bluuebunny/crossref_metadata_2025 huggingface dataset:
Developing the Chinese Academic Map Publishing Platform
Authors: Xu Yongming , Lewis Benjamin , Guan Weihe Wendy
Published: December 2019

The discipline of the humanities has long been inseparable from the exploration of space and time. With the rapid advancement of digitization, databases, and data science, humanities research is making greater use of quantitative spatiotemporal analysis and visualization. In response to this trend, our team developed the Chinese academic map publishing platform (AMAP) with the aim of supporting the digital humanities from a Chinese perspective. In compiling materials mined from China’s historical records, AMAP attempts to reconstruct the geographical distribution of entities including people, activities, and events, using places to connect these historical objects through time. This project marks the beginning of the development of a comprehensive database and visualization system to support humanities scholarship in China, and aims to facilitate the accumulation of spatiotemporal datasets, support multi-faceted queries, and provide integrated visualization tools. The software itself is built on Harvard’s WorldMap codebase, with enhancements which include improved support for Asian projections, support for Chinese encodings, the ability to handle long text attributes, feature level search, and mobile application support. The goal of AMAP is to make Chinese historical data more accessible, while cultivating collaborative opensource software development.
HsgNet: A Road Extraction Network Based on Global Perception of High-Order Spatial Information
Authors: Xie Yan , Miao Fang , Zhou Kai , Peng Jing
Published: December 2019

Road extraction is a unique and difficult problem in the field of semantic segmentation because roads have attributes such as slenderness, long span, complexity, and topological connectivity, etc. Therefore, we propose a novel road extraction network, abbreviated HsgNet, based on high-order spatial information global perception network using bilinear pooling. HsgNet, taking the efficient LinkNet as its basic architecture, embeds a Middle Block between the Encoder and Decoder. The Middle Block learns to preserve global-context semantic information, long-distance spatial information and relationships, and different feature channels’ information and dependencies. It is different from other road segmentation methods which lose spatial information, such as those using dilated convolution and multiscale feature fusion to record local-context semantic information. The Middle Block consists of three important steps: (1) forming a feature resource pool to gather high-order global spatial information; (2) selecting a feature weight distribution, enabling each pixel position to obtain complementary features according to its own needs; and (3) inversely mapping the intermediate output feature encoding to the size of the input image by expanding the number of channels of the intermediate output feature. We compared multiple road extraction methods on two open datasets, SpaceNet and DeepGlobe. The results show that compared to the efficient road extraction model D-LinkNet, our model has fewer parameters and better performance: we achieved higher mean intersection over union (71.1%), and the model parameters were reduced in number by about 1/4.
Aldh2 Attenuates Stem Cell Factor/Kit-Dependent Signaling and Activation in Mast Cells
Authors: Kim Do-Kyun , Cho Young-Eun , Song Byoung-Joon , Kawamoto Toshihiro , Metcalfe Dean D. , Olivera Ana
Published: December 2019

Mitochondrial aldehyde dehydrogenase (ALDH2) metabolizes endogenous and exogenous aldehydes and protects cells against oxidative injury. Inactivating genetic polymorphisms in humans are common and associate with alcohol flush reactions. However, whether mast cell Aldh2 activity impacts normal mast cell responses is unknown. Using bone marrow-derived mast cells from Aldh2 knockout mice, we found evidence for a role of mast cell Aldh2 in Kit-mediated responses. Aldh2-deficient mast cells showed enhanced Kit tyrosine kinase phosphorylation and activity after stimulation with its ligand (stem cell factor) and augmentation of downstream signaling pathways, including Stat4, MAPKs, and Akt. The activity of the phosphatase Shp-1, which attenuates Kit activity, was reduced in Aldh2−/− mast cells, along with an increase in reactive oxygen species, known to regulate Shp-1. Reduced Shp-1 activity concomitant with sustained Kit signaling resulted in greater proliferation following Kit engagement, and increased mediator and cytokine release when Aldh2−/− mast cells were co-stimulated via Kit and FcεRI. However, FcεRI-mediated signaling and responses were unaffected. Therefore, our findings reveal a functional role for mast cell intrinsic Aldh2 in the control of Kit activation and Kit-mediated responses, which may lead to a better understanding of mast cell reactivity in conditions related to ALDH2 polymorphisms.
Prevalence, Risk Factors and Course of Osteoporosis in Patients with Crohn’s Disease at a Tertiary Referral Center
Authors: Hoffmann Peter , Krisam Johannes , Kasperk Christian , Gauss Annika
Published: December 2019

Background: Patients with Crohn’s disease are at increased risk for fractures due to low bone mineral density (BMD). Real-world data are necessary to optimize surveillance and treatment strategies. Methods: Patients with Crohn’s disease who underwent at least one dual-energy X-ray absorptiometry (DXA) scans were recruited. The primary study endpoints were (1) prevalence of osteoporosis, and (2) factors influencing changes of BMD. To identify potential risk factors for reduced BMD, Mann–Whitney U-test was used for ordinal and continuous variables and x²-tests for categorical variables. Results with p < 0.05 were included in a multivariable analysis. To identify potential factors influencing changes in BMD, a generalized linear mixed model was applied. Results: 39.9% of the patients were diagnosed with normal BMD, 40.2% with osteopenia, and 19.8% with osteoporosis. The main risk factors for osteoporosis were low body mass index (BMI), previous bowel resections and male sex. The main risk factors for reduced BMD during further along the disease course were steroid use, history of immunomodulator treatment, female sex and decreased BMI. Conclusion: Low BMI, previous bowel resections and male sex were the main risk factors for the development of osteoporosis. Steroid use reduced BMD even under anti-inflammatory therapy, underlining that they should be used with great care in that patient group.
Tribological Properties of Alkyldiphenylethers in Boundary Lubrication
Authors: Lu Renguo , Morimoto Masaya , Tani Hiroshi , Tagawa Norio , Koganezawa Shinji
Published: December 2019

Lubricants that are used in miniaturized moving mechanical components generally serve under severe conditions, such as high temperature, high speed, and high load. Although alkyldiphenylethers (ADEs) are used as base oils for high-temperature greases, their tribological properties remain unclear. This study investigated the influence of the alkyl chains on the tribological properties of ADEs. Longer and more attached alkyl chains decreased the friction coefficient of ADEs under both reciprocating and continuous sliding conditions. Wear was found to be independent of the alkyl chain under reciprocating sliding conditions due to abrasion being caused by debris that was not readily removed. ADEs showed good anti-wear properties when used as either a lubricant or an additive under continuous sliding conditions. Much smoother surfaces in the friction track were observed in comparison to poly-α-olefin. Fourier-Transform infrared spectroscopy analysis suggested that the ether groups were attracted to the worn steel surface, phenyl groups became consequently perpendicular, and the attached alkyl chains repelled other substances and prevented further wear of the surface. Moreover, ADEs showed a high adaptability with traditional additives.
Access to d- and l-Psicose Derivatives via Hydroxy Methylation of Ribono Lactone
Authors: Imrich Michael R. , Ziegler Thomas
Published: December 2019

2,3,5-Tri-O-benzyl- and 2,3,5-tri-O-methyl-d-ribono-γ-lactone were converted with (methoxyethoxymethoxy)methyl and benzyloxy tributylstannane into the corresponding protected d-psicoses as mixtures of anomers in 31%–72% yield. Treatment of 2,3,5-tri-O-methyl-l-ribono-γ-lactone with benzyloxy tributylstannane afforded the corresponding l-psicose derivative as an anomeric mixture in 72% yield. Both methylated psicoses were further converted into 1,2-O-isopropylidene-3,4,6-tri-O-methyl-d- and l-psicofuranosides, the respective α- and β-anomers of which could be separated and characterized.
Damage Mechanism of Cu6Sn5 Intermetallics Due to Cyclic Polymorphic Transitions
Authors: Zhang Zhihao , Wei Cunwei , Cao Huijun , Zhang Ye
Published: December 2019

The formation of high-melting-point Cu6Sn5 interconnections is crucial to overcome the collapse of Sn-based micro-bumps and to produce reliable intermetallic interconnections in three-dimensional (3D) packages. However, because of multiple reflows in 3D package manufacturing, Cu6Sn5 interconnections will experience cyclic polymorphic transitions in the solid state. The repeated and abrupt changes in the Cu6Sn5 lattice due to the cyclic polymorphic transitions can cause extreme strain oscillations, producing damage at the surface and in the interior of the Cu6Sn5 matrix. Moreover, because of the polymorphic transition-induced grain splitting and superstructure phase formation, the reliability of Cu6Sn5 interconnections will thus face great challenges in 3D packages. In addition, the Cu6Sn5 polymorphic transition is structure-dependent, and the η′↔η polymorphic transition will occur at the surface while the η′↔ηs↔η polymorphic transition will occur in the deep matrix. This study can provide in-depth understanding of the structural evolution and damage mechanism of Cu6Sn5 interconnections in real 3D package manufacturing.
Diffusion Mechanism of Rejuvenator and Its Effects on the Physical and Rheological Performance of Aged Asphalt Binder
Authors: Wang Fusong , Zhang Lei , Yan Boxiang , Kong Dezhi , Li Yuanyuan , Wu Shaopeng
Published: December 2019

Using rejuvenator to improve the asphalt pavement service state has become an increasing concern in recent years. This study mainly focuses on the diffusion analysis between rejuvenator and aged asphalt, and further rheological influences by addition of rejuvenators. First, two rejuvenators, oil rejuvenator (OR) and penetrative rejuvenator (PR), were prepared in the laboratory. Afterward, the diffused performance of rejuvenators was investigated by viscosity, contact angle, and three self-designed feasible test indexes, which were sinking time test, softening rate test, and gravitational collapsing test. Beside the comparison in physical properties tests of aged and rejuvenated asphalt, their rheological performances were also evaluated by dynamic shear rheometer (DSR) and bending beam rheometer (BBR) respectively. The results indicated that three proposed indexes can comparatively analyze the diffusion rate of different rejuvenators on aged asphalt effectively. Furthermore, all adopted test indexes signaled that PR has a faster diffusion rate and better penetrative efficiency. Comparatively, exceeding rejuvenator dosage would increase the rutting possibility. Nevertheless, 2.5 wt% addition dosage for both OR and PR into aged asphalt can promote appropriate improvement in physical properties and crack resistance. The study would supply a significant reference for penetrative rejuvenator preparation and its diffusing evaluation.
A Parameter Study for 3D-Printing Organized Nanofibrous Collagen Scaffolds Using Direct-Write Electrospinning
Authors: Alexander Frank A. , Johnson Lee , Williams Krystaufeux , Packer Kyle
Published: December 2019

Collagen-based scaffolds are gaining more prominence in the field of tissue engineering. However, readily available collagen scaffolds either lack the rigid structure (hydrogels) and/or the organization (biopapers) seen in many organ tissues, such as the cornea and meniscus. Direct-write electrospinning is a promising potential additive manufacturing technique for constructing highly ordered fibrous scaffolds for tissue engineering and foundational studies in cellular behavior, but requires specific process parameters (voltage, relative humidity, solvent) in order to produce organized structures depending on the polymer chosen. To date, no work has been done to optimize direct-write electrospinning parameters for use with pure collagen. In this work, a custom electrospinning 3D printer was constructed to derive optimal direct write electrospinning parameters (voltage, relative humidity and acetic acid concentrations) for pure collagen. A LabVIEW program was built to automate control of the print stage. Relative humidity and electrospinning current were monitored in real-time to determine the impact on fiber morphology. Fiber orientation was analyzed via a newly defined parameter (spin quality ratio (SQR)). Finally, tensile tests were performed on electrospun fibrous mats as a proof of concept.
Effect of Residual Stress on Thermal Deformation Behavior
Authors: Sasaki Tomohiro , Yoshida Sanichiro , Ogawa Tadashi , Shitaka Jun , McGibboney Conor
Published: December 2019

This paper discusses a non-destructive measurement technique of residual stress through optical visualization. The least amount of deformation possible is applied to steel plates by heating the specimens +10 °C from room temperature for initial calibration, and the thermal expansion behavior is visualized with an electronic speckle pattern interferometer sensitive to two dimensional in-plane displacement. Displacement distribution with the thermal deformation and coefficient of thermal expansion are obtained through interferometric fringe analysis. The results suggest the change in the thermal deformation behavior is affected by the external stress initially applied to the steel specimen. Additionally, dissimilar joints of steel and cemented carbide plates are prepared by butt-brazing. The residual stress is estimated based on the stress dependence of thermal expansion coefficient.
Modulation Equation for the Stochastic Swift–Hohenberg Equation with Cubic and Quintic Nonlinearities on the Real Line
Authors: Mohammed Wael W.
Published: December 2019

The purpose of this paper is to rigorously derive the cubic–quintic Ginzburg–Landau equation as a modulation equation for the stochastic Swift–Hohenberg equation with cubic–quintic nonlinearity on an unbounded domain near a change of stability, where a band of dominant pattern is changing stability. Also, we show the influence of degenerate additive noise on the stabilization of the modulation equation.
Periodic Solutions for a Four-Dimensional Coupled Polynomial System with N-Degree Homogeneous Nonlinearities
Authors: Tian Yuanyuan , Li Jing
Published: December 2019

This paper studies the periodic solutions of a four-dimensional coupled polynomial system with N-degree homogeneous nonlinearities of which the unperturbed linear system has a center singular point in generalization resonance 1 : n at the origin. Considering arbitrary positive integers n and N with n ≤ N and N ≥ 2 , the new explicit expression of displacement function for the four-dimensional system is detected by introducing the technique on power trigonometric integrals. Then some precise and detailed results in comparison with the existing works, including the existence condition, the exact number, and the parameter control conditions of periodic solutions, are obtained, which can provide a new theoretical description and mechanism explanation for the phenomena of emergence and disappearance of periodic solutions. Results obtained in this paper improve certain existing results under some parameter conditions and can be extensively used in engineering applications. To verify the applicability and availability of the new theoretical results, as an application, the periodic solutions of a circular mesh antenna model are obtained by theoretical method and numerical simulations.
The Anti-Campylobacter Activity and Mechanisms of Pinocembrin Action
Authors: Klančnik Anja , Šimunović Katarina , Kovac Jasna , Sahin Orhan , Wu Zuowei , Vučković Darinka , Abram Maja , Zhang Qijing , Smole Možina Sonja
Published: December 2019

We investigated the anti-Campylobacter activity of pinocembrin and its mechanism of action, as well as Campylobacter responses to pinocembrin treatment at the genetic and phenotypic levels, using C. jejuni NCTC 11168 and a multidrug efflux system repressor mutant (11168ΔcmeR). At its minimal inhibitory concentration, pinocembrin significantly increased cell membrane permeability of Campylobacter. Interestingly, at sub-inhibitory concentrations, pinocembrin did not significantly alter membrane functionality and it increased bacterial fitness. Treatment with pinocembrin evoked decreased expression of ribosomal proteins and down-regulation of several NADH dehydrogenase I chain subunits and proteins involved in iron uptake. This suggests altered protein production and redox cycle and iron metabolism. Interestingly, the chelation of Fe ions during the treatment with pinocembrin increased C. jejuni survival, although there was no increase in the formation of reactive oxygen species. Pre-treatment of C. jejuni with sub-inhibitory concentrations of pinocembrin for 2 h resulted in a 1 log decrease in C. jejuni colony forming units in mice liver at 8 days post-infection, compared to untreated C. jejuni. These findings suggest that pinocembrin modulates the metabolic activity of C. jejuni and that pre-treatment of C. jejuni with pinocembrin influences its virulence potential in mice. This anti-Campylobacter potential of pinocembrin warrants further investigation.
Lithium Occurrences in Brines from Two German Salt Deposits (Upper Permian) and First Results of Leaching Experiments
Authors: Mertineit Michael , Schramm Michael
Published: December 2019

Lithium occurrences were detected in Upper Permian (Zechstein) salt rocks and saline solutions of the Gorleben and Morsleben salt structures, northern Germany. The brine occurrences were mainly connected to anhydrite rock-bearing formations and to lithological boundaries. Most of these brines display a high Mg content and were accordingly interpreted as intrasalinar solutions, which developed during sedimentation, diagenesis, and the subsequent rock–fluid interaction. These Mg-rich brines frequently show high Li concentrations. One of the assumptions made, is that Li was leached from phyllosilicates, since no natural Li-bearing salt minerals are known to date. To improve the understanding of the origin of Li in the brines, leaching experiments were performed on the Li-bearing phyllosilicate Lepidolite. Lepidolite with a Li content of 2.42 wt. % served as an analogue material, which was exposed to 18 saline solutions of different composition for a period of three years. The most pronounced leaching effect (53.36 µg Li/g in the brine) was observed during the interaction with a 0.03 mol/kg H2O MgCl2 solution, the second most pronounced by modern seawater interaction. The experiments show that the amount of Li leached from the lepidolite is dependent on brine composition.
Geochemical Features and Geological Processes Timescale of the Achaean TTG Complexes of the Ingozero Massif and the Pechenga Frame (NE Baltic Shield)
Authors: Nitkina Elena , Kozlov Nikolay , Kozlova Natalia , Kaulina Tatiana
Published: December 2019

This article provides a geological review and results of the structural, metamorphic, and geochronological studies of the Pechenga frame outcrops located in the NW part of the Central-Kola terrain and the Ingozero massif outcrops situated in the northeastern part of the Belomorian mobile belt of the Kola Region (NW Baltic Shield). As a result of the work, the deformation scales and ages of the geological processes at the Neo-Archaean–Paleoproterozoic stage of the area’s development were compiled, and the reference rocks were dated. The petrochemical and geochemical characteristics of the Ingozero rocks are similar to those of tonalite–trondhjemite–granodiorite (TTG) complexes established on other Archaean shields. The isotope U–Pb dating of individual zircon grains from the biotite gneisses provided the oldest age for magmatic protolith of the Ingozero gneisses, which is 3149 ± 46 Ma. Sm–Nd model ages showed that the gneisses protolite initial melt formed at 3.1–2.8 Ga. Ages of metamorphic processes were determined by using isotope U–Pb dating ID TIMS (isotope dilution thermal ionization mass spectrometry): Biotite gneisses—2697 ± 9 Ma; amphibole–biotite gneisses—2725 ± 2 Ma and 2667 ± 7 Ma; and biotite–amphibole gneisses 2727 ± 5 Ma. Ages of granitoids, which cut the deformed gneisses, are 2615 ± 8 Ma and 2549 ± 31 Ma for plagiogranites and pegmatoid veins in gneisses, respectively. The following age sequence of geological processes was established by using U–Pb zircon dating: 2.8 Ga—The time of the garnet–biotite gneiss metamorphism; 2722 ± 9 Ma—The granodiorite crystallization time; 2636 ± 41 Ma—The aplite emplacement age and 2620 ± 16 Ma—The age of pegmatites origin, which marked final stages of the Archaean evolution; 2587 ± 5 Ma—The age of gabbros emplacement and 2507 ± 7 Ma—The age of gabbros metamorphism; 2522–2503 Ma—The origin time of the iron quartzite interpreted as the age of gabbros and biotite gneiss metamorphism.
The Impact of the Ionic Cross-Linking Mode on the Physical and In Vitro Dexamethasone Release Properties of Chitosan/Hydroxyapatite Beads
Authors: L. Dantas Maria Jucélia , F. dos Santos Bárbara Fernanda , A. Tavares Albaniza , Maciel Matheus A. , Lucena Breno de Medeiros , L. Fook Marcus Vinícius , de L. Silva Suédina Maria
Published: December 2019

In this study, the effect of the ionic cross-linking mode on the ability to control physical properties and in vitro release behavior of the dexamethasone (DEX) drug from chitosan (CS) and chitosan/hydroxyapatite (CS/HA) beads was investigated. CS solutions without and with HA and DEX were dripped into two coagulation solutions, prepared with a non-toxic ionic crosslinker (sodium tripolyphosphate, TPP) and distilled water, one at pH = 9.0 and other at pH = 6.0. Optical microscopy (OM) and scanning electron microscopy (SEM) results showed changes on the surface topology of the beads, with a reduction of roughness for beads prepared at pH = 6.0 and an increase for the one prepared at pH = 9.0. The diameter and sphericity of the beads prepared at pH = 6.0 proved more uniform and had a larger pore size with a good interconnectivity framework. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) suggested a higher crosslinking degree for beads prepared at pH = 6.0, corroborated by X-ray diffraction profiles (XRD) analysis that indicated a decrease in the crystalline structure for such beads. In in vitro drug release data, all beads presented a sustained release during the studied period (24 h). The drug release rate was affected by the pH of the coagulation solution used in the preparation of the beads. The in vitro kinetics of the release process was of the Peppas–Sahlin model, controlled by both diffusion and relaxation of polymer chains or swelling (anomalous transport mechanism). Our results suggest that DEX-loaded CS/HA beads, crosslinked in TPP coagulation solution at pH = 9.0, led to a decrease in the DEX release rate and prolonged the release period. Thus, this composition might have prospective as a functional material for bone and cartilage tissue engineering.
Taurochenodeoxycholic Acid Inhibited AP-1 Activation via Stimulating Glucocorticoid Receptor
Authors: Li Lei , Liu Chang , Mao Wei , Tumen Bayaer , Li Peifeng
Published: December 2019

Taurochenodeoxycholic acid (TCDCA) as a primary bioactive substance of animal bile has been shown to exert good anti-inflammatory and immunomodulatory functions in adjuvant arthritis in rats. The anti-inflammatory and immunomodulatory properties of TCDCA have exhibited interesting similarities with the effects of glucocorticoids (GCs). To investigate the potential mechanisms of TCDCA in anti-inflammation and immunomodulation, we used a luciferase reporter assay to evaluate the activation of the glucocorticoid receptor (GR) stimulated by TCDCA. Our results showed that GR was activated by TCDCA in a concentration-dependent manner. Moreover, the elevated expressions of c-Fos and phosphorylated c-Jun induced by interleukin-1β (IL-1β) were reversed by TCDCA. The inhibition of TCDCA on the transactivation of activator protein-1 (AP-1) was observed as well. However, the suppression of TCDCA on the phosphorylation of c-Jun was blocked incompletely by GR inhibitor RU486. These results have indicated that the anti-inflammatory and immunomodulatory functions of TCDCA involve multiple pathways, with contributions from GR and its related AP-1 signaling pathway.
Biological Role of Gellan Gum in Improving Scaffold Drug Delivery, Cell Adhesion Properties for Tissue Engineering Applications
Authors: Muthukumar Thangavelu , Song Jeong Eun , Khang Gilson
Published: December 2019

Over the past few decades, gellan gum (GG) has attracted substantial research interest in several fields including biomedical and clinical applications. The GG has highly versatile properties like easy bio-fabrication, tunable mechanical, cell adhesion, biocompatibility, biodegradability, drug delivery, and is easy to functionalize. These properties have put forth GG as a promising material in tissue engineering and regenerative medicine fields. Nevertheless, GG alone has poor mechanical strength, stability, and a high gelling temperature in physiological conditions. However, GG physiochemical properties can be enhanced by blending them with other polymers like chitosan, agar, sodium alginate, starch, cellulose, pullulan, polyvinyl chloride, xanthan gum, and other nanomaterials, like gold, silver, or composites. In this review article, we discuss the comprehensive overview and different strategies for the preparation of GG based biomaterial, hydrogels, and scaffolds for drug delivery, wound healing, antimicrobial activity, and cell adhesion. In addition, we have given special attention to tissue engineering applications of GG, which can be combined with another natural, synthetic polymers and nanoparticles, and other composites materials. Overall, this review article clearly presents a summary of the recent advances in research studies on GG for different biomedical applications.
A Machine Learning-Based Raman Spectroscopic Assay for the Identification of Burkholderia mallei and Related Species
Authors: Moawad Amira A. , Silge Anja , Bocklitz Thomas , Fischer Katja , Rösch Petra , Roesler Uwe , Elschner Mandy C. , Popp Jürgen , Neubauer Heinrich
Published: December 2019

Burkholderia (B.) mallei, the causative agent of glanders, and B. pseudomallei, the causative agent of melioidosis in humans and animals, are genetically closely related. The high infectious potential of both organisms, their serological cross-reactivity, and similar clinical symptoms in human and animals make the differentiation from each other and other Burkholderia species challenging. The increased resistance against many antibiotics implies the need for fast and robust identification methods. The use of Raman microspectroscopy in microbial diagnostic has the potential for rapid and reliable identification. Single bacterial cells are directly probed and a broad range of phenotypic information is recorded, which is subsequently analyzed by machine learning methods. Burkholderia were handled under biosafety level 1 (BSL 1) conditions after heat inactivation. The clusters of the spectral phenotypes and the diagnostic relevance of the Burkholderia spp. were considered for an advanced hierarchical machine learning approach. The strain panel for training involved 12 B. mallei, 13 B. pseudomallei and 11 other Burkholderia spp. type strains. The combination of top- and sub-level classifier identified the mallei-complex with high sensitivities (>95%). The reliable identification of unknown B. mallei and B. pseudomallei strains highlighted the robustness of the machine learning-based Raman spectroscopic assay.
Insecticidal Activity and Insecticidal Mechanism of Total Saponins from Camellia oleifera
Authors: Cui Chuanjian , Yang Yunqin , Zhao Tianyu , Zou Kangkang , Peng Chuanyi , Cai Huimei , Wan Xiaochun , Hou Ruyan
Published: December 2019

Chemical pesticides are commonly used during the cultivation of agricultural products to control pests and diseases. Excessive use of traditional pesticides can cause environmental and human health risks. There are ongoing searches for new plant-derived pesticides to reduce the use of chemical pesticides. In this study, tea saponin extracts of different purities were extracted from Camellia oleifera seeds using AB-8 macroporous resin and gradient elution with ethanol. The insecticidal effects of the tea saponin extracts were evaluated by contact toxicity tests and stomach toxicity tests using the lepidopteran pest of tea plantation, Ectropis obliqua. The total saponins extracted using 70% ethanol showed strong contact toxicity (LC50 = 8.459 mg/L) and stomach toxicity (LC50 = 22.395 mg/L). In-depth mechanistic studies demonstrated that tea saponins can disrupt the waxy layer of the epidermis, causing serious loss of water, and can penetrate the inside of the intestine of E. obliqua. After consumption of the tea saponins, the intestinal villi were shortened and the cavities of the intestinal wall were disrupted, which resulted in larval death. This study highlights the potential of tea saponins as a natural, plant-derived pesticide for the management of plant pests.
Evodiamine Eliminates Colon Cancer Stem Cells via Suppressing Notch and Wnt Signaling
Authors: Kim Hyejin , Yu Yeongji , Choi SeokGyeong , Lee Hani , Yu Jinsuh , Lee Jeong-Ho , Kim Woo-Young
Published: December 2019

Evodiamine, an alkaloid contained in traditional Asian herbal medicines that have been used for hundreds years, is interesting due to its cytotoxic effects against many cancers. We examined the effect of evodiamine on the cancer stem cell (CSC) population and the bulk cultured cancer cells (BCC) of colon cancers to examine the double targeting effect. We found that three colon cancer cell lines’ BCC and CSC are effectively targeted by evodiamine. Evodiamine was able to suppress BCC proliferation and induce apoptosis of the cells captured in G2/M phase, as previously reported. However, evodiamine did not cause the accumulation of CSCs at a certain stage of the cell cycle, resulting in the elimination of stemness through an unknown mechanism. By analyzing the expression of 84 genes related to CSCs in two colon cancer cell lines’ CSC, as well as performing further informatics analyses, and quantitative RT-PCR analyses of 24 CSC genes, we found that evodiamine suppressed the expression of the genes that control key signaling pathways of CSC, namely, WNT and NOTCH signaling, to lead CSC elimination. These results suggest that evodiamine should be further developed for targeting both BCCs and CSCs in colon cancers.
Questiomycins, Algicidal Compounds Produced by the Marine Bacterium Alteromonas sp. D and Their Production Cue
Authors: Umetsu Saki , Kanda Mamoru , Imai Ichiro , Sakai Ryuichi , Fujita Masaki J.
Published: December 2019

Questiomycin A (1) along with three new compounds, questiomycins C–E (2–4), were isolated from culture of Alteromonas sp. D, an algicidal marine bacterium, guided by algal lethality assay using the raphidophyte, Chattonella antiqua, one of the causative organisms of harmful algal bloom. The structures of 1–4 were assigned on the basis of their spectrometric and spectroscopic data. Compounds 1 to 4 exhibited algicidal activity against C. antiqua with LC50 values ranging from 0.18 to 6.37 M. Co-cultivation experiment revealed that 1 was produced only when the microalgae and the bacterium are in close contact, suggesting that some interactions between them trigger the biosynthesis of questiomycins. These results suggested that the algicidal bacteria such as Alteromonas sp. D can control microalgae chemically in marine ecosystem.
Metal Organic Frameworks as Desulfurization Adsorbents of DBT and 4,6-DMDBT from Fuels
Authors: Kampouraki Zoi-Christina , Giannakoudakis Dimitrios A. , Nair Vaishakh , Hosseini-Bandegharaei Ahmad , Colmenares Juan Carlos , Deliyanni Eleni A.
Published: December 2019

Ultradeep desulfurization of fuels is a method of enormous demand due to the generation of harmful compounds during the burning of sulfur-containing fuels, which are a major source of environmental pollution. Among the various desulfurization methods in application, adsorptive desulfurization (ADS) has low energy demand and is feasible to be employed at ambient conditions without the addition of chemicals. The most crucial factor for ADS application is the selection of the adsorbent, and, currently, a new family of porous materials, metal organic frameworks (MOFs), has proved to be very effective towards this direction. In the current review, applications of MOFs and their functionalized composites for ADS are presented and discussed, as well as the main desulfurization mechanisms reported for the removal of thiophenic compounds by various frameworks. Prospective methods regarding the further improvement of MOF’s desulfurization capability are also suggested.
The Acute and Chronic Cognitive and Cerebral Blood-Flow Effects of Nepalese Pepper (Zanthoxylum armatum DC.) Extract—A Randomized, Double-Blind, Placebo-Controlled Study in Healthy Humans
Authors: Kennedy David , Wightman Emma , Khan Julie , Grothe Torsten , Jackson Philippa
Published: December 2019

Background: Zanthoxylum armatum DC. (ZA) is a traditional Asian culinary spice and medicinal compound, which is rich in monoterpenes and hydroxy α-sanshool. Mechanistic interactions with the monoamine, cholinergic and cannabinoid neurotransmission systems, as well as transient receptor potential (TRP) and potassium ion channels, may predispose ZA to modulate human brain function. Objectives: To investigate the effects of a single dose and 56-days supplementation with a lipid extract of ZA on cognitive function, mood and cerebral blood-flow (CBF) parameters in the pre-frontal cortex during cognitive task performance. Design: Double-blind, randomized, parallel groups study with N = 82 healthy males and females between the ages of 30 and 55 years. Assessments were undertaken pre-dose and at 1, 3 and 5 h post-dose on the first (Day 1) and last (Day 56) days of supplementation. Results: A single dose of ZA (Day 1) resulted in acute improvements on a ‘Speed of Attention’ factor and the Rapid Visual Information Processing (RVIP) task, in comparison to placebo. However, following ZA participants were less accurate on the name-to-face recall task. After 56 days of ZA consumption (Day 56), speed was enhanced on a global ‘Speed of Performance’ measure, comprising data from all of the timed tasks in the computerized battery. Participants also completed more correct Serial 3s Subtractions at the 3 h assessment and were less mentally fatigued throughout the day than participants consuming placebo. These effects were complemented on both Day 1 and Day 56 by modulation of CBF parameters, as assessed by Near Infrared Spectroscopy (NIRS). The primary finding here was a reduced hemodynamic response during the RVIP task. Conclusion: ZA improves aspects of cognitive performance, in particular the speed of performing tasks, in healthy humans and results in concomitant reductions in hemodynamic responses in the frontal cortex during task performance. The findings suggest an increase in neural efficiency following ZA.
Preparation of Squalene Oil-Based Emulsion Adjuvants Employing a Self-Emulsifying Drug Delivery System and Assessment of Mycoplasma hyopneumoniae-Specific Antibody Titers in BALB/c Mice
Authors: Bastola Rakesh , Seo Jo-Eun , Keum Taekwang , Noh Gyubin , Choi Jae Woong , Shin Jong Il , Kim Ju Hun , Lee Sangkil
Published: December 2019

In this study, a self-emulsifying drug delivery system (SEDDS) was employed to prepare novel squalene oil-based emulsion adjuvants. Deionized water, 0.01% and 0.02% (w/v) carbomer solutions of C-971P NF and C-940 grades were used to prepare emulsions containing 3%, 5% and 10% of squalene oil. Altogether 15 candidate emulsions were prepared and used as adjuvants for the delivery of a combination vaccine containing a porcine circovirus type 2 (PCV2) antigen and inactivated Mycoplasma hyopneumoniae (J101 strain) antigen. Most of the emulsions showed droplet sizes in the submicron range and maintained zeta potential values between −40 mV to 0 mV for six months, indicating good physical stability as a vaccine adjuvant. Emulsion-based candidate adjuvants prepared with SEDDS technology stimulated IgG, IgG1 and IgG2a like a currently commercially available adjuvant, Montanide ISATM 201, and they were safe and their Mycoplasma hyopneumoniae-specific antibody titers were considered as comparable with that of Montanide ISATM 201.
Fault Diagnosis of the Blocking Diesel Particulate Filter Based on Spectral Analysis
Authors: Liu Shuang-xi , Lü Ming
Published: December 2019

Diesel particulate filter is one of the most effective after-treatment techniques to reduce Particulate Matters (PM) emissions from a diesel engine, but the blocking Diesel Particulate Filter (DPF) will seriously affect the engine performance, so it is necessary to study the fault diagnosis of blocking DPF. In this paper, a simulation model of an R425DOHC diesel engine with wall-flow ceramic DPF was established, and then the model was verified with experimental data. On this basis, the fault diagnosis of the blocking DPF was studied by using spectral analysis on instantaneous exhaust pressure. The results showed that both the pre-DPF mean exhaust pressure and the characteristic frequency amplitude of instantaneous exhaust pressure can be used as characteristic parameters of monitoring the blockage fault of DPF, but it is difficult to monitor DPF blockage directly by instantaneous exhaust pressure. In terms of sensitivity, the characteristic frequency amplitude of instantaneous exhaust pressure is more suitable as a characteristic parameter to monitor DPF blockage than mean exhaust pressure. This work can lay an important theoretical foundation for the on-board diagnosis of DPF.
Ab Initio Molecular Dynamics Simulations to Interpret the Molecular Fragmentation Induced in Deoxyribose by Synchrotron Soft X-Rays
Authors: Hervé du Penhoat Marie-Anne , Hamila Anis , Gaigeot Marie-Pierre , Vuilleumier Rodolphe , Fujii Kentaro , Yokoya Akinari , Politis Marie-Françoise
Published: December 2019

It has been suggested that core ionization in DNA atoms could induce complex, irreparable damage. Synchrotron soft X-rays have been used to probe the damage induced by such events in thin films of DNA components. In a complementary approach, we investigate the fragmentation dynamics following a carbon or oxygen K-shell ionization in 2-deoxy-D-ribose (DR), a major component in the DNA chain. Core ionization of the sugars hydration layer is also studied. To that aim, we use state-of-the-art ab initio Density Functional Theory-based Molecular Dynamics (MD) simulations. The ultrafast dissociation dynamics of the core ionized molecule, prior Auger decay, is modeled for about 10 fs. We show that the core-ionization of oxygen atoms within DR or its hydration layer may induce proton transfers towards nearby molecules, before Auger decay. In a second step, we model an Auger effect occurring either at the beginning or at the end of the core–hole dynamics. Two electrons are removed from the deepest valence molecular orbitals localized on the initially core-ionized oxygen atom (O*), and this electronic state is propagated by means of Ehrenfest MD. We show an ultrafast dissociation of the DR2+ molecule C-O* bonds, which, in most cases, seems independent of the time at which Auger decay occurs.
A Likelihood Approach to Bornhuetter–Ferguson Analysis
Authors: Elpidorou Valandis , Margraf Carolin , Martínez-Miranda María Dolores , Nielsen Bent
Published: December 2019

A new Bornhuetter–Ferguson method is suggested herein. This is a variant of the traditional chain ladder method. The actuary can adjust the relative ultimates using externally estimated relative ultimates. These correspond to linear constraints on the Poisson likelihood underpinning the chain ladder method. Adjusted cash flow estimates were obtained as constrained maximum likelihood estimates. The statistical derivation of the new method is provided in the generalised linear model framework. A related approach in the literature, combining unconstrained and constrained maximum likelihood estimates, is presented in the same framework and compared theoretically. A data illustration is described using a motor portfolio from a Greek insurer.
Assessing the Potential of Geostationary Satellites for Aerosol Remote Sensing Based on Critical Surface Albedo
Authors: Ceamanos Xavier , Moparthy Suman , Carrer Dominique , Seidel Felix C.
Published: December 2019

Geostationary satellites are increasingly used for the detection and tracking of atmospheric aerosols and, in particular, of the aerosol optical depth (AOD). The main advantage of these spaceborne platforms in comparison with polar orbiting satellites is their capability to observe the same region of the Earth several times per day with varying geometry. This provides a wealth of information that makes aerosol remote sensing possible when combined with the multi-spectral capabilities of the on-board imagers. Nonetheless, the suitability of geostationary observations for AOD retrieval may vary significantly depending on their spatial, spectral, and temporal characteristics. In this work, the potential of geostationary satellites was assessed based on the concept of critical surface albedo (CSA). CSA is linked to the sensitivity of each spaceborne observation to the aerosol signal, as it is defined as the value of surface albedo for which a varying AOD does not alter the satellite measurement. In this study, the sensitivity to aerosols was determined by estimating the difference between the surface albedo of the observed surface and the corresponding CSA (referred to as dCSA). The values of dCSA were calculated for one year of observations from the Meteosat Second Generation (MSG) spacecraft, based on radiative transfer simulations and information on the satellite acquisition geometry and the properties of the observed surface and aerosols. Different spectral channels from MSG and the future Meteosat Third Generation-Imager were used to study their distinct capabilities for aerosol remote sensing. Results highlight the significant but varying potential of geostationary observations across the observed Earth disk and for different time scales (i.e., diurnal, seasonal, and yearly). For example, the capability of sensing multiples times during the day is revealed to be a notable strength. Indeed, the value of dCSA often fluctuates significantly for a given day, which makes some instants of time more suitable for aerosol retrieval than others. This study determines these instants of time as well as the seasons and the sensing wavelengths that increase the chances for aerosol remote sensing thanks to the variations of dCSA. The outcomes of this work can be used for the development and refinement of AOD retrieval algorithms through the use of the concept of CSA. Furthermore, results can be extrapolated to other present-day geostationary satellites such as Himawari-8/9 and GOES-16/17.
Temperature and Emissivity Smoothing Separation with Nonlinear Relation of Brightness Temperature and Emissivity for Thermal Infrared Sensors
Authors: Miao Xinyuan , Zhang Ye , Zhang Junping , Zhou Xinyu
Published: December 2019

Aiming at low spectral contrast materials, the Optimized Smoothing for Temperature Emissivity Separation (OSTES) method was developed to improve the Temperature and Emissivity Separation (TES) algorithm based on the linear relationship between brightness temperature and emissivity features, but there was little smoothing improvement for higher spectral contrast materials. In this paper, a new nonlinear-relationship based algorithm is presented, focusing on improving the performance of the OSTES method for materials with middle or high spectral contrast. This novel approach is a two-step procedure. Firstly, by introducing atmospheric impact factor, the nonlinear relationship is mathematically proved using first-order Taylor series approximation. Moreover, it is proven that nonlinear model has stronger universality than linear model. Secondly, a new method named Temperature and Emissivity Separation with Nonlinear Constraint (TESNC) is proposed based on the nonlinear model for smoothing temperature and emissivity retrieval. The key procedure of TESNC is the lowest emissivity smoothing estimation based on nonlinear model and retrieved by minimizing the reconstruction error of the Planck radiance. TESNC was tested on a series of synthetic data with different kinds of natural materials representing several multispectral and hyperspectral infrared sensors. It is shown that, especially for materials with higher spectral contrast, the proposed method is less sensitive to changes in atmospheric conditions and sample temperatures. Furthermore, the standard Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) products in different kind of atmospheric conditions were used for verifying the improvement. TESNC is more accurate and stable with the decrease of emissivity and changes of atmospheric conditions compared with TES, Adjusted Normalized Emissivity Method (ANEM), and OSTES methods.
Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review
Authors: Li Zhanhong , Mohamed Mona A. , Vinu Mohan A. M. , Zhu Zhigang , Sharma Vinay , Mishra Geetesh K. , Mishra Rupesh K.
Published: December 2019

Aptamers are synthetic bio-receptors of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) origin selected by the systematic evolution of ligands (SELEX) process that bind a broad range of target analytes with high affinity and specificity. So far, electrochemical biosensors have come up as a simple and sensitive method to utilize aptamers as a bio-recognition element. Numerous aptamer based sensors have been developed for clinical diagnostics, food, and environmental monitoring and several other applications are under development. Aptasensors are capable of extending the limits of current analytical techniques in clinical diagnostics, food, and environmental sample analysis. However, the potential applications of aptamer based electrochemical biosensors are unlimited; current applications are observed in the areas of food toxins, clinical biomarkers, and pesticide detection. This review attempts to enumerate the most representative examples of research progress in aptamer based electrochemical biosensing principles that have been developed in recent years. Additionally, this account will discuss various current developments on aptamer-based sensors toward heavy metal detection, for various cardiac biomarkers, antibiotics detection, and also on how the aptamers can be deployed to couple with antibody-based assays as a hybrid sensing platform. Aptamers can be used in various applications, however, this account will focus on the recent advancements made toward food, environmental, and clinical diagnostic application. This review paper compares various electrochemical aptamer based sensor detection strategies that have been applied so far and used as a state of the art. As illustrated in the literature, aptamers have been utilized extensively for environmental, cancer biomarker, biomedical application, and antibiotic detection and thus have been extensively discussed in this article.