Effect of Adding Different Concentrations of Mix-Oil Solution to Drinking Water of Broiler Chickens Ross 308 and Breeders at Elevated Temperatures on Blood Biochemical Characteristics and Oxidative Enzymes at the Age of 14 Days
Authors:   Department of Animal production, College of Agriculture, University of Kerbala, Iraq., Nafel Nihad Mohammed , Rabee Doaa Adil ,   Department of Animal production, College of Agriculture, University of Kerbala, Iraq., Oleiwi Ghufran Hasan ,   Department of Animal production, College of Agriculture, University of Kerbala, Iraq., Al-Khafaji Pro. Dr. Fadhil Rasool Abbas ,   Department of Animal production, Al-Qasim Green University, al-musodi Mohanad fadhl Hussain ,   Department of Animal production, College of Agriculture, University of Kerbala, Iraq., Hamed Mustafa Hadi ,   Department of Animal production, College of Agriculture, University of Kerbala, Iraq.
Published: December 2024

This study was conducted at Al-Anwar Poultry Station located in Babil Governorate for a period of 35 days, from 10/7/2022 to 14/8/2022, to demonstrate the effect of the mix-oil solution added to the drinking water of broiler chickens on some blood biochemical characteristics and oxidation enzymes at the age of 14. day and under conditions of heat stress, where 300 Ross308 broiler chicks were used, one day old, unsexed, and the average weight of the chicks was 40 gm. The mix-oil was added to the drinking water from the first day, as follows: T1: control treatment without addition, T2: adding 0.25 ml of mix-oil / liter of water, T3: adding 0.50 ml of mix-oil / liter of water, T4: adding 0.75 ml of mix-oil / liter of water, T5: Add 1 ml of mix-oil / liter of water. The experimental birds were exposed to periodic temperatures (28-35-28).

The most important results we obtained are summarized as follows:

A highly significant (p≤0.01) increase in the blood serum glucose concentration of the T5-treated birds compared to the control treatment. A significant increase was also observed (p≤0.01) in the concentration of total protein, albumin and globulin in the T4 treatment compared to the control treatment, while serum cholesterol was observed. The results of our experiment witnessed a "significant" rise and within the normal limits in the concentration of cholesterol for T4-treated birds on T5, T3, and T2 treatments, but it was "significantly similar" in its concentration with the T1 control treatment.
The results showed that there was a highly significant increase (p≤0.01) in the rate of triglycerides for the T4 treatment compared to "with all treatments, while high-density lipoproteins (HDL) and low-density lipoproteins (LDL) witnessed a significant decrease (p≤0.01) in all addition treatments compared to" control treatment.
There was a "significant" decrease (P≤ 0.01) for all addition treatments in the concentration of ALT enzyme compared to the "control treatment", while the T3 treatment had a significant (P≤ 0.01) superiority in the concentration of AST and GSH-PX enzyme compared with the control treatment, while the concentration of the enzyme CAT value decreased in all addition treatments compared to the control treatment, and the rate of MDA increased in the blood serum of T2 treatment birds compared to the control, which recorded the lowest measured rates, but it did not differ significantly with the T5 treatment in its concentration.