architecture
Authors: Haidai Anatolii , Klymenko Iryna
Published: October 2024

Due to the growing number of IoT devices and the need for fast data processing with minimal delays, traditional cloud computing is becoming less efficient. To solve this problem, the concept of edge computing is used, which, although it increases performance, complicates system management and requires effective load distribution to ensure a balance between the use of edge nodes and the speed of computation. The purpose of this work was to develop a method of load balancing in the three-layer architecture of the IoT system, taking into account the actual loading of nodes.
A review of the literature was conducted and an architectural concept was chosen that corresponds to new trends and consists of three layers: devices that generate data, edge nodes that process information, and the cloud that stores data and provides it to users. A system prototype was created, which includes several Edge nodes based on the Ubuntu Server 24.04 operating system and data servers based on Raspberry Pi Desktop. A mathematical model has been developed that allows you to estimate the load on nodes depending on the type of tasks performed. On the created prototype, the efficiency of the method was checked using a mathematical model.
The research results showed that the developed method successfully distributes the load between Edge-nodes with the help of special scripts and elements of the monitoring system, which is reflected in the server load graph. The proposed method can improve system performance due to automatic load distribution among nodes. This approach can become part of a more comprehensive strategy to improve the performance and reliability of IoT systems using edge computing. Using components of the monitoring system for different platforms with different power allows to reduce the cost of the system by using cheaper and less powerful computing devices.
Impact of the grazing ban on the forest soil nutrient dynamics in the Sikkim Himalaya, India
Authors: Sharma Ghanashyam , Pradhan Bharat K. , Chhetri Prakash
Published: October 2024

With more than two decades of livestock grazing prohibition in Sikkim, understanding its impact on forest ecosystem, particularly soil nutrient dynamics, is crucial. Soil nutrient analyses were conducted on samples from four study sites at two depths (0–15 cm and 15–30 cm) during rainy and winter seasons. The treatments were (1) current grazing forests (CGFs; areas where herders graze their animals), (2) old grazed forests (OGFs; grazing was prevalent before the ban on grazing), (3) ungrazed forests (UGFs; not impacted by livestock grazing), and (4) abandoned Kharkas (AKs; open-grazing pastures). Mean soil total nitrogen content in CGF (0.45%) > OGF (0.41%) > UGF (0.37%) > AK (0.34%) showed significant differences (p < 0.05). Similarly, the mean available phosphorus content in CGF (0.12%) and OGF (0.009%) was significantly (p < 0.05) higher than UGF (0.007%) and AK (0.006%). Likewise, the mean soil total phosphorus in CGF (108.21 mg 100 gm–1) > OGF (94.75 mg 100 gm–1) > UGF (89.02 mg 100 gm–1) > AK (83.24 mg 100 gm–1) revealed significant (p < 0.05) variation. Mean soil organic carbon and soil organic matter in CGF (3.86%, 6.64%) > OGF (3.76%, 6.47%) > UGF (3.56%, 6.13%) > AK (3.34%, 5.75%) and carbon:nitrogen ratio in CGF (8.61) < OGF (9.19) < UGF (9.53) < AK (9.97) showed significant differences (p < 0.05). Our results confirm that the management of controlled, regulated, and rotational grazing is beneficial for improving the forest ecosystem health in terms of soil nutrient availability.
Screening for compulsive exercise: development of cutoff scores to identify individuals at risk
Authors: Cosh Suzanne M. , McNeil Dominic G. , Tully Phillip J.
Published: October 2024

Compulsive exercise (CE) is proposed to be a unique disorder within the feeding and eating spectrum, with the potential to cause a range of physical and mental health outcomes. However, it often remains undetected, highlighting the need to enhance screening for CE, especially outside of eating disorder (ED) treatment settings. Although CE is commonly assessed using the Compulsive Exercise Test (CET), there are currently no established cutoff scores for identifying CE in community settings or outside of ED populations. This study utilized two samples—a general exercise population and a community sample recruited to capture individuals with elevated mental health symptoms—to identify a suitable cutoff for the CET. Receiver operating characteristic analyses were used, and CE cases were classified in three ways. The area under the curve was moderate to high for detecting CE, and optimal cutoff scores were consistent across CE markers and samples. The present study suggests that a cutoff score of 13.5, which increases sensitivity and balances specificity, can be used to detect possible cases of CE in the community. These results have practical implications for screening across diverse settings and can contribute to further research and understanding of CE.
Analysis of The Implementation of Human Resource Development at The Lembaga National Single Window
Authors: Azhari Juwita Fatin , Widya Parimita  , Rizki Firdausi Rachmadania
Published: October 2024

The purpose of this research is to find out the implementation of human resource development through education and training methods and to be able to find out the impact of the implementation of human resource development for companies. This study uses a qualitative descriptive approach by describing and analyzing problems related to human resource development. Primary and secondary data are the basis for qualitative research. The primary data of this study was obtained through observations, interviews, and questionnaires, while secondary data was obtained from books, previous research, and related scientific articles. From the results of this study, it can be seen that the Lembaga National Single Window has implemented a human resource development program through education and training methods. However, from the program for education and training planning, it is carried out at the same time as planning budget needs and adjusted to the needs of the organization, not just in organizing education and training programs. Thus, this study shows that human resource development can be beneficial for companies, such as motivating employees to participate in training held by external parties to increase employee value, companies are expected to discuss more intensively with all units and involve leaders to better synchronize organizational needs with individual development needs and appropriate types of education and training.
Analysis of the Use of E-resources Applications at the Faculty of Economics, Jakarta State University
Authors: Yuliana  , Roni Faslah  , Suherdi
Published: October 2024

This research was conducted at the Jakarta State University library for students of the Faculty of Economics to understand the utilization of e-resources in assisting their learning activities. The study employed a qualitative research design to gain deep insights into social and human phenomena, as suggested by Walidin, Saifullah, and Tabrani (2015). Data were collected over a three-month period using interviews, observations, and documentation reviews. The primary data sources included descriptive data from observable individuals and actors, interviews conducted to exchange ideas and information with respondents, systematic observations of user behavior at the library, and documentation reviews of past events and supporting data from the interviews and observations. The analysis used descriptive methods to systematically and accurately describe the current conditions regarding the use of e-resources. This research aims to provide an in-depth description of the utilization of e-resources and the challenges faced by students. The findings suggest several approaches to encourage the use of e-resources, including active promotion on social media and digital platforms, collaboration with lecturers, and provision of support services or technical assistance.
The The Influence of Perceived Waiting Time, AI Utilization, and Medication Information Delivery on Word of Mouth: A Qualitative Preliminary Study at Hospital Balikpapan
Authors: Rozet Stefly J. A. , Yusriani Sri , Sitompul Rustina , Supriyanto Edi , Jasmine Khanza , Utomo Kabul Wahyu
Published: October 2024

Abstract:
There is currently a lack of comprehensive, intuitive, and usable formative evaluation frameworks to improve word of mouth (WOM) at our hospital. Therefore, we aimed to develop and apply such a framework through a literature review analysis. This preliminary study seeks to analyze the influence of perceived waiting time, AI utilization, and medication information delivery on WOM at Hospital Balikpapan through a qualitative approach. Using a literature review method, we examined 20 relevant studies to understand the relationships between waiting time, AI utilization, medication information, patient satisfaction, and WOM. The findings suggest that patient satisfaction plays a crucial role in mediating the effects of waiting time, AI utilization, and medication information on WOM. Additionally, AI-based tools can help reduce waiting times by optimizing scheduling and resource allocation, and improve medication information delivery through AI-led chatbot services, providing accurate and timely drug information to patients. This study highlights the importance of addressing patient expectations and improving communication in pharmaceutical services to enhance patient satisfaction and positive WOM. Furthermore, the acceptance and utilization of AI in healthcare can significantly contribute to better patient experiences. Our review underscores the need for continuous improvement in service delivery and the potential benefits of integrating AI technologies in hospital settings. By focusing on these areas, hospitals can enhance patient satisfaction, leading to increased positive WOM and overall better healthcare outcomes.
 
Keywords: Perceived waiting time, AI utilization, medication information delivery, patient satisfaction, word of mouth, qualitative study, hospital.
The Influence of Learning Discipline and Learning Facilities on Learning Achievement with Parental Support as a Moderating Variable for Vocational School Students Majoring in Accounting in East Jakarta
Authors: Wulandari Nadya indah , Sumiati Ati , Kismayanti Respati Dwi
Published: October 2024

The aim of this research is to find out whether learning discipline influences learning achievement, learning facilities influence learning achievement, parental support moderates the influence of learning discipline on learning achievement, and parental support moderates the influence of learning facilities on learning achievement among vocational school students majoring in accounting in Jakarta East. The research was carried out in SMKN 10, SMKN 40, and SMKN 48. This research uses quantitative research with survey methods.The population came from State Vocational School students majoring in accounting in East Jakarta. The sampling technique used in this research was simple random sampling. The total sample was 168 respondents. Data collection technique used was a questionnaire processed with SPSS and documentation. The results show that learning discipline has an effect on learning achievement, learning facilities have no effect on learning achievement, learning discipline moderated by parental support has an influence on learning achievement, learning facilities moderated by parental support has no effect on learning achievement. Recommendations for further research are:can explore other factors that influence learning achievement, expand the area of ​​data collectionso that new things can be discovered that can influence performance Study.
Effect of Third-Party Funds on Profitability with Credit Distribution as Moderating Variable
Authors: Ida Ayu Gede Utami Dewi
Published: October 2024

The economic stability experienced by the banking sector was caused by the Covid-19 pandemic, one of which is in conventional commercial banks. This is inseparable from the sentiment of investors who think that the government has not seriously handled Covid-19, resulting in investors preferring to withdraw their funds rather than depositing their funds in banks which then impact on decreasing bank profitability. The research objectives to be achieved in the form of empirical evidence include the effect of third party funds on profitability with lending as a moderating variable. The study focused on conventional banks listed on the Indonesia Stock Exchange during the period of 2019-2022. Purposive sampling method was used in determining the research sample namely 24 companies with 96 observations. The collection used non-participant observation method by accessing the Indonesia Stock Exchange website. Signaling theory is used to support the results of this study. Data analysis was carried out with Moderated Regression Analysis (MRA) and using the SPSS application. The results of the research show that third party funds have a positive and significant effect on profitability and credit distribution was able to strengthen the effect of third party funds on profitability. The implication of this research is that it is able to support and add knowledge related to the application of signal theory and provide benefits to interested parties, especially companies and investors related to third party funds and credit distribution that can increase profitability.
Analysis of Effectiveness and Efficiency to Assess the Financial Performance of the District/City Government of West Java Province for the 2020-2022 Period
Authors: Rafliaulia Mohamad , Zakaria Adam , Khairunnisa Hera
Published: October 2024

This study aims to analyze financial performance using the effectiveness ratio and efficiency ratio in districts/cities in West Java Province for the period 2020-2022. The method used in this study is quantitative descriptive with secondary data collection methods from all financial data in the Budget Realization Report (LRA) and Regional Financial Information System (SIKD) in districts / cities in West Java Province in the 2020-2022 period . Data analysis techniques are carried out by searching for research data, calculating ratios, measuring ratios, analyzing the results of data calculations, and continuing with interpretation of district/city financial performance data in West Java Province in 2020-2022.
The results of this study indicate that the financial performance of the effectiveness ratio of districts or cities in West Java in the 2020-2022 period has decreased on average, but is still at an effective level with a percentage of 99.8%. Meanwhile, the financial performance of the efficiency ratio of districts or cities in West Java in the 2020-2022 period remained stable or constant on average, but was still at a fairly efficient level with a percentage of 91.5%. In the effectiveness ratio of districts or cities in West Java in 2020-2022, the highest score was in Karawang Regency (2020) and the lowest in Kuningan Regency (2022). And in the efficiency ratio of districts or cities in West Java in 2020-2022, the highest score is in Pangandaran Regency (2022) and the lowest is in Pangandaran Regency (2020). Therefore, it can be concluded that not all regencies or cities in West Java Province have been effective and efficient in the 2020-2022 period, so local governments must pay more attention to returning so that their regions can develop and get better. Recommendations for future researchers are that researchers are expected to expand their research period, to see how the development of financial performance in their respective regions.
The Analysis of Factors That Can Affect Customer Retention of E-Commerce Users
Authors: Nur Firdausi Aprelia
Published: October 2024

This study aims to analyze factors that can affect customer retention of e-commerce users, in this study, the scope of research objects set by researchers in accordance with the problems to be studied is about the factors that affect customer retention of application users to use the application. This type of research is quantitative, the population in this study is the use of the Shopee application in Indonesia, the number of samples used in this study is 300 respondents with criteria, Active users of Shopee online applications / websites in Indonesia, Users who are over 17 years old and have made purchases at least 3 times. The data analysis technique used in this study is Structural Equation Modeling (SEM) based on Partial Least Square (PLS) which is processed with the help of Amos 26 software. Structural Equation Modeling (SEM) is a data analysis technique that tests the integration of factor analysis, structural models, and path analysis for a causal relationship.
Analysis of Organizational Culture at PT Kereta Commuter Indonesia
Authors: Anisa Nabila Putri  , Susan Febriantina  , Adnan Kasofi
Published: October 2024

This research aims to analyze organizational culture and organizational culture obstacles that occur at PT Kereta Commuter Indonesia. PT Kereta Commuter Indonesia is a company that operates in the public transportation sector, especially commuter train services in the Jabodetabek area. Organizational culture is an important aspect that influences company performance, employee satisfaction, and the quality of service provided to customers. The method used in this research is a qualitative study in the form of interviews, observation, documentation and literature study methods.
Analysis of Service Excellent at PT Home Tester Indonesia
Authors: Amanda Julia Zahra
Published: October 2024

Service Excellent is an activity carried out by a service provider with the specific aim of providing satisfaction that can be felt by customers. Services must be connected to consumers' hopes and desires for the products or services offered by a company so that consumers feel satisfaction This Research is executed to find out the applied service excellent that is used by PT. Home Tester Indonesia. The  methode  of  research  used  is  qualitative descriptive that gives the portrayal of service excellent of PT. Home Tester Indonesia. Data  collection  techniques  arecarried  out  through, namely observation, interviews, and documentation. the result of this research shows the excellent service that is done by PT. Home Tester Indonesia is good based on the attitudes, abilities, attention, actions, and responsibilities performed by the employees of the PT. Home Tester Indonesia. However, those are considered not optimal as the employees performed delayed response in serving the community.
Analysis of the incoming and outgoing letter system in the Protocol Division at the Ministry of Agriculture.
Authors: Fatma Suci Ramadhani  , Susan Febriantina  , Rizki Firdausi Rachmadania
Published: October 2024

This research was conducted at the Ministry of Agriculture to find out how the incoming and outgoing letter management system was carried out by employees of the Ministry of Agriculture, especially in the field of protocols in taking care of work visits. What is the procedure used in attaching a working visit to the area to be visited to be seen by the development of agriculture, animal husbandry, and plantations in Indonesia. This research data was obtained through several methods, namely observation, interviews, and documentation. Analysis of this study was compiled using descriptive qualitative analysis methods to provide a comprehensive picture of the incoming and outgoing letter system. Descriptive qualitative methods are used to describe existence systematically, factually, and accurately. Provisions regarding the facts investigated. Therefore, this research is expected to provide in-depth insights about the procedure in making outgoing letters and receiving incoming letters. and how to store letters efficiently.
Analysis of Employee Work Professionalism at the Satpol PP Bekasi City Office
Authors: Nur Laela Triana , Puji Wahono  , Terrylina Arvinta Monoarfa
Published: October 2024

Professionalism in the Workplace is a very important value within an institution or organization, as it significantly affects employee performance. Professionalism is defined as a behavior that shows commitment and accountability as a professional, in terms of skills, abilities, and work ethics. In an institution, it is important to increase education and training for employees to improve their professionalism. Additionally, the institution should ensure a conducive work environment and provide incentives for employees who have high professionalism. This professionalism of employees can be one of the factors that distinguish a successful institution from an unsuccessful one.
The research aims to gain an understanding of professionalism at the Satpol PP Office in Bekasi City using a qualitative descriptive method. The research uses primary and secondary data, including observations, interviews, documentation, and supporting previous studies on the topic of work professionalism.
The results of the research show that employees’ perceptions of work professionalism are characterized by their commitment to their work, being on time, discipline, and not procrastination. Factors that contribute to employees working responsibly at the Satpol PP Office in Bekasi City include finding a balance between work and personal life, which can reduce stress and increase employee motivation. The office has taken steps to improve professionalism among employees by providing guidance and training, as well as imposing sanctions on those who do not work professionally.
Analysis of the Implementation of Digitization at the National Archives of the Republic of Indonesia
Authors: Astuty Rihanna  , Prof. Dr. Henry Eryanto, M.M.  , Dr. Terrylina Arvinta Monoarfa, SE., MM.
Published: October 2024

The aim of this research is to examine the obstacles that influence the archive digitization process at the National Archives of the Republic of Indonesia. Researchers collect data by visiting the research location directly. Research data was obtained through literature studies such as journals, books, direct observation by going into the field, and interviews with informants related to the topic studied. The research method used is descriptive analysis which aims to explain or describe a situation or phenomenon that occurs. This method allows researchers to export aspects more widely. The researcher selected 4 (four) informants who met the criteria based on the research objectives so that the researcher obtained clear and accurate information in the form of statements, information or data that could help in understanding the problem. It is hoped that the results of this research will add insight into the implementation of archive digitization.
The Effectiveness of Live Streaming Shopping Attributes on Shopee Live in Jakarta
Authors: Anastasya Yohana
Published: October 2024

Business actors must understand what attributes they need to focus on to increase live streaming shopping views and sales to run successfully. This study aims to determine the level of effectiveness of Live Streaming Shopping Attributes on Shopee Live in DKI Jakarta with a case study on fashion products. This research is a type of quantitative research with a survey method through filling out a Google Form questionnaire. The sample in this study used a non-probability sampling method with purposive sampling technique. The data analysis techniques used in this study are simple tabulation analysis, descriptive analysis, and average score. Based on the results of data analysis, the Live Streaming Shopping Attributes are categorized as "Very Good" and "Very Effective", supportted by Price and Promotion Attributes and Seller Presentation, Seller Interactivity, and Seller Guidance Attributes. After conducting research on the effectiveness of Live Streaming Shopping Attributes (LSSA) on Shopee Live in Jakarta, it is concluded that the effectiveness of LSSA is effective in making viewers live streaming shopping on Shopee Live watch to make purchase transactions on fashion products.
Analysis of COSO-Based Internal Control in the Management of School Operational Assistance Funds X
Authors: Hastari Rachmadani  , Pahala Indra , Khairunnisa Hera , Michael Musyaffi Ayatulloh
Published: October 2024

This study aims to analyze the implementation of internal control in the management of BOS X funds based on the five components of COSO internal control. As well as providing recommendations for corrective actions for control gaps. This research takes school X as the object of research. This type of research is qualitative with a descriptive analysis approach. The data sources used are primary and secondary data with interview and documentation methods. The results of this study show that internal control in the management of BOS funds has been running in accordance with the technical guidelines for the management of BOS funds. Although there are some internal control principles that are not in accordance with the COSO components.
Analysis of GoPay Application User Satisfaction Levels in DKI Jakarta
Authors: Ananda Dwi Andini
Published: October 2024

This study aims to analyse the level of user satisfaction of GoPay application users in DKI Jakarta. The research was conducted from September 2023 to July 2024, using a descriptive quantitative approach through a cross-sectional survey design. Data was collected using online questionnaires distributed via Google Forms to active GoPay users in DKI Jakarta, chosen due to the region's high e-wallet usage. The population consists of active GoPay users in DKI Jakarta, with the sample selected through non-probability sampling to represent this population. Data analysis was performed using SPSS 27, with statistical tests including standard deviation and t-tests conducted to evaluate the variability and significance of the responses. The results revealed significant variability in user satisfaction and reuse intention, with the highest standard deviation observed in the usefulness aspect. The findings indicate a diverse range of responses, suggesting varied user experiences and perceptions. The t-test results showed no significant differences in user satisfaction and intention to use, highlighting key factors influencing these metrics. The significance of these results lies in their potential application for improving e-wallet services. By understanding the diverse user experiences and identifying critical factors that affect satisfaction and reuse intentions, service providers can enhance their offerings to better meet user needs. This research contributes to the broader understanding of e-wallet user satisfaction, providing insights for future studies and practical implications for the fintech industry.
Analysis Of The Implementation Of Marketing Mix On Customer Satisfaction Of Shopee
Authors: Hidayat Febiana Rainy , Sholikhah Sholikhah , Agustin Pratama Sari Dewi
Published: October 2024

This research aims to find out how the marketing mix is ​​applied to consumer satisfaction on the Shopee platform in DKI Jakarta. The sample used in this research was 117 respondents with the criteria of being at least 15 years old, residing in DKI Jakarta and using the Shopee platform. This research uses four dimensions, namely product, price, promotion and place. This research uses descriptive analysis methods, Customer Satisfaction Index, Importance Performance Analysis and Gap Analysis. The results of the research show that implementing the marketing mix can provide satisfaction to Shopee platform consumers in DKI Jakarta. Based on the Importance Performance Analysis, there are three things that need to be improved, that is price with product quality, promotional safety so that there is no manipulative behavior and accuracy in product delivery. Based on Gap Analysis, it shows that the product and promotion dimensions need to be improved.
Analisis Perbandingan Antara Sistem Administrasi Perkantoran Konvensional dan Digital Pada Kantor Dinas Kependudukan dan Pencatatan Sipil Kabupaten Bangka
Authors: Putri Fathiya Annisa , Febriantina Susan , Firdausi Rachmadania Rizki , Firdausi Rachmadania Rizki
Published: October 2024

Along with the advancement of information technology, significant changes have occurred in the way information management and administrative processes are conducted. Traditional office administration has migrated to the digital world, resulting in a shift from conventional office administration based on the use of paper, physical documents, and manual processes to digital office administration that prioritizes the use of technology to store and manage various data and information. The digitization of office administration is flexible or adjusted to the development of a company, including government agencies. Government agencies are organizations with governance systems that refer to legislation. The Department of Population and Civil Registration of Bangka Regency is an example of a government agency with a function in the population system activities in Indonesia, including the management of identity documents. The high intensity of activities in issuing many identity documents, along with the growth in population, certainly requires the management of a digital administrative system.
Keywords: Comparative Analysis, Office Administration, Digitalization
Monoclonal Antibodies Capable of Inhibiting the Interaction of the Receptor Binding Domain of SARS-CoV-2 Virus with the Angiotensin-Converting Receptor 2 of Human Cells
Authors: Ivashchenko T. A. , Romanenko Ya. O. , Kartseva A. S. , Silkina M. V. , Mar’in M. A. , Khlyntseva A. E. , Zeninskaya N. A. , Shemyakin I. G. , Firstova V. V.
Published: October 2024

The aim of the work was to evaluate the ability of monoclonal antibodies to inhibit the interaction of the receptor binding domain (RBD) in S protein of SARS-CoV-2 virus variants, Wuhan-Hu-1 and BQ 1.1, with the angiotensin-converting receptor 2 (ACE2). Materials and methods. In this study, recombinant RBDs of Wuhan-Hu-1 and BQ 1.1 variants were used as antigens. For mouse monoclonal antibody (mMCA) production, hybridomas were cultured in vivo in BALB/c mice. mMCAs were isolated from ascitic fluid by ammonium sulfate treatment followed by purification through column affinity chromatography with Protein G Sepharose sorbent. The specific activity of mMCAs was assessed by immunoblot with recombinant RBD of Wuhan-Hu-1 variant. To identify the most promising mMCA, the neutralizing activity of mMCA was evaluated by enzyme-linked immunosorbent assay (ELISA) via immobilizing RBD on the surface of a microplate and using ACE2 in the form of horseradish peroxidase conjugate. Recombinant antigens were produced in ExpiCHO-S cell line (Gibco, USA). Results and discussion. Three mMCAs have been described as a result of the study: 5C3, 3F11, 1E6. All antibodies belong to immunoglobulins of subclass G and specifically interact with the RBD in S protein of SARS-CoV-2 virus. The most effective inhibition of the interaction between ACE2 and the RBD of BQ 1.1 strain was observed for murine MCA 3F11 (65 %), while the interaction with the RBD of Wuhan-Hu-1 strain was inhibited by mMCA 5C3 (91 %). The identified characteristics allow for considering the antibodies as potential candidates for the development of antibody-based therapeutics, thus expanding the possibilities of therapy for SARS-CoV-2 virus infection.
Analysis of the Genetic Determinants of Yersiniachelin Siderophore of Yersinia
Authors: Kuznetsova D. A. , Vodop’yanov A. S. , Trukhachev A. L. , Rykova V. A. , Podladchikova O. N.
Published: October 2024

The study is focused on the genetic determinants of the yersiniachelin (Ych) siderophore encoded by the ysu gene cluster and found only in Yersinia pestis and Yersinia pseudotuberculosis. The aim of the investigation was to analyze the genes encoding the biosynthesis of Ych and to assess the possibility of their use for intraspecies differentiation of Yersinia. Materials and methods. The study used complete nucleotide sequences of 583 Y. pestis strains and 300 Y. pseudotuberculosis strains from the NCBI database, as well as 38 Y. pestis and 88 Y. pseudotuberculosis strains from culture collection at the Rostov-on-Don Anti-Plague Institute of the Rospotrebnadzor. The genomes were analyzed using bioinformatics methods and PCR in silico and in vitro. Results and discussion. A comparison of the ysu cluster genes in two Yersinia species has revealed their high conservatism in the plague microbe, while differences in the structure of the transcription terminator of the Ych biosynthesis genes have been found in the pseudotuberculosis microbe. It turned out that the terminator contains nucleotide tandem repeats that differ in the number in different groups of Y. pseudotuberculosis strains, thus indicating their potential use for intraspecies differentiation of these bacteria. To assess the effect of the transcription terminator structure on the Ych expression by plague and pseudotuberculosis microbes, two recombinant plasmids containing PCR copies of Ych biosynthesis operons from Y. pestis KIM and Y. pseudotuberculosis YPIII have been constructed. The introduction of both plasmids into the Escherichia coli H1884 strain, devoid of its own siderophores, has demonstrated that the operon of the YPIII strain determines a significantly higher expression of Ych than that of the KIM strain. The results of the study show that the structure of the transcription terminator of the Ych biosynthesis operon has a considerable impact on the expression of this siderophore and can be used for intraspecies differentiation of Y. pseudotuberculosis strains during epidemiological monitoring.
Accidents in Laboratories Working with Pathogenic Biological Agents
Authors: Krepostnova I. M. , Gordeeva M. V.
Published: October 2024

Accidents during the work with infectious disease agents are one of the leading biological risk factors for employees, the public and the environment. Specialists in the field of biological safety should monitor and analyze information on these accidents in order to use the findings and implications to form a sustainable biosafety system both, at the facility and at the federal levels. To obtain the necessary information to analyze the causes and conditions of accidents, we have carried out a search of open data on the Internet. It has been found that the relevant information is more extensively and accessibly presented in foreign sources. Information on accidents that occurred abroad between 2010 and 2023 has been studied. Accidents associated with skin penetration and lesions were most often the obvious cause of laboratory acquired infection. Reports of similar accidents caused by injection needle sticks predominated. Accidents associated with breach of personal protective equipment (torn gloves) were a potential cause of infection when there was a concurrent violation of the skin integrity or in the case of skin lesions existing at the time of the accident. Cases of violation of the integrity of insulating personal protective equipment were described. Reports on accidents “with spillage” and “without spillage” (as stated in the Russian normative documents) have not been found. Among the investigated cases, most are associated with violations of various biological safety requirements.
Analysis of the Epizootiological and Epidemiological Situation on Anthrax in the World in 2023, the Forecast for 2024 in the Russian Federation
Authors: Ryazanova A. G. , Skudareva O. N. , Gerasimenko D. K. , Golovinskaya T. M. , Logvin F. V. , Aksenova L. Yu. , Semenova O. V. , Eremenko E. I. , Pechkovsky G. A. , Oleynikova K. A. , Nikitina A. V. , Kulichenko A. N.
Published: October 2024

The results of a review of the epizootiological and epidemiological situation on anthrax around the world in 2023 and the forecast of morbidity in the Russian Federation in 2024 are presented. In 2023, a complication of the epizootiological and epidemiological situation on this infection was recorded in Russia. Seven outbreaks of anthrax with infection of 14 farm animals and 19 humans were registered in five constituent entities of three federal districts (Volga, Siberian, and Central Federal Districts). Epizootiological and epidemiological instability due to the anthrax was detected in four neighboring countries: Azerbaijan, Kazakhstan, Kyrgyzstan, and Tajikistan. Anthrax among farm and wild animals was recorded in Africa, Asia, Europe, and North America. Cases of human disease were reported in Africa, Asia and Europe. Human infections occurred during contact with infected animals, products of animal origin, consumption of meat from sick/fallen livestock and wild animals. The incidence rate of anthrax in animals and humans in Russia in 2024 will correlate with the completeness of registration and specific immunization coverage of susceptible animals and persons from groups with high occupational risk of infection. With proper implementation of all preventive measures and effective surveillance of infection, anthrax incidence in the entities of the Russian Federation will amount to isolated cases.
Effect of Azoximer Bromide on Individual Genomic and Proteomic Characteristics of the Strain during Cultivation of Yersinia pestis EV NIIEG
Authors: Goncharova A. Yu. , Bugorkova S. A. , Abdrashitova A. S. , Shcherbakova N. E. , Krasnov Ya. M. , Germanchuk V. G. , Devdariani Z. L. , Shvidenko I. G. , Shchukovskaya T. N.
Published: October 2024

The aim of the work was to study the effect of the azoximer bromide immunoadjuvant (polyoxidonium, PO) on certain molecular-genetic and proteomic properties of Yersinia pestis EV NIIEG strain, when added to the culture medium. Materials and methods. Y. pestis EV NIIEG was grown at 28 °C for 48 hours on LB agar pH 7.2 (Miller), with and without PO (EV+PO). Whole-genome sequencing of EV and EV+PO strains was performed on the Ion S5 XG generation II platform. Whole-genome SNP analysis and search for marker SNPs were conducted in the Wombac 2.0 program. Mass-spectra of Y. pestis EV extracts and EV+PO cells were recorded using a Microflex LT mass spectrometer. Protective properties of the test cultures were evaluated by the integral ImD50 index in BALB/c mice when infected with Y. pestis 231(708). Results and discussion. Comparative analysis has not revealed deletions, insertions and single nucleotide polymorphisms in the structure of Y. pestis EV+PO strain genome leading to a violation of the production of pathogenicity, immunogenicity and ensuring the vital activity factors of the plague pathogen. The MALDI-TOF mass spectrometry has shown that Y. pestis EV+PO strains changed the intensity of 22 % of the total number of peaks in the range of 2000–20000 Da. Most of the altered peaks in the UniProtKB protein bank belong to uncharacterized proteins and metabolic proteins. At the same time, the ImD50 was 2–3.3 times lower in cultures grown with the addition of PO than in Y. pestis EV. Thus, the addition of PO to Y. pestis EV NIIEG culture medium does not cause changes in the genes of pathogenicity and vital activity support factors of plague pathogen, but modulates its protein profile, which is accompanied by an increase in the protective potential of the EV vaccine strain.
Phylogeny and Characteristics of Yersinia pseudotuberculosis strains from the Aksai High-Mountain and Balkhash Desert Foci of Plague
Authors: Eroshenko G. A. , Dzhaparova A. K. , Nikiforov K. A. , Sidorin A. S. , Kukleva L. M. , Chervyakova N. S. , Krasnov Ya. M.
Published: October 2024

The aim of the study is to obtain a comprehensive characterization of Yersinia pseudotuberculosis strains from the Aksai high-mountain and Balkhash desert foci of plague in Central Asia. Materials and methods. Investigation of the cultural, morphological and biochemical properties of the examined strains was carried out using conventional methods of laboratory diagnostics. Ion S5 XL System (Thermo Fischer Scientific) was used for whole-genome sequencing. Data processing and sequence assembly of raw reads de novo were performed using Ion Torrent Suite software package 5.12 and Newbler gsAssembler 2.6 (454 Life Sciences). To search for marker SNPs, the Wombac 2.0 program based on the BioLinux 8.0 operating system was applied. When constructing the dendrogram, the Maximum Likelihood method, the PhyML 3.1 program and the HKY85 model were used. Results and discussion. The studied strains from the Aksai high-mountain and Balkhash desert foci have properties characteristic of the Y. pseudotuberculosis species, are prototrophs and do not contain the pYV plasmid. According to phylogenetic analysis based on identified 109,641 core SNPs, they are closely related to strains of the pseudotuberculosis microbe from the Sarydzhaz high-mountain focus in the Kyrgyz Republic and the Republic of Kazakhstan, as well as to the O:3 serovar strain from Turkmenistan. The cluster of these Central Asian strains on the phylogenetic tree of Y. pseudotuberculosis is localized next to strains of the pseudotuberculosis microbe of O:3 serovar from various regions of the world. The results obtained testify to the circulation of a separate phylogeographic population of Y. pseudotuberculosis in the territory of natural plague foci in Central Asia. The characterized Y. pseudotuberculosis strains and their whole genome sequences can be used as reference ones for the Aksai and Balkhash natural plague foci of Central Asia.
Распространение вариантов вируса SARS-COV-2, вызывающих интерес (VOI) и находящихся под наблюдением (VUM), на основе количества их геномов, депонированных в базу данных GISAID за неделю с 28 сентября по 04 октября 2024 г.
Authors: Чумачкова Елена , Дмитриева Людмила , Краснов Ярослав , Осина Наталия , Зимирова Анастасия , Иванова Александра , Карнаухов Игорь , Караваева Татьяна , Щербакова Светлана , Кутырев Владимир
Published: October 2024

В обзоре представлена информация по циркулирующим в настоящее время вариантам вируса SARS-COV-2 Omicron вызывающих интерес (VOI) и находящихся под наблюдением (VUM), геномные последовательности которых размещены в международной базе данных GISAID за неделю с 28 сентября по 04 октября 2024 г.
Chronic Kidney Disease (CKD): A Growing Medical Issue
Authors:   Department of Nuclear Medicine and Molecular Imaging, Neurospinal & Medical Institute, Karachi, Pakistan., Kamal Shahid
Published: October 2024

With an annual growth rate of more than 2.08%, the 231 million population of Pakistan is set to surpass 330 million by 2030 [1]. The country’s health system continues to be plagued by a paucity of resources, poor governance, a poor health informa- tion management system, corruption in the healthcare industry, and a dearth of skilled workers. The sector suffers from meager health budget allocations [2]. Kidney diseases, one of the lead- ing causes of death and disability, get further impetus due to an increasingly high prevalence of Diabetes Mellitus that further accentuates CKD [3]. The frequency of DM is set to increase by over 150% between 2000 and 2030 in South Asia [4].