bluuebunny/crossref_metadata_2025 huggingface dataset:
Family Functioning and Multiple Sclerosis: Study Protocol of a Multicentric Italian Project
Authors: Di Tella Marialaura , Perutelli Virginia , Miele Giuseppina , Lavorgna Luigi , Bonavita Simona , De Mercanti Stefania Federica , Streito Lidia Mislin , Clerico Marinella , Castelli Lorys
Published: June 2021

Multiple Sclerosis (MS) is a chronic inflammatory and neurodegenerative disease, which not only affects physical functioning, but is also associated with cognitive impairments and great psychological distress. The combination of those symptoms may have negative consequences on the family functioning of patients with MS, with detrimental effects on both marital relationships and parental bonding. Furthermore, the presence of individual characteristics and of an adequate social support may also contribute to the quality and endurance of family relationships. Particularly, high levels of alexithymia, a personality trait that affects the recognition of a person's own emotions, have been associated with reduced interpersonal communication skills and enhanced anxiety/depressive symptoms. Therefore, the main aim of the present study is to provide an in-depth evaluation of family functioning and related factors in patients with MS and their families. In order to reach this goal, the perceived quality of family functioning, dyadic relationships, and parental bonding will be first investigated. Secondly, the possible associations between the quality of family relationships and the presence of alexithymia, psychological distress, and perceived social support will be examined. Patients with MS and their families who will consent to take part in the study will be asked to provide sociodemographic and clinical information, and to complete a series of questionnaires, presented and uploaded on an online dedicated platform. The final sample will be made up of 300 families, consecutively recruited from the Italian medical centers involved in the project. The results of the present study will shed light on the family functioning of patients with MS, through a comprehensive assessment of the main factors that are associated with family dynamics. A holistic evaluation of those aspects can help clinicians and researchers understand family dynamics in MS population better.
Internet Use and Problematic Use in Seniors: A Comparative Study in Switzerland and Poland
Authors: Rochat Lucien , Wilkosc-Debczynska Monika , Zajac-Lamparska Ludmila , Rothen Stéphane , Andryszak Paulina , Gaspoz Julie , Colombo Laura , Khazaal Yasser , Achab Sophia
Published: June 2021

Background: Seniors have been only little considered in studies examining problematic internet use and associated health issues, although they may present risk factors that make them particularly vulnerable for the development of problematic internet use.

Objectives: (1) To compare Internet use and problematic use among seniors in Switzerland and Poland; (2) To examine the relationships between problematic internet use, impulsivity traits and well-being as previous studies showed that internet can be used to cope with negative emotions or life dissatisfaction.

Methods: A cross-sectional survey conducted between June 2016 and April 2017 with 264 older internet users aged above 60 years old recruited in Switzerland (88) and Poland (176) assessing sociodemographic variables, online activities, problematic internet use, impulsivity traits and well-being.

Results: The two groups differed in their reported online activities in that Polish participants reported more searching for information and buying, whereas Swiss participants reported significantly greater problematic internet use than Polish participants. Finally, a multiple linear regression analysis performed on the whole sample indicated that lower well-being and being a Swiss participant were both significantly associated with greater problematic internet use, after age, gender, level of education, impulsivity traits have been controlled for.

Discussion: Swiss seniors showed a more problematic internet use than Polish participants who focused more in their online activities on utility use of internet. The relationships between problematic internet use and well-being suggest that older adults use internet mainly to cope with negative emotion or life dissatisfaction. Socio-cultural differences that could account for these group differences as well as difference with young adults are discussed.
Emotional Reactivity and Family-Related Factors Associated With Self-Injurious Behavior in Adolescents Presenting to a Child and Adolescent Psychiatric Emergency Service
Authors: Kandsperger Stephanie , Jarvers Irina , Ecker Angelika , Schleicher Daniel , Madurkay Joseph , Otto Alexandra , Brunner Romuald
Published: June 2021

Background: Adolescents presenting in a child and adolescent psychiatric emergency service show various psychiatric disturbances, most commonly suicidal ideation, suicide attempts, and non-suicidal self-injury (NSSI). It was postulated that especially disturbed emotion regulation contributes to self-injurious behavior of young people. This study aims to investigate the relevance of emotional reactivity (ER), as part of emotion regulation, during an acute crisis, how it relates to self-injurious behavior reinforcement and how a family as well as peers' history of self-injurious behavior are associated with self-injurious behavior of presenting adolescents. Additionally, crisis-triggering background factors were evaluated from the perspective of patients and their caregivers.

Methods: A consecutive sample of 86 adolescents aged 11–18 years presenting to the emergency outpatient department due to self-injurious thoughts and behavior received a pretreatment psychiatric evaluation. Among other psychometric measures and structured clinical interviews, ER was measured via the Emotion Reactivity Scale (ERS). Family-related aspects were collected both through evaluation of history and through questionnaires filled in by custodians or parents.

Results: Data analysis revealed that suicidal ideation was significantly related to family history with self-injurious behavior in comparison with a family background without such a history. A significant positive correlation was apparent between the ERS sensitivity score and occurrence of NSSI within the past year. A relationship between the ERS and distinct types of reinforcement as a motivation factor for NSSI was found. Post-hoc tests revealed a significant difference between boys and girls when no positive peers' history is present with boys having lower ERS scores than girls, but no difference when both groups had friends engaging in self-injurious behavior. There was only moderate agreement between parents and their children in naming reasons for the current crisis involving NSSI.

Conclusion: Emotional regulation, especially ER, has an influence on patients' acute psychiatric symptomatology and when experiencing an acute crisis should be brought into focus early at psychiatric assessment. A history of self-injurious behavior taken from patient's family members and close circle of friends and agreement on reasons for the crisis should be routinely included in the exploration of a patient presenting with self-injurious behavior.
Public Continuum Beliefs for Different Levels of Depression Severity
Authors: Makowski Anna C. , Schomerus Georg , von dem Knesebeck Olaf
Published: June 2021

Introduction: The notion that depression is a disorder that moves along a continuum is well-established. Similarly, the belief in the continuity of mental illness is considered an important element in the stigma process. Against this background, it is the aim of this study to examine whether public continuum beliefs vary with the severity of depressive symptoms.

Methods: Analyses were based on computer-assisted telephone interviews (CATIs) conducted in winter 2019/2020 in Germany (N = 1,009, response rate 46.8%). Using three vignettes representing mild, moderate, and severe depressive symptoms, beliefs regarding the continuity of symptoms, specifically a fundamental difference, were assessed with seven items. Sociodemographic characteristics and own experiences with depression (affliction and contact) were introduced as covariates.

Results: Significant differences between the three groups of severity were found for the majority of the items measuring continuum beliefs or perceived fundamental difference. However, only few items showed a linear trend indicating a parallel between symptom severity and beliefs. Multivariate regression models showed that a moderate degree of depression was positively associated with stronger continuum beliefs but also with greater perceived difference compared to the mild degree, while no significant associations emerged for the severe vignette.

Limitations: Although a comparison of our sample with official statistics supports the external validity, we cannot rule out a selection bias. It is arguable in how far short case vignettes convey a holistic picture of a person affected by depressive symptoms.

Conclusion: Our results do not indicate a parallel between symptom severity and public continuum beliefs.
Does Fecal Microbiota Transplant Have a Role in Treating Recurrent Clostridioides difficile Infection in Rural Hospitals?
Authors: Vedala Krishna , Sobash Philip , Shah Parth , Kamoga Gilbert-Roy
Published: June 2021

Clostridioides difficile infection possesses a significant economical burden, specifically in the inpatient and rural settings. Fecal Microbiota Transplant has been used for treatment of recurrent Clostridioides difficile but its utility is limited by current guidelines and resources. We conducted a retrospective chart review to evaluate the financial benefit of using Fecal Microbiota Transplant after first recurrence of Clostridioides difficile infection. We found that while its use was restricted, on average Fecal Microbiota Transplant can save $11,603.49 per patient. In conclusion, our study shows that using Fecal Microbiota Transplant could prove to be economically beneficial in treating recurrent CDI in rural hospitals.
Urban-Rural Disparities in Dental Services Utilization Among Adults in China's Megacities
Authors: Qi Xiang , Qu Xiaomin , Wu Bei
Published: June 2021

Objective: China's dental care system is bifurcated between urban and rural areas. However, very few studies have examined the dental services utilization inequities in China's megacities, particularly in these urban and rural areas. This study aims to examine the urban-rural disparities in dental services utilization among adults living in China's megacities based on the Andersen dental services utilization model.

Methods: This study used data from 4,049 residents aged 18–65 who participated in the “2019 New Era and Living Conditions in Megacities Survey.” Multivariate logistic regressions were employed to examine the associations between place of residence and dental services utilization for individuals from ten megacities in China. Predisposing variables (age, gender, marital status, living arrangement, and education), enabling variables (socioeconomic status, occupational status, income, insurance coverage, health attitude, and health behavior), and need variables (self-rated health, oral health status, gum bleeding) were controlled for.

Results: The mean age of the 4,049 adults was 45.2 (standard deviation = 13.0), and 30.4% (n = 1,232) had no dental visits at all. Adults who resided in urban areas were more likely to use dental services [odds ratio (OR) = 1.57, 95% confidence interval (CI) = 1.30 to 1.91] than those residing in rural areas after controlling for key covariates. Factors associated with higher odds of visiting dentists include having a higher income (OR = 1.44, P < 0.001), higher education level (OR = 1.53, P = 0.042), being covered by insurance for urban residents/employees (OR = 1.49, P = 0.031), having a positive attitude toward healthy diets (OR = 1.43, P < 0.001), attending regular physical examination (OR = 1.66, P < 0.001), having more tooth loss (OR = 1.05, P < 0.001), and having frequent gum bleeding (OR = 2.29, P < 0.001).

Conclusion: The findings confirm that place of residence is associated with dental services utilization while adjusting for key covariates. Despite rapid economic development in China, many adults had never visited dentists at all. More efforts should be taken to encourage widespread dental care, such as providing more dental coverage and better access to dental care services.
Characterizing Spectral Channels of Visible Emission Line Coronagraph of Aditya-L1
Authors: Patel Ritesh , Megha A. , Shrivastav Arpit Kumar , Pant Vaibhav , Vishnu M. , Sankarasubramanian K. , Banerjee Dipankar
Published: June 2021

Aditya-L1 is India’s first solar mission with the Visible Emission Line Coronagraph (VELC), which consists of three spectral channels taking high-resolution spectroscopic observations of the inner corona up to 1.5 Rʘ at 5,303, 7,892, and 10,747 Å. In this work, we present a strategy for the slit width optimization of the VELC using synthetic line profiles by taking into account the instrument characteristics and coronal conditions for log(T) varying from 6 to 6.5. The synthetic profiles are convolved with simulated instrumental scattered light and noise to estimate the signal-to-noise ratio (SNR), which will be crucial to designing the future observation plans. We find that the optimum slit width for VELC turns out to be 50 μm, providing sufficient SNR for observations in different solar conditions. We also analyzed the effect of plasma temperature on the SNR at different heights in the VELC field of view for the optimized slit width. We also studied the expected effect of the presence of a CME on the spectral channel observations. This analysis will help to plan the scientific observations of VELC in different solar conditions.
Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle
Authors: Longeri Maria , Russo Valeria , Strillacci Maria Giuseppina , Perillo Antonella , Carisetti Michela , Cozzi Maria Cristina , Neola Benedetto , Roperto Sante
Published: June 2021

Blood samples from 260 unrelated cattle (132 animals affected by papillomavirus-associated bladder tumors and 128 healthy) were genotyped using the classic polymerase chain reaction/restriction fragment length polymorphism method to screen MHC class II bovine leukocyte antigen-DRB3. 2 polymorphism. The DRB3*22 allele was significantly (p≤ 0.01) detected in healthy cattle, thus appearing to have a negative association (protective effect) with virus infection of the urinary bladder known to represent a bladder tumor risk for cattle living free at pasture. Considering the two sequence alleles identified in animals carrying DRB3*22, DRB3*011:01 allele from samples of animals harboring the unexpressed bovine papillomaviruses (BPV)-2E5gene was characterized by amino acid residues believed to have a protective effect against BPV infection such as arginine at position 71 (R71) in pocket 4, histidine at position 11 (H11) in pocket 6, and both glutamine at position 9 (Q9) and serine at position 57 (S57) in pocket 9 of the antigen-binding groove. The DRB3*011:02v allele from affected animals was characterized by amino acids believed to be susceptibility residues such as lysine (K71), tyrosine (Y11), glutamic acid (E9), and aspartic acid (D57) in these pockets. These results suggest that animals harboring the DRB3*011:01 allele may have a lower risk of BPV infection and, consequently, a reduced risk of bladder tumors.
Detection of Anaplasma Phagocytophilum in Horses With Suspected Tick-Borne Disease in Northeastern United States by Metagenomic Sequencing
Authors: Subbiah Murugan , Thirumalapura Nagaraja , Thompson David , Kuchipudi Suresh V. , Jayarao Bhushan , Tewari Deepanker
Published: June 2021

Metagenomic sequencing of clinical diagnostic specimens has a potential for unbiased detection of infectious agents, diagnosis of polymicrobial infections and discovery of emerging pathogens. Herein, next generation sequencing (NGS)-based metagenomic approach was used to investigate the cause of illness in a subset of horses recruited for a tick-borne disease surveillance study during 2017–2019. Blood samples collected from 10 horses with suspected tick-borne infection and five apparently healthy horses were subjected to metagenomic analysis. Total genomic DNA extracted from the blood samples were enriched for microbial DNA and subjected to shotgun next generation sequencing using Nextera DNA Flex library preparation kit and V2 chemistry sequencing kit on the Illumina MiSeq sequencing platform. Overall, 0.4–0.6 million reads per sample were analyzed using Kraken metagenomic sequence classification program. The taxonomic classification of the reads indicated that bacterial genomes were overrepresented (0.5 to 1%) among the total microbial reads. Most of the bacterial reads (~91%) belonged to phyla Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Cyanobacteria and Tenericutes in both groups. Importantly, 10–42.5% of Alphaproteobacterial reads in 5 of 10 animals with suspected tick-borne infection were identified as Anaplasma phagocytophilum. Of the 5 animals positive for A. phagocytophilum sequence reads, four animals tested A. phagocytophilum positive by PCR. Two animals with suspected tick-borne infection and A. phagocytophilum positive by PCR were found negative for any tick-borne microbial reads by metagenomic analysis. The present study demonstrates the usefulness of the NGS-based metagenomic analysis approach for the detection of blood-borne microbes.
Characterization and Use of a Crop-Residue-Based Mat Mulch in the Production of Pepper (Capsicum annuum) during Dry Season
Authors: Hernández-Aranda Víctor , Rojas-Tortolero Diego , Álvarez-Barreto José , Arias-Vega Carlos , Proaño-Saraguro Jaime , Portalanza-Chavarria Alexandra , Sosa Daynet
Published: June 2021

Agricultural mulches from plant waste constitute an ecological alternative due to their capacity to biodegrade and incorporate into the soil. This work aimed at evaluating, during a drought, the effects of a mat mulch, based on banana rachis and rice straw, on bell pepper (Capsicum annuum) production, and to characterize the material, both mechanically (traction resistance) and physical-chemically (water retention capacity, organic and inorganic components). Two contrasting irrigation regimes were used to determine the effect of the mat mulch on edaphic and productive parameters such as plant height, basal stem diameter, number of fruits and flowers, harvest, plant biomass, macro- and micronutrients in leaves, weeds/m2, and soil temperature. Treatments with the mat mulch resulted in increased height, number of fruits and flowers, biomass, and P, Zn and Cu concentrations. The mat mulch contributes to soil water retention, improves pepper agronomic variables response in plant’s height, basal stem diameter, and the number of flowers and fruits, especially with a low water irrigation regime. Furthermore, a 95% reduction in weed/m2, and soil temperature (18 °C) compared to air temperature (26.8 °C) was found. The characteristics of the waste-based mat mulch in soils offer new possibilities for environmentally friendly, efficient, and sustainable agricultural practices.
The Potential of Biochar Made from Agricultural Residues to Increase Soil Fertility and Microbial Activity: Impacts on Soils with Varying Sand Content
Authors: Brtnicky Martin , Hammerschmiedt Tereza , Elbl Jakub , Kintl Antonin , Skulcova Lucia , Radziemska Maja , Latal Oldrich , Baltazar Tivadar , Kobzova Eliska , Holatko Jiri
Published: June 2021

Different types of soil respond variably to biochar amendment. Soil structure and fertility are properties which strongly affect the impacts of biochar on soil fertility and microbial activity. A pot experiment with lettuce was conducted to verify whether biochar amendment is more beneficial in sandy soil than in clay soil. The nutrient content (carbon and nitrogen), microbial biomass carbon, soil respiration, metabolic quotient, and plant biomass yield were determined. The treatments were prepared by mixing silty clay loam (Haplic Luvisol) with a quartz sand in ratios of 0%, 20%, 40%, 60%, 80%, and 100% of sand; the same six treatments were prepared and amended with biochar (12 treatments in total). Soil carbon and nitrogen, microbial biomass carbon, and soil respiration were indirectly dependent on the descending sand ratio, whereas the metabolic quotient increased with the ascending sand ratio. The biochar’s effects were positive for total carbon, microbial biomass carbon, metabolic quotient, and plant biomass in the sand-rich treatments. The maximum biochar-derived benefit in crop yield was found in the 100% sand + biochar treatment, which exhibited 24-fold (AGB) and 11-fold (root biomass) increases compared to the unamended treatment. The biochar application on coarse soil types with lower fertility was proven to be favorable.
Urban Organic Waste for Urban Farming: Growing Lettuce Using Vermicompost and Thermophilic Compost
Authors: Schröder Corinna , Häfner Franziska , Larsen Oliver Christopher , Krause Ariane
Published: June 2021

A transformation towards sustainable food production requires improved circular nutrient management. Urban organic waste contains relevant nutrients and organic matter, yet only 4% of global urban nitrogen (N) and phosphorus (P) sources are presently recycled. One recycling approach is the composting of urban wastes for urban horticulture. We characterized compost from various urban waste fractions and assessed their fertilizer value in a pot trial with lettuce plants. Seven treatments were investigated: food waste vermicompost with coir and paperboard bedding material, thermophilic compost from green waste and human feces, two references with mineral fertilization and a sand control. The lettuce yield and total uptake of P, potassium (K), calcium (Ca), and magnesium (Mg) were highest in plants grown in coir-based vermicompost. The fecal compost led to the highest shoot P and K content, but the shoot uptake of Ca and Mg were lower than in the other treatments. All composts required additional N for lettuce growth. In conclusion, urban waste-derived vermicompost and fecal compost demonstrate a high delivery rate of plant-available Ca, Mg, P, and K. Research is needed on macronutrient availability and alternative N sources for the substitution of synthetic fertilization. These findings support the production of urban waste composts, furthering efforts in nutrient recycling.
Comparative Metagenomic Analysis of Chicken Gut Microbial Community, Function, and Resistome to Evaluate Noninvasive and Cecal Sampling Resources
Authors: Kang Kelang , Hu Yan , Wu Shu , Shi Shourong
Published: June 2021

When conducting metagenomic analysis on gut microbiomes, there is no general consensus concerning the mode of sampling: non-contact (feces), noninvasive (rectal swabs), or cecal. This study aimed to determine the feasibility and comparative merits and disadvantages of using fecal samples or rectal swabs as a proxy for the cecal microbiome. Using broiler as a model, gut microbiomes were obtained from cecal, cloacal, and fecal samples and were characterized according to an analysis of the microbial community, function, and resistome. Cecal samples had higher microbial diversity than feces, while the cecum and cloaca exhibited higher levels of microbial community structure similarity compared with fecal samples. Cecal microbiota possessed higher levels of DNA replicative viability than feces, while fecal microbiota were correlated with increased metabolic activity. When feces were excreted, the abundance of antibiotic resistance genes like tet and ErmG decreased, but some antibiotic genes became more prevalent, such as fexA, tetL, and vatE. Interestingly, Lactobacillus was a dominant bacterial genus in feces that led to differences in microbial community structure, metabolism, and resistome. In conclusion, fecal microbiota have limited potential as a proxy in chicken gut microbial community studies. Thus, feces should be used with caution for characterizing gut microbiomes by metagenomic analysis.
Characteristics of High-Level Aminoglycoside-Resistant Enterococcus faecalis Isolated from Bulk Tank Milk in Korea
Authors: Kang Hyo Jung , Yoon Sunghyun , Kim Koeun , Lee Young Ju
Published: June 2021

Enterococci, which are considered environmental mastitis-causing pathogens, have easily acquired aminoglycoside-resistant genes that encode various aminoglycoside-modifying enzymes (AME). Therefore, this study was conducted to compare the distribution of high-level aminoglycoside-resistant (HLAR) and multidrug-resistant (MDR) Enterococcus faecalis (E. faecalis) bacteria isolated from bulk tank milk in four dairy companies in Korea. Moreover, it analyzed the characteristics of their antimicrobial resistance genes and virulence factors. Among the 301 E. faecalis bacteria studied, 185 (61.5%) showed HLAR with no significant differences among the dairy companies. Furthermore, 129 (69.7%) of the 185 HLAR E. faecalis showed MDR without significant differences among companies. In contrast, HLAR E. faecalis from companies A, B, and C were significantly higher in resistance to the four classes than those in company D, which had the highest MDR ability against the three antimicrobial classes (p < 0.05). In addition, in the distribution of AME genes, 72 (38.9%) and 36 (19.5%) of the isolates carried both aac(6′)Ie-aph(2″)-la and ant(6)-Ia genes, and the ant (6)-Ia gene alone, respectively, with significant differences among the companies (p < 0.05). In the distribution of virulence genes, the ace (99.5%), efa A (98.9%), and cad 1 (98.4%) genes were significantly prevalent (p < 0.05). Thus, our results support that an advanced management program by companies is required to minimize the dissemination of antimicrobial resistance and virulence factors.
An Assessment of the Impact of Coronavirus Disease (COVID-19) Pandemic on National Antimicrobial Consumption in Jordan
Authors: Al-Azzam Sayer , Mhaidat Nizar Mahmoud , Banat Hayaa A. , Alfaour Mohammad , Ahmad Dana Samih , Muller Arno , Al-Nuseirat Adi , Lattyak Elizabeth A. , Conway Barbara R. , Aldeyab Mamoon A.
Published: June 2021

Coronavirus disease 2019 (COVID-19) has overlapping clinical characteristics with bacterial respiratory tract infection, leading to the prescription of potentially unnecessary antibiotics. This study aimed at measuring changes and patterns of national antimicrobial use for one year preceding and one year during the COVID-19 pandemic. Annual national antimicrobial consumption for 2019 and 2020 was obtained from the Jordan Food and Drug Administration (JFDA) following the WHO surveillance methods. The WHO Access, Watch, and Reserve (AWaRe) classification was used. Total antibiotic consumption in 2020 (26.8 DDD per 1000 inhabitants per day) decreased by 5.5% compared to 2019 (28.4 DDD per 1000 inhabitants per day). There was an increase in the use of several antibiotics during 2020 compared with 2019 (third generation cephalosporins (19%), carbapenems (52%), macrolides (57%), and lincosamides (106%)). In 2020, there was a marked reduction in amoxicillin use (−53%), while the use of azithromycin increased by 74%. National antimicrobial consumption of the Access group decreased by 18% from 2019 to 2020 (59.1% vs. 48.1% of total consumption). The use of the Watch group increased in 2020 by 26%. The study highlighted an increase in the use of certain antibiotics during the pandemic period that are known to be associated with increasing resistance. Efforts to enhance national antimicrobial stewardship are needed to ensure rational use of antimicrobials.
Effect of a Simvastatin-Impregnated Chitosan Scaffold on Cell Growth and Osteoblastic Differentiation
Authors: Alsawah Ghaliah M. , Al-Obaida Mohammad I. , Al-Madi Ebtissam M.
Published: June 2021

This study aims to evaluate the effect of chitosan (CS) scaffold, alone, and the potential synergistic effect when impregnated with simvastatin (SIM), on immortalized human bone-marrow mesenchymal stem cells (hbMMSCs) compared to CollaCote (CL). CS scaffolds were fabricated and seeded with immortalized hBMMSCs. Samples were divided into control groups (negative with no added material and positive with CL added) and four experimental groups: CS alone, CS/SIM 0.01, 0.03, and 0.05 mg, respectively. Cell viability, osteoblastic differentiation and calcium deposition were investigated via AlamarBlue, alkaline phosphate activity assays and alizarin red S staining at 1 and 14 days, respectively. At day one, no significant difference was noted between the groups regarding cell viability. However, all CS/SIM groups showed significant cutback at day 14 in cell proliferation compared to CS alone and CL groups (p < 0.001). All groups supported osteoblastic differentiation with no significant difference. Alkaline phosphate activity increased in both time periods in the CS/SIM 0.05 mg group compared to the other SIM groups, with no significant difference among the experimental groups. Chitosan scaffold is a bioactive compatible material capable of regenerative potential of hBMMSCs and a promising material to be used for perforation repair.
Adhesion Strength Change Analysis Based on the Application Surface Area Ratio of Spot-Bonded Tiles on Vertical Walls of High Humidity Facilities
Authors: Lee Jung-hun , Kim Bum-soo , Oh Kyu-hwan , Jiang Bo , He Xingyang , Kim Byoung-il , Oh Sang-keun
Published: June 2021

Facility walls with high relative humidity, such as bathrooms or kitchens installed with tiles by spot-bonding methods, become far more prone to defect or adhesion failure when using large or heavy tiles and insufficient application area of adhesive but is still continued to be practiced due to their low costs in the material. Most importantly, if this practice is to be continued, the changes in adhesion strength of the tiles based on different adhesive application areas of adhesives must be clarified such that the very least secure application can be achieved even by using spot-bonding methods. In this regard, an experiment was conducted in this study where tile-adhered specimens with different adhesive-applied area ratios (AR) of 60 ± 2%, 80 ± 2%, and 100% were prepared. Tile adhesion strength was subsequently measured, after sectioning the entire surface of the tile into 40 pieces. Experimental results showed that the adhesion strength above the standard criteria could be achieved for about 75% of the entire tile with AR 100% conditioning, followed by 30% of the entire tile with AR 80 ± 2% conditioning, and 20% of the entire tile for AR 60 ± 2% conditioning. Further analysis showed that with AR 80 ± 2% and AR 60 ± 2% conditions, the overall adhesion strength decreased by the range of about 59–67% compared to the AR 100% application conditions. The results of the study intended to provide an analytical basis of guidelines and risks with the potential usage of spot-bonding and should only be used if AR 100% application is planned.
Characteristics of Polycarbonate Soft Segment-Based Thermoplastic Polyurethane
Authors: Wu Chien-Hui , Chen Chin-Wen , Chen Po-Han , Chen Yi-Shin , Chuan Fu-Sheng , Rwei Syang-Peng
Published: June 2021

The pre-experiment of the present study revealed that polyurethane (PU) synthesized using poly (hexamethylene carbonate) glycol (PHC) has high melt viscosity and is difficult to process. Therefore, poly (trimethylene carbonate) glycol (PTC) was employed to synthesize a PU product with low melt viscosity. First, four types of thermoplastic polyurethane (TPU) were formed through one-step solvent-free synthesis. TPU is presented in the format “TPU-X-Y,” with X representing the polyol (PTC or PHC, around 1000 molecular weight) and Y the chain extender (1,3-propane diol [PDO] or 1,4-butane diol [BDO]) used. The TPU was synthesized using a fixed molar ratio of (isocyanate):(polyol):(chain extender) = 2:1:1 and compared. The results indicated that chain entanglement often occurred among the long carbon chains of PHC. The synthesized TPU employed a property of PTC, namely converting polarity into reverse polarity in high temperatures, to resolve the high melt viscosity of TPU of the PHC series, which causes processing difficulties. The synthesized TPU-PTC-PDO exhibited favorable molecular arrangements. Given its polarity, TPU-PTC-PDO has outstanding tensile properties (strength at break: 41.10 ± 10.78 MPa; 100% modulus = 6.73 ± 0.12 MPa), making processing at lower temperatures (180 or 190 °C) feasible. With the inclusion of PTC, the synthesized polycarbonate TPU exhibits the advantages of polycarbonate and is suitable for a wide range of applications.
Towards an Optical Gas Standard for Traceable Calibration-Free and Direct NO2 Concentration Measurements
Authors: Nwaboh Javis A. , Qu Zhechao , Werhahn Olav , Ebert Volker
Published: June 2021

We report a direct tunable diode laser absorption spectroscopy (dTDLAS) instrument developed for NO2 concentration measurements without chemical pre-conversion, operated as an Optical Gas Standard (OGS). An OGS is a dTDLAS instrument that can deliver gas species amount fractions (concentrations), without any previous or routine calibration, which are directly traceable to the international system of units (SI). Here, we report NO2 amount fraction quantification in the range of 100–1000 µmol/mol to demonstrate the current capability of the instrument as an OGS for car exhaust gas application. Nitrogen dioxide amount fraction results delivered by the instrument are in good agreement with certified values of reference gas mixtures, validating the capability of the dTDLAS-OGS for calibration-free NO2 measurements. As opposed to the standard reference method (SRM) based on chemiluminescence detection (CLD) where NO2 is indirectly measured after conversion to NO, titration with O3 and the detection of the resulting fluorescence, a dTDLAS-OGS instrument has the benefit of directly measuring NO2 without distorting or delaying conversion processes. Therefore, it complements the SRM and can perform fast and traceable measurements, and side-by-side calibrations of other NO2 gas analyzers operating in the field. The relative standard uncertainty of the NO2 results reported in this paper is 5.1% (k = 1, which is dominated (98%) by the NO2 line strength), the repeatability of the results at 982.6 µmol/mol is 0.1%, the response time of the instrument is 0.5 s, and the detection limit is 825 nmol/mol at a time resolution of 86 s.
Perspectives on Cathodes for Protonic Ceramic Fuel Cells
Authors: Mather Glenn C. , Muñoz-Gil Daniel , Zamudio-García Javier , Porras-Vázquez José M. , Marrero-López David , Pérez-Coll Domingo
Published: June 2021

Protonic ceramic fuel cells (PCFCs) are promising electrochemical devices for the efficient and clean conversion of hydrogen and low hydrocarbons into electrical energy. Their intermediate operation temperature (500–800 °C) proffers advantages in terms of greater component compatibility, unnecessity of expensive noble metals for the electrocatalyst, and no dilution of the fuel electrode due to water formation. Nevertheless, the lower operating temperature, in comparison to classic solid oxide fuel cells, places significant demands on the cathode as the reaction kinetics are slower than those related to fuel oxidation in the anode or ion migration in the electrolyte. Cathode design and composition are therefore of crucial importance for the cell performance at low temperature. The different approaches that have been adopted for cathode materials research can be broadly classified into the categories of protonic–electronic conductors, oxide-ionic–electronic conductors, triple-conducting oxides, and composite electrodes composed of oxides from two of the other categories. Here, we review the relatively short history of PCFC cathode research, discussing trends, highlights, and recent progress. Current understanding of reaction mechanisms is also discussed.
Automatic Microscopy Analysis with Transfer Learning for Classification of Human Sperm
Authors: Liu Rui , Wang Mingmei , Wang Min , Yin Jianqin , Yuan Yixuan , Liu Jun
Published: June 2021

Infertility is a global problem that affects many couples. Sperm analysis plays an essential role in the clinical diagnosis of human fertility. The examination of sperm morphology is an essential technique because sperm morphology is a proven indicator of biological functions. At present, the morphological classification of human sperm is conducted manually by medical experts. However, manual classification is laborious and highly dependent on the experience and capability of clinicians. To address these limitations, we propose a transfer learning method based on AlexNet to automatically classify the sperms into four different categories in terms of the World Health Organization (WHO) standards by analyzing their morphology. We adopt the feature extraction architecture of AlexNet as well as its pre-training parameters. Besides, we redesign the classification network by adding the Batch Normalization layers to improve the performance. The proposed method achieves an average accuracy of 96.0% and an average precision of 96.4% in the freely-available HuSHeM dataset, which exceeds the performance of previous algorithms. Our method shows that automatic sperm classification has great potential to replace manual sperm classification in the future.
Seasonal to Interannual Variability of Vertical Wind Shear and Its Relationship with Tropical Cyclogenesis in the Mozambique Channel
Authors: Manhique Atanásio João , Guirrugo Isac Arnaldo , Nhantumbo Bernardino João , Mavume Alberto Francisco
Published: June 2021

This article explores the relationship between vertical wind shear (VWS) and tropical cyclone (TC) genesis in the Mozambique Channel (MC) for the period 1979–2019. Additionally, SST, low-level relative vorticity, 700 hPa relative humidity and upper-level divergence were also analyzed for the peak cyclogenesis months to explore their relative contributions. The analyses were done using NCEP/NCAR Reanalysis-1 for the atmospheric fields, monthly Optimum Interpolation SST V2, and for the cyclogenesis the TC best track data from the La Reunion–Regional Specialized Meteorological and Joint Typhoon Warning Centre. A total of 43 TCs generated in the MC were observed for the analysed period. The maximum frequency of cyclogenesis in the MC was observed during January and February and the spatial location of maximum TC genesis was coincident with the minimum values of the VWS. The VWS showed significant correlations with TC intensity, particularly when considering the upper atmosphere (200–500 hPa) or the bulk (200–850 hPa) VWS. The mean composites of the cyclogenesis months over the MC of SST, relative humidity at 700 hPa, divergence at upper atmosphere, showed significant values. However, linear correlations between these factors vs. TC genesis frequency and intensity were not significant. Analyses of interannual correlations between VWS and Niño-3.4 (subtropical southwest Indian Ocean dipole-SIOD) showed statistically significant positive (negative) correlations at different lags, suggesting that La Niña and the positive phase of SIOD conditions are favorable to weaker VWS and thus to intensification of TCs in the Mozambique Channel. Thirteen landfall cases were observed with seven over the Madagascar west coast and six over the Mozambique coast. The landfall over the Madagascar (Mozambique) coast was associated with strengthened (weakened) VWS.
Source Apportionment and Toxicity of PM in Urban, Sub-Urban, and Rural Air Quality Network Stations in Catalonia
Authors: Jaén Clara , Villasclaras Paula , Fernández Pilar , Grimalt Joan O. , Udina Mireia , Bedia Carmen , van Drooge Barend L.
Published: June 2021

Air quality indicators, i.e., PM10, NO2, O3, benzo[a]pyrene, and several organic tracer compounds were evaluated in an urban traffic station, a sub-urban background station, and a rural background station of the air quality network in Catalonia (Spain) from summer to winter 2019. The main sources that contribute to the organic aerosol and PM toxicity were determined. Traffic-related air pollution dominated the air quality in the urban traffic station, while biomass burning in winter and secondary organic aerosol (SOA) in summer impact the air quality in the sub-urban and rural background stations. Health risk assessment for chronic exposure over the past decade, using WHO air quality standards, showed that NO2, PM10 and benzo[a]pyrene from traffic emissions pose an unacceptable risk to the human population in the urban traffic station. PM10 and benzo[a]pyrene from biomass burning were unacceptably high in the sub-urban and rural background stations. Toxicity tests of the PM extracts with epithelial lung cells showed higher toxicity in wintertime samples in the sub-urban and rural stations, compared to the urban traffic station. These results require different mitigation strategies for urban and rural sites in order to improve the air quality. In urban areas, traffic emissions are still dominating the air quality, despite improvements in the last years, and may directly be responsible for part of the SOA and O3 levels in sub-urban and rural areas. In these later areas, air pollution from local biomass burning emissions are dominating the air quality, essentially in the colder period of the year.
The Phenomenon of Diurnal Variations for Summer Deep Convective Precipitation over the Qinghai-Tibet Plateau and Its Southern Regions as Viewed by TRMM PR
Authors: Luo Jing , Zheng Jianqiu , Zhong Lei , Zhao Chun , Fu Yunfei
Published: June 2021

This study analyzed the diurnal variations of summer deep convective precipitation (DCP) over the Qinghai-Tibet Plateau (QTP) and its southern region. The results show that DCP is the main type of precipitation over the QTP. The precipitation intensity of DCP is less than 3 mm/h over the QTP, which is much lower than the precipitation intensity in non-plateau regions. DCP over non-plateau regions is related to the convergence of surface wind, but that over the QTP are not. The mean maximum echo of DCP is less than 26 dBZ over the QTP, less than in non-plateau regions. The mean altitude of maximum echo decreases from about 7.5 km in the western plateau to 6 km in the eastern plateau, while it reaches only 4.5–5 km in the non-plateau region. The DCP frequency peak occurs in the afternoon in the major area of the QTP including valley region. The peak time of DCP frequency is different from its intensity, and the former is 1 to 2 h earlier. Study also indicates strong diurnal variations in frequency, intensity, and the maximum echo over the QTP, which is consistent with diurnal changes of geopotential height fields of 500 hPa and 200 hPa.
Few Body Effects in the Electron and Positron Impact Ionization of Atoms
Authors: Campeanu R.I. , Whelan Colm T.
Published: June 2021

Triple differential cross sections (TDCS) are presented for the electron and positron impact ionization of inert gas atoms in a range of energy sharing geometries where a number of significant few body effects compete to define the shape of the TDCS. Using both positrons and electrons as projectiles has opened up the possibility of performing complementary studies which could effectively isolate competing interactions that cannot be separately detected in an experiment with a single projectile. Results will be presented in kinematics where the electron impact ionization appears to be well understood and using the same kinematics positron cross sections will be presented. The kinematics are then varied in order to focus on the role of distortion, post collision interaction (pci), and interference effects.
Imaging of Substantia Nigra in Parkinson’s Disease: A Narrative Review
Authors: Feraco Paola , Gagliardo Cesare , La Tona Giuseppe , Bruno Eleonora , D’angelo Costanza , Marrale Maurizio , Del Poggio Anna , Malaguti Maria Chiara , Geraci Laura , Baschi Roberta , Petralia Benedetto , Midiri Massimo , Monastero Roberto
Published: June 2021

Parkinson’s disease (PD) is a progressive neurodegenerative disorder, characterized by motor and non-motor symptoms due to the degeneration of the pars compacta of the substantia nigra (SNc) with dopaminergic denervation of the striatum. Although the diagnosis of PD is principally based on a clinical assessment, great efforts have been expended over the past two decades to evaluate reliable biomarkers for PD. Among these biomarkers, magnetic resonance imaging (MRI)-based biomarkers may play a key role. Conventional MRI sequences are considered by many in the field to have low sensitivity, while advanced pulse sequences and ultra-high-field MRI techniques have brought many advantages, particularly regarding the study of brainstem and subcortical structures. Nowadays, nigrosome imaging, neuromelanine-sensitive sequences, iron-sensitive sequences, and advanced diffusion weighted imaging techniques afford new insights to the non-invasive study of the SNc. The use of these imaging methods, alone or in combination, may also help to discriminate PD patients from control patients, in addition to discriminating atypical parkinsonian syndromes (PS). A total of 92 articles were identified from an extensive review of the literature on PubMed in order to ascertain the-state-of-the-art of MRI techniques, as applied to the study of SNc in PD patients, as well as their potential future applications as imaging biomarkers of disease. Whilst none of these MRI-imaging biomarkers could be successfully validated for routine clinical practice, in achieving high levels of accuracy and reproducibility in the diagnosis of PD, a multimodal MRI-PD protocol may assist neuroradiologists and clinicians in the early and differential diagnosis of a wide spectrum of neurodegenerative disorders.
Impact of Neoantigen Expression and T-Cell Activation on Breast Cancer Survival
Authors: Li Wenjing , Amei Amei , Bui Francis , Norouzifar Saba , Lu Lingeng , Wang Zuoheng
Published: June 2021

Neoantigens are derived from tumor-specific somatic mutations. Neoantigen-based synthesized peptides have been under clinical investigation to boost cancer immunotherapy efficacy. The promising results prompt us to further elucidate the effect of neoantigen expression on patient survival in breast cancer. We applied Kaplan–Meier survival and multivariable Cox regression models to evaluate the effect of neoantigen expression and its interaction with T-cell activation on overall survival in a cohort of 729 breast cancer patients. Pearson’s chi-squared tests were used to assess the relationships between neoantigen expression and clinical pathological variables. Spearman correlation analysis was conducted to identify correlations between neoantigen expression, mutation load, and DNA repair gene expression. ERCC1, XPA, and XPC were negatively associated with neoantigen expression, while BLM, BRCA2, MSH2, XRCC2, RAD51, CHEK1, and CHEK2 were positively associated with neoantigen expression. Based on the multivariable Cox proportional hazard model, patients with a high level of neoantigen expression and activated T-cell status showed improved overall survival. Similarly, in the T-cell exhaustion and progesterone receptor (PR) positive subgroups, patients with a high level of neoantigen expression showed prolonged survival. In contrast, there was no significant difference in the T-cell activation and PR negative subgroups. In conclusion, neoantigens may serve as immunogenic agents for immunotherapy in breast cancer.