bluuebunny/crossref_metadata_2025 huggingface dataset:
The effect of fumaric acid esters and dithranol on acanthosis and hyperproliferation in psoriasis vulgaris.
Authors: Bacharaçh-Buhles M , Röchling A , El Gammal , Altmeyer P.
Published: June 2023

In this study we investigated the histological changes, regression of acanthosis and rate of proliferation, that accompany the healing of psoriatic lesions after fumaric acid esters and dithranol treatment. Biopsies were taken before and during therapy as well as from neighbouring untreated, clinically uninvolved skin and healthy, non-psoriatic volunteers. Specimens were assessed using computer-supported image analysis and immunohistology. The parameters primarily examined were the height of the rete pegs and of the epithelium above the papillary body, the rate of proliferation, the actual number of cells in the two epidermal compartments and the cellular density in the epidermis. Both fumaric acid esters and dithranol reduce the degree of acanthosis
Neuropeptide- and capsaicin-induced histamine release in skin monitored with the microdialysis technique.
Authors: Huttunen M , Harvima IT , Ackermann L , Harvima RJ , Naukkarinen A , Horsmanheimo M.
Published: June 2023

Mast cells are thought to be involved in neurogenic inflammation in skin, and numerous neuropeptides are known to degranulate mast cells. We monitored histamine release in skin in situ with the microdialysis method after skin challenge with neuropeptide injections (10 microM substance P, vasoactive intestinal peptide and calcitonin gene-related peptide), capsaicin injection (30 microM) and 0.1% capsaicin cream with a moist compress. Fractions were collected for 15 min each at 3.0 microliter/min. One hour after insertion of the probe, the baseline histamine level was 4.5 +/- 4.5 nM (mean +/- SD, n = 20). Substance P (250 pmol) induced histamine release peak (66.1 +/- 52.5 nM, n = 8) in the 0-15 or 15-30 min fraction. Thereafter, the histamine concentration declined steadily and rapidly and no second rise was observed. A single substance P injection was sufficient to induce major histamine release in three out of four experiments
Levels of fluconazole in normal and diseased nails during and after treatment of onychomycoses in toe-nails with fluconazole 150 mg once weekly.
Authors: Faergemann J , Laufen H.
Published: June 2023

Thirty-six patients with onychomycoses of their toe-nails were included in a double-blind, parallel-group comparative study of fluconazole 150 mg once weekly and griseofulvin 1,000 mg once daily for 12 months, or earlier if cured. Every month during treatment and in cured patients 3 and 6 months after stop of treatment one toe-nail was clipped and serum samples were taken. In patients treated with fluconazole the concentration of fluconazole was measured in serum and nails. We found a very high concentration of fluconazole in nails (peak 8.54 micrograms/g) and the nail concentration was statistically significantly higher than serum concentrations (p < 0.001). In cured patients fluconazole was still present in high concentrations 3 (1.7 micrograms/g) and 6 (1.4 micrograms/g) months after stop of treatment. These results indicate that fluconazole should be effective in the treatment of onychomycosis in a dose of 150 mg once weekly. The results also indicate that the treatment period could be shortened because fluconazole is still present in high concentrations 6 months after stop of therapy. The concentration of fluconazole found in nails is much higher than that found in the case of terbinafine and itraconazole, indicating that fluconazole should be at least as effective as these drugs in the treatment of tinea unguium.
Naevus lipomatosus cutaneous superficialis: overlap with connective tissue naevi.
Authors: Orteu CH , Hughes JR , Rustin MH.
Published: June 2023

No abstract available
Hepatitis C virus and sexual transmission.
Authors: Worm AM , Westh H , Kvinesdal BB , Wantzin PS.
Published: June 2023

No abstract available
Penile metastases from bladder carcinoma.
Authors: Filosa G , Bugatti L , Peris K , Chimenti S.
Published: June 2023

No abstract available
Generalized granuloma annulare showing the unusual clinical feature of marked swelling of the hands.
Authors: Kunigvki S , Kanda S.
Published: June 2023

No abstract available
Impaired expression of stem cell factor in dermatofibroma fibroblasts.
Authors: Yamamoto T , Katayama I , Nishioka K.
Published: June 2023

The number of mast cells is increased in dermatofibroma lesions and plays a role in the induction of fibrosis or its proliferation. We have investigated stem cell factor expression in solitary and multiple dermatofibromas by immunohistochemical straining. We also analyzed messenger RNA (mRNA) expression of stem cell factor in dermatofibroma tissues and cultured fibroblasts derived from dermatofibromas, using reverse transcriptase polymerase chain reaction. We found immunoreactive stem cell factor in keratinocytes, dermal fibroblasts, melanocytes and hair follicles. Unexpectedly, a reduced expression of immunoreactive stem cell factor in dermatofibroma fibroblasts was observed in solitary and multiple type. However, mRNA expression of stem cell factor was detected both in the dermatofibroma tissue and cultured dermatofibroma-derived fibroblasts. We speculate that the altered expression of stem cell factor of tumor cells in dermatofibroma lesions can be associated with the tumor cell proliferation and induction of dermatofibroma.
The vitronectin receptor alpha-V beta-3, contrary to ICAM-1, is not modulated by interferon-gamma and tumour necrosis factor-alpha on melanoma cell lines.
Authors: Boccaletti V , Temponi M , Wang Z , Manganoni AM , Marcelli M , Maio M , Ferrone S , De Panfilis G.
Published: June 2023

A correlation was recently shown between expression of the vitronectin receptor (VnR) and the tumorigenic capacity of cultured human melanoma cell lines. On the other hand, modulation of VnR expression by interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) was observed on different non-melanoma cell lines. We tested IFN-gamma, TNF-alpha and interleukin-2 (IL-2), which are presumably released by infiltrating leukocytes in the melanoma lesional environment, on three melanoma cell lines. The VnR expression was assessed using FACS analysis and radioimmunolabelling. The VnR did not show any modulation after treatment with any of the cytokines tested. By contrast, the expression of the intercellular adhesion molecule-1 (ICAM-1), tested as control, on five melanoma cell lines, was greatly enhanced by IFN-gamma and TNF-alpha. Thus, some host cytokines may preferentially induce melanoma cells to express ICAM-1 (which can increase host cytotoxic response against melanoma), other than the VnR (which instead might contribute to melanoma metastasis).
Cyclophosphamide and interleukin-12 synergistically upregulate the acquisition of allergic contact dermatitis in the mouse.
Authors: Maguire Jr HC
Published: June 2023

Cyclophosphamide given before allergen and recombinant interleukin-12 administered at the time of allergic sensitization substantially increase the acquisition of allergic contact dermatitis in the mouse. Since their immunoadjuvant mechanisms appeared different, it seemed probable that combining cyclophosphamide pretreatment with interleukin 12 administration would result in a more intense allergic contact dermatitis than when either agent was used alone. This was tested in different groups of mice sensitized to dinitrofluorobenzene or to oxazolone. Consistently, immunopotentiation of allergic contact dermatitis was significantly greater with the two immunoadjuvants than with either alone. This immunoadjuvant combination is likely to find use in immunization protocols designed to induce a Th-1 helper cell response.
Effect of oral terbinafine treatment on cyclosporin pharmacokinetics in organ transplant recipients with dermatophyte nail infection.
Authors: Jensen P , Lehne G , Fauchald P , Simonsen S.
Published: June 2023

Eleven patients with either kidney, heart or lung transplants, immunosuppressed with cyclosporin A, and with culture-proven dermatophyte toe nail infection, were given 250 mg terbinafine orally daily for 12 weeks. No changes in cyclosporin A dosage were made. A statistically significant decrease in mean specific cyclosporin A blood trough levels was found at 4, 8 and 12 weeks. No other statistically significant changes in the pharmacokinetic profile of cyclosporin A were seen. Terbinafine possibly induces a cyclosporin A metabolic degradation, which, however, is of little clinical significance. Terbinafine treatment is a safe therapeutical option in cyclosporin A-treated patients with dermatophyte nail infection. Cyclosporin A levels should be controlled during treatment.
Expression of gastrin-releasing peptide receptor in human skin.
Authors: Staniek V , Misery L , Peguet-Navarro J , Sabido O , Cuber JC , Dezutter-Dambuyant C , Claudy A , Schmitt D.
Published: June 2023

Bombesin-related peptides are expressed in the skin of batrachians and mammals. As gastrin-releasing peptide belongs to this family, we searched for the presence and distribution of gastrin-releasing peptide receptors (GRPr) in the skin of healthy human adults by immunohistochemistry, flow cytometry and electron microscopy. The results indicated that GRPr are expressed on nerves and vessels in the dermis, on eccrine sweat glands, sebaceous glands and erector pili muscle. Within epidermis, staining was localized only on basal and suprabasal layer cells, or in the whole epidermis, according to the samples studied. Interestingly, suprabasal epidermal dendritic cells occasionally showed a strong labelling. Some of these epidermal dendritic cells were identified as Langerhans' cells by immunoelectron microscopy studies. Flow cytometry analysis of crude epidermal cell suspensions resulted in the expression of GRPr on about 43% of the cells. Therefore, we investigated whether human GRPr could modulate Langerhans' cells antigen-presenting functions. For this purpose, we added increasing concentrations of GRP (10(-12) to 10(-5) M) to mixed epidermal cell lymphocyte reactions. Allogeneic T-cell proliferation was not significantly modified when added to GRP-pretreated epidermal cells. In conclusion, we demonstrated the presence of GRPr in human skin, suggesting that GRP may modulate epidermal cell functions but does not modify antigenic presentation.
Evaluation of port wine stains by laser Doppler perfusion imaging and reflectance photometry before and after pulsed dye laser treatment.
Authors: Troilius AM , Ljunggren B.
Published: June 2023

Treatment of choice for congenital capillary malformations of the port wine stain type is presently the pulsed dye laser. Although treatment results have usually been excellent or good, a few patients respond less well. Looking for a tool to predict and monitor the treatment we used laser Doppler perfusion imaging and reflectance photometry. Measurements with laser Doppler perfusion imaging were performed in 19 patients initially and after 1-3 treatments and with reflectance photometry initially and after 1-6 treatments. Before treatment, 15 of the patients had an increased bloodflow within the port wine stain in comparison with normal contralateral skin. After the laser treatments, 15 of 18 patients had decreased bloodflow within the lesion and all 18 had surrounding hyperemia. Reflectance photometry showed a successive increase in blanching and predicted within 6 weeks of the first treatment the eventual clinical result. The bloodflow, as measured with laser Doppler perfusion imaging, did not correlate well with the photometrically registered erythema. Reflectance photometry is a useful objective tool, which early in the treatment course indicates whether laser therapy will be successful. Laser Doppler perfusion imaging is less helpful in monitoring patients but may be of use in the study of port wine stain pathophysiology.
Autosomal recessive cutis laxa syndrome. A case report.
Authors: Jung K , Ueberham U , Hausser I , Bosler K , John B , Linse R.
Published: June 2023

Congenital cutis laxa (CCL) is a rare, genetically heterogeneous connective tissue disorder, manifested by loose, hanging skin, giving the appearance of premature aging. We report a 6-year-old female child with autosomal recessive CCL type III, to assess possible correlations between clinical, ultrastructural, cellular and biochemical features. Morphological aberrations of the elastic and collagen tissue, increased collagen I mRNA expression associated with increased protein synthesis and increased collagenase gene expression of the cutis laxa fibroblasts could be established. Our results suggest that CCL is not only a disease of the elastic fibers of the connective tissue but also of the collagen fibers, with a central role of the fibroblast.
Sun exposure and sunscreen use among sunbathers in Denmark.
Authors: Stender IM , Andersen JL , Wulf HC.
Published: June 2023

Eight hundred and eight sunbathing Danes were interviewed in order to register the duration of sun exposure and sunscreen use. The mean sun exposure time, 203 min, was found to be independent of sex, age and use of sunscreen. Overall, sunscreens were used by 65%, 73% of the females and 52% of the males. The median sun protection factor used by subjects older than 10 years was 5
Skin disease and age-related cataract.
Authors: Phillips CI , Donnelly CA , Clayton RM , Cuthbert J.
Published: June 2023

Dermatological conditions and treatments were analysed in a study comparing cataract patients and stringently matched controls. One thousand patients were taken from the cataract waiting list of a specialist eye hospital. For each patient a matched control of the same gender, half-decade of age, and family doctor but without cataract was selected. Venepunctures and eye examinations were performed on both patients and controls
Granuloma annulare: clinical and laboratory findings in a pediatric group of patients.
Authors: Patrizi A , Neri I , Guerrini V , Marini R , Fiorillo L.
Published: June 2023

No Abstract available.
Tri-chloroacetic acid: a cause of vulvar vestibulitis.
Authors: Nunns D , Mandal D.
Published: June 2023

No Abstract available.
Measurement of sodium lauryl sulfate-induced skin irritation.
Authors: Treffel P , Gabard B.
Published: June 2023

Besides visual evaluation, skin irritation induced by sodium lauryl sulfate (SLS) may be characterized by bioengineering measurements, such as skin colour reflectance, transepidermal water loss (TEWL) or hydration. Short application times or low concentrations of the irritant usually do not modify the visual aspect of the skin, and the measurements described above are unchanged or only slightly altered. We were looking for a suitable method to measure cutaneous changes not detectable by usual bioengineering procedures. Therefore these measurements were compared to those of dynamic function testing of the stratum corneum, namely sorption-desorption and moisture accumulation tests. Different concentrations of SLS (0.1%, 0.5%, 2.5%), application times (15 min, 24 h) and times of testing (1 h, 24 h after patch removal) were investigated on the ventral forearm of human subjects. When SLS was applied for a short period (15 min), 1 h after patch removal skin colour, TEWL and hydration were not modified, while increases in hygroscopicity, water-holding capacity and water accumulation were detected depending on the applied concentration. Increase of hygroscopicity was closely correlated with the alteration of epidermal barrier function (TEWL). We demonstrated that sorption-desorption and moisture accumulation tests performed on SLS-treated areas for a short period, without visible modifications, could evaluate changes of the stratum corneum properties. We consider these tests as useful complementary methods to skin colour, TEWL and hydration measurements, particularly in the detection of subclinical skin injuries.
Topical treatment by urea reduces epidermal hyperproliferation and induces differentiation in psoriasis.
Authors: Hagemann I , Proksch E.
Published: June 2023

The mechanisms of action of urea-containing ointments in the treatment of eczema, ichthyosis and psoriasis are only partly known and related to proteolysis and keratinolysis. In this study, we have examined the effects of topical urea on epidermal proliferation and differentiation in 10 patients with psoriasis. Plaque type lesions were treated for 2 weeks with an ointment containing 10% urea, with the vehicle alone, or left untreated. Clinical score, hydration of the stratum corneum, transepidermal water loss (TEWL), and immunohistochemical studies were performed. Epidermal proliferation was assessed using the Ki-S3 proliferation-associated nuclear antigen. For epidermal differentiation antibodies against involucrin and against keratins CK 5, 6, 17 and CK 1, 5, 10, 14 were used. The patients showed a reduction of the clinical score (> 50%), a 2-fold increase in stratum corneum hydration (p < 0.01), and a small decrease in TEWL (N.S.) on the urea- treated compared to the untreated site. Light microscopy studies revealed a 29% reduction in epidermal thickness (p < 0.01)
Urinary glycosaminoglycans excretion in Graves' disease.
Authors: Priestley GC , Aldridge RD , Hurel S.
Published: June 2023

Urinary excretion of glycosaminoglycans was measured in 10 patients with pretibial myxoedema, 7 of whom also had thyroid-associated ophthalmopathy, and in 3 additional patients with ophthalmopathy but no skin changes. Total uronic acid excretion was raised above control levels in only 2 patients, who had both eye and skin disease of recent onset. In these patients excretion was initially three times the control level but declined sharply in subsequent months. This decline was in the absence of effective treatment or spontaneous improvement and would appear to reflect the natural history of the disease. These data show that although glycosaminoglycans excretion may be disturbed in Graves' disease, it provides an unreliable reflection of clinical status and of the effectiveness of treatment.
Købner reaction induced by photodynamic therapy using delta-aminolevulinic acid. A case report.
Authors: Stender IM , Wulf HC.
Published: June 2023

We report a case of psoriasis reactivation following photodynamic therapy with delta-aminolevulinic acid. An 84-year-old women received several UVB treatment sessions because of severe psoriasis at her crura in the period from 1960 to 1990. Since then her psoriasis had been inactive. In 1994 she was admitted to our out-patient clinic because of multiple dysplastic lesions as well as multiple carcinomas at her crura. Surgical intervention was impossible and retinoids were not tolerated. She was treated with photodynamic therapy, which provoked a Købner reaction followed by a severe reactivation of the otherwise inactive psoriasis. Whether photodynamic therapy is used for the treatment of multiple dysplastic lesions in a psoriasis patient or for the treatment of psoriasis it seems important to be aware of this adverse effect. This is the first report of Købner reaction following photodynamic therapy.
Keratosis lichenoides chronica with chronic hepatitis: a coincidence?
Authors: Marschalkó M , Papp I , Szalay L , Hársing J , Horváth A.
Published: June 2023

No abstract available.
Dramatic vasculopathy in a patient with antiphospholipid syndrome.
Authors: Lalova A , Popov I , Dourmishev A , Baleva M , Nikolov K.
Published: June 2023

No abstract available.
Schnitzler's syndrome (urticaria and macroglobulinemia): evolution to Waldenström's disease is not uncommon.
Authors: Machet L , Vaillant L , Machet MC , Reisenleiter M , Goupille P , Lorette G.
Published: June 2023

No abstract available.
Abnormal desmoglein expression by squamous cell carcinoma cells.
Authors: Harada H , Iwatsuki K , Ohtsuka M , Han GW , Kaneko F.
Published: June 2023

Abnormal expression of cell adhesion molecules and related proteins has been observed in various carcinoma cells. We compared expression patterns of desmosomal cadherins, E-cadherin, and cytoplasmic plaque proteins of four different human squamous cell carcinoma cell lines and in vivo squamous cell carcinoma cells with those of normal human keratinocytes. Unlike normal human keratinocytes, the squamous cell carcinoma cells, both in culture and in vivo, exhibited diminished or unusual expression of desmoglein 3 and desmoglein 1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively. Abnormal expression of E-cadherin and cytoplasmic plaque proteins such as desmoplakin and plakoglobin was also observed. Western blotting study demonstrated that three squamous cell carcinoma cell lines expressed two desmogleins with a predominant 150 kDa molecule, and a minor 130 kDa one. Although these molecular sizes were similar to those of cultured normal human keratinocytes, the 130 kDa desmoglein, which usually carries pemphigus antigenic epitopes, was weakly or negatively reactive with pemphigus vulgaris serum. One squamous cell carcinoma cell line showed a doublet of 140 and 145 kDa bands in addition to the 130 kDa band. All the carcinoma cell lines constantly expressed desmoglein 2 and desmoglein 3 mRNA, whereas cultured normal human keratinocytes always expressed desmoglein 1 and desmoglein 3 mRNA, with or without desmoglein 2 mRNA. These findings indicate that the squamous cell carcinoma cells revealed abnormal expression of desmoglein isoforms, which may be related to tumor cell kinetics such as cell invasion and metastasis.
Cytokines in alopecia areata: contrasting cytokine profiles in localized form and extensive form (alopecia universalis).
Authors: Teraki Y , Imanishi K , Shiohara T.
Published: June 2023

Recent studies have suggested that cytokines play a critical role in the pathophysiology of alopecia areata
Photoprotection in vitiligo and normal skin. A quantitative assessment of the role of stratum corneum, viable epidermis and pigmentation.
Authors: Gniadecka M , Wulf HC , Mortensen NN , Poulsen T.
Published: June 2023

Pigmentation, stratum corneum and viable epidermis are considered to be the main factors protecting against ultraviolet radiation. We quantitatively investigated the degree of photoprotection provided by these structures in vitiligo and adjacent normally pigmented skin. In 14 patients 61 MED tests were performed in vitiligo and adjacent normally pigmented skin using a solar simulator. The thickness of stratum corneum and viable epidermis was determined from frozen skin sections, and pigmentation was calculated by measuring skin reflectance at 555 nm and 660 nm. To analyse photoprotection, the UV dose necessary to evoke erythema was regressed against the thickness of stratum corneum and viable epidermis, pigmentation and the erythema grade in the MED test. By analysing regression coefficients we found that stratum corneum was the main photoprotective factor not only in vitiligo but also in normally pigmented skin. The effect of pigmentation in normal skin was slightly less prominent. Stratum corneum was thicker in vitiligo than in normally pigmented skin. However, the photoprotection due to stratum corneum was similar in both groups because significantly less photoprotection was achieved per thickness unit of stratum corneum in vitiligo than in normal skin. Neither in vitiligo nor in normally pigmented skin did the photoprotection depend on viable epidermis. Our data quantitatively document the importance of stratum corneum and pigmentation. Hyperkeratosis in vitiligo offers just as efficient photoprotection as does the normal stratum corneum in pigmented skin.
Antiproliferative effect of pentoxifylline on psoriatic and normal epidermis. In vitro and in vivo studies.
Authors: Gilhar A , Grossman N , Kahanovicz S , Reuveni H , Cohen S , Eitan A.
Published: June 2023

Psoriasis is characterized by abnormal cell proliferation, inflammation and increased biosynthesis of various cytokines. The inhibitory effect of pentoxifylline on some cell functions has been reported widely. This property of pentoxifylline prompted an investigation of its possible role in controlling psoriasis. In the in vitro study normal human keratinocytes proliferation was determined and formation of cornified envelopes was assayed following treatment with pentoxifylline. The in vivo experiment consisted of nude mice grafted with psoriatic or normal skin treated with tetradecanyl phorbol 13 acetate. At the end of the treatment period, the grafts were excised and assessed for acanthosis and labelling index. The in vitro study showed that continuous exposure of normal human keratinocyte cultures to pentoxifylline resulted in a significant dose-dependent inhibition of proliferation, and in induction of cornified envelope formation. The in vivo experiments showed a significant reduction of epidermal thickness and of labeling index in psoriatic and tetradecanyl phorbol 13 acetate-treated normal skin, as compared to the initial values.
Change of skin roughness due to lowering air humidity in climate chamber.
Authors: Eberlein-König B , Spiegl A , Przybilla B.
Published: June 2023

Rough skin ("dry skin") is characteristic of atopy and thought to be a pre-stage of atopic eczema. Atopic eczema frequently deteriorates in winter, which may be related to low air humidity during the heating period. We have assessed skin roughness before and after decreasing air humidity to 30% in a climatic chamber. Replicas of clinically non-inflamed and not scaling skin from 10 patients with atopic eczema and 10 controls were taken before and after lowering air humidity for 3 h and were analyzed for the roughness parameters Ra, RZ(DIN) and RZ(ISO). After exposure to low air humidity there was a significant increase of RZ(DIN) from 61.5 microns to 66.9 microns (p < 0.05) and of RZ(ISO) from 63.8 microns to 66.4 microns (p < 0.05) in patients with atopic eczema, whereas no significant change occurred in controls. Development of skin roughness over 3 h under natural indoor environmental conditions did not indicate spontaneous variations. These quantitative data show that a short period of exposure to low air humidity increases skin roughness and may particularly influence the condition of patients with atopic eczema.
Q-switched ruby laser application is safe and effective for the management of actinic lentigo (topical glycolic acid is not).
Authors: Kopera D , Hohenleutner U , Landthaler M.
Published: June 2023

A study was designed to evaluate the effectiveness of single q-switched ruby laser exposure to erase actinic lentigo. A peeling fluid containing glycolic acid designed to treat lentigines was evaluated comparatively. Ten female patients presenting with actinic lentigines on the forearms and dorsal aspects of their hands were treated with the q-switched ruby laser on the right side. Single ruby laser irradiation of actinic lentigines on the dorsal aspects of forearms and hands caused transient crusting due to exfoliation of the epidermal surface, which generally lasted for 2 weeks. Four weeks after treatment total fading of the lesions was evident. Topical application of peeling fluid applied on the left forearms caused burning sensations, local irritation and superficial scaling but could not clear the lentigines. A single course of q-switched ruby laser exposure is safe and efficient for the management of actinic lentigines, as it completely clears these obvious signs of aging. Topical treatment of lentigines using a commercial peeling solution leads to moderate or severe irritation and is ineffective.
Burning mouth syndrome: the role of contact hypersensitivity.
Authors: Virgili A , Corazza M , Trombelli L , Arcidiacono A.
Published: June 2023

The burning mouth syndrome is characterized by an unpleasant sensation of burning in the oral cavity, without clinical signs. Causal factors may be psychogenic, systemic or local. The aim of the study was to determine the significance of contact allergy in the pathogenesis of burning mouth syndrome. Fifteen patients with burning mouth syndrome were studied through anamnesis and laboratory analysis. Epicutaneous patch tests were performed with the Italian standard series (GIRDCA - Gruppo Italiano di Ricerca Dermatiti da Contatto ed Ambientali), preservative and dental series. The same tests were carried out in 12 healthy age- and sex-matched subjects. The number of patients affected by burning mouth syndrome with a positive reaction to patchtesting was 6 out of 15, while the number of allergic patients in the control group was 3 out of 12. No association could be found between positive reaction at patchtesting and exposure to allergens. Contact allergy in burning mouth syndrome seems not to play a primary role
Pseudoallergen-free diet in the treatment of chronic urticaria.
Authors: Haustein UF.
Published: June 2023

No abstract available
Coexistence of subcutaneous sarcoidosis of the sole and scar sarcoidosis.
Authors: Ohi T , Saijo S , Tagami H.
Published: June 2023

No abstract available
Gianotti-Crosti syndrome in an adult patient following a recently acquired Epstein-Barr virus infection.
Authors: Mempel M , Abeck D , Bye-Hansen T , Ring J.
Published: June 2023

No abstract available
Flare-up at contact allergy sites in a gold-treated rheumatic patient.
Authors: Möller H , Larsson A , Björkner B , Bruze M , Hagstam A.
Published: June 2023

Contact allergy to gold sodium thiosulfate and gold sodium thiomalate was established by skin testing in a rheumatic patient intended for gold therapy. An intramuscular test dose of gold sodium thiomalate (Myocrisin) induced a flare-up of previously positive epicutaneous and intradermal test reactions, with a histological and immunohistochemical picture compatible with an allergic contact dermatitis. Since gold allergy is frequent, the cutaneous side-effects of gold therapy ("gold dermatitis") may be explained by such an immunopathological reaction.
A therapeutic approach to erythrodermic psoriasis: report of a case and a discussion of therapeutic options.
Authors: Van der Vleuten CJ , Gerritsen MJ , Steijlen PM , De Jong EM , Van de Kerkhof PC.
Published: June 2023

In this case report a patient with therapeutically recalcitrant erythrodermic psoriasis is presented. After various attempts with several major therapies in this patient, the first substantial improvement was achieved using the combination of cyclosporine and calcipotriol, followed by the combination of UVB and calcipotriol. The therapeutic options for severe psoriasis are discussed, and since combined approaches seem to be an attractive alternative for severe psoriasis, mechanisms of synergy of combined therapeutic approaches are hypothesised.
Response of the clinically uninvolved skin of psoriatic patients to tape stripping during acitretin treatment.
Authors: Gerritsen MJ , Van Pelt JP , Van de Kerkhof
Published: June 2023

The aromatic retinoids etretinate and acitretin are widely used in the systemic treatment of severe psoriasis. The purpose of the present investigation was to further elucidate the mode of action of acitretin on abnormal keratinization and epidermal hyperproliferation in an in vivo model. Studies on the interference of acitretin with epidermal hyperproliferation and abnormal keratinization in psoriatic plaques are difficult to interpret, as acitretin-induced changes might be due to direct effects of acitretin or be the indirect effect of retinoid-induced modulation of cutaneous inflammation. Using an immunohistochemical assessment, we examined the in vivo effect of systemic acitretin ( > 35 mg daily) on the expression of filaggrin, involucrin, and on the recruitment of cycling epidermal cells, in the tape-stripped uninvolved skin of psoriatic patients, a model which provides the opportunity to study epidermal regeneration in the absence of significant accumulation of T-lymphocytes. During acitretin therapy and 3 weeks after withdrawal of acitretin, we took biopsies from uninvolved skin following tape-stripping in 6 patients with psoriasis. Six patients with psoriasis who had never used acitretin served as controls. We did not observe a Koebner response in our patients after tape stripping. Filaggrin expression was decreased, while the recruitment of cycling epidermal cells and the involucrin expression were increased in the biopsies taken from patients who did not use acitretin. During acitretin treatment, however, the filaggrin expression was similar, whereas the Ki-67 positive nuclei and the involucrin expression showed a statistically significant decrease compared to those parameters in the patients who did not use acitretin. Our findings indicate that epidermal hyperproliferation and abnormal keratinization are modulated directly by acitretin.
Scrotal angiokeratoma in a young man.
Authors: Hisa T , Taniguchi S , Goto Y , Teramae H , Osato K , Kakudo K , Takigawa M.
Published: June 2023

No abstract available.
Effect of topical laurocapram (Azone) on the in vitro percutaneous permeation of sodium lauryl sulfate using human skin.
Authors: Szolar-Platzer C , Patil S , Maibach HI.
Published: June 2023

The probability of simultaneous cutaneous exposure to surfactants and penetration enhancers could occur frequently during routine skin treatment. This study ascertains whether pre-exposure of skin to laurocapram would affect the penetration of a model surfactant, sodium lauryl sulfate (SLS). In vitro experiments with human skin were performed to compare the penetration of SLS after pretreatment with (1) different concentrations of laurocapram, (2) after repeated SLS treatments, (3) untreated controls, and (4) water-control. Pre-exposure to laurocapram enhanced penetration of SLS compared to all other treatments (p < 0.05). Since subsequent pre-exposure of skin to laurocapram increased SLS penetration, the chances of an elevated skin irritation reaction at the exposed site may therefore be possible. Pre-exposure of the skin with SLS did not increase the SLS flux values significantly, compared to the laurocapram pretreated skin. From these results it can be proposed that proper care and precautions may be necessary after exposure of skin to laurocapram and also to various other percutaneous enhancers. Further in vivo correlations are essential to define the clinical implications of this study, especially as related to irritant dermatitis.
Discoid lupus erythematosus during treatment with cyclosporine.
Authors: Di Lernia V , Bisighini G.
Published: June 2023

No abstract available.
Occupational allergic contact dermatitis from epoxy resin in a dental nurse with primary sensitization during cyclosporine treatment.
Authors: Kanerva L.
Published: June 2023

No abstract available.
A new model for assessing the damaging effects of soaps and surfactants on human stratum corneum.
Authors: Shukuwa T , Kligman AM , Stoudemayer TJ.
Published: June 2023

To elucidate the damage to the horny layers of human skin produced by surfactants and soaps, we evaluated the cytological alterations of corneocytes using an in vitro assay. Suction blisters, 8 mm in diameter, were raised on the forearms of young adult Caucasoids. The roofs were cut off and the viable epidermis was removed. The discs of stratum corneum were then agitated for up to 6 h at 60 degrees C in 1% solution of five soap bars of differing irritancy. Additionally, individual examples of anionic, cationic and nonionic surfactants were similarly evaluated. Measurements of corneocytes included: (1) the number released with time (disaggregation), (2) size (swelling) and (3) morphologic degradation. The effects of the cationic and non-ionic surfactants did not differ significantly from those of distilled water. The anionic surfactant caused more release and less swelling and morphological change. The test soaps had vastly different effects on the structural integrity of the stratum corneum. The harsher ones caused greater disaggregation, more swelling and greater morphologic deterioration of corneocytes, whereas the milder ones had less marked effects on these parameters. This model would be a useful screening technique for formulating milder soaps and might also provide insights into the complex modes of action of surfactants on the stratum corneum.
Immunopathological events of adverse cutaneous reactions to coumarin and heparin.
Authors: Hermes B , Haas N , Henz BM.
Published: June 2023

We here describe a female patient with thromboembolic disease, who exhibited allergic reactions to heparin and who developed a large necrotic area on the abdomen when coumarin treatment was instituted. On immunohistology of the necrotic lesion, tumour necrosis factor alpha was markedly expressed, with decreasing intensity towards the central necrotic part of the lesion. Furthermore, endothelial cell adhesion molecule expression was upregulated, particularly in the haemorrhagic zone at the periphery of the lesion. These findings suggest that the pathology of coumarin necrosis is mediated via tumour necrosis factor alpha-associated inflammatory events, after activation of the coagulation pathway due to an inherited or transiently induced, acquired protein C-deficiency. In view of these findings, we propose that patients be treated in the future with tumour necrosis factor alpha antagonists such as pentoxifylline.
Extramammary Paget's disease.
Authors: Burrows NP , Pye RJ.
Published: June 2023

No Abstract available
Pseudotumor cerebri induced by minocycline treatment for acne vulgaris.
Authors: Lewis PA , Kearney PJ.
Published: June 2023

No Abstract available
Influence of epidermal permeability barrier disruption and Langerhans' cell density on allergic contact dermatitis.
Authors: Proksch E , Brasch J.
Published: June 2023

Previously, we have showed that artificial epidermal permeability barrier disruption leads to an increase in epidermal Langerhans' cell (LC) density within 24 h. We now asked if this is accompanied by an enhancement of allergic contact dermatitis. Barrier disruption was induced by acetone on the upper arms in 6 volunteers with known sensitization to nickel, fragrance mix, or p-phenylenediamine. Twenty-four hours after this treatment the relevant allergen was applied without occlusion or with Finn chambers. Twenty-four hours after application of the allergen, clinical grading and transepidermal water loss (TEWL) measurements were performed and biopsies were taken. Immunohistochemical stainings for LCs (anti-CD1a, Leu6) and for epidermal proliferation (Ki-S3) were performed. Open applications of the allergens after acetone pretreatment resulted in strong allergic test reactions. TEWL, which showed a 70% recovery 24 h after acetone treatment, was increased again 4-fold by the allergic test reactions. LC density, which was increased by 80% 24 h after acetone-induced barrier disruption, was further enhanced 2.4-fold in total. Epidermal proliferation showed a 6-fold increase after open application of the allergens. Under patch test conditions after acetone pretreatment very strong bullous reactions were observed. We conclude that the increase in epidermal LC density induced by epidermal permeability barrier disruption is accompanied by an enhanced response in allergic contact dermatitis.
Low-dose (1.25 mg/kg) cyclosporin A: treatment of psoriasis and investigation of the influence on lipid profile.
Authors: Meffert H , Bräutigam M , Färber L , Weidinger G.
Published: June 2023

This study was designed to investigate the therapeutical efficacy of a comparatively low dose cyclosporin A (1.25 mg/kg/day) in the treatment of psoriasis and to assess the influence of cyclosporin A on lipid profiles. In the first, double-blind part of the study, 133 patients with moderate to severe psoriasis were randomized to receive a daily dose of 1.25 or 2.5 mg cyclosporin A/kg or placebo for a period of 10 weeks (period I). Subsequently, the patients entered a second open-label 12-week study (period II), in which the dosage could be increased up to 5.0 mg/kg/day. This was followed by a period of 4 weeks without treatment. After 10 weeks the percentage improvement from baseline in the Psoriasis Area and Severity Index (PASI) was 5.9% on placebo, 27.2% on 1.25 mg/kg/day and 51.0% on 2.5 mg/kg/day cyclosporin A. The final average dose at the end of study period II was 2.99 mg cyclosporin A/kg/day. At this time the PASI was reduced by at least 75% in 63.0% of the patients. From this group of good responders no patient relapsed (PASI > 50% of baseline) during the four weeks after termination of active treatment. No significant effects of the drug on the lipid profiles were detected. We conclude that 1.25 mg cyclosporin A/kg/day is superior to placebo in the treatment of psoriasis vulgaris and that a dose reduction to 1.25 mg/kg/day should be considered in patients responding well to a conventional dose between 2.5 and 5.0 cyclosporin A/kg/day.
A controlled trial of acupuncture in psoriasis: no convincing effect.
Authors: Jerner B , Skogh M , Vahlquist A.
Published: June 2023

Several uncontrolled studies have suggested that acupuncture is an effective treatment for psoriasis. To test this hypothesis, 56 patients suffering from long-standing plaque psoriasis were randomized to receive either active treatment (electrostimulation by needles placed intramuscularly, plus ear-acupuncture) or placebo (sham, 'minimal acupuncture') twice weekly for 10 weeks. The severity of the skin lesions was scored (PASI) before, during, and 3 months after therapy. After 10 weeks of treatment the PASI mean value had decreased from 9.6 to 8.3 in the 'active' group and from 9.2 to 6.9 in the placebo group (p < 0.05 for both groups). These effects are less than the usual placebo effect of about 30%. There were no statistically significant differences between the outcomes in the two groups during or 3 months after therapy. The patient's own opinion about the results showed no preference for 'active' therapy. It was also clear from the answers that the blinded nature of the study had not been discovered by the patients. In conclusion, classical acupuncture is not superior to sham (placebo) 'minimal acupuncture' in the treatment of psoriasis.
Hyperpigmentation of the flexures and pancytopenia during treatment with folate antagonists.
Authors: Jucgla A , Sais G , Berlanga J , Servitje O.
Published: June 2023

No abstract available.
Keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK-syndrome): a rare, autosomal recessive disorder of keratohyaline formation?
Authors: Vahlquist A , Pontén F , Pettersson A.
Published: June 2023

We report a 32-year-old man with an unusual combination of congenital ichthyosis, sclerosing palmoplantar keratoderma with pseudoainhum, and bizarre striate hyperkeratosis in the fixtures, but no systemic involvement. The condition, which improved on oral etretinate therapy, had not appeared previously in the family. On light microscopy the involved epidermis showed marked acanthosis with hypergranulosis and hyperkeratosis. Electron microscopy disclosed numerous large keratohyaline granules in superficial keratinocytes. The clinical picture and histology are virtually identical to those of a Spanish family suffering from an autosomally recessive skin disease of unknown etiology. We hypothesize that the condition is due to a genetic defect in the formation of keratohyaline granules.
Cycloheximide in dermatology.
Authors: Montes LF.
Published: June 2023

No abstract available
Extramammary Paget's disease associated with autoimmune hemolytic anemia.
Authors: Okuyama R , Tagami H.
Published: June 2023

No abstract available
Naevus comedonicus as dermatologic hallmark of occult spinal dysraphism.
Authors: Filosa G , Bugatti L , Ciattaglia G , Salaffi F , Carotti M.
Published: June 2023

No abstract available
Lipo-prostaglandin E1 therapy for livedo reticularis with ulceration.
Authors: Nonaka Y , Sibue K , Shimizu A , Koga T , Toshitani S.
Published: June 2023

No abstract available
Vulvar adenosis after diathermy treatment for condylomas.
Authors: Aloi F , Solaroli C , Pippione M.
Published: June 2023

No abstract available
Evidence that HLA-Cw6 determines early onset of psoriasis, obtained using sequence-specific primers (PCR-SSP).
Authors: Enerbäck C , Martinsson T , Inerot A , Wahlström J , Enlund F , Yhr M , Swanbeck G.
Published: June 2023

Psoriasis vulgaris has previously been shown to associate with certain HLA alleles. HLA-Cw6 is considered to be the primary association, based on calculations of relative risk after serological typing. This association is reportedly more pronounced in early- than in late-onset psoriasis. We performed a PCR-based typing with sequence-specific primers, which has been shown to give a more complete result than serology. Two hundred and one unrelated patients with psoriasis, with a mean age of 40 years, and 77 healthy controls were typed. Two thirds (67%) of the patients were positive for one or two copies of the allele, while the corresponding figure for the control group was 12%. A significant peak for age at onset of 21 or younger was seen for the Cw6 carriers. For patients older than 21 at onset, the frequency of Cw6 was significantly lower
Insulin inhibits tyrosinase activity and 5-S-cysteinyldopa formation in human melanoma cells.
Authors: Benathan M , Labidi F.
Published: June 2023

The effects of insulin on melanogenesis were examined in human Swift melanoma cells. When these cells were grown in a chemically defined culture medium containing insulin (5 microg/ml), they showed a low pigmentation in association with a high activity of glutathione peroxidase (GPO) and a low activity of tyrosinase. In Eagle's minimum essential medium supplemented with foetal calf serum (EMEM-FCS), the Swift cells showed an intense pigmentation in association with a low GPO activity and a high tyrosinase activity. Modulation of GPO activity with sodium selenite had no effect on melanogenesis variables. In contrast, addition of insulin (5 microg/ml) to the EMEM medium led to a marked decrease in tyrosinase activity (p
Rapid cytological diagnosis of primary skin tumours and tumour-like conditions.
Authors: Daskalopoulou D , Gourgiotou K , Thodou E , Vaida S , Markidou S.
Published: June 2023

This study presents the results of fine needle aspiration cytology performed on 1,263 skin lesions which were clinically suspicious for neoplasia. The purpose of the study was to investigate the accuracy of fine needle aspiration cytology for the diagnosis of skin tumours and to assess its clinical value. Twenty-one to 27 Gauge needles were used and the specimens were stained by a quick Giemsa stain. The cytological examination reported 826 primary malignant tumours and 437 benign lesions. Five hundred and thirteen of the cytologically malignant cases and 123 of the benign ones had a subsequent histological examination. The correlation between cytology and histology revealed 6 false positive cytological results and one false negative. Persuaded by our results, we believe that fine needle aspiration cytology can give highly reliable information concerning the histological type or primary skin tumours. It can also detect or exclude relapses of previously treated neoplasms. The procedure is non-traumatic, safe, quick, inexpensive and very well tolerated by the patients.
Is vulvar vestibulitis an inflammatory condition? A comparison of histological findings in affected and healthy women.
Authors: Lundqvist EN , Hofer PA , Olofsson JI , Sjöberg I.
Published: June 2023

Vulvar vestibulitis, as defined by Friedrich, is considered to be inflammatory, despite the fact that the normal histology of this specific area has previously not been characterized. The aim of the present study was to compare the normal histology of the vulvar vestibulum with findings in localized vulvar vestibulitis. Biopsies were taken at the area of the vestibulitis, i.e. at the openings of the Bartholin's duct. Eleven control specimens were examined histologically and compared to 24 specimens obtained from 20 patients. All samples were also tested for human papillomavirus, and they were all negative. In control specimens, as well as in specimens from patients, subepithelial inflammatory cells, sometimes aggregated into lymph follicles and/or small groups of lymphocytes were found. The conclusion is reached that the occurrence of inflammatory cells in vestibular tissue is a normal finding and cannot serve as a histological indicator of vulvar vestibulitis.
Hair loss in children on long-acting gonadotropin-releasing hormone agonist triptorelin treatment.
Authors: Kauschansky A , Lurie R , Ingber A.
Published: June 2023

No abstract available