bluuebunny/crossref_metadata_2025 huggingface dataset:
Association of Late Radiographic Assessment of Lung Edema Score with Clinical Outcome in Patients with Influenza-Associated Acute Respiratory Distress Syndrome
Authors: Shen Hsiao-Chin , Chen Chun-Chia , Chen Wei-Chih , Yu Wen-Kuang , Yang Kuang-Yao , Chen Yuh-Min
Published: November 2023

Background: Influenza virus infection leads to acute pulmonary injury and acute respiratory distress syndrome (ARDS). The Radiographic Assessment of Lung Edema (RALE) score has been proposed as a reliable tool for the evaluation of the opacity of chest X-rays (CXRs). This study aimed to examine the RALE scores and outcomes in patients with influenza-associated ARDS. Methods: Patients who were newly diagnosed with influenza-associated ARDS from December 2015 to March 2016 were enrolled. Two independent reviewers scored the CXRs obtained on the day of ICU admission and on days 2 and 7 after intensive care unit (ICU) admission. Results: During the study, 47 patients had influenza-associated ARDS. Five died within 7 days of ICU admission. Of the remaining 42, non-survivors (N = 12) had higher Sequential Organ Failure Assessment scores (SOFA) at ICU admission and higher day 7 RALE scores than survivors (N = 30). The day 7 RALE score independently related to late in-hospital mortality (aOR = 1.121, 95% CI: 1.014–1.240, p = 0.025). Conclusions: The RALE score for the evaluation of opacity on CXRs is a highly reproducible tool. Moreover, RALE score on day 7 was an independent predictor of late in-hospital mortality in patients with influenza-associated ARDS.
Open versus Transcutaneous (Ultrasound-Guided and Based on Anatomic Landmarks) Tunneled Venous Access to the Right Internal Jugular Vein in Children: A Prospective Single-Center Study
Authors: Kouna Niki , Noutsos George , Koufopoulou Christina , Panagopoulos Dimitrios , Kattamis Antonis
Published: November 2023

Background: The purpose of this study was to compare the immediate and long-term complications that are associated with the utilized techniques for the insertion of indwelling central venous catheters, that is the open surgical technique, the ultrasound-guided technique, and the transcutaneous technique based on external anatomical landmarks in the right internal jugular vein, to a pediatric population. Methods: This was a prospective randomized trial based on a pediatric patient population under 16 years of age of a tertiary pediatric-oncological hospital. The procedure was performed by a medical team with varying experience regarding the percutaneous and open insertion methods. We studied the outcome of our procedure, based on the immediate and delayed complication rate, as well as the needed time in order to complete the procedure and mean duration of line use. Results: The patients that were inserted in our protocol were divided into three subgroups based on the selected technique for the insertion of the central venous catheter. A total number of 88 insertions (25.4%) (out of 346) were based on the technique that was using external anatomical landmarks, 121 insertions were based on the ultrasound-guided transcutaneous technique (34.9%), whereas in 137 cases (39.5%) the open surgical technique was preferred. All cases that were related to catheter re-insertion were excluded from our study. We performed a statistical analysis regarding the catheter dwell time between the three subgroups of patients and no significant difference was recorded. Moreover, the development of thrombosis was investigated, and we noted that a higher percentage of this complication was related to the transcutaneous external landmark and open surgical technique. Also, the incidence of infection was taken into consideration, which manifested an increased incidence when the transcutaneous technique based on external landmarks was used. Conclusions: Ultrasound-guided percutaneous insertion was considered to be a safe and effective technique for the insertion of central venous catheters. Our study also demonstrated a decrease in operating times when performed by operators with increasing expertise, increased preservation of the diameter of the venous lumen, and no increase in complication rates when the ultrasound-guided technique was selected.
Metamaterial-Based Series-Fed Antenna with a High Gain and Wideband Performance for Millimeter-Wave Spectrum Applications
Authors: Esmail Bashar A. F. , Koziel Slawomir , Isleifson Dustin
Published: November 2023

This paper presents a high-gain, wideband series-fed antenna designed for 5G millimeter-wave (MMW) applications. The structure employs a substrate-integrated-waveguide (SIW)-based power splitter and metamaterials (MMs). The power divider functions effectively at 27.5 GHz, exhibiting an impedance bandwidth from 26.9 to 28.6 GHz. The series-fed dipole is assembled on the SIW-based power splitter, incorporating four dipoles with varying lengths and spacing. The dipoles are connected in series on both sides, running in parallel through a microstrip line. Effectively combining the resonances of the series-fed dipoles and the SIW results in a broad impedance bandwidth, ranging from 26.9 GHz to 34.75 GHz. The design has a gain extending from 9 to 10.5 dBi within the operating bandwidth. To improve gain performance without a substantial increase in antenna size, 11 × 6 MM unit cells were positioned in front of the antenna. As a result, the proposed antenna achieves a maximum gain of 14.1 dBi at 30.5 GHz while maintaining an operational bandwidth of 7.85 GHz. Additionally, due to the arrangement of the two MM-based series-fed dipoles, the antenna exhibits symmetrical dual-beam E-plane radiation at ±20° and 28 GHz in the end-fire direction. The developed system was experimentally validated and an excellent agreement between the simulated and measured data was demonstrated.
Augmented Grad-CAM++: Super-Resolution Saliency Maps for Visual Interpretation of Deep Neural Network
Authors: Gao Yongshun , Liu Jie , Li Weihan , Hou Ming , Li Yang , Zhao Huimin
Published: November 2023

In recent years, deep neural networks have shown superior performance in various fields, but interpretability has always been the Achilles’ heel of deep neural networks. The existing visual interpretation methods for deep neural networks still suffer from inaccurate and insufficient target localization and low-resolution saliency maps. To address the above issues, this paper presents a saliency map generation method based on image geometry augmentation and super-resolution called augmented high-order gradient weighting class activation mapping (augmented grad-CAM++). Unlike previous approaches that rely on a single input image to generate saliency maps, this method first introduces the image geometry augmentation technique to create a set of augmented images for the input image and generate activation mappings separately. Secondly, the augmented activation mappings are combined to form the final saliency map. Finally, a super-resolution technique is introduced to add pixel points to reconstruct the saliency map pixels to improve the resolution of the saliency map. The proposed method is applied to analyze standard image data and industrial surface defect images. The results indicate that, in experiments conducted on standard image data, the proposed method achieved a 3.1% improvement in the accuracy of capturing target objects compared to traditional methods. Furthermore, the resolution of saliency maps was three times higher than that of traditional methods. In the application of industrial surface defect detection, the proposed method demonstrated an 11.6% enhancement in the accuracy of capturing target objects, concurrently reducing the false positive rate. The presented approach enables more accurate and comprehensive capture of target objects with higher resolution, thereby enhancing the visual interpretability of deep neural networks. This improvement contributes to the greater interpretability of deep learning models in industrial applications, offering substantial performance gains for the practical deployment of deep learning networks in the industrial domain.
A Clone Selection Algorithm Optimized Support Vector Machine for AETA Geoacoustic Anomaly Detection
Authors: He Qiyi , Wang Han , Li Changyi , Zhou Wen , Ye Zhiwei , Hong Liang , Yu Xinguo , Yu Shengjie , Peng Lu
Published: November 2023

Anomaly in geoacoustic emission is an important earthquake precursor. Current geoacoustic anomaly detection methods are limited by their low signal-to-noise ratio, low intensity, sample imbalance, and low accuracy. Therefore, this paper proposes a clone selection algorithm optimized one-class support vector machine method (CSA-OCSVM) for geoacoustic anomaly detection. First, the interquartile range (IQR), cubic spline interpolation, and time window are designed to amplify the geoacoustic signal intensity and energy change rules to reduce the interference of geoacoustic signal noise and intensity. Secondly, to address the imbalance of positive and negative samples in geoacoustic anomaly detection, a one-class support vector machine is introduced for anomaly detection. Meanwhile, in view of the optimization capabilities of the clone selection algorithm, it is adopted to optimize the hyperparameters of OCSVM to improve its detection accuracy. Finally, the proposed model is applied to geoacoustic data anomaly detection in nine different datasets, which are derived from our self-developed acoustic electromagnetic to AI (AETA) system, to verify its effectiveness. By designing comparative experiments with IQR, genetic algorithm OCSVM (GA-OCSVM), particle swarm optimization OCSVM (PSO-OCSVM), and evaluating the performance of the true positive rate (TPR) and false positive rate (FPR), the experimental results depict that the proposed model is superior to the existing state-of-the-art geoacoustic anomaly detection approaches.
Interaction of Prokineticin Receptors with Accessory Proteins
Authors: Lattanzi Roberta , Miele Rossella
Published: November 2023

G protein-coupled receptors (GPCRs) are transmembrane proteins that mediate the intracellular pathway of signals not only through heterotrimeric GTP-binding proteins (G proteins) but also through their associations with a variety of additional partner proteins. Prokineticin receptors 1 (PKR1) and 2 (PKR2) are new members of the GPCRs whose ligands are the novel chemokines prokineticin 1 (PK1) and prokineticin 2 (PK2). The multiplicity of G proteins coupled to PKRs, the ability of PKR2 to heterodimerize, the interaction of PKR2 with accessory proteins, and the existence of alternative splice isoforms of PKR2/PK2 explain the complexity of the system in the signal transduction pathway and, consequently, in the modulation of various physiological and pathological functions. Knowledge of these mechanisms provides the basis for the development of targeted drugs with therapeutic efficacy in PK-dependent diseases.
Visualization of Movement and Expansion of Coal Reaction Zone by Acoustic Emission Monitoring in Underground Coal Gasification System
Authors: Iriguchi Rika , Ishii Yuma , Hamanaka Akihiro , Su Faqiang , Itakura Ken-ichi , Kodama Jun-ichi , Sasaoka Takashi , Shimada Hideki , Deguchi Gota
Published: November 2023

Underground coal gasification (UCG) is the process of directly recovering energy as combustible gases such as hydrogen and carbon monoxide by combusting unmined coal resources in situ. The UCG process is an invisible phenomenon, in which fracturing activity at high temperature (>1000 °C) in coal seams expands the gasification zone and increases the combustible components of the product gas. However, excessive expansion of the gasification zone may cause environmental problems such as gas leakage, deformation of the surrounding ground, and groundwater pollution. Therefore, visualization of the gasification zone of UCG is required for both improving gasification efficiency and developing UCG systems with low environmental impact. In this study, the large-scale model UCG experiments conducted on a laboratory scale (size: 625 mm × 650 mm × 2792 mm (H × W × L)) were carried out to discuss the visualization of the gasification reaction zone of coal in UCG by Acoustic Emission (AE) technique with uniaxial and triaxial acceleration transducers. As the results of temperature monitoring and AE source location analysis show, AE sources are located near the high-temperature zone (>1000 °C). In addition, the located AE sources move and expand with the movement and expansion of the high-temperature zone. AE measurement can be a useful technique for monitoring the progress of the UCG reaction zone. AE measurement with triaxial sensors is also useful to predict a high-temperature zone though the measurable range, which has to be considered.
Egg Morphology and Chorionic Ultrastructure of Spotted Lanternfly, Lycorma delicatula (White) (Hemiptera: Fulgoridae)
Authors: Powell Jonathan M. , Nixon Laura J. , Lourie Austin P. , Leskey Tracy C. , Walse Spencer S.
Published: November 2023

Knowledge regarding egg morphology can aid the selection of postharvest fumigants for insect control. Accordingly, scanning electron microscopy (SEM) was used to examine eggs of spotted lanternfly (SLF), Lycorma delicatula (White) (Hemiptera: Fulgoridae), a pest recently invasive to the mid-Atlantic region of the United States. As the overwintering life stage of SLF, eggs are deposited on a variety of refugia, including many forestry products that can be distributed geographically via travel, commerce, and/or trade. For fumigation to control SLF, and potentially translate into a viable strategy for limiting the spread of SLF by subject pathways, the fumigant must permeate the chorion to react with biomolecules and/or disrupt cellular processes. SLF chorion was characterized by a porous network of aeropyles localized around the operculum, in cranial and caudal relation to the developing nymph, as well as an interstice between the operculum edge and the opercular rim. The confirmation of chorionic ultrastructure that allows for ready gas exchange warrants further investigation of fumigation efficacy, even for those “non-reactive” fumigants, such as phosphine and hydrogen cyanide, which must overcome the suppression of cellular processes coincident with overwintering.
Spatiotemporal Variation in Vegetation and Its Driving Mechanisms in the Southwest Alpine Canyon Area of China
Authors: Lai Jinlin , Zhao Tianheng , Qi Shi
Published: November 2023

The Southwest Alpine Canyon Area (SACA), a well-known ecological vulnerability region, plays a very important role in China. Identifying the driving force of the spatial heterogeneity of vegetation and the response of interannual vegetation changes to climate change and human activities would be helpful for ecosystem management. Based on the NDVI dataset, the study analyzed the trend of NDVI change from 2000 to 2019 using the Theil–Sen trend analysis and the Mann–Kendal significance test, detected the driving forces of the spatial heterogeneity of NDVI by the means of the geographical detector, and analyzed the relative contribution of climate change and human activities to interannual NDVI changes using residual analysis model. The results showed that, in terms of the spatial distribution, the pattern of NDVI showed that it is higher in the southeast and lower in the northwest region of the SACA. Elevation was the dominant factor influencing the spatial heterogeneity of NDVI, with the explanatory power of 64%, much larger than other factors, and vegetation type, temperature, precipitation, land use type, and soil type were the main factors. In addition, the explanatory power of the dual factor interaction was higher than that of the single factor effect, which showed two kinds of interaction relationships: bivariate enhancement and nonlinear enhancement. In terms of the temporal variation, 85.59% of the study area showed an increasing trend, and only 14.41% of the area showed a decreasing trend. The main factor affecting NDVI changes was human activities, and climate change was the secondary factor, with relative contributions of 71.35% and 28.65%, respectively. The study will promote a better understanding of the complex mechanisms of vegetation changes and provide scientific recommendations for the prevention of vegetation degradation and vegetation restoration in the SACA.
Protecting Hybrid ITS Networks: A Comprehensive Security Approach
Authors: Severino Ricardo , Simão José , Datia Nuno , Serrador António
Published: November 2023

Cooperative intelligent transport systems (C-ITS) continue to be developed to enhance transportation safety and sustainability. However, the communication of vehicle-to-everything (V2X) systems is inherently open, leading to vulnerabilities that attackers can exploit. This represents a threat to all road users, as security failures can lead to privacy violations or even fatalities. Moreover, a high fatality rate is correlated with soft-mobility road users. Therefore, when developing C-ITS systems, it is important to broaden the focus beyond connected vehicles to include soft-mobility users and legacy vehicles. This work presents a new approach developed in the context of emerging hybrid networks, combining intelligent transport systems operating in 5.9 GHz (ITS-G5) and radio-mobile cellular technologies. Two protocols were implemented and evaluated to introduce security guarantees (such as privacy and integrity) in communications within the developed C-ITS hybrid environment. As a result, this work securely integrates G5-connected ITS stations and soft-mobility users through a smartphone application via cellular networks. Commercial equipment was used for this goal, including on-board and roadside units. Computational, transmission and end-to-end latency were used to assess the system’s performance. Implemented protocols introduce an additional 11% end-to-end latency in hybrid communications. Moreover, workflows employing hybrid communications impose, on average, an extra 28.29 ms of end-to-end latency. The proposal shows promise, as it reaches end-to-end times below the latency requirements imposed in most C-ITS use cases.
ICN-Based Enhanced Content Delivery for CDN
Authors: Gao Lei , Zhu Xiaoyong
Published: November 2023

With the rapid growth of internet traffic, the traditional host-to-host TCP/IP architecture is subject to many service limitations faced with content-oriented applications. Various novel network architectures have been proposed to solve these limitations, among which Information-Centric Networking (ICN) is one of the most prominent. ICN features the decoupling of content (service) from the physical devices storing (providing) it through location-independent naming, and offers inherent enhancement to network performance, such as multicast and in-network caching. ICN in-network caching has been extensively studied, and we believe that it may also be the main incentive for ISPs to deploy ICN. A CDN (content delivery network) is a typical content-oriented network paradigm that aims to provide the fast delivery of content. In this paper, we leverage the advantages of the in-network caching of ICN to enhance the content delivery efficiency of CDN by integrating ICN as a service. First, we present our design of a content delivery network enhanced with ICN, called IECDN. Additionally, we formulate a mathematical model to optimize the performance of our proposed design and conduct a series of evaluations. The results indicate that our proposed design provides significant performance gains while reducing bandwidth consumption and shows better resilience to traffic surge.
Polyglycerol-Based Hydrogel as Versatile Support Matrix for 3D Multicellular Tumor Spheroid Formation
Authors: Thongrom Boonya , Tang Peng , Arora Smriti , Haag Rainer
Published: November 2023

Hydrogel-based artificial scaffolds are essential for advancing cell culture models from 2D to 3D, enabling a more realistic representation of physiological conditions. These hydrogels can be customized through crosslinking to mimic the extracellular matrix. While the impact of extracellular matrix scaffolds on cell behavior is widely acknowledged, mechanosensing has become a crucial factor in regulating various cellular functions. cancer cells’ malignant properties depend on mechanical cues from their microenvironment, including factors like stiffness, shear stress, and pressure. Developing hydrogels capable of modulating stiffness holds great promise for better understanding cell behavior under distinct mechanical stress stimuli. In this study, we aim to 3D culture various cancer cell lines, including MCF-7, HT-29, HeLa, A549, BT-474, and SK-BR-3. We utilize a non-degradable hydrogel formed from alpha acrylate-functionalized dendritic polyglycerol (dPG) and thiol-functionalized 4-arm polyethylene glycol (PEG) via the thiol-Michael click reaction. Due to its high multivalent hydroxy groups and bioinert ether backbone, dPG polymer was an excellent alternative as a crosslinking hub and is highly compatible with living microorganisms. The rheological viscoelasticity of the hydrogels is tailored to achieve a mechanical stiffness of approximately 1 kPa, suitable for cell growth. Cancer cells are in situ encapsulated within these 3D network hydrogels and cultured with cell media. The grown tumor spheroids were characterized by fluorescence and confocal microscopies. The average grown size of all tumoroid types was ca. 150 µm after 25 days of incubation. Besides, the stability of a swollen gel remains constant after 2 months at physiological conditions, highlighting the nondegradable potential. The successful formation of multicellular tumor spheroids (MCTSs) for all cancer cell types demonstrates the versatility of our hydrogel platform in 3D cell growth.
Navigating the Complex Landscape of Fibrodysplasia Ossificans Progressiva: From Current Paradigms to Therapeutic Frontiers
Authors: Anwar Saeed , Yokota Toshifumi
Published: November 2023

Fibrodysplasia ossificans progressiva (FOP) is an enigmatic, ultra-rare genetic disorder characterized by progressive heterotopic ossification, wherein soft connective tissues undergo pathological transformation into bone structures. This incapacitating process severely limits patient mobility and poses formidable challenges for therapeutic intervention. Predominantly caused by missense mutations in the ACVR1 gene, this disorder has hitherto defied comprehensive mechanistic understanding and effective treatment paradigms. This write-up offers a comprehensive overview of the contemporary understanding of FOP’s complex pathobiology, underscored by advances in molecular genetics and proteomic studies. We delve into targeted therapy, spanning genetic therapeutics, enzymatic and transcriptional modulation, stem cell therapies, and innovative immunotherapies. We also highlight the intricate complexities surrounding clinical trial design for ultra-rare disorders like FOP, addressing fundamental statistical limitations, ethical conundrums, and methodological advancements essential for the success of interventional studies. We advocate for the adoption of a multi-disciplinary approach that converges bench-to-bedside research, clinical expertise, and ethical considerations to tackle the challenges of ultra-rare diseases like FOP and comparable ultra-rare diseases. In essence, this manuscript serves a dual purpose: as a definitive scientific resource for ongoing and future FOP research and a call to action for innovative solutions to address methodological and ethical challenges that impede progress in the broader field of medical research into ultra-rare conditions.
Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of Cnaphalocrocis medinalis
Authors: Du Hai-Tao , Lu Jia-Qi , Ji Kun , Wang Chu-Chu , Yao Zhi-Chao , Liu Fang , Li Yao
Published: November 2023

The rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), is a notorious pest of rice in Asia. The larvae and adults of C. medinalis utilize specialized chemosensory systems to adapt to different environmental odors and physiological behaviors. However, the differences in chemosensory genes between the olfactory organs of these two different developmental stages remain unclear. Here, we conducted a transcriptome analysis of larvae heads, male antennae, and female antennae in C. medinalis and identified 131 putative chemosensory genes, including 32 OBPs (8 novel OBPs), 23 CSPs (2 novel CSPs), 55 ORs (17 novel ORs), 19 IRs (5 novel IRs) and 2 SNMPs. Comparisons between larvae and adults of C. medinalis by transcriptome and RT-qPCR analysis revealed that the number and expression of chemosensory genes in larval heads were less than that of adult antennae. Only 17 chemosensory genes (7 OBPs and 10 CSPs) were specifically or preferentially expressed in the larval heads, while a total of 101 chemosensory genes (21 OBPs, 9 CSPs, 51 ORs, 18 IRs, and 2 SNMPs) were specifically or preferentially expressed in adult antennae. Our study found differences in chemosensory gene expression between larvae and adults, suggesting their specialized functions at different developmental stages of C. medinalis. These results provide a theoretical basis for screening chemosensory genes as potential molecular targets and developing novel management strategies to control C. medinalis.
Sexual Satisfaction among Lebanese Adults: Scale Validation in Arabic and Correlates Following Two Cross-Sectional Studies
Authors: Abboud Cedric , Fawaz Mirna , Hallit Rabih , Obeid Sahar , Malaeb Diana , Hallit Souheil
Published: November 2023

(1) Background: Sexual satisfaction (SS) is an essential component of quality of life. There is a scarcity of research about sexual satisfaction in Lebanon, a country where discussing sexual issues is still considered a taboo. The present study aimed to assess the reliability and validity of responses to the items in the Arabic version of the Sexual Satisfaction Questionnaire (SSQ), as well as the correlates of sexual satisfaction, among a sample of Lebanese adults. (2) Methods: Two cross-sectional studies were conducted between June and September 2022 with 270 and 359 participants, respectively. (3) Results: The results showed that the Sexual Satisfaction Questionnaire is adequate to be used in Lebanon (McDonald’s ω = 0.90 and 0.86, respectively). Multivariate analysis showed that higher waterpipe dependence (Beta = −0.17) was substantially linked to lower sexual satisfaction, while better emotional intelligence (EI) (Beta = 0.27) and physical activity (Beta = 0.17) were significantly associated with greater sexual satisfaction. (4) Conclusions: The reliability and validity of the responses to the Arabic version of the Sexual Satisfaction Questionnaire were supported by our findings. Also, practical implications for sexual satisfaction enhancement strategies in the Lebanese population might be beneficial since many associated factors are considered to be modifiable.
Walking Speed and Risk of Falling Patients Operated for Selected Malignant Tumors
Authors: Latajka Anna , Stefańska Małgorzata , Woźniewski Marek , Malicka Iwona
Published: November 2023

Background: A literature review reveals that studies on walking and fall occurrences in the context of cancer have predominantly centered on geriatric patients. Nonetheless, cancer patients of all ages are susceptible to such risks. Both cancer and its treatments contribute to significant risk factors for disturbances in walking and falls, encompassing muscle weakness, impaired balance, reduced proprioception, cognitive impairment, and functional limitations. Aim: to assess walking speed and the risk of falls among patients undergoing surgery for the most common malignancies: breast (BU), lung (P), colorectal (DS), and reproductive organs (G). Material and Methods: An observational study was conducted using a cohort design. A total of 176 individuals participated in the study, including 139 cancer patients, who were divided into four groups: BU (N = 30), P (N = 35), DS (N = 35), and G (N = 39), as well as 37 healthy volunteers in the control group (C, N = 37). All participants underwent an assessment of walking speed using BTS G-WALK® and an evaluation of the number of falls and the risk of falling using a Fall Control Card. Results: There was a significant decrease in walking speed after surgery compared to the time before surgery, from 2.7% in the BU group, through 9.3% in the P group, and 19.2% in the DS group, to 30.0% in the G group. At the same time, for groups G and DS, the average walking speed fell below 1.0 m/s, amounting to 0.84 m/s and 0.97 m/s, respectively, in the measurement after the surgery and 0.95 m/s and 1.0 m/s in the follow-up measurement. Falling occurred in all the groups except for the BU group. The created logistic regression model showed that increasing the walking speed measured after the procedure (study 2) by 1 m/s reduces the risk of falling by approximately 500 times (OR = 0.002). Limitations in daily activity were observed in the follow-up examination (study 3) in 75% of patients. Conclusions: Surgical intervention has an impact on walking speed, and being part of the study group influences the risk of falling. Further research is needed to determine the precise risk of falls in cancer patients.
Knowledge, Attitude and Practice of the Community General Practice Teams on Dysphagia Complicated with Aspiration Pneumonia after Stroke
Authors: He Daikun , Shen Xueting , Wang Lina , Pan Zhigang
Published: November 2023

This cross-sectional study investigated the knowledge, attitude and practice (KAP) of community general practice (GP) team members on dysphagia complicated with aspiration pneumonia after stroke in Shanghai between October 2022 and November 2022 using a self-administered questionnaire. A total of 551 questionnaires were collected (mean age: 37.59 ± 8.86 years, 443 (80.40%) females), including 383 (69.51%) physicians. The mean KAP scores were 6.30 ± 1.54 (possible range: 0–12), 40.32 ± 5.11 (possible range: 9–45), and 72.54 ± 13.99 (possible range: 18–90), respectively. Multivariable linear regression analyses suggested that attitude (Coef = 1.29, 95%CI: 1.09–1.50), and holding research funding (Coef = −2.70, 95%CI: −5.00–−0.50) were significantly associated with practice toward dysphagia complicated with aspiration pneumonia after stroke of community GP team members. The structural equation model (SEM) indicated that knowledge had a direct influence on attitude (β = 2.029, p < 0.001) and attitude had a direct impact on practice (β = 0.710, p < 0.001). Additionally, knowledge exerted both direct (β = 0.935, p = 0.016) and indirect effects (β = 1.442, p < 0.001) on practice. In conclusion, this study showed that the community GP team members had poor knowledge, favorable attitudes, and proactive practices. Education and training on the management of dysphagia complicated with aspiration pneumonia after stroke are urgently needed.
Dynamic Changes in Physicochemical and Microbiological Qualities of Coconut Water during Postharvest Storage under Different Conditions
Authors: Detudom Rachatida , Deetae Pawinee , Wei Hu , Boran Hu , Chen Shiguo , Siriamornpun Sirithon , Prakitchaiwattana Cheunjit
Published: November 2023

Coconut is naturally sealed with coconut water inside. Microbial contamination occurs only after the seal is broken during extraction. This study evaluated changes in the microbiological, physicochemical, and chemical properties of coconut water during postharvest storage at ambient and refrigerated temperatures. Initial microbial counts ranged from 2 to 5 log CFU/mL, while physicochemical factors, including total soluble solids (TSSs), pH, and sugar content (4–7 °Brix, 5.0–5.5, 4–6% g/100 mL), were consistent. The dynamic changes in the physicochemical properties of coconut water stored under both conditions exhibited a clear correlation with the increased microbial populations. Fructose was the primary sugar, with citric and malic acids as major acids, while the predominant volatile compounds were ethanol, ethyl acetate, ethyl ester, acetic acid and octanoic acid. Storage conditions led to similar microbial and physicochemical changes, but ambient temperature accelerated spoilage 10 times faster than refrigeration. Sucrose decreased steadily, whereas fructose and glucose remained stable until a precipitous decline coincided with lactic acid bacteria (LAB) reaching >6 log CFU/mL on the final day of storage. Weissella cibaria and Leuconostoc spp. are the main species in coconut water. The presence of specific volatile compounds, including octanoic acid, acetic acid, ethyl acetate, and butyl phenol, is associated with the activities of Lactobacillus, particularly Weissella. There was a clear relationship among microbial groups and populations, total titratable acidity (TTA), and sensory criteria. Remarkably, TTA was closely correlated with total plate count (TPC) (>5 log CFU/mL) and an unacceptable sensory rating.
Non-Destructive Evaluation of White-Flesh Dragon Fruit Decay with a Robot
Authors: Ortiz Coral , Blanes Carlos , Gonzalez-Planells Pablo , Rovira-Más Francisco
Published: November 2023

Dragon fruit is a tropical fruit with significant potential for consumers and producers. The quality assurance of this high-value product is crucial to satisfy consumer expectations. The quality of imported dragon fruit after storage may deteriorate due to inappropriate storage conditions. The firmness of dragon fruit is an essential parameter to estimate its conditions, and it is usually measured by destructive testing. The objective of the present study is to develop and test a non-destructive robotic sensor for assessing dragon fruit quality related to texture deterioration. Sixty white-fresh dragon fruits obtained from a store were divided in two sets of thirty fruits and stored 48 h at different conditions (cold and room storage) to produce deteriorated and consumer-acceptable fruits. First, the fruit samples were assessed non-destructive with the force sensor of a collaborative robot while they were touched. The robot tool is a pad capable of adapting and copying fruit shapes while controlling its hardness with the jamming transition of its internal granular fill. Second, the fruits were evaluated with destructive tests such as fruit firmness, flesh firmness, and soluble solid content. The procedure followed to produce deteriorated and acceptable fruits were confirmed. A discriminant analysis was carried out to segregate the fruit between the two categories according to the non-destructive variables extracted from the sensor. The variables obtained from the robotic first slope (S1) and the difference between the maximum value and the first overshoot (Os) were significant predictors for the separation in the two quality categories. Promising results were obtained with 77.50% of well classified fruit from the model data set, and 84.21% from the validation data set. The use of the robot could be an efficient tool in evaluating the quality of dragon fruit. This process may lead to substantial savings, particularly considering the elevated cost associated with the importation of tropical fruits into the European market.
Enhancing Cowpea Productivity in the Sahel: Exploring Seed Access among Smallholder Farmers in South-Central Niger
Authors: Rabé Mahamane Moctar , Baoua Ibrahim B. , Baributsa Dieudonne
Published: November 2023

Cowpea productivity in West Africa is low due to several challenges, including limited access to high-quality seeds. Despite the development of improved varieties, smallholder farmers face difficulties in accessing certified seeds. This study interviewed 634 households in south-central Niger to assess how farmers access cowpea seeds. Most farmers (94.6%) relied on informal seed systems, with local markets being the primary source (72.8%). The formal seed sector, including the private sector, had a limited role. Farmers typically stored seeds for seven months utilizing pesticides (54.9%) and hermetic methods (42.6%). Interestingly, the price of seeds of improved cowpea varieties in local markets was 1.19 times higher than that of local varieties, but only 25% of the cost of certified seeds. Interventions to strengthen informal seed systems and improve access to credit could enhance the adoption of high-quality cowpea seeds and increase productivity.
UV-B Radiation in the Acclimatization Mechanism of Psidium guajava in Sunlight
Authors: Campos Felipe G. , Dantas Mariana O. , Santos João P. M. , Froes Sophia S. , Gama João P. S. , Boaro Carmen S. F.
Published: November 2023

The ozone layer (O3) is essential to the absorption and blocking of UV-B radiation, preventing a large portion from reaching the Earth’s surface. The degradation of the ozone layer (O3) caused by increased pollution has led to climate change exerting significant influence on natural ecosystems and has resulted in severe stress on the environment, such as an increase in UV-B radiation, which has deleterious effects on plant physiology. UV-B influences the protection pathways that increase compound production, leading to metabolic adjustments and promoting plant acclimatization. This study evaluated whether UV-B application prior to sunlight exposure induces anthocyanin synthesis, photochemical change, and carbohydrate profile modification, contributing to acclimatization in Psidium guajava seedlings. A higher concentration of H2O2 may have stimulated anthocyanin synthesis. Furthermore, greater instantaneous water use efficiency (iWUE), the absence of trehalose—a stress marker, and lower concentrations of glucose, fructose, and sucrose indicate that these plants acclimatize when exposed to full sun (30 days). Seedlings exposed to increased UV-B may be more resistant to the climate. The radiation can aid in water resource management with elevated carbohydrate concentrations. These conditions may enhance the success of P. guajava in the field. Therefore, it is suggested that UV-B application to seedlings of P. guajava promotes effective acclimatization, as it activates anthocyanin synthesis, inhibits trehalose accumulation, and increases iWUE. UV-B radiation, depending on its radiance, can be used as a technique in seedling production that can be implanted in anthropic environments.
Investigation of Fruit Quality and Biochemical Traits of Rosehip (R. canina) Ecotypes in the Aegean Region of Türkiye
Authors: Sagbas Halil Ibrahim
Published: November 2023

Rosehip is a valuable fruit species in particular for rural populations. In this study, fruit quality parameters of 15 wild grown rosehip ecotypes naturally obtained from seeds in the Aegean Region of Türkiye were determined. The fruit weight, fruit firmness, fruit flesh ratio, fruit shape index, fruit skin color (chroma), soluble solid content, vitamin C, total phenolic, total carotenoid, total anthocyanin, total flavonoid and antioxidant capacity were investigated. The results showed great diversity among ecotypes. The fruit weight, fruit firmness, fruit flesh ratio, fruit shape index, fruit skin color (chroma) and soluble solid content were between 2.28 and 3.29 g, 4.70 and 7.12 N, 69.34 and 81.67%, 0.97 and 1.07, 53.04 and 60.71 and 18.87 and 21.28%, respectively. The total antioxidant capacity was found to be 15.78–28.17 mg AAE/g in a DPPH assay. The vitamin C content of rosehip fruits was measured as 507–621 mg/100 g. Among ecotypes, A-15 gave the biggest fruits, A-1 had the highest soluble solid content and A-13 had the highest vitamin C content. These results suggested that some ecotypes showed more potent bioactive properties than other ecotypes, mainly related to the variations in the antioxidant capacity and bioactive content between ecotypes. Overall, this study provides additional insight into investigating the genotype exhibition of multifunctional bioactive properties.
Effects of Tactile Stimulation Using an Assortment of Natural Elements on the Psychophysiological Responses of Adults
Authors: Kim Yun-Jin , Choi Soo-Wan , Park Sin-Ae
Published: November 2023

Contact with the natural environment has positive effects on physical and mental health and well-being. This study aimed to investigate the effects of tactile stimulation on the psychophysiological responses of adults, using natural gardening elements. The participants were 30 adults (20–60 years old). The participants received tactile stimulation by touching five natural elements with their hands and feet, and tactile stimulation for each natural element was performed for 90 s. Geranium, tiny ardisia, decomposed granite soil, log hardwood, and culture soil were used as tactile stimulation factors for the hand, and moss, grass, pebble, bark, and culture soil were used as tactile stimulation factors for the foot. Oxyhemoglobin (oxy-Hb) concentrations in the prefrontal cortex during the stimulation as well as blood pressure and pulse rate after each activity were measured. Additionally, the semantic differential method was used to evaluate the psychological effects of contact with the elements on the participants. Compared to before tactile stimulation, the oxy-Hb concentration related to prefrontal lobe cortical activity significantly decreased in some sections using tiny ardisia, log hardwood, and culture soil on the hands, and using grass, moss, pebble, and bark on the feet. Blood pressure also showed a significant decrease after tactile stimulation using geranium, tiny ardisia, and log hardwood. The findings of this study suggest that tactile stimulation using natural gardening elements could be a significant intervention in inducing physiological stability and reducing stress by calming the activity of the prefrontal cortex.
Grape Yield Prediction Models: Approaching Different Machine Learning Algorithms
Authors: Andrade Caio Bustani , Moura-Bueno Jean Michel , Comin Jucinei José , Brunetto Gustavo
Published: November 2023

Efficient marketing of winegrapes involves negotiating with potential buyers long before the harvest, when little is known about the expected vintage. Grapevine physiology is affected by weather conditions as well as by soil properties and such information can be applied to build yield prediction models. In this study, Partial Least Squares Regression (PLSR), Cubist (CUB) and Random Forest (RF) algorithms were used to predict yield from imputed weather station data and soil sample analysis reports. Models using only soil variables had the worst general results (R2 = 0.15, RMSE = 4.16 Mg ha−1, MAE = 3.20 Mg ha−1), while the use of only weather variables yielded the best performance (R2 = 0.52, RMSE = 2.99 Mg ha−1, MAE = 2.43 Mg ha−1). Models built with CUB and RF algorithms showed signs of overfitting, yet RF models achieved the best average results (R2 = 0.58, RMSE = 2.85 Mg ha−1, MAE = 2.24 Mg ha−1) using only weather variables as predictors. Weather data imputation affected RF and CUB models more intensely while PLSR remained fairly insensitive. Plant age, yield level group, vineyard plot, May temperatures, soil pH and exchangeable concentrations of Zn, Cu, K and Mn were identified as important predictors. This exploratory work offers insights for future research on grape yield predictive modeling and grouping strategies to obtain more assertive results, thus contributing to a more efficient grapevine production chain in southern Brazil and worldwide.
Building Consensus on the Point-of-Care Ultrasound Skills Required for Effective Healthcare Service Delivery at District Hospitals in South Africa: A Delphi Study
Authors: Mans Pierre-Andre , Yogeswaran Parimalaranie , Adeniyi Oladele Vincent
Published: November 2023

Background: Despite the widespread availability of ultrasound machines in South African district hospitals, there are no guidelines on the competency in point-of-care ultrasound (POCUS) use required by generalist doctors in this setting. This study aimed to define the required POCUS competencies by means of consensus via the Delphi method. Methods: An online Delphi process was initiated in June 2022, using the existing American Academy of Family Physicians’ ultrasound curriculum (84 skillsets) as the starting questionnaire. Panelists were selected across the country, including two from district hospitals in each province and two from each academic family medicine department in South Africa (N = 36). In each iterative round, the participants were asked to identify which POCUS skillsets were essential, optional (region-specific), or non-essential for South African district hospitals. This process continued until consensus (>70% agreement) was achieved on all of the skillsets. Results: Consensus was achieved on 81 of the 84 skillsets after 5 iterative rounds (96.4%), with 3 skillsets that could not achieve consensus (defined as <5% change over more than 2 consecutive rounds). The final consensus identified 38 essential, 28 optional, and 15 non-essential POCUS skillsets for the South African district hospital context. Conclusions: The list of essential POCUS skillsets provided by this study highlights the predominance of obstetric- and trauma-based skillsets required for generalist healthcare workers in South African district hospitals. The findings will require priority setting and revalidation prior to their implementation across the country.
The Impact of a Cypovirus on Parental and Filial Generations of Lymantria dispar L.
Authors: Akhanaev Yuriy B. , Pavlushin Sergey V. , Kharlamova Daria D. , Odnoprienko Daria , Subbotina Anna O. , Belousova Irina A. , Ignatieva Anastasia N. , Kononchuk Anastasia G. , Tokarev Yuri S. , Martemyanov Vyacheslav V.
Published: November 2023

Recently, we found that the spongy moth Lymantria dispar L. is susceptible to infection by a Dendrolimus sibiricus cytoplasmic polyhedrosis virus (DsCPV-1). In the present study, we evaluated the pathogenicity of DsCPV-1 against L. dispar larvae and its impact on surviving insects after the infection. Offspring of virally challenged insects were tested for susceptibility to a stress factor (starvation). In addition, we used light microscopy and quantitative polymerase chain reaction (qPCR) to test the ability of DsCPV-1 to be transmitted vertically. We found insect mortality of the L. dispar parents following the infection was positively associated with DsCPV-1 dose. DsCPV-1 was lethal to second-instar L. dispar larvae with a 50% lethal dose (LD50) of 1687 occlusion bodies per larva. No vertical transmission of DsCPV-1 to offspring larvae was detected, while the majority of insect deaths among offspring larvae were caused by microsporidia (Vairimorpha lymantriae), which was harbored by the parents. The offspring of virally challenged parents exhibited a higher number of detected microsporidia compared to the control. Our findings suggest that the application of DsCPV-1 is effective in controlling pests in terms of transgenerational impact following virus exposure.