bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Uneven Secondary Air Supply and Burner Tilt on Flow Pattern, Heat Transfer, and NOx Emissions in a 500 MWe Tangential-Firing Coal Boiler
Authors: Jo Hyunbin , Park Jongkeun , Kang Woosuk , Hong Junseok , Yoon Sungmin , Ra Howon , Ryu Changkook
Published: December 2021

Tangential-firing boilers develop large swirling fireballs by using pulverized coal and air from the corners of the burner zone. During operation, however, the boiler may experience an uneven air supply between corners; this deforms the fireball, raising various issues concerning performance and structural safety. This study investigated the characteristic boiler performance and the role of burner tilting in a 500 MWe boiler with secondary air (SA) in two corners that are up to 1.9 times larger than those in the other corners. Computational fluid dynamics simulations with advanced coal combustion sub-models were employed with the following two sets of cases: (i) six cases of actual operation to validate the modeling and (ii) sixteen cases for the parametric study of SA flow ratio and burner tilt between −15° and +26°. The results showed that the uneven SA supply deteriorated the boiler performance in various aspects and the burner tilt can be used to alleviate its impact. With a larger SA supply from the left wind box, the mass flow, heat absorption, and O2 concentration were larger in the right half of the heat exchanger sections owing to the rotating flow. The corresponding imbalance in the reaction stoichiometry increased the peak temperature entering the tube bundles by up to 60 °C and NO emissions by 6.7% as compared with normal operations. The wall heat absorption was up to 19% larger on the right and front walls. The high burner tilt of +26° helped alleviate the impact of uneven SA supply on the heat distribution and uniformity of the flow pattern and temperature, whereas a +15° burner tilt was the least favorable.
Selected Problems of Power MOSFETs Thermal Parameters Measurements
Authors: Górecki Krzysztof , Posobkiewicz Krzysztof
Published: December 2021

In the paper, selected problems that are related to the measurements of thermal parameters of power MOSFETs that are placed on a common heat sink are analysed. The application of the indirect electrical method, the contact method, and the optical method in measuring self and mutual transient thermal impedances of these transistors is presented. The circuits that are required to perform measurements are presented and described. The errors of measurements are assessed for each of the considered methods. In the case of the indirect electrical method, an additional influence of the selection of a thermo-sensitive parameter and the function approximating thermometric characteristics on the measurement error are taken into consideration. The measurement results of the thermal parameters of the investigated transistors that were obtained using the considered measurement methods in various supply conditions are presented and discussed.
Comparing 2D and 3D Solar Radiation Modeling in Urban Areas
Authors: Kolečanský Štefan , Hofierka Jaroslav , Bogľarský Jozef , Šupinský Jozef
Published: December 2021

The use of solar radiation in the urban environment is becoming increasingly important for the sustainable development of cities and human societies. Several factors influence the distribution of solar radiation in urban areas, including urban morphology and the physical properties of urban materials. Most of these factors can be modeled with a relatively high accuracy using 2D and 3D solar radiation models. In this paper, the r.sun and v.sun solar radiation models are used to calculate solar radiation for the city of Košice in Eastern Slovakia to assess the accuracy of both approaches for vertical surfaces frequently found in urban areas. The results were validated by pyranometer measurements. The results showed relatively good estimates by the 3D v.sun model and poor estimates by the 2D r.sun model. This can be attributed to an improper representation of vertical surfaces by a digital surface model, which has a strong impact on solar resource assessments. We found that 3D city models prepared in level of detail 2 (LoD2) are not always adequate in case of complex buildings with morphological structures, such as terraces. These cast shadows on facades especially when solar altitude is high and, thus, assessments, even by a 3D model, are inaccurate.
Comparative Multicriteria Analysis Methods for Ranking Sites for Solar Farm Deployment: A Case Study in Greece
Authors: Vagiona Dimitra G.
Published: December 2021

This study investigated the prioritization and ranking problem of the appropriate locations at which to deploy solar photovoltaic (PV) farms. Although different Multicriteria Decision Making (MCDM) methods can be found in the literature to address this problem, a comparative analysis of those methods is missing. The aim of this study is to compare four different MCDM approaches to evaluate and rank suitable areas for the deployment of solar PV farms, with the island of Rhodes (Greece) being used as an example. Feasible areas for the location of such facilities were identified with the use of Geographical Information Systems (GIS), by applying certain exclusion criteria found either in the national legislative framework or in the international literature. Data were obtained from Greek open geospatial data. The feasible sites were evaluated and ranked using four different MCDM methods: the Analytical Hierarchy Process (AHP), the Technique for Order Preference by Similarity to Ideal Solution (TOPSIS), the VIKOR (VIseKriterijumska Optimizacija I Kompromisno Resenje), and the PROMETHEE II (Preference Ranking Organization METHod for Enrichment of Evaluations) method. The best alternative rated according to three TOPSIS, VIKOR and PROMETHEE is site (S2). The second-best alternative in the above three methods is site (S1), while the worst is site (S3). The best alternative rated according to AHP (S4) is in sixth position according to TOPSIS and in fifth position VIKOR and PROMETHEE. The comparison demonstrated that different MCDM techniques may generate different ranks. The simultaneous use of several MCDM methods in energy siting problems is considered advantageous as it can help decision makers to select the most sustainable sites, avoiding the disadvantages and availing the advantages of each method.
Structural Stability of Urban Trees Using Visual and Instrumental Techniques: A Review
Authors: Linhares Camila S. F. , Gonçalves Raquel , Martins Luis M. , Knapic Sofia
Published: December 2021

This review focuses on tree health assessment in urban forest, specifically on the methodologies commonly used to detect levels, dimensions, and location of wood deterioration. The acknowledged benefits to the urban forestry area from the application of assessment techniques are also addressed. A summary is presented of the different methodologies, such as visual analyses, acoustic tomography, and digital wood inspection drill, with the underlined importance of the biodeterioration of wood by fungi and termites.
A GMRT Narrowband vs. Wideband Analysis of the ACT−CL J0034.4+0225 Field Selected from the ACTPol Cluster Sample
Authors: Sikhosana Sinenhlanhla P. , Knowles Kenda , Ishwara-Chandra C. H. , Hilton Matt , Moodley Kavilan , Gupta Neeraj
Published: December 2021

Low frequency radio observations of galaxy clusters are a useful probe of the non-thermal intracluster medium (ICM), through observations of diffuse radio emission such as radio halos and relics. Current formation theories cannot fully account for some of the observed properties of this emission. In this study, we focus on the development of interferometric techniques for extracting extended, faint diffuse emissions in the presence of bright, compact sources in wide-field and broadband continuum imaging data. We aim to apply these techniques to the study of radio halos, relics and radio mini-halos using a uniformly selected and complete sample of galaxy clusters selected via the Sunyaev-Zel’dovich (SZ) effect by the Atacama Cosmology Telescope (ACT) project, and its polarimetric extension (ACTPol). We use the upgraded Giant Metrewave Radio Telescope (uGMRT) for targeted radio observations of a sample of 40 clusters. We present an overview of our sample, confirm the detection of a radio halo in ACT−CL J0034.4+0225, and compare the narrowband and wideband analysis results for this cluster. Due to the complexity of the ACT−CL J0034.4+0225 field, we use three pipelines to process the wideband data. We conclude that the experimental spam wideband pipeline produces the best results for this particular field. However, due to the severe artefacts in the field, further analysis is required to improve the image quality.
New Formulations Loading Caspofungin for Topical Therapy of Vulvovaginal Candidiasis
Authors: Pérez-González Noelia , Bozal-de Febrer Nuria , Calpena-Campmany Ana C. , Nardi-Ricart Anna , Rodríguez-Lagunas María J. , Morales-Molina José A. , Soriano-Ruiz José L. , Fernández-Campos Francisco , Clares-Naveros Beatriz
Published: December 2021

Vulvovaginal candidiasis (VVC) poses a significant problem worldwide affecting women from all strata of society. It is manifested as changes in vaginal discharge, irritation, itching and stinging sensation. Although most patients respond to topical treatment, there is still a need for increase the therapeutic arsenal due to resistances to anti-infective agents. The present study was designed to develop and characterize three hydrogels of chitosan (CTS), Poloxamer 407 (P407) and a combination of both containing 2% caspofungin (CSP) for the vaginal treatment of VVC. CTS was used by its mucoadhesive properties and P407 was used to exploit potential advantages related to increasing drug concentration in order to provide a local effect. The formulations were physically, mechanically and morphologically characterized. Drug release profile and ex vivo vaginal permeation studies were performed. Antifungal efficacy against different strains of Candida spp. was also evaluated. In addition, tolerance of formulations was studied by histological analysis. Results confirmed that CSP hydrogels could be proposed as promising candidates for the treatment of VVC.
Garnet Chemical Zoning Based Thermobarometry: Method Evaluation and Applications in the Menderes Massif, Western Turkey
Authors: Etzel Thomas M. , Catlos Elizabeth J.
Published: December 2021

The garnet chemical zoning method (GZM) is a reliable thermodynamic approach for forward modeling pressure-temperature (P-T) paths using observed garnet and bulk rock compositions. However, intracrystalline diffusion is known to compromise the integrity of GZM modeled garnet-growth P-T paths. For this reason, extracting reliable metamorphic estimates from garnet-bearing schists in the Central Menderes Massif (CMM), western Turkey, has been difficult. To evaluate the impact of diffusion on GZM, we simulate garnet growth and diffusion for an average metapelite using the program Theria_G. Modeled garnet compositions from four simulations are used to estimate P-T conditions and paths by GZM, which are compared against Theria_G specified P-T-t trajectories. Factors influencing results are heating/cooling rate, grain size, and peak T. At a maximum T of 610 °C, both undiffused and diffused garnet compositions returned estimates comparable to prescribed conditions regardless of heating/cooling rate. Diffused profiles from simulations reaching a maximum T of 670 °C also reproduced prescribed P-T paths if tectonism occurred at high heating/cooling rates (50 °C/my). From these insights and additional Theria_G simulation-derived observations for CMM garnets, we deduce that metamorphism in the region exceeded 650 °C and achieved a maximum burial P between 8–10 kbar prior to Cenozoic exhumation.
Quaternary Coastal Landscape Evolution and Sea-Level Rise: An Example from South-East Sicily
Authors: Distefano Salvatore , Gamberi Fabiano , Borzì Laura , Di Stefano Agata
Published: December 2021

Coastal depositional environments are the site of complex interactions between continental and marine processes. Barrier islands are highly dynamic coastal systems, typical of these transitional environments, and are affected by sea level changes and the accumulation of transgressive deposits with the landwards migration of the coast. The offshore of Marzamemi (Syracuse Province, Sicily), in the south-eastern portion of the Hyblean foreland, represents an excellent site for the study of transgressive deposits and their connection with the sea-level changes. The available dataset consisted of new high-resolution bathymetry (Multibeam), whose description and interpretation through a Digital Elevation Model (DEM) was integrated with a grid of eighteen seismic profiles (SPARKER). In the investigated bathymetric range, from about −5 m to −60 m, a sensibly different morphological setting between the northern and southern sectors was evident. Within the whole study area, three bathymetric contours (−45 m, −35 m and −20 m) were identified and assumed as the markers of the main locations of the paleo-coastlines during the recent changes in the sea level. Along the northern sector, three submerged barrier-lagoon systems developed on a calcarenite substratum, marking important steps of the Late Quaternary sea-level rise. They coexisted with numerous karst forms (poljes and dolines). In the southern sector the transgressive environmental evolution was significantly different and submerged lagoons did not form. Here the outcropping calcarenite substratum was affected by the development of paleo-rivers and karsts structures, a tract in common along with many Mediterranean carbonate coastal areas.
From Lithological Modelling to Groundwater Modelling: A Case Study in the Tiber River Alluvial Valley
Authors: Di Salvo Cristina , Mancini Marco , Moscatelli Massimiliano , Simionato Maurizio , Cavinato Gian Paolo , Dimasi Michele , Stigliano Francesco
Published: December 2021

This study presents the results of a research project financed by the Lazio Regional Government. The research focused on defining an integrated model of recent alluvial deposits in the Tiber River. To achieve this objective, geological boreholes were made to monitor the aquifer and in situ and laboratory tests were carried out. The data obtained were used to detail stratigraphic aspects and improve the comprehension of water circulation beneath the recent alluvial deposits of the Tiber River in the urban area of Rome, between the Ponte Milvio bridge and the Tiber Island. The stratigraphic intervals recognised in the boreholes were parameterised based on their litho-technical characteristics. The new data acquired, and integrated with existing data in the database of Institute of Environmental Geology and Geoengineering of the Italian National Research Council, made it possible to produce a three-dimensional model of the lithologies in the study area. The model of the subsoil, simplified for applied reasons, was described in hydrostratigraphic terms: three different lithotypes were subjected to piezometric levels monitoring. Finally, the research generated a numerical hydrological model in a steady state. In general, this study demonstrates how a numerical hydrogeological model calibrated by piezometric monitoring data can support the construction of a geological model, discarding or confirming certain hypotheses and suggesting other means of reconstructing sedimentary bodies.
Laboratory Measurements to Image Endobenthos and Bioturbation with a High-Frequency 3D Seismic Lander
Authors: Schulze Inken , Wilken Dennis , Zettler Michael L. , Gogina Mayya , Schönke Mischa , Feldens Peter
Published: December 2021

The presented 3D seismic system operates three transducers (130 kHz) from a stationary lander and allows non-destructive imaging of small-scale objects within the top decimeters of silty sediments, covering a surface area of 0.2 m2. In laboratory experiments, samples such as shells, stones, and gummy worms of varied sizes (down to approx. 1 cm diameter) could be located in the 3D seismic cube to a depth of more than 20 cm and differentiated by a reflected amplitude intensity and spatial orientation. In addition, simulated bioturbation structures could be imaged. In a practical application, the system allows to determine the abundance of endobenthos and its dynamic in muddy deposits in-situ and thus identify the intensity of local bioturbation.
Editorial of Special Issue “Quaternary Sedimentary Successions”
Authors: Sciuto Francesco
Published: December 2021

The Quaternary is the chronostratigraphic range spanning from 2.58 Ma to the present [...]
Vietnamese Version of the Geriatric Depression Scale (30 Items): Translation, Cross-Cultural Adaptation, and Validation
Authors: Nguyen Thong Van , Nguyen Kien Trung , Nguyen Phuong Minh , Nguyen Nghiem Minh , Ly Chi Lan , Nguyen Thang , Nguyen Minh Thi Tuyet , Le Hoang Minh , Nguyen Xuyen Thi Kim , Duong Nghi Huynh Phuong , Veith Richard C. , Nguyen Tuan Van
Published: December 2021

The proportion of geriatric depression recorded in Vietnam was 66.9%. Depression in older people is a risk factor for problems related to dementia, poor quality of life, and suicide. To have a good Vietnamese questionnaire for assessing geriatric depression, we conducted the study to translate and cross-culturally adapt the Geriatric Depression Scale—long-form with 30 items (GDS-30). The study has two steps. Step 1 is a translation of the GDS-30 scale. We followed the guideline by Beaton et al., (2000 & 2007). Firstly, two translators (informed and uninformed) translated the questionnaires. Secondly, the translations were synthesized. Thirdly, back translation was performed by two translators fluent in both Vietnamese and English but completely unknown of the original version of the scale and did not have medical expertise. Finally, seven experts reached a consensus on the pre-final Vietnamese version (GDS-30). Step 2 is a field test of the questionnaires on people 60 years or older. Then, we determined the internal consistency and test-retest reliability of the questionnaire in 55 Vietnamese inpatients in a geriatric department. Construct validity was determined by examining the relationship between depressive scores and patient characteristics. The Vietnamese version of GDS-30 was built with the agreement of all experts on the semantic, idiomatic, experiential, and conceptual equivalences between the original and pre-final Vietnamese versions of the GDS-30. The Cronbach’s alpha coefficient value was 0.928, indicating the items’ adequate internal consistency. Spearman’s correlation coefficient value of total scores between the first and second interviews showed medium correlation (0.479, p < 0.001), and the stability is acceptable. The GDS-30 scale reached the construct validity because the proportion of geriatric depression according to GDS-30 was significantly different between characteristics groups, such as gender, employment, level of education, economic status, and sleep disturbance. The Vietnamese version of the GDS-30 scale had high consistency, satisfactory reliability, and understanding and can be used as a screening tool for depression in elderly patients in primary healthcare centers. This is the first depression rating scale for the elderly in Vietnam to be translated and validated. Non-psychiatric health professionals or patients can quickly self-assess and screen for the illness.
The Role of Probiotics in Chronic Rhinosinusitis Treatment: An Update of the Current Literature
Authors: Bianco Maria Rita , Ralli Massimo , Modica Domenico Michele , Amata Marta , Poma Salvatore , Mattina Gianfranco , Allegra Eugenia
Published: December 2021

Chronic rhinosinusitis (CRS) is a significant health problem. It affects 5–12% of the general population. The causes that underlie the onset of CRS are not yet well known. However, many factors may contribute to its onset, such as environmental factors and the host’s general condition. Medical treatment mainly uses local corticosteroids, nasal irrigation, and antibiotics. In recent years, a new therapeutic approach that employs the use of probiotics emerged. Probiotics have been extensively studied as a therapy for dysbiosis and inflammatory pathologies of various parts of the body. We aimed to examine the studies in vivo and in vitro and clinicals reports in the existing literature to update probiotics’ role in rhinosinusitis chronic medical treatment.
Dynamics of Neuropsychological Symptoms during the Training of Executive Functions in Neurological Patients
Authors: Shcherbakov Nikolai , Varako Nataliya , Kovyazina Maria , Zueva Yulia , Baulina Maria , Skvortsov Anatoliy , Chernikova Daria
Published: December 2021

Executive function disorder rehabilitation in neurological patients is associated with many difficulties. We investigated the effectiveness of group training, proposed by B. Wilson et al., which has the model of frontal lobes functioning by D. T. Stuss as the theoretical background. The study participants were 16 patients with executive function disorder caused by TBI, strokes, and infections. The training was shortened from 9 weeks to 3 and adopted to the conditions of the rehabilitation centre where the study was held. The evaluation of training effectiveness was carried out by the methods of neuropsychological diagnostics proposed by A. R. Luria as well as standardized quantitative tests (CWIT test, Raven test, FAB) and questionnaires (EBIQ) aimed at assessing the state of executive functions and general well-being. In result positive trends, but not reaching the level of significance, were revealed in the performance of all evaluating methods, with the exception of “arithmetic problems” and “inhibitory control” as part of the FAB test. Statistically significant result was obtained concerning such tests as “counting”, “analysis of story pictures”, and index of total uncorrected errors in the CWIT test. Thus, the results of eventual assessment showed positive dynamic of executive functions state.
Genotype Screening and Propagation Techniques of Three Selected Medicinal Plant Species for Production of High-Quality Planting Material
Authors: Fazwa Md Ariff Farah , Kumar Muniandi Sures , Saffie Norhayati , Nabilah Samsul Bahari Syafiqah , Abdullah Zunoliza , Kiong Ling Sui , Mohd Taini Masitah , Aisyah Mohd Hashim Siti Nur , Jamaludin Fadzureena , Abdullah Mohd Zaki
Published: December 2021

Medicinal plants are a vital source of new bioactive compounds due to their ecological biodiversity and varied chemical properties of each species. Phenotypic selection coupled with the evaluation of genotypes based on the chemical profile can be used for the development of a high-yielding variety. However, most of the raw material that has been used for commercial production of herbal products is mainly derived from wild sources, with little knowledge of the quality of genetic materials. Thus, three medicinal species, Chromolaena odorata (Siam weed), Andrographis paniculata (Creat), and Baeckea frutescens (False ru), were chosen based on their significant benefits to human health. These medicinal species have been traditionally used to treat various illnesses, and have been shown to possess anti-inflammatory, antimicrobial, anticancer, and antioxidant properties. This paper highlights the harvesting method and collection of accessions from natural habitats for the selection of superior genotypes. Individual plants having superior phenotypic characteristics and bioactive compounds were identified. We successfully developed appropriate propagation techniques for each species for the mass production of high-yielding planting material. The establishment of breeding activities for these medicinal plants will provide quality raw materials to support the herbal industry in increasing the value of pharmaceutical products. These efforts will also ensure the sustainable production of high-quality planting materials for the establishment of herbal plantations.
Impact of Nitrate and Ammonium Ratios on Flowering and Asexual Reproduction in the Everbearing Strawberry Cultivar Fragaria × ananassa Albion
Authors: Shi Xiaonan , Hernández Ricardo , Hoffmann Mark
Published: December 2021

Ever-bearing (EB) strawberries are long-day cultivars that show perpetual flowering behavior. Compared to June-bearing short-day cultivars, EB cultivars can initiate flowers with less dependency on light and temperature levels. This leads to a more consistent flowering and fruiting pattern, making EB cultivars favorable for areas with long growing seasons. However, this flowering pattern also brings significant challenges to open-field strawberry nurseries. Consistent flower development in EB cultivars frequently leads to increased labor cost for manual flower removal on nursery ground. The alteration of flowering behavior via fertilizer regimes could be a cost-effective tool for strawberry nurseries. However, while it is known that the source of nitrogen (N) impacts strawberry flowering, its effect on strawberry propagation rates needs further investigation. The objective of this study was to investigate the impact of nitrate (NO3−) to ammonium (NH4+) ratio on flower and daughter plant production in the EB strawberry cultivar ‘Albion’ (Fragaria × ananassa c.v. ‘Albion’). Strawberry plants were grown in a completely randomized design under greenhouse conditions (26.6 °C, 16 h photoperiod). Four treatments of NO3−:NH4+ were implemented: (1) 100%:0%; (2) 80%:20%; (3) 60%:40%; (4) 50%:50%. Strawberry plants fertilized with a 60%:40% NO3−:NH4+ ratio produced 17–31% fewer inflorescences than those fertilized with 100%:0% (8.8 ± 1.19) and 80%:20% (10.3 ± 1.85) ratios. The production of daughter plants remained similar in all four treatments. Our results show that increased ratios of ammonium in combination with decreased ratios of nitrate reduce flowering of EB strawberry cultivars, while propagation rates remain consistent. These results could potentially lead to the development of fertilizer regimes for strawberry nurseries to reduce flower production in EB cultivars.
Disparities in COPD Hospitalizations: A Spatial Analysis of Proximity to Toxics Release Inventory Facilities in Illinois
Authors: Brown-Amilian Stacey , Akolade Yussuf
Published: December 2021

Disproportionate distribution of air pollution is a major burden on the health of people living in proximity to toxic facilities. There are over 1000 Toxics Release Inventory (TRI) facilities distributed across the state of Illinois. This study investigates and spatially analyzes the relationship between chronic obstructive pulmonary disease (COPD) hospitalizations and toxic emissions from TRI facilities. In addition, this study investigates the connection between COPD hospitalizations and socioeconomic variables. Accounting for dispersion of air pollution beyond the TRI facilities source was attained using the inverse distance weighting interpolation approach. Multiple statistical methods were used including principal components analysis, linear regression, and bivariate local indicators of spatial association (BiLISA). The results from the linear regression model and BiLISA clustering maps show there is a strong connection between COPD hospitalizations and socioeconomic status along with race. TRI emissions were not statistically significant, but there are three major clusters of high COPD hospitalizations with high TRI emissions. Rural areas also seem to carry a higher burden of pollution-emitting facilities and respiratory hospitalizations.
Urban–Rural Gradients Predict Educational Gaps: Evidence from a Machine Learning Approach Involving Academic Performance and Impervious Surfaces in Ecuador
Authors: Santos-García Fabián , Valdivieso Karina Delgado , Rienow Andreas , Gairín Joaquín
Published: December 2021

Academic performance (AP) is explained by a multitude of factors, principally by those related to socioeconomic, cultural, and educational environments. However, AP is less understood from a spatial perspective. The aim of this study was to investigate a methodology using a machine learning approach to determine which answers from a questionnaire-based survey were relevant for explaining the high AP of secondary school students across urban–rural gradients in Ecuador. We used high school locations to construct individual datasets and stratify them according to the AP scores. Using the Boruta algorithm and backward elimination, we identified the best predictors, classified them using random forest, and mapped the AP classification probabilities. We summarized these results as frequent answers observed for each natural region in Ecuador and used their probability outputs to formulate hypotheses with respect to the urban–rural gradient derived from annual maps of impervious surfaces. Our approach resulted in a cartographic analysis of AP probabilities with overall accuracies around 0.83–0.84% and Kappa values of 0.65–0.67%. High AP was primarily related to answers regarding the academic environment and cognitive skills. These identified answers varied depending on the region, which allowed for different interpretations of the driving factors of AP in Ecuador. A rural-to-urban transition ranging 8–17 years was found to be the timespan correlated with achievement of high AP.
Drought and Subsequent Soil Flooding Affect the Growth and Metabolism of Savoy Cabbage
Authors: Barber Alessa , Müller Caroline
Published: December 2021

An important factor of current climate change is water availability, with both droughts and flooding becoming more frequent. Effects of individual stresses on plant traits are well studied, although less is known about the impacts of sequences of different stresses. We used savoy cabbage to study the consequences of control conditions (well-watered) versus continuous drought versus drought followed by soil flooding and a potential recovery phase on shoot growth and leaf metabolism. Under continuous drought, plants produced less than half of the shoot biomass compared to controls, but had a >20% higher water use efficiency. In the soil flooding treatment, plants exhibited the poorest growth performance, particularly after the “recovery” phase. The carbon-to-nitrogen ratio was at least twice as high, whereas amino acid concentrations were lowest in leaves of controls compared to stressed plants. Some glucosinolates, characteristic metabolites of Brassicales, showed lower concentrations, especially in plants of the flooding treatment. Stress-specific investment into different amino acids, many of them acting as osmolytes, as well as glucosinolates, indicate that these metabolites play distinct roles in the responses of plants to different water availability conditions. To reduce losses in crop production, we need to understand plant responses to dynamic climate change scenarios.
Immunosenescence in Choroidal Neovascularization (CNV)—Transcriptional Profiling of Naïve and CNV-Associated Retinal Myeloid Cells during Aging
Authors: Schlecht Anja , Thien Adrian , Wolf Julian , Prinz Gabriele , Agostini Hansjürgen , Schlunck Günther , Wieghofer Peter , Boneva Stefaniya , Lange Clemens
Published: December 2021

Immunosenescence is considered a possible factor in the development of age-related macular degeneration and choroidal neovascularization (CNV). However, age-related changes of myeloid cells (MCs), such as microglia and macrophages, in the healthy retina or during CNV formation are ill-defined. In this study, Cx3cr1-positive MCs were isolated by fluorescence-activated cell sorting from six-week (young) and two-year-old (old) Cx3cr1GFP/+ mice, both during physiological aging and laser-induced CNV development. High-throughput RNA-sequencing was performed to define the age-dependent transcriptional differences in MCs during physiological aging and CNV development, complemented by immunohistochemical characterization and the quantification of MCs, as well as CNV size measurements. These analyses revealed that myeloid cells change their transcriptional profile during both aging and CNV development. In the steady state, senescent MCs demonstrated an upregulation of factors contributing to cell proliferation and chemotaxis, such as Cxcl13 and Cxcl14, as well as the downregulation of microglial signature genes. During CNV formation, aged myeloid cells revealed a significant upregulation of angiogenic factors such as Arg1 and Lrg1 concomitant with significantly enlarged CNV and an increased accumulation of MCs in aged mice in comparison to young mice. Future studies need to clarify whether this observation is an epiphenomenon or a causal relationship to determine the role of immunosenescence in CNV formation.
Gene Expression Data Mining Reveals the Involvement of GPR55 and Its Endogenous Ligands in Immune Response, Cancer, and Differentiation
Authors: Wnorowski Artur , Wójcik Jakub , Maj Maciej
Published: December 2021

G protein-coupled receptor 55 (GPR55) is a recently deorphanized lipid- and peptide-sensing receptor. Its lipidic endogenous agonists belong to lysoglycerophospholipids, with lysophosphatidylinositol (LPI) being the most studied. Peptide agonists derive from fragmentation of pituitary adenylate cyclase-activating polypeptide (PACAP). Although GPR55 and its ligands were implicated in several physiological and pathological conditions, their biological function remains unclear. Thus, the aim of the study was to conduct a large-scale re-analysis of publicly available gene expression datasets to identify physiological and pathological conditions affecting the expression of GPR55 and the production of its ligands. The study revealed that regulation of GPR55 occurs predominantly in the context of immune activation pointing towards the role of the receptor in response to pathogens and in immune cell lineage determination. Additionally, it was revealed that there is almost no overlap between the experimental conditions affecting the expression of GPR55 and those modulating agonist production. The capacity to synthesize LPI was enhanced in various types of tumors, indicating that cancer cells can hijack the motility-related activity of GPR55 to increase aggressiveness. Conditions favoring accumulation of PACAP-derived peptides were different than those for LPI and were mainly related to differentiation. This indicates a different function of the two agonist classes and possibly the existence of a signaling bias.
Enhanced p53 Levels Are Involved in the Reduced Mineralization Capacity of Osteoblasts Derived from Shwachman–Diamond Syndrome Subjects
Authors: Frattini Annalisa , Bolamperti Simona , Valli Roberto , Cipolli Marco , Pinto Rita Maria , Bergami Elena , Frau Maria Rita , Cesaro Simone , Signo Michela , Bezzerri Valentino , Porta Giovanni , Khan Abdul Waheed , Rubinacci Alessandro , Villa Isabella
Published: December 2021

Shwachman–Diamond syndrome (SDS) is a rare autosomal recessive disorder characterized by bone marrow failure, exocrine pancreatic insufficiency, and skeletal abnormalities, caused by loss-of-function mutations in the SBDS gene, a factor involved in ribosome biogenesis. By analyzing osteoblasts from SDS patients (SDS-OBs), we show that SDS-OBs displayed reduced SBDS gene expression and reduced/undetectable SBDS protein compared to osteoblasts from healthy subjects (H-OBs). SDS-OBs cultured in an osteogenic medium displayed a lower mineralization capacity compared to H-OBs. Whole transcriptome analysis showed significant differences in the gene expression of SDS-OBs vs. H-OBs, particularly in the ossification pathway. SDS-OBs expressed lower levels of the main genes responsible for osteoblastogenesis. Of all downregulated genes, Western blot analyses confirmed lower levels of alkaline phosphatase and collagen type I in SDS-OBs than in H-OBs. Interestingly, SDS-OBs showed higher protein levels of p53, an inhibitor of osteogenesis, compared to H-OBs. Silencing of Tp53 was associated with higher collagen type I and alkaline phosphatase protein levels and an increase in SDS-OB mineralization capacity. In conclusion, our results show that the reduced capacity of SDS-OBs to mineralize is mediated, at least in part, by the high levels of p53 and highlight an important role of SBDS in osteoblast functions.
Fucosylation in Urological Cancers
Authors: Fujita Kazutoshi , Hatano Koji , Hashimoto Mamoru , Tomiyama Eisuke , Miyoshi Eiji , Nonomura Norio , Uemura Hirotsugu
Published: December 2021

Fucosylation is an oligosaccharide modification that plays an important role in immune response and malignancy, and specific fucosyltransferases (FUTs) catalyze the three types of fucosylations: core-type, Lewis type, and H type. FUTs regulate cancer proliferation, invasiveness, and resistance to chemotherapy by modifying the glycosylation of signaling receptors. Oligosaccharides on PD-1/PD-L1 proteins are specifically fucosylated, leading to functional modifications. Expression of FUTs is upregulated in renal cell carcinoma, bladder cancer, and prostate cancer. Aberrant fucosylation in prostate-specific antigen (PSA) could be used as a novel biomarker for prostate cancer. Furthermore, elucidation of the biological function of fucosylation could result in the development of novel therapeutic targets. Further studies are needed in the field of fucosylation glycobiology in urological malignancies.
Galectin-3 Contributes to the Inhibitory Effect of lα,25-(OH)2D3 on Osteoclastogenesis
Authors: Gu Jianhong , Zhang Xueqing , Zhang Chuang , Li Yawen , Bian Jianchun , Liu Xuezhong , Yuan Yan , Zou Hui , Tong Xishuai , Liu Zongping
Published: December 2021

The active form of vitamin D, 1α,25-(OH)2D3, not only promotes intestinal calcium absorption, but also regulates the formation of osteoclasts (OCs) and their capacity for bone mineral dissolution. Gal-3 is a newly discovered bone metabolic regulator involved in the proliferation, differentiation, and apoptosis of various cells. However, the role of galectin-3 (gal-3) in OC formation and the regulatory effects of 1α,25-(OH)2D3 have yet to be explored. To confirm whether gal-3 contributes to the regulatory effects of 1α,25-(OH)2D3 on osteoclastogenesis, osteoclast precursors (OCPs) were induced by macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor κB ligand (RANKL). TRAP staining and bone resorption analyses were used to verify the formation and activation of OCs. qPCR, Western blotting, co-immunoprecipitation, and immunofluorescence assays were used to detect gene and protein expression. The regulatory effects of gal-3 in OC formation after treatment with 1α,25-(OH)2D3 were evaluated using gal-3 siRNA. The results showed that 1α,25-(OH)2D3 significantly increased gal-3 expression and inhibited OC formation and bone resorption. Expression levels of OC-related genes and proteins, matrix metalloproteinase 9 (MMP-9), nuclear factor of activated T cells 1 (NFATc1), and cathepsin K (Ctsk) were also inhibited by 1α,25-(OH)2D3. Gal-3 knockdown attenuated the inhibitory effects of 1α,25-(OH)2D3 on OC formation, activation, and gene and protein expression. In addition, gal-3 was co-localized with the vitamin D receptor (VDR). These data suggest that gal-3 contributes to the osteoclastogenesis inhibitory effect of lα,25-(OH)2D3, which is involved in bone and calcium homeostasis.
Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway
Authors: Luo Jingyi , Sun Pengbo , Zhang Xun , Lin Guanglan , Xin Qilei , Niu Yaoyun , Chen Yang , Xu Naihan , Zhang Yaou , Xie Weidong
Published: December 2021

The microenvironment plays a vital role in tumor progression, and hypoxia is a typical microenvironment feature in nearly all solid tumors. In this study, we focused on elucidating the effect of canagliflozin (CANA), a new class of antidiabetic agents, on hepatocarcinoma (HCC) tumorigenesis under hypoxia, and demonstrated that CANA could significantly inhibit hypoxia-induced metastasis, angiogenesis, and metabolic reprogramming in HCC. At the molecular level, this was accompanied by a reduction in VEGF expression level, as well as a reduction in the epithelial-to-mesenchymal transition (EMT)-related proteins and glycolysis-related proteins. Next, we focused our study particularly on the modulation of HIF-1α by CANA, which revealed that CANA decreased HIF-1α protein level by inhibiting its synthesis without affecting its proteasomal degradation. Furthermore, the AKT/mTOR pathway, which plays an important role in HIF-1α transcription and translation, was also inhibited by CANA. Thus, it can be concluded that CANA decreased metastasis, angiogenesis, and metabolic reprogramming in HCC by inhibiting HIF-1α protein accumulation, probably by targeting the AKT/mTOR pathway. Based on our results, we propose that CANA should be evaluated as a new treatment modality for liver cancer.
Calcium in Neuronal and Glial Response to Axotomy
Authors: Khaitin Andrey
Published: December 2021

Neurotrauma assumes an instant or delayed disconnection of axons (axotomy), which affects not only neurons, but surrounding glia as well. Not only mechanically injured glia near the site of disconnection, especially transection, is subjected to the damage, but also glia that is remote from the lesion site. Glial cells, which surround the neuronal body, in turn, support neuron survival, so there is a mutual protection between neuron and glia. Calcium signaling is a central mediator of all post-axotomy events, both in neuron and glia, playing a critical role in their survival/regeneration or death/degeneration. The involvement of calcium in post-axotomy survival of the remote, mechanically intact glia is poorly studied. The purpose of this review is to sum up the calcium-involving mechanisms in responses of neurons and glial cells to axotomy to show their importance and to give some suggestions for future research of remote glia in this context.
Opioid Receptors and Protonation-Coupled Binding of Opioid Drugs
Authors: Lešnik Samo , Bertalan Éva , Bren Urban , Bondar Ana-Nicoleta
Published: December 2021

Opioid receptors are G-protein-coupled receptors (GPCRs) part of cell signaling paths of direct interest to treat pain. Pain may associate with inflamed tissue characterized by acidic pH. The potentially low pH at tissue targeted by opioid drugs in pain management could impact drug binding to the opioid receptor, because opioid drugs typically have a protonated amino group that contributes to receptor binding, and the functioning of GPCRs may involve protonation change. In this review, we discuss the relationship between structure, function, and dynamics of opioid receptors from the perspective of the usefulness of computational studies to evaluate protonation-coupled opioid-receptor interactions.
Relationship between Serum Kallistatin and Afamin and Anthropometric Factors Associated with Obesity and of Being Overweight in Patients after Myocardial Infarction and without Myocardial Infarction
Authors: Nowicki Grzegorz Józef , Ślusarska Barbara , Polak Maciej , Naylor Katarzyna , Kocki Tomasz
Published: December 2021

Extensive clinical and epidemiological evidence has linked obesity to a broad spectrum of cardiovascular disease (CVD), including coronary disease, heart failure, hypertension, cerebrovascular disease, atrial fibrillation, ventricular arrhythmias, and sudden death. In addition, increasing knowledge of regulatory peptides has allowed an assessment of their role in various non-communicable diseases, including CVD. The study assessed the concentration of kallistatin and afamin in the blood serum of patients after a myocardial infarction and without a cardiovascular event, and determined the relationship between the concentration of kallistatin and afamin and the anthropometric indicators of being overweight and of obesity in these groups. Serum kallistatin and afamin were quantified by ELISA tests in a cross-sectional study of 160 patients who were divided into two groups: study group (SG) (n = 80) and another with no cardiovascular event (CG) (n = 80). Serum kallistatin concentration was significantly higher in the SG (p < 0.001), while the level of afamin was significantly lower in this group (p < 0.001). In addition, a positive correlation was observed in the SG between the afamin concentration and the waist to hip ratio (WHR), lipid accumulation product (LAP) and the triglyceride glucose index (TyG index). In the CG, the concentration of kallistatin positively correlated with the LAP and TyG index, while the concentration of afamin positively correlated with all the examined parameters: body mass index (BMI), waist circumference (WC), hip circumference (HC), waist to hip ratio (WHtR), visceral adiposity index (VAI), LAP and TyG index. Serum kallistatin and afamin concentrations are associated with the anthropometric parameters related to being overweight and to obesity, especially to those describing the visceral distribution of adipose tissue and metabolic disorders related to excessive fatness.
Uterine Adenomyosis Treated by Linzagolix, an Oral Gonadotropin-Releasing Hormone Receptor Antagonist: A Pilot Study with a New ’Hit Hard First and then Maintain’ Regimen of Administration
Authors: Donnez Jacques , Donnez Olivier , Tourniaire Jean , Brethous Michel , Bestel Elke , Garner Elizabeth , Charpentier Sébastien , Humberstone Andrew , Loumaye Ernest
Published: December 2021

(1) Background: The aim of the present pilot study was to study the effect of a new oral gonadotropin-releasing hormone antagonist on adenomyosis. (2) Methods: Eight premenopausal women, aged between 37 and 45 years, presenting with heavy menstrual bleeding, pelvic pain, and dysmenorrhea due to diffuse and disseminated uterine adenomyosis, confirmed by magnetic resonance imaging (MRI), received 200 mg linzagolix once daily for a period of 12 weeks, after which they were switched to 100 mg linzagolix once daily for another 12 weeks. The primary efficacy endpoint was the change in volume of the adenomyotic uterus from baseline to 24 weeks, evaluated by MRI. Secondary efficacy endpoints included the change in uterine volume from baseline to 12 and 36 weeks by MRI, and also weeks 12, 24, and 36 assessed by transvaginal ultrasound (TVUS). Other endpoints were overall pelvic pain, dysmenorrhea, non-menstrual pelvic pain, dyspareunia, amenorrhea, quality of life measures, bone mineral density (BMD), junctional zone thickness, and serum estradiol values. (3) Results: Median serum estradiol was suppressed below 20 pg/mL during the 12 weeks on linzagolix 200 mg, and maintained below 60 pg/mL during the second 12 weeks on linzagolix 100 mg. At baseline, the mean ± SD uterine volume was 333 ± 250 cm3. After 24 weeks of treatment, it was 204 ± 126 cm3, a reduction of 32% (p = 0.0057). After 12 weeks, the mean uterine volume was 159 ± 95 cm3, a reduction of 55% from baseline (p = 0.0001). A similar pattern was observed when uterine volume was assessed by TVUS. Improvements in overall pelvic pain, dysmenorrhea, non-menstrual pelvic pain, dyspareunia, and dyschezia, as well as quality of life measured using the EHP-30 were also observed. Mean percentage BMD loss at 24 weeks was, respectively, −2.4%, −1.3%, and −4.1% for the spine, femoral neck, and total hip. The most common adverse events were hot flushes, which occurred in 6/8 women during the first 12 weeks, and 1/8 women between 12 and 24 weeks. (4) Conclusions: Linzagolix at a dose of 200 mg/day reduced uterine volume, and improved clinically relevant symptoms. Treatment with 100 mg thereafter retains the therapeutic benefits of the starting dose while minimizing side effects. This ‘hit hard first and then maintain’ approach may be the optimal way to treat women with symptomatic adenomyosis.